bluuebunny/crossref_metadata_2025 huggingface dataset:
The effect of cellular nuclear function alteration on the pathogenesis of shoulder adhesive capsulitis. An immunohistochemical study on Lamin A/C expression
Authors: Candela Vittorio , Peruzzi Barbara , Leopizzi Martina , Porta Natale , Maio Valeria Di , Rocca Carlo Della , Gumina Stefano
Published: September 2022

Background: The network of intermediate filament proteins underlying the inner nuclear membrane forms the nuclear lamina. A and B-type lamins are the major components of the nuclear lamina. Lamins have been associated with important cellular functions: DNA replication, chromatin organization, differentiation of the cell, apoptosis and in maintenance of nuclear structure.
Little is known regarding the etiopathogenesis of adhesive capsulitis (AC); recently, a dysregulating fibrotic response starting from a subpopulation has been described within the fibroblast compartment that suddenly turns on an activated phenotype.
Considering the key role of A-type lamin in the regulation of cellular stability and function we performed an immunohistochemical analysis to compare the lamin A/C expression between patients with AC and healthy controls.
Materials and methods: Twenty-six patients [12M-14F, mean age (SD): 52.3 (6.08)] were enrolled. Tissue samples excised from the rotator interval were analysed for lamin A/C expression by immunohistochemistry. Controls were represented by samples obtained by normal rotator interval excised from patients submitted to shoulder surgery [6M-9F, mean age (SD): 57.1 (5.3)]. The intensity of staining was graded, and an H-score was assigned. Statistical analysis was performed.
Results: The expression of lamin A/C was found to be significantly lower in the fibroblasts of patients with adhesive capsulitis when compared to controls (intensity of staining: p: 0.005; H-score: 0.034); no differences were found regarding the synoviocytes (p>0.05). Considering only patients with AC, lamin A/C intensity staining was found to be significantly higher in samples where acute inflammatory infiltrate was detected (p: 0.004).
No significant changes in levels of lamin A/C expression were documented between the mild and severe adhesive capsulitis severity groups.
Conclusions: Our study demonstrated that the activity of lamin A/C in maintaining nuclear structural integrity and cell viability is decreased in patients with adhesive capsulitis. The timing of the pathogenetic process is the key factor for cell functionality which is maintained in the early stages of the disease; on the contrary, the clinical severity of adhesive capsulitis plays a marginal role on nuclear stability.
Level of Evidence: III
Oral Nicotinamide Mononucleotide (NMN) to treat chronic insomnia: Protocol for the multicenter, randomized, double-blinded, placebo-controlled trial.
Authors: Gao Xiangyang , Zeng Qiang , Li Junhua , Xu Sanping , Li Xueying , Wang Xicheng , Li Yongli , Huang Yan , Liu Shaohui
Published: September 2022

Background: The treatment of insomnia, which is the most common sleep disorder, includes drug and behavioral treatment, but each treatment measure has its limitations. So new treatment method needs to be taken to improve the treatment effect. MN supplementation is a potential promising new method for the treatment of insomnia, resulting in a rising need for methodological research towards verifying its efficacy.
Methods/Design: We describe a proposal for a multicenter, patient-assessor-blinded, randomized controlled trial with two parallel arms. A total of 400 chronic insomnia patients will be allocated 1:1 to the intervention group (treatment with oral NMN 320mg/day) or control group (treatment with oral placebo). All subjects are clinical chronic insomnia patients who meet all inclusion criteria. All subjects are treated by taking NMN or placebo.
The primary outcome is the score on the Pittsburgh Sleep Quality Index (PSQI). Secondary outcomes are the score on the Insomnia Severity Index (ISI) and Epworth Sleeping Scale (ESS)，the Total sleep time (TST), sleep efficiency (SE), sleep latency, REM sleep latency to assess sleep quality changes.
Subjects are assessed at two time points: baseline and follow-up. The duration of the clinical trial is 60 days.
Discussion: This study will provide more evidence on the effects of NMNon improving sleep quality among patients with chronic insomnia. If proven effective, NMN supplementcan be used as a new treatment for chronic insomniain the future.
Trial registration:
Chinese Clinical Trial Registry (chictr.org.cn): ChiCTR2200058001. Registered on 26 March 2022.
Ubiquitous Spin Freezing in the Superconducting State of UTe2
Authors: Sonier Jeff , Sundar Shyam , Gheidi Shayan , Azari Nasrin , Goeks Mariah , Abedi Mae , Yakovlev Michael , Blundell Stephen , Wilkinson John , Dunsiger Sarah , Rosa Priscila , Thomas Sean , Movshovich Roman , Lee Sangyun , Woods Andrew , Paglione Johnpierre , Saha Shanta , Butch Nicholas , Metz Tristin , Hayes Iain
Published: September 2022

In most superconductors electrons form Cooper pairs in a spin-singlet state mediated by either a quantum of energy from a crystal lattice vibration (phonon) or by long-range interactions such as spin fluctuations. The novel superconductor UTe2 is a rare material wherein electrons form pairs in a unique spin-triplet state with potential topological properties. Theoretically, spin-triplet superconductivity in UTe2 may be explained in terms of pairing mediated by either ferromagnetic or antiferromagnetic fluctuations. Experimentally, the magnetic properties of UTe2 remain enigmatic. Here we report muon spin rotation/relaxation (μSR) measurements on independently grown UTe2 single crystals that exhibit either a single or double phase transition in the specific heat near the onset of superconductivity. In the absence of an applied magnetic field, we observe an inhomogeneous distribution of magnetic fields in a sizeable volume fraction of all samples studied. The growth in the volume of the magnetic regions is halted by the onset of superconductivity at the critical temperature Tc. Upon further cooling, slow fluctuations of the local fields persist until a disordered spin frozen state appears below about one tenth of Tc. The μSR results are consistent with the formation of magnetic clusters in UTe2 due to the influence of disorder on long-range electronic correlations or geometrical magnetic frustration associated with the ladder-like U sublattice structure. Our findings suggest that inhomogeneous magnetic clusters are responsible for the ubiquitous residual linear term and low-temperature upturn in the temperature dependence of the specific heat in UTe2 below Tc. The omnipresent magnetic inhomogeneity has implications for the interpretation of other low-temperature experimental observations in the superconducting state of UTe2.
Conversations between earthquakes: Dynamics and delays of the 2019 Ridgecrest rupture sequence
Authors: Gabriel Alice-Agnes , Taufiqurrahman Taufiq , Li Duo , Ulrich Thomas , Li Bo , Carena Sara , Verdecchia Alessandro , Gallovič František
Published: September 2022

The overwhelming observational difficulties and the complexity of earthquake physics have rendered seismic hazard assessment largely empirical. Despite increasingly high-quality geodetic, seismic, and field observations, data-driven earthquake imaging yields stark differences and physics-based models explaining all observed dynamic complexities are elusive.
Here we present data-assimilated 3D dynamic rupture models which untwine California's biggest earthquakes in more than 20 years: the moment magnitude (Mw) 6.4 Searles Valley and Mw7.1 Ridgecrest, California, sequence breaking multiple segments of the same fault system. Our models use supercomputing to find the link between the two large earthquakes. We unify the uniquely high-quality strong-motion and teleseismic, field mapping, high-rate GNSS, and space geodetic foreshock and mainshock datasets with earthquake physics.
We find that the regional structure, the ambient long- and short-term stress, as well as the dynamic and static fault system interactions, are conjointly crucial to understand the dynamics and delays of the sequence. Dynamic rupture of a statically strong yet dynamically weak fault system is driven by overpressurized fluids and low dynamic friction in our models. The observed earthquake complexity results from static and dynamic stress changes acting across a non-vertical quasi-orthogonal conjugate fault structure.
We demonstrate that joint physics-based and data-driven illumination of the mechanics of complex fault systems and earthquake sequences is possible when reconciling dense earthquake recordings, 3D regional structure and stress models. We foresee that physics-based interpretation of big observational data-sets characterizing complex nonlinear systems will have a transformative impact on future geohazard mitigation.
Information and communication technology-based interventions for suicide prevention implemented in clinical settings: A scoping review
Authors: Shin Hwayeon Danielle , Durocher Keri , Sequeira Lydia , Zaheer Juveria , Torous John , Gillian Strudwick
Published: September 2022

Background
There is a surplus of information communication technology (ICT) based interventions for suicide prevention. However, little is known about which of these ICTs are implemented in clinical settings and their characteristics. This scoping review aimed to map and characterize evidence of ICTs for suicide prevention implemented in clinical settings. Furthermore, this review identified and characterized implementation barriers and facilitators, evaluation outcomes, and measures.
Methods
We conducted this review following the Joanna Briggs Institute methodology for scoping reviews. A search strategy was completed using the following databases between August 17–20, 2021: MEDLINE, Embase, CINAHL, PsycINFO, Web of Science, and Library, Information Science and Technology Abstracts. We also supplemented our search with Google searches and scanning of reference lists of relevant reviews. We used the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews to report our findings.
Results
This review included a total of 75 articles, describing 70 studies and 66 ICTs for suicide prevention implemented in clinical settings. The majority of ICTs were computerized interventions and applications (n = 55). These ICTs were commonly used as indicated strategies (n = 49) targeting patients who were actively presenting with suicide risk. The three most common suicide prevention intervention categories identified were post-discharge follow-up (n = 27), screening and assessment (n = 22), and safety planning (n = 20). A paucity of reported information was identified related to implementation strategies, barriers and facilitators. The most reported implementation strategies included training, education, and collaborative initiatives. Barriers and facilitators of implementation included the need for resource supports, knowledge, skills, motivation as well as engagement with clinicians with research teams. Studies included outcomes at patient, clinician, and health system levels, and implementation outcomes included acceptability, feasibility, fidelity, and penetration.
Conclusion
The findings from this review illustrate several trends of the ICTs for suicide prevention in the literature and identify a need for future research to strengthen the evidence base for improving implementation. More effort is required to better understand and support the implementation and sustainability of ICTs in clinical settings. The findings can also serve as a future resource for researchers seeking to evaluate the impact and implementation of ICTs.
Constrained parameter-splitting perturbation method for the improved solutions of nonlinear vibrations of Euler-Bernoulli cantilever
Authors: Du Hai-En , Er Guo-Kang , Iu Vai Pan , Li Li-Juan
Published: September 2022

In this paper, a new method named constrained parameter-splitting perturbation method for improving the solutions obtained from the parameter-splitting perturbation method is proposed for solving the problems in some extremal cases, such as the strongly nonlinear vibration of Euler-Bernoulli cantilever. The proposed method takes the advantages of both the perturbation method and the harmonic balance method. The idea is that the solution obtained by the parameter-splitting perturbation method is substituted into the equation of motion and then the accumulative error of the equation is minimized for determining the unknown splitting parameters under the constrains constructed under the frame of harmonic balance method. The forced vibration of an oscillator with cubic geometric nonlinearity and inertia nonlinearity and the forced vibration of a planar micro cantilever beam with a lumped tip mass are studied as examples to reveal the efficacy of the proposed method. The inspection of the steady-state response is conducted by means of comparing the frequency-response curves obtained by the proposed method with those obtained by the numerical continuation method and harmonic balance method, respectively, to show the efficacy and the advantages of the proposed method. Meanwhile, the nonlinear ordering effect on the solutions of the proposed method is also studied by comparing the results obtained by using different nonlinear orderings in the systems.
Research on Flow experience and social influences of online short video users. An example of TikTok.
Authors: Cheng Zheng
Published: September 2022

Recently, the online short video has been highly recognized by the video market users, and then developed rapidly. This study aims to explore why users enjoy watching and sharing online short videos by applying the theory of flow experience. Previous research extensively examined traditional video arts such as TV and movies, and social media platforms that are text- or image-based, research on the online short video has been only surging in the recent years. In order to improve the preciseness and comprehensiveness of the research, social influence is also used as variables. This study takes short video representative platform TikTok as an example, and through questionnaires, 406 users' data about online short video experience were collected. After statistical analysis, the study finds that flow experience has a significant impact on the Follow and Share of online short video. According to further analyses, the research finds that flow experience, social norms, perceived critical mass and the Follow and Share constitute three groups of mediating relationship. Finally, through the discussion of the research results, it also provides help for broadening the academic scope of flow experience and video art, improving the online short video platform environment, and upgrading online short video services .
Co-pyrolysis of rice husk and cattle manure in N2/CO2 atmosphere
Authors: Liu Jiacheng , Qiu Wen , Ding Yongyu , Cheng Qunpeng , Fan Guozhi , Song Guangsen , Cui Baihui
Published: September 2022

The co-pyrolysis of rice husk and cattle manure was carried out in N2/CO2 atmosphere in a fixed pyrolysis furnace. The effects of pyrolysis temperature and ratio of rice husk to cattle manure on gas yield were studied. Thermo-gravimetric analyzer was used to explore the influence of rice husk on the pyrolysis process of cattle manure. Meanwhile, tar was analyzed by GC-MS to investigate the co-pyrolysis reactions of cattle manure with rice husk. The results showed that the co-pyrolysis of rice husk and cattle manure displayed a good synergistic effect where the mass loss rate was increased by 5.01%. It could significantly improved hydrogen production. Highest H2 content (26.34%) was obtained at 800℃ with the ratio of 1:0. Co-pyrolysis reduced the content and types of PAHs in tar, while increased the content of aliphatic hydrocarbons and oxygen-containing aliphatic hydrocarbons (ketones). Compared with N2 atmosphere, the contents of H2 and CO at CO2 atmosphere were increased by 30.25% and 30.69% respectively at 800℃ with the ratio of 1. At CO2 atmosphere, the pyrolysis of organic matter was promoted which resulted in a decrease of polycyclic aromatic hydrocarbons content and increase of aliphatic hydrocarbons and oxygen-containing aliphatic hydrocarbons content.
Environmental and economic performance investigation of natural gas and methanol as a marine alternative fuel.
Authors: Nasser Mahmoud Abdel , Elgohary Mohamed M. , Abdelnaby Maged , Shouman Mohamed R.
Published: September 2022

Emissions from ships are a serious global issue due to their effects on environmental damage, particularly global warming of the atmosphere. As a result, the International Maritime Organization (IMO) places a high priority on environmental protection by reducing exhaust emissions by at least 50% by 2050. Among the IMO's proposed measures, using alternative marine fuels such as natural gas and methanol instead of conventional fuels has been prioritised. In this paper, a comparative study between convert diesel engine into dual fuel engine operated with alternative fuels such as methanol or natural gas is carried out. Environmental and economic assessment of the natural-dual fuel engine and methanol- dual fuel engine is conducted. A13-class container ship is investigated as a case study. The evaluation results show that using natural gas in a dual fuel engine with a percentage (95% NG and 5% MDO) reduces NOx, SOX, CO2, PM, and CO pollutions by 83%, 95%, 19.4%, 95%, and 32.6%, respectively, while the emissions percentage will be 81.2%, 95%, 57.1%, 95%, and 58.4%, in order, when using methanol as a dual fuel with percentage 95% Methanol. Moreover, the cost-effectiveness of using natural gas was 769.25 $/ton, 3304.1$/ton, 81.2 $/ton. 60082.64 $/ton, and 23782.84 $/ton for NOx, SOx, CO2, PM, and CO, in the order, while for methanol, was 850.67 $/ton, 3340.1$/ton, 45.588 $/ton, 55450.87 $/ton, and 13274.11 $/ton, respectively.
Increased Risk of Group B Streptococcal Meningitis in Hiv-exposed Uninfected Infants in a High-Income Country
Authors: Manzanares Á. , Tato L. M. Prieto , Escosa-García L. , Navarro M. , Guillén S. , Penin M. , Hernanz-Lobo A. , Sánchez B. Soto , Mosquera J. Beceiro , Romero I. Falces , Amador J.T. Ramos , Miguel M.A. Orellana , Epalza C.
Published: September 2022

Purpose
to compare group B Streptococcus (GBS) infection incidence in HIV-exposed uninfected (HEU) and HIV-unexposed (HU) infants in a Spanish cohort.
Methods
retrospective study in 5 hospitals in Madrid (Spain). Infants ≤ 90 days of life with a GBS infection were included from January 2008 to December 2017. Incidence of GBS infection in HEU and HU children was compared.
Results
HEU infants presented a 7-fold greater risk of GBS infection and a 29-fold greater risk of GBS meningitis comparing to HU, with statistical signification. Early-onset infection was 10-fold more frequent in HEU children, with statistical signification, and late-onset infection was almost 5-fold more frequent in the HUE infants’ group, without statistical significance.
Conclusions
HEU infants presented an increased risk of GBS infection and meningitis. One in each 500 HEU infants of our cohort had a central nervous system infection and 1 in each 200, a GBS infection. Although etiological causes are not well understood, this should be taken into account by physicians when attending this population.
Structure of the catalytically active APOBEC3G bound to a DNA oligonucleotide inhibitor reveals tetrahedral geometry of the transition state
Authors: Maiti Atanu , Hedger Adam , Myint Wazo , Balachandran Vanivilasini , Watts Jonathan , Schiffer Celia , Matsuo Hiroshi
Published: September 2022

APOBEC3 proteins (A3s) are enzymes that catalyze deamination of cytidine to uridine in single-stranded DNA (ssDNA) substrates, thus playing a key role in innate antiviral immunity. However, APOBEC3 family has also been linked to many mutational signatures in cancer cells, which has led to intense interest to develop inhibitors of A3’s catalytic activity as therapeutics as well as tools to study A3’s biochemistry, structure and cellular function. Recent studies have shown that ssDNA containing 2′-deoxy-zebularine (dZ-ssDNA) is an inhibitor of A3s such as A3A, A3B and A3G, although atomic determinants of this activity remained unknown. To fill this knowledge gap, we determined a 1.5 Å resolution structure of a dZ-ssDNA inhibitor bound to active A3G. The crystal structure revealed that the activated dZ/H2O mimics the transition state by coordinating the active site Zn2+ and engaging in additional stabilizing interactions, such as the one with the catalytic residues E259. Therefore, this structure allowed us to capture the first snapshot of the A3’s transition state, and suggests that developing transition-state mimicking inhibitors may provide a new opportunity to design more targeted molecules for A3s in the future.
Clinicopathologic characteristics of early-onset breast cancer: A comparative analysis of cases from across Ghana
Authors: Akakpo Patrick Kafui , Imbeah Emmanuel Gustav , Edusei Lawrence , Naporo Simon , Ulzen-Appiah Kofi , Clegg-Lamptey Joe Nat , Dedey Florence , Nsaful Josephine , Affram Nelson , Wiafe Beatrice , Mensah Samuel , Nortey Michael , Sheriff Mohammed , Amponsah-Manu Forster , Agbedinu Kwabena , Jiagge Evelyn Mawunyo
Published: September 2022

Background: Breast cancer is the commonest cancer diagnosed globally and the second leading cause of cancer-related mortality among persons younger than 40 years. This study comparatively reviewed the demographic, pathologic and molecular features of Early-Onset Breast Cancer (EOBC) reported in Ghana in relation to Late Onset Breast Cancer (LOBC). Methods: A descriptive, cross-sectional design was used, with purposive sampling of retrospective histopathology data from 2019 to 2021. Core/incision biopsy, mastectomy and matched immunohistochemistry reports were merged into a single file and analysed with SPSS v. 20.0. Descriptive statistics of frequencies and percentages were used to describe categorical variables. Cross-tabulation and chi-square test was done at a 95% confidence interval with significance established at p<0.05Results: A total of 2,418 cases were included in the study with 20.2% (488 cases) being EOBCs and 79.8% (1,930 cases) being LOBCs. The median age at diagnosis was 34.66 (IQR: 5.55) in the EOBC group (<40 years) and 54.29 (IQR: 16.86) in the older, LOBC group (>/40 years). Invasive carcinoma - No Special Type (NST) was the commonest tumour type with grade III tumours being the highest recorded tumour grade in both age groups. Perineural invasion was statistically significant with age. EOBC was associated with higher DCIS component (24.8% vs 21.6%), lower hormone-receptor-positive status (52.30% vs 55.70%), higher proliferation index (Ki-67>20: 82.40% vs 80.30%) and a higher number of involved lymph nodes (13.80 vs 9.00%). Triple-Negative Breast cancer (26.40% vs 24.30%) was the most predominant molecular subtype of EOBC. Conclusion: EOBCs in our setting are generally more aggressive with poorer prognostic histopathological and molecular features when compared with LOBCs. A larger data set is needed to explain the lack of statistical significance of pathological features. Further molecular and genetic studies to understand the molecular genetic drivers of the general poorer outcome of EOBCs in our setting is recommended.
Dissipative control of switched nonlinear systems with sliding mode: an event-triggered control scheme
Authors: Dong Xiaoxiao , Zhang Xi
Published: September 2022

This paper introduces the event-triggered scheme to study the problems of dissipative control and dissipativity-based sliding mode control (SMC) for switched nonlinear systems. Firstly, based on a designed event-triggered scheme, a piecewise continuous state feedback controller is constructed, sufficient conditions for (Q,S,R)-α-dissipativity of the resulting system are derived by the approaches of a switched Lyapunov function and an average dwell time. Secondly, these two methods are extended to the study of H∞ performance and passive performance, the corresponding sufficient conditions are provided. Thirdly, the dissipative control problem is solved by introducing an event-triggered scheme into SMC method. Sufficient conditions for dissipativity of the resulting system are derived and an SMC law is designed to guarantee the reachability. At the end of this paper, the correctness of the proposed methods is proved by two numerical examples.
Comparison of bird assemblage structures and diversity patterns between seasons among the three Ethiopian wetlands
Authors: Awash Numeri , Tekalign Wondimagegnehu
Published: September 2022

Wetlands are significant habitats for avian populations, and knowledge of the diversity and other ecological aspects of bird species contribute to the management of the ecosystem. The present study was based on comparative studies of the diversity and relative abundance of bird species in the three wetlands of southwest Ethiopia. The point count method was utilized in this study. For the data analysis, the Shannon-Wiener diversity index, independent sample t-test, and similarity index were employed. A total of 46 bird species under 11 orders and 30 families were identified. The species diversity and relative abundance were higher in all three wetlands during the wet season. The Loga wetland had the highest diversity (H’ = 3.089), whereas the lowest diversity (H’ = 2.643) was recorded in the wetland of Hurri. During the dry season, the highest and the lowest diversity were also recorded in the Loga wetland (H’ = 2.738) and the Hurri habitat (H’ = 2.283), respectively. Seasonal differences in the species diversity of bird species are not statistically significant (p > 0.05). Since the existence of bird species is reliant on a wetland ecosystem; human activities very close to the wetland should be controlled for their sustainable conservation.
Comprehensive analysis to identify long non-coding RNA HCP5/MICA axis as a prognostic biomarker in glioblastoma
Authors: Qin Qilin , Mao Wenbin , Zhang Xuezhi , Wang Xinzhuang
Published: September 2022

Purpose
To explore the lncRNA-mRNA network based on the IDH1 mutation status and construct a prognostic model for GBM.
Methods
The data of expression, somatic mutation, methylation, immune microenvironment were obtained from TCGA database, CGGA database and GTEx database. The common differential expressed genes (DEGs) between IDH1 mutant GBM and IDH1 wildtype GBM were used to perform a series of bioinformatics analysis to identify the hub gene. LASSO cox regression was used to screen a risk signature and nomogram was used to construct a prognostic model for GBM.
Results
LncRNA HCP5/MICA axis was downregulated in IDH1 mutant GBM and associated with the overall survival (OS) of GBM patients. Further analysis revealed that MICA expression was positively correlated with immune infiltration as well as the expression of several immune checkpoint gene in GBM. We screened a risk signature using MICA-associated genes by LASSO cox regression and the risk score performed well in predicting the OS. Furthermore, the risk score was used to construct a nomogram prognostic model combined with age, gender, IDH mutation, TP53 mutation, radiation and chemotherapy to predict the OS at 1-year, 2-year and 5-year.
Conclusions
This study constructed a prognostic model for GBM with MICA and clinical data for the first time and provided an insight into the molecular therapy of GBM. Further studies are required to uncover the mechanism of regulation between HCP5 and MICA in GBM.
Does knowledge acquisition transform clinical practice? Continuing medical education designed for knowledge improvement can significantly alter diagnoses and care maintenance across specialties: A randomized study of statistical inference
Authors: Ruggiero John , Meo Antonio B. , Bishay M. Theresa , Azzi Brigitte , Urhoghide Osarumen , Malnarick Gail
Published: September 2022

Background
Accredited continuing medical education contributes to the acceleration of scientific information into practice. Additional evidence confirming an ongoing debate that knowledge-based education directly results in practice change, apart from education designed specifically for Quality Improvement, would however complement the current inventory of literature related to the impact of continuing education. This study therefore examined if reinforcing multiple knowledge-building continuing medical education activities lead to or correlates with clinical confidence and rates of practice change.
Methods
From March 17, 2022, to May 11, 2022, a randomized sample of U.S. healthcare professionals (N = 500), including but not limited to physicians, nurses, and pharmacists working across specialties and practices, provided their perspectives on accredited educational activities they completed within the previous six months. Participants then matched their practice notes to recommendations made within the education.
Results
75.3% documented enhanced personalized and proper diagnoses, enhanced diagnostic test orders, and alternative care plan implementation. A Z-test compared this average to that of previous research (33%). A directional hypothesis test with 0.5 alpha level of significance led to hypothesis acceptance with 95% confidence. Additional Pearson correlation testing indicated a positive relationship between education and diagnostic test orders, proactive adverse event management, and personalized care plan changes (r = 0.55, 0.64, and 0.66, respectively), with positive linear regression (r2 = 0.30, 0.41, and 0.44, respectively). When clustered by practice setting and time in practice, there were no statistical differences in the results.
Conclusions
Accredited continuing education, designed for knowledge-acquisition, can lead to practice change across clinical specialties and practices, irrespective of a clinician’s time in practice. This research is meant to generate hypotheses and continued discourse within the clinical and educational communities.
Mapping of quantitative trait loci (QTLs) controlling cotton leaf curl disease (CLCuD) resistance in Upland cotton
Authors: Sattar Muhammad N. , Javed Muhammad , Hussain Syed B. , Babar Muhammad , Chee Peng W. , Iqbal Zafar , Munir Muhammad , Al-Hashedi Sallah A.
Published: September 2022

Cotton leaf curl disease (CLCuD), caused by begomoviruses, is a major threat to cotton (Gossypium hirsutum L.) production, especially in Asia and Africa. The use of marker-assisted breeding to utilize host plant resistance can be the best sustainable approach to tackle CLCuD. Because most of the available cotton germplasm is susceptible to CLCuD, so it demands the identification of potentially new quantitative trait loci (QTLs) in the indigenous germplasm. The current study was designed to construct a broader genetic linkage map of bi-parental F2:F3 populations developed from a highly tolerant MNH 886 and a highly susceptible S-12 cotton cultivars. One hundred seven (107) CLCuD-associated simple sequence repeat (SSR) marker alleles were identified as polymorphic and eight new QTLs were found on chromosomes 11, 19 and 21. On homologous chromosomes 11 and 17, the QTLs qCL-11-120d2 and qCL-21-m2 were detected in both F2 and F3 populations. Another QTL on chromosome 11, qCL-11-m3, showed the highest phenotypic variance (PV%) of 16.66% and LOD score > 3. Other QTLs on chromosome 11 were qCL-11-m2, qCL-11-120d3. On chromosome 19, three QTLs (qCL-19-m2, qCL-19-90d2 and qCL-19-120d3) were detected with PV% >8 and LOD score > 3. The CLCuD-associated QTLs identified in this study can contribute to develop CLCuD-resistant cultivars using marker assisted selection and can help to fine-tune the molecular mapping of the QTLs on the cotton genome.
High Ki67 expression before docetaxel, cisplatin, and fluorouracil (TPF) neoadjuvant chemotherapy predicts better prognosis in patients with locally advanced oral squamous cell carcinoma
Authors: Zhou Xin-yu , Chen Gang , Zhao Tong-chao , Yao Yu , Zhang Zhi-yuan , Zhou Zhi-hang , Zhong Lai-ping , Zhu Dong-wang
Published: September 2022

Background Patients with locally advanced oral squamous cell carcinoma (OSCC) failed to benefit from docetaxel, cisplatin and 5-fluorouracil (TPF) neoadjuvant chemotherapy prior to surgery and postoperative radiotherapy in our previous phase 3 trial (NCT01542931). The aim of the present study was to explore the prognostic value of Ki67 expression for individualized TPF neoadjuvant therapy. Methods Immunohistochemical staining of Ki67 was performed in the biopsies before treatment in the experimental arm of our previous phase 3 trial. A total of 65 patients with Ki67 expression below 25% or above 50% were retrospectively reviewed in this study. Kaplan‑Meier analysis, log-rank test and Cox proportional hazards model were used for survival analysis. Results Among the 65 patients enrolled in the study, the patients with high Ki67 expression (n = 41) before treatment exhibited higher overall survival (OS) (P = 0.041) and distant metastasis–free survival (DMFS) (P = 0.049) than those with low expression (n = 24). The 5-year OS of the two groups were 70.7% and 45.8% respectively (high vs low). Cox proportional hazards model showed that high Ki67 expression before TPF neoadjuvant chemotherapy had a positive impact on the OS (HR = 0.399, 95% CI: 0.178–0.893, P = 0.025). Conclusions High Ki67 expression before treatment could predict the survival benefits from TPF neoadjuvant chemotherapy prior to standard therapy in patients with locally advanced OSCC, which could act as a biomarker for individualized treatment.
A disintegrin and metalloproteinase 10 (ADAM10) is essential for oligodendrocyte precursor development and myelination in the mouse brain
Authors: Guo Dazhi , Huang Fei , xue ruijun , Ma Yuehong , Xiao Lin , Lou Huifang , pan shuyi
Published: September 2022

A disintegrin and metalloproteinase 10 (ADAM10) plays an essential role in the regulation of survival, proliferation, migration, and differentiation of various neural cells. Nevertheless, the role of ADAM10 in oligodendrocyte precursors (OPCs) and myelination in the central nervous system (CNS) of developing and adult mouse brains is still unknown. We generated ADAM10 conditional knockout (ADAM10 cKO) mice lacking the ADAM10 gene primarily in OPCs by crossing NG2-Cre mice with ADAM10loxp/loxpmice. We found that OPCs expressed ADAM10 in the mouse corpus callosum and the hippocampus. ADAM10 cKO mice showed significant loss of back hair and reduction in weight and length on postnatal (30 ± 2.1) day, died at (65 ± 5) days after birth and exhibited the “anxiety and depression-like” performances. Conditional knockout of ADAM10 in OPCs resulted in a prominent increase in myelination and a decrease in the number of OPCs in the corpus callosum at P30 owing to premyelination and lack of proliferation of OPCs. Moreover, the number of proliferating OPCs and mature oligodendrocytes (OLs) also decreased with age in the corpus callosum of ADAM10 cKO mice from P30 to P60. Western blot and RT-PCR results showed that the activation of Notch-1 and its four target genes, Hes1, Hes5, Hey1, and Hey2, was inhibited in the corpus callosum tissue of ADAM10 knockout mice. In our study, we provided experimental evidence to demonstrate that ADAM10 is essential for modulating CNS myelination and OPC development by activating Notch-1 signalling in the developing and adult mouse brain.
Observing and braiding topological Majorana modes on programmable quantum simulators
Authors: Movassagh Ramis , Shtanko Oles , Harle Nikhil
Published: September 2022

Electrons are indivisible elementary particles, yet paradoxically a collection of them can act as a fraction of a single electron, exhibiting exotic and useful properties. One such collective excitation, known as a topological Majorana mode, is naturally stable against perturbations, such as unwanted local noise, and can thereby robustly store quantum information. As such,
Majorana modes serve as the basic primitive of topological quantum computing, providing resilience to errors. However, their demonstration on quantum hardware has remained elusive. Here, we show the first provable detection and braiding of topological Majorana modes using a superconducting quantum processor as a quantum simulator. By simulating fermions on a one-dimensional lattice subject to a periodic drive, we confirm the existence of Majorana modes localized at the edges, and distinguish them from other trivial modes. To simulate a basic logical operation of topological quantum computing known as braiding, we propose a non-adiabatic technique, whose implementation reveals correct braiding statistics in our experiments. This work could further serve as a superconducting circuit verification protocol for future topological quantum computation, and shows that long-sought quantum phenomena can be recreated by anyone in cloud-run quantum simulations, whereby accelerating fundamental new discoveries in quantum-related science and technology.
Laparoscopic resection vs thermal ablation for 3-5cm hepatocellular carcinoma: A multicenter study based on machine learning
Authors: Zheng Lin , Wang Zhen , Ding Wenzhen , Yu Jie , Liu Fang-yi , Cheng Zhi-gang , Han Zhi-yu , Yu Xiao-ling , Liang Ping
Published: September 2022

Background Very early recurrence (VER) significantly affects the survival of patients with treated primary hepatocellular carcinoma. Purpose To compare the survival of patients with VER after laparoscopic resection and thermal ablation treatment. To establish a model predicting very early recurrence after laparoscopic resection. Methods 956 primary HCC patients treated with thermal ablation or laparoscopic resection from 12 centers were included in our study. The overall survival (OS) and cancer specific survival (CSS) rate of all patients and patients with VER in two groups were compared. Propensity score matching (PSM) was done to keep baseline balance. Risk factors that may lead to VER occurrence were presented. Different machine learning methods were used to build prediction models of VER occurrence after laparoscopic resection. Results VER significantly affects the OS and CSS of patients in both groups(p < 0.0001). But OS and CSS of patients with VER in two groups has no statistical difference before (p = 0.39 and 0.27, respectively) or after PSM (p = 0.4 and 0.85, respectively). A model predicting the occurrence of VER after laparoscopic resection was established based on machine leaning with 5 factors (AFP, ALT, TBil, DBil and number of tumors) included, which had the highest AUC value of 0.722. Conclusion 3-5cm primary hepatocellular carcinoma patients with VER after laparoscopic resection can benefits more if choose thermal ablation instead. And patients with VER after laparoscopic resection can be reliably predicted by the model presented in this study.
Design, Photochemistry, Logic Gates Behavior and Antibacterial Evaluation of novel ICT systems based on 1,8- naphthalimides
Authors: Sakr Alaa , Georgiev Nikolai , Bojinov Vladimir
Published: September 2022

Ace Naphthene as a primary material was nitrated and further reacted by various unit processes such as oxidation, imidation and amination in order to obtain dyad 5. The Dyad 5 fluorescence in solvents of different polarity is shifted bathochromically with increasing the solvent polarity due to ICT enhancement, fluorescent enhancement is greatest in case of chloroform (ФF = 0.48) Excitation at 420 nm ,The influences of the pH on dyad 5 were also investigated, the results can be considered as representing off-on-off states. dyad 5 executes two input XNOR logic gates, the dyad 5 shows optical sensing towards H+ and HO− ions, it was investigated the “off-on-off” switching behavior of the receptor between H+ and HO− ions, the synthesized compounds were tested for antimicrobial activity of Gram-positive and Gram-negative bacteria, compounds were tested have a good antibacterial activity as compared with standard compound (Gentamycin).
Environmental factors drive bacterial community (free-living vs particle-attached) assembly and more stable network in the pre-bloom period than post-bloom
Authors: Ren Junming , Ye Jinliu , Cui Xinyu , Zhang Xiaofeng , Lang Chengyu , Xie Wenming , He Huan , Meng Han , Wang Guoxiang , Zhang Limin
Published: September 2022

Cyanobacterial blooms are very common phenomenon in freshwater ecosystems, influencing the composition and function of bacterioplankton communities. However, the drivers on the pattern and mechanism of bacterial community were still little known especially before and after bloom. Here, high-throughput sequencing of bacterial 16S rRNA gene was used to investigate the dynamics of communities with two lifestyles of free-living (FL, 0.22-3 μm) and particle-attached (PA, > 3 μm) in the Lake Taihu, and co-occurrence patterns and assembly mechanism of bacteria were also explored between pre-bloom and post-bloom periods. The results exhibited that the bloom event significantly changed the composition and diversity of plankton community. Primarily, phyla of Proteobacteria, Actinobacteria and Bacteroidetes were dominated in FL bacteria, whereas Proteobacteria, Cyanobacteria and Bacteroidetes were distributed in PA bacteria. Additionally, PA bacteria had higher α-diversity than FL bacteria in two periods. Co-occurrence network analysis revealed that the network was more complex interactions and modularity in the pre-bloom than post-bloom. In addition, multispecies cooperation with negative correlations in the network implied more contribution to the stability and resilience of community in the pre-bloom. Null-model analysis indicated homogeneous selection was the key assembly process in the pre-bloom, while stochastic processes played predominant role in the post-bloom. Moreover, abiotic factors were significantly correlated with keystone taxa to further reveal the deterministic process in the pre-bloom ( p < 0.05). Overall, these findings indicate the ecological network and assembly mechanisms of bacteria driven by environmental factors in the pre-bloom more than post-bloom in a changing aquatic niche.
LC3-associated phagocytosis promotes glial degradation of axon debris after injury
Authors: Szabo Aron , Vincze Virag , Bognar Sarolta , Varga Katalin , Banik Poulami , Jipa Andras , Neukomm Lukas , Juhasz Gabor
Published: September 2022

Glial engulfment of dead neurons and neurites after trauma, during development and in neurodegenerative diseases plays a crucial role in nervous system maintenance. Axon debris generated after traumatic injury is cleared by phagocytic glia via Draper receptor signalling in Drosophila. However, mechanisms governing the efficiency of axon debris phagocytosis and degradation have remained largely unexplored. Here we show that glial LC3-associated phagocytosis (LAP), an engulfment pathway assisted by certain components of the macroautophagy machinery, promotes clearance of degenerating axons in the Drosophila wing nerve. A LAP-specific subset of autophagy-related (Atg) genes is required in glia for efficient debris elimination, which includes members of the Atg8a (LC3) conjugation system and the Vps34 lipid kinase complex subunits UVRAG and Rubicon but not Atg14 or the Atg1 kinase complex. Atg8a and Rubicon are recruited to Rab7-positive phagosomes and Atg8a lipid conjugation is essential for debris-containing phagosome maturation. Finally, Rubicon overexpression in glia accelerates axon debris elimination. Our results reveal the critical role of LAP in glia in the clearance of neuronal debris in vivo, with important implications for the recovery of the injured nervous system.
Effect of Combined Exercise Training Physical and Functional Capacity in Post-Covid Patients
Authors: Costa Bruno Margueritte , Sochodolak Rafael Carlos , Antunes Edher Lucas , Okuno Nilo Massaru
Published: September 2022

The purpose of this study was to investigate the effects of supervised moderate combined exercise training in patients with post-COVID in the physical and functional capacity. Forty-one patients completed 6-week combined moderate exercise training. In baseline and after intervention, patients were assessments of screening, including clinical data and anthropometrics performed functional tests, which consisted of handgrip strength test, chair sit and reach test, arm curl test, 30-sec sit to stand test, timed up and go, and six-minute walk test (6MWT). Primary outcomes were results of functional tests, and secondary outcomes were clinical data. Handgrip strength (p < 0.001), flexibility (p = 0.01), strength of upper (p = 0.01) and lower limbs (p < 0.001), gait speed (p < 0.001) and 6MWT (p < 0.001) improved after rehabilitation. Resting heart rate, systolic and diastolic blood pressure were lower after intervention (p = 0.01; p = 0.05; p = 0.03, respectively). No one difference was observed in persistent symptoms (p > 0.05). In conclusion, exercise training promoted great physical, functional and cardiovascular benefits for post-COVID patients.
Anatomy of the Nasal Latero-lateral Cartilage Articulation: A Micro-MRI Study in Human Specimens.
Authors: HADDAD Axel , DODIN Gauthier , HOSSU Gabriela , PEREZ Manuela , TEIXEIRA Pedro Augusto GONDIM , RUMEAU Cecile , JANKOWSKI Roger , GILLET Romain
Published: September 2022

Purpose: To study the anatomy of the latero-lateral joint (LLJ) between the upper lateral (ULC) and lower lateral (LLC) crus of the nasal cartilages, usually described as a scroll articulation.
Methodology: Six nasal pyramids were taken in monobloc from fresh cadavers and imaged on micro-MRI with 0.4 mm slice thickness. Images were jointly interpreted by two head and neck radiologists and one surgeon. The junction between the ULC and LLC, the presence of ligaments and of sesamoid or accessory cartilages were assessed.
Results: Eight LLJs could be analyzed, with 4 types of junctions: hook-shaped cephalic border of the LLC turned towards the nasal fossa and linear caudal border of the ULC (n=3), hook-shaped caudal border of the ULC and linear cephalic border of the LLC lateral crus (n=1), hook-shaped border of both cartilaginous edges with clinging (n=1) (scroll articulation) or without clinging (n=3). No ligament or sesamoid cartilage was found, but posterior accessory cartilages were seen in 75% of the cases.
Conclusion: The classical scroll articulation of the LLJ has been observed in only 1/8 cases on micro-MRI images. The anatomy of the LLJ couldexplain the surgical difficulty in raising the tip of the nose in some patients and not in others.
Cervical catheter placement leads to improved rostral distribution of a radiolabeled 18F-baclofen analog in cynomolgus monkeys
Authors: Duclos Brian A , Roegge Cindy , Dobson Howard , Haller Scott , Bodner Jeff , Pannem Sanjana , Gesaman Janelle , Nourmohammadi Amin
Published: September 2022

Background:
Intrathecal (IT) catheter delivery of baclofen via continuous infusion using an implantable pump is an important means of treating patients with severe spasticity. We evaluated the impact of IT catheter tip placement (upper vs. lower) on brain and spine distribution of a radioactive tracer molecule.
Methods:
Cynomolgus monkeys were implanted with an IT catheter, with the distal tip located at either C1 or T10 and attached to an implanted continuous infusion pump. A radioactive tracer molecule, an 18F-baclofen analog, and PET imaging were utilized to observe tracer distribution and quantitate levels of tracer in both the brain and spine according to catheter tip location.
Results:
It was consistently determined that a high cervical (C1) catheter tip placement resulted in both more rapid distribution and higher concentrations of radiotracer in the brain and upper spine compared with lower thoracic (T10) during the first 6 hours of infusion.
Conclusions:
These results indicate that delivery of 18F-baclofen by IT catheter results in repeatable proportional distribution within regions of the brain and spine. The data also suggest that the greatest exposure to the brain and cervical spinal cord occurs when the catheter tip is located at the first cervical vertebra.