bluuebunny/crossref_metadata_2025 huggingface dataset:
Redescription of two Australian cladocerans of the subfamily Aloninae Dybowski & Grochowski (Cladocera: Anomopoda: Chydoridae)
Authors: SINEV ARTEM Y.
Published: March 2022

Morphology of two Australian water fleas, Alona rigidicaudis (Smirnov, 1971) and Coronatella (Coronatella) novaezealandiae (Sars, 1904) (Crustacea: Cladocera) was studied by optical and scanning electron microscopy. Morphology of the thoracic limbs was studied in detail. Previously unknown ephippial females and males are described for both species. Alona rigidicaudis is close in morphology to the simplex-group of the genus Anthalona Van Damme, Sinev & Dumont, 2011, but lack the main distinctive feature of Anthalona, the presence of cosmaria of lateral head pores. Morphology of Coronatella (Coronatella) novaezealandiae (Sars, 1904) is typical of the genus. Affinities and taxonomic position of studied species are discussed.
Heteragrion calafatiensis (Odonata: Heteragrionidae) sp. nov. from Northern Brazil
Authors: MENDOZA-PENAGOS CRISTIAN CAMILO , JUEN LEANDRO , VILELA DIOGO SILVA
Published: March 2022

The Neotropical genus Heteragrion Selys, 1862 is the second most speciose among the Neotropical Zygoptera. However, most of its species are poorly known regarding their biology, distribution, and taxonomy, especially those from undersampled areas like Northern Brazil. Here we describe Heteragrion calafatiensis sp. nov. from the coastal environments of the Brazilian Amazon of Pará state (Holotype ♂, Brazil, Pará, municipality of Magalhães Barata, near the Marapanim River, (-0.863291, -47.668058, 20 m, 26.ix.2021), C.C. Mendoza-Penagos leg, in UFPA). This is the nineth Heteragrion species recorded for the Northern region of Brazil. The new species can be separated from congeners by cercal morphology and thoracic coloration pattern.
A new species of the genus Tonoscolex Gates, 1933 (Clitellata: Megascolecidae) from India
Authors: AHMED SHAKOOR , EMILIYAMMA K. G. , MARIMUTHU NITHYANANDAM , SAJAN SHEIKH , JULKA J. M.
Published: April 2022

Tonoscolex kalimpongensis Ahmed & Julka sp. nov. is described from Neora Valley National Park in the Kalimpong district of West Bengal, India. The new species is easily distinguished by the presence of one pair of spermathecal pores at intersegmental furrow 7/8. An updated checklist of the genus Tonoscolex and an identification key to the Indian Tonoscolex species are provided as well.
Comparison of Lagrangian Superdroplet and Eulerian Double-Moment Spectral Microphysics Schemes in Large-Eddy Simulations of an Isolated Cumulus Congestus Cloud
Authors: Chandrakar Kamal Kant , Morrison Hugh , Grabowski Wojciech W. , Bryan George H.
Published: April 2022

Advanced microphysics schemes (such as Eulerian bin and Lagrangian superdroplet) are becoming standard tools for cloud physics research and parameterization development. This study compares a double-moment bin scheme and a Lagrangian superdroplet scheme via large-eddy simulations of nonprecipitating and precipitating cumulus congestus clouds. Cloud water mixing ratio in the bin simulations is reduced compared to the Lagrangian simulations in the upper part of the cloud, likely from numerical diffusion, which is absent in the Lagrangian approach. Greater diffusion in the bin simulations is compensated by more secondary droplet activation (activation above cloud base), leading to similar or somewhat higher droplet number concentrations and smaller mean droplet radius than the Lagrangian simulations for the nonprecipitating case. The bin scheme also produces a significantly larger standard deviation of droplet radius than the superdroplet method, likely due to diffusion associated with the vertical advection of bin variables. However, the spectral width in the bin simulations is insensitive to the grid spacing between 50 and 100 m, suggesting other mechanisms may be compensating for diffusion as the grid spacing is modified. For the precipitating case, larger spectral width in the bin simulations initiates rain earlier and enhances rain development in a positive feedback loop. However, with time, rain formation in the superdroplet simulations catches up to the bin simulations. Offline calculations using the same drop size distributions in both schemes show that the different numerical methods for treating collision–coalescence also contribute to differences in rain formation. The stochastic collision–coalescence in the superdroplet method introduces more variability in drop growth for a given rain mixing ratio.
Global Middle-Atmosphere Response to Winter Stratospheric Variability in SABER and MLS Mean Temperature
Authors: Smith A. K. , Pedatella N. M. , Bardeen C. G.
Published: April 2022

Satellite observations of middle-atmosphere temperature are used to investigate the short-term global response to planetary wave activity in the winter stratosphere. The focus is on the relation between variations in the winter and summer hemispheres. The analysis uses observations from Thermosphere–Ionosphere–Mesosphere Energetics and Dynamics (TIMED) Sounding of the Atmosphere using Broadband Emission Radiometry (SABER) for 2002–21 and Aura Microwave Limb Sounder (MLS) for 2004–21, and reanalysis temperatures and winds from MERRA-2 for 2002–21. We calculate temporal correlations of the Eliassen–Palm flux divergence in the winter stratosphere with global temperature. Results show a robust perturbation extending to midlatitudes of the Southern Hemisphere (SH) stratosphere during Northern Hemisphere (NH) winter. An increase in wave forcing is followed by a decrease in temperatures over the depth of the stratosphere in the SH, peaking at a lag of 3 days. Summer mesospheric temperature perturbations of the opposite sign are seen in many winters. Comparable signals in the NH summer middle-atmosphere are present during some SH winters but are weaker and less consistent than those in the SH during NH winter. A diagnostic evaluation of the patterns of correlation, the mesospheric zonal winds, and the stability criteria suggests that the temperature perturbations in the midlatitude summer mesosphere are more closely associated with the summer stratosphere directly below than with the wave activity in the winter stratosphere. This suggests that the interhemispheric coupling in the stratosphere is driving or contributing to the coupling between the winter stratosphere and the summer mesosphere that has been reported in several investigations.

Significance Statement

There are many instances in which one part of the atmosphere is found to regularly respond to perturbations occurring in a distant region. In this study, we use observations to investigate one such pattern: temperature changes at high altitude (60–100 km) in the summer that follow dynamical changes near the winter pole at 40–60 km. Such analysis is useful to understand which physical processes contribute to the global connectivity and variability of the atmosphere.
Effects of Sea Spray on Large-Scale Climatic Features over the Southern Ocean
Authors: Song Yajuan , Qiao Fangli , Liu Jiping , Shu Qi , Bao Ying , Wei Meng , Song Zhenya
Published: April 2022

The Southern Ocean, characterized by strong westerly winds and a rough sea state, exhibits the most pronounced sea spray effects. Sea spray ejected by ocean surface waves enhances heat and water exchange at the air–sea interface. However, this process has not been considered in current climate models, and the influence of sea spray on the coupled air–sea system remains largely unknown. This study incorporated a parameterization of the sea spray influence on latent and sensible heat fluxes into the First Institute of Oceanography Earth System Model version 2.0 (FIO-ESM v2.0), a climate model coupled with an ocean surface waves component. The results indicate that the spray-mediated enthalpy flux accounted for over 20%–50% of the total value. Sea spray promoted ocean evaporation and heat transport, resulting in air and ocean surface cooling and strengthened westerly winds. Furthermore, a moist and stable atmosphere favored an increase in cloud fraction over the Southern Ocean, particularly low-level clouds. Increased clouds reflected downward shortwave radiation and reduced solar radiation absorption at the surface. At present, the climate models participating in phase 6 of the Coupled Model Intercomparison Project (CMIP6) still suffer notable deficiencies in reasonably reproducing the climatological features of the Southern Ocean, including warm SST and underestimated clouds biases with more absorbed shortwave radiation. Our results suggest that consideration of sea spray effects is a feasible solution to mitigate these common biases and enhance the confidence in simulations and predictions with climate models.
Intraseasonal Oscillatory Modes in the Eastern Mediterranean Sea
Authors: Feliks Yizhak , Gildor Hezi , Mantel Nadav
Published: March 2022

The intraseasonal oscillations (ISOs) in sea currents in the eastern Mediterranean Sea near the central coast of Israel were analyzed by examining the velocity components of the sea currents at different depths as measured by acoustic Doppler current profilers located at various depths between 0 and 675 m. The total period covered by the observations was from December 2016 to May 2018. Prominent intraseasonal oscillations, much stronger than tidal velocity components, were observed in the upper part of the sea, at 30–70 m. The amplitudes of these oscillations are between 4 and 10 cm s−1 and their periods are 7, 11, 22, and 34–36 days. The strongest oscillations are found in the boreal winter. The ISOs in the sea currents were apparently induced by corresponding oscillations found in atmospheric wind velocity over the eastern Mediterranean at the surface and at 500 and 250 hPa, as suggested by the high correlations, 0.6–0.9, between the wind velocity components of the oscillatory modes in the atmosphere and the velocity component of the oscillatory modes in the sea currents with similar periods. We propose that the source of the ISOs in the atmosphere over the eastern Mediterranean is the South Asian jet wave train. The track of this wave train passes over the eastern Mediterranean, and the periods of the ISOs in the wave train are in the same band as the oscillations found here. The wave train is equivalently barotropic and strongest in the upper troposphere. This property of the wave train can explain the high correlation found between the oscillatory modes of wind velocity at 250 or 500 hPa and those in the sea currents. In all the cases besides the 7-day oscillatory mode, the significant oscillatory modes found at 250 or 500 hPa are also significant in the velocity components of the surface wind.
Why Mean Potential Vorticity Cannot Be Materially Conserved in the Eddying Southern Ocean
Authors: Stanley Geoffrey J. , Marshall David P.
Published: April 2022

Downstream of Drake Passage, the Antarctic Circumpolar Current (ACC) veers abruptly northward along the continental slope of South America. This spins down the ACC, akin to the western boundary currents of ocean gyres. During this northward excursion, the mean potential vorticity (PV) increases dramatically (decreases in magnitude) by up to a factor of 2 along mean geostrophic streamlines on middepth buoyancy surfaces. This increase is driven by drag near the continental slope, or by breaking eddies further offshore, and is balanced by a remarkably steady, eddy-driven decrease of mean PV along these northern circumpolar streamlines in the open ocean. We show how two related eddy processes that are fundamental to ACC dynamics—poleward buoyancy fluxes and downward fluxes of eastward momentum—are also concomitant with materially forcing PV to increase on the northern flank of a jet at middepth, and decrease on the southern flank. For eddies to drive the required mean PV decrease along northern streamlines, the ACC merges with the subtropical gyres to the north, so these streamlines inhabit the southern flanks of the combined ACC–gyre jets. We support these ideas by analyzing the time-mean PV and its budget along time-mean geostrophic streamlines in the Southern Ocean State Estimate. Our averaging formalism is Eulerian, to match the model’s numerics. The thickness-weighted average is preferable, but its PV budget cannot be balanced using Eulerian 5-day averaged diagnostics, primarily because the z-level buoyancy and continuity equations’ delicate balances are destroyed upon transformation into the buoyancy-coordinate thickness equation.

Significance Statement

The Antarctic Circumpolar Current is the world’s largest ocean current and a key controller of Earth’s climate. As the westerly winds that drive this current shift poleward under global warming, it is vital to know whether the current will follow. To begin addressing this, we study the current’s fundamental dynamics, and constraints, under present-day conditions. By analyzing angular momentum and stratification together, we show that the current is weakened near boundaries and strengthened by eddies elsewhere. The strengthening effects of eddies are isolated to the current by merging the current with oceanic gyres to the north. This gives a new perspective on why the current travels so far northward alongside South America, and may provide dynamical constraints on future changes.
Objective Verification of Clear-Air Turbulence (CAT) Diagnostic Performance in China Using In Situ Aircraft Observation
Authors: Hu Boyan , Ding Jinfeng , Liu Gang , Tang Jianping
Published: April 2022

This study analyzes the spatial and temporal distribution characteristics of the in situ aircraft observations in the middle and higher troposphere in 2019. These aircraft observations are mainly distributed in China, and relatively evenly recorded between 0000 and 1500 UTC in time and 6 and 10 km in height. Based on the 3395 stronger clear-air turbulence (CAT) events and 4038 weaker CAT events selected from the observations in the study region (15°–55°N, 70°–140°E), the performances of 24 CAT diagnostics calculated from the ERA5 data are evaluated. Results show that the diagnostics connected with vertical wind shear (i.e., version 1 of the North Carolina State University index, negative Richardson number, variant 3 and variant 1 of Ellrod’s turbulence index) have the best performances. However, the performances vary greatly from season to season, and overall performances are the best in winter and worst in summer. The annual and seasonal best thresholds for these diagnostics are also listed in this study.
Three-Dimensional Thermodynamic Observations in Supercell Thunderstorms from Swarms of Balloon-Borne Sondes
Authors: Bartos Elissa A. , Markowski Paul M. , Richardson Yvette P.
Published: March 2022

This study analyzes aboveground thermodynamic observations in three tornadic supercells obtained via swarms of small balloon-borne sondes acting as pseudo-Lagrangian drifters; the storm-relative winds draw the sondes through the precipitation, outflow, and baroclinic zones, which are believed to play key roles in tornado formation. Three-dimensional thermodynamic analyses are produced from the in situ observations. The coldest air is found at the lowest analysis levels, where virtual potential temperature deficits of 2–5 K are observed. Air parcels within the forward-flank outflow are inferred from their equivalent potential temperatures to have descended only a few hundred meters or less, whereas parcels within the rear-flank outflow are inferred to have downward excursions of 1–2 km. Additionally, the parcels following paths toward the low-level mesocyclone pass through horizontal buoyancy gradients that are strongest in the lowest 750 m and estimated to be capable of baroclinically generating horizontal vorticity having a magnitude of 6–10 × 10−3 s−1. A substantial component of the baroclinically generated vorticity is initially crosswise, though the vorticity subsequently could become streamwise given the leftward bending of the airstream in which the vorticity is generated. The baroclinically generated vorticity could contribute to tornado formation upon being tilted upward and stretched near the surface beneath a strong, dynamically forced updraft.

Significance Statement

Swarms of balloon-borne probes are used to produce the first-ever, three-dimensional mappings of temperature from in situ observations within supercell storms (rotating storms with high tornado potential). Temperature has a strong influence on the buoyancy of air, and horizontal variations of buoyancy generate spin about a horizontal axis. Buoyancy is one of the primary drivers of upward and downward motions in thunderstorms, and in supercell storms, horizontally oriented spin can be tipped into the vertical and amplified by certain arrangements of upward and downward motions. Unfortunately, the long-standing lack of temperature observations has hampered scientists’ ability to evaluate computer simulations and the tornadogenesis theories derived from them. We find that significant spin could be generated by the horizontal buoyancy variations sampled by the probes.
Optically controlled dual-band quantum dot infrared photodetector
Authors: Vichi Stefano , Bietti Sergio , Basset Francesco Basso , Tuktamyshev Artur , Fedorov Alexey , Sanguinetti Stefano
Published: March 2022

We present the design for a novel type of dual-band photodetector in the thermal infrared spectral range, the Optically Controlled Dual-band quantum dot Infrared Photodetector (OCDIP). This concept is based on a quantum dot ensemble with a unimodal size distribution, whose absorption spectrum can be controlled by optically injected carriers. An external pumping laser varies the electron density in the QDs, permitting to control the available electronic transitions and thus the absorption spectrum. We grew a test sample which we studied by AFM and photoluminescence. Based on the experimental data, we simulated the infrared absorption spectrum of the sample, which showed two absorption bands at 5.85 μm and 8.98 μm depending on the excitation power.
Local Tumor Behavior Associated With Survival Among Patients With Paraganglioma of the Head and Neck
Authors: Harley Randall J. , Lee Jason H. , Ostrander Benjamin T. , Finegersh Andrey , Pham Tammy B. , Tawfik Kareem O. , Ren Yin , Faraji Farhoud , Friedman Rick A.
Published: March 2022

Objective

The purpose of this study was to evaluate the utility of ICD‐O‐3–classified local tumor behavior as a prognosticator of head and neck paraganglioma (HNP) outcomes.

Study Design

Retrospective cohort study.

Setting

National Cancer Database between 2004 and 2016.

Methods

This study included patients aged ≥18 years who were diagnosed with HNP. Clinical outcomes and clinicopathologic features were compared with regard to local tumor behavior.

Results

Our study included 525 patients, of which the majority had HNP classified as locally invasive (45.9%) or borderline (37.9%). The most common anatomic sites involved were the carotid body (33.7%), intracranial regions (29.0%), or cranial nerves (25.5%). Carotid body tumors were exclusively locally invasive, whereas intracranial and cranial nerve HNP were overwhelmingly benign or borderline (94% and 91%, respectively). One‐fourth of patients underwent pathologic analysis of regional lymph nodes, of which the majority were positive for metastasis (80.6%). Metastasis to distant organs was twice as common in patients with locally invasive tumors vs benign (15% vs 7.1). For benign disease, surgery with radiotherapy (adjusted hazard ratio [aHR], 40.45; P =. 006) and active surveillance (aHR, 24.23; P =. 008) were associated with worse survival when compared with surgery alone. For locally invasive tumors, greater age (aHR, 1.07; P <. 0001) and positive surgical margins (aHR, 4.13; P =. 010) were predictors of worse survival, while combined surgery and radiotherapy were predictors of improved survival vs surgery alone (aHR, 0.31; P =. 027).

Conclusion

While criteria for tumor behavior could not be defined, our results suggest that such a classification system could be used to enhance HNP risk stratification and guide clinical management decisions.
ALGAEFUN with MARACAS, microALGAE FUNctional enrichment tool for MicroAlgae RnA-seq and Chip-seq AnalysiS
Authors: Romero-Losada Ana B. , Arvanitidou Christina , de los Reyes Pedro , García-González Mercedes , Romero-Campero Francisco J.
Published: March 2022

Background

Microalgae are emerging as promising sustainable sources for biofuels, biostimulants in agriculture, soil bioremediation, feed and human nutrients. Nonetheless, the molecular mechanisms underpinning microalgae physiology and the biosynthesis of compounds of biotechnological interest are largely uncharacterized. This hinders the development of microalgae full potential as cell-factories. The recent application of omics technologies into microalgae research aims at unraveling these systems. Nevertheless, the lack of specific tools for analysing omics raw data generated from microalgae to provide biological meaningful information are hampering the impact of these technologies. The purpose of ALGAEFUN with MARACAS consists in providing researchers in microalgae with an enabling tool that will allow them to exploit transcriptomic and cistromic high-throughput sequencing data.

Results

ALGAEFUN with MARACAS consists of two different tools. First, MARACAS (MicroAlgae RnA-seq and Chip-seq AnalysiS) implements a fully automatic computational pipeline receiving as input RNA-seq (RNA sequencing) or ChIP-seq (chromatin immunoprecipitation sequencing) raw data from microalgae studies. MARACAS generates sets of differentially expressed genes or lists of genomic loci for RNA-seq and ChIP-seq analysis respectively. Second, ALGAEFUN (microALGAE FUNctional enrichment tool) is a web-based application where gene sets generated from RNA-seq analysis as well as lists of genomic loci from ChIP-seq analysis can be used as input. On the one hand, it can be used to perform Gene Ontology and biological pathways enrichment analysis over gene sets. On the other hand, using the results of ChIP-seq data analysis, it identifies a set of potential target genes and analyses the distribution of the loci over gene features. Graphical representation of the results as well as tables with gene annotations are generated and can be downloaded for further analysis.

Conclusions

ALGAEFUN with MARACAS provides an integrated environment for the microalgae research community that facilitates the process of obtaining relevant biological information from raw RNA-seq and ChIP-seq data. These applications are designed to assist researchers in the interpretation of gene lists and genomic loci based on functional enrichment analysis. ALGAEFUN with MARACAS is publicly available on https://greennetwork.us.es/AlgaeFUN/.
ThPOK inhibits the immune escape of gastric cancer cells by inducing STPG1 to inactivate the ERK pathway
Authors: Chen Ying , Jiang Lili , Xia Lingli , Zhang Gang , Chen Lan
Published: April 2022

Background

Gastric cancer is the second most frequently diagnosed cancer worldwide. Weak immunogenicity helps cancer cells escape from immune elimination and grow into predominant subpopulations. This study aimed to investigate the effect of Zinc finger and BTB domain containing 7B (Zbtb7b, Alias ThPOK) on T cell activation after coculture with gastric cancer cells.

Methods

Cell Counting Kit-8 assay (CCK-8) was performed to explore the viability of gastric cancer cells. Flow cytometry analysis was used to measure CD3+ T cell proliferation and the ratio of activated IFN-γ+ T cells which were co-incubated with gastric cancer cells (HGC-27, SNU-1). The binding between ThPOK and the promoter of its target sperm tail PG-rich repeat containing 1 (STPG1) was explored using ChIP and luciferase reporter assays. Relative gene expression was quantified using RT-qPCR.

Results

ThPOK was expressed at a low level in gastric cancer tissues and cells at mRNA and protein levels. Gastric cancer patients with lower ThPOK expression had poorer prognosis. ThPOK overexpression suppressed gastric cancer cell viability and increased T cell activation. ThPOK served as a transcription factor for STPG1. STPG1 expression was also at a low level in the tissues and cells of gastric cancer. ThPOK positively regulated the mRNA and protein levels of STPG1 in gastric cancer cells. Moreover, ThPOK was demonstrated to bind with STPG1 promoter. STPG1 upregulation also exerted inhibitory effects on gastric cancer cell viability and T cell activation. Additionally, ThPOK and STPG1 were revealed to inactivate the ERK pathway in gastric cancer cells.

Conclusion

ThPOK inhibits gastric cancer cell viability and increases T cell activation by inducing STPG1 to inactivate the ERK pathway.
The gut bacterial microbiome of Nile tilapia (Oreochromis niloticus) from lakes across an altitudinal gradient
Authors: Bereded Negash Kabtimer , Abebe Getachew Beneberu , Fanta Solomon Workneh , Curto Manuel , Waidbacher Herwig , Meimberg Harald , Domig Konrad J.
Published: April 2022

Background

Microorganisms inhabiting the gut play a significant role in supporting fundamental physiological processes of the host, which contributes to their survival in varied environments. Several studies have shown that altitude affects the composition and diversity of intestinal microbial communities in terrestrial animals. However, little is known about the impact of altitude on the gut microbiota of aquatic animals. The current study examined the variations in the gut microbiota of Nile tilapia (Oreochromis niloticus) from four lakes along an altitudinal gradient in Ethiopia by using 16S rDNA Illumina MiSeq high-throughput sequencing.

Results

The results indicated that low-altitude samples typically displayed greater alpha diversity. The results of principal coordinate analysis (PCoA) showed significant differences across samples from different lakes. Firmicutes was the most abundant phylum in the Lake Awassa and Lake Chamo samples whereas Fusobacteriota was the dominant phylum in samples from Lake Hashengie and Lake Tana. The ratio of Firmicutes to Bacteroidota in the high-altitude sample (Lake Hashengie, altitude 2440 m) was much higher than the ratio of Firmicutes to Bacteroidota in the low altitude population (Lake Chamo, altitude 1235 m). We found that the relative abundances of Actinobacteriota, Chloroflexi, Cyanobacteria, and Firmicutes were negatively correlated with altitude, while Fusobacteriota showed a positive association with altitude. Despite variability in the abundance of the gut microbiota across the lakes, some shared bacterial communities were detected.

Conclusions

In summary, this study showed the indirect influence of altitude on gut microbiota. Altitude has the potential to modulate the gut microbiota composition and diversity of Nile tilapia. Future work will be needed to elucidate the functional significance of gut microbiota variations based on the geographical environment.

Significance and impact of the study

Our study determined the composition and diversity of the gut microbiota in Nile tilapia collected from lakes across an altitude gradient. Our findings greatly extend the baseline knowledge of fish gut microbiota in Ethiopian lakes that plays an important role in this species sustainable aquaculture activities and conservation.
Association between post-extubation upper airway obstruction symptoms and airway size measured by computed tomography: a single-center observational study
Authors: Shinohara Mafumi , Iwashita Masayuki , Abe Takeru , Takeuchi Ichiro
Published: March 2022

Background

Computed tomography (CT) is often performed to assess patients; however, little is known about how airway size measured by CT scan imaging might influence the occurrence of post-extubation upper airway obstruction.

Methods

This study aimed to evaluate the association between airway size measured by CT and the incidence of post-extubation upper airway obstruction symptoms for each sex. This single-center observational study was conducted at a tertiary emergency medical center/severe trauma center with a 12-bed intensive care unit. We enrolled consecutive adult patients (aged ≥ 20 years), who were intubated in the emergency room, between January 2016 and March 2019. Patients who underwent a CT scan of the glottic region within three hours before and after intubation were included in the analysis. For each sex, we first divided the patients into two groups: those who had post-extubation stridor, hoarseness, or both and those who had no such symptoms. Then, we compared the two groups using the Mann–Whitney U test and Fisher’s exact test. Univariate and multivariate logistic regression analyses were also performed.

Results

During the 39 months, 855 patients were enrolled in this study. A total of 217 patients underwent CT of the glottic region within three hours before and after intubation. Five patients had no records of symptoms after extubation. Thus, we analyzed data from 212 patients. This study included 144 males and 68 females. In female patients, the median [inter-quartile range] (average) of the transverse diameter of the glottis/endotracheal tube outer diameter (OD) ratio was smaller in patients with post-extubation upper airway obstruction symptoms than in patients without the symptoms (1.00 [1.00–1.00] (0.9572) vs. 1.00 [1.00–1.00] (1.00296), respectively; p = .013). Multivariate logistic regression analysis showed that the glottis/tube OD ratio < 1 was associated with the symptoms in females (odds ratio: 95% confidence interval, 5.68: 1.04–30.97). There was no relation between the airway sizes and the symptoms in male patients.

Conclusions

In female patients, no gap between the endotracheal tube and the vocal codes or the glottic transverse diameter being smaller than the endotracheal tube OD on CT scan was associated with post-extubation upper airway obstruction symptoms.
Profile and dynamics of infectious diseases: a population-based observational study using multi-source big data
Authors: Zhao Lin , Wang Hai-Tao , Ye Run-Ze , Li Zhen-Wei , Wang Wen-Jing , Wei Jia-Te , Du Wan-Yu , Yin Chao-Nan , Wang Shan-Shan , Liu Jin-Yue , Ji Xiao-Kang , Wang Yong-Chao , Cui Xiao-Ming , Liu Xue-Yuan , Li Chun-Yu , Qi Chang , Liu Li-Li , Li Xiu-Jun , Xue Fu-Zhong , Cao Wu-Chun
Published: April 2022

Background

The current surveillance system only focuses on notifiable infectious diseases in China. The arrival of the big-data era provides us a chance to elaborate on the full spectrum of infectious diseases.

Methods

In this population-based observational study, we used multiple health-related data extracted from the Shandong Multi-Center Healthcare Big Data Platform from January 2013 to June 2017 to estimate the incidence density and describe the epidemiological characteristics and dynamics of various infectious diseases in a population of 3,987,573 individuals in Shandong province, China.

Results

In total, 106,289 cases of 130 infectious diseases were diagnosed among the population, with an incidence density (ID) of 694.86 per 100,000 person-years. Besides 73,801 cases of 35 notifiable infectious diseases, 32,488 cases of 95 non-notifiable infectious diseases were identified. The overall ID continuously increased from 364.81 per 100,000 person-years in 2013 to 1071.80 per 100,000 person-years in 2017 (χ2 test for trend, P < 0.0001). Urban areas had a significantly higher ID than rural areas, with a relative risk of 1.25 (95% CI 1.23–1.27). Adolescents aged 10–19 years had the highest ID of varicella, women aged 20–39 years had significantly higher IDs of syphilis and trichomoniasis, and people aged ≥ 60 years had significantly higher IDs of zoster and viral conjunctivitis (all P < 0.05).

Conclusions

Infectious diseases remain a substantial public health problem, and non-notifiable diseases should not be neglected. Multi-source-based big data are beneficial to better understand the profile and dynamics of infectious diseases.
Evaluation of the SMARTCHEK Genesystem RT-qPCR assay for the detection of SARS-CoV-2 in clinical samples
Authors: Flores-León Diana , Quino Willi , Cúneo Alejandra E. , Caro-Castro Junior , Cucho-Meza Evans , Gavilan Ronnie G.
Published: April 2022

Background

The COVID-19 pandemic remains the main public health problem, due to the quick and easy dissemination of the causal agent, SARS-CoV-2 virus, around the world. Since the beginning of the pandemic, an opportune laboratory diagnosis has been critical to respond this emergency, and RT-qPCR has been used as reference molecular tests for detection of SARS-CoV-2.

Methods

In this study, we performed the evaluation of a RT-qPCR SMARTCHEK platform (SMARTCHEK, Genesystem) for SARS-CoV-2 detection based on the amplification of RdRp and N gene markers. The platform was evaluated with nasopharyngeal swab samples corresponding to 360 suspected cases of COVID-19 which were remitted to Instituto Nacional de Salud in Peru. This quick method was compared with conventional RT-qPCR as gold standard.

Results

The RT-qPCR SMARTCHEK showed a 98.1% sensitivity (CI: 93.3–99.8%), a 98.8% specificity (CI: 96.6–99.8%), a 97.2% positive predictive value (CI: 92–99.4%) and a 99.2% negative predictive value (CI: 97.2—99.9%). The assay demonstrated a strong agreement between the RT-qPCR SMARTCHEK and conventional RT-qPCR (kappa value ≥ 0.966).

Conclusion

The RT-qPCR SMARTCHEK is a platform that gives reliable and fast results, with high sensitivity and specificity for the detection of SARS-CoV-2, and it will be considered a suitable alternative to COVID-19 diagnosis in low-resource settings.
Parental distress rating at the child’s age of 15 years predicts probable mental diagnosis: a three-year follow-up
Authors: Carlén Kristina , Suominen Sakari , Augustine Lilly , Saarinen Maiju M. , Aromaa Minna , Rautava Päivi , Sourander André , Sillanpää Matti
Published: April 2022

Background

Mental health in adolescence is an increasing global public health concern. Over half of all mental disorders debut by 14 years of age and remain largely untreated up to adulthood, underlining the significance of early detection. The study aimed to investigate whether parental distress rating at the child’s age of 15 predicts a probable mental diagnosis in a three-year follow-up.

Methods

All data was derived from the Finnish Family Competence (FFC) Study. The analysis focused on whether parental CBCL (Child Behavior Checklist) rating (n = 441) at the child’s age of 15 years predicted the outcome of the child’s standardised DAWBA (Development and Well-Being Assessment) interview at offspring’s 18 years.

Results

Multivariable analysis showed that a one-unit increase in the total CBCL scores increased the relative risk of a DAWBA-based diagnosis by 3% (RR [95% CI] 1.03 [1.02–1.04], p < 0.001).

Conclusions

Parental CBCL rating in a community sample at the adolescent’s age of 15 contributes to early identification of adolescents potentially at risk and thus benefitting from early interventions.
Fatal noncardiogenic pulmonary edema related to nonionic, iso-osmolar iodine contrast medium: one case report
Authors: Yi Kun-ming , Li Xue
Published: March 2022

Background

Noncardiogenic pulmonary edema (NCPE) is a rare and life-threatening allergy-like reaction to the intravascular injection of a nonionic radiographic agent. We first describe a very rare case of fatal NCPE after the intravenous injection of nonionic, iso-osmolar iodine contrast media.

Case presentation

A 55-year-old male patient was admitted to the hospital with esophageal cancer. After the intravenous administration of 100 mL iodixanol, the patient first exhibited digestive tract symptoms, including abdominal pain, diarrhea, and vomiting, with no dyspnea, rash, itching, or throat edema. He received anti-allergy treatment, but his symptoms did not improve; instead, he further developed pulmonary edema. Arterial blood gas analysis results were as follows: pH, 7.08; PO2, 70 mm Hg; PCO2, 40 mm Hg; and SaO2, 52%. Then, the patient received emergent tracheal intubation and ventilation to assist breathing, and he was transferred to the intensive care unit (ICU) for further treatment. In the ICU, the patient developed shock and respiratory and circulatory failure; therefore, he received shock resuscitation, acidosis correction, muscle relaxants to lower the work of breathing, and cardiotonic therapy. The patient eventually died. During the ICU period, emergency bedside color ultrasound showed a diffuse B line in both lungs, and the size of the cardiac cavity was normal, but the ventricular rate was extremely fast. Chest radiography showed pulmonary edema with a normal cardiac silhouette, and the brain natriuretic peptide (BNP) level was in the normal range.

Conclusions

NCPE is a rare and critical allergy-like reaction to the use of a nonionic iso-osmolar radiocontrast contrast medium. Clinicians should pay very close attention to digestive tract manifestations during the medical observation of patients, as gastrointestinal manifestations may be the prodromal symptoms of NCPE caused by iso-osmolar contrast medium injection.
Knee varus alters three-dimensional ankle alignment in standing- a study with upright computed tomography
Authors: Hakukawa Satoshi , Kaneda Kazuya , Oki Satoshi , Harato Kengo , Yamada Yoshitake , Niki Yasuo , Nagura Takeo , Nakamura Masaya , Jinzaki Masahiro
Published: April 2022

Background

For knee osteoarthritis (OA) treatment, it is important to correct the lower limb alignment including the foot. However, in the upright position, lower limb alignment is generally assessed from the body surface or radiographs, and it is a challenge to capture the exact characteristics of three-dimensional lower limb alignment. The purpose of the study was to measure lower limb alignment in patients with knee OA using upright computed tomography (CT) and radiography, and to identify features of knee joint deformity.

Methods

A total of 45 limbs in 25 patients with knee OA were enrolled. The subjects underwent both upright CT and radiography for the whole lower limb in the standing position. The joint angles were calculated on both images. The degree of knee OA was classified according to Kellgren-Lawrence (KL) grade by referring to radiography, which is mainly based on the degree of articular cartilage loss and severity of osteophytes, and the characteristics or correlation between knee and ankle joint in each group was investigated.

Results

In KL-I, there was an association between varus of the knee joint and internal rotation of the talocrural joint (r = 0.76, P < 0.05). In KL-II, there was an association between varus of the knee joint and eversion of the subtalar joint (r = 0.63, P < 0.05) and talocrural joint (r = − 0.65, P < 0.05). In KL-III, there was an association between varus of the knee joint and internal rotation of the subtalar joint (r = − 0.62, P < 0.05), and in KL-IV, there was an association between varus of the knee joint and internal rotation of the subtalar joint (r = − 0.58, P < 0.05).

Conclusions

The lower limb alignment of patients with knee OA in the standing position was found that as knee OA worsened, it became apparent that compensatory knee joint alignment depended on the ankle joint rather than the subtalar joint. The results may help in the rehabilitation of patients with knee OA, since the ankle joint alignment has a significant impact on the knee joint during coarse movements involving load.
Protocol for a multicenter prospective cohort study evaluating arthroscopic and non-surgical treatment for microinstability of the hip joint
Authors: Öhlin Axel , Senorski Eric Hamrin , Sansone Mikael , Leff Gretchen , Desai Neel , Lindman Ida , Ayeni Olufemi R. , Safran Marc R.
Published: March 2022

Background

Microinstability of the hip joint is a proposed cause of hip pain and reduced function in young individuals. The underlying mechanism is thought to be extraphysiological hip motion due to bony deficiency and/or soft tissue deficiency or decreased soft tissue function. Recently, the condition has gained increased attention, and despite the fact that treatment today includes both non-surgical and surgical approaches, there is limited evidence on diagnostic specificity and treatment effects. The aim of this study is to evaluate clinical outcomes of both non-surgical and surgical treatment for microinstability of the hip joint.

Methods

A multicenter prospective cohort study is planned to evaluating the outcome of physical therapy aimed at stabilizing the hip joint, as well as arthroscopic plication of the hip joint capsule, if the physical therapy fails. Outcomes will be evaluated using hip-specific patient-reported outcome measures: the short version of the International Hip Outcome Tool and the Copenhagen Hip and Groin Outcome Score, strength and function tests, health-related quality of life as determined using the European Quality of Life-5 Dimensions and the European Quality of Life-Visual Analog Scale, sports activity levels according to the Hip Sport Activity Scale, and reported complications. Patients will be evaluated at 6, 12 and 24 months after each treatment.

Discussion

It is important to evaluate the clinical outcomes of both non-surgical and surgical treatment for suspected microinstability of the hip joint, and the planned prospective evaluation will contribute to the understanding of non-surgical as well as surgical treatment outcomes, including complications.

Trial registration

Clinicaltrials.gov: NCT04934462. Registered June 22 2021.
Women survivors of intimate partner violence talk about using e-health during pregnancy: a focus group study
Authors: Fernández López Rodrigo , de-León-de-León Sabina , Martin-de-las-Heras Stella , Torres Cantero Juan Carlos , Megías Jesús L. , Zapata-Calvente Antonella Ludmila
Published: March 2022

Background

Pregnancy is a period of particular vulnerability to experience intimate partner violence against women (IPVAW). eHealth strategies have been implemented to identify women exposed to IPVAW and to combat the abuse and empower them, but there is a lack of evidence on the use of these strategies among pregnant women. This work aims to identify the needs, concerns and preferences of survivors about the use of eHealth strategies to counsel and empower pregnant victims of IPVAW in antenatal care.

Methods

A focus group of six IPVAW survivors who had been pregnant was conducted and open questions about the use of eHealth strategies were asked. The session was recorded, transcribed and thematically analyzed. We identified three main themes: needs and worries of pregnant women experiencing IPVAW, key aspects of video counseling sessions and usefulness of safety planning apps.

Results

Women highlighted the relevant role of healthcare professionals—especially midwives—in the identification of IPVAW and the wellbeing of their children as one of the main concerns. They perceived video counseling and safety planning apps as valuable resources. The preferred contents for a video counseling intervention were awareness-raising of the situation, self-esteem and legal advice. They also proposed safety and pregnant-related aspects that should be taken into account in the design of the video counseling sessions and the safety planning app.

Conclusions

Video counseling sessions and safety planning apps are potentially useful tools to counsel and empower women who experience IPVAW during pregnancy. Midwives play a key role in this endeavor.
Problem-based or lecture-based learning, old topic in the new field: a meta-analysis on the effects of PBL teaching method in Chinese standardized residency training
Authors: Li Tingting , Wang Weidong , Li Zhijie , Wang Hongmiao , Liu Xiaodan
Published: March 2022

Background

Standardized residency training (SRT) is crucial for graduate medical education and the training of high-quality doctors. Nevertheless, China started SRT nationwide only in the recent decade. During these years, researchers have been searching for suitable teaching methods to improve the abilities of residents. Although the problem-based learning (PBL) teaching mode has been applied in undergraduate teaching for many years, the teaching effect of PBL has not been unified in Chinese SRT according to the core competences of the residents.

Methods

Studies that compared the teaching effect of PBL and lecture-based learning (LBL) on SRT in China from January 2010 to April 2020 in the Chinese databases, such as China National Knowledge Infrastructure (CNKI), WanFang, WeiPu, Chinese BioMedical Literature (CBM), and English-language online databases, such as PubMed, Embase, and Cochrane Library were systematically reviewed. Data were analyzed using the Stata version 12.0 software.

Results

A total of 75 articles (76 studies) were included in this meta-analysis. Compared with LBL group, PBL-based methods are more effective in the mastery of medical theory knowledge (WMD = 7.14, 95% CI: 5.93–8.34), operational skills (WMD = 6.54, 95% CI: 4.55–8.53), analysis and diagnosis of cases (WMD = 8.52, 95% CI: 7.50–9.53), and overall capacity (WMD = 8.70, 95% CI: 6.87–10.53), but showed no advantage on operational skills in diagnostic imaging (WMD = 1.30, 95% CI: -0.11–2.71). The questionnaire surveys analyzed in this meta-analysis indicated the positive effects of PBL on the mastery of theoretical knowledge, clinical diagnostic thinking, teamwork ability, ability to analyze and solve problems, ability to consult documents, learning interest and learning efficiency, but that there were no advantages in improving self-directed learning ability, communication ability and hands-on ability. The questionnaire result analyzed in this meta-analysis also showed the residents’ satisfaction with PBL-based strategies.

Conclusions

Taken together, the current meta-analysis provides a systematic and comprehensive analysis on PBL teaching mode in Chinese SRT and outlines a path for further research on the detailed design of suitable teaching methods for different specialties and abilities.
Diffusion of an innovation: growth in video capsule endoscopy in the U.S. Medicare population from 2003 to 2019
Authors: Read Andrew J. , Rice Michael D. , Baker Jason R. , Waljee Akbar K. , Saini Sameer D.
Published: March 2022

Background

Video capsule endoscopy (VCE), approved by the U.S. Food and Drug Administration (FDA) in 2001, represented a disruptive technology that transformed evaluation of the small intestine. Adoption of this technology over time and current use within the U.S. clinical population has not been well described.

Methods

To assess the growth of capsule endoscopy within the U.S. Medicare provider population (absolute growth and on a population-adjusted basis), characterize the providers performing VCE, and describe potential regional differences in use. Medicare summary data from 2003 to 2019 were used to retrospectively analyze capsule endoscopy use in a multiple cross-sectional design. In addition, detailed provider summary files were used from 2012 to 2018 to characterize provider demographics.

Results

VCE use grew rapidly from 2003 to 2008 followed by a plateau from 2008 to 2019. There was significant variation in use of VCE between states, with up to 10-fold variation between states (14.6 to 156.1 per 100,000 enrollees in 2018). During this time, the adjusted VCE use on a population-adjusted basis declined, reflecting saturation of growth.

Conclusions

Growth of VCE use over time follows an S-shaped diffusion of innovation curve demonstrating a successful diffusion of innovation within gastroenterology. The lack of additional growth since 2008 suggests that current levels of use are well matched to overall population need within the constraints of reimbursement. Future studies should examine whether this lack of growth has implications for access and healthcare inequities.