bluuebunny/crossref_metadata_2025 huggingface dataset:
New Approaches to Diabetic Nephropathy from Bed to Bench
Authors: Tsai Jun-Li , Chen Cheng-Hsu , Wu Ming-Ju , Tsai Shang-Feng
Published: April 2022

Diabetic nephropathy (DN) is the main cause of end-stage kidney disease (ESKD). DN-related ESKD has the worst prognosis for survival compared with other causes. Due to the complex mechanisms of DN and the heterogeneous presentations, unmet needs exist for the renal outcome of diabetes mellitus. Clinical evidence for treating DN is rather solid. For example, the first Kidney Disease: Improving Global Outcomes (KDIGO) guideline was published in October 2020: KDIGO Clinical Practice Guideline for Diabetes Management in Chronic Kidney Disease. In December of 2020, the International Society of Nephrology published 60 (+1) breakthrough discoveries in nephrology. Among these breakthroughs, four important ones after 1980 were recognized, including glomerular hyperfiltration theory, renal protection by renin-angiotensin system inhibition, hypoxia-inducible factor, and sodium-glucose cotransporter 2 inhibitors. Here, we present a review on the pivotal and new mechanisms of DN from the implications of clinical studies and medications.
Alternative Splicing of Neuropeptide Prohormone and Receptor Genes Associated with Pain Sensitivity Was Detected with Zero-Inflated Models
Authors: Southey Bruce R. , Rodriguez-Zas Sandra L.
Published: April 2022

Migraine is often accompanied by exacerbated sensitivity to stimuli and pain associated with alternative splicing of genes in signaling pathways. Complementary analyses of alternative splicing of neuropeptide prohormone and receptor genes involved in cell–cell communication in the trigeminal ganglia and nucleus accumbens regions of mice presenting nitroglycerin-elicited hypersensitivity and control mice were conducted. De novo sequence assembly detected 540 isoforms from 168 neuropeptide prohormone and receptor genes. A zero-inflated negative binomial model that accommodates for potential excess of zero isoform counts enabled the detection of 27, 202, and 12 differentially expressed isoforms associated with hypersensitivity, regions, and the interaction between hypersensitivity and regions, respectively. Skipped exons and alternative 3′ splice sites were the most frequent splicing events detected in the genes studied. Significant differential splicing associated with hypersensitivity was identified in CALCA and VGF neuropeptide prohormone genes and ADCYAP1R1, CRHR2, and IGF1R neuropeptide receptor genes. The prevalent region effect on differential isoform levels (202 isoforms) and alternative splicing (82 events) were consistent with the distinct splicing known to differentiate central nervous structures. Our findings highlight the changes in alternative splicing in neuropeptide prohormone and receptor genes associated with hypersensitivity to pain and the necessity to target isoform profiles for enhanced understanding and treatment of associated disorders such as migraine.
Time Domain (TD) Proton NMR Analysis of the Oxidative Safety and Quality of Lipid-Rich Foods
Authors: Osheter Tatiana , Linder Charles , Wiesman Zeev
Published: April 2022

Food safety monitoring is highly important due to the generation of unhealthy components within many food products during harvesting, processing, storage, transportation and cooking. Current technologies for food safety analysis often require sample extraction and the modification of the complex chemical and morphological structures of foods, and are either time consuming, have insufficient component resolution or require costly and complex instrumentation. In addition to the detection of unhealthy chemical toxins and microbes, food safety needs further developments in (a) monitoring the optimal nutritional compositions in many different food categories and (b) minimizing the potential chemical changes of food components into unhealthy products at different stages from food production until digestion. Here, we review an efficient methodology for overcoming the present analytical limitations of monitoring a food’s composition, with an emphasis on oxidized food components, such as polyunsaturated fatty acids, in complex structures, including food emulsions, using compact instruments for simple real-time analysis. An intelligent low-field proton NMR as a time domain (TD) NMR relaxation sensor technology for the monitoring of T2 (spin-spin) and T1 (spin-lattice) energy relaxation times is reviewed to support decision-making by producers, retailers and consumers in regard to food safety and nutritional value during production, shipping, storage and consumption.
Ni Nanoparticles Embedded Ti3C2Tx-MXene Nanoarchitectures for Electrochemical Sensing of Methylmalonic Acid
Authors: Kumar Jai , Soomro Razium Ali , Neiber Rana R. , Ahmed Nazeer , Medany Shymaa S. , Albaqami Munirah D. , Nafady Ayman
Published: April 2022

MXenes-Ti3C2Tx, based on their versatile surface characteristics, has rapidly advanced as an interactive substrate to develop electrochemical sensors for clinical applications. Herein, Ni embedded Ti3C2Tx (MX−Ni) composites were prepared using a self-assembly approach where Ti3C2Tx sheets served as an interactive conductive substrate as well as a protective layer to nickel nanoparticles (Ni NPs), preventing their surface oxidation and aggregation. The composite displayed a cluster-like morphology with an intimate interfacial arrangement between Ni, Ti3C2Tx and Ti3C2Tx-derived TiO2. The configuration of MX−Ni into an electrochemical sensor realized a robust cathodic reduction current against methylmalonic acid (MMA), a biomarker to vitamin B12 deficiency. The synergism of Ni NPs strong redox characteristics with conductive Ti3C2Tx enabled sensitive signal output in wide detection ranges of 0.001 to 0.003 µM and 0.0035 to 0.017 µM and a detection sensitivity down to 0.12 pM of MMA. Importantly, the sensor demonstrated high signal reproducibility and excellent operational capabilities for MMA in a complex biological matrix such as human urine samples.
High Blood Parasite Infection Rate and Low Fitness Suggest That Forest Water Bodies Comprise Ecological Traps for Pied Flycatchers
Authors: Krams Ronalds , Krama Tatjana , Elferts Didzis , Daukšte Janīna , Raibarte Patrīcija , Brūmelis Guntis , Dauškane Iluta , Strode Linda , Krams Indrikis A.
Published: April 2022

Blood parasites are considered to have strong negative effects on host fitness. Negative fitness consequences may be associated with proximity to areas where blood parasite vectors reproduce. This study tested for relationships between haemosporidian infection prevalence, parasitemia, and fitness parameters of breeding Pied Flycatchers (Ficedula hypoleuca) at different distances from forest water bodies. Prevalence and parasitemias (the intensity of infection) of haemosporidians and vector abundance generally decreased with increasing distance from forest lakes, streams, and bogs. Fledgling numbers were lower, and their condition was worse in the vicinity of water bodies, compared with those located one kilometer away from lakes and streams. At the beginning of the breeding season, adult body mass was not related to distance to the nearest water body, whereas at the end of the breeding season body mass was significantly lower closer to water bodies. Forest areas around water bodies may represent ecological traps for Pied Flycatchers. Installing nest boxes in the vicinity of forest water bodies creates unintended ecological traps that may have conservation implications.
Critical Competences for the Management of Post-Operative Course in Patients with Digestive Tract Cancer: The Contribution of MADIT Methodology for a Nine-Month Longitudinal Study
Authors: Pinto Eleonora , Fabbian Alessandro , Alfieri Rita , Da Roit Anna , Marano Salvatore , Mattara Genny , Pilati Pierluigi , Castoro Carlo , Cavarzan Marco , Dalla Riva Marta Silvia , Orrù Luisa , Turchi Gian Piero
Published: April 2022

There is a high postoperative morbidity rate after cancer surgery, that impairs patients’ self-management, job condition and economic strength. This paper describes the results of a peculiar psychological intervention on patients undergoing surgery for esophageal, gastric and colorectal cancer. The intervention aimed to enhance patients’ competences in the management of postoperative daily life. A narrative approach (M.A.D.I.T.—Methodology for the Analysis of Computerised Text Data) was used to create a questionnaire, Health and Employment after Gastro-Intestinal Surgery—Dialogical Questionnaire, HEAGIS-DQ, that assesses four competences. It was administered to 48 participants. Results were used as guidance for specific intervention, structured on patients’ competence profiles. The intervention lasted nine months after surgery and was structured in weekly to monthly therapeutic sessions. Quality of Life questionnaires were administered too. At the end of the intervention, 94% of patients maintained their job and only 10% of patients asked for financial support. The mean self-perception of health-related quality of life was 71.2. The distribution of three of four competences increased after nine months (p < 0.05). Despite economic difficulties due to lasting symptoms after surgery, and to the current pandemic scenario, a structured intervention with patients let them to resume their jobs and continue activities after surgery.
The Impact of Life Trauma on Mental Health and Suicidal Behavior: A Study from Portuguese Language Countries
Authors: Silva Mariana , Pereira Henrique
Published: April 2022

Several studies report the incidence of traumatic experiences in community and clinical samples, and substantial research demonstrates the impact of traumatic events on mental health and suicidal behavior, but this area remains unexplored in the Community of Portuguese Language Countries (CPLC). Thus, this study aims to (1) describe traumatic experiences, mental health levels and suicidal behavior among individuals from Portugal, Brazil and African Countries with Portuguese as an Official Language (ACPOL); (2) assess correlations between traumatic experiences and mental health and suicidal behavior; and (3) assess the impact of exposure to a traumatic event on mental health and suicidal behavior. The measurement instruments included a sociodemographic questionnaire, Brief Trauma Questionnaire, Brief Symptoms Inventory-18, and the Portuguese version of the Suicidal Behaviors Questionnaire-Revised. ACPOL participants reported greater impact of war, Portuguese participants reported greater impact of disasters, and Brazilian participants reported greater impact of psychological and sexual abuse, assault, and death of a family member. Brazilian participants showed the worst levels of mental health and suicidal thoughts. Strong correlations were found between traumatic experiences and mental health levels and suicidal behavior. Traumatic experiences contributed to the explanation of mental health levels and probability of committing suicide.
The New Times of Social Media Marketing in the B2B Framework
Authors: Lopes João , Oliveira José
Published: April 2022

Nowadays, we live in the age of Marketing 4.0. Historically, marketing has often depended on changing consumer habits and needs. Thus, it is necessary to understand the new habits and needs of the consumer to make companies more and more effective. Currently, social media marketing (SMM) is ubiquitous in organizations, and is seen as a tool to achieve strategic goals. Therefore, SMM is important for companies to adapt their approach to customer relationship management and advance new marketing competencies to enable customer satisfaction. To drive customer satisfaction and improve customer experience, managers are loading social media applications into their current customer relationship management (CRM) systems. This study seeks to understand the feasibility of implementing SMM in business to business (B2B) companies and how this affects CRM and customer knowledge management (CKM). For this study, data were collected from two Portuguese micro-companies. The methodology used was exploratory qualitative in nature through a multiple case study, wherein semi-structured interviews were applied. It was found that SMM cannot be used in the B2B companies under investigation because CRM needs are much more dependent on CKM than on the mass interactions and indirect communication with the customer that can potentially exist in SMM. In these companies, communication with the client can be adjusted on a case-by-case basis through SMM, without the need for mass communication with all clients. This paper provides implications for the management of micro-enterprises regarding social media marketing. It also contributes to the development of the literature on SMM, CRM and CKM.
Aspirin-Triggered Resolvin D1 Reduces Chronic Dust-Induced Lung Pathology without Altering Susceptibility to Dust-Enhanced Carcinogenesis
Authors: Dominguez Edward C. , Phandthong Rattapol , Nguyen Matthew , Ulu Arzu , Guardado Stephanie , Sveiven Stefanie , Talbot Prue , Nordgren Tara M.
Published: April 2022

Lung cancer is the leading cause of cancer-related deaths worldwide, with increased risk being associated with unresolved or chronic inflammation. Agricultural and livestock workers endure significant exposure to agricultural dusts on a routine basis; however, the chronic inflammatory and carcinogenic effects of these dust exposure is unclear. We have developed a chronic dust exposure model of lung carcinogenesis in which mice were intranasally challenged three times a week for 24 weeks, using an aqueous dust extract (HDE) made from dust collected in swine confinement facilities. We also treated mice with the omega-3-fatty acid lipid mediator, aspirin-triggered resolvin D1 (AT-RvD1) to provide a novel therapeutic strategy for mitigating the inflammatory and carcinogenic effects of HDE. Exposure to HDE resulted in significant immune cell influx into the lungs, enhanced lung tumorigenesis, severe tissue pathogenesis, and a pro-inflammatory and carcinogenic gene signature, relative to saline-exposed mice. AT-RvD1 treatment mitigated the dust-induced inflammatory response but did not protect against HDE + NNK-enhanced tumorigenesis. Our data suggest that chronic HDE exposure induces a significant inflammatory and pro-carcinogenic response, whereas treatment with AT-RvD1 dampens the inflammatory responses, providing a strong argument for the therapeutic use of AT-RvD1 to mitigate chronic inflammation.
Proteoglycans in Cancer: Friends or Enemies? A Special Focus on Hepatocellular Carcinoma
Authors: Dituri Francesco , Gigante Gianluigi , Scialpi Rosanna , Mancarella Serena , Fabregat Isabel , Giannelli Gianluigi
Published: April 2022

Proteoglycans are a class of highly glycosylated proteins expressed in virtually all tissues, which are localized within membranes, but more often in the pericellular space and extracellular matrix (ECM), and are involved in tissue homeostasis and remodeling of the stromal microenvironment during physiological and pathological processes, such as tissue regeneration, angiogenesis, and cancer. In general, proteoglycans can perform signaling activities and influence a range of physical, chemical, and biological tissue properties, including the diffusivity of small electrolytes and nutrients and the bioavailability of growth factors. While the dysregulated expression of some proteoglycans is observed in many cancers, whether they act as supporters or limiters of neoplastic progression is still a matter of controversy, as the tumor promoting or suppressive function of some proteoglycans is context dependent. The participation of multiple proteoglycans in organ regeneration (as demonstrated for the liver in hepatectomy mouse models) and in cancer suggests that these molecules actively influence cell growth and motility, thus contributing to key events that characterize neoplastic progression. In this review, we outline the main roles of proteoglycans in the physiology and pathology of cancers, with a special mention to hepatocellular carcinoma (HCC), highlighting the translational potential of proteoglycans as targets or therapeutic agents for the treatment of this disease.
Epigenetic Determinants of Racial Disparity in Breast Cancer: Looking beyond Genetic Alterations
Authors: Joshi Shriya , Garlapati Chakravarthy , Aneja Ritu
Published: April 2022

Breast cancer (BC) is the most commonly diagnosed cancer in women. Despite advancements in BC screening, prevention, and treatment, BC incidence and mortality remain high among African American (AA) women. Compared with European American (EA) women, AA women tend to be diagnosed with more advanced and aggressive tumors and exhibit worse survival outcomes. Most studies investigating the determinants of racial disparities in BC have focused on genetic factors associated with African ancestry. However, various environmental and social stressors over an individual’s life course can also shape racial stratification in BC. These social and environmental exposures result in long-term changes in gene expression mediated by epigenetic mechanisms. Epigenetics is often portrayed as an intersection of socially patterned stress and genetic expression. The enduring nature of epigenetic changes makes them suitable for studying the effects of different environmental exposures over an individual’s life course on gene expression. The role of differential social and environmental exposures in racial disparities in BC suggests varied epigenetic profiles or signatures associated with specific BC subtypes in AA and EA women. These epigenetic profiles in EA and AA women could be used as biomarkers for early BC diagnosis and disease prognosis and may prove valuable for the development of targeted therapies for BC. This review article discusses the current state of knowledge regarding epigenetic differences between AA and EA women with BC. We also discuss the role of socio-environmental factors, including psychosocial stress, environmental toxicants, and dietary factors, in delineating the different epigenetic profiles in AA and EA patients with BC.
Deciphering Genetic Alterations of Hairy Cell Leukemia and Hairy Cell Leukemia-like Disorders in 98 Patients
Authors: Maitre Elsa , Tomowiak Cécile , Lebecque Benjamin , Bijou Fontanet , Benabed Khaled , Naguib Dina , Kerneves Pauline , Cornet Edouard , Viailly Pierre-Julien , Arsham Jeffrey , Sola Brigitte , Jardin Fabrice , Troussard Xavier
Published: April 2022

Hairy cell leukemia (cHCL) patients have, in most cases, a specific clinical and biological presentation with splenomegaly, anemia, leukopenia, neutropenia, monocytopenia and/or thrombocytopenia, identification of hairy cells that express CD103, CD123, CD25, CD11c and identification of the V600E mutation in the B-Raf proto-oncogene (BRAF) in 90% of cases. Monocytopenia is absent in vHCL and SDRPL patients and the abnormal cells do not express CD25 or CD123 and do not present the BRAFV600E mutation. Ten percent of cHCL patients are BRAFWT and the distinction between cHCL and HCL-like disorders including the variant form of HCL (vHCL) and splenic diffuse red pulp lymphoma (SDRPL) can be challenging. We performed deep sequencing in a large cohort of 84 cHCL and 16 HCL-like disorders to improve insights into the pathogenesis of the diseases. BRAF mutations were detected in 76/82 patients of cHCL (93%) and additional mutations were identified in Krüppel-like Factor 2 (KLF2) in 19 patients (23%) or CDKN1B in 6 patients (7.5%). Some KLF2 genetic alterations were localized on the cytidine deaminase (AID) consensus motif, suggesting AID-induced mutations. When analyzing sequential samples, a clonal evolution was identified in half of the cHCL patients (6/12 pts). Among the 16 patients with HCL-like disorders, we observed an enrichment of MAP2K1 mutations in vHCL/SDRPL (3/5 pts) and genes involved in the epigenetic regulation (KDM6A, EZH2, CREBBP, ARID1A) (3/5 pts). Furthermore, MAP2K1 mutations were associated with a bad prognosis and a shorter time to next treatment (TTNT) and progression-free survival (PFS), independently of the HCL classification.
Cannabinol Inhibits Cellular Proliferation, Invasion, and Angiogenesis of Neuroblastoma via Novel miR-34a/tRiMetF31/PFKFB3 Axis
Authors: Wang Bo , Li Dongping , Cherkasova Viktoriia , Gerasymchuk Marta , Narendran Aru , Kovalchuk Igor , Kovalchuk Olga
Published: April 2022

High-risk neuroblastoma is an aggressive pediatric tumor. Despite great advances in neuroblastoma therapy and supportive care protocols, no curative treatment is available for most patients with this disease. Here, we uncover that CBN attenuated the cell proliferation, invasion, and angiogenesis of neuroblastoma cell lines in a dose-dependent manner via the inhibition of the AKT pathway and the upregulation of miR-34a that targets E2F1. Both miR-34a and a 31-nt tRNAiMet fragment (tRiMetF31) derived from miR-34a-guided cleavage were downregulated in 4 examined neuroblastoma cell lines inversely correlated with the levels of its direct target, the PFKFB3 protein. Moreover, ectopic tRiMetF31 suppressed proliferation, migration, and angiogenesis in the studied neuroblastoma cell lines. Conversely, tRiMetF31 knockdown promoted PFKFB3 expression, resulting in enhanced angiogenesis. Our findings reveal a suppressive role of CBN in neuroblastoma tumorigenesis, highlighting a novel and crucial miR-34a tumor suppressor network in CBN’s antineuroblastoma actions.
MR-Guided Adaptive Radiotherapy for OAR Sparing in Head and Neck Cancers
Authors: Mulder Samuel L. , Heukelom Jolien , McDonald Brigid A. , Van Dijk Lisanne , Wahid Kareem A. , Sanders Keith , Salzillo Travis C. , Hemmati Mehdi , Schaefer Andrew , Fuller Clifton D.
Published: April 2022

MR-linac devices offer the potential for advancements in radiotherapy (RT) treatment of head and neck cancer (HNC) by using daily MR imaging performed at the time and setup of treatment delivery. This article aims to present a review of current adaptive RT (ART) methods on MR-Linac devices directed towards the sparing of organs at risk (OAR) and a view of future adaptive techniques seeking to improve the therapeutic ratio. This ratio expresses the relationship between the probability of tumor control and the probability of normal tissue damage and is thus an important conceptual metric of success in the sparing of OARs. Increasing spatial conformity of dose distributions to target volume and OARs is an initial step in achieving therapeutic improvements, followed by the use of imaging and clinical biomarkers to inform the clinical decision-making process in an ART paradigm. Pre-clinical and clinical findings support the incorporation of biomarkers into ART protocols and investment into further research to explore imaging biomarkers by taking advantage of the daily MR imaging workflow. A coherent understanding of this road map for RT in HNC is critical for directing future research efforts related to sparing OARs using image-guided radiotherapy (IGRT).
TR3 Enhances AR Variant Production and Transactivation, Promoting Androgen Independence of Prostate Cancer Cells
Authors: Tran Tuyen Thanh , Lee Keesook
Published: April 2022

The pro-oncogenic function of TR3, an orphan nuclear receptor, has been reported in prostate cancer. However, the roles of TR3 in androgen receptor (AR) expression and signaling in prostate cancer cells are poorly understood. Database analysis revealed that TR3 expression level is elevated in prostate tumors, and is positively, although weakly, correlated with that of AR. TR3 overexpression increased the production of AR splice variants in addition to general upregulation of AR expression. TR3 interacted with some spliceosomal complex components and AR precursor mRNA, altering the splice junction rates between exons. TR3 also enhanced androgen-independent AR function. Furthermore, TR3 overexpression increased cell proliferation and mobility of AR-positive prostate cancer cells and stimulated tumorigenesis of androgen-independent prostate cancer cells in mouse xenograft models. This is the first study to report that TR3 is a multifunctional regulator of AR signaling in prostate cancer cells. TR3 alters AR expression, splicing process, and activity in prostate cancer cells, increasing the androgen independence of AR signaling. Therefore, TR3 may play a crucial role in the progression of prostate cancer to an advanced castration-resistant form.
The Role of Radiotherapy in Treating Kaposi’s Sarcoma in HIV Infected Patients
Authors: Quéro Laurent , Palich Romain , Valantin Marc-Antoine ,   on behalf of CANCERVIH Working Group
Published: April 2022

Kaposi’s sarcoma (KS) is a radiosensitive cancer regardless of its form (classical, endemic, AIDS-related, and immunosuppressant therapy-related). Radiotherapy (RT) is an integral part of the therapeutic management of KS. RT may be used as the main treatment, in the case of solitary lesions, or as palliative therapy in the disseminated forms. The dose of RT to be delivered is 20–30 Gy by low-energy photons or by electrons. The complete response rate after RT is high, around 80–90%. This treatment is well tolerated. However, patients should be informed of the possible risk of the development of late skin sequelae and the possibility of recurrence. With the advent of highly active antiretroviral therapy (HAART), the indications for RT treatment in HIV-positive patients have decreased.
Outcome after Radiotherapy for Vestibular Schwannomas (VS)—Differences in Tumor Control, Symptoms and Quality of Life after Radiotherapy with Photon versus Proton Therapy
Authors: Küchler Maike , El Shafie Rami A. , Adeberg Sebastian , Herfarth Klaus , König Laila , Lang Kristin , Hörner-Rieber Juliane , Plinkert Peter Karl , Wick Wolfgang , Sahm Felix , Sprengel Simon David , Debus Jürgen , Bernhardt Denise
Published: April 2022

Background: To evaluate differences in local tumor control (LC), symptoms and quality of life (QOL) of 261 patients with VS after stereotactic radiosurgery/hypofractionated stereotactic radiotherapy (SRS/HFSRT) vs. fractionated radiotherapy (FRT) vs. fractionated proton therapy (FPT) were studied. Methods: For SRS/HFSRT (n = 149), the median fraction dose applied was 12 Gy. For FRT (n = 87) and FPT (n = 25), the median cumulative doses applied were 57.6 Gy and 54 Gy (RBE), respectively. FRT and FPT used single median doses of 1.8 Gy/Gy (RBE). Median follow-up was 38 months. We investigated dosimetry for organs at risk and analyzed toxicity and QOL by sending out a questionnaire. Results: LC was 99.5% at 12 months after RT with no statistical difference between treatment groups (p = 0.19). LC was significantly lower in NF2 patients (p = 0.004) and in patients with higher tumor extension grade (p = 0.039). The hearing preservation rate was 97% at 12 months after RT with no statistical difference between treatment groups (p = 0.31). Facial and trigeminal nerve affection after RT occurred as mild symptoms with highest toxicity rate in FPT patients. Conclusion: SRS/HFSRT, FRT and FPT for VS show similar overall clinical and functional outcomes. Cranial nerve impairment rates vary, potentially due to selection bias with larger VS in the FRT and FPT group.
Exploring the Potential of Water-Soluble Cu(II) Complexes with MPA–CdTe Quantum Dots for Photoinduced Electron Transfer
Authors: Botcha Niharika Krishna , Gutha Rithvik R. , Sadeghi Seyed M. , Mukherjee Anusree
Published: April 2022

Three water-soluble copper complexes based on the amine/pyridine functionalities were investigated, along with quantum dots, as a catalyst–photosensitizer assembly, respectively, for fundamental understanding of photoinduced electron transfer. Luminescence quenching and lifetime measurements were performed to try and establish the actual process that leads to the quenching, such as electron transfer, energy transfer, or complex formation (static quenching). Cyclic voltammetry and dynamic light scattering experiments were also performed. Irrespective of the similar reduction potentials of the three complexes, very different photoluminescence properties were observed.
Effect of UV Irradiation on the Structural Variation of Metal Oxide-Silica Nanocomposites for Enhanced Removal of Erythromycin at Neutral pH
Authors: Ghaffari Yasaman , Beak Soyoung , Bae Jiyeol , Saifuddin Md , Kim Kwang Soo
Published: April 2022

In this study, the effect of UV treatment on the physicochemical properties and structural variation of metal oxide-silica nanocomposites (Mn2O3-Fe2O3@SiO2) has been investigated. Based on the results, UV irradiation significantly affects the nanocomposite structure, where SiO2 network reconfiguration, change in surface OH group density, and surface area were observed. Erythromycin (ERY) has been chosen as a module pollutant to compare the performance of the pristine and UV-treated nanocomposites. The pristine nanocomposite had a high adsorption efficiency (99.47%) and photocatalytic activity (99.57%) at neutral pH for ERY in the first cycle, and this efficiency decreased significantly for the multiple cycles. However, different results have been observed for the UV-treated nanocomposite, where it retained its performance for ten consecutive cycles. This enhanced performance is attributed to the structural modifications after UV exposure, where increased surface area, pore volume, and OH group density resulted in an increased number of the possible mechanisms responsible for the adsorption/oxidation of ERY. Moreover, oxidation of adsorbed molecules by UV light after each cycle can also be another reason for enhanced removal. For the first time, the fate of ERY is studied using regenerated nanocomposites after the last cycle. LC/MS/MS results showed that ERY degraded in 20 min, and the produced reaction by-products were adsorbed by nanocomposites. This study could be a foundation research for the practical approaches for the regeneration of nanomaterials and the successful removal of organic pollutants from aquatic environments.
Expression of the Human Serotonin 5-HT7 Receptor Rescues Phenotype Profile and Restores Dysregulated Biomarkers in a Drosophila melanogaster Glioma Model
Authors: Courant Florestan , Maravat Marion , Chen Wanyin , Gosset David , Blot Lauren , Hervouet-Coste Nadège , Sarou-Kanian Vincent , Morisset-Lopez Séverine , Decoville Martine
Published: April 2022

Gliomas are the most common primary brain tumors in adults. Significant progress has been made in recent years in identifying the molecular alterations involved in gliomas. Among them, an amplification/overexpression of the EGFR (Epidermal Growth Factor Receptor) proto-oncogene and its associated signaling pathways have been widely described. However, current treatments remain ineffective for glioblastomas, the most severe forms. Thus, the identification of other pharmacological targets could open new therapeutic avenues. We used a glioma model in Drosophila melanogaster that results from the overexpression of constitutively active forms of EGFR and PI3K specifically in glial cells. We observed hyperproliferation of glial cells that leads to an increase in brain size and lethality at the third instar larval stage. After expression of the human serotonin 5-HT7 receptor in this glioma model, we observed a decrease in larval lethality associated with the presence of surviving adults and a return to a normal morphology of brain for some Drosophila. Those phenotypic changes are accompanied by the normalization of certain metabolic biomarkers measured by High-Resolution Magic Angle Spinning NMR (HR-MAS NMR). The 5-HT7R expression in glioma also restores some epigenetic modifications and characteristic markers of the signaling pathways associated with tumor growth. This study demonstrates the role of the serotonin 5-HT7 receptor as a tumor suppressor gene which is in agreement with transcriptomic analysis obtained on human glioblastomas.
Post-COVID Complications after Pressure Ulcer Surgery in Patients with Spinal Cord Injury Associate with Creatine Kinase Upregulation in Adipose Tissue
Authors: Martínez-Torija Mario , Esteban Pedro F. , Espino-Rodríguez Francisco Javier , Paniagua-Torija Beatriz , Molina-Holgado Eduardo , Ceruelo Silvia , Barroso-Garcia Gemma , Arandilla Alba G. , Lopez-Almodovar Luis F. , Arevalo-Martin Angel , Moreno Juan Antonio , Garcia-Ovejero Daniel , Durán-Ruiz Mª Carmen , Moreno-Luna Rafael
Published: April 2022

The risk of complications following surgical procedures is significantly increased in patients with SARS-CoV-2 infection. However, the mechanisms underlying these correlations are not fully known. Spinal cord injury (SCI) patients who underwent reconstructive surgery for pressure ulcers (PUs) before and during the COVID-19 pandemic were included in this study. The patient’s postoperative progression was registered, and the subcutaneous white adipose tissue (s-WAT) surrounding the ulcers was analyzed by proteomic and immunohistochemical assays to identify the molecular/cellular signatures of impaired recovery. Patients with SCI and a COVID-19-positive diagnosis showed worse recovery and severe postoperative complications, requiring reintervention. Several proteins were upregulated in the adipose tissue of these patients. Among them, CKMT2 and CKM stood out, and CKM increased for up to 60 days after the COVID-19 diagnosis. Moreover, CKMT2 and CKM were largely found in MGCs within the s-WAT of COVID patients. Some of these proteins presented post-translational modifications and were targeted by autoantibodies in the serum of COVID patients. Overall, our results indicate that CKMT2, CKM, and the presence of MGCs in the adipose tissue surrounding PUs in post-COVID patients could be predictive biomarkers of postsurgical complications. These results suggest that the inflammatory response in adipose tissue may underlie the defective repair seen after surgery.
Neuroprotective Effects of PARP Inhibitors in Drosophila Models of Alzheimer’s Disease
Authors: Maggiore Anna , Casale Assunta Maria , Toscanelli Walter , Cappucci Ugo , Rotili Dante , Grieco Maddalena , Gagné Jean-Philippe , Poirier Guy G. , d’Erme Maria , Piacentini Lucia
Published: April 2022

Alzheimer’s disease (AD) is an irreversible age-related neurodegenerative disorder clinically characterized by severe memory impairment, language deficits and cognitive decline. The major neuropathological hallmarks of AD include extracellular deposits of the β-amyloid (Aβ) peptides and cytoplasmic neurofibrillary tangles (NFTs) of hyperphosphorylated tau protein. The accumulation of plaques and tangles in the brain triggers a cascade of molecular events that culminate in neuronal damage and cell death. Despite extensive research, our understanding of the molecular basis of AD pathogenesis remains incomplete and a cure for this devastating disease is still not available. A growing body of evidence in different experimental models suggests that poly(ADP-ribose) polymerase-1 (PARP-1) overactivation might be a crucial component of the molecular network of interactions responsible for AD pathogenesis. In this work, we combined genetic, molecular and biochemical approaches to investigate the effects of two different PARP-1 inhibitors (olaparib and MC2050) in Drosophila models of Alzheimer’s disease by exploring their neuroprotective and therapeutic potential in vivo. We found that both pharmacological inhibition and genetic inactivation of PARP-1 significantly extend lifespan and improve the climbing ability of transgenic AD flies. Consistently, PARP-1 inhibitors lead to a significant decrease of Aβ42 aggregates and partially rescue the epigenetic alterations associated with AD in the brain. Interestingly, olaparib and MC2050 also suppress the AD-associated aberrant activation of transposable elements in neuronal tissues of AD flies.
Functional Study of TMEM163 Gene Variants Associated with Hypomyelination Leukodystrophy
Authors: Yan Huifang , Yang Shuyan , Hou Yiming , Ali Saima , Escobar Adrian , Gao Kai , Duan Ruoyu , Kubisiak Thomas , Wang Junyu , Zhang Yu , Xiao Jiangxi , Jiang Yuwu , Zhang Ting , Wu Ye , Burmeister Margit , Wang Qiang , Cuajungco Math P. , Wang Jingmin
Published: April 2022

Hypomyelinating leukodystrophies (HLDs) are a rare group of heterogeneously genetic disorders characterized by persistent deficit of myelin observed on magnetic resonance imaging (MRI). To identify a new disease-associated gene of HLD, trio-based whole exome sequencing was performed for unexplained patients with HLD. Functional studies were performed to confirm the phenotypic effect of candidate protein variants. Two de novo heterozygous variants, c.227T>G p.(L76R) or c.227T>C p.(L76P) in TMEM163 were identified in two unrelated HLD patients. TMEM163 protein is a zinc efflux transporter localized within the plasma membrane, lysosomes, early endosomes, and other vesicular compartments. It has not been associated with hypomyelination. Functional zinc flux assays in HeLa cells stably-expressing TMEM163 protein variants, L76R and L76P, revealed distinct attenuation or enhancement of zinc efflux, respectively. Experiments using a zebrafish model with knockdown of tmem163a and tmem163b (morphants) showed that loss of tmem163 causes dysplasia of the larvae, locomotor disability and myelin deficit. Expression of human wild type TMEM163 mRNAs in morphants rescues the phenotype, while the TMEM163 L76P and L76R mutants aggravated the condition. Moreover, poor proliferation, elevated apoptosis of oligodendrocytes, and reduced oligodendrocytes and neurons were also observed in zebrafish morphants. Our findings suggest an unappreciated role for TMEM163 protein in myelin development and add TMEM163 to a growing list of genes associated with hypomyelination leukodystrophy.
Effects of Oxygen Tension for Membrane Lipidome Remodeling of Cockayne Syndrome Cell Models
Authors: Ferreri Carla , Sansone Anna , Krokidis Marios G. , Masi Annalisa , Pascucci Barbara , D’Errico Mariarosaria , Chatgilialoglu Chryssostomos
Published: April 2022

Oxygen is important for lipid metabolism, being involved in both enzymatic transformations and oxidative reactivity, and is particularly influent when genetic diseases impair the repair machinery of the cells, such as described for Cockayne syndrome (CS). We used two cellular models of transformed fibroblasts defective for CSA and CSB genes and their normal counterparts, grown for 24 h under various oxygen tensions (hyperoxic 21%, physioxic 5% and hypoxic 1%) to examine the fatty acid-based membrane remodeling by GC analysis of fatty acid methyl esters derived from membrane phospholipids. Overall, we first distinguished differences due to oxygen tensions: (a) hyperoxia induced a general boost of desaturase enzymatic activity in both normal and defective CSA and CSB cell lines, increasing monounsaturated fatty acids (MUFA), whereas polyunsaturated fatty acids (PUFA) did not undergo oxidative consumption; (b) hypoxia slowed down desaturase activities, mostly in CSA cell lines and defective CSB, causing saturated fatty acids (SFA) to increase, whereas PUFA levels diminished, suggesting their involvement in hypoxia-related signaling. CSB-deprived cells are the most sensitive to oxidation and CSA-deprived cells are the most sensitive to the radical-based formation of trans fatty acids (TFA). The results point to the need to finely differentiate biological targets connected to genetic impairments and, consequently, suggest the better definition of cell protection and treatments through accurate molecular profiling that includes membrane lipidomes.
Oral Health Preventive Program in Patients with Autism Spectrum Disorder
Authors: Carli Elisabetta , Pasini Marco , Pardossi Francesca , Capotosti Isabella , Narzisi Antonio , Lardani Lisa
Published: April 2022

The aim of the study was to evaluate clinical hygienic parameters, patient collaboration, and dental habits in patients with ASD (autism spectrum disorder) before and after a tailored prevention program. A total of 100 patients (78 males and 22 females, mean age 8 ± 0.7 years old) was recruited, with ages ranging from 7 to 16 years old, and diagnoses of ASD. We evaluated the plaque index (IP), gingival index (IG), the dmft/DMFT, the frequency of tooth brushing, and the frequency of snacks for each patient. Patient behaviour was evaluated with the Frankl scale, and each patient was individually reassessed after five visits from the first one by the same operator. The t test was used to compare the parameters before and after the inclusion in the dedicated dental pathway. From T1 to T2 we found a significant improvement of the IP (p < 0.001), IG (p < 0.001), and the frequency of tooth brushing (p < 0.001). Concerning the frequency of snacks and the parameter dmft/DMFT, the differences in the observed averages were not significant (p > 0.05). The difference in collaboration between T1 and T2 evaluated by the Frankl scale was statistically significant (p < 0.001). It was found that the prevention program allowed a significant improvement in both clinical parameters and patient behaviour. The personalized digital supports can have a key role for success in familiarization and desensitization processes of patients affected by ASD, leading an increase in their collaboration.
Characterization of the Impacts of Living at High Altitude in Taif: Oxidative Stress Biomarker Alterations and Immunohistochemical Changes
Authors: Soliman Mohamed Mohamed , Aldhahrani Adil , Althobaiti Fayez , Ahmed Mohamed Mohamed , Sayed Samy , Alotaibi Saqer , Shukry Mustafa , El-Shehawi Ahmed M.
Published: April 2022

At high elevations, the human body experiences a number of pathological, physiological, and biochemical changes, all of which have adverse impacts on human health and organ vitality. This study aimed to investigate the alterations in the liver and kidney biomarkers, oxidative stress markers, gene expression, and cellular histology of rats maintained at high altitudes and normal sea level. A total of twenty male Wistar rats at 2 months of age were randomly assigned to two groups. The rats in group A were maintained at normal sea level in Jeddah, whereas rats in group B were maintained in an area in Taif 2600 m above sea level. After 2 months of housing, orbital blood samples were collected for the analysis of significant biochemical indicators of oxidative stress biomarkers of the liver and kidneys. Liver and kidney tissues from both groups were taken to examine the hepatorenal changes occurring at the biochemical, histological, immunohistochemical, and genetic levels. The results revealed substantial increases in the serum levels of liver and kidney biomarkers (GPT, GOT, urea, and creatinine) and decreases in the serum levels of antioxidant biomarkers (SOD, catalase, GSH, and NO). In parallel, the levels of the malondialdehyde (MDA) tissue damage marker and inflammatory cytokines (IL-1β, TNF-α, and IFN-γ) were increased in the high-altitude group compared to the normal sea level group. In addition, there were significant alterations in the oxidative and inflammatory status of rats that lived at high altitude, with considerable upregulation in the expression of hepatic VEGF, type 1 collagen, Cox-2, TNF-α, and iNOS as well as renal EPASI, CMYC, HIF-α, and EGLN-2 genes in the high-altitude group compared with controls housed at normal sea level. In conclusion, living at high altitude induces hepatorenal damage and biochemical and molecular alterations, all of which may serve as critical factors that must be taken into account for organisms living at high altitudes.
Quantifying Interactive Cooling Effects of Morphological Parameters and Vegetation-Related Landscape Features during an Extreme Heat Event
Authors: Kianmehr Ayda , Lim Theodore C.
Published: April 2022

In this study, we apply the ENVI-met model to evaluate the effects of combinations of morphological and vegetation-related landscape features on urban temperatures and thermal comfort. We simulated the thermal conditions of 126 scenarios, varying the aspect ratios of street canyons, vegetation cover and density, surface materials, and orientations toward the prevalent winds under an extreme heat situation. Our results show how the effects of physical and vegetation parameters interact and moderate each other. We also demonstrate how sensitive thermal comfort indices such as temperature and relative humidity are to the built environment parameters during different hours of a day. This study’s findings highlight the necessity of prioritizing heat mitigation interventions based on the site’s physical characteristics and landscape features and avoiding generic strategies for all types of urban environments.
Time-Lapse Camera Monitoring and Study of Recurrent Breaching Flow Slides in Cap Ferret, France
Authors: Nédélec Yves , Fouine Philippe , Gayer Cyrille , Collin Florent
Published: April 2022

In this paper, we present a low-cost method designed to monitor recurrent breaching flow slides that impact the security of a beach. This beach, located in France at the inlet of Arcachon Bay, connects a sand spit to a tidal channel while ending at the toe of a coastal defense. Monitoring is based on capturing images and intends to add continuous information to intermittent direct observations so that triggering and influencing factors can be assessed more precisely. The method is based on time-lapse picture collection and processing. The field of view shows successive emerged manifestations of flow slide phenomena, as well as some possibly related environmental elements. On-site application for 576 days provides important indications and details on flow slide event progress and beach recovery. A simple but quantitative analysis of the influence of sand spit topographic changes is proposed as a preliminary approach of the method’s suitability for studies of environmental processes in conjunction with coast protection.
Graphene-Integrated Plasmonic Metamaterial for Manipulation of Multi-Band Absorption, Based on Near-Field Coupled Resonators
Authors: Nam Man Hoai , Tung Bui Son , Khuyen Bui Xuan , Ha Duong Thi , Ngoc Nguyen Van , Tran Manh Cuong , Le Dac Tuyen , Lam Vu Dinh , Chen Liangyao , Zheng Haiyu , Lee YoungPak
Published: April 2022

We demonstrated a multi-band plasmonic metamaterial absorber (MA), based on the near-field coupled resonators. In addition to the individual resonances of resonators in the proposed structure, which were split-ring resonator (SRR) and cross-shape structures, another resonance was also excited owing to the coupling of resonators, revealing a triple-band absorption. Furthermore, to control the absorption behavior, on the top of the SRRs, the identical SRRs made of graphene ink were pasted. By increasing the resistance of graphene ink, the coupling strength was weakened, changing the triple-band absorption to a dual-band one. Our work might be useful as the controllable devices, based on graphene-integrated plasmonic MA, such as filters, detectors and energy harvesters.
Electrophoretic Deposited Quartz Powder-Assisted Growth of Multicrystalline Silicon
Authors: Quan Xiang , Yuan Ningyi , Huang Chunlai , Liao Jilong
Published: April 2022

Ingot multicrystalline silicon (Mc-Si) needs to be improved in quality and reduced in cost compared with Czochralski monocrystalline silicon. A uniform and dense quartz nucleation layer was obtained by the electrophoretic deposition of quartz powder on the surface of the silicon wafer. The deposited silicon wafer was annealed at 600 °C for 1 h, and one side of the silicon wafer with the quartz layer was glued to the crucible. During the growth of Mc-Si crystal, the dense quartz powder can play a nucleation role. The results show that the average lifetime of the minority carriers a of quartz-assisted silicon ingot is 7.4 μs. The overall dislocation density of an electrophoretic deposition quartz-assisted silica ingot is low, and the defect density in the middle of the silica ingot is 1.5%, which is significantly lower than that of spray quartz (3.1%) and silicon particle (4.2%). Moreover, electrophoretic deposited quartz-assisted mc-Si can obtain oriented grains, which offers a potential to apply alkaline texturing on mc-Si wafers.