bluuebunny/crossref_metadata_2025 huggingface dataset:
Episiotomy Practice and Its Associated Factors in Africa: A Systematic Review and Meta-Analysis
Authors: Woldegeorgis Beshada Zerfu , Obsa Mohammed Suleiman , Tolu Lemi Belay , Bogino Efa Ambaw , Boda Tesfalem Israel , Alemu Henok Berhanu
Published: June 2022

Background

Episiotomy, a surgical procedure that enlarges the vaginal opening during childbirth, was common practice until the early 2000s. Other sources, including the World Health Organization (WHO), advocate for the selective use of episiotomy. Episiotomy rates, on the other hand, have remained high in developing countries, while declining in developed countries. As a result, the current study sought to determine the overall prevalence of episiotomy in Africa as well as the risk factors associated with its practice.

Methods

Articles were searched in international electronic databases. A standardized Microsoft Excel spreadsheet and STATA software version 14 were used for data extraction and analysis, respectively. The Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) checklist was used to write this report. A random-effects meta-analysis model was used to determine the pooled prevalence of episiotomy. A heterogeneity test was conducted using I-Squared (I2) statistics. Egger's test and funnel plots were conducted to detect publication bias. Subgroup analysis was also conducted. Association was expressed through a pooled odds ratio (OR) with a 95% Confidence Interval (CI).

Result

A total of 21 studies with 40,831 participants were included in the systematic review and meta-analysis. The pooled prevalence of episiotomy practice was 41.7% [95% CI (36.0–47.4), I2 = 99.3%, P < 0.001). Primiparity [OR: 6.796 (95% CI (4.862–9.498)), P < 0.001, I2: 95.1%], medical doctors- assisted delivery [OR: 3.675 (95% CI (2.034–6.640)), P < 0.001, I2: 72.6%], prolonged second stage of labor [OR: 5.539 (95% CI (4.252–7.199)), P < 0.001, I2: 0.0%], using oxytocin [OR: 4.207 (95% CI (3.100–5.709)), P < 0.001, I2: 0.0%], instrument -assisted vaginal delivery [OR: 5.578 (95% CI (4.285–7.260)), P < 0.001, I2: 65.1%], and macrosomia [OR: 5.32 (95% CI (2.738–10.339)), P < 0.001, I2: 95.1%] were factors associated with episiotomy practice.

Conclusion

In this review, the prevalence of episiotomy among African parturients was high. A selective episiotomy practice should be implemented to reduce the high episiotomy rates.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021293382, identifier: CRD42021293382.
Phylogenomics and Spatiotemporal Dynamics of Bovine Leukemia Virus Focusing on Asian Native Cattle: Insights Into the Early Origin and Global Dissemination
Authors: Nishikaku Kohei , Yonezawa Takahiro , Nishibori Masahide , Harada Masashi , Kawaguchi Fuki , Sasazaki Shinji , Torii Yasushi , Imakawa Kazuhiko , Kawai Kuniko , Liu Jianquan , Mannen Hideyuki , Kobayashi Tomoko
Published: June 2022

Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis, is currently one of the most important pathogens affecting the cattle industry worldwide. Determining where and in which host it originated, and how it dispersed across continents will provide valuable insights into its historical emergence as the cattle pathogen. Various species in the Bos genus were domesticated in Asia, where they also diversified. As native cattle (taurine cattle, zebu cattle, yak, and water buffalo) are indigenous and adapted to local environments, we hypothesized that Asian native cattle could have harbored BLV and, therefore, that they were important for virus emergence, maintenance, and spread. In this study, phylogeographic and ancestral trait analyses—including sequences obtained from Asian native cattle—were used to reconstruct the evolutionary history of BLV. It was shown that, since its probable emergence in Asia, the virus spread to South America and Europe via international trade of live cattle. It was inferred that zebu cattle were the hosts for the early origin of BLV, while taurine cattle played the significant role in the transmission worldwide. In addition, the results of positive selection analysis indicate that yak had a substantially minor role in the transmission of this virus. In this study, endogenous deltaretrovirus sequences in bats, collected in Asian countries, were also analyzed on whether these sequences were present in the bat genome. Endogenous deltaretrovirus sequences were detected from bat species endemic to specific regions and geographically isolated for a long time. Endogenous deltaretrovirus sequences from these geographically isolated species represent ancient exogenous deltaretroviruses distributions. The phylogenetic analysis revealed that these newly obtained endogenous deltaretrovirus sequences were closely related to those of BLV from Asian native cattle, indicating that BLV-related ancient deltaretroviruses circulated in Asia long before the emergence of BLV. Together, our analyses provide evidence for origin and spatiotemporal dynamics of BLV.
Spatial Patterns and Composition Traits of Soil Microbial Nitrogen-Metabolism Genes in the Robinia pseudoacacia Forests at a Regional Scale
Authors: Ku Yongli , Lei Yuting , Han Xiaoting , Peng Jieying , Zhu Ying , Zhao Zhong
Published: June 2022

Microbial-driven processes related to the nitrogen-metabolism (N-metabolism) in soil are critical for ecosystem functioning and stability. There are spatial patterns of microbial-mediated nitrogen processes, but we still lack an overview of the soil N-metabolism genes of single nitrogen-fixing tree species pure forests at a regional scale. Here, we investigated the spatial variation and drivers of microbial N-metabolism genes in the rhizosphere soil of Robinia pseudoacacia on the Loess Plateau by metagenomic technology. We found that the distance-decay of soil N functional gene similarities in Robinia pseudoacacia forests on the Loess Plateau spanning a geographic distance of 230 km was significant (p < 0.001). The gene composition and co-occurrence patterns in the process of soil microbial N-metabolism were very different, and they were mainly driven by soil pH and MAP (mean annual precipitation). The proportion of positive links and edges co-occurrence networks between N functional genes increased with increasing pH, suggesting that increasing pH promoted connections between functional genes. The relative frequencies of N-metabolism pathways were consistent on the Loess Plateau, the abundance of ammonia assimilation pathway was highest, and the abundance of the nitrogen fixation pathway was the lowest; only the abundance of the nitrogen fixation pathway was not significantly different. The bacterial and archaeal communities involved in soil nitrogen metabolism were significantly different. Structural equation modeling showed that decreases in soil pH and MAP mainly affected the increase in nitrogen functional gene abundance through an increase in the diversity of N-metabolism microorganisms. In conclusion, this study provides a baseline for biogeographic studies of soil microbe functional genes.
Detection of Pseudomonas aeruginosa Serogroup G Using Real-Time PCR for Novel Target Genes Identified Through Comparative Genomics
Authors: Wang Chufang , Ye Qinghua , Ding Yu , Zhang Jumei , Gu Qihui , Pang Rui , Zhao Hui , Wang Juan , Wu Qingping
Published: June 2022

Accurate serotyping is essential for effective infection control. Pseudomonas aeruginosa serogroup G is one of the most common serogroups found in water. Conventional serotyping methods are not standardized and have several shortcomings. Therefore, a robust method for rapidly identifying P. aeruginosa serotypes is required. This study established a real-time PCR method for identifying P. aeruginosa serogroup G strains using novel target gene primers based on comparative genomic analysis. A total of 343 genome sequences, including 16 P. aeruginosa serogroups and 67 other species, were analyzed. Target genes identified were amplified using real-time PCR for detecting P. aeruginosa serogroup G strains. Eight serogroup G genes, PA59_01276, PA59_01887, PA59_01888, PA59_01891, PA59_01894, PA59_04268, PA59_01892, and PA59_01896, were analyzed to determine specific targets. A real-time fluorescence quantitative PCR method, based on the novel target PA59_01276, was established to detect and identify serogroup G strains. The specificity of this method was confirmed using P. aeruginosa serogroups and non-P. aeruginosa species. The sensitivity of this real-time PCR method was 4 × 102 CFU/mL, and it could differentiate and detect P. aeruginosa serogroup G in the range of 4.0 × 103–4.0 × 108 CFU/mL in artificially contaminated drinking water samples without enrichment. The sensitivity of these detection limits was higher by 1–3 folds compared to that of the previously reported PCR methods. In addition, the G serum group was accurately detected using this real-time PCR method without interference by high concentrations of artificially contaminated serum groups F and D. These results indicate that this method has high sensitivity and accuracy and is promising for identifying and rapidly detecting P. aeruginosa serogroup G in water samples. Moreover, this research will contribute to the development of effective vaccines and therapies for infections caused by multidrug-resistant P. aeruginosa.
Independent Component Analysis Identifies the Modulons Expanding the Transcriptional Regulatory Networks of Enterohemorrhagic Escherichia coli
Authors: Im Hanhyeok , Lee Ju-Hoon , Choi Sang Ho
Published: June 2022

The elucidation of the transcriptional regulatory networks (TRNs) of enterohemorrhagic Escherichia coli (EHEC) is critical to understand its pathogenesis and survival in the host. However, the analyses of current TRNs are still limited to comprehensively understand their target genes generally co-regulated under various conditions regardless of the genetic backgrounds. In this study, independent component analysis (ICA), a machine learning-based decomposition method, was used to decompose the large-scale transcriptome data of EHEC into the modulons, which contain the target genes of several TRNs. The locus of enterocyte effacement (LEE) and the Shiga toxin (Stx) modulons mainly consisted of the Ler regulon and the Stx prophage genes, respectively, confirming that ICA properly grouped the co-regulated major virulence genes of EHEC. Further investigation revealed that the LEE modulon contained the hypothetical Z0395 gene as a novel member of the Ler regulon, and the Stx modulon contained the thi and cus locus genes in addition to the Stx prophage genes. Correspondingly, the Stx prophage genes were also regulated by thiamine and copper ions known to control the thi and cus locus genes, respectively. The modulons effectively clustered the genes co-regulated regardless of the growth conditions and the genetic backgrounds of EHEC. The changed activities of the individual modulons successfully explained the differential expressions of the virulence and survival genes during the course of infection in bovines. Altogether, these results suggested that ICA of the large-scale transcriptome data can expand and enhance the current understanding of the TRNs of EHEC.
Mechanisms of Apoptotic Cell Death by Stainless Steel Nanoparticle Through Reactive Oxygen Species and Caspase-3 Activities on Human Liver Cells
Authors: Almutairi Bader , Ali Daoud , Yaseen Khadijah N. , Alothman Norah S. , Alyami Nouf , Almukhlafi Hanouf , Alakhtani Saad , Alarifi Saud
Published: June 2022

Background: Nanoparticles are widely used in pharmaceutical, agriculture, and food processing industries and in many other fields. However, the effect of stainless steel nanoparticles (SSNPs) remains unclear. So in this study, we evaluate the effect of SSNPs’ toxicity on human liver (CHANG and HuH-7) cell lines over 24 and 48 h.

Methods: We have analyzed the quality, shape, and size of SSNPs using x-ray diffraction (XRD), energy dispersive x-ray (EDX) scanning electron microscope (SEM), and transmission electron microscope (TEM). The cytotoxicity and cell growth were determined by using the MTT and wound healing tests. The oxidative stress parameters were determined by measuring ROS generation and antioxidant enzymes, such as glutathione (GSH) and superoxide dismutase (SOD), due to SSNP exposure on human liver cell lines over 24 and 48 h. The confirmation of the apoptotic effect of SSNPs on livers cells was determined by the Western blot analysis for the expression of apoptotic proteins, such as Bax, bcl2, and p53, and real-time PCR for the expression of apoptotic genes, such as Bax, bcl2, caspase-3, and p53.

Results: We have observed the dose- and time-dependent cytotoxicity and apoptosis of SSNPs on both cells. The results showed that SSNPs induced cell toxicity, inhibited cell growth, GSH, and increased generation of intracellular ROS and SOD levels at higher concentrations of exposure in both cells. SSNPs showed an apoptotic activity with upregulation of Bax, caspase-3, and p53 and downregulation of the bcl2 gene expression in CHANG and HuH-7 cell lines. Moreover, the immunoblotting assay confirmed the apoptotic activity of SSNPs in cells.

Conclusion: In conclusion, these findings demonstrated that SSNPs showed toxic effects on human liver cells via activating the caspase-3 activity and they induced more toxicity in HuH-7 cells than in CHANG cells.
Recent Technical Advances in Sample Preparation for Single-Particle Cryo-EM
Authors: Xu Yixin , Dang Shangyu
Published: June 2022

Cryo-sample preparation is a vital step in the process of obtaining high-resolution structures of macromolecules by using the single-particle cryo–electron microscopy (cryo-EM) method; however, cryo-sample preparation is commonly hampered by high uncertainty and low reproducibility. Specifically, the existence of air-water interfaces during the sample vitrification process could cause protein denaturation and aggregation, complex disassembly, adoption of preferred orientations, and other serious problems affecting the protein particles, thereby making it challenging to pursue high-resolution 3D reconstruction. Therefore, sample preparation has emerged as a critical research topic, and several new methods for application at various preparation stages have been proposed to overcome the aforementioned hurdles. Here, we summarize the methods developed for enhancing the quality of cryo-samples at distinct stages of sample preparation, and we offer insights for developing future strategies based on diverse viewpoints. We anticipate that cryo-sample preparation will no longer be a limiting step in the single-particle cryo-EM field as increasing numbers of methods are developed in the near future, which will ultimately benefit the entire research community.
Multifaceted Roles of Ferroptosis in Lung Diseases
Authors: Li Yi , Yang Ying , Yang Yongfeng
Published: June 2022

Ferroptosis is a distinct type of programmed cell death (PCD) that depends on iron and is characterized by the accumulation of intracellular iron, exhaustion of glutathione, deactivation of glutathione peroxidase, and promotion of lipid peroxidation. Recently, accumulated investigations have demonstrated that ferroptosis is strongly correlated with the initiation and development of many lung diseases. In this review, we summarized the contribution of ferroptosis to the pathologic process of lung diseases, namely, obstructive lung diseases (chronic obstructive pulmonary disease, asthma, and cystic fibrosis), interstitial lung diseases (pulmonary fibrosis of different causes), pulmonary diseases of vascular origin (ischemia-reperfusion injury and pulmonary hypertension), pulmonary infections (bacteria, viruses, and fungi), acute lung injury, acute respiratory distress syndrome, obstructive sleep apnea, pulmonary alveolar proteinosis, and lung cancer. We also discussed the therapeutic potential of targeting ferroptosis for these lung diseases.
Hearing Impairment With Cognitive Decline Increases All-Cause Mortality Risk in Chinese Adults Aged 65 Years or Older: A Population-Based Longitudinal Study
Authors: Wang Jun , Liu Dan , Tian E. , Guo Zhao-Qi , Chen Jing-Yu , Kong Wei-Jia , Zhang Su-Lin
Published: June 2022

Background

Hearing impairment (HI), a highly prevalent sensory impairment affecting older adults, is a risk factor for cognitive decline. However, few studies examined the association between HI and all-cause mortality, and the role of different cognitive states on this relationship in Chinese older adults is poorly understood.

Methods

A total of 10,744 Chinese older adults aged 65 years or older were included in the 2011/2012 and 2014 cohorts from the Chinese Longitudinal Healthy Longevity Survey (CLHLS), with the longest follow-up period lasting for up to 8 years. The presence of HI was identified by using a dichotomized metric of self-reported hearing status. All-cause mortality data were ascertained from interviews with family members or relatives of the participants. Cognitive function was evaluated by employing the modified Mini-Mental State Examination (MMSE), which consisted of seven subdomains (orientation, naming foods, registration, attention and calculation, copy figure, delayed recall, and speech and language). Kaplan–Meier survival curves were constructed to evaluate the different hearing states on overall survival. The risk of mortality over the follow-up period was estimated by using Cox proportional hazard ratios (HRs) models.

Results

A conspicuous probability was revealed in the survival relationship between hearing status and all-cause mortality for the total population (p < 0.001). Participants with HI had a higher risk of all-cause mortality (HR = 2.29, 95% CI: 2.16, 2.42), as compared with their counterparts without HI. The association was robust upon fully adjustment for potential confounders (HR = 1.07, 95% CI: 1.00, 1.14). Compared to HI participants with no cognitive impairment, HI patients with cognitive impairment had a higher mortality risk (HR = 2.31, 95% CI: 2.13, 2.51). Impairment in the subdomains of cognitive function were independently associated with elevated mortality risk in the participants with HI, with an HR ranging from 1.28 (copy figure) to 1.46 (speech and language).

Conclusions

Cognitive decline was common in individuals with HI, and those with HI and cognitive impairment further increased mortality risk. Our findings prompt a call for actions to improve the hearing status and cognitive function of older people to minimize health risks and improve longevity.
Evaluation of Feature Selection for Alzheimer’s Disease Diagnosis
Authors: Gu Feng , Ma Songhua , Wang Xiude , Zhao Jian , Yu Ying , Song Xinjian
Published: June 2022

Accurate recognition of patients with Alzheimer’s disease (AD) or mild cognitive impairment (MCI) is important for the subsequent treatment and rehabilitation. Recently, with the fast development of artificial intelligence (AI), AI-assisted diagnosis has been widely used. Feature selection as a key component is very important in AI-assisted diagnosis. So far, many feature selection methods have been developed. However, few studies consider the stability of a feature selection method. Therefore, in this study, we introduce a frequency-based criterion to evaluate the stability of feature selection and design a pipeline to select feature selection methods considering both stability and discriminability. There are two main contributions of this study: (1) It designs a bootstrap sampling-based workflow to simulate real-world scenario of feature selection. (2) It develops a decision graph to determine the optimal combination of supervised and unsupervised feature selection both considering feature stability and discriminability. Experimental results on the ADNI dataset have demonstrated the feasibility of our method.
The Association Between Grip Strength and Depression Among Adults Aged 60 Years and Older: A Large-Scaled Population-Based Study From the Longitudinal Aging Study in India
Authors: Wang Jinbao , Zhou Xianghong , Qiu Shi , Deng Linghui , Li Jiakun , Yang Lu , Wei Qiang , Dong Birong
Published: June 2022

Background

The association between grip strength and depression in elderly individuals in low- and middle-income countries (LMICs) has rarely been studied. This study aims to explore the relevance of grip strength and depression in the elderly population using data from a national large-scale population.

Methods

This study was conducted using data from seniors over 60 years old in wave 1 of the Longitudinal Aging Study in India (LASI). Grip strength is the maximum of three measurements by the dynamometer. Depression symptoms were assessed using 10 items on the Center for Epidemiologic Studies Depression Scale (CESD-10) with a 10-point boundary. Multivariate linear regression analysis, non-linear analysis, subgroup analysis, interaction tests and sensitivity analysis were performed.

Results

There were 27,343 participants in this study, including 19,861 participants with low grip strength and 7,482 participants with normal grip strength. The results revealed that grip strength and depression were negatively correlated in elderly individuals after adequate adjustment for confounding factors [odds ratio (OR) = 1.237, 95% confidence interval (CI) 1.172–1.305, p < 0.00001]. The results remained stable after adjusting for all confounding factors (OR = 1.090, 95% CI 1.030–1.155, p = 0.00307). Regression analysis showed that physical activity (PA), comorbidities and cognition may have an impact on the correlation between grip strength and depression symptoms. Smooth curve fit suggested that grip strength and depressive symptoms were linearly related. The interaction test results of gender in the relationship between grip strength and depression were significant (p for interaction < 0.05).

Conclusion

Grip strength and depression were negatively correlated in older Indians, and larger prospective studies are needed in the future to determine this association.
Discriminant Subspace Low-Rank Representation Algorithm for Electroencephalography-Based Alzheimer’s Disease Recognition
Authors: Tang Tusheng , Li Hui , Zhou Guohua , Gu Xiaoqing , Xue Jing
Published: June 2022

Alzheimer’s disease (AD) is a chronic progressive neurodegenerative disease that often occurs in the elderly. Electroencephalography (EEG) signals have a strong correlation with neuropsychological test results and brain structural changes. It has become an effective aid in the early diagnosis of AD by exploiting abnormal brain activity. Because the original EEG has the characteristics of weak amplitude, strong background noise and randomness, the research on intelligent AD recognition based on machine learning is still in the exploratory stage. This paper proposes the discriminant subspace low-rank representation (DSLRR) algorithm for EEG-based AD and mild cognitive impairment (MCI) recognition. The subspace learning and low-rank representation are flexibly integrated into a feature representation model. On the one hand, based on the low-rank representation, the graph discriminant embedding is introduced to constrain the representation coefficients, so that the robust representation coefficients can preserve the local manifold structure of the EEG data. On the other hand, the least squares regression, principle component analysis, and global graph embedding are introduced into the subspace learning, to make the model more discriminative. The objective function of DSLRR is solved by the inexact augmented Lagrange multiplier method. The experimental results show that the DSLRR algorithm has good classification performance, which is helpful for in-depth research on AD and MCI recognition.
Task-Dependent Effective Connectivity of the Reward Network During Food Cue-Reactivity: A Dynamic Causal Modeling Investigation
Authors: Ghobadi-Azbari Peyman , Mahdavifar Khayati Rasoul , Sangchooli Arshiya , Ekhtiari Hamed
Published: June 2022

Neural reactivity to food cues may play a central role in overeating and excess weight gain. Functional magnetic resonance imaging (fMRI) studies have implicated regions of the reward network in dysfunctional food cue-reactivity, but neural interactions underlying observed patterns of signal change remain poorly understood. Fifty overweight and obese participants with self-reported cue-induced food craving viewed food and neutral cues during fMRI scanning. Regions of the reward network with significantly greater food versus neutral cue-reactivity were used to specify plausible models of task-related neural interactions underlying the observed blood oxygenation level-dependent (BOLD) signal, and a bi-hemispheric winning model was identified in a dynamic causal modeling (DCM) framework. Neuro-behavioral correlations are investigated with group factor analysis (GFA) and Pearson’s correlation tests. The ventral tegmental area (VTA), amygdalae, and orbitofrontal cortices (OFC) showed significant food cue-reactivity. DCM suggests these activations are produced by largely reciprocal dynamic signaling between these regions, with food cues causing regional disinhibition and an apparent shifting of activity to the right amygdala. Intrinsic self-inhibition in the VTA and right amygdala is negatively correlated with measures of food craving and hunger and right-amygdalar disinhibition by food cues is associated with the intensity of cue-induced food craving, but no robust cross-unit latent factors were identified between the neural group and behavioral or demographic variable groups. Our results suggest a rich array of dynamic signals drive reward network cue-reactivity, with the amygdalae mediating much of the dynamic signaling between the VTA and OFCs. Neuro-behavioral correlations suggest particularly crucial roles for the VTA, right amygdala, and the right OFC-amygdala connection but the more robust GFA identified no cross-unit factors, so these correlations should be interpreted with caution. This investigation provides novel insights into dynamic circuit mechanisms with etiologic relevance to obesity, suggesting pathways in biomarker development and intervention.
The Potential for EBV Vaccines to Prevent Multiple Sclerosis
Authors: Maple Peter A. , Ascherio Alberto , Cohen Jeffrey I. , Cutter Gary , Giovannoni Gavin , Shannon-Lowe Claire , Tanasescu Radu , Gran Bruno
Published: June 2022

There is increasing evidence suggesting that Epstein-Barr virus infection is a causative factor of multiple sclerosis (MS). Epstein-Barr virus (EBV) is a human herpesvirus, Human Gammaherpesvirus 4. EBV infection shows two peaks: firstly, during early childhood and, secondly during the teenage years. Approximately, 90–95% of adults have been infected with EBV and for many this will have been a subclinical event. EBV infection can be associated with significant morbidity and mortality; for example, primary infection in older children or adults is the leading cause of infectious mononucleosis (IM). A disrupted immune response either iatrogenically induced or through genetic defects can result in lymphoproliferative disease. Finally, EBV is oncogenic and is associated with several malignancies. For these reasons, vaccination to prevent the damaging aspects of EBV infection is an attractive intervention. No EBV vaccines have been licensed and the prophylactic vaccine furthest along in clinical trials contains the major virus glycoprotein gp350. In a phase 2 study, the vaccine reduced the rate of IM by 78% but did not prevent EBV infection. An EBV vaccine to prevent IM in adolescence or young adulthood is the most likely population-based vaccine strategy to be tested and adopted. National registry studies will need to be done to track the incidence of MS in EBV-vaccinated and unvaccinated people to see an effect of the vaccine on MS. Assessment of vaccine efficacy with MS being a delayed consequence of EBV infection with the average age of onset being approximately 30 years of age represents multiple challenges.
Case Report: Genetic Creutzfeldt–Jakob Disease With a G114V Mutation and One Octapeptide Repeat Deletion as a Mimic of Frontotemporal Dementia
Authors: Lin Xue , Xu Yichen , Zhen Zhen , Xiao Kang , Chen Xu , Yang Jigang , Guan Hongzhi , Shi Qi , Dong Xiaoping , Wang Jiawei , Guo Yanjun
Published: June 2022

Genetic Creutzfeldt–Jakob disease (gCJD) characterized by mutations in the prion protein (PrP) gene (PRNP) contributes to approximately 10–15% of the overall human prion diseases. Here, we report a rare mutation in the PRNP gene in a Han-Chinese family. A 36-year-old man initiated with anxiety and depression followed by progressive dementia, cogwheel-like rigidity combined with tremors, and he was diagnosed with frontotemporal lobar dementia in the first 2 years. The disease progression was relatively slow, and the patient developed into akinetic mutism in 4 years. To characterize the disease, following the pedigree studies, neuropsychological examination, neuroimaging studies, real-time quaking-induced conversion (RT-QuIC) examination, and so on were conducted. We eventually identified a rare mutation of G114V combined with one octapeptide repeats deletion (1-ORPD) in the PrP in the patient by DNA sequencing. In addition, the same mutation and deletion were subsequently identified in the patient's mother without any syndromes. His maternal grandmother had a late onset of the disease in her 60s. Given that 1-OPRD has never been reported in human prion disease before, our first report that both G114V mutation and 1-OPRD appear in the family would forward our understanding of the etiological mechanisms of the gCJD.
Case Report: Can Ruptured Aneurysms in the Hypoplastic and Plexiform Posterior Inferior Cerebellar Arteries Be Safely Occluded?
Authors: Hou Kun , Yu Jinlu
Published: June 2022

Aneurysms arising from the posterior inferior cerebellar artery (PICA) are not rare and may originate from the proximal or peripheral segment of the PICA. However, when the affected PICA is hypoplastic and plexiform, it is difficult to occlude the aneurysm without sacrificing the parent vessel, the PICA. This type of aneurysm is rare, and whether it is safe to occlude the aneurysm and the parent artery, in cases of a ruptured aneurysm of the hypoplastic and plexiform PICA, has not been adequately studied and is still open to debate. In this report, two patients with ruptured aneurysms in the hypoplastic and plexiform PICA were presented. Both patients were admitted to our hospital for subarachnoid hemorrhage. After team discussions between the neurosurgeons and neurointerventionalists, the aneurysm and parent PICA had to be occluded via endovascular treatment under general anesthesia. One of the patients developed postprocedural brainstem infarction and exhibited favorable recovery. The other patient died of pulmonary infection, although improvement in the postoperative state was observed. Although rare, aneurysms can originate from the hypoplastic and plexiform PICA. Occluding the aneurysm and hypoplastic parent PICA via endovascular treatment might be a reasonable option.
Error-Related Potentials in Reinforcement Learning-Based Brain-Machine Interfaces
Authors: Xavier Fidêncio Aline , Klaes Christian , Iossifidis Ioannis
Published: June 2022

The human brain has been an object of extensive investigation in different fields. While several studies have focused on understanding the neural correlates of error processing, advances in brain-machine interface systems using non-invasive techniques further enabled the use of the measured signals in different applications. The possibility of detecting these error-related potentials (ErrPs) under different experimental setups on a single-trial basis has further increased interest in their integration in closed-loop settings to improve system performance, for example, by performing error correction. Fewer works have, however, aimed at reducing future mistakes or learning. We present a review focused on the current literature using non-invasive systems that have combined the ErrPs information specifically in a reinforcement learning framework to go beyond error correction and have used these signals for learning.
The Effectiveness of Targeted Electrical Stimulation via Cochlear Implant on Tinnitus-Perceived Loudness
Authors: Di Nardo Walter , Di Cesare Tiziana , Tizio Angelo , Paludetti Gaetano , Fetoni Anna Rita
Published: June 2022

Introduction

The cause of tinnitus improvement in cochlear implant (CI) users is not understood. On the basis that a spatially limited dysfunction in the auditory pathway could cause tinnitus, we used single-channel stimulation to evaluate any variation of tinnitus-perceived loudness and identify the cochlear regions involved.

Materials and Methods

It was an observational prospective case-crossover study. After the first mapping, 21 adults with unilateral CI and chronic tinnitus expressed their tinnitus loudness based on the Visual Analog Scale (VAS) score (0–10) at baseline (L0), during a 10 s single-channel stimulation with C-level of electric current (L1) and 30 min after CI activation (L2). Tinnitus reduction [RT = (L0 – L1) × 100/L0] > 50% was considered significant. VAS outcomes were compared between baseline (L0) and (each) single-channel stimulation (L1) to find the channel with the greatest RT (suppressive channel-SC), whose frequency range revealed the cochlear region involved. Seven patients with asymmetric hearing loss underwent the pitch-matching test to identify the actual frequency evoked by the SC. We compared selective (L1) and non-selective (L2) intracochlear stimulation using paired t-test. Preoperative Tinnitus Handicap Inventory (THI) score was compared with those at 1, 6, and 12 months with paired t-tests to evaluate long-term tinnitus perception.

Results

We observed a significant reduction of tinnitus loudness during the experimental procedure [L0 (6.4 ± 2.4) vs. L1 (1.7 ± 2.7), p = 0.003]. A total of 15/21 patients (71.4%) had a significant (RT > 50%) and selective improvement, reporting a mean L1 of 0.4 ± 2.0 (p = 0.0001). In 10/15 (66.6%) patients, the SC was in the apical turn, within 1,000 Hz; in 5/15 patients (33.4%) within 4,000 Hz. The cochlear region 125–313 Hz was the most affected by tinnitus improvement (p = 0.0074). Targeted stimulation was more effective than non-selective stimulation [L1 vs. L2 (4.3 ± 2.5), p = 0.0022]. In 3/7 patients, the perceived pitch did not fall within the SC frequency ranges. All patients with selective attenuation described tinnitus as monotone. Patients with non-selective attenuation had polyphonic tinnitus and better THI results after 1 year.

Conclusion

Targeted intracochlear electrical stimulation improved chronic tinnitus perception, especially in monotone tinnitus, and the apical region was mainly involved. Our results provide new insights into the pathophysiological mechanisms of tinnitus and targets for innovative therapeutic strategies.
Larval Zebrafish as a Model for Mechanistic Discovery in Mental Health
Authors: Tan Jazlynn Xiu Min , Ang Ryan Jun Wen , Wee Caroline Lei
Published: June 2022

Animal models are essential for the discovery of mechanisms and treatments for neuropsychiatric disorders. However, complex mental health disorders such as depression and anxiety are difficult to fully recapitulate in these models. Borrowing from the field of psychiatric genetics, we reiterate the framework of ‘endophenotypes’ – biological or behavioral markers with cellular, molecular or genetic underpinnings – to reduce complex disorders into measurable behaviors that can be compared across organisms. Zebrafish are popular disease models due to the conserved genetic, physiological and anatomical pathways between zebrafish and humans. Adult zebrafish, which display more sophisticated behaviors and cognition, have long been used to model psychiatric disorders. However, larvae (up to 1 month old) are more numerous and also optically transparent, and hence are particularly suited for high-throughput screening and brain-wide neural circuit imaging. A number of behavioral assays have been developed to quantify neuropsychiatric phenomena in larval zebrafish. Here, we will review these assays and the current knowledge regarding the underlying mechanisms of their behavioral readouts. We will also discuss the existing evidence linking larval zebrafish behavior to specific human behavioral traits and how the endophenotype framework can be applied. Importantly, many of the endophenotypes we review do not solely define a diseased state but could manifest as a spectrum across the general population. As such, we make the case for larval zebrafish as a promising model for extending our understanding of population mental health, and for identifying novel therapeutics and interventions with broad impact.
Exogenous Application of Glycine Betaine Maintains Bioactive Compounds, Antioxidant Activity, and Physicochemical Attributes of Blood Orange Fruit During Prolonged Cold Storage
Authors: Habibi Fariborz , Valero Daniel , Serrano María , Guillén Fabián
Published: June 2022

Exogenous application of glycine betaine (GB) was evaluated on bioactive compounds, antioxidant activity, and physicochemical attributes of blood orange fruit cv. Moro at 3°C for 90 days. Vacuum infiltration (30 kPa) of GB was applied at 15 and 30 mM for 8 min. Parameters were measured after 1, 30, 60, and 90 days of storage plus 2 days at 20°C to simulate the shelf-life period. GB treatments significantly reduced weight and firmness losses in “Moro” blood orange fruit during cold storage. GB treatment maintained a higher concentration of organic acids (citric, malic, succinic, and oxalic acids) and sugars (sucrose, glucose, and fructose), especially for the higher GB doses (30 mM). During storage, GB treatments enhanced total anthocyanin concentration, total phenolic content, and total antioxidant activity. With respect to enzyme activities, the application of exogenous GB showed increases in catalase (CAT), ascorbate peroxidase, superoxide dismutase, phenylalanine ammonia-lyase, while suppressing the polyphenol oxidase activity. Overall, the most effective treatment was 30 mM GB leading to maintaining bioactive compounds, antioxidant activity, and quality in “Moro” blood orange fruit during long-term storage. The positive results would permit the use of GB as a postharvest tool to maintain the quality attributes of blood orange fruit.
Association of Tibetan Habitual Food and Metabolic Syndrome Among Tibetan People in China: A Cross-Sectional Study
Authors: Li Kehan , Zhang Qiang , Cai Hui , He Ruifeng , Nima Qucuo , Li Yajie , Suolang Deji , Cidan Zhuoga , Wangqing Pingcuo , Zhao Xing , Li Jingzhong , Liu Qiaolan
Published: June 2022

Background

The association between habitual food intake in Tibet and metabolic syndrome (MetS) is largely unclear.

Objective

To examine the association between Tibetan habitual food intake and MetS among Tibetan adults.

Methods

A population-based cross-sectional study, named the China Multi-Ethnic Cohort (CMEC) study, was conducted between 2018 and 2019. We used data from all Tibetans in the CMEC in the current study. The participants, 1,954 men and 3,060 women aged 18–79 years, were from Lhasa, Tibet Autonomous Region, Tibet. The habitual dietary intake was assessed using a food frequency questionnaire (FFQ). MetS was defined according to ATP III guidelines. Multivariate logistic regression was used to estimate the association between five Tibetan habitual foods and MetS.

Results

Tsampa, butter tea, and Qing cha intake were associated with reduced prevalence of MetS. Compared with the lowest quartile of each food, odds ratios (ORs) and their 95% confidence intervals (95% CIs) of medium and high Tsampa intake were 0.59 (0.41–0.85) and 0.53 (0.36–0.77), ORs (95% CIs) of butter tea were 0.67 (0.52–0.88) and 0.61 (0.46–0.81), and Qing cha were 0.85 (0.71–1.03) and 0.75 (0.60–0.93), respectively. When exploring the joint effects of these three foods on MetS, the adjusted ORs and their 95% CIs were 0.65 (0.49–0.87) for the middle intake group and 0.59 (0.42–0.83) for the high intake group as compared with the never/rarely group (p = 0.022 for trend). Associations of MetS with Tibetan noodles and raw beef were not observed.

Conclusion

Tsampa, butter tea, and Qing cha were negatively associated with MetS. The recommendation of increasing the intake of these foods may be beneficial for MetS prevention.
Westernized and Diverse Dietary Patterns Are Associated With Overweight-Obesity and Abdominal Obesity in Mexican Adult Men
Authors: Rodríguez-Ramírez Sonia , Martinez-Tapia Brenda , González-Castell Dinorah , Cuevas-Nasu Lucía , Shamah-Levy Teresa
Published: June 2022

Introduction

The prevalence of overweight and obesity in Mexican adults is very high. To identify the dietary characteristics related with this disorder is necessary to design intervention. The objective was to analyze the association between dietary patterns and obesity in Mexican adults.

Materials and Methods

This is a cross-sectional study carried out in Mexican adults (20–59 years old) participating in the Halfway National Health and Nutrition Survey 2016. Participants (n = 5,735) were classified as having normal weight, overweight-obesity and by their abdominal circumference as having abdominal obesity or not. With information from a 7-day food frequency questionnaire, we used a K-means cluster analysis to derive dietary patterns and calculated a healthy diet indicator to evaluate quality. The association between dietary patterns and overweight-obesity and abdominal obesity was assessed with Poisson regression models adjusted by some characteristics.

Results

We identified a Rural pattern characterized by tortilla, legumes and egg consumption; a Diverse pattern, characterized by fruits, meat and poultry, vegetables, and dairy beverages, and desserts; and a Westernized pattern, characterized by sweetened non-dairy beverages, fast food, bakery and cookies, candies and salty snacks. In men, Westernized pattern was associated with overweight-obesity (PR = 1.11, 95% CI 0.97–1.27), and abdominal obesity (PR = 1.15, 95% CI 1.00–1.33), the Diverse pattern was associated with overweight-obesity (PR = 1.18, 95% CI 1.00–1.38), and abdominal obesity (PR = 1.27, 95% CI 1.07–1.50), compared with the Rural pattern. In women, these dietary patterns were not associated with obesity.

Discussion

Westernized and Diverse patterns are associated with overweight and obesity and abdominal obesity in men. Gender-specific recommendations and surveillance are necessary in the Mexican adult population.
Malnutrition and Poor Physical Function Are Associated With Higher Comorbidity Index in Hospitalized Older Adults
Authors: Amasene Maria , Medrano María , Echeverria Iñaki , Urquiza Miriam , Rodriguez-Larrad Ana , Diez Amaia , Labayen Idoia , Ariadna Besga-Basterra
Published: June 2022

Background

The Charlson Comorbidity Index (CCI) is the most widely used method to measure comorbidity and predict mortality. There is no evidence whether malnutrition and/or poor physical function are associated with higher CCI in hospitalized patients. Therefore, this study aimed to (i) analyze the association between the CCI with nutritional status and with physical function of hospitalized older adults and (ii) examine the individual and combined associations of nutritional status and physical function of older inpatients with comorbidity risk.

Methods

A total of 597 hospitalized older adults (84.3 ± 6.8 years, 50.3% women) were assessed for CCI, nutritional status (the Mini Nutritional Assessment-Short Form [MNA-SF]), and physical function (handgrip strength and the Short Physical Performance Battery [SPPB]).

Results

Better nutritional status (p < 0.05) and performance with handgrip strength and the SPPB were significantly associated with lower CCI scores among both men (p < 0.005) and women (p < 0.001). Patients with malnutrition or risk of malnutrition (OR: 2.165, 95% CI: 1.408–3.331, p < 0.001) as well as frailty (OR: 3.918, 95% CI: 2.326–6.600, p < 0.001) had significantly increased the risk for being at severe risk of comorbidity. Patients at risk of malnutrition or that are malnourished had higher CCI scores regardless of being fit or unfit according to handgrip strength (p for trend < 0.05), and patients classified as frail had higher CCI despite their nutritional status (p for trend < 0.001).

Conclusions

The current study reinforces the use of the MNA-SF and the SPPB in geriatric hospital patients as they might help to predict poor clinical outcomes and thus indirectly predict post-discharge mortality risk.
Obesity-Related Chronic Kidney Disease: Principal Mechanisms and New Approaches in Nutritional Management
Authors: Stasi Alessandra , Cosola Carmela , Caggiano Gianvito , Cimmarusti Maria Teresa , Palieri Rita , Acquaviva Paola Maria , Rana Gloria , Gesualdo Loreto
Published: June 2022

Obesity is the epidemic of our era and its incidence is supposed to increase by more than 30% by 2030. It is commonly defined as a chronic and metabolic disease with an excessive accumulation of body fat in relation to fat-free mass, both in terms of quantity and distribution at specific points on the body. The effects of obesity have an important impact on different clinical areas, particularly endocrinology, cardiology, and nephrology. Indeed, increased rates of obesity have been associated with increased risk of cardiovascular disease (CVD), cancer, type 2 diabetes (T2D), dyslipidemia, hypertension, renal diseases, and neurocognitive impairment. Obesity-related chronic kidney disease (CKD) has been ascribed to intrarenal fat accumulation along the proximal tubule, glomeruli, renal sinus, and around the kidney capsule, and to hemodynamic changes with hyperfiltration, albuminuria, and impaired glomerular filtration rate. In addition, hypertension, dyslipidemia, and diabetes, which arise as a consequence of overweight, contribute to amplifying renal dysfunction in both the native and transplanted kidney. Overall, several mechanisms are closely related to the onset and progression of CKD in the general population, including changes in renal hemodynamics, neurohumoral pathways, renal adiposity, local and systemic inflammation, dysbiosis of microbiota, insulin resistance, and fibrotic process. Unfortunately, there are no clinical practice guidelines for the management of patients with obesity-related CKD. Therefore, dietary management is based on the clinical practice guidelines for the nutritional care of adults with CKD, developed and published by the National Kidney Foundation, Kidney Disease Outcome Quality Initiative and common recommendations for the healthy population. Optimal nutritional management of these patients should follow the guidelines of the Mediterranean diet, which is known to be associated with a lower incidence of CVD and beneficial effects on chronic diseases such as diabetes, obesity, and cognitive health. Mediterranean-style diets are often unsuccessful in promoting efficient weight loss, especially in patients with altered glucose metabolism. For this purpose, this review also discusses the use of non-classical weight loss approaches in CKD, including intermittent fasting and ketogenic diet to contrast the onset and progression of obesity-related CKD.