bluuebunny/crossref_metadata_2025 huggingface dataset:
Security and Privacy Analysis of Vinoth et al.’s Authenticated Key Agreement Scheme for Industrial IoT
Authors: Sun Da-Zhi
Published: October 2021

Vinoth et al. proposed an authenticated key agreement scheme for industrial IoT (Internet of Things) applications. Vinoth et al.’s scheme aimed to protect the remote sensing data of industrial IoT devices under hostile environments. The scheme is interesting because the authorized user is allowed simultaneously to access the multiple IoT sensing devices. Therefore, we carefully analyzed the security and privacy implications of Vinoth et al.’s scheme. Our findings are summarized as follows. One, Vinoth et al.’s scheme failed to defeat user impersonation attacks. Second, Vinoth et al.’s scheme did not prevent IoT sensing device impersonation attacks. Third, Vinoth et al.’s scheme suffered from replay attacks. Fourth, Vinoth et al.’s scheme was vulnerable to desynchronization attacks. Fifth, Vinoth et al.’s scheme could not maintain user privacy. As a case study, our analysis results enlighten researchers and engineers on the design of robust and efficient authenticated key agreement schemes for IoT applications.
Cortical Activation in Mental Rotation and the Role of the Corpus Callosum: Observations in Healthy Subjects and Split-Brain Patients
Authors: Pierpaoli Chiara , Ghoushi Mojgan , Foschi Nicoletta , Lattanzi Simona , Fabri Mara , Polonara Gabriele
Published: October 2021

The mental rotation (MR) is an abstract mental operation thanks to which a person imagines rotating an object or a body part to place it in an other position. The ability to perform MR was belived to belong to the right hemisphere for objects, and to the left for one’s ownbody images. Mental rotation is considered to be basic for imitation with the anatomical perspective, which in turn is needed for social interactions and learning. Altered imitative performances have been reported in patients with resections or microstructure alterations of the corpus callosum (CC). These patients also display a reduced MR ability compared to control subjects, as shown in a recent behavioral study. The difference was statistically significant, leading us to hypothesize a role of the CC to integrate the two hemispheres’ asymmetric functions. The present study was designed to detect, by means of a functional MRI, the cortical activation evoked during an MR task in healthy control subjects and callosotomized patients. The results suggest that performing MR requires activation of opercular cortex and inferior parietal lobule in either hemispheres, and likely the integrity of the CC, thus confirming that the main brain commissure is involved in cognitive functions.
A Study of Improving Running Safety of a Railway Wagon with an Independently Rotating Wheel’s Flange
Authors: Mikhailov Evgeny , Semenov Stanislav , Shvornikova Hanna , Gerlici Juraj , Kovtanets Maxim , Dižo Ján , Blatnický Miroslav , Harušinec Jozef
Published: October 2021

The main objective of this work is to study the possibilities of improving the running safety of a railway wagon with independently rotating wheels by changing their design symmetrically mounted on an axle. The article provides a discussion of the advantages and disadvantages of using the independently rotating wheels in a bogie of railway wagons. Their increasing tendency of derailment is described. The influence of a perspective constructive scheme (PKS) of railway wagon wheels in comparison with a traditional constructive scheme (TKS) on running safety due to the climbing of a wheel flange onto a rail is studied. This work introduces a conceptual proposition of a technical solution to railway wheel design as well as containing the results of both analytical calculations as well as the results of multibody simulations. A PKS wheel design for a railway wheel is designed that allows independent rotation of its tread surface and of a guiding surface (i.e., of a flange) to each other, which both are arranged symmetrically on a wheelset axle. It brings features of the distribution of friction forces generating in a flange contact when the wheel with a TKS and with PKS move on a rail. It is possible to conclude with the help of the obtained results that the use of wheels with the PKS is advisable for the reduction of the running resistance as well as for increasing the running safety of railway wagons.
An Ulm-Type Inverse-Free Iterative Scheme for Fredholm Integral Equations of Second Kind
Authors: Gutiérrez José M. , Hernández-Verón Miguel Á.
Published: October 2021

In this paper, we present an iterative method based on the well-known Ulm’s method to numerically solve Fredholm integral equations of the second kind. We support our strategy in the symmetry between two well-known problems in Numerical Analysis: the solution of linear integral equations and the approximation of inverse operators. In this way, we obtain a two-folded algorithm that allows us to approximate, with quadratic order of convergence, the solution of the integral equation as well as the inverses at the solution of the derivative of the operator related to the problem. We have studied the semilocal convergence of the method and we have obtained the expression of the method in a particular case, given by some adequate initial choices. The theoretical results are illustrated with two applications to integral equations, given by symmetric non-separable kernels.
Effects of Deoxynivalenol and Fumonisins on Broiler Gut Cytoprotective Capacity
Authors: Paraskeuas Vasileios , Griela Eirini , Bouziotis Dimitrios , Fegeros Konstantinos , Antonissen Gunther , Mountzouris Konstantinos C.
Published: October 2021

Mycotoxins are a crucial problem for poultry production worldwide. Two of the most frequently found mycotoxins in feedstuffs are deoxynivalenol (DON) and fumonisins (FUM) which adversely affect gut health and poultry performance. The current knowledge on DON and FUM effects on broiler responses relevant for gut detoxification, antioxidant capacity, and health is still unclear. The aim of this study was to assess a range of selected molecular intestinal biomarkers for their responsiveness to the maximum allowable European Union dietary levels for DON (5 mg/kg) and FUM (20 mg/kg) in broilers. For the experimental purpose, a challenge diet was formulated, and biomarkers relevant for detoxification, antioxidant response, stress, inflammation, and integrity were profiled across the broiler intestine. The results reveal that DON significantly (p < 0.05) induced aryl hydrocarbon receptor (AhR) and cytochrome P450 enzyme (CYP) expression mainly at the duodenum. Moreover, DON and FUM had specific significant (p < 0.05) effects on the antioxidant response, stress, inflammation, and integrity depending on the intestinal segment. Consequently, broiler molecular responses to DON and FUM assessed via a powerful palette of biomarkers were shown to be mycotoxin and intestinal site specific. The study findings could be highly relevant for assessing various dietary bioactive components for protection against mycotoxins.
Occurrence and Characterization of Penicillium Species Isolated from Post-Harvest Apples in Lebanon
Authors: Habib Wassim , Masiello Mario , Chahine-Tsouvalakis Hala , Al Moussawi Zahraa , Saab Carine , Tawk Salwa Tohmé , Piemontese Luca , Solfrizzo Michele , Logrieco Antonio Francesco , Moretti Antonio , Susca Antonia
Published: October 2021

The apple is one of the most important fruit tree crops in the Mediterranean region. Lebanon, in particular, is among the top apple producer countries in the Middle East; however, recently, several types of damage, particularly rot symptoms, have been detected on fruits in cold storage. This study aims to identify the causal agents of apple decay in Lebanese post-harvest facilities and characterize a set of 39 representative strains of the toxigenic fungus Penicillium. The results demonstrated that blue mould was the most frequent fungal disease associated with apples showing symptoms of decay after 3–4 months of storage at 0 °C, with an average frequency of 76.5% and 80.6% on cv. Red and cv. Golden Delicious apples, respectively. The morphological identification and phylogenetic analysis of benA gene showed that most Penicillium strains (87.2%) belong to P. expansum species whereas the remaining strains (12.8%) belong to P. solitum. Furthermore, 67.7% of P. expansum strains produced patulin when grown on apple puree for 14 days at 25 °C with values ranging from 10.7 mg kg−1 to 125.9 mg kg−1, whereas all P. solitum did not produce the mycotoxin. This study highlights the presence of Penicillium spp. and their related mycotoxin risk during apple storage and calls for the implementation of proper measures to decrease the risk of mycotoxin contamination of apple fruit products.
The Protective Effect of Heme Oxygenase-1 on Liver Injury Caused by DON-Induced Oxidative Stress and Cytotoxicity
Authors: Meng Zitong , Wang Liangliang , Liao Yuxiao , Peng Zhao , Li Dan , Zhou Xiaolei , Liu Shuang , Li Yanmei , Nüssler Andreas K. , Liu Liegang , Hao Liping , Yang Wei
Published: October 2021

Deoxynivalenol (DON) is a kind of Fusarium toxin that can cause a variety of toxic effects. Oxidative stress and DNA damage play a critical role in the toxicity of DON. However, previous studies focused more on acute toxicity in vivo/vitro models and lacked subchronic toxicity study in vivo. The potentially harmful effect of DON given at doses comparable to the daily human consumption in target organs, especially the liver, which is the main detoxification organ of DON, is also still not fully understood. Otherwise, Heme Oxygenase-1 (HO-1) has also reduced cell damage under the DON condition according to our previous study. Therefore, we used a rodent model that mimicked daily human exposure to DON and further explored its mechanism of toxic effects on liver tissue and Hepa 1–6 cell line. We also used adeno-associated virus (AAV)-modified HO-1 expressing by tail vein injection and constructed lentivirus-Hepa 1–6 cell line for mimicking HO-1 protective ability under the DON condition. The main results showed that both 30 d and 90 d exposures of DON could cause low-grade inflammatory infiltration around hepatic centrilobular veins. The reactive oxygen species (ROS) and 8-hydroxy-2 deoxyguanosine (8-OHdG) increased during DON exposure, indicating oxidation stress and DNA damage. Significantly, AAV-mediated liver-specific overexpression of HO-1 reduced DON-induced liver damage and indirectly protected the abilities of antioxidant enzyme/DNA damage repair system, while AAV-mediated silence of HO-1 produced the opposite effect. In addition, we found that overexpression of HO-1 could enhance autophagy and combined it with an antioxidant enzyme/DNA damage repair system to inhibit DON-induced hepatocyte damage. Altogether, these data suggest that HO-1 reduces the oxidative stress and DNA damage caused by DON sub-chronic exposure through maintaining DNA repair, antioxidant activity, as well as autophagy.
Equation of State and Composition of Proto-Neutron Stars and Merger Remnants with Hyperons
Authors: Sedrakian Armen , Harutyunyan Arus
Published: October 2021

Finite-temperature equation of state (EoS) and the composition of dense nuclear and hypernuclear matter under conditions characteristic of neutron star binary merger remnants and supernovas are discussed. We consider both neutrino free-streaming and trapped regimes which are separated by a temperature of a few MeV. The formalism is based on covariant density functional (CDF) theory for the full baryon octet with density-dependent couplings, suitably adjusted in the hypernuclear sector. The softening of the EoS with the introduction of the hyperons is quantified under various conditions of lepton fractions and temperatures. We find that Λ, Ξ−, and Ξ0 hyperons appear in the given order with a sharp density increase at zero temperature at the threshold being replaced by an extended increment over a wide density range at high temperatures. The Λ hyperon survives in the deep subnuclear regime. The triplet of Σs is suppressed in cold hypernuclear matter up to around seven times the nuclear saturation density, but appears in significant fractions at higher temperatures, T≥20 MeV, in both supernova and merger remnant matter. We point out that a special isospin degeneracy point exists where the baryon abundances within each of the three isospin multiplets are equal to each other as a result of (approximate) isospin symmetry. At that point, the charge chemical potential of the system vanishes. We find that under the merger remnant conditions, the fractions of electron and μ-on neutrinos are close and are about 1%, whereas in the supernova case, we only find a significant fraction (∼10%) of electron neutrinos, given that in this case, the μ-on lepton number is zero.
Comparison of Eight Commercially Available Faecal Point-of-Care Tests for Detection of Canine Parvovirus Antigen
Authors: Walter-Weingärtner Julia , Bergmann Michèle , Weber Karin , Truyen Uwe , Muresan Cosmin , Hartmann Katrin
Published: October 2021

A real-time polymerase chain reaction (qPCR) is considered the gold standard for the laboratory diagnosis of canine parvovirus (CPV) infection but can only be performed in specialized laboratories. Several point-of-care tests (POCT), detecting CPV antigens in faeces within minutes, are commercially available. The aim of this study was to evaluate eight POCT in comparison with qPCR. Faecal samples of 150 dogs from three groups (H: 50 client-owned, healthy dogs, not vaccinated within the last four weeks; S: 50 shelter dogs, healthy, not vaccinated within the last four weeks; p = 50 dogs with clinical signs of CPV infection) were tested with eight POCT and qPCR. Practicability, sensitivity, specificity, positive (PPV) and negative predictive values (NPV), as well as overall accuracy were determined. To assess the differences between and agreement among POCT, McNemar’s test and Cohen’s Kappa statistic were performed. Specificity and PPV were 100.0% in all POCT. Sensitivity varied from 22.9–34.3% overall and from 32.7–49.0% in group P. VetexpertRapidTestCPVAg® had the highest sensitivity (34.3% overall, 49.0% group P) and differed significantly from the 3 POCT with the lowest sensitivities (Fassisi®Parvo (27.7% overall, 36.7% group P), Primagnost®ParvoH+K (24.3% overall, 34.7% group P), FASTest®PARVOCard (22.9% overall, 32.7% group P)). The agreement among all POCT was at least substantial (kappa >0.80). A positive POCT result confirmed the infection with CPV in unvaccinated dogs, whereas a negative POCT result did not definitely exclude CPV infection due to the low sensitivity of all POCT.
Transmission of Grapevine leafroll-associated virus-1 (Ampelovirus) and Grapevine virus A (Vitivirus) by the Cottony Grape Scale, Pulvinaria vitis (Hemiptera: Coccidae)
Authors: Hommay Gérard , Alliaume Antoine , Reinbold Catherine , Herrbach Etienne
Published: October 2021

The cottony grape scale Pulvinaria vitis is a scale insect colonizing grapevine; however, its capacity as a vector of grapevine viruses is poorly known in comparison to other scale species that are vectors of viral species in the genera Ampelovirus and Vitivirus. The ability of P. vitis to transmit the ampeloviruses Grapevine leafroll-associated viruses [GLRaV]−1, −3, and −4, and the vitivirus Grapevine virus A (GVA), to healthy vine cuttings was assessed. The scale insects used originated from commercial vine plots located in Alsace, Eastern France. When nymphs sampled from leafroll-infected vineyard plants were transferred onto healthy cuttings, only one event of transmission was obtained. However, when laboratory-reared, non-viruliferous nymphs were allowed to acquire viruses under controlled conditions, both first and second instar nymphs derived from two vineyards were able to transmit GLRaV−1 and GVA. This is the first report of GLRaV−1 and GVA transmission from grapevine to grapevine by this species.
Molecular Characterization and Taxonomic Assignment of Three Phage Isolates from a Collection Infecting Pseudomonas syringae pv. actinidiae and P. syringae pv. phaseolicola from Northern Italy
Authors: Martino Gabriele , Holtappels Dominique , Vallino Marta , Chiapello Marco , Turina Massimo , Lavigne Rob , Wagemans Jeroen , Ciuffo Marina
Published: October 2021

Bacterial kiwifruit vine disease (Pseudomonas syringae pv. actinidiae, Psa) and halo blight of bean (P. syringae pv. phaseolicola, Pph) are routinely treated with copper, leading to environmental pollution and bacterial copper resistance. An alternative sustainable control method could be based on bacteriophages, as phage biocontrol offers high specificity and does not result in the spread of toxic residues into the environment or the food chain. In this research, specific phages suitable for phage-based biocontrol strategies effective against Psa and Pph were isolated and characterized. In total, sixteen lytic Pph phage isolates and seven lytic Psa phage isolates were isolated from soil in Piedmont and Veneto in northern Italy. Genome characterization of fifteen selected phages revealed that the isolated Pph phages were highly similar and could be considered as isolates of a novel species, whereas the isolated Psa phages grouped into four distinct clades, two of which represent putative novel species. No lysogeny-, virulence- or toxin-related genes were found in four phages, making them suitable for potential biocontrol purposes. A partial biological characterization including a host range analysis was performed on a representative subset of these isolates. This analysis was a prerequisite to assess their efficacy in greenhouse and in field trials, using different delivery strategies.
Effect of Ivermectin and Atorvastatin on Nuclear Localization of Importin Alpha and Drug Target Expression Profiling in Host Cells from Nasopharyngeal Swabs of SARS-CoV-2- Positive Patients
Authors: Segatori Valeria Inés , Garona Juan , Caligiuri Lorena Grisel , Bizzotto Juan , Lavignolle Rosario , Toro Ayelén , Sanchis Pablo , Spitzer Eduardo , Krolewiecki Alejandro , Gueron Geraldine , Alonso Daniel Fernando
Published: October 2021

Nuclear transport and vesicle trafficking are key cellular functions involved in the pathogenesis of RNA viruses. Among other pleiotropic effects on virus-infected host cells, ivermectin (IVM) inhibits nuclear transport mechanisms mediated by importins and atorvastatin (ATV) affects actin cytoskeleton-dependent trafficking controlled by Rho GTPases signaling. In this work, we first analyzed the response to infection in nasopharyngeal swabs from SARS-CoV-2-positive and -negative patients by assessing the gene expression of the respective host cell drug targets importins and Rho GTPases. COVID-19 patients showed alterations in KPNA3, KPNA5, KPNA7, KPNB1, RHOA, and CDC42 expression compared with non-COVID-19 patients. An in vitro model of infection with Poly(I:C), a synthetic analog of viral double-stranded RNA, triggered NF-κB activation, an effect that was halted by IVM and ATV treatment. Importin and Rho GTPases gene expression was also impaired by these drugs. Furthermore, through confocal microscopy, we analyzed the effects of IVM and ATV on nuclear to cytoplasmic importin α distribution, alone or in combination. Results showed a significant inhibition of importin α nuclear accumulation under IVM and ATV treatments. These findings confirm transcriptional alterations in importins and Rho GTPases upon SARS-CoV-2 infection and point to IVM and ATV as valid drugs to impair nuclear localization of importin α when used at clinically-relevant concentrations.
Towards a Precision Medicine Approach and In Situ Vaccination against Prostate Cancer by PSMA-Retargeted oHSV
Authors: Vannini Andrea , Parenti Federico , Bressanin Daniela , Barboni Catia , Zaghini Anna , Campadelli-Fiume Gabriella , Gianni Tatiana
Published: October 2021

Prostate specific membrane antigen (PSMA) is a specific high frequency cell surface marker of prostate cancers. Theranostic approaches targeting PSMA show no major adverse effects and rule out off-tumor toxicity. A PSMA-retargeted oHSV (R-405) was generated which both infected and was cytotoxic exclusively for PSMA-positive cells, including human prostate cancer LNCaP and 22Rv1 cells, and spared PSMA-negative cells. R-405 in vivo efficacy against LLC1-PSMA and Renca-PSMA tumors consisted of inhibiting primary tumor growth, establishing long-term T immune response, immune heating of the microenvironment, de-repression of the anti-tumor immune phenotype, and sensitization to checkpoint blockade. The in situ vaccination protected from distant challenge tumors, both PSMA-positive and PSMA-negative, implying that it was addressed also to LLC1 tumor antigens. PSMA-retargeted oHSVs are a precision medicine tool worth being additionally investigated in the immunotherapeutic and in situ vaccination landscape against prostate cancers.
Evolutionary Dynamics of H5 Highly Pathogenic Avian Influenza Viruses (Clade 2.3.4.4B) Circulating in Bulgaria in 2019–2021
Authors: Zecchin Bianca , Goujgoulova Gabriela , Monne Isabella , Salviato Annalisa , Schivo Alessia , Slavcheva Iskra , Pastori Ambra , Brown Ian H. , Lewis Nicola S. , Terregino Calogero , Fusaro Alice
Published: October 2021

The first detection of a Highly Pathogenic Avian Influenza (HPAI) H5N8 virus in Bulgaria dates back to December 2016. Since then, many outbreaks caused by HPAI H5 viruses from clade 2.3.4.4B have been reported in both domestic and wild birds in different regions of the country. In this study, we characterized the complete genome of sixteen H5 viruses collected in Bulgaria between 2019 and 2021. Phylogenetic analyses revealed a persistent circulation of the H5N8 strain for four consecutive years (December 2016–June 2020) and the emergence in 2020 of a novel reassortant H5N2 subtype, likely in a duck farm. Estimation of the time to the most recent common ancestor indicates that this reassortment event may have occurred between May 2019 and January 2020. At the beginning of 2021, Bulgaria experienced a new virus introduction in the poultry sector, namely a HPAI H5N8 that had been circulating in Europe since October 2020. The periodical identification in domestic birds of H5 viruses related to the 2016 epidemic as well as a reassortant strain might indicate undetected circulation of the virus in resident wild birds or in the poultry sector. To avoid the concealed circulation and evolution of viruses, and the risk of emergence of strains with pandemic potential, the implementation of control measures is of utmost importance, particularly in duck farms where birds display no clinical signs.
Nonhuman Primate Models of Zika Virus Infection and Disease during Pregnancy
Authors: Haese Nicole N. , Roberts Victoria H. J. , Chen Athena , Streblow Daniel N. , Morgan Terry K. , Hirsch Alec J.
Published: October 2021

Since the explosive outbreak of Zika virus in Brazil and South/Central America in 2015–2016, the frequency of infections has subsided, but Zika virus remains present in this region as well as other tropical and sub-tropical areas of the globe. The most alarming aspect of Zika virus infection is its association with severe birth defects when infection occurs in pregnant women. Understanding the mechanism of Zika virus pathogenesis, which comprises features unique to Zika virus as well as shared with other teratogenic pathogens, is key to future prophylactic or therapeutic interventions. Nonhuman primate-based research has played a significant role in advancing our knowledge of Zika virus pathogenesis, especially with regard to fetal infection. This review summarizes what we have learned from these models and potential future research directions.
Viral Shedding among Re-Positive Severe Acute Respiratory Syndrome Coronavirus-2 Positive Individuals in Republic of Korea
Authors: Kim Jeong-Min , Ryu Boyeong , Choe Young June , Jo Hye-Jun , Lee Hyeokjin , Kim Heui Man , Lee Nam-Joo , Rhee Jee Eun , Chung Yoon-Seok , Han Myung-Guk , Kim Eun-Jin , Park Youngjoon , Gwack Jin , Jin Yeowon , Song Jeongsuk , Seo Seunghee , Gill Byoungchul , Kim Hyunyeong , Park Yeeun , Yoo Cheon Kwon , Jeong Eun Kyeong
Published: October 2021

This study investigated the infectivity of severe acute respiratory syndrome (SARS-CoV-2) in individuals who re-tested positive for SARS-CoV-2 RNA after recovering from their primary illness. We investigated 295 individuals with re-positive SARS-CoV-2 polymerase chain reaction (PCR) test results and 836 of their close contacts. We attempted virus isolation in individuals with re-positive SARS-CoV-2 PCR test results using cell culture and confirmed the presence of neutralizing antibodies using serological tests. Viral culture was negative in all 108 individuals with re-positive SARS-CoV-2 PCR test results in whom viral culture was performed. Three new cases of SARS-CoV-2 infection were identified among household contacts using PCR. Two of the three new cases had had contact with the index patient during their primary illness, and all three had antibody evidence of past infection. Thus, there was no laboratory evidence of viral shedding and no epidemiological evidence of transmission among individuals with re-positive SARS-CoV-2 PCR test results.
The Potential Role of COVID-19 in the Pathogenesis of Multiple Sclerosis—A Preliminary Report
Authors: Satheesh Noothan , Salloum-Asfar Salam , Abdulla Sara
Published: October 2021

Coronavirus 2019 (COVID-19) is an infectious respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that mainly affects the lungs. COVID-19 symptoms include the presence of fevers, dry coughs, fatigue, sore throat, headaches, diarrhea, and a loss of taste or smell. However, it is understood that SARS-CoV-2 is neurotoxic and neuro-invasive and could enter the central nervous system (CNS) via the hematogenous route or via the peripheral nerve route and causes encephalitis, encephalopathy, and acute disseminated encephalomyelitis (ADEM) in COVID-19 patients. This review discusses the possibility of SARS-CoV-2-mediated Multiple Sclerosis (MS) development in the future, comparable to the surge in Parkinson’s disease cases following the Spanish Flu in 1918. Moreover, the SARS-CoV-2 infection is associated with a cytokine storm. This review highlights the impact of these modulated cytokines on glial cell interactions within the CNS and their role in potentially prompting MS development as a secondary disease by SARS-CoV-2. SARS-CoV-2 is neurotropic and could interfere with various functions of neurons leading to MS development. The influence of neuroinflammation, microglia phagocytotic capabilities, as well as hypoxia-mediated mitochondrial dysfunction and neurodegeneration, are mechanisms that may ultimately trigger MS development.
Immune Response in Mice Immunized with Chimeric H1 Antigens
Authors: Kotey Erasmus Nikoi , Ampofo William Kwabena , Daines Rebecca , Sadeyen Jean-Remy , Iqbal Munir , Quaye Osbourne
Published: October 2021

Identification of a universal influenza vaccine candidate has remained a global challenge for both humans and animals. This study describes an approach that uses consensus sequence building to generate chimeric HAs (cHAs): two resultant H1 HA-based chimeras comprising of conserved sequences (within several areas spanning the head and stalk regions) of H1 and H5 or H9 HAs. These cHAs expressed in Drosophila cells (S2) were used to immunize mice. All immunized mice were protected from an infectious H1 virus challenge. Seroconverted mice sera to the H1 cHAs inhibited both the challenge virus and an H5 virus isolate by haemagglutination inhibition (HI) assay. These findings further emphasize that cHAs induce cross-reactive antibodies against conserved areas of both head and stalk regions of the seasonal influenza A (H1N1) pdm09 virus’ HA and holds potential for further development of a universal influenza vaccine.
Pharmaceutical Industry’s Engagement in the Global Equitable Distribution of COVID-19 Vaccines: Corporate Social Responsibility of EUL Vaccine Developers
Authors: Sung Meekang , Huang Yangmu , Duan Yuqi , Liu Fangjing , Jin Yinzi , Zheng Zhijie
Published: October 2021

(1) Objectives: Inequality in the global distribution of COVID-19 vaccines has brought about great challenges in terms of resolving the pandemic. Although vaccine manufacturers are undoubtedly some of the most influential players, studies on their role in global vaccine distribution have been scarce. This study examined whether the pharmaceutical industry is acting according to the principles of corporate social responsibility (CSR) during the pandemic. (2) Methods: Three categories were used to analyze the CSR of vaccine developers. The first was research and development: effectiveness, funding, and profits were measured. The second was transparency and accountability: the transparency of clinical trials and vaccine contracts was analyzed. The final was vaccine delivery: the status of the provision of vaccines to COVAX and lower-income countries, intellectual property management, manufacturing agreements, and equitable pricing were measured. (3) Results: Vaccine developers have acquired large profits. The vaccine delivery category faces the most challenges. Participation of pharmaceutical companies through COVAX was significantly low, and most vaccine supply agreements were secretive, bilateral deals. It was not clear if companies were maintaining equitable pricing. The evaluation indicated that the companies’ CSR practices have differed during the pandemic. (4) Conclusions: Our study contributes to the methodology of assessing the CSR of vaccine developers. This would help understand the current COVID-19 vaccine distribution inequality and propose that pharmaceutical companies re-examine their roles and social responsibilities.
Avian Cell Line DuckCelt®-T17 Is an Efficient Production System for Live-Attenuated Human Metapneumovirus Vaccine Candidate Metavac®
Authors: Chupin Caroline , Pizzorno Andrés , Traversier Aurélien , Brun Pauline , Ogonczyk-Makowska Daniela , Padey Blandine , Milesi Cédrine , Dulière Victoria , Laurent Emilie , Julien Thomas , Galloux Marie , Lina Bruno , Eléouët Jean-François , Moreau Karen , Hamelin Marie-Eve , Terrier Olivier , Boivin Guy , Dubois Julia , Rosa-Calatrava Manuel
Published: October 2021

The development of a live-attenuated vaccine (LAV) for the prevention of human metapneumovirus (HMPV) infection is often hampered by the lack of highly efficient and scalable cell-based production systems that support eventual global vaccine production. Avian cell lines cultivated in suspension compete with traditional cell platforms used for viral vaccine manufacture. We investigated whether the DuckCelt®-T17 avian cell line (Vaxxel), previously described as an efficient production system for several influenza strains, could also be used to produce a new HMPV LAV candidate (Metavac®, SH gene-deleted A1/C-85473 HMPV). To that end, we characterized the operational parameters of MOI, cell density, and trypsin addition to achieve the optimal production of Metavac®, and demonstrated that the DuckCelt®-T17 cell line is permissive and well-adapted to the production of the wild-type A1/C-85473 HMPV and the Metavac® vaccine candidate. Moreover, our results confirmed that the LAV candidate produced in DuckCelt®-T17 cells conserves its advantageous replication properties in LLC-MK2 and 3D-reconstituted human airway epithelium models, and its capacity to induce efficient neutralizing antibodies in a BALB/c mouse model. Our results suggest that the DuckCelt®-T17 avian cell line is a very promising platform for the scalable in-suspension serum-free production of the HMPV-based LAV candidate Metavac®.
Seroepidemiology of Measles, Mumps, Rubella and Varicella in Italian Female School Workers: A Cross-Sectional Study
Authors: Frau Nicola , Meloni Federico , Fostinelli Jacopo , Portas Laura , Portoghese Igor , Sala Emma , Pilia Ilaria , Lecca Luigi Isaia , De Palma Giuseppe , Campagna Marcello
Published: October 2021

Background: Determining the proportion of susceptible workers can represent a first step to the biological risk assessment related to measles, mumps, rubella and varicella exposure. This study aimed to assess the immunity against measles, mumps, rubella and varicella viruses in a cohort of female school workers. Methods: A cross-sectional seroepidemiological study in a sample of 263 school workers undergoing routine annual workplace health surveillance program was conducted. As part of the health surveillance program, serum samples were collected and tested for measles, mumps, rubella and varicella IgG antibodies. Results: Overall seropositivity was 90.5%, 85.2%, 94.7% and 97.3% for measles, mumps, rubella and varicella, respectively. In relation to mumps occupation-specific seropositivity, a statistically significant difference was observed, showing the lowest prevalence of protected individuals in other occupation groups. Moreover, in relation to rubella, school workers born in Centre Italy had the lowest seropositivity of protective antibodies and the difference between groups was statistically significant. Measles and rubella seropositivity showed a significant decrease after 2015. Conclusions: This study showed a relevant proportion of school workers susceptible to the aforementioned diseases. These results highlighted the need for proper health surveillance and immunological controls in school workers, especially for females, and provided useful insights to policymakers to select effective strategies aimed at containing the risk of vaccine-preventable diseases at schools.
Genetic Insights into the Middle East Respiratory Syndrome Coronavirus Infection among Saudi People
Authors: Abuelizz Hatem , AlRasheed Maha , Alhoshani Ali , Alhawassi Tariq
Published: October 2021

Background: The Middle East respiratory syndrome coronavirus (MERS-CoV) was isolated for the first time in Saudi Arabia from a patient suffering from atypical pneumonia. The Saudi Genome database was built by King Abdulaziz Medical City via the next-generation sequencing of 7000 candidates. Method: A large list of point mutations were reported in the region of the dipeptidyl peptidase 4 (DPP4) gene. The DPP4 amino acid residues correlated to MERS-CoV entry and the site of activity of DPP4 inhibitors was investigated. We retrieved the SNPs (Single-Nucleotide Polymorphism) with a variation frequency of >0.05. Results: SNP 2:162,890,175 and SNP 2:162,891,848 in the intronic region were located within 50 bp of amino acid residues responsible for MERS-CoV entry, amino acids 259–296 and 205–258, respectively. The variation frequency of SNP 2:162,890,175 was 2321 out of 2379 screened individuals. Moreover, mutation of SNP 2:162,891,848, which is located near amino acid residues E205 and E206 (crucial for the activity of DPP4 inhibitors), occurred in 76 out of 2379 screened individuals. Conclusions: Our study shows high variation frequency in the DPP4 region reported in the Saudi Genome database. The identified SNPs are of high significance for MERS-CoV infection in better understanding disease pathogenesis.
A Study of Hepatitis A Seroprevalence in a Paediatric and Adolescent Population of the Province of Florence (Italy) in the Period 2017–2018 Confirms Tuscany a Low Endemic Area
Authors: Zanella Beatrice , Boccalini Sara , Biamonte Massimiliano Alberto , Giorgetti Duccio , Menicacci Marco , Bonito Benedetta , Ninci Alessandra , Tiscione Emilia , Puggelli Francesco , Mereu Giovanna ,   Working Group DHS,   Working Group AOUMeyer,   Working Group AUSLTC, Bonanni Paolo , Bechini Angela
Published: October 2021

Background: Italy is considered an area with very low HAV (hepatitis A virus) endemicity. Currently in Italy the anti-HAV vaccine is recommended only for specific risk groups and there is no universal vaccination program. The aim of this study was to assess the level of immunity against hepatitis A in a sample of children and adolescents from the province of Florence. Methods: A total of 165 sera were collected from subjects aged 1 to 18 years, proportionally selected according to the general population size and stratified by age and sex. A qualitative evaluation of anti-HAV antibodies was performed using the enzyme-linked immunosorbent assay (ELISA). Anamnestic and vaccination status data were also collected. Results: Our study showed a hepatitis A seroprevalence of 9.1% in the enrolled population. A statistically significant difference in the prevalence of anti-HAV was found between Italian and non-Italian subjects. About half of the population having anti-HAV antibodies was reported to be vaccinated, and no cases of hepatitis A were found. Conclusions: The data from our study confirmed Tuscany as an area with low HAV endemicity and showed that hepatitis A seroprevalence is significantly higher in foreign children and adolescents. The presence of more seropositive subjects than those vaccinated was probably due to a natural immunization achieved through a subclinical infection and/or to underreporting of the surveillance systems.
Emerging SARS-CoV-2 Variants: A Review of Its Mutations, Its Implications and Vaccine Efficacy
Authors: Ramesh Sindhu , Govindarajulu Manoj , Parise Rachel S. , Neel Logan , Shankar Tharanath , Patel Shriya , Lowery Payton , Smith Forrest , Dhanasekaran Muralikrishnan , Moore Timothy
Published: October 2021

The widespread increase in multiple severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants is causing a significant health concern in the United States and worldwide. These variants exhibit increased transmissibility, cause more severe disease, exhibit evasive immune properties, impair neutralization by antibodies from vaccinated individuals or convalescence sera, and reinfection. The Centers for Disease Control and Prevention (CDC) has classified SARS-CoV-2 variants into variants of interest, variants of concern, and variants of high consequence. Currently, four variants of concern (B.1.1.7, B.1.351, P.1, and B.1.617.2) and several variants of interests (B.1.526, B.1.525, and P.2) are characterized and are essential for close monitoring. In this review, we discuss the different SARS-CoV-2 variants, emphasizing variants of concern circulating the world and highlight the various mutations and how these mutations affect the characteristics of the virus. In addition, we discuss the most common vaccines and the various studies concerning the efficacy of these vaccines against different variants of concern.
Preparedness Plan for the Water Supply Infrastructure during Water Terrorism—A Case Study from Irbid, Jordan
Authors: Hindiyeh Muna , Albatayneh Aiman , Tarawneh Rashed , Suleiman Suzan , Juaidi Adel , Abdallah Ramez , Jellali Salah , Jeguirim Mejdi
Published: October 2021

Terrorism is a crucial danger to the world, especially the Middle East. As Jordan is amidst countries with armed conflicts, its natural resources (in particularly, water resources) are vulnerable to terrorist attacks. In this paper, possible biological and chemical weapons that can be used as intimidation, along with their threats, vulnerabilities, and inactivation methods, concerning water treatment processes in the municipality of Irbid, are studied. Irbid is the second largest city in terms of population in Jordan, after the capital city of Amman, and it is the nearest governorate to Syria borders that is considered a war zone. After conducting risk assessment that takes into consideration criticality, threats, and vulnerability, it appears that only one of the eight units, which is the Makhraba pumping station, along with the Bushra water tank, were identified as medium risk. The other units have treatment processes and proper precautions that are able to inactivate or prevent any possible contamination. A response plan should be set by developing a telemetry system with specific sensors that can detect any sudden and unacceptable threats to the water quality and that has the ability to shut down the concerned units automatically.
Some Observations on Phytoplankton Community Structure, Dynamics and Their Relationship to Water Quality in Five Santiago Island Reservoirs, Cape Verde
Authors: Morais Manuela , Penha Alexandra Marchã , Novais Maria Helena , Landim Leonel , Victória Sónia Silva , Morales Eduardo A. , Barbosa Luciana Gomes
Published: October 2021

Reservoirs provide valuable services to human beings, especially in arid, semi-arid, and Mediterranean regions affected by water scarcity. The present effort aims to study the environmental descriptors of variation and the main factors influencing phytoplankton composition, structure, and diversity in five reservoirs in Santiago Island, Cape Verde, a region affected by water availability. Five campaigns took place from 2016 to 2020 to sample phytoplankton and measure environmental variables according to standard analytical methodologies. Environmental results (17 water physicochemical variables, air temperature, and precipitation) revealed that reservoirs differ in the geological influence variables. The high levels of P and N in water seem to be related to Land Use/Land Cover and are responsible for water-quality degradation. Cyanobacteria dominated the phytoplankton community and posed high risk levels, especially considering that the identified taxa are potential producers of different toxins. Taxa responsible for this dominance were not the same in all reservoirs, emphasizing the dominant role of local habitat factors on community composition and diversity. Overall, the results reveal the importance of defining integrated management plans/strategies for the set of five studied reservoirs, since the processes influencing variation in the phytoplankton community are temporal-scale dependent, with similar biogeographic patterns.
Evaluation and Optimization of Low Impact Development Designs for Sustainable Stormwater Management in a Changing Climate
Authors: Abduljaleel Yasir , Demissie Yonas
Published: October 2021

The increasing intensity and frequency of extreme storms pose a growing challenge to stormwater management in highly urbanized areas. Without an adequate and appropriate stormwater system, the storms and associated floods will continue to cause significant damage to infrastructure and loss of life. Low Impact Development (LID) has become an emerging alternative to the traditional stormwater system for stormwater management. This study evaluates and optimizes applications of different combinations of LIDs to minimize flows from a catchment under past and future storm conditions. The Storm Water Management Model (SWMM), forced by observed and downscaled precipitation from Coupled Model Intercomparison Project phase 6 (CMIP6), was used to simulate the runoff and apply the LIDs in the Renton City, WA. The final results show that the performance of LIDs in reducing total runoff volume varies with the types and combinations of LIDs utilized. A 30% to 75% runoff reduction was achieved for the past and future 50 year and 100 year storms. The study demonstrates the effectiveness of LID combinations with conventional stormwater systems to manage the future runoff in the study area, which is expected to increase by 26.3% in 2050.
Identification of Natural and Anthropogenic Geochemical Processes Determining the Groundwater Quality in Port del Comte High Mountain Karst Aquifer (SE, Pyrenees)
Authors: Herms Ignasi , Jódar Jorge , Soler Albert , Lambán Luís Javier , Custodio Emilio , Núñez Joan Agustí , Arnó Georgina , Parcerisa David , Jorge-Sánchez Joan
Published: October 2021

The Port del Comte Massif (SE, Pyrenees) contains one of the most important vulnerable and strategic karst aquifers for supplying freshwater to the city of Barcelona (Spain). It is a fragile system, whose possible environmental impact is highly conditioned by land use. To improve the hydrogeological knowledge of the system, between September 2013 and October 2015, a detailed fieldwork was carried out for the revision of the geological model, the inventory of water points, and the in situ physico-chemical characterization on major elements and isotopes of up to a total of 43 springs, as well as precipitation water. This paper focuses on the characterization of the geochemical processes that allow explanation of the observed chemical variability of groundwater drained by the pristine aquifer system to determine the origin of salinity. The results show that the main process is the dissolution of calcite and dolomite, followed by gypsum and halite, and a minor cation exchange-like process. Sulfur and oxygen isotopes from dissolved sulfate in the studied springs point out a geogenic origin related to the dissolution of gypsum from Triassic and Tertiary materials, and that the contribution from anthropogenic sources, like fertilizers, is lower. Nitrate in groundwater is not an important issue, with a few localized cases related with agricultural activities. The multidisciplinary approach has allowed the development of a consistent hydrogeological conceptual model of the functioning of the aquifer system, which can be replicated in other places to understand the geogenic character of the hydrogeochemistry.
Impacts of Climate and Anthropogenic Activities on Streamflow Regimes in the Beiluo River, China
Authors: Xie Zhibo , Mu Xingmin , Gao Peng , Wu Changxue , Qiu Dexun
Published: October 2021

Quantitatively assessing the characteristics of river streamflow variation and conducting research on attribution identification are the basis for formulating climate-change response strategies and rational use of water resources. Based on the daily streamflow data of the Zhuangtou Hydrological Station in 1970–2018, this paper analyzes the streamflow changes in the Beiluo River Basin and studies the impact of climate change and anthropogenic activities on the streamflow in this basin. A non-parametric Mann–Kendall test and Pettitt’s test were used to determine the trend and detect abrupt changes of streamflow and baseflow. The method based on precipitation and potential evapotranspiration, as well as the double-mass curve of precipitation–streamflow, was established to evaluate the impact of climate change and non-climate factors on annual streamflow. The results reveal a statistically significant downward trend (p = 0.01) in both annual streamflow and baseflow, with the abrupt point year in 1994 and 1988, respectively. When comparing to a modest declining trend in annual average precipitation, we see that the temperature showed a significant upward trend (p = 0.01), whose abrupt point year was 1996. Under the policy of returning farmland to forest, land-use analysis shows that the area of farmland had decreased by 222.4 km2, of which 31.4% was mainly converted into the forestland. By the end of 2015, the area of forestland had increased by 123.4 km2, which has largely caused streamflow decrease. For the method based on precipitation and potential evapotranspiration, climate change contributed 43.7% of the annual streamflow change, and human activities (mainly refers to LUCC) contributed 56.3%. For the DMC of precipitation–streamflow, the precipitation contributed 9.4%, and non-precipitation factors (mainly refers to human activities) contributed 90.6%, and human activities played a more vital part in driving streamflow reduction in different decades, with a contribution rate of more than 70%. This study is of great practical significance to the planning, management, development and utilization of water resources in basins.