bluuebunny/crossref_metadata_2025 huggingface dataset:
Chagas Disease: Perspectives on the Past and Present and Challenges in Drug Discovery
Authors: Mansoldo Felipe Raposo Passos , Carta Fabrizio , Angeli Andrea , Cardoso Veronica da Silva , Supuran Claudiu T. , Vermelho Alane Beatriz
Published: November 2020

Chagas disease still has no effective treatment option for all of its phases despite being discovered more than 100 years ago. The development of commercial drugs has been stagnating since the 1960s, a fact that sheds light on the question of how drug discovery research has progressed and taken advantage of technological advances. Could it be that technological advances have not yet been sufficient to resolve this issue or is there a lack of protocol, validation and standardization of the data generated by different research teams? This work presents an overview of commercial drugs and those that have been evaluated in studies and clinical trials so far. A brief review is made of recent target-based and phenotypic studies based on the search for molecules with anti-Trypanosoma cruzi action. It also discusses how proteochemometric (PCM) modeling and microcrystal electron diffraction (MicroED) can help in the case of the lack of a 3D protein structure; more specifically, Trypanosoma cruzi carbonic anhydrase.
Antiproliferative Homoleptic and Heteroleptic Phosphino Silver(I) Complexes: Effect of Ligand Combination on Their Biological Mechanism of Action
Authors: Dammak Khouloud , Porchia Marina , De Franco Michele , Zancato Mirella , Naïli Houcine , Gandin Valentina , Marzano Cristina
Published: November 2020

A series of neutral mixed-ligand [HB(pz)3]Ag(PR3) silver(I) complexes (PR3 = tertiary phosphine, [HB(pz)3]− = tris(pyrazolyl)borate anion), and the corresponding homoleptic [Ag(PR3)4]BF4 compounds have been synthesized and fully characterized. Silver compounds were screened for their antiproliferative activities against a wide panel of human cancer cells derived from solid tumors and endowed with different platinum drug sensitivity. Mixed-ligand complexes were generally more effective than the corresponding homoleptic derivatives, but the most active compounds were [HB(pz)3]Ag(PPh3) (5) and [Ag(PPh3)4]BF4 (10), both comprising the lipophilic PPh3 phosphine ligand. Detailed mechanistic studies revealed that both homoleptic and heteroleptic silver complexes strongly and selectively inhibit the selenoenzyme thioredoxin reductase both as isolated enzyme and in human ovarian cancer cells (half inhibition concentration values in the nanomolar range) causing the disruption of cellular thiol-redox homeostasis, and leading to apoptotic cell death. Moreover, for heteroleptic Ag(I) derivatives, an additional ability to damage nuclear DNA has been detected. These results confirm the importance of the type of silver ion coordinating ligands in affecting the biological behavior of the overall corresponding silver complexes, besides in terms of hydrophilic–lipophilic balance, also in terms of biological mechanism of action, such as interaction with DNA and/or thioredoxin reductase.
Gas Sensing with Solar Cells: The Case of NH3 Detection through Nanocarbon/Silicon Hybrid Heterojunctions
Authors: Drera Giovanni , Freddi Sonia , Freddi Tiziano , De Poli Andrea , Pagliara Stefania , De Crescenzi Maurizio , Castrucci Paola , Sangaletti Luigi
Published: November 2020

Photovoltaic (PV) cells based on single-walled carbon nanotube (SWCNT)/silicon (Si) and multiwalled carbon nanotube (MWCNT)/Si junctions were tested under exposure to NH3 in the 0–21 ppm concentration range. The PV cell parameters remarkably changed upon NH3 exposure, suggesting that these junctions, while being operated as PV cells, can react to changes in the environment, thereby acting as NH3 gas sensors. Indeed, by choosing the open-circuit voltage, VOC, parameter as read-out, it was found that these cells behaved as gas sensors, operating at room temperature with a response higher than chemiresistors developed on the same layers. The sensitivity was further increased when the whole current–voltage (I–V) curve was collected and the maximum power values were tracked upon NH3 exposure.
CXCL12-PLGA/Pluronic Nanoparticle Internalization Abrogates CXCR4-Mediated Cell Migration
Authors: Pisani Anissa , Donno Roberto , Gennari Arianna , Cibecchini Giulia , Catalano Federico , Marotta Roberto , Pompa Pier Paolo , Tirelli Nicola , Bardi Giuseppe
Published: November 2020

Chemokine-induced chemotaxis mediates physiological and pathological immune cell trafficking, as well as several processes involving cell migration. Among them, the role of CXCL12/CXCR4 signaling in cancer and metastasis is well known, and CXCR4 has been often targeted with small molecule-antagonists or short CXCL12-derived peptides to limit the pathological processes of cell migration and invasion. To reduce CXCR4-mediated chemotaxis, we adopted a different approach. We manufactured poly(lactic acid-co-glycolic acid) (PLGA)/Pluronic F127 nanoparticles through microfluidics-assisted nanoprecipitation and functionalized them with streptavidin to docking a biotinylated CXCL12 to be exposed on the nanoparticle surface. Our results show that CXCL12-decorated nanoparticles are non-toxic and do not induce inflammatory cytokine release in THP-1 monocytes cultured in fetal bovine and human serum-supplemented media. The cell internalization of our chemokine receptor-targeting particles increases in accordance with CXCR4 expression in FBS/medium. We demonstrated that CXCL12-decorated nanoparticles do not induce cell migration on their own, but their pre-incubation with THP-1 significantly decreases CXCR4+-cell migration, thereby antagonizing the chemotactic action of CXCL12. The use of biodegradable and immune-compatible chemokine-mimetic nanoparticles to reduce cell migration opens the way to novel antagonists with potential application in cancer treatments and inflammation.
Making van der Waals Heterostructures Assembly Accessible to Everyone
Authors: Martanov Sergey G. , Zhurbina Natalia K. , Pugachev Mikhail V. , Duleba Aliaksandr I. , Akmaev Mark A. , Belykh Vasilii V. , Kuntsevich Aleksandr Y.
Published: November 2020

Van-der Waals heterostructures assembled from one or few atomic layer thickness crystals are becoming increasingly more popular in condensed matter physics. These structures are assembled using transfer machines, those are based on mask aligners, probe stations or are home-made. For many laboratories it is vital to build a simple, convenient and universal transfer machine. In this paper we discuss the guiding principles for the design of such a machine, review the existing machines and demonstrate our own construction, that is powerful and fast-in-operation. All components of this machine are extremely cheap and can be easily purchased using common online retail services. Moreover, assembling a heterostructure out of exfoliated commercially available hexagonal boron nitride and tungsten diselenide crystals with a pick-up technique and using the microphotolumenescence spectra, we show well-resolved exciton and trion lines, as a results of disorder suppression in WSe2 monolayer. Our results thus show that technology of the two-dimensional materials and heterostructures becomes accessible to anyone.
Treatment of Breast Cancer-Bearing BALB/c Mice with Magnetic Hyperthermia using Dendrimer Functionalized Iron-Oxide Nanoparticles
Authors: Salimi Marzieh , Sarkar Saeed , Hashemi Mansoureh , Saber Reza
Published: November 2020

The development of novel nanoparticles for diagnostic and therapeutic applications has been one of the most crucial challenges in cancer theranostics for the last decades. Herein, we functionalized iron oxide nanoparticles (IONPs) with the fourth generation (G4) of poly amidoamine (PAMAM) dendrimers (G4@IONPs) for magnetic hyperthermia treatment of breast cancer in Bagg albino strain C (BALB/c)mice. The survival of breast cancer cells significantly decreased after incubation with G4@IONPs and exposure to an alternating magnetic field (AMF) due to apoptosis and elevation of Bax (Bcl-2 associated X)/Bcl-2(B-cell lymphoma 2) ratio. After intratumoral injection of G4@IONPs, tumor-bearing BALB/c mice were exposed to AMF for 20 min; this procedure was repeated three times every other day. After the last treatment, tumor size was measured every three days. Histopathological and Immunohistochemical studies were performed on the liver, lung, and tumor tissues in treated and control mice. The results did not show any metastatic cells in the liver and lung tissues in the treatment group, while the control mice tissues contained metastatic breast cancer cells. Furthermore, the findings of the present study showed that magnetic hyperthermia treatment inhibited tumor growth by increasing cancer cell apoptosis, as well as reducing the tumor angiogenesis.
Supramolecular Porphyrin Nanostructures Based on Coordination-Driven Self-Assembly and Their Visible Light Catalytic Degradation of Methylene Blue Dye
Authors: Shee Nirmal Kumar , Kim Min Kyoung , Kim Hee-Joon
Published: November 2020

A series of porphyrin triads (1–4), in which each triad is composed of a Sn(IV) porphyrin and two free-base (or Zn(II)) porphyrins, was synthesized and their self-assembled nanostructures were studied. Depending on the substituent on porphyrin moieties, each triad was self-assembled into a different nanostructure. In particular, the cooperative coordination of 3-pyridyl groups in the Sn(IV) porphyrin with the axial Zn(II) porphyrins in triad 4 leads to forming uniform nanofibers with an average width of 10–22 nm. Other triads without the coordinating interaction between the central Sn(IV) porphyrin and the axial porphyrins formed irregularly shaped aggregates in contrast. The morphologies of nanofiber changed drastically upon the addition of pyrrolidine, in which pyrrolidine molecules break down the self-assembly process by coordinating with the axial Zn(II) porphyrins. All porphyrin aggregates exhibited efficient photocatalytic performances on the degradation of methylene blue dye under visible light irradiation. The degradation efficiencies after 2 h were observed to be between 70% and 95% for the aggregates derived from the four triads.
A Review of Carbon Dots Produced from Biomass Wastes
Authors: Kang Chao , Huang Ying , Yang Hui , Yan Xiu Fang , Chen Zeng Ping
Published: November 2020

The fluorescent carbon dot is a novel type of carbon nanomaterial. In comparison with semiconductor quantum dots and fluorescence organic agents, it possesses significant advantages such as excellent photostability and biocompatibility, low cytotoxicity and easy surface functionalization, which endow it a wide application prospect in fields of bioimaging, chemical sensing, environmental monitoring, disease diagnosis and photocatalysis as well. Biomass waste is a good choice for the production of carbon dots owing to its abundance, wide availability, eco-friendly nature and a source of low cost renewable raw materials such as cellulose, hemicellulose, lignin, carbohydrates and proteins, etc. This paper reviews the main sources of biomass waste, the feasibility and superiority of adopting biomass waste as a carbon source for the synthesis of carbon dots, the synthetic approaches of carbon dots from biomass waste and their applications. The advantages and deficiencies of carbon dots from biomass waste and the major influencing factors on their photoluminescence characteristics are summarized and discussed. The challenges and perspectives in the synthesis of carbon dots from biomass wastes are also briefly outlined.
Multilocus Genotyping Reveals New Molecular Markers for Differentiating Distinct Genetic Lineages among “Candidatus Phytoplasma Solani” Strains Associated with Grapevine Bois Noir
Authors: Passera Alessandro , Zhao Yan , Murolo Sergio , Pierro Roberto , Arsov Emilija , Mori Nicola , Moussa Abdelhameed , Silletti Maria R. , Casati Paola , Panattoni Alessandra , Wei Wei , Mitrev Sasa , Materazzi Alberto , Luvisi Andrea , Romanazzi Gianfranco , Bianco Piero A. , Davis Robert E. , Quaglino Fabio
Published: November 2020

Grapevine Bois noir (BN) is associated with infection by “Candidatus Phytoplasma solani” (CaPsol). In this study, an array of CaPsol strains was identified from 142 symptomatic grapevines in vineyards of northern, central, and southern Italy and North Macedonia. Molecular typing of the CaPsol strains was carried out by analysis of genes encoding 16S rRNA and translation elongation factor EF-Tu, as well as eight other previously uncharacterized genomic fragments. Strains of tuf-type a and b were found to be differentially distributed in the examined geographic regions in correlation with the prevalence of nettle and bindweed. Two sequence variants were identified in each of the four genomic segments harboring hlyC, cbiQ-glyA, trxA-truB-rsuA, and rplS-tyrS-csdB, respectively. Fifteen CaPsol lineages were identified based on distinct combinations of sequence variations within these genetic loci. Each CaPsol lineage exhibited a unique collective restriction fragment length polymorphism (RFLP) pattern and differed from each other in geographic distribution, probably in relation to the diverse ecological complexity of vineyards and their surroundings. This RFLP-based typing method could be a useful tool for investigating the ecology of CaPsol and the epidemiology of its associated diseases. Phylogenetic analyses highlighted that the sequence variants of the gene hlyC, which encodes a hemolysin III-like protein, separated into two clusters consistent with the separation of two distinct lineages on the basis of tufB gene sequences. Alignments of deduced full protein sequences of elongation factor-Tu (tufB gene) and hemolysin III-like protein (hlyC gene) revealed the presence of critical amino acid substitutions distinguishing CaPsol strains of tuf-type a and b. Findings from the present study provide new insights into the genetic diversity and ecology of CaPsol populations in vineyards.
Infection with Helicobacter pylori Induces Epithelial to Mesenchymal Transition in Human Cholangiocytes
Authors: Thanaphongdecha Prissadee , Karinshak Shannon E. , Ittiprasert Wannaporn , Mann Victoria H. , Chamgramol Yaovalux , Pairojkul Chawalit , Fox James G. , Suttiprapa Sutas , Sripa Banchob , Brindley Paul J.
Published: November 2020

Recent reports suggest that the East Asian liver fluke infection, caused by Opisthorchis viverrini, which is implicated in opisthorchiasis-associated cholangiocarcinoma, serves as a reservoir of Helicobacter pylori. The opisthorchiasis-affected cholangiocytes that line the intrahepatic biliary tract are considered to be the cell of origin of this malignancy. Here, we investigated interactions in vitro among human cholangiocytes, Helicobacter pylori strain NCTC 11637, and the congeneric bacillus, Helicobacter bilis. Exposure to increasing numbers of H. pylori at 0, 1, 10, 100 bacilli per cholangiocyte of the H69 cell line induced phenotypic changes including the profusion of thread-like filopodia and a loss of cell-cell contact, in a dose-dependent fashion. In parallel, following exposure to H. pylori, changes were evident in levels of mRNA expression of epithelial to mesenchymal transition (EMT)-encoding factors including snail, slug, vimentin, matrix metalloprotease, zinc finger E-box-binding homeobox, and the cancer stem cell marker CD44. Analysis to quantify cellular proliferation, migration, and invasion in real-time by both H69 cholangiocytes and CC-LP-1 line of cholangiocarcinoma cells using the xCELLigence approach and Matrigel matrix revealed that exposure to ≥10 H. pylori bacilli per cell stimulated migration and invasion by the cholangiocytes. In addition, 10 bacilli of H. pylori stimulated contact-independent colony establishment in soft agar. These findings support the hypothesis that infection by H.pylori contributes to the malignant transformation of the biliary epithelium.
The Functional Parasitic Worm Secretome: Mapping the Place of Onchocerca volvulus Excretory Secretory Products
Authors: Vanhamme Luc , Souopgui Jacob , Ghogomu Stephen , Ngale Njume Ferdinand
Published: November 2020

Nematodes constitute a very successful phylum, especially in terms of parasitism. Inside their mammalian hosts, parasitic nematodes mainly dwell in the digestive tract (geohelminths) or in the vascular system (filariae). One of their main characteristics is their long sojourn inside the body where they are accessible to the immune system. Several strategies are used by parasites in order to counteract the immune attacks. One of them is the expression of molecules interfering with the function of the immune system. Excretory-secretory products (ESPs) pertain to this category. This is, however, not their only biological function, as they seem also involved in other mechanisms such as pathogenicity or parasitic cycle (molting, for example). We will mainly focus on filariae ESPs with an emphasis on data available regarding Onchocerca volvulus, but we will also refer to a few relevant/illustrative examples related to other worm categories when necessary (geohelminth nematodes, trematodes or cestodes). We first present Onchocerca volvulus, mainly focusing on the aspects of this organism that seem relevant when it comes to ESPs: life cycle, manifestations of the sickness, immunosuppression, diagnosis and treatment. We then elaborate on the function and use of ESPs in these aspects.
Stability of African Swine Fever Virus in Soil and Options to Mitigate the Potential Transmission Risk
Authors: Carlson Jolene , Fischer Melina , Zani Laura , Eschbaumer Michael , Fuchs Walter , Mettenleiter Thomas , Beer Martin , Blome Sandra
Published: November 2020

Understanding African swine fever virus (ASFV) transmission is essential for strategies to minimize virus spread during an outbreak. ASFV can survive for extended time periods in animal products, carcasses, and the environment. While the ASFV genome was found in environments around infected farms, data on the virus survival in soil are scarce. We investigated different soil matrices spiked with ASFV-positive blood from infected wild boar to see if ASFV can remain infectious in the soil beneath infected carcasses. As expected, ASFV genome detection was possible over the entire sampling period. Soil pH, structure, and ambient temperature played a role in the stability of infectious ASFV. Infectious ASFV was demonstrated in specimens originating from sterile sand for at least three weeks, from beach sand for up to two weeks, from yard soil for one week, and from swamp soil for three days. The virus was not recovered from two acidic forest soils. All risk mitigation experiments with citric acid or calcium hydroxide resulted in complete inactivation. In conclusion, the stability of infectious ASFV is very low in acidic forest soils but rather high in sandy soils. However, given the high variability, treatment of carcass collection points with disinfectants should be considered.
An Outbreak of Hepatitis A among Young Adult Men in Cyprus
Authors: Dimitriou Panagiotis , Nikolopoulos Georgios K. , Koliou Maria , Constantinou Elisavet , Azina Chara , Panayiotou Maria , Christaki Eirini
Published: November 2020

Background: Outbreaks of acute hepatitis A (AHA) have recently been reported in Europe among men who have sex with men (MSM). The aim of this work was to evaluate, for the first time, trends in the reported cases of AHA in Cyprus over the last seven years. Methods: We retrospectively studied all people reported with AHA in Cyprus between January 2013 and December 2019. Demographic data, type of transmission, vaccination status for HAV, laboratory and clinical data were analyzed. Results: The asnalysis involved 33 AHA cases (age 32.7 ± 17.4 years, 78.8% males). An increase in AHA reports was observed between July 2017 and June 2018 when more than a third (n = 13) of the cases of the period 2013–2019 were reported. The reporting rate of AHA doubled from 0.52 cases per 100,000 population (before July 2017) to 1.12 cases per 100,000 population (July 2017–June 2018). The male/female (M/F) ratio increased from one in 2013 to eight in 2018. Conclusion: An increase in AHA reports occurred in Cyprus between July 2017 and June 2018. Many cases with AHA in that period were MSM. Enhanced surveillance and timely public health interventions, like vaccination and awareness promotion, are important for preventing future outbreaks.
Population Geography and Emergency Contraception Access in Louisiana
Authors: Chiccarelli Elvira , Khorsandi Nikka , Dumas S. Amanda , Aden James K. , Pasternak Ryan H.
Published: November 2020

We assessed the same-day availability of oral emergency contraception (EC) in five Louisiana communities, and evaluated this data for relationships between availability and local population demographics. Researchers called all retail pharmacies in five municipalities of varying sizes in order to inquire about the same-day stockage of EC products and their availability to teens. Individual pharmacies were then geolocated to a census tract, and call data was analyzed against neighborhood census data regarding population size, income, gender, race, family structure, and educational level. A multivariable logistic regression model was performed to predict the same-day availability of emergency contraception. EC was available on the same day in 66% of all pharmacies. The same-day availability of EC decreased with the local population size (p < 0.001), and the availability increased with higher levels of educational attainment (p = 0.0015). The largest census level predictor of access to same-day EC was the city population, with the availability increasing by 6.6% for every 10,000 person increase in population. Despite changing to over-the-counter sales in 2013, EC is still not widely available in all geographic areas. Its availability is partially predictable by local population demographics, and this difference may represent a health disparity for teens and women seeking EC.
Tomato Brown Rugose Fruit Virus: Seed Transmission Rate and Efficacy of Different Seed Disinfection Treatments
Authors: Davino Salvatore , Caruso Andrea Giovanni , Bertacca Sofia , Barone Stefano , Panno Stefano
Published: November 2020

Tomato brown rugose fruit virus (ToBRFV) is a highly infectious virus, that is becoming a threat to tomato production worldwide. In this work we evaluated the localization of ToBRFV particles in tomato seeds, its seed transmission rate and efficacy of disinfection, and the effects of different thermal- and chemical-based treatments on ToBRFV-infected seeds’ germination. Analyses demonstrated that ToBRFV was located in the seed coat, sometime in the endosperm, but never in the embryo; its transmission from infected seeds to plantlets occurs by micro-lesions during the germination. The ToBRFV seed transmission rate was 2.8% in cotyledons and 1.8% in the third true leaf. Regarding the different disinfection treatments, they returned 100% of germination at 14 days post-treatment (dpt), except for the treatment with 2% hydrochloric acid +1.5% sodium hypochlorite for 24 h, for which no seed germinated after 14 dpt. All treatments have the ability to inactivate ToBRFV, but in six out of seven treatments ToBRFV was still detectable by RT-qPCR. These results raise many questions about the correct way to carry out diagnosis at customs. To our knowledge, this is the first study on the effective localization of ToBRFV particles in seeds.
Epipactis tremolsii Seed Diversity in Two Close but Extremely Different Populations: Just a Case of Intraspecific Variability?
Authors: De Agostini Antonio , Cortis Pierluigi , Cogoni Annalena , Gargiulo Roberta , Fenu Giuseppe
Published: November 2020

Analysis of the seed morphology is a widely used approach in ecological and taxonomic studies. In this context, intraspecific variability with respect to seed morphology (size, weight, and density) was assessed in two close Epipactis tremolsii Pau. populations sharing the same ecological conditions, except for the soil pollution distinguishing one of them. Larger and heavier seeds were found in plants growing on the heavy metal polluted site, while no differences in seed density were detected between seeds produced by plants growing on the contaminated and the control site. Moreover, seed coats and embryos varying together in their dimensions were described in the control population, while coats varying in their size independently from embryos were described in plants growing on the polluted site. Seeds from the two studied populations significantly differed in several parameters suggesting that intraspecific seed variability occurred in the case study.
Effect of Salinity Stress on Growth and Metabolomic Profiling of Cucumis sativus and Solanum lycopersicum
Authors: Abdel-Farid Ibrahim Bayoumi , Marghany Marwa Radawy , Rowezek Mohamed Mahmoud , Sheded Mohamed Gabr
Published: November 2020

Seeds germination and seedlings growth of Cucumis sativus and Solanum lycopersicum were monitored in in vitro and in vivo experiments after application of different concentrations of NaCl (25, 50, 100 and 200 mM). Photosynthetic pigments content and the biochemical responses of C. sativus and S. lycopersicum were assessed. Salinity stress slightly delayed the seeds germination rate and significantly reduced the percentage of germination as well as shoot length under the highest salt concentration (200 mM) in cucumber. Furthermore, root length was decreased significantly in all treatments. Whereas, in tomato, a prominent delay in seeds germination rate, the germination percentage and seedlings growth (shoot and root lengths) were significantly influenced under all concentrations of NaCl. Fresh and dry weights were reduced prominently in tomato compared to cucumber. Photosynthetic pigments content was reduced but with pronounced decreasing in tomato compared to cucumber. Secondary metabolites profiling in both plants under stress was varied from tomato to cucumber. The content of saponins, proline and total antioxidant capacity was reduced more prominently in tomato as compared to cucumber. On the other hand, the content of phenolics and flavonoids was increased in both plants with pronounced increase in tomato particularly under the highest level of salinity stress. The metabolomic profiling in stressful plants was significantly influenced by salinity stress and some bioactive secondary metabolites was enhanced in both cucumber and tomato plants. The enhancement of secondary metabolites under salinity stress may explain the tolerance and sensitivity of cucumber and tomato under salinity stress. The metabolomic evaluation combined with multivariate data analysis revealed a similar mechanism of action of plants to mediate stress, with variant level of this response in both plant species. Based on these results, the effect of salinity stress on seeds germination, seedlings growth and metabolomic content of plants was discussed in terms of tolerance and sensitivity of plants to salinity stress.
Effects of Different Processing Treatments on Almond (Prunus dulcis) Bioactive Compounds, Antioxidant Activities, Fatty Acids, and Sensorial Characteristics
Authors: Oliveira Ivo , Meyer Anne S. , Afonso Sílvia , Sequeira Alex , Vilela Alice , Goufo Piebiep , Trindade Henrique , Gonçalves Berta
Published: November 2020

Almond is one of the most commonly consumed nuts worldwide, with health benefits associated with availability of bioactive compounds and fatty acids. Almond is often eaten raw or after some processing steps. However, the latter can positively or negatively influence chemical and sensorial attributes of almonds. This work was carried out to assess the effects of two processing treatments, namely; roasting and blanching on (i) contents of bioactive compounds, (ii) contents of fatty acids (3) antioxidant activities (4), sensorial characteristics of four neglected Portuguese almond cultivars (Casanova, Molar, Pegarinhos and Refêgo) and two foreign cultivars (Ferragnès and Glorieta). Results showed that in general, levels of bioactive compounds and antioxidant activities increased with roasting and decreased with blanching. Fatty acid profiles of raw kernels of all cultivars were generally identical although Refêgo exhibited a high content of α-linolenic acid. Following roasting and blanching, content of polyunsaturated fatty acids increased while saturated fatty acids, monounsaturated fatty acids and several health lipid indices decreased. Roasting positively affected perception of skin color and sweetness of Ferragnès and Glorieta as well as skin roughness of Molar and Pegarinhos. Blanching on the other hand led to positive changes in textural properties of Refêgo and Pegarinhos. This study reveals the nutritive benefits of consuming neglected almond cultivars in Portugal, and the novel data reported here could be of interest to growers, processing companies and consumers.
Dog Rabies in Dhaka, Bangladesh, and Implications for Control
Authors: A Masud M , Islam Md Hamidul , Adnan Muhaiminul Islam , Oh Chunyoung
Published: November 2020

Controlling rabies among free-roaming street dogs has been a huge challenge in many parts of the world. Vaccination is a commonly used strategy to control rabies, however, sufficient vaccination coverage is very challenging when it comes to street dogs. Also, dog rabies data is scarce, making it difficult to develop proper strategies. In this study, we use a logistic growth incorporated epidemic model to understand the prevalence of rabies in the dog population of Dhaka, Bangladesh. The study shows that, the basic reproduction number for dog rabies in Dhaka lies between 1.1 to 1.249 and the environmental carrying capacity lies approximately between 58,110 to 194,739. Considering the vaccination and neuter programs administered in the last decade, we attempt to explain rabies transmission among dogs in this population. We found that the high basic reproduction number is associated with high environmental carrying capacity and vice versa. Further, we compare different type of control strategies, viz., constant vaccination, pulse vaccination, and optimal vaccination strategies. In the case of high environmental carrying capacity, vaccination, and neuter strategy is not sufficient for controlling rabies in street dogs, whereas carrying capacity control through waste management coupled with vaccination and neuter is more effective.
Hot Melt Extrusion Processing Parameters Optimization
Authors: Alshetaili Abdullah , Alshahrani Saad M. , Almutairy Bjad K. , Repka Michael A.
Published: November 2020

The aim of this study was to demonstrate the impact of processing parameters of the hot-melt extrusion (HME) on the pharmaceutical formulation properties. Carbamazepine (CBZ) was selected as a model water-insoluble drug. It was incorporated into Soluplus®, which was used as the polymeric carrier, to produce a solid dispersion model system. The following HME-independent parameters were investigated at different levels: extrusion temperature, screw speed and screw configuration. Design of experiment (DOE) concept was applied to find the most significant factor with minimum numbers of experimental runs. A full two-level factorial design was applied to assess the main effects, parameter interactions and total error. The extrudates’ CBZ content and the in vitro dissolution rate were selected as response variables. Material properties, including melting point, glass transition, and thermal stability, and polymorphs changes were used to set the processing range. In addition, the extruder torque and pressure were used to find the simplest DOE model. Each change of the parameter showed a unique pattern of dissolution profile, indicating that processing parameters have an influence on formulation properties. A simple, novel and two-level factorial design was able to evaluate each parameter effect and find the optimized formulation. Screw configuration and extrusion temperature were the most affecting parameters in this study.
Numerical Investigation of the Characteristics of the In-Cylinder Air Flow in a Compression-Ignition Engine for the Application of Emulsified Biofuels
Authors: Hamid Mohd Fadzli , Idroas Mohamad Yusof , Mohamed Mazlan , Sa'ad Shukriwani , Yew Heng Teoh , Che Mat Sharzali , Miskam Muhamad Azman , Abdullah Muhammad Khalil
Published: November 2020

This paper presents a numerical analysis of the application of emulsified biofuel (EB) to diesel engines. The study performs a numerical study of three different guide vane designs (GVD) that are incorporated with a shallow depth re-entrance combustion chamber (SCC) piston. The GVD variables were used in three GVD models with different vane heights, that is, 0.2, 0.4 and 0.6 times the radius of the intake runner (R) and these were named 0.20R, 0.40R and 0.60R. The SCC piston and GVD model were designed using SolidWorks 2017, while ANSYS Fluent version 15 was used to perform cold flow engine 3D analysis. The results of the numerical study showed that 0.60R is the optimum guide vane height, as the turbulence kinetic energy (TKE), swirl ratio (Rs), tumble ratio (RT) and cross tumble ratio (RCT) in the fuel injection region improved from the crank angle before the start of injection (SOI) and start of combustion (SOC). This is essential to break up the heavier-fuel molecules of EB so that they mix with the surrounding air, which eventually improves the engine performance.
Design of Cyclone Separator Critical Diameter Model Based on Machine Learning and CFD
Authors: Park Donggeun , Go Jeung Sang
Published: November 2020

In this paper, the characteristics of the cyclone separator was analyzed from the Lagrangian perspective for designing the important dependent variables. The neural network network model was developed for predicting the separation performance parameter. Further, the predictive performances were compared between the traditional surrogate model and the developed neural network model. In order to design the important parameters of the cyclone separator based on the particle separation theory, the force acting until the particles are separated was calculated using the Lagrangian-based computational fluid dynamics (CFD) methodology. As a result, it was proved that the centrifugal force and drag acting on the critical diameter having a separation efficiency of 50% were similar, and the particle separation phenomenon in the cyclone occurred from the critical diameter, and it was set as an important dependent variable. For developing a critical diameter prediction model based on machine learning and multiple regression methods, unsteady-Reynolds averaged Navier-Stokes analyzes according to shape dimensions were performed. The input design variables for predicting the critical diameter were selected as four geometry parameters that affect the turbulent flow inside the cyclone. As a result of comparing the model prediction performances, the machine learning (ML) model, which takes into account the critical diameter and the nonlinear relationship of cyclone design variables, showed a 32.5% improvement in R-square compared to multi linear regression (MLR). The proposed techniques have proven to be fast and practical tools for cyclone design.
Reporting of Hybrid Data and the Difficulties with Cross-Discipline Research Techniques
Authors: O’Rourke Matthew B. , Padula Matthew P.
Published: November 2020

Peer review is the way in which we, as scientists, criticise, check, and confirm the findings of our colleagues. The process of peer review relies on individuals in all fields applying their particular expertise and determining if they agree with the findings submitted for publication. In recent years, there has been a significant rise in the number of manuscripts submitted for publication that draw from a range of disparate and complementary fields. This has created the curious situation where an expert may be requested to review a manuscript that is only partially within their immediate field of expertise. The issue that arises is that, without full knowledge of the data, techniques, methodologies, and principles that are presented, it is difficult for reviewers to make properly informed decisions, especially when it can take an entire career to reach that specific level of expertise in a single field. From this perspective, we explore these issues and also provide a commentary on how peer review could evolve in the context of a changing cross-disciplinarily-focused scientific landscape.
Effects of Orientation and Appearance of a Synchronously Moving Object on Hand Movements
Authors: Shibuya Satoshi
Published: November 2020

Various devices have been developed to enable humans to control remote objects using active hand movements. However, it is still unclear how the visual characteristics of a synchronously moving object influences hand movements. This study investigates the effects of visual appearance and orientation of a hand-controlled object on hand movements using a novel visuomotor task. The motion of a visual image on a monitor reflected the participants’ right hand movements in the forwards-backwards direction, but not in the lateral direction (i.e., the lateral position of the image was fixed). Participants performed continuous goal-directed back and forth movements of the visual image for one minute. The image’s appearance (hand and arrow) and orientation (forward (FW), leftward (LW), and rightward (RW)) were manipulated. Unconscious lateral deviations (i.e., drift movements) of the participant’s hand during the task were evaluated. Regardless of appearance, the leftward and rightward image induced leftward and rightward drift movements, compared to the forward image. However, the modulation sizes were similar using arrow images, but not using hand images. Specifically, anatomically plausible hand images elicited greater drift movements than anatomically implausible images. This suggests that both orientation and appearance of a hand-controlled object influences hand movements according to stimulus-response compatibility and body-representation changes.
The Views on Terrorism in the Name of Islam Held by Islamic Religion Teachers in Spain
Authors: Navarro-Granados María , Llorent-Bedmar Vicente , Cobano-Delgado Palma Verónica C.
Published: November 2020

Violent radicalisation is currently one of the most pressing global problems. Accordingly, the intention of this paper is to discover the views on terrorism in the name of Islam held by Islamic religion teachers in Spain and to analyse the preventive socio-educational measures that they propose, employing a mixed methods approach with a questionnaire and semi-structured interviews. Most of the teachers point to a poor knowledge of Islam among the young, especially as regards to the concept of jihad, as the main reason behind this phenomenon. The second-generation immigrants among the teachers attach great importance to possible identity crises as a factor that makes the young more vulnerable to violent radicalisation. The most frequently mentioned social measure is fostering the integration of Muslims, thus creating a sense of belonging to their country of residence. They recommend preventive educational strategies that, far from focusing on detecting possible violent extremists, place the accent on teaching the fundamentals of the Islamic religion in order to provide young people with the tools that they need to challenge violent ideologies.
Specific Features of Mapping Large Discontinuous Faults by the Method of Electromagnetic Emission
Authors: Iakovleva Emiliia , Belova Margarita , Soares Amilcar
Published: November 2020

In this paper, the authors consider the issue of processing the data of electromagnetic pulses (EMPs) recorded in mine workings and their interpretation. The Sami fault (Russia, the Murmansk region) was chosen as the object of the research. A number of experiments, including measuring the EMP level along the fault and the analysis of the results, were performed by the authors. The paper also presents a previously unconsidered method of transition from geological concepts to electro-technical ones, the aim of which is to identify the dependences of the amplitude of electromagnetic field anomalies on the parameters of active tectonic disturbances. The authors assumed the dependence of the amplitude of anomalies on the geological parameters of the rock mass and the thickness of the tectonic disturbance.
Localization and Mapping for Robots in Agriculture and Forestry: A Survey
Authors: Aguiar André Silva , dos Santos Filipe Neves , Cunha José Boaventura , Sobreira Héber , Sousa Armando Jorge
Published: November 2020

Research and development of autonomous mobile robotic solutions that can perform several active agricultural tasks (pruning, harvesting, mowing) have been growing. Robots are now used for a variety of tasks such as planting, harvesting, environmental monitoring, supply of water and nutrients, and others. To do so, robots need to be able to perform online localization and, if desired, mapping. The most used approach for localization in agricultural applications is based in standalone Global Navigation Satellite System-based systems. However, in many agricultural and forest environments, satellite signals are unavailable or inaccurate, which leads to the need of advanced solutions independent from these signals. Approaches like simultaneous localization and mapping and visual odometry are the most promising solutions to increase localization reliability and availability. This work leads to the main conclusion that, few methods can achieve simultaneously the desired goals of scalability, availability, and accuracy, due to the challenges imposed by these harsh environments. In the near future, novel contributions to this field are expected that will help one to achieve the desired goals, with the development of more advanced techniques, based on 3D localization, and semantic and topological mapping. In this context, this work proposes an analysis of the current state-of-the-art of localization and mapping approaches in agriculture and forest environments. Additionally, an overview about the available datasets to develop and test these approaches is performed. Finally, a critical analysis of this research field is done, with the characterization of the literature using a variety of metrics.
Efficient Deployment with Throughput Maximization for UAVs Communication Networks
Authors: Sayeed Mohd Abuzar , Kumar Rajesh , Sharma Vishal , Sayeed Mohd Asim
Published: November 2020

The article presents a throughput maximization approach for UAV assisted ground networks. Throughput maximization involves minimizing delay and packet loss through UAV trajectory optimization, reinforcing the congested nodes and transmission channels. The aggressive reinforcement policy is achieved by characterizing nodes, links, and overall topology through delay, loss, throughput, and distance. A position-aware graph neural network (GNN) is used for characterization, prediction, and dynamic UAV trajectory enhancement. To establish correctness, the proposed approach is validated against optimized link state routing (OLSR) driven UAV assisted ground networks. The proposed approach considerably outperforms the classical approach by demonstrating significant gains in throughput and packet delivery ratio with notable decrements in delay and packet loss. The performance analysis of the proposed approach against software-defined UAVs (U-S) and UAVs as base stations (U-B) verifies the consistency and gains in average throughput while minimizing delay and packet loss. The scalability test of the proposed approach is performed by varying data rates and the number of UAVs.
Single Image Dehazing Algorithm Analysis with Hyperspectral Images in the Visible Range
Authors: Martínez-Domingo Miguel Ángel , Valero Eva M. , Nieves Juan L. , Molina-Fuentes Pedro Jesús , Romero Javier , Hernández-Andrés Javier
Published: November 2020

In foggy or hazy conditions, images are degraded due to the scattering and attenuation of atmospheric particles, reducing the contrast and visibility and changing the color. This degradation depends on the distance, the density of the atmospheric particles and the wavelength. We have tested and applied five single image dehazing algorithms, originally developed to work on RGB images and not requiring user interaction and/or prior knowledge about the images, on a spectral hazy image database in the visible range. We have made the evaluation using two strategies: the first is based on the analysis of eleven state-of-the-art metrics and the second is two psychophysical experiments with 126 subjects. Our results suggest that the higher the wavelength within the visible range is, the higher the quality of the dehazed images. The quality increases for low haze/fog levels. The choice of the best performing algorithm depends on the criterion prioritized by the metric design strategy. The psychophysical experiment results show that the level of agreement between observers and metrics depends on the criterion set for the observers’ task.
Q-Learning Based Joint Energy-Spectral Efficiency Optimization in Multi-Hop Device-to-Device Communication
Authors: Khan Muhidul Islam , Reggiani Luca , Alam Muhammad Mahtab , Le Moullec Yannick , Sharma Navuday , Yaacoub Elias , Magarini Maurizio
Published: November 2020

In scenarios, like critical public safety communication networks, On-Scene Available (OSA) user equipment (UE) may be only partially connected with the network infrastructure, e.g., due to physical damages or on-purpose deactivation by the authorities. In this work, we consider multi-hop Device-to-Device (D2D) communication in a hybrid infrastructure where OSA UEs connect to each other in a seamless manner in order to disseminate critical information to a deployed command center. The challenge that we address is to simultaneously keep the OSA UEs alive as long as possible and send the critical information to a final destination (e.g., a command center) as rapidly as possible, while considering the heterogeneous characteristics of the OSA UEs. We propose a dynamic adaptation approach based on machine learning to improve a joint energy-spectral efficiency (ESE). We apply a Q-learning scheme in a hybrid fashion (partially distributed and centralized) in learner agents (distributed OSA UEs) and scheduler agents (remote radio heads or RRHs), for which the next hop selection and RRH selection algorithms are proposed. Our simulation results show that the proposed dynamic adaptation approach outperforms the baseline system by approximately 67% in terms of joint energy-spectral efficiency, wherein the energy efficiency of the OSA UEs benefit from a gain of approximately 30%. Finally, the results show also that our proposed framework with C-RAN reduces latency by approximately 50% w.r.t. the baseline.
Mobile Manipulation Integrating Enhanced AMCL High-Precision Location and Dynamic Tracking Grasp
Authors: Zhou Huaidong , Chou Wusheng , Tuo Wanchen , Rong Yongfeng , Xu Song
Published: November 2020

Mobile manipulation, which has more flexibility than fixed-base manipulation, has always been an important topic in the field of robotics. However, for sophisticated operation in complex environments, efficient localization and dynamic tracking grasp still face enormous challenges. To address these challenges, this paper proposes a mobile manipulation method integrating laser-reflector-enhanced adaptive Monte Carlo localization (AMCL) algorithm and a dynamic tracking and grasping algorithm. First, by fusing the information of laser-reflector landmarks to adjust the weight of particles in AMCL, the localization accuracy of mobile platforms can be improved. Second, deep-learning-based multiple-object detection and visual servo are exploited to efficiently track and grasp dynamic objects. Then, a mobile manipulation system integrating the above two algorithms into a robotic with a 6-degrees-of-freedom (DOF) operation arm is implemented in an indoor environment. Technical components, including localization, multiple-object detection, dynamic tracking grasp, and the integrated system, are all verified in real-world scenarios. Experimental results demonstrate the efficacy and superiority of our method.