EXPERIMENTAL STUDY OF THE HEALING PROPERTIES OF BIOSEPTIN OINTMENT
Authors:   Novosibirsk State Medical University, Novosibirsk, Russia, Stavskiy Е.А. , Teplyakova Т.V. ,   State Scientific Center for Virology and Biotechnology “Vector” of Russian Federal State Agency for Health and Consumer Rights, Koltsovo, Novosibirsk region, Russia, Nadeev А.P. ,   Novosibirsk State Medical University, Novosibirsk, Russia, Malkova Е.М. ,   Novosibirsk Institute for Advanced Training of Healthcare Workers, Novosibirsk, Russia, Ilina T.N. ,   Medical and sanitary unit No. 163, FMBA of Russia, Novosibirsk, Russia, Ermachenko М.А. ,   Novosibirsk State Medical University, Novosibirsk, Russia, Stavskaya А.А. ,   Novosibirsk State Medical University, Novosibirsk, Russia, Shmidt А.D. ,   Novosibirsk State Medical University, Novosibirsk, Russia, Borzenko А.М. ,   Novosibirsk State Medical University, Novosibirsk, Russia, Zarubina Yu.D. ,   Novosibirsk State Medical University, Novosibirsk, Russia, Zhayvoron А.N. ,   Novosibirsk State Medical University, Novosibirsk, Russia, Shestak А.S. ,   Novosibirsk State Medical University, Novosibirsk, Russia
Published: December 2023

Introduction. Due to the acquisition of resistance to antibiotics by pyogenic microbiota, the problem of local (medicinal) treatment of wounds has become more acute. Aim. Evaluation of the medicinal properties of Bioseptin ointment in vivo on a model of a purulent incised skin wound of research animals. Materials and object of the research. Bioseptin ointment was developed by LLC “Research Center”. The active principle of the ointment is a microbial association: bacteria Baсillus subtilis strain VKPМ B-7048 and Bacillus licheniformis strain VKPМ B-7038, which has high antagonistic activity against a wide range of pathogenic and opportunistic microorganisms. As an auxiliary component of the ointment, a dense nutrient medium is taken, used for growing cultures of B. subtilis and B. licheniformis in the process of obtaining a specific batch of ointment. The ointment is a combination preparation for topical use. The wound-healing effectiveness of the ointment was assessed in two groups (20 mice each) of healthy non-inbred mice with a body weight of 16 and 20 g of ICR colony of both sexes from the nursery State Scientific Center for Virology and Biotechnology “Vector” of Russian Federal State Agency for Health and Consumer Rights. The control group was a group of mice treated with the reference ointment Iruksol (20 mice per group). A model of a purulent wound in animals of these three groups was obtained by infecting incised skin wounds with a mixture of bacterial cells P. aeruginosa and S. Aureus. Treatment of mice was continued until their incised skin wounds healed. Every day, mice in all groups were assessed for wound area, percentage reduction in wound area (WAR) according to L.N. Popova’s method, rate of wound contraction, motor activity, appetite, dynamics and nature of wound healing. Every 3 days, changes in body weight were assessed, laboratory blood tests and histomorphological examination of the incised skin wound were performed. To obtain tissue samples, animals, according to the observation period were removed from the experiment by euthanasia using the method of cervical dislocation. Tissue samples excised from the wound and blood samples were examined on days 3, 6, 9, and 12 after skin wound formation. Samples for light microscopy were prepared and stained according to the methods. Viewing of preparations and microphotography was carried out on a Jenaval light microscope (Carl Zeiss, Jena, Germany). All data were processed statistically using Microsoft Excel and STATISTICA. Results and discussion. It was shown that almost until the end of treatment of purulent wounds in all groups of experimental animals with the control ointment Iruksol and Bioseptin ointment, negative dynamics of body weight was observed due to the expressed toxic effect of the purulent wound on the body of the research animals. The results of a blood test for hematological and biochemical parameters in the body of research animals of all three groups revealed changes characteristic of the development of a local infectious process and intoxication. Evidence was established of faster healing of purulent wounds in animals of groups 1 and 2 treated with Bioseptin and Iruksol ointments, respectively, compared to animals of group 3 (with a severe superinfected purulent wound). Moreover, complete visually observed wound healing in group 2 occurred in less than 9 days, in control group 1 and experimental group 3 on the 12th day of observation. Histomorphological study demonstrated the restoration of the structure of the skin in the wound as an organ with all its derivatives already on the 9th day in groups 1-2; in group 3 with a burdened purulent wound, complete restoration of the epidermal defect was not noted. Conclusion. Bioseptin ointment is promising as a drug for effective topical use in the treatment of infected wounds; the ointment has a significant antimicrobial effect. The microbial association (Bacillus subtilis strain ВКПМ B-7048 and Bacillus licheniformis strain ВКПМ B-7038) which is part of the Bioseptin ointment, promotes the cleansing of wounds, their granulation and faster healing compared to the control ointment Iruksol. The use of Bioseptin ointment for the treatment of complicated purulent wounds has been shown to be promising.