bluuebunny/crossref_metadata_2025 huggingface dataset:
WALL STABILIZATION IN MINES BY SPRAY-ON LINERS
Authors: MASON D. P. , FOWKES N. D. , YEMATA R. M. , ONYEAGOZIRI C. A , YILMAZ H.
Published: August 2023

Thin spray-on liners (TSLs) have been found to be effective for structurally supporting the walls of mining tunnels and thus reducing the occurrence of rock bursts, an effect primarily due to the penetration of cracks by the liner. Surface tension effects are thus important. However, TSLs are also used to simply stabilize rock surfaces, for example, to prevent rock fall, and in this context crack penetration is desirable but not necessary, and the tensile and shearing strength and adhesive properties of the liner determine its effectiveness. We examine the effectiveness of nonpenetrating TSLs in a global lined tunnel and in a local rock support context. In the tunnel context, we examine the effect of the liner on the stress distribution in a tunnel subjected to a geological or mining event. We show that the liner has little effect on stresses in the surrounding rock and that tensile stresses in the rock surface are transmitted across the liner, so that failure is likely to be due to liner rupture or detachment from the surface. In the local rock support context, loose rock movements are shown to be better achieved using a liner with small Young’s modulus, but high rupture strength.
At the frontlines of implementing the right to a healthy environment: Understanding human rights and environmental due diligence in relation to armed conflicts
Authors: Kron Amanda
Published: August 2023

Potential harm to human rights and the environment, including by corporate actors, is amplified in situations of conflict. This article focuses on applying the right to a healthy environment in relation to armed conflicts and corporate responsibility. In particular, it analyzes and compares due diligence requirements in the European Union Conflict Minerals Regulation and the International Law Commission's Draft Principles on Protection of the Environment in Relation to Armed Conflicts and examines how these align with the right to a healthy environment.
The Great Climate Mitigation Potential of Cropland Ecosystem Management in China
Authors: Zheng Huan , Guo Lanlan , He Bin , Shen Yanjun , Yuan Wenping , Yang Wentao , Li Tiewei , Zheng Hao
Published: August 2023

Limiting climate warming to below 2°C requires more intense mitigation efforts. Alongside the steep reductions in fossil fuel emissions needed, natural climate solutions (NCS) act as an additional first step toward achieving China's emission reduction goals. We first quantified the historical contribution of NCS as 0.34 PgCO2equivalent (PgCO2e) yr−1through the implemented national ecological restoration projects as well as positive management measures. Based on successful practices, we then designed 21 pathways to quantify the potential of NCS in increasing terrestrial carbon sinks or reducing greenhouse gas (GHG) emissions in forest, grassland, cropland, and wetland ecosystems. We show that the maximum mitigation potential of NCS between 2020 and 2060 under guaranteed food and fiber security is 1.95 (1.54–2.38) PgCO2e yr−1(95% CI), which can offset nearly 20% of the annual net emissions. Historically, forest ecosystems have contributed the most to carbon sequestration; however, future projections show cropland ecosystems will account for the largest proportion (47.6%), followed by forest ecosystems. Approximately 47% (0.91 PgCO2e yr−1) of the maximum represents cost‐effective mitigation potential that can be realized at ≤100 USD/MgCO2e. Conservation tillage is the most cost‐effective option in China, achieving a maximum contribution of 181.60 TgCO2e yr−1. In addition, a series of cropland management measures have contributed 1.14 PgCO2e in the past two decades. Our study highlights the high effectiveness of cropland pathways in reducing carbon emissions and emphasizes the key role of cropland ecosystems in mitigating climate change.
The Largest Ever Recorded Heatwave—Characteristics and Attribution of the Antarctic Heatwave of March 2022
Authors: Blanchard‐Wrigglesworth Edward , Cox Tyler , Espinosa Zachary I. , Donohoe Aaron
Published: August 2023

An unprecedented heatwave impacted East Antarctica in March 2022, peaking at 39°C above climatology, the largest temperature anomaly ever recorded globally. We investigate the causes of the heatwave, the impact of climate change, and a climate model's ability in simulating such an event. The heatwave, which was skillfully forecast, resulted from a highly anomalous large‐scale circulation pattern that advected an Australian airmass to East Antarctica in 4 days and produced record atmospheric heat fluxes. Southern Ocean sea surface temperatures anomalies had a minimal impact on the heatwave's amplitude. Simulations from a climate model fail to simulate such a large temperature anomaly mostly due to biases in its large‐scale circulation variability, showcasing a pathway for future model improvement in simulating extreme heatwaves. The heatwave was made 2°C warmer by climate change, and end of 21st century heatwaves may be an additional 5–6°C warmer, raising the prospect of near‐melting temperatures over the interior of East Antarctica.
The 6 February 2023 Türkiye Earthquake Sequence as Detected in the Ionosphere
Authors: Maletckii B. , Astafyeva E. , Sanchez S. A. , Kherani E. A. , de Paula E. R.
Published: August 2023

On 6 February 2023, a series of large earthquakes struck Turkey and Northern Syria. The main earthquake of Mw 7.8 occurred at 01:17:34 UTC and was followed by the three notable (Mw > 5.5) aftershocks within the next 18 min. Then, ∼9 hr later, the biggest aftershock with magnitude Mw 7.5 and a Mw 6.0 earthquake occurred to the north‐east from the first main earthquake. In this work, we use data of ground‐based Global Navigation Satellite Systems (GNSS) receivers in Turkey, Israel and Cyprus to analyze the ionospheric response to this series of earthquakes. We separate these events in two groups: the first sequence of earthquakes (at 01–02 UTC) and the second sequence (at 10–11 UTC). For the first sequence, we observe a clear N‐shaped total electron content (TEC) response after the Mw 7.8 mainshock earthquake and Mw 6.7 aftershock, and a smaller TEC disturbance that is, most likely, caused by the Mw 5.6 earthquake. The latter is now the smallest earthquake detected by using ionospheric GNSS data. The co‐seismic ionospheric disturbances (CSID) propagated from the epicentral area in the south‐west direction with velocities of about 750–830 m/s. For the second sequence, we observed the response to the Mw 7.5 aftershock earthquake and the Mw 6.0 aftershock. The CSID propagated both to the south‐west and the north‐west to the epicentral area, with velocities of about 950–1,100 m/s.
Quantifying Site Effects and Their Influence on Earthquake Source Parameter Estimations Using a Dense Array in Oklahoma
Authors: Chang Hilary , Abercrombie Rachel E. , Nakata Nori , Pennington Colin N. , Kemna Kilian B. , Cochran Elizabeth S. , Harrington Rebecca M.
Published: August 2023

We investigate the effects of site response on source parameter estimates using earthquakes recorded by the LArge‐n Seismic Survey in Oklahoma (LASSO). While it is well known that near‐surface unconsolidated sediments can cause an apparent breakdown of earthquake self‐similarity, the influence of laterally varying site conditions remains unclear. We analyze site conditions across the 1825‐station array on a river plain within an area of 40 km by 23 km using vertical ground motions from 14 regional earthquakes. While the source radiation pattern controls P‐wave ground motions below 8 Hz, the surface geology correlates with P‐wave ground motions above 8 Hz and S‐wave ground motions at 2–21 Hz. Stations installed in alluvial sediments have vertical ground motions that can exceed three times the array median. We use the variation of ground motion of regional earthquakes across the array as a proxy for site effects. The corner frequencies and stress drops of local earthquakes (ML = 0.01–3) estimated using a standard single‐spectra approach show negative correlations with the site‐effect proxy, while the seismic moments show positive correlations. In contrast, the spectral‐ratio approach effectively shows no correlation. The overall bias is small as expected for this relatively homogeneous structure; accurate estimation of site‐related biases requires at least 30 stations. Correcting for site‐related biases reduces the standard deviations of the source parameters by less than 13% of the total variations. Remaining variations are partially associated with source directivity and model misfits— as small earthquakes can have complex ruptures.
Rethinking Variability in Bedrock Rivers: Sensitivity of Hillslope Sediment Supply to Precipitation Events Modulates Bedrock Incision During Floods
Authors: DeLisle Clarke , Yanites Brian J.
Published: August 2023

Bedrock rivers are the pacesetters of landscape evolution in uplifting fluvial landscapes. Water discharge variability and sediment transport are important factors influencing bedrock river processes. However, little work has focused on the sensitivity of hillslope sediment supply to precipitation events and its implications on river evolution in tectonically active landscapes. We model the temporal variability of water discharge and the sensitivity of sediment supply to precipitation events as rivers evolve to equilibrium over 106 model years. We explore how coupling sediment supply sensitivity with discharge variability influences rates and timing of river incision across climate regimes. We find that sediment supply sensitivity strongly impacts which water discharge events are the most important in driving river incision and modulates channel morphology. High sediment supply sensitivity focuses sediment delivery into the largest river discharge events, decreasing rates of bedrock incision during floods by orders of magnitude as rivers are inundated with new sediment that buries bedrock. The results show that the use of river incision models in which incision rates increase monotonically with increasing river discharge may not accurately capture bedrock river dynamics in all landscapes, particularly in steep landslide prone landscapes. From our modeling results, we hypothesize the presence of an upper discharge threshold for river incision at which storms transition from being incisional to depositional. Our work illustrates that sediment supply sensitivity must be accounted for to predict river evolution in dynamic landscapes. Our results have important implications for interpreting and predicting climatic and tectonic controls on landscape morphology and evolution.
Advancing optothermal manipulation: decoupling temperature and flow fields in quasi-isothermal microscale streaming
Authors: Kim Youngsun , Zheng Yuebing
Published: August 2023

By decoupling temperature and flow fields through symmetry-correlated laser scan sequences, ISO-FLUCS enables quasi-isothermal optofluidic microscale streaming. This technique offers precise control over fluid manipulation while minimizing thermal damage.
Infrared avalanche photodiodes from bulk to 2D materials
Authors: Martyniuk Piotr , Wang Peng , Rogalski Antoni , Gu Yue , Jiang Ruiqi , Wang Fang , Hu Weida
Published: August 2023

Avalanche photodiodes (APDs) have drawn huge interest in recent years and have been extensively used in a range of fields including the most important one—optical communication systems due to their time responses and high sensitivities. This article shows the evolution and the recent development of AIIIBV, AIIBVI, and potential alternatives to formerly mentioned—“third wave” superlattices (SL) and two-dimensional (2D) materials infrared (IR) APDs. In the beginning, the APDs fundamental operating principle is demonstrated together with progress in architecture. It is shown that the APDs evolution has moved the device’s performance towards higher bandwidths, lower noise, and higher gain-bandwidth products. The material properties to reach both high gain and low excess noise for devices operating in different wavelength ranges were also considered showing the future progress and the research direction. More attention was paid to advances in AIIIBVAPDs, such as AlInAsSb, which may be used in future optical communications, type-II superlattice (T2SLs, “Ga-based” and “Ga-free”), and 2D materials-based IR APDs. The latter—atomically thin 2D materials exhibit huge potential in APDs and could be considered as an alternative material to the well-known, sophisticated, and developed AIIIBVAPD technologies to include single-photon detection mode. That is related to the fact that conventional bulk materials APDs’ performance is restricted by reasonably high dark currents. One approach to resolve that problem seems to be implementing low-dimensional materials and structures as the APDs’ active regions. The Schottky barrier and atomic level thicknesses lead to the 2D APD dark current significant suppression. What is more, APDs can operate within visible (VIS), near-infrared (NIR)/mid-wavelength infrared range (MWIR), with a responsivity ~80 A/W, external quantum efficiency ~24.8%, gain ~105for MWIR [wavelength,λ = 4 μm, temperature,T = 10–180 K, Black Phosphorous (BP)/InSe APD]. It is believed that the 2D APD could prove themselves to be an alternative providing a viable method for device fabrication with simultaneous high-performance—sensitivity and low excess noise.
Chaos Raman distributed optical fiber sensing
Authors: Wang Chenyi , Li Jian , Zhou Xinxin , Cheng Zijia , Qiao Lijun , Xue Xiaohui , Zhang Mingjiang
Published: August 2023

The physics principle of pulse flight positioning is the main theoretical bottleneck that restricts the spatial resolution of the existing Raman distributed optical fiber sensing scheme. Owing to the pulse width of tens of nanoseconds, the spatial resolution of the existing Raman distributed optical fiber sensing scheme with kilometer-level sensing distance is limited to the meter level, which seriously restricts the development of the optical time-domain reflection system. In this paper, a chaos laser is proposed in the context of the physical principle of the Raman scattering effect, and a novel theory of chaos Raman distributed optical fiber sensing scheme is presented. The scheme reveals the characteristics of chaos Raman scattering light excited by a chaotic signal on the sensing fiber. Further, the chaos time-domain compression demodulation mechanism between the temperature variation information and chaos correlation peak is demonstrated. Then, the position of the temperature variation signal is precisely located using the delay time of the chaos correlation peak combined with the chaos pulse flight time. Based on this novel optical sensing mechanism, an experiment with 10 cm spatial resolution and 1.4 km sensing distance was conducted, and the spatial resolution was found to be independent of the sensing distance. Within the limit of the existing spatial resolution theory, the spatial resolution of the proposed scheme is 50 times higher than that of the traditional scheme. The scheme also provides a new research direction for optical chaos and optical fiber sensing.
Emergence of putative energy parasites within Clostridia revealed by genome analysis of a novel endosymbiotic clade
Authors: Takahashi Kazuki , Kuwahara Hirokazu , Horikawa Yutaro , Izawa Kazuki , Kato Daiki , Inagaki Tatsuya , Yuki Masahiro , Ohkuma Moriya , Hongoh Yuichi
Published: August 2023

The Clostridia is a dominant bacterial class in the guts of various animals and are considered to nutritionally contribute to the animal host. Here, we discovered clostridial endosymbionts of cellulolytic protists in termite guts, which have never been reported with evidence. We obtained (near-)complete genome sequences of three endosymbiotic Clostridia, each associated with a different parabasalid protist species with various infection rates: Trichonympha agilis, Pseudotrichonympha grassii, and Devescovina sp. All these protists are previously known to harbor permanently-associated, mutualistic Endomicrobia or Bacteroidales that supplement nitrogenous compounds. The genomes of the endosymbiotic Clostridia were small in size (1.0–1.3 Mbp) and exhibited signatures of an obligately-intracellular parasite, such as an extremely limited capability to synthesize amino acids, cofactors, and nucleotides and a disrupted glycolytic pathway with no known net ATP-generating system. Instead, the genomes encoded ATP/ADP translocase and, interestingly, regulatory proteins that are unique to eukaryotes in general and are possibly used to interfere with host cellular processes. These three genomes formed a clade with metagenome-assembled genomes (MAGs) derived from the guts of other animals, including human and ruminants, and the MAGs shared the characteristics of parasites. Gene flux analysis suggested that the acquisition of the ATP/ADP translocase gene in a common ancestor was probably key to the emergence of this parasitic clade. Taken together, we provide novel insights into the multilayered symbiotic system in the termite gut by adding the presence of parasitism and present an example of the emergence of putative energy parasites from a dominant gut bacterial clade.
A route to metalloligands consolidated silver nanoclusters by grafting thiacalix[4]arene onto polyoxovanadates
Authors: Wang Zhi , Zhu Yan-Jie , Han Bao-Liang , Li Yi-Zhi , Tung Chen-Ho , Sun Di
Published: August 2023

Metalloligands provide a potent strategy for manipulating the surface metal arrangements of metal nanoclusters, but their synthesis and subsequent installation onto metal nanoclusters remains a significant challenge. Herein, two atomically precise silver nanoclusters {Ag14[(TC4A)6(V9O16)](CyS)3} (Ag14) and {Ag43S[(TC4A)2(V4O9)]3(CyS)9(PhCOO)3Cl3(SO4)4(DMF)3·6DMF} (Ag43) are synthesized by controlling reaction temperature (H4TC4A = p-tert-butylthiacalix[4]arene). Interestingly, the 3D scaffold-like [(TC4A)6(V9O16)]11- metalloligand in Ag14 and 1D arcuate [(TC4A)2(V4O9)]6- metalloligand in Ag43 exhibit a dual role that is the internal polyoxovanadates as anion template and the surface TC4A4- as the passivating agent. Furthermore, the thermal-induced structure transformation between Ag14 and Ag43 is achieved based on the temperature-dependent assembly process. Ag14 shows superior photothermal conversion performance than Ag43 in solid state indicating its potential for remote laser ignition. Here, we show the potential of two thiacalix[4]arene modified polyoxovanadates metalloligands in the assembly of metal nanoclusters and provide a cornerstone for the remote laser ignition applications of silver nanoclusters.
Synthesize high-dimensional longitudinal electronic health records via hierarchical autoregressive language model
Authors: Theodorou Brandon , Xiao Cao , Sun Jimeng
Published: August 2023

Synthetic electronic health records (EHRs) that are both realistic and privacy-preserving offer alternatives to real EHRs for machine learning (ML) and statistical analysis. However, generating high-fidelity EHR data in its original, high-dimensional form poses challenges for existing methods. We propose Hierarchical Autoregressive Language mOdel () for generating longitudinal, high-dimensional EHR, which preserve the statistical properties of real EHRs and can train accurate ML models without privacy concerns. generates a probability density function over medical codes, clinical visits, and patient records, allowing for generating realistic EHR data without requiring variable selection or aggregation. Extensive experiments demonstrated that can generate high-fidelity data with high-dimensional disease code probabilities closely mirroring (above 0.9 R2 correlation) real EHR data. also enhances the accuracy of predictive modeling and enables downstream ML models to attain similar accuracy as models trained on genuine data.
Antibody-mediated neutralization of galectin-3 as a strategy for the treatment of systemic sclerosis
Authors: Ortega-Ferreira Céline , Soret Perrine , Robin Gautier , Speca Silvia , Hubert Sandra , Le Gall Marianne , Desvaux Emiko , Jendoubi Manel , Saint-Paul Julie , Chadli Loubna , Chomel Agnès , Berger Sylvie , Nony Emmanuel , Neau Béatrice , Fould Benjamin , Licznar Anne , Levasseur Franck , Guerrier Thomas , Elouej Sahar , Courtade-Gaïani Sophie , Provost Nicolas , Nguyen The Quyen , Verdier Julien , Launay David , De Ceuninck Frédéric
Published: August 2023

Systemic sclerosis (SSc) is an autoimmune, inflammatory and fibrotic disease with limited treatment options. Developing new therapies is therefore crucial to address patient needs. To this end, we focused on galectin-3 (Gal-3), a lectin known to be associated with several pathological processes seen in SSc. Using RNA sequencing of whole-blood samples in a cross-sectional cohort of 249 patients with SSc, Gal-3 and its interactants defined a strong transcriptomic fingerprint associated with disease severity, pulmonary and cardiac malfunctions, neutrophilia and lymphopenia. We developed new Gal-3 neutralizing monoclonal antibodies (mAb), which were then evaluated in a mouse model of hypochlorous acid (HOCl)-induced SSc. We show that two of these antibodies, D11 and E07, reduced pathological skin thickening, lung and skin collagen deposition, pulmonary macrophage content, and plasma interleukin-5 and -6 levels. Moreover, E07 changed the transcriptional profiles of HOCl-treated mice, resulting in a gene expression pattern that resembled that of control mice. Similarly, pathological pathways engaged in patients with SSc were counteracted by E07 in mice. Collectively, these findings demonstrate the translational potential of Gal-3 blockade as a therapeutic option for SSc.
Coronary heart disease prediction method fusing domain-adaptive transfer learning with graph convolutional networks (GCN)
Authors: Lin Huizhong , Chen Kaizhi , Xue Yutao , Zhong Shangping , Chen Lianglong , Ye Mingfang
Published: August 2023

Graph convolutional networks (GCNs) have achieved impressive results in many medical scenarios involving graph node classification tasks. However, there are difficulties in transfer learning for graph representation learning and graph network models. Most GNNs work only in a single domain and cannot transfer the learned knowledge to other domains. Coronary Heart Disease (CHD) is a high-mortality disease, and there are non-public and significant differences in CHD datasets for current research, which makes it difficult to perform unified transfer learning. Therefore, in this paper, we propose a novel adversarial domain-adaptive multichannel graph convolutional network (DAMGCN) that can perform graph transfer learning on cross-domain tasks to achieve cross-domain medical knowledge transfer on different CHD datasets. First, we use a two-channel GCN model for feature aggregation using local consistency and global consistency. Then, a uniform node representation is generated for different graphs using an attention mechanism. Finally, we provide a domain adversarial module to decrease the discrepancies between the source and target domain classifiers and optimize the three loss functions in order to accomplish source and target domain knowledge transfer. The experimental findings demonstrate that our model performs best on three CHD datasets, and its performance is greatly enhanced by graph transfer learning.
Impact of vertical stratification on the 2020 spring bloom in the Yellow Sea
Authors: Kim Go-Un , Lee Jaeik , Kim Yong Sun , Noh Jae Hoon , Kwon Young Shin , Lee Howon , Lee Meehye , Jeong Jongmin , Hyun Myung Jin , Won Jongseok , Jeong Jin-Yong
Published: August 2023

The Yellow Sea is one of the world’s most abundant marine resources, providing food and economic benefits to the Korean and Chinese populations. In spring 2020, a decrease in the intensity of phytoplankton bloom was observed. While one study attributed this decline to a decrease in nutrient associated with the COVID-19 pandemic, our previous research proposed weakened thermal stratification accompanied by a surface cooling anomaly as the cause. However, the relationship between the marine environment and ecosystem has not been fully elucidated. Using observations and marine physical-biogeochemical model data, we identified the weakened stratification as a critical factor for suppressing the 2020 spring bloom. Intense vertical mixing hindered the accumulation of nutrient and chlorophyll-a concentrations within the euphotic zone, resulting in a diminished phytoplankton bloom. In contrast, reduced nitrate and phosphate concentrations in 2020 were insignificant compared to those in 2017–2019, despite the notable decline in PM2.5 in March 2020 due to COVID-19. In April 2020, nutrient levels fell within the range of interannual variability based on long-term observations, reflecting a negligible effect on the spring phytoplankton bloom. Our findings provide insight into the importance of marine physical factors on the phytoplankton biomass in the Yellow Sea.
Individual characteristics moderate listening effort in noisy classrooms
Authors: Visentin Chiara , Pellegatti Matteo , Garraffa Maria , Di Domenico Alberto , Prodi Nicola
Published: August 2023

Comprehending the teacher’s message when other students are chatting is challenging. Even though the sound environment is the same for a whole class, differences in individual performance can be observed, which might depend on a variety of personal factors and their specific interaction with the listening condition. This study was designed to explore the role of individual characteristics (reading comprehension, inhibitory control, noise sensitivity) when primary school children perform a listening comprehension task in the presence of a two-talker masker. The results indicated that this type of noise impairs children’s accuracy, effort, and motivation during the task. Its specific impact depended on the level and was modulated by the child’s characteristics. In particular, reading comprehension was found to support task accuracy, whereas inhibitory control moderated the effect of listening condition on the two measures of listening effort included in the study (response time and self-ratings), even though with a different pattern of association. A moderation effect of noise sensitivity on perceived listening effort was also observed. Understanding the relationship between individual characteristics and classroom sound environment has practical implications for the acoustic design of spaces promoting students’ well-being, and supporting their learning performance.
Housing situations and local COVID-19 infection dynamics using small-area data
Authors: Freise Diana , Schiele Valentin , Schmitz Hendrik
Published: August 2023

Low socio-economic status is associated with higher SARS-CoV-2 incidences. In this paper we study whether this is a result of differences in (1) the frequency, (2) intensity, and/or (3) duration of local SARS-CoV-2 outbreaks depending on the local housing situations. So far, there is not clear evidence which of the three factors dominates. Using small-scale data from neighborhoods in the German city Essen and a flexible estimation approach which does not require prior knowledge about specific transmission characteristics of SARS-CoV-2, behavioral responses or other potential model parameters, we find evidence for the last of the three hypotheses. Outbreaks do not happen more often in less well-off areas or are more severe (in terms of the number of cases), but they last longer. This indicates that the socio-economic gradient in infection levels is at least in parts a result of a more sustained spread of infections in neighborhoods with worse housing conditions after local outbreaks and suggests that in case of an epidemic allocating scarce resources in containment measures to areas with poor housing conditions might have the greatest benefit.
Etiology and clinical characteristics of acute viral hepatitis in South Korea during 2020–2021: a prospective multicenter study
Authors: Jeong Chan Young , Choi Gwang Hyeon , Jang Eun Sun , Kim Young Seok , Lee Youn-Jae , Kim In Hee , Cho Sung Bum , Yoon Jae Hyun , Kim Kyung-Ah , Choi Dae Hee , Chung Woo Jin , Cho Hyun Chin , Na Seong Kyun , Kim Yun-Tae , Lee Byung Seok , Jeong Sook-Hyang
Published: August 2023

This prospective, 12-center study investigated the etiology and clinical characteristics of acute viral hepatitis (AVH) during 2020–2021 in South Korea, and the performance of different diagnostic methods for hepatitis E virus (HEV). We enrolled 428 patients with acute hepatitis, of whom 160 (37.4%) were diagnosed with AVH according to predefined serologic criteria. The clinical data and risk factors for AVH were analyzed. For hepatitis E patients, anti-HEV IgM and IgG were tested with two commercial ELISA kits (Abia and Wantai) with HEV-RNA real-time RT-PCR. HAV, HEV, HBV, HCV, Epstein-Barr virus (EBV), cytomegalovirus, and herpes simplex virus accounted for AVH in 78.8% (n = 126), 7.5% (n = 12), 3.1% (n = 5), 1.9% (n = 3), 6.9% (n = 11), 1.2% (n = 2), and 0.6% (n = 1) of 160 patients (median age, 43 years; men, 52.5%; median ALT, 2144 IU/L), respectively. Hospitalization, hemodialysis, and intensive care unit admission were required in 137 (86.7%), 5 (3.2%), and 1 (0.6%) patient, respectively. Two patients developed acute liver failure (1.3%), albeit without mortality or liver transplantation. Ingestion of uncooked clams/oysters and wild boars’ blood/bile was reported in 40.5% and 16.7% of patients with HAV and HEV, respectively. The concordance rate between the anti-HEV-IgM results of both ELISA kits was 50%. HEV RNA was detected in only 17% of patients with HEV. The diagnosis of HEV needs clinical consideration due to incomplete HEV diagnostics.
Purification and fractionation of phycobiliproteins from Arthrospira platensis and Corallina officinalis with evaluating their biological activities
Authors: Ismail Mona M. , El-Fakharany Esmail M. , Hegazy Ghada E.
Published: August 2023

Phycobiliproteins (PBPs) are a class of water-soluble pigments with a variety of biological functions that are present in red macroalgae and cyanobacterial species. The crude forms of phycocyanin (C-PC) from the blue green alga Arthrospira platensis and allophycocyanin (APC) from the red macroalga Corallina officinalis were extracted and purified by ammonium sulphate precipitation, anion exchange chromatography, and size exclusion chromatography methods, respectively. The obtained C-PC and APC from A. platensis and C. officinalis were 0.31 mg/mL and 0.08 mg/mL, respectively, with molecular masses of “17.0 KDa and 19.0 KDa” and “15.0 KDa and 17.0 KDa” corresponding to α and β subunits, respectively. FT-IR was used to characterize the purified APC and C-PC in order to look into their structures. Highly purified extracts (A620/A280 > 4.0) were obtained from subtractions’ PC3 and PC4 that were tested for their biological activities. APC and C-PC crude extracts plus their fractions exhibited potent anti-oxidant in different ratios by using three techniques. PC1 showed high anti-inflammatory (75.99 and 74.55%) and anti-arthritic (78.89 and 76.92%) activities for C. officinalis and A. platensis, respectively compared with standard drugs (72.02 and 71.5%). The methanolic and water extracts of both species showed greater antibacterial efficacy against Gram +ve than Gram −ve marine bacteria. Our study shed light on the potential medical uses of C-PC and APC extracted from the tested species as natural substances in a variety of foods and drugs. Further investigations are required to explore the diverse chemical natures of distinct PBPs from different cyanobacteria and red algae because their amino acid sequences vary among different algal species.
Comparative and phylogenetic analysis of complete chloroplast genome sequences of Salvia regarding its worldwide distribution
Authors: Yu Dade , Pei Yifei , Cui Ning , Zhao Guiping , Hou Mengmeng , Chen Yingying , Chen Jialei , Li Xiwen
Published: August 2023

Salvia is widely used as medicine, food, and ornamental plants all over the world, with three main distribution centers, the Central and western Asia/Mediterranean (CAM), the East Aisa (EA), and the Central and South America (CASA). Along with its large number of species and world-wide distribution, Salvia is paraphyletic with multiple diversity. Chloroplast genomes (CPs) are useful tools for analyzing the phylogeny of plants at lower taxonomic levels. In this study, we reported chloroplast genomes of five species of Salvia and performed phylogenetic analysis with current available CPs of Salvia. Repeated sequence analysis and comparative analysis of Salvia CPs were also performed with representative species from different distribution centers. The results showed that the genetic characters of the CPs are related to the geographic distribution of plants. Species from CAM diverged first to form a separate group, followed by species from EA, and finally species from CASA. Larger variations of CPs were observed in species from CAM, whereas more deficient sequences and less repeated sequences in the CPs were observed in species from CASA. These results provide valuable information on the development and utilization of the worldwide genetic resources of Salvia.
Antimicrobial activity of the Lacticaseibacillus rhamnosus CRL 2244 and its impact on the phenotypic and transcriptional responses in carbapenem resistant Acinetobacter baumannii
Authors: Rodriguez Cecilia , Ramlaoui Dema , Georgeos Nardin , Gasca Briea , Leal Camila , Subils Tomás , Tuttobene Marisel R. , Sieira Rodrigo , Salzameda Nicholas T. , Bonomo Robert A. , Raya Raúl , Ramirez María Soledad
Published: August 2023

Carbapenem-resistant Acinetobacter baumannii (CRAB) is a recognized nosocomial pathogen with limited antibiotic treatment options. Lactic acid bacteria (LAB) constitute a promising therapeutic alternative. Here we studied the antibacterial properties of a collection of LAB strains using phenotypic and transcriptomic analysis against A. baumannii clinical strains. One strain, Lacticaseibacillus rhamnosus CRL 2244, demonstrated a potent inhibitory capacity on A. baumannii with a significant killing activity. Scanning electron microscopy images showed changes in the morphology of A. baumannii with an increased formation of outer membrane vesicles. Significant changes in the expression levels of a wide variety of genes were also observed. Interestingly, most of the modified genes were involved in a metabolic pathway known to be associated with the survival of A. baumannii. The paa operon, Hut system, and fatty acid degradation were some of the pathways that were induced. The analysis reveals the impact of Lcb. rhamnosus CRL 2244 on A. baumannii response, resulting in bacterial stress and subsequent cell death. These findings highlight the antibacterial properties of Lcb. rhamnosus CRL 2244 and its potential as an alternative or complementary strategy for treating infections. Further exploration and development of LAB as a treatment option could provide valuable alternatives for combating CRAB infections.
The effect of masks on the emotion perception of a facial crowd
Authors: Cho Jieun , Im Hee Yeon , Yoon Young Jun , Joo Sung Jun , Chong Sang Chul
Published: August 2023

The present study investigated the effect of facial masks on people’s ability to perceive emotions in crowds. We presented faces with the bottom halves occluded by masks or full faces without occlusion. In two sequentially presented crowds, we varied the number of faces, emotional valence, and intensity of facial expressions, examining the impact of masks on the perception of crowd emotion. Participants reported which of the two crowds they would avoid based on the crowds’ average emotions. The participants’ ability to judge the average emotion of a crowd, especially a crowd expressing happiness, was impaired when the crowd wore masks. For faces covered by masks, crowd emotion judgments were more negatively biased than those without masks. However, participants could still distinguish the emotional intensities of a crowd wearing masks above chance. Additionally, participants responded more quickly to a crowd with more people without compromising accuracy, despite the perceptual challenges imposed by facial masks. Our results suggest that under ambiguous social situations in which individuals’ emotions are partially hidden by masks, a large group may provide stronger social cues than a small group, thereby promoting communication and regulating social behaviors.
Impact of physical and social living environments on pro-environmental intentions
Authors: Ngo Tuan-Hung , Lung Shih-Chun Candice
Published: August 2023

The living environment might play an important role in shaping the pro-environmental intentions of the people. However, there was limited research on how the living environments influenced the pro-environmental intentions of people. The objectives of this study are to evaluate the direct effects of physical and social environments on pro-environmental intentions as well as the mediating effects of environmental attitudes and life satisfaction. Structural Equation Modeling was used with data extracted from the 2020 Taiwan Social Change Survey database (n = 1671). Results showed direct positive associations of both physical and social environments with pro-environmental intentions (β = 0.133 and β = 0.076, respectively) as well as indirect positive associations via the life satisfaction-mediating pathway (β = 0.031 and β = 0.044, respectively). The physical environment negatively influenced pro-environmental intentions through the environmental attitude pathway (β =  − 0.255) with unpleasant neighborhood enhancing the pro-environmental intentions of residents. Taken together, the overall effect of the physical environment was negative (β =  − 0.093) while that of the social environment was positive (β = 0.109). The most important factors for the physical and social environments were disturbance and livability in north, central and south Taiwan, neighborhood pollution and interestingness in east Taiwan. Accordingly, minimizing disturbance and neighborhood pollution of the physical environment could have the highest effect on pro-environmental intentions enhancement in western and eastern Taiwan, respectively. For the social environment, improving livability in the west and interestingness in the east would have an even larger impact on pro-environmental intentions. This study emphasized the importance of neighborhood environment on the environmental intentions of the people. The study also identified the important factors for policymakers to target to achieve the best effect on improving environmental intentions.
No increase in translocated chromosomal aberrations, an indicator of ionizing radiation exposure, in childhood thyroid cancer in Fukushima Prefecture
Authors: Sakai Akira , Tsuyama Naohiro , Ohira Tetsuya , Sugai-Takahashi Misaki , Ohba Takashi , Azami Yusuke , Matsumoto Yoshiko , Manabu Iwadate , Suzuki Satoshi , Sato Maki , Hosoya Mitsuaki , Ishikawa Tetsuo , Suzuki Shinichi
Published: August 2023

To investigate the effects of radiation exposure due to the Fukushima nuclear power plant accident, following the disaster Fukushima Prefecture launched thyroid ultrasound examinations of residents who were generally younger than 18 years at the time of the earthquake. As the rate of pediatric thyroid cancer was higher than expected, we conducted biological dose assessment based on the frequency of translocated chromosome (Tr) aberrations using peripheral blood lymphocytes. Tr formation frequency was compared among the thyroid cancer (n = 38, median age 18 years, age range 12–26 years), thyroid-related disease (n = 30, median age 21 years, age range 15–28 years), and healthy controls (n = 31, median age 22 years, age range 20–23 years) groups. Tr aberration frequency was initially significantly higher in the thyroid cancer than in the other two groups; however, differences among the groups disappeared after adjusting for history of CT scan, as 92%, 67%, and 28% of those in the thyroid cancer, thyroid-related disease, and control groups, respectively, had undergone CT previously. Therefore, the significant difference in the initial number of Tr formations is presumably due to radiation exposure from CT. Accordingly, the effects of medical exposure on the chromosomes of children and adolescents should be noted.
Prevalence of anemia and associated factors among adult hypertensive patients in Referral Hospitals, Amhara Regional State
Authors: Gela Yibeltal Yismaw , Belay Daniel Gashaneh , Chilot Dagmawi , Andualem Amare Agmas , Bitew Desalegn Anmut , Sinamaw Deresse , Eshetu Habitu Birhan , Seid Abdulwase Mohammed , Simegn Wudneh , Kibret Anteneh Ayelign , Seid Mohammed Abdu , Diress Mengistie
Published: August 2023

Anemia is a risk factor for adverse cardiovascular disease outcomes in hypertensive patients. Chronic anemia increases preload, reduces afterload, and leads to increased cardiac output in hypertension patients. In the long term, this may result in maladaptive left ventricular hypertrophy, which in turn is a well-recognized risk factor for cardiovascular disease outcomes and all-cause mortality in hypertension. Low hemoglobin and hematocrit levels might be strongly indicate hypertensive end-organ damage, specifically kidney failure. Therefore, this study determined the prevalence of anemia and associated factors among hypertensive patients in Referral Hospitals, Amhara Regional State, Ethiopia, in 2020. An institution-based cross-sectional study was conducted in Amhara Regional Referral Hospitals from February 20 to April 30, 2020. Random and systemic sampling techniques were used to select 428 study participants. Data were entered and coded in to Epi data version 3.0 and then exported into STATA 14 for analysis. In bivariable logistic regression, variables with a p-value of < 0.25 were included in multivariable logistic regression. Using a 95% confidence interval, variables having a p-value ≤ 0.05 in multivariable logistic regression were declared as statistically significant variables. In this study, a total of 428 study participants were involved with 99.5% response rate. The prevalence of anemia among hypertensive patients was 17.6%, with a 95% CI (14.3–21.5%). Estimated glomerular filtration rate (eGFR) < 90 ml/min (AOR = 2.77, 95% CI 1.56- 4.92)], duration of hypertension (HTN) ≥ 5 years (AOR = 2.37, 95% CI 1.36–4.15), uncontrolled blood pressure (AOR = 1.91, 95% CI 1.08–3.35), and higher pulse pressure (AOR = 1.05 (95% CI 1.02–1.08) were significantly associated with anemia. Nearly one out of five hypertensive patients had anemia. Impaired estimated glomerular filtration rate, duration of HTN, blood pressure status, and pulse pressure were the independent predictors of anemia among hypertensive patients. Screening hemoglobin level at a regular interval is recommended for the hypertensive patients to take an appropriate intervention.
New cut-off points of PHQ-9 and its variants, in Costa Rica: a nationwide observational study
Authors: González-Sánchez Armando , Ortega-Moreno Raúl , Villegas-Barahona Greibin , Carazo-Vargas Eva , Arias-LeClaire Harold , Vicente-Galindo Purificación
Published: August 2023

The PHQ-9 questionnaire is a screening test worldwide used to measure depression. But it cannot be used in Costa Rica, due to the fact that it has not previously been validated for its population. The present study aims to show the validation of the PHQ-9 questionnaire and its variants (PHQ-2, PHQ-4, PHQ-8) in a population sample of adults residing in Costa Rica. A sample was collected (n = 1162) using a self-administered questionnaire. Confirmatory Factor Analysis (CFA), Receiver Operating Characteristic (ROC) curve, and Multiple Group Confirmatory Factor Analysis (MGCFA) were tested. One factor was found that explained 73.33% of the variance with excellent internal consistency (α = 0.928). Goodness-of-fit measures were adequate (RMSEA = 0.107; CFI = 0.948), as was diagnostic power at a cut-off of 10 (78.60 for Sensitivity and 27.95 for 1-Specificity). External validation indices were good (r = 0.843 with GAD-7, r = − 0.647 with RS14, and r = 0.301 with FCV19S), and the model showed invariance by sex (∆χ2 = 27.90; df = 27; p < 0.001). Additionally, new cut-off points were proposed for PHQ-9 and its variants for Costa Rican male, female, and general populations. The PHQ-9 and its variants (PHQ-2, 4, and 8) are valid tools for detecting depression (and anxiety for PHQ-4) in Costa Rican population. In addition, new cut-off points differentiated by sex are proposed.
Differentiable optimization layers enhance GNN-based mitosis detection
Authors: Zhang Haishan , Nguyen Dai Hai , Tsuda Koji
Published: August 2023

Automatic mitosis detection from video is an essential step in analyzing proliferative behaviour of cells. In existing studies, a conventional object detector such as Unet is combined with a link prediction algorithm to find correspondences between parent and daughter cells. However, they do not take into account the biological constraint that a cell in a frame can correspond to up to two cells in the next frame. Our model called GNN-DOL enables mitosis detection by complementing a graph neural network (GNN) with a differentiable optimization layer (DOL) that implements the constraint. In time-lapse microscopy sequences cultured under four different conditions, we observed that the layer substantially improved detection performance in comparison with GNN-based link prediction. Our results illustrate the importance of incorporating biological knowledge explicitly into deep learning models.
Dense bidisperse suspensions under non-homogeneous shear
Authors: Monti Alessandro , Rosti Marco Edoardo
Published: August 2023

We study the rheological behaviour of bidisperse suspensions in three dimensions under a non-uniform shear flow, made by the superimposition of a linear shear and a sinusoidal disturbance. Our results show that (i) only a streamwise disturbance in the shear-plane alters the suspension dynamics by substantially reducing the relative viscosity, (ii) with the amplitude of the disturbance determining a threshold value for the effect to kick-in and its wavenumber controlling the amount of reduction and which of the two phases is affected. We show that, (iii) the rheological changes are caused by the effective separation of the two phases, with the large or small particles layering in separate regions. We provide a physical explanation of the phase separation process and of the conditions necessary to trigger it. We test the results in the whole flow curve, and we show that the mechanism remains substantially unaltered, with the only difference being the nature of the interactions between particles modified by the phase separation.