bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy and Safety of Lenvatinib Combined With PD-1 Inhibitors Plus TACE for Unresectable Hepatocellular Carcinoma Patients in China Real-World
Authors: Li Xiaowei , Fu Zhigang , Chen Xiaoxia , Cao Kunkun , Zhong Jiaming , Liu Li , Ding Ning , Zhang Xiaoli , Zhai Jian , Qu Zengqiang
Published: July 2022

Purpose

To evaluate the efficacy and safety of lenvatinib combined with programmed death receptor-1 signaling inhibitors plus transarterial chemoembolization (LePD1-TACE) for treatment of unresectable hepatocellular carcinoma (uHCC) in a real-world setting in China.

Methods

This was a retrospective study involving consecutive patients with uHCC (n =114) receiving LePD1-TACE treatment from June 2019 to May 2021. Overall survival (OS), progression-free survival (PFS), objective response rate (ORR), and disease control rate (DCR) were calculated to evaluate the antitumor efficacy. Treatment-related adverse events (TRAEs) were analyzed to assess the safety profiles. In addition, we also evaluated prognostic factors related to survival and disease progression.

Results

A total of 114 patients with a median age of 53 years were analyzed during a median follow-up duration of 10.6 months (95% confidence interval [CI]: 8.5 -12.8). The Kaplan-Meier analysis showed that the median OS was 18.0 months (95% CI: 14.1 - Not reached), the median PFS was 10.4 months (95% CI: 6.6 - 12.4). Based on modified Response Evaluation Criteria in Solid Tumors, the best ORR was 69.3% and DCR was 80.7%. Almost all patients suffered from TRAEs, the most common grade 3-4 TRAEs were hypertension (8.8%), proteinuria (3.6%), hyperbilirubinemia (1.8%), leukopenia (4.4%) and alanine aminotransferase elevation (3.6%) across all patients. The independent treatment factors associated with OS and PFS were tumor number, neutrophil-to-lymphocyte ratio (NLR) and the early tumor response. In the early tumor response (CR+PR) patients, median OS and PFS were 25.1 months (95% CI: 13.8 - Not reached) and 15.2 months (95% CI: 10.5 - 19.1). The patients with tumor number < 3 had a superior median OS and PFS (25.1, 16.4 months) compared to patients with tumor number ≥ 3 (14.1 months, P = 0.012; 6.6 months, P = 0.007). The patients with NLR ≤ 2.165 had a longer median OS and PFS (Not reached, 15.2 months) than those with NLR > 2.165 (17.7 months, P = 0.003; 7.5 months, P = 0.047).

Conclusion

In this real-world study, LePD1-TACE triple therapy showed encouraging efficiency and manageable safety in patients with uHCC. The tumor number (< 3), NLR (≤ 2.165) and early tumor response (CR+PR) could be one of the prognostic markers.
Implementation Science in Pediatric Critical Care – Sedation and Analgesia Practices as a Case Study
Authors: Yang Youyang , Geva Alon , Madden Kate , Mehta Nilesh M.
Published: July 2022

Sedation and analgesia (SA) management is essential practice in the pediatric intensive care unit (PICU). Over the past decade, there has been significant interest in optimal SA management strategy, due to reports of the adverse effects of SA medications and their relationship to ICU delirium. We reviewed 13 studies examining SA practices in the PICU over the past decade for the purposes of reporting the study design, outcomes of interest, SA protocols used, strategies for implementation, and the patient-centered outcomes. We highlighted the paucity of evidence-base for these practices and also described the existing gaps in the intersection of implementation science (IS) and SA protocols in the PICU. Future studies would benefit from a focus on effective implementation strategies to introduce and sustain evidence-based SA protocols, as well as novel quasi-experimental study designs that will help determine their impact on relevant clinical outcomes, such as the occurrence of ICU delirium. Adoption of the available evidence-based practices into routine care in the PICU remains challenging. Using SA practice as an example, we illustrated the need for a structured approach to the implementation science in pediatric critical care. Key components of the successful adoption of evidence-based best practice include the assessment of the local context, both resources and barriers, followed by a context-specific strategy for implementation and a focus on sustainability and integration of the practice into the permanent workflow.
Epimedin C Alleviates Glucocorticoid-Induced Suppression of Osteogenic Differentiation by Modulating PI3K/AKT/RUNX2 Signaling Pathway
Authors: Xu Yongxiang , Chen Shichun , Huang Linxuan , Han Weichao , Shao Yingying , Chen Minyi , Zhang Yusheng , He Ruirong , Xie Baocheng
Published: July 2022

Secondary osteoporosis is triggered mostly by glucocorticoid (GC) therapy. Dexamethasone (DEX) was reported to inhibit osteogenic differentiation in zebrafish larvae and MC3T3-E1 cells in prior research. In this research, we primarily examined the protective impacts of epimedin C on the osteogenic inhibition impact of MC3T3-E1 cells and zebrafish larvae mediated by DEX. The findings illustrated no apparent toxicity for MC3T3-E1 cells after administering epimedin C at increasing dosages from 1 to 60 μM and no remarkable proliferation in MC3T3-E1 cells treated using DEX. In MC3T3-E1 cells that had been treated using DEX, we discovered that epimedin C enhanced alkaline phosphatase activities and mineralization. Epimedin C could substantially enhance the protein expression of osterix (OSX), Runt-related transcription factor 2 (RUNX2), and alkaline phosphatase (ALPL) in MC3T3-E1 cells subjected to DEX treatment. Additionally, epimedin C stimulated PI3K and AKT signaling pathways in MC3T3-E1 cells that had been treated using DEX. Furthermore, in a zebrafish larvae model, epimedin C was shown to enhance bone mineralization in DEX-mediated bone impairment. We also found that epimedin C enhanced ALPL activity and mineralization in MC3T3-E1 cells treated using DEX, which may be reversed by PI3K inhibitor (LY294002). LY294002 can also reverse the protective impact of epimedin C on DEX-mediated bone impairment in zebrafish larval. These findings suggested that epimedin C alleviated the suppressive impact of DEX on the osteogenesis of zebrafish larval and MC3T3-E1 cells via triggering the PI3K and AKT signaling pathways. Epimedin C has significant potential in the development of innovative drugs for the treatment of glucocorticoid-mediated osteoporosis.
The Function of Natural Polysaccharides in the Treatment of Ulcerative Colitis
Authors: Guo Yafei , Li Yang , Cao Qiang , Ye Leilei , Wang Junmei , Guo Mei
Published: July 2022

Ulcerative colitis (UC) is an inflammatory bowel disease that is persistent and nonspecific. There are several medications available for the treatment of UC. However, conventional UC medications have substantial adverse effects, low clinical effectiveness, and a high recurrence rate. Therefore, it is critical to discover new medicines that are both safe and effective for UC patients. Natural polysaccharides offer a wide range of pharmacological benefits, including anti-inflammatory, anti-virus, anti-tumor, anti-aging, immune enhancement, and gut flora regulation. In the therapy of UC, natural polysaccharides can modulate inflammatory factors, the immune system, and intestinal flora, and preserve the intestinal mucosa. It demonstrates a good curative effect and is of safety to use, thereby being a potential treatment for UC patients. This paper covers the structure, the pharmacological effects on UC, and the mechanisms of natural polysaccharides. Finally, limitations, challenges, and perspectives are discussed. It is hoped that the findings of this publication will inspire more natural polysaccharides research and provide a theoretical foundation for the creation of new UC medications.
Entangled Biphoton Enhanced Double Quantum Coherence Signal as a Probe for Cavity Polariton Correlations in Presence of Phonon Induced Dephasing
Authors: Debnath Arunangshu , Rubio Angel
Published: July 2022

We theoretically propose a biphoton entanglement-enhanced multidimensional spectroscopic technique as a probe for the dissipative polariton dynamics in the ultrafast regime. It is applied to the cavity-confined monomeric photosynthetic complex that represents a prototypical multi-site excitonic quantum aggregate. The proposed technique is shown to be particularly sensitive to inter-manifold polariton coherence between the two and one-excitation subspaces. It is demonstrated to be able to monitor the dynamical role of cavity-mediated excitonic correlations, and dephasing in the presence of phonon-induced dissipation. The non-classicality of the entangled biphoton sources is shown to enhance the ultra-fast and broadband correlation features of the signal, giving an indication about the underlying state correlations responsible for long-range cavity-assisted exciton migration.
Femtosecond Third-Order Non-Linear Optical Properties of Unconstrained Green Fluorescence Protein Chromophores
Authors: Ahmed Md Soif , Biswas Chinmoy , Banerjee Dipanjan , Chetti Prabhakar , Yang Jye-Shane , Soma Venugopal Rao , Raavi Sai Santosh Kumar
Published: July 2022

We report herein results on the third-order non-linear optical (NLO) properties of four structurally unconstrained green fluorescence protein (GFP) chromophores, namely, 1, 2, 3, and 4. Using experimental techniques and theoretical calculations such as UV–visible spectroscopy, density functional theory (DFT), time-dependent density functional theory (TDDFT), and Z-scan techniques, we have investigated the linear absorption, ultrafast non-resonant third-order optical non-linearities, and the onset of optical-limiting thresholds of these benzylidenedimethylimidazolinone (BDI) dyes. The Z-scan measurements were performed at a wavelength of 800 nm with ∼70 femtosecond (fs) pulses. We have witnessed a strong reverse saturable absorption (fitted to three-photon absorption) for all of the molecules with fs pulse excitation. The valley–peak curves obtained from the closed-aperture Z-scan technique revealed the positive non-linear refractive index (self-focusing) nature of these molecules. We have evaluated the various third-order NLO coefficients (second hyperpolarizability, γ ∼10–33 esu), which were found to be larger than those of similar molecules reported in the recent literature.
Femtosecond Laser Fabrication of Noble Metal Plasma Nanostructures and Its Application-A Mini Review
Authors: Feng Yanshuo , Liang Misheng , You Rui , Li Tianshu , Zhu Lianqing
Published: July 2022

Noble metal plasmonic nanostructures have a wide range of applications in surface-enhanced Raman scattering (SERS), solar energy conversion, optical imaging, biosensing, and many other fields due to their unique localized surface plasmon resonance effects and optical properties. In recent years, the fabrication method of noble metal plasmonic nanostructures has attracted a lot of research interest. Compared with traditional processing methods, femtosecond laser processing has the advantages of high processing resolution, small heat-affected zone, high processing flexibility, controllability, and ease of patterning, and has shown great potential for application in the fabrication method of noble metal plasmonic nanostructures. In this paper, the recent advances in the fabrication of noble metal plasmonic nanostructures by femtosecond laser are reviewed, followed by a discussion of the characteristics, advantages, and potential applications of the structures prepared by this method.
Percutaneous Auricular Vagus Nerve Stimulation Reduces Inflammation in Critical Covid-19 Patients
Authors: Seitz Tamara , Szeles József Constantin , Kitzberger Reinhard , Holbik Johannes , Grieb Alexander , Wolf Hermann , Akyaman Hüseyin , Lucny Felix , Tychera Alexander , Neuhold Stephanie , Zoufaly Alexander , Wenisch Christoph , Kaniusas Eugenijus
Published: July 2022

Covid-19 is an infectious disease associated with cytokine storms and derailed sympatho-vagal balance leading to respiratory distress, hypoxemia and cardiovascular damage. We applied the auricular vagus nerve stimulation to modulate the parasympathetic nervous system, activate the associated anti-inflammatory pathways, and reestablish the abnormal sympatho-vagal balance. aVNS is performed percutaneously using miniature needle electrodes in ear regions innervated by the auricular vagus nerve. In terms of a randomized prospective study, chronic aVNS is started in critical, but not yet ventilated Covid-19 patients during their stay at the intensive care unit. The results show decreased pro-inflammatory parameters, e.g. a reduction of CRP levels by 32% after 1 day of aVNS and 80% over 7 days (from the mean 151.9 mg/dl to 31.5 mg/dl) or similarly a reduction of TNFalpha levels by 58.1% over 7 days (from a mean 19.3 pg/ml to 8.1 pg/ml) and coagulation parameters, e.g. reduction of DDIMER levels by 66% over 7 days (from a mean 4.5 μg/ml to 1.5 μg/ml) and increased anti-inflammatory parameters, e.g. an increase of IL-10 levels by 66% over 7 days (from the mean 2.7 pg/ml to 7 pg/ml) over the aVNS duration without collateral effects. aVNS proved to be a safe clinical procedure and could effectively supplement treatment of critical Covid-19 patients while preventing devastating over-inflammation.
Vicariance Between Cercis siliquastrum L. and Ceratonia siliqua L. Unveiled by the Physical–Chemical Properties of the Leaves’ Epicuticular Waxes
Authors: Pereira Rui F. P. , Rocha João , Nunes Paulo , Fernandes Tânia , Ravishankar Ajith P. , Cruz Rebeca , Fernandes Mariana , Anand Srinivasan , Casal Susana , de Zea Bermudez Verónica , Crespí António L.
Published: July 2022

Classically, vicariant phenomena have been essentially identified on the basis of biogeographical and ecological data. Here, we report unequivocal evidences that demonstrate that a physical–chemical characterization of the epicuticular waxes of the surface of plant leaves represents a very powerful strategy to get rich insight into vicariant events. We found vicariant similarity between Cercis siliquastrum L. (family Fabaceae, subfamily Cercidoideae) and Ceratonia siliqua L. (family Fabaceae, subfamily Caesalpinoideae). Both taxa converge in the Mediterranean basin (C. siliquastrum on the north and C. siliqua across the south), in similar habitats (sclerophyll communities of maquis) and climatic profiles. These species are the current representation of their subfamilies in the Mediterranean basin, where they overlap. Because of this biogeographic and ecological similarity, the environmental pattern of both taxa was found to be very significant. The physical–chemical analysis performed on the epicuticular waxes of C. siliquastrum and C. siliqua leaves provided relevant data that confirm the functional proximity between them. A striking resemblance was found in the epicuticular waxes of the abaxial surfaces of C. siliquastrum and C. siliqua leaves in terms of the dominant chemical compounds (1-triacontanol (C30) and 1-octacosanol (C28), respectively), morphology (intricate network of randomly organized nanometer-thick and micrometer-long plates), wettability (superhydrophobic character, with water contact angle values of 167.5 ± 0.5° and 162 ± 3°, respectively), and optical properties (in both species the light reflectance/absorptance of the abaxial surface is significantly higher/lower than that of the adaxial surface, but the overall trend in reflectance is qualitatively similar). These results enable us to include for the first time C. siliqua in the vicariant process exhibited by C. canadensis L., C. griffithii L., and C. siliquastrum.
Glutathione Promotes Degradation and Metabolism of Residual Fungicides by Inducing UDP-Glycosyltransferase Genes in Tomato
Authors: Yu Gaobo , Chen Qiusen , Chen Fengqiong , Liu Hanlin , Lin Jiaxin , Chen Runan , Ren Chunyuan , Wei Jinpeng , Zhang Yuxian , Yang Fengjun , Sheng Yunyan
Published: July 2022

Reduced glutathione (GSH) is a key antioxidant, which plays a crucial role in the detoxification of xenobiotics in plants. In the present study, glutathione could reduce chlorothalonil (CHT) residues in tomatoes by inducing the expression of the UDP-glycosyltransferase (UGT) gene. In plants, UGT is an important glycosylation catalyst, which can respond to stresses in time by activating plant hormones and defense compounds. Given the importance of plant growth and development, the genome-wipe analyses of Arabidopsis and soybean samples have been carried out, though not on the tomato, which is a vital vegetable crop. In this study, we identified 143 UGT genes in the tomato that were unevenly distributed on 12 chromosomes and divided into 16 subgroups and found that a variety of plant hormones and stress response cis-elements were discovered in the promoter region of the SlUGT genes, indicating that the UGT genes were involved in several aspects of the tomato stress response. Transcriptome analysis and results of qRT-PCR showed that most SlUGT genes could be induced by CHT, and the expression of these genes was regulated by glutathione. In addition, we found that SlUGT genes could participate in plant detoxification through interaction with transcription factors. These findings further clarify the potential function of the UGT gene family in the detoxification of exogenous substances in tomatoes and provide valuable information for the future study of functional genomics of tomatoes.
Financial Crisis in Management Stress: From the Perspective of Crisis Anxiety of Others
Authors: Liu Bin , Zhu Jing , Su Fangguo , Wen Bin , Wu Yingqi
Published: July 2022

The crisis anxiety of others is a phenomenon that goes hand in hand with the spread of the occupational health pandemic. It is becoming increasingly important to better understand its emergence process, especially in the era of greater uncertainty. This study aims to examine the impact of the external financial crisis on managerial stress among financial employees. The sample consists of 347 senior managers and financial employees from companies in China. The empirical analysis shows that external financial crises have significant effects on anxiety levels, especially external corporate crisis, debt crisis and growth crisis both have mediating effect on the relationship between anxiety level and pressure management and the relationship between external financial crisis and pressure management. This study explores the rules for the emergence of anxiety among corporate managers and expands the scope of environmental factors that need to be discussed in the study of corporate financial management. This study provides theoretical implications for the psychological study of Financial Management and practical implications for corporate financial management.
Two Morphological Awareness Components Have Different Roles in Chinese Word Reading Development for Primary Schoolers
Authors: Liu Hailun , Wang Jing , Wang Lei , Zhang Wenjun , Deng Ciping
Published: July 2022

Morphological awareness is multi-factorial by nature and consists of general morphological knowledge and morphological meaning analysis; the first refers to the recognition and manipulation of morphological structures, and the second refers to the inference of word semantics by utilizing morphological knowledge. Contrasting the roles of two morphological awareness components in word reading could help resolve the controversy about whether morphological awareness could independently contribute to Chinese word reading. Thus, the study explored how morphological awareness components contributed to word reading development in Chinese context. A group of 299 Chinese children in grades 3 and 4 were recruited and tested twice with the interval of half a year, by a series of tasks on morphological awareness components, word reading, and some control variables. Results showed that, after controlling for vocabulary and other linguistic variables, morphological meaning analysis could independently predict word reading, whereas general morphological knowledge could only indirectly predict word reading, a process mediated by morphological meaning analysis. This study showed independent contribution of morphological awareness to Chinese word reading development. By clarifying the ways of how different morphological awareness components support children’s word reading development, the findings enhance our understanding about the potential mechanism underlying the relation between morphological awareness and Chinese word reading.
Anticipatory and Anticipated Emotions in Regular and Non-regular Exercisers – A Qualitative Study
Authors: Feil Katharina , Weyland Susanne , Fritsch Julian , Wäsche Hagen , Jekauc Darko
Published: July 2022

Future-oriented emotions could influence our decisions in everyday life and help understand why some individuals are physically active whilst others are not. Current literature distinguishes between two future-oriented emotion constructs: anticipatory and anticipated emotions. While anticipatory emotions are currently experienced emotions about a future event, anticipated emotions refer to the emotions that a person is expected to experience when confronted with a future event. The main aims of the present study were (1) to identify and describe (a) categories of anticipatory emotions experienced before exercise, and (b) categories of anticipated emotions expected to be experienced during and after exercise, and (2) to develop a theoretical model of anticipated emotion categories. Sixteen participants (Mage = 26.03, SD = 6.66) were recruited for semi-structured interviews, and their statements were analyzed using principles of the Grounded Theory. In total, 13 different anticipatory and anticipated emotion categories were identified, such as enjoyment, anxiety, pride, self-anger, and relief. Anticipatory emotions seem to reflect the current affective valence of exercising and may be influenced by daily factors. With regards to anticipated emotions, the results show that regular exercisers anticipated also negative emotions such as anxiety, disappointment, and self-anger, and non-regular exercisers also anticipated positive emotions such as enjoyment, pride, and satisfaction. Therefore, future research should not only focus on the valence of future-oriented emotions, but should investigate the possible impact of specific anticipated emotions on exercise behavior. In addition, a theoretical model of anticipated emotion categories in exercise behavior derived from the interviews. The model outlines different categories of anticipated emotions based on appraisal processes. In conclusion, we assume that this developmental process of anticipated emotions may be embedded in a broader, cyclical process within the context of exercising.
Being Tired or Having Much Left Undone: The Relationship Between Fatigue and Unfinished Tasks With Affective Rumination and Vitality in Beginning Teachers
Authors: Weiher Gerald M. , Varol Yasemin Z. , Horz Holger
Published: July 2022

The present diary study was conducted for the purpose of bridging and integrating empirical research on the antecedents and consequences of work-related ruminative processes in the evening. Based on the control theory, unfinished tasks and fatigue in the afternoon were considered as antecedents of affective rumination, while vitality was investigated as the outcome observed in the next morning to test for cyclical processes. During a 5-day diary study (including 3 weekdays and the weekend), 74 beginning teachers completed three diary entries per day. A total of 795 diary entries were obtained. Using multilevel structural equation modeling, the study supported that both fatigue and unfinished tasks explained unique shares of variance of affective rumination in the evening at the between- and within-person levels. Furthermore, affective rumination mediated the relationship between unfinished tasks and vitality as well as fatigue and vitality. However, this only held true at the between- and not the within-person level, as neither affective rumination nor fatigue and unfinished tasks predicted the following morning’s vitality at this level. The results offer insights into the antecedents of affective rumination and add to extant research on the negative consequences of affective rumination considering vitality as an outcome.
The Relationship Among Financial Inclusion, Non-Performing Loans, and Economic Growth: Insights From OECD Countries
Authors: Zhang Peng , Zhang Mengxue , Zhou Qiancheng , Zaidi Syed Anees Haider
Published: July 2022

This study implies finding the linkages among financial inclusion, non-performing loans (NPLs), and economic growth. The study uses large panel data of 21 Organization for Economic Corporation and Development (OECD) countries for the dynamic panel estimation by using the Driscoll-Kraay standard errors with fixed effect. The results of the dynamic panel estimation technique revealed the existence of a long-run relationship among financial inclusion, NPLs, and economic growth. Financial inclusion contributes positively to economic growth by reducing NPLs. Furthermore, NPLs negatively impact financial inclusion as well as economic growth. The study presents important policy recommendations to control NPLs and boost the level of financial inclusion in the selected economies.
Integration and Causality in Enactive Approaches to Psychiatry
Authors: Gallagher Shaun
Published: July 2022

In this paper I address what has been called the integration problem in psychiatry. This problem is tied to conceptions of causality and explanatory levels in our understanding of mind. I take an interdisciplinary enactive perspective to develop a 3-fold method for exploring the dynamics of integration, based on a concept of dynamical causation and a non-hierarchical (level-free) notion of gestalt. I also consider Autism Spectrum Disorder (ASD) as a test case.
Resting Heart Rate Variability, Perceived Emotion Regulation, and Low-Risk Drug Use in College-Aged Adults: Gender as a Moderator
Authors: Kwon Enoch S. , Kittaneh Ahmad A. , Gerardo Gina M. , Koenig Julian , Thayer Julian F. , Williams DeWayne P.
Published: July 2022

Identification of individual differences in drug use is warranted, as a history of use is associated with future drug problems. Such drug use is thought to disrupt inhibitory and motivation networks involved in emotion regulation (ER). Higher resting heart rate variability (HRV), a biomarker of effective inhibitory abilities, is associated with less substance (e.g., alcohol, opioid) use. Higher HRV is associated with lower perceived ER difficulties, and this link is stronger in women relative to men. Evidence suggests women might engage in drug use primarily to reduce stress, and men primarily to induce feelings of elation. Research has yet to examine associations among individuals’ difficulties in ER, resting HRV, and a recent history of drug use; the current study explored this, in addition to how these associations might differ as a function of gender. Young and healthy college students (N = 190; 88 women) completed a 5-min baseline to assess resting HRV, followed by the 36-item difficulties in ER Scale and 10-item Drug Abuse Screening Test. Higher difficulties in ER, but not resting HRV, were associated with a greater history of “low-risk” drug use in the full sample and moderation tests confirm this link was stronger in women. Moderated-mediation results confirmed an indirect association between resting HRV and drug use, mediated by self-reported difficulties among women only. A significant association between resting HRV and Difficulties in Emotion Regulation Scale (DERS) emerged only among women without a history of drug use. These results indicate that difficulties in ER are both associated with a low-risk history of drug use and underlie an indirect link between resting HRV and drug use history in women only. Among these women with a history of drug use relative to women without, there was no link between resting HRV and self-reported difficulties in ER, suggesting a disrupted inhibitory-motivational pathway. Additional work is needed to understand the psychophysiological correlates of a history of low-risk drug use in young men. These data are in line with research suggesting gender differences in the motivation to engage in recreational drug use and ER interventions might be important in women who engage in low-risk recreational drug use.
Regional Differences in Carbon-14 Data of the 993 CE Cosmic Ray Event
Authors: Miyake Fusa , Hakozaki Masataka , Kimura Katsuhiko , Tokanai Fuyuki , Nakamura Toshio , Takeyama Mirei , Moriya Toru
Published: July 2022

Cosmogenic nuclides such as 14C from tree rings and 10Be and 36Cl from ice cores are excellent proxies for the past extremely large solar energetic particle (SEP) events, which are dozens of times larger than the largest SEP event in the history of observation. So far, several rapid 14C increases have been discovered, which are considered to have originated from extreme SEP events (or set of successive SEP events) from verifications using multiple cosmogenic nuclide analyses in natural archives. Although these events are characterized by a rapid increase in cosmogenic nuclide concentrations, 14C data recorded worldwide do not always show similar variations, especially during the 993 CE event, where a rapid increase was recorded in either 992–993 CE or 993–994 CE in several records. We present new 14C data of the Japanese cedar sample for the 993 CE event. Although the latest data show no significant increase in 1 year, an overall increase pattern is consistent with the previously reported 14C data of the Japanese cedar, which supports that a significant 14C increase occurred from 993 to 994 CE in the Japanese sample. Given the dominant 14C production in high latitudes by SEPs, the difference in timing of increase may be a transport effect in the atmosphere. Moreover, the difference in the timing of the 14C increase can cause a 1-year age-determination error using the 993 CE radiocarbon spike. Compared with the 14C data between tree samples from high latitude and midlatitude, including Japan, high-latitude data can capture 14C changes originating from SEP events more quickly and clearly and may be more suitable for a SEP event exploration in the past.
Nutritional and Food Composition Survey of Major Pulses Toward Healthy, Sustainable, and Biofortified Diets
Authors: Langyan Sapna , Yadava Pranjal , Khan Fatima Nazish , Bhardwaj Ragini , Tripathi Kuldeep , Bhardwaj Vikash , Bhardwaj Rakesh , Gautam Raj Kumar , Kumar Ashok
Published: July 2022

The world's food demand is increasing rapidly due to fast population growth that has posed a challenge to meeting the requirements of nutritionally balanced diets. Pulses could play a major role in the human diet to combat these challenges and provide nutritional and physiological benefits. Pulses such as chickpeas, green gram, peas, horse gram, beans, lentils, black gram, etc., are rich sources of protein (190–260 g kg−1), carbohydrates (600–630 g kg−1), dietary fibers, and bioactive compounds. There are many health benefits of phytochemicals present in pulses, like flavonoids, phenolics, tannins, phytates, saponins, lectins, oxalates, phytosterols peptides, and enzyme inhibitors. Some of them have anti-inflammatory, anti-ulcerative, anti-microbial, and anti-cancer effects. Along with these, pulses are also rich in vitamins and minerals. In this review, we highlight the potential role of pulses in global food systems and diets, their nutritional value, health benefits, and prospects for biofortification of major pulses. The food composition databases with respect to pulses, effect of processing techniques, and approaches for improvement of nutritional profile of pulses are elaborated.
Ethical Eggs: Can Synthetic Biology Disrupt the Global Egg Production Industry?
Authors: Mankad Aditi , Hobman Elizabeth V. , Carter Lucy , Tizard Mark
Published: July 2022

Commercial egg production relies on the industry-accepted practice of culling day-old male chicks, which are a live by-product of the egg production industry. Researchers are exploring the use of a transgenic marker gene to allow early identification of male embryos in ovo at the point of lay, rather than upon hatching. Here we examine social acceptability of this biotechnology-enabled solution to sex selection, which addresses the key ethical issue of culling and improved sustainability of food systems. A national online survey (N = 1148) measured psychological factors influencing public support for the development of the technology and willingness to purchase eggs derived from the novel process. Most participants expressed at least a moderate intention to support the development of gene marking technology, with 1 in 5 people expressing strong support. Participants expressed moderate to high agreement that gene marking of chickens would: (a) help reduce or eliminate the practice of culling male chicks in the egg-laying industry (response efficacy), and; (b) that this new synbio approach to sex selection may be better than current methods of identifying and removing male chicks during egg production (relative advantage). Of those participants who consumed eggs, almost 60% reported they would be moderately to strongly willing to purchase eggs derived from the gene marking process. A partially-mediated path model comprising both intention to support and willingness to buy eggs (R2 = 0.78) showed that key factors involved in decision-making, in addition to response efficacy and relative advantage, were evaluative attitudes toward the technology (e.g., was the technology bad/good, risky/safe, unethical/ethical) and emotional reactions. These results suggest that consumers may be primarily basing their decisions and behavioral choices on how valuable they perceive the novel gene marking solution, reflecting on how it compares favorably to current culling practices, yielding a range of benefits such as higher animal welfare, improved sustainability, and reduced waste.
Is It Necessary to Remove the Implants After Fixation of Thoracolumbar and Lumbar Burst Fractures Without Fusion? A Retrospective Cohort Study of Elderly Patients
Authors: Xu Xiangyu , Cao Yuan , Fan JiXing , Lv Yang , Zhou Fang , Tian Yun , Ji Hongquan , Zhang Zhishan , Guo Yan , Yang Zhongwei , Hou Guojin
Published: July 2022

Objective

Fractures of the thoracolumbar spine are the most common fractures of the spinal column. This retrospective cohort study aimed to determine whether it is necessary to remove implants of patients aged over 65 years after the fixation of thoracolumbar and lumbar burst fractures without fusion.

Methods

This retrospective cohort study included 107 consecutive patients aged ≥65 years without neurological deficits, who underwent non-fusion short posterior segmental fixation for thoracolumbar or lumbar burst fractures. Outcome measures included the visual analog score (VAS), Oswestry Disability Index (ODI), residual symptoms, complications, and imaging parameters. Patients were divided into groups A (underwent implant removal) and B (implant retention) and were examined clinically at 1, 3, 6, and 12 months postoperatively and annually thereafter, with a final follow-up at 48.5 months.

Results

Overall, 96 patients with a mean age of 69.4 (range, 65–77) years were analyzed. At the latest follow-up, no significant differences were observed in functional outcomes and radiological parameters between both groups, except in the local motion range (LMR) (P = 0.006). Similarly, between preimplant removal and the latest follow-up in group A, significant differences were found only in LMR (P < 0.001). Two patients experienced screw breakage without clinical symptoms. Significant differences were only found in operation time, blood loss, ODI, and fracture type between minimally invasive group and open group.

Conclusions

Similar radiological and functional outcomes were observed in elderly patients, regardless of implant removal. Implant removal may not be necessary after weighing the risks and benefits for elderly patients. Patients should be informed about the possibility of implant breakage and accelerating degeneration of adjacent segments in advance.
Gastric-type Mucinous Carcinoma with an Abnormal Increase of CA199: A Case Report and Literature Review
Authors: Qian Xue-qian , Wang Fen-fen , Liang Yun , Chen Li-Li , Wan Xiao-yun
Published: July 2022

Objective

Gastric-type mucinous carcinoma (GAS), as a rare subtype of mucinous adenocarcinoma, accounts for approximately 1%–3% of cervical adenocarcinoma. It was considered as a new type of cervical mucinous adenocarcinoma by the World Health Organization (WHO) in 2014. GAS represents more aggressive disease than does usual type endocervical adenocarcinoma (UEA).

Case report

A case of cervical adenocarcinoma with an abnormal increase of CA199 in a 50-year-old Chinese woman was reported. Our patient presented with abnormal vaginal discharge and combined with elevated Ca199 at the value of 2,729 U/mL. Imaging examinations showed no abnormalities. Diagnostic conical resection suggested cervical adenocarcinoma in situ. Post-operative pathology confirmed mucinous cervical adenocarcinoma (considering gastric type), infiltrating cervical interstitial >2/3, involving the deep myometrium, accompanied by vascular carcinoma infiltration and lymph node metastasis.

The patients received an extensive hysterectomy and post-operative adjuvant chemoradiotherapy. The chemotherapy regimen was paclitaxel, combined with platinum. After 20 months of follow-up, the patient showed no signs of recurrence.

Conclusion

Preoperative diagnosis of cervical adenocarcinoma is insidious and can be easily misdiagnosed. For patients with high preoperative Ca199, the possibility of GAS should be kept open.
Contributions of Plant Litter Decomposition to Soil Nutrients in Ecological Tea Gardens
Authors: Liu Shaqian , Yang Rui , Peng Xudong , Hou Chunlan , Ma Juebing , Guo Jiarui
Published: July 2022

Plant litter decomposition and its effect on soil nutrients are important parts of the ecosystem material cycle, and understanding these processes is key for species selection and allocation to promote the effective use of litter in ecological tea gardens. In this study, the in situ litter decomposition method was used to examine the decomposition characteristics of leaf litter of Cinnamomum glanduliferum, Betula luminifera, Cunninghamia lanceolata, Pinus massoniana, and Camellia sinensis prunings in the Jiu’an ecological tea garden in Guizhou and their effects on soil nutrients. The results showed that the litter decomposition rate of broad-leaved tree species was higher than that of coniferous tree species, with a half-life of 1.11–1.75a and a turnover period of 4.79–7.57a. There are two release modes of nutrient release from litter: direct release and leaching–enrichment–release. Different litters make different contributions to soil nutrients; Betula luminifera and Cinnamomum glanduliferum litter increased the contents of soil organic carbon, soil total nitrogen, and soil hydrolyzed nitrogen. Betula luminifera litter increased the content of soil total phosphorus, soil available phosphorus, and soil available potassium, and Pinus massoniana litter increased the content of soil total potassium and soil available potassium; therefore, it is concluded that the decomposition of Betula luminifera litter had a positive effect on soil nutrient content. Thus, Betula luminifera is a good choice for inclusion in ecological tea gardens to increase their nutrient return capacity, maintain fertility, and generally promote the ecological development of tea gardens.
Landscape of Global Gene Expression Reveals Distinctive Tissue Characteristics in Bactrian Camels (Camelus bactrianus)
Authors: Luan Yuanyuan , Fang Yan , Jiang Lin , Ma Yuehui , Wu Shangjie , Zhou Junwen , Pu Yabin , Zhao Qianjun , He Xiaohong
Published: July 2022

Bactrian camels (Camelus bactrianus) are highly adapted to the desert and semi-desert environments of Asia and developed unique physiological adaptations to cold, heat, drought, and nutrient-poor conditions. These animals are an ideal model for studying desert adaptation. However, the transcriptome of different Bactrian camel tissues has not been profiled. This study performed a comprehensive transcriptome analysis of nine fetal and adult tissues. A total of 20,417 coding genes were identified, and 2.4 billion reads were generated. Gene expression and functional analyses revealed that approximately 50% of the identified genes were ubiquitously expressed, and one-third were tissue-elevated genes, which were enriched in pathways related to the biological functions of the corresponding tissue. Weighted gene co-expressed network analysis (WGCNA) identified four modules—fat metabolism, water balance, immunity, and digestion—and several hub genes, including APOA1, TMEM174, CXCL12, and MYL9. The analysis of differentially expressed genes (DEGs) between fetal and adult tissues revealed that downregulated genes were enriched in tissue development, whereas upregulated genes were enriched in biological function in adult camels. DEGs in the hump were enriched in immune-related pathways, suggesting that this tissue is involved in immunity. This study is the first to generate a transcriptome atlas of major tissues in Bactrian camels and explores the genes potentially involved in the adaptation to desert environments.
Land Use, Temperature, and Nitrogen Affect Nitrous Oxide Emissions in Amazonian Soils
Authors: Lage Filho Nauara M. , Cardoso Abmael da S. , Azevedo Jorge C. de , Faturi Cristian , da Silva Thiago C. , Domingues Felipe N. , Ruggieri Ana C. , Reis Ricardo A. , do Rêgo Aníbal C.
Published: July 2022

Nitrous oxide (N2O) is one of the main gases emitted from soils, and the changes in land use in the Amazon may alter gas emission patterns. The objective of this study was to evaluate the effects of land use, temperature, and nitrogen on N2O emissions in soils in the Amazon. For this, three treatments randomized, with five repetitions, were incubated to quantify N2O emissions: (i) three different land uses (wet rainforest, pasture, and agriculture); (ii) different temperatures (25, 30, 35, and 40 °C); and (iii) different nitrogen additions to the soil (0, 90, 180, and 270 kg of N ha−1). Our results show that land use alters the flux of N2O, with the highest emissions observed in agricultural soils compared to that in forest and pasture areas. The change in soil temperature to 30 °C increased N2O emissions with land use, at which the emission of N2O was higher in the pasture and agriculture soils. Our results showed that the emission of N2O in the soil of the Amazon rainforest was low regardless of the temperature and nitrogen treatment. Therefore, the change in land use alters the resilience of the ecosystem, providing emissions of N2O.
Study on the Forming Mechanism of the High-Density Spot of Locust Coupled with Habitat Dynamic Changes and Meteorological Conditions Based on Time-Series Remote Sensing Images
Authors: Guo Jing , Zhao Longlong , Huang Wenjiang , Dong Yingying , Geng Yun
Published: July 2022

The outbreak of the Asian migratory locust (Locusta migratoria migratoria) (AML) can deal a great blow to agriculture and grassland farming. The emergence of high-density locusts facilitates the outbreak of locusts. Understanding the forming mechanism of the high-density spot of locust (HDSL) is very important for locust monitoring and control. To achieve this goal, this paper took Nong’an County, which used to form an HDSL in 2017, as the study area. Firstly, based on the habitat classification system, support vector machine (SVM), random forest (RF), and maximum likelihood (ML) methods were employed to explore the best classification method for locust habitats. Then, the optimal method was applied to monitor habitat dynamic changes from 2014 to 2017 in the HDSL in Nong’an. Finally, the HDSL forming mechanism was clarified coupled with habitat dynamic changes and meteorological data. The results showed that the SVM method was the optimal method, with an accuracy of 95.28%, which is higher than the RF and ML methods by 0.25% and 8.52%, respectively. The annual increased barren land and sufficient reeds provided adequate suitable habitats for the breeding of AML. From 2014 to 2016, the temperatures during the overwintering and hatching periods were higher than the 2010–2018 average, and the precipitation during the spawning period was lower than the 2010–2018 average. The precipitation during the growing period in 2017 was 30.8 mm less than the average from 2010 to 2018. All these characteristics were conducive to the reproduction of locusts. We concluded that the suitable habitat and meteorological conditions increased the locust quantity yearly, resulting in the formation of HDSL. These results are instrumental for monitoring potential high-risk outbreak areas, which is important to improve locust control and ensure food security.
Deep-Sea Sediments from the Southern Gulf of Mexico Harbor a Wide Diversity of PKS I Genes
Authors: Fernández-López Maikel , Sánchez-Reyes Ayixon , Barcelos Clara , Sidón-Ceseña Karla , Leite Ricardo B. , Lago-Lestón Asunción
Published: July 2022

The excessive use of antibiotics has triggered the appearance of new resistant strains, which is why great interest has been taken in the search for new bioactive compounds capable of overcoming this emergency in recent years. Massive sequencing tools have enabled the detection of new microorganisms that cannot be cultured in a laboratory, thus opening the door to the search for new biosynthetic genes. The great variety in oceanic environments in terms of pressure, salinity, temperature, and nutrients enables marine microorganisms to develop unique biochemical and physiological properties for their survival, enhancing the production of secondary metabolites that can vary from those produced by terrestrial microorganisms. We performed a search for type I PKS genes in metagenomes obtained from the marine sediments of the deep waters of the Gulf of Mexico using Hidden Markov Models. More than 2000 candidate genes were detected in the metagenomes that code for type I PKS domains, while biosynthetic pathways that may code for other secondary metabolites were also detected. Our research demonstrates the great potential use of the marine sediments of the Gulf of Mexico for identifying genes that code for new secondary metabolites.