bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Analysis of Tetra(2-naphthyl)tetracyano-porphyrazine and Its Iron Complex as Photosensitizers for Anticancer Photodynamic Therapy
Authors: Shestakova Lydia N. , Lyubova Tatyana S. , Lermontova Svetlana A. , Belotelov Artem O. , Peskova Nina N. , Klapshina Larisa G. , Balalaeva Irina V. , Shilyagina Natalia Y.
Published: November 2022

Photodynamic therapy (PDT) is a rapidly developing modality of primary and adjuvant anticancer treatment. The main trends today are the search for new effective photodynamic agents and the creation of targeted delivery systems with the function of controlling the release of the agent in the tumor. Recently, the new group of cyanoarylporphyrazine dyes was reported, which combine the properties of photosensitizers and sensors of the local microenvironment. Such unique characteristics allow the release of the photosensitizer from the transport carrier to be assessed in real time in vivo. The aim of the present work was to compare the photophysical and photobiological properties of tetra(2-naphthyl)tetracyanoporphyrazine and its newly synthesized Fe(II) complex. We have shown that the chelation of the Fe(II) cation with the porphyrazine macrocycle leads to a decrease in molar extinction and an increase in the quantum yield of fluorescence and photostability. We demonstrate that the iron cation significantly affects the rate of dye accumulation in cells, the dark toxicity and photodynamic activity, and the direction of the changes depends on the particular cell line. However, in all the cases, the photodynamic index of a metal complex was higher than that of a metal-free base. In general, both of the compounds were found to be very promising for PDT, including for the use with transport delivery systems, and can be recommended for further in vivo studies.
Stable Cavitation-Mediated Delivery of miR-126 to Endothelial Cells
Authors: He Stephanie , Singh Davindra , Yusefi Hossein , Helfield Brandon
Published: November 2022

In endothelial cells, microRNA-126 (miR-126) promotes angiogenesis, and modulating the intracellular levels of this gene could suggest a method to treat cardiovascular diseases such as ischemia. Novel ultrasound-stimulated microbubbles offer a means to deliver therapeutic payloads to target cells and sites of disease. The purpose of this study was to investigate the feasibility of gene delivery by stimulating miR-126-decorated microbubbles using gentle acoustic conditions (stable cavitation). A cationic DSTAP microbubble was formulated and characterized to carry 6 µg of a miR-126 payload per 109 microbubbles. Human umbilical vein endothelial cells (HUVECs) were treated at 20–40% duty cycle with miR-126-conjugated microbubbles in a custom ultrasound setup coupled with a passive cavitation detection system. Transfection efficiency was assessed by RT-qPCR, Western blotting, and endothelial tube formation assay, while HUVEC viability was monitored by MTT assay. With increasing duty cycle, the trend observed was an increase in intracellular miR-126 levels, up to a 2.3-fold increase, as well as a decrease in SPRED1 (by 33%) and PIK3R2 (by 46%) expression, two salient miR-126 targets. Under these ultrasound parameters, HUVECs maintained >95% viability after 96 h. The present work describes the delivery of a proangiogenic miR-126 using an ultrasound-responsive cationic microbubble with potential to stimulate therapeutic angiogenesis while minimizing endothelial damage.
Plant-Community Vulnerability in Highly Fragmented Landscapes Is Higher in Secondary Forests Than in Old Growth Forests in the Andean–Amazonian Transition
Authors: Rodríguez-León Carlos H. , Roa-Fuentes Lilia L. , Sterling Armando , Suárez Juan Carlos
Published: November 2022

Increasing biodiversity in highly diverse plant communities can jointly increase ecosystem function and ecosystem vulnerability. This paradox requires further attention. This study analyzed the functional response of plant communities to above- and below-ground parameters along the chronosequence (degraded pastures (DP), early forests (EF), intermediate forests (IF), and old-growth forests (OF)) in two highly fragmented landscapes of the Colombian Amazon as an estimate of the level of functional vulnerability. Three sets of functional attributes were evaluated: (i) functional composition based on the community-weighted mean (CWM) of five traits; (ii) functional diversity based on the multi-trait indices and functional dispersion (FDis) of each individual trait; and (iii) the functional vulnerability at the community-level and species-level. The individual traits did not show a clear pattern along the chronosequence. However, the trend indicated an increase in the values of resource conservation traits with the age of abandonment. The functional response of the community did not vary between landscapes. Between DP and OF, there was a significant increase in functional diversity and a decrease in functional redundancy, which increased community-level vulnerability. Consequently, the more vulnerable species were observed in the IF and OF plots. In addition, a decrease in environmental parameters, such as penetration resistance, bulk density and Ca content, and an increase in slope, precipitation, electric conductivity, pH, clay, organic material, and P and N contents increased the vulnerability. We elucidated the need for secondary forest management in terms of conservation and restoration to maintain the capacity to respond to changing environmental conditions in highly fragmented landscapes in the Andean–Amazonian transition.
Development of Two-Dimensional Model of Photosynthesis in Plant Leaves and Analysis of Induction of Spatial Heterogeneity of CO2 Assimilation Rate under Action of Excess Light and Drought
Authors: Sukhova Ekaterina , Ratnitsyna Daria , Gromova Ekaterina , Sukhov Vladimir
Published: November 2022

Photosynthesis is a key process in plants that can be strongly affected by the actions of environmental stressors. The stressor-induced photosynthetic responses are based on numerous and interacted processes that can restrict their experimental investigation. The development of mathematical models of photosynthetic processes is an important way of investigating these responses. Our work was devoted to the development of a two-dimensional model of photosynthesis in plant leaves that was based on the Farquhar–von Caemmerer–Berry model of CO2 assimilation and descriptions of other processes including the stomatal and transmembrane CO2 fluxes, lateral CO2 and HCO3− fluxes, transmembrane and lateral transport of H+ and K+, interaction of these ions with buffers in the apoplast and cytoplasm, light-dependent regulation of H+-ATPase in the plasma membrane, etc. Verification of the model showed that the simulated light dependences of the CO2 assimilation rate were similar to the experimental ones and dependences of the CO2 assimilation rate of an average leaf CO2 conductance were also similar to the experimental dependences. An analysis of the model showed that a spatial heterogeneity of the CO2 assimilation rate on a leaf surface should be stimulated under an increase in light intensity and a decrease in the stomatal CO2 conductance or quantity of the open stomata; this prediction was supported by the experimental verification. Results of the work can be the basis of the development of new methods of the remote sensing of the influence of abiotic stressors (at least, excess light and drought) on plants.
Anticholinesterase Activity of Budmunchiamine Alkaloids Revealed by Comparative Chemical Profiling of Two Albizia spp., Molecular Docking and Dynamic Studies
Authors: Hussein Mai E. , Mohamed Osama G. , El-Fishawy Ahlam M. , El-Askary Hesham I. , Hamed Ahmed A. , Abdel-Aziz Marwa M. , Alnajjar Radwan , Belal Amany , Naglah Ahmed M. , Almehizia Abdulrahman A. , Al-Karmalawy Ahmed A. , Tripathi Ashootosh , El Senousy Amira S.
Published: November 2022

Alzheimer’s disease remains a global health challenge and an unmet need requiring innovative approaches to discover new drugs. The current study aimed to investigate the inhibitory activity of Albizia lucidior and Albizia procera leaves against acetylcholinesterase enzyme in vitro and explore their chemical compositions. Metabolic profiling of the bioactive plant, A. lucidior, via UHPLC/MS/MS-based Molecular Networking highlighted the richness of its ethanolic extract with budmunchiamine alkaloids, fourteen budmunchiamine alkaloids as well as four new putative ones were tentatively identified for the first time in A. lucidior. Pursuing these alkaloids in the fractions of A. lucidior extract via molecular networking revealed that alkaloids were mainly concentrated in the ethyl acetate fraction. In agreement, the alkaloid-rich fraction showed the most promising anticholinesterase activity (IC50 5.26 µg/mL) versus the ethanolic extract and ethyl acetate fraction of A. lucidior (IC50 24.89 and 6.90 µg/mL, respectively), compared to donepezil (IC50 3.90 µg/mL). Furthermore, deep in silico studies of tentatively identified alkaloids of A. lucidior were performed. Notably, normethyl budmunchiamine K revealed superior stability and receptor binding affinity compared to the two used references: donepezil and the co-crystallized inhibitor (MF2 700). This was concluded based on molecular docking, molecular dynamics simulations and molecular mechanics generalized born/solvent accessibility (MM–GBSA) calculations.
The Nitrogen Dynamics of Newly Developed Lignite-Based Controlled-Release Fertilisers in the Soil-Plant Cycle
Authors: Abhiram Gunaratnam , Grafton Miles , Jeyakumar Paramsothy , Bishop Peter , Davies Clive E. , McCurdy Murray
Published: November 2022

The effect of newly developed controlled-release fertilisers (CRFs); Epox5 and Ver-1 and two levels of Fe2+ applications (478 and 239 kg-FeSO4 ha−1) on controlling nitrogen (N) losses, were tested on ryegrass, in a climate-controlled lysimeter system. The Epox5 and Ver-1 effectively decreased the total N losses by 37 and 47%, respectively, compared to urea. Nitrous oxide (N2O) emissions by Ver-1 were comparable to urea. However, Epox5 showed significantly higher (p < 0.05) N2O emissions (0.5 kg-N ha−1), compared to other treatments, possibly due to the lock-off nitrogen in Epox5. The application of Fe2+ did not show a significant effect in controlling the N leaching loss and N2O emission. Therefore, a dissimilatory nitrate reduction and chemodenitrification pathways were not pronounced in this study. The total dry matter yield, N accumulation, N use efficiency and soil residual N were not significantly different among any N treatments. Nevertheless, the N accumulation of CRFs was lower in the first month, possibly due to the slow release of urea. The total root biomass was significantly (p < 0.05) lower for Epox5 (35%), compared to urea. The hierarchical clustering of all treatments revealed that Ver-1 outperformed other treatments, followed by Epox5. Further studies are merited to identify the potential of Fe2+ as a controlling agent for N losses.
Heat and Cold-Stressed Individuals of Pistacia lentiscus (Mastic Tree) Do Modify Their Secreting Profile
Authors: Stefi Aikaterina L. , Papaioannou Varvara , Nikou Theodora , Halabalaki Maria , Vassilacopoulou Dido , Christodoulakis Nikolaos S.
Published: November 2022

Seedlings from the germinated seeds of Pistacia lentiscus were cultured in plant growth chambers for three months. Then, the plants were separated into three groups. Each group was cultured under different conditions. The first group was left to grow under normal Mediterranean conditions, as those recorded in spring. The other group was subjected to a ten-day heat stress while the last one also suffered a cold stress for ten days. The anatomical features of the leaves (leaf thickness, epidermal cell thickness, number of palisade layers, and development) between these three groups differed. The stressed plants accumulated large amounts of phenolics within their mesophyll cells. The biomass of the cold-stressed plants was minor, while it was high for the control plants. The oxidative stress was hardly detectable in the leaves of the control plants, while their heat-stressed counterparts suffered the highest concentration of reactive oxygen species. Differences concerning the absorption spectra of the three groups of leaves were not significant. An interesting incompatibility between the three groups concerned the expression of L-Dopa Decarboxylase, which climbed significantly in the heat-stressed plants. Finally, an interesting variation was observed concerning the concentrations of some biogenic amines/amino acids. This variation can be correlated to the other stress-induced reactions of the plants and, in some cases, was impressive. In conclusion, environmental stress can shift Pistacia lentiscus’ metabolism to synthesize different biogenic products, which can be considered as exploitable for the pharmaceutical or food industry.
Fruit Biology of Coconut (Cocos nucifera L.)
Authors: Beveridge Fernanda Caro , Kalaipandian Sundaravelpandian , Yang Chongxi , Adkins Steve W.
Published: November 2022

Coconut (Cocos nucifera L.) is an important perennial crop adapted to a wide range of habitats. Although global coconut demand has increased sharply over the past few years, its production has been decreasing due to palm senility, as well as abiotic and biotic stresses. In fact, replanting efforts are impeded due to the lack of good quality seedlings. In vitro technologies have a great potential; however, their applications may take time to reach a commercial level. Therefore, traditional seed propagation is still critical to help meet the rising demand and its practice needs to be improved. To achieve an improved propagation via seeds, it is important to understand coconut fruit biology and its related issues. This review aims to provide a comprehensive summary of the existing knowledge on coconut fruit morpho-anatomy, germination biology, seed dispersal, distribution, fruit longevity and storage. This will help to identify gaps where future research efforts should be directed to improve traditional seed propagation.
Plant Responses to Biotic and Abiotic Stresses: Crosstalk between Biochemistry and Ecophysiology
Authors: Hussain Muhammad Iftikhar , Muscolo Adele , Ahmed Mukhtar
Published: November 2022

Biotic and abiotic stresses, such as drought, salinity, extreme temperatures (cold and heat) and oxidative stress, are often interrelated; these conditions singularly or in combination induce cellular damage [...]
MIR390 Is Involved in Regulating Anthracnose Resistance in Apple
Authors: Shi Jiajun , Jiang Qiu , Zhang Shuyuan , Dai Xinyu , Wang Feng , Ma Yue
Published: November 2022

As an important cash crop in China, apple has a good flavor and is rich in nutrients. Fungal attacks have become a major obstacle in apple cultivation. Colletotrichum gloeosporioides is one of the most devastating fungal pathogens in apple. Thus, discovering resistance genes in response to C. gloeosporioides may aid in designing safer control strategies and facilitate the development of apple resistance breeding. A previous study reported that ‘Hanfu’ autotetraploid apple displayed higher C. gloeosporioides resistance than ‘Hanfu’ apple, and the expression level of mdm-MIR390b was significantly upregulated in autotetraploid plants compared to that in ‘Hanfu’ plants, as demonstrated by digital gene expression (DGE) analysis. It is still unclear, however, whether mdm-MIR390b regulates apple anthracnose resistance. Apple MIR390b was transformed into apple ‘GL-3′ plants to identify the functions of mdm-MIR390b in anthracnose resistance. C. gloeosporioides treatment analysis indicated that the overexpression of mdm-MIR390b reduced fungal damage to apple leaves and fruit. Physiology analysis showed that mdm-MIR390b increased C. gloeosporioides resistance by improving superoxide dismutase (SOD) and peroxidase (POD) activity to alleviate the damage caused by O2− and H2O2. Our results demonstrate that mdm-MIR390b can improve apple plants’ anthracnose resistance.
The Green Leaf Volatile (Z)-3-Hexenyl Acetate Is Differently Emitted by Two Varieties of Tulbaghia violacea Plants Routinely and after Wounding
Authors: Frontini Alessandro , De Bellis Luigi , Luvisi Andrea , Blando Federica , Allah Samar Min , Dimita Rosanna , Mininni Carlo , Accogli Rita , Negro Carmine
Published: November 2022

While studying aromas produced by the edible flowers of Tulbaghia violacea, we noticed a different production of (Z)-3-Hexenyl acetate (a green-leaf volatile, GLV) by purple (var. ‘Violacea’) and white (var. ‘Alba’) flowers. The white Tulbaghia flowers constantly emits (Z)-3-Hexenyl acetate, which is instead produced in a lower amount by the purple-flowered variety. Thus, we moved to analyze the production of (Z)-3-Hexenyl acetate by whole plants of the two varieties by keeping them confined under a glass bell for 5 h together with a SPME (Solid Phase Micro Extraction) fiber. Results show that six main volatile compounds are emitted by T. violacea plants: (Z)-3-Hexenyl acetate, benzyl alcohol, nonanal, decanal, (Z)-3-Hexenyl-α-methylbutyrate, and one unknown compound. By cutting at half-height of the leaves, the (Z)-3-Hexenyl acetate is emitted in high quantities from both varieties, while the production of (Z)-3-Hexenyl-α-methylbutyrate increases. (Z)-3-Hexenyl acetate is a GLV capable of stimulating plant defenses, attracting herbivores and their natural enemies, and it is also involved in plant-to-plant communication and defense priming. Thus, T. violacea could represent a useful model for the study of GLVs production and a ‘signal’ plant capable of stimulating natural defenses in the neighboring plants.
Chemical Constituents of the Roots of Polygala tenuifolia and Their Anti-Inflammatory Effects
Authors: Son So-Ri , Yoon Young-Seo , Hong Joon-Pyo , Kim Jae-Min , Lee Kyung-Tae , Jang Dae Sik
Published: November 2022

Increasing scientific evidence has demonstrated that the roots of Polygala tenuifolia Willd. have pharmacological effects related to anti-inflammation. Therefore, the aim of this study is to investigate the chemical constituents from P. tenuifolia roots as anti-inflammatory drug candidates. In the present work, twenty-three compounds were isolated from P. tenuifolia roots, including three saponins (1–3), ten phenylpropanoid sucrose esters (4–12), one benzoic acid sugar ester derivative (13), four xanthones (14–17), two hydroxy benzophenone derivatives (18 and 19), two phenolic derivatives (20 and 21), and two ionones (22 and 23). All isolates were tested for their inhibitory effects of LPS-stimulated NO and PGE2 production in RAW 264.7 macrophages. Among these, 3-O-(3,4,5-trimethoxy-cinnamoyl),6′-O-(p-methoxybenzoyl) sucrose ester (TCMB; 11) together with compounds 3 and 21 exhibited significant inhibitory effects on NO production, while TCMB and compounds 17, 19, and 21 showed strong inhibitory effects on PGE2 production. Specifically, TCMB (11) downregulated the protein levels of iNOS and COX-2 in LPS-induced RAW 264.7 macrophages. In addition, TCMB (11) dose-dependently diminished the relative mRNA expression levels of iNOS, PGE2, and proinflammatory cytokines (TNF-α, IL-1β, and IL-6). A molecular docking study showed that TCMB (11) has strong binding affinities with iNOS and COX-2.
Vanilla beyond Vanilla planifolia and Vanilla × tahitensis: Taxonomy and Historical Notes, Reproductive Biology, and Metabolites
Authors: de Oliveira Renatha Tavares , da Silva Oliveira Joana Paula , Macedo Andrea Furtado
Published: November 2022

Vanilla is a worldwide cherished condiment, and its volatile market is associated with the so-called “vanilla crisis”. Even though only two species (Vanilla planifolia and V. × tahitensis) are cultivated on a large scale for commercial purposes, the Vanilla genus is comprised of 140 species. The present review article discusses the facets of this crisis, and vanilla crop wild relatives (WRs) are showcased as alternatives to overcome them. Historical, taxonomic, and reproductive biology aspects of the group were covered. Emphasis was given to the metabolic characterization of the vanilla crop WRs, highlighting their main chemical classes and the potential flavor descriptors. Many of these species can produce important flavor compounds such as vanillin, vanillic acid, and acetovanillone, among others. Overall, this review compiles valuable information that can help unravel new chapters of the history of this treasured product by evidencing the biotechnological potential of vanilla crop WRs.
Hyperhydricity in Plant Tissue Culture
Authors: Polivanova Oksana B. , Bedarev Vladislav A.
Published: November 2022

Hyperhydricity is the most common physiological disorder in in vitro plant cultivation. It is characterized by certain anatomical, morphological, physiological, and metabolic disturbances. Hyperhydricity significantly complicates the use of cell and tissue culture in research, reduces the efficiency of clonal micropropagation and the quality of seedlings, prevents the adaptation of plants in vivo, and can lead to significant losses of plant material. This review considers the main symptoms and causes of hyperhydricity, such as oxidative stress, impaired nitrogen metabolism, and the imbalance of endogenous hormones. The main factors influencing the level of hyperhydricity of plants in vitro are the mineral and hormonal composition of a medium and cultivation conditions, in particular the aeration of cultivation vessels. Based on these factors, various approaches are proposed to eliminate hyperhydricity, such as varying the mineral and hormonal composition of the medium, the use of exogenous additives, aeration systems, and specific lighting. However, not all methods used are universal in eliminating the symptoms of hyperhydricity. Therefore, the study of hyperhydricity requires a comprehensive approach, and measures aimed at its elimination should be complex and species-specific.
Plasma Electrolytic Polishing of Porous Nitinol Structures
Authors: Navickaitė Kristina , Roßmann Karl , Nestler Klaus , Böttger-Hiller Falko , Penzel Michael , Grund Thomas , Lampke Thomas , Zeidler Henning
Published: November 2022

In this study, for the first time, the application of plasma electrolytic polishing (PEP) of porous Nitinol structures, mimicking a trabecular bone structure, that were additively manufactured, is reported. The cube-shaped samples were polished in a diagonal position three different times. The effect of PEP was evaluated in terms of the polishing depth, the effect on sample chemical composition and a possible shift of the phase transition temperature using microscopy, the energy dispersive X-ray spectroscopy (EDX), and the differential scanning calorimetry (DSC) techniques, respectively. The obtained results demonstrated that the PEP technique is suitable for polishing porous structures up to a certain depth into the sample inner structure and does not have any influence on the chemical composition and the phase transformation temperatures. However, small changes in the specific enthalpy were observable among the investigated samples. These changes could be attributed to the sample chemical inhomogeneity, measurement error, and/or differences in sample size and shape.
Development of Bisphenol-A-Glycidyl-Methacrylate- and Trimethylolpropane-Triacrylate-Based Stereolithography 3D Printing Materials
Authors: Choi Yura , Yoon Jisun , Kim Jinyoung , Lee Choongjae , Oh Jaesang , Cho Namchul
Published: November 2022

The main advantages of the three-dimensional (3D) printing process are flexible design, rapid prototyping, multi-component structures, and minimal waste. For stereolithography (SLA) 3D printing, common photocurable polymers, such as bisphenol-A glycidyl methacrylate (Bis-EMA), trimethylolpropane triacrylate (TMPTMA), as well as urethane oligomers, have been widely used. For a successful 3D printing process, these photocurable polymers must satisfy several requirements, including transparency, a low viscosity, good mechanical strength, and low shrinkage post-ultraviolet curing process. Herein, we investigated SLA-type photocurable resins prepared using Bis-EMA, TMPTMA, and urethane oligomers. The flexural strength, hardness, conversion rate, output resolution, water absorption, and solubility of the printed materials were investigated. The degree of conversion of the printed specimens measured by infrared spectroscopy ranged from 30 to 60%. We also observed that 64–80 MPa of the flexural strength, 40–60 HV of the surface hardness, 15.6–29.1 MPa of the compression strength, and 3.3–14.5 MPa of the tensile strength. The output resolution was tested using three different structures comprising a series of columns (5–50 mm), circles (0.6–6 mm), and lines (0.2–5 mm). In addition, we used five different pigments to create colored resins and successfully printed complex models of the Eiffel Tower. The research on resins, according to the characteristics of these materials, will help in the design of new materials. These results suggests that acrylate-based resins have the potential for 3D printing.
Biopolymer-Based Films Reinforced with Green Synthesized Zinc Oxide Nanoparticles
Authors: Abdullah Johar Amin Ahmed , Jiménez-Rosado Mercedes , Guerrero Antonio , Romero Alberto
Published: November 2022

Nowadays, biopolymer-based films are being developed as an alternative to conventional plastic-based films, mainly because they are non-toxic, flexible, inexpensive, and widely available. However, they are restricted in their applications due to several deficiencies in their properties. Accordingly, the reinforcement of these materials with nanoparticles/nanofillers could overcome some of their shortcomings, especially those processed by green methods. Green synthesized zinc oxide nanoparticles (ZnO-NPs) are highly suggested to overcome these deficiencies. Therefore, the main aim of this work was to develop different biopolymer-based films from cellulose acetate (CA), chitosan (CH), and gelatin (GE) reinforced with ZnO-NPs prepared by casting, and to assess their different properties. The results show the improvements produced by the incorporation of ZnO-NPs (1% w/w) into the CA, CH, and GE systems. Thus, the water contact angles (WCAs) increased by about 12, 13, and 14%, while the water vapor permeability (WVP) decreased by about 14, 6, and 29%, the water solubility (WS) decreased by about 23, 6, and 5%, and the transparency (T) increased by about 19, 31, and 20% in the CA, CH, and GE systems, respectively. Furthermore, the mechanical properties were enhanced by increasing the ultimate tensile strength (UTS) (by about 39, 13, and 26%, respectively) and Young’s modulus (E) (by about 70, 34, and 63%, respectively), thereby decreasing the elongation at the break (εmax) (by about 56, 23, and 49%, respectively) and the toughness (by about 50, 4, and 30%, respectively). Lastly, the antioxidant properties were enhanced by 34, 49, and 39%, respectively.
Antimicrobial and Gas Barrier Crustaceans and Fungal Chitin-Based Coatings on Biodegradable Bioplastic Films
Authors: Panariello Luca , Coltelli Maria-Beatrice , Hadrich Ahdi , Braca Francesca , Fiori Stefano , Haviv Amit , Miketa Filip , Lazzeri Andrea , Staebler Andreas , Gigante Vito , Cinelli Patrizia
Published: November 2022

Chitin nanofibrils (CN) can be obtained from crustaceans and fungal sources and can be used for preparing coatings for bioplastic films, that are fundamental for developing a safe and sustainable biodegradable food packaging. Coatings with different concentrations of CN from shrimps were applied on different bioplastic substrates, like Poly (butylene succinate-co-adipate)/Poly(3-hydroxybutyrate-co-3-hydroxyvalerate (PBSA/PHBV) blend, Polybutylene succinate (PBS), and Polybutylene adipate terephthalate/Poly(lactic acid) (PBAT/PLA) blend, but the adhesion to the substrates was scarce. On the contrary, the fungal-based CN showed a better adhesion. Additionally, it was found that the use of an additive based on oligomeric lactic acid was useful to prepare a coating with an improved adhesion to bioplastics. The gas barrier properties to oxygen and water vapour of coated and un-coated films were measured, revealing an improvement of these properties thanks to applied coatings, especially towards the oxygen. Antimicrobial properties and biodegradation capacity were also evaluated revealing an antibacterial effect of the coatings that did not significantly interfere with their biodegradability. The results are discussed and interpreted considering the correlation between composition and macromolecular structures with the observed functional properties.
Modification Strategies for Ionic Complementary Self-Assembling Peptides: Taking RADA16-I as an Example
Authors: Guo Weiwei , Ma Yinping , Hu Lei , Feng Yujie , Liu Yanmiao , Yi Xuedong , Zhang Wenzhi , Tang Fushan
Published: November 2022

Ion-complementary self-assembling peptides have been studied in many fields for their distinct advantages, mainly due to their self-assembly properties. However, their shortcomings, such as insufficient specific activity and poor mechanical properties, also limited their application. For the better and wider application of these promising biomaterials, ion-complementary self-assembling peptides can be modified with their self-assembly properties not being destroyed to the greatest extent. The modification strategies were reviewed by taking RADA16-I as an example. For insufficient specific activity, RADA16-I can be structurally modified with active motifs derived from the active domain of the extracellular matrix or other related active factors. For weak mechanical properties, materials with strong mechanical properties or that can undergo chemical crosslinking were used to mix with RADA16-I to enhance the mechanical properties of RADA16-I. To improve the performance of RADA16-I as drug carriers, appropriate adjustment of the RADA16-I sequence and/or modification of the RADA16-I-related delivery system with polymer materials or specific molecules can be considered to achieve sustained and controlled release of specific drugs or active factors. The modification strategies reviewed in this paper may provide some references for further basic research and clinical application of ion-complementary self-assembling peptides and their derivatives.
Design of Soft-Sensing Model for Alumina Concentration Based on Improved Deep Belief Network
Authors: Li Xiangquan , Liu Bo , Qian Wei , Rao Guoyong , Chen Lijuan , Cui Jiarui
Published: November 2022

Alumina concentration is an important parameter in the production process of aluminum electrolysis. Due to the complex production environment in the industrial field and the complex physical and chemical reactions in the aluminum reduction cell, nowadays it is still unable to carry out online measurement and real-time monitoring. For solving this problem, a soft-sensing model of alumina concentration based on a deep belief network (DBN) is proposed. However, the soft-sensing model may have some limitations for different cells and different periodic working conditions such as local anode effect, pole changing, and bus lifting in the same cell. The empirical mode decomposition (EMD) and particle swarm optimization (PSO) with the DBN are combined, and an EMD–PSO–DBN method that can denoize and optimize the model structure is proposed. The simulation results show that the improved soft-sensing model improves the accuracy and universality of prediction.
Applicability of Convolutional Neural Network for Estimation of Turbulent Diffusion Distance from Source Point
Authors: Ishigami Takahiro , Irikura Motoki , Tsukahara Takahiro
Published: November 2022

For locating the source of leaking gas in various engineering fields, several issues remain in the immediate estimation of the location of diffusion sources from limited observation data, because of the nonlinearity of turbulence. This study investigated the practical applicability of diffusion source-location prediction using a convolutional neural network (CNN) from leaking gas instantaneous distribution images captured by infrared cameras. We performed direct numerical simulation of a turbulent flow past a cylinder to provide training and test images, which are scalar concentration distribution fields integrated along the view direction, mimicking actual camera images. We discussed the effects of the direction in which the leaking gas flows into the camera’s view and the distance between the camera and the leaking gas on the accuracy of inference. A single learner created by all images provided an inference accuracy exceeding 85%, regardless of the inflow direction or the distance between the camera and the leaking gas within the trained range. This indicated that, with sufficient training images, a high-inference accuracy can be achieved, regardless of the direction of gas leakage or the distance between the camera and the leaking gas.
Conceptualization of an Anthropomorphic Replacement Hand with a Sensory Feedback System
Authors: Allmendinger Lea , Hazubski Simon , Otte Andreas
Published: November 2022

In this paper, a concept for an anthropomorphic replacement hand cast with silicone with an integrated sensory feedback system is presented. In order to construct the personalized replacement hand, a 3D scan of a healthy hand was used to create a 3D-printed mold using computer-aided design (CAD). To allow for movement of the index and middle fingers, a motorized orthosis was used. Information about the applied force for grasping and the degree of flexion of the fingers is registered using two pressure sensors and one bending sensor in each movable finger. To integrate the sensors and additional cavities for increased flexibility, the fingers were cast in three parts, separately from the rest of the hand. A silicone adhesive (Silpuran 4200) was examined to combine the individual parts afterwards. For this, tests with different geometries were carried out. Furthermore, different test series for the secure integration of the sensors were performed, including measurements of the registered information of the sensors. Based on these findings, skin-toned individual fingers and a replacement hand with integrated sensors were created. Using Silpuran 4200, it was possible to integrate the needed cavities and to place the sensors securely into the hand while retaining full flexion using a motorized orthosis. The measurements during different loadings and while grasping various objects proved that it is possible to realize such a sensory feedback system in a replacement hand. As a result, it can be stated that the cost-effective realization of a personalized, anthropomorphic replacement hand with an integrated sensory feedback system is possible using 3D scanning and 3D printing. By integrating smaller sensors, the risk of damaging the sensors through movement could be decreased.
Polarimetric Quantum-Strong Correlations with Independent Photons on the Poincaré Sphere
Authors: Vatarescu Andre
Published: November 2022

Controllable, quantum-strong correlations of polarization states can be implemented with multi-photon independent states. Polarization-based photonic quantum correlations can be traced back to the overlap of the polarization Stokes vectors on the Poincaré sphere between two polarization filters. The quantum Rayleigh scattering prevents a single photon from propagating in a straight line inside a dielectric medium, and it also provides a mechanism for the projective measurement of polarization. Complexities associated with single-photon sources and detectors can be eliminated because the quantum Rayleigh scattering in a dielectric medium destroys entangled photons. Entanglement-free, identical sources and processing devices give rise to correlations rather than these being caused by “quantum nonlocality”. These analytic developments were prompted by the vanishing expectation values of the Pauli spin vector for a single photon of maximally entangled photonic Bell states.
“Blessed Is the One Whose Bowels Can Move: An Essay in Praise of Lament” in Contemporary Worship
Authors: Sigmon Casey T.
Published: November 2022

The CCLI charts may not reflect it, yet one thing many Christian churches discovered as the pandemic raged across the world (and violence at home and abroad) was the need for songs of sacred lament. Unfortunately, many churchgoers, especially those who identify as practitioners of contemporary Christian worship, have cultivated a gap between the biblical give and take of praise and lament revealed most poignantly in the book of Psalms. This chasm between praise and lament is a problem, as a liturgical discourse about disastrous events is weakened. Churches sing congregational songs of praise in the church, the chorus of ‘what ought to be’. Meanwhile, outside the church, artists in genres as diverse as folk and rap sing the chorus of what frankly ‘is’. For the church to be transformative, it must be grounded in what is (lament) and aiming toward what ought to be (praise). This is the value of the cycle of praise and lament in the church’s liturgy. This article explores the impact of CCM (contemporary Christian music) and praise and worship culture as it laments the loss of lament in Christian worship. The essay articulates the missing sense of ‘Truth’ in contemporary congregational music, as defined by Don Saliers’ Worship Come to Its Senses. The article closes by amplifying emerging Christian songwriters reintroducing lament to contemporary worship.
Effect and Regulation of Obesity-Associated Low-Grade Chronic Inflammation in Major Rheumatic Diseases
Authors: Alonso-Pérez Ana , Guillán-Fresco María , López-Fagúndez Miriam , Pazos-Pérez Andrés , Crespo-Golmar Antía , Piñeiro-Ramil María , López Verónica , Jorge-Mora Alberto , Gómez Rodolfo
Published: November 2022

Current lifestyle and environmental factors contribute to obesity development, leading to low-grade chronic inflammation (LGCI). Apart from obesity, LGCI is also related to rheumatic diseases such as osteoporosis (OP) and osteoarthritis (OA). In these, an excessive accumulation of adipose tissue has been linked to an excessive production of proinflammatory factors, such as adipokines. This work’s aim is to stablish the effect of obesity-associated LGCI in major rheumatic diseases and to determine optimal strategies to reduce it. Obesity is a risk factor for developing OA, where a systemic LGCI state has been found. Concretely, obesity-associated LGCI has been described as an OA instauration and progression promoter. To avoid this, several therapeutical approaches (diet control, physical exercise, or nutraceuticals) have been tested. OP is another major rheumatic disease where a basal LGCI has been described, being worsened by obesity. As in OA, diet management and supplementation with vitamin D or probiotics have been proposed as approaches to treat obesity-associated LGCI in this pathology. Currently, the increase in the prevalence of rheumatic diseases is unstoppable. Nonetheless, obesity is a risk factor that can be controlled. Thus, the study of new interventions to control the impact of obesity-associated LGCI is a challenge for the management of patients with rheumatic diseases.
Spectral Expansions for Credit Risk Modelling with Occupation Times
Authors: Campolieti Giuseppe , Kato Hiromichi , Makarov Roman N.
Published: November 2022

We study two credit risk models with occupation time and liquidation barriers: the structural model and the hybrid model with hazard rate. The defaults within the models are characterized in accordance with Chapter 7 (a liquidation process) and Chapter 11 (a reorganization process) of the U.S. Bankruptcy Code. The models assume that credit events trigger as soon as the occupation time (the cumulative time the firm’s value process spends below some threshold level) exceeds the grace period (time allowance). The hazard rate model extends the structural occupation time models and presumes that other random factors may also lead to credit events. Both approaches allow the firm to fulfill its obligations during the grace period. We derive new closed-from pricing formulas for credit derivatives containing the (risk-neutral) probability of defaults and credit default swap (CDS) spreads as special cases, which are derived analytically via a spectral expansion methodology. Our method works for any solvable diffusion, such as the geometric Brownian motion (GBM) and several state-dependent volatility processes, including the constant elasticity of variance (CEV) model. It allows us to write the pricing formulas explicitly as infinite series that converges rapidly. We then calibrate our models (assuming that GBM governs the firm’s value) to market CDS spreads from the Total Energy company. Our calibration results show that the computations are fast, and the fit is near-perfect.
Singularity Analysis and Complete Methods to Compute the Inverse Kinematics for a 6-DOF UR/TM-Type Robot
Authors: Villalobos Jessica , Sanchez Irma Y. , Martell Fernando
Published: November 2022

Improving the strategies employed to control robotic arms is of great importance because of the increase in their use in advanced supervisory control strategies, such as digital twins. The inverse kinematic (IK) control of manipulators requires an IK solution and an awareness of the singular configurations. This work presents a complete IK calculation system with singularity analysis for the UR5 robotic arm created by Universal Robots. For a specific robot pose, different angle solution sets are obtained, and one of these solution sets has to be selected to achieve movement continuity and avoid singularities. Two methods for this double purpose are proposed: one calculates all the solution possibilities, and the other obtains only one solution set by following a sequence of decisions and calculations clearly stated by a finite state machine (FSM). Both methods are effective in managing singularities. The FSM-based method complements the IK solution procedure with advantages in the number of computations and performance by producing results that would not lead the joints to move abruptly. The results prove that the presented methods select an IK solution that does not result in a singular configuration, and that most of the time, they lead to the same valid IK solution.
Impacts of 3DEnVar-Based FY-3D MWHS-2 Radiance Assimilation on Numerical Simulations of Landfalling Typhoon Ampil (2018)
Authors: Song Lixin , Shen Feifei , Shao Changliang , Shu Aiqing , Zhu Lijian
Published: November 2022

The module for assimilating radiance data of the Microwave Humidity Sounder-2 (MWHS-2) onboard the Feng Yun 3D (FY-3D) satellite is built in the Weather Research and Forecasting (WRF) model data assimilation (WRFDA) system. The CONV, 3DVar, and EnVar experiments are conducted to investigate the impact of assimilating the new humidity sounder based on Typhoon Ampil (2018). Both the 3DVar and EnVar experiments assimilate FY-3D MWHS-2 radiance data on top of the conventional data, while the CONV experiment only applies conventional data. In the EnVar experiment, notable geopotential height increment is observed around the typhoon, leading the typhoon to move northeast. In addition, the moisture field is improved to some extent. Finally, from the analysis of the dynamic field of the typhoon, it can be found that the EnVar experiment can adjust the dynamic structure of the typhoon. Furthermore, the assimilation of FY-3D MWHS-2 radiance data reduces the forecast error of the typhoon track and intensity. Additionally, the precipitation skill is improved in terms of rainfall pattern and the verification score. This improvement in the precipitation may be closely related to the features of the circulation structure concerning the evolution of the typhoon. The improved prediction of the position and intensity of rainbands in the FY-3D MWHS-2 radiance data assimilation experiment corresponds to a better prediction of typhoon structure.
Integrating Real Tree Skeleton Reconstruction Based on Partial Computational Virtual Measurement (CVM) with Actual Forest Scenario Rendering: A Solid Step Forward for the Realization of the Digital Twins of Trees and Forests
Authors: Wang Zhichao , Lu Xin , An Feng , Zhou Lijun , Wang Xiangjun , Wang Zhihao , Zhang Huaiqing , Yun Ting
Published: November 2022

Digital twins of forests (trees) are computational virtual recreations of forests (trees) in which the entity distributions and physical processes in real-world forests (trees) are duplicated. It is expected that conventional forest science and management can be undertaken in a digital twin of forests (trees) if the recreation of a real-world forest (tree) has accurate and comprehensive enough information. However, due to the various differences between the current tree model and the real tree, these envisioned digital twins of the forests (trees) stay a theoretical concept. In this study, we developed a processing strategy that partially integrated computational virtual measurement (CVM) process into the tree modeling workflow. Owing to the feature of CVM, partial tree skeleton reconstruction procedures were considered to have higher mechanical objectivity compared to conventional mathematical modeling methods. The reason was that we developed a novel method called virtual diameter tape (VDT), which could provide a certain percentage of modeling elements using CVM. Technically, VDT was able to virtually measure diameters and spatial distribution of cross-sectional area of trees, including the basal area, from point clouds. VDT simulated the physical scenario of diameter tapes, observing point clouds of trees. Diameter and the cross-sectional area of stem and branches were obtained by two consecutive physical measurement processes, one in the forest sample site and another in the virtual space. At the same time, VDT obtained better or a similar accuracy compared to the mathematical methods, i.e., Hough transform-based methods, using the same data sets. The root-mean-square deviation (RMSE) of retrieval of diameter at breast height (DBH) using VDT was 1.02 cm, while DBH obtained from three conventional methods varied from 1.29 cm to 1.73 cm. Based on VDT measurement results, tree skeleton reconstruction and actual forest scenario rendering of our sample plots were further implemented. Beyond the visual consistency, we believe that our work might be a small and solid step in the technological evolution from tree models to the digital twin of forests (trees).