bluuebunny/crossref_metadata_2025 huggingface dataset:
Highs and lows of sympathetic neurocardiovascular transduction: influence of altitude acclimatization and adaptation
Authors: Berthelsen Lindsey F , Fraser Graham M. , Simpson Lydia L. , Vanden Berg Emily R. , Busch Stephen A. , Steele Andrew R. , Meah Victoria L. , Lawley Justin S. , Figueroa-Mujíca Romulo J. , Vizcardo-Galindo Gustavo , Villafuerte Francisco , Gasho Chris , Willie Christopher K. , Tymko Michael M. , Ainslie Philip N. , Stembridge Mike , Moore Jonathan P. , Steinback Craig D.
Published: September 2020

This study has identified that sympathetically mediated blood pressure regulation is reduced following ascent to high altitude. Additionally, we show that high-altitude Andean natives have reduced blood pressure responsiveness to sympathetic nervous activity (SNA) outflow compared with Nepalese Sherpa. However, basal sympathetic activity is inversely related to the magnitude of SNA-mediated fluctuations in blood pressure regardless of population or condition. These data set a foundation to explore more precise mechanisms of blood pressure control under conditions of persistent sympathetic activation and hypoxia.
Cluster Optimization in Mobile Ad Hoc Networks Based on Memetic Algorithm: memeHoc
Authors: Ahmad Masood , Hameed Abdul , Ullah Fasee , Khan Atif , Alyami Hashem , Uddin M. Irfan , ALharbi Abdullah , Aziz Furqan
Published: September 2020

In mobile ad hoc networks (MANETs), the topology differs very often due to mobile nodes (MNs). The flat network organization has high topology maintenance messages overload. To reduce this message overload in MANET, clustering organizations are recommended. Grouping MANET into MNs has the advantage of controlling congestion and easily repairing the topology. When the MANET size is large, clustered MN partitioning is a multiobjective optimization problem. Several evolutionary algorithms such as genetic algorithms (GAs) are used to divide MANET into clusters. GAs suffer from premature convergence. In this article, a clustering algorithm based on a memetic algorithm (MA) is proposed. MA uses local exploration techniques to reduce the likelihood of early convergence. The local search function in MA is to find the optimal local solution before other evolutionary algorithms. The optimal clusters in MANET can be achieved using MA for dynamic load balancing. In this work, the network is considered a graph G (V, E), where V represents MN and E represent the communication links of the neighboring MNs. The aim of this study is to find the cluster headset (CH) as early as possible when needed. High-quality individuals are selected for the new population in the next generation. New individuals are generated using the crossover mechanism on the chromosome once the two parents have been selected. Data are communicated via CHs between other clusters. The proposed technique is compared with existing techniques such as DGAC, MobHiD, and EMPSO. The proposed technique overcomes the state-of-the-art clustering schemes in terms of cluster counting, reaffiliation rate, cluster life, and overload of control messages.
The Relationship between Symptoms and Social Functioning over the Course of Cognitive Behavioral Therapy for Social Anxiety Disorder
Authors: Ogawa Sei , Imai Risa , Suzuki Masako , Furukawa Toshi A. , Akechi Tatsuo , Ferraro Luca
Published: September 2020

Background. The present study is aimed at investigating the relationship between changes in symptoms and changes in social functioning during cognitive behavioral therapy (CBT) for social anxiety disorder (SAD). Methods. Ninety-six patients with SAD were treated with manualized group CBT. Measures of social anxiety symptoms, depression symptoms, cognition, and social functioning were administered at baseline and endpoint. Using multiple regression analysis, we examined the associations between the changes in four aspects (work, home management, social leisure activities, and private leisure activities) of social functioning as dependent variables and the changes in four factors (social interaction, public speaking, observation by others, and eating and drinking in public) in social anxiety symptoms, depression symptoms, and cognition as independent variables. Results. The changes in work functioning were predicted by the changes in the public speaking factor in social anxiety symptoms. The changes in depression symptoms predicted the changes in home management. The significant predictors of changes in social leisure activities were the changes in the social interaction factor and depression symptoms. The changes in private leisure activities were predicted by the changes in the observation by others factor. The changes in cognition predicted nothing. Conclusion. The present study suggested that the changes in social anxiety or depression symptoms may predict several aspects of social functioning changes in patients with SAD over the course of CBT. In order to improve social functioning, our results may be useful for selecting the fear or feared situation in CBT for SAD. Trial Registration. The clinical study registration number in the Japanese trials registry is UMIN CTR 000031147.
Influence of Global Spine Sagittal Balance and Spinal Degenerative Changes on Locomotive Syndrome Risk in a Middle‐Age and Elderly Community‐Living Population
Authors: Machino Masaaki , Ando Kei , Kobayashi Kazuyoshi , Nakashima Hiroaki , Kanbara Shunsuke , Ito Sadayuki , Inoue Taro , Yamaguchi Hidetoshi , Koshimizu Hiroyuki , Seki Taisuke , Ishizuka Shinya , Takegami Yasuhiko , Ishiguro Naoki , Hasegawa Yukiharu , Imagama Shiro
Published: September 2020

Purpose. The aim of this study was to describe the characteristics of each locomotive syndrome (LS) risk stage, including global spine sagittal alignment, spinal degenerative changes evident on plain radiographs, low back pain (LBP), muscle strength, and physical ability in middle‐aged and elderly people in a health checkup. Methods. This study included 211 healthy Japanese volunteers (89 men and 122 women; mean age, 64.0 years) who underwent assessment with both radiographs and Spinal Mouse. Spinal sagittal parameters included thoracic kyphosis angle (TKA), lumbar lordosis angle (LLA), sagittal vertical axis, and spinal inclination angle (SIA). Lumbar disc height (LDH) and lumbar osteophyte formation (LOF) at each level were evaluated as the spinal degenerative changes. The LS assessment comprised three tests: stand‐up test, two‐step test, and 25‐question Geriatric Locomotive Function Scale (GLFS‐25). The subjects were divided into three groups (no risk, stage 1 LS, or stage 2 LS) according to LS risk test criteria. The prevalence of LBP was investigated with a visual analogue scale (VAS), and physical performances were also compared among the groups. Results. Of the participants, 122 had no risk of LS, 56 had stage 1 LS risk, and 29 had stage 2 LS risk. With increasing LS risk stage, the prevalence of and VAS score for LBP increased significantly, and back muscle strength and physical abilities decreased significantly. The TKA did not differ among the three groups. The LLA decreased gradually with LS risk stage (P = 0.0001). At each level except L1–L2 and L5–S1, LDH decreased gradually with LS risk stage. The prevalence of LOF increased significantly with increasing LS risk stage. The SIA increased significantly with LS risk stage (P = 0.0167). Conclusions. Participants with LS had higher prevalence of spinal degeneration, small LLA, and global spinal imbalance by anterior spinal inclination.
Comparative Study of the Effects of 1% Atropine on the Anterior Segment
Authors: Zhou Yue , Huang Xiao Bo , Cai Qi , Li Jun Jie , Xiong Yao Jia , Gong Ye Xun , Lin Li Wei , Zhu Yan , Sun Zhi Min , Mwanza Jean-Claude
Published: September 2020

Purpose. To investigate the influences of atropine on changes in anterior segment geometry, as measured by ultrasound biomicroscopy in children. Methods. A prospective observational study was performed. Anterior segment parameters were obtained by UBM before and after the instillation of 1% atropine. Univariate linear regression was performed to identify the variables contributing to the changes in the trabecular meshwork-iris angle (TIA). Results. The study included 21 boys and 37 girls with a mean age of 10.79 ± 2.53 years. Anterior chamber parameters including the central anterior chamber depth, TIA, angle opening distance at 500 μm from the scleral spur, iris thickness 750 μm and 1500 μm from the scleral spur, trabecular-ciliary angle (TCA), trabecular-ciliary process distance, sclera-iris angle (SIA), and sclera-ciliary process angle significantly increased after cycloplegia (

P
<
0.05

). In contrast, the lens vault, iris cross-sectional area, and maximum ciliary muscle thickness significantly decreased after cycloplegia. Univariate analysis identified the change in TCA and the change in SIA and the TIA before mydriasis as determinants of the change in TIA. Conclusions. Atropine causes statistically significant changes in various anterior segment parameters in children. The change in anterior chamber angle is associated with the change in TCA and the change in SIA and the TIA before mydriasis.
Screening of 22q11.2DS Using Multiplex Ligation‐Dependent Probe Amplification as an Alternative Diagnostic Method
Authors: Maran Sathiya , Faten Siti Aisyah , Lim Swee-Hua Erin , Lai Kok-Song , Ibrahim Wan Pauzi Wan , Ankathil Ravindran , Gan Siew Hua , Tan Huay Lin
Published: September 2020

Background. The 22q11.2 deletion syndrome (22q11.2DS) is the most common form of deletion disorder in humans. Low copy repeats flanking the 22q11.2 region confers a substrate for nonallelic homologous recombination (NAHR) events leading to rearrangements which have been reported to be associated with highly variable and expansive phenotypes. The 22q11.2DS is reported as the most common genetic cause of congenital heart defects (CHDs). Methods. A total of 42 patients with congenital heart defects, as confirmed by echocardiography, were recruited. Genetic molecular analysis using a fluorescence in situ hybridization (FISH) technique was conducted as part of routine 22q11.2DS screening, followed by multiplex ligation‐dependent probe amplification (MLPA), which serves as a confirmatory test. Results. Two of the 42 CHD cases (4.76%) indicated the presence of 22q11.2DS, and interestingly, both cases have conotruncal heart defects. In terms of concordance of techniques used, MLPA is superior since it can detect deletions within the 22q11.2 locus and outside of the typically deleted region (TDR) as well as duplications. Conclusion. The incidence of 22q11.2DS among patients with CHD in the east coast of Malaysia is 0.047. MLPA is a scalable and affordable alternative molecular diagnostic method in the screening of 22q11.2DS and can be routinely applied for the diagnosis of deletion syndromes.
Effect of Plant Spacing on Yield and Yield Components of Tomato (Solanum lycopersicum L.) in Shewarobit, Central Ethiopia
Authors: Amare Getachew , Gebremedhin Hailay
Published: September 2020

Inappropriate spacing is one of the major problems in tomato production at the study area. A field experiment was conducted to determine inter- and intrarow plant spacing for yield and yield components of tomato at Shewarobit, central rift valley of Ethiopia, under irrigation condition. The treatment comprises of three intrarow spacing (20, 30, and 40 cm) and four interrow spacing (60, 80, 100, and 120 cm) replicated three times and arranged in randomized complete block design using tomato variety Weyno. Data collected on fruit yield and yield components were analysed using SAS. The main effect of interrow spacing significantly affected marketable fruit, unmarketable fruit, marketable fruit number, unmarketable fruit number, and fruit diameter. The 20 cm interrow spacing showed a marked increase in marketable fruit yield by 35.96% as compared to 30 cm spacing used by farmers. Planting tomato in closer interrow spacing (60 cm) resulted in 50% yield increment than the widest (120 cm) space between rows. Interaction effects of both inter- and intrarow spacing significantly (p < 0.05) affected plant height and fruit length. An intrarow and interrow spacing of 20 ∗ 100 cm and 20 ∗ 120 cm resulted in tallest plants and widest fruits, respectively. Therefore, farmers can use 20 cm intrarow spacing and 60 cm interrow spacing for planting of tomato seedling of Weyno variety.
Headache in Patients with Cervical Spondylotic Myelopathy
Authors: Yabuki Shoji , Takatsuki Kozue , Otani Koji , Nikaido Takuya , Watanabe Kazuyuki , Kato Kinshi , Kobayashi Hiroshi , Handa Jun-ichi , Konno Shinichi , Antonaci Fabio
Published: September 2020

Purpose. The anatomical mechanisms of cervicogenic headache caused by upper cervical lesions have been reported. However, the pathomechanisms of headache caused by lower cervical spine disorders remain unknown. The purpose of the current study was to clarify the prevalence and pathogenesis of headaches in patients with cervical spondylotic myelopathy (CSM). Methods. In this retrospective study, a questionnaire regarding preoperative and postoperative symptoms was sent to 147 patients with CSM who were surgically treated in our hospital during the previous 10 years. All of the surgical procedures were decompression surgeries between the C3 and C7 levels. Data from 74 patients (50.3%) were available for analysis. Subjects were divided into four groups according to the presence or absence of preoperative and postoperative headache. The severity of pain, severity of neuropathic pain symptoms, depression, severity of myelopathy, and quality of life (QOL) were also evaluated using questionnaires. The scores of these questionnaires were then compared between the four groups. Kruskal–Wallis tests with Dunn–Bonferroni post hoc tests were used for comparisons. Results. Of the patients with CSM, 31% had headaches preoperatively, and 43% of these headaches disappeared postoperatively. Type 4 (preoperative headache-positive/postoperative headache-positive) patients had more severe pain and neuropathic pain symptoms and lower QOL scores compared with type 1 (preoperative headache-negative/postoperative headache-negative) patients. Conclusions. Approximately one-third of all patients with CSM had headaches preoperatively. Headache in patients with CSM may be neuropathic pain. A proportion of headaches in patients with CSM can be treated by decompression surgery.
Energy-Efficient Asynchronous QoS MAC Protocol for Wireless Sensor Networks
Authors: Sarang Sohail , Stojanović Goran M. , Stankovski Stevan , Trpovski Željen , Drieberg Micheal
Published: September 2020

In recent years, wireless sensor networks (WSNs) have gained significant attention in both industry and academia. In WSNs, each sensor node is normally equipped with a small-size battery with finite capacity. Hence, energy-efficient communication is considered a key factor for the extension of network lifetime. Formerly, a large number of medium access control (MAC) protocols have been proposed to improve energy efficiency to prolong the network lifetime. There are applications that generate different types of data packets and require quality of service (QoS) without any disruption in network operation. Therefore, these applications need an energy-efficient QoS MAC protocol that can support QoS by considering energy efficiency as the primary goal to avoid any failure in the network. This article proposes an energy-efficient asynchronous QoS (AQSen) MAC protocol, called AQSen-MAC. The AQSen-MAC considers different types of data packets and uses two novel techniques: self-adaptation and scheduling to enhance energy efficiency, packet delivery ratio, and network throughput. Furthermore, in the protocol, the receiver adjusts its duty cycle according to the remaining energy to prolong the network operation. Finally, the performance of the AQSen-MAC protocol has been evaluated through detailed simulation using Castalia and compared with MPQ-MAC, PMME-MAC, and QAEE-MAC protocols. The simulation results indicate that the AQSen-MAC protocol significantly reduces the energy consumption at the receiver of up to 13.4%, consumption per bit of up to 3% and improves the packet delivery ratio and network throughput of up to 12% in the network.
Effectiveness of Low‐Frequency Stimulation in Proprioceptive Neuromuscular Facilitation Techniques for Post Ankle Sprain Balance and Proprioception in Adults: A Randomized Controlled Trial
Authors: Alahmari Khalid A. , Silvian Paul , Ahmad Irshad , Reddy Ravi Shankar , Tedla Jaya Shanker , Kakaraparthi Venkata Nagaraj , Rengaramanujam Kanagaraj
Published: September 2020

Stretching is an important part of post ankle sprain rehabilitation, as well as an effective exercise for improving general ankle‐joint performance. But the combination of stretching alongside low‐frequency stimulation has not yet been extensively studied. Therefore, the purpose of the present randomized controlled trial was to compare the combined effects of low‐frequency transcutaneous electrical nerve stimulation (TENS) with proprioceptive neuromuscular facilitation (PNF) on strength, balance, and proprioception among individuals with post ankle sprain. Sixty male subjects with lateral ankle sprain were selected and randomly allocated to three groups: group 1, group 2, and the control group (CG). Subjects in group 1 received the PNF stretching technique combined with TENS. TENS stimulation was provided using two electrodes placed 5 cm apart directly on the triceps sural muscle of the affected leg and a biphasic current with a symmetrical waveform at 50 Hz for 15 seconds, tuned for a 3‐second ramp up time and a 30‐second rest time with a 250‐microsecond pulse duration was given with PNF stretching. Subjects in group 2 received the PNF stretching technique alone. Both group 1 and group 2 received these treatments for 4 weeks (4 days/week); follow‐up assessments were administered in the third and fifth weeks. CG received no treatment; outcome measures alone were assessed. Outcome measures comprised pain, balance, flexibility, proprioception, range of motion, muscle strength, and functional limitation. A mixed‐model ANOVA showed significant interaction (time and group) and the time effect for all the outcome measures (p ≤ 0.05). Group 1 (PNF‐TENS) showed significant improvement for all the outcome variables compared to the other groups. The present study showed PNF stretching combined with TENS for the triceps sural muscle to trigger muscle contraction during the muscle contraction phase of the PNF stretch, compared against PNF stretching alone, produced significant improvements in ankle function for post ankle sprain subjects.
Determinants of Implanon Discontinuation among Women Who Use Implanon at Bahir Dar Town Health Institutions, Northwest Ethiopia, 2019: A Case‐Control Study
Authors: Yehuala Tsegaw , Melese Ergoye , Bogale Kassawmar Angaw , Dagnew Baye
Published: September 2020

Background. Implanon is a long‐acting reversible contraceptive method that is 99% effective in preventing unintended pregnancy. Despite its effectiveness, the rate of Implanon discontinuation is high. In Ethiopia, there is limited information about determinants of Implanon discontinuation. Therefore, this study aimed to identify the determinants of Implanon discontinuation among women who used Implanon at Bahir Dar town health institutions. Methods. We employed an unmatched case‐control study to find out the determinants of Implanon discontinuation at Bahir Dar town health institutions from March to June 2019 using the multistage stratified sampling technique to select study participants. Cases were women who had discontinued Implanon before completion of 3 years, and controls were women who had removed Implanon at the date of appointment (3 years). A pretested, structured questionnaire with face‐to‐face interviews was used. Binary logistic regression was performed to identify determinants of Implanon discontinuation. In the final model, variables with a p value of <0.05 were considered significant at 95% confidence interval and the strength of association was measured using odds ratio. Results. Primary education (AOR = 0.104, 95% CI (0.02–0.48)), secondary education (AOR = 0.48, 95% CI (0.24–0.952)), women who have no child (AOR = 2.04, 95% CI (1.2–3.4)), women who had no discussion with their partner (AOR = 2.2, 95% CI (1.39–3.57)), mass counseling (AOR = 3.5, 95% CI (1.75–7.01)), women who had no counseling about side effects (AOR = 1.7, 95% CI (1.07–2.07)), women who experienced side effects (AOR = 2.2, 95% CI (1.4–3.4)), and purpose of family planning use (AOR = 2.5, 95% CI (1.14–4.8)) were determinants of Implanon discontinuation. Conclusion. Implanon discontinuation is attributed by multifactorial involvement. Women’s educational status, nulliparity, no counseling, not informed of side effects, and no partner discussion are significant factors. Health sector stakeholders need to tailor counseling services at individual level to bolster family planning utilization until the desired time.
Association between Physical Activity and Age among Children with Overweight and Obesity: Evidence from the 2016‐2017 National Survey of Children’s Health
Authors: Li Xun , Xiang Shi-Ting , Dong Jie , Zhong Yan , Zhao Sha , Xiao Zhenghui , Li Liping
Published: September 2020

Physical activity participation in children declines with age. It is not clear yet whether the age‐related trends vary by weight status. This study is aimed at investigating the association between physical activity participation and age among children with healthy weight, overweight, or obesity, using data from the 2016‐2017 National Survey of Children’s Health (NSCH). Physical activity participation was evaluated by days participated in physical activity for at least 60 minutes out of 7 days. Weight status was categorized from body mass index (BMI) percentiles. Data were analyzed on 33,056 US children age 10‐17 years. The percentages of been active 0 day out of 7 days in BMI5th < 85th (healthy weight), 85th < 95th (overweight), and ≥95th percentile (obese) groups were 8.9%, 11.5%, and 18.2%, respectively. Among all groups, been active 0 day out of 7 days was positively associated with age, while the strongest associations were observed in the BMI85th < 95th group (age 17 years vs. age 10 years: OR = 7.48, p < 0.0001). Older age was significantly associated with been active less than 4 days out of 7 days in the BMI5th < 85th and 85th < 95th groups, but those associations were attenuated in the BMI ≥ 95th group. This study found that physical activity participation was inversely associated with age among children with healthy weight, overweight, or obese, and the association was strongest among children with overweight and weakest among children with obesity. Interventions aimed at promoting physical activity among children should take these patterns of association into account.
Characterizing CDK12-Mutated Prostate Cancers
Authors: Rescigno Pasquale , Gurel Bora , Pereira Rita , Crespo Mateus , Rekowski Jan , Rediti Mattia , Barrero Maialen , Mateo Joaquin , Bianchini Diletta , Messina Carlo , Fenor de la Maza Maria D. , Chandran Khobe , Carmichael Juliet , Guo Christina , Paschalis Alec , Sharp Adam , Seed George , Figueiredo Ines , Lambros Maryou , Miranda Susana , Ferreira Ana , Bertan Claudia , Riisnaes Ruth , Porta Nuria , Yuan Wei , Carreira Suzanne , de Bono Johann S.
Published: September 2020

Purpose:

Cyclin-dependent kinase 12 (CDK12) aberrations have been reported as a biomarker of response to immunotherapy for metastatic castration-resistant prostate cancer (mCRPC). Herein, we characterize CDK12-mutated mCRPC, presenting clinical, genomic, and tumor-infiltrating lymphocyte (TIL) data.

Experimental Design:

Patients with mCRPC consented to the molecular analyses of diagnostic and mCRPC biopsies. Genomic analyses involved targeted next-generation (MiSeq; Illumina) and exome sequencing (NovaSeq; Illumina). TILs were assessed by validated immunocytochemistry coupled with deep learning–based artificial intelligence analyses including multiplex immunofluorescence assays for CD4, CD8, and FOXP3 evaluating TIL subsets. The control group comprised a randomly selected mCRPC cohort with sequencing and clinical data available.

Results:

Biopsies from 913 patients underwent targeted sequencing between February 2015 and October 2019. Forty-three patients (4.7%) had tumors with CDK12 alterations. CDK12-altered cancers had distinctive features, with some revealing high chromosomal break numbers in exome sequencing. Biallelic CDK12-aberrant mCRPCs had shorter overall survival from diagnosis than controls [5.1 years (95% confidence interval (CI), 4.0–7.9) vs. 6.4 years (95% CI, 5.7–7.8); hazard ratio (HR), 1.65 (95% CI, 1.07–2.53); P = 0.02]. Median intratumoral CD3+ cell density was higher in CDK12 cancers, although this was not statistically significant (203.7 vs. 86.7 cells/mm2; P = 0.07). This infiltrate primarily comprised of CD4+FOXP3− cells (50.5 vs. 6.2 cells/mm2; P < 0.0001), where high counts tended to be associated with worse survival from diagnosis (HR, 1.64; 95% CI, 0.95–2.84; P = 0.077) in the overall population.

Conclusions:

CDK12-altered mCRPCs have worse prognosis, with these tumors surprisingly being primarily enriched for CD4+FOXP3− cells that seem to associate with worse outcome and may be immunosuppressive.

See related commentary by Lotan and Antonarakis, p. 380
Evaluation of Trends and Prognosis Over Time in Patients with AML Relapsing After Allogeneic Hematopoietic Cell Transplant Reveals Improved Survival for Young Patients in Recent Years
Authors: Bazarbachi Ali , Schmid Christoph , Labopin Myriam , Beelen Dietrich , Wolfgang Blau Igor , Potter Victoria , Niittyvuopio Riitta , Socié Gerard , Blaise Didier , Sanz Jaime , Ciceri Fabio , Abou Dalle Iman , Spyridonidis Alexandros , Bug Gesine , Esteve Jordi , Savani Bipin N. , Nagler Arnon , Mohty Mohamad
Published: September 2020

Purpose:

Relapsed acute myeloid leukemia (AML) post allogeneic hematopoietic cell transplantation (allo-HCT) has a dismal prognosis.

Experimental Design:

To assess prognosis of patients with recurrent AML post allo-HCT over time, we analyzed European Society for Blood and Marrow Transplantation registry data of 8,162 adult patients with AML who relapsed between 2000 and 2018 after allo-HCT performed in first complete remission from matched sibling, unrelated, or haploidentical donors.

Results:

The 2-year overall survival (OS) rate from relapse was 17%. For 3,630 patients, <50 years of age, the 2-year OS continuously increased from 16% between 2000 and 2004 to 18% for 2005–2009, to 21% for 2010–2014, and to 26% for 2015–2018 (P = 0.001). Improvement over time was noted both after relapse within and beyond 6 months from allo-HCT. On multivariate analysis among patients <50 years of age, OS was positively affected by a later year of relapse (baseline: 2000–2004; HR, 0.82; P < 0.02 for 2010–2014 and HR, 0.72; P = 0.0002 for 2015–2018), good performance status, favorable cytogenetics, and longer time from transplant to relapse, but negatively affected by increasing age. In contrast, among 4,532 patients, >50 years of age, the year of relapse had no influence on OS (16% for 2000–2004 and 14% for 2015–2018; P = 0.56). Regarding treatment, encouraging results were observed after second allo-HCT, which was performed within 2 years after relapse in 17% of the entire cohort, resulting in a 2-year OS of 30.7%.

Conclusions:

Outcome after posttransplant relapse among younger patients has improved significantly in recent years, likely reflecting, among other factors, the efficacy of posttransplant salvage including second allo-HCT.
Molecular detection and occurrence of equine theileriosis in Arabian horses in Al-Najaf province/Iraq
Authors: Al-Rammahi Hayder Mohammad , Hatem Abdulameer Abed , Al-Atabi Asaad Chasib
Published: September 2020

This study was designed to detect equine piroplasmosis using the molecular technique in Al-Najaf province during the season that showed an increment in tick activities. Blood samples were collected from 110 horses with more than two signs of piroplasmosis. After DNA extraction, the product was examined by a polymerase chain reaction to amplify 18SrRNA. The results showed that the overall percentage of equine theileriosis was 38.18%. According to gender, the percentage of infection was 43.48% and 29.27% in females and males, respectively. Significant variations appeared between infected horses according to age, and the percentage of infection was 50% and 35.22% in less than 2 years and more than 2 years age, respectively. Moreover, the percentage of infection was 62.5% and 19.35% in animals with and without acariasis, respectively. Significant variations were also seen in equine theileriosis according to geographical areas, and the higher percentage was reported in Hera district (60.87%), while the lowest percentage was in the center of Al-Najaf (21.43%). This difference may be due to different distribution of vector of disease (tick), which may be the availability of the suitable weather that helped in the multiplication of the intermediate vectors. In conclusion, this study proved the variations in the occurrences of equine piroplasmosis according to gender, age, and geographical areas.
Adrenal function evaluation in critically ill dogs with low doses of synthetic ACTH
Authors: Jericó Márcia Marques , Bento Fernando Mathias , Silva Ricardo Duarte , Cardozo Felipe Braz de Siqueira , Ponce Fabiano De Granville , Soila Rogério Márcio , Furtado Priscila Viau , Machado Fabrício Lorenzini Aranha
Published: September 2020

The hypothalamus-pituitary-adrenal axis function may be impaired in patients with critical illnesses, especially cases of sepsis, named critical illness-related corticosteroid insufficiency (CIRCI). This study examined the function of the hypothalamic-pituitary-adrenal axis in normal dogs (n = 10) and dogs with critical diseases (n = 16), through determinations of endogenous ACTH (adrenocorticotropic hormone), basal cortisol and cortisol after stimulation in low doses of synthetic ACTH (1.0μg/kg/IV). The stimulation test with ACTH dose tested was verified as effective for evaluation of adrenal function in healthy and sick dogs. Ill dogs differed from healthy dogs by presenting higher basal cortisol values. Eight sick dogs presented a decrease in endogenous ACTH, basal cortisol, or Δ-cortisol. No significant differences were found between the control groups and critically ill dogs for the values of endogenous ACTH, cortisol after stimulation or Δ-cortisol. We concluded that the stimulation test with low-dose ACTH was effective for evaluation of adrenal function, as well as the fact that a considerable portion of critically ill dogs studied here, especially with sepsis, had evidence of inadequate corticosteroid response to stress.
Predicting the Risk of Right Ventricular Failure in Patients Undergoing Left Ventricular Assist Device Implantation
Authors: Frankfurter Claudia , Molinero Micaela , Vishram-Nielsen Julie K.K. , Foroutan Farid , Mak Susanna , Rao Vivek , Billia Filio , Orchanian-Cheff Ani , Alba Ana Carolina
Published: September 2020

Background:

Right ventricular failure (RVF) is a cause of major morbidity and mortality after left ventricular assist device (LVAD) implantation. It is, therefore, integral to identify patients who may benefit from biventricular support early post-LVAD implantation. Our objective was to explore the performance of risk prediction models for RVF in adult patients undergoing LVAD implantation.

Methods:

A systematic search was performed on Medline, Embase, Cochrane Central Register of Controlled Trials, and Cochrane Database of Systematic Reviews from inception until August 2019 for all relevant studies. Performance was assessed by discrimination (via C statistic) and calibration if reported. Study quality was assessed using the Prediction Model Risk of Bias Assessment Tool criteria.

Results:

After reviewing 3878 citations, 25 studies were included, featuring 20 distinctly derived models. Five models were derived from large multicenter cohorts: the European Registry for Patients With Mechanical Circulatory Support, Interagency Registry for Mechanically Assisted Circulatory Support, Kormos, Pittsburgh Bayesian, and Mechanical Circulatory Support Research Network RVF models. Seventeen studies (68%) were conducted in cohorts implanted with continuous-flow LVADs exclusively. The definition of RVF as an outcome was heterogenous among models. Seven derived models (28%) were validated in at least 2 cohorts, reporting limited discrimination (C-statistic range, 0.53–0.65). Calibration was reported in only 3 studies and was variable.

Conclusions:

Existing RVF prediction models exhibit heterogeneous derivation and validation methodologies, varying definitions of RVF, and are mostly derived from single centers. Validation studies of these prediction models demonstrate poor-to-modest discrimination. Newer models are derived in cohorts implanted with continuous-flow LVADs exclusively and exhibit modest discrimination. Derivation of enhanced discriminatory models and their validations in multicenter cohorts is needed.
Microanatomy of the Human Atherosclerotic Plaque by Single-Cell Transcriptomics
Authors: Depuydt Marie A.C. , Prange Koen H.M. , Slenders Lotte , Örd Tiit , Elbersen Danny , Boltjes Arjan , de Jager Saskia C.A. , Asselbergs Folkert W. , de Borst Gert J. , Aavik Einari , Lönnberg Tapio , Lutgens Esther , Glass Christopher K. , den Ruijter Hester M. , Kaikkonen Minna U. , Bot Ilze , Slütter Bram , van der Laan Sander W. , Yla-Herttuala Seppo , Mokry Michal , Kuiper Johan , de Winther Menno P.J. , Pasterkamp Gerard
Published: September 2020

Rationale:

Atherosclerotic lesions are known for their cellular heterogeneity, yet the molecular complexity within the cells of human plaques has not been fully assessed.

Objective:

Using single-cell transcriptomics and chromatin accessibility, we gained a better understanding of the pathophysiology underlying human atherosclerosis.

Methods and Results:

We performed single-cell RNA and single-cell ATAC sequencing on human carotid atherosclerotic plaques to define the cells at play and determine their transcriptomic and epigenomic characteristics. We identified 14 distinct cell populations including endothelial cells, smooth muscle cells, mast cells, B cells, myeloid cells, and T cells and identified multiple cellular activation states and suggested cellular interconversions. Within the endothelial cell population, we defined subsets with angiogenic capacity plus clear signs of endothelial to mesenchymal transition. CD4
+
and CD8
+
T cells showed activation-based subclasses, each with a gradual decline from a cytotoxic to a more quiescent phenotype. Myeloid cells included 2 populations of proinflammatory macrophages showing IL (interleukin) 1B or TNF (tumor necrosis factor) expression as well as a foam cell-like population expressing TREM2 (triggering receptor expressed on myeloid cells 2) and displaying a fibrosis-promoting phenotype. ATACseq data identified specific transcription factors associated with the myeloid subpopulation and T cell cytokine profiles underlying mutual activation between both cell types. Finally, cardiovascular disease susceptibility genes identified using public genome-wide association studies data were particularly enriched in lesional macrophages, endothelial, and smooth muscle cells.

Conclusions:

This study provides a transcriptome-based cellular landscape of human atherosclerotic plaques and highlights cellular plasticity and intercellular communication at the site of disease. This detailed definition of cell communities at play in atherosclerosis will facilitate cell-based mapping of novel interventional targets with direct functional relevance for the treatment of human disease.
Genetic and Environmental Influences on Blood Pressure and Body Mass Index in the National Academy of Sciences–National Research Council World War II Veteran Twin Registry
Authors: Wang Bin , Wu Ting , Neale Michael C. , Verweij Renske , Liu Gaifen , Su Shaoyong , Snieder Harold
Published: September 2020

Blood pressure (BP) and obesity phenotypes may covary due to shared genetic or environmental factors or both. Furthermore, it is possible that the heritability of BP differs according to obesity status—a form of G×E interaction. This hypothesis has never been tested in White twins. The present study included 15 924 White male twin pairs aged between 15 and 33 years from the National Academy of Sciences–National Research Council World War II Veteran Twin Registry. Systolic and diastolic BPs, as well as height and weight, were measured at the induction physical examination. Body mass index (BMI) was used as the index of general obesity. Quantitative genetic modeling was performed using Mx software. Univariate analysis showed that narrow sense heritabilities (95% CI) for systolic BP, diastolic BP, height, and BMI were 0.401 (0.381–0.420), 0.297 (0.280–0.320), 0.866 (0.836–0.897), and 0.639 (0.614–0.664), respectively. Positive phenotypic correlations of BMI with systolic BP (r=0.13) and diastolic BP (r=0.08) were largely due to genetic factors (70% and 86%, respectively). The gene-BMI interaction analysis did not show any support for a modifying effect of BMI on genetic and environmental influences of systolic BP and diastolic BP. Our results suggest that correlations between BP and BMI are mainly explained by common genes influencing both. Higher BMI levels have no influence on the penetrance of genetic vulnerability to elevated BP. These conclusions may prove valuable for gene-finding studies.
From Margin to Institution: Design as a Marketplace for Action in Organizations
Authors: Lee Kipum
Published: September 2020

To resolve the problem of design marginalization in organizations, we introduce the concept of institution and an Aristotelian framework for human activity to identify an action-based approach for design in complex systems. When design is positioned solely as an activity of production—despite the success of human-centered products—it misses an opportunity to influence institutions, or the human-side of organizations. When repositioned as a practice of shaping action, design engages in the ordering, modifying and reordering of social structures, environments and culture. In short, a theory of institution provides a human-centered approach to systems design and organizational transformation.
Technology and More-Than-Human Design
Authors: Giaccardi Elisa , Redström Johan
Published: September 2020

Are we reaching the limits of what human-centered and user-centered design can cope with? Developing new design methodologies and tools to unlock the potentials of data technologies such as the Internet of Things, Machine Learning and Artificial Intelligence for the everyday job of design is necessary but not sufficient. There is now a need to fundamentally question what happens when human-centered design is unable to effectively give form to technology, why this might be the case, and where we could look for alternatives.
Exploring the Nuances of Designing (with/for) Artificial Intelligence
Authors: Stoimenova Niya , Price Rebecca
Published: September 2020

A fundamental shift in the way society operates is approaching driven by advances in the field of artificial intelligence (AI). Yet, there is a comparative lack of discourse across the design discipline regarding this topic. While there are fragments of methodological readiness for designing (with/for) AI, the nuances of such need to be further explored. The aim of this article is to shed light on these and suggest a possible way forward for design that can ensure AI-powered artifacts remain safe even as their utility evolves over time.
The Culture of Ambiguity: Ma in Japanese Culture and Design Movement Posters
Authors: Nguyen Hung Ky
Published: September 2020

In a literal sense, the word ma (間) means “interval” or “gap.” With considerable enthusiasm, since the early 1960s, Japanese thinkers and design practitioners have developed ma into an abstract and spiritual concept. With the use of interpretive research and ethnographic methods, this article explores how ma, commonly understood as ambiguity, has been deeply appreciated in Japanese culture. To find out more about ma, the author interviewed Nagai Kazumasa and Sugiura Kohei, the two eminent Japanese Design Movement (JDM) poster designers, in their Tokyo design offices in 2008. Specifically, from a case study research of one of Sugiura's renowned works, the author found that the concept of ma has been elevated by Sugiura to a religio-aesthetic and spiritual status. Sugiura has used ma as both medium and message in maintaining his intellectual and artistic position in Japan and Asia.
Are the Effects of Financial Market Disruptions Big or Small?
Authors: Barnichon Regis , Matthes Christian , Ziegenbein Alexander
Published: September 2020

While episodes of financial distress are followed by large and persistent drops in economic activity, structural time series analyses point to relatively mild and transitory effects of financial market disruptions. We argue that these seemingly contradictory findings are due to the asymmetric effects of financial shocks, which have been predicted theoretically but not taken into account empirically. We estimate a model designed to identify the (possibly asymmetric) effects of financial market disruptions, and we find that a favorable financial shock—an easing of financial conditions—has little effect on output, but an adverse shock has large and persistent effects. In a counterfactual exercise, we find that over two-thirds of the gap between current US GDP and its 207 precrisis trend was caused by the 2007–2008 financial shocks.
Tax-Preferred Savings Vehicles: Can Financial Education Improve Asset Location Decisions?
Authors: Boyer M. Martin , d'Astous Philippe , Michaud Pierre-Carl
Published: September 2020

In this study, we conduct a stated-choice experiment to analyze the decision to contribute to either a front- or back-loaded tax-preferred retirement savings account. Our experimental design includes a randomized financial education intervention that provides information on the tax implications of both types of account. Respondents who were exposed to the intervention have greater knowledge of these accounts and make contribution choices that increase their after-tax income. Using a well-defined benchmark, we show that on average, respondents who experienced the intervention increase their discounted welfare by about 4% of their contribution amount.
Potential multidimensional behavioural impacts of differential infection in two fish populations
Authors: Ruehle Brandon P. , Poulin Robert
Published: September 2020

Parasites can influence the behaviour of their hosts, however investigations on how they may shape multiple personality traits are uncommon. The flatworm parasite Tylodelphys darbyi resides in the eyes of common bully, Gobiomorphus cotidianus, a locally common fish host to a range of other parasites that could also influence their behaviour. Here we assess how parasitism may be related to personality traits of two G. cotidianus populations; one where T. darbyi is highly abundant and one where the parasite is absent. We hypothesized that T. darbyi would have a stronger association with the different personality traits than other parasite taxa, and that the effects of infection on personality traits would vary between populations. Our results demonstrate that T. darbyi infections correlate with boldness, exploration, and activity within and among individuals. Further, we show that the relationship and therefore possible influence of other parasites, e.g., Apatemon sp., on personality traits vary between two host populations. Our study has revealed potential patterns highlighting how parasitism may differentially contribute to behavioural and ecological divergence among host populations.
The Eophreatoicus Nicholls, 1926 species flock from Kakadu and Arnhem Land, with a description of a new genus of Amphisopidae (Crustacea : Isopoda : Phreatoicidea)
Authors: WILSON GEORGE D.F. , HUMPHREY CHRISTOPHER L.
Published: September 2020

We present descriptions of 28 new species of Amphisopidae from Arnhem Land and Kakadu National Park of Australia’s Northern Territory. We identified five additional species that are not yet fully characterized and are not provided with formal species recognition. This is the first taxonomic treatment highlighting the high species richness within the Phreatoicidea that occur in Australia and likely elsewhere. We document each species fully with scanning electron and light micrographic images, diagnoses, detailed descriptions, keys to identification and justification of each species using parsimony analysis of their morphological and genetic characters. The distributional data show that all species, except for one, have microendemic distributions, with some sibling species occurring within a few kilometers of each other. Because of the age of this group of species, they appear to have spread throughout the region of the Arnhem sandstone plateau and then back-colonized the same habitats so that as many as three morphologically and genetically distinct species may co-occur syntopically. Our research has uncovered a new genus-level taxon of the family Amphisopidae, Kakadubeh gen. nov. This new genus is unlike Eophreatoicus, not only in its general appearance, but also in having an inferred reproductive strategy different from most of the other members of the family. While Eophreatoicus species have males that are much larger than the females and practice precopula, a form of pre-insemination mate guarding, males of the new species, Kakadubeh rangemyahwurd sp. nov., are much smaller than females. In addition, males of this species have a fourth walking leg that is not specialized for holding females, suggesting that they have a reproductive strategy that does not involve precopula of the form seen in Eophreatoicus and Eremisopus Wilson & Keable, 2002a. Most of our research has been undertaken in Kakadu National Park, although recent collections have been made in Arnhem Land, yielding additional distinctive species. Given the size of unexplored territory around the Arnhem Plateau and the geographic frequency of discovering new species, we predict that the diversity of this group in the Northern Territory may be many more than the ~35 species described here. At this time, these microendemic isopods appear to be unthreatened by human activities, largely owing to the environmental protection afforded by Kakadu National Park and Arnhem Land, and their cryptic habits during the dry season. Because they are dependent on small springs of permanent groundwater, future changes in hydrology owing to water use and climate change, as well as invasive introduced species, may present risks to populations and species.