bluuebunny/crossref_metadata_2025 huggingface dataset:
Biology and chemical weed management of Cynanchum acutum L.
Authors: Bar Oeri , Lati Ran Nisim , Schäckermann Jessica , Kapiluto Omer , Spodek Malkie , Gamliel Abraham , Matzrafi Maor
Published: September 2024

Cynanchum acutum L. (Asclepiadaceae) is a perennial vine weed widespread in the Mediterranean region. In Israel, it is native to areas such as the Golan Heights, Galilee, and the northern Negev. Recently, its range has expanded to the Arava Valley in the southern part of Israel. In this study, we investigate the biology and phenology of C. acutum plants originating from both native and invasive populations. To achieve this, we utilized rhizomes from the invasive area in the southern region characterized by a hyper-arid desert climate (Yahel), and from the northern region characterized by a temperate climate (Gvat). Additionally, we examine chemical-based weed management approaches for controlling C. acutum. Rhizomes collected from the northern region showed higher emergence rates at temperatures between 15-30°C, whereas at 35°C, rhizomes from the southern region exhibited a higher emergence rate. Phenology experiments conducted simultaneously at southern and northern locations revealed that plants from the northern location flowered first at both sites. However, flowering initiation occurred ~20 days earlier on average when plants from both populations were grown at the southern site. Pre-emergence herbicide treatments using indaziflam and pyroxasulfone were effective in inhibiting C. acutum root formation. According to the percentage of emergence, pendimethalin showed higher emergence (93% and 100%) in comparison to indaziflam (10% and 93%) and pyroxasulfone (72% and 70%) (2020 and 2021, respectively). Treatments including the herbicide fluroxypyr, as well as the treatment of glyphosate + saflufenacil + surfactant were highly effective in controlling C. acutum plants. These results offer valuable insights into effective weed management strategies for addressing C. acutum infestations. Moreover, our findings underscore the remarkable plasticity of this species and shed light on how populations originating from diverse habitats may have adapted to distinct environmental conditions.
LIF neuron —a memristive realization
Authors: Alammari Khalid , Heidarpur Moslem , Ahmadi Majid , Ahmadi Arash
Published: September 2024

This study introduces a pioneering design for leaky integrate-and-fire (LIF) neurons by integrating memristor devices with CMOS transistors, thereby forming an innovative hybrid CMOS/memristor neuron circuit. Employing Pt/TaOx/Ta as the memristor device, the proposed model was meticulously implemented and rigorously evaluated using the Cadence Virtuoso simulation environment. The simulation outcomes affirm the effective functionality of the design, marking a significant advancement in hybrid circuit engineering. Notably, the proposed neuron circuit exhibits a compact footprint, attributed to the efficient utilization of hybrid CMOS/memristor gates. This characteristic is poised to address the critical challenge of scaling in current neuromorphic systems, offering a viable pathway to substantially augment density and cater to the escalating demands of advanced computational architectures. The findings of this research hold promising implications for enhancing the efficiency and scalability of neuromorphic systems, setting a new benchmark for future developments in this domain.
An experimental verification system for electric vehicle and grid interactive charging safety
Authors: Han Jun , Fan Anjie , Cai Chao , Pan Wenjie , Chen Haofei
Published: September 2024

The development of electric vehicles (EVs) reduces dependence on fossil fuels, promotes energy conservation and emissions reduction, and facilitates the transition to clean energy sources in the power grid. However, subjective charging behavior among EV owners can lead to blind charging practices, compromising the reliability of the distribution network by widening the peak-to-valley difference. To address safety concerns during the charging process, this paper proposes hardware and software systems for an experimental verification system. The network architecture, focused on charging safety, is examined. Analysis of the system’s operation data reveals that it enables bidirectional interaction between electric vehicles and the power grid. This solution proves ef-fective for integrating a large number of EVs in peak-shaving and valley-filling efforts, laying a technical foundation for their inclusion in the power grid for peak shaving, valley filling, as well as providing standby and frequency regulation services.
Spatiotemporal variation of alpine gorge watershed landscape patterns via multi-scale metrics and optimal granularity analysis: a case study of Lushui City in Yunnan Province, China
Authors: Wang Yongshu , Yan Xiangdong , Fang Qingping , Wang Lan , Chen Dongbo , Yu Zhexiu
Published: September 2024

Introduction

The selection of an optimal scale or granularity in landscape analysis is pivotal for uncovering inherent patterns and changes driven by processes. Variations in spatial resolution can significantly alter the proportions and distributions of various landscape types, thereby impacting the assessment of landscape patterns. Despite its importance, the scale factor is frequently neglected in studies focusing on long-term landscape dynamics.

Methods

Bridging this gap, we utilized remote sensing imagery data from 1986 to 2020 for Lushui City, integrating remote sensing (RS) and geographic information system (GIS) technologies to generate land cover maps. Our focus centered on investigating the sensitivity of landscape pattern indices within the 30–1000m scale. Combining the first scale domain with an information loss assessment model, we identified the optimal granularity for the analysis, conducting a detailed spatiotemporal examination of landscape pattern from 1986 to 2020 using the index analysis method.

Results and discussion

The results show that: (1) The dominance of forests in Lushui City, yet reveal a significant increase in construction land area over the study period, primarily driven by the conversion of forest and grassland. (2) Among the 10 examined indices, four (PD, ED, TE, and LSI) demonstrated predictable responses to changes in granularity, while three (PAFEAC, COHESION, AI) exhibited unpredictable stepwise reactions. Three indices (LPI, SHDI, PLAND) displayed minimal regularity to granularity changes. (3) The optimal long-term landscape analysis granularity for Lushui was identified as 100 m. (4) Before 1996, the city’s landscape exhibited characteristics of aggregation, good connectivity, and minimal anthropogenic disturbance. However, post-1996, the landscape experienced disruptions, leading to an overall increase in fragmentation. The expansion of cultivated land and construction land due to urbanization has intensified landscape fragmentation. However, policies such as converting cropland to forest and planned ecological civilization initiatives have restored forest coverage and improved landscape cohesion and connectivity in Lushui City. This research offers vital insights for ecological planning and resource management in alpine valley watershed cities, deepening our grasp of landscape pattern evolution.
Current research and future prospects of immunonutrition in gastrointestinal malignancies
Authors: Ma Xiaoyan , Pei Beibei , Wu Na , Wang Chen , Yu Yanling , Yang Wenhui
Published: September 2024

Immune nutrition, as an integral component of nutritional support therapy, has garnered significant attention and research in the treatment of gastrointestinal malignancies. Recent advancements in nutritional formulas containing components such as glutamine, omega-3 polyunsaturated fatty acids, and arginine have led to the development of what is now termed immune nutrition or pharmacological nutrition. These formulations go beyond traditional nutritional support, functioning more like nutritional supplements with pharmacological effects. Patients with gastrointestinal malignancies often experience malnutrition and metabolic disturbances, resulting in immune dysfunction, cytokine dysregulation, and endocrine abnormalities. These issues can compromise intestinal mucosal barrier function, affecting the efficacy and prognosis of anticancer therapies. Recent studies indicate that immune nutrition can modulate specific mechanisms involved in various immune and inflammatory pathways, thereby improving patients’ immune status and treatment outcomes. While optimal patient selection, dosing, and timing of immune nutrition are still under investigation, its potential applications in oncology are promising. This article aims to analyze the existing evidence regarding the therapeutic benefits of immune nutrition in gastrointestinal malignancies, offering insights into its clinical standardization and application.
Structure characteristics and microwave dielectric properties of ZnZrNb2O8 oxide ceramics
Authors: Kumar Ashwini , Sharma Poorva , Qiu Fujun
Published: September 2024

This study investigates the synthesis, structural analysis, and microwave dielectric characteristics of ZnZrNb2O8 ceramics, prepared via solid-state reaction method and subjected to sintering at temperatures ranging from 1,000°C to 1,200°C for 4 h. X-ray diffraction (XRD) analysis confirms the successful formation of ZnZrNb2O8 phase, with a monoclinic wolframite phase. Scanning electron microscopy (SEM) investigations unveil microstructural features such as grain size and porosity, reveals material’s morphological details. Dielectric properties conducted in the microwave frequency regime show a correlation between dielectric constant (εr) and relative density of the ceramics. Importantly, the ceramics exhibited a suitable dielectric constant and low dielectric loss, indicative of their suitability for microwave applications. Remarkably, ZnZrNb2O8 ceramics sintered at 1,150°C for 4 h exhibit excellent microwave dielectric properties (εr = 27.2, Q × f = 54,500 GHz, and τf = −60 ppm/°C). These findings underscore the potential of ZnZrNb2O8 ceramics as advanced materials for high-frequency applications, including filters, resonators, and other microwave devices, thus contributing significantly to the advancement of next-generation telecommunications technologies.
Advances in improving tribological performance of titanium alloys and titanium matrix composites for biomedical applications: a critical review
Authors: Abakay Eray , Armağan Mustafa , Yıldıran Avcu Yasemin , Guney Mert , Yousif B. F. , Avcu Egemen
Published: September 2024

Titanium (Ti) alloys have been widely used in biomedical applications due to their superior mechanical, physical, and surface properties, while improving their tribological properties is critical to widening their biomedical applications in the current era. The present review examines the recent progress made in enhancing the tribological performance of titanium alloys and titanium matrix composites for biomedical purposes. It specifically focuses on the progress made in biomedical coatings, mechanical surface treatment, and developing titanium matrix composites in terms of their processing, tribological testing conditions, and characterization. Despite thorough investigations, the specific testing procedures for evaluating the friction and wear properties of the alloy and/or biomedical component are still uncertain. The majority of researchers have selected test methods and parameters based on previous studies or their own knowledge, but there is a scarcity of studies that incorporate limb-specific tribological tests that consider the distinct kinematic and biological structure of human limbs. Since advanced microscopy has great potential in this field, a variety of advanced characterization techniques have been used to reveal the relationship between microstructural and tribological properties. Many coating-based strategies have been developed using anodizing, PEO, VD, PVD, nitriding, thermal spray, sol-gel, and laser cladding, however; composition and processing parameters are crucial to improving tribological behaviour. Reinforcing component type, amount, and distribution has dominated Ti matrix composite research. Ti grade 2 and Ti6Al4V alloy has been the most widely used matrix, while various reinforcements, including TiC, Al2O3, TiB, hydroxyapatite, Si3N4, NbC, ZrO2 have been incorporated to enhance tribological performance of Ti matrix. Mechanical surface treatments improve biomedical Ti alloys’ tribological performance, which is advantageous due to their ease of application. The implementation of machine learning methods, such as artificial neural networks, regression, and fuzzy logic, is anticipated to make a substantial contribution to the field due to their ability to provide cost-effective and accurate results. The microstructural and surface features of biomedical Ti alloys directly affect their tribological properties, so image processing strategies using deep learning can help researchers optimize these properties for optimal performance.
A method for simultaneously measuring friction and gap at metal–lubricant interface by combined use of atomic force microscopy and line-and-space patterned metal films
Authors: Yamashita Naoki , Hirayama Tomoko
Published: September 2024

In boundary lubrication, adsorbed molecular films formed by lubricant additives on the metal surfaces of sliding parts effectively reduce friction and wear. A method is presented for simultaneously measuring friction and the gap at a metal–lubricant interface under boundary lubrication conditions using atomic force microscopy. In this method, line-and-space patterns are microfabricated in Cu films on Si substrates, and the gap is evaluated from the step height change when scanning in base oil and in base oil with an additive. Neutron reflectometry showed that whereas both stearic acid and stearyl alcohol formed molecular film about 2 nm thick on Cu film in a static state, the gap increased only with stearic acid due to maintaining a molecular film on the Cu film. This demonstrates the feasibility of the proposed method as means for visualizing the gap and shows that there is a difference between the two additives in their film-forming ability in a static state and their durability against friction. The proposed method for simultaneously measuring friction and the gap at a metal–lubricant interface is thus an effective way to investigate the tribological performance of additives under boundary lubrication conditions.
Conditional survival and changing risk profile in patients with gliosarcoma
Authors: Xu Lei , Yang Zhihao , Chen Huawei , Sun Chengjun , Tu Chuanjian , Gu Zhiwei , Luo Ming
Published: September 2024

Background

Conditional survival (CS) considers the duration since the initial diagnosis and can provide supplementary informative insights. Our objective was to evaluate CS among gliosarcoma (GSM) patients and develop a CS-incorporated nomogram to predict the conditional probability of survival.

Methods

This retrospective study using the Surveillance, Epidemiology, and End Results (SEER) database included patients with GSM between 2000 and 2017. The CS was defined as the probability of surviving additional y years after already surviving for x years. The formula utilized for CS was: CS(y|x) = S(y + x)/S(x), where S(x) denotes the overall survival at x years. Univariate Cox regression, best subset regression (BSR) and the least absolute shrinkage and selection operator (LASSO) were used for significant prognostic factors screening. Following this, backward stepwise multivariable Cox regression was utilized to refine predictor selection. Finally, a novel CS-integrated nomogram model was developed and we also employed diverse evaluation methods to assess its performance.

Results

This study included a total of 1,015 GSM patients, comprising 710 patients in training cohort and 305 patients in validation cohort. CS analysis indicated a gradual increase in the probability of achieving a 5-year survival, ascending from 5% at diagnosis to 13, 31, 56, and 74% with each subsequent year survived after 1, 2, 3, and 4 years post-diagnosis, respectively. Following variable screening through univariate Cox regression, BSR, and LASSO analysis, five factors-age, tumor stage, tumor size, radiotherapy, and chemotherapy-were ultimately identified for constructing the CS-nomogram model. The performance of the nomogram model was validated through discrimination and calibration assessments in both the training and validation cohorts. Furthermore, we confirmed that the effectiveness of the CS-nomogram in stratifying GSM patient risk status.

Conclusion

This nationwide study delineated the CS of patients diagnosed with GSM. Utilizing national data, a CS-nomogram could provide valuable guidance for patient counseling during follow-up and risk stratification.
The role of the hepatitis B virus genome and its integration in the hepatocellular carcinoma
Authors: Li Weiyang , Wang Suhao , Jin Yani , Mu Xiao , Guo Zhenzhen , Qiao Sen , Jiang Shulong , Liu Qingbin , Cui Xiaofang
Published: September 2024

The integration of Hepatitis B Virus (HBV) is now known to be closely associated with the occurrence of liver cancer and can impact the functionality of liver cells through multiple dimensions. However, despite the detailed understanding of the characteristics of HBV integration and the mechanisms involved, the subsequent effects on cellular function are still poorly understood in current research. This study first systematically discusses the relationship between HBV integration and the occurrence of liver cancer, and then analyzes the status of the viral genome produced by HBV replication, highlighting the close relationship and structure between double-stranded linear (DSL)-HBV DNA and the occurrence of viral integration. The integration of DSL-HBV DNA leads to a certain preference for HBV integration itself. Additionally, exploration of HBV integration hotspots reveals obvious hotspot areas of HBV integration on the human genome. Virus integration in these hotspot areas is often associated with the occurrence and development of liver cancer, and it has been determined that HBV integration can promote the occurrence of cancer by inducing genome instability and other aspects. Furthermore, a comprehensive study of viral integration explored the mechanisms of viral integration and the internal integration mode, discovering that HBV integration may form extrachromosomal DNA (ecDNA), which exists outside the chromosome and can integrate into the chromosome under certain conditions. The prospect of HBV integration as a biomarker was also probed, with the expectation that combining HBV integration research with CRISPR technology will vigorously promote the progress of HBV integration research in the future. In summary, exploring the characteristics and mechanisms in HBV integration holds significant importance for an in-depth comprehension of viral integration.
Dendrites contribute to the gradient of intrinsic timescales encompassing cortical and subcortical brain networks
Authors: Wu Kaichao , Gollo Leonardo L.
Published: September 2024

Introduction

Cytoarchitectonic studies have uncovered a correlation between higher levels of cortical hierarchy and reduced dendritic size. This hierarchical organization extends to the brain's timescales, revealing longer intrinsic timescales at higher hierarchical levels. However, estimating the contribution of single-neuron dendritic morphology to the hierarchy of timescales, which is typically characterized at a macroscopic level, remains challenging.

Method

Here we mapped the intrinsic timescales of six functional networks using functional magnetic resonance imaging (fMRI) data, and characterized the influence of neuronal dendritic size on intrinsic timescales of brain regions, utilizing a multicompartmental neuronal modeling approach based on digitally reconstructed neurons.

Results

The fMRI results revealed a hierarchy of intrinsic timescales encompassing both cortical and subcortical brain regions. The neuronal modeling indicated that neurons with larger dendritic structures exhibit shorter intrinsic timescales. Together these findings highlight the contribution of dendrites at the neuronal level to the hierarchy of intrinsic timescales at the whole-brain level.

Discussion

This study sheds light on the intricate relationship between neuronal structure, cytoarchitectonic maps, and the hierarchy of timescales in the brain.
Impact of the Promoting Physical Activity in Regional and Remote Cancer Survivors intervention on health-related quality of life in breast and colorectal cancer survivors
Authors: Hardcastle Sarah J. , Leyton-Román Marta , Maxwell-Smith Chloe , Hince Dana
Published: September 2024

Background

The PPARCS trial examined the efficacy of a distance-based wearable and health coaching intervention to increase physical activity (PA) in breast and colorectal cancer (CRC) survivors living in non-metropolitan areas. This paper examines the effects of the intervention on health-related quality of life (HRQoL) at 12 weeks (T2; end of intervention) and 24 weeks (T3; follow-up).

Methods

Participants that were insufficiently physically active and had successfully completed cancer treatment were randomised to an intervention or control group. PA was assessed using an ActiGraph (GT9X) at baseline, T2, and T3. Intervention effects on HRQoL were analysed using quantile regression comparing treatment groups across time.

Results

A total of 87 were randomised to intervention and control groups. There were generally no statistically significant differences between the groups on any HRQoL item except for pain. There was an arm (F(1, 219) = 5.0. p = 0.027) and time (F(2,221) = 4.8, p = 0.009) effect, reflecting the higher pain scores in the control group when collapsed across time points (median difference 16.7, CI 1.9 to 31.4, p = 0.027). For global HRQoL, the intervention group increased by 8.3 points between T1 and T2. The overall group median when collapsed across time was 16.7 points CI 8.2 to 25.2, p <0.001) greater in the intervention group than controls.

Conclusions

While the PPARCS intervention resulted in significant increases in PA, participants indicated a high HRQoL at baseline, leaving little room for improvement. Findings suggest that PA may improve global HRQoL and pain in breast and CRC survivors.
Case report: Staged tension-reducing excision of giant acquired vulvar lymphangioma secondary to cervical cancer surgery
Authors: Hu Ling-Juan , Fang Hao-Ming , Lin Huan-Mei , Kang Xu , Lin Ying , Xiao Jing
Published: September 2024

Introduction

Acquired vulvar lymphangioma (AVL), a rare disease caused by the dilation of superficial lymphatic vessels secondary to deep lymphatic vessel injury, is characterized by a wide range of morphological diversity and massive exudate. This morphological heterogeneity has often led to misdiagnosis or non-diagnosis. The management of AVL presents a therapeutic challenge due to the absence of a standardized treatment protocol.

Case presentation

A 53-year-old female patient, previously received surgical treatments for stage IIb cervical squamous cell carcinoma, presented with vulvar enlargement and copious amount of yellow exudate seven years post-treatment. Clinically, the patient exhibited chronic vulvar swelling, with easily-exudated nodules. The vulvar biopsy revealed lymphatic vessel dilation with lymphocyte infiltration, consistent with AVL. Due to the extensive lesions and severe exudate, staged excisions of bilateral vulvar lesions were performed at one-month intervals. Follow-up examinations of this patient for one-year post-surgery showed no evidence of recurrence.

Conclusion

In this instance, AVL manifest secondary to cervical cancer surgery, as a result of damage to the deep lymphatic vessels of the vulva, with characteristic symptoms of copious amounts of exudate and vulvar lesions with diverse morphologies, which provides a cautionary note for physicians. Besides, the staged resection strategy in this case may offer insights into surgical treatment protocol for extensive AVL.
Giant ovarian yolk sac tumor during late pregnancy: a case report and literature review
Authors: Wang Qin , Zuo Jianxin , Liu Chong , Zhou Huansheng , Wang Wenjie , Wang Yankui
Published: September 2024

The manifestation of a giant ovarian yolk sac tumor during late pregnancy is relatively rare. A yolk sac tumor is a highly malignant germ cell tumor that originates from primitive germ cells. It is characterized by yolk sac differentiation in vitro. The frequency of prenatal examinations should be appropriately increased for ovarian tumors discovered during pregnancy. Furthermore, regular follow-up ultrasound should be performed, and tumor markers should be dynamically detected. If needed, imaging examinations such as computed tomography and magnetic resonance imaging should be combined to comprehensively investigate disease progression. If the tumor diameter and tumor marker levels rapidly increase during pregnancy, the possibility of malignancy increases. Therefore, exploratory laparotomy should be immediately performed to further improve subsequent treatment modalities, early diagnosis, early treatment, and prognosis. Herein, we report the case of a 28-year-old pregnant woman whose pregnancy was terminated at 29 weeks and 5 days. She complained of lower abdominal pain for 2 days. A pelvic mass was detected for 1 week, accompanied by increased levels of tumor markers such as serum alpha-fetoprotein, cancer antigen 125, carbohydrate antigen 724, and human epididymis protein 4. Imaging revealed the presence of a pelvic mass. At 32 weeks and 3 days of pregnancy, a cesarean section was performed, with a transverse incision in the lower uterine segment. Furthermore, pelvic adhesiolysis, omentectomy, right adnexectomy, right pelvic lymph node dissection, and pelvic metastasis peritonectomy were performed. The postoperative pathological diagnosis was yolk sac tumors of the ovary (stage IIB). Postoperatively, a five-cycle chemotherapy regimen comprising bleomycin, etoposide, and cisplatin was administered. During postoperative follow-up, the patient’s general condition was noted to be good, with the newborn and pregnant women ultimately achieving good outcomes. We reviewed the relevant literature to increase clinical doctors’ understanding of ovarian malignancy during pregnancy, guide treatment selection, and facilitate early intervention for associated diseases.
Identification of a novel FERMT1 variant causing kindler syndrome and a review of the clinical and molecular genetic features in Chinese patients
Authors: Zhang Qiang , Yang Qi , Shen Fei , Wang Linlin , Luo Jingsi
Published: September 2024

Background

Kindler Syndrome (KS, OMIM #173650), a rare autosomal recessive genetic disorder, is characterized by a spectrum of symptoms such as cutaneous fragility, blistering, photosensitivity, and mucosal involvement. These symptoms result from variations in the FERMT1 gene (Fermitin family member 1, OMIM: 607900), encoding kindlin-1, an essential component of focal adhesions.

Objective

This study aims to ascertain the potential pathogenicity of a FERMT1 variant identified in a Chinese patient and to explore the phenotypic and molecular genetic characteristics of all reported cases of Kindler Syndrome in the Chinese population.

Methods

Whole-exome sequencing (WES) was performed on the patient to identify candidate variants associated with KS, and Sanger sequencing was utilized to authenticate their presence and origin. To further assess the potential impact of these genetic variants, we employed a variety of in silico prediction tools. Concurrently, a review of various databases was undertaken to ascertain and consolidate information regarding cases of KS in Chinese families.

Results

We identified a novel likely pathogenic frameshift variant in the FERMT1 gene, specifically c.567_579delTATATATGACCCC (p.Ile190Serfs*10). The clinical presentation of this patient aligns with the diagnostic criteria for KS. The literature review reveals that the core clinical features of KS reported in the Chinese population include skin abnormalities (100%), as well as hyperkeratosis of the palms and soles (91.70%). Other clinical phenotypes encompass nail abnormalities (77.78%), abnormalities of the fingers/toes (75.00%), oral damage (70.00%), eye abnormalities (57.14%), and constipation (50.00%).

Conclusion

Our study enriches the genetic landscape of KS in the Chinese population and augments the understanding of phenotypic variability resulting from FERMT1 gene variants. The findings hold considerable significance for refining variant-based screening, genetic diagnosis, and comprehending the molecular pathogenesis underlying FERMT1-related disorders.
Exploring the potent enhancement effects of plyometric training on vertical jumping and sprinting ability in sports individuals
Authors: Xie Lin , Chen Jiayong , Dai Jing , Zhang Wenfeng , Chen Lunxin , Sun Jian , Gao Xiang , Song Junyi , Shen Hailun
Published: September 2024

Objective

This meta-analysis examines the impact of different combinations of plyometric training (complexity, training volume, and rest intervals) on immediate vertical jump and sprint performance in athletes.

Methods

A systematic search was conducted in four databases, and Cochrane guidelines were used to evaluate the quality of included studies. Review Manager 5.4 software was employed to analyze outcome measures. Nineteen randomized controlled trials involving 293 participants were included.

Results

Single plyometric training-induced post-activation potentiation (PAP) had a slight positive effect on vertical jump performance [SMD = −0.24, 95% CI (−0.38, −0.1), P = 0.0009]. Optimal results were observed with rest intervals of 0.3–4 min (SMD = 0.30, P = 0.0008). Sprint performance showed slight improvement [SMD = 0.27, 95% CI (0.03, 0.52), P = 0.03]. Complex plyometric training had a moderate effect on vertical jump performance [SMD = 0.58, 95% CI (−0.86, −0.23), P = 0.002], with the best outcomes seen with rest intervals exceeding 8 min (SMD = 0.77). Sprint performance also improved significantly [SMD = 0.8, 95% CI (0.01, 1.59), P = 0.05]. Single-session plyometric training did not significantly enhance vertical jump performance [SMD = −0.19, 95% CI (−0.41, −0.02), P = 0.07], but had a notable effect on sprint performance [SMD = 0.8, 95% CI (0.01, 1.59), P = 0.05], particularly with rest intervals exceeding 8 min (SMD = 0.77). Multiple-session plyometric training improved vertical jump (SMD = 0.43, 95% CI [0.01, 1.59), P = 0.00001 < 0.05], with optimal effects observed at rest intervals of 5–7 min (SMD = 0.64). Sprint performance also improved [SMD = 0.46, 95% CI (0.01, 0.81), P = 0.01 < 0.05].

Conclusion

Plyometric training as an activation method has significant enhancing effects, depending on training complexity, volume, and rest intervals.
Regulatory effects of tea polysaccharides on hepatic inflammation, gut microbiota dysbiosis, and serum metabolomic signatures in beef cattle under heat stress
Authors: Li Fan , Xu Jun , Xie Min , Fei Dan , Zhou Yaomin , Li Xiong , Guang Yelan , Gong Lihui , Hu Lizhen , Feng Fan
Published: September 2024

Background

Long-term heat stress (HS) severely restricts the growth performance of beef cattle and causes various health problems. The gut microbiota plays a crucial role in HS-associated inflammation and immune stress involving lymphocyte function. This study investigated the effects of dietary tea polysaccharide (TPS), a natural acidic glycoprotein, on HS-induced anorexia, inflammation, and gut microbiota dysbiosis in Simmental beef cattle.

Methods

The cattle were divided into two groups, receiving either normal chow or normal chow plus TPS (8 g/kg, 0.8%). Transcriptome sequencing analysis was used to analysis the differential signaling pathway of liver tissue. 16S rDNA sequencing was performed to analysis gut microbiota of beef cattle. Serum metabolite components were detected by untargeted metabolomics analysis.

Results

Hepatic transcriptomics analysis revealed that differentially expressed genes in TPS-fed cattle were primarily enriched in immune processes and lymphocyte activation. TPS administration significantly reduced the expression of the TLR4/NF-κB inflammatory signaling pathway, alleviating HS-induced hepatic inflammation. Gut microbiota analysis revealed that TPS improved intestinal homeostasis in HS-affected cattle by increasing bacterial diversity and increasing the relative abundances of Akkermansia and Alistipes while decreasing the Firmicutes-to-Bacteroidetes ratio and the abundance of Agathobacter. Liquid chromatography-tandem mass spectrometry (LC‒MS/MS) analysis indicated that TPS significantly increased the levels of long-chain fatty acids, including stearic acid, linolenic acid, arachidonic acid, and adrenic acid, in the serum of cattle.

Conclusion

These findings suggest that long-term consumption of tea polysaccharides can ameliorate heat stress-induced hepatic inflammation and gut microbiota dysbiosis in beef cattle, suggesting a possible liver-gut axis mechanism to mitigate heat stress.
Impact of irrigation, nitrogen fertilization, and plant density on stay-green and its effects on agronomic traits in maize
Authors: Chibane Nadia , Revilla Pedro , Yannam Venkata Rami Reddy , Marcet Purificación , Covelo Emma Fernández , Ordás Bernardo
Published: September 2024

Introduction

The stay-green (SG) or delayed leaf senescence enables crop plants to maintain their green leaves and photosynthetic capacity for a longer time after flowering. It is considered an important trait in maize breeding, which has contributed to gain in grain yield of modern varieties. It has been also used to improve the tolerance to drought and deficiencies in nitrogen fertilization (NF). However, the objective of this study is to evaluate the influence of water irrigation (WI), NF, and plant density (PD) on SG and the effect of SG on agronomic traits in maize.

Methods

Four SG lines and four non–stay-green (NSG) lines were evaluated in four contrasting environments under two WI, three NF, and two PD levels.

Results and discussion

As expected, the chlorophyll content of leaves at 45 days after flowering (Chlo45) was, on average, higher in the SG group of lines. The difference in Chlo45 between the SG and NSG genotypes was consistent across WI, NF, and PD and the environments. This is indicative that internal or developmental factors were more important than external signals in controlling the senescence. The effect of SG increasing thousand-kernel weight, stover yield at harvest, or moisture was not influenced by WI, NF, or PD but was altered by the background environment. Our results have implications for the application of SG as a secondary trait for enhancing abiotic stress tolerance. Future studies could consider a wider range of environmental conditions to assess the performance of SG traits under different climatic and soil conditions.
Stability of gross primary productivity and its sensitivity to climate variability in China
Authors: Xu Xiaojuan , Jiao Fusheng , Liu Jing , Ma Jie , Lin Dayi , Gong Haibo , Yang Yue , Lin Naifeng , Wu Qian , Zhu Yingying , Qiu Jie , Zhang Kun , Zou Changxin
Published: September 2024

Identifying the stability and sensitivity of land ecosystems to climate change is vital for exploring nature-based solutions. However, the underlying mechanisms governing ecosystem stability and sensitivity, especially in regions with overlapping ecological projects, remain unclear. based on Mann-Kendall, stability analysis method, and multiple regression method, this study quantified the stability and sensitivity of gross primary productivity (GPP) to climate variables [temperature, vapor pressure deficit (VPD), soil moisture, and radiation] in China from 1982 to 2019. Our findings revealed the following: (1) GPP demonstrated an increased trend with lower stability in Eastern regions, whereas a decreasing trend with higher stability was observed in Western and Southwest China. Notably, the stability of GPP was highest (74.58%) in areas with five overlapping ecological projects: Grain to Green, Natural Forest Resource Protection Project, Three-River Ecological Conservation and Restoration Project, Return Grazing to Grassland Project, and Three-North Shelter Forestation Project. (2) In regions with minimal or no overlapping ecological projects, temperature and radiation jointly dominated GPP variations. In contrast, water-related factors (VPD and soil moisture) significantly affected GPP in areas with multiple overlapping ecological projects. (3) In the southwestern and northeastern regions, GPP exhibited the highest sensitivity to climate change, whereas, in the eastern coastal areas and Tibet, GPP showed low sensitivity to climate change. In the Loess Plateau, where five ecological projects overlap extensively, carbon sinks primarily demonstrate a monotonic increasing trend, high stability, and low sensitivity to climate change. This study aimed to assess the stability of the land ecosystems and delineate their sensitivity to climate changes, thereby laying the groundwork for understanding ecosystem resilience.
Root-based inorganic carbon uptake increases the growth of Arabidopsis thaliana and changes transporter expression and nitrogen and sulfur metabolism
Authors: Gamarra Reinoso Liesel , Majláth Imre , Dernovics Mihály , Fábián Attila , Jose Jeny , Jampoh Emmanuel Asante , Hamow Kamirán Áron , Soós Vilmos , Sági László , Éva Csaba
Published: September 2024

Root-based uptake of inorganic carbon has been suggested as an additional carbon source. Our study aimed to characterize and understand the root-based uptake and fixation mechanisms and their impact on plant growth. 13C-labeled bicarbonate fed to Arabidopsis roots was assimilated into aspartic acid but mainly into sucrose, indicating that the added inorganic carbon was transported to the leaves. A hydroponic treatment was also established for A. thaliana using 2 mM NaHCO3 at pH 5.6, which enhanced the photosynthetic and growth parameters. According to transcriptome sequencing data, the observed enhancement in growth may be orchestrated by trehalose-6-phosphate signaling and supported by augmented nitrogen and sulfur assimilation. The analysis also revealed regulatory and transporter activities, including several nitrate (NRT2.1), and sulfate transporter (SULTR1;1 and SULTR1;2) candidates that could participate in bicarbonate uptake. Different transporters and carbon fixation mutants were assessed. Arabidopsis homologs of SLOW-TYPE ANION CHANNEL 1 (slah3) CARBONIC ANHYDRASE (βca4), and SULFATE TRANSPORTER (sultr1;2) mutants were shown to be inferior to the bicarbonate-treated wild types in several growth and root ultrastructural parameters. Besides, aquaporin genes PIP1;3 and PIP2;6 could play a negative role in the carbon uptake by venting carbon dioxide out of the plant. The findings support the hypothesis that the inorganic carbon is taken up by the root anion channels, mostly transported up to the shoots by the xylem, and fixed there by RuBisCo after the conversion to CO2 by carbonic anhydrases. The process boosts photosynthesis and growth by providing an extra carbon supply.
Legal regulation of surrogacy parentage determination in China
Authors: You Wenting , Feng Jun
Published: September 2024

Unlike natural conception and other assisted reproductive technologies, surrogacy involves three crucial factors: family legislation, family ethics, and reproductive technology. This makes the determination of parentage in surrogacy more complex. In China, surrogacy is completely prohibited by law. However, this prohibition has not diminished the interest in discussions around the family ethics, order, and relationships affected by surrogacy. In practice, disputes over parentage and child custody arising from surrogacy urgently need resolution through judicial practice. The current legal framework in China lacks clear regulations to address the complexities of surrogacy, leading to numerous unresolved disputes. To address this issue, it is advisable for China to enact clear legislative measures to govern parent–child relationships in surrogacy cases. This paper presents legislative recommendations for regulating surrogacy in China, with the hope that the judicial interpretations of the Supreme People’s Court of China can provide clear legal regulations on surrogacy during revisions.
Area of Focus in 3D Volumetry and Botulinum Toxin A Injection for Giant Diaphragmatic Hernia with Loss of Domain: A Case Report with Video Illustration
Authors: Nachtergaele Sylvie , Khalil Haitham , Martre Paul , Baste Jean-Marc , Roussel Edouard
Published: September 2024

Background

Chronic giant diaphragmatic hernia is a severe disease with challenging diagnosis and treatment. Given the risk of loss of domain, the use of botulinum toxin A is an option but has been minimally studied in diaphragmatic hernia surgery.

Case Report

We present a case of a giant diaphragmatic hernia in a 66-years-old patient who showed a 12-year history of progressive chronic respiratory insufficiency. There were not notion of traumatic injuries. The CT-scan showed a giant diaphragmatic hernia with herniation of small bowel, right liver, omentum and transverse colon.

Method

We assessed the risk of loss of domain using a 3D volumetry based on the Sabbagh score and decided to use Botox injection before laparoscopic reduction of the hernia due to the high risk of complications related to the loss of domain. A computed tomography was performed 24 months after surgery and showed no evidence of recurrence. The patient presented an excellent functional result with a normal physical activity.

Conclusion

This report is among the first to highlight the utility of 3D reconstruction in assessing the risk associated with loss of domain and in preparing the abdominal wall with botulinum toxin A for diaphragmatic hernia repair.
Efficient Absorbance-Based Assay for Rapid Antibiotic Susceptibility Testing of Enterobacterales
Authors: Axelsson Carolina , Nilson Bo , Rehnstam-Holm Ann-Sofi
Published: September 2024

It is increasingly important to rapidly receive information on the antimicrobial susceptibility of bacteria due to the rise in antimicrobial resistance worldwide. However, traditional phenotypic methods are time-consuming. Thus, the objective of this study was to develop a rapid assay that can detect antibiotic-resistant bacterial isolates phenotypically in less than 2 h. The microplate assay used in this study is based on absorbance measurements of pure bacterial isolates grown in liquid media with and without antibiotics. Absorbance was measured at the beginning of the assay and after 90 min of incubation at 37 °C. Susceptibility was calculated for bacterial isolates that, in the absence of antibiotics, exhibited more than a 50% growth increase by comparing the absorbance value of the culture in the presence of an antibiotic at 90 min with its initial value. Here, we show that it is possible to phenotypically screen the antibiotic susceptibility of Enterobacterales and Acinetobacter spp. isolates to three different antibiotics in 90 min. The test demonstrated an accuracy of 98.8%, sensitivity of 97.6%, and specificity of 99.6%. The false susceptibility rates were 0.2% and false resistance rates were 1.0%. This rapid and simple absorbance test has proven suitable for the screening of antibiotic susceptibility for a large number of strains with high accuracy and sensitivity. This method can be performed without specialized and costly materials and/or equipment, which makes it highly suitable for laboratories with limited resources.
Real-Life Outcomes of Adjuvant Targeted Therapy and Anti-PD1 Agents in Stage III/IV Resected Melanoma
Authors: Roccuzzo Gabriele , Fava Paolo , Astrua Chiara , Brizio Matteo Giovanni , Cavaliere Giovanni , Bongiovanni Eleonora , Santaniello Umberto , Carpentieri Giulia , Cangiolosi Luca , Brondino Camilla , Pala Valentina , Ribero Simone , Quaglino Pietro
Published: September 2024

This study was carried out at the Dermatologic Clinic of the University of Turin, Italy, to assess the effectiveness and safety of adjuvant therapy in patients who received either targeted therapy (TT: dabrafenib + trametinib) or immunotherapy (IT: nivolumab or pembrolizumab) for up to 12 months. A total of 163 patients participated, including 147 with stage III and 19 with stage IV with no evidence of disease. The primary outcomes were relapse-free survival (RFS), distant metastasis-free survival (DMFS), and overall survival (OS). At 48 months, both TT and IT approaches yielded comparable outcomes in terms of RFS (55.6–55.4%, p = 0.532), DMFS (58.2–59.8%, p = 0.761), and OS (62.4–69.5%, p = 0.889). Whilst temporary therapy suspension was more common among TT-treated patients compared to IT-treated individuals, therapy discontinuation due to adverse events occurred at comparable rates in both groups. Predictors of relapse included mitoses, lymphovascular invasion, ulceration, and positive sentinel lymph nodes. Overall, the proportion of BRAF-mutated patients receiving IT stood at 7.4%, lower than what was observed in clinical trials.
Fecundity and Nutrient Deficiency Following Obesity Treatment: Implications for Young-Onset Cancer Risk in Offspring
Authors: Barreto Savio George , Moy Chris , Pandol Stephen J. , Kow Lilian
Published: September 2024

Young-onset adult cancers have been an emerging problem over the last three decades in Australia [...]
Research on Markov Decision Model Predictive Control of Interior Permanent Magnet Synchronous Motor Based on Lumped Disturbances Compensation
Authors: Teng Yongxiao , Gao Qiang , Chen Xuehan , Xu Dianguo
Published: September 2024

To improve the performance of the interior permanent magnet synchronous motor control system, a Markov decision model predictive current control strategy based on a lumped disturbances sliding mode disturbance observer is proposed in this paper. A fast terminal sliding mode disturbance observer based on a recursive integral sliding surface is designed to observe and compensate the unideal factors in the motor control system unified as lumped disturbances. Then, according to the characteristic of model predictive control where only the first vector in the optimal control sequence is selected and applied to the system during rolling optimization, the discounted cost criterion based on the Markov decision process is introduced to enhance the control performance of the system. The compensation of lumped disturbances can eliminate the impact of unideal factors, enhance the dynamic performance of the speed loop, and eliminate the static errors in the current loop. The introduction of the discounted cost criterion can reduce the fluctuations in system states without affecting the system’s dynamic performance. Moreover, the proposed control strategy does not require the original control structure of the system to be changed. Experiments are carried out to verify the effectiveness of the proposed method.