bluuebunny/crossref_metadata_2025 huggingface dataset:
Sonographic evaluation of patellar tendon displacement and its correlation with patellofemoral pain syndrome
Authors: Gonzalez-Suarez Consuelo B , Apiag Cherie-Lee A , Agarao Kris A. , Chavez Fe T. , Espino Reil V. , Sibayan Saul A. , Serra Mark A. , Sosa Ken E. , Gomez Ivan N.
Published: October 2021

Background

Patellofemoral pain syndrome is the most common knee condition and is associated with patellar maltracking. Ultrasound is used in studying patellar maltracking. The objective is to determine if the technique which analyzes the displacement of the patellar tendon in the trochlear sulcus is associated with patellofemoral pain syndrome.

Methods

In total, 68 knees of 34 football players (males = 20, females = 14) were included. Patellar tendon displacement was assessed in supine and standing positions. Patellar tendon displacement difference in the two positions was determined.

Results

There was a significant difference in the lateral patellar tendon displacement during standing which was larger in patellofemoral pain syndrome than without patellofemoral pain syndrome (Mean Rank = 39.20 vs. 30.32, p = 0.02). There was no significant difference between the two groups for lateral patellar tendon displacement in supine and the difference in patellar tendon displacement from supine to standing.

Conclusion

The technique could be a potential method in assessing patellar maltracking. It could be used to have a comprehensive understanding of the pathomechanics and treatment of patellofemoral pain syndrome.
Electricity Consumers’ Satisfaction During COVID-19 Lockdown in Some Selected States in Nigeria
Authors: Lawal Muyideen O. , Ajewole Titus O. , Bada Olalekan M.
Published: October 2021

This work studies the satisfaction experienced by electricity customers in Osun, Lagos, and Ogun States during the recent lockdown occasioned by the COVID-19 pandemic. The measures of the satisfaction are based on the hours of electricity supply during the period, easiness experienced for payment of prepaid bill and swiftness in faults correction of the distribution companies involved. A questionnaire was developed using Google Forms. The form was shared through various online platforms. A total of 274 electricity consumers responded to the questionnaire, but after sorting, 259 consumers across the three states were analyzed. From the responses, majority of respondents claim there was increased hours of supply during the period when compared to before the period. For prepaid customers who had reasons to pay electricity bill, majority claim it was easy paying while most of those who claimed it was not easy was because of their payment method choices, which is using designated points. Majority of those who have reasons for faults to be corrected by the distribution companies claim that there was not promptness in fault correction.
Fanconi Syndrome Induced by Concomitant HIV PrEP and Tacrolimus
Authors: Linn Becky S. , Vandiver Jeremy W. , Ren Dylan , Shassetz Joseph
Published: October 2021

Fanconi syndrome (FS) is a severe grade of drug-induced proximal tubule toxicity. There are numerous causes for acquired FS, and drug toxicity is one of the most common. FS is known to be associated with the nucleoside reverse transcriptase inhibitor (NRTI) tenofovir disoproxil fumarate (TDF). TDF is often used in combination with emtricitabine (FTC) for preexposure prophylaxis (PrEP) of human immunodeficiency virus (HIV) infection. TDF/FTC-induced FS has been observed as a dose-related phenomenon that is directly correlated to kidney function, high levels of absorption of the drug into the proximal tubule, and interactions with other medications. This case report describes a patient who acquired FS after starting TDF/FTC for PrEP in the setting of chronic kidney disease (CKD) with concomitant tacrolimus therapy, a known nephrotoxic agent.
Psychometric Analysis of the Professional Identity Questionnaires in Croatian Nursing Student Context
Authors: Gusar Ivana , Tokić Andrea , Lovrić Robert
Published: October 2021

Introduction: The identification of nursing students with their profession, as well as gained professional competencies, are the crucial factors of the quality of their future work activities and the level of safety of the patient they are taking care of. Therefore, it is of utter importance to estimate the nursing students’ professional identity during their education. Objectives: The purpose of this study was to translate Professional Identity Five-Factor Scale and Identity questionnaire, to test their psychometric properties in Croatian practice and to identify more suitable instrument of measuring the level of nursing students' professional identity in Croatian context. Methods: Quantitative, cross-sectional two-phase study was performed. In the first phase translated questionnaires were administered to a sample of 432 nursing students. Based on the confirmatory factor analysis (CFA), which revealed poor model fit, the principal component analysis was conducted. In the second phase, CFA was used to test the Croatian version of the instruments' factor structure on a sample of 222 students with different study orientations. Results: The CFA results, conducted in first phase, do not support the original version of the factor structures of the Professional Identity Five-Factor Scale and Identity models. The exploratory approach showed that the translated Professional Identity Five-Factor Scale had three factors with good psychometric properties explaining 49.35% of the variance. Translated Identity questionnaire had a two-factor structure (63.46% explained variance) with good reliability properties. In the second phase, on another heterogeneous sample of students, the described factor structure was found to have a good model fit obtained by CFA. Conclusions: According to the findings, Professional Identity Five-Factor is more appropriate for evaluating professional identity of nursing students, and Identity is probably better suited to measure professional identity among working professionals.
Deciphering Links Between Sexual Violence and Castes in India
Authors: Musahar Yogendra
Published: October 2021

The recent incident, the gang rape and murder of a 19-year-old woman in Hathras, a small village in Uttar Pradesh of India, once again sparks a debate on links between sexual violence and castes in India. This article aims to examine the links between sexual violence and castes in India. This study utilizes the national representative National Family Health Survey 4 (NFHS-4, 2015–16) data. A bivariate analysis was carried out to analyse the data. A binary logistic regression model was applied to predict the effect of explanatory variables, viz. type of place of residence, years of schooling complete, economic status in terms of wealth index and finally castes on predicted variable, i.e. sexual violence. The binary regression model indicates that there were links between sexual violence and castes. For secured and dignified life of women, caste-based sexual violence must be annihilated.
Poverty and Education: Attainments and Challenges for Tribal Communities
Authors: Rupavath Ramdas
Published: October 2021

Various educational policies and government initiatives at the national level in India have aimed at improving the literacy rate of the tribal communities. However, even after 73 years of independence, the literacy levels of the tribal people have not risen to the desired levels. On top, there is the issue of high dropout rates (Ministry of Tribal Affairs, Statistics Division, Government of India, 2013, Statistical Profile of Scheduled Tribes in India, https://tribal.nic.in/ST/StatisticalProfileofSTs2013.pdf ; 70.9%, according to a recent study) among the Tribals (Rupavath, 2016a, Review Pub Administration Management, 4, p. 183). In such a situation, it is important to find out why tribal communities are still lagging behind in the education sector.

This study will deal with the various aspects of access to education for the tribal communities. It will also try to examine the linkage between poverty and education. This is considered important since literacy can mean much more than mere bookish knowledge. A literate person can be expected to have more awareness about matters of importance to him or her. On the employment front, a literate person can have wider livelihood options—and not be confined to pursue occupations which largely entail manual labour. At the same time, a literate person would know more about his or her legitimate entitlements and be in a better position to avail of these. It is one thing for the government to reserve vacancies for candidates belonging to the deprived sections of society. It is equally important for more persons hailing from these sections to know about these and utilize these. Education can be a means for such persons to optimally utilize the opportunities available to them.

It is a proven fact that students learn better and retain more when they are active participants in the teaching–learning process. For the purpose of this study, two districts—Anantapur and Vishakhapatnam (in Andhra Pradesh), and Hyderabad district (in Telangana)—have been selected for a detailed examination.

The study will include not only the availability of infrastructure but also participation of the tribal students in the classroom discussions. The study will utilize both purposive and random sampling methods.
The Evolution of Drug Information Services to Asynchronous Delivery of Pharmacist eConsults
Authors: Clinard Valerie , Stebbins Marilyn , Lynch Shalini
Published: October 2021

Drug information (DI) services provided an avenue to expand the role of pharmacists as the medication experts. The focus of DI has shifted from general questions submitted to DI centers to patient-specific questions that optimize care. One method to increase access to pharmacist expertise is through pharmacy eConsults. Pharmacy eConsults provide specialist care for medically complex patients using a patient-centered, asynchronous approach. The purpose of this article is to describe the evolution of consults from formal drug information services and describe one academic medical center’s implementation of a pharmacy eConsult service to provide patient-specific DI.
Type 1 interferon to prevent leukemia relapse after allogeneic transplantation
Authors: Magenau John M. , Peltier Dan , Riwes Mary , Pawarode Attaphol , Parkin Brian , Braun Thomas , Anand Sarah , Ghosh Monalisa , Maciejewski John , Yanik Gregory , Choi Sung Won , Talpaz Moshe , Reddy Pavan
Published: October 2021

A potent graft-versus-leukemia (GVL) response is crucial in preventing relapse, the major impediment to successful allogeneic hematopoietic cell transplantation (HCT). In preclinical studies, type 1 interferon (IFN-α) enhanced cross-presentation of leukemia-specific antigens by CD8α dendritic cells (DCs) and amplified GVL. This observation was translated into a proof-of-concept phase 1/2 clinical trial with long-acting IFN-α (pegylated IFN-α [pegIFNα]) in patients undergoing HCT for high-risk acute myeloid leukemia (AML). Patients with treatment-resistant AML not in remission or those with poor-risk leukemia were administered 4 dosages of pegIFNα every 14 days beginning at day −1 before HCT. Dose selection was established by adaptive design that continuously assessed the probability of dose-limiting toxicities throughout the trial. Efficacy was evaluated by determining the 6-month incidence of relapse at the maximum tolerated dose (MTD). Thirty-six patients (median age, 60 years) received pegIFNα treatment. Grade 3 or greater severe adverse events occurred in 25% of patients, establishing 180 μg as the MTD. In phase 2, the incidence of relapse was 39% at 6 months, which was sustained through 1-year post-HCT. The incidence of transplant-related mortality was 13%, and severe grade III-IV acute graft-versus-host disease (GVHD) occurred in 11%. Paired blood samples from donors and recipients after HCT revealed elevated levels of type 1 IFN with cellular response, the persistence of cross-presenting DCs, and circulating leukemia antigen-specific T cells. These data suggest that prophylactic administration of pegIFNα is feasible in the peri-HCT period. In high-risk AML, increased toxicity was not observed with preliminary evidence for reduction in leukemia relapse after HCT. This trial was registered at www.clinicaltrials.gov as #NCT02328755.
Multiple breath washout: measuring early manifestations of lung pathology
Authors: Stanojevic Sanja , Bowerman Cole , Robinson Paul
Published: October 2021

The multiple breath washout (MBW) test measures the efficiency of gas mixing in the lungs and has gained significant interest over the past 20 years. MBW outcomes detect early lung function impairment and peripheral airway pathology, through its main outcome measure lung clearance index (LCI). LCI measures the number of lung turnovers required to washout an inert tracer gas. MBW is performed during normal (tidal) breathing, making it particularly suitable for young children or those who have trouble performing forced manoeuvres. Additionally, research in chronic respiratory disease populations has shown that MBW can detect acute clinically relevant changes before conventional lung function tests, such as spirometry, thus enabling early intervention. The development of technical standards for MBW and commercial devices have allowed MBW to be implemented in clinical research and potentially routine clinical practice. Although studies have summarised clinimetric properties of MBW indices, additional research is required to establish the clinical utility of MBW and, if possible, shorten testing time. Sensitive, feasible measures of early lung function decline will play an important role in early intervention for people living with respiratory diseases.

Educational aim

To describe the multiple breath washout test, its applications to lung pathology and respiratory disease, as well as directions for future research.
Rickettsia burneti and Brucella melitensis co-infection: a case report and literature review
Authors: Song Jiangqin , Hu Xiaorong , Li Xiaolong , Chen Youping , Yan Xiangyuan , Zhu Weifang , Ding Yan , Zhou Junyang
Published: October 2021

Rickettsia is the pathogen of Q fever, Brucella ovis is the pathogen of brucellosis, and both of them are Gram-negative bacteria which are parasitic in cells. The mixed infection of rickettsia and Brucella ovis is rarely reported in clinic. Early diagnosis and treatment are of great significance to the treatment and prognosis of brucellosis and Q fever. Here, we report a case of co-infection Rickettsia burneti and Brucella melitensis. The patient is a 49-year-old sheepherder, who was hospitalized with left forearm trauma. Three days after admission, the patient developed fever of 39.0°C, accompanied by sweating, fatigue, poor appetite and headache. Indirect immunofluorescence (IFA) was used to detect Rickettsia burneti IgM. After 72 hours of blood culture incubation, bacterial growth was detected in aerobic bottles, Gram-negative bacilli were found in culture medium smear, the colony was identified as Brucella melitensis by mass spectrometry. Patients were treated with doxycycline (100 mg bid, po) and rifampicin (600 mg qd, po) for 4 weeks. After treatment, the symptoms disappeared quickly, and there was no sign of recurrence or chronic infection. Q fever and Brucella may exist in high-risk practitioners, so we should routinely detect these two pathogens to prevent missed diagnosis.
The essential roles of OsFtsH2 in developing the chloroplast of rice
Authors: Wu Qingfei , Han Tiantian , Yang Li , Wang Qiang , Zhao Yingxian , Jiang Dean , Ruan Xiao
Published: October 2021

Background

Filamentation temperature-sensitive H (FtsH) is an ATP-dependent zinc metalloprotease with ATPase activity, proteolysis activity and molecular chaperone-like activity. For now, a total of nine FtsH proteins have been encoded in rice, but their functions have not revealed in detail. In order to investigate the molecular mechanism ofOsFtsH2here, severalosftsh2knockout mutants were successfully generated by the CRISPR/Cas9 gene editing technology.

Results

All the mutants exhibited a phenotype of striking albino leaf and could not survive through the stage of three leaves. OsFtsH2 was located in the chloroplast and preferentially expressed in green tissues. In addition,osftsh2mutants could not form normal chloroplasts and had lost photosynthetic autotrophic capacity. RNA sequencing analysis indicated that many biological processes such as photosynthesis-related pathways and plant hormone signal transduction were significantly affected inosftsh2mutants.

Conclusions

Overall, the results suggested OsFtsH2 to be essential for chloroplast development in rice.
Comparative characterization and osteogenic / adipogenic differentiation of mesenchymal stem cells derived from male rat hair follicles and bone marrow
Authors: Zaki Abdel Kader A. , Almundarij Tariq I. , Abo-Aziza Faten A. M.
Published: October 2021

Clinical applications of cell therapy and tissue regeneration under different conditions need a multiplicity of adult stem cell sources. Up to date, little is available on the comparative isolation, characterization, proliferation, rapid amplification, and osteogenic/adipogenic differentiation of rat mesenchymal stem cells (MSCs) isolated from living bulge cells of the hair follicle (HF) and bone marrow (BM) from the same animal. This work hopes to use HF-MSCs as an additional adult stem cell source for research and application. After reaching 80% confluence, the cell counting, viability %, and yields of HF-MSCs and BM-MSCs were nearly similar. The viability % was 91.41 ± 2.98 and 93.11 ± 3.06 while the cells yield of initial seeding was 33.15 ± 2.76 and 34.22 ± 3.99 and of second passage was 28.76 ± 1.01 and 29.56 ± 3.11 for HF-MSCs and BM-MSCs respectively. Clusters of differentiation (CDs) analysis revealed that HF-MSCs were positively expressed CD34, CD73 and CD200 and negatively expressed CD45. BM-MSCs were positively expressed CD73 and CD200 and negatively expressed of CD34 and CD45. The proliferation of HF-MSCs and BM-MSCs was determined by means of incorporation of Brd-U, population doubling time (PDT) assays and the quantity of formazan release. The percentage of Brd-U positive cells and PDT were relatively similar in both types of cells. The proliferation, as expressed by the quantity of formazan assay in confluent cells, revealed that the quantity of release by BM-MSCs was slightly higher than HF-MSCs. Adipogenic differentiated BM-MSCs showed moderate accumulation of oil red-O stained lipid droplets when compared to that of HF-MSCs which exhibited high stain. The total lipid concentration was significantly higher in adipogenic differentiated HF-MSCs than BM-MSCs (P < 0.05). It was found that activity of bone alkaline phosphatase and calcium concentration were significantly higher (P < 0.01 andP < 0.05 respectively) in osteogenic differentiated BM-MSCs than that of HF-MSCs. The present findings demonstrate that the HF-MSCs are very similar in most tested characteristics to BM-MSCs with the exception of differentiation. Additionally; no issues have been reported during the collection of HF-MSCs. Therefore, the HF may represent a suitable and accessible source for adult stem cells and can be considered an ideal cell source for adipogenesis research.
Positive feedback between retinoic acid and 2-phospho-L-ascorbic acid trisodium salt during somatic cell reprogramming
Authors: Zhang Mengdan , Li Qian , Yang Tingting , Meng Fei , Lai Xiaowei , Liang Lining , Li Changpeng , Sun Hao , Sun Jiaqi , Zheng Hui
Published: October 2021

Retinoic acid (RA) and 2-phospho-L-ascorbic acid trisodium salt (AscPNa) promote the reprogramming of mouse embryonic fibroblasts to induced pluripotent stem cells. In the current studies, the lower abilities of RA and AscPNa to promote reprogramming in the presence of each other suggested that they may share downstream pathways at least partially. The hypothesis was further supported by the RNA-seq analysis which demonstrated a high-level overlap between RA-activated and AscPNa activated genes during reprogramming. In addition, RA upregulated Glut1/3, facilitated the membrane transportation of dehydroascorbic acid, the oxidized form of L-ascorbic acid, and subsequently maintained intracellular L-ascorbic acid at higher level and for longer time. On the other hand, AscPNa facilitated the mesenchymal-epithelial transition during reprogramming, downregulated key mesenchymal transcriptional factors like Zeb1 and Twist1, subsequently suppressed the expression of Cyp26a1/b1 which mediates the metabolism of RA, and sustained the intracellular level of RA. Furthermore, the different abilities of RA and AscPNa to induce mesenchymal-epithelial transition, pluripotency, and neuronal differentiation explain their complex contribution to reprogramming when used individually or in combination. Therefore, the current studies identified a positive feedback between RA and AscPNa, or possibility between vitamin A and C, and further explored their contributions to reprogramming.
Cardiac cell type-specific responses to injury and contributions to heart regeneration
Authors: Zhang Weijia , Liang Jinxiu , Han Peidong
Published: October 2021

Heart disease is the leading cause of mortality worldwide. Due to the limited proliferation rate of mature cardiomyocytes, adult mammalian hearts are unable to regenerate damaged cardiac muscle following injury. Instead, injured area is replaced by fibrotic scar tissue, which may lead to irreversible cardiac remodeling and organ failure. In contrast, adult zebrafish and neonatal mammalian possess the capacity for heart regeneration and have been widely used as experimental models. Recent studies have shown that multiple types of cells within the heart can respond to injury with the activation of distinct signaling pathways. Determining the specific contributions of each cell type is essential for our understanding of the regeneration network organization throughout the heart. In this review, we provide an overview of the distinct functions and coordinated cell behaviors of several major cell types including cardiomyocytes, endocardial cells, epicardial cells, fibroblasts, and immune cells. The topic focuses on their specific responses and cellular plasticity after injury, and potential therapeutic applications.
Overexpression of HOXA9 upregulates NF-κB signaling to promote human hematopoiesis and alter the hematopoietic differentiation potentials
Authors: Zeng Jiahui , Yi Danying , Sun Wencui , Liu Yuanlin , Chang Jing , Zhu Lijiao , Zhang Yonggang , Pan Xu , Dong Yong , Zhou Ya , Lai Mowen , Bian Guohui , Zhou Qiongxiu , Liu Jiaxin , Chen Bo , Ma Feng
Published: October 2021

Background

The HOX genes are master regulators of embryogenesis that are also involved in hematopoiesis. HOXA9 belongs to a cluster of HOX genes that play extensively studied roles in hematopoiesis and leukemogenesis.

Methods

We established HOXA9-inducible human embryonic stem cells (HOXA9/hESCs) with normal pluripotency and potential for hematopoiesis, which could be used to analyze gene function with high accuracy. HOXA9/hESCs co-cultured with aorta–gonad–mesonephros-derived stromal cells (AGM-S3) were induced to overexpress HOXA9 with doxycycline (DOX) at various times after hematopoiesis started and then subjected to flow cytometry.

Results

Induction of HOXA9 from Day 4 (D4) or later notably promoted hematopoiesis and also increased the production of CD34+ cells and derived populations. The potential for myelogenesis was significantly elevated while the potential for erythrogenesis was significantly reduced. At D14, a significant promotion of S phase was observed in green fluorescent protein positive (GFP+) cells overexpressing HOXA9. NF-κB signaling was also up-regulated at D14 following induction of HOXA9 on D4. All of these effects could be counteracted by addition of an NF-κB inhibitor or siRNA against NFKB1 along with DOX.

Conclusions

Overexpression of HOXA9 starting at D4 or later during hematopoiesis significantly promoted hematopoiesis and the production of myeloid progenitors while reduced the production of erythroid progenitors, indicating that HOXA9 plays a key role in hematopoiesis and differentiation of hematopoietic lineages.
From wound response to repair – lessons from C. elegans
Authors: Ma Yicong , Xie Jing , Wijaya Chandra Sugiarto , Xu Suhong
Published: October 2021

As a result of evolution, the ability to repair wounds allows organisms to combat environment insults. Although the general process of wound healing at the tissue level has been described for decades, the detailed molecular mechanisms regarding the early wound response and rapid wound repair at the cellular level remain little understood.Caenorhabditis elegansis a model organism widely used in the field of development, neuroscience, programmed cell death etc. The nematode skin is composed of a large epidermis associated with a transparent extracellular cuticle, which likely has a robust capacity for epidermal repair. Yet, until the last decades, relatively few studies had directly analyzed the wound response and repair process. Here we review recent findings in howC. elegansepidermis responds to wounding and initiates early actin-polymerization-based wound closure as well as later membrane repair. We also discussed some remained outstanding questions for future study.
Evolutionary insights into heart regeneration
Authors: Xiong Jing-Wei
Published: October 2021

Some lower vertebrates such as zebrafish and axolotl have incredible cardiac regenerative potential while mammals have very limited ones. Comparative studies among species have revealed that cardiomyocyte polyploidy, endothermy, and injury-induced activation of certain transcriptional factors including AP1 complexes are critical for cardiomyocyte proliferation and heart regeneration during animal evolution. Gaining insights into these evolutionarily conserved mechanisms will likely lead to achieving heart regeneration in non-regenerative mammals including humans.
RhoA/Rock activation represents a new mechanism for inactivating Wnt/β-catenin signaling in the aging-associated bone loss
Authors: Shi Wei , Xu Chengyun , Gong Ying , Wang Jirong , Ren Qianlei , Yan Ziyi , Mei Liu , Tang Chao , Ji Xing , Hu Xinhua , Qv Meiyu , Hussain Musaddique , Zeng Ling-Hui , Wu Ximei
Published: October 2021

The Wnt/β-catenin signaling pathway appears to be particularly important for bone homeostasis, whereas nuclear accumulation of β-catenin requires the activation of Rac1, a member of the Rho small GTPase family. The aim of the present study was to investigate the role of RhoA/Rho kinase (Rock)-mediated Wnt/β-catenin signaling in the regulation of aging-associated bone loss. We find that Lrp5/6-dependent and Lrp5/6-independent RhoA/Rock activation by Wnt3a activates Jak1/2 to directly phosphorylate Gsk3β at Tyr216, resulting in Gsk3β activation and subsequent β-catenin destabilization. In line with these molecular events, RhoA loss- or gain-of-function in mouse embryonic limb bud ectoderms interacts genetically with Dkk1 gain-of-function to rescue the severe limb truncation phenotypes or to phenocopy the deletion of β-catenin, respectively. Likewise, RhoA loss-of-function in pre-osteoblasts robustly increases bone formation while gain-of-function decreases it. Importantly, high RhoA/Rock activity closely correlates with Jak and Gsk3β activities but inversely correlates with β-catenin signaling activity in bone marrow mesenchymal stromal cells from elderly male humans and mice, whereas systemic inhibition of Rock therefore activates the β-catenin signaling to antagonize aging-associated bone loss. Taken together, these results identify RhoA/Rock-dependent Gsk3β activation and subsequent β-catenin destabilization as a hitherto uncharacterized mechanism controlling limb outgrowth and bone homeostasis.
miRNA in cardiac development and regeneration
Authors: Ouyang Zhaohui , Wei Ke
Published: October 2021

Ischemic heart disease is one of the main causes of morbidity and mortality in the world. In adult mammalian hearts, most cardiomyocytes are terminally differentiated and have extremely limited capacity of proliferation, making it impossible to regenerate the heart after injuries such as myocardial infarction. MicroRNAs (miRNAs), a class of non-coding single-stranded RNA, which are involved in mRNA silencing and the regulation of post-transcriptional gene expression, have been shown to play a crucial role in cardiac development and cardiomyocyte proliferation. Muscle specific miRNAs such as miR-1 are key regulators of cardiomyocyte maturation and growth, while miR-199-3p and other miRNAs display potent activity to induce proliferation of cardiomyocytes. Given their small size and relative pleiotropic effects, miRNAs have gained significant attraction as promising therapeutic targets or tools in cardiac regeneration. Increasing number of studies demonstrated that overexpression or inhibition of specific miRNAs could induce cardiomyocyte proliferation and cardiac regeneration. Some common targets of pro-proliferation miRNAs, such as the Hippo-Yap signaling pathway, were identified in multiple species, highlighting the power of miRNAs as probes to dissect core regulators of biological processes. A number of miRNAs have been shown to improve heart function after myocardial infarction in mice, and one trial in swine also demonstrated promising outcomes. However, technical difficulties, especially in delivery methods, and adverse effects, such as uncontrolled proliferation, remain. In this review, we summarize the recent progress in miRNA research in cardiac development and regeneration, examine the mechanisms of miRNA regulating cardiomyocyte proliferation, and discuss its potential as a new strategy for cardiac regeneration therapy.
Forkhead box family transcription factors as versatile regulators for cellular reprogramming to pluripotency
Authors: Fu Meijun , Chen Huan , Cai Zepo , Yang Yihang , Feng Ziyu , Zeng Mengying , Chen Lijun , Qin Yue , Cai Baomei , Zhu Pinghui , Zhou Chunhua , Yu Shengyong , Guo Jing , Liu Jing , Cao Shangtao , Pei Duanqing
Published: October 2021

Forkhead box (Fox) transcription factors play important roles in mammalian development and disease. However, their function in mouse somatic cell reprogramming remains unclear. Here, we report that FoxD subfamily and FoxG1 accelerate induced pluripotent stem cells (iPSCs) generation from mouse fibroblasts as early as day4 while FoxA and FoxO subfamily impede this process obviously. More importantly, FoxD3, FoxD4 and FoxG1 can replace Oct4 respectively and generate iPSCs with germline transmission together with Sox2 and Klf4. On the contrary, FoxO6 almost totally blocks reprogramming through inhibiting cell proliferation, suppressing the expression of pluripotent genes and hindering the process of mesenchymal to epithelial transition (MET). Thus, our study uncovers unexpected roles of Fox transcription factors in reprogramming and offers new insights into cell fate transition.
Scaffold strategies combined with mesenchymal stem cells in vaginal construction: a review
Authors: Henckes Nicole Andréa Corbellini , Faleiro Dalana , Chuang Laura Chao , Cirne-Lima Elizabeth Obino
Published: October 2021

Tissue engineering has provided new treatment alternatives for tissue reconstruction. Advances in the tissue engineering field have resulted in mechanical support and biological substitutes to restore, maintain or improve tissue/organs structures and functions. The application of tissue engineering technology in the vaginal reconstruction treatment can not only provide mechanical requirements, but also offer tissue repairing as an alternative to traditional approaches. In this review, we discuss recent advances in cell-based therapy in combination with scaffolds strategies that can potentially be adopted for gynaecological transplantation.
A hierarchical analysis of ecosystem classification with implementing in two continental ecoregions
Authors: Zhang YanQing
Published: October 2021

Background

The ecosystem classification of land (ECL) has been studied for a couple of decades, from the beginning of the perfect organism system “top-down” approach to a reversed “bottom-up” approach by defining a micro-ecological unit. After comparing two cases of the ecosystem classification framework implemented in the different continental ecoregions, the processes were carefully examined and justified.

Results

Theoretically, Bailey’s upper levels of ECL (Description of the ecoregions of the United States, 2nd ed. Rev and expanded (1st ed. 1980). Misc. Publ. No. 1391 (Rev). Washington DC USDA Forest Service; 1995) were applied to the United States and world continents. For the first time, a complete ECL study was accomplished in Western Utah of the United States, with eight upper levels of ECOMAP (National hierarchical framework of ecological units. U.S. Department of Agriculture, Forest Service, Washington, DC.https://www.researchgate.net/publication/237419014_National_hierarchical_framework_of_ecological_units; 1993) plus additional ecological site and vegetation stand. China’s Eco-geographic classification was most likely fitted into Bailey’s Ecosystem Classification upper-level regime. With a binary decision tree analysis, it had been validated that the Domains have an empty entity for 500 Plateau Domain between the US and China ecoregion framework. Implementing lower levels of ECL to Qinghai Province of China, based on the biogeoclimatic condition, vegetation distribution, landform, and plant species feature, it had classified the Section HIIC1 into two Subsections (labeled asi,ii), and delineatediiaof QiLian Mountain East Alpine Shrub and Alpine Tundra Ecozone intoiia-1andiia-2Subzones. Coordinately, an Ecological Site was completed at the bottom level.

Conclusions

(1) It was more experimental processing by implementing a full ECL in the Western Utah of the United States based on the ECOMAP (1993). (2) The empty entity, named as Plateau Domain 500, should be added into the top-level Bailey’s ecoregion framework. Coordinately, it includes the Divisions of HI and HII and the Provinces of humid, sub-humid, semiarid, and arid for China's Eco-Geographic region. (3) Implementing a full ECL in a different continent and integrating the lower level's models was the process that could handle the execution management, interpreting the relationship of ecosystem, dataset conversion, and error correction.
Exploring adults’ motives for food choice of sustainable diet components: a qualitative study in Tehran Metropolis
Authors: Haghighian Roudsari Arezoo , Vedadhir Abouali , Pourmoradian Samira , Rahimi-Ardabili Hania , Shokouhi Maryam , Milani-Bonab Ali
Published: October 2021

Background

Todays, due to the impact of human food choices on increasing greenhouse gas emissions, water consumption and environmental degradation, there is a new approach about changing the pattern of food production and consumption, including sustainable food and nutrition system related to consumption. This study aimed to explore the components of a sustainable diet among the factors that affect people’s food choices.

Methods

This qualitative study was carried out using an in-depth interview with 33 individuals aged 30–64 years old living in different areas of Tehran. Data collection, data analysis and theoretical conceptualization were performed simultaneously. MAXQDA 10 software was used for managing and organizing the data.

Results

In this paper, the findings are categorized according to the key components of a sustainable diet in five themes: “Health and Nutrition”, “Food and Agriculture Security”, “Environment and Ecosystems”, “Markets, food trade and production chains”, “social, cultural, and policy factors” were categorized. Meanwhile, the components of the “Health and Nutrition” domain had the highest contribution and the components of the two domains “food and agriculture” and “environment and ecosystems” had the lowest role based on the participants’ perception in this study.

Conclusion

Considering to the low importance of the components of a sustainable diet in food choices of the community, promoting the individual awareness of sustainable diet components, clarifying the importance of food choices in creating environmental impacts and leading the national macro policies in the field food and nutrition toward sustainable diet goals are essential.
Horror of three synergistic factors in THA: high mechanical stress, dissimilar metals, low elasticity stem: a case report
Authors: Ishii Seiya , Homma Yasuhiro , Matsukawa Takehisa , Baba Tomonori , Kubota Ayano , Yokoyama Kazuhito , Kaneko Kazuo , Ishijima Muneaki
Published: October 2021

Background

A large-diameter femoral head is effective in preventing dislocation after total hip arthroplasty. However, although rare, catastrophic stem tribocorrosion may occur at the head-stem junction.

Case presentation

A 70-year-old woman underwent revision surgery 7.5 years after total hip arthroplasty because of catastrophic stem corrosion with dissociation of the metal head (cobalt/chromium) and stem (TiMo12Zr6Fe2). Abnormal levels of cobalt were found in the intra-articular fluid, capsule, hip muscle, and blood. Revision surgery was performed via the direct anterior approach. The well-fixed femoral stem was explanted, and a cemented stainless stem with stainless head was implanted. Three months after the revision surgery, the cobalt concentration in the blood had decreased to normal.

Conclusions

Stem dissociation in the present case might have been caused by synergistic combination of a 36-mm-diameter femoral head and long neck length offset with high frictional torque, a cobalt-chromium head with a high risk of galvanic corrosion, and a TMZF (TiMo12Zr6Fe2) alloy stem with a low Young’s modulus of elasticity. The combination of these factors must be avoided.
Oxidative Stress and Inflammation Are Associated With Age-Related Endothelial Dysfunction in Men With Low Testosterone
Authors: Babcock Matthew C , DuBose Lyndsey E , Witten Teresa L , Stauffer Brian L , Hildreth Kerry L , Schwartz Robert S , Kohrt Wendy M , Moreau Kerrie L
Published: October 2021

Context

Vascular aging, including endothelial dysfunction secondary to oxidative stress and inflammation, increases the risk for age-associated cardiovascular disease (CVD). Low testosterone in middle-aged/older men is associated with increased CVD risk.

Objective

We hypothesized that low testosterone contributes to age-associated endothelial dysfunction, related in part to greater oxidative stress and inflammation.

Methods

This cross-sectional study included 58 healthy, nonsmoking men categorized as young (N = 20; age 29 ± 4 years; testosterone 500 ± 58 ng/dL), middle-aged/older with higher testosterone (N = 20; age 60 ± 6 years; testosterone 512 ± 115 ng/dL), and middle-aged/older lower testosterone (N = 18; age 59 ± 8 years; testosterone 269 ± 48 ng/dL). Brachial artery flow-mediated dilation (FMDBA) was measured during acute infusion of saline (control) and vitamin C (antioxidant). Markers of oxidative stress (total antioxidant status and oxidized low-density lipoprotein cholesterol), inflammation (interleukin [IL]-6 and C-reactive protein [CRP]), and androgen deficiency symptoms were also examined.

Results

During saline, FMDBA was reduced in middle-aged/older compared with young, regardless of testosterone status (P < 0.001). FMDBA was reduced in middle-aged/older lower testosterone (3.7% ± 2.0%) compared with middle-aged/older higher testosterone (5.7% ± 2.2%; P = 0.021), independent of symptoms. Vitamin C increased FMDBA (to 5.3% ± 1.6%; P = 0.022) in middle-aged/older lower testosterone but had no effect in young (P = 0.992) or middle-aged/older higher testosterone (P = 0.250). FMDBA correlated with serum testosterone (r = 0.45; P < 0.001), IL-6 (r = −0.41; P = 0.002), and CRP (r = −0.28; P = 0.041).

Conclusion

Healthy middle-aged/older men with low testosterone appear to have greater age-associated endothelial dysfunction, related in part to greater oxidative stress and inflammation. These data suggest that low testosterone concentrations may contribute to accelerated vascular aging in men.
Detrimental or beneficial? Untangling the literature on developmental stress studies in birds
Authors: Wada Haruka , Coutts Victoria
Published: October 2021

Developing animals display a tremendous ability to change the course of their developmental path in response to the environment they experience, a concept referred to as developmental plasticity. This change in behavior, physiology or cellular processes is primarily thought to allow animals to better accommodate themselves to the surrounding environment. However, existing data on developmental stress and whether it brings about beneficial or detrimental outcomes show conflicting results. There are several well-referred hypotheses related to developmental stress in the current literature, such as the environmental matching, silver spoon and thrifty phenotype hypotheses. These hypotheses speculate that the early-life environment defines the capacity of the physiological functions and behavioral tendencies and that this change is permanent and impacts the fitness of the individual. These hypotheses also postulate there is a trade-off among organ systems and physiological functions when resources are insufficient. Published data on avian taxa show that some effects of developmental nutritional and thermal stressors are long lasting, such as the effects on body mass and birdsong. Although hypotheses on developmental stress are based on fitness components, data on reproduction and survival are scarce, making it difficult to determine which hypothesis these data support. Furthermore, most physiological and performance measures are collected only once; thus, the physiological mechanisms remain undertested. Here, we offer potential avenues of research to identify reasons behind the contrasting results in developmental stress research and possible ways to determine whether developmental programming due to stressors is beneficial or detrimental, including quantifying reproduction and survival in multiple environments, measuring temporal changes in physiological variables and testing for stress resistance later in life.
Tadpole-transporting frogs use stagnant water odor to find pools in the rainforest
Authors: Serrano-Rojas Shirley J. , Pašukonis Andrius
Published: October 2021

Breeding sites are often a limited and ephemeral resource for rainforest frogs. This resource limitation has driven the evolution of diverse reproductive strategies that increase offspring survival. For example, poison frogs shuttle their tadpoles from terrestrial clutches to aquatic rearing sites, using various cues to assess pool suitability. Yet, how frogs find new pools is unknown. We tested the role of odor cues in the process of finding tadpole deposition sites by the poison frog Allobates femoralis. We created 60 artificial pools grouped into three conditions: stagnant water, tadpole water and clean water control. Fifteen pools were discovered within 6 days, with more tadpoles and more frogs directly observed at pools with stagnant odor cues. Our findings suggest that frogs use odor cues associated with stagnant water for the initial discovery of new breeding pools. These cues may be good indicators of pool stability and increased likelihood of tadpole survival.
Proteomic Investigation in Plasma from Women with Fibromyalgia in Response to a 15-wk Resistance Exercise Intervention
Authors: WÅHLÉN KARIN , YAN HONG , WELINDER CHARLOTTE , ERNBERG MALIN , KOSEK EVA , MANNERKORPI KAISA , GERDLE BJÖRN , GHAFOURI BIJAR
Published: October 2021

Purpose

Fibromyalgia (FM) is a complex pain condition, and exercise is considered the first option of treatment. Few studies have examined the effect of exercise on molecular mechanisms in FM. The aim of this study was to analyze the plasma proteome in women with FM and healthy controls (CON) before and after 15 wk of resistance exercise. This study further investigated whether clinical and exercises-related outcomes correlated with identified plasma proteins in FM.

Methods

Plasma samples from 40 FM/25 CON (baseline) and 21 FM/24 CON (postexercise) were analyzed using shotgun proteomics. Clinical/background data were retrieved through questionnaires. Exercise-related variables and pressure pain thresholds were assessed using standardized instruments. Multivariate statistics were applied to analyze the proteomic profile at baseline and postexercise, and correlation with clinical/exercise-related data.

Results

Fifteen weeks of resistance exercises improved clinical symptoms and muscle strength, and affected circulating proteins related to immunity, stress, mRNA stability, metabolic processes, and muscle structure development in FM. Pressure pain threshold was related to a specific protein profile, with proteins involved in metabolic and immune response. Subgroups of FM based on plasma proteins, FM duration, and improved muscle strength were identified.

Conclusions

Exercise seems to affect circulating proteins, clinical characteristics, and muscle strength in FM. This study contributes to better understanding of systemic protein changes in FM compared with CON and how resistance exercise affects such changes.
Mild Cognitive Impairment and Dementia Reported by Former Professional Football Players over 50 yr of Age: An NFL-LONG Study
Authors: WALTON SAMUEL R. , BRETT BENJAMIN L. , CHANDRAN AVINASH , DEFREESE J. D. , MANNIX REBEKAH , ECHEMENDIA RUBEN J. , MEEHAN WILLIAM P. , MCCREA MICHAEL , GUSKIEWICZ KEVIN M. , KERR ZACHARY Y.
Published: October 2021

Purpose

This study aimed to estimate prevalence of mild cognitive impairment (MCI) and dementia diagnoses in former National Football League (NFL) players ≥50 yr old and examine the relationships among these diagnoses and an array of predictors of long-term brain health.

Methods

A cross section of former NFL players (n = 922; mean ± SD age, 64.8 ± 8.9 yr) completed a questionnaire. Prevalence of self-reported medical diagnoses of MCI and dementia was reported alongside U.S. population estimates across 5-yr age intervals (e.g., 60–64 yr). Prevalence ratios (PR) were calculated for multiple predictors of long-term brain health.

Results

Overall, MCI prevalence and dementia prevalence were n = 219(23.8%) and n = 82(8.9%), respectively. Each diagnosis was more prevalent in former NFL players across age-groups than U.S. norms, with greater disparities at relatively younger ages (e.g., 65–69 yr) compared with older ages. Greater prevalence of MCI and dementia was associated with self-reported concussion history (10+ vs 0; PRadjusted [95% CI] = 1.66 [1.02–2.71] and 2.61 [1.01–6.71], respectively); recent pain intensity (PRadjusted [95% CI] = 1.13 [1.07–1.20] and 1.15 [1.03–1.28]); and diagnoses of depression (PRadjusted [95% CI] = 2.70 [1.92–3.81] and 3.22 [1.69–6.14]), anxiety (PRadjusted [95% CI] = 1.96 [1.26–3.07] and 3.14 [1.47–6.74]), or both (PRadjusted [95% CI] = 3.11 [2.38–4.08] and 4.43 [2.71–7.25]). Higher MCI prevalence was related to sleep apnea (PRadjusted [95% CI] = 1.30 [1.06–1.60]); higher dementia prevalence was associated with age (5-yr interval, PRadjusted [95% CI] = 1.42 [1.26–1.60]) and race (non-White vs White, PRadjusted [95% CI] = 1.64 [1.07–2.53]).

Conclusions

Self-reported MCI prevalence and dementia prevalence were higher in former NFL players than national estimates and were associated with numerous personal factors, including mood-related disorders and a high number of self-reported concussions. Predictors of higher MCI and dementia prevalence may be modifiable and warrant consideration by clinicians and researchers as potential targets to mitigate the onset of these conditions.
DarkQ: continuous genomic monitoring using message queues
Authors: Viehweger Adrian , Brandt Christian , Hölzer Martin
Published: October 2021

Newly sequenced genomes are often not noticed by potential stakeholders because submission to public databases is delayed, and search options are limited. However, the discovery of genomes can be vital: in pathogen outbreaks, fast updates are essential to coordinate containment efforts and prevent further spread. Here we introduce DarkQ, a message queue that allows for instant sharing and discovery of genomes.

DarkQ is released under the BSD-2 license at github.com/phiweger/darkq.