bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of the Addition of Humic Substances as Growth Promoter in Broiler Chickens Under Two Feeding Regimens
Authors: Domínguez-Negrete Alejandra , Gómez-Rosales Sergio , Angeles María de Lourdes , López-Hernández Luis Humberto , Reis-de Souza Tercia Cesaria , López-García Yair , Zavala-Franco Anai , Téllez-Isaias Guillermo
Published: December 2019

Humic substances (HS) from different sources have been evaluated to replace or reduce the use of growth promoter antibiotics (GPA) in the feeds of broiler chickens. The objective was to evaluate the growth performance, tibia measurements, nutrient balance, meat quality, and microbiological status of broiler fed with an HS extract (EHS) under ad libitum (ADLIB) or feed restriction (REST). Individually caged broilers (n = 180, 14–35 day of age) were assigned to a factorial arrangement of three dietary treatments: (1) positive control with bacitracin methylene disalicylate (BMD) and salinomycin; (2) negative control without BMD nor salinomycin, and (3) same as negative control with 0.25% EHS, and two feeding regimens 1) ADLIB or REST for 24 h on d 1, 7, and 14. Results were subjected to ANOVA. Positive control and EHS-fed broilers showed higher carcass yield (p < 0.05) and lower oocyst excretion (p < 0.01) compared to negative control birds. Lactic acid bacteria (LAB) and Clostridium perfringens (C. perfringens) were higher in negative control and EHS-broilers compared to positive control (p < 0.01). In conclusion, higher carcass yield, lower C. perfringens and oocyst excretion were found in positive control and higher carcass yield, higher LAB and lower oocyst excretion were found in EHS-fed broilers. Broilers subjected to REST had reduced growth performance and meat quality. In conclusion, EHS could be used to increase the carcass yield and beneficial LAB in broilers.
Applying the 3Rs: A Case Study on Evidence and Perceptions Relating to Rat Cage Height in the UK
Authors: Mazhary Hibba , Hawkins Penny
Published: December 2019

This article investigates the barriers to implementing higher caging in animal research establishments in the UK. The use of animals in research and testing in the UK is regulated by the Animals (Scientific Procedures) Act 1986, which sets out how animal experiments must be licensed and regulated. Within this, the Code of Practice currently allows laboratory rats to be housed in cages that are 20 cm high, even though adults can rear up to 30 cm. Most adult rats therefore cannot stand upright in ‘standard’ cages. We found that the main factors hindering the implementation of higher caging were classified into five different groups; health and safety, financial, animal welfare, scientific, and ‘human’. Suggestions to overcome these barriers are provided, as well as alternative animal welfare changes that can be put into place. We conclude that much of the desired evidence for moving to higher cages is already available, and therefore the focus should be on education and improving access to the existing evidence, in order to encourage facilities to work around existing financial and health and safety concerns.
Achievement of Accurate Robotic Arm-based Bike Frame Quality Check Using 3D Geometry Mathematical Model
Authors: Lin Hsiung-Cheng , Yu Bo-Ren , Wang Jen-Yu , Lai Jun-Ze , Wu Jia-Yang
Published: December 2019

Currently, the bike frame quality check (QC) mostly relies on human operation in industry. However, some drawbacks such as it being time-consuming, having low accuracy and involving non-computerized processes are still unavoidable. Apart from these problems,measured data are difficult to systematically analyze for tracking sources of product defects in the production process. For this reason, this paper aims to develop a 3D geometry mathematical model suitable for bicycle frames QC using robotic arm-based measurement. Unlike the traditional way to find coefficients of a space sphere, the proposed model requires only three check point coordinates to achieve the sphere axis coordinate and its radius. In the practical work, the contact sensor combined with the robotic arm is used to realize the compliance items measurement in shaft length, internal diameter, verticality, parallelism, etc. The proposed model is validated based on both mathematic verification and actual bike frame check.
OpenBIM: An Enabling Solution for Information Interoperability
Authors: Jiang Shaohua , Jiang Liping , Han Yunwei , Wu Zheng , Wang Na
Published: December 2019

The expansion of scale and the increase of complexity of construction projects puts higher requirements on the level of collaboration among different stakeholders. How to realize better information interoperability among multiple disciplines and different software platforms becomes a key problem in the collaborative process. openBIM (building information model), as a common approach of information exchange, can meet the needs of information interaction among different software well and improve the efficiency and accuracy of collaboration. To the best of our knowledge, there is currently no comprehensive survey of openBIM approach in the context of the AEC (Architecture, Engineering & Construction) industry, this paper fills the gap and presents a literature review of openBIM. In this paper, the openBIM related standards, software platforms, and tools enabling information interoperability are introduced and analyzed comprehensively based on related websites and literature. Furthermore, engineering information interoperability research supported by openBIM is analyzed from the perspectives of information representation, information query, information exchange, information extension, and information integration. Finally, research gaps and future directions are presented based on the analysis of existing research. The systematic analysis of the theory and practice of openBIM in this paper can provide support for its further research and application.
Using Real-Time Dynamic Prediction to Implement IoV-Based Collision Avoidance
Authors: Chang Che-Cheng , Lin Wei-Ming , Lai Chuan-An
Published: December 2019

For some IoV-based collision-avoidance architectures, it is not necessary that all vehicles have communication abilities. Hence, they need some particular designs and extra components. In the literature, one of them uses a camera mounted onto the infrastructure at an intersection to realize collision detection. Consequently, technologies for real-time object detection and dynamic prediction are required for the purposes of collision avoidance. In this paper, we propose an interesting method to predict the future position of a vehicle based on a well-known, real-time object detection project, YOLOv3. Our algorithm utilizes the concept of vehicle dynamics and the confidence region to predict the future position on vehicles. This will help us to realize real-time dynamic prediction and Internet of Vehicles (IoV)-based collision detection. Lastly, in accordance with the experimental results, our design shows the performance for predicting the future position of a vehicle.
An Analytical Framework in OFDM Wireless Networks Servicing Random or Quasi-Random Traffic
Authors: Panagoulias Panagiotis I. , Moscholios Ioannis D. , Sarigiannidis Panagiotis G. , Głąbowski Mariusz , Logothetis Michael D.
Published: December 2019

We consider the downlink of an orthogonal frequency division multiplexing (OFDM)-based cell that services calls from many service-classes. The call arrival process is random (Poisson) or quasi-random, i.e., calls are generated by an infinite or a finite number of sources, respectively. In order to determine congestion probabilities and resource utilization, we model the cell as a multirate loss model. Regarding the call admission, we consider the restricted accessibility, the bandwidth reservation (BR), and the complete sharing (CS) policies. In a system of restricted accessibility, a new call may be blocked even if resources do exist. In a BR system, subcarriers can be reserved in favor of calls of high subcarrier requirements. Finally, in a CS system, a new call is blocked due to resource unavailability. In all three policies, we show that there exist recursive formulas for the determination of the various performance measures. Based on simulation, the accuracy of the proposed formulas is found to be quite satisfactory.
Solvent Effects in Highly Efficient Light-Induced Molecular Aggregation
Authors: Shirakawa Masayuki , Kobayashi Takayoshi , Tokunaga Eiji
Published: December 2019

It has been reported that when irradiated with laser light non-resonant with the main absorption peaks, porphyrin molecules (4-[10,15,20-tris(4-sulfophenyl)-21,24-dihydroporphyrin-5-yl]benzenesulfonic acid, TPPS) in an aqueous solution become 10,000 to 100,000 times more efficient in light-induced molecular aggregation than expected from the ratio of gradient force potential to the thermal energy of molecules at room temperature. To determine the mechanism of this phenomenon, experiments on the light-induced aggregation of TPPS in alcohol solutions (methanol, ethanol, and butanol) were performed. In these alcohol solutions, the absorbance change was orders of magnitude smaller than in the aqueous solution. Furthermore, it was found that the absorbance change in the aqueous solution tended to be saturated with the increase of the irradiation intensity, but in the ethanol solution, the absorbance change increased linearly. These results can be qualitatively explained by the model in which intermolecular light-induced interactions between molecules within a close distance among randomly distributed molecules in the laser irradiation volume are highly relevant to the signal intensity. However, conventional dipole–dipole interactions, such as the Keesom interaction, are not quantitatively consistent with the results.
A Supercritical CO2 Waste Heat Recovery System Design for a Diesel Generator for Nuclear Power Plant Application
Authors: Ham Jin , Kim Min , Oh Bong , Son Seongmin , Lee Jekyoung , Lee Jeong
Published: December 2019

After the Fukushima accident, the importance of an emergency power supply for a nuclear power plant has been emphasized more. In order to maximize the performance of the existing emergency power source in operating nuclear power plants, adding a waste heat recovery system for the emergency power source is suggested for the first time in this study. In order to explore the possibility of the idea, a comparison of six supercritical carbon dioxide (S-CO2) power cycle layouts recovering waste heat from a 7.2 MW alternate alternating current diesel generator (AAC DG) is first presented. The diesel engine can supply two heat sources to the waste heat recovery system: one from exhaust gas and the other from scavenged air. Moreover, a sensitivity study of the cycles for different design parameters is performed, and the thermodynamic performances of the various cycles were evaluated. The main components, including turbomachinery and heat exchangers, are designed with in-house codes which have been validated with experiment data. Based on the designed cycle and components, the bottoming S-CO2 cycle performance under part load operating condition of AAC DG is analyzed by using a quasi-steady state cycle analysis method. It was found that a partial heating cycle has relatively higher net produced work while enjoying the benefit of a simple layout and smaller number of components. This study also revealed that further waste heat can be recovered by adjusting the flow split merging point of the partial heating cycle.
An Analytical Framework for the Investigation of Tropical Cyclone Wind Characteristics over Different Measurement Conditions
Authors: Li Lixiao , Zhou Yizhuo , Wang Haifeng , Zhou Haijun , He Xuhui , Wu Teng
Published: December 2019

Wind characteristics (e.g., mean wind speed, gust factor, turbulence intensity and integral scale, etc.) are quite scattered in different measurement conditions, especially during typhoon and/or hurricane processes, which results in the structural engineer ambiguously determining the wind parameters in wind-resistant design of buildings and structures in cyclone-prone regions. In tropical cyclones (including typhoons and hurricanes), the inconsistent wind characteristics may be in part ascribed to the complex flow structure with the coexistence of both mechanical and convective turbulence in the boundary layer of tropical cyclones. Another significant contribution to the scattered wind characteristics is due to various measurement conditions (e.g., terrain exposure and height) and data processing schemes (e.g., averaging time). The removal of the inconsistency in the field-measurement system may offer a more rational comparison of measured wind data from various observation platforms, and hence facilitates a better identification scheme of the wind characteristics to guide the urban planning design and wind-resistant design of buildings and structures. In this study, an analytical framework was firstly proposed to eliminate the potential observation-related effects in wind characteristics and then the wind characteristics of seven field measured tropical cyclones (four typhoons and three hurricanes) were comparatively investigated. Specifically, field measurements of wind characteristics were converted to a standard reference station with a roughness length of 0.03 m, observation duration of 10 min for mean wind and averaging time of 3 s for gusty wind at a 10 m height. The differences of the measured wind characteristics between the typhoons and hurricanes were highlighted. The standardized turbulent wind characteristics under the analytical framework for typhoons and hurricanes were compared with the corresponding recommendations in standard of American Society of Civil Engineers (ASCE 7-10) and Architectural Institute of Japan Recommendations for Loads on Buildings (AIJ-RLB-2004).
Influence of the Anthropogenic Fugitive, Combustion, and Industrial Dust on Winter Air Quality in East Asia
Authors: Jeong Jaein I. , Park Rokjin J.
Published: December 2019

We estimate the effects of the anthropogenic fugitive, combustion, and industrial dust (AFCID) on winter air quality in China and South Korea for November 2015–March 2016 using the Comprehensive Regional Emissions inventory for Atmospheric Transport Experiment (KU-CREATE) monthly anthropogenic emission inventory in conjunction with a nested version of GEOS-Chem. Including AFCID emissions in models results in a better agreement with observations and a reduced normalized mean bias of −28% compared to −40% without AFCID. Furthermore, we find that AFCID amounts to winter PM10 concentrations of 17.9 μg m−3 (17%) in eastern China (30−40° N, 112−120° E) with the largest contribution of AFCID to winter PM10 concentrations of up to 45 μg m−3 occurring in eastern China causing a significant impact on air quality to downwind regions. Including AFCID in the model results in an increase of simulated winter PM10 concentrations in South Korea by 3.1 μg m−3 (9%), of which transboundary transport from China accounts for more than 70% of this increased PM10 concentration. Our results indicate that AFCID is an essential factor for winter PM10 concentrations over East Asia and its sources and physical characteristics need to be better quantified to improve PM air quality forecasts.
Aqueous Reactions of Sulfate Radical-Anions with Nitrophenols in Atmospheric Context
Authors: Rudziński Krzysztof J. , Szmigielski Rafał
Published: December 2019

Nitrophenols, hazardous environmental pollutants, react promptly with atmospheric oxidants such as hydroxyl or nitrate radicals. This work aimed to estimate how fast nitrophenols are removed from the atmosphere by the aqueous-phase reactions with sulfate radical-anions. The reversed-rates method was applied to determine the relative rate constants for reactions of 2-nitrophenol, 3-nitrophenol, 4-nitrophenol, 2,4-dinitrophenol, and 2,4,6-trinitrophenol with sulfate radical-anions generated by the autoxidation of sodium sulfite catalyzed by iron(III) cations at ~298 K. The constants determined were: 9.08 × 108, 1.72 × 109, 6.60 × 108, 2.86 × 108, and 7.10 × 107 M−1 s−1, respectively. These values correlated linearly with the sums of Brown substituent coefficients and with the relative strength of the O–H bond of the respective nitrophenols. Rough estimation showed that the gas-phase reactions of 2-nitrophenol with hydroxyl or nitrate radicals dominated over the aqueous-phase reaction with sulfate radical-anions in deliquescent aerosol and haze water. In clouds, rains, and haze water, the aqueous-phase reaction of 2-nitrophenol with sulfate radical-anions dominated, provided the concentration of the radical-anions was not smaller than that of the hydroxyl or nitrate radicals. The results presented may be also interesting for designers of advanced oxidation processes for the removal of nitrophenol.
Brassinosteroids Induce Strong, Dose-Dependent Inhibition of Etiolated Pea Seedling Growth Correlated with Ethylene Production
Authors: Jiroutová Petra , Mikulík Jaromír , Novák Ondřej , Strnad Miroslav , Oklestkova Jana
Published: December 2019

We have recently discovered that brassinosteroids (BRs) can inhibit the growth of etiolated pea seedlings dose-dependently in a similar manner to the ‘triple response’ induced by ethylene. We demonstrate here that the growth inhibition of etiolated pea shoots strongly correlates with increases in ethylene production, which also responds dose-dependently to applied BRs. We assessed the biological activities of two natural BRs on pea seedlings, which are excellent material as they grow rapidly, and respond both linearly and uni-phasically to applied BRs. We then compared the BRs’ inhibitory effects on growth, and induction of ethylene and ACC (1-aminocyclopropane-1-carboxylic acid) production, to those of representatives of other phytohormone classes (cytokinins, auxins, and gibberellins). Auxin induced ca. 50-fold weaker responses in etiolated pea seedlings than brassinolide, and the other phytohormones induced much weaker (or opposite) responses. Following the optimization of conditions for determining ethylene production after BR treatment, we found a positive correlation between BR bioactivity and ethylene production. Finally, we optimized conditions for pea growth responses and developed a new, highly sensitive, and convenient bioassay for BR activity.
Role of MyD88 in IL-1β and Ethanol Modulation of GABAergic Transmission in the Central Amygdala
Authors: Bajo Michal , Patel Reesha R. , Hedges David M. , Varodayan Florence P. , Vlkolinsky Roman , Davis Tony D. , Burkart Michael D. , Blednov Yuri A. , Roberto Marisa
Published: December 2019

Myeloid differentiation primary response protein (MyD88) is a critical neuroimmune adaptor protein in TLR (Toll-like receptor) and IL-1R (Interleukin-1 receptor) signaling complexes. These two pro-inflammatory families play an important role in the neurobiology of alcohol use disorder, specifically MyD88 regulates ethanol drinking, ethanol-induced sedation, and ethanol-induced deficits in motor coordination. In this study, we examined the role of MyD88 in mediating the effects of IL-1β and ethanol on GABAergic transmission in the central amygdala (CeA) of male mice using whole-cell patch-clamp recordings in combination with pharmacological (AS-1, a mimetic that prevents MyD88 recruitment by IL-1R) and genetic (Myd88 knockout mice) approaches. We demonstrate through both approaches that IL-1β and ethanol’s modulatory effects at CeA GABA synapses are not dependent on MyD88. Myd88 knockout potentiated IL-1β’s actions in reducing postsynaptic GABAA receptor function. Pharmacological inhibition of MyD88 modulates IL-1β’s action at CeA GABA synapses similar to Myd88 knockout mice. Additionally, ethanol-induced CeA GABA release was greater in Myd88 knockout mice compared to wildtype controls. Thus, MyD88 is not essential to IL-1β or ethanol regulation of CeA GABA synapses but plays a role in modulating the magnitude of their effects, which may be a potential mechanism by which it regulates ethanol-related behaviors.
The Bulgarian Version of the Multidimensional Driving Style Inventory: Psychometric Properties
Authors: Totkova Zornitsa , Racheva Rositsa
Published: December 2019

Road safety is one of the main priorities for the European Union. Different strategies and policies strive to increase the level of road safety across Europe and although this level has increased in the last couple of years the number of injuries and fatalities resulting from traffic accidents is still very high. The multidimensional driving style inventory (MDSI) is a self-reported instrument for the assessment of a person’s habitual driving style and its connection to risky driving behaviour and involvement in different traffic accidents. The instrument was originally developed in Israel and there are several previous adaptations in different countries such as Argentina and Romania. The main objective of this study is to develop a valid and reliable version of the MDSI in Bulgaria. A study was conducted to evaluate the construction validity of the instrument and to test the validity of the factors in a Bulgarian sample (n = 456, male = 204; female = 252; average age = 37). Eight factors representing a specific driving style—dissociative, anxious, risky, angry, high-velocity, distress reduction, patient and careful and irrational—identified by previous versions of the MDSI are included in this study. The overall number of items in the inventory is 57 with Cronbach’s alpha = 0.78. The current study in Bulgaria confirmed the structural organization of the initial version of the inventory. The results of the conducted study supported the reliability and validity of the Bulgarian version of the MDSI. The possible implementation of the instrument for the development of different programs for preventions and interventions is discussed here within.
Security or Safety: Quantitative and Comparative Analysis of Usage in Research Works Published in 2004–2019
Authors: Bubnovskaia Olesia V. , Leonidova Vitalina V. , Lysova Alexandra V.
Published: December 2019

This article is devoted to the statistical analysis of security and safety frequency in the context of categories connected with social institutions and personality features in research works from 2004–2019. Research was based on the following methods: quantitative analysis of safety frequency in the context with coded “categories” related to social institutions and personality features; analysis was conducted with computer-assisted content analysis QDA Miner Lite v. 1.4 and Fisher’s F-test. An analysis of 1157 works showed that the terms “security” and “safety” were quantitatively more frequent when used with concepts related to social institutions than with concepts related to personality features. In our opinion, this qualitative trend shows the prevailing significance of social aspects of security over its personal (psychological) traits for research analysis and practical social aspects. The priority usage of the terms “security” and “safety” can be related to the securitization of society, (i.e., to the increased role and significance of social ways of providing security and protection from threats), primarily with the help of external law-enforcing actors such as the state, police, and army. Securitization counterweights the development of social and psychological mechanisms of security—developing motivation for safe behavior, personal self-regulation, and self-production of security as an internal feeling of protection.
Poly(ADP-Ribose) Polymerase Inhibitors in Pancreatic Cancer: A New Treatment Paradigms and Future Implications
Authors: Gupta Medhavi , Iyer Renuka , Fountzilas Christos
Published: December 2019

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy. Most of the patients of PDAC present at later stages of disease and have a five-year survival rate of less than 10%. About 5–10% PDAC cases are hereditary in nature and have DNA damage repair (DDR) mutations such as BRCA 1 and 2. Besides having implications on screening and prevention strategies, these mutations can confer sensitivity to platinum-based therapies and determine eligibility for poly(ADP-ribose) polymerase inhibitors (PARPi). In the presence of DDR mutations and PARPi, the cells are unable to utilize the error-free process of homologous recombination repair, leading to accumulation of double stranded DNA breaks and cell death eventually. Various PARPi are in clinical development in PDAC in different subgroup of patients as monotherapies and in combination with other therapeutics. This review would focus on the mechanism of action of PARPi, history of development in PDAC, resistance mechanisms and future directions.
Chromium Oxide Supported on Silicalite-1 Zeolite as a Novel Efficient Catalyst for Dehydrogenation of Isobutane Assisted by CO2
Authors: Luo Yajun , Miao Changxi , Yue Yinghong , Yang Weimin , Hua Weiming , Gao Zi
Published: December 2019

The chromium oxide catalysts supported on silicalite-1 zeolite (Cr/S-1) with a Cr content between 0.5% and 7% were synthesized via an incipient wetness method. The catalysts were characterized by XRD, N2 adsorption, TEM-EDX, UV-vis, DRIFTS, 29Si MAS NMR, XPS, H2-TPR, and NH3-TPD. The optimum 3%Cr/S-1 catalyst with 3%Cr is more active and stable than SBA-15-supported one with the same Cr content, which is a consequence of a higher content of Cr6+ in the fresh 3%Cr/S-1 catalyst and a higher content of Cr6+ retained on the former catalyst during the reaction. The 3%Cr/S-1 catalyst affords an isobutane conversion of 36.5% with 71.2% isobutene selectivity. The catalytic activity is well correlated with the content of Cr6+ in the fresh catalysts. Carbon dioxide displays a promoting effect on the dehydrogenation reaction.
Titanium and Vanadium Catalysts with 2-Hydroxyphenyloxazoline and Oxazine Ligands for Ethylene-Norbornene (co)Polymerization
Authors: Ochędzan-Siodłak Wioletta , Siodłak Dawid , Piontek Aleksandra , Doležal Karel
Published: December 2019

A series of titanium and vanadium complexes with oxazoline 2-(4,5-dihydro-1,3-oxazol-2-yl)phenol (L1), 2-(4-methyl-4,5-dihydro-1,3-oxazol-2-yl)phenol (L2), and oxazine 2-(5,6-dihydro-4H-1,3-oxazin-2-yl)phenol (L3) ligands were synthesized, and their structures were determined by NMR and MS methods as (L)2MtCl2. The vanadium complexes were found to be highly active in ethylene (7300 kgPE/(molV·h)) and ethylene/norbornene (5300 kgCop/(molV·h)) (co)polymerization. The polyethylene characteristics were melting temperature (123–142 °C), crystallinity degree (49–75%), molecular weight (5.7–8.5 × 105 g/mol), molecular weight distribution (1.5–2.4). The ethylene-norbornene (E-NB) copolymer characteristics were molecular weight (2.6–0.9 × 105 g/mol), molecular weight distribution (1.6–2.2), glass transition temperature (4–62 °C), norbornene incorporation (12.3–30.1 mol%) at initial concentration (0.5–1.5 mol/L). The microstructure of E-NB copolymers depends on the catalyst applied with the highest diads content for the (L3)2VCl2 and triads for the (L2)2VCl2 complexes.
Preparation and Analysis of Ni–Co Catalyst Use for Electricity Production and COD Reduction in Microbial Fuel Cells
Authors: Włodarczyk Paweł P. , Włodarczyk Barbara
Published: December 2019

Microbial fuel cells (MFCs) are devices than can contribute to the development of new technologies using renewable energy sources or waste products for energy production. Moreover, MFCs can realize wastewater pre-treatment, e.g., reduction of the chemical oxygen demand (COD). This research covered preparation and analysis of a catalyst and measurements of changes in the concentration of COD in the MFC with a Ni–Co cathode. Analysis of the catalyst included measurements of the electroless potential of Ni–Co electrodes oxidized for 1–10 h, and the influence of anodic charge on the catalytic activity of the Ni–Co alloy (for four alloys: 15, 25, 50, and 75% concentration of Co). For the Ni–Co alloy containing 15% of Co oxidized for 8 h, after the third anodic charge the best catalytic parameters was obtained. During the MFC operation, it was noted that the COD reduction time (to 90% efficiency) was similar to the reduction time during wastewater aeration. However, the characteristic of the aeration curve was preferred to the curve obtained during the MFC operation. The electricity measurements during the MFC operation showed that power equal to 7.19 mW was obtained (at a current density of 0.47 mA·cm−2).
Natural Naphthohydroquinone Dimer Rubioncolin C Exerts Anti-Tumor Activity by Inducing Apoptotic and Autophagic Cell Death and Inhibiting the NF-κB and Akt/mTOR/P70S6K Pathway in Human Cancer Cells
Authors: Wang Jia , Li Ling , Wang Jing , Song Lihua , Tan Ninghua , Wang Zhe
Published: December 2019

Naphthohydroquinone dimers isolated from Rubia plants have garnered more attention due to their distinctive chemical structures and intriguing bioactivities. In our previous studies, we obtained ten naphthohydroquinone dimers containing seven novel ones and found that most of them possessed anti-tumor activities, especially rubioncolin C. However, the underlying mechanism remains unknown. In this study, we focused on rubioncolin C and found that it could inhibit the growth of cancer cell lines with IC50 values between 1.14 and 9.93 μM. Further experiments demonstrated that rubioncolin C induced apoptotic and autophagic cell death and inhibited the Akt/mTOR/P70S6K signaling pathway in HCT116 and HepG2 cells. Moreover, we observed that rubioncolin C inhibited the TNF-α- and LPS-induced NF-κB activation upstream of the p65 protein, which contributed to rubioncolin C-induced cell death. Rubioncolin C could also prevent LPS-induced endotoxin shock in vivo. Moreover, rubioncolin C suppressed tumor growth through inducing apoptosis and autophagy and inactivating NF-κB in vivo. These findings clarify the anti-tumor mechanism of rubioncolin C using biochemical techniques and pharmacological models and might contribute to the future development of rubioncolin C as a new therapeutic agent for treating cancer.
The Upstream Pathway of mTOR-Mediated Autophagy in Liver Diseases
Authors: Wang Haojie , Liu Yumei , Wang Dongmei , Xu Yaolu , Dong Ruiqi , Yang Yuxiang , Lv Qiongxia , Chen Xiaoguang , Zhang Ziqiang
Published: December 2019

Autophagy, originally found in liver experiments, is a cellular process that degrades damaged organelle or protein aggregation. This process frees cells from various stress states is a cell survival mechanism under stress stimulation. It is now known that dysregulation of autophagy can cause many liver diseases. Therefore, how to properly regulate autophagy is the key to the treatment of liver injury. mechanistic target of rapamycin (mTOR)is the core hub regulating autophagy, which is subject to different upstream signaling pathways to regulate autophagy. This review summarizes three upstream pathways of mTOR: the phosphoinositide 3-kinase (PI3K)/protein kinase (AKT) signaling pathway, the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway, and the rat sarcoma (Ras)/rapidly accelerated fibrosarcoma (Raf)/mitogen-extracellular activated protein kinase kinase (MEK)/ extracellular-signal-regulated kinase (ERK) signaling pathway, specifically explored their role in liver fibrosis, hepatitis B, non-alcoholic fatty liver, liver cancer, hepatic ischemia reperfusion and other liver diseases through the regulation of mTOR-mediated autophagy. Moreover, we also analyzed the crosstalk between these three pathways, aiming to find new targets for the treatment of human liver disease based on autophagy.
Genetic Disorders Associated with Metal Metabolism
Authors: Umair Muhammad , Alfadhel Majid
Published: December 2019

Genetic disorders associated with metal metabolism form a large group of disorders and mostly result from defects in the proteins/enzymes involved in nutrient metabolism and energy production. These defects can affect different metabolic pathways and cause mild to severe disorders related to metal metabolism. Some disorders have moderate to severe clinical consequences. In severe cases, these elements accumulate in different tissues and organs, particularly the brain. As they are toxic and interfere with normal biological functions, the severity of the disorder increases. However, the human body requires a very small amount of these elements, and a deficiency of or increase in these elements can cause different genetic disorders to occur. Some of the metals discussed in the present review are copper, iron, manganese, zinc, and selenium. These elements may play a key role in the pathology and physiology of the nervous system.
Electrochemical Behavior, Microstructure, and Surface Chemistry of Thermal-Sprayed Stainless-Steel Coatings
Authors: Nascimento Alexandre R. C. , Gateman Samantha Michelle , Mauzeroll Janine , Savoie Sylvio , Schulz Robert , Moreau Christian
Published: December 2019

Thermally sprayed stainless-steel coatings were produced with a wide range of deposition parameters. The electrochemical behavior of polished coatings was monitored for 3 weeks in 3.5 wt.% NaCl aqueous solution and compared to that of reference materials including a wrought stainless steel plate and a bulk ingot produced by arc melting of the spraying powder feedstock. Transitions in the polarization behavior are discussed based on the observed changes in coating microstructures as well as on the shifts in X-ray photoelectron spectra (XPS). Results show that the deposition parameters have a strong effect on the coating microstructures but the small differences in the polarization behavior of coatings mostly disappear after 1 week of testing. Microstructure evidence shows preferential corrosion at splats experiencing melting prior to deposition. Pitting and corrosion products between splat boundaries are also reported. XPS analysis shows that the coating surfaces are enriched in chromium oxides and hydroxides. Comparison between the coating and bulk stainless steels suggests that coating inherent defects play a major role on their impaired corrosion resistance.
Origin of Nanoscale Incipient Plasticity in GaAs and InP Crystal
Authors: Chrobak Dariusz , Trębala Michał , Chrobak Artur , Nowak Roman
Published: December 2019

In this article, we exhibit the influence of doping on nanoindentation-induced incipient plasticity in GaAs and InP crystals. Nanoindentation experiments carried out on a GaAs crystal show a reduction in contact pressure at the beginning of the plastic deformation caused by an increase in Si doping. Given that the substitutional Si defects cause a decrease in the pressure of the GaAs-I → GaAs-II phase transformation, we concluded that the elastic–plastic transition in GaAs is a phase-change-driven phenomenon. In contrast, Zn- and S-doping of InP crystals cause an increase in contact pressure at the elastic–plastic transition, revealing its dislocation origin. Our mechanical measurements were supplemented by nanoECR experiments, which showed a significant difference in the flow of the electrical current at the onset of plastic deformation of the semiconductors under consideration.
Bending Nanoindentation and Plasticity Noise in FCC Single and Polycrystals
Authors: Bolin Ryder , Yavas Hakan , Song Hengxu , Hemker Kevin J. , Papanikolaou Stefanos
Published: December 2019

We present a high-throughput nanoindentation study of in situ bending effects on incipient plastic deformation behavior of polycrystalline and single-crystalline pure aluminum and pure copper at ultranano depths (< 200 nm). We find that hardness displays a statistically inverse dependence on in-plane stress for indentation depths smaller than 10 nm, and the dependence disappears for larger indentation depths. In contrast, plastic noise in the nanoindentation force and displacement displays statistically robust noise features, independently of applied stresses. Our experimental results suggest the existence of a regime in Face Centered Cubic (FCC) crystals where ultranano hardness is sensitive to residual applied stresses, but plasticity pop-in noise is insensitive to it.
Synthesis and Characterization of (Ca,Sr)[C2O4]∙nH2O Solid Solutions: Variations of Phase Composition, Crystal Morphologies and in Ionic Substitutions
Authors: Rusakov Aleksei V. , Kuzmina Mariya A. , Izatulina Alina R. , Frank-Kamenetskaya Olga V.
Published: December 2019

To study strontium (Sr) incorporation into calcium oxalates (weddellite and whewellite), calcium-strontium oxalate solid solutions (Ca,Sr)[C2O4]∙nH2O (n = 1, 2) are synthesized and studied by a complex of methods: powder X-ray diffraction (PXRD), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) spectroscopy. Two series of solid solutions, isomorphous (Ca,Sr)[C2O4]·(2.5 − x)H2O) (space group I4/m) and isodimorphous Ca[C2O4]·H2O(sp.gr. P21/c)–Sr[C2O4]·H2O(sp.gr. P 1 - ), are experimentally detected. The morphogenetic regularities of their crystallization are revealed. The factors controlling this process are discussed.
Comparative Study on AC Susceptibility of YBa2Cu3O7−δ Added with BaZrO3 Nanoparticles Prepared via Solid-State and Co-Precipitation Method
Authors: Mohd Hapipi Nurhidayah , Lim Jee , Chen Soo , Lee Oon , Shaari Abdul , Awang Kechik Mohd , Lim Kean , Tan Kar , Murakami Masato , Miryala Muralidhar
Published: December 2019

Polycrystalline samples of YBa2Cu3O7−δ (Y-123) added with x mol% of BaZrO3 (BZO) nanoparticles (x = 0.0, 2.0, 5.0, and 7.0) were synthesized using co-precipitation (COP) and solid-state (SS) method. X-ray diffraction (XRD) patterns showed the formation of Y-123 and Y-211 as the major and minor phases, respectively. The samples prepared using COP method showed higher weight percentage of Y-123 phase (≤98%) compared to the SS samples (≤93%). A peak corresponding to BZO was also found in the samples added with BZO nanoparticles. The increasing intensity of the BZO peak as the BZO amount increased showed the increasing amount of the unreacted nanoparticles in the samples. Refinement of unit cell lattice parameters indicated that all the samples have an orthorhombic crystal structure and there is no orthorhombic-tetragonal phase transformation. As observed using scanning electron microscopy (SEM), all the samples showed randomly distributed grains with irregular shape. The average grain size for the pure sample prepared using COP method is smaller (0.30 µm) compared with that of the pure SS sample (1.24 µm). Addition of 7.0 mol% BZO led to an increase of average grain size to 0.50 μm and 2.71 μm for the COP and SS samples, respectively, indicating grain growth. AC susceptibility (ACS) measurement showed a decrease in the onset critical temperature, Tc-onset with BZO addition. Comparatively, Tc-onset for the COP samples is higher than that of the SS samples. The value of Josephson’s current, Io increased up to 2.0 mol% BZO addition, above which the Io decreased more drastically for the SS samples. The value of Io is 53.95 μA and 32.08 μA for the 2.0 mol% BZO added SS and COP samples, respectively. The decrease of Io is attributed to the distribution of BZO particles at the grain boundaries as also reflected in the drastic decrease of phase lock-in temperature, Tcj. As a result of smaller average grain size, the presence of more grain boundaries containing insulating BZO particles led to lower Io in the COP samples.
Crystallographic Insights into Uranyl Sulfate Minerals Formation: Synthesis and Crystal Structures of Three Novel Cesium Uranyl Sulfates
Authors: Tyumentseva Olga S. , Kornyakov Ilya V. , Britvin Sergey N. , Zolotarev Andrey A. , Gurzhiy Vladislav V.
Published: December 2019

An alteration of the uranyl oxide hydroxy-hydrate mineral schoepite [(UO2)8O2(OH)12](H2O)12 at mild hydrothermal conditions was studied. As the result, four different crystalline phases Cs[(UO2)(SO4)(OH)](H2O)0.25 (1), Cs3[(UO2)4(SO4)2O3(OH)](H2O)3 (2), Cs6[(UO2)2(SO4)5](H2O)3 (3), and Cs2[(UO2)(SO4)2] (4) were obtained, including three novel compounds. The obtained Cs uranyl sulfate compounds 1, 3, and 4 were analyzed using single-crystal XRD, EDX, as well as topological analysis and information-based structural complexity measures. The crystal structure of 3 was based on the 1D complex, the topology of which was unprecedented for the structural chemistry of inorganic oxysalts. Crystal chemical analysis performed herein suggested that the majority of the uranyl sulfates minerals were grown from heated solutions, and the temperature range could be assumed from the manner of interpolyhedral linkage. The presence of edge-sharing uranyl bipyramids most likely pointed to the temperatures of higher than 100 °C. The linkage of sulfate tetrahedra with uranyl polyhedra through the common edges involved elevated temperatures but of lower values (~70–100 °C). Complexity parameters of the synthetic compounds were generally lower than that of uranyl sulfate minerals, whose structures were based on the complexes with the same or genetically similar topologies. The topological complexity of the uranyl sulfate structural units contributed the major portion to the overall complexity of the synthesized compounds, while the complexity of the respective minerals was largely governed by the interstitial structure and H-bonding system.
Symmetric Fluoroborate and its Boron Modification: Crystal and Electronic Structures
Authors: Dziuk Błażej , Ośmiałowski Borys , Zarychta Bartosz , Ejsmont Krzysztof , Chęcińska Lilianna
Published: December 2019

Four boron-carrying molecules were synthesized and purified. These were found to be (a) relatively neutral with respect to the parent BF derivative and (b) functionalized by donor–acceptor groups resulting in a charge transfer within the molecule. The study discusses the steric effect and the influence of the substitution of the side rings on the surroundings of the boron atom. Electronic structures were characterized by real-space bonding indicators. Hirshfeld surface and energy frameworks tools were applied to examine the crystal packing features.
Virtual Mannequin Simulation for Customized Electromagnetic Shielding Maternity Garment Manufacturing
Authors: Javadi Toghchi Marzieh , Bruniaux Pascal , Campagne Christine , Cayla Aurélie , Loghin Carmen , Cristian Irina , Chen Yan , Wang Lichuan
Published: December 2019

Trying virtual garments on a virtual body has become widespread in the design industry as a result of 3D computer-aided design (CAD) system and progress in body measurement systems. Spending less time and cost compared to traditional methods of garment design is the main benefit of design software development. Moreover, it leads to improved garment fitting on the body which fulfills customer desires. In the present study, a parametric graphical method was utilized to develop a 3D virtual mannequin for the pregnancy period. The former, the 3D adaptive mannequin model was predicted based on a woman’s weight by analyzing body morphology evolution of a pregnant woman. The mannequin was verified by the results of another pregnant body. Then, the developed mannequin based on weight gain during maternity was applied to design a garment block pattern. The virtual try-on of the developed garment block pattern suggested that it was well-fitted on the 3D virtual mannequin while two armpit darts and two elastic seams were allocated. Hence, the developed garment block pattern will be used to make a personalized protective garment in our future work using an electromagnetic shielding woven fabric formerly manufactured by our group.
Correlation between Spot Sign and Intracranial Hemorrhage Expansion on Dual-Phase CT Angiography
Authors: Kim Hyesoo , Goo Ja Hong , Kwak Hyo Sung , Hwang Seung Bae , Chung Gyung Ho
Published: December 2019

Purpose: Expansion of intracranial hemorrhage (ICH) is an important predictor of poor clinical outcome. ICH expansion can be predicted with a spot sign on computed tomographic angiography (CTA). We aimed to evaluate the correlation between spot signs on CTA and ICH expansion on dual-phase CTA. Methods: Patients with spontaneous ICH between January 2017 and April 2019 who underwent an initial CT, dual-phase CTA, and a subsequent CT were retrospectively identified. ICH expansion was defined as volume growth of >33% or >6 mL. We analyzed the presence and change in size of the spot sign in the first phase and second phase CTA. Also, we divided the morphological status of the spot sign, such as a dot-like lesion or linear contrast extravasation, in the first and second phase CTA. Results: A total of 206 patients, including 38 (18.5%) with ICH expansion and 45 (21.8%) with a spot sign, qualified for analysis. Of patients with a spot sign, 26 (57.8%) had ICH expansion on subsequent CT. Increased size of a spot sign in second-phase CTA was more frequent in the ICH expansion group than in the no-expansion group (96.2% vs. 52.6%, p < 0.001). First visualization of a spot sign in the second phase was more common in the no-expansion group than in the ICH expansion group (47.4% vs. 3.8%, p < 0.001). The morphological patterns of a spot sign between the two groups were not significantly different. Conclusion: Spot signs on dual-phase CTA have different sizes and morphological patterns. Increased size of a spot sign in the second phase of CTA can help identify patients at risk for ICH expansion.
Noncoding RNAs and Liquid Biopsy in Lung Cancer: A Literature Review
Authors: Haranguș Antonia , Berindan-Neagoe Ioana , Todea Doina Adina , Șimon Ioan , Șimon Mărioara
Published: December 2019

Lung cancer represents a genetically heterogeneous disease with low survival rates. Recent data have evidenced key roles of noncoding RNAs in lung cancer initiation and progression. These functional RNA molecules that can act as both oncogenes and tumor suppressors may become future biomarkers and more efficient therapeutic targets. In the precision medicine era, circulating nucleic acids have the potential to reshape the management and prognosis of cancer patients. Detecting genomic alterations and level variations of circulating nucleic acids in liquid biopsy samples represents a noninvasive method for portraying tumor burden. Research is currently trying to validate the potential role of liquid biopsy in lung cancer screening, prognosis, monitoring of disease progression, and treatment response. However, this method requires complex detection assays, and implementation of plasma genotyping in clinical practice continues to be hindered by discrepancies that arise when compared to tissue genotyping. Understanding the genomic landscape of lung cancer is essential in order to provide useful and innovative research in the age of patient-tailored therapy. In this landscape, the noncoding RNAs play a crucial role due to their target genes that dramatically influence the tumor microenvironment and the response to therapy. This article addresses present and future possible roles of liquid biopsy in lung cancer. It also discusses how the complex role of noncoding RNAs in lung tumorigenesis could influence the management of this pathology.
Modeling Expected Shortfall Using Tail Entropy
Authors: Pele Daniel Traian , Lazar Emese , Mazurencu-Marinescu-Pele Miruna
Published: December 2019

Given the recent replacement of value-at-risk as the regulatory standard measure of risk with expected shortfall (ES) undertaken by the Basel Committee on Banking Supervision, it is imperative that ES gives correct estimates for the value of expected levels of losses in crisis situations. However, the measurement of ES is affected by a lack of observations in the tail of the distribution. While kernel-based smoothing techniques can be used to partially circumvent this problem, in this paper we propose a simple nonparametric tail measure of risk based on information entropy and compare its backtesting performance with that of other standard ES models.