bluuebunny/crossref_metadata_2025 huggingface dataset:
Smart Cities: Data-Driven Solutions to Understand Disruptive Problems in Transportation—The Lisbon Case Study
Authors: Albuquerque Vitória , Oliveira Ana , Barbosa Jorge Lourenço , Rodrigues Rui Simão , Andrade Francisco , Dias Miguel Sales , Ferreira João Carlos
Published: May 2021

Transportation data in a smart city environment is increasingly becoming available. This data availability allows building smart solutions that are viewed as meaningful by both city residents and city management authorities. Our research work was based on Lisbon mobility data available through the local municipality, where we integrated and cleaned different data sources and applied a CRISP-DM approach using Python. We focused on mobility problems and interdependence and cascading-effect solutions for the city of Lisbon. We developed data-driven approaches using artificial intelligence and visualization methods to understand traffic and accident problems, providing a big picture to competent authorities and supporting the city in being more prepared, adaptable, and responsive, and better able to recover from such events.
Renewable Energy Producers’ Strategies in the Visegrád Group Countries
Authors: Sulich Adam , Sołoducho-Pelc Letycja
Published: May 2021

Companies that belong to the energy sector can use Sustainable Development Goals (SDGs) for their strategies and diversify electrical energy production with reverence to the natural environment. This article aims to analyze sustainability strategy types among the Visegrád Group (V4) countries’ energy producers, who decided to generate electrical energy from the renewable resources. This research uses an inductive inference approach supported by a literature study and deductive reasoning supported by a statistical reference method. The main finding is that the energy producers from the V4 group have a common direction of evolution in their strategies. This change is based on a growing share of renewable energy sources to achieve environmental excellence strategies. The lack of renewable energy sector organizations’ strategies translates into disappointment with the goals pursued by these organizations. The significance of this study lies in an explanation of how sustainability strategies compare at a firm and country-level in a proposed classification. The analysis can open future research areas to examine development of strategies in the renewable energy sector.
The Security of Energy Supply from Internal Combustion Engines Using Coal Mine Methane—Forecasting of the Electrical Energy Generation
Authors: Borowski Marek , Życzkowski Piotr , Zwolińska Klaudia , Łuczak Rafał , Kuczera Zbigniew
Published: May 2021

Increasing emissions from mining areas and a high global warming potential of methane have caused gas management to become a vital challenge. At the same time, it provides the opportunity to obtain economic benefits. In addition, the use of combined heat and power (CHP) in the case of coalbed methane combustion enables much more efficient use of this fuel. The article analyses the possibility of electricity production using gas engines fueled with methane captured from the Budryk coal mine in Poland. The basic issue concerning the energy production from coalbed methane is the continuity of supply, which is to ensure the required amount and concentration of the gas mixture for combustion. Hence, the reliability of supply for electricity production is of key importance. The analysis included the basic characterization of both the daily and annual methane capture by the mine’s methane drainage system, as well as the development of predictive models to determine electricity production based on hourly capture and time parameters. To forecast electricity production, predictive models that are based on five parameters have been adopted. Models were prepared based on three time variables, i.e., month, day, hour, and two values from the gas drainage system-capture and concentration of the methane. For this purpose, artificial neural networks with different properties were tested. The developed models have a high value of correlation coefficient. but showed deviations concerning the very low values persisting for a short time. The study shows that electricity production forecasting is possible, but it requires data on many variables that directly affect the production capacity of the system.
Piezoresistive Sensing Approaches for Structural Health Monitoring of Polymer Composites—A Review
Authors: Nauman Saad
Published: May 2021

Structural health monitoring (SHM) is an emerging paradigm of real-time in situ structural evaluation for the detection of damage and structural degradation. This is achieved while the structure is kept in-service as against traditional non-destructive evaluation (NDE) techniques which require scheduled interventions while the structure is kept offline. SHM offers great advantages over traditional regimens of condition monitoring (CM) by improving structural reliability and safety through timely detection of structural defects also known as “diagnosis”. Polymeric composite materials offer the unique opportunity of integrating different phases for designing self-sensing smart systems capable of self-diagnosis. Polymers are unique in the sense that they can be designed in various configurations as they generally have facile manufacturing procedures. Among other properties, piezoresistance is the one that can be detected in composites in real-time as a function of strain. Conductive polymers including intrinsic and extrinsic conductive polymers can be used to induce piezoresistivity in composites. Careful design procedures can be adopted to maximize the sensitivity of these piezoresistive composites in order to fully exploit the potential of this property for SHM. Various manufacturing/integration strategies can be employed to effectively use piezoresistance in composites for structural health monitoring. These include self-sensing in carbon fiber-reinforced composites, use of surface deposited/mounted sensing films and patterns, integration of filaments and yarns during reinforcement manufacturing or lay-up and impregnation of reinforcements with piezoresistive matrices. A comprehensive review of these techniques is presented with the view of their utility in the SHM of composites. A selection criterion for these techniques is also presented based on sensitivity, manufacturing method and detection capability.
Plantations of Cinnamomum camphora (Linn) Presl with Distinct Soil Bacterial Communities Mitigate Soil Acidity within Polluted Locations in Southwest China
Authors: Chen Zhan , Maltz Mia R. , Zhang Yuguang , O’Brien Brendan J. , Neff Monica , Wang Yihao , Cao Jixin
Published: May 2021

Although the underlying mechanisms remain unknown, soils from different forest types exhibit distinct acidification-buffering capacities. We investigated soil properties and bacterial communities across five plantation types and different soil horizons in a severely acid-polluted site in Southwest China and evaluated the potential mechanisms driving differences in community structure. Soils collected from pure stands of Pinus massoniana Lamb.or Schima superba Gardn. et Champ. or mixed stands of these species showed the lowest soil pH and greater abundances of acid-producing and acidophilic taxa (Halanaerobiales and Rhodospirillales). Soils from pure stands of Cinnamomum camphora (Linn) Presl, or a mixture of C. camphora and P. massoniana, yielded the greatest concentrations of exchangeable calcium (Ca) and magnesium (Mg) and greater soil pH. Increased relative abundances of beneficial taxa may have contributed to soil aggregate formation (e.g., Bradyrhizobium canariense and Terracidiphilus sp.) and heightened environmental stress resistance (e.g., Gaiellales). Fewer acid-producing and acidophilic taxa found in soils associated with C. camphora suggest that planting C. camphora may help soils recover from acidification, while planting S. superba may not be as beneficial. Our findings illustrate how differences in soil microbial communities may impact soil-acidification-buffering capacity across different forest types, which have important implications for understanding environmental functions within the context of microbial diversity.
Effect of Plant Growth Regulators on Protease Activity in Forest Floor of Norway Spruce Stand
Authors: Holik Ladislav , Volánek Jiří , Vranová Valerie
Published: May 2021

Soil proteases are involved in organic matter transformation processes and, thus, influence ecosystem nutrient turnovers. Phytohormones, similarly to proteases, are synthesized and secreted into soil by fungi and microorganisms, and regulate plant rhizosphere activity. The aim of this study was to determine the effect of auxins, cytokinins, ethephon, and chlorocholine chloride on spruce forest floor protease activity. It was concluded that the presence of auxins stimulated native proteolytic activity, specifically synthetic auxin 2-naphthoxyacetic acid (16% increase at added quantity of 5 μg) and naturally occurring indole-3-acetic acid (18%, 5 μg). On the contrary, cytokinins, ethephon and chlorocholine chloride inhibited native soil protease activity, where ethephon (36% decrease at 50 μg) and chlorocholine chloride (34%, 100 μg) showed the highest inhibitory effects. It was concluded that negative phytohormonal effects on native proteolytic activity may slow down organic matter decomposition rates and hence complicate plant nutrition. The study enhances the understanding of rhizosphere exudate effects on soil microbial activity and soil nitrogen cycle.
ALDH2 p.E504K Variation and Sex Are Major Factors Associated with Current and Quitting Alcohol Drinking in Japanese Oldest Old
Authors: Sasaki Takashi , Nishimoto Yoshinori , Hirata Takumi , Abe Yukiko , Takebayashi Toru , Arai Yasumichi
Published: May 2021

This study identified the factors associated with current and quitting alcohol drinking in the Oldest Old to better understand the associated factors and mechanisms underlying drinking behaviors in this age group. Results of a questionnaire for drinking behavior in 1015 Japanese Oldest Old citizens aged 85 to 89 years revealed that 56.0% of men and 24.0% of women were current drinkers. A genome-wide association study revealed that the rs671 G > A variation, which corresponds to the aldehyde dehydrogenase 2 (ALDH2) p.E504K missense variant, was significantly associated with current drinking (odds ratio: 3.8, p = 3.33 × 10−31). Variable selection with 41 factors and multivariate regression logistic analysis for current drinking indicated that the rs671 genotype and sex were the most significant factors in the Oldest Old. Further analysis revealed that the rs671 genotype, alcohol-associated biomarkers, a history of heart or kidney disease, and frailty score are factors associated with quitting drinking in the Oldest Old men, whereas smoking history, walking time, and depression score were factors associated with quitting drinking in the Oldest Old women. These results indicate that the ALDH2 p.E504K variation is a major factor associated with current and quitting drinking in the Japanese Oldest Old.
Hydroacoustic Observations of Two Contrasted Seismic Swarms along the Southwest Indian Ridge in 2018
Authors: Ingale Vaibhav Vijay , Bazin Sara , Royer Jean-Yves
Published: May 2021

In 2018, two earthquake swarms occurred along spreading ridge segments of the ultra-slow Southwest Indian Ridge (SWIR). The first swarm was located at the spreading-ridge intersection with the Novara Fracture Zone, comprising 231 events (ISC catalogue) and spanning over 6 days (10 July to 15 July). The second swarm was more of a cluster of events focusing near a discontinuity, 220 km west of the Rodrigues Triple Junction, composed of 92 events and spanning over 31 days (27 September to 27 October). We examined these two swarms using hydroacoustic records from the OHASISBIO network with seven to nine autonomous hydrophones moored on either side of the SWIR. We detected 1109 hydroacoustic events spanning over 13 days (6 July to 18 July) in the first swarm and 4880 events spanning over 33 days in the second swarm (25 September to 27 October). The number of events per day was larger, and the hydroacoustic magnitude (source level) was, on average, smaller during the second swarm than the first. The spatio-temporal distribution of events from both swarms indicates a magmatic origin initiated by dike intrusions and followed by a readjustment of stresses in the surrounding crust.
Chemical Characterization of Particulate Matter in the Renaissance City of Ferrara
Authors: Marrocchino Elena , Telloli Chiara , Rizzo Antonietta
Published: May 2021

Atmospheric aerosols are today a key issue in air pollution, mostly related to public health. Two test areas in Ferrara, one in the city center (urban location) and one in the industrial area (industrial location), were studied in June–July 2016 using the SEM technique to identify the environmental impact of some potential pollutant sources. Collection was performed using adhesive tapes applied on the surface of road signs, which allows to select particulate matter moving on air with diffusion movement and to exclude the particles usually deposed by the gravitational process. Dimensional characterization has shown that, usually, smaller particles tend to aggregate themselves in bigger polycrystalline particles with the geometric diameter of up to 10 μm. Micro-analytical data have revealed a wide heterogeneous range of compositions: more abundant silicate followed by carbonate, chlorine, sulphate, carbon, and organic. This preliminary study has highlighted that the Renaissance city of Ferrara is affected by an environmental problem linked to the presence of particulate matter induced by industrial activities, as is the case with some of the most polluted cities in the world. The observations and analytical data pointed out the need for further investigation to better define the features of the fine particulate matter. This will be useful to preserve the cultural heritage of this Medieval-Renaissance city.
Fanconi Syndrome Leading to Hypophosphatemic Osteomalacia Related to Tenofovir Use
Authors: Rao Mana , Dadey Liam , Glowa Thomas , Veldkamp Peter
Published: May 2021

Tenofovir disoproxil fumarate (TDF) is used worldwide to treat and prevent Human Immunodeficiency Virus (HIV) infection. Fanconi syndrome is a complication of TDF use and is characterized by inadequate reabsorption of glucose, phosphate and protein in the proximal tubule of the kidney which may eventually lead to osteomalacia manifested by symptoms of pain, muscular weakness and difficulty ambulating. We present a patient with severe osteomalacia due to progressive and unrecognized Fanconi’s syndrome, who responded rapidly to TDF withdrawal, oral phosphate repletion and calcitriol. With the widespread use of TDF-containing antiviral regimens, it is critically important that physicians adhere to screening recommendations to detect early Fanconi syndrome, and recognize symptoms of osteomalacia as a serious complication.
Dental Education in an Era of COVID-19: Kuwait’s Experience
Authors: Baskaradoss Jagan Kumar , Al-Asfour Adel
Published: May 2021

The coronavirus disease 2019 (COVID-19) pandemic has had a significant impact on dental education worldwide. Due to the rapid spread of COVID-19 across Kuwait, it was essential for the Faculty of Dentistry (FoD) at Kuwait University to make appropriate modifications to the functioning of the dental school. The FoD’s goal was not only to ensure a safe environment for its staff, students, and patients but also to sustain the students’ academic progression. The FoD adopted several measures including the establishment of a COVID-19 response team, adoption of a blended-learning model, and phase-wise re-opening of the dental center. This paper discusses on the strategies that the FoD adopted, in response to the challenges posed by the pandemic.
Virtual 3D Campus for Universiti Teknologi Malaysia (UTM)
Authors: Salleh Syahiirah , Ujang Uznir , Azri Suhaibah
Published: May 2021

University campuses consists of many buildings within a large area managed by a single organization. Like 3D city modeling, a 3D model of campuses can be utilized to provide a better foundation for planning, navigation and management of buildings. This study approaches 3D modeling of the UTM campus by utilizing data from aerial photos and site observations. The 3D models of buildings were drawn from building footprints in SketchUp and converted to CityGML using FME software. The CityGML models were imported into a geodatabase using 3DCityDB and visualized in Cesium. The resulting 3D model of buildings was in CityGML format level of detail 2, consisting of ground, wall and roof surfaces. The 3D models were positioned with real-world coordinates using the geolocation function in SketchUp. The non-spatial attributes of the 3D models were also stored in a database managed by PostgreSQL. While the methodology demonstrated in this study was found to be able to create LoD2 building models. However, issues of accuracy arose in terms of building details and positioning. Therefore, higher accuracy data, such as point cloud data, should produce higher LoD models and accurate positioning.
An Unconventional Field Mapping Application: A Complete Opensource Workflow Solution Applied to Lithological Mapping of the Coatings of Cultural Heritage
Authors: Iandelli Niccolò , Coli Massimo , Donigaglia Tessa , Ciuffreda Anna Livia
Published: May 2021

The conservation of a primary importance historical building requires organization, management, continuous updating, comparison, and visualization of a large amount of data of different nature and origin. In relation to these aspects the use of a GIS brings various advantages including single and univocal management of the entire amount of existing data in a relational, dynamic, updatable and queryable way. The integration of a mobile solution permits the updating of the dataset and checking on site all information. The workflow presented uses opensource solutions, desktop and mobile, which allows the creation of an unconventional lithological Field Mapping activity: starting from photo interpretation and in situ survey, all the coating materials (stone, etc.) of some monuments of primary historical and cultural interest have been mapped (i.e., Duomo di Firenze, Duomo di Prato). The product can be considered as a lithological cartography, vertically oriented, processed by field surveys, geognostic surveys and photo interpretation. All combined to create a “lithological” mapping of the coatings of the various monuments; the advantage is a new approach for conservation and restoration of Cultural Heritage. The proposed workflow involves a mobile solution, opensource, that allows the verification and management of the database in the field.
Development of Multilayer Mesenchymal Stem Cell Cell Sheets
Authors: Ochiai Jun , Niihara Yutaka , Oliva Joan
Published: May 2021

Cell and gene therapies have been developing dramatically over the past decade. To face and adapt to the development of these new therapies, the Food and Drug Administration (FDA) wrote and updated new guidelines from 2016 and keep updating them. Mesenchymal stem cells (MSCs) are the most used cells for treatment, far ahead from the induced pluripotent stem cells (iPSCs), based on registered clinical trials at clinicaltrials.gov. They are widely used because of their differentiation capacity and their anti-inflammatory properties, but some controversies still require clear answers. Additional studies are needed to determine the dosage, the number, and the route of injections (location and transplantation method), and if allogenic MSCs are safe compared to autologous MSC injection, including their long-term effect. In this review, we summarize the research our company is conducting with the adipose stromal cells in engineering cell sheets and their potential application.
Analysis of the Unsteady Flow Field in a Steam Turbine Control Valve using Spectral Proper Orthogonal Decomposition
Authors: Windemuth Christian , Lange Martin , Mailach Ronald
Published: May 2021

A significant share of the conversion of thermal into electrical energy is realized by steam turbines. Formerly designed for continuous operation, today’s requirements include extended part load operation that can be accompanied by highly unstable flow conditions and vibrations within the control valve of the turbine. The prediction of the flow at part load conditions requires large computational efforts with advanced turbulence modeling in order to compute the flow at a reasonable accuracy. Due to the unsteadiness of the flow, the evaluation of the numerical results itself is a major challenge. The turbulent structures require statistical approaches, of which the use of Spectral Proper Orthogonal Decomposition (SPOD) has proven itself as a powerful method. Within this paper, the application of the method on a critical operating point with a temporal excitation of pressure oscillations observed in the experiments with dry air is presented. Using SPOD, the dominating flow phenomena were isolated and flow structures visualized.
A Joint Summarization and Pre-Trained Model for Review-Based Recommendation
Authors: Bai Yi , Li Yang , Wang Letian
Published: May 2021

Currently, reviews on the Internet contain abundant information about users and products, and this information is of great value to recommendation systems. As a result, review-based recommendations have begun to show their effectiveness and research value. Due to the accumulation of a large number of reviews, it has become very important to extract useful information from reviews. Automatic summarization can capture important information from a set of documents and present it in the form of a brief summary. Therefore, integrating automatic summarization into recommendation systems is a potential approach for solving this problem. Based on this idea, we propose a joint summarization and pre-trained recommendation model for review-based rate prediction. Through automatic summarization and a pre-trained language model, the overall recommendation model learns a fine-grained summary representation of the key content as well as the relationships between words and sentences in each review. The review summary representations of users and items are finally incorporated into a neural collaborative filtering (CF) framework with interactive attention mechanisms to predict the rating scores. We perform experiments on the Amazon dataset and compare our method with several competitive baselines. Experimental results show that the performance of the proposed model is obviously better than that of the baselines. Relative to the current best results, the average improvements obtained on four sub-datasets randomly selected from the Amazon dataset are approximately 3.29%.
Acute Toxicity Evaluation of Non-Innocent Oxidovanadium(V) Schiff Base Complex
Authors: Lima Lidiane M. A. , Murakami Heide , Gaebler D. Jackson , Silva Wagner E. , Belian Mônica F. , Lira Eduardo C. , Crans Debbie C.
Published: May 2021

The vanadium(V) complexes have been investigated as potential anticancer agents which makes it essential to evaluate their toxicity for safe use in the clinic. The large-scale synthesis and the acute oral toxicity in mice of the oxidovanadium(V) Schiff base catecholate complex, abbreviated as [VO(HSHED)dtb] containing a redox-active ligand with tridentate Schiff base (HSHED = N-(salicylideneaminato)-N’-(2-hydroxyethyl)-1,2-ethylenediamine) and dtb = 3,5-di-(t-butyl)catechol ligands were carried out. The body weight, food consumption, water intake as well biomarkers of liver and kidney toxicity of the [VO(HSHED)dtb] were compared to the precursors, sodium orthovanadate, and free ligand. The 10-fold scale-up synthesis of the oxidovanadium(V) complex resulting in the preparation of material in improved yield leading to 2–3 g (79%) material suitable for investigating the toxicity of vanadium complex. No evidence of toxicity was observed in animals when acutely exposed to a single dose of 300 mg/kg for 14 days. The toxicological results obtained with biochemical and hematological analyses did not show significant changes in kidney and liver parameters when compared with reference values. The low oral acute toxicity of the [VO(HSHED)dtb] is attributed to redox chemistry taking place under biological conditions combined with the hydrolytic stability of the oxidovanadium(V) complex. These results document the design of oxidovanadium(V) complexes that have low toxicity but still are antioxidant and anticancer agents.
Two New Haplotypes of Bartonella sp. Isolated from Lipoptena fortisetosa (Diptera: Hippoboscidae) in SE Poland
Authors: Bartosik Katarzyna , Maślanko Weronika , Buczek Alicja , Asman Marek , Witecka Joanna , Szwaj Ewelina , Błaszkiewicz Paweł Szczepan , Świsłocka Magdalena
Published: May 2021

Insects of the genus Lipoptena are parasitic arthropods with a broad host range. Due to the type of parasitism (hematophagy), their potential role as vectors of pathogens, i.e., Bartonella sp., Anaplasma phagocytophilum, Rickettsia spp., and Borrelia burgdorferi is considered. As the range of their occurrence has been changing dynamically in recent years and infestations of humans have increasingly been reported, these organisms are now the subject of numerous studies. Our research aimed to present the molecular characteristics of Bartonella sp. detected in Lipoptena fortisetosa parasitizing wild cervids in south-eastern Poland. Adults of Lipoptena spp. were collected from carcasses of roe deer and red deer between spring and autumn in 2013. The PCR method was used to detect Bartonella sp. in the insects. We report two new haplotypes of the rpoB gene of Bartonella sp. isolated from L. fortisetosa feeding on wild cervids in south-eastern Poland and the presence of this invasive ectoparasitic species in the studied area since 2013. Phylogenetic analyses of newly obtained Bartonella sp. haplotypes confirmed their unique position on the constructed tree and network topology. The rpoB gene sequences found belonging to lineage B support the view that this phylogenetic lineage represents a novel Bartonella species.
Future Directions in Maintenance Therapy in Multiple Myeloma
Authors: Holstein Sarah A. , Suman Vera J. , Hillengass Jens , McCarthy Philip L.
Published: May 2021

Autologous stem cell transplantation (ASCT) has been a backbone of therapy for newly diagnosed patients with multiple myeloma eligible for high-dose therapy for decades. Survival outcomes have continued to improve over time, in part because of the incorporation of highly effective induction regimens prior to ASCT as well as post-ASCT maintenance therapy. Randomized phase III clinical trials have helped establish lenalidomide maintenance as a standard of care. However, as nearly all patients will eventually experience disease relapse, there continues to be significant interest in developing novel maintenance strategies to improve upon lenalidomide maintenance. In this review, we summarize the available evidence for the use of immunomodulatory drugs, proteasome inhibitors, and monoclonal antibodies as post-ASCT maintenance therapies as well as discuss future directions and unanswered questions in the field.
Circulating Trimethylamine N-Oxide Is Associated with Increased Risk of Cardiovascular Mortality in Type-2 Diabetes: Results from a Dutch Diabetes Cohort (ZODIAC-59)
Authors: Flores-Guerrero Jose L. , van Dijk Peter R. , Connelly Margery A. , Garcia Erwin , Bilo Henk J. G. , Navis Gerjan , Bakker Stephan J. L. , Dullaart Robin P. F.
Published: May 2021

Trimethylamine N-oxide (TMAO), a novel cardiovascular (CV) disease and mortality risk marker, is a gut microbiota-derived metabolite as well. Recently, plasma concentrations of branched-chain amino acids (BCAA) have been reported to be affected by microbiota. The association of plasma TMAO with CV mortality in Type 2 Diabetes (T2D) and its determinants are still incompletely described. We evaluated the association between plasma BCAA and TMAO, and the association of TMAO with CV mortality in T2D individuals. We used data of 595 participants (mean age 69.5 years) from the Zwolle Outpatient Diabetes project Integrating Available Care (ZODIAC) cohort were analyzed. Plasma TMAO and BCAA were measured with nuclear magnetic resonance spectroscopy. CV mortality risk was estimated using multivariable-adjusted Cox regression models. Cross-sectionally, TMAO was independently associated with BCAA standardized (Std) β = 0.18 (95% Confidence Interval (CI) 0.09; 0.27), p <0.001. During a median follow-up of 10 years, 113 CV deaths were recorded. In Cox regression analyses, adjusted for multiple clinical and laboratory variables including BCAA, TMAO was independently associated with CV mortality: adjusted hazard ratio (adjHR) 1.93 (95% CI 1.11; 3.34), p = 0.02 (for the highest vs. the lowest tertile of the TMAO distribution). The same was true for analyses with TMAO as continuous variable: adjHR 1.32 (95% CI 1.07; 1.63), p = 0.01 (per 1 SD increase). In contrast, BCAAs were not associated with increased CV mortality. In conclusion, higher plasma TMAO but not BCAA concentrations are associated with an increased risk of CV mortality in individuals with T2D, independent of clinical and biochemical risk markers.
Indicators of Visual Prognosis in Diabetic Macular Oedema
Authors: Sen Sagnik , Ramasamy Kim , Sivaprasad Sobha
Published: May 2021

Diabetic macular oedema (DMO) is an important cause of moderate vision loss in people with diabetes. Advances in imaging technology have shown that a significant proportion of patients with DMO respond sub-optimally to existing treatment options. Identifying associations and predictors of response before treatment is initiated may help in explaining visual prognosis to patients and aid the development of personalized treatment strategies. Imaging features, such as central subfoveal thickness, photoreceptor integrity, disorganization of retinal inner layers, choroidal changes, and macular perfusion, have been reported to be prognostic factors of visual acuity (VA) in DMO. In this review we evaluated each risk factor to understand their relative importance in visual prognostication of DMO eyes post-treatment. Although individually, some of these factors may not be significant predictors, in combination they may form phenotypes that can inform visual prognosis. Stratification based on these phenotypes needs to be developed to progress to personalized medicine for DMO.
Dental Treatment Needs and Related Risk Factors among School Children with Special Needs in Taiwan
Authors: Hsiao Szu-Yu , Chen Ping-Ho , Huang Shan-Shan , Yen Cheng-Wei , Huang Shun-Te , Yin Shu-Yuan , Liu Hsiu-Yueh
Published: May 2021

The purpose of this study was to assess dental treatment needs (TNs) and related risk factors of children with disabilities (CD). This cross-sectional study recruited 484 CD, 6 to 12 years of age, from 10 special education schools in Taiwan. Dental status and TNs were examined and evaluated by well-trained dentists and based on the criteria set by the World Health Organization (1997). The results indicated that 61.78% required restorative dental treatment due to their dental caries. On average, each participant had 2.72 teeth that required treatment, and 6.38 surfaces required restoration. One-quarter of the participants (24.79%) required 1- or 2-surface restoration, and one out of three (36.98%) had more complex TNs (including 3 or more surfaces to be filled, pulp care, extraction, and more specialized care). The significant risk factors associated with restorative TNs among CD were those whose parents had lower socioeconomic status, frequent sweets intake, insufficient tooth-brushing ability, and poor oral health. Most of the CD had extensive unmet TNs for their caries and required complex treatment to recover the function of their teeth. Encouraging parents/caregivers to take their children for dental treatment, promoting awareness of the importance of dental hygiene, giving assistance to brushing their teeth after eating, and controlling and/or modifying sweet diet habits are necessary to reduce CD’s dental caries, especially those with lower socioeconomic status parents/caregivers.
Diagnosis of Sickle Cell Disease and HBB Haplotyping in the Era of Personalized Medicine: Role of Next Generation Sequencing
Authors: Adekile Adekunle , Akbulut-Jeradi Nagihan , Al Khaldi Rasha , Fernandez Maria , Sukumaran Jalaja
Published: May 2021

Hemoglobin genotype and HBB haplotype are established genetic factors that modify the clinical phenotype in sickle cell disease (SCD). Current methods of establishing these two factors are cumbersome and/or prone to errors. The throughput capability of next generation sequencing (NGS) makes it ideal for simultaneous interrogation of the many genes of interest in SCD. This study was designed to confirm the diagnosis in patients with HbSS and Sβ-thalassemia, identify any ß-thal mutations and simultaneously determine the ßS HBB haplotype. Illumina Ampliseq custom DNA panel was used to genotype the DNA samples. Haplotyping was based on the alleles on five haplotype-specific SNPs. The patients studied included 159 HbSS patients and 68 Sβ-thal patients, previously diagnosed using high performance liquid chromatography (HPLC). There was considerable discordance between HPLC and NGS results, giving a false +ve rate of 20.5% with a sensitivity of 79% for the identification of Sβthal. Arab/India haplotype was found in 81.5% of βS chromosomes, while the two most common, of the 13 β-thal mutations detected, were IVS-1 del25 and IVS-II-1 (G>A). NGS is very versatile and can be deployed to simultaneously screen multiple gene loci for modifying polymorphisms, to afford personalized, evidence-based counselling and early intervention.
Evolution of a Cohort of COVID-19 Infection Suspects Followed-Up from Primary Health Care
Authors: Coronado-Vázquez Valle , Ramírez-Durán Maria del Valle , Gómez-Salgado Juan , Dorado-Rabaneda María Silvia , Benito-Alonso Elena , Holgado-Juan Marina , Bronchalo-González Cristina
Published: May 2021

Diagnosis and home follow-up of patients affected by COVID-19 is being approached by primary health care professionals through telephone consultations. This modality of teleconsultation allows one to follow the evolution of patients and attend early to possible complications of the disease. The purpose of the study was to analyze the evolution of a cohort of patients with suspected SARS-CoV-2 disease followed by primary care professionals and to determine the factors that are associated with hospital admission. A prospective cohort study was carried out on 166 patients selected by consecutive sampling that showed symptoms compatible with COVID-19. The follow-up was approached via telephone for 14 days analyzing hospitalization and comorbidities of the patients. There were 75% of the hospitalized patients that were male (p = 0.002), and 70.8% presented comorbidities (p < 0.001). In patients with diabetes, the risk of hospitalization was 4.6-times larger, in hypertension patients it was 3.3-times, those suffering from renal insufficiency 3.8-times, and immunosuppressed patients 4.8-times (IC 95%: 1.9–11.7). In 86.7% of the cases, clinical deterioration was diagnosed in the first seven days of the infection, and 72% of healing was reached from day seven to fourteen. Monitoring from primary care of patients with COVID-19 allows early diagnosis of clinical deterioration and detection of comorbidities associated with the risk of poor evolution and hospital admission.
Significant Association of Polymorphisms in the TCF7L2 Gene with a Higher Risk of Type 2 Diabetes in a Moroccan Population
Authors: Elhourch Sarah , Arrouchi Housna , Mekkaoui Nour , Allou Younes , Ghrifi Fatima , Allam Loubna , Elhafidi Naima , Belyamani Lahcen , Ibrahimi Azeddine , Elomri Naoual , Eljaoudi Rachid
Published: May 2021

Background and aims: Several studies have shown that genetic polymorphisms of the transcription factor 7-like 2 (TCF7L2) are highly associated with the development of type 2 diabetes mellitus (T2DM) and its associated complications in several populations. The aim of our study was to investigate the association of the rs7903146 (C/T) and rs12255372 (G/T) polymorphism in the TCF7L2 gene with the risk of developing T2DM in the Moroccan population. Material and methods: A total of 150 T2DM patients and 100 healthy controls were recruited for various anthropometric, biochemical and genetic parameters. Genotyping was performed by using Real Time-PCR. The frequency of genotypes, alleles, anthropometric measures, glycemia, glycated hemoglobin (HbA1c) were evaluated in patients and control, while lipid profile was available only for T2DM group. Results: Glycemia, HbA1c and body mass index (BMI) were significantly higher in T2DM group than control. Analysis of the distribution of the TCF7L2 rs7903146 genotype and allele revealed that the TT genotype was more frequent in T2DM group (24.0%) than in healthy controls (5%) (OR = 4.08, 95% confidence interval (CI = 1.95–11.80, p < 0.0001). The T allele was more frequent in diabetic patients (45.2%) than healthy control (34.5%) and it was associated with high risk of diabetes (OR = 2.13, 95% CI = 1.12–7.31, p = 0.005). The same results were found regarding rs12255372, TT genotype frequencies were 18,7% and 6.0% in T2DM and control group, respectively (OR = 3.11, 95% CI = 1.33–7.24, p = 0.004). The T allele was over-presented in diabetics compared to controls (45.3% and 38.0%, respectively) and increases the risk of T2DM (OR = 2.01, 95% CI = 1.04–3.10, p = 0.01). However, there was no significant difference between the three genotypes of rs7903146 and rs12255372 regarding age, BMI, glycemia, HbA1c and lipid profile. Conclusion: The present study confirmed a significant association of the TCF7L2 gene (rs7903146 (C/T) and rs12255372 (G/T) polymorphisms with a higher risk to T2DM in the Moroccan population. No significant difference in respect to anthropometric and metabolic parameters between different genotypes.
How to Perceive the Trade-Off of Economic and Ecological Intensity of Land Use in a City? A Functional Zones-Based Case Study of Tangshan, China
Authors: Shen Wencang , Zhang Jianjun , Zhou Xiangli , Li Shengnan , Geng Xiaoli
Published: May 2021

China, in a rapid urbanization process, is accompanied by the expansion of built-up land, population accumulation, and intensive land investment, while the improvement of the urban environment cannot keep up with the population and economic density growth. Therefore, the purpose of this paper is to discuss the balance between urban land use economic intensity (built-up area density, population density, land price) and ecological intensity (the depth, breadth, and integration of ecological spaces) in Tangshan. From the perspective of functional zones (FZs), the trade-off of economic and ecological intensity of different types of land use is detailed from the evaluation system. Finally, this paper explores a common intensive development model for different FZs to reach both reasonable economic and ecological purposes in Tangshan. The upshot indicates that the economic and ecological intensity of all FZs in Tangshan follows an opposite spatial distribution, and different types of FZ own their characteristics. The Gini index and Theil index for measuring the typical FZ indicate the unbalanced state between ecological spaces and population in residential areas. To sum up, a resource-based city, such as Tangshan, in the rapid development stage of urbanization tends to pay more attention to economic output and neglect ecological benefits.
Advances in E-Health
Authors: Vimarlund Vivian , Koch Sabine , Nøhr Christian
Published: May 2021

E-health offers new ways to access health information, to deliver health and social care and to perform self-management [...]
Incorporation of an Allogenic Cortical Bone Graft Following Arthrodesis of the First Metatarsophalangeal Joint in a Patient with Hallux Rigidus
Authors: Brcic Iva , Pastl Klaus , Plank Harald , Igrec Jasminka , Schanda Jakob E. , Pastl Eva , Werner Mathias
Published: May 2021

Hallux rigidus is degenerative arthritis of the first metatarsophalangeal joint characterized by pain and stiffness in the joint with limitation of motion and functional impairment. Recently, bone grafts have been introduced in orthopedic procedures, namely osteosynthesis and arthrodesis. Allografts can induce bone formation, provide support for vascular and bone ingrowth and have a low risk of immunological rejection. A 52-year-old female patient with hallux rigidus underwent arthrodesis of the first metatarsophalangeal joint using Shark Screw® made of allogenic cortical bone. Corrective surgery was performed after 10 weeks, and a 5 × 3 mm large part of the Shark Screw® with the surrounding patient’s bone was removed. A histological evaluation revealed a vascularized graft with the newly formed compact lamellar bone fitting exactly to the cortical graft. The bone surface was lined by plump osteoblasts with osteoid production, and osteocytes were present in the lacunae. The arthrodesis of the first metatarsophalangeal joint using an allogenic cortical bone graft results in fast, primary bone healing without immunological rejection. This case suggests that the cortical allograft is a good and safe treatment option with an excellent graft incorporation into the host bone. However, as the literature evaluating the histology of different bone grafts is scarce, further high-level evidence studies with adequate sample sizes are needed to confirm our findings.
Ceftolozane/Tazobactam for Resistant Drugs Pseudomonas aeruginosa Respiratory Infections: A Systematic Literature Review of the Real-World Evidence
Authors: Giaccari Luca Gregorio , Pace Maria Caterina , Passavanti Maria Beatrice , Gargano Francesca , Aurilio Caterina , Sansone Pasquale
Published: May 2021

Background: Ceftolozane/tazobactam (C/T) is a β-lactam/β-lactamase inhibitor combination that mainly targets Gram-negative bacteria. The current international guidelines recommend including C/T treatment in the empirical therapy for hospital-acquired pneumonia (HAP) and ventilator-associated pneumonia (VAP). Pseudomonas aeruginosa (PA) is one of the most challenging Gram-negative bacteria. We conducted a systematic review of all cases reported in the literature to summarize the existing evidence. Methods: The main electronic databases were screened to identify case reports of patients with drug-resistant PA respiratory infections treated with C/T. Results: A total of 22 publications were included for a total of 84 infective episodes. The clinical success rate was 72.6% across a wide range of comorbidities. The 45.8% of patients treated with C/T presented colonization by PA. C/T was well tolerated. Only six patients presented adverse events, but none had to stop treatment. The most common therapeutic regimens were 1.5 g every 8 h and 3 g every 8 h. Conclusion: C/T may be a valid therapeutic option to treat multidrug-resistant (MDR), extensively drug-resistant (XDR), pandrug-resistant (PDR), and carbapenem-resistant (CR) PA infections. However, further data are necessary to define the optimal treatment dosage and duration.
The Effect of SARS-CoV-2 Virus Infection on the Course of Atopic Dermatitis in Patients
Authors: Miodońska Martyna , Bogacz Agnieszka , Mróz Magdalena , Mućka Szymon , Bożek Andrzej
Published: May 2021

Background and Objectives: Atopic dermatitis (AD) is a disease with a complex pathophysiology involving immune-mediated reactions that lead to skin lesions that are typically localized and recurrent. Following the outbreak of the COVID-19 (coronavirus disease 2019) pandemic, attempting to assess the impact of SARS-CoV-2 infection on diseases caused by complex immune mechanisms has become important. The aim of this study was to assess the impact of SARS-CoV-2 infection on the course of AD, including immunosuppressive therapy, in patients with a severe form of the disease. Materials and Methods: A retrospective analysis of 21 adults aged 18 to 52 years with AD diagnosed with COVID-19, including patients requiring hospitalization, was performed. Results: During SARS-CoV-2 infection, temporary exacerbation of skin lesions and/or skin pruritus was observed in nine (43%) patients but without the need for systemic treatment intervention. Patients with severe AD who received immunosuppressive therapy most often manifested mild exacerbation of skin symptoms. The skin condition improved in three patients. There was no significant effect of disease severity on the risk of severe COVID-19 (HR = 0.45; 95% CI: 0.32–0.65). Conclusions: The course of atopic dermatitis during SARS-CoV-2 infection may be different from the severity of its symptoms due to the lack of a significant influence. The immunosuppressive treatment used in patients with severe AD did not significantly affect the course of SARS-CoV-2 infection.
Entamoeba histolytica: Proteomics Bioinformatics Reveal Predictive Functions and Protein–Protein Interactions of Differentially Abundant Membrane and Cytosolic Proteins
Authors: Azmi Norhidayah , Othman Nurulhasanah
Published: May 2021

Amoebiasis is caused by Entamoeba histolytica and ranked second for parasitic diseases causing death after malaria. E. histolytica membrane and cytosolic proteins play important roles in the pathogenesis. Our previous study had shown several cytosolic proteins were found in the membrane fraction. Therefore, this study aimed to quantify the differential abundance of membrane and cytosolic proteins in membrane versus cytosolic fractions and analyze their predicted functions and interaction. Previous LC-ESI-MS/MS data were analyzed by PERSEUS software for the differentially abundant proteins, then they were classified into their functional annotations and the protein networks were summarized using PantherDB and STRiNG, respectively. The results showed 24 (44.4%) out of the 54 proteins that increased in abundance were membrane proteins and 30 were cytosolic proteins. Meanwhile, 45 cytosolic proteins were found to decrease in abundance. Functional analysis showed differential abundance proteins involved in the molecular function, biological process, and cellular component with 18.88%, 33.04% and, 48.07%, respectively. The STRiNG server predicted that the decreased abundance proteins had more protein–protein network interactions compared to increased abundance proteins. Overall, this study has confirmed the presence of the differentially abundant membrane and cytosolic proteins and provided the predictive functions and interactions between them.
Study of the Ecological Footprint and Carbon Footprint in a Reverse Osmosis Sea Water Desalination Plant
Authors: Leon Federico , Ramos-Martin Alejandro , Perez-Baez Sebastian Ovidio
Published: May 2021

The water situation in the Canary Islands has been a historical problem that has been sought to be solved in various ways. After years of work, efforts have focused on desalination of seawater to provide safe water mainly to citizens, agriculture, and tourism. Due to the high demand in the Islands, the Canary Islands was a pioneering place in the world in desalination issues, allowing the improvement of the techniques and materials used. There are a wide variety of technologies for desalination water, but nowadays the most used is reverse osmosis. Desalination has a negative part, the energy costs of producing desalinated water are high. To this we add the peculiarities of the electricity generation system in the Canary Islands, which generates more emissions per unit of energy produced compared to the peninsular generation system. In this study we have selected a desalination plant located on the island of Tenerife, specifically in the municipality of Granadilla de Abona, and once its technical characteristics have been known, the ecological footprint has been calculated. To do this we have had to perform some calculations such as the capacity to fix carbon dioxide per hectare in the Canary Islands, as well as the total calculation of the emissions produced in the generation of energy to feed the desalination plant.