bluuebunny/crossref_metadata_2025 huggingface dataset:
The MIR 2018 Exam: Psychometric Study and Comparison with the Previous Nine Years
Authors: Baladrón Jaime , Sánchez Lasheras Fernando , Romeo Ladrero José María , Villacampa Tomás , Curbelo José , Jiménez Fonseca Paula , García Guerrero Alberto
Published: November 2019

Background and Objectives: The aim of the present research is to study the questions used in the 2018 MIR exam (a test that allows access to specialized medical training in Spain), describe their psychometric properties, and evaluate their quality. Materials and Methods: This analysis is performed with the help of classical test theory (CTT) and item response theory (IRT). The answers given to the test questions by a total of 3868 physicians are analyzed. Results: According to CTT, the average difficulty index for all of the test questions was 0.629, which falls into the acceptable category. The average difficulty index with correction for random effects was 0.515, which corresponds to a value within the optimal range. The mean discrimination index was 0.277, which is in the good category, while the mean point biserial correlation coefficient, with a value of 0.275 fits in the regular category. The values of difficulty and discrimination calculated according to the model of two parameters of the IRT seem adequate with average values of −0.389 and 0.677. The Cronbach alpha score obtained for the overall test was 0.944. This value is considered as very good. Conclusions: A decrease was observed in the average values of discrimination in the last three calls, which may be related to the greater proportion of Spanish graduates that take the exam in the same year of finalization of their studies in Medicine.
Perfusion Index Analysis in Patients Presenting to the Emergency Department Due to Synthetic Cannabinoid Use
Authors: Yeniocak Selman
Published: November 2019

Background and Objectives: The perfusion index (PI) indicates the ratio of pulsatile blood flow in peripheral tissue to non-pulsatile blood flow. This study was performed to examine the blood perfusion status of tissues and organs of patients using synthetic cannabinoids (SCs). Materials and Methods: The records of patients aged 17 or over presenting to the adult emergency department due to SC use between 1 January 2016 and 31 December 2017 were examined in this single-center, retrospective, cross-sectional study. Examined factors included time from consumption of SC to presentation to the emergency department, as well as simultaneously determined systolic and diastolic blood pressures, heart rate (beats per min), Glasgow Coma Score (GCS), and PI values. Patients were divided into two groups, A and B, depending on the amount of time that had elapsed between SC consumption and presentation to the emergency department, and statistical data were compared. Results: The mean PI value in Group A was lower than that in Group B. Therefore, we concluded that peripheral tissue and organ blood perfusion is lower in the first 2 h following SC consumption than after 2 h. Systolic, diastolic, and mean arterial blood pressure and mean GCS values were also statistically significantly lower in Group A than in Group B. Conclusions: A decreased PI value may be an early sign of reduced-perfusion organ damage. PI is a practical and useful parameter in the early diagnosis of impaired organ perfusion and in monitoring tissue hypoxia leading to organ failure.
The Role of C21orf91 in Herpes Simplex Virus Keratitis
Authors: Danileviciene Vilija , Zemaitiene Reda , Gintauskiene Vilte Marija , Nedzelskiene Irena , Zaliuniene Dalia
Published: November 2019

Background and Objectives: This paper aims to describe the single nucleotide polymorphisms (SNPs) of C21orf91 rs1062202 and rs10446073 in patients with herpetic keratitis by evaluating corneal sub-basal nerves, as well as the density of Langerhans cells (LC) and endothelium cells (EC) during the acute phase of the disease. Materials and Methods: A prospective clinical study included 260 subjects: 70 with herpetic eye disease, 101 with previous history of herpes labialis—but no history of herpetic eye disease—and 89 with no history of any herpes simplex virus (HSV) diseases. All subjects underwent a complete ophthalmological examination including in vivo laser scanning confocal microscopy (LSCM) of the central cornea. C21orf91 rs1062202 and rs10446073 were genotyped using the real-time polymerase chain reaction (PCR) method with the Rotor-Gene Q real-time PCR quantification system. SNPs were determined using TaqMan genotyping assay, according to the manufacturer’s manual. Results: The C21orf91 rs10446073 genotype GT was more frequent in the HSV keratitis group, compared with healthy controls (20.0% vs. 7.9%), OR 2.929[1.11–7.716] (p < 0.05). The rs10446073 genotype TT was more frequent in healthy controls (12.4% vs. 1.4%), OR 22.0[2.344–260.48] (p < 0.05). The rs10446073 genotype GT increased the risk of EC density being less than 2551.5 cell/mm2, OR 2.852[1.248–6.515] (p < 0.05). None of the SNPs and their genotypes influenced the LC density and corneal sub-basal nerve parameters (p > 0.05). Conclusions: Our study reports a new association between herpetic keratitis and human gene C21orf91, with the rs10446073 genotype GT being more common in herpetic keratitis patients and increasing the risk for the disease by a factor of 2.9.
Synthesis of Zeolite Using Aluminum Dross and Waste LCD Glass Powder: A Waste to Waste Integration Valorization Process
Authors: Kang Yubin , Swain Basudev , Im Byoungyong , Yoon Jin-Ho , Park Kwang Hoon , Lee Chan Gi , Kim Dae Guen
Published: November 2019

In the current investigation, we synthesize zeolite using two different waste streams, such as aluminum dross and waste glass powder, for its potential application in indium and tin recovery from the leach liquor of waste liquid crystal display (LCD) glass. The aluminum dross (Al resource) and waste glass powder (Si resource) were used as raw materials for the synthesis of zeolite. Zeolite was synthesized using different weight ratios of Al dross and waste LCD glass by hydrothermal synthesis route using NaOH. The weight ratio variations of Al dross and waste LCD glass in this study are 0.3:1, 0.5:1, 1:1, 2:1, 3:1, and 4:1 using 2 M NaOH hydroxide by the hydrothermal technique. The synthesized zeolite was analyzed by X-ray diffraction spectroscopy (XRD), scanning electron microscopy (SEM), and Brunauer–Emmett–Teller (BET) followed by the possible application for recovery/extraction of indium and tin from leach liquor of waste LCD glasses. The indium extraction of average 93.66%, and tin extraction of 93.10% could be achieved from mixed solution indium and tin chloride. The significant achievement of the current investigation is that it can address two environment problems simultaneously, i.e., waste LCD glass and Al dross, and can be used for value recovery from waste LCD, LCD etching waste like secondary resources.
DNA Methylation Profile of β-1,3-Glucanase and Chitinase Genes in Flax Shows Specificity Towards Fusarium Oxysporum Strains Differing in Pathogenicity
Authors: Wojtasik Wioleta , Boba Aleksandra , Preisner Marta , Kostyn Kamil , Szopa Jan , Kulma Anna
Published: November 2019

Most losses in flax (Linum usitatissimum L.) crops are caused by fungal infections. The new epigenetic approach to improve plant resistance requires broadening the knowledge about the influence of pathogenic and non-pathogenic Fusarium oxysporum strains on changes in the profile of DNA methylation. Two contrasting effects on the levels of methylation in flax have been detected for both types of Fusarium strain infection: Genome-wide hypermethylation and hypomethylation of resistance-related genes (β-1,3-glucanase and chitinase). Despite the differences in methylation profile, the expression of these genes increased. Plants pretreated with the non-pathogenic strain memorize the hypomethylation pattern and then react more efficiently upon pathogen infection. The peak of demethylation correlates with the alteration in gene expression induced by the non-pathogenic strain. In the case of pathogen infection, the expression peak lags behind the gene demethylation. Dynamic changes in tetramer methylation induced by both pathogenic and non-pathogenic Fusarium strains are dependent on the ratio between the level of methyltransferase and demethylase gene expression. Infection with both Fusarium strains suppressed methyltransferase expression and increased the demethylase (demeter) transcript level. The obtained results provide important new information about changes in methylation profile and thus expression regulation of pathogenesis-related genes in the flax plant response to stressors.
Performance of an Immunochromatographic Test (ICT) in Comparison to Some Commonly Used Serological Tests for the Diagnosis of Brucellosis in Dromedary Camels (Camelus dromedarius)
Authors: Serhan Wissam S. , Khan Rashid A. , Gasim Esmat F. , Alketbi Mariam S. , De Massis Fabrizio , Calistri Paolo , Giovannini Armando , Al Hosani Mohamed A. , Al Jaberi Saleha A. , Al Mansoori Asma M. , Al Ketbi Asma S. , Khalafalla Abdelmalik I. , Almuhairi Salama S.
Published: November 2019

Serological tests may represent an essential tool for the diagnosis of camel brucellosis; however, concerns arise in the scientific community regarding the direct transposition from cattle and small ruminants without adequate validation. The present study was made to compare four serological tests for the diagnosis of brucellosis in dromedary camels (Camelus dromedarius). In terms of sensitivity, our results show that the Immunochromatographic Test (ICT) shows the higher value of sensitivity, 98.67% (95% Confidence Level (C.L): 94.36–99.99%), followed by the Fluorescence Polarization Assay (FPA) with 95.05% (95% C.L: 88.23–99.51%), then the Competitive Enzyme-Linked Immunosorbent Assay (c-ELISA) with 94.94% (95% C.L: 88.25–99.45%) and, finally, the Rose Bengal Test (RBT) with 68.95% (95% C.L: 56.55–80.69%), which is the only test showing a significantly lower sensitivity compared to the others. On the other hand, our study revealed no significant difference in terms of specificity between all the tests under study, with a range from 99.06% (95% C.L: 98.34–99.64%) for the ICT to 99.92% (95% C.L: 99.64–100%) for the RBT. The ICT was found to be comparable in terms of sensitivity and specificity with the most commonly used tests for camel brucellosis. The results of the present study are of paramount importance for designing surveillance and control measures for brucellosis in camel populations.
Ancient Melt Depletion and Metasomatic History of the Subduction Zone Mantle: Osmium Isotope Evidence of Peridotites from the Yap Trench, Western Pacific
Authors: Chen Ling , Tang Limei , Li Xiaohu , Zhang Jie , Wang Wei , Li Zhenggang , Wang Hao , Wu Xichang , Chu Fengyou
Published: November 2019

Highly depleted peridotites from the Yap Trench in the western Pacific Ocean have been studied for Re-Os elements and Re-Os isotopes. These peridotites have a low Re-Os content and variable 187Os/188Os ratios (0.12043–0.14867). The highest 187Os/188Os ratio is far higher than that of the primitive upper mantle and the lowest 187Os/188Os ratio is comparable to the most unradiogenic 187Os/188Os ratio (0.11933) discovered in subduction zone peridotites. The suprachondritic 187Os/188Os ratios of the Yap Trench peridotites results from modification of the mantle wedge by slab-derived fluid and melt. This is consistent with the observation that high 187Os/188Os ratios generally occur in oceanic peridotites with low Os content (<2 ppb) since Os may be reduced during late processes such as fluid alteration and melt refertilization. The sub-chondritic 187Os/188Os ratios of the Yap Trench peridotites correspond to a Re depletion age of 0.24–1.16 billion years, which means that these peridotites represent old mantle residue of ancient melting events. This ancient melting, combined with probable back-arc melting and forearc melting during subduction initiation, indicates that the Yap Trench mantle has a complex evolutionary history. The amount of old mantle residue in the oceanic asthenosphere was underestimated because the 187Os/188Os ratio in mantle peridotites is elevated during late processes. Therefore, old depleted mantle fragments may contribute substantially to the chemical heterogeneity of the oceanic mantle.
CNL–Clitocybe nebularis Lectin—The Fungal GalNAcβ1-4GlcNAc-Binding Lectin
Authors: Sabotič Jerica , Kos Janko
Published: November 2019

Clitocybe nebularis lectin (CNL) is present in fruiting bodies of clouded agaric along with several similar isolectins that are all small and stable proteins. It is a beta-trefoil type lectin forming homodimers that are essential for its functionality. It binds specifically N,N′-diacetyllactosediamine (GalNAcβ1-4GlcNAc, LacDiNac) and human blood group A determinant-containing glycan epitopes. Its most probable function is to defend fruiting bodies against predators and parasites. In addition, an endogenous regulatory function is possible for CNL, as indicated by its interaction with fungal protease inhibitors sharing the beta-trefoil fold. CNL is toxic to insects, nematodes and amoebae, as well as to leukemic T-cell lines. Bivalent carbohydrate binding is essential for the toxicity of CNL, against both invertebrates and cancer-derived cell lines. In addition, CNL exhibits potent immunostimulation of human dendritic cells, resulting in a strong T helper cell type 1 response. Based on its unique characteristics, CNL is a promising candidate for applications in human and veterinary medicine as well as in agriculture, for plant protection.
Efficient Removal of Copper Ion from Wastewater Using a Stable Chitosan Gel Material
Authors: Yang Zujin , Chai Yuxin , Zeng Lihua , Gao Zitao , Zhang Jianyong , Ji Hongbing
Published: November 2019

Gel adsorption is an efficient method for the removal of metal ion. In the present study, a functional chitosan gel material (FCG) was synthesized successfully, and its structure was detected by different physicochemical techniques. The as-prepared FCG was stable in acid and alkaline media. The as-prepared material showed excellent adsorption properties for the capture of Cu2+ ion from aqueous solution. The maximum adsorption capacity for the FCG was 76.4 mg/g for Cu2+ ion (293 K). The kinetic adsorption data fits the Langmuir isotherm, and experimental isotherm data follows the pseudo-second-order kinetic model well, suggesting that it is a monolayer and the rate-limiting step is the physical adsorption. The separation factor (RL) for Langmuir and the 1/n value for Freundlich isotherm show that the Cu2+ ion is favorably adsorbed by FCG. The negative values of enthalpy (ΔH°) and Gibbs free energy (ΔG°) indicate that the adsorption process are exothermic and spontaneous in nature. Fourier transform infrared (FTIR) spectroscopy and x-ray photoelectron spectroscopy (XPS) analysis of FCG before and after adsorption further reveal that the mechanism of Cu2+ ion adsorption. Further desorption and reuse experiments show that FCG still retains 96% of the original adsorption following the fifth adsorption–desorption cycle. All these results indicate that FCG is a promising recyclable adsorbent for the removal of Cu2+ ion from aqueous solution.
Screening Effective Antifungal Substances from the Bark and Leaves of Zanthoxylum avicennae by the Bioactivity-Guided Isolation Method
Authors: Xiong Yongtong , Huang Guan , Yao Zongli , Zhao China , Zhu Xiang , Wu Qinglai , Zhou Xudong , Li Junkai
Published: November 2019

To find good antifungal substances by the bioactivity-guided isolation method, we tracked down the effective antifungal substances in the bark and leaves of Zanthoxylum avicennae, and isolated three antifungal compounds 1, 2, and 3. The structures were identified as xanthyletin, luvangetin, and avicennin by 1H-NMR, 13C-NMR, and HRMS spectra. Particularly, compound 2 had several isomers, and the 1H-NMR spectra of 2 in different solvents showed a significant difference. To determine the stereo structure of 2, a single crystal was prepared and identified by X-ray diffraction as Luvangetin. Moreover, the difference of 1H-NMR data of 2 between in solvent dimethyl sulfoxide-d6 (DMSO-d6) and deuterated chloroform (CDCl3), and other reported isomers were discussed for the first time. The bioassay results indicated that the three compounds 1, 2, and 3 displayed low to high antifungal activities against tested phytopathogenic fungi. In particular, all compounds 1, 2, and 3 showed excellent antifungal activities against Pyricularia oryzae and Z. avicennae, with the values of half maximal effective concentration (EC50) ranging from 31 to 61 mg/L, and compound 3 was also identified as a more potent inhibitor against Fusaium graminearum (EC50 = 43.26 ± 1.76 mg/L) compared with fungicide PCA (phenazine-1-carboxylic acid) (EC50 = 52.34 ± 1.53 mg/L). The results revealed that compounds 1, 2, and 3 were the main antifungal substances of Z. avicennae, and can be used as lead compounds of a fungicide, which has good development value and prospect.
Comprehensive Analysis of the Copper Exchange Implemented in Ammonia and Protonated Forms of Mordenite Using Microwave and Conventional Methods
Authors: Shelyapina Marina G. , Krylova Ekaterina A. , Zhukov Yurii M. , Zvereva Irina A. , Rodriguez-Iznaga Inocente , Petranovskii Vitalii , Fuentes-Moyado Sergio
Published: November 2019

This article presents the results of a comprehensive study of copper-exchanged mordenite samples prepared from its ammonia and protonated forms (Si/Al = 10) using two different ion exchange methods: conventional and microwave (MW)-assisted. The protonated H-MOR-10 sample was obtained by calcination of commercial NH4-MOR-10; in this case, a slight degradation of the mordenite framework was observed, but the resulting defects were partially restored after the first ion-exchange procedure of protons for copper ions. The level of copper exchange in the studied materials was found to be limited to 70%. Regardless of the exchange procedure, the replacement of ammonium or proton ions with copper led to a linear increase in the a/b ratio of cell parameters in accordance with an increase in the level of copper exchange, which means that all Cu2+ cations are ion-exchangeable and enter the main mordenite channel. Thermal analysis indicated a correlation between the replacement of various ammonium and hydroxyl groups by copper ions during the exchange treatment and their dehydroxylation energy during thermal decomposition. As a conclusion: MW-assisted treatment proved itself as an efficacious method for the synthesis of copper-exchanged mordenites, which not only significantly reduces preparation time but leads to a systematically higher copper exchange level.
Contribution of Berry Polyphenols to the Human Metabolome
Authors: Chandra Preeti , Rathore Atul S. , Kay Kristine L. , Everhart Jessica L. , Curtis Peter , Burton-Freeman Britt , Cassidy Aedin , Kay Colin D.
Published: November 2019

Diets rich in berries provide health benefits, however, the contribution of berry phytochemicals to the human metabolome is largely unknown. The present study aimed to establish the impact of berry phytochemicals on the human metabolome. A “systematic review strategy” was utilized to characterize the phytochemical composition of the berries most commonly consumed in the USA; (poly)phenols, primarily anthocyanins, comprised the majority of reported plant secondary metabolites. A reference standard library and tandem mass spectrometry (MS/MS) quantitative metabolomics methodology were developed and applied to serum/plasma samples from a blueberry and a strawberry intervention, revealing a diversity of benzoic, cinnamic, phenylacetic, 3-(phenyl)propanoic and hippuric acids, and benzyldehydes. 3-Phenylpropanoic, 2-hydroxybenzoic, and hippuric acid were highly abundant (mean > 1 µM). Few metabolites at concentrations above 100 nM changed significantly in either intervention. Significant intervention effects (p < 0.05) were observed for plasma/serum 2-hydroxybenzoic acid and hippuric acid in the blueberry intervention, and for 3-methoxyphenylacetic acid and 4-hydroxyphenylacetic acid in the strawberry intervention. However, significant within-group effects for change from baseline were prevalent, suggesting that high inter-individual variability precluded significant treatment effects. Berry consumption in general appears to cause a fluctuation in the pools of small molecule metabolites already present at baseline, rather than the appearance of unique berry-derived metabolites, which likely reflects the ubiquitous nature of (poly)phenols in the background diet.
Composition of Sugars in Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.)
Authors: Vilkickyte Gabriele , Raudonis Raimondas , Motiekaityte Vida , Vainoriene Rimanta , Burdulis Deividas , Viskelis Jonas , Raudone Lina
Published: November 2019

Products of lingonberries are widely used in the human diet; they are also promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries. It is important to examine the sugar profile of these berries, due to potential deleterious health effects resulting from high sugar consumption. The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC–ELSD method of analysis. Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved. The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries. Cultivar ‘Erntekrone’ and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars. The changes in the sugar levels during the growing season were apparent in lingonberries and the highest amounts accumulated at the end of the vegetation. According to our findings, lingonberries seem to be an appropriate source of dietary sugars.
Synthesis of Aryl Propionamide Scaffold Containing a Pentafluorosulfanyl Moiety as SARMs
Authors: Shao Pingxuan , Zhou Yan , Yang Dehua , Wang Ming-Wei , Lu Wei , Jin Jiyu
Published: November 2019

The pentafluorosulfane (SF5) group, as a more electronegative bioisostere than the trifluoromethyl (CF3) group, has been gaining greater attention and increasingly reported usage in medicinal chemistry. Ostarine is the selective androgen receptor modulators (SARMs) containing a CF3 group in clinical trial III. In this study, 21 ostarine derivatives for replacing the CF3 group with SF5 substituents were synthesized. Some SF5-derivatives showed androgen receptor (AR) agonistic activities in vitro. The results pointed to the potential of using this scaffold to develop new AR agonists.
Facile Synthesis of Water-Soluble Fullerene (C60) Nanoparticles via Mussel-Inspired Chemistry as Efficient Antioxidants
Authors: Zhang Xiaoyan , Ma Yihan , Fu Sheng , Zhang Aiqing
Published: November 2019

Rational design and modification of the all-carbon fullerene cages to meliorate their nature of hydrophobicity is critical for biomedical applications. The outstanding electron affinity of fullerenes enables them to effectively eliminate reactive oxygen species (ROS), the excess of which may lead to health hazards or biological dysfunction. Herein reported is a facile, mild, and green approach to synthesizing the favorable water-soluble C60 nanoparticles capable of ROS-scavenging by combining the mussel-inspired chemistry with the Michael addition reaction. Various characterization techniques, including Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectra (XPS), thermogravimetric analysis (TGA), transmission electron cryomicroscopy (Cryo-TEM), and dynamic laser scattering (DLS) were carried out to confirm the satisfactory preparation of the hybrid C60-PDA-GSH nanoparticles, which exhibited apparent scavenging capacity of DPPH and hydroxyl radicals in vitro. Additionally, the biocompatible C60-PDA-GSH nanoparticles entered into cells and displayed a universal cytoprotective effect against oxidative press induced by H2O2 in four kinds of human cells at a low concentration of 2 μg/mL. The ease and versatility of the strategy present in this work will not only trigger more fullerene-based materials by the immobilization of diverse functional molecules, but will also extend their possible applications.
Vortex Beam Encoded All-Optical Logic Gates Based on Nano-Ring Plasmonic Antennas
Authors: Liu Houquan , Deng Hongchang , Deng Shijie , Teng Chuanxin , Chen Ming , Yuan Libo
Published: November 2019

Vortex beam encoded all-optical logic gates are suggested to be very important in future information processing. However, within current logic devices, only a few are encoded by using vortex beams and, in these devices, some space optical elements with big footprints (mirror, dove prism and pentaprism) are indispensable components, which is not conducive to device integration. In this paper, an integrated vortex beam encoded all-optical logic gate based on a nano-ring plasmonic antenna is proposed. In our scheme, by defining the two circular polarization states of the input vortex beams as the input logic states and the normalized intensity of the plasmonic field at the center of the nano-ring as the output logic states, OR and AND (NOR and NAND) logic gates are realized when two 1st (1st) order vortex beams are chosen as the two input signals; and a NOT logic gate is obtained when one 1st order vortex beam is chosen as the input signal. In addition, by defining the two linear polarization states (x and y polarization) of the input vortex beams as the two input logic states, an XNOR logic gate is realized when two 1st order vortex beams are chosen as the two input signals.
Serological and Molecular Identification of Brucella spp. in Pigs from Cairo and Giza Governorates, Egypt
Authors: Khan Aman Ullah , Melzer Falk , El-Soally Sherif Abdel Ghafar Elsayed , Elschner Mandy C. , Mohamed Shereen Aziz , Sayed Ahmed Mohamed Abdelmonem , Roesler Uwe , Neubauer Heinrich , El-Adawy Hosny
Published: November 2019

Brucellosis is considered as endemic disease of animals and humans since thousands of years in Egypt. However, brucellosis in pigs has never been reported in Egypt. Thus, serological and molecular assays were applied to detect anti-Brucella antibodies and DNA in serum samples collected from pigs. In total 331 blood samples collected from male and female pigs at slaughterhouses of Cairo and Giza governorates were investigated using Brucella c- and i-ELISA and Brucella real-time PCR. Anti-Brucella antibodies were detected in 16 (4.83%) and 36 (10.8%) sera by i-ELISA and c-ELISA, respectively. Brucella DNA was detected in 10 (3.02%) seropositive samples and identified as Brucella melitensis (7/10) and Brucella suis (3/10). A higher prevelance was found in boars. This is the first study investigating pig brucellosis in Egypt. The results of this study will raise awareness for brucellosis in these farm animals and will help to develop effective control strategies.
From Stable to Lab—Investigating Key Factors for Sudden Deaths Caused by Streptococcus suis
Authors: Hennig-Pauka Isabel , Imker Rabea , Mayer Leonie , Brügmann Michael , Werckenthin Christiane , Weber Heike , Menrath Andrea , de Buhr Nicole
Published: November 2019

Swine stocks are endemically infected with the major porcine pathogen Streptococcus (S.) suis. The factors governing the transition from colonizing S. suis residing in the tonsils and the exacerbation of disease have not yet been elucidated. We analyzed the sudden death of fattening pigs kept under extensive husbandry conditions in a zoo. The animals died suddenly of septic shock and showed disseminated intravascular coagulopathy. Genotypic and phenotypic characterizations of the isolated S. suis strains, a tonsillar isolate and an invasive cps type 2 strain, were conducted. Isolated S. suis from dead pigs belonged to cps type 2 strain ST28, whereas one tonsillar S. suis isolate harvested from a healthy animal belonged to ST1173. Neither S. suis growth, induction of neutrophil extracellular traps, nor survival in blood could explain the sudden deaths. Reconstituted blood assays with serum samples from pigs of different age groups from the zoo stock suggested varying protection of individuals against pathogenic cps type 2 strains especially in younger pigs. These findings highlight the benefit of further characterization of the causative strains in each case by sequence typing before autologous vaccine candidate selection.
Persistent Listeria monocytogenes Isolates from a Poultry-Processing Facility Form More Biofilm but Do Not Have a Greater Resistance to Disinfectants than Sporadic Strains
Authors: Rodríguez-Campos Daniel , Rodríguez-Melcón Cristina , Alonso-Calleja Carlos , Capita Rosa
Published: November 2019

Some strains of Listeria monocytogenes can persist in food-processing environments, increasing the likelihood of the contamination of foodstuffs. To identify traits that contribute to bacterial persistence, a selection of persistent and sporadic L. monocytogenes isolates from a poultry-processing facility was investigated for biofilm-forming ability (crystal violet assay). The susceptibility of sessile cells to treatments (five minutes) with sodium hypochlorite having 10% active chlorine (SHY: 10,000 ppm, 25,000 ppm, and 50,000 ppm) and benzalkonium chloride (BZK: 2500 ppm, 10,000 ppm, and 25,000 ppm) was also studied. All isolates exhibited biofilm formation on polystyrene. Persistent strains showed larger (p < 0.001) biofilm formation (OD580 = 0.301 ± 0.097) than sporadic strains (OD580 = 0.188 ± 0.082). A greater susceptibility to disinfectants was observed for biofilms of persistent strains than for those of sporadic strains. The application of SHY reduced biofilms only for persistent strains. BZK increased OD580 in persistent strains (2500 ppm) and in sporadic strains (all concentrations). These results indicate that the use of BZK at the concentrations tested could represent a public health risk. Findings in this work suggest a link between persistence and biofilm formation, but do not support a relationship between persistence and the resistance of sessile cells to disinfectants.
Impact of a Live Attenuated Classical Swine Fever Virus Introduced to Jeju Island, a CSF-Free Area
Authors: Choe SeEun , Kim Jae-Hoon , Kim Ki-Sun , Song Sok , Kang Wan-Choul , Kim Hyeon-Ju , Park Gyu-Nam , Cha Ra Mi , Cho In-Soo , Hyun Bang-Hun , Park Bong-Kyun , An Dong-Jun
Published: November 2019

Here, we examine the effects of LOM(Low virulence of Miyagi) strains isolated from pigs (Jeju LOM strains) of Jeju Island, where vaccination with a live attenuated classical swine fever (CSF) LOM vaccine strain was stopped. The circulation of the Jeju LOM strains was mainly caused by a commercial swine erysipelas (Erysipelothrix rhusiopathiae) vaccine mixed with a LOM vaccine strain, which was inoculated into pregnant sows of 20 pig farms in 2014. The Jeju LOM strain was transmitted to 91 pig farms from 2015 to 2018. A histopathogenic investigation was performed for 25 farms among 111 farms affected by the Jeju LOM strain and revealed pigs infected with the Jeju LOM strain in combination with other pathogens, which resulted in the abortion of fetuses and mortality in suckling piglets. Histopathologic examination and immunohistochemical staining identified CSF-like lesions. Our results also confirm that the main transmission factor for the Jeju LOM strain circulation is the vehicles entering/exiting farms and slaughterhouses. Probability estimates of transmission between cohabiting pigs and pigs harboring the Jeju LOM strain JJ16LOM-YJK08 revealed that immunocompromised pigs showed horizontal transmission (r = 1.22). In a full genome analysis, we did not find genetic mutation on the site that is known to relate to pathogenicity between Jeju LOM strains (2014–2018) and the commercial LOM vaccine strain. However, we were not able to determine whether the Jeju LOM strain (2014–2018) is genetically the same virus as those of the commercial LOM vaccine due to several genetic variations in structure and non-structure proteins. Therefore, further studies are needed to evaluate the pathogenicity of the Jeju LOM strain in pregnant sow and SPF pigs and to clarify the characteristics of Jeju LOM and commercial LOM vaccine strains.
Host Range of the Mikrocytid Parasite Paramikrocytos canceri in Decapod Crustaceans
Authors: Edwards Morgan , Coates Christopher , Rowley Andrew
Published: November 2019

Mikrocytids are a widespread but rather neglected group of parasites of aquatic invertebrates. One such parasite is Paramikrocytos canceri—discovered to infect the antennal gland of the juvenile edible crab, Cancer pagurus, taken from several intertidal sites across the United Kingdom. To determine if this parasite is also present in other species of decapod crustaceans, we surveyed crabs (n = 330) across two contrasting sites in Pembrokeshire (UK). Using a histopathological approach, P. canceri infection was confirmed in variable numbers of edible crabs from both survey sites, 7–44%. No measurable signs of infection were encountered in four other co-located species, including European shore crabs (Carcinus maenas), Montagu’s crabs (Xantho hydrophilus), velvet swimming crabs (Necora puber) and broad-clawed porcelain crabs (Porcellana platycheles). These data imply that P. canceri has a more limited host range than suggested by molecular diagnosis alone.
Development of Functionalized Carbon Nano-Onions Reinforced Zein Protein Hydrogel Interfaces for Controlled Drug Release
Authors: Mamidi Narsimha , González-Ortiz Aldo , Lopez Romo Irasema , V. Barrera Enrique
Published: November 2019

In the current study, poly 4-mercaptophenyl methacrylate-carbon nano-onions (PMPMA-CNOs = f-CNOs) reinforced natural protein (zein) composites (zein/f-CNOs) are fabricated using the acoustic cavitation technique. The influence of f-CNOs inclusion on the microstructural properties, morphology, mechanical, cytocompatibility, in-vitro degradation, and swelling behavior of the hydrogels are studied. The tensile results showed that zein/f-CNOs hydrogels fabricated by the acoustic cavitation system exhibited good tensile strength (90.18 MPa), compared with the hydrogels fabricated by the traditional method and only microwave radiation method. It reveals the magnitude of physisorption and degree of colloidal stability of f-CNOs within the zein matrix under acoustic cavitation conditions. The swelling behaviors of hydrogels were also tested and improved results were noticed. The cytotoxicity of hydrogels was tested with osteoblast cells. The results showed good cell viability and cell growth. To explore the efficacy of hydrogels as drug transporters, 5-fluorouracil (5-FU) release was measured under gastric and intestinal pH environment. The results showed pH-responsive sustained drug release over 15 days of study, and pH 7.4 showed a more rapid drug release than pH 2.0 and 4.5. Nonetheless, all the results suggest that zein/f-CNOs hydrogel could be a potential pH-responsive drug transporter for a colon-selective delivery system.
Rituals and Machines: A Confucian Response to Technology-Driven Moral Deskilling
Authors: Wong Pak-Hang
Published: November 2019

Robots and other smart machines are increasingly interwoven into the social fabric of our society, with the area and scope of their application continuing to expand. As we become accustomed to interacting through and with robots, we also begin to supplement or replace existing human–human interactions with human–machine interactions. This article aims to discuss the impacts of the shift from human–human interactions to human–machine interactions in one facet of our self-constitution, i.e., morality. More specifically, it sets out to explore whether and how the shift to human–machine interactions can affect our moral cultivation. I shall structure the article around what Shannon Vallor calls technology-driven moral deskilling, i.e., the phenomenon of technology negatively affecting individual moral cultivation, and shall also attempt to offer a Confucian response to the problem. I first elaborate in detail Vallor’s idea of technology-driven moral deskilling. Next, I discuss three paradigms of virtue acquisition identified by Nancy E. Snow, i.e., the “folk” paradigm, the skill-and-expertise paradigm, and the Confucian paradigm, and show how the Confucian paradigm can help us to respond to technology-driven moral deskilling. Finally, I introduce the idea of Confucian rituals (li) and argue for the ritualizing of machines as an answer to technology-driven moral deskilling.
Identification, Evolution, and Expression Profiling of Histone Lysine Methylation Moderators in Brassica rapa
Authors: Liu Gaofeng , Khan Nadeem , Ma Xiaoqing , Hou Xilin
Published: November 2019

Histone modifications, such as methylation and demethylation, are vital for regulating chromatin structure, thus affecting its expression patterns. The objective of this study is to understand the phylogenetic relationships, genomic organization, diversification of motif modules, gene duplications, co-regulatory network analysis, and expression dynamics of histone lysine methyltransferases and histone demethylase in Brassica rapa. We identified 60 SET (HKMTases), 53 JmjC, and 4 LSD (HDMases) genes in B. rapa. The domain composition analysis subcategorized them into seven and nine subgroups, respectively. Duplication analysis for paralogous pairs of SET and JmjC (eight and nine pairs, respectively) exhibited variation. Interestingly, three pairs of SET exhibited Ka/Ks > 1.00 values, signifying positive selection, whereas the remaining underwent purifying selection with values less than 1.00. Furthermore, RT-PCR validation analysis and RNA-sequence data acquired on six different tissues (i.e., leaf, stem, callus, silique, flower, and root) revealed dynamic expression patterns. This comprehensive study on the abundance, classification, co-regulatory network analysis, gene duplication, and responses to heat and cold stress of SET and JmjC provides insights into the structure and diversification of these family members in B. rapa. This study will be helpful to reveal functions of these putative SET and JmjC genes in B. rapa.
Study on Sea Clutter Suppression Methods Based on a Realistic Radar Dataset
Authors: Lv Mingjie , Zhou Chen
Published: November 2019

To improve the ability of radar to detect targets such as ships with a background of strong sea clutter, different sea-clutter suppression algorithms are developed based on the realistic Intelligent PIxel processing X-band (IPIX) radar datasets, and quantitative research is carried out. Four algorithms, namely root cycle cancellation, singular-value decomposition (SVD) suppression, wavelet weighted reconstruction, and empirical mode decomposition (EMD) weighted reconstruction and their corresponding suppression methods are introduced. Then, the differences between the four algorithms before and after sea-clutter suppression are compared and analyzed. The average clutter-suppression and target-suppression amplitudes are selected as measures to verify the suppression effect. Sea-clutter data collected in the high-sea state, low-sea state, near-sea area, and far-sea area are selected for statistical analysis after suppression. All four methods have certain suppression effects, among which EMD reconstruction is best, reaching an average clutter-suppression range of 15.507 dB and a signal-suppression range of about 1 dB, which can improve the ability of radar to detect targets such as ships with a background of strong sea clutter.
UBathy: A New Approach for Bathymetric Inversion from Video Imagery
Authors: Simarro Gonzalo , Calvete Daniel , Luque Pau , Orfila Alejandro , Ribas Francesca
Published: November 2019

A new approach to infer the bathymetry from coastal video monitoring systems is presented. The methodology uses principal component analysis of the Hilbert transform of video images to obtain the components of the wave propagation field and their corresponding frequency and wavenumber. Incident and reflected constituents and subharmonics components are also found. Local water depth is then successfully estimated through wave dispersion relationship. The method is first applied to monochromatic and polychromatic synthetic wave trains propagated using linear wave theory over an alongshore uniform bathymetry in order to analyze the influence of different parameters on the results. To assess the ability of the approach to infer the bathymetry under more realistic conditions and to explore the influence of other parameters, nonlinear wave propagation is also performed using a fully nonlinear Boussinesq-type model over a complex bathymetry. In the synthetic cases, the relative root mean square error obtained in bathymetry recovery (for water depths 0.75 m ⩽ h ⩽ 8.0 m ) ranges from ∼1% to ∼3% for infinitesimal-amplitude wave cases (monochromatic or polychromatic) to ∼15% in the most complex case (nonlinear polychromatic waves). Finally, the new methodology is satisfactorily validated through a real field site video.
Characterization of the Kinematics of Three Bears Landslide in Northern California Using L-band InSAR Observations
Authors: Liu Yuanyuan , Lu Zhong , Zhao Chaoying , Kim Jinwoo , Zhang Qin , de la Fuente Juan
Published: November 2019

High-precision monitoring of landslides is essential for understanding their kinematics and reducing landslide induced damage. The spatiotemporal deformation variations of the Three Bears landslide in northern California have not been systematically monitored and interpreted. In this study, we applied advanced time-series InSAR analysis methods to characterize the kinematics of the landslide covering two periods (2007–2011 and 2015–2017) with multi-track synthetic aperture radar (SAR) images acquired from L-band ALOS PALSAR-1/2 satellites. Our results show that the Three Bears landslide has been moving consistently, with the Line of Sight (LOS) deformation rate exceeding 300 mm/yr from 2007 to 2011 and around 250 mm/yr from 2015 to 2017. The east–west and vertical deformation components were inverted by integrating ascending and descending ALOS PALSAR-2 interferograms during the 2015–2017 period, indicating that the landslide was dominated by eastward movement and in a continuous deformation stage. Down-slope landslide motions observed from adjacent satellite tracks with slightly different radar look vectors were used to verify the accuracy of InSAR-derived results. Comparison between linearly detrended InSAR displacements and precipitation records indicate that the landslide tends to accelerate during the wet seasons. The results could allow us to better understand the kinematics of the landslide and provide significant evidence for evaluating the potential for catastrophic failure and the threat posed by such failure to human life and property. Combined with a proper geotechnical/geomechanical model, the results would also facilitate the design and implementation of mitigation measures.
A New Algorithm for the Retrieval of Atmospheric Profiles from GNSS Radio Occultation Data in Moist Air and Comparison to 1DVar Retrievals
Authors: Li Ying , Kirchengast Gottfried , Scherllin-Pirscher Barbara , Schwaerz Marc , Nielsen Johannes K. , Ho Shu-peng , Yuan Yun-bin
Published: November 2019

The Global Navigation Satellite System (GNSS) Radio Occultation (RO) is a key technique for obtaining thermodynamic profiles of temperature, humidity, pressure, and density in the Earth’s troposphere. However, due to refraction effects of both the dry air and water vapor at low altitudes, retrieval of accurate profiles is challenging. Here we introduce a new moist air retrieval algorithm aiming to improve the quality of RO-retrieved profiles in moist air and including uncertainty estimation in a clear sequence of steps. The algorithm first uses RO dry temperature and pressure and background temperature/humidity and their uncertainties to retrieve humidity/temperature and their uncertainties. These temperature and humidity profiles are then combined with their corresponding background profiles by optimal estimation employing inverse-variance weighting. Finally, based on the optimally estimated temperature and humidity profiles, pressure and density profiles are computed using hydrostatic and equation-of-state formulas. The input observation and background uncertainties are dynamically estimated, accounting for spatial and temporal variations. We show results from applying the algorithm on test datasets, deriving insights from both individual profiles and statistical ensembles, and from comparison to independent 1D-Variational (1DVar) algorithm-derived moist air retrieval results from Radio Occultation Meteorology Satellite Application Facility Copenhagen (ROM-SAF) and University Corporation for Atmospheric Research (UCAR) Boulder RO processing centers. We find that the new scheme is comparable in its retrieval performance and features advantages in the integrated uncertainty estimation that includes both estimated random and systematic uncertainties and background bias correction. The new algorithm can therefore be used to obtain high-quality tropospheric climate data records including uncertainty estimation.
A Laser-Based On-Machine Measuring System for Profile Accuracy of Double-Headed Screw Rotor
Authors: Dong Zhixu , Xu Fangsu , Sun Xingwei , Liu Weijun
Published: November 2019

Great length, large weight and other factors may cause difficulty in measuring the profile accuracy of the double-headed screw rotor. To solve this problem, an on-machine measuring system based on a laser-displacement sensor (LDS) was designed and implemented in this paper by taking an LXK100 four-axis whirlwind milling machine as the carrier. To improve the measurement accuracy of the system, the generalized variable-structural-element morphological method, polynomial interpolation algorithm and ellipse fitting method were first combined to realize the rapid subpixel centroid extraction from a noise-containing spot image, thus improving the data acquisition accuracy of the LDS, and then the hybrid method was experimentally verified. Next, a wavelet threshold function with high-order differentiability and adaptive wavelet coefficient contractility was constructed based on the hyperbolic tangent function, so as to inhibit the disturbance from random errors and preserve real profile information, and this method was simulated and verified. Subsequently, a smoothing algorithm for point cloud data was proposed based on the Lagrange multiplier method to avoid the defect of the piecewise curve-fitting method, that is, function continuity and differentiability could not be satisfied at piecewise points. Finally, the profile accuracy was calculated in real time according to the data reconstruction result and the machining quality was judged. The measurement experiment of the double-headed screw rotor indicates that the proposed on-machine measuring system can complete the profile accuracy measurement for a screw pitch within 39.7 s with measurement accuracy reaching ±8 μm, and the measurement uncertainties of the major axis, minor axis and screw pitch are 0.72 μm, 0.69 μm and 1.24 μm, respectively. Therefore, the measurement accuracy and efficiency are both remarkably improved.