bluuebunny/crossref_metadata_2025 huggingface dataset:
Association between oxidative balance score and serum cobalt level in population with metal implants: a cross-sectional study from NHANES 2015–2020
Authors: Yuan Wenxiu , Chen Jing , Sun Jun , Song Chenyang , Chen Zhi
Published: December 2024

Background

Growing evidence indicates that metal implants influence the body’s oxidative stress status, which in turn affects the degradation and stability of metal implants. The oxidative balance score (OBS) is a composite indicator, reflecting the overall oxidative balance of pro-and antioxidants of the human body. However, the associations between OBS and the level of metal ions on the population with metal implants remain to be elucidated.

Methods

We conducted a cross-sectional study using data from 2015 to 2020 National Health and Nutrition Examination Survey (NHANES). Dietary and lifestyle factors closely associated with oxidative stress were quantified to calculate the OBS. Weighted multivariate logistic regression and smooth curve fittings were performed to examine the relationship between OBS and serum cobalt levels. Subgroup analyses were stratified by age and gender. In cases where non-linearity was detected, threshold effects were assessed using a two-piecewise linear regression model.

Results

A total of 549 participants were included in this analysis. The dietary OBS was negatively associated with serum cobalt level in fully adjusted model (β = −0.179, 95%CI: −0.358 to −0.001, P: 0.04918). Stratified by age and gender, negative correlation of OBS and dietary OBS with serum cobalt level was observed only in men and age over 70 years participants. Threshold effect analysis showed linear relationships between OBS, dietary OBS and cobalt level in males. There were non-linear relationships between OBS, dietary OBS and cobalt level in age over 70 years participants, with inflection points identified at 16.3 and 8.7 for OBS and dietary OBS, respectively.

Conclusion

Our study confirms the inverse relationships between oxidative stress and serum cobalt level in individuals with metal implants, highlighting the significance of optimizing OBS to mitigate the risk of metal ion toxicity. These findings emphasize the importance of maintaining an antioxidant diet and lifestyle, particularly as they offer greater protective effect for males and the elderly population.
An intracranial yolk sac tumor with initial symptom of hemorrhage in the basal ganglia: a case report
Authors: Liu Qiang , Du Xuecui , Wang Yunyan , Hou Ruihua , Chen Yuqing , Chen Teng
Published: December 2024

We report a case and follow-up of an adult male with intracranial yolk sac tumor (YST). Initially, the patient presented with abnormal high signals in the right basal ganglia on MRI, misdiagnosed as a cavernous hemangioma. However, within 2 years, the condition rapidly progressed into a large, hypervascular solid neoplasm leading to a basal ganglia hemorrhage. Comprehensive evaluation of clinical symptoms, imaging, surgical findings, serology, histopathology, and genetic analysis confirmed the diagnosis of a yolk sac tumor. The patient underwent prompt surgical resection followed by radiotherapy and chemotherapy. Six months post-treatment, his condition remains stable, with no recurrence. Notably, early MRI revealed Wallerian degeneration in the brainstem suggesting that benign-appearing basal ganglia lesions may sometimes result from malignant infiltration by germ cell tumors or other cancers. To prevent misdiagnosis and ensure timely treatment, a stereotactic biopsy is recommended. We hope this case provides a valuable reference for diagnosing and treating YSTs and contributes to ongoing research aimed at improving patient survival.
Postoperative complications after central nervous system tumor resection in pediatric patients admitted to an intensive care unit in Colombia
Authors: Lasso-Palomino Rubén E. , Gómez Inés Elvira , Soto-Aparicio María José , Gempeler Andrés , Pombo-Jiménez Andrés , Gómez-Toro Melissa , Rojas-Robledo Valentina , Jiménez-Arévalo María Alejandra , Bastidas-Toro Karla Alejandra , Sierra Jimena , Martínez-Betancur Sofía , Ariza-Insignares Camila , Montaño-Vivas Isabella , Castro Ximena , Arias Anita V.
Published: December 2024

Introduction

Central nervous system (CNS) tumors are the second most prevalent malignant neoplasms in childhood, with surgical resection as the primary therapeutic approach. The immediate postoperative period following CNS tumor resection requires intensive care to mitigate complications associated with high morbidity and mortality.

Objective

The primary aim of this study is to comprehensively describe the postoperative complications observed in pediatric patients who underwent primary CNS tumor resection and were subsequently admitted to the pediatric intensive care unit (PICU) at Hospital Universitario Fundación Valle del Lili in Colombia.

Methods

We conducted a cross-sectional observational analysis of pediatric patients who underwent surgery for CNS tumors and were admitted to our PICU from January 2011 to December 2021. Clinical, histopathologic, and postoperative complication data were collected. A descriptive statistical analysis was performed using measures of dispersion and central tendency with a 95% confidence interval.

Results

A total of 114 patients were included, of whom 55.3% were male. The median PICU stay was 4 days (2–7). The most common tumor type was embryonal (25.4%), followed by low-grade glioma (20.1%) and high-grade glioma (14.9%). Mechanical ventilation was required in 24.5% of patients, with a median extubation time of 3 days (2–9). In the immediate postoperative period, 6.14% of patients experienced CNS hemorrhage and 3.5% experienced intracranial hypertension. Common complications included motor deficits, facial paralysis, and sensory deficits. The mortality rate was 3.5%.

Conclusion

This study describes the postoperative complications, clinical challenges, and interventions observed in pediatric patients after CNS tumor resection in a resource-limited country. Our findings emphasize the importance of tailored interventions and multidisciplinary collaboration to optimize clinical outcomes. Future data comparison from centers sharing similar characteristics will play a crucial role in identifying best practices and enhancing outcomes globally.
Protective effect of Haoqin Qingdan decoction on pulmonary and intestinal injury in mice with influenza viral pneumonia
Authors: Lin Xi , Lin Jian , Ji Lichun , Zhang Jiaona , Zhang Yezi , Hong Junbin , Li Geng , Lin Xingdong
Published: December 2024

Background

Haoqin Qingdan decoction (HQQD), composed of eleven herbs, is a traditional Chinese formula widely recognized for its efficacy in treating pulmonary inflammation induced by viral infections. Despite its extensive use, the potential pulmonary and intestinal protective effects of HQQD on influenza viral pneumonia (IVP) and the underlying molecular mechanisms remain unclear.

Materials and Methods

Ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS) was employed to identify the major chemical constituents of the prescription. Subsequently, network analysis was conducted to predict the potential therapeutic targets of HQQD in IVP. The mechanisms by which HQQD mitigates lung and intestinal damage were further elucidated by assessing NP protein expression, inflammatory factors, TLR7/MyD88/NF-κB signaling pathway mRNAs and proteins, and through intestinal flora analysis.

Results

The protective effects of HQQD on pulmonary and intestinal injuries induced by IVP were thoroughly investigated using comprehensive network analysis, signaling pathway validation, and gut microflora analysis. UHPLC-MS analysis identified the primary chemical constituents. Validation experiments demonstrated a significant reduction in NP protein expression in the lungs. HQQD notably alleviated immune damage in the lungs and intestines of mice by inhibiting NP protein expression and the release of inflammatory factors such as interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ); downregulating the expression levels of TLR7, MyD88, and phospho-NF-κB p65 (p-p65); lowering serum LPS levels; and reducing the relative abundance of Proteobacteria.

Conclusion

HQQD exerts therapeutic effects against influenza viral pneumonia through antiviral and anti-inflammatory mechanisms and by remodeling the intestinal flora. This study provides initial insights into the “gut-lung” axis mechanism of HQQD in combating respiratory influenza virus infection.
Despite the pandemic: upward trajectories of medication adherence and persistence in patients with dyslipidemia
Authors: Jang Jieun , Oh Hyun Jung , Lee Eui-Kyung
Published: December 2024

Background

Dyslipidemia, a major cardiovascular risk factor, requires consistent medication adherence, but new patients often struggle due to its asymptomatic nature. The COVID-19 pandemic has disrupted global healthcare. This study examined its impact on medication adherence and persistence among Korean patients with dyslipidemia (PWD), comparing the effects on new versus existing PWD.

Method

Nationwide claims data were used to identify PWD and their prescribed medications. Patients were categorized as new or existing PWD and matched 1:1 using propensity scores in both the pre- and post-COVID-19 periods. Medication adherence was measured by the proportion of days covered (PDC), and persistence was assessed by analyzing gaps in continuous medication dispensing. The impact of COVID-19 was evaluated using multiple regression and Cox proportional hazard models.

Result

The pre-COVID-19 cohort included 519,696 patients, and the post-COVID-19 cohort comprised 536,762 patients. PDC significantly increased post-COVID-19, with existing PWD showing a larger increase by 4.74 units (p < 0.0001), compared with 2.01 units for new PWD (p < 0.0001). Both groups exhibited lower risks of medication discontinuation, with a greater decrease observed in the existing PWD (hazard ratio [HR] 0.780, 95% confidence interval [CI] [0.774–0.786], p < 0.0001), compared with the new PWD (HR 0.929, 95% CI [0.923–0.934], p < 0.0001). New PWD had fewer annual visits, whereas existing PWD had more visits (both p < 0.0001).

Conclusion

Despite the COVID-19 pandemic, medication adherence and persistence improved in both new and existing PWD. Notably, new patients with no prior treatment experience showed weaker positive responses, highlighting the potential need for targeted interventions to support new patients during public health crises.
Research progress and prospect of key technologies of fruit target recognition for robotic fruit picking
Authors: Liu Shaohua , Xue Jinlin , Zhang Tianyu , Lv Pengfei , Qin Huanhuan , Zhao Tianxing
Published: December 2024

It is crucial for robotic picking fruit to recognize fruit accurately in orchards, this paper reviews the applications and research results of target recognition in orchard fruit picking by using machine vision and emphasizes two methods of fruit recognition: the traditional digital image processing method and the target recognition method based on deep learning. Here, we outline the research achievements and progress of traditional digital image processing methods by the researchers aiming at different disturbance factors in orchards and summarize the shortcomings of traditional digital image processing methods. Then, we focus on the relevant contents of fruit target recognition methods based on deep learning, including the target recognition process, the preparation and classification of the dataset, and the research results of target recognition algorithms in classification, detection, segmentation, and compression acceleration of target recognition network models. Additionally, we summarize the shortcomings of current orchard fruit target recognition tasks from the perspectives of datasets, model applicability, universality of application scenarios, difficulty of recognition tasks, and stability of various algorithms, and look forward to the future development of orchard fruit target recognition.
Mapping rapeseed (Brassica napus L.) aboveground biomass in different periods using optical and phenotypic metrics derived from UAV hyperspectral and RGB imagery
Authors: Sun Chuanliang , Zhang Weixin , Zhao Genping , Wu Qian , Liang Wanjie , Ren Ni , Cao Hongxin , Zou Lidong
Published: December 2024

Aboveground biomass (AGB) is a key indicator of crop nutrition and growth status. Accurately and timely obtaining biomass information is essential for crop yield prediction in precision management systems. Remote sensing methods play a key role in monitoring crop biomass. However, the saturation effect makes it challenging for spectral indices to accurately reflect crop changes at higher biomass levels. It is well established that rapeseed biomass during different growth stages is closely related to phenotypic traits. This study aims to explore the potential of using optical and phenotypic metrics to estimate rapeseed AGB. Vegetation indices (VI), texture features (TF), and structural features (SF) were extracted from UAV hyperspectral and ultra-high-resolution RGB images to assess their correlation with rapeseed biomass at different growth stages. Deep neural network (DNN), random forest (RF), and support vector regression (SVR) were employed to estimate rapeseed AGB. We compared the accuracy of various feature combinations and evaluated model performance at different growth stages. The results indicated strong correlations between rapeseed AGB at the three growth stages and the corresponding indices. The estimation model incorporating VI, TF, and SF showed higher accuracy in estimating rapeseed AGB compared to models using individual feature sets. Furthermore, the DNN model (R2 = 0.878, RMSE = 447.02 kg/ha) with the combined features outperformed both the RF (R2 = 0.812, RMSE = 530.15 kg/ha) and SVR (R2 = 0.781, RMSE = 563.24 kg/ha) models. Among the growth stages, the bolting stage yielded slightly higher estimation accuracy than the seedling and early blossoming stages. The optimal model combined DNN with VI, TF, and SF features. These findings demonstrate that integrating hyperspectral and RGB data with advanced artificial intelligence models, particularly DNN, provides an effective approach for estimating rapeseed AGB.
Turning stories into learning journeys: the principles and methods of Immersive Education
Authors: Brunetti Riccardo , Ferrante Silvia , Avella Anna Maria , Indraccolo Allegra , Del Gatto Claudia
Published: December 2024

This paper describes the theoretical and practical aspects of Immersive Education, an educational methodology based on interactive narratives, articulated as emotional journeys, to develop competencies. It has been developed throughout three school years (2021–2024) with more than 400 students (8–12 years old) in Public Schools in Italy and Spain. Immersive Education can be integrated with curricular school activities and can be used to target both curricular and transversal learning objectives, specifically the ones connected with the Personal, Social and Learning to learn Key Competence (LifeComp European framework). The paper describes the inspirations that led to the creation of the methodology, including similar experiential learning approaches. It then analyses the theoretical principles of the methodology, dividing them in four key-concepts, along with psychological evidence supporting them. The four key-concepts describe how immersive education aims at being a motivation trigger, featuring a dramatic structure, how it is based on the involvement of the self, and how it focuses on fostering a continuous engagement. It continues with a detailed analysis of implementation strategies, specifically about the management of emotional triggers and reactions, enriched by numerous examples taken from the projects implemented with the students. The conclusions open the way to future research directions to measure the impact of this approach on the development of transversal and specific competences.
Psychometric properties of the pain anxiety symptom scale among postoperative patients in Amol, Iran
Authors: Sharif-Nia Hamid , Froelicher Erika Sivarajan , Marôco João , Hoseinzadeh Esmaeil , Hejazi Sima , Fatehi Reza , Nowrozi Poorya , Mohammadi Bita
Published: December 2024

Introduction

So far, the psychometric properties of the Persian version of the Pain Anxiety Symptom Scale have not been assessed in Iran. Therefore, this study was conducted to determine the validity and reliability of the Persian version of the Pain Anxiety Symptom Scale among a group of Iranian patients in Amol.

Methods

This methodological study was conducted in 2023 with a sample of 400 postoperative patients from Amol, Iran, selected through convenience sampling. The dataset was divided into two groups of 200 for exploratory and confirmatory factor analyses. Construct validity was assessed using maximum likelihood exploratory factor analysis with Promax rotation, supported by Horn’s parallel analysis and network analysis to visualize item relationships. Confirmatory factor analysis, convergent and discriminant validity was performed on the second dataset. Reliability was evaluated through various statistical measures, including Cronbach’s alpha, McDonald’s omega, average inter-item correlation coefficient, composite reliability, and maximal reliability (MaxR).

Results

Among the 400 participants, the mean age was 44.38 years (SD = 13.49), with 152 (46.1%) being women and 178 (53.9%) men. Most participants (n = 268, 81.2%) had an education level lower than a diploma, and 93 individuals (28.2%) reported a history of surgery. The results of exploratory factor analysis with Promax rotation developed two factors accounting for 66.29% of the variance comprising 15 items. Also, after necessary modifications during confirmatory factor analysis, the final model was approved. As for reliability, the Cronbach’s alpha, composite reliability, and MaxR for all constructs were greater than 0.7, demonstrating good internal consistency and construct reliability.

Conclusion

According to the results, the Persian version of the Pain Anxiety Symptom Scale has a valid structure and acceptable reliability. This scale can be used by health professionals in many ways.
Placebo and the law of identification
Authors: Bierman Steve F. , Weil Andrew , Dahmer Stephen
Published: December 2024

Thousands of essays and studies have been published on placebo and nocebo. Yet, despite this plethora of information, we are not much closer to a comprehensive understanding of the fundamental mechanism producing placebo and nocebo effects than we were in 1946, when participants in the Cornell Conferences on Therapy speculated on the roles of authority, belief and expectancy. In this paper, we examine the weaknesses in current placebo and nocebo definitions and theories. We also propose a more concise and comprehensive definition and theory of placebo and nocebo by introducing the Law of Identification and the Generic Placebo Instruction (GPI). The latter being the placebo/nocebo information expressed or implied in virtually every clinical encounter and trial; the former (i.e., the Law of Identification), being what drives the GPI to actualization. Further, we demonstrate the explanatory power of this new theory and suggest clinical studies that test predictions arising from it - studies whose results, if positive, would translate universally into clinical practice.
Vaccination: a look at the social representations of Brazilian children
Authors: de Gaspi Suelen , Magalhães Júnior Carlos Alberto de Oliveira , Tracana Rosa Branca , Schneider Eduarda Maria , Carvalho Graça S.
Published: December 2024

Introduction

The pandemic caused by COVID-19 has accentuated the debate on the need for vaccination and called into question the need to increasingly bring this topic, which is widely disseminated in the scientific world, to school classes at all schooling phases. In this scenario, science education plays a key role in disseminating knowledge about the importance of vaccination and the impacting factors of a lack of immunization. In order to better understand this movement, it is necessary to understand the representations of individuals as a way of broadening paths to change this scenario.

Objectives

This study aimed to identify and analyse Brazilian primary school children’s social representations of vaccination.

Methods

Using the free word recall technique, the term “vaccination” was applied to evoke children’s ideas. The analysis of co-occurrences of evocations permitted us to identify their representations’ centrality.

Results

The results showed that the centralizing elements guiding these children’s social representations were “needle,” “pain,” and “health center.”

Conclusion

These results show the need to prevent the phobia of needles that arises in the first vaccination experiences and reinforce the importance of discussing the subject of vaccination in science teaching. This issue is even more critical, given the spread and impact of “fake news” on social media. There is an increasing need to emphasize the importance of vaccination, not only as a factor of individual protection but also as a commitment to collective health. This study also showed that the school, as usual, must become an ally in tackling this reality with children and their families.
Availability of eye health interventions in basic schools in a Ghanaian municipality
Authors: Adzaho Christopher Senyo , Appiah-Brempong Emmanuel , Acheampong Princess Ruhama , Ekutor Ernest
Published: December 2024

Introduction

Globally, 19 to 22 million children are visually impaired. A huge number of children therefore will not be able to learn effectively even if the best educational institutions are made available to them. This ultimately leaves a potential negative effect on their quality of life, educational opportunities and livelihoods. School health programs (SHPs) present a unique opportunity to provide comprehensive eye health services to children. This study assessed the availability of School Eye Health Programs (SEHPs) in a municipality in Ghana.

Design and methods

In a cross-sectional study, semi-structured questionnaires and interview guides were used to collect data from 60 head teachers, 17 school health coordinators, and 7 key health workers.

Results

No school in the municipality had a comprehensive SEHP being implemented. However, over 60% of schools had at some point introduced at least one component of SEHP, mainly Vitamin A supplementation and vision screening. Community and Public Health Nurses are the commonly used human resource for such programs. There were no available guidelines for program implementation and no systems in place for ensuring follow-up visits and provision of spectacles to those with refractive errors. Key barriers identified included the lack of financial resources and adequately trained personnel.

Conclusion

Comprehensive school eye health programs are unavailable in the study area and there is an urgent need for their implementation to avert the potential adverse effects of vision impairment on the education of children.
Energy efficiency and system complexity analysis of CNN based hybrid precoding for cell-free massive MIMO under terahertz communication
Authors: Abose Tadele A. , Mekonen Yitbarek A. , Assefa Binyam G. , Gudeta Naol W.
Published: December 2024

The integration of terahertz (THz) communication with cell-free massive multiple-input multiple-output (CFMM) systems presents a promising strategy to enhance energy efficiency and reduce system complexity in future wireless networks. However, this integration faces significant challenges, such as dynamic and unpredictable channel behavior. Traditional channel estimation techniques are inadequate for handling these dynamic conditions. To address these issues, a convolutional neural network (CNN)-based hybrid precoding scheme is proposed for CFMM systems operating at THz frequencies. This method leverages CNN to predict optimal precoding weights, significantly improving the adaptability of hybrid precoding. The CNN-based model not only mitigates pilot contamination (PC) but also enhances channel estimation by capturing temporal and spatial dynamics. Simulation results indicate that the CNN-based approach achieves superior energy efficiency and lower system complexity compared to conventional techniques. At a signal-to-noise ratio (SNR) of 30 dB, it achieves 1.2 bits per joule and reduces system complexity to 1,400 FLOPs, demonstrating better scalability and resource optimization. These findings highlight the potential of CNN-based hybrid precoding to revolutionize THz communication in next-generation wireless networks by optimizing energy efficiency and system complexity.
Comparison of 6 cycles of isatuximab with lenalidomide, bortezomib and dexamethasone (I-VRd) versus 3 cycles of I-VRd followed by one cycle of high-dose melphalan in newly diagnosed low-risk multiple myeloma. Protocol for a multicenter, prospective, randomized, phase II clinical trial (ELIAS-Trial)
Authors: Leitner Theo , Shumilov Evgenii , Schwitlick Christina , Koch Raphael , Hamm Franziska , Högner Marion , Bassermann Florian , Weisel Katja , Einsele Hermann , Kortüm Martin , Rasche Leo , Görner Martin , Wegehenkel Kai , Knop Stefan , Krönke Jan , Nogai Axel , König Inke R. , Vens Maren , Horn Kay , von Bubnoff Nikolas , Khandanpour Cyrus
Published: December 2024

Newly diagnosed multiple myeloma patients who are eligible for transplant usually receive several induction cycles of therapy, followed by one or two cycles of high-dose melphalan and autologous stem cell transfusion. In myeloma patients, high-dose melphalan improves overall survival and progression-free survival. However, melphalan exposure increases the risk of secondary malignancies and may lead to the transformation of residual myeloma cells into more aggressive clones, which may accelerate relapse. It remains to be determined whether low-risk patients also derive additional benefit from high-dose melphalan therapy compared with less toxic regimens. Here we publish the study protocol of a multicenter, interventional, controlled, randomized, prospective and open-label phase II trial to investigate whether patients with a low-risk profile (R-ISS stage I, characterized by a low tumor burden and the absence of negative cytogenetic findings or elevated LDH levels) and a standard-risk gene expression profile (using the SKY92 GEP assay) can be sufficiently treated with intensified consolidation regimens without prior high-dose melphalan chemotherapy. The primary objective is to assess whether three cycles of isatuximab, bortezomib, lenalidomide and dexamethasone (I-VRd) followed by stem cell apheresis and three additional cycles of I-VRd will result in a non-inferior rate of complete remission (CR) combined with MRD-negativity at week 40 after the start of induction therapy compared to three cycles of I-VRd followed by standard of care treatment (such as stem cell apheresis, high-dose melphalan, and autologous stem cell transplantation). We hypothesize that this approach could reduce toxicity, cost of treatment and the likelihood of the development of a more malignant plasma cell clone, while improving overall survival (OS) and progression-free survival (PFS) in newly diagnosed low risk myeloma patients.

EU Trial Number

2022-500453-16-00, https://clinicaltrials.gov/study/NCT05665140, identifier NCT05665140. Registration Date: 21.07.2022.
Rotations of teeth—a systematic review
Authors: Parthiban Ramya , Kailasam Vignesh , Venkatasamy Nivetha Shree
Published: December 2024

Background

Rotations are frequently evaluated through various assessment methods of crowding and arch dimension, with relatively few studies discussing the extent or direction of rotations and even fewer addressing the reliability of such assessments. This systematic review aims to comprehensively analyze existing classification systems for rotated teeth and assess rotation in anterior and posterior teeth, its clinical applicability, and its impact on retention and relapse.

Search methods

Two investigators conducted a comprehensive search in six databases, namely, PubMed, Scopus, Ovid, LILACS, Web of Science, and Cochrane CENTRAL, up to 28 March 2024. No specific start date was defined to ensure the inclusion of all relevant studies from the inception of each database, maximizing the comprehensiveness of our review. The search criteria included retrospective studies and the inclusion criteria were patients who were assessed for rotation in any age group. The exclusion criteria were patients who had undergone orthodontic treatment, who had fractured restorations or crowns, or who had any other tooth anomaly.

Data collection and analysis

In total, 10 studies satisfying the inclusion criteria were included and 9 provided quantitative outcomes for the rotation of various teeth, while the remaining study offered qualitative results. The risk of bias assessment was performed with the help of the Newcastle–Ottawa quality assessment tool.

Results

The skeletal Class II and Class III groups exhibited similar average positions of the first molar. Upper molar rotation was primarily observed in dental Class II patients, with a higher mesial rotation angle of 78.6°. Only one study measured the rotation for all permanent teeth. Seven studies used the mid-palatal raphe as the reference line for measuring molar rotation. No gender differences were found. It was found that there was no statistical significance in the mean values of molar rotation for the right and left sides as well as the maxillary and mandibular arches. The incisors demonstrated the highest degree of rotation (7.4°–20.2°), while the premolars and canines exhibited a slightly lower degree of rotation (3.3°–9.2°). In contrast, the molars displayed the lowest degree of rotation (0.8°–7.4°).

Conclusion

After reviewing all the studies, it was found that there is no adequate classification system to assess the rotation of anterior teeth and mandibular teeth. A universally accepted classification of tooth rotation, including a common reference line, is needed. The existing systems for posterior teeth need to be standardized and have a clinical utility to be widely accepted.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42024524654, PROSPERO (CRD42024524654).
Vascular reconstructions in living donor kidney transplantation: a single-center experience over the last 17 years
Authors: Roth Nadina , Kalteis Manfred , Krause Axel , Rösch Christiane Sophie , Huber Jürgen , Enkner Wolfgang , Haller Maria , Cejka Daniel , Függer Reinhold , Biebl Matthias
Published: December 2024

Introduction

In living donor kidney transplantation (LDKT), vascular anastomosis is more difficult due to missing arterial patches and shorter renal veins. The surgical challenge is even more demanding in kidneys with multiple arteries. Although renal transplantation is feasible in most cases of complex donor vascular anatomy and similar results compared with standard LDKT are reported, the discussion on potentially increased complication rates and graft function continues. This prompted us to review our results of LDKT with multiple renal artery (MRA) grafts with a special concentration on complications and long-term function.

Patients and methods

We reviewed the records of all LDKT in our center from the beginning of the program in 2005 until 2022 for arterial vascular reconstructions. The cohort was divided into two groups: transplantation with vascular reconstruction (VR) and standard transplantation. These groups were compared for operative parameters and short- and long-term results.

Results

From 2005 to 2022, 211 LDKT were completed in our unit. In 32 (15.2%), a VR was performed, including single ostium side-to-side anastomosis, end-to-side anastomosis, patch reconstruction, and vein interposition. There was no significant difference in operative time (169 min vs. 180 min; p = 0.118) and time for anastomosis (28 min vs. 26 min; p = 0.59) between both groups. Postoperative complications (5.7% vs. 7.4%; p = 0.72) were not significantly different. During the follow-up period (110 months, range 10–204), the risk of organ loss was comparable after VR (13.625% vs. 11.56% p = 0.69).

Conclusion

In LDKT, arterial vascular reconstructions for kidneys with MRA provide similar results compared to grafts with a single renal artery (SRA). Short- and long-term results are comparable with standard procedures.
Transcriptome-wide association studies identify candidate genes for carcass and meat traits in meat rabbits
Authors: He Hua , Tian Xinyang , Kang Zhe , Wang Guozhi , Jia Xianbo , Sun Wenqiang , Lai Song-Jia , Chen Shi-Yi
Published: December 2024

Meat rabbits are a small herbivorous livestock and have been popularly raised in China for producing high-quality meat. Therefore, it is economically important to genetically improve both carcass performance and meat quality in meat rabbits. However, we still know less about the underlying candidate genes that may determine phenotypic variation on carcass and meat traits of meat rabbits. The main objective of this study was to identify candidate genes whose mRNA expression levels may be significantly involved in regulating carcass and meat traits of meat rabbits based on the transcriptome-wide association studies (TWAS). Five carcass traits of the carcass weight (CW), dressing out percentage (DP), cut weight of hind legs (LW), weight ratio of cut hind legs to carcass (RLW), and weight of visceral and interscapular fat (WF), as well as two meat traits of the drip loss (DL) and cooking loss (CL) were phenotyped in a F1 crossbred population (N = 119) between Zika rabbits and Sichuan White rabbits. The effects of mRNA expression levels of a total of 10,288 genome-wide genes on these seven traits were statistically estimated using the mixed linear model, in which the polygenic background effects were accounted for. Our results revealed two candidate genes (RDH5 and MTARC2) that were statistically significantly associated with LW trait (the adjusted p values <0.05), whereas no gene reached the statistically significant threshold for all the remaining six traits. Because of the relatively small sample size analyzed, we alternatively selected 20 candidate genes with the lowest p values for every trait and subjected them to functional enrichment analyses, which identified three Gene Ontology (GO) terms that were significantly enriched by the candidate genes of CW and RLW traits. In conclusion, this study used TWAS approach to successfully reveal several candidate genes whose mRNA expression levels may be involved in regulating carcass and meat traits in meat rabbits, which are helpful to explore the underlying molecular mechanism in the future studies.
Serum symmetric dimethylarginine concentrations in enalapril- or telmisartan-treated dogs with proteinuric chronic kidney disease
Authors: Nasr Amirah , Lourenço Bianca N. , Coleman Amanda E. , Bartges Joseph W.
Published: December 2024

Introduction

Renin-angiotensin-aldosterone system inhibition (RAASi) reduces intraglomerular pressure and is a standard therapy for dogs with proteinuric chronic kidney disease (CKD). RAASi can acutely decrease glomerular filtration rate (GFR); however, its effects on the marker of GFR serum symmetric dimethylarginine (SDMA) concentration in dogs have not been specifically evaluated. The objective of this study was to evaluate changes, relative to pretreatment values, in serum SDMA concentrations in dogs with proteinuric CKD receiving RAASi therapy.

Methods

This retrospective study used banked samples from 29 dogs with proteinuric CKD treated with enalapril (0.5 mg/kg PO q12h; n = 16) or telmisartan (1 mg/kg PO q24h; n = 13) alone (n = 22) or in combination with amlodipine if severely hypertensive (n = 7). Serum SDMA, creatinine, and urea nitrogen (SUN) concentrations were measured before and 7 and 30 days after starting RAASi. Percentage and absolute changes in these biomarkers were calculated for each dog and time point. A linear mixed model was used to test whether changes significantly differed from zero (α < 0.05).

Results

Overall, mean ± SEM Day 7 and 30 percentage change in SDMA were  − 4.8 ± 3.6% and  − 3.2 ± 3.4%, respectively; in creatinine were 7.4 ± 3.3% and 3.0 ± 3.1%, respectively; and in SUN were 22.1 ± 6.8% and 16.7 ± 6.2%, respectively. Mean changes varied according to whether all dogs, those on RAASi alone, or those co-treated with amlodipine were evaluated. In dogs receiving RAASi alone, at day 7, there were significant mean percentual increases in creatinine (9%; p = 0.023) and SUN (23%; p = 0.005), but SDMA was unchanged. In dogs co-treated with amlodipine, a significant absolute decrease in mean SDMA (−2.29 μg/dL; p = 0.026) occurred at days 7 and 30, while mean creatinine was unchanged and mean SUN increased.

Discussion

Proteinuric dogs receiving RAASi had low-magnitude changes in serum SDMA and creatinine, and moderate-magnitude changes in SUN concentrations. The direction of change in SDMA did not consistently match that of creatinine and SUN.
Crushing Force Prediction Method of Controlled-Release Fertilizer Based on Particle Phenotype
Authors: Sun Linlin , Chen Xiubo , Chen Zixu , Jing Linlong , Wang Jinxing , Cao Xinpeng , Fu Shenghui , Jiang Yuanmao , Zhang Hongjian
Published: December 2024

This study proposed a method to predict the crushing force of controlled-release fertilizer granules based on their phenotypic characteristics to prevent coating damage during production, transport, and fertilization, which could affect nutrient diffusion rates. The phenotypic features, including sphericity, particle size, and texture, of three commonly used controlled-release fertilizers were obtained using machine vision, while the crushing force was measured using a universal testing machine. A principal component analysis was applied for data reduction, and the optimal parameters for the support vector machine (SVM) were selected using particle swarm optimization (PSO) combined with k-fold cross-validation. A particle swarm optimization–support vector machine (PSO-SVM) model was then developed to predict the crushing force based on fertilizer shape features. Compared with the traditional method, the innovation of this paper is that a non-destructive prediction method is proposed, which enables high-precision predictions of the crushing force by integrating multi-dimensional phenotypic features and an intelligent optimization algorithm. Comparative tests with a random forest regression, the K-nearest neighbor, a back propagation (BP) neural network, and a long short-term memory (LSTM) neural network have demonstrated that the PSO-SVM model outperforms these methods in terms of mean absolute error, root mean square error, and correlation coefficient, underscoring its effectiveness. The proportion of predictions within the −10% to +10% error range reached 0.82, 0.82, and 0.86 for the three fertilizers, confirming the high reliability and accuracy of the PSO-SVM method for non-destructive testing.
Structural Characteristics and Adsorption of Phosphorus by Pineapple Leaf Biochar at Different Pyrolysis Temperatures
Authors: Song Shuhui , Liu Siru , Liu Yanan , Shi Weiqi , Ma Haiyang
Published: December 2024

Biochar is a potential material for making slow-releasing phosphorus (P) fertilizers for the sake of increasing soil P-use efficiency. The adsorption of phosphorus by pineapple leaf biochar (PB) prepared at different pyrolysis temperatures and its mechanism remain unclear. In order to study the effect of preparation temperature on the structural characteristics of biochar from pineapple leaves and the adsorption of phosphorus by biochar, pineapple leaves were used as raw materials to prepare biochar by restricting oxygen supply at 300 °C, 500 °C, and 700 °C. The structural characteristics and adsorption of phosphorus by pineapple leaf biochar at different temperatures (PB300, PB500, and PB700) were analyzed. The results showed the following: (1) The pore structure of biochar pyrolysis at 300 °C (PB300) did not significantly change, while the surface structure of biochar pyrolysis at 700 °C (PB700) significantly changed, the specific surface area (SBET) increased by 26.91~37.10 times that observed in PB300 and PB500, and the pore wall became thinner. (2) The number of functional groups (C=O) in PB700 decreased, and the relative content of C-H/-CHO in PB500 and PB700 increased by 4.38 times that observed in PB300. (3) The adsorption of phosphorus by biochar was a multi-molecular layer chemisorption, accompanied by single-molecular-layer physical adsorption and intramolecular diffusion. For PB300, both the physical and chemical processes of the adsorption of PO43− by biochar were weakened, and the chemical process was dominated by cationic (Ca2+, Mg2+, Fe3+, and Al3+) adsorption at 500 °C. For PB700, the physical adsorption dominated by pore size structure was the main process, and the physicochemical adsorption at 700 °C was significantly stronger than that observed at 300 °C and 500 °C. These results indicate that biochar prepared at 500 °C can save energy in the preparation process and has excellent physical and chemical structure, which can be used as the basic material for further modification and preparation of biochar phosphate fertilizer.
Non-aureus Staphylococci Cause the Spontaneous Cure or Persistent Infection of Major Bovine Mastitis Pathogens in the Murine Mammary Glands
Authors: Suriyasathaporn Witaya , Kongkaew Aphisek , Intanon Montira , Srithanasuwan Anyaphat , Saipinta Duanghathai , Pangprasit Noppason , Thongtharb Atigan , Chuasakhonwilai Areerat , Chaisri Wasana
Published: December 2024

The microbial ecology in mastitis involves the interactions between bacteria and the mammary gland environment. Poor mastitis control, for which understanding these microbial relationships is crucial, increases the risk of mastitis and co-infections. The aim of this study was to determine the pathogenesis and bacterial ecology of murine mammary glands following intramammary infection (IMI) with S. aureus (AU), S. agalactiae (SA), and four isolates of selected non-aureus staphylococci (NAS), as well as co-infections of AU or SA with NAS. Selected NAS demonstrated resistance to growth inhibition after co-culture with AU and SA and were proven to be protective in vitro against AU and SA. Both single infections and co-infections of AU, SA, two selected isolates of S. hominis (NAS1 and NAS2), and two selected isolates of S. chromogens (NAS3 and NAS4) were injected into the murine mammary glands at 105 CFU/mL. After 72 h of inoculation, the results showed that AU, AU–NAS2, and AU–NAS3 expressed severe inflammation with clinical signs of mastitis. The persistence of AU was found for AU, NAS3, and AU–NAS3, whereas the persistence of SA was found for SA–NAS1, SA–NAS3, and SA–NAS, although single SA could not persist. Interestingly, NAS2 in both the single- and co-IMIs with AU or SA resulted in a spontaneous cure 72 h after the IMI. In conclusion, some NAS have beneficial results because they can eliminate S. aureus and S. agalactiae, but some NAS have negative effects because they can support persistent S. agalactiae infection. These results may be used to explain both the advantages and disadvantages of NAS on farms with poor mastitis control. In addition, the beneficial S. hominis may initiate natural live antibiotics and reduce antibiotic resistance problems.
Temporal Changes Toward Cellular Senescence in Rat Dental Pulp Stem Cells Induced by Long-Term In Vitro Culture
Authors: Zheng Shanshan , Nakagawa Masato , Gong Yanan , Matsushima Yasuhiko , Sasayama Satoshi , Baba Shunsuke , Honda Yoshitomo
Published: December 2024

Rat dental pulp stem cells (DPSCs) can be used to elucidate mesenchymal stem cell (MSC) applications in regenerative medicine. However, information on rat DPSCs during long-term passage, which could lead to replicative senescence, is limited. In this study, we investigated the phenotypic changes in DPSCs after 3–26 passages (3P–26P). The results show that cell morphology and nuclear size increase proportionally with passage number. The phosphorylated histone H2A.X (γ-H2A.X) positive cells (indicating DNA damage) increased significantly earlier than the 4-Hydroxynonenal (4-HNE) stained cells (indicating an abundance of intracellular reactive oxygen species). Compared to the cells subjected to 3P and 5P, the cells subjected to 15P showed reduced proliferation despite being positive for Ki67. Furthermore, cell growth was completely arrested after 26P. The senescence markers, senescence-associated β-galactosidase (SA-β-gal) and p16, exhibited similar expression patterns that were not correlated with those of p21 and urokinase-type plasminogen activator receptor (uPAR). Nearly all cells expressed SA-β-gal and p16 after 26P, whereas only half expressed p21 and uPAR. These results will contribute to understanding the characteristics of DPSCs toward replicative senescence, which are applicable to elucidate mechanisms related to regenerative medicine and stem cell aging.
Enhancing Brioche Bread with Emulsified Seed and Nut Oils: Nutritional and Sustainable Benefits
Authors: Martínez Elena , Martínez-Navarro María Esther , Pardo José E. , Rabadan Adrian , Álvarez-Ortí Manuel
Published: December 2024

This study evaluates the effectiveness of an oil-in-water emulsion formulated from water and seed and nut oils as a substitute for butter in the formulation of brioche bread. First, the selected oils were physicochemically characterized. In the brioche, animal fat was fully and partially replaced, and the effects were analyzed at the physical, chemical, and sensory levels. The new formulations exhibited increased lightness, a softer crumb, improved cohesiveness, and greater expansion compared to the control, resulting in fluffier and lighter brioches. Nutritionally, the seed and nut oil brioches showed a lower fat content and lower energy value, but higher protein and carbohydrate levels. The lipid profile was enhanced, with a higher proportion of unsaturated fatty acids, which positively impacted two heart-friendly indices and increased the vitamin E content. This improvement potentially allows the food industry to apply health claims to product labeling. Regarding sensory analysis, in all cases, the reformulated brioches scored higher than the control sample in terms of external appearance, although there remained a preference for the flavor and aroma of the traditional version, which could be mitigated by adding flavor compounds such as diacetyl. This study differs from previous research by using a seed and nut oil emulsion instead of non-emulsified oils, improving the texture and stability of brioche bread, an achievement not consistently reported in prior studies. Additionally, it emphasizes sustainability by offering a plant-based alternative that addresses the growing consumer demand for vegan and eco-friendly products while providing enhanced nutritional benefits that support potential health claims.