bluuebunny/crossref_metadata_2025 huggingface dataset:
Sensitivity of SARS-CoV-2 Variants to Neutralization by Convalescent Sera and a VH3-30 Monoclonal Antibody
Authors: Yue Shuai , Li Zhirong , Lin Yao , Yang Yang , Yuan Mengqi , Pan Zhiwei , Hu Li , Gao Leiqiong , Zhou Jing , Tang Jianfang , Wang Yifei , Tian Qin , Hao Yaxing , Wang Juan , Huang Qizhao , Xu Lifan , Zhu Bo , Liu Pinghuang , Deng Kai , Wang Li , Ye Lilin , Chen Xiangyu
Published: September 2021

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic of novel coronavirus disease (COVID-19). Though vaccines and neutralizing monoclonal antibodies (mAbs) have been developed to fight COVID-19 in the past year, one major concern is the emergence of SARS-CoV-2 variants of concern (VOCs). Indeed, SARS-CoV-2 VOCs such as B.1.1.7 (UK), B.1.351 (South Africa), P.1 (Brazil), and B.1.617.1 (India) now dominate the pandemic. Herein, we found that binding activity and neutralizing capacity of sera collected from convalescent patients in early 2020 for SARS-CoV-2 VOCs, but not non-VOC variants, were severely blunted. Furthermore, we observed evasion of SARS-CoV-2 VOCs from a VH3-30 mAb 32D4, which was proved to exhibit highly potential neutralization against wild-type (WT) SARS-CoV-2. Thus, these results indicated that SARS-CoV-2 VOCs might be able to spread in convalescent patients and even harbor resistance to medical countermeasures. New interventions against these SARS-CoV-2 VOCs are urgently needed.
Air-Sea Gas Fluxes and Remineralization From a Novel Combination of pH and O2 Sensors on a Glider
Authors: Possenti Luca , Humphreys Matthew P. , Bakker Dorothee C. E. , Cobas-García Marcos , Fernand Liam , Lee Gareth A. , Pallottino Francesco , Loucaides Socratis , Mowlem Matt Charles , Kaiser Jan
Published: September 2021

Accurate, low-power sensors are needed to characterize biogeochemical variability on underwater glider missions. However, the needs for high accuracy and low power consumption can be difficult to achieve together. To overcome this difficulty, we integrated a novel sensor combination into a Seaglider, comprising a spectrophotometric lab-on-a-chip (LoC) pH sensor and a potentiometric pH sensor, in addition to the standard oxygen (O2) optode. The stable, but less frequent (every 10 min) LoC data were used to calibrate the high-resolution (1 s) potentiometric sensor measurements. The glider was deployed for a 10-day pilot mission in August 2019. This represented the first such deployment of either type of pH sensor on a glider. The LoC pH had a mean offset of +0.005±0.008 with respect to pH calculated from total dissolved inorganic carbon content, c(DIC), and total alkalinity, AT, in co-located water samples. The potentiometric sensor required a thermal-lag correction to resolve the pH variations in the steep thermocline between surface and bottom mixed layers, in addition to scale calibration. Using the glider pH data and a regional parameterization of AT as a function of salinity, we derived the dissolved CO2 content and glider c(DIC). Glider surface CO2 and O2 contents were used to derive air-sea fluxes, Φ(CO2) and Φ(O2). Φ(CO2) was mostly directed into the ocean with a median of −0.4 mmol m–2 d–1. In contrast, Φ(O2) was always out of the ocean with a median of +40 mmol m–2 d–1. Bottom water apparent oxygen utilization (AOU) was (35±1) μmol kg–1, whereas apparent carbon production (ACP) was (11±1) μmol kg–1, with mostly insignificant differences along the deployment transect. This deployment shows the potential of using pH sensors on autonomous observing platforms such as Seagliders to quantify the interactions between biogeochemical processes and the marine carbonate system at high spatiotemporal resolution.
Effects of Epibiotic Diatoms on the Productivity of the Calanoid Copepod Acartia tonsa (Dana) in Intensive Aquaculture Systems
Authors: Pan Yen-Ju , Wang Wei-Lung , Hwang Jiang-Shiou , Souissi Sami
Published: September 2021

We evaluated here the effects of the epibiotic diatom Tabularia sp. on the productivity of the calanoid copepod Acartia tonsa (Dana) for assessing their risk on copepod intensive aquaculture industry for the provision of live feed. In the first experiment, uninfested and intensively infested females were cultivated individually for the assessment of egg production. Intensively infested females appeared to have a significantly lower egg production (5.0–9.0 eggs/female/d) than uninfested females (22.0–26.0 eggs/female/d) during 5 consecutive days. In the second experiment, effects of culture densities on diatom epibiosis were investigated in 9 L cultures at three different densities (200, 400, and 600 ind. L–1). Another culture at higher volume (250 L) and lowest density (200 ind. L–1) was also carried out to test the effect of culture volume on diatom epibiosis. The infestation rate (%), infestation intensity (ratio of surface diatom coverage levels, classified as levels 0–3) and daily egg harvest rate (number of harvested eggs per day per liter) were evaluated among the four culture populations. The copepods had higher infestation rate (53.69–60.14%) and intensity rate (high ratios at level 2 and 3) when the densities were increased from 200 ind./L to 400 and 600 ind./L. Although egg harvest increased with increasing culture density, it seemed that the diatom-infested A. tonsa population reach a saturated egg production when the density was higher than 400 ind./L. Nevertheless, the differences of culture volumes (250 and 9 L) appeared to be not to have any effect when the copepods were cultivated at the same density (200 ind./L). This study reveals for the first time that the epibiosis of the diatom Tabularia sp. reduces the individual egg production, and egg harvest rate in high-density culture of the copepod A. tonsa. Our findings implicate that diatom epibiosis should be avoid in copepod intensive culture systems.
A Clustering Approach for Predicting Dune Morphodynamic Response to Storms Using Typological Coastal Profiles: A Case Study at the Dutch Coast
Authors: Athanasiou Panagiotis , van Dongeren Ap , Giardino Alessio , Vousdoukas Michalis , Antolinez Jose A. A. , Ranasinghe Roshanka
Published: September 2021

Dune erosion driven by extreme marine storms can damage local infrastructure or ecosystems and affect the long-term flood safety of the hinterland. These storms typically affect long stretches (∼100 km) of sandy coastlines with variable topo-bathymetries. The large spatial scale makes it computationally challenging for process-based morphological models to be used for predicting dune erosion in early warning systems or probabilistic assessments. To alleviate this, we take a first step to enable efficient estimation of dune erosion using the Dutch coast as a case study, due to the availability of a large topo-bathymetric dataset. Using clustering techniques, we reduce 1,430 elevation profiles in this dataset to a set of typological coastal profiles (TCPs), that can be employed to represent dune erosion dynamics along the whole coast. To do so, we use the topo-bathymetric profiles and historic offshore wave and water level conditions, along with simulations of dune erosion for a number of representative storms to characterize each profile. First, we identify the most important drivers of dune erosion variability at the Dutch coast, which are identified as the pre-storm beach geometry, nearshore slope, tidal level and profile orientation. Then using clustering methods, we produce various sets of TCPs, and we test how well they represent dune morphodynamics by cross-validation on the basis of a benchmark set of dune erosion simulations. We find good prediction skill (0.83) with 100 TCPs, representing a 93% input and associated computational costs reduction. These TCPs can be used in a probabilistic model forced with a range of offshore storm conditions, enabling national scale coastal risk assessments. Additionally, the presented techniques could be used in a global context, utilizing elevation data from diverse sandy coastlines to obtain a first order prediction of dune erosion around the world.
The Role of Peritoneal Dialysis in the Treatment of Acute Kidney Injury in Patients With Acute-on-Chronic Liver Failure: A Prospective Brazilian Study
Authors: Ponce Daniela , Zamoner Welder , Dias Dayana Bitencourt , Pires da Rocha Erica , Kojima Christiane , Balbi André Luís
Published: September 2021

This study aimed to explore the role of peritoneal dialysis (PD) in acute-on-chronic liver disease (ACLD) in relation to metabolic and fluid control and outcome. Fifty-three patients were treated by PD (prescribed Kt/V = 0.40/session), with a flexible catheter, tidal modality, using a cycler and lactate as a buffer. The mean age was 64.8 ± 13.4 years, model of end stage liver disease (MELD) was 31 ± 6, 58.5% were in the intensive care unit, 58.5% needed intravenous inotropic agents including terlipressin, 69.5% were on mechanical ventilation, alcoholic liver disease was the main cause of cirrhosis and the main dialysis indications were uremia and hypervolemia. Blood urea and creatinine levels stabilized after four sessions at around 50 and 2.5 mg/dL, respectively. Negative fluid balance (FB) and ultrafiltration (UF) increased progressively and stabilized around 3.0 L and −2.7 L/day, respectively. Weekly-delivered Kt/V was 2.7 ± 0.37, and 71.7% of patients died. Five factors met the criteria for inclusion in the multivariable analysis. Logistic regression identified as risk factors associated with Acute Kidney Injury (AKI) in ACLD patients: MELD (OR = 1.14, CI 95% = 1.09–2.16, p = 0.001), nephrotoxic AKI (OR = 0.79, CI 95% = 0.61–0.93, p = 0.02), mechanical ventilation (OR = 1.49, CI 95% = 1.14–2.97, p < 0.001), and positive fluid balance (FB) after two PD sessions (OR = 1.08, CI 95% = 1.03–1.91, p = 0.007). These factors were significantly associated with death. In conclusion, our study suggests that careful prescription may contribute to providing adequate treatment for most Acute-on-Chronic Liver Failure (ACLF) patients without contraindications for PD use, allowing adequate metabolic and fluid control, with no increase in the number of infectious or mechanical complications. MELD, mechanical complications and FB were factors associated with mortality, while nephrotoxic AKI was a protective factor. Further studies are needed to better investigate the role of PD in ACLF patients with AKI.
Case Report: Successful Treatment of Five Critically Ill Coronavirus Disease 2019 Patients Using Combination Therapy With Etoposide and Corticosteroids
Authors: Aoyagi Tetsuji , Sato Yukio , Baba Hiroaki , Shiga Takuya , Seike Issei , Niitsuma Sugaya Ikumi , Takei Kentarou , Iwasaki Yudai , Oshima Kengo , Kanamori Hajime , Yoshida Makiko , Saito Koji , Tokuda Koichi , Kaku Mitsuo
Published: September 2021

Acute respiratory distress syndrome (ARDS) is the leading cause of mortality in hospitalized patients with coronavirus disease 2019 (COVID-19) because of limited effective therapies. During infection, the accumulation and activation of macrophages and monocytes in the lungs induce inflammatory mediators and contribute to tissue injury, leading to ARDS. However, therapeutic strategies that directly target activated macrophage and monocytes have not been reported. Combination treatment with etoposide (a cytotoxic agent) and a corticosteroid has been widely used for treating hemophagocytic lymphohistiocytosis characterized by the systemic activation of macrophages with overwhelming inflammation. Herein, we present five cases of COVID-19-associated ARDS treated with etoposide and corticosteroids. Three of the five patients were over 65 years of age and had various underlying diseases, including multiple myeloma. Four patients required invasive mechanical ventilation (MV), and one patient refused to be placed on MV due to underlying diseases. All patients were pre-treated with antiviral and/or other anti-inflammatory agents, but their condition deteriorated and hyperinflammation was noted. All five patients responded well to treatment and had an immediate response, as reflected by improvement in their respiratory condition and inflammatory marker levels and rapid resolution of fever after etoposide administration; however, some patients required a second dose of etoposide and longer course of steroids. All patients recovered, and there were no severe adverse events related to the drugs. Following successful treatment in these five patients, we plan to conduct a clinical trial to evaluate the efficacy and safety of combination therapy with etoposide and corticosteroid for treating COVID-19 patients in Japan.
Prompt Reduction in CRP, IL-6, IFN-γ, IP-10, and MCP-1 and a Relatively Low Basal Ratio of Ferritin/CRP Is Possibly Associated With the Efficacy of Tocilizumab Monotherapy in Severely to Critically Ill Patients With COVID-19
Authors: Hashimoto Shoji , Yoshizaki Kazuyuki , Uno Kazuko , Kitajima Heita , Arai Tsuyoshi , Tamura Yoshitaka , Morishita Hiroshi , Matsuoka Hiroto , Han Yuki , Minamoto Seijiro , Hirashima Tomonori , Yamada Tomoki , Kashiwa Yozo , Kameda Makoto , Yamaguchi Seiji , Tsuchihashi Yasunari , Iwahashi Mitsuhiro , Nakayama Emi , Shioda Tatsuo , Nagai Takayuki , Tanaka Toshio
Published: September 2021

Background and Aim: Tocilizumab, a humanized anti-IL-6 receptor antibody, has been used to treat severely to critically ill patients with COVID-19. A living systematic review with meta-analysis of recent RCTs indicates that the combination therapy of corticosteroids and tocilizumab produce better outcomes, while previous observational studies suggest that tocilizumab monotherapy is beneficial for substantial numbers of patients. However, what patients could respond to tocilizumab monotherapy remained unknown.

Methods: In this retrospective study we evaluated the effects of tocilizumab monotherapy on the clinical characteristics, serum biomediator levels, viral elimination, and specific IgG antibody induction in 13 severely to critically ill patients and compared with those of dexamethasone monotherapy and dexamethasone plus tocilizumab.

Results: A single tocilizumab administration led to a rapid improvement in clinical characteristics, inflammatory findings, and oxygen supply in 7 of 11 patients with severe COVID-19, and could recover from mechanical ventilation management (MVM) in 2 patients with critically ill COVID-19. Four patients exhibited rapidly worsening even after tocilizumab administration and required MVM and additional methylprednisolone treatment. Tocilizumab did not delay viral elimination or inhibit IgG production specific for the virus, whereas dexamethasone inhibited IgG induction. A multiplex cytokine array system revealed a significant increase in the serum expression of 54 out of 80 biomediators in patients with COVID-19 compared with that in healthy controls. Compared with those who promptly recovered in response to tocilizumab, patients requiring MVM showed a significantly higher ratio of basal level of ferritin/CRP and a persistent increase in the levels of CRP and specific cytokines and chemokines including IL-6, IFN-γ, IP-10, and MCP-1. The basal high ratio of ferritin/CRP was also associated with clinical deterioration even in patients treated with dexamethasone and tocilizumab.

Conclusion: Tocilizumab as monotherapy has substantial beneficial effects in some patients with severe COVID-19, who showed a relatively low level of the ratio of ferritin/CRP and prompt reduction in CRP, IL-6, IFN-γ, IP-10, and MCP-1. The high ratio of ferritin/CRP is associated with rapid worsening of pneumonia. Further evaluation is warranted to clarify whether tocilizumab monotherapy or its combination with corticosteroid is preferred for severely to critically ill patients with COVID-19.
Androgen Receptor Targeted Therapy + Radiotherapy in Metastatic Castration Resistant Prostate Cancer
Authors: Massaro Maria , Facondo Giuseppe , Vullo Gianluca , Aschelter Anna Maria , Rossi Alessandro , De Sanctis Vitaliana , Marchetti Paolo , Osti Mattia Falchetto , Valeriani Maurizio
Published: September 2021

Objectives

To investigate whether radiotherapy as metastasis-directed therapy (MDT) on oligo-progressive sites in metastatic castration-resistant prostate cancer (mCRPC) patients during treatment with androgen receptor-targeted therapy (ARTT) may lead to control resistant lesions, prolonging ARTT. We analysed progression free survival, overall survival and prognostic parameters that can identify patients that best suit to this approach.

Patients and Methods

Retrospective analysis of a total of 67 lesions in 42 mCRPC patients treated with ablative or palliative RT to oligoprogressive lesions during ARTT. Twenty-eight patients (67%) underwent ARTT with Abiraterone acetate and 14 patients (33%) underwent ARTT with Enzalutamide. Median time between the start of ADT and ARTT beginning was 50.14 months (range 3.37-219 months). We treated 58 lesions (87%) with 3D conformal radiotherapy (3DCRT) and nine lesions (13%) with stereotactic body radiotherapy (SBRT). The Kaplan Meier method was used to assess the median overall survival (OS) and the progression-free survival (PFS).

Results

Median follow-up was 28 months (range 3-82 months). Median OS was 32.5 months (95% CI 25.77-39.16), 1 and 2-year OS were 71.6% and 64.1%, respectively. Median PFS was 19,8 months (95% CI 11.34–28.31), 1 and 2-year PFS were 67.2% and 47.4%, respectively. Median OS for patients that underwent radiotherapy before 6 months from the start of ARTT was 23.4 months (95% CI 2.04-44.89) and 45.5 months (95% CI 31.19-59.8) for patients that underwent radiotherapy after 6 months (p = 0.009).

Conclusion

Local ablative radiation therapy directed to progressive metastasis is a non-invasive, well tolerated treatment with efficacy on prolonging clinical benefit of systemic therapies with ARTT. Patients who underwent RT >6 months from the start of ARTT presented a statistically better OS and PFS compared with patients who underwent radiotherapy <6 months from the start of ARTT.
Functional and Oncological Outcomes Following Robot-Assisted and Laparoscopic Radical Prostatectomy for Localized Prostate Cancer With a Large Prostate Volume: A Retrospective Analysis With Minimum 2-Year Follow-Ups
Authors: Deng Wen , Liu Xiaoqiang , Liu Weipeng , Zhang Cheng , Zhou Xiaochen , Chen Luyao , Guo Ju , Wang Gongxian , Fu Bin
Published: September 2021

Objective

We aimed to analyze the perioperative, functional, and oncologic outcomes following robot-assisted radical prostatectomy (RARP) and laparoscopic radical prostatectomy (LRP) for patients with localized prostate cancer (PCa) characterized by a large prostate volume (PV; ≥50 ml) over a minimum of 2 years follow-up.

Materials and Methods

Patients undergoing RARP and LRP for localized PCa with a large PV were included in the final analysis. The perioperative, functional, and oncologic outcomes were analyzed between the two groups.

Results

All operations were successfully completed without open conversion in both groups. The mean operative time and estimated blood loss in the RARP group were significantly decreased compared to those in the LRP group (139.4 vs. 159.0 min, p = 0.001, and 124.2 vs. 157.3 ml, p = 0.003, respectively). Patients in the RARP arm had significantly lower proportions of grade II or lower and of higher than grade II postoperative complications compared with those in the LRP group (7.9% vs. 17.1%, p = 0.033, and 1.6% vs. 6.7%, p = 0.047, respectively). No significant differences in terms of the rates of pT3 disease, positive surgical margin, and positive lymph node were noted between the two groups. Moreover, no significant difference in the median specimen Gleason score was observed between the RARP and LRP groups (6 vs. 7, p = 0.984). RARP vs. LRP resulted in higher proportions of urinary continence upon catheter removal (48.4% vs. 33.3%, p = 0.021) and at 3 (65.1% vs. 50.5%, p = 0.025) and 24 (90.5% vs. 81.0%, p = 0.037) months post-operation. The median erectile function scores at 6 and 24 months post-operation in the RARP arm were also significantly higher than those in the LRP arm (15 vs. 15, p = 0.042, and 15 vs. 13, p = 0.026, respectively). Kaplan–Meier analyses indicated that the biochemical recurrence-free survival and accumulative proportion of continence were statistically comparable between the two groups (p = 0.315 and p = 0.020, respectively).

Conclusions

For surgically managing localized PCa with a large prostate (≥50 ml), RARP had a tendency toward a lower risk of postoperative complications and better functional preservation without cancer control being compromised when compared to LRP.
Birth Weight and Subsequent Risk of Total Leukemia and Acute Leukemia: A Systematic Review and Meta-Analysis
Authors: Che Hailuo , Long Dunmei , Sun Qian , Wang Lina , Li Yunbin
Published: September 2021

Objective:Birth weight, an important indicator of fetal nutrition and degree of development, may affect the risk of subsequent leukemia. At present, little is known about the effect of birth weight on acute myeloid leukemia (AML) and whether there is a dose-dependent relationship of birth weight with acute lymphoid leukemia (ALL) and AML. To address these questions, the present work aimed to systematically investigate the relationship between birth weight and the risk of subsequent leukemia based on the current epidemiological studies

Methods:Relevant studies were systematically retrieved from electronic databases PubMed, Embase, and Cochrane Library, from inception to May 15th, 2021. Finally, 28 studies (including 21 case-control studies and 7 cohort studies) were included for the final meta-analysis. Results in cohort studies were performed by risk ratios (RRs), while those in case-control studies by odds ratios (ORs), and all results were assessed by adopting the random-effect model. Besides, a dose-dependent analysis was conducted based on the cohort studies.

Results:Compared with the population with normal birth weight (NBW), the population with high birth weight (HBW) might have an increased risk of leukemia (OR 1.33, 95%CI 1.20–1.49;I20%). Meanwhile, low birth weight (LBW) was associated with a decreased risk of ALL, as evidenced from the pooled analysis of case-control studies (OR 0.83, 95% CI 0.75–0.92;I223.3%). However, relative to NBW population, the HBW population might have an increased risk of ALL (OR 1.28, 95% CI 1.20–1.35;I27%). There was no obvious evidence supporting the relationship between LBW and the risk of AML from the pooled analysis of case-control studies (OR, 1.11 95% CI 0.87–1.42;I231.7%).

Conclusions:Overall, in children and young adults, HBW population may be associated with the risks of subsequent leukemia and AML relative to NBW population, but the supporting dose-dependent evidence is lacking. In addition, compared with NBW population, there is stronger evidence supporting a significantly increased risk of subsequent ALL in HBW population, and a decreased risk in LBW population in a dose-dependent manner. More prospective studies with large samples are warranted in the future to validate and complement these findings.
Case Report: Novel Copy Number Variant 16p11.2 Duplication Associated With Prune Belly Syndrome
Authors: Talluri Sriharsha , Goedde Michael A. , Rosenberg Eran , Canalichio Katie L. , Peppas Dennis , White Jeffrey T.
Published: September 2021

Prune belly syndrome (PBS) is a rare congenital disease that predominantly occurs in males and is identified by its classic triad of abdominal wall musculature deficiencies, cryptorchidism, and urinary tract abnormalities. However, numerous anomalies involving the kidneys, heart, lungs, and muscles have also been reported. A multitude of chromosomal abnormalities have been implicated in its pathogenesis. PBS can occur in association with trisomy 18 and 21. Gene duplications and deletions have also been reported; however, a definite cause of PBS is still unknown. We report the first PBS patient with a copy number variant in 16p11.2.
Preliminary Experiences With Robot-Assisted Choledochal Cyst Excision Using the Da Vinci Surgical System in Children Below the Age of One
Authors: Xie Xiaolong , Wu Yang , Li Kewei , Ai Chengbo , Wang Qi , Wang Chuan , Chen Jing , Xiang Bo
Published: September 2021

The purpose of this study is to introduce our preliminary experiences with using the da Vinci surgical system to treat choledochal cysts in children under 1 year old and discuss the application of this robot-assisted surgery. We retrospectively analyzed all available clinical data of children below the age of 1 who underwent surgery for choledochal cysts using the da Vinci robotic surgical system between January 2015 and December 2020. Data collection mainly included demographic information, imaging data, perioperative details, and postoperative outcomes. Ten patients were included in this study. The average patient age was 8.5 months, and the average weight was 9.11 kg. Half of these patients suffered from abdominal pain, while 30% exhibited vomiting and 10% jaundice. Eight of them were type Ia, and two were Ic. The average operation time among the patients was 219.5 min. None of the 10 patients had to receive a blood transfusion or conversion. The average time of the patients' subsequent fluid diet was 3.28 days, and the solid diet was 3.76 days. Meanwhile, the average length of hospital stay was 7.6 days. All 10 patients recovered and were eventually discharged. We believe that the da Vinci surgical system is a safe and feasible form of treatment for choledochal cysts in children <1 year old.
The Role of the Thalamus in Declarative and Procedural Linguistic Memory Processes
Authors: Crosson Bruce
Published: September 2021

Typically, thalamic aphasias appear to be primarily lexical-semantic disorders representing difficulty using stored declarative memories for semantic information to access lexical word forms. Yet, there also is reason to believe that the thalamus might play a role in linguistic procedural memory. For more than two decades, we have known that basal ganglia dysfunction is associated with difficulties in procedural learning, and specific thalamic nuclei are the final waypoint back to the cortex in cortico-basal ganglia-cortical loops. Recent analyses of the role of the thalamus in lexical-semantic processes and of the role of the basal ganglia in linguistic processes suggest that thalamic participation is not simply a matter of declarative vs. procedural memory, but a matter of how the thalamus participates in lexical-semantic processes and in linguistic procedural memory, as well as the interaction of these processes. One role for the thalamus in accessing lexical forms for semantic concepts relates to the stabilization of a very complex semantic-lexical interface with thousands of representations on both sides of the interface. Further, the possibility is discussed that the thalamus, through its participation in basal ganglia loops, participates in two linguistic procedural memory processes: syntactic/grammatical procedures and procedures for finding words to represent semantic concepts, with the latter interacting intricately with declarative memories. These concepts are discussed in detail along with complexities that can be addressed by future research.
Adolescent Life Satisfaction Explained by Social Support, Emotion Regulation, and Resilience
Authors: Azpiazu Izaguirre Lorea , Fernández Arantzazu Rodríguez , Palacios Eider Goñi
Published: September 2021

Adolescence is a stage characterized by many biological and psychosocial changes, all of which may result in a decrease in subjective well-being. It is therefore necessary to identify those factors that contribute to increased life satisfaction, in order to promote positive development among young people. The aim of this study is to examine the dynamics of a set of variables that contribute to life satisfaction. A total of 1,188 adolescents (aged between 12 and 16 years) completed the Perceived Social Support from Family and Friends and Perception of the School Environment Questionnaires, the Trait Meta Mood Scale (TMMS), Connor-Davidson Resilience Scale-10 (CD-RISC), and Satisfaction with Life Scale (SWLS) for social support, emotion regulation, resilience, and life satisfaction. By applying structural equation modeling (SEM), the results reveal a direct prediction of family support, emotion regulation, and resilience on life satisfaction. Support from friends and emotion regulation was also found to explain resilience, and support from family and teachers was found to predict emotion regulation. In conclusion, emotion regulation and social support were found to indirectly affect life satisfaction among adolescents through resilience. The theoretical and practical implications of these results are discussed.
Syrian Refugee Youth Resettled in Norway: Mechanisms of Resilience Influencing Health-Related Quality of Life and Mental Distress
Authors: Dangmann Cecilie , Solberg Øivind , Myhrene Steffenak Anne Kjersti , Høye Sevald , Andersen Per Normann
Published: September 2021

Background: The importance of resilience factors in the positive adaptation of refugee youth is widely recognised. However, their actual mechanism of impact remains under-researched. The aim of this study was therefore to explore protective and promotive resilience mechanisms to inform future interventions and policy. Promotive resilience is seen as a direct main effect and protective resilience as a moderating effect.

Methods: This was a cross-sectional study of Syrian youth recently resettled in Norway, aged 13–24 years. Regression and moderation analyses were used to explore different interactions, including moderated mediation using the PROCESS macro for SPSS.

Result: A direct main effect of promotive resilience was found for health-related quality of life (HRQoL) and general mental distress, but not for post-traumatic stress disorder (PTSD). No moderating effects of protective resilience were found. Post-migration stressors mediated the effects of potentially traumatic events (PTE) from war and flight, and this indirect effect was present at all levels of resilience.

Conclusion: Despite high risk exposure and mental distress, resilience was also high. Broad resilience interventions targeting multiple factors would likely benefit the group, but these should include both individual assets and building supportive environments. Additionally, reducing current stress and providing treatment for those in need could enable recovery and increase the efficacy of resilience factors already present.
Comparison of Different Staging Systems Applied to a Cohort of Patients With Oral Tongue and Floor of the Mouth Cancer
Authors: Bresciani Lorenzo , Giannini Lorenzo , Paderno Alberto , Incandela Fabiola , Fontanella Walter , Mattavelli Davide , Piazza Cesare
Published: September 2021

Purpose: The present work compares the effects produced by the application of the 7th edition of the tumor node metastasis (TNM) staging system (TNM7), 8th Edition (TNM8) with its two subsequent revisions, and pN-N+ classification on a cohort of patients with oral tongue and floor of the mouth cancer.

Methods: A monocentric cohort of 148 patients was retrospectively analyzed. Patients were staged according to the TNM7, TNM8 and revisions, and pN-N+ classification. Stage migration was assessed and overall survival (OS) analyzed with the Kaplan–Meier method. The pT, pN, and stage stratification was evaluated with univariate and multivariate Cox regression and comparing adjacent categories with the log-rank method.

Results: pT3-T4a categories showed significant differences in comparison to pT1-T2 for each staging metric employed in both uni- and multivariate analysis. When comparing adjacent pT categories, OS was significantly different only between pT2 and pT3 categories of the TNM8. Disproportionate patient distribution among pN categories was observed in the TNM8, and stratification was scarce. Conversely, in the pN-N+ classification the difference between pN2 and pN3a categories was significant. Only stage IVa reached statistical significance in TNM7, whereas stage III and above were significant in TNM8 and revisions in both uni- and multivariate analysis. However, no significant difference was noted comparing adjacent stages.

Conclusion: The TNM8 pT classification differentiated low- from high-risk diseases. Nonetheless, it failed to separate pT1 from pT2 and pT3 from pT4a categories. Conversely, although TNM8 nodal staging was inaccurate, the number of metastatic lymph nodes was more valuable.
Mechanical Properties of Novel Reinforced Spray in Place Pipe Material With Potential Fully Structural Performance Application
Authors: Azimi M. Amin , Matthews John C. , Bagherpour Asal , Alsadi Alhossin , Kraft John J. , Alam Shaurav , Campos Urso
Published: September 2021

Many utility owners in the United States are actively searching for new and innovative rehabilitation techniques to help them regain control and repair large tracts of their networks; moreover, traditional open cut replacement is too costly and slow to keep up with demand. Many trenchless techniques such as Spray-In-Place Pipe (SIPP) lining have been adopted by municipalities to renew their aging pipeline systems. The further development of SIPP linings could benefit the water community in the United States and around the world. SIPP lining could play a key role in controlling the future expected burden caused by the aging distribution networks. This study aimed to investigate the mechanical properties of a newly proposed reinforced spray in place pipe (SIPP) lining material that boasts structural characteristics. This material is a spray applied two component polyurethane high build coating. Three forms of sample were investigated during these tests: (1) No Fiber-Baseline (2) Rigid-with one layer of carbon fiber (3) Rigid—with two layers of carbon fiber. Three types of tests are performed: tensile, flexural, and hardness tests. This study included both experimental and Finite Element Analysis (FEA). Target mechanical properties of proposed material can be improved by using carbon fiber (CF) as reinforcement and based on the results of this study, the SIPP lining material with two layers of CF reinforcement has the highest value of 8,067 psi and 16,534 psi during the tensile and flexural test, respectively.
The Clever Strategies That Fishes Use to Survive in San Francisco’s Dynamic Estuary
Authors: Fichman Rachel A. , Khen Adi , Willmes Malte , Kuntz Jonathon , Scott Alexander R. , Hobbs James A. , Lewis Levi S.
Published: September 2021

Estuaries are places where fresh water from rivers mixes with salty water from the ocean. Why does this matter? This mixing creates dynamic, ever-changing conditions that fishes must navigate in order to survive. Environmental conditions can change yearly, seasonally, daily, and even hourly. Fishes use many different strategies to adjust to this wild variation. Some are picky and only use certain habitats. Others use many different kinds of habitats and move between them at different times of the year. Adults and juveniles might even live away from each other in different parts of the estuary. In this article, we describe some of the clever strategies that fishes use to survive in estuaries. We also consider how scientists uncover these clever strategies and what each of us can do to help protect the fishes of the San Francisco Estuary and elsewhere.
Updated Evaluation of Laparoscopic vs. Open Appendicectomy During Pregnancy: A Systematic Review and Meta-Analysis
Authors: Zhang Jia , Wang Miye , Xin Zechang , Li Ping , Feng Qingbo
Published: September 2021

To explore the updated evaluation about the obstetrical and perioperative outcomes of laparoscopic appendicectomy (LA) for pregnancy appendicitis compared with open appendicectomy (OA). Two reviewers independently searched the PubMed, the Cochrane Central Register of Controlled Trials, EMBASE, and Web of Science databases to screen eligible studies up to December 2020. Only clinical researches, no < 10 cases for LA and OA group were included. Twenty retrospective studies with 7,248 pregnant women, evaluating LA and OA in surgical and obstetrical outcomes, were included. The weighted mean difference (WMD) with 95% CI and odds ratio (OR) was used to compare continuous and dichotomous variables. It seems LA was connected with significantly shorter hospital time and lower wound infection [mean difference (MD), −0.57 days; 95% CI, −0.96 to −0.18;p= 0.004 and OR, 0.34; 95% CI, 0.18 to 0.62;p= 0.0005, respectively]. The incidence of fetal loss after LA was higher than OA (OR,1.93; 95% CI, 1.39–2.69;p< 0.0001). It was almost similar in the rate of preterm delivery (OR, 0.80; 95% CI, 0.48 to 1.34;p= 0.40) and other perioperative and obstetrical complications (p> 0.05). Our results indicated that the occurrence of fetal loss after LA should not be ignored. Caution, skillful operation, and thoroughly informed consent about the advantages and disadvantages of laparoscopy are necessary.

Systematic Review Registration:https://www.crd.york.ac.uk/PROSPERO/#recordDetails, identifier: CRD42021233150.
Evaluating the Feasibility and Efficacy of a Dual-Modality Nanoparticle Contrast Agent (Nanotrast-CF800) for Image-Guided Sentinel Lymph Node Mapping in the Oral Cavity of Healthy Dogs
Authors: Wan Jennifer , Oblak Michelle L. , Ram Ann S. , McKenna Charly , Singh Ameet , Nykamp Stephanie
Published: September 2021

A combination of pre and intraoperative sentinel lymph node (SLN) mapping techniques have been suggested to optimize SLN detection. A novel liposomal nanoparticle, Nanotrast-CF800 (CF800), utilizes computed tomography lymphography (CTL) and near infrared fluorescence imaging (NIRF) for image-guided surgery and SLN mapping. This novel tracer agent has not been evaluated in companion animals. The objective of this study was to evaluate the feasibility and efficacy of CF800 for SLN mapping in the oral cavity of healthy dogs and to report any local adverse effects. Six healthy adult purpose-bred research dogs randomly received either 1 mL (group 1) or 2 mL (group 2) of CF800 injected into the submucosa at the level of the right canine maxillary tooth. CTL and percutaneous NIRF were performed at 1, 3, and 10 min, then 1, 2, 4, 7, and 10 days post-injection (p.i). Overall, both CTL and NIRF identified SLNs in all dogs. The overall peak mean contrast enhancement of the SLNs was 73.98 HU (range 63.45–86.27 HU) at 2 days p.i. Peak fluorescence of the SLN occurred at 1 day p.i. The agent was retained within the SLN for at least 7 days for CTL and 4 days for percutaneous NIRF. No adverse effects were observed. Local administration of CF800 was simple and feasible for the detection of SLNs using CTL+NIRF in the head and neck of healthy dogs and was not associated with significant local adverse events.
Contagious Ovine Digital Dermatitis: A Novel Bacterial Etiology and Lesion Pathogenesis
Authors: Staton Gareth J. , Angell Joseph W. , Grove-White Dai , Clegg Simon R. , Carter Stuart D. , Evans Nicholas J. , Duncan Jennifer S.
Published: September 2021

Contagious ovine digital dermatitis (CODD) is a severe and common infectious foot disease of sheep and a significant animal welfare issue for the sheep industry in the UK and some European countries. The etiology and pathogenesis of the disease are incompletely understood. In this longitudinal, experimental study, CODD was induced in 18 sheep, and for the first time, the clinical lesion development and associated microbiological changes in CODD affected feet are described over time, resulting in a completely new understanding of the etiopathogenesis of CODD. The majority of CODD lesions (83.9%) arose from pre-existing interdigital dermatitis (ID) and/or footrot (FR) lesions. All stages of foot disease were associated with high levels of poly-bacterial colonization with five pathogens, which were detected by quantitative PCR (qPCR): Treponema medium, Treponema phagedenis, Treponema pedis, Dichelobacter nodosus, and Fusobacterium necrophorum. Temporal colonization patterns showed a trend for early colonization by T. phagedenis, followed by F. necrophorum and D. nodosus, T. medium, and then T. pedis, D. nodosus was present at significantly higher predicted mean log10 genome copy numbers in FR lesions compared to both ID and CODD, while Treponema species were significantly higher in CODD and FR lesions compared to ID lesions (p < 0.001). Treatment of CODD-affected sheep with two doses of 10 mg/kg long acting amoxicillin resulted in a 91.7% clinical cure rate by 3 weeks post-treatment; however, a bacteriological cure was not established for all CODD-affected feet. The study found that in an infected flock, healthy feet, healed CODD feet, and treated CODD feet can be colonized by some or all of the five pathogens associated with CODD and therefore could be a source of continued infection in flocks. The study is an experimental study, and the findings require validation in field CODD cases. However, it does provide a new understanding of the etiopathogenesis of CODD and further supportive evidence for the importance of current advice on the control of CODD; namely, ensuring optimum flock control of footrot and prompt isolation and effective treatment of clinical cases.
Utility of Computed Tomographic Angiography for Pulmonary Hypertension Assessment in a Cohort of West Highland White Terriers With or Without Canine Idiopathic Pulmonary Fibrosis
Authors: Soliveres Eugénie , Mc Entee Kathleen , Couvreur Thierry , Fastrès Aline , Roels Elodie , Merveille Anne-Christine , Tutunaru Alexandru-Cosmin , Clercx Cécile , Bolen Géraldine
Published: September 2021

West Highland white terriers (WHWTs) affected with canine idiopathic pulmonary fibrosis (CIPF) are at risk of developing precapillary pulmonary hypertension (PH). In humans, thoracic computed tomography angiography (CTA) is commonly used to diagnose and monitor patients with lower airway diseases. In such patients, CTA helps to identify comorbidities, such as PH, that could negatively impact prognosis. Diameter of the pulmonary trunk (PT), pulmonary trunk-to-aorta ratio (PT/Ao), and right ventricle-to-left ventricle ratio (RV/LV) are CTA parameters commonly used to assess the presence of PH. Pulmonary vein-to-right pulmonary artery ratio (PV/PA) is a new echocardiographic parameter that can be used in dogs to diagnose PH. The primary aim of this study was to evaluate the use of various CTA parameters to diagnose PH. An additional aim was to evaluate the correlation of RV/LV measurements between different CTA planes. CTA and echocardiography were prospectively performed on a total of 47 WHWTs; 22 affected with CIPF and 25 presumed healthy control dogs. Dogs were considered to have PH if pulmonary vein-to-right pulmonary artery ratio (PV/PA) measured on 2D-mode echocardiography was less than to 0.7. WHWTs affected with CIPF had higher PT/Ao compared with control patients. In WHWTs affected with CIPF, PT size was larger in dogs with PH (15.4 mm) compared with dogs without PH (13 mm, p = 0.003). A cutoff value of 13.8 mm predicted PH in WHWTs affected with CIPF with a sensitivity of 90% and a specificity of 87% (AUC = 0.93). High correlations were observed between the different CTA planes of RV/LV. Results suggest that diameter of the PT measured by CTA can be used to diagnose PH in WHWTs with CIPF.
Effect of BLV Infection on the Immune Function of Polymorphonuclear Neutrophil in Dairy Cows
Authors: Lv Guanxin , Wang Hai , Wang Jianfa , Lian Shuai , Wu Rui
Published: September 2021

Enzootic bovine leukemia is a late-onset, neoplastic infection caused by the bovine leukemia virus (BLV). BLV infection hinders the function of the immune system and induces other diseases, which negatively affects the performance and health of the infected cows. As the first line of defense against invading foreign pathogenic microorganisms, polymorphonuclear neutrophil (PMN) plays a vital role in the immune system of dairy cows. However, research on the effect of BLV infection on the immune function of PMN in dairy cows is scarce. Therefore, this experiment aimed to elucidate the effects and effect mechanisms of BLV infection on the immune function of PMN in dairy cows with different BLV provirus loads by detecting the chemotaxis, migration, adhesion, phagocytosis, respiratory burst function, and the formation of NETs. The experimental results showed that BLV infection had no significant effect on the phagocytosis of PMN but inhibited their migration and respiratory burst function, and the effects were closely related to the BLV provirus load. Under high BLV provirus load, PMN produced large amounts of NETs, chemokine CXCL7, adhesion molecule CD18, and pro-inflammatory factors IL-8 and TNF-α, triggering inflammatory responses, and tissue damage. The results of this study will help reveal the reason why BLV infection causes the high incidence of mammary gland inflammation in dairy cows.
Section Optimization Design of a Flexible Cable-Bar Tensile Structure Based on Robustness
Authors: Chen Lianmeng , Zeng Yihong , Gao Weifeng , Liu Yijie , Zhou Yiyi
Published: September 2021

As the current literature lacks effective nonlinear robustness evaluation method and optimal design theory of the structural robustness for flexible cable-bar tensile structure, this paper aimed to conduct further studies. Based on the H∞ theory, a fundamental robustness analysis method and a detailed calculation way through the combination of induction of L2 performance criterion and random theory for nonlinear structural robustness quantitative evaluation method were proposed. Following this, a real Geiger cable dome structure was studied as its research object, and the influences of structural robustness of simultaneous changes of all elements section and changes of every kind of element section were analysed, respectively. Finally, the genetic algorithm was applied through MATLAB and ANSYS software to achieve optimal section layout, with the goal of minimizing structural quality on the condition that the structural robustness indicator keep less than that of the initial structure. The result revealed that the increase of the section of elements can effectively enhance structural robustness and the section changes of various elements showed different sensitivities to the influence of structural robustness. Meanwhile, structural quality can be effectively reduced by optimizing measures such as increasing the section of elements with significant effect on structural robustness and reducing the section of elements with minor effects on structural robustness, while the structural robustness indicator keeps less than that of the initial structure. The optimization reveals that quality was reduced by 42.5% in this paper.