bluuebunny/crossref_metadata_2025 huggingface dataset:
Preoperative Motor Function Associated with Short-Term Gain of Health-Related Quality of Life after Surgery for Lumbar Degenerative Disease: A Pilot Prospective Cohort Study in Japan
Authors: Ishibashi Yuya , Tomita Yosuke , Imura Shigeyuki , Takeuchi Nobuyuki
Published: December 2023

This study aimed to estimate the relationship between preoperative motor function and short-term recovery of health-related quality of life after lumbar surgery in patients with lumbar degenerative disease. This prospective cohort study involved 50 patients with lumbar degenerative disease at a general hospital in Japan. The primary outcome was the achievement of minimal clinically important difference (MCID) for EuroQOL 5 dimensions (EQ-5D) at discharge. Preoperative demographic, medication, surgical, and physical function data were collected. Logistic regression analysis was performed using the achievement of MCID for EQ-5D as the dependent variable and preoperative characteristics, including the Five Times Sit to Stand test (FTSTS), Oswestry Disability Index (ODI), and Self-rating Depression Scale (SDS), as the independent variables. The logistic regression analysis showed that Model 1 had a moderate predictive accuracy (Nagelkerke R2: 0.20; Hosmer–Lemeshow test: p = 0.19; predictive accuracy: 70.0%). Among the independent variables in the logistic regression model, the FTSTS was the only independent variable related to the achievement of MCID for EQ-5D at discharge (odds ratio: 0.03; 95% CI: 1.79 × 10−3, 0.18). Our results highlighted the importance of baseline motor function in the postoperative recovery of health-related quality of life in individuals with lumbar degenerative disease.
The Longitudinal Associations of Body Dissatisfaction with Health and Wellness Behaviors in Midlife and Older Women
Authors: Kilpela Lisa Smith , Hooper Savannah C. , Straud Casey L. , Marshall Victoria B. , Verzijl Christina L. , Stewart Tiffany M. , Loera Taylur T. , Becker Carolyn Black
Published: December 2023

Emerging research suggests that body dissatisfaction (BD) is prevalent among midlife and older women (i.e., upwards of 70%). Cross-sectionally, BD is associated with myriad poor health and wellness outcomes (e.g., depression, disordered eating, bad nutrition) in midlife/older women. However, relatively few studies have examined the longitudinal relations between BD and health outcomes in this population. This preliminary study investigated the longitudinal associations of BD with wellbeing and health-related quality of life (QOL) among midlife/older adult women over one year. Participants (n = 86, women aged 40–72 years, M = 51.49, SD = 7.34, 86% white) completed self-report measures of BD, psychosocial impairment, health behaviors, and QOL at baseline (T1) and 12-month follow-up (T2). A series of multiple linear regression models included T1 BD as the predictor variable of health outcomes at T2, covarying for T1 BMI and age in all models. BD was associated with greater negative emotions and psychosocial impairment, less physical activity enjoyment, and poorer physical, psychological, and social QOL one year later. Findings suggest that BD is associated with negative consequences for women across the lifespan (ƒ2 ranges = 0.06–0.60). Future research investigating BD as a unique, modifiable risk factor for health outcomes among diverse samples of midlife/older women is warranted. Targeting BD in interventions may improve health indices beyond eating disorders for this population.
Serotonin and Interleukin 10 Can Influence the Blood and Urine Viscosity in Gestational Diabetes Mellitus and Pregnancy-Specific Urinary Incontinence
Authors: França Danielle Cristina Honório , Honorio-França Adenilda Cristina , Silva Kênia Maria Rezende , Alves Fernanda Cristina Bérgamo , Bueno Gabriela , Costa Sarah Maria Barneze , Cotrim Aron Carlos de Melo , Barbosa Angélica Mércia Pascon , França Eduardo Luzía , Rudge Marilza Vieira Cunha , The Diamater Study Group
Published: December 2023

Serotonin and interleukin 10 (IL-10) may play a role in gestational diabetes mellitus. Hyperglycemic environment, the detrusor musculature of the bladder and pelvic floor muscles may become damaged, leading to urination problems and urine viscosity in pregnant women with gestational diabetes mellitus and pregnancy-specific urinary incontinence. Urine and blood samples were collected from pregnant women between 24 and 28 weeks of gestation. The serotonin concentration and cytokine IL-10 levels were evaluated in plasma and urine. In the total blood and urine, the viscosity was evaluated in the presence and absence of exogenous serotonin and IL-10. The plasma serotonin levels decreased, while the urine serotonin levels increased in the normoglycemic incontinent (NG-I), hyperglycemic continent (GDM-C), and hyperglycemic incontinent (GDM-I) groups. The IL-10 in the plasma decreased in the GDM-I group and was higher in the urine in the NG-I and GDM-I groups. The blood viscosity was higher, independently of urinary incontinence, in the GDM groups. The serotonin increased the blood viscosity from women with GDM-C and urine in the NG-I, GDM-C, and GDM-I groups. Blood and urine in the presence of IL-10 showed a similar viscosity in all groups studied. Also, no difference was observed in the viscosity in either the blood or urine when in the presence of serotonin and IL-10. These findings suggest that serotonin and IL-10 have the potential to reduce blood viscosity in pregnant women with gestational diabetes and specific urinary incontinence, maintaining values similar to those in normoglycemic women’s blood.
Effects of Anxious Depression on Antidepressant Treatment Response
Authors: Hampf Chantal , Scherf-Clavel Maike , Weiß Carolin , Klüpfel Catherina , Stonawski Saskia , Hommers Leif , Lichter Katharina , Erhardt-Lehmann Angelika , Unterecker Stefan , Domschke Katharina , Kittel-Schneider Sarah , Menke Andreas , Deckert Jürgen , Weber Heike
Published: December 2023

Anxious depression represents a subtype of major depressive disorder and is associated with increased suicidality, severity, chronicity and lower treatment response. Only a few studies have investigated the differences between anxious depressed (aMDD) and non-anxious depressed (naMDD) patients regarding treatment dosage, serum-concentration and drug-specific treatment response. In our naturalistic and prospective study, we investigated whether the effectiveness of therapy including antidepressants (SSRI, SNRI, NaSSA, tricyclics and combinations) in aMDD patients differs significantly from that in naMDD patients. In a sample of 346 patients, we calculated the anxiety somatization factor (ASF) and defined treatment response as a reduction (≥50%) in the Hamilton Depression Rating Scale (HDRS)-21 score after 7 weeks of pharmacological treatment. We did not observe an association between therapy response and the baseline ASF-scores, or differences in therapy outcomes between aMDD and naMDD patients. However, non-responders had higher ASF-scores, and at week 7 aMDD patients displayed a worse therapy outcome than naMDD patients. In subgroup analyses for different antidepressant drugs, venlafaxine-treated aMDD patients showed a significantly worse outcome at week 7. Future prospective, randomized-controlled studies should address the question of a worse therapy outcome in aMDD patients for different psychopharmaceuticals individually.
Extracellular Vesicles as Delivery Systems in Disease Therapy
Authors: Picon Manuel Alejandro , Wang Liyong , Da Fonseca Ferreira Andrea , Dong Chunming , Marzouka George R.
Published: December 2023

Extracellular vesicles (EVs)/exosomes are nanosized membrane-bound structures that are released by virtually all cells. EVs have attracted great attention in the scientific community since the discovery of their roles in cell-to-cell communication. EVs’ enclosed structure protects bioactive molecules from degradation in the extracellular space and targets specific tissues according to the topography of membrane proteins. Upon absorption by recipient cells, EV cargo can modify the transcription machinery and alter the cellular functions of these cells, playing a role in disease pathogenesis. EVs have been tested as the delivery system for the mRNA COVID-19 vaccine. Recently, different therapeutic strategies have been designed to use EVs as a delivery system for microRNAs and mRNA. In this review, we will focus on the exciting and various platforms related to using EVs as delivery vehicles, mainly in gene editing using CRISPR/Cas9, cancer therapy, drug delivery, and vaccines. We will also touch upon their roles in disease pathogenesis.
Decavanadate-Bearing Guanidine Derivatives Developed as Antimicrobial and Antitumor Species
Authors: Dumitrescu Andreea , Maxim Catalin , Badea Mihaela , Rostas Arpad Mihai , Ciorîță Alexandra , Tirsoaga Alina , Olar Rodica
Published: December 2023

To obtain biologically active species, a series of decavanadates (Hpbg)4[H2V10O28]·6H2O (1) (Htbg)4[H2V10O28]·6H2O; (2) (Hgnd)2(Hgnu)4[V10O28]; (3) (Hgnu)6[V10O28]·2H2O; and (4) (pbg = 1-phenyl biguanide, tbg = 1-(o-tolyl)biguanide, gnd = guanidine, and gnu = guanylurea) were synthesized and characterized by several spectroscopic techniques (IR, UV-Vis, and EPR) as well as by single crystal X-ray diffraction. Compound (1) crystallizes in space group P-1 while (3) and (4) adopt the same centrosymmetric space group P21/n. The unusual signal identified by EPR spectroscopy was assigned to a charge-transfer π(O)→d(V) process. Both stability in solution and reactivity towards reactive oxygen species (O2− and OH·) were screened through EPR signal modification. All compounds inhibited the development of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis bacterial strains in a planktonic state at a micromolar level, the most active being compound (3). However, the experiments conducted at a minimal inhibitory concentration (MIC) indicated that the compounds do not disrupt the biofilm produced by these bacterial strains. The cytotoxicity assayed against A375 human melanoma cells and BJ human fibroblasts by testing the viability, lactate dehydrogenase, and nitric oxide levels indicated compound (1) as the most active in tumor cells.
Importance of Metalloproteinase Enzyme Group in Selected Skeletal System Diseases
Authors: Kulesza Monika , Kicman Aleksandra , Motyka Joanna , Guszczyn Tomasz , Ławicki Sławomir
Published: December 2023

Bone tissue is a dynamic structure that is involved in maintaining the homeostasis of the body due to its multidirectional functions, such as its protective, endocrine, or immunological role. Specialized cells and the extracellular matrix (ECM) are responsible for the remodeling of specific bone structures, which alters the biomechanical properties of the tissue. Imbalances in bone-forming elements lead to the formation and progression of bone diseases. The most important family of enzymes responsible for bone ECM remodeling are matrix metalloproteinases (MMPs)—enzymes physiologically present in the body’s tissues and cells. The activity of MMPs is maintained in a state of balance; disruption of their activity is associated with the progression of many groups of diseases, including those of the skeletal system. This review summarizes the current understanding of the role of MMPs in bone physiology and the pathophysiology of bone tissue and describes their role in specific skeletal disorders. Additionally, this work collects data on the potential of MMPs as bio-markers for specific skeletal diseases.
Advances in Detection Techniques for the H5N1 Avian Influenza Virus
Authors: Fu Xianshu , Wang Qian , Ma Biao , Zhang Biao , Sun Kai , Yu Xiaoping , Ye Zihong , Zhang Mingzhou
Published: December 2023

Avian influenza is caused by avian influenza virus infection; the H5N1 avian influenza virus is a highly pathogenic subtype, affecting poultry and human health. Since the discovery of the highly pathogenic subtype of the H5N1 avian influenza virus, it has caused enormous losses to the poultry farming industry. It was recently found that the H5N1 avian influenza virus tends to spread among mammals. Therefore, early rapid detection methods are highly significant for effectively preventing the spread of H5N1. This paper discusses the detection technologies used in the detection of the H5N1 avian influenza virus, including serological detection technology, immunological detection technology, molecular biology detection technology, genetic detection technology, and biosensors. Comparisons of these detection technologies were analyzed, aiming to provide some recommendations for the detection of the H5N1 avian influenza virus.
The Potential Role of Nitric Oxide as a Therapeutic Agent against SARS-CoV-2 Infection
Authors: Xiao Shuqi , Yuan Zhiming , Huang Yi
Published: December 2023

The global coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become the greatest worldwide public health threat of this century, which may predispose multi-organ failure (especially the lung) and death despite numerous mild and moderate symptoms. Recent studies have unraveled the molecular and clinical characteristics of the infectivity, pathogenicity, and immune evasion of SARS-CoV-2 and thus improved the development of many different therapeutic strategies to combat COVID-19, including treatment and prevention. Previous studies have indicated that nitric oxide (NO) is an antimicrobial and anti-inflammatory molecule with key roles in pulmonary vascular function in the context of viral infections and other pulmonary disease states. This review summarized the recent advances of the pathogenesis of SARS-CoV-2, and accordingly elaborated on the potential application of NO in the management of patients with COVID-19 through antiviral activities and anti-inflammatory properties, which mitigate the propagation of this disease. Although there are some limits of NO in the treatment of COVID-19, it might be a worthy candidate in the multiple stages of COVID-19 prevention or therapy.
Prevalence of Sleep Disturbances in Latin American Populations and Its Association with Their Socioeconomic Status—A Systematic Review and a Meta-Analysis
Authors: Etindele Sosso F. A. , Torres Silva Filipa , Queiroz Rodrigues Rita , Carvalho Margarida M. , Zoukal Sofia , Zarate Gabriel Cordova
Published: December 2023

Background: The worldwide increase in the prevalence and incidence of sleep disturbances represents a major public health issue. Among multiple determinants affecting sleep health, an individual’s socioeconomic status (SES) is the most ignored and underestimated throughout the literature. No systematic review on the relation between SES and sleep health has been previously conducted in Latin America. Methods: PRISMA guidelines were used. Results: Twenty articles were included in the final sample (all cross-sectional studies), and twelve among them were rated as fair or poor quality. Among these studies, 80.0% (n = 16) were performed in Brazil, 10.0% (n = 2) were performed in Peru, 5.0% (n = 1) were performed in Chile, and 5.0% (n = 1) were multicentric (11 countries). The combined total number of participants was N = 128.455, comprising 3.7% (n = 4693) children, 16.0% (n = 20,586) adolescents, and 80.3% (n = 103,176) adults. The results show the following: (1) The sleep outcomes analyzed were sleep duration, sleep quality/sleep disturbance, insomnia, excessive daytime sleepiness (EDS), obstructive sleep apnea (OSA)/sleep-disordered breathing (SDB) symptoms, and bruxism. (2) The most used determinants were income, education level, employment status/occupation, wealth/assets, and composite indices. (3) Higher SES was associated with shorter sleep duration. (4) Lower SES was associated with a decrease in sleep quality, less frequent snoring, more prevalent EDS, and sleep bruxism. (5) Lower education was associated with insomnia. (6) Higher education was associated with more sleep bruxism. (7) The pooled prevalence using a meta-analysis of the random effects model was 24.73% (95%CI, 19.98–30.19), with high heterogeneity (I2 = 100%). (8) The prevalence of sleep disturbances decreased with high education (OR, 0.83; 95%CI, [0.69–0.99]; I2 = 79%), while it increased with low income (OR, 1.26; 95%CI, [1.12–1.42]; I2 = 59%), unemployment (OR, 2.84; 95%CI, [2.14–3.76]; I2 = 0%), and being a housewife (OR, 1.72; 95%CI, [1.19–2.48]; I2 = 55%). Discussion: This meta-analysis shows that lower SES (education, income, and work) was associated with sleep disturbances in Latin America. Therefore, sleep disturbance management should be addressed with a multidimensional approach, and a significant investment in targeted public health programs to reduce sleep disparities and support research should be made by the government before the situation becomes uncontrollable.
The Impact of Hematological Indices on the Occurrence of Delayed Graft Function (DGF) of Transplanted Kidney
Authors: Pilichowska Ewa , Ostrowski Piotr , Sieńko Jerzy
Published: December 2023

Background: to analyse the effect of haematological indices on the occurrence of Delayed Graft Function (DGF) in patients undergoing kidney transplantation and on the function of the transplanted kidney on the 7th postoperative day. Methods: 365 recipients who underwent kidney transplantation from a donor with known brain death between 2010 and 2017 were included in this retrospective study. Information from patient medical records, donor medical records, and donation and transplantation protocols was used for analysis. Statistica 13 was used for statistical analysis. Results: In the study group, DGF occurred in 144 recipients (39.45%), and Non-Graft Function (NGF) occurred in 12 recipients (3.29%). Recipients who developed DGF had a significantly higher Neutrophil/Monocyte Ratio (NMR) before renal transplantation (p = 0.048), a lower NMR value on postoperative day 1 (p < 0.001), and a difference between the values on day 1 and before surgery (p < 0.001). In addition, they had a significantly lower Lymphocyte/Monocyte Ratio (LMR) on postoperative day 1 LMR 1 (p < 0.001). It was shown that the value of the indices based on the ROC curve—NMR1 > 29.29, NMR1-0 > 22.71, and LMR1 > 1.74 (respectively: AUC = 0.624; 95% CI 0.566–0.682; and p < 0.001/AUC = 0.622; 95% CI 0.563–0.680; and p < 0.001/AUC = 0.610; 95% CI 0.550–0.670; and p < 0.001)—can be used to identify recipients with a significant probability of DGF. Conclusions: the NMR and LMR parameters on the first postoperative day and the difference between the NMR values on the first post-transplant day and the first pre-transplant day are predictive factors associated with the risk of DGF.
Risk Factors for Spinal Cord Injury during Endovascular Repair of Thoracoabdominal Aneurysm: Review of the Literature and Proposal of a Prognostic Score
Authors: Brisard Laurent , El Batti Salma , Borghese Ottavia , Maurel Blandine
Published: December 2023

Despite recent improvements, spinal cord ischemia remains the most feared and dramatic complication following extensive aortic repair. Although endovascular procedures are associated with a lower risk compared with open procedures, this risk is still significant and must be considered. A combined medical and surgical approach may help to optimize the tolerance of the spinal cord to ischemia. The aim of this review is to describe the underlying mechanism involved in spinal cord injury during extensive endovascular aortic repair, to describe the different techniques used to improve spinal cord tolerance to ischemia—including the prophylactic or curative use of spinal drainage—and to propose our algorithm for spinal cord protection and the rational use of spinal drainage.
Effects of Mooring Line with Different Materials on the Dynamic Response of Offshore Floating Wind Turbine
Authors: Lian Yushun , Zhong Fan , Zheng Jinhai , Chen Wenxing , Ma Gang , Wang Shan , Yim Solomon C.
Published: December 2023

The influence of mooring systems with lines of different material on the dynamic response of a floating wind turbine is studied using a 5 MW OC4-DeepCwind semi-submersible wind turbine as a representative prototype in this study. Two types of mooring systems were designed using the MoorDyn module in OpenFAST software (v3.1.0): one uses chains, and the other uses a hybrid mooring line composed of chains and high-strength polyethylene (HMPE) ropes. A wind turbine with two types of mooring systems was simulated using the OpenFAST software. The results show that the floating wind turbine moored with the hybrid lines exhibited a larger heave and pitch motion than that moored using chains alone. At the same time, the surge displacement was smaller than that of the wind turbine using chains alone. In terms of mooring line tension, the mean and amplitude values of the hybrid mooring system at the location examined were smaller than those of the chain mooring system. Thus, using HMPE ropes in the mooring system can significantly reduce line loads. In addition, the HMPE ropes used in the floating wind turbine mooring system did not affect the power generation of the wind turbine. This study provides promising support data and observations for applying high-strength polyethylene (HMPE) ropes in mooring systems for floating wind turbines.
The Role of Almond-Leaved Pear Pyrus spinosa Forssk. in Mediterranean Pasturelands Carbon Storage and Woodlands Restoration
Authors: Bueno Rafael Silveira , Badalamenti Emilio , Gristina Luciano , Novara Agata , La Mantia Tommaso
Published: December 2023

A large portion of the Mediterranean basin suffers from a lack of organic carbon in the soil and low woody cover percentages, resulting in a very high risk of desertification. In such conditions, knowing the effects on below and above ground carbon sequestration of pioneer woody species is of great importance, although barely assessed at the individual level. In this study, we first investigated whether almond-leaved pear (Pyrus spinosa) individuals influence soil organic carbon (SOC) concentration and stock in comparison with surrounding pasturelands inside a natural reserve in Sicily, Italy. Second, we evaluated inter individual variability on such storage, testing the effects of plant height, basal diameter, canopy cover and tree structure (single or multiple stems). Soils under pear presented, on average, a significantly higher SOC than pasturelands (3.86% and 3.16%, respectively) as well as a lower bulk density (1.09 and 1.28 g cm−3, respectively). Due to a lower soil compaction, SOC stocks (130.3 and 113.9 Mg ha−1, respectively) did not differ significantly. Below and aboveground biomass carbon accounted for a small fraction of carbon stock, while neither pear structure, age nor tree structure significantly influenced SOC concentration and SOC stock. Despite the need of further investigations, our results indicate that pear may represent an excellent tree species to improve carbon storage, both while triggering the restoration of Mediterranean woodlands or increasing biodiversity in pasturelands and agroforestry systems, that, indeed, can hold high SOC if well managed.
Analysis of Temporal and Spatial Dynamics of Ecosystem Services and Trade-Offs/Synergies during Urbanization in the Loess Plateau, China
Authors: Liu Jiamin , Wang Hao , Tang Butian , Hui Le , Zhang Weijuan , Zhang Liwei , Jiao Lei
Published: December 2023

As a typical ecological fragile zone and an area with a high intensity of human activities, the Loess Plateau (LP) of China has significantly altered its ecosystem and the corresponding services under the influence of urbanization processes. However, most existing studies focus on the spatial and temporal changes of ecosystem services (ESs) and their interrelationships under the influence of ecological restoration works in the LP, leaving limited research on the impacts of urbanization on ESs. Therefore, this study constructed a research framework for exploring the spatio-temporal dynamics and interactions of ESs under the influence of urbanization based on time series data from 2000 to 2020. The results showed that: (1) based on the comprehensive urbanization level (CUL), developed and developing areas accounted for 5.63% of the total area; (2) for the whole LP, all ESs except Habitat Quality (HQ) showed an increased trend. HQ showed a trade-off with the other services, while there was a clear synergy between the other three types of services; (3) in terms of processes of urbanization, Carbon Sequestration, Water Yield and HQ gradually decreased with increased levels of urbanization, and Soil Conservation increased the least in developing areas. The trade-off between HQ and the other three services decreased with increasing urbanization, while the synergy between the other three services strengthened as urbanization deepened. These findings suggest that urbanization significantly impacts ESs. It is necessary to implement appropriate measures (e.g., sponge city construction, urban green space, etc.) to address the impacts of urbanization on ESs.
Fused Filament Fabrication for Metallic Materials: A Brief Review
Authors: Costa Jose M. , Sequeiros Elsa W. , Vieira Manuel F.
Published: December 2023

Fused filament fabrication (FFF) is an extrusion-based additive manufacturing (AM) technology mostly used to produce thermoplastic parts. However, producing metallic or ceramic parts by FFF is also a sintered-based AM process. FFF for metallic parts can be divided into five steps: (1) raw material selection and feedstock mixture (including palletization), (2) filament production (extrusion), (3) production of AM components using the filament extrusion process, (4) debinding, and (5) sintering. These steps are interrelated, where the parameters interact with the others and have a key role in the integrity and quality of the final metallic parts. FFF can produce high-accuracy and complex metallic parts, potentially revolutionizing the manufacturing industry and taking AM components to a new level. In the FFF technology for metallic materials, material compatibility, production quality, and cost-effectiveness are the challenges to overcome to make it more competitive compared to other AM technologies, like the laser processes. This review provides a comprehensive overview of the recent developments in FFF for metallic materials, including the metals and binders used, the challenges faced, potential applications, and the impact of FFF on the manufacturing (prototyping and end parts), design freedom, customization, sustainability, supply chain, among others.
Impact of Aggregate Grain Size on ASR-Induced Expansion
Authors: Zapała-Sławeta Justyna
Published: December 2023

Alkali–silica reaction (ASR) is a sequence of complex chemical processes, resulting in the formation of alkali silica gels with high swelling ability. ASR leads to the expansion of concrete and the degradation of its microstructure. The susceptibility of aggregates to alkali reaction depends, among other factors, on the type and origin of the aggregate, the presence of reactive forms of silica, the mineral composition, and the geometric properties of the aggregate, such as shape and grain size. This study aimed to investigate the impact of the grain size of polymineral post-glacial gravel aggregate, originating from the northern regions of Poland, on its susceptibility to ASR. The expansion of mortars made from polymineral aggregate and the cracking of grains and cement matrix due to the occurring reactions were analyzed. Based on the conducted research, it was observed that the expansion of mortars depends on the grain size of the aggregate. It was demonstrated that the fraction of reactive aggregate generating the most significant elongation of mortars is in the range of 1.0–2.0 mm. The reaction of silica with alkalis continued until the depletion of reactive components in the aggregate. The relationship between the progress of corrosive processes and the grain size of the aggregate was evident in the form of different linear elongation increments of mortars over time. The expansion of mortars was caused by the swelling ASR gel, inducing stress in the grain and the surrounding cementitious paste.
Analog Memory and Synaptic Plasticity in an InGaZnO-Based Memristor by Modifying Intrinsic Oxygen Vacancies
Authors: Mahata Chandreswar , So Hyojin , Kim Soomin , Kim Sungjun , Cho Seongjae
Published: December 2023

This study focuses on InGaZnO-based synaptic devices fabricated using reactive radiofrequency sputtering deposition with highly uniform and reliable multilevel memory states. Electron trapping and trap generation behaviors were examined based on current compliance adjustments and constant voltage stressing on the ITO/InGaZnO/ITO memristor. Using O2 + N2 plasma treatment resulted in stable and consistent cycle-to-cycle memory switching with an average memory window of ~95.3. Multilevel resistance states ranging from 0.68 to 140.7 kΩ were achieved by controlling the VRESET within the range of −1.4 to −1.8 V. The modulation of synaptic weight for short-term plasticity was simulated by applying voltage pulses with increasing amplitudes after the formation of a weak conductive filament. To emulate several synaptic behaviors in InGaZnO-based memristors, variations in the pulse interval were used for paired-pulse facilitation and pulse frequency-dependent spike rate-dependent plasticity. Long-term potentiation and depression are also observed after strong conductive filaments form at higher current compliance in the switching layer. Hence, the ITO/InGaZnO/ITO memristor holds promise for high-performance synaptic device applications.
Maltitol-Derived Sacrificial Agent for Enhancing the Compatibility between PCE and Cement Paste
Authors: Wang Huan , Zhao Weixun , Wang Siqi , Wang Chao , Du Qifei , Yan Yan , Yang Xianke , Lv Sa , Hu Hongliang , Jin Yujie , Kong Lingwei , Wang Ping , Chi Yaodan , Yang Xiaotian
Published: December 2023

At present, it is known that when there is clay in concrete, polycarboxylates (PCE) will preferably adsorb in the clay, so that PCE cannot be fully combined with cement particles, which reduces the workability of the cement slurry. In this paper, a new type of maltitol–ammonium salt cationic (KN-lm) sacrificial agent (SA) has been successfully developed via a simple method, which makes PCE easier to bond with cement particles in the cement slurry containing clay. The effect of KN-lm on the fluidity of clay-containing cement paste is studied, and the experimental results show that KN-lm, as an efficient SA of cement slurry, makes PCE more compatible with clay-containing cement slurry, and increases the initial fluidity of cement slurry by about 19%. Further investigations of TOC, XRD, and zeta potential measurements reveal that a KN-lm ion is only preferably adsorbed into clay compared to PCE through electrostatic adsorption but without having any crystal structure change, thus resulting in good dispersion of cement particles. The addition of KN-lm plays an important role in hindering the hydration expansion of the clay by preferential electrostatic adsorption, which means PCE cannot easily insert into the interlayer of the clay.
Special Issue: “Functionalized Nanomaterials and Structures for Biomedical Applications”
Authors: Balaure Paul Cătălin
Published: December 2023

Nanoscale drug formulations are under wider and wider investigation due to their multiple unique advantages, such as stealth properties which avoid opsonization in the blood stream; specific ligand guided drug delivery to injured cells and tissues; on-demand release in sharp response to a series of endogenous and exogenous stimuli, allowing for predetermined programmed delivery to specific loci in the body and possible timing with circadian rhythms; increased cellular uptake due to their small dimensions and the possibility of intracellular delivery of the therapeutic cargo, especially when functionalized with cell penetrating agents; increased stability in biological fluids; augmented bioavailability; and tailored pharmacokinetics and pharmacodynamics [...]
A Novel Multi-Directional Partitioning Method for Support-Free 3D Printing of Inner Runner Structural Components
Authors: Wen Quan , Bin Yuanyuan , Xie Hualong , Yang Jianyu
Published: December 2023

Three-dimensional printing has great advantages in manufacturing parts with complex internal structures. At present, there are still significant challenges in the unsupported 3D printing of thick-walled parts with inner runners. In this paper, a partitioning method for support-free-fabricating workparts with a built-in inner runner structure is proposed and discussed in detail. With the method, partitioning planes are firstly created according to the direction changes of the inner runner, a “top to bottom” method is used for avoiding the interference of the generated planes, and thirdly the outer surface of the workpiece is considered for a second partition for the support-free purpose. A key algorithm for calculating partitioning planes for inner runner structures is also proposed and introduced in detail, including the iteration method, the calculation for intersectional profile mass centers, and the discussion of the convergence. Algorithm analysis is also performed with a simple model for assessing the influence from the defined parameters, including the proximity Φs and the increment coefficient σ, on the iteration results as well as on the iteration process. Also, an application test is carried out on a column model with a complex inner runner structure built-in. The result from all the tests indicates that the proposed algorithm is successful in partitioning inner runner structures for support-free fabrication.
Permeation Flux Prediction of Vacuum Membrane Distillation Using Hybrid Machine Learning Techniques
Authors: Ismael Bashar H. , Khaleel Faidhalrahman , Ibrahim Salah S. , Khaleel Samraa R. , AlOmar Mohamed Khalid , Masood Adil , Aljumaily Mustafa M. , Alsalhy Qusay F. , Mohd Razali Siti Fatin , Al-Juboori Raed A. , Hameed Mohammed Majeed , Alsarayreh Alanood A.
Published: December 2023

Vacuum membrane distillation (VMD) has attracted increasing interest for various applications besides seawater desalination. Experimental testing of membrane technologies such as VMD on a pilot or large scale can be laborious and costly. Machine learning techniques can be a valuable tool for predicting membrane performance on such scales. In this work, a novel hybrid model was developed based on incorporating a spotted hyena optimizer (SHO) with support vector machine (SVR) to predict the flux pressure in VMD. The SVR–SHO hybrid model was validated with experimental data and benchmarked against other machine learning tools such as artificial neural networks (ANNs), classical SVR, and multiple linear regression (MLR). The results show that the SVR–SHO predicted flux pressure with high accuracy with a correlation coefficient (R) of 0.94. However, other models showed a lower prediction accuracy than SVR–SHO with R-values ranging from 0.801 to 0.902. Global sensitivity analysis was applied to interpret the obtained result, revealing that feed temperature was the most influential operating parameter on flux, with a relative importance score of 52.71 compared to 17.69, 17.16, and 14.44 for feed flowrate, vacuum pressure intensity, and feed concentration, respectively.
Unveiling the Probiotic Potential of the Anaerobic Bacterium Cetobacterium sp. nov. C33 for Enhancing Nile Tilapia (Oreochromis niloticus) Cultures
Authors: Colorado Gómez Mario Andrés , Melo-Bolívar Javier Fernando , Ruíz Pardo Ruth Yolanda , Rodriguez Jorge Alberto , Villamil Luisa Marcela
Published: December 2023

The bacterium strain Cetobacterium sp. C33 was isolated from the intestinal microbial content of Nile tilapia (O. niloticus) under anaerobic conditions. Given that Cetobacterium species are recognized as primary constituents of the intestinal microbiota in cultured Nile tilapia by culture-independent techniques, the adaptability of the C33 strain to the host gastrointestinal conditions, its antibacterial activity against aquaculture bacterial and its antibiotic susceptibility were assessed. The genome of C33 was sequenced, assembled, annotated, and subjected to functional inference, particularly regarding pinpointed probiotic activities. Furthermore, phylogenomic comparative analyses were performed including closely reported strains/species relatives. Comparative genomics with closely related species disclosed that the isolate is not phylogenetically identical to other Cetobacterium species, displaying an approximately 5% sequence divergence from C. somerae and a 13% sequence divergence from Cetobacterium ceti. It can be distinguished from other species through physiological and biochemical criteria. Whole-genome annotation highlighted that Cetobacterium sp. nov. C33 possesses a set of genes that may contribute to antagonism against competing bacteria and has specific symbiotic adaptations in fish. Additional in vivo experiments should be carried out to verify favorable features, reinforcing its potential as a probiotic bacterium.
Motility in Periweissella Species: Genomic and Phenotypic Characterization and Update on Motility in Lactobacillaceae
Authors: Fanelli Francesca , Montemurro Marco , Chieffi Daniele , Cho Gyu-Sung , Low Hui-Zhi , Hille Frank , Franz Charles M. A. P. , Fusco Vincenzina
Published: December 2023

The genus Weissella and the recently described genus Periweissella, to which some previously named Weissella species have been reclassified as a result of a taxogenomic assessment, includes lactic acid bacteria species with high biotechnological and probiotic potential. Only one species, namely, Periweissella (P.) beninensis, whose type strain has been shown to possess probiotic features, has so far been described to be motile. However, the availability of numerous genome sequences of Weissella and Periweissella species prompted the possibility to screen for the presence of the genetic determinants encoding motility in Weissella and Periweissellas spp. other than P. beninensis. Herein, we performed a comprehensive genomic analysis to identify motility-related proteins in all Weissella and Periweissella species described so far, and extended the analysis to the recently sequenced Lactobacillaceae spp. Furthermore, we performed motility assays and transmission electron microscopy (TEM) on Periweissella type strains to confirm the genomic prediction. The homology-based analysis revealed genes coding for motility proteins only in the type strains of P. beninensis, P. fabalis, P. fabaria and P. ghanensis genomes. However, only the P. beninensis type strain was positive in the motility assay and displayed run-and-tumble behavior. Many peritrichous and long flagella on bacterial cells were visualized via TEM, as well. As for the Lactobacillaceae, in addition to the species previously described to harbor motility proteins, the genetic determinants of motility were also found in the genomes of the type strains of Lactobacillus rogosae and Ligilactobacillus salitolerans. This study, which is one of the first to analyze the genomes of Weissella, Periweissella and the recently sequenced Lactobacillaceae spp. for the presence of genes coding for motility proteins and which assesses the associated motility phenotypes, provides novel results that expand knowledge on these genera and are useful in the further characterization of lactic acid bacteria.
Associations of Plastic Bottle Exposure with Infant Growth, Fecal Microbiota, and Short-Chain Fatty Acids
Authors: Tilves Curtis , Zhao Heather Jianbo , Differding Moira K. , Zhang Mingyu , Liu Tiange , Hoyo Cathrine , Østbye Truls , Benjamin-Neelon Sara E. , Mueller Noel T.
Published: December 2023

Background/Objectives: Murine models show that plastics, via their chemical constituents (e.g., phthalates), influence microbiota, metabolism, and growth. However, research on plastics in humans is lacking. Here, we examine how the frequency of plastic bottle exposure is associated with fecal microbiota, short-chain fatty acids (SCFAs), and anthropometry in the first year of life. Subjects/Methods: In 442 infants from the prospective Nurture birth cohort, we examined the association of frequency of plastic bottle feeding at 3 months with anthropometric outcomes (skinfolds, length-for-age, and weight-for-length) at 12 months of age and growth trajectories between 3 and 12 months. Furthermore, in a subset of infants (n = 70) that contributed fecal samples at 3 months and 12 months of age, we examined plastic bottle frequency in relation to fecal microbiota composition and diversity (measured by 16S rRNA gene sequencing of V4 region), and fecal SCFA concentrations (quantified using gas chromatography mass spectrometry). Results: At 3 months, 67.6% of infants were plastic bottle fed at every feeding, 15.4% were exclusively breast milk fed, and 48.9% were exclusively formula fed. After adjustment for potential confounders, infants who were plastic bottle fed less than every feeding compared to those who were plastic bottle fed at every feeding at 3 months did not show differences in anthropometry over the first 12 months of life, save for lower length-for-age z-score at 12 months (adjusted β = −0.45, 95% CI: −0.76, −0.13). Infants who were plastic bottle fed less than every feeding versus every feeding had lower fecal microbiota alpha diversity at 3 months (mean difference for Shannon index: −0.59, 95% CI: −0.99, −0.20) and lower isovaleric acid concentration at 3 months (mean difference: −2.12 μmol/g, 95% CI: −3.64, −0.60), but these results were attenuated following adjustment for infant diet. Plastic bottle frequency was not strongly associated with microbiota diversity or SCFAs at 12 months after multivariable adjustment. Frequency of plastic bottle use was associated with differential abundance of some bacterial taxa, however, significance was not consistent between statistical approaches. Conclusions: Plastic bottle frequency at 3 months was not strongly associated with measures of adiposity or growth (save for length-for-age) over the first year of life, and while plastic bottle use was associated with some features of fecal microbiota and SCFAs in the first year, these findings were attenuated in multivariable models with infant diet. Future research is needed to assess health effects of exposure to other plastic-based products and objective measures of microplastics and plastic constituents like phthalates.
In Vitro Leishmanicidal Activity of Copaiba Oil and Kojic Acid Combination on the Protozoan Leishmania (Leishmania) amazonensis and Host Cell
Authors: Moraes Lienne Silveira de , Galué-Parra Adan Jesús , Hage Amanda Anastácia Pinto , Moura Hévila Aragão , Garcia Marcus Savio Araujo , Macêdo Caroline Gomes , Rodrigues Ana Paula Drummond , Guilhon Giselle Maria Skelding Pinheiro , Silva Edilene Oliveira da
Published: December 2023

(1) Background: Leishmaniasis refers to a group of anthropozoonotic diseases caused by Leishmania. The major chemotherapeutic agent used for its treatment is Glucantime®®, but the search continues for new compounds that are economically viable and act on the protozoan without causing damage to the host cell. As an alternative approach, this study used a combination of copaiba oil (CO) and kojic acid (KA) to determine their in vitro action on host cells, on the Leishmania (Leishmania) amazonensis protozoan and its interaction with macrophages. (2) Methods: In vitro culture, analysis of cytokine release and microscopy assays were performed. Statistical analysis was performed with ANOVA (GraphPad Prism). (3) Results: The combination did not induce cytotoxic effects on macrophages after treatment but promoted morphological changes in the protozoan, such as nuclear alterations (apoptotic characteristics), alterations in the cellular body and an increase in the number of electrodense structures and acidocalcisomes, observed mainly at the concentrations of CO20KA50 and CO30KA50 μg/mL. We observed reductions in the intracellular amastigote number and in the production of proinflammatory cytokines, such as IL-6 and TNF-α, after treatment with CO30KA at 50 µg/mL. (4) Conclusions: We report here, for the first time, that the combination of CO and KA may be a promising approach against Leishmania (Leishmania) amazonensis.
Determination of Volatile Organic Compounds and Endogenous Extracts and Study of Expression Patterns of TPS and BSMT in the Flowers of Seven Lilium Cultivars
Authors: Zhang Peng , Ma Xiaoou , Zhang Qian , Guo Ziyu , Hao Junyi , Zhang Zhixuan , Sun Ming , Liu Yan
Published: December 2023

Lily is one of the most important cut flowers in the world, with a rich floral fragrance. To further explore the fragrance emission mechanisms of lily cultivars, headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME-GC-MS) and organic solvent extraction–gas chromatography–mass spectrometry (OSE-GC-MS) were used to unveil the volatile organic compounds (VOCs) and endogenous extracts of seven lily cultivars. Furthermore, real-time quantitative PCR (qRT-PCR) was used to determine the expression levels of two key genes (TPS and BSMT) related to the biosynthesis of monoterpenoids and methyl benzoate. The results show that forty-five VOCs were detected in the petals of seven lily cultivars, and the main compounds were monoterpenoids and phenylpropanoids/benzenoids. Dichloromethane was the best solvent for extracting the endogenous extracts of Lilium ‘Viviana’ petals and eighteen endogenous extracts were detected using dichloromethane to extract the petals of seven lily cultivars. Each compound’s emission ratio (natural logarithm of the ratio of VOC content to endogenous extract content) was calculated, and linear regression analyses between emission ratios and boiling points were conducted. Significant linear negative correlations existed between the emission ratios and boiling points of compounds, and the regression equations’ coefficients of determination (R2) were all greater than 0.7. TPS was expressed highly in ‘Viviana’, ‘Pink News’, and ‘Palazzo’, and BSMT was expressed highly in ‘Pink News’ and ‘Palazzo’. Correlation analyses between the gene expression levels and the monoterpenoids and methyl benzoate contents found that the TPS expression levels have strong positive correlations with monoterpenoids content, while no correlations were found between the expression levels of BSMT and the contents of methyl benzoate. This study lays the foundation for research on the release patterns of VOCs in the flowers of Lilium, and the breeding of lilies for their floral fragrance.
Ag@AuNP-Functionalized Capillary-Based SERS Sensing Platform for Interference-Free Detection of Glucose in Urine Using SERS Tags with Built-In Nitrile Signal
Authors: Si Yanmei , Wang Hua , Yan Yehao , Li Bingwen , Ni Zeyun , Shi Hongrui
Published: December 2023

A Ag@AuNP-functionalized capillary-based surface-enhanced Raman scattering (SERS) sensing platform for the interference-free detection of glucose using SERS tags with a built-in nitrile signal has been proposed in this work. Capillary-based SERS capture substrates were prepared by connecting 4-mercaptophenylboronic acid (MBA) to the surface of the Ag@AuNP layer anchored on the inner wall of the capillaries. The SERS tags with a built-in interference-free signal could then be fixed onto the Ag@AuNP layer of the capillary-based capture substrate based on the distinguished feature of glucose, which can form a bidentate glucose–boronic complex. Thus, many “hot spots” were formed, which produced an improved SERS signal. The quantitative analysis of glucose levels was realized using the interference-free SERS intensity of nitrile at 2222 cm−1, with a detection limit of about 0.059 mM. Additionally, the capillary-based disposable SERS sensing platform was successfully employed to detect glucose in artificial urine, and the new strategy has great potential to be further applied in the diagnosis and control of diabetes.