bluuebunny/crossref_metadata_2025 huggingface dataset:
Remote Sensing Image Segmentation for Geospatial Entities
Authors: Geng Yu , Lv Jingguo , Wang Chen , Zhang Chuandong
Published: January 2024

Remote sensing image segmentation supports image interpretation.However, current methods yield results limited to segmented maps, showing only objects' boundary contours and positions.In remote sensing images, there are deeper connections between objects, such as spatial distance and topological relationships. Extracting the relationship features between objects on the basis of segmentation can significantly enhance image information. Since geographical entities contain rich attribute and spatiotemporal relationship features, which can compensate for the shortcomings of current remote sensing image segmentation, this paper proposes a remote sensing image segmentation algorithm oriented towards geographical entities. Through this method, rich and dynamic segmentation results are obtained, including three main aspects: first, segmenting the boundary contours of geographical entities using an attribute attention mechanism, extracting semantic, geometric, and visual information of entities; second, establishing a temporal attribute matrix to describe changes in the image over time; third, extracting orientation distance, topological, and interaction relationships between entities based on a semantic network model. Finally, the results obtained by this method include an additional segmentation information table based on the segmented map, which can comprehensively demonstrate the interaction relationships between entities in the image, providing new insights for remote sensing image interpretation.
Endovascular recanalization in vertebral artery stump syndrome: a single-center experience
Authors: Ji Renjie , Xu Ziqi , Chen Hanfeng , Luo Benyan
Published: January 2024

Objective
To evaluate the feasibility, success rate, and safety of endovascular revascularization of patients with vertebral artery stump syndrome (VASS).
Methods
This single-center retrospective study analyzed clinical and imaging data from consecutive patients with VASS who underwent endovascular recanalization from January 2020 until June 2023.
Results
Our study enrolled 30 patients [mean age 69 (range 51–84) years; 26 men]. The rate of successful technical revascularization was 96.7% (n = 29), and the rate of complications was 3.3% (n = 1). At the 6-month follow-up, the patients with successful endovascular revascularization of VASS did not have any neurological symptoms, and computed tomography angiography showed 3/29 (10.3%) re-occlusions and 4/29 (13.8%) restenosis of the stent, which was confirmed by digital subtraction angiography.
Conclusions
Endovascular recanalization in patients with VASS is feasible and has a high procedural success rate and low rate of complications. A large, multicenter, randomized study is warranted to confirm these findings.
Anatomical variants of the medial synovial fold of the posterior cruciate ligament: Morphological Classification Based on Magnetic Resonance Imaging of the Knee
Authors: Er Ali , Bozdag Mustafa , Basa Can Doruk , Kacmaz Ismail Eralp , Zhamilov Vadym , Coskun Ozge , Ekizoglu Oguzhan
Published: January 2024

Purpose
The aim of this study was to evaluate the necessity of reporting the medial synovial fold of the posterior cruciate ligament on magnetic resonance imaging of the knee joint and the relationship of its frequency with side and sex.
Methods
All magnetic resonance imaging of the knee joint were retrospectively reviewed independently by two musculoskeletal radiologists. The medial synovial fold of the posterior cruciate ligament was divided into 3 types according to the imaginary line drawn between the lateral border of the medial femoral condyle and the medial tibial intercondylar tubercle.
Results
The distribution of the frequency of medial synovial fold in all patients according to type was type A (30.1%), type B (55.4%) and type C (14.5%). Intra- and inter-observer agreement was high. Between sex, medial synovial fold types were statistically significantly higher in men.
Conclusion
Medial synovial folds of the posterior cruciate ligament can be seen on various types of magnetic resonance imaging of the knee joint and may need to be mentioned in reports, especially in patients undergoing knee surgery for various reasons.
Benchmarking Robustness of Deep Neural Networks in Semantic Segmentation of Fluorescence Microscopy Images
Authors: Zhong Liqun , Li Lingrui , Yang Ge
Published: January 2024

Fluorescence microscopy (FM) is an important and widely adopted biological imaging technique. Segmentation is generally the first step in quantitative analysis of FM images. Although deep neural networks (DNNs) have become the state-of-the-art tools for image segmentation, their performance on natural images may collapse under certain image corruptions or adversarial attacks. This poses real risks to their deployment in real-world applications. Although the robustness of DNN models in segmenting natural images has been studied extensively, their robustness in segmenting FM images remains poorly understood. To address this deficiency, we have developed an assay that benchmarks robustness of DNN segmentation models using both realistic synthetic and real FM images with precisely controlled corruptions or adversarial attacks. Specifically, we have benchmarked robustness of ten representative models such as DeepLab and Vision Transformer in semantic segmentation of FM images. We find that models with good robustness on natural images may perform poorly on FM images. We also find new robustness properties of DNN models and new connections between their corruption robustness and adversarial robustness. Based on comprehensive benchmarking of robustness of the selected models, we make specific recommendations on selection and design of robust models for segmentation of FM images.
Determinants of neonatal Seizure among neonates admitted to neonatal intensive care units in the Awi Zone Hospitals, 2023: A multi-center unmatched case control study.
Authors: Alene Tamiru , Tsega Tilahun Degu , terefe Tamene Fetene , Dessalegn Nigatu , Alem Zemenu Addis , Tamir Workineh , Amare Abraham , Mitiku Yefiru Andualem , Aynalem Zewudu Bishaw , Melaku Workineh Necho , Amare Getachew , Alemeu Biresaw Wassihun
Published: January 2024

Background: Neonatal seizure is a common medical emergency that signals severe insult to the neonatal brain. It is a major risk factor for neonatal morbidity and mortality. It has a wide worldwide variation, ranging from 5 per 1,000 live births in the United States of America to 39.5 per 1,000 live births in Kenya. To decrease this significant figure, it is better to investigate its causes further. Therefore, this study aimed to assess its determinants since there was no prior evidence about it in the context of study area.
Objective: Aim to assess the determinants of neonatal seizures among neonates admitted to neonatal intensive care units in the Awi Zone Hospitals, 2023. Methods: An institution based unmatched case-control study was conducted on 531 admitted eligible neonates from January 1, 2023, to May 30, 2023. A pretested tool was employed to collect data. The collected data were coded, edited, and entered into Epi-data version 3.1 and then exported to SPSS 26. Chi-square and odds ratios were used to assess the relationship between factors associated with the occurrence of neonatal seizure. Model goodness of fit was tested by Hosmer and Lemeshow. Bivariate and multivariate analysis was declared at P<0.25 and P<0.05 respectively to show a significant association with neonatal seizure at a 95% level of significance.
Results: A total of 506 (130 cases and 376 controls) of admitted neonates were used in the final analysis model. Neonates admitted within 24 hours of birth [AOR; 5.98 (95%, CI: 2.18-16.43)], gestational age <32 weeks [AOR; 2.89 (95%, CI: 1.29-6.53)], body temperature >37.5℃ [AOR; 4.82 (95%, CI: 1.82-12.76)], blood glucose level < 40g/dl [AOR; 4.95 (95%, CI: 2.06,11.88)], neonatal sepsis [AOR; 2.79 (95%, CI: 1.46-5.35)] and perinatal asphyxia [AOR; 8.25 (95%, CI: 4.23, 16.12)] were found to be determinants of neonatal seizure.
Conclusion and recommendations: In this study, neonatal seizure was determined by the factors of neonatal age, gestational age<32 weeks, body temperature >37.5°C, blood glucose level < 40 g/dl, neonatal sepsis, and perinatal asphyxia. Therefore, the presence of such factors requires prompt recognition and treatment. Key words: Seizure, neonates, determinants, case control, Ethiopia.
Photophobia is associated with lower sleep quality in individuals with migraine: Results from the American Registry for Migraine Research (ARMR)
Authors: Sharp Nina , Burish Mark J , Digre Kathleen B , Ailani Jessica , Fani Mahya , Lamp Sophia , Schwedt Todd J.
Published: January 2024

Background. Patients with migraine often have poor sleep quality between and during migraine attacks. Furthermore, extensive research has identified photophobia as the most common and most bothersome symptom in individuals with migraine, second only to headache. Seeking the comfort of darkness is a common strategy for managing pain during an attack and preventing its recurrence between episodes. Given the well-established effects of daily light exposure on circadian activity rhythms and sleep quality, this study aims to investigate the relationship between photophobia symptoms and sleep quality in a cohort of patients with migraine.
Methods. A cross-sectional observational study was conducted using existing data extracted from the American Registry for Migraine Research (ARMR). Participants with a migraine diagnosis who had completed the baseline questionnaires (Photosensitivity Assessment Questionnaire (PAQ), Generalized Anxiety Disorder-7 (GAD-7), Patient Health Questionnaire-2 (PHQ-2)), and selected questions of the ARMR Sleep questionnaire were included. Models were created to describe the relationship of photophobia and photophilia with various sleep facets, including sleep quality (SQ), sleep disturbance (SDis), sleep onset latency (SOL), and sleep-related impairments (SRI). Each model was controlled for age, sex, anxiety, and depression.
Results. A total of 852 patients meeting the inclusion criteria were included in the analysis (mean age (SD) = 49.8 (13.9), 86.6% (n = 738) female). Those with photophobia exhibited significantly poorer sleep quality compared to patients without photophobia (p < 0.001). Photophobia scores were associated with SQ (p < 0.001), SDis (p < 0.001), SOL (p = 0.011), and SRI (p = 0.014), after controlling for age, sex, depression, and anxiety, signifying that higher levels of photophobia were associated with worse sleep-related outcomes. Conversely, photophilia scores were associated with better sleep-related outcomes for SQ (p < 0.001), SDis (p < 0.001), and SOL (p = 0.016).
Conclusion. Results suggest that photophobia is a risk factor for sleep disorders in those with migraine. These results underscore the necessity for comprehensive and systematic investigations into the intricate interplay between photophobia and sleep to enhance our understanding and develop tailored solutions for individuals with migraine.
Explainable Machine Learning to Uncover Distinct Phenotypic Signatures of Hypertrophic Cardiomyopathy and Fabry Disease
Authors: Ramchand Suraj , Ploszajski Matthew , Virdi Avraj , Cole Duncan , Xie Xianghua
Published: January 2024

Background: Fabry Disease (FD) is a rare metabolic condition, characterised by a wide range of symptoms and progressive deterioration of the heart, kidneys and nervous system. The condition often goes undiagnosed for between 3-10 years, with misdiagnoses during this period being common. A historically common misdiagnosis for FD is Hypertrophic Cardiomyopathy (HCM). This condition mimics the cardiac symptoms of FD, although it does not share the same genetic pathology. Machine learning (ML) is a subfield of artificial intelligence that has shown promise in identifying rare diseases, including FD. This study builds on previous research to identify FD in routine clinical test data and provide a robust explainability framework.
Methods: Cardiac test data (ECG, Holter and Echo) was extracted for 100 HCM and 49 FD patients. Stratified repeated K-fold cross-validation procedure was employed to select the optimum ML classifier. Following this, we explored a range of ML models and ensembling approaches. Each tuned classifier was trained on this data to identify differences between the experimental groups. Feature importance, SHAP values and partial dependence plots were generated to explain the model's decision-making process.
Results: Following cross-validation, the Extreme Gradient Boosting (XGB) classifier emerged as the most reliable classification algorithm (AUC and F1 score: 0.92). T-axis, gender and age were revealed as consistently being the primary features used by the model for classification predictions.
Conclusion: XGB proved to be a reliable classifier between FD and HCM for this dataset. The most important features largely aligned with established clinical approaches to differentiating phenotypes between HCM and FD. Future work should aim to compare the performance of this model against expert clinicians to compare these methods to the human state of the art.
Risk perception, well-being, depression and anxiety in children and adolescents with rheumatic diseases during the COVID-19 pandemic - results from the prospective multicenter KICK-COVID study in Germany
Authors: Sengler Claudia , Klotsche Jens , Pedersen Malthe Jessen , Niewerth Martina , Göldel Julia , Windschall Daniel , Haas Johannes-Peter , Dressler Frank , Trauzeddel Ralf , Hospach Anton , Weller-Heinemann Frank , Lanzinger Stefanie , Kamrath Clemens , Holl Reinhard W , Warschburger Petra , Minden Kirsten
Published: January 2024

Objective
To investigate the psychosocial burden in children and adolescents with juvenile rheumatic diseases during the COVID-19 pandemic.
Methods
As part of the multicentre observational KICK-COVID study linked to the National Pediatric Rheumatology Database, adolescents < 21 years and parents of children < 12 years with rheumatic diseases answered questions on perceptions of health risk (PHR) due to SARS-CoV2, stress, well-being (WHO-5) and symptoms of depression (PHQ-9) and anxiety (GAD-7). Data were collected at routine visits from June to December 2021 and assessed for association with demographic and clinical parameters, treatment and patient-reported outcomes.
Results
Data from 1356 individuals (69% female, 50% adolescents) were included. Median PHR on a numeric rating scale (NRS, 0–10) was 4 (IQR 2–6), median perceived stress was 3 (IQR 1–6). Adolescents reported a worse well-being with a significantly lower median WHO-5-score (60, IQR 40–76) than parents reported for their children < 12 years (80, IQR 68–84). Moderate to severe symptoms of depression and anxiety were reported by 14.3% and 12.3% of the adolescents, respectively. PHR was significantly higher in patients with systemic lupus erythematosus, methotrexate or biologic disease-modifying anti-rheumatic drug therapy than in patients without these characteristics, whereas lower WHO-5 or higher PHQ-9 or GAD-7 scores were only associated with poorer patient-reported health status and physical functioning.
Conclusion
The perception of health risk due to SARS-CoV2 infection was not paralleled by an impairment of mental health and well-being, which were, however, significantly correlated with self-rated health status and functional capacity, highlighting the importance of patient-reported outcome assessment.
Trial registration:
German Clinical Trials Register (DRKS), no. DRKS00027974. Registered on 27th of January 2022.
Divergent renal localization patterns of heterozygote-derived two distinct AA amyloids in a cat
Authors: Kobayashi Natsumi , Kaneda Masahiro , Iwaide Susumu , Itoh Yoshiyuki , Hisada Miki , Kato Yuka , Masoud Niki Sedghi , Tsuneyasu Machie , Akamine Tomoko , Murakami Tomoaki
Published: January 2024

Amyloid A (AA) amyloidosis poses a fatal threat to both humans and animals. While the kidneys represent the principal organ affected in AA amyloidosis, there exists variability in the localization of amyloid deposition, with distinct symptoms delineated by the specific deposition sites. Nevertheless, the factors contributing to the diversity of deposition remain unclear. In this study, we identified an association between serum amyloid A (SAA) polymorphisms and patterns of amyloid deposition. Histopathological analysis of the kidneys from a 5-year-old spayed female Japanese cat, which succumbed to systemic AA amyloidosis, revealed renal amyloid deposition in cortical glomeruli and medullary interstitium. Genetic analysis disclosed that the afflicted cat possessed a heterozygous SAA with three amino acid substitutions (K47I, Q63R, S93N), resulting in the SAAKQS and SAAIRN variants. Mass spectrometry and immunohistochemistry demonstrated that SAAKQS was deposited in the glomerulus and renal papilla, while SAAIRN was restricted to the extramedullary zone. This study established the differing renal distributions of two AA amyloid variants originating from heterozygotes within a single individual. The evidence supports the notion that the primary structure of precursor proteins defines the distribution of amyloid deposition.
Effects of NaClO 2 bleaching temperature on the properties of epoxy/lignin transparent wood composites for UV-shielding window applications
Authors: Van Hai Le , Cho Seung-Woo , Kwon Gu-Joong , Bandi Rajkumar , Kim Jeong-Ki , Han Song-Yi , Dadigala Ramakrishna , Lee Da-Young , Ma Seo-Young , Lee Seung-Hwan
Published: January 2024

The effect of NaClO2 bleaching temperature on the properties of epoxy-resin (ER) transparent wood (TW) and ER/lignin-TW for ultraviolet-shielding (UV-shielding) window applications is investigated. Higher bleaching temperatures result in greater lignin removal and TW transmittance, with a reduced TW UV-shielding functionality. To enhance the UV shielding in TW while maintaining transmittance, bleached wood is impregnated with an ER/lignin combination to produce UV-shielding TW, which was compared with three glass-window types to demonstrate UV-shielding efficiency. The results show that a 12 mm thick glass door, double-layered glass windows, and 2 mm glass windows transmit significant UV radiation (220–250 µW/cm2, 260–270 µW/cm2, and 810–820 µW/cm2, respectively). In contrast, ER-TW and ER/lignin-TW provide 80–99% and 100% UV-shielding, respectively, across all UV-radiation regions. A 2-mm-thick ER-TW and ER/lignin–TW demonstrated excellent potential for UV-shielding window applications compared to regular glass windows.
Size and build strategy effects for the L-PBF process applied to Inconel 625 vertical struts: a combined numerical and experimental approach
Authors: Peyre Patrice , Silva Julien Rodrigues Da , Hamouche Zehoua , Helbert Anne-Laure , Daligault Julien , Dal Morgan , Coste Frédéric , Baudin Thierry
Published: January 2024

A combined numerical and experimental analysis of melt-pool dimensions and resulting solidification conditions was carried out on small laser powder bed fusion (L-PBF) struts (0.2 mm to 2 mm diameters), considered as single constitutive parts of the structure lattice. In the beginning, the high-speed imaging monitoring of melt pools was performed on a dedicated instrumented L-PBF set-up for various scan strategies. In the subsequent stage, a numerical thermal model was employed on COMSOL Multiphysics® to determine the alteration of the melt pool by the struts' diameter and scanning strategy for constant (power, scan speed) conditions. A good agreement was obtained between experimental and numerical melt-pool areas. This allowed validation of calculated local cooling rates and thermal gradients near the solidification front. A clear difference was shown between outside-in or inside-out strategies, and contour-hatching in terms of local solidification conditions. Higher cooling rates were obtained for outside-in conditions, especially near the external part of struts whereas inside-out conditions promoted more uniform cooling rates and thermal gradients. Moreover, a reduction of strut diameter induced the formation of a single melt-pool on the full strut’s surface, which promoted lower and more uniform cooling rates and a highly textured built material. A fairly good agreement was found between simulated thermal data and local microstructure development at the scale of solidification cells. Finally, the current work provides a deeper understanding on size and L-PBF strategy versus microstructure formation, and allows adapting build conditions on strut diameters.
Hybrid Fuzzy testing method based on decision Tree
Authors: jingus Jaydar , Shu Fei , Zhu Yayun , Li Zhongwei
Published: January 2024

The traditional fuzzy testing has insufficient code coverage for the target program testing, low directivity to the key code and low mining efficiency. In order to solve the above problems, a method of selecting symbol execution and Fuzzing testing based on decision tree is proposed in this paper. By collecting the indexes reflecting fuzzy test and symbol execution in a fixed time window, as the input of generating decision tree, and using the classification results of decision tree, automatic scheduling of fuzzy test and symbol test in vulnerability mining is realized. That is, the path set suitable for the fuzzy test (or symbol execution) is determined before the vulnerability detection begins, so as to guide the fuzzy test (or symbol execution) to the area suitable for them. At the same time, a path simplification and deduplication algorithm is proposed to improve the efficiency of vulnerability mining. The experimental results show that the proposed method significantly improves the coverage, efficiency and vulnerability mining ability of fuzzy testing compared with single symbol execution or fuzzy testing.
Bifurcation and stability analysis of a railway wheelset with active yaw control
Authors: Guo Peng , Shi Huailong , Huang Caihong , Cao Hongjun , Zeng Jing
Published: January 2024

This paper investigates the bifurcation characteristics of a railway wheelset under active yaw control to evaluate the control laws for hunting motion. The creep forces between wheels and rail expressed as nonlinear functions of creepages are further modified. The piecewise exponent model handled by regularization method can accurately simulate the flange force. The control force provided by actuators is represented as a nonlinear function of state variables, including primary terms, quadratic terms and cubic terms. At first, the increase of linear control gains can observably increase the speeds of Hopf bifurcation and limit point of cycle bifurcation and reduce the limit cycle amplitude. The subcritical Hopf bifurcation can be transformed into supercritical by the linear control gains. The effect of displacement and velocity control gains on hunting frequency is opposite. Secondly, the limit point of cycle bifurcation speed can be remarkably affected by the quadratic nonlinear control gains. The quadratic terms can maintain the Hopf bifurcation speed constant. Only one term can achieve the transition of Hopf bifurcation form. In particular, the quadratic term disrupts the symmetry of limit cycles and induces complex nonlinear behaviors such as period-doubling bifurcation. Thirdly, increasing the cubic nonlinear control gains can slightly increase the limit point of cycle bifurcation speed and change the Hopf bifurcation type. The Hopf bifurcation speed is not affected by the cubic terms. Notably, there is a significant discrepancy in the order of magnitude required for control gains to reduce the control force and limit cycle amplitude. Finally, the combination control of linear and cubic nonlinear gains can noticeably increase the Hopf bifurcation speed while reducing the limit cycle amplitude. Hence, the reasonable combination control of linear and cubic nonlinear gains is extremely helpful to maintain the hunting stability.
Return to work and psychosocial trajectories after breast cancer: a longitudinal and sequential approach
Authors: Rubion Elise , Bourdon Marianne , Sébille Véronique , Blanchin Myriam , Bertin Mélanie
Published: January 2024

Purpose
We aimed to describe the psychosocial adjustments according to return-to-work (RTW) trajectories in breast cancer survivors (BCS) using a sequential and temporal approach.
Methods
We used BCS data included from February 2015 to April 2016 in the Longitudinal Study on Behavioural, Economic and Sociological Changes after Cancer (ELCCA) cohort. RTW trajectories were identified using the sequence analysis method followed by a clustering. Anxiety and depression were assessed using the Hospital Anxiety and Depression Scale and the EORTC quality of life questionnaire was used at inclusion and all follow-up visits to assess Health-Related Quality of Life (HRQoL).
Results
Fifty-two BCS were included in the study among whom four clusters of RTW trajectories were identified and labelled: slow RTW (N = 10), quick RTW (N = 27), partial RTW (N = 8) and part-time work (N = 7). Quick and slow RTW clusters showed slightly lower baseline mean levels of anxiety and higher levels of HRQoL. In the 4 years following diagnosis, BCS in the quick RTW cluster tended to report higher HRQoL in terms of functioning and less symptoms of pain and fatigue while those in the partial RTW cluster showed a lower HRQoL on almost all dimensions. All clusters showed an increase in pain and fatigue symptoms until 6 months followed by a tendency to recover baseline levels.
Conclusions
The results of this study suggest that BCS who return to full-time work (slow and quick RTW patterns) recover better than patients who return to part-time work (partial and part-time RTW patterns).
Transcriptome analysis reveals multiple targets of erythritol-related transcription factor EUF1 in unconventional yeast Yarrowia lipolytica
Authors: Rzechonek Dorota Alicja , Szczepańczyk Mateusz , Borodina Irina , Neuvéglise Cecile , Mirończuk Aleksandra Maria
Published: January 2024

Background
Erythritol is a four-carbon polyol with an unclear role in metabolism of some unconventional yeasts. Its production has been linked to the osmotic stress response, but the mechanism of stress protection remains unclear. Additionally, erythritol can be used as a carbon source. In the yeast Yarrowia lipolytica, its assimilation is activated by the transcription factor Euf1. The study investigates whether this factor can link erythritol to other processes in the cell.
Results
The research was performed on two closely related strains of Y. lipolytica: MK1 and K1, where strain K1 has no functional Euf1. Cultures were carried out in erythrol-containing and erythrol-free media. Transcriptome analysis revealed the effect of Euf1 on the regulation of more than 150 genes. Some of these could be easily connected with different aspects of erythritol assimilation, such as: utilization pathway, a new potential isoform of transketolase, or polyol transporters. However, many of the upregulated genes have never been linked to metabolism of erythritol. The most prominent examples are the degradation pathway of branched-chain amino acids and the glyoxylate cycle. The high transcription of genes affected by Euf1 is still dependent on the erythritol concentration in the medium. Moreover, almost all up-regulated genes have an ATGCA motif in the promoter sequence.
Conclusions
These findings are particularly relevant given the increasing use of erythritol-induced promoters in genetic engineering of Y. lipolytica. Moreover, use of this yeast in biotechnological processes often takes place under osmotic stress conditions. Erythritol might be produce as a by-product, thus better understanding of its influence on cell metabolism could facilitate processes optimization.
Research status and development trends of post-myocardial infarction heart failure: a bibliometric analysis from 2012 to 2022
Authors: Hou Chengzhi , Wang Jie , He Haoqiang , Liu Chao , Chen Cong , Huang Xuanchun , Jiang Zhilin , Cheng Jiawen
Published: January 2024

Objective: Post-myocardial infarction heart failure (post-MI HF) is a prevalent cardiovascular disease. The study aims to preliminarily explore the current research status and development trends in the field of post-MI HF, providing reference ideas and methods for clinical and scientific research.
Methods: Publications related to post-MI HF from 2012-2022 in the Web of Science Core Collection's SCI-expanded dataset were extracted. Bibliometric analysis was executed using VOSviewer and R-bibliometrix, encompassing the number of publications, countries, institutions, authors, journals, cited references, and keywords.
Results: Within the last decade, 10,929 publications in the domain were published, involving 58,357 authors from 10,437 research institutions across 120 different countries and regions. These findings appeared in 1,446 distinct journals. The United States led in the number of publications, with Harvard Medical School being the most active institution and Kober, Lars from the University of Copenhagen, Denmark, as the leading author. Plos One was the most prolific journal, while Circulationhad the highest citation count. Over the past decade, research hotspots mainly centered on mortality, cardiac remodeling, and prognosis. In the recent two years, emphasis shifted to topics such as mendelian randomization, dapagliflozin, sacubitril/valsartan, COVID-19, and machine learning.
Conclusion: The study reveals the overall performance of publications in the post-MI HF research field. The clinical prognosis of patients and cardiac remodeling have been research hotspots over the past decade, yet mortality and readmission remain urgent clinical challenges. Future research trends in this field may revolve around the mechanisms of cardiac remodeling, apoptosis, and inflammation, as well as clinical prognosis studies on patient mortality rates. Additionally, echocardiography and cardiac magnetic resonance technology are gradually becoming important methods for evaluating drug efficacy and elucidating related mechanisms.
Ångstrom-scale gold particles loaded with alendronate via alpha- lipoic acid alleviate bone loss in osteoporotic mice
Authors: Gao Weihang , Li Jiao Jiao , Shi Jingyu , Lan Hongbing , Guo Yuanyuan , Fu Dehao
Published: January 2024

Osteoporosis is a highly prevalent metabolic disease characterized by low systemic bone mass and deterioration of bone microarchitecture, resulting in reduced bone strength and increased fracture risk. Current treatment options for osteoporosis are limited by factors such as efficacy, cost, availability, side effects, and acceptability to patients. Gold nanoparticles show promise as an emerging osteoporosis therapy due to their osteogenic effects and ability to allow therapeutic delivery but have inherent constraints, such as low specificity and the potential for heavy metal accumulation in the body. This study reports the synthesis of ultrasmall gold particles almost reaching the Ångstrom (Ång) dimension. The antioxidant alpha-lipoic acid (LA) is used as a dispersant and stabilizer to coat Ångstrom-scale gold particles (AuÅPs). Alendronate (AL), an amino-bisphosphonate commonly used in drug therapy for osteoporosis, is conjugated through LA to the surface of AuÅPs, allowing targeted delivery to bone and enhancing antiresorptive therapeutic effects. In this study, alendronate-loaded Ångstrom-scale gold particles (AuÅPs-AL) were used for the first time to promote osteogenesis and alleviate bone loss through regulation of the WNT signaling pathway, as shown through in vitro tests. The in vivo therapeutic effects of AuÅPs-AL were demonstrated in an established osteoporosis mouse model. Microcomputed tomography, histology, and tartrate-resistant acid phosphatase staining indicated that AuÅPs-AL significantly improved bone density and prevented bone loss, with no evidence of nanoparticle-associated toxicity. These findings suggest the possible future application of AuÅPs-AL in osteoporosis therapy and point to the potential of developing new approaches for treating metabolic bone diseases using Ångstrom-scale gold particles.
Jian Gan powder ameliorates immunological liver injury in mice by modulating the gut microbiota and metabolic profiles
Authors: Li Kun , Cui Yadong , Zheng Xue , Min Chunyan , Zhang Jian , Yan Zhanpeng , Ji Yu , Ge Fei , Ji Hualiang , Zhu Fangshi
Published: January 2024

Objective
Immunological liver injury (ILI) is a common liver disease associated with the microbiota-gut-liver axis. Jian Gan powder (JGP) has protective and therapeutic effects on hepatitis virus-induced ILI in the clinic; nonetheless, underlying mechanisms are unclear.We aim to investigate the hepatoprotective effect of JGP in a mouse model of ILI.
Methods
The mouse model was established using Bacillus Calmette-Guérin (BCG) plus lipopolysaccharide (LPS). After JGP treatment (7.5, 15, or 30 g/kg), serum, liver, and fresh fecal samples were analyzed. 16S rRNA gene sequencing and untargeted metabolomics profiling were performed to assess the role of JGP on the gut microbiota and its metabolites.
Results
JGP treatment markedly reduced serum IFN-γ, IL-6, IL-22, and hepatic p-STAT3 (phosphorylated transducer and activator of transcription-3) expression. In contrast, JGP increased the percentage of proliferating cell nuclear antigen-positive liver cells in treated mice. Fecal 16S rRNA gene sequencing revealed that JGP treatment restored the levels of Alloprevotella, Burkholderia-Caballeronia-Paraburkholderia, Muribaculum, Streptococcus, and Stenotrophomonas. Additionally, metabolomics analysis of fecal samples showed that JGP restored the levels of allylestrenol, eplerenone, phosphatidylethanolamine (PE) (P-20:0/0:0), sphingomyelin (SM) d27:1, soyasapogenol C, chrysin, and soyasaponin I.
Conclusions
JGP intervention improves ILI by restoring gut microbiota and modifying its metabolic profiles. These results provide a novel insight into the mechanism of JGP in treating ILI and the scientific basis to support its clinical application.
Antibiotic prophylaxis during dental implant surgery treatment in Northwest China: A cross-sectional study
Authors: Lin Yao , Huang Jing , Yue Jiao , Cheng Lifei , Ma Yanfei , Pan Wen , Ju Jia , Wei Hongbo , Feng Bin
Published: January 2024

Background This study was designed to investigate the existing status of antibiotic prophylaxis during dental implant surgery in northwest China. Methods This cross-sectional study was conducted based on a web survey following the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines. The questionnaire comprised the basic information of respondents, the awareness of antibiotic prophylaxis and the antimicrobial prescription habits of dentists performing dental implants. The questionnaire was sent individually to 2099 dentists who performed dental implants in northwest China from April to December of 2021. Data were collected and analyzed. Results A total of 318 participants responded survey. Of the respondents, 65.41% (n = 208) routinely prescribed antibiotics during dental implant surgery, while only 0.94% (n = 3) did not prescribe them at all. A total of 141 (44.40%) respondents reported concerns regarding the indications for antibiotic use. The most frequently used antibiotics included penicillins, cephalosporins, and nitroimidazoles. Respondents inclined to prolong the regimens of antibiotics routinely for more than 24 h, specifically in patients with bone grafts,which reached to 85.08%. Conclusions Antibiotics are widely used to prevent infection during dental implant surgery in northwest China. Dentists are inclined to prolong the course of antibiotics routinely both in healthy individuals and patients with bone grafts.
Strength properties and water retention curve of unsaturated Berea sandstone during drying
Authors: Togashi Yota , Kosunago Yuki , Osada Masahiko , Asamoto Shingo , Hatakeyama Ken
Published: January 2024

A decrease in rock strength due to water content increase and water transfer in them must be considering while discussing the deformation of rock slopes and tunnels.A water retention curve is necessary to describe the strength properties and water transport for unsaturated rocks.Although the wetting curve can be obtained through mercury intrusion porosimetry test, generating the drying curve is difficult owing to the large suction. It is preferable to be able to identify the drying suctions in other ways.In this study, strength changes of Berea sandstone during drying due to water content change are shown,and a method for obtaining the water retention drying curve using strengths is presented. The degree of saturation in the specimens for both tests was was adjusted during the drying process by air drying in our laboratory room.The Mohr--Coulomb failure criteria were then obtained from the Brazilian and uniaxial compression test results. The results demonstrated that the shear resistance angle was almost constant regardless of the water content (degree of saturation), and only the cohesion decreased with increase in water content.Since this indicates the change in mean stress (suction) due to water content changes, suction can be identified from the Brazilian and uniaxial compression tests.Furthermore, Bayesian estimation of the van Genuchten parameters was performed using the suction determined by mean stress changes detected by series of loading tests. As expected, the obtained drying curve was larger than the wetting curve obtained by mercury intrusion porosimetry test.
Study on the asymmetric distribution characteristics of striatal dopamine transporter in early Parkinson's disease
Authors: Li Xiuming , Wang Ruifang , Huang Qi , Tan Haibo
Published: January 2024

Background
To exploring the characteristics of asymmetric distribution of dopamine transporter(DAT) in the striatum of early Parkinson's disease (PD) patients using 11C-2β-carbomethoxy-3β-(4-fluorophenyl)-tropane (11C-CFT) positron emission tomography/computed tomography (PET/CT) imaging.
Methods
We recruited 20 patients with early PD and 12 age- and gender-matched healthy controls for 11C-CFT PET/CT imaging. For each subject, the striatum was displayed on the maximum slice of 11C-CFT PET/CT images. Volumes of interest (VOIs) were manually outlined on the caudate, anterior and posterior putamen, and occipital cortex. The specific binding ratio (SBR), asymmetry index (AI), and the anterior-posterior gradient of striatal DAT uptake were calculated based on the mean standardized uptake value (SUVmean) within each VOI.
Results
The SBR of DAT in the anterior and posterior putamen, as well as in the bilateral caudate, was significantly lower in the early PD patients compared to the control group, especially on the contralateral side (p < 0.05). Compared to the control group, the asymmetry index and the anterior-posterior gradient of striatal DAT uptake were significantly higher in the striatal subregions of early PD patients, with the posterior putamen showing the greatest reduction, followed by the anterior putamen, and finally the caudate, indicating a trend of gradual development from posterior to anterior.
Conclusions
11C-CFT PET imaging can accurately demonstrate the characteristics of presynaptic dopaminergic neuron dysfunction in the striatum of early PD patients, providing imaging basis for the diagnosis and pathogenesis research of early PD.
The epigenomic consequences of HDAC6 inactivation reveal a crucial role for P300 suggesting integrated epitherapeutic strategies
Authors: Zamperla Michela Gottardi , Illi Barbara , Barbi Veronica , Cencioni Chiara , Santoni Daniele , Gagliardi Stella , Garofalo Maria , Zingale Gabriele Antonio , Pandino Irene , Sbardella Diego , Cipolla Lina , Sabbioneda Simone , Farsetti Antonella , Ripamonti Chiara , Fossati Gianluca , Steinkühler Christian , Gaetano Carlo , Atlante Sandra
Published: January 2024

Background: Histone deacetylases (HDACs) play a pivotal role in gene regulation, DNA synthesis, and cellular metabolism, significantly influencing cancer development. Class IIb histone deacetylase 6 (HDAC6) is crucial in maintaining protein stability and regulating chromatin dynamics, with notable implications in cancer and immune responses. Despite this, the specific effects of HDAC6 inactivation on gene regulation and chromatin remodeling remain unclear due to the subtle phenotype changes following its genetic deactivation. This study delves into how inactivating HDAC6 affects the stabilization of lysine acetyltransferase P300 and the subsequent impacts on chromatin structure and function in cancer cells.
Methods & Results: Using the HDAC6 inhibitor ITF3756 and CRISPR/Cas9 gene editing, we deactivated HDAC6 in various cancer cell lines. We observed profound changes in chromatin accessibility, particularly in the acetylation of histone H3 lysines 9, 14, and 27, which accumulated into introns and distal intergenic regions as determined through ATAC-seq and H3K27Ac ChIP-seq analyses. Transcriptomics, proteomics, and gene ontology analysis revealed alterations in gene function linked to cell proliferation, adhesion, migration, and apoptosis. A notable finding was the modification of P300 ubiquitination post HDAC6 inactivation, which increased P300 expression and activity, resulting in the downregulation of genes essential for cellular proliferation and survival.
Conclusions: This research underscores the significant effect of HDAC6 inactivation on the chromatin landscape in cancer cells, shedding light on the crucial role of P300 in the HDAC6-mediated anticancer response. Specifically, the role of P300 stabilization in the effectiveness of an HDAC6 inhibitor suggests for a potential shift in focus from HDAC6 to its interaction with P300. This insight could contribute to developing more precise cancer treatments by targeting this specific pathway, thereby enhancing our knowledge of cancer cell behavior and offering new therapeutic perspectives.
Barriers and Facilitators to Health-seeking Behaviors and Self-care Practices of Older Adults in Rural India, A Mixed Method Study
Authors: M Yogesh , Makwana Naresh , Damor Naresh , Trivedi Nidhi
Published: January 2024

Background: Effective self-care is crucial for maintaining health in older adults in resource-constrained communities. This study examined self-care practices, health-seeking behaviors, and associated factors among older adults in rural India.
Methods: A mixed-methods study was conducted among 400 adults aged ≥65 years. Participants were selected through multistage random sampling from 5 slum areas. Self-care practices, health-seeking behaviors, demographic information, chronic conditions, self-efficacy, and health literacy were assessed through interviews. The qualitative data were explored through in-depth interviews with 30 participants.
Results: Inadequate health literacy 180 (45%) and low self-efficacy 150 (37.5%) were common. While 300 (75%) reported an adequate diet and 350 (87.5%) reported medication adherence, only 80 (20%) monitored diabetes complications. Only 180 (45%) patients underwent cancer screening, and 300 (75%) had ≥2 doctor visits yearly. Age, sex, education, income, comorbidities, self-efficacy, and health literacy were significantly associated with self-care and health-seeking. Along with facilitators such as social support, barriers such as limited healthcare access and lack of preventive orientation emerged.
Conclusion: Suboptimal prevention orientation and overreliance on secondary care instead of self-care among elderly people are problematic given the limited use of geriatric services. Grassroots health workers can improve health literacy and self-efficacy through home visits to enable self-care. Healthcare access inequities for vulnerable groups merit policy attention.
Functional identification of CCR1 gene in apple (Malus halliana) demonstrates that it enhances saline-alkali stress tolerance
Authors: Wang Xiu , Zhang Zhongxing , wang Wanxia , Li SiTian , Li JuanLi , wang Yanxiu
Published: January 2024

Background
Lignin is a complex aromatic polymer that plays an important biological role in maintaining plant structure and defending plants against biotic and abiotic stresses. Cinnamoyl-CoA reductase (CCR) is a key enzyme involved in the lignin synthesis-specific pathway and regulates lignin biosynthesis and accumulation.
Methods
Based on transcriptome data, MhCCR1, which was significantly induced by saline-alkali stress, was cloned from Malus halliana. The physicochemical properties, evolutionary relationships and cis-acting elements were analyzed. Subsequently, the tolerance of overexpressed MhCCR1 in Arabidopsis thaliana, tobacco and apple calli to saline-alkali stress was verified by genetic transformation. And yeast two-hybridization technique was applied to screen and validate the interacting proteins.
Results
We found that overexpression of MhCCR1 enhanced the tolerance of A. thaliana, tobacco and apple calli under saline-alkali stress, and caused a variety of physiological and biochemical changes. As compared to the wild type, the transgenic plants showed better growth, higher lignin, chlorophyll and proline contents, lower conductivity and MDA content, and significant increase in antioxidant enzyme activities (SOD, POD, CAT) in the transgenic lines under stress condition. In addition, expression of saline-alkali stress-related genes in overexpressed A. thaliana were also higher than in WT, including the antioxidant genes, the Na+ transporter genes, and the H+-ATPase genes, while expression of the K+ transporter genes displayed opposite changes. Meanwhile, the expression levels of genes related to lignin synthesis, AtPAL1, AtCOMT, AtC4H, At4CL1, and AtCCOAOMT, were also significantly up-regulated. At last, the Y2H experiment confirmed the interaction between MhCCR1 and MhMYB4, MhMYB1R1, MhHXK, and MhbZIP23 proteins.
Conclusions
These results suggest that MhCCR1 may play a positive regulatory role in saline-alkali tolerance of transgenic lines by regulating the lignin content, osmoregulatory substances, chlorophyll content, antioxidant enzyme activities, and genes related to saline-alkali stress, thus providing excellent resistance genes for the stress-responsive regulatory network of apples, and providing a theoretical basis for the cultivation of saline and alkali resistant apple varieties.
Synthesis, structure, and catalytic behaviour for oxygen reduction of a µ2-OH-bridged binuclear cobalt complex
Authors: Li Can-Hao , Li Wei , Zhan Shu-Zhong
Published: January 2024

The reaction of 2-pyridylamino-N,N-bis(2-methylene-4,6-dichlorophenol) (H2L) with CoCl2 affords a binuclear cobalt complex, [L-Co-µ2(OH)2-Co-L], which has been determined by using single crystal X-ray diffraction. Electrochemical studies show that this cobalt complex can serve as a molecular catalyst for oxygen reduction reaction (ORR). Under − 0.4 V vs Ag/AgCl, the H2O2 yield is 83% with 0.20 M KOH. Moreover, investigations exbibit that in homogeneous environments dissociation of a OH− ion from the binuclear cobalt complex makes positions on cobalt centres, letting dioxygen (O2) inserted and reduced. We hope these findings can provide a new ideal for the developments of electrocatalysts for ORR.