bluuebunny/crossref_metadata_2025 huggingface dataset:
Interleukin-1 receptor accessory protein blockade limits the development of atherosclerosis and reduces plaque inflammation
Authors: Mulholland Megan , Depuydt Marie A C , Jakobsson Gabriel , Ljungcrantz Irena , Grentzmann Andrietta , To Fong , Bengtsson Eva , Jaensson Gyllenbäck Elin , Grönberg Caitríona , Rattik Sara , Liberg David , Schiopu Alexandru , Björkbacka Harry , Kuiper Johan , Bot Ilze , Slütter Bram , Engelbertsen Daniel
Published: April 2024

Aims

The interleukin-1 receptor accessory protein (IL1RAP) is a co-receptor required for signalling through the IL-1, IL-33, and IL-36 receptors. Using a novel anti-IL1RAP-blocking antibody, we investigated the role of IL1RAP in atherosclerosis.

Methods and results

Single-cell RNA sequencing data from human atherosclerotic plaques revealed the expression of IL1RAP and several IL1RAP-related cytokines and receptors, including IL1B and IL33. Histological analysis showed the presence of IL1RAP in both the plaque and adventitia, and flow cytometry of murine atherosclerotic aortas revealed IL1RAP expression on plaque leucocytes, including neutrophils and macrophages. High-cholesterol diet fed apolipoprotein E–deficient (Apoe−/−) mice were treated with a novel non-depleting IL1RAP-blocking antibody or isotype control for the last 6 weeks of diet. IL1RAP blockade in mice resulted in a 20% reduction in subvalvular plaque size and limited the accumulation of neutrophils and monocytes/macrophages in plaques and of T cells in adventitia, compared with control mice. Indicative of reduced plaque inflammation, the expression of several genes related to leucocyte recruitment, including Cxcl1 and Cxcl2, was reduced in brachiocephalic arteries of anti-IL1RAP-treated mice, and the expression of these chemokines in human plaques was mainly restricted to CD68+ myeloid cells. Furthermore, in vitro studies demonstrated that IL-1, IL-33, and IL-36 induced CXCL1 release from both macrophages and fibroblasts, which could be mitigated by IL1RAP blockade.

Conclusion

Limiting IL1RAP-dependent cytokine signalling pathways in atherosclerotic mice reduces plaque burden and plaque inflammation, potentially by limiting plaque chemokine production.
CoMentG: comprehensive retrieval of generic relationships between biomedical concepts from the scientific literature
Authors: Novoa Jorge , López-Ibáñez Javier , Chagoyen Mónica , Ranea Juan A G , Pazos Florencio
Published: April 2024

The CoMentG resource contains millions of relationships between terms of biomedical interest obtained from the scientific literature. At the core of the system is a methodology for detecting significant co-mentions of concepts in the entire PubMed corpus. That method was applied to nine sets of terms covering the most important classes of biomedical concepts: diseases, symptoms/clinical signs, molecular functions, biological processes, cellular compartments, anatomic parts, cell types, bacteria and chemical compounds. We obtained more than 7 million relationships between more than 74 000 terms, and many types of relationships were not available in any other resource. As the terms were obtained from widely used resources and ontologies, the relationships are given using the standard identifiers provided by them and hence can be linked to other data. A web interface allows users to browse these associations, searching for relationships for a set of terms of interests provided as input, such as between a disease and their associated symptoms, underlying molecular processes or affected tissues. The results are presented in an interactive interface where the user can explore the reported relationships in different ways and follow links to other resources.

Database URL: https://csbg.cnb.csic.es/CoMentG/
European Association of Cardiovascular Imaging survey on cardiovascular multimodality imaging in acute myocarditis
Authors: Bohbot Yohann , Pezel Théo , Demirkıran Ahmet , Androulakis Emmanuel , Houshmand Golnaz , Szabo Liliana , Manka Robert , Lembo Maria , Botezatu Simona B , Rodríguez-Palomares José F , Biering-Sørensen Tor , Podlesnikar Tomaz , Dweck Marc R
Published: April 2024

Aims

To assess the current role of cardiac imaging in the diagnosis, management, and follow-up of patients with acute myocarditis (AM) through a European Association of Cardiovascular Imaging survey.

Methods and results

A total of 412 volunteers from 74 countries responded to the survey. Most participants worked in tertiary centres (56%). All participants had access to echocardiography, while 79 and 75% had access to cardiac computed tomography angiography (CCTA) and cardiac magnetic resonance (CMR), respectively. Less than half (47%) had access to myocardial biopsy, and only 5% used this test routinely. CMR was performed within 7 days of presentation in 73% of cases. Non-ischaemic late gadolinium enhancement (LGE, 88%) and high-signal intensity in T2-weighted images (74%) were the most used diagnostic criteria for AM. CCTA was preferred to coronary angiography by 47% of participants to exclude coronary artery disease. Systematic prescription of beta-blockers and angiotensin-converting enzyme inhibitors was reported by 38 and 32% of participants. Around a quarter of participants declared considering LGE burden as a reason to treat. Most participants (90%) reported performing a follow-up echocardiogram, while 63% scheduled a follow-up CMR. The main reason for treatment discontinuation was improvement of left ventricular ejection fraction (89%), followed by LGE regression (60%). In two-thirds of participants, the decision to resume high-intensity sport was influenced by residual LGE.

Conclusion

This survey confirms the high utilization of cardiac imaging in AM but reveals major differences in how cardiac imaging is used and how the condition is managed between centres, underlining the need for recommendation statements in this topic.
Palpitations in puerperium—a self-recorded smart watch ECG gives the hint to hormone-induced ventricular arrhythmia: case report
Authors: Jankowska Paulina Anna , Georgi Christian , Bannehr Marwin , Butter Christian
Published: April 2024

Background

Gender-related aspects in cardiac arrhythmias have gained increasing attention, still the understanding of peripartum electrical disorders remains vague.

Case summary

A 28-year-old woman developed palpitations and presyncopes during the post-partum period after her second pregnancy. Palpitations remained unclear until a self-recorded single-lead smartwatch ECG revealed a complete episode of a fast broad complex tachycardia (260 b.p.m.) that led to hospital admission. Echocardiography, cardiac magnetic resonance imaging, and exercise testing, showed no relevant abnormalities. Recording the tachycardia in a 12-lead-ECG could eventually be achieved revealing an inferior axis and positive concordance in the precordial leads. Episodes of ventricular tachycardia (VT) could be provoked by breast feeding and mental stress, but not induced in two electrophysiological studies. Genetic testing was normal. The patient continued to experience repeated, self-terminating VT episodes, lasting between 10 and 40 s, leading to presyncopes and a syncope with a fall. The beginning of symptoms subsequent to child birth and frequent premature ventricular contractions in her first pregnancy made hormone-induced arrhythmia a tentative diagnosis. Heart rate-corrected QT (QTc) intervals showed significant variability corresponding to the frequency of episodes in a retrospective evaluation. The cessation of breastfeeding led to a termination of arrhythmias. The patient was temporarily equipped with a wearable cardioverter defibrillator vest, an implantable cardioverter defibrillator (ICD) was not implanted.

Discussion

The case report highlights the potential of self-recorded, patient-activated ECG monitoring in diagnosing recurrent palpitations, and the dilemma of timing for implanting ICDs in young patients with ventricular arrythmias (VTs). Additionally, it underlines the role of post-partum hormones in the susceptibility to ventricular arrhythmias, calling for further research of gender-specific, and pregnancy-associated arrhythmias.
The CNIC-polypill (acetylsalicylic acid, atorvastatin, and ramipril), an effective and cost-saving secondary prevention strategy compared with other therapeutic options in patients with ischaemic heart disease
Authors: Dalmau Regina , Cordero Alberto , Masana Luís , Ruiz Emilio , Sicras-Mainar Antoni , González-Juanatey José R
Published: April 2024

Aims

The retrospective NEPTUNO study evaluated the effectiveness of the Centro Nacional de Investigaciones Cardiovasculares (CNIC)-polypill (including acetylsalicylic acid, ramipril, and atorvastatin) vs. other therapeutic approaches in secondary prevention for cardiovascular (CV) disease. In this substudy, the focus was on the subgroup of patients with ischaemic heart disease (IHD).

Methods and results

Patients on four strategies: CNIC-polypill, its monocomponents as loose medications, equipotent medications, and other therapies. The primary endpoint was the incidence of recurrent major adverse CV events (MACEs) after 2 years. After matching, 1080 patients were included in each cohort. The CNIC-polypill cohort had a significantly lower incidence of recurrent MACE compared with monocomponents, equipotent drugs, and other therapies cohorts (16.1 vs. 24, 24.4, and 24.3%, respectively; P < 0.001). The hazard ratios (HRs) for recurrent MACE were higher in monocomponents (HR = 1.12; P = 0.042), equipotent drugs (HR = 1.14; P = 0.031), and other therapies cohorts (HR = 1.17; P = 0.016) compared with the CNIC-polypill, with a number needed to treat of 12 patients to prevent a MACE. The CNIC-polypill demonstrated a greater reduction in LDL cholesterol (LDL-c; −56.1 vs. −43.6, −33.3, and −33.2% in the monocomponents, equipotent drugs, and other therapies, respectively; P < 0.001) and systolic blood pressure (−13.7 vs. −11.5, −10.6, and −9.1% in the CNIC-polypill, monocomponents, equipotent drugs, and other therapies, respectively; P < 0.001) compared with other cohorts. The CNIC-polypill intervention was less costly and more effective than any other therapeutic option, with €2317–€2407 cost savings per event prevented.

Conclusion

In IHD, the CNIC-polypill exemplifies a guideline-recommended secondary prevention treatment linked to better outcomes and cost saving compared with other therapeutic options.
A multi-million-year natural experiment
Authors: Rivas-González Iker , Tung Jenny
Published: April 2024

Improving the diversity and quality of genome assemblies for non-human mammals has been a long-standing goal of comparative genomics. The last year saw substantial progress towards this goal, including the release of genome alignments for 240 mammals and nearly half the primate order. These resources have increased our ability to identify evolutionarily constrained regions of the genome, and together strongly support the importance of these regions to biomedically relevant trait variation in humans. They also provide new strategies for identifying the genetic basis of changes unique to individual lineages, illustrating the value of evolutionary comparative approaches for understanding human health.
Effects of Cadmium Stress on Seed Germination and Physiology and Biochemistry during Early Seedling Growth of Masson Pine (Pinus massoniana Lamb.)
Authors: Wang Yi , Qian Jing , Zhang Chi , Zhang Zhiyuan
Published: April 2024

This study evaluated the effects of cadmium (Cd) on seed germination and the physiological and biochemical characteristics of Pinus massoniana, which was used as the experimental material; culture dishes and germination boxes were used as testing devices. Seed germination and seedling growth in the presence of various Cd concentrations (0.4, 0.8, 1.2, 2, and 0 mg/L) were explored. The germination indexes of Pinus massoniana seeds used were germination percentage (GP), germination energy (GE), germination index (GI), vigor index (VI). and embryo length. The indexes of early growth of seedlings used were soluble sugar and soluble protein content, antioxidant enzyme activity, Cd content, and Cd enrichment. We found that Pinus massoniana presented reduced GP, GE, GI, VI, and embryo length with increasing Cd concentrations, and the VI and embryo length significantly decreased (P < .05). The physiological and biochemical indexes of Pinus massoniana seedlings were promoted at low Cd concentrations and inhibited at high Cd concentrations; each index peaked under 0.4 mg/L Cd. The Cd content in seedlings and the degree of enrichment decreased and then increased with increasing Cd concentration. Grey correlation analysis results showed that the effects of Cd stress were the strongest on embryo length in seeds (correlation degree: 0.769).

Study Implications: The effect of heavy metal pollution on seed germination and growth is closely related to phytoremediation of heavy metal soil. Pinus massoniana is the most widely distributed pine species in China. It is an economically important industrial timber with important ecological value. This study found that under the stress of different cadmium concentrations, heavy metal Cd stress inhibited the germination of Pinus massoniana seeds, and moderate Cd concentration promoted the physiological and biochemical of Pinus massoniana seedlings. Pinus massoniana seedlings have the ability to enrich heavy metal Cd.
“Not everything that can be counted counts” in ethanol toxicological results: an antemortem and postmortem technical interpretation focusing on driving under the influence
Authors: Dinis-Oliveira Ricardo Jorge
Published: April 2024

Ethanol blood analysis is the most common request in forensic toxicology, and some studies point to positive results in approximately one-third of all unnatural deaths. However, distinguishing sober deaths from drunk deaths is not as simple as it may seem. This technical, clinical, and forensic interpretation is proposed to interpret the ethanol toxicological results, discussing several artefacts and pitfalls that must be considered, namely focusing on driving under the influence. This work is presented with a practical and objective approach, aiming to alleviate the complexities associated with clinical, physiological, pathophysiological, and toxicological aspects to enhance comprehension, practicality, and applicability of its content, especially to courts. Particularly the physical integrity of the body, the postmortem interval, putrefactive signs, anatomic place of blood collection, alternative samples such as vitreous humour and urine, the possibility of postmortem redistribution, the inclusion of preservatives in containers, and optimal temperature conditions of shipment are among some of the aspects to pay attention. Although several biomarkers related to postmortem microbial ethanol production have been proposed, their translation into forensic routine is slow to be implemented due to the uncertainties of their application and analytical difficulties. Specifically, in the interpretation of ethanol toxicological results, “not everything that can be counted counts and not everything that counts can be counted” (attributed to Albert Einstein).
Improved reference quality genome sequence of the plastic-degrading greater wax moth, Galleria mellonella
Authors: Young Reginald , Ahmed Khandaker Asif , Court Leon , Castro-Vargas Cynthia , Marcora Anna , Boctor Joseph , Paull Cate , Wijffels Gene , Rane Rahul , Edwards Owain , Walsh Tom , Pandey Gunjan
Published: April 2024

Galleria mellonella is a pest of honeybees in many countries because its larvae feed on beeswax. However, G. mellonella larvae can also eat various plastics, including polyethylene, polystyrene, and polypropylene, and therefore, the species is garnering increasing interest as a tool for plastic biodegradation research. This paper presents an improved genome (99.3% completed lepidoptera_odb10 BUSCO; genome mode) for G. mellonella. This 472 Mb genome is in 221 contigs with an N50 of 6.4 Mb and contains 13,604 protein-coding genes. Genes that code for known and putative polyethylene-degrading enzymes and their similarity to proteins found in other Lepidoptera are highlighted. An analysis of secretory proteins more likely to be involved in the plastic catabolic process has also been carried out.
Social Activities and Risk of Dementia in Community-Dwelling Older People: Gender-Specific Findings From a Prospective Cohort Study
Authors: Htun Htet Lin , Teshale Achamyeleh Birhanu , Owen Alice J , Ryan Joanne , Woods Robyn L , Orchard Suzanne G , Hajek André , Lysen Thom , Shah Raj C , Chong Trevor T-J , Sheets Kerry M , Joyce Johanna , Murray Anne M , Freak-Poli Rosanne
Published: April 2024

Objectives

This study examines the gender-specific associations between a wide range of social activities and dementia risk.

Methods

A prospective cohort study was conducted involving community-dwelling older Australians (≥70 years) without significant cognitive impairment at enrolment. During the first year of enrolment, we assessed 25 self-reported social activities covering various aspects, including support from relatives and friends, community participation, social interactions with surroundings, and loneliness. Dementia diagnosis followed DSM-IV criteria, adjudicated by an international expert panel. To estimate hazard ratios (HR) and 95% confidence intervals (CI) for associations between social activities and dementia, we performed Cox proportional hazards models, adjusting for age, educational attainment, baseline global cognition, and depressive symptoms.

Results

Among 9,936 participants who completed all social activity questionnaires (median [IQR] age: 73.4 [71.6–77.1] years; 47.4% men), dementia was diagnosed in 3.8% of men (n = 181/4,705) and 2.6% of women (n = 138/5,231) over a median 6.4 years (IQR: 5.3–7.6, range: 0.2–10.1) follow-up. Gender-specific relationships emerged: caregiving for a person with illness/disability in women (HR: 0.65, 95% CI: 0.42–0.99), and having ≥9 relatives feeling close to call for help in men (HR: 0.56, 95% CI: 0.33–0.96; reference <9 relatives) were associated with reduced dementia risk. Unexpectedly, in women, having ≥5 friends with whom they felt comfortable discussing private matters were associated with a greater dementia risk (HR: 1.69, 95% CI: 1.10–2.59; reference ≤2 friends). Imputed models further identified that babysitting/childminding was associated with lower dementia risk in men (HR: 0.75, 95% CI: 0.56–0.99). No other social activities showed significant associations with dementia.

Discussion

This study provides evidence of social activities influencing dementia risk. Further investigations are required to uncover the mechanisms driving these observed relationships.
EryDB: A Transcriptomic Profile Database for Erythropoiesis and Erythroid-related Diseases
Authors: Zheng Guangmin , Wu Song , Zhang Zhaojun , Xin Zijuan , Zhang Lijuan , Zhao Siqi , Wu Jing , Liu Yanxia , Li Meng , Ruan Xiuyan , Qiao Nan , Bao Yiming , Qu Hongzhu , Fang Xiangdong
Published: April 2024

Erythropoiesis is a finely regulated and complex process that involves multiple transformations from hematopoietic stem cells to mature red blood cells at hematopoietic sites from the embryonic to the adult stages. Investigations into its molecular mechanisms have generated a wealth of expression data, including bulk and single-cell RNA sequencing data. A comprehensively integrated and well-curated erythropoiesis-specific database will greatly facilitate the mining of gene expression data and enable large-scale research of erythropoiesis and erythroid-related diseases. Here, we present EryDB, an open-access and comprehensive database dedicated to the collection, integration, analysis, and visualization of transcriptomic data for erythropoiesis and erythroid-related diseases. Currently, the database includes expertly curated quality-assured data of 3803 samples and 1,187,119 single cells derived from 107 public studies of three species (Homo sapiens, Mus musculus, and Danio rerio), nine tissue types, and five diseases. EryDB provides users with the ability to not only browse the molecular features of erythropoiesis between tissues and species, but also perform computational analyses of single-cell and bulk RNA sequencing data, thus serving as a convenient platform for customized queries and analyses. EryDB v1.0 is freely accessible at https://ngdc.cncb.ac.cn/EryDB/home.
The Time to Inflammatory Bowel Disease Diagnosis for Patients Presenting with Abdominal Symptoms in Primary Care and its Association with Emergency Hospital Admissions and Surgery: A Retrospective Cohort Study
Authors: Umar Nosheen , Harvey Phil , Adderley Nicola J , Haroon Shamil , Trudgill Nigel
Published: April 2024

Background

Patients with inflammatory bowel disease (IBD) presenting to primary care may experience diagnostic delays. We aimed to evaluate this and assess whether time to diagnosis is associated with clinical outcomes.

Methods

A retrospective cohort study using English primary care data from January 1, 2010, to December 31, 2019, linked to hospital admission data was undertaken. Patients were followed from the first IBD-related presentation in primary care to IBD diagnosis. Associations of time to diagnosis exceeding a year were assessed using a Robust Poisson regression model. Associations between time to diagnosis and IBD-related emergency hospital admissions and surgery were assessed using Poisson and Cox proportional hazards models, respectively.

Results

Of 28 092 IBD patients, 60% had ulcerative colitis (UC) and 40% had Crohn’s disease (CD). The median age was 43 (interquartile range, 30-58) years and 51.9% were female. Median time to diagnosis was 15.6 (interquartile range, 4.3-28.1) months. Factors associated with more than a year to diagnosis included female sex (adjusted risk ratio [aRR], 1.23; 95% CI, 1.21-1.26), older age (aRR, 1.05; 95% CI, 1.01-1.10; comparing >70 years of age with 18-30 years of age), obesity (aRR, 1.03; 95% CI, 1.00-1.06), smoking (aRR, 1.05; 95% CI, 1.02-1.08), CD compared with UC (aRR, 1.13; 95% CI, 1.11-1.16), and a fecal calprotectin over 500 μg/g (aRR, 0.89; 95% CI, 0.82-0.95). The highest quartile of time to diagnosis compared with the lowest was associated with IBD-related emergency admissions (incidence rate ratio, 1.06; 95% CI, 1.01-1.11).

Conclusion

Longer times to IBD diagnoses were associated with being female, advanced age, obesity, smoking, and Crohn’s disease. More IBD-related emergency admissions were observed in patients with a prolonged time to diagnosis.
Three-dimensional printed pure-titanium implantation for chest wall reconstruction involving the sternum and ribs: a novel approach
Authors: Yoon Dong Woog , Kim Tae Ho , Cha Min Jae , Kim Gun-Hee , Lee ByoungSoo , Kim Hyung Giun , Kwon Minhyeok , Jang Donghoon , Park Byungjoon
Published: April 2024

Chest wall reconstruction is challenging due to the complex shape and large defect size. The three-dimensional printing technology enables the fabrication of customized implants, and 3D-printed pure-titanium could provide superior mechanical properties to conventional materials. The aim of this study was to evaluate long-term outcomes of patients undergoing chest wall reconstruction with a 3D-printed pure-titanium implant. Between August 2018 and May 2021, 5 patients underwent surgery due to sternal metastasis (n = 3), postoperative sternal wound infection (n = 1) and deformity (n = 1). The customized implant was designed and constructed based on the size and shape of the chest wall defect measured on computed tomography. All patients demonstrated uneventful recovery without complications during the hospital course. During the median follow-up of 20 months, 1 patient underwent revision surgery due to implant breakage, and 1 removed the implant due to trauma-related chest wall infection. One patient died from cancer progression, while 3 patients are alive without any implant-related complications. Chest wall reconstruction using a 3D-printed pure-titanium implant could be a novel alternative for patients with various conditions affecting the sternum and ribs.
Minimal Diffeomorphisms with L1 Hopf Differentials
Authors: Sagman Nathaniel
Published: April 2024

We prove that for any two Riemannian metrics $\sigma _{1}, \sigma _{2}$ on the unit disk, a homeomorphism $\partial \mathbb{D}\to \partial \mathbb{D}$ extends to at most one quasiconformal minimal diffeomorphism $(\mathbb{D},\sigma _{1})\to (\mathbb{D},\sigma _{2})$ with $L^{1}$ Hopf differential. For minimal Lagrangian diffeomorphisms between hyperbolic disks, the result is known, but this is the first proof that does not use anti-de Sitter geometry. We show that the result fails without the $L^{1}$ assumption in variable curvature. The key input for our proof is the uniqueness of solutions for a certain Plateau problem in a product of trees.
Intracellular acidification and glycolysis modulate inflammatory pathway in senescent cells
Authors: Kawakami Satoshi , Johmura Yoshikazu , Nakanishi Makoto
Published: April 2024

Senescent cells accumulate in various organs with ageing, and its accumulation induces chronic inflammation and age-related physiological dysfunctions. Several remodelling of intracellular environments have been identified in senescent cells, including enlargement of cell/nuclear size and intracellular acidification. Although these alterations of intracellular environments were reported to be involved in the unique characteristics of senescent cells, the contribution of intracellular acidification to senescence-associated cellular phenotypes is poorly understood. Here, we identified that the upregulation of TXNIP and its paralog ARRDC4 as a hallmark of intracellular acidification in addition to KGA-type GLS1. These genes were also upregulated in response to senescence-associated intracellular acidification. Neutralization of the intracellular acidic environment ameliorated not only senescence-related upregulation of TXNIP, ARRDC4 and KGA but also inflammation-related genes, possibly through suppression of PDK-dependent anaerobic glycolysis. Furthermore, we found that expression of the intracellular acidification-induced genes, TXNIP and ARRDC4, correlated with inflammatory gene expression in heterogeneous senescent cell population in vitro and even in vivo, implying that the contribution of intracellular pH to senescence-associated cellular features, such as SASP.
Muscle density, but not size, is independently associated with cognitive health in older adults with hip fractures
Authors: Ge Yufeng , You Qian , Gao Feng , Liu Gang , Wang Ling , Li Bo , Tian Maoyi , Yang Minghui , Wu Xinbao
Published: April 2024

Emerging evidence indicates a complex interplay between skeletal muscle and cognitive function. Despite the known differences between muscle quantity and quality, which can be measured via computed tomography (CT), the precise nature of their associations with cognitive performance remain underexplored. To investigate the links between muscle size and density and cognitive impairment (CI) in the older adults with hip fractures, we conducted a post hoc, cross-sectional analysis within a prospective cohort study on 679 patients with hip fractures over 65. Mini-Mental State Examination (MMSE) and routine hip CT imaging were utilized to assess cognition function and muscle characteristics in older adults with hip fractures. The CT scans provided data on cross-sectional area and attenuation for the gluteus maximus (G.MaxM) and the combined gluteus medius and minimus (G.Med/MinM). Participants were categorized into CI and non-CI groups based on education levels and MMSE scores. Multivariate logistic regressions, propensity score (PS) methods, and subgroup analysis were employed to analyze associations and validate findings. This study included 123 participants (81.6 ± 6.8 years, 74% female) with CI and 556 participants (78.5 ± 7.7 years, 72% female) without. Compared to the non-CI group, muscle parameters, especially density, were significantly lower in the CI group. Specifically, G.Med/Min muscle density, but not size was robustly associated with CI (odds ratio (OR) = 0.77, 95% confidence interval = 0.62–0.96, P = 0.02), independent of other medical situations. Sensitivity analysis corroborated that G.Med/Min muscle density was consistently lower in the CI group than the non-CI group, as evidenced in the PS matched (P = 0.024) and weighted cohort (P = 0.033). Enhanced muscle parameters, particularly muscle density in the G.Med/MinM muscle, correlate with a lower risk of CI. Muscle density demonstrates a stronger association with cognitive performance than muscle size, highlighting its potential as a key focus in future cognitive health research.
Pigeon pea crop stage strongly influences plant susceptibility to Helicoverpa armigera (Lepidoptera: Noctuidae)
Authors: Volp Trevor M , Zalucki Myron P , Furlong Michael J
Published: April 2024

Helicoverpa armigera Hübner (Lepidoptera: Noctuidae; Hübner) is the major insect pest of pigeon pea [Cajanus cajan; Fabales: Fabaceae; (L.) Millspaugh] worldwide. Research to develop pest management strategies for H. armigera in pigeon pea has focused heavily on developing less susceptible cultivars, with limited practical success. We examined how pigeon pea crop stage influences plant susceptibility to H. armigera using a combination of glasshouse and laboratory experiments. Plant phenology significantly affected oviposition with moths laying more eggs on flowering and podding plants but only a few on vegetative plants. Larval survival was greatest on flowering and vegetative plants, wherein larvae mostly chose to feed inside flowers on flowering plants and on the adaxial surface of expanding leaves on vegetative plants. Larval survival was poor on podding plants despite moths laying many eggs on plants of this stage. When left to feed without restriction on plants for 7 days, larvae feeding on flowering plants were >10 times the weight of larvae feeding on plants of other phenological stages. On whole plants, unrestricted larvae preferred to feed on pigeon pea flowers and on expanding leaves, but in no-choice Petri dish assays H. armigera larvae could feed and survive on all pigeon pea reproductive structures. Our results show that crop stage and the availability of flowers strongly influence pigeon pea susceptibility to H. armigera. An increased understanding of H. armigera-pigeon pea ecology will be useful in guiding the development of resistant varieties and other management tactics.
A large cheek lipoma misdiagnosed as a dental infection: a rare case report
Authors: Hamamin Omed Shafiq , Abdulrahman Saman Wahid , Abdul Aziz Jeza M , Rashid Muhammad Jabar , Rasheed Mariwan Kadir , Najmalddin Lana Dara , Ahmad Kani Abdulrahman , Mohammed Baz Bakhtyar , Sleman Nida Khalid , Huy Nguyen Tien
Published: April 2024

The most frequent benign tumor is lipoma. About 1–4% of people have intraoral lipomas. This uncommon case report shows a large cheek lipoma misinterpreted as dental infection. A 14-year-old girl with a right cheek tumor was diagnosed and treated for a dental infection. Multiple imaging examinations complete the diagnosis. A benign lipoma, common in subcutaneous tissues but unusual in the mouth, created the lesion. A histological investigation confirmed lipoma after extraoral excision under general anesthesia utilizing an external flap. The edema did not return after surgery, which restored face symmetry. This case study shows that diagnosing and treating oral and maxillofacial edema requires extensive clinical and radiographic testing. Lipomas should be evaluated in the differential diagnosis of mouth swelling even without usual risk markers to prevent excessive treatments and delays. Surgery is recommended for lipomas since it has a low recurrence and fewer risks.
Management of ossifying fibroma of the bone of the maxilla: a case report and review of the literature
Authors: Nnko Kanankira A , Pima Raphael T , Damas Sosthenes , Mremi Alex
Published: April 2024

Ossifying fibroma (OF) is a slow-growing benign fibro-osseous neoplasm. It is mostly odontogenic in origin, and it arises in the jaws, particularly the mandible. OF is characterized by the production of bone and cementum-like calcifications in a fibrous stroma. OF reports of the bone of the maxilla are uncommon. Diagnosing OF can be challenging due to the considerable overlap of clinico-pathological characteristics with those of other neoplasms. Herein, the authors describe a case report OF in a 26-year-old male who presented with a huge fibro-osseous tumor of the maxilla. Histopathology established the diagnosis of maxillary OF. The tumor was surgically removed via a Weber–erguson approach with satisfactory functional and cosmetic results. No recurrence has been found after one year of follow-up. Clinical, radiological, and pathological characteristics, as well as surgical treatment approaches, are further discussed. This is one of a few documented cases of maxillary OF in our setting.
WFC3 Infrared Spectroscopic Parallel (WISP) survey: photometric and emission-line data release
Authors: Battisti A J , Bagley M B , Rafelski M , Baronchelli I , Dai Y S , Henry A L , Atek H , Colbert J , Malkan M A , McCarthy P J , Scarlata C , Siana B , Teplitz H I , Alavi A , Boyett K , Bunker A J , Gardner J P , Hathi N P , Masters D , Mehta V , Rutkowski M , Shahinyan K , Sunnquist B , Wang X
Published: April 2024

We present reduced images and catalogues of photometric and emission-line data (∼230 000 and ∼8000 sources, respectively) for the WFC3 (Wide Field Camera 3) Infrared Spectroscopic Parallel (WISP) survey. These data are made publicly available on the Mikulski Archive for Space Telescopes and include reduced images from various facilities: ground-based ugri, Hubble Space Telescope (HST) WFC3, and Spitzer IRAC (Infrared Array Camera). Coverage in at least one additional filter beyond the WFC3/IR data are available for roughly half of the fields (227 out of 483), with ∼20 per cent (86) having coverage in six or more filters from u band to IRAC 3.6 $\mu$m (0.35–3.6 $\mu$m). For the lower spatial resolution (and shallower) ground-based and IRAC data, we perform PSF (point spread function)-matched, prior-based, deconfusion photometry (i.e. forced-photometry) using the tphot software to optimally extract measurements or upper limits. We present the methodology and software used for the WISP emission-line detection and visual inspection. The former adopts a continuous wavelet transformation that significantly reduces the number of spurious sources as candidates before the visual inspection stage. We combine both WISP catalogues and perform spectral energy distribution fitting on galaxies with reliable spectroscopic redshifts and multiband photometry to measure their stellar masses. We stack WISP spectra as functions of stellar mass and redshift and measure average emission-line fluxes and ratios. We find that WISP emission-line sources are typically ‘normal’ star-forming galaxies based on the mass–excitation diagram ([O iii]/Hβ versus M⋆; 0.74 < zgrism < 2.31), the galaxy main sequence (SFR versus M⋆; 0.30 < zgrism < 1.45), S32 ratio versus M⋆ (0.30 < zgrism < 0.73), and O32 and R23 ratios versus M⋆ (1.27 < zgrism < 1.45).
Robust inference of the Galactic Centre gamma-ray excess spatial properties
Authors: Song Deheng , Eckner Christopher , Gordon Chris , Calore Francesca , Macias Oscar , Abazajian Kevork N , Horiuchi Shunsaku , Kaplinghat Manoj , Pohl Martin
Published: April 2024

The gamma-ray Fermi-LAT Galactic Centre excess (GCE) has puzzled scientists for over 15 yr. Despite ongoing debates about its properties, and especially its spatial distribution, its nature remains elusive. We scrutinize how the estimated spatial morphology of this excess depends on models for the Galactic diffuse emission, focusing particularly on the extent to which the Galactic plane and point sources are masked. Our main aim is to compare a spherically symmetric morphology – potentially arising from the annihilation of dark matter (DM) particles – with a boxy morphology – expected if faint unresolved sources in the Galactic bulge dominate the excess emission. Recent claims favouring a DM-motivated template for the GCE are shown to rely on a specific Galactic bulge template, which performs worse than other templates for the Galactic bulge. We find that a non-parametric model of the Galactic bulge derived from the VISTA Variables in the Via Lactea survey results in a significantly better fit for the GCE than DM-motivated templates. This result is independent of whether a galprop-based model or a more non-parametric ring-based model is used to describe the diffuse Galactic emission. This conclusion remains true even when additional freedom is added in the background models, allowing for non-parametric modulation of the model components and substantially improving the fit quality. When adopted, optimized background models provide robust results in terms of preference for a boxy bulge morphology for the GCE, regardless of the mask applied to the Galactic plane.
Risk factors for serious infections and infection-related mortality in patients with microscopic polyangiitis: Multicentre REVEAL cohort study
Authors: Manabe Atsushi , Kadoba Keiichiro , Hiwa Ryosuke , Kotani Takuya , Shoji Mikihito , Shirakashi Mirei , Tsuji Hideaki , Kitagori Koji , Akizuki Shuji , Nakashima Ran , Yoshifuji Hajime , Yamamoto Wataru , Okazaki Ayana , Matsuda Shogo , Gon Takaho , Watanabe Ryu , Hashimoto Motomu , Morinobu Akio
Published: April 2024

Objective

Infections are a critical concern for patients with microscopic polyangiitis (MPA). This study aimed to identify the risk factors associated with serious infections (SIs) and infection-related mortality in patients with MPA, as well as the effect of glucocorticoid (GC) dose tapering on these outcomes.

Methods

This multicentre, retrospective, and observational study utilised data from a cohort of patients with MPA in Japan [Registry of Vasculitis Patients to Establish REAL World Evidence (REVEAL) cohort]. Patients were categorised based on the occurrence of SIs or infection-related deaths, and various characteristics were compared among the groups.

Results

Among 182 patients, 66 (36.2%) experienced 129 SIs and 27 (14.8%) developed infection-related deaths. Advanced age, elevated C-reactive protein (CRP) levels, and higher ratio of the GC dose at 3 months to the initial dose were identified as independent risk factors for SIs. Older age was also associated with infection-related deaths. Furthermore, the cumulative incidence of infection-related deaths was significantly higher in patients with a higher ratio of the GC dose at 24 months to the initial dose.

Conclusion

Older age, elevated CRP levels, and slower GC dose tapering predispose patients to SIs and infection-related deaths. Strategies, such as rapid GC dose tapering, are anticipated to mitigate the risk of infections.
Circulating extracellular vesicles as biomarker for diagnosis, prognosis, and monitoring in glioblastoma patients
Authors: Ricklefs Franz L , Wollmann Kathrin , Salviano-Silva Amanda , Drexler Richard , Maire Cecile L , Kaul Michael G , Reimer Rudolph , Schüller Ulrich , Heinemann Sarina , Kolbe Katharina , Mummert Tobias , Glatzel Markus , Peine Sven , Gempt Jens , Westphal Manfred , Dührsen Lasse , Lamszus Katrin
Published: April 2024

Background

Extracellular vesicles (EVs) obtained by noninvasive liquid biopsy from patient blood can serve as biomarkers. Here, we investigated the potential of circulating plasma EVs to serve as an indicator in the diagnosis, prognosis, and treatment response of glioblastoma patients.

Methods

Plasma samples were collected from glioblastoma patients at multiple timepoints before and after surgery. EV concentrations were measured by nanoparticle tracking analysis and imaging flow cytometry. Tumor burden and edema were quantified by 3D reconstruction. EVs and tumors were further monitored in glioma-bearing mice.

Results

Glioblastoma patients displayed a 5.5-fold increase in circulating EVs compared to healthy donors (P < .0001). Patients with higher EV levels had significantly shorter overall survival and progression-free survival than patients with lower levels, and the plasma EV concentration was an independent prognostic parameter for overall survival. EV levels correlated with the extent of peritumoral fluid-attenuated inversion recovery hyperintensity but not with the size of the contrast-enhancing tumor, and similar findings were obtained in mice. Postoperatively, EV concentrations decreased rapidly back to normal levels, and the magnitude of the decline was associated with the extent of tumor resection. EV levels remained low during stable disease, but increased again upon tumor recurrence. In some patients, EV resurgence preceded the magnetic resonance imaging detectability of tumor relapse.

Conclusions

Our findings suggest that leakiness of the blood-brain barrier may primarily be responsible for the high circulating EV concentrations in glioblastoma patients. Elevated EVs reflect tumor presence, and their quantification may thus be valuable in assessing disease activity.
Preoperative growth dynamics of untreated glioblastoma: Description of an exponential growth type, correlating factors, and association with postoperative survival
Authors: Feucht Daniel , Haas Patrick , Skardelly Marco , Behling Felix , Rieger David , Bombach Paula , Paulsen Frank , Hoffmann Elgin , Hauser Till-Karsten , Bender Benjamin , Renovanz Mirjam , Niyazi Maximilian , Tabatabai Ghazaleh , Tatagiba Marcos , Roder Constantin
Published: April 2024

Background

Little is known about the growth dynamics of untreated glioblastoma and its possible influence on postoperative survival. Our aim was to analyze a possible association of preoperative growth dynamics with postoperative survival.

Methods

We performed a retrospective analysis of all adult patients surgically treated for newly diagnosed glioblastoma at our center between 2010 and 2020. By volumetric analysis of data of patients with availability of ≥3 preoperative sequential MRI, a growth pattern was aimed to be identified. Main inclusion criterion for further analysis was the availability of two preoperative MRI scans with a slice thickness of 1 mm, at least 7 days apart. Individual growth rates were calculated. Association with overall survival (OS) was examined by multivariable.

Results

Out of 749 patients screened, 13 had ≥3 preoperative MRI, 70 had 2 MRI and met the inclusion criteria. A curve estimation regression model showed the best fit for exponential tumor growth. Median tumor volume doubling time (VDT) was 31 days, median specific growth rate (SGR) was 2.2% growth per day. SGR showed negative correlation with tumor size (rho = −0.59, P < .001). Growth rates were dichotomized according to the median SGR.OS was significantly longer in the group with slow growth (log-rank: P = .010). Slower preoperative growth was independently associated with longer overall survival in a multivariable Cox regression model for patients after tumor resection.

Conclusions

Especially small lesions suggestive of glioblastoma showed exponential tumor growth with variable growth rates and a median VDT of 31 days. SGR was significantly associated with OS in patients with tumor resection in our sample.
Effects of group size on movement patterns and clustering dynamics in rats
Authors: Monfils Marie-H , Pasala Michael , Malone Cassidy , Agee Laura , Roquet Rheall , Cormack Lawrence
Published: April 2024

Environment is a determining factor that can facilitate or hinder social interactions. A precursor to meaningfully engaging with conspecifics is being exposed to opportunistic encounters with others. Increasing the number of individuals in a given space (thus increasing density) would, statistically speaking, increase the likelihood of accidental encounters. This might have consequences on the formation of social networks—an idea that has not reliably been explored. If true, we would expect that increasing density would lead to an increase in the number and the duration of ‘clusters’ of animals. Here, we examined whether varying the number of rats in an open field environment differentially affected their movement dynamics or their propensity to aggregate into clusters and, if so, whether such effects are dependent solely on statistical factors due to increases in density, the potential for actively-sought social interactions, or both. We found that the number of rats in an environment impacts ambulation speed, distance traveled, cluster formation and approaches, and that number and duration of clusters are highly dependent on the propensity for the rats to engage in social interactions.
A secreted bacterial protein protects bacteria from cationic antimicrobial peptides by entrapment in phase-separated droplets
Authors: Ostan Nicholas K H , Cole Gregory B , Wang Flora Zhiqi , Reichheld Sean E , Moore Gaelen , Pan Chuxi , Yu Ronghua , Lai Christine Chieh-Lin , Sharpe Simon , Lee Hyun O , Schryvers Anthony B , Moraes Trevor F
Published: April 2024

Mammalian hosts combat bacterial infections through the production of defensive cationic antimicrobial peptides (CAPs). These immune factors are capable of directly killing bacterial invaders; however, many pathogens have evolved resistance evasion mechanisms such as cell surface modification, CAP sequestration, degradation, or efflux. We have discovered that several pathogenic and commensal proteobacteria, including the urgent human threat Neisseria gonorrhoeae, secrete a protein (lactoferrin-binding protein B, LbpB) that contains a low-complexity anionic domain capable of inhibiting the antimicrobial activity of host CAPs. This study focuses on a cattle pathogen, Moraxella bovis, that expresses the largest anionic domain of the LbpB homologs. We used an exhaustive biophysical approach employing circular dichroism, biolayer interferometry, cross-linking mass spectrometry, microscopy, size-exclusion chromatography with multi-angle light scattering coupled to small-angle X-ray scattering (SEC–MALS-SAXS), and NMR to understand the mechanisms of LbpB-mediated protection against CAPs. We found that the anionic domain of this LbpB displays an α-helical secondary structure but lacks a rigid tertiary fold. The addition of antimicrobial peptides derived from lactoferrin (i.e. lactoferricin) to the anionic domain of LbpB or full-length LbpB results in the formation of phase-separated droplets of LbpB together with the antimicrobial peptides. The droplets displayed a low rate of diffusion, suggesting that CAPs become trapped inside and are no longer able to kill bacteria. Our data suggest that pathogens, like M. bovis, leverage anionic intrinsically disordered domains for the broad recognition and neutralization of antimicrobials via the formation of biomolecular condensates.