bluuebunny/crossref_metadata_2025 huggingface dataset:
Reverse-Phase Protein Array for Prediction of Patients at Low Risk of Developing Bone Metastasis From Breast Cancer
Authors: Hayashi Naoki , Manyam Ganiraju C. , Gonzalez-Angulo Ana M. , Niikura Naoki , Yamauchi Hideko , Nakamura Seigo , Hortobágyi Gabriel N. , Baggerly Keith A. , Ueno Naoto T.
Published: August 2014

Background.

A biomarker that predicts bone metastasis based on a protein laboratory assay has not been demonstrated. Reverse-phase protein array (RPPA) enables quantification of total and phosphorylated proteins, providing information about their functional status. The aim of this study was to identify bone-metastasis-related markers in patients with primary breast cancer using RPPA analysis.

Patients and Methods.

Tumor samples were obtained from 169 patients with primary invasive breast carcinoma who underwent surgery. The patients were categorized by whether they developed breast cancer bone metastasis (BCBM) during follow-up. Clinical characteristics and protein expression by RPPA were compared and verified by leave-one-out cross-validation.

Results.

Lymph node status (p = .023) and expression level of 22 proteins by RPPA were significantly correlated with BCBM in logistic regression analysis. These variables were used to build a logistic regression model. After filtering the variables through a stepwise algorithm, the final model, consisting of 8 proteins and lymph node status, had sensitivity of 30.0%, specificity of 90.5%, positive predictive value of 30.0%, and negative predictive value of 90.5% in the cross-validation. Most of the identified proteins were associated with cell cycle or signal transduction (CDK2, CDKN1A, Rb1, Src, phosphorylated-ribosomal S6 kinase, HER2, BCL11A, and MYH11).

Conclusion.

Our validated model, in which the primary tumor is tested with RPPA, can predict patients who are at low risk of developing BCBM and thus who likely would not benefit from receiving a bisphosphonate in the adjuvant setting. Clinical trials excluding these patients have the potential to clarify the benefit of bisphosphonates in the adjuvant setting.
Database of wide concrete members failing in shear
Authors: Lantsoght Eva O. L. , van der Veen Cor , Walraven Joost C. , de Boer Ane
Published: August 2014

Shear in reinforced members has been a topic of study for many decades. Recently, the shear capacity of slabs subjected to concentrated loads – the case between one-way shear (also called beam shear) and two-way shear (or punching shear) – has been given more attention because this case is encountered in bridge engineering. This paper aims to give an overview of the existing code methods for shear and to bring together experiments from the literature on wide beams and slabs failing in shear. The database of collected experiments is then compared with the Eurocode provisions. A large scatter was found in the ratio of experimental to predicted values. This observation indicates that the experiments under consideration should be studied in subsets according to the failure mode and that better methods for determining the shear capacity of wide concrete members are necessary. The database also shows the need for experiments aimed at studying shear in one-way slabs and the effect of different parameters on the shear capacity.
Ecological differentiation and local adaptation in two sister species of Neotropical Costus (Costaceae)
Authors: Chen Grace F. , Schemske Douglas W.
Published: August 2014

Reciprocal transplant experiments have often provided evidence of local adaptation in temperate plants, but few such studies have been conducted in the tropics. To enhance our knowledge of local adaptation in tropical plants, we studied natural populations of two recently diverged Neotropical plant species, Costus allenii and C. villosissimus, in central Panama. We found that these species display a parapatric distribution that reflects local environmental differences on a fine geographic scale: C. allenii is found along ravines in the understory of primary forest, while C. villosissimus is found along forest edges. Light availability was lower in C. allenii habitats, while precipitation and soil moisture were lower in C. villosissimus habitats. We carried out reciprocal transplant experiments with seeds and clones of mature plants to test the hypothesis that the parapatric distribution of these species is due to divergent adaptation to their local habitats. We found strong evidence of local adaptation, i.e., when grown in their “home” sites, each species outperformed the species from an “away” site. Our finding that C. allenii and C. villosissimus are mainly isolated by their microhabitats provides a first step toward understanding the mechanisms of adaptation and speciation in the tropics.
Modeling of Anaerobic Digestion with a Focus on Estimation of Hydrolysis Constants at 35, 55, and 60 °C
Authors: Haghighatafshar Salar , Ossiansson Elin , Koch Konrad , Kjerstadius Hamse , Jansen Jes la Cour , Davidsson Åsa
Published: August 2014

Hydrolysis constants of mixed sludge at 35, 55, and 60 °C were found to be 0.32, 0.44, and 0.50 1/d, respectively, in pilot‐scale, semicontinuously operated anaerobic digesters. The hydrolysis constants and estimated chemical oxygen demand fractions in the feed were introduced to a mathematical model for anaerobic digestion published by Siegrist et al. (2002), which is similar to Anaerobic Digestion Model No. 1. First‐order and Monod‐type kinetics were tested for estimation of hydrolysis constants. The applied kinetics were found to affect the outcome of the regression study. Moreover, the free ammonia inhibition model was excluded for both propionate oxidation and acetate conversion, thanks to the apparent acclimatized biomass. No substantial accumulation of volatile fatty acids was observed in the reactors at 35, 55, and 60 °C, corresponding to free ammonia nitrogen concentrations of about 20, 110, and 130 g N/m3, respectively.
Nursing Home Residents and Celebrities: A Tale of Morality
Authors: Claessens Nathalie
Published: August 2014

In contemporary Western societies, characterized by global aging and an omnipresent celebrity culture, little is known about the role of celebrities for older adults. This study bridges gerontology and celebrity studies to explore a social role that celebrities can fulfill for nursing home residents: triggering moral discussions. This potential role is examined in four focus groups with 27 nursing home residents in Flanders (Belgium). Here, 20 celebrity pictures are employed to evoke moral discussions, with a focus on adultery and homosexuality. These discussions are subjected to a framing analysis. Results show that celebrities can trigger moral discussions among the nursing home residents. The residents' adultery and homosexuality frames show that they mostly retain dominant values from their youth, often combining them with contemporary dominant values. Further, the residents' frames prove to be relativistic, which can be linked to their multitude of life experiences and complex emotional skills.
Overcoming Obstacles and Academic Hope: An Examination of Factors Promoting Effective Academic Success Strategies
Authors: Hansen Michele Joann , Trujillo Daniel J. , Boland Donna L. , MaCkinnon Joyce L.
Published: August 2014

The purpose of this qualitative study was to explore the underlying non-cognitive processes and institutional factors that allowed first-year students to enact effective strategies for attaining academic success and persisting despite obstacles. The varying levels of academic preparation and unique obstacles faced by the student participants allowed for us to employ a constant comparative approach in analyzing the results. A core story of hopeful thinking emerged which interacted with four major themes including: a) generating alternative pathways when encountering obstacles to social and academic integration, b) goal setting and planning, c) active use of peer and academic supports, and d) experiencing a positive institutional context. While this study confirms research about what factors predict the success of students coping with difficult transitions, it also provides new insights. Results suggest that academic hope is an important theoretical framework to guide future research and practice in the area of student retention.
Institutional Characteristics and Student Retention in Public 4-Year Colleges and Universities
Authors: Marsh Gregory
Published: August 2014

The purpose of this study was to determine the relative importance of specific student and institutional factors and characteristics on the retention of students at public, 4-year colleges and universities in the United States based on aggregate student cohort and institutional data. The overriding goal of this research was to identify any institutional characteristics that may improve student retention rates, which would provide administrators with valuable information for planning purposes. Hierarchical multiple regression was utilized to measure the relative effect of each of five blocks of independent variables on institutional student retention rates. Results of the study indicated that while entering student characteristics were most successful in explaining variances in retention rates among institutions, the various institutional characteristic blocks also had significant effects on student retention rates, to varying degrees. Results from this study may be instructive for college and university administrators in the institutional decision-making process.
The Impacts of Virtual Manipulatives and Prior Knowledge on Geometry Learning Performance in Junior High School
Authors: Lee Chun-Yi , Chen Ming-Jang
Published: August 2014

Previous studies on the effects of virtual and physical manipulatives have failed to consider the impact of prior knowledge on the efficacy of manipulatives. This study focuses on the learning of plane geometry in junior high schools, including the sum of interior angles in polygons, the sum of exterior angles in polygons, and the properties of parallel lines. This study adopted a quasi-experimental design of pre-test and post-tests with nonequivalent groups. The participants comprised four classes in the 8th grade. Students were randomly divided into two groups: the virtual manipulatives group and the physical manipulatives group. Two-way factorial analysis of covariance was adopted to compare the impact of manipulatives (virtual vs. physical) and prior knowledge (high vs. low) on learning outcomes and attitudes toward mathematics. Our results demonstrated that students with high prior knowledge using virtual manipulatives had better posttest performance than did the physical manipulatives group, and reported taking greater enjoyment in mathematics. These students also perceived the importance of mathematics more strongly than those with low prior knowledge. Students with high prior knowledge also presented stronger motivation to study mathematics and freedom from fear of the subject than those with low prior knowledge.
Making Student Thinking Visible through a Concept Map in Computer-Based Assessment of Critical Thinking
Authors: Rosen Yigal , Tager Maryam
Published: August 2014

Major educational initiatives in the world place great emphasis on fostering rich computer-based environments of assessment that make student thinking and reasoning visible. Using thinking tools engages students in a variety of critical and complex thinking, such as evaluating, analyzing, and decision making. The aim of this study was to explore patterns in student critical thinking performance and motivation in Evidence-Centered Concept Map (ECCM) mode, compared to basic notepad mode. One hundred ninety 14-year-old students from the United States, United Kingdom, Singapore, and South Africa participated in the study. Students in both modes were able to analyze a multifaceted dilemma by using similar information resources. In the ECCM mode, students used ECCM to organize their thinking; in another mode, students were provided with a basic online notepad to make records as needed. Overall, the findings showed that students assessed in ECCM mode outperformed their peers in notepad mode in critical thinking skills. Students who worked with ECCM provided more informed recommendations by using supporting evidence from the available resources and discussing alternative points of view on the topic. The findings showed a significantly positive relationship between student recommendation score and the ability to develop ECCM. In addition, the results demonstrated that it did not matter for students' motivation whether they analyzed the dilemma with or without ECCM. Directions for future research are discussed in terms of their implications for large-scale assessment programs, teaching, and learning.
Complete Non-Orientable Minimal Surfaces in ℝ3 and Asymptotic Behavior
Authors: Alarcón Antonio , López Francisco J.
Published: August 2014

In this paperwe give new existence results for complete non-orientable minimal surfaces in ℝ3 with prescribed topology and asymptotic behavior
Optimizing Firm Inventory Costs as a Fuzzy Problem
Authors: Kacprzak Dariusz , Kosiński Witold
Published: August 2014

The fixed order quantity model of inventory management system is used in the deterministic part. Several elements of inventory cost, such as ordering cost, transportation and storing costs, frozen capital cost, as well as extra rebates, are taken into account in the model. Then the fuzzy optimization problem for the total cost function is formulated within the space of Ordered Fuzzy Numbers when all variables of the model are fuzzy. After the choice of a particular defuzzification functional an appropriate theorem is formulated which gives the solution of the problem.
Clustering Algorithms in Hybrid Recommender System on MovieLens Data
Authors: Kuzelewska Urszula
Published: August 2014

Decisions are taken by humans very often during professional as well as leisure activities. It is particularly evident during surfing the Internet: selecting web sites to explore, choosing needed information in search engine results or deciding which product to buy in an on-line store. Recommender systems are electronic applications, the aim of which is to support humans in this decision making process. They are widely used in many applications: adaptive WWW servers, e-learning, music and video preferences, internet stores etc. In on-line solutions, such as e-shops or libraries, the aim of recommendations is to show customers the products which they are probably interested in. As input data the following are taken: shopping basket archives, ratings of the products or servers log files.

The article presents a solution of recommender system which helps users to select an interesting product. The system analyses data from other customers' ratings of the products. It uses clustering methods to find similarities among the users and proposed techniques to identify users' profiles. The system was implemented in Apache Mahout environment and tested on a movie database. Selected similarity measures are based on: Euclidean distance, cosine as well as correlation coefficient and loglikehood function.
Number of Clusters and the Quality of Hybrid Predictive Models in Analytical CRM
Authors: Łapczyński Mariusz , Jefmański Bartłomiej
Published: August 2014

Making more accurate marketing decisions by managers requires building effective predictive models. Typically, these models specify the probability of customer belonging to a particular category, group or segment. The analytical CRM categories refer to customers interested in starting cooperation with the company (acquisition models), customers who purchase additional products (cross- and up-sell models) or customers intending to resign from the cooperation (churn models). During building predictive models researchers use analytical tools from various disciplines with an emphasis on their best performance. This article attempts to build a hybrid predictive model combining decision trees (C&RT algorithm) and cluster analysis (k-means). During experiments five different cluster validity indices and eight datasets were used. The performance of models was evaluated by using popular measures such as: accuracy, precision, recall, G-mean, F-measure and lift in the first and in the second decile. The authors tried to find a connection between the number of clusters and models' quality.
The Multi-Criteria Negotiation Analysis Based on the Membership Function
Authors: Roszkowska Ewa , Wachowicz Tomasz
Published: August 2014

In this paper we propose a multi-criteria model based on the fuzzy preferences approach which can be implemented in the prenegotiation phase to evaluate the negotiations packages. The applicability of some multi-criteria ranking methods were discussed for building a scoring function for negotiation packages. The first one is Simple Additive Weighting (SAW) technique which determines the sum of the partial satisfactions from each negotiation issue and aggregate them using the issue weights. The other one is Distance Based Methods (DBM), with its extension based on the distances to ideal or anti-ideal package, i.e. the TOPSIS procedure. In our approach the negotiator's preferences over the issues are represented by fuzzy membership functions and next a selected multi-criteria decision making method is adopted to determine the global rating of each package. The membership functions are used here as the equivalents of utility functions spread over the negotiation issues, which let us compare different type of data. One of the key advantages of the approach proposed is its usefulness for building a general scoring function in the ill-structured negotiation problem, namely the situation in which the problem itself as well as the negotiators preferences cannot be precisely defined, the available information is uncertain, subjective and vague. Secondly, all proposed variants of scoring functions produce consistent rankings, even though the new packages are added (or removed) and do not result in rank reversal.
Equitable Distribution in a Three Players Problem
Authors: Szopa Marek
Published: August 2014

Jazz band is a 3 player superadditive game in characteristic function form. Three players have to divide the payoff they can get, while being in a grand coalition, provided their individual and duo coalitions payoffs are known. Assumptions of individual and collective rationality lead to the notion of the core of the game. We discuss offers that cannot readily be refused [OCRR] as the solutions of the game in case of an empty core, when duo coalitions are the best options but only for two out of three players. The experiment shows that even in case of an empty core the most probable results are three-way coalitions and the share of the weakest player usually exceeds his OCRR. The Shapley value is introduced and its fairness is discussed as it lies at the side of the core while, on the other hand, the nucleolus lies exactly at the center of the core. We conclude that, in spite of that, the Shapley value is the best candidate for a fair sharing solution of the jazz band game and other similar games as, opposite to the other values, it is dependent both on individual and duo coalitions payoffs.
Prognosis of Postviral Olfactory Loss: Follow-up Study for Longer than One Year
Authors: Lee Doh Young , Lee Woo Hyun , Wee Jee Hye , Kim Jeong-Whun
Published: August 2014

Background

There have been very few studies on the prognosis of postviral olfactory dysfunction. The aim of the study was to evaluate its long-term prognosis and factors influencing the prognosis.

Methods

This study included patients who were diagnosed with postviral olfactory dysfunction at least one year ago in a Smell and Taste Clinic and whose telephone interviews were available. Their medical records were reviewed, and they were asked to score their subjective olfactory function on a 100-point scale by telephone interview.

Results

A total of 63 patients and 20 normal controls without nasal pathology were included. The mean of the patients was 49.5 years, and 19 males and 44 females were included. The mean length of follow-up was 33.4 months. Olfactory dysfunction was subjectively improved in 85.7% of the patients. The recovery rate to subjective normosmia was 31.7%. The mean symptom scores for smell significantly increased in comparison with baseline from 25.6 to 69.0. Among the 25 patients (37.7%) who underwent follow-up butanol threshold test (BTT), the score improved from 4.6 to 7.3. Olfactory improvement was more frequently reported by females (p = .003) and in patients with follow-up duration for longer than 2 years (p = .043).

Conclusions

Follow-up of postviral olfactory loss revealed that over 80% of the patients reported subjective recovery after one year. The more favorable prognosis was associated with longer follow-up duration and female gender.
Self-Cleaning Photocatalytic Polyurethane Coatings Containing Modified C60 Fullerene Additives
Authors: Lundin Jeffrey , Giles Spencer , Cozzens Robert , Wynne James
Published: August 2014

Surfaces are often coated with paint for improved aesthetics and protection; however, additional functionalities that impart continuous self-decontaminating and self-cleaning properties would be extremely advantageous. In this report, photochemical additives based on C60 fullerene were incorporated into polyurethane coatings to investigate their coating compatibility and ability to impart chemical decontaminating capability to the coating surface. C60 exhibits unique photophysical properties, including the capability to generate singlet oxygen upon exposure to visible light; however, C60 fullerene exhibits poor solubility in solvents commonly employed in coating applications. A modified C60 containing a hydrophilic moiety was synthesized to improve polyurethane compatibility and facilitate segregation to the polymer–air interface. Bulk properties of the polyurethane films were analyzed to investigate additive–coating compatibility. Coatings containing photoactive additives were subjected to self-decontamination challenges against representative chemical contaminants and the effects of additive loading concentration, light exposure, and time on chemical decontamination are reported. Covalent attachment of an ethylene glycol tail to C60 improved its solubility and dispersion in a hydrophobic polyurethane matrix. Decomposition products resulting from oxidation were observed in addition to a direct correlation between additive loading concentration and decomposition of surface-residing contaminants. The degradation pathways deduced from contaminant challenge byproduct analyses are detailed.
Dialectic Form Finding of Passive and Adaptive Shading Enclosures
Authors: Adriaenssens Sigrid , Rhode-Barbarigos Landolf , Kilian Axel , Baverel Olivier , Charpentier Victor , Horner Matthew , Buzatu Denisa
Published: August 2014

Form finding describes the process of finding a stable equilibrium shape for a system under a specific set of loads, for a set of boundary conditions and starting from an arbitrary initial geometry. However, form finding does not traditionally involve performance constraints such as energy-related criteria. Dialectic form finding is an extension of the process integrating energy-related design aspects. In this paper, dialectic form finding is employed as an approach for designing high performance architectural systems, driven by solar radiation control and structural efficiency. Two applications of dialectic form found shading enclosure structures, a passive and an active one, are presented. The first application example is a site-specific outdoor shading structure. The structure is based on a louver system designed to provide protection from ultraviolet radiation over a pre-defined target only when required, promoting natural lighting and ventilation. The second application example is a shape-shifting modular façade system that adapts its opacity in response to environmental fluctuations. The system can thus improve the environmental performance of a building. Moreover, the system explores elastic deformations for shape changes, reducing actuation requirements. These examples highlight the potential of the dialectic form-finding strategy for the design of high performance architectural integrated structures.
Should I Care about Your Opinion? Detection of Opinion Interestingness and Dynamics in Social Media
Authors: Maynard Diana , Gossen Gerhard , Funk Adam , Fisichella Marco
Published: August 2014

In this paper, we describe a set of reusable text processing components for extracting opinionated information from social media, rating it for interestingness, and for detecting opinion events. We have developed applications in GATE to extract named entities, terms and events and to detect opinions about them, which are then used as the starting point for opinion event detection. The opinions are then aggregated over larger sections of text, to give some overall sentiment about topics and documents, and also some degree of information about interestingness based on opinion diversity. We go beyond traditional opinion mining techniques in a number of ways: by focusing on specific opinion-target extraction related to key terms and events, by examining and dealing with a number of specific linguistic phenomena, by analysing and visualising opinion dynamics over time, and by aggregating the opinions in different ways for a more flexible view of the information contained in the documents.
Health Effects Associated with Inhalation of Airborne Arsenic Arising from Mining Operations
Authors: Martin Rachael , Dowling Kim , Pearce Dora , Sillitoe James , Florentine Singarayer
Published: August 2014

Arsenic in dust and aerosol generated by mining, mineral processing and metallurgical extraction industries, is a serious threat to human populations throughout the world. Major sources of contamination include smelting operations, coal combustion, hard rock mining, as well as their associated waste products, including fly ash, mine wastes and tailings. The number of uncontained arsenic-rich mine waste sites throughout the world is of growing concern, as is the number of people at risk of exposure. Inhalation exposures to arsenic-bearing dusts and aerosol, in both occupational and environmental settings, have been definitively linked to increased systemic uptake, as well as carcinogenic and non-carcinogenic health outcomes. It is therefore becoming increasingly important to identify human populations and sensitive sub-populations at risk of exposure, and to better understand the modes of action for pulmonary arsenic toxicity and carcinogenesis. In this paper we explore the contribution of smelting, coal combustion, hard rock mining and their associated waste products to atmospheric arsenic. We also report on the current understanding of the health effects of inhaled arsenic, citing results from various toxicological, biomedical and epidemiological studies. This review is particularly aimed at those researchers engaged in the distinct, but complementary areas of arsenic research within the multidisciplinary field of medical geology.
Melatonin Suppresses Hypoxia-Induced Migration of HUVECs via Inhibition of ERK/Rac1 Activation
Authors: Yang Ling , Zheng Jianchao , Xu Rui , Zhang Yujie , Gu Luo , Dong Jing , Zhu Yichao , Zhou Ruijue , Zheng Lu , Zhang Xiaoying , Du Jun
Published: August 2014

Melatonin, a naturally-occurring hormone, possesses antioxidant properties and ameliorates vascular endothelial dysfunction. In this study, we evaluate the impact of melatonin on the migratory capability of human umbilical vein endothelial cells (HUVECs) to hypoxia and further investigate whether ERK/Rac1 signaling is involved in this process. Here, we found that melatonin inhibited hypoxia-stimulated hypoxia-inducible factor-1α (HIF-1α) expression and cell migration in a dose-dependent manner. Mechanistically, melatonin inhibited Rac1 activation and suppressed the co-localized Rac1 and F-actin on the membrane of HUVECs under hypoxic condition. In addition, the blockade of Rac1 activation with ectopic expression of an inactive mutant form of Rac1-T17N suppressed HIF-1α expression and cell migration in response to hypoxia, as well, but constitutive activation of Rac1 mutant Rac1-V12 restored HIF-1α expression, preventing the inhibition of melatonin on cell migration. Furthermore, the anti-Rac1 effect of melatonin in HUVECs appeared to be associated with its inhibition of ERK phosphorylation, but not that of the PI3k/Akt signaling pathway. Taken together, our work indicates that melatonin exerts an anti-migratory effect on hypoxic HUVECs by blocking ERK/Rac1 activation and subsequent HIF-1α upregulation.
Consequences of Abdominal Adiposity within the Metabolic Syndrome Paradigm in Black People of African Ancestry
Authors: Gaillard Trudy
Published: August 2014

The metabolic syndrome (MetS) is a constellation of risk factors that are associated with increased risks for coronary heart disease and type 2 diabetes. Although the cause is unknown, abdominal adiposity is considered the underpinning of these metabolic alterations. Hence, increased abdominal adiposity contributes to dyslipidemia, hyperglycemia, beta cell dysfunction, insulin resistance, hypertension and inflammation. The role of abdominal adiposity in the causation of metabolic alterations that lead to the clinical expression of the MetS has become a focus of active research. In addition, there are ethnic/racial differences in the manifestation of the MetS. Therefore, the focus of this current review is to: (1) explore the consequences of abdominal obesity within the MetS paradigm; and (2) discuss the impact of ethnicity/race on MetS in Black People of African Ancestry (PAA).
Epigalloccatechin-3-gallate Inhibits Ocular Neovascularization and Vascular Permeability in Human Retinal Pigment Epithelial and Human Retinal Microvascular Endothelial Cells via Suppression of MMP-9 and VEGF Activation
Authors: Lee Hak , Jun Jae-Hyun , Jung Eun-Ha , Koo Bon , Kim Yeong
Published: August 2014

Epigalloccatechin-3-gallate (EGCG) is the main polyphenol component of green tea (leaves of Camellia sinensis). EGCG is known for its antioxidant, anti-inflammatory, antiviral, and anti-carcinogenic properties. Here, we identify EGCG as a new inhibitor of ocular angiogenesis and its vascular permeability. Matrix metalloproteinases (MMPs) and vascular endothelial growth factor (VEGF) play a key role in the processes of extracellular matrix (ECM) remodeling and microvascular permeability during angiogenesis. We investigated the inhibitory effects of EGCG on ocular neovascularization and vascular permeability using the retina oriented cells and animal models induced by VEGF and alkaline burn. EGCG treatment significantly decreased mRNA and protein expression levels of MMP-9 in the presence of 12-O-tetradecanoylphorbol-13-acetate (TPA) and tumor necrosis factor alpha (TNF-α) in human retinal pigment epithelial cells (HRPECs). EGCG also effectively protected ARPE-19 cells from cell death and attenuated mRNA expressions of key angiogenic factors (MMP-9, VEGF, VEGF Receptor-2) by inhibiting generation of reactive oxygen species (ROS). EGCG significantly inhibited proliferation, vascular permeability, and tube formation in VEGF-induced human retinal microvascular endothelial cells (HRMECs). Furthermore, EGCG significantly reduced vascular leakage and permeability by blood-retinal barrier breakdown in VEGF-induced animal models. In addition, EGCG effectively limited upregulation of MMP-9 and platelet endothelial cell adhesion molecule (PECAM/CD31) on corneal neovascularization (CNV) induced by alkaline burn. Our data suggest that MMP-9 and VEGF are key therapeutic targets of EGCG for treatment and prevention of ocular angiogenic diseases such as age-related macular degeneration, diabetic retinopathy, and corneal neovascularization.
Tryptophan as a Probe to Study the Anticancer Mechanism of Action and Specificity of α-Helical Anticancer Peptides
Authors: Li Guirong , Huang Yibing , Feng Qi , Chen Yuxin
Published: August 2014

In the present study, a single tryptophan, as a fluorescence probe, was shifted from the N-terminus to the middle and to the C-terminus of a 26-residue α-helical anticancer peptide sequence to study the mechanism of action and specificity. The hydrophobicity of peptides, as well as peptide helicity and self-associating ability, were slightly influenced by the position change of tryptophan in the peptide sequence, while the hemolytic activity and anticancer activity of the peptide analogs remained the same. The tryptophan fluorescence experiment demonstrated that peptide analogs were more selective against LUVs mimicking cancer cell membranes than LUVs mimicking normal cell membranes. During the interaction with target membranes, the N-terminus of an anticancer peptide may be inserted vertically or tilted into the hydrophobic components of the phospholipid bilayer first. The thermodynamic parameters of the peptides PNW and PCW, when interacting with zwitterionic DMPC or negatively charged DMPS, were determined by ITC. DSC experiments showed that peptide analogs significantly altered the phase transition profiles of DMPC, but did not dramatically modify the phase transition of DMPS. It is demonstrated that hydrophobic interactions are the main driving force for peptides interacting with normal cell membranes, whilst, electrostatic interactions dominate the interactions between peptides and cancer cell membranes. Utilizing tryptophan as a fluorescence probe molecule appears to be a practicable approach to determine the interaction of peptides with phospholipid bilayers.
The Toxicity Mechanisms of Action of Aβ25–35 in Isolated Rat Cardiac Myocytes
Authors: Zhang Beiru , Bian Xiaohui , He Ping , Fu Xiaoying , Higuchi Keiichi , Yang Xu , Li Detian
Published: August 2014

β-Amyloid (Aβ) is deposited in neurons and vascular cells of the brain and is characterized as a pathologic feature of Alzheimer’s disease (AD). Recently studies have reported that there is an association between cardiovascular risk factors and AD, however the mechanism of this association is still uncertain. In this study we observed Aβ had an effect on cardiovascular cells. We represent as a major discovery that Aβ25–35 had toxicity on isolated rat cardiac myocytes by impacting the cytoskeleton assembly and causing ER stress, ultimately contributing to the apoptosis of the myocytes. Importantly, the activation of ER stress and subsequent cellular dysfunction and apoptosis by Aβ25–35 was regulated by the MAPK pathway, which could be prevented by inhibition of p38 via pharmacological inhibitors. It was noteworthy that Aβ25–35 played a critical role in cardiac myocytes, suggesting that Alzheimer’s disease (AD) had a relation with the heart and understanding of these associations in future will help search for effective treatment strategies.
Non-Stoichiometric Polymer-Cyclodextrin Inclusion Compounds: Constraints Placed on Un-Included Chain Portions Tethered at Both Ends and Their Relation to Polymer Brushes
Authors: Tonelli Alan
Published: August 2014

When non-covalently bonded crystalline inclusion compounds (ICs) are formed by threading the host cyclic starches, cyclodextrins (CDs), onto guest polymer chains, and excess polymer is employed, non-stoichiometric (n-s)-polymer-CD-ICs, with partially uncovered and “dangling” chains result. The crystalline host CD lattice is stable to ~300 °C, and the uncovered, yet constrained, portions of the guest chains emanating from the CD-IC crystal surfaces behave very distinctly from their neat bulk samples. In CD-IC crystals formed with α- and γ-CD hosts, each containing, respectively, six and eight 1,4-α-linked glucose units, the channels constraining the threaded portions of the guest polymer chains are ~0.5 and 1.0 nm in diameter and are separated by ~1.4 and 1.7 nm. This results in dense brushes with ~0.6 and 0.4 chains/nm2 (or 0.8 if two guest chains are included in each γ-CD channel) of the un-included portions of guest polymers emanating from the host CD-IC crystal surfaces. In addition, at least some of the guest chains leaving from a crystalline CD-IC surface re-enter another CD-IC crystal creating a network structure that leads to shape-memory behavior for (n-s)-polymer-CD-ICs. To some extent, (n-s)-polymer-CD-ICs can be considered as dense polymer brushes with chains that are tethered on both ends. Not surprisingly, the behavior of the un-included portions of the guest polymer chains in (n-s)-polymer-CD-ICs are quite different from those of their neat bulk samples, with higher glass-transition and melt crystallization temperatures and crystallinities. Here we additionally compare their behaviors to samples coalesced from their stoichiometric ICs, and more importantly to dense polymer brushes formed by polymer chains chemically bonded to surfaces at only one end. Judging on the basis of their glass-transition, crystallization and melting temperatures, and crystallinities, we generally find the un-included portions of chains in (n-s)-polymer-CD-ICs to be more constrained than those in neat bulk as-received and coalesced samples and in high density brushes. The last observation is likely because many of the un-included chain portions in (n-s)-polymer-CD-ICs are tethered/constrained at both ends, while the chains in their dense brushes are tethered at only one end.
A Cognitive Science View of Abhinavagupta’s Understanding of Consciousness
Authors: Biernacki Loriliai
Published: August 2014

This paper offers a comparative analysis of the nature of consciousness correlating the insights of the 11th century Śaiva philosopher Abhinavagupta with the work of some contemporary philosophers of consciousness. Ultimately these comparisons especially bring to light possibilities for constructing a materialist paradigm that might operate from a prioritization of subjectivity rather than objectivity. I propose that the Hindu, nondual Śaivite system that Abhinavagupta lays out offers a framework that may be useful for contemporary cognitive science and philosophy of mind precisely because Abhinavagupta offers a theory for connecting the material with the phenomenal.
Security Analysis and Improvements of Authentication and Access Control in the Internet of Things
Authors: Ndibanje Bruce , Lee Hoon-Jae , Lee Sang-Gon
Published: August 2014

Internet of Things is a ubiquitous concept where physical objects are connected over the internet and are provided with unique identifiers to enable their self-identification to other devices and the ability to continuously generate data and transmit it over a network. Hence, the security of the network, data and sensor devices is a paramount concern in the IoT network as it grows very fast in terms of exchanged data and interconnected sensor nodes. This paper analyses the authentication and access control method using in the Internet of Things presented by Jing et al. According to our analysis, Jing et al.’s protocol is costly in the message exchange and the security assessment is not strong enough for such a protocol. Therefore, we propose improvements to the protocol to fill the discovered weakness gaps. The protocol enhancements facilitate many services to the users such as user anonymity, mutual authentication, and secure session key establishment. Finally, the performance and security analysis show that the improved protocol possesses many advantages against popular attacks, and achieves better efficiency at low communication cost.
Acoustic Devices for Particle and Cell Manipulation and Sensing
Authors: Qiu Yongqiang , Wang Han , Demore Christine , Hughes David , Glynne-Jones Peter , Gebhardt Sylvia , Bolhovitins Aleksandrs , Poltarjonoks Romans , Weijer Kees , Schönecker Andreas , Hill Martyn , Cochran Sandy
Published: August 2014

An emerging demand for the precise manipulation of cells and particles for applications in cell biology and analytical chemistry has driven rapid development of ultrasonic manipulation technology. Compared to the other manipulation technologies, such as magnetic tweezing, dielectrophoresis and optical tweezing, ultrasonic manipulation has shown potential in a variety of applications, with its advantages of versatile, inexpensive and easy integration into microfluidic systems, maintenance of cell viability, and generation of sufficient forces to handle particles, cells and their agglomerates. This article briefly reviews current practice and reports our development of various ultrasonic standing wave manipulation devices, including simple devices integrated with high frequency (>20 MHz) ultrasonic transducers for the investigation of biological cells and complex ultrasonic transducer array systems to explore the feasibility of electronically controlled 2-D and 3-D manipulation. Piezoelectric and passive materials, fabrication techniques, characterization methods and possible applications are discussed. The behavior and performance of the devices have been investigated and predicted with computer simulations, and verified experimentally. Issues met during development are highlighted and discussed. To assist long term practical adoption, approaches to low-cost, wafer level batch-production and commercialization potential are also addressed.
The Dynamic Coordinated Development of a Regional Environment-Tourism-Economy System: A Case Study from Western Hunan Province, China
Authors: Yuan Yaoqing , Jin Maozhu , Ren Jinfei , Hu Mingming , Ren Peiyu
Published: August 2014

Based on regional coordination theory and system theory, the authors constructed an evaluation index system for the coordinated development of a regional environment-tourism-economy system with a pressure-state-response (PSR) model. With a coordinated development model, it further empirically analyzed the coordinated development state of an environment-tourism-economy system in western Hunan from 2001 to 2012. The results showed that, although this environment-tourism-economy system failed to achieve a high benefit index, inter-subsystem coupling extent, and coordinated development index, the three indices presented an increasing overall trend. This outcome suggested that the sub-systems in this system were developing towards their optimal proportions: the development of these sub-systems (environmental, tourism, and economic) was unbalanced in western Hunan. The environment therein sees only slow development although provided with a favorable ecological foundation. Economic development, which has long been lagging, acted as the main factor restricting the coordinated development of a regional environment-tourism-economy system. To promote its coordinated development in western Hunan, the following recommendations were proposed: strengthen the prediction and warnings on the evolution of the whole system; optimize the industry’s structure; reinforce environmental management.
Supramolecular Chirality: Solvent Chirality Transfer in Molecular Chemistry and Polymer Chemistry
Authors: Fujiki Michiya
Published: August 2014

Controlled mirror symmetry breaking arising from chemical and physical origin is currently one of the hottest issues in the field of supramolecular chirality. The dynamic twisting abilities of solvent molecules are often ignored and unknown, although the targeted molecules and polymers in a fluid solution are surrounded by solvent molecules. We should pay more attention to the facts that mostly all of the chemical and physical properties of these molecules and polymers in the ground and photoexcited states are significantly influenced by the surrounding solvent molecules with much conformational freedom through non-covalent supramolecular interactions between these substances and solvent molecules. This review highlights a series of studies that include: (i) historical background, covering chiral NaClO3 crystallization in the presence of d-sugars in the late 19th century; (ii) early solvent chirality effects for optically inactive chromophores/fluorophores in the 1960s–1980s; and (iii) the recent development of mirror symmetry breaking from the corresponding achiral or optically inactive molecules and polymers with the help of molecular chirality as the solvent use quantity.
Synthesis and Analgesic Effects of μ-TRTX-Hhn1b on Models of Inflammatory and Neuropathic Pain
Authors: Liu Yu , Tang Jianguang , Zhang Yunxiao , Xun Xiaohong , Tang Dongfang , Peng Dezheng , Yi Jianming , Liu Zhonghua , Shi Xiaoliu
Published: August 2014

μ-TRTX-Hhn1b (HNTX-IV) is a 35-amino acid peptide isolated from the venom of the spider, Ornithoctonus hainana. It inhibits voltage-gated sodium channel Nav1.7, which has been considered as a therapeutic target for pain. The goal of the present study is to elucidate the analgesic effects of synthetic μ-TRTX-Hhn1b on animal models of pain. The peptide was first synthesized and then successfully refolded/oxidized. The synthetic peptide had the same inhibitory effect on human Nav1.7 current transiently expressed in HEK 293 cells as the native toxin. Furthermore, the analgesic potentials of the synthetic peptide were examined on models of inflammatory pain and neuropathic pain. μ-TRTX-Hhn1b produced an efficient reversal of acute nociceptive pain in the abdominal constriction model, and significantly reduced the pain scores over the 40-min period in the formalin model. The efficiency of μ-TRTX-Hhn1b on both models was equivalent to that of morphine. In the spinal nerve model, the reversal effect of μ-TRTX-Hhn1b on allodynia was longer and higher than mexiletine. These results demonstrated that μ-TRTX-Hhn1b efficiently alleviated acute inflammatory pain and chronic neuropathic pain in animals and provided an attractive template for further clinical analgesic drug design.
Protective Antigen-Specific Memory B Cells Persist Years after Anthrax Vaccination and Correlate with Humoral Immunity
Authors: Garman Lori , Smith Kenneth , Farris A. , Nelson Michael , Engler Renata , James Judith
Published: August 2014

Anthrax Vaccine Adsorbed (AVA) generates short-lived protective antigen (PA) specific IgG that correlates with in vitro toxin neutralization and protection from Bacillus anthracis challenge. Animal studies suggest that when PA-specific IgG has waned, survival after spore challenge correlates with an activation of PA-specific memory B cells. Here, we characterize the quantity and the longevity of AVA-induced memory B cell responses in humans. Peripheral blood mononuclear cells (PBMCs) from individuals vaccinated ≥3 times with AVA (n = 50) were collected early (3–6 months, n = 27) or late after their last vaccination (2–5 years, n = 23), pan-stimulated, and assayed by ELISPOT for total and PA-specific memory B cells differentiated into antibody secreting cells (ASCs). PA-specific ASC percentages ranged from 0.02% to 6.25% (median: 1.57%) and did not differ between early and late post-vaccination individuals. PA-specific ASC percentages correlated with plasma PA-specific IgG (r = 0.42, p = 0.03) and toxin neutralization (r = 0.52, p = 0.003) early post vaccination. PA-specific ASC percentages correlated with supernatant anti-PA both early (r = 0.60, p = 0.001) and late post vaccination (r = 0.71, p < 0.0001). These data suggest PA-specific memory B cell responses are long-lived and can be estimated after recent vaccination by the magnitude and neutralization capacity of the humoral response.
Improved Energy Recovery by Anaerobic Grey Water Sludge Treatment with Black Water
Authors: Tervahauta Taina , Bryant Isaac , Leal Lucía , Buisman Cees , Zeeman Grietje
Published: August 2014

This study presents the potential of combining anaerobic grey water sludge treatment with black water in an up-flow anaerobic sludge blanket (UASB) reactor to improve energy recovery within source-separated sanitation concepts. Black water and the mixture of black water and grey water sludge were compared in terms of biochemical methane potential (BMP), UASB reactor performance, chemical oxygen demand (COD) mass balance and methanization. Grey water sludge treatment with black water increased the energy recovery by 23% in the UASB reactor compared to black water treatment. The increase in the energy recovery can cover the increased heat demand of the UASB reactor and the electricity demand of the grey water bioflocculation system with a surplus of 0.7 kWh/cap/y electricity and 14 MJ/cap/y heat. However, grey water sludge introduced more heavy metals in the excess sludge of the UASB reactor and might therefore hinder its soil application.