bluuebunny/crossref_metadata_2025 huggingface dataset:
Deuteration Effects on the Transport Properties of (TMTTF)2X Salts
Authors: Rohwer Andrea , Dressel Martin , Nakamura Toshikazu
Published: November 2020

The electronic properties in the quasi-one-dimensional Fabre salts are strongly affected by electronic correlations along the molecular stacks, but also by the interactions with the anions located in a cage that is formed by the methyl end groups. We systematically compare the charge transport in deuterated and protonated (TMTTF)2X salts with the anions X = Br, PF6, SbF6, and ClO4, ranging from Mott and Efros–Shklovskii variable-range hopping to activated band transport with a temperature dependent energy gap. The strong dependence of charge localization and ordering on the anion size and deuteration confirms the subtle structural involvement of the anions in the charge transport along the TMTTF stack.
Computational Approach to Dynamic Systems through Similarity Measure and Homotopy Analysis Method for Renewable Energy
Authors: Khan Noor Saeed , Kumam Poom , Thounthong Phatiphat
Published: November 2020

To achieve considerably high thermal conductivity, hybrid nanofluids are some of the best alternatives that can be considered as renewable energy resources and as replacements for the traditional ways of heat transfer through fluids. The subject of the present work is to probe the heat and mass transfer flow of an ethylene glycol based hybrid nanofluid (Au-ZnO/C2H6O2) in three dimensions with homogeneous-heterogeneous chemical reactions and the nanoparticle shape factor. The applications of appropriate similarity transformations are done to make the corresponding non-dimensional equations, which are used in the analytic computation through the homotopy analysis method (HAM). Graphical representations are shown for the behaviors of the parameters and profiles. The hybrid nanofluid (Au-ZnO/C2H6O2) has a great influence on the flow, temperature, and cubic autocatalysis chemical reactions. The axial velocity and the heat transfer increase and the concentration of the cubic autocatalytic chemical reactions decreases with increasing stretching parameters. The tangential velocity and the concentration of cubic autocatalytic chemical reactions decrease and the heat transfer increases with increasing Reynolds number. A close agreement of the present work with the published study is achieved.
Transverse Micro Radiography Analysis of the Effect of Experimental Calcium-Containing Primer System on Demineralized Enamel
Authors: Koshimitsu Yusuke , Inoue Go , Sayed Mahmoud , Saad Amr , Ikeda Masaomi , Tagami Junji
Published: November 2020

Background: Enamel mineral density is dependent on a balanced dynamic process of demineralization and remineralization. Objective: We evaluated the remineralization potential of experimental calcium- containing primer (CaP) application on enamel subsurface lesions. Methods: Demineralized enamel samples obtained from bovine incisor teeth were prepared and cut buccolingually. All samples were divided into 4 main groups according to the type of primer: CLEARFIL SE BOND 2 Primer (SEP), experimental calcium-containing primer (CaP) and bond: CLEARFIL SE BOND 2 Bond (SEB), CLEARFIL Protect Bond (PBB) treatment: (1) SEP-SEB (2) SEP-PBB (3) CaP-SEB (4) CaP-PBB. Each group was subdivided according to storage time in artificial saliva: immediately, 1 month and 6 months. Then, the samples were analyzed using transverse micro radiography (TMR). Additionally, the samples treated with each primer were prepared for scanning electron microscope (SEM) and energy dispersive X-ray spectroscopy (EDS) observation. Two-way ANOVA was used for the statistical analysis of mineral loss (ΔZ: vol%·µm). Results: CaP-PBB had the significantly lowest ΔZ at all storage times, while SEP-SEB had the highest ΔZ (p < 0.05). SEM observations revealed that new crystals were formed on the surface after 6 months, and the ratio of calcium on the enamel increased in the EDS analysis. Conclusion: Experimental calcium-containing primer can provide additional mineral deposition, with even further deposition when combined with a fluoride-containing bond.
Postmortem Protein Degradation as a Tool to Estimate the PMI: A Systematic Review
Authors: Zissler Angela , Stoiber Walter , Steinbacher Peter , Geissenberger Janine , Monticelli Fabio C. , Pittner Stefan
Published: November 2020

Objectives: We provide a systematic review of the literature to evaluate the current research status of protein degradation-based postmortem interval (PMI) estimation. Special attention is paid to the applicability of the proposed approaches/methods in forensic routine practice. Method: A systematic review of the literature on protein degradation in tissues and organs of animals and humans was conducted. Therefore, we searched the scientific databases Pubmed and Ovid for publications until December 2019. Additional searches were performed in Google Scholar and the reference lists of eligible articles. Results: A total of 36 studies were included. This enabled us to consider the degradation pattern of over 130 proteins from 11 different tissues, studied with different methods including well-established and modern approaches. Although comparison between studies is complicated by the heterogeneity of study designs, tissue types, methods, proteins and outcome measurement, there is clear evidence for a high explanatory power of protein degradation analysis in forensic PMI analysis. Conclusions: Although only few approaches have yet exceeded a basic research level, the current research status provides strong evidence in favor of the applicability of a protein degradation-based PMI estimation method in routine forensic practice. Further targeted research effort towards specific aims (also addressing influencing factors and exclusion criteria), especially in human tissue will be required to obtain a robust, reliable laboratory protocol, and collect sufficient data to develop accurate multifactorial mathematical decomposition models.
A/T/(N) Profile in Cerebrospinal Fluid of Parkinson’s Disease with/without Cognitive Impairment and Dementia with Lewy Bodies
Authors: Bellomo Giovanni , Paolini Paoletti Federico , Chipi Elena , Petricciuolo Maya , Simoni Simone , Tambasco Nicola , Parnetti Lucilla
Published: November 2020

Neuropathological investigations report that in synucleinopathies with dementia, namely Parkinson’s disease (PD) with dementia (PDD) and dementia with Lewy bodies (DLB), the histopathological hallmarks of Alzheimer’s Disease (AD), in particular amyloid plaques, are frequently observed. In this study, we investigated the cerebrospinal fluid (CSF) AD biomarkers in different clinical phenotypes of synucleinopathies. CSF Aβ42/Aβ40 ratio, phosphorylated tau and total tau were measured as markers of amyloidosis (A), tauopathy (T) and neurodegeneration (N) respectively, in 98 PD (48 with mild cognitive impairment, PD-MCI; 50 cognitively unimpaired, PD-nMCI), 14 PDD and 15 DLB patients, and 48 neurological controls (CTRL). In our study, CSF AD biomarkers did not significantly differ between CTRL, PD-MCI and PD-nMCI patients. In PD-nMCI and PD-MCI groups, A-/T-/N- profile was the most represented. Prevalence of A+ was similar in PD-nMCI and PD-MCI (10% and 13%, respectively), being higher in PDD (64%) and in DLB (73%). DLB showed the lowest values of Aβ42/Aβ40 ratio. Higher total tau at baseline predicted a worse neuropsychological outcome after one year in PD-MCI. A+/T+, i.e., AD-like CSF profile, was most frequent in the DLB group (40% vs. 29% in PDD).
Prevention of Children’s Burnout at School through the Tutoring and Guidance Process. A Structural Equation Model Analysis
Authors: Expósito-López Jorge , Chacón-Cuberos Ramón , Romero-Díaz de la Guardia José Javier , Parejo-Jiménez Noelia , Rodríguez-Fernández Sonia , Estrada-Vidal Ligia Isabel
Published: November 2020

The school burnout of children, defined as physical and mental exhaustion due to a lack of adjustment to the educational context, constitutes a serious problem in contemporary education. Thus, the determination of the elements that influence it and the possible strategies for avoiding it are key in the process of improving children’s well-being. A descriptive and cross-sectional study was conducted with a sample of 569 children aged 8 to 13 at eight primary education schools in Granada (Spain). With the aim of examining the association between the possible exhaustion of children and planned tutoring and guidance activities, a structural equation model (SEM) as the analytical technique was used. The results show good fit indices for the model (comparative fit index (CFI) = 0.955; normalised fit index (NFI) = 0.956; incremental fit index (IFI) = 0.946; root mean squared error approximation (RMSEA) = 0.089), which reflects the need for tutoring and guidance activities that are infused throughout the entire teaching process in order to preserve children’s well-being.
Let’s Get Engaged: The Nexus between Digital Technologies, Engagement and Learning
Authors: Kearney Sean , Maakrun Julie
Published: November 2020

The nexus between digital technologies, engagement and learning is often cited as being correlational, but is widely misunderstood. Research is clear that engagement can lead to better academic outcomes and improved learning, but less clear about the role of digital technologies in engaging students and the impact of digital technologies on student learning. Recent research suggests that in light of the increase in digital technologies in education that we must recognize the inherent challenges in increased use of digital technologies. In this article a small-scale case study was conducted to ascertain the level of student engagement and distraction during lectures. The study was repeated for two different cohorts of teacher education students to establish consistency of results. The authors sought to determine the level of academic engagement by having students record the number of digital distractions they encountered throughout the lecture. The students self-reported their distractions during a ~100 min lecture. The researchers found consistency between the two cohorts when looking at the number of self-reported distractions per student. The findings suggest that technology was a major distraction and further could be seen, due to the number of distractions, to be causing disengagement. In this article the authors use this case study to start a conversation about the possible perils of digital technologies with regard to engagement and learning and what this means in a future that may be dictated by technology-enhanced learning.
DMRVR: Dynamic Milk-Run Vehicle Routing Solution Using Fog-Based Vehicular Ad Hoc Networks
Authors: Adriano Daniel Domingos , Montez Carlos , Novaes Antonio G. N. , Wangham Michelle
Published: November 2020

Milk-run tours with time windows are an essential strategy to collect goods to minimize production and transportation costs. Due to unexpected events at the supplier production or traffic congestion, delays can occur during the vehicle route execution, causing non-compliance between the logistics operator and the company. This paper describes the DMRVR (Dynamic Milk-Run Vehicle Routing) solution that uses a dynamic routing algorithm along with fog-based vehicular ad hoc networks for implementing the collection of goods in milk-run operations that respect the company’s time window. When a production delay occurs, the supplier sends a message through the vehicular network to alert the pickup vehicle, forcing it to make dynamic route changes to satisfy the constraints. We have implemented a queue with a timeout and retransmission features to improve the vehicular network’s message delivery. To assess the DMRVR solution, we analyzed the efficiency of the dynamic vehicle routing and the vehicular network impacts. In the experiments, we used an event-based network simulator OMNeT++ bidirectionally coupled with SUMO (Simulation of Urban Mobility), aiming to make the most realistic simulations. Simulation results show the average route time was lower than the time limit imposed by the company with the DMRVR solution. In dense vehicular network scenarios, the message delivery success rate is higher. Conversely, when the vehicular network scenario is sparse, it is necessary to balance network coverage and distribute more RSUs in specific places.
Online Prediction of Vehicular Fuel Cell Residual Lifetime Based on Adaptive Extended Kalman Filter
Authors: Song Ke , Wang Yimin , Hu Xiao , Cao Jing
Published: November 2020

The limited lifetime of proton exchange membrane fuel cell (PEMFC) inhibits the further development of the fuel cell industry. Prediction is one of the most effective means for managing the lifetime of a fuel cell because it can assist in the implementation of mitigation actions before a vehicular fuel cell fails by estimating the residual lifetime. Therefore, this study aimed to develop a PEMFC lifetime prediction method for online applications. This paper presents the online prediction method developed for the residual lifetime of a vehicular fuel cell, which utilises data processing with an adaptive extended Kalman filter and a prediction formula. The formula considers different operating conditions and the external environment, which is in accord with the actual operating conditions of fuel cell vehicles. This method realises the online prediction of the residual lifetime of a vehicular fuel cell by updating weight coefficients for the operating conditions and environmental factors. This prediction method was validated and analysed using a simulation. The influences of key parameters on the stability and prediction accuracy of the algorithm were evaluated. The prediction method proposed in this paper can provide a reference for studies on fuel cell lifetime prediction.
Food Security in the Context of Liquid Biofuels Production
Authors: Kurowska Krystyna , Marks-Bielska Renata , Bielski Stanisław , Kryszk Hubert , Jasinskas Algirdas
Published: November 2020

A crucial factor that determines the development of production and consumption markets for biofuels is the choice of raw materials that can ensure the highest possible production efficiency, the lowest cost and the smallest emission of harmful substances to the atmosphere during all production stages. Considerations underlying the development of biofuel production have been discussed as well as the theoretical mechanisms linking the generation of biofuels to the level of production and the variability of prices of agricultural raw products. The aim of this study has been to identify the scale at which energy raw materials originating from agriculture are used for liquid biofuels production and to explore their impact on food security. The study used public statistical data (OECD-FAO and IndexMundi). The time span of the analysis was from 2005 to 2018. First-generation biofuels based on food raw materials (cereal grains, root crops, sugarcane and vegetable oils) are becoming increasingly competitive with food production recent years have been a period of the dynamic growth in production of liquid biofuels. In 2018, the global production of these substances reached 167.9 billion litres (bioethanol and biodiesel together), consuming 16.1% of maize grain, 1.7% of wheat grain, 3.3% of grain of other feed grains and 13.5% of vegetable oil.
Techno-Economic Analysis of Glazed, Unglazed and Evacuated Tube Solar Water Heaters
Authors: Raza Syed Ali , Ahmad Syed Sulman , Ratlamwala Tahir Abdul Hussain , Hussain Ghulam , Alkahtani Mohammed
Published: November 2020

In the residential sector, a great part of the electricity goes into heating water by electric water heaters, which results in high CO2 emissions as well as an unprecedented increase in electricity demand leading to an energy crisis. This study offers solar water heaters as a more economical and efficient replacement for electric water heaters. The present study is aimed at investigating the potential for solar water heaters in the subcontinent, especially in India. A feasibility analysis is performed for seven cities in the subcontinent, namely Mumbai, New Delhi, Kolkata, Assam, Gujrat and Madhya Pradesh. Simulated results are based on a solar fraction, equity payback, multiple collectors (one, two or three), horizontal and tilted, type of collector (evacuated tube, glazed and unglazed), electricity saved and greenhouse gas (GHG) emission reduction. The collector’s area is made such that it should receive at least 50% of the solar fraction. The range of payback period varies from five to 15 years. The results show Gujrat being the most ideal site for solar water heaters.
To What Extent Is Manure Produced, Distributed, and Potentially Available for Bioenergy? A Step toward Stimulating Circular Bio-Economy in Poland
Authors: Sefeedpari Paria , Pudełko Rafał , Jędrejek Anna , Kozak Małgorzata , Borzęcka Magdalena
Published: November 2020

Bioenergy production from animal waste can be a key driver to achieving bio-economy goals. Developing a bio-economy sector could help to create opportunities for a circular system where not only people and the planet will be benefited, but it will also provide economic profitability to farmers, especially in the post-Covid period. To this end, manure production, its nutrient content, and bioenergy potential were estimated, along with their spatial distribution in the Lubelskie province, Poland. Farm-level data were processed and aggregated at the municipality level. Material balance equations were used to calculate the theoretical potential of livestock manure and bioenergy for different use scenarios: (1) Baseline (BC): direct manure application to land, which was compared against (2) Anaerobic Digestion (AD): anaerobic digestion to biogas with digestate returned to the fields (3) AD + Separation (AD + Sep): mechanical separation followed by anaerobic digestion, and (4) Surplus + AD: surplus manure (after application to the fields) is sent to anaerobic digestion. Manure, biogas, electricity, and thermal energy production of the AD scenario were estimated to be 7.5 Mt y−1, 378 Mm3 y−1, 907 GWe y−1, and 997.8 GWth y−1, respectively. The scenario, including mechanical separation followed by anaerobic digestion (AD + Sep), contributed to avoiding emissions to the largest extent (1 Mt CO2 eq), whereas AD outperformed the others in avoiding costs of fertilization. According to the estimated potential and the environmental cost-effectiveness of AD, new plants can be established that will recycle manure through bioenergy production, and, subsequently, the digestate can be applied as organic fertilizer, closing the nutrients cycle.
Fast Spectrophotometric Method as Alternative for CuO Oxidation to Assess Lignin in Soils with Different Tree Cover
Authors: Danise Tiziana , Innangi Michele , Curcio Elena , Fioretto Antonietta , Guggenberger Georg
Published: November 2020

Given the ongoing climate change, estimating the amount of less degradable plant compounds that can be stored in the soil, such as lignin, is a topic of primary importance. There are few methods applicable to soils for the determination of lignin, such as the copper oxide (CuO) oxidation method (CuOL). Acetyl bromide spectrophotometric lignin (ABSL) could be a valid alternative providing information that is less detailed compared to CuOL, but it offers data on the bulk amount of lignin and may offer a valid, fast, and cheap alternative to the CuO method. The aim of this work was to compare ABSL with the CuO method on several soils receiving plant residues from different trees. Mineral soil samples from 0 to 10 cm depth were obtained from a former agricultural site in northern Italy (Brusciana, Tuscany), where different tree plantations were established 22 years ago. The plantations were white poplar and common walnut, which were also intercropped with other species such as hazelnut, Italian alder, and autumn olive. Soil samples under these plantations were also compared to soil under an adjacent agricultural field. In general, the amount of lignin in the afforested stands was approximately double than in the agricultural field as determined by either method. The two methods returned a largely different scale of values due to their different mechanisms of action. The acid-to-aldehyde ratio of syringyl structural units highlights that forest plantation provides a plant input material that is more slowly oxidatively degraded compared to arable soil. A linear mixed model proved that ABSL performed well in relation to CuOL, especially when considering the random variation in the model given by the plantation field design. In conclusion, ABSL can be considered a valid proxy of soil C pool derived from structural plant component, although further analyses are needed.
Differences in Forest Use Strategies for Cash Income between Households Living outside and inside Selectively Logged Production Forests in Myanmar
Authors: Saung Thein , Mizoue Nobuya , Ota Tetsuji , Kajisa Tsuyoshi
Published: November 2020

In many tropical regions, rural households often depend on forests for cash income, but there is still little knowledge on how forest use strategies differ among people living in different locations. This study aimed to detect differences in forest use strategies and forest cash income dependency between households living outside and inside selectively logged production forests, known as Reserved Forests (RFs), in Bago Township, Myanmar. A questionnaire survey was conducted with 146 and 48 households living outside and inside the RFs, respectively. The inside-households (encroachers) had a much higher forest cash income dependency (83%), with charcoal production as the main forest use activity, than the outside-households (32%), with bamboo cutting as the main activity. Higher forest dependency was found for outside-households that had less farmland, better accessibility to forest and more recognition of prohibited access to RFs in forest law. This study revealed evidence of substantial forest use for commercial purposes in RFs by households living both inside and outside the RFs, despite local recognition of the illegality of the use. Implementing community forestry practices for local communities may be a better option to reduce illegal dependence on selectively logged production forests.
Aegean Trees and Timbers: Dendrochronological Survey of the Island of Symi
Authors: Christopoulou Anastasia , Gmińska-Nowak Barbara , Özarslan Yasemin , Ważny Tomasz
Published: November 2020

The current study presents the results of the first dendrochronological survey performed over the East Aegean island of Symi. Research Highlights: Dendrochronological research of the East Aegean region is of paramount importance since dendrochronological data from the region, and especially the islands, are still limited. Background and Objectives: The main aim of the study is to explore the dendrochronological potential of the island, focusing on the dating of historical wood and buildings as well as dendroprovenancing. Materials and Methods: A total of 57 wood samples were collected from historical timber from windmills and architectural elements, including doors and warehouse planks, while 68 cores were collected from the three dominant tree species of the island—Cupressus sempervirens, Pinus brutia, and Quercus ithaburensis subsp. macrolepis—in an attempt to develop local reference chronologies that could be useful in dating historical timber Results: Of the historical timber, at least nine different species have been detected, with conifers representing the majority of the collected material. In total, 56% of the dendroarchaeological samples, belonging to four different species, were dated absolutely. According to cross-dating and dendroprovenancing results, Pinus nigra, Cedrus sp., and Quercus sp. represent timber imported from present-day Turkey while the fir samples collected from the windmills originate from Central Europe. The use of local timber is also highly probable although it could not be confirmed by the reference chronologies developed for the three dominant tree species of the island. Conclusions: The results of the study reveal the dendrochronological potential of the island from both dendroarchaeological and dendroecological perspectives. The finding that most of the wood was imported mainly from Turkey highlights the importance of timber trade with the Turkish mainland during the mid-18th and 19th centuries. Chronologies developed from living trees could be used in future studies for dating historical material while further research would increase our understanding of past timber trade and the island’s history.
LAMP-LFD Based on Isothermal Amplification of Multicopy Gene ORF160b: Applicability for Highly Sensitive Low-Tech Screening of Allergenic Soybean (Glycine max) in Food
Authors: Allgöwer Stefanie M. , Hartmann Chris A. , Lipinski Clarissa , Mahler Vera , Randow Stefanie , Völker Elke , Holzhauser Thomas
Published: November 2020

Soybean (Glycine max) allergy can be life threatening. A lack of causative immunotherapy of soybean allergy makes soybean avoidance indispensable. Detection methods are essential to verify allergen labeling and unintentional allergen cross contact during food manufacture. Here, we aimed at evaluating our previously described primers for loop-mediated isothermal amplification (LAMP) of multicopy gene ORF160b, combined with a lateral flow dipstick (LFD)-like detection, for their performance of soybean detection in complex food matrices. The results were compared with those obtained using quantitative real-time Polymerase Chain Reaction (qPCR) as the current standard of DNA-based allergen detection, and antibody-based commercial lateral flow device (LFD) as the current reference of protein-based rapid allergen detection. LAMP-LFD allowed unequivocal and reproducible detection of 10 mg/kg soybean incurred in three representative matrices (boiled sausage, chocolate, instant tomato soup), while clear visibility of positive test lines of two commercial LFD tests was between 10 and 102 mg/kg and depending on the matrix. Sensitivity of soybean detection in incurred food matrices, commercial retail samples, as well as various processed soybean products was comparable between LAMP-LFD and qPCR. The DNA-based LAMP-LFD proved to be a simple and low-technology soybean detection tool, showing sensitivity and specificity that is comparable or superior to the investigated commercial protein-based LFD.
Regional Characterization Study of Fatty Acids and Tocopherol in Organic Milk as a Tool for Potential Geographical Identification
Authors: Chung Ill-Min , Kim Yun-Ju , Moon Hee-Sung , Kwon Chang , Chi Hee-Youn , Kim Seung-Hyun
Published: November 2020

Reliable geographical identification can protect producers of excellent agroproducts, and also provide reliable purchasing information to satisfy consumers. Therefore, this study aimed to evaluate the regional and monthly variation in fatty acid (FA) and tocopherol (TOC) in organic milk (OM) and develop a geographical discriminant model of OM in Korea. In this study, OM had α-TOC and showed a regional or monthly difference of 3–5%. Moreover, C16:0, C18:1 n9 cis + trans, C18:0, and C14:0 were the predominant FAs in OM, and OM mostly had higher ∑UFA, including nutritionally desirable FAs; but lower ∑SFA among four regions or in April and August (p < 0.001). The model prepared using stepwise discriminant analysis showed a classification accuracy of 100% for original and cross-validated sample sets. Our results have characterized regional and monthly nutritional variations of OM, thereby potentially suggesting the applicability of a reliable Korean geographical identification labeling system using nutrient compositional analysis of OM.
Can a Corn-Derived Biosurfactant Improve Colour Traits of Wine? First Insight on Its Application during Winegrape Skin Maceration versus Oenological Tannins
Authors: Scalzini Giulia , López-Prieto Alejandro , Paissoni Maria A. , Englezos Vasileios , Giacosa Simone , Rolle Luca , Gerbi Vincenzo , Río Segade Susana , Pérez Cid Benita , Moldes Ana B. , Cruz Jose M.
Published: November 2020

In winemaking, oenological tannins are used to preserve wine colour by enhancing the antioxidant activity, taking part in copigmentation, and forming polymeric pigments with anthocyanins. As a novel processing aid, in this study, a biosurfactant extract was evaluated as a solubilizing and stabilizing agent of anthocyanins in red wine. The biosurfactant extract under evaluation was obtained from a fermented residual stream of the corn milling industry named corn steep liquor (CSL). Two red winegrape varieties (Vitis vinifera L. cv. Aglianico and Cabernet sauvignon) were studied for anthocyanin content and profile, and colour traits, during simulated skin maceration for 7 days at 25 °C, as well as polymerization and copigmentation at the end of maceration. A model wine solution was used as a control, which was added either with the CSL biosurfactant or with four different oenological tannins (from grape skin, grape seed, quebracho, and acacia). The results showed that CSL biosurfactant addition improved the colour properties of skin extracts by the formation of more stable compounds mainly through copigmentation interactions. These preliminary results highlighted that the effectiveness of CSL biosurfactant is variety-dependent; however, there is no significant protection of individual anthocyanin compounds as observed for delphinidin and petunidin forms using quebracho tannin.
Physicochemical and Sensorial Evaluation of Meat Analogues Produced from Dry-Fractionated Pea and Oat Proteins
Authors: De Angelis Davide , Kaleda Aleksei , Pasqualone Antonella , Vaikma Helen , Tamm Martti , Tammik Mari-Liis , Squeo Giacomo , Summo Carmine
Published: November 2020

Pea protein dry-fractionated (PDF), pea protein isolated (PIs), soy protein isolated (SIs) and oat protein (OP) were combined in four mixes (PDF_OP, PIs_OP, PDF_PIs_OP, SIs_OP) and extruded to produce meat analogues. The ingredients strongly influenced the process conditions and the use of PDF required higher specific mechanical energy and screw speed to create fibrous texture compared to PIs and SIs. PDF can be conveniently used to produce meat analogues with a protein content of 55 g 100 g−1, which is exploitable in meat-alternatives formulation. PDF-based meat analogues showed lower hardness (13.55–18.33 N) than those produced from PIs and SIs (nearly 27 N), probably due to a more porous structure given by the natural presence of carbohydrates in the dry-fractionated ingredient. PDF_OP and PIs_PDF_OP showed a significantly lower water absorption capacity than PIs OP and SIs_OP, whereas pea-based extrudates showed high oil absorption capacity, which could be convenient to facilitate the inclusion of oil and fat in the final formulation. The sensory evaluation highlighted an intense odor and taste profile of PDF_OP, whereas the extrudates produced by protein isolates had more neutral sensory characteristics. Overall, the use of dry-fractionated protein supports the strategies to efficiently produce clean-labeled and sustainable plant-based meat analogues.
Antilisterial Activity of Bacteriocins Produced by Lactic Bacteria Isolated from Dairy Products
Authors: Niederhäusern Simona de , Camellini Stefania , Sabia Carla , Iseppi Ramona , Bondi Moreno , Messi Patrizia
Published: November 2020

Sixty-nine Lactic Acid Bacteria (LAB) and bifidobacteria were isolated and identified from Italian dairy products (raw milk, cream, butter, soft cheese and yoghurt) to find new antimicrobial compounds to use as food bio-preservatives. All the isolates were preliminarily screened by the deferred antagonism method for bacteriocin production. Afterwards, to evaluate the release of bacteriocin in liquid medium, the Cell-Free Supernatant Fluid (CFSF) of the best producers was tested by agar well diffusion assay. The study allowed the selection of three bacteriocin producing strains (Enterococcus faecium E23, Bifidobacterium thermophilum B23 and Lactobacillus bulgaricus L21), endowed with the strongest and broadest inhibitory capability against the pathogen Listeria monocytogenes. The molecular characteristics and the chemical–physical properties of both producers and the respective bacteriocins were studied and compared. The results showed that E. faecium E23 was the best producer strain and its class IIa bacteriocins, called enterocin E23, exhibited a good spectrum of activity towards L. monocytogenes. Enterocin E23 was stable over a wide range of pH and at low temperatures for at least four months and, for this reason, it can be employed in refrigerated foods for the control of L. monocytogenes, the major concern in dairy products.
An Extremum Principle for Smooth Problems
Authors: Idczak Dariusz , Walczak Stanisław
Published: November 2020

We derive an extremum principle. It can be treated as an intermediate result between the celebrated smooth-convex extremum principle due to Ioffe and Tikhomirov and the Dubovitskii–Milyutin theorem. The proof of this principle is based on a simple generalization of the Fermat’s theorem, the smooth-convex extremum principle and the local implicit function theorem. An integro-differential example illustrating the new principle is presented.
Evolutionary Understanding of Metacaspase Genes in Cultivated and Wild Oryza Species and Its Role in Disease Resistance Mechanism in Rice
Authors: Bansal Ruchi , Rana Nitika , Singh Akshay , Dhiman Pallavi , Mandlik Rushil , Sonah Humira , Deshmukh Rupesh , Sharma Tilak Raj
Published: November 2020

Metacaspases (MCs), a class of cysteine-dependent proteases found in plants, fungi, and protozoa, are predominately involved in programmed cell death processes. In this study, we identified metacaspase genes in cultivated and wild rice species. Characterization of metacaspase genes identified both in cultivated subspecies of Oryza sativa, japonica, and indica and in nine wild rice species was performed. Extensive computational analysis was conducted to understand gene structures, phylogenetic relationships, cis-regulatory elements, expression patterns, and haplotypic variations. Further, the haplotyping study of metacaspase genes was conducted using the whole-genome resequencing data publicly available for 4726 diverse genotype and in-house resequencing data generated for north-east Indian rice lines. Sequence variations observed among wild and cultivated rice species for metacaspase genes were used to understand the duplication and neofunctionalization events. The expression profiles of metacaspase genes were analyzed using RNA-seq transcriptome profiling in rice during different developmental stages and stress conditions. Real-time quantitative PCR analysis of candidate metacaspase genes in rice cultivars Pusa Basmati-1 in response to Magnaporthe oryzae infection indicated a significant role in the disease resistance mechanism. The information provided here will help to understand the evolution of metacaspases and their role under stress conditions in rice.
The Plasticity of Genome Architecture
Authors: Farré Marta , Ruiz-Herrera Aurora
Published: November 2020

Understanding the origin of species and their adaptability to new environments is one of the main questions in biology [...]
Antisense Transcription across Nucleotide Repeat Expansions in Neurodegenerative and Neuromuscular Diseases: Progress and Mysteries
Authors: Castro Ana F. , Loureiro Joana R. , Bessa José , Silveira Isabel
Published: November 2020

Unstable repeat expansions and insertions cause more than 30 neurodegenerative and neuromuscular diseases. Remarkably, bidirectional transcription of repeat expansions has been identified in at least 14 of these diseases. More remarkably, a growing number of studies has been showing that both sense and antisense repeat RNAs are able to dysregulate important cellular pathways, contributing together to the observed clinical phenotype. Notably, antisense repeat RNAs from spinocerebellar ataxia type 7, myotonic dystrophy type 1, Huntington’s disease and frontotemporal dementia/amyotrophic lateral sclerosis associated genes have been implicated in transcriptional regulation of sense gene expression, acting either at a transcriptional or posttranscriptional level. The recent evidence that antisense repeat RNAs could modulate gene expression broadens our understanding of the pathogenic pathways and adds more complexity to the development of therapeutic strategies for these disorders. In this review, we cover the amazing progress made in the understanding of the pathogenic mechanisms associated with repeat expansion neurodegenerative and neuromuscular diseases with a focus on the impact of antisense repeat transcription in the development of efficient therapies.
Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells
Authors: Yokosawa Takumi , Sato Aomi , Wakasugi Keisuke
Published: November 2020

The novel high-affinity tryptophan (Trp)-selective transport system is present at elevated levels in human interferon-γ (IFN-γ)-treated and indoleamine 2,3-dioxygenase 1 (IDO1)-expressing cells. High-affinity Trp uptake into cells results in extracellular Trp depletion and immune suppression. We have previously shown that both IDO1 and tryptophanyl-tRNA synthetase (TrpRS), whose expression levels are increased by IFN-γ, have a crucial function in high-affinity Trp uptake into human cells. Here, we aimed to elucidate the relationship between TrpRS and IDO1 in high-affinity Trp uptake. We demonstrated that overexpression of IDO1 in HeLa cells drastically enhances high-affinity Trp uptake upon addition of purified TrpRS protein to uptake assay buffer. We also clarified that high-affinity Trp uptake by Trp-starved cells is significantly enhanced by the addition of TrpRS protein to the assay buffer. Moreover, we showed that high-affinity Trp uptake is also markedly elevated by the addition of TrpRS protein to the assay buffer of cells overexpressing another Trp-metabolizing enzyme, tryptophan 2,3-dioxygenase (TDO2). Taken together, we conclude that Trp deficiency is crucial for high-affinity Trp uptake mediated by extracellular TrpRS.
A Scoping Review of the Risk Factors Associated with Anaemia among Children Under Five Years in Sub-Saharan African Countries
Authors: Obasohan Phillips Edomwonyi , Walters Stephen J. , Jacques Richard , Khatab Khaled
Published: November 2020

Background/Purpose: Globally, anaemia is a severe public health condition affecting over 24% of the world’s population. Children under five years old and pregnant women are the most vulnerable to this disease. This scoping review aimed to evaluate studies that used classical statistical regression methods on nationally representative health survey data to identify the individual socioeconomic, demographic and contextual risk factors associated with developing anaemia among children under five years of age in sub-Saharan Africa (SSA). Methods/Design: The reporting pattern followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) guidelines. The following databases were searched: MEDLINE, EMBASE (OVID platform), Web of Science, PUBMED, Cumulative Index to Nursing and Allied Health Literature (CINAHL), PsycINFO, Scopus, Cochrane library, African Journal of online (AJOL), Google Scholar and Measure DHS. Results: The review identified 20 relevant studies and the risk factors for anaemia were classified as child-related, parental/household-related and community- or area-related factors. The risk factors for anaemia identified included age, birth order, sex, comorbidities (such as fever, diarrhoea and acute respiratory infection), malnutrition or stunting, maternal education, maternal age, mother’s anaemia status, household wealth and place of residence. Conclusion: The outcome of this review is of significant value for health policy and planners to enable them to make informed decision that will correct any imbalances in anaemia across socioeconomic, demographic and contextual characteristics, with the view of making efficient distributions of health interventions.
Role of Metabolism in Bone Development and Homeostasis
Authors: Suzuki Akiko , Minamide Mina , Iwaya Chihiro , Ogata Kenichi , Iwata Junichi
Published: November 2020

Carbohydrates, fats, and proteins are the underlying energy sources for animals and are catabolized through specific biochemical cascades involving numerous enzymes. The catabolites and metabolites in these metabolic pathways are crucial for many cellular functions; therefore, an imbalance and/or dysregulation of these pathways causes cellular dysfunction, resulting in various metabolic diseases. Bone, a highly mineralized organ that serves as a skeleton of the body, undergoes continuous active turnover, which is required for the maintenance of healthy bony components through the deposition and resorption of bone matrix and minerals. This highly coordinated event is regulated throughout life by bone cells such as osteoblasts, osteoclasts, and osteocytes, and requires synchronized activities from different metabolic pathways. Here, we aim to provide a comprehensive review of the cellular metabolism involved in bone development and homeostasis, as revealed by mouse genetic studies.
Neural Stem Cell-Derived Exosomes Revert HFD-Dependent Memory Impairment via CREB-BDNF Signalling
Authors: Spinelli Matteo , Natale Francesca , Rinaudo Marco , Leone Lucia , Mezzogori Daniele , Fusco Salvatore , Grassi Claudio
Published: November 2020

Overnutrition and metabolic disorders impair cognitive functions through molecular mechanisms still poorly understood. In mice fed with a high fat diet (HFD) we analysed the expression of synaptic plasticity-related genes and the activation of cAMP response element-binding protein (CREB)-brain-derived neurotrophic factor (BDNF)-tropomyosin receptor kinase B (TrkB) signalling. We found that a HFD inhibited both CREB phosphorylation and the expression of a set of CREB target genes in the hippocampus. The intranasal administration of neural stem cell (NSC)-derived exosomes (exo-NSC) epigenetically restored the transcription of Bdnf, nNOS, Sirt1, Egr3, and RelA genes by inducing the recruitment of CREB on their regulatory sequences. Finally, exo-NSC administration rescued both BDNF signalling and memory in HFD mice. Collectively, our findings highlight novel mechanisms underlying HFD-related memory impairment and provide evidence of the potential therapeutic effect of exo-NSC against metabolic disease-related cognitive decline.
DNA Double-Strand Breaks Are a Critical Regulator of Fear Memory Reconsolidation
Authors: Navabpour Shaghayegh , Rogers Jessie , McFadden Taylor , Jarome Timothy J.
Published: November 2020

Numerous studies have shown that following retrieval, a previously consolidated memory requires increased transcriptional regulation in order to be reconsolidated. Previously, it was reported that histone H3 lysine-4 trimethylation (H3K4me3), a marker of active transcription, is increased in the hippocampus after the retrieval of contextual fear memory. However, it is currently unknown how this epigenetic mark is regulated during the reconsolidation process. Furthermore, though recent evidence suggests that neuronal activity triggers DNA double-strand breaks (DSBs) in some early-response genes, it is currently unknown if DSBs contribute to the reconsolidation of a memory following retrieval. Here, using chromatin immunoprecipitation (ChIP) analyses, we report a significant overlap between DSBs and H3K4me3 in area CA1 of the hippocampus during the reconsolidation process. We found an increase in phosphorylation of histone H2A.X at serine 139 (H2A.XpS139), a marker of DSB, in the Npas4, but not c-fos, promoter region 5 min after retrieval, which correlated with increased H3K4me3 levels, suggesting that the two epigenetic marks may work in concert during the reconsolidation process. Consistent with this, in vivo siRNA-mediated knockdown of topoisomerase II β, the enzyme responsible for DSB, prior to retrieval, reduced Npas4 promoter-specific H2A.XpS139 and H3K4me3 levels and impaired long-term memory, indicating an indispensable role of DSBs in the memory reconsolidation process. Collectively, our data propose a novel mechanism for memory reconsolidation through increases in epigenetic-mediated transcriptional control via DNA double-strand breaks.
Eosinophil microRNAs Play a Regulatory Role in Allergic Diseases Included in the Atopic March
Authors: Bélanger Émile , Madore Anne-Marie , Boucher-Lafleur Anne-Marie , Simon Marie-Michelle , Kwan Tony , Pastinen Tomi , Laprise Catherine
Published: November 2020

(1) Background: The atopic march is defined by the increased prevalence of allergic diseases after atopic dermatitis onset. In fact, atopic dermatitis is believed to play an important role in allergen sensitization via the damaged skin barrier, leading to allergic diseases such as allergic asthma and allergic rhinitis. The eosinophil, a pro-inflammatory cell that contributes to epithelial damage, is one of the various cells recruited in the inflammatory reactions characterizing these diseases. Few studies were conducted on the transcriptome of this cell type and even less on their specific microRNA (miRNA) profile, which could modulate pathogenesis of allergic diseases and clinical manifestations post-transcriptionally. Actually, their implication in allergic diseases is not fully understood, but they are believed to play a role in inflammation-related patterns and epithelial cell proliferation. (2) Methods: Next-generation sequencing was performed on RNA samples from eosinophils of individuals with atopic dermatitis, atopy, allergic rhinitis and asthma to obtain differential counts of primary miRNA (pri-miRNA); these were also analyzed for asthma-related phenotypes such as forced expiratory volume in one second (FEV1), immunoglobulin E (IgE) and provocative concentration of methacholine inducing a 20% fall in forced expiratory volume in 1 s (PC20) levels, as well as FEV1 to forced vital capacity (FEV1/FVC) ratio. (3) Results: Eighteen miRNAs from eosinophils were identified to be significantly different between affected individuals and unaffected ones. Based on counts from these miRNAs, individuals were then clustered into groups using Ward’s method on Euclidian distances. Groups were found to be explained by asthma diagnosis, familial history of respiratory diseases and allergic rhinitis as well as neutrophil counts. (4) Conclusions: The 18 differential miRNA counts for the studying phenotypes allow a better understanding of the epigenetic mechanisms underlying the development of the allergic diseases included in the atopic march.
Pan-Sigma Receptor Modulator RC-106 Induces Terminal Unfolded Protein Response In In Vitro Pancreatic Cancer Model
Authors: Cortesi Michela , Zamagni Alice , Pignatta Sara , Zanoni Michele , Arienti Chiara , Rossi Daniela , Collina Simona , Tesei Anna
Published: November 2020

Pancreatic cancer (PC) remains one of the most lethal cancers worldwide. Sigma receptors (SRs) have been proposed as cancer therapeutic targets. Their main localization suggests they play a potential role in ER stress and in the triggering of the unfolded protein response (UPR). Here, we investigated the mechanisms of action of RC-106, a novel pan-SR modulator, to characterize therapeutically exploitable role of SRs in tumors. Two PC cell lines were used in all the experiments. Terminal UPR activation was evaluated by quantifying BiP, ATF4 and CHOP by Real-Time qRT-PCR, Western Blot, immunofluorescence and confocal microscopy. Cell death was studied by flow cytometry. Post-transcriptional gene silencing was performed to study the interactions between SRs and UPR key proteins. RC-106 activated ER stress sensors in a dose- and time-dependent manner. It also induced ROS production accordingly with ATF4 upregulation at the same time reducing cell viability of both cell lines tested. Moreover, RC-106 exerted its effect through the induction of the terminal UPR, as shown by the activation of some of the main transducers of this pathway. Post-transcriptional silencing studies confirmed the connection between SRs and these key proteins. Overall, our data highlighted a key role of SRs in the activation of the terminal UPR pathway, thus indicating pan-SR ligands as candidates for targeting the UPR in pancreatic cancer.