bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization of a Novel Family IV Esterase Containing a Predicted CzcO Domain and a Family V Esterase with Broad Substrate Specificity from an Oil-Polluted Mud Flat Metagenomic Library
Authors: Park Jong Eun , Jeong Geum Seok , Lee Hyun Woo , Kim Sung Kyum , Kim Jungho , Kim Hoon
Published: June 2021

Two novel esterase genes, est2L and est4L, were identified from a previously constructed metagenomic library derived from an oil-polluted mud flat sample. The encoded Est2L and Est4L were composed of 839 and 267 amino acids, respectively, without signal peptides. Est2L was a unique fusion type of protein composed of two domains: a domain of the CzcO superfamily, associated with a cationic diffusion promoter with CzcD, and a domain of the acetylesterase superfamily, belonging to family IV with conserved motifs, such as HGG, GXSAG, and GXPP. Est2L was the first fused esterase with a CzcO domain. Est4L belonged to family V with GXS, GXSMGG, and PTL motifs. Native Est2L and Est4L were found to be in dimeric and tetrameric forms, respectively. Est2L and Est4L showed the highest activities at 60 °C and 50 °C, respectively, and at a pH of 10.0. Est2L preferred short length substrates, especially p-nitrophenyl (pNP)-acetate, with moderate butyrylcholinesterase activity, whereas Est4L showed the highest activity with pNP-decanoate and had broad specificity. Significant effects were not observed in Est2L from Co2+ and Zn2+, although Est2L contains the domain CzcD. Est2L and Est4L showed high stabilities in 30% methanol and 1% Triton X-100. These enzymes could be used for a variety of applications, such as detergent and mining processing under alkaline conditions.
Digital Twin for Supply Chain Coordination in Modular Construction
Authors: Lee Dongmin , Lee SangHyun
Published: June 2021

Over the past decades, the construction industry has been attracted to modular construction because of its benefits for reduced project scheduling and costs. However, schedule deviation risks in the logistics process of modular construction can derail its benefits and thus interfere with its widespread application. To address this issue, we aim to develop a digital twin framework for real-time logistics simulation, which can predict potential logistics risks and accurate module arrival time. The digital twin, a virtual replica of the physical module, updates its virtual asset based on building information modeling (BIM) in near real-time using internet of thing (IoT) sensors. Then, the virtual asset is transferred and exploited for logistics simulation in a geographic information system (GIS)-based routing application. We tested this framework in a case project where modules are manufactured at a factory, delivered to the site via a truck, and assembled onsite. The results show that potential logistical risks and accurate module arrival time can be detected via the suggested digital twin framework. This paper’s primary contribution is the development of a framework that mediates IoT, BIM, and GIS for reliable simulation which predicts potential logistics risks and accurate module delivery time. Such reliable risk prediction enables effective supply chain coordination, which can improve project performance and the widespread application of modular construction.
Compliant Human–Robot Collaboration with Accurate Path-Tracking Ability for a Robot Manipulator
Authors: Reyes-Uquillas Daniel , Hsiao Tesheng
Published: June 2021

In this article, we aim to achieve manual guidance of a robot manipulator to perform tasks that require strict path following and would benefit from collaboration with a human to guide the motion. The robot can be used as a tool to increase the accuracy of a human operator while remaining compliant with the human instructions. We propose a dual-loop control structure where the outer admittance control loop allows the robot to be compliant along a path considering the projection of the external force to the tangential-normal-binormal (TNB) frame associated with the path. The inner motion control loop is designed based on a modified sliding mode control (SMC) law. We evaluate the system behavior to forces applied from different directions to the end-effector of a 6-DOF industrial robot in a linear motion test. Next, a second test using a 3D path as a tracking task is conducted, where we specify three interaction types: free motion (FM), force-applied motion (FAM), and combined motion with virtual forces (CVF). Results show that the difference of root mean square error (RMSE) among the cases is less than 0.1 mm, which proves the feasibility of applying this method for various path-tracking applications in compliant human–robot collaboration.
Review on the Service Safety Assessment of Main Cable of Long Span Multi-Tower Suspension Bridge
Authors: Wang Dagang , Ye Jihong , Wang Bo , Wahab Magd Abdel
Published: June 2021

The long-span multi-tower suspension bridge is widely used in the construction of river and sea crossing bridges. The load-bearing safety and anti-sliding safety of its main cable are directly related to the structural safety of a suspension bridge. Failure mechanisms of the main cable of a long-span multi-tower suspension bridge are discussed. Meanwhile, the tribo-corrosion-fatigue of main cable, contact, and slip behaviors of the saddle and service safety assessment of the main cable are reviewed. Finally, research trends in service safety assessment of main cable are proposed. It is of great significance to improve the service safety of the main cable and thereby to ensure the structural safety of long-span multi-tower suspension bridges.
Shugoshin: From the Perspective of Clinical Disorders
Authors: Kumar Ravinder , Agarwal Meenakshi
Published: June 2021

Proper and timely segregation of the cellular genome is a prime requirement of all cell division programs. Mis-segregation of chromosomes and the resulting aneuploidy lead to several clinical consequences. Over the years, shugoshin has emerged as a key protein factor involved in the segregation of genetic material in dividing cells. Deletion or an altered level of shugoshin is reported in several human malignancies; as a result, shugoshin is now emerging as an important tumor-associated gene and a possible target for cancer therapy. Apart from its role in cancer, recent studies have also shown the involvement of shugoshin in several other clinical disorders. In this review, we aim to highlight the clinical relevance of shugoshin.
Quantitative Assessment of Periodontal Bacteria Using a Cell-Based Immunoassay with Functionalized Quartz Crystal Microbalance
Authors: Rodphukdeekul Satit , Tabata Miyuki , Ratanaporncharoen Chindanai , Takeuchi Yasuo , Somboon Pakpum , Boonlue Watcharee , Miyahara Yuji , Sriyudthsak Mana
Published: June 2021

Periodontal disease is an inflammatory disorder that is triggered by bacterial plaque and causes the destruction of the tooth-supporting tissues leading to tooth loss. Several bacteria species, including Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, are considered to be associated with severe periodontal conditions. In this study, we demonstrated a quartz crystal microbalance (QCM) immunoassay for quantitative assessment of the periodontal bacteria, A. actinomycetemcomitans. An immunosensor was constructed using a self-assembled monolayer of 11-mercaptoundecanoic acid (11-MUA) on the gold surface of a QCM chip. The 11-MUA layer was evaluated using a cyclic voltammetry technique to determine its mass and packing density. Next, a monoclonal antibody was covalently linked to 11-MUA using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide to act as the biorecognition element. The specificity of the monoclonal antibody was confirmed by an enzyme-linked immunosorbent assay. A calibration curve, for the relationship between the frequency shifts and number of bacteria, was used to calculate the number of A. actinomycetemcomitans bacteria in a test sample. Based on a regression equation, the lower detection limit was 800 cells, with a dynamic range up to 2.32 × 106 cells. Thus, the QCM biosensor in this study provides a sensitive and label-free method for quantitative analysis of periodontal bacteria. The method can be used in various biosensing assays for practical application and routine detection of periodontitis pathogens.
Bifunctionalized Gold Nanoparticles for the Colorimetric Detection of the Drug γ-Hydroxybutyric Acid (GHB) in Beverages
Authors: Rodríguez-Nuévalos Silvia , Costero Ana M. , Gil Salvador , Parra Margarita , Gaviña Pablo
Published: June 2021

The increase in the number of drug-facilitated sexual assault (DFSA) cases in recent years has become a major concern. Consequently, there is a need to develop methods for the real-time detection of these substances. We report herein a colorimetric chemosensor for the real-time in situ detection of the “date rape” drug GHB. The sensor is based on gold nanoparticles functionalized with both a 2-aminonaphthoxazole and phenanthroline derivative. Its ability to act as “naked-eye” colorimetric sensor for the detection of the drug in soft drinks and alcoholic beverages was studied. The detection process is based on the double recognition of both the hydroxyl and the carboxylate groups present in GHB, which triggers the aggregation of the AuNPs, with the resulting change in the color of the solution.
A Simple and Rapid Spectrophotometric Method for Nitrite Detection in Small Sample Volumes
Authors: Thipwimonmas Yudtapum , Jaidam Janjira , Samoson Kritsada , Khunseeraksa Vacharachai , Phonchai Apichai , Thiangchanya Adul , Chang Kah Haw , Abdullah Ahmad Fahmi Lim , Limbut Warakorn
Published: June 2021

A simple, rapid, and environmentally-friendly spectrophotometric method for nitrite detection was developed. Detection was based on a redox reaction with iodide ions in an acidic condition. The reaction was evaluated by detecting the increase in absorbance of the colored product of iodine at 362 nm wavelength. To obtain a good spectrophotometric performance, the iodide ions concentration, hydrochloric acid concentration, and reaction time were optimized. In the optimal condition, the developed spectrophotometric method provided a linear range of 0.0625 to 4.00 mg L−1 (r = 0.9985), reaction time for 10 min, a limit of detection of 25 µg L−1, and a limit of quantitation of 85 µg L−1. This method showed good repeatability (RSD < 9.21%), high sample throughput (9 samples min−1), and good accuracy (recovery = 88 ± 2 to 99.5 ± 0.4%). The method has the potential to be used in crime scene investigations as a rapid screening test for gunshot residue detection via nitrite detection.
Novel Approach to Improving Knee Range of Motion in Arthrogryposis with a New Working Classification
Authors: Feldman David S. , Rand Troy J. , Huser Aaron J.
Published: June 2021

Arthrogryposis multiplex congenita (AMC) is a rare condition defined as contrac-tures in multiple joints. Surgical interventions for severe knee flexion contractures have included posterior release, distraction and extension with external fixation and distal femoral extension osteotomies. These operations have been able to achieve knee extension, but not increase the range of motion. The purpose of this study was to review our experience with peroneal nerve decompression, posterior knee release and proximal femoral shortening. We retrospectively reviewed the medical charts and radiographs of all patients with a diagnosis of arthrogryposis who underwent aforementioned procedure. There were 39 patients with 73 knees included in the analysis with a mean follow-up of 21 months. The mean preoperative arc of motion was 45° and last followup arc of motion was 79° (p < 0.0001). The mean last followup flexion contracture was 8° (p < 0.0001). Additional subanalyses were performed on those with followup greater than 24 months and those with flexion contractures >60°; there were no differences found in these groups. This study demonstrates that it is possible to achieve a functional range of motion of the knees in patients with AMC while improving ambulatory function.
Cross-Union Surgery for Congenital Pseudarthrosis of the Tibia
Authors: Shannon Claire E. , Huser Aaron J. , Paley Dror
Published: June 2021

Congenital Pseudoarthrosis of the Tibia (CPT) is a rare condition with a reputation for recurrent fractures and failure to achieve union. A large variety of surgical procedures have been attempted for the treatment of fractured cases of CPT with an average rate of union without refracture of only 50%. Intentional cross-union between the tibia and fibula has been reported to improve these results to 100% union with no refractures. This is a retrospective study of 39 cases of CPT in 36 patients treated by the Paley cross-union protocol with internal fixation, bone grafting, zoledronic acid infusion and bone morphogenic protein 2 (BMP2) insertion. All 39 cases of CPT united at the tibia and developed a cross-union to the fibula. Two patients had a persistent fibular pseudarthrosis, one that was later treated at the time of planned rod exchange and one that has remained asymptomatic. There were few postoperative complications. There were no refractures during the up to 7-year follow-up period. The most common problem was the Fassier-Duval (FD) rod pulling through the proximal or distal physis into the metaphysis (66.7%). This did not negatively affect the results and was remedied at the time of the planned rod exchange. The Paley Cross-Union Protocol is very technically demanding, but the results have radically changed the prognosis of this once sinister disease.
Maternal Sedentary Behavior and Physical Activity across Pregnancy and Early Childhood Motor Development
Authors: Jones Melissa A. , Whitaker Kara M. , Taverno Ross Sharon E. , Davis Kelliann , Libertus Klaus , Barone Gibbs Bethany
Published: June 2021

Early childhood motor development is an important indicator of short- and long-term health. In utero exposures impact offspring health across the lifespan; however, whether maternal activity during pregnancy may impact early childhood motor development remains unknown. This prospective cohort study measured the motor development skills of n = 70 children born to mothers from a previously conducted cohort study which objectively measured activity profile, (sedentary behavior (SED) and moderate-to-vigorous intensity physical activity (MVPA), across pregnancy. Mothers reported the motor development of their child using the Early Motor Questionnaire (EMQ). Linear regression models examined associations between maternal activity profile and EMQ scores. Maternal SED and MVPA were analyzed in two ways: trimester-specific and across pregnancy using trajectory groups. Children were 12–30 months of age, majority white (82%), and 52% male. Maternal SED during pregnancy was not associated with any EMQ domains (gross motor, fine motor, and perception action). Higher maternal MVPA, across pregnancy by trajectory group and in the first and second trimesters, was significantly associated with moderate-sized effects of more advanced fine motor and perception action scores. Higher MVPA in early pregnancy appears to be related to more advanced early childhood motor development. Therefore, maternal MVPA may be a modifiable behavior by which short- and long-term offspring health may be impacted.
Date Fruit and Seed in Nutricosmetics
Authors: Alharbi Khlood Lafi , Raman Jegadeesh , Shin Hyun-Jae
Published: June 2021

Many recent studies in the field of cosmetics have focused on organically sourced substances. Products made from organic materials are safe, high quality, cruelty-free, and more effective than those made from synthetic materials. Many organic compounds are known to be physiologically active in humans and have an extended storage capacity and long-lasting environmental effects. Agro-industrial waste has recently increased substantially, and the disposal of date palm waste, often performed in primitive ways such as burning, is harmful to the environment. Fruit processing industries generate over 10% of the total date seed waste daily, which could be converted into useful food products. Date fruit and seed are rich in sugar, vitamins, fiber, minerals, and phenolic compounds with antioxidant and anti-inflammatory properties that significantly promote human and animal health. This waste is rich in bioactive compounds and essential oils used in many kinds of food, medicine, and cosmetics. Most active cosmetic ingredients come from natural sources such as fruit, fish, and dairy, and recent research shows that date extract and seed oil help to reduce melanin, eczema, acne, and dry patches, while increasing skin moisture and elasticity. This review details the bioactive compounds and nutraceutical properties of date fruit and seed, and their use as cosmetic ingredients.
The Potential of 2-aza-8-Oxohypoxanthine as a Cosmetic Ingredient
Authors: Aoshima Hisae , Ito Masayuki , Ibuki Rinta , Kawagishi Hirokazu
Published: June 2021

In this study, we verified the effects of 2-aza-8-oxohypoxanthine (AOH) on human epidermal cell proliferation by performing DNA microarray analysis. Cell proliferation was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, which measures mitochondrial respiration in normal human epidermal keratinocyte (NHEK) cells. Gene expression levels were determined by DNA microarray analysis of 177 genes involved in skin aging and disease. AOH showed a significant increase in cell viability at concentrations between 7.8 and 31.3 μg/mL and a significant decrease at concentrations above 250 μg/mL. DNA microarray analysis showed that AOH significantly increased the gene expression of CLDN1, DSC1, DSG1, and CDH1 (E-cadherin), which are involved in intercellular adhesion and skin barrier functioning. AOH also up-regulated the expression of KLK5, KLK7, and SPIMK5, which are proteases involved in stratum corneum detachment. Furthermore, AOH significantly stimulated the expression of KRT1, KRT10, TGM1, and IVL, which are considered general differentiation indicators, and that of SPRR1B, a cornified envelope component protein. AOH exerted a cell activation effect on human epidermal cells. Since AOH did not cause cytotoxicity, it was considered that the compound had no adverse effects on the skin. In addition, it was found that AOH stimulated the expression levels of genes involved in skin barrier functioning by DNA microarray analysis. Therefore, AOH has the potential for practical use as a cosmetic ingredient. This is the first report of efficacy evaluation tests performed for AOH.
Entropy-Based Greedy Algorithm for Decision Trees Using Hypotheses
Authors: Azad Mohammad , Chikalov Igor , Hussain Shahid , Moshkov Mikhail
Published: June 2021

In this paper, we consider decision trees that use both conventional queries based on one attribute each and queries based on hypotheses of values of all attributes. Such decision trees are similar to those studied in exact learning, where membership and equivalence queries are allowed. We present greedy algorithm based on entropy for the construction of the above decision trees and discuss the results of computer experiments on various data sets and randomly generated Boolean functions.
Hybrid Nanofluids Flows Determined by a Permeable Power-Law Stretching/Shrinking Sheet Modulated by Orthogonal Surface Shear
Authors: Roşca Natalia C. , Pop Ioan
Published: June 2021

The present paper studies the flow and heat transfer of the hybrid nanofluids flows induced by a permeable power-law stretching/shrinking surface modulated orthogonal surface shear. The governing partial differential equations were converted into non-linear ordinary differential equations by using proper similarity transformations. These equations were then solved applying a numerical technique, namely bvp4c solver in MATLAB. Results of the flow field, temperature distribution, reduced skin friction coefficient and reduced Nusselt number were deduced. It was found that increasing mass flux parameter slows down the velocity and, hence, decreases the temperature. Furthermore, on enlarging the stretching parameter, the velocity and temperature increases and decreases, respectively. In addition, that the radiation parameter can effectively control the thermal boundary layer. Finally, the temperature decreases when the values of the temperature parameter increases. We apply similarity transformation in order to transform the governing model into a system of ODEs (ordinary differential equations). Numerical solutions for particular values of involved parameters are in very good agreement with previous calculations. The most important and interesting result of this paper is that for both the cases of shrinking and stretching sheet flows exhibit dual solutions in some intervals of the shrinking and stretching parameter. In spite of numerous published papers on the flow and heat transfer over a permeable stretching/shrinking surface in nanofluids and hybrid nanofluids, none of the researchers studied the present problem. Therefore, we believe that the results of the present paper are new, and have many industrial applications.
The AI-Atlas: Didactics for Teaching AI and Machine Learning On-Site, Online, and Hybrid
Authors: Stadelmann Thilo , Keuzenkamp Julian , Grabner Helmut , Würsch Christoph
Published: June 2021

We present the “AI-Atlas” didactic concept as a coherent set of best practices for teaching Artificial Intelligence (AI) and Machine Learning (ML) to a technical audience in tertiary education, and report on its implementation and evaluation within a design-based research framework and two actual courses: an introduction to AI within the final year of an undergraduate computer science program, as well as an introduction to ML within an interdisciplinary graduate program in engineering. The concept was developed in reaction to the recent AI surge and corresponding demand for foundational teaching on the subject to a broad and diverse audience, with on-site teaching of small classes in mind and designed to build on the specific strengths in motivational public speaking of the lecturers. The research question and focus of our evaluation is to what extent the concept serves this purpose, specifically taking into account the necessary but unforeseen transfer to ongoing hybrid and fully online teaching since March 2020 due to the COVID-19 pandemic. Our contribution is two-fold: besides (i) presenting a general didactic concept for tertiary engineering education in AI and ML, ready for adoption, we (ii) draw conclusions from the comparison of qualitative student evaluations (n = 24–30) and quantitative exam results (n = 62–113) of two full semesters under pandemic conditions with the result of previous years (participants from Zurich, Switzerland). This yields specific recommendations for the adoption of any technical curriculum under flexible teaching conditions—be it on-site, hybrid, or online.
Automated Identification of Sleep Disorder Types Using Triplet Half-Band Filter and Ensemble Machine Learning Techniques with EEG Signals
Authors: Sharma Manish , Tiwari Jainendra , Patel Virendra , Acharya U. Rajendra
Published: June 2021

A sleep disorder is a medical condition that affects an individual’s regular sleeping pattern and routine, hence negatively affecting the individual’s health. The traditional procedures of identifying sleep disorders by clinicians involve questionnaires and polysomnography (PSG), which are subjective, time-consuming, and inconvenient. Hence, an automated sleep disorder identification is required to overcome these limitations. In the proposed study, we have proposed a method using electroencephalogram (EEG) signals for the automated identification of six sleep disorders, namely insomnia, nocturnal frontal lobe epilepsy (NFLE), narcolepsy, rapid eye movement behavior disorder (RBD), periodic leg movement disorder (PLM), and sleep-disordered breathing (SDB). To the best of our belief, this is one of the first studies ever undertaken to identify sleep disorders using EEG signals employing cyclic alternating pattern (CAP) sleep database. After sleep-scoring EEG epochs, we have created eight different data subsets of EEG epochs to develop the proposed model. A novel optimal triplet half-band filter bank (THFB) is used to obtain the subbands of EEG signals. We have extracted Hjorth parameters from subbands of EEG epochs. The selected features are fed to various supervised machine learning algorithms for the automated classification of sleep disorders. Our proposed system has obtained the highest accuracy of 99.2%, 98.2%, 96.2%, 98.3%, 98.8%, and 98.8% for insomnia, narcolepsy, NFLE, PLM, RBD, and SDB classes against normal healthy subjects, respectively, applying ensemble boosted trees classifier. As a result, we have attained the highest accuracy of 91.3% to identify the type of sleep disorder. The proposed method is simple, fast, efficient, and may reduce the challenges faced by medical practitioners during the diagnosis of various sleep disorders accurately in less time at sleep clinics and homes.
Data-Driven Fault Diagnosis for Satellite Control Moment Gyro Assembly with Multiple In-Phase Faults
Authors: Varvani Farahani Hossein , Rahimi Afshin
Published: June 2021

A satellite can only complete its mission successfully when all its subsystems, including the attitude control subsystem, are in healthy condition and work properly. Control moment gyroscope is a type of actuator used in the attitude control subsystems of satellites. Any fault in the control moment gyroscope can cause the satellite mission failure if it is not detected, isolated, and resolved in time. Fault diagnosis provides an opportunity to detect and isolate the occurring faults and, if accompanied by proactive remedial actions, it can avoid failure and improve the satellite reliability. In this paper, an enhanced data-driven fault diagnosis is introduced for fault isolation of multiple in-phase faults of satellite control moment gyroscopes that has not been addressed in the literature before with high accuracy. The proposed method is based on an optimized support vector machine, and the results yield fault predictions with up to 95.6% accuracy. In addition, a sensitivity analysis with regard to noise, missing values, and missing sensors is done. The results show that the proposed model is robust enough to be used in real applications.
Phaseless Characterization of Compact Antenna Test Range via Improved Alternating Projection Algorithm
Authors: Chen Yuqing , Yao Yuan , Zhu Lei , Yu Haiyang , Cheng Xiaohe , Yu Junsheng , Chen Xiaodong
Published: June 2021

A practical compact antenna test range (CATR) requires good quiet zone quality for antenna characterization. This paper addresses the phase profile of the CATR quiet zone from the known intensity pattern of spatial domain and Fourier domain based on a combined alternating projection algorithm. The proposed algorithm is composed of Gerchberg–Saxton (GS) and Hybrid Input–Output (HIO) algorithms and the two algorithms with spatial phase perturbation (SPP) work collaboratively or independently under predesigned conditions. It is observed that the algorithm with random initial phase guess can always converge to an optimal solution by performing a series of hierarchical optimizations of the problem. The numerical results are in good agreement with simulated results in different frequency bands, overcoming the phase retrieval limitation of local convergence in the iterative process. Furthermore, to validate the effectiveness and robustness of the proposed procedure, the related discussions corresponding to different sampling areas in Fourier domain and different signal to noise ratios (SNRs) are given.
Thermal Analysis and Creep Lifetime Prediction Based on the Effectiveness of Thermal Barrier Coating on a Gas Turbine Combustor Liner Using Coupled CFD and FEM Simulation
Authors: Radhakrishnan Kanmaniraja , Park Jun Su
Published: June 2021

Thermal barrier coating (TBC) plays a vital role in the gas turbine combustor liner (CL) to mitigate the internal heat transfer from combustion gas to the CL and enhance the parent material lifetime of the CL. This present study examined the thermal analysis and creep lifetime prediction based on three different TBC thicknesses, 400, 800, and 1200 μm, coated on the inner CL using the coupled computational fluid dynamics/finite element method. The simulation method was divided into three models to minimize the amount of computational work involved. The Eddy Dissipation Model was used in the first model to simulate premixed methane-air combustion, and the wall temperature of the inner CL was obtained. The conjugate heat transfer simulation on the external cooling flows from the rib turbulator, impingement jet, and cross flow, and the wall temperature of the outer CL was obtained in the second model. The thermal analysis was carried out in the third model using three different TBC thicknesses and incorporating the wall data from the first and second model. The effect of increasing TBC thickness shows that the TBC surface temperature was increased. Thereby, the inner CL metal temperature was decreased due to the TBC thickness as well as the material properties of Yttria Stabilized Zirconia, which has low thermal conductivity and a high thermal expansion coefficient. With the increase in TBC thickness, the average temperature difference between the TBC surface and the inner metal surface increased. In contrast, the average temperature difference between the inner and outer metal surfaces remained nearly constant. The von Mises equivalent stress, based on the material property and thermal expansion coefficient, was determined and used to find the creep lifetime of the CL using the Larson–Miller rupture curve for all TBC thickness cases in order to analyze the thermo-structure. Except in the C-channel, the increasing TBC thickness was found to effectively increase the CL lifespan. Furthermore, the case without TBC was compared with the damaged CL with cracks due to thermal stress, which was prevented by increasing TBC thickness shown in this present study.
Planning of Aegean Archipelago Interconnections to the Continental Power System of Greece
Authors: Karystianos Michalis E. , Pitas Charalampos N. , Efstathiou Stamatina P. , Tsili Marina A. , Mantzaris John C. , Leonidaki Eirini A. , Voumvoulakis Emmanouil M. , Sakellaridis Nikos G.
Published: June 2021

This paper presents the results of a thorough planning investigation carried out by the Transmission System Operator (TSO) and the Distribution Network Operator (DNO) in Greece in order to study the interconnections of the Aegean Islands with the mainland power system. The feasibility of interconnecting islands is based on the need for a reliable power supply to isolated and autonomous islands, reducing the operating costs of the power generation system through the withdrawal of high-cost oil-fired generating units, and the further ability to increase renewable energy source (RES) penetration in the country’s energy mix, according to directions imposed by the National Energy and Climate Plan. Among the alternative topologies and interconnection technologies considered, the final selection of the interconnection scheme selected for inclusion in the development plans of TSO and DNO was based on their detailed comparative evaluation, considering financial and other technical criteria.
Lagrangian Vortex Computations of a Four Tidal Turbine Array: An Example Based on the NEPTHYD Layout in the Alderney Race
Authors: Slama Myriam , Choma Bex Camille , Pinon Grégory , Togneri Michael , Evans Iestyn
Published: June 2021

This study investigates the wake interaction of four full-scale three-bladed tidal turbines with different ambient turbulence conditions, in straight and yawed flows. A three-dimensional unsteady Lagrangian Vortex Blob software is used for the numerical simulations of the turbines’ wakes. In order to model the ambient turbulence in the Lagrangian Vortex Method formalism, a Synthetic Eddy Method is used. With this method, turbulent structures are added in the computational domain to generate a velocity field which statistically reproduces any ambient turbulence intensity and integral length scale. The influence of the size of the structures and their density (within the study volume) on the wake of a single turbine is studied. Good agreement is obtained between numerical and experimental results for a high turbulence intensity but too many structures can increase the numerical dissipation and reduce the wake extension. Numerical simulations of the four turbine array with the layout initially proposed for the NEPTHYD pilot farm are then presented. Two ambient turbulence intensities encountered in the Alderney Race and two integral length scales are tested with a straight flow. Finally, the wakes obtained for yawed flows with different angles are presented, highlighting turbine interactions.
Pandemic of Childhood Myopia. Could New Indoor LED Lighting Be Part of the Solution?
Authors: Baeza Moyano David , González-Lezcano Roberto Alonso
Published: June 2021

The existence of a growing myopia pandemic is an unquestionable fact for health authorities around the world. Different possible causes have been put forward over the years, such as a possible genetic origin, the current excess of children’s close-up work compared to previous stages in history, insufficient natural light, or a multifactorial cause. Scientists are looking for different possible solutions to alleviate it, such as a reduction of time or a greater distance for children’s work, the use of drugs, optometric correction methods, surgical procedures, and spending more time outdoors. There is a growing number of articles suggesting insufficient natural light as a possible cause of the increasing levels of childhood myopia around the globe. Technological progress in the world of lighting is making it possible to have more monochromatic LED emission peaks, and because of this, it is possible to create spectral distributions of visible light that increasingly resemble natural light in the visible range. The possibility of creating indoor luminaires that emit throughout the visible spectrum from purple to infrared can now be a reality that could offer a new avenue of research to fight this pandemic.
Quantifying the Potential of Renewable Natural Gas to Support a Reformed Energy Landscape: Estimates for New York State
Authors: Taboada Stephanie , Clark Lori , Lindberg Jake , Tonjes David J. , Mahajan Devinder
Published: June 2021

Public attention to climate change challenges our locked-in fossil fuel-dependent energy sector. Natural gas is replacing other fossil fuels in our energy mix. One way to reduce the greenhouse gas (GHG) impact of fossil natural gas is to replace it with renewable natural gas (RNG). The benefits of utilizing RNG are that it has no climate change impact when combusted and utilized in the same applications as fossil natural gas. RNG can be injected into the gas grid, used as a transportation fuel, or used for heating and electricity generation. Less common applications include utilizing RNG to produce chemicals, such as methanol, dimethyl ether, and ammonia. The GHG impact should be quantified before committing to RNG. This study quantifies the potential production of biogas (i.e., the precursor to RNG) and RNG from agricultural and waste sources in New York State (NYS). It is unique because it is the first study to provide this analysis. The results showed that only about 10% of the state’s resources are used to generate biogas, of which a small fraction is processed to RNG on the only two operational RNG facilities in the state. The impact of incorporating a second renewable substitute for fossil natural gas, “green” hydrogen, is also analyzed. It revealed that injecting RNG and “green” hydrogen gas into the pipeline system can reduce up to 20% of the state’s carbon emissions resulting from fossil natural gas usage, which is a significant GHG reduction. Policy analysis for NYS shows that several state and federal policies support RNG production. However, the value of RNG can be increased 10-fold by applying a similar incentive policy to California’s Low Carbon Fuel Standard (LCFS).
A Review on the Thermochemical Recycling of Waste Tyres to Oil for Automobile Engine Application
Authors: Jahirul Mohammad I. , Hossain Farhad M. , Rasul Mohammad G. , Chowdhury Ashfaque Ahmed
Published: June 2021

Utilising pyrolysis as a waste tyre processing technology has various economic and social advantages, along with the fact that it is an effective conversion method. Despite extensive research and a notable likelihood of success, this technology has not yet seen implementation in industrial and commercial settings. In this review, over 100 recent publications are reviewed and summarised to give attention to the current state of global tyre waste management, pyrolysis technology, and plastic waste conversion into liquid fuel. The study also investigated the suitability of pyrolysis oil for use in diesel engines and provided the results on diesel engine performance and emission characteristics. Most studies show that discarded tyres can yield 40–60% liquid oil with a calorific value of more than 40 MJ/kg, indicating that they are appropriate for direct use as boiler and furnace fuel. It has a low cetane index, as well as high viscosity, density, and aromatic content. According to diesel engine performance and emission studies, the power output and combustion efficiency of tyre pyrolysis oil are equivalent to diesel fuel, but engine emissions (NOX, CO, CO, SOX, and HC) are significantly greater in most circumstances. These findings indicate that tyre pyrolysis oil is not suitable for direct use in commercial automobile engines, but it can be utilised as a fuel additive or combined with other fuels.
Tap Water Quality: Seasonal User Surveys in Poland
Authors: Ober Józef , Karwot Janusz
Published: June 2021

Security of supply of water, which meets the quality parameters specified in applicable standards, is now the basis for the functioning of most societies. In addition to climatic, biological, chemical, and physical hazards, it is worth paying attention to consumers’ subjective perception of the quality of tap water supplied in the area of Poland. The article discusses various activities related to water resources management and analyses the results of an evaluation of selected quality parameters of tap water in Poland. A novelty on a European scale here is an examination of the evaluation of these parameters based on potential seasonal differences (spring, summer, autumn, winter). For the first time in the world literature, PROFIT analysis was used to evaluate selected parameters of tap water quality. The aim of the article was to present a model for the evaluation of the parameters of tap water supplied in different seasons of the year in Poland. Due to the complexity of the research aspects, a mixed-methods research procedure was used in which a literature review was combined with a survey and statistical analysis. For the purpose of the survey, an original survey questionnaire called “Survey of customer opinions on selected parameters of tap water supplied in Poland” was developed especially for this study. The conducted research confirmed the adopted hypothesis that the results of evaluation of selected tap water parameters vary depending on the period (spring, summer, autumn, winter) in Poland. The model developed by means of PROFIT analysis makes it possible to highlight to water suppliers the specific quality parameters in particular seasons of the year (spring, summer, autumn, winter), which may improve the quality of water supplied in Poland and thus, in the long-term perspective, increase the level of satisfaction of water recipients and confidence in drinking tap water in Poland.
Demographic Effects of Severe Fire in Montane Shrubland on Tasmania’s Central Plateau
Authors: Foulkes Judy A. , Prior Lynda D. , Leonard Steven W. J. , Bowman David M. J. S.
Published: June 2021

Australian montane sclerophyll shrubland vegetation is widely considered to be resilient to infrequent severe fire, but this may not be the case in Tasmania. Here, we report on the vegetative and seedling regeneration response of a Tasmanian non-coniferous woody montane shrubland following a severe fire, which burned much of the Great Pine Tier in the Central Plateau Conservation Area during the 2018–2019 fire season when a historically anomalously large area was burned in central Tasmania. Our field survey of a representative area burned by severe crown fire revealed that more than 99% of the shrubland plants were top-killed, with only 5% of the burnt plants resprouting one year following the fire. Such a low resprouting rate means the resilience of the shrubland depends on seedling regeneration from aerial and soil seedbanks or colonization from plants outside the burned area. Woody species’ seedling densities were variable but generally low (25 m−2). The low number of resprouters, and reliance on seedlings for recovery, suggest the shrubland may not be as resilient to fire as mainland Australian montane shrubland, particularly given a warming climate and likely increase in fire frequency.
Physicochemical Properties and Drivers of Liking and Disliking for Cooked Rice Containing Various Types of Processed Whole Wheat
Authors: Lee Da-Been , Kim Mi-Ran , Heo Jeong-Ae , Byeon Yang-Soo , Kim Sang-Sook
Published: June 2021

For utilization of whole wheat (WW) in cooked rice products, WW was processed by four different methods (steeping (S_WW), milling (M_WW), enzymatic treatment (E_WW), and passing through a roll mill (1 mm) (R_WW)). Additionally, the physicochemical properties of cooked rice containing various processed wheat were investigated. The hardness of the cooked rice decreased significantly with R_WW and E_WW compared to WW. As a result of a consumer acceptance test, the cooked rice samples containing M_WW and E_WW with high liking scores frequently included ‘chewiness’ as a reason for liking, and the cooked rice with WW and S_WW was mentioned as being ‘too hard’ as a reason for disliking. The cooked rice with R_WW, which had the lowest liking score, was mentioned as having appearance characteristics such as ‘husk’, ‘clumpy appearance’, and ‘messy appearance’ as reasons for disliking. The overall results of this study suggest the inclusion of M_WW or E_WW with cooked rice considering health-related benefits and consumer acceptability.
Physicochemical and Sensory Assessments in Spain and United States of PGI-Certified Ternera de Navarra vs. Certified Angus Beef
Authors: Beriain María José , Murillo-Arbizu María T. , Insausti Kizkitza , Ibañez Francisco C. , Cord Christine Leick , Carr Tom R.
Published: June 2021

The physicochemical and sensory differences between the PGI-Certified Ternera de Navarra (CTNA) (Spanish origin) and Certified Angus Beef (CAB) (US origin) were assessed in Spain and the USA. To characterize the carcasses, the ribeye areas (REAs), and marbling levels were assessed in both testing places. Twenty striploins per certified beef program were used as study samples. For sensory analysis, the striploins were vacuum packaged and aged for 7 days at 4 °C and 85% RH in each corresponding laboratory. Thereafter, the samples were half cut and frozen. One of the halves was shipped to the other counterpart-testing place. The fat and moisture percentage content, Warner Bratzler Shear Force (WBSF), and total and soluble collagen were tested for all the samples. The CAB carcasses had smaller REAs (p < 0.0001) and exhibited higher marbling levels (p < 0.0001). The CAB striploins had a higher fat content (p < 0.0001) and required lower WBSF (p < 0.05) than the CTNA samples. Trained panelists rated the CAB samples as juicer (p < 0.001), more tender/less tough (p < 0.0001), and more flavorful (p < 0.0001) than the CTNA counterparts. This study shows that beef from both countries had medium-high tenderness, juiciness, and beef flavor scores and very low off-flavor scores. Relevant differences found between the ratings assigned by the Spanish and the US panelists suggest training differences, or difficulties encountered in using the appropriate terminology for defining each sensory attribute. Furthermore, the lack of product knowledge (i.e., consumption habits) may have been another reason for such differences, despite the blind sensory evaluation.
Antilisterial Effect of a Natural Formulation Based on Citrus Extract in Ready-To-Eat Foods
Authors: Ariza Juan José , García-López David , Sánchez-Nieto Esperanza , Guillamón Enrique , Baños Alberto , Martínez-Bueno Manuel
Published: June 2021

Controlling Listeria in food is a major challenge, especially because it can persist for years in food processing plants. The best option to control this pathogen is the implementation of effective cleaning and disinfection procedures that guarantee the safety and quality of the final products. In addition, consumer trends are changing, being more aware of the importance of food safety and demanding natural foods, minimally processed and free of chemical additives. For this reason, the current consumption model is focusing on the development of preservatives of natural origin, from plants or microorganisms. In sum, this study aimed to evaluate the antimicrobial effectiveness of a citrus extract formulation rich in flavonoids against several L. monocytogenes and L. innocua strains, using in vitro test (agar diffusion test, minimum bactericidal concentration (MBC), and time-kill curves) and challenge test in food trials (carne mechada, salami, fresh salmon, lettuce, brine, and mozzarella cheese). The results presented in this work show that citrus extract, at doses of 5 and 10%, had a relevant antimicrobial activity in vitro against the target strains tested. Besides this, citrus extract applied on the surface of food had a significant antilisterial activity, mainly in carne mechada and mozzarella cheese, with reductions of up to eight logarithmic units with respect to the control. These results suggest that citrus extract can be considered a promising tool to improve the hygienic quality of ready-to-eat foods.
Impact of Hydrolyzed Collagen from Defatted Sea Bass Skin on Proliferation and Differentiation of Preosteoblast MC3T3-E1 Cells
Authors: Chotphruethipong Lalita , Binlateh Thunwa , Hutamekalin Pilaiwanwadee , Aluko Rotimi E. , Tepaamorndech Surapun , Zhang Bin , Benjakul Soottawat
Published: June 2021

Osteoporosis is a serious problem affecting health of the elderly. Drugs (bisphosphonates) applied for treatment are often accompanied by adverse side effects. Thus, fish byproduct-derived peptides, particularly hydrolyzed collagen (HC) from defatted sea bass skin, could be a safe source of anti-osteoporosis agents. This study aimed to examine the effects of HC on proliferation and differentiation of preosteoblast cells. HC prepared using papain before Alcalase hydrolysis was determined for molecular weight (MW) distribution. Thereafter, the resulting HC (50–800 µg/mL) was added to the cell. Proliferation, alkaline phosphatase activity (AP-A) and mineralization of cells were investigated. Moreover, the expression of runt-related transcription factor 2 (RUNX2) and the p-Akt/Akt pathway were also determined using Western blot. The results showed that HC had an MW < 3 kDa. HC (50–200 µg/mL) could promote cell proliferation. Nevertheless, HC at 100 µg/mL (HC-100) had enhanced AP-A and increased mineralization during the first 7 days of culture. Moreover, HC-treated cells had higher calcium depositions than the control (p < 0.05). Additionally, cells treated with HC-100 had higher levels of RUNX2 and p-Akt expressions than control (p < 0.05). Therefore, HC could be a promising functional ingredient to promote osteoblast proliferation and differentiation, which could enhance bone strength.