bluuebunny/crossref_metadata_2025 huggingface dataset:
A Putative P-Type ATPase Regulates the Secretion of Hydrolytic Enzymes, Phospholipid Transport, Morphogenesis, and Pathogenesis in Phytophthora capsici
Authors: Yang Chengdong , Zheng Bowen , Wang Rongbo , Chang Hongyang , Liu Peiqing , Li Benjin , Norvienyeku Justice , Chen Qinghe
Published: May 2022

Phytophthora capsici is an important plant pathogenic oomycete with multiple hosts. The P4-ATPases, aminophospholipid translocases (APTs), play essential roles in the growth and pathogenesis of fungal pathogens. However, the function of P4-ATPase in P. capsici remains unclear. This study identified and characterized PcApt1, a P4-ATPase Drs2 homolog, in P. capsici. Deletion of PcAPT1 by CRISPR/Cas9 knock-out strategy impaired hyphal growth, extracellular laccase activity. Cytological analyses have shown that PcApt1 participates in phosphatidylserine (PS) transport across the plasma membrane. Also, we showed that targeted deletion of PcAPT1 triggered a significant reduction in the virulence of P. capsici. Secretome analyses have demonstrated that secretion of hydrolytic enzymes decreased considerably in the PcAPT1 gene deletion strains compared to the wild-type. Overall, our results showed that PcApt1 plays a pivotal role in promoting morphological development, phospholipid transport, secretion of hydrolytic enzymes, and the pathogenicity of the polycyclic phytopathogenic oomycete P. capsici. This study underscores the need for comprehensive evaluation of subsequent members of the P-type ATPase family to provide enhanced insights into the dynamic contributions to the pathogenesis of P. capsici and their possible deployment in the formulation of effective control strategies.
Carrageenan From Kappaphycus alvarezii (Rhodophyta, Solieriaceae): Metabolism, Structure, Production, and Application
Authors: Rupert Rennielyn , Rodrigues Kenneth Francis , Thien Vun Yee , Yong Wilson Thau Lym
Published: May 2022

Carrageenan is a polysaccharide derived from red algae (seaweed) with enormous economic potential in a wide range of industries, including pharmaceuticals, food, cosmetics, printing, and textiles. Carrageenan is primarily produced through aquaculture-based seaweed farming, withEucheumaandKappaphycusspecies accounting for more than 90% of global output. There are three major types of carrageenan found in red algae: kappa (κ)-, iota (ι)-, and lambda (λ)-carrageenan.Kappaphycus alvareziiis the most common kappa-carrageenan source, and it is primarily farmed in Asian countries such as Indonesia, the Philippines, Vietnam, and Malaysia. Carrageenan extracted fromK. alvareziihas recently received a lot of attention due to its economic potential in a wide range of applications. This review will discussK. alvareziicarrageenan in terms of metabolic and physicochemical structure, extraction methods and factors affecting production yield, as well as current and future applications.
Development of Whole Genome SNP-CAPS Markers and Preliminary QTL Mapping of Fruit Pedicel Traits in Watermelon
Authors: Amanullah Sikandar , Osae Benjamin Agyei , Yang Tiantian , Li Shenglong , Abbas Farhat , Liu Shi , Liu Shusen , Song Zhengfeng , Wang Xuezheng , Gao Peng , Luan Feishi
Published: May 2022

Fruit pedicel (FP) is an important determinant of premium fruit quality that directly affects commercial market value. However, in-depth molecular and genetic basis of pedicel-related traits has not been identified in watermelon. Herein, a quantitative trait locus (QTL) mapping strategy was used to identify the potential genetic regions controlling FP traits based on newly derived whole-genome single nucleotide polymorphism based cleaved amplified polymorphism sequence (SNP-CAPS) markers. Next-generation sequencing based whole-genome re-sequencing of two watermelon parent lines revealed 98.30 and 98.40% of average coverage, 4,989,869 SNP variants, and 182,949 CAPS loci pairs across the reference genome, respectively. A total of 221 sets of codominant markers exhibited 46.42% polymorphism rate and were effectively genotyped within 100-F2:3 derived mapping population. The developed linkage map covered a total of 2,630.49 cM genetic length with averaged 11.90 cM, and depicted a valid marker-trait association. In total, 6 QTLs (qFPL4.1, qFPW4.1, qFPD2.1, qFPD2.2, qFPD8.1, qFPD10.1) were mapped with five major effects and one minor effect between the whole genome adjacent markers positioned over distinct chromosomes (02, 04, 08, 10), based on the ICIM-ADD mapping approach. These significant QTLs were similarly mapped in delimited flanking regions of 675.10, 751.38, 859.24, 948.39, and 947.51 kb, which collectively explained 8.64–13.60% PVE, respectively. A highly significant and positive correlation was found among the observed variables. To our knowledge, we first time reported the mapped QTLs/genes affecting FP traits of watermelon, and our illustrated outcomes will deliver the potential insights for fine genetic mapping as well as functional gene analysis through MAS-based breeding approaches.
Spatial Difference of Interactive Effect Between Temperature and Daylength on Ginkgo Budburst
Authors: Wu Zhaofei , Wang Shuxin , Fu Yongshuo H. , Gong Yufeng , Lin Chen-Feng , Zhao Yun-Peng , Prevéy Janet S. , Zohner Constantin
Published: May 2022

Climate warming-induced shifts in spring phenology have substantially affected the structure and function of terrestrial ecosystems and global biogeochemical cycles. Spring phenology is primarily triggered by spring temperature and is also affected by daylength and winter chilling, yet the relative importance of these cues across spatial gradients remains poorly understood. Here, we conducted a manipulative experiment with two daylength and three temperature treatments to investigate spatial differences in the response of ginkgo budburst to temperature and daylength, using twigs collected at three sites across a spatial gradient: a control site at a low latitude and low elevation on Tianmu Mountain (TMlow), a low latitude and high elevation site on Tianmu Mountain (TMhigh), and a high latitude site on Jiufeng mountain (JF). The mechanisms were also tested using in situ phenological observations of ginkgo along latitudes in China. We found that, compared to TMlow individuals, budburst dates occurred 12.6 (JF) and 7.7 (TMhigh) days earlier in high-latitude and high-elevation individuals when exposed to the same temperature and daylength treatments. Importantly, daylength only affected budburst at low latitudes, with long days (16 h) advancing budburst in low-latitude individuals by, on average, 8.1 days relative to short-day (8 h) conditions. This advance was most pronounced in low-elevation/latitude individuals (TMlow = 9.6 days; TMhigh = 6.7 days; JF = 1.6 days). In addition, we found that the temperature sensitivity of budburst decreased from 3.4 to 2.4 days °C−1 along latitude and from 3.4 to 2.5 days °C−1 along elevation, respectively. The field phenological observations verified the experimental results. Our findings provide empirical evidence of spatial differences in the relative effects of spring temperature and daylength on ginkgo budburst, which improved our understanding of spatial difference in phenological changes and the responses of terrestrial ecosystem to climate change.
Combined Drought and Heat Stress Influences the Root Water Relation and Determine the Dry Root Rot Disease Development Under Field Conditions: A Study Using Contrasting Chickpea Genotypes
Authors: Chilakala Aswin Reddy , Mali Komal Vitthalrao , Irulappan Vadivelmurugan , Patil Basavanagouda S. , Pandey Prachi , Rangappa Krishnappa , Ramegowda Venkategowda , Kumar M. Nagaraj , Puli Chandra Obul Reddy , Mohan-Raju Basavaiah , Senthil-Kumar Muthappa
Published: May 2022

Abiotic stressors such as drought and heat predispose chickpea plants to pathogens of key importance leading to significant crop loss under field conditions. In this study, we have investigated the influence of drought and high temperature on the incidence and severity of dry root rot disease (caused by Macrophomina phaseolina) in chickpea, under extensive on- and off-season field trials and greenhouse conditions. We explored the association between drought tolerance and dry root rot resistance in two chickpea genotypes, ICC 4958 and JG 62, with contrasting resistance to dry root rot. In addition, we extensively analyzed various patho-morphological and root architecture traits altered by combined stresses under field and greenhouse conditions in these genotypes. We further observed the role of edaphic factors in dry root rot incidence under field conditions. Altogether, our results suggest a strong negative correlation between the plant water relations and dry root rot severity in chickpeas, indicating an association between drought tolerance and dry root rot resistance. Additionally, the significant role of heat stress in altering the dynamics of dry root rot and the importance of combinatorial screening of chickpea germplasm for dry root rot resistance, drought, and heat stress have been revealed.
Understanding and Exploiting Transpiration Response to Vapor Pressure Deficit for Water Limited Environments
Authors: Broughton Katrina J. , Conaty Warren C.
Published: May 2022

More frequent droughts and an increased pressure on water resources, combined with social licence to operate, will inevitably decrease water resources available for fully irrigated cotton production. Therefore, the long-term future of the cotton industry will require more drought tolerant varieties that can perform well when grown in rainfed cropping regions often exposed to intermittent drought. A trait that limits transpiration (TRLim) under an increased vapour pressure deficit (VPD) may increase crop yield in drier atmospheric conditions and potentially conserve soil water to support crop growth later in the growing season. However, this trait has not been tested or identified in cotton production systems. This study tested the hypotheses that (1) genetic variability to the TRLim VPD trait exists amongst 10 genotypes in the Australian cotton breeding programme; (2) genotypes with a TRLim VPD trait use less water in high VPD environments and (3) variation in yield responses of cotton genotypes is linked with the VPD environment and water availability during the peak flowering period. This study combined glasshouse and field experiments to assess plant transpiration and crop yield responses of predominantly locally bred cotton genotypes to a range of atmospheric VPD under Australian climatic conditions. Results indicated that genetic variation to the limiting transpiration VPD trait exists within cotton genotypes in the Australian breeding programme, with five genotypes identified as expressing the TRLim VPD trait. A modelling study suggests that this trait may not necessarily result in overall reduced plant water use due to greater transpiration rates at lower VPD environments negating the water conservation in high VPD environments. However, our study showed that the yield response of cotton genotypes is linked with both VPD environment and water availability during the peak flowering period. Yield performance of the TRLim genotype was improved at some high VPD environments but is unlikely to out-perform a genotype with a lower yield potential. Improved understanding of integrated plant- and crop-level genotypic responses to the VPD environments will enhance germplasm development to benefit cotton production in both rainfed and semi-irrigated cotton systems, thereby meeting the agricultural challenges of the twenty-first Century.
Hololectin Interdomain Linker Determines Asparaginyl Endopeptidase-Mediated Maturation of Antifungal Hevein-Like Peptides in Oats
Authors: Loo Shining , Tay Stephanie V. , Kam Antony , Lee Warren , Tam James P.
Published: May 2022

Heveins and hevein-containing (hev-) lectins play important roles in stress and pathogenic responses in plants but cause health concerns in humans. Hev-hololectins contain multiple modular hev-peptide domains and are abundantly present in cereals and pseudocereals. However, it is unclear why some cereal hev-hololectins are presented as different forms of proteolytically processed proteoforms. Here we show the precursor architectures of hev-hololectins lead to different processing mechanisms to give either hololectins or hevein-like peptides. We used mass spectrometry and datamining to screen hev-peptides from common cereals, and identified from the oat plant Avena sativa nine novel hevein-like peptides, avenatide aV1–aV9. Bioinformatic analysis revealed that asparaginyl endopeptidase (AEP) can be responsible for the maturation of the highly homologous avenatides from five oat hev-hololectin precursors, each containing four tandemly repeating, hev-like avenatide domains connected by AEP-susceptible linkers with 13–16 residues in length. Further analysis of cereal hev-hololectins showed that the linker lengths provide a distinguishing feature between their cleavable and non-cleavable precursors, with the cleavables having considerably longer linkers (>13 amino acids) than the non-cleavables (<6 amino acids). A detailed study of avenatide aV1 revealed that it contains eight cysteine residues which form a structurally compact, metabolic-resistant cystine-knotted framework with a well-defined chitin-binding site. Antimicrobial assays showed that avenatide aV1 is anti-fungal and inhibits the growth of phyto-pathogenic fungi. Together, our findings of cleavable and non-cleavable hololectins found in cereals expand our knowledge to their biosynthesis and provide insights for hololectin-related health concerns in human.
The Language of Extremism on Social Media: An Examination of Posts, Comments, and Themes on Reddit
Authors: Hiaeshutter-Rice Dan , Hawkins Ian
Published: May 2022

Digital media give the public a voice to discuss or share their thoughts about political and social events. However, these discussions can often include language that contributes to creating toxic or uncivil online environments. Using data from Reddit, we examine the language surrounding three major events in the United States that occurred in 2020 and early 2021 from the comments and posts of 65 communities identified for their focus on extreme content. Our results suggest that social and political events in the U.S. triggered increased hostility in discussions as well as the formation of a set of shared language for describing and articulating information about these major political/social moments. Findings further reveal shifts in language toward more negativity, incivility, and specific language surrounding non-White outgroups. Finally, these shifts in language online were found to be durable and last after the events occurred. Our project identifies that negative language is frequently present on social media and is not necessarily exclusive to one group, topic, or real-world event. We discuss the implications of language as a powerful tool to engage, recruit, and radicalize those within communities online.
Multiple Levels of Heuristic Reasoning Processes in Scientific Model Construction
Authors: Clement John J.
Published: May 2022

Science historians have recognized the importance of heuristic reasoning strategies for constructing theories, but their extent and degree of organization are still poorly understood. This paper first consolidates a set of important heuristic strategies for constructing scientific models from three books, including studies in the history of genetics and electromagnetism, and an expert think-aloud study in the field of mechanics. The books focus on qualitative reasoning strategies (processes) involved in creative model construction, scientific breakthroughs, and conceptual change. Twenty four processes are examined, most of which are field-general, but all are heuristic in not being guaranteed to work. An organizing framework is then proposed as a four-level hierarchy of nested reasoning processes and subprocesses at different size and time scales, including: Level (L4) Several longer-time-scaleMajor Modeling Modes, such as Model Evolution and Model Competition; the former mode utilizes: (L3)Modeling Cycle Phasesof Model Generation, Evaluation, and Modification under Constraints;which can utilize: (L2) ThirteenTactical Heuristic Processes, e.g., Analogy, Infer new model feature (e.g., by running the model), etc.; many of which selectively utilize: (L1)Grounded Imagistic Processes, namely Mental Simulations and Structural Transformations. Incomplete serial ordering in the framework gives it an intermediate degree of organization that is neither anarchistic nor fully algorithmic. Its organizational structure is hypothesized to promote a difficult balance between divergent and convergent processes as it alternates between them in modeling cycles with increasingly constrained modifications. Videotaped think-aloud protocols that include depictive gestures and other imagery indicators indicate that the processes in L1 above can be imagistic. From neurological evidence that imagery uses many of the same brain regions as actual perception and action, it is argued that these expert reasoning processes are grounded in the sense of utilizing the perceptual and motor systems, and interconnections to and possible benefits for reasoning processes at higher levels are examined. The discussion examines whether this grounding and the various forms of organization in the framework may begin to explain how processes that are only sometimes useful and not guaranteed to work can combine successfully to achieve innovative scientific model construction.
Privacy Concerns and Information Sharing: The Perspective of the U-Shaped Curve
Authors: Hsu Chien-Lung , Liao Yi-Chuan , Lee Ching-Wen , Chan Lin Kun
Published: May 2022

Privacy concerns are a key predictor of information sharing, yet some critical issues remain unclear. Based on social capital theory, this study argues that the relationship between privacy concerns and information sharing is a U-shaped curve. Users with privacy concerns would not share their private information; however, such users would eventually share their information as long as they trust the website and its members. Furthermore, this study provides a contingency perspective, suggesting that the curvilinear relationship between privacy concerns and information sharing varies with the system evaluation perception and personal motivation levels. The results show that at a high level of system evaluation, the relationship between privacy concerns squared and information sharing is non-significant. In contrast, at a low level of system evaluation, there is a U-shaped relationship between privacy concerns and information sharing. Regarding motivation, the results were congruent with our expectations.
Through Thick and Thin: Changes in Creativity During the First Lockdown of the COVID-19 Pandemic
Authors: Lopez-Persem Alizée , Bieth Théophile , Guiet Stella , Ovando-Tellez Marcela , Volle Emmanuelle
Published: May 2022

COVID-19 took us by surprise. We all had to face the lockdown and pandemic that put us in a new context, changing our way of life, work conditions, and habits. Coping with such an unprecedented situation may have stimulated creativity. However, the situation also restricted our liberties and triggered health or psychological difficulties. We carried out an online survey (n = 380) to examine whether and how the COVID-19 related first lockdown period was associated with creativity changes in French speaking population. Despite a global negative subjective experience of the situation, participants reported that they were more creative during the lockdown than before. Positive changes were linked with more time availability, more motivation, or the need to solve a problem while negative changes were related to negative affective feelings or a lack of resources or opportunities. This study documents the effects of the first lockdown period on creativity and the factors that influenced it.
Enhanced Dichotic Listening and Temporal Sequencing Ability in Early-Blind Individuals
Authors: Bae Eun Bit , Jang Hyunsook , Shim Hyun Joon
Published: May 2022

Several studies have reported the better auditory performance of early-blind subjects over sighted subjects. However, few studies have compared the auditory functions of both hemispheres or evaluated interhemispheric transfer and binaural integration in blind individuals. Therefore, we evaluated whether there are differences in dichotic listening, auditory temporal sequencing ability, or speech perception in noise (all of which have been used to diagnose central auditory processing disorder) between early-blind subjects and sighted subjects. The study included 23 early-blind subjects and 22 age-matched sighted subjects. In the dichotic listening test (three-digit pair), the early-blind subjects achieved higher scores than the sighted subjects in the left ear (p = 0.003, Bonferroni’s corrected α = 0.05/6 = 0.008), but not in the right ear, indicating a right ear advantage in sighted subjects (p < 0.001) but not in early-blind subjects. In the frequency patterning test (five tones), the early-blind subjects performed better (both ears in the humming response, but the left ear only in the labeling response) than the sighted subjects (p < 0.008, Bonferroni’s corrected α = 0.05/6 = 0.008). Monosyllable perception in noise tended to be better in early-blind subjects than in sighted subjects at a signal-to-noise ratio of –8 (p = 0.054), the results at signal-to-noise ratios of –4, 0, +4, and +8 did not differ. Acoustic change complex responses to/ba/in babble noise, recorded with electroencephalography, showed a greater N1 peak amplitude at only FC5 electrode under a signal-to-noise ratio of –8 and –4 dB in the early-blind subjects than in the sighted subjects (p = 0.004 and p = 0.003, respectively, Bonferroni’s corrected α = 0.05/5 = 0.01). The results of this study revealed early-blind subjects exhibited some advantages in dichotic listening, and temporal sequencing ability compared to those shown in sighted subjects. These advantages may be attributable to the enhanced activity of the central auditory nervous system, especially the right hemisphere function, and the transfer of auditory information between the two hemispheres.
Career Calling as the Mediator and Moderator of Job Demands and Job Resources for Job Satisfaction in Health Workers: A Cross-Sectional Study
Authors: Huang Xianhong , Chen Hanlin , Gao Yuan , Wu Jin , Ni Ziling , Wang Xiaohe , Sun Tao
Published: May 2022

Job satisfaction of health professionals is a key determinant of the quality of health services and even affects the development of the healthcare system. In this study, we sought to explore the mechanism by which job demands, job resources, and career calling affect the job satisfaction of health professionals. Our findings may provide insights for increasing their job satisfaction and improving the quality of health services. We conducted a questionnaire survey of 1,117 health workers in Hangzhou; t-test, Chi-squared analysis, hierarchical linear regression was used to analyze the state of job satisfaction of health personnel and the associated factors; path analysis with the Structural Equation Model was used to explore and verify the effects of job resources, demands, and career calling on job satisfaction, as well as their mechanism. Social support, performance feedback, working conditions, and career calling had significant positive effects on job satisfaction of health professionals, whereas work-family conflict and emotional requirements for work had significant negative effects. Path analysis indicated that job resources, demands, and career calling directly affected job satisfaction; job resources and demands showed indirect effects on job satisfaction with career calling as a mediator. Career calling had a positive moderating effect in the path of “job resources–job satisfaction,” and a negative moderating effect in the path of “job demands–job satisfaction.” In conclusion, hospital administrators should provide more job resources for health workers and formulate reasonable job demands while paying close attention to work-related pressure. Hospital administrators and health departments need to improve hospital policies and inculcate a sense of belonging and career calling among health professionals. Education and evaluation of career calling need to be accorded more attention so that healthcare workers can perceive a stronger sense of calling and achievement, and hence a higher degree of job satisfaction.
Implicit Causality and Pronoun Resolution in Intersubjective Discourse Relations
Authors: Lyu Siqi , Wang Luming
Published: May 2022

Interpersonal verbs likedisappointandpraiseinLucy disappointed/praised Mary because she…bias the potential cause of the event to one of the antecedent noun phrases (henceforth NPs) (e.g., Lucy fordisappointwhereas Mary forpraise). Using Chinese as its materials, this study investigated how verb-based implicit causality affects online pronoun resolution in backward concession (e.g.,Lucy disappointed/praised Mary although she…), an intersubjective discourse relation where the subordinatealthough-clause forms an indirect relationship with the preceding main clause. Experiment 1 was a baseline experiment with the typical structure where implicit causality is found to be effective, i.e., backward causality. Results showed a clear modulation effect of implicit causality on pronoun resolution such that as verb bias strength decreases, participants were faster in processing sentences that disambiguate the pronoun to the verb-inconsistent NP. However, this modulation effect was not observed in Experiment 2 where we used the same verbs but replacedbecausewithalthough. There was no preference for the pronoun to be disambiguated toward the verb-consistent NP or the verb-inconsistent NP in backward concession. The results of Experiments 1 and 2 were replicated in Experiment 3 where we directly compared causal and concessive relations. We suggest that the absent effect of verb-based implicit causality in backward concession could be attributed to the intersubjective nature of the concessive relation. Discourse devices such asalthoughindicate speakers’ subjective perspective and comprehenders are able to quickly accommodate the speaker’s point of view during online discourse processing.
Increased Asymmetric Perfusion of the Cerebral Cortices and Thalamus Indicates Individuals at Risk for Bipolar Disorder: A Family Cohort Single Photon Emission Computed Tomography Neuroimaging Study
Authors: McLean Mary , Henderson Theodore A. , Pavel Dan G. , Cohen Phil
Published: May 2022

Bipolar disorder is a significant mental illness affecting over 4 million people in North America and approximately 46 million worldwide. While the onset of bipolar disorder is typically in late adolescence and early adulthood, the correct diagnosis can be delayed for several years. This delay can result in inappropriate pharmaceutical interventions, loss of career or productivity, suicide, family hardship, and unnecessary expense. Moreover, prolonged untreated or inappropriately treated bipolar disorder may cause damage to the brain. Early diagnosis is a critical need to circumvent the damage, suffering, and expense caused by the current delay. Brain perfusion single photon emission computed tomography (SPECT) neuroimaging reveals visual correlates of brain function. Herein, a family cohort all with bipolar disorder is described and their symptoms correlated with findings on the individual SPECT brain scans. The family consisted of two parents and three children (one female). The scans were interpreted by a panel of experts. Then a post hoc region-of-interest (ROI) analysis was conducted on SPECT data normalized to the cerebellum maximum with comparison to similarly normalized data from a normative sample. These findings support two distinct patterns of SPECT perfusion scan changes that can be found in individuals with bipolar disorder. In addition, these findings indicate that SPECT scan findings may be predictive of individual risk for progressing to symptomatic bipolar disorder. While preliminary, the findings in this cohort support the need for larger, diverse cohort studies of bipolar and control subjects to assess the predictive value of these particular SPECT perfusion findings in bipolar disorder.
Risk of Dental Caries and Erosive Tooth Wear in 117 Children and Adolescents' Anorexia Nervosa Population—A Case-Control Study
Authors: Paszynska Elzbieta , Hernik Amadeusz , Slopien Agnieszka , Roszak Magdalena , Jowik Katarzyna , Dmitrzak-Weglarz Monika , Tyszkiewicz-Nwafor Marta
Published: May 2022

Introduction

Restrictive type of anorexia nervosa (AN) is still one of the most severe eating disorders worldwide with an uncertain prognosis. Patients affected by AN should be encouraged to undertake psychiatric care and psychotherapy, but whether they should necessarily be included in careful dental care or not may still be questionable. Even though there is a constantly increasing number of AN studies, there are just a few data about the youngest group of AN children and adolescents aged < 18.

Methodology

This case-control study aimed to compare the dental health and gingival inflammation level in female adolescent inpatients affected by severe AN restrictive subtype vs. controls. Based on clinically confirmed 117 AN cases (hospitalized in years 2016–2020 in public Psychiatric Unit, BMI < 15 kg/m2, mean age 14.9 ± 1.8), the dental status has been examined regarding the occurrence of caries lesions using Decay Missing Filling Teeth (DMFT), erosive wear as Basic Erosive Wear Examination (BEWE), gingival condition as Bleeding on Probing (BOP) and plaque deposition as Plaque Control Record (PCR). The results were compared with age-matched 103 female dental patients (BMI 19.8 ± 2.3 kg/m2, age 15.0 ± 1.8, p = 0.746) treated in a public University dental clinic.

Results

AN patients were found to present a higher incidence of oral-related complications according to dental status (DMFT 3.8 ± 4.5 vs. 1.9 ± 2.1, p = 0.005), erosive tooth wear (BEWE 18.9 vs. 2.9%, p < 0.001), less efficient in controlling plaque (PCR 43.8 vs. 13.7%, p < 0.001) and gingival inflammation (BOP 20.0 vs. 3.9%, p < 0.001) compared with female adolescents. In the AN group, a significant correlation between BOP, BEWE, and duration of AN disease (p < 0.05), similarly to the number of decayed teeth D, filled teeth F and PCR were detected (p < 0.05).

Conclusions

Although the obtained results did not reveal any severe oral status, our findings indicated impaired dental and gingival conditions in young anorexics. Considering AN's potential role in oral health, it is essential to monitor dental treatment needs and oral hygiene levels in their present status to prevent forward complications in the future.
Development and Validation of a Rapid Assessment Version of the Assessment Survey of Primary Care in China
Authors: Zhong Chenwen , Huang Junjie , Li Lina , Luo Zhuojun , Liang Cuiying , Zhou Mengping , Kuang Li
Published: May 2022

Background

Measuring quality of primary care has attracted much attention around the world. Our team has developed and validated an Assessment Survey of Primary Care (ASPC) for evaluating quality of primary care in China. To facilitate the daily use of ASPC, this study aimed to develop and validate a rapid assessment version of ASPC (RA-ASPC) in China.

Methods

This is a multi-phase study on 21 experts and 1,184 patients from 12 primary care facilities in 10 cities in China. Importance, representativeness, easy understanding, and general applicability of each item in ASPC scale were rated to select the top two ranked items for constituting RA-ASPC. Reliability of RA-ASPC was tested by calculating both Cronbach's alpha and McDonald's omega coefficients. Structural validity was assessed by exploratory and confirmatory factor analysis (EFA and CFA). Concurrent validity was performed by analyzing the relationship between RA-ASPC and patient satisfaction. Discriminant validity was tested by assessing the difference of RA-ASPC scores between patients with or without family doctors.

Results

Ten items were selected for RA-ASPC. Both Cronbach's alpha (0.732) and McDonald's omega (0.729) suggested satisfactory internal consistency. In EFA, explained variance of RA-ASPC (72.6%) indicated its ability to measure quality of primary care in China. CFA indicators showed convincing goodness-of-fit (GFI = 0.996, AGFI = 0.992, CFI = 1.000, NFI = 0.980, RMR = 0.022, and the RMSEA = 0.000) for RA-ASPC. Positive association between RA-ASPC and patient satisfaction supported the concurrent validity of RA-ASPC. Patients with family doctors perceived higher quality of primary care than those without family doctors, indicating good discriminant validity of RA-ASPC.

Conclusion

The theoretical framework of RA-ASPC was in line with internationally recognized core functions of primary care. Good psychometric properties of RA-ASPC proved its appropriateness in assessing quality of primary care from patients' perspectives in China.
Empirical Study of Monthly Economic Losses Assessments for “Standard Unit Lockdown” Due to COVID-19
Authors: Zhang Houli , You Shibing , Zhang Miao , Chen Anqi , Hu Zengyun , Liu Ying , Liu Difei , Yuan Pei , Tan Yi
Published: May 2022

Background

The pandemic of COVID-19 has been shaping economic developments of the world. From the standpoint of government measures to prevent and control the epidemic, the lockdown was widely used. It is essential to access the economic losses in a lockdown environment which will provide government administration with a necessary reference for decision making in controlling the epidemic.

Methods

We introduce the concept of “standard unit incident” and an economic losses assessment methodology for both the standard and the assessed area. We build a “standard unit lockdown” economic losses assessment system and indicators to estimate the economic losses for the monthly lockdown. Using the comprehensive assessment system, the loss infected coefficient of monthly economic losses during lockdown in the 40 countries has been calculated to assess the economic losses by the entropy weighting method (EWM) with data from the CSMAR database and CDC website.

Results

We observe that countries in North America suffered the most significant economic losses due to the epidemic, followed by South America and Europe, Asia and Africa, and Oceania and Antarctica suffered relatively minor economic losses. The top 10 countries for monthly economic losses during lockdown were the United States, India, Brazil, France, Turkey, Russia, the United Kingdom, Italy, Spain, and Germany. The United States suffered the greatest monthly economic losses under lockdown ($65.3 billion), roughly 1.5 times that of China, while Germany suffered the least ($56.4 billion), roughly 1.3 times that of China.

Conclusion

Lockdown as a control and mitigation strategy has great impact on the economic development and causes huge economic losses. The economic impact due to the pandemic has varied widely among the 40 countries. It will be important to conduct further studies to compare and understand the differences and the reasons behind.
Social, Environmental and Economic Impact Assessment of COVID-19 on Rural Tourism
Authors: Eslami Fatemeh , Namdar Razieh
Published: May 2022

Today, various solutions have been proposed to improve the economic situation of villages and deprived areas, among which tourism is known as the best solution for those areas with the necessary potentials for tourism development. On other hand, the COVID-19 pandemic had significant effects on human life worldwide. The prevalence of COVID-19 has caused a lot of damage to different sectors of the global economy, but without a doubt, the rural tourism industry should be considered among the economic activities that have suffered the most from this virus. In this study, with the aim of investigating these effects on the rural tourism industry, it has been analyzed and compared in three important economic, social and environmental dimensions before and after the outbreak of the COVID-19. This quantitative study was used survey method. The statistical population of the study consisted of local stakeholders of rural tourism and experts of the relevant organizations in Natanz county of Iran. The results of confirmatory factor analysis indicate that the constructs used in the model have appropriate and acceptable fit. The results of the study also, showed that the prevalence of COVID-19 has adverse consequences including reducing the desirable economic and social effects of tourism mentioned among both groups of experts and rural stakeholders. from rural tourism stakeholders' opinion, environmental variables of the tourism areas before and after the COVID-19 was different, and in the absence of tourists in this area, the destructive environmental effects have strongly decreased.
Research on Diagnosis and Management of Postgraduates Mental Health Status Based on BP Neural Network
Authors: Xie Jiangning
Published: May 2022

As a special group facing the huge pressure of study, employment, life, and other aspects, graduate students have had fierce psychological conflicts. Their mental health status has been greatly affected. Postgraduate mental health problems are related to students' normal life and study and the scientific research work during graduate study. Because of this, this paper uses the BP neural network to establish a diagnostic model for postgraduate mental health status with the dependent variables of SCL-90 psychometric test results to realize the diagnosis of postgraduate' mental health status. The accuracy of the training, validation, and test process of the BP neural network is 98.448, 97.373, 98.128%, and the overall fitting accuracy of the model is 98.273% and the prediction accuracy of graduate mental health is 97.93%. Finally, according to the mental health status of graduate students to ensure the smooth progress of scientific research during graduate students.
Multiple E-Region Radar Propagation Modes Measured by the VHF SIMONe Norway System During Active Ionospheric Conditions
Authors: Huyghebaert Devin , Clahsen Matthias , Chau Jorge L. , Renkwitz Toralf , Latteck Ralph , Johnsen Magnar G. , Vierinen Juha
Published: May 2022

Multiple propagation modes between different bistatic radar links were measured during the operations of a very high frequency (VHF) 32.55 MHz radar system in northern Norway. The Spread Spectrum Interferometric Multistatic meteor radar Observing Network (SIMONe) Norway system detected meteor trails, direct transmitter to receiver signal propagation, over-the-horizon signal propagation from the SIMONe Germany system, ground and/or sea scatter, and ionospheric scatter on 27 August 2021 between 16:30–20:00 UT. These simultaneous detections were during an active ionospheric period with multiple occurrences of energetic charged particle precipitation. The SIMONe systems used continuous-wave (CW) pseudo-random phase modulated transmit signals and interferometry to make it possible to isolate each of these propagation modes and examine their characteristics. Different multistatic links at three receiver locations were analyzed, providing multistatic measurements of the regions with spatial and temporal resolutions on the order of 1.5 km and 2 s. The analysis techniques are described, with characteristics of the radar signal presented for each propagation mode and multistatic link. This study serves to highlight the capabilities of the SIMONe Norway system to research multiple aspects of ionospheric phenomena, specifically in the lower thermosphere-mesosphere boundary region.
Effect of Biochar on Micronutrient Availability and Uptake Into Leafy Greens in Two Urban Tropical Soils With Contrasting Soil pH
Authors: Rodríguez-Vila Alfonso , Atuah Laura , Abubakari Abdul Halim , Atorqui Dickson Worlanyo , Abdul-Karim Alhassan , Coole Sean , Hammond John , Robinson Steve , Sizmur Tom
Published: May 2022

Biochars have been proposed as a novel biotechnology to increase crop yields in acidic soils due to a liming effect. However, the application of biochar to soils with a neutral soil pH is less likely to improve yield. A rise in pH typically increases the availability of macronutrients (e.g., PO43-, NO3-) but biochar is known to immobilize some elements due to a pH increase and adsorption on the biochar surface. Therefore, biochar application may reduce the uptake of important micronutrients (e.g., Cu, Fe, and Zn) into the edible portions of food crops. Before recommending indiscriminate biochar application to tropical soils, an understanding of the potentially negative impacts of biochar application to contrasting soil types should be fully appreciated to prevent unintended consequences. Our aim was to determine the impact of biochar amendment to an acidic soil and a neutral soil on micronutrient availability and uptake into leafy greens. We produced biochars from 3 different organic feedstock materials (corn cobs, rice husk and teak sawdust) and applied these in pot experiments to an acidic tropical soil (pH 4.5) and a neutral tropical soil (pH 6.9) collected from urban farms in Tamale and Kumasi, respectively, in Ghana. We grew leafy greens (Amaranthus, Corchorus, and Lettuce) and measured their growth and the uptake of Cu, Fe, and Zn, alongside supporting measurements of soil pH and micronutrient availability in the soil. We also measured water soluble Cu, Fe, and Zn in the soils amended with biochars pyrolyzed at different temperatures. The corn cobs biochar increased soil pH and considerably increased plant growth in the acidic soil from Tamale. In the neutral soil from Kumasi we found that, while corn cob biochar increased soil pH, rice husk biochar decreased soil pH. Furthermore, corn cob biochar considerably reduced plant growth in the neutral soil. The concentration of micronutrients in the edible portions of leafy greens was not greatly affected by biochar application, but the total uptake (i.e., concentration multiplied by biomass) of micronutrients into leaves was generally increased by biochar application in the acidic (Tamale) soil and application of the corn cob biochar generally decreased total uptake of micronutrients in the neutral (Kumasi) soil. Our results highlight the need for site-specific information on biochar feedstock and soil pH prior to recommending biochar application to tropical urban soils so that the benefits can be optimized and unintended consequences can be prevented.
A New Antirotation Strategy of K-Wire Tension Band Therapy for Patellar Fracture
Authors: Sun Fengpo , Zhang Yawen , Ji Quan , Zhang Tongyi , Zhu Yi , Zhang Ze , Han Ruining , Wen Liangyuan
Published: May 2022

Background

Patellar fracture is a common phenomenon observed in orthopedic clinics. Many methods have been shown to be effective in the fixation of patellar fracture. However, there are few studies on the antirotation effect of these methods. The purpose of this study is to present a new strategy of K-wire tension band therapy for patellar fracture and explore the antirotation effect of the modified tension band method on patellar fracture.

Methods

A retrospective clinical observation study was conducted on 75 patients with patellar fracture. Totally, 46 patients were enrolled to the traditional group, who received the traditional K-wire tension band therapy. The modified group included 29 patients on whom our new strategy was implemented. The operation time, intraoperative blood loss, and fracture healing time were collected to compare the two operations and the knee society score (KSS) scores after the operations, and complications were recorded and retrieved to indicate the effectiveness of the two treatments.

Results

The preoperative baseline data (gender, age, fracture types) of the two groups showed no significant statistical difference. Similarly, there was no significant difference in the operation time, intraoperative blood loss, and fracture healing time between the two groups. The KSS clinical scores 1 year after operation was 90 (84, 95) for the traditional group as compared with 99 (97, 100) for the modified group (p < 0.05). The KSS functional scores 1 year after operation in the two groups were 90 (65, 90) and 100 (90, 100) (p < 0.05). The incidences of complications due to the rotation of K-wires in the traditional group and the modified group were 76.1% (35 of 46) and 6.9% (2 of 29) with a significant statistical difference (p < 0.05).

Conclusion

This study shows that our modified tension band therapy is an effective strategy for antirotation in the treatment of patellar fracture and proves that it can achieve better clinical outcomes than the traditional K-wire tension band method. This new strategy may be a safe and effective clinical technique for the treatment of patellar fracture. However, more prospective randomized controlled trials with larger sample sizes are still needed to further prove its efficacy.
Novel Retrograde Tibial Intramedullary Nailing for Distal Tibial Fractures
Authors: Peng Bin , Wan Teng , Tan Wenfu , Guo Weiming , He Min
Published: May 2022

Purpose

Postoperative distal tibial fractures are often associated with complications such as difficulties in fracture healing and surgical incision infection. The purpose of this study is to evaluate the fracture healing time and functional recovery after a retrograde tibial intramedullary nail treatment for distal tibial fractures.

Methods

We retrospectively studied 9 cases of patients with distal tibial fractures treated with retrograde intramedullary nailing (IMN). Fracture healing time was based on monthly postoperative x-ray imaging results, and functional outcomes were defined according to the American Orthopaedic Foot and Ankle Society (AOFAS) ankle-hindfoot score.

Results

Among the 9 patients with distal tibial fractures from July 2020 to April 2021, the mean age was 51.8 ± 13.8 years. The classification of distal tibial fractures in the 9 patients according to OTA (Orthopaedic Trauma Association) includes 6 extra-articular fractures (3 of type A1, 2 of type A2, and 1 of type A3) and 3 intra-articular fractures (1 of type C1 and 2 of type C2). Among them, there were 5 closed fracture cases and 4 open fracture cases (according to Gustilo classification: 2 of type I, 2 of type II). We treated the fractures surgically with new retrograde tibial intramedullary nailing. The mean follow-up time for this group was 7.9 months (5–12 months). According to monthly postoperative radiographs performed to monitor fracture healing, the mean healing time was 3.3 months (3–4 months). Final postoperative function according to the AOFAS ankle-hindfoot score shows 6 excellent cases, 3 good cases. No serious complications such as postoperative infection, bone and internal fixation exposure, osteofascial compartment syndrome, or vascular nerve injury occurred.

Conclusion

The new retrograde tibial intramedullary nail (RTN) has some unique advantages, and its treatment of distal tibial fractures can achieve good efficacy, but further studies are still needed to verify it.