bluuebunny/crossref_metadata_2025 huggingface dataset:
Stimulation of GABA Receptors in the Lateral Septum Rapidly Elicits Food Intake and Mediates Natural Feeding
Authors: Gabriella Ivett , Tseng Andy , Sanchez Kevin O. , Shah Himani , Stanley Billy Glenn
Published: June 2022

The increasing prevalence of obesity and eating disorders makes identifying neural substrates controlling eating and regulating body weight a priority. Recent studies have highlighted the role of the lateral septum (LS) in eating control mechanisms. The current study explored the roles of gamma-aminobutyric acid (GABA) receptors within the LS in the control of food intake. Experiments with a rat model (n ≥ 11/group) showed that LS microinjection of the GABAA receptor agonist, muscimol, and the GABAB receptor agonist, baclofen hydrochloride (baclofen), elicited intense, dose-dependent feeding. In contrast, LS pretreatment with the GABAA receptor antagonist, picrotoxin, markedly reduced the muscimol-elicited feeding, and pretreatment injections with the GABAB receptor antagonist, 2-hydroxysaclofen (2-OH saclofen), reduced the baclofen evoked response. Next, we showed that picrotoxin injection at the beginning of the dark phase of the light-dark cycle—when rats show a burst of spontaneous eating—reduced naturally occurring feeding, whereas 2-OH saclofen was ineffective. These results indicate that the activation of LS GABAA and GABAB receptors strongly stimulates feeding and suggests potential roles in feeding control neurocircuitry. In particular, our evidence indicates that endogenous LS GABA and GABAA receptors may be involved in mediating naturally occurring nocturnal feeding.
Diffusion Tensor Imaging Radiomics for Diagnosis of Parkinson’s Disease
Authors: Li Jingwen , Liu Xiaoming , Wang Xinyi , Liu Hanshu , Lin Zhicheng , Xiong Nian
Published: June 2022

Background: Diagnosis of Parkinson’s Disease (PD) based on clinical symptoms and scale scores is mostly objective, and the accuracy of neuroimaging for PD diagnosis remains controversial. This study aims to introduce a radiomic tool to improve the sensitivity and specificity of diagnosis based on Diffusion Tensor Imaging (DTI) metrics. Methods: In this machine learning-based retrospective study, we collected basic clinical information and DTI images from 54 healthy controls (HCs) and 56 PD patients. Among them, 60 subjects (30 PD patients and 30 HCs) were assigned to the training group, whereas the test cohort was 26 PD patients and 24 HCs. After the feature extraction and selection using newly developed image processing software Ray-plus, LASSO regression was used to finalize radiomic features. Results: A total of 4600 radiomic features were extracted, of which 12 were finally selected. The values of the AUC (area under the subject operating curve) in the training group, the validation group, and overall were 0.911, 0.931, and 0.919, respectively. Conclusion: This study introduced a novel radiometric and computer algorithm based on DTI images, which can help increase the sensitivity and specificity of PD screening.
Mindfulness Meditation as Psychosocial Support in the Breast Cancer Experience: A Case Report
Authors: Iannopollo Letizia , Cristaldi Grazia , Borgese Caterina , Sommacal Samuela , Silvestri Giulia , Serpentini Samantha
Published: June 2022

In the last decade, Mindfulness-based interventions have been increasingly used in health care settings, particularly in the context of cancer. Research documents the efficacy of these interventions for decreasing the burdens of stress, anxiety, depression, fatigue, sleep disorders, and other symptoms. This article describes the case report of a patient with breast cancer, highlighting her personality, defense mechanisms, and traumatization connected with the disease. General information about the patient’s personal and medical history is presented in addition to the trajectory of psychoncological support, focusing on objectives, intervention strategies based on Mindfulness, and outcomes. The intervention is a combination of individual and group therapies, with particular reference to the use of Mindfulness in a group setting. The goal is to provide the patient with both a peer sharing experience as well as the tools to manage psychoemotional reactions through the development of awareness and a better relationship with herself. The main hypothesized consequences are an increase in self-esteem and coping strategies, which are necessary for a successful adaptation to cancer. The objective of the Mindfulness intervention is to promote the maintenance of an adequate Quality of Life (QoL) and psychological well-being, during and after treatment, transferring these skills into daily life.
Thermal Environment and Thermal Comfort in University Classrooms during the Heating Season
Authors: Zhang Jiuhong , Li Peiyue , Ma Mingxiao
Published: June 2022

In recent years, there has been increasing concern about the effects of indoor thermal environments on human physical and mental health. This paper aimed to study the current status of the thermal environment and thermal comfort in the classrooms of Northeastern University during the heating season. The indoor thermal environment was analyzed with the use of field measurements, a subjective questionnaire, regression statistics, and the entropy weight method. The results show that personnel population density is an important factor affecting the temperature and relative humidity variations in classrooms. The results also show that the temperature and relative humidity in a lecture state are respectively 4.2 °C and 11.4% higher than those in an idle state. In addition, in university classrooms in Shenyang, the actual thermal neutral temperature is 2.5 °C lower than the predicted value of the Predicted Mean Vote. It was found that increasing indoor relative humidity can effectively improve the overall thermal comfort of subjects. Furthermore, the temperature preference of women was higher than that of men. Therefore, when setting the initial heating temperature, the personnel population density and sufficient indoor relative humidity have been identified as the key factors for improving the thermal environment of the classroom.
Integrative Analysis of Intrahepatic Cholangiocarcinoma Subtypes for Improved Patient Stratification: Clinical, Pathological, and Radiological Considerations
Authors: Gerber Tiemo S. , Müller Lukas , Bartsch Fabian , Gröger Lisa-Katharina , Schindeldecker Mario , Ridder Dirk A. , Goeppert Benjamin , Möhler Markus , Dueber Christoph , Lang Hauke , Roth Wilfried , Kloeckner Roman , Straub Beate K.
Published: June 2022

Intrahepatic cholangiocarcinomas (iCCAs) may be subdivided into large and small duct types that differ in etiology, molecular alterations, therapy, and prognosis. Therefore, the optimal iCCA subtyping is crucial for the best possible patient outcome. In our study, we analyzed 148 small and 84 large duct iCCAs regarding their clinical, radiological, histological, and immunohistochemical features. Only 8% of small duct iCCAs, but 27% of large duct iCCAs, presented with initial jaundice. Ductal tumor growth pattern and biliary obstruction were significant radiological findings in 33% and 48% of large duct iCCAs, respectively. Biliary epithelial neoplasia and intraductal papillary neoplasms of the bile duct were detected exclusively in large duct type iCCAs. Other distinctive histological features were mucin formation and periductal-infiltrating growth pattern. Immunohistochemical staining against CK20, CA19-9, EMA, CD56, N-cadherin, and CRP could help distinguish between the subtypes. To summarize, correct subtyping of iCCA requires an interplay of several factors. While the diagnosis of a precursor lesion, evidence of mucin, or a periductal-infiltrating growth pattern indicates the diagnosis of a large duct type, in their absence, several other criteria of diagnosis need to be combined.
Urinary microRNAs and Their Significance in Prostate Cancer Diagnosis: A 5-Year Update
Authors: Juracek Jaroslav , Madrzyk Marie , Stanik Michal , Slaby Ondrej
Published: June 2022

Current routine screening methods for the diagnosis of prostate cancer (PCa) have significantly increased early detection of the disease but often show unsatisfactory analytical parameters. A class of promising markers represents urinary microRNAs (miRNAs). In the last five years, there has been an extensive increase in the number of studies on this topic. Thus, this review aims to update knowledge and point out technical aspects affecting urinary miRNA analysis. The review of relevant literature was carried out by searching the PubMed database for the keywords: microRNA, miRNA, urine, urinary, prostate cancer, and diagnosis. Papers discussed in this review were retrieved using PubMed, and the search strategy was as follows: (urine OR urinary) WITH (microRNA OR miRNA) AND prostate cancer. The search was limited to the last 5 years, January 2017 to December 2021. Based on the defined search strategy, 31 original publications corresponding to the research topic were identified, read and reviewed to present the latest findings and to assess possible translation of urinary miRNAs into clinical practice. Reviews or older publications were read and cited if they valuably extended the context and contributed to a better understanding. Urinary miRNAs are potentially valuable markers for the diagnosis of prostate cancer. Despite promising results, there is still a need for independent validation of exploratory data, which follows a strict widely accepted methodology taking into account the shortcomings and factors influencing the analysis.
Genomic Markers of CDK 4/6 Inhibitor Resistance in Hormone Receptor Positive Metastatic Breast Cancer
Authors: Lee Jin Sun , Yost Susan E. , Li Sierra Min , Cui Yujie , Frankel Paul H. , Yuan Yate-Ching , Schmolze Daniel , Egelston Colt A. , Guo Weihua , Murga Mireya , Chang Helen , Bosserman Linda , Yuan Yuan
Published: June 2022

Cyclin-dependent kinase 4/6 inhibitors are the standard of care for hormone receptor-positive metastatic breast cancer. This retrospective study reports on genomic biomarkers of CDK 4/6i resistance utilizing genomic data acquired through routine clinical practice. Patients with HR+ MBC treated with palbociclib, ribociclib, or abemaciclib and antiestrogen therapy were identified. Patients were grouped into early (<6 months); intermediate (6–24 months for 0–1 lines; 6–9 months for ≥2 lines); or late progressors (>24 months for 0–1 lines; >9 months PFS for ≥2 lines). NGS and RNA sequencing data were analyzed in association with PFS, and survival analysis was stratified by prior lines of chemotherapy. A total of 795 patients with HR+ MBC treated with CDK 4/6i were identified. Of these, 144 (18%) patients had genomic data and 29 (3.6%) had RNA data. Among the 109 patients who received CDK4/6i as 1st- or 2nd-line therapy, 17 genes showed associations with PFS (p-value ≤ 0.15 and HR ≥ 1.5 or HR < 0.5). Whole transcriptome RNAseq was analyzed for 24/109 (22%) patients with 0–1 prior lines of therapy and 56 genes associated with PFS (HR ≥ 4 or HR ≤ 0.25 and FDR ≤ 0.15). In this retrospective analysis, genomic biomarkers including FGFR1 amplification, PTEN loss, and DNA repair pathway gene mutations showed significant associations with shorter PFS for patients receiving CDK4/6 inhibitor therapy.
WNT/β-Catenin-Mediated Resistance to Glucose Deprivation in Glioblastoma Stem-like Cells
Authors: Yusuf Suad , Aretz Philippe , Nickel Ann-Christin , Westhoff Philipp , Sharma Amit , Qin Nan , Remke Marc , Steiger Hans-Jakob , Hänggi Daniel , Liu Hongjia , Liu Hongde , Neumann Silke , Reifenberger Guido , Maciaczyk Jarek
Published: June 2022

Isocitrate dehydrogenase (IDH)-wildtype glioblastoma is the most common primary malignant brain tumor. It is associated with a particularly poor prognosis, as reflected by an overall median survival of only 15 months in patients who undergo a supramarginal surgical reduction of the tumor mass followed by combined chemoradiotherapy. The highly malignant nature of IDH-wildtype glioblastoma is thought to be driven by glioblastoma stem-like cells (GSCs) that harbor the ability of self-renewal, survival, and adaptability to challenging environmental conditions. The wingless (WNT) signaling pathway is a phylogenetically highly conserved stemness pathway, which promotes metabolic plasticity and adaptation to a nutrient-limited tumor microenvironment. To unravel the reciprocal regulation of the WNT pathway and the nutrient-limited microenvironment, glioblastoma cancer stem-like cells were cultured in a medium with either standard or reduced glucose concentrations for various time points (24, 48, and 72 h). Glucose depletion reduced cell viability and facilitated the survival of a small population of starvation-resistant tumor cells. The surviving cells demonstrated increased clonogenic and invasive properties as well as enhanced chemosensitivity to pharmacological inhibitors of the WNT pathway (LGK974, berberine). Glucose depletion partially led to the upregulation of WNT target genes such as CTNNB1, ZEB1, and AXIN2 at the mRNA and corresponding protein levels. LGK974 treatment alone or in combination with glucose depletion also altered the metabolite concentration in intracellular compartments, suggesting WNT-mediated metabolic regulation. Taken together, our findings suggest that WNT-mediated metabolic plasticity modulates the survival of GSCs under nutrient-restricted environmental conditions.
Specialist versus Primary Care Prostate Cancer Follow-Up: A Process Evaluation of a Randomized Controlled Trial
Authors: Wollersheim Barbara M. , van Asselt Kristel M. , Pos Floris J. , Akdemir Emine , Crouse Shifra , van der Poel Henk G. , Aaronson Neil K. , van de Poll-Franse Lonneke V. , Boekhout Annelies H.
Published: June 2022

Background: A randomized controlled trial (RCT) is currently comparing the effectiveness of specialist- versus primary care-based prostate cancer follow-up. This process evaluation assesses the reach and identified constructs for the implementation of primary care-based follow-up. Methods: A mixed-methods approach is used to assess the reach and the implementation through the Consolidated Framework for Implementation Research. We use quantitative data to evaluate the reach of the RCT and qualitative data (interviews) to indicate the perspectives of patients (n = 15), general practitioners (GPs) (n = 10), and specialists (n = 8). Thematic analysis is used to analyze the interview transcripts. Results: In total, we reached 402 (67%) patients from 12 hospitals and randomized them to specialist- (n = 201) or to primary care-based (n = 201) follow-up. From the interviews, we identify several advantages of primary care- versus specialist-based follow-up: it is closer to home, more accessible, and the relationship is more personal. Nevertheless, participants also identified challenges: guidelines should be implemented, communication and collaboration between primary and secondary care should be improved, quality indicators should be collected, and GPs should be compensated. Conclusion: Within an RCT context, 402 (67%) patients and their GPs were willing to receive/provide primary care-based follow-up. If the RCT shows that primary care is equally as effective as specialist-based follow-up, the challenges identified in this study need to be addressed to enable a smooth transition of prostate cancer follow-up to primary care.
Multiplex Tissue Imaging: Spatial Revelations in the Tumor Microenvironment
Authors: van Dam Stephanie , Baars Matthijs J. D. , Vercoulen Yvonne
Published: June 2022

The tumor microenvironment is a complex ecosystem containing various cell types, such as immune cells, fibroblasts, and endothelial cells, which interact with the tumor cells. In recent decades, the cancer research field has gained insight into the cellular subtypes that are involved in tumor microenvironment heterogeneity. Moreover, it has become evident that cellular interactions in the tumor microenvironment can either promote or inhibit tumor development, progression, and drug resistance, depending on the context. Multiplex spatial analysis methods have recently been developed; these have offered insight into how cellular crosstalk dynamics and heterogeneity affect cancer prognoses and responses to treatment. Multiplex (imaging) technologies and computational analysis methods allow for the spatial visualization and quantification of cell–cell interactions and properties. These technological advances allow for the discovery of cellular interactions within the tumor microenvironment and provide detailed single-cell information on properties that define cellular behavior. Such analyses give insights into the prognosis and mechanisms of therapy resistance, which is still an urgent problem in the treatment of multiple types of cancer. Here, we provide an overview of multiplex imaging technologies and concepts of downstream analysis methods to investigate cell–cell interactions, how these studies have advanced cancer research, and their potential clinical implications.
Preferential Antibody and Drug Conjugate Targeting of the ADAM10 Metalloprotease in Tumours
Authors: Yan Hengkang , Vail Mary E. , Hii Linda , Guo Nancy , McMurrick Paul J. , Oliva Karen , Wilkins Simon , Saha Nayanendu , Nikolov Dimitar B. , Lee Fook-Thean , Scott Andrew M. , Janes Peter W.
Published: June 2022

ADAM10 is a transmembrane metalloprotease that sheds a variety of cell surface proteins, including receptors and ligands that regulate a range of developmental processes which re-emerge during tumour development. While ADAM10 is ubiquitously expressed, its activity is normally tightly regulated, but becomes deregulated in tumours. We previously reported the generation of a monoclonal antibody, 8C7, which preferentially recognises an active form of ADAM10 in human and mouse tumours. We now report our investigation of the mechanism of this specificity, and the preferential targeting of 8C7 to human tumour cell xenografts in mice. We also report the development of novel 8C7 antibody–drug conjugates that preferentially kill cells displaying the 8C7 epitope, and that can inhibit tumour growth in mice. This study provides the first demonstration that antibody–drug conjugates targeting an active conformer of ADAM10, a widely expressed transmembrane metalloprotease, enable tumour-selective targeting and inhibition.
Effect of Antibacterial Prophylaxis on Febrile Neutropenic Episodes and Bacterial Bloodstream Infections in Dutch Pediatric Patients with Acute Myeloid Leukemia: A Two-Center Retrospective Study
Authors: Van Weelderen Romy E. , Klein Kim , Goemans Bianca F. , Tissing Wim J. E. , Wolfs Tom F. W. , Kaspers Gertjan J. L.
Published: June 2022

Bloodstream infections (BSIs), especially those caused by Gram-negative rods (GNR) and viridans group streptococci (VGS), are common and potentially life-threatening complications of pediatric acute myeloid leukemia (AML) treatment. Limited literature is available on prophylactic regimens. We retrospectively evaluated the effect of different antibacterial prophylaxis regimens on the incidence of febrile neutropenic (FN) episodes and bacterial BSIs. Medical records of children (0–18 years) diagnosed with de novo AML and treated at two Dutch centers from May 1998 to March 2021 were studied. Data were analyzed per chemotherapy course and consecutive neutropenic period. A total of 82 patients had 316 evaluable courses: 92 were given with single-agent ciprofloxacin, 138 with penicillin plus ciprofloxacin, and 51 with teicoplanin plus ciprofloxacin. The remaining 35 courses with various other prophylaxis regimens were not statistically compared. During courses with teicoplanin plus ciprofloxacin, significantly fewer FN episodes (43 vs. 90% and 75%; p < 0.0001) and bacterial BSIs (4 vs. 63% and 33%; p < 0.0001) occurred than with single-agent ciprofloxacin and penicillin plus ciprofloxacin, respectively. GNR and VGS BSIs did not occur with teicoplanin plus ciprofloxacin and no bacterial BSI-related pediatric intensive care unit (PICU) admissions were required, whereas, with single-agent ciprofloxacin and penicillin plus ciprofloxacin, GNR BSIs occurred in 8 and 1% (p = 0.004), VGS BSIs in 24 and 14% (p = 0.0005), and BSI-related PICU admissions were required in 8 and 2% of the courses (p = 0.029), respectively. Teicoplanin plus ciprofloxacin as antibacterial prophylaxis is associated with a lower incidence of FN episodes and bacterial BSIs. This may be a good prophylactic regimen for pediatric AML patients during treatment.
Analysis of the Clinical Advancements for BRCA-Related Malignancies Highlights the Lack of Treatment Evidence for BRCA-Positive Male Breast Cancer
Authors: McClurg Dylan P. , Urquhart Gordan , McGoldrick Trevor , Chatterji Subarnarekha , Miedzybrodzka Zosia , Speirs Valerie , Elsberger Beatrix
Published: June 2022

Male breast cancer (MBC) is a rare disease that accounts for less than 1% of all breast cancers and male malignancies. Despite recognised clinico-pathological and molecular differences to female breast cancer (FBC), the clinical management of MBC follows established FBC treatment strategies. Loss of function mutations in the DNA damage response genes BRCA1 and BRCA2, have been strongly implicated in the pathogenesis of MBC. While there have been extensive clinical advancements in other BRCA-related malignancies, including FBC, improvements in MBC remain stagnant. Here we present a review that highlights the lack of treatment evidence for BRCA-related MBC and the required national and global collaborative effort to address this unmet need. In doing so, we summarise the transformative clinical advancements with poly(ADP-ribose) polymerase (PARP) inhibitors in other BRCA-related cancers namely, FBC and prostate cancer.
Robot-Assisted Minimally Invasive Esophagectomy versus Open Esophagectomy for Esophageal Cancer: A Systematic Review and Meta-Analysis
Authors: Esagian Stepan M. , Ziogas Ioannis A. , Skarentzos Konstantinos , Katsaros Ioannis , Tsoulfas Georgios , Molena Daniela , Karamouzis Michalis V. , Rouvelas Ioannis , Nilsson Magnus , Schizas Dimitrios
Published: June 2022

Robot-assisted minimally invasive esophagectomy (RAMIE) was introduced as a further development of the conventional minimally invasive esophagectomy, aiming to further improve the high morbidity and mortality associated with open esophagectomy. We aimed to compare the outcomes between RAMIE and open esophagectomy, which remains a popular approach for resectable esophageal cancer. Ten studies meeting our inclusion criteria were identified, including five retrospective cohort, four prospective cohort, and one randomized controlled trial. RAMIE was associated with significantly lower rates of overall pulmonary complications (odds ratio (OR): 0.38, 95% confidence interval (CI): [0.26, 0.56]), pneumonia (OR: 0.39, 95% CI: [0.26, 0.57]), atrial fibrillation (OR: 0.53, 95% CI: [0.29, 0.98]), and wound infections (OR: 0.20, 95% CI: [0.07, 0.57]) and resulted in less blood loss (weighted mean difference (WMD): −187.08 mL, 95% CI: [−283.81, −90.35]) and shorter hospital stays (WMD: −9.22 days, 95% CI: [−14.39, −4.06]) but longer operative times (WMD: 69.45 min, 95% CI: [34.39, 104.42]). No other statistically significant difference was observed regarding surgical and short-term oncological outcomes. Similar findings were observed when comparing totally robotic procedures only to OE. RAMIE is a safe and feasible procedure, resulting in decreased cardiopulmonary morbidity, wound infections, blood loss, and shorter hospital stays compared to open esophagectomy.
Influence of the Use of Permanent Catalytic Systems on the Flue Gases Emission from Biomass Low-Power Boilers
Authors: Gaze Błażej , Knutel Bernard , Jajczyk Mateusz , Němček Ondřej , Najser Tomáš , Kielar Jan
Published: June 2022

The paper presents the research results on the use of permanent catalytic systems applied to the surface of a low-power boiler deflector. The tests were carried out on a standard 15 kW retort boiler. The boiler was powered by three types of biomass pellets (wood pellets, wheat straw pellets, and hemp expeller). In the research cycle, the influence of the catalysts on the emission of individual compounds, CO, NOX, particulate matter (PM), polycyclic aromatic hydrocarbons (PAH), and volatile organic compounds (VOC) and the influence on the temperature in the combustion chamber were examined. The tests used an exhaust gas analyzer, a dust meter, a two-channel aspirator, and a laboratory gas chromatograph stand with a flame ionization detector. Four catalysts (copper, manganese, titanium, and platinum) were prepared for the analysis. Each catalyst had three variants of the active substance concentration on the ceramic support surface: 17.5 g, 35 g, 52.5 g for CuO, TiO2, MnO2, and, respectively, 0.05 g, 0.1 g, and 0.15 g for platinum. Concerning the deflector surface, this concentration corresponded to 140, 280, and 420 g·m−2 for CuO, TiO2, and MnO2, and 0.4, 0.8, and 1.2 g·m−2 for platinum catalysts. All the catalysts used contributed to an increase in the combustion temperature and a reduction in pollutant emissions. The results presented in the paper will allow the implementation of the developed solutions in the industry producing low-power boilers and in already-existing heating installations. The factor that motivates the introduction of changes may be continuously tightening European emission regulations.
Combining Ultraviolet Photolysis with In-Situ Electrochemical Oxidation for Degrading Sulfonamides in Wastewater
Authors: Zheng Zhijie , Yuan Julin , Jiang Xinwei , Han Gang , Tao Yufang , Wu Xiaogang
Published: June 2022

Ultraviolet photolysis (UVC, 254 nm) was coupled with an electrochemical oxidation process to degrade three kinds of veterinary sulfonamide (sulfamethazine [SMZ] tablets, sulfamonomethoxine [SMM] tablets, and compound sulfamethoxazole [SMX] tablets). The treatment was applied using a flat ceramic microfiltration membrane to study the effects of photocatalysts. The effectiveness of degradation of the three sulfonamides was evaluated under different conditions. Dissolved oxygen was provided via aeration, but this resulted in a large decrease in the degradation effectiveness due to the inhibition of free chlorine electrogeneration. The photocatalysts had no promotional effect on sulfonamide removal from wastewater due to reduced UV penetration. Because of the different distribution coefficients of sulfonamides, UV irradiation had different effects on different sulfonamide species. For SMZ and SMM, anionic species exhibited a higher degradation rate, whereas for SMX, degradation was most effective for neutral species. In addition, the free chlorine yield increased as the pH increased. Free chlorine conversion reactions occurred under UV irradiation, with the reactions possibly restrained by sulfonamides. Reactive chlorine species promoted SMM degradation. Compared to UV irradiation or electrochemical oxidation alone, the UV/in-situ electrochemical oxidation process was more effective and is suitable for treating real wastewater under various environmental pH levels.
Cancer Stem Cell-Associated Immune Microenvironment in Recurrent Glioblastomas
Authors: Murota Yoshitaka , Tabu Kouichi , Taga Tetsuya
Published: June 2022

Glioblastoma multiforme (GBM) is the most incurable tumor (due to the difficulty in complete surgical resection and the resistance to conventional chemo/radiotherapies) that displays a high relapse frequency. Cancer stem cells (CSCs) have been considered as a promising target responsible for therapy resistance and cancer recurrence. CSCs are known to organize a self-advantageous microenvironment (niche) for their maintenance and expansion. Therefore, understanding how the microenvironment is reconstructed by the remaining CSCs after conventional treatments and how it eventually causes recurrence should be essential to inhibit cancer recurrence. However, the number of studies focusing on recurrence is limited, particularly those related to tumor immune microenvironment, while numerous data have been obtained from primary resected samples. Here, we summarize recent investigations on the immune microenvironment from the viewpoint of recurrent GBM (rGBM). Based on the recurrence-associated immune cell composition reported so far, we will discuss how CSCs manipulate host immunity and create the special microenvironment for themselves to regrow. An integrated understanding of the interactions between CSCs and host immune cells at the recurrent phase will lead us to develop innovative therapies and diagnoses to achieve GBM eradication.
Drosophila MESR4 Gene Ensures Germline Stem Cell Differentiation by Promoting the Transcription of bag of marbles
Authors: Szarka-Kovács Alexandra Brigitta , Takács Zsanett , Bence Melinda , Erdélyi Miklós , Jankovics Ferenc
Published: June 2022

Ovarian germline stem cells (GSCs) of Drosophila melanogaster provide a valuable in vivo model to investigate how the adult stem cell identity is maintained and the differentiation of the daughter cells is regulated. GSCs are embedded into a specialized cellular microenvironment, the so-called stem cell niche. Besides the complex signaling interactions between the germ cells and the niche cells, the germ cell intrinsic mechanisms, such as chromatin regulation and transcriptional control, are also crucial in the decision about self-renewal and differentiation. The key differentiation regulator gene is the bag of marbles (bam), which is transcriptionally repressed in the GSCs and de-repressed in the differentiating daughter cell. Here, we show that the transcription factor MESR4 functions in the germline to promote GSC daughter differentiation. We find that the loss of MESR4 results in the accumulation of GSC daughter cells which fail to transit from the pre-cystoblast (pre-CB) to the differentiated cystoblast (CB) stage. The forced expression of bam can rescue this differentiation defect. By a series of epistasis experiments and a transcriptional analysis, we demonstrate that MESR4 positively regulates the transcription of bam. Our results suggest that lack of repression alone is not sufficient, but MESR4-mediated transcriptional activation is also required for bam expression.
ANNEXIN A1: Roles in Placenta, Cell Survival, and Nucleus
Authors: Sousa Stefanie Oliveira de , Santos Mayk Ricardo dos , Teixeira Samuel Cota , Ferro Eloisa Amália Vieira , Oliani Sonia Maria
Published: June 2022

The unbiased approaches of the last decade have enabled the collection of new data on the biology of annexin A1 (ANXA1) in a variety of scientific aspects, creating opportunities for new biomarkers and/or therapeutic purposes. ANXA1 is found in the plasma membrane, cytoplasm, and nucleus, being described at low levels in the nuclear and cytoplasmic compartments of placental cells related to gestational diabetic diseases, and its translocation from the cytoplasm to the nucleus has been associated with a response to DNA damage. The approaches presented here open pathways for reflection upon, and intrinsic clarification of, the modulating action of this protein in the response to genetic material damage, as well as its level of expression and cellular localization. The objective of this study is to arouse interest, with an emphasis on the mechanisms of nuclear translocation of ANXA1, which remain underexplored and may be beneficial in new inflammatory therapies.
Review—Recent Progress in Graphene Based Modified Electrodes for Electrochemical Detection of Dopamine
Authors: Hsine Zouhour , Mlika Rym , Jaffrezic-Renault Nicole , Korri-Youssoufi Hafsa
Published: June 2022

Graphene and its derivatives have been widely used for the electrochemical detection of dopamine (DA) neurotransmitter, thanks to its high surface area and excellent conductivity. Modified graphene and graphene-based nanocomposites have shown improved catalytic activity towards DA detection. Various modification approaches have been taken, including heteroatom doping and association with other nanomaterials. This review summarizes and highlights the recent advances in graphene-based electrodes for the electrochemical detection of DA. It also aims to provide an overview of the advantages of using polymer as a linker platform to form graphene-based nanocomposites applied to electrochemical DA sensors.
Primary School Pupils: Unequal GMC Developmental Pathways in a Single School Year
Authors: Niet Mark de , Wetzels Veerle M. A. , Pion Johan , Faber Irene R. , Platvoet Sebastiaan W. J. , Elferink-Gemser Marije T.
Published: June 2022

Gross motor coordination (GMC) is essential for the development of specific motor skills and long-term participation in physical activities and sports. Group analysis reveals that, on average, children develop these skills gradually; however, how individuals develop GMC is less clear. The main aim of this study is to increase the understanding of developmental patterns within one school year, and whether children’s grade, gender, or baseline GMC proficiency are associated with these developmental patterns. In total, 2594 Dutch children aged 6–12 years performed the modified Körper Koordinations Test für Kinder (KTK3+) twice in one school year (autumn and spring). The KTK3+ includes four subtests: walking backwards, moving sideways, jumping sideways, and eye-hand coordination (EHC) test. On average, children developed significantly on all subtests (p < 0.001). At baseline, children in higher grades scored significantly higher than children in lower grades, and children in grades 5 and 6 (age 9 and 10 years) showed most development (raw scores on average, p < 0.001). Boys outperformed girls on EHC across all grades, whereas girls outperformed boys on walking backwards. Nevertheless, both boys and girls developed similarly. Children with lower scores at baseline developed more quickly across all grades. Noteworthy is that 12.1–24% (depending on the test item) of the children scored lower in the spring than in the autumn tests. On average, children develop their GMC; however, we report large differences in their individual trajectories and note that a substantial number did not show a positive GMC development. Further research should examine GMC development with more possible influencing factors as well as over a longer time span to better understand differences in children’s GMC development. This may result in more individualized programs in PE lessons, enabling children to optimally develop their GMC, and better use of GMC assessment tools to monitor children’s development.
Evaluation of Placental Toxicity of Five Essential Oils and Their Potential Endocrine-Disrupting Effects
Authors: Fouyet Sophie , Olivier Elodie , Leproux Pascale , Dutot Mélody , Rat Patrice
Published: June 2022

Pregnant women may use EOs in case of morning sickness, nausea, stress management, etc. Little is known about the potential danger that EOs represent for the placenta and therefore for the pregnancy. Our aim was to explore and compare the placental toxicity and potential endocrine disrupting effects of niaouli, orange, tea tree, wintergreen and ylang-ylang EOs, and their key compounds: 4-terpineol, 1,8-cineol, limonene, methyl salicylate and benzyl salicylate. We studied the release of four hormones and the activation of P2X7 receptor in JEG-Tox human placental cells as key biomarkers for endocrine toxicity. We observed that niaouli, orange, tea tree, wintergreen and ylang-ylang EOs and their key components disrupted at least one of the studied hormones but none of them activated the P2X7 cell death receptor. The tested EOs appear then to be more hormonal modulators rather than EDCs in human placental cells. The hormonal effects observed with the key components were very different from those observed with the EOs. EOs are very complex mixtures, and it is essential to study whole EOs rather than their components individually in safety assessment.
Advanced Coating Materials for Machining Processes
Authors: Xu Jinyang , El Mansori Mohamed
Published: June 2022

Nowadays, the manufacturing community is facing considerable challenges in dealing with excessive wear and premature failures of cutting tools governing the machining processes [...]
Gloss of Varnished MDF Panels Veneered with Sanded and Thermally Compressed Veneer
Authors: Bekhta Pavlo , Lis Barbara , Krystofiak Tomasz , Tokarczyk Maciej , Bekhta Nataliya
Published: June 2022

The objective of this study was to investigate the gloss of different types of commercially manufactured varnish systems, including water-based (WB), polyurethane (PUR) and UV-cured (UV), applied on veneered MDF panels with sanded and thermally densified alder and birch wood veneers. The varnishes were applied at various numbers of layers on veneered panels. The gloss was measured at three angles of incident light: 20°, 60° and 85°. Statistical analysis showed that the type of varnish, the number of layers, the pre-treatment process, the wood species and direction of wood fibers significantly affect gloss of the coatings of veneered MDF panels. The type of varnish had a dominant effect on gloss. The highest gloss values were measured for the UV-varnished surface, and the lowest for WB- and PUR-varnished surfaces. Gloss was enhanced with an increase in the number of layers. Birch veneer provided higher gloss values compared to alder veneer. The gloss values measured along the wood fibers were higher than those measured across the fibers. No significant differences were found between the coatings created on sanded and thermally densified veneers for the average gloss values measured along the fibers at angles 60° and 85°. This study could have practical applications for producing value-added furniture elements using low-value wood species pre-treated by thermal compression.
The Effect of Various Polishing Systems on the Surface Roughness of Two Resin Composites—An In Vitro Study
Authors: Szczepaniak Marta Ewa , Krasowski Michał , Bołtacz-Rzepkowska Elżbieta
Published: June 2022

The long-term success of a composite restoration largely depends on its smoothness, which can be achieved by the appropriate polishing tools and material selection. The purpose of this study was to evaluate the surface roughness of two composite materials after the application of selected polishing systems. Filtek Ultimate (FU) and Filtek Z250 (FZ) were tested. Forty specimens of each material were prepared. After polymerization under a Mylar strip, the surface roughness of five samples from each group was measured. Subsequently, all specimens were ground by 600 grit sandpaper. The surface roughness of five samples per group was tested again. The samples from each group were randomly assigned to eight subgroups, and polished by Sof-Lex, Sof-Lex Diamond Polishing System, Super Snap, One Gloss, Astrobrush, Stainbuster, Enamel Shiny, and Jiffy Polishing System. The collected data were analyzed using the Shapiro–Wilk and Kruskal–Wallis tests. The lowest Ra coefficient after polishing is found in the Super Snap groups (FU—0.077 μm, FZ—0.085 μm). The lowest Rlr coefficient is measured in the Enamel Shiny group for FU (1.000), and for Sof-Lex, Sof-Lex Diamond Polishing System, and Jiffy Polishing Kit for FZ (1.001), and only slightly higher for Super Snap (FU—1.001, FZ—1.002). The roughest-measured surface is in the One Gloss group for FU (Ra—0.657 µm, Rlr—1.009), and Astrobrush group for FZ (Ra—0.525 μm, Rlr—0.011). Additionally, it was not confirmed that the nanoparticle material (FU) demonstrates better results than the microhybrid one (FZ). Different polishing systems produce varying surface roughness. The most effective polishing system is Super Snap. The structure of composites does not significantly affect their surface roughness after polishing.
DFT Study on Methanol Oxidation Reaction Catalyzed by PtmPdn Alloys
Authors: Yang Tingting , Xue Qian , Yu Xuewei , Qi Xueqiang , Wu Rui , Lu Shun , Gu Zhengrong , Jiang Jinxia , Nie Yao
Published: June 2022

Pt is widely used as the catalyst for methanol oxidation reaction (MOR) in direct methanol fuel cells (DMFC). However, the high cost and limited supply of pure Pt limit the commercialization of DMFC. Herein, MOR catalyzed by variously designed Pd-doped PtmPdn was studied with the density functional theory (DFT); the PtmPdn(111) surface was chosen since it is the most stable surface among various low-index surfaces. The hydrogens in methyl groups were priorly dehydrogenated on Pt(111), followed by hydrogen in the hydroxyl group. The effects of both the ratio of Pt:Pd and the type of the alloy on the activity of PtmPdn catalysts toward MOR were also studied; both ordered and disordered PtPd with the 1:1 ratio had better catalytic activity towards MOR than other catalysts. Specifically, the disordered Pt:Pdd with the Pt:Pd ratio of 1:1 had the best activity for the relatively stronger adsorption of COH, but the lowest binding with CO and a moderate d band center. The adsorptions of both COH and CO are key steps in the MOR, since the steps of CH3OH→CH2OH→CHOH→COH have downhill energy profiles, while COH→CO is an uphill reaction. In addition, the d band centers of the surface atoms move towards the Fermi level with the increase of the Pd content; the d band can also be tuned by changing the atom arrangement. These findings can be used as rules to design high-performance catalysts for MOR.
A Review of the Latest Developments in the Field of Additive Manufacturing Techniques for Nuclear Reactors
Authors: Li Hui , Fu Xiaolong , Zhang Liping , Zhang Yixiong , Jiang Lu , Pu Zhuo
Published: June 2022

This review paper provides insights the into current developments in additive manufacturing (AM) techniques. The comprehensive presentations about AM methods, material properties (i.e., irradiation damage, as-built defects, residual stresses and fatigue fracture), experiments, numerical simulations and standards are discussed as well as their advantages and shortages for the application in the field of nuclear reactor. Meanwhile, some recommendations that need to be focused on are presented to advance the development and application of AM techniques in nuclear reactors. The knowledge included in this paper can serve as a baseline to tailor the limitations, utilize the superiorities and promote the wide feasibilities of the AM techniques for wide application in the field of nuclear reactors.
Phylogeographic and Bioclimatic Determinants of the Dorsal Pattern Polymorphism in the Italian Wall Lizard, Podarcis siculus
Authors: Gallozzi Francesco , Colangelo Paolo , Senczuk Gabriele , Castiglia Riccardo
Published: June 2022

The geographic variability of the dorsal pattern (DP) of the Italian wall lizard, Podarcis siculus, across its native range was studied with the aim of understanding whether the distributions of this phenotypic trait were more shaped by allopatric differentiation rather than adaptive processes. A total of 1298 georeferenced observations scattered across the Italian peninsula and the main islands (Sicily, Corsica and Sardinia) were obtained from citizen science databases and five DPs were characterized by different shapes of the dark pattern (“reticulated”, “campestris”, “reticulated/campestris” and “striped”) or by absence of it (“concolor”). Frequencies of different DP phenotypes differ between the two main mtDNA lineages settled in central-northern and in southern Italy, respectively. This pattern may be indicative of a role of long-term allopatric historical processes in determining the observed pattern. The analysis also identified a putative wide area of secondary contact, in central southern Italy, characterized by high diversity of the DP. Generalized Linear Models (GLMs), used to estimate a possible association between bioclimatic variables and the observed phenotypic variation, showed that each of the five DPs is correlated to different environmental factors and show a different distribution of areas with high probability of occurrence. However, for all but one of the DPs, the area with the greatest probability does not correspond exactly to the real distribution of the DP. Conversely, the “concolor” phenotype does not seem related to any particular mtDNA lineage and it shows a preference for areas with high temperature and low rainfall. This is in agreement with the expectation of low amount of melanin of the dorsal pattern that, in the study areas, is characterized by a light uniform coloration which could confer a better thermoregulation ability in high temperatures environments to avoid overheating.
Phylogenetic Distance Metrics for Studies of Focal Species in Communities: Quantiles and Cumulative Curves
Authors: Gilbert Gregory S. , Parker Ingrid M.
Published: June 2022

The phylogenetic distance between species often predicts differences in ecologically important traits. The phylogenetic diversity and structure of biological communities can inform our understanding of the processes that shape those communities, and there is a well-developed framework for comparing phylogenetic structures of communities. However, particularly in studies of phylogenetic distances from one focal species to other members of its assemblage (a one-to-many framework), the standard metrics of community-wide studies encounter significant limitations due to the left-skewed distribution of pairwise phylogenetic distances in most biological communities. For studies that require estimating the degree of phylogenetic isolation of a focal taxon, the mean phylogenetic distance (MPD) usually provides little power to distinguish among taxa because it is heavily weighted by the many ways to be distantly related, whereas the nearest taxon distance (NTD) is highly idiosyncratic and ignores cases where multiple close relatives may contribute equally strongly to influence the focal species. Here we highlight the value of examining the cumulative distribution of phylogenetic distances in studies that take a focal-species approach. We describe and discuss the benefits of two new metrics. An integrated metric of phylogenetic distances (AUPhyDC) uses information from the whole cumulative distribution, whereas the tenth quantile (PD10) is an extremely simple metric that improves on NTD by capturing the influence of multiple close relatives on ecological interactions. Several recent examples found that PD10 did a better job of revealing ecological patterns than NTD or MPD. We provide R code to facilitate the use of these approaches and advocate for the inclusion of PD10 along with NTD and MPD in statistical packages for phylogenetic ecology.
Machine Learning and Clinical-Radiological Characteristics for the Classification of Prostate Cancer in PI-RADS 3 Lesions
Authors: Gravina Michela , Spirito Lorenzo , Celentano Giuseppe , Capece Marco , Creta Massimiliano , Califano Gianluigi , Collà Ruvolo Claudia , Morra Simone , Imbriaco Massimo , Di Bello Francesco , Sciuto Antonio , Cuocolo Renato , Napolitano Luigi , La Rocca Roberto , Mirone Vincenzo , Sansone Carlo , Longo Nicola
Published: June 2022

The Prostate Imaging Reporting and Data System (PI-RADS) classification is based on a scale of values from 1 to 5. The value is assigned according to the probability that a finding is a malignant tumor (prostate carcinoma) and is calculated by evaluating the signal behavior in morphological, diffusion, and post-contrastographic sequences. A PI-RADS score of 3 is recognized as the equivocal likelihood of clinically significant prostate cancer, making its diagnosis very challenging. While PI-RADS values of 4 and 5 make biopsy necessary, it is very hard to establish whether to perform a biopsy or not in patients with a PI-RADS score 3. In recent years, machine learning algorithms have been proposed for a wide range of applications in medical fields, thanks to their ability to extract hidden information and to learn from a set of data without previous specific programming. In this paper, we evaluate machine learning approaches in detecting prostate cancer in patients with PI-RADS score 3 lesions via considering clinical-radiological characteristics. A total of 109 patients were included in this study. We collected data on body mass index (BMI), location of suspicious PI-RADS 3 lesions, serum prostate-specific antigen (PSA) level, prostate volume, PSA density, and histopathology results. The implemented classifiers exploit a patient’s clinical and radiological information to generate a probability of malignancy that could help the physicians in diagnostic decisions, including the need for a biopsy.