bluuebunny/crossref_metadata_2025 huggingface dataset:
Systemic Crop Signaling for Automatic Recognition of Transplanted Lettuce and Tomato under Different Levels of Sunlight for Early Season Weed Control
Authors: Su Wen-Hao
Published: September 2020

Conventional cultivation works to control weeds between the rows, but it ignores the weeds in crop rows which are most competitive with crops. Many vegetable crops still require manual removal of intra-row weeds not otherwise controlled by herbicides or conventional cultivation. The increasing labor costs of weed control and the continued emergences of herbicide-resistant weeds are threatening grower ability to manage weeds and maintain profitability. Intra-row weeders are commercially available but work best in low weed populations. One strategy for rapid weed crop differentiation is to utilize a machine-detectable compound to mark a crop. This paper proposes a new systemic plant signaling technology that can create machine-readable crops to facilitate the automated removal of intra-row weeds in early growth stages. Rhodamine B (Rh–B) is an efficient systemic compound to label crop plants due to its membrane permeability and unique fluorescent properties. The project involves applying solutions of Rh–B at 60 ppm to the roots of lettuce and tomato plants prior to transplantation to evaluate Rh–B persistence in plants under different levels of sunlight. Lettuce and tomato seedlings with the systemic Rh–B should be reliably recognized during their early growth stages. An intelligent robot is expected to be developed to identify the locations of plants based on the systemic signal inside. Reduced light treatments should help to alleviate the photodegradation of Rh–B in plants. After being exposed to full sunlight for 27 days, the systemic Rh–B would be detectable in tomato branches and lettuce ribs, and these plants are tolerant to root treatments with this fluorescent compound. This paper describes the project background and plan as well as the anticipated contributions of the research to allow the machine vision system to reliably identify the crop plants, and thus showing technical feasibility for outdoor weed control.
Secondary and Tertiary Voltage Control of a Multi-Region Power System
Authors: Abdalla Omar H. , Fayek Hady H. , Abdel Ghany Abdel Ghany M.
Published: September 2020

This paper presents techniques for the application of tertiary and secondary voltage control through the use of intelligent proportional integral derivative (PID) controllers and the wide area measurement system (WAMS) in the IEEE 39 bus system (New England system). The paper includes power system partitioning, pilot bus selection, phasor measurement unit (PMU) placement, and optimal secondary voltage control parameter calculations to enable the application of the proposed voltage control. The power system simulation and analyses were performed using the DIgSILENT and MATLAB software applications. The optimal PMU placement was performed in order to apply secondary voltage control. The tertiary voltage control was performed through an optimal power flow optimization process in order to minimize the active power losses. Two different methods were used to design the PID secondary voltage control, namely, genetic algorithm (GA) and neural network based on genetic algorithm (NNGA). A comparison of system performances using these two methods under different operating conditions is presented. The results show that NNGA secondary PID controllers are more robust than GA ones. The paper also presents a comparison between system performance with and without secondary voltage control, in terms of voltage deviation index and total active power losses. The graph theory is used in system partitioning, and sensitivity analysis is used in pilot bus selection, the results of which proved their effectiveness.
Low Cost Circularly Polarized Antenna for IoT Space Applications
Authors: Trinh Le Huy , Truong Nguyen Vu , Ferrero Fabien
Published: September 2020

This work presents the use of a three-element radiating structure for circularly polarized Low-Power Wide Area Network (LP-WAN) communication with space. The proposed structure has a 72 mm × 72 mm × 12 mm compact size with Right-Handed Circular Polarization (RHCP) and a 120∘ wide beamwidth radiation pattern. Printed on low-cost FR4 Epoxy substrate, a feeding network circuit based on Quasi Lumped Quadrature Coupler (QLQC), it achieves a −0.6 dB insertion loss and a very compact size. The final structure has a 69% total efficiency and a 3.14 dBic realized gain.
The Way of Macroplastic through the Environment
Authors: Lechthaler Simone , Waldschläger Kryss , Stauch Georg , Schüttrumpf Holger
Published: September 2020

With the focus on microplastic in current research, macroplastic is often not further considered. Thus, this review paper is the first to analyse the entry paths, accumulation zones, and sinks of macroplastic in the aquatic, terrestrial, and atmospheric environment by presenting transport paths and concentrations in the environment as well as related risks. This is done by applying the Source–Pathway–Receptor model on macroplastic in the environment. Based on this model, the life cycle of macroplastic is structurally described, and knowledge gaps are identified. Hence, current research aspects on macroplastic as well as a sound delimitation between macro- and microplastic that can be applied to future research are indicated. The results can be used as basic information for further research and show a qualitative assessment of the impact of macroplastic that ends up in the environment and accumulates there. Furthermore, the applied model allows for the first time a quantitative and structured approach to macroplastic in the environment.
Improvement of the Shelf-Life Status of Modified Atmosphere Packaged Camel Meat Using Nisin and Olea europaea Subsp. laperrinei Leaf Extract
Authors: Djenane Djamel , Aboudaou Malek , Djenane Fatiha , García-Gonzalo Diego , Pagán Rafael
Published: September 2020

The impact of combined biopreservation treatment with Olea europaea subsp. laperrinei leave extracts (laper.OLE) and nisin on the quality attributes of camel steaks packaged under high O2 (80%) and CO2 (20%) atmosphere was investigated during refrigerated (1 ± 1 °C) long-term storage. As measured by reversed phase HPLC/DAD analysis, oleuropein is the phenolic compound most present in the chemical composition of laper.OLE (63.03%). Camel steaks treated with laper.OLE had a lower concentration of thiobarbituric acid-reactive substances (TBA-RSs) in the course of 30 days of storage. Surface metmyoglobin (MetMb) increased at a reduced rate in laper.OLE-treated samples compared to control samples. Neither modified atmosphere packaging (MAP) nor biopreservation treatments significantly altered the tenderness of camel steaks, expressed in terms of Warner-Bratzler shear force (WBSF), as compared to control samples. After 30 days of storage, psychrotrophic bacteria and Pseudomonas spp. counts were significantly lower in camel steaks treated with a combination of laper.OLE and nisin than in untreated steaks. Moreover, samples treated with laper.OLE received higher scores on bitterness acceptability. In sum, the use of combined biopreservation methods could be a sustainable solution for the preservation and promotion of the quality characteristics of camel meat in arid regions.
Potential Use of Silica Nanoparticles for the Microbial Stabilisation of Wine: An In Vitro Study Using Oenococcus oeni as a Model
Authors: Pachnowska Kamila , Cendrowski Krzysztof , Stachurska Xymena , Nawrotek Paweł , Augustyniak Adrian , Mijowska Ewa
Published: September 2020

The emerging trend towards the reduction of SO2 in winemaking has created a need to look for alternative methods to ensure the protection of wine against the growth of undesired species of microorganisms and to safely remove wine microorganisms. This study describes the possible application of silica nanospheres as a wine stabilisation agent, with Oenococcus oeni (DSM7008) as a model strain. The experiment was conducted firstly on model solutions of phosphate-buffered saline and 1% glucose. Their neutralising effect was tested under stirring with the addition of SiO2 (0.1, 0.25, and 0.5 mg/mL). Overall, the highest concentration of nanospheres under continuous stirring resulted in the greatest decrease in cell counts. Transmission electron microscope (TEM) and scanning electron microscopy (SEM) analyses showed extensive damage to the bacterial cells after stirring with silica nanomaterials. Then, the neutralising effect of 0.5 mg/mL SiO2 was tested in young red wine under stirring, where cell counts were reduced by over 50%. The obtained results suggest that silica nanospheres can serve as an alternative way to reduce or substitute the use of sulphur dioxide in the microbial stabilisation of wine. In addition, further aspects of following investigations should focus on the protection against enzymatic and chemical oxidation of wine.
Phenolome of Asian Agrimony Tea (Agrimonia asiatica Juz., Rosaceae): LC-MS Profile, α-Glucosidase Inhibitory Potential and Stability
Authors: I. Kashchenko Nina , Olennikov Daniil N.
Published: September 2020

Functional beverages constitute the rapidly increasing part of the functional food section and represent an area with a wide range of products including herbal-based beverages. We carried out screening investigations of the extracts of 85 Rosaceous tea plants. Among the extracts analyzed Agrimonia asiatica herb extract demonstrated the highest inhibitory activity against the enzyme α-glucosidase (20.29 µg/mL). As a result of chromato-mass-spectrometric profiling of A. asiatica herb with high-performance liquid chromatography with photodiode array and electrospray triple quadrupole mass-spectrometric detection (HPLC-PDA-ESI-tQ-MS) 60 compounds were identified, including catechins, ellagitannins, flavones, flavonols, gallotannins, hydroxycinnamates, procyanidins, most for the very first time. The analysis of the seasonal variation of metabolites in A. asiatica herb demonstrated that the phenolic content was highest in summer samples and lower in spring and autumn. HPLC activity-based profiling was utilized to identify compounds of A. asiatica herb with the maximal α-glucosidase inhibitory activity. The most pronounced inhibition of α-glucosidase was observed for agrimoniin, while less significant results of inhibition were revealed for ellagic acid and isoquercitrin. The evaluation of phenolic content in A. asiatica herbal teas with the subsequent determination of α-glucosidase inhibiting potential was discovered. Maximum inhibition of α-glucosidase was observed for hot infusion (75.33 µg/mL) and the minimum for 30 min decoction (159.14 µg/mL). Our study demonstrated that A. asiatica herbal tea is a prospective functional beverage in which dietary intake may help to reduce blood glucose.
Annona cherimola Miller Fruit as a Promising Candidate against Diabetic Complications: An In Vitro Study and Preliminary Clinical Results
Authors: Vasarri Marzia , Barletta Emanuela , Vinci Santina , Ramazzotti Matteo , Francesconi Andrea , Manetti Francesco , Degl’Innocenti Donatella
Published: September 2020

Diabetes is a chronic metabolic disease with a strong social impact worldwide. Under chronic hyperglycemia, protein glycation strongly contributes to diabetes-related complications onset. Anti-glycation agents and inhibitors of α-glucosidase are often therapeutically used to control postprandial glycemia in order to prevent development of long-term diabetic complications. Given drug resistance and adverse effects of conventional antidiabetic therapies, the discovery of new effective and non-toxic naturally occurring compounds is needed to prevent and/or to manage life-threatening diabetic complications. Annona cherimola Miller fruit has been used in Mexican traditional medicine as natural remedy against diabetes. In this work, the in vitro anti-glycation and anti-α-glucosidase roles of Annona cherimola Miller pulp extract (CE) were investigated. Moreover, healthy and diabetic subjects were enrolled in a cross-over design intervention study aimed at investigating the effects of pulp intake on postprandial glycemia. This work shows that CE was able to inhibit albumin glycation in vitro and to inhibit α-glucosidase enzyme. Furthermore, the pulp intake did not contribute to an increase in postprandial glycemia, making it a suitable source of health-promoting phytonutrients and a potential functional food in diabetics and pre-diabetics diet.
Principal Component Analysis from Mass Spectrometry Data Combined to a Sensory Evaluation as a Suitable Method for Assessing Bitterness of Enzymatic Hydrolysates Produced from Micellar Casein Proteins
Authors: Daher Dahlia , Deracinois Barbara , Baniel Alain , Wattez Elodie , Dantin Justine , Froidevaux Renato , Chollet Sylvie , Flahaut Christophe
Published: September 2020

Enzymatic hydrolysis of food proteins generally changes the techno-functional, nutritional, and organoleptic properties of hydrolyzed proteins. As a result, protein hydrolysates have an important interest in the food industries. However, they tend to be characterized by a bitter taste and some off-flavors, which limit their use in the food industry. These tastes and aromas come from peptides, amino acids, and volatile compounds generated during hydrolysis. In this article, sixteen more or less bitter enzymatic hydrolysates produced from a milk protein liquid fraction enriched in micellar caseins using commercially available, food-grade proteases were subjected to a sensory analysis using a trained and validated sensory panel combined to a peptidomics approach based on the peptide characterization by reverse-phase high-performance liquid chromatography, high-resolution mass spectrometry, and bioinformatics software. The comparison between the sensory characteristics and the principal components of the principal component analysis (PCA) of mass spectrometry data reveals that peptidomics constitutes a convenient, valuable, fast, and economic intermediate method to evaluating the bitterness of enzymatic hydrolysates, as a trained sensory panel can do it.
Beyond Back Splicing, a Still Poorly Explored World: Non-Canonical Circular RNAs
Authors: Robic Annie , Kühn Christa
Published: September 2020

Most of the circRNAs reported to date originate from back splicing of a pre-mRNA, and these exonic circRNAs are termed canonical circRNAs. Our objective was to provide an overview of all other (non-canonical) circRNAs that do not originate from the junction of two exons and to characterize their common properties. Those generated through a failure of intron lariat debranching are the best known, even though studies on them are rare. These circRNAs retain the 2′–5′ bond derived from the intron lariat, and this feature probably explains the difficulties in obtaining efficient reverse transcription through the circular junction. Here, we provide an unprecedented overview of non-canonical circRNAs (lariat-derived intronic circRNAs, sub-exonic circRNAs, intron circles, tricRNAs), which all derive from non-coding sequences. As there are few data suggesting their involvement in cellular regulatory processes, we believe that it is early to propose a general function for circRNAs, even for lariat-derived circRNAs. We suggest that their small size and probably strong secondary structures could be major obstacles to their reliable detection. Nevertheless, we believe there are still several possible ways to advance our knowledge of this class of non-coding RNA.
Delivery Approaches for Therapeutic Genome Editing and Challenges
Authors: Ates Ilayda , Rathbone Tanner , Stuart Callie , Bridges P. Hudson , Cottle Renee N.
Published: September 2020

Impressive therapeutic advances have been possible through the advent of zinc-finger nucleases and transcription activator-like effector nucleases. However, discovery of the more efficient and highly tailorable clustered regularly interspaced short palindromic repeats (CRISPR) and associated proteins (Cas9) has provided unprecedented gene-editing capabilities for treatment of various inherited and acquired diseases. Despite recent clinical trials, a major barrier for therapeutic gene editing is the absence of safe and effective methods for local and systemic delivery of gene-editing reagents. In this review, we elaborate on the challenges and provide practical considerations for improving gene editing. Specifically, we highlight issues associated with delivery of gene-editing tools into clinically relevant cells.
A Pan-Genome Guided Metabolic Network Reconstruction of Five Propionibacterium Species Reveals Extensive Metabolic Diversity
Authors: McCubbin Tim , Gonzalez-Garcia R. Axayacatl , Palfreyman Robin W. , Stowers Chris , Nielsen Lars K. , Marcellin Esteban
Published: September 2020

Propionibacteria have been studied extensively since the early 1930s due to their relevance to industry and importance as human pathogens. Still, their unique metabolism is far from fully understood. This is partly due to their signature high GC content, which has previously hampered the acquisition of quality sequence data, the accurate annotation of the available genomes, and the functional characterization of genes. The recent completion of the genome sequences for several species has led researchers to reassess the taxonomical classification of the genus Propionibacterium, which has been divided into several new genres. Such data also enable a comparative genomic approach to annotation and provide a new opportunity to revisit our understanding of their metabolism. Using pan-genome analysis combined with the reconstruction of the first high-quality Propionibacterium genome-scale metabolic model and a pan-metabolic model of current and former members of the genus Propionibacterium, we demonstrate that despite sharing unique metabolic traits, these organisms have an unexpected diversity in central carbon metabolism and a hidden layer of metabolic complexity. This combined approach gave us new insights into the evolution of Propionibacterium metabolism and led us to propose a novel, putative ferredoxin-linked energy conservation strategy. The pan-genomic approach highlighted key differences in Propionibacterium metabolism that reflect adaptation to their environment. Results were mathematically captured in genome-scale metabolic reconstructions that can be used to further explore metabolism using metabolic modeling techniques. Overall, the data provide a platform to explore Propionibacterium metabolism and a tool for the rational design of strains.
Mechanisms of Photoreceptor Death in Retinitis Pigmentosa
Authors: Newton Fay , Megaw Roly
Published: September 2020

Retinitis pigmentosa (RP) is the most common cause of inherited blindness and is characterised by the progressive loss of retinal photoreceptors. However, RP is a highly heterogeneous disease and, while much progress has been made in developing gene replacement and gene editing treatments for RP, it is also necessary to develop treatments that are applicable to all causative mutations. Further understanding of the mechanisms leading to photoreceptor death is essential for the development of these treatments. Recent work has therefore focused on the role of apoptotic and non-apoptotic cell death pathways in RP and the various mechanisms that trigger these pathways in degenerating photoreceptors. In particular, several recent studies have begun to elucidate the role of microglia and innate immune response in the progression of RP. Here, we discuss some of the recent progress in understanding mechanisms of rod and cone photoreceptor death in RP and summarise recent clinical trials targeting these pathways.
Mouse Models of Human Pathogenic Variants of TBC1D24 Associated with Non-Syndromic Deafness DFNB86 and DFNA65 and Syndromes Involving Deafness
Authors: Tona Risa , Lopez Ivan A. , Fenollar-Ferrer Cristina , Faridi Rabia , Anselmi Claudio , Khan Asma A. , Shahzad Mohsin , Morell Robert J. , Gu Shoujun , Hoa Michael , Dong Lijin , Ishiyama Akira , Belyantseva Inna A. , Riazuddin Sheikh , Friedman Thomas B.
Published: September 2020

Human pathogenic variants of TBC1D24 are associated with clinically heterogeneous phenotypes, including recessive nonsyndromic deafness DFNB86, dominant nonsyndromic deafness DFNA65, seizure accompanied by deafness, a variety of isolated seizure phenotypes and DOORS syndrome, characterized by deafness, onychodystrophy, osteodystrophy, intellectual disability and seizures. Thirty-five pathogenic variants of human TBC1D24 associated with deafness have been reported. However, functions of TBC1D24 in the inner ear and the pathophysiology of TBC1D24-related deafness are unknown. In this study, a novel splice-site variant of TBC1D24 c.965 + 1G > A in compound heterozygosity with c.641G > A p.(Arg214His) was found to be segregating in a Pakistani family. Affected individuals exhibited, either a deafness-seizure syndrome or nonsyndromic deafness. In human temporal bones, TBC1D24 immunolocalized in hair cells and spiral ganglion neurons, whereas in mouse cochlea, Tbc1d24 expression was detected only in spiral ganglion neurons. We engineered mouse models of DFNB86 p.(Asp70Tyr) and DFNA65 p.(Ser178Leu) nonsyndromic deafness and syndromic forms of deafness p.(His336Glnfs*12) that have the same pathogenic variants that were reported for human TBC1D24. Unexpectedly, no auditory dysfunction was detected in Tbc1d24 mutant mice, although homozygosity for some of the variants caused seizures or lethality. We provide some insightful supporting data to explain the phenotypic differences resulting from equivalent pathogenic variants of mouse Tbc1d24 and human TBC1D24.
How Will COVID-19 Impact on the Governance of Global Health in the 2030 Agenda Framework? The Opinion of Experts
Authors: Fernández-Portillo Luis A. , Sianes Antonio , Santos-Carrillo Francisco
Published: September 2020

In 2015, the 2030 Agenda was formally adopted by the United Nations, establishing a set of 17 Sustainable Development Goals (SDGs). SDG 3 seeks to promote Global Health and the quality of public health systems in developing countries. The achievement of these goals requires the commitment of all signing countries, but the COVID-19 crisis is changing the behavior of the main stakeholders in the international arena. What do the experts think about these changes? Could these changes threaten the 2030 Agenda and Global Health? To answer these questions, we conduct a content analysis of 152 documents written by experts from the 15 main think tanks on international development policy. The results point out that the new scenario brought about by the pandemic is hindering the necessary cooperation between countries to achieve global health goals and to guarantee public health coverage in developing countries. To deal with these challenges, more delegation of powers to international organizations and a reform of the international cooperation system are needed. With this analysis, we launch a warning about potential weaknesses of the institutional design of the 2030 Agenda in order for it to survive in a post-COVID-19 world and remain a valid instrument to promote health worldwide.
Vulnerability Factors Associated with Lifetime Posttraumatic Stress Disorder among Veterans 40 Years after War
Authors: Castro-Vale Ivone , Severo Milton , Carvalho Davide , Mota-Cardoso Rui
Published: September 2020

Vulnerability factors for posttraumatic stress disorder (PTSD) development are still controversial. Our aim was to study the vulnerability factors for the development of war-related PTSD over a period of 40 years after exposure. A cross-sectional, observational study was carried out on 61 male traumatized war veterans, taking into consideration adverse childhood experiences (ACE), attachment orientations, number of non-war-related traumatic events, and war experiences. Lifetime PTSD was assessed by using the Clinician-Administered PTSD Scale. Insecure attachment styles were significantly associated with lifetime PTSD and even after adjustment for war exposure this was still significant. Non-war-related traumatic events were not associated with lifetime PTSD, whereas ACE were associated with lifetime PTSD. War-related experiences were also associated with lifetime PTSD, except for injury or disease. The results for our sample show that, 40 years after war, the intensity of war-related experiences and ACE were significantly and independently associated with the development of lifetime PTSD. Insecure attachment was significantly associated with lifetime PTSD, which, in turn, are both positively associated with war exposure. These findings may have implications for patient care, as they constitute a strong argument that attachment-focused therapies could well be necessary 40 years after trauma.
Facilitators and Barriers Surrounding the Role of Administration in Employee Job Satisfaction in Long-Term Care Facilities: A Systematic Review
Authors: Lee Kimberly , Mileski Michael , Fohn Joanna , Frye Leah , Brooks Lisa
Published: September 2020

Previous literature has shown how associate engagement has positively impacted on productivity, job satisfaction, safety, retention, consumer sentiment, and financial performance in hospitals and healthcare systems. However, a lack of research showing the relationship between associate engagement and job satisfaction within the long-term care environment has existed. Our objective was to investigate characteristics within the long-term care environment that promote and detract from associate job satisfaction and extrapolate the best practices in maintaining job satisfaction and engagement. This systematic review queried CINAHL, PubMed (MEDLINE), and Academic Search Ultimate databases for peer-reviewed publications for facilitators and barriers commensurate with employee job satisfaction in long-term care facilities using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) and the Kruse Protocols. The authors identified 11 facilitators for job satisfaction and 18 barriers to job satisfaction in the 60 selected articles. The top four facilitators were Supportive Leadership, Capable and Motivated Employees, Positive Organizational Values, and Social Support Mechanisms. The top four barriers were condescending management style, high job demands, lack of self-care, and lack of training with medically complex patients. The systematic review revealed the importance of maintaining satisfied employees in the long-term care workplace through am emphasis leadership and on the facilitators identified to best serve their associates and improve care for residents.
The Next Step for MRD in Myeloma? Treating MRD Relapse after First Line Treatment in the REMNANT Study
Authors: Rasmussen Anne-Marie , Askeland Frida Bugge , Schjesvold Fredrik
Published: September 2020

The treatment approach for multiple myeloma (MM) has changed in recent years. After the approval of maintenance treatment after stem cell transplant in younger patients, the paradigm of continuous treatment is now prevailing in all clinical situations of myeloma. However, the best time to initiate relapse treatment is still unclear. With increased frequency of minimal residual disease (MRD) negativity, and the established clinical benefit of this finding, one of the large clinical questions in myeloma is how to approach MRD re-appearance. In this paper, we go through the MRD technology, existing and possible uses of MRD in the clinic, and data for early treatment before we introduce the design of the ongoing REMNANT study; a randomized study with early treatment of MRD relapse after first line treatment.
Knowledge Assessment of COVID-19 Symptoms: Gender Differences and Communication Routes for the Generation Z Cohort
Authors: Kamenidou Irene (Eirini) , Stavrianea Aikaterini , Mamalis Spyridon , Mylona Ifigeneia
Published: September 2020

This paper explores the generation Z (Gen Z) cohort’s self-assessed knowledge regarding the coronavirus disease 2019 (COVID-19) symptoms as well as their interest in acquiring information and learning more about the transmission and spread of the severe acute respiratory syndrome coronavirus 2 (SARS-COV-2 virus) and the COVID-19 symptoms. Additionally, it investigates gender differences in self-assessed knowledge of COVID-19 symptoms. Field research employing a nonprobability sampling method with an online questionnaire resulted in collecting 762 valid questionnaires. Data analysis included descriptive statistics, factor and reliability analysis, and the independent sample t-test. Results reveal that overall symptom knowledge was assessed higher than the self-assessed knowledge of the 13 specific symptoms. No gender differences were detected regarding self-assessed knowledge of the following COVID-19 symptoms: cough, dyspnea, anorexia, productive cough with expectoration (phlegm), headache, and diarrhea. On the other hand, for self-assessed overall knowledge of COVID-19 symptoms, as well as self-assessed knowledge of COVID-19 symptoms related to fever and fatigue, myalgia (muscle pain), pharyngodynia, nausea–vomitus, hemoptysis, and abdominal pain, the t-tests conducted showed that there are statistical differences in knowledge assessment between male and female subjects. Based on the outcomes, the paper provides marketing communication practices targeting this young generation cohort to raise awareness so that Gen Z’ers may react effectively if these symptoms are observed and, thus, request medical assistance.
Environmental Kuznets Curve: Empirical Relationship between Energy Consumption and Economic Growth in Upper-Middle-Income Regions of China
Authors: Li Shixiang , Shi Jianru , Wu Qiaosheng
Published: September 2020

This paper examines the environmental Kuznets curve (EKC) relationship between energy consumption and economic growth in upper-middle-income regions of China with the panel data of 21 provinces from 2000 to 2017. The influence mechanism of socio-economic factors on the EKC of these regions is also detected. The results show that the energy consumption EKC fitting map in these regions conforms to the classical environmental Kuznets curve, which is an inverted “N” type, and the inflection point is ahead and more accurate after adding spatial effects. The direct effect of energy consumption has delayed the inflection point, indicating that the level of industrialization, urbanization, and population density have a significant impact on EKC. At the same time, it is found that the level of industrialization and population density have a positive relationship with energy consumption, while the level of urbanization has a negative correlation with energy consumption. The spatial spillover effect of the indirect effects of total energy consumption, coal consumption, and crude oil consumption shows that the level of industrialization has a significant and negative link with EKC. The increase in the level of industrialization will affect the total energy consumption of neighboring areas and the consumption of coal and crude oil.
A Qualitative Research Study Which Explores Humanitarian Stakeholders’ Views on Healthcare Access for Refugees in Greece
Authors: Joseph Liz , Ismail Sharif A. , Gunst Meghan , Jarman Kate , Prior Dina , Harris Matthew , Abbara Aula
Published: September 2020

Introduction: As of January 2020, 115,600 refugees remain in Greece; most are Afghani, Iraqi or Syrian nationals. This qualitative research study explores the views of key stakeholders providing healthcare for refugees in Greece between 2015 and 2018. The focus was on identifying key barriers and facilitators to healthcare access for refugees in Greece. Methods: 16 interviewees from humanitarian and international organisations operating in Greece were identified through purposive and snowball sampling. Semi-structured interviews were conducted between March and April 2018. Data were analysed using the Framework Method. Results: Key themes affecting healthcare access included the influence of socio-cultural factors (healthcare expectations, language, gender) and the ability of the Greek health system to respond to existing and evolving demands; these included Greece’s ongoing economic crisis, human resource shortages, weak primary healthcare system, legal barriers and logistics. The evolution of the humanitarian response from emergency to sustained changes to EU funding, coordination and comprehensiveness of services affected healthcare access for refugees. Conclusion: The most noted barriers cited by humanitarian stakeholders to healthcare access for refugees in Greece were socio-cultural and language differences between refugees and healthcare providers and poor coordination among stakeholders. Policies and interventions which address these could improve healthcare access for refugees in Greece with coordination led by the EU.
Testing the Effects of COVID-19 Confinement in Spanish Children: The Role of Parents’ Distress, Emotional Problems and Specific Parenting
Authors: Romero Estrella , López-Romero Laura , Domínguez-Álvarez Beatriz , Villar Paula , Gómez-Fraguela Jose Antonio
Published: September 2020

The present study aimed to examine the effects of the Spanish confinement derived from the COVID-19 crisis on children and their families, accounting for child’s age. A range of child negative (e.g., conduct problems) and positive outcomes (e.g., routine maintenance) were examined, along with a set of parent-related variables, including resilience, perceived distress, emotional problems, parenting distress and specific parenting practices (e.g., structured or avoidant parenting), which were modeled through path analysis to better understand child adjustment. Data were collected in April 2020, with information for the present study provided by 940 (89.6%) mothers, 102 (9.7%) fathers and 7 (0.7%) different caregivers, who informed on 1049 Spanish children (50.4% girls) aged 3 to 12 years (Mage = 7.29; SD = 2.39). The results suggested that, according to parents’ information, most children did not show important changes in behavior, although some increasing rates were observed for both negative and positive outcomes. Child adjustment was influenced by a chain of effects, derived from parents’ perceived distress and emotional response to the COVID-19 crisis, via parenting distress and specific parenting practices. While parenting distress in particular triggered child negative outcomes, specific parenting practices were more closely related to child positive outcomes. These findings may help to better inform, for potential future outbreaks, effective guidelines and prevention programs aimed at promoting the child’s well-being in the family.
Industrial Structure Adjustment and Regional Green Development from the Perspective of Mineral Resource Security
Authors: Zheng You , Xiao Jianzhong , Cheng Jinhua
Published: September 2020

Mineral resource security is the premise and foundation of the regional green rise strategy. And the adjustment of industrial structure is an effective way to relieve the pressure of the current green economy transformation. Based on the Shift-share Method and the Spatial Durbin model, this paper takes 30 regions in China from 2006 to 2017 as examples to study the impact of industrial structure adjustment on China’s green development from the perspective of mineral resource security. The empirical results show that: China is still in the process of industrial transfer. The dynamic effect of industrial structure promotes green development from the perspective of mineral resource security, while its static effect inhibits green development from the perspective of mineral resource security. The spatial spillover effect of the industrial structure affecting green development from the perspective of mineral resource security is significant. The static structural effect of the tertiary industry promotes the green development of the region, and it has a significant negative impact on neighboring areas, while the secondary industry’s static structural effect has the opposite effect.
Barriers and Facilitators in the Strengthening Families Program (SFP 10–14) Implementation Process in Northeast Brazil: A Retrospective Qualitative Study
Authors: Abdala Ingrid Gomes , Murta Sheila Giardini , Menezes Jordana Calil Lopes de , Nobre-Sandoval Larissa de Almeida , Gomes Maria do Socorro Mendes , Duailibe Karina Damous , Farias Danielle Aranha
Published: September 2020

This study analyzed contextual barriers and facilitators in the implementation of Strengthening Families Program (SFP 10–14), Brazilian version, a family-based preventive program focused on the prevention of risk behaviors for adolescent health. SFP 10–14 was implemented between 2016 and 2017 for socioeconomically vulnerable families in four Northeast Brazilian states as a tool of the National Drug Policy. A retrospective qualitative study was carried out in which 26 implementation agents participated. Data from 16 individual interviews and two group interviews were analyzed through content analysis. The most recurrent barriers were the group facilitators’ working conditions, weak municipal administration, precarious infrastructure, inadequate group facilitator training methodologies, low adherence of managers and professionals, and funding scarcity. The conditions highlighted as favorable to the implementation were proper intersectoral coordination, engagement of involved actors, awareness of public agency administrators, municipal management efficacy, and efficient family recruitment strategies. Favorable political contexts, engagement of implementation agents, and intersectoral implementation strategies were identified as central to the success of the implementation of SFP 10–14, especially in the adoption of the intervention, community mobilization, and intervention delivery stages. Further studies should combine contexts, mechanisms, and results for a broad understanding of the effectiveness of this intervention in the public sector.
The Impact of COVID-19 on Mental Health: The Role of Locus on Control and Internet Use
Authors: Sigurvinsdottir Rannveig , Thorisdottir Ingibjorg E. , Gylfason Haukur Freyr
Published: September 2020

The true extent of the mental health implications of the COVID-19 pandemic are unclear, but early evidence suggests poorer mental health among those exposed to the pandemic. The Internet may have differential effects, by both connecting people with resources, or reinforce the constant checking of negative information. Moreover, locus of control becomes important in an uncontrollable pandemic. The current study aimed to examine whether exposure to COVID-19 would relate to greater symptoms of depression, anxiety and stress, and to examine the role of internet use and locus of control. Adults in the United States and five European countries (N = 1723) answered an online survey through the website Mturk. Results show elevated psychological symptoms among those who have become infected with COVID-19 or perceive themselves to be at high risk if infected. Experience using the Internet relates to fewer symptoms, but information seeking is associated with more symptoms. Internet social capital relates to fewer symptoms of depression. Having an external locus of control relates to greater symptoms. These findings suggest that public health officials need to focus on the mental health effects of the pandemic, and that internet use and locus of control could be targets to improve mental health in the population.
A Systematic Review of Job Demands and Resources Associated with Compassion Fatigue in Mental Health Professionals
Authors: Singh Jasmeet , Karanika-Murray Maria , Baguley Thom , Hudson John
Published: September 2020

Psychosocial hazards in mental healthcare contribute to the development of compassion fatigue in mental health professionals. Compassion fatigue has a negative impact on the mental health and wellbeing of professionals that can impair the quality of services provided to clients. The majority of research on compassion fatigue has focused on individual-level variables such as gender, history of trauma and age, among others. It is also imperative to understand the role played by alterable work-related characteristics in contributing to the development of compassion fatigue in order to attenuate its impact on mental health professionals and their clients. The present review examined articles exploring work-related factors associated with compassion fatigue. Fifteen quantitative studies were included and their quality was assessed using a checklist. An inductive content-analysis approach was adopted to synthesise the themes emerging from the data. The results suggested a theoretical model consistent with the Job Demands-Resources model, wherein job demands (such as workplace trauma, workload and therapeutic settings) are associated with compassion fatigue, and job resources (such as supervisors’, coworkers’ and organisational support) mitigate the impact of job demands. In addition to person-oriented factors, work-related factors are critical for the prevention of compassion fatigue.
Effect of Different Forms of Selenium on the Physiological Response and the Cadmium Uptake by Rice under Cadmium Stress
Authors: Xu Haizhao , Yan Jinpeng , Qin Yan , Xu Jingmao , Shohag M. J. I. , Wei Yanyan , Gu Minghua
Published: September 2020

Cadmium (Cd) is a pollutant toxic to plants and a potential threat to human health. Selenium (Se), though not essential for plants, has beneficial effects on plants under abiotic stress. A hydroponic experiment was conducted to investigate the impact of different forms of Se (Nano-Se, selenite, selenate, and SeMet) on accumulation, subcellular distribution, and chemical forms of Cd, as well as oxidative stress in rice seedlings. Cd (20 μmol·L−1) treatment significantly decreased biomass accumulation and chlorophyll content. The application of all Se forms, except selenate, mitigated the adverse effects of Cd on growth and chlorophyll content. The application of selenite, Nano-Se, and SeMet decreased root and shoot Cd concentrations as well as root-to-shoot Cd translocation in rice seedlings. Selenate application decreased shoot Cd concentration and root-to-shoot Cd translocation with no effect on root Cd concentration. Accordingly, Se increased the sequestration of Cd in the cell wall and vacuoles and decreased the active chemical form of Cd in rice seedlings. SeMet was the most effective supplement that decreased Cd concentration and enhanced Se concentration in the roots and shoots of rice seedlings. All forms of Se further enhanced catalase (CAT) and glutathione peroxidase (GSH-Px) activities and inhibited MDA accumulation. To conclude, Se influenced Cd accumulation and translocation in rice seedlings by altering the subcellular distribution, chemical forms, and antioxidant defense system under Cd stress. These effects were highly significant with SeMet treatment, probably due to better absorption and utilization by the plant.
Treg Enhancing Therapies to Treat Autoimmune Diseases
Authors: Eggenhuizen Peter J. , Ng Boaz H. , Ooi Joshua D.
Published: September 2020

Regulatory T cells (Tregs) are a small yet critical subset of CD4+ T cells, which have the role of maintaining immune homeostasis by, for example, regulating self-tolerance, tumor immunity, anti-microbial resistance, allergy and transplantation rejection. The suppressive mechanisms by which Tregs function are varied and pleiotropic. The ability of Tregs to maintain self-tolerance means they are critical for the control and prevention of autoimmune diseases. Irregularities in Treg function and number can result in loss of tolerance and autoimmune disease. Restoring immune homeostasis and tolerance through the promotion, activation or delivery of Tregs has emerged as a focus for therapies aimed at curing or controlling autoimmune diseases. Such therapies have focused on the Treg cell subset by using drugs to suppress T effector cells and promote Tregs. Other approaches have trialed inducing tolerance by administering the autoantigen via direct administration, by transient expression using a DNA vector, or by antigen-specific nanoparticles. More recently, cell-based therapies have been developed as an approach to directly or indirectly enhance Treg cell specificity, function and number. This can be achieved indirectly by transfer of tolerogenic dendritic cells, which have the potential to expand antigen-specific Treg cells. Treg cells can be directly administered to treat autoimmune disease by way of polyclonal Tregs or Tregs transduced with a receptor with high affinity for the target autoantigen, such as a high affinity T cell receptor (TCR) or a chimeric antigen receptor (CAR). This review will discuss the strategies being developed to redirect autoimmune responses to a state of immune tolerance, with the aim of the prevention or amelioration of autoimmune disease.
Macular Ganglion Cell Complex and Peripapillary Retinal Nerve Fiber Layer Thinning in Patients with Type-1 Gaucher Disease
Authors: Weill Yishay , Zimran Ari , Zadok David , Wasser Lauren M. , Revel-Vilk Shoshana , Hanhart Joel , Dinur Tama , Arkadir David , Becker-Cohen Michal
Published: September 2020

Type-1 Gaucher disease (GD1) is considered to be non- neuronopathic however recent evidence of neurological involvement continues to accumulate. There is limited evidence of retinal abnormalities in GD1. The purpose of this study was to evaluate the retinal findings of patients with GD1. Thirty GD1 individuals and 30 healthy volunteers between the ages 40–75 years were prospectively enrolled. Macular and optic nerve optical coherence tomography (OCT) scans of both eyes of each patient were performed and thickness maps were compared between groups. Patients with a known neurodegenerative disease, glaucoma, high myopia and previous intraocular surgeries were excluded. It was shown that patients with GD1 presented with higher incidence of abnormal pRNFL OCT scan and showed significantly thinner areas of pRNFL and macular ganglion cell complex (GCC) when compared to a healthy control population. Changes in retinal thickness were not associated with GD1 genotype, treatment status, disease monitoring biomarker (lyso-Gb1) and severity score index (Zimran SSI). Further investigations are needed to determine whether these findings possess functional visual implications and if retinal thinning may serve as biomarker for the development of future neurodegenerative disease in this population.
Immune Escape Mechanisms and Their Clinical Relevance in Head and Neck Squamous Cell Carcinoma
Authors: Seliger Barbara , Massa Chiara , Yang Bo , Bethmann Daniel , Kappler Matthias , Eckert Alexander Walter , Wickenhauser Claudia
Published: September 2020

Immunotherapy has been recently approved for the treatment of relapsed and metastatic human papilloma virus (HPV) positive and negative head and neck squamous cell carcinoma (HNSCC). However, the response of patients is limited and the overall survival remains short with a low rate of long-term survivors. There exists growing evidence that complex and partially redundant immune escape mechanisms play an important role for the low efficacy of immunotherapies in this disease. These are caused by diverse complex processes characterized by (i) changes in the expression of immune modulatory molecules in tumor cells, (ii) alterations in the frequency, composition and clonal expansion of immune cell subpopulations in the tumor microenvironment and peripheral blood leading to reduced innate and adaptive immune responses, (iii) impaired homing of immune cells to the tumor site as well as (iv) the presence of immune suppressive soluble and physical factors in the tumor microenvironment. We here summarize the major immune escape strategies of HNSCC lesions, highlight pathways, and molecular targets that help to attenuate HNSCC-induced immune tolerance, affect the selection and success of immunotherapeutic approaches to overcome resistance to immunotherapy by targeting immune escape mechanisms and thus improve the HNSCC patients’ outcome.