bluuebunny/crossref_metadata_2025 huggingface dataset:
On the Extended Simple Equations Method (SEsM) and Its Application for Finding Exact Solutions of the Time-Fractional Diffusive Predator–Prey System Incorporating an Allee Effect
Authors: Nikolova Elena V.
Published: January 2025

In this paper, I extend the Simple Equations Method (SEsM) and adapt it to obtain exact solutions of systems of fractional nonlinear partial differential equations (FNPDEs). The novelty in the extended SEsM algorithm is that, in addition to introducing more simple equations in the construction of the solutions of the studied FNPDEs, it is assumed that the selected simple equations have different independent variables (i.e., different coordinates moving with the wave). As a consequence, nonlinear waves propagating with different wave velocities will be observed. Several scenarios of the extended SEsM are applied to the time-fractional predator–prey model under the Allee effect. Based on this, new analytical solutions are derived. Numerical simulations of some of these solutions are presented, adequately capturing the expected diverse wave dynamics of predator–prey interactions.
Investigation of the Acute Effects of Two Different Preoxygenation Methods on Neurodegenerative Biomarkers in Laparoscopic Cholecystectomy Surgery
Authors: Pehlivan Veli Fahri , Pehlivan Basak , Celik Hakim , Duran Erdogan , Taskın Abdullah , Taskın Seyhan , Tatlı Faik
Published: January 2025

Background and Objectives: Oxygen is essential for all living organisms and plays a critical role in anesthesia and intensive care practices. However, the notion that unlimited oxygen therapy is harmless is a misconception. Our study investigates the acute effects of different preoxygenation methods on hemodynamic parameters and neurodegenerative biomarkers in patients undergoing laparoscopic cholecystectomy surgery. Materials and Methods: This prospective, randomized, controlled study included 52 patients undergoing elective laparoscopic cholecystectomy under general anesthesia. Patients were divided into two groups: Group I received standard preoxygenation (100% FiO2 for 3 min), while Group II underwent rapid preoxygenation (eight deep breaths over 30 s to 1 min). Hemodynamic parameters (SAP, DAP, MAP, and SpO2) and neurodegenerative biomarkers (pTau, S100B, NSE, NfL, GFAP) were measured after preoxygenation, after intubation, and at the end of surgery. Results: Group I exhibited a significant increase in levels of pTau, S100B, NSE, and GFAP, indicating higher neuronal and glial cell stress compared to Group II (p < 0.001). No significant increase in NfL levels was observed in either group. Hemodynamic parameters (HR, SAP, DAP, MAP) were significantly higher during and after preoxygenation in Group I, suggesting an increased stress response. Group II showed lower levels of acute neurotoxicity and oxidative stress. Conclusions: Our findings indicate that preoxygenation with 100% FiO2 induces stress in neuronal cells, axons, and glial cells, leading to an increase in neurodegenerative biomarkers. Optimizing preoxygenation strategies is crucial to reduce oxidative stress and improve neurological outcomes for surgical patients.
Hybrid Solder Joints: Viscosity Studies of the Nanocomposite Flux with Fe Nanoparticle Additions
Authors: Yakymovych Andriy , Wodak Irina , Khatibi Golta
Published: January 2025

Viscosity is one of the most important physical characteristics of choosing a flux for solder paste. However, not in all cases do the producers give information about the viscosity of commercial flux. A recent promising trend is to mix various kinds of nanoparticles with solder paste or flux and investigate the produced solder joints. However, the impact of nanosized inclusions on the viscosity of the flux has practically not been investigated. In this study, the temperature and shear rate dependencies of the viscosity of the nanocomposite flux were measured. For this purpose, the commercial flux has been mixed with Fe nanoparticles up to 2.0 wt.% of inclusions. It has been shown that viscosity increases with the addition of Fe nanoparticles. However, it is valid only for first heating up to circa 340 K. The viscosity values of the flux with and without nanosized inclusions are practically similar by further heating to 360 K as well as by subsequent cooling. Additionally, the performed differential thermal analysis has shown the heat effects, which are in good agreement with viscosity behavior.
Enhanced Cutting Performance of 50Cr15MoV Martensitic Stainless Steel Through Controlled Residual Austenite Stability
Authors: Guo Fujian , Peng Zhimin , Lu Guangyi , Liu Wenle , Li Guoqing , Zhang Pan , Shang Chengjia
Published: January 2025

The relationship between the stability of tool materials and their cutting performance is a critical technical challenge for the manufacturing industry, which is essential for selecting appropriate treatment processes to achieve superior treatment tool performance. In this paper, a standard cutting tool experiment was used to study the sharpness of the knife with different residual austenite content. The cutting edges of the knife were characterized by an optical microscope (OM), scanning electron microscope (SEM), electron back scattering diffraction (EBSD), and transmission electron microscope (TEM), to analyze the relationship between tool edge hardness and microstructure. The microstructure stability of the material was analyzed by a separated Hopkinson pressure bar (SHPB) experiment. The results show that the hardness and cutting performance of the knives are affected by the joint action of carbide and residual austenite, with an initial increase followed by decreases as the heat treatment quenching temperature increases. After the knife material is treated by cryogenic process, the hardness of the knife is increased by 3.89 HRC, the initial sharpness by 15.3%, and the sharpness and durability by 18.8%. The residual austenite in the knives was found to be unstable and easy to transformation during high-rate deformation processes. This study elucidates the effect of residual austenite content on the sharpness of the knives, providing a foundation for the reasonable control of residual austenite content in the actual production settings.
Enhanced Performance of Novel Amorphous Silicon Carbide Microelectrode Arrays in Rat Motor Cortex
Authors: Haghighi Pegah , Jeakle Eleanor N. , Sturgill Brandon S. , Abbott Justin R. , Solis Elysandra , Devata Veda S. , Vijayakumar Gayathri , Hernandez-Reynoso Ana G. , Cogan Stuart F. , Pancrazio Joseph J.
Published: January 2025

Implantable microelectrode arrays (MEAs) enable the recording of electrical activity from cortical neurons for applications that include brain–machine interfaces. However, MEAs show reduced recording capabilities under chronic implantation conditions. This has largely been attributed to the brain’s foreign body response, which is marked by neuroinflammation and gliosis in the immediate vicinity of the MEA implantation site. This has prompted the development of novel MEAs with either coatings or architectures that aim to reduce the tissue response. The present study examines the comparative performance of multi-shank planar, silicon-based devices and low-flexural-rigidity amorphous silicon carbide (a-SiC) MEAs that have a similar architecture but differ with respect to the shank cross-sectional area. Data from a-SiC arrays were previously reported in a prior study from our group. In a manner consistent with the prior work, larger cross-sectional area silicon-based arrays were implanted in the motor cortex of female Sprague-Dawley rats and weekly recordings were made for 16 weeks after implantation. Single unit metrics from the recordings were compared over the implantation period between the device types. Overall, the expression of single units measured from a-SiC devices was significantly higher than for silicon-based MEAs throughout the implantation period. Immunohistochemical analysis demonstrated reduced neuroinflammation and gliosis around the a-SiC MEAs compared to silicon-based devices. Our findings demonstrate that the a-SiC MEAs with a smaller shank cross-sectional area can record single unit activity with more stability and exhibit a reduced inflammatory response compared to the silicon-based device employed in this study.
A Compact Device Model for a Piezoelectric Nano-Transistor
Authors: McCartney L. Neil , Crocker Louise E. , Wright Louise , Rungger Ivan
Published: January 2025

An approximate compact model was developed to provide a convenient method of exploring the initial design space when investigating the performance of micro-electronic devices such as nano-scaled piezoelectronic transistors, where fast ball-park estimates can be very helpful. First of all, the compact model was verified by comparing its predictions with those of accurate axi-symmetric finite element analysis (FEA) using special boundary and interface conditions that enable the replication of the analytical model behaviour. Verification is achieved for a radio frequency (RF) switch and a smaller very-large-scale integrated (VLSI) device, where percentage differences between the compact and FEA model predictions are of the order 10−4 for the RF switch and 10−5 for the VLSI device. This confirms the consistency of complex property data (especially electro-thermo-elastic constants) and geometrical parameter input to both types of models and convincingly demonstrates that the analytical models and FEA for the two devices have been implemented correctly. A second type of boundary and interface condition is also used that is designed to replicate the actual behaviour of the devices in practice. The boundary and interface constraints applied for the verification procedure are relaxed so that there is perfect interface bonding between layers. For this unconstrained case, the resulting deformation is very complex, involving both bending effects and edge effects arising from property mismatches between neighbouring layers. The results for the RF switch show surprisingly good agreement between the predictions of the analytical and FEA results, provided the thickness of the piezoelectric layer is not too thick, implying that the analytical model should help to reduce the parameter design space for such devices. However, for the VLSI device, our results indicate that the compact model leads to much larger errors. For such systems, the compact model is unlikely to be able to reliably reduce the parameter design space, implying that accurate FEA will then need to be used.
Analysis of Bacterial and Fungal Communities and Organic Acid Content in New Zealand Lambic-Style Beers: A Climatic and Global Perspective
Authors: Ohwofasa Aghogho , Dhami Manpreet , Winefield Christopher , On Stephen L. W.
Published: January 2025

Beer produced by autochthonous microbial fermentation is a long-established craft beer style in Belgium that has now been implemented commercially in New Zealand. We used a metabarcoding approach to characterize the microbiome of 11 spontaneously fermented beers produced by a single brewery in Oamaru from 2016 to 2022. Key organic acid concentrations were also determined. Both bacterial and fungal populations varied considerably between vintages and between individual brews produced in 2020. Similarly, for organic acids, the concentrations of L-malic acid, succinic acid, and L-lactic acid statistically differed from one vintage to another. Moreover, a correlation between the concentrations of certain organic acids and microbial composition was inferred by ordination analyses. Through reference to publicly available climate data, humidity and maximum temperature seemed to enhance the abundance of Penicillium and Hanseniaspora in beer microbiota. However, comparison with previously published studies of Belgian lambic beers, similar Russian ales, and publicly available temperature data from these regions showed that the microbial populations of these were relatively stable despite greater extremes of weather. Our results suggest that while climatic variables may influence microbial populations during beer making that employs autochthonous fermentation in New Zealand, such variation is not evident where similar beers are produced in facilities with a long-established history of production. These findings have implications for lambic-style beer production in the context of global climate change, notably where microbial populations may lack environmental adaptation.
Engineering Inorganic Pyrophosphate Metabolism as a Strategy to Generate a Fluoride-Resistant Saccharomyces cerevisiae Strain
Authors: Perez-Castiñeira José R. , Ávila-Oliva Francisco J. , Serrano Aurelio
Published: January 2025

Fluorine accounts for 0.3 g/kg of the Earth’s crust, being widely distributed in the environment as fluoride. The toxic effects of this anion in humans and other organisms have been known for a long time. Fluoride has been reported to alter several cellular processes although the mechanisms involved are largely unknown. Inorganic pyrophosphatases (PPases) are ubiquitous enzymes that hydrolyze inorganic pyrophosphate (PPi), a metabolite generated from ATP. In Saccharomyces cerevisiae, the enzyme responsible for PPi hydrolysis in the cytosol (IPP1) is strongly inhibited by fluoride in vitro. The essentiality of IPP1 for growth has been previously demonstrated using YPC3, a yeast mutant with conditional expression of the corresponding gene. Here, YPC3 was used to generate cells that tolerate high concentrations of fluoride by (a) the overexpression of IPP1 or its human ortholog, or (b) the substitution of IPP1 by the fluoride-insensitive PPase from Streptococcus mutans. The results obtained suggest that maintaining appropriate levels of PPase activity in the cytosol is essential for the adaptation of S. cerevisiae to high fluoride concentrations. The increase in fluoride tolerance allows YPC3 cells transformed with suitable plasmids to be selected on rich non-selective medium supplemented with this anion.
The HELP-UnaG Fusion Protein as a Bilirubin Biosensor: From Theory to Mature Technological Development
Authors: Sist Paola , Urbani Ranieri , Tramer Federica , Bandiera Antonella , Passamonti Sabina
Published: January 2025

HUG is the HELP-UnaG recombinant fusion protein featuring the typical functions of both HELP and UnaG. In HUG, the HELP domain is a thermoresponsive human elastin-like polypeptide. It forms a shield enwrapping the UnaG domain that emits bilirubin-dependent fluorescence. Here, we recapitulate the technological development of this bifunctional synthetic protein from the theoretical background of its distinct protein moieties to the detailed characterization of its macromolecular and functional properties. These pieces of knowledge are the foundations for HUG production and application in the fluorometric analysis of bilirubin and its congeners, biliverdin and bilirubin glucuronide. These bile pigments are metabolites that arise from the catabolism of heme, the prosthetic group of cytochromes, hemoglobin and several other intracellular enzymes engaged in electron transfer, oxygen transport and protection against oxygen free radicals. The HUG assay is a powerful, user-friendly and affordable analytical tool that alone supports research at each level of complexity or taxonomy of living entities, from enzymology, cell biology and pathophysiology to veterinary and clinical sciences.
New Compounds with Enhanced Biological Activity Through the Strategic Introduction of Silylated Groups into Hydroxystearic Acids
Authors: Zalambani Chiara , Anconelli Lorenzo , Calonghi Natalia , Telese Dario , Micheletti Gabriele , Boga Carla , Farruggia Giovanna , Pagnotta Eleonora
Published: January 2025

In the field of medicinal chemistry, the introduction of silylated groups is an important strategy to alter the activity, selectivity, and pharmacokinetics of compounds based on the diverse traits of silicon, including atomic size, electronegativity, and hydrophobicity. The hydroxy group on C-9 or C-9 and C-10 of hydroxystearic acids have been functionalized as t-butyl dimethyl silyl ether. The target compounds have been fully characterized and tested for in vitro cytotoxicity in tumor cells HT29, HCT116, CaCo2, HeLa, MCF7, U2OS, and Jurkat J6 and normal I407 cells. In particular, the silyl derivative of (R)-9-hydroxystearic acid was more active in colon cancer cells. Analyses of cell proliferation, oxidative cell status, histones post-translational modifications, protein phosphorylation, gene expression, and DNA damage were performed to obtain information on the antitumor properties of the new molecules in comparison with the unmodified (R)-9-hydroxystearic acid’s previously studied effects. Our results suggest that the incorporation of a silyl functionality may be a useful tool for the structural development of new pharmaceutically active compounds against colon cancer.
Nuclear Quantum Effects in the Ionic Dissociation Dynamics of HCl on the Water Ice Cluster
Authors: Murakami Tatsuhiro , Ueno Hinami , Kikuma Yuya , Takayanagi Toshiyuki
Published: January 2025

Nuclear quantum effects play a significant role in the dissociation dynamics of HCl ions during collisions with the (H2O)49 ice cluster. These effects become particularly important when analyzing proton transfer, tunneling, and zero-point energy contributions during the dissociation process. In this study, we investigate the dissociation behavior of HCl when colliding with the (H2O)49 ice cluster, focusing on the influence of the nuclear quantum effects on the proton transfer mechanism, ionic dissociation rates, and subsequent solvation dynamics. Through a combination of classical molecular dynamics (MD) and ring-polymer molecular dynamics (RPMD) simulations, we explore how quantum fluctuations in the proton’s position alter the dissociation pathway of HCl. The inclusion of nuclear quantum effects reveals enhanced proton mobility, leading to differences in dissociation behavior compared to classical simulations. Our findings indicate that nuclear quantum effects significantly affect the dissociation dynamics, with the proton more readily transferring to the hydrogen-bond network in the (H2O)49 ice cluster. This study provides insights into the quantum mechanical nature of ionic dissociation in hydrogen-bonded systems and highlights the importance of incorporating nuclear quantum effects for accurate modeling of proton transfer processes in complex environments.
Curcumin in Ophthalmology: Mechanisms, Challenges, and Emerging Opportunities
Authors: Ribeiro Adriana , Oliveira Daniele , Cabral-Marques Helena
Published: January 2025

Ocular diseases affecting the anterior and posterior segments of the eye are major causes of global vision impairment. Curcumin, a natural polyphenol, exhibits anti-inflammatory, antioxidant, antibacterial, and neuroprotective properties, making it a promising candidate for ocular therapy. However, its clinical use is hindered by low aqueous solubility, poor bioavailability, and rapid systemic elimination. This review comprehensively highlights advances in curcumin delivery systems aimed at overcoming these challenges. Emerging platforms, including proniosomal gels, transferosomes, and cyclodextrin complexes, have improved solubility, permeability, and ocular retention. Nanoparticle-based carriers, such as hybrid hydrogels and biodegradable nanoparticles, enable sustained release and targeted delivery, supporting treatments for posterior segment diseases like diabetic retinopathy and age-related macular degeneration. For anterior segment conditions, including keratitis and dry eye syndrome, cyclodextrin-based complexes and mucoadhesive systems enhance corneal permeability and drug retention. Mechanistically, curcumin modulates key pathways, such as NF-κB and TLR4, reducing oxidative stress, angiogenesis, and apoptosis. Emerging strategies like photodynamic therapy and neuroprotective approaches broaden their application to eyelid conditions and neuroinflammatory ocular diseases. These advancements address curcumin’s pharmacokinetic limitations, supporting its clinical translation into ophthalmic therapies. This work underscores curcumin’s potential in ocular disease management and advocates clinical trials to validate its safety, efficacy, and therapeutic relevance.
An Efficient Synthesis of 3,5-Bis-Aminated Pyrazolo[1,5-a]Pyrimidines: Microwave-Assisted Copper Catalyzed C-3 Amination of 5-Amino-3-Bromo-Substituted Precursors
Authors: Iorkula Terungwa H. , Tolman Bryce A. , Ganiyu Latifat O. , Peterson Matt A.
Published: January 2025

An efficient method has been developed for the rapid production of diverse arrays of 3,5-bis-aminated pyrazolo[1,5-a]pyrimidines. The method utilizes CuI (5 mol%) and carbazole-based ligand L-1 (N-(9H-carbazol-9-yl)-1H-pyrrole-2-carboxamide) (10 mol%) for efficient Ullmann-type coupling of various amines to 5-amino-3-bromopyrazolo[1,5-a]pyrimidine precursors after heating in diethylene glycol (DEG) for only 1 h at 80 °C (microwave heating). 3,5-Bis-aminated products were obtained in good to excellent yields (60–93%, 83% average for 29 examples). 1° and 2° alkylamines, as well as a variety of aryl- or heteroarylamines coupled efficiently, and 1° and 2° alkyl (or aryl) amines at C-5 were well tolerated. The optimized conditions worked well on both the 50 mg and 1.0 g scales and gave products in only two steps from commercially available 3-bromo-5-chloropyrazolo[1,5-a]pyrimidine. Advantages provided by this method include short reaction time, excellent yields, broad substrate scope, and avoidance of toxic reagents commonly utilized for reductive aminations of C-3 NH2 substituted precursors.
Human Pharmacokinetic Profiling and Comparative Analysis of Mangiferin and Its Monosodium Derivative from Mangifera indica Extracts Using UHPLC-MS/MS with 1H NMR and MALDI-TOF Confirmation
Authors: Fuentes-Rios David , Sanchez-Rodriguez Alvaro , Lopez-Rios Laura , Garcia-Gonzalez Eduardo , Martinez-Canton Miriam , Galvan-Alvarez Victor , Gallego-Selles Angel , Martin-Rincon Marcos , Calbet Jose A. L. , Vega-Morales Tanausu
Published: January 2025

Mangiferin, a glucosyl xanthone, is a plant metabolite with promising nootropic and ergogenic properties. However, its poor and inconsistent systemic bioavailability limits its therapeutic potential. Additionally, the pharmacokinetics of mangiferin from mango leaf extract formulations remain uncharacterized in humans. This study validated a UHPLC-MS/MS method and conducted a human pharmacokinetic study approved by an ethics committee. The bioavailability of mangiferin and its monosodium salt was assessed from two standardized mango leaf extracts: MLE60, standardized to 60% mangiferin but practically insoluble in water, and MLES, the water-soluble monosodium salt form, also standardized to 60%. Twelve participants (six females) received oral doses of each extract in a crossover design with a 7-day washout period. Plasma analysis showed significantly higher AUC and Cmax values with MLES than MLE60, while Tmax and T1/2 were similar. MLES demonstrated a 2.44-fold increase in AUC0–24h compared to MLE60 (p = 0.0029 **), indicating improved bioavailability. This study highlights the salification method as a simple strategy to enhance mangiferin bioavailability, enabling broader applications in beverages and other products where solubility is a limitation.
fMRI Insights into Visual Cortex Dysfunction as a Biomarker for Migraine with Aura
Authors: Pikor Damian , Banaszek-Hurla Natalia , Drelichowska Alicja , Hurla Mikołaj , Dorszewska Jolanta , Wolak Tomasz , Kozubski Wojciech
Published: January 2025

Migraine with aura (MwA) is a common and severely disabling neurological disorder, characterised by transient yet recurrent visual disturbances, including scintillating scotomas, flickering photopsias, and complex geometric patterns. These episodic visual phenomena significantly compromise daily functioning, productivity, and overall quality of life. Despite extensive research, the underlying pathophysiological mechanisms remain only partially understood. Cortical spreading depression (CSD), a propagating wave of neuronal and glial depolarisation, has been identified as a central process in MwA. This phenomenon is triggered by ion channel dysfunction, leading to elevated intracellular calcium levels and excessive glutamate release, which contribute to widespread cortical hyperexcitability. Genetic studies, particularly involving the CACNA gene family, further implicate dysregulation of calcium channels in the pathogenesis of MwA. Recent advances in neuroimaging, particularly functional magnetic resonance imaging (fMRI), have provided critical insights into the neurophysiology of MwA. These results support the central role of CSD as a basic mechanism behind MwA and imply that cortical dysfunction endures beyond brief episodes, possibly due to chronic neuronal dysregulation or hyperexcitability. The visual cortex of MwA patients exhibits activation patterns in comparison to other neuroimaging studies, supporting the possibility that it is a disease-specific biomarker. Its distinctive sensory and cognitive characteristics are influenced by a complex interplay of cortical, vascular, and genetic factors, demonstrating the multifactorial nature of MwA. We now know much more about the pathophysiology of MwA thanks to the combination of molecular and genetic research with sophisticated neuroimaging techniques like arterial spin labelling (ASL) and fMRI. This review aims to synthesize current knowledge and analyse molecular and neurophysiological targets, providing a foundation for developing targeted therapies to modulate cortical excitability, restore neural network stability, and alleviate the burden of migraine with aura. The most important and impactful research in our field has been the focus of this review, which highlights important developments and their contributions to the knowledge and treatment of migraine with aura.
Maternal Infections, Antibiotics, Steroid Use, and Diabetes Mellitus Increase Risk of Early-Onset Sepsis in Preterm Neonates: A Nationwide Population-Based Study
Authors: Lee Hao-Yuan , Hsu Yu-Lung , Lee Wen-Yuan , Huang Kuang-Hua , Tsai Ming-Luen , Chen Chyi-Liang , Chang Yu-Chia , Lin Hung-Chih
Published: January 2025

The global evolution of pathogens causing early-onset sepsis (EOS), a critical condition in preterm infants, necessitates a re-evaluation of risk factors to develop updated prevention and treatment strategies. This nationwide case–control study in Taiwan analyzed data from the National Health Insurance Research Database, Birth Reporting Database, and Maternal and Child Health Database from 2010 to 2019. The study included 176,681 mother–child pairs with preterm births. We identified 2942 clinical EOS cases from 5535 diagnosed sepsis cases, excluding unlikely cases. A control group of 14,710 preterm neonates without EOS was selected at a 1:5 ratio. Clinical EOS increased since 2017. Adjusted logistic regression identified significant EOS risk factors in preterm infants, including maternal fever, chorioamnionitis, maternal diabetes mellitus, maternal antibiotic usage, very preterm birth, birth weight (all with p < 0.001), maternal pneumonia (p = 0.002), and maternal CS (p = 0.004). Effective treatment of maternal conditions like diabetes, fever, and infections is essential to prevent EOS in preterm infants. Key measures include reducing unnecessary antibiotics or steroids, minimizing unnecessary cesarean sections, avoiding premature or prolonged rupture of membranes (PPROM), and increasing gestational age and neonatal birth weight. High-risk preterm neonates should be closely monitored for EOS and considered for antibiotics when warranted.
Research Progress in In Vitro Screening Techniques for Natural Antithrombotic Medicines
Authors: Liu Xinyang , Chen Lu , Li Lin , Yan Yiqi , Zhang Han
Published: January 2025

Natural medicines play an indispensable role in treating thrombotic-related diseases and a thorough investigation of their material basis is crucial for medicine development. The rapid advancement in medicine-active component screening technologies has paved new avenues for studying natural medicines, holding significant theoretical and practical value. This review focuses on the application progress of multimodal screening technologies, including high-throughput screening, chip technology, molecular biology methods, fluorescence sensors, and computational biology, in the screening of anticoagulant medicines. The aim is to provide a reference framework for screening and validating active components in natural medicines. The early application of these technologies can swiftly assess the safety and efficacy of medicines, accelerating the medicine development process and reducing the failure rate in clinical trials. Nonetheless, the overall mechanisms of action of natural medicines and the correlation between their chemical components and thrombotic diseases remain challenging areas that require further in-depth exploration and technological innovation.
Losses and Energy Backflows of the Fundamental Core Mode in Solid Core Micro Structured Optical Fibers
Authors: Pryamikov Andrey
Published: January 2025

This work discusses the behavior of transverse energy fluxes of the fundamental core mode of a holey fibers and a photonic band gap fibers when the polarization state of this mode changes. The behavior of the transverse component of the Poynting vector of the fundamental core mode is considered for both linear and elliptical polarization. It is demonstrated that despite the difference in the distribution of the Poynting vector stream lines in the cross section of the fibers for the two polarizations, the leakage loss level is maintained constant due to the forward and reverse energy flows in the radial direction. Differences in the level of leakage losses in different micro structured fibers arise from the vortex structure of the Poynting vector of the fundamental core mode.
A Detailed Study of a Resonant-Assisted Segmented Cladding Fiber for Large Mode Area Applications
Authors: Xue Minmin , Qin Hao , Li Suwen , Hao Yuqi , Yuan Libo
Published: January 2025

In this work, we have proposed and optimized a large mode area segmented cladding fiber (SCF) with an unconventional low-index segment cladding. The low-refractive-index cladding proposed in this paper consists of three parts. There three geometric parameters chosen as design variables were the length and width of the first part and the leg length of the isosceles trapezoid in the second part. To investigate the properties of the proposed SCF, numerical modeling based on the finite element method (FEM) was performed. A high leakage loss ratio (>9000) between the high-order modes (HOMs) and the fundamental mode was achieved at a wavelength of 1310 nm, which is significantly higher than that of conventional SCFs. Additionally, the mode area of the proposed fiber reaches 890 µm2 at a core radius of 20 µm. The bending effects on the SCF were also studied. When the bending radius is greater than 0.3 m, the mode area greater than 880 µm2 and remains stable, and the leakage loss of the least high-order mode (LP11h) exceeds 30 dB/m. The new fiber design demonstrates significant potential for high-power fiber lasers
The Vascular Flora of Pisa (Tuscany, Central Italy)
Authors: Peruzzi Lorenzo , Pierini Brunello , Arduini Iduna , Bedini Gianni , Franzoni Jacopo
Published: January 2025

We present the first vascular flora of the municipality of Pisa. The floristic inventory was built on previous literature and field observations deposited in the online database Wikiplantbase #Toscana, integrated by observations from iNaturalist. The established flora of Pisa includes a total of 1404 specific and subspecific taxa (594 genera, 123 families), out of which 112 are alien species. Silene subconica is excluded from the regional flora of Tuscany, while Solanum nitidibaccatum is reported as a new regional casual alien, and the regional alien status of Salpichroa origanifolia shifts from naturalized to invasive. Native taxa exceed species-area predictions by 33.3%, attesting for a high floristic richness, and there are taxa of high biogeographical and conservation interest. However, also alien taxa exceed predictions by 34.9%, and there are many invasive species, pointing out a high anthropogenic impact in the territory of Pisa, mostly due to urbanization. The biological and chorological spectra reflect the coexistence of typical Mediterranean and central European habitats in this territory, especially within the Migliarino–San Rossore–Massaciuccoli Regional Park. The vascular flora of the municipality is quite rich, although threatened by anthropic pressures, fostering the arrival and establishment of invasive alien species.
Expression Analysis of Chlorophyll-Degradation-Related Genes in Prunus persica L. Peel and the Functional Verification of Key Genes
Authors: Liu Xin , Zhang Xiaoyu , Meng Junren , Li Ang , Duan Wenyi , Sun Shihang , Pan Lei , Zeng Wenfang , Wang Zhiqiang , Niu Liang
Published: January 2025

With the evolution of consumer purchasing power and consumption concepts, external attributes such as fruit size, color, and peel smoothness have emerged as pivotal determinants influencing purchasing preferences; among these, the background color of the fruit peel exerts a considerable impact on fruit esthetics. The background color of fruit peel is predominantly influenced by the chlorophyll content. Consequently, examining the degradation patterns of chlorophyll in Prunus persica L. peel holds significant importance for cultivating varieties with a cleaner peel background color. In this study, Prunus persica L. CP14 and 20–29 were selected as experimental materials to evaluate the peel color variation and chlorophyll content during fruit development. Samples collected from three developmental stages of CP14 and 20–29 underwent transcriptome sequencing. Kyoto Encyclopedia of Genes and Genomes enrichment analysis identified chlorophyll-degradation-related genes within the purine metabolism pathway. Quantitative polymerase chain reaction analysis of chlorophyll degradation gene expression pinpointed PpPAO and PpSGR as likely key genes involved in chlorophyll degradation in Prunus persica L. Transient transformation assays in Nicotiana benthamiana leaves further substantiated that PpPAO and PpSGR markedly reduce chlorophyll levels. Yeast two-hybrid experiments also demonstrated an interaction between PpPAO and PpSGR.
Characterization and Adsorption Behavior of Newly Synthesized Aminated Cellulose with Jeffamine EDR148 Towards Ni(II), Cu(II), and Pb(II) Heavy Metal Ions
Authors: Al Nawah Jawaher Y. , El-Khouly Amany S.
Published: January 2025

Industrial wastewater containing heavy metal ions presents serious economic risk to the environment. In this study, a novel compound of aminated cellulose with jeffamine EDR148 was prepared to improve cellulose’s adsorptive behavior towards metal ions. This study undertook a straightforward and efficient cellulose modification through homogeneous chlorination in N,N′-butylmethylimidazolium chloride to produce 6-deoxychlorocellulose (Cell-Cl), followed by a reaction with jeffamine EDR148 and ultimately resulting in the formation of aminated cellulose (Cell-Jef148). Structural and chemical characteristics of Cell-Cl and Cell-Jef148 were determined using different techniques. Various adsorption conditions were applied to evaluate the optimal adsorption conditions for the removal of Cu(II), Ni(II), and Pb(II) ions. Cell-Jef48 revealed a greater affinity and higher adsorption efficiency of 480.3, 420.5, and 463.2 mg/g for Cu(II), Ni(II), and Pb(II) ions, respectively. Different kinetics and adsorption isothermal models were studied to investigate the adsorption mechanism and interactions between Cell-Jef148 and metal ions. The results fitted the Langmuir and pseudo-second-order models. Corresponding to the Langmuir model, Cell-Jef148’s maximum adsorption capacities were 952.38, 609.76, and 769.23 mg/g for Cu(II), Ni(II), and Pb(II) ions, respectively, with a high correlation coefficient, R2, in the range of 0.99575–0.99855. The research results of this study support Cell-Jef148’s adsorption of heavy metal ions, and the regeneration of adsorbent highlights the potential applications of cellulose-based materials in wastewater treatment.
Mineral Filler Hybridization in Recycled Polyethylene Terephthalate
Authors: Droß Marcel , Ehleben Max , Dröder Klaus
Published: January 2025

This study focused on evaluating the mechanical, thermal, and morphological properties of recycled polyethylene terephthalate (RPET) hybrid mineral-filled composites containing fine acicular wollastonite, mica phlogopite, and talc platelets. Depending on the filler content, both single mineral-filled composites as well as hybrid mineral–filler composites were investigated. The maximum nominal filler content was set to 20% by weight with varying ratios for combinations of the wollastonite–mica and wollastonite–talc composites, respectively. Aside from the tensile, compression, and flexural properties, the heat distortion temperature and degree of crystallinity were carried out. Moreover, the dynamical response of the hybrid mineral-filled composites on different frequencies (1 Hz, 2 Hz, 5 Hz, and 10 Hz) was considered. By using scanning electron microscope photography, the fracture surface and the morphology of the composite material were observed. The results demonstrated enhanced stiffness, strengths, and thermal stability for all hybrid mineral-filled composites. In particular, the wollastonite–talc-filled RPET composites revealed a good compatibility and showed the most beneficial results.
Improvement in the Damping Behavior of Hierarchical Carbon Fiber-Reinforced Plastic for Park Golf Club Faces
Authors: Bae Seoyeon , Shin Minhyeok , Kim Eunjung , Lee Sungbi , Yu Woong-Ryeol , Ahn Cheol-Hee , Na Wonjin
Published: January 2025

Park golf, introduced to Korea in 2000, has become a popular leisure activity, especially among older people. However, sudden shock between the ball and carbon fiber-reinforced plastic (CFRP) face can increase the risk of injuries, highlighting the need for enhanced damping material. However, restitution and damping are critical properties of golf clubs and often exhibit a conflicting relationship; thus, a method is needed to address this challenge. Therefore, this study aimed to develop a CFRP with an enhanced restitution and damping ratio by incorporating carbon nanotubes and graphene oxide nanofillers into the existing CFRP face material. A drop test apparatus was set up to measure the coefficient of restitution, and the damping properties were evaluated using a pencil lead-breaking test. CNTs exhibited high rebound properties due to their stiffness and hardness. In contrast, GO provided a modest increase in rebound while demonstrating a superior damping ratio, attributed to its layered structure and high internal friction. Based on these results, the optimal nanofiller content was determined as GO 0.025 wt%, showing a minor improvement in rebound performance, a 1033% improvement in vibration damping, and an 84% improvement in acoustic damping. Notably, this finding implies the importance of nanomaterial shape and its interaction with the composite matrix. A double-masked user test with a prototype confirmed enhanced comfort and reduced vibration feedback. The low-vibration components developed in this study are expected to be applicable in future research for controlling the damping ratio under impact or vibrations, such as UAM and helicopters.
Laboratory Evaluation of Asphalt Binder and Asphalt Mixture Modified Using Styrene–Butadiene–Styrene/Rock Asphalt
Authors: Liao Hongbo , Yang Xin , Yan Jing , Du Xiaobo , Lin Hongwei
Published: January 2025

This study investigates the performance enhancement of asphalt and its mixtures through modification with varying contents of styrene–butadiene–styrene (SBS) and rock asphalt (RA). A series of laboratory tests were conducted to comprehensively evaluate the effects of SBS and RA modification. The results demonstrated that SBS significantly improves elasticity, low-temperature ductility, and resistance to fatigue and rutting, while RA enhances high-temperature stability, water stability, and stripping resistance. The synergistic effects of SBS and RA were evident, with the 4% SBS/10% RA mixture achieving the best comprehensive performance, which was characterized by improved high-temperature stability, low-temperature performance, and durability. Conversely, 15% RA content was found to reduce low-temperature flexibility and fatigue performance. A radar chart-based evaluation further confirmed the optimal performance of the 4% SBS/10% RA combination. These findings provide valuable guidance for selecting appropriate SBS and RA proportions tailored to specific performance requirements in asphalt pavement applications.
Toward Efficient Edge Detection: A Novel Optimization Method Based on Integral Image Technology and Canny Edge Detection
Authors: Li Yanqin , Zhang Dehai
Published: January 2025

The traditional SIFT (Scale Invariant Feature Transform) registration algorithm is highly regarded in the field of image processing due to its scale invariance, rotation invariance, and robustness to noise. However, it faces challenges such as a large number of feature points, high computational demand, and poor real-time performance when dealing with large-scale images. A novel optimization method based on integral image technology and canny edge detection is presented in this paper, aiming to maintain the core advantages of the SIFT algorithm while reducing the complexity involved in image registration computations, enhancing the efficiency of the algorithm for real-time image processing, and better adaption to the needs of large-scale image handling. Firstly, Gaussian separation techniques were used to simplify Gaussian filtering, followed by the application of integral image techniques to accelerate the construction of the entire pyramid. Additionally, during the feature point detection phase, an innovative feature point filtering strategy was introduced by combining Canny edge detection with dilation operations alongside the traditional SIFT approach, aiming to reduce the number of feature points and thereby lessen the computational load. The method proposed in this paper takes 0.0134 s for Image type a, 0.0504 s for Image type b, and 0.0212 s for Image type c. In contrast, the traditional method takes 0.1452 s for Image type a, 0.5276 s for Image type b, and 0.2717 s for Image type c, resulting in reductions of 0.1318 s, 0.4772 s, and 0.2505 s, respectively. A series of comparative experiments showed that the time taken to construct the Gaussian pyramid using our proposed method was consistently lower than that required by the traditional method, indicating greater efficiency and stability regardless of image size or type.
Simulation Tool for the Techno-Economic Assessment of the Integrated Production of Polyhydroxyalkanoates as Value-Added Byproducts of a Wastewater Treatment Plant
Authors: Pozo-Morales Laura , Rosales Martínez Antonio , Baquerizo Enrique , del Valle Agulla Germán
Published: January 2025

The polyhydroxyalkanoate (PHA) production process that uses mixed microbial cultures combined with main stream wastewater treatment plants (WWTPs) is a competitive integrated resource recovery process in which non-oxygen electron acceptors can be used to enrich the PHA producer. Trials carried out in operating plants are very scarce, and there are no simulation tools available to analyse the feasibility of integrating the two processes. This research presents a novel analysis tool for a techno-economic assessment of value-added biopolymers. A general model for a conventional WWTP has been designed and eventually validated using the operating data collected in the database of a fully operational plant. In the model, a simulation of a PHA production line based on thickened primary sludge as a substrate has been integrated. The assembly has been treated as a closed-loop system with an accuracy level of 0.1% with a limit of 1000 iterations. Two strategies based on internal (ADF) or external (AN/AD) limitations of some nutrients have been contrasted for the selection of a biomass capable of feast–famine PHA synthesis. The ADF strategy was found to be the most favourable system, with a production of 0.226 kg of CODPHA·kg−1 COD. The calculated production cost was EUR 0.11·kg−1 CODPHA. The sludge production was reduced by 6%.
Religiously Grounded Character and Its Association with Subjective Well-Being in Emerging Adults: A Latent Profile Analysis
Authors: Villani Daniela , Eissa Sara , Zambelli Michela , Di Natale Anna Flavia
Published: January 2025

Subjective Well-Being (SWB) is a central indicator of mental health and overall quality of life in emerging adulthood; religiosity can contribute to this outcome. Emerging research suggests that religious identity and virtues not only can contribute independently to SWB but may work together in shaping it. Given this interplay, the present study adopts a person-centered approach, using Latent Profile Analysis (LPA), to investigate profiles of emerging adults showing a similar configuration of religious identity and virtues and to examine their contribution to SWB. Two-hundred and ninety-one emerging adults living in Italy aged 18 to 30 completed an online survey asking for various demographic variables, religious status, religious affiliation, religious practices, religious identity (U-MICS Religious domain), character strengths and virtues (VIA- IS), and subjective well-being (SWLS and SPANE). A best-fitting model consisting of three distinct, non-overlapping profiles, each demonstrating a unique configuration of religious identity dimensions (commitment, in-depth exploration, reconsideration) and virtues (Transcendence, Humanity, Temperance), emerged. These profiles were differently associated with SWB. Specifically, individuals in the Engaged with High Religious Virtues profile displayed high levels of religious commitment and exploration, paired with high levels of religious virtues, particularly Transcendence, and exhibited the highest levels of SWB. These findings suggest that religious identity and virtues are strictly intertwined and that higher levels of this conjoint integration correspond to greater SWB. Future interventions aimed at fostering religious self-exploration and cultivating religiously grounded virtues can be particularly effective at this stage of the life cycle.
Modeling the Inter-Arrival Time Between Severe Storms in the United States Using Finite Mixtures
Authors: Vinnik Ilana , Miljkovic Tatjana
Published: January 2025

When inter-arrival times between events follow an exponential distribution, this implies a Poisson frequency of events, as both models assume events occur independently and at a constant average rate. However, these assumptions are often violated in real-insurance applications. When the rate at which events occur changes over time, the exponential distribution becomes unsuitable. In this paper, we study the distribution of inter-arrival times of severe storms, which exhibit substantial variability, violating the assumption of a constant average rate. A new approach is proposed for modeling severe storm recurrence patterns using a finite mixture of log-normal distributions. This approach effectively captures both frequent, closely spaced storm events and extended quiet periods, addressing the inherent variability in inter-event durations. Parameter estimation is performed using the Expectation–Maximization algorithm, with model selection validated via the Bayesian information criterion (BIC). To complement the parametric approach, Kaplan–Meier survival analysis was employed to provide non-parametric insights into storm-free intervals. Additionally, a simulation-based framework estimates storm recurrence probabilities and assesses financial risks through probable maximum loss (PML) calculations. The proposed methodology is applied to the Billion-Dollar Weather and Climate Disasters dataset, compiled by the U.S. National Oceanic and Atmospheric Administration (NOAA). The results demonstrate the model’s effectiveness in predicting severe storm recurrence intervals, offering valuable tools for managing risk in the property and casualty insurance industry.