bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Partial Defoliation on Vine Carbohydrate Concentration and Flavonoid Production in Cranberries
Authors: Onayemi Olanike O. , Neto Catherine C. , Vanden Heuvel Justine E.
Published: February 2019

The effect of partial defoliation (rate and timing) on vine carbohydrate concentration and the development of phenolic compounds in field-grown `Stevens' cranberry fruit was investigated in two experiments. In Expt. 1, partial defoliation rates of 0%, 18%, 39%, and 53% of total leaf area were applied before new growth, at fruit set, at midfruit development, and preharvest. In Expt. 2, treatments of 0% and 34% removal of new leaves were applied at postfruit set, and at midfruit development. In both experiments, upright samples were harvested for carbohydrate analysis 10d after defoliation, and fruit were removed for analysis before commercial harvest of the site. While total berry phenolic concentration was unaffected by partial defoliation in both studies, the separate pools of flavonoid compounds were affected differently by treatment. In Expt. 1, total flavonol concentration at harvest was improved by the highest rate of partial defoliation (53% of total leaf area) at both fruit set and midfruit development. Total anthocyanin concentration was improved by partial defoliation rates of 39% and 53% of total leaf area compared to the 18% defoliation treatment, but was not affected by timing of defoliation. Pearson correlation coefficients indicated that total flavonol concentration was positively correlated with vine total nonstructural carbohydrate concentration at preharvest, while total anthocyanin concentration was negatively correlated with vine soluble carbohydrates, starch, and total nonstructural carbohydrate concentration at midfruit development. In Expt. 2, total phenolics, flavonols, and anthocyanins were unaffected by partial defoliation; however there was a negative correlation between total anthocyanin concentration in the fruit and soluble carbohydrate concentration in the vine at midfruit development. In these experiments, partial defoliation early in the growing season improved total flavonols and total anthocyanins. Production of flavonols and anthocyanins appeared to be regulated independently of each other.
Effects of Boric Acid Concentration and Shading on Growth, Leaf Physiology, and Anatomy of Guzmania
Authors: Kuo C.Y. , Yeh D.M.
Published: February 2019

Guzmania lingulata (L.) Mez. `Cherry' were grown in coco chips and fertigated with half-strength Hoagland solution containing various concentrations of boric acid. Excessive boron induced changes in growth, relative chlorophyll content, and leaf anatomy were investigated. Plants treated with 5 mg·L–1 or higher boric acid concentration had reduced SPAD-502 readings and Fv/Fm values and increased leaf necrosis in the lower leaves. Boron was distributed unevenly within a leaf, with the maximum concentration in the leaf tip. Increased necrotic length and new leaves with necrosis were evident where average whole leaf boron concentration was higher than 170 μg·g–1 on dry weight basis. More leaf growth and higher transpiration or stomatal conductance were recorded in plants under 40% (average 676 μmol·m–2·s–1 PPF at noon) than 76% (average 270 μmol·m–2·s–1 PPF at noon) shade. Excessive boron was not found to affect epidermal cells or water storage tissue, but caused browning and shriveling of the chlorenchyma cells.
Effect of Plant Growth Regulators on Propagule Formation in Hemerocallis spp. and Hosta spp.
Authors: Leclerc Mélanie , Caldwell Claude D. , Lada Rajasekaran R. , Norrie Jeffrey
Published: February 2019

Field experiments were conducted in 2002 and 2003 to evaluate the effects of selected plant growth regulators on propagule production in Hemerocallis `Happy Returns' and Hosta `Gold Standard'. Benzyladenine (BA), chlormequat chloride (Cycocel), ethephon (Ethrel), prohexadione calcium (Apogee), and an experimental preparation of commercial seaweed extract (Acadian Seaplants Limited Liquid Seaweed Concentrate) amended with BA and IBA were tested at two times of application and three rates of application. Results with Hemerocallis showed that the application of the seaweed/PGR mixture at 3000 mg·L–1, Cycocel at 3000 mg·L–1 or BA at 2500 mg·L–1 applied at flowering, increased the number of plants producing two divisions compared to control plants. In Hosta, no increase in divisions under any treatments was observed.
Mandarin Attributes Preferred by Consumers in Grocery Stores
Authors: Campbell Benjamin L. , Nelson Robert G. , Ebel Robert C. , Dozier William A.
Published: February 2019

For most grocery stores, external quality standards require that premium mandarins be orange, unblemished, and large. Thus, for consumers to differentiate among the premium mandarins on any dimension other than price, additional positioning attributes must be evaluated. This study considered consumer preferences for price ($2.18/kg, $4.39/kg, or $15.41/kg), packaging (1.36 kg of loose fruit, 1.36-kg bag, 2.27-kg box, or 0.23-kg clamshell with peeled fruit sections), type of mandarin (clementine, satsuma, tangerine), shelf life from the day of purchase (3, 14, or 31 days), and vitamin C content (with or without a label stating high in vitamin C). A conjoint survey was conducted in four grocery stores located in Birmingham and Montgomery, Ala. In total, 289 respondents used a 7-point intention-to-buy scale to rate photographs of 16 product profiles. Six market segments were identified, based on maximal similarity of preferences within each segment and maximal differences between segments. A simulation was conducted of the effect that an introduction of peeled-and-sectioned satsumas would have on the market share and gross revenue of other mandarins. This product showed great potential, but should be offered in a product mix that includes the loose form as well. Labeling for vitamin C was preferred by all segments, but did not contribute much to the intention-to-buy rating. Awareness and recognition of satsumas needs to be addressed in promotional campaigns. The longest shelf life was the first choice of almost half the respondents.
Examining the Vascular Pathway of Sweet Cherries Grafted onto Dwarfing Rootstocks
Authors: Olmstead Mercy A. , Lang N. Suzanne , Lang Gregory A. , Ewers Frank W. , Owens Shirley A.
Published: February 2019

Dye transport through vascular pathways was examined in tissues surrounding the graft union of second-leaf, field-grown trees of `Lapins'/Gisela 5 (`Gi 5') (dwarfing) and `Lapins'/'Colt' (nondwarfing). Excavated, intact trees were allowed to take up xylemmobile dye via transpiration for 6 h before sectioning the tree into scion, graft union, and rootstock tissue. `Lapins'/'Gi 5' had a significantly larger stem cross-sectional area in the central graft union than did `Lapins'/'Colt'. Per unit cross section, dye transport of both `Lapins'/'Gi 5' and `Lapins'/'Colt' was significantly less in the graft union than in rootstock sections, with still less transported to scion tissues in `Lapins'/'Gi 5'. `Lapins'/'Gi 5' had a tendency to produce vascular elements oriented obliquely to the longitudinal axis of the tree. Dye was distributed more uniformly axially and radially across the graft union in `Lapins'/'Colt' than in `Lapins'/'Gi 5', with an apparent accumulation of dye in `Lapins'/'Gi 5' graft union. Xylem vessel diameters and vessel hydraulic diameters (VDh) were smaller overall in `Lapins'/'Gi 5' than in `Lapins'/'Colt'; however, graft unions in both had smaller VDh than did rootstock sections. These observations suggest reduced transport efficiency of xylem vessels in the graft union in `Lapins'/'Gi 5' may be due to smaller vessels, vascular abnormalities and/or increased amounts of callus and parenchyma tissue.
Compatibility of Three Miticides with the Predatory Mites Neoseiulus californicus McGregor and Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae)
Authors: Cloyd Raymond A. , Galle Cindy L. , Keith Stephen R.
Published: February 2019

In this study, we report on the compatibility of two commercially available predatory mites, Neoseiulus californicus and Phytoseiulus persimilis, with three miticides used in greenhouse production systems to control the twospotted spider mite, Tetranychus urticae. We determined the lethal effects of the miticides chlorfenapyr, spiromesifen, and bifenazate to both predatory mite species 24 hours after exposure to spray applications in petri dishes. Two rates of chlorfenapyr (0.40 and 0.81 mL/2-L) and spiromesifen (0.15 and 0.31 mL·L–1), and one rate of bifenazate (0.62 mL·L–1) were used. All rates were based on the manufacturer label recommendations for twospotted spider mite. Both rates of chlorfenapyr and spiromesifen, and the single rate of bifenazate were not harmful to N. californicus with percent live mite values ≥85% for chlorfenapyr and ≥95% for spiromesifen, and 93% for bifenazate. However, these same miticides were substantially toxic to P. persimilis with percent live mite values of ≤63% for all the miticides tested. Based on the results of this study, the miticides chlorfenapyr, spiromesifen, and bifenazate are compatible with N. californicus whereas these miticides are toxic to P. persimilis indicating a difference in susceptibility based on predatory mite species.
Effect of Thermotherapy on Elimination of Apple Stem Grooving Virus and Apple Chlorotic Leaf Spot Virus for In vitro-cultured Pear Shoot Tips
Authors: Wang Liping , Wang Guoping , Hong Ni , Tang Rongrong , Deng Xiaoyun , Zhang Hong
Published: February 2019

Apple stem grooving virus (ASGV) and apple chlorotic leaf spot virus (ACLSV) are two major viruses of pear. In this study, in vitro thermotherapy was carried out at 37°C for 25, 30 and 35 days followed by subculturing of meristem tips of different sizes to eliminate ASGV and ACLSV from pear plants. Virus titers in heat-treated shoot tips were evaluated by ELISA testing of regenerated plants. Results showed that thermotherapy for 35 days significantly decreased the titer of ASGV and ACLSV in cultures regenerated from tips of main and axillary shoots, especially in those from explants 1 mm in length from the tip of meristems. Dot-blot hybridization of biotinylated cDNA probes derived from ACLSV and ASGV was used to detect these viruses in crude tissue extracts of in vitro-grown pear plants. Intense signals were consistently detected in untreated plant samples equivalent to less than 0.5 mg tissue. Comparison of signals from dot-blot hybridization and ELISA absorbance values (A405) confirmed that dot-blot hybridization had a higher sensitivity than PAS-ELISA. Dot-blot hybridization could detect viruses with a titer below the threshold level of ELISA. These results indicate that dot-blot hybridization is a useful tool for large-scale surveys of viruses, which facilitates the production of virus-free propagation materials in certification and sanitation programs. Results of PAS-ELISA and dot-blot hybridization showed that high virus elimination efficiency was achieved by a combination of thermotherapy for 35 days and in vitro culture of 1 mm meristem tips.
NaOH Scarification and Stratification Improve Germination of Iris lactea var. chinensis Seed
Authors: Sun Y.C. , Zhang Y.J. , Wang K. , Qiu X.J.
Published: February 2019

Iris lactea seed is characterized mainly by physiological dormancy. Two experiments were conducted to investigate the effect of NaOH treatment and stratification on Iris seed germination. In Experiment 1, seeds were treated with 14.38 m NaOH for 0 to 28 hours. In Experiment 2, NaOH treated and nontreated seeds were stratified under 7 °C and moistened condition for 0 to 40 days. As results, NaOH treatment for 20 hours effectively removed seedcoat and improved germination percentage from 0% to 56% compared to control (0 hours). However, germination percentage started to decrease after 20 hours. Stratification for 40 days further improved germination percentage of NaOH treated seeds to >80%, but did not affected seeds without NaOH treatment. Results demonstrate that combination of NaOH treatment and stratification is an effective practice to break Iris seed dormancy and improve germination percentage.
Influence of Fertilization Practices on Live Oak Wound Closure
Authors: Ali A. D. , Luley Christopher J.
Published: February 2019

A 2-year study was conducted in southwest Florida to evaluate the effects of quick vs. slow-release nitrogen sources commonly used in landscapes on wound closure in live oak [Quercus virginiana (Mill.)]. Fertilized trees displayed nonsignificant shoot growth and leaf color when compared to nonfertilized ones. Neither fertilizer type, nor rate of application appeared to affect wound closure rates over the two year measurement period. This study showed increased replication will be needed when studying landscape trees, since trends in known primary growth response to fertilization were present but were not statistically significant. Fertilization effects on secondary growth including diameter increase and wound closure rate, were correspondingly not significant. Secondary growth responses may be difficult to demonstrate in live oak when using landscape trees especially at lower nitrogen rates currently being recommended in the industry.
Effects of Trinexapac-Ethyl on the Salinity Tolerance of Two Ultradwarf Bermudagrass Cultivars
Authors: Baldwin Christian M. , Liu Haibo , McCarty Lambert B. , Bauerle William L. , Toler Joe E.
Published: February 2019

Studies on bermudagrasses (Cynodon spp.) have demonstrated variability in salinity response among species and cultivars. However, information on ultradwarf bermudagrass cultivars in relative salinity tolerance associated with trinexapac-ethyl (TE) [4-(cyclopropyl-α-hydroxy-methylene)-3,5-dioxocyclohexanecarboxylic acid ethyl ester], a cyclohexanedione type II plant growth regulator (PGR), remains unknown. Therefore, two replicated greenhouse studies were conducted to determine the salinity tolerance of two ultradwarf bermudagrass cultivars treated with TE on turfgrass quality (TQ), total root biomass, and root and shoot tissue nutrient concentration. Turfgrasses included `TifEagle' and `Champion' bermudagrass (Cynodondactylon(L.) Pers. × C. transvaalensisBurtt-Davy). Daily sodium chloride (NaCl) exposure was 0, 12.90 (8,000 ppm), 25.80 (16,000 ppm), and 38.71 dS·m–1 (24,000 ppm). Biweekly TE applications (active ingredient 0.02 kg·ha–1) were initiated 2 weeks after salinity exposure. `Champion' was more salt-tolerant than `TifEagle' based on TQ and root mass. At 12.90, 25.80, and 38.71 dS·m–1 of NaCl, nontreated (without TE) `Champion' consistently outperformed nontreated `TifEagle' with greater TQ on most rating dates. At 12.90 dS·m–1, TE treated `Champion' (8.0) had greater TQ than nontreated `TifEagle' (6.1) at week 10. Regardless of TE application, after 2 weeks of applying 25.80 dS·m–1 of NaCl, both cultivars fell below acceptable TQ (<7). When averaged across all salinity treatments, applying TE four times at 0.02 kg·a.i./ha in two week intervals enhanced root growth for both bermudagrass cultivars by 25%. Also, both cultivars decreased root mass as salinity levels increased. Non TE-treated `TifEagle' had 56% and 40% less root and shoot Na uptake compared to TE treated cultivars at 25.80 dS·m–1. In conclusion, the two bermudagrass cultivars responded differently when exposed to moderate levels of NaCl.
Inhibition of `Zenith' Zoysiagrass Seedling Emergence and Growth by Perennial Ryegrass Leaves and Roots
Authors: Zuk Alan J. , Fry Jack D.
Published: February 2019

Establishment of seeded `Zenith' zoysiagrass (Zoysia japonica Steud.) in an existing sward of perennial ryegrass (Lolium perenne L.) is difficult, and chemicals arising from perennial ryegrass leaf and root tissue may contribute to establishment failure. Experiments were done to evaluate zoysiagrass emergence and growth in soil amended with perennial ryegrass leaves or roots, or after irrigation with water in which perennial ryegrass leaves or roots had previously been soaked. Compared to unamended soil, soil amended with perennial ryegrass leaves at 12% and 23% by weight reduced zoysiagrass seedling number 20% and 26%, respectively; root area and mass were reduced 50% when amendments comprised 12% of soil weight. Similar reductions in zoysiagrass seedling emergence and growth were observed in a second soil amendment study, regardless of whether perennial ryegrass was treated with glyphosate or not. Soil mixed with perennial ryegrass leaves, but not roots, at 12% by weight had a high soil conductivity (5.1 dS·m–1), which could have contributed to reduced zoysiagrass emergence and growth. More than 50% fewer zoysiagrass seedlings emerged and root mass was up to 65% lower when irrigated with water in which perennial ryegrass leaves or roots at 5, 10, or 20 g·L–1 were previously soaked for 48 hours. Zoysiagrass leaf area, and root length and area, were also lower when irrigated with water previously containing perennial ryegrass roots. Perennial ryegrass leaves and roots have the capacity to inhibit emergence and growth of `Zenith' zoysiagrass seedlings, which could negatively affect stand establishment.
A Phylogeny of Pelargonium Based on TRAP Markers
Authors: Palumbo Rose E. , Hong Wai-Foong , Hu Jinguo , Krause Charles , Locke James , Craig Richard , Tay David , Wang Guo-Liang
Published: February 2019

Pelargonium is one of the priority genera collected by the Ornamental Plant Germplasm Center (OPGC). In order to protect future breeders from a loss of genetic diversity, the OPGC collects heirloom cultivars, breeding lines, and wild species. The current Pelargonium collection consists primarily of cultivars originating from P. ×hortorum and P. ×domesticum. Our project was designed to analyze the current collection in order to facilitate the maintenance of a more-diverse core collection. We have expanded our TRAP (Target Region Amplified Polymorphism) analysis from 120 plants with one primer set to include 780 plants with four primer sets. Each primer set consists of a labeled arbitrary primer paired with a gene-specific primer, and two different fluorescent labels were used to allow multiplexed PCR reactions. We scored about 90 markers in each of the first two primer sets and about 60 markers in each of the second two. In comparisons between the phylogeny and the morphology and taxonomy of these plants, we show some matching clusters that may be explained by the breeding history of the plants.
Molecular Evidence for an Asian Origin and a Unique Westward Migration of Species in the Genus Castanea via Europe to North America
Authors: Lang Ping , Dane Fenny , Kubisiak Thomas L. , Huang Hongwen
Published: February 2019

The genus Castanea (Fagaceae), which contains three sections and seven species, is widely distributed in the deciduous forests of the Northern Hemisphere. The phylogeny of Castanea was estimated using DNA sequence data from five different regions of the chloroplast genome. Sequencing results support the genus Castanea as a paraphyletic group with C. crenata, the Japanese chestnut, representing an early divergence in the genus. The three Chinese species form a strongly supported sister clade to the North American and European clade. A unique westward expansion of extant Castanea species is hypothesized with Castanea originating in eastern Asia, an initial diversification within Asia during the Eocene, followed by intercontinental dispersion and divergence between the Chinese and European/North American species during the Oligocene and a split between the European and North American species in the early Miocene. The differentiation within North America and China might have occurred in late Miocene or early Pliocence. The North America species are supported as a clade with C. pumila var. ozarkensis, the Ozark chinkapin, as the basal lineage, sister to the group comprising C. pumila var. pumila, the Allegheny chinkapin, and C. dentata, the American chestnut. Morphological evolution of one nut per bur in the genus may have occurred independently on two continents.
Effects of 5-chloro-3-methyl-4-nitro-1H-pyrazole and Ethephon on Citrus Leaf Function and Water Relations
Authors: Li Kuo-Tan , Burns Jackie , Pozo Luis , Syvertsen Jim
Published: February 2019

To determine the effects of abscission compounds 5-chloro-3-methyl-4-nitro-1H-pyrazole (CMNP) and ethephon on citrus leaf function and water relations, we applied CMNP at 0, 200, 500, 1000, or 2000 ppm, or ethephon at 400 or 800 ppm, to canopies of fruiting potted and field citrus trees during the harvest season. Both compounds induced fruit and leaf drop after 3 days of application, especially at high concentrations. Low concentrations of CMNP (0, 200, or 500 ppm) or either ethephon treatments did not affect leaf photosystem II efficiency, as indicated by leaf chlorophyll fluorescence (Fv/Fm). High concentrations of CMNP (1000 or 2000 ppm) immediately reduced photosystem II efficiency in leaves and fruit peel. However, Fv/Fm of leaves remaining on the trees was gradually restored and close to the level of control after 4 days of treatment. Both compounds had little effect on chlorophyll content, ratio of chlorophyll a to chlorophyll b, leaf water content, and mid-day leaf water potential. The results suggest that CMNP at recommended concentrations (200 to 500 ppm) effectively reduced fruit attachment force with little herbicidal effect on leaves.
Irrigation Requirements and Drought Response of Two Ornamental Grass Species
Authors: Alvarez Erin , Scheiber Sloane M. , Sandrock David R.
Published: February 2019

Water use is the most important environmental issue facing the horticulture industry. As a result, many water management districts are recommending native plants for their putative low-water requirements. Numerous textbooks and trade journals claim native plants use less water than non-natives; however, previous research found no difference in water use efficiency in the field between native and non-native species. Furthermore, recommendations of ornamental grasses for use as low-maintenance and low-water-requiring landscape plants have recently escalated. This study evaluated non-native Miscanthus sinensis `Adagio' and the native Eragrostis spectabilis for irrigation requirements and drought response in a landscape setting. To simulate maximum stress, both species were planted into field plots in an open-sided, clear polyethylene covered shelter. Each species was irrigated on alternating days at 0, 0.25, 0.5, or 0.75 L for a 90-day period. Growth index and height were recorded at biweekly intervals, and final shoot and root dry masses were taken at completion of the study. Significant treatment and species effects were found for height, growth index, shoot dry weight, and biomass. Plants receiving 0.75 L of irrigation had the greatest growth, and non-irrigated plants grew significantly less. Comparisons between species found growth was greatest among Eragrostis spectabilis plants for all parameters.
Yield, PFD Interception, and Crop Load Relationships in `Royal Gala' Apples
Authors: de Cortazar Victor Garcia , Reginato Gabino
Published: February 2019

Three different parameters were tested to estimate yield in `Royal Gala' apples. These are: a) parameters related to crop load—fruits per tree, fruits per cm2 of branch cross-sectional area, and fruits per hectare; b) parameters related with PFD interception: average fraction of PFD intercepted, total PFD intercepted during the season; and c) combination of the parameters a) and b). The data set was composed of measurements of PFD interception once a month and of yield components on various commercial apple orchards of the variety `Royal Gala' in the central zone of Chile between 2003 and 2006. The orchards were managed for high production, but there were differences of plantation distance, age, and size between them. Also, inside the orchard there were differences between trees. For the trees studied, there were variations of a factor of 10 for crop load, branch cross-sectional area, and tree size estimated as fractional interception of PFD at the beginning of the season. In spite of the big differences between trees, simple equations were fitted between yield and load parameters with coefficients of determination >0.95. Research funded by FONDECYT-Chile grant 1930695.
(4) 1-MCP Preharvest Spray Application to Synchronize Harvest and Improve Fruit Quality of Cantaloupe
Authors: Leskovar Daniel , Agehara Shinsuke , Goreta Smiljana
Published: February 2019

The objective of this study was to determine the effects of 1-MCP preharvest spray application on harvest synchrony, maturity, fruit quality, and marketable yield of cantaloupe. Seeds were planted in a commercial field on 16 Mar. (early planting, cv. Caravelle) and 4 Apr. (late planting, cv. Mission) 2005. Standard plant population, fertilization, irrigation, and pest control practices were followed. We evaluated three 1-MCP rates (5, 10, or 25 g·ha-1 a.i.) at three preharvest spraying times for the early (22, 15, and 7 days before harvest, DBH) or once for the late planting experiment (4 DBH). An additional test (late planting) compared fruit quality after storage for melons dipped with 1-MCP (0 or 10 mg·L-1). Fruits were harvested six times during June 2005 (early planting) and once on 19 July 2005 (late planting) and fruit quality parameters were measured at harvest and after storage. The preharvest 1-MCP application slightly delayed maturity and improved early harvest synchrony, but did not affect total marketable or yield by fruit size regardless of timing or rate of application. There was no effect of 1-MCP rate or application timing on fruit quality at harvest or after cold storage, except for an increased in fruit firmness (10%) in one of the six harvests. However, fruits treated with 1-MCP spray at 25 g·ha-1 a.i. (late planting) had higher firmness than those treated with lower rates after 9 days of storage. In addition, 1-MCP postharvest dipping significantly improved fruit firmness; however, a `greening' was evident in the fruit surface. Our results suggest that cantaloupe fruit quality was less affected by early preharvest spray application of 1-MCP applied at less than 25 g·ha-1 a.i. as compared to postharvest applications.
(6) Sucker Removal and Pruning Affect on Heirloom Tomato Yield and Fruit Size
Authors: Kline Wesley L. , Garrison Stephen A. , Sudal June F.
Published: February 2019

Heirloom tomato production is increasing in the Eastern United states as consumer demand increases. Pruning and suckering heirloom tomatoes have not been studied to see if there is any need for this labor-intensive activity. A 2-year study was undertaken to evaluate whether pruning or suckering would affect yield or fruit size for two heirloom cultivars (`Mortgage Lifter' and `Prudens Purple'). The treatments imposed on the cultivars were 1) removing all suckers from the second or third stem down after the flower cluster; 2) removing the bottom two suckers, or 3) removing no suckers. Pruning had no effect on early yield or fruit size (harvests 1–4). Mid-season (harvests 5–7) total and marketable yields were significantly higher for removing two suckers or not suckering over the other two treatments for year 1, but not year 2. The tomato fruit size was only reduced for the non-suckering treatment. There were no statistical differences among the pruning treatments for yield or fruit size for late season harvests (8-10) for both years. Marketable yields were statistically higher for no suckering over the two- and three-stem treatments, but not different from two suckers when all harvests were combined for the season for year 1. No statistical differences were observed in year 2. However, fruit size was reduced when not suckering compared to the other treatments. The cultivar `Prudens Purple' did have higher total and marketable yield than `Mortgage Lifter' for both early and total combined harvests, but not for mid- or late-season harvests in year 1. There were no statistical differences between the two cultivars for year 2.
(8) Supplemental Potassium Source and Processing Tomato Quality
Authors: Gunter Christopher , Francis David , McIntyre Alba Clivati
Published: February 2019

Yellow shoulder disorder (YSD) is a physiological disorder of processing tomato that affects both the appearance and nutritional quality of the fruit. This disorder reduces the suitability of fruit intended for the whole-peeled and diced product markets. The YSD involves an interaction between plant genotype and the environment. A number of soil factors have been related to the incidence of YSD, including organic matter, phosphorous, K/Mg ratios, and soil K. Varieties of tomatoes differ in their susceptibility to color disorders, thus variety selection offers growers one strategy to manage this color disorder. The use of supplemental K application at a time when plants are blooming and actively growing offers a second strategy for management of YSD. To this end, a field study was conducted at the Southwest Purdue Agricultural Program in southwestern Indiana to study the effects of different sources of K on the color and quality of tomato fruit. Potassium chloride, potassium nitrate, and potassium sulfate were applied at first flowering in a solid, broadcast application. Appropriate controls were used to balance the nutrients supplied in addition to K. Supplemental K, regardless of source, improved fruit hue, though the trend was not always statistically significant between treatments. Variety specific effects were observed. This is a complex disorder and its management will entail minimizing risk of incidence through careful selection of variety and field location.
(10) Effect of the Floating Cover on Cucumber (Cucumis sativus L.) Yield
Authors: Tene Marcelino Bazán , Larios Javier Farías , Aguirre José Gerardo López , Juárez Francisco Radillo , Sánchez Jorge Rogelio Flores
Published: February 2019

Production of horticultural crops is important throughout the world, with high consumption mainly in Europe and the United States of America. Cucumber is consumed both fresh and processed. This vegetable generates important profits for producing countries such as Mexico, which, in 2003, produced 435,897 tons of fresh fruit with a value of 1.190 million Mexican pesos (around USD $109 million). The objective of this work was to evaluate the effect of the floating cover on the yield of fresh cucumbers. Four treatments and five repetitions were imposed in a randomized distribution: covered 20 days (C20) post-emergence, covered until beginning of flowering (CF), covered until beginning of harvest (CIC), and without cover (SC). Data at the first and fourth harvest were analyzed. Results showed that, in the first harvest, treatment C20 days was highest in fruit yield (3.11 ton/ha); Treatment CIC had 2.47 ton/ha, and SC produced 1.66 ton/ha. Data obtained in the fourth harvest, treatment C20, had the highest yield, with 24.12 ton/ha, and treatment CF had a yield of 21.11 ton/ha, but there was not significant difference (Tukey's P < 0.05).
(179) Using Quantitative Real Time PCR to Predict Levels of Botrytis allii in Stored Onion
Authors: Coolong Timothy , Randle William , Walcott Ronald
Published: February 2019

Onion (Allium cepa L.) is an economically important vegetable in the United States. Though considered a minor crop in terms of total acreage, onions have high value when compared to other crops and, nationally, their value approaches $800 million. Because harvested onions are routinely stored for long periods, disease can be a major obstacle to the industry. The primary disease reported in stored onions is botrytis neck rot caused by the fungus Botrytis allii (syn. B. aclada). Losses from neck rot can approach 35% of the stored crop. In order to accurately quantify the level of B. allii inoculum in bulbs at harvest to be able to predict potential botrytis neck rot in storage, a quantitative real-time PCR test to quantify levels of B. allii DNA present in onion bulb tissue has been developed. We have employed the TaqMan real time PCR assay and report log-linear (R2= 0.9915) relationship between B. allii DNA concentration and cycle threshold (Ct) value with a detection limit of 5 pico gram/microliter DNA. In addition, a log-linear standard curve plotting mycelial dry weight against Ct value has been developed to allow prediction of mycelial weight in onion tissue at harvest. Currently, the ability of this test to predict botrytis neck rot during storage is being tested.
(182) Comparison of Aqueous and Gaseous Application of 1-Methylcyclopropene on Ripening of Tomato Fruit Harvested at Two Stages of Maturation
Authors: Jeong Jiwon , Lee James , Huber Donald J.
Published: February 2019

This study was performed to characterize the physiological responses of tomato (Lycopersicon esculentum L.) fruit harvested at either 10% to 30% or 30% to 60% color change and treated with two forms of 1-methylcyclopropene (1-MCP). Tomato fruit were treated either by submersion for 1 min in 1-MCP aqueous solution at the ambient temperature or by exposure for 12 h at 20 °C in air with 1-MCP gas, then stored at 20 °C. The concentrations (1.0, 5.0, or 10.0 μL·L-1) in 1-MCP aqueous solution were achieved through addition of 0.5, 2.5, or 5.0 g of AFxRD-300 powder (2.0% formulation, Agro-Fresh, Inc.) to 10 L of the de-ionized water, following manufacturer's instructions. 1-MCP (0.5 μL·L-1) gas in a 174-L container was achieved through addition of 0.22 g of SmartFresh® powder (0.14% formulation, Agro-Fresh, Inc.) to 100 mL of tap water. Both forms of 1-MCP significantly delayed ripening of fruit at the two initial ripeness stages, as noted by a significant delay in fruit softening and peel color change. The firmness of 30% to 60% color change tomatoes was significantly retained in response to gaseous or aqueous 1-MCP. Control fruit softened rapidly and reached the minimum marketable firmness value (about 5 N) within 8 days of storage at 20 °C, whereas fruit treated with gaseous 1-MCP (0.5 μL·L-1) or aqueous 1-MCP (1.0 or 5.0 μL·L-1) reached the same stage after 16, 20, or 24 days, respectively. Firmness retention was also highly significant for 10% to 30% color change tomatoes treated with both forms of 1-MCP. The highest concentration of aqueous 1-MCP (10.0 μL·L-1) did not result in a further delay in ripening compared with treatment at 5.0 or 1.0 μL·L-1 1-MCP.
(88) Sour Orange: A Source of Unique Bioactive Limonoid Glucosides
Authors: Vikram Amit , Jayaprakasha G.K. , Patil Bhimanagouda S.
Published: February 2019

Our recent studies have shown that certain citrus limonoids protect from colon cancer based on cell and animal studies. Animal studies also suggest that citrus juice protects from osteoporosis. To understand the structure–function relationship through animal studies requires a large amount of purified limonoids. Since certain limonoids are present in low concentration, it is a challenge to obtain the required quantity of different limonoids. In this context, we report the purification of limonin 17-ß-D glucopyranosides (LG), and deacetylnomilinic acid 17-ß-D glucopyranoside (DNAG). However, DNAG was isolated in relatively large amount from sour orange (Citrus aurantium L.) seeds. Defatted seed powder was extracted with methanol and purified using column chromatography to obtain multigrams of DNAG. While LG was found in lower concentration, a large concentration of hesperidin was also purified in this process. This project is based upon work supported by the USDA-CSREES under Agreement USDA IFAFS #2001 52102 02294 and USDA #2005-34402-14401 “Designing Foods for Health” through the Vegetable & Fruit Improvement Center.
(229) Fertility Affects Cutting Production and Quality of Rooted Cuttings of Iva imbricata
Authors: Raymer Josiah , Thetford Mack , Miller Debbie
Published: February 2019

Seacost Marshelder (Iva imbricata Walter [Asteraceae]), a dominant Atlantic and Gulf region seashore plant, is a broad-leaved plant with a potential for building and stabilizing foredunes in the South Atlantic coast of the United States, and is recognized as an important food for beach mice. Two experiments were conducted where nursery liners were potted as stock plants and produced at four rates of fertility using Osmocote Plus (15N:9P2O5: 12K2O; 8–9 m formulation) applied as a top dress at 5.5, 11.0, 15.0, and 21.0 g/3.7-L container. The experiment was arranged as a CRD with 12 single-plant replicates of each fertility rate. Stock plant growth, cutting production, and subsequent rooting characteristics (percent rooting, root number, length) were evaluated for cuttings harvested at each of four harvests (30-day interval). Stock plant height increased as fertility rate increased for all harvests. After the first harvest, plant height did not differ among fertility rates above 5.5 g. Growth indices demonstrated that a 21.0-g application of fertilizer was necessary to increase stock plant size. The number of cuttings produced per stock plant increased linearly with increasing rate of fertility for all harvests. Cutting weight of individual cuttings increased linearly with an increase in fertilizer rate for harvests one and two, but cutting weight did not differ thereafter. The rooting response differed depending on the time of harvest. Percent rooting decreased with an increase in fertility rate for harvests two and three. Increased fertility rate did result in a decrease in root number for harvest one, but no further decrease was evident thereafter. Root length did not differ among harvest dates or fertility rates.
(38) Planting Depth and Tree Performance
Authors: Jarecki Mark W. , Williams David J. , Kling Gary J.
Published: February 2019

Growers, nurseries, landscape contractors and installers, and those responsible for maintenance have observed a trend that trees are too deep within the root ball. This study addresses the relationship between planting depth and its effect on tree survival, root growth, root architecture, and caliper growth. The experiment was initiated to determine the effect of planting depth on nursery-grown trees. Three-year-old, 2.1–2.7 m, bare-root liners of Acer platanoides `Emerald Lustre', Fraxinus americana `Autumn Purple', Fraxinus pennsylvanica `Patmore', and Gleditsia triacanthos f. inermis `Shade Master' were planted in April 2004 in a completely randomized design with 20 replications per treatment per species. The trees were selected so that the distance between the graft union and the trunk flare was consistent. Trees were planted with the graft union 15.2 cm below the soil surface, or with the base of the graft union at the finished grade or with the trunk flare at the finished grade. The trees were grown in a nursery field setting with minimal supplemental watering. There were no differences in stem caliper growth at the end of two seasons in any of the four species. Root dry mass, stem elongation, and rooting structure were determined on a representative sample of trees while others were planted into the landscape for a long-term study of the effects of the original planting depth on landscape performance.
(102) The Dynamic Model and Rest-breaking Agents in California `French' Prune Production
Authors: Glozer Kitren , Niederholzer Franz J.
Published: February 2019

Use of rest-breaking chemicals may partially substitute for chill requirement in “French' prune. Many California prune growers use oil in the dormant season to tighten and advance bloom, with application timing judged by experience and calendar date. Other rest-breaking agents have become commonly used in California cherry production and their application is generally timed by chill portion accumulation, calculated by the Dynamic Model. We evaluated the effects of treatments of dormant oil or CAN17 (calcium ammonium nitrate) + Entry on budbreak and bloom progression in `French' prune with applications timed at regular intervals. While most treatments improved fruit set and reduced reproductive bud death, an optimum range for both types of rest-breaking treatments was found for advancement and compression of bloom. All rest-breaking treatments advanced fruit maturity equally, compared to the untreated control, as measured by fruit firmness. Although chill hour (hours ≤7°C) calculations might also be used for timing these treatments, when chill portion and chill hour accumulations are compared for the 2004–05 dormant season at several different sites, differences from site-to-site are small for chill portions, and much greater for chill hours. This fact supports experimental evidence from numerous California trials in sweet cherry in which rest-breaking treatment timings based on the Dynamic Model tend to be more consistent than the timings based on the “chill hour” model.
(163) Organic and Biorational Fungicides for Managing Gray Mold on Raspberries
Authors: Sanders Graham , Sanchez Elsa , Demchak Kathleen
Published: February 2019

The increased demand for organic and sustainably grown produce has resulted in a demand for information on organic and biorational fungicides. The efficacy of these fungicides is often not established, yet they are aggressively advertised. In 2005 the efficacy of six organic and biorational fungicides and two controls were evaluated on field-grown red raspberries (Rubus idaeus `Prelude' and `Nova') for gray mold (Botrytis cinerea) management. Phytotoxicity of the fungicide treatments was evaluated on a weekly basis following each fungicide application. Fruit was harvested by hand, sorted into marketable and unmarketable categories and weighed. Subsamples of fruit were evaluated for postharvest disease development. Data analysis showed `Nova' was more susceptible to phytotoxicity than `Prelude'. The application of Phostrol resulted in the highest phytotoxicity rating when compared to all other fungicide treatments. The water spray control, standard fungicide (Captan/Elevate rotation) control, Endorse, and Lime Sulfur treatments resulted in negligible phytotoxity ratings. Applying Milstop, Milstop + Oxidate, and Oxidate + Vigor Cal Phos resulted in similar intermediate phytotoxicity ratings. Differences in marketable yield were nonexistent for the two cultivars and eight fungicide treatments. The predominant diseases observed in the postharvest evaluations were gray mold, blue mold (Penicillium sp.), and rhizopus soft rot (Rhizopus sp.) and/or mucor mold (Mucor sp.). This evaluation will be repeated in 2006.
(164) The Influence of Mulch Applications on Vegetation Dynamics in Organically Managed Highbush Blueberry
Authors: Burkhard Nicole E. , Lynch Derek H. , Percival David C.
Published: February 2019

Within-row weed management of highbush blueberry (Vaccinium corymbosum L.) is reliant upon herbicide applications. However, in organic production, herbicides are typically not permitted and alternative methods must be used. The impact of thick (25-cm) mulch applications on weed pressure in an organic production system was initiated at a commercial operation in Nova Scotia, Canada, during 2005. A split-plot experimental design was used with five blocks (replications), six treatments, and five plants per split plot (cv. Duke). The whole-plot factor consisted of mulch/fertility treatments and included: i) control (no amendment); ii) ammonium sulphate fertilizer (30 kg·ha-1 N); iii) pelletized poultry manure (60 kg·ha-1 N); iv) pine needles (80 t·ha-1); v) horse manure and sawdust compost (550 t·ha-1); and vi) seafood waste compost (360 t·ha-1). The split-plot factor consisted of level of hand weeding (–/+). Weed control was assessed by sampling percent ground cover and weed shoot biomass in three 0.25-m2 quadrats in nonweeded subplots. Blueberry leaf N content, plant canopy volume, and berry yield (fresh weight and number) were recorded. The manure/sawdust compost and pine needle treatments had the lowest weed biomass and percent ground cover values, thereby providing the best weed control. Weed shoot biomass, blueberry leaf N, plant canopy volume, and berry yield were greatest in the seafood waste compost treatment. Results from this preliminary study indicate the potential of using these groundcover treatments to improve organic cultural management practices.
(165) University of Guam Triton Farm
Authors: Marutani Mari , Brown John , Acosta Mark , Sablan Joseph , Afaisen Sheeka , McConnell James
Published: February 2019

A grant to construct a “Model Farm” on Guam and the Virgin Islands was approved in 2000 by the USDA/CSREES/Initiative for Future Agriculture and Food Systems Program. The main goal was to establish an integrated model farm that had potential benefits for small agricultural enterprises operated by Asian-Pacific and Caribbean Islanders. University of Guam Triton Farm was established on 3.75 acres (1.5 ha) of the Agricultural Experiment Station. Initially we conducted a soil survey, and established windbreaks/hedgerows. We also built the foundation for aquaculture/aquaponic system, field irrigation systems, and animal production facilities. Then, we planted long-term fruit and ornamental plants while growing short-term vegetable crops for quick returns. Currently we raise tilapia (Oreochromis niloticus), goats (Capra hercus) and layer-chickens (Gallus gallus). We grow banana (Musa spp.), calamansi (X Citrofortunello mitis, hot peppers (Capsicum spp.), cucumbers (Cucumis sativus), lettuce (Lactuca sativa), eggplants (Solanum melongena), and Ti-leaf (Cordyline terminalis). We also try to develop value-added products using local produce. Occasionally we investigate other potential commodities and operational schemes for the farm. These must be suitable for Guam's agro-climate and social and economic structure. We focus on conservation of natural materials, composting, and sustainable agriculture. Education and outreach activity is also an important component of the farm to disseminate technologies and to educate young children about farming.
(276) Assessing Genetic Diversity of Spinach Germplasm using TRAP Markers
Authors: Hu Jinguo , Mou Beiquan , Vick Brady A.
Published: February 2019

Target region amplified polymorphism (TRAP) markers were used to evaluate genetic variability among 48 accessions of spinach (Spinacia oleracea L.), an economically important leafy vegetable crop in many countries. Thirty-eight accessions collected and preserved by the USDA National Plant Germplasm System (NPGS) and 10 commercial hybrids were used in the current study. For assessing genetic diversity within accessions, DNA samples were prepared from nine to 12 individual seedlings from six germplasm accessions and two hybrids. Relatively high levels of polymorphism was found within accessions based on 61 polymorphic TRAP markers generated with two fixed primers derived from the Arabidopsis-type telomere repeat sequence and two arbitrary primers. For evaluating inter-accession variability, DNA was extracted from a bulk of six to 10 seedlings of each accession. Of the 1092 fragments amplified by 14 primer combinations, 96 (8.8%) were polymorphic and discriminated the 48 accessions from each other. The average pair-wise genetic similarity coefficient (Dice, Nei) was 57.5% with a range from 23.2 to 85.3%. A dendrogram was constructed based on the similarity matrix. It was found that the genetic relationships were not highly correlated with the geographic locations in which the accessions were collected. However, seven commercial hybrids were grouped in three separate clusters, suggesting that the phenotype-based breeding activities have effect on the genetic variability. This study demonstrated that TRAP markers are effective for fingerprinting and evaluating genetic variability of spinach germplasm.