bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantitative Proteomic Characterization of Foreign Body Response towards Silicone Breast Implants Identifies Chronological Disease-Relevant Biomarker Dynamics
Authors: Schoberleitner Ines , Faserl Klaus , Sarg Bettina , Egle Daniel , Brunner Christine , Wolfram Dolores
Published: February 2023

The etiology of exaggerated fibrous capsule formation around silicone mammary implants (SMI) is multifactorial but primarily induced by immune mechanisms towards the foreign material silicone. The aim of this work was to understand the disease progression from implant insertion and immediate tissue damage response reflected in (a) the acute wound proteome and (b) the adsorption of chronic inflammatory wound proteins at implant surfaces. An intraindividual relative quantitation TMT-liquid chromatography–tandem mass spectrometry approach was applied to the profile wound proteome formed around SMI in the first five days post-implantation. Compared to plasma, the acute wound profile resembled a more complex composition comprising plasma-derived and locally differentially expressed proteins (DEPs). DEPs were subjected to a functional enrichment analysis, which revealed the dysregulation of signaling pathways mainly involved in immediate inflammation response and ECM turnover. Moreover, we found time-course variations in protein enrichment immediately post-implantation, which were adsorbed to SMI surfaces after 6–8 months. Characterization of the expander-adhesive proteome by a label-free approach uncovered a long-term adsorbed acute wound and the fibrosis-associated proteome. Our findings propose a wound biomarker panel for the early detection and diagnosis of excessive fibrosis that could potentially broaden insights into the characteristics of fibrotic implant encapsulation.
Progress in Investigational Agents Targeting Serotonin-6 Receptors for the Treatment of Brain Disorders
Authors: Nirogi Ramakrishna , Jayarajan Pradeep , Shinde Anil , Mohammed Abdul Rasheed , Grandhi Venkata Ramalingayya , Benade Vijay , Goyal Vinod Kumar , Abraham Renny , Jasti Venkat , Cummings Jeffrey
Published: February 2023

Serotonin (5-HT) plays an important role in the regulation of several basic functions of the central and peripheral nervous system. Among the 5-HT receptors, serotonin-6 (5-HT6) receptor has been an area of substantial research. 5-HT6 receptor is a G-protein-coupled receptor mediating its effects through diverse signaling pathways. Exceptional features of the receptors fueling drug discovery efforts include unique localization and specific distribution in the brain regions having a role in learning, memory, mood, and behavior, and the affinity of several clinically used psychotropic agents. Although non-clinical data suggest that both agonist and antagonist may have similar behavioral effects, most of the agents that entered clinical evaluation were antagonists. Schizophrenia was the initial target; more recently, cognitive deficits associated with Alzheimer’s disease (AD) or other neurological disorders has been the target for clinically evaluated 5-HT6 receptor antagonists. Several 5-HT6 receptor antagonists (idalopirdine, intepirdine and latrepirdine) showed efficacy in alleviating cognitive deficits associated with AD in the proof-of-concept clinical studies; however, the outcomes of the subsequent phase 3 studies were largely disappointing. The observations from both non-clinical and clinical studies suggest that 5-HT6 receptor antagonists may have a role in the management of neuropsychiatric symptoms in dementia. Masupirdine, a selective 5-HT6 receptor antagonist, reduced agitation/aggression-like behaviors in animal models, and a post hoc analysis of a phase 2 trial suggested potential beneficial effects on agitation/aggression and psychosis in AD. This agent will be assessed in additional trials, and the outcome of the trials will inform the use of 5-HT6 receptor antagonists in the treatment of agitation in dementia of the Alzheimer’s type.
Combination of Experimental and Bioinformatic Approaches for Identification of Immunologically Relevant Protein–Peptide Interactions
Authors: Debeljak Jerneja , Korošec Peter , Šelb Julij , Rijavec Matija , Košnik Mitja , Lunder Mojca
Published: February 2023

Protein–peptide interactions are an essential player in cellular processes and, thus, of great interest as potential therapeutic agents. However, identifying the protein’s interacting surface has been shown to be a challenging task. Here, we present a methodology for protein–peptide interaction identification, implementing phage panning, next-generation sequencing and bioinformatic analysis. One of the uses of this methodology is identification of allergen epitopes, especially suitable for globular inhaled and venom allergens, where their binding capability is determined by the allergen’s conformation, meaning their interaction cannot be properly studied when denatured. A Ph.D. commercial system based on the M13 phage vector was used for the panning process. Utilization of various bioinformatic tools, such as PuLSE, SAROTUP, MEME, Hammock and Pepitope, allowed us to evaluate a large amount of obtained data. Using the described methodology, we identified three peptide clusters representing potential epitopes on the major wasp venom allergen Ves v 5.
Transcutaneous Electrical Cranial-Auricular Acupoint Stimulation Modulating the Brain Functional Connectivity of Mild-to-Moderate Major Depressive Disorder: An fMRI Study Based on Independent Component Analysis
Authors: Liao Lifang , Zhang Liulu , Lv Jun , Liu Yingchun , Fang Jiliang , Rong Peijing , Liu Yong
Published: February 2023

Evidence has shown the roles of taVNS and TECS in improving depression but few studies have explored their synergistic effects on MDD. Therefore, the treatment responsivity and neurological effects of TECAS were investigated and compared to escitalopram, a commonly used medication for depression. Fifty patients with mild-to-moderate MDD (29 in the TECAS group and 21 in another) and 49 demographically matched healthy controls were recruited. After an eight-week treatment, the outcomes of TECAS and escitalopram were evaluated by the effective rate and reduction rate based on the Montgomery–Asberg Depression Rating Scale, Hamilton Depression Rating Scale, and Hamilton Anxiety Rating Scale. Altered brain networks were analyzed between pre- and post-treatment using independent component analysis. There was no significant difference in clinical scales between TECAS and escitalopram but these were significantly decreased after each treatment. Both treatments modulated connectivity of the default mode network (DMN), dorsal attention network (DAN), right frontoparietal network (RFPN), and primary visual network (PVN), and the decreased PVN–RFPN connectivity might be the common brain mechanism. However, there was increased DMN–RFPN and DMN–DAN connectivity after TECAS, while it decreased in escitalopram. In conclusion, TECAS could relieve symptoms of depression similarly to escitalopram but induces different changes in brain networks.
Investigation into Viscoelastic Properties of Fiber-Reinforced Asphalt Composite Concrete Based on the Burgers Model
Authors: Huang Chunshui , Gao Danying , Meng Tong , Yang Changde
Published: February 2023

Asphalt composite concrete pavement is one of the common pavement forms in China. However, due to the influence of design, materials, construction quality, and other aspects, asphalt composite concrete pavement develops various degrees of cracks after being put into use, which affects the service performance and life of asphalt pavement. The Burgers model is used to examine the effects of fiber-volume-fraction and length–diameter-ratio on the viscoelastic mechanical behavior model parameters and viscoelastic properties of asphalt composite concrete through the bend test for creep of polyester fiber asphalt composite concrete beam. The findings indicate that the fiber’s ability to control asphalt composite concrete bending creep deformation increases initially and subsequently diminishes as fiber-volume-fraction and length–diameter-ratio increase. Fiber-volume-fraction and length–diameter-ratio effects can be fully reflected by fiber amount characteristics. A viscoelastic mechanical behavior model of fiber-reinforced asphalt composite concrete is developed on this foundation while taking into account the influence of fiber amount characteristic factors. Theoretical study and practical research indicate that the ideal fiber volume ratio of polyester fiber asphalt composite concrete is 0.35 percent, the ideal length-to-diameter ratio is 324, and the ideal fiber amount characteristic parameter is 1.13. The test results can provide a certain reference value for the improvement of the long-term durability of fiber asphalt composite concrete pavement of road engineering.
Pharmacological Inhibition of Membrane Signaling Mechanisms Reduces the Invasiveness of U87-MG and U251-MG Glioblastoma Cells In Vitro
Authors: Varricchio Alanah , Khan Sidra , Price Zoe K. , Davis Rohan A. , Ramesh Sunita A. , Yool Andrea J.
Published: February 2023

Impairing the motility of glioblastoma multiforme (GBM) cells is a compelling goal for new approaches to manage this highly invasive and rapidly lethal human brain cancer. Work here characterized an array of pharmacological inhibitors of membrane ion and water channels, alone and in combination, as tools for restraining glioblastoma spread in human GBM cell lines U87-MG and U251-MG. Aquaporins, AMPA glutamate receptors, and ion channel classes (shown to be upregulated in human GBM at the transcript level and linked to mechanisms of motility in other cell types) were selected as pharmacological targets for analyses. Effective compounds reduced the transwell invasiveness of U87-MG and U251-MG glioblastoma cells by 20–80% as compared with controls, without cytotoxicity. The compounds and doses used were: AqB013 (14 μM); nifedipine (25 µM); amiloride (10 µM); apamin (10 µM); 4-aminopyridine (250 µM); and CNQX (6-cyano-7-nitroquinoxaline-2,3-dione; 30 µM). Invasiveness was quantified in vitro across transwell filter chambers layered with extracellular matrix. Co-application of each of the ion channel agents with the water channel inhibitor AqB013 augmented the inhibition of invasion (20 to 50% greater than either agent alone). The motility impairment achieved by co-application of pharmacological agents differed between the GBM proneural-like subtype U87-MG and classical-like subtype U251-MG, showing patterns consistent with relative levels of target channel expression (Human Protein Atlas database). In addition, two compounds, xanthurenic acid and caelestine C (from the Davis Open Access Natural Product-based Library, Griffith University QLD), were discovered to block invasion at micromolar doses in both GBM lines (IC50 values from 0.03 to 1 µM), without cytotoxicity, as measured by full mitochondrial activity under conditions matching those in transwell assays and by normal growth in spheroid assays. Mechanisms of action of these agents based on published work are likely to involve modulation of glutamatergic receptor signaling. Treating glioblastoma by the concurrent inhibition of multiple channel targets could be a powerful approach for slowing invasive cell spread without cytotoxic side effects, potentially enhancing the effectiveness of clinical interventions focused on eradicating primary tumors.
Combined Inhibition of IAPs and WEE1 Enhances TNFα- and Radiation-Induced Cell Death in Head and Neck Squamous Carcinoma
Authors: Toni Tiffany , Viswanathan Ramya , Robbins Yvette , Gunti Sreenivasulu , Yang Xinping , Huynh Angel , Cheng Hui , Sowers Anastasia , Mitchell James , Allen Clint , Morgan Ethan , Van Waes Carter
Published: February 2023

Head and neck squamous cell carcinoma (HNSCC) remains a prevalent diagnosis with current treatment options that include radiotherapy and immune-mediated therapies, in which tumor necrosis factor-α (TNFα) is a key mediator of cytotoxicity. However, HNSCC and other cancers often display TNFα resistance due to activation of the canonical IKK–NFκB/RELA pathway, which is activated by, and induces expression of, cellular inhibitors of apoptosis proteins (cIAPs). Our previous studies have demonstrated that the IAP inhibitor birinapant sensitized HNSCC to TNFα-dependent cell death in vitro and radiotherapy in vivo. Furthermore, we recently demonstrated that the inhibition of the G2/M checkpoint kinase WEE1 also sensitized HNSCC cells to TNFα-dependent cell death, due to the inhibition of the pro-survival IKK-NFκB/RELA complex. Given these observations, we hypothesized that dual-antagonist therapy targeting both IAP and WEE1 proteins may have the potential to synergistically sensitize HNSCC to TNFα-dependent cell death. Using the IAP inhibitor birinapant and the WEE1 inhibitor AZD1775, we show that combination treatment reduced cell viability, proliferation and survival when compared with individual treatment. Furthermore, combination treatment enhanced the sensitivity of HNSCC cells to TNFα-induced cytotoxicity via the induction of apoptosis and DNA damage. Additionally, birinapant and AZD1775 combination treatment decreased cell proliferation and survival in combination with radiotherapy, a critical source of TNFα. These results support further investigation of IAP and WEE1 inhibitor combinations in preclinical and clinical studies in HNSCC.
Identification of T Cell Receptors Targeting a Neoantigen Derived from Recurrently Mutated FGFR3
Authors: Tate Tomohiro , Matsumoto Saki , Nemoto Kensaku , Leisegang Matthias , Nagayama Satoshi , Obama Kazutaka , Nakamura Yusuke , Kiyotani Kazuma
Published: February 2023

Immunotherapies, including immune checkpoint blockades, play a critically important role in cancer treatments. For immunotherapies, neoantigens, which are generated by somatic mutations in cancer cells, are thought to be good targets due to their tumor specificity. Because neoantigens are unique in individual cancers, it is challenging to develop personalized immunotherapy targeting neoantigens. In this study, we screened "shared neoantigens", which are specific types of neoantigens derived from mutations observed commonly in a subset of cancer patients. Using exome sequencing data in the Cancer Genome Atlas (TCGA), we predicted shared neoantigen peptides and performed in vitro screening of shared neoantigen-reactive CD8+ T cells using peripheral blood from healthy donors. We examined the functional activity of neoantigen-specific T cell receptors (TCRs) by generating TCR-engineered T cells. Among the predicted shared neoantigens from TCGA data, we found that the mutated FGFR3Y373C peptide induced antigen-specific CD8+ T cells from the donor with HLA-A*02:06 via an ELISPOT assay. Subsequently, we obtained FGFR3Y373C-specific CD8+ T cell clones and identified two different sets of TCRs specifically reactive to FGFR3Y373C. We found that the TCR-engineered T cells expressing FGFR3Y373C-specific TCRs recognized the mutated FGFR3Y373C peptide but not the corresponding wild-type peptide. These two FGFR3Y373C-specific TCR-engineered T cells showed cytotoxic activity against mutated FGFR3Y373C-loaded cells. These results imply the possibility of strategies of immunotherapies targeting shared neoantigens, including cancer vaccines and TCR-engineered T cell therapies.
Bevacizumab as Single Agent in Children and Teenagers with Optic Pathway Glioma
Authors: Calò Pierluigi , Pianton Nicolas , Basle Alexandre , Vasiljevic Alexandre , Barritault Marc , Beuriat Pierre Aurélien , Faure-Conter Cécile , Leblond Pierre
Published: February 2023

This is a retrospective study conducted on patients with OPG, aged less than 19 years, treated with bevacizumab as a single agent, since 2010 at IHOPe (Institute of Pediatric Hematology and Oncology). Efficacy of the treatment was evaluated on the tumor response rate on MRI with a centralized review basing upon RAPNO criteria and with visual assessment basing upon a 0.2 log change in the logMAR scale. Thirty-one patients with OPG have been included. From a radiological point of view, best anytime responses were: 1 major response, 6 partial responses, 7 minor responses and 14 stable diseases; achieving disease control in 28 (96%) out of 29 patients. Ophthalmological response was evaluated in 25 patients and disease control was achieved in 22 (88%) out of 25, with 14 steady states and 8 significant improvements. Among patients treated with chemotherapy after the bevacizumab course, nine relapsed and have been retreated with objective responses. Bevacizumab used as single agent seems effective in children and adolescents with OPG. Our work paves the way for a phase II study in which bevacizumab alone could be used as frontline therapy.
Increased Expression Levels of Netrin-1 in Visceral Adipose Tissue during Obesity Favour Colon Cancer Cell Migration
Authors: Mentxaka Amaia , Gómez-Ambrosi Javier , Neira Gabriela , Ramírez Beatriz , Becerril Sara , Rodríguez Amaia , Valentí Víctor , Moncada Rafael , Baixauli Jorge , Burrell María A. , Silva Camilo , Claro Vasco , Ferro Albert , Catalán Victoria , Frühbeck Gema
Published: February 2023

Netrin (NTN)-1, an extracellular matrix protein with a crucial role in inflammation, is dysregulated during obesity (OB) and influences colon cancer (CC) progression. To decipher the mechanisms underlying CC development during obesity, we examined the expression of NTN1 and its receptors in the visceral adipose tissue (VAT) of 74 (25 normal weight (NW)) (16 with CC) and 49 patients with OB (12 with CC). We also evaluated the effect of caloric restriction (CR) on the gene expression levels of Ntn1 and its receptors in the colon from a rat model fed a normal diet. The impact of adipocyte-conditioned media (ACM) from patients with OB and NTN-1 was assessed on the expression levels of neogenin 1(NEO1), deleted in colorectal carcinomas (DCC) and uncoordinated-5 homolog B (UNC5B) in Caco-2 and HT-29 human colorectal cell lines, as well as on Caco-2 cell migration. Increased NTN1 and NEO1 mRNA levels in VAT were due to OB (p < 0.05) and CC (p < 0.001). In addition, an upregulation in the expression levels of DCC and UNC5B in patients with CC (p < 0.01 and p < 0.05, respectively) was observed. Decreased (p < 0.01) Ntn1 levels in the colon from rats submitted to CR were found. In vitro experiments showed that ACM increased DCC (p < 0.05) and NEO1 (p < 0.01) mRNA levels in HT-29 and Caco-2 cell lines, respectively, while UNC5B decreased (p < 0.01) in HT-29. The treatment with NTN-1 increased (p < 0.05) NEO1 mRNA levels in HT-29 cells and DCC (p < 0.05) in both cell lines. Finally, we revealed a potent migratory effect of ACM and NTN-1 on Caco-2 cells. Collectively, these findings point to increased NTN-1 during OB and CC fuelling cancer progression and exerting a strong migratory effect on colon cancer cells.
Innovative Electrocatalysts for Fuel Cell and Battery Applications
Authors: Vecchio Carmelo Lo
Published: February 2023

The development of sustainable energy systems is essential to hinder global warming and environmental pollution emergencies [...]
Similarities and Differences between Site-Selective Acylation and Phosphorylation of Amphiphilic Diols, Promoted by Nucleophilic Organocatalysts Decorated with Outer-Sphere Appendages
Authors: Fleischer Or , Targel Tom , Saady Fatma , Portnoy Moshe
Published: February 2023

We demonstrated recently that organocatalysts, based on a nucleophilic core (N-alkylimidazole or 4-aminopyridine) and decorated with an extensive secondary-sphere envelope (connected to the core through a benzyl substituent), strongly affect the site selectivity in acylation and phosphorylation of amphiphilic diols, sometimes entirely overriding the innate predisposition of the substrate. Preliminary studies pointed out that, despite some similarities, there are differences between the two transformations, regarding the influence of various catalyst features on the selectivity. To fully elucidate this, extended families of organocatalysts of the said design were examined, activity- and selectivity-wise, in the abovementioned transformations of model alcohol and amphiphilic diol substrates. A comparison of the influence of the catalyst design on the two reactions revealed that while the inductive electron donation of the o,o-dialkoxybenzyl substituent of the core, combined with the catalytic intermediate-stabilizing influence of some of the secondary-sphere components, causes an increase in the catalyst activity in both reactions and in the site selectivity in phosphorylation, its effect on the site selectivity in acylation is opposite. On the other hand, the lipophilicity of the secondary-sphere appendages improves the apolar site-favoring selectivity in both reactions. Thus, both factors work in concert in phosphorylation, but in opposite directions in acylation.
Up-Regulation of Astrocytic Fgfr4 Expression in Adult Mice after Spinal Cord Injury
Authors: Bringuier Claire Mathilde , Noristani Harun Najib , Perez Jean-Christophe , Cardoso Maida , Goze-Bac Christophe , Gerber Yannick Nicolas , Perrin Florence Evelyne
Published: February 2023

Spinal cord injury (SCI) leads to persistent neurological deficits without available curative treatment. After SCI astrocytes within the lesion vicinity become reactive, these undergo major morphological, and molecular transformations. Previously, we reported that following SCI, over 10% of resident astrocytes surrounding the lesion spontaneously transdifferentiate towards a neuronal phenotype. Moreover, this conversion is associated with an increased expression of fibroblast growth factor receptor 4 (Fgfr4), a neural stem cell marker, in astrocytes. Here, we evaluate the therapeutic potential of gene therapy upon Fgfr4 over-expression in mature astrocytes following SCI in adult mice. We found that Fgfr4 over-expression in astrocytes immediately after SCI improves motor function recovery; however, it may display sexual dimorphism. Improved functional recovery is associated with a decrease in spinal cord lesion volume and reduced glial reactivity. Cell-specific transcriptomic profiling revealed concomitant downregulation of Notch signaling, and up-regulation of neurogenic pathways in converting astrocytes. Our findings suggest that gene therapy targeting Fgfr4 over-expression in astrocytes after injury is a feasible therapeutic approach to improve recovery following traumatism of the spinal cord. Moreover, we stress that a sex-dependent response to astrocytic modulation should be considered for the development of effective translational strategies in other neurological disorders.
Urinary Proteome Differences in Patients with Type 2 Diabetes Pre and Post Liraglutide Treatment
Authors: Rafiullah Mohamed , Benabdelkamel Hicham , Masood Afshan , Ekhzaimy Aishah A. , Musambil Mohthash , Joy Salini Scaria , Alfadda Assim A.
Published: February 2023

Diabetes mellitus is a chronic multisystem disease with a high global prevalence. The glucagon-like peptide-1 (GLP-1) receptor agonist liraglutide is known to lower glucose levels and reduce weight. However, the mechanisms underlying the benefits of liraglutide treatment in patients with type 2 diabetes mellitus (T2DM) remain unclear. Twelve male patients with T2DM (pre and post liraglutide treatment) and HbA1c between 8% and 11% were recruited. In the present study, a two-dimensional difference gel electrophoresis (2D-DIGE) matrix-assisted laser desorption/ionization-time of flight (MALDI TOF) mass spectrometric approach combined with bioinformatics and network pathway analysis was used to explore the urine proteomic profile. The mean age of the patients was 52.4 ± 7.5 years. After treatment with liraglutide, a statistically significant change (p < 0.006) was observed in HbA1c with no significant changes in body weight or markers of dyslipidemia. Two-dimensional difference gel electrophoresis identified significant changes (≥1.5-fold change, ANOVA, p ≤ 0.05) in 32 proteins (4 down- and 28 upregulated) in liraglutide post treatment compared to the pre-treatment state. Albumin, serotransferrin, metallothionein-2 (MT-2), and keratins K1 and K10 were found to be upregulated after liraglutide treatment. The patients showed significant improvement in glycemic control after the 12-week treatment with liraglutide. The renoprotective effect of liraglutide may be linked to the increased urinary abundance of MT-2 and the decreased abundance of zinc alpha 2-glycoprotein (ZAG) and Alpha-1 antitrypsin (α1-AT). More studies are needed to elucidate the molecular mechanisms behind the renoprotective effects of liraglutide.
Population Survey Combined with Genomic-Wide Genetic Variation Unravels the Endangered Status of Quercus gilva
Authors: Song Yi-Gang , Wang Tian-Rui , Lu Zi-Jia , Ge Bin-Jie , Zhong Xin , Li Xiao-Chen , Jin Dong-Mei , Yuan Quan , Li Yu , Kang Yi-Xin , Ning Xin , Zheng Si-Si , Yi Li-Ta , Dai Xi-Ling , Cao Jian-Guo , Lee Jung-Hyun , Kozlowski Gregor
Published: February 2023

Since the Anthropocene, biodiversity loss owing to human activity and climate change has worsened. Quercus gilva is an evergreen oak species native to China, Japan, and South Korea and is threatened by a long history of human impact. The purpose of this study was to (1) reassess the threatened category of Q. gilva based on a detailed survey, and (2) identify the genetic structure and diversity of Q. gilva based on genomic data. First, we conducted a detailed survey of the populations in China. Second, we collated all the literature and information. Finally, genome-wide genetic variation was analyzed based on 65 individuals from 22 populations. We found that Q. gilva has suffered rapid population decline, and at present, most populations are very small. The evolutionary path of Q. gilva was from the southwest to east of China and then to Japan and South Korea. Quercus gilva showed no distinct genetic structure and had a relatively low genetic diversity. Among the 22 populations, most populations in southwestern China, South Korea, and Japan had high genetic diversity. The populations in Jingning (Zhejiang province; ZJN), Wuyuan (Jinaxi province; JWY), and Zherong (Fujian province; FZR) suffered a strong bottleneck. In conclusion, Q. gilva is an endangered species native to East Asia. Because of the very low genetic diversity of Q. gilva and most populations are small, we need to (1) strengthen the protection of this species, (2) conduct conservation actions with in-situ reinforcement populations, and (3) select populations with high genetic diversity as provenances for afforestation efforts. Finally, we suggest that in the future, genetic diversity should be considered as the sixth criterion for IUCN to evaluate the threatened category.
Focal Choroidal Excavation: Epidemiology, Clinical Characteristics and Multimodal Imaging Findings
Authors: Szabelska Paulina , Mędrzycka Justyna , Brydak-Godowska Joanna , Różycki Radosław , Gołębiewska Joanna
Published: February 2023

Background: Focal choroidal excavation (FCE) is one of the pachychoroid spectrum diseases. It may be an isolated lesion or associated with other ophthalmological disorders. The aim of the study was to present the epidemiology, clinical features and multimodal imaging findings in FCE. Methods: This is a case series of 14 consecutive patients with a diagnosis of FCE, confirmed by multimodal imaging, from a review of the 5076 optical coherence tomography (OCT) scans in 2538 patients. Choroidal thickness (CT) was measured under the fovea and in the area of maximum choroidal thickening in the affected eye and under the fovea in the fellow eye. Results: The mean age of the subjects was 40 ± 13.58 years. FCE occurred unilaterally and was an isolated lesion in all cases. The fellow eye did not show any macular pathology in all patients. Twelve eyes presented conforming FCEs and two non–conforming FCEs. In 79% of cases, FCE was subfoveal. The mean maximum CT was 390 μm in the affected eye with the presence of pachyvessels. A total of 13 patients were asymptomatic, while one patient reported a visual disturbance due to neovascularization secondary to FCE. Of all the multimodal imaging techniques, optical coherence tomography (OCT) provided the most important data in the diagnosis of FCE. Conclusions: Our study confirmed that FCE is a rare ocular condition, but it may be more common in Caucasian population than previously known. Multimodal imaging methods, mainly OCT, are crucial in FCE diagnostics. Further studies are needed to expand the available knowledge about its etiology and clinical course.
Building Multivariate Time-Varying Smooth Transition Correlation GARCH Models, with an Application to the Four Largest Australian Banks
Authors: Hall Anthony D. , Silvennoinen Annastiina , Teräsvirta Timo
Published: February 2023

This paper proposes a methodology for building Multivariate Time-Varying STCC–GARCH models. The novel contributions in this area are the specification tests related to the correlation component, the extension of the general model to allow for additional correlation regimes, and a detailed exposition of the systematic, improved modelling cycle required for such nonlinear models. There is an R-package that includes the steps in the modelling cycle. Simulations demonstrate the robustness of the recommended model building approach. The modelling cycle is illustrated using daily return series for Australia’s four largest banks.
Characteristics of the Board of Directors and Corporate Financial Performance—Empirical Evidence
Authors: Nguyen Van Chien , Huynh Thi Ngoc Thuan
Published: February 2023

The objectives of the research are to investigate the characteristics of the Board of Directors on the financial performance of the enterprise using sample data from 52 construction and real estate enterprises listed on the Vietnam stock exchange in the period 2006–2020. Using typical regression methods such as pooled OLS, FEM, REM, and assessing the defects of the research model, the FGLS method is selected. At the same time, due to the existence of endogenous phenomena and the nature of interdependence among enterprises in Vietnam, research using the instrumental variables two-step generalized method of moments (IV-GMM) is conducted in order to correct for cross-sectional dependence, autocorrelation, endogeneity, and heteroskedasticity in the analysis. Research results suggest that board size, female board members, meeting frequency, and board members’ education have a positive influence on financial performance. Moreover, as the independence of the Board of Directors increases, the business efficiency decreases. The research also found a positive relationship of tangible fixed assets, and a negative relationship between capital structure choice, firm size, and corporate financial performance. Finally, we propose some implications for enhancing the financial performance of Vietnamese firms.
Competitiveness Analysis and Factors Affecting Indonesian Cinnamon Exports
Authors: Rochdiani Dini , Wulandari Eliana
Published: February 2023

Cinnamon is one of the plantation spice commodities that contributes to the Indonesian economy. Indonesia is the largest exporter country in the world regarding the total export volume of cinnamon, but regarding the total value of exports, Indonesia comes in second after Sri Lanka. This study aims to analyze the comparative competitiveness of Indonesian cinnamon among major competitors in the United States’ market and to analyze the factors that influence the competitiveness of Indonesian cinnamon exports in the United States. The data used in this study comprise secondary data from 2007 to 2021 obtained from various data sources. The revealed comparative advantage method was used in a comparative competitiveness analysis. Moreover, the factors that affect the competitiveness of Indonesian cinnamon exports in the United States were analyzed using the ordinary least squares method. The results of the analysis show that Indonesian cinnamon is competitive in the United States’ market, but Sri Lanka’s cinnamon exports have stronger competitiveness than Indonesia’s in the United States’ market. The results of the ordinary least squares method show that the exchange rate of the IDR (rupiah) against the USD, the volume of Indonesian cinnamon exports, the GDP per capita of the USA and the value of cinnamon exports to the USA have a significant effect on the competitiveness of Indonesian cinnamon exports to the United States.
The Influence of the Trump Era on Sustaining Whiteness through Imperialist Reclamation on College Campuses: How Undocumented Students Experience the Normalization of Racist Nativism
Authors: Vigil Darsella , Muñoz Susana M.
Published: February 2023

On 5 September 2017, the Trump administration decided to rescind the Deferred Action for Childhood Action (DACA) policy, impacting over 800,000 recipients, and more colleges and universities witnessed a heightened sense of emboldened racism in college environments. This paper draws from focus groups with undocumented college students on how the Trump era influenced campus climate. We found that imperialist reclamation of whiteness acts targeting undocumented students was commonplace on college campuses, ultimately impacting students’ behavior and academic engagement in and outside the classroom.
Stakeholder Views of Epilepsy in the Biology Classroom: A Qualitative Exploratory Pilot Study
Authors: Tschudnig Manuel , Sommer Martina , Simon Uwe K.
Published: February 2023

While knowledge, attitudes, and practices towards epilepsy have been extensively investigated, studies that focus on recommendations for teaching units about epilepsy are scarce. To gain an understanding of stakeholders’ views on the content and structure of such units and their implications for educational practice, interviews with teachers (n = 4), first aid instructors (n = 4), representatives of epilepsy-related associations (n = 3), neurologists (n = 3), and people with epilepsy (n = 3) were conducted during a qualitative pilot study from May 2020 to August 2020. Transcripts were independently coded by two raters using MAXQDA, reaching a final coding agreement of 92.15%. While experiences with epilepsy vary, almost all interviewees agreed that teaching about epilepsy in school could improve the quality of life of people with epilepsy. A well-designed teaching unit would be welcomed by most stakeholders. Neuronal processes and first aid measures were considered to be the most important topics. Having students with epilepsy in the classroom, enough time for preparation and implementation and not creating anxieties in students were anticipated challenges while teaching. Generally, basic information in (pre-service)-teacher education, cooperation with external experts, and open communication with students with epilepsy and their parents were recommended. Basic knowledge about epilepsy and proper seizure management should also be included in pre-service teacher education. Based on these findings, teaching units on specific aspects of epilepsy should be integrated in classrooms as well as in pre-service teacher education. However, affected students with epilepsy need to be consulted beforehand.
Spatial Path Smoothing for Car-like Robots Using Corridor-Based Quadratic Optimization
Authors: Lu Yongkang , Wu Yuanqing , Zhong Wenjian , Li Yanzhou , Chen Meng
Published: February 2023

The global planning module of car-like robots usually plans a coarse spatial path, which may be non-smooth and kinematically infeasible for car-like robots. This study proposes an efficient spatial path smoothing approach, which is capable of optimizing a rough spatial path to be a high quality one. Two novel designs contribute to the proposed approach. One is a direct corridor construction method that provides an optimization region for path optimization. Based on a redefined path representation in the generated corridor, the second one is a core spatial path optimization method, where the optimization problem is formulated as a multi-objective quadratic programming (QP) with corridor and maximum-curvature constraints. Meanwhile, integrating a two-steps strategy into an optimization process yields a good trade-off between efficiency and quality. Experiment results validate that the proposed approach has the ability to online generate a high quality path.
Diversity Pertaining to the Attenuation of the RF Disturbance Suppression Power Line Filters
Authors: Borecki Michał , Sroka Jan
Published: February 2023

Filters for the suppression of the RF disturbances in power lines are passive; wideband stop filters attenuating common as well as differential disturbances should be insensitive to the variation in the impedance at the input and the output. The design and verification process of such filters differs entirely from that used for the signal RF filters. In the document CISPR 17, entitled “Methods of measurement of the suppression characteristics of passive EMC filtering devices”, two definitions of the attenuation of the common and differential modes are presented. The first is simply direct measurement, and the second is based on a calculation using the single-ended S-parameters of the multiport. These two definitions are the source of diversity. In this paper, we hypothesize that the attenuations achieved with these two methods are identical only if the filter is in perfect balance. Consequently, different attenuations can be achieved. The requirement of balance can be violated due to the tolerances of the components used in the filter. The paper begins with a simple unbalanced four-port, for which analytical formulas are derived. It is also shown that an approach with single-ended S-parameters enables the calculation of the mode conversion between the input and the output, which is called the conversion loss. This is the measure of the grade of the imbalance. Thereafter, the common-mode attenuation of an exemplary RF suppression filter is derived with the presented methods. Additionally, the conversion loss of the filter is calculated. The comparison of the conversion loss with both attenuations is satisfactory, proving the hypothesis formulated in the paper.
RETRACTED: Ground Risk Estimation of Unmanned Aerial Vehicles Based on Probability Approximation for Impact Positions with Multi-Uncertainties
Authors: Liu Yang , Zhu Yuanjun , Wang Zhi , Zhang Xuejun , Li Yan
Published: February 2023

In this paper, a methodology to assess ground risk with multi-uncertainties is introduced, which is associated with a major unmanned aerial vehicle (UAV) in-flight incident. In the assessment model, random factors are taken into account including uncertainty in the drag force, uncertainty in the UAV velocity, and the random effects of local wind. The probability distribution of impact positions is first estimated by using a second-order drag model with probabilistic assumptions regarding the least well-known parameters. Then, an approach for modeling and estimating the ground risks is presented, in which the ground casualties are set as the safety index. In the multifactor risk estimation model, ground casualty areas covered by the UAVs’ debris are determined. Correspondingly, the probability of fatal injuries to people is derived by addressing the protection effects, impact energy, and energy threshold a person can sustain. Further, four kinds of sheltering effects are defined. Finally, the affected area on the ground is partitioned into six zones, taking into consideration the density and distribution of the local population. Case studies are conducted for fixed-wing and rotary-wing UAVs. Risk levels on the ground are obtained and compared with the widely accepted target safety level of manned aircrafts.
Predicting Terrestrial Heat Flow in North China Using Multiple Geological and Geophysical Datasets Based on Machine Learning Method
Authors: Xu Shan , Ni Chang , Hu Xiangyun
Published: February 2023

Geothermal heat flow is an essential parameter for the exploration of geothermal energy. The cost is often prohibitive if dense heat flow measurements are arranged in the study area. Regardless, an increase in the limited and sparse heat flow observation points is needed to study the regional geothermal setting. This research is significant in order to provide a new reliable map of terrestrial heat flow for the subsequent development of geothermal resources. The Gradient Boosted Regression Tree (GBRT) prediction model used in this paper is devoted to solving the problem of an insufficient number of heat flow observations in North China. It considers the geological and geophysical information in the region by training the sample data using 12 kinds of geological and geophysical features. Finally, a robust GBRT prediction model was obtained. The performance of the GBRT method was evaluated by comparing it with the kriging interpolation, the minimum curvature interpolation, and the 3D interpolation algorithm through the prediction performance analysis. Based on the GBRT prediction model, a new heat flow map with a resolution of 0.25°×0.25° was proposed, which depicted the terrestrial heat flow distribution in the study area in a more detailed and reasonable way than the interpolation results. The high heat flow values were mostly concentrated in the northeastern boundary of the Tibet Plateau, with a few scattered and small-scale high heat flow areas in the southeastern part of the North China Craton (NCC) adjacent to the Pacific Ocean. The low heat flow values were mainly resolved in the northern part of the Trans-North China Orogenic belt (TNCO) and the southmost part of the NCC. By comparing the predicted heat flow map with the plate tectonics, the olivine-Mg#, and the hot spring distribution in North China, we found that the GBRT could obtain a reliable result under the constraint of geological and geophysical information in regions with scarce and unevenly distributed heat flow observations.
Research on the Optimal Design of Seasonal Time-of-Use Tariff Based on the Price Elasticity of Electricity Demand
Authors: Xue Wanlei , Zhao Xin , Li Yan , Mu Ying , Tan Haisheng , Jia Yixin , Wang Xuejie , Zhao Huiru , Zhao Yihang
Published: February 2023

Building a new power system with renewable energy as its main component is a key measure proposed by China to address the climate change problem. Strengthening demand-side management (DSM) is an important way to promote the development of a new power system. As an important economic incentive measure in DSM, the current TOU tariff is faced with the problem of a weak incentive effect due to the small tariff difference between the peak and valley periods. Against this background, a novel hybrid three-stage seasonal TOU tariff optimization model is proposed in this paper. First, the K-means++ algorithm is adopted to select the typical days of the four seasons through load curve clustering. Then, the price elasticity of the electricity demand model is constructed to calculate the self-elasticity and cross-elasticity in four seasons. Finally, the seasonal TOU tariff optimization model is constructed to determine the optimal TOU tariff. Through the proposed model, the tariff in the peak period has increased by 8.06–15.39%, and the tariff in the valley period has decreased by 18.48–27.95%. The result shows that the load in the peak period has decreased by 4.03–8.02% and the load in the valley period has increased by 6.41–9.75% through the proposed model.
Energy Trading Strategy of Distributed Energy Resources Aggregator in Day-Ahead Market Considering Risk Preference Behaviors
Authors: Dong Jun , Dou Xihao , Liu Dongran , Bao Aruhan , Wang Dongxue , Zhang Yunzhou
Published: February 2023

Distributed energy resources aggregators (DERAs) are permitted to participate in regional wholesale markets in many counties. At present, new market players such as aggregators participate in China’s power market transactions. However, studies related to market trading strategy have mostly focused on centralized wind power and PV generation units. Few studies have been conducted on the decision-making strategies for DERAs in China’s power market. This paper proposes an auxiliary decision-making model for distributed energy systems to participate in the day-ahead market with more reasonable trading strategies. Firstly, the Gaussian mixture model (GMM) is used to deal with the uncertainties of wind power and photovoltaic (PV) output in the distributed energy system. Secondly, the information gap decision theory (IGDT) is used to deal with the uncertainty of price fluctuations in the spot electricity market. Thirdly, according to the different risk preferences of the DERAs facing market price fluctuation, the robust decision model and opportunity decision-making model in the day-ahead market are constructed, respectively. Finally, to deal with the irrational behavior of the DERAs’ perception of “gain” and “loss” with market risks in China’s two-tier market environment, the prospect theory and the marine predator’s algorithm (MPA) are employed to obtain a day-ahead trading decision scheme for DERA. The analyses show that RDES with robust preference can withstand greater price volatility in the day-ahead market; they will reduce the bidding expectations and increase the system operating cost to improve the achievability of the expected revenue. However, DERAs under the opportunity strategy is more inclined to sell electricity to the market and offset system operating costs with revenue. The proposed model can provide strategic reference for DERAs with different risk preferences to bid in day-ahead market and can improve the level of aggregators’ participation in electricity trading.
Potential of Beekeeping to Support the Livelihood, Economy, Society, and Environment of Indonesia
Authors: Harianja Alfonsus Hasudungan , Adalina Yelin , Pasaribu Gunawan , Winarni Ina , Maharani Rizki , Fernandes Andrian , Saragih Grace Serepina , Fauzi Ridwan , Tampubolon Agustinus Panusunan , Njurumana Gerson Ndawa , Sukito Agus , Aswandi Aswandi , Kholibrina Cut Rizlani , Siswadi Siswadi , Kurniawan Hery , Hidayat Muhamad Yusup , Wahyuni Resti , Koeslulat Ermi Erene , Heryanto Raden Bambang , Basuki Tony , Da Silva Helena , Ngongo Yohanis , deRosari Bernard , Waluyo Totok Kartono , Turjaman Maman , Prabawa Sigit Baktya , Kuspradini Harlinda
Published: February 2023

The management of natural resources based on socio-economic and ecology development has led to a focus on the bioeconomy in the policy discourse of non-timber forest products (NTFPs). Honey is an important NTFP with high socio-economic value, and its production involves millions of Indonesians. This article reviews the current status of honey-producing bee management, cultivation and harvesting system, marketing and socio-economic values, and the industry’s environmental function in Indonesia. This research utilized a meta-narrative review method to collect data and information from Google Scholar, Scopus, Science Direct, ResearchGate, Sinta, and Garuda. The study showed that the four bee species, namely Apis mellifera, Apis cerana, Apis dorsata, and stingless bee, are the most common species in honey production in Indonesia. The four species have specific characteristics based on habitat, production capacity, derivative products, management intervention to meet honey product standards, and sustainable livelihoods. The value chain of bees’ major products, such as honey, propolis, pollen, royal jelly, wax, and other derivative products, involves the distribution of honey to all involved communities, including beekeepers, honey gatherers/hunters, intermediate traders, and the processing industry. This study also found a significant association between environmental sustainability statutes that affects functional sustainability and economic function. The finding parallels the global trends that put forward a forest-based bioeconomy approach to forest resource management. The policy must be strengthened in managing relationships among supporting actors for sustainable honey production.