bluuebunny/crossref_metadata_2025 huggingface dataset:
Modeling the Geometry of Tree Trunks Using LiDAR Data
Authors: Tarsha Kurdi Fayez , Gharineiat Zahra , Lewandowicz Elżbieta , Shan Jie
Published: February 2024

The effective development of digital twins of real-world objects requires sophisticated data collection techniques and algorithms for the automated modeling of individual objects. In City Information Modeling (CIM) systems, individual buildings can be modeled automatically at the second Level of Detail or LOD2. Similarly, for Tree Information Modeling (TIM) and building Forest Digital Twins (FDT), automated solutions for the 3D modeling of individual trees at different levels of detail are required. The existing algorithms support the automated modeling of trees by generating models of the canopy and the lower part of the trunk. Our argument for this work is that the structure of tree trunk and branches is as important as canopy shape. As such, the aim of the research is to develop an algorithm for automatically modeling tree trunks based on data from point clouds obtained through laser scanning. Aiming to generate 3D models of tree trunks, the suggested approach starts with extracting the trunk point cloud, which is then segmented into single stems. Subsets of point clouds, representing individual branches, are measured using Airborne Laser Scanning (ALS) and Terrestrial Laser Scanning (TLS). Trunks and branches are generated by fitting cylinders to the layered subsets of the point cloud. The individual stems are modeled by a structure of slices. The accuracy of the model is calculated by determining the fitness of cylinders to the point cloud. Despite the huge variation in trunk geometric forms, the proposed modeling approach can gain an accuracy of better than 4 cm in the constructed tree trunk models. As the developed tree models are represented in a matrix format, the solution enables automatic comparisons of tree elements over time, which is necessary for monitoring changes in forest stands. Due to the existence of large variations in tree trunk geometry, the performance of the proposed modeling approach deserves further investigation on its generality to other types of trees in multiple areas.
Forest Canopy Height Estimation by Integrating Structural Equation Modeling and Multiple Weighted Regression
Authors: Zhu Hongbo , Zhang Bing , Song Weidong , Xie Qinghua , Chang Xinyue , Zhao Ruishan
Published: February 2024

As an important component of forest parameters, forest canopy height is of great significance to the study of forest carbon stocks and carbon cycle status. There is an increasing interest in obtaining large-scale forest canopy height quickly and accurately. Therefore, many studies have aimed to address this issue by proposing machine learning models that accurately invert forest canopy height. However, most of the these approaches feature PolSAR observations from a data-driven viewpoint in the feature selection part of the machine learning model, without taking into account the intrinsic mechanisms of PolSAR polarization observation variables. In this work, we evaluated the correlations between eight polarization observation variables, namely, T11, T22, T33, total backscattered power (SPAN), radar vegetation index (RVI), the surface scattering component (Ps), dihedral angle scattering component (Pd), and body scattering component (Pv) of Freeman-Durden three-component decomposition, and the height of the forest canopy. On this basis, a weighted inversion method for determining forest canopy height under the view of structural equation modeling was proposed. In this study, the direct and indirect contributions of the above eight polarization observation variables to the forest canopy height inversion task were estimated based on structural equation modeling. Among them, the indirect contributions were generated by the interactions between the variables and ultimately had an impact on the forest canopy height inversion. In this study, the covariance matrix between polarization variables and forest canopy height was calculated based on structural equation modeling, the weights of the variables were calculated by combining with the Mahalanobis distance, and the weighted inversion of forest canopy height was carried out using PSO-SVR. In this study, some experiments were carried out using three Gaofen-3 satellite (GF-3) images and ICESat-2 forest canopy height data for some forest areas of Gaofeng Ridge, Baisha Lizu Autonomous County, Hainan Province, China. The results showed that T11, T33, and total backscattered power (SPAN) are highly correlated with forest canopy height. In addition, this study showed that determining the weights of different polarization observation variables contributes positively to the accurate estimation of forest canopy height. The forest canopy height-weighted inversion method proposed in this paper was shown to be superior to the multiple regression model, with a 26% improvement in r and a 0.88 m reduction in the root-mean-square error (RMSE).
Spatio-Temporal Dynamics of Normalized Difference Vegetation Index and Its Response to Climate Change in Xinjiang, 2000–2022
Authors: Zhang Qianqian , Gu Lei , Liu Yongqiang , Zhang Yongfu
Published: February 2024

Based on the NDVI and climate data from 2000 to 2022, this study systematically investigated the spatial and temporal patterns, trend characteristics, and stability of the NDVI in Xinjiang using the one-way linear regression method, Theil–Sen Median trend analysis, the Mann–Kendall significance test, and the coefficient of variation. Meanwhile, the persistence of the NDVI distribution was analyzed by combining the trend results and Hurst index. Finally, partial correlation analysis was used to deeply explore the response mechanisms of interannual and seasonal-scale NDVI and climatic factors in Xinjiang, and the characteristics of multi-year vegetation distribution were comprehensively analyzed with the help of human footprint data. The findings indicate the following: (1) The NDVI of interannual and seasonal vegetation in Xinjiang showed a significant increasing trend during the 23-year period, but the spatial distribution was heterogeneous, and the improvement of the vegetation condition in the southern part of the region was remarkable. (2) The NDVI is relatively stable across the region. Unlike in other regions, in general, it is difficult to maintain the existing trend in NDVI in the study area for a long period of time, and the reverse trend is more persistent. (3) On the interannual scale, both precipitation and temperature are positively correlated with the NDVI, and the influence of temperature (80.94%) is greater than that of precipitation (63.82%). Precipitation was dominantly positively correlated with the NDVI in spring, summer, and the growing season, while it was negatively correlated with it in autumn. Temperature and NDVI were positively correlated, with the greatest influence in the spring. (4) Human activities had the greatest impact on the areas with low vegetation cover and areas with medium–low vegetation cover, and there was a high degree of overlap between the areas where the interannual human footprints and NDVI showed an increasing trend. The percentage of human footprints that significantly correlated with interannual NDVI was 34.79%. In the future, the protection and management of ecologically fragile areas should be increased to increase desert-vegetation cover.
Mechanical and Antibacterial Properties of Bamboo Charcoal/ZnO-Modified Bamboo Fiber/Polylactic Acid Composites
Authors: Liu Chunlin , Zhang Shuai , Yan Shi , Pan Mingzhu , Huang Hui
Published: February 2024

In this study, biodegradable bamboo fiber/PLA composites (BPCs) modified using bamboo charcoal (BC)/ZnO were prepared. The effects of BC/ZnO addition on the mechanical properties and antibacterial properties of BPCs were investigated. The chemical structure, microscopic morphology, and crystallization of the composites were analyzed using FTIR, SEM, and XRD, respectively. The results showed that in terms of mechanical strength, when the addition of BC was 2%, the tensile impact and flexural strength of the BPCs were most obviously improved, with a tensile strength of 51.6 MPa. However, when the addition of BC was more than 2%, the uneven dispersion of too much BC in the BPCs resulted in a reduction in their mechanical strength. A certain amount of ZnO did not affect the crystallinity of the BPCs. In addition, the uneven distribution of ZnO and its poor compatibility with PLA resulted in a deterioration in the tensile properties of the BPCs. In terms of antibacterial properties, when 2% ZnO was added, the BPCs had better antibacterial properties against Escherichia coli and Staphylococcus aureus, with values of 58.9% and 52.5% against both, respectively. BPC biodegradable materials with both mechanical strength and antimicrobial properties have promising medical uses.
Enhancing Banana Flour Quality through Physical Modifications and Its Application in Gluten-Free Chips Product
Authors: Kunyanee Kannika , Van Ngo Tai , Kusumawardani Sandra , Luangsakul Naphatrapi
Published: February 2024

The objective of this study was to analyze the effects of different single or dual physical treatments, including pre-gelatinization (PBF), annealing (ANN), PBF+ANN, and ANN+PBF, on banana flour’s characteristics and its application in gluten-free chip production. The study involved determining the color, swelling capacity, solubility, oil absorption index, and pasting properties of both the native and modified banana flour samples. The results showed a significant change in color, particularly in the pre-gelatinized samples. There was a noticeable decrease in the values of the pasting parameters in the modified samples. PBF samples exhibited a remarkable reduction in the breakdown value compared to the native and ANN treated samples. Furthermore, PBF-treated banana flour displayed higher oil absorption and swelling power than the other samples, along with lower solubility in the PBF-treated sample. These characteristics appear to be responsible for enabling the pre-gelatinized sample to form the dough required for producing banana chips, resulting in distinct texture profiles. Finally, our research emphasizes the useful application of modified banana flour in the food industry and emphasizes how crucial it is to choose the right modification method to achieve the desired effects on the product.
Improving Tenebrio molitor Growth and Nutritional Value through Vegetable Waste Supplementation
Authors: López-Gámez Gloria , del Pino-García Raquel , López-Bascón María Asunción , Verardo Vito
Published: February 2024

Huge amounts of vegetable wastes are generated by the food industry. Their bioconversion into valuable products (e.g., insect flours or biofertilizer) through insect farming is a promising solution to reduce their negative environmental and economic impacts. This study evaluates the growth of Tenebrio molitor larvae and their nutritional profile after supplementing their diets with vegetable wastes. Over a 6-week period, 45-day larvae were fed a diet comprising wheat bran supplemented (1:1) with cucumber or tomato wastes from both conventional and ecological crops. The control diet consisted of wheat bran and an equivalent amount of water to compensate for the waste moisture. Larval weight was measured weekly, and length measures were taken fortnightly. Nutritional composition and fatty acid profile were analyzed at the end of the study in 90-day larvae. Regardless of using vegetable waste from conventional or ecological harvesting, the weight of 6-week supplemented larvae almost doubled that of larvae fed with just wheat bran, and their length was 15% higher. Supplementation also increased larval polyunsaturated fatty acid percentage by 22–37%, with linoleic acid being the most abundant. Likewise, larval protein content reached 50% after supplementation. This study demonstrates that both cucumber and tomato wastes from conventional or ecological crops are excellent supplements for T. molitor’s diet, improving their nutritional value and reducing the time necessary for larvae growth.
Ultrasensitive Ochratoxin A Detection in Cereal Products Using a Fluorescent Aptasensor Based on RecJf Exonuclease-Assisted Target Recycling
Authors: Li Yanxuan , Shao Furong , Wu Jin , Liu Mingzhu , Cao Gaofang , Zhao Zunquan , Bai Jialei , Gao Zhixian
Published: February 2024

Ochratoxin A (OTA) is a mycotoxin widely found in foodstuffs such as cereal grains. It greatly threatens human health owing to its strong toxicity and high stability. Aptasensors have emerged as promising tools for the analysis of small molecule contaminants. Nucleic-acid-based signal amplification enables detectable signals to be obtained from aptasensors. However, this strategy often requires the use of complex primers or multiple enzymes, entailing problems such as complex system instability. Herein, we propose a fluorescent aptasensor for the ultrasensitive detection of OTA in cereal products, with signal amplification through RecJf exonuclease-assisted target recycling. The aptamer/fluorescein-labeled complementary DNA (cDNA-FAM) duplex was effectively used as the target-recognition unit as well as the potential substrate for RecJf exonuclease cleavage. When the target invaded the aptamer-cDNA-FAM duplex to release cDNA-FAM, RecJf exonuclease could cleave the aptamer bonded with the target and release the target. Thus, the target-triggered cleavage cycling would continuously generate cDNA-FAM as a signaling group, specifically amplifying the response signal. The proposed exonuclease-assisted fluorescent aptasensor exhibited a good linear relationship with OTA concentration in the range from 1 pg/mL to 10 ng/mL with an ultralow limit of detection (6.2 ng/kg of cereal). The analytical method showed that recoveries of the cereal samples ranged from 83.7 to 109.3% with a repeatability relative standard deviation below 8%. Importantly, the proposed strategy is expected to become a common detection model because it can be adapted for other targets by replacing the aptamer. Thus, this model can guide the development of facile approaches for point-of-care testing applications.
Functional and Antioxidative Characteristics of Soft Wheat and Tiger Nut (Cyperus esculentus) Flours Binary Blends
Authors: Nedviha Svitlana , Harasym Joanna
Published: February 2024

Tiger nut (Cyperus esculentus) or chufa is little known plant of high nutritious content. Popularized by a plant-based drink called “horchata de chufa,” it still offers a lot to research, being abundant in fat, starch, fiber and minerals and vitamins. To properly adjust this raw material to new purposes, the knowledge of crucial properties of the most basic blends like with soft wheat flour is needed. This article evaluates the techno-functional, viscometrical and bioactive characteristics of soft wheat:tiger nut blends of 5%, 10%, 15%, 20% and 25%. Granulometry, water-holding capacity (WHC), water absorption capacity (WAC), water absorption index (WAI), water solubility index (WSI), oil absorption capacity (OAC), hydrophilic/lipophilic index (HLI), color, pasting properties, total polyphenol content (TPC), antioxidant activity (DPPH), reducing sugars content and dough-rising capacity were assessed. The addition of tiger nut improved total polyphenol content of blends, however, It was observed that the addition of tiger nuts raised the total polyphenol content of the mixtures, but this was not statistically significant despite as much as 25% of tiger nuts. Oppositely, antioxidant activity was gradually improved with increasing tiger nut content. Pasting properties were impacted by tiger nut addition, lowering both pasting viscosity and trough viscosity, however, final viscosity was not particularly affected, being lowered by less than 15%. The highest water absorption was noted for 100% tiger nut both for WHC and WAC, however, WAI was the lowest for this sample. All the blends with tiger nut revealed improved dough-rising profile.
The Potential of Crude and Partially Purified Black Rice Bran Extracts Obtained by Ultrasound-Assisted Extraction: Anti-Glycemic, Cytotoxicity, Cytoprotective, and Antitumoral Effects
Authors: Leonarski Eduardo , Kuasnei Mayara , Santos Eloisa H. , Moraes Paulo A. D. , Cesca Karina , Oliveira Débora de , Zielinski Acácio A. F.
Published: February 2024

Recovering anthocyanins from black rice bran is a way of valuing this byproduct, by obtaining an extract with biological potential. The objective of this study was to recover anthocyanins using ultrasound-assisted extraction. Some of the extract was partially purified, and both (crude and partially purified) extracts were evaluated for their anthocyanin content, antioxidant activity, antidiabetic and antitumoral activities, cytotoxicity, and oxidative stress. An increase in the laboratory scale was also achieved, making possible to increase the extraction volume up to 20 times without significantly changing the content of anthocyanins (1.85 mg C3G/g DW). It was found that the purified sample presented a 4.2 times higher value of total anthocyanins compared to the crude sample. The best IC50 values for the purified sample were verified by DPPH and ABTS (0.76 and 0.33 mg/mL). The best results for antidiabetic activity were obtained for the partially purified sample: 0.82 µM C3G for α-glucosidase and 12.5 µM C3G for α-amylase. The extracts demonstrated protection (~70%) when subjected to the oxidative stress of L929 cells. An antitumoral effect of 25–30% for both extracts was found in A459 cells. The crude and partially purified extracts of black rice have antidiabetic and anticancer effects and more studies are needed to explore their potential.
Effects of Litsea cubeba Essential Oil–Chitosan/Corn Starch Composite Films on the Quality and Shelf-Life of Strawberry (Fragaria × ananassa)
Authors: Fu Hongjun , Wang Liyuan , Gu Jiahui , Peng Xianglian , Zhao Jian
Published: February 2024

In this work, we have developed a composite chitosan film incorporating the Litsea cubeba essential oil (LCEO) and starch with good physical properties, and investigated the effect of coating strawberries with this composite film. The best formula of the LCEO/chitosan/corn starch/glycerol (LCEO/CH/CS/gly) composite films is 0.25% LCEO, 2.75% CH, 0.40% corn starch, and 0.75% glycerol. Coating strawberries with CH/CS/gly film or LCEO/CH/CS/gly films resulted in significantly lower respiration intensity and a slower decay rate, much slower decreases in the firmness, and reductions in the sugar and ascorbic acid content of the fruit during storage (p < 0.05). The coatings also led to a much slower accumulation of malondialdehyde and anthocyanins (p < 0.05). The LCEO/CH/CS/gly film was generally more effective than the CH/CS/gly film; however, the effect was more obvious in the later stages of storage. Thus, coating strawberries with CH/CS/gly film or LCEO/CH/CS/gly film can be a viable method for extending the shelf-life of the fruit.
Electrostatic Fermentation: Molecular Response Insights for Tailored Beer Production
Authors: Amaro-Reyes Aldo , Marcial-Ramírez Diana , Vázquez-Landaverde Pedro Alberto , Utrilla José , Escamilla-García Monserrat , Regalado Carlos , Macias-Bobadilla Gonzalo , Campos-Guillén Juan , Ramos-López Miguel Angel , Favela-Camacho Sarai E.
Published: February 2024

Electrostatic fermentation avoids the cellular redox imbalance of traditional fermentation, but knowledge gaps exist. This study explores the impact of electrostatic fermentation on the growth, volatile profile, and genetic response of Saccharomyces pastorianus Saflager S-23. The applied voltage (15 and 30 V) in the electrostatic fermentation system increased the growth and substrate utilization of S. pastorianus while decreasing ethanol production. The aromas typically associated with traditional fermentation, such as alcoholic, grape, apple, and sweet notes, were diminished, while aromas like roses, fruits, flowers, and bananas were augmented in electrostatic fermentation. RNA-seq analysis revealed upregulation of genes involved in cell wall structure, oxidoreductase activity, and iron ion binding, while genes associated with protein synthesis, growth control, homeostasis, and membrane function were downregulated under the influence of applied voltage. The electrostatic fermentation system modulates genetic responses and metabolic pathways in yeast, rendering it a promising method for tailored beer production. Demonstrating feasibility under industrial-scale and realistic conditions is crucial for advancing towards commercialization.
Evolution Pattern in Bruised Tissue of ‘Red Delicious’ Apple
Authors: Xu Tao , Zhang Xiaomin , Zhu Yihang , Xu Xufeng , Rao Xiuqin
Published: February 2024

The study of apple damage mechanisms is key to improving post-harvest apple treatment techniques, and the evolution pattern of damaged tissue is fundamental to the study of apple damage mechanisms. In the study, ‘Red Delicious’ apples were used to explore the relationship between damage and time. A cell death zone was found in the pulp of the damaged tissue after the apple had been bruised. The tissue damage was centered in the cell death zone and developed laterally, with the width of the damage increasing with injury time. The extent of tissue damage in the core and pericarpal directions varied. About 60% of the damaged tissue developed in the core direction and 40% in the pericarpal direction, and the damage ratios in both directions remained consistent throughout the injury. The depth of damage and the rate of damage were influenced by the impact force size and the difference in the size of the damaged part of the apple, but the damage development pattern was independent of the impact force size and the difference in the damaged part. The maximum damage rate was reached at about 30 min, and the depth of damage was stabilized at about 72 min. By studying the evolution pattern of the damaged tissue of the bruised ‘Red Delicious’ apple, it provides the research idea and theoretical basis for enhancing the prediction accuracy and robustness of early stage damage in apples.
Olive Pomace Oil Structuring for the Development of Healthy Puff Pastry Laminating Fats: The Effect of Chilling Storage on the Quality of Baked Products
Authors: Álvarez María Dolores , Saiz Arancha , Herranz Beatriz , Cofrades Susana
Published: February 2024

Developing puff pastry (PP) laminating fats (LFs) with sustainable structured olive pomace oil (OPO) could contribute to its increased valorization. This study evaluated the physicochemical stability of four OPO-based LFs or margarines and the performance of their baked PP counterparts during two months of chilling storage at 4 °C. LF samples, developed at the laboratory scale, contained 41% (LF1 and LF2) OPO and 31% (LF3 and LF4) OPO together with 10% cocoa butter when using two static initial crystallization conditions (room temperature for LF1 and LF3, freezer for LF2 and LF4) before storage. During the storage period, the proximate composition, thermal and dynamic rheological properties, firmness and spreadability, oil-binding capacity, color, and lipid oxidation of the four LF samples were examined, along with the baking performance and textural properties of the PP counterparts. The initial cooling rate had minimal significance. Cocoa butter negatively influenced post-crystallization processes occurring in OPO-based LF3 and LF4, resulting in increased hardness and reduced performance after 18 days of storage, attributed, at least partially, to a high amount of 1,3-dipalmitoyl-2-oleoyl-glycerol (POP), mainly from cocoa butter. Conversely, OPO-based LF1 and LF2 maintained their quality and were stable for two months without apparent granular crystal formation.
Hosts, Again: From Conditional Inclusion and Liberal Censorship to Togetherness and Creative/Critical Refugee Epistemologies
Authors: Hodžić Saida
Published: February 2024

In this experimental text that critically juxtaposes autoethnographic narration, reflection, and analysis with theoretical engagements, I suggest that the power dynamics that diminish and dispossess the lives of refugees and other displaced people also constrain and censor critical refugee epistemologies. Refugees are frequently impelled to speak, implored to speak, coached to speak, interrogated and ordered to speak, but on the condition that we consent to having our voices policed. Our narratives are welcomed if they affirm the humanitarian liberal order, but the knowledge we possess challenges it. Presented as benevolent and caring, the incessant demands for refugee stories and trauma erotics are also mechanisms of putting refugees in place: they assign the refugee a subject position of a conditionally accepted narrator who is refused authorship and self-possession. Our narratives fail to count as knowledge unless they are converted into writing by citizen ghost writers or coauthors. And when we refuse to recite trauma stories and instead disrupt the order of things by critically analyzing violent regimes of refuge and liberal complicity, we are censored. Refugees have things to say as ethnographers of their own lives, analysts of upside-down mobility, and critics of violent bureaucracies. This knowledge is needed and wanted. Rather than orienting our work to liberal publics, we are creating alternative, self-authorized structures that uphold displaced people as knowledgeable and world-building subjects, as people able to host others.
An Environmental Equity Assessment Using a Social Vulnerability Index during the SARS-CoV-2 Pandemic for Siting of Wastewater-Based Epidemiology Locations in the United States
Authors: Mosher Jessica R. , Banta Jim E. , Spencer-Hwang Rhonda , Naughton Colleen C. , Kadonsky Krystin F. , Hile Thomas , Sinclair Ryan G.
Published: February 2024

Research has shown that there has consistently been a lack of equity and accessibility to SARS-CoV-2 testing in underserved and disadvantaged areas in the United States. This study examines the distribution of Wastewater-Based Epidemiology (WBE) testing placement across the United States (US), particularly within the context of underserved communities, and explores an environmental equity approach to address the impact of WBE on future pandemics. The methods combined the Centers for Disease Control Social Vulnerability Index (CDC-SVI) data set at the county level in a geospatial analysis utilizing ArcGIS and multilinear regression analysis as independent variables to investigate disparities in WBE coverage in the US. The findings show that disparities exist between counties in the use of WBE nationwide. The results show that WBE is distributed inequitably on national and state levels. Considering the nationwide adoption of WBE and funding availability through the CDC National Wastewater Surveillance System, these findings underscore the importance of equitable WBE coverage for effective COVID-19 monitoring. These findings offer data to support that a focus on expanding WBE coverage to underserved communities ensures a proactive and inclusive strategy against future pandemics.
Ground Truth in Classification Accuracy Assessment: Myth and Reality
Authors: Foody Giles M.
Published: February 2024

The ground reference dataset used in the assessment of classification accuracy is typically assumed implicitly to be perfect (i.e., 100% correct and representing ground truth). Rarely is this assumption valid, and errors in the ground dataset can cause the apparent accuracy of a classification to differ greatly from reality. The effect of variations in the quality in the ground dataset and of class abundance on accuracy assessment is explored. Using simulations of realistic scenarios encountered in remote sensing, it is shown that substantial bias can be introduced into a study through the use of an imperfect ground dataset. Specifically, estimates of accuracy on a per-class and overall basis, as well as of a derived variable, class areal extent, can be biased as a result of ground data error. The specific impacts of ground data error vary with the magnitude and nature of the errors, as well as the relative abundance of the classes. The community is urged to be wary of direct interpretation of accuracy assessments and to seek to address the problems that arise from the use of imperfect ground data.
Reflections about Blockchain in Health Data Sharing: Navigating a Disruptive Technology
Authors: Corte-Real Ana , Nunes Tiago , da Cunha Paulo Rupino
Published: February 2024

A comprehensive analysis was performed, considering blockchain technology (BT) properties in digital health, addressing medicolegal, privacy, and regulatory considerations. Adherence to personal data protection and healthcare regulatory guidelines were analyzed and compared for GDPR (Europe), HIPAA (United States), CCPA (California), PIPEDA (Canada), the Privacy Act of 1988 (Australia), APPI (Japan), and LGPD (Brazil). Issues such as health systems, strengthening and aligning policy orientations and initiatives, and emphasizing the role of data analysis in shaping health policies were explored. The study addressed conflicts between the legal frameworks and blockchain, comparing and suggesting solutions like the revision of laws and the integration of compliance mechanisms. Additionally, it sought to enhance IT-health literacy by integrating the healthcare and legal domains. Ongoing collaboration between legal, health, and IT experts is essential for designing systems that effectively balance privacy rights and data protection while maximizing the benefits of disruptive technologies like blockchain.
U.S. Physicians’ Training and Experience in Providing Trauma-Informed Care in Clinical Settings
Authors: Novilla M. Lelinneth B. , Bird Kaitlyn Tan , Hanson Carl L. , Crandall AliceAnn , Cook Ella Gaskin , Obalana Oluwadamilola , Brady Lexi Athena , Frierichs Hunter
Published: February 2024

Trauma-informed care (TIC) is a comprehensive approach that focuses on the whole individual. It acknowledges the experiences and symptoms of trauma and their impact on health. TIC prioritizes physical and emotional safety through a relationship of trust that supports patient choice and empowerment. It provides a safe and respectful healing environment that considers specific needs while promoting a greater sense of well-being, patient engagement, and partnership in the treatment process. Given the prevalence of trauma, this descriptive cross-sectional study examined the attitudes and perspectives of U.S. physicians (N = 179; 67% males; 84% White; 43% aged 56–65) in providing trauma-informed care using an anonymous 29-item online survey administered by Reaction Data. Findings showed that 16% (n = 18) of physicians estimated that >50% of their patients have a history of trauma. Commonly perceived barriers to providing TIC were resource/time/administrative constraints, provider stress, limited awareness of the right provider to refer patients who experienced trauma, and inadequate TIC emphasis in medical education/training. Expanding physicians’ knowledge base of trauma through training and organizational policy/support is crucial in enhancing their TIC competence, particularly in caring for patients with complex care needs whose social determinants increase their risk of exposure to adverse experiences that carry lasting physical and psychological effects.
Respiratory Dysfunction in Alzheimer’s Disease—Consequence or Underlying Cause? Applying Animal Models to the Study of Respiratory Malfunctions
Authors: Wrzesień Agnieszka , Andrzejewski Kryspin , Jampolska Monika , Kaczyńska Katarzyna
Published: February 2024

Alzheimer’s disease (AD) is a neurodegenerative brain disease that is the most common cause of dementia among the elderly. In addition to dementia, which is the loss of cognitive function, including thinking, remembering, and reasoning, and behavioral abilities, AD patients also experience respiratory disturbances. The most common respiratory problems observed in AD patients are pneumonia, shortness of breath, respiratory muscle weakness, and obstructive sleep apnea (OSA). The latter is considered an outcome of Alzheimer’s disease and is suggested to be a causative factor. While this narrative review addresses the bidirectional relationship between obstructive sleep apnea and Alzheimer’s disease and reports on existing studies describing the most common respiratory disorders found in patients with Alzheimer’s disease, its main purpose is to review all currently available studies using animal models of Alzheimer’s disease to study respiratory impairments. These studies on animal models of AD are few in number but are crucial for establishing mechanisms, causation, implementing potential therapies for respiratory disorders, and ultimately applying these findings to clinical practice. This review summarizes what is already known in the context of research on respiratory disorders in animal models, while pointing out directions for future research.
Novel Enhanced Mammalian Cell Transient Expression Vector via Promoter Combination
Authors: Yoon SunKyung , Park SeJin , Lee JuneWoo , Kim Byoungguk , Gwak WonSeok
Published: February 2024

During the emergence of infectious diseases, evaluating the efficacy of newly developed vaccines requires antigen proteins. Available methods enhance antigen protein productivity; however, structural modifications may occur. Therefore, we aimed to construct a novel transient overexpression vector capable of rapidly producing large quantities of antigenic proteins in mammalian cell lines. This involved expanding beyond the exclusive use of the human cytomegalovirus (CMV) promoter, and was achieved by incorporating a transcriptional enhancer (CMV enhancer), a translational enhancer (woodchuck hepatitis virus post-transcriptional regulatory element), and a promoter based on the CMV promoter. Twenty novel transient expression vectors were constructed, with the vector containing the human elongation factor 1-alpha (EF-1a) promoter showing the highest efficiency in expressing foreign proteins. This vector exhibited an approximately 27-fold higher expression of enhanced green fluorescent protein than the control vector containing only the CMV promoter. It also expressed the highest level of severe acute respiratory syndrome coronavirus 2 receptor-binding domain protein. These observations possibly result from the simultaneous enhancement of the transcriptional activity of the CMV promoter and the human EF-1a promoter by the CMV enhancer. Additionally, the synergistic effect between the CMV and human EF-1a promoters likely contributed to the further enhancement of protein expression.
Pathophysiology of Diet-Induced Acid Stress
Authors: Goraya Nimrit , Wesson Donald E.
Published: February 2024

Diets can influence the body’s acid–base status because specific food components yield acids, bases, or neither when metabolized. Animal-sourced foods yield acids and plant-sourced food, particularly fruits and vegetables, generally yield bases when metabolized. Modern diets proportionately contain more animal-sourced than plant-sourced foods, are, thereby, generally net acid-producing, and so constitute an ongoing acid challenge. Acid accumulation severe enough to reduce serum bicarbonate concentration, i.e., manifesting as chronic metabolic acidosis, the most extreme end of the continuum of “acid stress”, harms bones and muscles and appears to enhance the progression of chronic kidney disease (CKD). Progressive acid accumulation that does not achieve the threshold amount necessary to cause chronic metabolic acidosis also appears to have deleterious effects. Specifically, identifiable acid retention without reduced serum bicarbonate concentration, which, in this review, we will call “covert acidosis”, appears to cause kidney injury and exacerbate CKD progression. Furthermore, the chronic engagement of mechanisms to mitigate the ongoing acid challenge of modern diets also appears to threaten health, including kidney health. This review describes the full continuum of “acid stress” to which modern diets contribute and the mechanisms by which acid stress challenges health. Ongoing research will develop clinically useful tools to identify stages of acid stress earlier than metabolic acidosis and determine if dietary acid reduction lowers or eliminates the threats to health that these diets appear to cause.
Anserine, a Histidine-Containing Dipeptide, Suppresses Pressure Overload-Induced Systolic Dysfunction by Inhibiting Histone Acetyltransferase Activity of p300 in Mice
Authors: Sunagawa Yoichi , Tsukabe Ryosuke , Irokawa Yudai , Funamoto Masafumi , Suzuki Yuto , Yamada Miho , Shimizu Satoshi , Katanasaka Yasufumi , Hamabe-Horiike Toshihide , Kawase Yuto , Naruta Ryuya , Shimizu Kana , Mori Kiyoshi , Hosomi Ryota , Komiyama Maki , Hasegawa Koji , Morimoto Tatsuya
Published: February 2024

Anserine, an imidazole dipeptide, is present in the muscles of birds and fish and has various bioactivities, such as anti-inflammatory and anti-fatigue effects. However, the effect of anserine on the development of heart failure remains unknown. We cultured primary cardiomyocytes with 0.03 mM to 10 mM anserine and stimulated them with phenylephrine for 48 h. Anserine significantly suppressed the phenylephrine-induced increases in cardiomyocyte hypertrophy, ANF and BNP mRNA levels, and histone H3K9 acetylation. An in vitro histone acetyltransferase (HAT) assay showed that anserine directly suppressed p300-HAT activity with an IC50 of 1.87 mM. Subsequently, 8-week-old male C57BL/6J mice were subjected to transverse aortic constriction (TAC) and were randomly assigned to receive daily oral treatment with anserine-containing material, Marine Active® (60 or 200 mg/kg anserine) or vehicle for 8 weeks. Echocardiography revealed that anserine 200 mg/kg significantly prevented the TAC-induced increase in left ventricular posterior wall thickness and the decrease in left ventricular fractional shortening. Moreover, anserine significantly suppressed the TAC-induced acetylation of histone H3K9. These results indicate that anserine suppresses TAC-induced systolic dysfunction, at least in part, by inhibiting p300-HAT activity. Anserine may be used as a pharmacological agent for human heart failure therapy.
Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells
Authors: Iribarne Ailén , Palma María Belén , Andrini Laura , Riccillo Fernando , Rodriguez Delfina , Casella Martín , Garay Felipe , Zabala Julieta Spoto , Mazza Leandro , Muro Adriana , Buero Guillermo , Miriuka Santiago G. , Carosella Edgardo , García Marcela N.
Published: February 2024

Wound healing after skin injury is a complex process, particularly in equines where leg wounds are prevalent and their repair is complicated due to the anatomical characteristics. Conventional treatments are not effective enough. The umbilical cord offers an unlimited source of adult mesenchymal stem cells (ucMSCs) from Wharton’s jelly tissue. The present study aims to demonstrate the safety and therapeutic potential of the allogeneic use of equine ucMSCs (e-ucMSCs) in the healing of severe equine leg wounds. The methods employed were the isolation, culture and expansion of e-ucMSCs. Flow cytometry and a PCR assay were used for cell characterization. This study included an immunomodulation assay, a murine pre-clinical trial and the first phase of an equine clinical trial. Our results showed that e-ucMSCs express a functional HLA-G homolog, EQMHCB2. In the immunomodulation assay, the e-ucMSCs inhibited the proliferation of activated equine peripheral blood mononuclear cells (e-PBMCs). In the murine pre-clinical trial, e-ucMSCs reduced healing time by 50%. In the equine clinical trial, the injection of e-ucMSCs into severe leg lesions improved the closure time and quality of the tissues involved, regenerating them without fibrous tissue scar formation. In conclusion, the results of this study suggest that e-ucMSCs can be used allogeneically for wound healing by creating a tolerogenic environment.
Leveraging Artificial Intelligence and Participatory Modeling to Support Paradigm Shifts in Public Health: An Application to Obesity and Evidence-Based Policymaking
Authors: Giabbanelli Philippe J. , MacEwan Grace
Published: February 2024

The Provincial Health Services Authority (PHSA) of British Columbia suggested that a paradigm shift from weight to well-being could address the unintended consequences of focusing on obesity and improve the outcomes of efforts to address the challenges facing both individuals and our healthcare system. In this paper, we jointly used artificial intelligence (AI) and participatory modeling to examine the possible consequences of this paradigm shift. Specifically, we created a conceptual map with 19 experts to understand how obesity and physical and mental well-being connect to each other and other factors. Three analyses were performed. First, we analyzed the factors that directly connect to obesity and well-being, both in terms of causes and consequences. Second, we created a reduced version of the map and examined the connections between categories of factors (e.g., food production, and physiology). Third, we explored the themes in the interviews when discussing either well-being or obesity. Our results show that obesity was viewed from a medical perspective as a problem, whereas well-being led to broad and diverse solution-oriented themes. In particular, we found that taking a well-being perspective can be more comprehensive without losing the relevance of the physiological aspects that an obesity-centric perspective focuses on.
Comprehensive Cardiovascular Management of Myotonic Dystrophy Type 1 Patients: A Report from the Italian Neuro-Cardiology Network
Authors: Russo Vincenzo , Antonini Giovanni , Massa Roberto , Casali Carlo , Mauriello Alfredo , Martino Anna , Marconi Roberto , Garibaldi Matteo , Franciosa Pasquale , Zecchin Massimo , Gaudio Carlo , D’Andrea Antonello , Strano Stefano
Published: February 2024

Myotonic dystrophy is a hereditary disorder with systemic involvement. The Italian Neuro-Cardiology Network-“Rete delle Neurocardiologie” (INCN-RNC) is a unique collaborative experience involving neurology units combined with cardio-arrhythmology units. The INCN facilitates the creation of integrated neuro-cardiac teams in Neuromuscular Disease Centers for the management of cardiovascular involvement in the treatment of myotonic dystrophy type 1 (MD1).
Paroxysmal Dystonic Posturing Mimicking Nocturnal Leg Cramps as a Presenting Sign in an Infant with DCC Mutation, Callosal Agenesis and Mirror Movements
Authors: Prato Adriana , Cirnigliaro Lara , Maugeri Federica , Luca Antonina , Giuliano Loretta , Vitiello Giuseppina , Errichiello Edoardo , Valente Enza Maria , Del Giudice Ennio , Mostile Giovanni , Rizzo Renata , Barone Rita
Published: February 2024

Background/Objectives: Pathogenic variants in the deleted in colorectal cancer gene (DCC), encoding the Netrin-1 receptor, may lead to mirror movements (MMs) associated with agenesis/dysgenesis of the corpus callosum (ACC) and cognitive and/or neuropsychiatric issues. The clinical phenotype is related to the biological function of DCC in the corpus callosum and corticospinal tract development as Netrin-1 is implicated in the guidance of developing axons toward the midline. We report on a child with a novel inherited, monoallelic, pathogenic variant in the DCC gene. Methods: Standardized measures and clinical scales were used to assess psychomotor development, communication and social skills, emotional and behavioural difficulties. MMs were measured via the Woods and Teuber classification. Exome sequencing was performed on affected and healthy family members. Results: The patient’s clinical presentation during infancy consisted of paroxysmal dystonic posturing when asleep, mimicking nocturnal leg cramps. A brain magnetic resonance imaging (MRI) showed complete ACC. He developed typical upper limb MMs during childhood and a progressively evolving neuro-phenotype with global development delay and behavioural problems. We found an intrafamilial clinical variability associated with DCC mutations: the proband’s father and uncle shared the same DCC variant, with a milder clinical phenotype. The atypical early clinical presentation of the present patient expands the clinical spectrum associated with DCC variants, especially those in the paediatric age. Conclusions: This study underlines the importance of in-depth genetic investigations in young children with ACC and highlights the need for further detailed analyses of early motor symptoms in infants with DCC mutations.
Chronic Hypertension in Pregnancy and Placenta-Mediated Complications Regardless of Preeclampsia
Authors: Cohen Yair , Gutvirtz Gil , Avnon Taeer , Sheiner Eyal
Published: February 2024

Background: The prevalence of chronic hypertension in women of reproductive age is on the rise mainly due to delayed childbearing. Maternal chronic hypertension, prevailing prior to conception or manifesting within the early gestational period, poses a substantial risk for the development of preeclampsia with adverse maternal and fetal outcomes, specifically as a result of placental dysfunction. We aimed to investigate whether chronic hypertension is associated with placenta-mediated complications regardless of the development of preeclampsia in pregnancy. Methods: This was a population-based, retrospective cohort study from ‘Soroka’ university medical center (SUMC) in Israel, of women who gave birth between 1991 and 2021, comparing placenta-mediated complications (including fetal growth restriction (FGR), placental abruption, preterm delivery, and perinatal mortality) in women with and without chronic hypertension. Generalized estimating equation (GEE) models were used for each outcome to control for possible confounding factors. Results: A total of 356,356 deliveries met the study’s inclusion criteria. Of them, 3949 (1.1%) deliveries were of mothers with chronic hypertension. Women with chronic hypertension had significantly higher rates of all placenta-mediated complications investigated in this study. The GEE models adjusting for preeclampsia and other confounding factors affirmed that chronic hypertension is independently associated with all the studied placental complications except placental abruption. Conclusions: Chronic hypertension in pregnancy is associated with placenta-mediated complications, regardless of preeclampsia. Therefore, early diagnosis of chronic hypertension is warranted in order to provide adequate pregnancy follow-up and close monitoring for placental complications, especially in an era of advanced maternal age.
Parental Competences and Stress Levels in Mothers of Children with Autism Spectrum Disorders and Children Developing Neurotypically
Authors: Tyszkiewicz-Gromisz Beata , Burdzicka-Wołowik Joanna , Tymosiewicz Piotr , Gromisz Wilhelm
Published: February 2024

(1) Background: the aim of this study was to explore parental competences and stress levels in the mothers of children with autism in relation to the mothers of neurotypical children. (2) Methods: the study used the Parental Competence Test and the PSS-10 scale to assess the intensity of stress related to one’s own life situation over the past month. Forty mothers of children with ASD (n = 20) and neurotypical children (n = 20) participated in the study. (3) Results: the mothers of children with ASD showed higher levels of stress (p = 0.0002). The mothers of neurotypical children achieved higher scores in parental competences (r = −0.49). The competence of mothers of children with ASD was correlated with rigour (r = 0.50), permissiveness (r = −0.60), overprotectiveness (r = 0.71), and helplessness (r = −0.77). (4) Conclusions: mothers of children with autism demonstrate lower parental competences than mothers of neurotypical children. Mothers of children with autism are less rigorous but more permissive, overprotective, and helpless. They tend to become heavily involved with their child. An overprotective attitude and greater tolerance for antisocial behaviours among parents of children with ASDs protect them from excessive stress.