bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Angiotensin II, Retinoic Acid, EGCG, and Vitamin C on the Cardiomyogenic Differentiation Induction of Human Amniotic Fluid-Derived Mesenchymal Stem Cells
Authors: Gasiūnienė Monika , Valatkaitė Elvina , Navakauskaitė Aistė , Navakauskienė Rūta
Published: November 2020

Human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) may be potentially applied in cell therapy or regenerative medicine as a new alternative source of stem cells. They could be particularly valuable in restoring cardiac tissue after myocardial infarction or other cardiovascular diseases. We investigated the potential of biologically active compounds, namely, angiotensin II, retinoic acid (RA), epigallocatechin-3-gallate (EGCG), vitamin C alone, and the combinations of RA, EGCG, and vitamin C with angiotensin II to induce cardiomyogenic differentiation of AF-MSCs. We observed that the upregulated expression of cardiac gene markers (NKX2-5, MYH6, TNNT2, and DES) and cardiac ion channel genes (sodium, calcium, the potassium) as well as the increased levels of Connexin 43 and Nkx2.5 proteins. Extracellular flux analysis, applied for the first time on AF-MSCs induced with biologically active compounds, revealed the switch in AF-MSCs energetic phenotype and enhanced utilization of oxidative phosphorylation for energy production. Moreover, we demonstrated changes in epigenetic marks associated with transcriptionally active (H3K4me3, H3K9ac, and H4hyperAc) or repressed (H3K27me3) chromatin. All in all, we demonstrated that explored biomolecules were able to induce alterations in AF-MSCs at the phenotypic, genetic, protein, metabolic, and epigenetic levels, leading to the formation of cardiomyocyte progenitors that may become functional heart cells in vitro or in vivo.
Newborn Screening for Mucopolysaccharidosis I: Moving Forward Learning from Experience
Authors: Clarke Lorne A. , Dickson Patricia , Ellinwood N. Matthew , Klein Terri L.
Published: November 2020

There have been significant advances allowing for the integration of mucopolysaccharidosis I into newborn screening programs. Initial experiences using a single-tier approach for this disorder have highlighted shortcomings that require immediate remediation. The recent evaluation of a second-tier biomarker integrated into the MPS I newborn screening protocol has been demonstrated to greatly improve the precision and predictive value of newborn screening for this disorder. This commentary urges newborn screening programs to learn from these experiences and improve newborn screening for mucopolysaccharidosis I and future mucopolysaccharidoses newborn screening programs by implementation of a second-tier biomarker analyte.
Chemosensory-Related Gene Family Members of the Horn Fly, Haematobia irritans irritans (Diptera: Muscidae), Identified by Transcriptome Analysis
Authors: Olafson Pia Untalan , Saski Christopher A.
Published: November 2020

Horn flies are one of the most significant economic pests of cattle in the United States and worldwide. Chemical control methods have been routinely utilized to reduce populations of this pest, but the steady development of insecticide resistance has prompted evaluation of alternative control strategies. Behavior modifying compounds from natural products have shown some success in impacting horn fly populations, and a more thorough understanding of the horn fly chemosensory system would enable improvements in the development of species-specific compounds. Using an RNA-seq approach, we assembled a transcriptome representing genes expressed in adult female and male horn fly head appendages (antennae, maxillary palps, and proboscides) and adult fly bodies from which heads were removed. Differential gene expression analysis identified chemosensory gene family members that were enriched in head appendage tissues compared with headless bodies. Candidate members included 43 odorant binding proteins (OBP) and 5 chemosensory binding proteins (CSP), as well as 44 odorant receptors (OR), 27 gustatory receptors (GR), and 34 ionotropic receptors (IR). Sex-biased expression of these genes was not observed. These findings provide a resource to enable future studies targeting horn fly chemosensation as part of an integrated strategy to control this blood-feeding pest.
Increased Institutional Surgical Experience in Robot-Assisted Radical Hysterectomy for Early Stage Cervical Cancer Reduces Recurrence Rate: Results from a Nationwide Study
Authors: Ekdahl Linnea , Wallin Emelie , Alfonzo Emilia , Reynisson Petur , Lönnerfors Celine , Dahm-Kähler Pernilla , Falconer Henrik , Persson Jan
Published: November 2020

The aim of this study was to evaluate the impact of institutional surgical experience on recurrence following robotic radical hysterectomy (RRH) for early stage cervical cancer. All women in Sweden who underwent an RRH for stage IA2-IB1 cervical cancer at tertiary referral centers from its implementation in December 2005 until June 2017 were identified using a Swedish nationwide register and local hospital registers. Registry data were controlled by a chart review of all women. Recurrence rates and patterns of recurrence were compared between early and late (≤50 vs. >50 procedures) institutional series. Six hundred and thirty-five women were included. Regression analysis identified a lower risk of recurrence with increased experience but without a clear cut off level. Among the 489 women who did not receive adjuvant radio chemotherapy (RC-T), the rate of recurrence was 3.6% in the experienced cohort (>50 procedures) compared to 9.3% in the introductory cohort (p < 0.05). This was also seen in tumors < 2 cm regardless of RC-T (p < 0.05), whereas no difference in recurrence was seen when analyzing all women receiving RC-T. In conclusion, the rate of recurrence following RRH for early stage cervical cancer decreased with increased institutional surgical experience, in tumors < 2 cm and in women who did not receive adjuvant RC-T.
Does Warm-up Type Matter? A Comparison between Traditional and Functional Inertial Warm-up in Young Soccer Players
Authors: Fiorilli Giovanni , Quinzi Federico , Buonsenso Andrea , Di Martino Giulia , Centorbi Marco , Giombini Arrigo , Calcagno Giuseppe , di Cagno Alessandra
Published: November 2020

Functional inertial training, a popular high-intensity training mode, provides high neuromuscular activation, developing proprioception, postural control, power, and sprint time. Aim of the study was to assess the acute effects of two types of warm-up (WU), inertial warm-up (IWU) vs. traditional warm-up (TWU), on explosive and reactive strength, sprint, and Change of Directions (COD) in young soccer players. In a randomized cross-over design study, twelve soccer players (aged 13.3 ± 0.7) performed 16 min of IWU and 16 min of TWU. IWU and TWU were spaced two weeks apart. Pre and post intervention tests, aimed at assessing explosive and reactive strength, sprint, and COD ability included: Squat Jump test (SJ), Countermovement Jump test (CMJ), Drop Jump test (DJ), Seven Repetition Hopping test (7R-HOP), 40 m-sprint test (40 m), and Illinois Agility Test (IAT). RM-ANOVA, used to compare differences between IWU and TWU effects (the level of significance set at ρ ≤ 0.05), showed enhanced performance after the IWU compared to the TWU. In addition, the effects of the IWU on performance lasted longer after the IWU than after the TWU. For IAT, the enhanced effects of IWU on performance lasted up to ten minutes after the administration of the IWU. Our results suggest that IWU affects functional changes displaying earlier adaptation in explosive and reactive strength with longer lasting effects compared to TWU and it could be recommended in young soccer athletes as a WU procedure.
Review of the Design and Technology Challenges of Zero-Emission, Battery-Driven Fast Marine Vehicles
Authors: Papanikolaou Apostolos D.
Published: November 2020

The paper deals with a critical review of unique problems and challenges related to the design and technology of zero-emission, battery driven, fast marine vehicles. The uniqueness of the ensuing ship design problem is the request to fit maximum battery capacity and to ensure minimum required power in order to achieve the set operational requirements for high service speed and sufficient range. The high-speed requirement is inherently connected with the request for minimum structural and lightship weight, while the design needs also to comply with set regulatory safety constraints. The underlying research is in the frame of the EU funded project TrAM (Transport: Advanced and Modular) and leads to the development and construction of a physical demonstrator for operation in the Stavanger area in Norway. The paper discusses the incurring critical issues, discusses the feasibility of the concept and concludes on the way ahead.
Biomonitoring of the Application of Monoculture and Integrated Multi-Trophic Aquaculture (IMTA) Using Macrobenthic Structures at Tembelas Island, Kepulauan Riau Province, Indonesia
Authors: Putro Sapto P. , Sharani Jeanny , Widowati  , Adhy Satriyo , Suryono
Published: November 2020

Sustainable aquaculture needs to be considered when it comes to the utilization of water resources. The aim of this study was to apply biomonitoring using macrobenthic structures on both spatial and temporal applications of monoculture and integrated multi-trophic aquaculture (IMTA) at Tembelas Island, Kepulauan Riau Province, Indonesia. Samples of sediment were taken from three fish farm locations, namely from an IMTA site, a monoculture site, and a reference site. Macrobenthic organisms obtained through rinsing, sieving, and sorting were then identified under a stereo-microscope. Diversity of the macrobenthic assemblages was analyzed with a Shannon-Wiener index (H’). Equitability was expressed through Pielou’s evenness index. Finally, Bray-Curtis’ non-metric multi-dimensional scaling (NMDS) was used for similarities derived from log (X+1) transformed macrobenthic abundance to provide a visual representation of differences in their structure between sites over time. Results showed polychaetes exhibited differences in both variation and abundance of genera between the farm and reference site. The assemblage of macrobenthos at the IMTA site consisted of 9 genera of gastropods, 3 genera of bivalves, 5 genera of polychaetes, and 2 genera of crustaceans. At the monoculture site, 12 genera of gastropods, 4 genera of bivalves, 8 genera of polychaetes, 1 genera of crustaceans, and 1 genera of ophiuroid were observed. A relatively high abundance was observed at the reference site, with 27 genera of gastropods, 11 genera of bivalves, 3 genera of polychaetes, and 1 genera of crustacean. The favorable water conditions and possible absence of waste input from aquaculture resulting in a more suitable habitat for macrobenthic life may explain this relative abundance. Some of them were recognised as opportunistic taxa, i.e., Capitella sp., Heteromastus sp., and Lumbrinereis sp. Based on the diversity and evenness indices and the MNDS ordination, it can be concluded that the application of IMTA systems results in a suppressed or reduced potential impact on environmental disturbance due to aquacultural activities.
Urbanization Chaos of Suburban Small Cities in Poland: ‘Tetris Development’
Authors: Majewska Anna , Denis Małgorzata , Krupowicz Wioleta
Published: November 2020

This paper investigates the phenomenon of spatial chaos in Poland resulting from urban sprawl. The phenomenon is particularly visible in the case of suburban small cities which, in contrast to cities in the EU-15 countries with similar populations, are expanding excessively, causing a growth of urbanized areas exceeding several times the growth of their population. Suburbs of these cities increasingly resemble a badly played Tetris game. The selected study area consists of several cities in the Warsaw suburban zone where an increased dynamic of these processes can be observed. The paper presents detailed studies concerning the selected representative small cities. The morphology of urban tissue was studied as a marker of spatial order including: development intensity, street grid, plots parameters, presence of technical infrastructure, and distance from the functional city center. The analyses were performed based on cartographic archives, the data of the Central Statistical Office of Poland, topographic database and Kernel Density Estimation. ArcGIS ESRI and AutoCad software was used to present the study results. The conducted studies intend to diagnose the changes in the spatial layout in the context of the objectives of spatial order and sustainable development, and to define the indicators which should be taken into account in spatial planning documents drawn up for the studied areas.
Retrospective Analysis of Permafrost Landscape Evolution in Yakutia during the Holocene Warm Intervals
Authors: Fedorov Alexander N. , Novopriezzhaya Varvara A. , Fedorov Nikolay A. , Konstantinov Pavel Y. , Samsonova Vera V.
Published: November 2020

The observed global warming has significant impacts on permafrost. Permafrost changes modify landscapes and cause damage to infrastructure. The main purpose of this study was to estimate permafrost temperatures and active-layer thicknesses during the Holocene intervals with significantly warmer-than-present climates—the Atlantic (5500 years BP), Subboreal (3500 years BP) and Subatlantic (1000 years BP) optimums. Estimates were obtained using the ready-to-use models derived by G.M. Feldman, as well as mathematical modeling taking account of the paleogeography of the Holocene warm intervals. The data obtained were analyzed to reveal the regional patterns of warming impacts on different permafrost landscapes. The study results will be useful in predicting future permafrost changes in response to climate warming.
Cost-Benefit Analysis of Landscape Restoration: A Stocktake
Authors: Wainaina Priscilla , Minang Peter A. , Gituku Eunice , Duguma Lalisa
Published: November 2020

With the increase in demand for landscape restoration and the limited resources available, there is need for economic analysis of landscape restoration to help prioritize investment of the resources. Cost-benefit analysis (CBA) is a commonly applied tool in the economic analysis of landscape restoration, yet its application seems limited and varied. We undertake a review of CBA applications to understand the breadth, depth, and gaps. Of the 2056 studies identified in literature search, only 31 met our predefined criteria. Three studies offered a global perspective, while more than half were conducted in Africa. Only six countries benefit from at least 2 CBA studies, including Brazil, Ethiopia, Kenya, Vietnam, South Africa, and Tanzania. About 60% focus on agroforestry, afforestation, reforestation, and assisted natural regeneration practices. Only 16% covered all cost categories, with opportunity costs being the least covered. Eighty-four percent apply direct use values, while only 16% captured the non-use values. Similarly, lack of reliable data due to predictions and assumptions involved in data generation influenced CBA results. The limited number of eligible studies and the weaknesses identified hereinabove suggest strong need for improvements in both the quantity and quality of CBA to better inform planning, policies, and investments in landscape restoration.
Infrared Laser Application to Wood Cutting
Authors: Aniszewska Monika , Maciak Adam , Zychowicz Witold , Zowczak Włodzimierz , Mühlke Thorsten , Christoph Bjoern , Lamrini Samir , Sujecki Sławomir
Published: November 2020

While lasers are widely used across various industries, including woodworking, few studies to date have addressed the issue of cutting fresh wood. In the present investigation, wood stemming from fresh tree branches was cut at different laser powers and beam travel speeds. A fiber laser and a CO2 laser were used for the research. The cellular structures of the cut surfaces were examined, with some of them found to be covered with a layer of compacted, charred cells. This may be a favorable phenomenon, preventing the invasion of pathogens via the wounds caused by laser beam branch cutting in nurseries, plantations, and orchards.
Research on the Properties and Low Cycle Fatigue of Sc-Modified AA2519-T62 FSW Joint
Authors: Kosturek Robert , Śnieżek Lucjan , Torzewski Janusz , Ślęzak Tomasz , Wachowski Marcin , Szachogłuchowicz Ireneusz
Published: November 2020

The aim of this research was to examine the mechanical and fatigue properties of friction stir welded Sc-modified 5 mm thick AA2519-T62 extrusion. The joint was obtained using the following parameters: 800 rpm tool rotation speed, 100 mm/min tool traverse speed, 17 kN axial, and MX Triflute as a tool. The investigation has involved microstructure observations, microhardness distribution analysis, tensile test with digital image correlation technique, observations of the fracture surface, measurements of residual stresses, low cycle fatigue testing, and fractography. It was stated that the obtained weld is defect-free and has joint efficiency of 83%. The failure in the tensile test occurred at the boundary of the thermo-mechanically affected zone and stir zone on the advancing side of the weld. The residual stress measurements have revealed that the highest values of longitudinal stress are localized at the distance of 10 mm from the joint line with their values of 124 MPa (the retreating side) and 159 MPa (the advancing side). The results of low cycle fatigue testing have allowed establishing of the values of the cyclic strength coefficient (k′ = 504.37 MPa) and cyclic strain hardening exponent (n′ = 0.0068) as well as the factors of the Manson–Coffin–Basquin equation: the fatigue strength coefficient σ′f = 462.4 MPa, the fatigue strength exponent b = −0.066, the fatigue ductility coefficient ε′f = 0.4212, and the fatigue ductility exponent c = −0.911.
Analysis of Overturning and Vibration during Field Operation of a Tractor-Mounted 4-Row Radish Collector toward Ensuring User Safety
Authors: Chowdhury Milon , Islam Md Nafiul , Iqbal Md Zafar , Islam Sumaiya , Lee Dae-Hyun , Kim Dae-Geon , Jun Hyeon-Jong , Chung Sun-Ok
Published: November 2020

The overturning stability and vibration of upland crop machinery under development are important issues for analysis because farms for upland crops are usually uneven, which may cause work-related fatalities, and vibration affects user comfort and reduces the durability of components. In this study, the overturning stability and vibration of a tractor-mounted radish collector were investigated to ensure safety during radish collection. To analyze lateral stability, the center of gravity (CG) of the tractor-mounted radish collector system was calculated mathematically. Then, a simulation was performed to determine the lateral overturning angles at different folding positions of the radish conveyor belt and load conditions, and the results were validated through tests. Vibration sensors were used to measure the vibration levels and the power spectrum density (PSD) was obtained to check the cyclic apparatuses of the major frequencies. The load conditions, different conveyor speeds, and locations were considered as factors affecting the vibration levels. Considering the physical parameters of the tractor–collector system, the analytical overturning angle was 30.5°. The average overturning angle difference between the simulation and validation was 5°, and the difference between loaded and unloaded conditions was 2°. For 0, 45, and 90° folding positions of the conveyor belt, overturning angles increased and varied from 0.5 to 1°. The vibration level was greater under the unloaded conditions and increased with an increase in the conveyor speed. Vibrations under the loaded condition (0.37~0.48 ms−2) satisfied the ISO (International Organization for Standardization) standard (except the first conveyor belt). According to the PSD analysis, high magnitude peaks (>25 dB) appeared frequently in all directions, which indicates a high possibility of damage to the first conveyor belt. This study provides useful information for improving the safety and durability of agricultural machinery for uneven and sloped field conditions.
Challenges of Machine Learning Applied to Safety-Critical Cyber-Physical Systems
Authors: Pereira Ana , Thomas Carsten
Published: November 2020

Machine Learning (ML) is increasingly applied for the control of safety-critical Cyber-Physical Systems (CPS) in application areas that cannot easily be mastered with traditional control approaches, such as autonomous driving. As a consequence, the safety of machine learning became a focus area for research in recent years. Despite very considerable advances in selected areas related to machine learning safety, shortcomings were identified on holistic approaches that take an end-to-end view on the risks associated to the engineering of ML-based control systems and their certification. Applying a classic technique of safety engineering, our paper provides a comprehensive and methodological analysis of the safety hazards that could be introduced along the ML lifecycle, and could compromise the safe operation of ML-based CPS. Identified hazards are illustrated and explained using a real-world application scenario—an autonomous shop-floor transportation vehicle. The comprehensive analysis presented in this paper is intended as a basis for future holistic approaches for safety engineering of ML-based CPS in safety-critical applications, and aims to support the focus on research onto safety hazards that are not yet adequately addressed.
Mathematical Model of Fractional Duffing Oscillator with Variable Memory
Authors: Kim Valentine , Parovik Roman
Published: November 2020

The article investigates a mathematical model of the Duffing oscillator with a variable fractional order derivative of the Riemann–Liouville type. The study of the model is carried out using a numerical scheme based on the approximation of the fractional derivative of the Riemann–Liouville type by a discrete analog—the fractional derivative of Grunwald–Letnikov. The adequacy of the numerical scheme is verified using specific examples. Using a numerical algorithm, oscillograms and phase trajectories are constructed depending on the values of the model parameters. Chaotic regimes of the Duffing fractional oscillator are investigated using the Wolf–Bennetin algorithm. The forced oscillations of the Duffing fractional oscillator are investigated using the harmonic balance method. Analytical formulas for the amplitude-frequency, phase-frequency characteristics, and also the quality factor are obtained. It is shown that the fractional Duffing oscillator possesses different modes: regular, chaotic, multi-periodic. The relationship between the order of the fractional derivative and the quality factor of the oscillatory system is established.
Hereditary Oscillator Associated with the Model of a Large-Scale αω-Dynamo
Authors: Vodinchar Gleb
Published: November 2020

Cosmic magnetic fields possess complex time dynamics. They are characterized by abrupt polarity changes (reversals), fluctuations of fixed polarity, bursts and attenuations. These dynamic conditions can replace each other, including both regular and chaotic components. Memory in dynamo systems manifests itself in a feedback mechanism when a strong magnetic field begins to change the properties of turbulent flows. A hereditary oscillator can be the simplest model of such complex oscillatory systems with memory. The article suggests the construction of such oscillator by means of two-mode approximation of magnetic field components in the αω-dynamo model. The hereditary member describes the suppression of a field turbulent generator by magnetic helicity and determines the shape of oscillator potential. The article describes the implicit difference scheme for numerical research of oscillator. It also describes the results of numerical simulation for two cases—instantaneous feedback and delay in feedback. The results of simulation are interpreted in terms of oscillator theory. It is shown that the observed dynamic regimes in the model go well with the change of potential shape.
Chemonucleolysis with Chondroitin Sulfate ABC Endolyase for Treating Lumbar Disc Herniation: Exploration of Prognostic Factors for Good or Poor Clinical Outcomes
Authors: Ishibashi Katsuhiko , Fujita Muneyoshi , Takano Yuichi , Iwai Hiroki , Inanami Hirohiko , Koga Hisashi
Published: November 2020

Background and Objectives: Chondroitin sulfate ABC endolyase (condoliase) was launched as a new drug for chemonucleolysis in 2018. Few studies assessed its clinical outcomes, and many important factors remain unclear. This study aimed to clarify the preoperative conditions in which condoliase could be highly effective. Materials and Methods: Of 47 patients who received condoliase, 34 were enrolled in this study. The mean age of the patients was 33 years. The average duration since the onset of disease was 8.6 months. We evaluated patients’ low back and leg pain using a numerical rating scale (NRS) score at two time points (before therapy and 3 months after therapy). We divided the patients into two groups (good group (G): NRS score improvement ≥ 50%, poor group (P): NRS score improvement < 50%). The parameters evaluated were age, disease duration, body mass index (BMI), and positive or negative straight leg raising test results. In addition, the loss of disc height and preoperative radiological findings were evaluated. Results: In terms of low back and leg pain, the G group included 9/34 (26.5%) and 21/34 (61.8%) patients, respectively. Patients’ age (low back pain G/P, 21/36.5 years) was significantly lower in the G group for low back pain (p = 0.001). High-intensity change in the protruded nucleus pulposus (NP) and spinal canal occupancy by the NP ≥ 40% were significantly high in those with leg pain in the G groups (14/21, p = 0.04; and 13/21, p = 0.03, respectively). Conclusions: The efficacy of improvement in leg pain was significantly correlated with high-intensity change and size of the protruded NP. Condoliase was not significantly effective for low back pain but could have an effect on younger patients.
Recent Advancements in Metallic Glasses
Authors: Khonik Vitaly A.
Published: November 2020

Investigations of the structure and properties of metallic glasses constitute a subject of unabated interest [...]
Microstructure Development in Additive Friction Stir-Deposited Cu
Authors: Priedeman Jonathan L. , Phillips Brandon J. , Lopez Jessica J. , Tucker Roper Brett E. , Hornbuckle B. Chad , Darling Kristopher A. , Jordon J. Brian , Allison Paul G. , Thompson Gregory B.
Published: November 2020

This work details the additive friction stir-deposition (AFS-D) of copper and evaluation of its microstructure evolution and hardness. During deposition, a surface oxide is formed on the deposit exterior. A very fine porosity is formed at the substrate–deposit interface. The deposit (four layers of 1 mm nominal height) is otherwise fully dense. The grains appear to have recrystallized throughout the deposit with varying levels of refinement. The prevalence of twinning was found to be dependent upon the grain size, with larger local grain sizes having a higher number of twins. Vickers hardness measurements reveal that the deposit is softer than the starting feedstock. This result indicates that grain refinement and/or higher twin densities do not replace work hardening contributions to strengthen Cu processed by additive friction stir-deposition.
Effect of Cavitation Erosion Wear, Vibration Tumbling, and Heat Treatment on Additively Manufactured Surface Quality and Properties
Authors: Grigoriev Sergey N. , Metel Alexander S. , Tarasova Tatiana V. , Filatova Anastasia A. , Sundukov Sergey K. , Volosova Marina A. , Okunkova Anna A. , Melnik Yury A. , Podrabinnik Pavel A.
Published: November 2020

The paper is devoted to researching various post-processing methods that affect surface quality, physical properties, and mechanical properties of laser additively manufactured steel parts. The samples made of two types of anticorrosion steels—20kH13 (DIN 1.4021, X20Cr13, AISI 420) and 12kH18N9T (DIN 1.4541, X10CrNiTi18-10, AISI 321) steels—of martensitic and austenitic class were subjected to cavitation abrasive finishing and vibration tumbling. The roughness parameter Ra was reduced by 4.2 times for the 20kH13 (X20Cr13) sample by cavitation-abrasive finishing when the roughness parameter Ra for 12kH18N9T (X10CrNiTi18-10) sample was reduced by 2.8 times by vibratory tumbling. The factors of cavitation-abrasive finishing were quantitatively evaluated and mathematically supported. The samples after low tempering at 240 °C in air, at 680 °C in oil, and annealing at 760 °C in air were compared with cast samples after quenching at 1030 °C and tempering at 240 °C in air, 680 °C in oil. It was shown that the strength characteristics increased by ~15% for 20kH13 (X20Cr13) steel and ~20% for 12kH18N9T (X10CrNiTi18-10) steel than for traditionally heat-treated cast samples. The wear resistance of 20kH13 (X20Cr13) steel during abrasive wear correlated with measured hardness and decreased with an increase in tempering temperatures.
The Acquisition of Multidrug-Resistant Bacteria in Patients Admitted to COVID-19 Intensive Care Units: A Monocentric Retrospective Case Control Study
Authors: Bogossian Elisa G. , Taccone Fabio S. , Izzi Antonio , Yin Nicolas , Garufi Alessandra , Hublet Stephane , Njimi Hassane , Ego Amedee , Gorham Julie , Byl Baudouin , Brasseur Alexandre , Hites Maya , Vincent Jean-Louis , Creteur Jacques , Grimaldi David
Published: November 2020

Whether the risk of multidrug-resistant bacteria (MDRB) acquisition in the intensive care unit (ICU) is modified by the COVID-19 crisis is unknown. In this single center case control study, we measured the rate of MDRB acquisition in patients admitted in COVID-19 ICU and compared it with patients admitted in the same ICU for subarachnoid hemorrhage (controls) matched 1:1 on length of ICU stay and mechanical ventilation. All patients were systematically and repeatedly screened for MDRB carriage. We compared the rate of MDRB acquisition in COVID-19 patients and in control using a competing risk analysis. Of note, although we tried to match COVID-19 patients with septic shock patients, we were unable due to the longer stay of COVID-19 patients. Among 72 patients admitted to the COVID-19 ICUs, 33% acquired 31 MDRB during ICU stay. The incidence density of MDRB acquisition was 30/1000 patient days. Antimicrobial therapy and exposure time were associated with higher rate of MDRB acquisition. Among the 72 SAH patients, 21% acquired MDRB, with an incidence density was 18/1000 patient days. The septic patients had more comorbidities and a greater number of previous hospitalizations than the COVID-19 patients. The incidence density of MDRB acquisition was 30/1000 patient days. The association between COVID-19 and MDRB acquisition (compared to control) risk did not reach statistical significance in the multivariable competing risk analysis (sHR 1.71 (CI 95% 0.93–3.21)). Thus, we conclude that, despite strong physical isolation, acquisition rate of MDRB in ICU patients was at least similar during the COVID-19 first wave compared to previous period.
Crystal Structure Refinements of Four Monazite Samples from Different Localities
Authors: Zaman M. Mashrur , Antao Sytle M.
Published: November 2020

This study investigates the crystal chemistry of monazite (APO4, where A = Lanthanides = Ln, as well as Y, Th, U, Ca, and Pb) based on four samples from different localities using single-crystal X-ray diffraction and electron-probe microanalysis. The crystal structure of all four samples are well refined, as indicated by their refinement statistics. Relatively large unit-cell parameters (a = 6.7640(5), b = 6.9850(4), c = 6.4500(3) Å, β = 103.584(2)°, and V = 296.22(3) Å3) are obtained for a detrital monazite-Ce from Cox’s Bazar, Bangladesh. Sm-rich monazite from Gunnison County, Colorado, USA, has smaller unit-cell parameters (a = 6.7010(4), b = 6.9080(4), c = 6.4300(4) Å, β = 103.817(3)°, and V = 289.04(3) Å3). The a, b, and c unit-cell parameters vary linearly with the unit-cell volume, V. The change in the a parameter is large (0.2 Å) and is related to the type of cations occupying the A site. The average  distances vary linearly with V, whereas the average  distances are nearly constant because the PO4 group is a rigid tetrahedron.
The Newly Discovered Neoproterozoic Aillikite Occurrence in Vinoren (Southern Norway): Age, Geodynamic Position and Mineralogical Evidence of Diamond-Bearing Mantle Source
Authors: Zozulya Dmitry R. , Kullerud Kåre , Ribacki Enrico , Altenberger Uwe , Sudo Masafumi , Savchenko Yevgeny E.
Published: November 2020

During the period 750–600 Ma ago, prior to the final break-up of the supercontinent Rodinia, the crust of both the North American Craton and Baltica was intruded by significant amounts of rift-related magmas originating from the mantle. In the Proterozoic crust of Southern Norway, the 580 Ma old Fen carbonatite-ultramafic complex is a representative of this type of rocks. In this paper, we report the occurrence of an ultramafic lamprophyre dyke which possibly is linked to the Fen complex, although 40Ar/39Ar data from phenocrystic phlogopite from the dyke gave an age of 686 ± 9 Ma. The lamprophyre dyke was recently discovered in one of the Kongsberg silver mines at Vinoren, Norway. Whole rock geochemistry, geochronological and mineralogical data from the ultramafic lamprophyre dyke are presented aiming to elucidate its origin and possible geodynamic setting. From the whole-rock composition of the Vinoren dyke, the rock could be recognized as transitional between carbonatite and kimberlite-II (orangeite). From its diagnostic mineralogy, the rock is classified as aillikite. The compositions and xenocrystic nature of several of the major and accessory minerals from the Vinoren aillikite are characteristic for diamondiferous rocks (kimberlites/lamproites/UML): Phlogopite with kinoshitalite-rich rims, chromite-spinel-ulvöspinel series, Mg- and Mn-rich ilmenites, rutile and lucasite-(Ce). We suggest that the aillikite melt formed during partial melting of a MARID (mica-amphibole-rutile-ilmenite-diopside)-like source under CO2 fluxing. The pre-rifting geodynamic setting of the Vinoren aillikite before the Rodinia supercontinent breakup suggests a relatively thick SCLM (Subcontinental Lithospheric Mantle) during this stage and might indicate a diamond-bearing source for the parental melt. This is in contrast to the about 100 Ma younger Fen complex, which were derived from a thin SCLM.
Zircon U–Pb Geochronology, Geochemistry and Geological Significance of the Anisian Alkaline Basalts in Gejiu District, Yunnan Province
Authors: Shang Zhi , Chen Yongqing
Published: November 2020

The Gejiu Anisian alkaline basalts (GAAB), distributed in the southern part of the Emeishan large igneous province (ELIP), are crucial to understand the tectonomagmatic activity during the Triassic. Geochronological, geochemical, and Sr-Nd-Pb isotopic analyses were systematically applied to explore the origin, petrogenesis, and tectonic setting of the GAAB, and how they relate to the ELIP. Zircon U-Pb dating set the eruption date at 244 Ma. Most of the samples belonged to alkaline basalts and had high TiO2 (2.14–3.23 wt.%) and MgO (4.43–19.58 wt.%) contents. Large ion lithophile elements (LILEs) were enriched relative to high field strength elements (HFSEs). The rare earth elements (REEs) and trace element signatures in the normalized diagrams were similar to oceanic island basalts (OIB) and Emeishan high-Ti basalts. These samples had consistent Sr-Nd isotope compositions: the initial 87Sr/86Sr values ranged from 0.7044 to 0.7048 and εNd(t) = 3.25–4.92. The Pb isotopes were more complex, the (206Pb/204Pb)t, (207Pb/204Pb)t, (208Pb/204Pb)t ratios were 17.493–18.197, 15.530–15.722, and 37.713–38.853, respectively. Our results indicate that the GAAB originated from the deeper enriched mantle with 5% to 15% partial melting of garnet lherzolite and a segregation depth of 2 to 4 GPa (60–120 km). During the formation of the GAAB, clinopyroxene and Ti-Fe oxides were fractionally crystallized with insignificant crustal contamination. The GAAB were formed in a extensional regime that was related to the Gejiu-Napo rift event in the Triassic.
Chemical Characterization of Three Accessions of Brassica juncea L. Extracts from Different Plant Tissues
Authors: Oulad El Majdoub Yassine , Alibrando Filippo , Cacciola Francesco , Arena Katia , Pagnotta Eleonora , Matteo Roberto , Micalizzi Giuseppe , Dugo Laura , Dugo Paola , Mondello Luigi
Published: November 2020

Indian mustard or Brassica juncea (B. juncea) is an oilseed plant used in many types of food (as mustard or IV range salad). It also has non-food uses (e.g., as green manure), and is a good model for phytoremediation of metals and pesticides. In recent years, it gained special attention due to its biological compounds and potential beneficial effects on human health. In this study, different tissues, namely leaves, stems, roots, and flowers of three accessions of B. juncea: ISCI 99 (Sample A), ISCI Top (Sample B), and “Broad-leaf” (Sample C) were analyzed by HPLC-PDA/ESI-MS/MS. Most polyphenols identified were bound to sugars and phenolic acids. Among the three cultivars, Sample A flowers turned were the richest ones, and the most abundant bioactive identified was represented by Isorhamnetin 3,7-diglucoside (683.62 µg/100 mg dry weight (DW) in Sample A, 433.65 µg/100 mg DW in Sample B, and 644.43 µg/100 mg DW in Sample C). In addition, the most complex samples, viz. leaves were analyzed by GC-FID/MS. The major volatile constituents of B. juncea L. leaves extract in the three cultivars were benzenepropanenitrile (34.94% in Sample B, 8.16% in Sample A, 6.24% in Sample C), followed by benzofuranone (8.54% in Sample A, 6.32% in Sample C, 3.64% in Sample B), and phytone (3.77% in Sample B, 2.85% in Sample A, 1.01% in Sample C). The overall evaluation of different tissues from three B. juncea accessions, through chemical analysis of the volatile and non-volatile compounds, can be advantageously taken into consideration for future use as dietary supplements and nutraceuticals in food matrices.
Effect of Xylo-Oligosaccharides Supplementation by Drinking Water on the Bone Properties and Related Calcium Transporters in Growing Mice
Authors: Gao Hang , Zhou Zhenlei
Published: November 2020

Xylo-oligosaccharides (XOS), non-digestible oligosaccharides, have the potential to regulate intestinal microorganisms, and thus, improve host health, but little evidence exists for the prebiotic effects on bone health. This study evaluates the dose-response effect of XOS supplementation on bone properties, the morphology of the intestine, cecum pH, and cecum wall weight, as well as the related calcium transporters. Ninety-six 28-day-old male mice were randomized into one of four groups, fed the same commercial diet, and given different types of deionized water containing 0, 1, 2, or 4% XOS by concentration for 30 days. Eight mice were randomly selected to accomplish particular tasks every 10 days. No significant differences in serum Ca and P levels and growth performance were observed among the four studied groups. XOS intervention significantly decreased cecum pH and increased cecum wall weight in a dose-dependent manner. At the late growth stage, compared with 0% XOS, the bone mineral density (BMD) and bone-breaking strength in 4% XOS were significantly higher. The bone crystallinity with 4% XOS, measured by Raman spectrum, was significantly enhanced compared to that with 0% XOS during later growth. The villus height and villus height to crypt depth (VH:CD) were enhanced with an increase of XOS concentration during the later stage of growth. The expression of transient receptor potential vanillin receptor 6 (TRPV6) and Na+/Ca2+ exchanger 1 (NCX1) in the duodenum were enhanced by XOS supplementation. XOS exerted a positive influence on bone properties by decreasing the cecum pH, increasing the cecum wall and villus structure, and upregulating the expression of related calcium transporters.
Distinct Postprandial Bile Acids Responses to a High-Calorie Diet in Men Volunteers Underscore Metabolically Healthy and Unhealthy Phenotypes
Authors: Lamaziere Antonin , Rainteau Dominique , KC Pukar , Humbert Lydie , Gauliard Emilie , Ichou Farid , Ponnaiah Maharajah , Bouby Nadine , Salem Joe-Elie , Mallet Jean-Maurice , Guerin Maryse , Lesnik Philippe
Published: November 2020

Bile acids (BAs) regulate dietary lipid hydrolysis and absorption in the proximal intestine. Several studies have highlighted a determinant role of circulating levels and/or metabolism of BAs in the pathogenesis of major cardiometabolic diseases. Whether changes in BA profiles are causative or are consequence of these diseases remains to be determined. Healthy male volunteers (n = 71) underwent a postprandial exploration following consumption of a hypercaloric high fat typical Western meal providing 1200 kcal. We investigated variations of circulating levels of 28 BA species, together with BA synthesis marker 7α-hydroxy-4-cholesten-3-one (C4) over an approximately diurnal 12 h period. Analysis of BA variations during the postprandial time course revealed two major phenotypes with opposite fluctuations, i.e., circulating levels of each individual species of unconjugated BAs were reduced after meal consumption whereas those of tauro- and glyco-conjugated BAs were increased. By an unbiased classification strategy based on absolute postprandial changes in BA species levels, we classified subjects into three distinct clusters; the two extreme clusters being characterized by the smallest absolute changes in either unconjugated-BAs or conjugated-BAs. Finally, we demonstrated that our clustering based on postprandial changes in BA profiles was associated with specific clinical and biochemical features, including postprandial triglyceride levels, BMI or waist circumference. Altogether, our study reveals that postprandial profiles/patterns of BAs in response to a hypercaloric high fat challenge is associated with healthy or unhealthy metabolic phenotypes that may help in the early identification of subjects at risk of developing metabolic disorders.
Assessing the Intestinal Permeability and Anti-Inflammatory Potential of Sesquiterpene Lactones from Chicory
Authors: Matos Melanie S. , Anastácio José D. , Allwood J. William , Carregosa Diogo , Marques Daniela , Sungurtas Julie , McDougall Gordon J. , Menezes Regina , Matias Ana A. , Stewart Derek , Santos Cláudia Nunes dos
Published: November 2020

Cichorium intybus L. has recently gained major attention due to large quantities of health-promoting compounds in its roots, such as inulin and sesquiterpene lactones (SLs). Chicory is the main dietary source of SLs, which have underexplored bioactive potential. In this study, we assessed the capacity of SLs to permeate the intestinal barrier to become physiologically available, using in silico predictions and in vitro studies with the well-established cell model of the human intestinal mucosa (differentiated Caco-2 cells). The potential of SLs to modulate inflammatory responses through modulation of the nuclear factor of activated T-cells (NFAT) pathway was also evaluated, using a yeast reporter system. Lactucopicrin was revealed as the most permeable chicory SL in the intestinal barrier model, but it had low anti-inflammatory potential. The SL with the highest anti-inflammatory potential was 11β,13-dihydrolactucin, which inhibited up to 54% of Calcineurin-responsive zinc finger (Crz1) activation, concomitantly with the impairment of the nuclear accumulation of Crz1, the yeast orthologue of human NFAT.
Detection of Neospora caninum Infection in Aborted Equine Fetuses in Israel
Authors: Leszkowicz Mazuz Monica , Mimoun Lea , Schvartz Gili , Tirosh-Levy Sharon , Savitzki Igor , Edery Nir , Blum Shlomo E. , Baneth Gad , Pusterla Nicola , Steinman Amir
Published: November 2020

In horses, Neospora caninum and Neospora hughesi have been associated with fetal loss, and neurological disease, respectively. This study investigated the role of Neospora spp. infection in equine abortion in Israel. The presence of anti-Neospora spp. antibodies was evaluated in 31 aborting mares by indirect fluorescent antibody test (IFAT) and the presence of parasite DNA in their aborted fetuses was evaluated by polymerase chain reaction (PCR), using two target loci (ITS1 and Nc5). The seroprevalence found in aborting mares was 70.9% and the prevalence by DNA detection in the aborted fetuses was 41.9%. Transplacental transmission from positive mares to their fetuses was 45.4% (10/22), while 33.3% (3/9) of fetuses of seronegative mares also tested positive for Neospora. The use of two PCR targets improved the sensitivity of parasite detection, and positive samples were identified by sequence analyses as N. caninum. These finding suggest that N. caninum could be a significant cause of abortion in horses, and that transplacental transmission in horses is an important way of transmission of N.caninum. The results presented here demonstrated the necessity to use several tests concurrently, including serological and molecular assays in order to confirm the involvement of Neospora in mare abortions.
Availability of Guanitoxin in Water Samples Containing Sphaerospermopsis torques-reginae Cells Submitted to Dissolution Tests
Authors: Fernandes Kelly , Ferraz Humberto , Vereau Fanny , Pinto Ernani
Published: November 2020

Guanitoxin (GNT) is a potent neurotoxin produced by freshwater cyanobacteria that can cause the deaths of wild and domestic animals. Through reports of animal intoxication by cyanobacteria cells that produce GNT, this study aimed to investigate the bio-accessibility of GNT in simulated solutions of the gastrointestinal content in order to understand the process of toxicosis promoted by GNT in vivo. Dissolution tests were conducted with a mixture of Sphaerospermopsis torques-reginae (Cyanobacteria; Nostocales) cultures (30%) and gastrointestinal solutions with and without proteolytic enzymes (70%) at a temperature of 37 °C and rotation at 100 rpm for 2 h. The identification of GNT was performed by LC-QqQ-MS/MS through the transitions [M + H]+m/z 253 > 58 and [M + H]+m/z 253 > 159, which showed high concentrations of GNT in simulated gastric fluid solutions (p-value < 0.001) in comparison to simulated solutions of intestinal content. The gastric solution with pepsin promoted the stability of GNT (p-value < 0.05) compared to the simulated solution of gastric fluid at the same pH without the enzyme. However, the results showed that GNT is also available in intestinal fluids for a period of 2 h, and solutions containing the pancreatin enzyme influenced the bio-accessibility of the toxin more compared to the intestinal medium without enzyme (p-value < 0.05). Therefore, the bio-accessibility of the toxin must be considered both in the stomach and in the intestine, and may help in the diagnosis and prediction of exposure and risk in vivo through the oral ingestion of GNT-producing cyanobacteria cells.