bluuebunny/crossref_metadata_2025 huggingface dataset:
RELATIONSHIP BETWEEN WORK POSTURE AND REPETITIVE MOVEMENTS WITH COMPLAINTS OF CARPAL TUNNEL SYNDROME (CTS) ON STONE-BREAKING WORKERS ON THE BANKS OF THE KALISETAIL RIVER. SETAIL VILLAGE, GENTENG DISTRICT, BANYUWANGI REGENCY
Authors: Kumalasari Ulviana Dewi , Dwiyanti Endang
Published: September 2022

ABSTRACT
 Background: Workers who perform repetitive movements and work in a manner that remains seated for a long time experience Carpal Tunnel Syndrome in stone-breaking workers. It can reduce worker productivity because CTS can cause disability. Objective: Analyzing the relationship between work posture and repetitive movements with Carpal Tunnel Syndrome in stone-breaking workers on the banks of the Kalisetail River, Genteng District, Banyuwangi Regency. Method: This study was included in an observational analytic study with a cross-sectional research design. The samples in this study were all stone-breaking workers, consisting of 30 people, along the Kalisetail River, Setail Village, Genteng District, and Banyuwangi Regency. Results: The results showed the relationship between work posture (r = 0.420) and repetitive movement (r = 0.671) with complaints of Carpal Tunnel Syndrome in stone-breaking workers on the banks of the Kalisetail River was sufficient or moderate until strong. Conclusion: This study concludes that the relationship between work posture and repetitive movements of Carpal Tunnel Syndrome was sufficient until strong in stone-breaking workers on the outskirts of Kalisetail River. The researcher suggests applying rest breaks or recovery times, physical exercise during break times, and workplace adjustment.
ABSTRAK
Latar Belakang: Pekerja yang melakukan gerakan repetitif dan bekerja dengan posisi tetap yaitu duduk dalam waktu yang lama merupakan penyebab terjadinya Carpal Tunnel Syndrome pada pekerja pemecah batu. Hal ini dapat menurunkan produktivitas pekerja karena CTS dapat menimbulkan kecacatan. Tujuan: Menganalisis hubungan antara postur kerja dan gerakan repetitif dengan Carpal Tunnel Syndrome pada pekerja pemecah batu di pinggiran Sungai Kalisetail Kecamatan Genteng Kabupaten Banyuwangi. Metode: Penelitian ini termasuk dalam penelitian analitik observasional dengan desain penelitian cross sectional. Sampel dalam penelitian ini adalah semua populasi pekerja pemecah batu di sepanjang Sungai Kalisetail Desa Setail, Kecamatan Genteng, Kabupaten Banyuwangi sebanyak 30 orang. Hasil: Hasil penelitian menunjukkan bahwa hubungan antara postur kerja (r=0,420 dan gerakan repetitif (r=0,671) dengan keluhan Carpal Tunnel Syndrome pada pekerja pemecah batu di pinggiran Sungai Kalisetail adalah cukup atau sedang sampai kuat. Kesimpulan dari penelitian ini adalah hubungan antara postur kerja dan gerakan repetitif dengan keluhan Carpal Tunnel Syndrome adalah cukup atau sedang sampai kuat pada pekerja pemecah batu di pinggiran Sungai Kalisetail Kecamatan Genteng Kabupaten Banyuwangi. Saran peneliti adalah penerapan jeda istirahat atau waktu pemulihan, latihan fisik di sela waktu istirahat, dan penyesuaian tempat kerja.
ANALYSIS OF USE, AVAILABILITY OF PERSONAL PROTECTION EQUIPMENT (PPE) AND COVID-19 INFECTIONS CASE ON HEALTH WORKERS : A LITERATURE REVIEW
Authors: Putri Alifia Fiarnanda , Adriyani Retno , Haibah Anizah Izzi , Hamedon Titi Rahmawati
Published: September 2022

ABSTRACT
COVID-19 is an infectious disease caused by the SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) virus. During the pandemic, health workers have a higher risk of being exposed to the coronavirus. This study aims to analyze the availability and use of PPE for COVID-19 infection cases in health workers. This study used the scoping review method. Selected articles had been chosen by topic and inclusion criteria. Twenty-four articles were varied based on research locations in the US, China, Italy, Germany, Ethiopia, India, Pakistan, Nigeria, Australia, and Israel. Health workers have used PPE when handling specimens or patients with COVID-19 symptoms. The health workers were varied, including doctors, dentists, veterinarians, public health officers, nurses, pharmacists, and medical personnel who treat COVID-19 patients or not—the type of PPE widely used as masks. Health care facilities have provided PPE, but access, quality, and availability vary. Cases of COVID-19 infection in health workers varied, and the symptoms. PPE availability indirectly affects the high or low cases of COVID-19 infection in health workers, so the availability of PPE for health workers must be considered.
ABSTRAK
COVID-19 merupakan penyakit menular yang disebabkan oleh virus SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2). Selama pandemi, tenaga kesehatan memiliki risiko lebih tinggi terpapar virus corona. Tujuan dari penulisan ini yaitu menganalis ketersediaan dan penggunaan APD terhadap kasus infeksi COVID-19 pada tenaga kesehatan. Penelitian ini menggunakan metode scoping review. Artikel yang dipilih sesuai dengan topik dan kriteria inklusi. Didapatkan 24 artikel dengan lokasi penelitian di AS, Cina, Italia, Jerman, Ethiopia, India, Pakistan, Nigeria, Australia, dan Israel. Tenaga kesehatan telah menggunakan APD saat menangani pasien ataupun spesimen pasien dengan gejala COVID-19. Tenaga kesehatan yang diteliti bervariasi, meliputi dokter, dokter gigi, dokter hewan, public health officer, perawat, apoteker, tenaga medis yang menangani pasien COVID-19 ataupun tidak. Jenis APD yang paling banyak digunakan oleh tenaga kesehatan yaitu masker. Fasilitas pelayanan kesehatan telah menyediakan APD, namun akses, kualitas, dan ketersediaannya bervariasi. Kasus infeksi COVID-19 pada tenaga kesehatan bervariasi, begitu pula dengan gejala yang timbul. Penggunaan APD dapat meminimalisir risiko penularan COVID-19 pada tenaga kesehatan. Ketersediaan APD berpengaruh tidak langsung terhadap tinggi atau rendahnya kasus infeksi COVID-19 pada tenaga kesehatan, sehingga ketersediaan APD untuk tenaga kesehatan harus diperhatikan.
Networked scheduling for decentralized load frequency control
Authors: Peng Chen , Yang Hongchenyu
Published: September 2022

This paper investigates the scheduling process for multi-area interconnected power systems under shared but band-limited networks and decentralized load frequency controllers. To cope with sub-area information and avoid node collision of large-scale power systems, round-robin and try-once-discard scheduling are used to schedule sampling data among different sub-grids. Different from existing decentralized load frequency control methods, this paper studies multi-packet transmission scheme and introduces scheduling protocols to deal with multi-node collision. Considering the scheduling process and decentralized load frequency controllers, an impulsive power system closed-loop model is well established. Furthermore, sufficient stabilization criteria are derived to obtain decentralized output feedback controller gains and scheduling protocol parameters. Under the designed decentralized output feedback controllers, the prescribed system performances have been achieved. Finally, a three-area power system example is used to verify the effectiveness of the proposed scheduling method.
Heat and Photon Energy Phenomena: Dealing with Matter at Atomic and Electronic Level
Authors: Ali Mubarak
Published: September 2022

There is a misconception about using the terms photon and electron. When the electron of the outer ring in the silicon atom executes interstate dynamics for only one cycle, it generates force and energy for the unit photon. When the photon of suitable length interacts with the side of the laterally orientated electron of an atom, it converts into heat energy. Under the approximate angle of 90º, when a photon interacts with the tip of a laterally orientated electron, it divides into bits of energy having a shape like integral symbols. In the neutral state silicon atom, the center acts as the reference point for electrons executing interstate dynamics, and the lateral lengths of the electrons remain along the north-south poles. The energy wraps around the force, which shapes along the tracing trajectory of electron dynamics in a silicon atom. Force shapes from only those sides of the electron, which are not in the exertion of forces. In interstate dynamics, the electron of the outer ring first reaches the maximum limit point, where the one-bit energy shapes. Electron completes the second half cycle, where the one-bit energy again shapes. The unit photon has a shape like Gaussian distribution in turned ends. When there is an uninterrupted supply of heat energy to the silicon atom, electron dynamics generate the photon having a shape-like wave. Path-independent but interstate-dependent forces take over the control of an electron. That electron executes dynamics nearly at the speed of light. In confined interstate dynamics, naturally viable conservative forces exert on the position-acquiring electron. A photon can be in unending length if the electron dynamics remain uninterrupted. The changing aspect of the electron recalls the auxiliary moment of inertia at each point of turning. By executing electron dynamics, atoms under neutral states generate photons of different shapes, revealing heat and photon energy phenomena.
Enhancing the Potential of Smart Building for General Hospital: A Case Study in Malaysian Hospital
Authors: Baharuddin Mohd Faisal , Lim Chin Haw , Fazlizan Ahmad
Published: September 2022

Hospital Pulau Pinang is the general hospital in Malaysia which targeting energy savings of 10% within five years from 2015 and other sustainability targets such as 3-star Energy Management Gold Standard and Green Building Certification. The targets are beneficial for the hospital itself to establish the Smart Building Program to improve its energy efficiency concurrent with the green policy of the Ministry of Health Malaysia and Sustainable Development Goals by the United Nations. This paper reviews the background of Hospital Pulau Pinang energy data , energy consumption trending, energy-saving trending, and energy conservation measures taken for the hospital from 2015 to December 2021.The yearly energy consumption baseline taken in 2016 was 27,496,731.00 kWh. It reduced significantly to 21,356,063 kWh in 2021 due to energy conservation measures. As a result, Hospital Pulau Pinang has achieved energy-saving about 16% at approximately RM7.3 million reduction in operational expenditure. The main objective of this paper is to provide further potential energy savings by studying the energy reduction by implementing solar photovoltaics using the simulation method. The simulation method can predict that Hospital Pulau Pinang can achieve another 5,130,000 kWh energy savings annually. This type of simulation has never been done before at a public hospital, and it will give further enhancing strategies to the Smart Building Program itself. Furthermore, the potential of smart building can be maximized to the next level by simulation, which helps the hospital energy committee make the potential decision on the energy-saving investment.
Modeling of PV Cell, PV Module, PV Array and PV IV Characteristics Analysis Using MATLAB/SIMULINK
Authors: Das Arnob , Peu Susmita
Published: September 2022

In this paper, a study of modeling PV cell (solar cell), PV module (solar modules) and PV array solar array) using Matlab/Simulink software is presented. This study is focused on the study to design photovoltaic cell, photovoltaic modules, photovoltaic panels and PV array (solar array) and to analyze the change in PV performance according to changes in different parameters such temperature, solar irradiation, reverse saturation current, series resistance and shunt resistance. This study shows the PV and IV characteristics curve for each model from which MPP (maximum power point) is determined. In this study, different PV and IV characteristic curves are gained for varying temperatures with specified solar irradiation conditions,s and then the MPP is tracked. The highest power output is also compared between the designed PV module and the selected PV module (MX Solar USA MX60-245)
Artificial Intelligence of Things Applied to Assistive Technology: A Systematic Literature Review
Authors: Freitas Maurício Pasetto de , Piai Vinícius Aquino , Farias Ricardo Heffel , Fernandes Anita M. R. , Rossetto Anubis Graciela de Moraes , Leithardt Valderi Reis Quietinho
Published: September 2022

According to the World Health Organization, about 15% of the world’s population has some form of disability. Assistive Technology, in this context, contributes directly to the overcoming of difficulties encountered by people with disabilities in their daily lives, allowing them to receive education and become part of the labor market and society in a worthy manner. Assistive Technology has made great advances in its integration with Artificial Intelligence of Things (AIoT) devices. AIoT processes and analyzes the large amount of data generated by IoT devices and applies Artificial Intelligence models, specifically Machine Learning, to discover patterns for generating insights and assisting in decision making. Based on a systematic literature review, this article aims at identifying the Machine Learning models used in multiple different research about Artificial Intelligence of Things applied to Assistive Technology. The survey of the topics approached in this article also highlights the context of such research, their application, IoT devices used, and gaps and opportunities for further development. Survey results show that 50% of the analyzed research address visual impairment, and for this reason, most of the topics cover issues related to computational vision. Portable devices, wearables, and smartphones constituted the majority of IoT devices. Deep Neural Networks represent 81% of the Machine Learning models applied in the reviewed research.
Training on Emotional Intelligence for Caregivers of Patients with Acquired Brain Injury and Cognitive Impairment: A Quasi-Experimental Study
Authors: De-Torres Irene , Bustos Fernando , Arango-Lasprilla Juan Carlos , Fernández-Berrocal Pablo
Published: September 2022

Background: Cognitive-behavioral alterations can occur after an acquired brain injury. It is a stressful situa-tion for patient and relatives. Objectives: To develop and evaluate a synchronous online training program on emotional intelligence (EI) for caregivers of adult patients with cognitive-behavioral impairment due to acquired brain injury. Methods: A quasi-experimental study was designed, a target population of ten caregivers attended to a one-month virtual synchronous course about EI. The emotional status of caregivers was registered one-month-previous and one-month-post program using comparative measures: The Trait Meta-Mood Scale (TMMS-24) and the Positive and Negative Affect Schedule (PANAS). Results: Median age of the ten caregivers was 48 years, 80% of women with a median care-time of 6 years. 50% of them were spouses of the patients. 60% of the patients were affected by stroke (hemorrhagic or ischemic cause). The main cognitive impairment of the patients reported by relatives was memory deficit. After re-ceiving training, favorable changes were found regarding emotional affect measured with the PANAS, both positive (increase) and negative (decrease), as well as with the TMMS-24-mood-repair area (p < 0.05). Conclusions: Training in EI helps caregivers to make their mood more positive and improve aspects of their emotional intelligence such as emotional regulation.
Bulb Yield Stability Study of Onion Lines over Locations and Seasons in Ghana and Mali
Authors: Tignegre Jean Baptiste De La Salle , Traore Alpha Sidy , Konate Moumouni , Zaato Paul Alhassan , Diarra Ba Germain , Hanson Peter , Kiziito Fred , Birhanu Birhanu Zemadim , Afari-Sefa Victor
Published: September 2022

Onion is one of the most economically and nutritionally important vegetable crops in West Africa. However, high-yielding varieties that are accessible to farmers remain scarce. Onion lines from the World Vegetable Center’s Allium program were evaluated during the cool and dry season from September to March for three years to identify adapted onion lines suitable for sustainable intensi-fication of production systems in Northern Ghana and Southern Mali. Trials were implemented in the Upper East and Northern Regions of Ghana and in two districts in the Sudan savanna zones of Mali (Bougouni and Koutiala). Nine onion lines, including a check variety were assessed for yield stability using a randomized complete block design. The trials were carried out in “technology parks” under the joint management of farmers and researchers. Onion bulb weight was recorded for each plot after harvest. Separate analyses of variances were performed for each location and season. Analysis of variance of combined locations, seasons and lines was made to determine the most stable varieties using the line-superiority measure and ecovalence stability coefficients. Results indicated that lines AVON1310 and AVON1325 were most stable for yield performance over lo-cations and seasons. From the genotype main effects and genotype-by-environment interaction (GGE) biplot, the best performing lines were AVON1310 (33.32 t.h-1), AVON1308 (28.81 t.h-1) and AVON1325 (31.68 t.h-1). These lines are potential candidates for sustainable intensification of onion production in Ghana and Mali.
DERECHO PROCESAL CONSTITUCIONAL: UNA PERSPECTIVA COMPARADA
Authors: Ovalle Favela José
Published: September 2022

Es un análisis histórico y comparativo del surgimiento y desarrollo de los tres principales sistemas de control jurisdiccional de la constitucionalidad de las leyes: el norteamericano, el austríaco y el francés. De cada uno de estos se estudian sus antecedentes, sus fundamentos doctrinales y jurídicos, su evolución y sus principales características.
Diversity and Inclusion in Orthopaedic Surgery from Medical School to Practice
Authors: Lamanna Daniel L. , Chen Antonia F. , Dyer George S.M. , Johnson Anthony E. , McCarthy Colm J.
Published: September 2022

Orthopaedic surgery has historically been one of the least diverse fields in medicine in the U.S. Despite having been declared a critical issue by the American Orthopaedic Association, a lack of diversity and inclusion across all metrics remains a persistent problem. In order to balance representation within orthopaedic surgery, a multiyear and multilevel approach should be considered across the life cycle of a surgeon. Talented individuals from diverse backgrounds must be identified and given early exposure to orthopaedic surgery. They must be nurtured, mentored, and retained. Representation at the medical student, resident, and faculty level is vital to ensuring diversity across the next generation of surgeons.

We cannot alter representation within orthopaedics unless we broaden the candidate selection pool. Medical school classes provide the selection pool for residency, residency provides the candidates for fellowship, fellowships provide the candidates for employment, and employment provides the pool for promotion to leadership positions. Through each progression, there is a loss of underrepresented applicants, which leads to a lack of balanced representation in orthopaedic surgery. With stronger efforts to identify and retain individuals at each phase of a surgeon’s career, we hope to mitigate the loss of talented and diverse individuals from this field.

We challenge the paradigm of increasing diversity that focuses only on the resident selection level. Instead, efforts must begin at the medical student level. Efforts for early and meaningful exposure to the field through a musculoskeletal curriculum and rotations as well as connection through mentorship and sponsorship are vital for retention. At each ascending level of education, reinvestment in each individual is critical. Exposure, mentorship, retention, and promotion should lead to a more diverse and rich future. To achieve this, deliberate and longitudinal action should be instituted to increase diversity within orthopaedics.
Evaluation of climatic and hydroclimatic resources to support the US Army Corps of Engineers Regulatory Program
Authors: Sparrow Kent , Gutenson Joseph , Wahl Mark , Cotterman Kayla
Published: September 2022

Short-term climatic and hydrologic interactions, or hydroclimatology, are an important consideration when delineating the geographic extent of aquatic resources and assessing whether an aquatic resource is a jurisdictional water of the United States (WOTUS) and is therefore subject to the Clean Water Act (CWA). The now vacated 2020 Navigable Waters Protection Rule (NWPR) required the evaluation of precipitation and other hydroclimatic conditions to assess the jurisdictional status of an aquatic resource based on normal hydroclimatic conditions. Short-term hydroclimatic conditions, such as antecedent precipitation, evapotranspiration, wetland delineation, and streamflow duration assessments, provide information on an aquatic resource’s geo-graphic extent, hydrologic characteristics, and hydrologic connectivity with other aquatic resources. Here, researchers from the US Army Corps of Engineers, Engineer Research and Development Center (ERDC) evaluate tools and data available to practitioners for assessing short-term hydroclimatic conditions. The work highlights specific meteorological phenomena that are important to consider when assessing short-term hydroclimatic conditions that affect the geographic extent and hydrologic characteristics of an aquatic resource. The findings suggest that practitioners need access to data and tools that more holistically consider the impact of short-term antecedent hydroclimatology on the entire hydrologic cycle, rather than tools based solely on precipitation.
Dietary Crude Protein and Total Digestible Nutrient on The Performance of Boerka Goats Male Growing Phase
Authors: Hutasoit Rijanto , Solehudin Solehudin , Ginting SP , Simanihuruk K , Zubaidah S , Sumarni Sumarni
Published: September 2022

Boerka goat is the best meat type goat. Feed quality is one of the biggest factors that affect productivity, so it is necessary to know in advance the need for nutrients. The purpose of this study was to determine the dietray of crude protein (CP) and Total Digestible Nutrients (TDN) in efficient feed for the production of Boerka goats male growing phase. This study was designed in a completely randomized design, consisting of six combination feed treatments with six replications. Namely: 1 (CP 13.00% and TDN 63.10%); 2 (CP 13.03% and TDN 65.07%); 3 (CP 13.00% and TDN 70.00%); 4 (CP 15.00% and TDN 62.69%); 5 (CP 15.12% and TDN 65.00%); 6 (CP 15.00% and TDN 70.00%). The observed variables were feed consumption, feed consumption ratio (FCR), nutrient digestibility (dry matter, organic matter, crude protein) and average daily gain (ADG). Results of this study show that the balanced of CP and TDN has no significant effect on consumption and FCR. However, it was significantly different for digestibility and ADG. Feed treatment (CP 13.03% and TDN 65.07%) obtained the higher digestibility of DM and OM (71.49 and 70.5%), while the highest CP digestibility was treated with CP 15.05% and TDN 62.69% (71.59%). The ADG response of Boerka goats with CP 13.03% and TDN 65.07% was the good results (144.90 g head-1day-1) while the lowest response was on CP 13.08% and TDN 63.10%. It is concluded that the dietray of CP 13.03% and TDN 65.07% is a recommended of Boerka goat male growing phase.
The Level of Community Compliance Towards Prevention Efforts of COVID-19 in West Tanjung Jabung Regency, Jambi
Authors: Zulfitri Zulfitri , Rahman Safrizal , Badri Misbahul , Zulkifli Baidillah
Published: September 2022

The COVID-19 cases in Tanjung Jabung Barat Regency, Jambi, have has risen significantly daily. This significant increase in cases illustrates that the people are less than optimal in complying with the health protocols established by the government. This phenomenon concluded that public compliance in efforts to prevent COVID-19 has a vital role in preventing the spread. This study aims to describe the level of compliance towards prevention efforts of COVID-19 in the community. We use a descriptive survey method with probability sampling techniques to obtain the data. The subjects in this study were the community of Tanjung Jabung Barat, Jambi Regency, who were17 years old with a sample size of 400 samples. Collecting data using a questionnaire in a validated form and distributed to the public via social media. The data analysis in this study is the frequency distribution analysis (univariate analysis). The results of this study shown were that 172 respondents (43.0%) had a good level of compliance, 146 respondents (36.5%) had a sufficient level of adherence, and as many as 82 respondents (20.5%) had a good level of compliance to prevent COVID-19 spreads.
Detection Of Antibiotic Residues in Eggs of Layer Chickens and Knowledge of Animal Officers About Antibiotics
Authors: Jefrianda Jefrianda , Nurliana Nurliana , Darmawi Darmawi , Ferasyi Teuku Reza , Sugito Sugito
Published: September 2022

The chicken farming industry in Indonesia cannot be separated from the use of antibiotics as a curative effort in controlling the disease. Its use is either via injection, soaking, or as a feed additive (growth promoter) mixed in feed. Inappropriate use of antibiotics causes allergic reactions or resistance and the possibility of causing poisoning, residues in livestock tissues or organs that are harmful to human health when consuming them. This study aimed to determine the antibiotic residues in eggs and the level of knowledge of livestock workers on the use of antibiotics in livestock. The detection of antibiotic residues was conducted using the agar plate diffusion method by Kirby Bauer with the disk diffusion technique using Escherichia coli and Salmonella sp. As indicator bacteria. The results were analysed using a qualitative descriptive approach. The samples were 76 eggs taken from the Regional Technical Implementation Unit of the Non-Ruminant Livestock Center of the Aceh Livestock Service in Blang Bintang and Saree Aceh Besar. Chloramphenicol antibiotic was used as a test medium and as control by calculating the diameter of the inhibition zone formed on MHA media. furthermore, a cross-sectional study design was used and questionnaires were distributed to 14 livestock officers to understand the knowledge of officers on the use of antibiotics in livestock by using google form. The data were analyzed using the chi-square test through the SPSS 22.0 program. The results showed that the eggs and feed samples were negative from antibiotic residue. There was no relationship between feeding and antibiotic residues in eggs and laying hens feed. However, only 35.7% of officers had good knowledge about proper antibiotic usage. This means continuing awareness to the officers regarding the use of antibiotics either for treatment of diseases or as feed promoter are essential.
The effects of abscisic acid administration in PCOS rat model and H202 induced human granulosa cell damage: A preliminary investigation
Authors: Xie Yidong , Li Xiaohong , Guo Junliang , Xiao Li , Li Shangwei
Published: September 2022

Background
Polycystic ovary syndrome (PCOS) is commonly associated with metabolic disorders. Abscisic acid (ABA) is a phytohormone present in vegetables and fruits that can be naturally supplied by the dietary intake and has been previously studied for its benefits to human health, especially in diabetes people. It has been demonstrated that ABA plays a key role in glucose metabolism, inflammation, and tumor growth. The aim of this study was to investigate the therapeutic effect of ABA on letrozole-induced PCOS rats.
Materials and Methods
Wistar rats were implanted with letrozole-continuous-release pellets to induce a PCOS-like phenotype, subsequently treated with ABA or vehicle control. Bodyweight changes, Testosterone(T) level, fasting insulin measurements, and glucose tolerance tests have been investigated. A cell apoptosis model was established induced by H2O2. Cell viability was tested by cell counting Kit-8 (CCK-8). Cell apoptosis was tested by flow cytometry, and the oxidative stress state of cells was examined using ROS array.
Results
Body weights, glucose impairment, and T level were significantly improved in the ABA treated PCOS rat than in vehicle-treated control PCOS rats. ABA significantly reduced H2O2-induced KGNs cell apoptosis and ROS levels and increased cell viability.
Conclusion
The findings suggest that ABA could be a candidate adjunctive treatment for improving the insulin sensitivity and hyperandrogenemia of PCOS patients, and ameliorated H2O2-induced KGN cell apoptosis and oxidant stress.
Phenotypic and functional characterisation of locally produced natural killer cells ex vivo expanded with the K562-41BBL-mbIL21 cell line
Authors: Shman Tatsiana , Vashkevich Katsiaryna , Migas Aleksandr , Matveyenka Mikhail , Lasyukov Eugeniy , Muhametshina Anastasia , Horbach Katsiaryna , Aleinikova Olga
Published: September 2022

We characterise the expansion, phenotype and functional activity of natural killer (NK) cells obtained for a clinical trial.
Nineteen expansion procedures were performed to obtain NK cell products for 16 patients. NK cells were ex vivo expanded from haploidentical donor peripheral blood mononuclear cells in the presence of the locally generated feeder cell line K-562 with ectopic expression of 4-1BBL and mbIL-21.
The median duration of expansion was 18 days (range 14–25). The median number of live cells yielded was 2.26 × 109 (range 0.89–5.5 × 109) with an NK content of 96.6% (range 89.0%–98.8%). The median NK cell fold expansion was 224.7 (range 42–647). The majority of expanded NK cells had the phenotype of immature activated cells (NKG2A+, double bright CD56++CD16++, CD57-) expressing NKp30, NKp44, NKp46, NKG2D, CD69, HLA-DR and CD96. Despite the expression of exhaustion markers, expanded NK cells exhibited high cytolytic activity against leukaemia cell lines, high degranulation activity and production of cytokines. There was noted decreased functional activity of NK cells in tests against the patient’s blasts.
NK cells obtained by ex vivo expansion with locally generated K562-41BBL-mbIL21 cells have both a relatively undifferentiated phenotype and enhanced cytolytic activity against cancer cell lines. Expansion of NK cells with the feeder cells allows obtaining a sufficient quantity of the NK cell product to reach high cell doses or increase the frequency of cell infusions for adoptive immunotherapy. Registered at clinicaltrials.gov as NCT04327037.
Long-term survive of Aliarcobacter butzleri in two models symbiotic interaction with Acanthamoeba castellanii
Authors: Medina Gustavo A. , Flores-Martin Sandra N. , Pereira Wellison A. , Figueroa Elías G. , Guzmán Neftalí H. , Letelier Pablo J. , Andaur Marcela R. , Leyán Pilar I. , Boguen Rodrigo E. , Hernández Alfonso H. , Fernández Heriberto
Published: September 2022

Aliarcobacter butzleri (formerly known as Arcobacter butzleri) is an emerging foodborne zoonotic pathogen that establishes in vitro endosymbiotic relationships with Acanthamoeba castellanii, a free-living amoeba. Previously, we described that this bacterium acts as an endocytobiont of A. castellanii, surviving for at least 10 days in absence of bacterial replication. Thus, the aim of this study was to evaluate the ability of A. butzleri to survive as a long-term endosymbiont of A. castellanii for 30 days in two models of symbiotic interaction with A. castellanii: i) endosymbiotic culture followed by gentamicin protection assay and ii) transwell co-culture assay. The results allow us to conclude that A. butzleri is capable of surviving as an endosymbiont of A. castellanii for at least 30 days, without multiplying, under controlled laboratory conditions. In addition, in the absence of nutrients and as both microorganisms remain in the same culture, separated by semi-permeable membranes, A. castellanii does not promote the survival of A. butzleri, nor does it multiply. Our findings suggest that the greater survival capacity of A. butzleri is associated with their endosymbiont status inside A. castellanii, pointing out the complexity of this type of symbiotic relationship.
Social drivers of short stay market in a regional Australia: A local development perspective
Authors: Ndaguba Emeka , Brown Kerry , Marinova Dora , Jogulu Uma
Published: September 2022

Discourse on microentrepreneurs research on short stay accommodation was not in existence before 2008. After 2009, several concepts and constructs were developed and a few till date have been empirically tested, but a majority of those that have been tested relate to economics, leaving the social, cultural, and environmental perspective backward. Thus, this study aims to identify and assess social factors influencing short-stay market economy in a regional area. 16 interview sessions with 18 participants of a face-to-face interview conducted, and the Leximancer software was employed for its analysis. The study shows that the social drivers to SSA are not generic, but pluralistic, multidimensional, and dynamic, and that limiting regulatory clauses to borrowed concepts of limitation or prohibition, the council demonstrates a lack of digital leadership. Despite ample opportunities provided by SSM in regional area, there are few encumbrances that cannot be overlooked.
WITHDRAWN: Benchmarking Fitness Center Business Performance in Thailand: Efficiency Assessment with Data Envelopment Analysis
Authors: Chankuna Dittachai , Thanaiudompat Thanarit , Sukdee Thitipong
Published: September 2022

Fitness center business (FCB) in Thailand has increasing marketing value and economic impacts to sports industry for the last years. Despite the fact that, sport marketeers often enhance competitiveness by increases investment through various marketing strategies, efficiency never assessed. Data envelopment analysis (DEA) and then Tobit regression model were firstly applied in FCB for benchmarking their efficiency and helps entrepreneurs, marketeers, and scholars fulfil the management gap to investigated the influenced marketing factors affected to the FCB performance. 60 FCBs around Thailand was qualified the inclusion criteria to be a decision-making unit (DMU). Average efficiency score of DMUs is 0.9468, which consistent with empirical data that they survived after COVID-19 pandemic. Personal trainer standard, product development, and atmosphere and interior decoration are significantly influenced marketing factors affected to the FCB performance. We suggested that entrepreneur or marketeer should adopt personal trainer into marketing strategy for driving FCB performance.
The impact of micro-credit use on small-scale farmer’s income and expenditure Evidence from Raya kobo Woreda, Ethiopia
Authors: Tefera Abate Beleye , Awoke Agerwork Jejaw
Published: September 2022

Background
This study was fully conducted through primary data gained from 385 farm household heads. Those farm household heads were determined by Cochran sample size determination formula and three stage probability sampling was applied to select them. The collected data was analyzed through propensity score matching method of impact evaluation.
Result
The results of this study reveals that household income and expenditure was improved (10,934 ETB &1,705 ETB respectively) as the result of microcredit participation for the treated households.
Conclusion
Therefore any microfinance development intervention should include strategies on how to enrich and incorporate the microcredit participation of large number of small-scale farm households.
Scalable training of graph convolutional neural networks for fast and accurate predictions of HOMO-LUMO gap in molecules
Authors: Choi Jong Youl , Zhang Pei , Mehta Kshitij , Blanchard Andrew , Pasini Massimiliano Lupo
Published: September 2022

Graph Convolutional Neural Network (GCNN) is a popular class of deep learning (DL) models in material science to predict material properties from the graph representation of molecular structures. Training an accurate and comprehensive GCNN surrogate for molecular design requires large-scale graph datasets and is usually a time-consuming process. Recent advances in GPUs and distributed computing open a path to reduce the computational cost for GCNN training effectively. However, efficient utilization of high performance computing (HPC) resources for training requires simultaneously optimizing large-scale data management and scalable stochastic batched optimization techniques. In this work, we focus on building GCNN models on HPC systems to predict material properties of millions of molecules. We use HydraGNN, our in-house library for large-scale GCNN training, leveraging distributed data parallelism in PyTorch. We use ADIOS, a high-performance data management framework for efficient storage and reading of large molecular graph data. We perform parallel training on two open-source large-scale graph datasets to build a GCNN predictor for an important quantum property known as the HOMO-LUMO gap. We measure the scalability, accuracy, and convergence of our approach on two DOE supercomputers: the Summit supercomputer at the Oak Ridge Leadership Computing Facility (OLCF) and the Perlmutter system at the National Energy Research Scientific Computing Center (NERSC). We present our experimental results with HydraGNN showing i) reduction of data loading time up to 4.2 times compared with a conventional method and ii) linear scaling performance for training up to 1,024 GPUs on both Summit and Perlmutter.
Abnormal placentation: the necessity for preoperative magnetic resonance imaging
Authors: Huang Marilyn , Knickerbocker Angelica M. , Pon Fay F. , Castillo Rosa P , Galli Johnny H. , Maldonado-Puebla Marigloria , Paidas Michael , Ghulmiyyah Labib M , Sinno Abdulrahman K.
Published: September 2022

Background:Placenta accreta spectrum (PAS) is characterized by abnormal placental adherence that increases the risk of maternal hemorrhage and death. Current literature suggests lower rates of maternal morbidity and mortality when placenta accreta spectrum is diagnosed or suspected prenatally. We examined the use of preoperative imaging in predicting PAS with the goal of assessing concordance between imaging modalities and impact of differential imaging on morbidity.
Methods: Women with suspected placenta accreta spectrum who delivered at our institution from 2010-2018 were included. Perioperative diagnoses, imaging, and pathology reports were abstracted from medical records. Descriptive statistics, ANOVA, chi square, multinomial logistic regression, Cohen’s kappa analyses were performed.
Results: There were 152 cases with suspected placenta accreta spectrum from 2010-2018 with 131 (86.2%) cases confirmed by final pathology. Of the confirmed cases, there were 38 (25%) accretas, 62 (40.8%) incretas, and 31 (20.4%) percretas. Magnetic resonance imaging (MRI) more accurately diagnosed placenta accreta spectrum (39% of cases) than ultrasound (25%). Cohen’s kappa for ultrasound versus pathology was 0.02836, demonstrating slight inter-rater agreement (p=0.55) than 0.5087 in the MRI versus pathology group (p=0.08). Of the 107 patients requiring resuscitative blood products, indicators of morbidity, the percreta group had significantly greater mean estimated blood loss (4.8L) with longer mean length of hospital stay (p=0.014) compared to increta (3.1L) and accreta (2.6L) (p=0.04).
Conclusions: This study supports the role of pelvic magnetic resonance imaging for antepartum diagnosis of placenta accreta spectrum. Accurate diagnosis is essential for the perioperative coordination of care to improve maternal outcomes and reduce mortality.
The recalibration and redevelopment of a model to calculate patients’ probability of completing a colonoscopy following an abnormal fecal test
Authors: Petrik Amanda , Johnson Eric S. , Slaughter Matthew , Leo Michael C. , Thompson Jamie , Mummadi Raj , Jimenez Ricardo , Hussain Syed , Coronado Gloria
Published: September 2022

Background: Fecal immunochemical testing (FIT) is an effective screening tool for colorectal cancer. If a FIT is abnormal, a follow-up colonoscopy is necessary to remove polyps or find cancers. Identifying patients who have a low probability of obtaining follow-up colonoscopy after an abnormal fecal test could help deliver early interventions that increase colonoscopy adherence (e.g., patient navigation) to patients who need them most. We sought to develop a usable risk prediction model to identify patients unlikely to complete a colonoscopy following an abnormal FIT test.
Methods: We recalibrated and then redeveloped a prediction model created in a group of federally qualified health centers (FQHCs) to be used in a single large FQHC. The models were created from a retrospective cohort of patients aged 50-75 with an abnormal FIT test. The models used clinical data. Logistic and Cox regressions were used to recalibrate the group of FQHC prediction model and then redevelop it in the single large FQHC.
Results: The initial risk model used data from 8 FQHCs (26 clinics) and included eight variables including race, clinic system, prior missed appointments, insurance, prior flu shots, age, indication of anticoagulation use, and income inequality. The first model included 1723 patients. However, when we applied the model to a single large FQHC (34 clinics, n=884), the model did not recalibrate successfully (C-statistic dropped more than 0.05, from 0.66 to 0.61). The model was redeveloped in a cohort of 1401 patients and contained 12 variables including age, race, language, insurance, county, a composite variable for sex and mammogram screening, number of prior missed appointments, Gagne’s comorbidity score, number of prior encounters, BMI, marital status, and prior screening with a c-statistic of 0.65.
Conclusions: The original model developed in a group of FQHCs did not adequately recalibrate in the single large FQHC. Health system, patient or specialty care characteristics, or differences in data captured in the electronic health record may have led to the inability to recalibrate the model. However, the redeveloped model provides an adequate model for the single FQHC. Precision medicine is best applied when risk is understood in context and interventions are tailored for specific populations’ predictors.
Quality assessment of malaria microscopic diagnosis at the LPM-HALD of Dakar, Senegal, in 2020
Authors: GARBA Mamane Nassirou , DEME Awa Bineta , DIALLO Mamadou Alpha , DIEDHIOU Younous , MBAYE Amadou Mactar , DIA Mareme , SECK Anna , ZOUMAROU Daba , NDIAYE Yaye Die , NDIAYE Lamine , YADE Mamadou Samb , DIEYE Baba , SENE Aita , TINE Abdoulaye , TOURE Mariama , GADIAGA Nogaye , FALL Awa , NGOM Bassirou , SOW Djiby , NDIAYE Aliou , KEITA Astou , NDIAYE Mame Fama , GOMIS Jules François , DIOP Fatoumata , DIALLO Guete , NDIAYE Ibrahima Mbaye , BA Elhadj Meissa , BITEYE Omar , NDIAYE Cheikh , NDIAYE Pape Ousmane , GAYE Amy , SY Mouhamad , NDIAYE Tolla , DIONGUE Serigne Khadim , SECK Mame Cheikh , NDIAYE Mouhamadou , BADIANE Aida Sadikh , NDIAYE Daouda
Published: September 2022

Background: Following WHO guidelines, microscopy is the reference for malaria diagnosis in endemic countries. The Parasitology-Mycology laboratory (LPM) is the National Reference Laboratory and is currently undergoing ISO 15189 accreditation. In this perspective, we proceeded to laboratory verification through assessing the performance of the laboratory by confirming the reliability and the accuracy of the results obtained in accordance with the requirements of the ISO 15189 standards. This study aimed to verify the method of microscopic diagnosis of malaria at the LPM, in the Aristide Le Dantec hospital (HALD) in Dakar, Senegal.
Methods: This is a validation/verification study conducted from June to August 2020. Twenty (20) microscopic slides of thick/thin smear with known parasite densities (PD) selected from the Cheick Anta Diop University malaria slide bank in Dakar were used for this assessment. Six (6) were used to assess readers' ability to determine PD and fourteen (14) were used for detection and identification of parasites. Four (4) LPM-HALD microscopists read and recorded their results on prepared sheets. Data analysis was done with Microsoft Excel 2010 software.
Results: Of these twenty (20) slides read, a 100% agreement was obtained on eight (8) slides. Four (4) out of the six (6) parasite density evaluation slides obtained a concordance of less than 50%. Thirteen (13) out of the fourteen (14) identification slides obtained a concordance greater than 50%. Only one (1) identification slide obtained zero agreement from the readers. On the other hand, for species identification it was noted a score greater than 80% and the PD obtained a score between 0.20 and 0.4. Readers obtained 100% precision, sensitivity, specificity and both negative and positive predictive values.
Conclusion: This work demonstrated that the microscopic method of malaria diagnosis used in the LPM/HALD are in accoradance with all the reliability required by ISO 15189.
Workload Indicators of Staffing Need (WISN) Method for Midwives Planning and Estimation at Asrade Zewude MemorialPrimary Hospital, North west Ethiopia
Authors: Asres Gizew
Published: September 2022

Background: Work force is a crucial component of health service delivery system. Ethiopia faces health workforce challenges with regard to evidence based health work force planning. First the health worker to population ratio was used and later standard staffing schedule for each health facility level was used. Both of these methods did not address the issue of evidence based workload variation between the same levels of facilities at different locations within a country. A workload indicator of staffing need (WISN) method addresses these variations. Therefore this study was done to determine the gaps or excess and workload pressure in midwives, thereby to promote on the use of WISN for health facilities based on WISN results of midwives at Asrade Zewude memorial Hospital.
Methods: Cross sectional study using WISN model was used to determine the gaps or excess and workload pressure in midwives at Asrade Zewude Memorial primary hospital, North West Ethiopia. Midwives were selected based on priority point scale as outlined at WISN method.
Results: The finding showed that there were five working days each week with 1030 hours actual working time per year for midwives. This working time was spent on health service activities (58.4%), additional activities (36.6%) and support activities (5%). WISN calculation showed that a shortage of five midwives with WISN ratio of 0.8 at Asrade Zewude Memorial primary hospital North West Ethiopia.
Conclusion: Midwives at the study area were doing their routine work with 20% under staffed by covering the additional five midwives' position. With this working condition, it may be hard to achieve universal health coverage goals of the facility. Therefore the hospital should institutionalize WISN method planning to objectively employ midwifery professionals. This study had limitations too as it used retrospective annual service statistics and small sample size which affects generalization of the results to other health facilities and other health worker cadres within the study hospital.