bluuebunny/crossref_metadata_2025 huggingface dataset:
The evaluation of feeds diets and additives by the pig producer
Authors: Fowler V. R.
Published: September 2010

The degree of penetration of advances in nutritional research to the benefit of the pig producer is of considerable concern to both scientists and advisers. In formulating the nutritional policy for his pig enterprise, the farmer relies heavily on the advice which he receives from either national or commercial advisory services, and on his own first-hand experience. There may be other influences affecting his decisions, such as the reputation of a particular product or company, but apart from these it is rare to find farmers who have made a really serious objective attempt to evaluate their whole feeding programme. Recent developments in the economic climate have produced a situation in which profit margins for compounders and producers have reached critical levels; this, combined with world shortages of ‘safe’ protein concentrates such as fish meal, has provided a stimulus for the reassessment of established practices and loyalties. The purpose of this paper is to examine some of the ways which are open to the farmer or his adviser for evaluating in an objective way the possible consequences of changing from one diet or feeding practice to another.
The contribution of science to the improvement of hill sheep production
Authors: Lucas I. A. M.
Published: September 2010

Hill farms vary greatly in size, soil and climate and each has its particular problems. The task of the scientist is to investigate each facet of production from the hill and to provide information which will improve the overall understanding of the potentials and limitations to output. From that understanding, the solutions to the problems of the individual farms may be derived.
Hormonal control of reproduction in cattle
Authors: Lamming G. E. , Hafs H. D. , Manns J. G.
Published: September 2010

In countries with developed agricultural systems the proportion of dairy cattle inseminated artificially increased substantially following the Second World War, but a similar expansion in the use of artificial insemination (AI) has not been experienced for beef herds. The availability of deep frozen semen provided a further incentive to increase the percentage of dairy cattle artificially inseminated, for the more progressive herd owners soon realized that the technique offered the advantage of a long-term breeding programme with semen from progeny-tested sires available at a relatively modest cost. More recently, however, various developments in herd management have tended to militate against the use of AI in dairy herds, and during the past year there has been a substantial decrease in the total number of dairy cows inseminated which can only be partially explained by the smaller decrease in the size of the national herd. Amongst reasons advanced is the increase in average size of individual herds, resulting in more cows per man and therefore less time available for the observations of oestrus. Since accurate detection of the onset of oestrus is so important in obtaining high fertility following AI, some herd owners have returned to using natural mating. Evidence supporting this view can be derived from the fact that during 1973/74 there was an increase of over one thousand in the number of bull licences issued in England and Wales above the seventeen thousand issued in 1972/73. Of the 18.2 thousand bulls licensed, there were 3.2 thousand crossbred bulls, some obviously for use in dairy herds. The availability of synchronized and controlled ovulation would largely remove the difficulties which have been encountered, and would permit planned management techniques to be more widely applied within such herds.
Language practice with multimedia supported web-based grammar revision material
Authors: Baturay Meltem Huri , Daloglu Aysegul , Yildirim Soner
Published: September 2010

The aim of this study was to investigate the perceptions of elementary-level English language learners towards web-based, multimedia-annotated grammar learning. WEBGRAM, a system designed to provide supplementary web-based grammar revision material, uses audio-visual aids to enrich the contextual presentation of grammar and allows learners to revise target grammatical structures using interactive exercises such as gap-filling, combo-box and drag-and-drop exercises. This study gathered quantitative data on learner satisfaction, attention and participation and qualitative data with regard to the material’s content, visual and instructional design, usability, practicality and effectiveness. The findings indicate that learners enjoyed using the material and developed a positive attitude towards the system.
The development of meaningful interactions on a blog used for the learning of English as a Foreign Language
Authors: Rivens Mompean Annick
Published: September 2010

The use of a blog as a pedagogical aid for the learning of foreign languages is gaining support among teachers’ communities (Soubrié, 2006; Tomé, 2007) as it can help students develop online interactions and authentic productions. The current study is analysing the use of such a multimedia setting in a group of Master’s students in France, studying English as a Foreign Language, who had to keep a blog in groups of three or four, dealing with a specific topic of their interest. The introduction of such a tool was meant to motivate learners to practise written expression with an added value: the authenticity of the posted message, aimed not only at the teacher and the learning community but also made visible to the outside world.

The aim of this article is to measure the pedagogical added value of such a blog for the development of written expression more specifically, and to see the potential to transform a real activity which is well-known to the younger learners’ community1 into a learning activity for the learning of English. The aims are in agreement with the principles of the Common European Framework of References for Languages (Council of Europe, 2001), which establishes specific goals for language learners with the implementation of ICT: task-based learning, authentic interactions or collaborative learning (Wenger, 1998).

Three main aspects have been considered in this article:

(i) a description of the way the interactions take place on the blog;

(ii) an analysis of the motivating factors for such a publication online;

(iii) reflection on the role of the tutor and on the place for feedback.

A quantitative analysis of the interactions shows that the project is quite successful in terms of participation, as there are more posts on average than required in the pedagogical contract. Yet there are some disparities, concerning the level of activity from one blog to another and among the participants, that can be related to the role they undertake within the blog (do they prefer to post messages or comments, who are these for and why are they posted?) and to their level of motivation. Qualitatively, the project is also positively perceived: although the blog is not considered as a “real-life” one (the activity is perceived as a pedagogical one), the interactions are meaningful because they make sense for the learners who are fully engaged in the writing process and in the interactions. Finally, the place for feedback needs to be discussed, as the corrections online, although described as necessary, are also very often perceived as inhibiting and appear to be a critical element of the project.
The effect of feeding diets containing intact or partially detoxified lupin on voluntary intake and milk production by Friesian dairy cows
Authors: Mukisira E. A. , Phillip L. E. , Mitaru B. N.
Published: September 2010

The study determined the effects of partial removal of alkaloids (detoxification) in crushed lupin seed (CLS) on voluntary food intake, and yield and composition of milk from dairy cattle. Twenty multiparous Friesian dairy cows (first 90 days of lactation) were assigned, according to a randomized complete-block design, to five diets. The diets were formulated to be isonitrogenous (25·6 g N per kg of diet dry matter (DM)) and contained napier grass, lucerne hay, maize bran and urea. The control diet (CON) contained sunflower meal; two diets contained intact CLS at 150 (LUI-15) or 300 g (LUl-30) per kg diet DM. The other two diets contained detoxified CLS at 150 (LUD-15) or 300 g (LUD-30) per kg diet DM. Lupin seeds were detoxified by treatment with boiling water, followed by steeping in cold water. The diets were analysed by gas chromatography for the alkaloids, lupanine and 13-hydroxylupanine. The total alkaloid content of LUI-15 and LUl-30 was 3·8 and 8·0 g/kg diet DM, respectively; by contrast that of LUD-15 and LUD-30 was 2·1 and 5·2 g/kg diet DM respectively. Increasing the level of intact CLS in the diet led to a decrease in voluntary food intake. Cows given LUl-30 had a lower milk yield (11·1 kg/day) than those given LUI-15 (13·8 kg/day;P< 0·01) but there was no difference in either milk protein yield or content. Detoxification of lupin removed proportionately about 0·40 of the total alkaloids from intact CLS and increased food intake, and the yield of milk and milk protein (P< 0·05) but reduced milk fat content. Detoxification of CLS also reduced the rumen degradability of lupin protein (P< 0·05). It is concluded that the reduction in organic matter intake and milk yield of cows given diets containing intact CLS was due to the presence of lupanine and 13-hydroxylupanine. To maximize its usage in diets for dairy cattle, lupin should be detoxified; it can then be included at levels up to 300 g/kg diet DM.
Effects of post-ruminal fermentation on the faecal and urinary excretion of purines
Authors: Surra J. C. , Guada J. A. , Balcells J. , Castrillo C.
Published: September 2010

The effect of post-ruminal fermentation on faecal output of purine bases (PB) and urinary excretion of their derivatives (PD) was studied by the infusion of yeast RNA (2·9 g/day), corn starch (100 g/day), cellulose (200 g/day) and saline solutions into the gastro-intestinal tract offour sheep (35·4 kg live weight) fitted with caecal and duodenal catheters and given 0-80 kg/day alfalfa hay. All substrates were infused into the caecum except cellulose that was infused into the proximal duodenum.

The infusion of RNA did not affect either the faecal excretion of PB or the urinary excretion of allantoin or total PD, although xanthine excretion increased significantly from <0·01 to 0·12 mmol/day.

Starch and cellulose infusions promoted a significant increase in the faecal excretion of diaminopimelic acid (206 and 159v.131 mg/day) and PB (4·55 and 3·62v.2·29 mmol/day) and modified the partitioning of total nitrogen losses between faeces and urine. The urinary excretion of allantoin and total PD were not affected by the caecal infusion of starch (6·60 and 22·9v.7·02 and 22·8 mmol/day) but both tended to increase with the duodenal infusion of cellulose (7·75 and 26·4 mmol/day).

It is concluded that the urinary excretion ofPD is independent of either the supply of nucleic acids to the caecum or the extent of hind gut fermentation although it may be affected by variations in the flow of undigested fibre along the small intestine.
The effect of energy supply on the contribution of lean tissue to total body protein mass in pigs slaughtered at 100 kg
Authors: Quiniou N. , Noblet J.
Published: September 2010

The effect of energy supply between 45 and 100 kg body weight (BW) on the contribution of lean tissue (muscle plus intermuscular adipose tissue) to total protein mass was studied in Large White castrated males (cLW), crossbred Piétrain × Large White castrated males (cPPx) and boars (bPPx). The pigs were allocated to four energy levels (0·70, 0·80, 0·90 or 1·00 ad libitum) and kept in metabolism cages in experiment 1 or given food ad libitum and kept in individual pens in experiment 2. Daily protein supplies were calculated to be non-limiting for growth and identical for all pigs in experiment 1. Temperature was 23°C in both experiments. The pigs were slaughtered at 100 kg BW and physically dissected; the body tissues were chemically analysed. Taking into account housing conditions, the food intake of pigs in experiment 2 corresponded to 1·20 of ad libitum intake of pigs in experiment 1; data of both experiments were combined. The energy supply and the type of pig influenced significantly the protein content of empty BW (eBW) (170 g/kg on average), of lean (184g/kg on average) and non-lean compartment (eBW minus lean, 152 g/kg on average), the proportion of total protein deposited in lean (604 g/kg of total protein, on average) but not the protein content in fat-free eBW (209 g/kg on average). The fat-free eBW can be predicted as 4·8 times the body protein mass.
Power of different F2schemes for QTL detection in livestock
Authors: Alfonso L. , Haley C. S.
Published: September 2010

The power for detection of quantitative trait loci (QTL) using marker information was compared in several schemes differing in the mating type and the number of parents involved. An experiment based on an F2 population of fixed size obtained by crossing two lines differing phenotypically for a single trait was simulated, assuming that QTLs could be fixed or segregating in the lines crossed. Different additive and dominant QTL effect values and allele frequencies were considered covering a range of different favourable situations for the detection of the QTL. Comparison was done by regression using flanking marker information. Mating animals at the F1generation level minimizing relationships was not worse than mating at random or maximizing relationships. The number of parents used affected the power of the experiment when the QTL was segregating in the original crossed lines. Differences in power were mainly related to the number of males from the original line. When the power of the experiment was high as a result of genetic hypothesis assumed, considering several males increased the power. However, when the genetic hypothesis assumed led there to be less power to detect a QTL, the power was higher when fewer males were used.
Selective grazing by dairy cows in the presence of dung and the defoliation of tall grass dung patches
Authors: Bao J. , Giller P. S. , Stakelum G.
Published: September 2010

Two studies investigated the effect of contaminated pasture on selective grazing, overall grazing behaviour and the process of defoliation of dung patches through experiments targeted at four major questions: (a) how does relative utilization of tall and short grass change as the sward is grazed down? (b) what effect does herbage mass and sward height have on the relative utilization of short and tall grass? (c) how are tall grass patches actually utilized by cattle? and (d) how is overall grazing behaviour influenced by contamination of the sward?

Experiments were conducted in mid to late season using Friesian dairy cattle. In experiment 1, two -pasture types (topped sward (T)v.grazed-only sward (G)) were used. The distribution of bites on tall grass from both pasture types indicated that the grazing animals tended initially to graze short grass when they met a new sward, and then select tall grass as the swards were progressively grazed down. This switch happened earlier in the defoliation process in the topped sward. In experiment 2 observations were conducted on previously grazed and previously ungrazed swards. The distribution of bites on tall grass showed a similar trend to that found in experiment 1 and as the sward was gradually grazed, biting rate significantly declined. There was also a significantly higher total grazing time on the previously ungrazed sward (no contamination by dung). Comparing data based on a consistent biting rate (calculated as the time for 20 consistent bites) and natural biting rate (calculated as the total time for 20 bites) suggested that the grazing animals had increased difficulty in handling tall grass which may explain the declining biting rate as the swards were being grazed down and more bites were directed at tall grass. The defoliation of tall grass dung patches appeared to be concentrated around the edges of the patch. The average area of sward affected by a single dung pat was 1·04 m2 measured at the pre-grazing stage and was markedly reduced to 0·51 m2 at the post-grazing stage. In conclusion, selective grazing is likely to exist due to the presence of dung and conditioned by dung distribution and sward type and this in turn modifies biting rate during grazing down of a sward.
Patterns of oestrus, time of LH release and ovulation and effects of time of artificial insemination in Mediterranean buffalo cows
Authors: Moioli B. M. , Napolitano F. , Puppo S. , Barile V. L. , Terzano G. M. , Borghese A. , Malfatti A. , Catalano A. , Pilla A. M.
Published: September 2010

Thirty-two post-partum Mediterranean river buffalo cows were continuously observed for signs of oestrus from September to December with the aid of two vasectomized bulls. Symptoms of oestrus among female Mediterranean buffaloes are weak, therefore oestrus was assessed based on bull behaviour (following and licking a cow and trying to mount her). Oestrus was divided into three phases based on the bull behaviour assessment. Status of the uterus and ovarian follicles were checked rectally every day for each cow which was detected by a teaser bull to be in oestrus. All cows in oestrus were bred twice by artificial insemination (AI), the first at ovulation and the second (using a different bull) 22 h later.

The average duration of interest shown by the bull towards a cow (from the very first to the last sign of interest) was 68 h and the average length of the three phases was: phase 1 = 21 (s.d. 29) h, phase II = 32 (s.d. 24) h and phase III = 15 (s.d. 15) h. Fifteen buffaloes were bled during oestrus, and LH profiles were determined. No differences were evident among oestruses followed by pregnancy (no. = 18) and the others (no. = 26) for the variables describing behavioural events. Neither intensity of the bull courtship, presence or clarity of mucous discharge, or housing system affected the success of AI. The only differences between pregnant and non-pregnant cows were in the timing between the LH peak and the end of phase II (2·4 v. 14·7 h, P < 0·001), end of phase III (22 v. 40 h, P > 0·05) and ovulation (25 v. 46 h, P < 0·05). Successful pregnancies occurred 34 (s.d. 14) h after the end of phase II. The endocrinology and behavioural patterns of buffalo reproduction need further research to clarify the reasons for non-optimal pregnancy rates after AI.
Vehicle motion and motion sickness in pigs
Authors: Randall J. M. , Bradshaw R. H.
Published: September 2010

Low frequency oscillatory motion (0·05 to 0·5 Hz) experienced in ships and road vehicles is known to cause motion sickness in humans and some predictive models are available. There have been very few studies of the incidence of motion sickness in pigs and none which has attempted to identify the frequencies of motion of transporters which are likely to be implicated. In this study, the vibration and motion characteristics of a commercial pig transporter were measured while seven individually penned 40-kg pigs were transported for short (100 min) journeys and 80-kg pigs penned in groups of 12 or 13 were transported for longer (4·5 h) journeys. Direct behavioural observations were made of individual pigs for symptoms of travel sickness (sniffing, foaming at the mouth, chomping, and retching or vomiting). A comparison was then made between the incidence of travel sickness in pigs and that expected in humans given the measured vehicle vibration characteristics. The low frequencies of motion measured on the transporter (0·01 to 0·2 Hz) were well within the range implicated in human motion sickness with considerable power in the longitudinal and lateral axes but little in the vertical axis. On both short and long journeys pigs exhibited symptoms of travel sickness. The likely incidence of travel sickness on the short journeys predicted by the human model was 24 to 31% which corresponds to approximately two of the seven 40-kg pigs becoming travel sick. The numbers observed were generally lower than this although the same pigs were transported twice each day for 2 days and this may have therefore reflected the effects of habituation. The incidence of travel sickness on the long journeys predicted by the human model was 34%. During these journeys which involved four groups of 80-kg pigs which were not repeatedly transported, 26% of pigs vomited or retched (13 out of 50) while 50% showed advanced symptoms of foaming and chomping. These results are not inconsistent with the human model which should form the basis offurther research.
Evaluation of the spot urine sampling technique to assess urinary purine derivative excretion in lactating dairy cows
Authors: Shingfield K. J. , Offer N. W.
Published: September 2010

The potential of the spot urine sampling technique as an alternative to performing a total urine collection was evaluated. Twelve multiparous Holstein-Friesian cows were given two experimental diets in a complete change-over design using two 14-day experimental periods. Experimental diets were either silage offeredad libitumwith 7 kg fresh weight concentrate supplement as a single meal (SF), or a complete diet formulated from the same ingredients with a similar foragexoncentrate ratio (CD). Total urine collections were performed every 2 h on days 11 and 14 of each experimental period. Subsamples of urine were stored at 20°C and subsequently analysed by high-performace liquid chromatography. Daily allantoin and purine derivative (PD) excretion were highly correlated (r = 0·995, no. = 48,P< 0·001). PD and creatinine excretion during each 2-h interval depended on time of collection (PD,P< 0·001 and creatinine,P< 0·05) and on cow (P< 0·01) but were unaffected by sampling day or treatment. Diurnal variations in the molar ratio ofPD or allantoin to creatinine (PD/c and Ale, respectively) followed similar diurnal patterns as observed for PD and allantoin excretion. The data were used to assess the error of prediction of daily mean PD/c or Ale ratios. Three spot sampling regimens (based on the collection of four 4-h samples, three 8-h samples or two 12-h samples) and also on either single or 2-day urine collections were evaluated. Collection of multiple samples within a day was more reliable than collecting fewer samples over several days. Prediction errors were greater for SF compared with CD. Even the most intensive sampling regimen did not allow an acceptable prediction of daily mean PDIc or Ale ratio, minimum r values for PDIc and Ale ratios were 0·098, 0·136 and 0·547, 0·579 for SF and CD, respectively. Furthermore, daily mean PDIc and Ale ratios proved poor predictors of daily PD and allantoin excretion (r values of 0·69 and 0·72, respectively). Total urine collection appears necessary to assess accurately daily PD excretion in dairy cows.
Genetic parameters and evaluations for somatic cell counts and its relationship with production and type traits in some dairy breeds in the United Kingdom
Authors: Mrode R. A. , Swanson G. J. T. , Winters M. S.
Published: September 2010

Somatic cell count (SCO data collected on individual cows since 1991 were obtained from National Milk Records. Following validation genetic and phenotypic parameters were estimated from the lactation average SCCfor 63 424 Holstein/Friesian (HOD, 7966 Ayrshire (AYR) and 14 509 Jersey (JER) animals. The first three lactations were included in the analyses. The heritabilities (h2) for first lactation log SCC (LSCC) were 0·11 (s.e. 0·01), 0·12 (s.e. 0·02) and 0·09 (s.e. 0·03) for the HOL, JER and AYR breeds respectively. Estimates for herd sire interaction (c2) effects ranged from 0·01 to 0·02. Analysis of the first three lactations with a repeatability model produced similar h2 and c2 estimates. Permanent environment estimates ranged from 0·21 to 0·25. Heritabilities of individual test day LSCC ranged from 0·04 (s.e. 0·02) to 0·10 (s.e. 0·03). Genetic correlations between SCC and milk, fat and protein yield for the HOL breed were 0·06 (s.e. 0·05), 0·14 (s.e. 0·06) and 0·09 (s.e. 0·06) respectively. Genetic evaluations were obtained for 666 595 and 9136 animals for Holsteins and Ayrshires, providing evaluations on 13 525 and 1713 bulls respectively in each breed. The range of LSCC predicted transmitting abilities (PTA) was ±25% although the proportion of bulls with reliability > 0·50 was low at 0·17 and 0-05 for HOL and AYR respectively. The correlations between the LSCC PTAs for HOL bulls with at least 50 daughters and their genetic evaluations for linear type were essentially zero for body and some udder traits. Significant negative correlations were obtained for a number of traits including foot angle (0·14), fore-udder attachment (0·19) and udder depth (0·19) and a positive correlation for teat length (0·15). SCC evaluations will be implemented by the Animal Data Centre. Reliabilities will be lower than production because of the lower h2 for SCC and the lower progeny group size since only 0·80 of recorded cows have SCC records.
The effects of age at slaughter, genotype and finishing system on the biochemical properties, muscle fibre type characteristics and eating quality of bull beef from suckled calves
Authors: Maltin C. A. , Sinclair K. D. , Warriss P. D. , Grant C. M. , Porter A. D. , Delday M. I. , Warkup C. C.
Published: September 2010

Muscle fibre characteristics and biochemical properties of muscle recovered from young bulls of two genotypes (Aberdeen Angus × and Charolais ×), reared on two different diets (silage-based and barley-based) and slaughtered at varying ages between 10 and 19 months of age were established. These analyses were restricted to samples ofm. longissimus lumborum (LI) recovered at 48 h post mortem, vacuum packed and stored at 2°C for 14 days. Biochemical measurements included intramuscular fat content, intramuscular collagen content and its solubility, haem pigment concentration, sarcomere length and myofibril fragmentation. Muscle fibre type was classified according to the contractile nature of thefibres and their metabolic properties.

Intramuscular fat content increased (P < 0·01) with age at slaughter and at a fixed age was greater for Angus × than Charolais × bulls (211·5 v. 295·8 mg/g dry matter, P < 0·01). Total intramuscular collagen and its solubility tended to decrease with age (P < 0·01). Differences in haem pigment concentration in samples of LI were detected between genotype (3·99 v. 3·59 mg/g for Angus × and Charolais × bulls; P × 0·01) and diet (3·97 v. 3·62 mg/g for bulls given barley and silage; P < 0·01), and increased with age at slaughter (P < 0·01). There was a significant increase in eye muscle cross-sectional area with increasing slaughter date (P < 0·01) and this was paralleled by an increase in cross-sectional area of individual muscle fibres (P < 0·001). Differences in cross-sectional area of individual muscle fibres between genotype and diet were small and inconsistent. Charolais × bulls had a greater percentage area of fast twitch glycolytic fibres than Angus × bulls (54·3 v. 49·3%; P < 0·01) and a smaller percentage area of slow twitch oxidative fibres (15·8 v. 18·9%; P < 0·05). Beef tenderness was positively correlated (r = 0·48; P < 0·01) with the frequency of slow twitch oxidative fibres and negatively correlated (r = -0·38; P < 0·05) with the frequency offast twitch glycolytic fibres.
Meat and carcass quality in two pig lines of different stress-susceptibility genotype and their crosses
Authors: de Smet S. , Bloemen H. , van de Voorde G. , Spincemaille G. , Berckmans D.
Published: September 2010

In order to investigate differences in carcass and meat quality between lines of different stress susceptibility, 150 pigs were slaughtered on 15 slaughter days, consisting of equal numbers of stress-susceptible (nn) pigs of a boar line, stress-resistant (NN) pigs of a sow line and stress-carrier (Nn) crosses of these two lines. Carcass quality was determined by a SKGII-device which combined four physical measurements into a carcass lean estimate and a conformation score, and meat quality traits were measured on the carcass and on a loin slice. Killing-out proportion was lower for the NN line compared with the Nn crosses and the nn line. Lean content increased and conformation score improved as the number of n alleles in the respective genotypes increased but the Nn genotype was closer to the nn genotype than to the NNgenotype. Similarly, a large difference in meat quality was found between the NN and the nn line, whereas the difference in meat quality between the Nn and the nn genotype was smaller than expected. This was probably due to the low-voltage stunning procedure. In addition, the method of transporting the pigs to the abattoir (by truck or by foot, for a distance 125 m) had a pronounced effect on meat quality in the loin.
The effect of host diet on the gas production profile of hay and high-temperature dried grass
Authors: Huntington J. A. , Rymer C. , Givens D. I.
Published: September 2010

One of the sources of variation in the in vitro gas production (GP) technique is the inoculum source that is used and this could be greatly affected by the diet that is given to the donor animal. To study the effect of the host diet on the gas production profiles of grass hay and high temperature dried grass, two cows were offered either a silage: barley diet (80:20 dry-matter (DM) basis; GSB) or barley straw (ad libitum; STR). An adaptation period of 3 weeks was used and each animal experienced each diet type twice. Rumen fluid and solids were collected at the end of each 3-week period and used to inoculate the substrate cultures. The volume of gas produced (mllg DM) was 379 and 289 for GSB and STR respectively; gas yield (mllg organic matter degraded) was 442 and 411. The maximum fractional rates of degradation (per h) were 0·067 and 0·061 and the time (h) taken to reach these rates were 5·3 and 12·6. None of these differences was significant. There were also no significant differences in the concentration of total volatile fatty acids (VFA) in the final incubation media, or in the molar proportions of individual VFA. These results suggest that the fermentation stoichiometry was not affected by donor animal diet and, while microbial activity from STR was lower, this did not significantly affect the GP profile. The difference in microbial activity between the two diets was perhaps minimized by taking the sample of rumen contents before the morning meal.
A strategy for QTL detection in half-sib populations
Authors: Koning D. J. de , Visscher P. M. , Knott S. A. , Haley C. S.
Published: September 2010

A statistical analysis strategy for the detection of quantitative trait loci (QTLs) in half-sib populations is outlined. The initial exploratory analysis is a multiple regression of the trait score on a subset of markers to allow a rapid identification of possible chromosomal regions of interest. This is followed by multiple marker interval mapping with regression methods within and across families fitting one or two QTLs. Empirical thresholds are determined by experiment-wise permutation tests for different significance levels and empirical confidence intervals for the QTLs' positions are obtained by bootstrapping methods. For traits with evidence for a significant single-QTL effect, an approximate maximum likelihood analysis is performed to obtain estimates of QTL effect and the probability of the QTL genotype for each parent of a group of half-sibs. The strategy is demonstrated in an analysis of previously published data on chromosome 6 and five production traits from a granddaughter design in dairy cattle. The results confirm and extend evidence for QTLs affecting protein percentage. Informativeness of markers limited the possibility of mapping more than one QTL on the same linkage group.
The effects of altered suckling intensity, boar exposure in lactation and gonadotropins on endocrine changes, fertility and the incidence of lactational oestrus in multiparous sows
Authors: Costa A. N. , Varley M. A.
Published: September 2010

One of the more promising techniques for the improvement of sow productivity has been the use of partial weaning or the separation of the sow and litter for periods of between 3 to 12 h/day towards the end of lactation. The purpose of the present study was to examine changes in steroid hormone concentrations, oestrus and fertility responses in lactating sows subjected to altered sucking, boar exposure and gonadotropins in mid lactation and at weaning. Thirty-two crossbred sows were allocated at random to one of four experimental treatments. Treatment 1 sows (no. = 8) were separated from their respective litters for 3 h/day from 12 days post partum through until weaning which was carried out at 21 days post partum. Treatment 1 sows were also given in-pen boar exposure for 1 h/day between day 12 post partum and weaning. Treatment 2 sows (no. = 8) were given the same protocol of litter separation (LS) and boar exposure (BE) as in treatment 1 and they were also given a combination of gonadotropins (Gn) on day 17 of lactation. Treatment 3 sows (no. = 8) were given LS + BE as above and the sows were injected with Gn on the day of weaning. Treatment 4 sows were controls given no LS, BE or Gn (no. = 8). During lactation, treatment 1 sows exhibited significantly (P < 0·05) reduced progesterone concentrations compared with treatment 2 sows. There were no other significant effects of treatment for any of the periods considered. The separation of sows from their piglets with or without gonadotropic treatment did not result in lactational oestrus in any treated sows. No significant treatment differences were found in the subsequent litter size (piglets born alive or total number of piglets). In conclusion, the present results have demonstrated no significant effects of LS, BE and Gn treatment during lactation on sow and litter performance. This was associated with low levels of peripheral plasma progesterone in all lactating sows.
Genetic analyses of profit for Australian dairy cattle
Authors: Visscher P. M. , Goddard M. E.
Published: September 2010

Direct genetic evaluation of profit was investigated as an alternative to a selection index. PROFk was defined as (net income)/(food requirement) until the start of the kth lactation, for k = 2 to 6. Genetic parameters such as heritabilities and genetic correlations were estimated for profit traits for Australian Holstein-Friesian and jersey dairy cattle. Heritabilities for profit until the start of a given lactation were moderate, ranging from 0·12 (for profit until the start of the second lactation in Holsteins) to 0·31 (profit until the start of the third lactation in Jerseys). Genetic correlations between profit traits were very high, and approached unity for most pairs of traits, so that profit early and late in herd life were nearly the same trait. Genetic correlations between profit traits and stayabilities until a given lactation were high, ranging from 0·71 to 0·97. Genetic correlations between profit traits and first lactation milk yield traits were approximately 0·80 for Holsteins and 0·90 for Jerseys. A single analysis urns carried out for lifetime profit using all data, including cows that were still in the herd at the time of data collection. Heritabilities were 0·13 for Holsteins and 0·19 for Jerseys. Genetic correlations between lifetime profit and first lactation yields were high. For the selection of dairy bulls, a multivariate analysis on a milk yield trait (e.g. protein yield) and profit until the last known lactation of bulls' progeny was suggested.
Comparative feeding value of forages from two cereal-legume based cropping systems for beef production from crossbred (Bos taurus × Bos indicus) steers and subsequent performance of underfed and realimented steers
Authors: Umunna N. N. , Osuji P. O. , Khalili H. , Nsahlai I. V. , Crosse S.
Published: September 2010

Foods produced from two cereal-legume cropping systems were evaluated in a 250-day growth trial using Friesian × Boran crossbred steers. Crops and crop combinations in cropping system 1 were oat-vetch (Avena sativa-Vicia dasycarpa; OV), wheat-trifolium (Triticum aestivum-Trifolium steudneri; WT), teff (Eragrostis tef) and chickpea (Pisum sp). Cropping system 2 differed from 1 in that maize-lablab (Zea mays-Lablab purpureus; ML) replaced ivheat-trifolium. Twenty-four steers (mean initial live weight 157 (s.d. = 4·5) kg were assigned randomly within weight group to four treatments. Treatments comprised ad libitum teff straw (TS) given alone (Tl), TS plus wheat middlings (WM) given at 0·01 M (body weight) (Tl), forages from system 1 fed in the order OV, WT and TS plus chickpea for 135, 48 and 67 days respectively (T3) or forages from system 2 fed in the order OV, ML and TS plus chickpea for 73, 147 and 30 days respectively (T4). The feeding period for each crop ofT3 and T4 was based on its contribution to the total dry matter (DM) yield of the cropping system. Apparent digestibility of DM measured by the acid-insoluble ash method was higher (P < 0·05) for T3 and T4 than for T2. DM intake was higher (V < 0·01) for steers on T2 resulting in 1 kg higher daily intake of digestible DM than for T3 and T4. Steers on T4 gained (average daily gain, ADG) more (P < 0·01) weight than steers on T3 (149 v. 85 g per head per day) but less (P · 0·01) than those on T2 (528 g per head per day). Steers on Tl lost 94 g per head per day. Food efficiency (kg yain per kg food) followed a similar trend as ADG. At the end of the trial, two steers from each treatment were slaughtered for carcass assessment and the remaining steers used in a realimentation study of 120 days and then slaughtered for carcass assessment. Steers which gained poorly during the 250-day trial (Tl, T3 and T4) exhibited compensatory gains of 497, 550 and 565 g per head per day respectively compared with 398 g per head per day for steers on T2. Carcasses from T2 yielded significantly more (P < 0·05) lean and fat than carcasses from T3 and T4. The results suggest that growing food crops in association with legumes has the potential of increasing cattle performance.
A genetic analysis of early growth and ultrasonic measurements in hill sheep
Authors: Conington J. , Bishop S. C. , Waterhouse A. , Simm G.
Published: September 2010

Genetic parameters were estimated for early lamb growth and ultrasonic measurements taken on Scottish Blackface lambs reared under extensive conditions on two Scottish hill farms. Measurements were taken on approximately 2000 lambs born to unselected ewes, and sired by 32 rams previously selected for divergent predicted carcass lean content. Heritabilities for birth weight, marking iveight (at approx. 6 weeks of age) and weaning weight (at 17 weeks) were 0·07 (s.e. 0·04), 0·02 (s.e. 0·03), and 0·14 (s.e. 0·05), respectively. Heritabilities for ultrasonic muscle and fat depths at weaning were 0·27 (s.e. 0·09) and 0·16 (s.e. 0·06), respectively. There was a strong maternal effect on weight which declined from birth with lamb age and was relatively unimportant for the ultrasonic measurements. The rearing environment of the lambs (hill pasturev.‘improved’ (or ‘inbye’) pasture) was an important environmental effect on the heritability estimate for backfat thickness, with that for lambs reared on improved pasture being twice that of hill-reared lambs. The implications of the results from this work on genetic improvement of sheep in liill environments are discussed.
Meat and carcass quality of heavy muscled Belgian slaughter pigs as influenced by halothane sensitivity and breed
Authors: de Smet S. , Pauwels H. , Vervaeke I. , Demeyer D. , de Bie S. , Eeckhout W. , Casteels M.
Published: September 2010

In order to investigate the antagonism between meat quantity and quality, halothane-positive (HP) and -negative (HN) Belgian Landrace pigs (B) and Pietrain × Belgian Landrace crosses (PB) were compared for several carcass and meat quality traits. They originated from three commercial farms and were slaughtered in a commercial abattoir. Carcass quality was determined by a SKGlI-device. Meat quality traits were measured on the warm and cold carcass, and on a loin slice. HP and PB pigs showed better carcass quality, and inferior meat quality, compared with HN and B pigs respectively. No significant halothane sensitivity × breed interaction was apparent for most traits. Sex did not affect meat quality. Slaughter day variance was considerable. The inverse relationship between lean content or conformation score and meat quality was generally more pronounced in the HP group compared to the HN group (except for drip and cooking losses). Irrespective of halothane status, carcass conformational score was more negatively related to meat quality than was lean content.
The effect of winter feeding level on subsequent grazing behaviour and herbage intake of Charolais heifers
Authors: Cazcarra R. Ferrer , Petit M.
Published: September 2010

Two groups of eight Charolais heifers were used to examine the effect of feeding level during the post-weaning winter on the grazing behaviour and herbage intake over three periods during the first 2 months of grazing. The winter diets (L and H) were given to achieve a mean difference of 50 kg between groups at turn-out. The heifers were set-stocked together on a hill pasture where sward height was maintained at 12 cm.

The winter live-weight gains were 0·31 and 0·70 (s.e. 0·032) kg/day for L and H heifers respectively, and spring gains 1·94 and 1·66 (s.e. 0·082) kg/day respectively. Intakes increased throughout the spring grazing for both groups. Absolute intakes were lower for L than for H heifers only during the first period (P < 0·05). Intake per kg live weight was higher in L than in H heifers (23·0 v. 21·1 (s.e. 0·54) g organic matter (OM) per kg live weight). Bite weights of H heifers were constant throughout (434, (s.e. 12·9) mg OM), while bite weights ofL heifers only reached those of H heifers in the last period. Bite weight was linearly related to live weight. Bite rates were not different between groups, but L heifers compensated for lower bite weight by grazing longer and they increased their grazing time after turn-out faster than H heifers. Grazing time was mainly responsible for higher relative intakes in L heifers, and thus, partly, for compensatory gain observed.
Naloxone evokes a nutritionally dependent LH response in post partum beef cows but not in mid-luteal phase maiden heifers
Authors: Sinclair K. D. , Broadbent P. J. , Hutchinson J. S. M.
Published: September 2010

Data from two experiments are reported which test the hypothesis that nutrient and/or dry-matter intake and body condition may interact to modify hypothalamic opioidergic activity and thus influence the pulsatile release of LH during the early post-partum period and during the oestrous cycle. Experiment 1 involved 16 multiparous, twin-suckling beef cows, and was a 2 × 2 × 2 factorial design in which the factors were level of post-partum energy intake (80 v. 130 M) metabolizable energy (ME) per day), the digestible undegradable protein (DUP) content of the post-partum diet (14 v. 31 g/kg dry matter), and treatment with either 200 mg or 400 mg naloxone hydrochloride. Blood samples were collected at 15-min intervals for 4h at weeks 4 and 7 post partum. Naloxone was administered intravenously after the eighth sample. Experiment 2 involved 16 cyclic maiden heifers and was also arranged in a factorial manner, with two levels of body condition at the start of the experimental period (2·50 and 3·16 units) and two levels of energy intake thereafter (40 and 80 MJ ME per day). Seven blood samples were collected at 15-min intervals on 4 days consecutively during the mid-luteal phase of the oestrous cycle. On the first 2 of these 4 days naloxone was administered, whilst on the last 2 days a gonadotropin-releasing hormone agonist (buserelin; GnRH) was administered, both after the fourth sample. Plasma from both experiments was assayed for LH and prolactin (Prl).

In experiment 1, cows on 130 MJ ME per day returned to oestrus and ovulated earlier than cows on 80 MJ ME per day (44·5 v. 55·0 days; s.e.d. = 3·93; P < 0·05). At week 4 post partum the proportional increase in plasma LH following naloxone challenge was greater for cows on 130 MJ ME per day than cows on 80 MJ ME per day (1·38 v. 1·12; P < 0·05), but the converse was true at week 7 (1·15 v. 1·68; P < 0·05). Cows on the high DUP diet required a higher dose of naloxone to elicit an LH response. Few heifers in experiment 2 exhibited an LH response to naloxone. In contrast, there were significant dietary treatment effects on the LH response to GnRH (P < 0·01). Relatively thin heifers on 40 MJ ME per day exhibited the lowest proportional increases in plasma LH to GnRH challenge, whereas heifers on 80 MJ ME per day and given the higher dose of GnRH produced the greatest plasma LH responses. Mean Prl concentrations before and after feeding in experiment 2 were respectively 13·2 and 10·2 ng/l (P < 0·01).

Suckled cows given a high energy diet during the early post-partum period can overcome the opioid mediated block on LH release and resume oestrous cycles earlier than cows given a low energy diet. LH would appear to be inhibited by a non-opioid mechanism in mid-luteal phase heifers. Total pituitary reserves ofLH may be influenced by the animals nutritional status.
Reproductive traits of Angus, Criollo and reciprocal crossbred females in the temperate area of Argentina
Authors: Corva P. M. , Villarreal E. L. , Mezzadra C. A. , Melucci L. M.
Published: September 2010

Reproductive performance of purebred Angus (A) and Criollo (C) cows and crossbred CA and AC cows in the temperate area of Buenos Aires, Argentina, was assessed through the study of reproductive rates, calving date, first insemination date, number of inseminations and weight related variables. The herd was grazed on pastures all year round. Matings were by artificial insemination with visual oestrus detection, from October to December. Heifers first calving was at 3 years of age. All traits were analysed with general linear models including, as corresponded, fixed effects of dam and calf genetic groups, calving year and month, dam physiological status, calving age and first order interactions. Female genetic group adjusted means were used to estimate direct (gl) and maternal (gM) breed differences and heterosis (hl) effects on reproductive traits. Weaning rate was mostly determined by oestrons detection rate. Due to the inhibitory effects of nursing on reproduction, female genetic group effects were significant for suckling cows only: mean oestrous detection rate was higher (P < 0·05) for crossbred cows (AC: 91·2 (s.e. 5-1) %; CA: 88·4 (s.e. 2·9) %) than for A (76·3 (s.e. 2·0) %) and C (49·9 (s.e. 3·3) %); direct breed effects favoured A (glA - g1c = 26·4 (s.e. 3·7) %; P < 0·001) and h1 was 26·7 (s.e. 3·4) % (P < 0·001). When only oestrous-detected cows were considered, direct breed effects were significant only for pregnancy rate fg1A —g1c = 8·5 (s.e. 4·4) %) whereas h1 was significant for pregnancy (8·3 (s.e. 3·8) %), calving (9·3 (s.e. 4·1) %) and weaning (10·1 (s.e. 4·1) %) rates (P < 0·05). Criollo cows calved later than crossbred (≍19 days) and A (≍15 days) cows mainly due to longer post-partum anoestrous. For calving date, direct breed effects favoured A (g1A — g1c = 20·8 (s.e. 5·9) days; P <0·0·001) and h1 was 11·4 (s.e. 2·8) days (P < 0·001). Low reproductive performance in C was not associated with live iveight at the beginning of mating. This genetic group was the latest in showing oestrous behaviour, even when not suckling. Number of inseminations was similar for the four genetic groups. These results sustain the principle of utilizing Fl Criollo-Angus cows to improve beef herd productivity, whereas they do not support the recommendation of using purebred Criollo females under this type of production system.