bluuebunny/crossref_metadata_2025 huggingface dataset:
Comitatus 30 év/könyv
Authors: Zsuzsa Darida
Published: February 2022

A Comitatus folyóirat az egyetlen közhatalmi szervektől független vidéken szerkesztett országos önkormányzati szaklap, melynek 30 éves jubileuma alkalmából egy tanulmánykötet szerkesztésére szánták el magukat a szerzők. E tanulmánykötetet mutatom be recenziómban. A kötet meglehetősen színes, számos különböző témát érint. E témák közül példálózó jelleggel kiemelném a Pólusok folyóirat tematikájához kapcsolódó területpolitikát, a megyei és régiós szintű politikákat, a közigazgatás-irányítás és a kormányzás kérdéskörét.
Advances in therapeutic agents targeting quiescent cancer cells
Authors: Nik Nabil Wan Najbah , Xi Zhichao , Liu Mengfan , Li Yang , Yao Mu , Liu Tao , Dong Qihan , Xu Hongxi
Published: February 2022

Quiescent cancer cells (QCCs) reversibly reside in G0 phase, thus allowing them to survive chemotherapy and radiotherapy, which generally target proliferating cells. Surviving QCCs may re-proliferate, and consequently result in cancer progression, recurrence, and metastasis. Therefore, understanding the key players governing QCC survival and activation is crucial for developing QCC-targeting agents. This review presents an overview of (1) the mechanisms underlying the regulation of QCC status and (2) recent advances in the development of QCC-targeting therapeutic agents and their underlying mechanisms. The development of effective therapeutic modalities that target QCCs may enable new cancer treatments to prevent cancer progression and recurrence.
Encounters with “Latin American Art” in Canada
Authors: Sepúlveda Gabriela Aceves
Published: February 2022

This Dialogues section seeks to contribute to the scholarship on Latin American art in Canada and “Latinx Canadian art.” We aim to broaden the historical and current narratives of art and artists from Latin America north of the United States, taking into account Canada’s history of migration and its official bilingual status (French-English), multilingual and multicultural reality, and relationship with Indigenous peoples. Adding to the urgency of studying the presence of Latin American art in Canada, there is also a need to focus on the work of artists and curators with a Latin American background. They are developing languages of expression, practices, and aesthetics that no longer conform to the “Latin American art” category. It is thus essential to highlight the multiple artistic initiatives that are allowing them to gain visibility and recognition within both the local and global artistic milieus. We posit that today it is almost impossible to overlook both the historical and the ongoing presence of Latin American art and artists in Canada and the recent emergence of a vibrant, ever-expanding contemporary Latinx Canadian art scene. This section proposes six groundbreaking contributions that, from coast to coast, offer further data and analysis, case studies, and investigations into museum archives: from Vancouver to Montréal, from pre-Columbian art and material culture to contemporary art, from the Chilean diaspora of the 1970s to more recent migration waves, from curatorial strategies to the classroom.
Introduction to the Dialogues on Latin American Art(ists) from/in Canada
Authors: Alvarez Hernandez Analays , Robin Alena
Published: February 2022

This Dialogues section seeks to contribute to the scholarship on Latin American art in Canada and “Latinx Canadian art.” We aim to broaden the historical and current narratives of art and artists from Latin America north of the United States, taking into account Canada’s history of migration and its official bilingual status (French-English), multilingual and multicultural reality, and relationship with Indigenous peoples. Adding to the urgency of studying the presence of Latin American art in Canada, there is also a need to focus on the work of artists and curators with a Latin American background. They are developing languages of expression, practices, and aesthetics that no longer conform to the “Latin American art” category. It is thus essential to highlight the multiple artistic initiatives that are allowing them to gain visibility and recognition within both the local and global artistic milieus. We posit that today it is almost impossible to overlook both the historical and the ongoing presence of Latin American art and artists in Canada and the recent emergence of a vibrant, ever-expanding contemporary Latinx Canadian art scene. This section proposes six groundbreaking contributions that, from coast to coast, offer further data and analysis, case studies, and investigations into museum archives: from Vancouver to Montréal, from pre-Columbian art and material culture to contemporary art, from the Chilean diaspora of the 1970s to more recent migration waves, from curatorial strategies to the classroom.
Regulation of Tjp1 mRNA by CPEB2 for tight junction assembly in mouse blastocyst
Authors: Jeong Yelin , Lee Seulah , Choi Inchul
Published: February 2022

Cytoplasmic polyadenylation element-binding protein 2 (CPEB2) is an mRNA-binding protein that regulates the cytoplasmic polyadenylation of mRNA and is required for tight junction (TJ) assembly in the trophectoderm epithelium during porcine preimplantation development. However, the regulatory mechanism underlying TJ assembly by CPEB2 has not been examined. The aim of this study was to elucidate how Cpeb2 regulates the subcellular localisation and stabilisation of Tjp1 mRNA for TJ biogenesis during mouse preimplantation. CPEB2 was detected in nuclei during the early stages of development and was localised at apical cell membranes from the morula stage onwards. In the Cpeb2 knockdown (KD), we observed reduced blastocyst formation with impaired TJs, defective inner cell mass development in the blastocyst outgrowth assay, and loss of pregnancy after embryo transfer. More importantly, Tjp1 mRNA was localised apically in the outer cells of control morulae but not in the Cpeb2 KD embryos, indicating that CPEB2 mediated the translocalisation of Tjp1 mRNA from the nuclei. Finally, in the control embryos, the length of the Tjp1 mRNA poly (A) tail was varied, while only a single peak was detected in the Cpeb2 KD embryos. These findings suggest that the binding of CPEB2 to the cytoplasmic polyadenylation element in the 3'-UTR can confer stability on Tjp1 mRNA and translational regulation. In summary, we demonstrated for the first time that CPEB2 mediates Tjp1 mRNA stabilisation and subcellular localisation for TJ assembly during mouse blastocyst formation.
The Influence of Left Ventricular Dysfunction on The Early and Midterm Outcome in The Patients Undergoing Off Pump Coronary Artery Bypass Grafting Assessed by Propensity Matched Score Analysis
Authors: SHIRASAKA TOMONORI , Arayawudhikul Nuttapon , Tantraworasin Apichat , Chartrungsan Angsu , Sakboon Boonsap , Cheewinmethasiri Jaroen , Kamiya Hiroyuki
Published: February 2022

Background: It still remains unclear the depth of influence of left ventricular dysfunction on the recovery of patients’ physical conditions in the early and midterm period following off-pump coronary artery bypass grafting (OPCAB).
Methods: From April 2011 to May 2018, 851 patients underwent OPCAB in our center. All were grouped into two groups: Those whose ejection fraction (EF) was under 35% were defined as the Low EF group (N = 158) and those who maintained EF over 35% were defined as the Faired EF group (N = 693). Preoperatively, there was significant difference in NYHA class (P < 0.001), CCS class (P = 0.038), level of creatinine (P < 0.001), and rate of establishment of IABP (P < 0.001).
Results: Regarding all-cause death in the early postoperative period, low EF was a not a risk factor in patients (P = 0.52) or in the matched cohort (P = 0.398); however, in the midterm, it was a significant risk factor in patients (HR 2.07, P = 0.016) and in the matched cohort (HR 2.72, P = 0.029). Overall survival at 5 years in the Low EF group was significantly inferior to that of the Faired EF group in all (67.4±4.1% and 86.1±2.9%, P = 0.001) and in the matched cohort (66.5±6.4% vs. 86.5±4.5%, P = 0.008).
Conclusion: OPCAB seems beneficial for patients with LV dysfunction considering the early outcome, however, low EF is a significant risk factor for overall death in the midterm period.
Diatom of the Bacillariophyceae Class in Thermophilic Microbial Mats Present in Sulphurous Hot Springs and their Possible Biotechnological Application
Authors: Puy-Alquiza María Jesús , Luna Berenice Noriega , Miranda-Aviles Raúl , Hernández Ma. Mercedes Salazar , Aguilera Gilberto Carreño
Published: February 2022

The diversity of diatoms in the hot springs of the Comanjilla geothermal zone in northern Guanajuato, Mexico was studied. Hot springs are extreme ecosystems that, despite having high temperatures, constitute an environment for many thermophilic microorganisms (bacteria, cyanobacteria, and diatoms). The thermal water studied is classified as hyperthermal water (45°C to 100°C), of deep origin, and with low mineralization, are of type sulfuric sodium chloride, since the dissolved content of hydrogen sulfide (H2S) is found in concentrations higher than 1mg/L, and its smell is similar to the one of rotten eggs, presents a pH of 7.6 to 9.1 that represents neutrophilic to alkaliphilic environments, with a variable electrical conductivity (EC) (658-698 µs / cm) and total dissolved solids (TDS) (314-24 ppm). In the same way, these hot springs present microbial mats that consist of several stratified layers of green and orange color of 100 cm2, each one, which are dominated by specific types of microorganisms such as bacteria, cyanobacteria, but mainly diatoms, the latter were studied applying the scanning electron microscope and the optical microscope. The morphological characteristics observed in the optical microscope and in the scanning electron microscopy indicate the presence of diatoms of the Bacillariophyceae class, represented by Sellaphora disjuncta (55%), Achnanthes brevipes var. intermedia (45%). This diatom present in thermophilic microbial mats in the sulphurous geothermal zone of Comanjilla represents: a) the first report of said microorganisms in the study area and Mexico; b) an ecosystem of great interest from the biotechnological and industrial point of view; c) an important taxon in terms of diversity and technology; d) an applications in biofuels, environmental monitoring, wastewater treatment, manufacture of fertilizers, production of secondary metabolites, medical compounds, energy sources and food industry and within nanotechnology. It is important to mention that the physical and chemical characteristics of thermal water such as temperature, pH, dissolved solids, electrical conductivity, hardness, alkalinity and silica concentrations, were the major environmental factors influencing the distribution of diatoms in sulphurous hot springs.
Deep structure, tectonics, evolution and hydrocarbon potential of the north-western shelf of the Black Sea along 31°20’E
Authors: KOZLENKO M.V. , KOZLENKO Yu.V.
Published: February 2022

A density modeling of the north-western shelf of the Black Sea along 31°20’ E was carried out. According to its results, a complex block structure of the area is determined, which is closely connected with the history of its development. Signs of Baikal tectonic activation of the southern edge of the pre-Riphean Eastern European platform, as well as the adjacent part of the Scythian plate have been revealed. Areas formed during the Hercynian and Cimmerian epochs of tectogenesis have been identified within the Scythian plate. At the base of the Karkinit Trough, two areas of reduced crust resulting from riftogenesis with varying degrees of intensity of basification have been established. Two ancient volcanos of ryolite composition were found on the basement surface on the northern slope of the Kalamit swell. It is highly probable that the Gamburtsev uplift is an eastern extension of the Gubkin swell. According to the structure and value of the calculated densities, it is established that the Gamburtsev uplift is a «blind» mud volcano, which was formed during the second stage of the late Cimmerian cycle and was active throughout the Cretaceous period. A detailed analysis of the deep structure and fault tectonics of the local structures of the sedimentary cover in the intersection of the profile and the area of gas seeps was carried out. It was found that the Flangova, Partizanska and Hamburtseva structures are more promising for hydrocarbon accumulation. The hydrocarbon potential of the Ushakov structure (H-41) is questionable, because structurally and tectonically it is analogous to the Delphin structure, which was deemed unproductive based on drilling results. The area of gas seeps was found to be confined to a mantle fault, which separates two blocks with distinctly different structures and Meso-Cenozoic evolution of the Earth’s crust. It is proposed to conduct a detailed seismic survey in this area in order to identify local structures in the sedimentary cover, promising for hydrocarbons.
Analytical Methods Validation of Retinoic Acid and Hydroquinone Using Ultra High Performance Liquid Chromatography in Medicinal Cream
Authors: Mazroatul Linda , Ulya Annisa Ananda , Supandi Supandi , Mansur Umar
Published: February 2022

Hydroquinone and retinoic acid have been widely used as whitening agents in cosmetic creams and have a negative impact if used without a medical prescription. In this study, development of analytical method of retinoic acid (REA) and hydroquinone (HYQ) were carried out using Ultra High Performance Liquid Chromatography (UHPLC) with a PDA detector, Phenomenex Luna® ODS / C18 column (150 mmx4.6 mm), methanol-acetic acid 0.1% (85:15) as mobile phase, flow rate of 1.0 mL/min and detected at 295 nm for HYQ and 341 nm for REA. The result of the validation method showed a retention time of HYQ at 1.55 minutes and REA at 3.73 minutes. Validation parameters performed are selectivity, range, linearity, LOD, LOQ, accuracy, and precision. Linear detector response in the concentration range of 75-250 ppm with a calibration curve equation. The calibration curve equation for HYQ is y = 0.4607x + 1.9014 (r = 0.999), mean while REA is y = 2.3138x + 21.0429 (r = 0.999). LOD and LOQ values of HYQ are 1.615 ppm; 13.98 ppm respectively and REA 1.02 ppm and 3.11 ppm, respectively. The precision (% RSD) ≤ ± 2% and accuracy (% recovery) in the range of 99.0% - 100.6%. This method is valid to be used to analyze HYQ and REA in commercial cosmetic cream samples.
Uji Efek Imunomodulator Ekstrak Biji Karika (Carica pubescens Lenne K. Koch) terhadap Peningkatan Aktivitas Fagositosis pada Mencit Putih Swiss Webster
Authors: Luhurningtyas Fania Putri , Dyahariesti Niken , M Septi Fitri Eka
Published: February 2022

Tumbuhan dari keluarga Caricae telah diketahui mempunyai aktivitas sebagai antelmintik, antihiperkolesterol, antibakteri, antioksidan, dan aktivitas farmakologis lain yang berhubungan dengan sistem imun. Tumbuhan karika merupakan salah satu keluarga Caricaceae dan  belum banyak diteliti. Kandungan flavonoidnya diketahui lebih tinggi dibandingkan buah pepaya. Penelitian ini bertujuan untuk menskrining aktivitas imunomodulator ekstrak biji karika berdasarkan konstanta fagositosisnya menggunakan metode bersihan karbon. Maserasi dengan pelarut etanol 96% digunakan untuk menarik metabolit sekunder biji karika. Penelitian dilakukan dengan membagi 5 kelompok perlakuan pada hewan uji yaitu kelompok positif Pyllanthus niruri, kelompok negative, serta kelompok perlakuan ekstrak biji karika (EBK) dosis 125, 250, dan 500 mg/kgBB. Hasil penelitian menunjukkan terdapat pengaruh pemberian variasi dosis EBK terhadap kecepatan eliminasi tinta karbon. Biji karika dosis 500 mg/kgBB mempunyai aktivitas sebanding dengan kontrol positif Pyllanthus niruri (p<0,05) sebagai imunostimulan.
Kejadian Luar Biasa COVID-19, Sebuah Tinjauan Literatur Secara Singkat
Authors: Zilhadia Zilhadia
Published: February 2022

Abstract: The world of health is totally horrendous with a disease that attacks internal breathing and can cause death. The cause of this disease was identified as Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) and the desease called Corona Virus Desease 2019 (COVID-19). For the first time in the last two decades, a disease has an impact on the ideology, politics, economics, social, culture, psychology, defense and security of a country, and occurs globally. COVID-19 is a zoonotic disease with a mortality rate of 6,9%. Person-to-person transmission can occur through droplets or direct contact. COVID-19 will be transmitted massively if infection control is delayed or there is no adequate personal protective equipment for health workers. Confirmation of patients who are positive for COVID-19 requires a relatively long time because it requires a careful examination of RT-PCR with a limited number of devices in Indonesia. At present, there is no definitive treatment for COVID-19 even though several drugs are being investigated. To immediately identify patients and prevent further spread, doctors must be aware of travel or contact history with patients who have symptoms of COVID-19. Abstrak: Dunia tiba tiba dihebohkan dengan adanya sebuah penyakit yang menyerang  pernafasan bagian dalam dan dapat menyebabkan kematian. Penyakit ini diberi nama Corona Virus Desease 2019 (COVID-19) yang disebabkan oleh  Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2). Untuk pertama kalinya sepanjang 2 dekade terakhir, sebuah penyakit berdampak terhadao  ideologi, politik, ekonomi, social, budaya,  psikologi, pertahanan dan keamanan suatu negara, dan terjadi secara global. COVID-19 adalah  penyakit zoonosis dengan tingkat kematian mencapai 6,9%. Penularan dari orang ke orang dapat terjadi melalui droplet atau kontak langsung. COVID-19 akan menular secara masif jika pengendalian infeksi  terlambat atau tidak ada alat pelindung diri yang memadai bagi petugas kesehatan. Konfirmasi pasien yang positif terkena COVID-19 membutuhkan waktu yang relatif lama karena  memerlukan pemeriksaan yang teliti yaitu RT-PCR dengan jumlah alat yang terbatas di Indonesia. Saat ini, tidak ada pengobatan yang pasti untuk COVID-19 meskipun beberapa obat sedang diselidiki. Untuk segera mengidentifikasi pasien dan mencegah penyebaran lebih lanjut, dokter harus mewaspadai perjalanan atau riwayat kontak dengan pasien yang mempunyai gejala COVID-19.
Isolasi dan Karakterisasi Bakteri Asam Laktat dari Kefir Susu Kambing Saanen (Capra aegagrus Hircus)
Authors: Rumaisha Ronanda , Aldrat Hendri , Betha Ofa Suzanthi
Published: February 2022

Kefir merupakan minuman kesehatan yang memiliki efek positif bagi manusia yang bahannya bisa berasal dari susu kambing dan sapi. Perubahan susu menjadi masam pada kefir terjadi karena adanya berbagai jenis mikroba yang hidup bersimbiosis pada bulir kefir. Sejauh ini belum banyak dilaporkan jenis BAL pada kefir yang diperoleh dari susu kambing Saanen di Indonesia. Penelitian ini bertujuan untuk mengetahui jenis dan karakteristik BAL yang terkandung dalam kefir susu kambing Saanen. BAL diisolasi dengan menggunakan media MRS yang disuplemen dengan CaCO3 1% dan diinkubasi 48 jam suhu 37oC. Selanjutnya dilakukan karakterisasi BAL dengan cara mengamati morfologi koloni dan sel, karakterisasi fisiologi dan biokimia yang di antaranya: uji katalase, motilitas, tipe fermentasi, pertumbuhan pada suhu 11oC, 37oC, dan 45oC, serta uji pertumbuhan yang disuplemen dengan NaCl 5%, 6.5%, dan 10%. Penelitian ini berhasil memperoleh 4 isolat yang sesuai dengan karakteristik umum BAL, yaitu Gram positif berbentuk bulat, tidak membentuk spora, dan bersifat katalase negatif. Semua isolat bersifat non motil, homofermentatif, dapat tumbuh pada suhu 11oC-45oC dan konsentrasi NaCl 5%-10%. Hasil karakterisasi menunjukkan keempat isolat termasuk dalam genus Enterococcus. Peran Enterococcus sebagai penghasil asam laktat didiskusikan dalam kajian ini.
Kajian Metode Peningkat Kepatuhan Pada Pasien Hipertensi: Telaah Literatur Sistematis
Authors: Novitri Suci Ahda , Saibi Yardi , Muhtaromah Maulia
Published: February 2022

: Prevalensi penderita hipertensi terus meningkat secara tajam. WHO memprediksi bahwa  pada tahun 2025 orang dewasa di dunia akan menderita hipertensi sekitar 29% dari total penduduk dunia. Salah satu penyebabnya adalah karena ketidakpatuhan pasien hipertensi dalam menjalankan terapinya. Penelitian ini bertujuan untuk mengkaji artikel terkait metode yang dapat digunakan untuk meningkatkan kepatuhan pasien hipertensi serta pengaruhnya terhadap kepatuhan. Penelitian ini menggunakan metode yang terstruktur dan sistematis (Systematic Literature Review). Pencarian artikel dilakukan melalui basis data Pubmed, Science Direct, Google Scholar dan diperoleh sebanyak 22 artikel. Berdasarkan hasil kajian artikel menunjukkan bahwa metode yang dapat digunakan untuk meningkatkan kepatuhan pasien hipertensi yaitu konseling, home care, fokus group diskusi (FGD), video, pemberian  leaflet atau brosur edukasi, pemberian pesan singkat (SMS), kotak obat digital, kartu pil (pill box), mobile health dan gabungan beberapa metode. Metode-metode tersebut memiliki pengaruh dalam meningkatkan kepatuhan pasien hipertensi.
Association of Maternal Tdap Recommendations With Pertussis Hospitalizations of Young Infants
Authors: Kim Grace , Berry Jay G. , Janes Jessica L. , Perez Abe , Hall Matt
Published: February 2022

BACKGROUND

It is well established that young infants have the highest risk of severe pertussis, which often results in hospitalization. Since the 2012 recommendation of administering tetanus toxoid, diphtheria toxoid, and acellular pertussis (Tdap) vaccine for every pregnancy, evaluation of pertussis hospitalizations among young infants in the United States has been limited.

METHODS

In this ecological study, we used the Kids’ Inpatient Database, the largest all-payer pediatric inpatient database in the United States, to study pertussis hospitalizations among infants <1 month of age from 2000 to 2016.

RESULTS

The overall rate of pertussis hospitalizations before the Tdap vaccination recommendation was 5.06 per 100 000 infants (95% confidence interval, 4.36–5.76) and 2.15 per 100 000 infants (95% confidence interval, 1.49–2.81) afterward.

CONCLUSIONS

This study supports maternal vaccination against pertussis as an important strategy in protecting young infants, and continued evaluation is needed to assess the long-term trends in hospitalization.
Decay Width of 3-3-1 Model Charged Higgs and Gauge Bosons
Authors: Ramos Kelvin J. , Morgan Carlos A. , Cieza Montalvo Jorge E. , Rivasplata Antonio I. , Ramírez Guillermo H. , Rodríguez Benites Carlos E.
Published: February 2022

The total decay widths of the charged Higgs (H±2) and gauge bosons (V±) have been calculated in the version of the 3-3-1 Model with heavy leptons. In each case, we analyze the decay rates and determine the most likely channels to occur in order to identify the most relevant final events.
Notas sobre Familias y Pandemia. La restauración conservadora de la familia tradicional.
Authors: De Martino Bermúdez Mónica Solange
Published: February 2022

En la época de consolidación del capitalismo la familia fue analizada como el eslabón responsable de garantizar el nexo entre individuo y sociedad, evitando situaciones de anomia. Este artículo -basado en entrevistas concedidas a un reconocido diario de Chile- muestra que en la situación de pandemia, que cataliza una crisis económica internacional y acelera las transformaciones en el mundo del trabajo, pareciera que se pide a la familia que se haga cargo de todo. Propaganda y política subrayan este familismo sanitario, el cual debería reconocer que la familia no siempre es un contexto de recogimiento y placidez, y que es necesario tender una mano a aquellos integrantes que sufren situaciones de abuso, soledad y desborde emocional.
Assessment of selected combined sewer system in the city of Trnava
Authors: Csóka Marek , Marko Ivana , Rózsa Gergely , Stanko Štefan
Published: February 2022

The goal of this work is the hydraulic capacity assessment of selected combined sewer systems in the city of Trnava. The selected sewer system constitutes from 160 sections 3.3 km long. Hydraulic capacity assessment will test sewer system, created in SeWaCAD program with design rainfall events with varied frequencies. The result of assessment showed that hydraulic capacity of combined sewer system did not meet requirements to properly work. Malfunctioning sewer pipelines must be changed. Design rain with frequency at least one in two years will be used as restoration model.
Direito humano à vida: a objeção médica e a interrupção de gravidez
Authors: Nistler Regiane , Ghisi Machado Marco Augusto
Published: February 2022

Este estudo objetivou especificamente estudar o direito à vida em relação a objeção médica e a possibilidade da mulher interromper a própria gravidez. O trabalho é dividido em três seções. A primeira busca abordar o direito à vida, enquanto o segundo visa estudar a objeção da consciência na atuação médica e a terceira a interrupção da gravidez pela mulher, momento no qual o estudo, ainda que brevemente, busca analisar a situação objeção da consciência médica X interrupção da gravidez, tendo como norte e pano de fundo o maior bem: a vida, enquanto direito humano e fundamental. O método de abordagem utilizado neste trabalho será o dedutivo. Tal método almeja explicar o conteúdo das premissas, utilizando-se, para tanto, de uma linha de raciocínio em ordem descendente, que parte da análise do geral ao particular para chegar a uma conclusão.
O Direito à Privacidade e os novos desafios da era da informação no século XXI
Authors: Fredes Andrei , Borges Mariana
Published: February 2022

A privacidade na era da informação é o tema central deste trabalho, especialmente a partir de sua vulnerabilidade percebida nos meios digitais, o que invoca a necessidade de um aprofundamento dessa garantia fundamental. O método de compreensão do Direito adotado é o fenomenológico, especialmente a hermenêutica filosófica, sendo assim, o artigo se divide em três momentos; o primeiro delimita a tradição na qual se constrói o direito à privacidade como garantia fundamental; o segundo momento apresenta a sociedade atual globalizada e difusa como antítese; finalmente, o terceiro momento busca a subsunção da tradição e da tecnologia em uma síntese capaz de responder os anseios sociais frente aos novos desafios propostos, especialmente o do perfilamento (profiling) massivo e de suas aplicações.
Teoria do adimplemento substancial e sua aplicabilidade pela Corte Goiana entre os anos de 2016 e 2019 na perspectiva comparatista
Authors: Domiciano Felipe Aquino
Published: February 2022

A teoria do adimplemento substancial possui previsão doutrinária e jurisprudencial, podendo ser conceituada como empecilho a resolução contratual nas hipóteses em que há ínfimo descumprimento da obrigação pactuada entre os contratantes. Para aplicação da teoria é necessário o preenchimento de três requisitos. O objetivo do presente artigo é analisar um de seus requisitos, qual seja, o inadimplemento ínfimo, sob a perspectiva comparatista entre o Tribunal de Justiça do Estado de Goiás – TJGO e os principais tribunais do país. Através de pesquisa documental e bibliográfica, foram analisados 371 acórdãos proferidos pelo TJGO entre os anos de 2016 e 2019 sobre a temática, dos quais 247 se enquadraram nos critérios de inclusão e fizeram parte integrante dos resultados do presente artigo. Os resultados revelam que o TJGO aplica a referida teoria quando há um adimplemento mínimo de 80% da obrigação pactuada, ao comparar com os principais tribunais do país é possível verificar uma variação de 14%, o que pode acarretar em disparidade de decisões e insegurança jurídica acerca da aplicabilidade da teoria. Como conclusão, é sugerido que o STJ intervenha e estabeleça um percentual que possa ser seguido pelos tribunais em tempos de pandemia da Covid-19.
A Uniformização Jurisprudencial no Novo Código de Processo Civil e a sua (in)compatibilidade com a processualidade democrática
Authors: Miguel Luciano Costa , Monteiro Bruno Costa
Published: February 2022

Considerando o movimento de democratização desencadeado pela Constituição de 1988, orientado pela adoção do paradigma do Estado Democrático de Direito, o presente trabalho pretende investigar se o Código de Processo Civil de 2015 alinha-se à matriz democrática constitucional, permitindo implementação das garantias fundamentais por meio da abertura do processo à participação ativa e efetiva dos cidadãos no procedimento de elaboração de decisões públicas, pelo exercício da liberdade de enunciação de sentidos e a necessária vinculatividade de tais sentidos (efetiva apreciação dos argumentos apresentados pelas partes). Inicia-se, assim, indagando a relevância do Código de Processo Civil para o exercício e implementação de direitos, considerando a sua Exposição de Motivos do Código. Adentra-se no exame dos mecanismos de uniformização jurisprudencial propostos pelo novo Código, traçando alguns paralelos distintivos entre os sistemas jurídico do civil law (romano-germânico) e do common law (anglo-saxão), bem como das técnicas de aplicação dos precedentes e jurisprudência em cada qual. Na sequência, são analisados os procedimentos e dispositivos que o novo Código introduziu para implementar a uniformização de jurisprudência, sempre realizando um cotejo destes com os requisitos institutivos da processualidade democrática. Ao final, busca-se responder se o novo Código de Processo Civil é hábil a assegurar a processualidade democrática, considerando o dever de fundamentação das decisões (art. 489) e o princípio da não surpresa (art. 10), bem como os princípios do contraditório, ampla defesa e isonomia.
A prática de Exposed na internet e a configuração da conduta como abuso de direito
Authors: Fiorentin Driane , Bressan Francielli , Bolesina Iuri
Published: February 2022

O presente estudo objetiva investigar se a prática de Exposed na Internet configura juridicamente violação de direitos alheios, partindo dos estudos acerca do direito constitucional à livre manifestação. Nesse sentido, utilizou-se de uma metodologia de abordagem indutiva, combinado com o método de procedimento monográfico e a técnica de pesquisa em documentação indireta, construindo a pesquisa em 03 momentos: Inicialmente, busca-se conceituar o sentido da palavra “Exposed” e sua aplicação prática nos meios digitais; em segundo lugar, estrutura-se os conceitos doutrinários acerca do direito à livre manifestação e a caracterização do abuso de direito, sendo que por fim, objetiva-se entender quais práticas de Exposed configuram abuso de direito. Dessa forma, este trabalho foi desenvolvido com o objetivo de compreender as diferentes formas da prática do Exposed e sua possível configuração como abuso de direito. Concluiu-se que é possível exercitar esta conduta sem incorrer em abuso de direito, resguardando informações íntimas acerca do autor dos fatos, mantendo o foco na descrição do ocorrido e permitindo a ocorrência de diálogo nas redes sociais.
MARCADORES DE DANO OXIDATIVO NA DOENÇA DE FABRY
Authors: Souza Marinês Ghizi , Marinho Jéssica Pereira , Teixeira Isabel Cristina Proença , Lima John Thomas Weriang , Abreu Tamires Marques , Silva Luiz Fernando Lopes , Funchal Claudia , Dani Caroline , De Mello Alexandre Silva
Published: February 2022

A doença de Fabry (DF) é uma doença de herança genética ligada ao cromossomo X, com mutações no gene que codifica a enzima lisossômica Alfa-galactosidase A (α-gal A). A baixa atividade enzimática da α-gal A, resulta no acúmulo progressivo do substrato Globotriaosilceramida (Gb3) nas células, prejudicando a função do sistema metabólico e podem estar associado a alteração das espécies reativas de oxigênio e do sistema de defesa antioxidante que contribue para o estresse oxidativo. O objetivo do estudo foi testar, através da análise de amostras de plasma, avaliando a peroxidação lipídica analisada por substâncias reativas ao ácido tiobarbitúrico (TBARS), dano protéico por ensaio de carbonila, defesas antioxidantes não enzimáticas pelo teor de sulfidrila (SH), defesas enzimáticas antioxidantes por  superóxido dismutase (SOD) e catalase (CAT),. Analisamos dois grupos, sendo um com 14 pacientes diagnosticados com DF que não receberam tratamento prévio, comparados com 13 individuos do grupo controle. . A atividade da enzima α-gal A  foi medida no plasma e leucócitos. Os resultados no grupo da SOD e CAT apresentou uma correlação positiva entre os dois grupo. Porem não mostraram diferenças significativas (p  0,05) nos níveis de TBARS, carbonila, sulfidrila e SOD. Em conclusão, os parametros estudados manteram uma constante nos níveis de defesa enzimática e não enzimática, sem nenhum impacto sobre a peroxidação lipídica e danos às proteínas. Consideramos que outros fatores no plasma de paciente com Fabry  podem contribuir para o extresse oxidativo.
Avaliação do perfil lípidico e renal de homens em treinamento de hipertrofia miofibrilar
Authors: Brolo Kelly Carolina De Quadros , Júnior Sérgio Coitinho , Gaudenzi Rachel , Ribeiro Jerri Luiz , Jacobsen Marcia , Cardoso Valesca Veiga , Mascarenhas Marcello
Published: February 2022

Trata-se de um estudo transversal, que teve como objetivo de analisar possíveis alterações no colesterol total, HDL-c, triglicerídeos, ureia e creatinina, a partir de informações secundárias de um banco de dados de um projeto de pesquisa sobre os biomarcadores lipídicos e renais de homens em treinamento de hipertrofia miofibrilar de uma academia situada em Porto Alegre, que foram divididos em dois grupos: grupo teste que recebeu cápsulas contendo vitamina C, vitamina D3, zinco e Serenoa repens, e grupo controle que recebeu cápsulas de amido demilho. Ambos os grupos administraram as cápsulas por via oral, de 12 em 12 horas. As dosagens bioquímicas foram realizadas no soro dos participantes durante dois momentos: antes dos participantes iniciarem a suplementação e após 45 dias de uso. As determinações bioquímicas seguiram as recomendações conforme o método enzimático colorimétrico da LABTEST®. Os resultados mostraram que pós a intervenção o grupo teste demonstrou um aumento significativo do colesterol total (p=0,048) e HDL-c(0,055) e diminuição nos níveis de triglicerídeos (p=0,030) quando comparado ao controle. O grupo teste apresentou uma diminuição nas concentrações de creatinina (p=0,527) e ureia (p=0,044) quando comparado ao controle.
PREVALÊNCIADE TUBERCULOSE PULMONAR NO BRASIL: UMA REVISÃO BIBLIOGRÁFICA
Authors: Pacheco Laís Da Silva , Jacociunas Laura Vicedo
Published: February 2022

A tuberculose é uma doença infectocontagiosa que afeta frequentemente os pulmões, cujo agente causador é o Mycobacterium tuberculosis. É transmitida pela fala, tosse ou espirro de um portador bacilífero. Possui bom prognóstico e cura quando diagnosticada e tratada precocemente. Segundo a Organização Mundial da Saúde, cerca de um quarto da população mundial possui infecção latente por este microrganismo. Está entre as dez principais causas de morte no mundo e a principal causa por um único agente transmissor. O estudo teve como objetivo realizar uma revisão de literatura sobre a prevalência de tuberculose no Brasil. Adotou como metodologia e ferramenta embasadora, a utilização de materiais já publicados, livros, artigos científicos, publicações periódicas e documentos oficiais. Trabalhos que constavam na lista de referências dos artigos encontrados foram utilizados como leitura suplementar. Os termos utilizados para busca foram: bacilo de Koch, cultura, diagnóstico, prevalência, microbactérias, Mycobacterium tuberculosis, radiografia de tórax, teste rápido molecular,tuberculose pulmonar, tratamento, bem como a combinação entre eles nos idiomas: português, inglês e espanhol. Não houve limite de ano para busca de publicação. Os artigos foram selecionados pelo título e resumos. Apresenta como resultados aumento na média de prevalência nacional em 2019, seguido por queda acentuada em 2020. Há um predomínio de acometimento pela doença no sexo masculino, além disso, ela se apresenta mais prevalente nas regiões Norte e Sudeste. Conclui-se que o Brasil apresenta tendência de queda nos índices de tuberculose, mas ainda possui alta prevalência, dentre outros índices relevantes.
Research on the effects of temperature, salinity and nutrition to the growth of the Betaphycus gelatinus (Esper) Doty
Authors: Trung Vo Thanh , Huynh Tran Van , Duc Tran Mai , Nghia Le Trong , Hung Pham Duc
Published: February 2022

Betaphycus gelatinus (Esper) Doty (BG) is a red seaweed that has been used as a food and a research material. Thus, the study on factors affecting the growth and development of BG is necessary. The present results show that the good growth conditions of BG are 27 oC, 30‰ of salinity, and add PES or Nitrate-phosphate medium. This research has important scientific significance in preserving and restoring rare and valuable commercial seaweed varieties.
Expression of a new serpin gene screening from metagenomics database in \(\textit{Pichia pastoris}\) and some characterization of recombinant protein
Authors: Cuong Pham Viet , Hong Tran Thi
Published: February 2022

Currently, searching for new protease inhibitors is of interest to many scientists around the world because they play an important role in controlling harmful proteases. As terrestrial bioactive resources are becoming increasingly depleted, studies are aimed at finding other sources, for example, the ocean. Some recent reports indicate that sponge- associated microbes produce substances with high biological activity, such as anticancer and anti-inflammation, protease inhibition, etc. In this report, a new serpin gene screened from the metagenomics database of microorganism-associated Spheciospongia vesparium QT2 collected sea of Quang Tri province (Vietnam), was successful expressed in Pichia pastoris SMD1168. The obtained result showed that the maximum amount of recombinant protein secreted in the medium was received after 72 hours of induction with methanol. The PAGE electrophoresis supplemented with 0.1% casein and the Trypsin-sepharose 4B affinity chromatography column were performed, and the result was confirmed that the recombinant protein expressed in the P. pastoris SMD1168 (molecular weight about 50 kDa) was the target protein. The purified recombinant protein PI-QT exhibited inhibitor activity on trypsin, ɑ-chymotrypsin and thermolysin and the inhibition was 88.7%, 69% and 43%, respectively. In addition, PI-QT protein is stable and has optimal activity in the pH range 7−9, the temperature is below 60 oC.
Detection of single nucleotide polymorphisms (SNPs) in a SARS-CoV-2 virus strain in Vietnam
Authors: Thach Tran Xuan , Duong Bui Thi Thuy , Truong Le Van , Hoa Nguyen Thi , Lanh Pham Thi , Uy Nguyen Thai , Mai Le Quynh , Anh Dang Duc , Khang Dinh Duy , Quyen Dong Van
Published: February 2022

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of a pandemic of acute respiratory disease in humans. This pandemic has now spread worldwide and caused more than 2,000,000 deaths by 15 January 2021. The complexity and the ongoing pandemic of coronavirus SARS-CoV-2 make it difficult to control the disease. Sequence analysis on some open reading frames (ORFs) of a SARS-CoV-2 virus strain isolated in Vietnam in February 2020 (SARS-CoV-2/NIHE/human/2020/VIE strain) revealed some single nucleotide polymorphisms (SNPs) that only appeared in the Vietnamese strain in comparison to those of the isolates in other countries, including: (1) a change in ORF8-Nucleocapsid (accession number MT127114.1) at nucleotide (nt) 691 (CTC in SARS-CoV-2/NIHE/human/2020/VIE strain, TTC in all other isolates) but it does not change the encoded amino acid, (2) ORF3a-EM-ORF6-ORF7a region (accession number MT127115.1) has four-point changes, three of which lead to changes in the amino acid sequences, being nt 479 (GTA encoding  Valine changed into TTA encoding Leucine), nt 575 (CGC encoding Arginine changed to GGC encoding Glycine) in the M gene, and nt 1126 (GTG encoding Valine changed to GAG encoding Glutamic acid) in ORF6. Taken together, the results provided useful information for the SARS-CoV-2 diagnostic kit and vaccine development.
Identification and evaluation of enzymes gelatinase, amylase, and catalase produced by rice root endophytic bacteria isolated from Hai Duong with antimicrobial properties against \(\textit{Xanthomonas oryzea}\) pv. Oryzea
Authors: Phuong Nguyen Van , Linh Nguyen Thuy , Duy Trinh Dinh , Khanh Tran Le Nam , Giang Do Doan Thu , Linh Ta Thi Thuy , Nga Mai Thi Phuong , Huong To Thi Mai , Chung Mai Duc , Ha Chu Hoang , Binh Le Tran
Published: February 2022

Endophytic bacteria (EB) possess different beneficial traits. Endophytic microbes are often functional in that they may carry nutrients from the soil into plants, modulate plant development, increase stress tolerance of plants, suppress virulence in pathogens, increase disease resistance in plants, and suppress development of competitor plant species. They may enhance plant development by carrying nutrients from the soil into plants and protect plants against phytopathogens by synthesizing extra-/intracellular proteolytic enzymes, as well as releasing antimicrobial metabolites and competing with pathogens for habitation and nutrients. In this study, we investigated the ability to produce exo/endo-enzymes such as gelatinase, amylase, and catalase, and antagonistic activity of 77 EB strains isolated from lowland rice roots grown in Doan Ket commune, Thanh Mien district, Hai Duong province, Vietnam. Out of 77 isolates, 76 (98.71%) showed the ability to liquefy gelatin after 7 days with different rates of hydrolysis. The test of starch hydrolysis revealed 58 (75.33%) isolates that were able to hydrolyze starch. Fifty-one out of 77 isolates (66.24%) were able to produce catalase. The antagonistic activity of rice root endophytic bacteria was determined against bacterial leaf blight disease-causing pathogen Xanthomonas oryzae pv. oryzae (Xoo), strains X19.2 and VX41. We found that three isolates (TP5, TP7, TP11) showed the ability to inhibit the growth of strain VX41 and twelve isolates (TP3, TP7, TP8, TP9, TP10, TP11, TP12, TP13, TP15, TP17, TP21, TP23) were able to inhibit the growth of strain X19.2. These results are served as a venue for further investigation in planta under the conditions of net house and field trials in order to confirm the potential strains for the development of bioinoculant toward controlling the disease caused by Xoo.
O ENSINO DE INTERTEXTUALIDADE NOS ANOS FINAIS DO ENSINO FUNDAMENTAL
Authors: SOUZA-SANTOS JOSÉ ELDERSON DE , ARAÚJO HELENA DE LIMA MARINHO RODRIGUES
Published: February 2022

RESUMO: Objetiva-se discutir possibilidades de trabalhos com a intertextualidade nos anos finais do Ensino Fundamental. Especificamente, busca-se: a) analisar as características de distintos processos intertextuais (plágio, citação, paráfrase, alusão, referência, paródia e pastiche), a fim de associá-las ao ensino da intertextualidade, e b) demonstrar, através de análise em exemplário, diferentes possíveis abordagens desse fenômeno, considerando o público do ciclo de ensino delimitado e as distintas e diversas propriedades de cada processo intertextual considerado. Toma-se, para amparo de tal investigação, Cavalcante (2019), Marcuschi (2008), Marquesi, Pauliukonis e Elias (2017) e Santos e Teixeira (2017) ao se discutir a aproximação entre texto e ensino. Em Genette (2010) e Piègay-Gros (2010), discute-se o conceito de intertextualidade e como esse se desdobra através de processos intertextuais. Cavalcante, Brito e Zavam (2017) são fontes que fundamentam a análise da intertextualidade em prol do ensino, e, em Nobre (2014), é possível compreender como cada processo intertextual se organiza, constitucional e funcionalmente. Em termos metodológicos, trata-se de uma pesquisa qualitativa que, quanto aos seus objetivos, pode ser classificada como exploratória e, quanto aos procedimentos técnicos, bibliográfica. Neste trabalho, ao se fazer dialogar os autores supramencionados, realiza-se discussão teórica a qual propõe o estreitamento da relação intertextualidade e ensino, aplicando, em seguida, tal discussão a exemplário específico, cuja análise contribui para orientar o entendimento da investigação e para se pensar o emprego desta à sala de aula.
TEACHING-SERVICE INTEGRATION IN SUPERVISED INTERNSHIP IN NURSING: THE PERSPECTIVE OF NURSING SUPERVISORS, PROFESSORS AND MANAGERS
Authors: Ramos Tierle Kosloski , Nietsche Elisabeta Albertina , Backes Vânia Marli Schubert , Cogo Silvana Bastos , Salbego Cléton , Antunes Andrei Pompeu
Published: February 2022

ABSTRACT Objective: to analyze the influence of supervised internship on teaching-service integration from the perspective of internship nursing supervisors, faculty advisors and managers. Method: this is a qualitative descriptive-exploratory, carried out in an undergraduate nursing course at a federal university in the countryside of Rio Grande do Sul, between February and July 2018, with 26 participants: managers, faculty advisors, primary care nursing supervisor and hospital care nursing supervisor. The semi-structured interview was the technique used for data collection, which were analyzed using Content Analysis. Results: in the first category, “influences of supervised internship on healthcare services”, the possibility of mutual transformation for the service and for students was verified, promoting professionals’ learning, sharing new ideas and encouraging nurses in the search for knowledge. However, it was identified students’ lack of interest in carrying out activities and limitations in planning joint actions. In the second category, “teaching-service integration: limitations and possibilities”, it was identified that teaching-service integration is occurring in a fruitful way, while advances are still needed for its realization. The planning of internship actions is not always consistent with the healthcare service’s needs and activities that take place in teaching are not always shared with professionals. Internship was described as a potentiator in effecting integration. Conclusions: developing internship enables the realization of learning, mobilizes the routine of services and professionals, instigating them to change and improving knowledge, promoting healthcare qualification.
VALIDATION OF AN EDUCATIONAL BOOKLET: EFFECT ON THE KNOWLEDGE ABOUT PREVENTION OF METABOLIC SYNDROME IN ADOLESCENTS
Authors: Rocha Mariana Rodrigues da , Vale Hiugo Santos do , Moreira Thereza Maria Magalhães , Borges José Wicto Pereira , Machado Ana Larissa Gomes , Silva Ana Roberta Vilarouca da
Published: February 2022

ABSTRACT Objective: to validate an educational booklet for the promotion of knowledge about the prevention of Metabolic Syndrome in adolescents. Method: a time series quasi-experiment conducted in 2020 with 37 adolescents from five public schools belonging to the teaching state network of Picos, Piauí, Brazil. It was conducted in two phases: 1) development of a script of questions about the prevention of MS and its content validation by 17 experts; 2) quasi-experiment with reading of the booklet called “Síndrome Metabólica: Como me prevenir?” (“Metabolic Syndrome: How can I protect myself?”, and assessment of the adolescents' knowledge through a script of questions before and after reading the booklet. To analyze content validity of the script of questions, the Content Validity Ratio was calculated (CVR>0.529). The McNemar test (p<0.05) was used to investigate the effect of the educational booklet on the adolescents' knowledge. The study was approved by the Research Ethics Committee of Universidade Federal do Piauí. Results: the script of question's overall CVR was 0.718, representing good content validation. Three assertions were removed (CVR<0.529). Regarding the adolescents' knowledge, 45.9% improved it, with no significant difference (p=0.067). Conclusion: the adolescents' knowledge was higher in almost half of the participants in the post-test, showing that reading the educational booklet favored knowledge about the prevention of Metabolic Syndrome.