bluuebunny/crossref_metadata_2025 huggingface dataset:
Advances in the microbiological diagnosis of herpetic retinitis
Authors: Gueudry Julie , Bodaghi Bahram
Published: September 2022

Viral retinitis associated with herpesvirus is one of the most severe forms of uveitis and is a potentially sight-threatening ophthalmologic disease. The prognosis is poor and a rapid and aggressive management is necessary to improve the visual and sometimes vital prognosis of these patients. The treatments used are not without side effects, while many differential diagnoses exist, such as toxoplasmic retinochoroiditis, syphilitic retinitis, endogenous endophthalmitis and intraocular lymphoma. Causatives viruses are herpes simplex virus, varicella-zoster virus, and cytomegalovirus, which require rapid detection in ocular fluid, mainly aqueous humor. However, only a small amount of intraocular fluid is available for analysis. Advances in microbiological diagnostic techniques therefore were key factors in improving the management of these diseases. Historically, the diagnosis was based on immunological tests but more recently advances in molecular biology, in particular polymerase chain reaction, have played a crucial role to obtain a reliable and rapid diagnosis of viral retinitis associated with herpesvirus, as discussed in this review.
Involvement of TLR2–TLR4, NLRP3, and IL-17 in pain induced by a novel Sprague-Dawley rat model of experimental autoimmune encephalomyelitis
Authors: Kwilasz Andrew J. , Clements Madison A. , Larson Tracey A. , Harris Kevin M. , Litwiler Scott T. , Woodall Brodie J. , Todd Laurel S. , Schrama Anouk E. W. , Mitten Eric H. , Maier Steven F. , Van Dam Anne-Marie , Rice Kenner C. , Watkins Linda R.
Published: September 2022

Up to 92% of patients suffering from multiple sclerosis (MS) experience pain, most without adequate treatment, and many report pain long before motor symptoms associated with MS diagnosis. In the most commonly studied rodent model of MS, experimental autoimmune encephalomyelitis (EAE), motor impairments/disabilities caused by EAE can interfere with pain testing. In this study, we characterize a novel low-dose myelin-oligodendrocyte-glycoprotein (MOG)-induced Sprague-Dawley (SD) model of EAE-related pain in male rats, optimized to minimize motor impairments/disabilities. Adult male SD rats were treated with increasing doses of intradermal myelin-oligodendrocyte-glycoprotein (MOG1−125) (0, 4, 8, and 16 μg) in incomplete Freund's adjuvant (IFA) vehicle to induce mild EAE. Von Frey testing and motor assessments were conducted prior to EAE induction and then weekly thereafter to assess EAE-induced pain and motor impairment. Results from these studies demonstrated that doses of 8 and 16 μg MOG1−125 were sufficient to produce stable mechanical allodynia for up to 1 month in the absence of hindpaw motor impairments/disabilities. In the follow-up studies, these doses of MOG1−125, were administered to create allodynia in the absence of confounded motor impairments. Then, 2 weeks later, rats began daily subcutaneous injections of the Toll-like receptor 2 and 4 (TLR2–TLR4) antagonist (+)-naltrexone [(+)-NTX] or saline for an additional 13 days. We found that (+)-NTX also reverses EAE-induced mechanical allodynia in the MOG-induced SD rat model of EAE, supporting parallels between models, but now allowing a protracted timecourse to be examined completely free of motor confounds. Exploring further mechanisms, we demonstrated that both spinal NOD-like receptor protein 3 (NLRP3) and interleukin-17 (IL-17) are necessary for EAE-induced pain, as intrathecal injections of NLRP3 antagonist MCC950 and IL-17 neutralizing antibody both acutely reversed EAE-induced pain. Finally, we show that spinal glial immunoreactivity induced by EAE is reversed by (+)-NTX, and that spinal demyelination correlates with the severity of motor impairments/disabilities. These findings characterize an optimized MOG-induced SD rat model of EAE for the study of pain with minimal motor impairments/disabilities. Finally, these studies support the role of TLR2–TLR4 antagonists as a potential treatment for MS-related pain and other pain and inflammatory-related disorders.
OVA-PEG-R848 nanocapsules stimulate neonatal conventional and plasmacytoid dendritic cells
Authors: Wirsching Sebastian , Machtakova Marina , Borgans Frauke , Pretsch Leah , Fichter Michael , Cacicedo Maximiliano L. , Thérien-Aubin Héloïse , Landfester Katharina , Gehring Stephan
Published: September 2022

Childhood mortality represents a major issue with 5. 3 million worldwide deaths of children under 5 years of age in 2019. Approximately half of those deaths can be attributed to easily preventable, infectious diseases. Currently approved neonatal vaccines are typically effective only after multiple doses leaving infants especially vulnerable during the first 6 months of life. Survival rates could be improved significantly by developing new and more potent vaccines that are capable of overcoming inherently tolerogenic neonatal immune systems. TLR agonists have garnered a great deal of attention in recent years due to their extensive capacities to activate innate immunity. Herein, the superior capacity of the TLR7/8 agonist, resiquimod (R848), to activate adult and neonatal primary peripheral blood dendritic cells is demonstrated. Moreover, R848 can be conjugated to polyethylene glycol and encapsulated in ovalbumin nanocapsules to efficiently co-deliver antigen and adjuvantin vitro. This study is among the first to demonstrate the capacity of encapsulated R848 to activate neonatal dendritic cells. These findings support the potential incorporation of R848 as adjuvant in neonatal vaccines, making them more effective in eliciting a robust immune response.
How sphingolipids affect T cells in the resolution of inflammation
Authors: Hartel Jennifer Christina , Merz Nadine , Grösch Sabine
Published: September 2022

The concept of proper resolution of inflammation rather than counteracting it, gained a lot of attention in the past few years. Re-assembly of tissue and cell homeostasis as well as establishment of adaptive immunity after inflammatory processes are the key events of resolution. Neutrophiles and macrophages are well described as promotors of resolution, but the role of T cells is poorly reviewed. It is also broadly known that sphingolipids and their imbalance influence membrane fluidity and cell signalling pathways resulting in inflammation associated diseases like inflammatory bowel disease (IBD), atherosclerosis or diabetes. In this review we highlight the role of sphingolipids in T cells in the context of resolution of inflammation to create an insight into new possible therapeutical approaches.
Efficacy of jianpi huatan granule in reducing colorectal cancer metastasis and recurrence after radical resection and adjuvant chemotherapy: Study protocol for a randomised, double-blind, placebo-controlled, multicentre trial
Authors: Li Liusheng , Qu Qian , Cui Ning , Cai Linlin , Zou Jianhua , Wu Jiao , Hao Tengteng , Wu Yu
Published: September 2022

Background: The high incidence and mortality rates of colorectal cancer (CRC) are a severe challenge in China. In patients with stage III and high-risk stage II CRC after radical resection and postoperative adjuvant chemoradiotherapy, 40–60% experience recurrence and metastasis. Several years of clinical practice have shown that traditional Chinese medicine, including Jianpi Huatan granule (JHG), effectively prevents stage III and high-risk stage II CRC recurrence and metastasis after radical resection and postoperative standard adjuvant chemotherapy. However, high-level systematic plans and evidence-based medicine are lacking in this regard. Therefore, this randomised control trial aimes to determine the efficacy of JHG in reducing stage III and high-risk stage II CRC metastasis and recurrence after radical resection and postoperative standard adjuvant chemotherapy.

Methods: This is a multicentre, randomised, double-blind, placebo-controlled clinical trial. Three hundred and fifty patients with stage III or high-risk stage II CRC who completed adjuvant chemotherapy after radical resection will be recruited from eight medical centres in China and randomly assigned to test (n = 175) and control (n = 175) groups at a ratio of 1:1. The test group will receive oral JHG for 3 months, whereas the control group will receive oral placebo for 3 months. The primary outcomes will be the disease-free survival and 1-, 2-, and 3-years metastasis and recurrence rates, whereas the secondary outcomes will be quality of life and circulating tumour cells. The patients will be followed-up monthly during treatment and every 3–6 months thereafter until recurrence, metastasis, death, or the end of the study.

Trial registration: This trial was registered at ClinicalTrials.gov (NCT03716063).
The regulatory mechanism and potential application of IL-23 in autoimmune diseases
Authors: Xiong De-Kai , Shi Xiang , Han Miao-Miao , Zhang Xing-Min , Wu Na-Na , Sheng Xiu-Yue , Wang Ji-Nian
Published: September 2022

IL-23 is a heterodimeric pro-inflammatory cytokine secreted by dendritic cells and macrophages that belongs to the IL-12 family. It has pro-inflammatory effects and is a key cytokine and upstream regulatory cytokine involved in protective immune responses, stimulating the differentiation and proliferation of downstream effectors such as Th17 cells. It is expressed in various autoimmune diseases such as psoriasis, systemic lupus erythematosus (SLE), rheumatoid arthritis (RA). The IL-23/TH17 axis formed by IL-23 and TH17 has been confirmed to participate in autoimmune diseases pathogenesis. IL-23R is the receptor for IL-23 and plays an activating role. Targeting IL-23 is currently the main strategy for the treatment of various autoimmune diseases. In this review we summarized the mechanism of action and clinical application potential of IL-23 in autoimmune diseases by summarizing the latest research results and reviewing the literature, which would help to further understand IL-23 and provide a theoretical basis for future clinical targeting and drug development.
Traditional Chinese decoction Si Zhi Wan attenuates ovariectomy (OVX)-induced bone loss by inhibiting osteoclastogenesis and promoting apoptosis of mature osteoclasts
Authors: He Qingman , Fu Kanghua , Yao Huan , Wei Shujun , Xiang Li , Liu Sixian , Chen Tao , Gao Yongxiang
Published: September 2022

Si Zhi Wan (SZW) is a traditional Chinese decoction used for osteoporosis treatment. Currently, the effect of SZW on ovariectomy (OVX)-induced bone loss and the underlying mechanisms remain unknown. Herein, we investigated the therapeutic effect of SZW on osteoporosis and explored the underlying mechanisms in vitro and in vivo. An OVX-induced bone loss model was established in vivo. After administration of SZW for 8 weeks, rats were sacrificed, and the uterus was weighted to calculate its index. The femur change was pathologically evaluated using hematoxylin and eosin (H&E) staining. The mineral density of the femur was observed by micro-CT. RAW264.7 cells were activated by receptor activator of nuclear factor-κB ligand (RANKL) in vitro. The effect of SZW on osteoclastogenesis was evaluated using tartrate-resistant acid phosphatase (TRAP) staining, Western blotting, and RT-PCR. The pro-apoptosis effect of SZW on mature osteoclasts was examined after induction of osteoclast maturation. Finally, the effect of SZW on the NF-κB pathway was evaluated. Our results demonstrated that SZW ameliorated OVX-induced bone loss in rats. In addition, SZW inhibited osteoclastogenesis and attenuated osteoclast-mediated bone resorption in vitro and in vivo. SZW also promoted apoptosis of mature osteoclasts. Mechanically, SZW exerts its effects by suppressing the NF-κB pathway. Overall, these findings demonstrated that SZW may be a potentially effective alternative treatment for osteoporosis.
Chicken white egg chemerin as a tool for genetic selection for egg weight and hen fertility
Authors: Bernardi Ophélie , Reverchon Maxime , Estienne Anthony , Baumard Yannick , Ramé Christelle , Brossaud Adeline , Combarnous Yves , Froment Pascal , Dupont Joëlle
Published: September 2022

Embryo mortality rate, which can reach up to 40% in avian species, is a major issue for breeding. It is therefore important to identify new embryo development biomarkers for genetic selection to improve reproductive performances. We have recently shown that chemerin is expressed in the oviductal hen magnum, accumulates in egg white, is correlated with embryo survival and could thus be used as a molecular marker of embryo development. Eggs from seven hen breeds (n= 70) were collected during five successive days at the end of the laying period. After weighing eggs, yolk and albumen, an egg white sample from each egg was collected and a blood sample was taken from each hen. Chemerin concentrations in albumen and blood samples were measured by a specific home made ELISA assay. Hen’s plasma and egg’s albumen chemerin levels were found to be correlated with reproductive parameters such as fecundity, fertility, embryo mortality, hatchability and laying rates. The inter-hen chemerin level variability in albumen was higher than intra-hen except for one breed (R+). We observed significantly different levels of chemerin in egg white between breeds. However, chemerin concentrations in egg white were not significantly associated to variations of hen plasma chemerin levels. Interestingly, we observed negative correlations between albumen chemerin concentrations and egg weight (r= −0.43,p= 0.001), between albumen weight (r= −0.40,p= 0.002), and between yolk weight (r= −0.28,p= 0.03). We also showed negative correlations between egg white chemerin concentrations and fecundity (r= −0.32,p= 0.011) and fertility (r= −0.27,p= 0.04) whereas no significant correlation was observed with the laying rate. Taken together, these results suggest that egg white chemerin concentration might be a good biomarker for genetic selection for egg weight and fertility in hens, provided these data are confirmed on a larger scale.
Haptoglobin treatment contributes to regulating nitric oxide signal and reduces oxidative stress in the penis: A preventive treatment for priapism in sickle cell disease
Authors: Pereira Pamela da Silva , Pereira Dalila Andrade , Calmasini Fabiano Beraldi , Reis Leonardo O. , Brinkman Nathan , Burnett Arthur L. , Costa Fernando Ferreira , Silva Fábio Henrique
Published: September 2022

Background: In sickle cell disease (SCD), reduced bioavailability of endothelial NO and cGMP results in reduced expression of phosphodiesterase type 5 (PDE5), thus impairing the penile erection control mechanism and resulting in prolonged penile erection (priapism). In SCD, reduced NO bioavailability is associated with excess plasma hemoglobin due to intravascular hemolysis and increased oxidative stress. Haptoglobin is the plasma protein responsible for reducing plasma hemoglobin levels, but in SCD, haptoglobin levels are reduced, which favors the accumulation of hemoglobin in plasma. Therefore, we aimed to evaluate the effects of haptoglobin treatment on functional and molecular alterations of erectile function, focusing on the contractile and relaxant mechanisms of corpus cavernosum (CC), as well as oxidative stress.

Methods: SCD mice were treated with haptoglobin (400 mg/kg, subcutaneous) or vehicle of Monday, Wednesday and Friday for a period of 1 month. Corpus cavernosum strips were dissected free and placed in organ baths. Cumulative concentration-response curves to the acetylcholine, sodium nitroprusside, phenylephrine and KCL, as well as to electrical field stimulation (EFS), were obtained in CC. Protein expressions of eNOS, phosphorylation of eNOS at Ser-1177, nNOS, PDE5, ROCK1, ROCK2, gp91phox, 3-nitrotyrosine, and 4-HNE were measured by western blot in CC.

Results: Increased CC relaxant responses to acetylcholine, sodium nitroprusside and electrical-field stimulation were reduced by haptoglobin in SCD mice. Reduced CC contractile responses to phenylephrine and KCl were increased by haptoglobin in SCD mice. Haptoglobin prevented downregulated eNOS, p-eNOS (Ser-1177), PDE5, and ROCK2 protein expressions and reduced protein expressions of reactive oxygen species markers, NADPH oxidase subunit gp91phox, 3-nitrotyrosine and 4-HNE in penises from SCD mice. Haptoglobin treatment did not affect ROCK1 and nNOS protein expressions in penises from SCD mice. Basal cGMP production was lower in the SCD group, which was normalized by haptoglobin treatment.

Conclusion: Treatment with haptoglobin improved erectile function due to up-regulation of eNOS-PDE5 expression and down-regulation of the gp91phox subunit of NADPH oxidase and oxidative/nitrosative stress in the penises of SCD mice. Treatment with haptoglobin also increased contractile activity due to up-regulation of ROCK2. Therefore, haptoglobin treatment may be an additional strategy to prevent priapism in SCD.
Exploring the role of mast cells in the progression of liver disease
Authors: Huang Shizhuan , Wu Haotian , Luo Feng , Zhang Bin , Li Tianwei , Yang Zongrui , Ren Bixuan , Yin Wenze , Wu Dehai , Tai Sheng
Published: September 2022

In addition to being associated with allergic diseases, parasites, bacteria, and venoms, a growing body of research indicates that mast cells and their mediators can regulate liver disease progression. When mast cells are activated, they degranulate and release many mediators, such as histamine, tryptase, chymase, transforming growth factor-β1 (TGF-β1), tumor necrosis factor–α(TNF-α), interleukins cytokines, and other substances that mediate the progression of liver disease. This article reviews the role of mast cells and their secretory mediators in developing hepatitis, cirrhosis and hepatocellular carcinoma (HCC) and their essential role in immunotherapy. Targeting MC infiltration may be a novel therapeutic option for improving liver disease progression.
The differential impact of COVID-19 on mental health: Implications of ethnicity, sexual orientation, and disability status in the United States
Authors: Brooks Jordan M. , Patton Cyrano , Maroukel Sharon , Perez Amy M. , Levanda Liya
Published: September 2022

The COVID-19 pandemic’s effects on mental health interact with preexisting health risks and disparities to impact varying populations differently. This study explored the relationship between demographic variables (e.g., ethnicity, sexual orientation, and disability status), distress and mental health (e.g., depression, anxiety, somatic complaints, and pandemic distress), and vulnerability factors for COVID-19 (e.g., personal health vulnerabilities, community members’ health vulnerabilities, and environmental exposure risks at work or home). An online cross-sectional study was conducted from 18 June to 17 July 2020, reflecting the impact of early phase COVID-19 pandemic and related shelter-in-place measures in the United States. Participants were adults residing in the United States (N = 594), with substantial subsamples (N ≥ 70) of American Indian, Asian American, African-American, and Hispanic and/or Latinx participants, as well as people with disabilities and sexual minorities. Outcomes measured were depression, hopelessness, somatic complaints, anxiety-related disorders, locus of control (LOC), and a novel measure of pandemic-related distress. Data were analyzed using analyses of covariance (ANCOVA), chi-square test, and correlation coefficients. Generally, younger individuals, and those with less financial power—across all identities—suffered more distress. When controlling for age, lower financial power was associated with higher scores on the Center for Epidemiologic Studies Depression Scale-Revised (CESD-R; r = –0.21, p = < 0.001), Beck Hopelessness Scale (BHS; r = –0.17, p < 0.001), Patient Health Questionnaire-15 (PHQ-15; r = –0.09, p = 0.01), Screen for Child Anxiety Related Emotional Disorders for Adults Panic Disorder (SCARED-A PD; r = –0.14, p < 0.001), SCARED-A generalized anxiety disorder (GAD; r = –0.13, p = 0.002), SCARED-A obsessive–compulsive disorder (OCD; r = –0.08, p = 0.04), and the COVID-19 Pandemic Distress restriction/disconnection scale (C19PDS; r = –0.10, p = 0.009). In addition, disparities were found, in general, for marginalized identities by gender, sexual orientation, and disability status. Importantly, each ethnicity subsample showed a unique pattern of relationships between COVID-19 risk variables and mental health symptoms. The results support the hypothesis that any pandemic may amplify preexisting social and financial disparities. Overall, interventions at the clinical, governmental, or health equity level should take into consideration the needs of vulnerable groups.
Students' Performances in Computer Programming of Higher Education for Sustainable Development: The Effects of a Peer-Evaluation System
Authors: Hsiao Tsung-Chih , Chuang Ya-Hsueh , Chen Tzer-Long , Chang Chien-Yun , Chen Chih-Cheng
Published: September 2022

Modern education attaches great importance to interdisciplinary skills, among which computational thinking is a core element, and heralds a new era. IT application has shaped education in the 21st century. Computational thinking has provided further impetus for building an all-encompassing social network and fostering a DIY culture enabled by digital technologies. One empirical study used four apps to test children's development in computational thinking and fluency. The article will help students overcome their fears of coding. Peer reviews provide students with an opportunity to learn from each other and become more motivated. These reviews also serve as feedback for teachers to evaluate students' performance. Experimental design is used in this study, and a peer review system is implemented. Freshmen attending a programming class in a university are used as samples. At the class, students write computer programs with f-Chart, which provides a graphical user interface for students to learn programming logic and design. Zuvio, a cloud-based interactive response system, is used to conduct the peer reviews. The data of this study are analyzed through R. The results show not only an improvement in students' learning performance but also a gap between students' peer review scores and teachers' evaluation scores. Learning feedback and evaluation is crucial to transform education between students and teachers into a sustainable cycle system.
Are the concepts of emotion special? A comparison between basic-emotion, secondary-emotion, abstract, and concrete words
Authors: González-Arias Mauricio , Aracena Daniela
Published: September 2022

The study of emotional concepts stands at a very interesting intersection between the theoretical debate about the nature of emotions and the debate about the nature of processing concrete concepts and abstract concepts. On the one hand, it is debated whether it is possible to differentiate basic emotions from secondary emotions and, on the other hand, whether emotional concepts differ from abstract concepts. In this regard, the prototypical perceptual aspects are considered an important factor both for the differentiation between concrete and abstract concepts and for the differentiation between basic and secondary emotions (facial expressions). Thus, the objective has been to determine if (a) the presence or absence of a prototypical perceptual referent, and (b) the type of concept (referring to emotion and not referring to emotion), produce differences between concepts of basic emotions, secondary emotions and concepts not related to emotions, concrete and abstract, in the tasks of qualification of concreteness, imageability and availability of context and the task of the list of properties, that have been used in previous studies. A total of 86 university students from the suburbs of La Serena - Coquimbo (Chile), all native Spanish speakers, participated in the study. The results show that in the perception of concreteness and in the total of enumerated properties, emotional concepts presented similar results to abstract concepts not related to emotion and there was no difference between basic and secondary emotion concepts. In imageability and context availability, emotional concepts were perceived as different from and more concrete than abstract concepts. In addition, the cause-effect type attributes allowed to clearly differentiate emotional concepts from those not related to emotion and to differentiate between basic and secondary emotion concepts. These types of attributes appear almost exclusively in emotional concepts and are more frequent in basic emotions. These results are partially consistent with the predictions of Neurocultural and Conceptual Act theories about emotions.
Appearing competent or moral? The role of organizational goals in the evaluation of candidates
Authors: Fousiani Kyriaki , Van Prooijen Jan-Willem , Armenta Bibiana
Published: September 2022

The Big Two theoretical framework suggests that two traits, namely morality and competence, govern social judgments of individuals and that morality shows a primacy effect over competence because it has more diagnostic value. In this study we tested the primacy effect of morality in the workplace by examining how instrumental or relational goals of organizations might influence the importance of morality or competence of candidates during the hiring process. We hypothesized that the primacy effect of morality might hold when organizational goals are relational, but it might get reversed when organizational goals are instrumental. Supporting our hypothesis, in a field study and two experiments (both preregistered) we found that people perceive moral candidates as more appropriate for recruitment when an organization prioritizes relational goals (Studies 1, 2, and 3). In contrast, people perceive competent candidates as more appropriate for recruitment when an organization prioritizes instrumental goals (Studies 1 and 2). Perceived appropriateness of a candidate, in turn, predicts a stronger intention to recruit a candidate (Studies 2 and 3). These results provide evidence for a reversal of the primacy effect of morality in a work setting, and illuminate the important role of organizational goals in social judgments.
Understanding the drivers of the public value of e-government: Validation of a public value e-government adoption model
Authors: Mensah Isaac Kofi , Zeng Guohua , Mwakapesa Deborah Simon
Published: September 2022

This study examined the factors driving the public value of e-government from the viewpoint of the Chinese people. The usage of ICT through e-government systems must generate the adequate corresponding public value that can motivate the acceptance of e-government services. The sample 517 data generated from Chinese citizens were analyzed using AMOS 23 software by undertaking the structural equation model system of analysis. The results show that constructs such as information quality, service parameters, user orientation, efficiency, openness, and responsiveness were significantly related to the public value of e-government. Additionally, the research validated that the public value of e-government has a direct influence on the behavioral intention to adopt e-government services. The managerial and practical implications of these research findings on the public value of e-government and the acceptance of e-government services are dissected meticulously.
Understanding cognitive differences in the effect of digitalization on ambidextrous innovation: Moderating role of industrial knowledge base
Authors: Xu Qiang , Liu Hanlin , Chen Yi , Tian Kexin
Published: September 2022

A number of existing researches agree that digitalization would facility firms to launch ambidextrous innovations. Digitalization is not only about technological change, but more importantly, the reshaping of the firms’ knowledge structure and routines to percept and integrate knowledge. Thus, some researchers suggest that whether firms could benefit from digitalization varies across firms and industries, since innovation in different firms and industries relies on differentiated level of cognitive and reasoning of knowledge. However, existing studies mainly focus on exploring the firm-level differences, and leave the industry-level difference underdeveloped. In response, this study integrates knowledge-based view to examine how a firm’s digitalization affects ambidextrous innovation, and further explore the conjoint effect of industrial knowledge bases—the knowledge base of the industry the firm is located in—on the relationship between firm digitalization level and ambidextrous innovation. This study uses Python to conduct text mining of firms’ annual reports, and obtains data of 394 listed companies from the year 2014 to 2020. The empirical results show that digitalization level has positive effect on both exploratory innovation and exploitative innovation, and the effect on exploitative innovation is stronger than on exploratory innovation. Moreover, the moderating effect of industrial knowledge base is significant on “digitalization–exploratory innovation” but not on “digitalization–exploitative innovation” relationship. By doing so, this study refines the research on the relationship between digitalization and firm innovation, and confirms that the usage of digitalization may lead to achieve an ambidextrous situation. This study also provides a theoretical basis for industrial differences of the effectiveness of digitalization, suggesting firms considering industrial characteristics to implement digitalization-assisted innovation practices.
How do people with physical disabilities want to construct virtual identities with avatars?
Authors: Park Jaeyoung , Kim Seongcheol
Published: September 2022

In the virtual world, people can reconstruct their identity the way they want with avatars. Many expect the high degree of freedom in avatar customization will give new chances to socially marginalized people experiencing discrimination against their physical traits. Accordingly, research on a virtual embodiment of marginalized people has been steady with increased interest in equity and inclusion. However, even discourse alienates people with disabilities. In addition, there are few studies on the virtual representations of people with disabilities. Therefore, this paper explores the shared perception of avatar-based disability representations among people with disability to help understand how they want to construct their disability with avatars. The study also gives direction for a barrier-free virtual world. We conducted semi-structured in-depth interviews with people with physical impairments who used virtual world services and performed a core-periphery analysis of social representations. This study expands the range of academic adoption of the social representations theory and provides insights for stakeholders such as service providers to design an inclusive virtual world.
Effect of SIRT1 on white matter neural network in adolescent patients with depression
Authors: Ji Ling , Jiang Wen , Liu Daiyan , Hou Kaiwen
Published: September 2022

Background

This study aimed to explore the correlation between the rs12415800 polymorphism of the silent information regulator 1 (SIRT1) gene and the white matter neural circuit in adolescent patients with depression.

Methods

We enrolled 119 participants, comprising 59 adolescent patients with depression and 60 matched healthy controls for analysis. Patients were further subdivided based on genotype; GG, AG, and AA, with G representing the wild type gene, and A representing the A allele at rs12415800.

Results

We found that: (1) lower anisotropy fraction (FA) values in the left cingulate fasciculus and left anterior thalamus radiation in the AG/AA genotype were more likely to be affected by depression. (2) The FA values of the right inferior occipital-frontal fasciculus, right corticospinal tract, right inferior longitudinal fasciculus, and right superior longitudinal fasciculus regions in the depression (AG/AA) group were lower than in the depression (GG) group. (3) FA values of the right inferior occipital-frontal fasciculus left corticospinal tract, right inferior longitudinal fasciculus, left anterior thalamus radiation, right superior longitudinal fasciculus, left inferior longitudinal fasciculus, left uncinate fasciculus, and right anterior thalamus radiation in the depression (GG) group were lower than the control (GG) group.

Conclusions

The polymorphism locus of the SIRT1 gene rs12415800 may be related to changes in the microstructure of white matter fiber tracts, and patients carrying the A allele (AG/AA) have more changes in the white matter than those with the non-A allele (GG).
Management and treatment of long COVID symptoms in general practices: An online-based survey
Authors: Schrimpf Anne , Braesigk Annett , Lippmann Stefan , Bleckwenn Markus
Published: September 2022

Independent from initial severity, many patients develop persistent symptoms after infection with SARS-CoV-2, described as long COVID syndrome. Most of these patients are treated by general practitioners (GPs). As evidence-based treatment recommendations are still sparse, GPs must make their therapy decisions under uncertainty. We investigated (1) the most frequently observed long COVID symptoms in general practices and (2) GPs' applied treatment and rehabilitation plans for these symptoms. In total, 143 German GPs participated in an online-based survey between 05/2021 and 07/2021. We found that each GP practice was treating on average 12 patients with long COVID symptoms. Most frequently seen symptoms were fatigue and reduced performance. Current therapy options were rated as poor and loss of smell and taste, fatigue, or lack of concentration were perceived to be especially difficult to treat. The use of drug and non-drug therapies and specialist referrals focused primarily on physiological and less on psychosomatic/psychological rehabilitation and followed guidelines of similar conditions. Our results provide first insights into how GPs approach a newly emerging condition in the absence of guidelines, evidence-based recommendations, or approved therapies, and might inform about GP preparedness in future pandemics. Our results also emphasize a gap between the current knowledge of the long COVID manifestation and knowledge about effective rehabilitation.
Food security status of Suchana-participating households in north-eastern rural Bangladesh
Authors: Haque Md Ahshanul , Choudhury Nuzhat , Ahmed S. M. Tanvir , Farzana Fahmida Dil , Ali Mohammad , Naz Farina , Khan Ashfaque , Wahid Barbie Zaman , Siddiqua Towfida Jahan , Akter Rumana , Rahman Sheikh Shahed , Faruque A. S. G. , Ahmed Tahmeed
Published: September 2022

Despite achieving remarkable progress, food insecurity remains a major public health challenge in Bangladesh, and severe food insecurity status has not been reduced in susceptible areas and vulnerable regions in Bangladesh. Wetlands that are susceptible to flooding can be found in Bangladesh's north-eastern Sylhet division. Suchana, a large-scale nutrition programme, implemented nutrition-specific and sensitive interventions in poor and very poor households in Sylhet and Moulvibazar districts in the north-eastern region of Bangladesh. The aim of this article is to assess the association between the Suchana intervention and household food security status among poor and very poor households in north-eastern rural Bangladesh using the Suchana baseline and endline survey databases. The baseline survey was conducted between November 2016 and February 2017, while the endline survey was undertaken 3 years later, during the same months. The outcome variable in this analysis was household food security status, which was measured using the Food and Nutrition Technical Assistance's Guideline. Descriptive statistics were used to summarize the data; after controlling for the union as a cluster and relevant covariates, a multiple multinomial logistic regression model was used to estimate the independent effect of the Suchana intervention as an exposure. Overall, 14.0% of households were food secure at the baseline survey (intervention: 14.1%, control: 14.0%) and 22.0% were food secure (intervention: 26.6%, control: 20.2%) at the endline survey. For households in the intervention area in comparison to the control area, the odds of being moderately food insecure [aOR: 1.36 (1.05, 1.76), p < 0.05], mildly food insecure [aOR: 1.83 (1.33, 2.51), p < 0.001], or food secure [aOR: 2.21 (1.47, 3.33), p < 0.001] compared to being severely food insecure was significantly higher. Thus, we infer that the 3 years of Suchana intervention marginally increased household food security status among the socio-economically disadvantaged population in north-eastern rural Bangladesh. If concerns regarding gender equity, women's education, and income-generating activities are addressed, the population could experience even greater benefits in food security. In order to overcome these challenges, all stakeholders including programme implementers and policymakers should work together to implement the appropriate measures.
Stigma toward people with COVID-19 among Bangladeshi older adults
Authors: Mistry Sabuj Kanti , Ali A. R. M. Mehrab , Yadav Uday Narayan , Huda Md. Nazmul , Rahman Md. Mahmudur , Saha Manika , Rahman Md. Ashfikur , Lim David , Ghimire Saruna
Published: September 2022

The onset of the coronavirus disease (COVID-19) pandemic and its overwhelming physical and mental health burden can result in stigmatization toward the disease and those affected. This study aimed to measure the prevalence of COVID-19-related stigma and its associated factors among older people in Bangladesh. This cross-sectional study was conducted among 1,045 Bangladeshi older adults aged 60 years and above through telephone interviews in September 2021. The outcome was measured using an eight-point Stigma Scale, adapted to the Bengali language. Level of stigma was indicated by the cumulative score of the eight-items, ranging from 0 to 8, with a higher score indicating a higher level of stigma. On average, participants had stigmas on three of the eight items, and 62.6% had a high stigma score. The most prevalent stigmas were as follows: COVID-19 is a punishment from God (79.3%), patients with previous COVID-19 must be isolated (67.3%), and people infected with COVID-19 did not meet hygiene standards (63.9%). Participants who lived in rural areas (β: 0.67, 95% CI: 0.39 to 0.95) and who perceived needing additional care during the pandemic (β: 0.35, 95% CI: 0.09 to 0.60) had a higher average stigma score, whereas stigma scores were lower among unemployed/retired participants (β: −0.22, 95% CI: −0.45 to 0.00). The study findings suggest implementing interventions to raise awareness through appropriate health literacy interventions and mass media campaigns.
Cold-Loving Bacteria Can Survive in the Ice
Authors: Chang Kristy Yi Wen , Tanoto Frederick Reinhart , Lopes dos Santos Adriana
Published: September 2022

Did you know that there are many places on Earth that are frozen all year round? Can you imagine living your whole life in the bitter cold of snow and ice? Well, not only can some tiny microbes live comfortably in the cold, but many kinds of bacteria call such harsh places home! Just as a polar bear needs a thick coat to survive in the cold, these bacteria have their own ways of keeping themselves comfortable in the harsh environment. Scientists have found that these bacteria can teach us a lot about life in our solar system and that they are extremely useful in many industries.
Insights into the diversity of cow milk production systems on the fringes of coastal cities in West Africa: A case study from Benin
Authors: Yassegoungbe Fifame Panine , Oloukoi David , Aoudji Augustin K. N. , Schlecht Eva , Dossa Luc Hippolyte
Published: September 2022

In sub-Saharan Africa, rapid population growth, urbanization, increasing incomes, and changing dietary preferences are the main drivers of the rising demand for livestock products, especially fresh milk and derived products. To meet this demand, there is an increasing number of dairy cattle farms in the densely populated coastal zone of Benin, where the country's largest city and commercial capital Cotonou is located. To identify and characterize the peri-urban dairy production systems in this region, 190 cattle keepers were surveyed, using the snowball sampling method, in four municipalities neighboring Cotonou. Information on their socio-economic characteristics, cattle herd sizes, and herd management practices were collected through questionnaire-based face-to-face interviews. Factor analysis of mixed data followed by hierarchical clustering on principal components, implemented in R statistical software, were applied to classify the surveyed farms into homogeneous groups. Results revealed six types of peri-urban dairy cattle farms differing mainly in their cows' breeds, herd sizes, and daily amount of milk produced. Most herds (88%) were owned by urban dwellers, mainly civil servants and traders, who entrusted the management of their cattle to hired professional herders. Irrespective of farm type, cows were of local taurine (65%) or Sahelian zebu (35%) breeds and were exclusively fed on communal natural pasture. Mineral supplementation was provided to the animals on 42% of farms, with significant variation across farm types. About 45% of the farms integrated cattle production with other agricultural activities, including coconut plantations (22%), where cow manure was used as fertilizer. The herd structure was similar across farm types, with average proportions of cows and heifers ranging from 37.6 to 47.5% and from 13.1 to 19.7%, respectively. With significant differences across farm types, the produced milk was either transformed into traditional cheese (32% of farms) or sold raw (85%). Milk and cheese sales represented 84% of the total farm income for three out of the six farm types. In the current context of rapid urbanization, communal grazing lands alone cannot provide sufficient feed to support increased milk production. In addition to improved feeding strategies, herd structure should be balanced in terms of the ratio between milk-producing and non-producing animals.
BioGlue® is not associated with polypropylene suture breakage after aortic surgery
Authors: Pacini Davide , Murana Giacomo , Hollinworth David , Northrup William F. , Arnold Stacy G. , Di Bartolomeo Roberto
Published: September 2022

Objective

We have encountered broken or damaged polypropylene sutures (Prolene®) at the anastomotic sites during aortic reoperations. Because a surgical sealant, bovine serum albumin-glutaraldehyde (BioGlue®), was used in previous aortic surgery in some of these cases, we undertook this in vitro study to evaluate whether the use of BioGlue® was associated with breakage of polypropylene sutures at the aortic anastomosis.

Materials and methods

The broken polypropylene sutures, anastomotic sites and aortic tissue at the location of suture breakage were visually inspected and evaluated intraoperatively. Six human cadaveric aortic samples were incised circumferentially and anastomosed proximally to a valved conduit with running 4–0 polypropylene sutures (Prolene®). In the test group (n = 3), BioGlue® was applied directly to the Prolene® sutures at the anastomotic sites, while in the control group (n = 3) the anastomoses were not sealed with any surgical adhesive. The six samples were immersed in Dulbecco's phosphate buffered saline solution and mounted on a M-6 Six Position Heart Valve Durability Testing System and tested up to 120 million cycles for a 2-year period. During and upon completion of the testing, the integrity of Prolene® sutures, the anastomosis and aortic tissues was regularly assessed by visual inspection.

Results

Intraoperative findings included a stretched and thin aortic wall (some with thrombus), a small cleft between the aortic tissue and the Dacron vascular graft. An excessive amount of BioGlue® was often found around the anastomosis, with cracking material, but no signs of mechanical damage were observed in these cases. Upon visual inspection during and after in vitro testing, there was no apparent damage to the polypropylene sutures on the interior or exterior of the aortic anastomoses in any of the samples. No difference was observed in the physical integrity of the polypropylene sutures at anastomotic lines, the anastomoses and aortic tissues between the test and control samples.

Conclusions

The results of this study suggest that the use of BioGlue® was not associated with breakage of the polypropylene sutures at the anastomotic sites after aortic dissection repair.
Prognostic value of lactate in cats presented in respiratory distress to the emergency room
Authors: Gilday Cassandra , Guieu Liz
Published: September 2022

Studies demonstrating the prognostic utility of plasma lactate concentration and lactate clearance in cats are limited. The objective of this study was to determine the prognostic utility of plasma lactate concentration upon admission and plasma lactate clearance in cats presented to the emergency room with respiratory distress attributed to underlying cardiac or respiratory disease. Additionally, we sought to determine if plasma lactate concentration on admission was significantly associated with the underlying cause of respiratory distress (cardiac vs. respiratory), type of respiratory disease, and vital parameters. Seventy-one cats presented in respiratory distress to the ER at a university teaching hospital were enrolled in this retrospective study. Admission lactate concentration was not associated with survival, duration of hospitalization, vital parameters, or underlying etiology for respiratory distress. In contrast, lactate clearance was significantly associated with survival and length of hospitalization. While a statistically significant association between lactate clearance and length of hospitalization was identified, this finding lacked clinical significance where an increase in lactate concentration by 1% was associated with an increased length of hospitalization by 11.4 min (p = 0.035). Results of this study suggest that lactate clearance may have prognostic utility in this population of cats. Further studies including the larger population of cats with underlying cardiac or respiratory disease are warranted.