bluuebunny/crossref_metadata_2025 huggingface dataset:
Winogradskyella exilis sp. nov., isolated from the starfish Stellaster equestris, and emended description of the genus Winogradskyella
Authors: Ivanova Elena P. , Christen Richard , Gorshkova Nataliya M. , Zhukova Natalia V. , Kurilenko Valeriya V. , Crawford Russell J. , Mikhailov Valery V.
Published: November 2009

A pale-yellowish-pigmented strain, 022-2-26T, was isolated from a starfish, Stellaster equestris. Cells of strain 022-2-26T were Gram-negative short rods that were chemo-organotrophic, alkalitolerant and mesophilic. The predominant menaquinone was MK-6. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1, C15 : 0, iso-C15 : 0 2-OH and iso-C17 : 0 3-OH (together representing 87 % of the total fatty acids). The DNA G+C content was 30.1 mol%. A 16S rRNA gene sequence of the isolate was determined and phylogenetic analyses revealed that strain 022-2-26T formed a robust clade (neighbour-joining algorithm with a bootstrap value of 95 % and parsimony and maximum-likelihood algorithms) with type strains of species in the genus Winogradskyella. The closest phylogenetic neighbour of strain 022-2-26T was Winogradskyella poriferorum UST030701-295T (96 % 16S rRNA gene sequence similarity; 59 differences between sequences). On the basis of the phenotypic and chemotaxonomic characteristics and the phylogenetic evidence, it is proposed that strain 022-2-26T represents a novel species, Winogradskyella exilis sp. nov. The type strain is 022-2-26T (=KMM 6013T =CIP 109976T).
Citricoccus parietis sp. nov., isolated from a mould-colonized wall and emended description of Citricoccus alkalitolerans Li et al. 2005
Authors: Schäfer Jenny , Martin Karin , Kämpfer Peter
Published: November 2009

A Gram-positive, coccoid-shaped organism (strain 02-Je-010T), forming yellow-pigmented colonies was isolated from the wall of an indoor environment. On the basis of 16S rRNA gene sequence similarity studies, it was shown that strain 02-Je-010Tbelongs to the genusCitricoccuswith sequence similarities of 98.9 % toCitricoccus alkalitoleransDSM 15665Tand 98.6 % toCitricoccus muralisDSM 14442T. Cell-wall sugars were mannose and glucose. The diagnostic diamino acid of the peptidoglycan was lysine. The major menaquinones detected were MK-9(H2) and MK-8(H2). The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol and moderate amounts of two unknown phospholipids and two unknown glycolipids. The fatty acid profile comprised major amounts of anteiso-C15 : 0, anteiso-C17 : 0and iso-C15 : 0. All these data supported the affiliation of strain 02-Je-010Tto the genusCitricoccus. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 02-Je-010Tfrom the two recognizedCitricoccusspecies. For these reasons, strain 02-Je-010Trepresents a novel species, for which the nameCitricoccus parietissp. nov. is proposed, with the type strain 02-Je-010T(=CCUG 57388T=CCM 7609T).
Epilithonimonas lactis sp. nov., isolated from raw cow'smilk
Authors: Shakéd Tamar , Hantsis-Zacharov Elionora , Halpern Malka
Published: November 2009

A Gram-staining-negative, rod-shaped, oxidase-positive, aerobic, non-motile and yellow-pigmented bacterial strain containing flexirubin type pigments, designated H1T, was isolated from raw cow'smilk in Israel.16S rRNA gene sequence analysis indicated that the isolate should be placed in the genus Epilithonimonas (family Flavobacteriaceae, phylum Bacteroidetes). The level of 16S rRNA gene sequence similarity between strain H1T and the type strain of Epilithonimonas tenax was 97.6 %. Strain H1T grew at 5–33 °C and with 0–2.0 % NaCl. The dominant cellular fatty acids of strain H1T were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1
ω7c), and the DNA G+C content was 38.0 mol%. On the basis of phenotypic properties and phylogenetic distinctiveness, the milk isolate is classified as a new species in the genus Epilithonimonas, for which the name Epilithonimonas lactis sp. nov. (type strain H1T =LMG 24401T =DSM 19921T) is proposed.
Wandonia haliotis gen. nov., sp. nov., a marine bacterium of the family Cryomorphaceae, phylum Bacteroidetes
Authors: Lee Dong-Heon , Choi Eun-Kyoung , Moon Sung-Ran , Ahn Samyoung , Lee Young Sun , Jung Jae Sung , Jeon Che Ok , Whang Kyung-Sook , Kahng Hyung-Yeel
Published: November 2009

A novel, strictly aerobic, Gram-stain-negative, yellow-orange-pigmented, rod-shaped bacterium was isolated from abalone (Haliotis discus) under aquaculture in seawater off the Wando coast, Southern Korea, and subjected to a polyphasic taxonomic study. Cells of strain Haldis-1T were catalase- and oxidase-positive rods with flexirubin pigments. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Haldis-1T formed a distinct lineage within the family Cryomorphaceae and could be distinguished from the related genera Lishizhenia and Fluviicola. Strain Haldis-1T shared 16S rRNA gene sequence similarities of 92.5 and 92.4 % with Lishizhenia caseinilytica UST040201-001T and Fluviicola taffensis RW262T, respectively. The DNA G+C content was 38.1 mol% and the major respiratory quinone was MK-7. The predominant cellular fatty acids were iso-C15 : 0 (38.6 %), C15 : 0 2-OH (20.3 %) and C15 : 0 (10.7 %). Growth was observed at 25–42 °C (optimum 30–37 °C) and at pH 6.5–9.5 (optimum pH 6.5–8.0). On the basis of polyphasic analysis of phenotypic, genotypic and phylogenetic data, strain Haldis-1T represents a novel genus and species within the family Cryomorphaceae in the phylum Bacteroidetes, for which the name Wandonia haliotis gen. nov., sp. nov. is proposed. The type strain is Haldis-1T (=KCTC 22610T =NBRC 105642T).
Candida olivae sp. nov., a novel yeast species from ‘Greek-style’ black olive fermentation
Authors: Nisiotou Aspasia A. , Panagou Efstathios Z. , Nychas George-John E.
Published: November 2009

Two yeast strains (FMCC Y-1T and FMCC Y-2) were recovered during a survey of the yeast biota associated with fermenting black olives, collected from an olive tree (Olea europaea L. cv. ‘Conservolea’) orchard in Central Greece. Phylogenetic analysis based on rRNA gene sequences (18S, 26S, and 5.8S–ITS) indicated that the two strains represent a separate species within the Candida membranifaciens clade, in close relation to Candida blattariae NRRL Y-27703T. Electrophoretic karyotyping and physiological analysis support the affiliation of the two strains to a novel species as Candida olivae sp. nov. The novel strains are conspecific with two Candida sp. strains previously isolated from the Mid-Atlantic Ridge hydrothermal fields [Gadanho & Sampaio (2005). Microb Ecol
50, 408–417], indicating that Candida olivae sp. nov. may occupy diverse ecological niches. FMCC Y-1T (=CBS 11171T =ATCC MYA-4568T) is the type strain.
Alkalibacillus flavidus sp. nov., isolated from a marine solar saltern
Authors: Yoon Jung-Hoon , Kang So-Jung , Jung Yong-Taek , Lee Mi-Hwa , Oh Tae-Kwang
Published: November 2009

A Gram-stain-positive, motile, rod-shaped bacterial strain, ISL-17T, was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain ISL-17T grew optimally at pH 8.5–9.0, at 37 °C and in the presence of approximately 10 % (w/v) NaCl. It contained meso-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan, MK-7 as the predominant menaquinone and iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 as the major fatty acids. The DNA G+C content was 48.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-17T fell within the genus Alkalibacillus, clustering with Alkalibacillus
salilacus BH163T with a bootstrap resampling value of 100 %. Strain ISL-17T exhibited 98.2 % 16S rRNA gene sequence similarity to A. salilacus BH163T and 95.8–96.5 % similarity to the type strains of the other Alkalibacillus species. The mean DNA–DNA relatedness value between strain ISL-17T and A. salilacus KCTC 3916T was 19 %. The phenotypic properties of strain ISL-17T, together with its phylogenetic and genetic distinctiveness, enable this strain to be differentiated from recognized Alkalibacillus species. On the basis of phenotypic, phylogenetic and genetic data, strain ISL-17T represents a novel species within the genus Alkalibacillus, for which the name Alkalibacillus flavidus sp. nov. is proposed; the type strain is ISL-17T (=KCTC 13258T =CCUG 56753T).
Haladaptatus cibarius sp. nov., an extremely halophilic archaeon from seafood, and emended description of the genus Haladaptatus
Authors: Roh Seong Woon , Lee Myung-Lip , Bae Jin-Woo
Published: November 2009

A novel, extremely halophilic archaeon, D43T, was isolated from traditional salt-fermented seafood in Korea. The cells were Gram-negative-staining and motile. The strain grew at 15–50 °C, 10–30 % (w/v) NaCl and pH 6.0–8.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain D43Tis affiliated with the familyHalobacteriaceaein the domainArchaeaand had 95.5 % 16S rRNA gene sequence similarity withHaladaptatus paucihalophilusDX253T. The sequence from strain D43Tformed a clade with those fromHap. paucihalophilusregardless of which tree-generating algorithm was used. DNA–DNA hybridization experiments showed 25.8 % relatedness between the isolate andHap. paucihalophilusKCTC 4006T. Major lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two unidentified glycolipids. The DNA G+C content of the isolate was 56.5 mol%. On the basis of this polyphasic taxonomic study, strain D43Trepresents a novel species in the genusHaladaptatus, for which the nameHaladaptatus cibariussp. nov. is proposed. The type strain is D43T(=DSM 19505T=JCM 15962T).
Clostridium hydrogeniformans sp. nov. and Clostridium cavendishii sp. nov., hydrogen-producing bacteria from chlorinated solvent-contaminated groundwater
Authors: Bowman Kimberly S. , Dupré Rachael E. , Rainey Fred A. , Moe William M.
Published: November 2009

Four hydrogen-producing, aerotolerant, anaerobic bacterial strains isolated from chlorinated solvent-contaminated groundwater were characterized using a polyphasic approach. Three of the strains, designated BL-18, BL-19 and BL-20T, were found to be identical in 16S rRNA gene sequences and in phenotypic properties. Cells of these strains are Gram-positive-staining, spore-forming, motile rods with peritrichous flagella. Growth occurred at 15–40 °C, pH 5.0–10.0 and at NaCl concentrations up to 5 % (w/v). Acid was produced in fermentation of cellobiose, fructose, galactose (weak), glucose, maltose and salicin. Products of fermentation in PYG medium were acetate, butyrate, ethanol, formate, carbon dioxide and hydrogen. Dominant cellular fatty acids when grown in PYG medium were C13 : 0 iso, C16 : 0, C13 : 0 anteiso, C15 : 0 iso and C15 : 0 anteiso. The genomic DNA G+C content was 30.4 mol%. These isolates can be differentiated from their closest phylogenetic relative, the cluster I Clostridium species Clostridium frigidicarnis (97.2 % similar to the type strain in 16S rRNA gene sequence), on the basis of phenotypic and chemotaxonomic properties. The other strain characterized in this study, BL-28T, was Gram-positive-staining with spore-forming, rod-shaped cells. Growth occurred at 15–46 °C, pH 6.0–8.5 and at NaCl concentrations up to 3 % (w/v). Acid was produced from cellobiose, dextran, fructose (weak), glucose, maltose, salicin and trehalose. End products of PYG fermentation included acetate, butyrate, pyruvate, carbon dioxide and hydrogen. Dominant cellular fatty acids from cells grown in PYG medium at 30 °C were C14 : 0, C14 : 0 dimethyl aldehyde, C16 : 0 and C12 : 0. The DNA G+C content was 28.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BL-28T falls within cluster I of the genus Clostridium, but with ≤95.2 % identity with previously described species. On the basis of results presented here, strains BL-20T (=NRRL B-51348T =DSM 21757T) and BL-28T (=NRRL B-51352T =DSM 21758T) are proposed as the type strains of novel species of the genus Clostridium with the names Clostridium hydrogeniformans sp. nov. and Clostridium cavendishii sp. nov., respectively.
Rhodococcus jialingiae sp. nov., an actinobacterium isolated from sludge of a carbendazim wastewater treatment facility
Authors: Wang Zhichun , Xu Jingliang , Li Yan , Wang Kun , Wang Yangyang , Hong Qing , Li Wen-Jun , Li Shun-Peng
Published: November 2009

A novel carbendazim-degrading actinobacterium, designated djl-6-2T, was isolated from the sludge of a carbendazim wastewater treatment facility in Jiangsu province, China. The morphological and chemotaxonomic properties of the isolate were typical of members of the genusRhodococcus. Strain djl-6-2Tformed a coherent cluster withRhodococcus qingshengiidjl-6T,Rhodococcus baikonurensisDSM 44587T,Rhodococcus erythropolisDSM 43066TandRhodococcus globerulusDSM 43954Tin 16S rRNA gene sequence analysis. The results of DNA–DNA hybridization with the above strains (27.7, 19.3, 18.6 and 10.6 % relatedness, respectively), in combination with differences in biochemical and physiological properties, suggest that strain djl-6-2Tshould be classified within a novel species of the genusRhodococcus, for which the nameRhodococcus jialingiaesp. nov. is proposed, with djl-6-2T(=DSM 45257T=CCTCC AB 208292T) as the type strain.
Micromonospora tulbaghiae sp. nov., isolated from the leaves of wild garlic, Tulbaghia violacea
Authors: Kirby Bronwyn M. , Meyers Paul R.
Published: November 2009

A novel actinomycete, strain TVU1T, was isolated from leaves of the indigenous South African plant Tulbaghia violacea. Applying a polyphasic approach, the isolate was identified as a member of the genus Micromonospora. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain TVU1T was most closely related to Micromonospora echinospora DSM 43816T. However, phylogenetic analysis based on gyrB gene sequences showed that strain TVU1T was most closely related to the type strains of Micromonospora aurantiaca and Micromonospora chalcea. DNA–DNA relatedness values between strain TVU1T and the type strains of M. echinospora, M. aurantiaca and M. chalcea were 7.6±4.5, 45.9±2.0 and 60.9±4.5 %, respectively. Strain TVU1T could be distinguished from the type strains of all three of these species by several physiological characteristics, such as colony colour, NaCl tolerance, growth temperature range and sole carbon source utilization pattern. Strain TVU1T (=DSM 45142T=NRRL B-24576T) therefore represents a novel species for which the name Micromonospora tulbaghiae sp. nov. is proposed.
Geomicrobium halophilum gen. nov., sp. nov., a moderately halophilic and alkaliphilic bacterium isolated from soil
Authors: Echigo Akinobu , Minegishi Hiroaki , Mizuki Toru , Kamekura Masahiro , Usami Ron
Published: November 2009

Two moderately halophilic and alkaliphilic bacteria, designated strains BH1Tand HN5, were isolated from forest soil and garden soil, respectively, in Japan. Cells of strains BH1Tand HN5 were non-motile, aerobic, bean-shaped, formed irregular clusters with 2–20 cells, Gram-positive and contained A1γ,meso-diaminopimelic acid-type murein. Spore formation was not detected. Growth occurred in 5–25 % (w/v) NaCl (optimum, 10–15 %, w/v), at pH 6.0–10.0 (optimum, pH 8.5–9.0) and at 20–40 °C (optimum, 30 °C). The predominant isoprenoid quinones were menaquinone-7 and menaquinone-6. The phospholipids were diphosphatidylglycerol and phosphatidylglycerol. The major cellular fatty acids were i-C15 : 0, i-C17 : 0and i-C18 : 0. The DNA G+C content of strains BH1Tand HN5 was 45 and 46 mol%, respectively. The 16S rRNA gene sequences of the two strains were 99.9 % similar. DNA–DNA hybridization results indicated high levels of relatedness (88 and 85 % reciprocally). Similarities with recognized species were less than 90.2 %. The phylogenetic and phenotypic characteristics indicated that strains BH1Tand HN5 represent a novel species in a new genus, for which the nameGeomicrobium halophilumgen. nov., sp. nov. is proposed. The type strain is BH1T(=JCM 15647T=DSM 21769T).
Marichromatium fluminis sp. nov., a slightly alkaliphilic, phototrophic gammaproteobacterium isolated from river sediment
Authors: Sucharita K. , Shiva Kumar E. , Sasikala Ch. , Panda B. B. , Takaichi S. , Ramana Ch. V.
Published: November 2009

An anoxygenic, phototrophic gammaproteobacterium designated strain JA418T was isolated from a sediment sample collected from the Baitarani River, Orissa, India. The bacterium was a Gram-negative, motile rod with a single polar flagellum. Bacteriochlorophyll a and rhodopin were the major photosynthetic pigments. The organism grew best at slightly alkaline pH (8–8.5) and lacked chemotrophic growth. The major fatty acids were C16 : 0, C16 : 1
ω7c/C16 : 1
ω6c and C18 : 1
ω7c. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strain JA418T clusters with species of the genus Marichromatium belonging to the class Gammaproteobacteria. The highest 16S rRNA gene sequence similarities of strain JA418T were found with the type strains of Marichromatium gracile (95.9 %), Marichromatium indicum (95.6 %), Marichromatium purpuratum (95.5 %) and Marichromatium bheemlicum (95.6 %). The DNA base composition of strain JA418T was 71.4 mol% G+C (by HPLC). Based on the 16S rRNA gene sequence analysis and physiological and chemotaxonomic characteristics, strain JA418T is sufficiently different from other Marichromatium species to merit the description of a novel species, Marichromatium fluminis sp. nov., to accommodate it. The type strain is JA418T (=KCTC 5717T =NBRC 105221T).
Serratia glossinae sp. nov., isolated from the midgut of the tsetse fly Glossina palpalis gambiensis
Authors: Geiger A. , Fardeau M.-L. , Falsen E. , Ollivier B. , Cuny G.
Published: November 2009

We report the isolation of a novel bacterium, strain C1T, from the midgut of the tsetse fly Glossina palpalis gambiensis, one of the vector insects responsible for transmission of the trypanosomes that cause sleeping sickness in sub-Saharan African countries. Strain C1T is a motile, facultatively anaerobic, rod-like bacterium (0.8–1.0 μm in diameter; 2–6 μm long) that grows as single cells or in chains. Optimum growth occurred at 25–35 °C, at pH 6.7–8.4 and in medium containing 5–20 g NaCl l−1. The bacterium hydrolysed urea and used l-lysine, l-ornithine, citrate, pyruvate, d-glucose, d-mannitol, inositol, d-sorbitol, melibiose, amygdalin, l-arabinose, arbutin, aesculin, d-fructose, d-galactose, glycerol, maltose, d-mannose, raffinose, trehalose and d-xylose; it produced acetoin, reduced nitrate to nitrite and was positive for β-galactosidase and catalase. The DNA G+C content was 53.6 mol%. It was related phylogenetically to members of the genus Serratia, family Enterobacteriaceae, the type strain of Serratia fonticola being its closest relative (99 % similarity between 16S rRNA gene sequences). However, DNA–DNA relatedness between strain C1T and S. fonticola DSM 4576T was only 37.15 %. Therefore, on the basis of morphological, nutritional, physiological and fatty acid analysis and genetic criteria, strain C1T is proposed to be assigned to a novel Serratia species, Serratia glossinae sp. nov. (type strain C1T =DSM 22080T =CCUG 57457T).
Ruegeria pelagia is a later heterotypic synonym of Ruegeria mobilis
Authors: Lai Qiliang , Yuan Jun , Li Fuying , Zheng Tianling , Shao Zongze
Published: November 2009

The 16S rRNA genes of Ruegeria pelagia NBRC 102038T and Ruegeria mobilis NBRC 101030T were resequenced and the results confirmed that they differ by only one base in their almost full-length sequences (1425 nt). The gyrB gene sequence similarity between the two strains was also high (97.7 %). The outcome of API 20NE, API ZYM and antibiotic susceptibility tests showed that the two strains show only one difference, in β-galactosidase activity, in API tests and five differences in susceptibility among 30 tested antibiotics. In addition, similar BOX-PCR fingerprints were obtained and the DNA–DNA relatedness between the two strains was 91±4 %. On the basis of these results, it is concluded that Ruegeria pelagia Lee et al. 2007 is a later heterotypic synonym of Ruegeria mobilis Muramatsu et al. 2007.
Granulosicoccus coccoides sp. nov., isolated from leaves of seagrass (Zostera marina)
Authors: Kurilenko Valerie V. , Christen Richard , Zhukova Natalia V. , Kalinovskaya Nataliya I. , Mikhailov Valery V. , Crawford Russell J. , Ivanova Elena P.
Published: November 2009

A non-pigmented, motile, Gram-negative bacterium, strain Z 271T, was isolated from the surface of leaves of the seagrassZostera marinawhich was collected in Troitza Bay (Sea of Japan, Pacific Ocean). The new isolate grew between 5 °C and 28 °C and was slightly halophilic, tolerating environments containing up to 5 % (w/v) NaCl. Strain Z 271Twas able to degrade Tweens 20, 40 and 80 and partially degrade gelatin, but was unable to degrade casein. Phosphatidylethanolamine (36.9 %) and phosphatidylglycerol (63.1 %) were the predominant phospholipids. The major fatty acids included C18 : 1ω7c(43.7 %), C16 : 1ω7c(31.1 %) and C16 : 0(16.8 %). The main respiratory quinone was Q-8. The DNA–DNA relatedness value of strain Z 271TwithGranulosicoccus antarcticusIMCC3135Twas 35 %. The G+C content of the DNA of strain Z 271Twas 60.2 mol%. On the basis of phenotypic and genotypic characteristics and 16S rRNA gene sequence analysis, strain Z 271Trepresents a novel species of the genusGranulosicoccusfor which the nameGranulosicoccus coccoidessp. nov. is proposed. The type strain is Z 271T(=KMM 6014T=CIP 109923T).
Gallaecimonas pentaromativorans gen. nov., sp. nov., a bacterium carrying 16S rRNA gene heterogeneity and able to degrade high-molecular-mass polycyclic aromatic hydrocarbons
Authors: Rodríguez-Blanco Arturo , Vetion Gilles , Escande Marie-Line , Delille Daniel , Ghiglione Jean-François
Published: November 2009

A Gram-negative, rod-shaped, halotolerant bacterium, designated strain CEE_131T, which degraded high-molecular-mass polycyclic aromatic hydrocarbons of four and five rings, was isolated from intertidal sediment of Corcubion Ria in Cee, A Coruña, Spain. Direct sequencing showed ambiguities and suggested heterogeneity. Cloned 16S rRNA gene sequence PCR products yielded five different sequences varying at five positions. Strain CEE_131Tshowed rather distant relationships to its phylogenetically closest neighbours, including the generaRheinheimeraandSerratia, exhibiting 91 % sequence similarity withRheinheimera perlucidaBA131TandSerratia proteamaculanssubsp.quinovoraDSM 4597T. The major fatty acids were C16 : 1ω7c, C16 : 0and C18 : 1ω7c. The DNA G+C content was 41.7 mol%. On the basis of these distinct phenotypic and genotypic characteristics, strain CEE_131Tis considered to represent a novel species in a new genus in the classGammaproteobacteria, for which the nameGallaecimonas pentaromativoransgen. nov., sp. nov. is proposed. The type strain is CEE_131T(=DSM 21945T=CECT 7479T).
Brevundimonas basaltis sp. nov., isolated from black sand
Authors: Choi Jung-Hye , Kim Min-Soo , Roh Seong Woon , Bae Jin-Woo
Published: November 2009

A Gram-negative, aerobic, rod-shaped, motile Brevundimonas-like bacterial strain, J22T, was isolated from black sand collected from Soesoggak, Jeju Island, Korea. Growth of strain J22T was observed in R2A medium at temperatures between 10 and 42 °C (optimum 30 °C), between pH 6.5 and 10.5 (optimum pH 7.5) and at a NaCl concentration between 0 and 4 % (w/v) (optimum 0–1 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J22T belonged to the genus Brevundimonas, with high sequence similarities of >97 % to the sequence of the type strains Brevundimonas
alba CB88T, Brevundimonas
lenta DS-18T, Brevundimonas
variabilis CB17T, Mycoplana bullata TK0051T, Brevundimonas
kwangchunensis KSL-102T, Brevundimonas
intermedia CB63T, Brevundimonas
subvibrioides CB81T and Brevundimonas
bacteroides CB7T. Strain J22T exhibited DNA–DNA relatedness values of less than 22.2 % with the phylogenetically related species of the genus Brevundimonas. The DNA G+C content of strain J22T was 66.3 mol%. The predominant cellular fatty acids were C18 : 1
ω7c, C16 : 0 and C16 : 1
ω9c; C12 : 0 3-OH was present, which chemotaxonomically characterizes the members of the genus Brevundimonas. Phylogenetic, genomic and biochemical characteristics served to differentiate this isolate from recognized members of the genus Brevundimonas. Strain J22T (=KCTC 22177T=JCM 15911T) should be classified as a novel species in the genus Brevundimonas, for which the name Brevundimonas basaltis sp. nov. is proposed.
Yimella lutea gen. nov., sp. nov., a novel actinobacterium of the family Dermacoccaceae
Authors: Tang Shu-Kun , Wu Jin-Yuan , Wang Yun , Schumann Peter , Li Wen-Jun
Published: November 2009

A Gram-stain-positive, coccoid, non-motile, halotolerant actinobacterium, designated YIM 45900T, was found as a contaminant on an agar plate in the laboratory of Yunnan Institute of Microbiology, China. The peptidoglycan type was A4α with an l-Lys–l-Ser–d-Asp interpeptide bridge. The cell-wall sugars contained galactose and fucose. The predominant menaquinone was MK-8(H4). The major fatty acids were iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The polar lipids contained diphosphatidylglycerol, phosphatidylinositol, a glucosamine-containing phospholipid and an unknown phospholipid. The DNA G+C content was 65.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism falls within the radius of the suborder Micrococcineae and its closest phylogenetic neighbours are the genera of the family Dermacoccaceae. Strain YIM 45900T showed 16S rRNA gene sequences similarity values of 93.1–95.9 % with members of the genera Dermacoccus, Demetria and Kytococcus. On the basis of the phylogenetic and phenotypic characteristics of the actinobacterium, a novel genus and species, Yimella lutea gen. nov., sp. nov., are proposed. The type strain of Yimella lutea is YIM 45900T (=DSM 19828T =KCTC 19231T =CCTCC AB 207007T).
Microbacterium agarici sp. nov., Microbacterium humi sp. nov. and Microbacterium pseudoresistens sp. nov., isolated from the base of the mushroom Agaricus blazei
Authors: Young C.-C. , Busse H.-J. , Langer S. , Chu Jiunn-Nan , Schumann P. , Arun A. B. , Shen Fo-Ting , Rekha P. D. , Kämpfer P.
Published: November 2009

Three Gram-positive, rod-shaped bacteria (strains CC-SBCK-209T, CC-12309Tand CC-5209T) were isolated from the stalk of the edible mushroomAgaricus blazeigrown in the laboratory. 16S rRNA gene sequence analysis indicated that all three isolates clearly belonged to the genusMicrobacterium. Strains CC-SBCK-209Tand CC-12309Twere most related closely to the type strain ofMicrobacterium halotolerans(95.9 and 96.1 %16S rRNA gene sequence similarity, respectively). These two novel strains shared 97.9 % 16S rRNA gene sequence similarity. Levels of similarity to the type strains of all other recognizedMicrobacteriumspecies were lower than 95.5 %. The third strain (CC-5209T) showed the highest 16S rRNA gene sequence similarity to the type strain ofMicrobacterium resistens(97.6 %); levels of similarity to the type strains of all other recognizedMicrobacteriumspecies were lower than 96 %. The quinone systems of strains CC-SBCK-209T, CC-12309Tand CC-5209Tconsisted of MK-11/MK-12, MK-11/MK-10 and MK-13 as major compounds, respectively. All three strains contained ornithine in their peptidoglycan. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. The polyamine pattern consisted of spermidine and spermine as predominant components. Fatty acid profiles (anteiso-C15 : 0, iso-C16 : 0and anteiso-C17 : 0as major components) supported the affiliation of all three strains to the genusMicrobacterium. The results of physiological and biochemical tests and DNA–DNA hybridization experiments allowed the clear phenotypic and genotypic differentiation of strains CC-SBCK-209Tand CC-12309TfromM. halotoleransand other closely relatedMicrobacteriumspecies. Strain CC-5209Tcould be differentiated clearly fromM. resistensboth genotypically and phenotypically. Based on these data, the novel strains are considered to represent three novel species of the genusMicrobacterium. The names proposed for these organisms areMicrobacterium agaricisp. nov. [type strain CC-SBCK-209T(=DSM 21798T=CCM 7686T)],Microbacterium humisp. nov. [type strain CC-12309T(=DSM 21799T=CCM 7687T)] andMicrobacterium pseudoresistenssp. nov. [type strain CC-5209T(=DSM 22185T=CCM 7688T)].
Bacillus halochares sp. nov., a halophilic bacterium isolated from a solar saltern
Authors: Pappa A. , Sánchez-Porro C. , Lazoura P. , Kallimanis A. , Perisynakis A. , Ventosa A. , Drainas C. , Koukkou A. I.
Published: November 2009

A novel halophilic bacterium, designated strain MSS4T, was isolated from the solar salterns of Mesolongi, Greece. The micro-organism, a motile, Gram-stain-positive, aerobic rod, proliferated at salinities of 1.0–4.0 M NaCl, with optimal growth at 2.5 M NaCl. Endospores were not observed. Strain MSS4T showed optimal growth at 37 °C and pH 8.0. The G+C content of its DNA was 47.2 mol%. The polar lipid pattern of strain MSS4T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidic acid and phosphatidylethanolamine. It possessed anteiso-C15 : 0, C18 : 0, C16 : 0 and anteiso-C17 : 0 as the major fatty acids (altogether representing 84.7 % of the total). The predominant isoprenoid quinone was MK-7. The cell-wall peptidoglycan contained meso-diaminopimelic acid. 16S rRNA gene sequence analysis showed that the new isolate has 96.1 % similarity to Bacillus qingdaonensis CM1T and Bacillus aidingensis 17-5T, 95.5 % to Bacillus salarius BH169T and lower similarity to other Bacillus species. These results justify the assignment of strain MSS4T to a novel species within the genus Bacillus, for which the name Bacillus halochares sp. nov. is proposed. The type strain is MSS4T (=LMG 24571T =DSM 21373T).
Alistipes indistinctus sp. nov. and Odoribacter laneus sp. nov., common members of the human intestinal microbiota isolated from faeces
Authors: Nagai Fumiko , Morotomi Masami , Watanabe Yohei , Sakon Hiroshi , Tanaka Ryuichiro
Published: November 2009

Two anaerobic, non-spore-forming, non-motile, Gram-negative-staining bacteria, strains YIT 12060T and YIT 12061T, were isolated from human faeces. Cells of strain YIT 12060T were coccoid to rod-shaped with round ends, positive for catalase, negative for indole and oxidase production, produced succinic and acetic acids as end products of glucose metabolism in peptone/yeast extract/glucose medium and had a DNA G+C content of 55.2 mol%. The main respiratory quinones were MK-10 (40 %) and MK-11 (57 %). Fatty acid analysis demonstrated the presence of a high concentration of iso-C15 : 0 (56 %). Following 16S rRNA gene sequence analysis, this strain was found to be most closely related to species of the genus Alistipes, with 90.9–92.6 % gene sequence similarities to type strains of this species. Phylogenetic analysis and biochemical data supported the affiliation of strain YIT 12060T to the genus Alistipes of the family ‘Rikenellaceae’. Strain YIT 12060T therefore represents a novel species of the genus Alistipes for which the name Alistipes indistinctus sp. nov. is proposed; the type strain is YIT 12060T (=DSM 22520T=JCM 16068T). Cells of the other isolate, strain YIT 12061T, were pleomorphic rods that were asaccharolytic, catalase- and oxidase-negative, positive for gelatin hydrolysis and indole production, produced small amounts of succinic, acetic and iso-valeric acids as end products of metabolism in peptone/yeast extract medium and had a DNA G+C content of approximately 42.4 mol%. On the basis of 16S rRNA gene sequence similarity values, this strain was shown to belong to the family ‘Porphyromonadaceae’ and related to the type strains of Odoribacter splanchnicus (89.6 %) and Odoribacter denticanis (86.2 %); similarity values with strains of recognized species within the family ‘Porphyromonadaceae’ were less than 84 %. Biochemical data supported the affiliation of strain YIT 12061T to the genus Odoribacter. Strain YIT 12061T therefore represents a novel species for which the name Odoribacter laneus sp. nov. is proposed; the type strain is YIT 12061T (=DSM 22474T=JCM 16069T).
Aeromonas taiwanensis sp. nov. and Aeromonas sanarellii sp. nov., clinical species from Taiwan
Authors: Alperi Anabel , Martínez-Murcia Antonio J. , Ko Wen-Chien , Monera Arturo , Saavedra Maria J. , Figueras Maria J.
Published: November 2009

Two clinicalAeromonasstrains (A2-50Tand A2-67T) recovered from the wounds of two patients in Taiwan could not be assigned to any known species of this genus based on their 16S rRNA gene sequences, which showed similarities of 99.6–99.8 % to those of the type strains ofAeromonas caviae,A. trotaandA. aquariorum. TherpoDphylogenetic tree allocated these strains to two novel and independent phylogenetic lines, the neighbouring species beingA. caviae, the type strain of which showed 93.2 % similarity (56 bp differences) to strain A2-50Tand 92.2 % (63 bp differences) to strain A2-67T. A multilocus phylogenetic analysis of five housekeeping genes (gyrB,rpoD,recA,dnaJandgyrA; 3684 bp) confirmed that the two strains formed independent phylogenetic lineages within the genus. These data, together with phenotypic characterization and DNA–DNA reassociation results, revealed that these strains represent novelAeromonasspecies, for which the namesAeromonas taiwanensissp. nov. (type strain A2-50T=CECT 7403T=LMG 24683T) andAeromonas sanarelliisp. nov. (type strain A2-67T=CECT 7402T=LMG 24682T) are proposed.
Stenotrophomonas ginsengisoli sp. nov., isolated from a ginseng field
Authors: Kim Ho-Bin , Srinivasan Sathiyaraj , Sathiyaraj Gayathri , Quan Lin-Hu , Kim Se-Hwa , Bui Thi Phuong Nam , Liang Zhi-qi , Kim Yeon-Ju , Yang Deok-Chun
Published: November 2009

A Gram-negative, non-spore-forming, rod-shaped bacterium, designated strain DCY01T, was isolated from soil from a ginseng field in South Korea and was characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain DCY01T belonged to the Gammaproteobacteria and was most closely related to Stenotrophomonas koreensis KCTC 12211T (98.4 % similarity), Stenotrophomonas humi R-32729T (97.2 %), Stenotrophomonas terrae R-32768 (97.1 %), Stenotrophomonas maltophilia DSM 50170T (96.9 %) and Stenotrophomonas nitritireducens DSM 12575T (96.8 %). Chemotaxonomic analyses revealed that strain DCY01T possessed a quinone system with Q-8 as the predominant compound, and iso-C15 : 0 (28.2 %), C16 : 0 10-methyl (13.2 %), iso-C15 : 1 F (10.8 %) and C15 : 0 (7.5 %) as major fatty acids, corroborating assignment of strain DCY01T to the genus Stenotrophomonas. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The results of DNA–DNA hybridization and physiological and biochemical tests clearly demonstrated that strain DCY01T represents a species distinct from recognized Stenotrophomonas species. Based on these data, DCY01T (=KCTC 12539T=NBRC 101154T) should be classified as the type strain of a novel species of the genus Stenotrophomonas, for which the name Stenotrophomonas ginsengisoli sp. nov. is proposed.
Desulfosalsimonas propionicica gen. nov., sp. nov., a halophilic, sulfate-reducing member of the family Desulfobacteraceae isolated from a salt-lake sediment
Authors: Kjeldsen Kasper Urup , Jakobsen Trine Fredlund , Glastrup Jens , Ingvorsen Kjeld
Published: November 2009

A novel halophilic Gram-negative sulfate-reducing bacterium affiliated with the deltaproteobacterial family Desulfobacteraceae, strain PropAT, was isolated from the extreme hypersaline sediment of the northern arm of Great Salt Lake, Utah, USA. Comparative 16S rRNA gene sequence analysis showed that strain PropAT is the first cultured representative of a clade of phylotypes that have been retrieved from a range of geographically and ecologically distinct hypersaline environments. Strain PropAT shared ≤90 % 16S rRNA gene sequence identity with cultured strains within the family Desulfobacteraceae. Cells of strain PropAT were rod-shaped and sometimes motile. The strain required NaCl for growth and grew at salinities up to 200 g NaCl l−1 (optimum 60 g l−1). Growth was observed at 15–40 °C, optimum growth occurred at about 40 °C, while growth was absent at 10 and 45 °C. The pH range for growth was pH 6.0–8.3. Yeast extract (0.1 g l−1) was required for growth. C2–4 alcohols, C3–4 carboxylic acids, yeast extract and H2/acetate supported growth with sulfate as electron acceptor. Sulfate, thiosulfate and sulfite served as electron acceptors, but not elemental sulfur, nitrate or fumarate. The DNA G+C content of strain PropAT was 54.1 mol%. Based on the genotypic and physiological properties, we propose that strain PropAT represents a novel species within a novel genus, Desulfosalsimonas propionicica gen. nov., sp. nov. The type strain of Desulfosalsimonas propionicica is PropAT (=DSM 17721T =VKM B-2385T).
Georgenia soli sp. nov., isolated from iron-ore-contaminated soil in India
Authors: Kämpfer P. , Arun A. B. , Busse H.-J. , Langer S. , Young C.-C. , Chen W.-M. , Schumann P. , Syed A. A. , Rekha P. D.
Published: November 2009

A Gram-positive actinobacterium (CC-NMPT-T3T) was isolated from iron-ore-contaminated soil near New Mangalore Port, Karnataka, India. Based on 16S rRNA gene sequence similarity studies, strain CC-NMPT-T3Tbelongs to the genusGeorgeniaand is most closely related toGeorgenia muralis(98.6 %),Georgenia ruanii(97.4 %) andGeorgenia thermotolerans(97.4 %). The peptidoglycan is of the type A4αl-Lys←l-Glu. The predominant isoprenoid quinone is menaquinone MK-8(H4) and the polar lipid profile is composed of the predominant compound diphosphatidylglycerol, moderate amounts of a phosphatidylinositol-mannoside, phosphatidylinositol and minor amounts of another phosphatidylmannoside and phosphatidylglycerol. The major fatty acids of strain CC-NMPT-T3Tare anteiso-C15 : 0and iso-C15 : 0. The results of DNA–DNA hybridizations, and physiological and biochemical tests allowed a clear phenotypic differentiation of strain CC-NMPT-T3Tfrom all otherGeorgeniaspecies. Strain CC-NMPT-T3Trepresents a novel species, for which the nameGeorgenia solisp. nov. is proposed. The type strain is CC-NMPT-T3T(=DSM 21838T=CCM 7658T).
Labrys wisconsinensis sp. nov., a budding bacterium isolated from Lake Michigan water, and emended description of the genus Labrys
Authors: Albert Richard A. , Waas Nancy E. , Langer Stefan , Pavlons Shawn C. , Feldner Jamie L. , Rosselló-Mora Ramon , Busse Hans-Jürgen
Published: November 2009

Two facultatively anaerobic, budding bacterial strains, designated W1215-PCA4T and SRNK-1, were isolated from water from Lake Michigan, USA. The two strains showed identical ERIC-PCR-generated genomic fingerprints and shared 99.9 % 16S rRNA gene sequence similarity. Strain W1215-PCA4T showed highest 16S rRNA gene sequence similarities to Labrys monachus VKM B-1479T (95.8 %), Labrys methylaminiphilus DSM 16812T (95.1 %), Labrys okinawensis MAFF 210191T (96.0 %), Labrys miyagiensis G24103T (95.4 %), Labrys neptuniae BCRC 17578T (95.7 %) and Labrys portucalensis DSM 17916T (95.8 %). Data suggested that the two strains were members of a single novel species of the genus Labrys. The major cellular fatty acids of the two isolates were C18 : 1
ω7c, C19 : 0 cyclo ω8c and C16 : 0. Their polar lipid profiles were highly similar to that of Labrys monachus DSM 5896T. The primary quinone was ubiquinone Q-10, with minor amounts of Q-9 and Q-11. sym-Homospermidine was the predominant polyamine, with putrescine present in moderate amounts. The two strains were identical in terms of their biochemical and physiological traits, but were distinguishable from other species of the genus Labrys. Hence, the description of a novel species in this genus appears to be justified. The name Labrys wisconsinensis sp. nov. is proposed; the type strain is W1215-PCA4T (=DSM 19619T=NRRL B-51088T).
Bacteroides clarus sp. nov., Bacteroides fluxus sp. nov. and Bacteroides oleiciplenus sp. nov., isolated from human faeces
Authors: Watanabe Yohei , Nagai Fumiko , Morotomi Masami , Sakon Hiroshi , Tanaka Ryuichiro
Published: November 2009

Three Gram-stain-negative, obligately anaerobic, non-spore-forming, rod-shaped bacteria (strains YIT 12056T, YIT 12057T and YIT 12058T) were isolated from human faeces. These strains were characterized by phylogenetic analyses based on 16S rRNA gene sequence and phenotypic tests. 16S rRNA gene sequence analyses revealed that strains YIT 12056T, YIT 12057T and YIT 12058T were most closely related to the type strains of Bacteroides gallinarum, Bacteroides uniformis and Bacteroides intestinalis with approximate similarity values of 96.6, 95.0 and 96.7 %, respectively. The DNA G+C contents of the novel strains were 45.3 (YIT 12056T), 45.2 (YIT 12057T) and 43.6 mol% (YIT 12058T) and the major respiratory quinones of all three isolates were menaquinones MK-10 and MK-11. These properties were typical for members of the genus Bacteroides. The results of the other phenotypic analyses also supported the affiliation of these strains to the genus Bacteroides. The 16S rRNA gene sequence analysis, analysis of the major cellular fatty acids and other biochemical tests enabled the genotypic and phenotypic differentiation of the three new strains. Based on these data, three novel species, Bacteroides clarus sp. nov., Bacteroides fluxus sp. nov. and Bacteroides oleiciplenus sp. nov. are proposed. The type strains of B. clarus, B. fluxus and B. oleiciplenus are YIT 12056T (=JCM 16067T=DSM 22519T), YIT 12057T (=JCM 16101T=DSM 22534T) and YIT 12058T (=JCM 16102T=DSM 22535T), respectively.
Paenibacillus glacialis sp. nov., isolated from the Kafni glacier of the Himalayas, India
Authors: Kishore K. Hara , Begum Zareena , Pathan Akbar Ali Khan , Shivaji S.
Published: November 2009

A novel strain of the genusPaenibacillus, KFC91T, was isolated from the Kafni glacier of the Himalayas. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KFC91Tclustered withPaenibacillus antarcticusLMG 22078T(98.9 %) andPaenibacillus macquariensisLMG 6935T(98.7 %). The cell wall peptidoglycan contained meso-diaminopimelic acid as the diamino acid, anteiso-C15 : 0as the predominant fatty acid and MK-7 as the major isoprenoid quinone. Based on the phylogenetic analysis, chemotaxonomic characteristics and other phenotypic traits, strain KFC91Twas assigned to the genusPaenibacillus. DNA–DNA hybridization experiments of KFC91TwithP. antarcticusandP. macquariensisshowed reassociation values of 39 and 52 %, respectively. Thus, it is proposed that strain KFC91Tshould be assigned the status of a novel species of the genusPaenibacillusand the name proposed isPaenibacillus glacialissp. nov., with KFC91T(=NCCB 100252T=DSM 22343T) as the type strain.
Natronorubrum sediminis sp. nov., an archaeon isolated from a saline lake
Authors: Gutiérrez M. C. , Castillo A. M. , Corral P. , Minegishi H. , Ventosa A.
Published: November 2009

Two novel haloalkaliphilic archaea, strains CG-6T and CG-4, were isolated from sediment of the hypersaline Lake Chagannor in Inner Mongolia, China. Cells of the two strains were pleomorphic, non-motile and strictly aerobic. They required at least 2.5 M NaCl for growth, with optimum growth at 3.4 M NaCl. They grew at pH 8.0–11.0, with optimum growth at pH 9.0. Hypotonic treatment with less than 1.5 M NaCl caused cell lysis. The two strains had similar polar lipid compositions, possessing C20C20 and C20C25 derivatives of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. No glycolipids were detected. Comparison of 16S rRNA gene sequences and morphological features placed them in the genus Natronorubrum. 16S rRNA gene sequence similarities to strains of recognized species of the genus Natronorubrum were 96.2–93.8 %. Detailed phenotypic characterization and DNA–DNA hybridization studies revealed that the two strains belong to a novel species in the genus Natronorubrum, for which the name Natronorubrum sediminis sp. nov. is proposed; the type strain is CG-6T (=CECT 7487T =CGMCC 1.8981T =JCM 15982T).
Amorphus orientalis sp. nov., an exopolysaccharide-producing bacterium isolated from salt mine sediment
Authors: Wang Yong-Xia , Liu Ji-Hui , Chen Yi-Guang , Zhang Xiao-Xia , Wang Zhi-Gang , Chen Yun , Tian Shi-Ping , Hu Bin , Cui Xiao-Long
Published: November 2009

A Gram-negative, moderately halophilic, non-motile, aerobic bacterium, designated strain YIM D10T, was isolated from a salt mine sediment sample from Yunnan, south-west China. The strain grew optimally in the presence of 3–8 % NaCl and at 28 °C and pH 7.5. The polar lipid profile of strain YIM D10T comprised diphosphatidylglycerol, an unknown phospholipid and two unknown aminolipids. The major cellular fatty acids were C18 : 1
ω7c (30.5 %), C19 : 0 cyclo ω8c (29.3 %) and C18 : 0 (13.2 %). The respiratory quinone was ubiquinone 10 (Q-10). The genomic DNA G+C content was 65.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM D10T was closely related to Amorphus coralli RS.Sph.026T (96.8 % gene sequence similarity). Results confirmed the placement of isolate YIM D10T within the genus Amorphus. However, DNA–DNA hybridization between strain YIM D10T and the type strain of the only recognized species of the genus Amorphus, A. coralli RS.Sph.026T, was 16.7 %, showing clearly that the isolate constitutes a new genospecies. Strain YIM D10T could be clearly differentiated from A. coralli and other phylogenetic neighbours on the basis of some phenotypic, genotypic and chemotaxonomic features. Therefore, strain YIM D10T is considered to represent a novel species of the genus Amorphus, for which the name Amorphus orientalis sp. nov. is proposed; the type strain is YIM D10T (=DSM 21202T=CCTCC AA 208035T).
Haladaptatus litoreus sp. nov., an extremely halophilic archaeon from a marine solar saltern, and emended description of the genus Haladaptatus
Authors: Cui Heng-Lin , Sun Fang-Fang , Gao Xia , Dong Ying , Xu Xue-Wei , Zhou Yu-Guang , Liu Hong-Can , Oren Aharon , Zhou Pei-Jin
Published: November 2009

Two extremely halophilic archaea, strains RO1-28T and RO1-22, were isolated from a marine solar saltern in Jiangsu, China. Both strains required at least 0.05 M Mg2+ and 1.7 M NaCl for growth. They were able to grow over a pH range of 6.0–8.5 and a temperature range of 25–55 °C, with optimal pH of 7.0 and optimal temperature of 37–40 °C. Based on 16S rRNA gene sequence analysis, strains RO1-28T and RO1-22 were closely related to Haladaptatus paucihalophilus, the single species of the genus Haladaptatus, with similarities of 94.0–95.2 %. The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and three glycolipids chromatographically identical to the glycolipids of Haladaptatus paucihalophilus JCM 13897T. Both strains RO1-28T and RO1-22 had a DNA G+C content of 54.0 mol% (HPLC). The DNA–DNA hybridization value between the two strains was more than 70 % (92 %) and both strains showed low levels of DNA–DNA relatedness (32 % and 33 %) with Haladaptatus paucihalophilus JCM 13897T. It was concluded that strains RO1-28T and RO1-22 represent a novel species of the genus Haladaptatus, for which the name Haladaptatus litoreus sp. nov. is proposed. The type strain is RO1-28T (=CGMCC 1.7737T =JCM 15771T).