bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Genetics and Cytotoxic Responses to Titanium Diboride and Zinc Borate Nanoparticles on Cultured Human Primary Alveolar Epithelial Cells
Authors: Türkez Hasan , Arslan Mehmet Enes , Tatar Arzu , Özdemir Özlem , Sönmez Erdal , Çadirci Kenan , Hacimüftüoğlu Ahmet , Ceylan Bahattin , Açikyildiz Metin , Kahraman Cigdem Yuce , Geyikoğlu Fatime , Tatar Abdulgani , Mardinoglu Adil
Published: March 2022

Titanium diboride (TiB2) and zinc borate (Zn3BO6) have been utilized in wide spectrum industrial areas because of their favorable properties such as a high melting point, good wear resistance, high hardness and thermal conductivity. On the other hand, the biomedical potentials of TiB2 and Zn3BO6 are still unknown because there is no comprehensive analysis that uncovers their biocompatibility features. Thus, the toxicogenomic properties of TiB2 and Zn3BO6 nanoparticles (NPs) were investigated on human primary alveolar epithelial cell cultures (HPAEpiC) by using different cell viability assays and microarray analyses. Protein-Protein Interaction Networks Functional Enrichment Analysis (STRING) was used to associate differentially expressed gene probes. According to the results, up to 10 mg/L concentration of TiB2 and Zn3BO6 NPs application did not stimulate a cytotoxic effect on the HPAEpiC cell cultures. Microarray analysis revealed that TiB2 NPs exposure enhances cellular adhesion molecules, proteases and carrier protein expression. Furthermore, Zn3BO6 NPs caused differential gene expressions in the cell cycle, cell division and extracellular matrix regulators. Finally, STRING analyses put forth that inflammation, cell regeneration and tissue repair-related gene interactions were affected by TiB2 NPs application. Zn3BO6 NPs exposure significantly altered inflammation, lipid metabolism and infection response activator-related gene interactions. These investigations illustrated that TiB2 and Zn3BO6 NPs exposure may affect different aspects of cellular machineries such as immunogenic responses, tissue regeneration and cell survival. Thus, these types of cellular mechanisms should be taken into account before the use of the related NPs in further biomedical applications.
Advanced Estimation of Compressive Strength and Fracture Behavior in Ceramic Honeycombs by Polarimetry Measurements of Similar Epoxy Resin Honeycombs
Authors: Köllner David , Tolve-Granier Bastien , Simon Swantje , Kakimoto Ken-ichi , Fey Tobias
Published: March 2022

Finding a non-destructive characterization method for cellular ceramics’ compressive strength and fracture behavior has been a challenge for material scientists for years. However, for transparent materials, internal stresses can be determined by the non-destructive photoelastic measurements. We propose a novel approach to correlate the photoelastic stresses of polymer (epoxy resin) prototypes with the mechanical properties of ceramics (alumina). Regular and inverse epoxy honeycombs were 3D-printed via stereolithography with varying structure angles from −35° to 35°, with negative angles forming an auxetic and positive hexagonal lattice. Photoelastic measurements under mechanical loading revealed regions of excess stress, which directly corresponded to the initial fracture points of the ceramic honeycombs. These honeycombs were made by a combination of 3D printing and transfer molding from alumina. The photoelastic stress distribution was much more homogeneous for angles of a smaller magnitude, which led to highly increased compressive strengths of up to 446 ± 156 MPa at 0°. By adapting the geometric structural model from Gibson and Ashby, we showed that we could use a non-destructive technique to determine the compressive strength of alumina honeycombs from the median photoelastic stress measured on similar epoxy honeycomb structures.
Bacterial Membranes Are More Perturbed by the Asymmetric Versus Symmetric Loading of Amphiphilic Molecules
Authors: Drew Bennett W. F. , Fox Stephen J. , Sun Delin , Maupin C. Mark
Published: March 2022

Characterizing the biophysical properties of bacterial membranes is critical for understanding the protective nature of the microbial envelope, interaction of biological membranes with exogenous materials, and designing new antibacterial agents. Presented here are molecular dynamics simulations for two cationic quaternary ammonium compounds, and the anionic and nonionic form of a fatty acid molecule interacting with a Staphylococcus aureus bacterial inner membrane. The effect of the tested materials on the properties of the model membranes are evaluated with respect to various structural properties such as the lateral pressure profile, lipid tail order parameter, and the bilayer’s electrostatic potential. Conducting asymmetric loading of molecules in only one leaflet, it was observed that anionic and cationic amphiphiles have a large impact on the Staphylococcus aureus membrane’s electrostatic potential and lateral pressure profile as compared to a symmetric distribution. Nonintuitively, we find that the cationic and anionic molecules induce a similar change in the electrostatic potential, which points to the complexity of membrane interfaces, and how asymmetry can induce biophysical consequences. Finally, we link changes in membrane structure to the rate of electroporation for the membranes, and again find a crucial impact of introducing asymmetry to the system. Understanding these physical mechanisms provides critical insights and viable pathways for the rational design of membrane-active molecules, where controlling the localization is key.
The Impact of Operational Parameters on Polypropylene Membrane Performance during the Separation of Oily Saline Wastewaters by the Membrane Distillation Process
Authors: Tomczak Wirginia , Gryta Marek
Published: March 2022

In the present study, membrane distillation (MD) was applied for the treatment of oily saline wastewaters produced on ships sailing the Baltic Sea. For comparison purposes, experiments were also carried out with model NaCl solutions, the Baltic Seawater and oil in water emulsions. The commercial Accurel PP V8/2 membranes (Membrana GmbH, Germany) were used. In order to investigate the impact of the operational parameters on the process performance, the experiments were conducted under various values of the feed flow velocity (from 0.03 to 0.12 m/s) and the feed temperature (from 323 to 343 K). The obtained results highlight the potential of PP membranes application for a stable and reliable long-term treatment of oily wastewater. It was demonstrated that the permeate flux increased significantly with increasing feed temperature. However, the lower temperature ensured the limited scaling phenomenon during the treatment of oily wastewaters. Likewise, increasing the feed flow velocity was beneficial to the increase in the flux. Moreover, it was found that performing a cyclic rinsing of the module with a 3% HCl solution is an effective method to maintain a satisfactory module performance. The present study sheds light on improving the MD for the treatment of oily wastewaters.
Near Net Forming Process Optimization of 17-4PH Steel Based on High Temperature Deformation Behaviour and Microstructure Control
Authors: Guo Yongqiang , Wang Yongjin , Wang Zhengyu , Han Jingtao , Xu Chunguo
Published: March 2022

In this paper, the high temperature deformation behaviour of 17-4PH steel was studied. A new forming optimization method was proposed. The study will help guide the obtainment of high-precision and high-mechanical properties forging products in the aerospace industry. A hot compression test was carried out on a Gleeble1500D test machine (DUFFERS SCIENTFIC, Beijing, China). The stress–strain behaviour at a temperature of 1000 °C~1150 °C and strain rate of 0.1~10 s−1 was analysed. Through the analysis of multiple heating tests, we come to the conclusion that the heating times should be controlled within 1 or 2 times in order to obtain a grain size above grade 6. Optimization analysis of the forming process was carried out to shorten the process and ensure the precision of the product. The preform shape was obtained quickly by fourth-order curve fitting. According to the method of finite element numerical simulation, the near net forming process within two heating procedures was determined. A 17-4PH forging product with precise shape and qualified structure was obtained through actual forming test.
Predictive Modeling of Blast Furnace Gas Utilization Rate Using Different Data Pre-Processing Methods
Authors: Jiang Dewen , Wang Zhenyang , Li Kejiang , Zhang Jianliang , Ju Le , Hao Liangyuan
Published: March 2022

The gas utilization rate (GUR) is an important indicator parameter for reflecting the energy consumption and smooth operation of a blast furnace (BF). In this study, the original data of a BF are pre-processed by two methods, i.e., box plot and 3σ criterion, and two data sets are obtained. Then, support vector regression (SVR) is used to construct a prediction model based on the two data sets, respectively. The state parameters of a BF are selected as input parameters of the model. Gas utilization after one hour (GUR-1h), two hours (GUR-2h), and three hours (GUR-3h) are selected as output parameters, respectively. The simulation result demonstrates that using the 3σ criterion to pre-process the raw data leads to better prediction of the model compared to using the box plot. Moreover, the model has the best predictive effect when the output parameter is selected as GUR-1h.
Cooling Rate Controlled Aging of a Co-Free Fe-Ni-Cr-Mo-Ti-Al Maraging Steel
Authors: Zeisl Stefan , Schnitzer Ronald
Published: March 2022

Maraging steels are high-strength steels that are hardened by the formation of precipitates during an isothermal aging heat treatment. Depending on the aging temperature and time the cooling rate after holding can play a significant factor on the development of the microstructure and mechanical properties. This study seeks to show how the cooling time influences the precipitation hardening effect, austenite reversion and the development of hardness and impact toughness. The material was aged at a constant temperature using holding times of 0 h, 4 h and 15 h and cooled with different cooling rates resulting in cooling times of 7 h, 28 h and 56 h. The microstructure was characterized using a combination of electron backscatter diffraction, X-ray diffraction and atom probe tomography with cluster-based precipitate analysis. It is shown that the effect of the cooling time is strongly dependent on the holding time and that a longer cooling time can improve hardness and impact toughness.
Network Reconstruction and Modelling Made Reproducible with moped
Authors: Saadat Nima P. , van Aalst Marvin , Ebenhöh Oliver
Published: March 2022

Mathematical modeling of metabolic networks is a powerful approach to investigate the underlying principles of metabolism and growth. Such approaches include, among others, differential-equation-based modeling of metabolic systems, constraint-based modeling and metabolic network expansion of metabolic networks. Most of these methods are well established and are implemented in numerous software packages, but these are scattered between different programming languages, packages and syntaxes. This complicates establishing straight forward pipelines integrating model construction and simulation. We present a Python package moped that serves as an integrative hub for reproducible construction, modification, curation and analysis of metabolic models. moped supports draft reconstruction of models directly from genome/proteome sequences and pathway/genome databases utilizing GPR annotations, providing a completely reproducible model construction and curation process within executable Python scripts. Alternatively, existing models published in SBML format can be easily imported. Models are represented as Python objects, for which a wide spectrum of easy-to-use modification and analysis methods exist. The model structure can be manually altered by adding, removing or modifying reactions, and gap-filling reactions can be found and inspected. This greatly supports the development of draft models, as well as the curation and testing of models. Moreover, moped provides several analysis methods, in particular including the calculation of biosynthetic capacities using metabolic network expansion. The integration with other Python-based tools is facilitated through various model export options. For example, a model can be directly converted into a CobraPy object for constraint-based analyses. moped is a fully documented and expandable Python package. We demonstrate the capability to serve as a hub for integrating reproducible model construction and curation, database import, metabolic network expansion and export for constraint-based analyses.
Intensity Measurements of a Landfalling Tropical Cyclone Using Conventional Coastal Weather Radar
Authors: Yurchak Boris S.
Published: March 2022

Tropical cyclone (TC) intensity observations considerably improve forecast models. They are particularly used to continuously measure TC intensity for landfalling cyclones to improve their forecast. For example, TC Irving, which operated in the Gulf of Tonkin, South China Sea, on 23–24 July 1989, was observed by a conventional weather radar installed at the Phu Lien Observatory in North Vietnam. The maximum wind speed was calculated by the hyperbolic-logarithmic approximation (HLS-approximation) of spiral cloud-rain bands (SCRBs) of recorded TC radar images. The data spanned about 15 h. Ground-based estimates of the cyclone intensity were obtained from pressure measurements at two coastal weather stations. A comparison of these estimates with the HLS wind resulting from the HLS approximation of SCRBs showed satisfactory synchronization. In particular, radar and meteorological data indicated cyclone intensification near landfall and rapid cyclone intensification after landfall. Both intensifications were accompanied by polygonal eye shapes. This study demonstrates the feasibility of using the HLS-approximation technique for retrieving TC intensity variation from conventional weather radar data.
Synthesis-Gas Production from Methane over Ni/CeO2 Catalysts Synthesized by Co-Precipitation Method in Different Solvents
Authors: Asencios Yvan J. O. , Elias Kariny F. M. , de Zawadzki Andressa , Assaf Elisabete M.
Published: March 2022

Ni/CeO2 catalysts were synthesized by the coprecipitation method in a basic medium, using different solvents: water, methanol, ethanol, and isopropanol (Ni content, 10% wt.). These catalysts were tested in the production of syngas through the oxidative reforming of methane (ORM), and partial oxidation of methane (POM). The results of this research demonstrated that the use of alcohols (methanol, ethanol, and isopropanol) during the preparation of the Ni/CeO2 catalysts by the coprecipitation method, improved their characteristics such as crystallite size (nm), surface area (m2·g−1), and reducibility (measured by H2-TPR) that influenced on their catalytic performance in ORM and POM reactions. The best solvent of this study was isopropanol. The use of alcohols (methanol, ethanol, isopropanol) in the co-precipitation method led to the formation of filamentous carbon on the catalyst after the reactions. The catalyst synthesized in the water proved to be inefficient in the POM and ORM reactions and led to the formation of amorphous carbon after the reactions.
Field-Dependent Rheological Properties of Magnetorheological Elastomer with Fountain-Like Particle Chain Alignment
Authors: Fakhree Muhammad Akif Muhammad , Nordin Nur Azmah , Nazmi Nurhazimah , Mazlan Saiful Amri , Aziz Siti Aishah Abdul , Ubaidillah Ubaidillah , Ahmad Fauzan , Choi Seung-Bok
Published: March 2022

Magnetorheological elastomer (MRE) consists of magnetic particles known as carbonyl iron (CIPs), which have been locked in a silicone-based matrix, in various alignments. However, current MRE exhibits inadequate rheological properties due to several issues such as particle alignments. Therefore, in this study, a new approach of the particle alignment of CIPs in MRE, namely fountain-like structure, is introduced. It is expected that this kind of MRE exhibits enhancement rheological responses, in off- and on-state conditions. This work includes the development of a new mold that can produce various directions of magnetic flux lines in order to have fountain-like structures of CIPs in MRE, during the curing process. Three types of particle alignments in MRE, namely isotropic, fountain-like and inverted fountain-like, are fabricated. The rheological properties of MRE in terms of storage modulus and MR effect are measured in an oscillatory shear mode using a rheometer. The results have revealed that fountain-like MRE exhibits higher storage modulus than the isotropic MRE, approximately 0.6 MPa of increment in the strain sweep test, in an on-state condition. Furthermore, it has been demonstrated from strain, frequency and the current sweep test that the rheological properties of fountain-like MRE related to storage modulus and magnetorheological (MR) effect are higher compared to the inverted fountain-like MRE.
Synthetic Cathinones: Recent Developments, Enantioselectivity Studies and Enantioseparation Methods
Authors: Almeida Ana Sofia , Silva Bárbara , Pinho Paula Guedes de , Remião Fernando , Fernandes Carla
Published: March 2022

New psychoactive substances represent a public health threat since they are not controlled by international conventions, are easily accessible online and are sold as a legal alternative to illicit drugs. Among them, synthetic cathinones are widely abused due to their stimulant and hallucinogenic effects. To circumvent the law, new derivatives are clandestinely synthesized and, therefore, synthetic cathinones keep emerging on the drug market, with their chemical and toxicological properties still unknown. In this review, a literature assessment about synthetic cathinones is presented focusing on the recent developments, which include more than 50 derivatives since 2014. A summary of their toxicokinetic and toxicodynamic properties are also presented. Furthermore, synthetic cathinones are chiral compounds, meaning that they can exist as two enantiomeric forms which may present different biological and toxicological activities. To analyze the enantiomers, the development of enantiomeric resolution methods for synthetic cathinones is crucial. Many methods have been reported over the years that include mostly chromatographic and electromigration techniques, with liquid chromatography using chiral stationary phases being the technique of choice. This review intended to present an overview of enantioselectivity studies and enantioseparation analysis regarding synthetic cathinones, highlighting the relevance of chirality and current trends.
Self-Powered Organometal Halide Perovskite Photodetector with Embedded Silver Nanowires
Authors: Beisenbayev Almaz R. , Sadirkhanov Zhandos T. , Yerlanuly Yerassyl , Kaikanov Marat I. , Jumabekov Askhat N.
Published: March 2022

Metal–semiconductor–metal (MSM) configuration of perovskite photodetectors (PPDs) suggests easy and low-cost manufacturing. However, the basic structures of MSM PPDs include vertical and lateral configurations, which require the use of expensive materials such as transparent conductive oxides or/and sophisticated fabrication techniques such as lithography. Integrating metallic nanowire-based electrodes into the perovskite photo-absorber layer to form one-half of the MSM PPD structure could potentially resolve the key issues of both configurations. Here, a manufacturing of solution-processed and self-powered MSM PPDs with embedded silver nanowire electrodes is demonstrated. The embedding of silver nanowire electrode into the perovskite layer is achieved by treating the silver nanowire/perovskite double layer with a methylamine gas vapor. The evaporated gold layer is used as the second electrode to form MSM PPDs. The prepared MSM PPDs show a photoresponsivity of 4 × 10−5 AW−1 in the UV region and 2 × 10−5 AW−1 in the visible region. On average, the devices exhibit a photocurrent of 1.1 × 10−6 A under white light (75 mW cm−2) illumination with an ON/OFF ratio of 83.4. The results presented in this work open up a new method for development and fabrication of simple, solution-processable MSM self-powered PPDs.
Nano Geochemistry
Authors: Schäfer Thorsten , Lee Woojin , Darbha Gopala
Published: March 2022

It is our great pleasure to briefly introduce our motivation to collect scientific contributions for this Special Issue, entitled “Nano Geochemistry” [...]
Assessment of Systemic Toxicity, Genotoxicity, and Early Phase Hepatocarcinogenicity of Iron (III)-Tannic Acid Nanoparticles in Rats
Authors: Hlaing Chi Be , Chariyakornkul Arpamas , Pilapong Chalermchai , Punvittayagul Charatda , Srichairatanakool Somdet , Wongpoomchai Rawiwan
Published: March 2022

Iron-tannic acid nanoparticles (Fe-TA NPs) presented MRI contrast enhancement in both liver cancer cells and preneoplastic rat livers, while also exhibiting an anti-proliferative effect via enhanced autophagic death of liver cancer cells. Hence, a toxicity assessment of Fe-TA NPs was carried out in the present study. Acute and systemic toxicity of intraperitoneal Fe-TA NPs administration was investigated via a single dose of 55 mg/kg body weight (bw). Doses were then repeated 10 times within a range of 0.22 to 5.5 mg/kg bw every 3 days in rats. Furthermore, clastogenicity was assessed by rat liver micronucleus assay. Carcinogenicity was evaluated by medium-term carcinogenicity assay using glutathione S-transferase placental form positive foci as a preneoplastic marker, while three doses ranging from 0.55 to 17.5 mg/kg bw were administered 10 times weekly via intraperitoneum. Our study found that the LD50 value of Fe-TA NPs was greater than 55 mg/kg bw. Repeated dose administration of Fe-TA NPs over a period of 28 days and 10 weeks revealed no obvious signs of systemic toxicity, clastogenicity, and hepatocarcinogenicity. Furthermore, Fe-TA NPs did not alter liver function or serum iron status, however, increased liver iron content at certain dose in rats. Notably, antioxidant response was observed when a dose of 17.5 mg/kg bw was given to rats. Accordingly, our study found no signs of toxicity, genotoxicity, and early phase hepatocarcinogenicity of Fe-TA NPs in rats.
Combination of Texture-Induced Oral Processing and Vegetable Preload Strategy Reduced Glycemic Excursion but Decreased Insulin Sensitivity
Authors: Wu Yixue , Fan Zhihong , Lou Xinling , Zhao Wenqi , Lu Xuejiao , Hu Jiahui , Han Yue , Liu Anshu
Published: March 2022

This study aimed to investigate the effect of the oral processing of vegetables induced by texture modification on acute postprandial glycemic response (GR) and insulin response (IR) when co-ingested and ingested prior to a rice meal. In a randomized crossover trial, 14 healthy female subjects consumed (1) co-ingestion of soft broccoli and rice (SR); (2) co-ingestion of hard broccoli and rice (HR); (3) soft broccoli prior to rice (S+R); (4) hard broccoli prior to rice (H+R); (5) rice (R). Postprandial GR and IR was compared between test meals over a period of 180-min, and the oral processing behaviors were measured for each test food samples. Hard broccoli was observed to have a higher mastication time and chews than soft broccoli. All the broccoli meals resulted in reduced incremental peak glucose (IPG) and an increased incremental area under the insulin curve in 180 min (iAUC0–180) compared with R. The S+R curbed the IPG by 40% with comparable HOMA-IR AUC0–180 compared with R, while the H+R elevated the HOMA-IR AUC0–180 by 62% more than that of R. In conclusion, the soft broccoli intake prior to a rice meal effectively attenuated postprandial GR, without lowering insulin sensitivity as its hard counterpart did.
Maternal Obesity in Twin Pregnancy: The Role of Nutrition to Reduce Maternal and Fetal Complications
Authors: de la Calle María , Bartha Jose L. , Marín Clara , Rus Juan Carlos , Córcoles Guillermo , Ruvira Santiago , Ramiro-Cortijo David
Published: March 2022

There are more and more obese mothers with twin gestations. For a long time before, the responses of lymphocytes and platelets in obese women can cause a low-grade inflammation. In addition, a proper control of gestational weight gain would improve the outcomes in mothers with high pre-gestational body mass index (BMI). In women with high pre-gestational BMI and twin pregnancy, our aims were to explore the biochemical and hematological parameters and to study the rate of obstetric adverse outcomes. This was an observational and retrospective study conducted in the Hospital Universitario La Paz (Madrid, Spain). We included 20 twin pregnancies as the lean group (BMI = 18.5–24.9 kg/m2), homogeneous in the maternal age and ethnicity, and having parity with other 20 twin pregnancies as the obese group (BMI ≥ 30 kg/m2). The maternal data and maternal, fetal, obstetric, and neonatal complications were collected from the medical records. In the first and third trimester of pregnancy, the biochemical and hematological parameters of the blood were assayed. In this cohort, gestational weight gain was significantly lower in the obese than lean group. In the first trimester, the hemoglobin levels in obese women (12.1 ± 0.8 g/dL) were lower than lean women (12.6 ± 0.7 g/dL; p-Value = 0.048). In addition, the tendency of glucose levels, TSH levels and platelets was to increase in obese compared to lean women. In the third trimester, the TSH levels were higher in obese (3.30 ± 1.60 mUI/L) than lean women (1.70 ± 1.00 mUI/L; p-Value = 0.009). Furthermore, there was a tendency for levels of platelets and lymphocytes to increase in obese compared to lean women. No significant differences were detected in the rate of maternal, fetal, obstetrical, and neonatal complications between the groups. The hemoglobin, platelets, lymphocytes and TSH levels need further investigation to understand potential subclinical inflammation in obese women. Furthermore, obese women with twin pregnancies should follow-up with a specialist nutritionist, to help them control their gestational weight gain with appropriate dietary measures.
Stop the Pop: A Mixed-Methods Study Examining Children’s Physical and Emotional Responses during Three Days of Sugary Drink Cessation
Authors: Kaidbey Jasmine H. , Ferguson Kacey , Halberg Sabrina E. , Racke Caroline , Visek Amanda J. , Gearhardt Ashley N. , Juliano Laura M. , Dietz William H. , Sacheck Jennifer , Sylvetsky Allison C.
Published: March 2022

Despite public health efforts to reduce sugary drink consumption, children’s intake continues to exceed recommendations. While numerous barriers to lowering sugary drink consumption have been identified, aversive feelings during sugary drink cessation may further challenge sustained reduction in children’s sugary drink consumption. Herein, we describe “Stop the Pop”, an intervention to examine children’s physical and emotional responses during three days of sugary drink cessation. Children (n = 150) ages 8–14, who reported habitual consumption of ≥12 ounces of sugary drinks daily, were instructed to avoid sweetened beverages for three days. At baseline and on each day of cessation, children completed a daily feelings questionnaire, and a subset of children (n = 30) also completed a qualitative interview following cessation. During sugary drink cessation, children reported physical and emotional improvements, including being less tired, angry, and annoyed; having less trouble sleeping; and less frequently arguing with others, getting in trouble, and getting mad. However, unfavorable responses, such as mood disturbances and having less energy, were reported by some participants. Our results suggest that children who habitually consume sugary drinks may experience physical and emotional improvements during short-term sugary drink cessation, although longer-term examination is needed and inter-individual variability in responses to cessation warrants further study.
Non-Destructive Identification of Drugs in Plastic Packaging Using Attenuated Total Reflection Terahertz Time Domain Spectroscopy
Authors: Hashimoto Kazuma , Tripathi Saroj R.
Published: March 2022

In this study, we demonstrate that drugs in plastic packaging can be identified without being opened using attenuated total reflection terahertz time domain spectroscopy. In this system, the terahertz wave undergoes total internal reflection at the interface between prism and sample, producing an evanescent wave at the interface. The penetration depth of the evanescent waves is larger than the thickness of typical plastic packaging in the sub-terahertz frequency region; therefore, it becomes possible to detect the sample without opening the package. Here, we show that some saccharides samples such as lactose in plastic packaging can be identified using its spectral fingerprint by placing the packaged lactose on the prism. This method has the potential to be used in the non-destructive testing and analysis of a wide variety of samples, such as medicine sachets, to reduce medication dispensing errors in pharmacies.
Lineage Differentiation Potential of Different Sources of Mesenchymal Stem Cells for Osteoarthritis Knee
Authors: Prajwal Gollahalli , Jeyaraman Naveen , Kanth V Krishna , Jeyaraman Madhan , Muthu Sathish , Rajendran Sree , Rajendran Ramya , Khanna Manish , Oh Eun , Choi Kang , Chung Ho , Ahn Byeong-Cheol , Gangadaran Prakash
Published: March 2022

Tissue engineering and regenerative medicine (TERM) have paved a way for treating musculoskeletal diseases in a minimally invasive manner. The regenerative medicine cocktail involves the usage of mesenchymal stem/stromal cells (MSCs), either uncultured or culture-expanded cells along with growth factors, cytokines, exosomes, and secretomes to provide a better regenerative milieu in degenerative diseases. The successful regeneration of cartilage depends on the selection of the appropriate source of MSCs, the quality, quantity, and frequency of MSCs to be injected, and the selection of the patient at an appropriate stage of the disease. However, confirmation on the most favorable source of MSCs remains uncertain to clinicians. The lack of knowledge in the current cellular treatment is uncertain in terms of how beneficial MSCs are in the long-term or short-term (resolution of pain) and improved quality of life. Whether MSCs treatments have any superiority, exists due to sources of MSCs utilized in their potential to objectively regenerate the cartilage at the target area. Many questions on source and condition remain unanswered. Hence, in this review, we discuss the lineage differentiation potentials of various sources of MSCs used in the management of knee osteoarthritis and emphasize the role of tissue engineering in cartilage regeneration.
Daphne jejudoensis Attenuates LPS-Induced Inflammation by Inhibiting TNF-α, IL-1β, IL-6, iNOS, and COX-2 Expression in Periodontal Ligament Cells
Authors: Bae Ji-Yeong , Lee Dong-Seol , Cho You Kyoung , Lee Ji-Yeon , Park Joo-Hwang , Lee Sang Ho
Published: March 2022

Periodontitis is a common disease involving inflammation and tissue destruction in the periodontal region. Although uncontrolled long-term inflammation in the gingiva may lead to loss of the periodontal ligament, treatments or preventive solutions for periodontitis are scarce. The aim of this study is to find anti-inflammatory material from a natural source that can be used to treat or protect against periodontitis. Daphne species (Thymelaeaceae) are important and popular components of traditional Chinese medicine and are used as anti-inflammatory agents. Daphne jejudoensis is an endemic plant that grows on Jeju Island and was identified as a new species in 2013. In this study, for the first time, we investigated the anti-inflammatory effect of D. jejudoensis leaf extract (DJLE) on human periodontal ligament cells. The gene expression levels of pro-inflammatory cytokines (interleukin-1β and 6 and tumor necrosis factor-α) and inflammation-inducible enzymes (inducible nitric oxide synthase and cyclooxygenase-2) were reduced after DJLE treatment with/without lipopolysaccharide stimulation. The findings of this study indicate that D. jejudoensis possesses anti-inflammatory activities, suggesting that DJLE may be a potential preventive and therapeutic agent for periodontitis.
Solid Dispersion Formulations by FDM 3D Printing—A Review
Authors: Khalid Garba M. , Billa Nashiru
Published: March 2022

Additive manufacturing (AM) is revolutionizing the way medicines are designed, manufactured, and utilized. Perhaps, AM appears to be ideal for the fit-for-purpose manufacturing of medicines in contrast to the several disadvantages associated with the conventional fit-for-all mass production that accounts for less than 50% of pharmacotherapeutic treatment/management of diseases especially among children and elderly patients, as well as patients with special needs. In this review, we discuss the current trends in the application of additive manufacturing to prepare personalized dosage forms on-demand focusing the attention on the relevance of coupling solid dispersion with FDM 3D printing. Combining the two technologies could offer many advantages such as to improve the solubility, dissolution, and oral bioavailability of poorly soluble drugs in tandem with the concept of precision medicine and personalized dosing and to address the dilemma of commercial availability of FDM filaments loaded with Class II and/or Class IV drugs. However, thermal treatment especially for heat-sensitive drugs, regulatory, and ethical obligations in terms of quality control and quality assurance remain points of concern. Hence, a concerted effort is needed between the scientific community, the pharmaceutical industries, the regulatory agencies, the clinicians and clinical pharmacists, and the end-users to address these concerns.
Medicinal Chrysanthemum Detection under Complex Environments Using the MC-LCNN Model
Authors: Qi Chao , Chang Jiangxue , Zhang Jiayu , Zuo Yi , Ben Zongyou , Chen Kunjie
Published: March 2022

Medicinal chrysanthemum detection is one of the desirable tasks of selective chrysanthemum harvesting robots. However, it is challenging to achieve accurate detection in real time under complex unstructured field environments. In this context, we propose a novel lightweight convolutional neural network for medicinal chrysanthemum detection (MC-LCNN). First, in the backbone and neck components, we employed the proposed residual structures MC-ResNetv1 and MC-ResNetv2 as the main network and embedded the custom feature extraction module and feature fusion module to guide the gradient flow. Moreover, across the network, we used a custom loss function to improve the precision of the proposed model. The results showed that under the NVIDIA Tesla V100 GPU environment, the inference speed could reach 109.28 FPS per image (416 × 416), and the detection precision (AP50) could reach 93.06%. Not only that, we embedded the MC-LCNN model into the edge computing device NVIDIA Jetson TX2 for real-time object detection, adopting a CPU–GPU multithreaded pipeline design to improve the inference speed by 2FPS. This model could be further developed into a perception system for selective harvesting chrysanthemum robots in the future.
Phylogeographical Analyses of a Relict Fern of Palaeotropical Flora (Vandenboschia speciosa): Distribution and Diversity Model in Relation to the Geological and Climate Events of the Late Miocene and Early Pliocene
Authors: Ben-Menni Schuler Samira , Hamza Hammadi , Blanca Gabriel , Romero-García Ana Teresa , Suárez-Santiago Víctor N.
Published: March 2022

Fern phylogeographic studies have mostly focused on the influence of the Pleistocene climate on fern distributions and the prevalence of long-distance dispersal. The effect of pre-Pleistocene events on the distributions of fern species is largely unexplored. Here, we elucidate a hypothetical scenario for the evolutionary history of Vandenboschia speciosa, hypothesised to be of Tertiary palaeotropical flora with a peculiar perennial gametophyte. We sequenced 40 populations across the species range in one plastid region and two variants of the nuclear gapCp gene and conducted time-calibrated phylogenetic, phylogeographical, and species distribution modelling analyses. Vandenboschia speciosa is an allopolyploid and had a Tertiary origin. Late Miocene aridification possibly caused the long persistence in independent refugia on the Eurosiberian Atlantic and Mediterranean coasts, with the independent evolution of gene pools resulting in two evolutionary units. The Cantabrian Cornice, a major refugium, could also be a secondary contact zone during Quaternary glacial cycles. Central European populations resulted from multiple post-glacial, long-distance dispersals. Vandenboschia speciosa reached Macaronesia during the Pliocene–Pleistocene, with a phylogeographical link between the Canary Islands, Madeira, and southern Iberia, and between the Azores and northwestern Europe. Our results support the idea that the geological and climate events of the Late Miocene/Early Pliocene shifted Tertiary fern distribution patterns in Europe.
The Grapevine Microbiome to the Rescue: Implications for the Biocontrol of Trunk Diseases
Authors: Cobos Rebeca , Ibañez Ana , Diez-Galán Alba , Calvo-Peña Carla , Ghoreshizadeh Seyedehtannaz , Coque Juan José R.
Published: March 2022

Grapevine trunk diseases (GTDs) are one of the most devastating pathologies that threaten the survival and profitability of vineyards around the world. Progressive banning of chemical pesticides and their withdrawal from the market has increased interest in the development of effective biocontrol agents (BCAs) for GTD treatment. In recent years, considerable progress has been made regarding the characterization of the grapevine microbiome, including the aerial part microbiome (flowers, berries and leaves), the wood microbiome, the root environment and vineyard soil microbiomes. In this work, we review these advances especially in relation to the etiology and the understanding of the composition of microbial populations in plants affected by GTDs. We also discuss how the grapevine microbiome is becoming a source for the isolation and characterization of new, more promising BCAs that, in the near future, could become effective tools for controlling these pathologies.
Growth of Deciduous and Evergreen Species in Two Contrasting Temperate Forest Stands in Korea: An Intersite Experiment
Authors: Park Byung Bae , Ko Youngtak , Hernandez Jonathan O. , Byambadorj Ser-Oddamba , Han Si Ho
Published: March 2022

Poor seedling establishment and growth can be a result of the limitation of light and soil resources in the forest understory. Here, we investigate the interacting effects of stand and soil characteristics on the seedling growth of deciduous species (Fraxinus rhynchophylla and Zelkova serrata) and evergreen species (Pinus koraiensis) through a 3-year intersite experiment in two contrasting forest stands. Seedlings were grown in both oak and pine stands using two different soil types, i.e., gray-brown forest soil (GB) and red-yellow forest soil (RY). Soil physicochemical properties, light intensity, tree-seedling height, root-collar diameter (RCD), and biomass growth were analyzed between two stands and/or soil types. Light availability was generally more abundant in the pine stand (mean: 1074.08 lx or 20.25%) than the oak stand (mean: 424.33 lx or 9.20%) throughout the year. The height and RCD growth of fast-growing and deciduous F. rhynchophylla and Z. serrata were higher in the pine than in the oak stand, particularly in GB soil. The growth of the slow-growing and evergreen P. koraiensis was not affected by the forest stand, except for its higher root growth in the oak stand and RY soil. Therefore, abundant light availability can enhance the growth and seedling establishment of F. rhynchophylla and Z. serrata in the pine-stand understory. Contrarily, P. koraiensis may be planted in the understory regardless of light condition, but with a slower growth rate.
The Delay of Raphanus raphanistrum subsp. sativus (L.) Domin Seed Germination Induced by Coumarin Is Mediated by a Lower Ability to Sustain the Energetic Metabolism
Authors: Araniti Fabrizio , Prinsi Bhakti , Espen Luca
Published: March 2022

In the present study, the mode of action of coumarin using the germination process as a target was investigated. A dose–response curve, built using a range of concentrations from 0 to 800 µM, allowed us to identify a key concentration (400 µM) inhibiting the germination process, reducing its speed without compromising seed development. Successively, short time-course (0–48 h) experiments were carried out to evaluate the biochemical and metabolic processes involved in coumarin-induced germination delay. The results pointed out that coumarin delayed K+, Ca2+, and Mg2+ reabsorption, suggesting a late membrane reorganisation. Similarly, seed respiration was inhibited during the first 24 h but recovered after 48 h. Those results agreed with ATP levels, which followed the same trend. In addition, the untargeted metabolomic analysis allowed to identify, among the pathways significantly impacted by the treatment, amino acids metabolism, the TCA cycle, and the glyoxylate pathway. The results highlighted that coumarin was able to interact with membranes reorganisation, delaying them and reducing the production of ATP, as also supported by pathway analysis and cell respiration. The in vivo 31P-NMR analysis supported the hypothesis that the concentration chosen was able to affect plant metabolism, maintaining, on the other hand, its viability, which is extremely important for studying natural compounds’ mode of action.
Polymer Dynamics: Bulk and Nanoconfined Polymers
Authors: Sasaki Takashi
Published: March 2022

The dynamics in polymeric systems affect various important properties including mechanical and thermal behaviors, and extensive studies in this field have been executed not only academically but also practically [...]
Effect of Antioxidants on Thermo-Oxidative Stability and Aging of Bio-Based PA56T and Fast Characterization of Anti-Oxidation Performance
Authors: Xu Qiang , Guan Bing , Guo Weihong , Liu Xiucai
Published: March 2022

Bio-based polyamide 56T (PA56T) is a new type of bio-based polyamide regarded as a promising material for sustainable solutions. The stabilization of PA56T compounded with Irganox 1098, Doverphos S9228, or SH3368 was studied by using a rotational rheometer and a circulating air oven at 150 °C. The thermal-oxidative aging resulted in an increase of the yellow color index of the PA56T/GF composites, which due to the carbonyl group as a chromophore group, continuously formatted during the aging process. After 10 days of aging, the mechanical properties and dynamic mechanical properties increase due to the molecular cross-linking and annealing effects. When the aging time is beyond 20 days, the degradation of molecular chain segments dominates, and the mechanical properties of PA56T/GF deteriorate continuously. The addition of antioxidants only slowed this effect and did not change the process of thermal-oxidative aging, which destroys the molecular chain. The results from both methods are consistent after a series of characterizations by FTIR, XRD, and so on. In the case of samples without lubricant, the rotational rheometer has the benefit of being less time-consuming than the accelerated aging experiment.
A Novel Aminomethacrylate-Based Copolymer for Solubility Enhancement—From Radical Polymer Synthesis to Manufacture and Characterization of Amorphous Solid Dispersions
Authors: Schmied Fabian-Pascal , Bernhardt Alexander , Moers Christian , Meier Christian , Endres Thomas , Klein Sandra
Published: March 2022

The present study covers the synthesis, purification and evaluation of a novel aminomethacrylate-based copolymer in terms of its suitability for improving the solubility and in vitro release of poorly water-soluble drug compounds. The new copolymer was synthesized by solvent polymerization with radical initiation and by use of a chain transfer agent. Based on its composition, it can be considered as a modified type of dimethylaminoethyl methacrylate-butyl methacrylate-methyl methacrylate “EUDRAGIT® E PO” (ModE). ModE was specifically developed to provide a copolymer with processing and application properties that exceed those of commercially available (co-)polymers in solubility enhancement technologies where possible. By varying the concentration of the chain transfer agent in the radical polymerization process, the molecular weight of ModE was varied in a range of 173–305 kDa. To evaluate the solubility-enhancing properties of ModE, a series of drug-loaded extrudates were prepared by hot melt extrusion using the novel—as well as several commercially available—(co-)polymers. These extrudates were then subjected to comparative tests for amorphousness, solubility-enhancing properties, storage stability, and drug release. Celecoxib, efavirenz, and fenofibrate were used as model drugs in all experiments. Of all the (co-)polymers included in the study, ModE with a molecular weight of 173 kDa showed the best performance in terms of desired properties and was shown to be particularly suitable for preparing amorphous solid dispersions (ASDs) of the three model drugs, which in a first set of dissolution experiments showed better release behavior under pH conditions of the fasting stomach than higher molecular weight ModE types, as well as a variety of commercially available (co-)polymers. Therefore, the results demonstrate the successful synthesis of a new copolymer, which in future studies will be investigated in more detail for universal application in the field of solubility enhancement.
Photo-Induced Drug Release from Polymeric Micelles and Liposomes: Phototriggering Mechanisms in Drug Delivery Systems
Authors: Salkho Najla M. , Awad Nahid S. , Pitt William G. , Husseini Ghaleb A.
Published: March 2022

Chemotherapeutic drugs are highly effective in treating cancer. However, the side effects associated with this treatment lower the quality of life of cancer patients. Smart nanocarriers are able to encapsulate these drugs to deliver them to tumors while reducing their contact with the healthy cells and the subsequent side effects. Upon reaching their target, the release of the encapsulated drugs should be carefully controlled to achieve therapeutic levels at the required time. Light is one of the promising triggering mechanisms used as external stimuli to trigger drug release from the light-responsive nanocarriers. Photo-induced drug release can be achieved at a wide range of wavelengths: UV, visible, and NIR depending on many factors. In this review, photo-induced release mechanisms were summarized, focusing on liposomes and micelles. In general, light-triggering mechanisms are based on one of the following: changing the hydrophobicity of a nanocarrier constituent(s) to make it more soluble, introducing local defects within a nanocarrier (by conformational transformation or photo-cleavage of its lipids/polymers chains) to make it more porous or concentrating heat for thermo-sensitive nanocarriers to release their payload. Several research studies were also presented to explore the potentials and limitations of this promising drug release triggering mechanism.