bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Platinum Complex as Telomeric G-Quadruplex Binders
Authors: Luo Xu Jian , Qin Qi Pin , Li Yu Lan , Liu Yan Cheng
Published: September 2013

A new platinum (II) complex has been synthesized and characterized by IR, NMR, ESI-MS and element analysis. The affinities of the complex toward telomeric G-quadruplex DNA [Htel-21] has been investigated by CD, UVVis. The results revealed that the complex can induce and stabilizes the antiparallel telomeric G-quadruplex DNA, and bind very strongly to G-quadruplex DNA. The inhibition ratio of the complex was screened against four cancer cell lines in comparison to cisplatin and it showed a higher activity than cisplatin.
The Semi-Synthesis and Cytotoxic Activity of Trametenolic Acid B Derivatives
Authors: Zhang Qiao Yin , Ding Ming Ruo , Zhai Wei Chen , Yao Yuan , Zhou Yan Mei , Wang Jun Zhi
Published: September 2013

The trametenolic acid B was a bioactive lanostane-type triterpenoid inTrametes lactinea(Berk.) Pat with. Five novel trametenolic acid B derivatives were synthesized, characterized and evaluated the cytotoxicity against MDA-MB-231 cells with the aim of obtaining better anti-tumtor agents. The compound IV exhibited significantly cytotoxicity activities with IC50value of 22.39 μM.
Studies on the Chemical Constituents of Fritillaria monanth
Authors: Luo Yong Ming , Li Fei , Liu Hong Ning , Zhu Wei Feng , Yan Dong Mei
Published: September 2013

From the fresh bulbs of Fritillaria monatha Migo., ten compounds were isolated and identified as peimine (1), peiminine (2), isovertifine (3), pengbeimine A(4), pengbeimine B(5), pengbeimine C(6), pengbeimine D(7),stearic acid (8), daucosterol (9) and campesterol (10). Their structures were determined by NMR, MS spectral data.
Researches on the Structure and Antioxidant Activity of Apigenin-La (III) Complex
Authors: Wen Feng Qiu , Pan Lang Sheng , Lei Lei
Published: September 2013

La (III) complex with apigenin was synthesized and characterized by UV, IR, 1HNMR and thermal analysis. The scavenging activity of the complex on superoxide anion radical (O2-·) and hdroxyl radical (·OH) was also investigated by improved pyrogallic acid way and ortho-oxybenzoic acid way. The results show that the complex had remarkable scavenging effects on O2-· and ·OH, and its scavenging effect on free radicals was better than apigenin.
Effect of Plant Compound Curcumin on the Expression of SR-BI and ABCA1 in Hippocampal Neurons of APPswe/PS1dE9 Double Transgenic Mice
Authors: Sun Jie Yun , Teng Zhi Peng , Wang Chen , Tian Ming Yuan , Zhang Xiong , Li Yu
Published: September 2013

Disorder of cholesterol metabolism plays important roles in pathogenesis of Alzheimer’s disease (AD). Plant compound curcumin has been reported to decrease Aβ deposition and cholesterol in serum, while the detailed mechanism is still unknown. To investigate the effect of curcumin on the cholesterol metabolism in Alzheimer’s disease, APPswe/PS1dE9 double transgenic mice were fed with 500ppm of curcumin every day for six months. Immunohistochemistryresults showed that the expression of ATP-binding cassette transporter A1 (ABCA1) in hippocampal neurons was increased significantly, whereas the expression of scavenger receptor class B type I (SR-BI) was not detected. These findings suggest that curcumin may promote cholesterol efflux via ABCA1 transmembrane-transport system rather than SR-BI in neurons of AD.
Mechanisms of Natural Food Dyes Curcumin on Regulation of HO-1/HO-2 and Inhibition of Aβ-Heme Compound in Alzheimer's Disease
Authors: Zhang Xiong , Lü Yang , Sun Jie Yun , Tang Yong , Yu Li
Published: September 2013

More and more studies have reported that β-amyloid (Aβ)-induced oxidative stress and protein metabolism disorders along with their interactions are likely to be the key factors to the pathogenesis of Alzheimers disease (AD). Heme oxygenase (HO) is one member of stress responsive enzyme super family and is a joint of many hypothesis for AD, while oxidative stress, iron metabolism disorders and Aβ deposition are closely related with HO. Therefore, HO is expected to become a therapeutic target for AD. HO-1 and HO-2 are the main members of HO family, and keep dynamic balance. In normal aging brain tissues, the expression of HO-2 is high, and that of HO-1 is low; while in the cerebral cortex and hippocampus of AD patients, the expression of HO-1 is significantly increased. This phenomenon indicates that HO-1 has a protective effect to the neurons from the oxidative stress. Furthermore, heme and Aβ could form Aβ-heme compound, which is a peroxidase complex, which increase the oxidative damage to neurons. Recently, Curcumin has been shown cytoprotective properties by inducing HO-1 and by preventing the formation of Aβ-heme in neurons; however, the underlying mechanisms are still unclear to date. Therefore, there has been great interest in understanding the molecular mechanisms based on curcumin acts on.
Indirect Determination of Methimazole in Pharmaceutical Sample by Discoloration Spectrophotometry Using Fe (III)-Tiron System
Authors: Tu Chang Qing , Wen Xin Rong
Published: September 2013

A novel method for the indirect determination of methimazole by discoloration spectrophotometry using Fe (Ш)-tiron system has been established.The various effect factors on the determination of methimazole by discoloration spectrophotometry using tiron-Fe (Ш) system were investigated in detail. The results showed that by controlling pH=2.8,Fe (Ш) could be reduced to Fe (II) by hydrosulfuryl (-SH) in methimazole molecule,and then using tiron as chromogenic reagent of Fe (Ш),and the content of methimazole was determinated indirectly through determinating the surplus content of Fe (Ш) in the system.The maximum absorption wavelength of chromogenic system was 663 nm, good linear relationship was obtained between A and the concentration of methimazole in the range of 3.2~12.8μg·mL-1,the equation of the linear regression was A=-0.1849+57.277ρ (mg·mL-1),with a linear correlation coefficient was 0.9998.This proposed method had been successfully applied to determinate of methimazole in real pharmaceutical.
A Prophase Study on Applying Conia-Ene Reation in Constructing bicyclo [3, 3, 1] Nonane Ring
Authors: Chen Shi Peng , Zhang Jian Ting , Liu Dong Wang , Tang Lin Jun , Feng Jun Ming , Wang Xiao Ji , Huang Shuang Ping
Published: September 2013

The bicyclo [3, 3, 1] nonane ring system is the main structural feature in the lycopodium alkaloids. There are many methods to build the [3, 3, 1] bridged ring system, Herein, we designed to use indium Lewis acid which can activate the triple bond to construct [3, 3, 1] bridged ring system via Conia-ene reaction, and described a prophase study of applying Conia-ene reaction to construct such bicycle [3, 3, 1] nonane ring.
Synthetic Study of 1, 2, 3-Tri-O-Acetyl-5-Deoxy-D-Ribofuranose
Authors: Zhang Jian Ting , Chen Shi Peng , Feng Jun Min , Liu Dong Wang , Tang Lin Jun , Wang Xiao Ji , Huang Shuang Ping
Published: September 2013

A practical synthetic route towards 1,2,3-tri-O-acetyl-5-deoxy-D-ribofuranose from D-ribose is described, which started from D-ribose and proceeded through ketalization,esterification,reduction,hydrolysis and acetylation in more than 30% overall yield.
Study on Synthesis of (S)-methyl 3-(((benzyloxy)carbonyl) amino)-7-hydroxyheptanoate
Authors: Feng Jun Min , Chen Shi Peng , Tang Lin Jun , Zhang Jian Ting , Wang Xiao Ji , Huang Shuang Ping
Published: September 2013

(S)-methyl 3-(((benzyloxy) carbonyl) amino)-7-hydroxyheptanoate (7), a fragment of a novel cytotoxic cyclodepsipeptide onchidin[, which shows moderate cytotoxic activity, was synthesized through six steps. The key stereoselective synthesis of beta-amino acid derivatives (7) was achieved by the Arndt-Eistert homologation reaction[2,3] and Wolff rearrangement reaction[4].
Synthesis and Characterization of Novel Roflumilast Analogues
Authors: Si Yu Hui , Sun Gang Chun , Song Ge , Mai Wen Peng , Li Zhi Cheng
Published: September 2013

A series of new roflumilast analogues with Crown ether structures were designed and synthesized. Crown-ether-type benzaldehydes were first prepared from 3,4-dihydroxybenzaldehyde with 1-bromo-3-chloropropane and 1-chloro-2-(2-chloroethoxy) ethane respectively, then after oxidation, acylation and amidation as the last step with 3,5-dichloropyridin-4-amine the roflumilast analogues were obtained. These compounds were characterised by1H NMR, IR, ESI-MS and elemental analysis.
Research in Benzimidazole Compounds as Antibacterial Agents
Authors: Meng Jiang Ping , Hu Cheng Bo , Wu Fei Yue
Published: September 2013

Benzimidazole compounds are known to possess varied biological activities and so far various types of benzimidazole drugs have extensively been used in clinic. Benzimidazole compounds as a antibacterial agents has been a quite rapidly developing and is gradually becoming a relatively independent scientific area. In this paper we presented the recent advances of benzimidazole compounds as antibacterial agents. The perspective of the foreseeable future and potential application of benzimidazole as antibacterial agents are also presented.
Analysis of Miconazole Nitrate, Econazole Nitrate, Acetic Acid Chloride with the Use of Ultraviolet Spectrophotometry and Chemometrics
Authors: Lei Yan Hong
Published: September 2013

A direct and novel ultraviolet spectrophotometry method for analysis of complex samples was researched and developed with the use of chemometrics, using the Miconazole nitrate, econazole nitrate,acetic acid chloride as example samples. The spectra (range: λ=240~280 nm were processed using Partial least squares (PLS).The ultraviolet spectrophotometry data of the samples were also used to evaluate the samples’ quantitative composition.It was found that the samples were clearly discriminated.A PLS model was developed for simultaneous analysis of the three analytes . The satisfactory prediction results for synthetic samples were obtained by Partial least squares (PLS) as calibration methods (relative prediction error RPET =4.23%).
Study on Anti-Osteoporosis of Compound Pearl Protein Polypeptide
Authors: Guo Jian , Liao Jie , Li Yan Ping , Song Wen Dong , Liu Jian She
Published: September 2013

Compound pearl protein polypeptide (CPPP) was investigated by MTT method as well as detecting alkaline phosphatase (ALP) and mineralized nodes in osteoblasts from SD rats. In vivo the rat models of osteoporosis were induced by ovariectomy and treated with three doses of CPPP (5.0mg.kg-1.d-1, 20.0mg.kg-1.d-1, and 80.0mg.kg-1.d-1). The serum levels of estradiol (E2), osteocalcin (BGP), transforming growth factors (TGF-β1) and ALK, together with the bone mineral density (BMD) and the contents of calcium (Ca) and phosphorus (P) in 24h urine, were detected. The results show that CPPP can enhance cell proliferation, ALK activity and the number of mineralized nodes in rats osteoblasts. CPPP significantly increase the serum level of E2, TGF-β1, and BMD, and decrease the serum level of ALP, BGP and the contents of Ca, P in 24h urine. So, CPPP can increase bone density, and thus it is conductive to osteoporosis prevention and treatment of the elderly.
Comparative Anti-Tumor Activity Study of Tartary Buckwheat Flavonoids and Amphibian Peptides
Authors: Zhou Xiao Li , Meng Xiao Xiao , Wang Qing , Zhou Yi Ming , Li Zong Jie
Published: September 2013

In China, many kinds of natural products extracted from plant and animal were used to treat cancer. Flavonoids extracted from tartary buckwheat and skin peptide purified from rufous-spotted torrent frog had respectively been proved to have anti-tumor activity. In present study, both tartary buckwheat flavonoids and amphibian frog peptides were applied for cell proliferation inhibitory activity against human gastric cancer MGC 80-3 cells using MTT assay. According to the data, the buckwheat flavonoids exhibited much stronger anti-tumor activity than amphibian peptides, and the complex mixture of this two kinds of bioactive compounds still possess anti-tumor activity.
Chemical Constituents from Marine Streptomycete sp. S15
Authors: Jiang Kun , Yang Sheng Xiang
Published: September 2013

Six known compounds were isolated from fermentation broth extract of the marine Streptomycete sp. S15. Their structures were identified to be cyclo-(L-Pro-L-Ser) (1), tryptophan (2), cyclo-(Ala-4-OH-Pro)(3),Linoleic acid (4), anthranilic acid (5) and N-[2-(4-hydroxy-phenyl) ethyacetamide (6) by spectral methods. The brine shrimp bioassay showed the mortality rates of Arbutin to brine shrimp are 75.8% at a concentration of 10μg/mL, indicating Arbutin possessed significant toxicity on brine shrimp.
Optimization of Selenium Yeild by Saccharomyces sp. III Using Statistical Designs
Authors: Sun Na Xin , Zhang Gui Xiang , Liu Yue Hui , Wang Yuan Xiu
Published: September 2013

The objective of the study was to optimize the conditions in a culture medium for the selenium yeild enriched by Saccharomyces sp. III using Plackett-Burman design and Box-Behnken design. The Plackett-Burman multifactorial design was first employed to screen the significant factors in the fermentation for the selenium yeild, and subsequent use of the response surface methodology was further optimized for the selenium yeild by Box-Behnken design. The important factors in the culture medium, identified by the initial screening method of Placket-Burman, were sodium selenite, glucose and the liquid volume. The optimal amounts for maximum selenium yeild were: sodium selenite 15.8 mg/L; glucose 40.2 g/L; the liquid volume 120 mL in 250 mL flask. Using this statistical experimental design, the selenium yeild under optimal condition reached about 1679.32 μg selenium /g dry cell.
Establishment of Micellar Electro Kinetic Chromatography Detection Method for Puerarin in Pueraria Lobata(Willd.)Ohwi
Authors: Liu Xing Rong , Zhu Dao Chen , Chen Yuan Mei
Published: September 2013

Relative to the pharmacopoeia of high performance liquid chromatography (HPLC), establishing detection of puerarin in radix puerariae micellar electro kinetic chromatography (MEKC). Studying on buffer pH, borax concentration, separation voltage, SDS concentration of chromatographic conditions on the puerarin MEKC separation effect of influence, determined the best capillary electrophoresis conditions: test 250 nm wavelength, buffer of pH 9.0 0.1M KH2PO4solution-20 mM borax solution, separation voltage 18 kV, 40 mmol/L SDS concentration, 4.5 min retention time., grained puerarin MEKC standard equation: Y=36.522X-471.9, R2=0.9994(N=5), the puerarin concentration in 550 μg/mL to 750 μg/mL range comes out a good relationship. Get the lowest detection limit with 13.74 μg/mL. Puerarin content of the total isoflavone detected by MEKC was 45.35% that similar to 42.25% detected by HPLC. The method was stable, accurate, sensitive, possessed organic solvent consumption. The research results have applications in puerarin and its product quality evaluation system and promote isoflavonoids detection.
DPPH Radical Scavenging Activity of Kaempferol
Authors: Liu Ping , Gao Yun Tao , Yu Jiao Jiao , Cha Jia Wei , Zhao Dong Mei , Li Yan Hua , Chen Jing Hua
Published: September 2013

The UV-Vis absorption spectrum of kaempferol and DPPH was investigated, the optimum determination wavelength and reaction time for determining the DPPH radical scavenging activity of kaempferol was 517 nm and 30 min, respectively. Kaempferol exhibited strong DPPH radical scavenging activity with a IC50 value of 0.004349 mg·mL-1, which is smaller than that of rutin, indicating that kaempferol has a stronger antioxidant activity than rutin.
Endosymbiotic Association between Methanogens and Hydrogen-Producing Organisms - Hydrogen Hypothesis
Authors: Yin Fang , Zhang Wu Di , Liu Shi Qing , Liu Jing , Huang Zun Xi
Published: September 2013

Many archaebacteria are strictly dependent upon H2for their ATP production. Moreover, for many methanogens (the strictly lithoautotrophic forms), H2O, CO2and acetate are the sole source of both energy and carbon, all of which are waste products of the symbionts anaerobic metabolism. The fact that methanogens associate with hydrogen-producing organisms means endosymbiotic methanogens cling not to free-living eubacteria, but hydrogenosomes themselves in the cytosol of amitochondriate protists. This has given rise to the theory that the eukaryotic cell originated from syntrophic interactions between bacteria and archaea. We call this new hypothesis for the origin of eukaryotic cells as hydrogen hypothesis.
Rapid Preparation of Solid Alcohol
Authors: Du Hai Yan , Fan Jing , Wu Wei Ming
Published: September 2013

This paper investigated several influencing factors for the preparation of solid alcohol, including the amount of raw materials used such as stearic acid and copper nitrate and so on, and the temperature, etc. Through single factor experiments, the main influence factors of preparing solid alcohol were determined. And, on this basis, in view of three remarkable influence factors: the stearic acid amount used, the copper nitrate amount used and the temperature, the orthogonal experiment was carried out. Finally, the optimal preparation conditions were determined as follows: 25ml industrial alcohol, 1.3g stearic acid, 1.2ml 10% copper nitrate solution, at 75°C, pH 8, the cooling way is gradual cooling with no refluxing.
Comparison on the Legal Management on Genetically Modified Food between the Europe and the US and China‘s Legal Reform
Authors: Li Ren Yuan
Published: September 2013

The European Union and the United States have imposed different legal management on the genetically modified food. There are some disadvantages in the legal regime of China concerning the management of GMF which make the regal reform on the issue of GMF become necessary. To ensure food safety, China should take the legal regime of EU as reference and raise its standards for the market access of the GMF. The requirements of traceability and labeling for GMF should also be included in law. A centralized governmental department responsible for the issues of GMF should also be established.
Mathematical Modeling of Drying Kinetics of Salted Otolithes Ruber at the Different Temperature
Authors: Wu Yan Yan , Ren Zhong Yang , Li Lai Hao , Yang Xian Qing , Lin Wan Ling , Zhou Wan Jun , Ma Hai Xia , Deng Jian Chao
Published: September 2013

Salted Otolithes ruber with 80 (±5) g weight on dry basis were dried in the blast electric oven using different temperature (20, 30, 40 and 50°C) until the humidity fell down to 0.6 from 1.23 on dry basis. Drying processes were completed between 20-48h. In this study, experiment values were compared with predicted values obtained from twenty thin layer drying theoretical/ semi-empirical/ empirical equations. Models whose coefficient of correlation (R2) values are highest were chosen to be the best models. According to this, Midillis Model was defined as the most suitable model in which predicted value is closest to experimental data for 20°C level, Weibulls model was for 30°C level, Weibull distributions model was for 40°C level and Alibas model was for 50°C level.
The Nutritional Value and Preliminary Risk Assessment of Major and Trace Elements Accumulated in 8 Edible Seaweeds
Authors: Feng Ting Ting , Chen Hong Bo , Shao Ping , He Jin Zhe
Published: September 2013

16 major and trace elements content of 8 edible seaweeds were determined by ICP-AES after wet digestion to compare the nutritional value and pollution level between Cyanophyta (blue), Chlorophyta (green), Rhodophyta (red), Phaeophyta (brown) species. The concentration changes of trace elements in seaweeds had some differences from four species, but the concentration distribution tendency of trace elements was similar. Major elements ( K,Ca,Na,Mg,P) and functional elements (Fe,Mn,Zn,Cu,Se,Cr) were abundant in Cyanophyta (blue) and Phaeophyta (brown) species, these two species, however, accumulated Al selectively. The pollution levels of toxic elements like As, Pb and Hg in most seaweeds from this study demonstrated that the cultivation aquatic eco- environments were relatively safe. In this paper, Nostoc commune var. Flagelliforme (Cyanophyta) abounds in Fe, Zn and major elements, moreover the concentration of potentially toxic elements were relatively low. The second was Phaeophyta species, especially Laminaria japonica Aresch . Porphyra yezoensis Ueda (Rhodophyta) shows the lowest nutritional value.
Research on the Influencing Factors of Raw Milk Quality and Control
Authors: Chen Jing , Zhao Jing , Zhang Yao Li
Published: September 2013

Introduced quality situation of milk supply, this paper made a sorting and analysis on the factors got from literature review and field research that influence milk supply by using fishbone diagram. The factors are as follows: raising modes and environment of dairy cows; feed; raising management; milking and purchasing; stocking; transportation; Aiming at these factors, this paper suggest to build scaled milk supply base; improve dairy cow raising environment; strengthening feed management; build HACCP quality control system of dairy cow supply and third-party detection mechanism.
Optimization of Fermentation Conditions for Higher Monacolin K Production by Monascus purpureus
Authors: Yu Li Juan , Zhang Hong Xing , Xie Yuan Hong , Ma Si Ming , Liu Hui , Luo Yun Bo
Published: September 2013

Monacolin K is a secondary metabolite of Monascus fermentation with lowering blood fat, cholesterol and blood sugar. The culture substrates and fermentation conditions were optimized by means of L9 (33) orthogonal experiments. The optimized fermentation conditions were studied and concluded: initial moisture content 50%, material thickness 4 cm and fermentation time 13 days. Using rice as substrate, the maximum yield of monacolin K was 2.50 mg/g in the optimized conditions. Using glutinousrice as substrate, the maximum yield of monacolin K was 2.71 mg/g in the optimized conditions. The founding determined that glutinousrice was used as substrate in solid state fermentation by Monascus purpureus to produce monacolinK.
Quasiclassical Trajectory Calculations of Stereodynamics and Product State Distributions in the Reaction of H with OCl
Authors: Yue Xian Fang , Wu Victor Wei Keh , Cheng Jie
Published: September 2013

A first quasiclassical trajectory calculation for the reaction H + OCl OH + Cl, HCl + O has been carried out on the ground 11A' potential energy surface (PES) at the collision energy of 7.6 kcal/mol. The stereodynamics and product state distributions were focused on computation and analysis. It was found that, for both the OH and HCl products, the product rotational alignment and orientation were very weak. Most of the OH products are at lower vibrational levels. The HCl products dominantly populate in the ground vibrational state v'=0. Inverted rotational state distributions were found in each of the vibrational state for both the OH and HCl products. As a consequence, the title reaction takes place dominantly through an indirect mechanism involving a long-lived complex.
Hydrate Formation Conditions of Natural Gas with Different Content of Carbon Dioxide and Inhibitors Screening Studies
Authors: Guo Ping , Shen Xiu Yue , Du Jian Fen , Wang Zhou Hua
Published: September 2013

In order to study the formation conditions of CO2-gas hydrate and screen appropriate inhibitors, DBR hydrate test device was put into use .Under 0~15MPa and -10~38°C ,the production conditions of natural gas hydrate was measured when the content of CO2 in the system was 10%,30%,50%,70%,90% respectively .According to prior work ,methanol and ethylene glycol was selected to be the inhibitors .Different concentrations of inhibitors were taken into test to gain the generation conditions of the hydrate .The results of the expriment show that the generation temperature of hydrate increase along with the raise of CO2 content .As an inhibitor ,21.8% of the ethylene glycol could lower the generation temperature about 6°C while 43.6% of it could reduce by 7-10°C .By contrast ,16.5% of methanol could lower the generation temperature about 10-16°C in the low-pressure region ,while 34.6% of it could reduced the generation temperature in all pressure region .It can be drawn that Methanol shows its better character ,but toxicity .The effects of ethylene glycol were relatively poor ,however ,little pollution .Therefore ,supposing the well-developed clearance recovery technology ,methanol was recommended .
Synthesis of Oil-Soluble Aliphatic Acylated Epicatechin and their Free Radical Scavenging Activity
Authors: Gao Zhe , Pan Yu Lei , Qin Ai Xia , Kang Xiao Hu , Li Xi Yue , Jia Ya Nan , Chen Can , Cui Tong
Published: September 2013

A series of epicatechin derivatives, including 3-O-palmitoyl epicatechin, 3-O-myristoyl epicatechin, 3-O-lauroyl epicatechin, 3-O-decanoyl epicatechin and 3-O-octanoyl epicatechin, were prepared by the acylation method. Structures of these prepared products were characterized by LC/MS and1H-NMR spectroscopy. Their antioxidant activity were assessed by the methods of DPPH and ABTS free radical scavenging. The results showed that aliphatic acyl epicatechin derivatives with varying aliphatic groups were synthesized by this method. The antioxidant activity of aliphatic acyl epicatechin derivatives were better than the oil-soluble tea polyphenol and BHT, equal to TBHQ.
Optimization for Ultra High Pressure Isomerization of Lycopene
Authors: Zong Wei , Cao Yi Heng , Jing Si Qun , Shi Hui , Zhao Guang Yuan
Published: September 2013

To isomerizate of lycopene by ultra high pressure (UHP) method, based on single factor experiments, orthogonal test was used to optimum the isomerization conditions. UHP pressure, UHP holding time, temperature were as factors and ratio of cis-lycopene in total lycopene was as index. The results were as follows: 50°C, UHP pressure 500MPa and holding time 7min. The ratio of cis-lycopene in total lycopene was 45.1%. So UHP combine heating has a isomerization effect on lycopene.
QSRR Study on GC Retention Time of Aromatic Components in Red Raspberry Wine
Authors: Cheng Li Ping , Zhang Xu Li
Published: September 2013

In the present study, quantitative structure-retention relationship (QSRR) study had been carried out for aromatic components in red raspberry wine based on various quantum-chemical and physicochemical descriptors. To build QSRR models, a multiple linear regression (MLR) stepwise method was used. The generated models have good predictive ability and are of high statistical significance with good correlation coefficients (R2≥ 0.854) and p values far less than 0.05. Preliminary results indicated that the application of the models, especially the prediction of GC retention time of aromatic components of red raspberry, will be helpful. The models contribute also to the identification of important quantum-chemical and physicochemical descriptors responsible for retention time. It was found that the shape attribute (ShpA) plays a vital role in determining component’s GC retention time. the importance of the melting point (MP) and the HOMO-LUMO energy gap (DE) of analytes is also embodied in models.
Determination of Residues of Malachite Green and Leucomalachite Green in Chinese Soft-Shelled Turtle (Pelodiscus sinensis) Muscle by Ultra-High Performance Liquid Chromatography-Mass Spectrometry
Authors: Zeng Shao Dong , Yang Chun Liang , Wang Ming Yue , Cha Yu Bing , Lin Ling
Published: September 2013

A method for the determination of residues of malachite green and leucomalachite green in Chinese soft-shelled turtles (Pelodiscus sinensis) muscle by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. The samples extracted with acetonitrile, and clean up on a neutral alumina SPE cartridge, separated on a C18 column using acetonitrile-5mmol/L ammonium acetate as mobile phase, and then detected under multiple reaction monitoring mode.The method showed a good linearity for all the analysts over the range of 0.1-50μg/L with r20.999.The detection limits of malachite green and leucomalachite green were 0.10 and 0.12μg/kg respectively. The recoveries ranged from 84.9 % to 93.8% at three spiked concentrations with the relative standard deviations lower than 5%. The results shows that this method has the advantages of easy to operate, fast to perform,with high sensitivity and accuracy, and it is suitable for detection of residues of malachite green and leucomalachite green in Chinese soft-shelled turtle (Pelodiscus sinensis).
Screening and Identification of an Indigenous Bacterial Strain High Active for Fermentative Production of White Pepper (Piper nigrum L.)
Authors: Feng Jian Cheng
Published: September 2013

An indigenous bacterial strain high active for fermentative production of white pepper was isolated from soil samples. Initially isolates were screened by yellow ring and the speed of color change in the plating medium. Then, the secondary screening was done through the abilities of the strains for the retting of green pepper. The selected strain WC17 showed excellent activity for the retting of the green pepper.The produced white pepper by this method achieved superior level. Volatile oil and piperine contents of the product were 3.26% (v/w) and 4.15% (v/w), respectively. The microbial contamination was no more than 1.3×102 per 1g. Physiological, biochemical characteristics and 16SrDNA sequencing indicated that the isolated strain WC17 was belonging to Bacillus cereus group. This screening could be used as a rapid and reliable method for the isolation of bacterial strain for fermentative production of white pepper.
Synthesis of Nanocrystalline TiO2 by NH4F-Assisted Low Temperature Hydrolysis Method and its Photocatalytic Ability for Rhodamine B
Authors: Cao Bao Wei , Peng Jian Hong , Xu Yun Hua
Published: September 2013

Nanocrystalline TiO2 powders were obtained at a low temperature between 50 and 80 °C by a hydrolysis of TiCl4 in a mixed solution of distilled water and alcohol. The as-synthesized TiO2 powders were characterized by X-ray diffraction analysis, transmission electron microscopy, and UVvisible absorption spectroscopy. Effects of the reaction temperature and the NH4F concentration on the formation of the nanocrystalline TiO2 powders were investigated. Results indicate that the reaction temperature greatly affects the particle size of the as-synthesized samples, and the NH4F concentration has a great effect on the phase structure of the as-synthesized samples. In addition, the photocatalytic activities of the as-synthesized samples for rhodamine B were also measured, and it is found that the sample synthesized from the NH4F concentration of 30 mM exhibits a higher photocatalytic activity than the P25 powder due to a high surface area and a pure anatase phase.
Application of Chitosan Composite Film to Sliced Fresh Channa argus for Shelf Life Extension
Authors: Lu Ye , Li Li , Yang Fu Xin , Zhang Heng Guang
Published: September 2013

Novel composites consisting of chitosan (Ch), polyvinyl alcohol (PVA), and thyme essential oil (T) were prepared as a new packing materials. The effects of Ch+PVA+T film on quality and shelf life of Channa argus stored under refrigeration were investigated. The control and the treated fish samples were analyzed periodically for chemical (pH, TVB-N), microbiological (total viable count), and sensory characteristics. The results indicate that samples treated remained acceptable up 15 days while the untreated samples up to11 days of storage. The thyme essential oil addition in samples contributes to a slower process of fish spoilage. The preservative effect is greater as the oil concentration is greater.
Establishment of Growth/No Growth Model for Bacillus cereus on Different Temperature, pH and Water Activity
Authors: Chen Chen , Yang Xian Shi , Li Xue Ying
Published: September 2013

The aim of this research was to establish a probabilistic model to predict the growth probability of B. cereus. The five strains of B.cereus were studied and a logistic regression model was chosen to study the interaction of different temperature (10°C, 15°C,20°C,25°C,30°C,35°C), pH(4.5, 5, 5.5, 6, 6.5, 7, 7.5), Aw( 14 levels from 0.992 to 0.932) on the probability of growth. This paper made a fractional factorial design and the experimental data was divided to two parts: 80% of data was chosen as model data and 20% of data was chosen as validation data . At last comparison was made between the predicted values and observed value by choosing 19 experimental data from the published studies. The results showed the concordance index of model data was 0.991 while the validation data was 0.976, indicating that the data was correctly classified and model had a high predictive ability. Also The performance statistics obtained indicate a reasonable goodness of fit of the model obtained, mainly due to the high values of R2-Nagelkerke (0.954) and χ2=0.0119,P=1 of the HosmerLemeshow statistic. A high predictive accuracy is obtained (89.47%) with test data, showing a good predictive ability. In this paper, the growth/no growth model can quantized the combination of environmental factors for B. cereus. This study can help food manufacturers in making decision on the more reasonable and safer formulations for food products in order to prevent the growth of B.cereus along their shelf-life.
Characteristic Wavelengths Analysis of Apple Flesh Browning Based on FT-NIR Spectroscopy
Authors: Li Gui Feng
Published: September 2013

A characteristic wavelength for measuring flesh browning of apple was put forward based on FT-NIR spectroscopy. Cluster analysis algorithm based on Euclidean distance was applied to selection of representative samples. The multivariable analysis concluding PLS and MLR were applied to build the regression models and seek the characteristic wavelengths of apple flesh browning. The loading weights from PLS model found the sensitive flesh browning wavelengths. The significance of these wavelengths was by ANOVA, and seven wavelengths (1132, 1411, 1429, 1494, 1580, 1879, and 2151nm) were selected with statistical significance at 95% confidence level. These wavelengths were strongly related with apple flesh browning by MLR models evaluated with high prediction correlation (r=0.937). These suggest the model of apple flesh browning was reliable with good predict ability and can meet the requirement to quick determination of flesh browning of apples.
Changes in Extractive Components during Spontaneous Fermentation of Fish Sauce Using Eel Intestines
Authors: Wu Run Feng , Zhao Li , Yuan Mei Lan , Su Wei , Han Jia Wang , Xu Dan Dan
Published: September 2013

To reuse the waste of fishery resources in a preliminary study, we have described changes in extractive components during spontaneous fermentation of fish sauce using eel intestines. This study was aimed at investigating the chemical analysis of extractive components from fermented eel intestines with a view to using it as a food condiment. Fish sauce was produced via fermentation of by-products (intestinal) of processed eel with 35% (w/w) of salt for 180 days. During 180 days of fermentation, the pH decreased to approximately 4.8, and the overall trend of total nitrogen, amino acid nitrogen, non-enzymatic browning index and total volatile basic nitrogen were increasing. The changes and the trend mentioned above indicated that it was a practical method to produce fish sauce using eel intestines, but the spontaneous fermentation cycle was long (more than 6 months).
An Eco-Friendly Catalytic Route for One-Pot Synthesis of Phenols from Aromatics and Hydroxylamine
Authors: Gao Li Ya , Tan Xue Jun , Xue Wei , Zhang Dong Sheng , Zhao Xin Qiang , Wang Yan Ji
Published: September 2013

A series of ionic liquids were used as a co-solvent in the reaction of one-pot synthesis of phenols from aromatics and hydroxylamine. Then an eco-friendly catalytic system, i.e., [HSO3-bmi [CF3SO3]-H2O-HAc media with (NH4)6Mo7O24·4H2O catalyst, was designed for the reaction. The results showed that aromatics were successfully hydroxylated to give the corresponding phenols. Recycling experiments suggested that the catalytic system was stable enough to be recycled.
Effects of Heat Treatment on Water Soluble Compounds of Grass Carp
Authors: Chen Qing Yun , Shi Wen Zheng , Wan Jin Qing , Wang Zhi He
Published: September 2013

Experiment was studied on water soluble compounds of grass carp (dorsal meat) with different heat treatment. The result showed that different heat treatment can cause the difference of water soluble compounds. IMP is one of degradation products of ATP, the content would increase with ATP degradation in a certain period. K value of fresh sample was 1.83%, it had no obvious difference after in the refrigerator of-20°C for 48 hours, but it increased compared with fresh sample when heated and reheated. In the dorsal meat, the contents of glycine (Gly) alanine (Ala) and histidine (His) were higher than others. The contents of total free amino acids (TFAA) contents reached maximum when reheated for fresh sample. The contents of TFAA in grass carp had no obvious regularity changes with different heat treatment.
Preliminary Analysis of Bacterial Flora in Turbot Scophthalmus maximus Cultured in Deep Well Seawater
Authors: Hou Hong Man , Zhang Gong Liang , Sun Li Ming
Published: September 2013

The microbiological characters and composition of bacterial flora in fresh turbot Scophthalmus maximus were observed with qualitative and quantitative analysis. Samples of turbot fish were collected in the spring, summer and autumn of one year. The total amounts of bacteria from the culturing water, gills and skin were quantified as 3.46±2.4-3.82±1.2 log10cfu·ml-1, 5.19±2.3-6.28±1.7 log10cfu·ml-1 and 2.70±1.1-2.95±2.8 log10cfu·ml-1, respectively. The isolated strains and bacterial flora were identified and classified. The results showed that among 206 isolated strains, Gram-positive bacteria accounted for 18.9% of the total flora, and Gram-negative bacteria formed 82.4%, including predominant bacteria such as Staphylococcusaureus (12.6%), Serratia liquefaciens (10.7%), Enterobacter (19.4%) and Pseudomonas (8.3%). A large number of isolated Enterobacter indicated that the culturing water was polluted. We report here for the first time the microbial flora in the turbot cultured in deep well seawater of Liaodong Penisula, which provides a preliminary basis for the subsequent study on the specific spoilage organisms from chilled turbot fish.
Isolation and Characteristics of Cholesterol-Lowering L. casei subsp. casei Strain GL-03 Isolated from Cheese
Authors: Hou Hong Man , Zhang Gong Liang , Sun Li Ming
Published: September 2013

Cholesterol-lowering effect of lactic acid bacteria is well-known. In this study, nine cholesterol-lowering Lactobacillus strains from Chinese traditional cheese, pickle and yoghurt were screened and characterized for their potential use. The microbial content of all the strains was significantly decreased at pH 1.5, but the residual counts of L. casei subsp. casei GL-03, L. plantarum ZP-Z, L. plantarum ZP-05 and L. brevis ZP-04 were more than 107cfu/mL after 6h of incubation. All the nine strains of Lactobacillus indicated good tolerance to bile at concentration less than 0.2% after 2 or 6 h of incubation. L. plantarum ZP-W had maximum hydrophobicity towards xylene while GL-03 strain possessed maximum hydrophobicity for both hexadecane and octane. ZP-05 strain had more effective inhibitory activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Shigella dysenteriae than other eight strains. These results suggest that L. casei subsp. casei GL-03 may be effective as a probiotic with cholesterol-lowing activities.
Characterization of Tomato Fruit LeRIN Gene Application Virus-Induced Gene Silencing Technology
Authors: Li Ling , Zhu Guo Xia , Liu Zheng , Liu Tie Ling
Published: September 2013

Virus-induced gene silencing (VIGS) is a powerful tool for the study of gene function and down regulate specific gene expression in plant. The RIN transcription factor is an important developmental regulator in tomato fruit ripening. In this work, the TRV-LeRIN infiltrated fruit significantly developed green color phenotypes, whereas the control fruit would turn red normally. Dissection of silenced areas for analysis identified whether the LeRIN gene is silenced or not. Taken together, we successfully applied syringe infiltration method of VIGS to silence the LeRIN gene in tomato fruit. These results are critical for understanding the mechanism of tomato fruit ripening.
Research on the Antioxidation of Polyrhachis vicina Wine
Authors: Sun Jing Xian , Yang Hai Ying , Zhou Ping , Zhang Guang Qiu , Du Gang , Gao Yun Tao
Published: September 2013

The antioxidant activity of Polyrhachis vicina wine extract was investigated: The IC50 of scavenging effect on superoxide radical generated by Pyrogallol autoxidation was 0.37 mg/mL; the maximum scavenging rate was 81.4%. The IC50 of scavenging effect on hydroxyl free radical generated by Fenton reaction was 0.66 mg/mL; the maximum scavenging rate was 91.8%. The IC50 of DPPH· scavenging effect was 0.48 mg/mL; the maximum scavenging rate was 91.4%. The results indicated that Polyrhachis vicina wine extract showed activity both in scavenging free radicals and antioxidation.
Optimization of the Combination of Three Natural Preservatives in Beef Storage with Controlled Freezing Point Technique
Authors: Sun Tian Li , Zhang Ping , Ren Zhao Hui , Zhang Peng , Yue Xi Qing
Published: September 2013

Response surface methodology (RSM), based on Box-Behnken design principle, was employed to obtain the optimum combination of three natural preservatives in beef storage with controlled freezing point technique. Chitosan, Nisin and tea polyphenols were chosen as the optimization factors while investigated responses was total volatile basic nitrogen (TVB-N). The response surface plot and the contour plot derived from the mathematical models were applied to determine the optimal conditions. The Optimum combination of three preservatives was chitosan 1.70%, Nisin 0.03% and tea polyphenols 0.24%. The increasing rate of TVB-N was 49.34% on the 20thday when beef was stored in controlled freezing point condition with the optimum combination of three preservatives, which was in good agreement with the value predicted by the model.
Effect of Coconut Water on the Growth of Gluconacetobacter xylinus Y15
Authors: Li Lin Jin , Liu Si Xin , Li Cong Fa
Published: September 2013

The effect of coconut water contents, sucrose and ammonium sulfate concentrations, cultivation modes on the growth of Gluconacetobacter xylinus Y15 was investigated. When 25%(V/V) coconut water, 3g/L (NH4)2SO4 was added into the medium without sucrose, and G. xylinus Y15 was cultivated in shake flask culture at 130r/min for 24h, the cell number could reach as high as 4.9×108CFU/mL, while the cell number was only 4.9×104CFU/mL when cultivated in static culture. The growth curves of G. xylinus Y15 in coconut water medium and HS [ medium in shake flask culture were compared. The lag phase of G. xylinus Y15 in coconut water medium was 20h shorter than in HS medium, and the log phase was 20h longer.
Performance of Indirect ELISA for Maize Hybrid Purity Test
Authors: Liu Yue Hui , Wang Jun , Yang Xin Chao , Sun Na Xin , Xu Li Hua
Published: September 2013

Maize is the second large crop in China that needed 100-120 million tons seeds for an average year and the production loss caused by inferior seeds was amazing. Bing an allogamous wind-pollinated plant, maize hybrid purity problem is mainly reflected the inbred lines seed mixed in hybrid. Therefore it is important to have specific and sensitive purity test methods to prevent the poor seeds into market. In this paper, we developed an inderect ELISA using polyconal antibody against maize inbred lines to bind extracts from whole grain with pure water. The single blind trial demonstrated that the average detectable rate of indrect ELISA was 95.2%. We also extended this method to other maize hybrid varieties, found it has no ability to descriminate the maize hybrid facticity.
Optimization of Extraction Process for Carotenoids from Tamarillo Fruits by Response Surface Methodology
Authors: Yang Qing Song , Zhao Yan
Published: September 2013

Using Box-Behnken center composite design of three variables with three levels, the optimal extraction process for carotenoids from tamarillo fruits were determined by response surface methodology. The optimal extraction process was obtained as followed: solid-liquid ratio of 1:19.02(g/mL), extraction temperature of 44°C, and extraction time of 33.68 min. Under these conditions, the extraction of carotenoids content was 5.755[mg/100, similar to the theoretical prediction of 5.885[mg/100. This study confirms the efficiency of RSM as a simple and effective method and the great potential of tamarillo fruits as a health food and a resource of carotenoids.
Total Flavonoids Content was Improved by Chalcone Synthase Overexpression in Callus Cell of Fagopyrum tataricum
Authors: Liu Kai , Wang Guan , Yu Han Song , Hu Yao Hui
Published: September 2013

According to the online software, the primer is designed by CODEHOPE(Consensus De-generate Hybrid Oligonucleotide Primers),then the cDNA sequence of Chalcone synthase was obtained by the method of RACE(GenBank No. HQ434624).Then the high efficiency vector named pCM-BCHS was constructed by pCAMBIA1301 and Chalcone synthase gene. The vector was transformed byAgrobacterium tumefaciensto buckwheat induced callus cell. At last the selected transgene callus cell can synthase the total flavonoid at 48.3mg/g DW, which is two times of the wild type. Therefore, it is proved that the overexpression of Chalcone synthase will enhance the metablic flux of total flavonoid synthesis.
Characteristics of Soybean Meals by Solid State Fermentation Using Bacillus subtilis GA15
Authors: Yang Xiao Jia , Wang Jin Shui , Yang Sen
Published: September 2013

Characteristics of solid state fermentation for soybean meals were studied in present paper. Effects of different factors on protease activity and peptides content during fermentation, using Bacillus Subtilis GA15 were explored. Optimal conditions of moisture content 50%, substrate height 2.5 cm at 36 °C for 72 h of incubation were obtained. Peptide content could be up to 14.37% in this condition. Moreover, the allergens in soybean meals were almost eliminated during fermentation according to SDS-PAGE profile.
Effects of Hanseniaspora uvarum Integrated with Salicylic Acid or Sodium Bicarbonate on Postharvest Decay of Grapes
Authors: Qin Xiao Jie , Xiao Hong Mei , Liu Li , Gao Jin Xiang , Wang Xiao Xia , Yang Rong
Published: September 2013

This study was conducted to investigate the application of the antagonistic yeast strain ofHanseniaspora uvarumcombined with salicylic acid (SA) and sodium bicarbonate (SBC) to create an efficient integrated approach to control grey mould on grapes. The results indicated that the treatment of 1×108CFU/mlH. uvarumcombined with SA at 2 mM and combined with SBC at 2% resulted in a remarkably improved control ofB. cinereainfections on grapes at 25°C,RH 95%. In experimental trials, the average lesion diameter of the samples treated withH.uvarumplus 2 mM SA andH. uvarumplus 2% SBC after 3 days were both 0.81 cm, which were significantly smaller compared with that of the samples treated withH. uvarumindividually (0.891 cm; 0.9 cm) and with sterile distilled water (1.318 cm; 1.36 cm). Furthermore, integration ofH. uvarumwith SA and with SBC both significantly reduced the decay rate, leave rate, weight loss while maintained the firmness, titratable acidity (TA), total soluble solids (TSS) of the grapes. The proper combination of antagonist yeasts and chemical reagent can thus provide an effective strategy to reduce postharvest decay of grape fruit.