bluuebunny/crossref_metadata_2025 huggingface dataset:
Macrophage-driven cardiac inflammation and healing: insights from homeostasis and myocardial infarction
Authors: Zuo Wenjie , Sun Renhua , Ji Zhenjun , Ma Genshan
Published: October 2023

Early and prompt reperfusion therapy has markedly improved the survival rates among patients enduring myocardial infarction (MI). Nonetheless, the resulting adverse remodeling and the subsequent onset of heart failure remain formidable clinical management challenges and represent a primary cause of disability in MI patients worldwide. Macrophages play a crucial role in immune system regulation and wield a profound influence over the inflammatory repair process following MI, thereby dictating the degree of myocardial injury and the subsequent pathological remodeling. Despite numerous previous biological studies that established the classical polarization model for macrophages, classifying them as either M1 pro-inflammatory or M2 pro-reparative macrophages, this simplistic categorization falls short of meeting the precision medicine standards, hindering the translational advancement of clinical research. Recently, advances in single-cell sequencing technology have facilitated a more profound exploration of macrophage heterogeneity and plasticity, opening avenues for the development of targeted interventions to address macrophage-related factors in the aftermath of MI. In this review, we provide a summary of macrophage origins, tissue distribution, classification, and surface markers. Furthermore, we delve into the multifaceted roles of macrophages in maintaining cardiac homeostasis and regulating inflammation during the post-MI period.
Cross-tissue patterns of DNA hypomethylation reveal genetically distinct histories of cell development
Authors: Scott Timothy J. , Hansen Tyler J. , McArthur Evonne , Hodges Emily
Published: October 2023

Background

Establishment of DNA methylation (DNAme) patterns is essential for balanced multi-lineage cellular differentiation, but exactly how these patterns drive cellular phenotypes is unclear. While > 80% of CpG sites are stably methylated, tens of thousands of discrete CpG loci form hypomethylated regions (HMRs). Because they lack DNAme, HMRs are considered transcriptionally permissive, but not all HMRs actively regulate genes. Unlike promoter HMRs, a subset of non-coding HMRs is cell type-specific and enriched for tissue-specific gene regulatory functions. Our data further argues not only that HMR establishment is an important step in enforcing cell identity, but also that cross-cell type and spatial HMR patterns are functionally informative of gene regulation.

Results

To understand the significance of non-coding HMRs, we systematically dissected HMR patterns across diverse human cell types and developmental timepoints, including embryonic, fetal, and adult tissues. Unsupervised clustering of 126,104 distinct HMRs revealed that levels of HMR specificity reflects a developmental hierarchy supported by enrichment of stage-specific transcription factors and gene ontologies. Using a pseudo-time course of development from embryonic stem cells to adult stem and mature hematopoietic cells, we find that most HMRs observed in differentiated cells (~ 60%) are established at early developmental stages and accumulate as development progresses. HMRs that arise during differentiation frequently (~ 35%) establish near existing HMRs (≤ 6 kb away), leading to the formation of HMR clusters associated with stronger enhancer activity. Using SNP-based partitioned heritability from GWAS summary statistics across diverse traits and clinical lab values, we discovered that genetic contribution to trait heritability is enriched within HMRs. Moreover, the contribution of heritability to cell-relevant traits increases with both increasing HMR specificity and HMR clustering, supporting the role of distinct HMR subsets in regulating normal cell function.

Conclusions

Our results demonstrate that the entire HMR repertoire within a cell-type, rather than just the cell type-specific HMRs, stores information that is key to understanding and predicting cellular phenotypes. Ultimately, these data provide novel insights into how DNA hypo-methylation provides genetically distinct historical records of a cell’s journey through development, highlighting HMRs as functionally distinct from other epigenomic annotations.
The energy metabolism of Balantidium polyvacuolum inhabiting the hindgut of Xenocypris davidi
Authors: Bu Xia-lian , Zhao Wei-shan , Li Zhong-yang , Ma Hong-wei , Chen Yu-shun , Li Wen-xiang , Zou Hong , Li Ming , Wang Gui-tang
Published: October 2023

Anaerobic parasitic ciliates are a specialized group of ciliates that are adapted to anoxic and oxygen-depleted habitats. Among them, Balantidium polyvacuolum, which inhabits the hindgut of Xenocyprinae fishes, has received very limited scientific attention, so the molecular mechanism of its adaptation to the digestive tract microenvironment is still unclear. In this study, transmission electron microscopy (TEM) and single-cell transcriptome analysis were used to uncover the metabolism of B. polyvacuolum. Starch granules, endosymbiotic bacteria, and multiple specialized mitochondrion-related organelles (MROs) of various shapes were observed. The MROs may have completely lost the electron transport chain (ETC) complexes I, III, IV, and V and only retained succinate dehydrogenase subunit A (SDHA) of complex II. The tricarboxylic acid (TCA) cycle was also incomplete. It can be inferred that the hypoxic intestinal environment has led to the specialization of the mitochondria in B. polyvacuolum. Moreover, carbohydrate-active enzymes (CAZymes), including carbohydrate esterases, enzymes with a carbohydrate-binding module, glycoside hydrolases, and glycosyltransferases, were identified, which may constitute evidence that B. polyvacuolum is able to digest carbohydrates and starch. These findings can improve our knowledge of the energy metabolism and adaptive mechanisms of B. polyvacuolum.
Online commercial sex-seeking among female sex workers in south China: a cross-sectional study
Authors: Zhao Peizhen , Xu Wenqian , Ye Rouxuan , Shi Yijia , Wang Cheng
Published: October 2023

Background

Online communication platforms have the potential to facilitate commercial sex among female sex workers (FSW), increasing the risk of contracting sexually transmitted diseases (STD). This study aimed to describe the patterns of online commercial sex-seeking and examine the associated factors among FSW in China.

Methods

A venue-based cross-sectional study was conducted in five cities in Guangdong Province, China, between April and October 2020. Data on socio-demographic characteristics, sexual behaviors, and online commercial sex-seeking patterns were collected through face-to-face interviews. Venous blood and urine samples were collected for STD testing. Univariate and multivariable logistic regressions were used to explore the factors associated with online commercial sex-seeking.

Results

A total of 1155 FSW were recruited in physical venues for this study. Among them, 33.42% reported ever using online applications to seek commercial sex. The prevalence of HIV, syphilis, gonorrhea, chlamydia, and STD was 0.26%, 1.30%, 4.40%, 15.54%, and 18.39%, respectively, among FSW who had ever used both physical and online venues to seek commercial sex, which was higher than among FSW who had never sought commercial sex online. Multivariable logistic regression indicated that FSW who used online platforms to seek commercial sex were more likely to have STD (adjusted odds ratio (aOR) = 1.48, 95%CI: 1.05–2.09), experience unintended pregnancies due to commercial sex (aOR = 1.78, 95%CI: 1.21–2.62), be diagnosed as infertile (aOR = 3.20, 95%CI: 1.42–7.21), and undergo abortions (aOR = 1.69, 95%CI: 1.29–2.20).

Conclusion

A significant proportion of FSW who practiced in physical venues in China engaged in seeking commercial sex online, and this behavior is positively correlated with both STD and reproductive health outcomes. Given the high prevalence of online sex-seeking, it is crucial to provide a wide range of internet-based healthcare interventions and reproductive health services to Chinese FSW.
Upregulated CANT1 is correlated with poor prognosis in hepatocellular carcinoma
Authors: Liu Ting , Li Zhi-zhao , Sun Lei , Yang Kun , Chen Jia-min , Han Xiao-yi , Qi Li-ming , Zhou Xin-gang , Wang Peng
Published: October 2023

Background

CANT1, as calcium-activated protein nucleotidase 1, is a kind of phosphatase. It is overexpressed in some tumors and related to poor prognosis, but few studies explore its function and carcinogenic mechanism in hepatocellular carcinoma (HCC).

Methods

The expression of CANT1 mRNA and protein was analyzed by the Cancer Genome Atlas (TCGA) database and immunohistochemistry(IHC) staining. The relationship between CANT1 expression and clinicopathology was evaluated by various public databases. The receiver operating characteristic (ROC) curve was used to assess the diagnostic accuracy of CANT1 by the area under curve (AUC). Univariate, multivariate Cox regression and Kaplan-Meier curves were applied to evaluate the predictive value of CANT1 on the prognosis of HCC. Methsurv was used to analyze gene changes and DNA methylation, and its impact on prognosis. The enrichment analysis of DEGs associated with CANT1 revealed the biological process of CANT1 based on Gene Set Enrichment Analysis (GSEA). The relationship between immune cell infiltration level and CANT1 expression in HCC was investigated using the single-sample GSEA (ssGSEA) method and the Tumor Immune Estimation Resource (TIMER) database. Finally, the association between CANT1 and immune checkpoints and drug sensitivity was also analyzed.

Results

CANT1 was highly expressed in 22 cancers, including HCC, and CANT1 overexpression in HCC was confirmed by IHC. The expression of CANT1 was correlated with clinical features, such as histologic grade. Highly expressed CANT1 caused poor overall survival (OS) of HCC patients. Univariate and multivariate regression analysis suggested that CANT1 was an independent prognostic marker. Of the 31 DNA methylation at CpG sites, three CpG sites were associated with the prognosis of HCC. GSEA indicated that CANT1 was mainly involved in the cell cycle, DNA replication, and etc. Moreover, CANT1 expression was correlated with immune cell infiltration and independently associated with the prognosis of HCC patients. Finally, CANT1 expression was correlated with most immune checkpoints and drug sensitivity.

Conclusion

CANT1 may be a latent oncogene of HCC, and associated with immune cells and immune checkpoints, which may assist in HCC treatment.
Impact of a publicly-funded pharmacare program policy on benzodiazepine dispensing among children and youth: a population-based natural experiment
Authors: Antoniou Tony , McCormack Daniel , Kitchen Sophie , Pajer Kathleen , Gardner William , Lunsky Yona , Penner Melanie , Tadrous Mina , Mamdani Muhammad , Juurlink David N. , Gomes Tara
Published: October 2023

Background

In January 2018, the Government of Ontario, Canada, initiated a universal pharmacare program (OHIP+) for all individuals aged 24 years and younger. In April 2019, the program was amended to cover only children and youth without private insurance. Because benzodiazepines are commonly prescribed to children and youth despite their potential hazards, we examined whether changes in publicly-funded drug coverage influenced benzodiazepine dispensing trends in this demographic.

Methods

We conducted a population-based natural experiment study of benzodiazepine dispensing to children and youth in Ontario between January 2013 and March 2020. We used interventional autoregressive integrated moving average models to estimate the impact of OHIP + and its subsequent modification on these trends.

Results

The implementation of OHIP + was associated with an immediate increase in the monthly rate of benzodiazepine dispensing of 12.9 individuals per 100,000 population (95% confidence interval [CI]; 7.5 to 18.3 per 100,000). Benzodiazepine dispensing rates rose from 214.2 to 241.5 per 100,000 from December 2017 to March 2019, a 12.8% (95% CI 9.6–16.0%) increase. In stratified analyses, increases were most pronounced among females, children and youth living in the lowest income neighbourhoods and individuals aged 20 to 24. The April 2019 modification to OHIP + was not associated with changes in monthly benzodiazepine dispensing trends (0.39 individuals per 100,000; 95% CI -1.3 to 2.1 per 100,000). However, rates remained elevated relative to the period preceding OHIP + implementation.

Conclusions

Implementation of a publicly-funded pharmacare program resulted in more children and youth being prescribed benzodiazepines.
Parental technoference and adolescents’ mental health and violent behaviour: a scoping review
Authors: Dixon Donna , Sharp Catherine A. , Hughes Karen , Hughes J. Carl.
Published: October 2023

Purpose

The term ‘technoference’ refers to habitual interferences and disruptions within interpersonal relationships or time spent together due to use of electronic devices. Emerging evidence suggests associations between parental technoference and young people’s mental health and violent behaviours. This scoping review sought to summarise the existing literature.

Methods

A scoping review was undertaken across six databases (APA PsycINFO, MEDLINE, ASSIA, ERIC, Social Sciences Premium Collection, SciTech Premium). Searches included articles examining the association between parental technoference and adolescent mental health and violent behaviours. All included studies provided empirical findings.

Results

Searches retrieved 382 articles, of which 13 articles met the eligibility criteria. A narrative approach was applied to synthesise the eligible findings. Across all studies, adolescent perceptions of parental technoference were negatively associated to adolescent mental health and positively related to adolescent violent behaviours. Parental cohesion and mental health were identified as significant mediating factors.

Conclusion

Findings suggest that parents should be aware of the environment in which they use electronic devices as their use can potentially, directly and indirectly, influence adolescent mental health and violent behaviours. Further research into the potential caveats of parental technoference could support the development of evidence-informed guidelines for parental management of electronic devices.
Examining supporting and constraining factors of physicians’ acceptance of telemedical online consultations: a survey study
Authors: Diel Sören , Doctor Eileen , Reith Riccardo , Buck Christoph , Eymann Torsten
Published: October 2023

As healthcare demands exceed outpatient physicians’ capacities, telemedicine holds far-reaching potential for both physicians and patients. It is crucial to holistically analyze physicians’ acceptance of telemedical applications, such as online consultations. This study seeks to identify supporting and constraining factors that influence outpatient physicians’ acceptance of telemedicine.

We develop a model based on the unified theory of acceptance and use of technology (UTAUT). To empirically examine our research model, we conducted a survey among German physicians (n = 127) in 2018–2019. We used the partial least squares (PLS) modeling approach to test our model, including a mediation analysis.

The results indicate that performance expectancy (β = .397, P < .001), effort expectancy (β = .134, P = .03), and social influence (β = .337, P < .001) strongly impact the intention to conduct online consultations and explain 55% of its variance. Structural conditions regarding data security comprise a key antecedent, associating with performance expectancy (β = .193, P < .001) and effort expectancy (β = .295, P < .001). Regarding potential barriers to usage intentions, we find that IT anxiety predicts performance (β = –.342, P < .001) and effort expectancy (β = –.364, P < .001), while performance expectancy fully mediates (βdirect = .022, P = .71; βindirect = -.138, P < .001) the direct relationship between IT anxiety and the intention to use telemedical applications.

This research provides explanations for physicians’ behavioral intention to use online consultations, underlining UTAUT’s applicability in healthcare contexts. To boost acceptance, social influences, such as personal connections and networking are vital, as colleagues can serve as multipliers to reach convergence on online consultations among peers. To overcome physicians’ IT anxiety, training, demonstrations, knowledge sharing, and management incentives are recommended. Furthermore, regulations and standards to build trust in the compliance of online consultations with data protection guidelines need reinforcement from policymakers and hospital management alike.
A mixed methods study of using wasta in healthcare services in Palestine: predictors, consequences and proposed solutions
Authors: Takruri Adel , Nawajah Inad , Jabari Carol El
Published: October 2023

Background

Equity in access to quality healthcare is a fundamental human right. Yet studies demonstrate that some people receive preferential treatment while others are discriminated against. Wasta is a prevalent strategy whereby personal connections are used for influence and may result in gaining unfair advantages over others. This study aims to investigate wasta use in healthcare, the factors associated with its use, and the impact of wasta use on the equity and quality of healthcare services.

Methods

A mixed-methods study utilizing a quantitative survey and qualitative interviews was conducted in Palestine (West Bank and Gaza) between October 2021 and February 2022. Quantitative analysis was performed using Stata version 14. Bivariate and multivariate logistic regressions assessed the relationship between wasta use and individual-level variables such as gender, residence, age, employment status, and financial situation. Content analyses of qualitative transcripts were performed using Dedoose version 9. Textual quotes were grouped into major and minor themes.

Results

Multivariate regressions revealed that wasta use is more prevalent among refugee camp dwellers and more frequent in Gaza compared to the West Bank. Wasta was also employed to a greater degree among government employees. Qualitative interviews complemented the quantitative results and added further insights into the consequences of Wasta use in healthcare settings such as negatively impacting quality and equity in healthcare services.

Conclusion

Wasta use in healthcare can have an adverse effect on equity and quality. Ensuring efficient processes, reduced financial burdens, stringent accountability measures, transparency, and training programs can contribute to diminishing the need for using wasta in healthcare. By addressing both systemic and cultural factors that perpetuate wasta, societies can move closer to healthcare systems characterized by fairness, accessibility, and ethical integrity.
Biochemical markers and FokI and TaqI vitamin D receptor genes polymorphism in rheumatoid arthritis
Authors: Ahmad Hosam M. , Zaki Zaki M. , Mohamed Asmaa S. , Ahmed Amr E.
Published: October 2023

Background

Previous studies have reported the role of genes in different metabolic processes in the human body, and any variation in gene polymorphisms could lead to disturbances in these processes and different diseases.

Objective

This study aimed to compare vitamin D receptor (VDR) FokI and TaqI genotypes in terms of parathyroid hormone (PTH) and some biomarkers of inflammation and susceptibility to rheumatoid arthritis (RA) disease.

Methods

This study included 100 patients with rheumatoid arthritis (RA). Genotyping was performed by polymerase chain reaction (PCR) and examined by specific restriction enzymes using restriction fragment length polymorphism (RFLP). Serum intact PTH, C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), rheumatoid factor (RF), and anti-cyclic citrullinated peptide antibodies (ACCPs) levels were measured.

Results

An increased PTH level (> 65 pg/ml) was found in 8% of patients. No significant differences among FokI and TaqI vitamin D receptor genes polymorphism regarding positive and negative RF or ACCPs were found. A significant difference was found among FokI (p = 0.009) and none in TaqI genotypes regarding intact parathyroid hormone level categories. No significant correlation was found between the serum intact PTH level and ESR or CRP levels (P = 0.13 and 0.28, respectively). The parathyroid hormone level was not a good predictor for RF or ACCPs (P = 0.5 and 0.06, respectively).

Conclusion

The FokI gene may play a role in controlling PTH levels in patients with RA. There was no significant correlation found between the serum intact PTH level and RA severity according to ESR and CRP inflammatory biomarkers. There are no differences between VDR genes FokI and TaqI polymorphism in terms of RA susceptibility (for RF and ACCPs).
Multi-rod posterior correction only with halo-femoral traction for the management of adult neuromuscular scoliosis (> 100°) with severe pelvic obliquity: a minimum 5-year follow-up
Authors: Zhang Hong-Qi , Deng Ang , Guo Chao-Feng , Sun Yang , Li Meng-Jun
Published: October 2023

Background

Many patients with neuromuscular scoliosis (NMS) experience a variety of difficult medical problems that aggravate the development effects of progressive scoliosis and pelvic obliquity (PO). The objective of the current study was to assess the safety and effectiveness of multi-rod posterior correction only (MRPCO) with halo-femoral traction (HFT) for the management of adult NMS (> 100°) with severe PO.

Methods

From 2012 to 2017, 13 adult patients who suffered from NMS (> 100°) with severe PO underwent MRPCO with HFT. The radiography parameters in a sitting position, such as the coronal Cobb angle of the main curve, the PO and the trunk shift (TS), were measured at the preoperative, postoperative and final follow-up stages. The preoperative and final follow-up assessment of the Visual Analogue Scale (VAS) and Oswestry Disability Index (ODI) was taken.

Results

The average follow-up span was 68.15 ± 6.78 months. There was decreased postoperative coronal Cobb angle with an average mean of 125.24° ± 11.78° to 47.55° ± 12.10°, with a correction rate of 62.43%; the PO was reduced to 6.25° ± 1.63° from 36.93° ± 4.25° with a correction rate of 83.07%; the TS was reduced to 2.41 cm ± 1.40 cm from 9.19 cm ± 3.07 cm. There was significant improvement in all parameters compared to the preoperative data. The VAS score reduced from 4.77 ± 0.93 to 0.69 ± 0.75, and the ODI score reduced from 65.38 ± 16.80 to 28.62 ± 12.29 at the final follow-up.

Conclusions

Treatment of adult NMS (> 100°) with severe PO could be safe and effective with MRPCO with HFT. In order to obtain the optimum sitting balance, this could reduce the prevalence of complications and rectify the curvature and the correction of PO.
Cardiometabolic, functional, and psychosocial effects of a remotely supervised home-based exercise program in individuals with type 2 diabetes (RED study): study protocol for a randomized clinical trial
Authors: Rodrigues Samara Nickel , Delevatti Rodrigo Sudatti , Carvalho Mauricio Tatsch Ximenes , Bullo Valentina , Bergamin Marco , Alberton Cristine Lima
Published: October 2023

Background

Type 2 diabetes mellitus (T2D) is a serious global health problem, and exercise is considered an essential non-pharmacological tool in T2D prevention and treatment. During periods of social isolation experienced by the COVID-19 pandemic, home-based exercise programs were strongly recommended as a strategy to facilitate exercise practice and reduce the negative impacts of social isolation. Remotely supervised exercise stands out as an easily accessible strategy after the pandemic, as it is a tool that aims to facilitate access to exercise by this population. The purpose of the RED study is to verify the effects of a remotely supervised home-based exercise program compared to a control group on cardiometabolic, functional, and psychosocial outcomes in patients with T2D.

Methods

Participants are randomized into the control group (CG) and the intervention group (IG). Participants allocated to the CG receive recommendations for the practice of physical activity based on information from chapters of the Physical Activity Guide for the Brazilian Population, while the IG will perform a 12-week home-based exercise program supervised remotely by video call. The intervention has a weekly frequency of two sessions per week on non-consecutive days during the first 6 weeks and three sessions per week on non-consecutive days for the remaining 6 weeks. The RED study has HbA1c as the primary outcome, and the participants’ cardiometabolic, functional, and psychosocial parameters are assessed at baseline (week 0) and post-intervention (week 13).

Discussion

Expected results of the proposed study will provide the knowledge base of health professionals and deliver more evidence for a growing area, i.e., home-based exercise and T2D. Additionally, this protocol aims to verify and demonstrate whether this program can be accessible and effective for different health outcomes in patients with T2D.

Trial registration

The RED study protocol was prospectively registered at ClinicalTrials.gov (NCT05362071). Date registered April 6, 2022. https://clinicaltrials.gov/ct2/show/NCT05362071.
Cryopreservation and validation of differentiated PDGFRα-positive cells for long term usage in experimentation
Authors: Srivastava Ankita , Rajan Reeja , Abdallah Sallam Hasan , Khan Amir Ali , Mussa Bashair M.
Published: October 2023

Objective

Differentiation of immortalized Mesenchymal Stromal Cells (iMSCs) into PDGFRα-positive cells under controlled growth conditions has several vital implications in functional studies concerned with the pathogenesis of Diabetic Gastroparesis (DGP). A study published previously by our research group demonstrated the importance of these cells as a novel, in-vitro model for investigating the functional role of neuronal nitric oxide synthase. The currently available methods require fresh differentiation of PDGFRα-positive cells for each round of experimentation. This leads to longer delays, higher usage of reagents, and inconsistency in reproducibility of experiments frequently. We thus aimed to establish through validation that cryopreserving and maintaining the iMSC-derived PDGFRα-positive cells for functional investigations help us to overcome these challenges.

Results

We demonstrated for the first time that the differentiated PDGFRα-positive cells from iMSCs can be cryopreserved and thawed to be used as per the experimental requirements with prolonged preservation of their characteristics. We assessed the viability of differentiated PDGFRα-positive cells pre- and post-freezing with the subsequent validation of their functional features using flow cytometry, qRT-PCR, and western blotting. We have been successful in demonstrating for the first time that the cryopreservation of previously differentiated PDGFRα-positive cells can be used as a feasible and cost-effective model for experimental reproducibility in functional studies of Diabetes Gastroparesis.

Graphical Abstract
Exploring the alternative virulence determinants PB2 S155N and PA S49Y/D347G that promote mammalian adaptation of the H9N2 avian influenza virus in mice
Authors: Guo Yanna , Bai Xuebing , Liu Zhiyuan , Liang Bing , Zheng Yiqing , Dankar Samar , Ping Jihui
Published: October 2023

The occurrence of human infections caused by avian H9N2 influenza viruses has raised concerns regarding the potential for human epidemics and pandemics. The molecular basis of viral adaptation to a new host needs to be further studied. Here, the bases of nucleotides 627 and 701 of PB2 were changed according to the uncoverable purine-to-pyrimidine transversion to block the development of PB2 627K and 701N mutations during serial passaging in mice. The purpose of this experiment was to identify key adaptive mutations in polymerase and NP genes that were obscured by the widely known host range determinants PB2 627K and 701N. Mouse-adapted H9N2 variants were obtained via twelve serial lung-to-lung passages. Sequence analysis showed that the mouse-adapted viruses acquired several mutations within the seven gene segments (PB2, PB1, PA, NP, HA, NA, and NS). One variant isolate with the highest polymerase activity possessed three substitutions, PB2 S155N, PA S49Y and D347G, which contributed to the highly virulent and mouse-adaptative phenotype. Further studies demonstrated that these three mutations resulted in increased polymerase activity, viral transcription and replication in mammalian cells, severe interstitial pneumonia, excessive inflammatory cellular infiltration and increased growth rates in mice. Our results suggest that the substitution of these three amino acid mutations may be an alternative strategy for H9N2 avian influenza viruses to adapt to mammalian hosts. The continued surveillance of zoonotic H9N2 influenza viruses should also include these mammalian adaptation markers as part of our pandemic preparedness efforts.
YuYin: a multi-task learning model of multi-modal e-commerce background music recommendation
Authors: Ma Le , Wu Xinda , Tang Ruiyuan , Zhong Chongjun , Zhang Kejun
Published: October 2023

Appropriate background music in e-commerce advertisements can help stimulate consumption and build product image. However, many factors like emotion and product category should be taken into account, which makes manually selecting music time-consuming and require professional knowledge and it becomes crucial to automatically recommend music for video. For there is no e-commerce advertisements dataset, we first establish a large-scale e-commerce advertisements dataset Commercial-98K, which covers major e-commerce categories. Then, we proposed a video-music retrieval model YuYin to learn the correlation between video and music. We introduce a weighted fusion module (WFM) to fuse emotion features and audio features from music to get a more fine-grained music representation. Considering the similarity of music in the same product category, YuYin is trained by multi-task learning to explore the correlation between video and music by cross-matching video, music, and tag as well as a category prediction task. We conduct extensive experiments to prove YuYin achieves a remarkable improvement in video-music retrieval on Commercial-98K.
Description of patients with eating disorders by general practitioners: a cohort study and focus on co-management with depression
Authors: Cadwallader Jean Sébastien , Orri Massimiliano , Barry Caroline , Falissard Bruno , Hassler Christine , Huas Caroline
Published: October 2023

Background

International guidelines often state that general practitioners (GPs) provide early management for most patients with eating disorders (EDs). GP management of EDs has not been studied in France. Depressive disorders are often a comorbidity of EDs. The aims of this study were to describe in France the characteristics of people with all subcategories of EDs (Anorexia Nervosa, Bulimia Nervosa, ED Not Otherwise Specified) managed by their GPs and to study the management temporality between depression and all subcategories of EDs.

Methods

Retrospective cohort study of patients with EDs visiting French GPs. Data collected from 1994 through 2009 were extracted from the French society of general electronic health record. A descriptive analysis of the population focused on depression, medication such as antidepressants and anxiolytics, and the management temporality between depression and EDs.

Results

1310 patients aged 8 years or older were seen at least once for an ED by a GP participating in the database out of 355,848 patients, with a prevalence rate of 0.3%. They had a mean age of 35.19 years, 82.67% were women. 41.6% had anorexia nervosa, 26.4% bulimia nervosa, and 32% an ED not otherwise specified. Overall, 32.3% had been managed at least once for depression, and 18.4% had been prescribed an antidepressant of any type at least once. Benzodiazepines had been prescribed at least once for 73.9% of the patients treated for depression. Patients with an ED seen regularly by their GP (“during” profile) received care for depression more frequently than those with other profiles. 60.9% had a single visit with the participating GP for their ED Treatment and management for depression did not precede care for EDs.

Conclusions

Data extracted from the French society of general practice were the only one available in France in primary care about EDs and our study was the only one on this topic. The frequency of visits for EDs was very low in our general practice-based sample. Depressive disorders were a frequent comorbidity of EDs. GPs could manage common early signs of depression and EDs, especially if they improved their communication skills and developed collaborative professional management.
Aggregating multi-time-scale flexibility potentials of battery storages based on open data – a potential analysis
Authors: Lechl Michael , Schoppik Luis , de Meer Hermann
Published: October 2023

Flexibility potentials are mostly provided by centrally coordinated flexibility resources such as natural-gas-fired power plants. However, the decentralization of power generation combined with the decarbonization of the sector due to the energy transition requires the exploration of new types of flexibility resources. In particular, to reduce dependence on natural-gas-fired power plants, it would be desirable to replace them with alternative flexibility resources. Therefore, the objective of this paper is to analyze, using Germany as an example, to what extent the flexibility potential of already existing battery storage systems can replace the flexibility potential of natural-gas-fired power plants. The methodology used is based on a multi-time-scale flexibility model together with an approach for temporal aggregation of flexibility potentials and two approaches for spatial aggregation of flexibility potentials. Based on the methodology and an analysis of publicly available data, a comprehensive potential analysis is carried out. This analysis shows, among others, that existing battery storage systems have a promising potential to replace a considerable number of natural-gas-fired power plants in Germany in terms of their flexibility potentials.
Transformer training strategies for forecasting multiple load time series
Authors: Hertel Matthias , Beichter Maximilian , Heidrich Benedikt , Neumann Oliver , Schäfer Benjamin , Mikut Ralf , Hagenmeyer Veit
Published: October 2023

In the smart grid of the future, accurate load forecasts on the level of individual clients can help to balance supply and demand locally and to prevent grid outages. While the number of monitored clients will increase with the ongoing smart meter rollout, the amount of data per client will always be limited. We evaluate whether a Transformer load forecasting model benefits from a transfer learning strategy, where a global univariate model is trained on the load time series from multiple clients. In experiments with two datasets containing load time series from several hundred clients, we find that the global training strategy is superior to the multivariate and local training strategies used in related work. On average, the global training strategy results in 21.8% and 12.8% lower forecasting errors than the two other strategies, measured across forecasting horizons from one day to one month into the future. A comparison to linear models, multi-layer perceptrons and LSTMs shows that Transformers are effective for load forecasting when they are trained with the global training strategy.
Quality properties of IEC 62559 use cases and SGAM models
Authors: Kuchenbuch René , Schütz Johann , Sauer Jürgen
Published: October 2023

The Smart Grid Architecture Model (SGAM) and the IEC 62559-2 Use Case Template are essential representatives of the Requirements Engineering of energy systems to promote interoperability. In particular, the quality of the use case descriptions and SGAM models is crucial for the system understanding. In order to measure and assess the quality of given use cases of the SGAM models, the aim of this research is to determine those requirements and quality characteristics. Based on a literature review, general best practices for deriving use cases and SGAM models are obtained. The results can be used to concept far-reaching supportive systems or to evaluate IEC 62559 use case descriptions and SGAM models.
Generating synthetic load profiles of residential heat pumps: a k-means clustering approach
Authors: Semmelmann Leo , Jaquart Patrick , Weinhardt Christof
Published: October 2023

The creation of synthetic heat pump load profiles is essential for energy system modeling and simulations. This paper proposes a methodology to create synthetic heat pump load profiles based on the k-means algorithm and a data set from water-to-water heat pumps from Hamelin, Germany. The quality of the generated load profiles is shown according to load factors, load distribution curves and the Pearson correlation coefficient, and is also applied on two exemplary geographies in Germany. We publish our work open-source and provide a web-based heat pump load profile generator.
Quantifying the resilience of ICT-enabled grid services in cyber-physical energy system
Authors: Narayan Anand , Brand Michael , Lehnhoff Sebastian
Published: October 2023

Information and Communication Technology (ICT) is vital for the operation of modern power systems, giving rise to Cyber-Physical Energy Systems (CPESs). ICT enables the grid services (GSs) needed for monitoring and controlling the physical parameters of the power system, especially for remedying the impact of disturbances. But the ICT integration makes the overall system more complex, leading to new and unforeseen disturbances. This motivates the need for a resilient system design capable of absorbing and recovering from such disturbances. The current state of the art lacks a comprehensive resilience assessment of ICT-enabled GSs in CPESs. To address this, a novel method and metrics to assess the resilience of GSs in CPESs are presented in this paper. An operational state model of a GS, with three states, i.e., normal, limited and failed, is used to capture its performance, which is essential for quantifying its resilience. Sequential Monte Carlo simulations are performed with the model to capture the behaviour of ICT components to compute the operational state trajectory of the GSs. Metrics are then derived to quantify the resilience and its constituting phases. The method is demonstrated using two ICT system designs for the CIGRE MV benchmark grid, considering the state estimation as an exemplary GS. The simulation results show that the proposed method can capture the differences between ICT system designs with regard to resilience metrics. The contribution can, therefore, be used to analyse, compare and potentially improve the resilience of ICT system designs for CPES.
A model-based approach for converting CGMES power system models into operational networks
Authors: Memari Ammar , Aljamous Adam
Published: October 2023

This paper presents a model-based approach for converting Common Grid Model Exchange Specification (CGMES) power system models into operational networks. The approach utilizes the Model-Driven Architecture (MDA), borrowed from software engineering, to create a Platform-Independent Model (PIM) saved in a graph database, neo4j. The PIM is then transformed into Platform-Specific Model (PSM) using cypher queries. Finally, from the PSM, a Pandapower network is generated that is used later for running power flow simulations. The Common Information Model (CIM) ontology classes, which form the metamodel behind CGMES, are loaded into the same database, connecting them to the grid instances, allowing for automatic validation and inference. The proposed approach has been successfully applied to large-scale real-world grids that include renewable energy sources.
A methodological framework for geospatial modelling of hydrogen demand in cities
Authors: Beck Stefan , Fischer David
Published: October 2023

Urban energy system planning is vital for cities shifting towards a more sustainable and integrated energy system. Hydrogen is considered one of the most promising solutions in future energy systems. Previous work on hydrogen energy systems predominantly analysed hydrogen models on a national level or only parts of the mobility sector. This indicates a research gap for geospatial models that include multiple sectors in which hydrogen can be used. These models can be used to support decision-making processes around the hydrogen economy in cities. This study presents a holistic model addressing the geospatial modelling of hydrogen demand in urban areas. It proposes a method that integrates a variety of open source data, including geodata, earth observation data and energy data to estimate hydrogen demand top down for the industrial feedstock (steel, ammonia, organic chemistry), process heating, and mobility (buses, trucks, trains, airplanes, ships) sectors. The proposed method can also be extended to different sectors. The method is validated by modelling the hydrogen demand in all German cities and benchmarking it with national studies. This study’s results are within the same range as the results of national studies. For this paper, the method is applied for two case studies in Freiburg im Breisgau and Frankfurt am Main. Applying this method in urban areas shows potential hydrogen demand hotspots in these areas. The model’s results help policymakers and industry stakeholders make informed decisions about the development of hydrogen infrastructure and facilitate the adoption of hydrogen as a low-carbon energy carrier. Future research could explore the temporal aspects of hydrogen demand and the spatial influence of hydrogen demand on future hydrogen production facilities such as electrolysers.
Cost Evaluation Analysis of Genetic Testing and Tailored Adjuvant Imatinib in Patients With Resected High-Risk GI Stromal Tumors: The Brazilian Perspective
Authors: Lopes David Bruna Bianca , Nazareth Aguiar Junior Pedro , Costa e Silva Matheus , Dienstmann Rodrigo , Gil Ferreira Carlos , Serrano César
Published: October 2023

PURPOSE

Mutations of the KIT gene are the molecular hallmark of most GI stromal tumors (GISTs). Imatinib has revolutionized GIST treatment. Adjuvant imatinib for 3 years is the standard of care for high-risk resected GIST. However, the GIST molecular biologic profile has found different responses to this approach. Despite this, genetic testing at diagnosis is not a routine and empirical adjuvant imatinib remains the rule. Barriers to genetic profiling include concerns about the cost and utility of testing. This analysis aims to determine whether targeted genetic testing reduces costs as an ancillary tool for a limited-resource scenario instead of adjuvant empirical imatinib in patients with resected high-risk GIST.

METHODS

The cost evaluation analysis of molecular testing for GIST was based on the Cost of Preventing an Event (COPE), considering the Number Needed to Treat and the costs of each test compared with the cost of 3-year empirical adjuvant imatinib and real treatment costs (median number of cycles) from the public and private Brazilian Healthcare System's perspective. The analysis compared the costs of the molecular tests (broad next-generation sequencing [NGS], GS Infinity DNA/RNA assay, and targeted NGS: GS Focus GIST and the Fleury GIST Tumor DNA sequencing panel), costs of drug acquisition, considering discounts (imatinib mesylate and Glivec), and the costs of supportive care.

RESULTS

In both scenarios, public and private, regardless of the use of imatinib or Glivec, tailoring adjuvant treatment reduced costs, irrespective of the number of cycles. The only exception was the combination of the broad NGS test and imatinib in the Public Healthcare System.

CONCLUSION

The molecularly tailored adjuvant imatinib reduced costs considering the COPE of available NGS tests for both the public and private Brazilian health care systems.
Adoption of Universal Testing in Endometrial Cancers for Microsatellite Instability Using Next-Generation Sequencing
Authors: Rodriguez Isabel V. , Strickland Sarah , Wells David , Manhardt Enna , Konnick Eric Q. , Garcia Rochelle , Swisher Elizabeth , Kilgore Mark , Norquist Barbara
Published: October 2023

Lynch syndrome screening by NGS in endometrial cancer allows concurrent molecular classification with one test.
Imperial Gift
Authors: Tran Ann Ngoc
Published: October 2023

This article theorizes and historicizes soap, a medical “gift” distributed by the US military to villages and hamlets in South Vietnam, as a commodity and as an infrastructure in the American war in Vietnam. During the war, soap not only operated as a tool to clean those the US military deemed dirty and uncivilized but also brokered the ideological movement of empire from the nation-state to the occupied regions of the Vietnamese South. Wartime US humanitarianism proffered soap as a counterinsurgent weapon of soft power and as an infrastructural poetic that securitized US empire against the rising tide of communist insurgency. Reading against the hegemonic archival practices that venerate the gifting of soap as benevolent militarism, the article moves to examine the anarchic practice of South Vietnamese black marketeering, which redeployed soap as an illegal market commodity to intercept the movement of US empire and, as a practice that arose in response to wartime capitalism and militarization, allowed natives to command new social relations at the cost of disrupting the Republic of Vietnam’s flows of capital and the United States’ ongoing war campaign. As an informal, opaque, and yet thriving infrastructure of its own, the black market fostered insurgent survival strategies that repurposed military supplies and gifts as versatile commodities, allowing even soap to escape its original containment as a civilizing agent and giving it new uses and meanings.
The Human Tide
Authors: Gao Yuan
Published: October 2023

This article investigates how cultural workers from the 1950s to the 1970s served China’s hydraulic engineering campaign in artworks depicting human resource extraction. Focusing on Tian Han’s drama The Caprice of the Ming Tombs Reservoir (1958) and Jiang Yunchuan’s documentary Red Flag Canal (1970), the article tells two tales of Chinese hydraulic construction to analyze the theatrical and cinematic aesthetics of socialist labor reform and rural industrialization. In China’s history of transforming water from a natural threat to a natural resource, Tian Han and Jiang Yunchuan represent the Chinese cultural workers who used their works to mobilize the masses to navigate the hostile natural environment and overcome technological insufficiency, portraying the body as corporeal machine. This mode of cultural representation went beyond revolutionary culture’s conventional task of reinforcing class consciousness. Instead, it aimed to generate and maintain the energy of the infrastructure builders to change the nature of labor in socialist industrial planning. The Chinese cultural works on hydraulic engineering draw attention to the materiality of the laboring bodies often ignored in current infrastructure scholarship.