bluuebunny/crossref_metadata_2025 huggingface dataset:
Variations in Leaf Traits Modulate Plant Vegetative and Reproductive Phenological Sequencing Across Arid Mediterranean Shrublands
Authors: Navarro Teresa , Hidalgo-Triana Noelia
Published: August 2021

Structural and nutrient traits of a leaf are important for understanding plant ecological strategies (e.g., drought avoidance). We studied the specific leaf area (SLA), leaf carbon content (LCC), leaf nitrogen content (LNC), leaf phosphorous content (LPC), and the phenophase sequence index (PSI) in 126 Mediterranean perennial species from predesert (SMS) and semiarid (SaMS) to subalpine (SAS), alpine cushion (AcS), and oro-Mediterranean (AjS) shrublands, which represent eight functional groups (evergreen and deciduous trees, evergreen large and half shrubs, deciduous large and half shrubs, succulents and perennial herbs). We analyzed the variation and relationships between leaf traits and PSI among shrublands, functional groups, and within species with drought-avoidance mechanisms. SLA variation of 20–60% could be ascribed to differences between functional groups and only 38–48% to different shrublands increasing from the predesert to the alpine. Alpine species display low PSI and N:P and high SLA, LNC, LPC, LCC, and C:N. On the contrary, predesert and semiarid showed high PSI and low SLA. SLA mediates the vegetative and reproductive phenological plant sequencing, high SLA is often associated with the overlapping in growth and reproductive phenophases with a seasonal reduction of vegetative growth, whereas low SLA is associated with vegetative and reproductive sequencing and a seasonal extension of vegetative growth. Species with drought-avoidance mechanisms (e.g., semideciduous species) contribute to an increase in the mean values of the SLA and LNC because these species show similar leaf and phenological patterns as the deciduous (high SLA and LNC and low PSI). The N:P indicates that only the alpine shrublands could present P limitations. The positive correlations between SLA and LPC and LNC and LPC (leaf economic spectrum) and the negative correlation between SLA and C:N were consistently maintained in the studied arid Mediterranean shrublands.
The Alkali Tolerance of Broomcorn Millet (Panicum miliaceum L.) at the Germination and Seedling Stage: The Case of 296 Broomcorn Millet Genotypes
Authors: Ma Qian , Wu Caoyang , Liang Shihan , Yuan Yuhao , Liu Chunjuan , Liu Jiajia , Feng Baili
Published: August 2021

Broomcorn millet (BM), one of the earliest domesticated cereal crops originating in northern China, can tolerate extreme conditions, such as drought and high temperatures, which are prevalent in saline-alkali, arid, and barren landscapes. However, its adaptive mechanism to alkali stress is yet to be comprehensively understood. In this study, 80 and 40 mM standard alkali stress concentrations were used to, respectively, evaluate the alkali tolerance at the germination and seedling stages of 296 BM genotypes. Principal component analysis (PCA), Pearson's correlation analysis, and F-value comprehensive analysis were performed on the germination parameters (germination potential, germination index, germination rate, vigor index, root length/weight, sprout length/weight, and alkali damage rate). Based on their respective F-values, the BM genotypes were divided into five categories ranging from highly alkali resistant to alkali sensitive. To study the response of seedlings to alkaline stress, we investigated the phenotypic parameters (plant height, green leaf area, biomass, and root structure) of 111 genotypes from the above five categories. Combining the parameters of alkali tolerance at the germination and seedling stages, these 111 genotypes were further subdivided into three groups with different alkali tolerances. Variations in physiological responses of the different alkali-tolerant genotypes were further investigated for antioxidant enzyme activity, soluble substances, malondialdehyde (MDA) content, electrolyte leakage rate, and leaf structure. Compared with alkali-sensitive genotypes, alkali-tolerant genotypes had high antioxidant enzyme activity and soluble osmolyte content, low MDA content and electrolyte leakage rate, and a more complete stomata structure. Taken together, this study provides a comprehensive and reliable method for evaluating alkali tolerance and will contribute to the improvement and restoration of saline-alkaline soils by BM.
Proteome Analysis of Condensed Barley Mitotic Chromosomes
Authors: Perutka Zdeněk , Kaduchová Kateřina , Chamrád Ivo , Beinhauer Jana , Lenobel René , Petrovská Beáta , Bergougnoux Véronique , Vrána Jan , Pecinka Ales , Doležel Jaroslav , Šebela Marek
Published: August 2021

Proteins play a major role in the three-dimensional organization of nuclear genome and its function. While histones arrange DNA into a nucleosome fiber, other proteins contribute to higher-order chromatin structures in interphase nuclei, and mitotic/meiotic chromosomes. Despite the key role of proteins in maintaining genome integrity and transferring hereditary information to daughter cells and progenies, the knowledge about their function remains fragmentary. This is particularly true for the proteins of condensed chromosomes and, in particular, chromosomes of plants. Here, we purified barley mitotic metaphase chromosomes by a flow cytometric sorting and characterized their proteins. Peptides from tryptic protein digests were fractionated either on a cation exchanger or reversed-phase microgradient system before liquid chromatography coupled to tandem mass spectrometry. Chromosomal proteins comprising almost 900 identifications were classified based on a combination of software prediction, available database localization information, sequence homology, and domain representation. A biological context evaluation indicated the presence of several groups of abundant proteins including histones, topoisomerase 2, POLYMERASE 2, condensin subunits, and many proteins with chromatin-related functions. Proteins involved in processes related to DNA replication, transcription, and repair as well as nucleolar proteins were found. We have experimentally validated the presence of FIBRILLARIN 1, one of the nucleolar proteins, on metaphase chromosomes, suggesting that plant chromosomes are coated with proteins during mitosis, similar to those of human and animals. These results improve significantly the knowledge of plant chromosomal proteins and provide a basis for their functional characterization and comparative phylogenetic analyses.
The Power and Peril of Precise vs. Round Health Message Interventions to Increase Stair Use
Authors: Krull Sebastian , Boecker Lea , Loschelder David D.
Published: August 2021

Taking the stairs vs. an elevator generate benefits for the individual by increasing overall physical activity, health, and wellbeing. In the present paper, we report two pre-registered field intervention studies that examine how health message interventions can motivate individuals to change their behavior. We empirically contrasted opposing predictions from the literature as to whether numerically round (60.00%) or precise (61.87%) health messages are more effective in causing people to use the stairs over taking the elevator. Both interventions were compared to a control condition (no-health message). Contrary to our hypotheses and extant findings, both intervention studies did not produce a significant positive effect of the interventions relative to the control condition. In recent years such null findings have received increasingly more appreciation, particularly in the light of evident downsides of file-drawered studies. We discuss a number of moderating factors that may determine when and why nudging interventions are (in-) effective (e.g., a priori behavioral prevalence, pre-established habits, ceiling effects, and building infrastructure), as well as limitations and avenues for future research.
Investigating Dishonesty-Does Context Matter?
Authors: Waeber Aline
Published: August 2021

This paper introduces frame-specific randomization devices to vary the situational context of an online lying experiment. Participants are asked to report outcomes of random draws from two different sources of uncertainty—decimals of the value of a stock index or a neutrally framed random number generator. The findings show that the frame-specific randomization device is not prone to the social norm effects documented in the literature. Because different environments can evoke different norms, I replicate the experiment in the more constrained setting of a traditional physical laboratory revealing no systematic differences in behavior. Furthermore, I am not able to show that participants who take longer to report are more honest and this is specific to the physical laboratory environment. Finally, the findings reveal gender differences in honesty depending on the environment—males are more honest when they participate in the laboratory as opposed to online.
Positive Youth Development and Mental Well-Being in Late Adolescence: The Role of Body Appreciation. Findings From a Prospective Study in Norway
Authors: Urke Helga Bjørnøy , Holsen Ingrid , Larsen Torill
Published: August 2021

Although a vulnerable period, adolescence is a time of great potential for healthy development. Understanding factors that contribute to mental well-being in this period is of great importance to facilitate for healthy development. During the adolescence period the body goes through rapid and considerable changes, and the focus on body appearance and perfection is substantial at societal, media, and peer level. In this study, we investigated the association between dimensions characterizing positive youth development, and mental well-being among Norwegian adolescents 16–17 years 1 year later, and whether this association was mediated by degree of body appreciation. We further tested whether the indirect and direct paths between positive youth development dimensions and mental well-being were moderated by gender. We used data from the first and second follow-up of the control arm of the COMPLETE study, a cluster randomized controlled trial in upper secondary schools in Norway. Findings showed that positive youth development in grade 1, as measured by both a combined global PYD and the dimensions competence, and connection were significantly associated with mental well-being in grade 2 in models adjusted for mental well-being in grade 1, gender and perceived family affluence. Also, body appreciation in grade 1 significantly predicted mental well-being in grade 2 in models including each of global PYD, competence, connection, character, and caring dimensions. Contrary to our hypothesis, no mediating effects of body appreciation were observed, and no moderation of indirect effects by gender were observed.
Children's (Mis)understanding of the Balance Beam (Online Edition)
Authors: Filion Virginie M. L. , Sirois Sylvain
Published: August 2021

The balance-scale task, proposed by Inhelder and Piaget, illustrates children understanding of weight-distance relationships. Piaget used the clinical interview method in order to investigate children's reasoning. Over the last five decades, Siegler's Rule-Assessment Approach has been used to explain children reasoning in the balance-scale task according to rules children would use to solve the task. However, this approach does not take into account some key perceptual properties of the task. This study evaluates whether different task demands would alter children's errors. Forty children (twenty children aged 4–5 years and twenty children aged 9–10 years) predicted the movement of both arms of 16 balance-scale problems administered online. Nine 4–5-year-olds produced non-plausible responses whereas none of the 9–10-year-olds provided non-plausible responses. These results seem to indicate a basic misunderstanding of the scale from some younger children, one that eludes traditional measures used with this task.
Harnessing the Fifth Element of Distributional Statistics for Psychological Science: A Practical Primer and Shiny App for Measures of Statistical Inequality and Concentration
Authors: Tran Ulrich S. , Lallai Taric , Gyimesi Marton , Baliko Josef , Ramazanova Dariga , Voracek Martin
Published: August 2021

Although distributional inequality and concentration are important statistical concepts in many research fields (including economics, political and social science, information theory, and biology and ecology), they rarely are considered in psychological science. This practical primer familiarizes with the concepts of statistical inequality and concentration and presents an overview of more than a dozen useful, popular measures of inequality (including the Gini, Hoover, Rosenbluth, Herfindahl-Hirschman, Simpson, Shannon, generalized entropy, and Atkinson indices, and tail ratios). Additionally, an interactive web application (R Shiny) for calculating and visualizing these measures, with downloadable output, is described. This companion Shiny app provides brief introductory vignettes to this suite of measures, along with easy-to-understand user guidance. The Shiny app can readily be used as an intuitively accessible, interactive learning and demonstration environment for teaching and exploring these methods. We provide various examples for the application of measures of inequality and concentration in psychological science and discuss venues for further development.
Impact of Entrepreneurial Education, Mindset, and Creativity on Entrepreneurial Intention: Mediating Role of Entrepreneurial Self-Efficacy
Authors: Jiatong Wang , Murad Majid , Bajun Fu , Tufail Muhammad Shahid , Mirza Farhan , Rafiq Muhammad
Published: August 2021

This study aimed to investigate the direct influence of entrepreneurial education, entrepreneurial mindset, and creativity on the entrepreneurial intention with the indirect role of entrepreneurial self-efficacy. This study applied the structural equation model technique using AMOS software to verify the hypothesis relationships. This study collected self-administered survey data from 365 university students of Jiangsu and Zhejiang province of China. The findings indicated that entrepreneurial education, entrepreneurial mindset, and creativity have a positive and significant influence on entrepreneurial intention. Moreover, results revealed that entrepreneurial self-efficacy partially mediates in the relationship between entrepreneurial education, entrepreneurial mindset, and creativity on entrepreneurial intention. Further implications and limitations are also discussed in this article.
Reference Values and Related Factors for Peak Expiratory Flow in Middle-Aged and Elderly Chinese
Authors: Ji Chao , Xia Yang , Dai Huixu , Zhao Zhiying , Liu Tiancong , Tong Shuhui , Zhang Xiaohang , Zhao Yuhong
Published: August 2021

Background: Peak expiratory flow (PEF), as an essential index used for screening and monitoring asthma, chronic obstructive pulmonary disease, and respiratory mortality especially in the elderly, is recommended for low-resource settings in low- and middle-income countries. However, few studies have focused on the reference of PEF in China, especially in middle-aged and elderly people. Thus, this study aimed to determine age- and sex-specific reference values of PEF in the middle-aged and elderly Chinese population.

Methods: There were 8,914 participants who were included for risk factor analysis and 5,498 participants included for reference value analysis. The PEF was measured using a peak flow meter in liters per minute. The distributions of standardized PEF terciles stratified by sex and age were reported. Multiple linear regression analysis was used to determine the associations between risk factors and PEF.

Results: The PEF was higher in men than women across all age subgroups. The value of PEF decreased with age in both men and women. Height, weight, handgrip strength, and residence in rural were positively associated with PEF. Age and smoking status were negatively associated with PEF significantly in both men and women (P < 0.05). The mean PEF values were 367.10 and 253.00 L/min for men and women, respectively. Meanwhile, the prevalence of low PEF was 3.94 and 3.32% for men and women, respectively.

Conclusions: Age- and sex-specific centiles of standardized PEF for the middle-aged and elderly Chinese population were estimated. The reference values for low PEF could provide reference standards for epidemiological studies and clinical practices in the future. Interventions to improve lung functions or to prevent respiratory disease should be paid more attention to factors associated with PEF.
Lessons Learned From COVID-19 Contact Tracing During a Public Health Emergency: A Prospective Implementation Study
Authors: Shelby Tyler , Schenck Christopher , Weeks Brian , Goodwin Justin , Hennein Rachel , Zhou Xin , Spiegelman Donna , Grau Lauretta E. , Niccolai Linda , Bond Maritza , Davis J. Lucian
Published: August 2021

Background: Contact tracing is a core element of the public health response to emerging infectious diseases including COVID-19. Better understanding the implementation context of contact tracing for pandemics, including individual- and systems-level predictors of success, is critical to preparing for future epidemics.

Methods: We carried out a prospective implementation study of an emergency volunteer contact tracing program established in New Haven, Connecticut between April 4 and May 19, 2020. We assessed the yield and timeliness of case and contact outreach in reference to CDC benchmarks, and identified individual and programmatic predictors of successful implementation using multivariable regression models. We synthesized our findings using the RE-AIM implementation framework.

Results: Case investigators interviewed only 826 (48%) of 1,705 cases and were unable to reach 545 (32%) because of incomplete information and 334 (20%) who missed or declined repeated outreach calls. Contact notifiers reached just 687 (28%) of 2,437 reported contacts, and were unable to reach 1,597 (66%) with incomplete information and 153 (6%) who missed or declined repeated outreach calls. The median time-to-case-interview was 5 days and time-to-contact-notification 8 days. However, among notified contacts with complete time data, 457 (71%) were reached within 6 days of exposure. The least likely groups to be interviewed were elderly (adjusted relative risk, aRR 0.74, 95% CI 0.61–0.89, p = 0.012, vs. young adult) and Black/African-American cases (aRR 0.88, 95% CI 0.80–0.97, pairwise p = 0.01, vs. Hispanic/Latinx). However, ties between cases and their contacts strongly influenced contact notification success (Intraclass Correlation Coefficient (ICC) 0.60). Surging caseloads and high volunteer turnover (case investigator n = 144, median time from sign-up to retirement from program was 4 weeks) required the program to supplement the volunteer workforce with paid public health nurses.

Conclusions: An emergency volunteer-run contact tracing program fell short of CDC benchmarks for time and yield, largely due to difficulty collecting the information required for outreach to cases and contacts. To improve uptake, contact tracing programs must professionalize the workforce; better integrate testing and tracing services; capitalize on positive social influences between cases and contacts; and address racial and age-related disparities through enhanced community engagement.
Safety Profile of Caesalpinia spinosa Aqueous Extract Tested in Oreochromis niloticus Toward Its Application in Dermocosmetics
Authors: Salirrosas David , Reategui-Pinedo Nataly , Crespo Jan Pier , Sánchez-Tuesta Linda , Arqueros Mónica , Cabrera Angelita , Martinez Renata Miliani , Ayala Carmen , Baby André Rolim , Prieto Zulita Adriana
Published: August 2021

Herbal extracts have been widely used in dermocosmetics as a source of biomolecules and also as a natural claim. Fruits from Caesalpinia spinosa show great potential for their polyphenolic content, preservative, and film-forming features, as previously reported in specialized literature; however, the toxicity requires investigation. We explored Oreochromis niloticus (tilapia) in larval, alevins, and juvenile stages to evaluate the ex vivo and in vivo genotoxicity and in vivo acute and chronic toxicity of C. spinosa aqueous extract in different concentrations. Cytotoxicity, animal behavior, morphological deformities, and DNA damage were evaluated. Our results showed genotoxic effect in ex vivo tests, but no DNA damage in in vivo erythrocytes. We suggest a mechanism of cell permeability involved in the toxicity of C. spinosa aqueous extract. Internal validation showed the feasibility of O. niloticus applied for toxicity evaluation. Further studies could contribute for better understanding the uses and safety of C. spinosa in cosmetics and topical pharmaceutical products.
Case Report: Positive Outcome of a Suspected Drug-Associated (Immune Mediated) Reaction in a 4-Year-Old Male French Bulldog
Authors: Lecru Line-Alice , Combarros Daniel , Castilla-Castaño Eloy , Delverdier Maxence , Cadiergues Marie-Christine , Pressanti Charline
Published: August 2021

Toxic epidermal necrolysis (TEN) is a rare and severe life-threatening syndrome characterized by apoptosis of keratinocytes resulting in devitalization of the epidermis affecting more than 30% of skin surface. In humans and animals, this condition is mostly triggered by drugs. Identification of the putative agent and its withdrawal are crucial to successful management of a patient with TEN. In this case study, we report the clinical features, histopathological findings and management of a dog with TEN. A 4-year-old intact male French bulldog presented with acute onset of severe lethargy and cutaneous ulcerations on the footpads, scrotum, and hind limbs associated with marked pain. A Stevens-Johnson syndrome/TEN was suspected and drugs, especially beta-lactams, were withdrawn. Histopathology confirmed the diagnosis of epidermal necrosis. Advanced supportive therapy, pain management and skin care led to rapid remission. Early identification and removal of the suspected medication was crucial to improving TEN prognosis in this dog. Antibiotics (penicillin, ampicillin, cephalexin, and sulfonamides) are frequently involved in adverse cutaneous reactions in dogs. Ideal treatment remains elusive is humans and dogs and this disease has a poor prognosis. Supportive care combined with pain management and treatment of the cutaneous ulcerations is essential.
Transmission Dynamics of Corynebacterium spp. Within Two Danish Dairy Cattle Herds
Authors: Kirkeby Carsten , Halasa Tariq , Farre Michael , Chehabi Galal Nazih , Græsbøll Kaare
Published: August 2021

Intramammary infections (IMI) can cause mastitis, a prevalent and costly infectious disease in dairy cattle worldwide. The IMI is caused by a range of bacteria, including Corynebacterium spp. Knowledge of the transmission dynamics of pathogens is generally sparse but essential to support decision-making; such as input to bioeconomic models. In this observational study, we explored the transmission dynamics of Corynebacterium spp. in two different Danish dairy cattle herds by testing monthly quarter-level milk samples of all lactating cows for 1 year. We estimated the prevalence for herd 1 and 2 to 24 and 11.7%, respectively, and the mean quarter-level incidence to be 8 and 6.5% per month, respectively. We compared a model for indirect transmission via the environment with a model with the direct contagious transmission and found that the latter model best explained the data. We estimated the daily mean quarter-level transmission rate to be 0.016 and 0.018 cases/quarter-day for herd 1 and 2, respectively. The mean recovery rate was 0.012 and 0.016 for herd 1 and 2, respectively. Consequently, the basic reproduction number for herd 1 and 2 was 1.27 and 1.10, respectively. This study highlights that Corynebacterium spp. can be prevalent within a herd and transmit directly between cows. Thus, future studies should investigate cost-effective control measures against Corynebacterium spp.
Comparative Analysis of Recurrent Neural Networks in Stock Price Prediction for Different Frequency Domains
Authors: Dey Polash , Hossain Emam , Hossain Md. Ishtiaque , Chowdhury Mohammed Armanuzzaman , Alam Md. Shariful , Hossain Mohammad Shahadat , Andersson Karl
Published: August 2021

Investors in the stock market have always been in search of novel and unique techniques so that they can successfully predict stock price movement and make a big profit. However, investors continue to look for improved and new techniques to beat the market instead of old and traditional ones. Therefore, researchers are continuously working to build novel techniques to supply the demand of investors. Different types of recurrent neural networks (RNN) are used in time series analyses, especially in stock price prediction. However, since not all stocks’ prices follow the same trend, a single model cannot be used to predict the movement of all types of stock’s price. Therefore, in this research we conducted a comparative analysis of three commonly used RNNs—simple RNN, Long Short Term Memory (LSTM), and Gated Recurrent Unit (GRU)—and analyzed their efficiency for stocks having different stock trends and various price ranges and for different time frequencies. We considered three companies’ datasets from 30 June 2000 to 21 July 2020. The stocks follow different trends of price movements, with price ranges of $30, $50, and $290 during this period. We also analyzed the performance for one-day, three-day, and five-day time intervals. We compared the performance of RNN, LSTM, and GRU in terms of R2 value, MAE, MAPE, and RMSE metrics. The results show that simple RNN is outperformed by LSTM and GRU because RNN is susceptible to vanishing gradient problems, while the other two models are not. Moreover, GRU produces lesser errors comparing to LSTM. It is also evident from the results that as the time intervals get smaller, the models produce lower errors and higher reliability.
Design and Analysis of a Stepping Piezoelectric Actuator Free of Backward Motion
Authors: Yang Xiaofeng , Tang Jinyan , Guo Wenxin , Huang Hu , Fan Haoyin , Liu Jiahui , Li Tao
Published: August 2021

Although the stick-slip principle has been widely employed for designing piezoelectric actuators, there still exits an intrinsic drawback, i.e., the backward motion, which significantly affects its output performances and applications. By analyzing the generation mechanism of backward motion in stick-slip piezoelectric actuators, the elliptical trajectory was employed to design a novel stepping piezoelectric actuator free of backward motion. Accordingly, a prototype of piezoelectric actuator was designed, which utilized a flexure hinge mechanism and two vertically arranged piezoelectric stacks to generate the required elliptical trajectory. The compliance matrix method was used to theoretically analyze the flexure hinge mechanism. The theoretical and measured elliptical trajectories under various phase differences were compared, and the phase difference of 45° was selected accordingly. Under a critical relative gap, output performances of the actuator working under the elliptical trajectory were characterized, and then compared with that obtained under the normal stick-slip driving principle. Experimental results indicated that forward and reverse stepping displacement with completely suppressed backward motion could be achieved when employing the elliptical trajectory, verifying its feasibility. This study provides a new strategy for designing a stepping piezoelectric actuator free of backward motion.
VaCRK2 Mediates Gray Mold Resistance in Vitis amurensis by Activating the Jasmonate Signaling Pathway
Authors: Li Tinggang , Gao Huanhuan , Tang Xiaoning , Gong Dongying
Published: August 2021

Cysteine-rich receptor-like kinases (CRKs) are ubiquitous plant receptor-like kinases, which play a significant role in plant disease resistance. Gray mold is an economically important disease of grapes caused by Botrytis cinerea. However, CRK genes and their function in gray mold disease resistance in grapes have not been elucidated. This study aimed to identify and characterize CRKs in grapes and determine their role in gray mold resistance. Four CRKs were identified in Vitis amurensis and named VaCRK1–VaCRK4 according to their genomic distribution. The four VaCRKs were ectopically expressed in Arabidopsis thaliana to study their function in defense response against B. cinerea. Heterologous expression of VaCRK2 in A. thaliana conferred resistance to B. cinerea. VaCRK2 expression in gray mold-resistant grape cultivar increased significantly after B. cinerea inoculation and methyl jasmonate treatment. Furthermore, the expression of jasmonic acid (JA) signaling pathway-related genes in VaCRK2 overexpression lines of A. thaliana was significantly increased after B. cinerea inoculation, leading to the upregulation of pathogenesis-related (PR) genes and reactive oxygen species (ROS) accumulation. Overall, these results suggest that VaCRK2 confers resistance to B. cinerea by activating PR gene expression and oxidative burst through the JA signaling pathway.
The Genetic Diversity and Population Structure of Different Geographical Populations of Bottle Gourd (Lagenaria siceraria) Accessions Based on Genotyping-by-Sequencing
Authors: Contreras-Soto Rodrigo , Salvatierra Ariel , Maldonado Carlos , Mashilo Jacob
Published: August 2021

Lagenaria siceraria (Molina) Standl is an important horticultural and medicinal crop grown worldwide in the food and pharmaceutical industries. The crop exhibits extensive phenotypic and genetic variation useful for cultivar development targeting economic traits; however, limited genomic resources are available for effective germplasm characterization into breeding and conservation strategies. This study determined the genetic relationships and population structure in a collection of different accessions of bottle gourd derived from Chile, Asia, and South Africa by using single-nucleotide polymorphism (SNP) markers and mining of simple sequence repeat (SSR) loci derived from genotyping-by-sequencing (GBS) data. The GBS resulted in 12,766 SNPs classified as moderate to highly informative, with a mean polymorphic information content of 0.29. The mean gene diversity of 0.16 indicated a low genetic differentiation of the accessions. Analysis of molecular variance revealed less differentiation between (36%) as compared to within (48%) bottle gourd accessions, suggesting that a random mating system dominates inbreeding. Population structure revealed two genetically differentiated groups comprising South African accessions and an admixed group with accessions of Asian and Chilean origin. The results of SSR loci mining from GBS data should be developed and validated before being used in diverse bottle gourd accessions. The SNPs markers developed in the present study are useful genomic resources in bottle gourd breeding programs for assessing the extent of genetic diversity for effective parental selection and breeding.
Non-Chemical Soil Fumigation for Sustainable Strawberry Production in Southern Italy
Authors: Giovannini Daniela , Brandi Federica , Lanteri Anna Paola , Lazzeri Luca , Maltoni Maria Luigia , Matteo Roberto , Minuto Andrea , Sbrighi Paolo , Stagno Fiorella , Baruzzi Gianluca
Published: August 2021

In intensive strawberry production, monoculture is a common practice worldwide; however, prolonged replanting can cause plant disorders and jeopardize profitable cultivation of this highly valuable crop. To mitigate replanting problems, the strawberry industry is still highly dependent on chemical fumigation. Given the increasing regulatory restrictions and concerns about human and environmental risks from fumigants use, there is a growing interest in the adoption of effective, non-chemical alternatives. Two non-chemical soil fumigation practices, i.e., anaerobic soil disinfestation (ASD) and bio-fumigation with biocide plants (BIOFUM), were tested against chemical fumigation by chloropicrin + 1,3-dichloropropene mixture (STANDARD) and untreated (UNTREAT) control in a 2-year trial established in a commercial strawberry farm in Southern Italy (40°25′ N, 16°42′ E). Overall, the alternative practices provided consistently better results than UNTREAT; whereas, compared to STANDARD, their performance was significantly different in the two years: in 2018/19 season the alternative practices registered a 20% (ASD) and 39% (BIOFUM) marketable yield loss compared to STANDARD, while in the 2019/20 season yield differences were not significant. Although both practices appear promising as eco-friendly alternatives to chemical fumigation, in this short-term trial ASD performed better than BIOFUM both in terms of yield and fruit size, resulting in a more advanced stage for practical adoption.
Individual Nest Site Preferences Do Not Explain Upslope Population Shifts of a Secondary Cavity-Nesting Species
Authors: Abeyta Elisa J. , Bartlow Andrew W. , Hathcock Charles D. , Fair Jeanne M.
Published: August 2021

Geographic ranges of plants and animals are shifting due to environmental change. While some species are shifting towards the poles and upslope in elevation, the processes leading to these patterns are not well known. We analyzed 22 years of western bluebird (Sialia mexicana) data from a large nest box network in northern New Mexico at elevations between 1860 m and 2750 m. This population has shifted to higher elevations over time, but whether this is due to changes in nesting behavior and preference for higher elevation within the population or driven by immigration is unclear. We banded adults and nestlings from nest boxes and examined nesting location and elevation for individual birds captured two or more times. Most recaptured birds nested at the same nest boxes in subsequent years, and the number of birds that moved upslope did not significantly differ from the number that moved downslope. Fledglings moved greater distances and elevations than adults, but these movements were not upslope specific. Female fledglings showed greater changes in elevation and distance compared to male fledglings, but again, movements were not consistently upslope. The upslope shift in this population may be due to birds immigrating into the population and not from changes in individual nesting behavior.
Characterisation of the Behavioural Effects of a Thoracic Squeeze in Healthy Newborn Piglets
Authors: Holdsworth Sophia E. , Kells Nikki J. , Chidgey Kirsty L. , Vallée Emilie , Ward Neil , Mellor David J. , Beausoleil Ngaio J.
Published: August 2021

A thoracic squeeze has been observed to cause both healthy and low vigour neonatal foals to enter a ‘less-responsive state’, characterised by loss of posture, eye closure and cessation of movement, from which they rapidly recover to express normal healthy behaviours when the squeeze is released. To date, there have been no systematic studies characterising the responses of healthy neonates of other mammalian species to a thoracic squeeze. We describe the responses of healthy newborn piglets (n = 17) to a standardised application of the thoracic squeeze and evaluate the effect of the method of squeeze application on the response. Neonatal piglets were squeezed around the chest with either a soft fabric rope as has been used in foals (n = 8) or a novel purpose-made inflation cuff (n = 9). Both methods were effective at inducing a less-responsive behavioural state in all piglets, with neural reflexes reduced or absent in over half of them. The inflation cuff appeared to induce the less-responsive state faster than the rope, and more piglets squeezed with the cuff remained in this state for the full 10-min squeeze. These findings suggest that the behavioural response of foals to thoracic squeezing can be generalised to neonates of other precocial mammalian species. This initial study provides a foundation for further research using the inflation cuff to explore mechanisms underlying the thoracic squeeze and ways in which it may be applied whilst performing husbandry procedures.
Altered Proteomic Profile of Adipose Tissue-Derived Mesenchymal Stem Cell Exosomes from Cats with Severe Chronic Gingivostomatitis
Authors: Villatoro Antonio J. , Martín-Astorga María del Carmen , Alcoholado Cristina , Cárdenas Casimiro , Fariñas Fernando , Becerra José , Visser Rick
Published: August 2021

Feline chronic gingivostomatitis (FCGS) is a pathology with a complicated therapeutic approach and with a prevalence between 0.7 and 12%. Although the etiology of the disease is diverse, feline calicivirus infection is known to be a predisposing factor. To date, the available treatment helps in controlling the disease, but cannot always provide a cure, which leads to a high percentage of refractory animals. Mesenchymal stem cells (MSCs) play a pivotal role in the homeostasis and reparation of different tissues and have the ability to modulate the immune system responses. This ability is, in part, due to the capacity of exosomes to play a part in intercellular cell communication. However, the precise role of MSC-derived exosomes and their alterations in immunocompromised pathologies remains unknown, especially in veterinary patients. The goal of this work was to analyze the proteomic profile of feline adipose tissue-derived MSCs (fAd-MSCs) from calicivirus-positive FCGS patients, and to detect possible modifications of the exosomal cargo, to gain better knowledge of the disease’s etiopathogenesis. Using high-resolution mass spectrometry and functional enrichment analysis with Gene Ontology, exosomes isolated from the fAd-MSCs of five healthy cats and five calicivirus-positive FCGS patients, were pooled and compared. The results showed that the fAd-MSCs from cats suffering from FCGS not only had a higher exosome production, but also their exosomes showed significant alterations in their proteomic profile. Eight proteins were exclusively found in the exosomes from the FCGS group, and five proteins could only be found in the exosomes from the healthy cats. When comparing the exosomal cargo between the two groups, significant upregulation of 17 and downregulation of 13 proteins were detected in the FCGS group compared to the control group. These findings shed light on new perspectives on the roles of MSCs and their relation to this disease, which may help in identifying new therapeutic targets and selecting specific biomarkers.
Pharmacokinetic-Pharmacodynamic Profile, Bioavailability, and Withdrawal Time of Tylosin Tartrate Following a Single Intramuscular Administration in Olive Flounder (Paralichthys olivaceus)
Authors: Lee Ji-Hoon , Kim Ga Won , Kwon Mun-Gyeong , Seo Jung Soo
Published: August 2021

The objective of this study was to demonstrate the pharmacokinetic–pharmacodynamic profile, bioavailability, and withdrawal time of tylosin tartrate (TT) administered to olive flounder via intramuscular (IM, 10 or 20 mg/kg, n = 240) and intravascular (IV, 10 mg/kg, n = 90) injections. Serum concentrations of tylosin were determined using a validated liquid chromatography-tandem mass spectrometry method. According to the non-compartmental analysis, the bioavailability of TT was 87%. After the IV injection, the terminal half-life, total body clearance, volume of distribution, and mean residence time of TT were 21.07 h, 0.07 L/kg/h, 2.15 L/kg, and 16.39 h, respectively. Rapid absorption (Tmax 0.25 h), prolonged action (terminal half-life, 33.96 and 26.04 h; MRT, 43.66 and 33.09 h), and linear dose–response relationship (AUC0-inf, 123.55 and 246.05 µg/mL*h) were monitored following 10 and 20 mg/kg IM injection. The withdrawal time of TT from muscle (water temperature, 22 °C) was 9.84 days, rounded up to 10 days (220 degree days). Large Cmax/MIC90, AUC0-inf/MIC90, and T > MIC90 values were obtained for Streptococcus isolates and these PK/PD indices satisfied the criteria required for efficacy evaluation. This study lays a foundation for the optimal use of TT and provides valuable information for establishing therapeutic regimens.
Acid-Sensing Ion Channels in Zebrafish
Authors: Montalbano Giuseppe , Levanti Maria , Mhalhel Kamel , Abbate Francesco , Laurà Rosaria , Guerrera Maria Cristina , Aragona Marialuisa , Germanà Antonino
Published: August 2021

The ASICs, in mammals as in fish, control deviations from the physiological values of extracellular pH, and are involved in mechanoreception, nociception, or taste receptions. They are widely expressed in the central and peripheral nervous system. In this review, we summarized the data about the presence and localization of ASICs in different organs of zebrafish that represent one of the most used experimental models for the study of several diseases. In particular, we analyzed the data obtained by immunohistochemical and molecular biology techniques concerning the presence and expression of ASICs in the sensory organs, such as the olfactory rosette, lateral line, inner ear, taste buds, and in the gut and brain of zebrafish.
Optimization of a Protocol for the Cryopreservation of Sperm in Pellets for the Common Pheasant (Phasianus colchicus mongolicus)
Authors: Castillo Annelisse , Lenzi Carla , Pirone Andrea , Baglini Alessandro , Cerolini Silvia , Iaffaldano Nicolaia , Sartore Stefano , Russo Claudia , Schiavone Achille , Marzoni Fecia di Cossato Margherita
Published: August 2021

The sperm of each avian species and breed have unique characteristics that render them more or less susceptible to the freezing–thawing process; therefore, a suitable cryopreservation protocol that is specific for the sperm of each type of bird is needed. In this context, little information about the common pheasant’s sperm is available. Therefore, the aim of this study was to test different parameters at each step of the process of freezing into pellets and thawing to detect the least deleterious parameter settings. Sixteen different protocols were tested by studying two levels in each of the four steps (dilution, equilibration at 5 °C, final dimethylacetamide concentration, and dimethylacetamide equilibration time) comprising the freezing process. The pheasant sperm exhibited a high susceptibility to the damage caused by freezing into pellets; however, the survival of the sperm reached 29%, and the greatest recovered mobility was 22%. The mobility of the sperm was affected by the dilution and the dimethylacetamide concentration, and the viability of the sperm was affected by the equilibration at 5 °C and the dimethylacetamide equilibration. The protocols that caused the least damage to the pheasant sperm were found to be those with higher dilution rates, 10 min of equilibration at 5 °C, and 6% dimethylacetamide equilibrated for 1 or 5 min. In the present study, we individualise some applicable parameters for certain critical steps of the freezing–thawing process; however, further investigations are needed in order to improve upon and complete a suitable protocol for the cryopreservation and thawing of pheasant sperm.
Slaughter of Pregnant Cattle at an Austrian Abattoir: Prevalence and Gestational Age
Authors: Zitterer Ignaz , Paulsen Peter
Published: August 2021

The slaughter of pregnant cattle raises ethical–moral questions with regard to animal welfare, but also concerns of consumers because of higher levels of sex steroids in the meat from pregnant cattle. Since no data on the slaughter of pregnant cattle in Austria were available, we examined uteri of slaughtered female cattle in one Austrian mid-size abattoir. Sample size was calculated for an assumed prevalence of 2.5% (±1%; 95% confidence interval) of cows or heifers slaughtered in the last trimester of pregnancy and amounted to 870 cows and 744 heifers. 1633 female cattle of domestic origin were examined, most of them of dual-purpose type. Pregnancy was detected in 30/759 heifers and in 74/874 cows (an overall prevalence of 6.4%). The number of cattle in the last trimester of pregnancy was 16 to 26, depending on the evaluation scheme. We found no significant differences in percentages of pregnant cattle sent to slaughter for beef, dual-purpose and dairy breeds, although the latter group demonstrated the lowest percentage. Our results are comparable with those from previously conducted studies in other member states of the European Union. Measures to avoid sending pregnant cattle to slaughter should be implemented at farm-level.
Association of Particulate Matter from Cooking Oil Fumes with Heart Rate Variability and Oxidative Stress
Authors: Chan Chang-Chuan , Lin Lian-Yu , Lai Ching-Huang , Chuang Kai-Jen , Wu Ming-Tsang , Pan Chih-Hong
Published: August 2021

Many studies have reported various cardiovascular autonomic responses to ambient particulate matter (PM) pollution, but few have reported such responses to occupational PM exposures. Even fewer have demonstrated a relationship between PM pollution and oxidative stress in humans. This panel study evaluates the association between occupational exposure to PM in cooking oil fumes (COFs), and changes in both heart rate variability (HRV) and oxidative stress responses in 54 male Chinese cooks. Linear mixed-effects regression models were adopted to estimate the strength of the association between PM and HRV. Participants’ pre- and post-workshift urine samples were analyzed for 8-hydroxy-2′-deoxyguanosine (8-OHdG) and malondialdehyde (MDA). Exposure to PM in COFs from 15 min to 2 h were associated with a decrease in HRV and an increase in heart rate among cooks. The urinary 8-OHdG levels of cooks were significantly elevated after workshift exposure to COFs. The levels of PM2.5, PM1.0, and particulate benzo(a)pyrene in COFs were all positively correlated with cross-workshift urinary 8-OHdG levels. Furthermore, the levels of benzo(a)pyrene in COFs were positively correlated with cross-workshift urinary MDA levels. The effects of COFs on HRV were independent of cross-workshift urinary 8-OHdG levels. Exposure to COFs leads to disturbed autonomic function and an increased risk of oxidative DNA injury among cooks in Chinese restaurants.
Application of an Electrical Low Pressure Impactor (ELPI) for Residual Particle Measurement in an Epitaxial Growth Reactor
Authors: Lee Seungjae , Kim Dongbin , Cho Yujin , Kim Eunmi , Liu Pengzhan , Kwak Dong-Bin , Keum Seungho , Lim Hongkang , Kim Taesung
Published: August 2021

The purpose of this study was to determine the feasibility of using an electrical low pressure impactor (ELPI) for analyzing residual particles in a Si epitaxial growth process chamber and establish an application technique. Prior to experimental measurements, some preliminary works were conducted, including an inlet improvement of a cascade impactor, vacuum fitting fastening and flow rate adjustment, and a vacuum leak test. After that, residual particles in the process chamber were measured during N2 gas purge using an ELPI due to its advantages including the real-time measurement of particles and the ability to separate and collect particles by their diameters. In addition, ELPI could be used to obtain particle size distribution and see the distribution trend for both number and mass concentration. The results of the real-time analysis of the total particle count revealed that the concentration at the endpoint compared to that at the beginning of the measurement by decreased 36.9%. Scanning electron microscopy/energy-dispersive X-ray spectroscopy (SEM–EDS) analysis of collected particles was performed using two types of substrates: Al foil and a Si wafer. The results showed that most particles were Si particles, while few particles had Si and Cl components. ELPI has the clear advantages of real-time particle concentration measurement and simultaneous collection. Thus, we believe that it can be more actively used for particle measurement and analysis in the semiconductor industry, which has many critical micro/nanoparticle issues.