bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrative analysis of SEPN1 in glioma: Prognostic roles, functional implications, and potential therapeutic interventions
Authors: Wang Zisong , Wang Danwen , Wang Xuanyu , Xu Yihang , Yuan Yunhe , Chen Yuxin , Li Zhiqiang , Liu Xiaoping
Published: February 2025

Background

SEPN1, a selenoprotein involved in redox regulation and endoplasmic reticulum stress response, has an unclear role in cancer. This study aims to investigate the expression, prognostic significance, and tumor microenvironment (TME) relevance of SEPN1 across pan-cancer, with a particular focus on glioma.

Methods

We analyzed SEPN1 expression and prognosis using the TCGA pan-cancer cohort. SEPN1 in glioma was further examined using data from TCGA, CGGA, GEO, and ZN-GC cohorts, along with survival analysis, single-cell RNA sequencing analysis, and enrichment analysis. We developed an SEPN1-related risk score (SRS) based on SEPN1-related long non-coding RNAs and validated its prognostic value. Drug sensitivity data and connectivity map analysis identified potential anti-glioma drugs based on the SRS.

Results

We found that SEPN1 was significantly upregulated in glioma, associated with poor prognosis, functioned as an independent risk factor, and predominantly expressed in malignant glioma cells. Enrichment analysis indicated the involvement of SEPN1 in immune-related processes and signaling pathways. Suppressing SEPN1 in glioblastoma cells inhibited proliferation and induced G2/M arrest and apoptosis. The SRS demonstrated strong prognostic value and correlated with enhanced immune infiltration in the glioma TME. Potential anti-glioma drugs were identified based on the SRS.

Conclusions

SEPN1 emerges as a novel biomarker and therapeutic target in glioma, providing a basis for future development of targeted therapies.
Composition, determinants, and risk factors of low birth weight in Sri Lanka
Authors: Mettananda Sachith , Herath Himali , Thewage Ayesha , Nanayakkara Kumudu , Liyanage Indeewari , Udani K. S. , Savanadasa Rajika , Goonewardena Sampatha , Gamhewage Nimesha , Hewamalage Asiri , Rowel Dhammica , Daniel Abner Elkan , de Silva Chithramalee , Perera Susie
Published: February 2025

Introduction

Low birth weight continues to pose significant challenges to healthcare systems worldwide. Despite substantial improvement in various public health indicators, many developing countries have failed to achieve a significant reduction in low birth weight rates. One major obstacle is the sparsity of data on the determinants of low birth weight. Here, we aim to determine the composition and risk factors for low birth weight in Sri Lanka, a prototype developing nation.

Methodology

We conducted a countrywide multicentre cross-sectional study in August and September 2023 in 13 purposively selected hospitals representing all nine provinces and different tiers of specialist hospitals in Sri Lanka. All live-born neonates were recruited prospectively, and their mothers were interviewed by trained data collectors to gather information on socio-demographic background, medical and obstetric history, and delivery details. Birth weight was measured immediately after the birth by trained healthcare personnel attending the delivery.

Results

A total of 9130 live-born neonates were recruited, of which 52% were males. The mean birth weight was 2827g (95%CI 2817-2838g), and 1865 (20.4%) newborns were low birth weight. The prevalence of prematurity was 10.9% (n = 998), and 1819 (20.0%) neonates were born small for gestational age. Of the low birth weight neonates, 64% were small for gestational age, and 37% were preterm; 11% were both small for gestational age and preterm. Teenage pregnancy (p = 0.022), low maternal pre-pregnancy body mass index (p<0.001), inadequate weight gain during pregnancy (p<0.001), maternal anaemia at delivery (p = 0.020), chronic lung disease (p = 0.019), and pregnancy induced hypertension (p = 0.019) were significant modifiable risk factors for small for gestational age.

Conclusion

This study presents the results of one of the most extensive country-wide studies evaluating the composition and determinants of low birth weight. The study highlights the importance of considering small for gestational age and prematurity as separate categories of low birth weight. Small for gestational age contributes to approximately two-thirds of the low birth weight burden. Therefore, targeting modifiable risk factors for small for gestational age while mitigating the burden of prematurity is the most feasible approach to reduce the prevalence of low birth weight in developing countries, including Sri Lanka.
Immunoinformatic strategy for developing multi-epitope subunit vaccine against Helicobacter pylori
Authors: Nahian Md. , Khan Md. Rasel , Rahman Fabiha , Reza Hossain Mohammed , Bayil Imren , Nodee Tanjum Ahmed , Basher Tabassum , Sany Mostafizur Rahaman , Munmun Rabeya Najnin , Habib S. M. Ariful , Mazumder Lincon , Acharjee Mrityunjoy
Published: February 2025

Helicobacter pylori is a gram-negative bacterium that persistently infects the human stomach, leading to peptic ulcers, gastritis, and an increased risk of gastric cancer. The extremophilic characteristics of this bacterium make it resistant to current drug treatments, and there are no licensed vaccines available against H. pylori. Computational approaches offer a viable alternative for designing antigenic, stable, and safe vaccines to control infections caused by this pathogen. In this study, we employed an immunoinformatic strategy to design a set of candidate multi-epitope subunit vaccines by combining the most potent B and T cell epitopes from three targeted antigenic proteins (BabA, CagA, and VacA). Out of the 12 hypothetical vaccines generated, two (HP_VaX_V1 and HP_VaX_V2) were found to be strongly immunogenic, non-allergenic, and structurally stable. The proposed vaccine candidates were evaluated based on population coverage, molecular docking, immune simulations, codon adaptation, secondary mRNA structure, and in silico cloning. The vaccine candidates exhibited antigenic scores of 1.19 and 1.01, with 93.5% and 90.4% of the most rama-favored regions, respectively. HP_VaX_V1 and HP_VaX_V2 exhibited the strongest binding affinity towards TLR-7 and TLR-8, as determined by molecular docking simulations (ΔG = −20.3 and −20.9, respectively). Afterward, multi-scale normal mode analysis simulation revealed the structural flexibility and stability of vaccine candidates. Additionally, immune simulations showed elevated levels of cell-mediated immunity, while repeated exposure simulations indicated rapid antigen clearance. Finally, in silico cloning was performed using the expression vector pET28a (+) with optimized restriction sites to develop a viable strategy for large-scale production of the chosen vaccine constructs. These analyses suggest that the proposed vaccines may elicit potent immune responses against H. pylori, but laboratory validation is needed to verify their safety and immunogenicity.
Musical-theatrical Global South roundtables: Selected excerpts
Authors: Asare Masi , Méndez Emilio
Published: February 2025

In January 2024, Masi Asare and Emilio Méndez convened three Zoom conversations with artists and researchers from around the globe on the topic of the ‘Musical-Theatrical Global South’. Below are excerpts from these conversations, lightly edited for clarity. These excerpted comments are collectively organized around five themes that recurred across the roundtables: (1) the relevance of the term musical in Global South contexts, (2) language and translation, (3) Indigenous and popular musical elements, (4) migration and transnational travel and (5) forms of musical-theatrical performance. This last theme, about form, can be understood to directly respond to and expand upon the first theme about terminology and ways of defining ‘the musical’.
Mexicano Flores
Authors: Romero-Herrera Claudia , Oliva Lorena
Published: February 2025

Mexicano Flores is a musical about the real-life trial and execution of Miguel Ángel Flores, an undocumented Mexican in Texas, who murders Angela, a US citizen. Both the victim and perpetrator aspired to be singers, but ended up bloodied in a car after a deplorable femicide. The musical also reflects on the 2000 US presidential elections, which coincided with the vote that sealed Miguel’s execution. Influenced by both Brecht and Broadway, Mexicano Flores features a traditional Mexican lullaby and nine original songs by Lorena Oliva and Claudia Romero Herrera, orchestrated in the 1980s and 1990s style to orient ourselves in time and to contrast the play’s dense content. From its conception, Mexicano Flores envisioned a farcical but intimate and reflexive atmosphere, as an alternative to the conventional musical that bases its appeal on the lavishness of the production and choreography. Mexicano Flores argues that no murder can be the solution to a problem, neither the one that takes place in the darkness of anonymity nor the one that occurs under the intense light of legality. In this fragment of Mexicano Flores, a clownish representative of the Mexican government begs for mercy to a George W. Bush who is only interested in becoming President-elect, and who ultimately proceeds with Flores’ execution. Flores bids farewell to his family and has a last imaginary encounter with Angela. He sings a repentance song to silence Angela’s voice in his head, and is afterwards transported by the chorus to the first encounters with her, back in 1989, to discover his real motivations to carry out the femicide.
Propuesta de actividades para trabajar obras de música clásica en Primaria
Authors: Belando Lajarin María José
Published: February 2025

la Historia de la Música en Educación Primaria sigue siendo desafío vigente, ya sea debido a su complejidad o a la falta de conocimiento. A pesar de la presencia de la música clásica en la vida cotidiana del alumnado, la falta de información sobre sus orígenes crea una barrera que dificulta su comprensión. Este estudio tiene como objetivo diseñar una serie de actividades que aborden los fundamentos esenciales de la Educación Musical y la Danza a través de obras clásicas. La finalidad es proporcionar herramientas para desmitificar la música clásica en las aulas y permitir su enseñanza de manera lúdica y significativa. Para lograr este propósito, se ha llevado a cabo una revisión de la presencia y evolución de la música clásica en los planes de estudio a nivel nacional y regional, junto con un análisis de los períodos musicales, obras y compositores más destacados. Como resultado, se han seleccionado y adaptado las obras más relevantes para su utilización en el aula. El resultado de esta investigación son siete actividades secuenciadas en orden cronológico destinadas a estudiantes de cuarto curso.
El musicograma y su implementación en el aula de Educación Secundaria
Authors: Hernández Meseguer José David
Published: February 2025

En esta investigación se expone el potencial y utilidad del musicograma como recurso educativo en el contexto de la Educación Musical en Educación Secundaria. Se lleva a cabo una revisión del concepto de musicograma y sus principales características. A continuación, se presentan ocho musicogramas de elaboración propia que atienden a diversos aspectos de la Educación Musical, como la percusión corporal, la instrumentación y el movimiento. Estos se basan tanto en músicas del ámbito culto o clásico, como en músicas actuales, para comprobar su impacto en la predisposición y motivación del alumnado hacia las actividades. Los resultados obtenidos indican que el 85% de los estudiantes mostró un aumento en su interés por la música al utilizar los musicogramas, y se observó una mejora del 30% en la coordinación y habilidades rítmicas en comparación con clases anteriores. Por tanto, se puede concluir que el uso de músicas contemporáneas contribuyó a una mayor implicación, lo que sugiere que la adaptación de los contenidos a los gustos actuales de los alumnos puede enriquecer su experiencia educativa.
Música y emociones: el rap como instrumento educativo para fomentar la conciencia emocional y la expresión de emociones
Authors: Garrido Villa Miriam
Published: February 2025

Durante la primera infancia comienzan a moldearse los cimientos emocionales, pero es en la adolescencia cuando se consolidan y fortalecen, convirtiéndose el centro educativo en un lugar crucial para el desarrollo y adquisición de recursos emocionales. Esta propuesta de innovación tiene como objetivo diseñar una propuesta didáctica que ayude en el desarrollo de la conciencia emocional y la expresión de emociones a través de la música rap, en los estudiantes de segundo curso de la Educación Secundaria Obligatoria (ESO). Por ello, se presenta un marco teórico dividido en dos ejes: la inteligencia emocional y su contexto en el ámbito de la ESO y la música rap como herramienta educativa. Esta propuesta fue llevada a la práctica en el mes de abril de 2024, consiguiendo unos resultados óptimos puesto que fueron logrados los objetivos de aprendizaje establecidos, subrayando así el potencial de la música rap como herramienta educativa para el desarrollo emocional.
Aortoduodenal Syndrome: A Rare Case of Duodenal Obstruction Due to Endovascular Aneurysm Endoleak
Authors: Adhikari Raksha , Patel Parth , Tait Christopher
Published: February 2025

Aortoduodenal syndrome results from expansion of an abdominal aortic aneurysm, leading to symptoms of upper gastrointestinal obstruction. Endovascular aneurysm repair of large or symptomatic aortic aneurysms has become an important treatment modality for patients with abdominal aortic aneurysm. However, a large proportion of these grafts are complicated by endoleaks that can lead to a spectrum of significant symptoms. We present a case of rapidly worsening gastrointestinal obstructive symptoms in a patient with an already repaired aortic stent graft and a solitary kidney, found to be due to a type III endoleak that improved after emergent repeat endovascular aneurysm repair.
The rise and fall of Jackdaws: lessons for designing source collections to teach history
Authors: Gibson Lindsay
Published: February 2025

For more than a century, educators and historians have advocated for the importance of using primary sources for teaching history. One of the most innovative and popular collections of sources in the past 60 years were Jackdaws: Collections of Contemporary Documents, which were published by Jonathan Cape between 1963 and 1977. Jackdaws are folders that contain reproductions of primary and secondary sources focused on significant historical events, people, developments, themes and topics in history. In this article, I provide a brief history of Jackdaws, and explain why they were initially popular as a learning resource for teaching history, and why their popularity waned in the mid-to-late 1970s. I conclude by highlighting several lessons that can be learned from the rise and fall of Jackdaws that might help history teachers and educators design collections of primary and secondary sources for teaching history. The three reasons that best explain why Jackdaws became popular learning resources in school history classrooms between 1963 and 1977 are that they were aligned with innovative educational theories at the time, they were flexible and adaptable to diverse contexts, and they were interesting and exciting for students. Despite being heralded as a groundbreaking and revolutionary resource for teaching history, Jackdaws failed to transform history teaching and learning for four main reasons: they were too difficult for some students; they were an awkward fit for some school history curricula; they were expensive and difficult to manage; and there was a lack of pedagogical supports to help teachers use them effectively.
The Influence of Environmental Variables on the Distribution of Mangroves in Northeast Brazil
Authors: De Sá Ana Caroline Damasceno Souza , Pinheiro Lidriana
Published: February 2025

This research aims to spatialize mangroves and correlate them with environmental variables, exploring potential variation in the distribution of mangrove and salt flat forests in relation to areas with different rainfall patterns and drought periods within the same Brazilian region. CBERS-4A satellite images and manual vectorization of mangrove and apicum forest polygons in QGIS v. 3.10.14 software were used, as well as fieldwork. Pearson's correlation analysis, carried out using RStudio software, considered variables such as rainfall, dry months, mangrove species, fragmentation, and area size. The results indicate that variations in rainfall and the number of dry months have a strong influence on the distribution of mangroves. For example, in the Piranhas-Açu hypersaline estuary (RN), a large fragmentation of mangrove forests was observed, with 1.379 polygons mapped in an area of 2.396 ha, receiving 500 mm of annual rainfall and having 8 dry months. In the Timonha (CE) and Itarema (CE) estuaries, with 1000 mm of rain and 6 dry months, 131 and 118 polygons were identified, respectively, on 2482 and 1093 ha. In contrast, the Mamanguape River estuary (PB), with the highest annual rainfall of 1750 mm and only 3 dry months, has a distribution of 4256 ha in 24 polygons. As for the salt flats, the Piranhas-Açu (RN) has a fragmentation of 674 polygons in an area of 4858 ha and the Mamanguape River estuary (PB) has six polygons mapped in an area of 2.4 ha. The research highlights the impacts of climatic variables on the distribution of mangroves, anthropogenic interference in land use due to economic activities and the negative effects of fragmentation, underscoring the importance of conservation and proper management of these vital ecosystems.
The Contribution of the FPEIR/TOPSIS Model in The Environmental Diagnosis of Water Security in Areas Affected by the B1 Dam, Brumadinho, MG
Authors: Honorato Priscila Aparecida da Rocha , Elmiro Marcos Antonio Timbó , Nero Marcelo Antonio , Temba Plinio da Costa , Jardim Helder Lages
Published: February 2025

The Paraopeba River Basin, located in the state of Minas Gerais, Brazil, has been severely impacted by extreme climatic events and the 2019 Vale S.A. dam failures in Brumadinho. This study underscores the importance of integrating multi-criteria decision analysis with a conceptual model to diagnose water security in the region based on socio-environmental indicators. Employing the DPSIR methodology, socio-environmental indicators are proposed and analyzed to assess water security and to support the planning and management of water resources in the Paraopeba River Basin (BHRP). The approach combines the TOPSIS multi-criteria analysis model with the entropy weight method to rank municipalities according to their levels of water security. The analysis identified critical challenges, including population growth, industrialization, and agricultural expansion, which exacerbate water contamination. Brumadinho and Betim emerged as the most vulnerable municipalities, exhibiting high rates of waterborne diseases and inadequate wastewater treatment infrastructure. Intense industrial and agricultural activities have further heightened environmental risks, as exemplified by the dam failures. The study underscores socio-environmental pressures and highlights the importance of integrated approaches. The findings emphasize the need for robust public policies, sustainable agricultural practices, participatory management, infrastructure improvements, and climate change adaptation to ensure water security and improve quality of life.
Relación entre sensibilidad al refuerzo, desesperanza e ideación suicida en adultos colombiano
Authors: Alonso Pacheco Daniela , Arévalo Moreno Laura Melissa , D'Achiardi Gómez Luisa Fernanda , López Niño Yuliana Andrea , Rojas Vargas Pedro Camilo , Toro Ronald
Published: February 2025

Las altas cifras de suicidio a nivel mundial y nacional, así como los altos índices de intentos suicidas en Colombia, hace que sea importante identificar los factores causales de este problema. El objetivo del estudio fue establecer la relación entre la sensibilidad al refuerzo (sistema de inhibición conductual [BIS, por sus siglas en inglés] y sistema de activación conductual [BAS, por sus siglas en inglés]), desesperanza e ideación suicida en adultos colombianos. Se realizó un estudio de enfoque empírico cuantitativo con una estrategia correlacional. La muestra fue no probabilística por conveniencia incidental de 645 participantes, a quienes se les aplicaron los instrumentos Inventario de Desesperanza e Ideación Suicida (IDIS) y la Escala BIS-BAS versión IPIP-R. Los datos fueron recolectados mediante un aplicativo virtual, lo cual facilitó la búsqueda de datos. El análisis estadístico fue llevado a cabo a través del Jeffrey’s Amazing Statistics Program (JASP). En los resultados, se obtuvo una correlación positiva entre el BIS y la desesperanza e ideación suicida, y una correlación inversamente proporcional entre el BAS, la desesperanza y la ideación suicida. Se pudo concluir que, cuanto mayor BIS, mayor ideación suicida, y que cuanto mayor desesperanza, mayor ideación suicida.
Farmers’ awareness on bio inputs in coconut cultivation based on education in Thondamuthur block of Coimbatore district, Tamil Nadu, India
Authors: Manikandan P , Sivakumar S D , Velavan C , Karthikeyan S , Vanitha G
Published: February 2025

This study investigated farmers’ awareness and use of bio-inputs in coconut farming in Thondamuthur block, Coimbatore district, Tamil Nadu, India. Given agriculture's significance in India’s economy, bio-inputs are an alternative to chemical fertilizers amidst population increase and climate change. The study evaluates the level of awareness amongst farmers, factors that influence the buying behaviour of bio-inputs like bio-fertilizers and bio-pesticides and constraints to their use. Statistical analysis included percentage analysis, Chi-square test and factor analysis on data from 140 respondents. Factors like yield, price, experience, knowledge and government subsidies influenced buying behaviour mainly. The findings revealed that although farmers are aware of bio-inputs, constraints such as availability, poor government support and quality concerns limit their utilization. The paper emphasized that given these constraints, there is a need for more policy support as well as training programs to enhance bio-input utilization in sustainable agriculture in the study area.
Unveiling the multifaceted attributes of Bixa orellana: An insight into its chemical composition, industrial application and cultural significance
Authors: Devanand P S , Radha P , Kumar P , Sivakumar B , Sivaprakash M , Kiruba M , Raja N , Utharasu S , Mangammal P , Anand G , Mathivanan M , Revathi R
Published: February 2025

Bixa orellana L., commonly known as annatto or the lipstick tree, is an evergreen shrub from the Bixaceae family. Native to tropical regions of Central and South America, it is now cultivated globally. Renowned for its striking red flowers and spiny fruits, B. orellana seeds produce annatto, a natural dye rich in bixin and norbixin pigments. These water- and oil-soluble carotenoids are widely used as natural food colorants, especially with the rising demand for synthetic dye alternatives. Beyond its industrial applications in food, cosmetics, leather and solar cells, B. orellana has traditional medicinal uses for treating conditions like gonorrhea, asthma and sore throats. Its bioactive components, including carotenoids and essential oils, offer antioxidant and hypoglycemic properties. This review highlights the pharmacological activities, phytochemical composition and industrial relevance of B. orellana, showcasing its diverse economic, botanical and cultural significance.
Inheritance of fertility restoration in A4 cytoplasm of Pigeonpea (Cajanus cajanifolius L.)
Authors: Thanga Hemavathy Arumugam , Kavitha Shanmugam , Sakila Muthusamy , Vijaya Geetha Venugopal , Kanimoli Mathivathana.M , Samyuktha Santhi Madhavan
Published: February 2025

A set of three medium-duration CMS lines ICPA 2043, ICPA 2047, and ICPA 2048 were used in study to investigate fertility restoration by making twenty-eight test crosses at the Department of Pulses, TNAU, Coimbatore. ICPA 2047 exhibited fertility restoration ranging from 0-40 % with negligible pod setting except for the cross ICPA 2047×ICP 3181. In contrast, sixteen F1s derived from ICPA 2048 showed pollen fertility ranging from 10-48 % except for the cross ICPA 2048 × ICP 9224. Similarly, sixteen F1s derived from ICPA 2043 demonstrated 15-38 % pollen fertility, with ICPA 2043 × LRG 41 showing 98 % fertility and good pod setting. Among the hybrids, ICPA 2043 × LRG 41 exhibited high fertility, ranging from 95.58 % to 98.67 % over two years. This suggests that dominant fertility-restoring genes from the restorer parent were incorporated into the hybrid, which showed consistent fertility restoration across different years. In the current investigation, the restorer line ICP 3181 demonstrated monogenic inheritance of fertility restoration in crosses with ICPA 2047 and ICPA 2048, following 3:1 ratio. Conversely, the cross ICPA 2043 × LRG 41 exhibited a digenic duplicate dominance inheritance pattern. These results suggest that the fertility restoration in pigeonpea can be inherited monogenically or di-genically, depending on the hybrid. The identified hybrids, particularly ICPA 2043 × LRG 41 demonstrate significant potential for improving fertility restoration and enhancing productivity in pigeonpea breeding programs.
Correlation of radiographic and histopathological changes with IL-17 and advanced oxidation protein products in knee osteoarthritic individuals with metabolic syndrome
Authors: Awan Uzma Naseer , Waraich Rizwana Sanaullah , Noor Syed Shahid , Siddiqui Iftikhar Ahmed , Nangrejo Ruqaya
Published: February 2025

Objectives

Recent studies show that osteoarthritis and metabolic syndrome (MetS) represent significant global health concerns, sharing common pathological processes involving inflammation and oxidative stress. The study aimed to compare the radiological and histological severity of osteoarthritis in patients with and without MetS and further correlates them with oxidative stress and inflammatory markers in serum and synovial fluid (SF). Hypothesis: The study hypothesized that IL-17 and advanced oxidation protein products (AOPPs) are correlated with OA severity and progression in MetS patients.

Methods

This cross-sectional study included 78 patients of advanced knee osteoarthritis, 40 with MetS and 38 without, matched for age. Clinical history and anthropometric measurements were recorded, and presurgical knee X-rays were evaluated using the Kellgren–Lawrence system. Histological grading of hematoxylin & eosin stained infrapatellar fat pad (IFP) and cartilage sections was performed. AOPPs and Interleukin-17 levels were measured in serum and SF, employing sandwich enzyme-linked immunosorbent assay.

Results

In the MetS group, the severity of osteoarthritis was higher compared to non-MetS group, as evidenced by histological evaluation of the articular cartilage and IFP (p<0.05). The histological grading of IFP demonstrated positive correlation (p<0.05) with histological cartilage grade. Additionally, it exhibited a positive correlation with interleukin-17 and AOPPs in both SF and serum (p<0.05). While histological cartilage grade showed a positive correlation with AOPPs concentration in the serum and SF (p<0.05).

Conclusions

MetS accelerates osteoarthritis progression, and positive correlation between molecular markers and histological severity suggests the contribution of inflammation and oxidative stress in the disease’s pathogenesis.
Papal Representatives in Hungary in the Eleventh–Fourteenth Centuries
Authors: Kiss Gergely , Báling Péter
Published: February 2025

Traditionally, research on Papal-Hungarian relations has been dominated by a diplomatic approach. Until the early 2000s, Hungarian historiography was traditionally characterised by a relatively schematic concept of papal representation, i.e. papal representatives were mostly understood as legates or nuncios. The last two decades have seen major changes in this area: the prosopographical research of papal representatives has begun, which has shed light on the various forms of representation (legates, nuncios, papal delegated judges, conservators, executors, administrators, papal collectors), and the thematic analysis of the relationships has become dominant. This paper presents the results of the DeLegatOnline research projects that bring together these studies.
The Authority of the Church in Seventeenth and Eighteenth-Century Catholic Literature of Bosnia and Herzegovina
Authors: Kovačić Iva Beljan
Published: February 2025

The Catholic Reformation and Counter-Reformation led to extensive religious and cultural activity among Catholics in Ottoman Bosnia and Herzegovina. The initiators of these actions were the clergy, primarily the Franciscans of the Bosna Srebrena province. A significant aspect of their work was the publication of religious literature, with the issue of Church authority being one of its central themes. The first part of this paper aims to examine the representation of the Church and its authority in literature derived from Latin and Italian sources. The second part addresses the distinct characteristics of Bosnian-Herzegovinian Catholicism in the early modern period. Given that these particularities are especially evident in the way Church authority functioned, this raises the question of coherence between the concept of Church authority as articulated in religious literature and its practical implementation.
The Social Structure of the Transylvanian Rural Society at the Beginning of the Eighteenth Century
Authors: Bandi K. Bálint
Published: February 2025

The history of the Principality of Transylvania is one of the most popular topics in Hungarian historiography and is also of great international interest. Although our understanding of the principality has significantly improved in recent decades, the overall perception of the subject has not fundamentally changed.The studies aimed at achieving a deeper and more comprehensive understanding of the nations and communities of Transylvania have a long history; however, our knowledge remains incomplete in many respects. While we have detailed descriptions of the social structure of Transylvanian society and its unique self-organizing communities during the Middle Ages, there is a notable lack of analysis concerning social relationships in the centuries following the Battle of Mohács. This is particularly true regarding the structural composition of rural society.This paper aims to analyse the structural characteristics of rural society and conduct a comparative examination of its composition in Cluj and Turda Counties during the early decades of the eighteenth century. By analysing the relevant data, we can gain a detailed understanding of the social relations within the rural communities of the period, while also providing a terminological framework for examining the conceptual development of given social categories.
Neoliberalismo e o retrocesso no processo de democratização na África: os ajustes estruturais na Guiné-Bissau
Authors: Indjai Mamadú
Published: February 2025

Resumo: Desde os últimos vinte anos do século passado, o debate sobre a democracia e o processo de democratização na África contemporânea tem se constituído por uma série de questões que o acompanham no continente. Esse debate envolve, sobretudo, o contexto e momento em que a democracia chegou à África, promovida por uma proposta de abertura democrática dentro das políticas neoliberais de privatizações, desregulamentações e da redução do papel do Estado em questões de assistencialismo nas áreas da educação e saúde. É neste sentido que esta proposta busca como objetivo compreender o neoliberalismo a partir das suas mudanças na ideologia e na prática a nível geopolítico, possibilitando (re)interpretações da interligação do neoliberalismo com o processo de democratização na África, estudando especificamente um projeto desenvolvido pelo Instituto Nacional de Estudos da Guiné-Bissau (INEP), o projeto de Estudos sobre Programas de Ajustamento Estrutural no país.
Tabú da democracia?! Solidariedade multiétnica e luta pelo poder
Authors: Sambé Jeraldino António , Carvalho Ricardo Ossagô de
Published: February 2025

Este artigo objetiva-se analisar como a solidariedade multiétnica e sua articulação política articulada dentro do contexto da arena do poder na Guiné-Bissau, buscando compreender as dinâmicas e tensões em volta da identidade coletiva fase as ameaças da etnicidade, clientelismo, politização, e luta pela manutenção dos recursos políticos e sua legitimação como regra do jogo. Em relação à metodologia, foi utilizada a revisão da literatura e abordagem qualitativa desse fenômeno a partir de estudos nacionais e internacionais. O desenrolar desse mecanismo político numa sociedade fragilizada como a bissau-guineense, tem impactado a configuração democrática, abrindo assim as portas para instabilidade governativa e política de alianças clientelistas e transformando-se nas ferramentas inevitáveis que subsidiam toda a dinâmica política contemporânea guineense.  Nesta conjuntura, a Guiné-Bissau enquanto país com uma rica diversidade étnica formada pelas complexidades socioculturais e históricas, paradoxalmente as articulações da etnicidade no campo político e das relações de poder tem movido a política nos últimos anos, como demonstra algumas literaturas, estes procedimentos da etnicidade, politização e clientelismo comprometem arena democrático guineense.
Revistando as reformas administrativas na Guiné-Bissau: uma descrição e análise do caso do PARAP
Authors: Carlitos Nhaga Ivaldino , De Souza Coelho Fernando
Published: February 2025

Este artigo descreve, de forma cronológica, o processo de implementação do Programa de Apoio à Reforma da Administração Pública (PARAP) na Guiné-Bissau, iniciado em 2008 pelo governo guineense, através do apoio da União Europeia. O estudo de caso aqui apresentado, resultado de uma dissertação de mestrado, responde a três questões centrais: como o programa começou, por que terminou e quais foram seus resultados. Utilizou-se a Estrutura Narrativa de Barzelay et al. (2001), composta por cinco eventos-chave (episódio, antecedentes, contemporâneos, relacionados e posteriores), para orientar a pesquisa sobre formulações de políticas de gestão pública. Ademais, este estudo, também se baseia em análise de algumas bibliografias, documentos oficiais e entrevistas semiestruturadas com atores-chave. Os resultados apontam que, apesar de desafios, o PARAP influenciou algumas mudanças na gestão pública, como a adoção de novas tecnologias de gestão de recursos públicos, atualizações legislativas e a transformação do CENFA em ENA.
Apresentação do dossiê “Democracia e governança na Guiné-Bissau: entre a instabilidade política e os desafios do desenvolvimento”
Authors: De Borba Barreto Alvaro Augusto , Anós Té Paulo
Published: February 2025

Texto de Apresentação do dossiê “Democracia e governança na Guiné-Bissau: entre a instabilidade política e os desafios do desenvolvimento”.
Strengthening Healthcare Cybersecurity: Leveraging Multi-Cloud and AI Solutions
Authors: Pendyala Santhosh Kumar
Published: February 2025

The increasing reliance on digital systems in healthcare has heightened the need for robust cybersecurity frameworks to safeguard sensitive
patient data and ensure uninterrupted service delivery. Healthcare institutions face unique challenges, including compliance with stringent
regulatory standards, integration of disparate data systems, and mitigating evolving cyber threats. The adoption of multi-cloud architectures
combined with artificial intelligence (AI) offers a promising solution to these challenges. Multi-cloud environments provide scalability, redundancy,
and resilience, while AI enhances threat detection, response capabilities, and data analytics.This study explores the synergy between multi-cloud
and AI technologies in fortifying healthcare cybersecurity. It highlights how hybrid cloud models optimize data management and security, and
how AI-driven tools, such as machine learning-based anomaly detection and natural language processing (NLP), identify and mitigate potential
vulnerabilities. Additionally, blockchain integration is discussed as a complementary approach to ensure data integrity and secure transactions.
The proposed framework incorporates advanced tools like TensorFlow for AI-driven anomaly detection, Azure Synapse for scalable multi-cloud
storage, and blockchain for immutable data security. The methodology emphasizes proactive threat management, real-time analytics, and regulatory
compliance.Through a comprehensive literature review and methodological analysis, this paper provides actionable insights into developing a robust
cybersecurity strategy for healthcare organizations. The proposed solutions aim to enhance data protection, optimize operational efficiency, and
support regulatory adherence, ultimately ensuring the secure and effective delivery of healthcare services in an increasingly digital landscape.In an
era where digital transformation is revolutionizing healthcare delivery, the industry faces unprecedented cybersecurity challenges. With sensitive
patient data, critical infrastructure, and life-saving medical devices all connected to networks, healthcare organizations must adopt robust security
measures. This article explores how multi-cloud strategies and AI-driven solutions are reshaping healthcare cybersecurity
Formation of a Model for Collecting and Processing Survey Data (on the Example of a Survey of Consumers of Educational Services)
Authors:   Lviv Polytechnic National University, Shuliar Roman , Horyslavets Pavlo ,   Lviv Polytechnic National University, Martseniuk Andrii ,   Lviv Polytechnic National University
Published: February 2025

The collection of data on consumer satisfaction with products and services is an important element of the marketing mix of
organizations. Higher education institutions (HEIs) in Ukraine are in the process of developing internal quality assurance
systems. An integral part of these systems is the surveying of students as a procedure for obtaining objective information. The
implementation of a specific survey model as an element of management and quality assurance systems for products, services,
and education in particular, is an important competence and responsibility of organizations. The task of establishing feedback
with consumers and taking their opinions into account when making management decisions is a relevant challenge for
organizations. Various models, which include survey schedules, their types, target audiences, questionnaire formats, and
platforms for conducting surveys, allow for the search for optimal approaches to these processes. The purpose of the article is to
investigate the factors influencing the active participation of consumers, particularly students, in surveys regarding the quality of
products and services and their alignment with expectations.
Gen Z sustainable consumption: evolution during and after COVID-19
Authors: Nava Rogel Rosa María
Published: February 2025

Given the power of decision and persuasion that Generation Z is acquiring, it is important to know the changes in the motivations they have towards sustainable consumption. This research analyzes the evolution of sustainable food consumption factors in Centennials during and after the pandemic, based on the Theory of Planned Behavior, which has been widely used to understand this phenomenon. In the first four months of 2021 and 2023, 662 young people born between 2000 and 2007 were surveyed; with the data collected, regression equations were calculated for each year, demonstrating a significant change in its structure with the Chow Test. During the pandemic and because of their young age, Generation Z had fewer possibilities to decide; in the new normal they acquired more freedom, increasing their purchase intention. This longitudinal study shows that the basic postulates of the Theory of Planned Behavior are fulfilled, if individuals have decision-making power and freedom to select what they prefer.
QUANTITATIVE INDICATORS OF COPPER-RESISTANT MICROORGANISMS DISTRIBUTION IN NATURAL ECOSYSTEMS
Authors:   Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv, Havryliuk O. А.
Published: February 2025

Copper is a highly toxic metal common in both natural and man-made ecosystems. The goal of the work was to determine the level of resistance of microorganisms of natural ecosystems to cationic form and organometallic complex of Cu2+. Microorganisms of 9 natural ecosystems of five geographic zones (the Antarctic, the Arctic, the Dead Sea (Israel), middle latitude (Ukraine) and the equatorial zone of South America (Ecuador) were investigated. Resistance of microorganisms was determined by cultivation in the medium with concentration gradient of Сu2+. The amount of Cu2+-resistant microorganisms in natural ecosystems was determined by colony counting on nutrient agar with Сu2+ citrate and Cu2+ cation. The Cu(II) concentration in soil and clay samples was analyzed by atomic absorption spectroscopy method. We have confirmed the hypothesis that microorganisms resistant to toxic Cu2+ compounds in high concentrations exist in any natural ecosystem. The resistance to Cu2+ cation was 8 – 31 and 14 –140 times less than to Cu2+ citrate in nutrient and mineral agar media respectively. The amount of Cu2+-resistant microorganisms in natural ecosystems reached hundreds and thousands at the presence of 175…15 500 ppm Cu2+. Thus, the soils, clays and sands of natural ecosystems are a “genetic resource” of copper-resistant microorganisms that are promising for development of novel biotechnology of purification of copper-containing wastewater and soil bioremediation.
FORMATION OF THE EUBACTERIAL COMPLEX IN THE RHYOSPHERE OF SUGAR BEET (Beta vulgaris) UNDER DIFFERENT FERTILIZATION SYSTEMS
Authors:   Taras Shevchenko National University of Kyiv, ESC "Institute of Biology and Medcine", Ukraine, Hudz S.
Published: February 2025

The aim of the work was to determine the quantitative, qualitative composition and taxonomic structure of the eubacterial complex in the rhizosphere of sugar beet under different fertilizer systems.

Microbiological methods were used to determine the content of microorganisms in the rhizosphere of sugar beet. Molecular methods were used to determine taxonomic structure as well as metagenome of the eubacterial complex of microorganisms.

In the agrocenosis of sugar beet under different fertilizer systems the representatives of such families were prevailed as Alcaligenaceae, Pseudomonadaceae, Nitrososphaeraceae, Gaiellaceae, Micrococcaceae, Solirubrobacteraceae, Streptomycetaceae, Intrasporangiaceae, Solimonadaceae, Syntrophobacteraceae, Xanthomonadaceae, Enterobacteriaceae, Nocardioidaceae, Hyphomicrobiaceae, Comamonadaceae. It was found that under the biological system of fertilizers the species diversity of soil microbiota increased due to phyla: Alcaligenaceae, Gaiellaceae, Solirubrobacteraceae, Streptomycetaceae, Solimonadaceae, Syntrophobacteraceae, Xanthomonadaceae, Enterobacteriaceae, Nocardioidaceae, Hyphomicrobiaceae, Hyphomicrobiaceae.

It was detected that the basis of the eubacterial complex of sugar beet included representatives of phyla Proteobacteria, Actinobacteria, Gemmatimonadetes, Chloroflexi, Acidobacteria, Firmicutes, Planctomycetes, Verrucomicrobia, Bacteroidetes, and the absolute dominants were Proteobacteria – 76.9%, Actinobacteria – 13,4 %.
The influence of introduced tree species on the relationships between soil buffering capacity and ecological groups of microorganisms in urban park soils
Authors: Didur O. O. , Katsevych V. V. , Ivanko I. A. , Loza I. M. , Strepetova K. V. , Holoborodko K. K. , Lovynska V. M. , Sytnyk S. A.
Published: February 2025

As a component of urban parks, soil is an essential part of urban green infrastructure; soil influences the green plantings in megacities, being affected by environmentally unfavorable factors. To reveal relationships between soil buffering capacity and ecological microbial groups of park soils, soil acid-base buffering capacity (pHBC) was determined with the Arrhenius method. We counted the soil microorganisms using differential diagnostic nutrient media with serial dilutions. Soil microbial biomass was determined by thermal rehydration. In statistical data processing, we used the methods of descriptive statistics, univariate analysis of variance, etc. We established strong positive correlations between pH values and pHBC in the acid and acid-base range, and a negative correlation between pH values and buffering capacity in the alkaline range; a strong correlation between the abundancies of amylolytic and pedotrophic soil microorganisms. We determined the effect of the three following soil sustainability-associated latent factors: Factor 1 – effect correlated with total pHBC, pHBC in the acid range and soil acidity, Factor 2 and Factor 3 – effects characterized by the direction of microbial processes and which formed a balanced system between the soil humus decomposition and the entry of plant litter. We revealed a beneficial impact of subcrown space on the abundancies of soil microorganisms. We recommend using acid and acid-base buffering capacity and microbiological characteristics as soil quality indicators. The abundance of soil microorganisms increased in the forested sites.