bluuebunny/crossref_metadata_2025 huggingface dataset:
Correlations between Quality of Life, School Bullying, and Suicide in Adolescents with Attention-Deficit Hyperactivity Disorder
Authors: Chen Yi-Lung , Ho Hsing-Ying , Hsiao Ray C. , Lu Wei-Hsin , Yen Cheng-Fang
Published: May 2020

Although adolescents with attention-deficit hyperactivity disorder (ADHD) have a higher risk of suicidality and more problems related to school bullying, and quality of life (QoL) is reportedly associated with school bullying, suicide, and ADHD, no study has examined their correlation. This study examined the complex relationships between QoL, school bullying, suicide, and ADHD symptoms. A total of 203 adolescents with ADHD aged between 12 and 18 years were recruited. School bullying and QoL were examined using the Chinese version of the School Bullying Experience Questionnaire and the Taiwanese Quality of Life Questionnaire for Adolescents. Network model analysis was conducted to graphically present their relationships. We identified triangular correlations between school bullying, QoL, and suicidality, indicating possible pathways from school bullying to suicidality, and the originating or mediating roles of personal competence and psychological well-being. Furthermore, the ADHD symptoms of inattention and hyperactivity/impulsivity may differentially regulate these pathways. Longitudinal studies are warranted to confirm these findings.
Examining the Effects of Overtime Work on Subjective Social Status and Social Inclusion in the Chinese Context
Authors: Chen Yashuo , Li Pengbo , Yang Chunjiang
Published: May 2020

Although researchers have argued that long work hours have been shown to threaten individual health, lead to work-family conflict, and reduce job performance, the effect of overtime work on social-related outcomes has received little attention. Based on the framework of relative deprivation, we attempt to address this important issue by exploring whether, why, and when individuals’ overtime work influences their social attitudes. By using the data of 400 Chinese employees from the China Labor-Force Dynamics Survey (CLD), we found that overtime work was associated with a low level of subjective social status and social inclusion. In addition, we found that the time type of overtime work (work overtime on weekdays or on weekends and holidays) has a moderating effect on the relationship between overtime work and social inclusion. That is, employees who work overtime on weekdays are unlikely to have a sense of social inclusion. Furthermore, the negative relationship between overtime work and subjective social status was stronger at a low level of fairness rather than a high level of fairness. In contrast, the negative relationship between overtime work and social inclusion was stronger at a high level of fairness rather than a low level of fairness. These findings highlight the critical role of overtime work in social life and also provide novel insights into social intervention aimed at the happiness and harmony of a society.
Green Supplier Selection Based on Green Practices Evaluated Using Fuzzy Approaches of TOPSIS and ELECTRE with a Case Study in a Chinese Internet Company
Authors: Qu Guohua , Zhang Zhijie , Qu Weihua , Xu Zeshui
Published: May 2020

The selection of appropriate green chain suppliers is a very critical decision for effective and efficient green supply chain management in today’s increased awareness and significant environmental pressures from various stakeholders. The aim of this paper is to screen appropriate green chain suppliers based on a framework using fuzzy TOPSIS and ELECTRE for a Chinese internet company. The framework is proposed, grounded on a literature review on green supply chain management practices, after which an empirical analysis is made to be applied an integrated suppliers selection, based on green practices incorporating specifically data collected of the 12 criteria from a set of 12 available suppliers. We use a fuzzy TOPSIS and ELECTRE approach to rank the green chain suppliers, and the results of the proposed framework are compared with the ranks obtained by both the outranking degrees and the incomparability among the actions of fuzzy ELECTRE methodology. Finally, sensitivity analysis was conducted to test the feasibility of the best alternative. The results indicated that the best supplier was alternative 9, and there were four dominant criteria: management support for GSCM, used environmentally friendly materials, followed legal environmental requirements and policies, and reduced the use of harmful substances.
Gardening in Ashes: The Possibilities and Limitations of Gardening to Support Indigenous Health and Well-Being in the Context of Wildfires and Colonialism
Authors: Timler Kelsey , Sandy Dancing Water
Published: May 2020

In this paper, we will discuss gardening as a relationship with nature and an ongoing process to support Indigenous health and well-being in the context of the climate crisis and increasingly widespread forest fires. We will explore the concept of gardening as both a Euro-Western agriculture practice and as a longstanding Indigenous practice—wherein naturally occurring gardens are tended in relationship and related to a wider engagement with the natural world — and the influences of colonialism and climate change on both. Drawing on our experiences as an Indigenous Knowledge Keeper (Dancing Water) and a non-Indigenous community-based researcher (Kelsey), our dialogue will outline ways to support health and well-being through land-based activities that connect with Indigenous traditions in ways that draw on relationships to confront colonialism and the influences of climate change. This dialogue is founded on our experiences in the central interior of British Columbia, Canada, one of the areas hit hardest by the 2017 wildfires. We will explore the possibilities and limitations of gardening and the wider concept of reciprocity and relationship as a means to support food security, food sovereignty, and health for Indigenous Peoples.
Biological Maturation and Hormonal Markers, Relationship to Neuromotor Performance in Female Children
Authors: de Almeida-Neto Paulo Francisco , Silva Dantas Paulo Moreira , Pinto Vanessa Carla Monteiro , Cesário Tatianny de Macêdo , Ribeiro Campos Nathália Monastirski , Santana Eduardo Estevan , de Matos Dihogo Gama , Aidar Felipe J , Tinoco Cabral Breno Guilherme de Araújo
Published: May 2020

Background: Mechanisms that influence muscle strength can interfere with neuromotor performance and overall health, thus hormone markers and maturation can interact in this process. Objective: The present study aimed to verify the relationship of hormonal markers and biological maturation on neuromotor abilities in young people. Methods: This is a cross-sectional study with 44 female participants (11.5 ± 1.5 years). Hormones were analyzed biochemically. Skeletal and somatic maturation were analyzed using anthropometry. The muscular power of the upper and lower limbs, body speed with change of direction, and speed of the upper limbs were verified. Results: Bone age was correlated with hormonal markers (estradiol: r = 0.58; p = 0.0007), (testosterone: r = 0.51; p = 0.005). Peak growth velocity correlated with estradiol (r = 0.51; p = 0.004). The power of the lower limbs (estradiol: r = 0.52; p = 0.006; testosterone: r = 0.42; p = 0.03) and of the upper limbs (estradiol: r = 0.51; p = 0.007; testosterone: r = 0.42; p = 0.02) had a positive correlation with hormone levels and had similar results with maturation. The analysis by artificial neural networks indicated that the maturation can predict the neuromotor performance between 57.4% and 76%, while the hormonal markers showed a potential of more than 95% for the foreshadowing of the neuromotor performance of the upper limbs. Conclusion: It was possible to conclude that the hormones had a relationship with maturational development and bone age in female subjects.
Physical Activity in 15–17-Year-Old Adolescents as Compensation for Sedentary Behavior in School
Authors: Jakubec Lukáš , Frömel Karel , Chmelík František , Groffik Dorota
Published: May 2020

The traditional concept of education and school settings significantly contribute to the sedentary behavior of adolescents at secondary schools. The aim of this study is to identify the volume and intensity of physical activity (PA) that adolescent boys and girls engage in during recesses, after school, and during the day to compensate for sedentary behavior in lessons. The study was conducted at 29 Czech and 9 Polish schools. The study involved 868 girls and 409 boys aged 15–17 years. An ActiTrainerTM accelerometer was used to monitor PA and heart rate. Participants were divided into four quartile groups. Most sedentary boys and girls had less PA and showed a worse ratio of physical inactivity (PI)/PA than non-sedentary participants during recesses. In the after-school period, there were no significant differences. On school days, most sedentary boys and girls showed lower PA, a worse ratio of PI/PA, fewer steps·hour−1, and lower energy expenditure than their non-sedentary counterparts. Vigorous PA of ≥8 METs was reached by 48% of most sedentary boys (75% non-sedentary) and 47% of most sedentary girls (54% non-sedentary). Most sedentary adolescents do not compensate for their sedentary behavior in lessons with higher PA intensity or volume during recesses, after-school, or in overall daily PA.
Surface-Modified Sewage Sludge-Derived Carbonaceous Catalyst as a Persulfate Activator for Phenol Degradation
Authors: Han Meiling , Zhang Jin , Chu Wen , Zhou Gongfu , Chen Jiahao
Published: May 2020

In this study, a catalytic persulfate oxidation process comprising sodium persulfate (PS) and modified sewage sludge-derived carbonaceous catalysts was tested for the degradation of phenol. Sludge-based biochar was modified by high-temperature treatment combined with hydrochloric acid oxidation. The surface properties of carbonaceous catalysts before and after modification were characterized by elemental analysis, N2 isothermal adsorption-desorption, scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy. The effects of reaction parameters including catalyst dosage, PS/phenol molar ratio, initial pH and reaction temperature on the degradation rate of phenol were investigated. The kinetics of phenol transformation was explored and the reaction rate appeared pseudo first-order kinetics. In SS-600-HCl/PS system, 91% phenol could be efficiently degraded under certain reaction conditions ([phenol]0 = 100 mg/L, catalyst dosage = 0.8 g/L, PS/phenol molar ratio = 3/1, pH = 7, 25 °C) in 180 min. Thus, the results showed that the modified sewage sludge-derived carbonaceous catalyst had a better ability to activate PS for phenol degradation.
Nasopharyngeal and Oropharyngeal Swabs, And/Or Serology for SARS COVID-19: What Are We Looking For?
Authors: Sanduzzi Alessandro , Sanduzzi Zamparelli Stefano
Published: May 2020

Governments and clinicians that were fully involved in the dramatic SARS-CoV-2 outbreak during the last few weeks in Italy (and more or less all over the world) are fiercely debating the use of methods for screening this viral infection. Thus, all countries are employing a lot of resources in order to test more and more subjects. For this purpose, there are different strategies, based on either direct or indirect tests. Among the first category, the main assays used for SARS-CoV-2 are based on a real-time reverse transcriptase polymerase chain reaction (RT-PCR). Such tests can be performed on nasopharyngeal and oropharyngeal swabs for the categories of those with symptoms and those potentially exposed. In order to integrate the molecular assays in the diagnosis of SARS-CoV-2, a wide range of serology immunoassays (IAs) have also been developed. If we want to identify “immune” people in order to let them to come back to work, serology is the best (and probably the only) approach.
Coordination of a Dual-Channel Pharmaceutical Supply Chain Based on the Susceptible-Infected-Susceptible Epidemic Model
Authors: Hou Yanhong , Wang Fan , Chen Zhitong , Shi Victor
Published: May 2020

With the continuous development of Internet, online pharmaceutical channels in many countries have seen rapid expansion. As a result, pharmaceutical supply chain participants can adopt dual channels, namely, both online channels and offline channels. As online channels compete with traditional offline channels, it is of great relevance to study the potential conflicts and coordination between them, which is the focus of this paper. Specifically, this article develops a susceptible-infected-susceptible epidemic model of the dual channels for a pharmaceutical supply chain. Our main findings are that in a competitive situation, there is a positive stable equilibrium. Furthermore, increasing the rate of influence of offline transmission, online transmission, and cross transmission will improve sales. Moreover, improving the transmission influence rate will turn more potential customers into purchasers, increase channel sales, and achieve dual channel coordination. We then conduct numerical analysis to illustrate and complement the findings from the model. Finally, we provide managerial insights for implementing successful dual-channel pharmaceutical supply chains.
Targeting RGS4 Ablates Glioblastoma Proliferation
Authors: Guda Maheedhara R. , Velpula Kiran K. , Asuthkar Swapna , Cain Charlie P. , Tsung Andrew J.
Published: May 2020

Glioblastoma (GBM) is the most common type of adult primary brain tumor with a median survival rate of less than 15 months, regardless of the current standard of care. Cellular heterogeneity, self-renewal ability and tumorigenic glioma cancer stem cell (GSC) populations contribute to the difficulty in treating GBM. G-protein-coupled receptors (GPCRs) are the largest group of membrane proteins and mediate many cellular responses. Regulators of G-protein signaling 4 (RGS4) are negative regulators of G-protein signaling, and elevated levels of RGS4 are reportedly linked with several human diseases, including cancer. This study investigates the effect of silencing RGS4, resulting in inhibition of GSC growth, invasion and migration. Data obtained from The Cancer Genome Atlas (TCGA) demonstrated poor patient survival with high expression of RGS4. Immunohistochemistry and immunoblot analysis conducted on GBM patient biopsy specimens demonstrated increased RGS4 expression correlative with the TCGA data. RNA sequencing confirmed a significant decrease in the expression of markers involved in GSC invasion and migration, particularly matrix metalloproteinase-2 (MMP2) in knockout of RGS4 using CRISPR plasmid (ko-RGS4)-treated samples compared to parental controls. Gelatin zymography confirmed the reduced activity of MMP2 in ko-RGS4-treated samples. Silencing RGS4 further reduced the invasive and migratory abilities and induction of apoptosis of GSCs as evidenced by Matrigel plug assay, wound healing assay and human apoptosis array. Collectively, our results showed that the silencing of RGS4 plays an important role in regulating multiple cellular functions, and is an important therapeutic target in GBM.
Construction of an Electrochemical Receptor Sensor Based on Graphene/Thionine for the Sensitive Determination of β-Lactam Antibiotics Content in Milk
Authors: Wang Lei , Zhang Liyun , Wang Yuke , Ou Yahong , Wang Xu , Pan Yuanhu , Wang Yulian , Huang Lingli , Cheng Guyue , Xie Shuyu , Chen Dongmei , Tao Yanfei
Published: May 2020

In antibiotics, β-lactam is one kind of major concern acknowledged as an unavoidable contaminant in milk. Thus, a facile and sensitive method is essential for rapid β-lactam antibiotics detection. In our work, a specific electrochemical receptor sensor based on the graphene/thionine (GO/TH) composite was established. The mechanism of the electrochemical receptor sensor was a direct competitive inhibition of the binding of horseradish peroxidase-labeled ampicillin (HRP-AMP) to the mutant BlaR-CTD protein by free β-lactam antibiotics. Then, horseradish peroxidase (HRP) catalyzed the hydrolysis of the substrate hydrogen peroxide (H2O2), which produced an electrochemical signal. Under optimal experimental conditions, this method could quantitatively detect cefquinome from 0.1 to 8 μg L−1 and with the limit of detection (LOD) of 0.16 μg L−1, much lower than the maximum residue limit (MRL) of 5 μg L−1 set by the European Union. In addition, the LOD of spiked milk samples with cefalexin, cefquinoxime, cefotafur, penicillin G and ampicillin were 14.88 μg L−1, 2.46 μg L−1, 17.16 μg L−1, 0.06 μg L−1, 0.21 μg L−1 and the limits of quantitation (LOQ) were 36.09 μg L−1, 5.40 μg L−1, 41.45 μg L−1, 0.13 μg L−1, 0.42 μg L−1, respectively. The sensor showed a favorable recovery of 84.89–102.44%. Moreover, the electrochemical receptor sensor was successfully applied to assay β-lactam antibiotics in milk, which showed good correlation with the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS).
Hypermethylation of the TSPOAP1-AS1 Promoter May Be Associated with Obesity in Overweight/Obese Korean Subjects
Authors: Yim Nam-Hui , Cha Min Ho , Kim Myung Sunny
Published: May 2020

Obesity is a major chronic disease associated with the risk of serious cardiovascular or endocrinal diseases, such as hypertension, diabetes, atherosclerosis and stroke. Considerable interest has been directed towards the potential effects of epigenetic variations in obesity. In this study, we evaluated DNA methylation level at the promoter region of the gene encoding TSPO-associated protein 1 antisense RNA 1 (TSPOAP1-AS1) in 80 overweight/obese subjects (body mass index (BMI) > 25) and 104 non-obese subjects who participated in the SOPI-Stroke study in Korea. DNA methylation was measured using bisulfite amplicon sequencing (BSAS). A general linear model or relative correlation was used to determine the effects of DNA methylation on obesity and obese phenotypes. Notably, the mean level of DNA methylation was significantly higher in the overweight/obese group than in the non-obese group (18.62% vs. 17.18%). Further analyses revealed significant positive correlations of the BMI, the serum total cholesterol and low-density lipoprotein cholesterol levels with the DNA methylation level (p = 0.0493, p = 0.003, and p = 0.0094, respectively). The study findings suggest an association between DNA methylation at the TSPOAP1-AS1 promoter and overweight/obesity. Accordingly, methylation in this promoter region might be a potential predictor of obesity.
TRPV1 Responses in the Cerebellum Lobules V, VIa and VII Using Electroacupuncture Treatment for Inflammatory Hyperalgesia in Murine Model
Authors: Inprasit Chanya , Lin Yi-Wen
Published: May 2020

Inflammatory pain sensation is an important symptom which protects the body against additional tissue damage and promotes healing. Discovering long-term and effective treatments for pain remains crucial in providing efficient healthcare. Electroacupuncture (EA) is a successful therapy used for pain relief. We aimed to investigate effects and mechanisms of Complete Freund’s Adjuvant (CFA)-inducing inflammatory pain in the cerebellum, and the inhibition of this inflammatory hyperalgesia using EA at Zusanli acupoint (ST36). The results display a significant increase in mechanical and thermal sensitivities in the CFA and CFA + SHAM groups, which was significantly reduced in the CFA+EA and CFA + KO groups. This evidence was substantiated in the protein levels observed using immunoblotting, and presented with significant escalations after CFA inducing inflammatory hyperalgesia in CFA and CFA + SHAM groups. Then, they were significantly attenuated by EA in the CFA + EA group. Furthermore, the CFA + transient receptor vanilloid member 1 (TRPV1)−/− group indicated similar significant decreases of protein expression. Additionally, a concomitant overexpression in lobule VIa was also observed in immunofluorescence. These consequences suggest that CFA-induced inflammatory pain provokes modifications in cerebellum lobules V, VIa and VII, which can subsequently be regulated by EA treatment at the ST36 through its action on TRPV1 and related molecular pathways.
Arachidonic Acid Attenuates Cell Proliferation, Migration and Viability by a Mechanism Independent on Calcium Entry
Authors: Cantonero Carlos , Sánchez-Collado Jose , Lopez Jose J. , Salido Ginés M. , Rosado Juan A. , Redondo Pedro C.
Published: May 2020

Arachidonic acid (AA) is a phospholipase A2 metabolite that has been reported to mediate a plethora of cellular mechanisms involved in healthy and pathological states such as platelet aggregation, lymphocyte activation, and tissue inflammation. AA has been described to activate Ca2+ entry through the arachidonate-regulated Ca2+-selective channels (ARC channels). Here, the analysis of the changes in the intracellular Ca2+ homeostasis revealed that, despite MDA-MB-231 cells expressing the ARC channel components Orai1, Orai3, and STIM1, AA does not evoke Ca2+ entry in these cells. We observed that AA evokes Ca2+ entry in MDA-MB-231 cells transiently expressing ARC channels. Nevertheless, MDA-MB-231 cell treatment with AA reduces cell proliferation and migration while inducing cell death through apoptosis. The latter mostly likely occurs via mitochondria membrane depolarization and the activation of caspases-3, -8, and -9. Altogether, our results indicate that AA exerts anti-tumoral effects on MDA-MB-231 cells, without having any effect on non-tumoral breast epithelial cells, by a mechanism that is independent on the activation of Ca2+ influx via ARC channels.
Gold Nanocomplex Strongly Modulates the PI3K/Akt Pathway and Other Pathways in MCF-7 Breast Cancer Cell Line
Authors: Mahmoud Nouf N. , Abuarqoub Duaa , Zaza Rand , Sabbah Dima A. , Khalil Enam A. , Abu-Dahab Rana
Published: May 2020

Conjugating drugs with gold nanoparticles (GNP) is a key strategy in cancer therapy. Herein, the potential inhibition of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway, and other pathways of the MCF-7 cell-line, was investigated upon treatment with gold nanorods (GNR) conjugated with a PI3K inhibitor drug. The results revealed that the coupling of GNR with the drug drastically modulated the expression of PI3Kα at the gene and protein levels compared to the drug or GNR alone. The PI3Kα pathway is involved in tumor progression and development through the mediation of different mechanisms such as apoptosis, proliferation, and DNA damage. Treatment with the nanocomplex significantly affected the gene expression of several transcription factors responsible for cell growth and proliferation, apoptotic pathways, and cell cycle arrest. Furthermore, the gene expression of different regulatory proteins involved in cancer progression and immune responses were significantly modified upon treatment with the nanocomplex compared to the free drug or GNR alone.
Epigallocatechin Gallate Enhances MAL-PDT Cytotoxic Effect on PDT-Resistant Skin Cancer Squamous Cells
Authors: León Daniela , Buchegger Kurt , Silva Ramón , Riquelme Ismael , Viscarra Tamara , Mora-Lagos Bárbara , Zanella Louise , Schafer Fabiola , Kurachi Cristina , Roa Juan Carlos , Ili Carmen , Brebi Priscilla
Published: May 2020

Photodynamic therapy (PDT) has been used to treat certain types of non-melanoma skin cancer with promising results. However, some skin lesions have not fully responded to this treatment, suggesting a potential PDT-resistant phenotype. Therefore, novel therapeutic alternatives must be identified that improve PDT in resistant skin cancer. In this study, we analyzed the cell viability, intracellular protoporphyrin IX (PpIX) content and subcellular localization, proliferation profile, cell death, reactive oxygen species (ROS) detection and relative gene expression in PDT-resistant HSC-1 cells. PDT-resistant HSC-1 cells show a low quantity of protoporphyrin IX and low levels of ROS, and thus a low rate of death cell. Furthermore, the resistant phenotype showed a downregulation of HSPB1, SLC15A2, FECH, SOD2 and an upregulation of HMBS and BIRC5 genes. On the other hand, epigallocatechin gallate catechin enhanced the MAL-PDT effect, increasing levels of protoporphyrin IX and ROS, and killing 100% of resistant cells. The resistant MAL-PDT model of skin cancer squamous cells (HSC-1) is a reliable and useful tool to understand PDT cytotoxicity and cellular response. These resistant cells were successfully sensitized with epigallocatechin gallate catechin. The in vitro epigallocatechin gallate catechin effect as an enhancer of MAL-PDT in resistant cells is promising in the treatment of difficult skin cancer lesions.
Immunomodulation in Cystic Fibrosis: Why and How?
Authors: Giacalone Vincent D. , Dobosh Brian S. , Gaggar Amit , Tirouvanziam Rabindra , Margaroli Camilla
Published: May 2020

Cystic fibrosis (CF) lung disease is characterized by unconventional mechanisms of inflammation, implicating a chronic immune response dominated by innate immune cells. Historically, therapeutic development has focused on the mutated cystic fibrosis transmembrane conductance regulator (CFTR), leading to the discovery of small molecules aiming at modulating and potentiating the presence and activity of CFTR at the plasma membrane. However, treatment burden sustained by CF patients, side effects of current medications, and recent advances in other therapeutic areas have highlighted the need to develop novel disease targeting of the inflammatory component driving CF lung damage. Furthermore, current issues with standard treatment emphasize the need for directed lung therapies that could minimize systemic side effects. Here, we summarize current treatment used to target immune cells in the lungs, and highlight potential benefits and caveats of novel therapeutic strategies.
Polysaccharide-Based Injection Matrix for Serial Crystallography
Authors: Nam Ki Hyun
Published: May 2020

Serial crystallography (SX) provides an opportunity to observe the molecular dynamics of macromolecular structures at room temperature via pump-probe studies. The delivery of crystals embedded in a viscous medium via an injector or syringe is widely performed in synchrotrons or X-ray free-electron laser facilities with low repetition rates. Various viscous media have been developed; however, there are cases in which the delivery material undesirably interacts chemically or biologically with specific protein samples, or changes the stability of the injection stream, depending on the crystallization solution. Therefore, continued discovery and characterization of new delivery media is necessary for expanding future SX applications. Here, the preparation and characterization of new polysaccharide (wheat starch (WS) and alginate)-based sample delivery media are introduced for SX. Crystals embedded in a WS or alginate injection medium showed a stable injection stream at a flow rate of < 200 nL/min and low-level X-ray background scattering similar to other hydrogels. Using these media, serial millisecond crystallography (SMX) was performed, and the room temperature crystal structures of glucose isomerase and lysozyme were determined at 1.9–2.0 Å resolutions. WS and alginate will allow an expanded application of sample delivery media in SX experiments.
Legume Genetics and Biology: From Mendel’s Pea to Legume Genomics
Authors: Smýkal Petr , von Wettberg Eric J.B. , McPhee Kevin
Published: May 2020

Legumes have played an important part in cropping systems since the dawn of agriculture, both as human food and as animal feed. The legume family is arguably one of the most abundantly domesticated crop plant families. Their ability to symbiotically fix nitrogen and improve soil fertility has been rewarded since antiquity and makes them a key protein source. The pea was the original model organism used in Mendel’s discovery of the laws of inheritance, making it the foundation of modern plant genetics. This Special Issue provides up-to-date information on legume biology, genetic advances, and the legacy of Mendel.
Myeloproliferative Diseases as Possible Risk Factor for Development of Chronic Thromboembolic Pulmonary Hypertension—A Genetic Study
Authors: Eichstaedt Christina A. , Verweyen Jeremias , Halank Michael , Benjamin Nicola , Fischer Christine , Mayer Eckhard , Guth Stefan , Wiedenroth Christoph B. , Egenlauf Benjamin , Harutyunova Satenik , Xanthouli Panagiota , Marra Alberto M. , Wilkens Heinrike , Ewert Ralf , Hinderhofer Katrin , Grünig Ekkehard
Published: May 2020

Chronic thromboembolic pulmonary hypertension (CTEPH) is a rare disease which is often caused by recurrent emboli. These are also frequently found in patients with myeloproliferative diseases. While myeloproliferative diseases can be caused by gene defects, the genetic predisposition to CTEPH is largely unexplored. Therefore, the objective of this study was to analyse these genes and further genes involved in pulmonary hypertension in CTEPH patients. A systematic screening was conducted for pathogenic variants using a gene panel based on next generation sequencing. CTEPH was diagnosed according to current guidelines. In this study, out of 40 CTEPH patients 4 (10%) carried pathogenic variants. One patient had a nonsense variant (c.2071A>T p.Lys691*) in the BMPR2 gene and three further patients carried the same pathogenic variant (missense variant, c.1849G>T p.Val617Phe) in the Janus kinase 2 (JAK2) gene. The latter led to a myeloproliferative disease in each patient. The prevalence of this JAK2 variant was significantly higher than expected (p < 0.0001). CTEPH patients may have a genetic predisposition more often than previously thought. The predisposition for myeloproliferative diseases could be an additional risk factor for CTEPH development. Thus, clinical screening for myeloproliferative diseases and genetic testing may be considered also for CTEPH patients.
18F-FDG PET-Based Imaging of Myocardial Inflammation Following Acute Myocardial Infarction in a Mouse Model
Authors: Vasudevan Praveen , Gäbel Ralf , Stenzel Jan , Förster Joanna , Kurth Jens , Vollmar Brigitte , Krause Bernd Joachim , Ince Hüseyin , David Robert , Lang Cajetan Immanuel
Published: May 2020

Cellular inflammation is an integral part of the healing process following acute myocardial infarction and has been under intense investigation for both therapeutic and prognostic approaches. Monocytes and macrophages are metabolically highly active and show increased uptake rates of glucose and its analog, 18F-FDG. Yet, the specific allocation of the radioactivity to the inflammatory cells via positron emission tomography (PET) imaging requires the suppression of glucose metabolism in viable myocardium. In mice, the most important model organism in basic research, this can be achieved by the application of ketamine/xylazine (KX) for anesthesia instead of isoflurane. Yet, while the consensus exists that glucose metabolism is effectively suppressed, a strategy for reproducible image analysis is grossly lacking and causes uncertainty concerning data interpretation. We introduce a simple strategy for systematic image analysis, which is a prerequisite to evaluate therapies targeting myocardial inflammation. Mice underwent permanent occlusion of the left anterior descending artery (LAD), inducing an acute myocardial infarction (MI). Five days after MI induction, 10MBq 18F-FDG was injected intravenously and a static PET/CT scan under ketamine/xylazine anesthesia was performed. For image reconstruction, we used an algorithm based on three-dimensional ordered subsets expectation maximization (3D-OSEM) followed by three-dimensional ordinary Poisson maximum a priori (MAP) reconstruction. Using this approach, high focal tracer uptake was typically located in the border zone of the infarct by visual inspection. To precisely demarcate the border zone for reproducible volume of interest (VOI) positioning, our protocol relies on positioning VOIs around the whole left ventricle, the inferobasal wall and the anterolateral wall guided by anatomical landmarks. This strategy enables comparable data in mouse studies, which is an important prerequisite for using a PET-based assessment of myocardial inflammation as a prognostic tool in therapeutic applications.
A Synergistic Effect of Reactive Oxygen and Reactive Nitrogen Species in Plasma Activated Liquid Media Triggers Astrocyte Wound Healing
Authors: Sardella Eloisa , Mola Maria Grazia , Gristina Roberto , Piccione Monica , Veronico Valeria , De Bellis Manuela , Cibelli Antonio , Buttiglione Maura , Armenise Vincenza , Favia Pietro , Nicchia Grazia Paola
Published: May 2020

Astrocyte proliferation and migration toward injured Central Nervous System (CNS) areas are key features of astrogliosis and glial scar formation. Even though it is known that intracellular and environmental Reactive Oxygen and Nitrogen Species (RONS) affect astrocyte behaviour in physiological and pathophysiological conditions, their effects on the migration and growth of astrocytes are still unclear. Plasma-technologies are emerging in medicine as a tool to generate RONS for treating cells directly or through Plasma Activated Liquid Media (PALM). In this paper, we show for the first time how the use of PALM can modulate both astrocyte growth and migration as a function of active species produced by plasma in liquids. Our results show that PALM, generated by means of cold atmospheric pressure plasmas fed with N2, air or O2, can modulate astrocyte behaviour depending on the content of hydrogen peroxide and nitrite in the liquid. In particular, H2O2 enriched PALM induced a negative effect on cell growth associated with the mild wound healing improvement of primary astrocytes, in a scratch assay. Nitrite enriched PALM induced a selective effect on the wound healing without affecting cell growth. PALM containing a more balanced level of H2O2 and NO2− were able to affect cell growth, as well as significantly ameliorate wound healing. None of the PALM investigated induced upregulation of the gliotic inflammatory marker glial fibrillary acidic protein (GFAP), or of the astrocyte markers Aquaporin-4 (AQP4) and Connexin-43 (Cx-43) analysed by Western blot. Finally, immunofluorescence analysis revealed the presence of NO2- able to induce elongated protrusions at the front end of wounded astrocytes in the direction of cell migration. With our study we believe to have shown that PALM offer a novel tool to modulate astrocyte behaviour and that they are promising candidates for controlling astrogliosis in the case of CNS injuries.
Mechanisms of Blood–Brain Barrier Dysfunction in Traumatic Brain Injury
Authors: Cash Alison , Theus Michelle H.
Published: May 2020

Traumatic brain injuries (TBIs) account for the majority of injury-related deaths in the United States with roughly two million TBIs occurring annually. Due to the spectrum of severity and heterogeneity in TBIs, investigation into the secondary injury is necessary in order to formulate an effective treatment. A mechanical consequence of trauma involves dysregulation of the blood–brain barrier (BBB) which contributes to secondary injury and exposure of peripheral components to the brain parenchyma. Recent studies have shed light on the mechanisms of BBB breakdown in TBI including novel intracellular signaling and cell–cell interactions within the BBB niche. The current review provides an overview of the BBB, novel detection methods for disruption, and the cellular and molecular mechanisms implicated in regulating its stability following TBI.
Comparative Analysis among Discrete Fourier Transform, K-Means and Artificial Neural Networks Image Processing Techniques Oriented on Quality Control of Assembled Tires
Authors: Massaro Alessandro , Dipierro Giovanni , Cannella Emanuele , Galiano Angelo Maurizio
Published: May 2020

The present paper discusses a comparative application of image processing techniques, i.e., Discrete Fourier Transform, K-Means clustering and Artificial Neural Network, for the detection of defects in the industrial context of assembled tires. The used Artificial Neural Network technique is based on Long Short-Term Memory and Fully Connected neural networks. The investigations focus on the monitoring and quality control of defects, which may appear on the external surface of tires after being assembled. Those defects are caused from tires which are not properly assembled to their respective metallic wheel rim, generating deformations and scrapes which are not desired. The proposed image processing techniques are applied on raw high-resolution images, which are acquired by in-line imaging and optical instruments. All the described techniques, i.e., Discrete Fourier Transform, K-Means clustering and Long Short-Term Memory, were able to determine defected and acceptable external tire surfaces. The proposed research is taken in the context of an industrial project which focuses on the development of automated quality control and monitoring methodologies, within the field of Industry 4.0 facilities. The image processing techniques are thus meant to be adopted into production processes, giving a strong support to the in-line quality control phase.
Telecommunication Systems for Small Satellites Operating at High Frequencies: A Review
Authors: Babuscia Alessandra
Published: May 2020

Small Satellites and in particular CubeSats are becoming extremely popular platforms with which to perform space research. They allow for rapid prototyping with considerable cost savings with respect to traditional missions. However, as small satellite missions become more ambitious in terms of destinations to reach (from Low Earth Orbit to interplanetary) and in terms of the amount of data to transmit, new technologies need to be developed to provide adequate telecommunication support. This paper aims to review the telecommunication systems that have been developed at the Jet Propulsion Laboratory for some of the most recent CubeSat missions operating at different frequency bands: ASTERIA (S-Band), MarCO (X-Band and UHF) and ISARA (Ka-Band and UHF). For each of these missions: the telecommunication challenges and requirements are listed; the final system design is presented; the characteristics of the different hardware components are shown; and the lessons learned through operations are discussed.
Detection of Circulating Tumor Cells in Renal Cell Carcinoma: Disease Stage Correlation and Molecular Characterization
Authors: Klezl Petr , Pospisilova Eliska , Kolostova Katarina , Sonsky Jindrich , Maly Ondrej , Grill Robert , Pawlak Ireneusz , Bobek Vladimir
Published: May 2020

The presence of circulating tumor cells (CTCs) in patients with solid tumors is associated with poor prognosis. However, there are limited data concerning the detection of CTCs in renal cell cancer (RCC). The aim of this study is to evaluate the presence of CTCs in peripheral blood of patients with RCC undergoing surgery (n = 186). CTCs were tested before and after surgery as well as during the follow-up period afterwards. In total 495 CTC testing in duplicates were provided. To enrich CTCs, a size-based separation protocol and tube MetaCell® was used. CTCs presence was evaluated by single cell cytomorphology based on vital fluorescence microscopy. Additionally, to standardly applied fluorescence stains, CTCs viability was controlled by mitochondrial activity. CTCs were detected independently on the sampling order in up to 86.7% of the tested blood samples in patients undergoing RCC surgery. There is higher probability of CTC detection with growing tumor size, especially in clear cell renal cell cancer (ccRCC) cases. Similarly, the tumor size corresponds with metastasis presence and lymph node positivity and CTC detection. This paper describes for the first-time successful analysis of viable CTCs and their mitochondria as a part of the functional characterization of CTCs in RCC.
Volumetric Carotid Flow Characteristics in Doppler Ultrasonography in Healthy Population Over 65 Years Old
Authors: Kaszczewski Piotr , Elwertowski Michal , Leszczynski Jerzy , Ostrowski Tomasz , Galazka Zbigniew
Published: May 2020

Background: Carotid flow velocity criteria are well established, with age being a factor influencing measurements. However, there are no volumetric standards for the flow in extracranial arteries. The aim of the study was related to volumetric flow assessment of extracranial arteries in a healthy population >65 years old. Methods: Doppler volumetric measurements of internal carotid (ICA), external carotid (ECA) and vertebral arteries (VA) were performed in 123 healthy volunteers >65 years old and compared with 56 healthy volunteers <65 years old. Results: The continuous decline in cerebral blood flow (CBF) volume was observed (p < 0.00001). Volumetric reference values were established in study groups: 1., 65–69 years: 898.5 ± 119.1; 2., 70–74 years: 838.5 ± 148.9; 3., 75–79 years: 805.1 ± 99.3; 4., >80 years: 685.7 ± 112.3 (mL/min). Significant differences were observed between groups: 1 and 3.4, as well as 3 and 4 (p = 0.0295, < 0.000001, 0.00446 respectively). CBF volume decreases gradually with age: 28–64 years—6.2 mL/year (p = 0.0019), 65–75 years—11.4 mL/year (p = 0.0121) and >75 years—14.3 mL/year (p = 0.0074). This is a consequence of flow volume decline in ICA (p = 0.00001) and to lesser extent ECA (p = 0.0011). The decrease of peak systolic (p = 0.002) and end diastolic (p = < 0.00001) velocities in ICA and peak systolic velocity in ECA (p = 0.0017) were observed. Conclusions: CBF decreases with ageing. Volumetric assessment of CBF may play an important additional role in diagnostics of patients with carotid stenosis. Doppler assessment of cerebral flow volume may create an interesting tool for identifying patients with diminished cerebrovascular reserve and higher risk of ischemic symptoms occurrence.
Modification of PLA-Based Films by Grafting or Coating
Authors: Miletić Aleksandra , Ristić Ivan , Coltelli Maria-Beatrice , Pilić Branka
Published: May 2020

Recently, the demand for the use of natural polymers in the cosmetic, biomedical, and sanitary sectors has been increasing. In order to meet specific functional properties of the products, usually, the incorporation of the active component is required. One of the main problems is enabling compatibility between hydrophobic and hydrophilic surfaces. Therefore, surface modification is necessary. Poly(lactide) (PLA) is a natural polymer that has attracted a lot ofattention in recent years. It is bio-based, can be produced from carbohydrate sources like corn, and it is biodegradable. The main goal of this work was the functionalization of PLA, inserting antiseptic and anti-inflammatory nanostructured systems based on chitin nanofibrils–nanolignin complexes ready to be used in the biomedical, cosmetics, and sanitary sectors. The specific challenge of this investigation was to increase the interaction between the hydrophobic PLA matrix with hydrophilic chitin–lignin nanoparticle complexes. First, chemical modification via the “grafting from” method using lactide oligomers was performed. Then, active coatings with modified and unmodified chitin–lignin nanoparticle complexes were prepared and applied on extruded PLA-based sheets. The chemical, thermal, and mechanical characterization of prepared samples was carried out and the obtained results were discussed.
Reduction of the Gaseous Emissions in the Marine Diesel Engine Using Biodiesel Mixtures
Authors: Puškár Michal , Kopas Melichar , Sabadka Dušan , Kliment Marek , Šoltésová Marieta
Published: May 2020

Taking into consideration the quality of air, it is necessary to ensure a continued reduction of the gaseous emissions that are produced by the maritime transport. The most effective solution of this serious worldwide problem is application of a suitable fuel mixture, which contains a bio-component, i.e. the biofuel. The presented scientific study is focused on influence of the biofuels on production of the gaseous emissions in the case of a diesel auxiliary engine, which is used in the ship transport. There were created various fuel mixtures with different content of the bio-component in order to investigate their emission characteristics. The individual experimental measurements were performed at the different engine loading levels and using a variable engine speed spectrum. The obtained results demonstrated a significant influence of the fuel mixtures on the whole combustion process, on the heat release process, on the pressure time behaviour as well as on the engine emission characteristics.
Hybrid Pectin-Based Sorbents for Cesium Ion Removal
Authors: Bok-Badura Joanna , Jakóbik-Kolon Agata , Kazek-Kęsik Alicja , Karoń Krzysztof
Published: May 2020

In this paper, beads-shaped hybrid sorbents composed of pectin and Prussian blue were prepared. Various ratios of pectin and Prussian blue in hybrid sorbents were tested. Obtained sorbents had high and roughly constant sorption capacity in a broad pH range (4–10), in which also the swelling index and stability of sorbents were satisfactory. The preliminary sorption studies proved that almost 100% of cesium removal efficiency may be achieved by using the proper sorbent dose. The sorption capacity of the hybrid sorbent with a 1:1 ratio of pectin to Prussian blue equaled q = 36.5 ± 0.8 mg/g (dose 3 g/L, pH = 6, temp. = 22 ± 1 °C, t = 24 h). The obtained results showed that the prepared hybrid pectin-based sorbents are promising for cesium ions removal.
Lanchester Models for Irregular Warfare
Authors: Kress Moshe
Published: May 2020

Military operations research and combat modeling apply mathematical models to analyze a variety of military conflicts and obtain insights about these phenomena. One of the earliest and most important set of models used for combat modeling is the Lanchester equations. Legacy Lanchester equations model the mutual attritional dynamics of two opposing military forces and provide some insights regarding the fate of such engagements. In this paper, we review recent developments in Lanchester modeling, focusing on contemporary conflicts in the world. Specifically, we present models that capture irregular warfare, such as insurgencies, highlight the role of target information in such conflicts, and capture multilateral situations where several players are involved in the conflict (such as the current war in Syria).
Diagnosis Method for the Heat Balance State of an Aluminum Reduction Cell Based on Bayesian Network
Authors: Zhu Jiaming , Li Jie
Published: May 2020

The superheat of an electrolyte is an important indicator of the heat balance state of aluminum reduction cells. In industrial practice, it costs too much to accurately measure the superheat in every cell every day. A common alternative is to calculate the superheat based on additive concentrations in the electrolyte, which has problems of high error and long delay. In this paper, a method to diagnose the heat balance state of an aluminum reduction cell based on Bayesian network is presented, a Bayesian network structure and CPT (conditional probability distribution) were built, and the continuous diagnosis process is presented. This diagnosis method takes important symptoms and factors into account, taking advantage of more useful information instead of only calculated superheat. The application examples show that this method is effective in diagnosing the heat balance state for uncertain and incomplete superheat information.