Detection of high-risk human papilloma virus in women within the framework of the second stage of the pilot project "ОНКОПОИСКСАХА.РФ" in Yakutia
Authors:   PhD, Associate Professor, M.K. Ammosov North-Eastern Federal University, Afanasieva L.N. , Filippov E.V. ,   PhD in Biology, Head of Molecular Biology Laboratory of the State Budgetary Institution of the Republic of Sakha (Yakutia) Yakut Republican Oncological Dispen-sary, Filippova G.V. ,   PhD in Biology, Pathological and Anatomical laboratory of the State Budgetary Institution of the Yakut Republic of Sakha (Yakutia) Yakut Republican Oncological Dispensary, Senior Re-searcher of the Institute for Biological Problems of Cryolithozone Siberian Branch of Russian Academy of Sciences, Nikiforov P.V. ,   Deputy Chief Physician of the State Budgetary Institution of the Republic of Sakha (Yakutia) Yakut Republican Oncology Dispensary, Associate Professor of the Federal State Autonomous Educational Institution of Higher Education M.K. Ammosov North-Eastern Federal University, Nikolaev T.I. ,   PhD in Medicine, Chief Physician of the State Budgetary Institution of the Republic of Sakha (Yakutia) Yakut Republican Oncology Dispensary, Associate Professor of the Federal State Autonomous Educational Institution of Higher Education M.K. Ammosov North-Eastern Federal University, Larionova N.A. ,   Head of the Cytological Laboratory of the State Budgetary Institution of the State Budg-etary Institution of the Republic of Sakha (Yakutia) Yakut Republican Oncological Dispensary, Struchkova M.S. ,   oncogynecologist of the Department of Oncogynecology Budgetary Institution of the Republic of Sakha (Yakutia) Yakut Republican Oncology Dispensary
Published: January 2025

The study was conducted in 2022 as part of the implementation of the second stage of the pilot project "ОНКОПОИСКСАХА.РФ" (ONCOSEARCH) at the Yakut Republican On-cology Dispensary, which is part of the national project "Healthcare". The project involved 798 women from 10 districts of the Republic of Sakha (Yakutia). The comprehensive examination included cytological testing, detection and genotyping of high carcinogenic risk human papillo-mavirus (HСR-HPV) using polymerase chain reaction, and assessment of viral load. The territori-al distribution of HСR-HPV types was demonstrated. The overall infection rate among women ranged from 6.9% (Tomponsky district) to 25.0% (Oymyakon district), with an average of 14.8%. The majority of infections were of the episomal - non-integrated form of HPV. Differ-ences in infection rates among women of different age groups were observed. The highest pro-portion of HCR-HPV carriers, including integrated forms, was found in the age groups of 20-30 and 31-40 years. High-grade squamous intraepithelial lesions (HSIL) were diagnosed in 2.5% of the examined women, while low-grade squamous intraepithelial lesions (LSIL) were found in 1.8%. One woman was diagnosed with squamous cell carcinoma. The study showed a correlation between viral load, integrated forms of HPV, and cervical intraepithelial neoplasia (CIN). The integrated form of genotype 16 made the largest contribution (50%) to CIN lesions among HCR-HPV-infected individuals.
Keywords: human papillomavirus, screening, age distribution, integrated forms, cervical cancer, cervical intraepithelial neoplasia.