bluuebunny/crossref_metadata_2025 huggingface dataset:
Software Konsultasi Seleksi Karir Siswa menggunakan Metode Certainy Factor
Authors: Irwan Irwan , Gustientiedina Gustientiedina , Hajjah Alyauma , Desnelita Yenny , Susanti Wilda
Published: February 2021

Model software konsultasi seleksi karir sebagai alternatif  pengambilan keputusan dimana  konsultasi meliputi pemahaman karir, perencanaan karir, alternatif pilihan karir yang berupa alat penelusuran minat dan bakat siswa terhadap keputusan karir siswa menggunakan metode certainty factor berbasis sistem pakar. Penelitian ini menghasilkan model software konseling dalam memperoleh informasi penting tentang pengembangan karir siswa dan membantu memfasilitasi perkembangan individu siswa melalui bentuk layanan agar mampu merencanakan karirnya berdasarkan jurusan, minat, bakat, pengetahuan, kepribadian, kompetensi dan faktor-faktor lain yang mendukung kemajuan dirinya dalam menentukan pilihan dan keputusan yang sesuai dengan dunia kerja pilihan siswa.Sistem diuji menggunakanwhite box dan black boxdengan menunjukan hasil dimana sistem dapat digunakan sesuai kebutuhan.

 

Abstract

Career selection consulting software model as an alternative decision making where consultation that includes career understanding,career understanding, career planning, alternative career choices in the form of a tool to trace students' interests and talents towards student career decisions using expert system based certainty factor method. This research produces a counseling software model in obtaining important information about student career development and helps facilitate individual students development  through a form of service in order to be able to plan his career based on majors, interests, talents, knowledge, personality, competencies and other factors that support his progress in determining choices and decisions that are appropriate to the world of work choice. The system is tested using white boxes and black boxes by showing the results where the system can be used as needed.
ANALISIS PENDAPATAN DAN TINGKAT KESEJAHTERAAN RUMAH TANGGA PETANI SAYURAN DALAM PROGRAM TANGGUNG JAWAB SOSIAL PERUSAHAAN (Studi Kasus di Kelompok Tani Harapan Jaya Binaan PT Kaltim Prima Coal)
Authors: Lathifaturrahmah Lathifaturrahmah , Tridakusumah Ahmad Choibar
Published: February 2021

Companies as national economic development actors must be able to contribute to improve the welfare of the community and the surrounding environment, this concern is proved by the Corporate Social Responsibility (CSR) program, including PT. Kaltim Prima Coal, which has implemented Social Responsibility for Agibusiness Development for farmers around the mine since 1999. One of the groups that becomes the beneficiary is Harapan Jaya Farmer Group. The purpose of this study is (1) Describe the application of Social Responsibility/CSR of PT. KPC in Harapan Jaya farmer group, (2) Find out the income and expenditure structure of vegetable farmer households who are members of Harapan Jaya farmer group, (3) Find out the welfare level of vegetable farmer households. This study uses a qualitative design with a case study method. The selection of study sites was carried out deliberately (purposive) with the consideration that the area is the ring I area closest to the mining area. The welfare level of household was measured using the poverty line of the Sayogyo (1996), World Bank, and BPS. The welfare level of farmers households based on Sayogyo's criteria (1996), World Bank and based on BPS poverty line criteria continued is criteria shows that 78.95% of farmer households are classified as not poor, 57.89 % of households are classified as poor and 52.63% of farmer households are classified as poor.
FAKTOR-FAKTOR YANG MEMPENGARUHI PENURUNAN POPULASI TERNAK KAMBING PERANAKAN ETAWA (Studi Kasus pada Kelompok Ternak Sukapala Kecamatan Kawalu Kota Tasikmalaya)
Authors: Novianty Ane , Andrie Benizar M.
Published: February 2021

Etawa breeders are faced with a situation with a limited amount of capital, production factor and knowledge in an effort to achieve their goal. This condition causes the decline of PE goat’s population year by year even though the product produced has a strategic opportunity in the market. The purpose of this study is to determine the factors that influence the decline of PE goat’s population in Sukapala herd. The study is carried out in Sukapala herd, Kawalu district, Tasikmalaya city  which consists of 9 people by using a case study method. The factors that influence the decline of PE goat’s population based on multiple linear regression test are capital (X1), treatment and maintenance system (X2), seed’s quality (X3), and livestock breeds quality (X4). Based on the results of partially and simultaneously test, the four independent variables influence significantly in the decline of PE goat’s population and have a negative effect.
PERSEPSI MASYARAKAT DAN DAMPAK SOSIAL EKONOMI TERHADAP LAHAN BEKAS TAMBANG TIMAH SEBAGAI KAMPOENG REKLAMASI AIR JANGKANG DI DESA RIDING PANJANG KABUPATEN BANGKA
Authors: Asparita Vinda , Agustina Fournita , Pranoto Yudi Sapta
Published: February 2021

Mining activities not only cause impacts that occur at the mine site but also the surrounding environment, so that reclamation is needed. This study aims to describe the community's perception of the existence of Kampoeng Air Jangkang Reclamation and to describe the socio-economic impact of the community on the existence of the Jangkang Water Reclamation Kampoeng. This research was conducted from September 2019 to September 2020 in Riding Panjang Village, Merawang District, Bangka Regency. The method used in this research is a survey method. Meanwhile, the sampling method used in this study was purposive sampling. The number of samples taken in this study were 93 samples from the people of Riding Panjang Village. The analytical method used is a qualitative descriptive method with a Likert scale. The results showed that the community's perception of the ex-mining land as Kampoeng Reclamation was strongly agreed with an index number of 82.4 percent covering the pre-reclamation stages, 81.4 percent covering the implementation of Kampoeng Reclamation development and an index figure of 88.8 percent covering the success of Kampoeng development. Reclamation. The social impacts that occur include positive impacts both on environmental conditions and the safety of the communities around the reclamation area. The resulting economic impact is a positive impact on people's income and employment.
KAJIAN EKONOMI USAHATANI HORTIKULTURA PADA KELOMPOK WANITA TANI PERKOTAAN DI BOGOR
Authors: Suwarnata Anak Agung Eka , Sonani Nia , Rosiana Agista
Published: February 2021

The role of farmer groups in urban areas is very much needed in the development of horticultural commodities because it is estimated that the demand for horticultural commodities will increase as the population increases. This is certainly a good business opportunity for urban farmers, especially urban female farmer groups (KWT). The purpose of this research is to examine economically horticultural farming in urban KWT in Bogor in terms of income aspects and the efficiency of using farm costs. The research location was at 52 KWT Bogor City from June-November 2020. Collecting data using questionnaires, survey techniques, interview observation, and documentation study. The study used farm income analysis, while the efficiency analysis was measured by the R/C ratio. The results of the economic study showed that the average cash income of horticultural farming at KWT in Bogor per year per area of 487 m2 was IDR 3,514,080.75 or equivalent to IDR 7,203,865.55 / year / 10 are, while the average total income worth IDR 1,190,686.16 per 487 m2 or equivalent to IDR 2,440,906.62 / year / 10 are. The R / C value of the ratio of cash costs is 4.24, and the R / C ratio of total costs, the value is 1.37, which means that horticultural farming in KWT in Bogor is efficient to run because the targeted input compared to the actual input is greater or equal to one.
AKSELERASI INOVASI TEKNOLOGI SPESIFIK LOKASI STRATEGI PENINGKATAN PRODUKSI DI MASA PANDEMI COVID-19
Authors: Elizabeth Roosganda
Published: February 2021

Food is the most basic and foremost human need, whose adequacy and quality determine the level of intelligence and quality of human resources. One of the important and strategic aspects of agriculture can be seen from the national/regional contribution (GDP/GDPR). Agriculture remains the main priority for development policies in Indonesia, especially during the Covid 19 pandemic, which is still endemic today. This paper, which is a review of various information data and related literature, aims to present various levels of technology application, levels of technical efficiency in various agro-ecosystems, which are presented in a qualitative deductive manner. The occurrence of the global financial crisis (for the short term) and climate change (for the long term) as well as national disasters (for example the Covid 19 pandemic) resulted in a weakening of the economy, especially its growth, increased unemployment, and decreased purchasing power. This condition causes access to food to decline, so that food security is predicted to be more vulnerable. It is necessary to provide technology and information according to needs, adequate government and operational policy tools, the functioning of various supporting institutions (research, extension, marketing) that are more focused and take sides in accelerating the achievement of location-specific technological innovations. This is the right strategy for fulfilling national food. Evaluation of agricultural development policies from the aspect of food institutions is needed as a strategy and measures for the accuracy of policy formulation in supporting future increases in national rice production.
A relação da microbiota intestinal na síndrome do intestino irritável
Authors: De Oliveira Mayra Loures , Leite Nestor Augusto Avelino , Manoel Agnes Zanotto , Flor Bárbara Gonçalves , Silva Bruna de Sousa , Madeiro Dandara Lucena Medeiros , Alfredo Julia Perito , Baratela Maria Cecília , Romanisio Nicole Hana , Manoel Poliana Zanotto
Published: February 2021

Este artigo de revisão buscou discutir acerca da relação da microbiota intestinal na síndrome do intestino irritável, a qual é uma das doenças do trato gastrointestinal mais comum no mundo e leva a alterações dos hábitos intestinais gerando dor e desconforto abdominal. Sua fisiopatologia ainda não é bem estabelecida, entretanto, estudos recentes demonstram que a alteração da microbiota intestinal pode ser a desencadeadora principal dessa doença. Este artigo tem como objetivo estudar a maneira que este desequilíbrio da microbiota intestinal possa influenciar no desenvolvimento ou na exacerbação da síndrome do intestino irritável. A disbiose, que é a interrupção da relação simbiótica no intestino, gera a facilitação para a invasão e crescimento de espécies patogênicas no trato gastrointestinal levando ao desequilíbrio da homeostase do sistema imunológico e da barreira mucosa. Dessa forma, essa alteração das bactérias colonizadoras do intestino podem levar a uma alteração na barreira intestinal e na expressão de proteínas antimicrobianas levando a síndrome do intestino irritável. Diante disso, alguns probióticos vem sendo testados para o tratamento de síndrome do intestino irritável, visto que eles têm como objetivo a recomposição da microbiota e o reestabelecimento do equilíbrio intestinal.
Empowering of reproductive health of farm animals through genome editing technology
Authors: Dua Seema , Bajwa Kamlesh Kumari , Prashar Atul , Bansal Sonu , Beniwal Madhuri , Kumar Pradeep , Punetha Meeti , Selokar Naresh Lalaji , Yadav P. S. , Kumar Dharmendra
Published: February 2021

To cater the exponential growth of human population, need to improve food production and quality through modern biotechnology with limited recourses in a way that has minimal impact on the environment. The selective breeding and genomic selection have attended the momentum gain in livestock productivity. Recent advancement in genome-editing technologies offers exciting prospects for the production of healthy and prolific livestock. Genome editing involves altering genetic material by manipulation, addition, or removal of certain deoxyribonucleic acid (DNA) sequences at a specific locus in a way that does not occur naturally. The major genome editors are zinc finger nucleases, transcription-activator-like endonucleases, and clustered regularly interspaced short palindromic repeats associated protein nine systems which are proficient of cutting the nuclear DNA precisely at a predetermined position. This review provides an update on the use of genome editing systems to modify the genes related to reproduction of farm animal vis-à-vis human, update knowledge on the underlying mechanism and discusses new opportunities to produce genetically modified farm animals.
Biomarkers in ovarian cancer and saliva: An update
Authors: Chandra Kumari Binita , Yadav Savita
Published: February 2021

Asymptomatic nature at the initial stage and heterogeneity makes ovarian cancer a “silent killer” which is being considered as most lethal gynecological cancer by acquiring the fifth leading cause of cancer-related deaths in women. Detection of ovarian cancer frequently requires painful invasive procedures such as multiple biopsies and blood tests which results in an undue stress and discomforts in patient. Recently, saliva is being opted as an alternative source for biomarker discovery due to procedure being non-invasive collection method cost-effectiveness, ease of sample collection, and handling. Saliva, a multiconstituent oral fluid secretion from the major and minor salivary glands enriched with proteins, DNAs, RNAs, and metabolites, behaves as a “mirror of the body.” Salivary diagnostics has become booming field with the development of new and advanced technologies accompanying with proteomics, RNA sequencing, liquid biopsy, and point-of-care (POC) diagnostics. Reliable and reproducible biomarkers identified through advanced salivaomics techniques such as proteomics, transcriptomics, genomics, and metabolomics for oral and systemic diseases including cancers can serve as a diagnostic and monitoring tool. Scientific communities are engaged in developing new technologies for the identification and validation of an extensive range of salivary biomarkers that will provide clinical and scientific credibility for saliva. This review provides a comprehensive update about the significant salivary biomarkers identified by the omics method that can be used for the early detection of ovarian cancer. In coming years, salivaomics may become a very important tool for early detection of ovarian cancer and salivary biomarkers may translate into improvement of treatment outcome and increased survival rate.
Gynecological outcomes of uterine niche after cesarean section: A descriptive study
Authors: Abdelfattah E. A. , Dayem T. M. Abd-El , Galal H. M. , Taylon S. S.
Published: February 2021

Objectives:

Our aim was to study the prevalence of isthmocele in cases with previous cesarean section scar presented to El Shatby maternity university hospital and its association with gynecological complications.

Material and Methods:

After approval of the ethics committee of Alexandria faculty of medicine, a sample of 300 patients delivered by cesarean section since 6 months or more presented by one or more of the following symptoms: Abnormal uterine bleeding (AUB) dysmenorrhea, secondary infertility, and lower abdominal pain were selected from the gynecology clinic of El Shatby maternity university hospital. All patients were subjected to history taking, clinical, and gynecological examination. Post-menstrual 2D transvaginal ultrasonography was done. Where the niche or isthmocele was seen as triangular or dome- shaped echo-free space. Data were collected and entered to the computer using Statistical Package for the Social Science program for statistical analysis.

Results:

Niche was found in 44 cases. Most of them were symptomatic. Duration from the last (CS) was statistically significant. Symptoms were related to number of previous cesarean sections.

Conclusion:

The incidence of post-cesarean section niche in El Shatby Maternity hospital was 14.67%. Most common symptoms were AUB and dyspareunia.
Sperm a cell in distress: Yoga to the rescue
Authors: Dhawan Vidhu , Kumar Rajeev , Malhotra Neena , Dadhwal Vatsla , Borthakur Dibakar , Dada Rima
Published: February 2021

Sperm, one of the complex cells of biological inheritance, are not only considered as mere vectors of transmission of paternal genome at the time of fertilization but also to events post-fertilization. The contribution of sperm molecular factors as a critical determinant of optimal embryonic development and pregnancy outcomes has been brought to surface. Spermatozoa with derangements in redox status, nuclear and mitochondrial genomic integrity, and dysregulated gene expression may affect the fertility status of the male and may result in impaired embryonic development and increase risk of genetic and epigenetic diseases in offspring. The integration of yoga-based lifestyle (YBL) as a part of the modern lifestyle has been found to be beneficial in the management of the derangements in the male reproductive functions in the distressing issue of infertility and early pregnancy loss (EPL) patients. As infertility and EPL are issues with a strong psychosomatic component, yoga a mind body intervention may be a useful adjunctive therapy in the management of these cases and may not only improve the sperm quality but also positively impact the reproductive potential, embryogenesis, and the health of the progeny. This short communication intends to highlight the importance of paternal factors as a causal factor for infertility, EPL, and also the promising role of integration of YBL in the management of such disorders.
Evaluation of the clinical and economic effectiveness of newborn screening programs for inborn errors of metabolism
Authors: Печатникова Н.Л. , Захарова Е.Ю. , Ижевская В.Л.
Published: February 2021

В статье обобщены данные по клинико-экономической оценке программ массового неонатального скрининга на наследственные болезни обмена веществ (НБО). Обсуждаются разные подходы к их клинико-экономической оценке эффективности, влияние внедрения программ расширенного скрининга методом тандемной масс-спектрометрии на бюджет.

The article summarizes the data on the clinical and economic evaluation of newborn screening programs for inborn errors of metabolism (IEM). Various approaches to the clinical and economic evaluation of the effectiveness of newborn screening programs, the impact of expanding screening programs by MS/MS on the budget are discussed.
The search of SNP associated with moderate-to-severe and severe psoriasis apremilast therapy outcome
Authors: Вербенко Д.А. , Карамова А.Э. , Артамонова О.Г. , Дерябин Д.Г. , Кубанов А.А.
Published: February 2021

Псориаз - хроническое воспалительное заболевание кожи мультифакториальной природы с доминирующим значением в развитии генетических факторов, характеризующееся ускоренной пролиферацией эпидермоцитов и нарушением их дифференцировки, дисбалансом между провоспалительными и противовоспалительными цитокинами. Накапливающиеся данные ассоциативных исследований предрасположенности к заболеванию обнаружили значительное количество однонуклеотидных полиморфизмов, расположенных в участках генома, связанных с функционированием иммунной системы. Систематический обзор вариабельности SNP в исследованиях предрасположенности к развитию псориаза позволил сформировать панель 84 SNP маркеров, потенциально значимых для возникновения псориаза и в развития заболевания. Исследование SNP проведено сочетанием высокопроизводительного генотипирования на микрочипах Illumina и компьютерного импьютинга. В подгруппах пациентов с различной эффективностью терапии селективным ингибитором клеточного фосфорилирования «апремиласт» обнаружены различия для трех SNP rs12307915, rs2227473 и rs744166, что открывает возможности прогнозирования эффективности терапии этим препаратом.

Psoriasis is a chronic inflammation skin disease with complex genetic architecture that affects approximately 2% of the world population. The condition is characterized by abnormal keratinocyte hyperproliferation and differentiation related to a dysregulated immune system governed by a pro-inflammatory cytokine network. The accumulating data of psoriasis associative studies reveals significant number of SNPs located in genome sites related with immune system. A review of sources available allowed the selection of potentially significant for psoriasis occurrence and development SNP set, includes 84 markers. SNP determination has been carried out by a combination of high-performance genotyping on Illumina microchips and computer imputing. The differences in three SNPs rs12307915, rs2227473 and rs744166 were found in patient groups with different apremilast therapy outcome, paving the way to the development of predictive psoriasis therapy approaches.
Reflection and biosociality: autonomy, responsibility and informed consent for biobank
Authors: Белялетдинов Р.Р.
Published: February 2021

Иосоциализация общества представляет собой комплекс явлений, среди которых в философском и этическом аспекте выделяются технонаучный подход к знанию, повышение роли автономии и проблема рефлексии. Для биобанков - это комплекс проблем, который реализуется как сложность разработки информированного согласия. Проблема подлинного информированного согласия является крайне важной для успеха биобанкинга. Если ученые рассматривают информированное согласие как формальность, то сами доноры биобанков, осознавая, что их биоматериалы, возможно, используются некорректно в одном из множества проектов, которые осуществляются на базе биобанка, могут не испытывать чувство справедливости и согласия с условиями, которые им предлагаются в рамках расширенного информированного согласия. Сохранение идеи информированности является важным условием формирования позитивного образа биобанкинга.

The biosocialization of society is a complex of phenomenon, among which, in a philosophical and ethical aspect, the techno-scientific approach to knowledge, the increasing role of autonomy and the problem of reflection stand out. For biobanks, this complex of problems is realized as the complexity of developing informed consent. The issue of genuine informed consent is critical to the success of biobanking. If scientists consider informed consent as a formality, then biobank donors themselves, realizing that their biomaterials may not be used correctly in one of the many projects that are implemented on the basis of biobanks, may not feel a sense of fairness and agreement with the conditions that are offered to them within broad informed consent. Maintaining the idea of awareness is an important condition for the formation of a positive biobanking image.
Legal support for the creation and use of genetic technologies in medicine
Authors: Лапаева В.В.
Published: February 2021

Правовая политика России в сфере создания и применения генетических технологий в медицине не обеспечивает в должной мере баланс между системой прав человека, гарантирующих защиту его достоинства и охрану здоровья, и свободой научного творчества. Несогласованность этих прав человека проявляется в том, что при отсутствии запретов на исследования с применением технологий наследуемого редактирования генома человека законодательство лишает патентоспособности любые технологии по модификации генетической целостности клеток зародышевой линии человека. Последовательный правовой подход предполагает введение ограничений на возможность геномного редактирования зародышевой линии и запрета на патентование способов такого редактирования, не выходящего за рамки заданных ограничений. При разработке такого похода целесообразно учесть опыт Великобритании.

The Russian legal policy in the field of the medicine genetic technologies creation and application does not provide a proper balance between the system of human rights that guarantee the protection of its dignity and health, and the freedom of scientific creativity. The non-coordination of these human rights is manifested in the fact that in the absence of prohibitions on research using technologies of human genome inherited editing, the law deprives patentability of any technology for modifying the genetic integrity of human germline cells. A consistent legal approach involves the restrictions on the possibility of the germ line genomic editing and the prohibition of patenting the methods of such editing, which does not go beyond the given restrictions. It is advisable to take into account the experience of Great Britain.
Ethics and laws of using genomic samples in clinical trials of medicines
Authors: Ревазова Ю.А.
Published: February 2021

Приведены и обсуждены документы, регламентирующие использование геномных проб в клинических испытаниях лекарств.

Documents regulating the use of genomic samples in clinical trials of medicines are presented and discussed.
Trust as a social basis for the development of human embryo genome editing technologies
Authors: Тищенко П.Д.
Published: February 2021

Доверие является необходимым условием эффективного функционирования сообществ в различных областях человеческой деятельности, но в особенности в медицине. Развитие технологий редактирования генома эмбрионов человека, как утверждают разработчики, обеспечит возможность создания новых форм медицинской помощи людям, страдающим тяжелыми генетически детерминированными заболеваниями. Вместе с тем, существуют серьёзные риски внесения в геном случайных неблагоприятных изменений, которые будут передаваться потомкам. Предполагается, что начало клинических испытаний этих технологий будет возможно после доказательства их клинической значимости, безопасности и моральной приемлемости. Другим необходимым условием должно выступить отношение доверия к экспериментаторам, включающее транспарентность исследований, общественную и правовую подотчётность, ответственность перед пациентами и их семьями за возможные неблагоприятные последствия.

Trust is a prerequisite for the effective functioning of communities in various fields of human activity, but especially in medicine. The development of human embryo genome editing technologies, according to the developers, will provide the opportunity to create new forms of medical care for people suffering from severe genetically determined diseases. At the same time, there are serious risks of introducing into the genome random adverse changes that will be transmitted to descendants. It is assumed that the start of clinical trials of these technologies will be possible after proving their clinical relevance, safety and moral acceptability. Another necessary condition should be the attitude of trust towards experimenters, including transparency of research, public and legal accountability, responsibility to patients and their families for possible adverse consequences.
The effect of new water-soluble derivatives of C60 fullerene having identical substituents and one substitution CL→ME on gene expression and the activity of transcription factors NRF2 and NFkB
Authors: Савинова Е.А. , Ершова Е.С. , Родионов И.В. , Вейко Н.Н. , Краевая О.А. , Трошин П.А. , Костюк С.В.
Published: February 2021

С применением новых наносоединений, в частности, производных фуллерена С60, возрастает вероятность контакта с ними человека. Показано, что водорастворимые производные фуллерена могут влиять на транскрипционную активность генов в эмбриональных фибробластах легких человека (ФЛЭЧ). Активация транскрипционного фактора NFkB (nuclear factor kappa-light-chain-enhancer of activated B cells) индуцирует процессы, направленные на выживание клеток, а также развитие провоспалительного ответа. Экспрессия генов, обеспечивающих защиту от стресса на клеточном уровне, запускается фактором транскрипции, NRF2 (nuclear factor [erythroid-derived 2]-like factor 2). Взаимодействие между NFkB и NRF2 в основном носит характер антагонизма. Поиск новых соединений, избирательно активирующих транскрипционный фактор NFkB и NRF2, остаётся актуальной задачей современной медицины.

With the use of new nanocompounds, in particular, derivatives of fullerene C60, the likelihood of human contact with them increases. It has been shown that water-soluble derivatives of fullerene can affect the transcriptional activity of genes in human embryonic fibroblasts of the lungs (PLEC). Activation of the transcription factor NFkB (nuclear factor kappa-light-chain-enhancer of activated B cells) induces processes aimed at cell survival, as well as the development of a pro-inflammatory response. The expression of genes that provide protection against stress at the cellular level is triggered by a transcription factor, NRF2 (nuclear factor [erythroid-derived 2] -like factor 2). The interaction between NFkB and NRF2 is mainly antagonistic. The search for new compounds that selectively activate the transcription factor NFkB and NRF2 remains an urgent task of modern medicine.
Single cell and whole genome amplification product validation of preimplantation genetic testing systems for monogenic disorders
Authors: Соловьёва Е.В. , Канбекова О.Р. , Жигалина Д.И. , Скрябин Н.А. , Минайчева Л.И.
Published: February 2021

В рамках подготовительных этапов преимплантационного тестирования 9 моногенных заболеваний (ПГT-М) методом гнездовой ПЦР проанализированы 144 локуса (STR и патогенные варианты)109 единичных клеток и 24 образцов продуктов полногеномной амплификации (ПГА) нескольких клеток.

Pre-examination single cell validation was performed for preimplantation genetic testing (PGT-M) for 9 monogenic disorders by nested PCR for STR and pathogenic variants. Totally 109 single cells and 24 WGA products (by MDA) were analyzed.
NGS for prenatal diagnosis of fetal anomalies
Authors: Янова Т.И. , Канивец И.В. , Коростелев С.А. , Пьянков Д.В. , Удалова В.Ю. , Горгишели К.В. , Киевская Ю.К.
Published: February 2021

Использование высокопроизводительного секвенирования в пренатальной диагностике позволило значительно увеличить выявляемость причин аномалий развития плода, определенных при УЗИ. Установление релевантного варианта является важным для постановки диагноза и оценки прогноза. Цель настоящей работы - определить распространенность и структуру моногенных заболеваний, являющихся причиной пороков развития плода при использовании секвенирования нового поколения (NGS). В нашем исследовании было проанализировано 60 образцов ДНК плодов, аномалии развития которых были выявлены при УЗИ во время беременности. Патогенные варианты, являющиеся причиной аномалий развития были найдены у 71% плодов.

The use of high-throughput sequencing in prenatal diagnostics has significantly increased the detection of the causes of fetal abnormalities identified by ultrasound. Establishing a relevant option is important for making a diagnosis and evaluating the prognosis. The purpose of this work is to determine the prevalence and structure of monogenic diseases that cause fetal malformations using next generation sequencing (NGS). In our study, we analyzed 60 samples of fetal DNA whose abnormalities were detected by ultrasound during pregnancy. Pathogenic variants were found in 71% of fetuses.
Genetic screening of newborns in Baku for hereditary hemoglobinopathies
Authors: Бабаев М.Ш. , Алиева К.А. , Мамедова Р.Ф.
Published: February 2021

Наследственные гемоглобинопатии, входят в группу заболеваний крови, называемых гемолитическими анемиями. В общем случае гемоглобинопатии развиваются из-за синтеза аномального гемоглобина. Гемолитические анемии встречаются примерно у 12% населения Земного шара. Данная статья посвящена результатам генетического скрининга новорожденных г. Баку с целью исследования частоты гемоглобинопатий. Обследованы 267 новорожденных, 143 мальчика и 124 девочки. Исследования проводились в 2018-2019 гг. методом изоэлектрофокусирования. В статье также изложены результаты молекулярной диагностики β-талассемии у новорожденных методом высокотемпературной аллель-специфической амплификации. По данным проведенного исследования наследственных гемоглобинопатий в сыворотке крови новорожденных гор. Баку установлено наличие структурно-аномальных гемоглобинов S и D и патологических аллелей генов α- и β-талассемий. Идентифицированы типы мутаций β-талассемий. Полученные результаты генетического скрининга позволят своевременно проводить лечение больных детей до проявления клиники заболевания.

Hereditary hemoglobinopathies are part of a group of blood diseases called hemolytic anemias. In general, hemoglobinopathy is the synthesis of abnormal hemoglobin. Hemolytic anemias occur in approximately 12% of the world’s population. This article is devoted to the results of genetic screening among newborns in Baku in order to study the frequency of hemoglobinopathies. The study involved 267 newborns. Among them are 143 boys and 124 girls. The studies were carried out in 2018-2019. To determine hemoglobinopathies among newborns, we used the method of iso-electrofocusing. The article also details the results of molecular diagnostics of the β-thalassemia gene in newborns by high-temperature allele-specific amplification. In Baku, the presence of pathological genes of α- and β-thalassemias and structurally abnormal hemoglobins S and D was established. Types of β-thalassemia mutations have been identified.
Analysis of the teaching of genetics for junior students of Sechenov university
Authors: Кузин С.М. , Чебышев Н.В. , Богомолов Д.В. , Беречикидзе И.А. , Сахарова Т.В. , Лазарева Ю.Б. , Ларина С.Н.
Published: February 2021

Развитие генетики оказывает все большее влияние на современную медицину. На основе достижений в области структурной, функциональной геномики и эпигенетики сформировались новые наиболее перспективные направления - таргетная терапия, персонализированная и регенеративная медицина. Появились принципиально новые возможности для диагностики, прогнозирования, профилактики и лечения широкого спектра заболеваний. Все возрастающая роль генетики предъявляет принципиально иные требования к ее преподаванию в медицинских ВУЗах с использованием новых педагогических методик. Простого увеличения количества часов в рамках предмета «Биология» уже недостаточно для подготовки специалистов, чья профессиональная деятельность напрямую связана с генетикой: онкологов, вирусологов, иммунологов и многих других.

The development of genetics has an increasing influence on modern medicine. Based on the achievements in the field of structural, functional genomics and epigenetics, new most promising directions have been formed - targeted therapy, personalized and regenerative medicine. There are fundamentally new opportunities for the diagnosis, prediction, prevention and treatment of a wide range of diseases. The increasing role of genetics imposes fundamentally different requirements for its teaching in medical Schools using new pedagogical methods. Simply increasing the number of academic hours in the subject “Biology” is no longer enough to train specialists whose professional activities are directly related to genetics: oncologists, virologists, immunologists, and many others.
Formation of motivation in teaching medical genetics as a factor in improving the quality of education
Authors: Ясакова Н.Т. , Максимова Ю.В. , Гарный В.Е. , Лисиченко О.В. , Хорошевская Я.А. , Волошина Т.В. , Сенцова А.Л. , Максимов В.Н.
Published: February 2021

Для оценки отношения разных групп населения к некоторым проблемам медицинской генетики была разработана анкета для анонимного анкетирования. Она состоит из 16 вопросов, касающихся разных генетических аспектов. Анкета прошла психологическую и социальную экспертную оценку. В 2009 и 2019 годах проведено анкетирование студентов первых курсов медицинского университета и одного факультета педагогического университета, а также в группах специалистов: клинических ординаторов разных специальностей первого года обучения, и педагогов, работающих с детьми, имеющими ограниченные возможности здоровья. Всего проанализировано в 2009 и 2019 годах 816 и 964 анкеты соответственно. Благодаря расширению программы преподавания медицинской генетики в медицинском университете и большой просветительской работе в средствах массовой информации за последние 10 лет существенно улучшилось понимание и отношение к проблемам, связанным с наследственными заболеваниями. Для дальнейшего улучшения ситуации необходимо расширить профессиональную подготовку специалистов, работающих с детьми с наследственной патологией. Совместные действия позволят индивидуализировать коррекционную работу и повысить ее эффективность.

To assess the attitude of different groups of the population to some problems of medical genetics, a questionnaire for anonymous survey was developed. It consists of 16 questions regarding various genetic aspects. The questionnaire passed a psychological and social expert assessment. In 2009 and 2019, a survey was conducted of first-year students of a medical university and one faculty of a pedagogical university, as well as in groups of specialists: clinical residents of different specialties in the first year of study, and teachers working with children with disabilities. A total of 816 and 964 questionnaires were analyzed in 2009 and 2019, respectively. Thanks to the expansion of the teaching of medical genetics at the medical university and extensive work with the media over the past 10 years, understanding of the problems associated with hereditary diseases has improved significantly. It is necessary to expand the professional training of specialists working with children with hereditary pathology to further improve the situation. Joint actions will make it possible to individualize corrective work and increase its effectiveness.
Partager son expérience et connaissances pour la dialyse à domicile
Authors: Verger Christian , Dratwa Max , Durand Pierre-Yves
Published: February 2021

Le besoin d’un accès facile, à la portée de tous, de l’information scientifique, a conduit ces dernières années à l’apparition de nombreuses revues directement consultables par Internet. La qualité de l’information est garantie dès lors que les articles qui y sont publiés sont soumis à l’analyse critique d’un comité de lecture par les experts reconnus dans son domaine. Dès 1995, le RDPLF a disposé du Bulletin de Dialyse Péritonéale (BDB) qui était publié 4 fois par an jusqu’au début des années 2000. Les anciens numéros peuvent encore être consultés sur son site. Néanmoins pour différentes raisons, dont les coûts d’impression nous avions dû ensuite restreindre l’édition de cette revue à tous les deux ans pour les résumés du Symposium.Nous avons pensé que l’heure était venue d’actualiser le recours à ce media sous une forme moderne : le libre accès par Internet (connu sous le terme « Open Access »). Depuis le RDPLF a évolué et est devenu en pratique RDPLF-HDD puisque nous incluons maintenant toutes les méthodes de domicile. Pour souligner cet aspect le nom de la revue est changé en Bulletin de Dialyse à Domicile (BDD).Le BDD sera publié en accès libre une fois par trimestre pour commencer et le rythme évoluera en fonction des besoins. Il comportera différentes sections : Editorial, Résultats du RDPLF, articles de formation pratique, articles cliniques d’intérêt général, etc..le but dans l’ensemble est d’occuper une place qui n’est pas forcément assurée par d’autres revues, délibérément tournée vers la pratique du domicile, ce qui n’exclue pas de temps en temps des travaux plus fondamentaux que des auteurs accepteraient de partager en accès libre.Les articles seront indexés par un numéro DOI. Nous souhaitons aussi par ce moyen, donner la possibilité à des néphrologues et infirmières qui le souhaitent les moyens d’une publication rapide de leurs travaux.Pour assurer la qualité des documents, ils seront tous soumis à un comité de lecture dont la composition est disponible sur ce site dans la rubrique a propos.C’est à vous, néphrologues et infirmières qui traitez des patients en dialyse à domicile, qu’il appartient, par vos futurs articles, d’assurer le succès de ce projet.Vous pouvez dès maintenant nous soumettre directement vos articles, soit en vous inscrivant sur le site, soit en nous les adressant pas mail ou courrier. Merci d'avance, pour nos patients, à tous ceux et celles qui nous aideront.
Implementing Religious Character Education by the Teacher on Sociology Learning in SMA YPK Pontianak
Authors: Megawati Megawati
Published: February 2021

This study would determine the implementation of character education of religious value by teacher in sociology learning in SMA YPK Pontianak and to find out the factors that influence the character education of religious values implementation in sociology learning in SMA YPK Pontianak. The method used in this study was descriptive method with qualitative approach. The data sources of the study were the students of grade X IIS and the sociology teacher of grade X IIS, interview and observation’s result. The results of this study showedthat the character education of religious value implementationin sociology learning involved greeting, praying before the class, the teacher implemented character education in the learning process by appreciating  diversity in religion, the teacher also taught about the religious dimensions, such individuals-to-individuals, individuals to God , individuals to environment relationships, religiousservices, praying after class. Factors that influence the implementation of the character education of religious values in SMA YPK Pontianak were the religious diversity and the inadequatefacilities of worship places.
Relasi Sosial Antar Pedagang Perempuan Di Pasar Tradisional Dahlia Sungai Jawi Pontianak
Authors: Yuyun Melina
Published: February 2021

This research was to find out the from of associative and dissociative social relations between female traders in traditional markets dahlia sungai jawi Pontianak. This research method was descriptive with a qualitative approach. The source of data of this research was the female traders in the traditional market of dahlia Sungai Jawi Pontianak and the data was a data sheet provided by the office of UKM trade of Pontianak and the results of interviews with female traders in the traditional market of dahlia Sungai Jawi Pontianak. The results showed that associative social relations in the from of bargaining among fellow female traders in the traditional market of dahlia Sungai Jawi Pontianak did not have any agreement between fellow traders and the from of exchange of goods and services in the from of many exchange. Assosiative social relations in the from of harmony could be seen, namely guarding the stalls of fellow traders, and helping to sell the merchandise among fellow traders. Dissosiative social relations in the from of economic competition was seen among fellow femaletraders in the traditional market of dahlia namely,competition of the quality of the new trade, competition for complete the merchandise, and competition of service
Penggunaan Metode Kooperatif Picture And Picture Guna Meningkatkan Kualitas Pembelajaran
Authors: Suryani Suryani
Published: February 2021

In the teaching and learning process of Social Sciences, many problems are carried out by the teacher. One of them is some students still rely on their teacher's activities in learning, so the role of students is still very lacking. Even thought, the teacher always involving students to play an active in learning process. In contrary, students assume that the subject of Social Sciences is very boring. So that this causes a low quality of learning in Social Sciences, especially in  SMP Negeri 21 Pontianak. This cases has tickled the writer to take the theme of research on picture and picture cooperative methods to improve the quality of learning and increase the interest and activity of students in teaching and learning activities in Social Sciences.As for the techniques used of data collection is through observation by teachers and collaborators. The research involved two cycles of 42 students. While the data analysis used description with percentage techniques. Furthermore the level of activity of students and teachers is expressed in the category of "very good", "good" or "moderate", while the success of using picture and picture cooperative methods is stated as "successful", "less successful", or "unsuccessful".The results of this reseacrh with picture and picture cooperative methods have been successful, which is showing indicated by an increase in student activity of learning because it has reached the specified criteria is 81% of students are actively involved, while teacher activities reach 86.53%, as well as student learning outcomes with a value of 78.57, where this shows that it is exceeding the KKM value of 72, with a percentage of completeness of 76%, which has exceeded the criteria of 75%.
PONTIANAK CHRISTIAN PAGUYUBAN EFFORTS IN MAINTAINING MECHANICAL SOCIAL SOLIDARITY OF MEMBERS
Authors: Trinugroho Agustinus Catur
Published: February 2021

The objective of this research was to know the effort of Paguyuban Kristen Jawi Pontianak maintain collective consciousness, religious ties and social ties. The research used descriptive method and qualitative approach. The techniques of data collection were observation, interview and the study of documentation. The research instruments were the directive of observation, directive of interview, and the documentation tools. This research showed that collective consciousness of the gemeinschaft is already. There is effort done maintain the collective consciousness as for mutually coordinate and worship priority.  This religious tie of the gemeinschaft is good enough. There is effort done maintain the religious tie is get the religous vacation on specific moments that run every year. Social tie of the gemeinschaft was good. There is effort done maintain the social tie as run with instilling tolerance to gemeinschaft members and set the schedule activity and then run that as consistance.
ANALISIS INTERAKSI SISWA DAN ORANG TUA YANG BEKERJA DI SMAN 1 MEMPAWAH
Authors: Apriani Heni
Published: February 2021

This study aims to know the role of interaction between students and their parents who work at SMAN 1 Mempawah. This research is conducted according to descriptive qualitative method. The technique used to collecting the data are direct observation, direct communication and documentary studies, while the instrumen are observasi, inteview and documentation. The analysis of this research presented according to descriptive qualitative by 10 participants that consisting of 2 people in each 5 families. The results showed that the interaction between students and their parents in social contact and communication are well established. The kids kiss their parents’ hand before go to school, and using their phone to communicate to their parents
Perubahan Sosial Terhadap Perkembangan Desa Rasau Jaya 3 Setelah Pembangunan Taman Bunga Rajati Flower Garden
Authors: Istiqomah Istiqomah , Novitasari Ragil Tri
Published: February 2021

The purpose of this study is for learning. This study entitled Social Change Towards Development of Rasau Jaya Village 3 After the Development of the Rajati Flower Garden. With the problem of how social change in the village of Rasau Jaya 3, economic improvement after the construction of a flower garden, development planning or the addition of facilities. This research method is a descriptive qualitative approach. Data sources of this research are primary data and secondary data. The results showed that: after the construction of the flower garden in Rasau Jaya 3 village the development of social change there was increasing, the people there accepted the development of the flower garden, because with the development of the community's economy there could be increased, because the people there could sell at around the flower garden so that it can increase their economy again there, and there will be plans to add facilities in the flower garden so that it can attract visitors to keep coming to the flower garden of the flower garden rajati.