bluuebunny/crossref_metadata_2025 huggingface dataset:
Repeatome Analysis and Satellite DNA Chromosome Patterns in Hedysarum Species
Authors: Yurkevich Olga Yu. , Samatadze Tatiana E. , Zoshchuk Svyatoslav A. , Semenov Alexey R. , Morozov Alexander I. , Selyutina Inessa Yu. , Amosova Alexandra V. , Muravenko Olga V.
Published: November 2024

The cosmopolitan genus Hedysarum L. (Fabaceae) is divided into sections Hedysarum, Stracheya, and Multicaulia. This genus includes many valuable medicinal, melliferous, and forage species. The species taxonomy and genome relationships within the sections are still unclear. We examined intra- and interspecific diversity in the section (sect.) Hedysarum based on repeatome analyses using NGS data, bioinformatic technologies, and chromosome FISH mapping of 35S rDNA, 5S rDNA, and the identified satellite DNA families (satDNAs). A comparison of repeatomes of H. alpinum, H. theinum, and H. flavescens revealed differences in their composition. However, similarity in sequences of most satDNAs indicated a close relationship between genomes within sect. Hedysarum. New effective satDNA chromosomal markers were detected, which is important for karyotype analyses within Hedysarum. Intra- and interspecific variability in the chromosomal distribution patterns of the studied markers were revealed, and species karyograms were constructed. These results provided new insight into the karyotype structures and genomic diversity within sect. Hedysarum, clarified the systematic position of H. sachalinense and H. arcticum, and confirmed the distant genomic relationships between species from sections Hedysarum and Multicaulia. Our findings are important for further comparative genome studies within the genus Hedysarum.
Identification of Key Genes Involved in Seed Germination of Astragalus mongholicus
Authors: Li Junlin , Guo Shuhong , Zhang Xian , He Yuhao , Wang Yaoqin , Tian Hongling , Zhang Qiong
Published: November 2024

Seed germination is a fundamental process in plant reproduction, and it involves a series of complex physiological mechanisms. The germination rate of Astragalus mongholicus (AM) seeds is significantly lower under natural conditions. To investigate the key genes associated with AM seed germination, seeds from AM plants were collected at 0, 12, 24, and 48 h for a transcriptomic analysis, weighted gene co-expression network analysis (WGCNA), and machine learning (ML) analysis. The primary pathways involved in AM seed germination include plant-pathogen interactions and plant hormone signaling. Four key genes were identified through the WGCNA and ML: Cluster-28,554.0, FAS4, T10O24.10, and EPSIN2. These findings were validated using real-time quantitative reverse transcription PCR (qRT-PCR), and results from RNA sequencing demonstrated a high degree of concordance. This study reveals, for the first time, the key genes related to AM seed germination, providing potential gene targets for further research. The discovery of N4-acetylcysteine (ac4C) modification during seed germination not only enhances our understanding of plant ac4C but also offers valuable insights for future functional research and application exploration.
Diversity of Neurotransmitter-Producing Human Skin Commensals
Authors: Rahmdel Samane , Purkayastha Moushumi , Nega Mulugeta , Liberini Elisa , Li Ningna , Luqman Arif , Brüggemann Holger , Götz Friedrich
Published: November 2024

Recent findings indicate that human microbiota can excrete trace amines, dopamine, and serotonin. These neurotransmitters (NTs) can either affect classical neurotransmitter signaling or directly trigger trace amine-associated receptors (TAARs), with still unclear consequences for host physiology. Compared to gut microbiota, less information is available on the role of skin microbiota in NT production. To explore this, 1909 skin isolates, mainly from the genera Staphylococcus, Bacillus, and Corynebacterium, were tested for NT production. Only 6.7% of the isolates were capable of producing NTs, all of which belonged to the Staphylococcus genus. Based on substrate specificity, we identified two distinct profiles among the NT producers. One group primarily produced tryptamine (TRY) and phenylethylamine (PEA), while the other mainly produced tyramine (TYM) and dopamine (Dopa). These differing production profiles could be attributed to the activity of two distinct aromatic amino acid decarboxylase enzymes, SadA and TDC, responsible for generating the TRY/PEA and TYM/Dopa product spectra, respectively. SadA and TDC orthologues differ in structure and size; SadA has approximately 475 amino acids, whereas the TDC type consists of about 620 amino acids. The genomic localization of the respective genes also varies: tdc genes are typically found in small, conserved gene clusters, while sadA genes are not. The heterologous expression of sadA and tdc in Escherichia coli yielded the same product spectrum as the parent strains. The possible effects of skin microbiota-derived NTs on neuroreceptor signaling in the human host remain to be investigated.
Proinflammatory Cytokines Enhance the Mineralization, Proliferation, and Metabolic Activity of Primary Human Osteoblast-like Cells
Authors: Bousch Juliana Franziska , Beyersdorf Christoph , Schultz Katharina , Windolf Joachim , Suschek Christoph Viktor , Maus Uwe
Published: November 2024

Osteoporosis is a progressive metabolic bone disease characterized by decreased bone density and microarchitectural deterioration, leading to an increased risk of fracture, particularly in postmenopausal women and the elderly. Increasing evidence suggests that inflammatory processes play a key role in the pathogenesis of osteoporosis and are strongly associated with the activation of osteoclasts, the cells responsible for bone resorption. In the present study, we investigated, for the first time, the influence of proinflammatory cytokines on the osteogenic differentiation, proliferation, and metabolic activity of primary human osteoblast-like cells (OBs) derived from the femoral heads of elderly patients. We found that all the proinflammatory cytokines, IL-1β, TNF-α, IL-6, and IL-8, enhanced the extracellular matrix mineralization of OBs under differentiation-induced cell culture conditions. In the cases of IL-1β and TNF-α, increased mineralization was correlated with increased osteoblast proliferation. Additionally, IL-1β- and TNF-α-increased osteogenesis was accompanied by a rise in energy metabolism due to improved glycolysis or mitochondrial respiration. In conclusion, we show here, for the first time, that, in contrast to findings obtained with cell lines, mesenchymal stem cells, or animal models, human OBs obtained from patients exhibited significantly enhanced osteogenesis upon exposure to proinflammatory cytokines, probably in part via a mechanism involving enhanced cellular energy metabolism. This study significantly contributes to the field of osteoimmunology by examining a clinically relevant cell model that can help to develop treatments for inflammation-related metabolic bone diseases.
Changes in the Composition and Diversity of the Intestinal Microbiota Associated with Carbohydrate Consumption in Type 2 Diabetes Mellitus Patients
Authors: Martínez-Carrillo Beatriz Elina , De Sales-Millán Amapola , Aguirre-Garrido José Félix , Valdés-Ramos Roxana , de María Cruz-Estrada Flor , Castillo-Cardiel José Arturo
Published: November 2024

Type 2 diabetes mellitus (T2DM) is a multifactorial disease, influenced by dietary and environmental factors that can modify the intestinal microbiota. The aim of this study was to evaluate changes in the composition and diversity of the intestinal microbiota associated with carbohydrate (CHO) consumption in T2DM patients. Forty patients participated, with and without T2DM. Fecal samples were collected for the characterization of microbial diversity from the massive sequencing of the 16S rRNA gene. Carbohydrate consumption was quantified using the Frequency Consumption Foods questionnaire (FCF), the groups were categorized according to Body Mass Index (BMI) and BMI + CHO consumption. The group without T2DM showed normal biochemical and anthropometric parameters, although they had a high carbohydrate consumption compared to the group with T2DM. At the phylum level, there were differences in relative abundance; the control overweight group (CL–OW > CHO) and T2DM-Normal Weight > CHO patients had increased Bacteroides and decreased Firmicutes. In contrast, the CL–OW > CHO and T2DM-OW < CHO patients, showed reduced Bacteroidetes and an elevated amount of Firmicutes. At the genus level, the differences were in the relative abundance of Roseburia, Clostridium_IV, Prevotella, and Sporobacter, associated with the consumption of carbohydrates. The groups that consumed high amounts of carbohydrates, regardless of whether they had diabetes mellitus or were overweight, had a significantly reduced proportion of Faecalibacterium, an altered proportion of Bacteroides. The high consumption of carbohydrates showed considerable modifications in the composition and diversity of the bacterial communities.
Computational Investigation of the Therapeutic Potential of Detarium senegalense in the Management of Erectile Dysfunction
Authors: Ejeje Jerius Nkwuda , Agbebi Emmanuel Ayodeji , Mathenjwa-Goqo Makhosazana Siduduzile , Oje Obinna Aru , Agboinghale Precious Eseose , Ebe Ikechukwu Theophilus , Obafemi Tajudeen Olabisi , Adewole Ezekiel , Omaka Omaka N. , Onikanni Sunday Amos , Ajiboye Basiru Olaitan , Omotuyi Olaposi Idowu , Oyinloye Babatunji Emmanuel
Published: November 2024

Erectile dysfunction (ED) is a multifactorial social problem affecting men worldwide. While phosphodiesterase type 5 inhibitors (PDE5) like sildenafil are commonly used, they often present side effects, underscoring the need for alternative therapies. Therefore, this study investigated the potential of phytochemicals from Detarium senegalense in the management of ED. A library of phytochemicals from Detarium senegalense was generated, prepared, and interacted with six key enzymes implicated in ED, including PDE5, using the Schrödinger Maestro suite. The results identified catechin, epicatechin, and gallic acid as the leading compounds with significant binding affinities for the targeted enzymes. Catechin and epicatechin (−9.877 and −11.408 kcal/mol, respectively) exhibited comparable binding affinities to sildenafil (−11.926 kcal/mol) on PDE5. The MD simulation results also revealed superior stability and ability to maintain interaction with key amino acids at the active site of PDE5 over the entire simulation period for these compounds. These compounds also demonstrated favorable ADMET profiles over sildenafil, including high gastrointestinal absorption and no violation of Lipinski’s rule, indicating good bioavailability and drug likeness. These findings suggest that flavonoids from Detarium senegalense, especially catechin and epicatechin, have potential in the management of ED by interacting with multiple targets involved in its pathogenesis.
Special Issue “Adenosine Receptors in Health and Disease”
Authors: Vlajkovic Srdjan M.
Published: November 2024

Adenosine receptor signalling regulates various physiological processes throughout the body [...]
Tat-Beclin-1 Peptide Ameliorates Metabolic Dysfunction-Associated Steatotic Liver Disease by Enhancing Hepatic Autophagy
Authors: Chen Chun-Liang , Huang Fen-Fen , Lin Hsueh-Fang , Wu Chi-Chien , Ni Yen-Hsuan , Lin Yu-Cheng
Published: November 2024

Autophagy plays a crucial role in hepatic lipid metabolism, making it a key therapeutic target for addressing metabolic dysfunction-associated steatotic liver disease (MASLD). This study evaluates the efficacy of the Tat-Beclin-1 (TB-1) peptide, a specific autophagy inducer, in mitigating MASLD. Initially, we examined the impact of the TB-1 peptide on autophagic activity and intracellular lipid metabolism in HepG2 cells treated with oleic acid, using a Tat scrambled (TS) control peptide for comparison. Subsequently, we established a MASLD mouse model by feeding a high-fat diet (HFD) for 16 weeks, followed by intraperitoneal administration of TB-1 or TS. Assessments included liver histopathology, serum biochemistry, and autophagy marker analysis. Our findings indicate that the TB-1 peptide significantly increased the LC3II/β-actin ratio in a dose- and time-dependent manner while promoting the expression of key autophagy markers Beclin-1 and ATG5-12. Furthermore, TB-1 treatment led to a marked reduction in both the size and number of lipid droplets in HepG2 cells. In vivo, HFD-fed mice exhibited increased liver weight, elevated serum alanine aminotransferase levels, and impaired oral glucose tolerance. TB-1 administration effectively mitigated these hepatic and metabolic disturbances. Histological analysis further revealed a substantial reduction in the severity of hepatic steatosis and fibrosis in TB-1-treated mice compared to TS controls. In conclusion, the TB-1 peptide shows significant potential in reducing the severity of MASLD in both HepG2 cell models and HFD-induced MASLD mouse models. Enhancing autophagy through TB-1 represents a promising therapeutic strategy for treating MASLD.
Transcriptomic and Metabolomics Joint Analyses Reveal the Influence of Gene and Metabolite Expression in Blood on the Lactation Performance of Dual-Purpose Cattle (Bos taurus)
Authors: Ma Shengchao , Wang Dan , Zhang Menghua , Xu Lei , Fu Xuefeng , Zhang Tao , Yan Mengjie , Huang Xixia
Published: November 2024

Blood is an important component for maintaining animal lives and synthesizing sugars, lipids, and proteins in organs. Revealing the relationship between genes and metabolite expression and milk somatic cell count (SCC), milk fat percentage, milk protein percentage, and lactose percentage in blood is helpful for understanding the molecular regulation mechanism of milk formation. Therefore, we separated the buffy coat and plasma from the blood of Xinjiang Brown cattle (XJBC) and Chinese Simmental cattle (CSC), which exhibit high and low SCC/milk fat percentage/milk protein percentage/lactose percentages, respectively. The expression of genes in blood and the metabolites in plasma was detected via RNA-Seq and LC-MS/MS, respectively. Based on the weighted gene coexpression network analysis (WGCNA) and functional enrichment analysis of differentially expressed genes (DEGs), we further found that the expression of genes in the blood mainly affected the SCC and milk fat percentage. Immune or inflammatory-response-related pathways were involved in the regulation of SCC, milk fat percentage, milk protein percentage, and lactose percentage. The joint analysis of the metabolome and transcriptome further indicated that, in blood, the metabolism pathways of purine, glutathione, glycerophospholipid, glycine, arginine, and proline are also associated with SCC, while lipid metabolism and amino-acid-related metabolism pathways are associated with milk fat percentage and milk protein percentage, respectively. Finally, related SCC, milk fat percentage, and milk protein percentage DEGs and DEMs were mainly identified in the blood.
Co-Aggregation of TDP-43 with Other Pathogenic Proteins and Their Co-Pathologies in Neurodegenerative Diseases
Authors: Jiang Lei-Lei , Zhang Xiang-Le , Hu Hong-Yu
Published: November 2024

Pathological aggregation of a specific protein into insoluble aggregates is a common hallmark of various neurodegenerative diseases (NDDs). In the earlier literature, each NDD is characterized by the aggregation of one or two pathogenic proteins, which can serve as disease-specific biomarkers. The aggregation of these specific proteins is thought to be a major cause of or deleterious result in most NDDs. However, accumulating evidence shows that a pathogenic protein can interact and co-aggregate with other pathogenic proteins in different NDDs, thereby contributing to disease onset and progression synergistically. During the past years, more than one type of NDD has been found to co-exist in some individuals, which may increase the complexity and pathogenicity of these diseases. This article reviews and discusses the biochemical characteristics and molecular mechanisms underlying the co-aggregation and co-pathologies associated with TDP-43 pathology. The TDP-43 aggregates, as a hallmark of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD), can often be detected in other NDDs, such as Alzheimer’s disease (AD), Parkinson’s disease (PD), Huntington’s disease (HD) and spinocerebellar ataxia type 2 (SCA2). In many cases, TDP-43 is shown to interact and co-aggregate with multiple pathogenic proteins in vitro and in vivo. Furthermore, the co-occurrence and co-aggregation of TDP-43 with other pathogenic proteins have important consequences that may aggravate the diseases. Thus, the current viewpoint that the co-aggregation of TDP-43 with other pathogenic proteins in NDDs and their relevance to disease progression may gain insights into the patho-mechanisms and therapeutic potential of various NDDs.
A Critical Review of Recent Advances in Maize Stress Molecular Biology
Authors: Meng Lingbo , Zhang Jian , Clarke Nicholas
Published: November 2024

With the intensification of global climate change and environmental stress, research on abiotic and biotic stress resistance in maize is particularly important. High temperatures and drought, low temperatures, heavy metals, salinization, and diseases are widespread stress factors that can reduce maize yields and are a focus of maize-breeding research. Molecular biology provides new opportunities for the study of maize and other plants. This article reviews the physiological and biochemical responses of maize to high temperatures and drought, low temperatures, heavy metals, salinization, and diseases, as well as the molecular mechanisms associated with them. Special attention is given to key transcription factors in signal transduction pathways and their roles in regulating maize stress adaptability. In addition, the application of transcriptomics, genome-wide association studies (GWAS), and QTL technology provides new strategies for the identification of molecular markers and genes for maize-stress-resistance traits. Crop genetic improvements through gene editing technologies such as the CRISPR/Cas system provide a new avenue for the development of new stress-resistant varieties. These studies not only help to understand the molecular basis of maize stress responses but also provide important scientific evidence for improving crop tolerance through molecular biological methods.
A Snapshot of Early Transcriptional Changes Accompanying the Pro-Neural Phenotype Switch by NGN2, ASCL1, SOX2, and MSI1 in Human Fibroblasts: An RNA-Seq Study
Authors: Samoilova Ekaterina M. , Chudakova Daria A. , Dashinimaev Erdem B. , Snezhkina Anastasiya V. , Kudryashova Olga M. , Lipatova Anastasia V. , Soboleva Alesya V. , Vorob’yev Pavel O. , Valuev-Elliston Vladimir T. , Zakirova Natalia F. , Ivanov Alexander V. , Baklaushev Vladimir P.
Published: November 2024

Direct pro-neural reprogramming is a conversion of differentiated somatic cells to neural cells without an intermediate pluripotency stage. It is usually achieved via ectopic expression (EE) of certain transcription factors (TFs) or other reprogramming factors (RFs). Determining the transcriptional changes (TCs) caused by particular RFs in a given cell line enables an informed approach to reprogramming initiation. Here, we characterized TCs in the human fibroblast cell line LF1 on the 5th day after EE of the single well-known pro-neural RFs NGN2, ASCL1, SOX2, and MSI1. As assessed by expression analysis of the bona fide neuronal markers nestin and beta-III tubulin, all four RFs initiated pro-neuronal phenotype conversion; analysis by RNA-seq revealed striking differences in the resulting TCs, although some pathways were overlapping. ASCL1 and SOX2 were not sufficient to induce significant pro-neural phenotype switches using our EE system. NGN2 induced TCs indicative of cell phenotype changes towards neural crest cells, neural stem cells, mature neurons, as well as radial glia, astrocytes, and oligodendrocyte precursors and their mature forms. MSI1 mainly induced a switch towards early stem-like cells, such as radial glia.
Response Surface Methodology Approach for the Prediction and Optimization of the Mechanical Properties of Sustainable Laterized Concrete Incorporating Eco-Friendly Calcium Carbide Waste
Authors: Khalid Auwal Ahmad , Gora Abdurra’uf. M. , Rafindadi A. D. , Haruna Sadi I. , Ibrahim Yasser E.
Published: November 2024

This study investigated the combined effects of calcium carbide waste (CCW) and lateritic soil (LS) on sustainable concrete’s fresh and mechanical properties as a construction material for infrastructure development. The study will explore the possibility of using easily accessible materials, such as lateritic soils and calcium carbide waste. Therefore, laterite soil was used to replace some portions of fine aggregate at 0% to 40% (interval of 10%) by weight, while CCW substituted the cement content at 0%, 5%, 10%, 15%, and 20% by weight. A response surface methodology/central composite design (RSM/CCD) tool was applied to design and develop statistical models for predicting and optimizing the properties of the sustainable concrete. The LS and CCW were input variables, and compressive strength and splitting tensile properties are response variables. The results indicated that the combined effects of CCW and LS improve workability by 18.2% compared to the control mixture. Regarding the mechanical properties, the synergic effects of CCW as a cementitious material and LS as a fine aggregate have improved the concrete’s compressive and splitting tensile strengths. The contribution of LS is more pronounced than that of CCW. The established models have successfully predicted the mechanical behavior and fresh properties of sustainable concrete utilizing LS and CCW as the independent variables with high accuracy. The optimized responses can be achieved with 15% CCW and 10% lateritic soil as a substitute for fine aggregate weight. These optimization outcomes produced the most robust possible results, with a desirability of 81.3%.
Methyl-Beta-Cyclodextrin Restores Aberrant Bone Morphogenetic Protein 2-Signaling in Bone Marrow Stromal Cells Obtained from Aged C57BL/6 Mice
Authors: Halloran Daniel , Pandit Venu , Chukwuocha Kelechi , Nohe Anja
Published: November 2024

During aging, disruptions in various signaling pathways become more common. Some older patients will exhibit irregular bone morphogenetic protein (BMP) signaling, which can lead to osteoporosis (OP)—a debilitating bone disease resulting from an imbalance between osteoblasts and osteoclasts. In 2002, the Food and Drug Administration (FDA) approved recombinant human BMP-2 (rhBMP-2) for use in spinal fusion surgeries as it is required for bone formation. However, complications with rhBMP-2 arose and primary osteoblasts from OP patients often fail to respond to BMP-2. Although patient samples are available for study, previous medical histories can impact results. Consequently, the C57BL/6 mouse line serves as a valuable model for studying OP and aging. We find that BMP receptor type Ia (BMPRIa) is upregulated in the bone marrow stromal cells (BMSCs) of 15-month-old mice, consistent with prior data. Furthermore, conjugating BMP-2 with Quantum Dots (QDot®s) allows effective binding to BMPRIa, creating a fluorescent tag for BMP-2. Furthermore, after treating BMSCs with methyl-β-cyclodextrin (MβCD), a disruptor of cellular endocytosis, BMP signaling is restored in 15-month-old mice, as shown by von Kossa assays. MβCD has the potential to restore BMPRIa function, and the BMP signaling pathway offers a promising avenue for future OP therapies.
Can Perceived Exertion and Velocity Loss Serve as Indirect Indicators of Muscle Fatigue During Explosive Back Squat Exercise?
Authors: Zhao Hanye , Kurokawa Takanori , Tajima Masayoshi , Liu Zijian , Okada Junichi
Published: November 2024

Background: Muscle fatigue is inevitable during resistance exercises, making its monitoring essential for maintaining athletic performance and reducing the risk of injury. Ratings of perceived exertion (RPE) and velocity loss have been reported as reliable indicators of muscle fatigue during explosive resistance exercises. However, their validity has been assessed only indirectly. This study aimed to directly examine the validity of RPE and velocity loss as markers of muscle fatigue during explosive back squat (BS) exercises. Methods: Seventeen trained men performed three BS tasks with varying volumes (low, medium, high) at 65% of their one-repetition maximum. RPE, spectral fatigue index (SFI), and velocity loss were measured throughout the tasks. Results: Significant effects were observed across conditions for overall RPE (p < 0.001) and velocity loss (p < 0.001), while no significant effect was found for SFI. RPE and SFI increased significantly as the tasks progressed (p < 0.001), while velocity did not significantly decrease. Significant but weak correlations were found between RPE and SFI (r = 0.325, p < 0.001) and between velocity loss and SFI (r = 0.224, p < 0.001). Conclusions: These findings suggest that RPE and muscle fatigue levels increase correspondingly, indicating that RPE could serve as a practical, indirect fatigue marker for explosive BS exercises. However, velocity loss may not fully reflect muscle fatigue during lower-body explosive training and should not be used as the sole indicator. Additionally, caution is warranted when applying velocity-related parameters as indirect physiological markers for resistance exercises. The significant but weak correlations between RPE, velocity loss, and SFI suggest that assessing muscle fatigue in lower-body exercises remains challenging.
Effects of an Instability Training Program Using Global Versus Selective Instability Devices on Dynamic Balance and Ankle Stability in Young Amateur Soccer Players
Authors: Sánchez-Barbadora Mariana , Moreno-Segura Noemí , Alepuz-Moner Vicente , Martín-San Agustín Rodrigo
Published: November 2024

Background/Objectives: Both Sides Utilized it is one of the most employed global instability devices (GID), but it is difficult to progress and select a particular foot structure. In this sense, the Blackboard has been created as selective instability device (SID). The aim of this study is to compare the effects of both devices on balance and ankle stability. Methods: The study was designed as a randomized controlled clinical trial. Twenty healthy amateur soccer players were divided into two groups: GID and SID. Both performed balance training (4-weeks, 3 days/week). Ankle balance and stability were assessed. Paired t-tests were used to analyze the pre-, post-, and between-groups differences. Results: No differences were found between the groups. Significant intra-group changes were found in both groups for posterolateral balance and summation. Moreover, posteromedial balance increased in the GID group. No changes were found in ankle stability results. Conclusions: A balance intervention using GID or SID is effective in improving general and posterolateral balance. Moreover, the GID intervention improved posteromedial balance.
Effect of Body Position on Electrical Activity of Respiratory Muscles During Mouth and Nasal Maximal Respiratory Pressure in Healthy Adults: A Pilot Study
Authors: da Silva Lailane Saturnino , Vieira Rayane Grayce da Silva , Wanderley e Lima Thiago Bezerra , Resqueti Vanessa Regiane , Vilaro Jordi , Fonseca Jessica Danielle Medeiros da , Ribeiro-Samora Giane Amorim , Fregonezi Guilherme Augusto de Freitas
Published: November 2024

Background: This study aimed to analyze the impact of seated, 45° inclined, and supine positions on respiratory muscle strength (Maximal Inspiratory Pressure—MIP, Maximal Expiratory Pressure—MEP, Sniff Nasal Inspiratory Pressure—SNIP and Sniff Nasal Expiratory Pressure—SNEP) and the electrical activity of respiratory muscles in healthy adults. Ten healthy subjects were evaluated. Methods: Personal, anthropometric data (weight, height, BMI) and lung function (spirometry) were collected, followed by random assessments of inspiratory (MIP, SNIP) and expiratory (MEP, SNEP) muscle strength. Respiratory muscle strength maneuvers and surface electromyographic (sEMG) activity were assessed in sitting, 45° inclined, and supine positions. Results: present that MIP was statistically higher in the sitting position compared to the supine position (p < 0.05) and the 45° supine position (p < 0.05), with SNIP: p < 0.05 and SNEP: p < 0.05 as well. Intercostal muscle activity was higher during MIP, MEP, and SNEP maneuvers in the sitting position (p < 0.05). Additionally, rectus abdominis muscle activity was higher in this position during MIP and SNEP maneuvers. Conclusions: The results suggest there are significant differences in inspiratory pressures between positions, with the difference in activity muscle pattern. In conclusion, body position affected maximal respiratory pressures and influences EMG activation of specific respiratory muscles during MIP.
Is Countermovement Jump an Indirect Marker of Neuromuscular Mechanism? Relationship with Isometric Knee Extension Test
Authors: Aedo-Muñoz Esteban , Pérez-Contreras Jorge , Bustamante-Garrido Alejandro , Arriagada-Tarifeño David , Cancino-Jiménez Jorge , Retamal-Espinoza Manuel , Argothy-Buchelli Rodrigo , Brito Ciro , Merino-Muñoz Pablo
Published: November 2024

Several studies have shown that force application is influenced by different neuromuscular mechanisms depending on the time of force application analysis in isometric knee extension test (IKE), and a countermovement jump (CMJ) has contributions from knee extension, so some CMJ variables could be indicators of such mechanisms. Purpose: The aim of this study was to determine the level of relationship of variables of IKE and bilateral CMJ tests. Methods: Male college soccer players (n = 25; corporal mass = 72 ± 8 kg; height = 171 ± 5 cm; age = 22 ± 2 years) performed the IKE at two angles (60° and 75°) on an isokinetic machine and the CMJ on two uniaxial force platforms. To determine the level of relationship, Pearson’s correlation coefficient was analyzed between the test variables. Results: Trivial to moderate correlations (r = −0.45 to 0.62; p < 0.05) were found between CMJ variables and IKE in both knee angles (60° and 75°); Conclusions: The variables of IKE have a trivial to moderate correlation with the variables of CMJ, so the variables of CMJ could not be considered interchangeably with those of IKE and therefore considered indicators of neuromuscular mechanisms isolated from the knee extensor function. Longitudinal design (fatigue or training protocols) should be realized to corroborate these results.
Editorial on the Special Issue “Fluorescence Imaging and Analysis of Cellular Systems”
Authors: Sharma Ashutosh
Published: November 2024

Fluorescence imaging has indeed become a cornerstone in modern cell biology due to its ability to offer highly sensitive, specific, and real-time visualization of cellular structures and dynamic processes [...]
A Review of Magnetic Abrasive Finishing for the Internal Surfaces of Metal Additive Manufactured Parts
Authors: Wang Liaoyuan , Sun Yuli , Xiao Zhongmin , Yang Fanxuan , Kang Shijie , Liu Yanlei , Zuo Dunwen
Published: November 2024

With the rapid development of high-end manufacturing industries such as aerospace and national defense, the demand for metal additive manufactured parts with complex internal cavities has been steadily increasing. However, the finishing of complex internal surfaces, especially for irregularly shaped parts, remains a significant challenge due to their intricate geometries. Through a comparative analysis of common finishing methods, the distinctive characteristics and applicability of magnetic abrasive finishing (MAF) are highlighted. To meet the finishing needs of complex metal additive manufactured parts, this paper reviews the current research on magnetic abrasive finishing devices, processing mechanisms, the development of magnetic abrasives, and the MAF processes for intricate internal cavities. Future development trends in MAF for complex internal cavities in additive manufactured parts are also explored; these are (1) investigating multi-technology composite magnetic abrasive finishing equipment designed for complex internal surfaces; (2) studying the dynamic behavior of multiple magnetic abrasive particles in complex cavities and their material removal mechanisms; (3) developing high-performance magnetic abrasives suitable for demanding conditions; and (4) exploring the MAF process for intricate internal surfaces.
Underwater Network Time Synchronization Method Based on Probabilistic Graphical Models
Authors: Ouyang Yujie , Han Yunfeng , Wang Zeyu , He Yifei
Published: November 2024

In underwater clustering and benchmark networks, nodes need to reduce the rate and energy consumption of acoustic communication while ensuring synchronization accuracy. In large-scale networks, the improvement in the efficiency of existing network time synchronization often relies on the optimization of topological structures, and the improvement in efficiency within local areas is limited. This paper proposes a method to synchronize underwater time using the probability graph model. The method utilizes the positional and motion status information of sensor networks to construct a factor graph model for distributed network synchronization. By simplifying the marginal probability density function of the system clock difference, it can quickly calculate the clock difference parameters of nodes, thereby effectively improve the synchronization efficiency. The experimental results show that the method can complete global time synchronization within a cycle while achieving a clock difference correction accuracy higher than seconds, which significantly optimized the synchronization cycle and efficiency, and reduced the energy consumption of the acoustic communication.
Effect of High-Stress Levels on the Shear Behavior of Geosynthetic-Reinforced Marine Coral Sands
Authors: Liu Lixing , Chen Zhixiong , Ding Xuanming , Ou Qiang
Published: November 2024

As an important construction material, the mechanical and deformation properties of marine coral sand determine the safety and stability of related island and coastal engineering construction. The porous and easily broken characteristics of coral sand often make it difficult to meet engineering construction needs. In particular, coral sand undergoes a large amount of particle breakage under high-stress conditions, which in turn negatively affects its mechanical and deformation properties. In this study, the macro- and micro-mechanical behavior of geosynthetic-reinforced coral sand under high confining pressure was investigated and compared with unreinforced cases using the three-dimensional discrete element method (DEM), which was verified by indoor triaxial tests. The results showed that the stress–strain responses of unreinforced and reinforced coral sand under high confining pressure showed completely different trends, i.e., the hardening tendency shown in the reinforced case. Geosynthetic reinforcement can significantly inhibit the stress–strain softening and bulging deformation of coral sand under high confining pressure, thus improving the shear mechanical performance of the reinforced sample. At the microscopic scale, high confining pressure and reinforcement affected the contact force distribution pattern and stress level between particles, determining the macroscopic mechanical and deformation performance. In addition, the breakage of particles under high confining pressure was mainly affected by shear strain and reinforcement. The particle fragment distribution, particle gradation, and relative breakage index exhibited different trends at different confining pressure levels. These breakage characteristics were closely related to the deformation and stress levels of unreinforced and reinforced samples.
Modal Parameter Identification of Jacket-Type Offshore Wind Turbines Under Operating Conditions
Authors: Zhang Chen , Han Xu , Li Chunhao , Leira Bernt Johan , Sævik Svein , Lu Dongzhe , Shi Wei , Li Xin
Published: November 2024

Operational modal analysis (OMA) is essential for long-term health monitoring of offshore wind turbines (OWTs), helping identifying changes in structural dynamic characteristics. OMA has been applied under parked or idle states for OWTs, assuming a linear and time-invariant dynamic system subjected to white noise excitations. The impact of complex operating environmental conditions on structural modal identification therefore requires systematic investigation. This paper studies the applicability of OMA based on covariance-driven stochastic subspace identification (SSI-COV) under various non-white noise excitations, using a DTU 10 MW jacket OWT model as a basis for a case study. Then, a scaled (1:75) 10 MW jacket OWT model test is used for the verification. For pure wave conditions, it is found that accurate identification for the first and second FA/SS modes can be achieved with significant wave energy. Under pure wind excitations, the unsteady servo control behavior leads to significant identification errors. The combined wind and wave actions further complicate the picture, leading to more scattered identification errors. The SSI-COV based modal identification method is suggested to be reliably applied for wind speeds larger than the rated speed and with sufficient wave energy. In addition, this method is found to perform better with larger misalignment of wind and wave directions. This study provides valuable insights in relation to the engineering applications of in situ modal identification techniques under operating conditions in real OWT projects.
The Contribution of Siliceous Plankton to Vertical Export Flux in the Eastern Mediterranean: A Comparative Study of the North Aegean, Cretan, and Ionian Seas
Authors: Nikolopoulou Ioanna , Skampa Elisavet , Varkitzi Ioanna , Dimiza Margarita D. , Parinos Constantine , Kambouri Georgia , Stavrakaki Ioanna , Gogou Alexandra , Triantaphyllou Maria V.
Published: November 2024

This study investigates the intricate dynamics of siliceous plankton species within the open marine regions of the Greek Seas, focusing on their seasonal and spatial variability. For this purpose, vertical export fluxes of diatoms (DtF), silicoflagellates (SF), and radiolaria (RF) were analyzed in three sediment trap time series obtained from the North Aegean, Cretan, and Ionian Seas. Special attention was given to diatom assemblages, resulting in the estimation of the DtF community structure and diversity for each studied site. Diatom flux values reached 353.9 × 103 valves m−2 day−1, 77.7 × 103 valves m−2 day−1, and 42.4 × 103 valves m−2 day−1 in the North Aegean, Ionian, and Cretan Seas, respectively. SF maxima were 1309.8 × 103 skeletons m−2 day−1 in the North Aegean Sea, 35.2 × 103 skeletons m−2 day−1 in the Ionian Sea, and 11.9 × 103 skeletons m−2 day−1 in the Cretan Sea (South Aegean Sea). RF values reached 13.9 × 103 radiolaria m−2 day−1, 11.9 × 103 radiolaria m−2 day−1, and 5.4 × 103 radiolaria m−2 day−1 in the North Aegean, Ionian, and Cretan Seas, respectively. The North Aegean Sea exhibited significantly higher mean total fluxes, particularly for diatoms, driven by the north-to-south oligotrophy gradient, which was influenced by riverine inflows and the nutrient-rich Black Sea water. In the Cretan and Ionian Seas, convective mixing and atmospheric deposition, especially during increased rainfall (precipitation) events, were identified as primary drivers for the increased siliceous plankton fluxes recorded in the late winter–spring months. Diatom communities were dominated by Naviculales and Fragilariales; the prevalence of the former in the North Aegean Sea is likely linked to the higher nutrient levels in its upper photic zone, as Naviculales includes species with a high affiliation to nutrient enrichment.
Ecosystem Structure and Function in the Sea Area of Zhongjieshan Islands Based on Ecopath Model
Authors: Qu Yao , Wang Zhongming , Zhou Yongdong , Liang Jun , Xu Kaida , Zhang Yazhou , Li Zhenhua , Dai Qian , Zhang Qiuhong , Jiang Yongsheng
Published: November 2024

Based on the field survey and reference data of the sea area of the Zhongjieshan Islands from 2021 to 2022, the Ecopath model was used to analyze the energy flow structure of the marine ecosystem of the sea area of the Zhongjieshan Islands; the energy structure of the marine ecosystem was divided into 21 functional groups, and its nutrient structure, energy flow, and total system characteristics were analyzed. The results show that the credibility of the model is 0.414, which is at a medium level. The trophic level of each functional group of the ecosystem in the sea area of Zhongjieshan Islands was 1–3.48, the energy flow structure of the system was mainly concentrated in the first five grades, and the trophic level was relatively simple, with the average energy transfer efficiency of the system being 8.11%, the energy flow range being 2.81–13.04%, the energy transfer efficiency of the primary producers of the system being 7.25%, and the energy conversion efficiency of the system debris being 9.12%. The total system throughput was 2125.96 t·km−2; The analysis of the overall characteristics of the ecosystem showed that the system connectance index and the system omnivory index were 0.45 and 0.24, respectively, while the Finn’s cycling index was 8.24, the Finn’s mean path length of the system was 2.72, and the total primary production/total respiration was 1.71. In this study, the marine ecosystem model of the sea area of the Zhongjieshan Islands was studied to understand the trophic structure and ecosystem status of the sea area, which is conducive to the sustainable utilization and scientific management of fishery resources in the sea area.
Dynamics of Irrigated Land Expansion in the Ouémé River Basin Using Field and Remote Sensing Data in the Google Earth Engine
Authors: Ahoton David Houéwanou , Bacharou Taofic , Bossa Aymar Yaovi , Sintondji Luc Ollivier , Bonkoungou Benjamin , Alofa Voltaire Midakpo
Published: November 2024

The availability of reliable and quantified information on the spatiotemporal distribution of irrigated land at the river basin scale is an essential step towards sustainable management of water resources. This research aims to assess the spatiotemporal extent of irrigated land in the Ouémé River basin using Landsat multi-temporal images and ground truth data. A methodology was built around the use of supervised classification and the application of an algorithm based on the logical expression and thresholding of a combination of surface temperature (Ts) and normalized difference vegetation index (NDVI). The findings of the supervised classification showed that agricultural areas were 16,003 km2, 19,732 km2, and 22,850 km2 for the years 2014, 2018, and 2022, respectively. The irrigated land areas were 755 km2, 1143 km2, and 1883 km2 for the same years, respectively. A significant increase in irrigated areas was recorded throughout the study period. The overall accuracy values of 79%, 82%, and 83% obtained during validation of the irrigated land maps indicate a good performance of the algorithm. The results suggest a promising application of the algorithm to obtain up-to-date information on the distribution of irrigated land in several regions of Africa.
Analyzing the Supply and Demand Dynamics of Urban Green Spaces Across Diverse Transportation Modes: A Case Study of Hefei City’s Built-Up Area
Authors: Gu Kang , Liu Jiamei , Wang Di , Dai Yue , Li Xueyan
Published: November 2024

With the increasing demands of urban populations, achieving a balance between the supply and demand in the spatial allocation of urban green park spaces (UGSs) is essential for effective urban planning and improving residents’ quality of life. The study of UGS supply and demand balance has become a research hotspot. However, existing studies of UGS supply and demand balance rarely simultaneously improve the supply side, demand side, and transportation methods that connect the two, nor do they conduct a comprehensive, multi-dimensional supply and demand evaluation. Therefore, this study evaluates the accessibility of UGS within Hefei’s built-up areas, focusing on age-specific demands for UGS and incorporating various travel modes, including walking, cycling, driving, and public transportation. An improved two-step floating-catchment area (2SFCA) method is applied to evaluate the accessibility of UGS in Hefei’s built-up areas. This evaluation combines assessments using the Gini coefficient, Lorenz curve, location entropy, and local spatial autocorrelation analysis, utilizing the ArcGIS 10.8 and GeoDa 2.1 platforms. Together, these methods enable a supply–demand balance analysis of UGSs to identify areas needing improvement and propose corresponding strategies. The research results indicate the following: (1) from a regional perspective, there are significant disparities in the accessibility of UGS within Hefei’s urban center, with the old city showing more imbalance than the new city. Areas with high demand and low supply are primarily concentrated in the old city, which require future improvement; (2) in terms of travel modes, higher-speed travel (such as driving) offers better and more equitable accessibility compared to slower modes (such as walking), highlighting transportation as a critical factor influencing accessibility; (3) regarding population demand, there is an overall balance in the supply of UGS, with local imbalances observed in the needs of residents across different age groups. Due to the high specific demand for UGS among older people and children, the supply and demand levels in these two age groups are more consistent. This study offers valuable insights for achieving the balanced, efficient, and sustainable development of the social benefits of UGS.
Local Sustainability Assessment of the Wonogiri Multipurpose Reservoir Catchment Area in Central Java Province, Indonesia
Authors: Rendrarpoetri Bunga Ludmila , Rustadi Ernan , Fauzi Akhmad , Pravitasari Andrea Emma
Published: November 2024

The sustainability of watershed management is a crucial issue that must be addressed to guarantee the persistence of watershed services including agriculture, food production, and energy supply. This issue has also been addressed in Presidential Regulation No. 18/2020 concerning the National Medium-Term Development Plans for 2020–2024, which stipulate the restoration of priority watersheds, including the Upstream Bengawan Solo Watershed. This study seeks to address this information gap by assessing the local sustainability of the watershed from a temporal dynamics perspective by calculating the Local Sustainability Index (LSI), Local Moran Index, and spatial associations. Measuring sustainable development indices locally is essential because each location has different characteristics, and using specific indicators at the local level is rarely done. The enactment of the national law on village autonomy in Indonesia necessitates the formulation of sustainable development indicators at the village level. These indicators serve as the metrics and frameworks for local government policies and initiatives. Our results show that village sustainability in the social and economic dimensions has increased from 2007 to 2021, especially in urban activity center areas that serve social and economic facilities. This seems different in the environmental dimension, where the sustainability value decreased from 2007 to 2021. The concentration of low sustainability values on ecological conditions occurred in pocket areas. Environmental problems were indicated by land-use conversion and disaster areas.