bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Chronic Exposure to Microcystin-LR on Kidney in Mice
Authors: Yi Xiping , Xu Shuaishuai , Huang Feiyu , Wen Cong , Zheng Shuilin , Feng Hai , Guo Jian , Chen Jihua , Feng Xiangling , Yang Fei
Published: December 2019

Microcystin-LR (MC-LR) is a potent hepatotoxin, but a few studies suggested that it might also induce nephrotoxicity. However, nephrotoxicity induced by prolonged oral exposure to MC-LR is unknown. The aim of this study was to evaluate the potential influence of MC-LR on the kidney in mice following chronic exposure to MC-LR. In this study, we evaluated the nephrotoxicity of MC-LR in mice drinking water at different concentrations (1, 30, 60, 90, and 120 μg/L) for 6 months for the first time. The results showed that the kidney weights and the kidney indexes of mice were not altered in the MC-LR treated mice, compared with the control group. In addition, the renal function indicators revealed that the serum creatinine (SCr) levels were not significant changes after exposure to MC-LR. The blood urea nitrogen (BUN) levels were markedly decreased after exposure to 90 and 120 μg/L MC-LR for 3 months. The BUN levels were lower than that of the control group after exposure to 120 μg/L MC-LR for 6 months. The histopathological investigation revealed enlarged renal corpuscles, widened of kidney tubules, and lymphocyte infiltration in the interstitial tissue and the renal pelvis after exposure to 60, 90, and 120 μg/L MC-LR. Consequently, our results suggested that long-term exposure to MC-LR might be one important risk of kidney injury, which will provide important clues for the prevention of renal impairment.
Impact of Adapting the Abbreviated Injury Scale (AIS)-2005 from AIS-1998 on Injury Severity Scores and Clinical Outcome
Authors: Hsu Shiun-Yuan , Wu Shao-Chun , Rau Cheng-Shyuan , Hsieh Ting-Min , Liu Hang-Tsung , Huang Chun-Ying , Chou Sheng-En , Su Wei-Ti , Hsieh Ching-Hua
Published: December 2019

Background: In recent years, several versions of the Abbreviated Injury Scale (AIS) were updated and published. It was reported that the codeset in the dictionary of AIS-2005 had significant change from that of AIS-1998. This study was designed to evaluate the potential impact of adapting the AIS-2005 codeset from the AIS-1998 in an established trauma system of a single level I trauma center. The patients’ outcome was measured in different Injury Severity Score (ISS) strata according to the double-coded injuries in a three-year period. Methods: The double-coded injuries sustained by 7520 trauma patients between 1 January, 2016, and 31 December, 2018, in a level I trauma center were used to compare the patient injury characteristics and outcomes between AIS-1998 and AIS-2005 and under different ISS strata, defined as <16 (mild to moderate injury), 16–24 (severe injury), and >24 (critical injury). Results: The mean ISS was significantly lower using AIS-2005 than using AIS-1998 (7.5 ± 6.3 vs. 8.3 ± 7.1, respectively, p < 0.001). AIS-2005 scores in the body regions of the head/neck (2.94 ± 1.08 vs. 3.40 ± 1.15, respectively, p < 0.001) and extremity (2.19 ± 0.56 vs. 2.24 ± 0.58, respectively, p < 0.001), but not in other body regions, were significantly lower than AIS-1998 scores. The critically injured patients (ISS >24), but not severely injured patients or patients with mild-to-moderate injury, coded by AIS-2005 had a significantly higher mortality rate (34.2% vs. 26.2%, respectively, p = 0.031) than did patients coded by AIS-1998. The rate of intensive care unit admission was significantly higher for patients in all ISS strata after adapting AIS-2005 as the scoring system than after adapting AIS-1998. Regarding patients with major trauma, which was defined as ISS > 15, the number of patients with major trauma in this study was 17.0% (n = 1276) for AIS-1998 and 9.7% (n = 733) for AIS-2005. As a consequence, the mortality rate of patients with major trauma was significantly higher in AIS-2005 than in AIS-1998 (15.4% vs. 9.1%, respectively, p < 000.1). Conclusions: In this study, we revealed that the adaptation of AIS-2005 from AIS-1998 had resulted in a significant decrease of severity scores in the measurement of the same injuries. The number of head/neck injuries classified as 16–24 was the key difference between AIS-1998 and AIS-2005. Furthermore, critically injured patients who had ISS > 24 coded by AIS-2005 had significantly higher mortality rates than did the patients coded by AIS-1998. This study also indicated that a direct comparison of the measurements that are generated from these two AIS versions can produce misleading results.
Using Geographic Ontologies and Geo-Characterization to Represent Geographic Scenarios
Authors: Huang Yi , Yuan May , Sheng Yehua , Min Xiangqiang , Cao Yuwei
Published: December 2019

Traditional Geographic Information Systems (GIS) represent the environment under reductionist thinking, which disaggregates a geographic environment into independent geographic themes. The reductionist approach makes the spatiotemporal characteristics of geo-features explicit, but neglects the holistic nature of the environment, such as the hierarchical structure and interactions among environmental elements. To fill this gap, we integrate the concept geographic scenario with the fundamental principles of General System Theory to realize the environmental complexity in GIS. With the integration, a geographic scenario constitutes a hierarchy of spatiotemporal frameworks for organizing environmental elements and subserving the exploration of their relationships. Furthermore, we propose geo-characterization with ontological commitments to both static and dynamic properties of a geographic scenario and prescribe spatial, temporal, semantic, interactive, and causal relationships among environmental elements. We have tested the utility of the proposed representation in OWL and the associated reasoning process in Semantic Web Rule Language (SWRL) rules in a case study in Nanjing, China. The case study represents Nanjing and the Nanjing presidential palace to demonstrate the connections among environmental elements in different scenarios and the support for information queries, evolution process simulation, and semantic inferences. The proposed representation encodes geographic knowledge of the environment, makes the interactions among environmental elements explicit, supports geographic process simulation, opens opportunities for deep knowledge mining, and grounds a foundation for GeoAI to discover geographic complexity and dynamics beyond the support of conventional theme-centric inquiries in GIS.
Polyelectrolyte-Nanoplatelet Complexation: Is It Possible to Predict the State Diagram?
Authors: Jansson Maria , Skepö Marie
Published: December 2019

The addition of polyelectrolytes (PEs) to suspensions of charged colloids, such as nanoplatelets (NPs), is of great interest due to their specific feature of being either a stabilizing or a destabilizing agent. Here, the complexation between a PE and oppositely charged NPs is studied utilizing coarse-grained molecular dynamics simulations based on the continuum model. The complex formation is evaluated with respect to the stoichiometric charge-ratio within the system, as well as by the alternation of the chain properties. It is found that the formed complexes can possess either an extended or a compact shape. Moreover, it is observed that the chain can become overcharged by the oppositely charged NPs. With an increase in chain length, or a decrease in chain flexibility, the complex obtains a more extended shape, where the NPs are less tightly bound to the PE. The latter is also true when reducing the total charge of the chain by varying the linear charge density, whereas in this case, the chain contracts. With our coarse-grained model and molecular dynamics simulations, we are able to predict the composition and the shape of the formed complex and how it is affected by the characteristics of the chain. The take-home message is that the complexation between PEs and NPs results in a versatile and rich state diagram, which indeed is difficult to predict, and dependent on the properties of the chain and the model used. Thus, we propose that the present model can be a useful tool to achieve an understanding of the PE-NPs complexation, a system commonly used in industrial and in technological processes.
The Retinoid and Non-Retinoid Ligands of the Rod Visual G Protein-Coupled Receptor
Authors: Ortega Joseph T. , Jastrzebska Beata
Published: December 2019

G protein-coupled receptors (GPCRs) play a predominant role in the drug discovery effort. These cell surface receptors are activated by a variety of specific ligands that bind to the orthosteric binding pocket located in the extracellular part of the receptor. In addition, the potential binding sites located on the surface of the receptor enable their allosteric modulation with critical consequences for their function and pharmacology. For decades, drug discovery focused on targeting the GPCR orthosteric binding sites. However, finding that GPCRs can be modulated allosterically opened a new venue for developing novel pharmacological modulators with higher specificity. Alternatively, focus on discovering of non-retinoid small molecules beneficial in retinopathies associated with mutations in rhodopsin is currently a fast-growing pharmacological field. In this review, we summarize the accumulated knowledge on retinoid ligands and non-retinoid modulators of the light-sensing GPCR, rhodopsin and their potential in combating the specific vision-related pathologies. Also, recent findings reporting the potential of biologically active compounds derived from natural products as potent rod opsin modulators with beneficial effects against degenerative diseases related to this receptor are highlighted here.
Influence of Lytic Polysaccharide Monooxygenase Active Site Segments on Activity and Affinity
Authors: Laurent Christophe V.F.P. , Sun Peicheng , Scheiblbrandner Stefan , Csarman Florian , Cannazza Pietro , Frommhagen Matthias , van Berkel Willem J.H. , Oostenbrink Chris , Kabel Mirjam A. , Ludwig Roland
Published: December 2019

In past years, new lytic polysaccharide monooxygenases (LPMOs) have been discovered as distinct in their substrate specificity. Their unconventional, surface-exposed catalytic sites determine their enzymatic activities, while binding sites govern substrate recognition and regioselectivity. An additional factor influencing activity is the presence or absence of a family 1 carbohydrate binding module (CBM1) connected via a linker to the C-terminus of the LPMO. This study investigates the changes in activity induced by shortening the second active site segment (Seg2) or removing the CBM1 from Neurospora crassa LPMO9C. NcLPMO9C and generated variants have been tested on regenerated amorphous cellulose (RAC), carboxymethyl cellulose (CMC) and xyloglucan (XG) using activity assays, conversion experiments and surface plasmon resonance spectroscopy. The absence of CBM1 reduced the binding affinity and activity of NcLPMO9C, but did not affect its regioselectivity. The linker was found important for the thermal stability of NcLPMO9C and the CBM1 is necessary for efficient binding to RAC. Wild-type NcLPMO9C exhibited the highest activity and strongest substrate binding. Shortening of Seg2 greatly reduced the activity on RAC and CMC and completely abolished the activity on XG. This demonstrates that Seg2 is indispensable for substrate recognition and the formation of productive enzyme-substrate complexes.
An Integrated Pan-Cancer Analysis and Structure-Based Virtual Screening of GPR15
Authors: Wang Yanjing , Wang Xiangeng , Xiong Yi , Li Cheng-Dong , Xu Qin , Shen Lu , Chandra Kaushik Aman , Wei Dong-Qing
Published: December 2019

G protein-coupled receptor 15 (GPR15, also known as BOB) is an extensively studied orphan G protein-coupled receptors (GPCRs) involving human immunodeficiency virus (HIV) infection, colonic inflammation, and smoking-related diseases. Recently, GPR15 was deorphanized and its corresponding natural ligand demonstrated an ability to inhibit cancer cell growth. However, no study reported the potential role of GPR15 in a pan-cancer manner. Using large-scale publicly available data from the Cancer Genome Atlas (TCGA) and the Genotype-Tissue Expression (GTEx) databases, we found that GPR15 expression is significantly lower in colon adenocarcinoma (COAD) and rectal adenocarcinoma (READ) than in normal tissues. Among 33 cancer types, GPR15 expression was significantly positively correlated with the prognoses of COAD, neck squamous carcinoma (HNSC), and lung adenocarcinoma (LUAD) and significantly negatively correlated with stomach adenocarcinoma (STAD). This study also revealed that commonly upregulated gene sets in the high GPR15 expression group (stratified via median) of COAD, HNSC, LUAD, and STAD are enriched in immune systems, indicating that GPR15 might be considered as a potential target for cancer immunotherapy. Furthermore, we modelled the 3D structure of GPR15 and conducted structure-based virtual screening. The top eight hit compounds were screened and then subjected to molecular dynamics (MD) simulation for stability analysis. Our study provides novel insights into the role of GPR15 in a pan-cancer manner and discovered a potential hit compound for GPR15 antagonists.
A Novel DES L115F Mutation Identified by Whole Exome Sequencing is Associated with Inherited Cardiac Conduction Disease
Authors: Hsu Lung-An , Ko Yu-Shien , Yeh Yung-Hsin , Chang Chi-Jen , Chan Yi-Hsin , Kuo Chi-Tai , Tsai Hsin-Yi , Chang Gwo-Jyh
Published: December 2019

Inherited cardiac conduction disease (CCD) is rare; it is caused by a large number of mutations in genes encoding cardiac ion channels and cytoskeletal proteins. Recently, whole-exome sequencing has been successfully used to identify causal mutations for rare monogenic Mendelian diseases. We used trio-based whole-exome sequencing to study a Chinese family with multiple family members affected by CCD, and identified a heterozygous missense mutation (c.343C>T, p.Leu115Phe) in the desmin (DES) gene as the most likely candidate causal mutation for the development of CCD in this family. The mutation is novel and is predicted to affect the conformation of the coiled-coil rod domain of DES according to structural model prediction. Its pathogenicity in desmin protein aggregation was further confirmed by expressing the mutation, both in a cellular model and a CRISPR/CAS9 knock-in mouse model. In conclusion, our results suggest that whole-exome sequencing is a feasible approach to identify candidate genes underlying inherited conduction diseases.
A Genetic Algorithm-Based Approach for Composite Metamorphic Relations Construction
Authors: Xiang Zhenglong , Wu Hongrun , Yu Fei
Published: December 2019

The test oracle problem exists widely in modern complex software testing, and metamorphic testing (MT) has become a promising testing technique to alleviate this problem. The inference of efficient metamorphic relations (MRs) is the core problem of metamorphic testing. Studies have proven that the combination of simple metamorphic relations can construct more efficient metamorphic relations. In most previous studies, metamorphic relations have been mainly manually inferred by experts with professional knowledge, which is an inefficient technique and hinders the application. In this paper, a genetic algorithm-based approach is proposed to construct composite metamorphic relations automatically for the program to be tested. We use a set of relation sequences to represent a particular class of MRs and turn the problem of inferring composite MRs into a problem of searching for suitable sequences. We then dynamically implement multiple executions of the program and use a genetic algorithm to search for the optimal set of relation sequences. We conducted empirical studies to evaluate our approach using scientific functions in the GNU scientific library (abbreviated as GSL). From the empirical results, our approach can automatically infer high-quality composite MRs, on average, five times more than basic MRs. More importantly, the inferred composite MRs can increase the fault detection capabilities by at least 30 % more than the original metamorphic relations.
Experimental and Numerical Analysis of the Hydrodynamics around a Vertical Cylinder in Waves
Authors: Corvaro Sara , Crivellini Andrea , Marini Francesco , Cimarelli Andrea , Capitanelli Loris , Mancinelli Alessandro
Published: December 2019

The present study provides an extensive analysis on the hydrodynamics induced by a vertical slender pile under wave action. The authors carried out the study both experimentally and numerically, thus enabling a deep understanding of the flow physics. The experiments took place at a wave flume of the Università Politecnica delle Marche. Two different experimental campaigns were performed: In the former one, a mobile bed model was realized with the aims to study both the scour process and the hydrodynamics around the cylinder; in the latter one, the seabed was rigid in order to make undisturbed optical measurements, providing a deeper analysis of the hydrodynamics. The numerical investigation was made by performing a direct numerical simulation. A second order numerical discretization, both in time and in space, was used to solve the Navier–Stokes equations while a volume of fluid (VOF) approach was adopted for tracking the free surface. The comparison between experimental and numerical results is provided in terms of velocity, pressure distributions around the cylinder, and total force on it. The analysis of the pressure gradient was used to evaluate the generation and evolution of vortices around the cylinder. Finally, the relation between scour and bed shear stresses due to the structure of the vortex pattern around the pile was assessed. It is worth noting that the physical understanding of this last analysis was enabled by the combined use of experimental data on scour and numerical data on the flow pattern.
Shipbuilding 4.0 Index Approaching Supply Chain
Authors: Ramirez-Peña Magdalena , Abad Fraga Francisco J. , Sánchez Sotano Alejandro J. , Batista Moises
Published: December 2019

The shipbuilding industry shows a special interest in adapting to the changes proposed by the industry 4.0. This article bets on the development of an index that indicates the current situation considering that supply chain is a key factor in any type of change, and at the same time it serves as a control tool in the implementation of improvements. The proposed indices provide a first definition of the paradigm or paradigms that best fit the supply chain in order to improve its sustainability and a second definition, regarding the key enabling technologies for Industry 4.0. The values obtained put shipbuilding on the road to industry 4.0 while suggesting categorized planning of technologies.
Effect of Irradiated and Non-Irradiated Waste PET Based Cementitious Grouts on Flexural Strength of Semi-Flexible Pavement
Authors: Khan Muhammad Imran , Huat Huang Yong , Dun Mohammad Haziq bin Muhamad , Sutanto Muslich Hartadi , Jarghouyeh Ehsan Nikbakht , Zoorob Salah E.
Published: December 2019

In this study the effect of irradiated and non-irradiated waste polyethylene terephthalate (PET) as replacement of cement and fly-ash in ordinary Portland cement (OPC) and geopolymeric cement (GPC) based cementitious grouts on flexural strength of semi-flexible pavement specimens were evaluated. The porous asphalt gradation was selected based on Malaysian specifications for semi-flexible pavements with a target of 30% air voids. The cement content in the OPC grouts and the fly-ash content in the GPC based grouts were partially replaced with 1.25% PET (using both irradiated and non-irradiated PET). Beam specimens were prepared and tested for flexural strength properties using center point loading configuration. The grouts modified with recycled waste plastic (PET) showed approximately the same results as obtained from the control specimens. Although the replacement amount was low (1.25% by weight of cement), nonetheless, significant impact on reducing CO2 emissions is expected when preparing grouts for mass construction of semi-flexible pavement surfaces. Similarly, effective recycling of waste plastics in road construction and replacing OPC with plastic and geopolymers will have a positive effect on the environment and will furthermore contribute to sustainable pavement construction.
Europium-Doped Tellurite Glasses: The Eu2+ Emission in Tellurite, Adjusting Eu2+ and Eu3+ Emissions toward White Light Emission
Authors: Elkholy Hagar , Othman Hosam , Hager Ibrahim , Ibrahim Medhat , de Ligny Dominique
Published: December 2019

Europium-doped magnesium tellurite glasses were prepared using melt quenching techniques and attenuated total reflection (ATR) spectroscopy was used to study the glass structure. The glass transition temperature increased with increasing MgO content. Eu2+ and Eu3+ emissions were studied using photoluminescence spectroscopy (PL). The broad emission of Eu2+ ions centered at approximately 485 nm was found to decrease in intensity with increasing MgO content, while the Eu3+ emission was enhanced. The Eu3+ emission lay within the red orange range and its decay time was found to increase with increasing MgO content. Different excitation wavelengths were used to adjust Eu2+ to Eu3+ emissions to reach white light emission. The white light emission was obtained for the sample with the lowest MgO content under excitation in the near-UV range.
Asymptotic Stability of Non-Autonomous Systems and a Generalization of Levinson’s Theorem
Authors: Lee Min-Gi
Published: December 2019

We study the asymptotic stability of non-autonomous linear systems with time dependent coefficient matrices { A ( t ) } t ∈ R . The classical theorem of Levinson has been an indispensable tool for the study of the asymptotic stability of non-autonomous linear systems. Contrary to constant coefficient system, having all eigenvalues in the left half complex plane does not imply asymptotic stability of the zero solution. Levinson’s theorem assumes that the coefficient matrix is a suitable perturbation of the diagonal matrix. Our objective is to prove a theorem similar to Levinson’s Theorem when the family of matrices merely admits an upper triangular factorization. In fact, in the presence of defective eigenvalues, Levinson’s Theorem does not apply. In our paper, we first investigate the asymptotic behavior of upper triangular systems and use the fixed point theory to draw a few conclusions. Unless stated otherwise, we aim to understand asymptotic behavior dimension by dimension, working with upper triangular with internal blocks adds flexibility to the analysis.
Ejaculatory and Orgasmic Dysfunction Following Prostate Cancer Therapy: Clinical Management
Authors: Green Travis P. , Saavedra-Belaunde Jose , Wang Run
Published: December 2019

The majority of sexual health research has focused on erectile dysfunction following prostate cancer treatment. Ejaculatory and orgasmic dysfunction are significant side effects following the treatment of prostate cancer. Orgasmic dysfunction covers a range of issues including premature ejaculation, anorgasmia, dysorgasmia, and climacturia. This review provides an overview of prevalence and management options to deal with orgasmic dysfunction. A Medline Pubmed search was used to identify articles relating to these problems. We found that orgasmic dysfunction has a very large impact on patients’ lives following prostate cancer treatment and there are ways for physicians to treat it. Management of patients’ sexual health should be focused not only on erectile dysfunction, but on orgasmic dysfunction as well in order to ensure a healthy sexual life for patients and their partners.
µ-PIV Measurements of Flows Generated by Photolithography-Fabricated Achiral Microswimmers
Authors: Tan Liyuan , Ali Jamel , Cheang U Kei , Shi Xiangcheng , Kim Dalhyung , Kim Min Jun
Published: December 2019

Robotic micro/nanoswimmers can potentially be used as tools for medical applications, such as drug delivery and noninvasive surgery. Recently, achiral microswimmers have gained significant attention because of their simple structures, which enables high-throughput fabrication and size scalability. Here, microparticle image velocimetry (µ-PIV) was used to study the hydrodynamics of achiral microswimmers near a boundary. The structures of these microswimmers resemble the letter L and were fabricated using photolithography and thin-film deposition. Through µ-PIV measurements, the velocity flow fields of the microswimmers rotating at different frequencies were observed. The results herein yield an understanding of the hydrodynamics of the L-shaped microswimmers, which will be useful in applications such as fluidic manipulation.
Multiple Sense and Antisense Promoters Contribute to the Regulated Expression of the isc-suf Operon for Iron-Sulfur Cluster Assembly in Rhodobacter
Authors: Nie Xin , Remes Bernhard , Klug Gabriele
Published: December 2019

A multitude of biological functions relies on iron-sulfur clusters. The formation of photosynthetic complexes goes along with an additional demand for iron-sulfur clusters for bacteriochlorophyll synthesis and photosynthetic electron transport. However, photooxidative stress leads to the destruction of iron-sulfur clusters, and the released iron promotes the formation of further reactive oxygen species. A balanced regulation of iron-sulfur cluster synthesis is required to guarantee the supply of this cofactor, on the one hand, but also to limit stress, on the other hand. The phototrophic alpha-proteobacterium Rhodobacter sphaeroides harbors a large operon for iron-sulfur cluster assembly comprising the iscRS and suf genes. IscR (iron-sulfur cluster regulator) is an iron-dependent regulator of isc-suf genes and other genes with a role in iron metabolism. We applied reporter gene fusions to identify promoters of the isc-suf operon and studied their activity alone or in combination under different conditions. Gel-retardation assays showed the binding of regulatory proteins to individual promoters. Our results demonstrated that several promoters in a sense and antisense direction influenced isc-suf expression and the binding of the IscR, Irr, and OxyR regulatory proteins to individual promoters. These findings demonstrated a complex regulatory network of several promoters and regulatory proteins that helped to adjust iron-sulfur cluster assembly to changing conditions in Rhodobacter sphaeroides.
Biochar Suppresses Bacterial Wilt of Tomato by Improving Soil Chemical Properties and Shifting Soil Microbial Community
Authors: Gao Yang , Lu Yang , Lin Weipeng , Tian Jihui , Cai Kunzheng
Published: December 2019

The role of biochar amendments in enhancing plant disease resistance has been well documented, but its mechanism is not yet fully understood. In the present study, 2% biochar made from wheat straw was added to the soil of tomato infected by Ralstonia solanacearum to explore the interrelation among biochar, tomato bacterial wilt resistance, soil chemical properties, and soil microbial community and to decipher the disease suppression mechanisms from a soil microbial perspective. Biochar application significantly reduced the disease severity of bacterial wilt, increased soil total organic carbon, total nitrogen, C:N ratio, organic matter, available P, available K, pH, and electrical conductivity. Biochar treatment also increased soil acid phosphatase activity under the non-R.-solanacearum-inoculated condition. High-throughput sequencing of 16S rRNA revealed substantial differences in rhizosphere bacterial community structures between biochar-amended and nonamended treatments. Biochar did not influence soil microbial richness and diversity but significantly increased the relative abundance of Bacteroidetes and Proteobacteria in soil at the phylum level under R. solanacearum inoculation. Furthermore, biochar amendment harbored a higher abundance of Chitinophaga, Flavitalea, Adhaeribacter, Pontibacter, Pedobacter, and Ohtaekwangia at the genus level of Bacteroides and Pseudomonas at the genus level of Proteobacteria under R. solanacearum inoculation. Our findings suggest that a biochar-shifted soil bacterial community structure can favorably contribute to the resistance of tomato plants against bacterial wilt.
Recognition of Late Triassic Cu-Mo Mineralization in the Northern Yidun Arc (S.E. Tibetan Plateau): Implications for Regional Exploration
Authors: Tian Zhen-Dong , Leng Cheng-Biao , Zhang Xing-Chun , Zhou Li-Min , Tang Yan-Wen
Published: December 2019

The Yidun arc, located in the southeastern Tibetan Plateau, was formed by the westward subduction of the Ganze-Litang Paleo-Tethys ocean in Late Triassic. It is well-known for the formation of numerous Mesozoic porphyry-skarn Cu-Mo-(Au) deposits in the arc. To date, more than 20 Cu-Mo-(Au) deposits (>10 million tonnes Cu resources) have been discovered in the southern Eastern Yidun arc. However, few Cu-Mo deposits have been discovered in the northern Eastern Yidun arc. In recent years, some Cu-Mo deposits or occurrence are successively discovered in the northern Eastern Yidun arc, but their ore-forming ages are not well constrained. It remains unclear whether such Cu-Mo mineralization formed by similar metallogenic event and geodynamic setting as the Cu-Mo-(Au) mineralization in the south. In order to determine the metallogenic age and shed light on potential links between Cu-Mo mineralization and regional magmatic events, we present molybdenite Re-Os and zircon U-Pb ages to constrain the timing of two types of Cu-Mo mineralization in the northern Eastern Yidun arc (type I and type II). Molybdenite ICP-MS Re-Os dating results show that type I mineralization was formed at 217.7 ± 3.6 Ma, which is highly consistent with the formation ages of the host granite (218.1 ± 1.5 Ma, 2σ, n = 15, MSWD = 0.92) and aplite dyke (217.3 ± 1.3 Ma, 2σ, n = 16, MSWD = 0.50) within error. While the type II mineralization has a relatively younger formation age of 211.8 ± 4.7 Ma than the host granite (217.1 ± 1.5 Ma, 2σ, n = 14, MSWD = 0.96) and type I Cu-Mo mineralization. These data indicate that the Cu-Mo mineralization in the northern Eastern Yidun arc was temporally and spatially related to the Late Triassic magmatism in the region. Rhenium (Re) concentrations in the molybdenite from type I mineralization, ranging from 12.77 to 111.1 ppm (typically > 100 ppm), indicate that the ore-forming metals were derived mainly from a mantle source. However, Re contents in molybdenite from the type II mineralization, ranging from 7.983 to 10.40 ppm, indicate that the ore-forming metals were derived from a mixed mantle and crustal source with a predominantly crustal component. This study confirms that the northern Eastern Yidun arc exists Late Triassic Cu-Mo metallogenesis, and thus much attention should be paid on this region to find more Late Triassic Cu-Mo resources.
Antiplasmodial Activity of Nitroaromatic Compounds: Correlation with Their Reduction Potential and Inhibitory Action on Plasmodium falciparum Glutathione Reductase
Authors: Marozienė Audronė , Lesanavičius Mindaugas , Davioud-Charvet Elisabeth , Aliverti Alessandro , Grellier Philippe , Šarlauskas Jonas , Čėnas Narimantas
Published: December 2019

With the aim to clarify the mechanism(s) of action of nitroaromatic compounds against the malaria parasite Plasmodium falciparum, we examined the single-electron reduction by P. falciparum ferredoxin:NADP+ oxidoreductase (PfFNR) of a series of nitrofurans and nitrobenzenes (n = 23), and their ability to inhibit P. falciparum glutathione reductase (PfGR). The reactivity of nitroaromatics in PfFNR-catalyzed reactions increased with their single-electron reduction midpoint potential (E17). Nitroaromatic compounds acted as non- or uncompetitive inhibitors towards PfGR with respect to NADPH and glutathione substrates. Using multiparameter regression analysis, we found that the in vitro activity of these compounds against P. falciparum strain FcB1 increased with their E17 values, octanol/water distribution coefficients at pH 7.0 (log D), and their activity as PfGR inhibitors. Our data demonstrate that both factors, the ease of reductive activation and the inhibition of PfGR, are important in the antiplasmodial in vitro activity of nitroaromatics. To the best of our knowledge, this is the first quantitative demonstration of this kind of relationship. No correlation between antiplasmodial activity and ability to inhibit human erythrocyte GR was detected in tested nitroaromatics. Our data suggest that the efficacy of prooxidant antiparasitic agents may be achieved through their combined action, namely inhibition of antioxidant NADPH:disulfide reductases, and the rapid reduction by single-electron transferring dehydrogenases-electrontransferases.
Highly Sensitive Detection of the Antibiotic Ciprofloxacin by Means of Fiber Enhanced Raman Spectroscopy
Authors: Wolf Sebastian , Frosch Timea , Popp Juergen , Pletz Mathias W. , Frosch Torsten
Published: December 2019

Sepsis and septic shock exhibit a rapid course and a high fatality rate. Antibiotic treatment is time-critical and precise knowledge of the antibiotic concentration during the patients’ treatment would allow individual dose adaption. Over- and underdosing will increase the antimicrobial efficacy and reduce toxicity. We demonstrated that fiber enhanced Raman spectroscopy (FERS) can be used to detect very low concentrations of ciprofloxacin in clinically relevant doses, down to 1.5 µM. Fiber enhancement was achieved in bandgap shifted photonic crystal fibers. The high linearity between the Raman signals and the drug concentrations allows a robust calibration for drug quantification. The needed sample volume was very low (0.58 µL) and an acquisition time of 30 s allowed the rapid monitoring of ciprofloxacin levels in a less invasive way than conventional techniques. These results demonstrate that FERS has a high potential for clinical in-situ monitoring of ciprofloxacin levels.
Preparative Method for Asymmetric Synthesis of (S)-2-Amino-4,4,4-trifluorobutanoic Acid
Authors: Han Jianlin , Takeda Ryosuke , Liu Xinyi , Konno Hiroyuki , Abe Hidenori , Hiramatsu Takahiro , Moriwaki Hiroki , Soloshonok Vadim A.
Published: December 2019

Enantiomerically pure derivatives of 2-amino-4,4,4-trifluorobutanoic acid are in great demand as bioisostere of leucine moiety in the drug design. Here, we disclose a method specifically developed for large-scale (>150 g) preparation of the target (S)-N-Fmoc-2-amino-4,4,4-trifluorobutanoic acid. The method employs a recyclable chiral auxiliary to form the corresponding Ni(II) complex with glycine Schiff base, which is alkylated with CF3–CH2–I under basic conditions. The resultant alkylated Ni(II) complex is disassembled to reclaim the chiral auxiliary and 2-amino-4,4,4-trifluorobutanoic acid, which is in situ converted to the N-Fmoc derivative. The whole procedure was reproduced several times for consecutive preparation of over 300 g of the target (S)-N-Fmoc-2-amino-4,4,4-trifluorobutanoic acid.
Upgrading of Extra-Heavy Crude Oils by Dispersed Injection of NiO–PdO/CeO2±δ Nanocatalyst-Based Nanofluids in the Steam
Authors: Medina Oscar E. , Caro-Vélez Cristina , Gallego Jaime , Cortés Farid B. , Lopera Sergio H. , Franco Camilo A.
Published: December 2019

The main objective of this study is to evaluate the injection of a dispersed nanocatalyst-based nanofluid in a steam stream for in situ upgrading and oil recovery during a steam injection process. The nanocatalyst was selected through adsorption and thermogravimetric experiments. Two nanoparticles were proposed, ceria nanoparticles (CeO2±δ), with and without functionalization with nickel, and palladium oxides (CeNi0.89Pd1.1). Each one was employed for static tests of adsorption and subsequent decomposition using a model solution composed of n-C7 asphaltenes (A) and resins II (R) separately and for different R:A ratios of 2:8, 1:1, and 8:2. Then, a displacement test consisting of three main stages was successfully developed. At the beginning, steam was injected into the porous media at a temperature of 210 °C, the pore and overburden pressure were fixed at 150 and 800 psi, respectively, and the steam quality was 70%. This was followed by CeNi0.89Pd1.1 dispersed injection in the steam stream. Finally, the treatment was allowed to soak for 12 h, and the steam flooding was carried out again until no more oil production was observed. Among the most relevant results, functionalized nanoparticles achieved higher adsorption of both fractions as well as a lower decomposition temperature. The presence of resins did not affect the amount of asphaltene adsorption over the evaluated materials. The catalytic activity suggests that the increase in resin content promotes a higher conversion in a shorter period of time. Also, for the different steps of the dynamic test, increases of 25% and 42% in oil recovery were obtained for the dispersed injection of the nanofluid in the steam stream and after a soaking time of 12 h, compared with the base curve with only steam injection, respectively. The upgraded crude oil reached an API gravity level of 15.9°, i.e., an increase in 9.0° units in comparison with the untreated extra-heavy crude oil, which represents an increase of 130%. Also, reductions of up to 71% and 85% in the asphaltene content and viscosity were observed.
Effect of Fibre-Enriched Orange Juice on Postprandial Glycaemic Response and Satiety in Healthy Individuals: An Acute, Randomised, Placebo-Controlled, Double-Blind, Crossover Study
Authors: Bosch-Sierra Neus , Marqués-Cardete Roger , Gurrea-Martínez Aránzazu , Grau-Del Valle Carmen , Talens Clara , Alvarez-Sabatel Saioa , Bald Carlos , Morillas Carlos , Hernández-Mijares Antonio , Bañuls Celia
Published: December 2019

Background: Consumption of fibre-enriched orange juice may be an appropriate way to supplement daily fibre intake and achieve beneficial effects on metabolic health. The present study aimed to assess the short-term effects of fibre-enriched orange juice on postprandial metabolism and satiety in a healthy adult population. Methods: In this double-blind, randomised, placebo-controlled, crossover study 10 healthy subjects underwent two one-day trials in which they consumed an orange juice beverage containing 1.4 g/100 mL of citrus fibre (29.3% soluble and 41.9% insoluble) or a placebo (regular orange juice without added fibre). Postprandial glucose, insulin, gut hormones (GLP1, GIP and ghrelin), leptin and qualitative appetite/satiety assessment were measured every 15 or 30 min over the 120 min test period. Results: The fibre-enriched orange juice decreased postprandial serum glucose and circulating insulin levels at 15 min compared with the placebo. In addition, after intake of the fibre-enriched juice, a significant effect on qualitative feelings of satiety and fullness was observed at 15 and 120 min, and was accompanied by a significant decrease in GLP1 response at 15 min. No significant changes were observed in leptin, GIP and ghrelin after juice intake. Conclusions: In healthy individuals, a single acute consumption of fibre-enriched orange juice has short-term beneficial effects on postprandial glycaemia, circulating insulin levels and satiety through GLP1 secretion.
Vitamin B12 Deficiency Is Prevalent Among Czech Vegans Who Do Not Use Vitamin B12 Supplements
Authors: Selinger Eliška , Kühn Tilman , Procházková Magdalena , Anděl Michal , Gojda Jan
Published: December 2019

As not much is known about the prevalence and predictors of nutritional deficiencies among vegans in the Czech Republic, we evaluated whether supplement use and duration of adherence to the vegan diet are associated with the risk of cobalamin and iron deficiencies. Associations between self-reported supplementation and duration of vegan diet with biomarkers of cobalamin (serum cobalamin, holotranscobalamin, homocysteine, folate) and iron status (serum ferritin, iron binding capacity, transferrin and saturation of transferrin) were assessed by cross-sectional analyses of medical data from a clinical nutrition center. Data from 151 (72 females) adult vegans (age 18–67 years), who were free of major chronic diseases and 85 (40 females) healthy non-vegans (age 21–47 years) were analyzed. Overall, vegans had significantly lower cobalamin, hemoglobin and ferritin levels, but higher folate and MCV values compared to non-vegans. Vegans not using cobalamin supplements were at higher risk of low plasma cobalamin than regularly supplementing vegans (OR: 4.41, 95% CI 1.2–16.16 for cobalamin, OR: 19.18, 95% CI 1.02–359.42 for holotranscobalamin), whereas no significant differences in cobalamin status related to duration of the vegan diet were observed. Regularly supplementing vegans had similar levels of cobalamin/holotranscobalamin as non-vegans. Despite lower ferritin and hemoglobin levels, there was no indication of a higher risk of iron-deficiency among vegans. To conclude cobalamin deficiency risk depends on supplementation status and not on the duration of an exclusive vegan diet, which underlines the need to integrate cobalamin status monitoring and counselling on supplement use in routine clinical care in the Czech Republic.
Erratum: Dietary Patterns and Cardiovascular Risk Factors in Spanish Adolescents: A Cross-Sectional Analysis of the SI! Program for Health Promotion in Secondary Schools; Nutrients 2019, 11, 2297
Authors: Bodega Patricia , Fernández-Alvira Juan Miguel , Santos-Beneit Gloria , Cos-Gandoy Amaya de , Fernández-Jiménez Rodrigo , Moreno Luis Alberto , de Miguel Mercedes , Carral Vanesa , Orrit Xavier , Carvajal Isabel , Storniolo Carolina E. , Tresserra-Rimbau Anna , Doménech Mónica , Estruch Ramón , Lamuela-Raventós Rosa María , Fuster Valentín
Published: December 2019

The authors have requested that the following changes be made to their paper [...]
An Exploration of Common Greenhouse Gas Emissions by the Cyanobiont of the Azolla–Nostoc Symbiosis and Clues as to Nod Factors in Cyanobacteria
Authors: Gunawardana Dilantha
Published: December 2019

Azolla is a genus of aquatic ferns that engages in a unique symbiosis with a cyanobiont that is resistant to cultivation. Azolla spp. are earmarked as a possible candidate to mitigate greenhouse gases, in particular, carbon dioxide. That opinion is underlined here in this paper to show the broader impact of Azolla spp. on greenhouse gas mitigation by revealing the enzyme catalogue in the Nostoc cyanobiont to be a poor contributor to climate change. First, regarding carbon assimilation, it was inferred that the carboxylation activity of the Rubisco enzyme of Azolla plants is able to quench carbon dioxide on par with other C3 plants and fellow aquatic free-floating macrophytes, with the cyanobiont contributing on average ~18% of the carboxylation load. Additionally, the author demonstrates here, using bioinformatics and past literature, that the Nostoc cyanobiont of Azolla does not contain nitric oxide reductase, a key enzyme that emanates nitrous oxide. In fact, all Nostoc species, both symbiotic and nonsymbiotic, are deficient in nitric oxide reductases. Furthermore, the Azolla cyanobiont is negative for methanogenic enzymes that use coenzyme conjugates to emit methane. With the absence of nitrous oxide and methane release, and the potential ability to convert ambient nitrous oxide into nitrogen gas, it is safe to say that the Azolla cyanobiont has a myriad of features that are poor contributors to climate change, which on top of carbon dioxide quenching by the Calvin cycle in Azolla plants, makes it an efficient holistic candidate to be developed as a force for climate change mitigation, especially in irrigated urea-fed rice fields. The author also shows that Nostoc cyanobionts are theoretically capable of Nod factor synthesis, similar to Rhizobia and some Frankia species, which is a new horizon to explore in the future.
Seed Pretreatment and Foliar Application of Proline Regulate Morphological, Physio-Biochemical Processes and Activity of Antioxidant Enzymes in Plants of Two Cultivars of Quinoa (Chenopodium quinoa Willd.)
Authors: Yaqoob Hira , Akram Nudrat A. , Iftikhar Samrah , Ashraf Muhammad , Khalid Noman , Sadiq Muhammad , Alyemeni Mohammed Nasser , Wijaya Leonard , Ahmad Parvaiz
Published: December 2019

In the current study, the effects of exogenously applied proline (25 and 50 mM) and low-temperature treatment were examined on the physiochemical parameters in the plants of two cultivars (V1 and V2) of quinoa (Chenopodium quinoa Willd.). The seeds were also exposed to chilling stress at 4 °C before sowing. Plants raised from the seeds treated with low temperature showed reduced plant growth and contents of chlorophyll and carotenoids, but they had significantly increased contents of malondialdehyde, proline, ascorbic acid, total free amino acids, total soluble sugars, and total phenolics, as well as the activity of the peroxidase (POD) enzyme. Cold stress applied to seeds remained almost ineffective in terms of bringing about changes in plant root, hydrogen peroxide, glycine betaine and activities of superoxide dismutase (SOD), and catalase (CAT) enzymes. The exogenous application of proline significantly increased plant growth, the contents of chlorophyll, carotenoids, proline, ascorbic acid, total free amino acids, phenolics, and total soluble sugars, as well as the activities of SOD, POD, and CAT, but it decreased malondialdehyde content. Overall, foliar application of proline was better than the seed treatment in improving root dry weight, root length, chlorophyll a, carotenoids, glycine betaine, ascorbic acid and superoxide dismutase activity, whereas seed pre-treatment with proline was effective in improving shoot dry weight, shoot length, hydrogen peroxide, malondialdehyde, and peroxidase activity in both quinoa cultivars.
Stretching Wormlike Chains in Narrow Tubes of Arbitrary Cross-Sections
Authors: Li Ming , Wang Jizeng
Published: December 2019

We considered the stretching of semiflexible polymer chains confined in narrow tubes with arbitrary cross-sections. Based on the wormlike chain model and technique of normal mode decomposition in statistical physics, we derived a compact analytical expression on the force-confinement-extension relation of the chains. This single formula was generalized to be valid for tube confinements with arbitrary cross-sections. In addition, we extended the generalized bead-rod model for Brownian dynamics simulations of confined polymer chains subjected to force stretching, so that the confinement effects to the chains applied by the tubes with arbitrary cross-sections can be quantitatively taken into account through numerical simulations. Extensive simulation examples on the wormlike chains confined in tubes of various shapes quantitatively justified the theoretically derived generalized formula on the force-confinement-extension relation of the chains.
A Novel Hybrid Optimization Scheme on Connectivity Restoration Processes for Large Scale Industrial Wireless Sensor and Actuator Networks
Authors: Zhang Ying , Zhang Zheming , Zhang Bin
Published: December 2019

In the wireless sensor and actuator networks (WSANs) of industrial field monitoring, maintaining network connectivity with coverage perception plays a decisive role in many industrial process scenarios. The mobile actuator node is responsible for collecting data from the sensing nodes and performing diverse specific collaborative operation tasks. However, the failure of the nodes usually causes coverage vulnerability and partition of the network. Urgent and time-sensitive applications expect a minimum coverage loss to complete an instant connectivity restoration. This paper presents a hybrid coverage perception-based connectivity restoration algorithm, which is designed to restore network connectivity with minimal coverage area loss. The algorithm uses a backup node, which is selected nearby the critical node, to ensure a timely restoration when the critical node encounters failure. In the process of backup node migration, the optimal destination will be reselected to maintain the best network coverage after network connectivity recovery. The effectiveness of the proposed algorithm was verified by some simulation experiments.