bluuebunny/crossref_metadata_2025 huggingface dataset:
Prediction of Drug Synergism between Peptides and Antineoplastic Drugs Paclitaxel, 5-Fluorouracil, and Doxorubicin Using In Silico Approaches
Authors: Vale Nuno , Pereira Mariana , Santos Joana , Moura Catarina , Marques Lara , Duarte Diana
Published: December 2022

Chemotherapy is the main treatment for most early-stage cancers; nevertheless, its efficacy is usually limited by drug resistance, toxicity, and tumor heterogeneity. Cell-penetrating peptides (CPPs) are small peptide sequences that can be used to increase the delivery rate of chemotherapeutic drugs to the tumor site, therefore contributing to overcoming these problems and enhancing the efficacy of chemotherapy. The drug combination is another promising strategy to overcome the aforementioned problems since the combined drugs can synergize through interconnected biological processes and target different pathways simultaneously. Here, we hypothesized that different peptides (P1–P4) could be used to enhance the delivery of chemotherapeutic agents into three different cancer cells (HT-29, MCF-7, and PC-3). In silico studies were performed to simulate the pharmacokinetic (PK) parameters of each peptide and antineoplastic agent to help predict synergistic interactions in vitro. These simulations predicted peptides P2–P4 to have higher bioavailability and lower Tmax, as well as the chemotherapeutic agent 5-fluorouracil (5-FU) to have enhanced permeability properties over other antineoplastic agents, with P3 having prominent accumulation in the colon. In vitro studies were then performed to evaluate the combination of each peptide with the chemotherapeutic agents as well as to assess the nature of drug interactions through the quantification of the Combination Index (CI). Our findings in MCF-7 and PC-3 cancer cells demonstrated that the combination of these peptides with paclitaxel (PTX) and doxorubicin (DOXO), respectively, is not advantageous over a single treatment with the chemotherapeutic agent. In the case of HT-29 colorectal cancer cells, the combination of P2–P4 with 5-FU resulted in synergistic cytotoxic effects, as predicted by the in silico simulations. Taken together, these findings demonstrate that these CPP6-conjugates can be used as adjuvant agents to increase the delivery of 5-FU into HT-29 colorectal cancer cells. Moreover, these results support the use of in silico approaches for the prediction of the interaction between drugs in combination therapy for cancer.
Dysferlin Deficiency Results in Myofiber-Type Specific Differences in Abundances of Calcium-Handling and Glycogen Metabolism Proteins
Authors: Lloyd Erin M. , Pinniger Gavin J. , Grounds Miranda D. , Murphy Robyn M.
Published: December 2022

Dysferlinopathies are a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults. The disease is characterized by progressive skeletal muscle wasting in the limb-girdle and limbs, inflammation, accumulation of lipid droplets in slow-twitch myofibers and, in later stages, replacement of muscles by adipose tissue. Previously we reported myofiber-type specific differences in muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice that could not be fully accounted for by altered myofiber-type composition. In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice. Additionally, we compared the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice. Differences were observed for some Ca2+ handling and glucose/glycogen metabolism-related protein levels between BLAJ soleus and EDL muscles (compared with WT) that may contribute to the previously reported differences in function in these BLAJ muscles. Dysferlin deficiency did not impact glycogen content of whole muscles nor Ca2+ activation of the myofilaments, although soleus muscle from 10-month-old BLAJ mice had more glycogen than EDL muscles. These results demonstrate a further impact of dysferlin deficiency on proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles, potentially contributing to altered contractile function in dysferlinopathy.
Roles and Mechanisms of Long Non-Coding RNAs in Breast Cancer
Authors: Su Jia , Deng Lihao , Wang Yan-Dong
Published: December 2022

Breast cancer is a major health threat and the second leading cause of cancer-related deaths in women worldwide. The detailed mechanisms involved in the initiation and progression of breast cancer remain unclear. In recent years, amounting evidence indicated that long non-coding RNAs (lncRNAs) played crucial roles in regulating various biological processes and malignancy tumors, including breast cancer. In this review, we briefly introduce the functions and underlying mechanisms by which lncRNAs are involved in breast cancer. We summarize the roles of the lncRNAs in regulating malignant behaviors of breast cancer, such as cell proliferation, migration and invasion, epithelial–mesenchymal transition (EMT), apoptosis, and drug resistance. Additionally, we also briefly summarize the roles of circular RNAs (circRNAs) in breast cancer carcinogenesis.
Long-Term Refrigerated Storage of Beef Using an Active Edible Film Reinforced with Mesoporous Silica Nanoparticles Containing Oregano Essential Oil (Lippia graveolens Kunth)
Authors: Matadamas-Ortiz Alexis , Hernández-Hernández Elvia , Castaño-Tostado Eduardo , Amaro-Reyes Aldo , García-Almendárez Blanca E. , Velazquez Gonzalo , Regalado-González Carlos
Published: December 2022

Beef is a fundamental part of the human diet, but it is highly susceptible to microbiological and physicochemical deterioration which decrease its shelf life. This work aimed to formulate an active edible film (AEF) incorporated with amino-functionalized mesoporous silica nanoparticles (A-MSN) loaded with Mexican oregano (Lippia graveolens Kunth) essential oil (OEO) and to evaluate its effect as a coating on fresh beef quality during refrigerated storage. The AEF was based on amaranth protein isolate (API) and chitosan (CH) (4:1, w/w), to which OEO emulsified or encapsulated in A-MSN was added. The tensile strength (36.91 ± 1.37 MPa), Young’s modulus (1354.80 ± 64.6 MPa), and elongation (4.71%) parameters of AEF made it comparable with synthetic films. The antimicrobial activity of AEF against E. coli O157:H7 was improved by adding 9% (w/w) encapsulated OEO, and interactions of glycerol and A-MSN with the polymeric matrix were observed by FT-IR spectroscopy. In fresh beef, after 42 days, AEF reduced the population growth (Log CFU/cm2, relative to uncoated fresh beef) of Brochothrix thermosphacta (5.5), Escherichia coli (3.5), Pseudomonas spp. (2.8), and aerobic mesophilic bacteria (6.8). After 21 days, odor acceptability of coated fresh beef was improved, thus, enlarging the shelf life of the beef and demonstrating the preservation capacity of this film.
Sex and HDAC4 Differently Affect the Pathophysiology of Amyotrophic Lateral Sclerosis in SOD1-G93A Mice
Authors: Renzini Alessandra , Pigna Eva , Rocchi Marco , Cedola Alessia , Gigli Giuseppe , Moresi Viviana , Coletti Dario
Published: December 2022

Amyotrophic Lateral Sclerosis (ALS) is a devastating adult-onset neurodegenerative disease, with ineffective therapeutic options. ALS incidence and prevalence depend on the sex of the patient. Histone deacetylase 4 (HDAC4) expression in skeletal muscle directly correlates with the progression of ALS, pointing to the use of HDAC4 inhibitors for its treatment. Contrarily, we have found that deletion of HDAC4 in skeletal muscle worsened the pathological features of ALS, accelerating and exacerbating skeletal muscle loss and negatively affecting muscle innervations in male SOD1-G93A (SOD1) mice. In the present work, we compared SOD1 mice of both sexes with the aim to characterize ALS onset and progression as a function of sex differences. We found a global sex-dependent effects on disease onset and mouse lifespan. We further investigated the role of HDAC4 in SOD1 females with a genetic approach, and discovered morpho-functional effects on skeletal muscle, even in the early phase of the diseases. The deletion of HDAC4 decreased muscle function and exacerbated muscle atrophy in SOD1 females, and had an even more dramatic effect in males. Therefore, the two sexes must be considered separately when studying ALS.
Quantitative Metabolomics to Explore the Role of Plasma Polyamines in Colorectal Cancer
Authors: Coradduzza Donatella , Arru Caterina , Culeddu Nicola , Congiargiu Antonella , Azara Emanuela Gigliola , Scanu Antonio Mario , Zinellu Angelo , Muroni Maria Rosaria , Rallo Vincenzo , Medici Serenella , Carru Ciriaco , Angius Andrea , De Miglio Maria Rosaria
Published: December 2022

Colorectal cancer (CRC) is one of the major public health and socio-economic problems, which management demands the development of non-invasive screening tests. Assessment of circulating polyamines could be a valuable tool, although analytical problems still preclude its clinical practice. We exploited ultra-high-resolution liquid chromatography and mass spectrometry, as a highly sensitive and innovative method, to profile eleven polyamines, including spermine and spermidine with their acetylated forms. These data together with an evaluation of the inflammatory indexes might represent suitable biomarkers for the identification of CRC patients. The statistical models revealed good discrimination in distinguishing CRC patients from healthy subjects. The plasma assessment of ornithine and acetylspermine, as well as lymphocyte/platelet ratio, revealed helpful information on the progression of CRC. The combined profiles of circulating polyamines and inflammatory indexes, together with the application of an innovative technology, could represent a valuable tool for discriminating patients from different clinical groups.
Timing of ICSI with Respect to Meiotic Spindle Status
Authors: Tepla Olga , Topurko Zinovij , Jirsova Simona , Moosova Martina , Fajmonova Eva , Cabela Radek , Komrskova Katerina , Kratochvilova Irena , Masata Jaromir
Published: December 2022

The aim of this study was to evaluate the efficiency of using meiotic spindle (MS) visibility and relative position to the polar body (PB) as indicators of oocyte maturation in order to optimize intracytoplasmic sperm injection (ICSI) timing. This was a cohort study of patients younger than 40 years with planned ICSI, the timing of which was determined by MS status, compared with those without MS evaluation. The angle between PB and MS and MS visibility were evaluated by optical microscope with polarizing filter. Oocytes with MS evaluation were fertilized according to MS status either 5–6 h after ovum pick-up (OPU) or 7–8 h after OPU. Oocytes without MS evaluation were all fertilized 5–6 h after OPU. For patients over 35 years visualization of MS influenced pregnancy rate (PR): 182 patients with MS visualization had 32% PR (58/182); while 195 patients without MS visualization had 24% PR (47/195). For patients under 35 years, visualization of MS did not influence PR: 140 patients with MS visualization had 41% PR (58/140), while 162 patients without MS visualization had 41% PR (66/162). Visualization of MS therefore appears to be a useful parameter for assessment of oocyte maturity and ICSI timing for patients older than 35.
Copper Complexes of Silicon Pyridine-2-olates RSi(pyO)3 (R = Me, Ph, Bn, Allyl) and Ph2Si(pyO)2
Authors: Seidel Anne , Gericke Robert , Brendler Erica , Wagler Jörg
Published: December 2022

The organosilicon pyridine-2-olates 1a–1d (RSi(pyO)3, R = Me (a), Ph (b), Bn (c), Allyl (d); pyO = pyridine-2-olate) may serve as tripodal ligands toward CuCl with formation of complexes of the type RSi(μ2-pyO)3CuCl (2a–2d). In addition, for R = Allyl, formation of the more stable isomer 2d′ (κO-pyO)Si(μ2-pyO)2(μ2-Allyl)CuCl was observed. In the presence of dry air (as a source of oxygen), reactions of 1a–1d and CuCl afforded Cu(II) complexes RSi(μ2-pyO)4CuCl (3a–3d); 3a–3c in good yield, and 3d only as a side product. Reaction of Ph2Si(pyO)2 (4) and CuCl in equimolar ratio afforded, depending on reaction conditions, a series of (CuCl)n-ladder-type oligonuclear Cu(I) complexes Ph2Si(μ2-pyO)2(CuCl)n(μ2-pyO)2SiPh2 (n = 2 (52), 3 (53), 4 (54)). In all of the above compounds, the pyO group is Si–O bound and, in the case of μ2 coordination, Cu–N bound. All new compounds (1c, 1d, 2b, 2c, 2d, 2d′, 3b, 3c, 3d, 52, 53, 54) were characterized by single-crystal X-ray diffraction, and further characterization includes solution 1H, 13C, 29Si NMR spectroscopy (1c, 1d, 2b, 2c, 2d’, 53, 54), solid-state 29Si (2b, 2c, 2d′, 53, 54) and 63Cu NMR spectroscopy (2c, 2d′) as well as computational analyses of the isomerization of the couple 2d, 2d′.
Tetramethylcyclopentadienyl Samarium(II) Metallocene Chemistry: Isolation of a Bimetallic Sm(II)/Sm(II) Complex
Authors: Nguyen Joseph Q. , Ziller Joseph W. , Evans William J.
Published: December 2022

The salt metathesis reaction between one equivalent of SmI2(THF)2 and two equivalents of K(C5Me4H) in THF afforded single crystals of the unusual, toluene-soluble, and asymmetric bimetallic Sm(II)/Sm(II) complex, (C5Me4H)2SmII(μ-η3:η5-C5Me4H)SmII(C5Me4H)(THF)2, instead of the expected product, (C5Me4H)2SmII(THF)2. The toluene-insoluble products of this reaction can be worked up in 1,2-dimethoxyethane (DME) to provide X-ray quality crystals of the monomeric Sm(II) metallocene, (C5Me4H)2SmII(DME). (C5Me4H)2SmII(DME) can also be synthesized directly by the reaction between one equivalent of SmI2(THF)2 and two equivalents of K(C5Me4H) in neat DME. The isolation and characterization of the bimetallic Sm(II)/Sm(II) complex provides supporting evidence for the possible oligomerization that may occur during the synthesis of Sm(II) complexes with cyclopentadienyl ligands that are less sterically bulky and less solubilizing than (C5Me5)1−.
Interspecific Competitions between Frankliniella intonsa and Frankliniella occidentalis on Fresh Lentil Bean Pods and Pepper Plants
Authors: Yang Chun-Hong , Qiao Feng-Jiao , Lu Zhaozhi , Li Chang-You , Liu Tong-Xian , Gao Yu-Lin , Zhang Bin
Published: December 2022

Background: Flower thrips (Frankliniella intonsa, IFT) and west flower thrips (Frankliniella occidentalis, WFT) are often found together on the host plant in China. WFT is an important invasive species that seems to outcompete the native IFT. In order to clarify the interspecific competitions between the two thrips, this study measured the population development of IFT and WFT under sexual and parthenogenetic reproductive modes on two hosts (fresh lentil bean pods with/without honey and pepper plants at seedling/flowering stages) in the laboratory. Results: When reared on fresh lentil bean pods (with/without honey), WFT population size was lower in mixed species populations compared to single species populations but the presence of WFT had nor negative effect on IFT population size. These results were dependent of the reproductive mode. When honey was supplied, the ratio of female-to-male in the progeny of WFT produced under sexual reproductive mode increased significantly in the presence of IFT. On pepper seedlings, mixed populations were more favorable to the population development of IFT at the seedling stage, but more favorable to WFT at the flowering stage. Conclusions: In the early stage of WFT invasion and colonization, the emergence of flowering and honey (nectar) sources may have a positive effect on the population development of WFT.
Intra-Articular Calcaneal Fractures: Comparison between Mini-Invasive Approach and Kirschner Wires vs. Extensive Approach and Dedicated Plate—A Retrospective Evaluation at Long-Term Follow-Up
Authors: Caravelli Silvio , Gardini Giammarco , Pungetti Camilla , Gentile Paolo , Perisano Carlo , Greco Tommaso , Rinaldi Vito , Marcheggiani Muccioli Giulio , Tigani Domenico , Mosca Massimiliano
Published: December 2022

Introduction: Calcaneal fractures (CF) are the most common tarsal fractures, representing up to 75% of foot fractures and 2% of all fractures. The aim of this retrospective study is to analyze fixation with Kirschner wires through a mini-invasive approach and dedicated plate and screws through an extended approach at long-term follow-up. Materials and Methods: Patients were radiographically and clinically evaluated at final follow-up, by using the validated American Orthopedic Foot and Ankle Society (AOFAS) Ankle-Hindfoot score for the clinical–functional assessment, the Short-Form 12 (SF-12) for the physical and psychological domain assessment, and the Visual Analog Scale (VAS) for pain. Results: In total, 38 patients (42 CF) met the inclusion criteria and were retrospectively evaluated and divided into two groups (Kirschner group and plate group) consisting of 19 patients each. The overall mean follow-up was 59.4 ± 11.8 months. The average values of the post-operative clinical outcomes of the two groups KG and PG were, respectively, 70.7 ± 11.9 and 70.1 ± 10.9 (AOFAS), 45.7 ± 6.8 and 46.5 ± 10.8 (SF-12 PCS), 54.7 ± 9.9 and 50.9 ± 11.8 (SF-12 MCS) at the final follow-up. Conclusions: The present study showed that in the cases analyzed, the two surgical approaches used for the treatment of CF achieved comparable clinical outcomes. The only substantial difference found between the two groups of patients was the re-intervention rate that afflicted them.
Patient Selection for Pemafibrate Therapy to Prevent Adverse Cardiovascular Events
Authors: Izumida Toshihide , Imamura Teruhiko , Narang Nikhil , Kinugawa Koichiro
Published: December 2022

Background: pemafibrate is a newly-introduced selective peroxisome proliferator-activated receptor-α modulator, which decreases serum triglyceride levels with few drug-related adverse events and may reduce the risk of adverse cardiovascular events in carefully selected patients with hypertriglyceridemia. We aimed to understand which specific cohorts may benefit or not from pemafibrate therapy for adverse cardiovascular event risk reduction. Methods: patients with hypertriglyceridemia at baseline received pemafibrate therapy for two years or until October 2022. The factors that were associated with an increased risk of adverse cardiovascular events, defined as heart failure hospitalization, stroke, and acute coronary syndromes, were investigated. Results: a total of 121 patients (median 62 years, 88 men) remained on pemafibrate therapy for a median of 566 days without any drug-related adverse events. During a 3-month therapeutic period, triglyceride levels improved significantly from 302 (205, 581) mg/dL to 178 (117, 253) mg/dL (p < 0.001). During the overall therapeutic period, there were nine cardiovascular events. Comorbid chronic heart failure, comorbid coronary disease, and a lower pemafibrate dosing were independently associated with the primary endpoint (p < 0.05 for all). Those with multiple risk factors (N = 30) had a significantly higher cumulative incidence of the primary endpoint as compared with others (27% versus 3%, p < 0.001). Conclusion: pemafibrate significantly improves hypertriglyceridemia. A higher dose of pemafibrate should be considered to reduce the risk of adverse cardiovascular events, particularly in patients with chronic heart failure or coronary disease.
The Assessment of Immune Fitness
Authors: Verster Joris C. , Kraneveld Aletta D. , Garssen Johan
Published: December 2022

Immune fitness (i.e., adequate functioning of the immune system) is essential to maintain health, prevent and resolve disease, and improve quality of life. This article provides an overview of how to assess immune fitness. It discusses how a single-item rating scale can be used to assess immune fitness. The scale can be used in conjunction with a single “yes” or “no” question asking whether the individual is experiencing reduced immune fitness. Retrospective assessments can be complemented with the Immune Status Questionnaire (ISQ) to provide more insight into the type and frequency of experiencing specific immune-related complaints. Momentary assessments of immune fitness can be complemented with biomarker measurements in body fluids. As individuals may be unaware of systemic inflammation (e.g., biomarker concentrations outside the normal range), it remains critical to combine immune fitness assessments with biomarker measurements of immune functioning.
Resistance to Somatostatin Analogs in Italian Acromegaly Patients: The MISS Study
Authors: Berton Alessandro Maria , Prencipe Nunzia , Bertero Luca , Baldi Marco , Bima Chiara , Corsico Marina , Bianchi Antonio , Mantovani Giovanna , Ferraù Francesco , Sartorato Paola , Gagliardi Irene , Ghigo Ezio , Grottoli Silvia
Published: December 2022

Approximately 60% of acromegaly patients are not adequately controlled by first-generation somatostatin receptor ligands. This multicenter retrospective study aimed to identify the most relevant biomarkers specific for the Italian acromegaly population. Resistant patients were enrolled consecutively based on time of neurosurgery, while responders were collected in a 1:2 ratio. Clinical characteristics and T2-intensity on MRI scans at diagnosis were retrospectively re-evaluated. Histological analyses of CAM5.2 granulation patterns and SSTR2 expression were centrally performed. Sixty-three resistant patients and thirty-three responders were enrolled. A low-grade SSTR2 expression was the most relevant predictor of resistance identified (OR 4.58, p = 0.013), even considering CAM5.2 immunohistochemistry (OR 2.65, p = 0.047). T2-iso/hyperintense pattern on MRI was also associated with a 3.3-fold greater probability of poor response to medical treatment (p = 0.027), as well as a young age at diagnosis (OR 0.96, p = 0.035). In those patients treated only after neurosurgery due to persistent GH-hypersecretion (51, 53.1%) the absence of any appreciable adenomatous remnant on postoperative MRI was associated with a negligible risk of resistance (OR 0.04, p = 0.003). In the Italian acromegaly population, a low-grade SSTR2 expression seems to be the most relevant predictor of resistance to first-generation somatostatin receptor ligands, followed by a SG/intermediate cytokeratin pattern and a T2-iso/hyperintense MRI signal.
Oral Intake of the Commercial Probiotic Blend Synbio® for the Management of Vaginal Dysbiosis
Authors: Pino Alessandra , Rapisarda Agnese Maria Chiara , Vaccalluzzo Amanda , Sanfilippo Rosamaria Roberta , Coman Maria Magdalena , Grimaldi Raffaela Luisa , Caggia Cinzia , Randazzo Cinzia Lucia , Russo Nunziatina , Panella Marco Marzio , Cianci Antonio , Verdenelli Maria Cristina
Published: December 2022

A healthy vaginal microbiota is Lactobacillus-dominated. Several factors can interfere with the state of balance leading to dysbiosis, such as vaginal infections caused by bacteria and Candida species. The present single-arm, uncontrolled open-label study aimed to evaluate the ability of the SYNBIO® probiotic combination, taken as an oral formulation, to contribute to vaginal health. Thirty pre-menopausal participants were included in the study. Participants were instructed for daily oral intake of SYNBIO® probiotic capsules for 15 days. Vaginal swabs were collected at baseline (T0), 15 days after the start of the treatment (T1), and 7 days after the end of the treatment (T2). Amsel criteria, Nugent score, and vaginal pH were evaluated at each sampling time. In addition, the participants’ quality of life was assessed by the WHOQOL-BREF questionnaire. The administration of SYNBIO® once daily for 15 days resulted in a substantial improvement in the vaginal flora in terms of an increase in lactobacilli and a decrease in enterococci, staphylococci, Gardnerella spp., and Candida spp. According to the results, statistically significant changes in leucorrhoea, itching, and vulvo-vaginal erythema/edema as well as a decrease in all the Amsel criteria were recorded. The oral consumption of SYNBIO® demonstrated enhanced benefits for vaginal health.
Epidemiological Characteristics and Factors Associated with Alzheimer’s Disease and Mild Cognitive Impairment among the Elderly in Urban and Rural Areas of Hubei Province
Authors: Cheng Jing , Ji Xiaoqi , He Lu , Zhang Yutong , Xiao Tongtong , Geng Qiang , Wang Zhihui , Qi Shige , Zhou Fang , Zhan Jianbo
Published: December 2022

Utilize the prevalence, associated factors and population distribution of AD and MCI among residents of the Hubei province aged 60 years or over to prove that elderly people who study and communicate with others, take part in regular physical exercise and choose a healthy lifestyle, will prevent or slow the decline in cognitive ability. If elderly people study and communicate with others, take part in regular physical exercise and choose a healthy lifestyle, can prevent or slow the decline in cognitive ability. A cross-sectional study was used for the recruitment of subjects. The screened patients with AD and MCI were then selected as patients in a case–control study. A total of 4314 subjects were recruited into the study. The prevalence of AD and MCI was 1.44% and 10.04%, respectively. The prevalence of AD and MCI differed significantly as a function of age and gender (p < 0.05). The preventative factors for AD and MCI, separately, included a happy marriage (OR = 0.69, 95%CI: 0.36–1.35) and higher education (OR = 0.65, 95%CI: 0.55–0.78). The risk factors for AD and MCI, separately, included infrequent participation in social activities (OR = 1.00, 95%CI: 0.60–1.66) and infrequent communication with children (OR = 1.35, 95%CI: 1.09–1.69). The prevalence of AD for people aged 60 or over in the Hubei province was lower than the national average of 3.06%. The prevalence of MCI was within the national range (5.2–23.4%). The influencing factors of AD and MCI were associated with the participants’ social connections, lifestyle behaviors, somatic diseases and so on. The elderly people who study and communicate with others, take part in regular physical exercise and choose a healthy lifestyle will prevent or slow the decline in cognitive ability. The conclusion section has been replaced.
Surgical and Oncological Outcomes of En-Bloc Resection for Malignancies Invading the Thoracic Spine
Authors: Novellis Pierluigi , Cannavò Luca , Lembo Rosalba , Evangelista Andrea , Dieci Elisa , Giudici Veronica , Veronesi Giulia , Luzzati Alessandro , Alloisio Marco , Cariboni Umberto
Published: December 2022

Objective(s): There is still limited data in the literature concerning the survival of patients with tumors of the thoracic spine. In this study, we analyzed clinical features, perioperative and long-term outcomes in patients who underwent vertebrectomy for cancer. Furthermore, we evaluated the survival and surgical complications. Methods: We retrospectively reviewed all cases of thoracic spinal tumors treated by the same team between 1998 and 2018. We divided them into three groups according to type of tumor (primary vertebral, primary lung and metastases) and compared outcomes. For each patient, Overall Survival (OS) and Cumulative Incidence of Relapse (CIR) were estimated. Complications and survival were analyzed using a logistic model. Results: Seventy-two patients underwent thoracic spine surgery (40 in group 1, 16 in each group 2 and 3). Thirty patients died at the end of the observation at a mean follow up time of 60 months (41%). The 5-year overall survival was 72% (95% CI: 0.52–0.84), 20% (95% CI: 0.05–0.43) and 27% (95% CI: 0.05–0.56) for each group, respectively. CIR of group 3 was higher (HR 2.57, 95% CI: 1.22–5.45, p = 0.013). The logistic model revealed that age was related to complications (p = 0.04), while surgery for a type 3 tumor was related to mortality (p = 0.02). Conclusions: Although the cohort size was limited, primary vertebral tumors displayed the best 5-y-OS with an acceptable complications rate. The indication of surgery should be advised by a multidisciplinary team and only for selected cases. Finally, the use of a combined approach does not increase the risk of complications.
Length-of-Stay in the Emergency Department and In-Hospital Mortality: A Systematic Review and Meta-Analysis
Authors: Lauque Dominique , Khalemsky Anna , Boudi Zoubir , Östlundh Linda , Xu Chang , Alsabri Mohammed , Onyeji Churchill , Cellini Jacqueline , Intas Geroge , Soni Kapil Dev , Junhasavasdikul Detajin , Cabello Jose Javier Trujillano , Rathlev Niels K. , Liu Shan W. , Camargo Carlos A. , Slagman Anna , Christ Michael , Singer Adam J. , Houze-Cerfon Charles-Henri , Aburawi Elhadi H. , Tazarourte Karim , Kurland Lisa , Levy Phillip D. , Paxton James H. , Tsilimingras Dionyssios , Kumar Vijaya Arun , Schwartz David G. , Lang Eddy , Bates David W. , Savioli Gabriele , Grossman Shamai A. , Bellou Abdelouahab
Published: December 2022

The effect of emergency department (ED) length of stay (EDLOS) on in-hospital mortality (IHM) remains unclear. The aim of this systematic review and meta-analysis was to determine the association between EDLOS and IHM. We searched the PubMed, Medline, Embase, Web of Science, Cochrane Controlled Register of Trials, CINAHL, PsycInfo, and Scopus databases from their inception until 14–15 January 2022. We included studies reporting the association between EDLOS and IHM. A total of 11,337 references were identified, and 52 studies (total of 1,718,518 ED patients) were included in the systematic review and 33 in the meta-analysis. A statistically significant association between EDLOS and IHM was observed for EDLOS over 24 h in patients admitted to an intensive care unit (ICU) (OR = 1.396, 95% confidence interval [CI]: 1.147 to 1.701; p < 0.001, I2 = 0%) and for low EDLOS in non-ICU-admitted patients (OR = 0.583, 95% CI: 0.453 to 0.745; p < 0.001, I2 = 0%). No associations were detected for the other cut-offs. Our findings suggest that there is an association between IHM low EDLOS and EDLOS exceeding 24 h and IHM. Long stays in the ED should not be allowed and special attention should be given to patients admitted after a short stay in the ED.
Morphology of Anterior Cingulate Cortex and Its Relation to Schizophrenia
Authors: Lahutsina Anastasiya , Spaniel Filip , Mrzilkova Jana , Morozova Alexandra , Brabec Marek , Musil Vladimir , Zach Petr
Published: December 2022

Cortical folding of the anterior cingulate cortex (ACC), particularly the cingulate (CS) and the paracingulate (PCS) sulci, represents a neurodevelopmental marker. Deviations in in utero development in schizophrenia can be traced using CS and PCS morphometry. In the present study, we measured the length of CS, PCS, and their segments on T1 MRI scans in 93 patients with first- episode schizophrenia and 42 healthy controls. Besides the length, the frequency and the left-right asymmetry of CS/PCS were compared in patients and controls. Distribution of the CS and PCS morphotypes in patients was different from controls. Parcellated sulcal pattern CS3a in the left hemisphere was longer in patients (53.8 ± 25.7 mm vs. 32.7 ± 19.4 mm in controls, p < 0.05), while in CS3c it was reversed—longer in controls (52.5 ± 22.5 mm as opposed to 36.2 ± 12.9 mm, n.s. in patients). Non parcellated PCS in the right hemisphere were longer in patients compared to controls (19.4 ± 10.2 mm vs. 12.1 ± 12.4 mm, p < 0.001). Therefore, concurrent presence of PCS1 and CS1 in the left hemisphere and to some extent in the right hemisphere may be suggestive of a higher probability of schizophrenia.
Maternal Baseline Risk Factors for Abnormal Vaginal Colonisation among High-Risk Pregnant Women and the Association with Adverse Pregnancy Outcomes: A Retrospective Cohort Study
Authors: Jeon Junesoo , Choi Yun-sun , Kim Yejin , Hong Siryeon , Sung Ji-Hee , Choi Suk-Joo , Oh Soo-young , Roh Cheong-Rae
Published: December 2022

Abnormal vaginal colonisation can lead to adverse pregnancy outcomes such as preterm birth through intra-amniotic inflammation. Despite the concern, little is known about its risk factors and impact in pregnant women at high-risk for spontaneous preterm birth. Thus, we conducted this single-centre retrospective cohort study including 1381 consecutive women who were admitted to the high-risk pregnancy unit. The results of vaginal culture at admission were categorised according to the colonising organism: bacteria (Gram-negative or -positive) and genital mycoplasmas. Maternal baseline socioeconomic, and clinical characteristics, as well as pregnancy, delivery, and neonatal outcomes were compared according to the category. Maternal risk factors for Gram-negative colonisation included advanced maternal age, increased pre-pregnancy BMI, a greater number of past spontaneous abortions, earlier gestational age at admission, and IVF. Gram-positive colonisation was likewise associated with earlier gestational age at admission. Genital mycoplasmal colonisation was associated with a greater number of past induced abortions, a lower level of education completed, and a lower rate of multifetal pregnancy and IVF. The neonates from mothers with Gram-negative colonisation had a greater risk of NICU admission, proven early onset neonatal sepsis, and mortality. However, not Gram-positive bacteria or genital mycoplasma was directly associated with adverse pregnancy outcomes.
Numerical Analysis on Influences of Emergent Vegetation Patch on Runup Processes of Focused Wave Groups
Authors: Qu Ke , Lie Yancheng , Wang Xu , Li Xiaohan
Published: December 2022

During severe weather, the runup of the devasting surges and waves can cause extensive damages to the coastal facilities and results in significant loss of life. Therefore, it becomes necessary to find reliable measures to reduce the wave runup in coastal regions. As a natural barrier to the shorelines, coastal vegetation has great potential to attenuate wave runup and to prevent the coastal communities from extreme floodings and huge land loss. This study numerically discusses the influences of the emergent vegetation patch on the runup processes of extreme wave groups by applying a nonhydrostatic flow solver. Influences of the major factors, i.e., water depth, peak wave period, significant wave height, and density and length of the vegetation patch, are analyzed in detail. Research findings indicate that the presence of the emergent vegetation patch can substantially dissipate most of the incident wave energy and effectively reduce the maximum wave runup heights of extreme wave groups at the sloped beach. It is hoped that the research results of this study will be helpful to the design of coastal soft protection measures.
Development and Basic Qualification Steps towards an Electrochemically Based H-Sensor for Lithium System Applications
Authors: Holstein Nils , Krauss Wolfgang , Nitti Francesco Saverio
Published: December 2022

IFMIF-DONES, or the InternationalFusionMaterialsIrradiationFacility-DEMOOrientedNeutronSource, is a facility for investigations into foreseen fusion power plant materials using the relevant neutron irradiation of 14 MeV. This special n-irradiation is generated by the interaction of deuteron beams with liquid lithium. A critical issue during the operation of IFMIF-DONES is the enrichment of dissolved impurities in the Li-melt loops. The danger occurs as a result of hydrogen-induced corrosivity and embrittlement of the loop components, as well as the security hazards associated with the radioactive tritium. Hence, the application of liquid lithium in IFMIF-DONES requires a suitable impurity control system for reliable and low-level maintenance under the operating conditions of DONES. Regarding those requirements, an electrochemical sensor for hydrogen monitoring was developed in the frame of an international EUROFusion–WPENS task, to determine H-concentrations via the electro-motive force (EMF) of Li-melts and a suitable online-monitoring system. Long-term tests demonstrated that the sensor fulfills the requirements of chemical and mechanical stability and functionality under the harsh Li environment under the planned DONES conditions. Obtained results and operational experiences will be discussed in regard to application windows, reproducibility and calibration needs. Additionally, recommendations will be outlined for upgraded systems and future qualification needs.
Non-albicans Candida Species: Immune Response, Evasion Mechanisms, and New Plant-Derived Alternative Therapies
Authors: Gómez-Gaviria Manuela , Ramírez-Sotelo Uriel , Mora-Montes Héctor M.
Published: December 2022

Fungal infections caused by Candida species have become a constant threat to public health, especially for immunocompromised patients, who are considered susceptible to this type of opportunistic infections. Candida albicans is known as the most common etiological agent of candidiasis; however, other species, such as Candida tropicalis, Candida parapsilosis, Nakaseomyces glabrata (previously known as Candida glabrata), Candida auris, Candida guilliermondii, and Pichia kudriavzevii (previously named as Candida krusei), have also gained great importance in recent years. The increasing frequency of the isolation of this non-albicans Candida species is associated with different factors, such as constant exposure to antifungal drugs, the use of catheters in hospitalized patients, cancer, age, and geographic distribution. The main concerns for the control of these pathogens include their ability to evade the mechanisms of action of different drugs, thus developing resistance to antifungal drugs, and it has also been shown that some of these species also manage to evade the host’s immunity. These biological traits make candidiasis treatment a challenging task. In this review manuscript, a detailed update of the recent literature on the six most relevant non-albicans Candida species is provided, focusing on the immune response, evasion mechanisms, and new plant-derived compounds with antifungal properties.
Development of a Modified QuEChERS Method Coupled with LC-MS/MS for Determination of Spinetoram Residue in Soybean (Glycine max) and Cotton (Gossypium hirsutum)
Authors: Goon Arnab , Kundu Chiranjit , Ganguly Pritam
Published: December 2022

An analytical method for the quantitative determination of the insecticide spinetoram in cotton and soybean was established and validated using liquid chromatography tandem mass spectrometry (LC-MS/MS). Spinetoram is the mixture of two spinosyns, 3′-O-ethyl-5,6-dihydro spinosyn J and 3′-O-ethyl spinosyn L. The method involves extraction with ethyl acetate followed by dispersive solid phase extraction (dSPE) clean-up with primary secondary amine (PSA), C18 and graphitised carbon black (GCB). The final quantitation of spinetoram was done by using LC-MS/MS with positive electrospray ionization. The method was reproducible (Horwitz ratio (HorRat) < 0.5 at 25 ng g−1) and validated by the analysis of samples spiked at 25, 50 and 100 ng g−1 in soybean, cotton and soil. The recoveries of spinosyns were found to be more than 85% when spiked at different levels. The identities of spinosyns were confirmed by using the ion ratio. A field dissipation study was conducted in soybean and cotton to find out the environmental fate of spinetoram, and samples were analysed following the proposed analytical method. Both isomers were found to be dissipated quickly. The pre-harvest interval of spinetoram was calculated in different substrates.
Validations of Blood Pressure Measuring Devices Using Recognized Protocols
Authors: Mazoteras-Pardo Victoria , Gómez-Cantarino Sagrario , Ramírez-Jiménez Miguel , Navarro-Flores Emmanuel , Ugarte-Gurrutxaga María Idoia
Published: December 2022

Preventing, diagnosing, and controlling high blood pressure is a global health priority. The self-measurement of blood pressure is therefore fundamental and should be done with devices validated by recognized protocols, although most are not. The most widely used and current protocols are the 2010 European Society of Hypertension (ESH) revision and the 2018 Association for the Advancement of Medical Instrumentation (AAMI)/ ESH/ the International Organization for Standardization (ISO) universal standard, respectively. The aim of this study was to find out which blood pressure measuring devices have been adequately validated by the above protocols. A narrative review of blood pressure device validations was conducted by searching the PubMed database. From 52 records identified, 37 studies were included. Most validations follow the 2010 revision and only six follow the 2018 protocol, which is more demanding. Almost all validated sphygmomanometers are automated oscillometric sphygmomanometers in the general population. Wrist devices and devices combining new technologies are also validated, as well as in specific populations, such as the obese, pregnant women, or children. There is sufficient evidence to confirm that the universal AAMI/ ESH/ISO standard is considered the protocol of the century. However, it is necessary to increase the number of validations following it and, above all, validations of the new technologies that are invading the current market.
A Supply-Demand Framework for Eco-Compensation Calculation and Allocation in China’s National Key Ecological Function Areas—A Case Study in the Yangtze River Economic Belt
Authors: Song Mingjie , Huang Doudou , Paudel Basanta
Published: December 2022

China’s National Key Ecological Function Areas (NKEFAs) provide important ecosystem services but lose significant development opportunities. An NKEFA consists of a few National Key Ecological Function Counties (NKEFCs). China’s central government annually makes fiscal transfers to NKEFCs to compensate for their fiscal imbalance and ecosystem protection costs. The eco-compensation coefficient (ECC), together with the fiscal revenue and expenditure gap (FREG), determines the transfer payment, but the central government fails to provide practical methods for its estimation. This article proposes a framework for ECC estimation by integrating ecosystem service supply (ESS), ecosystem protection cost (EPC), and public service provision capability (PSC) of NKEFCs, and clarifies the criteria and indicators for ESS, EPC, and PSC evaluation. The framework was implemented in the Yangtze River Economic Belt (YZEB), and the results were compared with the payments in the current central-to-local fiscal transfer (CTLFT) system. The key findings and conclusions include: (1) The payment in the current CTLFT system mainly depends on the FREG rather than ESS and EPC of NKEFCs. (2) Some counties are notably under-compensated because their ESS or EPC are underestimated, or the province that administers them has a stronger fiscal capability. (3) The framework contributes to fair allocation and efficient use of eco-compensation payments by improving the ECC estimation method and identifying the main stressors and public service weaknesses in NKEFAs. This study gives the following policy implications: (1) Inner-provincial and cross-provincial watershed eco-compensation programs need to be developed to supplement the central-to-local eco-compensation program in the YZEB. (2) Environmental management strategies should be based on the characteristics of stressors and people’s livelihood in NKEFAs.
Integrating Short Rotation Woody Crops into Conventional Agricultural Practices in the Southeastern United States: A Review
Authors: Ile Omoyemeh J. , McCormick Hanna , Skrabacz Sheila , Bhattacharya Shamik , Aguilos Maricar , Carvalho Henrique D. R. , Idassi Joshua , Baker Justin , Heitman Joshua L. , King John S.
Published: December 2022

One of the United Nations Sustainable Development Goal’s (SDGs) aims is to enhance access to clean energy. In addition, other SDGs are directly related to the restoration of degraded soils to improve on-farm productivity and land management. Integrating Short Rotation Woody Crops (SRWC) for bioenergy into conventional agriculture provides opportunities for sustainable domestic energy production, rural economic development/diversification, and restoration of soil health and biodiversity. Extensive research efforts have been carried out on the development of SRWC for bioenergy, biofuels, and bioproducts. Recently, broader objectives that include multiple ecosystem services, such as carbon sequestration, and land mine reclamation are being explored. Yet, limited research is available on the benefits of establishing SRWC on degraded agricultural lands in the southeastern U.S. thereby contributing to environmental goals. This paper presents a literature review to (1) synthesize the patterns and trends in SWRC bioenergy production; (2) highlight the benefits of integrating short rotation woody crops into row crop agriculture; and (3) identify emerging technologies for efficiently managing the integrated system, while identifying research gaps. Our findings show that integrating SRWC into agricultural systems can potentially improve the climate of agricultural landscapes and enhance regional and national carbon stocks in terrestrial systems.
A UNESCO Site as a Tool to Promote Local Attractiveness: Investigating Stakeholders’ Opinions
Authors: Peira Giovanni , Pasino Giacomo , Bonadonna Alessandro , Beltramo Riccardo
Published: December 2022

UNESCO World Heritage Sites are a useful tool to protect and promote the legacy human beings leave over the centuries. Ivrea, a 20th century industrial city, is the second Italian industrial site recorded in the UNESCO World Heritage List. It is a city in the North-West of Italy, historically known for the Olivetti factory, which made it world-famous by combining visionary ideals and a careful entrepreneurial policy. Our study focuses on the tourism value of the Ivrea UNESCO site and, in particular, aims at analyzing the level of integration of local stakeholders and their ability to identify guidelines for the promotion of the UNESCO site in terms of tourism. The research process was conducted with an in-depth analysis of the perception and consideration of public and private stakeholders concerning this topic. A questionnaire was prepared by a group of experts and individual interviews were conducted using the Delphi method; then, the results of the interviews were presented and the identification of priorities was carried out using the nominal group technique. Results show that the new UNESCO site in Ivrea has great potential, both in cultural terms, continuing the dissemination of Adriano Olivetti’s ideals, and in terms of tourism, because it may turn into a tourist attraction capable of generating new tourist flows and promoting the Olivetti approach.