bluuebunny/crossref_metadata_2025 huggingface dataset:
Unveiling the structural transformation and activity origin of heteroatom-doped carbons for hydrogen evolution
Authors: Lu Shanshan , Cheng Chuanqi , Shi Yanmei , Wu Yongmeng , Zhang Zhipu , Zhang Bin
Published: May 2023

Heteroatom-doped carbon materials have been widely used in many electrocatalytic reduction reactions. Their structure-activity relationships are mainly explored based on the assumption that the doped carbon materials remain stable during electrocatalysis. However, the structural evolution of heteroatom-doped carbon materials is often ignored, and their active origins are still unclear. Herein, taking N-doped graphite flake (N-GP) as the research model, we present the hydrogenation of both N and C atoms and the consequent reconstruction of the carbon skeleton during the hydrogen evolution reaction (HER), accompanied by a remarkable promotion of the HER activity. The N dopants are gradually hydrogenated and almost completely dissolved in the form of ammonia. Theoretical simulations demonstrate that the hydrogenation of the N species leads to the reconstruction of the carbon skeleton from hexagonal to 5,7-topological rings (G5-7) with thermoneutral hydrogen adsorption and easy water dissociation. P-, S-, and Se-doped graphites also show similar removal of doped heteroatoms and the formation of G5-7 rings. Our work unveils the activity origin of heteroatom-doped carbon toward the HER and opens a door to rethinking the structure-performance relationships of carbon-based materials for other electrocatalytic reduction reactions.
Exceptional stratospheric contribution to human fingerprints on atmospheric temperature
Authors: Santer Benjamin D. , Po-Chedley Stephen , Zhao Lilong , Zou Cheng-Zhi , Fu Qiang , Solomon Susan , Thompson David W. J. , Mears Carl , Taylor Karl E.
Published: May 2023

In 1967, scientists used a simple climate model to predict that human-caused increases in atmospheric CO
2
should warm Earth’s troposphere and cool the stratosphere. This important signature of anthropogenic climate change has been documented in weather balloon and satellite temperature measurements extending from near-surface to the lower stratosphere. Stratospheric cooling has also been confirmed in the mid to upper stratosphere, a layer extending from roughly 25 to 50 km above the Earth’s surface (S
25 − 50
). To date, however, S
25 − 50
temperatures have not been used in pattern-based attribution studies of anthropogenic climate change. Here, we perform such a “fingerprint” study with satellite-derived patterns of temperature change that extend from the lower troposphere to the upper stratosphere. Including S
25 − 50
information increases signal-to-noise ratios by a factor of five, markedly enhancing fingerprint detectability. Key features of this global-scale human fingerprint include stratospheric cooling and tropospheric warming at all latitudes, with stratospheric cooling amplifying with height. In contrast, the dominant modes of internal variability in S
25 − 50
have smaller-scale temperature changes and lack uniform sign. These pronounced spatial differences between S
25 − 50
signal and noise patterns are accompanied by large cooling of S
25 − 50
(1 to 2

°

C over 1986 to 2022) and low S
25 − 50
noise levels. Our results explain why extending “vertical fingerprinting” to the mid to upper stratosphere yields incontrovertible evidence of human effects on the thermal structure of Earth’s atmosphere.
Antithetic effects of agonists and antagonists on the structural fluctuations of TRPV1 channel
Authors: Sumino Ayumi , Zhao Yimeng , Mukai Daichi , Sumikama Takashi , Puppulin Leonardo , Hattori Motoyuki , Shibata Mikihiro
Published: May 2023

Transient receptor potential vanilloid member 1 (TRPV1) is a heat and capsaicin receptor that allows cations to permeate and cause pain. As the molecular basis for temperature sensing, the heat capacity (Δ
C
p
) model [D. E. Clapham, C. Miller,
Proc. Natl. Acad. Sci. U.S.A.
108
, 19492–19497 (2011).] has been proposed and experimentally supported. Theoretically, heat capacity is proportional to a variance in enthalpy, presumably related to structural fluctuation; however, the fluctuation of TRPV1 has not been directly visualized. In this study, we directly visualized single-molecule structural fluctuations of the TRPV1 channels in a lipid bilayer with the ligands resiniferatoxin (agonist, 1,000 times hotter than capsaicin) and capsazepine (antagonist) by high-speed atomic force microscopy. We observed the structural fluctuations of TRPV1 in an apo state and found that RTX binding enhances structural fluctuations, while CPZ binding suppresses fluctuations. These ligand-dependent differences in structural fluctuation would play a key role in the gating of TRPV1.
A tessellated lymphoid network provides whole-body T cell surveillance in zebrafish
Authors: Robertson Tanner F. , Hou Yiran , Schrope Jonathan , Shen Simone , Rindy Julie , Sauer John-Demian , Dinh Huy Q. , Huttenlocher Anna
Published: May 2023

Homeostatic trafficking to lymph nodes allows T cells to efficiently survey the host for cognate antigen. Nonmammalian jawed vertebrates lack lymph nodes but maintain diverse T cell pools. Here, we exploit in vivo imaging of transparent zebrafish to investigate how T cells organize and survey for antigen in an animal devoid of lymph nodes. We find that naïve-like T cells in zebrafish organize into a previously undescribed whole-body lymphoid network that supports streaming migration and coordinated trafficking through the host. This network has the cellular hallmarks of a mammalian lymph node, including naïve T cells and CCR7-ligand expressing nonhematopoietic cells, and facilitates rapid collective migration. During infection, T cells transition to a random walk that supports antigen-presenting cell interactions and subsequent activation. Our results reveal that T cells can toggle between collective migration and individual random walks to prioritize either large-scale trafficking or antigen search in situ. This lymphoid network thus facilitates whole-body T cell trafficking and antigen surveillance in the absence of a lymph node system.
A large conserved family of small-molecule carboxyl methyltransferases identified from microorganisms
Authors: Lin Zhi , Hu Zhiwei , Zhou Linjun , Liu Benben , Huang Xiaowei , Deng Zixin , Qu Xudong
Published: May 2023

Small-molecule carboxyl methyltransferases (CbMTs) constitute a small proportion of the reported methyltransferases, but they have received extensive attention due to their important physiological functions. Most of the small-molecule CbMTs isolated to date originate from plants and are members of the SABATH family. In this study, we identified a type of CbMT (OPCMT) from a group of
Mycobacteria
, which has a distinct catalytic mechanism from the SABATH methyltransferases. The enzyme contains a large hydrophobic substrate-binding pocket (~400 Å
3
) and utilizes two conserved residues, Thr20 and Try194, to retain the substrate in a favorable orientation for catalytic transmethylation. The OPCMT_like MTs have a broad substrate scope and can accept diverse carboxylic acids enabling efficient production of methyl esters. They are widely (more than 10,000) distributed in microorganisms, including several well-known pathogens, whereas no related genes are found in humans. In vivo experiments implied that the OPCMT_like MTs was indispensable for
M. neoaurum
, suggesting that these proteins have important physiological functions.
Distinct domains in Ndc1 mediate its interaction with the Nup84 complex and the nuclear membrane
Authors: Amm Ingo , Weberruss Marion , Hellwig Andrea , Schwarz Johannes , Tatarek-Nossol Marianna , Lüchtenborg Christian , Kallas Martina , Brügger Britta , Hurt Ed , Antonin Wolfram
Published: May 2023

Nuclear pore complexes (NPCs) are embedded in the nuclear envelope and built from ∼30 different nucleoporins (Nups) in multiple copies, few are integral membrane proteins. One of these transmembrane nucleoporins, Ndc1, is thought to function in NPC assembly at the fused inner and outer nuclear membranes. Here, we show a direct interaction of Ndc1’s transmembrane domain with Nup120 and Nup133, members of the pore membrane coating Y-complex. We identify an amphipathic helix in Ndc1’s C-terminal domain binding highly curved liposomes. Upon overexpression, this amphipathic motif is toxic and dramatically alters the intracellular membrane organization in yeast. Ndc1’s amphipathic motif functionally interacts with related motifs in the C-terminus of the nucleoporins Nup53 and Nup59, important for pore membrane binding and interconnecting NPC modules. The essential function of Ndc1 can be suppressed by deleting the amphipathic helix from Nup53. Our data indicate that nuclear membrane and presumably NPC biogenesis depends on a balanced ratio between amphipathic motifs in diverse nucleoporins.
A core–shell magnet Mn70Bi30 grown at seeds in magnetic fields and its impacts on its spin-dynamics, Curie point and other tailored properties
Authors: Sharma Sanjeev Kumar , Pradhan Debabrata , Ram Shanker
Published: May 2023

The MnBi alloys is a model series of rare-Earth free magnets for surge of technologies of small parts of automobiles, power generators, medical tools, memory systems, and many others. The magnetics stem primarily at unpaired Mn-3d5 spins (a 4.23 μ
B moment) align parallel via an orbital moment 0.27 μ
B of Bi-5d106s2p3 in a crystal lattice. Thus, using a surplus Mn (over Bi) in a Mn70Bi30 type alloy designs a spin-rich system of duly tailored properties useful for magnetics and other devices. In this view, we report here a strategy of a refined alloy powder Mn70Bi30 can grow into small crystals of hexagonal (h) plates at seeds as annealed in magnetic fields (in H2 gas). So, small h-plates (30 to 50 nm widths) are grown up at (002) facets, wherein the edges are turned down in a spiral (≤2.1 nm thicknesses) in a core–shell structure. The results are described with x-ray diffraction, lattice images and magnetic properties of a powder Mn70Bi30 (milled in glycine) is annealed at 573 K for different time periods, so to the Mn/Bi order at the permeable facets (seeds). Duly annealed samples exhibit an enhanced magnetization, M
s → 70.8 emu g−1, with duly promoted coercivity H
c → 10.810 kOe (15.910 kOe at 350 K), energy–product 14.8 MGOe, and the crystal-field-anisotropy, K
1 → 7.6 × 107 erg cm−3, reported at room temperature. Otherwise, M
s should decline at any surplus 3d5-Mn spins order antiparallel at the antisites. Enhanced Curie point 658.1 K (628 K at Mn50Bi50 alloy) anticipates that a surplus Mn does favor the Mn–Bi exchange interactions. Proposed spin models well describe the spin-dynamics and lattice relaxations (on anneals) over the lattice volume (with twins) and spin clusters.
The early heading growth stage of rice plants in using the SRI method by giving liquid organic fertilizer of coconut husk and banana stems
Authors: Rozen N , Kasim M , Kusumawati A , Ikram H , Sholihat I
Published: May 2023

Coconut husk and banana stem waste can be used as a source of organic material for plants which is utilized in the form of liquid organic fertilizer. This study aims to determine the interaction between types of liquid organic fertilizer and the concentration of liquid organic fertilizer. This research was carried out in Sungai Bangek, Balai Gadang Village, Koto Tangah District, Padang City from June until September 2022. The design used was a split plot in a randomized block design. The main plot is liquid organic fertilizer with 2 levels of coconut husk and banana stem while the subplot is liquid organic fertilizer concentration consisting of 7 levels (0, 50, 100, 150, 200, 250, 300 ml/liter). The data were analyzed through variance analysis using the F test and if significant, it was continued with Duncan New Multiple Range Test (DNMRT) with a significance level of 5%. The data is processed with Stat8 software. The observed variables consisted of plant height, the number of tillers, leaf area index, relative growth rate, and net assimilation rate. The results obtained on the growth variable have not shown significant results in every observed observation. There was a very strong correlation between the number of tillers and the leaf area index which had a unidirectional relationship. There was a fairly strong correlation between the leaf area index and net assimilation rate which has an opposite relationship.
Preliminary results of the scale insects (Hemiptera: Sternorryncha) exploration from West Halmahera, in the Eastern Weber line of Wallacea
Authors: Hidayat P , Subagyo V N O , Maharani Y , Puspasari L T , Encilia  , Suwito A , Fara S B
Published: May 2023

Exploration of scale insects was done in West Halmahera, North Maluku, from May to June 2022 using purposive sampling as many 7,261 individual scale insects, which consist of six families, were collected. Basic information was recorded, such as pictures of insects, host plants, and GPS coordinates. Identification to species level is still ongoing by morphological characters using slide mounting specimens and the mtCO-I DNA sequencing. It is expected to have several new reports on insect species, host plants, and possibly new species. The information will enrich the record of fauna diversity in Indonesia and be useful for agriculture, especially in pest management.
Testing Theory-Based Messages to Encourage Women at Average Risk for Breast Cancer to Consider Biennial Mammography Screening
Authors: Guan Yue , Haardörfer Regine , McBride Colleen M , Escoffery Cam , Lipscomb Joseph
Published: May 2023

Background

The US Preventive Services Task Force does not recommend routine annual mammography screening for women aged 40–49 at average risk. Little research has been done to develop theory-based communication interventions to facilitate informed decision-making about reducing potentially low-value mammography screening.

Purpose

Evaluate the effects of theory-based persuasive messages on women’s willingness to consider delaying screening mammography until age 50 or have mammograms biennially.

Methods

We conducted a randomized controlled communication experiment online with a population-based sample of U.S. women aged 40–49 (N = 383) who screened to be at average risk for breast cancer. Women were randomly assigned to the following messaging summaries: annual mammography risks in 40s (Arm 1, n = 124), mammography risks plus family history-based genetic risk (Arm 2, n = 120), and mammography risks, genetic risk, and behavioral alternatives (Arm 3, n = 139). Willingness to delay screening or reduce screening frequency was assessed post-experiment by a set of 5-point Likert scale items.

Results

Women in Arm 3 reported significantly greater willingness to delay screening mammography until age 50 (mean = 0.23, SD = 1.26) compared with those in Arm 1 (mean = −0.17, SD = 1.20; p = .04). There were no significant arm differences in willingness to reduce screening frequency. Exposure to the communication messages significantly shifted women’s breast cancer-related risk perceptions without increasing unwarranted cancer worry across all three arms.

Conclusions

Providing women with screening information and options may help initiate challenging discussions with providers about potentially low-value screening.
ATTIC is an integrated approach for predicting A-to-I RNA editing sites in three species
Authors: Chen Ruyi , Li Fuyi , Guo Xudong , Bi Yue , Li Chen , Pan Shirui , Coin Lachlan J M , Song Jiangning
Published: May 2023

A-to-I editing is the most prevalent RNA editing event, which refers to the change of adenosine (A) bases to inosine (I) bases in double-stranded RNAs. Several studies have revealed that A-to-I editing can regulate cellular processes and is associated with various human diseases. Therefore, accurate identification of A-to-I editing sites is crucial for understanding RNA-level (i.e. transcriptional) modifications and their potential roles in molecular functions. To date, various computational approaches for A-to-I editing site identification have been developed; however, their performance is still unsatisfactory and needs further improvement. In this study, we developed a novel stacked-ensemble learning model, ATTIC (A-To-I ediTing predICtor), to accurately identify A-to-I editing sites across three species, including Homo sapiens, Mus musculus and Drosophila melanogaster. We first comprehensively evaluated 37 RNA sequence-derived features combined with 14 popular machine learning algorithms. Then, we selected the optimal base models to build a series of stacked ensemble models. The final ATTIC framework was developed based on the optimal models improved by the feature selection strategy for specific species. Extensive cross-validation and independent tests illustrate that ATTIC outperforms state-of-the-art tools for predicting A-to-I editing sites. We also developed a web server for ATTIC, which is publicly available at http://web.unimelb-bioinfortools.cloud.edu.au/ATTIC/. We anticipate that ATTIC can be utilized as a useful tool to accelerate the identification of A-to-I RNA editing events and help characterize their roles in post-transcriptional regulation.
Identification of representative species-specific genes for abundance measurements
Authors: Zachariasen Trine , Petersen Anders Østergaard , Brejnrod Asker , Vestergaard Gisle Alberg , Eklund Aron , Nielsen Henrik Bjørn
Published: May 2023

Motivation

Metagenomic binning facilitates the reconstruction of genomes and identification of Metagenomic Species Pan-genomes or Metagenomic Assembled Genomes. We propose a method for identifying a set of de novo representative genes, termed signature genes, which can be used to measure the relative abundance and used as markers of each metagenomic species with high accuracy.

Results

An initial set of the 100 genes that correlate with the median gene abundance profile of the entity is selected. A variant of the coupon collector’s problem was utilized to evaluate the probability of identifying a certain number of unique genes in a sample. This allows us to reject the abundance measurements of strains exhibiting a significantly skewed gene representation. A rank-based negative binomial model is employed to assess the performance of different gene sets across a large set of samples, facilitating identification of an optimal signature gene set for the entity. When benchmarked the method on a synthetic gene catalog, our optimized signature gene sets estimate relative abundance significantly closer to the true relative abundance compared to the starting gene sets extracted from the metagenomic species. The method was able to replicate results from a study with real data and identify around three times as many metagenomic entities.

Availability and implementation

The code used for the analysis is available on GitHub: https://github.com/trinezac/SG_optimization.

Supplementary information

Supplementary data are available at Bioinformatics Advances online.
STEMSIM: a simulator of within-strain short-term evolutionary mutations for longitudinal metagenomic data
Authors: Zhou Boyan , Li Huilin
Published: May 2023

Motivation

As the resolution of metagenomic analysis increases, the evolution of microbial genomes in longitudinal metagenomic data has become a research focus. Some software has been developed for the simulation of complex microbial communities at the strain level. However, the tool for simulating within-strain evolutionary signals in longitudinal samples is still lacking.

Results

In this study, we introduce STEMSIM, a user-friendly command-line simulator of short-term evolutionary mutations for longitudinal metagenomic data. The input is simulated longitudinal raw sequencing reads of microbial communities or single species. The output is the modified reads with within-strain evolutionary mutations and the relevant information of these mutations. STEMSIM will be of great use for the evaluation of analytic tools that detect short-term evolutionary mutations in metagenomic data.

Availability and implementation

STEMSIM and its tutorial are freely available online at https://github.com/BoyanZhou/STEMSim.
GTExVisualizer: a web platform for supporting ageing studies
Authors: Guzzi Pietro Hiram , Lomoio Ugo , Veltri Pierangelo
Published: May 2023

Motivation

Studying ageing effects on molecules is an important new topic for life science. To perform such studies, the need for data, models, algorithms, and tools arises to elucidate molecular mechanisms. GTEx (standing for Genotype-Tissue Expression) portal is a web-based data source allowing to retrieve patients’ transcriptomics data annotated with tissues, gender, and age information. It represents the more complete data sources for ageing effects studies. Nevertheless, it lacks functionalities to query data at the sex/age level, as well as tools for protein interaction studies, thereby limiting ageing studies. As a result, users need to download query results to proceed to further analysis, such as retrieving the expression of a given gene on different age (or sex) classes in many tissues.

Results

We present the GTExVisualizer, a platform to query and analyse GTEx data. This tool contains a web interface able to: (i) graphically represent and study query results; (ii) analyse genes using sex/age expression patterns, also integrated with network-based modules; and (iii) report results as plot-based representation as well as (gene) networks. Finally, it allows the user to obtain basic statistics which evidence differences in gene expression among sex/age groups.

Conclusion

The GTExVisualizer novelty consists in providing a tool for studying ageing/sex-related effects on molecular processes.

Availability and implementation

GTExVisualizer is available at: http://gtexvisualizer.herokuapp.com. The source code and data are available at: https://github.com/UgoLomoio/gtex_visualizer.
Aerobic exercises regulate the epididymal anion homeostasis of high-fat diet-induced obese rats through TRPA1-mediated Cl− and HCO3− secretion
Authors: Gao Dong-Dong , Ding Nan , Deng Wei-Ji , Li Pei-Lun , Chen Yi-Lin , Guo Lian-Meng , Liang Wen-Hao , Zhong Jia-Hui , Liao Jing-Wen , Huang Jun-Hao , Hu Min
Published: May 2023

Aerobic exercises could improve the sperm motility of obese individuals. However, the underlying mechanism has not been fully elucidated, especially the possible involvement of the epididymis in which sperm acquire their fertilizing capacity. This study aims to investigate the benefit effect of aerobic exercises on the epididymal luminal milieu of obese rats. Sprague–Dawley male rats were fed on a normal or high-fat diet (HFD) for 10 weeks and then subjected to aerobic exercises for 12 weeks. We verified that TRPA1 was located in the epididymal epithelium. Notably, aerobic exercises reversed the downregulated TRPA1 in the epididymis of HFD-induced obese rats, thus improving sperm fertilizing capacity and Cl− concentration in epididymal milieu. Ussing chamber experiments showed that cinnamaldehyd (CIN), agonist of TRPA1, stimulated an increase of the short-circuit current (ISC) in rat cauda epididymal epithelium, which was subsequently abolished by removing the ambient Cl− and HCO3−. In vivo data revealed that aerobic exercises increased the CIN-stimulated Cl− secretion rate of epididymal epithelium in obese rats. Pharmacological experiments revealed that blocking cystic fibrosis transmembrane regulator (CFTR) and Ca2+-activated Cl− channel (CaCC) suppressed the CIN-stimulated anion secretion. Moreover, CIN application in rat cauda epididymal epithelial cells elevated intracellular Ca2+ level, and thus activate CACC. Interfering with the PGHS2-PGE2-EP2/EP4-cAMP pathway suppressed CFTR-mediated anion secretion. This study demonstrates that TRPA1 activation can stimulate anion secretion via CFTR and CaCC, which potentially forming an appropriate microenvironment essential for sperm maturation, and aerobic exercises can reverse the downregulation of TRPA1 in the epididymal epithelium of obese rats.
OC-006 INTRAOPERATIVE FASCIA TRACTION FOR THE TREATMENT OF LARGE ABDOMINAL WALL/INCISIONAL HERNIAS (W3, EHS)
Authors: Niebuhr H , Dag H , Reinpold W
Published: May 2023

Aim

To evaluate the effectiveness, clinical practicability, and complication rate of the fascial traction procedure for the treatment of large ventral hernias.

Method

This observational study evaluated 90 patients from 1 specialised center with an intraoperatively measured fascial distance of more than 10 cm who were treated by Intraoperative Fascia Traction – IFT- (abdominal wall stretching) using the fasciotens® hernia traction procedure.

Results

Fascial gap measured pre-operatively ranged from 10 to 44 cm, with most patients (94%) having a fascial above 15 cm (W3 according to the European Hernia Society classification). The mean fascial distance was reduced from 16.1 ± 0.8 to 5.8 ± 0.7 cm (stretch gain 10.2 ± 0.7 cm, p < 0.0001, Wilcoxon matched-pairs signed-ranks test). A reduction in fascial distance of at minimum 50% was achieved in three quarters of the patients, and in half of the treated patients the reduction in fascial distance amounted to even more than 70% The closure rate achieved by fascial traction after a mean surgical duration of 207.3 ± 11.0 minutes was 90% (81/90).

Postoperative complications occurred in 7 patients (8%). Re-operation was required in 4 patients (4.5%).

Conclusion

This described intraoperative fascia traction (IFT) method is a new, promising procedure for abdominal wall closure in large ventral hernias. The presented results demonstrate a high effectiveness, a good clinical practicability and a low complication rate of the

Intraoperative fascia traction (IFT) method.
OC-012 NATIONWIDE ANALYSIS OF SHORT-TERM OUTCOMES AFTER LAPAROSCOPIC IPOM VS. ROBOT-ASSISTED RETROMUSCULAR REPAIR OF SMALL TO MEDIUM VENTRAL HERNIA
Authors: Jensen K K , Helgstrand F , Henriksen N A
Published: May 2023

Aim

With the introduction of a robot-assisted approach, retromuscular mesh placement is technically more feasible compared to laparoscopic IPOM, with potential gains for the patient, including avoidance of painful mesh fixation and intraperitoneal mesh placement. We aimed to examine the short-term outcomes after laparoscopic intraperitoneal onlay mesh (IPOM) compared to robot-assisted retromuscular repair of small to medium sized ventral hernia.

Material & Methods

This was a nationwide cohort study of all patients undergoing either laparoscopic IPOM or robot-assisted retromuscular repair of a ventral hernia with a horizontal fascial defect <7 cm in the period 2017 to 2022. Outcomes included postoperative hospital length of stay, 90-day readmission, and 90-day operative reintervention, and multivariable logistic regression analysis was performed to adjust for the relevant confounder.

Results

A total of 2,453 patients were included for analysis, of which 389 patients underwent robot-assisted retromuscular hernia repair and 2,064 laparoscopic IPOM repair.

The rate of IPOM repaired patients hospitalized more than 2 days was 4 times higher than after robotic retromuscular repair (18.2% vs. 4.5%, P < 0.001). The incidence of readmission within 90 days postoperatively was significantly higher after laparoscopic IPOM repair (10.9% vs. 6.7%, P = 0.016). There was no difference in the incidence of patients undergoing operative intervention within the first 90 days postoperatively (laparoscopic IPOM 1.8% vs. robot-assisted retromuscular 1.3%, P = 0.579).

Conclusions

Robot-assisted retromuscular ventral hernia repair was associated with a significantly reduced incidence of prolonged length of postoperative hospital stay and risk of 90-day compared to laparoscopic IPOM.
OC-013 MEDIUM-LONG TERM OUTCOMES OF RETROMUSCULAR PARASTOMAL HERNIA REPAIR WITH PAULI MODIFIED SUGAR BARKER PROCEDURE
Authors: Martínez Caballero J , Román García De León L , Medina Pedrique M , Avilés Oliveros A , Morejón Ruiz S , Pérez-Flecha González M , Robin Valle Del Lersundi A , Rodríguez Muñoz J , López Monclús J , Blazquez Hernando L A , García Ureña M Á
Published: May 2023

Objective

We aim to evaluate the outcomes and stoma complication in parastomal hernia repair (PHR) with Pauli procedure.

Material and Methods

18 patients were recruited between 2015–2022 in 2 hospitals. Data were obtained from prospective clinical database. Surgical technique included: posterior component separation (TAR), bowel lateralization according to Pauli modified Sugarbaker and, if necessary, stoma relocation. Quantitative variables are shown as median [interquartile rank] and qualitative as percentage.

Results

22.2% patients had recurrent PH with 2 [3] previous repair attempts. 22.2% underwent bilateral TAR and 11.1% required inlay mesh. Panniculectomy was performed in 17,8% of patients and stoma relocation in 44,4%; 17% required intestinal resection and 11,1% bowel suturing.

Complications < 30days: 17% had surgical site infection (SSIs), 2 deep SSIs and one required surgical management. 22.2% had clinical seroma, one required percutaneous drainage and other surgical intervention. Postoperative complications were adynamic ileus (22,2%), bowel obstruction (5%) which was managed conservatively and anastomotic leakage (5%). No mortality was observed, and hospital-stay length was 8 [7.25] days.

Complications > 30days: 5.5% chronic seroma (grade III), 5.5% PTE and 22.2% bowel obstruction of which one required adhesiolysis and another bowel resection.

27.8% had any stoma-related complication: partial ischemia (5.5%), retraction (11.1%), prolapse (5.5%) and obstruction (5.5%) managed conservatively. One patient required reoperation due to stoma perforation. We found 25% of clinical or radiological recurrence after 24 [20.1] months follow-up.

Conclusion

This technique provides acceptable recurrence rates of the PH, but is still high, with a non-negligible associated comorbidity.
OC-041 TOTALLY EXTRA-PERITONEAL REPAIR VERSUS TRANS-ABDOMINAL PRE-PERITONEAL REPAIR FOR THE LAPAROSCOPIC SURGICAL MANAGEMENT OF SPORTSMAN'S HERNIA: A SYSTEMATIC REVIEW AND META-ANALYSIS
Authors: Kler A , Sekhon N , Antoniou G , Satyadas T
Published: May 2023

Aim

Open and laparoscopic modalities are employed for treatment of sportsman's hernia, with totally extra-peritoneal (TEP) and trans-abdominal pre-peritoneal (TAPP) laparoscopic approaches both currently being utilised. At present, neither subtype has demonstrated superiority for sportsman's hernia repair. The aim of this review was to evaluate current evidence to ascertain whether there was a difference in laparoscopic techniques following sportsman's hernia repair.

Material & Methods

A systematic literature search was performed according to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement standards. Databases searched included PubMed, Scopus and Web of Science to identify all randomised controlled trials (RCTs) and observational studies. Risk of bias was assessed using the Cochrane risk of bias tool and Newcastle-Ottawa scale for RCTs and observational studies, respectively. The assessed outcomes included median time to return to sporting activity, complications and the degree of postoperative pain reduction within three months. Random effects model was used to calculate pooled proportion data where feasible. Subgroup analyses were also performed.

Results

28 studies were identified including 2 RCTs and 26 observational studies. No significant differences were observed between techniques in the primary or secondary outcomes. Significant heterogeneity was observed in all outcomes. This was signficant for return to sporting activity; thus meta-analysis was not feasible in this domain. Median time to return to sporting activity was 28 days for both techniques.

Conclusions

There is no difference in the primary and secondary outcomes in either technique. An RCT comparing TEP and TAPP repair is needed to provide definitive data.
OC-048 LONG TERM OUTCOMES AND QUALITY OF LIFE AFTER OPEN ABDOMEN TREATMENT
Authors: Theodorou A , Von Websky M
Published: May 2023

Aim

Open abdomen treatment (OAT) can manage severe abdominal conditions but is associated with high morbidity and mortality. Main risks are entero-atmospheric fistula (EAF), failure of primary fascial closure, and incisional hernias. In this study, we assessed long-term functional outcomes after OAT to understand which factors impacted most on quality of life (QoL).

Methods

After a retrospective analysis of 165 consecutive OAT patients treated in our center over a 10 years period, before the implantation of a structured mesh mediated fascial traction concept, a prospective follow-up was initiated. All survivors were invited for a follow-up. 40 datasets including clinical and social follow-up with SF-36 scores were analysed.

Results

Primary closure (PC) was achieved in 23% of patients. 3/4 of the others underwent a secondary-stage abdominal wall reconstruction (SSR), but 2/3 of them developed recurrent hernias. 55% percent of the patients with PC developed an incisional hernia, while 20% of all patients developed significant scarring. Scar pain was described by 15% of the patients as “moderate” and by 10% as “severe”. While hernia presence and scarring showed no impact, male sex and EAF formation significantly reduced QoL.

Discussion

Despite advantages, OAT is associated with high mortality and morbidity, especially in early era before the implementation of a structured concept. Follow-up revealed that hernia is high and that 25% of patients qualifying for a secondary reconstruction did not want surgery or were unfit. Sex and EAF formation impacted significantly on QoL, which was lower than in the general population.
OC-050 FULL ENDOSCOPIC RETROMUSCULAR ACCESS (ETEP) ASSOCIATED WITH TRANSVERSUS ABDOMINIS RELEASE (TAR) IN INCISIONAL HERNIA AFTER LIVER TRANSPLANTATION. CASE-CONTROL STUDY
Authors: Bellido-Luque J , Licardie E , Tejada A , Bellido-Luque A , Gomez Menchero J , Suarez Grau J M , Sanchez-Matamoros I , Nogales Muñoz A
Published: May 2023

eTEP is a new technique that minimizes the possibility of intraperitoneal lesions or adhesions formation.

Methods

From January 2018 to January 2023, patients with symptomatic right subcostal incisional hernia after liver transplantation were underwent to eTEP - TAR. The results were compared to those operated on using IPOM + from 2016 to 2018 in a prospectively maintained database. Variables: type of scar -Defect length/width-mesh area-surgical time- complications-conversion rate-Length of stay- Pain at 1°-7°-30–90 days- recurrence rate.

Results

21 patients were included in eTEP and 14 in IPOM groups. No differences were seen in preoperative measurements/type of scar.

defect length 6,3+/- 1 cm width 4,2+/- 0,3 cm in eTEP and 8,7+/-1,5 and 5,1+/-0,7 cm respectively in IPOM. Surgical time was longer in eTEP with significant differences (115+/- 16 vs 65+/- 7,1 minutes). Seromav11% in eTEP and 13% in IPOM. Length of stay 1,2 +/- 0,7 and 2,1+/-0,6 days in eTEP and IPOM groups respectively with significant improvement in favor of eTEP. Operative time was higher in eTEP (106,8 +/- 20,5 minutes) than IPOM+ group (61,4 +/- 18 minutes) with significant differences. Postoperative pain in eTEP showed significantly less pain on 1st, 7th and 30th than IPOM+. No differences when 90th day pain was compared. follow-up 20,9 +/- 6,5 months in eTEP group and 38,3 +/- 4,8 months in IPOM+ group. No recurrences were identified.

Conclusions

eTEP-TAR for incisional hernia after liver transplantation is an optimal approach due the benefits of the extraperitoneal approach.. Postoperative pain and surgical stay are improved.
OC-062 15-YEAR FOLLOW-UP AFTER INCISIONAL HERNIA REPAIR: DYNAMICS OF RECURRENCE, RISK FACTORS, HEALTH CARE UTILIZATION AND PATIENT-REPORTED SATISFACTION
Authors: Cengiz Y , Karlsson S , Bjorklund I , Israelsson L
Published: May 2023

Aim

Long-time results after Incisional hernia (IH) repair including recurrence rates with possible risk factors, long-term patient reported outcome (PROM) and health care utilization related.

Methods

Medical records and additional clinical/radiological examinations of IH repairs performed 2002–2013 . Time to recurrence, risk factors, and hazard ratios were calculated. A questionnaire was used for PROM.

Results

299 IH repairs were performed. 260 (87%) with a sublay mesh, 15 (5%) with other mesh techniques and 24 (8%) with other techniques. The recurrence rates at 2, 5, 10, and 15 years were 7%, 9%, 11%, and 12%, respectively. Suture repair, obesity, small mesh size and wound infection were risk factors while a non-closed anterior aponeurosis was not. 9 out of 10 patients were satisfied, and 3 out of four were free of any discomfort. Risk factors identified for discomfort were women and patients younger than 65 years while mesh size was not. 7,1% hospital visits and 21,4% readmissions occurred and the reoperation rate was 7,2%.

Conclusions

The recurrence rate within 15 years was quite low and did not increase substantially with time. The larger the mesh, the lower the rate of recurrence. Closure of the anterior aponeurosis did not correlate to the rate of recurrence. Patient-satisfaction was high with low rates of long-term discomfort. Health care utilization mostly occurred within 2 months. Sublay mesh repairs were performed by applying a standardized regime. Based on current findings the regime now includes always aiming at placing a largest mesh possible.
OC-068 PORTUGUESE NATIONAL TRAINING IN HERNIA AND ABDOMINAL WALL SURGERY – THE RESIDENTS’ PERSPECTIVE
Authors: Pais D , Bolota J , Barreira A L , Rolo Santos C , Alves R , Botelho P
Published: May 2023

Abdominal Wall surgery is often considered the initial step in the training of General Surgery residents. However, that training is not standardized.

The resident committee of the Portuguese Society of Hernia and Abdominal Wall (SPHPA) conducted a survey to the general surgery community, addressing training in inguinal and incisional hernia surgery. Its aim was to analyze the reality of training in abdominal wall surgery in Portugal.

100 answers were validated - 43 from specialists and 57 from residents; 56 from district hospitals and 44 from central hospitals. 57 had a formal unit dedicated to the abdominal wall in their institution.

On the treatment of inguinal hernia, 99% responded that most corrections are open although 80% answered that laparoscopic corrections were performed in their institution. 65.7% answered that laparoscopic correction was reserved for specialists but 36% considered that it was appropriate to start perfoming it in the 3rd year of residency.

As for incisional hernia, 90% answered that most procedures were open corrections, although 88% stated that laparoscopic corrections were also performed. 52% answered that residents operate laparoscopically and 19% only started in the specialty; 34% consider it appropriate to start this training in the 3rd year of residency.

In conclusion, the abdominal wall is a focus of interest in General Surgery departments in Portugal, most of them having dedicated units. There is a disparity between the laparoscopic practice and the general opinion of when it should start, exposing the need for greater standardization of the surgical training.
OC-076 LAPAROSCOPIC TAPP REPAIR FOR A LUMBAR HERNIA. STEP-BY-STEP SYSTEMATIZATION
Authors: Santilli O , Santilli H , Nardelli N
Published: May 2023

Aim

Lumbar hernias are a rare clinical entity and surgical treatment is constantly evolving and changing. We propose a systematization step-by-step guiding a safe and effective repair with similar conditions to the inguinal region.

Material & Methods

The patient lies down in a dorsal decubitus position with a 45° elevation of the hernia side. The first port is in the umbilicus access, and the accessory ports are along the middle line. Start the dissection by marking the anatomical limits that involve the semilunar lines, epigastric vessels, spine, and imaginary line between the iliac crest and 11 th rib. Releasing the peritoneal flat until visualizing the psoas iliacus and quadratus muscles until the 11 th ribs, preserving the avascular plane avoids nerves and vascular damage. Hernia sac reduction. Placement of a polypropylene mesh (24×22 cm) covering all the dissected areas. Mesh fixation using absorbable sutures. Closure of the peritoneum.Vacuum maneuver and bulge compression.

Results

Systematic standardization was applied to all patients. The mean operative time was 90 min. Outpatient surgery was achieved with a fast return to usual activity. The procedures were performed without intraoperative complications, mortality, or conversion to open surgery.

Conclusions

The step-by-step guide for landmarks and tips provides a safe and effective repair of this rare entity. Finally, clinical studies are needed to measure the impact of this technique's implementation in lumbar hernia repair.
OC-077 ONE STOP MULTI-SPECIALTY CHRONIC POSTOPERATIVE INGUINAL PAIN CLINIC - IMPLEMENTATION OUTCOMES
Authors: Cocciolo F , Birch A , Al-Abbasi I , Pawlak M
Published: May 2023

Aim/Objective

The aim on this presentation is to assess patients’ feedback as well as the outcomes of individualised treatment plans for patients assessed in the multidisciplinary team (MDT) clinic. The primary endpoint was quality of life and pain improvement 3 months after receiving treatment.

Material and methods

All patients reviewed in MDT CPIP clinic in the Comprehensive Hernia Centre between July 2021 and July 2022 were included in the study. Patients feedback as well as MDT individualised treatment plans were assessed. The outcomes, Visual Analogue Scale (VAS) and modified Activity Assessment Scale (mAAS) 3 months post intervention have been audited against data collected prior to intervention.

Results

We have received 57 referrals during the investigated period. 40 patients underwent MDT assessment with 55% receiving treatment and entering follow-up stage. In that group 26% had surgery, 35% had invasive non-surgical treatment (local injections and radiofrequency ablation), 39% were qualified for pharmacological treatment, together with physiotherapy and psychological support. Follow up data was collected for 19/22 patients. All patients had reported moderate to significant pain (VAS 4–10) on initial assessment and a significant reduction in pain (> 50% improvement in VAS) after treatment. Mean baseline mAAS score was 20.3± 7.3 (range 8 to 40). Mean change was - 10.6±2.6 with all patients reporting significant improvement (> 50%). Moreover, on a scale from 0 to 5, the average satisfaction score was 4.5.

Conclusion

Developing a multidisciplinary clinic to assess patients presenting with CPIP improved patients’ experiences and outcomes. Enhancing patient-centred care.
OC-099 ROBOTICALLY-ASSISTED ENHANCED-VIEW TOTALLY EXTRAPERITONEAL (R-ETEP) TECHNIQUE FOR ENDOSCOPIC RETROMUSCULAR HERNIA REPAIR. A RETROSPECTIVE ANALYSIS OF OUR FIRST 99 CASES
Authors: Vogel R , Heinzelmann F , Buechler P , Mueck B
Published: May 2023

Introduction

The availability of robotics has marked the beginning of a new era in hernia surgery. Due to its tiltable instruments the robot grants a dexterity which is not comparable to any conventional minimally invasive approach. In 2019 we have established r-eTEP-repair in our hospital.

The objective of this analysis is to share our experience of the first ninety-nine cases.

Materials and Methods

A retrospective analysis was carried out of all patients who underwent a r-eTEP repair in our hospital from September 2019 to January 2023 using gathered data out of our hospital information system (ORBIS Dedalus).

Results

Within the study period, we operated on a total of 99 patients. In 27 patients an extraperitoneal transversus abdominis release (eTAR) was performed. Complete closure of the hernia defect was possible in all cases and an uncoated synthetic mesh was placed in the retromuscular plane. In 3 cases a partial open hybrid procedure with robotic transversus abdominis release was performed. The median defect size was 25cm2 (2,25–375 cm2). The median mesh size was 540 cm2 (260–1350 cm2). The postoperative complication rate was 6.06%. There was one intraoperative complication and no abdominal reoperations. Median length of hospital stay was 3 days (2–10).

Conclusion

eTEP is a safe and minimally invasive procedure for a retromuscular mesh placement. In combination with a transversus abdominis release, even complex findings can comfortably be treated endoscopically.
OC-105 PARASTOMAL HERNIA REPAIRS: A RETROSPECTIVE NATIONAL COHORT STUDY IN THE REPUBLIC OF IRELAND
Authors: Chan K Y , Raftery N , Abdelhafiz T , Rayis A , Johnston S
Published: May 2023

Aim

In the context of improving colorectal cancer outcomes, post-survivorship quality of life has become an important outcome measure. Parastomal hernias and their associated morbidity remain largely under-reported and under-appreciated. Despite their burden, conservative management is common. This study aims to provide a national overview on the current trends in parastomal hernia repairs (PHRs).

Material and Methods

All PHRs performed in the country between 1/2017 to 7/2022 were identified retrospectively from the National Quality Assurance and Improvement System (NQAIS) database. Anonymised patient characteristics and quality indices were extracted for statistical analysis.

Results

A total of 565 PHRs, 64.1% elective and the remainder emergent, were identified across 27 hospitals. The 8 national colorectal units performed 67.3% of all repairs. While 42.3% of PHRs were standalone procedures, reversal of Hartmann's procedure was the commonest simultaneous procedure in the remainder. The median age, ASA and Charlson Co-Morbidity Index were 64 years (24–97), 3(1–5) and 3(0–48) respectively. Mean length of stay (LOS) was 16.2 days (SD=29.8). 29.0% of patients had at least 1 emergency readmission in 12 months, with a corresponding 12% 30-day readmission rate. Linear regression analysis associated ASA (95% CI 0.58–16.1, p<0.035) and emergency admissions (95% CI 5.9–25.5, P<0.002) with a significantly longer LOS, with the latter also associated with more frequent emergency re-admissions (95% CI 0.18–0.82, p<0.002).

Conclusion

Patients undergoing emergency PHR are subjected to longer stays and more frequent readmissions. A dedicated parastomal hernia registry will facilitate timely elective intervention, negating the undue morbidity and costs associated with emergency
OC-120 ROBOTIC TRANSABDOMINAL RETROMUSCULAR RECTUS DIASTASIS (R-TARRD) REPAIR - A NEW APPROACH
Authors: Tartaglia E , Cuccurullo D , Sagnelli C , Guerriero L
Published: May 2023

Aim

To present our innovative robotic approach for the treatment of rectus diastasis with concurrent primary or incisional ventral hernias.

Material & Methods

We performed 45 r-TARRD repairs for symptomatic rectus diastasis with concomitant associated ventral/incisional umbilical and/or epigastric hernias. Data on patient demographics, type of hernia, operative time, complications, recurrence rate and hospital stay were retrospectively analyzed. Follow-up was scheduled at 1 month, 6 months and 1 year after surgery.

Results

45 patients (13 M, 32 F) underwent r-TARRD repair. Mean age was 54,8 years (range 31–68) and mean BMI was 26,74 Kg/m2 (range 21,1–31). Mean ASA was 2,2 (range 1–3). In all patients we used a polypropylene mesh 25×15 cm, properly shaped. Mean operative time was 192 min (range115–260). Mean hospital stay 4,2 days (range 2–7). No conversion to laparoscopy or open surgery and no major complications occurred. At 1 month follow up one mesh infection (2,22%) was observed and it was treated conservatively. Four recurrences (8,88%) were reported at 1 year follow up.

Conclusions

Robot-assisted TARRD repair is conceived as a novel alternative minimally invasive procedure for RD with concurrent midline defects ensuring a primary fascial defect closure and mesh implantation in a sublay position with a wide overlap. It is important to better evaluate the suture that should be used to perform the repair and multicenter studies with standardization of patient's demographics, RD characteristics and long-term follow-up outcomes are mandatory to assess the effectiveness and durability of r-TARDD repair.