bluuebunny/crossref_metadata_2025 huggingface dataset:
Knowledge, attitude, and practice of ultrasonics in endodontic treatment
Authors: Arun Nishitha , Ramesh Sindhu , Sankar Anjali
Published: May 2024

The use of ultrasonics (US) has greatly expanded in the field of dentistry. Over the past few decades, the application of US has increased substantially in endodontics owing to the predictable prognosis and ease of operation. The objective of this survey was to determine the knowledge, practice, and attitude of US in endodontics among the general practitioners, postgraduates, and endodontists. An electronic questionnaire containing 16 questions regarding the knowledge, attitude, and practice of US in endodontics was circulated among the general dentists, postgraduates, and endodontists and the responses were collected online. Data were statistically examined using IBM SPSS software. A total of 202 dentists (general dentists = 92, endodontists = 77, and postgraduates = 33) participated in the survey. Eighty-nine percent of them were aware of the use of US in endodontics, 36.1% of them preferred using US in the removal of pulp chamber calcifications, pulp stones, access refinement, and troughing hidden canals, and 61.4% chose <3% sodium hypochlorite for root canal irrigation with US. The cost of the ultrasonic unit and heat generation during procedures were considered the greatest limitation in using the US. The majority of the dentists were well aware of the use of US and its advantages in various endodontic procedures but they did not use it routinely in their practice. The use of US has been reported to have greatly increased the predictability of endodontic treatment.
Surface roughness changes of two composite resin restorative materials after thermocycling
Authors: Imtiaz Taanya , Ganesh S. Balaji , Jayalakshmi S.
Published: May 2024

Composite is usually a mixture of two phases. The composites used in dentistry have high durability. The individual properties of each phase are important to increase the mechanical property of the composite. This study aimed to determine the surface roughness changes of two composite resin restorative materials after thermocycling. Two of the commercially available composites were chosen (RestoFill and SwissTEC). The composite disks of dimension 10 mm diameter and 3 mm height were prepared using silicone molds, and every increment was light cured for 30 s. A stylus profilometer was used to assess the surface roughness prethermocycling, and then, the disks were subjected to an integrated thermocycler (T.S-4.4) for 1000 cycles. The postsurface roughness was obtained after the thermocycling process using the same stylus profilometer. The surface parameter values before and after thermocycling of the SwissTEC sample is less than that of RestoFill. There was a significant difference between the Rz and Rq values of the two different commercially available composite materials. Thus, the present study concludes that thermocycling influenced the surface roughness of composite resin and increased the surface roughness of both the RestoFill and SwissTEC composites.
The correlation of dentin elastic moduli and pH after exposed to combination of calcium hydroxide-propolis-propylene glycol
Authors: Widjiastuti Ira , Retnaningsih Fauziah Diajeng , Ramadhinta Yashinta , Widona Salsabila Nunki , Sukaton
Published: May 2024

Previous research states that adding propolis to powdered dental materials can increase the mechanical strength of the material. To analyze the differences and correlation of dentin elastic moduli and pH value after the exposure of calcium hydroxide, a mixture of propolis and calcium hydroxide, also a mixture of propylene glycol (PG), calcium hydroxide, and propolis. The dentine of bovine incisors was exposed into various compositions of a mixture of propolis, PG, and calcium hydroxide. The measurement of pH value and dentin elasstic moduli was performed after 7 days. To find difference among groups, one-way ANOVA was used, and Honestly significant difference (HSD) Tukey to compare each groups, followed by Pearson to define the correlation. A statistically meaningful difference was recorded between the groups (P < 0.05), and there was correlation between dentin elastic moduli and pH value. The more alkaline the environment, the more rigid the dentin.
Evaluation of flexural strength of bulk-fill composite resin after immersion in fruit juices
Authors: Janani K. , Ganesh S. Balaji , Jayalakshmi S.
Published: May 2024

Resin-based composites are increasingly employed in dental restorations due to their esthetics. Flexural strength is the characteristic feature of a substance, and it can be described as the stress present in the material just before it renders in a flexure test. The purpose of this study was to determine the flexural strength of a bulk-fill composite following immersion in fruit juices. Ten samples of bulk-fill type composite filling material were created, with four samples from Restofill (Group 1) and four samples from Ivoclar Vivadent (Group 2). Out of which, four specimens of each brand were included for immersion in fruit juices; two specimens were kept as control. The eight samples were put through a flexural strength test, with four remaining as controls, before being measured for maximum force and displacement on the Instron E3000 universal testing equipment. SPSS version 23 (IBM SPSS Statistics for Windows, Version 23.0. Armonk, NY: IBM Corp.) was used to examine the calculated flexural strength. The composite Ivoclar has a mean flexural strength of 20.52, whereas the composite Restofill has a mean flexural strength of 20.55. The P value for the independent sample t-test between the two groups, Ivoclar and Restofill, was 0.089 (>0.05), which is statistically insignificant. It is clear from this research that the bulk-fill composite resin Ivoclar has a low flexural strength when compared to the sample Restofill after immersion in fruit juices.
Effect of carbonated beverages on flexural strength of composite restorative material
Authors: Sushma B. , Ganesh S. Balaji , Jayalakshmi S.
Published: May 2024

Carbonated beverages have side effects on oral health and general health. Flexural strength is a prime mechanical property of restorative material. The aim of this study is to know the effect of carbonated beverages on the flexural strength of composite restorative material. Two types of composites (Ivoclar and Restofill) were used as samples for comparative evaluation of flexural strength. The sample size is 10 where 5 of each type of composites are included. The obtained samples were immersed in carbonated beverages. One sample of each type was used as a control. After 24 h, the flexural strength of all the 10 samples was evaluated using the formula (3FL/2bd2), and the values were tabulated. Average values and graphs were done using SPSS software. The results showed the changes in flexural strength of both Ivoclar and Restofill materials when immersed in carbonated beverages. The mean value of all the samples was taken, and the standard deviation was calculated. After immersion in carbonated liquids, the mean value of Ivoclar and Restofill samples is 58.9 and 35.01, respectively. P = 0.718 (>0.05), which was not statistically significant. After immersion in Coke, the Restofill group exhibits more flexural strength than the Ivoclar group composite; however, the strength reduces when immersed in Sprite as compared to the Ivoclar group composite.
Effect of carbonated beverages on flexural strength property of restorative glass ionomer cement
Authors: Ganesh S. , Ganesh S. Balaji , Jayalakshmi S.
Published: May 2024

Glass ionomer cement (GIC) releases fluorides and has good biocompatibility, carbonated drinks, sometimes known as fizzy drinks, are carbonated liquids that contain dissolved carbon dioxide, sweeteners, and natural or artificial flavoring. The aim of our study is to determine the flexural strength of GIC after immersing in carbonated beverages. Twelve samples of GIC filling material were used among which six samples prepared were from the Dtech brand and six were prepared from the Shofu brand for the in vitro study. Bar-shaped specimens were prepared from each group with the dimension of 2 mm × 2 mm × 25 mm. They were immersed in Sprite, fizz drinks, and in distilled water as a control group. The immersion period was 7 days. Then, the determination of maximum force and displacement was done using INSTRON E3000 (ElectroPuls) universal testing machine, then the collected data were used to determine flexural strength. The mean flexural strength of Dtech GIC was 24.84 ± 6.523 Mpa. The mean flexural strength of Dtech GIC was 18.57 ± 11.60 Mpa. The independent sample t-test showed that P = 0.247 (>0.05) which was statistically not significant. The flexural strength of GIC material decreased after being immersed in Sprite and fizz drinks.
Evaluation of tensile strength of surgical absorbable and nonabsorbable suture materials after immersion in different fruit juices
Authors: Anushya P. , Ganesh S. Balaji , Jayalakshmi S.
Published: May 2024

Surgical sutures are the most well-known surgical biomaterial device for ligating blood vessels. The primary goal of wound closure is to align wound margins to provide a closed and stable environment. Sutures with lesser tensile strength are susceptible to break throughout the healing process due to edema. To evaluate the tensile strength of absorbable and nonabsorbable suture materials after immersion in fruit juices. In this in vitro study, eight samples of commercially available sutures such as black silk and vicryl were divided into two groups: sample -1 were immersed in grape juice and sample -2 were immersed in lemon juice for 1 week. Universal testing machine INSTRON E300 UTM was used to test the tensile strength of various suture materials. The data are statistically analyzed using an independent t-test. The P < 0.05 was considered to be statistically significant. The mean of vicryl suture after immersion in grape and lemon juice was found to be 34.445 and 43.39; the mean value of black silk after immersion in grape and lemon juice was found to be 36.95 and 33.1. The tensile strength of black silk was slightly lower than the vicryl. Independent sample t-test showed that P = 0.561 (>0.05) which is statistically insignificant. Vicryl suture tested to have the highest tensile strength along with excellent knot holding capacity than black silk suture after immersion in fruit juices.
Evaluation of flexural strength of glass ionomer cement after immersion in fruit juices
Authors: Keerthana B. , Ganesh S. Balaji , Jayalakshmi S.
Published: May 2024

Glass ionomer cement (GIC) is a material used for restoration in dentistry. The stress in a material right before it yields in a flexure test is referred to as flexural strength. The aim of this study was to evaluate the flexural strength of two different glass ionomers after immersion in fruit juices. Five samples of D-Tech and Shofu GIC were prepared from each for the in vitro study. The eight samples were subjected to a flexural strength process and two were left as control which was followed by the determination of maximum force and displacement, then the collected data were used to determine flexural strength and analyzed using SPSS software (IBM SPSS Statistics for Windows, Version 22.0. Armonk, NY: IBM Corp). The mean value of flexural strength of the D-Tech GIC immersed in fruit juices is 4.4375 and the mean value of Shofu GIC immersed in fruit juices was 8.4975. Paired t-test was done between the two groups and the P value was 0.00, which is considered highly statistically significant. From this study, it is evident that the GIC brand Shofu has higher flexural strength compared to D-Tech. Flexural strength was decreased for both the GIC groups when immersed in grape juice.
Effect of brushing simulation on the surface roughness of soft-tissue liners
Authors: Kannan Sneha , Ganesh S. Balaji , Jayalakshmi S.
Published: May 2024

Soft denture liners evenly distribute functional loads over denture-bearing tissues. The liners aid in more evenly distributing the pressures of mastication to the underlying tissues by absorbing some of the masticatory forces. The study aimed to evaluate the brushing simulation influence on the surface roughness property of soft-tissue liners. A total of eight samples of Avue brand soft-tissue liners with the composition of varnish base and varnish catalyst were suspended into a standard template extracted and numbered sequentially and surface roughness was calculated using a stylus profilometer. A total of 30,000 cycles brushing were done, where the first group samples were brushed with Colgate toothpaste and the second group brushed with Dabur Red toothpaste using a toothbrush simulator (ZM3.8 SD Mechatronik). The data of both pre- and postbrushing values were recorded manually and statistically uploaded on SPSS software version 22 and values were represented in clustered bar graph forms. The significance value of Ra was 0.321. The significance value of Rq was 0.211. The significance value of Rz was 0.354, hence statistically, insignificant. In the present study, the surface roughness of soft-tissue liners is reduced to a minimal extent after brushing simulation.
Inhibition of bacillus spores germination by cinnamon bark, fingerroot, and moringa leaves extract
Authors: Kusuma Sri Agung Fitri , Septyadi Rendy , Sofian Ferry Ferdiansyah
Published: May 2024

This study was intended to discover the natural food preservatives by comparing the antibacterial effect of the ethanolic extract of cinnamon bark, finger root, and moringa leaves toward Bacillus cereus both the vegetative cells and spores. The antibacterial activities of the investigated extracts were assessed against cells using the agar diffusion method. Whereas the sporicidal test was performed by observing the colony growth, after various times of incubation (1, 3, and 5 h). The investigated extracts produced inhibition in a diameter ranging from 10.6 to 35.3 mm, and it can be classified that the extract of cinnamon bark was the most potent extract to inhibit the vegetative cells form, followed by fingerroot and the moringa leaves extract. Consistently, the ethanolic extract of cinnamon bark and fingerroot significantly yielded sporicidal activities higher than the moringa leaves extract. Both extracts exerted sporicidal activity within 1 h of contact time at the lowest test concentration of 5% w/v, whereas moringa leaves extract required a longer contact time (5 h) at higher concentration of 20% w/v. It can be concluded that cinnamon bark and fingerroot extract have great potential as effective food preservative candidates to inhibit the B. cereus growth than moringa leaves extract.
Need for practice management in undergraduate dental program - A cross sectional survey among the dentists
Authors: Aathira C. M. , Jeevanandan Ganesh , Govindaraju Lavanya
Published: May 2024

Advancement in technology, enhancement of knowledge, elevated expectations from the patients regarding the quality of treatment, and economic factors are emerging as challenges to dentists, which demand emerging dentists to have skills on patient management and leadership through an improved curriculum. The aim of the present study was to conduct a survey among dentists regarding the need for practice management in undergraduate dental program. A total of 100 dentists from Chennai were involved in the study. They were made to answer 15 questions which were circulated online. The obtained data were statistically analyzed using SPSS software. It is evident that the majority of the dentists emphasize on including practice management courses in the undergraduate dental program as it may be helpful for the budding dentists in their future private practices. It can be concluded that the opinion of the dentists is strong and beneficial and should be considered.
In silico identification of natural products from Centella asiatica as severe acute respiratory syndromecoronavirus 2 main protease inhibitor
Authors: Maya Putu Gita , Mahayasih Widyaswari , Harizal  , Herman  , Ahmad Islamudin
Published: May 2024

Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) main protease (S-CoV-2 Mpro) is one of the main targets in designing antiviral against SARS-CoV-2. Centella asiatica contains several triterpenoids, polyacetylenes, and benzoic ester derivative with various biological activities including anti-inflammation and antiviral. Triterpenoids from C. asiatica could act as inhibitors of S-CoV-2 Mpro. The main objective of this study was to identify potential natural products from C. asiatica as S-CoV-2 Mpro inhibitor with better pharmacokinetic through in silico molecular docking method. : As much as 11 compounds from C. asiatica were docked with S-CoV-2 Mpro (PDB ID: 6LU7) using AutoDock v4.2.6. Pharmacokinetic parameters of these compounds were assessed using SwissADME (free access webserver). Molecular docking results of 11 natural products indicated that asiatate 6 and asiatate 10 have strong interaction with quite similar binding free energy compared to native ligand ([FIGURE DASH]9.00 and[FIGURE DASH]9.58 kcal/mol compared to [FIGURE DASH]9.18 kcal/mol, respectively) with proper interaction to the catalytic dyad (His41 and Cys145). Pharmacokinetic analysis revealed that asiatate 4, asiatate 10, and asiatate 11 have poor pharmacokinetic properties. These results indicated that asiatate 6 could be recommended for further study as S-CoV-2 Mpro inhibitor.
Knowledge, awareness, and practice survey on conventional radiographic methods and processing among dental students
Authors: Begum Akifa , Jayaraman Mahalakshmi
Published: May 2024

X-rays are electromagnetic waves produced by an X-ray machine and are used to observe the internal structures of patients. The X-rays pass through the body before being detected by the detector file or a digital detector behind the patient. To analyze the knowledge, awareness, and practice on conventional radiographic method and processing among the dental students. A well-framed questionnaire consisting of 10 questions were prepared and distributed among 100 dental professionals and students through the online Google forms link. The data were collected, tabulated in Excel sheets, and analyzed using the SPSS software. The Chi-square test was used to assess the P value. Sixty-five percentage of the participants are aware of conventional radiographic methods and 35% are not aware. The Chi-square test was done giving a P = 0.001 (<0.05), which is statistically significant. The dental students as compared to the other specialties have adequate and efficient knowledge and are aware of the conventional radiographic methods and its processing.
Development and validation of an automated solid-phase extraction-LC-MS/MS method for the bioanalysis of fluoxetine in human plasma
Authors: Ahmad Ishtiaque , Ullah Zabih , Khan Mohd Ibrahim , Alahmari Abdullah Khaloofa , Khan Mohd. Faiyaz
Published: May 2024

A wide-range, specific, and precise liquid chromatography tandem mass spectrometric (LC-MS/MS)technique for quantifying fluoxetine (FLX) in human plasma was developed using the RapidTrace® automated solid-phase extraction (SPE) method; the analyte and internal standard (IS) were extricated on Oasis MCX SPE cartridges. Acetonitrile and 5 mM ammonium formate buffer (90:10 v/v) were used as mobile phase to achieve chromatographic separation on the reverse phase (C18 column). The analyte and IS were ionized using +ve electrospray ionization approach which was further traced by multiple-reaction monitoring on a tandem mass spectrometer. To quantify the FLX and FLX-d5, the parent-to-daughter ion transition of m/z of 310.0/44.1 and 315.0/44.0 was used, respectively. The method demonstrated a linear active limit of 0.20–30 ng/ml with recoveries ranging from 63.04% to 79.39% for quality control samples and 61.25% for IS samples. The concentrations over the calibration range demonstrated acceptable precision and accuracy. Due to the high inconsistency of the FLX concentration data, the minimum threshold of the assay was kept at 0.20 ng/ml. The flow rate was maintained at 500 μL/min, and the time for sample analysis for each injection was 3.5 min. The method was found to be specific, sensitive, and faster with minimum utilization of organic solvents and was utilized further for metabolic and pharmacokinetic studies.
Awareness on the management strategy of stitch abscess among dental students
Authors: Bharathi R. , Ganapathy Dhanraj , Ahmed Nabeel , Maiti Subhabrata , Pandurangan Kiran Kumar
Published: May 2024

Stitch abscesses are abscesses that develop following surgical procedures as a result of suture infections. Because of nonabsorbable sutures, the material reacts with connective tissue, generating adhesions around the stitch. The use of this type of material increases the risk of infection. The aim of this study was to establish awareness on the management strategy of suture stitch abscess among dental students. An online survey consisting of ten questions about suture stitch abscess along with sociodemographic factors was circulated among equal numbers of 2nd years, 3rd years, final years, and compulsory rotatory residential internship (CRRI). The data obtained from the questionnaire were transferred to Microsoft Excel and imported to SPSS software for statistical analysis. Interns are more aware about the complications and management strategy of suture stitch abscesses compared to others. Awareness on the management strategy of suture stitch abscess is important, and it helps to reduce the side effects to improve the outcomes. Educating the risk factors, signs and symptoms, and management strategy is the best way to help raise awareness.
A systematic review
Authors: Hidayat Syahrul , Ibrahim Faisal Maulana , Suhandi Cecep , Muchtaridi Muchtaridi
Published: May 2024

Non-small cell lung carcinoma (NSCLC) is a type of lung cancer with the highest prevalence and mortality rate worldwide. Many cases of this type of cancer are overexpression on epidermal growth factor receptor (EGFR). The use of currently available EGFR inhibitors as one of the treatment options for NSCLC still shows various shortcomings, especially the high failure rate of therapy due to resistance. It is important to find NSCLC drug candidates with EGFR inhibitory activity. There are various published articles and it is prominent to draw evidence-based scientific conclusions as a basis of decision-making to select potential compounds for further research. Polymer matrix composites and ScienceDirect are used as a database for article screening. Research using molecular docking method targeted to EGFR with parameters of Gibbs energy and amino acid interactions between ligands and drug targets are included in inclusion criteria. Compounds that achieve docking parameters and have comparable activity to NSCLC guideline drugs are conscientiously ranked. There are only 11 compounds that achieved the docking parameters and had comparable EGFR inhibitory potential. Top-rated compounds include 1,3,5-trisubstituted pyrazoline (3c), 1,3,5-trisubstituted pyrazoline (6c), 1,3,5-trisubstituted pyrazoline (8d), N-(3,4-Dimethylphenyl)-2-[(4-oxo-3-(4-sulfamoylphenyl)-3,4-dihydrobenzo[g] quinazolin-2-yl) thio] acetamide. The top-rated compounds can be used and considered for further research processes.
Mode action prediction of catechin from Uncaria gambir Roxb. against UDP-N-acetylenolpyruvyl-glucosamine reductase (MurB enzyme) of Streptococcus mutans
Authors: Dharsono Hendra Dian Adhita , Wibisono Lydiawati , Hayati Ayu Trisna , Apriyanti Eti , Satari Mieke Hemiawati , Kurnia Dikdik
Published: May 2024

The prevalence of oral health problems in the global population is still high, especially dental caries, which is considered a multifactorial disease involving the role of bacteria, namely Streptococcus mutans. Gram-positive bacteria metabolize carbohydrates and sugars and convert them into lactic acid, causing dental caries. The peptidoglycan (PG) layer at the outer surface of the bacteria acts as protection. MurB enzyme is known for its contribution to PG biosynthesis. Gambir (Uncaria gambir Roxb.) is famous for many efficacies. Previous studies show that catechin from herb plants such as U. gambir has antibacterial activity. This study aimed to evaluate and predict the antibacterial activity of catechin from U. gambir against the MurB enzyme, which contributes to forming the bacteria PG, with an in silico approach. The structure of the MurB enzyme was collected from UniProt, and the ligands (catechin and chlorhexidine) structures were obtained from PubChem. The AutoDock software was used to dock both ligand and MurB enzyme visualized using PyMOL and analyzed using BIOVIA. The results showed that catechin has a binding affinity of more than − 7 kcal/mol against the MurB enzyme, and chlorhexidine has a higher binding affinity than catechin. Both catechin and chlorhexidine have similar amino acids attachment by hydrogen bonds. The results showed that catechin has competitive antibacterial activity against chlorhexidine in inhibiting the MurB enzyme.
Cytotoxicity and phytochemical profiles of Phyllanthus emblica stem barks with in silico drug-likeliness
Authors: Quranayati Quranayati , Iqhrammullah Muhammad , Saidi Nurdin , Nurliana Nurliana , Idroes Rinaldi , Nasution Rosnani
Published: May 2024

Out of numerous reported medicinal plants, Phyllanthus emblica has been reported to possess a strong antidiabetic potential and other pharmacological effects. This research aimed to identify the phytoconstituents in the extracts of P. emblica stem barks and hypothesize their antidiabetic potentials based on in silico drug-likeliness. Simplicia of P. emblica powder was sequentially macerated at room temperature (24 h) using n-hexane, ethyl acetate, and methanol solvents. Phytochemical profiles of the extract were investigated qualitatively using reagents, followed by gas chromatography–mass spectrometry (GC-MS) analysis. All phytocompounds were then analyzed for their pharmacological properties and predicted bioactivities on molinspiration. Cytotoxicity of each extract was evaluated using the brine shrimp lethality test. As many as 18 compounds (from GC-MS), were identified in all extract samples from P. emblica stem barks. Based on in silico drug-likeliness, methanol extract contained the most potentially bioactive compounds (16α-hydroxycleroda-3,13 (14) Z-dien-15,16-olide; 14-. beta.-H-pregna; and isochiapin B). Isochiapin B was revealed as the only compound that had no violation of the rule of five. All three compounds could hypothetically contribute to the antidiabetic activity of the methanol extract from P. emblica stem barks by inhibiting diabetes-related enzymes and interacting with nuclear receptors. Moderate cytotoxicity of ethyl acetate and methanol extract, respectively, further suggests their bioactivities.
Knowledge, attitude, and practice survey on the use of stamp technique for the management of Class I caries in molars among undergraduate students in dental schools
Authors: Reshawn M. Infant , Kolli Sankeerthana
Published: May 2024

Stamp technique is a recently developed technique that involves of creating an index which records the occlusal anatomy of posterior teeth before cavity preparation. This technique is beneficial in cases where the caries is involving the occlusal surface with little or no damage to the occlusal anatomy or the marginal ridges. The aim of the study is to assess the knowledge and practice on the use of stamp technique for the management of Class 1 caries in molars among undergraduate students. A questionnaire-based study was conducted among 100 dental students in Saveetha Dental College. The questionnaire contained 17 questions that were circulated through Google Forms and received dichotomous responses. The data were collected as an ordinal variable and the collected data were analyzed using SPSS software, and Chi-square test and descriptive analysis were done. This study shows that almost 37% of the respondents were interns and final-year students, 62% of them felt carving would be easier to attain the proper occlusal morphology, 55% of them prefer both flowable and packable composites, and 63% felt stamp technique would be technique sensitive. There was a significant association between the year of study and difficulties faced in occlusal stamp technique (P = 0.014 < 0.05). Undergraduate students were moderately aware of the stamp technique in composite restorations. More detailed knowledge on composite finishing and polishing should be emphasized in the undergraduate curriculum.
Extraction, characterization, and anticancer potential of extracellular polymeric substances from marine actinobacteria of Streptomyces species
Authors: Noufal Z. Mohamed , Sivaperumal Pitchiah , Elumalai P.
Published: May 2024

To check the ability of the anticancer activity, the extracted extracellular polymeric substances (EPS) from marine actinobacteria were done. Potential of the anticancer activity of EPS which was extracted from marine actinobacteria of Streptomyces species through an assay called MTT. Marine actinobacterial isolation, identification and micromorphology of the strain, and biochemical analysis were performed (Shirling and Gottlieb, 1966). The production of EPS from marine actinobacteria was quantified(P. Sivaperumal et al., 2018). Carbohydrate content in the EPS was quantified, and MCF-7 cell proliferation was done using an MTT assay. EPS-producing marine Streptomyces was isolated and identified. The production of EPS and their protein, carbohydrate, lipid, and other parameters were estimated. Further, the EPS showed more than 50% of inhibition after 72 h using the MTT assay in the MCF-7 cancer cell line. The present study exhibited that EPS from marine Streptomyces species has significant anticancer activity.
Antibacterial potential of inulinase enzyme obtained from Nocardiopsis sp.
Authors: Neha N , Sivaperumal Pitchiah , Roy Anitha
Published: May 2024

The enriched nutritional and functional properties of inulinase with wide attention are considered commercial/industrial food enzymes. It can be produced by many microorganisms such as yeasts, fungi, and bacteria. Nocardiopsis is a genus under Actinomycetes, which has biotechnologically important microorganisms. This study aims to isolate and identify marine Actinomycetes Nocardiopsis species and to evaluate the antibacterial potential of the inulinase enzyme obtained from it. Marine actinobacteria (Nocardiopsis sp.) were isolated from sediment samples on YM agar. The isolate was identified by biochemical analysis of cell walls (amino acid and sugar). Enzyme screening assay was performed with temperature and pH influence in the production inulinase enzyme production. Antibacterial activity and minimal inhibitory activity of inulinase enzyme were performed with Staphylococcus, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Antimicrobial testing revealed that with higher concentrations of inulinase enzyme, the zone of inhibition of bacterial growth increased, and the minimum inhibitory concentration of inulinase enzyme that prevented the growth of bacteria was close to the standard tetracycline. Inulinase enzyme obtained from Nocardiopsis species shows good antibacterial activity against Staphylococcus aureus, K. pneumoniae, and P. aeruginosa in comparison to the standard, tetracycline.
Knowledge and awareness on Balo's disease among dental students
Authors: Ananya Bathala , Veeraraghavan Vishnupriya , Kavitha S. , Selvaraj J. , Gayathri R.
Published: May 2024

Balo's disease is an uncommon central nervous system disorder causing demyelination and is a form of multiple sclerosis. Concentric sclerosis, leukoencephalitis periaxialis concentrica, is also known as Balo's disease. Concentric sclerosis signalizes the bands of intact myelin and alternating rings of myelin loss present in several parts of the brainstem and brain. The present study aimed to assess and create awareness about Balo's disease among dental undergraduates. The study included an online survey about the knowledge and awareness of Balo's disease among dental students. A questionnaire was prepared, uploaded, and distributed in an online portal. The latest software version of SPSS was used to analyze the collected data. Descriptive statistical analysis and Chi-square test were done, and the P value was calculated. The survey showed that 54.55% of males and 45.45% of females participated in this survey. Most of the respondents (87.88%) knew about Balo's disease, whereas only 12.12% of participants were not aware of the disease. It was found that most of dental students are aware of Balo's disease. The study also showed that male respondents are more aware than the females.
Melanin pigments from sediment-associated Nocardiopsis sp. marine actinobacterium and antibacterial potential
Authors: Sundar R. , Sivaperumal Pitchiah
Published: May 2024

To extract the melanin pigment from marine microbes and their biological potential, the present study was done. Isolation and identification of the melanin-producing Nocardiopsis sp. were obtained from the sediment samples. Zone of inhibition and minimal inhibitory concentration test was performed using melanin. Melanin was extracted from sediment-associated marine Nocardiopsis sp. In the present study, marine actinobacterium was identified by the conventional method, and the isolate was identified as Nocardiopsis sp. Melanin was extracted, and antibacterial activities were performed against different pathogens and the highest zone of inhibition is more in the E. coli while related to another two species. From previous observation done by Fu et al., they have said that marine actinobacteria have the ability of antimicrobial activity, which is very much helpful in producing the potential antimicrobial drugs this was similar to our study that marine actinobacteria have the capability to produce melanin pigment, and at the same time, it helps as to show the antibacterial activity. We concluded that melanin is produced by the Nocardiopsis sp. We also found that melanin extracted from the Nocardiopsis sp. of marine actinobacterium also has an antibacterial effect.
Innovated formulation of oseltamivir powder for suspension with stability study after reconstitution using a developed ion-pair reversed phase high-performance liquid chromatography method
Authors: Hasson Kahtan Jassim
Published: May 2024

Oseltamivir is an antiviral neuraminidase inhibitor used for the treatment and prophylaxis of influenza infection with viruses A and B. The mechanism of oseltamivir antiviral activity is by inhibiting the activity of the viral neuraminidase enzyme present on the surface of the virus, which stops viral replication and infectivity. Oral suspensions of oseltamivir phosphate are dispensed orally capsules and suspension. However, the use of oral suspension for pediatric administration is preferable and is prepared as a powder for suspension. The reconstituted suspension degrades rapidly within a few days. The objective of this work is to establish a stable formulation of oseltamivir phosphate as a suspension and to assure the stability conditions for prolonged use after reconstitution in aqueous form. In addition, this required formulation should maintain a high rate of dissolution, which subsequently leads to higher bioavailability. In this study, oseltamivir forms an inclusion complex with the natural and safe polymer hydroxypropyl beta-cyclodextrin, which resembles a host because its structural cavity carries the oseltamivir molecule in the aqueous preparation and provides a protective property against environmental challengers. In addition, a high-performance liquid chromatography (HPLC) stability-indicating method of analysis has been developed using an ion-pair reversed-phase HPLC technique that is validated for precision, accuracy, reproducibility, and specificity for the determination of oseltamivir in suspension. The results of this work show the relatively long shelf life of the reconstituted oseltamivir oral powder for suspension in the new pediatric formulation, and the developed HPLC method was precisely suitable for stability study.
Awareness on current status of usage of ritonavir in the management of covid among dental students
Authors: Ramamoorthy Jagadheeswari , Ganapathy Dhanraj , Pandurangan Kiran Kumar , Ahmed Nabeel , Maiti Subhabrata
Published: May 2024

Ritonavir's pharmacodynamics raise questions regarding whether medication concentrations high enough to block the SARS-CoV-2 proteases can be obtained. Ritonavir may have a therapeutic benefit at any stage of the disease, although pharmacodynamic issues and the absence of clinical benefit data among hospitalized COVID-19 patients cast doubt on this claim. To study the awareness on current status of usage of ritonavir in the management of COVID among dental students. This study, which is survey-based, involved a sample of 100 individuals and was carried out on the website Survey Planet. The study included undergraduate and graduate dental students. The dental students were given a questionnaire containing 10 questions. Their responses were gathered. The number and percentage were used to summarize the data. Dental students from Chennai showed adequate knowledge of COVID-19 in the medical environment. Postgraduate students were more aware about the current status of usage of Ritonavir for COVID-19. This study demonstrates the urgent necessity for all health-care workers to regularly implement educational interventions and training program on infection control procedures for COVID-19.
Formulation and characterization of orodispersible tablet of glimepiride
Authors: Kinani Ahmad AB Yosef , Taghi Hassanien Sagban
Published: May 2024

The present study is regarding, Glimepiride is one derivatives of sulfonyl urea used in the treatment of Type II DM which classified as class-II (BCS) of high permeability and low degree of solubility. The endeavor is to improve its solubility by solvent vaporization method to enhance the rate of dissolution of glimepiride. Soluplus (Polyvinyl caprolactampolyvinyl acetate-polyethylene glycol graft co-polymer), PVP k40 (Polyvinylpyrrolidone) and PEG k5 are blended with the drug in various proportions (1:1,1:3) and prepared Soluplus1, Soluplus2, PEG1, PEG2, PVP1 and PVP2 as solid dispersion. The optimized formula of solid dispersion PVP1 is added to sodium starch glycolate and cross-carmellose. The disintegration profile will appear diminished in the drug release from the dosage form at a determined period of time. Differential scanning calorimetry appeared to a reduction in its crystallinity in solid dispersions. Scanning electron microscope and particle size analysis show a reduction in the drug particle size as solid dispersions. Fourier transform infrared spectroscopy does not show an interaction between them. Hence, that PVP1 batch will be considered from nine oral dissolving tablets dosage form. Finally, orally disintegrating tablets are estimated for various parameters; for instance, disintegration time, the content of the drug, wetting time, and in vitro release profile show a conventional result. The selected formula F6 shows a good result in disintegration time during 13-second and in-vitro drug release profile achieves 96% at the end of 40 minutes.
Comparison between conventional and micro-assisted periodontal surgery
Authors: Katariya Chanchal , Rajasekar Arvina
Published: May 2024

Microsurgery is a minimally invasive procedure that uses a surgical microscope, specially designed equipment, and suture materials. Even though this equipment and expertise of numerous surgeries are required to meet patient esthetic reckoning, doctors must be ready to invest time and effort into becoming familiar with novel surgical methods and devices. The ambition of this case series is to compare conventional macro surgery and microsurgery in terms of clinical approach. This study included four cases, two flap surgery, and two root coverage. Clinical parameters for root coverage, increase in keratinized tissue (KT), gain in clinical attachment level (CAL) and complete root coverage (CRC), dentin hypersensitivity index-Schiff's index and for flap surgery, probing depth, clinical attachment level. Healing and pain analysis were done. There was no significant difference seen between conventional and clinical outcomes of a microsurgical technique such as clinical attachment level, probing depth, increase in KT, gain in clinical attachment level (CAG), and CRC, dentin hypersensitivity index-Schiff's index. When patient-based outcomes such as healing index and Visual Analog Scale, a significant difference was seen. If a microsurgical method is used instead of a traditional macroscopic approach, the early healing index can be significantly improved and there will be less postoperative pain.
Awareness on D-dimer assay among dental students
Authors: Anjum Shamaa , Ahmed Nabeel , Ganapathy Dhanraj M. , Maiti Subhabrata , Pandurangan Kiran Kumar
Published: May 2024

D-dimer molecules are formed by the degradation of cross-linked fibrin during the process of fibrinolysis. The formation of D-dimer requires the activity of activated factor XIII (factor XIIIa), plasmin, and thrombin. To assess the awareness about D-dimer assay among dental students. A cross-sectional study was done among 100 dental practitioners through an online survey. The survey consisted of 10 semiclosed prevalidated and reliable questionnaires based on the knowledge, attitude, and practice of the dentists on D-dimer assay. Descriptive and inferential statistics were performed to report the responses of the participants. Most participants did not know what a D-dimer assay is (55%). Forty-six percent of participants responded that the D-dimer assay is used to rule out serious blood clots. Fifty-four percent of the participants responded that fibrin D-dimers are formed when fibrin strands are formed. The current study shows that the knowledge about D-dimer assay is more in CRRI than in participants of junior year of study. Thus, more rigorous educational programs should be initiated to further enrich the knowledge among dental students.
Awareness of usage of Vicryl suture material in oral surgical procedures
Authors: Sagana M. , Ahmed Nabeel , Ganapathy Dhanraj , Maiti Subhabrata , Pandurangan Kiran Kumar
Published: May 2024

Oral surgical procedures constitute a very important part in the broad scope of general dentistry. This necessitates a significant need for dental students to know about the suture materials. This study aims to assess the awareness of Vicryl suture material in dental surgical procedures. The descriptive survey was attended among 237 dental students. Results were analyzed using Microsoft Excel and using SPSS software version 23.0. The majority (96%) of the dental students were aware of Vicryl suture material. Approximately 75% and 66% knew that Vicryl suture was absorbable and braided, respectively. However, only 33% knew that Vicryl suture would get fall off in 2 weeks. According to the statistics, there is no significant difference between gender and awareness of Vicryl suture material (Chi-square; P = 0.14, not significant). Dental students have adequate knowledge about Vicryl suture material.
Awareness about Patterson syndrome among dental students
Authors: Dhakshinya M. , Veeraraghavan Vishnu Priya , Gayathri R. , Kavitha S.
Published: May 2024

The aim is to create awareness about Patterson syndrome among dental students. Patterson-Stevenson-Fontaine syndrome is a very rare condition marked by irregular facial bone and tissue growth (mandibulofacial dysostosis) as well as limb abnormalities. A recessed jaw (retrognathism), cleft palate, and external ear defects are all possible symptoms of this disorder. A total of 112 undergraduate dental students participated in a longitudinal cross-sectional sample. To assess college students' awareness about Patterson syndrome, a self-administered, closed-ended questionnaire was developed and distributed. The only language allowed was English. The results were analyzed in SPSS software version 23. 10.71% of females and 14.29% of males were aware about Patterson syndrome. 32.14% of females and 38.39% of males were aware that Patterson syndrome was a rare adrenal disorder. We can conclude that very few of the population which was only 25% of the dental students were aware about Patterson syndrome and this survey helped in creating awareness about this syndrome.
Dentist perspective on knowledge and practice toward Ellis class 3 tooth fracture
Authors: Imtiaz Taanya , Adimulapu Hima Sandeep , Pandiyan Indumathy , Bhandari Sarita
Published: May 2024

Traumatic dental injuries otherwise referred to as dental trauma is common in dental practice. The etiology of dental trauma is mostly falls and accidents in case of children and young adults. The treatment provided should be quick and effective which depends on the extent of the dental trauma, the injury might expose enamel, dentine and pulp of the affected teeth. Ellis class 3 involves all the 3 layers mentioned, so there should be quick measures taken. A survey was conducted among dental practitioners to assess their knowledge and practice experience about Ellis class 2 fracture. Detailed questionnaire was created with questions about Ellis class 3 tooth fracture and was circulated to 100 dental practitioners in Chennai, Tamilnadu through google forms. The collected data was analyzed using SPSS software. The knowledge and practice of Ellis class 3 trauma was 85%, the dental practitioners have adequate knowledge about the various clinical, diagnostic aids used and are aware to provide the correct treatment for each case. Based on the responses from the dental practitioners there is not much practice of Ellis class 3 dental trauma in their dental practice. Thus the present study concludes that the knowledge and practice of Ellis class 3 teeth fracture is good among dental practitioners and the various aspects are well known by dentists.
Awareness about the significance of acid–base balance of saliva in maintaining oral health
Authors: Suresh Charanya , Veeraraghavan Vishnupriya , Jayaraman Selvaraj , Gayathri R. , Kavitha S.
Published: May 2024

Saliva is a multilayered secretion. The quality of saliva is just as crucial as the quantity. Saliva is necessary for preserving the integrity of oral structures, personal connections, digestion, and acid–base balance. The acid–base balance in one's oral environment is always being challenged. It happens every time we eat a meal, and when microorganisms are present. At a pH of 5 or 5.5, the demineralization of the enamel and hard tooth structures occurs. This study aimed to raise awareness about the importance of acid–base balance of saliva in dental health. A self-structured questionnaire was prepared to assess the awareness on the importance of acid–base balance in maintaining oral health. The questionnaire consisted of 20 questions, and the results were plotted in a graph. The Chi-square test was employed for evaluating the data, which was done with SPSS software. The study indicated that 91.74% of the participants were aware of the salivary pH in case of healthy gingiva, 34.82% of the respondents were aware of the salivary pH in case of chronic gingivitis, and 20.33% of the respondents were aware of the salivary pH in case of chronic periodontitis. We also correlated our results with gender and year of study. Oral health plays a major role in identifying systemic diseases and hence enough knowledge about various aspects about the oral cavity and its manifestations needs to be created among dental students to ensure that they become efficient dental surgeons in the future. Even though a fair amount of people knew the pH of clinically healthy gingiva, most of them did not know the pH at which periodontitis or gingivitis was caused. Hence, creating awareness about this topic stands vital. Further studies are required for a better understanding of salivary components and how they affect the caries process. The utilization of modified oral molecules as medicinal agents could become a reality with this understanding. Our findings imply that being aware of dietary composition has an impact on acid–base balance. To maintain the correct acid–base balance, more awareness of protein consumption is required.