bluuebunny/crossref_metadata_2025 huggingface dataset:
Gender Disparities in Dropping out of High School Students and their Level of Depression
Authors: Salim Airin , Sultana Rehana , Akhter Syeda Sharmina , Azim Sabrina Fahmida , Hoshen Md Minuddin , Islam Md Zakirul
Published: June 2023

Background: A drop-out can be defined as a child who enrols in school but fails to complete the relevant level of the educational cycle. It is usually associated with non-enrolment and a lower percentage of girls than boys in the school system. High school dropouts are more likely to be on welfare, have poorer health, have reduced intergenerational mobility, become teenage parents, unemployed, depressed, have substance abuse problems, engage in violent behaviours, and end up incarcerated. Adolescent depression is a mental and emotional disorder affecting adolescents and teens. According to WHO, globally depression is the number one cause of illness and disability in adolescent age group. Depression increases a teen’s risk for attempting suicide by 12 times and 30% of teens with depression also develop a substance abuse problem.

Objective: To find out the gender disparities in dropping out of high school students and their level of depression.

Methodology: This cross-sectional study was done in Adarsha Sadar, Cumilla, Bangladesh in between January 2014 to December 2014. Study sample was 339 adolescents of 13-19 years of that area. By purposive sampling technique data were collected through a pretested, semi-structed, interview questionnaire. Back Depression Inventory scale was used to assess the level of depression.

Results: Proportion of male drop out adolescent was 54% and female drop out adolescents 46%. 44.5% adolescents had depression and a positive association was found between adolescent depression and dropping out from high school.

Conclusions: There is an apparent gender gap among the high school dropouts, with more male adolescents dropping out than females. A positive association was found between the high school dropouts and the development of adolescent depression.

Eastern Med Coll J. Jan 2023; 8 (1), 1-5
Los retos de la integración andina
Authors:   Universidad Internacional del Ecuador, Altamirano-Flores Jorge , Tonon-Ordóñez Luis ,   Universidad del Azuay
Published: June 2023

Este volumen, fruto de una prolija investigación de equipos interdisciplinarios de UIDE y de la UDA. Describe los avances y retrocesos de la integración andina, sus relanzamientos, su reforma institucional, la modificación de algunos de sus objetivos iniciales y de su membresía original, los logros alcanzados y las tareas futuras, el importante acervo jurídico e institucional desarrollado desde 1969, el impacto de la globalización y de la tecnología en el desarrollo del proceso integracionista. Identifica, a su vez, a su proyecto más exitoso y destaca la tendencia a incluir, en los últimos años, áreas más complejas que trascienden lo comercial y lo económico.Se debe relievar la convicción de los autores y de los reconocidos expertos entrevistados que, a pesar de los cambios en el sistema internacional del último medio siglo que la han impactado, sigue la CAN siendo importante para el desarrollo de los países miembros. La publicación pasa revista a valiosos testimonios de notables profesionales y académicos ligados muy estrechamente, en diversos períodos, a la integración andina. Este aspecto constituye un acierto del trabajo al permitir que se difundan, en varios diálogos, importantes puntos de vista guiados por la experiencia acumulada. En francas y agudas reflexiones se esclarecen los hitos del proceso, los inevitables cambios que se produjeron, la consecución de algunos de sus objetivos originales y la viabilidad futura del proyecto integracionista andino. El libro incluye capítulos especializados dedicados al comercio intrasubregional andino y temas conexos que versan sobre el modelo de gravedad; el transporte y la logística; los productos de mayor comercialización entre Ecuador y los países de la CAN entre 1990 y 2019; el efecto del tipo de cambio real en las exportaciones ecuatorianas hacia Bolivia, Colombia y Perú, en el período 2003-2019, y dos capítulos analíticos y de reflexión del proceso y del regionalismo: un análisis retro y prospectivo a 50 años de la creación de la integración andina y un repaso del rompecabezas del regionalismo latinoamericano en un contexto de incertidumbres globales. 274 páginas Registro ISBN: 9 789942 618559 ISBN digital: 9 789942 618566
PENGARUH CITRA MEREK, KUALITAS PRODUK, DAN PERSEPSI HARGA TERHADAP KEPUTUSAN PEMBELIAN
Authors: Azahari Al Azahari , Hakim Lukmanul
Published: June 2023

Penelitian ini bertujuan untuk mengetahui: (1) pengaruh citra merek terhadap keputusan pembelian, (2) pengaruh kualitas produk terhadap keputusan pembelian, (3) pengaruh persepsi harga terhadap keputusan pembelian, (4) pengaruh citra merek, kualitas produk dan persepsi harga secara simultan terhadap keputusan pembelian. Penelitian ini merupakan penelitian asosiatif kausal. Populasi pada penelitian ini adalah konsumen Mobil Daihatsu Xenia di Kabupaten Sleman. Teknik pengambilan sampel menggunakan insidental sampling dengan jumlah sampel sebanyak 90 orang. Teknik pengumpulan data menggunakan kuesioner yang telah diujikan validitas dan reliabilitas. Teknik analisis data yang digunakan adalah regresi berganda. Hasil penelitian menunjukkan bahwa: (1) citra merek berpengaruh negatif terhadap keputusan pembelian dengan tingkat signifikansi (2) kualitas produk berpengaruh positif terhadap keputusan pembelian. (3) persepsi harga berpengaruh positif terhadap keputusan pembelian (4) citra merek, kualitas produk dan persepsi harga secara simultan memiliki pengaruh positif terhadap keputusan pembelian.
PENGARUH GAYA KEPEMIMPINAN, BUDAYA ORGANISASI, MOTIVASI, DAN LINGKUNGAN KERJA TERHADAP KINERJA PEGAWAI
Authors: Ratnasari Sri Langgeng , Sutjahjo Gandhi
Published: June 2023

Penelitian ini merupakan penelitian kuantitatif, dengan tujuan untuk menganalisis pengaruh Gaya Kepemimpinan, Budaya Organisasi, Motivasi dan Lingkungan Kerja terhadap Kinerja Pegawai. Populasi penelitian adalah 87 pegawai BMG Kota Batam. Penelitian dilakukan dengan menggunakan metode sensus, dimana responden sebanyak 87 pegawai. Analisis yang pada penelitian ini menggunakan metode analisis regresi linier berganda. Berdasarkan hasil pengujian sebagai berikut, (1). Gaya kepemimpinan berpengaruh signifikan terhadap kinerja pegawai (2). Budaya organisasi berpengaruh signifikan terhadap kinerja pegawai (3) Motivasi berpengaruh signifikan terhadap kinerja pegawai (4) Lingkungan kerja berpengaruh signifikan terhadap kinerja pegawai (5) Gaya kepemimpinan, budaya organisasi, motivasi dan lingkungan kerja secara bersama-sama berpengaruh signifikan terhadap kinerja pegawai.
PENGARUH BRAND IMAGE, HARGA DAN KUALITAS PRODUK TERHADAP KEPUTUSAN PEMBELIAN
Authors: Irwan Irwan , Wibowo Edwin Agung
Published: June 2023

Penelitian ini bertujuan untuk menguji dan menganalisis pengaruh citra merek, harga dan kualitas produk terhadap keputusan pembelian ban forklift merek annair kota Batam. Pengumpulan data dilakukan melalui kuesioner dan dilakukan dengan menggunakan SPSS versi 16. Teknik pengambilan sampel yang digunakan adalah metode sensus dan teknik dan teknik uji data yang digunakan dalam penelitian ini meliputi analisis faktor, uji validitas, uji reliabilitas dengan Cronbach Alpha. Uji asumsi klasik dan analisis regresi linier berganda, untuk menguji dan membuktikan hipotesis penelitian. Pengujian hipotesis dengan menggunakan uji t menunjukkan bahwa variabel citra merek berpengaruh tidak signifikan terhadap keputusan pembelian, harga berpengaruh tidak signifikan terhadap keputusan pembelian, kualitas produk bepengaruh tidak signifikan terhadap keputusan pembelian. Kemudian melalui uji F dapat diketahui bahwa ketiga variabel bebas secara simultan berpengaruh tidak signifikan terhadap keputusan pembelian.
PENGENALAN LAYANAN APLIKASI JAMSOSTEK MOBILE MELALUI VIDEO PROMOSI DAN BANNER PADA BPJS KETENAGAKERJAAN CABANG MAKASSAR
Authors: Sampe Pande Jenica , Gunawan Maylisa
Published: June 2023

Jamsostek Mobile merupakan suatu aplikasi terbaru dari BP-Jamsostek yang memberikan layanan kepada para pesertanya melalui online. JMO memiliki fitur-fitur yang lebih lengkap disbanding dengan aplikasi sebelumnya seperti fitur klaim, pelaporan kecelakaan kerja, co-marketing, serta jaringan mitra layanan dan kantor cabang. Tujuan penelitian ini adalah untuk membuat strategi promosi menggunakan media iklan yaitu video dan banner dalam rangka mensosialisasikan aplikasi JMO serta merumuskan dampak yang ditimbulkan dari promosi Jamsostek Mobile (JMO) menggunakan media iklan terhadap peserta BPJS Ketenagakerjaan. Hasil penelitian diperoleh data bahwa sebagian masyarakat lebih tertarik melihat video promosi di media sosial dari pada melalui media cetak. Hal ini dikarenakan video promosi sifatnya lebih padat, jelas dan mudah dimengerti serta penyebaran informasinya pun lebih cepat dalam menjangkau masyarakat. Sehingga promosi melalui media video dapat cepat dalam meningkatkan jumlah konsumen atau pengguna suatu produk dan jasa pada suatu perusahaan. Adapun strategi promosi lain yang digunakan adalah banner. Hasil penelitian menunjukkan bahwa sebagian masyarakat tertarik melihat promosi melalui banner. Hal ini dikarenakan banner biasanya ditempatkan pada tempat umum yang dapat dijangkau semua orang dan penyebaran informasinya sangat cepat. Sehingga promosi melalui banner efektif digunakan dalam memperkenalkan produk atau jasa.
STANDAR OPERASIONAL PROSEDUR (SOP) PADA UNIT HR&GA PT BOSOWA PROPERTINDO MAKASSAR
Authors: Sampe Valentin Laura , Fauzi Muflih Irsyad
Published: June 2023

Standar operasional prosedur (SOP) menjadi sebuah pedoman serta dasar dalam mengerjakan suatu pekerjaan dengan cara yang paling efisien dan efektif dengan memperhatikan beberapa faktor seperti keselamatan kerja, keamanan bekerja dan ketepatan dalam bekerja hal ini berfungsi untuk meningkatkan kinerja perusahaan PT Bosowa Propertindo Makassar dikarenakan SOP yang sebelumnya yang ada di PT Bosowa Propertindo belum dilakukan pembaharuan sehingga membuat SOP sebelumnya sudah tidak lagi sesuai dengan aturan-aturan perusahaan yang terbaru serta penggunaan sistem baru yang mengharuskan adanya pembaharuan SOP dan ada beberapa SOP yang belum ada sebelumnya, tujuan dari penelitian ini untuk menghasilkan SOP guna mengoptimalkan kinerja Unit Human Resource and General Affair (HR&GA). Metode penelitian yang digunakan adalah metode kualitatif dengan melakukan wawancara dengan pihak yang bersentuhan langsung dengan pekerjaan tersebut untuk mengetahui atau menentukan prosedur yang paling efektif dan efisien dalam mengerjakan pekerjaan tersebut yang akan dimasukkan dalam SOP yang akan dibuat serta melakukan telaah dokumen guna memvalidasi semua sumber data yang dibutuhkan agar tidak adanya kekeliruan yang fatal yang dapat membuat SOP ini tidak berjalan secara maksimal. Output dari penelitian ini terdiri dari lima SOP yaitu SOP Rekrutmen, SOP Penggajian, SOP pendaftaran dan pemberhentian BPJS kesehatan, SOP pengadaan inventaris dan terakhir SOP pengajuan cuti menggunakan HRIS.
PENGEMBANGAN KEMASAN DENGAN MENGGUNAKAN APLIKASI CANVA PADA UMKM KOPI 919
Authors: Rahmat Rahmat , Anastasia Anastasia
Published: June 2023

Dunia bisnis akan selalu dihadapkan dengan perubahan, ini disebabkan oleh banyak faktor seperti tingginya daya saing antar perusahaan dan tingginya kemauan masyarakat akan perubahan produk agar bisa semakin praktis untuk digunakan.. Sebuah perusahaan harus bisa mengikuti perkembangan zaman, salah satu caranya yaitu dengan melakukan pengembangan kemasan. UMKM Kopi 919 adalah sebuah bisnis yang terletak di Perumahan Buludatu Blok B No.5. Kemasan yang digunakan  UMKM 919 masih sangat kuno dan terkesan ketinggalan zaman sehingga peneliti akan mengembangkan kemasannya yang meliputi 5 aspek penting yaitu bentuk kemasan, bahan kemasan, warna, font dan merek. Tujuan dari penelitian ini adalah untuk mengetahui proses tahapan pengembangan produk pada Kopi 919 dan juga menciptakan kemasan kopi yang sedang trend dengan menggunakan aplikasi canva. Penelitian ini dilakukan selama kurang lebih empat bulan yaitu dari bulan April 2022 hingga Agustus 2022 dan melalui 3 tahap prosedur penelitian yaitu penciptaan ide kemasan, penyaringan ide dan pengembangan kemasan. Penelitian ini menggunakan jenis data berupa data kualitatif. Teknik pengumpulan data dalam penelitian ini dilakukan dengan observasi (pengamatan), interview (wawancara) dan dokumentasi. Hasil penelitian menunjukkan bahwa standing pouch berbahan aluminium foil adalah kemasan yang cocok untuk kopi bubuk dan disukai oleh masyarakat. Selain itu, masa penyimpanan, logo/merek, berat bersih dan komposisi adalah informasi yang dibutuhkan masyarakat serta warna yang cocok untuk produk kopi bubuk adalah coklat dan hitam.
PENGEMBANGAN STANDAR OPERASIONAL PROSEDUR PADA BIDANG PEMBINAAN PEMERINTAHAN DESA KABUPATEN TAKALAR
Authors: Rahman Aulia , Fadillah Nur Dwiyanti Dhifara
Published: June 2023

Berdasarkan peraturan yang ada, setiap organisasi pemerintah diharuskan mempunyai standar pelayanan, salah satunya adalah SOP. Salah satu manfaat SOP adalah untuk meningkatkan kinerja pemerintah agar lebih efektif dan efisien. Hasil wawancara pra penelitian ternyata menemukan bahwa ada beberapa SOP di Dinas Sosial dan PMD Kabupaten Takalar khususnya Bidang Pembinaan Pemerintahan Desa yang perlu diperbaharui, sehingga tujuan penelitian ini adalah untuk mengembangkan atau memperbaharui Standar Operasional Prosedur (SOP) khususnya pada Bidang Pembinaan Pemerintahan Desa Dinas Sosial Pemberdayaan Masyarakat dan Desa Kabupaten Takalar. Metode Penelitian yang digunakan, adalah metode kualitatif dengan desain penelitian deskriptif. Teknik pengumpulan data dalam penelitian ini mengguanakan wawancara, observasi dan telaah dokumen. Sedangkan analisis data menggunakan (1) reduksi data, (2) penyajian data dan (3) penarikan kesimpulan. Kemudian untuk uji keabsahan data, penelitian ini mengggunakan uji keabsahan data dengan trianggulasi sumber. Output atau hasil dari penelitian ini adalah, SOP Supervisi dan Asistensi Pengelolaan Keuangan dan Aset Desa, SOP Pembinaan Pengelolaan Keuangan dan Aset Desa, SOP Verifikasi dan Pelaporan Pelaksanaan Alokasi Dana Desa, dan SOP Penyusunan dan Pembinaan Sistem Informasi Keuangan Desa.
A Study on Investment Determinants of Webtoon Content Companies
Authors:   The Korean Society of Culture and Convergence, Kim Ahhyeon , Kim Sun Young
Published: June 2023

This study attempted to provide ideas in establishing investment strategies by analyzing the determinants of investment in domestic webtoon company. The Delphi survey method was adopted targeting investment entities of various webtoon companies. As a result of the analysis, major investment determinants considering the characteristics of the industry were the current status of securing webtoon IP, the possibility of IP expansion, flexible thinking and execution and the competence and recognition of corporate-owned artist. On the other hand, it was found that the rationality of distribution of the industry, which includes illegal distribution of webtoon, was not considered in investment determinants. These findings suggest that company should have the ability to produce high-quality content that can expand IP based on the establishment of a creative environment to attract investment. This study is meaningful in that it presented a direction to establish an environment to revitalize investment in the webtoon industry by analyzing the determinants of investment decisions in webtoon content company.
The Relationship between Self-Discrepancy and Trait-Anxiety in College Students: Mediating Effects of Dispositional Self-focused Attention and Maladaptive Cognitive Emotion Regulation Strategies
Authors:   The Korean Society of Culture and Convergence, Choi Sunah , Kim Sung Bong , Kim Woonok
Published: June 2023

This study aimed to verify the independent and sequential mediating effect of dispositional self-focused attention and maladaptive cognitive emotion regulation strategies in the relationship between self-discrepancy and trait-anxiety in college students. For this, data was collected about Self-Questionnaire, State Trait Anxiety Inventory-Y(STAI-Y), Scale for Dispositional Self-focused Attention in Social situation(SDSAS) and Cognitive Emotion Regulation Questionnaire(K-CERQ) from 400 college students, and the Structural Equation Model analysis was conducted on the collected data using SPSS 22.0 and AMOS 22.0. As a result of this analysis, it was confirmed that dispositional self-focused attention and maladaptive cognitive emotion regulation strategies had significant independent and sequential mediating effects in the relationship between self-discrepancy and trait-anxiety. This study is significant in that it comprehensively confirmed the role of emotion regulation, which mediates the process of self-discrepancy leading to trait-anxiety, by dividing it into the dimension of emotional recognition and correction.
Gender-Based Violence in Bosnia From Conflict to The Post-Conflict Setting
Authors: KURAN İbrahim
Published: June 2023

This review article aims to investigate gender-based violence in wartime and post-war Bosnia. While the existing literature exclusively focuses on the relationship between gender and nationalism, this article rather addresses the psychological and economic violence against women in Bosnia. By adopting a continuum approach, this study uncovers how different forms of violence against women interact with each other from the conflict to the post-conflict contexts. Through a literature survey, this study depicts how gender-based violence has been reproduced from past to present in the case of Bosnia. In discussing gender-based violence, it dwells upon the intersections between mass rapes, forced pregnancy and traumatization. As the main argument, this article contends that sexual violence during the war triggered the spiral of violence and further led to stigmatization and marginalization of these women in the post-war context. It is especially argued that the persistent public stigma is the most blatant manifestation of psychological violence against female rape victims. In addition, it is shown that the overlapping processes of conflict, patriarchy and neoliberalization have led to a series of economic injustices, such as, in the form of the dispossession, poverty and exclusion of women in Bosnia. This article overall suggests establishing linkages between sexual, psychological and economic violence against women during the conflict and post-conflict processes.
CONCEPT OF RETRACTABLE CONSENT IN MINISTER OF EDUCATION’S REGULATION REGARDING SEXUAL VIOLENCE ON CAMPUS
Authors: Lazuardi Adi , Pribadi Muhammad Akbar
Published: June 2023

The Regulation of Minister of Education, Culture, Research and Technology (Permendikbud) 30/2021 is a response to the high level of sexual violence case perpetrated by individuals in universities in Indonesia. The existence of a victim's consent instrument in Permendikbud 30/2021 as a differentiator between sexual violence and a consensual relationship is a new thing in legal regulation in Indonesia. This research aims to explain the concept of retractable consent and its application in Permendikbud 30/2021. This study uses a descriptive analysis method to data sources on regulations that are considered relevant to the agreement of the parties in general, and regulations related to sexual violence in particular. The results show that retractable consent in cases of sexual violence is the consent of one of the parties involved in sexual activities that can be withdrawn or canceled at any time, even when sexual activity has started. In this concept, the agreement has the nature of sustainability. Permendikbud 30/2021 regulates the invalidity of consent with certain conditions, so that the consent given by one of the parties in a sexual activity can be considered legally invalid. According to Permendikbud 30/2021 victims of sexual violence who apply personal consent retraction (cancellation or withdrawal of consent for personal reasons) should also be included in the victim category.
LEGAL ASSISTANCE FOR THE POOR TO REACH JUSTICE
Authors: Arliman S Laurensius , Suryanef Suryanef , Arif Ernita , Sarmiati Sarmiati
Published: June 2023

Legal aid to the poor has a fairly important position in every criminal, civil and administrative justice system, including Indonesia. The concept of legal aid to the poor is related to a person's rights to exercise these rights, therefore legal aid is carried out by legal experts and experienced people in order to carry out their profession. The purpose of this study is to explain legal aid for the poor to achieve justice. This research uses normative legal research which is described descriptively. The results of the study explain that legal protection is an action or effort to protect the community from arbitrary actions by authorities that are not in accordance with the rule of law, to create order and peace, thus enabling humans to enjoy their dignity as human beings. There are ten rules related to legal aid to the poor, both at the constitutional level to the law, Internal Regulations that apply to the Supreme Court and Internal Regulations that apply to the Ministry of Law and Human Rights, as a pattern and legal standing for the provision of legal aid for the poor. poor people in Indonesia. The provision of legal aid to the poor is a form of justice for the poor. The concept of legal aid to the poor is related to a person's rights to exercise their rights under the same law.
THE EXONERATION CLAUSES IN PARKING PRACTICES: A REVIEW FROM THE PRINCIPLE OF PROPORTIONALITY AND INCLUSIVE LAW
Authors: Simbolon Herbert Petrus Wiro , Kurniawan I Gede Agus
Published: June 2023

This research focuses on the analysis of exoneration clauses in parking practices from the perspective of proportionality and inclusive law perspectives. This research is a normative legal research with a concept, case, and statutory approach. The results of the study confirm that exoneration clauses in parking practices are usually not in accordance with the principle of proportionality because they ignore the substance in various court decisions where one of the substances is the exoneration clause should not be a means to "escape" from the responsibility of the parking manager because as stated in MA Decision No. 3416/Pdt/1985 parking practices are constructed as goods safekeeping agreements and the existence of an exoneration clause does not eliminate the responsibility of the parking manager to safeguard the goods deposited as long as it can be proven without any intention or negligence by the parking manager. Therefore, in the future the principle of proportionality can serve as a guide as well as a tester for the substance of the exoneration clause in parking practices. Furthermore, an inclusive legal perspective with regard to exoneration clauses in parking practices, in fact, exoneration clauses in parking practices is contrary to the spirit of prophetic law which emphasizes balanced legal relations and relations between parking managers and parking users as consumers. For this reason, to overcome the existence of an exoneration clause in parking practices is to involve the role of the regions in formulating a Regional Regulation which states that the exoneration clause in parking practices with the substance of business actors, namely the parking manager, is not responsible for losses suffered by consumers, namely parking users, is null and void by law. and can be accompanied as in the provisions of the PK Law.
COMPARATIVE ANALYSIS OF COCOA CERTIFICATION AND NON-CERTIFICATION FARMING IN KELAPA DUA VILLAGE, ANREAPI DISTRICT, POLEWALI MANDAR REGENCY
Authors: Muh. Arman Yamin Pagala  , Megawati
Published: June 2023

Cocoa is one of the plantation crops that is widely cultivated in Polewali Mandar, the cocoa certification program is an effort to increase cocoa production and prices at the farm level. This study aims to determine the comparison of the income level of cocoa farms through the cocoa certification program and farms that have never participated in the certification program while developing the cocoa commodity, as well as to determine the comparison of R/C ratio and B/C ratio values. The implementation of this research began in May to July 2021, in Kelapa Dua village. Data collection techniques used structured interviews using questionnaires and question and answer. The results showed that the average income achieved by certified cocoa farmers is Rs 5,572.75 per/ha. When compared to the average income of non-certified cocoa farmers is Rs 4,715,429 per/ha. The difference between the income of certified and non-certified cocoa farmers is Rp. 857,325. The results of the R/C ratio analysis for certified cocoa can be planted by looking at the R/C ratio value of 2.40 and for non-certified cocoa has an R/C ratio value of 2.27. Profit B/C ratio result as a result of certified and non-certified income is 3.12. This shows that both certified and non-certified cocoa are feasible to develop, but certified cocoa has a greater income than non-certified cocoa.
Dampak Lama Penyimpanan Arsip Sampel Formalin-Fixed Paraffin-Embedded (FFPE) dengan Konsentrasi Ribonucleid acid (RNA) yang Diekstraksi Menggunakan Kit Komersial
Authors: Wiraditya Nur Eka
Published: June 2023

Formalin fixation and paraffin embedding (FFPE) merupakan salah satu metode penyimpananrsip sampel dalam jangka waktu bertahun-tahun. Penyimpanan sampel dalam jangka waktu yang lama memungkinkan terjadinya penurunan kualitas RNA pada sampel. Penelitian iniengetahui perbedaan konsentrasi RNA pada arsip sampel 2019 dan 2020 dengan satuan ng/L. Penelitian menggunakan sampel yang diambil dari arsip FFPE Rumah Sakit (RS) X sebanyak 20 sampel. Rincian sampel adalah 10 (sepuluh) sampel pada tahun 2019 dan 10 (sepuluh) sampel pada tahun 2020. Penelitian ini dilakukan pada tahun 2021 di laboratorium Patologi Anatomik FK-KMK UGM. Penelitian ini menunjukkan dampak signifikan terhadap lama penyimpanan arsip sampel FFPE ditunjukkan dengan penurunan konsentrasi RNA antara tahun 2019 dan 2020 dengan perbedaan konsentrasi rata-rata 12,3 ng/L.
Keragaman Spesies Satwa Liar Berbasis Bidikan Kamera Jebak (Camera Trap) di Cagar Alam Pulau Sempu, Kab. Malang, Jawa Timur
Authors: Makmum Mohamad Sukron
Published: June 2023

Kekhawatiran dan kerentanan akan terjadinya kerusakan eksosistem di Cagar Alam Pulau Sempu (CAPS), Kabupaten Malang oleh aktivitas manusia dapat berdampak pada kelangsungan kehidupan dan penurunan populasi satwa liar. Penelitian bertujuan untuk menghitung kekayaan spesies dan frekuensi perjumpaan spesies satwa liar dengan menggunakan kamera jebak di kawasan CAPS. Metodologi menggunakan penghitungan bukti rekaman foto digital kamera jebak. Satwa liar yang tertangkap pada kamera jebak dihitung berdasarkan jumlah foto, jumlah spesies, dan jumlah hari aktif kamera. Hasil penelitian menunjukkan kekayaan satwa liar yang teridentifikasi sebanyak 15 spesies, yaitu Muntiacus muntjak (Cervidae), Macaca fascicularis (Cercopithecidae), Paradoxurus hermaprodithus (Viverridae), Callosciurus notatus (Sciuridae), Tragulus javanicus (Tragulidae), Hystrix javanica (Hystricidae), Trachypithecus auratus (Cercopithecidae), Sus scrofa (Suidae), Prionodon linsang (Prionodontidae), Varanus nebulosus (Varanidae), Gallus gallus (Phasianidae), Chalcophaps indica (Columbidae), Spilornis cheela (Accipitridae), Hydrornis guajanus (Pittidae) dan Varanus salvator (Varanidae). Hasil penghitungan nilai frekuensi perjumpaan satwa liar relatif bervarisi dari nilai yang tinggi sampai rendah, namun dari 15 spesies yang tercatat perolehan tertinggi didominasi oleh Muntiacus muntjak (ER 11,17 foto/hari) dan Macaca fascicularis (ER 10 foto/hari).
ANALISIS PENCEMARAN LOGAM BERAT PADA SEDIMEN PERMUKAAN DI DASAR PERAIRAN LAUT PULAU PASARAN
Authors: Hamid Abdul , Riani Etty , Warlina Lina
Published: June 2023

Kawasan muara merupakan salah satu ekosistem perairan yang sering mengalami kontaminasi logam berat seperti nikel (Ni), cadmium (Cd), timbal (Pb), dan merkuri (Hg). Kontaminasi tersebut biasanya berasal dari aktivitas manusia seperti kegiatan rumah tangga, aktivitas perkapalan, dan kegiatan industri. Salah satu wilayah perairan yang terletak di sekitar kawasan muara adalah perairan laut di sekitar Pulau Pasaran. Kawasan ini terkenal sebagai salah satu sentra pembuatan ikan asin di Provinsi Lampung, Indonesia. Kawasan tersebut juga digunakan sebagai tempat penangkapan dan budi daya sumber daya kelautan seperti ikan dan kerang. Penelitian ini dilakukan dengan tujuan untuk menganalisis tingkat pencemaran logam berat jenis Ni, Cd, Pb, dan Hg yang terdapat pada sedimen permukaan di dasar laut Pulau Pasaran. Metode penelitian ini menggunakan pendekatan deskriptif kuantitatif dengan menganalisis tingkat pencemaran menggunakan Geoaccumulation Index (Igeo). Hasil penelitian menunjukkan bahwa sedimen permukaan di dasar laut Pulau Pasaran tidak terindikasi mengalami pencemaran Ni, Cd, dan Hg. Meski demikian, hasil penghitungan Igeo mengindikasikan adanya kontaminasi Pb dengan tingkat pencemaran berat hingga tercemar sangat berat.
A ocupação de Guarapuava: desigualdades socioespaciais e educacionais a partir da perspectiva decolonial latino-americana
Authors: Lara Viviane de , Barbosa Mônica Matos , Silva Jefferson Olivatto da
Published: June 2023

A partir da perspectiva decolonial, o presente estudo é composto de reflexões teóricas sobre a influência do colonialismo na produção de desigualdades sociais e, consequentemente, educacionais, no espaço urbano da cidade de Guarapuava, no Paraná, e sobretudo as produzidas em torno da ideia de “raça”, conceito empregado para diferenciar sujeitos e hierarquizar as relações sociais. Para tanto, partimos da análise das origens de Guarapuava, discorrendo sobre seu modelo constituído por urbanização e territorialização, concepções, conceitos, planos e práticas de produção e uso dos espaços. Por meio dos aspectos históricos, despertamos o entendimento acerca das desigualdades consolidadas na sociedade latino-americana de que o fenômeno urbano é um acúmulo de bens culturais, assim o resultado que temos é cumulativo de todos os acontecimentos históricos, visto que a urbanização é uma das tendências globais mais nítidas e que, no decorrer do tempo, vai adquirindo determinadas especificidades.
Crimigração: a fronteira institucional de intersecção entre a política migratória e a política criminal – o caso da União Europeia e Estados Unidos da América
Authors: Santos Josuel Belo dos , Preussler Gustavo de Souza
Published: June 2023

Denomina-se “crimigração” a postura pragmática estatal que se utiliza da lei penal em convergência com a de migração, com objetivo de criminalizar as migrações e punir os imigrantes, criando-se, dessa forma, uma fronteira institucional, conjugada ao paradoxo do etiquetamento social e securitização das fronteiras. Para tal, muitos Estados optam pela adoção de agendas crimigratórias avessas aos tratados internacionais de proteção do migrante e empregam o controle social, tipificando-o na condição jurídica de irregular ou ilegal. O presente estudo desenvolveu-se por intermédio de pesquisa bibliográfica aliada à análise de dados. Buscou-se investigar tais fenômenos do processo da criminologia da mobilidade implementados pelo governo norte americano e alguns países do bloco europeu. Constata-se, no governo do ex-Presidente Trump, o maior declínio de entrada dos estrangeiros na história dos Estados Unidos (-87%). Ainda verifica-se que 736.900 deles foram expulsos da União Europeia entre 2020 e 2021.
Expressões econômicas da feira-livre: perfil e perspectiva dos feirantes
Authors: Pereira Viviane Guimarães , Pereira Miguel de Souza , Brito Tayrine Parreira , Goulart Ana Luiza Vieira , Pereira Samanta Borges
Published: June 2023

As feiras-livres são importantes canais de comercialização, configurando-se como um espaço de integração cultural e socioeconômica. Enquanto circuitos curtos de negociação, elas aproximam produtor e consumidor, fortalecendo vínculos e dinamizando a economia. Esta pesquisa objetivou analisar a expressão econômica da feira-livre em um bairro de Itajubá, sul de Minas Gerais, a partir do perfil e da perspectiva dos feirantes. Para tanto, foram entrevistados 56 feirantes, entre maio e dezembro de 2019, por meio de roteiros semiestruturados, divididos em duas partes fundamentais: (i) caracterização geral da feira e (ii) caracterização dos feirantes. Os resultados mostraram que a maioria dos feirantes comercializam sua própria produção e, de forma estratégica, complementam sua oferta adquirindo produtos constantemente demandados por frequentadores. A renda da feira se mostrou fundamental para a composição do faturamento dos empreendedores. Enfim, esta pesquisa pode subsidiar ações do poder público para o fortalecimento de outras feiras do município contemplado e região.
Para além da fronteira geográfica: encontro de culturas no Vale do Guaporé entre Brasil e Bolívia
Authors: Pereira Wilma Suely Batista , Oliveira Clebson Carlos de
Published: June 2023

A presente pesquisa de campo tem cunho exploratório e tem como objetivo analisar o encontro de culturas na fronteira guaporeana entre brasileiros e bolivianos. A coleta de dados foi feita através de observa-ções e os resultados evidenciam o encontro da diversidade e a influência boliviana no território rondoniense. Essas trocas permanentes de conhecimento são antigas e se iniciaram no período de povoamento da região, a partir do século XVIII. A diversidade de povos que ali se estabeleceram formando uma união geográfica e criaram raízes em comum, desse modo, é vista e sentida até os dias atuais entre os países.
Dual CSF1R inhibition and CD40 activation demonstrates anti-tumor activity in a 3D macrophage- HER2+ breast cancer spheroid model
Authors: Rodriguez-Perdigon Manuel , Haeni Laetitia , Rothen-Rutishauser Barbara , Rüegg Curzio
Published: June 2023

The complex interaction between tumor-associated macrophages (TAMs) and tumor cells through soluble factors provides essential cues for breast cancer progression. TAMs-targeted therapies have shown promising clinical therapeutical potential against cancer progression. The molecular mechanisms underlying the response to TAMs-targeted therapies depends on complex dynamics of immune cross-talk and its understanding is still incomplete. In vitro models are helpful to decipher complex responses to combined immunotherapies. In this study, we established and characterized a 3D human macrophage-ER+ PR+ HER2+ breast cancer model, referred to as macrophage-tumor spheroid (MTS). Macrophages integrated within the MTS had a mixed M2/M1 phenotype, abrogated the anti-proliferative effect of trastuzumab on tumor cells, and responded to IFNγ with increased M1-like polarization. The targeted treatment of MTS with a combined CSF1R kinase inhibitor and an activating anti-CD40 antibody increased M2 over M1 phenotype (CD163+/CD86+ and CD206+/CD86+ ratio) in time, abrogated G2/M cell cycle phase transition of cancer cells, promoted the secretion of TNF-α and reduced cancer cell viability. In comparison, combined treatment in a 2D macrophage-cancer cell co-culture model reduced M2 over M1 phenotype and decreased cancer cell viability. Our work shows that this MTS model is responsive to TAMs-targeted therapies, and may be used to study the response of ER+ PR+ HER2+ breast cancer lines to novel TAM-targeting therapies.
Effect of eye rubbing on corneal biomechanical properties in myopia and emmetropia
Authors: Li Xia , Wei Anji , Yang Yujing , Hong Jiaxu , Xu Jianjiang
Published: June 2023

Purpose: To investigate short-term changes in corneal biomechanical properties caused by eye rubbing in myopia and emmetropia and compare the different responses between the two groups.

Methods: This was a prospective observational study of 57 eyes of 57 healthy subjects aged 45 years and younger. The participants were divided into myopia and emmetropia groups. All the subjects underwent eye rubbing by the same investigator using the same technique. Biomechanical parameters were recorded using the Corvis ST device before and after 1 min of eye rubbing. One week later, all the participants underwent the test again. Statistical methods were employed to compare the differences between the data from before and after the 1 min of eye rubbing and demonstrate the different responses of the two groups.

Results: After 1 min of eye rubbing, smaller SP-A1 (p < 0.001), higher deformation and deflection amplitudes (p < 0.001, p = 0.012), higher peak distances (p < 0.001), earlier A1 times (p < 0.001), faster velocities (p < 0.001), and lower maximum inverse radii (p = 0.004) were observed. According to the automatic linear modeling analysis, the refractive states (B = −5.236, p = 0.010) and biomechanically corrected intraocular pressure (bIOP) (B = 0.196, p = 0.016) had influenced a decrease in the stiffness parameter at the first applanation (SP-A1). The central corneal thickness (CCT) had decreased only in the myopia group (p = 0.039). The change of SP-A1 in amplitude was larger in the myopia group than in the emmetropia group (p < 0.001). All the parameters returned to the baseline level 1 week later.

Conclusion: Eye rubbing appears to alter corneal biomechanical properties temporarily and make the cornea softer, especially for myopic young patients.
Micronutrient optimization for tissue engineered articular cartilage production of type II collagen
Authors: Cruz Maria A. , Gonzalez Yamilet , Vélez Toro Javier A. , Karimzadeh Makan , Rubbo Anthony , Morris Lauren , Medam Ramapaada , Splawn Taylor , Archer Marilyn , Fernandes Russell J. , Dennis James E. , Kean Thomas J.
Published: June 2023

Tissue Engineering of cartilage has been hampered by the inability of engineered tissue to express native levels of type II collagen in vitro. Inadequate levels of type II collagen are, in part, due to a failure to recapitulate the physiological environment in culture. In this study, we engineered primary rabbit chondrocytes to express a secreted reporter, Gaussia Luciferase, driven by the type II collagen promoter, and applied a Design of Experiments approach to assess chondrogenic differentiation in micronutrient-supplemented medium. Using a Response Surface Model, 240 combinations of micronutrients absent in standard chondrogenic differentiation medium, were screened and assessed for type II collagen promoter-driven Gaussia luciferase expression. While the target of this study was to establish a combination of all micronutrients, alpha-linolenic acid, copper, cobalt, chromium, manganese, molybdenum, vitamins A, E, D and B7 were all found to have a significant effect on type II collagen promoter activity. Five conditions containing all micronutrients predicted to produce the greatest luciferase expression were selected for further study. Validation of these conditions in 3D aggregates identified an optimal condition for type II collagen promoter activity. Engineered cartilage grown in this condition, showed a 170% increase in type II collagen expression (Day 22 Luminescence) and in Young’s tensile modulus compared to engineered cartilage in basal media alone.Collagen cross-linking analysis confirmed formation of type II-type II collagen and type II-type IX collagen cross-linked heteropolymeric fibrils, characteristic of mature native cartilage. Combining a Design of Experiments approach and secreted reporter cells in 3D aggregate culture enabled a high-throughput platform that can be used to identify more optimal physiological culture parameters for chondrogenesis.
Apoptosis-targeted gene therapy for non-small cell lung cancer using chitosan-poly-lactic-co-glycolic acid -based nano-delivery system and CASP8 and miRs 29A-B1 and 34A
Authors: Chattopadhyay Sourav , Sarkar Shashanka Shekhar , Saproo Sheetanshu , Yadav Sheetal , Antil Deepika , Das Bodhisatwa , Naidu Srivatsava
Published: June 2023

Non-small cell lung cancer (NSCLC) is a leading cause of cancer-related deaths worldwide, with resistance to apoptosis being a major driver of therapeutic resistance and aggressive phenotype. This study aimed to develop a novel gene therapy approach for NSCLC by targeting resistance to apoptosis. Loss of function mutations of caspase 8 (CASP8) and downregulation of microRNAs (miRs) 29A-B1 and 34A were identified as key contributors to resistance to apoptosis in NSCLC. A biodegradable polymeric nano-gene delivery system composed of chitosan-poly-lactic-co-glycolic acid was formulated to deliver initiator CASP8 and miRs 29A-B1 and 34A. The nano-formulation efficiently encapsulated the therapeutic genes effectively internalized into NSCLC cells and induced significant apoptosis. Evaluation of the nano-formulation in A549 tumor spheroids showed a significant increase in apoptosis within the core of the spheroids, suggesting effective penetration into the spheroid structures. We provide a novel nano-formulation that demonstrate therapeutic potential for suicidal gene therapy in NSCLC.
Yolk@Wrinkled-double shell smart nanoreactors: new platforms for mineralization of pharmaceutical wastewater
Authors: Habibi Zare Masoud , Mehrabani-Zeinabad Arjomand
Published: June 2023

Nanomaterials with “yolk and shell” “structure” can be considered as “nanoreactors” that have significant potential for application in catalysis. Especially in terms of electrochemical energy storage and conversion, the nanoelectrode has a large specific surface area with a unique yolk@shell structure, which can reduce the volume change of the electrode during the charging and discharging process and fast ion/electron transfer channels. The adsorption of products and the improvement of conversion reaction efficiency can greatly improve the stability, speed and cycle performance of the electrode, and it is a kind of ideal electrode material. In this research, heterojunction nanoreactors (FZT Y@WDS) Fe3O4@ZrO2-X@TiO2-X were firstly synthesized based on the solvothermal combined hard-template process, partial etching and calcination. The response surface method was used to determine the performance of the FZT Y@WDS heterojunction nanoreactors and the effects of four process factors: naproxen concentration (NAP), solution pH, the amount of charged photocatalyst, and the irradiation time for photocatalytic degradation of NAP under visible light irradiation. To maximize the photocatalytic activity, the parameters of the loaded catalyst, the pH of the reaction medium, the initial concentration of NAP, and the irradiation time were set to 0.5 g/L, 3, 10 mg/L, and 60 min, respectively, resulting in complete removal of NAP and the optimum amount was calculated to be 0.5 g/L, 5.246, 14.092 mg/L, and 57.362 min, respectively. Considering the promising photocatalytic activity of FZT Y@WDS under visible light and the separation performance of the nanocomposite, we proposed this photocatalyst as an alternative solution for the treatment of pharmaceutical wastewater.
Schistosoma japonicum-derived peptide SJMHE1 ameliorates allergic symptoms and responses in mice with allergic rhinitis
Authors: Gao Xuerong , Mao Chaoming , Zheng Tingting , Xu Xiaowei , Luo Xinkai , Zhang Shan , Liu Jiameng , Wang Xuefeng , Chen Xiaojun , Dong Liyang
Published: June 2023

Helminth derived excretory/secretory molecules have shown efficacy in the treatment of allergic asthma in mice, but their roles in allergic rhinitis (AR) are little known. In this study, we aimed to determine the intervention effect of SJMHE1, a Schistosoma japonicum derived small molecular peptide, on ovalbumin (OVA)-induced AR mice and investigate its possible mechanism. AR was induced in BALB/c mice, following which the mice were treated with phosphate-buffered saline (PBS), OVA323-339 and SJMHE1 respectively. SJMHE1 treatment improved clinical symptoms (rubbing and sneezing), suppressed infiltrates of inflammatory cells and eosinophils in nasal mucosa, modulated the production of type-2 (IL-4 and IL-13) and anti-inflammatory (IL-10) cytokines in the nasal lavage fluids (NLF), spleen, and serum. To investigate the underlying mechanism, fluorescein isothiocyanate (FITC)-labeled SJMHE1 was subcutaneously injected into AR mice, and we found that the FITC-SJMHE1 could accumulate in spleen, but not in nasal mucosa. FITC-SJMHE1 mainly bound to CD19 positive cells (B cells), and the SJMHE1 treatment significantly increased the proportion of regulatory B cells (Bregs) and B10 cells, along with the enhancement of PR domain containing protein 1 (Prdm1) protein levels. SJMHE1 may alleviate AR by upregulating Bregs, and has great potential as a new avenue for the AR treatment.
For the girls, gays, and theys: LGBTQ+ stakeholder communication and alignment of video game brands
Authors: Gaudszun Isabell Charlott , Elmezeny Ahmed
Published: June 2023

Queer identities have predominantly existed at the peripheries of media representation as well as communication research. Linking this research gap with the field of video games as a medium, this study examines video game companies and their strategic communication efforts toward LGBTQ+ stakeholders through critical discourse analysis with influences of queer theory. With this focus, we aim to identify how video game companies discursively construct queer identities and utilize them for strategic communication. Through the analysis of the social media and online discourses surrounding two games with queer inclusion, we show that queer branding has an influence on the way strategic communication efforts are employed throughout the year, as well as the type of content. Furthermore, we show that in our selected cases, organizations can successfully align themselves with their LGBTQ+ stakeholders by having queer inclusion at the core of their strategic communication efforts and authentic organizational practices.
Incidence and predictors of hemodynamic compromise due to high-grade AV block after TAVI
Authors: Weferling Maren , Lan Cheong Wah Stefan , Fischer-Rasokat Ulrich , Hain Andreas , Renker Matthias , Charitos Efstratios I. , Liebetrau Christoph , Treiber Julia , Choi Yeong-Hoon , Hamm Christian W. , Kim Won-Keun
Published: June 2023

Background

High-grade AV block (HAVB) is the most frequent adverse event after transcatheter aortic valve implantation (TAVI). In rare cases, HAVB is associated with hemodynamic compromise (HC) followed by syncope or application of cardiopulmonary resuscitation (CPR), but data on this severe complication are scarce. The aim of the present study was to investigate the incidence and predictors of HC due to HAVB in patients undergoing TAVI.

Methods

In this retrospective analysis of 4,602 TAVI cases between 2010 and 2022, 466 developed HAVB. Baseline characteristics and procedural and postprocedural findings were compared for patients with HC versus those without. Univariate and multivariable regression analyses were used to investigate independent predictors of HC.

Results

Forty-nine of 466 patients (10.5%) had HC due to HAVB after TAVI. Patients with HC had a longer hospital stay [10 (8–13) vs. 13 (9–18) days; p < 0.001], more frequent peripheral artery disease (PAD) (28.6% vs. 15.1%; p = 0.016), and lower hemoglobin levels [11.8 (±) vs. 12.5 (±) g/dl; p = 0.006]. In the HC group, HAVB onset post-TAVI was delayed compared with the non-HC group [2 (1–4) vs. 1 (0–3) days; p < 0.001]. Before HAVB onset, patients in the HC group more frequently developed post-TAVI delirium [18 (4.6%) vs. 11 (25.0%); p < 0.001]. In univariate regression analysis, PAD, hemoglobin, procedural time, contrast agent volume, and post-TAVI delirium were significant predictors of HC. After adjustment, only post-TAVI delirium and contrast agent volume remained independent predictors [OR 3.22 (95% CI: 1.05–9.89); p = 0.042 and OR: 1.01 (95% CI: 1.0–1.01); p = 0.04, respectively].

Conclusion

HC due to HAVB after TAVI occurred in over 10% of cases. Development of post-TAVI delirium and contrast agent volume are independent predictors of this severe complication.