bluuebunny/crossref_metadata_2025 huggingface dataset:
The boostless bootstrap: amplitudes without Lorentz boosts
Authors: Pajer Enrico , Stefanyszyn David , Supeł Jakub
Published: January 2021

Abstract

Poincaré invariance is a well-tested symmetry of nature and sits at the core of our description of relativistic particles and gravity. At the same time, in most systems Poincaré invariance is not a symmetry of the ground state and is hence broken spontaneously. This phenomenon is ubiquitous in cosmology where Lorentz boosts are spontaneously broken by the existence of a preferred reference frame in which the universe is homogeneous and isotropic. This motivates us to study scattering amplitudes without requiring invariance of the interactions under Lorentz boosts. In particular, using on-shell methods and assuming massless, relativistic and luminal particles of any spin, we show that the allowed interactions around Minkowski spacetime are severely constrained by unitarity and locality in the form of consistent factorization. The existence of an interacting massless spin-2 particle enforces (analytically continued) three-particle amplitudes to be Lorentz invariant, even those that do not involve a graviton, such as cubic scalar couplings. We conjecture this to be true for all n-particle amplitudes. Also, particles of spin S > 2 cannot self-interact nor can be minimally coupled to gravity, while particles of spin S > 1 cannot have electric charge. Given the growing evidence that free gravitons are well described by massless, luminal relativistic particles, our results imply that cubic graviton interactions in Minkowski must be those of general relativity up to a unique Lorentz-invariant higher-derivative correction of mass dimension 9. Finally, we point out that consistent factorization for massless particles is highly IR sensitive and therefore our powerful flat-space results do not straightforwardly apply to curved spacetime.
Contribution of the Darwin operator to non-leptonic decays of heavy quarks
Authors: Lenz Alexander , Piscopo Maria Laura , Rusov Aleksey V.
Published: January 2021

Abstract

We compute the Darwin operator contribution ($$ 1/{m}_b^3 $$
1
/

m
b
3

correction) to the width of the inclusive non-leptonic decay of a B meson (B+, Bd or Bs), stemming from the quark flavour-changing transition b → $$ {q}_1{\overline{q}}_2{q}_3 $$

q
1

q
¯

2

q
3

, where q1, q2 = u, c and q3 = d, s. The key ideas of the computation are the local expansion of the quark propagator in the external gluon field including terms with a covariant derivative of the gluon field strength tensor and the standard technique of the Heavy Quark Expansion (HQE). We confirm the previously known expressions of the $$ 1/{m}_b^3 $$
1
/

m
b
3

contributions to the semi-leptonic decay b → $$ {q}_1\mathrm{\ell}{\overline{\nu}}_{\mathrm{\ell}} $$

q
1

ℓ

ν
¯

ℓ

, with ℓ = e, μ, τ and of the $$ 1/{m}_b^2 $$
1
/

m
b
2

contributions to the non-leptonic modes. We find that this new term can give a sizeable correction of about −4 % to the non-leptonic decay width of a B meson. For Bd and Bs mesons this turns out to be the dominant correction to the free b-quark decay, while for the B+ meson the Darwin term gives the second most important correction — roughly 1/2 to 1/3 of the phase space enhanced Pauli interference contribution. Due to the tiny experimental uncertainties in lifetime measurements the incorporation of the Darwin term contribution is crucial for precision tests of the Standard Model.
Large p explorations. From SUGRA to big STRINGS in Mellin space
Authors: Aprile Francesco , Vieira Pedro
Published: January 2021

Abstract

We explore a new way of probing scattering of closed strings in AdS5×S5, which we call ‘the large p limit’. It consists of studying four-point correlators of single-particle operators in $$ \mathcal{N} $$
N
= 4 SYM at large N and large ’t Hooft coupling λ, by looking at the regime in which the dual KK modes become short massive strings. In this regime the charge of the single-particle operators is order λ1/4 and the dual KK modes are in between fields and strings. Starting from SUGRA we compute the large p limit of the correlators by introducing an improved AdS5×S5 Mellin space amplitude, and we show that the correlator is dominated by a saddle point. Our results are consistent with the picture of four geodesics shooting from the boundary of AdS5×S5 towards a common bulk point, where they scatter as if they were in flat space. The Mandelstam invariants are put in correspondence with the Mellin variables and in turn with certain combinations of cross ratios. At the saddle point the dynamics of the correlator is directly related to the bulk Mellin amplitude, which in the process of taking large p becomes the flat space ten-dimensional S-matrix. We thus learn how to embed the full type IIB S-matrix in the AdS5×S5 Mellin amplitude, and how to stratify the latter in a large p expansion. We compute the large p limit of all genus zero data currently available, pointing out additional hidden simplicity of known results. We then show that the genus zero resummation at large p naturally leads to the Gross-Mende phase for the minimal area surface around the bulk point. At one-loop, we first uncover a novel and finite Mellin amplitude, and then we show that the large p limit beautifully asymptotes the gravitational S-matrix.
OsPPR939, a nad5 splicing factor, is essential for plant growth and pollen development in rice
Authors: Zheng Peng , Liu Yujun , Liu Xuejiao , Huang Yuqing , Sun Feng , Wang Wenyi , Chen Hao , Jan Mehmood , Zhang Cuicui , Yuan Yue , Tan Bao-Cai , Du Hao , Tu Jumin
Published: January 2021

Key message

P-subfamily PPR protein OsPPR939, which can be phosphorylated by OsS6K1, regulates plant growth and pollen development by involving in the splicing of mitochondrialnad5introns 1, 2, and 3.

In land plants, pentatricopeptide repeat (PPR) proteins play key roles in mitochondrial group II intron splicing, but how these nucleus-encoded proteins are imported into mitochondria is unknown. To date, a few PPR proteins have been characterized in rice (Oryza sativa). Here, we demonstrate that the mitochondrion-localized P-subfamily PPR protein OsPPR939 is required for the splicing ofnad5introns 1, 2, and 3 in rice. Complete knockout or partial disruption of OsPPR939 function resulted in different degrees of growth retardation and pollen sterility. The dramatically reduced splicing efficiency of these introns inosppr939-4andosppr939-5led to reduced mitochondrial complex I abundance and activity and enhanced expression of alternative respiratory pathway genes. Complementation withOsPPR939rescued the defective plant morphology ofosppr939-4and restored its decreased splicing efficiency ofnad5introns 1, 2, and 3. Therefore, OsPPR939 plays crucial roles in plant growth and pollen development by splicing mitochondrialnad5introns 1, 2, and 3. More importantly, the 12th amino acid Ser in the N-terminal targeting sequence of OsPPR939 is phosphorylated by OsS6K1, and truncated OsPPR939 with a non-phosphorylatable S12A mutation in its presequence could not be imported into mitochondria, suggesting that phosphorylation of this amino acid plays an important role in the mitochondrial import of OsPPR939. To our knowledge, the 12th residue Ser on OsPPR939 is the first experimentally proven phosphorylation site in PPR proteins. Our results provide a basis for investigating the regulatory mechanism of PPR proteins at the post-translational level.
Alien introgression and morpho-agronomic characterization of diploid progenies of Solanum lycopersicoides monosomic alien addition lines (MAALs) toward pre-breeding applications in tomato (S. lycopersicum)
Authors: Mangat Puneet Kaur , Shim Junghyun , Gannaban Ritchel B. , Singleton Joshua J. , Angeles-Shim Rosalyn B.
Published: January 2021

Key message

Alien introgressions that were captured in the genome of diploid plants segregating from progenies of monosomic alien addition lines ofS. lycopersicoidesconfer novel phenotypes with commercial and agronomic value in tomato breeding.

Solanum lycopersicoidesis a wild relative of tomato with a natural adaptation to a wide array of biotic and abiotic challenges. In this study, we identified and characterized diploid plants segregating from the progenies of monosomic alien addition lines (MAALs) ofS. lycopersicoidesto establish their potential as donors in breeding for target trait improvement in tomato. Molecular genotyping identified 28 of 38 MAAL progenies having the complete chromosome complement of the cultivated tomato parent and limited chromosome introgressions from the wildS. lycopersicoidesparent. Analysis of SSR and indel marker profiles identified 34 unique alien introgressions in the 28 MAAL-derived introgression lines (MDILs) in the genetic background of tomato. Conserved patterns of alien introgressions were detected among sibs of MDILs 2, 3, 4 and 8. Across MDILs, a degree of preferential transmission of specific chromosome segments was also observed. Morphologically, the MDILs closely resembled the cultivated tomato more thanS. lycopersicoides. The appearance of novel phenotypes in the MDILs that are lacking in the cultivated parent or the source MAALs indicates the capture of novel genetic variation by the diploid introgression lines that can add commercial and agronomic value to tomato. In particular, screening of representative MDILs for drought tolerance at the vegetative stage identified MDIL 2 and MDIL 11III as drought tolerant based on visual scoring. A regulated increase in stomatal conductance of MDIL 2 under drought stress indicates better water use efficiency that allowed it to survive for 7 days under 0% moisture level.
Over-constrained kinematic of the medial compartment leads to lower clinical outcomes after total knee arthroplasty
Authors: Pizza Nicola , Di Paolo Stefano , Zinno Raffaele , Marcheggiani Muccioli Giulio Maria , Agostinone Piero , Alesi Domenico , Bontempi Marco , Zaffagnini Stefano , Bragonzoni Laura
Published: January 2021

Purpose

To investigate if postoperative clinical outcomes correlate with specific kinematic patterns after total knee arthroplasty (TKA) surgery. The hypothesis was that the group of patients with higher clinical outcomes would have shown postoperative medial pivot kinematics, while the group of patients with lower clinical outcomes would have not.

Methods

52 patients undergoing TKA surgery were prospectively evaluated at least a year of follow-up (13.5 ± 6.8 months) through clinical and functional Knee Society Score (KSS), and kinematically through dynamic radiostereometric analysis (RSA) during a sit-to-stand motor task. Patients received posterior-stabilized TKA design. Based on the result of the KSS, patients were divided into two groups: “KSS > 70 group”, patients with a good-to-excellent score (93.1 ± 6.8 points, n = 44); “KSS < 70 group”, patients with a fair-to-poor score (53.3 ± 18.3 points, n = 8). The anteroposterior (AP) low point (lowest femorotibial contact points) translation of medial and lateral femoral compartments was compared through Student’s t test (p < 0.05).

Results

Low point AP translation of the medial compartment was significantly lower (p < 0.05) than the lateral one in both the KSS > 70 (6.1 mm ± 4.4 mm vs 10.7 mm ± 4.6 mm) and the KSS < 70 groups (2.7 mm ± 3.5 mm vs 11.0 mm ± 5.6 mm). Furthermore, the AP translation of the lateral femoral compartment was not significantly different (p > 0.05) between the two groups, while the AP translation of the medial femoral compartment was significantly higher for the KSS > 70 group (p = 0.0442).

Conclusion

In the group of patients with a postoperative KSS < 70, the medial compartment translation was almost one-fourth of the lateral one. Surgeons should be aware that an over-constrained kinematic of the medial compartment might lead to lower clinical outcomes.

Level of evidence

II.
The effects of locations on the build tray on the quality of specimens in powder bed additive manufacturing
Authors: Pal Snehashis , Gubeljak Nenad , Bončina Tonica , Hudák Radovan , Toth Teodor , Zivcak Jozef , Lojen Gorazd , Leben Niko , Drstvenšek Igor
Published: January 2021

In this study, the effect of powder spreading direction was investigated on selectively laser-melted specimens. The results showed that the metallurgical properties of the specimens varied during fabrication with respect to their position on the build tray. The density, porosity, and tensile properties of the Co–Cr–W–Mo alloy were investigated on cuboid and tensile specimens fabricated at different locations. Two different significant positions on the tray were selected along the powder spreading direction. One set of specimens was located near the start line of powder spreading, and the other set was located near the end of the building tray. The main role in the consequences of powder layering was played by the distribution of powder particle sizes and the packing density of the layers. As a result, laser penetration, melt pool formation, and fusion characteristics varied. To confirm the occurrence of variations in sample density, an additional experiment was performed with a Ti–6Al–4V alloy. Furthermore, the powders were collected at two different fabricating locations and their size distribution for both materials was investigated.
Effects of holding soft objects during Cyberball tasks under frequent positive feedback
Authors: Ikeda Toshiki , Takeda Yuji
Published: January 2021

A previous study suggested that holding soft objects enhanced expectations of uncertain events and increased social pain under frequent negative feedback; i.e., higher expectations might have induced more disappointment. The present study examined the effects of holding a soft cushion under frequent positive feedback. Participants (n = 42) performed fair-play and over-inclusion blocks in the Cyberball task. Amplitudes of the contingent negative variation (CNV) of event-related brain potentials and subjective ratings of social pain were measured to estimate participants’ expectations and emotions, respectively. CNV amplitudes were higher in the over-inclusion block when participants held the soft than the hard cushion. There was a statistically marginal trend (p = .095) for lower social pain scores in the soft cushion condition than the hard cushion condition in contrast to previous findings. These results suggest that holding a soft object does not directly modulate emotions but instead acts through the mediation of enhanced expectations.
The influence of recipient SLCO1B1 rs2291075 polymorphism on tacrolimus dose–corrected trough concentration in the early period after liver transplantation
Authors: Wu Yi , Fang Fang , Wang Zhaowen , Wen Peihao , Fan Junwei
Published: January 2021

Purpose

To explore the relationship between rs2291075 polymorphism in SLCO1B1 gene, which encodes an influx transmembrane protein transporter, and tacrolimus dose–corrected trough concentration (C/D, ng ml−1 mg−1 kg−1) in the early period after liver transplantation.

Methods

CYP3A5 rs776746 and SLCO1B1 rs2291075 polymorphisms of 210 liver transplantation patients and their corresponding donor livers were assessed by PCR amplification and DNA sequencing. The influence of gene polymorphisms on C/D values of tacrolimus was analyzed. The early postoperative period after liver transplantation was divided into the convalescence phase (1–14 days) and stationary phase (15–28 days) according to the change of liver function and tacrolimus C/D values.

Results

The combined analysis of donor and recipient CYP3A5 rs776746 could distinguish the metabolic phenotype of tacrolimus into three groups: fast elimination (FE), intermediate elimination (IE), and slow elimination (SE), which was entitled the FIS classification system. Tacrolimus C/D ratios of recipient SLCO1B1 rs2291075 CT and TT carriers were very close and were significantly lower than those of recipient SLCO1B1 rs2291075 CC genotype carriers in convalescence phase (p = 0.0195) and in stationary phase (p = 0.0152). There were no statistically significant differences between tacrolimus C/D ratios of patients carried with SLCO1B1 rs2291075 CT, TT genotype donors, and those carried with SLCO1B1 rs2291075 CC genotype donors. A model consisting of tacrolimus daily dose, total bilirubin, FIS classification, and recipient SLCO1B1 rs2291075 could predict tacrolimus C/D ratios in the convalescence phase by multivariate analysis. However, recipient SLCO1B1 rs2291075 genotype failed to enter forecast model for C/D ratios in stationary phase. Recipient SLCO1B1 rs2291075 genotype had significant effect on tacrolimus C/D ratios in convalescence phase (p = 0.0300) and stationary phase (p = 0.0400) in subgroup, which excluded the interference come from donor and recipient CYP3A5 rs776746.

Conclusion

SLCO1B1 rs2291075 could be a novel genetic locus associated with tacrolimus metabolism. The combined analysis of donor and recipient CYP3A5 rs776746, recipient SLCO1B1 rs2291075 genotypes, could be helpful to guide the personalized administration of tacrolimus in early period after liver transplantation.
Immune signature-based risk stratification and prediction of immune checkpoint inhibitor’s efficacy for lung adenocarcinoma
Authors: Yi Ming , Li Anping , Zhou Linghui , Chu Qian , Luo Suxia , Wu Kongming
Published: January 2021

Background

Lung adenocarcinoma (LUAD) is a common pulmonary malignant disease with a poor prognosis. There were limited studies investigating the influences of the tumor immune microenvironment on LUAD patients’ survival and response to immune checkpoint inhibitors (ICIs).

Methods

Based on TCGA-LUAD dataset, we constructed a prognostic immune signature and validated its predictive capability in the internal as well as total datasets. Then, we explored the differences of tumor-infiltrating lymphocytes, tumor mutation burden, and patients’ response to ICI treatment between the high-risk score group and low-risk score group.

Results

This immune signature consisted of 17 immune-related genes, which was an independent prognostic factor for LUAD patients. In the low-risk score group, patients had better overall survival. Although the differences were non-significant, patients with low-risk scores had more tumor-infiltrating follicular helper T cells and fewer macrophages (M0), which were closely related to clinical outcomes. Additionally, the total TMB was markedly decreased in the low-risk score group. Using immunophenoscore as a surrogate of ICI response, we found that patients with low-risk scores had significantly higher immunophenoscore.

Conclusion

The 17-immune-related genes signature may have prognostic and predictive relevance with ICI therapy but needs prospective validation.
Why compromise the patella? Five-year follow-up results of medial patellofemoral ligament reconstruction with soft tissue patellar fixation
Authors: Boelch Sebastian P. , Gurok Anna , Gilbert Fabian , Weißenberger Manuel , Rudert Maximilian , Barthel Thomas , Reppenhagen Stephan
Published: January 2021

Purpose

This study investigates the redislocation rate and functional outcome at a minimum follow-up of five years after medial patellofemoral ligament (MPFL) reconstruction with soft tissue patellar fixation for patella instability.

Methods

Patients were retrospectively identified and knees were evaluated for trochlea dysplasia according to Dejour, for presence of patella alta and for presence of cartilage lesion at surgery. At a minimum follow-up of five years, information about an incident of redislocation was obtained. Kujala, Lysholm, and Tegner questionnaires as well as range of motion were used to measure functional outcome.

Results

Eighty-nine knees were included. Follow-up rate for redislocation was 79.8% and for functional outcome 58.4%. After a mean follow-up of 5.8 years, the redislocation rate was 5.6%. There was significant improvement of the Kujala score (68.8 to 88.2, p = 0.000) and of the Lysholm score (71.3 to 88.4, p = 0.000). Range of motion at follow-up was 149.0° (115–165). 77.5% of the knees had patella alta and 52.9% trochlear dysplasia types B, C, or D. Patellar cartilage legions were present in 54.2%. Redislocations occurred in knees with trochlear dysplasia type C in combination with patella alta.

Conclusion

MPFL reconstruction with soft tissue patellar fixation leads to significant improvement of knee function and low midterm redislocation rate. Patients with high-grade trochlear dysplasia should be considered for additional osseous correction.
Influence of Northern Hemispheric Winter Warming on the Pacific Storm Track
Authors: Park Hyung-Ju , Kim Kwang-Yul
Published: January 2021

Effect of global warming on the sub-seasonal variability of the Northern Hemispheric winter (NDJFM) Pacific storm-track (PST) activity has been investigated. Previous studies showed that the winter-averaged PST has shifted northward and intensified, which was explained in terms of energy exchange with the mean field. Effect of global warming exhibits spatio-temporal heterogeneity with predominance over the Arctic region and in the winter season. Therefore, seasonal averaging may hide important features on sub-seasonal scales. In this study, distinct sub-seasonal response in storm track activities to winter Northern Hemispheric warming is analyzed applying cyclostationary empirical orthogonal function analysis to ERA5 data. The key findings are as follows. Change in the PST is not uniform throughout the winter; the PST shifts northward in early winter (NDJ) and intensifies in late winter (FM). In early winter, the combined effect of weakened baroclinic process to the south of the climatological PST and weakened barotropic damping to the north is responsible for the northward shift. In late winter, both processes contribute to the amplification of the PST. Further, change in baroclinic energy conversion is quantitatively dominated by eddy heat flux, whereas axial tilting of eddies is primarily responsible for change in barotropic energy conversion. A close relationship between anomalous eddy heat flux and anomalous boundary heating, which is largely determined by surface turbulent heat flux, is also demonstrated.
The differing role of weather systems in southern Australian rainfall between 1979–1996 and 1997–2015
Authors: Pepler Acacia S. , Dowdy Andrew J. , Hope Pandora
Published: January 2021

Most of the rainfall in southern Australia is associated with cyclones, cold fronts, and thunderstorms, and cases when these weather systems co-occur are particularly likely to cause extreme rainfall. Rainfall declines in some parts of southern Australia during the cool half of the year in recent decades have previously been attributed to decreases in the rainfall from fronts and/or cyclones, while thunderstorm-related rainfall has been observed to increase, particularly in the warm half of the year. However, the co-occurrence of these systems, particularly the co-occurrence of cyclones or fronts with thunderstorms, can be very important for rainfall in some areas, particularly heavy rainfall, and changes in the frequency of these combined weather systems have not been previously assessed. In this paper we show that the majority of the observed cool season rainfall decline between 1979–1996 and 1997–2015 in southeast Australia is associated with a decrease in the frequency of fronts and cyclones that produce rainfall, while there has simultaneously been an increase in the frequency of cold fronts and thunderstorms that do not produce rainfall in some regions. Thunderstorm rainfall has increased in much of southern Australia, particularly during the warm half of the year, including an increase in rainfall where a thunderstorm environment occurs at the same time as a cyclone or front.
Telemedicine in the pediatric surgery in Germany during the COVID-19 pandemic
Authors: Lakshin G. , Banek S. , Keese D. , Rolle U. , Schmedding A.
Published: January 2021

Purpose

The COVID-19 pandemic has led to an unprecedented expansion of telemedicine services worldwide. This study aimed to explore the practice of telemedicine in Pediatric Surgery in Germany, the impact of the pandemic on its development and parents’ and surgeons’ experiences with telemedicine.

Methods

The study is a cross-sectional analysis using three surveys between 6/2020 and 10/2020: (1) all Pediatric Surgery departments of Germany reported whether they provide telemedicine services. (2) Members of the German Society of Pediatric Surgery and (3) families who participated in an outpatient visit by telephone or video with the Department of Pediatric Surgery and Pediatric Urology of the University Hospital Frankfurt completed an anonymous survey on their experience with telemedicine.

Results

21% of the Pediatric Surgery departments in Germany provided telemedicine, of which 57% started due to the pandemic. The lack of physical examination and face-to-face contact seem to be the major limitations to surgeons and parents. 48% of the parents answered that telemedicine is equal to or better than traditional appointments, while 33% thought that telemedicine is worse.

Conclusions

This study shows that families and doctors alike have had positive experiences with telemedicine and most will continue to use this format after the pandemic.
Scientometric evaluation of highly cited scientists in the field of forensic science and legal medicine
Authors: Jones Alan Wayne
Published: January 2021

A publically available database of the most highly cited scientists in all disciplines was used to identify people that belonged to the subject category “forensic science and legal medicine.” This bibliometric information was derived from Elsevier’s SCOPUS database containing eight million scientists with at least five articles as author or co-author. The top 100,000 most highly cited scientists were identified and ranked according to six citation metrics; total number of citations, H-index, H-index adjusted for co-authorship, citations to single-authored papers, citations to single or first author papers and, citations to single, first, or last-authored papers. The eight million entries in the SCOPUS database were sub-divided into 22 main subject categories and 176 sub-categories, one of which was legal and forensic medicine. The citation databases were provided as supplementary material in two articles published in PLoS Biology in 2019 and 2020. Among the top 100,000 most highly cited scientists, there were only 30 allocated to the legal and forensic medicine category, according to the 2019 PLoS Biology article. The updated database from 2020 also included the names of people within the top-cited 2% of their scientific discipline. This increased the number of forensic practitioners to 215 from a total of 10,158 individuals in this subject category. This article takes a closer look at these highly cited forensic scientists, the countries where they work, the particular research field in which they publish, and their composite citation scores with and without self-citations. The top ten most cited individuals in both databases (2019 and 2020) were the same and these should therefore be considered an elite group among all forensic practitioners.
Soluble αKlotho downregulates Orai1-mediated store-operated Ca2+ entry via PI3K-dependent signaling
Authors: Kim Ji-Hee , Park Eun Young , Hwang Kyu-Hee , Park Kyu-Sang , Choi Seong Jin , Cha Seung-Kuy
Published: January 2021

αKlotho is a type 1 transmembrane anti-aging protein. αKlotho-deficient mice have premature aging phenotypes and an imbalance of ion homeostasis including Ca2+ and phosphate. Soluble αKlotho is known to regulate multiple ion channels and growth factor-mediated phosphoinositide-3-kinase (PI3K) signaling. Store-operated Ca2+ entry (SOCE) mediated by pore-forming subunit Orai1 and ER Ca2+ sensor STIM1 is a ubiquitous Ca2+ influx mechanism and has been implicated in multiple diseases. However, it is currently unknown whether soluble αKlotho regulates Orai1-mediated SOCE via PI3K-dependent signaling. Among the Klotho family, αKlotho downregulates SOCE while βKlotho or γKlotho does not affect SOCE. Soluble αKlotho suppresses serum-stimulated SOCE and Ca2+ release-activated Ca2+ (CRAC) channel currents. Serum increases the cell-surface abundance of Orai1 via stimulating vesicular exocytosis of the channel. The serum-stimulated SOCE and cell-surface abundance of Orai1 are inhibited by the preincubation of αKlotho protein or PI3K inhibitors. Moreover, the inhibition of SOCE and cell-surface abundance of Orai1 by pretreatment of brefeldin A or tetanus toxin or PI3K inhibitors prevents further inhibition by αKlotho. Functionally, we further show that soluble αKlotho ameliorates serum-stimulated SOCE and cell migration in breast and lung cancer cells. These results demonstrate that soluble αKlotho downregulates SOCE by inhibiting PI3K-driven vesicular exocytosis of the Orai1 channel and contributes to the suppression of SOCE-mediated tumor cell migration.
The role of clinical factors and immunocheckpoint molecules in the prognosis of patients with supratentorial extraventricular ependymoma: a single-center retrospective study
Authors: Wang Liguo , Han Song , Yan Changxiang , Yang Yakun , Li Zhiqiang , Yang Zuocheng
Published: January 2021

Purpose

Supratentorial extraventricular ependymoma (SEE) is a rare subset of ependymomas located in the supratentorial parenchyma, and little is known regarding its management and prognosis. Our study aimed to reveal the prognostic factors in patients with SEE and the roles of programmed death ligand-1 (PD-L1), programmed cell death protein 1 (PD-1), Ki-67, and neural cell adhesion molecule L1 (L1CAM) in predicting these patients’ outcomes.

Methods

We retrospectively studied the clinical features and prognostic factors in 48 patients with SEE admitted to our center from April 2008 to October 2018. Tissue slides were constructed from patient samples, and PD-L1, PD-1, Ki-67, and L1CAM expression levels were evaluated by immunohistochemistry.

Results

Patients with gross total resection (GTR) had better progression-free survival than patients with subtotal resection (STR). Moreover, the recurrence hazard ratios in patients with STR at 3, 5, and 10 years were 8.746, 6.866 and 3.962 times those of patients with GTR, respectively. PD-L1 positivity predicted worse progression-free survival, while the recurrence hazard ratios for patients with PD-L1 positivity at 3, 5, and 10 years were 10.445, 5.539, and 3.949 times those of patients with PD-L1 negativity, respectively. Multivariate analysis revealed that PD-L1 expression and GTR could independently predict outcomes in patients with SEE.

Conclusion

PD-L1 expression was an independent and more readily obtained predictor of outcomes, representing a simple and reliable biological prognostic factor for patients with SEE. Further studies are needed to explore PD-L1 inhibitor treatment for patients with ependymoma.

Clinical trial registration

No clinical trials were performed in the study.
Lyme neuroborreliosis in Swedish children—PCR as a complementary diagnostic method for detection of Borrelia burgdorferi sensu lato in cerebrospinal fluid
Authors: Skogman Barbro H. , Wilhelmsson Peter , Atallah Stephanie , Petersson Ann-Cathrine , Ornstein Katarina , Lindgren Per-Eric
Published: January 2021

The aim of this study was to evaluate polymerase chain reaction (PCR) as a diagnostic method for the detection of Borrelia burgdorferi s.l. in CSF of Swedish children with LNB. This study was performed retrospectively on CSF and serum samples collected from children evaluated for LNB (n = 233) and controls with other specific neurological disorders (n = 59) in a Swedish Lyme endemic area. For anti-Borrelia antibody index, the IDEIA Lyme Neuroborreliosis kit (Oxoid) was used. Two in-house real-time PCR assays targeting the 16S rRNA gene were evaluated (TaqMan® and LUX™). Among patients classified as LNB cases (n = 102), five children (5%) were Borrelia PCR-positive in CSF with the TaqMan® assay. In the Non-LNB group (n = 131), one patient was Borrelia PCR positive with the TaqMan® assay. Among controls (n = 59), all CSF samples were PCR negative. When amplifying and sequencing ospA, we found B. garinii (n = 2), B. afzelii (n = 2), B. bavariensis (n = 1), and one untypable (n = 1). With the LUX™ technology, all CSF samples were PCR negative. The TaqMan® assay could detect only few cases (n = 6) of B. burgdorferi s.l. in CSF among children with LNB and the sensitivity was very low (5%). However, using larger CSF volumes and centrifugation of samples, the PCR technique could still be useful as a complementary diagnostic method when evaluating LNB. Furthermore, detection of spirochete DNA in clinical matrices, including CSF, is the method of choice for studying epidemiological aspects of LNB, a tick-borne emerging disease.
Housing Instability and HIV Risk: Expanding our Understanding of the Impact of Eviction and Other Landlord-Related Forced Moves
Authors: Groves Allison K. , Niccolai Linda M. , Keene Danya E. , Rosenberg Alana , Schlesinger Penelope , Blankenship Kim M.
Published: January 2021

The study purpose is to comprehensively measure landlord-related forced moves (inclusive of, but not restricted to, legal eviction), and to examine whether landlord-related forced moves is associated with HIV risk. Baseline survey data was collected between 2017 and 2018 among 360 low-income participants in New Haven, Connecticut. We used multivariable logistic regression analyses to examine associations between landlord-related forced moves and HIV sexual risk outcomes. Seventy seven out of three hundred and sixty participants reported a landlord-related forced move in the past 2 years, of whom 19% reported formal eviction, 56% reported informal eviction and 25% reported both. Landlord-related forced moves were associated with higher odds of unprotected sex (AOR 1.98), concurrent sex (AOR 1.94), selling sex for money or drugs (AOR 3.28), exchange of sex for a place to live (AOR 3.29), and an HIV sexual risk composite (ARR 1.46) (p < .05 for all). We found robust associations between landlord-related forced moves and HIV sexual risk. Findings suggest that the social and economic consequences of landlord-related forced moves may impact sexual vulnerability.
A low-power 10-bit 0.01-to-12-MS/s asynchronous SAR ADC in 65-nm CMOS
Authors: Campos Arthur Lombardi , Navarro João , Luppe Maximiliam , de Lima Eduardo Rodrigues
Published: January 2021

During the last decades we have witnessed the performance improvement and the aggressive growth of the complexity of integrated circuits (ICs). The progressive size reduction of transistors in recent technological nodes has allowed and even compelled IC designers to perform analog tasks in the digital domain, increasing the demand for analog-to-digital converters (ADCs). This work presents the design and implementation of a low power, differential, asynchronous successive approximation register analog-to-digital converter (SAR ADC) in a 65-nm CMOS technology. The ADC works in a flexible range of sampling rates, from a few kS/s up to 12.0 MS/s, being suitable for application in a wide spectrum of low power systems and subsystems, such as biosignal recorder interfaces and frontend of wireless receivers. At maximum sampling rate, the post-layout simulated circuit achieved an effective number of bits (ENOB) of 9.65 and a power consumption of 151.4 µW, leading to a Figure of Merit of 15.8 fJ/Conversion-step; at 10.0 kS/s sampling rate, the ENOB is almost the same, 9.63, but the power consumption is reduced to only 0.26 µW. The occupied area of the implemented ADC is 0.074 mm2.
Description and genomic characterization of Streptococcus symci sp. nov., isolated from a child’s oropharynx
Authors: Qi He , Liu Defeng , Zou Yang , Wang Nan , Tian Han , Xiao Chunling
Published: January 2021

Using the culturomics approach, we isolated a new Streptococcus species, strain C17T, from the oropharynx mucosa sample of a healthy 5-year-old child living in Shenyang, China. We studied the phenotypic, phylogenetic, and genomic characteristics of strain C17T, which was identified as a Gram-positive, coccus-shaped, non-motile, aerobic, catalase-negative bacteria. Its growth temperatures ranged from 20 to 42 °C, with optimal growth at 37 °C. Acid production could be inhibited by two sugars, trehalose and raffinose. In C17T, the reactions for enzyme lipase (C14) were confirmed to be negative, whereas those for alkaline phosphatase, α-glucosidase, and hippuric acid hydrolysis were positive. The C17T genome contained 2,189,419 base pairs (bp), with an average G+C content of 39.95%, encoding 2092 genes in total. The 16S ribosomal RNA sequence showed 99.8% similarity with the newly identified Streptococcus pseudopneumoniae ATCC BAA-960T. The main fatty acid components in C17T were C16:0, C18:1 w7c, C18:0, and C18:1 w9c, all of which can be found in other species of the Streptococcus genus. Strain C17T showed high susceptibility to clindamycin, linezolid, vancomycin, chloramphenicol, and cefepime, and moderate susceptibility to erythromycin. The obtained dDDH value between strain C17T and the closest species was 52.9%. In addition, the whole genome sequence of strain C17T had an 82.21–93.40% average nucleotide identity (ANI) with those strains of closely related Streptococcus species, indicating that the strain C17T was unique among all Streptococcus species. Based on these characteristics, we determine that C17T is a novel species, named Streptococcus symci sp. nov. (= GDMCC 1.1633 = JCM 33582).
Live blog summarization
Authors: Avinesh P. V. S. , Peyrard Maxime , Meyer Christian M.
Published: January 2021

Live blogs are an increasingly popular news format to cover breaking news and live events in online journalism. Online news websites around the world are using this medium to give their readers a minute by minute update on an event. Good summaries enhance the value of the live blogs for a reader, but are often not available. In this article, (a) we first define the task of summarizing a live blog, (b) study ways of automatically collecting corpora for live blog summarization, and (c) understand the complexity of the task by empirically evaluating well-known state-of-the-art unsupervised and supervised summarization systems on our new corpus. We show that live blog summarization poses new challenges in the field of news summarization, since frequency and positional signals cannot be used. We make our tools publicly available to reconstruct the corpus and to conduct our empirical experiments. This encourages the research community to build upon and replicate our results.
Polyhedral approximations of the semidefinite cone and their application
Authors: Wang Yuzhu , Tanaka Akihiro , Yoshise Akiko
Published: January 2021

We develop techniques to construct a series of sparse polyhedral approximations of the semidefinite cone. Motivated by the semidefinite (SD) bases proposed by Tanaka and Yoshise (Ann Oper Res 265:155–182, 2018),
we propose a simple expansion of SD bases so as to keep the sparsity of the matrices composing it. We prove that the polyhedral approximation using our expanded SD bases contains the set of all diagonally dominant matrices and is contained in the set of all scaled diagonally dominant matrices. We also prove that the set of all scaled diagonally dominant matrices can be expressed using an infinite number of expanded SD bases. We use our approximations as the initial approximation in cutting plane methods for solving a semidefinite relaxation of the maximum stable set problem. It is found that the proposed methods with expanded SD bases are significantly more efficient than methods using other existing approximations or solving semidefinite relaxation problems directly.
Whole genome characterization of strains belonging to the Ralstonia solanacearum species complex and in silico analysis of TaqMan assays for detection in this heterogenous species complex
Authors: Kurm Viola , Houwers Ilse , Coipan Claudia E. , Bonants Peter , Waalwijk Cees , van der Lee Theo , Brankovics Balázs , van der Wolf Jan
Published: January 2021

Identification and classification of members of theRalstonia solanacearumspecies complex (RSSC) is challenging due to the heterogeneity of this complex. Whole genome sequence data of 225 strains were used to classify strains based on average nucleotide identity (ANI) and multilocus sequence analysis (MLSA). Based on the ANI score (>95%), 191 out of 192(99.5%) RSSC strains could be grouped into the three speciesR. solanacearum, R. pseudosolanacearum, andR. syzygii, and into the four phylotypes within the RSSC (I,II, III, and IV).R. solanacearumphylotype II could be split in two groups (IIA and IIB), from which IIB clustered in three subgroups (IIBa, IIBb and IIBc). This division by ANI was in accordance with MLSA. The IIB subgroups found by ANI and MLSA also differed in the number of SNPs in the primer and probe sites of various assays. Anin-silicoanalysis of eight TaqMan and 11 conventional PCR assays was performed using the whole genome sequences. Based on this analysis several cases of potential false positives or false negatives can be expected upon the use of these assays for their intended target organisms. Two TaqMan assays and two PCR assays targeting the 16S rDNA sequence should be able to detect all phylotypes of the RSSC. We conclude that the increasing availability of whole genome sequences is not only useful for classification of strains, but also shows potential for selection and evaluation of clade specific nucleic acid-based amplification methods within the RSSC.
Brief Report: Impact of COVID-19 on Individuals with ASD and Their Caregivers: A Perspective from the SPARK Cohort
Authors: White L. Casey , Law J. Kiely , Daniels Amy M. , Toroney Jaimie , Vernoia Brianna , Xiao Sabrina ,   The SPARK Consortium, Feliciano Pamela , Chung Wendy K.
Published: January 2021

The impact of the 2019 coronavirus pandemic (COVID-19) in the United States is unprecedented, with unknown implications for the autism community. We surveyed 3502 parents/caregivers of individuals with an autism spectrum disorder (ASD) enrolled in Simons Powering Autism Research for Knowledge (SPARK) and found that most individuals with ASD experienced significant, ongoing disruptions to therapies. While some services were adapted to telehealth format, most participants were not receiving such services at follow-up, and those who were reported minimal benefit. Children under age five had the most severely disrupted services and lowest reported benefit of telehealth adaptation. Caregivers also reported worsening ASD symptoms and moderate family distress. Strategies to support the ASD community should be immediately developed and implemented.
Reliability and longevity of implantable defibrillators
Authors: Hauser Robert G. , Casey Susan A. , Gitter Christopher B. , Tang Chuen Y. , Abdelhadi Raed H. , Gornick Charles C. , Stanberry Larissa , Sengupta Jay D.
Published: January 2021

Purpose

We hypothesized that data in manufacturers’ product performance reports (PPRs) can provide clinically valuable ICD and cardiac resynchronization defibrillator (CRT-D) reliability and longevity information.

Methods

Data were obtained from 2019 PPRs. Kaplan-Meier (K-M) probabilities of freedom from malfunction, normal battery depletion (NBD), and NBD + malfunction were calculated for ICD and CRT-D pulse generators (PGs) with LiMnO2 or LiSVO/CFx batteries marketed in the USA from 2010 to 2019 and compared using the log-rank test. Malfunctions (MAL) included PGs that were found outside specifications.

Results

Study population included 1,149,803 ICD and CRT-D PGs: Abbott (ABT; 35.1%), Biotronik (BIO; 4.6%), Boston Scientific (BSC; 23.5%), and Medtronic (MDT; 36.9%). Significant differences in reliability (p < 0.001), defined by freedom from MAL, were found between manufacturers; the majority of 6808 MAL occurred in ABT devices (n = 4045; 59.4%), followed by BSC (n = 2384; 35.0%), MDT (n = 338;5.0%), and BIO (n = 41; 0.6%). Battery failure (n = 890; 57.9%) was the most common cause of MAL compromising therapy; analysis of unique ABT battery MAL–indicated problem appeared a year prior to advisory. Significant differences (p < 0.001) in battery longevity, as defined by freedom from NBD, were found between manufacturers. Overall performance (freedom from NBD + MAL) favored BSC for CRT-D PGs and MDT and BIO for ICDs. BSC subcutaneous ICD reliability was inferior to its transvenous ICD (p < 0.001).

Conclusion

PPRs contain valuable data that can be aggregated and analyzed to inform physicians. Differences in product reliability exist between manufacturers. Battery longevity has improved, but MAL have significantly impacted performance. PPR data may be useful for assessing product problems and new technology.
Augmented Ultrasonic Data for Machine Learning
Authors: Virkkunen Iikka , Koskinen Tuomas , Jessen-Juhler Oskari , Rinta-aho Jari
Published: January 2021

Flaw detection in non-destructive testing, especially for complex signals like ultrasonic data, has thus far relied heavily on the expertise and judgement of trained human inspectors. While automated systems have been used for a long time, these have mostly been limited to using simple decision automation, such as signal amplitude threshold. The recent advances in various machine learning algorithms have solved many similarly difficult classification problems, that have previously been considered intractable. For non-destructive testing, encouraging results have already been reported in the open literature, but the use of machine learning is still very limited in NDT applications in the field. Key issue hindering their use, is the limited availability of representative flawed data-sets to be used for training. In the present paper, we develop modern, deep convolutional network to detect flaws from phased-array ultrasonic data. We make extensive use of data augmentation to enhance the initially limited raw data and to aid learning. The data augmentation utilizes virtual flaws—a technique, that has successfully been used in training human inspectors and is soon to be used in nuclear inspection qualification. The results from the machine learning classifier are compared to human performance. We show, that using sophisticated data augmentation, modern deep learning networks can be trained to achieve human-level performance.