bluuebunny/crossref_metadata_2025 huggingface dataset:
Prophage enhances the ability of deep-sea bacterium
Shewanella psychrophila
WP2 to utilize D-amino acid
Authors: Tan Xiaoli , Zhang Mujie , Liu Shunzhang , Xiao Xiang , Zhang Yu , Jian Huahua
Published: January 2024

Prophages are prevalent in the marine bacterial genomes and reshape the physiology and metabolism of their hosts. However, whether and how prophages influence the microbial degradation of D-amino acids (D-AAs), which is one of the widely distributed recalcitrant dissolved organic matters (RDOMs) in the ocean, remain to be explored. In this study, we addressed this issue in a representative marine bacterium,
Shewanella psychrophila
WP2 (WP2), and its integrated prophage SP1. Notably, compared to the WP2 wild-type strain, the SP1 deletion mutant of WP2 (WP2ΔSP1) exhibited a significantly lower D-glutamate (D-Glu) consumption rate and longer lag phase when D-Glu was used as the sole nitrogen source. The subsequent transcriptome analysis identified 1,523 differentially expressed genes involved in diverse cellular processes, especially that multiple genes related to inorganic nitrogen metabolism were highly upregulated. In addition, the dynamic profiles of ammonium, nitrate, and nitrite were distinct between the culture media of WP2 and WP2ΔSP1. Finally, we provide evidence that SP1 conferred a competitive advantage to WP2 when D-Glu was used as the sole nitrogen source and SP1-like phages may be widely distributed in the global ocean. Taken together, these findings offer novel insight into the influences of prophages on host metabolism and RDOM cycling in marine environments.

IMPORTANCE

This work represents the first exploration of the impact of prophages on the D-amino acid (D-AA) metabolism of deep-sea bacteria. By using
S. psychrophila
WP2 and its integrated prophage SP1 as a representative system, we found that SP1 can significantly increase the catabolism rate of WP2 to D-glutamate and produce higher concentrations of ammonium, resulting in faster growth and competitive advantages. Our findings not only deepen our understanding of the interaction between deep-sea prophages and hosts but also provide new insights into the ecological role of prophages in refractory dissolved organic matter and the nitrogen cycle in deep oceans.
Luteolin attenuates
Staphylococcus aureus
-induced endometritis through inhibiting ferroptosis and inflammation via activating the Nrf2/GPX4 signaling pathway
Authors: Gao Shouyang , Gao Yongjian , Cai Lifu , Qin Rui
Published: January 2024

Endometritis, a local inflammatory disease, has been known as the most common cause of infertility in mares. In this study, we investigated the protective effects of luteolin on endometritis induced by
Staphylococcus aureus
(
S. aureus
) and further clarified the possible molecular mechanisms. An
S. aureus
–induced endometritis model was established by the infusion of
S. aureus
into the uterus. Luteolin was intraperitoneally administered to mice 1 h before
S. aureus
treatment. The results showed that the mice of the
S. aureus
group showed severe histological changes of uterine tissues, increased myeloperoxidase (MPO) activity, and elevated TNF-α, IL-1β, and IL-6 levels. These changes induced by
S. aureus
were dose-dependently inhibited by luteolin. Furthermore, luteolin inhibited MDA and Fe
2+
production and increased the production of GSH decreased by
S. aureus
. Luteolin prevented
S. aureus
–induced endometrial barrier disruption through up-regulating ZO-1 and occludin expression. Luteolin dramatically inhibited
S. aureus
–induced NF-κB activation. The expression of Nrf2 and HO-1 was increased by luteolin. In addition, the inhibitory effects of luteolin on
S. aureus
–induced endometritis were reversed in Nrf2 knockdown mice. In conclusion, these data indicated that luteolin protected mice against
S. aureus
–induced endometritis through inhibiting inflammation and ferroptosis via regulating the Nrf2 signaling pathway.

IMPORTANCE

Endometritis is an inflammatory disease of the endometrium, which is a common gynecological disease. Up to now, there is no evidence for the protective effects of luteolin on endometritis. The purpose of this study was to investigate whether luteolin has protective effects against
S. aureus
–induced endometritis and attempts to clarify the mechanism.
A salty snapshot: extreme variations in basal erosion patterns preserved in a submarine channel
Authors: Casagrande Junia , Hodgson David M. , Peakall Jeff
Published: January 2024

The bases of active submarine channels are marked by large erosional features, such as knickpoints and plunge pools. However, their presence in ancient channel-fills has rarely been documented, so their importance in submarine channel morphodynamics requires investigation. Using seismic reflection data calibrated by wells from a buried submarine channel-fill, we document erosional features hundreds of metres long and tens of metres deep, here interpreted as knickpoints and a plunge pool, and provide a mechanistic process for their transfer into the stratigraphic record. Channel incision patterns are interpreted to record a transient uplift in an otherwise subsiding depocentre. Local structural complexities in the channel slope formed zones of preferential scouring. A switch to a depositional regime preserved the irregular channel base, inhibiting both the upstream migration of scours and smoothing of the channel base. The formation and preservation of these scours record the responses to salt tectonics and provide a unique snapshot of the formative processes of an ancient submarine channel. The presence of these exceptional basal scours indicates that headward erosion processes did not operate rapidly, challenging the paradigm that knickpoint migration controls channel evolution. Our results show that the primary erosion of the main channel surface and long-term channel evolution are both dominated by far more gradual processes.
Comparative Analysis of Loss Mechanism Localization in a Semi-2D SOEC Single Cell Modell: Non-Equilibrium Thermodynamics versus Monocausal-Based Approach
Authors: Gedik Aydan , Wachtel Jonas , Kabelac Stephan
Published: January 2024

This study aims to theoretically analyze the local entropy production rate in a SOEC single cell at T = 1123.15 K and p = 1 bar. Local entropy rates signify loss mechanisms, crucial for cell design and optimization. A semi-2D SOEC model based on non-equilibrium thermodynamics is developed, supplemented by monocausal correlations for direct comparison. The model is validated using KeraCell III data and grid independence analysis. Simulations of electric current density, temperature, heat flow, and local entropy production for various SOEC operating modes are presented. Coupled transport mechanisms significance is discussed, highlighting the pronounced impact of the Peltier effect on heat flux and temperature. The importance of the Peltier effect in SOECs compared to SOFCs is emphasised. The effects of the Seebeck effect on the potential distribution are superimposed by the dominant ohmic losses in the electrolyte. The localization of entropy production rates shows for exothermic operation that 66.6% of the total losses are due to the predominantly dominant irreversible ion transport in the electrolyte, while the entanglements in the reaction layers contribute 33% and GDLs less than 1%.
Mechanosensory encoding of forces in walking uphill and downhill: force feedback can stabilize leg movements in stick insects
Authors: Zill Sasha N. , Dallmann Chris J. , Zyhowski William , Chaudhry Hibba , Gebehart Corinna , Szczecinski Nicholas S.
Published: January 2024

Discharges of sensory receptors (campaniform sensilla) in the hind legs of stick insects can differentially signal forces that occur in walking uphill versus walking downhill. Unexpectedly, sensory firing most closely reflects the rate of change of force (dF/d t) even when the force levels are high. These signals have been replicated in a mathematical model of the receptors and could be used to stabilize leg movements both in the animal and in a walking robot.
Omeprazole Induced Rapid Drug Reaction with Eosinophilia, Systemic Symptoms, and Cross-Reactivity in Delayed-Type Hypersensitivity Associated with Proton-Pump Inhibitors: A Case Report and Literature Review
Authors: Pinyopornpanish Kanokkarn , Pinyopornpanish Kanokporn , Pinyopornpanish Kanokwan , Benjanuwattra Juthipong , Teepapan Putthapon , Chungcharoenpanich Apinya , Laisuan Wannada
Published: January 2024

Background. Omeprazole, a proton pump inhibitor (PPI), is a widely used and generally safe agent for treating acid-related gastrointestinal conditions. However, drug reaction with eosinophilia and systemic symptoms (DRESSs) syndrome has been reported. Objectives. To report a case of omeprazole-induced rapid DRESS syndrome and to review the literature. Methods. Descriptive analysis of one new case and a case series from literature review. Results. We report a case of 82-year-old woman presenting with rapid-onset of DRESS syndrome. The condition was initially suspected to be caused by antibiotic, but the definite diagnosis was eventually omeprazole-induced DRESS syndrome as suggested by the enzyme-linked immune absorbent spot (ELISpot) assay along with the clinical picture. Previous literatures regarding cases of PPI-induced DRESS syndrome were pooled for descriptive analysis. Among 21 PPI cases pooled, esomeprazole was the most commonly implicated PPI (52.4%), followed by pantoprazole (19.1%), and omeprazole along with lansoprazole (both 14.3%). The issue of cross-reactivities amongst PPIs remains uncertain. Nonetheless, in situations in which a PPIs are deemed necessary, a prudent approach could be considering a switch to an alternative agent with distinct chemical structure. Conclusion. PPI is commonly used safely as an agent for acid-related gastrointestinal conditions. However, PPI-induced rapid DRESS syndrome can occur, particularly with prior exposure history. ELISpot is an in vitro test, useful in identifying the culprit agent in patients with delayed-type hypersensitivity reaction.
Renal Extramedullary Hematopoiesis in Mast Cell Leukemia with Bone Marrow Fibrosis
Authors: Rieke Damian T. , Schmalbrock Laura K. , Ihlow Jana , Kleo Karsten , von Brünneck Ann-Christin , Nolte Florian , Keller Ulrich , Ochsenreither Sebastian
Published: January 2024

Systemic mastocytosis is defined by the clonal proliferation of abnormal mast cells. The clinical course can range from indolent forms with normal life expectancy to advanced mast cell leukemia with dismal prognosis. An association with other diseases, including myeloproliferative neoplasia, has been described. We present a case of a 75-year patient with a history of cutaneous mastocytosis who was diagnosed with mast cell leukemia more than 9 years ago and did not receive treatment. The patient presented to our clinic with acute kidney failure because of renal extramedullary hematopoiesis. Bone marrow histopathology revealed extensive fibrosis and 50% infiltration by mast cells with a c-KIT D816V mutation. No mutations supporting primary myelofibrosis were identified. Treatment with midostaurin was started, and the patient was discharged after improvement of renal function. Here, we discuss diagnostic challenges between different forms of mast cell leukemia and overlaps with other hematological malignancies.
Prevalence Estimation and Genetic Characterization of Porcine Parainfluenza Virus 1 (PPIV‐1) in Hungary and the First Report of the Virus in Slovakia
Authors: Igriczi Barbara , Dénes Lilla , Czétényi Anna , Révész Tamás , Somogyi Zoltán , Balka Gyula
Published: January 2024

In the last few decades, many new paramyxoviruses have been discovered, causing diverse, mostly respiratory diseases in animals and humans. The porcine parainfluenza virus 1 (PPIV‐1, species Porcine respirovirus 1), which has been reported in many countries worldwide, was found in both healthy and clinically ill pigs showing respiratory signs. Here, we report the expected prevalence and genetic diversity of PPIV‐1 in Hungarian pig herds and the detection in one Slovakian pig farm, which is the first report of evidence for the presence of the virus in the country. To estimate the prevalence in Hungary 211 oral fluid samples were collected from 23 large‐scale swine herds in a systematic way and tested by real‐time quantitative RT‐PCR. The presence of the virus was detected in 10 of the 23 Hungarian farms (43%) included in our study. One hundred eighty‐one nasal swab samples were collected cross‐sectionally from three Hungarian and one Slovakian PPIV‐1‐positive herd and PPIV‐1 was most prevalent in 6‐week‐old pigs on farms located in Hungary and in the 2‐week‐old pigs on the Slovakian farm. Phylogenetic analysis of three Hungarian and two Slovakian PPIV‐1 F‐gene sequences showed high‐nucleotide identity (>93%) and all belonged to Clade I, together with the other European strains.
Parameter Control Framework for Multiobjective Evolutionary Computation Based on Deep Reinforcement Learning
Authors: Zhou Tianwei , Zhang Wenwen , Niu Ben , He Pengcheng , Yue Guanghui
Published: January 2024

To address the challenge of parameter adjustment in complex environments, this paper introduces a transfer learning-based parameter control framework via deep reinforcement learning for multiobjective evolutionary algorithms (MOEAs). To avoid the requirement for accurate Pareto front information, this framework is proposed with comprehensive global-state information, including basic problem features, the relative position of individuals, the distribution of fitness value, and the grid-IGD. Building on this framework, four reinforced multiobjective evolutionary algorithms (r-MOEAs) are proposed and tested on four DTLZ benchmarks and eight WFG benchmarks. The results of the comparative analyses reveal that compared with the original MOEAs, the four r-MOEAs exhibit faster convergence and stronger robustness. It is also confirmed that our proposed parameter control framework has the capability to learn knowledge from different experiences and improve the performance of MOEAs.
Cross‐Domain State‐of‐Charge Estimation of Li‐Ion Batteries Based on Source Domain Selection and Attention Mechanism
Authors: Zhang Wenliang , Hu Jingwei , Lin Bing , Liu Dui , Wang Mingfen , Mu Delong , Lin Chenye , Lu Yu
Published: January 2024

In the domain of lithium‐ion (Li‐ion) battery state‐of‐charge (SOC) estimation, deep neural network models commonly assume a congruent distribution between training and testing data. Nonetheless, this assumption often proves inadequate in real‐world scenarios, due to variations in environmental temperature, aging levels, and operational conditions. To tackle this challenge, this study proposes a novel approach centered around a deep transfer network, incorporating source domain selection and an attention mechanism, for the task of cross‐domain SOC estimation. This approach leverages a transfer network to extract bidirectional temporal features and accentuate salient information within sequences. The selection of an appropriate source domain for pretraining is contingent upon establishing domain similarity between the source and target domains. Experimental results demonstrate that the proposed method excels at feature extraction from Libs sequence data, yielding enhanced performance even when confronted with limited data.
Levetiracetam: A Potent Sword against Microglia Polarization in Gliomas
Authors: Cui Xiaoteng , Wang Qixue , Liu Xiaomin , Kang Chunsheng
Published: January 2024

Crosstalk between tumor cells and peritumoral cells contributes to immunosuppressive microenvironment formation in glioblastomas (GBM). A recent study revealed that glioma stem cells activated neuronal activity to promote microglial M2 polarization, leading to GBM progression, which could be pharmacologically blocked by levetiracetam, providing a practical strategy for GBM immunotherapy.

See related article by Guo et al., p. 1160
Supplementary Table 3 from SLFN11 is a BRCA Independent Biomarker for the Response to Platinum-Based Chemotherapy in High-Grade Serous Ovarian Cancer and Clear Cell Ovarian Carcinoma
Authors: Akashi Hidehiko , Yachida Nozomi , Ueda Haruka , Yamaguchi Manako , Yamawaki Kaoru , Tamura Ryo , Suda Kazuaki , Ishiguro Tatsuya , Adachi Sosuke , Nagase Yoshikazu , Ueda Yutaka , Ueda Masashi , Abiko Kaoru , Kagabu Masahiro , Baba Tsukasa , Nakaoka Hirofumi , Enomoto Takayuki , Murai Junko , Yoshihara Kosuke
Published: January 2024

Supplementary Table 3
Supplemental Table 1 from Bruton's Tyrosine Kinase Inhibitors with Distinct Binding Modes Reveal Differential Functional Impact on B-Cell Receptor Signaling
Authors: Li Wei , Sano Renata , Apatira Mutiah , DeAnda Felix , Gururaja Tarikere , Yang Muhua , Lundgaard Greta , Pan Chin , Liu Jing , Zhai Yongjiao , Yoon Woo Hyun , Wang Longcheng , Tse Chris , Souers Andrew J. , Lee Chih-Hung
Published: January 2024

Back-pocket and H3 pocket binders but not hinge-only binders inhibit calcium flux in Ramos and TMD8 cells
Supplementary Table S4 from MIG6 Mediates Adaptive and Acquired Resistance to ALK/ROS1 Fusion Kinase Inhibition through EGFR Bypass Signaling
Authors: Chen Nan , Tyler Logan C. , Le Anh T. , Welsh Eric A. , Fang Bin , Elliott Andrew , Davies Kurtis D. , Danhorn Thomas , Riely Gregory J. , Ladanyi Marc , Haura Eric B. , Doebele Robert C.
Published: January 2024

The complete list of identified phosphopeptides after global (pS/T/Y) phosphopeptide enrichment in crizotinib vs DMSO treated H3122 cells with fold changes and p-value calculation.
Table S1 from Delivery versus Potency in Treating Brain Tumors: BI-907828, a MDM2-p53 Antagonist with Limited BBB Penetration but Significant In Vivo Efficacy in Glioblastoma
Authors: Zhang Wenjuan , Vaubel Rachael A. , Oh Ju-Hee , Mladek Ann C. , Talele Surabhi , Zhang Wenqiu , Waller Katie L. , Burgenske Danielle M. , Sarkaria Jann N. , Elmquist William F.
Published: January 2024

Pharmacokinetics of BI-907828 in FVB mice in plasma and spinal cord after a single dose of 10 mg/kg
Figure S1 from Site-Specific Dolasynthen Antibody–Drug Conjugates Exhibit Consistent Pharmacokinetic Profiles across a Wide Range of Drug-to-Antibody Ratios
Authors: Clardy Susan M. , Uttard Alex , Du Bingfan , Catcott Kalli C. , Lancaster Kelly L. , Ditty Elizabeth , Sadowsky Jack , Zurita Jeffrey , Malli Naniye , Qin LiuLiang , Bradley Stephen P. , Avocetien Kenneth , Carter Tyler , Kim Dokyong , Nazzaro Mark , Xu Ling , Pillow Thomas H. , Zacharias Neelie T. , Lewis Gail D. , Rowntree Rebecca K. , Iyengar Radha , Lee David H. , Damelin Marc , Toader Dorin , Lowinger Timothy B.
Published: January 2024

Figure S1 shows SEC and HIC overlays for all ADCs compared to their parent antibodies
Data from Site-Specific Dolasynthen Antibody–Drug Conjugates Exhibit Consistent Pharmacokinetic Profiles across a Wide Range of Drug-to-Antibody Ratios
Authors: Clardy Susan M. , Uttard Alex , Du Bingfan , Catcott Kalli C. , Lancaster Kelly L. , Ditty Elizabeth , Sadowsky Jack , Zurita Jeffrey , Malli Naniye , Qin LiuLiang , Bradley Stephen P. , Avocetien Kenneth , Carter Tyler , Kim Dokyong , Nazzaro Mark , Xu Ling , Pillow Thomas H. , Zacharias Neelie T. , Lewis Gail D. , Rowntree Rebecca K. , Iyengar Radha , Lee David H. , Damelin Marc , Toader Dorin , Lowinger Timothy B.
Published: January 2024

Abstract
Key defining attributes of an antibody–drug conjugate (ADC) include the choice of the targeting antibody, linker, payload, and the drug‐to‐antibody ratio (DAR). Historically, most ADC platforms have used the same DAR for all targets, regardless of target characteristics. However, recent studies and modeling suggest that the optimal DAR can depend on target expression level and intratumoral heterogeneity, target internalization and trafficking, and characteristics of the linker and payload. An ADC platform that enables DAR optimization could improve the success rate of clinical candidates. Here we report a systematic exploration of DAR across a wide range, by combining THIOMAB protein engineering technology with Dolasynthen, an auristatin-based platform with monomeric and trimeric variants. This approach enabled the generation of homogeneous, site‐specific ADCs spanning a discrete range of DARs 2, 4, 6, 12, and 18 by conjugation of trastuzumab IgG1 THIOMAB constructs with 1, 2, or 3 engineered cysteines to monomeric or trimeric Dolasynthen. All ADCs had physicochemical properties that translated to excellent in vivo pharmacology. Following a single dose of ADCs in a HER2 xenograft model with moderate antigen expression, our data demonstrated comparable pharmacokinetics for the conjugates across all DARs and dose-dependent efficacy of all test articles. These results demonstrate that the Dolasynthen platform enables the generation of ADCs with a broad range of DAR values and with comparable physiochemical, pharmacologic, and pharmacokinetics profiles; thus, the Dolasynthen platform enables the empirical determination of the optimal DAR for a clinical candidate for a given target.
Data from Structural Insight into Geranylgeranyl Diphosphate Synthase (GGDPS) for Cancer Therapy
Authors: Pham Andrew C. , Holstein Sarah A. , Borgstahl Gloria E.O.
Published: January 2024

Abstract
Geranylgeranyl diphosphate synthase (GGDPS), the source of the isoprenoid donor in protein geranylgeranylation reactions, has become an attractive target for anticancer therapy due to the reliance of cancers on geranylgeranylated proteins. Current GGDPS inhibitor development focuses on optimizing the drug-target enzyme interactions of nitrogen-containing bisphosphonate-based drugs. To advance GGDPS inhibitor development, understanding the enzyme structure, active site, and ligand/product interactions is essential. Here we provide a comprehensive structure-focused review of GGDPS. We reviewed available yeast and human GGDPS structures and then used AlphaFold modeling to complete unsolved structural aspects of these models. We delineate the elements of higher-order structure formation, product-substrate binding, the electrostatic surface, and small-molecule inhibitor binding. With the rise of structure-based drug design, the information provided here will serve as a valuable tool for rationally optimizing inhibitor selectivity and effectiveness.
Supplementary Table.1 from The Tautomerase Activity of Tumor Exosomal MIF Promotes Pancreatic Cancer Progression by Modulating MDSC Differentiation
Authors: Jia Xuebing , Xi Jianbei , Tian Binle , Zhang Yuanyuan , Wang Zhilong , Wang Fan , Li Zheng , Long Jiang , Wang JianFei , Fan Guo-Huang , Li Qi
Published: January 2024

Antibodies for flow cytometry.
Supplementary Figure 2 from T cell–Dependent Bispecific Therapy Enhances Innate Immune Activation and Antibody-Mediated Killing
Authors: Besla Rickvinder , Penuel Elicia , Del Rosario Geoff , Cosino Ely , Myrta Szymon , Dillon Mike , Lazar Greg A. , Nickles Dorothee , Spiess Christoph , Yu Shang-Fan , Polson Andrew G.
Published: January 2024

NK-cell activation by TDB-induced killing increases expression of cytolytic genes.
Figure S4 from Tyrosine Kinase Inhibition Activates Intratumoral γδ T Cells in Gastrointestinal Stromal Tumor
Authors: Etherington Mark S. , Hanna Andrew N. , Medina Benjamin D. , Liu Mengyuan , Tieniber Andrew D. , Kwak Hyunjee V. , Tardy Katherine J. , Levin Lillian , Do Kevin J. , Rossi Ferdinando , Zeng Shan , DeMatteo Ronald P.
Published: January 2024

(A) Prevalence of IL17A+ γδ T cells in the spleens of KitV558Δ/+ mice after vehicle or imatinib treatment for 1 week. (B) Relative expression of Il6 by PCR of bulk KitV558Δ/+ tumors after 1 week of vehicle or imatinib. (C) Tumor weight in KitV558Δ/+ mice with 2 weeks of anti-IL23 therapy or isotype. Data are representative of 2 experiments each, 4 mice/experiment. Data represent mean ± SEM; p values were calculated using Student’s t test.
Supplementary Figure 6 from Spatial Distribution of Immune Cells Drives Resistance to Neoadjuvant Chemotherapy in Triple-Negative Breast Cancer
Authors: Donati Benedetta , Reggiani Francesca , Torricelli Federica , Santandrea Giacomo , Rossi Teresa , Bisagni Alessandra , Gasparini Elisa , Neri Antonino , Cortesi Laura , Ferrari Guglielmo , Bisagni Giancarlo , Ragazzi Moira , Ciarrocchi Alessia
Published: January 2024

Supplementary Figure 6. iTIL cell distribution according to NAC response in TNBC F-H) Staining of selective markers to detect the presence of CD20+ B lymphocytes (F), CD4+ T helper lymphocytes (G) and ERG+ endothelial cells (H) comparing pCR and pNR patients that had a similar percentage of sTILs at diagnosis. In each slide, an intra-epithelial area of the lesion is highlighted with higher magnification to show iTILs (left 5X magnification with scale bar 200µm; right 20X magnification with scale bar 50µm).
Data from T cell–Dependent Bispecific Therapy Enhances Innate Immune Activation and Antibody-Mediated Killing
Authors: Besla Rickvinder , Penuel Elicia , Del Rosario Geoff , Cosino Ely , Myrta Szymon , Dillon Mike , Lazar Greg A. , Nickles Dorothee , Spiess Christoph , Yu Shang-Fan , Polson Andrew G.
Published: January 2024

Abstract
T cell–retargeting therapies have transformed the therapeutic landscape for hematologic diseases. T cell–dependent bispecific antibodies (TDB) function as conditional agonists that induce a polyclonal T-cell response, resulting in target cell destruction and cytokine release. The relationship between this response and its effects on surrounding innate immune populations has not been fully explored. Here we show that treatment with mosunetuzumab in patients results in natural killer (NK) cell activation in the peripheral blood. We modeled this phenomenon in vitro and found that TDB-mediated killing activated NK cells, increasing NK function and antibody-dependent cellular cytotoxicity (ADCC), and enhanced the capability of macrophages to perform antibody-dependent cellular phagocytosis (ADCP). This enhancement was triggered by cytokines released through TDB treatment, with IL2 and IFNγ being major drivers for increased ADCC and ADCP, respectively. Surprisingly, cytolytic ability could be further augmented through neutralization of IL10 for NK cells and TNFα for macrophages. Finally, we showed that TDB treatment enhanced the efficacy of Fc-driven killing to an orthogonal solid tumor target in vivo. These results provide rationale for novel antibody therapy combinations that take advantage of both adaptive and innate immune responses.
Supplementary Figure S2 from Combined Inhibition of EZH2 and FGFR is Synergistic in BAP1-deficient Malignant Mesothelioma
Authors: Badhai Jitendra , Landman Nick , Pandey Gaurav Kumar , Song Ji-Ying , Hulsman Danielle , Krijgsman Oscar , Chandrasekaran Gayathri , Berns Anton , van Lohuizen Maarten
Published: January 2024

Supplementary Figure S2 shows efficient knock-down of BAP1 via inducible shRNA in uveal melanoma.
Supplementary Figure S1 from Combined Inhibition of EZH2 and FGFR is Synergistic in BAP1-deficient Malignant Mesothelioma
Authors: Badhai Jitendra , Landman Nick , Pandey Gaurav Kumar , Song Ji-Ying , Hulsman Danielle , Krijgsman Oscar , Chandrasekaran Gayathri , Berns Anton , van Lohuizen Maarten
Published: January 2024

Supplementary Figure S1 shows that knock-down of EZH2 sensitises Bap1-deficient mesothelioma cells to FGFR inhibition.
FIGURE 3 from Combined Inhibition of EZH2 and FGFR is Synergistic in BAP1-deficient Malignant Mesothelioma
Authors: Badhai Jitendra , Landman Nick , Pandey Gaurav Kumar , Song Ji-Ying , Hulsman Danielle , Krijgsman Oscar , Chandrasekaran Gayathri , Berns Anton , van Lohuizen Maarten
Published: January 2024

EZH2 inhibition together with FGFR inhibition is synergistic in uveal melanoma. A, Long-term clonogenicity assays of human uveal melanoma cell lines treated with DMSO, GSK126 (7.5 µmol/L), AZD4547 (0.75 µmol/L), or the combination. B, Similar to the data shown in A, the clinically available inhibitor tazemetostat (10 µmol/L) was used as an EZH2 inhibitor instead of GSK126. C, Clonogenicity assays of the uveal melanoma cell line with inducible shRNA against BAP1 or shRANDOM treated with DMSO, GSK126 (7.5 µmol/L), tazemetostat (10 µmol/L), AZD4547 (0.75 µmol/L), or a combination of the EZH2 inhibitor and FGFR inhibitor; Representative images are shown from three independent experiments, images of control wells are duplicated for presentation purposes,. Quantification data are mean ± SEM, n = 3 independent experiments, P values were determined by using two-tailed unpaired Student t test; ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001.
FIGURE 2 from Combined Inhibition of EZH2 and FGFR is Synergistic in BAP1-deficient Malignant Mesothelioma
Authors: Badhai Jitendra , Landman Nick , Pandey Gaurav Kumar , Song Ji-Ying , Hulsman Danielle , Krijgsman Oscar , Chandrasekaran Gayathri , Berns Anton , van Lohuizen Maarten
Published: January 2024

EZH2 inhibition results in upregulation of FGFR pathways. A, GO-term analysis on differentially expressed genes between BNC cell lines (n = 2) that were treated with GSK126 or untreated. B, KEGG pathway enrichment analysis on samples mentioned in A. C, Long-term clonogenicity assays of human mesothelioma cell lines treated with DMSO, GSK126 (7.5 µmol/L), BEZ-235 (15 nmol/L), or the combination. Representative images are shown from two independent experiments.
Parapneumonischer Pleuraerguss bei ambulant erworbener Pneumonie: Welcher Patient ist «at risk»?
Authors: Keymel Stefanie
Published: January 2024

Background: Community-acquired pneumonia (CAP) patients usually present with parapneumonic pleural effusion (PPE), which complicates the treatment of pneumonia. This study aims to investigate the clinical characteristics and risk factors of elderly CAP patients hospitalised with PPE. Methods: The clinical data of 132 elderly patients with CAP were retrospectively analysed. A total of 54 patients with PPE (PPE group) and 78 patients without PPE (NPPE group) were included in this study. Clinical data, laboratory examinations, treatments and other relevant indicators were collected. Univariate analysis and multivariate logistic regression analysis will be used to explore the possible risk factors for PPE. Results: The proportion of PPE in elderly patients with CAP was 40.9%. PPE patients were significantly more likely to be older, have comorbid neurological diseases, experience chest tightness, and have a lasting fever (P< 0.05). In contrast to NPPE patients, the total number of lymphocytes, serum albumin and blood sodium levels in the PPE group were significantly lower (P < 0.05). The blood D-dimer, C-reactive protein and CURB-65 score of PPE patients were significantly higher (P < 0.05) than those of NPPE patients. Multivariate logistic regression identified chest tightness (OR = 3.964, 95% CI: 1.254–12.537, P = 0.019), long duration of fever (OR = 1.108, 95%CI: 1.009–1.217, P = 0.03), low serum albumin (OR = 0.876, 95%CI: 0.790– 0.971, P = 0.012) or low blood sodium (OR = 0.896, 95%CI: 0.828–0.969, P= 0.006) as independently associated with the development of parapneumonic pleural effusion in the elderly.
Hepatocellular Carcinoma: The Role of Surgery in Liver Cirrhosis
Authors: Koch Dominik T. , Horné Fabian , Fabritius Matthias P. , Werner Jens , Ilmer Matthias
Published: January 2024

Background: Liver surgery is an essential component of hepatocellular carcinoma (HCC) treatment. Advances in surgical techniques and perioperative care have improved outcomes and have helped to expand surgical indications. However, liver fibrosis and cirrhosis still remain major problems for liver surgery due to the relevant impact on liver regeneration of the future liver remnant (FLR) after surgery. Especially in patients with clinically significant portal hypertension due to liver cirrhosis, surgery is limited. Despite recent efforts in developing predictive models, estimating the postoperative hepatic function remains difficult. Summary: In this review, we focus on the role of surgery in the treatment of HCC in structurally altered livers. The importance of assessing FLR with techniques such as contrast-enhanced CT, e.g., with the help of artificial intelligence is highlighted. Moreover, strategies for increasing the FLR with approaches like portal vein embolization and liver vein deprivation prior to surgery are discussed. Patient selection, minimally invasive liver surgery including robotic techniques, and perioperative concepts like the Enhanced Recovery After Surgery (ERAS) guidelines are identified as crucial parts of avoiding posthepatectomy liver failure. Key Message: The need for ongoing research to optimize patient selection criteria and perioperative care and to develop innovative biomarkers for outcome prediction is emphasized.
Cultivating The Research Culture Mediated by Motivation Factors in Enhancing Teachers’ Performance in Conducting Action Research
Authors: Esturas Maricel D.
Published: January 2024

Nowadays, the Department of Education recently displayed an anticipated and overwhelming interest in developing a research culture. DepEd promoted action research and encouraged teachers to engage, thus this is timely to address the learning gaps during pandemic. With this set up, teachers ought to be involved in it to foster evidence-based practice and raise research productivity. The major objective of this study was to ascertain how the research culture and motivating elements of action research to improve teachers' research performance in the selected elementary school.

The study was conducted at sixty-two schools in the division of San Pablo City using the ex post facto research design. A total of 750 elementary teachers randomly selected comprised the respondents of the study.

The correlation between motivational factors and research skills and research productivity of teachers were all significantly related. The highest correlation in terms of data analysis can be seen evidently in personal satisfaction. This reveals that teachers engaged in research activities when chances are given to utilize their skills and the administration supports the presentation of research papers. The performance and research productivity of the teachers' research abilities and efforts have a significant indirect impact on the motivational variables involved in the research. The end result of this study signifies that the relationship between the research organizational culture and teacher research performance could be enhanced by improving the motivational factors and research skills.
Effective Leadership and Supervisory Skills of Principal: Predictors of Job Satisfaction among Public Elementary School Teachers
Authors: Beladas Jean P. , Callo Eden C.
Published: January 2024

The study's dependent variable is the teacher's compelling desire to know the Degree of job satisfaction in terms of fundamental requirements, self-actualization, supervision, and indicators. Job safety, predictable income, vessel maintenance and equipment, mental stress on the job, your earnings or Pay, cleanliness of the work, hours worked, peace of mind/security, time away from home, healthfulness of the workplace, and physical exhaustion on the job are all necessities. Accountability, productivity, recognition, job challenge, excitement, and undertaking something worthwhile are all components of self-actualization. The study on principals' effective leadership and supervisory abilities as predictors of work satisfaction among teachers in public primary schools seeks to determine whether the two qualities of the principal have anything to do with teachers' passion for their jobs. The study uses additional supporting material to demonstrate the relationship between leadership, supervisory skills, and job happiness. The respondents strongly agreed with the claims about their respective public-school principals' leadership abilities, demonstrated effective supervisory skills in their respective schools, improved those institutions, and produced outstanding students. The findings suggest a strong relationship between teachers' job satisfaction and the principal's leadership abilities. Furthermore, teacher job satisfaction and the effectiveness of the principal's supervision are highly correlated.
Exploring The Factors Influencing Learners’ Attitudes and Engagement Towards Learning Outcomes in Araling PanlipunanAmong Grade 10 Learners
Authors: Gonzales Bernadette T. , Hermosa Jherwin P.
Published: January 2024

his study aimed to discern the factors influencing attitudes and engagement towards learning Araling Panlipunan in terms of instructional methods, classroom environment, the difficulty of the subject, teachers' behavior, learning resources, teaching competency, learning style, and integration of technology. Moreover, it also determined the level of the learning outcomes in Araling Panlipunan as cognitive, behavioral, and attitudinal. The study utilized a descriptive research design to collect quantifiable information that can be used to make statistical inferences about the target audience through data analysis. Stratified Random Sampling was used to identify 127 grade 10 learners from San Isidro Integrated National Highschool in the Schools Division of Lipa City. The data were gathered using a survey questionnaire which was validated with the help of the experts.

Findings revealed that there is a significant relationship between instructional methods and the learning outcomes in Araling Panlipunan as to Cognitive, Behavioral, and Attitudinal.  The classroom environment was found to have highly significant relationships with cognitive, behavioral, and attitudinal learning outcomes in Araling Panlipunan. The difficulty of the subject has a highly significant relationship with cognitive and attitudinal learning outcomes.  As for teacher behavior, highly significant relationships were recognized about cognitive, behavioral, and attitudinal Learning Outcomes in Araling Panlipunan. However, after all the data was gathered and calculated, the result revealed that there is no significant relationship between the Difficulty of the Subject and Behavioral Learning Outcomes in Araling Panlipunan.
Teacher-Mediated Pronunciation Instruction: Its Effects on Enhancing the Oral Language Fluency of Seventh-Grade English Students
Authors: Barrun Jason S. , Sia Jonald B.
Published: January 2024

This study investigated the effects of teacher-mediated pronunciation instruction on enhancing the oral language fluency of seventh-grade English students. Using a quasi-experimental pretest-posttest non-equivalent groups design, 32 learners served as respondents of the thirteen-session- study in a secondary school in Southern Luzon, Philippines. The student-respondents were tested to find out whether there would be a significant difference in their knowledge and application of the segmentals and suprasegmentals before and after the implementation of the said intervention.

 In all the instruments, namely the dictation test, pronunciation achievement test, and aural-oral achievement test, the findings revealed that the experimental group performed significantly higher than the control group. Through the independent sample t-test and Analysis of Covariance (ANCOVA), the results revealed that the experimental group had higher mean scores than the control group with t-computed values exceeding the tabular values affirming that an explicit pronunciation instruction is better in enhancing the oral language fluency of students than only incidental acquisition. The more the learners are exposed to an intervention, the more that they will become intelligible. Other effects also include the more frequently that the teacher conducts various games, the more that the students enjoy and learn pronunciation; and the more refined a learner’s pronunciation skill is, the more improved he/she is when it comes to listening comprehension and spelling. Finally, it is recommended that more and newer interventions regarding second language phonology or any content that focuses on speaking as a macro-strand in English should be delved into.
Disparities by Sex, Race, and Ethnicity in Use of Left Ventricular Assist Devices and Heart Transplants Among Patients With Heart Failure With Reduced Ejection Fraction
Authors: Rose Scott W. , Strackman Braden W. , Gilbert Olivia N. , Lasser Karen E. , Paasche‐Orlow Michael K. , Lin Meng‐Yun , Saylor Georgia , Hanchate Amresh D.
Published: January 2024

Background

The extent to which sex, racial, and ethnic groups receive advanced heart therapies equitably is unclear. We estimated the population rate of left ventricular assist device (LVAD) and heart transplant (HT) use among (non‐Hispanic) White, Hispanic, and (non‐Hispanic) Black men and women who have heart failure with reduced ejection fraction (HFrEF).

Methods and Results

We used a retrospective cohort design combining counts of LVAD and HT procedures from 19 state inpatient discharge databases from 2010 to 2018 with counts of adults with HFrEF. Our primary outcome measures were the number of LVAD and HT procedures per 1000 adults with HFrEF. The main exposures were sex, race, ethnicity, and age. We used Poisson regression models to estimate procedure rates adjusted for differences in age, sex, race, and ethnicity. In 2018, the estimated population of adults aged 35 to 84 years with HFrEF was 69 736, of whom 44% were women. Among men, the LVAD rate was 45.6, and the HT rate was 26.9. Relative to men, LVAD and HT rates were 72% and 62% lower among women (
P
<0.001). Relative to White men, LVAD and HT rates were 25% and 46% lower (
P
<0.001) among Black men. Among Hispanic men and women and Black women, LVAD and HT rates were similar (
P
>0.05) or higher (
P
<0.01) than among their White counterparts.

Conclusions

Among adults with HFrEF, the use of LVAD and HT is lower among women and Black men. Health systems and policymakers should identify and ameliorate sources of sex and racial inequities.
Devices of Articulation: Who Ever Said They Have to Be Smart?
Authors: Kolks Laurens
Published: January 2024

In this article, I argue to expand the application of the concept “devices of articulation”—a term signifying those artifacts that are purposefully created to articulate public issues: controversial phenomena that are too important not to be considered by designers but are not necessarily solvable by political or scientific means. Whereas problems might be fixed, issues can only be temporarily stabilized. I, therefore, investigate how two design projects—Smogware and the Rain Project—forge new relations, meanings, and consequences among elements that are typically understood to be unrelated, to support public engagement with the issue of environmental pollution.
Rule by Metrics
Authors: Infantino Marta , Bussani Mauro
Published: January 2024

We live in societies in which an ever-growing array of activities in a range of fields are assessed, controlled and governed according to quantitative techniques. This paper focuses on processes grounded on quantitative tools (e.g. scores, indicators, rankings, algorithms) that are used in order to measure the performance of processes, people or organizations. Such processes are referred to in this paper as ‘legal metrics’. We explore how different legal systems and sectors rely on legal metrics, and develop a twofold argument. On the one hand, we argue that performance-based quantification often qualifies as a form of regulatory intervention. On the other hand, we contend that performance-based measures are always adopted and applied in legal contexts that react differently to the quantification turn. Both sides of this picture have largely been overlooked by comparative lawyers, even though they imply looking for law in extraordinary places and appraising a variety of legal manifestations in different settings. Understanding which forms of quantitative measures are widespread, in which sectors and regions, made by whom and producing what regulatory effects, is a research task that could (and in our opinion should) be of interest for all comparative law scholars.
A new endangered species of Caperonia (Acalyphoideae, Euphorbiaceae) from South Brazil, with morphological and phylogenetic support
Authors: KÜLKAMP JOSIMAR , VALDUGA EDUARDO , IGANCI JOÃO R.V.
Published: January 2024

Caperonia (Acalyphoideae, Euphorbiaceae) is a monophyletic genus with 36 species naturally distributed in Latin America, Africa, and Madagascar. Its greatest diversity occurs in South America, where 28 species are found. Caperonia species are herbs or subshrubs that occur exclusively in open marshy environments. Here, we describe Caperonia itapevae, a new endangered species known from a single record from South Brazil, based on extensive morphological study and phylogenetic data.
Gentiana betongensis (Gentianaceae), a new species from tropical montane sphagnum bog in Peninsular Thailand
Authors: WAI JAREARNSAK SAE
Published: January 2024

Gentiana betongensis is described as a new species from Thailand. It was discovered in tropical montane sphagnum bog of the High Plateau in Betong District, Yala Province near the Thai-Malay Border. The new species is morphologically most similar to G. malayana in having erect, simple or few-branched stems, but differs markedly in having smaller flowers, triangular-ovate, acute corolla lobes, and long, recurved and coiled stigma lobes. This paper provides morphological description, illustration, photographs, distribution map, ecological data, reproductive biology, DNA barcode sequence, vernacular name, conservation status, a key to the species of Gentiana in Thailand, Peninsular Malaysia and Borneo, and a special note on conservation perspectives.
Pestalotiopsis phyllostachydis sp. nov. from Guangdong, China
Authors: ZHAO HAIJUN , SHU YONGXIN , DOILOM MINGKWAN , ZENG XIANGYU , ZHANG HUANG , DONG WEI
Published: January 2024

Pestalotiopsis phyllostachydis sp. nov. is introduced during a study of the diversity of bambusicolous fungi in Guangdong Province, China. The new species exhibits similar conidial morphology, pigmentation, and dimensions to its relatives; however, it can be distinguished by the length of its apical and basal appendages. The identification is corroborated by a phylogenetic analysis incorporating combined ITS, tef1-α and tub2 sequence data. A refined description and illustration of P. phyllostachydis are provided, accompanied by a morphological comparison of this new taxon and its closely related taxa compiled in a table. In addition, the common morphological characteristics utilized for interspecific identification are discussed incidentally.
Notes on the genus Dictyogenus Klapálek, 1904 (Plecoptera, Perlodidae) in the Austrian, Italian, Slovenian and Swiss Alps, with the description of six new species
Authors: REDING JEAN-PAUL G.
Published: January 2024

Six new species of Dictyogenus Klapálek, 1904, from the Austrian, Italian, Slovenian, and Swiss Alps, are described and illustrated from adults and nymphs. The species Dictyogenus alpinum (Pictet, 1841) and topotypical Dictyogenus fontium (Ris, 1896) are re-described.
New species of the elusive crickets from the genus Pendleburyella Chopard, 1969 (Gryllidae, Pentacentrinae) from Sabah, Borneo
Authors: TAN MING KAI , JAPIR RAZY , CHUNG ARTHUR Y.C.
Published: January 2024

Only one species of Pendleburyella Chopard, 1969 (Gryllidae, Pentacentrinae) was previously known to occur in Borneo: Pendleburyella eirmosa Tan, Muhammad & Wahab, 2023 from Brunei Darussalam. Here, two additional new species of crickets from the genus Pendleburyella are described from the highlands of Sabah, Borneo: Pendleburyella eremita sp. nov. from Bukit Hampuan and Pendleburyella nimba sp. nov. from Mount Trus Madi. In total, six species of Pendleburyella are currently known, three from Borneo and three from Malay Peninsula. It is likely that many more species are awaiting discovery owing to its cryptic morphology and elusive nature.
Lineagona, a new millipede genus (Diplopoda, Chordeumatida, Cleidogonidae) from Texas, USA, and Nuevo León, México
Authors: SHEAR WILLIAM A.
Published: January 2024

Lineagona n. gen., is established for two species from either side of the Rio Grande River in Texas and México. Lineagona culmenicola n. sp., is from the vicinity of Monterrey, Nueva Leon, México, and L. chisosi (Loomis, 1963) n. comb. ex Cleidogona Cook, in Cook & Collins 1895 is from Big Bend National Park, Texas, USA. A key to the genera of Cleidogonidae is provided and gonopod terminology in Cleidogonidae is updated.