bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigating the antimicrobial and immunomodulatory effects of honeybee venom peptide apamin in theDrosophilagenetic platform
Authors: Wei Yanan , Jia Wenjie , Sun Yanying , Zhang Tianmu , Miao Hongyu , Wu Zekun , Dong Ran , Ning Fangyong , Kim Woo Jae
Published: July 2024

Apamin, an 18-amino acid peptide neurotoxin, constitutes a significant portion of honeybee venom. Although traditionally recognized for its neurotoxic effects, our research demonstrates that apamin exhibits potent antimicrobial properties when genetically expressed inDrosophila. The antimicrobial efficacy of apamin is independent of its disulfide bonds and is enhanced when the peptide is membrane-tethered. This expression selectively targets and inhibits specific harmful bacterial species, such asPseudomonas aeruginosa,Enterococcus faecalis, andEscherichia coli, while promoting beneficial bacteria likeLactobacillus plantarumthereby improving the gut microbiome. The antimicrobial activity of apamin is localized to the gut and is associated with increased proliferation of intestinal stem cells, acidification of the midgut pH, and activation of enteroendocrine cell calcium signaling. Furthermore, apamin’s antimicrobial function is dependent on specific peptidoglycan recognition proteins, with PGRP-LA and PGRP-SCs being essential. Apamin expression alone is sufficient to restore the integrity of the gut barrier compromised by stressed conditions. Ultimately, apamin supplementation enhances honeybee gut health, particularly in the presence of ingested bacteria. The expression of other honeybee antimicrobial peptides also significantly reduces bacterial infection in flies. Overall, our study provides a comprehensive understanding of the molecular function and regulation of honeybee venom peptides and antimicrobial peptides, utilizing theDrosophilamodel system to unravel their mechanisms of action and therapeutic potential.
Modulation of Neuronal Excitability and Plasticity by BHLHE41 Conveys Lithium Non-Responsiveness
Authors: Stephan Marius , Papiol Sergi , Zhang Mingyue , Song Jie , Frommeyer Samuel M. , Haupt Helen , Jensen Niels , Kannaiyan Nirmal , Gupta Rajinder , Schuler Philipp , Picklmann Pia , McCarthy Michael , Schulte Eva , Landen Mikael , Falkai Peter , Scheuss Volker , Schulze Thomas , Zhang Weiqi , Rossner Moritz J.
Published: July 2024

Many bipolar disorder (BD) patients are non-responsive to lithium. The mechanisms underlying lithium (non-)responsiveness are largely unknown. By using gene-set enrichment analysis methods, we found that core clock gene-sets are significantly associated with lithium response. Among the top hits wasBHLHE41, a modulator of the molecular clock and homeostatic sleep. Since BHLHE41 and its paralog BHLHE40 are functionally redundant, we assessed chronic lithium response in double-knockout mutant mice (DKO). We demonstrated that DKOs are non-responsive to lithium’s effect in various behavioral tasks. Cellular assays and patch clamp recordings revealed lowered excitability and reduced lithium-response in prefrontal cortical layer 2/3 DKO neurons and on hippocampal long-term potentiation. Single-cell RNA sequencing identified that lithium deregulated mitochondrial respiration, cation channel and postsynapse associated gene-sets specifically in upper layer excitatory neurons. Our findings show that lithium acts in a highly cell-specific way on neuronal metabolism and excitability and modulates synaptic plasticity depending on BHLHE40/41.
Curved vortex surfaces in four-dimensional superfluids. II. Equal-frequency double rotations
Authors: McCanna Ben , Price Hannah M.
Published: July 2024

As is well known, two-dimensional and three-dimensional superfluids under rotation can support topological excitations such as quantized point vortices and line vortices, respectively. Recently, we have studied how, in a hypothetical four-dimensional (4D) superfluid, such excitations can be generalized to vortex planes and surfaces. In this paper we continue our analysis of skewed and curved vortex surfaces based on the 4D Gross-Pitaevskii equation and show that certain types of such states can be stabilized by equal-frequency double rotations for suitable parameters. This work extends the rich phenomenology of vortex surfaces in four dimensions and raises interesting questions about vortex reconnections and the competition between various vortex structures which have no direct analog in lower dimensions.

Published by the American Physical Society
2024
Observing black hole mergers beyond the pair-instability mass gap with next-generation gravitational wave detectors
Authors: Franciolini Gabriele , Kritos Konstantinos , Reali Luca , Broekgaarden Floor , Berti Emanuele
Published: July 2024

Stellar evolution predicts the existence of a mass gap for black hole remnants produced by pair-instability supernova dynamics, whose lower and upper edges are very uncertain. We study the possibility of constraining the location of the upper end of the pair-instability mass gap, which is believed to appear around mmin∼130M⊙, using gravitational wave observations of compact binary mergers with next-generation ground-based detectors. While high metallicity may not allow for the formation of first-generation black holes on the “far side” beyond the gap, metal-poor environments containing population III stars could lead to such heavy black hole mergers. We show that, even in the presence of contamination from other merger channels, next-generation detectors will measure the location of the upper end of the mass gap with a relative precision close to Δmmin/mmin≃4%(Ndet/100)−1/2 at 90% CL, where Ndet is the number of detected mergers with both members beyond the gap. These future observations could reduce current uncertainties in nuclear and astrophysical processes controlling the location of the gap.

Published by the American Physical Society
2024
Effective mass and symmetry breaking in the Ishibashi-Kawai-Kitazawa-Tsuchiya matrix model from compactification
Authors: Laliberte Samuel
Published: July 2024

The Ishibashi-Kawai-Kitazawa-Tsuchiya (IKKT) model is a promising candidate for a nonperturbative description of type IIB superstring theory. It is known from analytic approaches and numerical simulations that the IKKT matrix model with a mass term admits interesting cosmological solutions. However, this mass term is often introduced by hand and serves as a regulator in the theory. In the present paper, we show that an effective mass matrix can arise naturally in the IKKT model by imposing a toroidal compactification where the space-time fermions acquire antiperiodic boundary conditions. When six spatial dimensions are chosen to be compact, the effective mass matrix breaks the SO(1,9) space-time symmetry of the IKKT model to SO(1,3)×SO(6). This paves the way for space-time solutions of the IKKT model where SO(1,9) symmetry is naturally broken to SO(1,3)×SO(6).

Published by the American Physical Society
2024
Unpacking service failures in artificial intelligence: future research directions
Authors: Chopra Ritika , Bhardwaj Seema , Thaichon Park , Nair Kiran
Published: July 2024

Purpose

The present study undertakes an extensive review of the causes of service failures in artificial intelligence (AI) technology literature.

Design/methodology/approach

A hybrid review has been employed which includes descriptive analysis, and bibliometric analysis with content analysis of the literature approach to synthesizing existing research on a certain topic. The study has followed the SPAR-4-SLR protocol as outlined by Paul et al. (2021). The search period encompasses the progression of service failure in AI from 2001 to 2023.

Findings

From identified theories, theoretical implications are derived, and thematic maps direct future research on topics such as data mining, smart factories, and among others. The key themes are being proposed incorporates technological elements, ethical deliberations, and cooperative endeavours.

Originality/value

This research study makes a valuable contribution to understanding and reducing service defects in AI by providing insights that can inform future investigations and practical implementations. Six key future research directions are derived from the thematic and cluster discussions presented in the content analysis.
Linear stability analysis of micropolar nanofluid flow across the accelerated surface with inclined magnetic field
Authors: Mahabaleshwar U.S. , Sachin S.M. , Vishalakshi A.B. , Bognar Gabriella , Sunden Bengt Ake
Published: July 2024

Purpose

The purpose of this paper is to study the two-dimensional micropolar fluid flow with conjugate heat transfer and mass transpiration. The considered nanofluid has graphene nanoparticles.

Design/methodology/approach

Governing nonlinear partial differential equations are converted to nonlinear ordinary differential equations by similarity transformation. Then, to analyze the flow, the authors derive the dual solutions to the flow problem. Biot number and radiation effect are included in the energy equation. The momentum equation was solved by using boundary conditions, and the temperature equation solved by using hypergeometric series solutions. Nusselt numbers and skin friction coefficients are calculated as functions of the Reynolds number. Further, the problem is governed by other parameters, namely, the magnetic parameter, radiation parameter, Prandtl number and mass transpiration. Graphene nanofluids have shown promising thermal conductivity enhancements due to the high thermal conductivity of graphene and have a wide range of applications affecting the thermal boundary layer and serve as coolants and thermal management systems in electronics or as heat transfer fluids in various industrial processes.

Findings

Results show that increasing the magnetic field decreases the momentum and increases thermal radiation. The heat source/sink parameter increases the thermal boundary layer. Increasing the volume fraction decreases the velocity profile and increases the temperature. Increasing the Eringen parameter increases the momentum of the fluid flow. Applications are found in the extrusion of polymer sheets, films and sheets, the manufacturing of plastic wires, the fabrication of fibers and the growth of crystals, among others. Heat sources/sinks are commonly used in electronic devices to transfer the heat generated by high-power semiconductor devices such as power transistors and optoelectronics such as lasers and light-emitting diodes to a fluid medium, thermal radiation on the fluid flow used in spectroscopy to study the properties of materials and also used in thermal imaging to capture and display the infrared radiation emitted by objects.

Originality/value

Micropolar fluid flow across stretching/shrinking surfaces is examined. Biot number and radiation effects are included in the energy equation. An increase in the volume fraction decreases the momentum boundary layer thickness. Nusselt numbers and skin friction coefficients are presented versus Reynolds numbers. A dual solution is obtained for a shrinking surface.
Accurate targeting in social assistance programs in Southern Madagascar
Authors: Raholiarimanana Fanantenana , Ishida Akira
Published: July 2024

Purpose

 Social assistance programs have been implemented in Madagascar to assist the most vulnerable and neediest poor in achieving a minimum standard of living during critical times. In the Grand South and Southeast Madagascar, where climate change, price fluctuations and sociopolitical instability threaten the population’s livelihood, the effectiveness of such aid-based programs is uncertain because of possible mistargeting. This study aims to assess the accuracy of the targeting methodology of four different types of social protection programs in Southern Madagascar.

Design/methodology/approach

The authors draw evidence from a national representative data set and use multiple techniques that integrate targeting performance and regression-based analyses.

Findings

Results show that cash transfers constitute the only program that effectively reaches the poorest 20% living in drought- and cyclone-affected regions. However, mistargeting is likely to occur in food, seed transfers, and the HIMO public works programs. Social inclusion information related to proximity to urban zones, religious affiliation, and number of long-lasting insecticide-treated nets are significantly associated to all program participation. Nevertheless, a serious under-coverage of the eligible poor is noted for Southern Madagascar.

Originality/value

This study is among the first to evaluate the targeting effectiveness of social programs in Madagascar. It introduces a novel approach to evaluating the poverty rate by utilizing a composite-based wealth score. The authors enhance the classic targeting assessment methodology by incorporating geospatial covariates, categorical, geographical and social network information into an econometric model. The study provides a comprehensive view of the main profile of beneficiaries reached by four social assistance programs in Southern Madagascar.
Implementation of block-chain technology in the European Union energy payments and logistic
Authors: Gholizadeh Younes
Published: July 2024

Purpose

The purpose of this paper that will address in this paper are: how to implement and test a reliable Block-chain-based energy system for the EU energy payments and logistic? What are the main results, impacts and implications of a Block-chain-based energy system for the EU energy payments and logistic?

Design/methodology/approach

The main aim of this study is to propose a Block-chain-based method to offer a secure, reliable and transparent method for energy logistic and payments for European Energy Transactions. To accomplish that aim, the research method of the study follows the design science research approach of smart contracts in the EU Energy logistic and payments. This research approach mainly consists of five sequential steps which are (1) problem and motivation identification, (2) solution objectives definition, (3) design and development and (4) demonstration and evaluation.

Findings

Results of data model demonstrate that the Block-chain-based energy system here can be used for any EU energy payments and logistics. Based on the data results of the model that mentioned above enhance the security and the integrity of the energy payments and logistic by using cryptographic techniques and consensus mechanisms to prevent and detect any tampering or falsification of the data on the Block-chain.

Originality/value

This paper represents a genuine piece of research that contributes to the field by providing new insights and understanding. The findings presented are the result of rigorous analysis and have not been previously published or submitted elsewhere for consideration.
The use of the Internet of Things to increase energy efficiency in manufacturing industries
Authors: Reichardt Anna , Murawski Matthias , Bick Markus
Published: July 2024

Purpose

As one of the most energy-intensive sectors, the manufacturing industry is strongly affected by current economic, ecological and political issues and is increasingly looking to reduce its energy consumption through effective management. One important component of energy management is monitoring, which can be improved by using the Internet of Things (IoT). This study aims to identify the factors behind the adoption of IoT energy monitoring systems in manufacturing industries and IoT’s impact on consumption in this context. Moreover, this study explores what constitutes successful implementation and develops recommendations on how companies can best use the generated energy-saving potential.

Design/methodology/approach

For this purpose, an exploratory, inductive research approach is taken in which five semi-structured interviews with IoT energy-monitoring platform providers located in Germany are conducted and additional documents about these platforms are analysed.

Findings

Based on these interviews and documents, this study defines the relevant factors involved in the increased adoption of IoT energy monitoring systems as desired cost reduction, regulatory requirements and customer expectations. Once implemented, a company’s energy efficiency potential strongly depends on its individual situation; however, the transparency characteristic of IoT supports managers in deriving effective energy-saving measures. Additionally, the used efficiency potential is influenced by the level of data collection and organisational capabilities.

Originality/value

To the best of the authors’ knowledge, as the first study, this research combines findings from the energy management and IoT research streams and places them into an organisational context. The application of the fit-viability model as an overarching framework enhances the theoretical contributions of this study. Moreover, focusing on Germany and its substantial industrial sector enables the gathering of important insights and the analysis of the specific use case of industrial IoT, which yields new findings for practitioners as well.
The impact of board gender diversity on firm performance: does critical mass matter?
Authors: Jayaraman J.D. , Smita R. , Nilakantan Narasinganallur
Published: July 2024

Purpose

The study aims to investigate the impact of board gender diversity (BGD) on firm performance (FP) by testing two hypotheses – the existence of a positive relationship between BGD and FP, and the moderating role of a critical mass of female directors on FP. The study also explores whether the association varies across different industries.

Design/methodology/approach

The authors collect data using Bloomberg and CMIE Prowess, from the Bombay Stock Exchange (BSE) 500 index for the period 2008–2018 and employ a robust statistical methodology (Dynamic Panel Data Model).

Findings

A critical mass of female directors positively moderates and strengthens the relationship between BGD and FP. The study fails to find evidence of a direct association between BGD and FP. The study also finds evidence of industry effects.

Research limitations/implications

Though we use a very robust statistical methodology, any modifications in the methodology or choice of a different methodology are likely to change the results. Moreover, some of the findings are statistically significant at the 10% level.

Practical implications

The findings of our study hold particular significance for emerging economies like India where regulatory initiatives aim to enhance gender diversity within boardrooms.

Originality/value

The study contributes to the critical mass literature by examining the association between a critical mass of female directors as a moderating variable of BGD and FP. Further, the study also identifies those industries which show a positive association between FP and BGD.
Playlists and genre: the role of music genre in Spotify’s playlists
Authors: McDonald Callum , Foster Allen Edward , Rafferty Pauline
Published: July 2024

Purpose

Genre is a valuable access point for popular music collections; however, the blurring of genre boundaries combined with changing listening habits and new forms of classification have brought genre’s importance into question. The playlist is now a common means of classification on music streaming platforms. Recent commentary suggests that context is now a preferred access point. This exploratory study offers an examination of genres’ role in playlists.

Design/methodology/approach

A mixed-methods study investigates, using Spotify, whether genre retains relevance amidst the rise in popularity of playlist-based music classification. Sample size is noted as a limitation of the study.

Findings

Qualitative coding of user and editorial playlist names revealed less than 20% of codes applied were genre-based. However, when non-genre themes were differentiated, genre themes ranked as one of the most prevalent. Context-based themes were most common, though genre was readily combined with other descriptive themes, highlighting its utility. Quantitative analysis of genre tags showed playlists with context-based themes demonstrated higher genre homogeneity than those using generic themes, indicating playlists were named on a genre-by-proxy basis.

Originality/value

The study suggests that genre continues to play an integral role in a field where an eclectic variety of descriptive themes has emerged, although its role may have changed. Context-based themes are central to the way users organise music, though such terms can often serve as containers for music collections sharing distinct generic and musicological similarities.
Can supervisor reminders help prevent fraud in the mutual funds sector
Authors: Al-Zyoud Hussein , Wang Eric Zengxiang , Ali Shahid , Liu Weiming
Published: July 2024

Purpose

This study is based on the enforcement record from Canada’s natural mutual fund regulator. This record documented a small subset of mutual fund dealers who had been disciplined for their misconduct from 2007 to 2014. The purpose of this paper is to determine what factors contribute to mutual fund dealers’ time to first financial fraud offense. The longer the time to fraud, the healthier the mutual fund industry and the better a mutual fund dealer’s career.

Design/methodology/approach

Based on the belief that adversity reveals true character, the study approaches a mutual dealer’s career success from human capital, socio-demographic and organizational sponsorship points of view by measuring dealers’ success as their time from career beginning to first instance of financial fraud. Ordinary least square regression analysis was used to identify if those factors, including provision of supervisor reminders, gender, position and penalties, are related to career success within the Canadian mutual fund regulatory framework. The research is based on a small sample of mutual fund dealers who had been disciplined for their misconduct from 2007 to 2014.

Findings

The study finds that a supervisor’s reminders positively contribute to the career success of a mutual fund dealer in the form of extending their time to fraud. As well, being female is an adverse factor to career success even when both female and male dealers received about the same level of supervisor reminders. It also finds that being in a management position has no association with time to fraud.

Originality/value

The study establishes the statistically significant positive relationship between time to fraud and supervisor’s reminders for mutual fund dealers. At the same time, it shows that human capital and access to organizational resources, measured by being in a management position, have no significant relation to when fraud is committed. This result indicates the value of continuing education for all mutual fund dealers, both inexperienced and experienced.
Limitation on payment by cash to combat money laundering: a comparative assessment of the laws of Mauritius and France
Authors: Mahadew Bhavna
Published: July 2024

Purpose

The purpose of this article is to review the legislation on limitation on cash payment from a comparative perspective with France. This legal provision has been the subject of inconsistency and lack of clarity for the past two decades.

Design/methodology/approach

This paper uses a doctrinal legal research technique based on an analysis of the law and how it is interpreted in relation to Mauritius's prohibition on cash payments. Section 5 of the FIAMLA 2002 is interpreted in large part by this assessment using legal precedents from the Supreme Court and other Mauritius judicial authorities. France has been chosen as the comparative jurisdiction as it has well-defined legal framework on cash payment limitations in Europe.

Findings

The Mauritian judiciary has played a far more significant and active role than the French judiciary. A cursory search reveals a dearth of case law concerning the interpretation of the pertinent sections of the French Monetary and Financial Code concerning the prohibition on cash payments. The French legal system places more emphasis on the kinds of transactions that are exempt, whereas the Mauritius system places more attention on the entities that are exempt, such as banks and other financial organisations.

Originality/value

This paper fills in the gap that exists on legal interpretation of legislation on limitation on payment by cash in Mauritius. It originally contributes to legal literature on money laundering by undertaking a comparison between Mauritius and France for the benefits of students and researchers around the world.
Conceptualising place branding in three approaches: towards a new definition of place brands as embodied experiences
Authors: Eshuis Jasper , Ripoll González Laura
Published: July 2024

Purpose

This paper aims to provide conceptual clarity on the different approaches of place branding in the literature. It discusses three main approaches and provides a new definition of place brands that acknowledges the full multi-sensory experience of place brands. This paper also elaborates brand management within the three approaches.

Design/methodology/approach

Conceptual paper

Findings

This study identifies three co-existing approaches of place branding and provides a definition of place brands for each of them. The first approach conceptualises place brands as symbolic constructs that identify and differentiate places from others. Brand symbols such as logos and slogans are central, assuming that brand meaning resides in them. The second approach views place brands as images and associations in the minds of target groups, whereby brands reside in individuals’ minds (the cognitive). This paper aligns with a third approach that views place brands as experiential, multi-sensory constructs. Brands invite not only mental representations in people’s minds but especially also multi-sensory embodied experiences. The authors thus define place brands as marketing systems that consist of dynamic performative assemblages of symbolic, discursive, institutional and material elements that selectively invite certain multi-sensory and embodied experiences of place by stakeholders and target groups.

Originality/value

This paper contributes to conceptual clarity by providing an analytical framework identifying three main approaches to place branding. The authors further reflect on the implications of each approach for brand management. This paper also builds on recent literatures to provide a new and contemporary definition of place brands as multi-sensory experiences that encompasses embodiment.
“Unintended” marketing through influencer vlogs: impacts of interactions, parasocial relationships and perceived influencer credibility on purchase behaviors
Authors: Zhang Ruonan , Mercado Trinideé , Bi Nicky Chang
Published: July 2024

Purpose

Influencers’ vlogs have the potential to impact consumer behaviors through vlog-embedded corporate sponsorship and brand collaborations. However, even without brand involvement, vlogs can also “unintentionally” benefit influencers as a relationship-building tool. This study is designed to investigate the relationship between vlog-viewing and audiences’ purchase behaviors of influencer-recommended products through the impacts of influencer–follower interactions, perceived influencer credibility and parasocial relationships.

Design/methodology/approach

An influencer-disseminated online survey was conducted in collaboration with a YouTube celebrity among N = 948 of her 72.6 K subscribers. Statistical analysis was performed through structural equation modeling (SEM) on SPSS Amos.

Findings

SEM results indicated that the extent to which participants liked the vlogs had both a direct impact on their purchase behaviors and secondary impact through social media engagement, parasocial relationships and perceived influencer credibility.

Originality/value

The study expands current research and understanding of influencer marketing. Brands and social media content creators are advised to rethink vlogs as a creative genre for long-term brand–influencer collaborations and implicit social media endorsements.
Formal financial institutions’ financing of women-owned small and medium enterprises in South Africa: a supply-side perspective
Authors: Asah Francis Tangwo , Hove-Sibanda Progress
Published: July 2024

Purpose

Although women-owned small and medium enterprises (SMEs) represent only 21.1% of all SMEs in South Africa, they play a fundamental role in the SME sector in terms of job creation, employment and poverty alleviation that is critical for economic growth. This study aims to explore (FFIs) financing of women-owned SMEs in South Africa from a credit provider perspective (supply-side).

Design/methodology/approach

A qualitative research approach positioned in the interpretivistic research paradigm was used to accomplish this study objectives. The five-step process of content analysis proposed by Terre Blanche, Durrheim and Kelly was used to analyse the qualitative data collected from the 16 participants via semi-structured in-depth interviews.

Findings

The findings reveal that FFIs are willing to finance women-owned businesses provided they can contribute a reasonable percentage of the equity capital and a first-class collateral. Lack of equity, business experiences and first-class collateral are the most serious challenges faced by FFIs when considering lending to women-owned SMEs.

Originality/value

This study investigated the financing of women-owned SMEs in South Africa from a supply-side perspective, compared to other studies that used quantitative methodology. This study findings provide insights into how FFIs perceive financing women-owned SMEs, women-owned SMEs credit approval rate, the factors that influence the willingness of FFIs to provide credit to women-owned SMEs and the challenges experienced by FFIs in financing women-owned SMEs.
Previous assessments of faecal glucocorticoid metabolites in Cape mountain zebra (Equus zebra zebra) were flawed
Authors: Britnell Jake A. , Palme Rupert , Kerley Graham I. H. , Jackson John , Shultz Susanne
Published: July 2024

Steroid hormones, especially glucocorticoids (GCs), are widely used to assess physiological responses to stressors. As steroid hormones are heavily metabolised prior to excretion, it is essential to validate enzyme immunoassays (EIAs) for measuring faecal glucocorticoid metabolites (fGCMs). Although problems with unvalidated assays have been raised repeatedly, their use persists widely.

Lea et al. (2017) used an unvalidated corticosterone assay (CJM006) to relate fGCM concentrations to habitat quality, demography and population performance in the Cape mountain zebra (Equus zebra zebra). Here, we revisit their findings and evaluate the validity of their conclusions using a validated EIA. First, we evaluate the biological sensitivity of six EIAs (three group‐specific metabolite assays and three corticosterone assays, including CJM006) through a biological validation experiment (translocation) for two sub‐species of mountain zebra, Cape mountain and Hartmann's mountain zebra (E. z. hartmannae). Second, we reanalyse the faecal extracts from Lea et al. (2017) using a validated EIA.

fGCM concentrations consistently increased following translocation, when using two 11‐oxoaetiocholanolone (lab codes: 72T and 72a) and an 11ß‐hydroxyaetiocholanolone (69a) EIA, but did not with three different corticosterone EIAs. All corticosterone EIAs (including CJM006) failed to detect an increase in fGCMs within the critical 48–72‐h period post translocation. Therefore, the CJM006 EIA utilised in Lea et al. (2017) does not sensitively measure hypothalamic–pituitary–adrenal (HPA) axis activity in CMZ faeces.

Using a validated assay (72T), fGCM concentrations were no longer associated with adult sex ratio or habitat quality (measured by grassiness) and these variables were dropped from predictive models. fGCM concentrations now varied between seasons and were negatively associated with female fecundity (foal:mare ratio). Consequently, we can conclude that the results of the previous study are unreliable.

We introduce the terms “insensitive” and “sub‐optimal” to categorise assays that are tested but fail validation, and assays that are comparatively poor at detecting relevant hormone changes, respectively. We discuss how both “insensitive” and “sub‐optimal” assays could lead to incorrect inferences about population stressors and counterproductive conservation recommendations.

Read the free Plain Language Summary for this article on the Journal blog.
Preparation, characterisation and functional effects of goat milk whey protein isolate with low lactose after heat and non‐heat treatments
Authors: Shu Qin , Zhu Li , Li Zekun , Al‐Wraikat Majida , Li Linqiang , Liu Yongfeng
Published: July 2024

This study investigates the physicochemical and functional properties of goat milk whey with heat and non‐heat treatments (pasteurisation, high‐temperature heating, ultrasonic treatment and microwave heating) and lactose hydrolysis. The optimal lactose hydrolysis conditions were obtained through orthogonal test. The results revealed the lactose and glucose in low‐lactose goat milk whey remained largely unchanged through all four treatments, while high‐temperature and microwave treatments induced protein structure alteration. The foaming and emulsifying properties of low‐lactose whey powder were enhanced by heating and ultrasonic treatments, although foam stability decreased. Our findings provide valuable insights into exploiting more nutritional goat milk products.
Assessment of elements (toxic and essential) concentrations and methicillin resistance in Staphylococcus aureus strains isolated from raw milk in the Kurdistan province
Authors: Nassiri Kimia , Raeeszadeh Mahdieh , Ahmadi Elham
Published: July 2024

The presence of residual antibiotics and the excessive accumulation of metals beyond permissible limits in milk represent significant health challenges. This study aimed to analyse the levels of heavy metals and trace elements in raw milk from Kurdistan province using inductively coupled plasma mass spectrometry (ICP‐MS) following digestion via thermal block. Subsequently, the samples were evaluated for contamination with MRSA (methicillin‐resistant Staphylococcus aureus), taking into account the potential correlation between methicillin‐resistant strains and metal accumulation. Finally, milk safety was assessed based on the presence of toxic metals. A total of 180 milk samples were examined. Among them, 13.34% were found to be contaminated with S. aureus, and of those, 62.48% tested positive for MRSA. The metal concentrations (mg/kg) were measured as follows: arsenic 7 × 10−2 ± 6 × 10−3, cadmium 4.70 × 10−1 ± 9.70 × 10−2, lead 7 × 10−2 ± 5 × 10−3, mercury 3 × 10−2 ± 9 × 10−3 and iron 4 × 10−2 ± 9 × 10−3. Interestingly, arsenic, cadmium and iron levels were found to be correlated with the presence of MRSA. Additionally, there was an inverse relationship between toxic metals and essential ones in samples sensitive to methicillin (P < 0.001). The Target Hazard Quotient (THQ) for cadmium is indicated at 9.94. Furthermore, the calculated Carcinogenic Risk (CR) parameters for cadmium and arsenic were 6.06 × 10−3 and 2.22 × 10−4, respectively, suggesting that their concentrations fall within the carcinogenic range. The coexistence of MRSA and metal contamination in raw milk highlights the potential risks linked to its consumption. Consequently, the adoption of rigorous hygiene protocols is imperative to effectively address these concerns.
Therapeutic plasma exchange in autoimmune neurological disorders: A comprehensive evaluation of clinical outcomes, safety, and long‐term disability using the modified Rankin scale
Authors: Bava Davood , Panda Samhita , Jain Romesh , Mittal Siddharth , Gupta Anubhav , Khan Atik , Mandal Saptarshi , Bajpayee Archana
Published: July 2024

Introduction

Autoimmune neurological diseases (ANDs) involve the immune system attacking the nervous system, leading to various symptoms. Therapeutic plasma exchange (TPE) is used to remove pathogenic autoantibodies, aiming to improve clinical outcomes.

Methods

This ambispective observational study included 99 patients with ANDs who underwent TPE from January 2018 to June 2022 at a tertiary care center in India. Clinical outcomes were measured using the modified Rankin Scale (mRS) scores at admission, post‐TPE, at 3‐months, 6‐months, and 1‐year follow‐up post‐discharge. Data were analyzed using Epi Info version 7.0.

Results

The median mRS score improved significantly from 5 (IQR 4–5) before TPE to 3 (IQR 2–4) post‐TPE (p < 0.001). Complications occurred in 5.95% of procedures, with allergic reactions being the most common. The in‐hospital mortality rate was 9%.

Conclusion

TPE is a safe and effective treatment modality for autoimmune neurological diseases, especially in resource‐constrained settings. It aids in both symptomatic relief and reducing long‐term functional disability.
Seed size and dispersal mode select mast seeding in perennial plants
Authors: YU Fei , ZHANG Mingming , YANG Yueqin , WANG Yang , YI Xianfeng
Published: July 2024

Reproduction by perennial plants varies from being relatively constant over years to the production of massive and synchronous seed crops at irregular intervals, a reproductive strategy called mast seeding. The sources of interspecific differences in the extent of interannual variation in seed production are largely unknown. We conducted a global meta‐analysis of animal‐dispersed species to quantify how the interannual variability in seed crops produced by plants can be explained by the seed mass, dispersal mode, phylogeny, and climate. Phylogenetic analysis indicated that the interannual variations in seed production and seed mass tended to be similar in related species due to their shared evolution. The interannual variation in seed production was 1.22 times higher in synzoochorous species dispersed by scatter‐hoarders compared with endozoochorous species dispersed by frugivores. Furthermore, the production of small seeds was associated with higher interannual variation in seed production, although synzoochorous species produced larger seeds than endozoochorous species. Precipitation rather than temperature had a significant positive effect on the interannual variation in seed production. The seed mass and dispersal mode contributed more to the interannual variation in seed production than phylogeny, climate, and fruit type. Our findings support a long‐standing hypothesis that interspecific variation in the masting intensity is largely shaped by interactions between plants and animals.
Microbial drinking water monitoring now and in the future
Authors: Pluym Thomas , Waegenaar Fien , De Gusseme Bart , Boon Nico
Published: July 2024

Over time, humanity has addressed microbial water contamination in various ways. Historically, individuals resorted to producing beer to combat the issue. Fast forward to the 19th century, and we witnessed a scientific approach by Robert Koch. His groundbreaking gelatine plating method aimed to identify and quantify bacteria, with a proposed limit of 100 colony‐forming units per millilitre (CFU/mL) to avoid Cholera outbreaks. Despite considerable advancements in plating techniques through experimentation with media compositions and growth temperatures, the reliance on a century‐old method for water safety remains the state‐of‐the‐art. Even though most countries succeed in producing qualitative water at the end of the production centres, it is difficult to control, and guarantee, the same quality during distribution. Rather than focusing solely on specific sampling points, we propose a holistic examination of the entire water network to ensure comprehensive safety. Current practices leave room for uncertainties, especially given the low concentrations of pathogens. Innovative methods like flow cytometry and flow cytometric fingerprinting offer the ability to detect changes in the microbiome of drinking water. Additionally, molecular techniques and emerging sequencing technologies, such as third‐generation sequencing (MinION), mark a significant leap forward, enhancing detection limits and emphasizing the identification of unwanted genes rather than the unwanted bacteria/microorganisms itself. Over the last decades, there has been the realization that the drinking water distribution networks are complex ecosystems that, beside bacteria, comprise of viruses, protozoans and even isopods. Sequencing techniques to find eukaryotic DNA are necessary to monitor the entire microbiome of the drinking water distribution network. Or will artificial intelligence, big data and machine learning prove to be the way to go for (microbial) drinking water monitoring? In essence, it is time to transcend century‐old practices and embrace modern technologies to ensure the safety of our drinking water from production to consumption.
Unlocking Antarctic molecular time‐capsules – Recovering historical environmental DNA from museum‐preserved sponges
Authors: Jeunen Gert‐Jan , Mills Sadie , Lamare Miles , Duffy Grant A. , Knapp Michael , Stanton Jo‐Ann L. , Mariani Stefano , Treece Jackson , Ferreira Sara , Durán‐Vinet Benjamín , Zavodna Monika , Gemmell Neil J.
Published: July 2024

Marine sponges have recently emerged as efficient natural environmental DNA (eDNA) samplers. The ability of sponges to accumulate eDNA provides an exciting opportunity to reconstruct contemporary communities and ecosystems with high temporal and spatial precision. However, the use of historical eDNA, trapped within the vast number of specimens stored in scientific collections, opens up the opportunity to begin to reconstruct the communities and ecosystems of the past. Here, we define the term ‘heDNA’ to denote the historical environmental DNA that can be obtained from the recent past with high spatial and temporal accuracy. Using a variety of Antarctic sponge specimens stored in an extensive marine invertebrate collection, we were able to recover information on Antarctic fish biodiversity from specimens up to 20 years old. We successfully recovered 64 fish heDNA signals from 27 sponge specimens. Alpha diversity measures did not differ among preservation methods, but sponges stored frozen had a significantly different fish community composition compared to those stored dry or in ethanol. Our results show that we were consistently and reliably able to extract the heDNA trapped within marine sponge specimens, thereby enabling the reconstruction and investigation of communities and ecosystems of the recent past with a spatial and temporal resolution previously unattainable. Future research into heDNA extraction from other preservation methods, as well as the impact of specimen age and collection method, will strengthen and expand the opportunities for this novel resource to access new knowledge on ecological change during the last century.
Challenge of concomitant thymoma resection and myocardial revascularization: A Case Report
Authors: Leonardi Beatrice , Natale Giovanni , Vicario Giuseppe , Grande Mario , Fiorelli Alfonso , Di Tommaso Luigi , Speranza Vincenzo , Torella Daniele , De Feo Marisa , Torella Michele
Published: July 2024

Myocardial revascularization in patients presenting with an anterior mediastinal mass poses considerable challenges. In this report, we outline two cases involving patients with anterior mediastinal masses who underwent surgical resection alongside concurrent myocardial revascularization. One patient underwent coronary artery bypass graft surgery, while the other was treated by percutaneous coronary intervention with drug‐eluting stent placement. Both patients fully recovered from the relative procedures and were discharged within two weeks post‐surgery, ultimately diagnosed with thymoma. The concomitant intervention offered the advantage of promptly addressing both conditions, and it was performed safely through a collaborative multidisciplinary effort.
Pathophysiological processes underlying hidden hearing loss revealed in Kcnt1/2 double knockout mice
Authors: Schubert Nick M. A. , Reijntjes Daniël O. J. , van Tuinen Marcel , Vijayakumar Sarath , Jones Timothy A. , Jones Sherri M. , Pyott Sonja J.
Published: July 2024

Presbycusis is a prevalent condition in older adults characterized by the progressive loss of hearing due to age‐related changes in the cochlea, the auditory portion of the inner ear. Many adults also struggle with understanding speech in noise despite having normal auditory thresholds, a condition termed “hidden” hearing loss because it evades standard audiological assessments. Examination of animal models and postmortem human tissue suggests that hidden hearing loss is also associated with age‐related changes in the cochlea and may, therefore, precede overt age‐related hearing loss. Nevertheless, the pathological mechanisms underlying hidden hearing loss are not understood, which hinders the development of diagnostic biomarkers and effective treatments for age‐related hearing loss. To fill these gaps in knowledge, we leveraged a combination of tools, including transcriptomic profiling and morphological and functional assessments, to identify these processes and examine the transition from hidden to overt hearing loss. As a novel approach, we took advantage of a recently characterized model of hidden hearing loss: Kcnt1/2 double knockout mice. Using this model, we find that even before observable morphological pathology, hidden hearing loss is associated with significant alteration in several processes, notably proteostasis, in the cochlear sensorineural structures, and increased susceptibility to overt hearing loss in response to noise exposure and aging. Our findings provide the first insight into the pathophysiology associated with the earliest and, therefore, most treatable stages of hearing loss and provide critical insight directing future investigation of pharmaceutical strategies to slow and possibly prevent overt age‐related hearing loss.
Effective alleviation of depressive and anxious symptoms and sleep disorders in benzodiazepine‐dependent patients through repetitive transcranial magnetic stimulation
Authors: Chen Jinbo , Chen Zixuan , Zhang Yanli , Fan Xiaohe , Zhang Changchun , Zhu Jun , Song Chuanfu , Zhang Shuangli , Zhang Danwei , Tang Lijuan , Li Benhan , Yang Weibian , Hu Qiang
Published: July 2024

Benzodiazepine (BZD) dependence poses a significant challenge in mental health, prompting the exploration of treatments like repetitive transcranial magnetic stimulation (rTMS). This research aims to assess the impact of rTMS on alleviating symptoms of BZD dependence. A randomized control trial was employed to study 40 BZD‐dependent inpatients. Their symptoms were quantified using the Hamilton Anxiety Rating Scale (HAMA), Montgomery–Åsberg Depression Rating Scale (MADRS) and Pittsburgh Sleep Quality Index (PSQI). Participants were divided into a conventional treatment group (daily diazepam with gradual tapering) with supportive psychotherapy and another group receiving the same treatment supplemented with rTMS (five weekly sessions for 2 weeks). Significant improvements were observed in both groups over baseline in MADRS, HAMA and PSQI scores at the 2nd, 4th, 8th and 12th week assessments (p < 0.05). The group receiving rTMS in addition to conventional treatment exhibited superior improvements in all measures at the 8th and 12th weeks. The addition of rTMS to conventional treatment methods for BZD dependence significantly betters the recovery in terms of depression, anxiety and sleep quality, highlighting the role of rTMS as an effective adjunct therapy.