bluuebunny/crossref_metadata_2025 huggingface dataset:
Immunotoxin Screening System: A Rapid and Direct Approach to Obtain Functional Antibodies with Internalization Capacities
Authors: Hamamichi Shusei , Fukuhara Takeshi , Hattori Nobutaka
Published: October 2020

Toxins, while harmful and potentially lethal, have been engineered to develop potent therapeutics including cytotoxins and immunotoxins (ITs), which are modalities with highly selective targeting capabilities. Currently, three cytotoxins and IT are FDA-approved for treatment of multiple forms of hematological cancer, and additional ITs are tested in the clinical trials or at the preclinical level. For next generation of ITs, as well as antibody-mediated drug delivery systems, specific targeting by monoclonal antibodies is critical to enhance efficacies and reduce side effects, and this methodological field remains open to discover potent therapeutic monoclonal antibodies. Here, we describe our application of engineered toxin termed a cell-based IT screening system. This unique screening strategy offers the following advantages: (1) identification of monoclonal antibodies that recognize cell-surface molecules, (2) selection of the antibodies that are internalized into the cells, (3) selection of the antibodies that induce cytotoxicity since they are linked with toxins, and (4) determination of state-specific activities of the antibodies by differential screening under multiple experimental conditions. Since the functional monoclonal antibodies with internalization capacities have been identified successfully, we have pursued their subsequent modifications beyond antibody drug conjugates, resulting in development of immunoliposomes. Collectively, this screening system by using engineered toxin is a versatile platform, which enables straight-forward and rapid selection for discovery of novel functional antibodies.
Dental Implant Failure Rate and Marginal Bone Loss in Transplanted Patients: A Systematic Review and Meta-Analysis
Authors: Paraizo Marcela , Botelho João , Machado Vanessa , Mendes José João , Alves Ricardo , Mascarenhas Paulo , Cardoso José Maria
Published: October 2020

This systematic review investigates the failure rate and marginal bone loss (MBL) of dental implants placed in patients undergoing solid-organ transplant (SOT) compared to healthy controls. Three databases (PubMed, Web of Sciences, and the Cochrane Library) were searched up to June 2020 (PROSPERO CRD42019124896). Case-control and cohort studies reporting data failure rate and marginal bone loss (MBL) of dental implants placed in SOT patients were included. The risk of bias of observational studies was assessed through the Newcastle-Ottawa Scale (NOS). Four case-control studies fulfilled the inclusion criteria; all had low risk of bias. Meta-analyses revealed consistently lower implant failure rate than control populations at patient and implant levels. SOT patients had a significant difference of −18% (p-value < 0.001) MBL compared to healthy patients. SOT status poses no serious threat to implant survival. Overall, this group of patients presented lower levels of dental implant failure rate and marginal bone loss compared to otherwise healthy patients. Further intervention trials with larger sample size and longer follow-ups are necessary to confirm these summarized results.
Null and Timelike Geodesics near the Throats of Phantom Scalar Field Wormholes
Authors: Potashov Ivan , Tchemarina Julia , Tsirulev Alexander
Published: October 2020

We study geodesic motion near the throats of asymptotically flat, static, spherically symmetric traversable wormholes supported by a self-gravitating minimally coupled phantom scalar field with an arbitrary self-interaction potential. We assume that any such wormhole possesses the reflection symmetry with respect to the throat, and consider only its observable “right half”. It turns out that the main features of bound orbits and photon trajectories close to the throats of such wormholes are very different from those near the horizons of black holes. We distinguish between wormholes of two types, the first and second ones, depending on whether the redshift metric function has a minimum or maximum at the throat. First, it turns out that orbits located near the centre of a wormhole of any type exhibit retrograde precession, that is, the angle of pericentre precession is negative. Second, in the case of high accretion activity, wormholes of the first type have the innermost stable circular orbit at the throat while those of the second type have the resting-state stable circular orbit in which test particles are at rest at all times. In our study, we have in mind the possibility that the strongly gravitating objects in the centres of galaxies are wormholes, which can be regarded as an alternative to black holes, and the scalar field can be regarded as a realistic model of dark matter surrounding galactic centres. In this connection, we discuss qualitatively some observational aspects of results obtained in this article.
Gravitational Interaction of Cosmic String with Spinless Particle
Authors: Spirin Pavel
Published: October 2020

We consider the gravitational interaction of spinless relativistic particle and infinitely thin cosmic string within the classical linearized-theory framework. We compute the particle’s motion in the transverse (to the unperturbed string) plane. The reciprocal action of the particle on the cosmic string is also investigated. We derive the retarded solution which includes the longitudinal (with respect to the unperturbed-particle motion) and totally-transverse string perturbations.
Welcome to Uro: A New Open Access Platform for Clinical and Experimental Research and Developments in All Fields of Urology and Andrology
Authors: Cai Tommaso
Published: October 2020

Dear esteemed researchers and colleagues, [...]
Viral Related Tools against SARS-CoV-2
Authors: Fernandez-Garcia Laura , Pacios Olga , González-Bardanca Mónica , Blasco Lucia , Bleriot Inés , Ambroa Antón , López María , Bou German , Tomás Maria
Published: October 2020

At the end of 2019, a new disease appeared and spread all over the world, the COVID-19, produced by the coronavirus SARS-CoV-2. As a consequence of this worldwide health crisis, the scientific community began to redirect their knowledge and resources to fight against it. Here we summarize the recent research on viruses employed as therapy and diagnostic of COVID-19: (i) viral-vector vaccines both in clinical trials and pre-clinical phases; (ii) the use of bacteriophages to find antibodies specific to this virus and some studies of how to use the bacteriophages themselves as a treatment against viral diseases; and finally, (iii) the use of CRISPR-Cas technology both to obtain a fast precise diagnose of the patient and also the possible use of this technology as a cure.
Diversity of tRNA Clusters in the Chloroviruses
Authors: Duncan Garry A. , Dunigan David D. , Van Etten James L.
Published: October 2020

Viruses rely on their host’s translation machinery for the synthesis of their own proteins. Problems belie viral translation when the host has a codon usage bias (CUB) that is different from an infecting virus due to differences in the GC content between the host and virus genomes. Here, we examine the hypothesis that chloroviruses adapted to host CUB by acquisition and selection of tRNAs that at least partially favor their own CUB. The genomes of 41 chloroviruses comprising three clades, each infecting a different algal host, have been sequenced, assembled and annotated. All 41 viruses not only encode tRNAs, but their tRNA genes are located in clusters. While differences were observed between clades and even within clades, seven tRNA genes were common to all three clades of chloroviruses, including the tRNAArg gene, which was found in all 41 chloroviruses. By comparing the codon usage of one chlorovirus algal host, in which the genome has been sequenced and annotated (67% GC content), to that of two of its viruses (40% GC content), we found that the viruses were able to at least partially overcome the host’s CUB by encoding tRNAs that recognize AU-rich codons. Evidence presented herein supports the hypothesis that a chlorovirus tRNA cluster was present in the most recent common ancestor (MRCA) prior to divergence into three clades. In addition, the MRCA encoded a putative isoleucine lysidine synthase (TilS) that remains in 39/41 chloroviruses examined herein, suggesting a strong evolutionary pressure to retain the gene. TilS alters the anticodon of tRNAMet that normally recognizes AUG to then recognize AUA, a codon for isoleucine. This is advantageous to the chloroviruses because the AUA codon is 12–13 times more common in the chloroviruses than their host, further helping the chloroviruses to overcome CUB. Among large DNA viruses infecting eukaryotes, the presence of tRNA genes and tRNA clusters appear to be most common in the Phycodnaviridae and, to a lesser extent, in the Mimiviridae.
Expression Pattern of the SARS-CoV-2 Entry Genes ACE2 and TMPRSS2 in the Respiratory Tract
Authors: Liu Yichuan , Qu Hui-Qi , Qu Jingchun , Tian Lifeng , Hakonarson Hakon
Published: October 2020

To address the expression pattern of the SARS-CoV-2 receptor ACE2 and the viral priming protease TMPRSS2 in the respiratory tract, this study investigated RNA sequencing transcriptome profiling of samples of airway and oral mucosa. As shown, ACE2 has medium levels of expression in both small airway epithelium and masticatory mucosa, and high levels of expression in nasal epithelium. The expression of ACE2 is low in mucosal-associated invariant T (MAIT) cells and cannot be detected in alveolar macrophages. TMPRSS2 is highly expressed in small airway epithelium and nasal epithelium and has lower expression in masticatory mucosa. Our results provide the molecular basis that the nasal mucosa is the most susceptible locus in the respiratory tract for SARS-CoV-2 infection and consequently for subsequent droplet transmission and should be the focus for protection against SARS-CoV-2 infection.
Can Measurements of Inflammatory Biomarkers Be Used to Spot Respiratory Viral Infections?
Authors: Sinha Anirban , Lutter René , Dekker Tamara , Dierdorp Barbara , J. Sterk Peter , Frey Urs , Delgado-Eckert Edgar
Published: October 2020

Accurate detection of human respiratory viral infections is highly topical. We investigated how strongly inflammatory biomarkers (FeNO, eosinophils, neutrophils, and cytokines in nasal lavage fluid) and lung function parameters change upon rhinovirus 16 infection, in order to explore their potential use for infection detection. To this end, within a longitudinal cohort study, healthy and mildly asthmatic volunteers were experimentally inoculated with rhinovirus 16, and time series of these parameters/biomarkers were systematically recorded and compared between the pre- and post-infection phases of the study, which lasted two months and one month, respectively. We found that the parameters’/biomarkers’ ability to discriminate between the infected and the uninfected state varied over the observation time period. Consistently over time, the concentration of cytokines, in nasal lavage fluid, showed moderate to very good discrimination performance, thereby qualifying for disease progression monitoring, whereas lung function and FeNO, while quickly and non-invasively measurable using cheap portable devices (e.g., at airports), performed poorly.
Selective Blockade of TNFR1 Improves Clinical Disease and Bronchoconstriction in Experimental RSV Infection
Authors: Morris Dorothea R. , Ansar Maria , Ivanciuc Teodora , Qu Yue , Casola Antonella , Garofalo Roberto P.
Published: October 2020

Respiratory syncytial virus (RSV) is the leading cause of bronchiolitis in infants and young children. Although some clinical studies have speculated that tumor necrosis factor (TNF)-α is a major contributor of RSV-mediated airway disease, experimental evidence remains unclear or conflicting. TNF-α initiates inflammation and cell death through two distinct receptors: TNF-receptor (TNFR)1 and TNFR2. Here we delineate the function of TNF-α by short-lasting blockade of either receptor in an experimental BALB/c mouse model of RSV infection. We demonstrate that antibody-mediated blockade of TNFR1, but not TNFR2, results in significantly improved clinical disease and bronchoconstriction as well as significant reductions of several inflammatory cytokines and chemokines, including IL-1α, IL-1β, IL-6, Ccl3, Ccl4, and Ccl5. Additionally, TNFR1 blockade was found to significantly reduce neutrophil number and activation status, consistent with the concomitant reduction of pro-neutrophilic chemokines Cxcl1 and Cxcl2. Similar protective activity was also observed when a single-dose of TNFR1 blockade was administered to mice following RSV inoculation, although this treatment resulted in improved alveolar macrophage survival rather than reduced neutrophil activation. Importantly, short-lasting blockade of TNFR1 did not affect RSV peak replication in the lung. This study suggests a potential therapeutic approach for RSV bronchiolitis based on selective blockade of TNFR1.
The Strange Case of BCG and COVID-19: The Verdict Is Still up in the Air
Authors: Gopalaswamy Radha , Ganesan Natarajan , Velmurugan Kalamani , Aravindhan Vivekanandhan , Subbian Selvakumar
Published: October 2020

COVID-19, caused by a novel coronavirus, SARS-CoV-2, contributes significantly to the morbidity and mortality in humans worldwide. In the absence of specific vaccines or therapeutics available, COVID-19 cases are managed empirically with the passive immunity approach and repurposing of drugs used for other conditions. Recently, a concept that bacilli Calmette–Guerin (BCG) vaccination could confer protection against COVID-19 has emerged. The foundation for this widespread attention came from several recent articles, including the one by Miller et al. submitted to MedRxiv, a pre-print server. The authors of this article suggest that a correlation exists between countries with a prolonged national BCG vaccination program and the morbidity/mortality due to COVID-19. Further, clinical BCG vaccination trials are currently ongoing in the Netherlands, Australia, the UK, and Germany with the hope of reducing mortality due to COVID-19. Although BCG vaccination helps protect children against tuberculosis, experimental studies have shown that BCG can also elicit a non-specific immune response against viral and non-mycobacterial infections. Here, we summarize the pros and cons of BCG vaccination and critically analyze the evidence provided for the protective effect of BCG against COVID-19 and highlight the confounding factors in these studies.
A Herpesvirus of Turkey-Based Vector Vaccine Reduces Transmission of Newcastle Disease Virus in Commercial Broiler Chickens with Maternally Derived Antibodies
Authors: Tatár-Kis Timea , Fischer Egil A.J. , Cazaban Christophe , Walkó-Kovács Edit , Homonnay Zalan G. , Velkers Francisca C. , Palya Vilmos , Stegeman J. Arjan
Published: October 2020

Newcastle Disease is one of the most important infectious poultry diseases worldwide and is associated with high morbidity, mortality, and economic loss. In several countries, vaccination is applied to prevent and control outbreaks; however, information on the ability of vaccines to reduce transmission of ND virus (NDV) is sparse. Here we quantified the transmission of velogenic NDV among 42-day-old broilers. Chickens were either vaccinated with a single dose of a vector vaccine expressing the F protein (rHVT-ND) at day-old in the presence of maternally derived antibodies or kept unvaccinated. Seeders were challenged 8 h before the co-mingling with the corresponding contacts from the same group. Infection was monitored by daily testing of cloacal and oro-nasal swabs with reverse transcription-real-time PCR and by serology. Vaccinated birds were completely protected against clinical disease and virus excretion was significantly reduced compared to the unvaccinated controls that all died during the experiment. The reproduction ratio, which is the average number of secondary infections caused by an infectious bird, was significantly lower in the vaccinated group (0.82 (95% CI 0.38–1.75)) than in the unvaccinated group (3.2 (95% CI 2.06–4.96)). Results of this study demonstrate the potential of rHVT-ND vaccine in prevention and control of ND outbreaks.
Selecting Target Antigens for Cancer Vaccine Development
Authors: Buonaguro Luigi , Tagliamonte Maria
Published: October 2020

One of the principal goals of cancer immunotherapy is the development of efficient therapeutic cancer vaccines that are able to elicit an effector as well as memory T cell response specific to tumor antigens. In recent years, the attention has been focused on the personalization of cancer vaccines. However, the efficacy of therapeutic cancer vaccines is still disappointing despite the large number of vaccine strategies targeting different tumors that have been evaluated in recent years. While the preclinical data have frequently shown encouraging results, clinical trials have not provided satisfactory data to date. The main reason for such failures is the complexity of identifying specific target tumor antigens that should be unique or overexpressed only by the tumor cells compared to normal cells. Most of the tumor antigens included in cancer vaccines are non-mutated overexpressed self-antigens, eliciting mainly T cells with low-affinity T cell receptors (TCR) unable to mediate an effective anti-tumor response. In this review, the target tumor antigens employed in recent years in the development of therapeutic cancer vaccine strategies are described, along with potential new classes of tumor antigens such as the human endogenous retroviral elements (HERVs), unconventional antigens, and/or heteroclitic peptides.
Big Data Analytics and Its Role to Support Groundwater Management in the Southern African Development Community
Authors: Gaffoor Zaheed , Pietersen Kevin , Jovanovic Nebo , Bagula Antoine , Kanyerere Thokozani
Published: October 2020

Big data analytics (BDA) is a novel concept focusing on leveraging large volumes of heterogeneous data through advanced analytics to drive information discovery. This paper aims to highlight the potential role BDA can play to improve groundwater management in the Southern African Development Community (SADC) region in Africa. Through a review of the literature, this paper defines the concepts of big data, big data sources in groundwater, big data analytics, big data platforms and framework and how they can be used to support groundwater management in the SADC region. BDA may support groundwater management in SADC region by filling in data gaps and transforming these data into useful information. In recent times, machine learning and artificial intelligence have stood out as a novel tool for data-driven modeling. Managing big data from collection to information delivery requires critical application of selected tools, techniques and methods. Hence, in this paper we present a conceptual framework that can be used to manage the implementation of BDA in a groundwater management context. Then, we highlight challenges limiting the application of BDA which included technological constraints and institutional barriers. In conclusion, the paper shows that sufficient big data exist in groundwater domain and that BDA exists to be used in groundwater sciences thereby providing the basis to further explore data-driven sciences in groundwater management.
Quantitative Analysis of the Sub-Cloud Evaporation of Atmospheric Precipitation and Its Controlling Factors Calculated By D-Excess in an Inland River Basin of China
Authors: Ma Xinggang , Jia Wenxiong , Zhu Guofeng , Wang Shijin
Published: October 2020

Atmospheric precipitation is an important part of the water circle in an inland basin. Based on the analytical results of 149 precipitation samples and corresponding surface meteorological data collected at four sampling sites (Lenglong, Ningchang, Huajian and Xiying) at different elevations in the Xiying river basin on the north slope of Qilian Mountains from May to September 2017, the sub-cloud evaporation in precipitation and its controlling factors are analyzed by the Stewart model. The results show that sub-cloud evaporation led to d-excess value in precipitation decrease and d-excess variation from cloud-base to near surface (Δd) increase with decreasing altitude. The remaining evaporation fraction of raindrop (f) decreases with decreasing altitude. The difference of underlying surface led to a difference change of f and Δd in the Xiying sampling site. For every 1% increase in raindrop evaporation, d-excess value in precipitation decreased by about 0.99‰. In an environment of high relative humidity and low temperature, the slope of the linear relationship between f and Δd is less than 0.99. In contrast, in the environment of low relative humidity and high temperature, the slope is higher than 0.99. In this study, set constant raindrop diameter may affect the calculation accuracy. The Stewart model could have different parameter requirements in different study areas. This research is helpful to understand water cycle and land–atmosphere interactions in Qilian Mountains.
Improved Coastal Erosion Prevention Using a Hybrid Method with an Artificial Coral Reef: Large-Scale 3D Hydraulic Experiment
Authors: Kim Taeyoon , Baek Seungil , Kwon Yongju , Lee Jooyong , Cha Sung Min , Kwon Soonchul
Published: October 2020

Coastal erosion, a worldwide social issue, has garnered substantial attention. Numerous methods have been implemented to control coastal erosion problems; however, the presence of rigid structures limits erosion mitigation, thereby causing various challenges. For instance, in the case of submerged breakwaters, local scour in front of the structure and scour caused by the flow occurring in open inlets affect the subsidence and stability of the structure and can also cause structural failure. To solve these problems, this paper proposes a hybrid method of using a submerged breakwater with an artificial coral reef installation; further, this study evaluates the attenuation of waves and mitigation of sediment transportation through large-scale 3D hydraulic experiments. We found that the hybrid method with an artificial coral reef installed in the open inlet shows excellent wave control and plays a clearly beneficial role in the advancement of the shoreline. The artificial coral reef method reduced the return flow generated by the drag force at the breakwater shoulder and open inlet. In addition, scour at the breakwater shoulder was inhibited by collecting the sand escaping offshore. Simultaneously, scour at the open inlet was also mitigated. The application of the hybrid method compensated for the problems caused by local scour and erosion in the submerged breakwater, thereby leading to the improvement of its function. Therefore, the hybrid method proposed in this paper was determined to be applicable not only for submerged breakwaters, but also for various structures for controlling coastal erosion.
Towards Characterising Microplastic Abundance, Typology and Retention in Mangrove-Dominated Estuaries
Authors: Govender Joelene , Naidoo Trishan , Rajkaran Anusha , Cebekhulu Senzo , Bhugeloo Astika , Sershen Sershen
Published: October 2020

Plastic and, particularly, microplastic (MP) pollution is a growing research theme, dedicated largely to marine systems. Occurring at the land–sea interface, estuarine habitats such as mangroves are at risk of plastic pollution. This study compared MP pollution (level, morphotype, polymer composition, size and colour) across four South African estuaries, in relation to the built and natural environment. Mouth status, surrounding human population densities and land-use practices influenced the level and type of MP pollution. Systems that were most at risk were predominantly open estuaries surrounded by high population densities and diverse land use types. Microplastic levels and the diversity of types detected increased with increasing levels of anthropogenic disturbance. Overall, microfibres dominated in estuarine water (69%) and mangrove sediment (51%). Polyethylene (43%) and polypropylene (23%) were the dominant polymers overall. Weathered fishing gear, weathered packaging items and run-off from urban/industrial centres are probable sources of MP pollution. Increased run-off and river input during the wet/rainy season may explain the markedly higher MP loads in estuarine waters relative to the dry season. By contrast, MP deposition in mangrove sediment was higher during the dry season. Sediment MP abundance was significantly positively correlated with both pneumatophore density and sediment size (500–2000 µm). This study highlights the role of mangroves as MP sinks, which may limit movement of MPs into adjacent environments. However, under conditions such as flooding and extreme wave action, mangroves may shift from sinks to sources of plastic pollution.
An Improved Configuration of Vertical-Flow Mesh Tube Filters for Seawater Pretreatment: Performance, Cleaning, and Energy Consumption
Authors: Kim Dong-Ho , Choi Changkyoo , Lee Chulmin , Adha Rusnang Syamsul , Nguyen Thanh-Tin , Ahn Sang-Jun , Son Hee-Jong , Kim In S.
Published: October 2020

Roughing filters are types of porous media filter used in pretreatment systems where the raw water contains a large amount of suspended particles (SPs) and organic matter. Mesh tube filtration (MTF) media are roughing-filter media composed of low-density polyethylene used for SP removal during wastewater treatment. In this study, we present an improved MTF design—a porous filter bed (PFB), which exhibits superior SP removal performance compared to conventional MTF media. We then compare the applicability of MTF and PFB to both the primary pretreatment process for seawater desalination and the water reuse process. In bench-scale SP removal experiments, PFB shows removal rates of 46.7%, 68.0%, 67.6%, and 68.4% at hydraulic retention times of 15, 20, 30, and 60 min, respectively, which are better than those of MTF. The specific energy consumption (SEC) of batch dissolved air flotation (DAF) was known to range from 0.035 to 0.047 kWh/m3, whereas the SEC calculated for pilot-scale MTF and PFB is 0.027 kWh/m3 and minimum energy for influent supply, respectively. This suggests that PFB can compete with DAF as a primary pretreatment process. MTF predominantly removes SPs by sedimentation, whereas SP removal in PFB typically occurs via deposition of SPs on the mesh tube media.
Response of Agricultural Drought to Meteorological Drought: A Case Study of the Winter Wheat above the Bengbu Sluice in the Huaihe River Basin, China
Authors: Gao Chao , Chen Cai , He Yi , Ruan Tian , Luo Gang , Sun Yanwei
Published: October 2020

This study investigated the responses of winter wheat to drought for the above part of the Bengbu Sluice in the Huaihe River based on the daily scale dataset of 60 meteorological stations from 1961–2015. Crop water deficit index (CWDI) and relative moisture index (M) were used to examine the winter wheat drought and meteorological drought, respectively. We then analyzed the spatial-temporal evolution characteristics of these two kinds of drought to calculate the time lag of winter wheat drought to meteorological drought, and finally discuss the relationship between the time lag of winter wheat drought to meteorological drought and the underlying surface geographical factors, and drew the following conclusions. (1) In terms of time scale, for CWDI, except for the filling and mature period, the CWDI at other growth periods showed a slight downward trend; for M, there was no significant change in the interannual trend of each growth period. In terms of spatial scale, the proportion of above moderate drought level in each station of CWDI and M presented a decreasing feature from north to south. (2) The time lag of winter wheat drought to meteorological drought was the shortest (3.21 days) in the greening and heading period and the longest in the over-wintering period (84.35 days). (3) The correlation between the geographical factors and the time lag of winter wheat drought in each growth period was better than 0.5. The high-value points of the relation between the underlying surface geographical factors and the time lag of winter wheat drought were mostly distributed in the mountainous areas with poor soil field capacity and at a greater depth of shallow groundwater, high elevation and steep slope in the areas with aspects to the east and northeast, and the northern areas with less precipitation and lower temperature.
Water Level Fluctuations and Air Temperatures Affect Common Reed Habitus and Productivity in an Intermittent Wetland Ecosystem
Authors: Gaberščik Alenka , Grašič Mateja , Abram Dragan , Zelnik Igor
Published: October 2020

Lake Cerknica is an intermittent wetland ecosystem with extreme water level fluctuations. It hosts extensive reed stands that have colonized different habitat types. Two different stands were compared: a lake stand not directly influenced by the intermittent River Stržen and a riparian stand near River Stržen. Reed productivity (growth and assimilate allocation) was monitored for these reed stand types over 13 years (2007–2019), and this measurement was compared to monthly water levels and air temperatures. Reeds from the lake reed stand were significantly shorter with a lower shoot density, overall biomass production, and ratio of flowering plants. A correlation analysis revealed stronger and more numerous significant correlations between environmental and reed productivity parameters for the lake reed stand compared to the riparian reed stand. The variabilities of the growth and assimilate allocation parameters in the lake reed stand were both mostly explained by the combined water levels for June and July, which explained 47% and 52% of the variability, respectively. The most influential temperatures were in May, which explained 29% and 19% of the variability of growth and assimilate allocation parameters, respectively. For the riparian reed stand, water levels and temperatures out of the vegetation season appeared more important. Therefore, habitats with permanent water are more suitable for reeds than those with fluctuating water. However, fluctuating water conditions are expected to become more common due to climate change.
Geomorphology of Canyon Outlets in Zrmanja River Estuary and Its Effect on the Holocene Flooding of Semi-enclosed Basins (the Novigrad and Karin Seas, Eastern Adriatic)
Authors: Hasan Ozren , Miko Slobodan , Brunović Dea , Papatheodorou George , Christodolou Dimitris , Ilijanić Nikolina , Geraga Maria
Published: October 2020

Detailed multi-beam bathymetry, sub-bottom acoustic, and side-scan sonar observations of submerged canyons with tufa barriers were used to characterize the Zrmanja River karst estuary on the eastern Adriatic coast, Croatia. This unique karst environment consists of two submerged karst basins (Novigrad Sea and Karin Sea) that are connected with river canyons named Novsko Ždrilo and Karinsko Ždrilo. The combined use of high-resolution geophysical data with legacy topography and bathymetry data in a GIS environment allowed for the description and interpretation of this geomorphological setting in relation to the Holocene sea-level rise. The tufa barriers had a predominant influence on the Holocene flooding dynamics of the canyons and karst basins. Here, we describe the possible river pathways from the basins during the lowstand and the formation of a lengthening estuary during the Holocene sea-level rise. Based on the analyzed morphologies and the relative sea-level curve for the Adriatic Sea, the flooding of the Novsko Ždrilo occurred 9200 years before present (BP) and Karinsko Ždrilo was flooded after 8400 years BP. The combination of high-resolution geophysical methods gave an accurate representation of the karst estuarine seafloor and the flooding of semi-isolated basins due to sea-level rise.
Passive Detection of Phosphorus in Agricultural Tile Waters Using Reactive Hybrid Anion Exchange Resins
Authors: Li Zhe , Chu Maria Librada , Gentry Lowell , Li Ying , Mitchell Corey , Arai Yuji
Published: October 2020

Tile drainage waters carry considerable loads of phosphorus (P) from agricultural fields to rivers and streams in the Midwestern U.S. An innovative and economical approach to monitor dissolved reactive P (DRP) flux in tile waters is needed to understand the extent of P loss in field-scale. In this study, a passive sampling technique was developed using iron oxide-coated polyacrylic/polystyrene anion exchange resins (hybrid resins) a P sink. Laboratory batch adsorption isotherm and kinetic experiments indicated that the hybrid resins had high P adsorption capacity (7.69–19.84 mg/g) and high kinetic performance. The passive sampling method with field-calibrated hybrid polyacrylic resin and hybrid polystyrene resins (sampling rate: 0.1351 and 0.0763 L/h, respectively) predicted the average DRP concentrations of 0.006–0.020 mg/L, which did not differ significantly (p > 0.05) from the auto-sampling data. A rapid increase in DRP concentration during storm events and subsequent flooding events was also predicted well. In conclusion, a passive detection method using iron oxide coated hybrid resins can be recommended for monitoring seasonally fluctuating DRP flux in agricultural waters as long as the hybrid resins are well-calibrated under specific field conditions (e.g., flow rate and concentration range).
Phytoplankton–Macrophyte Interaction in the Lagoon of Venice (Northern Adriatic Sea, Italy)
Authors: Bernardi Aubry Fabrizio , Acri Francesco , Scarpa Gian Marco , Braga Federica
Published: October 2020

The coexistence of phytoplankton and macrophytes in the Lagoon of Venice (Northern Adriatic Sea, Italy) was investigated using in situ data collected monthly as part of International Long Term Ecosystem Research (LTER), together with satellite imagery for the period 1998–2017. The concentrations of chlorophyll a and hydrochemical parameters were measured in three areas of the lagoon, where the expansion of well-developed stands of submerged vegetation was observed by remote sensing. Our results suggest interaction between phytoplankton and macrophytes (macroalgae and seagrasses) in the last few years of the time series, evidenced by decreasing chlorophyll a concentrations in the vicinity of the macrophyte stands. The integration of LTER and remotely sensed data made it possible to evaluate the interaction of macrophytes and phytoplankton at the ecosystem scale for the first time in the Lagoon of Venice.
Climatic Influences on Agricultural Drought Risks Using Semiparametric Kernel Density Estimation
Authors: Gonzalez Cruz Marangely , Hernandez E. Annette , Uddameri Venkatesh
Published: October 2020

A bivariate kernel density estimation (KDE) method was utilized to develop a stochastic framework to assess how agricultural droughts are related to unfavorable meteorological conditions. KDE allows direct estimation of the bivariate cumulative density function which can be used to extract the marginal distributions with minimal subjectivity. The approach provided excellent fits to bivariate relationships between the standardized soil moisture index (SSMI) computed at three- and six-month accumulations and standardized measures of precipitation (P), potential evapotranspiration (PET), and atmospheric water deficit (AWD = P − PET) at 187 stations in the High Plains region of the US overlying the Ogallala Aquifer. The likelihood of an agricultural drought given a precipitation deficit could be as high as 40–65% within the study area during summer months and between 20–55% during winter months. The relationship between agricultural drought risks and precipitation deficits is strongest in the agriculturally intensive central portions of the study area. The conditional risks of agricultural droughts given unfavorable PET conditions are higher in the eastern humid portions than the western arid portions. Unfavorable PET had a higher impact on the six-month standardized soil moisture index (SSMI6) but was also seen to influence three-month SSMI (SSMI3). Dry states as defined by AWD produced higher risks than either P or PET, suggesting that both of these variables influence agricultural droughts. Agricultural drought risks under favorable conditions of AWD were much lower than when AWD was unfavorable. The agricultural drought risks were higher during the winter when AWD was favorable and point to the role of soil characteristics on agricultural droughts. The information provides a drought atlas for an agriculturally important region in the US and, as such, is of practical use to decision makers. The methodology developed here is also generic and can be extended to other regions with considerable ease as the global datasets required are readily available.
Soil Salinity and Moisture Control the Processes of Soil Nitrification and Denitrification in a Riparian Wetlands in an Extremely Arid Regions in Northwestern China
Authors: Meng Yangyang , He Zhibin , Liu Bing , Chen Longfei , Lin Pengfei , Luo Weicheng
Published: October 2020

Soil nitrification and denitrification are key nitrogen (N) removal processes in riparian wetlands in extremely arid regions, but the driving factors of the two N processes in these wetlands are still unclear. We measured soil nitrification and denitrification rates and related environmental properties in a typical riparian wetland in the middle reaches of the Heihe River, northwestern China. Our results showed that rates of soil nitrification and denitrification exhibited moderate variability, ranging from 52.77 to 221.18 μg kg−1 h−1 and 91.25 to 428.26 μg kg−1 h−1, respectively. Soil salinity was high, with mean electrical conductivity (EC) of 6.8 mS cm−1. Soil salinity and moisture were the key factors influencing nitrification and denitrification in this riparian wetland in an extremely arid region. Soil salinity exerted significant inhibitory impact on soil nitrification when EC was > 4.05 mS cm−1. Soil nitrification increased with an increase in soil moisture when soil water content < 27.03% and decreased with an increase in soil moisture when soil water content > 27.03%. Denitrification had a significantly negative relationship with soil salinity, and significantly positive relationship with soil moisture. The interaction of soil salinity and moisture played a central role in regulating soil denitrification. Based on these results, we propose that water consumption of riparian wetlands, and the planting of halophytes, should be increased to reduce soil salinity and increase soil moisture, which is essential for sustaining soil N removal function in riparian wetlands in extremely arid regions.
Run-Out Simulation of a Landslide Triggered by an Increase in the Groundwater Level Using the Material Point Method
Authors: Troncone Antonello , Pugliese Luigi , Conte Enrico
Published: October 2020

Deformation mechanisms of the slopes are commonly schematized in four different stages: pre-failure, failure, post-failure and eventual reactivation. Traditional numerical methods, such as the finite element method and the finite difference method, are commonly employed to analyse the slope response in the pre-failure and failure stages under the assumption of small deformations. On the other hand, these methods are generally unsuitable for simulating the post-failure behaviour due to the occurrence of large deformations that often characterize this stage. The material point method (MPM) is one of the available numerical techniques capable of overcoming this limitation. In this paper, MPM is employed to analyse the post-failure stage of a landslide that occurred at Cook Lake (WY, USA) in 1997, after a long rainy period. Accuracy of the method is assessed by comparing the final geometry of the displaced material detected just after the event, to that provided by the numerical simulation. A satisfactory agreement is obtained between prediction and observation when an increase in the groundwater level due to rainfall is accounted for in the analysis.
Study of Snap Loads for Idealized Mooring Configurations with a Buoy, Inextensible and Elastic Cable Combinations for the Multi-Float M4 Wave Energy Converter
Authors: Stansby Peter , Carpintero Moreno Efrain
Published: October 2020

There has been considerable modelling and wave basin validation of the multi-mode, multi-float, moored wave energy converter M4. The 6 float (2 power take off) (PTO) configuration is considered here with mooring from a buoy with light inextensible cables. Large mean mooring forces and very large peak or snap forces were measured in large waves while the rotational response about the hinges (for power take off in operational conditions) was predominantly linear. Modelling has been extended with elastic mooring cables connected directly to the base of the bow float and to the buoy. The experimental mean force is input to the linear diffraction/radiation model. The device response is effectively unchanged. The peak mooring force and tensions remain large with direct connection to the base of the bow float but are only slightly greater than the mean forces with elastic cables to the buoy, and an elastic hawser provides a further slight reduction. For the largest waves measured, the force was about 10% of the dry weight of the platform. The idealized efficient modelling may inform more detailed design while efficient methods for determining highly nonlinear mean forces remain to be established.
Evaluation of Permeable Brick Pavement on the Reduction of Stormwater Runoff Using a Coupled Hydrological Model
Authors: Fu Xiaoran , Liu Jiahong , Shao Weiwei , Mei Chao , Wang Dong , Yan Wenchang
Published: October 2020

In several cities, permeable brick pavement (PBP) plays a key role in stormwater management. Although various hydrological models can be used to analyze the mitigation efficiency of PBP on rainfall runoff, the majority do not consider the effect of multi-layered pavement on infiltration in urban areas. Therefore, we developed a coupled model to evaluate the potential effect of PBP in reducing stormwater runoff at a watershed scale. Specifically, we compared the hydrological responses (outflow and overflow) of three different PBP scenarios. The potential effects of PBP on peak flow (PF), total volume (TV), and overflow volume (OV) were investigated for 20 design rainstorms with different return periods and durations. Our results indicate that an increase in PBP ratio reduces both PF (4.2–13.5%) and TV (4.2–10.5%) at the outfall as well as the OV (15.4–30.6%) across networks. The mitigation effect of PBP on OV is linearly correlated to storm return period and duration, but the effects on PF and TV are inversely correlated to storm duration. These results provide insight on the effects of infiltration-based infrastructure on urban flooding.
Open-Surface Water Bodies Dynamics Analysis in the Tarim River Basin (North-Western China), Based on Google Earth Engine Cloud Platform
Authors: Chen Jiahao , Kang Tingting , Yang Shuai , Bu Jingyi , Cao Kexin , Gao Yanchun
Published: October 2020

The Tarim River Basin (TRB), located in an arid region, is facing the challenge of increasing water pressure and uncertain impacts of climate change. Many water body identification methods have achieved good results in different application scenarios, but only a few for arid areas. An arid region water detection rule (ARWDR) was proposed by combining vegetation index and water index. Taking computing advantages of the Google Earth Engine (GEE) cloud platform, 56,284 Landsat 5/7/8 optical images in the TRB were used to detect open-surface water bodies and generated a 30-m annual water frequency map from 1992 to 2019. The interannual changes and trends of the water body area were analyzed and the impacts of climatic and anthropogenic drivers on open-surface water body area dynamics were examined. The results show that: (1) ARWDR is suitable for long-term and large-scale water body identification, especially suitable for arid areas lacking vegetation. (2) The permanent water area was 2093.63 km2 and the seasonal water area was 44,242.80 km2, accounting for 4.52% and 95.48% of the total open-surface water area of he TRB, respectively. (3) From 1992 to 2019, the permanent and seasonal water bodies of the TRB all showed an increasing trend, with obvious spatial heterogeneity. (4) Among the effects of human activities and climate change, precipitation has the largest impact on the water area, which can explain 65.3% of the change of water body area. Our findings provide valuable information for the entire TRB’s open-surface water resources planning and management.
Ecosystem Services under Climate Change Impact Water Infrastructure in a Highly Forested Basin
Authors: Li Xupu , Zhang Liwei , J. O’Connor Patrick , Yan Junping , Wang Bin , Liu De Li , Wang Pengtao , Wang Zhuangzhuang , Wan Luwen , Li Yingjie
Published: October 2020

Climate change can have critical impacts on ecosystem services (ESs) and their inter-relationships, especially for water-related services. However, there has been little work done on characterizing the current and future changes in these services and their inter-relationships under a changing climate. Based on the revised universal soil loss equation (RUSLE), the soil conservation service curve number model (SCS-CN), and the improved stochastic weather-generator-based statistical downscaled global climate models (GCMs), we examined two important water-related services, namely, the soil conservation (SC) service and the flood mitigation (FM) service, and their inter-relationship under baseline and future climate scenarios (Representative Concentration Pathways (RCPs) 4.5 and 8.5). We took the Upper Hanjiang River Basin (UHRB), which is the core water source area of the China’s South-to-North Water Diversion Project (S–NWDP), as an illustration. The findings revealed that (1) the SC and FM services will both decrease under the two climate scenarios examined; (2) the SC and FM services showed a significant synergistic inter-relationship and the synergy will be improved by 16.48% and 2.95% under RCP 4.5 and RCP 8.5, respectively, which provides an opportunity for management optimization; (3) the ecological degradation in the UHRB will likely have serious consequences for the middle and lower reaches of the Hanjiang river basin, and therefore impact the actual economic benefits of the S–NWDP. This study points to the necessity for understanding the dynamic changes and inter-relationships of ecosystem services under future climate change and provides information regarding the consequences of climate change, which is useful for policy and infrastructure investment.
Differential Responses of Food Web Properties to Opposite Assembly Rules and Species Richness
Authors: Guo Yulun , Wang Tao , Jorge García Molinos , Zhang Huan , Zhang Peiyu , Zhang Min , Xu Jun
Published: October 2020

Trophic niches condition the energetic performance of species within food webs providing a vital link between food web assembly, species diversity, and functioning of ecosystems. Our understanding of this important link is, however, limited by the lack of empirical tools that can be easily applied to compare entire food webs at regional scales. By comparison, with different a priori synthetic models defined according to specific assembly rules (i.e., purely random, limiting similarity, and niche filtering), we demonstrate that a set of food web properties (trophic richness, evenness, and divergence) are controlled by ecological processes. We further demonstrate that although both limiting similarity and niche filtering are statistically significant assembly processes shaping our studied lake food webs, their relative importance is richness-dependent, and contextual to the specific food web property under consideration. Our results have both important theoretical and practical implications. Theoretically, the observed richness-dependent variation on food web properties contradicts the common criticism on food web theory that food web properties are roughly scale-invariant. Practically, these properties can help avoiding spurious conclusions, while providing useful information for multiple food web niche spaces supporting the ecosystem functioning.