bluuebunny/crossref_metadata_2025 huggingface dataset:
High-fat diet mouse model receiving L-glucose supplementations propagates liver injury
Authors: Amer Johnny , Amleh Athar , Salhab Ahmad , Kolodny Yuval , Yochelis Shira , Saffouri Baker , Paltiel Yossi , Safadi Rifaat
Published: December 2024

Background and aims

Limited data link manufactured sweeteners impact on metabolic dysfunction-associated steatotic liver disease (MASLD). We aimed to evaluate the effects of manufactured sugars (L-glucose) compared to natural sugars (D-glucose) on phenotype, molecular and metabolic changes in mice models fed with either regular diet (RD) or high fat diet (HFD).

Methods

C57BL/6 mice fed 16-weeks with either RD; 70% carbohydrate or HFD; 60% fat, with or without additional glucose (Glu, at 18% w/v) to drinking tap water at weeks 8–16; of either natural (D-Glu) or manufactured (L-Glu) sugars. Liver inflammation (ALT and AST serum levels, liver H&E histologic stains and cell viability profile by p-AKT), liver fibrosis [quantitated α smooth-muscle-actin (αSMA) by western blot and RT-PCR, Masson Trichrome staining (MTC) of liver tissue], liver lipid [steatosis stain by H&E, Adipose Differentiation-Related Protein (ADRP) lipid accumulation, serum and lipid peroxidation Malondialdehyde (MDA) markers by ELISA], glucose hemostasis (serum Glucose and C-peptide with HOMA-IR score calculation) and liver aspects [hepatic glucose transporter 2 (GLUT2), insulin receptor (IR) expressions and GYS2/PYGL ratio] evaluated.

Results

D- and L-Glu supplementations propagate hepatocytes ballooning and steatosis in HFD-fed mice and were associated with αSMA down-expressions by 1.5-fold compared to the untreated group while showed an acceleration in liver fibrosis in the RD-fed mice. Lipid profile (Steatosis, ADRP and MDA) significantly increased in HFD-fed mice, both Glu supplementations (mainly the L-Glu) increased serum MDA while decreased ADRP. HOMA-IR score and IR significantly increased in HFD-fed mice, with further elevation in HOMA-IR score following Glu supplementations (mainly L-Glu). The increase in HOMA-IR negatively correlated with IR and Glut2 expressions. D- and L-Glu supplementations showed significant decrease of Glycogenesis (low GYS2/PYGL ratio) and unchanged p-AKT pattern compared to their RD counterparts.

Conclusion

Our data indicate an increase in rate of de-novo lipogenesis (DNL) in RD-fed mice (High carbohydrate diet) and liver fibrosis following additional sugar supplementations. In contrast, HFD-fed mice (with pre-existing high lipid profile) supplemented with sugar showed less liver fibrosis, because of reduced de-novo fatty acids synthesis and subsequently, the lipid oxidation pathways become dominated and induce the net results of lipid clearance.
The potential role of HPV oncoproteins in the PD-L1/PD-1 pathway in cervical cancer: new perspectives on cervical cancer immunotherapy
Authors: Li Jin , Ma Yuhong , Wu Qixuemeng , Ping Pengbin , Li Juan , Xu Xiaoying
Published: December 2024

Cervical cancer (CC) is a common malignant tumour of the female reproductive system that is highly harmful to women’s health. The efficacy of traditional surgery, radiotherapy and chemotherapy is limited, especially for recurrent and metastatic CC. With continuous progress in diagnostic and treatment technology, immunotherapy has become a new approach for treating CC and has become a new therapy for recurrent and metastatic CC. However, immunotherapy is not effective for all patients with CC. Therefore, factors related to immunotherapy efficacy in CC patients have become the focus of researchers. High-risk human papillomavirus (HPV) infection is an important factor that drives CC development and affects its progression and prognosis. Increasing attention has been given to the mechanism of the E5, E6 and E7 proteins, which are encoded by the HPV gene, in the occurrence and development of CC and their interaction with programmed cell death ligand-1/programmed cell death-1 (PD-L1/PD-1). Although some preliminary studies have been conducted on these topics, a comprehensive and systematic review of these topics is not available. This review comprehensively summarizes related articles from journals with impact factors greater than 3 and published in the past 5 years; it also reviews studies on the mechanism of HPV and CC, the mechanism of PD-L1/PD-1 axis regulation in CC, and the mechanism by which the interaction between HPV-related oncoproteins and the PD-L1/PD-1 pathway affects the development and prognosis of CC. This study provides theoretical support for the use of immunotherapies for CC, provides a basis for the selection of specific medications that target different HPV-related proteins, and provides a new perspective for the discovery of new immunotherapy targets for CC.
Prediction model construction for the occurrence of LARS after neoadjuvant therapy combined with laparoscopic total mesorectal excision in male patients with mid-low rectal cancer
Authors: Wang Deng-Chao , Peng Xue-Feng , Yu Miao
Published: December 2024

Background

Neoadjuvant chemoradiotherapy for rectal cancer improves surgical outcomes and reduces recurrence but can cause low anterior resection syndrome (LARS), affecting quality of life. This study aims to predict the risk of LARS in male patients with mid-low rectal cancer after laparoscopic total mesorectal excision (TME).

Methods

Clinical data from 203 male patients with mid-low rectal cancer who underwent neoadjuvant therapy and laparoscopic resection were collected. Patients were divided into training (n=143) and validation (n=60) cohorts. LARS risk factors were identified using logistic regression, and a predictive model was constructed and validated using ROC curve, Hosmer-Lemeshow test, calibration curve, and decision curve analysis (DCA).

Results

LARS occurred in 53.6% of the patients in this study. Multivariate logistic regression analysis revealed that BMI ≥ 25 kg/m², tumor distance from the anal margin < 5 cm, radiotherapy, and anastomotic leakage were independent risk factors for postoperative LARS in patients (P < 0.05). The areas under the ROC curves for the training cohort and validation cohort were 0.866 (95% CI: 0.807-0.925) and 0.724 (95% CI: 0.595-0.853), respectively, with both groups showing good goodness-of-fit test results (P > 0.05). The DCA curve indicated that the model had a high clinical utility.

Conclusions

BMI ≥ 25 kg/m², tumor distance from the anal margin < 5 cm, radiotherapy, and anastomotic leakage are independent risk factors for the occurrence of LARS after neoadjuvant therapy combined with laparoscopic TME in male patients with mid-low rectal cancer. These factors should be emphasized in clinical practice, and corresponding preventive measures should be promptly implemented.
ILF2: a multifaceted regulator in malignant tumors and its prospects as a biomarker and therapeutic target
Authors: Sun Tonglin , Li Xi , Zhang Yi , Zou Bingwen , Zhang Yan
Published: December 2024

Interleukin enhancer binding factor 2 (ILF2), formerly called nuclear factor 45 (NF45), is widely expressed in normal human tissues. ILF2 often binds to interleukin enhancer binding factor 3 (ILF3) and regulates gene expression in several ways, participating in multiple DNA and RNA metabolism pathways. Recent studies have shown that ILF2 expression is significantly upregulated in esophageal cancer, lung cancer, gastric cancer, and other malignant tumors, which can promote tumor development and tumor cell proliferation, affect the cell cycle, and induce epithelial-mesenchymal transition. ILF2 expression is closely related to tumor cell migration and invasion, neo-angiogenesis, and patient prognosis. ILF2 is expected to become a biomarker for the early diagnosis of patients with tumors and assessing their prognosis. This article reviews the role of ILF2 in malignant tumors and its related mechanisms.
Structured training on gross motor skills and physical fitness in 4–5-year-old children
Authors: Quan Sheng , Liao Yutong , Ji Yulin , Zheng Shuwen
Published: December 2024

Objective

Preschool children are in a period of rapid physical development, and improving their gross motor skills and physical fitness is quite important for their health. This study aimed to investigate the effectiveness of a structured physical training program in improving Chinese preschool children's gross motor development and physical fitness.

Method

A sample of 80 children aged 4 to 5 from Fujian, China, were randomly assigned to the intervention group (N = 41), which received a 15-week structured physical training, while the control group (N = 39) continued with their daily physical activity. The Test of Gross Motor Development-3, and the National Physical Fitness Measurement Standards Manual -Preschool Children Version (2003) were assessed before and after the intervention.

Results

A series of ANCOVA analyses revealed significant group differences in aspects of gross motor skills (F = 10.17, p < 0.01) including locomotor skills (F = 5.31, p < 0.05) and ball skills (F = 15.09, p < 0.001) after controlling the effect of the age, sex, and pre-test scores. Moreover, the results also indicated a higher improvement in young children's physical fitness (F = 91.33, p < 0.001) including their body shape (F = 5.05, p < 0.05), health-related fitness (F = 43.09, p < 0.001), and skill-related fitness (F = 61.47, p < 0.001) in the intervention group over the control group. The results demonstrated that the effect size of the structured training on young children's health-related fitness (η2 = 0.38) and skill-related fitness (η2 = 0.50) was much stronger than on children's body shape (η2 = 0.07).

Conclusion

The structured training program effectively improved young children's gross motor skills and physical fitness.
The combination of decitabine with multi-omics confirms the regulatory pattern of the correlation between DNA methylation of the CACNA1C gene and atrial fibrillation
Authors: Yang Yuling , Li Qijun , Liu Xiaoning , Shao Caixia , Yang Heng , Niu Siquan , Peng Hong , Meng Xiangguang
Published: December 2024

Background

Studies have shown that DNA methylation of the CACNA1C gene is involved in the pathogenesis of various diseases and the mechanism of drug action. However, its relationship with atrial fibrillation (AF) remains largely unexplored.

Objective

To investigate the association between DNA methylation of the CACNA1C gene and AF by combining decitabine (5-Aza-2′-deoxycytidine, AZA) treatment with multi-omics analysis.

Methods

HepG2 cells were treated with AZA to observe the expression of the CACNA1C gene, which was further validated using gene expression microarrays. Pyrosequencing was employed to validate differentially methylated sites of the CACNA1C gene observed in DNA methylation microarrays. A custom DNA methylation dataset based on the MSigDB database was combined with ChIP-sequencing and RNA-sequencing data to explore the regulatory patterns of DNA methylation of the CACNA1C gene.

Results

Treatment of HepG2 cells with three different concentrations of AZA (2.5 µM, 5.0 µM, and 10.0 µM) resulted in 1.6, 2.5, and 2.9-fold increases in the mRNA expression of the CACNA1C gene, respectively, compared to the DMSO group, with statistical significance at the highest concentration group (p < 0.05). Similarly, AZA treatment of T47D cells showed upregulated mRNA expression of the CACNA1C gene in the gene expression microarray results (adj P < 0.05). DNA methylation microarray analysis revealed that methylation of a CpG site in intron 30 of the CACNA1C gene may be associated with AF (adj P < 0.05). Pyrosequencing of this site and its adjacent two CpG sites demonstrated significant differences in DNA methylation levels between AF and sinus rhythm groups (p < 0.05). Subsequent multivariate logistic regression models confirmed that the DNA methylation degree of these three sites and their average was associated with AF (p < 0.05). Additionally, the UCSC browser combined with ChIP-sequencing revealed that the aforementioned region was enriched in enhancer markers H3K27ac and H3K4me1. Differential expression and pathway analysis of RNA-sequencing data ultimately identified ATF7IP and KAT2B genes as potential regulators of the CACNA1C gene.

Conclusion

The DNA methylation levels at three CpG sites in intron 30 of the CACNA1C gene are associated with AF status, and potentially regulated by ATF7IP and KAT2B.
Subcellular spatial regulation of immunity-induced phosphorylation of RIN4 links PAMP-triggered immunity to Exo70B1
Authors: Zhao Yi , Day Brad
Published: December 2024

RIN4 is a crucial regulator of plant immunity, playing a role in both PAMP-triggered immunity (PTI) and effector-triggered immunity (ETI). While the impact of post-translational modifications (PTMs) on RIN4 has been extensively studied, their specific effects on plant immune response regulation and the underlying mechanisms have remained unclear. In this study, we investigated the phosphorylation of RIN4 at threonine-166 (RIN4T166) in Arabidopsis transgenic lines expressing various RIN4 variants. Our pathological and molecular genetic analyses reveal that RIN4T166 phosphorylation disrupts its localization to the plasma membrane (PM) and represses plant defense activation. We found that RIN4’s PM tethering relies on Exo70B1-mediated exocytosis and the integrity of the host cytoskeletal actin network. Phosphorylation at RIN4T166 disrupts its PM localization due to reduced binding affinity with Exo70B1. This disruption was further evidenced by the 35S::RIN4T166D/rin124 transgenic line, which exhibited suppressed PTI responses similar to the exo70b1 mutant. Our findings demonstrate that RIN4’s subcellular localization is regulated by phosphorylation, suggesting that plants use a sophisticated network of signaling processes to precisely control the timing and localization of immune signaling activation. This study uncovers a mechanism by which PTI is repressed through RIN4 phosphorylation, providing new insights into the spatial regulation of RIN4 within plant immune signaling pathways.
Investigating proactive aggression in patients with borderline personality disorder and major depressive disorder using a modified version of the Taylor aggression paradigm
Authors: Boccadoro Sara , Hüpen Philippa , Raine Adrian , Habel Ute , Wagels Lisa
Published: December 2024

Introduction

Inappropriate reactive (provoked) aggression is common in various psychiatric disorders, including Borderline Personality Disorder (BPD) and, to a lesser extent, Major Depressive Disorder (MDD). Less is known about proactive (unprovoked) aggression in these patients, with mixed findings in the literature. Drawing from the current evidence, we expect higher trait aggression in both patient groups and higher behavioral proactive aggression and physiological arousal in patients with BPD compared to both MDD and healthy participants (HC).

Methods

We investigated behavioral and psychophysiological correlates of proactive aggression in 23 patients with MDD, 20 with BPD, and 21 HC using a proactive version of the Taylor Aggression Paradigm (pTAP). The pTAP consists of reaction time games in which only the participant can interfere with the ostensible opponent’s performance by modifying the blurriness of the opponent’s screen. The levels of blurriness chosen by participants reflect their proactive aggression. We collected self-report measures of aggression and other personality traits. We further adopted a transdiagnostic approach by clustering participants based on proactive aggression characteristics.

Results

Both patient groups reported higher trait aggression than HC but not higher aggression in the task nor differences in the associated physiological arousal. Trial-by-trial mixed model analyses revealed that the group characterized by higher proactive aggression traits behaved more aggressively after losing, suggesting a role of frustration or sensitivity to loss.

Discussion

Our study confirms that patients with MDD and BPD report higher aggression than HC despite the absence of observable behavioral and psychophysiological differences and highlights the ubiquity of proactive aggression characteristics across diagnoses.
Explicit metrics for implicit emotions: investigating physiological and gaze indices of learner emotions
Authors: Lal Sharanya , Eysink Tessa H. S. , Gijlers Hannie A. , Veldkamp Bernard P. , Steinrücke Johannes , Verwey Willem B.
Published: December 2024

Learning experiences are intertwined with emotions, which in turn have a significant effect on learning outcomes. Therefore, digital learning environments can benefit from taking the emotional state of the learner into account. To do so, the first step is real-time emotion detection which is made possible by sensors that can continuously collect physiological and eye-tracking data. In this paper, we aimed to find features derived from skin conductance, skin temperature, and eye movements that could be used as indicators of learner emotions. Forty-four university students completed different math related tasks during which sensor data and self-reported data on the learner’s emotional state were collected. Results indicate that skin conductance response peak count, tonic skin conductance, fixation count, duration and dispersion, saccade count, duration and amplitude, and blink count and duration may be used to distinguish between different emotions. These features may be used to make learning environments more emotionally aware.
Association between breakfast consumption frequency and handgrip strength and standing long jump: a systematic review and meta-analysis
Authors: Ren Zhongyu , Zhang Xiaoping , Wei Yanqing , Liu Shuai , Cao Bing , Wang Hejin
Published: December 2024

Background and aims

Over the past decade, numerous studies investigating the relationship between breakfast consumption frequency and handgrip strength and standing long jump have produced conflicting evidence, leading to uncertainty regarding this association. This study aimed to provide further clarity on the relationship between breakfast consumption frequency and handgrip strength and standing long jump.

Methods

A comprehensive search of the literature up to September 15, 2023, was conducted on Web of Science, PubMed, Scopus, MEDLINE, and CNKI. Six studies related to grip strength and three studies related to standing long jump performance were included in the meta-analysis. For studies defining breakfast consumption frequency as an ordinal variable, the effect sizes of the lowest and highest frequency groups were analyzed.

Results

No significant differences in handgrip strength were found between the highest and lowest breakfast consumption groups. Furthermore, unlike in men, women with the highest frequency of breakfast consumption showed significantly higher handgrip strength levels than women with the lowest frequency of breakfast consumption. No significant positive associations were found between breakfast intake frequency and standing long jump levels.

Conclusion

These results suggest that while regular breakfast consumption may enhance handgrip strength in women, it does not influence standing long jump performance in both sexes.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/, CRD42024547903
Building happier bonds: gratitude as a mediator between dyadic coping and relationship satisfaction in romantic couples
Authors: Roth Michelle , Good Nicolas , Ledermann Thomas , Landolt Selina A. , Weitkamp Katharina , Bodenmann Guy
Published: December 2024

Introduction

Gratitude has been found to be relevant for relational well-being, and there has been ongoing interest in uncovering the mechanisms by which gratitude functions in interpersonal relationships. Only recently, gratitude has been studied within the context of dyadic coping—the interpersonal process of how partners communicate their stress, support each other during stressful times, and jointly cope with stress—in romantic couples. Drawing up on theoretical models on the functions of gratitude within close relationships and previous research, we aimed to advance this line of research and examined the potential mediating role of gratitude between dyadic coping and relationship satisfaction applying a dyadic perspective. For a more nuanced understanding of gratitude, we differentiated between felt and expressed dyadic coping-related gratitude.

Methods

We used data of 163 romantic mixed-gender couples living in Switzerland. To examine the mediation model dyadically, we applied the Actor Partner Interdependence Mediation Model (APIMeM).

Results

While in the presence of gratitude as a mediator, almost no significant direct effects from dyadic coping on relationship satisfaction were found, evidence pointed to a mediating role of gratitude within this process: Provided dyadic coping was related to higher gratitude, which was in turn related to higher own and partner relationship satisfaction. The results were similar for felt and expressed dyadic coping-related gratitude.

Discussion

The finding that gratitude plays an important mediating role within the dyadic coping process offers important future directions for research as well as preventative and clinical work with couples.
Modification of gemcitabine with oxaliplatin in China for unresectable gallbladder cancer: a cost-effectiveness analysis
Authors: Chen Zhaoyan , Tian Fangyuan
Published: December 2024

Background

The incidence of gall bladder cancer (GBC), one of the most prevalent bile duct malignancies, differs with ethnicity and geographic location. To treat unresected GBC in the Chinese setting, this study aimed to assess the financial effectiveness of a combination of modified gemcitabine and oxaliplatin.

Methods

Data from a randomized controlled study in which individuals with metastatic GBC were treated with oxaliplatin and gemcitabine demonstrated improved survival. A Markov model is built to calculate the incremental cost–benefit ratio (ICER) from the viewpoint of Chinese society on the basis of clinical symptoms and disease development. One-way certainty and probability sensitivity analyses are used to describe the uncertainty in the model.

Results

Compared with those of fluorouracil (FU) and folinic acid, the utility value of modified oxaliplatin combined with gemcitabine increased by 0.22QALY throughout the course of the 10-year simulation (FA). In a Chinese healthcare setting, the cost-effectiveness ratio (ICER) is $52765.59/QALY, with a 0% chance of cost–benefit at the WTP (willing-to-pay) level of $37697.00/QALY. The ICERs predicted by sensitivity analysis were not significantly affected by cost variations related to the management of Grade 3–4 AEs, the diagnostics used, or hospitalization expenditures.

Conclusion

In a Chinese healthcare context, modified gemcitabine coupled with oxaliplatin (mGEMOX) is not a cost-effective treatment option for unresectable GBC.
Prevalence and influencing factors of oral frailty in older adults: a systematic review and meta-analysis
Authors: Zhou Yutong , Zhou Li , Zhang Wen , Chen Yao , She Keyi , Zhang Hongtao , Gao Yue , Yin Xinhong
Published: December 2024

Objective

This study aimed to conduct a systematic review and meta-analysis, assessing the pooled prevalence and influencing factors of oral frailty in older people to assist healthcare professionals in enhancing their understanding of this condition and formulating efficient interventions.

Methods

This systematic review was performed based on the Preferred Reporting Items for Systematic Reviews and Meta-analyses Statement (PRISMA) guidelines. We searched PubMed, Web of Science, The Cochrane Library, Embase, CINAHL, ProQuest, the National Knowledge Infrastructure (CNKI), WAN FANG DATA, VIP Information, SinoMed and Scopus for literature published in English or Chinese from inception to June 19, 2024. Subsequently, we evaluated the potential for bias in the cross-sectional studies that met our criteria through the Agency for Healthcare Research and Quality (AHRQ) tool. In contrast, we utilized the robust Newcastle-Ottawa scale for cohort and case–control studies. We performed statistical analyses using STATA 17 software. Prevalence was studied using a meta-analysis of a single proportion. For influencing factors, dichotomous variables were expressed as odds ratios (ORs) and 95% confidence intervals (Cls), and continuous variables were expressed as weighted mean differences (WMDs) and 95% confidence intervals (Cls).

Results

Investigation into 17 studies encompassing 24,983 participants discovered a striking 28% overall prevalence rate for oral frailty among older individuals (95% CI 20–36%, p < 0.001). Upon assessing the literature’s quality, nine articles acquired high ratings; all others received medium ratings. These findings imply a complex interplay among biological, socio-economic, lifestyle, and disease-pharmacological factors in the manifestation of oral frailty in older adults.

Conclusion

Oral frailty is prevalent in older adults and is impacted by diverse factors. Enhanced surveillance and effective interventions for oral frailty are required in older cohorts.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42024544552, identifier CRD42024544552.
The impact of university freshmen’s mental health on academic performance: an empirical study based on M University in Fujian Province, China
Authors: Deng Xue , Li Cheng
Published: December 2024

Objective

To investigate the impact of freshmen’s mental health on their short-and long-term academic performance, as well as to provide empirical evidence for improving university students’ mental health intervention tactics and higher education quality.

Methods

A multiple regression model was used to analyze student data from 3 years of enrollment at M University in Fujian Province.

Results

Different mental health problems have a significant impact on academic performance, which varies by gender, enrollment year, and subject. Somatization, anxiety, and depression have a significant negative impact on both comprehensive and single-subject scores, while anxiety, social aggression, and other variables can increase academic performance in certain settings. Some effects are notably moderated by gender and enrollment year.

Conclusion

University officials should strengthen mental health surveillance and intervention during the first few years of student enrollment to mitigate the harmful impact of mental health issues on academic performance. The moderate to strong effect sizes for variables like somatization, depression, and anxiety indicate that early interventions could be crucial in reducing their negative impact on both short-and long-term academic outcomes. Furthermore, the study discovered disparities in mental health and academic performance across students of different genders and enrollment years, emphasizing that educational personnel should design more tailored mental health support methods that consider these differences.
Examining the role of intrinsic and extrinsic cues from service requirement narratives in web-based time banking participation decisions
Authors: Jiao Huifang , Lin Meiyan , Ma Lijun , He Mei , Yu Shiguan
Published: December 2024

Introduction

Time banking, known as “Community/Neighborhood Pension,” instantiates a form of co-creation that can provide a new solution to fulfil the unmet social service needs of community members with idle resources, which is a feasible solution to alleviate pension pressure. The sustainable operation of time banks relies on the co-creation and active participation of community members. Therefore, in this study, we investigate the motivation of members to participate in web-based time banks from a service requirement narrative perspective.

Methods

We collected data of 21969 service requirement projects from publicly available information on the website of Nansha Timebank (nstimebank.com, a web-based time bank platform in China). Using the data, we built a model to assess how the intrinsic and extrinsic cues underlying service requirement narratives affect the time bank participation decisions of service providers drawing on grounded theory. Then we conducted a regression analysis to test our hypotheses.

Results

We find that participants respond positively to time coins return and narratives highlighting social connection and value fulfilment but respond negatively to service hour costs and empathy-altruism cues.

Discussion

Our findings suggest that people who receive services in web-based time banking platform should utilize different linguistic cues in service requirement descriptions to improve service exchange results.
Commercialisation fears and preferred forms of governance: a mixed methods investigation to identify a trusted Australian genomics repository
Authors: Elphinstone Brad , Walshe Jarrod , Nicol Dianne , Taylor Mark
Published: December 2024

This study aimed to identify operating conditions and governance mechanisms that would help to facilitate trust in, and willingness to donate to, a hypothetical Australian national genomic repository for health research where commercial use of data is permitted. Semi-structured telephone interviews with members of the Australian public (N = 39) clarified perceived risks and preferred repository conditions. These insights were subsequently tested experimentally in a national sample (N = 1,117). Contrary to what was expected based on the interviews, when certain baseline operating conditions were included (e.g., public management, data access committee to ensure data is restricted to human health research), none of the additional tested governance mechanisms (e.g., financial penalties for misuse) increased trust or donation willingness. Thus, providing suitable baseline conditions are in place, a feasible Australian genomic repository may not require external oversight or new legislation to optimize recruitment, even if commercial users are anticipated.
Guardians of consent: enhancing rape prevention through social control mechanisms
Authors: Bhattacharya Sudip , Singh Amarjeet
Published: December 2024

Introduction

Rape is a severe violation involving non-consensual sexual acts, often accompanied by force, threats, or coercion, leading to profound physical, emotional, and social consequences for survivors.

Aim

This review aims to examine and synthesize research on effective strategies for rape prevention and intervention, with a particular focus on social control mechanisms, legal frameworks, cultural change, educational programs, public awareness campaigns, community vigilance, victim support services, and the influence of digital media.

Methodology

A comprehensive review was conducted by searching PubMed, Scopus, PsycINFO, and Google Scholar for peer-reviewed articles, policy papers, and reports from the past 20 years. Keywords like “rape prevention,” “legal frameworks,” and “consent education” were used with Boolean operators to refine the focus. Articles addressing prevention strategies, social control, legal reforms, or public awareness with empirical findings or theoretical insights were included, while unrelated studies were excluded. From 6500 records, 39 studies were ultimately included after screening and eligibility assessments.

Results

The global and national burden of rape is substantial, with significant prevalence differences influenced by cultural, socio-economic, and legal factors. Effective prevention of rape necessitates a multifaceted approach that includes stringent legal frameworks, comprehensive education on consent, public awareness campaigns, social control, and community vigilance.

Discussion

Social control plays a critical role in these prevention strategies, encompassing both formal mechanisms like legal sanctions and informal controls such as societal norms and cultural attitudes. Challenges to these efforts include persistent rape myths, victim-blaming, inconsistent legal definitions of consent, and the evolving complexities of digital media. However, global examples such as Sweden’s consent-based legal reforms and the UK’s cultural shift through public campaigns demonstrate that these challenges can be addressed effectively. National efforts, including the “It’s On Us” campaign in the United States and legal reforms in India, further highlight the importance of tailored interventions to address specific contextual challenges.

Conclusion

Ultimately, overcoming these challenges requires an integrated strategy that combines legal reforms, educational initiatives, cultural change, and robust support systems for survivors. By learning from successful global and national models, societies can build more effective frameworks for preventing rape and ensuring justice for those affected.
The global mapping of electron precipitation and ionospheric conductance from whistler-mode chorus waves
Authors: Gillespie Dillon , Connor Hyunju Kim , Ma Qianli , Zhang Xiao-Jia , Shen Xiao-Chen , Ozturk Dogacan , Meredith Nigel P.
Published: December 2024

Auroral precipitation is the second major energy source after solar irradiation that ionizes the Earth’s upper atmosphere. Diffuse electron aurora caused by wave-particle interaction in the inner magnetosphere (L < 8) takes over 60% of total auroral energy flux, strongly contributing to the ionospheric conductance and thus to the ionosphere-thermosphere dynamics. This paper quantifies the impact of chorus waves on the diffuse aurora and the ionospheric conductance during quiet, medium, and strong geomagnetic activities, parameterized by AE <100, 100 < AE < 300, and AE > 300, respectively. Using chorus wave statistics and inner-magnetosphere plasma conditions from Timed History Events and Macroscale Interactions during Substorms (THEMIS) observations, we directly derive the energy spectrum of diffuse electron precipitation under quasi-linear theory. We then calculate the height-integrated conductance from the wave-driven aurora spectrum using the electron impact ionization model of Fang et al. (Geophys. Res. Lett., 2010, 37) and the MSIS atmosphere model. By utilizing Fang’s ionization model, the US Naval Research Laboratory Mass Spectrometer and Incoherent Scattar Radar (NRLMSISE-00) model from 2000s for the neutral atmosphere components, and the University of California, Los Angeles (UCLA) Full Diffusion Code, we improve upon the standard generalization of Maxwellian diffuse electron precipitation patterns and their resulting ionosphere conductance. Our study of global auroral precipitation and ionospheric conductance from chorus wave statistics is the first statistical model of its kind. We show that the total electron flux and conductance pattern from our results agree with those of Ovation Prime model over the pre-midnight to post-dawn sector as geomagnetic activity increases. Our study examines the relative contributions of upper band chorus (UBC) and lower band chorus wave (LBC) driven conductance in the ionosphere. We found LBC waves drove diffuse electron precipitation significantly more than UBC waves, however it is possible that THEMIS data may have underestimated the upper chorus band wave observations for magnetic latitudes below 65°.
Myo-inositol improves developmental competence and reduces oxidative stress in porcine parthenogenetic embryos
Authors: Jawad Ali , Oh Dongjin , Choi Hyerin , Kim Mirae , Ham Jaehyung , Oh Byoung Chol , Lee Joohyeong , Hyun Sang-Hwan
Published: December 2024

Objective

Myo-inositol (Myo-Ins), the most abundant form of inositol, is an antioxidant and plays a crucial role in the development and reproduction of mammals and humans. However, information elucidating the role of Myo-Ins in porcine embryonic development after parthenogenetic activation (PA) is still lacking. Therefore, we investigated the effect of Myo-Ins on porcine embryos and its underlying mechanisms.

Methods

In this study, various concentrations of Myo-Ins (0, 5, 10, and 20 mM) were added to the porcine zygotic medium (PZM3) during the in vitro culture (IVC) of porcine embryos. Several characteristics were evaluated, including cleavage rate, blastocyst formation rate, intracellular glutathione (GSH) and reactive oxygen species (ROS) levels in 4–5 cell stage embryos, total cell number, apoptotic rate in blastocysts, mitochondrial membrane potential (MMP), mitochondrial quantity, mitochondrial stress in the blastocysts, and gene expression for antioxidant and mitochondrial function markers. Additionally, the immunofluorescence of HO-1 was assessed.

Results

The results showed that Myo-Ins at concentrations of 10 and 20 mM significantly increased the blastocyst formation rate compared to the control group. Embryos supplemented with 20 mM Myo-Ins exhibited higher GSH levels and lower ROS levels than those in the control group. Myo-Ins supplementation also decreased the rate of apoptosis and the apoptotic index in the treatment groups. Additionally, embryos supplemented with 20 mM Myo-Ins showed increased mitochondrial membrane potential (MMP), greater mitochondrial quantity, and reduced oxidative stress in the mitochondria. Interestingly, the expression levels of genes related to mitochondrial function and the nuclear erythroid factor 2-related factor (NRF2) pathway were elevated in the Myo-Ins treated groups. Furthermore, immunofluorescence results indicated that 20 mM Myo-Ins significantly increased HO-1 expression in blastocysts compared to the control group.

Conclusion

In conclusion, 20 mM Myo-Ins supplementation enhanced blastocyst development and improved mitochondrial function by regulating apoptosis, reducing oxidative stress, and activating the NRF2 pathway.
Hair cortisol of pigs in mixed organic farms: the influence of season, breeding system and sex
Authors: Nadlučnik Eva , Vake Tilen , Šket Ana , Žižek Ana , Snoj Tomaž , Štukelj Marina
Published: December 2024

Introduction

Measurement of hair cortisol concentration (HCC) is a useful tool for assessing the activity of the hypothalamic-pituitary-adrenal axis and thus evaluating the long-term adrenocortical response in different animal species and breeds. Robust indigenous pig breeds are highly adapted to the local environment and are preferred for organic farming, compared to the commercial breeds. We investigated whether seasonality, breeding system (indoor or outdoor) and sex influence HCC of pigs reared on organic farms.

Materials and methods

Fifty-three pigs of the indigenous Slovenian Krškopolje breed were divided into three groups. Group Ind (n = 15) was housed indoors, groups Out-1 (n = 18) and Out-2 (n = 20) were housed outdoors on two different farms for the duration of 9 months. Hair was sampled once per season in the withers area of each pig and HCC was determined using a previously validated ELISA.

Results and discussion

The effect of seasonality was found to be significant and more pronounced in pigs reared outdoors. HCC were highest and varied most in winter for all groups, while they were lowest and varied less in summer and autumn. The highest HCC was measured in group Out-1 in winter, as it was the only group housed outdoors at that time. Group Ind had significantly higher HCC in summer compared to the groups Out-1 and Out-2, which could be due to hair cortisol degradation by the UV light exposure in outdoor groups. Sex had no effect on HCC. Our study suggests that seasonality and housing type influence the HCC of pigs.
Fair and Transparent Student Admission Prediction Using Machine Learning Models
Authors: Raftopoulos George , Davrazos Gregory , Kotsiantis Sotiris
Published: December 2024

Student admission prediction is a crucial aspect of academic planning, offering insights into enrollment trends, resource allocation, and institutional growth. However, traditional methods often lack the ability to address fairness and transparency, leading to potential biases and inequities in the decision-making process. This paper explores the development and evaluation of machine learning models designed to predict student admissions while prioritizing fairness and interpretability. We employ a diverse set of algorithms, including Logistic Regression, Decision Trees, and ensemble methods, to forecast admission outcomes based on academic, demographic, and extracurricular features. Experimental results on real-world datasets highlight the effectiveness of the proposed models in achieving competitive predictive performance while adhering to fairness metrics such as demographic parity and equalized odds. Our findings demonstrate that machine learning can not only enhance the accuracy of admission predictions but also support equitable access to education by promoting transparency and accountability in automated systems.
Zinc Deficiency in Calcareous Soils: A Bibliometric Analysis from 1989 to 2024
Authors: Martínez-Ríos Osbaldo , Bravo-Vinaja Ángel , San-Martín-Hernández Cesar , Hidalgo-Moreno Claudia Isabel , Sánchez-de-Jesús Marco Antonio , Llampallas-Díaz Joseph David , Santillan-Balderas Diana Rosa , García-Preciado José Concepción
Published: December 2024

Zinc (Zn) deficiency in crops is a global issue, particularly in plants grown in calcareous soils, where Zn is often adsorbed or precipitated by calcium carbonates. The aim of this study was to identify and quantify, through bibliometric analysis, the scientific production related to Zn deficiency in calcareous soils over the last 36 years (1989–2024). A total of 374 documents were retrieved through a search on the Web of Science (WOS) platform, specifically in the Science Citation Index Expanded (SCIE) and Social Science Citation Index (SSCI) databases. Of these, only 198 articles were directly relevant to the topic and were used for the analysis. Unidimensional and multidimensional bibliometric indicators were evaluated using Excel and VOSviewer software. The results confirm that the number of articles has increased in recent years. The most influential authors, journals, articles, institutions, and countries in this research area were identified. In addition, collaboration networks between authors and countries, as well as the predominant research topics, were determined. This study provides a comprehensive overview of this field on a global scale and serves as a useful reference for scientists interested in conducting future research on related topics.
Understanding Greenhouse Tomato (Solanum lycopersicum L.) Growers’ Perceptions for Optimal Phthorimaea absoluta (Meyrick) Management—A Survey in Greece
Authors: Simoglou Konstantinos Β. , Stavrakaki Marianna , Alipranti Konstantina , Mylona Kyriaki , Roditakis Emmanouil
Published: December 2024

The tomato (Solanum lycopersicum L.) leafminer, Phthorimaea absoluta (Meyrick) (Lepidoptera: Gelechiidae), is an extremely invasive pest and is threatening tomato production worldwide. Several strategies have been developed and implemented for managing P. absoluta. Effective pest control strategies based on integrated pest management (IPM) schemes include different cultural practices and techniques. This study seeks to (a) examine the perspectives, attitudes, and opinions of greenhouse tomato producers in Greece regarding different methods of P. absoluta management and (b) to determine whether the perceived damage caused by P. absoluta could be predicted by the implementation of various control management strategies. A questionnaire was employed to ascertain the views and beliefs of the participants. The questionnaire comprised five-point Likert scale questions. Principal component analysis (PCA) and binary logistic regression analysis were applied to examine the relationship between the reported perceived insect damage and the utilization of control techniques. The study findings indicate that greater adoption of alternative control methods and higher levels of adherence to preventive measures against P. absoluta are associated with a perception of reduced levels of damage. Conversely, individuals with a strong reliance on pesticides use are more likely to perceive a higher level of damage. Ultimately, as tomato growers are trying to deal with P. absoluta, more education and research on other alternatives could help the reduction of chemical use, promoting more environmentally friendly practices. The findings highlight integrated pest management over pesticides for sustainable tomato production. This can guide future research and extension efforts to develop tailored P. absoluta management approaches for Greek greenhouse growers and similar environments.
Influence of Effective Microbial Additives Inoculation on Indigenous Bacterial Community Dynamics and Co-Occurrence Patterns During the Composting of Mixed Food Waste and Livestock Manure
Authors: Bang Donggyu , Chung Woojin , Chang Soonwoong
Published: December 2024

Sustainable management of organic waste, such as food waste (FW) and livestock manure (LS), is essential for reducing pollution and promoting resource recycling. This study investigated the effects of Bacillus sp. inoculation and biochar addition on composting efficiency, microbial dynamics, and physicochemical properties. Bacillus sp. accelerated the breakdown of cellulose and lignin, reduced moisture content, stabilized pH, and mitigated ammonia volatilization. Biochar reduced ammonia emissions by 17.04%, increasing to 28.89% with Bacillus sp. Next-generation sequencing revealed Bacillus sp. enhanced microbial diversity, suppressed pathogens, and promoted beneficial microbial interactions. LS treatments retained Firmicutes dominance (up to 95.17%), improving nitrogen retention, while FW treatments transitioned to Proteobacteria and Bacteroidota, driving plant material decomposition. By day 35, Bacillus sp. increased late-stage microbial taxa (Deinococcota, Myxococcota), linked to cellulose degradation and pathogen suppression. In FW biochar compost (FWBC), Planococcaceae and Bacillaceae synergistically decomposed complex organic matter. LS biochar compost (LSBC) reduced anaerobic families like Clostridiaceae and Peptostreptococcaceae, lowering methane and hydrogen sulfide emissions. Microbial network analysis highlighted improved cooperation under Bacillus sp., with LSBC sustaining positive interactions at higher dosages. These results demonstrate that microbial inoculants and biochar enhance composting efficiency, nutrient cycling, and environmental sustainability.
Meta-Study on Sulphur Supply of Various Crop Species in Organic Farming Between 1998 and 2023 in European Countries—Part 1: Effects of Sulphur Supply on Plant Dry Biomass, Nitrogen Uptake, Legume N2 Fixation and Sulphur Fertiliser Requirement Determinations
Authors: Kolbe Hartmut
Published: December 2024

Sulphur is an essential nutrient that fulfils various important functions in plants, including the formation of amino acids, proteins, chlorophyll and the support of nitrogen uptake, e.g., in legumes. The sulphur content of the atmosphere due to industrial combustion has fallen sharply in recent decades, which has ultimately led to yield and quality deficiencies on farms. In this summarised study, data from 98 sites in Europe were recorded from 1998 to 2023. The sulphur fertiliser trials were conducted on farms, and experimental stations under organic farming conditions. A total of 1169 treatment variants and 598 standard variants without S-fertilisation were analysed. Fertilisation was carried out with various sources of sulphur in different quantities and forms, usually directly before or during crop cultivation. The amounts of plant-available S in the soil were determined at depths of 0–90 cm. Site characteristics such as Smin, Nmin, soil type, pH value, precipitation and the extent of livestock farming were recorded. A sufficient amount of data was available for each experimental aspect to quantitatively describe the influence of increasing S supply to the soil or plant species groups (permanent grassland, lucerne-clover-grass, grain legumes and cereals) from severe deficiency to oversupply. The analyses therefore focused on establishing relationships between yield responses, correlations with the nitrogen uptake of crop species and N2 fixation in legumes and the nutrient supply with plant-available sulphur. An assessment procedure was drawn up for soil supply with available sulphur that is too low (classes A, B), optimal (class C: 20–30 kg S ha−1) and too high (classes D, E). The results were also used to develop practical methods for determining fertiliser requirements for different crop species and the crop rotation in organic farming.