bluuebunny/crossref_metadata_2025 huggingface dataset:
N-Carbamoylglutamate Supplementation on the Digestibility, Rumen Fermentation, Milk Quality, Antioxidant Parameters, and Metabolites of Jersey Cattle in High-Altitude Areas
Authors: Liu Zixin , Yan Fuyong , Mi Hui , Lv Xiaokang , Wang Kaijun , Li Bin , Jin Tao , Chen Liang , Zhang Guijie , Huang Ximei , Zhou Chuanshe , Tan Zhiliang
Published: April 2022

This study aimed to assess the impact of the dietary supplementation of N-carbamoylglutamate (NCG) on nutrient digestibility, rumen fermentation, milk quality, oxidative stress, and metabolites in the plasma and feces of Jersey cattle under high altitude with the hypoxic condition. A total of 14 healthy lactating Jersey dairy cows with similar body conditions were selected and randomly divided into 2 groups. The control group (CON group, N = 6 replicates) was fed with a conventional complete diet, whereas the experimental group (NCG group, N = 8 replicates) received 20 g/d per head NCG supplementation. The experiment lasted for 60 days, the adaptation period was 12 days, and the formal experiment period was 48 days. Except that the NCG group showed an upward trend in dry matter intake (DMI) (p = 0.09) and the fermentation parameters, the molar proportion of butyric acid tended to decrease (p = 0.08); the two groups had no significant differences (p > 0.05) in nutrients digestibility, plasma immunity, and antioxidant ability. However, compared with the CON group, the milk fat rate and blood oxygen saturation of the NCG group showed an upward trend (p = 0.09). For indexes associated with altitude stress, the contents of thyroxine, transferrin, and endothelin both decreased significantly (p < 0.05) in the NCG group. Meanwhile, heat shock protein (p = 0.07) and aldosterone (p = 0.06) also showed a downward trend. A total of 114 different metabolites were identified from feces and plasma, 42 metabolites were derived from plasma that mainly included 5 kinds of Super Class, and 72 metabolites were derived from feces that mainly included 9 kinds of Super Class. The significantly increased plasma differential metabolites were 2,5-dihydroxybenzoate and salicyluric acid, and the significantly increased fecal differential metabolites were Butenafine (fold change > 2). Pathway analysis showed that after applying NCG as a feed additive, the changes of the Jersey dairy cows mainly focused on amino acid metabolism and lipid metabolism. These results indicated that adding NCG to the diet can prevent the hypoxic stress state of lactating Jersey cows in high-altitude areas and has a tendency to improve milk quality.
Lipoamide Alleviates Oxidized Fish Oil-Induced Host Inflammatory Response and Oxidative Damage in the Oviduct of Laying Hens
Authors: Liu Qingxiu , Li Wenxiang , Zhang Jiatu , Zhao Lihong , Ji Cheng , Zhang Jianyun , Huang Shimeng , Ma Qiugang
Published: April 2022

Fish oil (FO) is an important source of lipid in functional food and aquafeeds. However, the harmful effects of oxidized fish oil (OFO) on host metabolism and reproductive health are not yet clear. In addition, lipoamide (LAM) has been widely studied as an agent for alleviating various diseases associated with oxidative disruption. Therefore, in the current study, to investigate the effects of LAM in alleviating OFO-induced decline in reproductive performance and oxidative damage to the oviduct in laying hens. We constructed a 1% fresh FO model, a 1% OFO model, and a LAM model with 1% OFO (OFO + LAM) added at 100 mg/kg to explore the antioxidant effect of LAM. Herein, these results were evaluated by breeding performance, immune responses, estrogen, and antioxidant indices of serum samples, as well as the number of follicles and antioxidant parameters of oviducts. From the results, compared with the FO group, OFO significantly decreased the egg-laying rate, increased the contents of total protein (TP) and inflammatory factors [tumor necrosis factor α (TNF-α), interleukin (IL)-6, IL-8, and interferon γ (INF-γ)], and reduced the concentrations of anti-oxidation [total antioxidant (T-AOC), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), glutathione (GSH), glutathione reductase (GR), catalase (CAT), and hydroxyl radical scavenging activity (HRSA)] in serum samples, as well as reduced the levels of anti-oxidation indexes in oviduct tissues (p < 0.05). Of note, the supplementation of LAM could significantly increase the laying performance, improve the levels of serum immunoglobulins (IgA, IgG, and IgM), serum estrogen [progesterone (P) and estradiol (E2)], and serum antioxidant parameters (T-AOC, T-SOD, GSH-Px, GSH, GR, CAT, and HRSA) and decrease the concentrations of serum inflammatory cytokines (TNF-α, IL-6, IL-8, and INF-γ) in laying hens following OFO administration (p < 0.05). In addition, LAM could dramatically increase the contents of antioxidant factors (p < 0.05) in oviducts and enhance the secretion capacity of the uterine part. Taken together, OFO caused host metabolic dysfunction, oxidative damage, uterine morphological abnormalities, and alterations of ovarian function. These results suggested that LAM administration could alleviate host metabolic dysfunctions and inflammatory damage, and then ameliorate oxidative damage in the oviduct induced by OFO, ultimately improving reproductive function.
Trends in Donation After Circulatory Death in Lung Transplantation in the United States: Impact Of Era
Authors: Bobba Christopher M. , Whitson Bryan A. , Henn Matthew C. , Mokadam Nahush A. , Keller Brian C. , Rosenheck Justin , Ganapathi Asvin M.
Published: April 2022

Background: Use of lungs donated after circulatory death (DCD) has expanded, but changes in donor/recipient characteristics and comparison to brain dead donors (DBD) has not been studied. We examined the evolution of the use of DCD lungs for transplantation and compare outcomes to DBD lungs.

Methods: The SRTR database was used to construct three 5-year intervals. Perioperative variables and survival were compared by era and for DCD vs. DBD. Geographic variation was estimated using recipient permanent address.

Results: 728 DCD and 27,205 DBD lung transplants were identified. DCD volume increased from Era 1 (n = 73) to Era 3 (n = 528), representing 1.1% and 4.2% of lung transplants. Proportionally more DCD recipients were in ICU or on ECMO pre-transplant, and had shorter waitlist times. DCD donors were older, had lower PaO2/FiO2 ratios compared to DBD, more likely to be bilateral, had longer ischemic time, length of stay, post-op dialysis, and increased use of lung perfusion. There was no difference in overall survival. Geographically, use was heterogeneous.

Conclusion: DCD utilization is low but increasing. Despite increasing ischemic time and transplantation into sicker patients, survival is similar, which supports further DCD use in lung transplantation. DCD lung transplantation presents an opportunity to continue to expand the donor pool.
Propagation Characteristics of Modulated EHF Signal in the Wake Region of Plasma Sheath
Authors: Yang Xiaocui , Yuan Kai , Wang Yuhao , Liu Yiwen , Xiong Jiawei
Published: April 2022

A large number of studies have confirmed that the wake region may be a more ideal antenna installation area for the reentry vehicle communication problem, but many practical issues such as how to choose the modulation mode, carrier frequency, and antenna orientation are still pending. Based on numerical simulations, the characteristics of tail channels and the bit error rate (BER) of extremely high-frequency (EHF) communication in the wake region of the plasma sheath of hypersonic vehicle are studied. It is found that, with an increase in the angle between the tail channel and the tail of the vehicle, the attenuation of the EHF signals decreases and the phase shift fluctuates more severely. In order to obtain better communication performance, 2PSK with a carrier frequency of 140 GHz or 225 GHz, or 4QAM (QPSK) modulation with a carrier frequency of 140 GHz, and the tail channel with an angle between 50° and 60° to the tail of the vehicle can be selected. This study reveals the propagation characteristics and BER performance of EHF signals in the wake region of plasma sheath, which can provide a valuable reference for the design of the hypersonic vehicle communication system.
Multiple Factors at Regional, Landscape, and Local Scales Determine Spider Assemblage Composition in Pomegranate Orchards
Authors: Salman Ibrahim N. A. , Lubin Yael , Gavish-Regev Efrat
Published: April 2022

Orchards may support high spider diversity relative to annual crops due to their structural complexity and relative stability. Multiple environmental conditions at the landscape, regional, and local scales may influence the assemblage composition of spiders in orchards. We asked which environmental variables best explains spider assemblage composition and the similarity of the assemblages of pomegranate orchards located along a latitudinal gradient in Israel? Spiders were sampled from twelve pomegranate orchards along a climatic gradient from north to south. Orchards located at higher latitudes had richer assemblages than orchards located at lower latitudes. The assemblage composition was influenced at the large scale by latitude and annual crop cover in the surrounding landscape but not by local factors. By contrast, functional groups based on foraging strategies were affected by local scale factors, such as vegetation within the orchards. This study demonstrates the importance of considering environmental variables at different scales to understand the factors affecting the assemblage composition of naturally occurring predators in the orchard.
The Roles of Multidrug and Toxic Compound Extrusion (MATE) Transporters in Regulating Agronomic Traits
Authors: Ku Yee-Shan , Cheng Sau-Shan , Cheung Ming-Yan , Lam Hon-Ming
Published: April 2022

Multidrug and toxic compound extrusion (MATE) transporters are ancient proteins conserved among various kingdoms, from prokaryotes to eukaryotes. In plants, MATEs usually form a large family in the genome. Homologous MATE transporters have different subcellular localizations, substrate specificities, and responses to external stimuli for functional differentiations. The substrates of MATEs in plants include polyphenols, alkaloids, phytohormones, and ion chelators. The accumulation of these substrates is often associated with favorable agronomic traits such as seed and fruit colors, the balance between dormancy and germination, taste, and stress adaptability. In crops, wild germplasms and domesticated germplasms usually have contrasting agronomic traits such as seed color, seed taste, and stress tolerance. MATE transporters are involved in the regulations of these traits. In this review, we discuss the uniqueness and significance of there being such a large family of MATEs in plants, their substrate diversity that enables them to be involved in various agronomic traits, and the allelic forms and the expression patterns of MATE that are associated with favorable agronomic traits in domesticated crops. The understanding on the roles of MATEs in regulating favorable agronomic traits in crops will provide hints for the selection of genes for molecular breeding that improve desirable traits.
Unsupervised Driving Situation Detection in Latent Space for Autonomous Cars
Authors: Rodríguez-Hernández Erick , Vasquez Juan Irving , Duchanoy Martínez Carlos Alberto , Taud Hind
Published: April 2022

A policy determines the action that an autonomous car needs to take according to its current situation. For example, the car keeps itself on track or overtakes another car, among other policies. Some autonomous cars could need more than one policy to drive appropriately. In those systems, the behavior selector subsystem selects the policy that the car needs to follow. However, in the current literature, there is not a unified way to create these policies. In most cases, the amount and definition of the policies are hand-engineering using the information taken from observations and the knowledge of the engineers. That paradigm requires a lot of human effort. Additionally, there is human subjectivity due to the hand labeling. Furthermore, the experts could not agree about the number of existing situations and the boundaries between policies (the point at which one situation turns into another). To deal with the subjectivity of setting the number and definition of policies, we propose a novel approach that uses the “divide and conquer” paradigm. This method first, sets the number of required policies by clustering previous observations into situations, and second, it configures a regression-based policy for each situation. As a result, (i) the method can detect driving situations from raw data automatically using unsupervised algorithms, helping to avoid the hand-engineering made by an expert, and (ii) the method creates relatively small and efficient policies without human intervention using behavioral cloning. To validate the method, we have collected a custom dataset in simulation and we have conducted several experiments comparing the performance of our proposal versus two state-of-the-art end-to-end methods. Our results show that our method outperforms the end-to-end approaches in terms of a bigger R square metric (0.19 over the tested methods) and a lower mean squared error (0.48 below the tested methods).
Association between Ambient Air Pollutants and Pneumonia in Wuhan, China, 2014–2017
Authors: Zhang Huihui , Liu Suyang , Dou Qiujun , Huang Zenghui , Lv Chengcheng , Liao Jingling , Tao Fengxi , Liu Yuehua , Ma Lu
Published: April 2022

Objectives: To assess associations between short-time air pollution exposure and outpatient visits for pneumonia by the distributed lag nonlinear model (DLNM). Methods: Daily outpatient visits for pneumonia and air pollutant data were collected from Wuhan Basic Medical Insurance Database in China and 10 national air quality monitoring stations in Wuhan from 2014 to 2017, respectively. Taking the first percentile of the concentration as the reference, DLNM was used to estimate the impact of moderate (50th) and high levels (99th) of pollutants on pneumonia. Results: A total of 133,882 outpatient visits were identified during the period of the study. Moderate-level (P50) fine particulate matter (PM2.5) or sulfur dioxide (SO2) and high-level nitrogen dioxide (NO2) (P99) can increase the risk of pneumonia. The maximum RR was 1.198 (95% CI: 1.094–1.311) at lag0-11, 1.304 (95% CI: 1.166–1.458) at lag0-13, and 1.286 (95% CI: 1.060–1.561) at lag0-14, respectively. Females and children had greater risks. Conclusions: Short-time PM2.5, SO2, and NO2 exposure were associated with outpatient visits for pneumonia in Wuhan, China.
Spectral Methods for Response Enhancement of Microwave Resonant Sensors in Continuous Non-Invasive Blood Glucose Monitoring
Authors: Buonanno Giovanni , Brancaccio Adriana , Costanzo Sandra , Solimene Raffaele
Published: April 2022

In this paper, the performance of three recent algorithms for the frequency-response enhancement of microwave resonant sensors are compared. The first one, a single-step algorithm, is based on a couple of direct-inverse Fourier transforms, giving a densely sampled response as a result. The second algorithm exploits an iterative procedure to progressively restricts the frequency response. The final one is based on the super-resolution MUSIC algorithm. The comparison is carried out through a Monte Carlo analysis. In particular, synthetic signals are firstly exploited to mimic the frequency response of a resonant microwave sensor. Then, experimental data collected from water-glucose solutions are adopted as validation test for potential applications in noninvasive blood-glucose monitoring.
H2S in Critical Illness—A New Horizon for Sodium Thiosulfate?
Authors: Merz Tamara , McCook Oscar , Brucker Cosima , Waller Christiane , Calzia Enrico , Radermacher Peter , Datzmann Thomas
Published: April 2022

Ever since the discovery of endogenous H2S and the identification of its cytoprotective properties, efforts have been made to develop strategies to use H2S as a therapeutic agent. The ability of H2S to regulate vascular tone, inflammation, oxidative stress, and apoptosis might be particularly useful in the therapeutic management of critical illness. However, neither the inhalation of gaseous H2S, nor the administration of inorganic H2S-releasing salts or slow-releasing H2S-donors are feasible for clinical use. Na2S2O3 is a clinically approved compound with a good safety profile and is able to release H2S, in particular under hypoxic conditions. Pre-clinical studies show promise for Na2S2O3 in the acute management of critical illness. A current clinical trial is investigating the therapeutic potential for Na2S2O3 in myocardial infarct. Pre-eclampsia and COVID-19 pneumonia might be relevant targets for future clinical trials.
Nimodipine Exerts Beneficial Effects on the Rat Oligodendrocyte Cell Line OLN-93
Authors: Boltz Felix , Enders Michael , Feigenspan Andreas , Kirchner Philipp , Ekici Arif , Kuerten Stefanie
Published: April 2022

Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system (CNS). Therapy is currently limited to drugs that interfere with the immune system; treatment options that primarily mediate neuroprotection and prevent neurodegeneration are not available. Here, we studied the effects of nimodipine on the rat cell line OLN-93, which resembles young mature oligodendrocytes. Nimodipine is a dihydropyridine that blocks the voltage-gated L-type calcium channel family members Cav1.2 and Cav1.3. Our data show that the treatment of OLN-93 cells with nimodipine induced the upregulation of myelin genes, in particular of proteolipid protein 1 (Plp1), which was confirmed by a significantly greater expression of PLP1 in immunofluorescence analysis and the presence of myelin structures in the cytoplasm at the ultrastructural level. Whole-genome RNA sequencing additionally revealed the upregulation of genes that are involved in neuroprotection, remyelination, and antioxidation pathways. Interestingly, the observed effects were independent of Cav1.2 and Cav1.3 because OLN-93 cells do not express these channels, and there was no measurable response pattern in patch-clamp analysis. Taking into consideration previous studies that demonstrated a beneficial effect of nimodipine on microglia, our data support the notion that nimodipine is an interesting drug candidate for the treatment of MS and other demyelinating diseases.
Estrogens Play a Critical Role in Stress-Related Gastrointestinal Dysfunction in a Spontaneous Model of Disorders of Gut–Brain Interaction
Authors: Accarie Alison , Toth Joran , Wauters Lucas , Farré Ricard , Tack Jan , Vanuytsel Tim
Published: April 2022

Background: Disorders of the gut–brain interaction (DGBI), such as irritable bowel syndrome and functional dyspepsia, are more prevalent in women than in men, with a ratio of 2:1. Furthermore, stressful life events have been reported as one of the triggers for symptoms in DGBI patients. Methods: Here, we studied the effect of an early-life stressor (maternal separation (MS)) on jejunal and colonic alterations, including colonic sensitivity and immune cells infiltration and activation in a validated spontaneous model of DGBI (BBDP-N), and investigated the involvement of β-estradiol on stress-worsened intestinal alterations. Results: We found that maternal separation exacerbated colonic sensitivity and mast cell and eosinophil infiltration and activation in females only. Ovariectomy partially rescued the stress phenotype by decreasing colonic sensitivity, which was restored by β-estradiol injections and did not impact immune cells infiltration and activation. Stressed males exposed to β-estradiol demonstrated similar intestinal alterations as MS females. Conclusion: Estrogen plays a direct critical role in colonic hypersensitivity in a spontaneous animal model of DGBI, while for immune activation, estrogen seems to be involved in the first step of their recruitment and activation. Our data point towards a complex interaction between stress and β-estradiol in DGBI.
Transcriptomic Profiling of DNA Damage Response in Patient-Derived Glioblastoma Cells before and after Radiation and Temozolomide Treatment
Authors: Lozinski Mathew , Bowden Nikola A. , Graves Moira C. , Fay Michael , Day Bryan W. , Stringer Brett W. , Tooney Paul A.
Published: April 2022

Glioblastoma is a highly aggressive, invasive and treatment-resistant tumour. The DNA damage response (DDR) provides tumour cells with enhanced ability to activate cell cycle arrest and repair treatment-induced DNA damage. We studied the expression of DDR, its relationship with standard treatment response and patient survival, and its activation after treatment. The transcriptomic profile of DDR pathways was characterised within a cohort of isocitrate dehydrogenase (IDH) wild-type glioblastoma from The Cancer Genome Atlas (TCGA) and 12 patient-derived glioblastoma cell lines. The relationship between DDR expression and patient survival and cell line response to temozolomide (TMZ) or radiation therapy (RT) was assessed. Finally, the expression of 84 DDR genes was examined in glioblastoma cells treated with TMZ and/or RT. Although distinct DDR cluster groups were apparent in the TCGA cohort and cell lines, no significant differences in OS and treatment response were observed. At the gene level, the high expression of ATP23, RAD51C and RPA3 independently associated with poor prognosis in glioblastoma patients. Finally, we observed a substantial upregulation of DDR genes after treatment with TMZ and/or RT, particularly in RT-treated glioblastoma cells, peaking within 24 h after treatment. Our results confirm the potential influence of DDR genes in patient outcome. The observation of DDR genes in response to TMZ and RT gives insight into the global response of DDR pathways after adjuvant treatment in glioblastoma, which may have utility in determining DDR targets for inhibition.
Unlaid Eggs: Ovarian Damage after Low-Dose Radiation
Authors: Reiser Elisabeth , Bazzano Maria Victoria , Solano Maria Emilia , Haybaeck Johannes , Schatz Christoph , Mangesius Julian , Ganswindt Ute , Toth Bettina
Published: April 2022

The total body irradiation of lymphomas and co-irradiation in the treatment of adjacent solid tumors can lead to a reduced ovarian function, premature ovarian insufficiency, and menopause. A small number of studies has assessed the radiation-induced damage of primordial follicles in animal models and humans. Studies are emerging that evaluate radiation-induced damage to the surrounding ovarian tissue including stromal and immune cells. We reviewed basic laboratory work to assess the current state of knowledge and to establish an experimental setting for further studies in animals and humans. The experimental approaches were mostly performed using mouse models. Most studies relied on single doses as high as 1 Gy, which is considered to cause severe damage to the ovary. Changes in the ovarian reserve were related to the primordial follicle count, providing reproducible evidence that radiation with 1 Gy leads to a significant depletion. Radiation with 0.1 Gy mostly did not show an effect on the primordial follicles. Fewer data exist on the effects of radiation on the ovarian microenvironment including theca-interstitial, immune, endothelial, and smooth muscle cells. We concluded that a mouse model would provide the most reliable model to study the effects of low-dose radiation. Furthermore, both immunohistochemistry and fluorescence-activated cell sorting (FACS) analyses were valuable to analyze not only the germ cells but also the ovarian microenvironment.
Pulsed Sputtering Preparation of InGaN Multi-Color Cascaded LED Stacks for Large-Area Monolithic Integration of RGB LED Pixels
Authors: Morikawa Soichiro , Ueno Kohei , Kobayashi Atsushi , Fujioka Hiroshi
Published: April 2022

Micro-LEDs have been attracting attention as a potential candidate for the next generation of display technology. Here we demonstrate the feasibility of large-area monolithic integration of multi-color InGaN micro-LEDs via pulsed sputtering deposition (PSD) and a standard photolithographical technique. The PSD allows for sequential epitaxial growth of blue and green InGaN LED stacks connected with the GaN based tunneling junction. The tunneling junctions serve as protective layers on p-type GaN against the dry etching damage and hole injection layers in each blue and green emission InGaN active layer. The tunneling junction-connected multi-color InGaN LED stack contributes to the high-density and large-area monolithic integration of RGB micro-LEDs using standard photolithography and the ICP-dry etching method.
Effect of Nd and Mn Co-Doping on Dielectric, Ferroelectric and Photovoltaic Properties of BiFeO3
Authors: Wu Qiyuan , Song Yanling , Jia Caihong , Gao Zhaomeng , Zhang Weifeng
Published: April 2022

Bi1−xNdxFe0.99Mn0.01O3 (BNFMO, x = 0.00~0.20) films were epitaxially grown on Nb:SrTiO3 (001) substrates using pulsed laser deposition. It was found that the Nd-doping concentration has a great impact on the surface morphology, crystal structure, and electrical properties. BNFMO thin film with low Nd-doping concentration (≤16%) crystallizes into a rhombohedral structure, while the high Nd-doping (>16%) will lead to the formation of an orthogonal structure. Furthermore, to eliminate the resistive switching (RS) effect, a positive-up–negative-down (PUND) measurement was applied on two devices in series. The remnant polarization experiences an increase with the Nd-doping concentration increasing to 16%, then drops down with the further increased concentration of Nd. Finally, the ferroelectric photovoltaic effect is also regulated by the ferroelectric polarization, and the maximum photocurrent of 1758 μA/cm2 was obtained in Bi0.84Nd0.16Fe0.99Mn0.01O3 thin film. BNFMO films show great potential for ferroelectric and photovoltaic applications.
Low-Resolution Precoding for Multi-Antenna Downlink Channels and OFDM
Authors: Nedelcu Andrei , Steiner Fabian , Kramer Gerhard
Published: April 2022

Downlink precoding is considered for multi-path multi-input single-output channels where the base station uses orthogonal frequency-division multiplexing and low-resolution signaling. A quantized coordinate minimization (QCM) algorithm is proposed and its performance is compared to other precoding algorithms including squared infinity-norm relaxation (SQUID), multi-antenna greedy iterative quantization (MAGIQ), and maximum safety margin precoding. MAGIQ and QCM achieve the highest information rates and QCM has the lowest complexity measured in the number of multiplications. The information rates are computed for pilot-aided channel estimation and data-aided channel estimation. Bit error rates for a 5G low-density parity-check code confirm the information-theoretic calculations. Simulations with imperfect channel knowledge at the transmitter show that the performance of QCM and SQUID degrades in a similar fashion as zero-forcing precoding with high resolution quantizers.
Supporting the Development of Pre-Service Primary Teachers PCK and CK through a STEM Program
Authors: Correia Marisa , Baptista Mónica
Published: April 2022

STEM (Science-Technology-Engineering-Mathematics) education has received great attention in recent years not only for promoting interest and learning in these areas but also for encouraging children and young people to pursue careers in them. This research explored the effects of a STEM program in developing the primary pre-service teachers’ Content Knowledge (CK) and Pedagogical Content Knowledge (PCK) about sound. A qualitative and interpretative study analyzed the impact of a STEM program on the CK and PCK of 18 pre-service primary teachers that were attending a master’s degree program in a Portuguese higher education institution. The data was collected from their lesson plans, field notes, a focus group interview, and participants assignments throughout the STEM activities carried out. Findings revealed several scientific misconceptions and weaknesses in the participants’ PCK. Nevertheless, there was a clear positive impact on pre-service teachers’ CK and PCK, specifically regarding the principles underlying STEM integration that was proposed in the conceptual framework.
Connecting Higher Education to the Labour Market: The Experience of Service Learning in a Portuguese University
Authors: Pais Sofia Castanheira , Dias Teresa Silva , Benício Deyse
Published: April 2022

The mission of higher education institutions (HEI) includes fostering conditions that enable student participation, and a commitment to their professional success. Pedagogical strategies which combine learning that goes beyond the university and involves a service-learning (SL) course carried out in the context of a pedagogical innovation programme of the University of Porto were examined. This examination included interviews with teachers, a focus group discussion with students and the analysis of logbooks and final reports to realize the potential of SL for improving employability. The results put into perspective the weaknesses and potentialities of SL courses at HEI and show that when academic learning is integrated with community experience, students gain both personal/social and academic skills. They also develop leadership and communication skills and critical awareness on the one hand and time and resources management and the ability to adapt and respond to challenges of the real world on the other, all seen as beneficial for the transition into the labour market.
Analysis of LED Lamps’ Sensitivity to Surge Impulse
Authors: Sabat Wiesław , Klepacki Dariusz , Kamuda Kazimierz , Kuryło Kazimierz
Published: April 2022

The results of research on the influence of the 1.2/50 µs (8/20 µs) surge impulse on the reliability of commercial LED lamps are presented in the paper. For two types of lamps, differing in the declared warranty period, tests were carried out in which it was determined how the impulse of 1.2/50 µs (8/20 µs) is propagated in their supply systems. During the tests, the effects it causes on the critical levels and how its level influences the probability of failure of the tested lamp were examined. These solutions are currently being used in mass production of LED lamps and are characteristic of budget devices. Sensitivity tests were conducted for a group of 150 lamps divided evenly into 10 batches. The tests were carried out in accordance with the standards of EN 61000-4-5: 2014 and EN 61547: 2009 with one exception: the impulse level was not limited to the 0.5 kV recommended in EN 61547: 2009 for this class of components, but increased to a value that leads to permanent damage. These tests allowed us to determine the margin from the value specified in the standard to the sensitivity of the tested lamps on this type of disturbance.
Experimental and Numerical Analysis of Rotor–Rotor Interaction Characteristics inside a Multistage Transonic Axial Compressor
Authors: Zhao Jiayi , Lu Qingfei , Yang Dangguo
Published: April 2022

Serving as a key component of the core engine, the high-load axial compressor is expected to have high performance, which determines several critical parameter levels of the aero-engine. The unsteady effect on the performance induced by the interaction among different rotors should not be ignored during the design of a high-load compressor. The interaction between R1 (the first rotor row) and R2 (the second rotor row) rotors of a transonic axial compressor was measured in detail using high-frequency pressure fluctuation sensors, aiming to reveal the evolution and distribution characteristics of the R1 sweep effect inside the R2 passage. The results show that near choke and design points, the interaction between the R1 oblique shock wave at the leading edge and the high-pressure region on the blade pressure side triggers the R1-2BPF (blade passing frequency) disturbance, which is different from the traditional harmonic of the blade wake disturbance. A ‘long tail’ flow structure, which indicates the major influence of the R1 shock wave, fluctuation obviously reaches the exit of R1 and influences the upper part of S1 and R2. The combination of the R1-2BPF and the R1-1BPF (mainly caused by the R1 wake disturbance) influences the R2 flow field significantly, and both of them sharply grow at the middle and rear parts of the R2 passage where the strength of the two disturbances increases by 24% and 68%, respectively, compared to the leading edge of R2. Moreover, the circumferential non-uniformity of the R1-1BPF and R1-2BPF disturbances significantly increase at some locations of the R2 passage compared to the R1 exit, which is attributed to the relative clocking positions of the R1 and R2 blades.
Driving Factors of CO2 Emissions in China’s Power Industry: Relative Importance Analysis Based on Spatial Durbin Model
Authors: Chi Yuanying , Zhou Wenbing , Tang Songlin , Hu Yu
Published: April 2022

The low-carbon transformation of the power industry is of great significance to realize the carbon peak in advance. However, almost a third of China’s CO2 emissions came from the power sector in 2019. This paper aimed to identify the key drivers of CO2 emissions in China’s power industry with the consideration of spatial autocorrelation. The spatial Durbin model and relative importance analysis were combined based on Chinese provincial data from 2003 to 2019. This combination demonstrated that GDP, the power supply structure and energy intensity are the key drivers of CO2 emissions in China’s power industry. The self-supply ratio of electricity and the spatial spillover effect have a slight effect on increasing CO2 emissions. The energy demand structure and CO2 emission intensity of thermal power have a positive effect, although it is the lowest. Second, the positive impact of GDP on CO2 emissions is decreasing, but that of the power supply structure and energy intensity is increasing. Third, the energy demand of the industrial and residential sectors has a greater impact on CO2 emissions than that of construction and transportation. For achieving the CO2 emission peak in advance, governments should give priority to developing renewable power and regional electricity trade rather than upgrading thermal power generation. They should also focus on promoting energy-saving technology, especially tapping the energy-saving potential of the industry and resident sectors.
Soil Pollution Assessment and Sustainable Remediation Strategies
Authors: Alvarenga Paula
Published: April 2022

When the presence of a chemical in soil affects humans or other living organisms, producing undesired effects, that soil is considered polluted [...]
Isolation and Characterization of a Cholesterol-Lowering Bacteria from Bubalus bubalis Raw Milk
Authors: Hameed Abdul , Condò Carla , Tauseef Isfahan , Idrees Maryam , Ghazanfar Shakira , Farid Arshad , Muzammal Muhammad , Al Mohaini Mohammed , Alsalman Abdulkhaliq J. , Al Hawaj Maitham A. , Adetunji Charles Oluwaseun , Dauda Wadzani Palnam , Hameed Yasir , Alhashem Yousef N. , Alanazi Abeer A.
Published: April 2022

Probiotics retrieved from animal sources have substantial health benefits for both humans and animals. The present study was designed to identify lactic acid bacteria (LAB) isolated from domestic water buffalo milk (Bubalus bubalis) and to evaluate their potential as target-based probiotics. Forty-six LAB strains were isolated and, among them, five strains (NMCC-M2, NMCC-M4, NMCC-M5, NMCC-M6, and NMCC-M7) were regarded as possible probiotics on the basis of their phenotypic and biochemical properties. These isolates were molecularly identified as Weissella confusa (NMCC-M2), Leuconostoc pseudo-mesenteroides (NMCC-M4), Lactococcus lactis Subsp. hordniae (NMCC-M5), Enterococcus faecium NMCC-M6, and Enterococcus lactis NMCC-M7. The tested bacterial strains showed significant antimicrobial activity, susceptibility to antibiotics, acid and bile tolerance, sugar fermentation, enzymatic potential, and nonhemolytic characteristics. Interestingly, NMCC-M2 displayed the best probiotic features including survival at pH 3 and 0.5% (w/v) bile salts, complete susceptibility to the tested antibiotics, high enzymatic potential, and in vitro cholesterol reduction (48.0 µg/mL for NMCC-M2) with 0.3% bile salt supplementation. Therefore, the isolated strain NMCC-M2 could be considered as a potential target-based probiotic in cholesterol-lowering fermented food products.
Myofibrillar Protein Interacting with Trehalose Elevated the Quality of Frozen Meat
Authors: Xu Shijie , Li Ping , Han Fei , Zhou Hui , Zhou Kai , Wang Ying , Cai Kezhou , Li Cong , Xu Baocai
Published: April 2022

This work studied the interactions between trehalose/chitooligosaccharide (COS) and myofibrillar protein (MP), and the effect of such interactions on the quality of meat after freezing was also evaluated. Fourier transform infrared spectroscopy showed that both trehalose and COS could enhance the content of hydrogen bonds of MP. Zeta potential measurement displayed trehalose/COS reduced the absolute value of the surface potential of MP. The results of Raman spectroscopy suggested that the hydrophobic residues of MP were more exposed after treatment with trehalose/COS. Thus, trehalose and COS could both interact with MP through non-covalent bonds. Subsequently, the evaluation of the effect of trehalose and COS on the physicochemical properties of frozen meat was conducted. Results showed that both trehalose and COS significantly reduced thawing loss of frozen meat, and sensory evaluation showed that trehalose had a better performance from the perspective of smell, texture, and overall consumer acceptance. In conclusion, trehalose/COS interacting with MP can reduce meat thawing loss, which might provide technical guidance in the quality control of frozen meat.
Effects of Ultrasound Combined with Preheating Treatment to Improve the Thermal Stability of Coconut Milk by Modifying the Physicochemical Properties of Coconut Protein
Authors: Sun Yizhou , Chen Haiming , Chen Wenxue , Zhong Qiuping , Zhang Ming , Shen Yan
Published: April 2022

In the food industry, coconut milk has a unique flavor and rich nutritional value. However, the poor emulsifying properties of coconut proteins restrict its development. In this study, the effect of ultrasound combined with preheating on coconut globulin and coconut milk was evaluated by physicochemical properties and structural characteristics. The results showed that ultrasound and 90 °C preheating gave coconut protein better emulsifying and thermal properties, demonstrated by higher solubility (45.2% to 53.5%), fewer free sulfhydryl groups (33.24 to 28.05 μmol/g) and higher surface hydrophobicity (7658.6 to 10,815.1). Additionally, Fourier transform infrared spectroscopy and scanning electron microscopy showed obvious changes in the secondary structure. Furthermore, the change in the physicochemical properties of the protein brought a higher zeta potential (−11 to −23 mV), decreased the thermal aggregation rate (148.5% to 13.4%) and increased the viscosity (126.9 to 1103.0 m·Pa·s) of the coconut milk, which indicates that ultrasound combined with preheating treatment provided coconut milk with better thermal stability. In conclusion, ultrasound combined with preheating will have a better influence on modifying coconut globulin and increasing the thermal stability of coconut milk. This study provides evidence that ultrasound and other modification technologies can be combined to solve the problems encountered in the processing of coconut protein products.
Nutraceuticals and Diet Supplements in Crohn’s Disease: A General Overview of the Most Promising Approaches in the Clinic
Authors: De Conno Barbara , Pesce Marcella , Chiurazzi Martina , Andreozzi Marta , Rurgo Sara , Corpetti Chiara , Seguella Luisa , Del Re Alessandro , Palenca Irene , Esposito Giuseppe , Sarnelli Giovanni
Published: April 2022

Crohn’s disease (CD) is a chronic inflammatory gastrointestinal disorder requiring lifelong medications. The currently approved drugs for CD are associated with relevant side effects and several studies suggest an increased use of nutraceuticals among CD patients, seeking for what is perceived as a more “natural” approach in controlling this highly morbid condition. Nutraceuticals are foods or foods’ components with beneficial health properties that could aid in CD treatment for their anti-inflammatory, analgesic and immunoregulatory activities that come along with safety, high tolerability, easy availability and affordability. Depending on their biological effect, nutraceuticals’ support could be employed in different subsets of CD patients, both those with active disease, as adjunctive immunomodulatory therapies, and/or in quiescent disease to provide symptomatic relief in patients with residual functional symptoms. Despite the increasing interest of the general public, both limited research and lack of education from healthcare professionals regarding their real clinical effectiveness account for the increasing number of patients turning to unconventional sources. Professionals should recognize their widespread use and the evidence base for or against their efficacy to properly counsel IBD patients. Overall, nutraceuticals appear to be safe complements to conventional therapies; nonetheless, little quality evidence supports a positive impact on underlying inflammatory activity.
Suitability of Screened Monitoring Wells for Temperature Measurements Regarding Large-Scale Geothermal Collector Systems
Authors: Bertermann David , Rammler Mario
Published: April 2022

Groundwater temperature (GWT) is usually measured using screened monitoring wells (MWs). The aim of this study was to investigate whether MWs are suitable for monitoring the effects of large-scale geothermal collector systems (LSCs) on GWT, focusing on possible vertical flows within the MWs due to both natural and forced convection. Comparative temperature depth profiles were therefore recorded over a period of nine months in both shallow MWs and in small-diameter, non-screened temperature monitoring stations (TMSs), each of which was installed in a single borehole. Particularly high temperature deviations were measured in MWs in the upper part of the water column where the GWT reached up to 1.8 K warmer than in the surrounding subsurface. These deviations correlate unambiguously with the prevailing positive thermal gradients and are caused by thermal convection. Where forced convection occurred, the GWT was measured to be up to 0.8 K colder. Potential temperature deviations must be considered when monitoring very shallow GWT as thermal gradients can be particularly high in these zones. For monitoring concepts of LSCs, a combination of MW and TMS is proposed for GWT measurements decoupled by the effects of convection and in order to enable further investigations such as pumping tests.
Yield and Compositional Profile of Eggplant Fruits as Affected by Phosphorus Supply, Genotype and Grafting
Authors: Mauro Rosario Paolo , Stazi Silvia Rita , Distefano Miriam , Giuffrida Francesco , Marabottini Rosita , Sabatino Leo , Allevato Enrica , Cannata Claudio , Basile Federico , Leonardi Cherubino
Published: April 2022

The present experiment addressed the effects of two phosphorus regimes (30 and 90 kg ha−1, hereafter P30 and P90) on yield and composition of eggplant fruits in ‘Birgah’ and ‘Dalia’, whether or not these cultivars were grafted onto Solanum torvum ‘Espina’. The P30 regime did not reduce yield, and promoted fruits’ dry matter and total phenols content, along with their concentrations of macronutrients, mesonutrients (S and Na) and micronutrients (mostly Cu, B, Zn); however, their Fe concentrations were depressed. The rootstock ‘Espina’ increased fruit yield, dry matter content, epicarp chroma (in ‘Birgah’) and Ca content, together with their concentrations of B and Zn (especially at P30), but reduced their Fe content, mostly under P30. Thus, the reduced P supply and grafting proved to be effective tools to enhance fruit yield, carpometric and almost all nutritional traits in eggplant, in a framework of more sustainable crop management. However, the reduced fruit concentration of Fe suggests that the affinity of the rootstock with specific micro minerals should be taken into account, along with the option to adopt complementary practices (e.g., targeted micronutrient fertilizations) to manage the micro mineral composition of eggplants.
Alcohol Consumption on the Heaviest Drinking Occasion and Hangovers during the First Dutch COVID-19 Lockdown
Authors: Merlo Agnese , Severeijns Noortje R , Hendriksen Pauline A , Benson Sarah , Scholey Andrew , Garssen Johan , Bruce Gillian , Verster Joris C
Published: April 2022

The purpose of this study was to compare alcohol consumption between the heaviest drinking occasion in the period before the coronavirus disease 2019 (COVID-19) lockdown (15 January–14 March 2020) and the first COVID-19 lockdown period (15 March–11 May 2020) in the Netherlands, including the presence and severity of associated hangovers. The analysis included a sub-sample from the “Corona Lockdown: how fit are you?” (CLOFIT) study, comprising N = 761 participants who reported consuming alcohol in 2020. Overall, on the heaviest drinking occasion during the first COVID-19 lockdown period a significant reduction in number of alcoholic drinks consumed on the heaviest drinking occasion, drinking duration, and estimated BAC was observed. A significant reduction was also observed for subjective intoxication and next-day hangover severity. During the lockdown period, a significant reduction in the frequency of alcohol hangovers was reported. Several age and sex differences were observed. Specifically, men consumed significantly more alcohol than women and experienced hangovers significantly more frequently, both before and during the lockdown. With regard to age, young adults (18–35 years old) significantly reduced their alcohol intake on the heaviest drinking occasion during the lockdown and also reported lower ratings of subjective intoxication and hangover severity. No significant changes were seen for individuals above 35 years old. In conclusion, the first COVID-19 lockdown in the Netherlands was associated with reduced alcohol intake on the heaviest drinking occasion and a reduction in the severity of hangovers, particularly among young male adults.
Epidemiological Study of Foot Injuries in the Practice of Sport Climbing
Authors: Cobos-Moreno Paula , Astasio-Picado Álvaro , Gómez-Martín Beatriz
Published: April 2022

Background. Climbing is a multidisciplinary sport, where the main objective is to reach the highest point of a rock wall or to reach the end of an established route. There are different types of modalities: sport climbing and traditional climbing. The risks and precautions taken with respect to this sport will directly affect the epidemiology of injuries related to its practice. The present study was designed to identify and characterize the most frequent injuries in the feet of climbers and to determine if there is a relationship between the injuries that appear and the time spent practicing the sport. Methods. A total of 53 people were collected, 32 men and 21 women, corresponding to the climbers of the FEXME (Extremadura Federation of Mountain and Climbing). To determine the diagnoses, exploratory tests, classified according to the variables to be studied, are carried out: inspection variables and questionnaire variables. Results. The average number of years of climbing was seven years, and the average number of hours of training per week was 6.6 h. Some type of alterations were presented in 70% of the respondents, and foot pain was present during climbing in 45% of the participants. The p-value showed a relationship between years of climbing and the occurrence of chronic foot injuries (p = 0.035), however, there is no relationship between the occurrence of injuries and chronological age. Conclusion. We can see that the most frequent injuries in the practice of climbing are claw toes, dermal alterations such as bursitis of the first toe and hallux limitus, followed by hallux valgus. Similarly, only a significant relationship was found between the number of years of climbing and the appearance of foot injuries.