bluuebunny/crossref_metadata_2025 huggingface dataset:
Temperature-sensitive migration dynamics in neutrophil-differentiated HL-60 cells
Authors: Khachaturyan Galina , Holle Andrew W. , Ende Karen , Frey Christoph , Schwederski Heiko A. , Eiseler Tim , Paschke Stephan , Micoulet Alexandre , Spatz Joachim P. , Kemkemer Ralf
Published: April 2022

Cell migration plays an essential role in wound healing and inflammatory processes inside the human body. Peripheral blood neutrophils, a type of polymorphonuclear leukocyte (PMN), are the first cells to be activated during inflammation and subsequently migrate toward an injured tissue or infection site. This response is dependent on both biochemical signaling and the extracellular environment, one aspect of which includes increased temperature in the tissues surrounding the inflammation site. In our study, we analyzed temperature-dependent neutrophil migration using differentiated HL-60 cells. The migration speed of differentiated HL-60 cells was found to correlate positively with temperature from 30 to 42 °C, with higher temperatures inducing a concomitant increase in cell detachment. The migration persistence time of differentiated HL-60 cells was higher at lower temperatures (30–33 °C), while the migration persistence length stayed constant throughout the temperature range. Coupled with the increased speed observed at high temperatures, this suggests that neutrophils are primed to migrate more effectively at the elevated temperatures characteristic of inflammation. Temperature gradients exist on both cell and tissue scales. Taking this into consideration, we also investigated the ability of differentiated HL-60 cells to sense and react to the presence of temperature gradients, a process known as thermotaxis. Using a two-dimensional temperature gradient chamber with a range of 27–43 °C, we observed a migration bias parallel to the gradient, resulting in both positive and negative thermotaxis. To better mimic the extracellular matrix (ECM) environment in vivo, a three-dimensional collagen temperature gradient chamber was constructed, allowing observation of biased neutrophil-like differentiated HL-60 migration toward the heat source.
Prognosis of patients with operated chronic subdural hematoma
Authors: Posti Jussi P. , Luoto Teemu M. , Sipilä Jussi O. T. , Rautava Päivi , Kytö Ville
Published: April 2022

Chronic subdural hematoma (cSDH), previously considered fairly benign and easy to treat, is now viewed a possible sign of incipient clinical decline. We investigated case-fatality, excess fatality and need for reoperations following operated cSDH in a nationwide setting focusing on patient-related characteristics. Finnish nationwide databases were searched for all admissions with operated cSDH as well as later deaths in adults (≥ 16 years) during 2004–2017. There were 8539 patients with an evacuated cSDH (68% men) with a mean age of 73.0 (± 12.8) years. During the follow-up, 3805 (45%) patients died. In-hospital case-fatality was 0.7% (n = 60) and 30-day case-fatality 4.2% (n = 358). The 1-year case-fatality was 14.3% (95% CI = 13.4–15.2%) among men and 15.3% (95% CI = 14.0–16.7%) among women. Comorbidity burden, older age, and alcoholism were significantly associated with fatality. One-year excess fatality rate compared to general Finnish population was 9.1% (95% CI = 8.4–9.9) among men and 10.3% (95% CI = 9.1–11.4) among women. Highest excess fatality was observed in the oldest age group in both genders. Reoperation was needed in 19.4% (n = 1588) of patients. Older age but not comorbidity burden or other patient-related characteristics were associated with increased risk for reoperation. The overall case-fatality and need for reoperations declined during the study era. Comorbidities should be considered when care and follow-up are planned in patients with cSDH. Our findings underpin the perception that the disease is more dangerous than previously thought and causes mortality in all exposed age groups: even a minor burden of comorbidities can be fatal in the post-operative period.
The mediating pathways between parental separation in childhood and offspring hypertension at midlife
Authors: Stannard Sebastian , Berrington Ann , Alwan Nisreen A.
Published: April 2022

Social life course determinants of adult hypertension are relatively unknown. This paper examines how parental separation before age 10 relates to hypertension at age 46. Adjusting for parental confounders and considering the role of adult mediators, we aim to quantify unexplored mediating pathways in childhood using prospectively collected data. Data from the 1970 British Birth Cohort Study are utilised. Hypertension is measured by health care professionals at age 46. Potential mediating pathways in childhood include body mass index (BMI), systolic and diastolic blood pressure, illness, disability, family socioeconomic status (SES) and cognitive and developmental indicators at age 10. Additionally, we explore to what extent childhood mediators operate through adult mediators, including health behaviours, family SES, BMI and mental wellbeing. We also test for effect modification of the relationship between parental separation and hypertension by gender. Nested logistic regression models test the significance of potential mediating variables. Formal mediation analysis utilising Karlson Holm and Breen (KHB) method quantify the direct and indirect effect of parental separation on offspring hypertension at midlife. There was an association between parental separation and hypertension in mid-life in women but not men. For women, family SES and cognitive and behavioural development indicators at age 10 partly mediate the relationship between parental separation and hypertension at age 46. When adult mediators including, health behaviours, family SES, BMI and mental wellbeing are included, the associations between the childhood predictors and adult hypertension are attenuated, suggesting that these childhood mediators in turn may work through adult mediators to affect the risk of hypertension in midlife. We found family SES in childhood, cognitive and behaviour development indicators at age 10, including disruptive behaviour, coordination and locus of control in childhood, to be important mediators of the relationship between parental separation and midlife hypertension suggesting that intervening in childhood may modify adult hypertension risk.
Using de novo genome assembly and high-throughput sequencing to characterize the MHC region in a non-model bird, the Eurasian coot
Authors: Pikus Ewa , Minias Piotr
Published: April 2022

Genes of the Major Histocompatibility Complex (MHC) form a key component of vertebrate adaptive immunity, as they code for molecules which bind antigens of intra- and extracellular pathogens (MHC class I and II, respectively) and present them to T cell receptors. In general, MHC genes are hyper-polymorphic and high MHC diversity is often maintained within natural populations (via balancing selection) and within individuals (via gene duplications). Because of its complex architecture with tandems of duplicated genes, characterization of MHC region in non-model vertebrate species still poses a major challenge. Here, we combined de novo genome assembly and high-throughput sequencing to characterize MHC polymorphism in a rallid bird species, the Eurasian coot Fulica atra. An analysis of genome assembly indicated high duplication rate at MHC-I, which was also supported by targeted sequencing of peptide-binding exons (at least five MHC-I loci genotyped). We found high allelic richness at both MHC-I and MHC-II, although signature of diversifying selection and recombination (gene conversion) was much stronger at MHC-II. Our results indicate that Eurasian coot retains extraordinary polymorphism at both MHC classes (when compared to other non-passerine bird species), although they may be subject to different evolutionary mechanism.
Differences in visual field loss pattern when transitioning from SITA standard to SITA faster
Authors: Le Christopher T. , Fiksel Jacob , Ramulu Pradeep , Yohannan Jithin
Published: April 2022

Swedish Interactive Threshold Algorithm (SITA) Faster is the most recent and fastest testing algorithm for the evaluation of Humphrey visual fields (VF). However, existing evidence suggests that there are some differences in global measures of VF loss in eyes transitioning from SITA Standard to the newer SITA Faster. These differences may be relevant, especially in glaucoma, where VF changes over time influence clinical decisions around treatment. Furthermore, characterization of differences in localizable VF loss patterns between algorithms, rather than global summary measures, can be important for clinician interpretation when transitioning testing strategies. In this study, we determined the effect of transitioning from SITA Standard to SITA Faster on VF loss patterns in glaucomatous eyes undergoing longitudinal VF testing in a real-world clinical setting. Archetypal analysis was used to derive composition weights of 16 clinically relevant VF patterns (i.e., archetypes (AT)) from patient VFs. We found switching from SITA Standard to SITA Faster was associated with less preservation of VF loss (i.e., abnormal AT 2–4, 6–9, 11, 13, 14) relative to successive SITA Standard exams (P value < 0.01) and was associated with relatively greater preservation of AT 1, the normal VF (P value < 0.01). Eyes that transition from SITA Standard to SITA Faster in a real-world clinical setting have an increased likelihood of preserving patterns reflecting a normal VF and lower tendency to preserve patterns reflecting abnormal VF as compared to consecutive SITA Standard exams in the same eye.
New fabrication method for di-indium tri-sulfuric (In2S3) thin films
Authors: Ali Ahmed I. , Ibrahim Medhat , Hassen A.
Published: April 2022

Di-indium tri-sulfuric (In2S3) thin films are fabricated with annealing indium thin films in a sulfur environment. The effect of both annealing temperature and pressure on the structure, morphology, Raman, and photoluminescence (PL) spectroscopy has been studied. The X-ray diffraction (XRD) and field emission scanning electron microscopy (FE-SEM) of the prepared thin films showed different structural phases and morphology with varying annealing temperature and pressure. Energy dispersive X-ray (EDX) analysis confirmed the chemical composition and the atomic ratio of In/S for the In2S3 thin films. The optimum annealing conditions of In2S3 thin films are 550 °C and 100 Torr. The outcome results revealed a new good growth method for In2S3 thin films to be used for different applications.
Arrhythmogenic influence of mutations in a myocyte-based computational model of the pulmonary vein sleeve
Authors: Jæger Karoline Horgmo , Edwards Andrew G. , Giles Wayne R. , Tveito Aslak
Published: April 2022

In the heart, electrophysiological dysregulation arises from defects at many biological levels (from point mutations in ion channel proteins to gross structural abnormalities). These defects disrupt the normal pattern of electrical activation, producing ectopic activity and reentrant arrhythmia. To interrogate mechanisms that link these primary biological defects to macroscopic electrophysiologic dysregulation most prior computational studies have utilized either (i) detailed models of myocyte ion channel dynamics at limited spatial scales, or (ii) homogenized models of action potential conduction that reproduce arrhythmic activity at tissue and organ levels. Here we apply our recent model (EMI), which integrates electrical activation and propagation across these scales, to study human atrial arrhythmias originating in the pulmonary vein (PV) sleeves. These small structures initiate most supraventricular arrhythmias and include pronounced myocyte-to-myocyte heterogeneities in ion channel expression and intercellular coupling. To test EMI’s cell-based architecture in this physiological context we asked whether ion channel mutations known to underlie atrial fibrillation are capable of initiating arrhythmogenic behavior via increased excitability or reentry in a schematic PV sleeve geometry. Our results illustrate that EMI’s improved spatial resolution can directly interrogate how electrophysiological changes at the individual myocyte level manifest in tissue and as arrhythmia in the PV sleeve.
Genetic diversity of hepatitis E virus (HEV) in imported and domestic camels in Saudi Arabia
Authors: El-Kafrawy Sherif A. , Hassan Ahmed M. , El-Daly Mai M. , Al-Hajri Mohammed , Farag Elmoubashar , Elnour Fatimah Ahmed , Khan Anas , Tolah Ahmed M. , Alandijany Thamir A. , Othman Noura A. , Memish Ziad A. , Corman Victor M. , Drosten Christian , Zumla Alimuddin , Azhar Esam I.
Published: April 2022

Camels gained attention since the discovery of MERS-CoV as intermediary hosts for potentially epidemic zoonotic viruses. DcHEV is a novel zoonotic pathogen associated with camel contact. This study aimed to genetically characterize DcHEV in domestic and imported camels in Saudi Arabia. DcHEV was detected by RT-PCR in serum samples, PCR-positive samples were subjected to sequencing and phylogenetic analyses. DcHEV was detected in 1.77% of samples with higher positivity in domestic DCs. All positive imported dromedaries were from Sudan with age declining prevalence. Domestic DcHEV sequences clustered with sequences from Kenya, Somalia, and UAE while imported sequences clustered with one DcHEV isolate from UAE and both sequences clustered away from isolates reported from Pakistan. Full-genome sequences showed 24 amino acid difference with reference sequences. Our results confirm the detection of DcHEV in domestic and imported DCs. Further investigations are needed in human and camel populations to identify DcHEV potential zoonosis threat.
Cell surface detection of vimentin, ACE2 and SARS-CoV-2 Spike proteins reveals selective colocalization at primary cilia
Authors: Lalioti Vasiliki , González-Sanz Silvia , Lois-Bermejo Irene , González-Jiménez Patricia , Viedma-Poyatos Álvaro , Merino Andrea , Pajares María A. , Pérez-Sala Dolores
Published: April 2022

The SARS-CoV-2 Spike protein mediates docking of the virus onto cells prior to viral invasion. Several cellular receptors facilitate SARS-CoV-2 Spike docking at the cell surface, of which ACE2 plays a key role in many cell types. The intermediate filament protein vimentin has been reported to be present at the surface of certain cells and act as a co-receptor for several viruses; furthermore, its potential involvement in interactions with Spike proteins has been proposed. Nevertheless, the potential colocalization of vimentin with Spike and its receptors on the cell surface has not been explored. Here we have assessed the binding of Spike protein constructs to several cell types. Incubation of cells with tagged Spike S or Spike S1 subunit led to discrete dotted patterns at the cell surface, which consistently colocalized with endogenous ACE2, but sparsely with a lipid raft marker. Vimentin immunoreactivity mostly appeared as spots or patches unevenly distributed at the surface of diverse cell types. Of note, vimentin could also be detected in extracellular particles and in the cytoplasm underlying areas of compromised plasma membrane. Interestingly, although overall colocalization of vimentin-positive spots with ACE2 or Spike was moderate, a selective enrichment of the three proteins was detected at elongated structures, positive for acetylated tubulin and ARL13B. These structures, consistent with primary cilia, concentrated Spike binding at the top of the cells. Our results suggest that a vimentin-Spike interaction could occur at selective locations of the cell surface, including ciliated structures, which can act as platforms for SARS-CoV-2 docking.
Photoredox Chemistries of Cyclometalated Ir(iii) Complexes
Authors: You Youngmin
Published: April 2022

Cyclometalated Ir(iii) complexes have emerged as promising alternatives to conventional organic molecules and late transition metal complexes in photoelectronic applications. In particular, photoinduced electron transfer involving Ir(iii) complexes has provided useful principles for creating biosensors and photoredox catalysts. This chapter outlines the general mechanism of inter- and intramolecular photoinduced electron transfer of Ir(iii) complexes. This knowledge is employed to explain the molecular design of phosphorescence probes of biometals and the mechanism of photoredox catalysis of organic transformations. The excited-state electrochemistry demonstrates the unique photofunctionality of the Ir(iii) complexes and will serve as the key principle of novel utilities.
H2O2-activated independently bidirectional regulation for a sensitive and accurate electrochemiluminescence ratiometric analysis
Authors: Liu Xiang-Mei , Wang Yu-Ling , Ren Shu-Wei , Cao Jun-Tao , Liu Yan-Ming
Published: April 2022

A novel ECL ratiometric sensor was developed based on H2O2 activated independently bidirectional regulation strategy.
Ultrasensitive detection of mercury(ii) ions on a hybrid film of a graphene and gold nanoparticle-modified electrode
Authors: Shi Dongmin , Wu Wenzhan , Li Xiaoyuan
Published: April 2022

The cooperation of rGO with AuNPs improves sensing sensitivity due to the large surface area and high electron conductivity. Hg2+ can be detected with high sensitivity due to the specific affinity between Hg and AuNPs.
Enantioselective Pd-catalyzed dearomative reductive Heck and domino Heck–Suzuki reactions of 2-CF3-indoles
Authors: Liang Ren-Xiao , Chen Jian-Fei , Huang Ying-Ying , Yu Ya-Ping , Zhang Han-Yue , Song Yu-Feng , Tsui Gavin Chit , Jia Yi-Xia
Published: April 2022

Highly enantioselective palladium-catalyzed dearomative reductive Heck and domino Heck–Suzuki reactions of 2-CF3-indoles have been developed towards the synthesis of indolines bearing trifluoromethyl quaternary stereocenters.
Palladium-catalyzed carboxylative cyclization of propargylic amines with aryl iodides, CO2 and CO under ambient pressure
Authors: Cai Shuai-Fang , Qiu Li-Qi , Huang Wen-Bin , Li Hong-Ru , He Liang-Nian
Published: April 2022

The palladium-catalyzed four-component carboxylative cyclization of propargylic amines, aryl iodides, CO2 and CO was developed, which has broad substrate scope and application prospects.
Electrostatic attraction induces cationic covalent-organic framework to pack inorganic acid ions for promoting proton conduction
Authors: Liu Xiao-Fei , Huang Sheng-Zheng , Lian Yu-Xiang , Dong Xi-Yan , Zang Shuang-Quan
Published: April 2022

The inorganic acid ions were encapsulated into a cationic COF via electrostatic attraction to enhance the proton conduction.
Templating effect of trans-2,5-dimethylpiperazine (TDMP) on the structural dimensionality of hybrid metal halides
Authors: Gautier Romain , Paris Michael , Massuyeau Florian
Published: April 2022

The templating effect of trans-2,5-dimethylpiperazine on the structures of 13 metal halide compounds has been investigated. The oxidation state and coordination sphere of the metal ions are the two key parameters related to the dimensionality of the inorganic network.
A microgrid-patterned silicon electrode as an electroactive lithium host
Authors: Ryou Myeong-Hwa , Kim Seung-Hyeok , Kim Sang-Woo , Lee Sang-Young
Published: April 2022

A microgrid-patterned silicon (MPS) electrode is presented as an electroactive Li host, which enables stepwise sequential Si lithiation/delithiation and Li plating/stripping reactions for a high-energy-density Li–metal full cell.
Light-induced beneficial ion accumulation for high-performance quasi-2D perovskite solar cells
Authors: Lian Xiaomei , Zuo Lijian , Chen Bowen , Li Biao , Wu Haotian , Shan Shiqi , Wu Gang , Yu Xuegong , Chen Qi , Chen Liwei , Yang Deren , Cahen David , Chen Hongzheng
Published: April 2022

2D PVSCs with “favorable” ion accumulation are realized via thermal–light post-treatment, which increases the built-in potential and device performance.
Metal–organic framework-derived MCF/PPy/MoS2 hybrid nanocomposites as an anode for lithium-ion batteries
Authors: Sun Peng , Chen Yiting , Wang Yongfei , Wang Yufeng , Zhang Zhiqiang
Published: April 2022

MCF/PPy/MoS2 delivers high reversible capacity and long cycling stability as a potential anode for lithium-ion batteries.
Controlled compositions of tellurium/vanadium co-doped into hydroxyapatite/ε-polycaprolactone for wound healing applications
Authors: Alsenany Nourah , Mansour S. F. , Eldera Samah S.
Published: April 2022

Hydroxyapatite (HAP) was co-doped with tellurium and vanadium ions via the co-precipitation method.
Synthesis of spiroindolenine-bearing chroman scaffolds based on a cyclization reaction of para-Quinone methides
Authors: Qin Hongmei , Xie Qimei , He Long
Published: April 2022

Diastereoselective synthesis of spirocyclic compounds containing chroman and indoline pharmacophores.
Systematic modifications of substitution patterns for property tuning of photoswitchable asymmetric azobenzenes
Authors: Küllmer Florian , Gregor Lucas , Arndt Hans-Dieter
Published: April 2022

Systematic investigations of substituted azobenzenes uncovered photoswitches that thermally relax in minutes, suitable for biological applications.
Regioselective C-3-alkylation of quinoxalin-2(1H)-ones via C–N bond cleavage of amine derived Katritzky salts enabled by continuous-flow photoredox catalysis
Authors: Kishor Gandhari , Ramesh Vankudoth , Rao Vadithya Ranga , Pabbaraja Srihari , Adiyala Praveen Reddy
Published: April 2022

An efficient, transition metal-free visible-light-driven continuous-flow C-3-alkylation of quinoxalin-2(1H)-ones has been demonstrated enabling excellent yields (72% to 91%) and shorter reaction time (0.81 min) as compared to batch system (16 h).
A mild and concise synthesis of aryloxy phosphoramidate prodrug of alcohols via transesterification reaction
Authors: Ying Hanglu , Yao Jie , Wu Fan , Zhao Yufen , Ni Feng
Published: April 2022

A synthesis of aryloxy phosphoramidate prodrug of alcohols enabled by a transesterification strategy is described here.
Standard-free single magnetic bead evaluation: a stable nanoplatform for prostate disease differentiation
Authors: Huang Zili , Xie Xiaobo , Xu Bei , Liu Rui , Hu Jianyu , Lv Yi
Published: April 2022

A standard-free stable single magnetic bead nanoplatform was proposed in this work. The use of metal signal ratio was directly applied for intrinsic biological fPSA to tPSA ratio evaluations for prostate disease differentiation.
Composition-driven archetype dynamics in polyoxovanadates
Authors: Kondinski Aleksandar , Rasmussen Maren , Mangelsen Sebastian , Pienack Nicole , Simjanoski Viktor , Näther Christian , Stares Daniel L. , Schalley Christoph A. , Bensch Wolfgang
Published: April 2022

Systems-based elucidation of the polyoxovanadate speciation reveals that heterogroup substitution can transform spherical kegginoids into tubular architectures in a programmable manner.
Access to tetracoordinate boron-doped polycyclic aromatic hydrocarbons with delayed fluorescence and aggregation-induced emission under mild conditions
Authors: Jiang Long , Wang Yu , Tan Dehui , Chen Xiaobin , Ma Tinghao , Zhang Baoliang , Yang Deng-Tao
Published: April 2022

A series of tetracoordinate boron-doped polycyclic aromatic hydrocarbons have been synthesized under mild conditions, featuring delayed fluorescence and aggregation-induced emission.
Intercalating methylene blue in molecular beacon for sensitive detection of salivary TNF-α towards early diagnosis of oral cancer
Authors: Zou Siyi , Wei Hui , Cui Xiaolin , Mak Wing Cheung , Li Xuejin , Liu Guozhen
Published: April 2022

An electrochemical molecular beacon-based biosensor is developed for the sensitive detection of TNF-α, which presents a non-invasive strategy for salivary cytokines detection towards the early diagnosis of oral cancer in a point-of-care fashion.
Controlling electron transport towards efficient all-solution-processed quantum dot light emitting diodes
Authors: Chen Hongting , Ding Ke , Fan Lianwei , Zhang Rui , Guo Runda , Zhang Jibin , Hou Lintao , Wang Lei
Published: April 2022

A high maximum external quantum efficiency of an all-solution-processed quantum dot light-emitting diode is demonstrated to be 28.6% by tailoring the electron injection for balancing the charge injection in a QLED.
A special issue of Essays in Biochemistry on current educational developments in molecular bioscience
Authors: Mello Luciane V. , Watson Helen R.
Published: April 2022

The 4th joint UK Biochemical Society and Federation of European Biochemical Societies (FEBS) education event, ‘Evolving Molecular Bioscience Education’ took place online on May 27 and 28, 2021. The event, continuing the biennial series, comprised the invited speakers’ talks, group discussions and other participants’ pre-recorded flash presentations. Although the UK dominated, there were also speakers and participants from other European countries and other continents. This special issue includes a varied collection of articles written by the speakers and other participants.
Predicting Treatment Outcomes in Rapid Syllable Transition Treatment: An Individual Participant Data Meta-Analysis
Authors: Ng Wei Lin , McCabe Patricia , Heard Rob , Park Veronica , Murray Elizabeth , Thomas Donna
Published: April 2022

Purpose:

The purpose of this study is to identify predictors of treatment outcomes in Rapid Syllable Transition Treatment (ReST) for childhood apraxia of speech through an individual participant data meta-analysis.

Method:

A systematic literature search identified nine ReST studies for inclusion. Individual participant data were obtained, and studies were coded for methodological design, baseline participant characteristics, service delivery factors, and treatment outcomes. Bivariate analyses were conducted to identify potential predictor variables. Multiple linear regressions were then performed to identify predictors of treatment outcomes.

Results:

Data for 36 participants from seven studies were included in the statistical analyses. In multivariate modeling, better performance on treated pseudowords posttreatment was predicted by higher baseline expressive language and Goldman-Fristoe Test of Articulation scores, lower speech inconsistency and percentage of vowels correct, and higher pretreatment accuracy on pseudoword targets. Better performance on untreated real words posttreatment was predicted by higher pretreatment accuracy on real words. Gains in performance and retention of gains were not significantly predicted by any individual variable or combination of variables.

Conclusions:

Baseline speech and expressive language skills and accuracy on pseudowords and real words were significant predictors of absolute posttreatment performance. Regardless of baseline characteristics, all children were statistically as likely to achieve gains during ReST and retain these gains for up to 4 weeks posttreatment. Large-scale prospective research is required to further examine the effects of dose frequency and co-occurring language impairments on treatment outcomes and the complex co-effects of percentage of vowels correct with other potential predictors.

Supplemental Material:

https://doi.org/10.23641/asha.19611714
Design of infinite‐dimensional distributed consensus filter for mobile sensor networks with packet losses
Authors: Xia Fuyang , Qian Xueming
Published: April 2022

In this paper, design of infinite dimensional distributed consensus filter is addressed for a class of spatially distributed processes utilising mobile sensor networks with multiple packet losses. The missing information is drawn into a sensor network in such a way that it conforms to a conditional probability distribution. A new framework for optimisation of mobile sensors is presented to enhance the performance of the filter. The goal of filtering issue is to construct a distributed consensus filter, for any possible measurements that are missing, such that the filtering error converge to bounded consensus in the mean square. Using the Lyapunov direct method and the spatial operator, certain sufficient criteria for the desired filter to satisfy the consensus performance criterion are developed. Lastly, a numerical simulation is performed to show that the suggested filter presented is effective.
Systematic errors on optical-SED stellar-mass estimates for galaxies across cosmic time and their impact on cosmology
Authors: Paulino-Afonso Ana , González-Gaitán Santiago , Galbany Lluís , Maria Mourão Ana , Angus Charlotte R. , Smith Mathew , Anderson Joseph P. , Lyman Joseph D. , Kuncarayakti Hanindyo , Rodrigues Myriam
Published: April 2022

Studying galaxies at different cosmic epochs entails several observational effects that need to be taken into account to compare populations across a large time-span in a consistent manner. We use a sample of 166 nearby galaxies that hosted type Ia supernovae (SNe Ia) and have been observed with the integral field spectrograph MUSE as part of the AMUSING survey. Here, we present a study of the systematic errors and bias on the host stellar mass with increasing redshift, which are generally overlooked in SNe Ia cosmological analyses. We simulate observations at different redshifts (0.1 <  z <  2.0) using four photometric bands (griz, similar to the Dark Energy Survey-SN program) to then estimate the host galaxy properties across cosmic time. We find that stellar masses are systematically underestimated as we move towards higher redshifts, due mostly to different rest-frame wavelength coverage, with differences reaching 0.3 dex at z ∼ 1. We used the newly derived corrections as a function of redshift to correct the stellar masses of a known sample of SN Ia hosts and derive cosmological parameters. We show that these corrections have a small impact on the derived cosmological parameters. The most affected is the value of the mass step ΔM, which is reduced by ∼0.004 (6% lower). The dark energy equation of state parameter w changes by Δw∼ 0.006 (0.6% higher) and the value of Ωm increases at most by 0.001 (∼0.3%), all within the derived uncertainties of the model. While the systematic error found in the estimate of the host stellar mass does not significantly affect the derived cosmological parameters, it is an important source of systematic error that needs to be corrected for as we enter a new era of precision cosmology.
Development of Digital Learning Application to Support Smallholder Dairy Farmers in Indonesia
Authors: Resti Yuni , Indriasari Sofiyanti , Widodo Bayu
Published: April 2022

The Indonesian government has facilitated farmers through the role of agricultural extensionists (AE). One of the agricultural businesses that have a high interaction with AE is dairy farmers. AE have the responsibility to connect regularly with dairy farmers. However, since the Covid-19 pandemic, AE have limitations in coordinating with farmers directly. Therefore, a strategy is needed to overcome so farmers can continue to receive counselling and AE can still carry out their duties. In addition, several routine training activities carried out to improve the ability of farmers in practical management have also been delayed. The study aimed to develop an online application in collaboration with the triple helix concept through the interaction of multiple sectors from government, education, and business. The study used the collaboration between farmers and cooperatives as partners who cooperate with government agencies through agricultural extensionists and knowledge institutions to share information related to livestock business development. The application was built using the Extreeme Programming (XP) methodology. The research was held by conducting independent interviews with relevant stakeholders such as dairy farmers, cooperative boards, AE, the Ministry of Agriculture, and academicians. The study facilitated dairy farmers and AE to interact online with specific features and discussions about dairy farming. Moreover, educators such as livestock teachers, lecturers, and trainers can also take advantage of this digital application, especially the updated information related to livestock farming practices.
Consumer Behavior on Beef Purchasing Decision in West Java
Authors: Dewi Intani , Aziz Husyairi Khoirul , Sahat Tua Manalu Doni
Published: April 2022

The aim of this study was to determine consumer behavior in making beef purchasing decisions. The study was carried out for 3 months from April to June 2021. Research data were obtained from 300 respondents who used to buy beef from several areas in West Java. Sampling was carried out by convenience sampling method and data collection techniques using questionnaires. The analytical method used was descriptive analysis. The results of the analysis concluded that some respondents bought meat to meet family needs (as side dishes), with an average consumption requirement of less than 1 kg per week. Purchases of beef have been planned and most of them buy meat from traders in traditional markets who have become customers. Information related to meat prices was obtained by respondents from relatives or close neighbors. Even so, the respondent has no influence from other people in making their meat purchasing decisions. The portion of beef that often purchased was tenderloin, and if the price of beef rises the respondent will buy chicken as a substitute. The beef usually purchased in the form of fresh meat with a custom cut based on the needs. Mornings and weekends are the most preferred time for consumers to buy beef. Most purchases were made in cash. Only a small part is done in non-cash (debit). Most of the respondents also already trust the existing regular meat traders and willing to recommend them to their relatives/others. The reason was that the meat sold fit to the price and quality.
Aquaculture and Its Impact of The Covid-19 Pandemic on The Fish Processing Industry: Case Study from Local Community
Authors: Kusumanti Ima , Oyinlola Muhammed A. , Nanda Muslikhah , Pangestu Putu Hamka Mugi
Published: April 2022

The Indonesian government has facilitated farmers through the role of agricultural extensionists (AE). Covid-19 pandemic has caused heavily impacts on the fisheries sector especially on the socio-economic conditions of the stakeholders, e.g., fishers, fish farmers, traders, as well as consumers. This impact on aquaculture is about its production is largely influenced by the demand from the food service sectors, processing factories, and export. Movement restrictions of fish farmers and less demand from consumers needs to maintaining the stocks of cultured commodities becomes more expensive as most of the products could not be harvested. From the snow ball sampling methods and research interview, the covid-19 pandemic makes the fish processing industry decrease more than 56%. It impacts 3 main activities, such as reducing demand for fish, low prices due to cancellation of shipment by buyer and lack of technical service provider. This decrease has a positive impact on the community to overcome the existing problem, diversifying the product and recognize need for new strategies to identify marketing opportunities that use technology.
A Development of Web Application for Hydroponic Monitoring Systems
Authors: Hermawan Hendi , Uddin Nur , Darajat Teddy Mohamad
Published: April 2022

A development of web application for a hydroponic monitoring system (HMS) is presented. The HMS is to collect data of hydroponic plants such as quality and quantity of nutrient solution. The HMS is integrated to internet such that the collected data is stored in a cloud server. The data can be accessed by users through a web application. This study is to develop the web application as an interface for accessing the HMS data in real time. The web development is done by implementing the user centred design (UCD) method. A study case of monitoring hydroponic temperatures is presented in this development. It resulted in a web application that presents real-time data of the temperatures in numerically as well as graphically. This web application provides an informative, attractive, and user-friendly interface of the HMS.
Performances of Debu and Kelabu Sentul Hens in the Different Rearing System at Poultry Breeding Development Center Jatiwangi Majalengka
Authors: Ihsani Hanifah Fauziyyah , Rukmiasih  , Fitriati Maya
Published: April 2022

The pattern of raising domestic chickens in Indonesia is still maintained traditionally, used as a side business and savings. Besides that, Domestic chickens still have high broodiness, slow growth, and reproduction rates, so changes are needed in the rearing system. Observations and data collection were carried out at Poultry Breeding Development Center Jatiwangi. Debu and Kelabu Sentul hens have reached the age of 29 weeks in the deep litter system, and colony cages were observed for three months by observing egg production, FCR, and Depletion. Debu Sentul hens reared in the deep litter systems had lower egg production, higher FCR, and Depletion (28.34%, 9.34, 21.05%, respectively) than those reared in colony cages (36.08%, 7.04, 4.76%, respectively). Kelabu Sentul hens reared in the deep litter systems had lower egg production, higher FCR, and Depletion (24.64%, 12.01, 15.51%, respectively) than those reared in colony cages (41.64%, 6.06, 10.86%, respectively). In conclusion, the performance of Kelabu and Debu Sentul hens reared in colony cages was better than in deep litter system cages. The performance of Kelabu Sentul hens reared in colony cages was better than Debu Sentul hens.
L’alphabet génétique élargi
Authors: Kaminski Pierre-Alexandre
Published: April 2022

Les génomes de bactériophages constituent la source la plus riche de nucléobases modifiées de toutes les formes de vie. Parmi celles-ci, la 2,6-diaminopurine (ou 2-aminoadénine), qui s’apparie avec la thymine en formant trois liaisons hydrogène, viole l’appariement des bases de Watson et Crick. La 2-aminoadénine, initialement trouvée dans le cyanophage S-2L, a également été détectée dans des bactériophages infectant des bactéries Gram-négatives et Gram-positives. La voie de biosynthèse de l’ADN contenant de la 2-aminoadénine ainsi que le mécanisme d’exclusion de l’adénine sont maintenant élucidés. Cet exemple de déviation naturelle d’un nucléotide de l’ADN ne représente qu’une des possibilités explorées par la nature et apporte une preuve de concept pour la biologie de synthèse d’acides nucléiques non canoniques.
Epigenetics for Drug Discovery: Dissecting the Effect of High
Antipsychotic Dosage and D2 Blockage on Peripheral DNA
Methylation
Authors: Adanty Christopher , Kim Julia , Strauss John , Qian Jessica , Remington Gary , Borlido Carol , Graff Ariel , Gerretsen Philip , De Luca Vincenzo
Published: April 2022

Introduction The relationship between genetic polymorphisms of
antipsychotic drug-metabolizing agents and drug receptors has been often
investigated. DNA methylation is a form of epigenetic modification that
regulates gene expression. Few studies have analyzed the relationship between
genome-wide methylation patterns and antipsychotic dosage. The primary aim of
this pilot study was to investigate the association between antipsychotic dosage
and genome-wide DNA methylation in patients with schizophrenia (SCZ).

Methods Current dosage of antipsychotic medications was assessed in 136
patients with SCZ. Dosage was standardized using three different methods:
chlorpromazine equivalent dose (CPZe), defined daily dose (DDD), and percentage
of Lexicomp maximum dose (PM%). DNA methylation was measured in white
blood cells. Antipsychotic dosage was the primary outcome variable in a model,
including genome-wide methylation status as the main predictor.

Results This study did not show any association between DNA methylation
and dosage variation for CPZe, PM%, and DDD. However, the probe
cg271403389 was consistently associated with antipsychotic dosage across the
three standardization methods. When looking at the genomic location of the most
significant probes, we found that 15% were intergenic, 23% were
in the distal promoter, 9% in the 3′untranslated region,
32% in the gene body, 3% in the 5′ untranslated region,
15% in the proximal promoter, and 3% in the first exon.

Discussion This study shows the importance of investigating the
relationship between DNA methylation and optimal antipsychotic dosage to
personalize treatment in SCZ. Future studies require larger prescription
databases to build on the results of this analysis.
One-Year Audiologic Monitoring of Individuals Exposed to the 1995 Oklahoma City Bombing
Authors: Van Campen Luann E. , Dennis J. Michael , Hanlin Renee C. R. , King Sandra B. , Velderman Amy M.
Published: April 2022

This longitudinal study evaluated subjective, behavioral, and objective auditory function in 83 explosion survivors. Subjects were evaluated quarterly for 1 year with conventional pure-tone and extended high-frequencies audiometry, otoscopic inspections, immittance and speech audiometry, and questionnaires. There was no obvious relationship between subject location and symptoms or test results. Tinnitus, distorted hearing, loudness sensitivity, and otalgia were common symptoms. On average, 76 percent of subjects had predominantly sensorineural hearing loss at one or more frequencies. Twenty-four percent of subjects required amplification. Extended high frequencies showed evidence of acoustic trauma even when conventional frequencies fell within the normal range. Males had significantly poorer responses than females across frequencies. Auditory status of the group was significantly compromised and unchanged at the end of 1–year postblast.

Abbreviations: ANOVA= analysis of variance, CF = conventional frequencies (0.25–8 kHz), EHF = extended high frequencies (10–20 kHz), LSD = least significant difference, psi = air pressure measured in pounds per square inch, dB pSPL = decibel re: peak sound pressure level, Q = quarterly evaluations, TNT = trinitrotoluene
Acoustic-Phonetic Approach toward Understanding Neural Processes and Speech Perception
Authors: Koch Dawn Burton , McGee Therese J. , Bradlow Ann R. , Kraus Nina
Published: April 2022

This review paper describes an “acoustic-phonetic” experimental approach aimed at understanding normal and abnormal speech perception processes from both a behavioral and an electrophysiologic perspective. First, we consider the relevant acoustic characteristics of speech and identify a set of acoustic-phonetic classes that represent the parameters most important for making an acoustic signal sound like speech. Second, we review what is known about the neurophysiologic representation of acoustic-phonetic speech parameters in animal and human subjects. Third, we describe how an acoustic-phonetic approach has been useful in understanding the biologic basis of some auditory learning problems in children and in characterizing the behavioral and neurophysiologic changes resulting from speech-sound training. Finally, we discuss these findings and how they may expand the diagnostic and rehabilitative repertoire of practicing audiologists.

Abbreviations: F0 = fundamental frequency; F1, F2, etc. = first formant, second formant, etc; fMRI = functional magnetic resonance imaging; JND = just noticeable difference; MMN = mismatch negativity
Habilitation and Management of Auditory Processing Disorders: Overview of Selected Procedures
Authors: Musiek Frank
Published: April 2022

This article describes three management approaches that can be used with children with auditory processing difficulties and learning disabilities. These approaches were selected because they can be applied in a variety of settings by a variety of professionals, as well as interested parents. The vocabulary building procedure is one that potentially can increase the ability to learn new words but also can provide training on contextual derivation of information, which is key to auditory closure processes. This procedure also helps increase language base, which can also enhance closure abilities. Auditory memory enhancement is a simple technique that involves many complex brain processes. This procedure reduces detailed information to a more gestalt representation and also integrates the motor and spatial processes of the brain. This, in turn, more fully uses working memory and helps in formulization and recall of important concepts of the sensory input. Finally, several informal auditory training techniques are discussed that can be readily employed in the school or home setting. These auditory training techniques are those that are most relevant to the kinds of deficits most often observed in our clinic.

Abbreviations: ΑΜΕ = auditory memory enhancement, AT = auditory training, CAPD = central auditory processing disorder, CV = consonant-vowel, CVC = consonant-vowel-consonant
Evaluation of energy storage systems for sustainable development of renewable energy systems—A comprehensive review
Authors: Gupta Ankush , Suhag Sathans
Published: April 2022

Energy storage systems (ESSs) have acquired enhanced importance with the extensive growth and development of renewable energy systems (RESs) to accomplish the increasing demand of power without causing adverse effects on environment. The ESSs help to eliminate the effects of intermittent nature of RESs by either injecting power into the RESs or extracting power from RESs depending on whether the RESs is in shortage or excess of power, respectively. In the present study, an elaborate review is presented, which gives the recent perspective of the ESSs technologies, their comparative analysis, and various specifications as well as evaluation through S-Strength, W-Window of opportunity, I-Intimidation, F-Failing, and T-Technical maturity analysis. Divergent ESSs, capable of power regulation, power quality maintenance, and enhancement of the grid reliability, have had huge significance in sustainable development of RESs, which is highlighted in this Review. In addition, this Review also brings out the recent research trends on ESSs, which include novel and significant innovations, achievements, and developments around ESSs.
Extension of on-chip thermometry of metal strips toward sub-10 K regime
Authors: Qiao Jiabin , Zhang Ding
Published: April 2022

One widely employed method to study thermoelectric effects in nano- and mesoscopic systems is by utilizing on-chip metal strips. However, the standard technique loses sensitivity in the sub-Kelvin temperature regime because the resistance of metal strips saturates. Here, we report a possible solution that can extend the working point from the conventional window (10 to 300 K) down to temperatures below 10 K. This method takes advantage of the unexpected resistance up-turn at T < 10 K in gold strips, presumably due to the Kondo effect. As a demonstration, we apply this technique to study a NbSe2 flake in the superconducting regime (<6 K).
Nanorod orientation control by swift heavy ion irradiation
Authors: Korkos Spyridon , Jantunen Ville , Arstila Kai , Sajavaara Timo , Leino Aleksi , Nordlund Kai , Djurabekova Flyura
Published: April 2022

Highly energetic ions have been previously used to modify the shape of metal nanoparticles embedded in an insulating matrix. In this work, we demonstrate that under suitable conditions, energetic ions can be used not only for shape modification but also for manipulation of nanorod orientation. This observation is made by imaging the same nanorod before and after swift heavy ion irradiation using a transmission electron microscope. Atomistic simulations reveal a complex mechanism of nanorod re-orientation by an incremental change in its shape from a rod to a spheroid and further back into a rod aligned with the beam.