bluuebunny/crossref_metadata_2025 huggingface dataset:
Is Subjective Knowledge the Key to Fostering Sustainable Behavior? Mixed Evidence from an Education Intervention in Mexico
Authors: Redman Aaron , Redman Erin
Published: December 2016

Educational interventions are a promising way to shift individual behaviors towards Sustainability. Yet, as this research confirms, the standard fare of education, declarative knowledge, does not work. This study statistically analyzes the impact of an intervention designed and implemented in Mexico using the Educating for Sustainability (EfS) framework which focuses on imparting procedural and subjective knowledge about waste through innovative pedagogy. Using data from three different rounds of surveys we were able to confirm (1) the importance of subjective and procedural knowledge for Sustainable behavior in a new context; (2) the effectiveness of the EfS framework and (3) the importance of changing subjective knowledge for changing behavior. While the impact was significant in the short term, one year later most if not all of those gains had evaporated. Interventions targeted at subjective knowledge will work, but more research is needed on how to make behavior change for Sustainability durable.
A Miniature Data Repository on a Raspberry Pi
Authors: Samourkasidis Argyrios , Athanasiadis Ioannis
Published: December 2016

This work demonstrates a low-cost, miniature data repository proof-of-concept. Such a system needs to be resilient to power and network failures, and expose adequate processing power for persistent, long-term storage. Additional services are required for interoperable data sharing and visualization. We designed and implemented a software tool called Airchive to run on a Raspberry Pi, in order to assemble a data repository for archiving and openly sharing timeseries data. Airchive employs a relational database for storing data and implements two standards for sharing data (namely the Sensor Observation Service by the Open Geospatial Consortium and the Protocol for Metadata Harvesting by the Open Archives Initiative). The system is demonstrated in a realistic indoor air pollution data acquisition scenario in a four-month experiment evaluating its autonomy and robustness under power and network disruptions. A stress test was also conducted to evaluate its performance against concurrent client requests.
Resilience Evaluation of Demand Response as Spinning Reserve under Cyber-Physical Threats
Authors: AlMajali Anas , Viswanathan Arun , Neuman Clifford
Published: December 2016

In the future, automated demand response mechanisms will be used as spinning reserve. Demand response in the smart grid must be resilient to cyber-physical threats. In this paper, we evaluate the resilience of demand response when used as spinning reserve in the presence of cyber-physical threats. We quantify this evaluation by correlating the stability of the system in the presence of attacks measured by system frequency (Hz) and attack level measured by the amount of load (MW) that responds to the demand response event. The results demonstrate the importance of anticipating the dependability of demand response before it can be relied upon as spinning reserve.
A Compressive-Sensing Inspired Alternate Projection Algorithm for Sparse Array Synthesis
Authors: Pinchera Daniele , Migliore Marco , Lucido Mario , Schettino Fulvio , Panariello Gaetano
Published: December 2016

In this paper, we propose a simple approach for sparse array synthesis. We employ a modified generalized alternate projection algorithm using ℓ 1 -norm constrained minimization in order to achieve the excitation and the position of the elements of a sparse array. The proposed approach is very flexible, since it deals with power pattern masks and allows the inclusion of the effects of element pattern and mutual coupling. Its implementation is relatively simple, thanks to the possibility to use well-known convex programming techniques. The presented method is particularly suitable for the synthesis of patterns commonly employed in radar systems; the numerical results provided show good performances with respect to concurrent methods available in open literature.
Experimental Investigation on a Thermal Model for a Basin Solar Still with an External Reflector
Authors: Afrand Masoud , Kalbasi Rasool , Karimipour Arash , Wongwises Somchai
Published: December 2016

In this study, a thermal model for estimating the efficiency of a basin solar still with an external reflector was introduced using the energy balance equations of different parts of the solar still. Then, in order to verify the precision and accuracy of this model, a basin solar still with an external reflector was constructed and some experiments were performed. The hourly temperature values for different places of the still and amount of distilled water were calculated using the thermal model and compared with experimental measurements. Comparisons show that the thermal model of the still is in good agreement with the experimental results. Therefore, it can be concluded that the introduced thermal model can be used reliably to estimate the amount of distilled water and efficiency of the basin solar still with an external reflector. Results also revealed that the efficiency of the solar still is low in the early hours, while it was enhanced 44% in the afternoon. Furthermore, it was concluded that the accumulated distilled water is 4600 mL/day and 4300 mL/day for theoretical and experimental examinations, respectively.
A Simple Method for the Detection of Long-Chain Fatty Acids in an Anaerobic Digestate Using a Quartz Crystal Sensor
Authors: Kobayashi Takuro , Kuramochi Hidetoshi , Maeda Kouji , Xu Kaiqin
Published: December 2016

In anaerobic digestion (AD), long-chain fatty acids (LCFAs) produced by hydrolysis of lipids, exhibit toxicity against microorganisms when their concentration exceeds several millimolar. An absorption detection system using a quartz crystal microbalance (QCM) was developed to monitor the LCFA concentration during an anaerobic digester’s operation treating oily organic waste. The dissociation of the LCFAs considerably improved the sensor response and, moreover, enabled it to specifically detect LCFA from the mixture of LCFA and triglyceride. Under alkaline conditions, the frequency-shift rates of the QCM sensor linearly increased in accordance with palmitic acid concentration in the range of 0–100 mg/L. Frequency changes caused by anaerobic digestate samples were successfully measured after removing suspended solids and adjusting the pH to 10.7. Finally, the QCM measurements for digestate samples demonstrated that frequency-shift rates are highly correlated with LCFA concentrations, which confirmed that the newly developed QCM sensor is helpful for LCFA monitoring in terms of rapidness and usability.
Understanding Inertial Response of Variable-Speed Wind Turbines by Defined Internal Potential Vector
Authors: Shang Lei , Hu Jiabing , Yuan Xiaoming , Chi Yongning
Published: December 2016

With the rapid development of wind power generation, the inertial response of wind turbines (WTs) has become a topic of wide concern recently, due to its influence on grid frequency dynamics and stability. This paper proposes and defines the inner potential to summarize and understand the inertia control methods and inertial response of type-3 and type-4 WTs, which is analogous to typical synchronous generators (SGs), to make it more easy to understand by system operators and engineers with a traditional power system background. The dynamics of the defined inner potential of the wind turbine without any inertia control is different from SGs, thus the electromechanical inertia is completely hidden. The rapid power control loop and synchronization control loop are the major reasons that the WT’s inertial response is disenabled. On the basis of the defined inner potential’s dynamic, the existing inertia control method for WTs are reviewed and summarized as three approaches, i.e., optimizing the power control or synchronization control or both. At last, the main challenges and issues of these inertia controls are attempted to explain and address.
A Transient Fault Recognition Method for an AC-DC Hybrid Transmission System Based on MMC Information Fusion
Authors: Chen Jikai , Dou Yanhui , Li Yang , Li Jiang , Li Guoqing
Published: December 2016

At present, the research is still in the primary stage in the process of fault disturbance energy transfer in the multilevel modular converter based high voltage direct current (HVDC-MMC). An urgent problem is how to extract and analyze the fault features hidden in MMC electrical information in further studies on the HVDC system. Aiming at the above, this article analyzes the influence of AC transient disturbance on electrical signals of MMC. At the same time, it is found that the energy distribution of electrical signals in MMC is different for different arms in the same frequency bands after the discrete wavelet packet transformation (DWPT). Renyi wavelet packet energy entropy (RWPEE) and Renyi wavelet packet time entropy (RWPTE) are proposed and applied to AC transient fault feature extraction from electrical signals in MMC. Using the feature extraction results of Renyi wavelet packet entropy (RWPE), a novel recognition method is put forward to recognize AC transient faults using the information fusion technology. Theoretical analysis and experimental results show that the proposed method is available to recognize transient AC faults.
Analysis of Micronized Charcoal for Use in a Liquid Fuel Slurry
Authors: Long John , Boyette Michael
Published: December 2016

Yellow poplar (Liriodendron tulipifera) was chosen as the woody biomass for the production of charcoal for use in a liquid fuel slurry. Charcoal produced from this biomass resulted in a highly porous structure similar to the parent material. Micronized particles were produced from this charcoal using a multi-step milling process and verified using a scanning electron microscope and laser diffraction system. Charcoal particles greater than 50 µm exhibited long needle shapes much like the parent biomass while particles less than 50 µm were produced with aspect ratios closer to unity. Laser diffraction measurements indicated D10, D50, and D90 values of 4.446 µm, 15.83 µm, and 39.69 µm, respectively. Moisture content, ash content, absolute density, and energy content values were also measured for the charcoal particles produced. Calculated volumetric energy density values for the charcoal particles exceeded the No. 2 diesel fuel that would be displaced in a liquid fuel slurry.
Power Control of Low Frequency AC Transmission Systems Using Cycloconverters with Virtual Synchronous Generator Control
Authors: Pichetjamroen Achara , Ise Toshifumi
Published: December 2016

This paper is focused on the application of a multi-terminal line-commutated converter-type low frequency AC transmission system (MTLF) using a cycloconverter by applying a new power control scheme for multi-terminal operation. With the virtual synchronous generator (VSG) control scheme, the transmitting power among the multi-terminal system can be accomplished without a communication link for frequency synchronization in each terminal. The details of the proposed control scheme are explained in order to understand the advantages of this method. The configuration of a two-phase low frequency AC transmission system (LFAC) is adopted to examine with the proposed control scheme. Simulation results are provided to illustrate the proposed control scheme with respect to the LFAC system’s performance.
Combining Ecosystem Services with Cost-Benefit Analysis for Selection of Green and Grey Infrastructure for Flood Protection in a Cultural Setting
Authors: Vojinovic Zoran , Keerakamolchai Weeraya , Weesakul Sutat , Pudar Ranko , Medina Neiler , Alves Alida
Published: December 2016

The present paper describes a methodological framework that combines ecosystem services (flood protection, education, art/culture, recreation and tourism) with economic analysis for selection of multifunctional measures for flood resilience. The framework includes active stakeholder participation and it consists of the four main components: (1) identification and valuation of ecosystem services pertinent to the project site under various mitigation scenarios, including baseline (pre-mitigation conditions); (2) evaluation of most effective flood mitigation measures through hydrodynamic simulations, and evaluation of economic viability using cost-benefit analysis; (3) selection of measures through consideration of ecosystem services, and solicitation of stakeholders’ inputs; (4) development of the conceptual landscape design. Application of the framework was demonstrated in a case study of Ayutthaya Island, Thailand. Results of our research suggest that taking a holistic perspective of ecosystem services and economic assessments, marshalled through active stakeholder participation, has the potential to achieve more ecologically sustainable and socially acceptable solutions for flood protection in areas with cultural heritage. However, there is still a considerable challenge in taking this framework to a full-scale practical implementation, and this mainly relates to the selection of indicators that can enable proper application of ecosystem services.
Influence of Fuel Load Dynamics on Carbon Emission by Wildfires in the Clay Belt Boreal Landscape
Authors: Terrier Aurélie , Paquette Mathieu , Gauthier Sylvie , Girardin Martin , Pelletier-Bergeron Sylvain , Bergeron Yves
Published: December 2016

Old-growth forests play a decisive role in preserving biodiversity and ecological functions. In an environment frequently disturbed by fire, the importance of old-growth forests as both a carbon stock as well as a source of emissions when burnt is not fully understood. Here, we report on carbon accumulation with time since the last fire (TSF) in the dominant forest types of the Clay Belt region in eastern North America. To do so, we performed a fuel inventory (tree biomass, herbs and shrubs, dead woody debris, and duff loads) along four chronosequences. Carbon emissions by fire through successional stages were simulated using the Canadian Fire Effects Model. Our results show that fuel accumulates with TSF, especially in coniferous forests. Potential carbon emissions were on average 11.9 t·ha−1 and 29.5 t·ha−1 for old-growth and young forests, respectively. In conclusion, maintaining old-growth forests in the Clay Belt landscape not only ensures a sustainable management of the boreal forest, but it also optimizes the carbon storage.
Using REDD+ Policy to Facilitate Climate Adaptation at the Local Level: Synergies and Challenges in Vietnam
Authors: McElwee Pamela , Thi Nguyen Van , Nguyen Dung , Tran Nghi , Le Hue , Nghiem Tuyen , Thi Vu Huong
Published: December 2016

Attention has recently been paid to how REDD+ mitigation policies are integrated into other sectoral policies, particularly those dealing with climate adaptation at the national level. But there is less understanding of how subnational policy and local projects are able to incorporate attention to adaptation; therefore, we use a case study in Vietnam to discuss how REDD+ projects and policies address both concerns of mitigation and adaptation together at subnational levels. Through stakeholder interviews, focus groups, and household surveys in three provinces of Vietnam with REDD+ activities, our research sought to understand if REDD+ policies and projects on the ground acknowledge that climate change is likely to impact forests and forest users; if this knowledge is built into REDD+ policy and activities; how households in forested areas subject to REDD+ policy are vulnerable to climate change; and how REDD+ activities can help or hinder needed adaptations. Our findings indicate that there continues to be a lack of coordination between mitigation and adaptation policies in Vietnam, particularly with regard to REDD+. Policies for forest-based climate mitigation at the national and subnational level, as well as site-based projects, have paid little attention to the adaptation needs of local communities, many of whom are already suffering from noticeable weather changes in their localities, and there is insufficient discussion of how REDD+ activities could facilitate increased resilience. While there were some implicit and coincidental adaptation benefits of some REDD+ activities, most studied projects and policies did not explicitly target their activities to focus on adaptation or resilience, and in at least one case, negative livelihood impacts that have increased household vulnerability to climate change were documented. Key barriers to integration were identified, such as sectoral specialization; a lack of attention in REDD+ projects to livelihoods; and inadequate support for ecosystem-based adaptation.
Purification and Properties of Yeast Proteases Secreted by Wickerhamomyces anomalus 227 and Metschnikovia pulcherrima 446 during Growth in a White Grape Juice
Authors: Schlander Martina , Distler Ute , Tenzer Stefan , Thines Eckhard , Claus Harald
Published: December 2016

Aspartic proteases are of significant importance for medicine and biotechnology. In spite of sufficient evidence that many non-Saccharomyces yeasts produce extracellular proteases, previous research has focused on the enzymes of Candida species because of their role as virulence factors. Nowadays, there is also increasing interest for their applications in industrial processes, mainly because of their activities at low pH values. Here, we report the features of new acid proteases isolated from wine-relevant yeasts Metschnikovia pulcherrima and Wickerhamomyces anomalus. To our knowledge, this is the first detailed description of such an enzyme derived from strains of W. anomalus. Deviating to most former studies, we could demonstrate that the yeasts produce these enzymes in a natural substrate (grape juice) during the active growth phase. The enzymes were purified from concentrated grape juice by preparative isoelectric focusing. Biochemical data (maximum activity at ≈ pH 3.0, inhibition by pepstatin A) classify them as aspartic proteases. For W. anomalus 227, this assumption was confirmed by the protein sequence of WaAPR1 determined by LC-MS/MS. The sequence revealed a signal peptide for secretion, as well as a peptidase A1 domain with two aspartate residues in the active site. The enzyme has a calculated molecular mass of 47 kDa and an isolelectric point of 4.11.
Parameters for Successful Parental RNAi as An Insect Pest Management Tool in Western Corn Rootworm, Diabrotica virgifera virgifera
Authors: Vélez Ana , Fishilevich Elane , Matz Natalie , Storer Nicholas , Narva Kenneth , Siegfried Blair
Published: December 2016

Parental RNAi (pRNAi) is an RNA interference response where the gene knockdown phenotype is observed in the progeny of the treated organism. pRNAi has been demonstrated in female western corn rootworms (WCR) via diet applications and has been described as a potential approach for rootworm pest management. However, it is not clear if plant-expressed pRNAi can provide effective control of next generation WCR larvae in the field. In this study, we evaluated parameters required to generate a successful pRNAi response in WCR for the genes brahma and hunchback. The parameters tested included a concentration response, duration of the dsRNA exposure, timing of the dsRNA exposure with respect to the mating status in WCR females, and the effects of pRNAi on males. Results indicate that all of the above parameters affect the strength of pRNAi phenotype in females. Results are interpreted in terms of how this technology will perform in the field and the potential role for pRNAi in pest and resistance management strategies. More broadly, the described approaches enable examination of the dynamics of RNAi response in insects beyond pRNAi and crop pests.
Non-Coding RNAs in Lung Cancer: Contribution of Bioinformatics Analysis to the Development of Non-Invasive Diagnostic Tools
Authors: Kunz Meik , Wolf Beat , Schulze Harald , Atlan David , Walles Thorsten , Walles Heike , Dandekar Thomas
Published: December 2016

Lung cancer is currently the leading cause of cancer related mortality due to late diagnosis and limited treatment intervention. Non-coding RNAs are not translated into proteins and have emerged as fundamental regulators of gene expression. Recent studies reported that microRNAs and long non-coding RNAs are involved in lung cancer development and progression. Moreover, they appear as new promising non-invasive biomarkers for early lung cancer diagnosis. Here, we highlight their potential as biomarker in lung cancer and present how bioinformatics can contribute to the development of non-invasive diagnostic tools. For this, we discuss several bioinformatics algorithms and software tools for a comprehensive understanding and functional characterization of microRNAs and long non-coding RNAs.
Mec1/ATR, the Program Manager of Nucleic Acids Inc.
Authors: Feng Wenyi
Published: December 2016

Eukaryotic cells are equipped with surveillance mechanisms called checkpoints to ensure proper execution of cell cycle events. Among these are the checkpoints that detect DNA damage or replication perturbations and coordinate cellular activities to maintain genome stability. At the forefront of damage sensing is an evolutionarily conserved molecule, known respectively in budding yeast and humans as Mec1 (Mitosis entry checkpoint 1) and ATR (Ataxia telangiectasia and Rad3-related protein). Through phosphorylation, Mec1/ATR activates downstream components of a signaling cascade to maintain nucleotide pool balance, protect replication fork integrity, regulate activation of origins of replication, coordinate DNA repair, and implement cell cycle delay. This list of functions continues to expand as studies have revealed that Mec1/ATR modularly interacts with various protein molecules in response to different cellular cues. Among these newly assigned functions is the regulation of RNA metabolism during checkpoint activation and the coordination of replication–transcription conflicts. In this review, I will highlight some of these new functions of Mec1/ATR with a focus on the yeast model organism.
Genetic Regulation of Virulence and Antibiotic Resistance in Acinetobacter baumannii
Authors: Kröger Carsten , Kary Stefani , Schauer Kristina , Cameron Andrew
Published: December 2016

Multidrug resistant microorganisms are forecast to become the single biggest challenge to medical care in the 21st century. Over the last decades, members of the genus Acinetobacter have emerged as bacterial opportunistic pathogens, in particular as challenging nosocomial pathogens because of the rapid evolution of antimicrobial resistances. Although we lack fundamental biological insight into virulence mechanisms, an increasing number of researchers are working to identify virulence factors and to study antibiotic resistance. Here, we review current knowledge regarding the regulation of virulence genes and antibiotic resistance in Acinetobacter baumannii. A survey of the two-component systems AdeRS, BaeSR, GacSA and PmrAB explains how each contributes to antibiotic resistance and virulence gene expression, while BfmRS regulates cell envelope structures important for pathogen persistence. A. baumannii uses the transcription factors Fur and Zur to sense iron or zinc depletion and upregulate genes for metal scavenging as a critical survival tool in an animal host. Quorum sensing, nucleoid-associated proteins, and non-classical transcription factors such as AtfA and small regulatory RNAs are discussed in the context of virulence and antibiotic resistance.
Integrated Care for People with Dementia—Results of a Social-Scientific Evaluation of an Established Dementia Care Model
Authors: Richter Stefanie
Published: December 2016

Currently, approximately 46.8 million people worldwide and 1.47 million German people are affected by dementia. The rising numbers of cases of people with dementia, the need for complex care and the insufficient care available call for innovative and sustainable solutions both in Germany and many other countries. This article presents results of the social-scientific evaluation of an established care model for people with dementia developed by the professionals as a result of acute problems in care in north-east Germany. In addition to the central elements of the model, the conditions of intersectoral and interprofessional cooperation as well as the qualification profile requirements of the professional groups involved are presented in detail. The results can give suggestions for the organization of integrated care for people with dementia in other countries. Further, the author would hereby like to highlight the gain from the scientific examination of solutions to problems in the field.
Legionella Risk Management and Control in Potable Water Systems: Argument for the Abolishment of Routine Testing
Authors: Whiley Harriet
Published: December 2016

Legionella is an opportunistic pathogen of public health significance. One of the main sources of Legionella is potable water systems. As a consequence of aging populations there is an increasing demographic considered at high risk for Legionellosis and, as such, a review of the guidelines is required. Worldwide, Legionella has been detected from many potable water sources, suggesting it is ubiquitous in this environment. Previous studies have identified the limitations of the current standard method for Legionella detection and the high possibility of it returning both false negative and false positive results. There is also huge variability in Legionella test results for the same water sample when conducted at different laboratories. However, many guidelines still recommend the testing of water systems. This commentary argues for the removal of routine Legionella monitoring from all water distribution guidelines. This procedure is financially consuming and false negatives may result in managers being over-confident with a system or a control mechanism. Instead, the presence of the pathogen should be assumed and focus spent on managing appropriate control measures and protecting high-risk population groups.
Changing Diet Quality in China during 2004–2011
Authors: Huang Yingying , Wang Hui , Tian Xu
Published: December 2016

Currently, under- and over-nutrition problems co-exist in China. However, systematic studies on the diet quality of Chinese residents have been scant. This study described the trend in diet quality of Chinese residents over a recent eight-year period and investigated the relevant influential factors. The data of Chinese adults aged 20–59 years was extracted from 2004, 2006, 2009, and 2011 China Health and Nutrition Survey. The China diet quality index (DQI) was employed to assess the diet quality of Chinese adults. The dietary consumption data of each individual was collected using a 24-h dietary recall and weighed food records implemented for three consecutive days. A mixed ordinary least squares regression model was applied to analyze the factors influencing the DQI scores of Chinese residents. Results showed that the diet quality of Chinese residents increased from 2004 to 2006, followed by a decrease in 2009 and 2011. The income, urbanicity index, and southern dummy were positively associated with DQI scores, whereas the size of household and labor intensity were negative predictors of DQI scores. The DQI scores also varied over BMI values. With an increase of the average income level in the future, the diet quality of Chinese residents is estimated to further improve. Moreover, urbanization could also contribute to reaching a more balanced diet.
Efficiency of Emission Control Measures on Particulate Matter-Related Health Impacts and Economic Cost during the 2014 Asia-Pacific Economic Cooperation Meeting in Beijing
Authors: Liu Qichen , Huang Jing , Guo Bin , Guo Xinbiao
Published: December 2016

Background: The Asia-Pacific Economic Cooperation (APEC) meeting was held from 5 November to 11 November 2014 in Beijing, and comprehensive emission control measures were implemented. The efficiency of these measures on particulate matter-related health impacts and economic cost need to be evaluated. Methods: The influences of emission control measures during APEC on particulate matter were evaluated, and health economic effects were assessed. Results: Average concentrations of PM2.5 and PM10 during APEC were reduced by 57.0%, and 50.6% respectively, compared with pre-APEC period. However, the concentrations of particulate matter rebounded after APEC. Compared with the pre-APEC and post-APEC periods, the estimated number of deaths caused by non-accidental, cardiovascular and respiratory diseases that could be attributed to PM2.5 and PM10 during the APEC were the lowest. The economic cost associated with mortality caused by PM2.5 and PM10 during the APEC were reduced by (61.3% and 66.6%) and (50.3% and 60.8%) respectively, compared with pre-APEC and post-APEC. Conclusions: The emission control measures were effective in improving short term air quality and reducing health risks and medical expenses during 2014 APEC, but more efforts is needed for long term and continuous air quality improvement and health protection.
Developmental Neurotoxicity of Methamidophos in the Embryo-Larval Stages of Zebrafish
Authors: He Xiaowei , Gao Jiawei , Dong Tianyu , Chen Minjian , Zhou Kun , Chang Chunxin , Luo Jia , Wang Chao , Wang Shoulin , Chen Daozhen , Zhou Zuomin , Tian Ying , Xia Yankai , Wang Xinru
Published: December 2016

Methamidophos is a representative organophosphate insecticide. The knowledge of its developmental neurotoxicity is limited, especially for zebrafish in the early stages of their life. Four hour post-fertilization (hpf) zebrafish embryos were exposed to several environmentally relevant concentrations of methamidophos (0, 25, and 500 μg/L) for up to 72 hpf. Locomotor behavior was then studied in the zebrafish larvae at this timepoint. Acridine orange (AO) staining was carried out in the zebrafish larvae, and the mRNA levels of genes associated with neural development (mbp and syn2a) were analyzed by reverse transcription-polymerase chain reaction (RT-PCR). The number of escape responders for mechanical stimulation was significantly decreased in exposed groups. AO staining showed noticeable signs of apoptosis mainly in the brain. In addition, the mRNA levels of mbp and syn2a were both significantly down-regulated in exposed groups. Our study provides the first evidence that methamidophos exposure can cause developmental neurotoxicity in the early stages of zebrafish life, which may be caused by the effect of methamidophos on neurodevelopmental genes and the activation of cell apoptosis in the brain.
Prevention of Community-Acquired Pneumonia with Available Pneumococcal Vaccines
Authors: Principi Nicola , Esposito Susanna
Published: December 2016

Community-acquired pneumonia (CAP) places a considerable burden on society. A substantial number of pediatric and adult CAP cases are due to Streptococcus pneumoniae, but fortunately there are effective vaccines available that have a significant impact on CAP-related medical, social, and economic problems. The main aim of this paper is to evaluate the published evidence concerning the impact of pneumococcal vaccines on the prevention of CAP in children and adults. Available data indicate that pneumococcal conjugate vaccines (PCVs) are effective in children, reducing all-cause CAP cases and bacteremic and nonbacteremic CAP cases. Moreover, at least for PCV7 and PCV13, vaccination of children is effective in reducing the incidence of CAP among adults. Recently use of PCV13 in adults alone or in combination with the pneumococcal polysaccharide vaccine has been suggested and further studies can better define its effectiveness in this group of subjects. The only relevant problem for PCV13 is the risk of a second replacement phenomenon, which might significantly reduce its real efficacy in clinical practice. Protein-based pneumococcal vaccines might be a possible solution to this problem.
Troxerutin Attenuates Enhancement of Hepatic Gluconeogenesis by Inhibiting NOD Activation-Mediated Inflammation in High-Fat Diet-Treated Mice
Authors: Zhang Zifeng , Wang Xin , Zheng Guihong , Shan Qun , Lu Jun , Fan Shaohua , Sun Chunhui , Wu Dongmei , Zhang Cheng , Su Weitong , Sui Junwen , Zheng Yuanlin
Published: December 2016

Recent evidence suggests that troxerutin, a trihydroxyethylated derivative of natural bioflavonoid rutin, exhibits beneficial effects on diabetes-related symptoms. Here we investigated the effects of troxerutin on the enhancement of hepatic gluconeogenesis in high-fat diet (HFD)-treated mice and the mechanisms underlying these effects. Mice were divided into four groups: Control group, HFD group, HFD + Troxerutin group, and Troxerutin group. Troxerutin was treated by daily oral administration at doses of 150 mg/kg/day for 20 weeks. Tauroursodeoxycholic acid (TUDCA) was used to inhibit endoplasmic reticulum stress (ER stress). Our results showed that troxerutin effectively improved obesity and related metabolic parameters, and liver injuries in HFD-treated mouse. Furthermore, troxerutin significantly attenuated enhancement of hepatic gluconeogenesis in HFD-fed mouse. Moreover, troxerutin notably suppressed nuclear factor-κB (NF-κB) p65 transcriptional activation and release of inflammatory cytokines in HFD-treated mouse livers. Mechanismly, troxerutin dramatically decreased Nucleotide oligomerization domain (NOD) expression, as well as interaction between NOD1/2 with interacting protein-2 (RIP2), by abating oxidative stress-induced ER stress in HFD-treated mouse livers, which was confirmed by TUDCA treatment. These improvement effects of troxerutin on hepatic glucose disorders might be mediated by its anti-obesity effect. In conclusion, troxerutin markedly diminished HFD-induced enhancement of hepatic gluconeogenesis via its inhibitory effects on ER stress-mediated NOD activation and consequent inflammation, which might be mediated by its anti-obesity effect.
In Vivo Assessment of Clobetasol Propionate-Loaded Lecithin-Chitosan Nanoparticles for Skin Delivery
Authors: Şenyiğit Taner , Sonvico Fabio , Rossi Alessandra , Tekmen Işıl , Santi Patrizia , Colombo Paolo , Nicoli Sara , Özer Özgen
Published: December 2016

The aim of this work was to assess in vivo the anti-inflammatory efficacy and tolerability of clobetasol propionate (CP) loaded lecithin/chitosan nanoparticles incorporated into chitosan gel for topical application (CP 0.005%). As a comparison, a commercial cream (CP 0.05% w/w), and a sodium deoxycholate gel (CP 0.05% w/w) were also evaluated. Lecithin/chitosan nanoparticles were prepared by self-assembling of the components obtained by direct injection of soybean lecithin alcoholic solution containing CP into chitosan aqueous solution. Nanoparticles obtained had a particle size around 250 nm, narrow distribution (polydispersity index below 0.2) and positive surface charge, provided by a superficial layer of the cationic polymer. The nanoparticle suspension was then loaded into a chitosan gel, to obtain a final CP concentration of 0.005%. The anti-inflammatory activity was evaluated using carrageenan-induced hind paw edema test on Wistar rats, the effect of formulations on the barrier property of the stratum corneum were determined using transepidermal water loss measurements (TEWL) and histological analysis was performed to evaluate the possible presence of morphological changes. The results obtained indicate that nanoparticle-in-gel formulation produced significantly higher edema inhibition compared to other formulations tested, although it contained ten times less CP. TEWL measurements also revealed that all formulations have no significant disturbance on the barrier function of skin. Furthermore, histological analysis of rat abdominal skin did not show morphological tissue changes nor cell infiltration signs after application of the formulations. Taken together, the present data show that the use of lecithin/chitosan nanoparticles in chitosan gel as a drug carrier significantly improves the risk-benefit ratio as compared with sodium-deoxycholate gel and commercial cream formulations of CP.
Changes of Photosynthetic Behaviors and Photoprotection during Cell Transformation and Astaxanthin Accumulation in Haematococcus pluvialis Grown Outdoors in Tubular Photobioreactors
Authors: Zhang Litao , Su Fang , Zhang Chunhui , Gong Fengying , Liu Jianguo
Published: December 2016

The cell transformation from green motile cells to non-motile cells and astaxanthin accumulation can be induced in the green alga Haematococcus pluvialis cultured outdoors. In the initial 3 d of incubation (cell transformation phase), light absorption and photosynthetic electron transport became more efficient. After five days of incubation (astaxanthin accumulation phase), the light absorption per active reaction center (ABS/RC) increased, but the efficiency of electron transport (ψo) and the quantum yield of electron transport (φEo) decreased with increased time, indicating that the capacity of photosynthetic energy utilization decreased significantly during astaxanthin accumulation, leading to an imbalance between photosynthetic light absorption and energy utilization. It would inevitably aggravate photoinhibition under high light, e.g., at midday. However, the level of photoinhibition in H. pluvialis decreased as the incubation time increased, which is reflected by the fact that Fv/Fm determined at midday decreased significantly in the initial 3 d of incubation, but was affected very little after seven days of incubation, compared with that determined at predawn. This might be because the non-photochemical quenching, plastid terminal oxidase, photosystem I cyclic electron transport, defensive enzymes and the accumulated astaxanthin can protect cells against photoinhibition.
Lifetime Increased Risk of Adult Onset Atopic Dermatitis in Adolescent and Adult Patients with Food Allergy
Authors: Yu Hsu-Sheng , Tu Hung-Pin , Hong Chien-Hui , Lee Chih-Hung
Published: December 2016

Food allergy can result in life-threatening anaphylaxis. Atopic dermatitis (AD) causes intense itching and impaired quality of life. Previous studies have shown that patients with classical early-onset AD tend to develop food allergy and that 10% of adults with food allergies have concomitant AD. However, it is not known whether late-onset food allergy leads to adult-onset AD, a recently recognized disease entity. Using an initial cohort of one-million subjects, this study retrospectively followed-up 2851 patients with food allergy (age > 12 years) for 14 years and compared them with 11,404 matched controls. While 2.8% (81) of the 2851 food allergy patients developed AD, only 2.0% (227) of the 11,404 controls developed AD. Multivariate regression analysis showed that food allergy patients were more likely to develop AD (adjusted hazard ratio = 2.49, p < 0.0001). Controls had a 1.99% risk of developing AD, while food allergy patients had a significantly higher risk (7.18% and 3.46% for patients with ≥3 and <3 food allergy claims, respectively) of developing adult-onset AD. This is the first study to describe the chronological and dose-dependent associations between food allergy in adolescence and the development of adult-onset AD.
Subcellular Trafficking of Mammalian Lysosomal Proteins: An Extended View
Authors: Staudt Catherine , Puissant Emeline , Boonen Marielle
Published: December 2016

Lysosomes clear macromolecules, maintain nutrient and cholesterol homeostasis, participate in tissue repair, and in many other cellular functions. To assume these tasks, lysosomes rely on their large arsenal of acid hydrolases, transmembrane proteins and membrane-associated proteins. It is therefore imperative that, post-synthesis, these proteins are specifically recognized as lysosomal components and are correctly sorted to this organelle through the endosomes. Lysosomal transmembrane proteins contain consensus motifs in their cytosolic regions (tyrosine- or dileucine-based) that serve as sorting signals to the endosomes, whereas most lysosomal acid hydrolases acquire mannose 6-phosphate (Man-6-P) moieties that mediate binding to two membrane receptors with endosomal sorting motifs in their cytosolic tails. These tyrosine- and dileucine-based motifs are tickets for boarding in clathrin-coated carriers that transport their cargo from the trans-Golgi network and plasma membrane to the endosomes. However, increasing evidence points to additional mechanisms participating in the biogenesis of lysosomes. In some cell types, for example, there are alternatives to the Man-6-P receptors for the transport of some acid hydrolases. In addition, several “non-consensus” sorting motifs have been identified, and atypical transport routes to endolysosomes have been brought to light. These “unconventional” or “less known” transport mechanisms are the focus of this review.
Lymph Nodes and Cancer Metastasis: New Perspectives on the Role of Intranodal Lymphatic Sinuses
Authors: Ji Rui-Cheng
Published: December 2016

The lymphatic system is essential for transporting interstitial fluid, soluble antigen, and immune cells from peripheral tissues to lymph nodes (LNs). Functional integrity of LNs is dependent on intact lymphatics and effective lymph drainage. Molecular mechanisms that facilitate interactions between tumor cells and lymphatic endothelial cells (LECs) during tumor progression still remain to be identified. The cellular and molecular structures of LNs are optimized to trigger a rapid and efficient immune response, and to participate in the process of tumor metastasis by stimulating lymphangiogenesis and establishing a premetastatic niche in LNs. Several molecules, e.g., S1P, CCR7-CCL19/CCL21, CXCL12/CXCR4, IL-7, IFN-γ, TGF-β, and integrin α4β1 play an important role in controlling the activity of LN stromal cells including LECs, fibroblastic reticular cells (FRCs) and follicular dendritic cells (DCs). The functional stromal cells are critical for reconstruction and remodeling of the LN that creates a unique microenvironment of tumor cells and LECs for cancer metastasis. LN metastasis is a major determinant for the prognosis of most human cancers and clinical management. Ongoing work to elucidate the function and molecular regulation of LN lymphatic sinuses will provide insight into cancer development mechanisms and improve therapeutic approaches for human malignancy.
Aquaporins in Brain Edema and Neuropathological Conditions
Authors: Filippidis Aristotelis , Carozza Richard , Rekate Harold
Published: December 2016

The aquaporin (AQP) family of water channels are a group of small, membrane-spanning proteins that are vital for the rapid transport of water across the plasma membrane. These proteins are widely expressed, from tissues such as the renal epithelium and erythrocytes to the various cells of the central nervous system. This review will elucidate the basic structure and distribution of aquaporins and discuss the role of aquaporins in various neuropathologies. AQP1 and AQP4, the two primary aquaporin molecules of the central nervous system, regulate brain water and CSF movement and contribute to cytotoxic and vasogenic edema, where they control the size of the intracellular and extracellular fluid volumes, respectively. AQP4 expression is vital to the cellular migration and angiogenesis at the heart of tumor growth; AQP4 is central to dysfunctions in glutamate metabolism, synaptogenesis, and memory consolidation; and AQP1 and AQP4 adaptations have been seen in obstructive and non-obstructive hydrocephalus and may be therapeutic targets.
Next Forwarding Node Selection in Underwater Wireless Sensor Networks (UWSNs): Techniques and Challenges
Authors: Khasawneh Ahmad , Latiff Muhammad , Kaiwartya Omprakash , Chizari Hassan
Published: December 2016

The underwater wireless sensor network (UWSN) is considered a promising technology for collecting valuable data from underwater areas, particularly for aiding military operations and environmental predictions. UWSNs consist of underwater sensor nodes that have limited energy and use acoustics for communication. Routing in underwater sensor nodes is one of the challenging issues in UWSNs because of the need to forward data packets with minimal energy consumption and a high packet delivery ratio. Selecting the next forwarding nodes is one of the key components of routing in UWSNs and has a direct effect on energy consumption and the packet delivery ratio. Therefore, this problem has gained much attention from the research community with the intent of enhancing the performance of UWSNs. This paper qualitatively reviews routing protocols for UWSNs, focusing on the next-hop selection method and its strengths and weaknesses. A taxonomy is presented for reviewing routing protocols under different categories of the classification. A summary of the qualitative investigation is presented highlighting aims, the next-hop selection method, metrics, and priority considerations. A comprehensive investigation is carried out focusing on energy, link quality, void awareness, reliability, and shortest path characteristics. Finally, we discuss potential future research directions in UWSNs for forwarding node selection.
Childhood Cognitive Ability Predicts Adult Financial Well-Being
Authors: Furnham Adrian , Cheng Helen
Published: December 2016

This study set out to investigate to what extent childhood cognitive ability, along with personality traits, education and occupational status, as well as marital status influence adult financial success. Data were drawn from a large, prospective birth cohort in the UK, the National Child Development Study (NCDS). The analytic sample was comprised of 4537 cohort members with data on parental social class (at birth), cognitive ability (at age 11), educational qualifications (at age 33), personality traits (at age 50), current marital status and occupational prestige, and salary/wage earning level (all measured at age 54). Correlational results showed that parental social class, childhood cognitive ability, traits extraversion, emotional stability, conscientiousness, and openness, being married positively, being divorced or separated negatively, education and occupation as well as gender were all significantly associated with adult earning ability (p < 0.05 to p < 0.001). Effect sizes for the relationship between intelligence and income was moderate. Results of a multiple regression analysis showed that childhood cognitive ability, traits conscientiousness and openness, educational qualifications and occupational prestige were significant and independent predictors of adult earning ability accounting for 30% of the total variance. There was also a gender effect on the outcome variable. Numerous limitations are noted.