bluuebunny/crossref_metadata_2025 huggingface dataset:
Harnessing the cytotoxic granule exocytosis to augment the efficacy of T-cell-engaging bispecific antibody therapy.
Authors: Casey Mika , Lee Carol , Hoyte Sharon M. , Johnston Rebecca L. , Kwok Wing Yu , Law Soi Cheng , Gandhi Maher K , Harrison Simon J , Nakamura Kyohei
Published: January 2024

T-cell-engaging bispecific antibody (T-BsAb, also known as BiTE) therapy has emerged as a powerful therapeutic modality against multiple myeloma. Given that T-BsAb therapy redirects endogenous T cells to eliminate tumor cells, reinvigorating dysfunctional T cells may be a potential approach to improve the efficacy of T-BsAb. While various immunostimulatory cytokines can potentiate effector T-cell functions, the optimal cytokine treatment for T-BsAb therapy is yet to be established, partly due to a concern of cytokine release syndrome driven by aberrant interferon (IFN)-γ production. Here, we functionally screen immunostimulatory cytokines to determine an ideal combination partner for T-BsAb therapy. This approach reveals IL-21 as a potential immunostimulatory cytokine with the ability to augment T-BsAb-mediated release of granzyme B and perforin, without increasing IFN-γ release. Transcriptome profiling and functional characterization strongly support that IL-21 selectively targets the cytotoxic granule exocytosis pathway, but not pro-inflammatory responses. Notably, IL-21 modulates multiple steps of cytotoxic effector functions including upregulation of co-activating CD226 receptor, increasing cytotoxic granules, and promoting cytotoxic granule delivery at the immunological synapse. Indeed, T-BsAb-mediated myeloma-killing is cytotoxic granule-dependent, and IL-21 priming significantly augments cytotoxic activities. Furthermore, in vivo IL-21 treatment induces cytotoxic effector reprogramming in bone marrow T cells, showing synergistic anti-myeloma effects in combination with T-BsAb therapy. Together, harnessing the cytotoxic granule exocytosis pathway by IL-21 may be a potential approach to achieve better responses by T-BsAb therapy.
The clonal hydra: neoantigen-specific T-cell response in germ cell tumors
Authors: Mehra Shefali , Taylor Justin
Published: January 2024

Not available.
Determinants of Firm Value and Its Implications on Stock Return of Banking Companies in Indonesia
Authors: Wahyudi Slamet
Published: January 2024

This study aims to examine the effect of good corporate governance, macroeconomics, profitability and firm size on firm value which has implications for stock returns. Quantitative methods with panel data regression. The sample is 15 banking companies for the 2015-2021 period. The results show that 1) good corporate governance has no direct effect on stock returns but has a significant negative effect indirectly through firm value, 2) macroeconomics directly has a significant positive effect on stock returns but indirectly through firm value has no significant effect, 3 ) profitability either directly or indirectly through firm value has no significant effect on stock returns, 4) firm size either directly or indirectly through firm value has a significant positive effect on stock returns, 5) together good corporate governance, macro economy, profitability, and firm size have a significant positive effect on firm value, 6) together good corporate governance, macroeconomics, profitability, firm size and firm value have a significant positive effect on stock returns.
El papel transformador de la literatura infantil en la adquisición de la lectoescritura en niños y niñas
Authors: Bravo Ruiz Martha Magdalena , Galán-Giler María , Giler-Giler Rosa
Published: January 2024

El propósito del artículo es destacar la importancia de la literatura infantil con la finalidad de que el mediador involucre a los niños y niñas en la lectura y escritura a fin de despertar la creatividad y el desarrollo de la imaginación. Para ello, se plantean tres aspectos fundamentales: Recursos didácticos que promuevan la iniciación a la lectura y escritura; papel del padre y madre como promotor de la literatura infantil; y estrategias que promuevan rutinas y favorezcan el aprendizaje natural de la iniciación a la lectura y escritura. El análisis se fundamenta en el enfoque constructivista del aprendizaje del lenguaje. Se concluye con la mención de algunas consideraciones teórico-prácticas para mejorar el trabajo de la literatura infantil en el aula y en el hogar de los infantes. Para esta investigación se aplicó encuestas dirigidas a padres de familias y fichas de observación con destrezas de acuerdo al currículo de educación inicial aplicada a veinte niños y niñas de tres a cuatro años
El arte en el desarrollo de la imaginación y expresión en los niños de la Unidad Educativa Felipe Intriago Bravo, Manabí, Portoviejo
Authors: Anchundia-Rodríguez Stefany , Avilés-Tumbaco Ivanna , Molina-Ojeda Nancy
Published: January 2024

A través del presente estudio, se han podido identificar diversas características del arte que influyen en el desarrollo de la imaginación y expresión en los niños. Estas conclusiones derivan de una investigación realizada en la Unidad Educativa Felipe Intriago Bravo, ubicada en el cantón Portoviejo de la provincia de Manabí. Un hallazgo esencial obtenido de este trabajo es la destacada relevancia del arte en el fomento de la imaginación y la expresión en los niños. El arte proporciona un espacio de experimentación y de internalización a nivel cognitivo, permitiendo la conexión entre estructuras motoras, sensoriales y emocionales. Si bien las estrategias educativas tradicionales han mostrado buenos resultados en la enseñanza infantil, se hace patente la necesidad de diversificar los enfoques, particularmente en la enseñanza del arte. Se propone aprovechar el innato proceso de aprendizaje de los niños, incluyendo la estimulación creativa, la autonomía y la confianza, entre otros factores. En esta línea, se presenta una propuesta innovadora de apoyo que involucra el uso de un caballete para estimular y mejorar el desarrollo expresivo de los niños. Además, se llevó a cabo una encuesta a 5 docentes para explorar la utilización de técnicas innovadoras, como la implementación de un rincón de juego con un caballete. Como conclusión, este estudio enfatiza la importancia de continuar promoviendo prácticas que fomenten la imaginación y la expresión artística en la educación infantil. Es crucial considerar la diversidad de enfoques y perspectivas de los docentes, buscando enriquecer la educación artística en los niños y fortalecer su desarrollo integral.
Repair of Tracheo-Cutaneous Fistula Following Tracheostomy
Authors: Yoo Byung Joon , Ji Yong Bae , Song Chang Myeon , Tae Kyung
Published: January 2024

Tracheo-cutaneous fistula is a relatively uncommon complication that can occur after tracheotomy. The possibility of fistula increases in proportion to the duration of intubation and it occurs due to the transposition of stratified squamous epithelium in the passage between the tracheal mucosa and the skin. There are several surgical techniques to repair trachea-cutaneous fistula, such as primary suture, fistulectomy and primary suture, fistulectomy and secondary intention healing, and using local or regional flap. In addition to these techniques, we would like to introduce a technique used in our hospital.
PELATIHAN PUBLIC SPEAKING UNTUK MENUNJANG KEMAMPUAN PRESENTASI SISWA DI DESA SINDANGJAYA
Authors: Salma Zalfa Naufali Nadita , Asmara Dwi Aksa Yudha , Marginingsih Ria
Published: January 2024

Ability in public speaking is an important part of every activity, especially activities in the academic environment. In reality, there are still few school students who have public speaking skills. Departing from this, the author conducts Public Speaking Training which will be held at MTs Fatahillah Sindangjaya Village. The methods used in this training are material delivery, discussion and simulation. This study aims to present sources of related topics in an effort to support presentation skills for students located in Sindangjaya Village. Data in this study was collected using interviews, web scraping and documentation. The results of this study show that students can understand how to speak and present well and correctly. It is hoped that this activity can increase the literacy of students' abilities in public speaking which can be used as a key to the advancement of a civilization in a country.
Business Formation – The Key to a Successful Business
Authors: Omonije Ajibola
Published: January 2024

In the dynamic landscape of the business world, staying ahead of trends and anticipating new shifts is essential for entrepreneurs. Building a successful business requires more than just a great idea; it demands a strategic blend of skills, adaptive strategies, meticulous planning, human and capital resources, a robust structure, and an efficient management system capable of thriving in a competitive marketplace. According to the Forbes Finance Council, a mere 45.4% to 51% of businesses survive the critical first five years. Business formation spans various crucial aspects, including ideation, planning, legal compliance, sales, marketing, customer experience, human resources, finance, accounting, and overall administration. Entrepreneurs often encounter challenges, particularly in their early stages, which can significantly impact the business's trajectory. This article unveils the intricacies of key areas that entrepreneurs must carefully consider during the business inception phase. Focused discussions cover critical aspects such as aligning the business idea, navigating legal and financial considerations, implementing effective business management, adept human resource management, and delivering exceptional customer service. These pivotal areas form the foundation of a sustainable business model, providing invaluable insights for entrepreneurs aiming to not only survive but thrive in the competitive business landscape.
Altered knee kinematics after posterior cruciate ligament single-bundle reconstruction—a comprehensive prospective biomechanical in vivo analysis
Authors: Oehme Stephan , Moewis Philippe , Boeth Heide , Bartek Benjamin , von Tycowicz Christoph , Ehrig Rainald , Duda Georg N. , Jung Tobias
Published: January 2024

Purpose: Passive tibiofemoral anterior-posterior (AP) laxity has been extensively investigated after posterior cruciate ligament (PCL) single-bundle reconstruction. However, the PCL also plays an important role in providing rotational stability in the knee. Little is known in relation to the effects of PCL single-bundle reconstruction on passive tibiofemoral rotational laxity. Gait biomechanics after PCL reconstruction are even less understood. The aim of this study was a comprehensive prospective biomechanical in vivo analysis of the effect of PCL single-bundle reconstruction on passive tibiofemoral rotational laxity, passive anterior-posterior laxity, and gait pattern.

Methods: Eight patients undergoing PCL single-bundle reconstruction (seven male, one female, mean age 35.6 ± 6.6 years, BMI 28.0 ± 3.6 kg/m2) were analyzed preoperatively and 6 months postoperatively. Three of the eight patients received additional posterolateral corner (PLC) reconstruction. Conventional stress radiography was used to evaluate passive translational tibiofemoral laxity. A previously established rotometer device with a C-arm fluoroscope was used to assess passive tibiofemoral rotational laxity. Functional gait analysis was used to examine knee kinematics during level walking.

Results: The mean side-to-side difference (SSD) in passive posterior translation was significantly reduced postoperatively (12.1 ± 4.4 mm vs. 4.3 ± 1.8 mm; p < 0.01). A significant reduction in passive tibiofemoral rotational laxity at 90° knee flexion was observed postoperatively (27.8° ± 7.0° vs. 19.9° ± 7.5°; p = 0.02). The range of AP tibiofemoral motion during level walking was significantly reduced in the reconstructed knees when compared to the contralateral knees at 6-month follow-up (16.6 ± 2.4 mm vs. 13.5 ± 1.6 mm; p < 0.01).

Conclusion: PCL single-bundle reconstruction with optional PLC reconstruction reduces increased passive tibiofemoral translational and rotational laxity in PCL insufficient knees. However, increased passive tibiofemoral translational laxity could not be fully restored and patients showed altered knee kinematics with a significantly reduced range of tibiofemoral AP translation during level walking at 6-month follow-up. The findings of this study indicate a remaining lack of restoration of biomechanics after PCL single-bundle reconstruction in the active and passive state, which could be a possible cause for joint degeneration after PCL single-bundle reconstruction.
The chemoenzymatic synthesis of glycan-terminated oligo(Leu)x
Authors: Black Sarah P. , Liu Melinda , Castillo Cesar , Coradeli Wynne , Totsingan Filbert , Edson Cody B. , Khare Sagar D. , Gross Richard A.
Published: January 2024

Introduction: Glycopeptides contain carbohydrate moieties (glycans) covalently attached to the side chain and/or terminal peptide units. Since glycans are present on cell surfaces, these constructs can potentially address a wide array of therapeutic functions. To overcome the deficiencies associated with current synthetic routes to glycopeptides, such as costly processes and toxic reagents, this work aimed to develop versatile environmentally friendly protease-catalyzed peptide synthesis routes to peptides decorated with a glycan at their N-terminus.

Methods: “Grafters” were first synthesized that consist of a glycan conjugated directly, or through a spacer, to the amine group of L-Phe-ethyl ester (Phe-OEt). The role of Phe-OEt is to increase the conjugate’s recognition by the protease (papain) catalytic active site. A series of grafters were synthesized with variation of the glycan structure, linkage-chemistry, and presence of an oligo (ethylene glycol) “spacer” of varied length between the glycan and Phe-OEt moiety. High grafter efficiency will result by the successful acceptance of the grafter at the enzymes S1/S2 subsites, formation of an acyl enzyme complex and subsequent conversion to glycan-terminated oligo(Leu)x (x ≥ 1), as opposed to construction of non-glycan N-terminated oligo(Leu)x.

Results and discussion: While glycan-Phe-OEt grafters without a spacer between the glycan and Phe-OEt resulted in low grafter efficiency (8.3% ± 2.0%), insertion of a short oligo (ethylene glycol) spacer between the glycan and Phe-OEt moieties (glycan-PEGn-Phe-OEt, n ≥ 3) increased the grafter efficiency by 3-fold–24.5% ± 1.8%. In addition, computational modeling was performed using Rosetta software provided insights on a molecular level of how grafter efficiency is influenced by the PEG spacer length.
An experimental study on the influence of instructional mobile applications in enhancing listening comprehension of rural students in India
Authors: Raj Antony , Tomy Prajeesh
Published: January 2024

Introduction

Mobile learning (M-learning) is integral to language education, offering accessibility, portability, and diverse resources. It offers real-life language use with synchronous learning, flexibility, and an opportunity to engage with the global community. It allows learners to practice language skills from anywhere. This study aims to evaluate linguistic instructional mobile applications' effectiveness on listening comprehension among Indian rural students.

Methods

Pedagogic mobile applications were employed in an experimental study with 149 tertiary-level students from rural colleges in Tamil Nadu, India. Participants were divided into control (n = 75) and experimental (n = 74) groups. Pre and post-tests assessed listening comprehension levels, considering demographic variables. Different types of audio files were used in the intervention to improve the listening skills of the experimental group.

Results

Statistical analyses, including paired t-tests, independent t-tests, and ANOVA, revealed significant improvement in listening skills for the experimental group. Gender, locality, and parental income were considered as significant variables, showing that students from diverse backgrounds benefited from mobile applications.

Discussion

The results indicate that M-learning effectively aids rural students in overcoming language barriers, emphasizing the potential of mobile applications in language education.
Undergraduates’ perceptions of the value of service-learning
Authors: Drewery Merritt L. , Lollar Jonathan
Published: January 2024

Service-learning integrates community service with academic study. Students apply skills and knowledge in practical situations and reflect on their experiences. Our 3000-level service-learning college course, Leadership Development in Agricultural Sciences, involved student-led development and execution of a one-day event, “AgCamp,” held on a university campus for high school students. The objective of this study was to evaluate if an intervention (i.e., a service-learning project) changed undergraduate students’ perceptions of their ability to collaborate, ability to lead, and overall perceptions of the usefulness of service-learning. An electronic mixed methods questionnaire-based survey was developed, tested for validity and reliability, and distributed to college students enrolled in Leadership Development in Agricultural Sciences as a pre- and post-test at the beginning and end of two semesters. Data were analyzed in SPSS 26.0 using measures of central tendency and paired sample t-tests. Undergraduate students’ (n = 74) had positive perceptions of service-learning entering the semester, all measures of which improved as a result of their experiences in the course. In comparison with traditional courses, students felt service-learning courses have more societal benefits and should be increasingly offered. Participating in the service-learning project significantly increased students’ comfort working with people from different cultures or backgrounds and empowered them in different ways, including with decision-making. Ultimately, our data indicate that students enjoyed service-learning and received soft skill development through the course. We recommend service-learning be increasingly integrated into post-secondary curriculum.
A practical guide to the diagnosis and management of osteoporosis in childhood and adolescence
Authors: Ward Leanne M.
Published: January 2024

Osteoporosis in childhood distinguishes itself from adulthood in four important ways: 1) challenges in distinguishing otherwise healthy children who have experienced fractures due to non-accidental injury or misfortunate during sports and play from those with an underlying bone fragility condition; 2) a preponderance of monogenic “early onset” osteoporotic conditions that unveil themselves during the pediatric years; 3) the unique potential, in those with residual growth and transient bone health threats, to reclaim bone density, structure, and strength without bone-targeted therapy; and 4) the need to benchmark bone health metrics to constantly evolving “normal targets”, given the changes in bone size, shape, and metabolism that take place from birth through late adolescence. On this background, the pediatric osteoporosis field has evolved considerably over the last few decades, giving rise to a deeper understanding of the discrete genes implicated in childhood-onset osteoporosis, the natural history of bone fragility in the chronic illness setting and associated risk factors, effective diagnostic and monitoring pathways in different disease contexts, the importance of timely identification of candidates for osteoporosis treatment, and the benefits of early (during growth) rather than late (post-epiphyseal fusion) treatment. While there has been considerable progress, a number of unmet needs remain, the most urgent of which is to move beyond the monotherapeutic anti-resorptive landscape to the study and application of anabolic agents that are anticipated to not only improve bone mineral density but also increase long bone cross-sectional diameter (periosteal circumference). The purpose of this review is to provide a practical guide to the diagnosis and management of osteoporosis in children presenting to the clinic with fragility fractures, one that serves as a step-by-step “how to” reference for clinicians in their routine clinical journey. The article also provides a sightline to the future, emphasizing the clinical scenarios with the most urgent need for an expanded toolbox of effective osteoporosis agents in childhood.
Meta-reservoir computing for learning a time series predictive model of wind power
Authors: Zhang Li , Ai Han-Xiao , Li Ya-Xin , Xiao Li-Xin , Dong Cao
Published: January 2024

Wind energy has become an essential part of the energy power source of current power systems since it is eco-friendly and sustainable. To optimize the operations of wind farms with the constraint of satisfying the power demand, it is critical to provide accurate predictions of wind power generated in the future. Although deep learning models have greatly improved prediction accuracy, the overfitting issue limits the application of deep learning models trained under one condition to another. A huge number of data are required to train a new deep learning model for another environment, which is sometimes not practical in some urgent situations with only very little training data on wind power. In this paper, we propose a novel learning method, named meta-reservoir computing (MRC), to address the above issue, combining reservoir computing and meta-learning. The reservoir computing method improves the computational efficiency of training a deep neural network for time series data. On the other hand, meta-learning is used to improve the initial point and other hyperparameters of reservoir computing. The proposed MRC method is validated using an experimental dataset of wind power compared with the traditional training method. The results show that the MRC method can obtain an accurate predictive model of wind power with only a few shots of data.
Conservation status of Brazilian snakes inhabiting the Atlantic Forest of Northeastern Brazil
Authors: França Rafaela C. , Solé Mirco , França Frederico G. R.
Published: January 2024

Due to the high level of disturbance in natural ecosystems and the progressive loss of habitats resulting from anthropic occupation, biodiversity conservation represents one of the greatest challenges today. Red lists of threatened species are essential tools for identifying species at risk of extinction and guiding conservation efforts. In this study, we assessed the vulnerability to extinction of 55 snake species that occur in the Atlantic Forest of northeastern Brazil in Paraíba state. We developed vulnerability indices based on 12 factors known to influence the survival of snake populations. To analyze the threat profiles and relative risk levels within the snake community, we employed principal component analysis (PCA) and cluster analysis. Additionally, we compared our findings with existing red lists of threatened species. Our results reveal that only 18% of the snake fauna in this region is free of any threat. The aquatic species Helicops angulatus and Oxyrhopus trigeminus were the snakes that presented the lowest risk of extinction, while Caaeteboia gaeli and Crotalus durissus presented the highest risk of extinction. Two groups of species were considered non-threatened and five groups were considered threatened. Our study provides the first overview on the conservation status of snake species in the northern portion of the Atlantic Forest and contributes to a better evaluation of conservation planning for this group in the region.
Characterizing Foxp3+ and Foxp3- T cells in the homeostatic state and after allo-activation: resting CD4+Foxp3+ Tregs have molecular characteristics of activated T cells
Authors: Liu Zilei , Baines Katherine J. , Niessen Natalie M. , Heer Munish K. , Clark David , Bishop G. Alexander , Trevillian Paul R.
Published: January 2024

Due to the intracellular expression of Foxp3 it is impossible to purify viable Foxp3+ cells on the basis of Foxp3 staining. Consequently CD4+Foxp3+ regulatory T cells (Tregs) in mice have mostly been characterized using CD4+CD25+ T cells or GFP-Foxp3 reporter T cells. However, these two populations cannot faithfully represent Tregs as the expression of CD25 and Foxp3 does not completely overlap and GFP+Foxp3+ reporter T cells have been reported to be functionally altered. The aim of this study was to characterize normal Tregs without separating Foxp3+ and Foxp3- cells for the expression of the main functional molecules and proliferation behaviors by flow cytometry and to examine their gene expression characteristics through differential gene expression. Our data showed that the expressions of Foxp3, CD25, CTLA-4 (both intracellular and cell surface) and PD-1 was mostly confined to CD4+ T cells and the expression of Foxp3 did not completely overlap with the expression of CD25, CTLA-4 or PD-1. Despite higher levels of expression of the T cell inhibitory molecules CTLA-4 and PD-1, Tregs maintained higher levels of Ki-67 expression in the homeostatic state and had greater proliferation in vivo after allo-activation than Tconv. Differential gene expression analysis revealed that resting Tregs exhibited immune activation markers characteristic of activated Tconv. This is consistent with the flow data that the T cell activation markers CD25, CTLA-4, PD-1, and Ki-67 were much more strongly expressed by Tregs than Tconv in the homeostatic state.
Case report: Cytopenias in VEXAS syndrome - a WHO 2022 based approach in a single-center cohort
Authors: Diral Elisa , Campochiaro Corrado , Tomelleri Alessandro , Bergonzi Gregorio M. , Pizzano Umberto , Ponzoni Maurilio , Bongiovanni Lucia , Ronchi Paola , Tresoldi Cristina , Rigamonti Silvia , Scarfò Federico , Latino Gloria M. , Rinaldi Emma , Bernardi Massimo , Dagna Lorenzo , Ciceri Fabio
Published: January 2024

VEXAS syndrome is an acquired autoinflammatory disease characterized in most cases by cytopenias and macrocytic anemia. Dyshematopoiesis is a frequent finding in chronic inflammatory conditions and therefore, cytopenias are not easily classified in VEXAS patients. Here we report a series of 7 patients affected by VEXAS associated cytopenias, treated at our center. The use of NGS, together with morphological assays, integrated with the WHO 2022 criteria, allowed to identify three subsets of VEXAS associated cytopenias: ICUS (idiopathic cytopenia of uncertain significance), CCUS (clonal cytopenia of uncertain significance) at high risk of clonal evolution, and MDS. This approach could help to better understand the nature of VEXAS associated cytopenias and to guide the use of specific targeted treatments in order to achieve long lasting responses.
Nitrogen and phosphorus uptake kinetics in cultures of two novel picoplankton groups responsible for a recent bloom event in a subtropical estuary (Indian River Lagoon, Florida)
Authors: Papacek Joshua R. , Inglett Patrick W. , Phlips Edward J. , Lasi Margaret A.
Published: January 2024

Introduction

Successful management and mitigation of harmful algal blooms (HABs) requires an in-depth understanding of the physiology and nutrient utilization of the organisms responsible. We explored the preference of various nitrogen (N) and phosphorus (P) substrates by two novel groups of HAB-forming phytoplankton originating from the Indian River Lagoon (IRL), Florida: 1) a consortium of picocyanobacteria (Crocosphaera sp. and ‘Synechococcus’ sp.) and 2) ananochlorophyte (Picochlorum sp.).

Methods

Short-term kinetic uptake experiments tested algal use and affinity for inorganic and organic N substrates (ammonium (NH4+), nitrate (NO3-), urea, and an amino acid (AA) mixture) through 15N and 13C isotope tracing into biomass.

Results

Picocyanobacteria exhibited Michaelis-Menten type uptake for the AA mixture only, while nanochlorophytes reached saturation for NH4+, the AA mixture, and urea at or below 25 µM-N. Both picocyanobacteria and nanochlorophyte cultures had highest affinity (Vmax/Ks) for NH4+ followed by the AA mixture and urea. Neither culture showed significant uptake of isotopically-labeled nitrate. Disappearance of glucose-6-phosphate (G6P) added to culture medium suggesting use of organic P by both cultures was confirmed by detection of alkaline phosphatase activity and the tracing of 13C-G6P into biomass.

Discussion

Together, our results suggest that these HAB-forming phytoplankton groups are able to use a variety of N and P sources including organic forms, and prefer reduced forms of N. These traits are likely favorable under conditions found in the IRL during periods of significant competition for low concentrations of inorganic nutrients. Bloom-forming phytoplankton are therefore able to subsist on organic or recycled forms of N and P that typically dominate the IRL nutrient pools.
Machine learning–based feature prediction of convergence zones in ocean front environments
Authors: Xu Weishuai , Zhang Lei , Wang Hua
Published: January 2024

The convergence zone holds significant importance in deep-sea underwater acoustic propagation, playing a pivotal role in remote underwater acoustic detection and communication. Despite the adaptability and predictive power of machine learning, its practical application in predicting the convergence zone remains largely unexplored. This study aimed to address this gap by developing a high-resolution ocean front-based model for convergence zone prediction. Out of 24 machine learning algorithms tested through K-fold cross-validation, the multilayer perceptron–random forest hybrid demonstrated the highest accuracy, showing its superiority in predicting the convergence zone within a complex ocean front environment. The research findings emphasized the substantial impact of ocean fronts on the convergence zone’s location concerning the sound source. Specifically, they highlighted that in relatively cold (or warm) water, the intensity of the ocean front significantly influences the proximity (or distance) of the convergence zone to the sound source. Furthermore, among the input features, the turning depth emerged as a crucial determinant, contributing more than 25% to the model’s effectiveness in predicting the convergence zone’s distance. The model achieved an accuracy of 82.43% in predicting the convergence zone’s distance with an error of less than 1 km. Additionally, it attained a 77.1% accuracy in predicting the convergence zone’s width within a similar error range. Notably, this prediction model exhibits strong performance and generalizability, capable of discerning evolving trends in new datasets when cross-validated using in situ observation data and information from diverse sea areas.
Re-framing bio-plausible collision detection: identifying shared meta-properties through strategic prototyping
Authors: Wu Haotian , Yue Shigang , Hu Cheng
Published: January 2024

Insects exhibit remarkable abilities in navigating complex natural environments, whether it be evading predators, capturing prey, or seeking out con-specifics, all of which rely on their compact yet reliable neural systems. We explore the field of bio-inspired robotic vision systems, focusing on the locust inspired Lobula Giant Movement Detector (LGMD) models. The existing LGMD models are thoroughly evaluated, identifying their common meta-properties that are essential for their functionality. This article reveals a common framework, characterized by layered structures and computational strategies, which is crucial for enhancing the capability of bio-inspired models for diverse applications. The result of this analysis is the Strategic Prototype, which embodies the identified meta-properties. It represents a modular and more flexible method for developing more responsive and adaptable robotic visual systems. The perspective highlights the potential of the Strategic Prototype: LGMD-Universally Prototype (LGMD-UP), the key to re-framing LGMD models and advancing our understanding and implementation of bio-inspired visual systems in robotics. It might open up more flexible and adaptable avenues for research and practical applications.
The Mongolian gerbil as an advanced model to study cone system physiology
Authors: Günter Alexander , Belhadj Soumaya , Seeliger Mathias W. , Mühlfriedel Regine
Published: January 2024

In this work, we introduce a diurnal rodent, the Mongolian gerbil (Meriones unguiculatus) (MG) as an alternative to study retinal cone system physiology and pathophysiology in mice. The cone system is of particular importance, as it provides high-acuity and color vision and its impairment in retinal disorders is thus especially disabling. Despite their nocturnal lifestyle, mice are currently the most popular animals to study cone-related diseases due to the high availability of genetically modified models. However, the potential for successful translation of any cone-related results is limited due to the substantial differences in retinal organization between mice and humans. Alternatively, there are diurnal rodents such as the MG with a higher retinal proportion of cones and a macula-like specialized region for improved visual resolution, the visual streak. The focus of this work was the evaluation of the MG’s cone system functionality using full-field electroretinography (ERG), together with a morphological assessment of its retinal/visual streak organization via angiography, optical coherence tomography (OCT), and photoreceptor immunohistochemistry. We found that rod system responses in MGs were comparable or slightly inferior to mice, while in contrast, cone system responses were much larger, more sensitive, and also faster than those in the murine counterparts, and in addition, it was possible to record sizeable ON and OFF ERG components. Morphologically, MG cone photoreceptor opsins were evenly distributed throughout the retina, while mice show a dorsoventral M- and S-opsin gradient. Additionally, each cone expressed a single opsin, in contrast to the typical co-expression of opsins in mice. Particular attention was given to the visual streak region, featuring a higher density of cones, elongated cone and rod outer segments (OSs), and an increased thickness of the inner and outer retinal layers in comparison to peripheral regions. In summary, our data render the MG a supreme model to investigate cone system physiology, pathophysiology, and to validate potential therapeutic strategies in that context.
Central nervous system involvement in chronic inflammatory demyelinating polyradiculoneuropathy—MRS and DTI study
Authors: Dziadkowiak Edyta , Koszewicz Magdalena , Podgórski Przemysław , Wieczorek Małgorzata , Budrewicz Sławomir , Zimny Anna
Published: January 2024

Objective

The current research aimed to analyze the alterations within the motor cortex and pyramidal pathways and their association with the degree of damage within the peripheral nerve fibers in patients with chronic inflammatory demyelinating polyradiculoneuropathy (CIDP). To achieve that goal, we investigated the microstructural changes within the pyramidal white matter tracts using diffusion tensor imaging (DTI) parameters, evaluated metabolic alterations in both precentral gyri using magnetic resonance spectroscopy (MRS) ratios, and correlated them with the neurographic findings in patients with CIDP.

Methods

The spectroscopic ratios of NAA/Cr, Cho/Cr, and mI/Cr from both precentral gyri and the values of fractional anisotropy (FA), axial diffusivity (AD), and mean diffusivity (MD) from both of the corticospinal tracts were correlated with the results of neurological and neurographic findings. The comparison of DTI parameters between the patients and controls was performed using Student’s t-test or the Mann–Whitney U test. Due to the lack of normal distribution of most variables, Spearman’s Rho rank coefficient was used to test all correlations. All analyses were performed at a significant level of alpha = 0.05 using STATISTICA 13.3.

Results

Compared to the control group (CG), the patient group showed significantly lower ratios of NAA/Cr (1.66 ± 0.11 vs. 1.61 ± 0.15; p = 0.022), higher ratios of ml/Cr in the right precentral gyrus (0.57 ± 0.15 vs. 0.61 ± 0.08; p = 0.005), and higher levels of Cho/Cr within the left precentral gyrus (0.83 ± 0.09 vs. 0.88 ± 0.14, p = 0.012). The DTI parameters of MD from the right CST and AD from the right and left CSTs showed a strong positive correlation (0.52–0.53) with the sural sensory nerve action potential (SNAP) latency of the right sural nerve. There were no other significant correlations between other DTI and MRS parameters and neurographic results.

Significance

In our study, significant metabolic alterations were found in the precentral gyri in patients with CIDP without clinical symptoms of central nervous system involvement. The revealed changes reflected neuronal loss or dysfunction, myelin degradation, and increased gliosis. Our results suggest coexisting CNS damage in these patients and may provide a new insight into the still unknown pathomechanism of CIDP.
In-depth analysis of immune cell landscapes reveals differences between lung adenocarcinoma and lung squamous cell carcinoma
Authors: Wang Xinfeng , Zheng Keao , Hao Zhiying
Published: January 2024

Background

Lung cancer is the leading cause of cancer deaths globally, with lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC) being major subtypes. Immunotherapy has emerged as a promising approach for the treatment of lung cancer, but understanding the underlying mechanisms of immune dysregulation is crucial for the development of effective therapies. This study aimed to investigate the distinctive cellular features of LUAD and LUSC and identify potential biomarkers associated with the pathogenesis and clinical outcomes of each subtype.

Methods

We used digital cytometry techniques to analyze the RNA-Seq data of 1128 lung cancer patients from The Cancer Genome Atlas (TCGA) database. The abundance of cell subtypes and ecotypes in LUAD and LUSC patients was quantified. Univariate survival analysis was used to investigate their associations with patient overall survival (OS). Differential gene expression analysis and gene co-expression network construction were carried out to explore the gene expression patterns of LUSC patients with distinct survival outcomes. Scratch wound-healing assay, colony formation assay, and transwell assay were used to validate the candidate drugs for LUSC treatment.

Results

We found differential expression of cell subtypes between LUAD and LUSC, with certain cell subtypes being prognostic for survival in both subtypes. We also identified differential gene expression and gene co-expression modules associated with macrophages.3/PCs.2 ratio in LUSC patients with distinct survival outcomes. Furthermore, ecotype ratios were found to be prognostic in both subtypes and machine learning models showed that certain cell subtypes, such as epithelial.cells.1, epithelial.cells.5, and endothelial.cells.2 are important for predicting LUSC. Ginkgolide B and triamterene can inhibit the proliferation, invasion, and migration of LUSC cell lines.

Conclusion

We provide insight into the distinctive cellular features of LUAD and LUSC, and identify potential biomarkers associated with the pathogenesis and clinical outcomes of each subtype. Ginkgolide B and triamterene could be promising drugs for LUSC treatment.
Effect of dexmedetomidine on postoperative nausea and vomiting in female patients undergoing radical thoracoscopic lung cancer resection
Authors: Zhu Haipeng , Wang Shichao , Wang Ruohan , Li Bing , Zhang Jiaqiang , Zhang Wei
Published: January 2024

Introduction: Postoperative nausea and vomiting (PONV) is a prevalent postsurgical complication. The objective of our study was to compare the effect of different doses of dexmedetomidine on PONV in female patients undergoing radical thoracoscopic lung cancer resection.

Methods: A total of 164 female patients undergoing elective thoracoscopic radical lung cancer surgery were enrolled and assigned to one of four groups. Patients received 0.2 μg/kg/h, 0.4 μg/kg/h, 0.8 μg/kg/h dexmedetomidine and normal saline in the Dex1, Dex2, Dex3 and Control groups, respectively. The primary outcome was the incidence of PONV during 48 h postoperatively. The second outcomes included the incidence of PONV and postoperative vomiting (POV) at four time points postoperatively (T1: PACU retention period; T2: PACU discharge to postoperative 12 h; T3: postoperative 12 h-postoperative 24 h; T4: postoperative 24 h-postoperative 48 h), the area under the curve of PONV grade (PONVAUC), PONV grade, POV grade and other postoperative recovery indicators.

Results: The incidence of PONV differed among the four groups. The Dex2 group (29.27%) was lower than that in the Dex1 group (61.90%) and Control group (72.50%). The incidence of PONV at T2 in the Dex1 group (11.90%) and Dex2 group (9.76%) was lower than that in the Control group (42.50%). The incidence of PONV at T3 in the Dex2 group (29.27%) was lower than that in the Dex1 group (61.90%) and Control group (62.50%). The PONVAUC was lower in the Dex2 group than in the Control group. The incidence of POV at T3 in the Dex2 and Dex3 groups was lower than that in the Control group. The consumption of remifentanil, norepinephrine, PACU dwell time, VAS scores, postoperative PCA press frequency, and the time for the first postoperative oral intake were different among the four groups. The regression model shows that the Dex2 group is a protective factor for PONV.

Conclusion: Dexmedetomidine can reduce the incidence of PONV and accelerate postoperative recovery in female patients undergoing radical thoracoscopic lung cancer resection. Compared with the other two dosages, 0.4 μg/kg/h dexmedetomidine is preferable.

Clinical Trial Registration:chictr.org.cn, identifier ChiCTR2300071831
AI algorithm for personalized resource allocation and treatment of hemorrhage casualties
Authors: Jin Xin , Frock Andrew , Nagaraja Sridevi , Wallqvist Anders , Reifman Jaques
Published: January 2024

A deep neural network-based artificial intelligence (AI) model was assessed for its utility in predicting vital signs of hemorrhage patients and optimizing the management of fluid resuscitation in mass casualties. With the use of a cardio-respiratory computational model to generate synthetic data of hemorrhage casualties, an application was created where a limited data stream (the initial 10 min of vital-sign monitoring) could be used to predict the outcomes of different fluid resuscitation allocations 60 min into the future. The predicted outcomes were then used to select the optimal resuscitation allocation for various simulated mass-casualty scenarios. This allowed the assessment of the potential benefits of using an allocation method based on personalized predictions of future vital signs versus a static population-based method that only uses currently available vital-sign information. The theoretical benefits of this approach included up to 46% additional casualties restored to healthy vital signs and a 119% increase in fluid-utilization efficiency. Although the study is not immune from limitations associated with synthetic data under specific assumptions, the work demonstrated the potential for incorporating neural network-based AI technologies in hemorrhage detection and treatment. The simulated injury and treatment scenarios used delineated possible benefits and opportunities available for using AI in pre-hospital trauma care. The greatest benefit of this technology lies in its ability to provide personalized interventions that optimize clinical outcomes under resource-limited conditions, such as in civilian or military mass-casualty events, involving moderate and severe hemorrhage.
A comparative study of grain quality and physicochemical properties of premium japonica rice from three typical production regions
Authors: Dou Zhi , Yang Qian , Guo Halun , Zhou Yicheng , Xu Qiang , Gao Hui
Published: January 2024

Grain quality indicates rice commodity value. This research compared grain quality and physicochemical properties of premium japonica rice from three production regions, Yangtze River downstream of China (YRDCN), Northeast region of China (NECN) and Japan. Results showed that there were distinct quality and physicochemical characteristics variance among the three groups of japonica rice, while CVs of most quality parameters from low to high was Japan, YRDCN and NECN. YRDCN rice presented obvious lower apparent amylose content (AAC) and ratio of each chain-length sections of amylopectin, and showed higher protein contents especially glutelin and ratio in short and intermediate amylopectin molecules. Among three rice groups, YRDCN rice presented weaker appearance, whereas did not show inferior cooking and eating properties, which was primarily linked to lower AAC. Rice AAC and starch fine structure significantly correlated with pasting parameters, swelling power and solubility, while protein content had close relation with taste analyzer parameters. Results of this study indicated improvement direction for japonica rice of YRDCN, and also provided reference for consumers’ rice purchasing selection in accordance with individual taste preference.
Simon Fraser University Speech Error Database (SFUSED) Cantonese: Methods, design, and usage
Authors: Alderete John
Published: January 2024

The Simon Fraser University Speech Error Database (SFUSED) is a multi-purpose database of speech errors based in audio recordings. The motivation for SFUSED Cantonese, a component of this database, is to create a linguistically rich data set for exploring language production processes in Cantonese, an under-studied language. We describe in detail the methods used to collect, analyze, and explore the database, including details of team workflows, time budgets, data quality, and explicit linguistic and processing assumptions. In addition to showing how to use the database, this account supports future research with a template for investigating additional under-studied languages, and it gives fresh perspective on the benefits and drawbacks of collecting speech error data from spontaneous speech. All of the data and supporting materials are available as open access data sets.
Modeling of slip rate-dependent traversability for path planning of wheeled mobile robot in sandy terrain
Authors: Sakayori Go , Ishigami Genya
Published: January 2024

A planetary exploration rover has been employed for scientific endeavors or as a precursor for upcoming manned missions. Predicting rover traversability from its wheel slip ensures safe and efficient autonomous operations of rovers on deformable planetary surfaces; path planning algorithms that reduce slips by considering wheel-soil interaction or terrain data can minimize the risk of the rover becoming immobilized. Understanding wheel-soil interaction in transient states is vital for developing a more precise slip ratio prediction model, while path planning in the past assumes that slips generated at the path is a series of slip ratio in steady state. In this paper, we focus on the transient slip, or slip rate the time derivative of slip ratio, to explicitly address it into the cost function of path planning algorithm. We elaborated a regression model that takes slip rate and traction force as inputs and outputs slip ratio, which is employed in the cost function to minimize the rover slip in path planning phase. Experiments using a single wheel testbed revealed that even with the same wheel traction force, the slip ratio varies with different slip rates; we confirmed that the smaller the absolute value of the slip rate, the larger the slip ratio for the same traction force. The statistical analysis of the regression model confirms that the model can estimate the slip ratio within an accuracy of 85% in average. The path planning simulation with the regression model confirmed a reduction of 58% slip experienced by the rover when driving through rough terrain environments. The dynamics simulation results insisted that the proposed method can reduce the slip rate in rough terrain environments.