bluuebunny/crossref_metadata_2025 huggingface dataset:
Shielding of Photosynthetic Apparatus by Consortia of Bacterial Endophytes in Tomato Plants Suffering From Fusarium Wilt
Authors: Chaturvedi Himani , Singh Bhupendra , Jajoo Anjana , Prakash Anil
Published: May 2022

Fusarium oxysporum is one of the most damaging plant pathogens causing Fusarium wilt in many plants leading to serious economic loss. The fungus colonizes the xylem, which leads to resistance in water flow in the plant thereby affecting the rate of photosynthesis. The present study focuses on the selection of bacterial endophytes isolated from tomato plants and evaluating their potential to antagonize Fusarium oxysporum in tomato in vivo. The results obtained indicated that two endophytic isolates, namely Pseudomonas fluorescens BUMD5 and Bacillus velezensis BUMD9, could act as efficient biocontrol agents (BCAs) as they inhibited the growth of pathogen by 67.2 and 69.1%, respectively, in vitro. Both the isolates were found to produce hydrolytic enzymes chitinase and protease. They also produced siderophore and hydrogen cyanide (HCN). The consortia of both the isolates significantly reduced the infection percentage by about 67% and a 3-fold decrease in disease severity was observed as compared to pathogen control. The treatment of infected plants with these potent isolates was also beneficial in improving the overall photosynthetic performance index (PI). Thus, plants treated with consortia of these isolates exhibited better overall plant growth despite being infected by the pathogen.
Aerodynamic Performance of a Dragonfly-Inspired Tandem Wing System for a Biomimetic Micro Air Vehicle
Authors: Salami Erfan , Montazer Elham , Ward Thomas A , Nik Ghazali Nik Nazri , Anjum Badruddin Irfan
Published: May 2022

The flying agility demonstrated by dragonflies is accomplished by means of complex aerodynamic forces produced by flapping their four wings arranged in a tandem configuration. The current study presents a novel tandem flapping wing mechanism for a biomimetic air vehicle that was designed and manufactured to experimentally investigate the aerodynamic forces. By optimizing the configuration and using spatial network analysis, it is shown that the designed structure can flap the wings in a linear up–down stroke motion and is capable of maintaining good consistency and aerodynamic performance. Such a mechanism could be used in a future biomimetic micro air vehicle (BMAV) design. The mechanism uses an electromagnetic actuator to flap the wings with a variable beat frequency (30–210 Hz) at various angles of attack (−10°–20°). The results show that the tandem wings generate approximately 50% higher lift than the forewing or hindwing pairs acting alone. Tandem wings also improve stability, which could potentially allow hovering.
Biological Effects of Maslinic Acid on Human Epithelial Cells Used in Tissue Engineering
Authors: Ortiz-Arrabal Olimpia , Chato-Astrain Jesús , Crespo Pascual Vicente , Garzón Ingrid , Mesa-García María Dolores , Alaminos Miguel , Gómez-Llorente Carolina
Published: May 2022

In the present work, we evaluated the potential of maslinic acid (MA) to improve currently available keratinocyte culture methods for use in skin tissue engineering. Results showed that MA can increase cell proliferation and WST-1 activity of human keratinocytes after 24, 48, and 72 h, especially at the concentration of 5 μg/ml, without affecting cell viability. This effect was associated to a significant increase of KI-67 protein expression and upregulation of several genes associated to cell proliferation (PCNA) and differentiation (cytokeratins, intercellular junctions and basement membrane related genes). When human keratinocytes were isolated from skin biopsies, we found that MA at the concentration of 5 μg/ml significantly increased the efficiency of the explant and the cell dissociation methods. These results revealed the positive effects of MA to optimize human keratinocyte culture protocols for use in skin tissue engineering.
Beyond Genetics: The Role of Metabolism in Photoreceptor Survival, Development and Repair
Authors: Hanna Joseph , David Luke Ajay , Touahri Yacine , Fleming Taylor , Screaton Robert A. , Schuurmans Carol
Published: May 2022

Vision commences in the retina with rod and cone photoreceptors that detect and convert light to electrical signals. The irreversible loss of photoreceptors due to neurodegenerative disease leads to visual impairment and blindness. Interventions now in development include transplanting photoreceptors, committed photoreceptor precursors, or retinal pigment epithelial (RPE) cells, with the latter protecting photoreceptors from dying. However, introducing exogenous human cells in a clinical setting faces both regulatory and supply chain hurdles. Recent work has shown that abnormalities in central cell metabolism pathways are an underlying feature of most neurodegenerative disorders, including those in the retina. Reversal of key metabolic alterations to drive retinal repair thus represents a novel strategy to treat vision loss based on cell regeneration. Here, we review the connection between photoreceptor degeneration and alterations in cell metabolism, along with new insights into how metabolic reprogramming drives both retinal development and repair following damage. The potential impact of metabolic reprogramming on retinal regeneration is also discussed, specifically in the context of how metabolic switches drive both retinal development and the activation of retinal glial cells known as Müller glia. Müller glia display latent regenerative properties in teleost fish, however, their capacity to regenerate new photoreceptors has been lost in mammals. Thus, re-activating the regenerative properties of Müller glia in mammals represents an exciting new area that integrates research into developmental cues, central metabolism, disease mechanisms, and glial cell biology. In addition, we discuss this work in relation to the latest insights gleaned from other tissues (brain, muscle) and regenerative species (zebrafish).
Magnetic Hyperbranched Molecular Materials for Treatment of Oily Sewage Containing Polymer in Oilfield Compound Flooding
Authors: Qiao Sanyuan , Liu Qingwang , Fan Zhenzhong , Tong Qilei , Cai Li , Fu Yuanfeng
Published: May 2022

With the continuous improvement in oilfield development and the application of tertiary oil recovery technology, the water content of oilfield-produced fluids has gradually increased, and a large number of oilfield sewage with complex components has also been produced after oil–water separation, and effective treatment is urgently needed. ASP flooding sewage contains alkali, various surfactants, polymers, microemulsion oil droplets, and solid impurities, which are difficult to be effectively treated by traditional water treatment agents and methods. In this study, aminopropyl triethoxysilane (APTES) was used to modify the nano-Fe3O4 coated with tetraethyl silicate (TEOS). The product was used as the ferromagnetic nano-core for the iterative reaction of Michael addition and ester amidation to synthesize a magnetic hyperbranched polyamide amine, and its performance in the treatment of ASP flooding wastewater was evaluated experimentally. For the preparation of APTES-modified Fe3O4@SiO2 (FOSN) product, TEOS was coated over Fe3O4 in an ethanol aqueous solution environment and then APTES was added dropwise. The first-generation branched product (1-FSMN) was obtained by the reaction of FOSN and methyl acrylate graft product (FOSN-M) with ethylenediamine, and the highest yield was 93.7%. The highest yield of the second-generation branched product (2-FSMN) was 91.6%. In this study, a composite flooding wastewater sample from a block in the Bohai oilfield was taken. The suspended solids content was 143 mg/L, the oil content was 921.09 mg/L, the turbidity was 135 NTU, and the zeta potential was −47 mV. The third-generation hyperbranched polymer (3-FSMN) and its quaternary ammonium salt (3-FSMN-Q) performed best in the appropriate dosage range, with the highest oil removal rate of 97%, suspended solid removal rate of 90.3%, turbidity reduction rate of 86.6% and zeta potential reduction rate of 88%. For 3-FSMN and its quaternary ammonium salt, the gravity/magnetic PAC compound treatment experiment was carried out. In the settlement time of only 5 min, 3-FSMN/PAC and 3-FSMN-Q/PAC can achieve the maximum oil removal rate of 87.1% and suspended solids removal rate of 87.3% for polymer containing wastewater from ASP flooding, and 86.3 and 86.0% for 120 mg/L. Its treatment capacity was much better than that of common treatment agent combination (CPAM/PAC).
“Cut and Paste” Processes in the Search of Bioactive Products: One-Pot, Metal-free O-Radical Scission-Oxidation-Addition of C, N or P-Nucleophiles
Authors: Porras Marina , Hernández Dácil , González Concepción C. , Boto Alicia
Published: May 2022

Hypervalent iodine reagents have been applied in many metal-free, efficient synthesis of natural products and other bioactive compounds. In particular, treatment of alcohols, acetals and acids with hypervalent iodine reagents and iodine results in O-radicals that can undergo a β-scission reaction. Under these oxidative conditions, derivatives of amino acids, peptides or carbohydrates are converted into cationic intermediates, which can subsequently undergo inter- or intramolecular addition of nucleophiles. Most reported papers describe the addition of oxygen nucleophiles, but this review is focused on the addition of carbon, nitrogen and phosphorous nucleophiles. The resulting products (nucleoside and alkaloid analogs, unnatural amino acids, site-selectively modified peptides) are valuable intermediates or analogs of bioactive compounds.
Rational Engineering of Anode Current Collector for Dendrite-Free Lithium Deposition: Strategy, Application, and Perspective
Authors: Li Nanrui , Jia Tianqi , Liu Yanru , Huang Shifei , Kang Feiyu , Cao Yidan
Published: May 2022

Lithium metal anodes have attracted extensive attention due to their high theoretical capacity and low redox potential. However, low Coulombic efficiency, serious parasitic reaction, large volume change, and dendrite growth during cycling have hindered their practical application. The engineering of an anode current collector provides important advances to solve these problems, eliminate excess lithium usage, and substantially increase the energy density. In this review, we summarize the engineering strategies of an anode current collector with emphasis on different methods and applications in lithium metal-based systems. Finally, the perspectives and challenges of current collector engineering for lithium metal anode are discussed.
The Efficacy and Safety of the Combined Therapy of Sodium-Glucose Co-Transporter-2 Inhibitors and Angiotensin Receptor-Neprilysin Inhibitor in Patients With Heart Failure With Reduced Ejection Fraction: A Meta-Analysis of the EMPEROR-Reduced and DAPA-HF Sub-Analysis
Authors: Lin Yanxia , Zhang Huanrui , Zhao Shijie , Chen Ling , Li Jinyang , Wang Xiaoou , Tian Wen
Published: May 2022

Background

Both sodium-glucose co-transporter-2 (SGLT-2) inhibitors and angiotensin receptor-neprilysin inhibitor (ARNI) were recommended to treat heart failure with reduced ejection fraction (HFrEF). However, no trial was conducted to assess the efficacy and safety of the combined therapy of SGLT-2 inhibitors and ARNI in patients with HFrEF.

Methods

We performed a meta-analysis of the prespecified subgroups from DAPA-HF and EMPEROR-Reduced trials. The primary endpoint was the composite risk of cardiovascular death or hospitalization for heart failure. The risk of cardiovascular death, all-cause death, a composite of serious adverse renal outcomes, and volume depletion were also estimated.

Results

The risk of the composite of cardiovascular death or hospitalization for heart failure was reduced in combined therapy of SGLT-2 inhibitors and ARNI, compared with ARNI monotherapy (RR.68, 95% CI.53 to.85, P = 0.001). When compared with SGLT-2 inhibitors monotherapy, the events of cardiovascular death (RR.64, 95% CI.46 to 0.87, P = 0.005) and all-cause death (RR.72, 95% CI.55 to.94, P = 0.01) were significantly less in combined therapy, accompanied by elevated incidence of volume depletion (RR 1.55, 95% CI 1.22 to 1.96, P = 0.0003).

Conclusion

Combined therapy has additional benefits over monotherapy in patients with HFrEF, however, it is accompanied by a possibly higher risk of volume depletion.
Cardiac and Vascular Remodeling After 6 Months of Therapy With Sacubitril/Valsartan: Mechanistic Insights From Advanced Echocardiographic Analysis
Authors: Monosilio Sara , Filomena Domenico , Luongo Federico , Sannino Michele , Cimino Sara , Neccia Matteo , Mariani Marco Valerio , Birtolo Lucia Ilaria , Benedetti Giulia , Tonti Giovanni , Pedrizzetti Gianni , Vizza Carmine Dario , Maestrini Viviana , Agati Luciano
Published: May 2022

Background

Effects of Sacubitril/Valsartan (S/V) on left ventricular (LV) mechanics and ventricular-arterial coupling in patients with heart failure with reduced ejection fraction (HFrEF) are not completely understood. The aim of this study was to evaluate both cardiac and vascular remodeling in a group of HFrEF patients undergoing S/V therapy.

Methods

Fifty HFrEF patients eligible to start a therapy with S/V were enrolled. Echocardiographic evaluation was performed at baseline and after 6 months of follow-up (FU). Beside standard evaluation, including global longitudinal strain (GLS), estimated hemodynamic forces (HDFs) and non-invasive pressure-volume curves (PV loop) were assessed using dedicated softwares. HDFs were evaluated over the entire cardiac cycle, in systole and diastole, both in apex to base (A-B) and latero-septal (L-S) directions. The distribution of LV HDFs was evaluated by L-S over A-B HDFs ratio (L-S/A-B HDFs ratio). Parameters derived from estimated PV loop curves were left ventricular end-systolic elastance (Ees), arterial elastance (Ea), and ventricular-arterial coupling (VAC).

Results

At 6 months of FU indexed left ventricular end-diastolic and end-systolic volumes decreased (EDVi: 101 ± 28 mL vs. 86 ± 30 mL, p < 0.001; ESVi: 72 ± 23 mL vs. 55 ± 24 mL, p < 0.001), ejection fraction and GLS significantly improved (EF: 29 ± 6% vs. 37 ± 7%, p < 0.001; GLS: −9 ± 3% vs. −13 ± 4%, p < 0.001). A reduction of Ea (2.11 ± 0.91 mmHg/mL vs. 1.72 ± 0.44 mmHg/mL, p = 0.008) and an improvement of Ees (1.01 ± 0.37 mmHg/mL vs. 1.35 ± 0.6 mmHg/mL, p < 0.001) and VAC (2.3 ± 1.1 vs. 1.5 ± 0.7, p < 0.001) were observed. Re-alignment of HDFs occurred, with a reduction of diastolic L-S/A-B HDFs ratio [23 (20–35)% vs. 20 (11–28) %, p < 0.001].

Conclusion

S/V therapy leads to a complex phenomenon of reverse remodeling involving increased myocardial contractility, HDFs distribution improvement, and afterload reduction.
Discordance of Apolipoprotein B, Non-HDL-Cholesterol, and LDL-Cholesterol Predicts Risk of Increased Arterial Stiffness and Elevated Carotid Intima-Media Thickness in Middle-Aged and Elderly Chinese Adults
Authors: Jia Xiaojing , Qi Yan , Zheng Ruizhi , Lin Lin , Hu Chunyan , Zhu Yuanyue , Cao Qiuyu , Wu Xueyan , Qi Hongyan , Wei Ran , Zhang Yi , Xu Min , Xu Yu , Wang Tiange , Zhao Zhiyun , Chen Yuhong , Li Mian , Wang Weiqing , Bi Yufang , Lu Jieli
Published: May 2022

Background

Apolipoprotein B (apoB) and non-high-density lipoprotein cholesterol (non-HDL-C) have been shown to predict cardiovascular disease (CVD) even in the case of low levels of low-density lipoprotein cholesterol (LDL-C). We aimed to investigate whether the discordance between LDL-C and apoB or non-HDL-C was associated with arterial stiffness and elevated carotid intima-media thickness (CIMT) in middle-aged and elderly adults.

Methods

A total of 5,279 Chinese adults free of CVD at baseline were included and followed with a mean follow-up of 4.3 years. Arterial stiffness was measured by brachial-ankle pulse wave velocity (baPWV) and pulse pressure (PP). The associations of apoB, non-HDL-C, and LDL-C with arterial stiffness or elevated CIMT were examined with logistic regression models using either continuous scales by restricted cubic splines or categories of concordant and discordant values defined by medians.

Results

High apoB but not LDL-C was associated with elevated baPWV or PP. High apoB, non-HDL-C, and LDL-C were all associated with elevated CIMT (p < 0.05). Individuals with low levels of LDL-C and discordantly high apoB or non-HDL-C compared to those with concordantly low apoB or non-HDL-C demonstrated higher risks of elevated baPWV [ORs (95% CI) of 1.40 (1.03–1.91) and 1.56 (1.12–2.18), respectively] and elevated PP [ORs (95% CI) of 1.61 (1.19–2.18) and 1.55 (1.12–2.15), respectively]. While, discordant high LDL-C with low apoB was associated with an increased risk of elevated CIMT (OR, 1.74; 95% CI, 1.13–2.69).

Conclusion

Discordance analysis revealed that elevated apoB or non-HDL-C was a better predictor of risk of arterial stiffness, whereas LDL-C for elevated CIMT.
New Perspectives on Glacial Geomorphology in Earth’s Deep Time Record
Authors: Le Heron D. P. , Busfield M. E. , Chen X. , Corkeron M. , Davies B. J. , Dietrich P. , Ghienne J-F. , Kettler C. , Scharfenberg L. , Vandyk T. M. , Wohlschlägl R.
Published: May 2022

The deep time (pre-Quaternary) glacial record is an important means to understand the growth, development, and recession of the global cryosphere on very long timescales (106–108Myr). Sedimentological description and interpretation of outcrops has traditionally played an important role. Whilst such data remain vital, new insights are now possible thanks to freely accessible aerial and satellite imagery, the widespread availability and affordability of Uncrewed Aerial Vehicles, and accessibility to 3D rendering software. In this paper, we showcase examples of glaciated landscapes from the Cryogenian, Ediacaran, Late Ordovician and Late Carboniferous where this approach is revolutionizing our understanding of deep time glaciation. Although some problems cannot be overcome (erosion or dissolution of the evidence), robust interpretations in terms of the evolving subglacial environment can be made. Citing examples from Australia (Cryogenian), China (Ediacaran), North and South Africa (Late Ordovician, Late Carboniferous), and Namibia (Late Carboniferous), we illustrate how the power of glacial geomorphology can be harnessed to interpret Earth’s ancient glacial record.
An Examination of the Paths of Successful Diverse STEM Faculty: Insight for Programming
Authors: Murphrey Theresa Pesl , Carter Carmen R. , Regisford E. Gloria C. , Carson Laura E. , Butler-Purry Karen , Carter-Sowell Adrienne R. , Ganesan Asha , Richburg Audra
Published: May 2022

Understanding the experiences of successful diverse science, technology, engineering, and math (STEM) faculty can facilitate the development of programming that counteracts barriers and weaknesses from multiple angles. The challenges that students and professionals report can be broadly identified as either identity-based or institutional. The lack of diversity in STEM fields in academia can result in narrow viewpoints, limited student diversity, and missed opportunities to address today’s societal challenges. It is clear that we must consider programming that has positively impacted successful STEM faculty in academia in order to create effective programming to recruit and retain future diverse STEM faculty. Our phenomenological study sought to add to the literature related to the role that socialization plays in preparing individuals for success in faculty roles by conducting in-depth interviews with early-career STEM faculty members in under-represented groups. The phenomena under investigation were experiences leading to early-career STEM faculty members’ successful career pathways. Seven early-career STEM faculty from multiple institutions described unique paths to their current faculty position with some commonalities, including participation in undergraduate or postdoc research and having some industry experience. The suggestions, advice, and guidance offered by the participants fell into categories that, while mirrored in the literature, serve as useful markers for administrators developing programming. We organized our findings using the conceptual framework of socialization and the associated competencies for our context. As we strive to encourage and build diverse representation in populations of STEM academicians, these collective findings are invaluable. Findings confirm that programming directly impacts the success of early-career STEM faculty, and it is the success of these individuals that will enable diversity and inclusion to expand in STEM. Programs, interventions, and additional efforts for graduate students can also benefit from close examination of these experiences.
The Research Competence: Acquisition and Development Among Undergraduates in Education Sciences
Authors: Ciraso-Calí Anna , Martínez-Fernández J. Reinaldo , París-Mañas Georgina , Sánchez-Martí Angelina , García-Ravidá Laura B.
Published: May 2022

Known as one of the competences of the 21st century, research competence can help students navigate through the complexities of a continuously shifting world. This study aims to analyze the acquisition and development of this competence in a sample of 154 undergraduate students of two bachelor’s degrees in Education Sciences (Social Education and Pedagogy) of the Universitat Autònoma de Barcelona (Spain). We conducted a three-phase study, in which (1) the learning outcomes related to research competences declared in the syllabi were identified and mapped through a content analysis of each syllabus; (2) students’ perceptions about the development of these learning outcomes were gathered through a questionnaire; and (3) guidelines to foster research competences among these undergraduates were explored by a Delphi panel technique. The results show that communicative skills and state-of-art reviewing skills are the least present across the courses of both degrees. The design of research competency acquisition across courses is uneven and does not seem clearly articulated. The students’ perception is consistent with the shortcomings, or disarticulation, observed in the curriculum analysis. They consider that the most poorly acquired competencies are the state-of-the-art reviewing, content knowledge, and communicative skills. Apparently, more emphasis is given to reflective thinking and communicative skills; but still, it is necessary to strengthen the acquisition of scientific content, the search for trustworthy information. These results were discussed with two panels of experts from which guidelines were defined to improve the acquisition, development, and evaluation of the research competence through university training in this field.
Body Composition in Acromegaly According to Disease Activity – Performance of Dual X-Ray Absorptiometry and Multifrequency Bioelectrical Impedance Analysis
Authors: Lopes Aline Alves , Albuquerque Luciano , Fontes Mayara , Rego Daniella , Bandeira Francisco
Published: May 2022

Introduction

the present study aims to evaluate body composition and its relationship with hormonal control in acromegaly, also comparing the performance of Bioelectrical impedance analysis (BIA), a more accessible method, with dual X-ray absorptiometry (DXA), technology frequently used in current studies.

Methods

we studied 28 patients (78% female) of whom 13 with active disease, mean age was 52.11 ± 12.53 years; 64% had high blood pressure (HBP) and 50% had type 2 diabetes (T2D).

Results

Although patients with controlled disease had lower serum GH (1.2 ± 1.68µg/L vs 6.61 ± 6.40µg/L, p=0.001) and IGF-1 (158.89 ± 54.53ng/mL and 503.31 ± 253.25ng/mL, p<0.001), they did not differ in body composition: percentage of fat mass: 36.13 ± 11.84% vs 37.73 ± 8.76%, p=0.691 for BIA and 37.10 ± 10.21% vs 37.89 ± 7.32%, p=0.819 for DXA; muscle mass parameters, BIA: FFMI 18.68 ± 2.38kg/m2vs 19.14 ± 1.59kg/m2, p=0.560; SMI 10.17 ± 1.39kg/m2vs 10.53 ± 1.01kg/m2, p=0.438; DXA: Baumgartner 7.99 ± 1.43kg/m2vs 8.02 ± 1.24kg/m2, p=0.947, respectively for controlled and active disease. Patients with controlled acromegaly had lower fasting glucose (110.33 ± 55.48mg/dL vs 129.77 ± 40.17mg/dL, p=0.033) and were less likely to have persistent T2D (28.6 vs 71.4%, p=0.008) and HBP (38.9 vs 61.1%, p=0.049). There were strong positive correlations between BIA and DXA for fat mass (r=0.929, p<0.001) and muscle mass parameters: SMI X Baumgartner: r=0.890, p<0.001; and FFMI X Baumgartner: r=0.868, p<0.001.

Conclusion

our data showed similar results in body composition assessment by BIA and DXA, with good correlation between the methods, regardless of the hormonal status of acromegaly. Furthermore, in patients with adequate hormonal control, there was preservation of muscle mass and a lower prevalence of metabolic comorbidities, such as T2D and HBP.
Characteristics and Evolution of China’s Carbon Emission Reduction Measures: Leading Towards Environmental Sustainability
Authors: Zhang Wenyu , Zhang Changyou , Wei Songbo , Zhang Qiang , Rehman Abdul , Shah Adeel
Published: May 2022

The increasing concentration of carbon emission (CE) in the atmosphere, which causes climate change (CC), has become a priority at the global level. The Chinese govt has enacted a series of CE reduction policies and regulations during the recent decades, which have effectively encouraged the country’s green and sustainable growth. For sustainable development and subsequent policy design, it is critical to investigate the features and evolution of CE reduction programs. First, we collected China’s national-level CE reduction policy texts from 2007 to 2021 as research objects. Second, using content analysis and NVivo software, a three-dimensional analytical framework of “Policy tools--Development stages--Policy implementation objects” is developed and investigated based on the theory of policy tools. Finally, the CE reduction policy language is coded line by line using empirical analysis, yielding 725 reference points. The results show that 1) The results show that: 1) in the evolution of CE reduction policy, environmental policy tools are the most used, accounting for 51.17%, and the use of supply-based and demand-based policy tools account for 32.28 and 16.55%, respectively; 2) demand-based policy tools need to be strengthened, and the structure of supply-based and environmental policy tools needs to be adjusted; 3) the govt’s coordination with research institutions and the public is not sufficient The implementation of policy instruments is insufficient; 4) China’s CE reduction policy is gradually developing from a macroscopic single subject policy to a diversified and specific policy with specialized content. Therefore, we recommend optimizing the policy structure in the light of social characteristics and strengthening the government’s guiding role in China’s CE reduction efforts. It is also recommended to increase financial instruments and enhance the binding force of laws and regulations.
Skull Sutures and Cranial Mechanics in the Permian Reptile Captorhinus aguti and the Evolution of the Temporal Region in Early Amniotes
Authors: Abel Pascal , Pommery Yannick , Ford David Paul , Koyabu Daisuke , Werneburg Ingmar
Published: May 2022

While most early limbed vertebrates possessed a fully-roofed dermatocranium in their temporal skull region, temporal fenestrae and excavations evolved independently at least twice in the earliest amniotes, with several different variations in shape and position of the openings. Yet, the specific drivers behind this evolution have been only barely understood. It has been mostly explained by adaptations of the feeding apparatus as a response to new functional demands in the terrestrial realm, including a rearrangement of the jaw musculature as well as changes in strain distribution. Temporal fenestrae have been retained in most extant amniotes but have also been lost again, notably in turtles. However, even turtles do not represent an optimal analog for the condition in the ancestral amniote, highlighting the necessity to examine Paleozoic fossil material. Here, we describe in detail the sutures in the dermatocranium of the Permian reptileCaptorhinus aguti(Amniota, Captorhinidae) to illustrate bone integrity in an early non-fenestrated amniote skull. We reconstruct the jaw adductor musculature and discuss its relation to intracranial articulations and bone flexibility within the temporal region. Lastly, we examine whether the reconstructed cranial mechanics inC. aguticould be treated as a model for the ancestor of fenestrated amniotes. We show thatC. agutilikely exhibited a reduced loading in the areas at the intersection of jugal, squamosal, and postorbital, as well as at the contact between parietal and postorbital. We argue that these “weak” areas are prone for the development of temporal openings and may be treated as the possible precursors for infratemporal and supratemporal fenestrae in early amniotes. These findings provide a good basis for future studies on other non-fenestrated taxa close to the amniote base, for example diadectomorphs or other non-diapsid reptiles.
Dynamic Routing of Heterogeneous Users After Traffic Disruptions Under a Mixed Information Framework
Authors: Folsom Larkin , Park Hyoshin , Pandey Venktesh
Published: May 2022

This research focuses on reducing traffic congestion using the competing strategies between informed and uninformed drivers. Under a mixed information framework, a navigation app provides within-day route suggestions to informed drivers using predicted information about the time-varying route habits of uninformed drivers. The informed users detour from initially proposed routes to minimize network congestion after traffic disruptions, pushing the system toward optimal equilibrium, while uninformed drivers make day-to-day decisions which push the system toward user equilibrium. Simulations considering varying fractions of informed drivers show that congestion is reduced during abrupt phase transition before reaching equilibrium by approximately 59.2% when 20% of drivers are informed, and is nearly eliminated when 80% of drivers are informed, which could be achieved through connected vehicle technologies. Shared memory multi-core parallelization improved the computational efficiency.
Unravelling the Role of Epigenetic Modifications in Development and Reproduction of Angiosperms: A Critical Appraisal
Authors: Kumari Priyanka , Khan Sajid , Wani Ishfaq Ahmad , Gupta Renu , Verma Susheel , Alam Pravej , Alaklabi Abdullah
Published: May 2022

Epigenetics are the heritable changes in gene expression patterns which occur without altering DNA sequence. These changes are reversible and do not change the sequence of the DNA but can alter the way in which the DNA sequences are read. Epigenetic modifications are induced by DNA methylation, histone modification, and RNA-mediated mechanisms which alter the gene expression, primarily at the transcriptional level. Such alterations do control genome activity through transcriptional silencing of transposable elements thereby contributing toward genome stability. Plants being sessile in nature are highly susceptible to the extremes of changing environmental conditions. This increases the likelihood of epigenetic modifications within the composite network of genes that affect the developmental changes of a plant species. Genetic and epigenetic reprogramming enhances the growth and development, imparts phenotypic plasticity, and also ensures flowering under stress conditions without changing the genotype for several generations. Epigenetic modifications hold an immense significance during the development of male and female gametophytes, fertilization, embryogenesis, fruit formation, and seed germination. In this review, we focus on the mechanism of epigenetic modifications and their dynamic role in maintaining the genomic integrity during plant development and reproduction.
Systems-Level Mapping of Cancer Testis Antigen 1b/a to Sarcoma Pathways Identifies Activated Ran Binding-2 E3 SUMO-Protein Ligase and Transducin-Like Enhancer Protein 1
Authors: Papanikolaou Nikolaos A. , Hytiroglou Prodromos , Pantelidou Pavlina , Papavassiliou Athanasios G. , Old Lloyd L.
Published: May 2022

Here we describe the identification of genes and their encoded proteins that are expressed in advanced grade tumors by reconstruction of a sarcoma cancer testis gene 1b/a (catg1b/a) network. CTAG1B/A is an ortholog of the yeast/Drosophila transcription factor Pcc1p, and a member of the KEOPS transcription complex. It has been implicated in telomere maintenance and transcriptional regulation through association with chromatin remodeling factors and is only expressed during adult testis germ cell differentiation. Ctag1b/a is re-activated in synovial sarcomas and myxoid liposarcomas but not in differentiated liposarcomas. We mapped CTAG1B/A protein to sarcoma transcription pathways with gene set expression analysis (GSEA) and using independent samples, we immunohistochemically identified expression of at least two network neighbors, RANBP2, and TLE1, thus validating our approach. This work demonstrates that mapping unknown genes to functional pathways by network re-construction is a powerful tool that can be used to identify candidate oncoproteins.
Identifying Quantitative Trait Loci for Thousand Grain Weight in Eggplant by Genome Re-Sequencing Analysis
Authors: Qian Zongwei , Ji Yanhai , Li Ranhong , Lanteri Sergio , Chen Haili , Li Longfei , Jia Zhiyang , Cui Yanling
Published: May 2022

Eggplant (Solanum melongena L.; 2n = 24) is one of the most important Solanaceae vegetables and is primarily cultivated in China (approximately 42% of world production) and India (approximately 39%). Thousand-grain weight (TGW) is an important trait that affects eggplant breeding cost and variety promotion. This trait is controlled by quantitative trait loci (QTLs); however, no quantitative trait loci (QTL) has been reported for TGW in eggplant so far, and its potential genetic basis remain unclear. In this study, two eggplant lines, 17C01 (P1, wild resource, small seed) and 17C02 (P2, cultivar, large seed), were crossed to develop F1, F2 (308 lines), BC1P1 (44 lines), and BC1P2 (44 lines) populations for quantitative trait association analysis. The TGWs of P1, P2 and F1 were determined as 3.00, 3.98 and 3.77 g, respectively. The PG-ADI (polygene-controlled additive-dominance-epistasis) genetic model was identified as the optimal model for TGW and the polygene heritability value in the F2 generation was as high as 80.87%. A high-quality genetic linkage bin map was constructed with resequencing analysis. The map contained 3,918 recombination bins on 12 chromosomes, and the total length was 1,384.62 cM. A major QTL (named as TGW9.1) located on chromosome 9 was identified to be strongly associated with eggplant TGW, with a phenotypic variance explanation of 20.51%. A total of 45 annotated genes were identified in the genetic region of TGW9.1. Based on the annotation of Eggplant genome V3 and orthologous genes in Arabidopsis thaliana, one candidate gene SMEL_009g329850 (SmGTS1, encoding a putative ubiquitin ligase) contains 4 SNPs and 2 Indels consecutive intron mutations in the flank of the same exon in P1. SmGTS1 displayed significantly higher expression in P1 and was selected as a potential candidate gene controlling TGW in eggplant. The present results contribute to shed light on the genetic basis of the traits exploitable in future eggplant marker-assisted selection (MAS) breeding.
Genetic and Epigenetic Mechanisms Deregulate the CRL2pVHL Complex in Hepatocellular Carcinoma
Authors: Minatel Brenda C. , Cohn David E. , Pewarchuk Michelle E. , Barros-Filho Mateus C. , Sage Adam P. , Stewart Greg L. , Marshall Erin A. , Telkar Nikita , Martinez Victor D. , Reis Patricia P. , Robinson Wendy P. , Lam Wan L.
Published: May 2022

Dysregulation of ubiquitin-proteasome pathway genes through copy number alteration, promoter hypomethylation, and miRNA deregulation is involved in cancer development and progression. Further characterizing alterations in these genes may uncover novel drug targets across a range of diseases in which druggable alterations are uncommon, including hepatocellular carcinoma (HCC). We analyzed 377 HCC and 59 adjacent non-malignant liver tissue samples, focusing on alterations to component genes of the widely studied CRL2pVHL E3 ubiquitin ligase complex. mRNA upregulation of the component genes was common, and was correlated with DNA hypomethylation and copy number increase, but many tumours displayed overexpression that was not explained by either mechanism. Interestingly, we found 66 miRNAs, including 39 previously unannotated miRNAs, that were downregulated in HCC and predicted to target one or more CRL2pVHL components. Several miRNAs, including hsa-miR-101-3p and hsa-miR-139-5p, were negatively correlated with multiple component genes, suggesting that miRNA deregulation may contribute to CRL2pVHL overexpression. Combining miRNA and mRNA expression, DNA copy number, and methylation status into one multidimensional survival analysis, we found a significant association between greater numbers of alterations and poorer overall survival for multiple component genes. While the intricacies of CRL2pVHL complex gene regulation require additional research, it is evident that multiple causes for the deregulation of these genes must be considered in HCC, including non-traditional mechanisms.
Developing an Assessment of Contraceptive Preferences in Botswana: Piloting a Novel Approach Using Best-Worst Scaling of Attributes
Authors: Gertz Alida M. , Soffi Ahmad Syahir Mohd , Mompe Atlang , Sickboy Ontiretse , Gaines Averi N. , Ryan Rebecca , Mussa Aamirah , Bawn Caitlin , Gallop Robert , Morroni Chelsea , Crits-Christoph Paul
Published: May 2022

Introduction

To develop an attribute-based method for assessing patient contraceptive preferences in Botswana and pilot its use to explore the relationship between patient contraceptive preferences and the contraceptive methods provided or recommended to patients by clinicians.

Methods

A list of contraceptive attributes was developed with input from patients, clinicians, and other stakeholders. We assessed patient preferences for attributes of contraceptives using a discrete choice “best-worst scaling” approach and a multi-attribute decision-making method that linked patient attribute preferences to actual contraceptive method characteristics. Attribute-based patient method preferences and clinician recommendations were compared in 100 women seeking contraceptive services, and 19 clinicians who provided their care. For 41 of the patients, the short-term reliability of their preference scores was also examined.

Results

For 57 patients who wanted more children in the future, the degree of concordance between patients and clinicians was 7% when comparing the top attribute-based contraceptive preference for each woman with the clinician-provided/recommended method. When the top two model-based preferred contraceptive methods were considered, concordance was 28%. For 43 women who did not want more children, concordance was 0% when using the patient's model-based “most-preferred” method, and 14% when considering the top two methods. Assessment of the short-term reliability of preference scores yielded an intraclass correlation coefficient of 0.93.

Conclusions

A best-worst scaling assessment of attributes of contraceptives was designed and piloted in Botswana as a Contraceptive Preference Assessment Tool. The preference assessment was found to have high short-term reliability, which supports its potential use as a measurement tool. There was very low concordance between women's attribute-based contraceptive preferences and their clinician's provision/recommendations of contraceptive methods. Using such a preference assessment tool could encourage greater patient involvement and more tailored discussion in contraceptive consultations.
FcRn Antagonism Leads to a Decrease of Desmoglein-Specific B Cells: Secondary Analysis of a Phase 2 Study of Efgartigimod in Pemphigus Vulgaris and Pemphigus Foliaceus
Authors: Maho-Vaillant Maud , Sips Magdalena , Golinski Marie-Laure , Vidarsson Gestur , Goebeler Matthias , Stoevesandt Johanna , Bata-Csörgő Zsuzsanna , Balbino Bianca , Verheesen Peter , Joly Pascal , Hertl Michael , Calbo Sébastien
Published: May 2022

Background

Immunoglobulin G (IgG) levels are maintained by the IgG-recycling neonatal Fc-receptor (FcRn). Pemphigus vulgaris and pemphigus foliaceus are debilitating autoimmune disorders triggered by IgG autoantibodies against mucosal and epidermal desmogleins. Recently, a phase 2 clinical trial (NCT03334058;https://clinicaltrials.gov/NCT03334058) was completed in participants with pemphigus using efgartigimod, an FcRn inhibitor, in combination with prednisone. Efgartigimod demonstrated an early effect on diease activity and was well tolerated. In addition to the safety and efficacy assessment, clinical trials present an opportunity to gain more insights into the mechanism of disease, the mode of action of treatment, and potential for corticosteroid-sparing activity.

Objective

The aim of our study was to assess the impact of FcRn antagonism by efgartigimod on immunological parameters known to be directly involved in pemphigus pathology, such as cellular and serological responses.

Methods

We investigated total and antigen-specific IgG subclass level kinetics during and after treatment, assessed antigen-specific B-cell responses, followed T- and B-cell immunophenotypes, and analyzed how different immunophenotypes link to clinical response.

Results

Treatment resulted in reduction of total IgG as well as autoreactive IgG antibody levels. Surprisingly, unlike total IgG and vaccine- or natural-infection-elicited IgG, which returned to baseline levels after stopping efgartigimod treatment, autoreactive antibody levels remained low in several study participants. Efgartigimod showed no effect on total leukocytes, neutrophils, monocytes, or lymphocytes in patients treated with extended efgartigimod therapy. Intriguingly, antigen-specific analyses revealed a loss of desmoglein-specific B cells in several participants responding to efgartigimod, in line with prolonged reduction of pathogenic IgG levels.

Conclusions

Efgartigimod treatment of participants with pemphigus improved their conditions and exerted an immunomodulatory effect beyond the blockade of IgG recycling. Further studies in larger populations with an appropriate placebo control are needed to confirm these potentially important observations to establish long-term clinical responses in autoimmune diseases.
The Dual Functions of a Bracovirus C-Type Lectin in Caterpillar Immune Response Manipulation
Authors: Wu Xiaotong , Wu Zhiwei , Ye Xiqian , Pang Lan , Sheng Yifeng , Wang Zehua , Zhou Yuenan , Zhu Jiachen , Hu Rongmin , Zhou Sicong , Chen Jiani , Wang Zhizhi , Shi Min , Huang Jianhua , Chen Xuexin
Published: May 2022

Parasitoids are widespread in natural ecosystems and normally equipped with diverse viral factors to defeat host immune responses. On the other hand, parasitoids can enhance the antibacterial abilities and improve the hypoimmunity traits of parasitized hosts that may encounter pathogenic infections. These adaptive strategies guarantee the survival of parasitoid offspring, yet their underlying mechanisms are poorly understood. Here, we focused on Cotesia vestalis, an endoparasitoid of the diamondback moth Plutella xylostella, and found that C. vestalis parasitization decreases the number of host hemocytes, leading to disruption of the encapsulation reaction. We further found that one bracovirus C-type lectin gene, CvBV_28-1, is highly expressed in the hemocytes of parasitized hosts and participates in suppressing the proliferation rate of host hemocytes, which in turn reduces their population and represses the process of encapsulation. Moreover, CvBV_28-1 presents a classical bacterial clearance ability via the agglutination response in a Ca2+-dependent manner in response to gram-positive bacteria. Our study provides insights into the innovative strategy of a parasitoid-derived viral gene that has dual functions to manipulate host immunity for a successful parasitism.
Landscape of Metabolic Fingerprinting for Diagnosis and Risk Stratification of Sepsis
Authors: Lu Geng , Zhou Jiawei , Yang Ting , Li Jin , Jiang Xinrui , Zhang Wenjun , Gu Shuangshuang , Wang Jun
Published: May 2022

Background

Sepsis and septic shock, a subset of sepsis with higher risk stratification, are hallmarked by high mortality rates and necessitated early and accurate biomarkers.

Methods

Untargeted metabolomic analysis was performed to compare the metabolic features between the sepsis and control systemic inflammatory response syndrome (SIRS) groups in discovery cohort, and potential metabolic biomarkers were selected and quantified using multiple reaction monitoring based target metabolite detection method.

Results

Differentially expressed metabolites including 46 metabolites in positive electrospray ionization (ESI) ion mode, 22 metabolites in negative ESI ion mode, and 4 metabolites with dual mode between sepsis and SIRS were identified and revealed. Metabolites 5-Oxoproline, L-Kynurenine and Leukotriene D4 were selected based on least absolute shrinkage and selection operator regularization logistic regression and differential expressed between sepsis and septic shock group in the training and test cohorts. Respective risk scores for sepsis and septic shock based on a 3-metabolite fingerprint classifier were established to distinguish sepsis from SIRS, septic shock from sepsis. Significant relationship between developed sepsis risk scores, septic shock risk scores and Sequential (sepsis-related) Organ Failure Assessment (SOFA), procalcitonin (PCT) and lactic acid were observed.

Conclusions

Collectively, our findings demonstrated that the characteristics of plasma metabolites not only manifest phenotypic variation in sepsis onset and risk stratification of sepsis but also enable individualized treatment and improve current therapeutic strategies.
The Integrated Analysis Identifies Three Critical Genes as Novel Diagnostic Biomarkers Involved in Immune Infiltration in Atherosclerosis
Authors: Ye Zhen , Wang Xiao-kang , Lv Yun-hui , Wang Xin , Cui Yong-chun
Published: May 2022

Atherosclerosis (AS), a chronic inflammatory disease of the blood vessels, is the primary cause of cardiovascular disease, the leading cause of death worldwide. This study aimed to identify possible diagnostic markers for AS and determine their correlation with the infiltration of immune cells in AS. In total, 10 serum samples from AS patients and 10 samples from healthy subjects were collected. The original gene expression profiles of GSE43292 and GSE57691 were downloaded from the Gene Expression Omnibus database. Least absolute shrinkage and selection operator regression model and support vector machine recursive feature elimination analyses were carried out to identify candidate markers. The diagnostic values of the identified biomarkers were determined using receiver operating characteristic assays. The compositional patterns of the 22 types of immune cell fraction in AS were estimated using CIBERSORT. RT-PCR was performed to further determine the expression of the critical genes. This study identified 17 differentially expressed genes (DEGs) in AS samples. The identified DEGs were mainly involved in non-small cell lung carcinoma, pulmonary fibrosis, polycystic ovary syndrome, glucose intolerance, and T-cell leukemia. FHL5, IBSP, and SCRG1 have been identified as the diagnostic genes in AS. The expression of SCRG1 and FHL5 was distinctly downregulated in AS samples, and the expression of IBSP was distinctly upregulated in AS samples, which was further confirmed using our cohort by RT-PCR. Moreover, immune assays revealed that FHL5, IBSP, and SCRG1 were associated with several immune cells, such as CD8 T cells, naïve B cells, macrophage M0, activated memory CD4 T cells, and activated NK cells. Overall, future investigations into the occurrence and molecular mechanisms of AS may benefit from using the genes FHL5, IBSP, and SCRG1 as diagnostic markers for the condition.