bluuebunny/crossref_metadata_2025 huggingface dataset:
Progress and Prospects of Nanocellulose-Based Membranes for Desalination and Water Treatment
Authors: Saud Asif , Saleem Haleema , Zaidi Syed Javaid
Published: April 2022

Membrane-based desalination has proved to be the best solution for solving the water shortage issues globally. Membranes are extremely beneficial in the effective recovery of clean water from contaminated water sources, however, the durability as well as the separation efficiency of the membranes are restricted by the type of membrane materials/additives used in the preparation processes. Nanocellulose is one of the most promising green materials for nanocomposite preparation due to its biodegradability, renewability, abundance, easy modification, and exceptional mechanical properties. This nanocellulose has been used in membrane development for desalination application in the recent past. The study discusses the application of membranes based on different nanocellulose forms such as cellulose nanocrystals, cellulose nanofibrils, and bacterial nanocellulose for water desalination applications such as nanofiltration, reverse osmosis, pervaporation, forward osmosis, and membrane distillation. From the analysis of studies, it was confirmed that the nanocellulose-based membranes are effective in the desalination application. The chemical modification of nanocellulose can definitely improve the surface affinity as well as the reactivity of membranes for the efficient separation of specific contaminants/ions.
Correction: Seleznev et al. Ceramic Cutting Materials and Tools Suitable for Machining High-Temperature Nickel-Based Alloys: A Review. Metals 2021, 11, 1385
Authors: Seleznev Anton , Pinargote Nestor Washington Solis , Smirnov Anton
Published: April 2022

In the original article [...]
Modulation of Signal Regulatory Protein α (SIRPα) by Plasmodium Antigenic Extract: A Preliminary In Vitro Study on Peripheral Blood Mononuclear Cells
Authors: Martins Priscilla da Costa , de Souza Hugo Amorim dos Santos , Blanco Carolina Moreira , Santos-de-Oliveira Luana , Pratt-Riccio Lilian Rose , Daniel-Ribeiro Cláudio Tadeu , Totino Paulo Renato Rivas
Published: April 2022

Signal regulatory protein α (SIRPα) is an immunoreceptor expressed in myeloid innate immune cells that signals for inhibition of both phagocytosis and inflammatory response. Malaria parasites have evolutionarily selected multiple mechanisms that allow them to evade host immune defenses, including the modulation of cells belonging to innate immunity. Notwithstanding, little attention has been given to SIRPα in the context of immunosuppressive states induced by malaria. The present study attempted to investigate if malaria parasites are endowed with the capacity of modulating the expression of SIRPα on cells of innate immune system. Human peripheral blood mononuclear cells (PBMC) from healthy individuals were incubated in the presence of lipopolysaccharide (LPS) or crude extracts of P. falciparum or P. vivax and then, the expression of SIRPα was evaluated by flow cytometry. As expected, LPS showed an inhibitory effect on the expression of SIRPα in the population of monocytes, characterized by cell morphology in flow cytometry analysis, while Plasmodium extracts induced a significant positive modulation. Additional phenotyping of cells revealed that the modulatory potential of Plasmodium antigens on SIRPα expression was restricted to the population of monocytes (CD14+CD11c+), as no effect on myeloid dendritic cells (CD14−CD11c+) was observed. We hypothesize that malaria parasites explore inhibitory signaling of SIRPα to suppress antiparasitic immune responses contributing to the establishment of infection. Nevertheless, further studies are still required to better understand the role of SIRPα modulation in malaria immunity and pathogenesis.
Extract from Black Soybean Cultivar A63 Extract Ameliorates Atopic Dermatitis-like Skin Inflammation in an Oxazolone-Induced Murine Model
Authors: Dorjsembe Banzragch , Nho Chu Won , Choi Yongsoo , Kim Jin-Chul
Published: April 2022

Black soybean has been used in traditional medicine to treat inflammatory diseases, cancer, and diabetes and as a nutritional source since ancient times. We found that Korean black soybean cultivar A63 has more cyanidin-3-O-glucoside, (C3G), procyanidin B2 (PB2), and epicatechin (EPC) contents than other cultivars and has beneficial effects on cell viability and anti-oxidation. Given the higher concentration of anthocyanidins and their strong anti-oxidant activity, we predicted that A63 extract could relieve inflammatory disease symptoms, including those of atopic dermatitis (AD). Here, we evaluated the anti-AD activity of A63 extract in an oxazolone (OXA)-induced mouse model. A63 extract treatment significantly reduced epidermal thickness and inflammatory cell infiltration, downregulated the expression of AD gene markers, including Interleukin (IL)-4 and IL-5, and restored damaged skin barrier tissues. Furthermore, A63 extract influenced the activation of the signal transducer and activator of transcription (STAT) 3 and STAT6, extracellular regulatory kinase (ERK), and c-Jun N-terminal kinase (JNK) signaling pathways, which play a crucial role in the development of AD. Altogether, our results suggest that A63 can ameliorate AD-like skin inflammation by inhibiting inflammatory cytokine production and STAT3/6 and Mitogen-activated protein kinase (MAPK) signaling and restoring skin barrier function.
Characterization of Natural Anthocyanin Indicator Based on Cellulose Bio-Composite Film for Monitoring the Freshness of Chicken Tenderloin
Authors: Boonsiriwit Athip , Itkor Pontree , Sirieawphikul Chanutwat , Lee Youn Suk
Published: April 2022

Intelligent packaging with indicators that provide information about the quality of food products can inform the consumer regarding food safety and reduce food waste. A solid material for a pH-responsive indicator was developed from hydroxypropyl methylcellulose (HPMC) composited with microcrystalline cellulose (MCC). MCC at 5%, 10%, 20%, and 30% w/w was introduced into the HPMC matrix and the physical, barrier, thermal, and optical properties of the HPMC/MCC bio-composite (HMB) films were analyzed. At 5, 10, and 20% MCC, improved mechanical, transparency, and barrier properties were observed, where HMB with 20% of MCC (H20MB) showed the best performance. Therefore, H20MB was selected as the biodegradable solid material for fabricating Roselle anthocyanins (RA) pH sensing indicators. The performance of the RA-H20MB indicator was evaluated by monitoring its response to ammonia vapor and tracking freshness status of chicken tenderloin. The RA-H20MB showed a clear color change with respect to ammonia exposure and quality change of chicken tenderloin; the color changed from red to magenta, purple and green, respectively. These results indicated that RA-H20MB can be used as a biodegradable pH sensing indicator to determine food quality and freshness.
The Potential Antidepressant Action of Duloxetine Co-Administered with the TAAR1 Receptor Agonist SEP-363856 in Mice
Authors: Ren Xia , Xiong Jiaying , Liang Lingzhi , Chen Yin , Zhang Guisen
Published: April 2022

Here, we explored the possible interaction between duloxetine and SEP-363856 (SEP-856) in depression-related reactions. The results showed that oral administration of duloxetine showed powerful antidepressant-like effects in both the forced swimming test (FST) and the suspension tail test (TST). SEP-856 orally administered alone also exerted an antidepressant-like effect in FST and TST, especially at doses of 0.3, 1, and 10 mg/kg. In addition, duloxetine (15 mg/kg) and SEP-856 (15 mg/kg) both showed antidepressant-like effects in the sucrose preference test (SPT). Most importantly, in the above experiments, compared with duloxetine alone, the simultaneous use of duloxetine and SEP-856 caused a more significant antidepressant-like effect. It is worth noting that doses of drug combination in FST and TST did not change the motor activities of mice in the open-field test (OFT). Thus, duloxetine and SEP-856 seem to play a synergistic role in regulating depression-related behaviors and might be beneficial for refractory depression.
DFT Investigations of Aun Nano-Clusters Supported on TiO2 Nanotubes: Structures and Electronic Properties
Authors: Wang Ying , Zhou Ge
Published: April 2022

TiO2 nanotubes (TiO2NTs) are beneficial for photogenerated electron separation in photocatalysis. In order to improve the utilization rate of TiO2NTs in the visible light region, an effective method is to use Aun cluster deposition-modified TiO2NTs. It is of great significance to investigate the mechanism of Aun clusters supported on TiO2NTs to strengthen its visible-light response. In this work, the structures, electronic properties, Mulliken atomic charge, density of states, band structure, and deformation density of Aun (n = 1, 8, 13) clusters supported on TiO2NTs were investigated by DMOL3. Based on published research results, the most stable adsorption configurations of Aun (n = 1, 8, 13) clusters supported with TiO2NTs were obtained. The adsorption energy increased as the number of Au adatoms increased linearly. The Aun clusters supported on TiO2NTs carry a negative charge. The band gaps of the three most stable structures of each adsorption system decreased compared to TiO2NTs; the valence top and the conduction bottom of the Fermi level come mainly from the contribution of 5d and 6s-Au. The electronic properties of the 5d and 6s impurity orbitals cause valence widening and band gap narrowing.
Silk Fibroin-Based Biomaterials for Tissue Engineering Applications
Authors: Li Guangfei , Sun Shan
Published: April 2022

Tissue engineering (TE) involves the combination of cells with scaffolding materials and appropriate growth factors in order to regenerate or replace damaged and degenerated tissues and organs. The scaffold materials serve as templates for tissue formation and play a vital role in TE. Among scaffold materials, silk fibroin (SF), a naturally occurring protein, has attracted great attention in TE applications due to its excellent mechanical properties, biodegradability, biocompatibility, and bio-absorbability. SF is usually dissolved in an aqueous solution and can be easily reconstituted into different forms, including films, mats, hydrogels, and sponges, through various fabrication techniques, including spin coating, electrospinning, freeze drying, and supercritical CO2-assisted drying. Furthermore, to facilitate the fabrication of more complex SF-based scaffolds, high-precision techniques such as micro-patterning and bio-printing have been explored in recent years. These processes contribute to the diversity of surface area, mean pore size, porosity, and mechanical properties of different silk fibroin scaffolds and can be used in various TE applications to provide appropriate morphological and mechanical properties. This review introduces the physicochemical and mechanical properties of SF and looks into a range of SF-based scaffolds that have recently been developed. The typical applications of SF-based scaffolds for TE of bone, cartilage, teeth and mandible tissue, cartilage, skeletal muscle, and vascular tissue are highlighted and discussed followed by a discussion of issues to be addressed in future studies.
Delving into the Therapeutic Potential of Carica papaya Leaf against Thrombocytopenia
Authors: Munir Seemal , Liu Zhi-Wei , Tariq Tayyaba , Rabail Roshina , Kowalczewski Przemysław Łukasz , Lewandowicz Jacek , Blecharczyk Andrzej , Abid Muhammad , Inam-Ur-Raheem Muhammad , Aadil Rana Muhammad
Published: April 2022

Thrombocytopenia is a clinical manifestation that refers to the low platelet count, i.e., <150 × 103/μL, of blood, resulting in imbalanced hemostasis, which leads to several fatal complications. The causative factors vary greatly, but, as a consequence, they interfere with platelet production and promote destruction, leading to death. Carica papaya leaf has unique therapeutic and medicinal characteristics against thrombocytopenia, and this is supported by scientific studies. Secondary metabolites and minerals in the leaf, such as carpaine and quercetin, promote platelet production, inhibit platelet destruction, and maintain platelet membrane through gene expression activity and the ceasing of viral proteases, respectively. This review explores the scientific studies that support the role of papaya leaf in the form of juice, extract, or powder against thrombocytopenia through animal modeling and clinical trials. Phytochemical profiles of C. papaya leaf revealed the presence of flavonoids, alkaloids, phenols, cardiac glycosides, tannins, terpenes, and saponins, which impart therapeutic potential to the leaf. The therapeutic benefits of the leaf include immunomodulatory, antiviral, antidiabetic, anticancer, antimalarial, antiangiogenic, antibacterial, and antioxidant activities. Several conducted scientific research studies have proved the efficacy of C. papaya leaf against thrombocytopenia, expanding the implication of natural sources to eradicate numerous ailments.
Phenolic Profile and Antioxidant, Antibacterial, and Antiproliferative Activity of Juglans regia L. Male Flowers
Authors: Żurek Natalia , Pawłowska Agata , Pycia Karolina , Grabek-Lejko Dorota , Kapusta Ireneusz Tomasz
Published: April 2022

Juglans regia L., walnut, is a large, long-living tree, cultivated in temperate climates around the world. It is highly appreciated for its nutritional kernels and high-quality timber. Its barks, leaves, and husk are used as dyes and in folk medicine as herbal remedies for several diseases. From a biological and chemical standpoint, relatively little is known about the male flowers of the tree. Therefore, the aim of the study was to evaluate the phenolic profile as well as in vitro antioxidant, antimicrobial, and antiproliferative activity of male Juglans regia L. flowers. Phenolic content was determined by UPLC/PDA/MS/MS analyses; antioxidant activity was assessed by five different methods; antimicrobial activity was evaluated against the six most common pathogenic strains of Gram-positive and Gram-negative bacteria, and antiproliferative properties were assessed against six cell lines. Most of the analyses carried out in this study were performed for the first time for this raw material. J. regia flower extract was characterized by a strong ability to scavenge DPPH˙ free radicals, hydroxyl radicals, and chelating metal ions. Among the examined bacterial strains and neoplastic lines, the strongest antimicrobial activity was shown against S. aureus, L. monocytogenes, and B. cereus, and cytotoxic activity against breast cancer, glioblastoma, and astrocytoma cells. Male J. regia flowers have also been found to be a rich source of phenolic compounds. The content of polyphenols in the extract was 4369.73 mg/100 g d.w., and 24 compounds from the group of flavonoids, phenolic acids, and juglunosides were identified. Additionally, a strong correlation between the content of polyphenols and the antioxidant capacity and cytotoxic activity was observed. This is why the tested J. regia flowers are an excellent source of effective natural antioxidant, antibacterial, and chemopreventive compounds that have potential to be used in the pharmaceutical or food industries.
Losartan Alleviates the Side Effects and Maintains the Anticancer Activity of Axitinib
Authors: Fu Ying , Saxu Rengui , Ridwan Kadir Ahmad , Yao Jiaping , Chen Xiaoxuan , Xu Xueping , Zheng Weida , Yu Peng , Teng Yuou
Published: April 2022

Axitinib is one of the most potent inhibitors of the vascular endothelial growth factor (VEGF) receptor and shows strong antitumor activity toward various malignant tumors. However, its severe side effects affect the quality of life and prognosis of patients. Losartan, which functions as a typical angiotensin receptor blocker, controls the average arterial pressure of patients with essential hypertension and protects against hypertension-related secondary diseases, including proteinuria and cardiovascular injury. To explore the effects of losartan on side effects caused by axitinib and its antitumor activity, several animal experiments were conducted. This study first analyzed and explored the effect of losartan on the amelioration of side effects in Wistar rats caused by axitinib. The results showed that the systolic blood pressure of Wistar rats was significantly increased by about 30 mmHg in 7 days of axitinib treatment, while the combination of losartan significantly reduced the blood pressure rise caused by axitinib. The Miles experimental model and mouse xenograft tumor model were further used to evaluate the effect of losartan on the antitumor effect of axitinib. The result clearly demonstrated that losartan has no significant influence on axitinib-related low vascular permeability and antitumor activity. In summary, our results showed that the combination of axitinib and losartan significantly reduced the side effects and maintained the antitumor effects of axitinib. This study provides information for overcoming VEGF receptor inhibitor-related side effects.
Cationic Axial Ligand Effects on Sulfur-Substituted Subphthalocyanines
Authors: Ogura Yusaku , Nakano Masahiro , Maeda Hajime , Segi Masahito , Furuyama Taniyuki
Published: April 2022

Herein, we report the synthesis of sulfur-substituted boron(III) subphthalocyanines (SubPcs) with cationic axial ligands. Subphthalocyanines were synthesized by a condensation reaction using the corresponding phthalonitriles and boron trichloride as a template. An aminoalkyl group was introduced on the central boron atom; this process was followed by N-methylation to introduce a cationic axial ligand. The peripheral sulfur groups shifted the Q band of SubPcs to a longer wavelength. The cationic axial ligands increased the polarity and enhanced the hydrophilicity of SubPcs. The effect of axial ligands on absorption and fluorescence properties is generally small. However, a further red shift was observed by introducing cationic axial ligands into the sulfur-substituted SubPcs. This change is similar to that in sulfur-substituted silicon(IV) phthalocyanines. The unique effect of the cationic axial ligand was extensively investigated by theoretical calculations and electrochemistry. In particular, the precise oxidation potential was determined using ionization potential measurements. Thus, the results of the present study provide a novel strategy for developing functional dyes and pigments based on SubPcs.
Preparation, Characterization, and Mechanism of Antifreeze Peptides from Defatted Antarctic Krill (Euphausia superba) on Lactobacillus rhamnosus
Authors: Liu Yu , Yu Xuena , Zhu Yanling , Yang Wei , Zeng Yan , Hu Yi , Jiang Wei
Published: April 2022

Defatted Antarctic krill powder is the main by-product in the manufacturing of krill oil. Exploring a high value-added approach for utilizing this protein-rich material has received much attention in research and industry. Given this, the preparation and primary characterization of antifreeze peptides from defatted Antarctic krill (AKAPs) were carried out in this study. The cryoprotective effect of AKAPs on Lactobacillus rhamnosus ATCC7469 was also investigated. The results showed that Protamex was the optimum protease for AKAP preparation from defatted Antarctic krill. AKAPs were found to be rich in short peptides, with the MW ranging from 600 to 2000 Da (69.2%). An amino acid composition analysis showed that AKAPs were rich in glutamic acid (18.71%), aspartic acid (12.19%), leucine (7.87%), and lysine (7.61%). After freezing, the relative survival rate of Lactobacillus rhamnosus in the 1.0 mg/mL AKAP-treated group (96.83%) was significantly higher than in the saline group (24.12%) (p < 0.05). AKAPs also retarded the loss of acidifying activity of L. rhamnosus after freezing. AKAPs showed even better cryoprotective activity than three commercial cryoprotectants (sucrose, skim milk, and glycerol). In addition, AKAPs significantly alleviated the decrease in β-galactosidase and lactic dehydrogenase activities of L. rhamnosus (p < 0.05). Furthermore, AKAPs effectively protected the integrity of L. rhamnosus cell membranes from freezing damage and alleviated the leakage of intracellular substances. These findings demonstrate that AKAPs can be a potential cryoprotectant for preserving L. rhamnosus, providing a new way to use defatted Antarctic krill.
Marked Efficiency Improvement of FAPb0.7Sn0.3Br3 Perovskite Light-Emitting Diodes by Optimization of the Light-Emitting Layer and Hole-Transport Layer
Authors: Hu Lufeng , Ye Zhixiang , Wu Dan , Wang Zhaojin , Wang Weigao , Wang Kai , Cui Xiangqian , Wang Ning , An Hongyu , Li Bobo , Xiang Bingxi , Qiu Mingxia
Published: April 2022

Highly luminescent FAPb0.7Sn0.3Br3 nanocrystals with an average photoluminescence (PL) quantum yield of 92% were synthesized by the ligand-assisted reprecipitation method. The 41-nm-thick perovskite film with a smooth surface and strong PL intensity was proven to be a suitable luminescent layer for perovskite light-emitting diodes (PeLEDs). Electrical tests indicate that the double hole-transport layers (HTLs) played an important role in improving the electrical-to-optical conversion efficiency of PeLEDs due to their cascade-like level alignment. The PeLED based on poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(4,40-(N-(p-butylphenyl))-diphenylamine)] (TFB)/poly(9-vinylcarbazole) (PVK) double HTLs produced a high external quantum efficiency (EQE) of 9%, which was improved by approximately 10.9 and 5.14 times when compared with single HTL PVK or the TFB device, respectively. The enhancement of the hole transmission capacity by TFB/PVK double HTLs was confirmed by the hole-only device and was responsible for the dramatic EQE improvement.
Impact of Adsorption of Straight Chain Alcohol Molecules on the Optical Properties of Calcite (10.4) Surface
Authors: Mokkath Junais Habeeb
Published: April 2022

Calcium carbonate plays a central role in controlling the chemistry of the oceans, biomineralization and oil production, to name a few. In this work, using density functional theory with semiempirical dispersion corrections and simplified TD-DFT using Tamm–Dancoff approximation, we investigated the impact of the adsorption of straight chain alcohol (ethanol and pentanol) molecules on the optical properties of a calcite (10.4) surface. Our results show that ethanol and/or pentanol molecules form a well-ordered monolayer (through their hydroxyl group with carbon chains sticking away in a standing-up position) on the calcite (10.4) surface. Additionally, we found intriguing modulations in the photoabsorption spectra and circular dichroism spectra. In particular, the latter was a unique optical fingerprint for a molecule-adsorbed calcite (10.4) surface. Our findings provide useful insights into the structural and optical features of calcite-based systems at the atomic level.
SRC Kinase-Mediated Tyrosine Phosphorylation of TUBB3 Regulates Its Stability and Mitotic Spindle Dynamics in Prostate Cancer Cells
Authors: Alfano Alan , Xu Jin , Yang Xi , Deshmukh Dhanraj , Qiu Yun
Published: April 2022

Tubulin is an integral part of the cytoskeleton and plays a pivotal role in cellular signaling, maintenance, and division. β-tubulin is also the molecular target for taxane compounds such as docetaxel (DTX) and cabazitaxel (CTX), both first-line treatments for several solid cancers. Increased expression of Class III β-tubulin (TUBB3), a primarily neural isoform of β-tubulin, correlates with taxane resistance and poor prognosis. Although tyrosine kinase c-Src has been implicated to phosphorylate β-tubulins during both hematopoietic and neural differentiation, the mechanisms by which Src modulates tubulins functions are still poorly understood. Here, we report, for the first time, that TUBB3 is phosphorylated at Tyrosine 340 (Y340) by c-SRC in prostate cancer cells. We also showed that Y340 phosphorylation regulates TUBB3 protein stability and subcellular localization. Furthermore, we demonstrated that inhibition of SRC kinase activity compromises spindle stability in mitotic cells, at least partly due to the lack of TUBB3 Y340 phosphorylation. Given the importance of TUBB3 as a clinical biomarker of poor prognosis and drug resistance, characterization of TUBB3 posttranslational regulation could potentially serve as new biomarkers for disease recurrence and/or treatment failure.
A Care Ethical Engagement with John Locke on Toleration
Authors: Randall Thomas
Published: April 2022

Care theorists have yet to outline an account of how the concept of toleration should function in their normative framework. This lack of outline is a notable gap in the literature, particularly for demonstrating whether care ethics can appropriately address cases of moral disagreement within contemporary pluralistic societies; in other words, does care ethics have the conceptual resources to recognize the disapproval that is inherent in an act of toleration while simultaneously upholding the positive values of care without contradiction? By engaging care ethics with John Locke’s (1632–1704) influential corpus on toleration, I answer the above question by building the bases for a novel theory of toleration as care. Specifically, I argue that care theorists can home in on an oft-overlooked aspect of Locke’s later thought: that the possibility of a tolerant society is dependent on a societal ethos of trustworthiness and civility, to the point where Locke sets out positive ethical demands on both persons and the state to ensure this ethos can grow and be sustained. By leveraging and augmenting Locke’s thought within the care ethical framework, I clarify how care ethics can provide meaningful solutions to moral disagreement within contemporary pluralistic societies in ways preferable to the capability of a liberal state.
A New Method for Measuring Water Holdup of Oil-Water Two-Phase Flow in Horizontal Wells
Authors: Wang Yanjun , Han Jianlong , Hao Zhiqiang , Zhou Lijian , Wang Xinjie , Shao Mingwei
Published: April 2022

The production profile logging technology of Oil-water two-phase flow is an important support technology for efficient development of horizontal oil wells. Moreover, the water holdup of Oil-water two-phase flow is one of the important dynamic measurement parameters for production profile logging technology. Flow patterns in horizontal oil wells are more complex than those in vertical oil wells, making water holdup measurements more difficult. In China, horizontal oil wells are characterized by low liquid production, high water cut and layered development; hence, the concentrating flow logging scheme is adopted to reduce the effects of flow patterns on measurement. In this paper, we proposed a new method of measuring water holdup of Oil-water two-phase flow for the production profile logging in horizontal oil wells. Firstly, the structure of water holdup sensors and the measuring principle of water holdup were introduced. Secondly, the dynamic test research was carried out on the horizontal well simulation facility by using the test prototype of the water holdup measurement method of Oil-water two-phase flow. Additionally, the response characteristics of water holdup sensors at five inclined angles were emphatically discussed. Finally, the measurement error of water holdup was calculated and evaluated in detail. Experimental results show that the absolute values of measurement error of water holdup are less than 5% while the total flow rate ranged from 10 m3/d to 200 m3/d, and the water cut ranged from 30% to 90%, respectively.
Infrared Small Target Detection Using Regional Feature Difference of Patch Image
Authors: Zhang Guofeng , Ma Hongbing , Hamdulla Askar
Published: April 2022

Aiming at a thorny issue, that conventional small target detection algorithm using local contrast method is not sensitive for residual background clutter, robustness of algorithms is not strong. A Gaussian fusion algorithm using multi-scale regional patch structure difference and Regional Brightness Level Measurement is proposed. Firstly, Regional Energy Cosine (REC) is constructed to measure the structural discrepancy among a small target with neighboring cells. At the same time, Regional Brightness Level Measurement (RBLM) is constructed utilizing the brightness difference characteristics between small target and background areas. Then, a brand new Gaussian fusion algorithm is proposed for the generated saliency map in multi-scale space to characterize the overall heterogeneity in original infrared small target and local neighborhood. Finally, a self-adapting separation algorithm is adopted with the objective to obtain a small target from background interference. This method is able to utmostly restrain background interference and enhance the target. Extensive qualitative and quantitative testing results display that the desired algorithm has remarkable performance in strengthening target region and restraining background interference compared with current algorithms.
Inter-Patient Congestive Heart Failure Detection Using ECG-Convolution-Vision Transformer Network
Authors: Liu Taotao , Si Yujuan , Yang Weiyi , Huang Jiaqi , Yu Yongheng , Zhang Gengbo , Zhou Rongrong
Published: April 2022

An attack of congestive heart failure (CHF) can cause symptoms such as difficulty breathing, dizziness, or fatigue, which can be life-threatening in severe cases. An electrocardiogram (ECG) is a simple and economical method for diagnosing CHF. Due to the inherent complexity of ECGs and the subtle differences in the ECG waveform, misdiagnosis happens often. At present, the research on automatic CHF detection methods based on machine learning has become a research hotspot. However, the existing research focuses on an intra-patient experimental scheme and lacks the performance evaluation of working under noise, which cannot meet the application requirements. To solve the above issues, we propose a novel method to identify CHF using the ECG-Convolution-Vision Transformer Network (ECVT-Net). The algorithm combines the characteristics of a Convolutional Neural Network (CNN) and a Vision Transformer, which can automatically extract high-dimensional abstract features of ECGs with simple pre-processing. In this study, the model reached an accuracy of 98.88% for the inter-patient scheme. Furthermore, we added different degrees of noise to the original ECGs to verify the model’s noise robustness. The model’s performance in the above experiments proved that it could effectively identify CHF ECGs and can work under certain noise.
Total Design in the Design and Development Process of a Remotely Operated Vehicle (ROV) with Particular Consideration of Sensorization
Authors: Ramos Teresa , Córdoba Antonio , Luque Amalia , de las Heras Ana
Published: April 2022

This paper provides a methodological proposal for the design and development process of a remotely operated vehicle (ROV). The design core and product design specifications (PDS) of Pugh’s Total Design model are considered, with a focus on the early stages of the product design and development process. A modularization of the functional groups of an ROV is proposed, focusing attention on the sensor system. The main concepts regarding ROVs are presented, Pugh’s Total Design model is explained, justifying the application interest in technological projects, a methodological proposal adapted to ROV projects is provided, based on Pugh’s Total Design model, with special interest in the early stages of the new product development process (NPD), the suitability of applying our own model of industrial design engineering in an ROV system is analyzed, and the contribution of this study is evaluated, proposing future work and lines of research.
Influence of Integration Schemes and Maneuvers on the Initial Alignment and Calibration of AUVs: Observability and Degree of Observability Analyses
Authors: Frutuoso Adriano , Silva Felipe O. , de Barros Ettore A.
Published: April 2022

The use of autonomous underwater vehicles (AUV) has increased in a wide range of sectors, including the oil and gas industry, military, and marine research. The AUV capabilities to operate without a direct human operator and untethered to a support vessel are features that have aroused interest in the marine environment. The localization of AUV is significantly affected by the initial alignment and the calibration of the navigation sensors. In this sense, this paper proposes a thorough observability analysis applied to the latter problem. The observability analysis is carried out considering three types of sensor fusion integration and a set of maneuvers, and the results are validated through numerical simulations. As main contribution of this paper, it is shown how the addition of position errors in the observation vector can decouple some gyro and accelerometer biases from the latitude and altitude errors, particularly in the stationary observability analysis. The influence of oscillations in the diving plane and typical AUV maneuvers are analyzed, showing their relative impacts on the degree of observability of the inertial measurement unit (IMU)/Doppler velocity log (DVL) misalignment and DVL scale factor error. Finally, the state’s estimation accuracy is also analyzed, showing the limitation of the degree of observability as an assessment tool for the estimability of the states.
Condition Monitoring of the Dampers in the Railway Vehicle Suspension Based on the Vibrations Response Analysis of the Bogie
Authors: Dumitriu Mădălina
Published: April 2022

This paper investigates the possibility of developing a new method for fault detection of a damper in the primary suspension of the railway vehicle, based on the analysis of the vertical vibration’s response of the bogie. To this purpose, experimental data are used, along with results from numerical simulations regarding the Root Mean Square (RMS) accelerations measured/simulated in four reference bogie points—two points on the chassis, against the suspension, and two points located against the axle boxes. The experimental data are utilized to define the normal area of operating and the damper failure area in the bogie primary suspension, as well as a basis for validating the results of numerical simulations. The numerical simulations are developed on the basis of two original models of the vehicle–track system, rigid-flexible coupled type, which take into account the elasticity of the vehicle carbody and the elasticity of the wheel-rail contact: a reference model with 15 degrees of freedom, for simulating the bogie response to vertical vibrations for the normal operating of the primary suspension dampers, and an extended model with 20 degrees of freedom, for simulating the bogie vibration response to the failure damper of a primary suspension. The presented results show that there are clear premises on the possibilities of developing a fault detection method of any of the four dampers of the primary suspension corresponding to a vehicle bogie, based on the RMS accelerations measured only in two reference points of the bogie.
Active Opto-Magnetic Biosensing with Silicon Microring Resonators
Authors: Borga Piero , Milesi Francesca , Peserico Nicola , Groppi Chiara , Damin Francesco , Sola Laura , Piedimonte Paola , Fincato Antonio , Sampietro Marco , Chiari Marcella , Melloni Andrea , Bertacco Riccardo
Published: April 2022

Integrated optical biosensors are gaining increasing attention for their exploitation in lab-on-chip platforms. The standard detection method is based on the measurement of the shift of some optical quantity induced by the immobilization of target molecules at the surface of an integrated optical element upon biomolecular recognition. However, this requires the acquisition of said quantity over the whole hybridization process, which can take hours, during which any external perturbation (e.g., temperature and mechanical instability) can seriously affect the measurement and contribute to a sizeable percentage of invalid tests. Here, we present a different assay concept, named Opto-Magnetic biosensing, allowing us to optically measure off-line (i.e., post hybridization) tiny variations of the effective refractive index seen by microring resonators upon immobilization of magnetic nanoparticles labelling target molecules. Bound magnetic nanoparticles are driven in oscillation by an external AC magnetic field and the corresponding modulation of the microring transfer function, due to the effective refractive index dependence on the position of the particles above the ring, is recorded using a lock-in technique. For a model system of DNA biomolecular recognition we reached a lowest detected concentration on the order of 10 pm, and data analysis shows an expected effective refractive index variation limit of detection of 7.5×10−9 RIU, in a measurement time of just a few seconds.
Robot Navigation Based on Potential Field and Gradient Obtained by Bilinear Interpolation and a Grid-Based Search
Authors: Klančar Gregor , Zdešar Andrej , Krishnan Mohan
Published: April 2022

The original concept of the artificial potential field in robot path planning has spawned a variety of extensions to address its main weakness, namely the formation of local minima in which the robot may be trapped. In this paper, a smooth navigation function combining the Dijkstra-based discrete static potential field evaluation with bilinear interpolation is proposed. The necessary modifications of the bilinear interpolation method are developed to make it applicable to the path-planning application. The effect is that the strategy makes it possible to solve the problem of the local minima, to generate smooth paths with moderate computational complexity, and at the same time, to largely preserve the product of the computationally intensive static plan. To cope with detected changes in the environment, a simple planning strategy is applied, bypassing the static plan with the solution of the A* algorithm to cope with dynamic discoveries. Results from several test environments are presented to illustrate the advantages of the developed navigation model.
FOODCAM: A Novel Structured Light-Stereo Imaging System for Food Portion Size Estimation
Authors: Raju Viprav B. , Sazonov Edward
Published: April 2022

Imaging-based methods of food portion size estimation (FPSE) promise higher accuracies compared to traditional methods. Many FPSE methods require dimensional cues (fiducial markers, finger-references, object-references) in the scene of interest and/or manual human input (wireframes, virtual models). This paper proposes a novel passive, standalone, multispectral, motion-activated, structured light-supplemented, stereo camera for food intake monitoring (FOODCAM) and an associated methodology for FPSE that does not need a dimensional reference given a fixed setup. The proposed device integrated a switchable band (visible/infrared) stereo camera with a structured light emitter. The volume estimation methodology focused on the 3-D reconstruction of food items based on the stereo image pairs captured by the device. The FOODCAM device and the methodology were validated using five food models with complex shapes (banana, brownie, chickpeas, French fries, and popcorn). Results showed that the FOODCAM was able to estimate food portion sizes with an average accuracy of 94.4%, which suggests that the FOODCAM can potentially be used as an instrument in diet and eating behavior studies.
Purification of Andrographolide Methanolic Extract Using Molecularly Imprinted Polymer Prepared by Precipitation Polymerization
Authors: Winingsih Wiwin , Ibrahim Slamet , Damayanti Sophi
Published: April 2022

Molecularly Imprinted Polymer (MIP) has a specific cavity in which the conformity of shape, size, and functionalities corresponds with its template molecule and has been widely used in separation processes. Therefore, this study aims to examine the application of MIP for the purification of andrographolide. The MIP was synthesized by precipitation polymerization using methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) as the functional monomer and cross-linker, andrographolide as a template, and acetonitrile:toluene (3:1) as porogen solvent. The results showed that the binding capacity of Synthesized MIP was 1.2486 mg/g, while the particle size was 295.5 nm with a polydispersity index of 0.064. Furthermore, the imprinting and selectivity factors were 1.148 and 12.37, respectively. The purification process by MIP increased the purity from 55.37 ± 0.69 to 94.94% ± 0.34, while the isolate characterization showed that purified andrographolide had a similar character compared to the standard.
Separation Abilities of Capillary Electrophoresis Coupled with Ion Mobility Mass Spectrometry for the Discrete Detection of Sequence Isomeric Peptides
Authors: Glazyrin Yury E. , Mironov Gleb G. , Kichkailo Anna S. , Berezovski Maxim V.
Published: April 2022

The separation and discrete detection of isomeric sequence peptides with similar properties are important tasks for analytical science. Three different peptide isomers of 12 amino-acid residues long, containing direct and reverse regions of the alanine-valine-proline-isoleucine (AVPI) motif, were partially separated and discretely detected from their mixture using two approaches. Capillary electrophoresis enabled the separation and optical detection of the peptide sequence isomers close to the baseline. The ability to separate these sequence isomers from the mixture and discretely identify them from mass spectra has also been demonstrated by ion-mobility tandem mass spectrometry. Moreover, for the first time, capillary electrophoresis and ion-mobility mass spectrometry connected online have shown their ability for a discrete detection of the multidirectional sequence isomers.
Amino-Functionalized Polystyrene Nano-Plastics Induce Mitochondria Damage in Human Umbilical Vein Endothelial Cells
Authors: Fu Yiqi , Fan Mengqi , Xu Liwang , Wang Hui , Hu Qinglian , Jin Yuanxiang
Published: April 2022

As emerging contaminants, nano-plastics have become a major cause for concern for their adverse effects on the ecosystem and human health. The nano-sized properties of nano-plastics enable their exposure risks to humans through the food chain or other ways. However, the fate and adverse impact of nano-plastics on the human cardiovascular system are lacking. In this regard, the human umbilical vein endothelial cell line HUVEC was applied as a cell model to investigate the biological effects of noncharged polystyrene nano-plastics (PS NPs) and amino-functionalized nano-plastics (NH2-PS NPs). The positively charged PS NPs exhibited higher cytotoxicity to HUVEC, as evidenced by the decreased cell viability, enhanced ROS generation, and decreased mitochondria membrane potential triggered by NH2-PS NPs. Importantly, RT-PCR analysis revealed that NH2-PS NPs dysregulated the mitochondrial dynamics, replication, and function-related gene expression. This study demonstrated that NH2-PS NPs presented higher risks to endothelial cells than non-charged nano-plastics by interfering with mitochondria, which supported the direct evidence and expanded the potential risks of PS NPs.
The 3D Direct Simulation Monte Carlo Study of Europa’s Gas Plume
Authors: Tseng Wei-Ling , Lai Ian-Lin , Ip Wing-Huen , Hsu Hsiang-Wen , Wu Jong-Shinn
Published: April 2022

Europa has been spotted as having water outgassing activities by space- and ground-based telescopes as well as reanalysis of the Galileo data. We adopt a 3D Direct Simulation Monte Carlo (DSMC) model to investigate the observed plume characteristics of Europa assuming that supersonic expansion originated from the subsurface vent. With a parametric study of the total gas production rate and initial gas bulk velocity, the gas number density, temperature and velocity information of the outgassing plumes from various case studies were derived. Our results show that the plume gases experience acceleration through mutual collisions and adiabatic cooling when exiting from the surface. The central part of the plume with relatively large gas production rates (1029 and 1030 H2O s−1) was found to sustain thermal equilibrium and near continuum condition. Column density maps integrated along two different viewing angles are presented to demonstrate the importance of the projection effect on remote sensing diagnostics. Finally, the density profiles at different altitudes are provided to prepare for observations of Europa’s plumes including upcoming spacecraft missions such as JUICE and Europa Clipper.
Epidemiological Investigation and Genetic Analysis of Pseudorabies Virus in Yunnan Province of China from 2017 to 2021
Authors: Yao Jun , Li Juan , Gao Lin , He Yuwen , Xie Jiarui , Zhu Pei , Zhang Ying , Zhang Xue , Duan Luoyan , Yang Shibiao , Song Chunlian , Shu Xianghua
Published: April 2022

In recent years, the prevalence of pseudorabies virus (PRV) has caused huge economic losses to the Chinese pig industry. Meanwhile, PRV infection in humans also sounded the alarm about its cross-species transmission from pigs to humans. To study the regional PRV epidemic, serological and epidemiological investigations of PRV in pig populations from Yunnan Province during 2017–2021 were performed. The results showed that 31.37% (6324/20,158, 95% CI 30.73–32.01) of serum samples were positive for PRV glycoprotein E (gE)-specific antibodies via enzyme-linked immunosorbent assay (ELISA). The risk factors, including the breeding scale and development stage, were significantly associated with PRV seroprevalence among pigs in Yunnan Province. Of the 416 tissue samples collected from PRV-suspected pigs in Yunnan Province, 43 (10.33%, 95% CI 7.41–13.26) samples were positive for PRV-gE nucleic acid in which 15 novel PRV strains from these PRV-positive samples were isolated, whose gC and gE sequences were analyzed. Phylogenetic analysis showed that all 15 isolates obtained in this study belonged to the genotype II. Additionally, the gC gene of one isolate (YuN-YL-2017) was genetically closer to variant PRV strains compared with others, while the gE gene was in the same clade with other classical PRV strains, indicating that this isolate might be a recombinant strain generated from the classical and variant strains. The results revealed the severe PRV epidemic in Yunnan Province and indicated that PRV variants are the major genotypes threatening the pig industry development.
Antimicrobial Resistance in Companion Animals: A New Challenge for the One Health Approach in the European Union
Authors: Marco-Fuertes Ana , Marin Clara , Lorenzo-Rebenaque Laura , Vega Santiago , Montoro-Dasi Laura
Published: April 2022

Antimicrobial resistance (AMR) and the increase in multi-resistant bacteria are among the most important threats to public health worldwide, according to the World Health Organisation (WHO). Moreover, this issue is underpinned by the One Health perspective, due to the ability of AMR to be transmitted between animals and humans living in the same environment. Therefore, since 2014 different surveillance and control programmes have been established to control AMR in commensal and zoonotic bacteria in production animals. However, public health authorities’ reports on AMR leave out companion animals, due to the lack of national programmes and data collection by countries. This missing information constitutes a serious public health concern due to the close contact between companion animals, humans and their surrounding environment. This absence of control and harmonisation between programmes in European countries leads to the ineffectiveness of antibiotics against common diseases. Thus, there is a pressing need to establish adequate surveillance and monitoring programmes for AMR in companion animals and further develop alternatives to antibiotic use in this sector, considering the impact this could have on the gut microbiota. In this context, the aim of this review is to evaluate the current control and epidemiological situations of AMR in companion animals in the European Union (EU), as well as the proposed alternatives to antibiotics.