bluuebunny/crossref_metadata_2025 huggingface dataset:
The role of the IGAD in security and peacebuilding: The case of Somalia
Authors: Nansasi Victoria , Arslan Mustafa
Published: September 2024

In recent times, the significance attributed to regional organizations has been one of the essential elements in analyzing peace operations, peacekeeping, and coalition operations. Contemporary international governmental organizations across the globe have been established with the main mandate of preventing, managing and resolving conflicts within their areas of influence. Intergovernmental Authority on Development is one such regional organization. Since the 1990s, foreign engagement in civil wars, particularly through peacekeeping operations, has expanded dramatically. This has weakened the traditional concept of non-intervention in internal affairs established in the United Nations Charter, or at least reignited debate over its meaning. Inequalities among regional bodies with regional peacekeeping responsibilities, such as agreements in peace enforcement, came to be viewed as indisputably valid measures, frequently superior to operations carried out through the United Nations. Conceptually, it was natural to believe that these organizations, being closer to the problems of a region and the actors, and having greater control over military means, could manage these missions more effectively. The goal of this paper is to conduct a complete examination of the IGAD’s efficacy in coordinating security and reconciliation initiatives in Somalia, to assess IGAD’s strategic designs, activities, and their impact in promoting regional stability, and to identify the key obstacles and opportunities associated with strengthening IGAD’s capacity for conflict resolution and governance change across the Horn of Africa. In this context, this research based on qualitative methodology has found that IGAD played an active role in the Somali Peace, developed easily adaptable solutions to problems thanks to its flexible structure, and played an active role in drawing the attention of the international public to Somalia and providing financial support.
CHAPTER 10: Assessing the Preparedness of NCV Tourism Graduates: A Study on the Alignment of Curriculum and Industry Demands
Authors: Mkhize-Simelane Bianca Nolwandle
Published: September 2024

The purpose of this chapter is to report on the findings of a qualitative study that sought to assess the responsiveness of the National Certificate (Vocational) (NC(V) tourism curriculum to industry demands. The study was motivated by evidence of shortcomings in translating curriculum guidelines into effective classroom practices, including discrepancies in time allocation for workplace-based experiences and inadequate utilisation of recommended teaching resources. There appears to be an overemphasis on theoretical instruction at the expense of practical skills development.
Estudio bitemporal de la vegetación en los Llanos Centro-Orientales de Venezuela
Authors: Martinez Moreno Enrique , García Julián A. , Vilachá Víctor M. , Montes Urdaneta Gustavo
Published: September 2024

Mediante el análisis espacial de imágenes satelitales multiespectrales se estudiaron los cambios cualitativos y cuantitativos ocurridos entre abril de 1990 y enero de 2003 en la cobertura vegetal de un sector de los Llanos Centroorientales (52,111 ha) en el suroeste del estado Anzoátegui. La selección, procesamiento y análisis de imágenes de archivo o adquiridas; la producción y verificación de mapas de imágenes de vegetación a escalas muy detalladas; y la recolección e interpretación de información ecológica extraída de productos derivados de estos mapas se realizó de manera multidisciplinaria.
Se utilizaron técnicas de clasificación multitemporal utilizando imágenes de satélite de media resolución (Landsat), estableciendo como línea base abril de 1990 y comparándola con la situación de enero de 2003. Así, se detectaron y cuantificaron cambios en la vegetación entre ambas fechas. Además, se utilizaron imágenes satelitales de alta resolución (Ikonos) para validar áreas importantes de la imagen de enero de 2003, lo que permitió la producción de mapas detallados de la infraestructura actual. La inspección de campo incluyó un vuelo en helicóptero para verificar visualmente el resultado de la clasificación supervisada y obtener un registro fotográfico georreferenciado de las áreas que eran de especial interés para la verificación.
Palabras clave: Vetegación, imágenes Landsat, análisis multitemporal, detección de cambios, clasificación multiespacial
La investigación de la enfermería. Un punto de vista
Authors: Loza Murguía Manuel Gregorio
Published: September 2024

Una profesional de la enfermería, si analiza la evolución de la profesión, en el espectro laboral, académico, investigación y/o desde la gestora de estos procesos, no es ajena a los cambios que se están suscitando desde hace al menos 200 años. Seguramente cada periodo tuvo una singularidad propia, dando lugar a un sinfín de momentos caracterizados en cada época.
The Case of the Awgwan: Considering Ethics of Digitization and Access for Archives
Authors: Brink Peterson , Ducey Mary Ellen , Lorang Elizabeth
Published: September 2024

As members of the University of Nebraska–Lincoln (UNL) Libraries considered digitizing and presenting the campus humor magazine Awgwan online as part of efforts to chronicle the University’s history and invite study of these materials, questions emerged about the ethics and responsibility of placing the Awgwan online in a broadly accessible digital environment. Much of the magazine depended and traded upon negative and destructive depictions of women and people of color as a key part of its “humor.” What are our ethical obligations in treating such materials? This essay introduces and analyzes the Awgwan; considers the potential value in making the magazine broadly available electronically; and then explores some of the issues the magazine raises for practitioners in archives and special collections today, particularly as more institutions may consider placing similar materials from their collections online.
Assessing Archival Collections through Surveys
Authors: Pflug Wendy
Published: September 2024

Assessment of collections holdings through survey can be used as the first step in the larger goal to uncover archives and special collections materials that are backlogged and thus inaccessible to researchers. This article seeks to gather data on the experiences and outcomes of collections assessment surveys. The responses of thirty-three archives and special collections professionals were analyzed to discover whether a survey was an effective planning tool and to determine if similarities would emerged that could be widely applied to future collections assessments surveys.
Literary Manuscripts in the Classroom: Using Manuscript Collections to Engage Undergraduate Students
Authors: Hertenstein Libby
Published: September 2024

Special collections librarians need to find new ways to engage undergraduate students. One way to engage students is to approach faculty members with innovative ways to integrate special collections materials into their curriculum. At Bowling Green State University, the rare books librarian developed an outreach opportunity for undergraduate students to examine the literary process from manuscript to publication. Using the collection, she created packets for students that gave them the opportunity to track editorial changes. This article discusses the development and implementation of this project and ends with tips for others interested in using literary manuscripts in the classroom.
Playfulness in the Archives: Enhancing Digital Collections through Card Sorting
Authors: Passehl-Stoddart Erin
Published: September 2024

Regardless of institutional type or resources, one question facing archives and special collections is how archival collections can be efficiently enhanced with minimal or no original metadata. This issue becomes a focal point when collections are digitized, as metadata is what makes digital collections more accessible and usable. This case study explores the development of a digital collection using card sorting activities and gamification techniques, and analyzes the direct and indirect effects of each strategy, including student employee connections to library learning goals and visual literacy standards.
Evidence-Based Physiotherapy and Rehabilitation Practices in Cerebral Palsy
Authors: Apaydin Yasemin , Kirmizi Muge
Published: September 2024

Although a child with cerebral palsy (CP) has developmental problems, it is known that the main problems are related to mobility. The aim of physiotherapy and rehabilitation intervention is to prevent or minimize motor, cognitive, sensory impairments in infants with developmental disadvantage due to biological or environmental risk factors. Starting from infancy until adolescence, the goal is always to enable individuals with CP to be independent in daily life to the extent permitted by their functional capacity. The main goal in the treatment of mobile children is activities such as walking, standing and postural control. In order to achieve these goals, secondary disorders should be prevented and the musculoskeletal system should be in good condition. In more severely affected children, it is important to ensure postural control, prevent deformities and maximize independence in daily life. Another point to be considered in the treatment is that problems such as spasticity, range of motion, selective control affect the mobility, social function and self-care activities of the child. Many such parameters should be thoroughly evaluated and an appropriate treatment plan should be developed. Treatment should not focus on a single function. Collaboration with the family should be ensured. Environmental enrichment parameters should be prioritized. Elimination of environmental barriers also plays an important role in treatment. All family members should take an active role in the treatment and be involved in the goal-setting process. In this section, the recent physiotherapy and rehabilitation applications in the literature used in individuals with CP from the early period will be summarized.
Orthopedic Problems and Management in Cerebral Palsy
Authors: Yilmaz Nilsah , Eymir Musa
Published: September 2024

Orthopedic problems in cerebral palsy occur secondary to primary deficits. Primary deficits include impairment in position, abnormalities in muscle tone, imbalance and coordination disorders, decreased strength and loss of selective motor control. Secondary deficits include muscle contractures and bone deformities, leading to further motor dysfunction and the need for orthopedic surgery. Management of orthopaedic problems caused by contractures and deformities is achieved through conservative treatments, pharmacologic agents or surgery. Conservative treatment consists of occupational therapy and physical therapy. Pharmacologic treatment aims to provide benefit by affecting the neurological processes underlying orthopedic problems. Surgical methods are aimed at bone deformities and muscle contractures. All of these methods aim to improve motor function and aim to do so by reducing or eliminating the orthopedic problems that play a role in the impairment of function. Orthopedic problems can be found throughout the musculoskeletal system. The most common orthopedic deformities in the foot are equinus, pes planovalgus, clubfoot; knee flexion deformity in the knee, dysplasia in the hip and scoliosis in the spine. Management of equinus is often achieved with serial casts, botulinum toxin injection, and triceps surae muscle lengthening surgery. Pes planovalgus is treated with orthotic approaches and clubfoot is treated with serial casting and orthotics called ponseti method. In knee flexion deformity, ground reaction aphosis is considered to be a better treatment option than surgery. In hip dysplasia, the decision of conservative or surgical treatment is based on the migration percentage, which gives information about the amount of separation. The most current approach among conservative treatment options is hip abduction orthosis. Botulinum toxin injection accompanies the use of orthosis. Surgical options include open hip reduction, femoral varus derotation osteotomy, pelvic osteotomy or radical surgery. The treatment should be based on the clinical picture, the needs of the child with cerebral palsy and feedback from the family. Finally, scoliosis in cerebral palsy is mostly ’C’ shaped and in the thoracolumbar region. The use of a brace appropriate to the curvature is the first option. Surgery is preferred when vital functions are jeopardized and the most common surgical method is fusion surgery.
RUSSIA’S WAR: A EUROPEAN CHALLENGE FROM THE PERSPECTIVE OF A NEUTRAL STATE
Authors: Schneeberger Kati
Published: September 2024

The reactions in Europe, particularly in the EU member states, to Russia's full-scale invasion of Ukraine have been very diverse, including those of neutral countries. While Finland and Sweden joined NATO as quickly as possible, Austria continues to uphold its neutrality.
Russia is waging a hybrid war against all of Europe, which includes influencing the information space. As we now know, Germany was identified by Russia as an easy target and has been subjected to particularly intense manipulation, mainly due to its influence on decision-making at the EU level. The aim of these attacks is to pull states away from the Western alliance and turn them into advocates for Moscow. The political extremes, especially right-wing populists, could be strengthened by targeted propaganda and disinformation, leading to societal division and state destabilization.
Due to the shared language space, attacks on Germany also significantly affect public opinion in Austria. Additionally, Austria’s neutrality has become another target of Russian influence.
In addition to the much-discussed attacks on the internet, particularly on social media, there are other, less obvious channels of influence. These include paid or unpaid influencers who spread Russian positions as politicians or experts on television, in books, discussions, newspapers, etc. In Austria, Russian agents have even infiltrated the domestic intelligence service. This poses a significant threat to our security.
Improving the Privacy in Wireless-Enabled 5G Networks: A Lightweight Protocol for IIoT Communications
Authors: Saeed Mamoon , Saeed Rashid , Elbashier Mohammed , Ali Elmustafa , Mokhtar Rania , Ahmed Zeinab
Published: September 2024

The vision and major elements of the fifth generation (5G) ecosystem have previously been explored. We examine how security may impact the envisioned 5G wireless systems and the challenges and potential solutions to aid in these efforts and define the security and privacy aspects of 5G networks. 5G networks have provided solutions for quicker machine control, problem identification, performance analysis, and data access. Interaction between Internet of Things (IoT) nodes occur across an unsecured wireless channel, which has positive and negative effects. Despite being physically separated, unauthorized nodes could communicate via an unprotected wireless channel to gather data and take over industrial devices. Secure sessions can mitigate these risks, but it might be challenging to construct a secure session over a weak channel. To address this issue, the Variable Identification (VID) is used. VID offers a simple key exchange platform to authorized Industry Internet of Things (IIoT) nodes while guarding against unauthorized use. The lightweight changeable pseudonyms used by VID for trust-building are selected at random from a pool discovered in the home network and terminal devices. All IDs are chosen at random from a pool and are used to protect data against forgery, replay, alteration, impersonation, and man-in-the-middle attacks, among other things, between the home network and terminal equipment. The ProVerif tool is used to evaluate the suggested system, and the findings demonstrate that it is trustworthy and resistant to prospective attacks.
Improving Crowd Counting Performance: A Convolutional Neural Network Approach with Transfer Learning
Authors: Ahmeed Marwah , Khalifa Othman
Published: September 2024

Precise crowd counting is critical to public safety and smart city planning since it solves the problems associated with the time-consuming manual counting of people in photos and videos. Transfer learning has become a key building block for improving crowd counting techniques, especially when used to Convolutional Neural Networks (CNNs). Because pretrained models already know the pertinent weights and architecture, using them in transfer learning minimizes computational demands and shortens training time. This paper presents a crowd counting method with an emphasis on optimizing the VGG16 model with a mall dataset. The results show that using VGG16 for transfer learning leads to higher performance when compared to more modern methods like AdaCrowd and PSSW models. In addition, the paper highlights how adaptable our proposed method is and how well it can transfer knowledge from one dataset to another.
Do poder soberano à microfísica do poder: hábitos alimentares, convocações comunicacionais e produção de subjetividades
Authors: Teixeira Lucas de Vasconcelos
Published: September 2024

Partindo de uma pesquisa bibliográfica, propomos investigar as imbricações entre alimentação e poder, notadamente no que diz respeito aos hábitos alimentares, às ondas de midiatização e às relações de poder que perpassam a constituição dos sujeitos e os ordenamentos da vida desde o medievo até o contemporâneo. Buscamos respaldo na compreensão de que comer ultrapassa a função biológica e na microfísica do poder foucaultiano, isto é, uma prática social constituída historicamente por mecanismos e procedimentos que atuam no nível do social e que penetram na vida cotidiana dos sujeitos. Estas reflexões permitem observar os engendramentos das racionalidades senhoriais, mercantilistas e capitalistas nos ordenamentos da vida e na produção de subjetividades, bem como a emergência da comunicação midiática e seu protagonismo cada vez mais acentuado na sociedade de controle neoliberal.
O churrasco como emblema alimentar cultural, técnica e discussões sobre gênero
Authors: Corrêa Eduarda Merljak , Pozzatti Marianna
Published: September 2024

O universo do churrasco, para além de técnicas e cortes, possibilita reflexões sociais e culturais. Deste modo, abrange questões relacionadas ao papel da mulher nesse meio ainda predominantemente masculino. Assim, procurou-se analisar o funcionamento da prática do churrasco em São Borja-RS, Brasil, de modo a identificar a presença feminina nesses espaços. Para isso, foram realizadas entrevistas com questionário de perguntas abertas semiestruturadas com seis pessoas que já estão inseridas neste meio e a partir disso analisou-se e comparou-se as informações obtidas. Observou-se que, na cidade de São Borja, o gênero que ainda prevalece na execução do churrasco é o masculino, embora seja possível notar uma mudança neste cenário, a partir da introdução e naturalização da presença feminina neste meio. Para além disso, observa-se que o churrasco permeia um conjunto complexo de técnicas, ingredientes, cortes cárneos e aspectos culturais que enriquecem o assunto e tornam o ato de prepará-lo extremamente rico e subjetivo.
The Analysis Study of Diagnosis and Management of Respiratory Distress Syndrome in Neonatus: A Comprehensive Systematic Review
Authors: Aldila Raden Rara Farah , Erlich Willyam Arnando Deggas
Published: September 2024

Background: Respiratory distress syndrome (RDS) management is crucial for preterm infants, as it aims to improve survival rates while minimizing the risk of adverse outcomes, particularly bronchopulmonary dysplasia (BPD). Methods: This systematic review exclusively examined full-text articles published in English between 2014 and 2024, adhering to the PRISMA 2020 guidelines. Result: The study conducted a thorough review of more than 4000 publications that were obtained from reputable databases, such as ScienceDirect, SagePub, and PubMed. Eight publications were identified as necessitating a more comprehensive examination subsequent to an initial screening. As a result, a comprehensive review of these selected studies was conducted to guarantee an exhaustive and rigorous assessment. Conclusion: RDS diagnosis and management differ significantly between Japan and Europe, with neonatologists administering surfactants based on confirmed diagnosis rather than FiO2 levels. In Japan, chest radiography and stable microbubble rating are commonly used diagnostic tools, but accuracy can be influenced by variability and sample contamination. Challenges remain in distinguishing RDS from transient tachypnea.
Analisis Pengaruh Bopo, Ldr, Inflasi Dan Pertumbuhan Ekonomi Terhadap Return On Assets (Roa) Pada Bank Umum Milik Pemerintah Daerah Propinsi Di Indonesia
Authors: Sodikin Akhmad
Published: September 2024

Penelitian ini bertujuan untuk mengetahui pengaruh BOPO, LDR, Inflasi dan pertumbuhan ekonomi terhadap Return on Assets (ROA) baik secara simultan atau secara parsial. Penelitian dilakukan pada Perusahaan perbankan milik pemerintah propinsi di Indonesia. Data diambil dengan menggunakan metode saple purposive (purposive sampling) sebanyak 20 perusahaan. Data yang dikumpulkan dianalisis dengan menggunakan analisis regresi berganda dengan pengujian uji F untuk pengaruh simultan dan uji t untuk pengaruh parsial. Berdasarkan pada analisis data diketahui bahwa          nilai F pada regresi linear berganda sebesar 5,270 dan signifikansi sebesar 0,001 di atas nilai 0,05. Hal ini menunjukkan bahwa variabel BOPO, LDR, Inflasi dan pertumbuhan ekonomi berpengaruh terhadap ROA secara signifikan. Nilai koefisien untuk variabel BOPO sebesar -0,034. Nilai ini menunjukkan jika BOPO meningkat sebesar 1 % maka ROA menurun sebesar 0,034 % dengan asumsi variabel lainnya tetap. Pengaruh ini memiliki nilai t sebesar -3,645 dengan signifikansi sebesar 0,001. Nilai signifikansi ini lebih kecil dari 0,05 sehingga dapat dikatakan bahwa variabel BOPO berpengaruh signifikan terhadap ROA secara parsial.  Nilai koefisien LDR sebesar 0,032. Nilai ini menunjukkan bahwa jika LDR naik menjadi 1 % maka ROA akan meningkat juga sebesar 0,032 % dengan variabel lain dianggap tetap. Pengaruh ini memiliki nilai t sebesar 4,089 dan signifikansi 0,00 atau kurang dari 0,05. Hal ini menunjukkan bahwa variabel LDR berpengaruh terahdap ROA secara signifikan. Nilai koefisien inflasi adalah -0,024. Nilai ini menunjukkan bahwa jika inflasi naik 1 % maka ROA turun sebesar 0,024 % dengan asumsi variabel lain dianggap tetap. Nilai t pada koefisien ini sebesar -0,348 dan nilai signifikansi sebesar 0,729. Nilai signifikansi ini lebih besar dari 0,05. Hal ini menunjukkan bahwa variabel inflasi tidak berpengaruh terahadap ROA secara signifikan. Nilai koefisien pertumbuhan ekonomi sebesar -0,007. Nilai ini menunjukkan bahwa jika variabel pertumbuhan ekonomi naik sebesar 1 % % maka variabel ROA turun sebesar 0,007 %. Nilai t hitung sebesar -0,195 dengan signifikansi sebesar 0,846. Nilai signifikansi tersebut lebih besar dari 0,05. Hal ini berarti bahwa variabel pertumbuhan ekonomi tidak berpengaruh terhaap ROA secara signifikan.
Kajian Historis-Teologis Decade To Overcome Violence Oleh Dewan Gereja-Gereja Se-Dunia Dan Relevansinya Terhadap Teori Multikulturalisme Menurut John Rawls Dan Charles Taylor
Authors: MADARHAKAD  , STEPANUS
Published: September 2024

Pada dekade 2001-2010, Dewan Gereja-Gereja se-Dunia mencanangkan Decade to Overcome Violence (DOV) sebagai wujud nyata kerinduan sekaligus tanggungjawab gereja mewujudkan harapan dan kebutuhan masyarakat internasional membangun perdamaian dunia berdasarkan keadilan. Dalam hal ini gereja sebagai persekutuan orang-orang percaya kepada Yesus Kristus, terpanggil sekaligus bertanggungjawab mengambil bagian dalam upaya menghapuskan kekerasan demi mewujudkan perdamaian yang menyeluruh dan abadi. Sebagai bagian dari persekutuan orang percaya, setiap orang percaya juga terpanggil sekaligus bertanggungjawab menghapuskan kekerasan dan membangun perdamaian.
Penulis melihat adanya relevansi mengenai pemahaman Dewan Gereja-Gereja se-Dunia dengan dicanangkannya DOV tersebut,  dengan teori multikulturalisme yang digagas dua orang pemikir yakni  John Rawls dan Charles Taylor. Relevansi yang dimaksudkan dalam hal ini, terkait pentingnya peranan manusia sebagai individu sekaligus peranan manusia sebagai kolektifitas selaku mahluk sosial yang memang sangat dibutuhkan dalam upaya umat manusia membangun perdamaian berdasarkan keadilan. Menghapuskan kekerasaan dalam rangka membangun  perdamaian adalah harapan, dambaan dan kebutuhan setiap orang sekaligus kebutuhan bersama umat manusia kapan dan dimanapun.
Solidaritas Kesempurnaan Menurut Ibrani 11:35-40 : Sebuah Perspektif Kritis Terhadap Mati Syahid Sebelum Yesus
Authors: Jimmy Jimmy Sucipto , Aprinalia  , Friskilia Randa Prading  , Gunawan  , Jufri Susanto
Published: September 2024

Tujuan penulisan artikel ini adalah memberikan sumbangsi pemahaman terhadap paradigma mengenai mati syahid dalam kekristenan, dengan menonjolkan bagaimana ‘kesempurnaa dalam Kritus’ sebagai tolak ukur utama, melalui refleksi iman bagi orang-orang yang mati karena iman sebelum Kristus. Penjelasan ini akan terarah pada suatu konsep penyelesaian bahwa refleksi iman mereka lebih pada pemaknaan dan penyempurnaan itu. Metode penelisikan yang digunakan dalam jurnal ini adalah metode penelitian kualitatif deskriptif atau descriptive research yaitu metode atau pendekatan penelitian yang memberikan informasi sebanyak-banyaknya tentang objek penelitian, serta menjelaskan atau mendeskripsikan masalah atau keadaan dengan baik dengan angka maupun kata-kata. Penelitian ini mengaitkan berbagai sumber yakni sejumlah variabel yang berkenaan akan dibahas dengan berbagai sudut pandang yang berbeda mengikuti jumlah literatur yang ditemukan. Dari penelitian ditemukan bahwa bahwa salah satu sumbangsi teologis yaitu memaknai kesempurnaan dalam Kristus beserta merefleksikan iman adalah bahwa orang Kristen masa kini adalah subjek atau para perefleksi tersebut, dengan “solidaritas kesempurnaan” yaitu bagi mereka yang menunggu rencana indah dari Tuhan. Kematian dalam mempertahankan iman mereka adalah buktinya bahwa iman akan terus berkembang dengan pengalaman, sembari para pengikut Kristus menjadi agen “solidaritas kesempurnaan” bagi mereka.
A patologia da arte e da exclusão
Authors: Gadotti Claudia Morelli
Published: September 2024

Este trabalho propõe uma reflexão sobre os tênues limites que separam a patologia da criatividade, utilizando-se, para isso, da genialidade da escritora Clarice Lispector. Coloca a ideia da patologia como fenômeno associado à história da misoginia, buscando suas raízes arquetípicas no mito da criação e na figura feminina de Eva. Desenvolve também a relação entre essa misoginia no plano arquetípico e o sentimento de exclusão que vivenciamos nos mais diferentes níveis de relacionamentos.
Fracasso e morte no processo analítico
Authors: Oliveira Santina Rodrigues de
Published: September 2024

O presente artigo discorre sobre a constelação de um “complexo do fracasso” em sua interface com o tema arquetípico da morte no processo analítico. Por meio de um recorte de caso clínico, problematiza algumas dificuldades encontradas no manejo transferencial do processo psicoterapêutico de uma paciente diagnosticada como borderline, em especial os sentimentos contratransferenciais de frustração, abandono e raiva relacionados à impotência da analista diante do fim da análise. Tendo por referência textos de James Hillman e Rafael López-Pedraza, buscam-se elementos para discutir o estatuto da díade “fracasso-sucesso” nas bases epistemológicas da psicologia, de modo que reveja os limites dados a tais categorias no campo da psicoterapia e amplie os sentidos possíveis para as vivências de fracasso analítico.
Contribuições de animais na clínica: um estudo em psicologia analítica
Authors: Carvalho Heloísa Kuhnen Ferreira de , Guimarães Paula Pinheiro Varela , Catta-Preta Marisa Vicente
Published: September 2024

O presente trabalho busca compreender a presença de animais em consultório clínico como colaboradores do processo psicoterapêutico dos pacientes. O levantamento bibliográfico realizado indicou que os animais auxiliam o estabelecimento do vínculo entre paciente e psicólogo, especialmente no caso de crianças. A fim de atingir o objetivo proposto, realizaram-se entrevistas semidirigidas com cinco psicólogas que atendem em consultório particular com a presença de animais, de forma online ou presencial, sob a perspectiva da psicologia analítica. As entrevistas foram analisadas a partir desta abordagem e os resultados obtidos demonstraram que a presença de animais promove: alterações do espaço terapêutico, relação de vínculo entre paciente e terapeuta e criação de suporte emocional durante as sessões, tanto aos psicólogos quanto aos pacientes. Dessa forma, a pesquisa concluiu que animais podem ser ferramentas valiosas a serem utilizadas no setting terapêutico.
Da despedida para o viver na ausência: considerações sobre o luto e rituais de despedida a partir de vivências na pandemia
Authors: Zacarias Ludymilla , Amaral Natália Dantas do , Arrais Rebecca Holanda
Published: September 2024

Este artigo consiste em um estudo de três casos de luto vivenciados na pandemia de covid-19. Os dois primeiros são provenientes de pesquisa de mestrado, já o terceiro é um caso clínico de uma viúva em seu primeiro ano de luto. Identificamos e discutimos, conforme as perspectivas de Jung e de autores junguianos, que o fenômeno da morte desorganiza e constela o Caos. Contudo, rituais de despedida disseminados pela cultura e pelas religiões, ou mesmo criados pelo enlutado, podem auxiliar no processo de luto, possibilitando a criação de novos sentidos para a perda vivenciada. Constatamos que, quando não é possível a vivência destes rituais, pode haver risco e impacto na saúde mental dos enlutados.
Thinking Matter(s) in Theatre Practice
Authors: Eeg-Tverbakk Camilla
Published: September 2024

This article discusses a materialist approach to dramaturgy framed as a dramaturgy of assemblage. It is inspired by posthuman thinking and draws on theory from new materialism (Rosi Braidotti, Jane Bennett, Elaine Gan, and Anna Tsing). The dramaturgical approach is developed through artistic research, and the article refers to the performances Childism (2015) and Jeg vill høre havet (2017), which serve as examples of this practice. I articulate the movement from dramaturgy as a collective practice to exploring a collective which includes more-than-human collaborators. Rosi Bradotti’s work on the nomadic subject (drawing on Deleuze and Guattari) has inspired the notion of the nomadic dramaturge. In her book Posthuman Knowledge, Braidotti discusses what “we” are in the posthuman and post-anthropocentric condition, suggesting that the posthuman subject is (part of) a collective. Following Braidotti, I introduce the concept of dramaturgy of assemblages as a place for this collective subjectivity. A dramaturgy of assemblages responds in practice to the question of how a posthuman framework affects theatre and performance-making. What is presented in this article is all about shifting perspectives. When we think differently, we act differently, and different things are formed.
Comparative Study of Population-based Metaheuristic Algorithms in Case Study of DNA Sequence Assembly
Authors:   Department of Computer Science Education, Universitas Pendidikan Indonesia, Dr. Setiabudi Str. No. 229, Bandung 40154, Indonesia, Riza Lala Septem , Prasetyo Yudi ,   Department of Computer Science Education, Universitas Pendidikan Indonesia, Dr. Setiabudi Str. No. 229, Bandung 40154, Indonesia, Zain Muhammad Iqbal ,   Department of Computer Science Education, Universitas Pendidikan Indonesia, Dr. Setiabudi Str. No. 229, Bandung 40154, Indonesia, Siregar Herbert ,   Department of Computer Science Education, Universitas Pendidikan Indonesia, Dr. Setiabudi Str. No. 229, Bandung 40154, Indonesia, Megasari Rani ,   Department of Computer Science Education, Universitas Pendidikan Indonesia, Dr. Setiabudi Str. No. 229, Bandung 40154, Indonesia, Hidayat Topik ,   Department of Biology Education, Universitas Pendidikan Indonesia, Dr. Setiabudi Str. No. 229, Bandung 40154, Indonesia, Kusumawaty Diah ,   Department of Biology Education, Universitas Pendidikan Indonesia, Dr. Setiabudi Str. No. 229, Bandung 40154, Indonesia, Rosyda Miftahurrahma ,   Informatics Department, Universitas Ahmad Dahlan, Kapas 9 Str. Yogyakarta 55166, Indonesia
Published: September 2024

Modern technology encounters difficulties performing DNA sequencing on long DNA sequences. Therefore, longer DNA sequences must be cut into smaller fragments. DNA sequence assembly is the process of combining several short genome sequences to create a longer DNA sequence. This study aims to compare the performance of several population-based metaheuristic algorithms in handling the DNA sequence assembly problem based on computation time, number of contigs, and overlap value. The algorithms used in this study include the Honey Badger Algorithm (HBA), Lévy Flight Distribution (LFD), African Vultures Optimization Algorithm (AVOA), and Particle Swarm Optimization (PSO). Overall, AVOA has the best results where it can produce the most total overlap, where the most overlap is 49952 in the dataset with length 750 and coverage 25. AVOA also has the best efficiency because it has a faster computation time than other algorithms in all datasets. Besides AVOA, PSO produces total overlap and computation time that is not far from AVOA. However, based on the number of contigs, HBA is able to create the least number of contigs, especially on datasets with a length of 750 and coverage of 15, with a total of 6 contigs.
Evaluation of certain families of log-cosine integrals using hypergeometric function approach and applications
Authors:   Millia Islamia (A Central University), Qureshi Mohammad Idris , Malik Shakir Hussain ,   Department of Mathematics, Government Degree College
Published: September 2024

In this paper, we provide the analytical solutions of the families of certain definite integrals: $\int_0^\pi x^{m}\{\ln(2\cos\frac{x}{2})\}^{n}dx$ $(m\in\mathbb{N}_{0}$ and $n\in\mathbb{N}),$ in terms of multiple hypergeometric functions of Kampé de Fériet having the arguments $\pm1$ and Riemann zeta functions. As applications, we obtain some mixed summation formulas (19), (35) and (46) involving generalized hypergeometric functions $_3F_2,$ $_5F_4$ and $_7F_6$ having the arguments $\pm 1$ and other (possibly) new summation formulas (38) and (40) for multiple hypergeometric functions of Kampé de Fériet having the arguments $\pm 1$ also mixed relations (36) and (47) involving Riemann zeta functions.
Water and chloride as allosteric inhibitors in WNK kinase osmosensing
Authors: Teixeira Liliana R , Akella Radha , Humphreys John M , He Haixia , Goldsmith Elizabeth J
Published: September 2024

Osmotic stress and chloride regulate the autophosphorylation and activity of the WNK1 and WNK3 kinase domains. The kinase domain of unphosphorylated WNK1 (uWNK1) is an asymmetric dimer possessing water molecules conserved in multiple uWNK1 crystal structures. Conserved waters are present in two networks, referred to here as conserved water networks 1 and 2 (CWN1and CWN2). Here we show that PEG400 applied to crystals of dimeric uWNK1 induces de-dimerization. Both the WNK1 the water networks and the chloride binding site and are disrupted by PEG400. CWN1 is surrounded by a cluster of pan-WNK-conserved charged residues. Here we mutagenized these charges in WNK3, a highly active WNK isoform kinase domain, and WNK1, the isoform best studied crystallographically. Mutation of E314 in the Activation Loop of WNK3 (WNK3/E314Q and WNK3/E314A, and the homologous WNK1/E388A) enhanced the rate of autophosphorylation, and reduced chloride sensitivity. Other WNK3 Cluster mutants reduced the rate of autophosphorylation activity coupled with greater chloride sensitivity than wild-type. The water and chloride regulation thus appear linked. The lower activity of some mutants may reflect effects on catalysis. Crystallography showed that activating mutants introduced conformational changes in similar parts of the structure to those induced by PEG400. WNK activating mutations and crystallography support a role for CWN1 in WNK inhibition consistent with water functioning as an allosteric ligand.
Lost in translation: Inconvenient truths on the utility of mouse models in Alzheimer’s disease research
Authors: Granzotto Alberto , Vissel Bryce , Sensi Stefano L
Published: September 2024

The recent, controversial approval of antibody-based treatments for Alzheimer’s disease (AD) is fueling a heated debate on the molecular determinants of this condition. The discussion should also incorporate a critical revision of the limitations of preclinical mouse models in advancing our understanding of AD. We critically discuss the limitations of animal models, stressing the need for careful consideration of how experiments are designed and results interpreted. We identify the shortcomings of AD models to recapitulate the complexity of the human disease. We dissect these issues at the quantitative, qualitative, temporal, and context-dependent levels. We argue that these models are based on the oversimplistic assumptions proposed by the amyloid cascade hypothesis (ACH) of AD and fail to account for the multifactorial nature of the condition. By shedding light on the constraints of current experimental tools, this review aims to foster the development and implementation of more clinically relevant tools. While we do not rule out a role for preclinical models, we call for alternative approaches to be explored and, most importantly, for a re-evaluation of the ACH.
A conserved cell-pole determinant organizes proper polar flagellum formation
Authors: Arroyo-Pérez Erick Eligio , Hook John C , Alvarado Alejandra , Wimmi Stephan , Glatter Timo , Thormann Kai M , Ringgaard Simon
Published: September 2024

The coordination of cell cycle progression and flagellar synthesis is a complex process in motile bacteria. In γ-proteobacteria, the localization of the flagellum to the cell pole is mediated by the SRP-type GTPase FlhF. However, the mechanism of action of FlhF, and its relationship with the cell pole landmark protein HubP remain unclear. In this study, we discovered a novel protein called FipA that is required for normal FlhF activity and function in polar flagellar synthesis. We demonstrated that membrane-localized FipA interacts with FlhF and is required for normal flagellar synthesis in
Vibrio parahaemolyticus
,
Pseudomonas putida
, and
Shewanella putrefaciens
, and it does so independently of the polar localization mediated by HubP. FipA exhibits a dynamic localization pattern and is present at the designated pole before flagellar synthesis begins, suggesting its role in licensing flagellar formation. This discovery provides insight into a new pathway for regulating flagellum synthesis and coordinating cellular organization in bacteria that rely on polar flagellation and FlhF-dependent localization.
The intrinsically disordered N-terminus of SUMO1 is an intramolecular inhibitor of SUMO1 interactions
Authors: Richter Sebastian M , Jin Fan , Ritterhoff Tobias , Fergin Aleksandra , Maurer Eric , Frank Andrea , Daube Michael , Hajnal Alex , Klevit Rachel , Gräter Frauke , Flotho Annette , Melchior Frauke
Published: September 2024

Ubiquitin-related proteins of the SUMO family are reversibly attached to thousands of proteins in eukaryotic cells. Many SUMO substrates, effectors and enzymes carry short motifs (SIMs) that mediate low affinity interactions with SUMO proteins. How specificity is achieved in target selection, SUMO paralogue choice and SUMO-dependent interactions is largely unknown. A unique but poorly understood feature of SUMO proteins is their intrinsically disordered N-terminus. We reveal a function for N-termini of human,
C. elegans,
and yeast SUMO proteins as intramolecular inhibitors of SUMO- SIM interactions. Mutational analyses, NMR spectroscopy, and Molecular Dynamics simulations indicate that SUMO’s N-terminus can inhibit SIM binding by fast and fuzzy interactions with SUMO‘s core. Deletion of the
C. elegans
SUMO1 N-terminus leads to p53-dependent apoptosis during germline development, indicating an important role in DNA damage repair. Our findings reveal a mechanism of disorder-based autoinhibition that contributes to the specificity of SUMOylation and SUMO-dependent interactions.
Human induced pluripotent stem cell-derived cardiomyocytes to study inflammation-induced aberrant calcium transient
Authors: Tatekoshi Yuki , Chen Chunlei , Shapiro Jason Solomon , Chang Hsiang-Chun , Blancard Malorie , Lyra-Leite Davi M , Burridge Paul W , Feinstein Matthew , D'Aquila Richard , Hsue Priscilla , Ardehali Hossein
Published: September 2024

Heart failure with preserved ejection fraction (HFpEF) is commonly found in persons living with HIV (PLWH) even when antiretroviral therapy suppresses HIV viremia. However, studying this condition has been challenging because an appropriate animal model is not available. In this article, we studied calcium transient in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in culture to simulate the cardiomyocyte relaxation defect noted in PLWH and HFpEF and assess whether various drugs have an effect. We show that treatment of hiPSC-CMs with inflammatory cytokines (such as interferon-γ or TNF-α) impairs their Ca2+ uptake into sarcoplasmic reticulum and that SGLT2 inhibitors, clinically proven as effective for HFpEF, reverse this effect. Additionally, treatment with mitochondrial antioxidants (like mito-Tempo) and certain antiretrovirals resulted in the reversal of the effects of these cytokines on calcium transient. Finally, incubation of hiPSC-CMs with serum from HIV patients with and without diastolic dysfunction did not alter their Ca2+-decay time, indicating that the exposure to the serum of these patients is not sufficient to induce the decrease in Ca2+ uptake in vitro. Together, our results indicate that hiPSC-CMs can be used as a model to study molecular mechanisms of inflammation-mediated abnormal cardiomyocyte relaxation and screen for potential new interventions.
Merging multi-omics with proteome integral solubility alteration unveils antibiotic mode of action
Authors: Maity Ritwik , Zhang Xuepei , Liberati Francesca Romana , Scribani Rossi Chiara , Cutruzzolá Francesca , Rinaldo Serena , Gaetani Massimiliano , Aínsa José Antonio , Sancho Javier
Published: September 2024

Antimicrobial resistance is responsible for an alarming number of deaths, estimated at 5 million per year. To combat priority pathogens, like Helicobacter pylori, the development of novel therapies is of utmost importance. Understanding the molecular alterations induced by medications is critical for the design of multi-targeting treatments capable of eradicating the infection and mitigating its pathogenicity. However, the application of bulk omics approaches for unraveling drug molecular mechanisms of action is limited by their inability to discriminate between target-specific modifications and off-target effects. This study introduces a multi-omics method to overcome the existing limitation. For the first time, the Proteome Integral Solubility Alteration (PISA) assay is utilized in bacteria in the PISA-Express format to link proteome solubility with different and potentially immediate responses to drug treatment, enabling us the resolution to understand target-specific modifications and off-target effects. This study introduces a comprehensive method for understanding drug mechanisms and optimizing the development of multi-targeting antimicrobial therapies.
Task-dependent coarticulation of movement sequences
Authors: Kalidindi Hari Teja , Crevecoeur Frederic
Published: September 2024

Combining individual actions into sequences is a hallmark of everyday activities. Classical theories propose that the motor system forms a single specification of the sequence as a whole, leading to the coarticulation of the different elements. In contrast, recent neural recordings challenge this idea and suggest independent execution of each element specified separately. Here, we show that separate or coarticulated sequences can result from the same task-dependent controller, without implying different representations in the brain. Simulations show that planning for multiple reaches simultaneously allows separate or coarticulated sequences depending on instructions about intermediate goals. Human experiments in a two-reach sequence task validated this model. Furthermore, in co-articulated sequences, the second goal influenced long-latency stretch responses to external loads applied during the first reach, demonstrating the involvement of the sensorimotor network supporting fast feedback control. Overall, our study establishes a computational framework for sequence production that highlights the importance of feedback control in this essential motor skill.
High-frequency terahertz stimulation alleviates neuropathic pain by inhibiting the pyramidal neuron activity in the anterior cingulate cortex of mice
Authors: Peng Wenyu , Wang Pan , Tan Chaoyang , Zhao Han , Chen Kun , Si Huaxing , Tian Yuchen , Lou Anxin , Zhu Zhi , Yuan Yifang , Wu Kaijie , Chang Chao , Wu Yuanming , Chen Tao
Published: September 2024

Neuropathic pain (NP) is caused by a lesion or disease of the somatosensory system and is characterized by abnormal hypersensitivity to stimuli and nociceptive responses to non-noxious stimuli, affecting approximately 7–10% of the general population. However, current first-line drugs like non-steroidal anti-inflammatory agents and opioids have limitations, including dose-limiting side effects, dependence, and tolerability issues. Therefore, developing new interventions for the management of NP is urgent. In this study, we discovered that the high-frequency terahertz stimulation (HFTS) at approximately 36 THz effectively alleviates NP symptoms in mice with spared nerve injury. Computational simulation suggests that the frequency resonates with the carbonyl group in the filter region of Kv1.2 channels, facilitating the translocation of potassium ions. In vivo and in vitro results demonstrate that HFTS reduces the excitability of pyramidal neurons in the anterior cingulate cortex likely through enhancing the voltage-gated K+ and also the leak K+ conductance. This research presents a novel optical intervention strategy with terahertz waves for the treatment of NP and holds promising applications in other nervous system diseases.
Reproducible, data-driven characterization of sleep based on brain dynamics and transitions from whole-night fMRI
Authors: Yang Fan Nils , Picchioni Dante , de Zwart Jacco A , Wang Yicun , van Gelderen Peter , Duyn Jeff H
Published: September 2024

Understanding the function of sleep requires studying the dynamics of brain activity across whole-night sleep and their transitions. However, current gold standard polysomnography (PSG) has limited spatial resolution to track brain activity. Additionally, previous fMRI studies were too short to capture full sleep stages and their cycling. To study whole-brain dynamics and transitions across whole-night sleep, we used an unsupervised learning approach, the Hidden Markov model (HMM), on two-night, 16 hr fMRI recordings of 12 non-sleep-deprived participants who reached all PSG-based sleep stages. This method identified 21 recurring brain states and their transition probabilities, beyond PSG-defined sleep stages. The HMM trained on one night accurately predicted the other, demonstrating unprecedented reproducibility. We also found functionally relevant subdivisions within rapid eye movement (REM) and within non-REM 2 stages. This study provides new insights into brain dynamics and transitions during sleep, aiding our understanding of sleep disorders that impact sleep transitions.