bluuebunny/crossref_metadata_2025 huggingface dataset:
Florida Wetlands: Extension Web Site
Authors: Clark Mark
Published: April 2021

Wetlands cover almost 30% of the state of Florida and account for just over 10% of the remaining wetland area in the lower 48 United States. Over the past 200 years Florida has lost an estimated 10 million acres of wetland, about half of the total area thought to exist in the 1780's. Some of these remaining wetlands are well known, like the Florida Everglades, while others may be small and unassuming. All play a vital role in flood protection, water quality and wildlife habitat. The Florida Wetlands Extension web site is designed to provide you with a better understanding of wetlands and wetland-related issues in Florida where these ecosystems are such a prominent feature. This document outlines some of the subjects covered in the site. his document is Fact Sheet SL217, one of a series of the Soil and Water Science Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. Original publication date August 2004. 

https://edis.ifas.ufl.edu/ss437
RELASI AGAMA DAN NEGARA: TELAAH PEMIKIRAN POLITIK SOEKARNO DAN FAZLUR RAHMAN
Authors: Sudarti Sudarti
Published: April 2021

The author in this study wanted to see the similarities and differences in the political thoughts of Soekarno and Fazlur Rahman about the relationship between religion and the state. The type of research used is library research with a descriptive-comparative method. The results showed that Soekarno and Fazlur Rahman had a different paradigm in viewing the relationship between religion and state. Soekarno has a secularistic paradigm that separates religion and state to be implemented in Indonesia, while Fazlur Rahman has an Integralistic paradigm in which religion (Islam) and the state cannot be separated (integrated). However, these two figures agree that the sovereignty of a country is in the hands of the people and do not agree with the theory of God's sovereignty because God has never acted as politically sovereign nor as a maker of laws or laws.

Keywords: Secularistic Paradigm, Integralistic Paradigm, God's Sovereignty.
Covid-19 in Lupus Nephritis
Authors: Putra Ramadhan Ananda , Raveinal  , Fauzar  , Deka Viotra
Published: April 2021

COVID-19 outbreak is currently being concerned for managing patients withimmunological disorders nowadays, including SLE. Lupus is a complexautoimmune disease characterized by the presence of autoantibodies that againstcell nucleus involved many organs in the body. Patients with SLE will increaserisk of severe infection because the intrinsic respond attack with their immunerespond though immunosuppressive drugs consumption, and will potentiallydamage some organs target associated with their disease. Lupus have multipleclinical manifestations with a fluctuating symptom. Patient who come with thesymptom ofbreathlessness will getworse day by day. The symptom could be felt inthe same time as fatigue, joint pain, hair loss, malar rash, oral ulcer, pleuraleffusion and swollen feet. There's a patient with antinuclear antibody positive foranti-smith and anti-Ro/SS-A. She was diagnosed with COVID-19, SLE withnephritis, haemolytic anemia, vasculitis and pleural effusions. The clinicalmanifestations of this patient indicate a COVID-19 with lupus nephritis that hassevere disease. She was being treated with methylprednisolone andhydroxychloroquine for SLE and azithromycin plus oseltamivir as a therapy forCOVID-19. The effect of hydroxychloroquine on SARS-CoV-2 was better seen inpatients with SLE who gotthe medication regularly. Patients went home after 24days of hospitalization after negative RT-PCR results and clinical improvement ofLES.
Determinants of Teacher Effectiveness: Pedagogical and Didactic Training
Authors: Konstantinou Charalambos , Konstantinou Ioannis
Published: April 2021

The purpose of this article is to highlight the factors that decisively affect the effectiveness of the teacher in fulfilling his role, giving special importance to his pedagogical and didactic training. Initially, the role of the teacher is approached in relation to the mission of the school and the characteristics of an effective teacher. Subsequently, the content and the importance of pedagogical and didactic training for the exercise of the educational work are approached, as well as the additional reasons that impose it. In addition, the existing pedagogical and didactic training of current teachers is approached, in relation to the consequences of its absence for the educational process and the student. The article concludes with the final findings and remarks.
Snail-Family Proteins: Role in Carcinogenesis and Prospects for Antitumor Therapy
Authors: Yastrebova Margarita A. , Khamidullina Alvina I. , Tatarskiy Victor V. , Scherbakov Alexander M.
Published: April 2021

The review analyzes Snail family proteins, which are transcription factors involved in the regulation of the epithelial-mesenchymal transition (EMT) of tumor cells. We describe the structure of these proteins, their post-translational modification, and the mechanisms of Snail-dependent regulation of genes. The role of Snail proteins in carcinogenesis, invasion, and metastasis is analyzed. Furthermore, we focus on EMT signaling mechanisms involving Snail proteins. Next, we dissect Snail signaling in hypoxia, a condition that complicates anticancer treatment. Finally, we offer classes of chemical compounds capable of down-regulating the transcriptional activity of Snails. Given the important role of Snail proteins in cancer biology and the potential for pharmacological inhibition, Snail family proteins may be considered promising as therapeutic targets.
Suppression of the Immune Response by Syngeneic Splenocytes Adoptively Transferred to Sublethally Irradiated Mice
Authors: Kalinina Anastasia A. , Khromykh Ludmila M. , Kazansky Dmitry B. , Deikin Alexey V. , Silaeva Yulia Yu.
Published: April 2021

The peripheral T-cell pool consists of several, functionally distinct populations of CD8+ T cells. CD44 and CD62L are among the major surface markers that allow us to define T-cell populations. The expression of these molecules depends on the functional status of a T lymphocyte. Under lymphopenic conditions, peripheral T cells undergo homeostatic proliferation and acquire the memory-like surface phenotype CD44hiCD62Lhi. However, the data on the functional activity of these cells remains controversial. In this paper, we analyzed the effects of the adoptive transfer of syngeneic splenocytes on the recovery of CD8+ T cells in sublethally irradiated mice. Our data demonstrate that under lymphopenia, donor lymphocytes form a population of memory-like CD8+ T cells with the phenotype CD122+CD5+CD49dhiCXCR3+ that shares the phenotypic characteristics of true memory cells and suppressive CD8+ T cells. Ex vivo experiments showed that after adoptive transfer in irradiated mice, T cells lacked the functions of true effector or memory cells; the allogeneic immune response and immune response to pathogens were greatly suppressed in these mice.
A Study of Vegetation Geomorphology Relationship along the sections of Balasore and Kanthi Coastal Plains, India
Authors: Paul Anurupa , Debnath Asim , Sardar Joydeb , Bandyopadhyay Jatisankar
Published: April 2021

Coastal geomorphology deals with the various landforms and different processes involved in the development of coast. In the alluvium coast, biology plays a significant role as a coastal process. The present study aims at the role of vegetation in the geomorphic system, with the main consideration of flora. The study has been conducted in an alluvium coast along the Bay of Bengal adjacent to the sections of Balasore and Kanthi coastal plain. The geomorphological map and Normalized Difference Vegetation Index (NDVI) have been generated with the help of Landsat 5 TM, Landsat 8 OLI & SOI Toposheet with the help of Arc GIS & Erdas Imagine software. The ground truthing of relationships between geomorphological units & vegetation types are established with the help of GPS survey and counting of species type. The selected nine environmental transects of Balasore and Kanthi coastal plains i.e. Dagara, Kankadapal, Talsari, Udaipur, Digha, Shankarpur, Tajpur, Mandarmani & Junput have been considered for assessing the role of vegetation in sediment accretion and sediment surface stability. The study shows that the surface stability is achieved with dense vegetation covers and their types in Mangrove Swamp, Salt marsh and sand dunes whereas; the erosional area of geomorphic units has less vegetation cover and insignificant species type. The perfect relationship are found in the shore transect of Balasore-Kanthi coastal interface area (New Digha to Bichitrapur). Finally, it is also observed that the above area is less disturbed by human activities in compare to others.
Особливості мотиваційного та психічного станів жінок другого періоду зрілого віку з надлишковою масою тіла
Authors: Нагорна Анастасія , Андрєєва Олена , Левінська Ксенія
Published: April 2021

Анотація. В статті розглянуто особливості мотивації, якості життя, психічного стану жінок другого періоду зрілого віку з надлишковою масою тіла та ожирінням, визначено провідні мотиви до занять руховою активністю з використанням засобів фітнесу. Мета. Вивчити особливості мотиваційного та психічного станів жінок другого періоду зрілого віку з надлишковою масою тіла як основи побудови самостійних профілактично-оздоровчих занять. Методи. Теоретичний аналіз та узагальнення сучасних даних наукової, методичної та спеціальної літератури, соціологічні, педагогічні, психодіагностичні методи дослідження, методи математичної статистики. Результати. Визначено, що пріоритетними мотивами групи жінок, які взяли участь у дослідженні, є: оздоровлення (71,8 %), естетичні (69,5 %) та особистісні мотиви (49,5 %). Результати досліджень вказують на те, що середньогруповий показник індексу якості життя у жінок другого періоду зрілого віку відповідає середньому рівню і становить 25,7 бала. Аналіз індивідуальних результатів дав змогу виявити 17,1 % жінок з низьким та 20,0 % з високим індексом якості життя. Аналіз індивідуальних результатів, отриманих у ході тестування психічних станів жінок, вказують на низький та середній рівні тривожності, фрустрації, агресивності та ригідності. Отримані результати дали змогу виділити проблемні сфери життєдіяльності у жінок другого періоду зрілого віку з надлишковою масою тіла та встановити чинники, які викликають дискомфорт та незадоволеність якістю життя. Результати дослідження особливостей психічних санів у жінок другого періоду зрілого віку з надлишковою масою тіла вказують на відсутність високого рівня тривожності, фрустрації, агресивності та ригідності. Зазначені особливості покладено в основу розробки програми самостійних занять оздоровчим фітнесом з використанням інформаційних технологій.

Ключові слова: психічний стан, мотивація, надлишкова маса тіла, якість життя, профілактично-оздоровчі заняття, рухова активність, жінки, зрілий вік.
Global Breast Cancer Initiative: an Integrative Approach to Thinking Globally, Acting Locally
Authors: Karbakhsh Mojgan
Published: April 2021

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METACOGNITIVE LISTENING STRATEGY TRAINING: EXPLORING ESL PRE-UNIVERSITY STUDENTS’ METACOGNITIVE AWARENESS AND THEIR PERCEPTION
Authors: Krishnan Saratha , Yaacob Aizan , Veloo Arsaythamby
Published: April 2021

This study examines pre-university students’ metacognitive knowledge and their perception after nine weeks of metacognitive listening strategy training. In total, 31 students were selected from four intact classes in a matriculation college located in the northern region of Malaysia. Students received 9 weeks of Metacognitive Strategy Training (MST) during their listening classes. Focus group interviews were conducted after the strategy training to elicit students’ metacognitive knowledge and their perceptions of MST. Students were divided into five groups for the focus group interviews. Thematic analysis was used to analyse the data. The responses collected from the focus group interviews were reviewed and examined several times to develop themes and subthemes. The findings reveal that students showed a considerable amount of metacognitive knowledge in terms of their role and performance, the process of second language listening, and strategies for listening as second language listeners. Students view MST as helpful and effective to improve their listening skills. Implications for teaching and learning listening in ESL classrooms were discussed.
INTRA KONFLIK DALAM KALANGAN REMAJA MENGANDUNG LUAR NIKAH: PENDEKATAN ART THERAPY
Authors: Musa Mohd Makzan , Chethiyar Saralah Devi Mariamdaran
Published: April 2021

The emergence of unmarried adolescent pregnancy is perceived as a health and social problem resulting in psychosocial consequences. This study discusses the psychological conflicts experienced by adolescents
who become pregnant out of wedlock using the Art Therapy approach which involves methods of scratching, drawing and colouring. Art therapy is a form of creative intervention innovation which allows understanding the burden of psychological conflict borne by the marginal group of adolescents who become pregnant unplanned, unexpected and undesirable. Study data was obtained from the results
of the drawings and followed by unstructured interviews in the effort to understand the psychological conflict experienced by referring to the symbols in the drawings. The findings of the study through the
transcript of unstructured interviews focused on several drawings from the Art Therapy session with reference to the variety of symbols in the study samples’ drawings. The symbols from the study samples’
drawings are grouped by theme based on the psychological conflict of adolescents conceiving and giving birth out of wedlock. This study depicts that the Art Therapy approach can be used to explore and
understand the complicated psychological conflicts experienced by the unmarried pregnant adolescents. Studies prove that the Art Therapy Approach is a form of creative intervention innovation.
The Effect of Transaction Experience Using Digital Wallets on User Satisfaction in Millennial Generation
Authors: Purnama Suryari , Bangun Cicilia Sriliasta , A. Faaroek Safiani
Published: April 2021

The purpose of this study to determine the effect after transaction failure and after failure recovery on the transaction experience, the effect after transaction failure and recovery from transaction failure using digital wallets on user satisfaction, especially in the millennial generation. The population of this study is all digital wallet users in the DKI Jakarta province with a total sample of 94 digital wallet users. The sampling method uses purposive sampling with the criteria of digital wallet users who have made transactions for at least 1 year, this is intended so that these respondents already have transaction experience and are at least 17 years old. Meanwhile, the data processing used Structural Equation Modeling analysis software Lisrel. The results showed: (1) Transaction failure affects the transaction experience of digital wallet users; (2) Transaction failure recovery will affect the transaction experience of digital wallet users; (3) Transaction experience affects the satisfaction of digital wallet users; (4) Transaction failure has no effect on digital wallet user satisfaction; (5) Transaction failure recovery affects the satisfaction of digital wallet users. The novelty of this research is transaction failure has no effect on digital wallet user satisfaction. In addition, the benefits that are felt are far greater than the failures that are experienced, especially when recovery from failure is carried out quickly and precisely.
Viscous Dissipation Effects in A Microchannel Caused by Oscillation of One Surface
Authors: Hao Hor Chee , Ping Tso Chih , Mee Chen Gooi
Published: April 2021

The viscous dissipation effects in a microchannels caused by an oscillatory lower surface is investigated numerically. An asymmetric thermal boundary condition, particularly at upper plate insulated and lower plate with constant surface temperature is solved and analyzed in details graphically. Results reveal that effect of temperature field is strongly dependent on Brinkman number, while the thermal diffusion rate on the heat induced relies on the Prandtl number. The angular frequency has influence on the temperature field gradient.
Internal auditor burnout – an urgent area for research?
Authors: Grzesiak Lena
Published: April 2021

The article explores the problem of professional burnout among internal auditors. The phenomenon of professional burnout has been known for almost five decades, but the knowledge of it is still insufficient. This is a major problem because burnout affects more and more professions and the number of organizations struggling with its impacts is increasing. This paper was inspired by the author’s previous research into internal audit effectiveness. The internal auditors surveyed then reported among others that their profession was more at risk of burnout than many others, and seriously hindered their effectiveness. These findings led the author to conclude that being an internal auditor inherently involved a higher risk of burnout. Hence the aim of this theoretical paper is to assess the urgency of studying professional burnout among internal auditors. In the article question whether or not (and why) more research in this area was necessary, was answered. The article may be an incentive to undertake further research on internal auditor job burnout. In preparing the paper, research methods such as a critical analysis of the literature and content analysis were used. The paper is organised as follows. In Section 1 provides the introduction. Section 2 contains an overview of the risk factors for burnout among internal auditors. In section 3, the directions for future research are proposed based on the presented findings (discussion).
FinTech i FinReg - nowe wyzwania dla systemu regulacji rynku finansowego w Polsce i na świecie
Authors: Gębski Łukasz
Published: April 2021

Skuteczna regulacja rynku finansowego jest kluczowa dla jego innowacyjności i przyszłej pozycji firm FinTech w branży usług finansowych. Przed globalnym kryzysem finansowym innowacje finansowe były postrzegane bardzo pozytywnie, co doprowadziło do deregulacji wielu rynków usług finansowych. W czasie kryzysu nastawienie regulatorów zmieniło się diametralnie. Szybkie zmiany technologiczne pobudziły rozwój firm z branży technologii finansowych (FinTech). FinTech i inni dostawcy usług finansowych otwarcie kwestionują obecny paradygmat regulacyjny. Organy nadzorcze coraz częściej starają się zrównoważyć tradycyjne podejście regulacyjne do stabilności finansowej i ochrony konsumentów z promowaniem wzrostu i innowacji. Wynikające z tego innowacje regulacyjne obejmują technologie, centra innowacji, piaskownice regulacyjne i inne indywidualne rozwiązania. W artykule przeanalizowano nowe podejścia regulacyjne, ustrukturyzowany eksperymentalizm (np. piaskownice regulacyjne) oraz perspektywę nowych ram regulacyjnych.
Comparison of the Sensitivity and Specificity of Tzanck Smear and Immunofluorescence Assay for the Diagnosis of Cutaneous Herpes Simplex Virus and Varicella Zoster Virus Infections in a Real-life Clinical Setting
Authors: Chaiyabutr Chayada , Jantanapornchai Nuttagarn , Apinuntham Chalermkwan , Leeyaphan Charussri , Jiamton Sukhum
Published: April 2021

Objective: This research aim to compare (1) the sensitivities and specificities of Tzanck smears and indirect immunofluorescence assays (IFA) for cutaneous HSV and VZV infections in real-life settings; and (2) the detection rates of the tests for various patient types and lesion morphologies.Materials and Methods: This retrospective study reviewed 440 and 172 samples from patients with clinically suspicious cutaneous HSV and VZV infections, respectively. All patients underwent a Tzanck smear and IFA. The gold standard for the study was agreement of pre- and post-diagnostic coding (determined by a dermatologist) for cutaneous HSV and VZV infections.Results: For HSV infections, the respective sensitivity and specificity of Tzanck smears were 32.8% and 96.6%, whereas those for IFA were 60.7% and 100%. As to VZV infections, the sensitivity and specificity of Tzanck smears were 54.3% and 97.8%, respectively, while the corresponding IFA values were 71.7% and 100%. According to disease characteristics and lesion morphologies, the detection ability of cutaneous HSV by IFA was substantially higher than Tzanck smear especially in immunosuppressed condition.  Tzanck smears and IFA demonstrated no statistical difference for early-onset ( 3 days) VZV infections.Conclusion: Tzanck smears and IFA had higher sensitivities for detecting VZV than HSV infections. IFA testing in suspected cutaneous HSV patients with immunosuppressed conditions should be recommended. Despite the overall sensitivity and specificity of IFA being greater than those for Tzanck smears especially in HSV infections, the latter test is a comparable option for early-onset VZV infections.
Efficacy of Lenalidomide plus Low-Dose Dexamethasone in Thai Patients with Relapsed and/or Refractory Multiple Myeloma
Authors: Kunacheewa Chutima , Siritanaratkul Noppadol
Published: April 2021

Objective: Lenalidomide is an immunomodulatory agent with proven efficacy in the treatment of multiple myeloma. In large global clinical studies, lenalidomide plus dexamethasone has demonstrated significant improvements in the overall response rate and overall survival in patients with relapsed and/or refractory multiple myeloma, compared with a placebo and dexamethasone. This is the first study to report lenalidomide plus low-dose dexamethasone administered in Thai patients. Methods: The aim of this phase II, single-center, single-arm study was to evaluate the efficacy and safety of lenalidomide and low-dose dexamethasone in patients with relapsed and/or refractory multiple myeloma. The primary endpoint was the overall response rate at the fourth treatment cycle. Secondary endpoints included depth of response, time to response, and adverse events. Results: In total, 15 patients with a median age of 61 years old (range 23–74 years old) who had received at least one prior anti-myeloma therapy were enrolled in the study and administered 4-week cycles of lenalidomide 25 mg/day (days 1–21) and dexamethasone 40 mg/week. Patients continued in the study until the occurrence of disease progression or serious adverse events. The overall response rate was 86% and 73.3% at the fourth and from all treatment cycles, respectively (median number of treatment cycles, 10.25), and the median dose for patients aged >60 years old was 15 mg/day. The overall response rate at the fourth cycle in patients who had received prior novel agents (bortezomib and/or thalidomide) was 81.82% compared with 100% in those who had received prior conventional therapy (p = 0.15). The most common adverse events reported were anemia and neutropenia, which were both manageable. Conclusion: Lenalidomide and low-dose dexamethasone was highly effective in Thai patients with relapsed and/or refractory multiple myeloma, with a manageable adverse event profile. These findings suggest that lenalidomide 15 mg/day is a safe and effective dose for Thai patients aged ≥60 years old.
Assessment of Entrepreneurial Behavior Skills among Small Farmers: An Exploratory Study
Authors: Agbolosoo John Atsu , Anaman Richmond
Published: April 2021

Aim - The study assessed the entrepreneurial behavior skills of smallholder potato farmers in Nakuru County, Kenya.
Methodology - A structured questionnaire was used to collect data. Data were collected from a total of 267 respondents using multistage sampling techniques. The principal component analysis was used to check the reliability and construct variability of entrepreneurial behavior skills. An entrepreneurial behavior index was generated to measure the behavior skills of smallholder potato farmers.
Findings - The results show that most smallholder potato farmers had a medium level of risk-taking ability, proactiveness behavior, innovativeness behavior, information-seeking behavior, cosmopoliteness behavior, and decision-making ability. The study concluded that this medium level of entrepreneurial behavior skills made smallholder potato farmers unable to perceive potato farming as a profitable and viable agribusiness venture.
Originality - The study recommends that entrepreneurial training with practical demonstration and effective communication skills should be used as an approach to empower and promote the development of entrepreneurial behavior qualities among smallholder potato farmers.
SISTEM INFORMASI GEOGRAFIS (GIS) PADA RUMAH SAKIT DI KABUPATEN LABUHANBATU BERBASIS WEB
Authors: Ritonga Heri Syahputra , Irmayani Deci , Pane Ramadhani
Published: April 2021

Abstract: Geographical information system (GIS) is a tool with computer and android systems used to map conditions and events that occur on earth. With the current mapping system's capabilities, it can be used by the community. There are still few people who know the hospitals around them, especially with people outside the district. They have to ask the local community to find out the location of the hospital. With a system designed to provide convenience through determining the hospital location points that are available on the system. The data collection process and methods using the waterfall method. Testing this geographic information system with BlackBox. Someone searches Al-Aziz hospital data by selecting the al-Aziz hospital symbol contained in the system. A detailed menu will appear on the symbol so that it provides information about the hospital address, the schedule for opening and closing of the clinic, the name of the doctor, receiving PBJS health patient services, and the doctor's practice schedule at Al-Aziz Hospital. Keywords: Blackbox Testing; Hospital; Location; Waterfall method; Webgis  Abstrak: Sistem Informasi Geografis (SIG) adalah alat komputer dan sistem android yang digunakan untuk memetakan kondisi dan peristiwa yang terjadi di bumi. Dengan kemampuan sistem pemetaan yang ada saat ini dapat dimanfaatkan oleh masyarakat. Masih sedikit masyarakat yang mengetahui rumah sakit di sekitar mereka, terutama yang berada di luar kabupaten. Mereka harus meminta masyarakat sekitar untuk mengetahui lokasi rumah sakit tersebut. Dengan sistem yang dirancang untuk memberikan kemudahan melalui penentuan titik lokasi rumah sakit yang tersedia dalam sistem. Proses dan metode pengumpulan data menggunakan metode waterfall. Uji sistem informasi geografis ini dengan BlackBox. Seseorang mencari data rumah sakit Al-Aziz dengan memilih simbol rumah sakit al-Aziz yang terdapat dalam sistem. Pada simbol ini akan muncul menu detil sehingga memberikan informasi mengenai alamat rumah sakit, jadwal buka tutup klinik, nama dokter, penerimaan pelayanan pasien kesehatan PBJS, dan jadwal praktek dokter di RS Al-Aziz. Kata kunci: Blackbox Testing; Lokasi; Rumah sakit; Waterfall; Webgis
Strategic Social Work Management in Digital Violence Against Women
Authors: Özsungur Fahri
Published: April 2021

This study focuses on determining the strategic social work management principles in digital violence against women. The aim of this study is to determine the strategic factors for the implementation of measures in the determination and management of the causes and effects of violence against women by social work through digital elements. The results of the study showed that the behavioral patterns of digital violence against women were disruptive behavior, bullying, harassment, stalking, intimidating, controlling. It was determined that strategic social work management consisted of analysis and planning, implementation, control, competitive power, feedback, and strategic renewal stages/steps. According to other findings, it was determined that women were exposed to digital violence through computer games, cyberbullying (cyber harassment), cyberwarfare, internet fraud (cybercrime fraud), social media, smartphone applications, e-articles. Recommended applications for the implementation phase of social work are e-gender discrimination measures, women's e-refuge / e-shelter, esports (electronic sports) women.
A importância dos jogos didáticos no processo de ensino aprendizagem: Revisão integrativa
Authors: Almeida Franciane Silva , Oliveira Patricia Batista de , Reis Deyse Almeida dos
Published: April 2021

Jogos lúdicos são metodologias úteis para os processos de ensino-aprendizagem, pois se caracterizam como alternativas que auxiliam a construção de conhecimento pelos alunos. Os jogos e as brincadeiras vêm ganhando espaço e importância em todas as abordagens referentes à infância. A atividade lúdica, que pode se expressar no jogo, no brinquedo ou brincadeira, tem importância fundamental na educação escolar e na formação do homem. Ela permite ao educador perceber traços da personalidade e do comportamento do educando, o que facilita o planejamento de estratégias pedagógicas no ambiente lúdico, promovendo a motivação para uma melhor aprendizagem. O objetivo desta pesquisa foi realizar uma revisão integrativa em plataformas bibliográficas para verificar a eficácia do uso de jogos educacionais no ambiente escolar, buscar também a importância do uso de jogos educativos para o auxílio do profissional docente e identificar o aprendizado dos discentes através de jogos didáticos. Em resultados da revisão foram encontradas 09 publicações no período de 10 anos nos anos de 2010 a 2020. As publicações abordam como a atividade lúdica é essencial no âmbito escolar e como os docentes necessitam de mais recursos e motivação pela escola para melhor aplicação do mesmo.
Utilizing airborne LiDAR data to quantify marsh edge morphology and the role of oyster reefs in mitigating marsh erosion
Authors: Hogan S , Wiberg PL , Reidenbach MA
Published: April 2021

Marsh habitats, experiencing accelerated change, require accurate monitoring techniques. We developed methods to quantify marsh edge morphology using airborne LiDAR data. We then applied these methods within the context of oyster reef restoration within the shallow coastal bays of Virginia, USA, by comparing retreat and morphology quantified at paired reef-lined and control marsh edges at 10 different marsh sites. Retreat metrics were analyzed between 2002 and 2015, utilizing a LiDAR derived edge for the year 2015 from points of maximum slope and aerial imagery pre-2015. Retreat was also compared before and after oyster reef restoration to determine if reefs slow erosion. We found that slope statistics from airborne LiDAR elevation data can accurately capture marsh edge morphology. Retreat rate, measured at edges typically found near the vegetation line, was not significantly different between reef-lined and control marshes and ranged from 0.14 to 0.79 m yr-1. Both retreat rate (ρ = -0.90) and net movement (ρ = -0.88) were strongly correlated to marsh edge elevation. Exposed control marshes had significantly greater mean and maximum slope values compared to reef-lined marshes. The mean edge slope was 11.4° for exposed marshes and 6.0° for reef-lined marshes. We hypothesize that oyster reefs are causing an elongation of the marsh edge by reducing retreat at lower elevations of the marsh edge. Therefore, changes in marsh edge morphology may be a precursor to changes in marsh retreat rates over longer timescales and emphasizes the need for repeated LiDAR measurements to capture processes driving marsh edge dynamics.
Genetic markers validate photo-identification and uniqueness of spot patterns in whale sharks
Authors: Meenakshisundaram A , Thomas L , Kennington WJ , Thums M , Lester E , Meekan M
Published: April 2021

Monitoring the demographics and movement patterns of mobile marine species underpins appropriate management and conservation strategies. Photographic identification of whale sharks Rhincodon typus based on individual variations in spot patterns is a widely used technique for monitoring of populations, but relies on the untested assumptions that these variations in spot patterns are unique to each shark and can be reliably detected using photo-matching software. This study validated the accuracy of photo-identification technique by manually determining the number of photo-identified individuals showing mismatched genetic profiles created for the individuals using 12 microsatellite markers. Results from 154 photographic and genetic identifications of whale sharks were 100% concordant, showing the uniqueness of spot patterns to each shark and high accuracy of the photo-identification technique. Based on these techniques, we observed an annual resighting rate of approximately 10% at Ningaloo Reef, Western Australia, between 2016 and 2018, showing evidence that variations in spot patterns did not change over a time scale of years. Our study shows that the photographic identification technique provides a reliable means to recognise individuals and monitor whale sharks through time.
Bowen, The Bell, and the Late-Modernist Short Story
Authors: D'hoker Elke
Published: April 2021

This essay looks at Elizabeth Bowen's presence in The Bell during the war years. She contributed an essay, a short story, two pieces of memoir, two obituaries, and a few other, smaller pieces to the magazine, but also featured in an interview, several reviews, and O'Faoláin's editorials and critical essays. Yet, as a Protestant, Anglo-Irish woman writer living in England, Bowen was in many ways an odd presence in The Bell, which squarely focused on Irish life and Irish writing. While O'Faoláin's mission to present an inclusive view of Ireland may explain his publication of Bowen's autobiographical essays, her prominence as a fiction writer can better be accounted for through her achievements in the modern short story, the genre O'Faoláin sought to promote as a central Irish literary form in The Bell. Indeed, although Bowen's short stories have been classified as ‘modernist’ and O'Faoláin's as ‘realist’, their aesthetics of the short story are remarkably similar. Still, The Bell’s championing of Bowen's short fiction as a model to follow was undermined by its framing of Bowen as an ‘aristocratic’ writer whose literary snapshots of Irish life had a peculiarly dated and blinkered quality.
MAJOR OBSTETRICAL COMPLICATIONS IN AN ELDERLY PRIMIPAROUS WITH A MULTITUDE OF PRE-EXISTING PATHOLOGIES
Authors: Cristina Vasiliu , Lorena Cojocaru , Patricia Malusanu , Simona Albu Elena , Monica Cîrstoiu
Published: April 2021

The number of primiparous pregnant women, over the age of 35, has increased considerably in the past few years, because of the elevated level of education and also because the multitude of contraceptive methods. Such a patient suits the category of elderly primiparous and it represents a real obstetrical challenge, due to the numerous complications related to pregnancy and the immediate postpartum, as well as those related to the neonatal prognosis. We present a 35 old years patient, second gestation, primiparous, a partially monitored pregnancy, known with essential hypertension grade III ESC / ESA, insufficiently controlled therapeutically, with target organ damage (exudative retinal detachment, hypertensive choroidopathy), morbid obesity and also with the suspicion of a renal tumor. The rate of complications occurring in the case of an elderly primiparous was increased in the present case. Obstetrical complications occurred: preeclampsia, HELLP (hemolysis, elevated liver enzymes, low platelet count) syndrome, placental abnormalities (placenta accreta), intrauterine growth restriction, chronic fetal distress and finally fetal death in utero. The evolution of the patient in postpartum was burdened by the decompensation of the pre-existing pathology. In conclusion, elderly primiparous is susceptible to obstetrical complications during pregnancy and childbirth, such as premature birth, fetal acute distress, which may result in fetal death, placental abruption, placental abnormalities, preeclampsia, eclampsia, therefore following up the pregnancy requires increased attention and is also a real challenge for the obstetrician.
An Extrudable Partially Demineralized Allogeneic Bone Paste Exhibits a Similar Bone Healing Capacity as the “Gold Standard” Bone Graft
Authors: Tournier Pierre , Guicheux Jérôme , Paré Arnaud , Veziers Joëlle , Barbeito Ana , Bardonnet Raphaël , Corre Pierre , Geoffroy Valérie , Weiss Pierre , Gaudin Alexis
Published: April 2021

Autologous bone grafts (BGs) remain the reference grafting technique in various clinical contexts of bone grafting procedures despite their numerous peri- and post-operative limitations. The use of allogeneic bone is a viable option for overcoming these limitations, as it is reliable and it has been widely utilized in various forms for decades. However, the lack of versatility of conventional allogeneic BGs (e.g., blocks, powders) limits their potential for use with irregular or hard-to-reach bone defects. In this context, a ready- and easy-to-use partially demineralized allogeneic BG in a paste form has been developed, with the aim of facilitating such bone grafting procedures. The regenerative properties of this bone paste (BP) was assessed and compared to that of a syngeneic BG in a pre-clinical model of intramembranous bone healing in critical size defects in rat calvaria. The microcomputed tridimensional quantifications and the histological observations at 7 weeks after the implantation revealed that the in vivo bone regeneration of critical-size defects (CSDs) filled with the BP was similar to syngeneic bone grafts (BGs). Thus, this ready-to-use, injectable, and moldable partially demineralized allogeneic BP, displaying equivalent bone healing capacity than the “gold standard,” may be of particular clinical relevance in the context of oral and maxillofacial bone reconstructions.
Activin/Nodal/TGF-β Pathway Inhibitor Accelerates BMP4-Induced Cochlear Gap Junction Formation During in vitro Differentiation of Embryonic Stem Cells
Authors: Fukunaga Ichiro , Oe Yoko , Chen Cheng , Danzaki Keiko , Ohta Sayaka , Koike Akito , Ikeda Katsuhisa , Kamiya Kazusaku
Published: April 2021

Mutations in gap junction beta-2 (GJB2), the gene that encodes connexin 26 (CX26), are the most frequent cause of hereditary deafness worldwide. We recently developed an in vitro model of GJB2-related deafness (induced CX26 gap junction-forming cells; iCX26GJCs) from mouse induced pluripotent stem cells (iPSCs) by using Bone morphogenetic protein 4 (BMP4) signaling-based floating cultures (serum-free culture of embryoid body-like aggregates with quick aggregation cultures; hereafter, SFEBq cultures) and adherent cultures. However, to use these cells as a disease model platform for high-throughput drug screening or regenerative therapy, cell yields must be substantially increased. In addition to BMP4, other factors may also induce CX26 gap junction formation. In the SFEBq cultures, the combination of BMP4 and the Activin/Nodal/TGF-β pathway inhibitor SB431542 (SB) resulted in greater production of isolatable CX26-expressing cell mass (CX26+ vesicles) and higher Gjb2 mRNA levels than BMP4 treatment alone, suggesting that SB may promote BMP4-mediated production of CX26+ vesicles in a dose-dependent manner, thereby increasing the yield of highly purified iCX26GJCs. This is the first study to demonstrate that SB accelerates BMP4-induced iCX26GJC differentiation during stem cell floating culture. By controlling the concentration of SB supplementation in combination with CX26+ vesicle purification, large-scale production of highly purified iCX26GJCs suitable for high-throughput drug screening or regenerative therapy for GJB2-related deafness may be possible.
A Closer Look to the Evolution of Neurons in Humans and Apes Using Stem-Cell-Derived Model Systems
Authors: Schörnig Maria , Taverna Elena
Published: April 2021

The cellular, molecular and functional comparison of neurons from closely related species is crucial in evolutionary neurobiology. The access to living tissue and post-mortem brains of humans and non-human primates is limited and the state of the tissue might not allow recapitulating important species-specific differences. A valid alternative is offered by neurons derived from induced pluripotent stem cells (iPSCs) obtained from humans and non-human apes and primates. We will review herein the contribution of iPSCs-derived neuronal models to the field of evolutionary neurobiology, focusing on species-specific aspects of neuron’s cell biology and timing of maturation. In addition, we will discuss the use of iPSCs for the study of ancient human traits.
Targeted Thrombospondin-1 Expression in Ocular Vascular Development and Neovascularization
Authors: Sorenson Christine M. , Wang Shoujian , Darjatmoko Soesiawati R. , Gurel Zafer , Liu Bo , Sheibani Nader
Published: April 2021

Tight regulation of positive and negative regulators of angiogenesis is essential, particularly in the eye where their dysregulation can lead to vision loss. Thrombospondin-1 (TSP1) is a matricellular protein that negatively regulates angiogenesis and inflammation in the eye. It aids ocular vascular homeostasis such that its loss contributes to increased retinal vascular density and pathologic ocular neovascularization. Our previous studies demonstrated that mice globally lacking TSP1 expression had increased retinal vascular density, decreased hyperoxia-induced retinal vessel loss, and increased choroidal neovascularization. Here we determined the impact to the ocular vasculature of endothelial cell, pericyte, or astrocyte loss of TSP1 expression. Only lack of TSP1 expression in endothelial cells was sufficient to increase choroidal neovascularization with mice lacking expression in pericytes or astrocytes not demonstrating a significant impact. Although the global TSP1 knockout mice demonstrated increased retinal vascular density, individual cell type loss of TSP1 resulted in decreased retinal endothelial cell numbers before and/or after vascular maturation in a cell type specific fashion. Retinas from mice lacking TSP1 expression in endothelial cells, pericytes or astrocytes were not protected from retinal vessel regression in response to hyperoxia as we previously observed in the global knockout. Thus, modulation of TSP1 expression in individual cell types demonstrates a response that is unique to the role TSP1 plays in that cell type of interest, and their coordinated activity is critical for vision.
Two New Antiprotozoal Diterpenes From the Roots of Acacia nilotica
Authors: Anyam John V. , Daikwo Priscilla E. , Ungogo Marzuq A. , Nweze Nwakaego E. , Igoli Ngozichukwuka P. , Gray Alexander I. , De Koning Harry P. , Igoli John O.
Published: April 2021

The powdered roots of the medicinal plant Acacia nilotica were extracted with hexane and ethyl acetate, and the extracts were subjected to column chromatography for the isolation of potentially bioactive compounds and their screening against kinetoplastid pathogens. NMR and HREI mass spectrometric analyses identified two new diterpenes, characterized as 16, 19-dihydroxycassa-12-en-15-one (Sandynone, 1) and (5S, 7R, 8R, 9R, 10S, 13Z, 17S)-7,8:7,17:16,17-triepoxy-7,8-seco-cassa-13-ene (niloticane B, 2). The previously reported (5S,7R,8R,9R,10S) -(-)-7,8-seco-7, 8-oxacassa-13,15-diene-7,17-diol (3), (5S,7R,8R,9R,10S) -(-)-7,8-seco-7, 8-oxacassa-13,15-dien-7-ol-17-al (4), and (5S,7R,8R,9R,10S) -(-)-7,8-seco-7, 8-oxacassa-13,15-dien-7-ol (5) a, mixture of stigmasterol (6a) and sitosterol (6b), and lupeol (7) were also isolated. Several column fractions displayed significant activity against a panel of Trypanosoma and Leishmania spp., and from the most active fraction, compound 4 was isolated with high purity. The compound displayed high activity, particularly against T. brucei, T. evansi, and L. mexicana (0.88–11.7 µM) but only a modest effect against human embryonic kidney cells and no cross-resistance with the commonly used melaminophenyl arsenical and diamidine classes of trypanocides. The effect of compound 4 against L. mexicana promastigotes was irreversible after a 5-h exposure, leading to the sterilization of the culture between 24 and 48 h.
Micro-CT-Based Quantification of Extracted Thrombus Burden Characteristics and Association With Angiographic Outcomes in Patients With ST-Elevation Myocardial Infarction: The QUEST-STEMI Study
Authors: Karagiannidis Efstratios , Papazoglou Andreas S , Sofidis Georgios , Chatzinikolaou Evangelia , Keklikoglou Kleoniki , Panteris Eleftherios , Kartas Anastasios , Stalikas Nikolaos , Zegkos Thomas , Girtovitis Fotios , Moysidis Dimitrios V. , Stefanopoulos Leandros , Koupidis Kleanthis , Hadjimiltiades Stavros , Giannakoulas George , Arvanitidis Christos , Michaelson James S. , Karvounis Haralambos , Sianos Georgios
Published: April 2021

Background: Angiographic detection of thrombus in STEMI is associated with adverse outcomes. However, routine thrombus aspiration failed to demonstrate the anticipated benefit. Hence, management of high coronary thrombus burden remains challenging. We sought to assess for the first time extracted thrombotic material characteristics utilizing micro-computed tomography (micro-CT).

Methods: One hundred thirteen STEMI patients undergoing thrombus aspiration were enrolled. Micro-CT was undertaken to quantify retrieved thrombus volume, surface, and density. Correlation of these indices with angiographic and electrocardiographic outcomes was performed.

Results: Mean aspirated thrombus volume, surface, and density (±standard deviation) were 15.71 ± 20.10 mm3, 302.89 ± 692.54 mm2, and 3139.04 ± 901.88 Hounsfield units, respectively. Aspirated volume and surface were significantly higher (p < 0.001) in patients with higher angiographic thrombus burden. After multivariable analysis, independent predictors for thrombus volume were reference vessel diameter (RVD) (p = 0.011), right coronary artery (RCA) (p = 0.039), and smoking (p = 0.027), whereas RVD (p = 0.018) and RCA (p = 0.019) were predictive for thrombus surface. Thrombus volume and surface were independently associated with distal embolization (p = 0.007 and p = 0.028, respectively), no-reflow phenomenon (p = 0.002 and p = 0.006, respectively), and angiographically evident residual thrombus (p = 0.007 and p = 0.002, respectively). Higher thrombus density was correlated with worse pre-procedural TIMI flow (p < 0.001). Patients with higher aspirated volume and surface developed less ST resolution (p = 0.042 and p = 0.023, respectively).

Conclusions: Angiographic outcomes linked with worse prognosis were more frequent among patients with larger extracted thrombus. Despite retrieving larger thrombus load in these patients, current thrombectomy devices fail to deal with thrombotic material adequately. Further studies of novel thrombus aspiration technologies are warranted to improve patient outcomes.

Clinical Trial Registration: QUEST-STEMI trial ClinicalTrials.gov number: NCT03429608 Date of registration: February 12, 2018. The study was prospectively registered.