bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantum mechanical modeling of interstellar molecules on cosmic dusts: H2O, NH3, and CO2
Authors: Li Fangfang , Quan Donghui , Zhang Xia , Li Xiaohu , Esimbek Jarken
Published: November 2022

Since the first detection of CH molecule in interstellar medium (ISM), more than 270 molecules have been identified in various astronomical sources in ISM. These molecules include big complex ones, such as fullerene (C60) and polycyclic aromatic hydrocarbons (PAHs), which are the main components of carbonaceous dust. Dust surface chemistry plays an important role in explaining the formation of interstellar molecules. However, many of the dust surface chemical parameters, such as the adsorption energies, are still of uncertainty. Here we present a study of the adsorption of water (H2O), ammonia (NH3), and carbon dioxide (CO2) on graphene-like substrate within the framework of density functional theory (DFT). We used Gaussian 16 software and adopted the corrected generalized gradient approximation (GGA) with the Perdew–Burke–Ernzerhof (PBE) functions. We determined the optimal accretion position of the studied molecules on the graphene-like surface and calculated the adsorption energies. Furthermore, according to the density of states and molecular orbitals of the adsorbed states, we analyzed the charge transfer between the molecules and the graphene-like surface. These results can provide more accurate parameters for calculating the chemical reaction rates on the dust surface, thus contributing to the understanding of dust-surface reactions in ISM.
Reverse effects of Streptococcus mutans physiological states on neutrophil extracellular traps formation as a strategy to escape neutrophil killing
Authors: Huang Lijia , Lu Wenhua , Ning Yang , Liu Jia
Published: November 2022

Bacteria in nature are present in different lifestyles with distinct characteristics. Streptococcus mutans is the etiologic pathogen of dental caries and could easily gain access into the bloodstream after oral surgery and adopt a biofilm lifestyle, resulting in infective endocarditis. A growing amount of evidence have revealed that the large web-like structure composed of extracellular DNA and antimicrobial proteins released by neutrophils, named Neutrophil Extracellular Traps (NETs), play an active role in the defense against bacterial invasion. The present study demonstrated that NETs formation was discriminatively affected by S. mutans biofilm and its planktonic counterpart. The free-floating planktonic S. mutans exhibited an active NETs response, whereas the biofilm community exhibited a reverse negative NETs response. Besides, impaired biofilm killing correlated with the decrease in NETs production. Unlike planktonic cells, biofilm avoided the burst of reactive oxygen species (ROS) when co-culture with neutrophils, and the NADPH-oxidase pathway was partially involved. A mice infection model also supported the distinguishing response of neutrophils challenged by different lifestyles of S. mutans. In conclusion, different bacterial physiological states can affect the distinct response of the host–microbe interaction, thus contributing to the anti-pathogen immune response activation and immune surveillance survival.
Challenges and best practices in corporate AI governance: Lessons from the biopharmaceutical industry
Authors: Mökander Jakob , Sheth Margi , Gersbro-Sundler Mimmi , Blomgren Peder , Floridi Luciano
Published: November 2022

While the use of artificial intelligence (AI) systems promises to bring significant economic and social benefits, it is also coupled with ethical, legal, and technical challenges. Business leaders thus face the question of how to best reap the benefits of automation whilst managing the associated risks. As a first step, many companies have committed themselves to various sets of ethics principles aimed at guiding the design and use of AI systems. So far so good. But how can well-intentioned ethical principles be translated into effective practice? And what challenges await companies that attempt to operationalize AI governance? In this article, we address these questions by drawing on our first-hand experience of shaping and driving the roll-out of AI governance within AstraZeneca, a biopharmaceutical company. The examples we discuss highlight challenges that any organization attempting to operationalize AI governance will have to face. These include questions concerning how to define the material scope of AI governance, how to harmonize standards across decentralized organizations, and how to measure the impact of specific AI governance initiatives. By showcasing how AstraZeneca managed these operational questions, we hope to provide project managers, CIOs, AI practitioners, and data privacy officers responsible for designing and implementing AI governance frameworks within other organizations with generalizable best practices. In essence, companies seeking to operationalize AI governance are encouraged to build on existing policies and governance structures, use pragmatic and action-oriented terminology, focus on risk management in development and procurement, and empower employees through continuous education and change management.
Unusual combination of acute aortic dissection, Mayer-Rokitansky-Küster-Hauser syndrome, and 46,XX gonadal dysgenesis: A case report
Authors: Zeng Yifan , Hu Yerong , Jiang Bo , Tan Ling , Tang Hao
Published: November 2022

Background

Acute Stanford type A aortic dissection (ATAAD) is a life-threatening disease. Elderly patients are the high-risk population for aortic dissection (AD). Young patients with AD usually have heritable connective tissue diseases such as Marfan syndrome and Loeys-Dietz syndrome. However, young AD patients without heritable connective tissue disease are relatively rare.

Case presentation

Herein, we report a case of a 25-year-old female diagnosed with ATAAD accompanied by undeveloped secondary sexual characteristics. Computed tomography angiography (CTA) showed that her AD involved the ascending and abdominal aorta. She had undergone thoracic endovascular aortic stent graft implantation in a local hospital due to acute Stanford type B aortic dissection at age 19. No uterus or ovaries were found on CTA and transabdominal ultrasonography. Sex hormone detection revealed a low estrogen level. G-banded karyotyping analyses revealed a normal 46,XX karyotype. Finally, her abnormalities in the reproductive system were diagnosed as MRKH syndrome and 46,XX gonadal dysgenesis. Whole-exome sequencing (WES) in the patient found an SNP variant of ACTA2 c.773G>A and MYH11 c.5081A>G. MYH11 c.5081A>G was also found in her mother and younger brother. Copy number variations sequencing (CNV-seq) found an approximately 109.30 Kb duplication at chromosome 6p22.3 (Chr 6: g.24920238–25029535) with a copy number of 3. We performed emergent total aortic arch replacement with frozen elephant trunk surgery, and the patient recovered well after surgery. However, her abdominal AD was stilling progression during 6 months of follow-up.

Conclusion

To our knowledge, we report the world's first case of early-onset recurrent AD combined with MRKH syndrome and 46,XX gonadal dysgenesis.
Predictive nomogram for coronary heart disease in patients with type 2 diabetes mellitus
Authors: Xiao Shucai , Dong Youzheng , Huang Bin , Jiang Xinghua
Published: November 2022

Objective

This study aimed to identify risk factors for coronary heart disease (CHD) in patients with type 2 diabetes mellitus (T2DM), build a clinical prediction model, and draw a nomogram.

Study design and methods

Coronary angiography was performed for 1,808 diabetic patients who were recruited at the department of cardiology in The Second Affiliated Hospital of Nanchang University from June 2020 to June 2022. After applying exclusion criteria, 560 patients were finally enrolled in this study and randomly divided into training cohorts (n = 392) and validation cohorts (n = 168). The least absolute shrinkage and selection operator (LASSO) is used to filter features in the training dataset. Finally, we use logical regression to establish a prediction model for the selected features and draw a nomogram.

Results

The discrimination, calibration, and clinical usefulness of the prediction model were evaluated using the c-index, receiver operating characteristic (ROC) curve, calibration chart, and decision curve. The effects of gender, diabetes duration, non-high-density lipoprotein cholesterol, apolipoprotein A1, lipoprotein (a), homocysteine, atherogenic index of plasma (AIP), nerve conduction velocity, and carotid plaque merit further study. The C-index was 0.803 (0.759–0.847) in the training cohort and 0.775 (0.705–0.845) in the validation cohort. In the ROC curve, the Area Under Curve (AUC) of the training set is 0.802, and the AUC of the validation set is 0.753. The calibration curve showed no overfitting of the model. The decision curve analysis (DCA) demonstrated that the nomogram is effective in clinical practice.

Conclusion

Based on clinical information, we established a prediction model for CHD in patients with T2DM.
Both J- and L-shaped upper hemisternotomy approaches are suitable for total arch replacement with frozen elephant trunk in patients with Type A dissection
Authors: Yang Zhonglu , Jiang Hui , Liu Yu , Ge Yuguang , Wang Huishan
Published: November 2022

Background

Minimally invasive total arch replacement (TAR) with frozen elephant trunk (FET) implantation can be carried out through J-, L-, and inverted T-shaped upper ministernotomy. L- and inverted T-shaped upper ministernotomy are selected mostly for their better surgical view compared to J-shaped. However, few studies have paid attention to the difference in clinical effects between J- and L-shaped upper hemisternotomy in acute Type A aortic dissection (ATAAD).

Materials and methods

We retrospectively analyzed 74 consecutive patients with ATAAD who underwent TAR with FET implantation between December 2019 and October 2020. Patients were divided into the L group (n = 31, L-shaped upper hemisternotomy) and the J group (n = 43, J-shaped upper hemisternotomy). Perioperative characteristics were recorded.

Results

No significant difference was found in any of the pre-operative, post-operative, or follow-up variables between the two groups. However, the CPB establishment time in the J group was significantly shorter than that in the L group (65.0 ± 17.9 min vs. 77.9 ± 17.2 min, P < 0.05). Other intraoperative variables showed no significant difference.

Conclusion

Total arch replacement with frozen elephant trunk implantation is feasible and can be carried out safely through J-shaped or L-shaped incision. A J-shaped incision might be beneficial for single incision, while an L-shaped incision might be beneficial if an extra incision is required to achieve better artery perfusion.
Metallogenic mechanism of the Houge’zhuang gold deposit, Jiaodong, China: Evidence from fluid inclusion, in situ trace element, and sulfur isotope compositions
Authors: Chi Naijie , Han Zuozhen , Liu Chuan’e , Zhang Wei , Zhang Yanhui , Shan Wei , Li Zengsheng , Li Min , Wang Xiufeng , Sun Yuqin
Published: November 2022

The Houge’zhuang gold deposit, located in the Penglai–Qixia gold belt of the Jiaodong peninsula, is a representative auriferous quartz vein-style deposit. Pyrite is the most common and main gold-bearing mineral and shows complex textures in the Houge’zhuang gold deposit. Study of ore-related pyrite is of great significance for understanding the metallogenesis of this deposit, especially the gold precipitation mechanism. The present study applied systematic microscopic observation, fluid inclusion analysis, electron microprobes,in situ LA-ICP-MS trace-element analysis, and in situ sulfur isotope analyses. Three types of fluid inclusions were identified, among which the ore-forming fluids exhibited medium–low salinity and temperature, with the fluid inclusions mainly comprising H2O and CO2. Three types of pyrites were identified: 1) Py0, characterized by low concentrations of As and Au and low δ34S values (5.51–6.86‰). 2) Py1, found in the gold-quartz-pyrite veins and homogeneous in chemical composition with no obvious zonal growth but with notably more gold and chalcopyrite inclusions. Py1 contained medium and uniform concentrations of As and was Au-rich, with δ34S values ranging from 7.13 to 7.89‰ (mean 7.44‰). 3) Py2 contained arsenic-bearing pyrite and was found in quartz-polymetallic sulfide veins, with distinct As enrichment and As-rich rims of pyrite and growth zoning. Consequently, the primary ore-forming fluids passed through some arsenic and δ34S-rich sedimentary rocks, such as the Jingshan, Fenzishan, and Penglai groups. With the occurrence of stable water-rock interaction, the extracted fluids were enriched for As and δ34S. Furthermore, Au was closely associated with As, visible gold grains tended to occur in association with Py1 at stage II, and invisible gold was related to the Au-As-rich Py2 of stage III. The As-bearing pyrites shared a close spatiotemporal relationship with gold, playing an important role in the formation and exploration of high-grade gold deposits.
Impacts of moisture supply from the subtropical western Pacific on the subtropical high and the atmospheric river during the heavy rain of 2020 in Japan
Authors: Zhao Ning , Manda Atsuyoshi , Guo Xiaojun , Wang Bin
Published: November 2022

Our recent study suggested that moisture from the subtropical Western Pacific (WP) contributed the most to an atmospheric river (AR) event and the related heavy rainfall during the heavy rain of 2020 in Japan based on a Lagrangian approach. However, the actual role of moisture from the subtropical WP region in the AR and heavy rain formations remains unclear. To evaluate that, we conducted a set of numerical sensitivity experiments by adjusting the surface moisture supply over the subtropical WP region with factors of 0%, 50%, and 200%. The sensitivity experiments suggest that the reduced surface evaporation over the subtropical WP suppressed the local convective activity and decreased moisture content in the whole troposphere, leading to shallow and weak positive geopotential height anomalies. Although the slightly strengthened WP subtropical High (WPSH) and related anomalous anticyclonic circulation enhanced the southwesterly wind, convective activities along the Meiyu-Baiu front were still weakened due to the largely reduced moisture supply, resulting in another anomalous anticyclonic circulation over Japan but had much stronger and deeper structures. These two anomalous circulations and the reduced moisture modulated the AR over Japan, which eventually caused the weakened rainfalls and the northward migration of the rainband. By contrast, larger surface evaporation enhanced the local convective activities and weakened the dominant WPSH, resulting in the weakening and the southward migration of the AR. Overall, this study confirmed the large contributions of moisture supply from the subtropical WP region to the AR and related precipitation over Japan during the record-breaking Meiyu-Baiu season in 2020 via both dynamic and thermodynamic influences. In addition, it reveals that, although larger evaporation over the WP region would increase the total rainfalls but would not have led to more several rainfalls over certain regions, such as the relatively small Kyushu Island.
Exploring the land use and land cover change in the period 2005–2020 in the province of Errachidia, the pre-sahara of Morocco
Authors: Karmaoui Ahmed , Ben Salem Abdelkrim , El Jaafari Samir , Chaachouay Hassan , Moumane Adil , Hajji Lhoussain
Published: November 2022

The study investigates the land use and land cover (LULC) in Errachidia province (Pre-Saharan of Morocco) in the period 2005–2020. To this end, remote sensing (RS) tools such as LULC, the normalized difference vegetation index (NDVI), the normalized difference water index (NDWI), the Enhanced Vegetation Index (EVI), Gravity Recovery and Climate Experiment (GRACE) satellite data, and precipitations were processed and combined. The monitoring of LULC over this period reveals different changes in this area. Generally, for water bodies, two trends can be observed, an increasing trend since 2006 and a decreasing trend from 2011 to the present. However, an increasing trend was recorded for the urban-and-built-up-lands and the grasslands categories. NDVI, NDWI, and EVI showed three major peaks recorded in the same periods (in 2007, 2009, and 2015). In terms of water storage, three phases were found. The first depicted the lowest groundwater quantities with a decreasing trend, which corresponds to a period of drought and/or human pressure. The second phase, the most important that recorded the greatest storage of water while the third phase corresponds to low storage with a decreasing trend. LULC of croplands reveals a slightly increasing trend from 2012 to 2020, which shows an orientation to an extension of crops mainly of date palm encouraged by government programs. A strong correlation between the seasonal NDVI and water storage using GRACE-Data for the period was found. In the end, recommendations on the adaptation to CC were proposed. The findings demonstrate that RS techniques are useful tools to detect hydrological droughts, observe, and conserve land resources. In arid areas such as Errachidia, the solution lies in rationalizing the use of water resources protecting them from uncontrolled anthropogenic events, which may alleviate the pressure. To support local sustainable development, environmental scientists and decision-makers may use the outputs of this study.
Strength and seismic anisotropy of textured FeSi at planetary core conditions
Authors: Kolesnikov E. , Kupenko I. , Achorner M. , Plückthun C. , Liermann H.-P. , Merkel S. , Sanchez-Valle C.
Published: November 2022

Elastic anisotropy of iron-bearing alloys and compounds can lead to a variation of seismic velocities along different directions in planetary cores. Understanding the deformation properties of candidate core-forming materials is thus necessary to reveal the details about the interior of distant planets. Silicon has been considered to be one of the dominant light elements in the cores. Here we investigated the deformation of the ε-FeSi phase up to 49 GPa and 1100 K employing the radial X-ray diffraction technique in diamond anvil cells. Stoichiometric FeSi is a good approximation for the deformation behavior of the Fe-FeSi system and the low-pressure polymorph of FeSi may be the stable phase in the cores of small terrestrial planets such as Mercury. Yield strength in ε-FeSi is higher than in hcp-Fe and hcp-Fe-Si alloys, in the temperature range we investigated here the temperature has little influence on the lattice strain parameters, yield strength, and anisotropy within experimental precision. The azimuthal anisotropy of the longitudinal sound waves in ε-FeSi is below 0.6% at low pressure and decreases further with compression, while the shear wave contrast is below 1.25% in the entire investigated pressure range. Therefore, polycrystalline aggregates of iron silicide are nearly isotropic at extreme conditions. Consequently, any observed anisotropy in planetary cores will be incompatible with silicon being the dominant light element in the core composition.
Reframing neurodiversity in engineering education
Authors: Chrysochoou Maria , Zaghi Arash E. , Syharat Connie Mosher
Published: November 2022

A growing body of literature suggests that neurodiverse learners may possess assets that are highly desirable within engineering disciplines. Even so, despite the potential of neurodiverse individuals to contribute to innovation in science and engineering, neurodiverse students, such as those with attention deficit hyperactivity disorder (ADHD), autism, or dyslexia, remain highly underrepresented in engineering majors. We argue that the predominant perception of neurodiversity as a disability limits the participation of neurodiverse students in engineering education, ultimately impacting the diversity and creativity of the engineering workforce. In this paper, we review the emerging literature on neurodiversity that takes a social ecology approach and moves away from deficit-based models. We then describe the potential benefits and challenges of neurodiversity in the context of engineering education. We conclude with a concept analysis of how a strengths-based perspective of neurodiversity may be integrated within engineering education in particular, as well as in higher education overall, as we present our vision for a transformative education system that moves beyond mere accommodation of learning differences and empowers all students to leverage their unique strengths. In presenting a strengths-based approach to neurodiversity, we aim to contribute to a paradigm shift that transforms how university faculty and staff understand and perceive neurodiversity, improves the educational experiences of neurodiverse students in higher education and enhances the creativity of the engineering workforce.
Can we abandon foregut exclusion for an ideal and safe metabolic surgery?
Authors: Widjaja Jason , Chu Yuxiao , Yang Jianjun , Wang Jian , Gu Yan
Published: November 2022

Foregut (foregut exclusions) and hindgut (rapid transit of nutrients to the distal intestine) theories are the most commonly used explanations for the metabolic improvements observed after metabolic surgeries. However, several procedures that do not comprise duodenal exclusions, such as sleeve with jejunojejunal bypass, ileal interposition, and transit bipartition and sleeve gastrectomy were found to have similar diabetes remission rates when compared with duodenal exclusion procedures, such as gastric bypass, biliopancreatic diversion with duodenal switch, and diverted sleeve with ileal interposition. Moreover, the complete exclusion of the proximal intestine could result in the malabsorption of several important micronutrients. This article reviews commonly performed procedures, with and without foregut exclusion, to better comprehend whether there is a critical need to include foregut exclusion in metabolic surgery.
Oocyte phenotype, genetic diagnosis, and clinical outcome in case of patients with oocyte maturation arrest
Authors: Zhu Lixia , Yang Qiyu , Jin Huizi , Zhou Juepu , Wang Meng , Yang Liu , Li Zhou , Qian Kun , Jin Lei
Published: November 2022

Background

oocyte maturation arrest (OMA) is currently one of the major causes of in vitro fertilization (IVF) failure, and several gene mutations were found to be associated with OMA. The purpose of this study was to identify the oocyte phenotype, genetic diagnosis, and clinical outcomes of patients with OMA and explore their possible interrelationships, thus providing a more individualized and efficient treatment strategy guidance accordingly.

Methods

A retrospective study was conducted, involving 28 infertile women with OMA in the Reproductive Medicine Center of Tongji Hospital from 2018 to 2021. Whole-exome sequencing was performed for the detection of gene mutations. Patients were classified into three groups based on their oocyte phenotype, and for each group, the immature oocytes were cultured in vitro and mature oocytes were fertilized to evaluate both the maturation capacity and developmental potential. The clinical outcomes of OMA patients with different gene mutations or from different groups were further analyzed and compared.

Results

Twenty-eight women with OMA were evaluated in this study. According to the stage of OMA, 14 (50.0%) women were classified as OMA Type-1 (GV arrest), 5 (17.9%) were OMA Type-2 (MI arrest), and 9 (32.1%) were OMA Type-3 (with both GV and MI arrest). Immature oocytes from OMA patients exhibited significantly lower maturation rates even after IVM, compared to those in general patients. Seven patients (25.0%) were detected to have deleterious variations in two genes (PATL2 and TUBB8), known to be associated with the OMA phenotype. Patients with identified mutations were found to have little opportunity to obtain offspring with their own oocytes. Among the patients without mutations identified, those classified as OMA Type-1 or Type-3 still had a chance to obtain offspring through IVF or natural pregnancy, while all patients in the Type-2 group failed to obtain live birth.

Conclusions

Three different phenotypes were observed in patients with OMA. The clinical outcomes of patients were associated with the presence of gene mutations and the classification of oocyte phenotype, thus a reasonable triage system was proposed to optimize the allocation of health care resources and maximize patient benefit.
Smartphone-microfluidic fluorescence imaging system for studying islet physiology
Authors: Yu Xiaoyu , Xing Yuan , Zhang Yiyu , Zhang Pu , He Yi , Ghamsari Farid , Ramasubramanian Melur K. , Wang Yong , Ai Huiwang , Oberholzer Jose
Published: November 2022

Smartphone technology has been recently applied for biomedical image acquisition and data analysis due to its high-quality imaging capability, and flexibility to customize multi-purpose apps. In this work, we developed and characterized a smartphone-microfluidic fluorescence imaging system for studying the physiology of pancreatic islets. We further evaluated the system capability by performing real-time fluorescence imaging on mouse islets labeled with either chemical fluorescence dyes or genetically encoded fluorescent protein indicators (GEFPIs). Our results showed that the system was capable of analyzing key beta-cell insulin stimulator-release coupling factors in response to various stimuli with high-resolution dynamics. Furthermore, the integration of a microfluidics allowed high-resolution detection of insulin secretion at single islet level. When compared to conventional fluorescence microscopes and macro islet perifusion apparatus, the system has the advantages of low cost, portable, and easy to operate. With all of these features, we envision that this smartphone-microfluidic fluorescence imaging system can be applied to study islet physiology and clinical applications.
Syndromic and non-syndromic etiologies causing neonatal hypocalcemic seizures
Authors: Huang Yi-Chieh , Chao Yin-Chi , Lee Inn-Chi
Published: November 2022

Background

The diagnosis of neonatal hypocalcemic seizures (HS) in newborns is made based on clinical signs and serum calcium level. Their etiology is broad and diverse, and timely detection and initiation of treatment is essential.

Methods

We retrospectively reviewed 1029 patients admitted to the neonatal intensive care unit. Neonatal HS were diagnosed in 16 patients, and we compared etiologies and clinical outcomes, including clinical seizures and neurodevelopment at least over 1 year old.

Results

The etiologies can be broadly categorized into 5 syndromic and 11 non-syndromic neonatal HS. Syndromic neonatal HS included 3 Digeorge syndrome, 1 Kleefstra syndrome and 1 Alström syndrome. Non-syndromic neonatal HS included 8 vitamin D deficiency, 1 hypoparathyroidism, and 2 hypoxic-ischemic encephalopathy. Patients with syndromic neonatal HS were found to have worse clinical outcomes than those with nonsyndromic HS. In eight patients with vitamin D deficiency, neurodevelopment was normal. Five of five patients (100%) with syndromic HS used two or more antiseizure drugs. However, among patients with non-syndromic neonatal HS, only one of 11 (9.1%) used more than one drug (p = 0.001).

Conclusion

This finding highlighted that syndromic hypocalcemic seizures in newborns have worse neurodevelopmental outcomes and are more often difficult to manage, and would benefit from a genetic diagnostic approach.
Dynamic spillovers and asymmetric connectedness between fossil energy and green financial markets: Evidence from China
Authors: Deng Jing , Guan Siying , Zheng Huike , Xing Xiaoyun , Liu Cheng
Published: November 2022

With the purpose of risk management for fossil energy investors, this paper examines the dynamic spillover effect and asymmetric connectedness between fossil energy, green financial and major traditional financial markets in China. By employing the spillover index model of Diebold and Yilmaz, a weak correlation between green financial and fossil energy markets is verified, and the market connectedness remains relatively calm despite the COVID-19 pandemic outbreak. Specifically, green bonds receives fewer shocks from crude oil than coal, green stocks receive fewer shocks from coal than crude oil. In addition, rather than the safe-haven characteristics presented by gold, this paper further proves that green bonds also have the potential to act as safe-haven assets, due to the fact that the connectedness between green bonds and energy markets is at low levels. Finally, the magnitude of return spillovers between markets would vary significantly during different periods. The results obtained in this paper have practical implications for both investors and policymakers.
Analysis of the spatial and temporal heterogeneity of factors influencing CO2 emissions in China’s construction industry based on the geographically and temporally weighted regression model: Evidence from 30 provinces in China
Authors: Li Tiantian , Gao Haidong , Yu Jing
Published: November 2022

With the rapid economic development in recent years, China has increased its investment in infrastructure construction, and the construction industry has become a significant contributor to China’s carbon dioxide (CO2) emissions. Therefore, carbon emission reduction in the construction industry is crucial to achieving the goal of “carbon peaking and carbon neutrality” as soon as possible. However, few studies have investigated the factors influencing CO2 emissions from the construction industry in terms of spatial and temporal differences. To address this gap, we first improve the calculation method for the construction industry’s life-cycle assessment (LCA). The geographically and temporally weighted regression (GTWR) model is then utilized to provide insight into the spatio-temporal heterogeneity of the various factors influencing CO2 emissions across other regions and times. The results show that: 1) CO2 emissions from the construction industry in China increased rapidly from 576.5 million tons (Mt) in 2004–3,230 Mt in 2012 and then gradually decreased to 1998.51 Mt in 2020; indirect CO2 emissions accounted for more than 90% of the total CO2 emissions after 2008. 2) There is a solid global positive correlation between CO2 emissions from the construction industry in China during most of the time, and the spatial distribution of CO2 emissions shows a northeast-southwest pattern, with the center of gravity gradually shifting from central China to the southwest. 3) Economic output and industrial agglomeration are positive factors for the increase of CO2 emissions from the construction industry; and urbanization level, production efficiency, and energy efficiency are inhibiting factors for the increase of CO2 emissions from the construction industry. But the contribution and trend of each influencing factor differed significantly across time and regions, showing substantial spatial and temporal heterogeneity. Our findings provide a scientific basis for the Chinese government to implement a regional carbon reduction strategy for the construction industry.
Multiple stressor effects of a heatwave and a herbicide on zooplankton communities: Implications of global climate change
Authors: Roth Sabrina K. , Polazzo Francesco , García-Astillero Ariadna , Cherta Laura , Sobek Anna , Rico Andreu
Published: November 2022

Aquatic ecosystems are exposed to pesticides through various pathways such as spray-drift, agricultural runoff, and chemical spills. Understanding the impact of pesticides on freshwater ecosystems requires not only understanding how pesticides affect aquatic organisms but also knowledge of their interactions with other stressors, such as those related to global climate change. Heatwaves are extended periods of temperature increase relative to the climatological mean. They are increasing in frequency and magnitude and pose an emerging threat to shallow freshwater ecosystems. In this study, we evaluated the single and combined effects of the herbicide terbuthylazine and a simulated heatwave on freshwater zooplankton communities using indoor microcosms. Terbuthylazine was applied at an environmentally relevant concentration (15 µg/L). The heatwave consisted of an increase of 6°C above the control temperature for a period of 7 days. When applied individually, the heatwave increased the total abundance of zooplankton by 3 times. The terbuthylazine exposure led to an indirect effect on the zooplankton community structure, reducing the relative abundance of some taxa. The combination of the heatwave and terbuthylazine had no significant impact on the zooplankton community, indicating additive effects dominated by the herbicide. The interaction between the two stressors increased chlorophyll-a concentrations and apparently changed the structure of the phytoplankton community, which may have benefitted cyanobacteria over green algae. Overall, this study shows that understanding the effects of chemical and non-chemical stressors on aquatic communities remains a challenging task. Further studies should be conducted to improve our mechanistic understanding of multiple stressor interactions at different levels of biological organisation.
Effect of non-essential amino acids (proline and glutamic acid) and sugar polyol (sorbitol) on brood of honey bees
Authors: Noor-ul-Ane Muhammad , Jung Chuleui
Published: November 2022

Dietary nutrients provide fuel for the growth and development of insects as well as chemicals for their tissue construction. Apis mellifera L., an important pollinator, collects nectar and pollens from different plants to get their nutritional needs. Honey bees use protein for growth and development and carbohydrates as energy sources. Pollens predominantly contain proline and glutamic acid (non-essential amino acids). This is the first study to evaluate the role of proline, glutamic acid and sorbitol on bee broods. The composition of the diet can optimize the in vitro rearing process. Therefore, we elaborated on the possible impact of these amino acids and sugar alcohol on bee broods. This study aimed to achieve this objective by rearing honey bee larvae under different concentrations of proline, glutamic acid, and sorbitol (1, 4 and 8%), which were supplemented into the standard larval diet. The supplementation of proline helped the quick development of larvae and pupae of honey bees, whereas developmental time only decreased in pupae in the case of glutamic acid. The duration of the total bee brood development was the shortest (20.1 and 20.6 days) on Pro8 and Glu4, respectively. Proline only increased larvae survival (93.8%), whereas glutamic acid did not increase the survival of any brood stage. Pupal and adult weights were also increased with proline and glutamic acid-supplemented diets. Sorbitol did not change the developmental period of the honey bee brood but increased larval survival (93.7%) only at the lowest concentration (Sor1). The small concentration of sorbitol can be used to increase the survival of the honey bee brood. However, a higher concentration (Sor8) of sorbitol reduced the body weight of both pupae and adults. This study predicted that rearing bee brood could be one of the factors for the selectivity of pollen with higher proline and glutamic acid during the foraging of bees.
Lipid residues in ancient pastoralist pottery from Kazakhstan reveal regional differences in cooking practices
Authors: Murakami Natsuki , Onggaruly Akhan , Rakhimzhanova Saule , Standall Edward A. , Talbot Helen M. , Lucquin Alexandre , Suzuki Miho , Karimagambetov Arhat , Nuskabay Abdinur , Nam Sang-Won , Craig Oliver E. , Shoda Shinya
Published: November 2022

The Bronze Age—Iron Age transition in Central Asia (ca. 800 BCE) was a period of significant cultural change that was heavily influenced by greater population interaction and mobility. Indeed, scholars have increasingly emphasized the role that “food globalization in prehistory” has played in defining this period. In the mountain foothills of Kazakhstan, culinary traditions from across Eurasia were combined through the use of Southwest Asian wheat, barley, and livestock (cattle, sheep, and goats) with East Asian foxtail and broomcorn millets. The development of food cultures during this period has been investigated by archaeobotanical and isotopic analysis, yet lipid residues from pottery, which directly represent culinary practices, have not been adequately examined. In this study, lipid residue analysis was conducted on 72 pottery sherds, excavated from three burial mounds and one non-burial, ritual site located in Kazakhstan, dating to ca. 700–200 BCE. A particularly informative observation was the frequency of miliacin, a biomarker of broomcorn millet, detected in residues that corresponded well with previously published regional differences observed in carbon isotope ratios of human remains that indicate the consumption of C4 plants. This study also demonstrates continuity of Bronze Age dairying traditions into the Iron Age. Finally, this study sheds new light on the diversity of food cultures and mortuary practices in this region, which were not uniform across either space or time.
CRISPR nuclease off-target activity and mitigation strategies
Authors: Wienert Beeke , Cromer M. Kyle
Published: November 2022

The discovery of CRISPR has allowed site-specific genomic modification to become a reality and this technology is now being applied in a number of human clinical trials. While this technology has demonstrated impressive efficacy in the clinic to date, there remains the potential for unintended on- and off-target effects of CRISPR nuclease activity. A variety of in silico-based prediction tools and empirically derived experimental methods have been developed to identify the most common unintended effect—small insertions and deletions at genomic sites with homology to the guide RNA. However, large-scale aberrations have recently been reported such as translocations, inversions, deletions, and even chromothripsis. These are more difficult to detect using current workflows indicating a major unmet need in the field. In this review we summarize potential sequencing-based solutions that may be able to detect these large-scale effects even at low frequencies of occurrence. In addition, many of the current clinical trials using CRISPR involve ex vivo isolation of a patient’s own stem cells, modification, and re-transplantation. However, there is growing interest in direct, in vivo delivery of genome editing tools. While this strategy has the potential to address disease in cell types that are not amenable to ex vivo manipulation, in vivo editing has only one desired outcome—on-target editing in the cell type of interest. CRISPR activity in unintended cell types (both on- and off-target) is therefore a major safety as well as ethical concern in tissues that could enable germline transmission. In this review, we have summarized the strengths and weaknesses of current editing and delivery tools and potential improvements to off-target and off-tissue CRISPR activity detection. We have also outlined potential mitigation strategies that will ensure that the safety of CRISPR keeps pace with efficacy, a necessary requirement if this technology is to realize its full translational potential.
Examining the effects of Salmonella phage on the caecal microbiota and metabolome features in Salmonella-free broilers
Authors: Lorenzo-Rebenaque Laura , Casto-Rebollo Cristina , Diretto Gianfranco , Frusciante Sarah , Rodríguez Juan Carlos , Ventero María-Paz , Molina-Pardines Carmen , Vega Santiago , Marin Clara , Marco-Jiménez Francisco
Published: November 2022

Bacteriophages selectively infect and kill their target bacterial host, being a promising approach to controlling zoonotic bacteria in poultry production. To ensure confidence in its use, fundamental questions of safety and toxicity monitoring of phage therapy should be raised. Due to its high specificity, a minimal impact on the gut ecology is expected; however, more in-depth research into key parameters that influence the success of phage interventions has been needed to reach a consensus on the impact of bacteriophage therapy in the gut. In this context, this study aimed to investigate the interaction of phages with animals; more specifically, we compared the caecum microbiome and metabolome after a Salmonella phage challenge in Salmonella-free broilers, evaluating the role of the phage administration route. To this end, we employed 45 caecum content samples from a previous study where Salmonella phages were administered via drinking water or feed for 24 h from 4, 5 to 6-weeks-old broilers. High-throughput 16S rRNA gene sequencing showed a high level of similarity (beta diversity) but revealed a significant change in alpha diversity between broilers with Salmonella-phage administered in the drinking water and control. Our results showed that the phages affected only a few genera of the microbiota’s structure, regardless of the administration route. Among these, we found a significant increase in Streptococcus and Sellimonas in the drinking water and Lactobacillus, Anaeroplasma and Clostridia_vadinBB60_group in the feed. Nevertheless, the LC-HRMS-based metabolomics analyses revealed that despite few genera were significantly affected, a substantial number of metabolites, especially in the phage administered in the drinking water were significantly altered (64 and 14 in the drinking water and feed groups, respectively). Overall, our study shows that preventive therapy with bacteriophages minimally alters the caecal microbiota but significantly impacts their metabolites, regardless of the route of administration.
Intelligence, education level, and risk of Parkinson’s disease in European populations: A Mendelian randomization study
Authors: Shi Jingjing , Tian Jie , Fan Yu , Hao Xiaoyan , Li Mengjie , Li Jiadi , Ma Dongrui , Guo Mengnan , Li Shuangjie , Xu Yuming , Shi Changhe
Published: November 2022

Background: A high level of education or intelligence (IQ) is reported to be a risk factor for Parkinson’s disease (PD). The purpose of this study was to systematically examine the causal relationships between IQ, educational attainment (EA), cognitive performance, and PD.

Methods: We used summary statistics from genome-wide association studies on IQ, EA, cognitive performance, and PD. Four genome-wide association study (GWAS) data for PD were used to comprehensively explore the causal relationship, including PD GWAS (regardless of sex), age at onset of PD GWAS, male with PD GWAS, and female with PD GWAS data. We conducted a two sample Mendelian randomization (MR) study using the inverse-variance weighted (IVW), weighted median, simple mode, and weighted mode methods to evaluate the causal association between these factors. MR-Egger and MR-PRESSO were used for sensitivity analysis to test and correct horizontal pleiotropy. Multivariate MR (MVMR) was also used to account for the covariation between IQ, EA, and cognition, as well as to explore potential mediating factors.

Results: Genetically predicted higher IQ was associated with an increased risk of PD in the entire population, regardless of gender. In the analyses using the IVW method, the odds ratio was 1.37 (p = 0.0064). Men with a higher IQ, more years of education, or stronger cognitive ability are more likely to develop PD compared to women. MVMR showed that adjusting for education and cognition largely attenuated the association between IQ and PD, suggesting that education and cognition may mediate the effect of IQ on PD.

Conclusion: This study provides genetic support for the causal link between higher IQ and an increased risk of PD.
Autologous organoid co-culture model reveals T cell-driven epithelial cell death in Crohn’s Disease
Authors: Hammoudi Nassim , Hamoudi Sarah , Bonnereau Julie , Bottois Hugo , Pérez Kevin , Bezault Madeleine , Hassid Déborah , Chardiny Victor , Grand Céline , Gergaud Brice , Bonnet Joëlle , Chedouba Leila , Tran Minh My-Linh , Gornet Jean-Marc , Baudry Clotilde , Corte Hélène , Maggiori Léon , Toubert Antoine , McBride Jacqueline , Brochier Camille , Neighbors Margaret , Le Bourhis Lionel , Allez Matthieu
Published: November 2022

Lympho-epithelial interactions between intestinal T resident memory cells (Trm) and the epithelium have been associated with inflammatory bowel disease (IBD) activity. We developed ex vivo autologous organoid-mucosal T cell cocultures to functionally assess lymphoepithelial interactions in Crohn’s Disease (CD) patients compared to controls. We demonstrate the direct epithelial cell death induced by autologous mucosal T cells in CD patients but not in controls. These findings were positively correlated with T cell infiltration of the organoids. This potential was inhibited by limiting lympho-epithelial interactions through CD103 and NKG2D blocking antibodies. These data directly demonstrate for the first time the direct deleterious effect of mucosal T cells on the epithelium of CD patients. Such ex-vivo models are promising techniques to unravel the pathophysiology of these diseases and the potential mode of action of current and future therapies.
Ascaris suum excretory/secretory products differentially modulate porcine dendritic cell subsets
Authors: Hamid Benjamin , Ebner Friederike , Bechtold Lalita , Kundik Arkadi , Rausch Sebastian , Hartmann Susanne
Published: November 2022

Helminths produce excretory/secretory products (E/S) which can modulate the immune responses of their hosts. Dendritic cells (DC) are essential for initiating the host T cell response and are thus potential targets for modulation by helminth E/S. Here we study immunomodulation of porcine peripheral blood DC subsets following ex vivo stimulation with E/S from Ascaris suum, a common helminth of pigs with considerable public health and economic importance. Our data showed that the relative frequencies of DC subsets in porcine blood differ, with plasmacytoid DC (pDC) being the most prominent in healthy 6-month-old pigs. pDC are an important cytokine source, and we found that A. suum E/S suppressed production of the type 1 cytokines IL-12p40 and TNF-α by this subset following toll-like receptor (TLR) ligation. In contrast, conventional DC (cDC) are more efficient antigen presenters, and the expression of CD80/86, costimulatory molecules essential for efficient antigen presentation, were modulated differentially by A. suum E/S between cDC subsets. CD80/86 expression by type 1 cDC (cDC1) following TLR ligation was greatly suppressed by the addition of A. suum E/S, while CD80/86 expression by type 2 cDC (cDC2) was upregulated by A. suum E/S. Further, we found that IFN-γ production by natural killer (NK) cells following IL-12 and IL-18 stimulation was suppressed by A. suum E/S. Finally, in the presence of E/S, IFN-γ production by CD4+ T cells co-cultured with autologous blood-derived DC was significantly impaired. Together, these data provide a coherent picture regarding the regulation of type 1 responses by A. suum E/S. Responsiveness of pDC and cDC1 to microbial ligands is reduced in the presence of E/S, effector functions of Th1 cells are impaired, and cytokine-driven IFN-γ release by NK cells is limited.