bluuebunny/crossref_metadata_2025 huggingface dataset:
REVIEW OF SUPPLIER EVALUATION CRITERIA IN SUSTAINABLE SUPPLY CHAIN MANAGEMENT BY DEMATEL METHOD AND AN APPLICATION IN THE WOOD SECTOR
Authors: COŞKUN Birol , YILDIZ Mehmet Selami , BAYRAKTAR Merve
Published: June 2022

Supplier selection is considered among the most criterional decision-making problems as it aims to maximize conflicting targets. In order to meet the demands of customers, enterprises take environmental and social factors into account as well as economic factors. The main objective of the study is to evaluate supplier selection criteria within a sustainable supply chain and to determine which criteria have more weight. In the study, a conceptual framework has been developed by taking into account economic, environmental and social factors in order to evaluate the performance of a sustainable supply chain performance. In addition to these 3 main criteria, 9 sub-criteria are also included in the study. In this study, the DEMATEL method is applied to measure the sustainability performance of suppliers in the sustainable supply chain of a company operating in the natural wood coating sector. At the end of the practice, the weight rating of each criterion is calculated. It has been revealed that the economic, then social and environmental dimension is considered important in the sustainable supplier selection criteria for the company evaluated within the scope of the study and that the cost of supply is the most important performance criterion. The criteria are divided into affecting and affected by evaluating the level of importance relative to each other. It has been shown that the enterprise should focus on which performance criteria in the supplier selection.
Prophylactic Laser Treatment Posterior to Pars Plana Vitrectomy Sclerotomy Wounds during Macular Surgery
Authors: Ajlan Radwan , Pfannenstiel Matthew , Luvisi Joseph
Published: June 2022

Introduction. Sclerotomy related retinal breaks (SRRBs) are a risk factor for postoperative Retinal detachment (RD). Endolaser posterior to sclerotomy wounds decreased the risk of SRRBs after 20G pars plana vitrectomy (PPV) for macular disease. However, similar data does not exist for 25G and 23G wounds.
Methods. Retrospective cohort study of patients after 23G and 25G PPV for macular pathology between August 2017 and August 2020. The primary outcome was the postoperative rate of SRRBs or RDs. The secondary outcome was the postoperative rate of pupillary dysfunction and neurotrophic keratopathy. All participants had a minimum postoperative follow up of one year.
Results. 117 patients were included in the study (62 in the laser group, and 55 in the control group). Mean age was 65.4±11.3 years (56.4% female and 43.6% male). Most of the laser group underwent 23G PPV (90%) while most of the control group underwent 25G PPV (96%). One patient in the control group developed RD secondary to a SRRB. No SRRBs or RDs developed in the laser group. None of the secondary outcomes developed in either group after one year.
Conclusions. To the best of our knowledge, this is the first report in the literature on prophylactic laser posterior to small gauge sclerotomies (25G and 23G) during macular surgery. Laser treatment posterior to small gauge sclerotomies (25G and 23G) had a similar incidence of SRRBs as with 20G sclerotomies. Larger prospective studies are needed to further understand the role of laser in lowering SRRB risk.
Rodeo Trauma: Outcome Data from 10 Years of Injuries
Authors: Seifert Clinton , Rogers Mark , Helmer Stephen , Ward Jeanette , Haan James M
Published: June 2022

Introduction.  There is little data addressing rodeo injury outcomes, though injury incidence is well described.  The purpose of this study was to describe rodeo-related injury patterns and outcomes.
Methods.  A 10-year retrospective case series was performed of patients injured in rodeo events and who were treated at an ACS-verified Level I trauma center.  Data regarding demographics, injury characteristics and outcomes were summarized.
Results.  Seventy patients were identified.  Half were injured by direct contact with rodeo stock, 34 by falls.  Head injuries were most common, occurring in 38 (54.3%).  Twenty injuries (28.6%) required surgery.  Sixty-nine patients (98.6%) were discharged to home.  There was 1 death.
Conclusions.  Head injuries were the most common injury among this cohort.  With the exception of one fatality, immediate outcomes after injury were good, with 98.6% of patients dismissed home.  Improved data collection at the time of admission may help to evaluate the success of current safety equipment use.
Virtual adaptation of resident I-PASS training session during COVID-19
Authors: Rouse Michael , Abebe Abebe , Naylor David , Gibson Cheryl
Published: June 2022

Introduction.  Effective communication during the patient handoff process is critical for ensuring patient safety. At our academic medical center, first-year interns complete hand-off training before starting clinical rotations. The purpose of this study was to evaluate a virtual handoff training for residents as an alternative to in-person sessions due to limitations imposed by COVID-19.   
Methods.  Fifty residents were administered pre/post surveys to gauge the helpfulness of the training for clinical practice, familiarity and confidence in providing a handoff, and whether they would recommend the virtual format for incoming interns. Additionally, faculty rated the virtual form of the handoff activity, made comparisons to in-person sessions, and assessed the helpfulness of the session for residents in clinical practice. 
Results.  Forty-four residents (88%) and 11 faculty (85%) completed surveys. After the training session, residents who received instruction and feedback reported significant improvements in familiarity with the handoff tool and confidence in their handoff abilities (both p< 0.001). Both residents and faculty were satisfied with the virtual format of handoff training. Most faculty felt the virtual platform was comparable to in-person sessions and would recommend ongoing use of the virtual platform when in-person sessions were not possible. 
Conclusions. Teaching hospitals mandate resident training to include strategies for a uniform handoff method to avoid medical errors. Adaptation to a virtual platform can be a successful instruction strategy, allowing for didactic and interactive sessions with direct faculty observation and feedback.
Como a Ficção Pós-Colonial Pode Contribuir Para uma Discussão Sobre Reparação Histórica: Leitura de As Telefones (2020) de Djaimilia Pereira de Almeida
Authors: Rendeiro Margarida
Published: June 2022

A literatura pós-colonial portuguesa publicada depois de 1974 deixou na obscuridade o trauma do colonizado. Os autores afrodescendentes das narrativas portuguesas publicadas desde o início da segunda década deste milénio são herdeiros da geração que fez as libertações africanas, mas que, devido aos anos subsequentes de instabilidades políticas e económicas, fazem parte da diáspora afrodescendente que cresceu em Portugal, constituindo a face visível do emaranhado cultural pós-colonial que o colonialismo produziu. Djaimilia Pereira de Almeida, autora de As Telefones (2020), que se analisa no presente artigo, é um exemplo de uma autoria e vivência afrodescendente que tanto deve às referências culturais portuguesas como à cultura angolana. Assim, argumenta-se que as narrativas de autoria portuguesa afrodescendente desestabilizam imaginários cartográficos para refletir sobre a complexidade cultural da vivência afrodescendente, contribuindo para uma polifonia ausente sobre a memória coletiva no espaço público e consequente possibilidade de reparação histórica. Sustenta-se, por um lado, que As Telefones descoloniza a experiência da perda que a literatura publicada depois de 1974 associou à memória e experiência do corpo do colonizador, mas também, e muito significativamente, ao sentimento de saudade, central na cultura portuguesa; por outro lado, defende que a centralidade narrativa do telefone como único meio de transmissão de pós-memória introduz um corte na convenção literária portuguesa que privilegia a escrita como testemunho, validando a oralidade, muito tributária para a génese das literaturas africanas.
O Ciberespaço Como Denúncia: Assédio e Discriminação Vinculados à Colonialidade no Projeto Brasileiras Não Se Calam
Authors: Lamartine Camila , Silva Marisa Torres da
Published: June 2022

O estereótipo da mulher latina é comummente associado à sexualidade. Dentre elas, as mulheres brasileiras parecem carregar ainda mais este estigma mundo fora. Enquanto imigrantes, interseccionadas por outras matrizes de opressão além de género e raça, por exemplo, estas mulheres veem-se sujeitas a diversas marcas ainda remanescentes da colonialidade eurocêntrica que as inferioriza e silencia (Mignolo, 2000/2003), o que é agravado quando o país de emigração é o seu colonizador. Este artigo explora a utilização do ciberespaço como campo de denúncia e ativismo feminista através do estudo de caso do perfil @brasileirasnaosecalam, a partir da análise de conteúdo. O projeto surge na rede social digital Instagram com o intuito de denunciar, de maneira anónima, assédios, discriminações e preconceitos que mulheres imigrantes brasileiras sofrem em Portugal, especificamente por carregarem consigo a sua própria nacionalidade. Assim, através do ciberativismo, também feminista, as mulheres dispõem de um novo ciclo político de oportunidades impulsionadas pela construção e consolidação de laços entre elas ao redor do globo, rompendo binarismos, também entre primeiro e terceiro mundo, num apelo à articulação entre fronteiras (Timeto, 2019). Constata-se a relevância de empreender uma visão ontológica que se apoie numa perspetiva feminista decolonial e interseccional que utiliza o ciberfeminismo como aliado de interconexão entre o espaço digital e o real.
Hobyar Mescidi Çinileri için Koruma Önerisi
Authors: KÖSE KOÇAK Ebru
Published: June 2022

Roma, Bizans ve Osmanlı imparatorluğuna başkentlik yapan İstanbul, kültürel zenginliği ile dünyayı her zaman cezbeden önemli bir metropoldür. Bu şehrin sahip olduğu nice esere ev sahipliği yapan en eski yerleşim yerlerinden biri Tarihi Yarımada bölgesi, tarihten bu yana biriktirdiği kültür varlıklarıyla UNESCO’nun Dünya Miras Listesi’ne girmiştir. Tarihi Yarımada; Topkapı Sarayı, Ayasofya, Sultan Ahmet Cami gibi büyük anıtlarla çevrili, ticari ve kültürel turizmin yoğun olarak yaşandığı bir bölgedir. Tarihi Yarımada’da Osmanlı ve Cumhuriyet Dönemine ait birçok çinili yapı vardır.Bu çalışmada Birinci Ulusal Mimarlık Dönemi’ne ait 20. yüzyıl başlarında Vedat Tek tarafından yapılan Hobyar Mescidinin süslemesinde kullanılan çiniler incelenmiştir. Mescidin iç ve dış cephe bezemesinde kullanılan tarihi çiniler, o dönem Kütahyalı Hafız Mehmed Emin Efendi’nin atölyesinde üretilmiştir. Hobyar Mescidinin cephelerini süsleyen tarihi çinilerin belgelemesi yapılarak kayıt altına alınması sağlanmıştır. Belgelemenin esas amaçlarını kapsayacak şekilde çiniler ile ilgili detaylı araştırmalar yapılmış ve fotoğraflar çekilmiştir. Yerinde birebir gözlem yapılarak çinilerin mevcut durumu değerlendirilmiş, bozulmalar lejantlara işlenmiştir. Çinilerin hasar tespit planları ve desenleri çizildikten sonra, onarımı ve korunması durumuyla ilgili öneriler getirilmiştir.
procesos de experimentación artística e introspectiva como oportunidad de transformación vital en la didáctica de las artes plásticas
Authors: Texeira Jiménez Rocío , Vaquero Cañestro Carmen
Published: June 2022

Este trabajo comparte los entresijos de la acción docente desarrollada en los cursos que van desde 2017 a 2020 en diversos grupos de los Grados en Educación Infantil y Educación Primaria en la Facultad de Ciencias de la Educación de la Universidad de Málaga.

La propuesta dibuja una trayectoria que conecta a modo de introducción, la crisis (un resorte que impulsa, sumerge, activa y articula de forma justificada el funcionamiento de las experiencias artísticas y docentes a lo largo de las asignaturas y que sienta la base sobre la que sucede todo), para explicitar la metodología, la consciencia (como estado de revelación y comprensión -o insight- que induce una fase de indagación artística y reflexiva ineludible) y la confesión (que es consecuencia directa del cuidado por la intimidad, protección y respeto en los ambientes y espacios físicos, mentales o emocionales, tanto individuales, como grupales y que brinda las evidencias sobre los procesos de introspección). Por último, esboza las conclusiones desde su potencialidad intrínseca, dando lugar a la oportunidad (estrategia que nos permite comprender los hallazgos y rediseñar objetivos; ésta a veces es suscitada y otras, encontrada y provoca un ciclo donde se reaviva la transformación vital, así como la observación y el análisis).

Desde esta perspectiva, la revisión de lo experimentado a partir de los hallazgos que se muestran resulta determinante en la medida en que nos permite rememorar y reconstruir lo vivido para generar un nuevo conocimiento, posibilitando la autoconciencia progresiva a un nivel personal, y por lo tanto, la mejora de la acción docente a un nivel profesional.
La arquitectura según Edward Ruscha
Authors: Fernández Morillas Antonio
Published: June 2022

La arquitectura es el medio con el que históricamente hemos transformado los paisajes naturales para generar espacios en los que desarrollarnos como seres sociales. Esta disciplina construye algunos de los símbolos culturales más representativos y, sin duda, es una de las responsables de la mediación de las personas con los lugares que habitan. Conocer y valorar las construcciones de nuestros entornos próximos nos hace ser conscientes de sus cualidades, ayudando a desarrollar una actitud más crítica con respecto a su cuidado y gestión. A pesar de no ser un contenido específico del currículum de Educación Primaria, las múltiples dimensiones de la arquitectura hacen que sus contenidos puedan ser explorados de manera transversal y multidisciplinar, adaptándose a las premisas marcadas por la educación formal. El presente estudio nos hizo indagar en las formas que definen las arquitecturas de nuestro entorno próximo, tomando nuestra casa como el punto de partida de un recorrido imaginario por el paisaje. Los participantes en las acciones artístico-educativas fueron escolares de Educación Primaria de Güejar Sierra (Granada), quedando contextualizadas por el programa de actividades para escolares de EducaUGR - Programa educativo de la Universidad de Granada. El libro de artista Every building on the Sunset Strip de Edward Ruscha (1966) nos sirvió como elemento inspirador por dos motivos esenciales: primero, por aportar un enfoque particular desde el que reconocer y representar el paisaje urbano y, segundo, por convertir un objeto cotidiano como es un libro en un soporte innovador para la creación artística. Este proyecto artístico, educativo e investigador busca transformar las prácticas educativas en Educación Primaria y considera la arquitectura como un tema de interés para la formación crítica de las personas, tomando las artes y las culturas visuales contemporáneas como un referente para el aprendizaje sobre el mundo, sus paisajes y formas construidas.
Comercialización de las obras de arte. Investigación sobre la cerámica
Authors: Liu Jianxiong
Published: June 2022

Hoy en día, hay cada vez más personas que se dedican a la industria del arte en la sociedad. Gracias a esto constantemente surgen nuevas obras de arte y producciones. Por tantas opciones que existen, muchos clientes, creadores y productores suelen perderse fácilmente en esta deslumbrante variedad de obras de arte, especialmente los creadores de arte, quienes se encuentran en un dilema a la hora de comenzar a diseñar. Por lo tanto, cómo realizar mejor la comercialización de obras de arte se ha convertido en una cuestión importante para la mayoría de las personas que trabajan en esta área.

La cerámica es una técnica que se ha mantenido desde la antigüedad hasta la actualidad, y que da lugar a muchas producciones como distintas mercancías y obras de arte, por lo que la comercialización de obras de arte es considerada un buen objeto de estudio para el autor. Para sustentar mejor esta investigación, el autor analizará a dos ceramistas representativos de la revista internacional Cerámica, uno es Pablo Picasso y el otro, el ceramista contemporáneo Xavier Monsalvatje. A partir de su análisis, aprenderemos cómo crear mejores obras de arte y establecer una buena base para nuestro desarrollo comercial.

Por último, el autor también mostrará algunos de sus trabajos relacionados con este tema.

 

Palabras clave: comercialización de obras de arte, cerámica, Pablo Picasso, Xavier Monsalvaje, diseño.
Enseñar tintas antiguas en la educación superior de artes plásticas. Miradas pretéritas ante futuros inciertos.
Authors: Romero Pineda Rafael , Porquer Rigo Joan Miquel
Published: June 2022

El Grupo de Innovación Docente Consolidado ATESI (Arte, Territorio, Estrategia docente, Sostenibilidad e Intervención social – GINDOC-UB/162) de la Universidad de Barcelona plantea, entre otras, una línea de trabajo sobre materiales artísticos y praxis relacionadas en peligro de desaparición. Desde saberes de los oficios artísticos tradicionales se plantea la puesta en valor de elementos como las tintas antiguas y medievales, su uso actual en la educación superior de arte a través de diversas asignaturas y su expansión externa a la universidad. Este artículo presenta, precisamente, un ejemplo de implementación de metodología de enseñanza-aprendizaje de tintas tradicionales, una aproximación práctica a su fabricación y un ejemplo de sus resultados.
Fotografía infantil: análisis y métodos de enseñanza. Un estudio de caso basado en las artes visuales.
Authors: Moreno Córdoba Ángela
Published: June 2022

En este breve estudio introducimos el análisis de la fotografía espontánea infantil desde una metodología mixta, tanto cualitativa, a través de un estudio de caso, como basada en las artes visuales utilizando instrumentos de investigación basados en la fotografía. El objetivo principal de esta investigación es indagar sobre el aprendizaje de Clara (4 años) en sus procesos espontáneos de creación de imágenes fotográficas. A partir de estos análisis trataremos de elaborar una metodología de enseñanza de la fotografía. Partimos de dos preguntas de investigación: ¿Cuándo una fotografía infantil es espontánea? y ¿se puede establecer una relación entre la forma espontánea y aprendida de crear fotografías en edades tempranas? Para responder a estas preguntas hemos partido de una revisión exhaustiva de la literatura especializada más reciente para el diseño de un taller que permitiera tomar datos y analizarlos en profundidad, así como proponer diversas actividades de creación de imágenes que nos aportan datos de interés. En la discusión de datos se ha realizado un análisis de  las fotografías espontáneas de Clara, a través de series estudio, según son definidas por Roldán y Marín Viadel (2012). En dicho análisis hemos logrado identificar algunas de las claves características de la producción espontánea infantil partiendo de un esquema de cuatro fases que se desarrolló en un estudio previo (Moreno Córdoba, 2020): a) fotografía espontánea de toma de contacto; b) fotografía de experimentación; c) fotografía mejorada; y d) fotografía satisfactoria. Como conclusión a nuestro trabajo se presentan series y fotoensayos que muestran algunas coincidencias y divergencias entre los dos modelos de creación antes citados: espontáneo y aprendido.
Taller performativo a partir de acciones docentes, creadoras e investigadoras metodográficas
Authors: Caeiro Rodríguez Martín , Callejón Chinchilla María-Dolores , Vaquero Cañestro Carmen , Chacón Gordillo Pedro David
Published: June 2022

Presentamos un taller performativo en el que cuatro métodos creados de forma independiente por cada autor en su espacio académico van abriendo significados y posibilidades entre ellos provocadas por las sugerencias, afectos y respuestas que se van proponiendo de forma colectiva. El resultado no es un método universal y prescriptivo que encierra el acto de enseñar, aprender o investigar en las inercias de lo ya vivido, ya pensado, ya sentido, sino que es un método diverso, vivo, entreabierto y poroso que posibilita y adquiere nuevos sentidos en lo diálogos intersubjetivos docente-docente; docente-discente, discente-discente. Consideramos que los métodos en Educación Artística deben permitir recorridos metodográficos subjetivos que posibiliten lo poético sin dejar de “servir” a su propósito académico de investigar, enseñar, aprender arte.
La pandemia del coronavirus como oportunidad de innovación pedagógica. Investigación de Recursos Didácticos de Museos y Galerías de Arte
Authors: Jiroutová Jana , Mesdag Kateřina , Šobáňová Petra , Baštanová Pavla
Published: June 2022

Nuestro artículo trata sobre los recursos educativos en línea creados por los museos y galerías de arte de la República Checa en tiempos de la pandemia del coronavirus. Este artículo es el resultado parcial de un proyecto de investigación que actualmente llevan a cabo los autores en la Universidad Palacký de Olomouc en la República Checa. El objetivo principal de la investiga-ción es analizar los recursos educativos digitales accesibles en línea de los departamentos edu-cativos de los museos y galerías de arte, las formas de su presentación al público y la forma en que pueden ser utilizados por los educadores artísticos para la enseñanza a distancia de la edu-cación artística no solo en tiempos de coronavirus. Como parte de nuestra investigación, crea-remos un conjunto de estudios de casos, a partir de los cuales se formulará una tipología de recursos educativos en línea. Este documento presenta los resultados actualizados de la investi-gación, así como también analiza y brinda ejemplos de tres categorías de recursos educativos en línea, a saber, desafíos motivacionales, tutoriales educativos y sitios web interactivos.
Command, Control and Co-Creation: Drivers and Barriers Faced by Professionals Co-Creating in the Slovenian Public Sector
Authors: Vrbek Sanja , Kuiper Marlot
Published: June 2022

The academic contribution to the field is twofold. Firstly, the paper identifies co-creation drivers and barriers from the professionals’ point of view ‒ a largely overlooked perspective in the relevant literature. Secondly, by being placed in the Slovenian administrative context, the paper complements and enriches the debate on co-creation shaped predominantly by the experience of Northern and Western Europe.
Purpose: By focusing on the Slovenian public sector, as a traditionally hierarchical administrative context, the paper aims to identify factors that stimulate professionals to implement co-creation in their everyday work, as well as factors that impede their decision and behaviour in this direction.
Design/methodology/approach: The paper relies on two Slovenian case studies capturing the experience of professionals from the Ministry of Public Administration and the Municipality of Ljubljana. The key data-gathering methods were qualitative open-ended interviews with ‘lead professionals’ (at managerial positions) and focus groups with professionals who have regular contact with service users/external stakeholders and/or experience with co-creation.
Findings: The findings of the paper indicate that professionals implement co-creation even in ‘unfavourable’ hierarchical and centralised settings. A key driver for them to co-create in such a context is strong political support at the highest level. However, a more profound internalisation of co-creation depends on the redefinition of their professional identity, as well as on the (personal or collective/organisational) interest in co-creation.
Originality/significance: The paper makes a pioneer step in providing an in-depth look into the drivers and barriers professionals face when implementing co-creation in the Slovenian public sector. As such, it provides valuable input for further comparative analyses of co-creation drivers and barriers faced by professionals across Central and Eastern Europe.
Local Policies in the Fight Against the Covid-19 Pandemic: Ankara and Rome Municipal Councils’ Decisions
Authors: Hamza Çelikyay Hicran , Küçük Bayraktar Hülya
Published: June 2022

Purpose and methodology: This study examines how the COVID-19 pandemic is included in municipal councils’ decisions and investigates the local policies developed through these decisions. Ankara and Rome Municipalities were selected as study samples. Turkey and Italy are both unitary states and their local government structures are similar because their local government systems are part of the Franco group, as defined by Hesse and Sharpe. Both cities have gained the status of metropolitan cities under the legal regulations adopted in recent years. For this reason, Ankara and Rome present similarities in terms of both the areas they serve and the responsibilities of being the capital. The decisions of their municipal councils were reviewed from March to December 2020. The decisions were analysed within the framework of the relevant commissions and application areas, and the local policies implemented in the fight against COVID-19 were put forward.
Findings: According to the analyses, the Ankara Metropolitan Municipal Council intensified its decisions on economic support, social assistance, cleaning, public health, and local diplomacy, while the Rome Metropolitan Municipality’s policies focused on security, education, economic support, social assistance, cleaning, and transportation services. As a result, both municipalities brought their COVID-19 proposals to the agenda of the council. Municipal assemblies played a vital role in helping their countries fight the pandemic as administrative units that provide the legal basis for implementing local policies. Strengthening local assemblies and expanding their mandate and responsibility in times of crisis could also support the success of central government policies. The findings reveal that fast and effective solutions delivered by local governments through local policies successfully curbed the pandemic that had spread around the world.
Practical Implications: The policies implemented by local governments were impressive and complementary to central government policies, providing a valuable guideline for policymakers.
Water Quality and Microbial Community Assessment in Artisanal Mining- Affected Sediments of Cikidang River, Banten, Java, Indonesia
Authors: Takarina Noverita Dian , Sukma Ayu Maulida , Adiwibowo Andrio , Sitaresmi Sitaresmi
Published: June 2022

The total microbial diversity and community in submerged sediments near mining sites, transition and intact sites at Cikidang River, Banten were assessed using 16S rDNA sequence data and biodiversity indices. Assessed water quality parameters in the river were water current, dissolved oxygen (DO), pH, redox potential, salinity, temperature and turbidity. Microbial alpha diversity used were Shannon diversity index, ChaoI index and Operational Taxonomic Units (OTUs). These analyses indicated a total of 50 taxa of sediment microbes. Based on Shannon diversity index, the highest value was observed at mining site. High abundant microbes in sediments at mining sites for phylum, class, order, family and genera levels were represented by Proteobacteria, Gammaproteobacteria, Pseudomonadales, Moraxellaceae, and Acinetobacter, respectively. In contrast, high abundant microbes in the sediment of the intact site for each taxon consisted of Firmicutes, Alphaproteobacteria, Erysipelotrichales, Erysipelotrichaceae, and Erysipelothrix. The water quality of mining sites was characterised by alkaline pH (8.807, 95% CI: 8.624 - 8.990) and lower redox potential (59.000 mV, 95% CI: 36.233 - 81.767) that differed significantly from the intact sites (P<0.05). This study also confirmed that mining sites have a higher genus diversity. Research on potential microbes of related genus as bioremediators could be recommended for further study.
Epidermal Mucus of Anabas testudineus as a Promising Source of Antibacterial and Anticancer Agents
Authors: Najm Ahmed Abdulkareem , Eziwar Dyari Herryawan Ryadi , Othman Babul Airianah , Syed Alwi Sharifah Sakinah , Azfaralarriff Ahmad , Shahid Muhammad , Sanusi Siti Aisyah , Law Douglas , Fazry Shazrul
Published: June 2022

Anabas testudineus is a sturdy freshwater fish that can live in a polluted environment due to the epidermal mucus (EM) that protects the fish from pathogens or germs. This study explored the functional properties of the EM as a potential antimicrobial and anticancer agent. Inactive Pseudomonas aeruginosa was introduced into fish tanks to stimulate the production of EM. This stimulus significantly increased EM production by more than 100% after 10 days of stimulation, indicating that EM production was influenced by environmental biotic stress. In vitro antibacterial activity tests showed that EM has significant antibacterial activity against Escherichia coli (12 ± 0.23 mm) and P. aeruginosa (10 ± 0.13 mm) at the tested concentration of 1000 μg/mL. Further characterisation against cells showed that EM has a cytotoxic effect against human breast cancer (MCF7) and human melanoma (A375.S2) producing an IC50 value of 4.97 ± 0.25 and 6.27 ± 0.17 mg/mL, respectively. In contrast, no cytotoxicity against normal fibroblast skin cells (HS27) was observed. In addition, apoptosis analysis showed that EM could cause DNA fragmentation of cancer cells, while no effect on normal cells was observed. These findings indicated that EM from A. testudineus could be further studied and explored as an anticancer agent.
Characterization of Keratinocytes, Fibroblasts and Melanocytes Isolated from Human Skin using Gene Markers
Authors: Wong Pau-Ring , Mohd Sahardi Nur Fatin Nabilah , Tan Jen-Kit , Chua Kien-Hui , Wan Ngah Wan Zurinah , Makpol Suzana
Published: June 2022

Cells isolated from skin have wide applications in studies of the pathogenesis of skin-related diseases and the construction of 3D skin equivalents. This study aimed to isolate keratinocytes, fibroblasts, and melanocytes from human foreskin and characterize the purity of the cell types. Keratinocytes, fibroblasts, and melanocytes from human foreskin were isolated by differential trypsinization and media selection. The purity of the cell types was characterized based on the expression of gene markers. The assessment of gene marker expression involved RNA extraction, primer design, quantitative polymerase chain reaction (qPCR) and immunocytochemical staining. Our results showed that in cocultures of keratinocytes and fibroblasts isolated from the dermis, fibroblasts could be separated from keratinocytes by quick trypsinization and culture in Dulbecco’s modified Eagle’s medium. The remaining keratinocytes are cultured in Epilife medium. Melanocytes in cocultures of melanocytes and keratinocytes isolated from the epidermis could be selected by changing Epilife medium to M254 medium. Gene marker results suggested that cytokeratin 14 (CK14) is a suitable marker for keratinocytes, elastin (ELN) is a suitable marker for fibroblasts, and tyrosinase (TYR) and tyrosinase-related proteins 1 and 2 (TYRP1 and TYRP2) are suitable markers for melanocytes. In conclusion, keratinocytes, fibroblasts, and melanocytes can be isolated from the same human foreskin sample by differential trypsinization and media selection techniques and characterized by suitable gene markers. This finding will aid in the isolation of pure skin cell types for various applications in regenerative medicine and toxicity studies.
In Vitro Anti-Angiogenic Properties of Ethanolic Crude Extract of Vernonia amygdalina
Authors: Al-deeb Ibrahim , Joseph Julia , Abdul Majid Amin Malik Shah , Abdul Majid Aman Shah , Abdul Samad Nozlena
Published: June 2022

Angiogenesis is the process of generating new blood vessels that deliver tumor cells with oxygen and essential nutrients for growth and metastasis. This study examined the in vitro antiangiogenic properties of the ethanolic crude extract from Vernonia amygdalina (VA) grown in Malaysia. The direct antiangiogenic activity of VA was evaluated on EA.hy926 cells using in vitro assessments: Cell proliferation, colony formation, migration, and cell invasion assays. VA ethanolic crude extract cytotoxic activity was evident in the antiproliferative and colony formation assays. The growth inhibition (IC50) of 50% against EA.hy926 endothelial cells was achieved after 72 h treatment at a concentration of 85.43±3.57 µg/mL. Upon 48 h treatment, colony formation was inhibited completely at 100 µg/mL while 51.94% inhibition was achieved at 50 µg/mL. Moreover, the extract showed 54.72% and 31.99% inhibitory effects against migration of cells when treated for 24 h treatment at two different concentrations, 25 µg/mL and 12.5 µg/mL, respectively. The use of 100 µg/mL VA ethanolic extract inhibited cell invasion by 35.43%, which was lower than that of 57.81% inhibition achieved by the vinblastine as a positive control. All in all, the present work clearly demonstrated the antiangiogenic properties of VA ethanolic extract that may reflect a chemotherapeutic and/or chemoprevention potential for biomedical applications.
Insights into Variations in COVID-19 Infections in Malaysia
Authors: Sanmugan Kirubha A/P , Abdul Talib Muzalwana
Published: June 2022

The present study aims to visualize the variations in the number of confirmed daily COVID-19 infections during the third wave in Malaysia through the application of control charts. This study also attempts to propose the target number of daily new cases that would bring the pandemic situation in Malaysia under control by utilizing the confirmed daily cases in Malaysia starting from 8th September 2020 until 30th June 2021. A modified Shewhart control chart was adopted to monitor the variations before and after the commencement of National Immunisation Programme (NIP). The chart shows a declining trend in the number of cases after the rollout of NIP whereby several days were brought to a state of statistical-in-control. But in less than three months after NIP commencement, there were huge variations in COVID-19 cases leading to drastic increase in the mean number of cases. These signal the presence of unnatural or assignable causes of variations which could be attributed to failure of curbing the risks of transmission, existence of various variants in the community, easing of containment measures and less adherence to the COVID-19 Standard Operating Procedures (SOPs). Significant shifts in the mean values prompt the development of a 3-phase modified Shewhart control chart. From the 3-phase chart, a series of daily number new cases that can be set as the target value to bring the pandemic situation in Malaysia under control, while flattening the epidemiological curve in the very near term.
The Incipient Neolithic of the Kurile Islands: The Culture of Long Barrows
Authors:   Sakhalin State University, Grishchenko V. A. , Pashentsev P. A. ,   OOO “Izyskatel SakhGU”, Yuzhno-Sakhalinsk, Russia, Vasilevski А. А. ,   Sakhalin State University
Published: June 2022

Статья посвящена выделению новой неолитической культуры островного мира северо-востока Азии и обоснованию начального этапа неолита Курильских островов на основе материалов поселений Китовый-2 и -4 (о-в Итуруп, Большая Курильская гряда, Сахалинская обл.), исследованных в 2019-2020 гг. Выявлено несколько типов неолитических объектов, впервые зафиксированных на Курильских островах в рамках раскопок широкой площадью. Основой обнаруженного комплекса являются два типа жилищ — наземное без заглубления деревянного каркаса и полуземлянка с линейно-рельефной керамикой и каменной индустрией, ориентированной на бифасиальные технологии изготовления ретушированных орудий из отщепов и целых отдельностей, с развитой шлифовкой камня. Также впервые на Курильских островах выявлены площадки с длинными насыпями и ограждением из выложенных по периметру базальтовых плиток. Таким образом, в ходе раскопок археологическими методами зафиксировано ритуальное поведение, выразившееся в устройстве огражденных плиточных кладок неутилитарного назначения — предположительно мест погребения кремированных останков умерших и отправления культа предков. На основании данного весьма важного признака предлагается объединить изученные неолитические комплексы поселений Китовый-2 и -4 в рамках культуры длинных могил. Полученные стратиграфические данные, подкрепленные радиоуглеродным датированием объектов, позволяют выделить начальный этап эпохи неолита Курильских островов в хронологических рамках 13,0-8,5 тыс. кал. л.н.
An Early Neolithic Sanctuary in the Eastern Irtysh Basin
Authors:   Institute of Archaeology and Ethnography, Siberian Branch, Russian Academy of Sciences, Molodin V. I. ,   Novosibirsk State University, Mylnikova L. N. ,   Institute of Archaeology and Ethnography, Siberian Branch, Russian Academy of Sciences,   Novosibirsk State University, Nesterova M. S. ,   Institute of Archaeology and Ethnography, Siberian Branch, Russian Academy of Sciences,   Novosibirsk State University, Kobeleva L. S. ,   Institute of Archaeology and Ethnography, Siberian Branch, Russian Academy of Sciences,   Novosibirsk State University, Selin D. V. ,   Institute of Archaeology and Ethnography, Siberian Branch, Russian Academy of Sciences,   Novosibirsk State University
Published: June 2022

Публикуются результаты комплексного исследования уникального святилища в Барабинской лесостепи, которое представляет собой своеобразный архитектурный ансамбль, состоящий из ровика, оконтуривающего сакральное пространство, и системы ям с разнообразными прикладами культового характера. Наличие углублений на дне ровика позволяет предполагать, что изначально конструкцию дополняли деревянные сооружения. В статье дается описание особенностей объектов. Охарактеризован вещественный комплекс, содержащий изделия хозяйственно-бытового, производственного и ритуального назначения. На основе стратиграфических наблюдений и серии радиоуглеродных дат сделан вывод об относительной и абсолютной хронологии комплекса. Объект датирован ранненеолитическим временем (в пределах VII-VI тыс. до н.э.) и отнесен к барабинской культуре. Проведены параллели с мезолитическими и неолитическими святилищами и культовыми комплексами, которые соответствуют таежной зоне Евразии.
Bioarchaeology of Childhood in the Yamnaya Culture, Based on Kurgan 1 at Boldyrevo-4, the Southern Urals
Authors:   Orenburg State Pedagogical University, Morgunova N. L. , Faizullin A. A. ,   Orenburg State Pedagogical University, Chechyotkina O. Y. ,   Institute of Archaeology, Russian Academy of Sciences, Mednikova M. B. ,   Institute of Archaeology, Russian Academy of Sciences
Published: June 2022

Рассмотрены археологические и палеоантропологические данные о двух детских погребениях, представляющих ранний горизонт в кург. 1 могильника Болдырево-4 в Оренбургской обл., который был исследован в 2019-2020 гг. Ранние насыпи перекрывались огромным курганом над другим, более поздним захоронением взрослых людей. Весь комплекс был сооружен носителями ямной культуры на рубеже раннего и развитого ее этапов, ок. 3300-3100 кал. лет до н.э. Изучены останки трех детей в возрасте ок. 6 лет из двух могил. Выявлены следы серьезных палеопатологий. Ребенок из погр. 3 скончался от метастатического рака. Ребенок 1 из погр. 4, останки которого представлены одним черепом, очевидно, страдал от цинги. Возможными причинами онкологического заболевания могли стать долгое пребывание у задымленного очага или близость к месту металлообработки, поскольку носители ямной культуры Южного Урала активно разрабатывали медные месторождения. В обоих детских комплексах выявлены как стандартные для ямного погребального обряда признаки, так и некоторые особенности. Установлено, что сосуды из погребений близки по форме и орнаментации, но отличаются по технологии изготовления. Различен состав органических подстилок под скелетами. Отличия могут свидетельствовать о принадлежности погребенных к родственным, но самостоятельным родовым коллективам. Очевидно, что дети, захороненные под ранними насыпями, имели наследственный социальный статус, который сыграл роль в дальнейшем сооружении элитного кургана.
A Functional Analysis of Lithics of the Early Iron Age Yankovsky Culture: New Findings
Authors:   Far Eastern Federal University, Popov A. N. , Kanomata Yoshitaka ,   Tohoku University, Rudenko M. K. ,   Far Eastern Federal University, Lazin B. V. ,   Far Eastern Federal University
Published: June 2022

В статье представлены результаты функционального анализа каменных орудий янковской культуры (VIII-I вв. до н.э.) с поселений Черепаха-7 и Солонцовая-2. Памятники раскопаны большими площадями в ходе спасательных работ в 2015 и 2017 г. соответственно. Рассматриваются орудия, традиционно определяемые по морфологическим признакам как топоры, тесла, долота, ножи, наконечники копий и дротиков, жатвенные ножи. Представлены новые данные о функциях орудий янковской культуры, не согласующиеся с принятой классификацией. Поднимается вопрос о соответствии формально-типологических и трасологических определений. Функциональный анализ проводился с использованием т.н. метода Кили (High Power Approach), а также классификации типов заполировки, разработанной специалистами из Университета Тохоку (Япония). Определены функции 28 из 62 орудий, отобранных для анализа. Уточнена существующая номенклатура типов орудий деревообработки, существенно конкретизирована информация о технике уборки травянистых растений, орудийном наборе кожевенного дела, приведены сведения об орудиях, использовавшихся в косторезном деле, а также применявшихся для открывания раковин двустворчатых моллюсков. Высокоточный функциональный анализ каменного инвентаря янковской культуры позволяет максимально углубить понимание назначения каменных орудий, которые, несмотря на знакомство носителей этой культуры с металлами, составляли основу ее технологической базы. Намечен ряд возможных направлений для дальнейших трасологических исследований.
Analysis and Museumization of a Wooden Burial Structure from Pazyryk Kurgan 5, the Altai Mountains: A Methodological Study
Authors:   Institute of Archaeology and Ethnography, Siberian Branch, Russian Academy of Sciences, Mylnikov V. P.
Published: June 2022

В статье представлены обоснование и опыт практического применения комплекса методов исследования, сохранения и ввода в экспозицию музея в течение одного полевого сезона погребального сооружения из дерева раннего железного века. Публикуются результаты междисциплинарного изучения двухсрубной конструкции с надсрубными постройками в могильной яме кург. № 5 памятника Пазырык, а также технико-технологического исследования всех составляющих погребального устройства в процессе раскопок и камеральной обработки после извлечения его из могилы. Приводится информация о проведении рядом с курганом экспресс-реконструкции внешнего сруба по отработанной ранее методике, дается описание его конструктивных и технологических особенностей. В описании хода работ и методов изучения погребальных сооружений указаны этапы полевой консервации всех обнаруженных артефактов. Показан процесс музеефикации внешнего сруба в Национальном музее Республики Алтай им. А.В. Анохина.
Rocks in the Religious Beliefs and Rites of the Ob Ugrians
Authors:   Institute of Archaeology and Ethnography, Siberian Branch, Russian Academy of Sciences, Baulo A. V.
Published: June 2022

В работе, основанной на материалах, которые были собраны исследователями в XVIII-XX вв., а также результатах экспедиций автора за последние 40 лет, показано место камней в мировоззрении и религиозно-обрядовой практике обских угров. Приведены сведения о почитании различных природных объектов из камня, раскрыты причины поклонения подобным культовым местам. Установлено, что почитались природные объекты, имевшие антропоморфные или зооморфные очертания. Каменные дома богатырей-предков в легендах и мифах соотнесены с многочисленными пещерами в горах Урала. В основной части статьи представлены материалы о каменных фигурах божеств и духов-покровителей; они подкреплены мифическими сюжетами, объясняющими способы обращения героев в камень. Даются примеры изготовления фигур божеств с основой в виде камня у локальных групп обских угров, в частности, ляпинских и сосьвинских манси, сынских и полуйских хантов. Рассмотрена роль камней в родильном и погребальном обрядах, хозяйственной деятельности, их значение в различного рода магических церемониях при отправлении хозяйственной деятельности, в частности, рыболовства. Отмечена равнозначность понятий «каменный», «железный», «сильный» в мифологических представлениях хантов и манси.
Paleogenetic Studies of Migration Processes in Eurasia
Authors:   Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Pilipenko A. S. , Trapezov R. O. ,   Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Cherdantsev S. V. ,   Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
Published: June 2022

Миграционные процессы играли ключевую роль в формировании культурного и генетического ландшафта Евразии. Значительный прогресс, достигнутый в области изучения миграций в последние годы, связан с развитием методов исследования древней ДНК. Они позволяют выйти на принципиально новый уровень понимания популяционно-генетических аспектов древних миграционных процессов, существенно дополняя возможности физической палеоантропологии и генетики современных популяций, но не заменяя собой эти направления. Актуальной остается проблема корректного сопоставления процессов, сопровождавших миграции на уровне генетического состава населения и его материальной культуры. В статье освещается современный уровень методов исследования структуры древней ДНК: от классического анализа отдельных генетических маркеров до полногеномного с помощью высокопроизводительного секвенирования. Рассматриваются подходы к изучению древних миграций, объективной реконструкции генетического состава населения и его динамики во времени и пространстве. Особое внимание уделено проблеме репрезентативности популяционных выборок при исследовании миграционных процессов методами палеогенетики, возможным стратегиям выбора материала, наиболее адекватного поставленным задачам. Дано обоснование наибольшей эффективности диахронного подхода при реконструкции генетической истории популяций. Обсуждаются возможные перспективы развития направления, включая переход к более детализированным локально-территориальным реконструкциям миграционных процессов.