bluuebunny/crossref_metadata_2025 huggingface dataset:
Perancangan Aplikasi Presensi Dosen Realtime Dengan Metode Global Positioning System (GPS) Dan Location Based Service (LBS) Berbasis Web di Jurusan Teknik Elektronika Politeknik Negeri Sriwijaya
Authors: Aisyah Aisyah , Sari Dewi Permata , Kusumanto RD
Published: March 2024

Absensi merupakan suatu hal yang wajib terutama pada sebuah instansi maupun perusahaan Politeknik Negeri Sriwijaya dalam melakukan absensi masih menggunakan cara manual, sehingga dibutuhkan suatu sistem absensi untuk pegawai yang dapat melakukan pendataan dan pengelolahan data secara efisien serta akurat. Perancangan sistem absensi pegawai ini dilakukan dengan metode pengumpulan data, analisa dan survey serta implementasi sistem dengan mengunakan beberapa pemograman untuk pengelolaan database. Hasil dari perancangan adalah dibuatnya sebuah aplikasi absensi secara real time berbasis web dengan metode GPS dan LBS di jurusan teknik elektronika politeknik negeri sriwijaya. Dengan adanya aplikasi ini diharapkan proses absensi lebih efisien dan bisa dipantau dengan mudah oleh admin maupun pimpinan.
Pemantauan Kangkung di Dalam Showcase Hidroponik Cerdas Menggunakan Teknologi Internet of Things
Authors: Exaudi Kemahyanto , Prasetyo Aditya P P , Sembiring Sarmayanta , Sari Komang Mita
Published: March 2024

Bercocok tanam menggunakan teknik hidroponik memberikan kontribusi baru bagi dunia pertanian terutama dalam penggunaan media air dan tidak membutuhkan lahan yang luas. Teknik ini telah banyak di implementasi sebagian masyarakat umum selain petani untuk budidaya sayuran segar di lingkungan rumahnya. Dengan menerapkan teknologi Internet of Things pada sistem hidroponik, pertumbuhan sayuran yang dibudidaya dapat dengan mudah di pantau dari jarak jauh menggunakan perangkat smartphone. Tujuan dari penelitian ini adalah membangun sistem pemantauan budidaya hidroponik yang diimplementasikan pada purwarupa smart showcase cerdas dan digunakan khusus didalam ruangan. Sistem pemantauan yang dirancang berbasis webserver dan menggunakan platfom IoT Thingsboard untuk visualisasi parameter berbasis grafik. Teknik mengumpulkan data secara online menggunakan google spreedsheet. Thingsboard sebagai user interface untuk memvisualisasikan parameter yang dipantau dalam bentuk grafik. Parameter yang dipantau adalah suhu, kelembapan didalam dan luar showcase dan kontrol lampu grow light sebagai pengganti intensitas cahaya matahari. Penelitian ini telah berhasil membuktikan bahwa pertumbuhan sayuran didalam showcase hidroponik cerdas dapat dipantau dari jarak jauh secara realtime. Pemantauan dilakukan selama ± 18 jam/hari dengan waktu pengambilan data selama 7 hari. Dalam proses pengambilan data terdapat error data 3.25% yang disebabkan koneksi internet terputus pada waktu yang berbeda-beda. Namun ini tidak menyebabkan kegagalan yang signifikan terhadap proses monitoring. Hal ini dibuktikan dengan 8 batang kangkung yang di uji, terdapat 4 batang yang mengalamai gagal tumbuh mulai dari hari ke 4 dan 4 batang lainya tumbuh dengan sempurna.
Strength Values of a Soil Stabilized with Fly Ash, Lime, and Sea Water
Authors: Bilgen Gamze , Doğan Zekeriya
Published: March 2024

In this study, the strength values of a soil to which fly ash (FA) and lime additives treated were examined experimentally. In addition to the additives, the effect of using sea water as kneading water was also examined. In experimental studies, Sieve analysis, Consistency limits, Proctor Test, Unconfined compression (UCS) and California bearing ratio (CBR) tests were carried out on the samples. In addition, X-ray fluorescence (XRF), Fourier transform infrared (FT-IR) and Scanning Electron Microscopy (SEM) analyses were carried out to describe the structural properties of FA. Test results showed that the UCS and CBR values of the soil were 134 kPa and 3.1% respectively. After 28 days of curing, these values increased to 846 kPa and 16.3%, respectively, in the mixture which all additives were used together.
Problematic smartphone use among russian and foreign medical students: prevalence, content consumed, associations with other forms online behavior
Authors: Evert L. S. , Potupchik T. V. , Kostyuchenko Yu. R. , Tereshchenko S. Yu.
Published: March 2024

Aim of the study was to investigate the prevalence of problematic smartphone use, the characteristics of the content consumed and associations with other forms of online behavior among Russian and foreign medical students. Material and methods. 296 medical students were examined using the online survey method: 219 Russians (main group) and 77 foreigners (comparison group); 107 (36.2 %) boys and 189 (63.8 %) girls; age 20 [18; 21] years (median [lower quartile; upper quartile]). The structure of online behavior was assessed: adaptive, non-adaptive, and pathological (PIU) Internet use; specific forms of PIU: problematic use of computer games and social networks, mixed and undifferentiated form of PIU. The values of the total score of the scales of the applied psychometric questionnaires were determined. The indicators were compared in the general group of subjects, in groups of Russians and foreigners, including gender. Results. Problematic smartphone use is typical for 14.2 % of all surveyed. The share of smartphone addicts is higher among foreigners than among Russians (27.3 and 8.0 %, respectively), both boys and girls. PIU prevailed among foreigners (16.1 and 7.4 %), they are more often dependent on social networks (34.5 and 2.4 %). Foreign women were distinguished by a high incidence of problematic use of computer games (20.0 and 3.6 %) and social networks (25.0 and 3.1 %), as well as a mixed form of PIU (20.0 and 2.5 %). The content consumed by Russians (boys + girls) was dominated by being on social networks (77.6 Russians and 46.7 % foreigners) and listening to music (50.7 and 31.2 %, respectively). Gender differences in content included more frequent use of social networks by Russian students, both boys (81.1 and 48.1 %) and girls (76.5 and 43.5 %); more frequent listening to music by Russian males in comparison with foreign males (66.0 and 29.6 %) and Russian females (66.0 and 45.8 %). Foreign boys use smartphones for games more often than foreign girls, the preoccupation with smartphone games is higher among Russian women as well. Conclusions. The prevalence of problematic smartphone use, the characteristics of the content consumed and associations with other forms of PIU among Russian and foreign students were established. In our opinion, it is necessary to carry out further research aimed at solving an urgent medical and social problem of timely diagnosis of smartphonedependent behavior and prevention of somatic and neuropsychic health disorders associated with it in young people. The current range of indicators to be studied in people with problematic smartphone use should include an analysis of comorbid psychosomatic disorders (recurrent pain: headaches, abdominal pain, back pain) and features of psychosocial characteristics, taking into account age, sex and ethnic differences of electronic gadget users.
The Strategic Management In Improving Global Competitiveness At State Madrasah: A Quantitative Swot Analysis
Authors: Marno Marno , Sonia Nur Rahmi , Haris Abdul
Published: March 2024

This research aims to determine the principal's strategy in realizing madrasa competitiveness through an analysis of Strengths, Weaknesses, Opportunities and Threats (SWOT) at Madrasah Aliyah Negeri 2 Ponorogo. This study employed a qualitative approach using a case study research design. Data was collected through in-depth interviews, observation and documentation. Source triangulation and technical triangulation were used to ensure the validity and reliability of the data. Data analysis was carried out by referring to the concepts of Milles, Huberman and Saldana; data collection, data condensation, data presentation and drawing conclusions. The research results show that the head of Madrasah Aliyah Negeri 2 Ponorogo has implemented well strategic management in achieving madrasa competitiveness. This is proven by the achievement of madrasa targets in accordance with the prepared plans. Apart from that, it can be seen from the results of the Strengths, Weaknesses, Opportunities and Threats (SWOT) analysis through detailed internal and external factor analysis in the Internal Factor Analysis Summary (IFAS) and External Factor Analysis Summary (EFAS) matrices. The results of the matrix shows that Madrasah Aliyah Negeri 2 Ponorogo is in quadrant 1. This position shows that the madrasa is in development and growth position. Therefore, Madrasah Aliyah Negeri 2 Ponorogo is advised to use a progressive strategy by utilizing the internal strengths of the madrasah to take advantage of opportunities so that the madrasah's global competitiveness is realized.
Kualitas Pelayanan Akademik terhadap Kepuasaan dan Loyalitas Mahasiswa
Authors: Anwar Wafiq Fadhilah , Malik Adam , Ayuna Nadia , Dewi Anggun Kurnia
Published: March 2024

The relationship between the quality of services provided by higher education institutions and consumer satisfaction is very close. This quality can motivate students to form a strong bond with the university and build loyalty towards it. The purpose of this research is to examine the influence of service quality on student satisfaction and loyalty. The subjects of this study are graduate students in the Islamic Education Management Department at UIN Sunan Gunung Djati Bandung. A quantitative method is employed in this research with a sample size of 76 individuals determined through the Slovin formula and purposive sampling method. The results of the study indicate that service quality significantly affects student satisfaction (Y1). Service quality, as the X variable, also significantly influences student loyalty (Y2). Further understanding of the positive correlation between service quality and student loyalty can assist universities in designing student retention programs and building a committed community on campus. These implications support efforts to enhance the quality of higher education services and establish a sustainable relationship between universities and students.
The Lord of the Flies: Deconstructing the ideas of human civility
Authors: Savadi Raveena
Published: March 2024

‘The Lord of the Flies’ penned by Henry Golding challenges the notions and ideas we as human beings perceive ourselves to be, civilised, good-natured and kind, as compared to who we are in actuality. Golding, inspired by his own experience, crafts this brilliant narrative that wills its viewers to realise the sheer savagery human beings possess behind a veil of civility. It follows the popular narrative of characters having lost their way and finding themselves stranded on a deserted island. However, instead of exciting adventures with promises of mirth and triumph over the brutal forces of nature, Golding unravels his story to reveal the darkness and bloodlust in human nature that instantly begins to project on the loss of social order. He conveys his perceptions of the need for governance over humans because they are inherently violent. This research paper analyses the relationship between the characters to concepts of civility and savagery and explores the story’s subversion of the colonial narrative present in various other novels of the same trope. It also hopes to convey the criticism of the novel and its messaging and explain the significance and timeless nature of the novel that remains relevant to date.
Entrepreneurial Orientation in the Business Profit Development Center of SMEs in Java: The Critical Role of Islamic Values
Authors: Riyoko Sisno , Mufid Fathul
Published: March 2024

Small and medium enterprises (SMEs) are urged to enhance their business operations in the rapidly evolving technological landscape. The emphasis on entrepreneurial orientation has played a crucial role in both economic and societal development. Islamic values, which are rooted in fundamental religious principles, have significant potential to shape entrepreneurial behaviors. This research aims to provide a framework that enhances the performance of SMEs. The results of this research emphasize the significant role of Islamic values in amplifying entrepreneurial orientation. This research reveals that adopting Islamic principles has the potential to significantly improve the business performance of SMEs in Central Java. In addition, SMEs benefit from a significant boost in their performance by embracing Islamic values, such as proactiveness, a commitment to learning, and the willingness to take risks.
Распространение вариантов вируса SARS-COV-2, вызывающих интерес (VOI) и находящихся под наблюдением (VUM), на основе количества их геномов, депонированных в базу данных GISAID за неделю с 2 по 7 марта 2024 г.
Authors: Дмитриева Людмила , Чумачкова Елена , Гусева Наталья , Осина Наталья , Зимирова Анастасия , Иванова Александра , Карнаухов Игорь , Караваева Татьяна , Щербакова Светлана , Кутырев Владимир
Published: March 2024

В обзоре представлена информация по циркулирующим в настоящее время вариантам вируса SARS-COV-2 Omicron вызывающих интерес (VOI) и находящихся под наблюдением (VUM), геномные последовательности которых размещены в международной базе данных GISAID за неделю с 2 по 7 марта 2024 г.
Tom40 Functions as a Channel for Protein Retrotranslocation in the Mitochondria-Associated Degradation (MAD) Pathway
Authors: Pon Liza , Liao Pin-Chao , Tsang Catherine , Lin Tzu-Ying
Published: March 2024

The mitochondria-associated degradation pathway (MAD) mediates removal and elimination of unfolded mitochondrial proteins by the ubiquitin-proteasome system (UPS). Here, we reconstituted retrotranslocation of MAD substrates from the mitochondrial matrix across mitochondrial inner and outer membranes in cell-free systems. This retrotranslocation is ATP- dependent but membrane potential-independent. We also identified a role for the TOM complex, the protein import channel in the mitochondrial outer membrane (MOM), in this process. Inhibition of protein translocation across the Tom40p channel inhibits retrotranslocation of MAD substrates in vitro. Deletion of the TOM5 subunit of the TOM complex inhibits retrotranslocation in vitro, and results in increased sensitivity to oxidative stress and decreased association of a MAD substrate with Cdc48p, a subunit of the segregase complex that removes proteins from mitochondria and targets them for proteasomal degradation in MAD, in vivo. Our studies indicate that unfolded proteins in the mitochondrial matrix are retrotranslocated across both mitochondrial membranes and the TOM complex is the retrotranslocation channel in the MOM in MAD.
Assessing five-year trend and socio-demographic determinants of caesarean section delivery in Ghana
Authors: Manyeh Alfred Kwesi , Ofosu Anthony , Kuug Anthony Kolsabilik , Ayi Atsu , Tetteh Comfort Dede , Vidzro Saviour Edem , Odopey Christopher Tetteh , Ackon Angela Nana Esi Ackon , Acquah Evelyn , Klu Desmond , Immurana Mustapha , Dalaba Maxwell Ayindenaba , Amu Hubert
Published: March 2024

Introduction:The rate of caesarean section (C-section) deliveries has been increasing globally, including in low- and middle-income countries like Ghana. Understanding the trends, patterns, and socio-demographic determinants of C-section deliveries is crucial for improving maternal healthcare services and reducing unnecessary surgical interventions. This study aims to assess the trend and factors associated with CS deliveries in Ghana using secondary data from the District Health Information Management System 2 (DHIMS-2) database.
Methods:A cross-sectional study design was employed, utilizing secondary data extracted from the District Health Information Management System 2 (DHIMS-2) database covering a period of five years (2017-2021). The data included information on C-section deliveries from both public and private health facilities in Ghana. Descriptive and inferential analysis was conducted to explore the associations between socio-demographic factors and C-section delivery.
Results:The study findings revealed that a significant proportion of women who underwent C-section deliveries had formal education up to the JHS/Middle school level. Most of the deliveries occurred in hospitals and government-owned facilities. The study also observed that more than half of the women had a C-section delivery, with the highest percentage occurring in 2019 and 2020. Additionally, the majority of women had health insurance coverage.
Conclusion:The high C-section rate in Ghana may be attributed to various factors such as increasing availability and accessibility of C-section deliveries, socio-demographic factors, and regional disparities in healthcare access and resources. Advanced maternal age, higher levels of education, and formal employment were found to be associated with a higher likelihood of C-section deliveries.
These findings highlight the need for a comprehensive and patient-centered approach to maternal healthcare in Ghana, which includes efforts to strengthen antenatal care services, promote natural childbirth, improve access to quality healthcare facilities, enhance healthcare provider training, address regional disparities, and strengthen health insurance coverage. By addressing these recommendations, Ghana can strive towards reducing unnecessary caesarean section deliveries and improving maternal health outcomes for women across the country.
Oral Microbiome Dynamics in Head and Neck Cancer Patients Undergoing Oral Healthcare: Implications for Prognosis and early diagnosis
Authors: Ji Xiaoqin , Qiao Yixin , Wu Jiqiu , Kong Weili , Yu Rong , Yang Xiuli , Deng Xin , Yu Haopeng , Zhao Huiling
Published: March 2024

Background: This study aims to investigate the influence of two oral mouthwash interventions on the oral microbiome of HNC patients at multiple time points, while simultaneously assessing their prognostic significance. Additionally, it seeks to identify distinctive oral microbiome biomarkers that are linked to different subtypes of HNC.
Methods: Patients with HNC were subjected to random allocation into two distinct oral mouthwash intervention groups: the normal saline group denoted as N and the yikou gargle group employing chlorhexidine, represented as Y. Saliva samples were collected at three time points: baseline, post-operation, and before discharge from hospital. We employed 16S rRNA sequencing to comprehensively profile the composition of the oral microbiome, conducted an in-depth comparison of community diversity, and pinpointed enriched taxa within each oral mouth wash intervention group. Simultaneously, we conducted an investigation into the potential correlation between specific oral microbes and oral prognostic outcomes.
Results: This study included 80 patients diagnosed with HNC scheduled for surgical intervention, and 30 patients underwent tracheostomy. Initially, both treatment groups had similar oral microbiome diversity. However, after surgery, the Y group exhibited a higher Shannon index and greater pronounced clustering variation compared to the N group. The genus Streptococcus dominated the oral microbiomes of both groups. The Y group showed a heightened abundance of taxa immediately after surgery when compared to the N group. In terms of implications for oral healthcare on oral prognosis, a notable finding was the significantly reduced severity of dental issues among patients in Y group when underwent tracheostomy as opposed to those in N group (p = 0.019).
Conclusion: Our study provides insights into the impact of oral mouthwash interventions on the oral microbiome of HNC patients and their potential implications for prognosis. Understanding the role of the oral microbiome in HNC may pave the way for innovative therapeutic strategies that target the oral microbiota to improve treatment outcomes. Disparities in the oral microbiome among various types of HNC also suggest the potential for straightforward and non-invasive diagnostic approaches utilizing the oral microbiome.
Climate change, circular economy and foodborne pathogens: assessment of pathogenic E. coli and Salmonella spp. in irrigation water in Italy
Authors: Barbieri Giorgia , Gigliucci Federica , Palma Sofia , Minelli Fabio , Chiani Paola , Marra Manuela , Carollo Maria , Knijn Arnold , Brambilla Gianfranco , Morabito Stefano
Published: March 2024

The use of reclaimed water from wastewater treatment plants (WWTPs) in agriculture represents a sustainable way to reduce water footprint. However, outbreaks linked to the consumption of contaminated ready-to-eat food (RTE) have frequently been associated with the presence of foodborne pathogens in irrigation water. To deepen the hazard characterization of water used for crop irrigation, reclaimed and surface water samples (n=15), collected in Northern Italy in spring and summer, were analysed via Real Time PCR, before and after an enrichment treatment, to detect virulence genes of STEC (Shiga toxin producing - E. coli); EPEC (Enteropathogenic E. coli); EAEC (Enteroaggregative E. coli); EIEC (Enteroinvasive E. coli); ETEC (Enterotoxigenic E. coli) and Salmonella spp. Virulence genes of Salmonella spp. and all E. coli pathogroups were detected in 14/15 samples. 11/14 samples showed the presence of pathogenic E. coli both pre- and post-enrichment. The most detected genes were stx1, stx2, eae, followed by aggR, aaiC, ipaH, lt, stp, sth genes. Four enriched samples were positive for invA gene. Four strains were isolated from enriched samples, including one STEC (stx1+) strain and two Salmonella Veneziana strains from surface water samples, and one strain of S. Taksony from a WWTP sample. The detection of viable pathogens in irrigation water suggests that the depurative treatments may not be fully effective in eliminating pathogenic E. coli and Salmonella spp. in reclaimed water. A conditioned use of surface water is needed to reduce the pathogen input on crops. Furthermore, the higher positive signals recorded after rainfalls suggest that pathogen mobilization is linked to precipitation patterns. Since the detection of pathogenic E. coli in agricultural water is not regulated by the Italian and European legislation, except for STEC O157 in spent irrigation water, their likely presence should be adequately considered and risk-oriented, depending on climatic conditions, processing technologies and type of agricultural use.
Secular variation and risk factors of preterm birth in Hainan, The Free Trade Port in China from 2010 to 2021
Authors: Luo Qing , Li Chao , Wu Weijia , Zhao Anping , Zhao Dan , Huang Chuican , Fan Lichun
Published: March 2024

Objectives
Preterm delivery rates have increased obviously worldwide over the past decade, yet reliable epidemiological studies on the incidence of preterm birth and temporal trends are not available in Hainan, The Free Trade Port in China. We aimed to describe the rate of preterm birth and trends between 2010 and 2021 and to primarily explore risk factors associated with preterm birth in Hainan, China.
Methods
This was an observational study was based on data from the Hainan Provincial Birth Certificate System (HPBCS) for live births between 1 January 2010 and 31 December 2021.Pregnancies with at least one live birth, with the baby born at 28 weeks of gestation or more or 1000 g or more birthweight were included.The outcome were preterm birth rates and their trends over time. Potential risk factors were collected, including infant’s date of birth, infant gender, maternal age, paternal age, maternal ethnicity, paternal ethnicity, gestational age at delivery, mode of delivery, and single or multiple pregnancies. The logistic regression model was used to assess the relationship between preterm birth and potential risk factors.
Results
A total of 1,537,239 live births and 86,328 preterm births were investigated, giving a total preterm birth rate of 5.6%. The overall preterm birth rate increased from 5.62% in 2010 to 7.1% in 2021 (compound annual growth rate [CAGR] 4.3). The CAGR of late preterm birth is consistent with the overall preterm birth rate (4.3%). The fastest growth is observed in the rate of early preterm births (5.5%), while the rate of mid-term preterm births exhibits the slowest growth (3.9%). Infant gender, multiple pregnancy, parental age, and ethnicity had significant effects on preterm birth.
Conclusion
The preterm birth rate was increasing year by year from 2010 to 2021 in Hainan, The Free Trade Port in China. Multiple pregnancies, higher maternal age at delivery, and male infants were associated with a higher rate of preterm birth.
Sedimentary characteristics and evolution of the Upper Permian Wujiaping Formation in the eastern Yangtze Block
Authors: Wang Yuanchong , Li Hao , Mou Chuanlong , Chen Weiwei , Gao Chang , Liang Wei , Zheng Binsong , Hou Qian , Xia Yu
Published: March 2024

The Upper Permian Wujiaping (WJP) Formation in the Yangtze Block (Southern China) has been proven of great potential for shale gas exploration. However, the sedimentary characteristics and environment evolution of WJP Formation is poorly understood. Based on 260 hand specimen samples obtained from 18 sections and 1 borehole in eastern Yangtze Block, petrographic observation reveals that the WJP Formation is composed of ten kinds of rock types. Combining with analyses of geochemistry compositions, the sedimentary facies belts of the WJP Formation are divided into shoreland, shallow-water shelf, deep-water shelf, and shoal. Bauxite was developed in the shoreland at the bottom of the WJP Formation, which is attributed to the Dongwu Movement. The formation of paleo-uplift exerted significant effect on palaeogeomorphology and led to major change of the sedimentary model from underlying stratum to WJP Formation. During the lower WJP Formation sedimentary period, gradual transgression occurred in the eastern Yangtze Block. Shoreland, shallow-water shelf, and deep-water shelf were developed northward in turn. In the period of the upper WJP, the area of the deep-water shelf was expanded due to durative transgression. A few patched shoals formed on the shallow-water shelf.
Dynamics analysis and parameter optimization of a nonlinear energy sink with geometrically nonlinear inerters
Authors: Jiawen Chu , Jingjun Lou , Chai kai
Published: March 2024

This paper studies a nonlinear energy sink (NES) with geometrically nonlinear inerters (GNI-NES) to improve the vibration absorption performance. The force-displacement relation of the geometrically nonlinear inerters mechanism (NIM) is obtained, and a dynamic model is established for the coupled system. The dynamic responses of the system are solved using the harmonic balance method (HBM) and the Runge-Kutta method. The vibration absorption performance of the system is evaluated using displacement transmissibility, and the parameters are analyzed. Then, the grey wolf algorithm is used to obtain the optimal system parameters. The results show that compared with the cubic NES, the proposed GNI-NES achieves the lower peak value, and exhibits better vibration stability compare to the cubic NES. Furthermore, these outcomes enhance our comprehension of the operations of the NIM and aid in crafting more effective designs of nonlinear passive vibration mitigation systems employing inerters.
Dumbbell probe-based colorimetric and sensitive detection of miRNA during postoperative care of gastric cancer by using polymerase/endonuclease assisted chain displacement
Authors: Xu Xialing , Zhang Ping , Tao Siyu
Published: March 2024

Methods for sequence-specific microRNA (miRNA) analysis that are both simple and quick to implement are crucial for miRNA research and guiding nursing strategies. We have devised a colorimetric technique for detecting miRNA using a d dumbbell probe-based polymerase/endonuclease assisted chain displacement, along with silver ions (Ag+) aptamer assisted color reaction. This method utilizes the hairpin structure in the dumbbell probe to significantly enhance the accuracy of target recognition in the polymerase/endonuclease assisted chain displacement strategy. As a result, the method gains a superior capability to discriminate between target miRNA and interfering miRNAs. The combination of target recycling and the polymerase/endonuclease assisted chain displacement strategy allows for highly sensitive analysis of miRNA, which is either superior or comparable to previous colorimetric methods for miRNA detection. Hence, the suggested approach enables precise measurement of miRNA-21 within the concentration range of 100 fM to 5 nM, with a low detection limit of 45.32 fM. Additionally, it exhibits exceptional capability in distinguishing variations at the level of individual nucleotides. Furthermore, the detection technique may be utilized to precisely measure the amount of miRNA-21 in serum samples, demonstrating a high level of concordance with the findings obtained from a commercially available miRNA detection kit. This approach is simple, economical, and extremely specific for detecting miRNA. It holds great potential for clinical use, particularly in guiding the adaptation of nursing techniques.
Safety and surgical outcomes of robotic adrenalectomy: a 15-year experience at a single institution
Authors: Kim Kwangsoon , Park Dawon , Oh Moon Young , Chai Young Jun , Kim Hoon Yub
Published: March 2024

Robotic adrenalectomy (RA) has gained significant popularity in the management of adrenal gland diseases. We report our experience at a single tertiary institution and evaluate the safety and surgical outcomes of RA. The data of 122 consecutive patients who underwent RA from October 2009 to December 2022 at Korea University Anam Hospital (Seoul, Korea) were reviewed. Clinicopathological features and surgical outcomes were retrospectively analyzed through complete chart reviews. Noteworthy findings include the influence of sex, tumor size, and body mass index on operation time, with the female and small tumor groups exhibiting shorter operation times (P = 0.018 and P = 0.009, respectively). Pheochromocytoma was identified as a significant independent risk factor for a longer operation time in the multivariate analysis (odds ratio[OR], 3.709; 95% confidence interval [CI], 1.127–12.205; P = 0.031). A temporal analysis revealed a decreasing trend in mean operation times across consecutive groups, reflecting a learning curve associated with RA adoption. RA is a safe and effective operative technique alternative to laparoscopic adrenalectomy that has favorable surgical outcomes and enhances the convenience of the operation.
Supplementation of Acacia dealbata versus Acacia mearnsii leaf-meal has potential to maintain growth performance of lambs grazing low-quality communal rangelands in South Africa
Authors: Mushunje Lovelyn Hellen , Marandure Tawanda , Chikwanha Obert Chenjerai , Bennett James , Hawkins Heidi , Palmer Antony , Wu Lianhai , Marufu Munyaradzi , Mapiye Cletos
Published: March 2024

Supplementing livestock grazing communal rangelands with leaf-meals from Acacia trees, which are currently considered as problematic invasive alien plants globally, may be a sustainable way of exploiting their desirable nutritional and anthelmintic properties. The current study evaluated worm burdens and growth performance of lambs grazing low-quality communal rangelands supplemented with leaf-meals prepared from the invasive alien plant species; Acacia mearnsii or A. dealbata. Forty, three-month-old ewe lambs weighing an average of 18.9 ± 0.60 kg were randomly allocated to four supplementary diets: (1) rangeland hay only (control), (2) commercial protein supplement plus rangeland hay, (3) A. mearnsii leaf-meal plus rangeland hay and (4) A. dealbata leaf-meal plus rangeland hay. All the supplementary diets were formulated to meet the lambs’ minimum maintenance requirements for protein. All the lambs were grazed on communal rangelands daily from 0800 to 1400 after which they were penned to allow them access to their respective supplementary diets until 08:00 the following morning. The respective supplementary diets were offered at the rate of 400 g ewe− 1 day− 1 for 60 days. Lambs fed the commercial protein supplement had the highest dry matter intake followed by those fed the Acacia leaf-meals and the control diet, respectively (P ≤ 0.05). Relative to the other supplementary diets, lambs fed the commercial protein supplement and A. dealbata leaf-meal had higher (P ≤ 0.05) final body weight and average daily gains. Dietary supplementation did not affect lamb faecal worm egg counts over the study period (P > 0.05). There was no association between supplementary diets and lamb FAMACHA© scores (P > 0.05). It was concluded that the Acacia dealbata relative to Acacia mearnsii has potential to maintain growth performance of lambs grazing communal rangelands in the dry season.
Structural Equation Modeling Analysis of Factors Influencing decisional conflict between dialysis modality among end-stage kidney disease patients in Wuhan
Authors: Zhang shiyi , Cui jinrui , Liu xiaoqin , He xifei , Xu yulin
Published: March 2024

Objectives: To explore the influencing factors and relationships associated with decisional conflict of dialysis modality in ESKD patients.
Methods: This study was a survey-based cross-sectional investigation conducted on 150 ESKD patients in a third-class hospital in Wuhan. The general information questionnaire, decisional conflict scale, Montreal cognitive assessment, frail scale, perceived social support scale, and brief health literacy screen were used for investigation. SPSS 25.0 was used to compare the differences between the decisional and non-decisional conflict groups, and AMOS 23.0 was used to construct a structural equation model to explore the influencing factors.
Results:The incidence of decisional conflict in 150 ESKD patients was 33.3% (50/150). Binary logistic regression analysis showed that the independent risk factors for decisional conflict of dialysis modality in ESKD patients included monthly household income (OR = 0.184), cognitive function (OR = 7.0), social support (OR = 0.891), health literacy (OR = 0.608), the level of eGFR (OR = 1.488), and the level of cTnI (OR = 9.558). The constructed path analysis model had a good fit (x2/df = 1.499, GFI = 0.957, AGFI = 0.911, NFI = 0.906, CFI = 0.967, RMSEA = 0.055). The path analysis showed that health literacy (0.577) had the greatest impact on the decisional conflict, with a direct effect of 0.480 and an indirect effect of 0.097 through cognitive function and monthly household income. Next was social support with an effect value of 0.434.
Conclusions:In clinical practice, it is important to enhance the health literacy of patients and their families and to provide advance education on dialysis plans. Additionally, in managing and planning chronic kidney disease progression and dialysis, it is recommended to regularly and systematically assess cognitive function, particularly before the patient's cognitive impairment worsens or the severity of the disease progresses. Advance care planning can be established through collaboration between healthcare professionals and patients to ensure appropriate decision-making and management.
Implications for the profession and/or patient care：This paper finds that the influencing factors and their relationships of dialysis methods in end-stage renal disease patients,helping nurses better exercise autonomy, helping patients reduce their decisional conflict , improving clinical outcomes.
Patient or Public Contribution： Patients received a relevant questionnaire survey, and caregivers assisted in conducting the survey.
Can the Pan-immune-inflammation Value Predict Gram Negative Bloodstream Infections-related 30-day Mortality in Solid Organ Transplant Recipients?
Authors: Ayaz Caglayan Merve , Turhan Özge , Yılmaz Vural Taner , Adanır Haydar , Sezer Beyza , Öğünç Dilara
Published: March 2024

Background: The recently used pan-immune-inflammation value (PIV) has not been adequately studied as a predictive marker for mortality in immunosuppressed patients. The aim of this study was to evaluate the usefulness of baseline PIV level as a predictor of 30-day mortality in solid organ transplant (SOT) recipients with gram negative bloodstream infections (GN-BSI).
Methods: This retrospective, cross-sectional study was conducted between January 1, 2019, and December 31, 2022, in 1104 SOT recipients. During the study period, 118 GN-BSI were recorded in 113 patients. Clinical, epidemiological, and laboratory data were collected, and mortality rates (30-day and all-cause) were recorded.
Results: The 113 recipients had a median age of 50 years [interquartile range (IQR) 37.5-61.5 years] with a male predominance (n=72, 63.7%). The three most common microorganisms were as follows: 46 isolates (38.9%) of Escherichia coli, 41 (34.7%) of Klebsiella pneumoniae, and 12 (10.2%) of Acinetobacter baumannii. In 44.9% and 35.6% of the isolates, production of extended-spectrum beta-lactamases and carbapenem resistance were detected, respectively. The incidence of carbapenem-resistant GN-BSI was higher in liver recipients than in renal recipients (n=27, 69.2% vs n=13, 17.6%, p<0.001). All-cause and 30-day mortality rates after GN-BSI were 26.5% (n=30), and 16.8% (n=19), respectively. In the group with GN-BSI-related 30-day mortality, the median PIV level was significantly lower (327.3, IQR 64.8-795.4 vs. 1049.6, IQR 338.6-2177.1; p= 0.002). The binary logistic regression analysis identified low PIV level [hazard ratio (HR)=0.93, 95% confidence interval (CI) 0.86-0.99; p= 0.04], and increased age (HR=1.05, 95% CI 1.01-1.09; p= 0.002) as factors associated with 30-day mortality. The receiver operating characteristic analysis revealed that PIV could determine the GN-BSI-related 30-day mortality with area under curve (AUC): 0.723, 95% CI 0.597-0.848, p = 0.0005.
Conclusions: PIV is a simple and inexpensive biomarker that can be used to estimate mortality in immunosuppressed patients, but the results need to be interpreted carefully.
Temperature-based protection of biochemical and biomechanical characteristics during 25 kGy 60Co irradiation for allograft tendons
Authors: Wan Yanlin , Liu Dayong , Wang Xiaolong , Wang Limin , Ma Rongxing , Jiang Zhao , Li Wenjing , Gao Bing , Zhang Jingyu , Hu Yongcheng
Published: March 2024

Background: Nowadays, there is a lack of effective treatment for reducing damage to the collagen proteins in tendons during the gamma irradiation process. We aim to investigate different temperatures on the protection of allogeneic tendons during 25kGy 60Co gamma irradiation.
Methods: Based on different temperatures during irradiation, twenty-four allogeneic tendons were divided into groups of -70 °C, 0 °C, and room temperature (RT). The arrangement and spatial structure of collagen fibers were observed by HE and Masson staining, polarizing microscope after Sirius Red staining was used to observe collagen curl periods, damaged collagen protein was assessed using free hydroxyproline tests. Tensile fracture and cyclic loading tests were performed, then calculated the elastic modulus, maximum stress, maximum strain, strain energy density, and cyclic creep strain.
Results: In the -70 °C group, the structure and morphology of collagen fibers were significantly better than in the 0 °C group. At -70 ºC the damaged collagen was the lowest, with 46.27 and 50.03% improvement compared to that at 0ºC (P = 0.016) and RT (P < 0.004). The maximum stress of -70 °C (68.19 ± 30.11 MPa) is superior to that at 0 °C (36.58 ± 12.00 MPa) and RT (41.27 ± 16.34 MPa).
Conclusions: Cryogenic temperature has a protective effect on allograft tendons at 25kGy gamma radiation, and -70 °C provides better protection than 0 °C
A small-molecule SARS-CoV-2 inhibitor targeting the membrane protein
Authors: Loock Marnix Van , Damme Ellen Van , Abeywickrema Pravien , Yin Yanting , Xie Jiexiong , Jacobs Sofie , Mann Mandeep , Miller Robyn , Piassek Madison , Doijen Jordi , Marsili Simone , Subramanian Murali , Gottlieb Leah , Abdelnabi Rana , Gool Michiel Van , Broeck Nick Van den , De Pauw Ines , Scobey D. , Mattocks Melissa , Schaefer Alexandra , Jochmans Dirk , De Jonghe Steven , Leyssen Pieter , Chiu Winston , Toro Mayra Diosa , Zwaagstra Marleen , Leijs Anouk , de Gruyter Heidi , Buyck Christophe , Heede Klaas van den , Jacobs Frank , Eynde Christel Van den , Thijs Laura , Raeymaekers Valerie , Miller Seth , Rosario Amanda , Neyts Johan , Baric Ralph , Kuppeveld Frank van , Snijder Eric , Hemert Martijn van , Monshouwer Mario , Sharma Sujata , Draghia-Akli Ruxandra , Koul Anil , Diels Annick , Vermeulen Peter , Temmerman Koen , Peeters Danielle
Published: March 2024

The membrane (M) protein of betacoronaviruses is well-conserved and plays a key role in viral assembly. Here, we describe the discovery of JNJ-9676, a novel small molecule targeting the coronaviral (CoV) M protein. JNJ-9676 demonstrates in vitro nanomolar antiviral activity against SARS-CoV2, SARS-CoV, and sarbecovirus strains from bat and pangolin zoonotic origin. Using cryogenic electron microscopy, we determined a novel binding pocket of JNJ-9676 in the M protein's transmembrane domain. Compound binding stabilized the M protein in an altered conformational state between its long- and short-forms, preventing the release of infectious virus. In a pre-exposure Syrian golden hamster model, JNJ-9676 (25 mg/kg BID) showed excellent efficacy illustrated by a significant reduction in viral load and infectious virus in the lung by 3.5 log10 and 4 log10, respectively. Histopathology scores at this dose were reduced to baseline. In a post-exposure hamster model, JNJ-9676 was efficacious at 75mg/kg BID even when added at 48 h post-infection, when peak viral loads were observed. The M protein is an attractive novel antiviral target to block coronavirus replication with JNJ-9676 representing an interesting chemical series towards identifying clinical candidates addressing the current and future CoV pandemics.
The variations of five Colletotrichum strains from strawberry in mycelial growth and pathogenicity below 26℃
Authors: Li Xue , Geng Chunnu , Huang Xianyao , Chen Siyu , Yang Jing , Han Yongchao , Lu Fangyan , Duan Ke , Gao Qinghua
Published: March 2024

Anthracnose caused by Colletotrichum spp. is a serious threat to many crops. C. siamense has been increasingly prevalent recently. However, the biology and pathogenicity of these fungi was largely elusive, especially below 26°C. Currently, the mycelial growth of five representative Colletotrichum strains including three C. siamense, one C. fructicola and one C. gloeosporioides s.s. strain from diseased strawberry was characterized at 10, 14, 18, 22 and 26℃. Their pathogenicity to two hosts under 14℃ and 22℃ was investigated. These strains did not differ much in mycelium growth between 10–26℃, although all C. siamense and C. gloeosporioides s.s. strains showed some superiority than C. fructicola. However, great variations were observed in their pathogenicity to Fragaria vesca and F. × ananassa cv. ‘Benihoppe’. The strain Cs:GQHZJ19 with fastest mycelial growth at 22℃ was also the most virulent to both hosts under 14 / 22℃. But the mycelial growth rate was only positively correlated with C. siamense pathogenicity to the susceptible cv. ‘Benihoppe’ at 3 dpi under 22℃. Based on the pathogenicity analysis for five Colletotrichum strains on hosts with varying susceptibility under distinct temperatures, this work suggested that host genotype might play a decisive role in disease severity from the beginning of infection, while temperature could shape host-Colletotrichum spp. interaction at a later infection stage. Future studies with more fungal samples are required to determine the biology and virulence of Colletotrichum strains (especially C. siamense) in a wider temperature range, which will eventually facilitate an effective management of crop anthracnose.
Effect of serum uric acid on prognosis of drug-eluting stent implantation for patients with coronary bifurcated lesions
Authors: Gao Hongjie , Liu Dongchao , Xue Zheng , Qi Jingxian , Yin Liang , Duan Bing , Wu Lin , Yang Kun , Gao Bulang , Cao Qinying , Mi Jie
Published: March 2024

Purpose
To investigate the effect of hyperuricemia on prognosis of drug-eluting stent implantation for patients with coronary bifurcated lesions.
Materials and methods
Patients with coronary bifurcation lesions treated with drug-eluting stent implantation were retrospectively enrolled. The clinical, interventional and follow-up data were analyzed.
Results
Totally, 193 patients were enrolled, including 59 with hyperuricemia and 134 without hyperuricemia. Serum triglyceride (1.73 vs. 1.44 mmol/L) and creatinine (70.0 vs. 61.0µmol/L) levels were significantly (P < 0.05) higher in the hyperuricemia than in the non-hyperuricemia group. During follow-up, seven patients died, including one (14.3%) death in the hyperuricemia and six (85.7%) in the non-hyperuricemia group. Twelve patients experienced revascularization, including 5 (41.7%) in the hyperuricemia group and 7 (58.3%) in the non-hyperuricemia group. Seventy patients were readmitted to the hospital because of cardiac reasons, including 36 (51.4%) patients in the hyperuricemia and 34 (48.6%) in the non-hyperuricemia group. The risk of cardiac readmission was significantly (P < 0.001) greater in the non-hyperuricemia than in the hyperuricemia group (30.10 vs. 24.33 m). Age was a significant (P = 0.01) independent risk factor for all-cause mortality after cardiac intervention with a hazard ratio (HR) of 1.12 (95% CI 1.03–1.23). Diabetes mellitus was the only significant (P = 0.007) independent risk factor for revascularization, with a HR of 5.13 (95% CI 1.56–16.91). Male sex (P = 0.023, HR 2.21 with 95% CI of 1.12–4.38), total cholesterol (P = 0.004, HR 2.53 with 95% CI 1.35–4.74), and hyperuricemia (P = 0.001, HR 2.37 with 95% CI 1.44–3.89) were significant independent risk factors for cardiac readmission.
Conclusion
Hyperuricemia is an independent risk factor for cardiogenic readmission rather than for all-cause mortality and revascularization after drug-eluting stent implantation for patients with coronary bifurcation lesions.
Numerical simulation of potential impulse waves generated by Mogu rock landslide at varying water levels in Lianghekou Reservoir, China
Authors: Chen Shizhuang , Xu Weiya , Zhang Guike , Wang Rubin , Yan Long , Zhang Hailong , Wang Huanling
Published: March 2024

Reservoir impoundment and water level fluctuations often trigger landslides and their secondary disasters, such as potential impulse waves, posing a serious threat to the safety of people along the reservoir and dam areas, causing economic losses and even catastrophic consequences. This study delves into a comprehensive field investigation and monitoring of the engineering geological conditions and deformation mechanisms the Mogu rock landslide. Impoundment is identified as the primary factor inducing slope deformation, with the cumulative displacement of the sliding body showing no signs of convergence, indicating potential instability. By coupling the elasto-visco-plasticity model and the RNG turbulence model in FLOW3D, an actual surge disaster near the Lianghekou Reservoir dam area is replicated to validate the reliability of the numerical method. Building upon this, a three-dimensional model is established to calculate potential impulse waves generated by the Mogu rock landslide, and the risk to the dam is evaluated. Under varying water level conditions, the simulated heights of impulse waves do not surpass the dam elevation, demonstrating a satisfactory safety margin. Given the inherent danger of landslide-induced wave disasters, continuous attention is warranted, and preventive measures and suggestions are proposed to address these concerns. Additionally, the study explores the contributions of water level fluctuations to the primary wave height, the maximum run-up on the opposite bank and the dam, and the attenuation rate of wave height along the river channel. The results provide significant reference values for the early warning and prevention of comparable reservoir landslides and potential landslide-induced waves worldwide.