bluuebunny/crossref_metadata_2025 huggingface dataset:
INSTAGRAM ACCOUNT MANAGEMENT STRATEGY @ARIESSKULINER AS COMMUNICATION MEDIA IN PROMOTING CULLINARY IN PONOROGO
Authors: Salsabila Andan Ayu
Published: August 2023

Communication and information technology is increasingly sophisticated and growing rapidly. The internet is the media most widely used by the public to find everything they want to know or just for existence, one of which is social media. One of the most popular social media today is Instagram. Ponorogo Regency is one of the regencies in East Java which has a very fast culinary tour. Instagram is one of the media for promotion. Therefore, when using Instagram social media accounts, you must use a management strategy to fit the plan. This study aims to explain: (1) the strategy for managing the Instagram account @ariesskuliner in promoting culinary in Ponorgo, (2) the results of managing the Instagram account @ariesskuliner, (3) the challenges in managing Instagram @ariesskuliner. The research method used is a qualitative approach. Data collection techniques used are interviews, observation, and documentation. The data analysis used is the Miles and Huberman model. The informants in this study are the founders of the @arisskuliner account, culinary entrepreneurs, and followers. The results of this study indicate that: First, the strategy for managing the Instagram @ariesskuliner account is the planning, implementation, monitoring, evaluation stages. Second, the results obtained in managing the @ariesskuliner account are a significant increase in followers every month, and culinary entrepreneurs are very satisfied with the delivery of content that is presented in an interesting way. Third, the challenges in managing the @ariesskuliner account are finding different content, negative perceptions, and consistency in uploading content.
Resiliensi Pada Single Mother Pasca Kematian Pasangan
Authors: Masrikah Ani
Published: August 2023

Resiliensi adalah suatu usaha, kekuatan dan kemampuan seorang individu untuk tetap bertahan dan bangkit dalam menghadapi masalah berat yang menimbulkan tekanan dalam dirinya. Tujuan penelitian ini adalah untuk mengetahui faktor-faktor Resiliensi Single Mother Pasca Kematian Pasangan di Kecamatan Ngimbang Kabupaten Lamongan, untuk mengetahui proses Resiliensi Single Mother Pasca Kematian Pasangan di Kecamatan Ngimbang Kabupaten Lamongan, dan untuk mengetahui dampak psikologis kematian pasangan bagi single mother pasca kematian pasangan di Kecamatan Ngimbang Kabupaten Lamongan. Metode penelitian yang digunakan dalam penelitian ini adalah kualitatif dengan pendekatan fenomenologi. Kesimpulan dari penelitian ini adalah pertama, faktor resiliensi pada single mother pasca kematian pasangan single mother memiliki ketujuh faktor resiliensi dalam diri masing-masing yakni  emotion regulation, impulse control, optimism, causal analysis, self efficacy, reaching out, dan emphathy. Kedua, proses resiliensi single mother melalui 4 tahapan proses resiliensi  ada empat tahapan yakni mengalah (Succumbing), bertahan (Survival), pemulihan (Recovery) dan berkembang pesat (Thriving). Ketiga, dampak psikologis kematian pasangan bagi single mother timbulnya perasaan sedih, terpukul, tekanan batin, dan syok, terpukul, tekanan batin, dan perasaan yang berubah-ubah.
kondisi kematangan emosi remaja setelah perceraian orangtua
Authors: Putira Tria
Published: August 2023

Penelitian ini membahas tentang kematangan emosi pada remaja setelah perceraian orangtua. Tujuan dari penelitian ini yaitu untuk mengetahui kemampuan remaja mengendalikan emosi, kemampuan penyesuaian diri remaja dan kondisi kematangan emosi remaja setelah perceraian orang tua. Kematangan emosi remaja dapat diartikan sebagai kemampuan individu untuk mengadakan tanggapan-tanggapan emosi secara matang dan mampu mengontrol serta mengendalikan emosinya sehingga menunjukkan suatu kesiapan dalam bertindak. Peneliti juga menggunakan aspek-aspek kematangan emosi, yaitu: realitas, mengetahui mana yang harus didahulukan, mengetahui tujuan jangka panjang, menerima tanggung jawab dan menunaikan kewajiban, menerima kegagalan, hubungan emosional, dan bertahap dalam memberikan reaksi. Sebagai acuan dalam penelitian ini serta melihat faktor-faktor yang mempengaruhi kematangan emosi pada subyek penelitian. Penelitian ini melibatkan lima remaja sebagai partisipan yangorangtuanyabercerai. Penelitian ini menggunakan tehnik pengambilan data berupa wawancara terstruktur dan observasi. Pengecekan keabsahan data dalam metode penelitian kualitatif menggunakan triangulasi dengan membandingkan hasil pengamatan dan hasil wawancara. Hasil penelitian menunjukkan bahwa dua partisipan sudah cukup mampu dalam mengontrol emosinya, dan tiga dan masih sulit mengontrol emosinya karena belum bisa  menerima kondisi keluarga saat ini. Dua partisipan yang memiliki kematangan emosi yang sudah cukup matang  bisa menyesuaikan diri dengan lingkungan sekitar, dan tiga partisipan yang belum memiliki kematangan emosi belum mampu menyesuaikan diri dengan lingkungan. Dua partisipan sudah memiliki kematangan emosi yang cukup matang walaupun belum sepenuhnya, dan tiga partisipan belum meiliki kematangan emosi karena belum bisa menerima keadaan keluarganya setelah perceraian orangtua.
Pengaruh Penggunaan Multimedia Berbasis Android Terhadap Kemampuan Mengenal Bentuk Geometri Pada Anak Usia 5-6 Tahun
Authors: Masniah Masniah
Published: August 2023

Pada penelitian ini peneliti menggunakan teknologi multimedia berbasis android dengan menggunakan aplikasi mengenal bentuk geometri untuk anak usia 5-6 tahun. Penelitian ini menggunakan metode kuantitatif experiment dengan jenis penelitian quasi experiment. Teknik pengumpulan data pada penelitian ini yaitu tes, observasi dan dokumentasi. Sampel yang digunakan pada penelitian ini yaitu sebanyak 30 anak yang terdiri dari 15 anak sebagai kelompok eksperimen dan 15 anak sebagai kelompok kontrol. Teknik analisis data statistic yaitu dengan menggunakan uji t-test yang menunjukan tingkat nilai signifikansi (2 tailed) sebesar 0,000 < 0,05. Hal ini telah menunjukan bahwa penggunaan multimedia berbasis android memiliki pengaruh terhadap kemampuan mengenal bentuk geometri anak usia 5-6 tahun. Hasil analisis pun telah disimpulkan bahwa rata-rata kelompok eksperimen pada pretest sebesar 0,226 > 0,05 dan posttest sebesar 0,281 > 0,05 . Maka dapat disimpulkan bahwa penggunaan multimedia berbasis android memiliki pengaruh terhadap kemampuan mengenal bentuk geometri pada anak usia 5-6 tahun dan kemampuan mengenal bentuk geometri telah meningkat. Kata Kunci: multimedia berbasis android, geometri, 5-6 tahun.
S-9-PAHSA regulates glycolipid metabolism by enhancing autophagy and upregulating PI3K/AKT pathway
Authors: Yu Zhong-yu , Lin Jin-hong , Wang Jian-tao , Liu Ying-chao , Zhang Xiao-ming , Wen Xiao-hong , Zhang Qian , Xu Xiao-die , He Cheng-feng , Xue Wen-jiao , Wang Xin-ru , Ren Jiao-qi , Wang Jin-xiu , Wang Yin , Xiao Ji-chang , Guo Jing-chun , Zhou Hou-guang
Published: August 2023

The disorders of glucolipid metabolism are prevalent in patients with type 2 diabetes
mellitus (T2DM). Since the effects of Palmitic-Acid-Hydroxy-Stearic Acid (PAHSA)
on T2DM were not fully understood, the study aimed to investigate the role of S-9-
PAHSA in diabetes and the possible mechanisms. Male C57BL6 mice were fed with a
high-fat diet (HFD) and injected with streptozotocin (STZ) to establish the diabetic
mice model, and then administered with low- or high-dose S-9-PAHSA. Insulin,
glycosylated serum protein (GSP), glucagon-like peptide-1 (GLP-1), and oxidized lowdensity
lipoprotein (ox-LDL) were assessed by ELISA. SH-SY 5Y cells were incubated
in a diabetic environment and treated with S-9-PAHSA, R-9-PAHSA, or 9-PAHSA.
Cell viability, cell proliferation, apoptosis, lactate dehydrogenase (LDH), and reactive
oxygen species (ROS) were measured by corresponding kits. Protein expressions were
determined by western blot. The results showed high-does S-9-PAHSA improved
insulin resistance and lowered ox-LDL. Compared with the control group, the
significantly up-regulated expression of p62 and down-regulated expression of beclin
1 were found in diabetic mice and could be reversed by S-9-PAHSA. In vitro, S-9-
PAHSA increased cell viability and proliferation while reducing LDH and ROS. S-9-
PAHSA could reduce the expression of cleaved caspase-3 and TUNEL-positive cells,
and increase the ratio of LC3II/I after high glucose and fatty acid stimulation in vitro.
Downward trends were observed in PI3K and the phosphorylation of AKT in diabetic
environments, which could be slightly restored by S-9-PAHSA. In conclusion, S-9-
PAHSA could regulate glycolipid metabolism both in vivo and in vitro, indicating the
vital role of S-9-PAHSA in T2DM.
RCTrep: An R Package for the Validation of Estimates of the Average Treatment Effect
Authors: Shen Lingjie , Geleijnse Gijs , Kaptein Maurits
Published: August 2023

Despite the recent development of numerous methods aiming to estimate individual-level treatment effects based on observational data, assessing the validity of these estimates remains challenging. It is often unclear whether the observational data meet the assumptions imposed by a method. Additionally, there is often great flexibility in model choice when implementing a given method. This article introduces the R package RCTrep, designed for easy assessment of the validity of estimates of the average treatment effect obtained from observational data. This is achieved by a) making it easy to obtain and visualize estimates derived using a large variety of methods, and b) ensuring that these estimates are easily compared to a gold standard on population and subpopulation levels. RCTrep outlines a four-step workflow, namely, set-selection, estimation, diagnosis, and validation. The package provides a simple dashboard to review the obtained results. This article serves as a user guide for researchers aiming to leverage the potential of observational data to inform personalized treatment.
A novel study on the adsorption of low concentration Cd(II) and Ni(II) using porous boron nitride: effectiveness, coexisting anion interference, and regeneration
Authors: Han Fei , Zhao Mingyao , Ding Xun
Published: August 2023

The difficulty of removing low-concentration heavy metals from wastewater and the impact of coexisting anions on adsorption and regeneration performance has been widely recognized. To address this challenge, we synthesized and characterized a new adsorbent called porous boron nitride (PBN) using various techniques such as X-ray diffraction, Scanning Electron Microscopy, Fourier transform infrared spectroscopy and Brunner-Emmet-Teller measurements. We studied the adsorption kinetics and isotherms of Cd(II) and Ni(II) by PBN in the presence of interference anions using three kinetic models and four isothermal models. Our results showed that PBN reached adsorption equilibrium for Cd(II) and Ni(II) with a concentration of 10 mg/L within 5 minutes, and that the adsorption processes fit better with the pseudo-second order kinetic model and the Freundlich isothermal model. Furthermore, we found that the presence of SO42− inhibited the adsorption of Cd(II) and Ni(II), whereas SiO32−, CO32−, and PO43− promoted adsorption by forming a PBN-anion-metal ternary complex. We determined that the adsorption mechanism involved electrostatic attraction and chemisorption. After regeneration, PBN retained its crystal structure and typical pore distribution, demonstrating excellent adsorption performance for heavy metals.
Design of MIMO Microstrip Patch Antenna for 5G Applications
Authors: M Suresh Chinnathampy , A Ancy Marzla , L Dhivya Priya E , V Sneha
Published: August 2023

In this paper, a Microstrip Patch Antenna (MPA) is about to be designed. A dielectric material known as the substrate is sandwiched between a metallic patch and ground in an MPA. In comparison to the substrate and ground, a patch is smaller in size. The resonant frequency and dielectric constant value affect the size of a microstrip patch antenna. The proposed technology is a multi- input and multi-output microstrip patch antenna technology for wireless communications. The term "multiple inputs and multiple outputs" (MIMO) refers to a wireless technology that transfers more data between the transmitter and receiver at once to speed up data transmission and reduce mistakes. In essence, this idea refers to wireless network technology enabling access points or wireless routers to have many antennas. Simulating all antenna parameters, such as VSWR, balance, efficiencies, far field, and s-parameters, using CST Microwave Studio is the only way to verify the proposed antennas' performance.
HS-HA: Design of High-Speed Hardware Accelerator SOC for Biomedical Applications
Authors: Mavinkattimath Swati , Khanai Rajashri , Torse Dattaprasad , Iyer Nalini
Published: August 2023

Wireless Body Sensor Networks (WBSNs) consists of a Microcontroller used to process biosignals from sensors connected in or on the body. However, one of the issues observed in traditional WBSN is speed, which is addressed specifically in this work. This paper explains the operation of a Hardware Accelerator (HA) for WBSN consisting of four processing techniques Register bank, Predictor, Encryption, and Error Control Coding (ECC) implemented using Field Programmable Gate Array (FPGA) board PYNQ Z2 by TUL corporation. The improvements in the design of WBSN using HA are observed through the results of synthesis and implementation. The values of Worst Negative Slack (WNS) and utilization report represents the improvements in the design. HA’s floor planning and routing during Hardware-Software co-design produced a WNS of 0.269 ns. The proposed HA design in this paper utilizes 5.69k gate counts and consumes 0.227mW of power when operating at 250 MHz by using a 28-nm CMOS process.
Full-fat corn germ improves the performance and milk quality of lactating cows fed sugarcane bagasse and cactus cladodes as forage sources
Authors: Silva Camila , Gama Marco Antônio S. da , Silva Erick Alexandre M. , Ribeiro Emília F. , Felix Silas B. , Monteiro Carolina C.F. , Mora-Luna Robert E. , de Oliveira Júlio Cesar V. , Santos Djalma C. dos , Ferreira Marcelo de A.
Published: August 2023

the present study investigated the effects of replacing ground corn with full-fat corn germ on milk production, milk composition, and nutrient utilization in cows fed sugarcane bagasse and cactus cladodes. Ten multiparous Girolando cows (average body weight 500 ± 66 kg, 90 ± 15 days in milk) were distributed into a replicated 5 x 5 Latin Square, and assigned to five dietary treatments containing 0%, 25%, 50%, 75%, or 100% of full-fat corn germ in substitution to ground corn. Full-fat corn germ increased energy-corrected milk yield and synthesis of fat, lactose, and total solids in milk (p < 0.05). Cows fed corn germ quadratically increased (p < 0.05) intake of dry matter, crude protein, and total digestible nutrients, and linearly reduced (p < 0.05) the intake of total non-fiber carbohydrates. Except for ether extract and non-fiber carbohydrates, full-fat corn germ did not alter (p > 0.05) nutrient digestibility. Feeding corn germ reduced (p < 0.05) the excretion of urea-N in milk and N excretion via urine. These results demonstrate that full-fat corn germ can partially replace ground corn to enhance production efficiency in crossbred cows fed cactus cladodes and sugarcane bagasse. Furthermore, including sugarcane bagasse in FFCG-supplemented diets prevents milk fat depression in cows fed cactus cladodes.
Elimination of the RETNLB Gene Alleviates Intestinal Damage in NEC Mice
Authors: Xu Fen , Jin Zhenchao , Luo Jun
Published: August 2023

Background: The most serious gastrointestinal inflammation in newborns is necrotizing enterocolitis (NEC), which progresses quickly and has a high fatality rate. Resistin related molecule β (RELMβ) is highly concentrated in the intestines of both mice and humans according to earlier studies that have linked RELMβ to a variety of intestinal inflammatory disorders. Therefore, we hypothesized that targeting the RELMβ gene may inhibit inflammation and alleviate NEC in vivo.
Methods: First, RELMβ (RETNLB) knockout (KO) C57BL/6 mice were procured. Then, RETNLB KO mice and wild-type (WT) C57BL/6 mice were separated into 4 groups according to the NEC experimental model used for this study. The groups were as follows: group A consisted of WT mice, group B consisted of KO mice, group C consisted of WT mice with NEC, and Group D consisted of KO mice with NEC. Each group had 9 mice, and alterations in intestinal histology were analyzed. Expression levels of TLR2 and TLR4 in the intestinal tissues of each group were evaluated by simultaneous quantitative real-time polymerase chain reaction (qRT-PCR).
Results: Compared with WT mice, the expression levels of TLR2 and TLR4 were significantly up-regulated in WT mice following induction of NEC. Post-RETNLB KO, the expression levels of TLR2 and TLR4 in the KO group decreased significantly, and the differences were determined to be statistically significant (P<0.05). Interestingly, the degree of pathological damage to the intestines of KO mice was attenuated after induction of NEC. Compared with KO mice, there was no significant difference in the expression level of TLR4 in the KO NEC group (P>0.05); however, the expression level of TLR2 was significantly up-regulated (P<0.05).
Conclusion: RETNLB KO may exert a protective effect against intestinal injury in NEC mice through regulation of TLR4.
Extracurricular Physical Exercise Affects Teenagers' Expectation of Self-education and mechanism analysis-- Evidence from the CEPS Data
Authors: Xiao Qin , Tang Fengyun
Published: August 2023

Background: Extracurricular physical exercise is important in improving teenagers' expectations of self-education. However, in China, there are limited empirical analyses based on large microdata samples.
Methods: In this study, CEPS data and Ologit model are used to investigate the effect and mechanism of Extracurricular physical exercise on teenagers' self-education expectations.
Results: Extracurricular physical exercise can significantly improve teenagers' expectations of self-education; however, there are significant differences in the influence on teenagers with different characteristics. It can significantly increase the expectation of self-education among adolescents with different health levels, moderate and lower cognitive ability, moderate academic performance, relative confidence, and persistence while significantly decreasing the expectation of self-education among adolescents with poor academic performance. It does not significantly affect the self-education expectations of adolescents with higher cognitive ability and better academic performance.
Conclusions: The improvement of cognitive ability and academic performance, health level, and self-confidence are all important channels for the improvement of teenagers' expectations of self-education through extracurricular physical exercise.
Early versus Delayed Mobilization for Arthroscopic Rotator Cuff Repair: a Meta-analysis of Randomized Controlled Trials
Authors: Hu Ching-Wei , Tsai Sung Huang Laurent , Chen Chien-Hao , Tang Hao-Che , Su Chun-Yi , Tischler Eric H , Yang Yi-Chiang , Chan Yi-Sheng , Chiu Chih-Hao , Chen Alvin Chao-Yu
Published: August 2023

Background
The timing to start passive or active range of motion (ROM) after arthroscopic rotator cuff repair remains unclear. This systematic review and meta-analysis evaluates early versus delayed passive and active ROM protocols following arthroscopic rotator cuff repair. The aim of this study is to systematically review the literature on the outcomes of early active/passive versus delayed active/passive postoperative arthroscopic rotator cuff repair rehabilitation protocols.
Methods
A systematic review and meta-analysis of randomized controlled trials (RCTs) published up to April 2022 comparing early motion (EM) versus delayed motion (DM) rehabilitation protocols after arthroscopic rotator cuff repair for partial and full thickness tear was conducted. The primary outcome was range of motion (anterior flexion, external rotation, internal rotation, abduction) and the secondary outcomes were Constant-Murley score (CMS), Simple Shoulder Test Score (SST score) and Visual Analogue Scale (VAS).
Results
Thirteen RCTs with 1,082 patients were included in this study (7 RCTs for early passive motion (EPM) vs. delayed passive motion (DPM) and 7 RCTs for early active motion (EAM) vs. delayed active motion (DAM). Anterior flexion (1.40, 95% confidence interval (CI), 0.55–2.25) and abduction (2.73, 95%CI, 0.74–4.71) were higher in the EPM group compared to DPM. Similarly, EAM showed superiority in anterior flexion (1.57, 95%CI, 0.62–2.52) and external rotation (1.59, 95%CI, 0.36–2.82), compared to DAM. There was no difference between EPM and DPM for external rotation, retear rate, CMS and SST scores. There was no difference between EAM and DAM for retear rate, abduction, CMS and VAS.
Conclusion
EAM and EPM were both associated with superior ROM compared to the DAM and DPM protocols. Early mobilization may be suggested to improve ROM after arthroscopic surgery.
Endotaxial Stabilization of 2D Charge Density Waves with Long-range Order
Authors: Hovden Robert , Sung Suk Hyun , Agarwal Nishkarsh , Baggari Ismail El , Goh Yin Min , Kezer Patrick , Schnitzer Noah , Liu Yu , Lu Wenjian , Sun Yuping , Kourkoutis Lena , Heron John , Sun Kai
Published: August 2023

Charge density waves are emergent quantum states that spontaneously reduce crystal symmetry, drive metal-insulator transitions, and precede superconductivity. In low-dimensions, distinct quantum states arise, however, thermal fluctuations and external disorder destroy long-range order. Here we stabilize ordered two-dimensional (2D) charge density waves through endotaxial synthesis of confined monolayers of 1T-TaS2. Specifically, an ordered incommensurate charge density wave (oIC-CDW) is realized in 2D with dramatically enhanced amplitude and resistivity. By enhancing CDW order, the hexatic nature of charge density waves becomes observable. Upon heating via in-situ TEM, the CDW continuously melts in a reversible hexatic process wherein topological defects form in the charge density wave. From these results, new regimes of the CDW phase diagram for 1T-TaS2 are derived and consistent with the predicted emergence of vestigial quantum order.
Intake, digestibility, milk production, ingestive behavior and gas emissions of Saanen goats fed pornunça silage with levels of condensed tannin extracted from Schinopsis brasiliensis
Authors: Menezes Daniel Ribeiro , Carvalho Dalinne Tamara Queiroz , de Lucena Alita Ruth Ferraz , Marcelino Polyana Deyse Rodrigues , de Moura Lais Micaele Lopes , Queiroz Mário Adriano Ávila , de Moraes Salete Alves , Gois Glayciane Costa , Cruz Cláudia Horne da , Lima Anny Graycy Vasconcelos de Oliveira , Nascimento Thiago Vinicius Costa
Published: August 2023

The objective of this study was to determine the greatest concentration of condensed tannin (CT) extracted from Schinopsis brasiliensis in pornunça silages based on intake, digestibility, milk production, ingestive behavior and estimated greenhouse gas (GHG) emissions in dairy goats. Eight multiparous Saanen goats at 30 days of lactation, with 38.0 kg of initial body weight, production of 2.0 ± 0.4 kg of milk/day, mean age of 2–3 years, were distributed in a double Latin square (4x4) with four experimental periods and four diets with different tannin levels at concentrations 0% (control), 2.4, 3.6 and 4.8% tannin of total diet. The CT levels in the silage of pornunça promoted a linear decrease for the intake of dry matter (DM, P < 0.001), organic matter (OM, P < 0.001), ash (P < 0.001), crude protein (CP, P < 0.001), neutral detergent fiber (NDF, P < 0.001), acid detergent fiber (ADF, P < 0.001) and water (P < 0.001). The inclusion of CT also promoted a linear decrease in apparent digestibility coefficients of DM (P < 0.001), OM (P < 0.001) and CP (P < 0.001), milk production (P < 0.001), NDF (P < 0.001), and GHG emission estimation (P < 0.001). The intake and rumination rates of DM and NDF showed a linear decrease (P < 0.001) with level of tannin in the diets. In the experimental conditions, the inclusion of condensed tannin extracted from Schinopsis brasiliensis in pornunça silages, at levels up to 2.4% DM basis, promotes a decrease in intake of DM (DMI) and OM (OMI) and milk production as decreases GHG emissions in Saanen goats.
Citral in Lemon myrtle, Lemongrass, Litsea, and Melissa essential oils suppress the growth and invasion of breast cancer cells
Authors: Nagata Takuya , Satou Satoshi , Hayashi Shinichiro , Satyal Prabodh , Watanabe Manabu , Riggs Brannick , Saida Yoshihisa
Published: August 2023

Objective
Although cancer therapy suppresses recurrence and prolongs life, it may be accompanied by strong side effects; thus, there is a strong demand for the development effective treatments with fewer side effects. Cancer therapy using plant-derived essential oils is attracting attention as one promising method. This study investigated the antitumor effects of essential oil volatiles on breast cancer cells and identifies four essential oils that display antitumor activity.
Methods
Breast cancer cells were cultured in a 96-well plate, then one of twenty essential oils was added dropwise to the central well. The plate was incubated at 37°C for 48 h and the effect of the volatile components of each essential oil on the surrounding breast cancer cell growth ability was examined using an MTT assay. Gas chromatography was used to investigate the concentration of the transpiration components that may affect cancer cells.
Results
Of the 20 essential oils, Lemongrass, Lemon myrtle, Litsea, and Melissa displayed strong anti-tumor effects. These essential oils inhibited the growth of nearby breast cancer cells, even when diluted more than 500-fold. The transpiration component of lemon Myrtle showed the strongest antitumor effect, but was the least cytotoxic to mononuclear cells in normal peripheral blood (PBMC). Each of these essential oils contained a very large amount of citral. The IC50 against breast cancer cells when citral was volatilized from each essential oil was 1.67 µL/mL for geranial and 1.31 µL/mL for neral. Volatilized citral alone showed strong anti-proliferation and infiltration-inhibiting effects.
Conclusion
The transpiration components of Lemongrass, Lemon myrtle, Litsea, and Melissa are thought to inhibit breast cancer cell proliferation due to their high levels of citral.
A survey on the current status of Helicobacter pylori infection in households in Hainan Province
Authors: Liu Danni , Pan Jing , Chen Zhengyi , Li Sailian , Ma Jiamei , Xiao Yening , Wang Danhong , Mu Ganggang , Lin Ya , Li Juyuan , Chen Zhai , Huang Xiaoxi
Published: August 2023

Objective
This study aims to assess the prevalence of Helicobacter pylori (Hp) infection at the household level in Hainan Province and identify the factors that contribute to its spread. The findings of this study have significant implications for public health prevention strategies in the Hainan region.
Methods
A total of 421 families, comprising 1355 individuals, were tested for Hp infection across five cities in Hainan Province between July 2021 and April 2022. The study utilized questionnaires to identify potential factors linked to household Hp infection and transmission patterns.
Results
The prevalence of Hp infection on an individual basis was 46.72% (629/1355), with age ≥ 20 years, being married and having junior secondary education and above as risk factors for Hp infection. The prevalence of Hp infection in households was 80.29% (338/421), household size of 5, 6 and above were risk factors for Hp infection with Odds Ratios (ORs) of 4.09 (1.17–14.33) and 15.19 (2.01-114.73), respectively, household income ≥ 100,000 yuan and drinking boiled water from a tap source were protective factors for Hp infection with ORs of 0.52 (0.31–0.89) and 0.51 (0.28–0.95), respectively. The prevalence of Hp infection among minors in the household was 24.89% (58/233), with paternal infection and maternal infection as risk factors for child infection, with ORs of 2.93 (1.29–6.62) and 2.51 (1.07–5.89), respectively.
Conclusion
Hp infection was prevalent among Hainan families, and interaction with infected family members may be the primary cause of transmission.
Enhancing Healthcare Workers' Preparedness In Covid-19 Pandemic Response: Role of Pre-Pandemic Training Programmes on Personal Protective Equipment (PPE)
Authors: Haq Zia Ul , Sher Zarghona Fazal , Khattak Farhad Ali Khattak Ali , Khan Zala , Hakim Muhammad , Ullah Naeem , Rahim Abid , Hussain Umar , Afaq Saima
Published: August 2023

Background: The COVID-19 pandemic has revealed vulnerabilities in healthcare systems worldwide, emphasizing the importance of healthcare worker safety through adequate utilization of personal protective equipment (PPE). This study aims to assess the impact of pre-pandemic PPE training on the practices of frontline healthcare workers during the COVID-19 pandemic in Pakistan and provide insights into the effectiveness of such training programs for future initiatives.
Methods: A cross-sectional study was conducted among the frontline healthcare workers against COVID-19 in Pakistan, utilizing an online structured questionnaire shared via WhatsApp and Facebook. Statistical analyses, including chi-square tests for proportion and logistic regression for the association while multi-logistic regression for potential confounders, were performed using SPSS version 22.
Results: A total of 453 healthcare staff participated, with 68.9% (n=312) reporting no prior PPE training and 31.1% (n=141) having received training. Significant associations were found between prior training and healthcare group distribution (p=0.006), with doctors exhibiting the highest proportion of training 82 (37.61%), followed by nurses 50 (27.32%) and paramedics 9 (17.31%). The location also demonstrated a significant association (p=0.05), with Sind (48.00%) followed by Baluchistan (40.0%) reporting the highest proportions of prior training. Multi-logistic regression analysis identified paramedics as 0.26 times less likely to have received prior PPE training (Adjusted OR 0.26, 95% CI 0.10-0.65, p=0.01) compared to medical doctors. Healthcare workers in tertiary care hospitals were 0.46 times less likely to undergo PPE training (Adjusted OR 0.46, 95% CI 0.25-0.87,p=0.01) compared to those working at COVID-19 facilities/hospitals/quarantine centers.
Conclusion: Our findings highlight that healthcare levels, type of healthcare, and doffing skills are important predictors of whether healthcare workers have taken prior training in PPE. These findings imply developing effective training programs for healthcare workers to ensure safety while providing care during pandemics like COVID-19.
Use of double plate fixation with Masquelet technique fixation for large segmental bone defects of distal tibia: A Retrospective Study and Literature Review
Authors: Wang Zhaohui , Zhan Xiaohuan , Li Xiaohuan , Chen Guocai , Gao Junqing
Published: August 2023

Background
There is no effective consensus on the choice of internal fixation method for the Masquelet technique in the treatment of large segmental bone defects of the distal tibia. The aim of the study was to investigate the outcomes of the Masquelet technique combined with double plate fixation in the treatment of large segmental bone defects of the distal tibia.
Methods
This was a retrospective study. 21 patients with large segmental bone defects of the distal tibia were treated between June 2017 and June 2020. The length of bone defect ranged from 6.0 cm to 10.5 cm (mean, 7.8 cm). In the first stage of treatment, following complete debridement, a cement spacer was placed to induce membrane formation. In the second stage, double plate fixation and autologous cancellous bone graft were used for bone reconstruction. Each patient’s full weight-bearing time, bone healing time, and Iowa ankle score were recorded, and the occurrence of any complications was noted.
Results
All patients were followed up for 16 to 26 months (mean, 20.2 months). The group mean full weight-bearing time and bone healing time after bone grafting were 3.8 (± 0.7) months and 7.5 (± 0.8) months, respectively. During the treatment, one patient had a wound infection on the medial side of the leg, and the medial plate was removed. The wound healed after debridement, and the infection did not recur. After extraction of iliac bone for grafting, one patient had a large number of iliac bone defects, which were filled using cement spacer. Patients reported mild pain in the left bone extraction area after surgery. The Iowa ankle score was 84–94, versus preoperative and postoperative scores, P < 0.05, with 15 cases rated as “excellent” on this system, and 6 cases rated as “good.”
Conclusions
The Masquelet technique combined with double plate fixation is a safe and effective method for the repair of large segmental bone defects of the distal tibia.
Prognostic potential of preoperative circulating tumor cells to predict the early progression recurrence in hepatocellular carcinoma patients after hepatectomy
Authors: Lu Zhan , Ni Hanghang , Yang Xihua , Tan Lihao , Zhuang Haixiao , Mo Yunning , Wei Xingyu , Qi Lunnan , Xiang Bangde
Published: August 2023

Background The role of circulating tumor cells (CTCs) in prognosis prediction has been actively studied in hepatocellular carcinoma (HCC) patients. However, their efficiency in accurately predicting early progression recurrence (EPR) is unclear. This study aimed to investigate the clinical potential of preoperative CTCs to predict EPR in HCC patients after hepatectomy.
Methods 145 HCC patients, whose preoperative CTCs were detected, were enrolled. Based on the recurrence times and types, the patients were divided into four groups, including early oligo-recurrence (EOR), EPR, late oligo-recurrence (LOR), and late progression recurrence (LPR).
Results Among the 145 patients, 133 (91.7%) patients had a postoperative recurrence, including 51 EOR, 42 EPR, 39 LOR, and 1 LPR patient. Kaplan–Meier survival curve analysis indicated that the HCC patients with EPR had the worst OS. There were significant differences in the total-CTCs (T-CTCs) and CTCs subtypes count between the EPR group with EOR and LOR groups. Cox regression analysis indicated that the T-CTC count of >5/5 mL,the presence of microvascular invasion (MVI)and satellite nodules were the independent risk factors for EPR. The efficiency of T-CTCs was superior as compared to those of the other indicators in predicting EPR. Moreover, the combined model demonstrated a markedly superior area under the curve (AUC).
Conclusions the HCC patients with EPR had the worst OS. The preoperative CTCs might serve as a prognostic indicator of EPR for HCC patients. The combined models, including T-CTCs, MVI, and satellite nodules, had the best performance to predict EPR after hepatectomy.
Building climate resilience: Awareness of climate change adaptation in German Primary Care
Authors: Litke Nicola Alexandra , Poß-Doering Regina , Fehrer Valeska , Köppen Martina , Kümmel Stephanie , Szecsenyi Joachim , Wensing Michel
Published: August 2023

Background:Climate change is seen as the biggest health threat of the 21st century. Making primary care institutions resilient is therefore crucial to protect vulnerable groups and maintain quality of care. Awareness is a precondition for action. This study aims to explore awareness (knowledge, experience and attention) of climate change adaptation among primary care stakeholders.
Methods:Semi-structured interviews and focus groups with primary care stakeholders were conducted. The qualitative data were analysed in a two-step Thematic Analysis process.
Results: In total, n=40 stakeholders participated in two focus groups and 26 interviews. The findings show a mixed degree of awareness in primary care institutions. The spectrum ranged from a passive role with curative acting only, handing over responsibility to others and a low perceived self-efficacy to a proactive and responsible implementation of adaptation strategies. Participants who saw the need and responsibility of climate change adaptation in primary care perceived low additional workload. In general, implementation of climate change adaptation measures and general awareness of climate change adaptation appeared to be depending on a certain tension for change and a higher self-efficacy.
Conclusion: Primary care plays a crucial role in climate change adaptation, and awareness needs to be increased further in order to cope with consequences of climate change. To facilitate this, there should be a strong emphasis on climate change adaptation strategies being part of healthcare provider roles rather than being perceived as an “add-on” to already high workloads.
The Relational Approach to Treating Self-Harm (RELATE): Study protocol for a Feasibility Randomised Controlled Trial Study of Cognitive Analytic Therapy for Adults who Self-Harm versus Treatment at Usual
Authors: Taylor Peter , Adeyemi Isabel , Marlow Katie , Cottam Sarah , Airnes Zerena , Hartley Samantha , Howells Victoria , Dunn Barney , Elliott Rachel , Hann Mark , Latham Cameron , Robinson Catherine , Turpin Clive , Kellett Stephen
Published: August 2023

Background
Self-harm is a prevalent behaviour that has a major detrimental impact on a person’s life. Psychological therapies have the potential to help, but evidence of effective interventions remains limited. Access and acceptability of interventions can also be a significant challenge, with individuals either being unable to access help, or having to endure long waiting-lists. Cognitive analytic therapy (CAT) is a time-limited and relationally-focused psychotherapy that may provide a valuable treatment option for people who self-harm. This protocol outlines the methodology for the first feasibility randomised controlled trial (RCT) of CAT for adults that self-harm. The trial will aim to determine the feasibility, acceptability and safety of undertaking larger-scale evaluations of CAT for self-harm within an RCT context.
Method
An RCT design with 1:1 allocation to CAT plus treatment as usual (TAU) or TAU alone. Participants will be adult outpatients with three or more instances of self-harm in the past year (target sample of n = 60). CAT will be 8 one-to-one weekly 60-minute sessions plus a follow-up session up to 8 weeks after the last session. Assessments will occur at baseline, 12 weeks and 18 weeks after randomisation. Qualitive interviews with participants will gain insights into feasibility and acceptability of CAT. Feasibility outcomes will be judged against progression criteria.
Discussion
CAT may be an effective and accessible treatment option for people who self-harm, providing a more relationally orientated alternative to more behavioural therapies. The proposed feasibility RCT is an important first step in evaluating CAT as a treatment for self-harm.
Trial Registration:
The trial was pre-registered (21/10/22) on ISR CTN (ISRCTN code: ISRCTN75661422)
Prediction of postoperative complications after hepatectomy with dynamic monitoring of central venous oxygen saturation
Authors: Ida Shinya , Morita Yoshifumi , Matsumoto Akio , Muraki Ryuta , Kitajima Ryo , Furuhashi Satoru , Takeda Makoto , Kikuchi Hirotoshi , Hiramatsu Yoshihiro , Takeuchi Hiroya
Published: August 2023

Background
The usefulness of static monitoring using central venous pressure has been reported for anesthesia management in hepatectomy. It is unclear whether intra-hepatectomy dynamic monitoring can predict the postoperative course. We aimed to investigate the association between intraoperative dynamic monitoring and post-hepatectomy complications. Furthermore, we propose a novel anesthetic management strategy to reduce postoperative complication.
Methods
From 2018 to 2021, 93 patients underwent hepatectomy at our hospital. Fifty-three patients who underwent dynamic monitoring during hepatectomy were enrolled. Flo Trac system was used for dynamic monitoring. The baseline central venous oxygen saturation was defined as the average central venous oxygen saturation for 30 min after anesthesia induction. Central venous oxygen saturation fluctuation was defined as the difference between the baseline and minimum central venous oxygen saturation. Postoperative complications were evaluated using the comprehensive complication index (CCI).
Results
Patients with central venous oxygen saturation fluctuation ≥ 10% had significantly higher CCI scores (0 vs. 20.9: p = 0.043). Patients with higher CCI scores demonstrated significantly higher preoperative C-reactive protein-to-lymphocyte ratio (7.51 vs. 24.49: p = 0.039), intraoperative bleeding (105 vs. 581 ml: p = 0.008), number of patients with major hepatectomy (4/45 vs. 3/8: p = 0.028), and number of patients with central venous oxygen saturation fluctuation ≥ 10% (11/45 vs. 6/8; p = 0.010). Central venous oxygen saturation fluctuation ≥ 10% (odds ratio: 9.53, p = 0.016) was the only independent predictor of elevated CCI.
Conclusions
Central venous oxygen saturation fluctuation during hepatectomy is a predictor of postoperative complications. Anesthetic management based on intraoperative dynamic monitoring and suppression of central venous oxygen saturation fluctuation may be effective measures to prevent postoperative complications.
A randomized double-blind clinical trial on safety and efficacy of tauroursodeoxycholic acid (TUDCA) as add-on treatment in patients affected by amyotrophic lateral sclerosis (ALS): the statistical analysis plan of TUDCA-ALS trial
Authors: Lombardo Flavia L. , Alegiani Stefania Spila , Mayer Flavia , Cipriani Marta , Giudice Maria Lo , Ludolph Albert Christian , McDermott Christopher J. , Corcia Philippe , Damme Philip Van , Berg Leonard H. Van den , Hardiman Orla , Nicolini Gabriele , Vanacore Nicola , Dickie Brian , Albanese Alberto , Puopolo Maria
Published: August 2023

Background: Amyotrophic Lateral Sclerosis (ALS) is a highly debilitating neurodegenerative condition. Despite recent advancements in understanding the molecular mechanisms underlying ALS, there have been no significant improvements in therapeutic options for ALS patients in recent years. Currently, there is no cure for ALS, and the only approved treatment in Europe is riluzole, which has been shown to slow the disease progression and prolong survival by approximately three months. Recently, tauroursodeoxycholic acid (TUDCA) has emerged as a promising and effective treatment for neurodegenerative diseases due to its neuroprotective activities.
Methods: The ongoing TUDCA-ALS study is a double-blinded, parallel arms, placebo-controlled, randomized multicenter phase III trial with the aim to assess the efficacy and safety of TUDCA as add-on therapy to riluzole in patients with ALS. The primary outcome measure is defined as a minimum 20% improvement in the ALS Functional Rating Scale-Revised (ALSFRS-R) slope during the randomisation period (18 months) compared to the lead-in period (3 months), Randomization will be stratified by country. Primary analysis will be conducted based on the intention-to-treat principle. Data recruitment commenced on February 22, 2019, and was closed on 23rd December 2021. The database will be locked in September 2023.
Discussion: This paper provides a comprehensive description of the statistical analysis plan in order to ensure reproducibility of the analysis and avoid selective reporting of outcomes and data-driven analysis. Sensitivity analyses have been included in the protocol to assess the impact of intercurrent events related to the Coronavirus disease 2019. By focusing on clinically meaningful and robust outcomes, this trial aims to determine whether TUDCA can be effective in slowing the disease progression in patients with ALS.
Trial registration: ClinicalTrials.gov NCT03800524. Registered on January 11, 2019, https://clinicaltrials.gov/study/NCT03800524
Earthquake knowledge level and sustainable earthquake awareness of university students
Authors: bayraktar demet turan , ÇOL Bahar KEFELİ , BAŞARAN Ayşe GÜMÜŞLER , KÖSE Burcu GENÇ
Published: August 2023

Purpose
The aim of this study is to determine the relationship between the earthquake knowledge level of university students and their sustainable earthquake awareness levels.
Materials and Methods
A descriptive-cross-sectional study was conducted in April 2023 with 546 students enrolled in the School of Health Services. Data were collected using the Student Information Form, Earthquake Knowledge Level Scale, and Sustainable Earthquake Awareness Scale. Percentages, means, standard deviations, Mann-Whitney U test, Kruskal-Wallis analysis, Bonferroni correction, Tamhane's T2 test, and Spearman correlation analysis were used for data evaluation. Prior to answering the study questions, informed consent was obtained from the students after obtaining ethical committee approval.
Findings
Among the participants, 82.1% were female, with a mean age of 20.78±2.17. 55.7% of the students had experienced an earthquake, 19.2% had lost a loved one in an earthquake, and 5.5% had participated in earthquake response efforts. The mean score on the Earthquake Knowledge Level scale was found to be 69.13±13.79, and the mean score on the Sustainable Earthquake Awareness scale was 64.65±14.89. The total score on the earthquake knowledge level scale was significantly higher among those who had participated in earthquake response efforts (p= .030). The knowledge score regarding the distribution of earthquake-prone regions was significantly higher among males and those who had participated in earthquake response efforts (p= .034, p= .002). The sub-dimension of knowledge about the effects of earthquakes was significantly higher among those who had experienced an earthquake, lost a loved one in an earthquake, and participated in earthquake response efforts (p= .000, p= .000, p= .001). There was no significant difference in the total and sub-dimension scores of the sustainable earthquake awareness scale among the independent variables. A positively moderate and significant correlation was found between earthquake knowledge level and sustainable earthquake awareness (r=.499, p<.001).
Conclusion:It was observed that the earthquake knowledge level of students was at a moderate level, while the sustainable earthquake awareness was below the moderate level. It can be stated that the earthquake knowledge level has an influence on earthquake awareness.
Lateral Heterometal Junction Rectifier Fabricated by Sequential Transmetallation of Coordination Nanosheet
Authors: Nishihara Hiroshi , Tan Choon Meng , Fukui Naoya , Takada Kenji , Maeda Hiroaki , Selezneva Ekaterina , Bourgès Cédric , Tsukagoshi Kazuhito , Mori Takao , Masunaga Hiroyasu , SASAKI Sono , Sirringhaus Henning
Published: August 2023

Heterostructures of two-dimensional materials realise novel and enhanced physical phenomena, making them attractive research targets. Compared to inorganic materials, coordination nanosheets have virtually infinite combinations, leading to tunability of physical properties and are promising candidates for heterostructure fabrication. Although stacking of coordination materials into vertical heterostructures is widely reported, reports of lateral coordination material heterostructures are few. Here we show the successful fabrication of a sharp and seamless lateral heterojunction showing diode behaviour, by sequential and spatially limited immersion of a new metalladithiolene coordination nanosheet, Zn3BHT, into aqueous Cu(II) and Fe(II) solutions. Upon immersion, the Zn centres in insulating Zn3BHT are replaced by Cu or Fe ions, resulting in remarkable increase of conductivity. The transmetallation is spatially confined, occurring only within the immersed area. We anticipate that our results will be a starting point towards exploring transmetallation of various two-dimensional materials to produce lateral heterojunctions, by providing a new and facile synthetic route.