bluuebunny/crossref_metadata_2025 huggingface dataset:
Laboratory study of plugging mechanism and seal integrity in fractured formations using a new blend of lost circulation materials
Authors: Elahifar Behzad , Hosseini Erfan
Published: January 2023

The problem of lost circulation occurred long during the drilling operation. Through induced and natural fractures, huge drilling fluid losses lead to higher operating expenses during the drilling. Historically, this problem was addressed with the help of the Lost Circulation Materials (LCMs). These materials are added to the drilling fluid to seal the fractures and increase fracture initiation or propagation pressure. Therefore, understanding the mechanisms of fracture sealing and the performance of the lost circulation materials is critical if the problem of lost circulation is to be mitigated effectively. Despite extensive advances in the last couple of decades, lost circulation materials used today still have disadvantages, such as damaging production zones, failing to seal large fractures, or plugging drilling tools. Here, we propose a new blend of smart expandable lost circulation material (LCM) to remotely control the expanding force and functionality of the injected LCM. This paper aimed to assess the performance of the selected LCMs (Mica, Wheat Straw, Oak Shell, and Sugarcane Bagasse Fiber or Canes) in water-based drilling fluids. The particle bridging of LCMs was investigated using particle bridging experiments in the laboratory. Moreover, we determined the particle size distribution of D50. The cell utilized in the sealing experiments had 1000- and 3000 micron fractures to mimic different size fractures in the formation. Fracture widths are predicted based on well-log data and adaptation of existing models in the desired oil field. The concentrations of LCMs in Mica, Wheat Straw, Oak Shell, and Sugarcane Bagasse Fiber (Canes) were (25, 50, and 80 ppb), (1.5, 2, 2.5 ppb), (3, 6, and 10 ppb), and (1.5, 2, 2.5 ppb), respectively. The results indicate that a combination of LCMs outperforms individual LCMs. When used individually, Oak Shells performed the highest, followed by Mica and Sugarcane Bagasse Fiber mixtures. Also, the Wheat Straw blend served the weakest lost circulation treatments. Finally, the combination applied in this investigation successfully sealed fractures up to 3 mm in diameter in the targeted oil field, which traditional LCM would be unable to do. Due to the abundance and low cost of these materials in the study area, they can be used to ensure successful plugging.

Graphical Abstract
Comprehensive use of the Gutenberg–Richter law and geotomography for improving seismic hazard evaluation in hard coal mines
Authors: Dubiński Józef , Stec Krystyna , Krupanek Jacek
Published: January 2023

Mining-induced seismicity occurs in numerous underground mines worldwide where extraction is conducted at great depths or in areas characterised by complex tectonic structure. It is accompanied by rock bursts, which result in the loss of working functionality and the possibility of accidents among personnel. The issue of a constant and reliable seismic hazard evaluation is of key significance for both the safety of miners and the stability of production. Research on its improvement is directed at developing new interpretive solutions and methods. The nature of the presented solution is the complex interpretation of seismological data that characterise rock mass seismicity and of underground measurement results in the form of a map presenting the longitudinal wave propagation velocity distribution in the rock surrounding the mined coal seam. The solution was tested in hard coal mines located in the Upper Silesian Coal Basin. The mines are equipped with a modern seismological system enabling the constant monitoring of seismicity together with hazard level evaluation as well as with seismic apparatus for conducting periodic measurements of the seismic wave propagation velocity before the mining face. Comprehensive seismic hazard evaluation criteria were determined based on the obtained results, involving the anomaly of the Gutenberg–Richter law “b” value and the maximum longitudinal seismic wave propagation velocity in the roof rock. The obtained experience and the result validation of this new comprehensive hazard evaluation method confirm its practical usefulness and indicate the directions of improvement for the solution in question.
Impact of lead on seed germination, seedling growth, chemical composition, and forage quality of different varieties of Sorghum
Authors: Osman Hanan E. , Fadhlallah Ruwaydah S.
Published: January 2023

Due to heavy metal contamination, there are currently significant losses in agriculture and food security. Anthropogenic sources led to increased heavy metal contamination, which was becoming an attentive problem for agriculture and food safety. Among these metals, lead (Pb), which is toxic and affects crop production, has emerged as a standout due to its severe toxicity and relatively obscure mode of action.This study aims to compare the chemical composition and nutritional value of three tested sorghum cultivars grown in Pb-spiked soil with different levels of Pb (0, 100, 200, 400 and 800 mg kg−1). Also, the impact of different Pb levels on germination, fresh and dry weight of underground and aboveground parts, and morphological characters of three tested sorghum cultivars were assessed. The results emphasized that the germination index (GRI), radical and plumule length (cm), vigor index (VI), and tolerance index (TI) declined significantly by increasing Pb levels at all the examined cultivars. The morphological parameters of the examined cultivars decreased progressively with increasing Pb concentration for three tested sorghum cultivars. The nutritive values and forage quality of examined cultivars are negatively impacted by higher Pb levels, rendering them hazardous to animal consumption. These results may help scheme a mitigation strategy for lead injuriousness.
Veracruz and the Caribbean in the Seventeenth Century
Authors: Clark Joseph M. H.
Published: January 2023

In the seventeenth century, Veracruz was the busiest port in the wealthiest colony in the Americas. People and goods from five continents converged in the city, inserting it firmly into the early modern world's largest global networks. Nevertheless, Veracruz never attained the fame or status of other Atlantic ports. Veracruz and the Caribbean in the Seventeenth Century is the first English-language, book-length study of early modern Veracruz. Weaving elements of environmental, social, and cultural history, it examines both Veracruz's internal dynamics and its external relationships. Chief among Veracruz's relationships were its close ties within the Caribbean. Emphasizing relationships of small-scale trade and migration between Veracruz and Caribbean cities like Havana, Santo Domingo, and Cartagena, Veracruz and the Caribbean shows how the city's residents – especially its large African and Afro-descended communities – were able to form communities and define identities separate from those available in the Mexican mainland.
Trajectories of Authoritarianism in Rwanda
Authors: Desrosiers Marie-Eve
Published: January 2023

Challenging assumptions regarding the strength and control of authoritarian governments in Rwanda in the decades before the 1994 genocide, Marie-Eve Desrosiers uses original archival data and interviews to highlight the complex relations between authorities, opponents, and society. Through careful, detailed analysis Desrosiers offers a nuanced assessment of the functions and evolution of authoritarianism over time, demonstrating how the governments of Rwanda's first two post-independence Republics (1962–1990) sought and often struggled to cement their rule. Whilst the deeper, lived realities of authoritarianism are generally neglected by multi-cases comparisons at the heart of comparative authoritarian studies, this illuminating survey highlights the essential, yet subtle authoritarian strategies, patterns, and forms of decay that are too often overlooked when addressing authoritarian contexts.
Dynamics of flexible filaments in oscillatory shear flows
Authors: Bonacci Francesco , Chakrabarti Brato , Saintillan David , du Roure Olivia , Lindner Anke
Published: January 2023

The fluid–structure interactions between flexible fibres and viscous flows play an essential role in various biological phenomena, medical problems and industrial processes. Of particular interest is the case of particles transported freely in time-dependent flows. This work elucidates the dynamics and morphologies of actin filaments under oscillatory shear flows by combining microfluidic experiments, numerical simulations and theoretical modelling. Our work reveals that, in contrast to steady shear flows, in which small orientational fluctuations from a flow-aligned state initiate tumbling and deformations, the periodic flow reversal allows the filament to explore many different configurations at the beginning of each cycle. Investigation of filament motion during half time periods of oscillation highlights the critical role of the initial filament orientation on the emergent dynamics. This strong coupling between orientation and deformation results in new deformation regimes and novel higher-order buckling modes absent in steady shear flows. The primary outcome of our analysis is the possibility of suppression of buckling instabilities for certain combinations of the oscillation frequency and initial filament orientation, even in very strong flows. We explain this unusual behaviour through a weakly nonlinear Landau theory of buckling, in which we treat the filaments as inextensible Brownian Euler–Bernoulli rods whose hydrodynamics is described by local slender-body theory.
Citizenship, Identity, and Veiling: Interrogating the Limits of Article 8 of the European Convention on Human Rights in Cases Involving the Religious Dress of Muslim Women
Authors: Costello Róisín Áine , Ahmed Sahar
Published: January 2023

In 2021, the debate about the spaces in which Europe’s Muslim citizens should be permitted to wear religious veils was reanimated by the introduction of new prohibitions introduced in Switzerland and France, and the decision of the Court of Justice of the European Union in joined cases C-804/18 and C-341/19. This article examines the jurisprudence of the European Court of Human Rights concerning veiling. We argue that veil bans reduce the ability of Muslim women to actualize themselves as citizens by limiting their capacity to develop their identity through autonomous action. As such, we argue, the right ultimately at stake—which should protect rights in respect of veiling—is the right to a private life under Article 8 of the European Convention on Human Rights, and judicial and popular conceptions of veiling should be reoriented to accommodate this view. Doing so, we argue, highlights the full range of functions that veiling implicates—including religious but also secular identarian concerns and exposes how a usually expansive right has been curtailed in cases involving veiling.
Showing commitment or incompetence? When and how newcomers' information seeking elevates (degrades) task-related outcomes
Authors: Deng Hui , Zhang Yihua , Wu Shaoxue , Wu Wenbing , Ni Dan , Zhang Xiaoyan
Published: January 2023

To date, empirical research exploring the complex mechanisms of when and how information seeking from supervisor affects newcomers' task-related outcomes remains in its infancy. With a sample of 394 newcomers and their supervisors, drawing on the conservation of resources theory and professional image construction theory, we proposed and confirmed two paradoxical paths regarding perceptions of professional image construction – concern about impairing competence-image and confidence in improving commitment-image – that connect newcomers' information seeking from supervisor with their emotional exhaustion during socialization and ultimately can elevate and degrade their task-related outcomes, respectively. In addition, we found that supervisors' favorable feedback weakened the relationship between information seeking from supervisor and competence-image impairment concern, whereas it strengthened the linkage between information seeking and commitment-image improvement confidence. This study thus provides a more comprehensive picture for scholars and practitioners.
New paromomyids (Mammalia, Primates) from the Paleocene of southwestern Alberta, Canada, and an analysis of paromomyid interrelationships
Authors: Scott Craig S. , López-Torres Sergi , Silcox Mary T. , Fox Richard C.
Published: January 2023

Paromomyidae are one of several families of plesiadapiforms that flourished during the Paleocene in North America soon after the extinction of non-avian dinosaurs some 66 million years ago. Although they are often among the best-represented plesiadapiforms in mammalian faunas in both North America and Europe, the early history of paromomyids is poorly understood, and their fossil record at higher latitudes is comparatively depauperate. We report here on the discovery of two new species of paromomyids from Paleocene deposits in southwestern Alberta: Edworthia greggi new species is the second known species of the basal paromomyid Edworthia Fox, Scott, and Rankin, 2010 whereas Ignacius glenbowensis new species is among the most abundantly represented species of Ignacius Matthew and Granger, 1921. These new discoveries document, for the first time, parts of the upper dentition of Edworthia, and the new species of Ignacius represents the first new, pre-Clarkforkian species of the genus to be described in nearly 100 years. A comprehensive phylogenetic analysis of nearly all known paromomyid taxa (including the new species described herein) recovered both species of Edworthia near the base of the paromomyid tree in a polytomy with Paromomys depressidens Gidley, 1923 and a paraphyletic Ignacius. The new paromomyids from Alberta not only increase the known taxonomic diversity of Edworthia and Ignacius but also add significantly to knowledge of the dental anatomy of these poorly known genera and further add to a uniquely Canadian complement of Paleocene plesiadapiforms.

UUID: http://zoobank.org/a2f31cfe-09b9-4168-b09f-9d0c3d0af342
Taxonomy and paleobiogeography of rudist bivalves from Upper Cretaceous strata, Gulf Coastal Plain and Puerto Rico, USA
Authors: Zimmerman Alexander N. , Johnson Claudia C. , Phillips George E. , Ehret Dana J.
Published: January 2023

This study provides the first focused investigation of rudist bivalves from the Upper Cretaceous of the Gulf Coastal Plain (GCP) in the southern US and previously undescribed specimens from the Flor de Alba Limestone Member of the Pozas Formation in Puerto Rico. Identified rudists from the GCP comprise the Monopleuridae, includingGyropleura, as well as Radiolitidae, includingBiradiolites cardenasensi,Durania maxima,Guanacastea jamaicensis,Radiolites acutocostata, andSauvagesia. Integrating rudist occurrences within well-established GCP biostratigraphy allows for extension of upper ranges ofD.maximaandR.acutocostatainto the late Campanian, and extension of the lower ranges ofB.cardenasensisandG.jamaicensisinto the early Campanian. Identified rudists from Puerto Rico comprise the Hippuritidae and includeBarrettia monilifera, which supports the age of the Flor de Alba Limestone Member of the Pozas Formation as middle Campanian. Combined taxonomic, biostratigraphic, and paleobiogeographic analyses indicate there is no rudist fauna endemic to the GCP, and the region marks the northeastern range of the Caribbean generaBiradiolites,Durania,Guanacastea,Gyropleura,Radiolites,andSauvagesiaduring the Campanian and Maastrichtian. The new occurrences help inform future updates of Late Cretaceous sea surface-current reconstructions for the Caribbean and Western Interior Seaway, USA.
Spatial heterogeneity in benthic foraminiferal assemblages tracks regional impacts of paleoenvironmental change across Cretaceous OAE2
Authors: Bryant Raquel , Belanger Christina L.
Published: January 2023

The impact of global climate events on local ecosystems can vary spatially. Understanding this potential heterogeneity can illuminate which environments will be most impacted and the proximal drivers of ecosystem responses. Cenomanian–Turonian marine deposits of the Western Interior Seaway (WIS) record paleoceanographic changes associated with the Greenhorn transgression and the onset of Oceanic Anoxic Event 2 (OAE2). They provide an ideal setting to study basin-wide paleoecological responses during a global perturbation. Here, we integrate benthic foraminiferal assemblages from before, during, and after OAE2 via multivariate ordination analysis to examine spatial patterns in faunal responses across the western United States on a common scale and to interrogate a previously defined faunal marker commonly used for basin-wide correlation, the Benthonic Zone (BZ). We identify oxygenation and organic matter quality as primary and secondary controls of faunal variation across the 10 stratigraphic records and use this variation to infer paleoenvironmental changes. Stratigraphic trends reveal, in contrast to previous studies, deoxygenation at the onset of OAE2. They also reveal temporal patterns in oxygenation and productivity consistent with the gradual northward migration of a southern water mass into the WIS. This spatial heterogeneity hinders the use of the BZ as a temporal marker, because assemblages change in response to diachronous environmental change, and thus timing of the BZ with respect to OAE2 is not consistent across the basin. Our study demonstrates that regional processes can overshadow ecosystem responses to global events and underscores the importance of considering how changes in the position of water masses impact the expression of global biogeochemical perturbations.
Outcome-based contracting and gaming practices in marketised public employment services. Dilemmas from the Italian case
Authors: Scarano Gianluca
Published: January 2023

Concerns about the unintended effects of marketised public employment services are increasingly expressed because there is mounting evidence that such services are frequently characterised by various gaming practices on the part of their providers. To prevent these unintended consequences, payment-by-result approaches have been progressively strengthened.

The aim of the research reported in this article was to investigate the extent to which such approaches are able to make service providers accountable for client outcomes. The study used two Italian regional cases in order to compare different and alternative contracting arrangements: Lombardy (outcome-based payments), and Emilia-Romagna (fixed payments). Drawing on rich administrative databases, the analysis relied on a quantitative methodology based on propensity score matching and logistic regressions. Even if outcome-based contracting can make service providers financially accountable for the service outcome, the results of the analysis show that it does not sufficiently prevent gaming practices, resulting in inequity among the services provided.
A large A(H3N2) influenza outbreak with a high attack rate in a drug user community in Italy, April 2022
Authors: Gori Maria , Fappani Clara , Bianchi Silvia , Canuti Marta , Colzani Daniela , Ottogalli Paolo , Duehren Sarah , Tanzi Elisabetta , Amendola Antonella , Boschini Antonio
Published: January 2023

Despite the COVID-19 pandemic, influenza remains an important issue. Especially in community settings, influenza outbreaks can be difficult to control and can result in high attack rates. In April 2022, a large A(H3N2) influenza outbreak spread in the largest Italian drug-rehabilitation community. One hundred eighty-four individuals presented influenza-like symptoms (attack rate of 26.2%); 56% previously received the influenza vaccine. Sequence analyses highlighted a genetic drift from the vaccine strain, which may have caused the observed lack of protection.
Patient and parent-reported outcomes in paediatric ventricular assist device support: a multi-center ACTION learning network feasibility and pilot experience
Authors: Cousino Melissa K. , May Lindsay J. , Smyth Lauren , McQueen Melissa , Thompson Katherine , Hunter Tiffany , Ventresco Courtney , Fields Katrina , Murray Jenna , Machado Desiree S. , Shezad Muhammad , Zafar Farhan , Rosenthal David N. , Lorts Angela , Blume Elizabeth D.
Published: January 2023

Background:

Patient- and proxy-reported outcomes (PROs) are an important indicator of healthcare quality and can be used to inform treatment. Despite the widescale use of PROs in adult cardiology, they are underutilised in paediatric cardiac care. This study describes a six-center feasibility and pilot experience implementing PROs in the paediatric and young adult ventricular assist device population.

Methods:

The Advanced Cardiac Therapies Improving Outcomes Network (ACTION) is a collaborative learning network comprised of 55 centres focused on improving clinical outcomes and the patient/family experience for children with heart failure and those supported by ventricular assist devices. The development of ACTION’s PRO programme via engagement with patient and parent stakeholders is described. Pilot feasibility, patient/parent and clinician feedback, and initial PRO findings of patients and families receiving paediatric ventricular assist support across six centres are detailed.

Results:

Thirty of the thirty-five eligible patients (85.7%) were enrolled in the PRO programme during the pilot study period. Clinicians and participating patients/parents reported positive experiences with the PRO pilot programme. The most common symptoms reported by patients/parents in the first month post-implant period included limitations in activities, dressing change distress, and post-operative pain. Poor sleep, dressing change distress, sadness, and fatigue were the most common symptoms endorsed >30 days post-implant. Parental sadness and worry were notable throughout the entirety of the post-implant experience.

Conclusions:

This multi-center ACTION learning network-based PRO programme demonstrated initial success in this six-center pilot study experience and yields important next steps for larger-scale PRO collection, research, and clinical intervention.
Evaluation of the accuracy of a six-degree-of-freedom robotic couch using optical surface and cone beam CT images of an SRS QA phantom
Authors: Zhang Q. , Chen Y. , Fang D. , Iannuzzi C. , Klein E.
Published: January 2023

Purpose:

To assess the accuracy of the Varian PerfectPitch six-degree-of-freedom (6DOF) robotic couch by using a Varian SRS QA phantom.

Methods:

The stereotactic radiosurgery (SRS) phantom has five tungsten carbide BBs each with 7·5 mm in diameter arranged with the known geometry. Optical surface images and cone beam CT (CBCT) images of the phantom were taken at different pitch, roll and rotation angles. The pitch, roll, and rotation angles were varied from −3 to 3 degrees by inputs from the linac console. A total of 39 Vision RT images with different rotation angle combinations were collected, and the Vision RT software was used to determine the rotation angles and translational shifts from those images. Eight CBCT images at most allowed rotational angles were analysed by in-house software. The software took the coordinates of the voxel of the maximum CT number inside a 7·5-mm sphere surrounding one BB to be the measured position of this BB. Expected BB positions at different rotation angles were determined by multiplying measured BB positions at zero pitch and roll values by a rotation matrix. Applying the rotation matrix to 5 BB positions yielded 15 equations. A linear least square method was used for regression analysis to approximate the solutions of those equations.

Results:

Of the eight calculations from CBCT images, the maximum rotation angle differences (degree) were 0·10 for pitch, 0·15 for roll and 0·09 for yaw. The maximum translation differences were 0·3 mm in the left–right direction, 0·5 mm in the anterior–posterior direction and 0·4 mm in the superior–inferior direction.

Conclusions:

The uncertainties of the 6-DOF couch were examined with the methods of optical surface imaging and CBCT imaging of the SRS QA phantom. The rotational errors were less than 0·2 degree, and the isocentre shifts were less than 0·8 mm.
Psychosocial well-being among patients with malignant pleural mesothelioma
Authors: Demirjian Caraline Craig , Saracino Rebecca M. , Napolitano Stephanie , Schofield Elizabeth , Walsh Leah E. , Key R. Garrett , Holland Jimmie
Published: January 2023

Objectives

The investigators conducted a psychosocial needs assessment of mesothelioma patients through self-report measures of quality of life (QOL), coping, depression, and social support.

Methods

Patients with malignant pleural mesothelioma (MPM) (N = 67) completed a battery of assessments at a single timepoint after being approached during routine medical oncology clinic appointments or by letter.

Results

Participants were predominately male (70.0%; n = 47) and ranged in age from 35 to 83 years old (M = 65.61, SD = 9.71). Most participants were white (88.0%; n = 59), and 10.0% (n = 7) were identified as Hispanic. The majority were married or living with a partner (93.0%; n = 62) and had some college or more education (64.0%; n = 43). Fourteen percent of participants (n = 11) endorsed significantly elevated depression symptoms. No significant demographic or clinical differences in depressed compared to nondepressed participants were observed, with a trend toward those identifying as Hispanic and those who were divorced as being more likely to be depressed. For the total sample, the most frequently endorsed coping strategies were active coping, emotional support, and acceptance.

Significance of results

The present study did not identify any clear correlates of depression or QOL among patients with MPM. This research contributes to the small literature on psychosocial functioning in patients with MPM and provides putative directions for future larger studies and the development of interventions to provide appropriate support to diverse patients with MPM.
Prenatal dietary patterns in relation to adolescent offspring adiposity and adipokines in a Mexico City cohort
Authors: Fossee Erica , Zamora Astrid N. , Peterson Karen E. , Cantoral Alejandra , Perng Wei , Téllez-Rojo Martha M. , Torres-Olascoaga Libni A. , Jansen Erica C.
Published: January 2023

Maternal diet during pregnancy has been associated with obesity among offspring. The extent to which trimester-specific dietary patterns are associated with markers of adiposity during adolescence remains unclear. We examined associations between prenatal diet patterns with adolescent offspring measures of adiposity and adipokines in 384 mother–adolescent dyads from the Mexico City ELEMENT cohort. Trimester-specific diet patterns were derived from principal component analysis of food frequency questionnaire data. Adolescent anthropometry and serum leptin and adiponectin were measured at 10–17 years. Three maternal diet patterns were identified: Prudent Diet (PD), high in fish and vegetables, the High Meat and Fat Diet (HMFD), high in pork and processed meats, and the Transitioning Mexican Diet (TMD), high in corn tortillas and sugar-sweetened beverages. Multiple linear regression was used to estimate sex-stratified associations among quartiles of diet patterns with adiposity and adipokines, adjusting for maternal marital status, education, and parity. First trimester TMD was associated with greater anthropometric measures and higher leptin in females, while third trimester HMFD was associated higher body fat percentage, triceps thickness, waist circumference, and leptin, but lower adiponectin among males. Contrary to expectation, there were positive associations between the trimester 1 PD pattern and anthropometric measurements in females, and for trimester 2 HMFD and TMD patterns with adipokines among males. Findings suggest maternal diet patterns may influence offspring adiposity markers during adolescence in a sex-specific manner.
No Correlation Between Mood or Motivation and the Processing of Global and Local Information
Authors: De Luca Alberto , Verschoor Stephan , Hommel Bernhard
Published: January 2023

Abstract. Mood has been argued to impact the breadth of human attention, but the empirical evidence supporting this claim remains shaky. Gable and Harmon-Jones (2008) have attributed previous empirical inconsistencies regarding the effect of mood on attentional breath to a critical role of approach/avoidance motivation. They demonstrated that the combination of positive affect with high, but not with low, motivational intensity improves performance during processing local information and impairs performance during processing global information. The latter, but not the former, was replicated by Domachowska et al. (2016). Since we were interested in the modulation of attention by valence and motivation, and considering the inconsistencies in the findings, we replicated the critical experiments of both studies in four online experiments but found no significant effect of either valence or motivational intensity on attention. Taken together, our evidence casts doubt on a systematic relationship between mood or motivation on the one hand and global/local processing on the other.
The Impact of Depth of Encoding on the Transfer of Test Enhanced Learning
Authors: DiMarco Donnelle , Marmurek Harvey
Published: January 2023

Abstract. The mediator effectiveness hypothesis states the benefit of retrieval practice is a consequence of the activation of mediators linking cue and target items during review. Evidence has found that mediators are more effective at prompting recall of target words than words not associated with the original cue, a pattern that is larger following testing than restudy. The benefit of testing for unstudied cues at the final test is referred to as transfer of test-enhanced learning. The current study examined whether the activation of mediators is moderated by the depth of processing completed at encoding. During an initial study of weakly related word pairs (e.g., Mother–CHILD), participants completed an encoding task that directed deep, shallow, or no specific depth of processing. During review, participants restudied the pairs or attempted to recall the target given the original cue (e.g., Mother). On the final test, participants were presented with unstudied cues that were related to either the original cue (mediators, e.g., Father) or the target (target-related cues, e.g., Baby). The results found mediator generation during review to be greatly impaired by shallow processing tasks completed during encoding. In contrast, the effectiveness of target-related cues was not affected by depth of processing tasks.
Social Support in Online Peer Groups for Celiac	Disease
Authors: Köstlin Dorothee , Siem Birte , Rohmann Anette
Published: January 2023

Abstract: Background: While there is considerable evidence for the positive effects of onsite peer support on health outcomes, few studies have addressed the impact of online peer support. Aims: This study differentiates between emotional (ES) and informational (IS) online peer support for people with celiac disease. We hypothesize that receiving ES and IS positively correlates with self-reported well-being and dietary compliance and that these relations would be moderated by the duration of illness. Method: We used a correlational, cross-sectional design ( N = 369). Results: Both functions of support were positively related to well-being and dietary compliance. The strengths of the positive relationships between ES and both outcomes and IS and well-being were independent of the duration of illness. IS was significantly positively related to dietary compliance among participants with a longer duration of illness, but unrelated among participants with a shorter duration. Limitations: Due to the correlational design, we cannot infer causality. Conclusion: Our findings advance the understanding of online support in contexts where proper self-management is crucial for health outcomes.
Climate Drivers of Malaria Transmission Seasonality and Their Relative Importance in Sub‐Saharan Africa
Authors: Yamba Edmund I. , Fink Andreas H. , Badu Kingsley , Asare Ernest O. , Tompkins Adrian M. , Amekudzi Leonard K.
Published: January 2023

A new database of the Entomological Inoculation Rate (EIR) was used to directly link the risk of infectious mosquito bites to climate in Sub‐Saharan Africa. Applying a statistical mixed model framework to high‐quality monthly EIR measurements collected from field campaigns in Sub‐Saharan Africa, we analyzed the impact of rainfall and temperature seasonality on EIR seasonality and determined important climate drivers of malaria seasonality across varied climate settings in the region. We observed that seasonal malaria transmission was within a temperature window of 15°C–40°C and was sustained if average temperature was well above 15°C or below 40°C. Monthly maximum rainfall for seasonal malaria transmission did not exceed 600 in west Central Africa, and 400 mm in the Sahel, Guinea Savannah, and East Africa. Based on a multi‐regression model approach, rainfall and temperature seasonality were found to be significantly associated with malaria seasonality in all parts of Sub‐Saharan Africa except in west Central Africa. Topography was found to have significant influence on which climate variable is an important determinant of malaria seasonality in East Africa. Seasonal malaria transmission onset lags behind rainfall only at markedly seasonal rainfall areas such as Sahel and East Africa; elsewhere, malaria transmission is year‐round. High‐quality EIR measurements can usefully supplement established metrics for seasonal malaria. The study's outcome is important for the improvement and validation of weather‐driven dynamical mathematical malaria models that directly simulate EIR. Our results can contribute to the development of fit‐for‐purpose weather‐driven malaria models to support health decision‐making in the fight to control or eliminate malaria in Sub‐Saharan Africa.
Using the Shallow Strain Tensor to Characterize Deep Geologic Reservoirs
Authors: Murdoch Lawrence C. , DeWolf Scott , Germanovich Leonid N. , Moysey Stephen , Hanna Alex , Roudini Soheil , Moak Robert
Published: January 2023

Storing and recovering water, carbon, and heat from geologic reservoirs is central to managing resources in a changing climate. We tested the hypothesis that the strain tensor caused by injecting or producing fluids can be measured at shallow depths and interpreted to advance understanding of underlying deep aquifers or reservoirs. Geodetic‐grade strainmeters were deployed at 30 m depth overlying the Bartlesville Formation, a 500‐m‐deep sandstone near Tulsa, OK. The strainmeters are 220 m east of injection well 9A completed in a permeable lens at the base of the Bartlesville Formation. Water was injected into well 9A at approximately 1.0 L/s during four tests that ranged in duration from a few hours to a few weeks. The horizontal strain increased (tension) and the circumferential strain was a few times larger than the radial strain. The vertical strain decreased (compression) during injection. Strain rates were approximately 100 nε/day during the first few hours, but the rates decreased and were approximately 10 nε/day during most of the tests. Four independent methods of poroelastic simulation and inversion predict reservoir properties and geometries that are similar to each other and consistent with independent information about the reservoir. All strain interpretations predict that a boundary to the permeable lens occurs beneath the vicinity of the strainmeters, which is consistent with core data from the site. The boundary of the permeable lens is located by matching the vertical, radial and circumferential strains, which demonstrates the value of measuring the strain tensor.
Impact of AMPK on cervical carcinoma progression and metastasis
Authors: Konieczny Paweł , Adamus Tomasz , Sułkowski Maciej , Skrzypek Klaudia , Majka Marcin
Published: January 2023

Cervical cancer (CC) is the fourth most common malignant neoplasm among women. Late diagnosis is directly associated with the incidence of metastatic disease and remarkably limits the effectiveness of conventional anticancer therapies at the advanced tumor stage. In this study, we investigated the role of 5’AMP-activated kinase (AMPK) in the metastatic progression of cervical cancer. Since the epithelial mesenchymal transition (EMT) is known as major mechanism enabling cancer cell metastasis, cell lines, which accurately represent this process, have been used as a research model. We used C-4I and HTB-35 cervical cancer cell lines representing distant stages of the disease, in which we genetically modified the expression of the AMPK catalytic subunit α. We have shown that tumor progression leads to metabolic deregulation which results in reduced expression and activity of AMPK. We also demonstrated that AMPK is related to the ability of cells to acquire invasive phenotype and potential for in vivo metastases, and its activity may inhibit these processes. Our findings support the hypothesis that AMPK is a promising therapeutic target and modulation of its expression and activity may improve the efficacy of cervical cancer treatment.
Dual inhibition of thioredoxin reductase and proteasome is required for auranofin-induced paraptosis in breast cancer cells
Authors: Seo Min Ji , Kim In Young , Lee Dong Min , Park Yeon Jung , Cho Mi-Young , Jin Hyo Joon , Choi Kyeong Sook
Published: January 2023

Auranofin (AF), a gold (I)-containing phosphine compound, is being investigated for oncological application as a repurposed drug. We show here that 4~5 µM AF induces paraptosis, a non-apoptotic cell death mode characterized by dilation of the endoplasmic reticulum (ER) and mitochondria, in breast cancer cells. Although the covalent inhibition of thioredoxin reductase (TrxR), an enzyme that critically controls intracellular redox homeostasis, is considered the primary mechanism of AF’s anticancer activity, knockdown of TrxR1 did not induce paraptosis. Instead, both TrxR1 knockdown plus the proteasome inhibitor (PI), bortezomib (Bz), and 2 μM AF plus Bz induced paraptosis, thereby mimicking the effect of 5 μM AF. These results suggest that the paraptosis induced by 5 μM AF requires the inhibition of both TrxR1 and proteasome. We found that TrxR1 knockdown/Bz or subtoxic doses of AF and Bz induced paraptosis selectively in breast cancer cells, sparing non-transformed MCF10A cells, whereas 4~5 μM AF killed both cancer and MCF10A cells. GSH depletion was found to be more critical than ROS generation for the paraptosis induced by dual TrxR1/proteasome inhibition. In this process, the ATF4/CHAC1 (glutathione-specific gamma-glutamylcyclotransferase 1) axis leads to GSH degradation, contributing to proteotoxic stress possibly due to the accumulation of misfolded thiol-containing proteins. These results suggest that the paraptosis-inducing strategy of AF plus a PI may provide an effective therapeutic strategy against pro-apoptotic therapy-resistant cancers and reduce the potential side effects associated with high-dose AF.
PRMT5-mediated regulatory arginine methylation of RIPK3
Authors: Chauhan Chanchal , Martinez-Val Ana , Niedenthal Rainer , Olsen Jesper Velgaard , Kotlyarov Alexey , Bekker-Jensen Simon , Gaestel Matthias , Menon Manoj B.
Published: January 2023

The TNF receptor-interacting protein kinases (RIPK)-1 and 3 are regulators of extrinsic cell death response pathways, where RIPK1 makes the cell survival or death decisions by associating with distinct complexes mediating survival signaling, caspase activation or RIPK3-dependent necroptotic cell death in a context-dependent manner. Using a mass spectrometry-based screen to find new components of the ripoptosome/necrosome, we discovered the protein-arginine methyltransferase (PRMT)-5 as a direct interaction partner of RIPK1. Interestingly, RIPK3 but not RIPK1 was then found to be a target of PRMT5-mediated symmetric arginine dimethylation. A conserved arginine residue in RIPK3 (R486 in human, R415 in mouse) was identified as the evolutionarily conserved target for PRMT5-mediated symmetric dimethylation and the mutations R486A and R486K in human RIPK3 almost completely abrogated its methylation. Rescue experiments using these non-methylatable mutants of RIPK3 demonstrated PRMT5-mediated RIPK3 methylation to act as an efficient mechanism of RIPK3-mediated feedback control on RIPK1 activity and function. Therefore, this study reveals PRMT5-mediated RIPK3 methylation as a novel modulator of RIPK1-dependent signaling.
Provably-secure quantum randomness expansion with uncharacterised homodyne detection
Authors: Wang Chao , Primaatmaja Ignatius William , Ng Hong Jie , Haw Jing Yan , Ho Raymond , Zhang Jianran , Zhang Gong , Lim Charles
Published: January 2023

Quantum random number generators (QRNGs) are able to generate numbers that are certifiably random, even to an agent who holds some side information. Such systems typically require that the elements being used are precisely calibrated and validly certified for a credible security analysis. However, this can be experimentally challenging and result in potential side-channels which could compromise the security of the QRNG. In this work, we propose, design and experimentally demonstrate a QRNG protocol that completely removes the calibration requirement for the measurement device. Moreover, our protocol is secure against quantum side information. We also take into account the finite-size effects and remove the independent and identically distributed requirement for the measurement side. More importantly, our QRNG scheme features a simple implementation which uses only standard optical components and are readily implementable on integrated-photonic platforms. To validate the feasibility and practicability of the protocol, we set up a fibre-optical experimental system with a home-made homodyne detector with an effective efficiency of 91.7% at 1550 nm. The system works at a rate of 2.5 MHz, and obtains a net randomness expansion rate of 4.98 kbits/s at 1010 rounds. Our results pave the way for an integrated QRNG with self-testing feature and provable security.
Large scale phenotype imputation and in vivo functional validation implicate ADAMTS14 as an adiposity gene
Authors: Kentistou Katherine A. , Luan Jian’an , Wittemans Laura B. L. , Hambly Catherine , Klaric Lucija , Kutalik Zoltán , Speakman John R. , Wareham Nicholas J. , Kendall Timothy J. , Langenberg Claudia , Wilson James F. , Joshi Peter K. , Morton Nicholas M.
Published: January 2023

Obesity remains an unmet global health burden. Detrimental anatomical distribution of body fat is a major driver of obesity-mediated mortality risk and is demonstrably heritable. However, our understanding of the full genetic contribution to human adiposity is incomplete, as few studies measure adiposity directly. To address this, we impute whole-body imaging adiposity phenotypes in UK Biobank from the 4,366 directly measured participants onto the rest of the cohort, greatly increasing our discovery power. Using these imputed phenotypes in 392,535 participants yielded hundreds of genome-wide significant associations, six of which replicate in independent cohorts. The leading causal gene candidate, ADAMTS14, is further investigated in a mouse knockout model. Concordant with the human association data, the Adamts14−/− mice exhibit reduced adiposity and weight-gain under obesogenic conditions, alongside an improved metabolic rate and health. Thus, we show that phenotypic imputation at scale offers deeper biological insights into the genetics of human adiposity that could lead to therapeutic targets.
Phosphosite Scanning reveals a complex phosphorylation code underlying CDK-dependent activation of Hcm1
Authors: Conti Michelle M. , Li Rui , Narváez Ramos Michelle A. , Zhu Lihua Julie , Fazzio Thomas G. , Benanti Jennifer A.
Published: January 2023

Ordered cell cycle progression is coordinated by cyclin dependent kinases (CDKs). CDKs often phosphorylate substrates at multiple sites clustered within disordered regions. However, for most substrates, it is not known which phosphosites are functionally important. We developed a high-throughput approach, Phosphosite Scanning, that tests the importance of each phosphosite within a multisite phosphorylated domain. We show that Phosphosite Scanning identifies multiple combinations of phosphosites that can regulate protein function and reveals specific phosphorylations that are required for phosphorylation at additional sites within a domain. We applied this approach to the yeast transcription factor Hcm1, a conserved regulator of mitotic genes that is critical for accurate chromosome segregation. Phosphosite Scanning revealed a complex CDK-regulatory circuit that mediates Cks1-dependent phosphorylation of key activating sites in vivo. These results illuminate the mechanism of Hcm1 activation by CDK and establish Phosphosite Scanning as a powerful tool for decoding multisite phosphorylated domains.
Time-dependent unloading effects on muscle and bone and involvement of FNDC5/irisin axis
Authors: Sanesi Lorenzo , Storlino Giuseppina , Dicarlo Manuela , Oranger Angela , Zerlotin Roberta , Pignataro Patrizia , Suriano Clelia , Guida Gabriella , Grano Maria , Colaianni Graziana , Colucci Silvia Concetta
Published: January 2023

The identification of biomarkers and countermeasures to prevent the adverse effects on the musculoskeletal system caused by the absence of mechanical loading is the main goal of space biomedical research studies. In this study, we analyzed over 4 weeks of unloading, the modulation in the expression of key proteins in Vastus lateralis, Gastrocnemius and cortical bone in parallel with the modulation of irisin serum levels and its precursor FNDC5 in skeletal muscle of hind limb unloaded (HU) mice. Here we report that Atrogin-1 was up-regulated as early as 1- and 2-week of unloading, whereas Murf-1 at 2- and 3-weeks, along with a marked modulation in the expression of myosin heavy chain isoforms during unloading. Since HU mice showed reduced irisin serum levels at 4-weeks, as well as FNDC5 decrease at 3- and 4-weeks, we treated HU mice with recombinant irisin for 4 weeks, showing that unloading-dependent decline of myosin heavy chain isoforms, MyHCIIα and MyHCIIx, and the anti-apoptotic factor Bcl2, were prevented. In parallel, irisin treatment inhibited the increase of the senescence marker p53, and the pro-apoptotic factor Bax. Overall, these results suggest that the myokine irisin could be a possible therapy to counteract the musculoskeletal impairment caused by unloading.