bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Different Exercise Types on Chrna7 and Chrfam7a Expression in Healthy Normal Weight and Overweight Type 2 Diabetic Adults
Authors: Mateus Keryma Chaves da Silva , Bonfante Ivan Luiz Padilha , Sardeli Amanda Veiga , Duft Renata Garbellini , Gáspari Arthur Fernandes , Trombeta Joice Cristina dos Santos , Morari Joseane , Rodrigues Bruno , Torsoni Márcio Alberto , Chacon-Mikahil Mara Patrícia Traina , Velloso Licio Augusto , Cavaglieri Cláudia Regina
Published: February 2023

Purpose: Considering that the CHRNA7 and CHRFAM7A genes can be modulated by acute or chronic inflammation, and exercise modulates inflammatory responses, the question that arises is whether physical exercise could exert any effect on the expression of these genes. Thus, the aim of this work is to identify the effects of different types of exercises on the expression of the CHRNA7, CHRFAM7A and tumor necrosis factor-α (TNF-α) in leukocytes of healthy normal weight (HNW), and overweight with type 2 diabetes (OT2D) individuals. Methods: 15 OT2D and 13 HNW participants (men and women, from 40 to 60 years old) performed in a randomized crossover design three exercise sessions: aerobic exercise (AE), resistance exercise (RE) and combined exercise (CE). Blood samples were collected at rest and post-60-min of the exercise sessions. The leukocytes were the analysis of the CHRNA7, CHRFAM7A and (TNF-α) gene expression. Results: At baseline, OT2D had higher CHRFAM7A and TNF-α expression compared to HNW. No statistical differences were observed between groups for CHRNA7; however, the HNW group presented almost twice as many subjects with the expression of this gene (24% vs. 49%). Post exercise, the CHRFAM7A increased in AE, RE and CE for HNW, and in AE and CE for OT2D. There was no significant difference for TNF-α and CHRNA7 expression between any type of exercise and group. Conclusions: Our study shows that OT2D individuals presented higher baseline expression of TNF-α and CHRFAM7A, besides evidence of decreased CHRNA7A expression in leukocytes when compared with HNW. On the other hand, acutely physical exercise induces increased CHRFAM7A expression, especially when the aerobic component is present.
Urate Transporter 1 Can Be a Therapeutic Target Molecule for Chronic Kidney Disease and Diabetic Kidney Disease: A Retrospective Longitudinal Study
Authors: Yanai Hidekatsu , Katsuyama Hisayuki , Hakoshima Mariko , Adachi Hiroki
Published: February 2023

Chronic kidney disease (CKD) is a major global health problem for which there are no curative drug treatments. Hyperuricemia is one of risk factors for CKD. The evidence on effects of uric acid (UA)-lowering treatments on the progression of CKD was very limited and previous meta-analyses used only trials which primarily used xanthin oxidase (XO) inhibitors because the reports on fulminant hepatitis due to benzbromarone kept us from using uricosuric agents for hyperuricemia patients. Dotinurad, a novel selective urate reabsorption inhibitor for the treatment of hyperuricemia, reduces serum UA levels by selectively inhibiting urate transporter 1 (URAT1). We retrospectively picked up patients who had taken dotinurad from June 2018 to August 2021 and compared metabolic parameters at baseline with the data at 3 and 6 months after the start of dotinurad. We found 84 patients, and approximately 74% of patients were complicated with CKD. After the start of dotinurad, improvements in serum lipids, systolic blood pressure, body weight, and albuminuria, in addition to reduction in serum UA, were observed. Dotinurad increased urinary UA excretion, and was effective to reduce serum UA in patients with both UA underexcretion type and renal UA overload type. Furthermore, urinary UA excretion was significantly and negatively correlated with serum creatine levels at baseline and at 6 months after the start of dotinurad, and the change in urinary UA excretion after 3 months was significantly and negatively correlated with change in serum creatine levels. The property of dotinurad, which selectively inhibits URAT1, but not other UA transporters, such as ATP-binding cassette, subfamily G, and 2 (ABCG2), which ABCG2 is a UA and uremic toxin exporter, may be beneficially associated with pathology of CKD. URAT1 can be a therapeutic target molecule for CKD and DKD.
Bacterial Outer Membrane Vesicles Promote Lung Inflammatory Responses and Macrophage Activation via Multi-Signaling Pathways
Authors: Ryu Sunhyo , Ni Kareemah , Wang Chenghao , Sivanantham Ayyanar , Carnino Jonathan M. , Ji Hong-Long , Jin Yang
Published: February 2023

Emerging evidence suggests that Gram-negative bacteria release bacterial outer membrane vesicles (OMVs) and that these play an important role in the pathogenesis of bacterial infection-mediated inflammatory responses and organ damage. Despite the fact that scattered reports have shown that OMVs released from Gram-negative bacteria may function via the TLR2/4-signaling pathway or induce pyroptosis in macrophages, our study reveals a more complex role of OMVs in the development of inflammatory lung responses and macrophage pro-inflammatory activation. We first confirmed that various types of Gram-negative bacteria release similar OMVs which prompt pro-inflammatory activation in both bone marrow-derived macrophages and lung alveolar macrophages. We further demonstrated that mice treated with OMVs via intratracheal instillation developed significant inflammatory lung responses. Using mouse inflammation and autoimmune arrays, we identified multiple altered cytokine/chemokines in both bone marrow-derived macrophages and alveolar macrophages, suggesting that OMVs have a broader spectrum of function compared to LPS. Using TLR4 knock-out cells, we found that OMVs exert more robust effects on activating macrophages compared to LPS. We next examined multiple signaling pathways, including not only cell surface antigens, but also intracellular receptors. Our results confirmed that bacterial OMVs trigger both surface protein-mediated signaling and intracellular signaling pathways, such as the S100-A8 protein-mediated pathway. In summary, our studies confirm that bacterial OMVs strongly induced macrophage pro-inflammatory activation and inflammatory lung responses via multi-signaling pathways. Bacterial OMVs should be viewed as a repertoire of pathogen-associated molecular patterns (PAMPs), exerting more robust effects than Gram-negative bacteria-derived LPS.
Novel Therapeutic Targets for Migraine
Authors: Nisar Areeba , Ahmed Zubair , Yuan Hsiangkuo
Published: February 2023

Migraine, a primary headache disorder involving a dysfunctional trigeminal vascular system, remains a major debilitating neurological condition impacting many patients’ quality of life. Despite the success of multiple new migraine therapies, not all patients achieve significant clinical benefits. The success of CGRP pathway-targeted therapy highlights the importance of translating the mechanistic understanding toward effective therapy. Ongoing research has identified multiple potential mechanisms in migraine signaling and nociception. In this narrative review, we discuss several potential emerging therapeutic targets, including pituitary adenylate cyclase-activating polypeptide (PACAP), adenosine, δ-opioid receptor (DOR), potassium channels, transient receptor potential ion channels (TRP), and acid-sensing ion channels (ASIC). A better understanding of these mechanisms facilitates the discovery of novel therapeutic targets and provides more treatment options for improved clinical care.
Evaluation of Resin Infiltration, Fluoride and the Biomimetic Mineralization of CPP-ACP in Protecting Enamel after Orthodontic Inter-Proximal Enamel Reduction
Authors: Almansouri Naser , Bakry Ahmed Samir , Abbassy Mona Aly , Linjawi Amal Ibrahim , Hassan Ali Habib
Published: February 2023

Background: This study investigated the effect of using different agents for protecting enamel proximal surfaces against acidic attack after interproximal reduction (IPR) using the trans micro radiography technique. Methods: Seventy-five sound-proximal surfaces were obtained from extracted premolars for orthodontic reasons. All teeth were measured miso-distally and mounted before being stripped. The proximal surfaces of all teeth were hand stripped with single-sided diamond strips (OrthoTechnology, West Columbia, SC, USA) followed by polishing via Sof-Lex polishing strips (3M, Maplewood, MN, USA). Three-hundred micrometers of enamel thickness was reduced from each proximal surface. The teeth were randomly divided into 5 groups: group 1 (control un-demineralized) received no treatment, group 2 (control demineralized) had their surfaces demineralized after the IPR procedure, group 3 (fluoride) specimens were treated with fluoride gel (NUPRO, DENTSPLY, Charlotte, NC, USA) after the IPR, group 4 (Icon) resin infiltration material (Icon Proximal Mini Kit, DMG, Bielefeld, Germany) was applied after IPR, group 5 (MI varnish) specimens were treated with Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) containing varnish (MI Varnish, G.C, USA, St. Alsip, IL, USA) after the IPR. The specimens in (groups 2–5) were stored in a 4.5 pH demineralization solution for 4 days. The trans-micro-radiography (TMR) technique was conducted to evaluate the mineral loss (∆Z) and lesion depth of all specimens after the acid challenge. The obtained results were analyzed statistically using a one-way ANOVA at a significance level of α = 0.05. Results: The MI varnish recorded significant ∆Z and lesion depth values compared to the other groups p > 0.05. There was no significant difference in ∆Z and lesion depth between the control demineralized, Icon, and fluoride groups p < 0.05. Conclusion: The MI varnish increased the enamel resistance to acidic attack, and thus can be considered an agent capable of protecting the proximal enamel surface after IPR.
Arc-Mediated Synaptic Plasticity Regulates Cognitive Function in a Migraine Mouse Model
Authors: Hu Luyu , Gong Qiaoyu , Zhou Yanjie , Wang Yajuan , Qiu Tao , Fang Yuting , Huang Wanbin , Liang Jingjing , Xiao Zheman
Published: February 2023

Previous clinical and basic studies have shown that migraine is associated with cognitive impairment, anxiety, and depression. It severely affects the quality of life. In this study, C57BL/6 mice were randomly divided into four groups: IS group, IS+M group, and IS+S group with repeated application of dural inflammatory soup (IS) stimulation to establish a migraine model, followed by PBS, memantine, and sumatriptan interventions, respectively; the blank control group underwent the same treatment procedure but with PBS instead of IS and intervention drugs. The cognitive function of the mice was used as the main outcome indicator. After application of the IS, mice showed reduced pain threshold for mechanical stimulation, decreased learning memory capacity, attention deficit, a reduced number of dendritic spines in hippocampal neurons, and altered synaptic ultrastructure. The cognitive function indexes of mice in the IS+M group recovered with changes in Arc protein expression to a level not statistically different from that of the Control group, while the IS and IS+S groups remained at lower levels. The present results suggest that Arc-mediated synaptic plasticity may be an essential mechanism of cognitive dysfunction in migraine.
Joule Heating of Carbon-Based Materials Obtained by Carbonization of Para-Aramid Fabrics
Authors: Karthik Daniel , Militky Jiri , Wang Yuanfeng , Venkataraman Mohanapriya
Published: February 2023

The Joule heating behavior of carbon-based materials obtained by the process of carbonization of industrial para-aramid fabric wastes are investigated in the present work. Carbonization involves a process of thermally decomposing organic material, thereby altering its physical and chemical properties to obtain carbon-rich materials that are electrically conductive and display Joule heating behavior. The principle of Joule heating is based on the intrinsic electrical resistance of the material across an applied voltage. Here, para-aramid woven fabric wastes are converted into activated carbon materials through straightforward, controlled, single-step thermal treatments by three different kinds of atmosphere, i.e., in the CO2 evolved from charcoal, a mixture of gases from ammonium bicarbonate salt (NH4HCO3), and Nitrogen gas (N2), respectively, inside a high-temperature furnace. The carbonization temperatures were varied from 800 to 1100 °C. The carbonization process variables were optimized to obtain carbon-rich materials with lower electrical resistivity. The results of electrical resistivity measurements show that for all three methods, the electrical resistivity decreases with increasing carbonization temperatures. An experimental setup consisting of an infrared (IR) camera, positioned over the surface of the fabric specimen to record the surface temperature of the material connected to a DC power supply, was employed. The kinetics of Joule heating and subsequent cooling were also analyzed at a fixed voltage of 5 V by recording the changes in surface temperature with respect to time. The heating–cooling cycle is described by a simple kinetic model of first order.
Does the Immunohistochemical Expression of CD44, MMP-2, and MMP-9 in Association with the Histopathological Subtype of Renal Cell Carcinoma Affect the Survival of Patients with Renal Cancer?
Authors: Chrabańska Magdalena , Rynkiewicz Magdalena , Kiczmer Paweł , Drozdzowska Bogna
Published: February 2023

CD44, MMP-2, and MMP-9 are new potential molecular prognostic markers in renal cell carcinoma (RCC). The aim of the study was to analyze whether the expression of CD44, MMP-2, and MMP-9 in association with the histopathological subtype of RCC affects the survival of patients with renal cancer. The study population included 243 clear cell RCC (ccRCC) and 59 non-ccRCC cases. A total of 302 tumors were examined for CD44, MMP2, and MMP9 expression by immunohistochemistry. The expression levels of the proteins were scored by semi-quantitative methods, and the correlation with overall patient survival was verified. We found no significant differences in CD44 expression levels between cc-RCC and non-ccRCC cases; however, significant differences existed in the degree of MMP-2 and MMP-9 expression between cc-RCC and non-ccRCC cases. There was significantly higher MMP expression in non-ccRCC than in ccRCC cases. Univariate Cox regression analysis showed that increased CD44 expression and histopathological subtype of ccRCC were predictors of shorter overall survival. Moreover, MMP-2 overexpression slightly reduced the risk of patient death, while MMP-9 expression did not show an association with patients’ survival. However, on multivariate analysis, only the histopathological subtypes of ccRCC and CD44 expression were independent risk factors for patient death.
Association between Tumor Mutational Burden, Stromal CD8+ Tumor-Infiltrating Lymphocytes, and Clinical Factors in Cervical Cancers Treated with Radiotherapy
Authors: Ruan Hanguang , Oike Takahiro , Sato Hiro , Ando Ken , Ohno Tatsuya
Published: February 2023

Background: Tumor mutational burden (TMB) and stromal CD8-positive tumor-infiltrating lymphocytes (CD8+TILs) serve important roles in antitumor immune responses to radiotherapy. This study aimed to elucidate the association between TMB, CD8+TILs, and clinical factors in patients with cervical cancer treated with radiotherapy. Methods: Patients with squamous cell carcinoma of the uterine cervix treated with definitive radiotherapy, and with available somatic mutation data and immunohistochemical staining data from identical tumor tissues, were enrolled retrospectively. The association between TMB and/or CD8+TIL density and patient characteristics, mutation profiles, and treatment outcome was analyzed. Results: The study analyzed 44 patients (median follow-up period, 61 months). There was no significant correlation between TMB and CD8+TIL density, or between TMB or CD8+TIL density and patient characteristics. TMB-high or CD8+TIL density-low status was associated with worse overall survival and distant metastasis-free survival; the predictive value of these factors became greater when used in combination. TMB-high or CD8+TIL density-high status was associated with ARID1A mutations. Conclusions: These data indicate independence of TMB and CD8+TIL density and the involvement of ARID1A alterations in antitumor immune responses in patients with cervical cancers treated with radiotherapy, warranting further mechanistic research and prospective validation.
Soft Tissue Reconstruction of the Posterior Trunk after Tumor Excision: A Surgical Algorithm
Authors: Innocenti Marco , Mori Francesco , Pedrini Francesca Alice , Salmaso Luca , Gennaro Andrea , Sassu Paolo
Published: February 2023

Background: The posterior trunk has been considered a challenging area to reconstruct following soft tissue tumor excision because of the shortage of local donor sites. The advent of innovative procedures such as perforator flaps has radically changed this perspective and offered a new approach to the problem. Methods: Upon a review of the literature and the personal experiences of the senior author, an algorithm is developed according to the most updated procedure, combined with more conventional options that maintain a role in decision-making. Results: The upper back latissimus dorsi and trapezium flap are still the most reliable approaches, while perforator flaps based either on the circumflex scapular arteries or the transverse cervical artery represent a more refined option. In the middle third, few indications remain for the reverse latissimus dorsi, while the gold standard is represented by local perforator flaps based on the posterior intercostal system. In the lower back, conventional VY advancement flaps are still a safe and effective option in the sacral area, and perforator flaps based on posterior intercostal arteries, lumbar arteries and superior gluteal arteries are the first choice in most cases. Conclusions: Using perforator flaps significantly improved soft tissue reconstruction in the posterior trunk.
Decoupling of mRNA and Protein Expression in Aging Brains Reveals the Age-Dependent Adaptation of Specific Gene Subsets
Authors: Khatir Inès , Brunet Marie A. , Meller Anna , Amiot Florent , Patel Tushar , Lapointe Xavier , Avila Lopez Jessica , Guilloy Noé , Castonguay Anne , Husain Mohammed Amir , Germain Joannie St. , Boisvert François-Michel , Plourde Mélanie , Roucou Xavier , Laurent Benoit
Published: February 2023

During aging, changes in gene expression are associated with a decline in physical and cognitive abilities. Here, we investigate the connection between changes in mRNA and protein expression in the brain by comparing the transcriptome and proteome of the mouse cortex during aging. Our transcriptomic analysis revealed that aging mainly triggers gene activation in the cortex. We showed that an increase in mRNA expression correlates with protein expression, specifically in the anterior cingulate cortex, where we also observed an increase in cortical thickness during aging. Genes exhibiting an aging-dependent increase of mRNA and protein levels are involved in sensory perception and immune functions. Our proteomic analysis also identified changes in protein abundance in the aging cortex and highlighted a subset of proteins that were differentially enriched but exhibited stable mRNA levels during aging, implying the contribution of aging-related post- transcriptional and post-translational mechanisms. These specific genes were associated with general biological processes such as translation, ribosome assembly and protein degradation, and also important brain functions related to neuroplasticity. By decoupling mRNA and protein expression, we have thus characterized distinct subsets of genes that differentially adjust to cellular aging in the cerebral cortex.
Complementary Cell Lines for Protease Gene-Deleted Single-Cycle Adenovirus Vectors
Authors: Elahi Seyyed Mehdy , Nazemi-Moghaddam Nazila , Guilbault Claire , Simoneau Mélanie , Gilbert Rénald
Published: February 2023

To increase the safety of adenovirus vector (AdV)-based therapy without reducing its efficacy, a single-cycle adenovirus vector (SC-AdV) with a deletion in the protease gene (PS) was developed in order to be used as a substitute for the replication-competent adenovirus (RC-AdV). Since no infectious viral particles are assembled, there is no risk of viral shedding. The complementary cell lines for this developed AdV proved to be suboptimal for the production of viral particles and require the presence of fetal bovine serum (FBS) to grow. In the current study, we produced both stable pools and clones using adherent and suspension cells expressing the PS gene. The best adherent cell pool can be used in the early stages for the generation of protease-deleted adenovirus, plaque purification, and titration. Using this, we produced over 3400 infectious viral particles per cell. Additionally, the best suspension subclone that was cultured in the absence of FBS yielded over 4000 infectious viral particles per cell. Harvesting time, culture media, and concentration of the inducer for the best suspension subclone were further characterized. With these two types of stable cells (pool and subclone), we successfully improved the titer of protease-deleted adenovirus in adherent and suspension cultures and eliminated the need for FBS during the scale-up production. Eight lots of SC-AdV were produced in the best suspension subclone at a scale of 2 to 8.2 L. The viral and infectious particle titers were influenced by the virus backbone and expressed transgene.
Role of Astrocytes in Parkinson’s Disease Associated with Genetic Mutations and Neurotoxicants
Authors: Kim Sanghoon , Pajarillo Edward , Nyarko-Danquah Ivan , Aschner Michael , Lee Eunsook
Published: February 2023

Parkinson’s disease (PD) is a neurodegenerative disorder characterized by the loss of dopaminergic neurons and the aggregation of Lewy bodies in the basal ganglia, resulting in movement impairment referred to as parkinsonism. However, the etiology of PD is not well known, with genetic factors accounting only for 10–15% of all PD cases. The pathogenetic mechanism of PD is not completely understood, although several mechanisms, such as oxidative stress and inflammation, have been suggested. Understanding the mechanisms of PD pathogenesis is critical for developing highly efficacious therapeutics. In the PD brain, dopaminergic neurons degenerate mainly in the basal ganglia, but recently emerging evidence has shown that astrocytes also significantly contribute to dopaminergic neuronal death. In this review, we discuss the role of astrocytes in PD pathogenesis due to mutations in α-synuclein (PARK1), DJ-1 (PARK7), parkin (PARK2), leucine-rich repeat kinase 2 (LRRK2, PARK8), and PTEN-induced kinase 1 (PINK1, PARK6). We also discuss PD experimental models using neurotoxins, such as paraquat, rotenone, 6-hydroxydopamine, and MPTP/MPP+. A more precise and comprehensive understanding of astrocytes’ modulatory roles in dopaminergic neurodegeneration in PD will help develop novel strategies for effective PD therapeutics.
Lobule-Related Action Potential Shape- and History-Dependent Current Integration in Purkinje Cells of Adult and Developing Mice
Authors: Beekhof Gerrit C. , Schonewille Martijn
Published: February 2023

Purkinje cells (PCs) are the principal cells of the cerebellar cortex and form a central element in the modular organization of the cerebellum. Differentiation of PCs based on gene expression profiles revealed two subpopulations with distinct connectivity, action potential firing and learning-induced activity changes. However, which basal cell physiological features underlie the differences between these subpopulations and to what extent they integrate input differentially remains largely unclear. Here, we investigate the cellular electrophysiological properties of PC subpopulation in adult and juvenile mice. We found that multiple fundamental cell physiological properties, including membrane resistance and various aspects of the action potential shape, differ between PCs from anterior and nodular lobules. Moreover, the two PC subpopulations also differed in the integration of negative and positive current steps as well as in size of the hyperpolarization-activated current. A comparative analysis in juvenile mice confirmed that most of these lobule-specific differences are already present at pre-weaning ages. Finally, we found that current integration in PCs is input history-dependent for both positive and negative currents, but this is not a distinctive feature between anterior and nodular PCs. Our results support the concept of a fundamental differentiation of PCs subpopulations in terms of cell physiological properties and current integration, yet reveals that history-dependent input processing is consistent across PC subtypes.
Lewy Body-like Pathology and Loss of Dopaminergic Neurons in Midbrain Organoids Derived from Familial Parkinson’s Disease Patient
Authors: Becerra-Calixto Andrea , Mukherjee Abhisek , Ramirez Santiago , Sepulveda Sofia , Sinha Tirthankar , Al-Lahham Rabab , De Gregorio Nicole , Gherardelli Camila , Soto Claudio
Published: February 2023

Progressive accumulation of α-Synuclein (αSyn) in Lewy bodies (LBs) and loss of dopaminergic (DA) neurons are the hallmark pathological features of Parkinson’s disease (PD). Although currently available in vitro and in vivo models have provided crucial information about PD pathogenesis, the mechanistic link between the progressive accumulation of αSyn into LBs and the loss of DA neurons is still unclear. To address this, it is critical to model LB formation and DA neuron loss, the two key neuropathological aspects of PD, in a relevant in vitro system. In this study, we developed a human midbrain-like organoid (hMBO) model of PD. We demonstrated that hMBOs generated from induced pluripotent stem cells (hiPSCs), derived from a familial PD (fPD) patient carrying αSyn gene (SNCA) triplication accumulate pathological αSyn over time. These cytoplasmic inclusions spatially and morphologically resembled diverse stages of LB formation and were composed of key markers of LBs. Importantly, the progressive accumulation of pathological αSyn was paralleled by the loss of DA neurons and elevated apoptosis. The model developed in this study will complement the existing in vitro models of PD and will provide a unique platform to study the spatiotemporal events governing LB formation and their relation with neurodegeneration. Furthermore, this model will also be beneficial for in vitro screening and the development of therapeutic compounds.
Expression of Basement Membrane Molecules by Wharton Jelly Stem Cells (WJSC) in Full-Term Human Umbilical Cords, Cell Cultures and Microtissues
Authors: Sánchez-Porras David , Durand-Herrera Daniel , Carmona Ramón , Blanco-Elices Cristina , Garzón Ingrid , Pozzobon Michela , San Martín Sebastián , Alaminos Miguel , García-García Óscar Darío , Chato-Astrain Jesús , Carriel Víctor
Published: February 2023

Wharton’s jelly stem cells (WJSC) from the human umbilical cord (UC) are one of the most promising mesenchymal stem cells (MSC) in tissue engineering (TE) and advanced therapies. The cell niche is a key element for both, MSC and fully differentiated tissues, to preserve their unique features. The basement membrane (BM) is an essential structure during embryonic development and in adult tissues. Epithelial BMs are well-known, but similar structures are present in other histological structures, such as in peripheral nerve fibers, myocytes or chondrocytes. Previous studies suggest the expression of some BM molecules within the Wharton’s Jelly (WJ) of UC, but the distribution pattern and full expression profile of these molecules have not been yet elucidated. In this sense, the aim of this histological study was to evaluate the expression of main BM molecules within the WJ, cultured WJSC and during WJSC microtissue (WJSC-MT) formation process. Results confirmed the presence of a pericellular matrix composed by the main BM molecules—collagens (IV, VII), HSPG2, agrin, laminin and nidogen—around the WJSC within UC. Additionally, ex vivo studies demonstrated the synthesis of these BM molecules, except agrin, especially during WJSC-MT formation process. The WJSC capability to synthesize main BM molecules could offer new alternatives for the generation of biomimetic-engineered substitutes where these molecules are particularly needed.
Formaldehyde Gas Sensing Characteristics of ZnO-TiO2 Gas Sensors
Authors: Park Jaebum , Lee Jihoon , Choi Myung Sik , Huh Jeung-Soo
Published: February 2023

Since the increase in the emission of various Volatiles Organic Compounds, gas and formaldehyde gas have had a harmful effect on the human body, and gas sensors that can measure those gases were fabricated in this study. After Pt coating was performed on the alumina substrate, Zn seed layers were fabricated. Nanostructures were formed through sonochemical synthesis by varying the ratio of ZnO and TiO2. Thereafter, the reactivity and recovery properties were compared and evaluated according to the concentrations of formaldehyde and toluene gas. The ZnO(99%)-TiO2(1%) gas sensor showed meaningful selectivity of about 40% or more at a concentration ranging from 5 to 20 ppm (high concentration) of formaldehyde and toluene gas, and showed a low selectivity of about 5% or more for a concentration ranging from 0.1 to 1 ppm (low concentration) of formaldehyde and toluene gas. This sensor can be optimized to have a meaningful selectivity of formaldehyde gas compared to other Volatiles Organic Compounds gases by optimizing the ZnO-TiO2 nanostructure.
Back Pain without Disease or Substantial Injury in Children and Adolescents: A Twin Family Study Investigating Genetic Influence and Associations
Authors: Beerstra Tessa , Bui Minh , Jaaniste Tiina , Bott Aneeka , Hopper John , Champion G.
Published: February 2023

This twin family study first aimed to investigate the evidence for genetic factors predicting the risk of lifetime prevalence of non-specific low back pain of at least three months duration (LBP (life)) and one-month current prevalence of thoracolumbar back pain (TLBP (current)) using a study of children, adolescents, and their first-degree relatives. Secondly, the study aimed to identify associations between pain in the back with pain in other regions and also with other conditions of interest. Randomly selected families (n = 2479) with child or adolescent twin pairs and their biological parents and first siblings were approached by Twins Research Australia. There were 651 complete twin pairs aged 6–20 years (response 26%). Casewise concordance, correlation, and odds ratios were compared for monozygous (MZ) and dizygous (DZ) pairs to enable inference about the potential existence of genetic vulnerability. Multivariable random effects logistic regression was used to estimate associations between LBP (life) or TLBP (current) as an outcome with the potentially relevant condition as predictors. The MZ pairs were more similar than the DZ pairs for each of the back pain conditions (all p values < 0.02). Both back pain conditions were associated with pain in multiple sites and with primary pain and other conditions using the combined twin and sibling sample (n = 1382). Data were consistent with the existence of genetic influences on the pain measures under the equal environments assumption of the classic twin model and associations with both categories of back pain were consistent with primary pain conditions and syndromes of childhood and adolescence which has research and clinical implications.
Life Events in the Etiopathogenesis and Maintenance of Restrictive Eating Disorders in Adolescence
Authors: Baradel Giorgia , Pratile Diletta Cristina , Orlandi Marika , Vecchio Arianna , Casini Erica , De Giorgis Valentina , Borgatti Renato , Mensi Martina Maria ,   the Mondino Foundation Eating Disorders Clinical Research Group
Published: February 2023

Life events (traumatic and protective) may be critical factors associated with eating disorders and their severity. To date, there is little literature concerning the role of life events in adolescence. The main goal of this study was to explore in a sample of adolescent patients with restrictive eating disorders (REDs) the presence of life events in the year before enrolment and to characterize them according to timing. Furthermore, we investigated correlations between REDs severity and the presence of life events. In total, 33 adolescents completed the EDI-3 questionnaire to assess RED severity using EDRC (Eating Disorder Risk Composite), GPMC (General Psychological Maladjustment Composite), and the Coddington Life Events Scales—Adolescent (CLES-A) questionnaires to define the presence of life events in the last year. Of these, 87.88% reported a life event in the past year. A significant association emerged between elevated clinical GPMC and the presence of traumatic events: patients who had experienced at least one traumatic life event in the year before enrolment presented higher clinically elevated GPMC compared to patients who had not. These results suggest that obtaining early information about traumatic events in clinical practice may help prevent the occurrence of new events and improve patient outcomes.
Single-Stage Tibial Osteotomy for Correction of Genu Varum Deformity in Children
Authors: Kolbe Nikolas , Haydon Frank , Kolbe Johannes , Dreher Thomas
Published: February 2023

Conservative and operative treatments with gradual or acute correction of severe varus deformities of the leg have been described. We evaluated whether the corrective osteotomy used within the NGO Mercy Ships is an effective treatment for genu varum deformity of different etiologies in children and which patient specific factors have an influence on the radiographic outcome. In total, 208 tibial valgisation osteotomies were performed in 124 patients between 2013 and 2017. The patients’ mean age at the time of surgery was 8.4 (2.9 to 16.9 (min/max)) years. Seven radiographically measured angles were used to assess the deformity. Clinical photographs taken pre- and postoperatively were assessed. The mean time between the surgery and the end of physiotherapeutic treatment was 13.5 (7.3 to 28) weeks. Complications were monitored and classified according to the modified Clavien–Dindo-classification system. The mean preoperative mechanical tibiofemoral angle was 42.1° varus (range: 85°–12° varus). The mean postoperative mechanical tibiofemoral angle was 4.3° varus (range: 30° varus–13° valgus). The factors predicting a residual varus deformity were higher age, greater preoperative varus deformity and the diagnosis of Blount disease. The tibiofemoral angle measured on routine clinical photographs correlated well with the radiographic measurements. The single-stage tibial osteotomy described is a simple, safe and cost-effective technique to correct three-dimensional deformities of the tibia. Our study shows very good mean postoperative results, but with a higher variability than in other studies published. Nevertheless, considering the severity of preoperative deformities and the limited opportunities for aftercare, this method is excellent for the correction of varus deformities.
Body Composition, Eating Habits, and Disordered Eating Behaviors among Adolescent Classical Ballet Dancers and Controls
Authors: Chaikali Panagiota , Kontele Ioanna , Grammatikopoulou Maria G. , Oikonomou Eleftheria , Sergentanis Theodoros N. , Vassilakou Tonia
Published: February 2023

Adolescent classical ballet dancers are nutritionally vulnerable, as they try to retain a lean body shape during a life period of high nutritional requirements due to rapid growth. Studies conducted on adult dancers have indicated a high risk for the development of disordered eating behaviors (DEBs), but research on adolescent dancers remains scarce. The aim of the present case-control study was to compare the body composition, dietary habits, and DEBs of female adolescent classical ballet dancers and their non-dancer same-sex peers. Self-reported questionnaires, namely the Eating Attitudes Test-26 (EAT-26) and a 19-item Food Frequency Questionnaire (FFQ), were used for the assessment of habitual diet and DEBs. The assessment of body composition included the measurements of body weight, height, body circumference, and skinfolds and bioelectrical impedance analysis (BIA). The results indicate that the dancers were leaner than the controls, with lower weight, BMIs, and hip and arm circumferences, leaner skinfolds, and less fat mass. No differences were observed between the two groups regarding eating habits and the EAT-26 scores, but almost 1 out of 4 (23.3%) participants scored ≥ 20, indicative of DEBs. Participants with an EAT-26 score ≥ 20 had significantly higher body weight, BMIs, body circumferences, fat mass, and fat-free mass than those with a score < 20. Adolescents must be educated on nutrition and healthy methods to control body weight through evidence-based information and programs, and whenever appropriate, also through individual counseling by the appropriate health professionals.
Recent Advances in the Structural Design of Silicon/Carbon Anodes for Lithium Ion Batteries: A Review
Authors: Mei Yanan , He Yuling , Zhu Haijiang , Ma Zeyu , Pu Yi , Chen Zhilin , Li Peiwen , He Liang , Wang Wenwu , Tang Hui
Published: February 2023

As the capacity of lithium-ion batteries (LIBs) with commercial graphite anodes is gradually approaching the theoretical capacity of carbon, the development of silicon-based anodes, with higher energy density, has attracted great attention. However, the large volume variation during its lithiation/de-lithiation tends to lead to capacity decay and poor cycling performance. While rationally designed silicon/carbon (Si/C) anodes can exhibit higher specific capacity by virtue of silicon and high electrical conductivity and volume expansion suppression by virtue of carbon, they still show poor cycling performance with low initial coulombic efficiency. This review focuses on three strategies for structural design and optimization of Si/C anodes, i.e., carbon-coated structure, embedded structure and hollow structure, based on the recent researches into Si/Canodes and provides deeper insights into the problems that remain to be addressed.
An Exploratory Ecological Study between COVID-19 Vaccination Rate and Racial/Ethnic and Socioeconomic Status Neighborhood Conditions in Michigan
Authors: Argeros Grigoris , Hoffman Jenni L. , Dove Natalie
Published: February 2023

COVID-19 vaccination rate disparities continue to persist in the U.S., despite their wide availability. A multidimensional host of sociodemographic, economic, political, and cultural factors have resulted in differences in vaccine coverage rates across the U.S. The present study descriptively explores one component of the complex relationship among what drives COVID-19 vaccination rate differences—specifically, the relationship between neighborhood sociodemographic characteristics and vaccination rates in Michigan. Data from the 2019 5-Year American Community Survey are merged with vaccine coverage rate data at the census tract level for the 18-years-and-older population for Michigan from 15 December 2020 to 19 November 2021. On the one hand, the results reveal that a neighborhood’s racial and ethnic composition and socioeconomic status characteristics are part of the complex bundle of characteristics affecting neighborhood vaccination coverage rates across Michigan. On the other hand, the findings also reveal a set of surprising patterns as they relate to the racial and ethnic composition of neighborhoods in Michigan. Future studies should further explore the extent to which race/ethnicity and class-related neighborhood characteristics are statistically relevant in examining the broader community effects on vaccine coverage rates in Michigan.
Attacking Windows Hello for Business: Is It What We Were Promised?
Authors: Haddad Joseph , Pitropakis Nikolaos , Chrysoulas Christos , Lemoudden Mouad , Buchanan William J.
Published: February 2023

Traditional password authentication methods have raised many issues in the past, including insecure practices, so it comes as no surprise that the evolution of authentication should arrive in the form of password-less solutions. This research aims to explore the problems that password authentication and password policies present and aims to deploy Windows Hello for Business (WHFB) on-premises. This includes creating three virtual machines (VMs) and evaluating WHFB as a password-less solution and showing how an attacker with privileged access may retrieve the end user’s domain password from the computer’s memory using Mimikatz and describing the possible results. The conducted research tests are in the form of two attack methods. This was feasible by the creation of three VMs operating in the following way. The first VM will act as a domain controller (DC) and certificate authority server (CA server). The second VM will act as an Active Directory Federation Service (ADFS). The third VM will act as the end-user device. The test findings research summarized that password-less authentication is far more secure than the traditional authentication method; this is evidenced throughout the author’s tests. Within the first test, it was possible to retrieve the password from an enrolled device for WHFB while it was still in the second phase of the deployment. The second test was a brute-force attack on the PIN of WHFB; since WHFB has measures to prevent such attacks, the attack was unsuccessful. However, even though the retrieval of the password was successful, there are several obstacles to achieving this outcome. It was concluded that many organizations still use password authentication as their primary authentication method for accessing devices and applications. Larger organizations such as Microsoft and Google support the adoption of password-less authentication for end-users, and the current usage of password-less authentication shared by both organizations is encouraged. This usually leads organizations to adopt this new solution for their IT infrastructure. This is because it has been used and tested by millions of people and has proven to be safe. This supports the findings of increased usage and the need for password-less authentication by today’s users.
Ginzburg–Landau Analysis on the Physical Properties of the Kagome Superconductor CsV3Sb5
Authors: Han Tianyi , Che Jiantao , Ye Chenxiao , Huang Hai
Published: February 2023

The kagome lattice consisting of corner-sharing triangles has been studied in the context of quantum physics for more than seventy years. For the novel discovered kagome superconductor CsV3Sb5, identifying the pairing symmetry of order parameter remained an elusive problem until now. Based on the two-band Ginzburg–Landau theory, we study the temperature dependence of upper critical field and magnetic penetration depth for this compound. All theoretical results are consistent with the experimental data, which strongly indicates the existence of two-gap s-wave superconductivity in this system. In addition, it is worth noting that the anisotropy of effective masses in the band with large (or small) gap is about 70 (or 2.4). With the calculation of the Kadowaki–Woods ratio as 0.58×10−5μΩ cm mol2 K2 mJ−2, the semi-heavy-fermion feature is suggested in the compound CsV3Sb5.
Investigation of the Temperature Dependence of Volt-Ampere Characteristics of a Thin-Film Si3N4 Memristor
Authors: Mizginov Dmitry , Telminov Oleg , Yanovich Sergey , Zhevnenko Dmitry , Meshchaninov Fedor , Gornev Evgeny
Published: February 2023

The compatibility of memristor materials with advanced complementary metal-oxide-semiconductor (CMOS) technology is a key factor for microelectronics element base manufacturing. Therefore, we continued studying previously fabricated CMOS-compatible Ni/Si3N4/SiO2/p+-Si samples. We approximated volt-ampere characteristics (VAC) at different temperatures using the general form of the spatial charge-limiting current (SCLC) equation assuming exponential and Gaussian trap distribution within the band gap of Si3N4. Our approximation demonstrated better experimental data matching compared to previous work, where the approximation was based on the uniform trap distribution law. Further, we performed another additional sample measurement set of the samples to evaluate the parameters of the low-resistance state (LRS) variations at different temperatures. Analysis of these measurements allowed us to estimate the temperatures at which the samples will retain LRS for 10 years.
Advances in Functional Inorganic Materials Prepared by Wet Chemical Methods (Volume II)
Authors: Zarkov Aleksej , Kareiva Aivaras , Tamasauskaite-Tamasiunaite Loreta
Published: February 2023

Functional inorganic materials are an indispensable part of innovative technologies, which are essential for development in many fields of industry [...]
Threat Analysis of Forest Fragmentation and Degradation for Peruvian Primates
Authors: Shanee Sam , Fernández-Hidalgo Lorena , Allgas Nestor , Vero Veronica , Bello-Santa Cruz Raul , Bowler Mark , Erkenswick Watsa Mrinalini , García Mendoza Gabriel , García-Olaechea Alvaro , Hurtado Cindy , Vega Zoila , Marsh Laura , Boonratana Ramesh , Mendoza A. Patricia
Published: February 2023

Peru has 55 primate taxa (including all species and subspecies), a third of which are threatened. The major drivers of habitat loss, degradation and fragmentation are grazing, forestry, agriculture and transport infrastructure. Other activities such as hunting exacerbate these threats. We assessed the threats from degradation and fragmentation facing Peruvian primates to aid in the design and implementation of mitigation strategies. Through GIS-based mapping, statistical modeling and specialist assessments, we evaluated all primate taxa using the IUCN Conservation Measures Partnership Unified Classifications of Direct Threats across five categories (direct threats to primates, threats to habitat, causes of fragmentation, factors exacerbating fragmentation and threats to primates and habitats as a consequence of fragmentation), highlighting which were most common and most severe. Our results showed that all primate taxa were affected by degradation and fragmentation in Peru. The most common and severe direct threat was hunting, whereas housing and urban development, smallholder crop farming, smallholder grazing and large-scale logging were the most common and severe threats across the other categories. The families Cebidae and Atelidae face the highest overall threat. Our analysis showed that the current IUCN listing of Leontocebus leucogenys [LC] underestimates the true threat level this species faces and that Lagothrix lagothricha tschudii [DD] should be listed under one of the threat categories. In Peru, the need for mitigating the threat of habitat fragmentation is clear. To ensure the survival of Peru’s diverse primate taxa, forest connectivity needs to be maintained or recovered through the protection and restoration of key areas considering their biological and social needs.