bluuebunny/crossref_metadata_2025 huggingface dataset:
Análise das notificações de violência de repetição em mulheres no estado do Ceará-Sinan, 2014-2019
Authors: Coelho Ananda Caroline Vasques Dantas , Silva Lucilane Maria Sales da , Neves Ana Vitória Pinheiro , Sousa Silvana Maria de Oliveira , Medina Ligia Lucena Gonçalves
Published: November 2021

Objetivo: Analisar as notificações de violência de repetição no estado do Ceará. Metodologia: Estudo descritivo, retrospectivo com abordagem quantitativa. Os dados foram coletados na base de dados DATASUS/SINAN. Resultados e Discussão: Foram notificados 6.942 casos de violência contra mulher em idade fértil de 10 a 49 anos no estado do Ceará. Conclusão: O ciclo da violência contra as mulheres em idade fértil no Ceará permaneceu inclusive após o registro das notificações.
O perfil clínico e o manejo terapêutico da síndrome HELLP: revisão integrativa
Authors: Alves Ana Klara Rodrigues , Silva Barbara Beatriz Lira da , Alves Francisco Robson de Oliveira , Silva Larissa dos Santos , Machado Lívia Filomena Castelo Branco , Pinho Alvaro Martins , Franklin Fábio Landel Alysson Araújo , Ibiapina Maria de Lourdes Pereira , Noronha Francisco Matheus Carvalho , Almeida Lia Moreira de Vasconcelos , Gonçalves Savir Igor de Oliveira , Medeiros Amanda Milhomem , Fortes Neto Noé Araujo , Bucar Luiza Emiliana Queiroz , Rodrigues Iluska Guimarães
Published: November 2021

O presente estudo tem como objetivo descrever o perfil clínico e o manejo terapêutico da síndrome HELLP. O estudo utilizou como método a revisão integrativa da literatura, realizado por meio de uma busca na base de dados Pubmed usando os cruzamentos os descritores em inglês “HELLP syndrome”, “complications”.  Para a avaliação do problema de pesquisa e sua estratificação foi utilizada a estratégia PVO. A estratégia supracitada permitiu formular a seguinte questão norteadora: Quais são as manifestações clínicas da síndrome HELLP e como funciona o manejo terapêutico? A fisiopatologia da doença ainda não é conhecida. No entanto, acredita-se que seja causada por insuficiência placentária e disfunção endotelial generalizada. A apresentação vaga é comum; hipertensão e proteinúria que caracterizam a pré-eclâmpsia geralmente estão ausentes quando a síndrome HELLP se apresenta. Pode-se apresentar com sinais e sintomas inespecíficos, nenhum dos quais são diagnósticos e sendo na maioria dos casos assintomáticos, mas tendo como sintomas a hiperreflexia persistente, dor de cabeça, confusão, dor abdominal, náuseas e vômitos. O diagnóstico incorreto e o reconhecimento tardio da síndrome HELLP são comuns devido à apresentação vaga e variável. Quando a síndrome HELLP é identificada, o parto é necessário para evitar desfechos maternos e neonatais catastróficos. A identificação dos parâmetros basais preditivos da evolução da doença é, portanto, de grande importância para definir qual abordagem obstétrica deve ser priorizada.
O uso de plantas medicinais no âmbito da promoção da saúde no Brasil: uma revisão integrativa
Authors: Heringer Tiago Antonio , Vilichane Ivinildo José , Garcia Edna Linhares , Krug Suzane Beatriz Frantz , Possuelo Lia Gonçalves
Published: November 2021

As plantas medicinais são uma das primeiras formas de medicamento conhecido na humanidade através de chás, e atualmente ainda são utilizadas como uma forma de promover saúde e bem-estar. Apesar de fazer parte dos programas governamentais, as plantas medicinais e fitoterápicos ainda não são indicados com frequência. Diante desta realidade, buscou-se identificar o uso de plantas medicinais como fator na promoção de saúde, realizando uma revisão integrativa, incluindo-se as bases de dados PUBMED, LILACS e SCOPUS. Foram utilizados os descritores “Plantas Medicinais e Promoção da Saúde”, para seleção dos artigos publicados de 2001 a 2021. Foram selecionados 22 artigos para análise. O conhecimento sobre o uso das plantas medicinais é passado de geração em geração no ambiente familiar, e os estudos demonstram que ainda existe uma barreira entre o conhecimento acadêmico e o saber popular, evidenciado pelas pesquisas que tratam da visão dos profissionais sobre o encorajamento ao uso das mesmas. Há uma tendência na melhora de pacientes com o uso das plantas medicinais no que tange o aspecto de qualidade de vida e bem estar, sendo as rodas de conversa e hortas terapêuticas as principais formas de integração entre promoção de saúde e plantas medicinais e as melhores maneiras de diminuição dessa distância de saberes.
Rastreamento do câncer de colo de útero no pré-natal
Authors: Nepomuceno Isadora Fernanda Campos , Moreira Anna Clara Araújo , Souza Laís Rodrigues , Silva Luísa Giarola , Borges Mírian de Sousa , Andrade Silmara Nunes de
Published: November 2021

Tendo em vista que o câncer de colo de útero é uma doença que pode acometer gestantes na mesma proporção de não gestantes, este estudo teve como objetivo identificar os fatores que dificultam a adesão das gestantes à realização do exame durante o acompanhamento pré-natal, visto que este é o momento mais oportuno para a realização do mesmo. Foi realizada uma revisão integrativa de literatura, utilizando artigos disponíveis online na Biblioteca Virtual de Saúde (BVS) e na National Library of Medicne (PubMed) publicados nos últimos 5 anos, sendo excluídos teses, dissertações, artigos duplicados e que não se adequavam ao tema proposto. Foram selecionados para integrar este estudo 04 artigos da base de dados citada anteriormente. Foi realizado a leitura na íntegra dos artigos selecionados para a construção do trabalho, a fim de identificar os fatores que dificultam à adesão. Houve a possibilidade de identificar como barreira o baixo índice de conhecimento das gestantes, visto que a maioria acredita que a realização do exame durante a gestação pode ser prejudicial ao bebê ou não conhecem acerca do que o mesmo previne, acreditando ser desnecessária sua realização. Outros fatores dificultadores foram identificados, demonstrando que a maioria das mulheres não se adequam à realização do exame durante o pré-natal. Assim, ressalta-se a necessidade de maiores ações educativas que auxiliem as mulheres no esclarecimento acerca da importância de realizar o exame no decorrer do período gestacional, beneficiando-as no que diz respeito à prevenção e/ou detecção precoce de qualquer anormalidade.
Guarda compartilhada: princípio do melhor interesse da criança e do adolescente como norteador das decisões de guarda
Authors: Reis Hilderlane Santana , Alves Antonio Sousa
Published: November 2021

Este trabalho visa estudar a guarda compartilhada frente ao princípio do melhor interesse da criança e do adolescente à luz da legislação e jurisprudência. Através de um estudo sobre o poder familiar e da guarda compartilhada em si. Ressalta-se as inúmeras consequências da quebra do vínculo matrimonial, sobretudo na vida dos filhos. Com a evolução global nas últimas décadas, revolução sexual e questionamento do fundamentalismo religioso, a guarda dos filhos tornou-se problema crônico da sociedade capitalista. Assim, este trabalho objetiva estudar a relação entre guarda compartilhada e o princípio do melhor interesse da criança e do adolescente, demonstrando importância e efeitos da mesma em relação aos filhos após rompimento do vínculo conjugal. Ante o exposto, evidenciou-se a necessidade de observar as grandes modificações ocorridas na sociedade, sendo elas culturais, socio-econômicas, políticas e de preferência nas famílias e relações entre pais e filhos, destacando-se um grande aumento de rupturas de laços conjugais. Optou-se por uma pesquisa qualitativa de caráter bibliográfico e documental, buscando-se em bases eletrônicas de dados, artigos científicos e revistas, sendo a principal a Scientific Eletronic Library online (ScieLo). Por fim, considerando o princípio do melhor interesse da criança e do adolescente, tem-se que a guarda compartilhada, ao propiciar uma maior convivência familiar, assim como uma eficaz participação de ambos os pais na educação de sua prole, fatores imprescindíveis para preservação do bem-estar emocional dos infantes, revela-se como modelo que leva à efetivação do princípio do melhor interesse da criança e do adolescente.
Edifício sustentável: estudo de caso das moradas infantis de Canuanã em Formoso do Araguaia-TO
Authors: Barros Enicléia , Anjos Pedro Filipe Amaral , Pereira Matheus Henrique Lopes
Published: November 2021

A sociedade está se movendo em direção a novas ideias relacionadas ao consumo dos recursos naturais existentes. Por esse motivo a sustentabilidade tem ganhado bastante espaço no dia a dia na vida da sociedade. Através da construção sustentável o impacto ambiental será diminuído drasticamente. Portanto, este presente artigo demonstra uma proposta de construção sustentável. A engenharia civil produziu impactos ambientais que podem ser minimizados e determinados. Portanto, é necessário buscar uma relação agradável com o meio ambiente, pois o meio ambiente precisa ser equilibrado, de forma a ser benéfico ao desenvolvimento da sociedade atual sem consumir os recursos naturais. A utilização de materiais sustentáveis e a aplicação de princípios sustentáveis na construção têm se mostrado alternativas para o desenvolvimento sustentável na área da construção civil. Este trabalho tem como objetivo analisar um projeto executado de um lar infantil com aplicação da construção sustentável na engenharia civil, que resultou em diversas premiações nacionais e internacionais.
Effects of environmental factors within the spawning area and migration routes on the length of Anguilla japonica glass eels recruited to Taiwan
Authors: Hsiung KM , Ma C , Ko CY , Tseng YH , Kuo YC , Han YS
Published: November 2021

Growth extent varies considerably among recruiting cohorts of Japanese eels Anguilla japonica. However, the effects of oceanic variation on their growth, particularly during the larval stage, remain unclear. We sampled glass eels in northeast Taiwan from 2010 to 2019 and investigated the effects of sea surface temperature (SST), salinity, chlorophyll a (chl a) concentration, and El Niño-Southern Oscillation (ENSO) events on the total length (TL) of these eels by developing a generalized additive model. The results revealed that mean SST between 21 and 24.5°C in the spawning area was associated with TL. Larger TL values were observed from 0.03-0.07 mg m-3 in the spawning area, and along the migration routes TL was greatest when chl a was > 13 mg m-3. Other variables, including mean SST and salinity along the migration route, influenced TL but contributed less than 4.5%. Larger and smaller A. japonica glass eels were observed during El Niño and La Niña years, respectively. Specifically, El Niño years exerted the greatest influence (67.1%) on TL, and this was followed by mean SST (12%) and accumulated chl a concentration (11.4%) within the spawning area. Our results indicated that environmental factors within the spawning area influenced the TL of A. japonica glass eels to a greater extent than did those along the migration routes, and climatic ENSO events exerted an additional important effect regarding changes in TL. Taken together, our results provide fundamental ecological information and a basis for fisheries to more effectively manage A. japonica.
Clear-cut wave height thresholds reveal dominance shifts in assemblage patterns on rocky shores
Authors: Burel T , Schaal G , Grall J , Le Duff M , Ar Gall E
Published: November 2021

Physical constraints, and particularly hydrodynamics, are major drivers of macroalgal canopy-dominated rocky shore communities. The abundance of habitat-forming seaweeds decreases with increasing wave exposure, triggering cascading effects on their associated communities. This study aims at describing the structure of benthic communities along a hydrodynamic gradient and understanding the role of wave height in structuring the ecological transition from macroflora to macrofauna dominance. In situ wave height was measured together with biological communities (macroalgae, macrograzers and sessile fauna) along an exposure gradient on 3 rocky shores of western Brittany. Results showed that Fucales cover and the abundance of several understory organisms are negatively correlated to in situ wave height, except for barnacles and limpets which were positively correlated. Results revealed the occurrence of a wave height threshold at approximately 80 cm from high to mid-shore levels. Beyond this threshold, we observed a large dominance of barnacles, while below this threshold, Fucales and the associated organisms abounded. A similar threshold was observed on low shore habitats, for barnacle cover only. The results of this study challenge the commonly accepted paradigm of a gradual shift from seaweed-dominated to sessile fauna-dominated zones with increasing wave exposure on rocky shores. Finally, this study sheds new light onto interspecific interactions which drive the structure of rocky shore-associated communities.
SYSTEM CONCEPT OF ENERGY- RESOURCE SYNERGY AND METHODOLOGY OF TECHNOLOGICAL-INNOVATIVE MANAGEMENT ON MOTOR TRANSPORT
Authors:   National Transport University, Kyiv, Ukraine, R Khabutdinov
Published: November 2021

The article discusses the concept of energy-resource synergy in motor transport and in the motor transport system, as well as the main provisions of the methodology of technological innovation management in motor transport. Proposed: a new epistemological paradigm of the motor transport as a sphere of material production; technologically innovative concept of energy-resource synergy in the transport system; a set of methods for technologically innovative management of energy - resource quality of transport services and for resource-synergistic improvement of the functions of the transport system and the subsystem "Motor transport"; analytical model of the target function of strategic management in motor transport. A single energy- resource mechanism for the functioning of motor transport and the material integrity of the motor transport system has been established. The ways of innovative resource saving on transport and in infrastructure subsystems are revealed. The object of study is the structural and functional organization of motor transport and motor transport system. The purpose of the work is the formation of a system-synergistic target function of project management at highly technological and intensively resource-saving transport services based on the innovative requirements and the concept of energy-resource synergy in the transport system. The research method is a theoretical analysis and synthesis of the conceptual target function of the technology and innovation management in transport, taking into account the concept of energy-resource synergy in the transport system. The results of the article can be used to implement system-synergistic and conceptual-innovative management in transport and in other subsystems. Predicted assumptions about the development of the object of study are the implementation of a technologically innovative mechanism of energy-resource synergy in the motor transport and in the motor transport system, taking into account their structures and functions. KEYWORDS: CONCEPT, TECHNOLOGICAL INNOVATIONS, MOTOR TRANSPORT, SYSTEM, FUNCTIONS, RESOURCES, ENERGY-RESOURCE SYNERGY, LIFE CYCLE, QUALITY OF TRANSPORT SERVICES, MANAGEMENT.
The Effect Of Online Learning On Academic Interest Of Evangelical Teological Seminary Of Indonesia - Surabaya
Authors: Boka Christian Melkianus , Setyarini Lilis , Koeswardoyo Willopo
Published: November 2021

Interest in learning is an interest in a lesson and encourages individuals to learn and pursue the lesson, Interest in learning is an actualized desire or intention to be involved in certain learning activities while online learning is a learning process with the help of information and communication technology which is done online or virtual with not face to face. The online learning process carried out during the COVID-19 pandemic has had a major impact on education. This study aims to determine whether there is an effect of online learning on student interest in Evangelical Theological Seminary of Indonesia - Surabaya. The research method used in this research is a quantitative research design with a survey method. Characteristics of respondents from 166 respondents who were the student population of Evangelical Theological Seminary of Indonesia – Surabaya studied consisted of age, education, and domicile. The contribution of the online learning variable in shaping the student's learning interest variable at Evangelical Theological Seminary of Indonesia – Surabaya is 55.6%, the researcher argues that online learning is effective for continuous use and becomes a learning system that complements the traditional learning process.
Power and Positivity: Psycholinguistic Perspectives on Word Valence in Canadian Parliament
Authors: Gallant Jordan , Libben Gary
Published: November 2021

Politicians are skilled language users who deploy words strategically and pay close attention to the emotions that those words evoke. We examined the emotional characteristics of over 92 million words spoken by Canadian Members of Parliament between 2006 and 2021. The analysis brought together the Warriner, Kuperman, and Brysbaert (Behav. Res., 2013, 45, 1191–1207) database of valence (positivity) ratings for English and the Canadian Hansard, which contains a transcription of parliamentary speech. Results revealed that the positivity of words used by politicians in parliament was significantly related to both political and social variables. Politicians increased the positivity of their language after the onset of the COVID-19 crisis. Within the time of the crisis, word positivity was linked statistically to month-by-month case counts, indicating a very fine-grained sensitivity to social realities. Our analysis also revealed a fine-grained sensitivity of word valence to political realities. As expected, parties in power used more positive language than those in opposition. In addition, our analysis revealed that individual parties have characteristic levels of word positivity and that those levels change in accordance with political changes as specific as whether or not the party in power holds a majority of seats in parliament. These findings suggest that the emotional properties of words used by Members of Parliament are reliably indexed to sociopolitical dynamics. The findings also suggest that the methodology of linking individual word ratings to Hansard Documents (which are used to document Parliamentary activities in over 25 countries) can provide a key tool for the understanding of specific crises such as the COVID-19 global pandemic as well as more general social and political trends across countries and languages.
Six-Month Effectiveness of Advanced vs. Standard Hybrid Closed-Loop System in Children and Adolescents With Type 1 Diabetes Mellitus
Authors: Tornese Gianluca , Buzzurro Francesca , Carletti Claudia , Faleschini Elena , Barbi Egidio
Published: November 2021

Introduction

The purpose of this study was to assess the effectiveness of advanced- (a-HCL) vs. standard-hybrid closed-loop (s-HCL) systems use up to 6 months of treatment in a real-world setting of children and adolescents with T1DM.

Methods

We retrospectively evaluated all T1DM pediatric users of MiniMed™ 670G system (s-HCL) and 780G system (a-HCL). HbA1c and BMI were collected at baseline and three and six months after HCL start. Data on glycemic control were extracted from reports generated with CareLink™ Personal Software in Manual Mode, at HCL start, after one, three, and six months after HCL beginning.

Results

The study included 44 individuals with a median age of 13.3 years (range 2- 21 years), 20 on s-HCL, and 24 on a-HCL. a-HCL users had a significantly lower HbA1c compared to s-HCL after six months of HCL use (7.1 vs. 7.7%). Significant differences in HbA1c between a-HCL and s-HCL users were found in children aged 7-14 years (7.1 vs. 7.7% after six months) and in those with a worse (HbA1c >8%) glycemic control at the beginning (7.1 vs. 8.1% after six months). No significant changes in HbA1c were found in a-HCL users that previously used a s-HCL system. Nevertheless, only the use of a-HCL significantly predicted a lower HbA1c after six months. All sensor-specific measures of glycemic control improved from Manual to Auto mode, in both s-HCL and a-HCL, without increasing time spent in hypoglycemia. However, the percentage of individuals with TIR>70% increased significantly in a-HCL users, who attained this target earlier and more stably: younger age, a higher rate of auto-correction, and a lower amount of CHO inserted predicted a TIR>70%. BMI SDS did not significantly change throughout the study period.

Conclusion

This real-world study suggests that effectiveness might be greater in a-HCL than in s-HCL, with significant changes in HbA1c, and reaching earlier and more stably the target of TIR >70%, without increasing hypoglycemia or BMI. At the same time, previous users of s-HCL systems did not show any further improvement with a-HCL. Children under the age of 14 years of age, not represented in previous studies, seem to benefit the most from a-HCL pumps as well as individuals with the worst glycemic control.
Gluten-free Diet Reduces the Risk of Irritable Bowel Syndrome: A Mendelian Randomization Analysis
Authors: Sun Yuhao , Chen Xuejie , Wang Shuyang , Deng Minzi , Xie Ying , Wang Xiaoyan , Chen Jie , Hesketh Therese
Published: November 2021

Background: Whether a gluten-free diet (GFD) is a cause of irritable bowel syndrome (IBS) remains controversial. We aim at exploring the causal relationship between gluten intake and IBS within Mendelian randomization (MR) design.

Methods: We conducted a two-sample MR and selected single-nucleotide polymorphisms (SNPs) associated with GFD as instrumental variables (IVs). SNPs and genetic associations with GFD and IBS were obtained from the latest genome-wide association studies (GWAS) in Europeans (GFD: cases: 1,376; controls: 63,573; IBS: cases:1,121; controls: 360,073). We performed inverse variance weighting (IVW) as the primary method with several sensitivity analyses like MR-Egger and MR-PRESSO for quality control. The above analyses were re-run using another large dataset of IBS, as well as changing the p-value threshold when screening IVs, to verify the stability of the results.

Results: The final estimate indicated significant causal association [per one copy of effect allele predicted log odds ratio (OR) change in GFD intake: OR = 0.97, 95% confidence interval (CI) 0.96 to 0.99, p < 0.01] without heterogeneity statistically (Q = 2.48, p = 0.78) nor horizontal pleiotropy biasing the causality (p = 0.92). Consistent results were found in validation analyses. Results of MR Steiger directionality test indicated the accuracy of our estimate of the causal direction (Steiger p < 0.001).

Conclusion: GFD might be a protective factor of IBS. Therefore, we suggest taking a diet of lower gluten intake into account in IBS prevention and clinical practice.
Role of Flavonoids as Epigenetic Modulators in Cancer Prevention and Therapy
Authors: Fatima Nishat , Baqri Syed Shabihe Raza , Bhattacharya Atrayee , Koney Nii Koney-Kwaku , Husain Kazim , Abbas Ata , Ansari Rais A.
Published: November 2021

Epigenetic regulation involves reversible changes in histones and DNA modifications that can be inherited without any changes in the DNA sequence. Dysregulation of normal epigenetic processes can lead to aberrant gene expression as observed in many diseases, notably cancer. Recent insights into the mechanisms of DNA methylation, histone modifications, and non-coding RNAs involved in altered gene expression profiles of tumor cells have caused a paradigm shift in the diagnostic and therapeutic approaches towards cancer. There has been a surge in search for compounds that could modulate the altered epigenetic landscape of tumor cells, and to exploit their therapeutic potential against cancers. Flavonoids are naturally occurring phenol compounds which are abundantly found among phytochemicals and have potentials to modulate epigenetic processes. Knowledge of the precise flavonoid-mediated epigenetic alterations is needed for the development of epigenetics drugs and combinatorial therapeutic approaches against cancers. This review is aimed to comprehensively explore the epigenetic modulations of flavonoids and their anti-tumor activities.
The Circadian Clock Protein BMAL1 Acts as a Metabolic Sensor In Macrophages to Control the Production of Pro IL-1β
Authors: Timmons George A. , Carroll Richard G. , O’Siorain James R. , Cervantes-Silva Mariana P. , Fagan Lauren E. , Cox Shannon L. , Palsson-McDermott Eva , Finlay David K. , Vincent Emma E. , Jones Nicholas , Curtis Annie M.
Published: November 2021

The transcription factor BMAL1 is a clock protein that generates daily or circadian rhythms in physiological functions including the inflammatory response of macrophages. Intracellular metabolic pathways direct the macrophage inflammatory response, however whether the clock is impacting intracellular metabolism to direct this response is unclear. Specific metabolic reprogramming of macrophages controls the production of the potent pro-inflammatory cytokine IL-1β. We now describe that the macrophage molecular clock, through Bmal1, regulates the uptake of glucose, its flux through glycolysis and the Krebs cycle, including the production of the metabolite succinate to drive Il-1β production. We further demonstrate that BMAL1 modulates the level and localisation of the glycolytic enzyme PKM2, which in turn activates STAT3 to further drive Il-1β mRNA expression. Overall, this work demonstrates that BMAL1 is a key metabolic sensor in macrophages, and its deficiency leads to a metabolic shift of enhanced glycolysis and mitochondrial respiration, leading to a heightened pro-inflammatory state. These data provide insight into the control of macrophage driven inflammation by the molecular clock, and the potential for time-based therapeutics against a range of chronic inflammatory diseases.
Similarity and Potential Relation Between Periimplantitis and Rheumatoid Arthritis on Transcriptomic Level: Results of a Bioinformatics Study
Authors: Li Shiyi , Zhou Changqing , Xu Yongqian , Wang Yujia , Li Lijiao , Pelekos George , Ziebolz Dirk , Schmalz Gerhard , Qin Zeman
Published: November 2021

Background

This bioinformatics study aimed to reveal potential cross-talk genes, related pathways, and transcription factors between periimplantitis and rheumatoid arthritis (RA).

Methods

The datasets GSE33774 (seven periimplantitis and eight control samples) and GSE106090 (six periimplantitis and six control samples) were included from the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO). A differential expression analysis (p < 0.05 and |logFC (fold change)| ≥ 1) and a functional enrichment analysis (p < 0.05) were performed. Based on this, a protein–protein interaction (PPI) network was constructed by Cytoscape. RA-related genes were extracted from DisGeNET database, and an overlap between periimplantitis-related genes and these RA-related genes was examined to identify potential cross-talk genes. Gene expression was merged between two datasets, and feature selection was performed by Recursive Feature Elimination (RFE) algorithm. For the feature selection cross-talk genes, support vector machine (SVM) models were constructed. The expression of these feature genes was determined from GSE93272 for RA. Finally, a network including cross-talk genes, related pathways, and transcription factors was constructed.

Results

Periimplantitis datasets included 138 common differentially expressed genes (DEGs) including 101 up- and 37 downregulated DEGs. The PPI interwork of periimplantitis comprised 1,818 nodes and 2,517 edges. The RFE method selected six features, i.e., MERTK, CD14, MAPT, CCR1, C3AR1, and FCGR2B, which had the highest prediction. Out of these feature genes, CD14 and FCGR2B were most highly expressed in periimplantitis and RA. The final activated pathway–gene network contained 181 nodes and 360 edges. Nuclear factor (NF) kappa B signaling pathway and osteoclast differentiation were identified as potentially relevant pathways.

Conclusions

This current study revealed FCGR2B and CD14 as the most relevant potential cross-talk genes between RA and periimplantitis, which suggests a similarity between RA and periimplantitis and can serve as a theoretical basis for future research.
Might Routine Vitamin A Monitoring in Cystic Fibrosis Patients Reduce Virus-Mediated Lung Pathology?
Authors: Sealy Robert E. , Surman Sherri L. , Vogel Peter , Hurwitz Julia L.
Published: November 2021

Cystic fibrosis (CF) is an autosomal recessive gene disorder that affects tens of thousands of patients worldwide. Individuals with CF often succumb to progressive lung disease and respiratory failure following recurrent infections with bacteria. Viral infections can also damage the lungs and heighten the CF patient’s susceptibility to bacterial infections and long-term sequelae. Vitamin A is a key nutrient important for immune health and epithelial cell integrity, but there is currently no consensus as to whether vitamin A should be monitored in CF patients. Here we evaluate previous literature and present results from a CF mouse model, showing that oral vitamin A supplements significantly reduce lung lesions that would otherwise persist for 5-6 weeks post-virus exposure. Based on these results, we encourage continued research and suggest that programs for the routine monitoring and regulation of vitamin A levels may help reduce virus-induced lung pathology in CF patients.
Pathogenesis of IgA Vasculitis: An Up-To-Date Review
Authors: Song Yan , Huang Xiaohan , Yu Guizhen , Qiao Jianjun , Cheng Jun , Wu Jianyong , Chen Jianghua
Published: November 2021

Immunoglobin A (IgA) vasculitis (IgAV), formerly called the Henoch-Schönlein purpura (HSP), is a small vessel vasculitis, characterized by IgA1-dominant immune deposition at diseased vessel walls. IgAV is the most common form of vasculitis in children; typical symptoms include palpable purpura, arthritis or arthralgia, abdominal pain, and hematuria or proteinuria. Galactose-deficient IgA1 is detected in the tissues of the kidney and skin in patients with IgAV; it forms immune complexes leading to subsequent immune reactions and injuries. This report provides the recent advances in the understanding of environmental factors, genetics, abnormal innate and acquired immunity, and the role of galactose-deficient IgA1 immunocomplexes in the pathogenesis of IgAV.
An Integrated, Size-Structured Stock Assessment of Antarctic Krill, Euphausia superba
Authors: Wang Rui , Song Puqing , Li Yuan , Lin Longshan
Published: November 2021

The Antarctic krill (Euphausia superba) fishery is the largest fishery in the Southern Ocean, and it has been operating for over 45 years. In the past decade, the spatial distribution of the krill fishery has focused on the Bransfield Strait off the Antarctic Peninsula (subarea 48.1). However, the high fishing effort and climate change have placed great pressure on krill resources in this region, and conservation concerns have been raised. Because aging krill is difficult and uncertain, we developed an integrated size-structured model to estimate the fishing mortality, recruitment and spawning biomass of krill. The results indicated that in 1992–2011, the average spawning biomass of krill ranged from 1.14 × 106 to 1.45 × 106 tons, the estimated biomass of the maximum sustainable yield (BMSY) ranged from 3.96 × 105 to 4.90 × 105 tons and the estimated average recruitment ranged from 2.3 × 1012 to 5.03 × 1012 individuals in the research area. We explored the effect of different data weighting schemes for the length data on the assessment output. However, our estimates also have uncertainties. In addition, an attempt was made to analyze the correlation between model-estimated recruitment and mature biomass with climate change. Future stock assessments of krill resources should be comprehensive and multimethod, and the management of krill resources should be based on science that considers the demand of fishing communities and ecosystem protection.
Chromosome Genome Sequencing and Comparative Transcriptome-Based Analyses of Kloeckera apiculata 34-9 Unveil the Potential Biocontrol Mechanisms Against Citrus Green Mold
Authors: Tian Zhonghuan , Du Yujie , Yang Fan , Zhao Juan , Liu Shuqi , Zhang Deyao , Long Chao-an
Published: November 2021

Biological control is an environmentally friendly, safe, and replaceable strategy for disease management. Genome sequences of a certain biocontrol agent could lay a solid foundation for the research of molecular biology, and the more refined the reference genome, the more information it provides. In the present study, a higher resolution genome of Kloeckera apiculata 34-9 was assembled using high-throughput chromosome conformation capture (Hi-C) technology. A total of 8.07 M sequences of K. apiculata 34-9 genome was anchored onto 7 pesudochromosomes, which accounting for about 99.51% of the whole assembled sequences, and 4,014 protein-coding genes were annotated. Meanwhile, the detailed gene expression changes of K. apiculata 34-9 were obtained under low temperature and co-incubation with Penicillium digitatum treatments, respectively. Totally 254 differentially expressed genes (DEGs) were detected with low temperature treatment, of which 184 and 70 genes were upregulated and downregulated, respectively. Some candidate genes were significantly enriched in ribosome biosynthesis in eukaryotes and ABC transporters. The expression of gene Kap003732 and Kap001595 remained upregulated and downregulated through the entire time-points, respectively, indicating that they might be core genes for positive and negative response to low temperature stress. When co-incubation with P. digitatum, a total of 2,364 DEGs were found, and there were 1,247 upregulated and 1,117 downregulated genes, respectively. Biosynthesis of lysine and arginine, and phenylalanine metabolism were the highest enrichment of the cluster and KEGG analyses of the co-DEGs, the results showed that they might be involved in the positive regulation of K. apiculata 34-9 response to P. digitatum. The completeness of K. apiculata 34-9 genome and the transcriptome data presented here are essential for providing a high-quality genomic resource and it might serve as valuable molecular properties for further studies on yeast genome, expression pattern of biocontrol system, and postharvest citrus storage and preservation.
Risk Stratification for Organ/Space Surgical Site Infection in Advanced Digestive System Cancer
Authors: Sun Chen , Gao Hui , Zhang Yuelun , Pei Lijian , Huang Yuguang
Published: November 2021

Background

Organ/space surgical site infection (organ/space SSI) is a serious postoperative complication, closely related to a poor prognosis. Few studies have attempted to stratify the risk of organ/space SSI for patients with advanced digestive system cancer. This study aimed to identify a simple risk stratification for these patients based on perioperative factors.

Methods

The study was based on two randomized controlled trials (RCT) (NCT02715076, ChiCTR-IPR-17011099), including 839 patients undergoing elective radical resection of advanced digestive system cancer. The primary outcome was organ/space SSI within 30 days after surgery. Multivariable logistic regression model was used to identify risk factors. The risk of organ/space SSI stratified over those risk factors was compared using chi-square tests and the relative risk (RR) was estimated.

Results

Among the 839 patients, 51 developed organ/space SSI (6.1%) within 30 days after surgery. According to the multivariable logistic regression model, 3 procedure types, including gastrectomy (OR=8.22, 95% CI: 2.71-24.87, P<0.001), colorectal resection (OR=8.65, 95% CI: 3.13-23.85, P<0.001) and pancreatoduodenectomy (OR=7.72, 95% CI: 2.95-20.21, P<0.001), as well as anaesthesia time > 4 h (OR=2.38, 95% CI: 1.08-5.27, P=0.032) and prolonged ICU stay (OR=4.10, 95% CI: 1.67-10.10, P=0.002), were risk factors for postoperative organ/space SSI. The number of risk factors was significantly associated with an increased risk of organ/space SSI (P<0.001), which was 2.8% in patients with 0-1 risk factor (RR=0.20, 95% CI: 0.11-0.35), 13.0% in patients with 2 risk factors (RR=3.64, 95% CI: 2.14-6.20) and 35.7% in patients with 3 risk factors (RR=6.41, 95% CI: 3.01-13.65).

Conclusion

This study is a preliminary exploratory and provides a simple risk stratification to identify the risk of postoperative organ/space SSI for patients with advanced digestive system cancer. Further research is needed to validate and generalize the results in a wider population.

Clinical Trial Registration

ClinicalTrials.gov, identifier NCT02715076; Chinese Clinical Trial Registry [https://www.chictr.org.cn/enindex.aspx], identifier ChiCTR-IPR-17011099.
Red Clover HDT, a BAHD Hydroxycinnamoyl-Coenzyme A:L-3,4-Dihydroxyphenylalanine (L-DOPA) Hydroxycinnamoyl Transferase That Synthesizes Clovamide and Other N-Hydroxycinnamoyl-Aromatic Amino Acid Amides
Authors: Sullivan Michael L. , Knollenberg Benjamin J.
Published: November 2021

Red clover leaves accumulate high levels (up to 1 to 2% of dry matter) of two caffeic acid derivatives: phaselic acid (2-O-caffeoyl-L-malate) and clovamide [N-caffeoyl-L-3,4-dihydroxyphenylalanine (L-DOPA)]. These likely play roles in protecting the plant from biotic and abiotic stresses but can also help preserve protein during harvest and storage of the forage via oxidation by an endogenous polyphenol oxidase. We previously identified and characterized, a hydroxycinnamoyl-coenzyme A (CoA):malate hydroxycinnamoyl transferase (HMT) from red clover. Here, we identified a hydroxycinnamoyl-CoA:L-DOPA hydroxycinnamoyl transferase (HDT) activity in unexpanded red clover leaves. Silencing of the previously cloned HMT gene reduced both HMT and HDT activities in red clover, even though the HMT enzyme lacks HDT activity. A combination of PCR with degenerate primers based on BAHD hydroxycinnamoyl-CoA transferase sequences and 5′ and 3′ rapid amplification of cDNA ends was used to clone two nearly identical cDNAs from red clover. When expressed in Escherichia coli, the encoded proteins were capable of transferring hydroxycinnamic acids (p-coumaric, caffeic, or ferulic) from the corresponding CoA thioesters to the aromatic amino acids L-Phe, L-Tyr, L-DOPA, or L-Trp. Kinetic parameters for these substrates were determined. Stable expression of HDT in transgenic alfalfa resulted in foliar accumulation of p-coumaroyl- and feruloyl-L-Tyr that are not normally present in alfalfa, but not derivatives containing caffeoyl or L-DOPA moieties. Transient expression of HDT in Nicotiana benthamiana resulted in the production of caffeoyl-L-Tyr, but not clovamide. Coexpression of HDT with a tyrosine hydroxylase resulted in clovamide accumulation, indicating the host species’ pool of available amino acid (and hydroxycinnamoyl-CoA) substrates likely plays a major role in determining HDT product accumulation in planta. Finally, that HDT and HMT proteins share a high degree of identity (72%), but differ substantially in substrate specificity, is promising for further investigation of structure-function relationships of this class of enzymes, which could allow the rational design of BAHD enzymes with specific and desirable activities.
Explicating Brand Equity in the Information Technology Sector in Vietnam
Authors: Huynh Hien Thi Ngoc , Trieu Hoa Doan Xuan , Nguyen Phuong Van , Tran Tue Gia , Lam Long Nguyen Hai
Published: November 2021

Vietnamese IT businesses have expanded internationally and have reached a turning point with opportunities and challenges typical when establishing a global brand. To assist Vietnamese IT firms in the development of branding strategies, this study investigated the direct influences of various firm competencies, such as innovation, marketing, networking, and dynamic capabilities, on brand equity in the business-to-business (B2B) information technology (IT) industry. In addition, the study examined whether the enterprise’s capabilities indirectly affected B2B brand equity via value co-creation and customer value. By employing PLS-SEM to analyze a sample of 182 questionnaire responses from IT firms in Ho Chi Minh City, Vietnam, the study found that innovative, networking, and dynamic capabilities had a direct, positive effect on brand equity. The mediating effect of value co-creation and customer value on the association between marketing capability and brand equity was also reported. The research also recommended branding strategies for enterprises that seek to improve their internal competencies and abilities to innovate and respond swiftly to market dynamics. In a successful marketing strategy, all stakeholders must be involved in the value co-creation and brand equity building process, and this is particularly critical in a knowledge-intensive industry such as IT.
A Preliminary Study on the Relationship between Parasitaemia and Cytokine Expression of Peripheral Blood Cells in Trypanosoma vivax-Experimentally Infected Cattle
Authors: Fidelis Junior Otavio Luiz , Sampaio Paulo Henrique , Gonçalves Luiz Ricardo , Machado Rosangela Zacarias , André Marcos Rogério , Wijffels Gene , Cadioli Fabiano Antonio
Published: November 2021

Trypanosoma vivax outbreaks have been reported with increasing frequency worldwide, causing significant economic losses in livestock. Though several studies have suggested that cytokine responses may influence infection caused by Trypanosoma sp., their exact role remains unclear and may vary according to the animal species and parasite strain. The present study aimed to evaluate cytokine expression of peripheral blood cells from three Girolando dairy cows experimentally infected with T. vivax. For this purpose, blood samples were collected prior to the inoculation on the day of inoculation (D0), the day after inoculation (D1), and then every seven days up to 119 days after infection (DAI). Each animal presented a unique pattern of cytokine expression. While a tendency of a Th1 cytokine response was observed during the patent phase (presence of circulating parasites), an increase of Th2 cytokine expression was found at the beginning of the sub-patent phase (low parasitaemia or aparasitaemic periods). In animals that presented a better control of parasitaemia, IL-6 and IFNγ increased during most of the trial period. On the other hand, the cow that presented reduction of IL-1β, IL-2, and TNFα during the entire period did not control parasitaemia properly. A balance between the Th1 and Th2 profile is beneficial for parasite control and animal health. The results found in the present study are a first step towards elucidating the dynamics of cattle’s inflammatory response against T. vivax, requiring future studies focusing on the role of key cytokines on the controlling of parasitaemia in different stages of bovine trypanosomosis.
Addition of Different Levels of Humic Substances Extracted from Worm Compost in Broiler Feeds
Authors: Domínguez-Negrete Alejandra , Gómez-Rosales Sergio , Angeles María de Lourdes , López-Hernández Luis Humberto , Reis de Souza Tercia Cesaria , Latorre-Cárdenas Juan David , Téllez-Isaias Guillermo
Published: November 2021

Different sources and inclusion levels of humic substances (HS) have been tested in broiler rations as an alternative to the addition of growth promoter antibiotics (GPA) with promising results. The current study was carried out to assess the influence of HS extracted from worm compost on broiler production parameters, carcass yield, tibia characteristics, lactic acid bacteria (LAB) counts, excretion of Eimeria oocysts, and antioxidant status of breast meat. A total of 1200 broilers were used, housed in groups of 30 per pen, and assigned to five treatments: 1 = basal diet with GPA (positive control), 2 = basal diet without GPA (negative control), 3–5 = basal diet with 0.15, 0.30, and 0.45% HS, respectively. The data was subjected to a variance analysis and orthogonal contrasts. The FI decreased linearly (p < 0.05) from 1–14, 29–42, and 1–42 days as the inclusion of HS in the feed increased. The FCR had quadratic responses (p < 0.01) from 29–42 and 1–42 days concerning the HS inclusion levels. Lactic acid bacteria was higher (p ˂ 0.05) in ten-day-old chicks with 0.45% HS in the diet. The 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity antioxidant potential decreased linearly (p < 0.05) concerning increasing HS in the feed. The results indicate that HS can be used as growth promoters in broiler feeds.
A Novel Method to Assess Motor Cortex Connectivity and Event Related Desynchronization Based on Mass Models
Authors: Ursino Mauro , Ricci Giulia , Astolfi Laura , Pichiorri Floriana , Petti Manuela , Magosso Elisa
Published: November 2021

Knowledge of motor cortex connectivity is of great value in cognitive neuroscience, in order to provide a better understanding of motor organization and its alterations in pathological conditions. Traditional methods provide connectivity estimations which may vary depending on the task. This work aims to propose a new method for motor connectivity assessment based on the hypothesis of a task-independent connectivity network, assuming nonlinear behavior. The model considers six cortical regions of interest (ROIs) involved in hand movement. The dynamics of each region is simulated using a neural mass model, which reproduces the oscillatory activity through the interaction among four neural populations. Parameters of the model have been assigned to simulate both power spectral densities and coherences of a patient with left-hemisphere stroke during resting condition, movement of the affected, and movement of the unaffected hand. The presented model can simulate the three conditions using a single set of connectivity parameters, assuming that only inputs to the ROIs change from one condition to the other. The proposed procedure represents an innovative method to assess a brain circuit, which does not rely on a task-dependent connectivity network and allows brain rhythms and desynchronization to be assessed on a quantitative basis.
Molecular Progression of Myeloproliferative and Myelodysplastic/Myeloproliferative Neoplasms: A Study on Sequential Bone Marrow Biopsies
Authors: Brune Magdalena M. , Rau Achim , Overkamp Mathis , Flaadt Tim , Bonzheim Irina , Schürch Christian M. , Federmann Birgit , Dirnhofer Stefan , Fend Falko , Tzankov Alexandar
Published: November 2021

Myeloproliferative neoplasms (MPN) and myelodysplastic/myeloproliferative neoplasms (MDS/MPN) both harbor the potential to undergo myelodysplastic progression or acceleration and can transform into blast-phase MPN or MDS/MPN, a form of secondary acute myeloid leukemia (AML). Although the initiating transforming events are yet to be determined, current concepts suggest a stepwise acquisition of (additional) somatic mutations—apart from the initial driver mutations—that trigger disease evolution. In this study we molecularly analyzed paired bone marrow samples of MPN and MDS/MPN patients with known progression and compared them to a control cohort of patients with stable disease course. Cases with progression displayed from the very beginning a higher number of mutations compared to stable ones, of which mutations in five (ASXL1, DNMT3A, NRAS, SRSF2 and TP53) strongly correlated with progression and/or transformation, even if only one of these genes was mutated, and this particularly applied to MPN. TET2 mutations were found to have a higher allelic frequency than the putative driver mutation in three progressing cases (“TET2-first”), whereas two stable cases displayed a TET2-positive subclone (“TET2-second”), supporting the hypothesis that not only the sum of mutations but also their order of appearance matters in the course of disease. Our data emphasize the importance of genetic testing in MPN and MDS/MPN patients in terms of risk stratification and identification of imminent disease progression.