bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of network public opinion on COVID-19 epidemic based on the WSR theory
Authors: Yang Kun , Zhu Junqi , Yang Li , Lin Yu , Huang Xin , Li YunPeng
Published: January 2023

Objective

To obtain the influencing factors of public opinion reactions and to construct a basic framework of the factors causing the occurrence of online public opinion in the epidemic area.

Methods

The hot news comments on microblogs during the epidemic in Shanghai were collected and analyzed with qualitative analysis, grounded theory, and the “Wuli-Shili-Renli” (WSR) methodology as an auxiliary method.

Results

(1) Three core categories of the Wuli system, the Shili system, and the Renli system, 15 main categories, and 86 categories that influence the development of network public opinion are obtained. (2) WSR Elements Framework Of Network Public Opinion (WSR-EFONPO) is established. (3) The WSR-EFONPO is explained.

Conclusion

The framework of factors for the occurrence of network public opinion is proposed, and the development process of network public opinion under COVID-19 is sorted out, which is of great theoretical value in guiding the public in the epidemic area to form reasonable behavior.
Zero-shot stance detection: Paradigms and challenges
Authors: Allaway Emily , McKeown Kathleen
Published: January 2023

A major challenge in stance detection is the large (potentially infinite) and diverse set of stance topics. Collecting data for such a set is unrealistic due to both the expense of annotation and the continuous creation of new real-world topics (e.g., a new politician runs for office). Furthermore, stancetaking occurs in a wide range of languages and genres (e.g., Twitter, news articles). While zero-shot stance detection in English, where evaluation is on topics not seen during training, has received increasing attention, we argue that this attention should be expanded to multilingual and multi-genre settings. We discuss two paradigms for English zero-shot stance detection evaluation, as well as recent work in this area. We then discuss recent work on multilingual and multi-genre stance detection, which has focused primarily on non-zero-shot settings. We argue that this work should be expanded to multilingual and multi-genre zero-shot stance detection and propose best practices to systematize and stimulate future work in this direction. While domain adaptation techniques are well-suited for work in these settings, we argue that increased care should be taken to improve model explainability and to conduct robust evaluations, considering not only empirical generalization ability but also the understanding of complex language and inferences.
Diagnosis and phylogenetic analysis of bovine leukemia virus in dairy cattle in northeastern Brazil
Authors: Pereira José Gomes , Silva Cândida de Assunção , Silva Lucas Diniz , Lima Cristian Alex Aquino , do Rosário Carla Janaina Rebouças Marques , Silva Ellainy Maria Conceição , Oliveira Maria do Socorro Costa , Ribeiro Larissa Sarmento dos Santos , Santos Hamilton Pereira , Abreu-Silva Ana Lucia , Melo Ferdinan Almeida
Published: January 2023

Enzootic bovine leukosis (EBL) is a chronic viral disease of wide distribution in cattle herds and may take several years for the first manifestation of clinical signs. Most animals do not present clinical signs. However, the economic losses are underestimated due to this disease. Thus, this work aimed to detect and characterize BLV in dairy cattle in the Maranhão state, northeastern Brazil. Blood samples were collected from 176 animals from 8 municipalities in the southeastern state of Maranhão. Bovine blood samples were subjected to DNA extraction and molecular diagnosis using nested PCR assays for BLV, targeting gp51 gene. Positive samples were then sequenced and then subjected to phylogenetic inferences. BLV DNA was detected in 16 cattle (16/176, 9.09%) in 4 municipalities. Phylogenetic analyzes showed that the sequence obtained clustered in a clade containing BLV sequences classified as genotype 6, with a high degree of support. Our data shows BLV occurrence in the Northeast of Brazil and the identification of genotype 6 in this region. These findings contribute to the molecular epidemiology of this agent in Brazil.
The multicomponent residue depletion of Gelsemium elegans in pig tissues, urine, and plasma
Authors: Wu Yong , Long Xue-Ming , Liu Gao-Feng , Bai Xia , Sun Zhi-Liang , Liu Zhao-Ying
Published: January 2023

Introduction

Gelsemium elegans (G. elegans) as a traditional medicinal plant used in livestock production. The use of G. elegans in veterinary clinics may pose safety risks to human health.

Objectives

The aim of this study was to investigate tissue residue depletion in pigs fed G. elegans powder.

Methods

A precise quantitation method and a simultaneous semi-quantitation method for multiple components independently of standards in pig tissues were developed for the first time. The two methods were validated in terms of specificity, LODs, LOQs, linearity, accuracy, precision, and matrix effects. They were then applied to a tissue residue depletion study after G. elegans powder at a dose of 2% per kg feed were fed to pigs.

Results

Compared with precise quantitation, the method validation results indicated that the semi-quantitation method was reliable and acceptable for multicomponent quantification independent of standards. Many G. elegans alkaloids are widely distributed in most tissues of pigs. Tissue residue depletion studies indicated that 14-hydroxygelsenicine, 11-hydroxygelsenicine, and gelsemoxonine could be used as potential residue markers, and pancreas, small intestine, and lung tissues could be considered as potential residue target tissues of G. elegans. In addition, both urine and plasma could be used to predict 14-hydroxygelsenicine and gelsemoxonine residues in the liver, pancreas, and small intestinal tissues of pigs.

Conclusion

The developed semi-quantification method can be applied to monitor the application and residue of G. elegans. The results provide scientific evidence for evaluating the safety of animal-derived food from G. elegans for consumers and will be helpful for its application and future development.
A Fine-Grained Bird Classification Method Based on Attention and Decoupled Knowledge Distillation
Authors: Wang Kang , Yang Feng , Chen Zhibo , Chen Yixin , Zhang Ying
Published: January 2023

Classifying birds accurately is essential for ecological monitoring. In recent years, bird image classification has become an emerging method for bird recognition. However, the bird image classification task needs to face the challenges of high intraclass variance and low inter-class variance among birds, as well as low model efficiency. In this paper, we propose a fine-grained bird classification method based on attention and decoupled knowledge distillation. First of all, we propose an attention-guided data augmentation method. Specifically, the method obtains images of the object’s key part regions through attention. It enables the model to learn and distinguish fine features. At the same time, based on the localization–recognition method, the bird category is predicted using the object image with finer features, which reduces the influence of background noise. In addition, we propose a model compression method of decoupled knowledge distillation. We distill the target and nontarget class knowledge separately to eliminate the influence of the target class prediction results on the transfer of the nontarget class knowledge. This approach achieves efficient model compression. With 67% fewer parameters and only 1.2 G of computation, the model proposed in this paper still has a 87.6% success rate, while improving the model inference speed.
Horse Housing on Prince Edward Island, Canada: Attitudes and Experiences Related to Keeping Horses Outdoors and in Groups
Authors: Ross Megan , Proudfoot Kathryn , Merkies Katrina , Elsohaby Ibrahim , Mills Molly , Macmillan Kathleen , Mckenna Shawn , Ritter Caroline
Published: January 2023

Limited research has assessed the “human dimension” of horse care. The aims of this study were to (1) understand horse owner attitudes toward horse welfare when kept outdoors versus indoors and in groups versus individually, (2) compare horse owner attitudes toward horse welfare with the ways in which they house their horses, and (3) explore horse owner reasons for and challenges with their horses’ housing. Seventy-six horse owners in Prince Edward Island, Canada completed a questionnaire. Non-parametric tests and quantitative content analysis were used for data analysis. Consistent with the way horses were kept, most (82–96%) owners agreed that horses’ physical health, mental well-being, and natural living were better when kept outdoors and in groups. Fewer (64–68%) participants agreed that the horses’ standard of care was better when kept outdoors or in groups. Results show associations between owners whose attitudes suggest indoor and/or individual housing is better for horse welfare and keeping their horses indoors part-time and/or individually. Two overarching themes were developed from owners’ responses regarding their reasons and challenges related to the ways in which horses were housed: horse-centered and owner-centered care. The results indicate that horse owners’ choices about their horses’ housing correspond to beliefs about improved horse welfare.
ACADL Promotes the Differentiation of Goat Intramuscular Adipocytes
Authors: Li An , Li Yanyan , Wang Youli , Wang Yong , Li Xin , Qubi Wuqie , Xiong Yan , Zhu Jiangjiang , Liu Wei , Lin Yaqiu
Published: January 2023

Intramuscular fat (IMF) deposits help improve meat quality such as marbling, juicy, flavor and tenderness. Long-chain acyl-CoA dehydrogenase (ACADL) is a key enzyme for catalyzing fatty acid oxidation, and studies have shown ACADL is involved in the deposition and differentiation of intramuscular adipocytes. However, the effect of ACADL on intramuscular adipocytes differentiation in goats needs further study. In this study, to explore the mechanism of ACADL on the development of goat intramuscular adipocytes, we constructed an over-expression plasmids and a SI-RNA of ACADL to explore the function of ACADL on the development of goat IMF. It was found that overexpression of ACADL promoted the differentiation of goat intramuscular adipocytes, and promoted the expression of fat cell differentiation marker genes lipoprotein lipase (LPL), peroxisome proliferator activated receptor gamma (PPARγ), APETALA-2-like transcription factor gene (AP2), CCAT enhancer binding protein (CEBPα), preadipocyte Factor 1 (Pref-1) and CCAT enhancer binding protein (CEBPβ), and the opposite trend occurred after interference. In addition, we screened of this related tumor necrosis factor (TNF) signaling pathway by RNA-Seq. So, we validate the signaling pathway with inhibitor of TNF signaling pathway. In summary, these results indicate that ACADL promotes intramuscular adipocytes differentiation through activation TNF signaling pathway. This study provides an important basis for the mechanism of IMF development.
Automatic Center Detection of Tropical Cyclone Using Image Processing Based on the Operational Radar Network
Authors: Mo Sun-Jin , Gu Ji-Young
Published: January 2023

This study presents the algorithm ACTION, defined as automatic center detection of tropical cyclone (TC) using image processing based on the operational radar network. Based on the high visibility of weather radar imagery, a TC’s motion vector is calculated from the continuous image change using optical flow, producing the TC’s rotation center. The algorithm performance was verified by analyzing the typhoons (TCs in the northwestern Pacific) that affected the Korean Peninsula from 2018–2021, demonstrating a high detection rate of 80.8% within an error distance of 40 km against the best track of the Korea Meteorological Administration (KMA). The detection rate was 100% for typhoons with temporally consistent morphological characteristics. ACTION automatically generates TC center information upon the TC’s initial entry inside the observation radius even in the absence of uniform radar data. As ACTION is capable of performing real-time calculations that are directly applied to rapidly generated weather radar images, it is currently being utilized by the KMA. The high temporal resolution TC center information calculated through ACTION is expected to improve the efficiency of TC forecasting.
A Rare Tropical Cyclone Necessitating the Issuance of Gale or Storm Wind Warning Signal in Hong Kong in Late Autumn in 2022—Severe Tropical Storm Nalgae
Authors: Chan Pak-Wai , Choy Chun-Wing , Mak Betty , He Junyi
Published: January 2023

The invaluable meteorological observations for a late autumn tropical cyclone that came very close to Hong Kong, namely, Nalgae in November 2022, are documented in this article. In particular, the dropsonde data for two consecutive days for this late season storm close to Hong Kong are presented. Meteorological data revealed that while Nalgae appeared to be rather weak from the meteorological satellite image under the cool sea surface water and the cool and dry northeast monsoon, it still maintained considerable intensity near the lower-boundary layer and managed to bring gale to storm force 10 min mean winds over many places in Hong Kong, necessitating the issuance of a gale or storm wind signal in November since 1972. The consideration in the issuance of the warning signal in Hong Kong and the difficulty in numerical weather prediction (NWP) model in forecasting Nalgae are also discussed in this article.
A Study on Monitoring and Supervision of Ship Nitrogen-Oxide Emissions and Fuel-Sulfur-Content Compliance
Authors: Wang Zheng , Ma Qianchi , Zhang Zhida , Li Zichao , Qin Cuihong , Chen Junfeng , Peng Chuansheng
Published: January 2023

Regulations for the control of air-pollutant emissions from ships within pollutant emission control areas (ECAs) have been issued for several years, but the lack of practical technologies and fundamental theory in the implementation process remains a challenge. In this study, we designed a model to calculate the nitrogen-oxide-emission intensity of ships and the sulfur content of ship fuels using theoretical deduction from the law of the conservation of mass. The reliability and availability of the derived results were empirically evaluated using measurement data for NOx, SO2, and CO2 in the exhaust gas of a demonstration ship in practice. By examining the model and the measured or registered fuel-oil-consumption rates of ships, a compliance-determination workflow for NOx-emission intensity and fuel-sulfur-content monitoring and supervision in on-voyage ships were proposed. The results showed that the ship fuel’s NOx-emission intensity and sulfur content can be evaluated by monitoring the exhaust-gas composition online and used to assist in maritime monitoring and the supervision of pollutant emissions from ships. It is recommended that uncertainties regarding sulfur content should be considered within 15% during monitoring and supervision. The established model and workflow can assist in maritime monitoring. Meanwhile, all related governments and industry-management departments are advised to actively lead the development of monitoring and supervision technology for ship-air-pollutant control in ECAs, as well as strengthening the quality management of ships’ static data.
Whole-Heart Tissue Engineering and Cardiac Patches: Challenges and Promises
Authors: Akbarzadeh Aram , Sobhani Soheila , Soltani Khaboushan Alireza , Kajbafzadeh Abdol-Mohammad
Published: January 2023

Despite all the advances in preventing, diagnosing, and treating cardiovascular disorders, they still account for a significant part of mortality and morbidity worldwide. The advent of tissue engineering and regenerative medicine has provided novel therapeutic approaches for the treatment of various diseases. Tissue engineering relies on three pillars: scaffolds, stem cells, and growth factors. Gene and cell therapy methods have been introduced as primary approaches to cardiac tissue engineering. Although the application of gene and cell therapy has resulted in improved regeneration of damaged cardiac tissue, further studies are needed to resolve their limitations, enhance their effectiveness, and translate them into the clinical setting. Scaffolds from synthetic, natural, or decellularized sources have provided desirable characteristics for the repair of cardiac tissue. Decellularized scaffolds are widely studied in heart regeneration, either as cell-free constructs or cell-seeded platforms. The application of human- or animal-derived decellularized heart patches has promoted the regeneration of heart tissue through in vivo and in vitro studies. Due to the complexity of cardiac tissue engineering, there is still a long way to go before cardiac patches or decellularized whole-heart scaffolds can be routinely used in clinical practice. This paper aims to review the decellularized whole-heart scaffolds and cardiac patches utilized in the regeneration of damaged cardiac tissue. Moreover, various decellularization methods related to these scaffolds will be discussed.
A Flexible Pressure Sensor Based on Silicon Nanomembrane
Authors: Cheng Lixia , Hao Xiaojian , Liu Guochang , Zhang Wendong , Cui Jiangong , Zhang Guojun , Yang Yuhua , Wang Renxin
Published: January 2023

With advances in new materials and technologies, there has been increasing research focused on flexible sensors. However, in most flexible pressure sensors made using new materials, it is challenging to achieve high detection sensitivity across a wide pressure range. Although traditional silicon-based sensors have good performance, they are not formable and, because of their rigidity and brittleness, they are not suitable for fitting with soft human skin, which limits their application in wearable devices to collect various signals. Silicon nanomembranes are ultra-thin, flexible materials with excellent piezoresistive properties, and they can be applied in various fields, such as in soft robots and flexible devices. In this study, we developed a flexible pressure sensor based on the use of silicon nanomembranes (with a thickness of only 340 nm) as piezoresistive units, which were transferred onto a flexible polydimethylsiloxane (PDMS) substrate. The flexible pressure sensor operated normally in the range of 0–200 kPa, and the sensitivity of the sensor reached 0.0185 kPa−1 in the low-pressure range of 0–5 kPa. In the high-pressure range of 5–200 kPa, the sensitivity of the sensor was maintained at 0.0023 kPa−1. The proposed sensor exhibited a fast response and excellent long-term stability and could recognize human movements, such as the bending of fingers and wrist joints, while maintaining a stable output. Thus, the developed flexible pressure sensor has promising applications in body monitoring and wearable devices.
How Functional Connectivity Measures Affect the Outcomes of Global Neuronal Network Characteristics in Patients with Schizophrenia Compared to Healthy Controls
Authors: Jonak Kamil , Marchewka Magdalena , Podkowiński Arkadiusz , Siejka Agata , Plechawska-Wójcik Małgorzata , Karpiński Robert , Krukow Paweł
Published: January 2023

Modern computational solutions used in the reconstruction of the global neuronal network arrangement seem to be particularly valuable for research on neuronal disconnection in schizophrenia. However, the vast number of algorithms used in these analyses may be an uncontrolled source of result inconsistency. Our study aimed to verify to what extent the characteristics of the global network organization in schizophrenia depend on the inclusion of a given type of functional connectivity measure. Resting-state EEG recordings from schizophrenia patients and healthy controls were collected. Based on these data, two identical procedures of graph-theory-based network arrangements were computed twice using two different functional connectivity measures (phase lag index, PLI, and phase locking value, PLV). Two series of between-group comparisons regarding global network parameters calculated on the basis of PLI or PLV gave contradictory results. In many cases, the values of a given network index based on PLI were higher in the patients, and the results based on PLV were lower in the patients than in the controls. Additionally, selected network measures were significantly different within the patient group when calculated from PLI or PLV. Our analysis shows that the selection of FC measures significantly affects the parameters of graph-theory-based neuronal network organization and might be an important source of disagreement in network studies on schizophrenia.
Actigraphic Wake after Sleep Onset and Symptom Severity Correspond with Rumination in Trauma-Exposed Individuals
Authors: Chang Fini , Berenz Erin C. , Ajilore Olusola , Langenecker Scott A. , Burgess Helen J. , Phan K. Luan , Klumpp Heide
Published: January 2023

Rumination and worry are forms of repetitive negative thinking (RNT) commonly associated with internalizing psychopathologies, although less is known about RNT in trauma-exposed individuals with internalizing psychopathologies. Separate lines of research show RNT also plays a role in problematic sleep, which is frequently experienced after trauma exposure. To address gaps in the literature, the current study examines the impact of sleep and symptoms on RNT in trauma-exposed participants. A transdiagnostic sample of 46 unmedicated treatment-seeking trauma-exposed participants completed standard measures of rumination and worry, as well as clinical measures that assessed posttraumatic stress, depression, and anxiety severity. Actigraphic sleep variables were sleep duration, wake after sleep onset (WASO), and sleep efficiency. Sleep and clinical measures were submitted to multiple regression analyses with rumination and worry as dependent variables. The regression results showed that rumination was significantly explained by WASO and posttraumatic stress symptom (PTSS) severity, and the omnibus test was significant. Depression, anxiety, and other estimates of sleep were not significant. No significant results emerged for worry. Preliminary findings suggest that PTSS and WASO, an index of fragmented sleep, may contribute to rumination, but not worry, in trauma-exposed individuals. Longitudinal studies are needed to determine potential causal relationships.
Acetazolamide for Bipolar Disorders: A Scoping Review
Authors: Strawbridge Rebecca , Yalin Nefize , Orfanos Stelios , Young Allan H.
Published: January 2023

Acetazolamide, a carbonic anhydrase inhibitor, is used to treat a variety of ailments. It has been highlighted for its potential to benefit people with bipolar disorders, for whom there are clear current unmet treatment needs. This scoping review sought to synthesise all available evidence related to the potential effects of acetazolamide on symptoms related to bipolar disorder, acceptability and tolerability, and intervention characteristics (e.g., dose and duration). Following publication of the review protocol, the Pubmed, Embase, and PsycInfo databases were searched (all dated to 31 August 2022). A systematic approach was undertaken to identify eligible articles and extract relevant data from these. Five studies were included, assessing a total of 50 patients treated with acetazolamide. Most patients were from two open-label trials, while the others were case reports. Approximately one third of patients were experiencing psychosis or mania before treatment initiation, and one third had refractory depression. Forty-four percent of patients were estimated to achieve a response (not seemingly affected by the baseline episode type, acetazolamide dose, or duration), while a further 22% appeared to experience minimal benefits from the intervention. Acetazolamide was generally reported to be tolerated well and acceptable for up to 2 years, although reporting for acceptability and tolerability was suboptimal. The reviewed evidence is extremely limited in size and methodology (e.g., no randomised studies, blinding, or standardised outcome assessment). We posit that the current findings are sufficiently encouraging to recommend substantive clinical trials, but we emphasise that at present, the evidence is exceedingly preliminary, and there remains evident uncertainty as to whether acetazolamide could be a viable treatment for bipolar disorders.
Are They the Same for All People? Nurses’ Knowledge about the Basic Human Needs of People with Disabilities
Authors: Ljubičić Marija , Burčul Ivona , Gusar Ivana , Šare Sonja
Published: January 2023

Nursing care involves a continuous interaction between nurses and people with disabilities. This has created a need for assessment tools that measure nurses’ knowledge about the basic human needs of people with disabilities. The aim of this cross-sectional study was to develop a Knowledge of Basic Human Needs Scale and investigate nurses’ levels of knowledge about the basic human needs of people with disabilities and their association with nurses’ education. Data were analyzed using principal component analysis to test the construct validity and to identify factors using principal varimax rotation. The reliability estimate was based on Cronbach’s alpha coefficient. Linear regression models were used to assess the association between knowledge about basic human needs and predictors. Factor analysis extracted eight factors, explaining 66.3% of the total variance. The sampling adequacy, criterion validity, and internal consistency were satisfactory. The nurses’ levels of education was associated with their knowledge about the basic human needs of people with disabilities. The questionnaire constitutes a valuable contribution to improving nurses’ knowledge and practice, as well as the quality of healthcare, and it provides a contribution to improving the quality of life for people with disabilities.
Inspection-Nerf: Rendering Multi-Type Local Images for Dam Surface Inspection Task Using Climbing Robot and Neural Radiance Field
Authors: Hong Kunlong , Wang Hongguang , Yuan Bingbing
Published: January 2023

For the surface defects inspection task, operators need to check the defect in local detail images by specifying the location, which only the global 3D model reconstruction can’t satisfy. We explore how to address multi-type (original image, semantic image, and depth image) local detail image synthesis and environment data storage by introducing the advanced neural radiance field (Nerf) method. We use a wall-climbing robot to collect surface RGB-D images, generate the 3D global model and its bounding box, and make the bounding box correspond to the Nerf implicit bound. After this, we proposed the Inspection-Nerf model to make Nerf more suitable for our near view and big surface scene. Our model use hash to encode 3D position and two separate branches to render semantic and color images. And combine the two branches’ sigma values as density to render depth images. Experiments show that our model can render high-quality multi-type images at testing viewpoints. The average peak signal-to-noise ratio (PSNR) equals 33.99, and the average depth error in a limited range (2.5 m) equals 0.027 m. Only labeled 2% images of 2568 collected images, our model can generate semantic masks for all images with 0.957 average recall. It can also compensate for the difficulty of manual labeling through multi-frame fusion. Our model size is 388 MB and can synthesize original and depth images of trajectory viewpoints within about 200 m2 dam surface range and extra defect semantic masks.
Obesity and Cancer: A Current Overview of Epidemiology, Pathogenesis, Outcomes, and Management
Authors: Pati Sukanya , Irfan Wadeed , Jameel Ahmad , Ahmed Shahid , Shahid Rabia K.
Published: January 2023

Background: Obesity or excess body fat is a major global health challenge that has not only been associated with diabetes mellitus and cardiovascular disease but is also a major risk factor for the development of and mortality related to a subgroup of cancer. This review focuses on epidemiology, the relationship between obesity and the risk associated with the development and recurrence of cancer and the management of obesity. Methods: A literature search using PubMed and Google Scholar was performed and the keywords ‘obesity’ and cancer’ were used. The search was limited to research papers published in English prior to September 2022 and focused on studies that investigated epidemiology, the pathogenesis of cancer, cancer incidence and the risk of recurrence, and the management of obesity. Results: About 4–8% of all cancers are attributed to obesity. Obesity is a risk factor for several major cancers, including post-menopausal breast, colorectal, endometrial, kidney, esophageal, pancreatic, liver, and gallbladder cancer. Excess body fat results in an approximately 17% increased risk of cancer-specific mortality. The relationship between obesity and the risk associated with the development of cancer and its recurrence is not fully understood and involves altered fatty acid metabolism, extracellular matrix remodeling, the secretion of adipokines and anabolic and sex hormones, immune dysregulation, and chronic inflammation. Obesity may also increase treatment-related adverse effects and influence treatment decisions regarding specific types of cancer therapy. Structured exercise in combination with dietary support and behavior therapy are effective interventions. Treatment with glucagon-like peptide-1 analogues and bariatric surgery result in more rapid weight loss and can be considered in selected cancer survivors. Conclusions: Obesity increases cancer risk and mortality. Weight-reducing strategies in obesity-associated cancers are important interventions as a key component of cancer care. Future studies are warranted to further elucidate the complex relationship between obesity and cancer with the identification of targets for effective interventions.
Pathology of Combined Hepatocellular Carcinoma-Cholangiocarcinoma: An Update
Authors: Roßner Florian , Sinn Bruno Valentin , Horst David
Published: January 2023

Combined hepatocellular carcinoma-cholangiocarcinoma (cHCC-CCA) is a rare primary liver cancer that is composed of both hepatocellular and cholangiocellular differentiated cells. It is slightly more common in men and among Asian and Pacific islanders. Overall, risk factors are similar to classic risk factors of hepatocellular carcinoma (HCC). The classification has significantly evolved over time. The last WHO classification (2019) mainly emphasized diagnosis on morphological basis with routine stainings, discarded previously recognized classifications with carcinomas with stem cell features, introduced intermediate cell carcinoma as a specific subtype and considered cholangiolocarcinoma as a subtype of cholangiocellular carcinoma. Immunohistochemical markers may be applied for further specification but have limited value for diagnosis. Recent discoveries in molecular pathway regulation may pioneer new therapeutic approaches for this poor prognostic and challenging diagnosis.
Real-Life Study of the Benefit of Concomitant Radiotherapy with Cemiplimab in Advanced Cutaneous Squamous Cell Carcinoma (cSCC): A Retrospective Cohort Study
Authors: Bailly-Caillé Barbara , Kottler Diane , Morello Rémy , Lecornu Marie , Kao William , Meyer Emmanuel , Dompmartin Anne , L’Orphelin Jean-Matthieu
Published: January 2023

Background: Cemiplimab is a monoclonal antibody targeting the PD-1, and phase II trials have shown its efficacy in the treatment of advanced cutaneous squamous cell carcinoma in patients who are not candidates for curative surgery or radiation therapy as a first- or later-line treatment. A synergistic antitumoral response has been demonstrated with concurrent radiotherapy and PD1-immunotherapy. However, no real-life study has demonstrated this effect in advanced cutaneous squamous cell carcinoma. Methods: We conducted a real-life retrospective cohort study to investigate the benefit of concomitant therapy in 33 patients treated with cemiplimab at the University Hospital of Caen, alone (C group) or concomitant to radiotherapy (C/RT group). Our primary objectives were to evaluate the best overall response and objective response rate. Our secondary objectives were the disease control rate, median time to response, progression-free survival, overall survival, clinical benefit of radiotherapy, and safety data. After stopping cemiplimab administration, we performed a follow-up of our patients and performed a descriptive study. Results: We reported an objective response rate of 45.5%, including 47.6% in the cemiplimab group versus 41.6% in the concomitant group. The addition of radiotherapy to cemiplimab enables an earlier clinico-radiological response, with a median duration of 5.5 months in the cemiplimab group versus 3 months in the concomitant therapy group. The response to treatment was prolonged despite discontinuation of cemiplimab, with 91.6% (n = 11/12) and 83.3% (n = 10/12) patients in complete or partial remission at 6 months and 1 year after cessation of cemiplimab and no switch to another oncological treatment, respectively. Radiation therapy also provided a therapeutic effect in 83.3% of the patients in the concomitant group, without increasing the occurrence of adverse events. Conclusions: Our study confirms the efficacy of cemiplimab and radiotherapy in the management of advanced cutaneous squamous cell carcinoma. Concomitant therapy permitted to obtain an earlier radiological response, a beneficial local therapeutic effect of radiotherapy, without any safety alert.
Shaping Up the Tumor Microenvironment: Extracellular Vesicles as Important Intermediaries in Building a Tumor-Supportive Cellular Network
Authors: Sharma Amod , Singh Ajay Pratap , Singh Seema
Published: January 2023

A tumor is not just comprised of cancer cells but also a heterogeneous group of infiltrating and resident host cells, as well as their secreted factors that form the extracellular matrix [...]
Disruption of the Molecular Regulation of Mitochondrial Metabolism in Airway and Lung Epithelial Cells by Cigarette Smoke: Are Aldehydes the Culprit?
Authors: Tulen Christy B. M. , Opperhuizen Antoon , van Schooten Frederik-Jan , Remels Alexander H. V.
Published: January 2023

Chronic obstructive pulmonary disease (COPD) is a devastating lung disease for which cigarette smoking is the main risk factor. Acetaldehyde, acrolein, and formaldehyde are short-chain aldehydes known to be formed during pyrolysis and combustion of tobacco and have been linked to respiratory toxicity. Mitochondrial dysfunction is suggested to be mechanistically and causally involved in the pathogenesis of smoking-associated lung diseases such as COPD. Cigarette smoke (CS) has been shown to impair the molecular regulation of mitochondrial metabolism and content in epithelial cells of the airways and lungs. Although it is unknown which specific chemicals present in CS are responsible for this, it has been suggested that aldehydes may be involved. Therefore, it has been proposed by the World Health Organization to regulate aldehydes in commercially-available cigarettes. In this review, we comprehensively describe and discuss the impact of acetaldehyde, acrolein, and formaldehyde on mitochondrial function and content and the molecular pathways controlling this (biogenesis versus mitophagy) in epithelial cells of the airways and lungs. In addition, potential therapeutic applications targeting (aldehyde-induced) mitochondrial dysfunction, as well as regulatory implications, and the necessary required future studies to provide scientific support for this regulation, have been covered in this review.
An Inhibitory Role for Human CD96 Endodomain in T Cell Anti-Tumor Responses
Authors: Wang Chelsia Qiuxia , Choy Fong Chan , Sanny Arleen , Murakami Takashi , Tan Andy Hee-Meng , Lam Kong-Peng
Published: January 2023

Immune checkpoint blockade (ICB) therapy involves the inhibition of immune checkpoint regulators which reverses their limitation of T cell anti-tumor responses and results in long-lasting tumor regression. However, poor clinical response or tumor relapse was observed in some patients receiving such therapy administered via antibodies blocking the cytotoxic T lymphocyte-associated protein 4 (CTLA-4) or the programmed cell death 1 (PD-1) pathway alone or in combination, suggesting the involvement of additional immune checkpoints. CD96, a possible immune checkpoint, was previously shown to suppress natural killer (NK) cell anti-tumor activity but its role in human T cells remains controversial. Here, we demonstrate that CRISPR/Cas9-based deletion of CD96 in human T cells enhanced their killing of leukemia cells in vitro. T cells engineered with a chimeric antigen receptor (CAR) comprising human epidermal growth factor receptor 2 (EGFR2/HER2)-binding extracellular region and intracellular regions of CD96 and CD3ζ (4D5-96z CAR-T cells) were less effective in suppressing the growth of HER2-expressing tumor cells in vitro and in vivo compared with counterparts bearing CAR that lacked CD96 endodomain (4D5-z CAR-T cells). Together, our findings implicate a role for CD96 endodomain in attenuating T cell cytotoxicity and support combination tumor immunotherapy targeting multiple rather than single immune checkpoints.
In Situ and Ex Situ Raman Studies of Cysteine’s Behavior on a Titanium Surface in Buffer Solution
Authors: Święch Dominika , Piergies Natalia , Palumbo Gaetano , Paluszkiewicz Czesława
Published: January 2023

In this paper, surface-enhanced Raman spectroscopy (SERS) was used to investigate the adsorption process of cysteine (Cys). Studies were carried out in the presence of phosphate-buffered saline solution (PBS), at pH 7.4, and acidified to pH 5, 3, and 1, on the surface of Ti for implant application. In situ SERS spectra obtained for the Cys/Ti solution system, after 24 h of immersion time, indicated that the buffer solution strongly influences the adsorption behavior of Cys on the Ti surface. This results in a decrease in Cys adsorption on the Ti surface, in the range of pH 7.4 to 3. The strong interaction between a sulfur atom of Cys and a Ti surface was observed only at pH = 1, under strongly acidic conditions. In contrast, ex situ SERS spectra recorded for the same samples but in a dried Cys/Ti system show a completely different behavior of Cys on the Ti surface. Formation of a disulfide (S-S) bond has occurred as a result of the dimerization or aggregation of Cys molecules on the Ti surface. Detailed analysis of the adsorption behavior of Cys on the Ti surface can be very important in the preparation of bioactive materials (i.e., coated by organic layers).
Advanced Corrosion Protection through Coatings and Surface Rebuilding
Authors: Saleh Bassiouny , Fathi Reham , Shi Hongwei , Wei Hongyu
Published: January 2023

Corrosion is a costly and dangerous phenomenon that affects many industries, leading to significant economic losses and, in some cases, catastrophic failures [...]
Plant Growth-Promoting Bacteria (PGPB) with Biofilm-Forming Ability: A Multifaceted Agent for Sustainable Agriculture
Authors: Ajijah Nur , Fiodor Angelika , Pandey Alok Kumar , Rana Anuj , Pranaw Kumar
Published: January 2023

Plant growth-promoting bacteria (PGPB) enhance plant growth, as well as protect plants from several biotic and abiotic stresses through a variety of mechanisms. Therefore, the exploitation of PGPB in agriculture is feasible as it offers sustainable and eco-friendly approaches to maintaining soil health while increasing crop productivity. The vital key of PGPB application in agriculture is its effectiveness in colonizing plant roots and the phyllosphere, and in developing a protective umbrella through the formation of microcolonies and biofilms. Biofilms offer several benefits to PGPB, such as enhancing resistance to adverse environmental conditions, protecting against pathogens, improving the acquisition of nutrients released in the plant environment, and facilitating beneficial bacteria–plant interactions. Therefore, bacterial biofilms can successfully compete with other microorganisms found on plant surfaces. In addition, plant-associated PGPB biofilms are capable of protecting colonization sites, cycling nutrients, enhancing pathogen defenses, and increasing tolerance to abiotic stresses, thereby increasing agricultural productivity and crop yields. This review highlights the role of biofilms in bacterial colonization of plant surfaces and the strategies used by biofilm-forming PGPB. Moreover, the factors influencing PGPB biofilm formation at plant root and shoot interfaces are critically discussed. This will pave the role of PGPB biofilms in developing bacterial formulations and addressing the challenges related to their efficacy and competence in agriculture for sustainability.
Immunotherapy Assessment: A New Paradigm for Radiologists
Authors: Granata Vincenza , Fusco Roberta , Setola Sergio Venanzio , Simonetti Igino , Picone Carmine , Simeone Ester , Festino Lucia , Vanella Vito , Vitale Maria Grazia , Montanino Agnese , Morabito Alessandro , Izzo Francesco , Ascierto Paolo Antonio , Petrillo Antonella
Published: January 2023

Immunotherapy denotes an exemplar change in an oncological setting. Despite the effective application of these treatments across a broad range of tumors, only a minority of patients have beneficial effects. The efficacy of immunotherapy is affected by several factors, including human immunity, which is strongly correlated to genetic features, such as intra-tumor heterogeneity. Classic imaging assessment, based on computed tomography (CT) or magnetic resonance imaging (MRI), which is useful for conventional treatments, has a limited role in immunotherapy. The reason is due to different patterns of response and/or progression during this kind of treatment which differs from those seen during other treatments, such as the possibility to assess the wide spectrum of immunotherapy-correlated toxic effects (ir-AEs) as soon as possible. In addition, considering the unusual response patterns, the limits of conventional response criteria and the necessity of using related immune-response criteria are clear. Radiomics analysis is a recent field of great interest in a radiological setting and recently it has grown the idea that we could identify patients who will be fit for this treatment or who will develop ir-AEs.
Spatial Prioritization of Ecosystem Services for Land Conservation: The Case Study of Central Italy
Authors: Sebastiani Alessandro , Fares Silvano
Published: January 2023

Ecosystem services delivered by natural ecosystems are increasingly important for climate change adaptation and mitigation and play a huge role in biodiversity conservation. For this reason, the EU has the ambitious goal of protecting at least 30% of land by 2030. Member states are called to improve and expand the network of protected areas within the next few years; to do so, scientific studies aimed at identifying areas with high ecological value, as well as at defining best management practices, are highly needed. In this study, we used the InVEST suite of models to spatially assess three regulating ecosystem services, that is, carbon storage, seasonal water yield, and urban flood risk mitigation in three administrative regions of central Italy. Using overlay analysis, we found areas with the highest delivery in each of the considered ESs; based on these findings, we eventually proposed four new protected areas, which combine for 888 km2, that is, 2.73% of the study area. Interestingly, each of the newly proposed protected areas has somehow been discussed and hypothesized by stakeholders, but only one is presumably going to be part of the national network of protected areas within the next years. Hopefully, by prioritizing areas according to the production of ecosystem services, this study can be intended as a step towards the systematic inclusion of ecosystem services studies for enhancing the network of areas under national protection schemes and achieving the goal of protecting at least 30% of land in Europe by 2030.
Predicting Mangrove Distributions in the Beibu Gulf, Guangxi, China, Using the MaxEnt Model: Determining Tree Species Selection
Authors: Li Lifeng , Liu Wenai , Ai Jingwen , Cai Shuangjiao , Dong Jianwen
Published: January 2023

Mangrove restoration is challenging within protected coastal habitats. Predicting the dominant species distributions in mangrove communities is essential for appropriate species selection and spatial planning for restoration. We explored the spatial distributions of six mangrove species, including their related environmental factors, thereby identifying potentially suitable habitats for mangrove protection and restoration. Based on six dominant mangrove species present in the Beibu Gulf, Guangxi, China, we used a linear correlation analysis to screen environmental factors. In addition, we used the maximum entropy model to analyze the spatial distributions of potential mangrove afforestation areas. Based on the spatial superposition analysis, we identified mangrove conservation and restoration hot spots. The findings indicate that topographic and bioclimatic factors affect the distribution of suitable mangrove habitats in the Beibu Gulf, followed by land use type, salinity, and substrate type. We identified 13,816 hm2 of prime mangrove habitat in the Beibu Gulf that is primarily distributed in protected areas. The protection rate for existing mangroves was 42.62%. According to the predicted spatial distributions of the mangrove plants, the findings suggest that mangrove restoration should be based on suitable species and site selection.
Identification of Alnus incana (L.) Moenx. × Alnus glutinosa (L.) Gaertn. Hybrids Using Metabolic Compounds as Chemotaxonomic Markers
Authors: Jurkšienė Girmantė , Sirgedaitė-Šėžienė Vaida , Juškauskaitė Aušra , Baliuckas Virgilijus
Published: January 2023

Alnus glutinosa (L.) Gaertn. and Alnus incana (L.) Moenx. grow naturally in Lithuania, and their ranges overlap. They are considered ecologically and economically important species of forest trees. The objective of our study was to determine plant bioactive compounds, such as total phenolic (TPC) and flavonoid compounds (TFC), in the wood of alders and their hybrids in order to specify the opportunity to use secondary metabolites (SM) for the identification of alder hybrids. The samples from juvenile and mature alder woods (n = 270) were collected at three different sites in the natural forests of Lithuania. The TPC and TFC content was determined using spectrophotometric methods and was expressed in mg/g of fresh mass. Obtained results showed that the TPC of A. incana was statistically higher compared to A. glutinosa; however, in hybrid alder wood it was intermediate. The TFC was statistically higher in hybrid alder wood and lowest in A. glutinosa. The TFC was higher in mature trees; however, the TPC showed the opposite tendency. In our case, the TPC was higher in continental sites, while TFC was higher in mature alders at costal sites. Obtained data allow us to assume that TPC and TFC in alder wood can be used as taxonomic markers. This study expanded the knowledge of alder physiology and contributed to the identification of alder hybrids. The correct identification of tree species is very important for the conservation of natural resources and for the sustainable use of higher value-added products.