bluuebunny/crossref_metadata_2025 huggingface dataset:
Active Prevalence of Fusarium falciforme and F. acutatum Causing Basal Rot of Onion in Maharashtra, India
Authors: Dutta Ram , Jayalakshmi Krishnappa , Radhakrishna Auji , Kumar Satish , Mahajan Vijay
Published: June 2024

Over the past decade, there have been accumulating reports from researchers, farmers, and field extension personnel on the increasing incidence and spread of onion basal rot in India. Onion basal rot disease is mainly caused by Fusarium spp. This study aimed to validate the information on the active prevalence of F. falciforme and F. acutatum causing Fusarium basal rot (FBR) in Maharashtra. A survey was conducted, and the infected plants/bulbs were collected from fields of 38 locations comprising five districts of Maharashtra, namely, Nashik, Aurangabad, Solapur, Ahmednagar, and Pune, in 2023. This disease was prevalent in high-moisture and high-oil-temperature conditions and the symptoms were observed in most of the fields, with the FBR incidence ranging from 17 to 41%. The available data of basal rot incidence from 1998 to 2022 were analyzed, based on which the prevalence of FBR was 11–50%. Tissue from the infected samples of onion bulbs was used for the isolation. The identification was performed based on colony morphology and microscopic features and confirmed through molecular markers using ITS and Tef-1α gene primers. Of the ten Fusarium isolates collected from selected locations, six species were confirmed as F. acutatum and four as F. falciforme. The pathogenicity tests performed with onion seedlings and bulbs under moist conditions proved that both F. acutatum and F. falciforme independently could cause basal rot disease symptoms but with different degrees of virulence. Koch’s postulates were confirmed by reisolating the same pathogens from the infected plants. Thus, the active prevalence of FBR was confirmed in Maharashtra and also, to the best of our knowledge, this is the first report of F. falciforme and F. acutatum causing basal rot of onion independently in Maharashtra, India.
Association between Height and Functional Outcomes of Patients 6 Months after a Stroke: A Multicenter Prospective Observational Cohort Study
Authors: Kang Nae Yoon , Ko Sung-Hwa , Shin Yong-Il , Min Ji Hong , Yun Mi Sook , Sohn Min Kyun , Lee Jongmin , Kim Deog Young , Oh Gyung-Jae , Lee Yang Soo , Joo Min Cheol , Lee So Young , Song Min-Keun , Han Junhee , Ahn Jeonghoon , Kim Yun-Hee , Chang Won Hyuk
Published: June 2024

Many physical factors influence post-stroke functional outcomes. However, few studies have examined the influence of height on these outcomes. Here, data from the Korean Stroke Cohort for Functioning and Rehabilitation were used and patients’ height was categorized into three groups: short (lower 25%), middle (middle 50%), and tall (upper 25%). Differences in the modified Rankin scale (mRS), functional ambulatory category (FAC), and Korean-translated version of the Modified Barthel Index (K-MBI) scores were analyzed for each group at 6 months post-stroke. A subgroup analysis was conducted based on the initial Fugl-Meyer Assessment (FMA) score. We analyzed functional outcomes in 5296 patients at 6 months post-stroke, adjusting for age and body mass index. The short-height group exhibited higher mRS scores (1.88 ± 0.043), lower FAC scores (3.74 ± 0.045), and lower K-MBI scores (82.83 ± 0.748) than the other height groups (p < 0.05). In the subgroup analysis, except for the very severe FMA group, the short-height group also exhibited worse outcomes in terms of mRS, FAC, and K-MBI scores (p < 0.05). Taken together, the short-height group exhibited worse outcomes related to disability, gait function, and ADLs at 6 months post-stroke.
The Two Levels of Podocyte Dysfunctions Induced by Apolipoprotein L1 Risk Variants
Authors: Pays Etienne
Published: June 2024

Apolipoprotein L1 (APOL1) nephropathy results from several podocyte dysfunctions involving morphological and motility changes, mitochondrial perturbations, inflammatory stress, and alterations in cation channel activity. I propose that this phenotype results from increased hydrophobicity of the APOL1 risk variants, which induces two distinct types of podocyte dysfunctions. On one hand, increased hydrophobic interactions with APOL3 cause intracellular variant isoforms to impair both APOL3 control of Golgi PI(4)P kinase-B (PI4KB) activity and APOL3 control of mitochondrial membrane fusion, triggering actomyosin reorganisation together with mitophagy and apoptosis inhibition (hit 1). On the other hand, increased hydrophobic interactions with the podocyte plasma membrane may cause the extracellular variant isoforms to activate toxic Ca2+ influx and K+ efflux by the TRPC6 and BK channels, respectively (hit 2), presumably due to APOL1-mediated cholesterol clustering in microdomains. I propose that hit 2 depends on low HDL-C/high extracellular APOL1 ratio, such as occurs in cell culture in vitro, or during type I-interferon (IFN-I)-mediated inflammation.
Editorial of the Special Issue Digital Soil Mapping, Decision Support Tools and Soil Monitoring Systems in the Mediterranean
Authors: Lo Papa Giuseppe , Schillaci Calogero , Fantappiè Maria , Langella Giuliano
Published: June 2024

In the digital era, the role of soil surveyors has evolved significantly [...]
The Intersection of the Pathogenic Processes Underlying Psoriasis and the Comorbid Condition of Obesity
Authors: Scala Emanuele , Mercurio Laura , Albanesi Cristina , Madonna Stefania
Published: June 2024

In the past decade, our understanding of psoriasis pathogenesis has made significant steps forward, leading to the development of multiple game-changing therapies. While psoriasis primarily affects the skin, it is increasingly recognized as a systemic disease that can have effects beyond the skin. Obesity is associated with more severe forms of psoriasis and can potentially worsen the systemic inflammation and metabolic dysfunction seen in psoriatic patients. The exact mechanisms underlying the link between these two conditions are not fully understood, but it is believed that chronic inflammation and immune dysregulation play a role. In this review, we examine the existing body of knowledge regarding the intersection of pathogenic processes responsible for psoriasis and obesity. The ability of biological therapies to reduce systemic and obesity-related inflammation in patients with psoriasis will be also discussed.
A Decrease in the Hardness of Feces with Added Glucosylceramide Extracted from Koji In Vitro—A Working Hypothesis of Health Benefits of Dietary Glucosylceramide
Authors: Dai Huanghuang , Hariwitonang Johan , Fujiyama Nao , Moriguchi Chihiro , Hirano Yuto , Ebara Fumio , Inaba Shigeki , Kondo Fumiyoshi , Kitagaki Hiroshi
Published: June 2024

Skin barrier function, prevent colon cancer, head and neck cancer, and decrease liver cholesterol. However, the mechanism of action has not yet been elucidated. In this study, we propose a new working hypothesis regarding the health benefits and functions of glucosylceramide: decreased fecal hardness. This hypothesis was verified using an in vitro hardness test. The hardness of feces supplemented with glucosylceramide was significantly lower than that of the control. Based on these results, a new working hypothesis of dietary glucosylceramide was conceived: glucosylceramide passes through the small intestine, interacts with intestinal bacteria, increases the tolerance of these bacteria toward secondary bile acids, and decreases the hardness of feces, and these factors synergistically result in in vivo effects. This hypothesis forms the basis for further studies on the health benefits and functions of dietary glucosylceramides.
[(N-benzamidomethyl)(N-benzoyl)amino]methyltriphenylphosphonium Tetrafluoroborate
Authors: Adamek Jakub , Kaczmarczyk Wiktoria , Sapia Dawid
Published: June 2024

In this study, [(N-benzamidomethyl)(N-benzoyl)amino]methyltriphenylphosphonium tetrafluoroborate was synthesized at 80 °C, starting from N-benzoylaminomethyltriphenylphosphonium tetrafluoroborate, by a specific α-amidoalkylation reaction using Hünig’s base as a catalyst. N-benzoylaminomethyltriphenylphosphonium tetrafluoroborate acts as both an amidoalkylating agent and a nucleophile precursor. The structure of the compound obtained was confirmed by spectroscopic methods (1H-, 13C-, 31P-NMR, IR) and HR-MS analysis.
Prediction of Residual Life of In-Service P91 Steel Joints Based on Fracture Behavior
Authors: Yan Kai , Cai Yongjiang , Wang Denghui , Zhang Shichao , Yi Shuang , Yang Fulai , Zhang Zheng
Published: June 2024

P91 steel and P91 steel joints experience performance degradation after serving for 30,000 h in working conditions. To clarify the damage and failure behavior and remaining life of the joints during subsequent service, further creep testing was conducted on the welded joints of P91 steel that had been in service for 30,000 h at three temperatures: 550 °C, 575 °C, and 600 °C. The fracture surface and the cross-section damage behavior were characterized by SEM and EBSD methods. The results show that there are two types of fracture modes in the joints at different temperatures: ductile cracking occurring at the BM, and type IV cracking occurring in the FGHAZ. The threshold stress for fracture mode transition decreases with an increase in working temperature. Type IV cracking near the HAZ is the main reason for the premature failure of joints during service. And based on the fracture mode, the dual-constant L-M method was proposed to predict the strength of in-service joint materials. The testing data are discussed and classified based on the fracture mode in this method, which has high accuracy and can prevent the premature failure of joints.
Effects of Micro- and Nanosilica on the Mechanical and Microstructural Characteristics of Some Special Mortars Made with Recycled Concrete Aggregates
Authors: Mazilu Claudiu , Deju Radu , Georgescu Dan Paul , Apostu Adelina , Barbu Alin
Published: June 2024

In this paper, we study the influence of densified microsilica and colloidal nanosilica admixtures on the mechanical strength and the microstructural characteristics of special mortars used for immobilizing radioactive concrete waste. The experimental program focused on the replacement of cement with micro- and/or nanosilica, in different proportions, in the basic composition of a mortar made with recycled aggregates. The technical criteria imposed for such cementitious systems, used for the encapsulation of low-level radioactive waste, imply high fluidity, increased mechanical strength and lack of segregation and of bleeding. We aimed to increase the structural compactness of the mortars by adding micro- and nanosilica, all the while maintaining the technical criteria imposed, to obtain a cement matrix with high durability and increased capacity for immobilizing radionuclides. The samples from all the compositions obtained were analyzed from the point of view of mechanical strength. Also, micro- and nanosilica as well as samples of the optimal mortar compositions were analyzed physically and microstructurally. Experimental data showed that the mortar samples present maximum compressive strength for a content between 6 and 7.5% wt. of microsilica, respectively, for a content of 2.25% wt. nanosilica. The obtained results suggest a synergistic effect of micro- and nanosilica when they are used simultaneously in cementitious compositions. Thus, among the analyzed compositional variants, the mortar composition with 3% wt. microsilica and 2.25% wt. nanosilica showed the best performance, with an increase in compressive strength of 23.5% compared to the control sample (without micro- and nanosilica). Brunauer–Emmett–Teller (BET) analysis and scanning electron microscopy (SEM) images highlighted the decrease in pore diameter and the increase in structural compactness, especially for mortar samples with nanosilica content or a mixture of micro- and nanosilica. This study is useful in the field of recycling radioactive concrete resulting from the decommissioning of nuclear research or nuclear power reactors.
Thermoplastic Polyurethane-poly(N-isopropylacrylamide) Copolymer for Selective Uptake of Alcohol from Aqueous Solution
Authors: Wang Fei , Cheng Tiexin , Zhou Guangdong
Published: June 2024

Ethanol possesses high economic value, but as an industrial waste, it poses harm to human health and the environment. The paper describes the preparation of a thermoplastic polyurethane-poly(n-isopropylacrylamide) (TPU-PNIPAM) copolymer designed to selectively uptake alcohol in aqueous solution. The material was created by bonding TPU and PNIPAM together through intermolecular hydrogen bonds, enhancing its hydrophobic properties and making it easier to interact with alcohol molecules. As the amount of PNIPAM in TPU increases, the number of hydrophobic isopropyl groups in TPU-PNIPAM also increases, leading to an enhanced selective uptake ability of TPU-PNIPAM for alcohols in aqueous solution. When the temperature reaches 55 °C, the hydrophobic groups in TPU-PNIPAM are more exposed, further enhancing the selective uptake ability of TPU-PNIPAM for alcohols in aqueous solution. TPU-PNIPAM demonstrates selective preferential uptake for various concentrations and types of alcohol in aqueous solutions. The material’s selective uptake performance for alcohols increases with their hydrophobicity, so TPU-PNIPAM exhibited the best adsorption performance for a 10 wt% n-propanol solution under the combined effect of steric hindrance. In addition, TPU-PNIPAM exhibited selective adsorption for other organic solvents, which demonstrated the universality of TPU-PNIPAM in removing contaminants from aqueous solutions.
Oxidation Performance of Nano-Layered (AlTiZrHfTa)Nx/SiNx Coatings Deposited by Reactive Magnetron Sputtering
Authors: Touaibia Djallel Eddine , Achache Sofiane , Bouissil Abdelhakim , Parent Fabrice , Ghanbaja Jaafar , Gorbunova Alina , Postnikov Pavel S. , Chehimi Mohamed Mehdi , Schuster Frederic , Sanchette Frederic , El Garah Mohamed
Published: June 2024

This work uses the direct current magnetron sputtering (DCMS) of equi-atomic (AlTiZrHfTa) and Si targets in dynamic sweep mode to deposit nano-layered (AlTiZrHfTa)Nx/SiNx refractory high-entropy coatings (RHECs). Transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), thermogravimetric analysis (TGA), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) are used to investigate the effect of Si addition on the oxidation behavior of the nano-layered coatings. The Si-free nitride coating exhibits FCC structure and columnar morphology, while the Si-doped nitride coatings present a FCC (AlTiZrHfTa)N/amorphous-SiNx nano-layered architecture. The hardness decreases from 24.3 ± 1.0 GPa to 17.5 ± 1.0 GPa because of the nano-layered architecture, whilst Young’s modulus reduces from 188.0 ± 1.0 GPa to roughly 162.4 ± 1.0 GPa. By increasing the thickness of the SiNx nano-layer, kp values decrease significantly from 3.36 × 10−8 g2 cm−4 h−1 to 6.06 × 10−9 g2 cm−4 h−1. The activation energy increases from 90.8 kJ·mol−1 for (AlTiZrHfTa)Nx nitride coating to 126.52 kJ·mol−1 for the (AlTiZrHfTa)Nx/SiNx nano-layered coating. The formation of a FCC (AlTiZrHfTa)-Nx/a-SiNx nano-layered architecture results in the improvement of the resistance to oxidation at high temperature.
17-4 PH Steel Parts Obtained through MEX and PBF-LB/M Technologies: Comparison of the Structural Properties
Authors: Jasik Katarzyna , Śnieżek Lucjan , Kluczyński Janusz , Łuszczek Jakub , Grzelak Krzysztof , Sarzyński Bartłomiej , Szachogłuchowicz Ireneusz
Published: June 2024

The material extrusion (MEX) method utilizing highly filled metal filament presents an alternative to advanced additive metal manufacturing technologies. This process enables the production of metal objects through deposition and sintering, which is particularly attractive compared to powder bed fusion (PBF) technologies employing lasers or high-power electron beams. PBF requires costly maintenance, skilled operators, and controlled process conditions, whereas MEX does not impose such requirements. This study compares research on 17-4 PH steel manufactured using two different commercially available techniques: MEX and powder bed fusion with laser beam melting (PBF-LB/M). This research included assessing the density of printed samples, analyzing surface roughness in two printing planes, examining microstructure including porosity and density determination, and measuring hardness. The conducted research aimed to determine the durability and quality of the obtained samples and to evaluate their strength. The research results indicated that samples produced using the PBF-LB/M technology exhibited better density and a more homogeneous structure. However, MEX samples exhibited better strength properties (hardness).
Research and Implementation of Pneumatic Amphibious Soft Bionic Robot
Authors: Zhao Wenchuan , Zhang Yu , Yang Lijian , Wang Ning , Peng Linghui
Published: June 2024

To meet the requirements of amphibious exploration, ocean exploration, and military reconnaissance tasks, a pneumatic amphibious soft bionic robot was developed by taking advantage of the structural characteristics, motion forms, and propulsion mechanisms of the sea lion fore-flippers, inchworms, Carangidae tails, and dolphin tails. Using silicone rubber as the main material of the robot, combined with the driving mechanism of the pneumatic soft bionic actuator, and based on the theory of mechanism design, a systematic structural design of the pneumatic amphibious soft bionic robot was carried out from the aspects of flippers, tail, head–neck, and trunk. Then, a numerical simulation algorithm was used to analyze the main executing mechanisms and their coordinated motion performance of the soft bionic robot and to verify the rationality and feasibility of the robot structure design and motion forms. With the use of rapid prototyping technology to complete the construction of the robot prototype body, based on the motion amplitude, frequency, and phase of the bionic prototype, the main execution mechanisms of the robot were controlled through a pneumatic system to carry out experimental testing. The results show that the performance of the robot is consistent with the original design and numerical simulation predictions, and it can achieve certain maneuverability, flexibility, and environmental adaptability. The significance of this work is the development of a pneumatic soft bionic robot suitable for amphibious environments, which provides a new idea for the bionic design and application of pneumatic soft robots.
Recyclability Perspectives of the Most Diffused Biobased and Biodegradable Plastic Materials
Authors: Coltelli Maria-Beatrice , Gigante Vito , Aliotta Laura , Lazzeri Andrea
Published: June 2024

The present chapter focuses on the recyclability of both renewable and biodegradable plastics, considering the recovery of matter (mechanical or chemical recycling) from the polymeric materials currently most diffused on the market. Biobased and compostable plastics are carbon neutral; thus, they do not contribute significantly to greenhouse gas (GHG) emissions. Nevertheless, recycling can be beneficial because it allows a prolongation of the material life cycle so that carbon is stored for a longer time up to the final composting. The chemical or mechanical recycling option is linked both to the possibility of reprocessing bioplastics without detrimental loss of properties as well as to the capability of selecting homogenous fractions of bioplastics after waste collection. Moreover, the different structural features of biodegradable bioplastics have resulted in different chemical recycling opportunities and also in different behaviors during the reprocessing operations necessary for recycling. All these aspects are discussed systematically in this review, considering biodegradable bioplastics, their blends and composites with natural fibers.
Developing and Evaluating the Operating Region of a Grid-Connected Current Source Inverter from Its Mathematical Model
Authors: Baier Carlos R. , Melin Pedro E. , Torres Miguel A. , Ramirez Roberto O. , Muñoz Carlos , Quinteros Agustin
Published: June 2024

Grid-connected power inverters are indispensable in modern electrical systems, playing a pivotal role in enhancing the integration of renewable energies into power grids. Their significance, primarily when functioning as grid-forming inverters, extends to maintaining the grid’s inertia and strength—a distinct advancement over traditional grid-following operations. As grid-forming inverters, these devices emulate the characteristics of synchronous generators and can act as robust voltage sources, providing essential ancillary services. This behavior is particularly relevant when integrating energy storage systems on the converters’ direct current side. Among the various inverter topologies, the current source inverter (CSI) has emerged as a promising yet underexplored alternative for grid-forming applications. CSIs, when paired with their AC output filters, can effectively operate as voltage sources, utilizing control strategies that facilitate the integration of renewable energies into the electrical system. Their design inherently manages output current fluctuations, reducing the need for restrictive current limitations or additional protective measures. This paper examines the operational region of CSIs, obtained through detailed modeling, to explore their advantages, challenges, and potential for enhancing grid-connected systems. Analyzing the operating region from the converter model verifies the limits of where the converter can operate in a plane of active and reactive powers. For a small prototype model operating with 7 amperes in DC and 120 V in AC, it is possible to supply or absorb active power exceeding 1000 W and manage maximum reactive power values around 500 VAr, as determined by its operating region. Simulations also confirm that small changes in the control reference, as little as 5%, towards the region’s right limits cause significant oscillations in the dynamic control responses. This research aims to deepen our understanding of CSIs’ operational capabilities and highlight their unique benefits in advancing grid-connected systems and promoting the integration of renewable energy using this technology.
Semantic and Morphosyntactic Differences among Nouns: A Template-Based and Modular Cognitive Model
Authors: El Idrissi Mohamed
Published: June 2024

The noun category exhibits diverse dissimilarities, characterised at the semantic (e.g., countable/uncountable) or/and morphosyntactic (e.g., determined/determinerless) level, which may be more or less important depending on languages. In this paper, we would like to discuss those differences, which we named inter-word and inter-process morphosyntactic variations. The Riffian language served us as a reference in our enquiries, before referring to other languages to show how our discoveries could be applied to them. By putting in perspective those aspects, this led us to propose a formal mathematical model denoted as a Template-Based and Modular Cognitive model. The latter is able to predict the nonlinear dynamic mapping of lexical items onto morphological templates. The aims of this article are thus manifold and cover theoretical issues. We demonstrate that nouns are organised and distributed in modular cognitive sets, having their own morphological template and unmarked forms. The extent of these sets and their number as well as the template, are specific to each language. All sorts of markers can compose with the template, but some, namely countability markers, are prevalent among several languages with no relationship. This approach allows us to explain the marking discrepancies existing between different kinds of nouns (borrowed, proper, countable and uncountable nouns) for a given linguistic variety or between languages. The main assumption of this model is that these irregular markings are caused by a template shift, occurring when items undergo a process of word and meaning formation. Our contribution represents an initial stride toward understanding the fundamental patterns of morphosyntax and opens venues for applying this mathematical model with other behavioural and natural phenomena.
Monotonicity Results of Ratios between Normalized Tails of Maclaurin Power Series Expansions of Sine and Cosine
Authors: Niu Da-Wei , Qi Feng
Published: June 2024

In the paper, the authors establish the monotonicity results of the ratios between normalized tails of the Maclaurin power series expansions of the sine and cosine functions and restate them in terms of the generalized hypergeometric functions.
Influence of Incident Orientation on the Dynamic Response of Deep U-Shaped Cavern Subjected to Transient Loading
Authors: Liang Lisha , Li Xibing , Liu Zhixiang , Peng Siyu
Published: June 2024

In deep rock engineering, caverns are often disturbed by engineering loads from different directions. To investigate the dynamic response of deep U-shaped caverns under different incident orientations, a theoretical solution of the dynamic stress concentration factor along the cavern boundary was derived based on the wave function expansion and conformal mapping method, and the failure characteristics around the cavern were further investigated by PFC2D (Particle Flow Code in two dimensions). As the incident orientation increases from 0° to 90°, the dynamic compressive stress concentration area transforms from both the roof and the floor to the sidewalls, and the peak dynamic stress concentration factor of the roof decreases from 2.98 to −0.20. The failure of the floor converts from dynamic compression shear failure to dynamic tensile failure. Compared to a stress wave incident from the curved boundary, a stress wave incident from the flat boundary causes severer damage. When the stress wave is incident from the sidewall, the cavern with a larger height-to-width (h/w) ratio exhibits severer damage. Conversely, the cavern with a smaller h/w ratio tends to fail as the stress wave is incident from the floor. This paper provides a basic understanding of dynamic responses of the deep U-shaped cavern.
Supply Chain Elastic Strain
Authors: Yang Zihui , Meng Qingchun , Fang Zheng , Zhang Xiaona
Published: June 2024

The introduction of the concepts of shear elastic strain (ε−) and tensile elastic strain (ε+) is a catalyst for new horizons of research into supply chain elasticity. Functional formulas encompassing the metrics of ε− and ε+, their critical point, maximum strain value, and similar parameters are established through rigorous mathematical derivations. The supply chain elasticity of agricultural commodities, including grains, apples, and wheat, are assessed by utilizing the derived formulas. The results show that the metrics of supply chain elastic strain serve as direct metrics of measuring the supply chain’s anti-interference capability, and they also facilitate an objective assessment of the supply chain’s safety and stability. The formula is succinctly derived, and it yields objective outcomes with general applicability, particularly suited for research and application for supply chain elasticity.
Analysis of the Effect of Exams on Perceived Stress and Temporomandibular Joint Status in Physiotherapy Students: A Pilot Study
Authors: Paz-Cortés Marta Macarena , Martín-Vacas Andrea , Mendoza Pedro Antonio , Rozalén Manuel , Alvaredo Miguel Ángel
Published: June 2024

Background and Objectives: Temporomandibular disorders or dysfunction (TMDs) encompass a range of complex conditions that impact the temporomandibular joint (TMJ), chewing muscles, teeth, and/or their supporting tissues. Stress is one of the most associated factors studied as a TMD predictor. The aim is to figure out the influence of stress on TMJ status and salivary cortisol in university students during and before exams. Materials and Methods: The study was non-experimental, employing a longitudinal, analytical, observational cohort design. The study population consisted of students enrolled in the physiotherapy degree program at the Alfonso X El Sabio University (Madrid, Spain). Data were collected during two distinct academic periods: the first period was characterized by low academic stress and no exams, and the second period coincided with the high academic stress of final course exams. The collected results included sociodemographic data, assessment of TMJ status (Fonseca Index), evaluation of muscle evaluation (masseter, upper trapezius, and sternocleidomastoid) using a MOXY Monitor (muscle oxygenation) and Neurotrac® (surface EMG, sEMG), assessment of perceived stress (PSS-14), and measurement of salivary cortisol (enzyme immunoassay with Elisa). The statistical analysis was conducted with a confidence level of 95% (p ≤ 0.05) and asymptotic or bilateral significance. Results: 70 students were analyzed during two different measurement periods. According to the Fonseca Index, initially, 37.14% of the analyzed students showed mild TMDs, 17.14% moderate TMDs, and 45.72% showed no TMDs. In general terms, stress increased with age and is related to female sex, maximum mouth opening decreased with age, oxygen saturation decreased with age and academic stress, and myoglobin concentration was related to age. Furthermore, muscle contraction decreased during higher academic stress and increased with age. For women, age was a risk factor for suffering from TMDs, and stress worsened the transition from having TMDs to having no TMDs in both sexes. Conclusions: Academic stress influences TMJ status and muscle outcomes such as oxygen saturation, myoglobin concentration, and muscle contraction, although more research is needed.
Two Different Tumors and Lung Aspergilloma: An Uncommon Etiopathogenic Association
Authors: Ionescu Vlad Alexandru , Gheorghe Gina , Adrian Cosmin , Bebliuc Alexandru , Pavelescu Cezar , Enache Valentin , Gheorghe Florentina , Bacalbasa Nicolae , Diaconu Camelia Cristina
Published: June 2024

Several cases reported in the literature have confirmed the link between pulmonary aspergillosis and various malignant diseases. Furthermore, it has been observed that the correlation between carcinoid tumor and lung adenocarcinoma is quite uncommon. The etiopathogenic mechanisms underlying these correlations remain poorly defined. We present the case of a patient with three of these diseases: a lung adenocarcinoma with a lepidic pattern, a typical carcinoid, and pulmonary aspergillosis. An additional noteworthy aspect of this case pertains to the timely detection of both lung malignancies. Thus, the necessity for further investigation to ascertain the pathogenic connection among the three diseases is underscored. The ultimate objective is to enhance the prognosis of individuals diagnosed with lung cancer, which is a prevailing malignant disease on a global scale.
Optimization of Mechanical Properties of High-Manganese Steel for LNG Storage Tanks: A Comprehensive Review of Alloying Element Effects
Authors: Li Yuchen , Li Jiguang , Zhang Dazheng , Pang Qihang
Published: June 2024

High-manganese austenitic steel represents an innovative variety of low-temperature steel used in the construction of liquefied natural gas (LNG) storage tanks. This steel boasts remarkable characteristics such as exceptional plasticity, superior toughness at cryogenic temperatures, and robust fatigue resistance, all while providing significant cost benefits. By utilizing high-manganese steel, the material manufacturing costs can be considerably lowered, simultaneously ensuring the long-term stability and safety of LNG storage tanks. The alloying design is pivotal in attaining superior performance in high-manganese steel. Choosing the right chemical components to control the stacked fault energy (SFE) of high-manganese steel and fine-tuning its structure can further improve the balance between strength and plasticity. Summarizing the advancements in alloying design for high-manganese steel is of great importance, as it offers a foundational dataset for correlating the chemical composition with the performance. Therefore, this paper outlines the deformation mechanisms and the principles of low-temperature brittleness in high-manganese austenitic steel, and from this foundation, it explicates the precise functions of alloying elements within it. This aims to provide a reference for future alloying designs and the industrial deployment of high-manganese steel in LNG storage tanks.
Mechanical Properties and Interfacial Characterization of Additive-Manufactured CuZrCr/CoCrMo Multi-Metals Fabricated by Powder Bed Fusion Using Pulsed Wave Laser
Authors: Zhang Hao , Jin Xiang , Xiao Zhongmin , Yao Liming
Published: June 2024

In this study, CoCrMo cuboid samples were deposited on a CuZrCr substrate using laser powder bed fusion (L-PBF) technology to investigate the influence of process parameters and laser remelting strategies on the mechanical properties and interface characteristics of multi-metals. This study found that process parameters and laser scanning strategies had a significant influence on the mechanical properties and interface characteristics. Samples fabricated with an EV ≤ 20 J/mm3 showed little tensile ductility. As the volumetric energy density (EV) increased to a range between 40 J/mm3 and 100 J/mm3, the samples achieved the desired mechanical properties, with a strong interface combining the alloys. However, an excessive energy density could result in cracks due to thermal stress. Laser remelting significantly improved the interface properties, especially when the EV was below 40 J/mm3. Variances in the EV showed little influence on the hardness at the CuZrCr end, while the hardness at the interface and the CoCrMo end showed an increasing and decreasing trend with an increase in the EV, respectively. Interface characterization showed that when the EV was greater than 43 J/mm3, the main defects in the L-PBF CoCrMo samples were thermal cracks, which gradually changed to pores with a lack of fusion when the EV decreased. This study provides theoretical and technical support for the manufacturing of multi-metal parts using L-PBF technology.
Efficacy of Lasmiditan as a Secondary Treatment for Migraine Attacks after Unsuccessful Treatment with a Triptan
Authors: Shibata Yasushi , Sato Hiroshige , Sato Akiko , Harada Yoichi
Published: June 2024

The combined use of lasmiditan and triptan is unexplored in medical literature. This study aimed to investigate whether the intake of lasmiditan following triptan improves migraine pain. Following triptan intake, if headache relief was less than 50% at 1 h, patients took 50 mg of lasmiditan within 2 h of migraine onset. Patients recorded headache intensity and adverse events (AEs) caused by lasmiditan at 1, 2, and 4 h after the intake of an additional 50 mg of lasmiditan. A significant reduction in pain scale was observed post 50 mg lasmiditan intake (p < 0.001, t-test). Pain relief was reported for 32 migraine attacks (80%) at 1 h after additional lasmiditan intake. Although AEs were observed in 63% of the patients who took an additional lasmiditan, most were mild and resolved 1 h after lasmiditan intake. Our study revealed the significant headache relief provided by an additional lasmiditan for patients who did not achieve satisfactory results following initial triptan intake for treating migraine. The AEs associated with this treatment strategy were mild and lasted for a short time. This study suggested that the combination of triptan and lasmiditan is promising for the treatment of migraine and should be studied in a randomized placebo-controlled trial.
Development and Application of an In Vitro Tick Feeding System to Identify Ixodes Tick Environment-Induced Genes of the Lyme Disease Agent, Borrelia burgdorferi
Authors: Yamasaki Youki , Singh Preeti , Vimonish Rubikah , Ueti Massaro , Bankhead Troy
Published: June 2024

The bacterial agent of Lyme disease, Borrelia burgdorferi, exists in an enzootic cycle by adapting to dissimilar mammalian and tick environments. The genetic elements necessary for host and vector adaptation are spread across a bacterial genome comprised of a linear chromosome and essential linear and circular plasmids. The promoter trap system, In Vivo Expression Technology (IVET), has been used to identify promoters of B. burgdorferi that are transcriptionally active specifically during infection of a murine host. However, an observed infection bottleneck effect in mice prevented the application of this system to study promoters induced in a tick environment. In this study, we adapted a membrane-based in vitro feeding system as a novel method to infect the Ixodes spp. vector with B. burgdorferi. Once adapted, we performed IVET screens as a proof of principle via an infected bloodmeal on the system. The screen yielded B. burgdorferi promoters that are induced during tick infection and verified relative expression levels using qRT-PCR. The results of our study demonstrate the potential of our developed in vitro tick feeding system and IVET systems to gain insight into the adaptive gene expression of the Lyme disease bacteria to the tick vector.
Design of Experiment (DoE) Approach for Developing Inhalable PLGA Microparticles Loaded with Clofazimine for Tuberculosis Treatment
Authors: Rongala Druva Sarika , Patil Suyash M. , Kunda Nitesh K.
Published: June 2024

Tuberculosis (TB) is an airborne bacterial infection caused by Mycobacterium tuberculosis (M. tb), resulting in approximately 1.3 million deaths in 2022 worldwide. Oral therapy with anti-TB drugs often fails to achieve therapeutic concentrations at the primary infection site (lungs). In this study, we developed a dry powder inhalable formulation (DPI) of clofazimine (CFZ) to provide localized drug delivery and minimize systemic adverse effects. Poly (lactic acid-co-glycolic acid) (PLGA) microparticles (MPs) containing CFZ were developed through a single emulsion solvent evaporation technique. Clofazimine microparticles (CFZ MPs) displayed entrapment efficiency and drug loading of 66.40 ± 2.22 %w/w and 33.06 ± 1.45 µg/mg, respectively. To facilitate pulmonary administration, MPs suspension was spray-dried to yield a dry powder formulation (CFZ SD MPs). Spray drying had no influence on particle size (~1 µm), zeta potential (−31.42 mV), and entrapment efficiency. Solid state analysis (PXRD and DSC) of CFZ SD MPs studies demonstrated encapsulation of the drug in the polymer. The drug release studies showed a sustained drug release. The optimized formulation exhibited excellent aerosolization properties, suggesting effective deposition in the deeper lung region. The in vitro antibacterial studies against H37Ra revealed improved (eight-fold) efficacy of spray-dried formulation in comparison to free drug. Hence, clofazimine dry powder formulation presents immense potential for the treatment of tuberculosis with localized pulmonary delivery and improved patient compliance.
Characterization and Comparison of Contrast Imaging Properties of Naturally Isolated and Heterologously Expressed Gas Vesicles
Authors: Liu Tingting , Wang Jieqiong , Liu Chenxing , Wang Yuanyuan , Li Zhenzhou , Yan Fei
Published: June 2024

Nanoscale ultrasound contrast agents have attracted considerable interest in the medical imaging field for their ability to penetrate tumor vasculature and enable targeted imaging of cancer cells by attaching to tumor-specific ligands. Despite their potential, traditional chemically synthesized contrast agents face challenges related to complex synthesis, poor biocompatibility, and inconsistent imaging due to non-uniform particle sizes. To address these limitations, bio-synthesized nanoscale ultrasound contrast agents have been proposed as a viable alternative, offering advantages such as enhanced biocompatibility, consistent particle size for reliable imaging, and the potential for precise functionalization to improve tumor targeting. In this study, we successfully isolated cylindrical gas vesicles (GVs) from Serratia. 39006 and subsequently introduced the GVs-encoding gene cluster into Escherichia coli using genetic engineering techniques. We then characterized the contrast imaging properties of two kinds of purified GVs, using in vitro and in vivo methods. Our results demonstrated that naturally isolated GVs could produce stable ultrasound contrast signals in murine livers and tumors using clinical diagnostic ultrasound equipment. Additionally, heterologously expressed GVs from gene-engineered bacteria also exhibited good ultrasound contrast performance. Thus, our study presents favorable support for the application of genetic engineering techniques in the modification of gas vesicles for future biomedical practice.
Predicting ADMET Properties from Molecule SMILE: A Bottom-Up Approach Using Attention-Based Graph Neural Networks
Authors: De Carlo Alessandro , Ronchi Davide , Piastra Marco , Tosca Elena Maria , Magni Paolo
Published: June 2024

Understanding the pharmacokinetics, safety and efficacy of candidate drugs is crucial for their success. One key aspect is the characterization of absorption, distribution, metabolism, excretion and toxicity (ADMET) properties, which require early assessment in the drug discovery and development process. This study aims to present an innovative approach for predicting ADMET properties using attention-based graph neural networks (GNNs). The model utilizes a graph-based representation of molecules directly derived from Simplified Molecular Input Line Entry System (SMILE) notation. Information is processed sequentially, from substructures to the whole molecule, employing a bottom-up approach. The developed GNN is tested and compared with existing approaches using six benchmark datasets and by encompassing regression (lipophilicity and aqueous solubility) and classification (CYP2C9, CYP2C19, CYP2D6 and CYP3A4 inhibition) tasks. Results show the effectiveness of our model, which bypasses the computationally expensive retrieval and selection of molecular descriptors. This approach provides a valuable tool for high-throughput screening, facilitating early assessment of ADMET properties and enhancing the likelihood of drug success in the development pipeline.
Recent Advances in the Therapeutic Potential of Carotenoids in Preventing and Managing Metabolic Disorders
Authors: Ortega-Regules Ana E. , Martínez-Thomas Juan Alonso , Schürenkämper-Carrillo Karen , de Parrodi Cecilia Anaya , López-Mena Edgar R. , Mejía-Méndez Jorge L. , Lozada-Ramírez J. Daniel
Published: June 2024

Carotenoids constitute compounds of significant biological interest due to their multiple biological activities, such as antimicrobial, anticancer, antiadipogenic, antidiabetic, and antioxidant properties. Metabolic syndrome (MetS) comprehends a series of metabolic abnormalities (e.g., hypertension, obesity, and atherogenic dyslipidemia) that can affect children, adolescents, and the elderly. The treatment of MetS involves numerous medications, which, despite their efficacy, pose challenges due to prolonged use, high costs, and various side effects. Carotenoids and their derivatives have been proposed as alternative treatments to MetS because they reduce serum triglyceride concentrations, promote insulin response, inhibit adipogenesis, and downregulate angiotensin-converting enzyme activity. However, carotenoids are notably sensitive to pH, light exposure, and temperature. This review addresses the activity of carotenoids such as lycopene, lutein, fucoxanthin, astaxanthin, crocin, and β-carotene towards MetS. It includes a discussion of sources, extraction methods, and characterization techniques for analyzing carotenoids. Encapsulation approaches are critically reviewed as alternatives to prevent degradation and improve the biological performance of carotenoids. A brief overview of the physiopathology and epidemiology of the diseases, including MetS, is also provided.
Plant Leaf Functional Adaptions along Urban–Rural Gradients of Jinhua City
Authors: Cao Chenchen , Cui Shufen , Guan Xinyu , Chen Yuanjian , Zhang Yongqi , Lin Xingwen , Wu Chaofan , Zhang Zhaoyang , Zhang Fei , Xu Yuling , Zhang Zhenzhen
Published: June 2024

Environmental changes induced by urbanization may significantly alter plant survival strategies, thereby introducing uncertainties in their ability to withstand extreme heat. This study, centered on Jinhua City, distinguished urban, suburban, and rural areas to represent the various intensities of urbanization. It examined the leaf function properties of evergreen and deciduous trees common in these regions, focusing on leaf and branch characteristics. Employing an analysis of variance (ANOVA), principal component analysis (PCA), and path analysis (PA) of the plant functional traits and the climatic factors of each region, this study assessed the impact of urbanization on plant survival strategies. By tracking changes in plant functional traits from June to August, it explored the capacity of plants to acclimate to urban-warming-related heat stress across different urbanization gradients. The findings revealed that leaf thickness (LT) and stomatal size (SS) initially decreased and then increased, whereas specific leaf area (SLA) and leaf tissue density (LTD) first rose and then declined, from rural to urban regions. From June to August, branch wood density (WD), chlorophyll (Chl) content, LTD, and leaf dry matter content (LDMC) increased, whereas SLA and leaf water content (LWC) diminished, in all regions. PCA suggested that there was no significant change in the resource allocation strategy of plants (p > 0.05), with drought tolerance significantly reduced in the suburbs on the gradient of urbanization (p < 0.05). During the summer, with high temperature, plants were predominantly biased towards slow-return, conservative strategies, particularly among evergreen species. Compared to precipitation, PA revealed a significant urban warming effect. During summer, temperature was the main factor influencing resource investment strategy and drought resistance, with a notably stronger impact on the former. The high temperature in summer promoted a conservative survival strategy in plants, and the urbanization effect increased their tolerance to high temperatures.