bluuebunny/crossref_metadata_2025 huggingface dataset:
Does the timing of pasture allocation affect rumen and plasma metabolites and ghrelin, insulin and cortisol profile in dairy ewes?
Authors: Molle Giovanni , Gregorini Pablo , Cabiddu Andrea , Decandia Mauro , Giovanetti Valeria , Sitzia Maria , Dattena Maria , Porcu Cristian , Pasciu Valeria , Gonzalez-Bulnes Antonio , Berlinguer Fiammetta , Cannas Antonello
Published: September 2022

A study was undertaken to assess the impact of the timing of grazing on rumen and plasma metabolites and some metabolic hormones in lactating dairy sheep allocated to an Italian ryegrass (Lolium multiflorum Lam) pasture in spring for 4 h/d. Twenty-four mid lactation Sarda ewes stratified for milk yield, body weight, and body condition score, were divided into four homogeneous groups randomly allocated to the treatments (2 replicate groups per treatment). Treatments were morning (AM, from 08:00 to 12:00) and afternoon pasture allocation (PM, from 15:30 to 19:30). Samples of rumen liquor (day 39) and blood plasma (days 17 and 34 of the experimental period) were collected before and after the grazing sessions. Moreover, on days 11 and 35, grazing time was assessed by direct observation and herbage intake measured by the double weighing procedure. Grazing time was longer in PM than AM ewes (P < 0.001) but herbage intake was undifferentiated between groups. The intake of water-soluble carbohydrates at pasture was higher in PM than AM ewes (P < 0.05). The post-grazing propionic and butyric acid concentration, as measured on day 39, were higher in PM than AM ewes (P < 0.05). The basal level of glucose on day 34 and insulin (on both sampling days) were higher in PM than AM (P < 0.05). The opposite trend was detected for non-esterified fatty acids (P < 0.05, day 34) and urea (both days). Pasture allocation in the afternoon rather than in the morning decreased plasma concentration of ghrelin (P < 0.001) and cortisol (P < 0.001), with a smoothed trend on day 34 in the latter variable. To conclude, postponing the pasture allocation to afternoon increased the intake of WSC, favoring a glucogenic pattern of rumen fermentation and a rise of glucose and insulin levels in blood, although these effects were not consistent across the whole experimental period. Moreover, the afternoon grazing decreased the level of cortisol and ghrelin, suggesting a higher satiation-relaxing effect.
Producer practices and attitudes: Non-replacement male calf management in the Australian dairy industry
Authors: Vicic Veronika , Saliba Anthony J. , Campbell Michael A. , Xie Gang , Quinn Jane C.
Published: September 2022

Currently, there is no standardized rearing method or production guidelines for non-replacement male dairy calves that maximizes their economic viability. Producers have highlighted the need to match consumer expectations, but even with broadscale welfare improvement across the dairy industry, challenges remain at providing reliable and valuable pathways for non-replacement male dairy calves for beef production. A key consumer concern has been the use of on-farm euthanasia. Euthanasia has been a catalyst for change in the industry from a human and animal welfare perspective. The practice of euthanasia can lead to a decline in personnel wellbeing. To investigate the relationship between on-farm management practices of non-replacement male dairy calves and producer perceptions of their value proposition, an online questionnaire was provided to Australian dairy producers between June and October 2021. The aim was to identify supply-chain profitability of non-replacement male calves and investigate the attitudes and effects of euthanasia on producer wellbeing as part of managing these calves. A total of 127 useable responses were obtained, and a Bayesian network (BN) was utilized to model the interdependencies between management practices and wellbeing among participants. The results indicated that in general, dairy producers desired high welfare standards in their enterprises with regard to non-replacement male calves as well as expressed a desire to meet industry and consumers' expectations. In line with anecdotal reports of a reduction in practice, euthanasia was not identified as common practice in this group; however, producers were still accessing early-life markets for non-replacement male calves with operational requirements and environmental factors influencing their decisions. Producers expressed dissatisfaction with market access for their calves, as well as the lack of suitability of Australian beef grading standards for dairy-bred carcasses. Australian dairy managers and owners identified that euthanasia influenced employee wellbeing; however, they did not acknowledge euthanasia had an effect on their own wellbeing. Overall, the findings of this study indicate that all non-replacement male calf breeds had the potential to access profitable markets, and avoidance of euthanasia is a strong driver of change among dairy beef production systems in Australia.
Association of High Blood Pressure With Physical Activity, Screen-Based Sedentary Time, and Sedentary Breaks in a 2-Year Cohort of Community Dwelling Adults
Authors: Tebar William R. , Mielke Gregore Iven , Ritti-Dias Raphael M. , Silva Kelly Samara , Canhin Daniel S. , Scarabottolo Catarina C. , Mota Jorge , Christofaro Diego G. D.
Published: September 2022

Objective: This study analyzed the association of high blood pressure (HBP) with physical activity, sedentary behavior, and sedentary breaks in 2-year follow-up.

Methods: A sample of 331 middle-aged and older adults (mean age of 59.6 ± 17.3 years) was randomly selected and assessed at baseline and after 2 years of follow-up. HBP was considered as ≥140/90 mmHg values of systolic and diastolic blood pressure. Physical activity, sedentary behavior, and sedentary breaks were assessed by questionnaire. Age, sex, socioeconomic status, and body mass index were covariates.

Results: Continuous HBP was observed in 26.3% of sample between baseline and follow-up. Adults who reported continuously high sedentary breaks at leisure activities were less likely to have HBP (OR = 0.34, p = 0.011), as well as those who remained high physically active (OR = 0.41, p = 0.016), even after mutual adjustment. No association was observed between high sedentary behavior and HBP at follow-up.

Conclusion: Community dwelling adults who were high active and performed frequent sedentary breaks were less likely to have HBP in 2-year follow-up. Strategies for HBP control should considered both physical activities and leisure sedentary breaks in adult population.
PMSM Adaptive Sliding Mode Controller Based on Improved Linear Dead Time Compensation
Authors: Chen Huipeng , Yao Zhiqiang , Liu Yongqing , Lin Jian , Wang Peng , Gao Jian , Zhu Shaopeng , Zhou Rougang
Published: September 2022

Aiming at the issue of the sliding mode observer (SMO) being sensitive to the motor model parameters at low speeds, a rotor position observation method based on resistance adaptive SMO and considering the influence of inverter dead time is designed in this paper. In this method, the online resistance identification is introduced into the conventional SMO, the resistance parameters of the SMO are modified in real-time, and the online resistance identification algorithm is designed by using the Lyapunov function. An enhanced linear dead time compensation method is proposed to improve the resistance adaptive SMO and eliminate the voltage error between the estimated and the actual motor models in order to address the impact of inverter dead time on the voltage parameters in the estimation model at low speeds. Simulation results show that the online resistance identification and dead time compensation can significantly improve the accuracy of sensorless speed control at low speeds, and the dead time compensation can also improve the accuracy of online resistance identification.
Under Natural Field Conditions, Exogenous Application of Moringa Organ Water Extract Enhanced the Growth- and Yield-Related Traits of Barley Accessions
Authors: Tahir Nawroz Abdul-razzak , Lateef Djshwar Dhahir , Mustafa Kamil Mahmud , Rasul Kamaran Salh
Published: September 2022

Barley (Hordeum vulgare L.) is the preferred crop in arid regions, particularly for farmers with limited agricultural resources and low income. Typically, it is utilized for human consumption, animal feed, and malting. The discovery of natural (organic) sources of biostimulants has attracted a great deal of interest for crop productivity enhancement. Using a randomized complete block design with three main blocks, it was our aim to investigate the effects of foliar moringa (Moringa oleifera L.) organ extract (MOE) on the growth and yield components of a collection of barley accessions grown in Iraq. As indicated by the obtained results, almost all traits associated with barley growth and yield productivity were significantly enhanced by MOE application, relative to the respective control condition. The majority of barley accessions responded positively to the MOE treatment based on all studied traits (with the exception of 1000-kernel weight). According to the results of principal component analysis (PCA), the distribution of accessions on the two components under the MOE application was distinct from the distribution of accessions under control conditions, indicating that accessions responded differently to the MOE application. In addition, the distribution pattern of traits under MOE treatment was comparable to the distribution pattern of traits under the control condition, with the exception of two traits: total yield and 1000-kernel weight. AC5 and AC18 responded positively to the MOE application by possessing the highest total yield and harvest index values. The total yield trait registered the highest increasing value index (37.55%) based on the trait response index, followed by the straw weight (22.29%), tillering number per plant (21.44%), and spike number per plant (21.36%), while the spike length trait registered the lowest increasing value index (0.45%), compared to the traits under control conditions. So far, the results indicate that foliar application of MOE can be utilized effectively as a natural growth promoter to increase the growth and yield productivity of grown barley accessions.
Integrating Tillage and Mulching Practices as an Avenue to Promote Soil Water Storage, Growth, Production, and Water Productivity of Wheat under Deficit Irrigation in Arid Countries
Authors: Alsamin Bazel , El-Hendawy Salah , Refay Yahya , Tola ElKamil , Mattar Mohamed A. , Marey Samy
Published: September 2022

Ensuring food security with limited water resources in arid countries requires urgent development of innovative water-saving strategies. This study aimed to investigate the effects of various tillage and mulching practices on soil water storage (SWS), growth, production, irrigation water use efficiency (IWUE), and water productivity (WP) of wheat under full (FL) and limited (LM) irrigation regimes in a typical arid country. The tillage practices comprised the conventional tillage (CT) and reduced tillage (RT), each with five mulching treatments (MT), including non-mulched (NM), plastic film mulch (PFM), wheat straw mulch (WSM), palm residues mulch (PRM), and a mixture of wheat straw and palm residues at 50/50 ratio (MM). Results showed higher SWS at different measured time points in CT than RT at 20–40 cm, 40–60 cm, and 0–60 cm soil depth under FL regime, and at 40–60 cm under LM regime, while the opposite was observed at 0–20 cm and 20–40 cm soil depth under LM regime. SWS at different soil depths under MT, in most cases, followed the order of PFM > PRM ≈ MM > WSM > NM under FL, and PFM ≈ PRM > MM > WSM > NM under LM regimes. No significant differences were observed for traits related to growth between CT and RT, but RT increased the traits related to yield, IWUE, and WP by 5.9–11.6% than did CT. PFM and PRM or PRM and MM showed the highest values for traits related to growth or yield, IWUE, and WP, respectively. No significant differences in all traits between CT and RT under the FL regime were observed, however, RT increased all traits by 8.0–18.8% than did CT under the LM regime. The yield response factor (Ky) based on plant dry weight (KyPDW) and grain yield (KyGY) under RT was acceptable for four MT, while KyGY under CT was acceptable only for PRM, as the Ky values in these treatments were <1 under the LM regime. The interrelationships of plant dry weight (PDW), grain yield (GY), IWUE, and WP with evapotranspiration (ET), and of WP and IWUE with PDW and GY were best described by a second-order polynomial. SWS measured before irrigation exhibited strong linear relationships with PDW and GY (R2 range 0.57 to 0.92), while they exhibited a second order polynomial and moderate correlation with IWUE and WP (R2 range 0.29 to 0.54). Overall, combining RT with plant residue mulching, particularly using the readily available palm residues in sufficient amount is a feasible and sustainable water-saving strategy for enhancing wheat yield and WP in irrigated arid countries, such as Saudi Arabia.
Effect of Dietary Supplementation of Hydrolyzed Yeast on Growth Performance, Digestibility, Rumen Fermentation, and Hematology in Growing Beef Cattle
Authors: Gunun Nirawan , Sanjun Ittipol , Kaewpila Chatchai , Foiklang Suban , Cherdthong Anusorn , Wanapat Metha , Polyorach Sineenart , Khota Waroon , Kimprasit Thachawech , Kesorn Piyawit , Milintawisamai Nipa , Gunun Pongsatorn
Published: September 2022

This experiment was conducted to assess the effect of hydrolyzed yeast (HY) on growth performance, nutrient digestibility, rumen fermentation, and hematology in growing crossbred Bos indicus cattle. Twenty crossbred beef cattle with an initial body weight (BW) of 142 ± 12 kg were randomly assigned to one of four treatments for 90 d in a randomized complete block design (RCBD) having five blocks based on a homogenous subpopulation of sex and BW. Cattle were fed with a total mixed ration (TMR) and supplemented with HY at 0, 1, 2, and 3 g/kg dry matter (DM), respectively. Supplementation with the HY did not change average daily gain (ADG), dry matter intake (DMI), and gain to feed ratio (G:F) (p ≥ 0.06). The addition of HY did not adversely affect nutrient intake (p ≥ 0.48), while the digestibility of crude protein (CP) increased quadratically (p= 0.03) in the cattle receiving HY. The addition of HY did not affect rumen pH, but NH3-N concentration increased linearly (p = 0.02) in the cattle. The total volatile fatty acid (total VFA) increased quadratically (p= 0.03) when cattle were fed with HY supplementation. The proportion of acetate decreased cubically (p= 0.03) while propionate increased cubically (p= 0.01), resulting in a decrease in the acetate to propionate ratio (p= 0.01) when cattle were fed with HY supplementation. In addition, acetate was the lowest, but total VFA and propionate were the highest in cattle fed the HY at 2 g/kg DM. Butyrate increased cubically (p = 0.02) with the addition of HY. The protozoal and fungal populations were similar among treatments (p ≥ 0.11), but the bacterial population increased linearly (p < 0.01) with the addition of HY. Supplementation of HY did not influence blood urea nitrogen (BUN), red blood cells (RBC), hemoglobin, hematocrit, white blood cells (WBC), lymphocytes, or eosinophils (p≥ 0.10). However, monocytes and neutrophils increased linearly (p = 0.04 and p = 0.01, respectively) by HY supplementation. In conclusion, supplementation of HY at 2 g/kg DM promotes CP digestibility, rumen fermentation efficiency, and hematology but does not affect the growth performance of growing beef cattle.
Evaluation of Feed Near-Infrared Reflectance Spectra as Predictors of Methane Emissions from Ruminants
Authors: Sun Xuezhao , Pacheco David , Taylor Grant , Janssen Peter H. , Swainson Natasha M.
Published: September 2022

Feed chemical composition is associated with methane (CH4) formation in the rumen, and thus CH4 yields (Ym; CH4 emitted from per unit of dry matter intake) could be predicted using near-infrared reflectance spectroscopy (NIRS) of feeds fed to ruminants. Two databases of NIRS data were compiled from feeds used in experiments in which CH4 yields had been quantified in respiration chambers. Each record in the databases represented a batch of feed offered to a group of experimental animals and the mean CH4 yield for the group. A near-infrared reflectance spectrum was obtained from each feed, and these spectra were used to generate a predictive equation for Ym. The predictive model generated from brassica crops and pasture fed at a similar feeding level (n = 40 records) explained 53% of the variation in Ym and had a reasonably good agreement (concordance correlation coefficient of 0.77). The predictive ability of the NIRS calibration could be useful for screening purposes, particularly for predicting the potential Ym of multiple feeds or feed samples, rather than measuring Ym in animal experiments at high expenses. It is recommended that the databases for NIRS calibrations are expanded by collecting feed information from future experiments in which methane emissions are measured, using alternative algorithms and combining other techniques, such as terahertz time-domain spectroscopy.
Quercetin Is a Novel Inhibitor of the Choline Kinase of Streptococcus pneumoniae
Authors: Zimmerman Tahl , Ibrahim Salam A.
Published: September 2022

The effectiveness of current antimicrobial methods for addressing for food-borne Gram-positive pathogens has dropped with the emergence of resistant strains. Consequently, new methods for addressing Gram-positive strains have to be developed continuously. This includes establishing novel targets for antimicrobial discovery efforts. Eukaryotic choline kinases have been highly developed as drug targets for the treatment of cancer, rheumatoid arthritis, malaria and many other conditions and diseases. Recently, choline kinase (ChoK) has been proposed as a drug target for Gram-positive species generally. The aim of this work was to discover novel, natural sources of inhibitors for bacterial ChoK from tea extracts. We report the first natural bacterial ChoK inhibitor with antimicrobial activity against Streptococcus pneumoniae: quercetin.
Influence of Probiotic Strains Bifidobacterium, Lactobacillus, and Enterococcus on the Health Status and Weight Gain of Calves, and the Utilization of Nitrogenous Compounds
Authors: Zábranský Luboš , Poborská Anna , Gálik Branislav , Šoch Miloslav , Brož Petr , Kantor Martin , Kernerová Naděžda , Řezáč Ivan , Rolinec Michal , Hanušovský Ondrej , Strnad Ladislav , Havrdová Nikola
Published: September 2022

The aim of this study was to monitor the effect of Bifidobacterium bifidum (BB) and the combination of Lactobacillus sporogenes, Enterococcus faecium, and Bifidobacterium bifidum (LEB) on the health status and weight gain of calves, and the utilisation of nitrogenous substances. The experiment was performed in the period from April 2020 to September 2020. A total of 90 Holstein heifers, which were one to 56 days old, were used as experimental animals. Differences in live weight gain were significant if we compared the LEB vs. BB group and the LEB vs. C, the control group (86.23 ± 5.49 kg vs. 84.72 ± 6.22 kg, p < 0.05; 86.23 ± 5.49 kg vs. 82.86 ± 5.35 kg, p < 0.01). Considering the live weight gain, group BB was heavier than group C only (84.72 ± 6.22 kg vs. 82.86 ± 5.35 kg, p < 0.05). An effect on reducing the incidence and duration of diarrheal diseases was not demonstrated in this study (p = 0.1957). The administration of feed additives had no statistically significant effect on the amount of N excreted in the feces. The values of hematological and biochemical parameters were unaffected except for the first sampling of urea. Other blood parameters were not affected by the addition of probiotic feed additives. The bacterial populations in the feces 5 days and 56 days after birth were not affected by the inclusion of feed additives.
Carob (Ceratonia siliqua) as Functional Feed Is Beneficial in Yellow Mealworm (Tenebrio molitor) Rearing: Evidence from Growth, Antioxidant Status and Cellular Responses
Authors: Antonopoulou Efthimia , Panteli Nikolas , Feidantsis Kostantinos , Mastoraki Maria , Koutsogeorgiou Eleni , Grivaki Eirini , Papagrigoriou Theodora , Christias Spyros , Chatzifotis Stavros , Lazari Diamanto , Andreadis Stefanos , Krigas Nikos
Published: September 2022

In terms of sustainability and circular economy, agricultural by-products may be efficiently reused in insects’ rearing for high-quality protein sources in human diet and animal feeds. The present study aimed to explore whether the utilization of carob pods as feeding substrate may beneficially affect Tenebrio molitor’s growth, nutritional value, antioxidant status and cellular responses. Increasing levels of milled whole carob pods (0, 25, 50, 75, 100%) were used as alternative wheat bran (control) substrates for yellow mealworm rearing, while growth performance, proximate composition, total phenolic content, antioxidant enzyme activity and the expression of stress- and apoptotic-related proteins were evaluated in larvae. The results showed that carob pods’ content up to 75% did not significantly differentiate larvae weight, development time and total dry matter. Larvae total phenolic content and antioxidant activity exhibited a significant increase at 75% content. Although the antioxidant enzymes’ activity decreased at both 25 and 50% levels, higher carob content levels (75 and 100%) resulted in no significant changes compared to the control. Carob pods led to decreased apoptotic indicators and the low expression of most stress-related proteins compared to the control. The present findings demonstrate that carob pods and their antioxidant properties exert beneficial effects on T. molitor’s rearing and nutritional status, although 100% carob content may impact adversely the larvae due to the high amounts of carob tannins.
Design and Implementation of Cloud-Based Collaborative Manufacturing Execution System in the Korean Fashion Industry
Authors: Ko Minjae , Lee Changho , Cho Yongju
Published: September 2022

Recently, manufacturing companies have been improving quality and productivity, reducing costs, and producing customized products according to Industry 4.0. The global value chain (GVC) is also being reorganized and manufacturing companies are recovering the connectivity of value chains based on, e.g., the regional value chain (RVC) and reshoring. With the advent of Industry 4.0, many manufacturing companies are introducing smart factories. A new type of manufacturing execution system (MES), a core system of smart factories, is necessary, owing to the new technologies and the increase in collaboration between companies. Here, we present the framework, development, and application processes of a “cloud-based collaborative MES System” to support the value chain of “order-design-production-delivery” for the manufacture of personalized sportswear products in the fashion industry in Korea. To this end, first, nine future MES deployment directions and frameworks are presented. Second, we present the UML modeling, conceptual framework, and functional framework for MES system development, considering six future MES establishment directions such as cloud and collaboration. Third, the application and effect of the designed and developed cloud-based collaborative MES system are analyzed for design, fabric, printing, and sewing companies that play a role in each stage of the sportswear value chain.
Investigating the Energy-Efficient Structures Using Building Energy Performance Simulations: A Case Study
Authors: Abbas Safeer , Saleem Omer , Rizvi Mujasim Ali , Kazmi Syed Minhaj Saleem , Munir Muhammad Junaid , Ali Shahid
Published: September 2022

The use of energy efficient structures in the local construction industry assists in promoting green building concepts, leading to economical and eco-friendly solutions for self-sustained structures. The main aim of this study was to examine and compare the energy performance of various local buildings. Detailed 3D building models (house, office, and warehouse buildings) were constructed and investigated for their cost and energy savings using building energy simulation tools (green building studio and insight). Moreover, the effects of various building materials for walls, window panels, and roof construction were explored, and a life-cycle cost analysis was performed. It was observed that the effect of the window-to-wall ratio was less severe in term of energy use in office buildings compared to normal houses due to the larger amount of space available for air circulation. Furthermore, the most efficient location for windows was found to be at the middle of the wall in comparison with the top and bottom positions. The effect of the orientation mainly depended on the symmetry of the building. More symmetric buildings, i.e., tested warehouse buildings (rectangular structure), showed an energy use difference of around 7 MJ/m2/year for a 360° orientation change. Tested house buildings exhibited an energy use difference of up to 25 MJ/m2/year. Three-pane glass windows also showed major improvements, and the total energy consumption for houses was reduced to 14%. Furthermore, wood walls showed comparable energy performance with brick walls without the use of insulation. According to US-LEED guidelines, the tested house, office, and warehouse buildings achieved 79, 89, and 88 points, respectively. The cost recovery period for house, office, and warehouse buildings was estimated to 54, 13, and 14 years, respectively, including running and maintenance costs. It can be argued that the Insight and Green Building Studio packages can assist construction stakeholders to determine the energy efficiency of the modeled building as well as to help in the selection of materials for optimized and improved design.
Evading Logits-Based Detections to Audio Adversarial Examples by Logits-Traction Attack
Authors: Han Songshen , Xu Kaiyong , Guo Songhui , Yu Miao , Yang Bo
Published: September 2022

Automatic Speech Recognition (ASR) provides a new way of human-computer interaction. However, it is vulnerable to adversarial examples, which are obtained by deliberately adding perturbations to the original audios. Thorough studies on the universal feature of adversarial examples are essential to prevent potential attacks. Previous research has shown classic adversarial examples have different logits distribution compared to normal speech. This paper proposes a logit-traction attack to eliminate this difference at the statistical level. Experiments on the LibriSpeech dataset show that the proposed attack reduces the accuracy of the LOGITS NOISE detection to 52.1%. To further verify the effectiveness of this approach in attacking detection based on logits, three different features quantifying the dispersion of logits are constructed in this paper. Furthermore, a richer target sentence is adopted for experiments. The results indicate that these features can detect baseline adversarial examples with an accuracy of about 90% but cannot effectively detect Logits-Traction adversarial examples, proving that Logits-Traction attack can evade the logits-based detection method.
Understanding and Predicting the Usage of Shared Electric Scooter Services on University Campuses
Authors: Moosavi Seyed Mohammad Hossein , Ma Zhenliang , Armaghani Danial Jahed , Aghaabbasi Mahdi , Ganggayah Mogana Darshini , Wah Yuen Choon , Ulrikh Dmitrii Vladimirovich
Published: September 2022

Electric vehicles (EVs) have been progressing rapidly in urban transport systems given their potential in reducing emissions and energy consumptions. The Shared Free-Floating Electric Scooter (SFFES) is an emerging EV publicized to address the first-/last-mile problem in travel. It also offers alternatives for short-distance journeys using cars or ride-hailing services. However, very few SFFES studies have been carried out in developing countries and for university populations. Currently, many universities are facing an increased number of short-distance private car travels on campus. The study is designed to explore the attitudes and perceptions of students and staff towards SFFES usage on campus and the corresponding influencing factors. Three machine learning models were used to predict SFFES usage. Eleven important factors for using SFFESs on campus were identified via the supervised and unsupervised feature selection techniques, with the top three factors being daily travel mode, road features (e.g., green spaces) and age. The random forest model showed the highest accuracy in predicting the usage frequency of SFFESs (93.5%) using the selected 11 variables. A simulation-based optimization analysis was further conducted to discover the characterization of SFFES users, barriers/benefits of using SFFESs and safety concerns.
The Bridge and Narrativization of Vision: Ambrose Bierce and Vladimir Nabokov
Authors: Astvatsaturov Andrey
Published: September 2022

The article contains a comparative study of the visual poetics observed in the literary texts of American writer Ambrose Bierce and Russian-American writer Vladimir Nabokov. In particular, the study focuses on Bierce’s short story “An Occurrence at Owl Creek Bridge” and Nabokov’s three short stories “Details of a Sunset”, “Aurelian”, and “Perfection”, in all three of which a number of narrative tools, images, and motifs borrowed from Bierce’s text can be found. The representation of the bridge and the narrativization of mystical insight are regarded as the principal features of the correlative imagery systems. These features are analyzed in order to discover Bierce’s and Nabokov’s understandings of the artist, visual imagination, and the freedom of will.
Identification and Functional Analysis of the Cell Proliferation Regulator, Insulin-like Growth Factor 1 (IGF1) in Freshwater Pearl Mussel (Hyriopsis cumingii)
Authors: Li Xuenan , Feng Shangle , Wang He , Shen Xiaoya , Chen Yige , Xuan Xingrong , Fu Yuanshuai , Bai Zhiyi , Li Wenjuan
Published: September 2022

Insulin-like growth factor 1 (IGF1) plays an important regulatory role in the regulation of growth, differentiation, and anabolism in a variety of cells. In this study, the full-length cDNA of the IGF1 gene was cloned from Hyriopsis cumingii, named HcIGF1. The expression level of HcIGF1 in six tissues (adductor muscle, foot, hepatopancreas, gill, mantle, and gonad) was determined. In addition, the localization of HcIGF1 in the mantle was analyzed by in situ hybridization, and finally the function of HcIGF1 was explored by RNA interference and prokaryotic expression. The results showed that the amino acid sequence contained a typical IIGF structural domain. The phylogenetic tree showed that HcIGF1 clustered with other marine bivalve sequences. Quantitative real-time PCR and in situ hybridization analysis showed that HcIGF1 was expressed in all tissues. The highest expression was in the foot and the lowest was in the mantle. In the mantle tissue, the hybridization signal was mainly concentrated in the outer mantle. After RNA interference, the expression of IGF1 was found to be significantly decreased (p < 0.05), and its related genes IGF1R, AKT1, and cyclin D2 were downregulated, while MAPK1 were upregulated. The recombinant HcIGF1 protein was purified and its growth-promoting effect was investigated. The results showed that the recombinant HcIGF1 protein could significantly promote the proliferative activity of the mantle cells of mussels, with the best proliferative effect at 12.5 μg/mL. The results of this study provide a new method to solve the problem of weak proliferation of shellfish cells in vitro and lay the foundation for further understanding of the growth regulation mechanism of H. cumingii, as well as a better understanding of the physiological function of IGF1 in mollusks.
Exploring Biomolecular Self-Assembly with Far-Infrared Radiation
Authors: Kawasaki Takayasu , Yamaguchi Yuusuke , Kitahara Hideaki , Irizawa Akinori , Tani Masahiko
Published: September 2022

Physical engineering technology using far-infrared radiation has been gathering attention in chemical, biological, and material research fields. In particular, the high-power radiation at the terahertz region can give remarkable effects on biological materials distinct from a simple thermal treatment. Self-assembly of biological molecules such as amyloid proteins and cellulose fiber plays various roles in medical and biomaterials fields. A common characteristic of those biomolecular aggregates is a sheet-like fibrous structure that is rigid and insoluble in water, and it is often hard to manipulate the stacking conformation without heating, organic solvents, or chemical reagents. We discovered that those fibrous formats can be conformationally regulated by means of intense far-infrared radiations from a free-electron laser and gyrotron. In this review, we would like to show the latest and the past studies on the effects of far-infrared radiation on the fibrous biomaterials and to suggest the potential use of the far-infrared radiation for regulation of the biomolecular self-assembly.
Trends and Perspectives of Biological Drug Approvals by the FDA: A Review from 2015 to 2021
Authors: Martins Alexander C. , Oshiro Mariana Y. , Albericio Fernando , de la Torre Beatriz G. , Pereira Gustavo José V. , Gonzaga Rodrigo V.
Published: September 2022

Despite belonging to a relatively new class of pharmaceuticals, biological drugs have been used since the 1980s, when they brought about a breakthrough in the treatment of chronic diseases, especially cancer. They conquered a large space in the pipeline of the pharmaceutical industry and boosted the innovation portfolio and arsenal of therapeutic compounds available. Here, we report on biological drug approvals by the US Food and Drug Administration (FDA) from 2015 to 2021. The number of drugs included in this class grew over this period, totaling 90 approvals, with an average of 13 authorizations per year. This figure contrasts with previous periods, which registered between 2 and 8 approvals per year. We highlight the great potential and advantages of biological drugs. In this context, these therapeutics show high efficacy and high selectivity, and they have brought about a significant increase in patient survival and a reduction of adverse reactions. The development and production of biopharmaceuticals pose a major challenge because these processes require cutting-edge technology, thereby making the drugs very expensive. However, we believe that, in the near future, biological medicines will be more accessible and new drugs belonging to this class will become available as new technologies emerge. Such advances will enhance the production of these biopharmaceuticals, thereby making the process increasingly profitable and less expensive, thereby bringing about greater availability of these drugs.
Stress Hormone Corticosterone Controls Metabolic Mitochondrial Performance and Inflammatory Signaling of In Vitro Cultured Sertoli Cells
Authors: Silva Ana M. , Ribeiro Carina T. , Bernardino Raquel L. , Jarak Ivana , Carvalho Rui A. , Pereira-Sampaio M. A. , de Souza Diogo B. , Alves Marco G. , Oliveira Pedro F.
Published: September 2022

Stress, as a physiological response, is a major factor that affects several processes, including reproductive functions. The main hormonal players of stress are cortisol (humans) and corticosterone (rodents). Sertoli cells (SCs), as key contributors for the testicular homeostasis maintenance, are extensively challenged by different hormones, with glucocorticoid corticosterone being the signaling modulator that may impact these cells at different levels. We aimed to characterize how corticosterone modulates SCs energy balance, putting the mitochondrial performance and signaling output in perspective as the cells can disperse to the surroundings. TM4 mouse SCs were cultured in the absence and presence of corticosterone (in nM: 20, 200, and 2000). Cells were assessed for extracellular metabolic fluxes, mitochondrial performance (cell respirometry, mitochondrial potential, and mitochondrial complex expressions and activities), and the expression of androgen and corticosteroid receptors, as well as interleukine-6 (IL-6) and glutathione content. Corticosterone presented a biphasic impact on the extracellular fluxes of metabolites. Low sub-physiological corticosterone stimulated the glycolytic activity of SCs. Still, no alterations were perceived for lactate and alanine production. However, the lactate/alanine ratio was decreased in a dose-dependent mode, opposite to the mitochondrial complex II activity rise and concurrent with the decrease of IL-6 expression levels. Our results suggest that corticosterone finely tuned the energetic profile of mouse SCs, with sub-physiological concentrations promoting glycolytic expenditure, without translating into cell redox power and mitochondrial respiratory chain performance. Corticosterone deeply impacted the expression of the pro-inflammatory IL-6, which may alter cell-to-cell communication in the testis, in the last instance and impact of the spermatogenic performance.
Development of an Open Microfluidic Platform for Oocyte One-Stop Vitrification with Cryotop Method
Authors: Miao Shu , Guo Chenxi , Jiang Ze , Wei Hao-Xiang , Jiang Xin , Gu Jingkai , Hai Zhuo , Wang Tianren , Liu Yun-Hui
Published: September 2022

Oocyte vitrification technology is widely used for assisted reproduction and fertility preservation, which requires precise washing sequences and timings of cryoprotectant agents (CPAs) treatment to relieve the osmotic shock to cells. The gold standard Cryotop method is extensively used in oocyte vitrification and is currently the most commonly used method in reproductive centers. However, the Cryotop method requires precise and complex manual manipulation by an embryologist, whose proficiency directly determines the effect of vitrification. Therefore, in this study, an automatic microfluidic system consisting of a novel open microfluidic chip and a set of automatic devices was established as a standardized operating protocol to facilitate the conventional manual Cryotop method and minimize the osmotic shock applied to the oocyte. The proposed open microfluidic system could smoothly change the CPA concentration around the oocyte during vitrification pretreatment, and transferred the treated oocyte to the Cryotop with a tiny droplet. The system better conformed to the operating habits of embryologists, whereas the integration of commercialized Cryotop facilitates the subsequent freezing and thawing processes. With standardized operating procedures, our system provides consistent treatment effects for each operation, leading to comparable survival rate, mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) level of oocytes to the manual Cryotop operations. The vitrification platform is the first reported microfluidic system integrating the function of cells transfer from the processing chip, which avoids the risk of cell loss or damage in a manual operation and ensures the sufficient cooling rate during liquid nitrogen (LN2) freezing. Our study demonstrates significant potential of the automatic microfluidic approach to serve as a facile and universal solution for the vitrification of various precious cells.
Model of the SARS-CoV-2 Virus for Development of a DNA-Modified, Surface-Enhanced Raman Spectroscopy Sensor with a Novel Hybrid Plasmonic Platform in Sandwich Mode
Authors: Samodelova Mariia V. , Kapitanova Olesya O. , Meshcheryakova Nadezda F. , Novikov Sergey. M. , Yarenkov Nikita R. , Streletskii Oleg A. , Yakubovsky Dmitry I. , Grabovenko Fedor I. , Zhdanov Gleb A. , Arsenin Aleksey V. , Volkov Valentyn S. , Zavyalova Elena G. , Veselova Irina A. , Zvereva Maria I.
Published: September 2022

The recent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has posed a great challenge for the development of ultra-fast methods for virus identification based on sensor principles. We created a structure modeling surface and size of the SARS-CoV-2 virus and used it in comparison with the standard antigen SARS-CoV-2—the receptor-binding domain (RBD) of the S-protein of the envelope of the SARS-CoV-2 virus from the Wuhan strain—for the development of detection of coronaviruses using a DNA-modified, surface-enhanced Raman scattering (SERS)-based aptasensor in sandwich mode: a primary aptamer attached to the plasmonic surface—RBD-covered Ag nanoparticle—the Cy3-labeled secondary aptamer. Fabricated novel hybrid plasmonic structures based on “Ag mirror-SiO2-nanostructured Ag” demonstrate sensitivity for the detection of investigated analytes due to the combination of localized surface plasmons in nanostructured silver surface and the gap surface plasmons in a thin dielectric layer of SiO2 between silver layers. A specific SERS signal has been obtained from SERS-active compounds with RBD-specific DNA aptamers that selectively bind to the S protein of synthetic virion (dissociation constants of DNA-aptamer complexes with protein in the range of 10 nM). The purpose of the study is to systematically analyze the combination of components in an aptamer-based sandwich system. A developed virus size simulating silver particles adsorbed on an aptamer-coated sensor provided a signal different from free RBD. The data obtained are consistent with the theory of signal amplification depending on the distance of the active compound from the amplifying surface and the nature of such a compound. The ability to detect the target virus due to specific interaction with such DNA is quantitatively controlled by the degree of the quenching SERS signal from the labeled compound. Developed indicator sandwich-type systems demonstrate high stability. Such a platform does not require special permissions to work with viruses. Therefore, our approach creates the promising basis for fostering the practical application of ultra-fast, amplification-free methods for detecting coronaviruses based on SARS-CoV-2.
C–O Hydrogenolysis of C3–C4 Polyols Selectively to Terminal Diols over Pt/W/SBA-15 Catalysts
Authors: Bhowmik Susmita , Enjamuri Nagasuresh , Marimuthu Banu , Darbha Srinivas
Published: September 2022

Pt/W/SBA-15 catalysts (with Pt-loading = 0.5–4 wt% and W-loading = 1 wt%) prepared by the sequential impregnation method were evaluated for selective C–O cleavage of erythritol and glycerol in an aqueous medium. The Pt and W particles dispersed on SBA-15 approached close proximity at higher Pt loadings and afforded synergistic enhancement in C–O hydrogenolysis activity/selectivity. 1,4-Butanediol yields of 30.9% (at 190 °C, 50 bar H2 and 24 h) and 1,3-propanediol yields of 34.4% (at 190 °C, 50 bar H2 and 12 h of reaction) were obtained over these catalysts. Pt nanoparticles (facilitating dissociative H2 adsorption and spillover) and W (present as acidic oligomeric WOx species; activating and coordinating the polyol via 1°-OH group) worked in tandem for the selective hydrogenolysis of polyols yielding terminal diols of industrial demand.
Multi-Strain Probiotic Mixture Affects Brain Morphology and Resting State Brain Function in Healthy Subjects: An RCT
Authors: Rode Julia , Edebol Carlman Hanna M. T. , König Julia , Hutchinson Ashley N. , Thunberg Per , Persson Jonas , Brummer Robert J.
Published: September 2022

Probiotics can alter brain function via the gut–brain axis. We investigated the effect of a probiotic mixture containing Bifidobacterium longum, Lactobacillus helveticus and Lactiplantibacillus plantarum. In a randomized, placebo-controlled, double-blinded crossover design, 22 healthy subjects (6 m/16 f; 24.2 ± 3.4 years) underwent four-week intervention periods with probiotics and placebo, separated by a four-week washout period. Voxel-based morphometry indicated that the probiotic intervention affected the gray matter volume of a cluster covering the left supramarginal gyrus and superior parietal lobule (p < 0.0001), two regions that were also among those with an altered resting state functional connectivity. Probiotic intervention resulted in significant (FDR < 0.05) functional connectivity changes between regions within the default mode, salience, frontoparietal as well as the language network and several regions located outside these networks. Psychological symptoms trended towards improvement after probiotic intervention, i.e., the total score of the Hospital Anxiety and Depression Scale (p = 0.056) and its depression sub-score (p = 0.093), as well as sleep patterns (p = 0.058). The probiotic intervention evoked distinct changes in brain morphology and resting state brain function alongside slight improvements of psycho(bio)logical markers of the gut–brain axis. The combination of those parameters may provide new insights into the modes of action by which gut microbiota can affect gut–brain communication and hence brain function.
Retinoids Promote Mouse Bone Marrow-Derived Macrophage Differentiation and Efferocytosis via Upregulating Bone Morphogenetic Protein-2 and Smad3
Authors: Fige Éva , Sarang Zsolt , Sós László , Szondy Zsuzsa
Published: September 2022

Clearance of apoptotic cells by bone marrow-derived macrophages differentiated from monocytes plays a central role in the resolution of inflammation, as the conversion of pro-inflammatory M1 macrophages to M2 macrophages that mediate the resolution process occurs during efferocytosis. Thus, proper efferocytosis is a prerequisite for proper resolution of inflammation, and failure in efferocytosis is associated with the development of chronic inflammatory diseases. Previous studies from our laboratory have shown that (13R)-all-trans-13,14-dihydroretinol (DHR), the product of retinol saturase, acting from day 4 of monocyte differentiation enhances the efferocytosis capacity of the resulted macrophages. Loss of retinol saturase in mice leads to impaired efferocytosis, and to development of autoimmunity. In the present paper, we report that in differentiating monocytes DHR, retinol, and all-trans retinoic acid all act directly on retinoic acid receptors and enhance the clearance of apoptotic cells by upregulating the expression of several efferocytosis-related genes. The effect of retinoids seems to be mediated by bone morphogenetic protein (BMP)-2, and the Smad3 transcription factor. In addition, retinoids also upregulate the expression of the vitamin D receptor and that of vascular endothelial growth factor A, indicating that altogether retinoids promote the generation of a pro-reparative M2 macrophage population during monocyte differentiation.