bluuebunny/crossref_metadata_2025 huggingface dataset:
Intimate Partner Violence, Depression, and Anxiety Are Associated With Higher Perceived Stress Among Both Young Men and Women in Soweto and Durban, South Africa
Authors: Pakhomova Tatiana E. , Dietrich Janan Janine , Closson Kalysha , Smit Jenni , Hornschuh Stefanie , Smith Patricia , Beksinska Mags , Ndung'u Thumbi , Brockman Mark , Gray Glenda , Kaida Angela
Published: March 2021

Objectives: Psychological stress is an important determinant of health, including for mental well-being and sexual health. However, little is known about the prevalence and psychosocial and sexual health correlates of perceived stress among young people in South Africa, where elevated life-stressors are an important driver of health inequities. This study examines the association between intimate partner violence (IPV), psychosocial and sexual health, and perceived stress, by gender, among South African adolescents and young adults.

Methods: Using baseline survey data from AYAZAZI, a cohort study enrolling youth (16–24 years) from Durban and Soweto, we used the 10-item Perceived Stress Scale (PSS-10) to measure the degree to which an individual perceives their life situations as unpredictable, uncontrollable, and overloaded. Possible scores range between 0 and 40; higher scores indicating higher perceived stress. Crude and adjusted gender-stratified linear regression models examined associations between sexual health factors, experiences (young women) and perpetration (young men) of IPV, anxiety (APA 3-item Scale, ≥2 = probable anxiety), and depression (10-item CES-D Scale, ≥10 = probable depression) and perceived stress. Multivariable models adjusted for age, income, sexual orientation, and financial dependents.

Results: Of the 425 AYAZAZI participants, 60% were young women. At baseline, 71.5% were students//learners and 77.2% earned ≤ ZAR1600 per month (~$100 USD). The PSS-10 had moderate reliability (α = 0.70 for young women, 0.64 for young men). Young women reported significantly higher mean PSS scores than young men [18.3 (6.3) vs. 16.4 (6.0)]. In adjusted linear regression models, among young women experiences of IPV (β = 4.33; 95% CI: 1.9, 6.8), probable depression (β = 6.63; 95% CI: 5.2, 8.1), and probable anxiety (β = 5.2; 95% CI: 3.6, 6.8) were significantly associated with higher PSS scores. Among young men, ever perpetrating IPV (β = 2.95; 95% CI: 0.3, 5.6), probable depression (β = 6; 95% CI: 4.3, 7.6), and probable anxiety (β = 3.9; 95% CI: 2.1, 5.8) were significantly associated with higher perceived stress.

Conclusion: We found that probable depression, anxiety, perpetration of IPV among young men, and experiences of IPV among young women, were associated with higher perceived stress. Critical efforts are needed to address the gendered stressors of young men and women and implement services to address mental health within violence prevention efforts.
Evidence-Based African Swine Fever Policies: Do We Address Virus and Host Adequately?
Authors: Busch Frank , Haumont Céline , Penrith Mary-Louise , Laddomada Alberto , Dietze Klaas , Globig Anja , Guberti Vittorio , Zani Laura , Depner Klaus
Published: March 2021

African swine fever (ASF) is one of the most threatening diseases for the pig farming sector worldwide. Prevention, control and eradication remain a challenge, especially in the absence of an effective vaccine or cure and despite the relatively low contagiousness of this pathogen in contrast to Classical Swine Fever or Foot and Mouth disease, for example. Usually lethal in pigs and wild boar, this viral transboundary animal disease has the potential to significantly disrupt global trade and threaten food security. This paper outlines the importance of a disease-specific legal framework, based on the latest scientific evidence in order to improve ASF control. It compares the legal basis for ASF control in a number of pig-producing regions globally, considering diverse production systems, taking into account current scientific evidence in relation to ASF spread and control. We argue that blanket policies that do not take into account disease-relevant characteristics of a biological agent, nor the specifics under which the host species are kept, can hamper disease control efforts and may prove disproportionate.
EGFR and KRAS Mutations in Lung Parenchyma of Subjects With EGFR/KRAS Wild-Type Lung Adenocarcinoma
Authors: Chalela Roberto , González-García Jose Gregorio , Khilzi Karys , Curull Víctor , Sánchez-Font Albert , Longarón Raquel , Rodrigo-Calvo María Teresa , Martín-Ontiyuelo Clara , Gea Joaquim , Bellosillo Beatríz
Published: March 2021

The acquisition of driver mutations in non-tumoral cells appears to be very important during the carcinogenesis of adenocarcinoma (ADC). Recent studies suggest that cancer-related mutations may not necessarily be present only in malignant cells, but also in histologically “healthy cells”.

Objective: to demonstrate the presence of EGFR or KRAS mutations in non-tumoral lung cells in subjects with ADC and negative mutational status.

Results: mutations in EGFR or KRAS oncogenes were identified in the normal lung in 9.7% of the subjects. Exon 21 substitution L858R in EGFR was detected in two cases while the exon 19 deletion E746-A750 in the EGFR, the G12C and G12D substitutions in the KRAS were detected once. One patient presented three different mutations in the normal lung parenchyma (EGFR_L858R, KRAS_G12C and KRAS_G12D). The negative-mutation status of the tumor and the mutations detected in the “normal lung” were confirmed using highly sensitive and specific TaqMan PCR (CAST-PCR). No differences were found in terms of progression, progression-free survival or overall survival during the 18 months follow-up.

Conclusions: These results confirm the presence of driver mutations in the histologically normal lung parenchyma cells in the absence of mutations coexisting with the primary tumor.
A Case Study on Soundscape Analysis for the Historical and Ethnic Village of Dong Nationality in Zhaoxing County
Authors: Deng Zhiyong , Dong Kexin , Bai Danfeng , Tong Kaicheng , Liu Aili
Published: March 2021

A soundscape is a sound environment of the awareness of auditory perception and social or cultural understandings. Based on a soundscape investigation in 2019 in the historical and ethnic village of Dong Nationality in Zhaoxing County, Guizhou Province of China, a case study on the soundscape analysis with the acoustical sound pressure level and an impressive sound event or soundmark is introduced in this paper. Furthermore, in order to determine the subjective soundscape experience and its influence by the length of background music listening, the independent variable “Length of Listening” and six adjective pairs, such as “Monotonous” to “Rich”, “Clamorous” to “Quiet”, “Stressing” to “Relaxing”, “Boring” to “Vivid”, “Noisy” to “Musical” and “Disliked” to “Preferable” are chosen to obtain a curve-fit, which shows that the length of the music listening background has a higher correlation to the subjective experience, and no sufficient attention has been paid to the context of the traditional soundscape preservation, ethnic music and quiet and soft ambient sounds.
Qualitative Analysis of Lambda-Cyhalothrin on Chinese Cabbage Using Mid-Infrared Spectroscopy Combined with Fuzzy Feature Extraction Algorithms
Authors: Shen Yanjun , Wu Xiaohong , Wu Bin , Tan Yang , Liu Jinmao
Published: March 2021

Excess pesticide residues on cabbage are harmful to humans. In this study, we propose an innovative strategy for a quick and nondestructive qualitative test of lambda-cyhalothrin residues on Chinese cabbage. Spectral profiles of Chinese cabbage leaf samples with different concentrations of surface residues of lambda-cyhalothrin were collected with an Agilent Cary 630 FTIR Spectrometer. Standard normal variate (SNV), multiplicative scatter correlation (MSC), and principle component analysis (PCA) were utilized to preprocess the spectra. Then, fuzzy Foley-Sammon transformation (FFST), fuzzy linear discriminant analysis (FLDA), and fuzzy uncorrelated discriminant transformation (FUDT) were employed to extract features from the spectra data. Finally, k-nearest neighbor (kNN) was applied to classify samples according to the concentration of lambda-cyhalothrin residue. The highest identification accuracy rates of FFST, FLDA, and FUDT were 100%, 97.22%, and 100%, respectively. FUDT performed the best considering the combination of accuracy rate and required computing time. We believe that mid-infrared spectroscopy combined with fuzzy uncorrelated discriminant analysis is an effective method to accurately and quickly conduct qualitative analyses of lambda-cyhalothrin residues on Chinese cabbages. This method may have applications in other crops and other pesticide residues.
Evaluation of Leaf Rust Resistance in the Spanish Core Collection of Tetraploid Wheat Landraces and Association with Ecogeographical Variables
Authors: Martínez-Moreno Fernando , Giraldo Patricia , Cátedra María del Mar , Ruiz Magdalena
Published: March 2021

Spain has a great landrace diversity of the subspecies of the tetraploid species Triticum turgidum L., namely, durum (or durum wheat), turgidum (or rivet wheat) and dicoccon (or domesticated emmer wheat). These wheats have to confront several foliar diseases such as the leaf rust. In this work, a core collection of 94 landraces of tetraploid wheats were inoculated with three leaf rust isolates. Besides, a larger collection (of 192 accessions) was evaluated in the field. Although the majority of landraces were susceptible, approximately 20% were resistant, especially domesticated emmer wheat landraces. Several variables, such as late heading and red coat seeds were associated to resistant accessions. Regarding ecogeographic variables, a higher rainfall from October to February and more uniform temperature were found in the area of origin of resistant landraces. Based on these results, several resistant landraces were identified that potentially may be used in durum wheat breeding programs. In addition, a predictive model was elaborated to develop smaller subsets for future screening with a higher hit rate for rust resistance.
Examining the Influence of Sludge from Municipal Wastewater Treatment Plants Processed by Euphore Installations on the Quantity and Quality of Rapeseed and Soybean Production
Authors: Chiorescu Esmeralda , Filipov Feodor
Published: March 2021

Sludge management is a complex issue due to the environmental standards. It is required that the wastewater treatment activity be in close connection with the controlled recovery and storage of sludge. Thus, by using sludge in agriculture, nutrients essential for plant development can be recycled and some soil properties can be improved. The purpose of this paper was to present some results on the influence of municipal sludge treated and processed in a Euphore plant on the quantity and quality of rapeseed and soybean production. This technology allows for the recovery of the constituents of phosphorus, nitrogen, potassium, sulfur, magnesium, calcium, manganese, zinc, and copper. Our experimental data revealed that the obtained yield of Pioneer PT225 rapeseed hybrid was 5200 kg/ha in the variant treated with the Euphore process compared to that of the control variant at only 2356 kg/ha. For the Condor variety soybean crop, the highest average number of pods obtained per plant was 195.3 compared 88 pods per plant in the control variant. Thus, the sludge obtained from urban wastewater treatment plants processed using the Euphore method is a good source of macro and micronutrients in agriculture, without having a negative impact on the environment.
IgE-Binding Epitopes of Pis v 1, Pis v 2 and Pis v 3, the Pistachio (Pistacia vera) Seed Allergens
Authors: Barre Annick , Nguyen Christophe , Granier Claude , Benoist Hervé , Rougé Pierre
Published: March 2021

Sequential IgE-binding epitopes were identified on the molecular surface of the Pis v 1 (2S albumin), Pis v 2 (11S globulin/legumin) and Pis v 3 (7S globulin/vicilin)—major allergens from pistachio (Pistacia vera) seeds—using the Spot technique. They essentially consist of hydrophilic and electropositively charged residues well exposed on the surface of the allergens. Most of the epitopic regions identified on Pis v 1 and Pis v 3 do not coincide with the putative N-glycosylation sites and thus are not considered as glycotopes. Surface analysis of these epitopic regions indicates a high degree of conformational similarity with the previously identified epitopic regions of the corresponding allergens Ana o 1 (vicilin), Ana o 2 (legumin) and Ana o 3 (2S albumin) from the cashew (Anacardium occidentale) nut. These results offer a molecular basis for the IgE-binding cross-reactivity often observed between pistachio and cashew nut. They support the recommendation for prescribing pistachio avoidance in cashew allergic patients. Other conformational similarities were identified with the corresponding allergens Ses i 1 (2S albumin), Ses i 3 (vicilin) and Ses i 6 (legumin) from sesame (Sesamum indicum), and Jug r 1 (2S albumin), Jug r 2 (vicilin) and Jug r 4 (legumin) from walnut (Juglans regia). Conversely, conformation of most of the epitopic regions of the pistachio allergens often differs from that of epitopes occurring on the molecular surface of the corresponding Ara h 1 (vicilin), Ara h 2 (2S albumin) and Ara h 3 (legumin) allergens from peanut (Arachis hypogaea).
Analysis of Acetylcholinesterase Activity in Cell Membrane Microarrays of Brain Areas as a Screening Tool to Identify Tissue Specific Inhibitors
Authors: Rienda Bárbara , Elexpe Ane , Tolentino-Cortez Tarson , Gulak Marina , Bruzos-Cidón Cristina , Torrecilla María , Astigarraga Egoitz , Barreda-Gómez Gabriel
Published: March 2021

Acetylcholinesterase (AChE) is responsible for hydrolyzing the acetylcholine neurotransmitter, bringing an end point to cholinergic neurotransmission. Thus, AChE is the primary target of a wide spectrum of compounds used as pesticides, nerve agents or therapeutic drugs for neurodegenerative diseases such as Alzheimer’s disease (AD). This enzyme is heterogeneously distributed in the brain showing different activity depending on the nervous region. Therefore, the aim of this work is to report a novel technology that enables the simultaneous determination of tissue specific AChE activity, as well as the analysis and screening of specific inhibitors, by using cell membrane microarrays. These microarrays were composed of cell membranes, isolated from 41 tissues, organs and brain areas, that were immobilized over a slide, maintaining the functionality of membrane proteins. To validate this platform, demonstrating its usefulness in drug discovery as a high throughput screening tool, a colorimetric protocol to detect the membrane-bound AChE activity was optimized. Thus, rat cortical and striatal AChE activities were estimated in presence of increased concentrations of AChE inhibitors, and the donepezil effect was assessed simultaneously in 41 tissues and organs, demonstrating the major potential of this microarray’s technology.
Improved Trapping and Handling of an Arboreal, Montane Mammal: Red Panda Ailurus fulgens
Authors: Bista Damber , Lama Sonam Tashi , Weerman Janno , Sherpa Ang Phuri , Pandey Purushotam , Thapa Madhuri Karki , Acharya Haribhadra , Hudson Nicholas J. , Baxter Greg S. , Murray Peter John
Published: March 2021

It is sometimes essential to have an animal in the hand to study some of their ecological and biological characteristics. However, capturing a solitary, cryptic, elusive arboreal species such as the red panda in the wild is challenging. We developed and successfully tested a protocol for tracking, trapping, immobilization, and handling of red pandas in the wild in eastern Nepal. We established a red panda sighting rate of 0.89 panda/day with a capture success rate of 0.6. We trapped and collared one animal in 3.7 days. On average, we took nearly 136 (range 50–317) min to capture an animal after spotting it. Further processing was completed in 38.5 (21–70) min. Before capture, we found it difficult to recognize the sex of the red panda and to differentiate sub-adults above six months from adults. However, body weight, body length, tail length, shoulder height, and chest girth can be used for diagnosis, as these attributes are smaller in sub-adults. Our method is a welfare-friendly way of trapping and handling wild red pandas. We report new morphometric data that could serve as a guide for field identification.
Effect of Leukoreduction on Hematobiochemical Parameters and Storage Hemolysis in Canine Whole Blood Units
Authors: Antognoni Maria Teresa , Marenzoni Maria Luisa , Misia Ambra Lisa , Avellini Luca , Chiaradia Elisabetta , Gavazza Alessandra , Miglio Arianna
Published: March 2021

Storage lesions (SLs) occur when the red blood cell quality is altered during the preservation of blood units. Pre-storage leukoreduction would limit the number of SLs. The aims of this study were to evaluate the effectiveness of a leukoreduction filter for human use and the effect of pre-storage leukoreduction on some ematobiochemical parameters in stored canine whole blood. Seven canine blood units were tested. Each one was divided into two units—one leukoreduced (LRWB) and one non-leukoreduced (nLRWB). On each unit, we determined the complete blood count (CBC), lactate-dehydrogenase (LDH), electrolytes (Na+, K+, Cl−), morphological index (MI) and hemolysis, on storage days 0, 7, 14, 21, 28, 35, and 42. Leukoreduction allowed a 98.30% recovery of the RBC count, retaining 99.69% and 94.91% of WBCs and PLTs, respectively. We detected a significant increase of LDH and MI with strongly higher values in nLRWB compared to LRWB. A progressive increase in electrolytes and LDH concentrations was observed as indices of stored hemolysis. LDH showed significantly lower values in LRWB units compared to nLRWB, suggesting its release from leukocytes. In the majority of units, hemolysis reached 1% on the 42nd day of storage. We assert the human leukoreduction filter effectiveness on canine whole blood, and we recommend using nLRWB before day 14, especially for critically ill patients. The difference of the basal hemolysis (day 0) percentages observed between subjects suggests that more studies should be performed to confirm a possible inter-individual donor biological variability of RBC membrane resistance, as happens in humans.
Occurrence of Multidrug Resistant Escherichia coli in Raw Meat and Cloaca Swabs in Poultry Processed in Slaughter Slabs in Dar es Salaam, Tanzania
Authors: Mgaya Fauster X. , Matee Mecky I. , Muhairwa Amandus P. , Hoza Abubakar S.
Published: March 2021

This cross-sectional study was conducted between January and June 2020, in five large poultry slaughter slabs in Dar es Salaam, Tanzania. Purposive sampling was used to select broilers and spent layers, from which meat and cloaca swabs were collected to determine the occurrence of multidrug resistant (MDR) Escherichia coli. Identification of isolates was done using API 20E, and antimicrobial susceptibility testing was performed as per CLSI (2018) guidelines. EBSL (CTX-M, TEM, SHV) and plasmid mediated quinolone (qnrA, qnrB, qnrS and aac(6′)-Ib-cr) were screened using PCR. Out of 384 samples, 212 (55.2%) were positive for E. coli, of which 147 (69.3%) were resistant to multiple drugs (MDR). Highest resistance was detected to tetracycline (91.9%), followed by sulfamethoxazole-trimethoprim (80.5%), ampicillin (70.9%), ciprofloxacin (40.2%) and 25% cefotaxime, gentamycin (10.8%) and imipenem (8.6%) (95% CI, p < 0.01). Out of the E. coli-positive samples, ten (10/212) (4.7%) were ESBL producing E. coli, of which CTX-M was detected in two isolates and quinolones resistant gene (qnrS) in eight, while TEM, SHV, qnrA, qnrB and aac(6′)-lb-cr were not detected. The high level of resistance and multidrug resistance imply these antibiotics are ineffective, add unnecessary cost to poultry farmers and certainly facilitate emergence and spread of resistance.
Clonal Lineages, Antimicrobial Resistance, and PVL Carriage of Staphylococcus aureus Associated to Skin and Soft-Tissue Infections from Ambulatory Patients in Portugal
Authors: Ferreira Carolina , Costa Sofia Santos , Serrano Maria , Oliveira Ketlyn , Trigueiro Graça , Pomba Constança , Couto Isabel
Published: March 2021

Staphylococcus aureus (S. aureus) is a leading cause of skin and soft-tissue infections (SSTIs) in the community. In this study, we characterized a collection of 34 S. aureus from SSTIs in ambulatory patients in Portugal and analyzed the presence of Panton–Valentine leucocidin (PVL)-encoding genes and antibiotic-resistance profile, which was correlated with genetic determinants, plasmid carriage, and clonal lineage. Nearly half of the isolates (15, 44.1%) were methicillin-resistant Staphylococcus aureus (MRSA) and/or multidrug resistant (MDR). We also detected resistance to penicillin (33/34, 97.1%), fluoroquinolones (17/34, 50.0%), macrolides and lincosamides (15/34, 44.1%), aminoglycosides (6/34, 17.6%), and fusidic acid (2/34, 5.9%), associated with several combinations of resistance determinants (blaZ, erm(A), erm(C), msr(A), mph(C), aacA-aphD, aadD, aph(3′)-IIIa, fusC), or mutations in target genes (fusA, grlA/gyrA). The collection presented a high genetic diversity (Simpson’s index of 0.92) with prevalence of clonal lineages CC5, CC22, and CC8, which included the MRSA and also most MDR isolates (CC5 and CC22). PVL-encoding genes were found in seven isolates (20.6%), three methicillin-susceptible Staphylococcus aureus (MSSA) (ST152-agrI and ST30-agrIII), and four MRSA (ST8-agrI). Plasmid profiling revealed seventeen distinct plasmid profiles. This work highlights the high frequency of antimicrobial resistance and PVL carriage in SSTIs-related S. aureus outside of the hospital environment.
How Bacterial Redox Sensors Transmit Redox Signals via Structural Changes
Authors: Lee In-Gyun , Lee Bong-Jin
Published: March 2021

Bacteria, like humans, face diverse kinds of stress during life. Oxidative stress, which is produced by cellular metabolism and environmental factors, can significantly damage cellular macromolecules, ultimately negatively affecting the normal growth of the cell. Therefore, bacteria have evolved a number of protective strategies to defend themselves and respond to imposed stress by changing the expression pattern of genes whose products are required to convert harmful oxidants into harmless products. Structural biology combined with biochemical studies has revealed the mechanisms by which various bacterial redox sensor proteins recognize the cellular redox state and transform chemical information into structural signals to regulate downstream signaling pathways.
Impact of Polyphenolic-Food on Longevity: An Elixir of Life. An Overview
Authors: Meccariello Rosaria , D’Angelo Stefania
Published: March 2021

Aging and, particularly, the onset of age-related diseases are associated with tissue dysfunction and macromolecular damage, some of which can be attributed to accumulation of oxidative damage. Recently, growing interest has emerged on the beneficial effects of plant-based diets for the prevention of chronic diseases including obesity, diabetes, and cardiovascular disease. Several studies collectively suggests that the intake of polyphenols and their major food sources may exert beneficial effects on improving insulin resistance and related diabetes risk factors, such as inflammation and oxidative stress. They are the most abundant antioxidants in the diet, and their intake has been associated with a reduced aging in humans. Polyphenolic intake has been shown to be effective at ameliorating several age-related phenotypes, including oxidative stress, inflammation, impaired proteostasis, and cellular senescence, both in vitro and in vivo. In this paper, effects of these phytochemicals (either pure forms or polyphenolic-food) are reviewed and summarized according to affected cellular signaling pathways. Finally, the effectiveness of the anti-aging preventive action of nutritional interventions based on diets rich in polyphenolic food, such as the diets of the Blue zones, are discussed.
A Traceable and Authenticated IoTs Trigger Event of Private Security Record Based on Blockchain
Authors: Chen Chin-Ling , Lim Zi-Yi , Liao Hsien-Chou , Deng Yong-Yuan
Published: March 2021

Recently, private security services have become increasingly needed by the public. The proposed scheme involves blockchain technology with a smart contract. When a private security company signs a contract with a client, they install an Internet of Things (IoTs) device in the client’s house and connect it with the IoT main controller; then, the IoT main controller connects to the security control center (SCC). Once there is an event triggered (e.g., a break-in or fire incident) by the IoTs device, the controller sends a message to the SCC. The SCC allocates a security guard (SG) to the incident scene immediately. After the task is accomplished, the SG sends a message to the SCC. All of these record the messages and events chained in the blockchain center. The proposed scheme makes security event records have the following characteristics: authenticated, traceable, and integral. The proposed scheme is proved by a security analysis with mutual authentication, traceability, integrity, and non-repudiation. The known attacks (e.g., man-in-the-middle attack, replay attack, forgery attack) are avoided by message encryption and a signing mechanism. Threat models in the communication phase can also be avoided. Finally, computation cost, communication performance, and comparison with related works are also discussed to prove its applicability. We also provide an arbitration mechanism, so that the proposed scheme can reduce disputes between private security companies and the client.
Evaluation of Algae-Based Biodiesel Production Topologies via Inherent Safety Index (ISI)
Authors: González-Delgado Angel Darío , García-Martínez Janet B. , Barajas-Solano Andrés F.
Published: March 2021

Increasing energy needs have led to soaring fossil fuel consumption, which has caused several environmental problems. These environmental aspects along with the energy demand have motivated the search for new energy systems. In this context, biofuels such as biodiesel have been developing into a substitute for conventional fuels. Microalgae are considered a promising option for biodiesel production due to their high lipid content. Therefore, it is important to analyze the technical aspects of the biodiesel production system. In this work, the inherent safety analysis of three emerging topologies for biodiesel production from microalgae was performed using the inherent safety index (ISI) methodology. Selected topologies include biodiesel production via lipid extraction and transesterification, in-situ transesterification, and hydrothermal liquefaction (HTL). The results revealed that the processes are inherently unsafe achieving total inherent safety index scores of 30, 29, and 36. The main risks in the cases were associated with the chemical safety index. Operating conditions represented no risk for topologies 1 and 2, while for topology 3 pressure and temperature were identified as critical variables. In general, topology 2 showed better performance from a safety perspective.
Comparison of Endurance Time Prediction of Biceps Brachii Using Logarithmic Parameters of a Surface Electromyogram during Low-Moderate Level Isotonic Contractions
Authors: Cho Chang-ok , Jeong Jin-Hyoung , Kim Yun-jeong , Jang Jee Hun , Lee Sang-Sik , Lee Ki-young
Published: March 2021

At relatively low effort level tasks, surface electromyogram (sEMG) spectral parameters have demonstrated an inconsistent ability to monitor localized muscle fatigue and predict endurance capacity. The main purpose of this study was to assess the potential of the endurance time (Tend) prediction using logarithmic parameters compared to raw data. Ten healthy subjects performed five sets of voluntary isotonic contractions until their exhaustion at 20% of their maximum voluntary contraction (MVC) level. We extracted five sEMG spectral parameters namely the power in the low frequency band (LFB), the mean power frequency (MPF), the high-to-low ratio between two frequency bands (H/L-FB), the Dimitrov spectral index (DSI), and the high-to-low ratio between two spectral moments (H/L-SM), and then converted them to logarithms. Changes in these ten parameters were monitored using area ratio and linear regressive slope as statistical predictors and estimating from onset at every 10% of Tend. Significant correlations (r > 0.5) were found between log(Tend) and the linear regressive slopes in the logarithmic H/L-SM at every 10% of Tend. In conclusion, logarithmic parameters can be used to describe changes in the fatigue content of sEMG and can be employed as a better predictor of Tend in comparison to the raw parameters.
In Vitro Cytotoxic Evaluation and Apoptotic Effects of Datura innoxia Grown in Saudi Arabia and Phytochemical Analysis
Authors: Al-Zharani Mohammed , Nasr Fahd A. , Alqahtani Ali S. , Cordero Mary Anne W. , Alotaibi Amal A. , Bepari Asmatanzeem , Alarifi Saud , Daoud Ali , Barnawi Ibrahim O. , Daradka Haytham M.
Published: March 2021

Datura innoxia is an important species of Solanaceae family with several purposes in folk medicine. This study intends to explore the cytotoxic effect of D. innoxia on various cancer cell proliferation. D. innoxia ethanolic extract’s effect on the progression of the cell cycle and the induction of apoptosis were investigated by flow cytometry. Further, real-time PCR was employed to confirm apoptosis initiation. In addition, active phytochemicals of D. innoxia was identified by gas chromatography–mass spectroscopy (GC-MS). The cell viability study revealed that the ethanolic extract of D. innoxia demonstrated potent cytotoxicity, with an IC50 value of 10 μg/mL against LoVo colon cancer cells. Cell cycle staining with propidium iodide revealed that D. innoxia treatment leads to cell accumulation in the sub-G1 phase. Using the Annexin V-FITC/PI assay, the ethanolic extract was found to cause a dose-dependent increase in early and late apoptosis when compared to control cells. Apoptosis as the mode of cell death was also confirmed by the increased expression of p53, bax and caspase-8, -9, and -3 along with downregulation of Bcl-2. GC-MS analysis displayed that 3,5-Dihydroxybenzoic acid (16.53%), heneicosyl formate (14.14%), 2,3-dimethyl-3-pentanol (12.89%), 2-hydroxy-4-methyl pentanoic acid (5.19%) were the main phytoconstituents. These findings conclude that D. innoxia causes cell death through apoptosis, suggesting more attention should be paid to further exploration of the active components from D. innoxia responsible for the observed activities.
Phonetic Variation Modeling and a Language Model Adaptation for Korean English Code-Switching Speech Recognition
Authors: Lee Damheo , Kim Donghyun , Yun Seung , Kim Sanghun
Published: March 2021

In this paper, we propose a new method for code-switching (CS) automatic speech recognition (ASR) in Korean. First, the phonetic variations in English pronunciation spoken by Korean speakers should be considered. Thus, we tried to find a unified pronunciation model based on phonetic knowledge and deep learning. Second, we extracted the CS sentences semantically similar to the target domain and then applied the language model (LM) adaptation to solve the biased modeling toward Korean due to the imbalanced training data. In this experiment, training data were AI Hub (1033 h) in Korean and Librispeech (960 h) in English. As a result, when compared to the baseline, the proposed method improved the error reduction rate (ERR) by up to 11.6% with phonetic variant modeling and by 17.3% when semantically similar sentences were applied to the LM adaptation. If we considered only English words, the word correction rate improved up to 24.2% compared to that of the baseline. The proposed method seems to be very effective in CS speech recognition.
Comparative Tissue Responses of Marine Mollusks on Seasonal Changes in the Northern Adriatic Sea
Authors: Topić Popović Natalija , Krbavčić Martina , Barišić Josip , Beer Ljubić Blanka , Strunjak-Perović Ivančica , Babić Sanja , Lorencin Vanesa , Matulić Daniel , Tomljanović Tea , Čož-Rakovac Rozelindra
Published: March 2021

In the shallow Northern Adriatic, marine mollusks are affected by bottom trawling and seafood disturbance. Seasonal oscillations of oceanographic factors additionally influence their physiology, stress responses and survival. Tissue responses to seasonal variations in green ormer (Haliotis tuberculata L.) and Mediterranean scallop (Pecten jacobaeus L.) in the Northern Adriatic have not been reported. Hence, their biochemical and antioxidant defense properties over seasons were studied and the microanatomical structure of their tissue was correlated with function. Histological analysis of gonads revealed two peaks of gonadal maturation and spawning during the spring/summer period and winter season for scallops, and one peak during the fall for ormers. The gonadal maturation of both species was correlated with their seasonal variations of metabolic demands and antioxidant capacity. The lipid vacuoles of tubuloacinar terminations in the digestive gland differed between the two species; in scallop they are several-fold larger in size and number. Low temperatures in winter contributed to a decline in enzymatic antioxidant defense in scallop tissues, having lower superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity, and higher concentrations of thiobarbituric acid reactive substances (TBARS) and total antioxidant status (TAS). In ormers, winter induced lower TAS, TBARS, SOD and GPx concentrations. The significant difference of winter TAS and TBARS levels between ormers and scallops was correlated with variations in their reproductive cycles, as well as in antioxidant defense systems. The most important factor for stress-related parameters for both species in this work was found to be the season-induced temperature change.
Applicability of Clinical Decision Support in Management among Patients Undergoing Cardiac Surgery in Intensive Care Unit: A Systematic Review
Authors: Pereira Miguel , Concheiro-Moscoso Patricia , López-Álvarez Alexo , Baños Gerardo , Pazos Alejandro , Pereira Javier
Published: March 2021

The advances achieved in recent decades regarding cardiac surgery have led to a new risk that goes beyond surgeons’ dexterity; postoperative hours are crucial for cardiac surgery patients and are usually spent in intensive care units (ICUs), where the patients need to be continuously monitored to adjust their treatment. Clinical decision support systems (CDSSs) have been developed to take this real-time information and provide clinical suggestions to physicians in order to reduce medical errors and to improve patient recovery. In this review, an initial total of 499 papers were considered after identification using PubMed, Web of Science, and CINAHL. Twenty-two studies were included after filtering, which included the deletion of duplications and the exclusion of titles or abstracts that were not of real interest. A review of these papers concluded the applicability and advances that CDSSs offer for both doctors and patients. Better prognosis and recovery rates are achieved by using this technology, which has also received high acceptance among most physicians. However, despite the evidence that well-designed CDSSs are effective, they still need to be refined to offer the best assistance possible, which may still take time, despite the promising models that have already been applied in real ICUs.
Pulmonary Effects Due to Physical Exercise in Polluted Air: Evidence from Studies Conducted on Healthy Humans
Authors: Araneda Oscar F. , Kosche-Cárcamo Franz , Verdugo-Marchese Humberto , Tuesta Marcelo
Published: March 2021

Physical inactivity has caused serious effects on the health of the population, having an impact on the quality of life and the cost of healthcare for many countries. This has motivated government and private institutions to promote regular physical activity, which, paradoxically, can involve health risks when it is carried out in areas with poor air quality. This review collects information from studies conducted on healthy humans related to the pulmonary effects caused by the practice of physical activity when there is poor air quality. In addition, several challenges related to the technological and educational areas, as well as to applied and basic research, have been identified to facilitate the rational practice of exercise in poor air quality conditions.
Assimilation of Dynamic Combined Finite Discrete Element Methods Using the Ensemble Kalman Filter
Authors: Godinez Humberto C. , Rougier Esteban
Published: March 2021

Simulation of fracture initiation, propagation, and arrest is a problem of interest for many applications in the scientific community. There are a number of numerical methods used for this purpose, and among the most widely accepted is the combined finite-discrete element method (FDEM). To model fracture with FDEM, material behavior is described by specifying a combination of elastic properties, strengths (in the normal and tangential directions), and energy dissipated in failure modes I and II, which are modeled by incorporating a parameterized softening curve defining a post-peak stress-displacement relationship unique to each material. In this work, we implement a data assimilation method to estimate key model parameter values with the objective of improving the calibration processes for FDEM fracture simulations. Specifically, we implement the ensemble Kalman filter assimilation method to the Hybrid Optimization Software Suite (HOSS), a FDEM-based code which was developed for the simulation of fracture and fragmentation behavior. We present a set of assimilation experiments to match the numerical results obtained for a Split Hopkinson Pressure Bar (SHPB) model with experimental observations for granite. We achieved this by calibrating a subset of model parameters. The results show a steady convergence of the assimilated parameter values towards observed time/stress curves from the SHPB observations. In particular, both tensile and shear strengths seem to be converging faster than the other parameters considered.
Comparison of DFN Modelled Microfracture Systems with Petrophysical Data in Excavation Damaged Zone
Authors: Kiuru Risto , Király Dorka , Dabi Gergely , Jacobsson Lars
Published: March 2021

Physical and petrographic properties of drill core specimens were determined as a part of investigations into excavation damage in the dedicated study area in the ONKALO® research facility in Olkiluoto, Western Finland. Microfractures in 16 specimens from two drillholes were analysed and used as a basis for fractal geometry-based discrete fracture network (DFN) modelling. It was concluded that the difference in resistivity between pegmatoid granite (PGR) and veined gneiss (VGN) specimens of similar porosity was likely due to differences in the types of microfractures. This hypothesis was confirmed from microfracture analysis and simulation: fractures in gneiss were short and mostly in one preferred orientation, whereas the fractures in granite were longer and had two preferred orientations. This may be due to microstructure differences of the rock types or could suggests that gneiss and granite may suffer different types of excavation damage. No dependencies on depth from the excavated surface were observed in the geometric parameters of the microfractures. This suggests that the excavation damaged zone cannot be identified based on the changes in the parameters of the microfracture networks, and that the disturbed layer observed by geophysical methods may be caused by macro-scale fractures.
Compressive Strength Testing of Hybrid Concrete-Filled Fiber-Reinforced Plastic Tubes Confined by Filament Winding
Authors: Kang In-Kyu , Kim Sun-Hee
Published: March 2021

In this study, an experiment on compressive strength of the hybrid concrete-filled fiber-reinforced polymer (FRP) tube (CFFT) confined by filament winding was conducted to improve the longitudinal strength while considering the thickness of filament winding as a variable. A maximum error of 17% was observed when the results of performing the finite element analysis (FEA) by applying the mechanical properties of the fiber-reinforced polymer (FRP) materials suggested in previous studies were compared to those of the compressive strength experiment on the hybrid-CFFT. Moreover, a maximum error of 15% was exhibited when the results derived from the strength equation proposed by analyzing the compressive strength experiment were compared. Furthermore, the compressive strength of the hybrid-CFFT increased by up to 14% when the longitudinal compressive strength of the pre-tensioned spun high strength concrete (PHC) pile and concrete-filled tube (CFT) were compared.
A Rule-Based System for Human Performance Evaluation: A Case Study
Authors: Mikulić Iva , Lisjak Dragutin , Štefanić Nedeljko
Published: March 2021

The issues of many organizations are related to the proper evaluation of human performance and efficient decision-making. The expert system application within the decision-making process is not a novelty, but the widespread of its implementation regarding performance evaluation has not been recognized yet. To overcome this problem, a case study of rule-based expert system application in the decision-making process regarding human performance in periodical technical inspection stations in Croatia is presented. The rule-based expert system improves the quality of traditional decision-making as designed rules provide a visual, transparent, and accurate comparison of observed values with the expected values. Moreover, it provides easy problem identification. Therefore, rules regarding periodical technical inspection inspectors’ performance are designed and embedded in the expert system architecture. However, more effort should be made into data analysis to define parameters and their relations for the purpose of designing rules. Thus, the binary logistic regression and an ANOVA statistical test were conducted to identify which parameters can be assumed as relevant indicators regarding the performance of periodical technical inspection inspectors. In this study, the expert system application has resulted in faster response, greater efficiency, and increased objectivity. That is of utmost importance for providing an efficient and transparent periodical technical inspection system.
Study of Quinoline Insoluble (QI) Removal for Needle Coke-Grade Coal Tar Pitch by Extraction with Fractionalized Aliphatic Solvents and Coke Formation Thereof
Authors: An Jung-Chul , Lee Seong-Young , Park Joo-Il , Ha Manyoul , Shim Joongpyo , Hong Ikpyo
Published: March 2021

Various fractionalized solvents with different paraffinicities were adopted to maximize the efficiency of the quinoline insoluble (QI) extraction process for coal tar pitch. In addition, highly pressurized conditions combined with raised temperature (4 bar at 300 °C) were used to accelerate the reaction kinetics of the extraction process. The QI content of purified coal tar pitch was analyzed to be 0.1% at a process yield of up to 72% as a solvent with a K-factor of 10 and above was used. Purified coal tar pitch was then processed to form anisotropic coke using a lab-scale tube bombe reactor. The texture observed under a polarized light microscope showed an anisotropic flow domain, a unique morphological feature of needle coke. The additives and reaction conditions used in this study for QI extraction for coal tar pitch were found to be effective and feasible as preliminary processing in needle coke production.
Spectrum Awareness for Cognitive Radios Supported by Radio Environment Maps: Zonal Approach
Authors: Kaniewski Paweł , Romanik Janusz , Golan Edward , Zubel Krzysztof
Published: March 2021

In this paper, we present the concept of the Radio Environment Map (REM) designed to ensure electromagnetic situational awareness of cognitive radio networks. The map construction techniques based on spatial statistics are presented. The results of field tests done for Ultra High Frequency (UHF) range with different numbers of sensors are shown. Exemplary maps with selected interpolation techniques are presented. Control points where the signal from licensed users is correctly estimated are identified. Finally, the map quality is assessed, and the most promising interpolation techniques are selected.
Various Generative Adversarial Networks Model for Synthetic Prohibitory Sign Image Generation
Authors: Dewi Christine , Chen Rung-Ching , Liu Yan-Ting , Yu Hui
Published: March 2021

A synthetic image is a critical issue for computer vision. Traffic sign images synthesized from standard models are commonly used to build computer recognition algorithms for acquiring more knowledge on various and low-cost research issues. Convolutional Neural Network (CNN) achieves excellent detection and recognition of traffic signs with sufficient annotated training data. The consistency of the entire vision system is dependent on neural networks. However, locating traffic sign datasets from most countries in the world is complicated. This work uses various generative adversarial networks (GAN) models to construct intricate images, such as Least Squares Generative Adversarial Networks (LSGAN), Deep Convolutional Generative Adversarial Networks (DCGAN), and Wasserstein Generative Adversarial Networks (WGAN). This paper also discusses, in particular, the quality of the images produced by various GANs with different parameters. For processing, we use a picture with a specific number and scale. The Structural Similarity Index (SSIM) and Mean Squared Error (MSE) will be used to measure image consistency. Between the generated image and the corresponding real image, the SSIM values will be compared. As a result, the images display a strong similarity to the real image when using more training images. LSGAN outperformed other GAN models in the experiment with maximum SSIM values achieved using 200 images as inputs, 2000 epochs, and size 32 × 32.
June–July Temperature Reconstruction of Kashmir Valley from Tree Rings of Himalayan Pindrow Fir
Authors: Malik Rayees , Sukumar Raman
Published: March 2021

The Himalaya is one of the major mountain ecosystems that is most likely to be impacted by climate change. The main drawback in understanding climate change in the remote Himalayan ecosystems is the lack of long-term instrumental climate records. Reconstructing past climates from tree-rings offers a useful proxy for adding data to the instrumental climate records. In this study, climatically sensitive tree-rings of Himalayan fir (Abies pindrow) were used for reconstruction of mean June–July temperatures of Kashmir valley. Total ring-width chronology was built from 60 tree-ring cores growing near the higher altitudinal limits of the species. The radial growth showed a strong positive response to growing season temperature. The strong response of site chronology to mean June–July temperatures was used for reconstruction purposes. Mean June–July temperatures of Kashmir valley were reconstructed since 1773 from residual site chronology. Though the reconstruction did not show any strong long-term trend, on a centennial-scale, 20th-century summers were the warmest with a mean annual summer temperature of 22.99 °C. Seven of the warmest years and five of the warmest decades were seen in the 20th century. The reconstruction for 1773–2012 showed 23 extreme hot summers above the hot threshold of a 23.47 °C mean temperature and 19 extreme cold years below the cold threshold of a 22.46 °C mean summer temperature. The cold years in the reconstruction did not coincide with known volcanic eruptions. This reconstruction will help in providing a better understanding of regional climate change.