bluuebunny/crossref_metadata_2025 huggingface dataset:
Sensopeptidomic Kinetic Approach Combined with Decision Trees and Random Forests to Study the Bitterness during Enzymatic Hydrolysis Kinetics of Micellar Caseins
Authors: Daher Dahlia , Deracinois Barbara , Courcoux Philippe , Baniel Alain , Chollet Sylvie , Froidevaux Rénato , Flahaut Christophe
Published: June 2021

Protein hydrolysates are, in general, mixtures of amino acids and small peptides able to supply the body with the constituent elements of proteins in a directly assimilable form. They are therefore characterised as products with high nutritional value. However, hydrolysed proteins display an unpleasant bitter taste and possible off-flavours which limit the field of their nutrition applications. The successful identification and characterisation of bitter protein hydrolysates and, more precisely, the peptides responsible for this unpleasant taste are essential for nutritional research. Due to the large number of peptides generated during hydrolysis, there is an urgent need to develop methods in order to rapidly characterise the bitterness of protein hydrolysates. In this article, two enzymatic hydrolysis kinetics of micellar milk caseins were performed for 9 h. For both kinetics, the optimal time to obtain a hydrolysate with appreciable organoleptic qualities is 5 h. Then, the influence of the presence or absence of peptides and their intensity over time compared to the different sensory characteristics of hydrolysates was studied using heat maps, random forests and regression trees. A total of 22 peptides formed during the enzymatic proteolysis of micellar caseins and influencing the bitterness the most were identified. These methods represent simple and efficient tools to identify the peptides susceptibly responsible for bitterness intensity and predict the main sensory feature of micellar casein enzymatic hydrolysates.
Age Prediction of Human Based on DNA Methylation by Blood Tissues
Authors: Zhang Jiansheng , Fu Hongli , Xu Yan
Published: June 2021

In recent years, scientists have found a close correlation between DNA methylation and aging in epigenetics. With the in-depth research in the field of DNA methylation, researchers have established a quantitative statistical relationship to predict the individual ages. This work used human blood tissue samples to study the association between age and DNA methylation. We built two predictors based on healthy and disease data, respectively. For the health data, we retrieved a total of 1191 samples from four previous reports. By calculating the Pearson correlation coefficient between age and DNA methylation values, 111 age-related CpG sites were selected. Gradient boosting regression was utilized to build the predictive model and obtained the R2 value of 0.86 and MAD of 3.90 years on testing dataset, which were better than other four regression methods as well as Horvath’s results. For the disease data, 354 rheumatoid arthritis samples were retrieved from a previous study. Then, 45 CpG sites were selected to build the predictor and the corresponded MAD and R2 were 3.11 years and 0.89 on the testing dataset respectively, which showed the robustness of our predictor. Our results were better than the ones from other four regression methods. Finally, we also analyzed the twenty-four common CpG sites in both healthy and disease datasets which illustrated the functional relevance of the selected CpG sites.
TOMM40 RNA Transcription in Alzheimer’s Disease Brain and Its Implication in Mitochondrial Dysfunction
Authors: Lee Eun-Gyung , Chen Sunny , Leong Lesley , Tulloch Jessica , Yu Chang-En
Published: June 2021

Increasing evidence suggests that the Translocase of Outer Mitochondria Membrane 40 (TOMM40) gene may contribute to the risk of Alzheimer’s disease (AD). Currently, there is no consensus as to whether TOMM40 expression is up- or down-regulated in AD brains, hindering a clear interpretation of TOMM40’s role in this disease. The aim of this study was to determine if TOMM40 RNA levels differ between AD and control brains. We applied RT-qPCR to study TOMM40 transcription in human postmortem brain (PMB) and assessed associations of these RNA levels with genetic variants in APOE and TOMM40. We also compared TOMM40 RNA levels with mitochondrial functions in human cell lines. Initially, we found that the human genome carries multiple TOMM40 pseudogenes capable of producing highly homologous RNAs that can obscure precise TOMM40 RNA measurements. To circumvent this obstacle, we developed a novel RNA expression assay targeting the primary transcript of TOMM40. Using this assay, we showed that TOMM40 RNA was upregulated in AD PMB. Additionally, elevated TOMM40 RNA levels were associated with decreases in mitochondrial DNA copy number and mitochondrial membrane potential in oxidative stress-challenged cells. Overall, differential transcription of TOMM40 RNA in the brain is associated with AD and could be an indicator of mitochondrial dysfunction.
In Silico Prediction of Transcription Factor Collaborations Underlying Phenotypic Sexual Dimorphism in Zebrafish (Danio rerio)
Authors: Hosseini Shahrbanou , Schmitt Armin Otto , Tetens Jens , Brenig Bertram , Simianer Henner , Sharifi Ahmad Reza , Gültas Mehmet
Published: June 2021

The transcriptional regulation of gene expression in higher organisms is essential for different cellular and biological processes. These processes are controlled by transcription factors and their combinatorial interplay, which are crucial for complex genetic programs and transcriptional machinery. The regulation of sex-biased gene expression plays a major role in phenotypic sexual dimorphism in many species, causing dimorphic gene expression patterns between two different sexes. The role of transcription factor (TF) in gene regulatory mechanisms so far has not been studied for sex determination and sex-associated colour patterning in zebrafish with respect to phenotypic sexual dimorphism. To address this open biological issue, we applied bioinformatics approaches for identifying the predicted TF pairs based on their binding sites for sex and colour genes in zebrafish. In this study, we identified 25 (e.g., STAT6-GATA4; JUN-GATA4; SOX9-JUN) and 14 (e.g., IRF-STAT6; SOX9-JUN; STAT6-GATA4) potentially cooperating TFs based on their binding patterns in promoter regions for sex determination and colour pattern genes in zebrafish, respectively. The comparison between identified TFs for sex and colour genes revealed several predicted TF pairs (e.g., STAT6-GATA4; JUN-SOX9) are common for both phenotypes, which may play a pivotal role in phenotypic sexual dimorphism in zebrafish.
The Diagnostic Journey of a Patient with Prader–Willi-Like Syndrome and a Unique Homozygous SNURF-SNRPN Variant; Bio-Molecular Analysis and Review of the Literature
Authors: Pellikaan Karlijn , van Woerden Geeske M. , Kleinendorst Lotte , Rosenberg Anna G. W. , Horsthemke Bernhard , Grosser Christian , van Zutven Laura J. C. M. , van Rossum Elisabeth F. C. , van der Lely Aart J. , Resnick James L. , Brüggenwirth Hennie T. , van Haelst Mieke M. , de Graaff Laura C. G.
Published: June 2021

Prader–Willi syndrome (PWS) is a rare genetic condition characterized by hypotonia, intellectual disability, and hypothalamic dysfunction, causing pituitary hormone deficiencies and hyperphagia, ultimately leading to obesity. PWS is most often caused by the loss of expression of a cluster of genes on chromosome 15q11.2-13. Patients with Prader–Willi-like syndrome (PWLS) display features of the PWS phenotype without a classical PWS genetic defect. We describe a 46-year-old patient with PWLS, including hypotonia, intellectual disability, hyperphagia, and pituitary hormone deficiencies. Routine genetic tests for PWS were normal, but a homozygous missense variant NM_003097.3(SNRPN):c.193C>T, p.(Arg65Trp) was identified. Single nucleotide polymorphism array showed several large regions of homozygosity, caused by high-grade consanguinity between the parents. Our functional analysis, the ‘Pipeline for Rapid in silico, in vivo, in vitro Screening of Mutations’ (PRiSM) screen, showed that overexpression of SNRPN-p.Arg65Trp had a dominant negative effect, strongly suggesting pathogenicity. However, it could not be confirmed that the variant was responsible for the phenotype of the patient. In conclusion, we present a unique homozygous missense variant in SNURF-SNRPN in a patient with PWLS. We describe the diagnostic trajectory of this patient and the possible contributors to her phenotype in light of the current literature on the genotype–phenotype relationship in PWS.
A Case of Nosocomial Outbreak of Pantoea agglomerans Related to Parenteral Nutrition Procedures
Authors: Mirtella Dora , Fedeli Piergiorgio , Scendoni Roberto , Cannovo Nunzia , Cingolani Mariano
Published: June 2021

Pantoea agglomerans is a Gram-negative bacterium that infrequently infects humans. Most reports involving it are about infections in soft tissues or bone/joint infections caused by contamination from soil or penetrating trauma by vegetation, such as thorns and splinters. More frequently, it is found as an opportunistic pathogen in immunocompromised patients. It is not rare to find reports of bacteremia and sepsis from contamination of intravenous fluid, parenteral nutrition, blood products and anesthetic agents—that is, through contamination of medical devices and products. We reported a bacterial infection epidemic occurred in 2011 in a hospital in middle Italy which involved different hospital wards; Pantoea agglomerans was isolated from blood cultures of all infected patients and the source of infection was identified in contaminated parenteral nutrition prepared from the local pharmacy.
The Effects of Social Support and Morbidities on Self-Rated Health among Migrant Elderly Following Children to Jinan, China
Authors: Wei Suqing , Kong Fanlei , Li Shixue
Published: June 2021

Social support has been demonstrated to be associated with the health status of old adults, but no study has clarified the relationship between social support, morbidities and self-rated health among the migrant elderly following children (MEFC) to new cities. This study aimed to explore the effect of social support and morbidities on self-rated health among MEFC to Jinan, China. A total of 656 MEFC were included in this study by using multi-stage cluster random sampling. Social support was measured by the Social Support Rating Scale. Correlation analysis and multivariable logistic regression analysis were employed to clarify the association between social support, morbidities and self-rated health among the MEFC. Approximately 75.9% of the MEFC rated their health as good. Logistic regression analysis showed that MEFC who lived with family were more likely to have a higher level of self-rated health. In addition to social support, body mass index (BMI), monthly income, one-year living style, the presence of an elevator, heart disease, stroke, duration of chronic disease, and outpatient service attendance were also associated with the self-rated health of MEFC. Social support and morbidities were significantly associated with self-rated health among MEFC. Targeted policies should be made to improve social support status and lower the morbidities in MEFC.
Postharvest Fumigation of Fresh Citrus with Cylinderized Phosphine to Control Bean Thrips (Thysanoptera: Thripidae)
Authors: Walse Spencer S. , Jimenez Leonel R.
Published: June 2021

Bean thrips (BT), Caliothrips fasciatus (Pergande), is a pest of concern to certain countries that import fresh citrus fruit from California, USA. A series of laboratory-scale exploratory fumigations with phosphine at 4.9 ± 0.3 °C (mean ± 2 SD; x¯±2s) were conducted to evaluate the postharvest control of adult BT. Models of the duration–mortality response predicted ca. 99% mortality of BT populations when headspace concentrations of phosphine, [PH3], are maintained at levels ≥0.4 g m−3 (250 ppmv (µL L−1)) and ≤1.5 g m−3 (1000 ppmv (µL L−1)) for 12 h, with the duration representing the lower bound of the 95% confidence level (CL). Confirmatory fumigations, each lasting 12 h, were then conducted using BT-infested sweet oranges, Citrus sinensis (L.), at pulp temperature (T) ≤ 5 °C to corroborate the exploratory results. Three formulations of cylinderized phosphine were used: 1.6% phosphine by volume in nitrogen, VAPORPH3OS®, and ECOFUME®, all applied at two levels, ca. 1.5 g m−3 (1000 ppmv (µL L−1)), as well as 0.5 g m−3 (300 ppmv (µL L−1)). Collectively, across the formulations, an applied dose of ca. 1.5 g m−3 (1000 ppmv (µL L−1)) resulted in 0 survivors from 38,993 (probit 8.60, 95% CL; probit 9, 72% CL) treated BT, while an applied dose of 0.5 g m−3 (300 ppmv (µL L−1)) resulted in 0 survivors from 31,204 (probit 8.56, 95% CL; probit 9, 70% CL) treated BT. Results were discussed in the context of commercial and operational features of quarantine and pre-shipment (QPS) uses of phosphine to treat fresh fruit and, specifically, the control of BT in fresh citrus exported from California, USA, to Australia.
Yoga and Mindfulness Interventions for Preschool-Aged Children in Educational Settings: A Systematic Review
Authors: Sun Yaoyao , Lamoreau Renee , O’Connell Samantha , Horlick Raquel , Bazzano Alessandra N.
Published: June 2021

Early childhood and the pre-school stage of development constitute a dynamic period for acquisition of social-emotional competencies. Yoga and mindfulness practices (YMP) have become increasingly used in schools for social emotional learning, but less is known about their utility in early childhood settings. A systematic review using PRISMA guidelines was undertaken to explore the effect of YMP on social emotional function among preschool-aged children (3–5 years). The review resulted in identification of 1115 records, of which 80 full text articles were screened, with final inclusion of 16 studies. Included studies evaluated the effect of YMP on social-emotional functioning, and identified the potential for YMP to improve regulatory skills such as behavioral self-regulation and executive function. Among studies reviewed, 13 reported improvements in these domains, but quality appraisal indicated significant variability in risk of bias across studies, and heterogeneity of outcome measurements hindered comparison. Programs appeared to produce better results when implemented for at least 6 weeks and among children who had lower baseline social-emotional functioning. YMP constitute a promising strategy for social emotional development in early childhood settings, but additional rigorously designed studies are needed to expand understanding of how and why these programs are effective.
An Innovative Approach to Determining the Contribution of Saharan Dust to Pollution
Authors: Lotrecchiano Nicoletta , Capozzi Vincenzo , Sofia Daniele
Published: June 2021

Air quality is one of the hot topics of today, and many people are interested in it due to the harmful effects that environmental pollution has on human health. For this reason, in recent years, measurement systems based on advanced technology have been implemented to integrate national air quality networks. This study aimed to analyze the air quality data of the monitoring network of the regional agency for environmental protection of the Campania region (Italy), integrated with a monitoring station based on IoT technology to highlight criticalities in the levels of pollution. The data used was from the month of February 2021 and measured in a medium-large city in southern Italy. In-depth analyses showed that two events related to Saharan dust occurred, which led to an increase in the measured PM10 values.
Proposal for the Inclusion of Tobacco Use in Suicide Risk Scales: Results of a Meta-Analysis
Authors: Echeverria Iván , Cotaina Miriam , Jovani Antonio , Mora Rafael , Haro Gonzalo , Benito Ana
Published: June 2021

There is an association between smoking and suicide, even though the direction and nature of this relationship remains controversial. This meta-analysis aimed to evaluate the association between smoking and suicidal behaviours (ideation, planning, suicide attempts, and death by suicide). On 24 August 2020, we searched the PubMed, Cochrane library, Scopus, Web of Science, TRIP, and SCIENCE DIRECT databases for relevant articles on this topic. Twenty prospective cohort studies involving 2,457,864 participants were included in this meta-analysis. Compared with never smokers, former and current smokers had an increased risk of death by suicide (relative risk [RR] = 1.31; 95% CI [1.13, 1.52] and RR = 2.41; 95% CI [2.08, 2.80], respectively), ideation (RR = 1.35; 95% CI [1.31, 1.39] and RR = 1.84; 95% CI [1.21, 2.78]), and attempted suicide (RR = 1.27; 95% CI [0.56, 2.87] and RR = 1.71; 95% CI [0.73, 3.97]). Moreover, compared to never smokers, current smoker women (RR = 2.51; 95% CI [2.06–3.04] had an increased risk of taking their own life (Q = 13,591.53; p < 0.001) than current smoker men (RR = 2.06; 95% CI [1.62–2.62]. Furthermore, smoking exposure (former and current smokers) was associated with a 1.74-fold increased risk (95% CI [1.54, 1.96]) of suicidal behaviour (death by suicide, ideation, planning, or attempts). Thus, because of the prospective relationship between smoking and suicidal behaviours, smoking should be included in suicide risk scales as a useful and easy item to evaluate suicide risk.
Why We Will Continue to Lose Our Battle with Cancers If We Do Not Stop Their Triggers from Environmental Pollution
Authors: Cazzolla Gatti Roberto
Published: June 2021

Besides our current health concerns due to COVID-19, cancer is a longer-lasting and even more dramatic pandemic that affects almost a third of the human population worldwide. Most of the emphasis on its causes has been posed on genetic predisposition, chance, and wrong lifestyles (mainly, obesity and smoking). Moreover, our medical weapons against cancers have not improved too much during the last century, although research is in progress. Once diagnosed with a malignant tumour, we still rely on surgery, radiotherapy, and chemotherapy. The main problem is that we have focused on fighting a difficult battle instead of preventing it by controlling its triggers. Quite the opposite, our knowledge of the links between environmental pollution and cancer has surged from the 1980s. Carcinogens in water, air, and soil have continued to accumulate disproportionally and grow in number and dose, bringing us to today’s carnage. Here, a synthesis and critical review of the state of the knowledge of the links between cancer and environmental pollution in the three environmental compartments is provided, research gaps are briefly discussed, and some future directions are indicated. New evidence suggests that it is relevant to take into account not only the dose but also the time when we are exposed to carcinogens. The review ends by stressing that more dedication should be put into studying the environmental causes of cancers to prevent and avoid curing them, that the precautionary approach towards environmental pollutants must be much more reactionary, and that there is an urgent need to leave behind the outdated petrochemical-based industry and goods production.
The Influence of Self-Efficacy and Locus of Control on Body Image: A Path-Analysis in Aspiring Fashion Models, Athletes and Students
Authors: Di Corrado Donatella , Coco Marinella , Guarnera Maria , Maldonato Nelson Mauro , Quartiroli Alessandro , Magnano Paola
Published: June 2021

It is widely documented that negative body image is a significant public health concern due to its association with symptoms of disordered eating and worse psychological well-being. The purpose of the study was to develop a path model of intrapersonal dimensions (self-efficacy and internal locus of control) as antecedents of perceived stress toward females’ body dissatisfaction and eating attitude disorders. A total of 300 females, including 100 aspiring fashion models, 100 athletes and 100 students (controls), between 15 and 24 years of age (M = 19.6, SD = 1.85) participated in the study. Measures included level of psychological stress, self-efficacy and locus of control dimensions, body dissatisfaction and eating attitude disorder indices. A path analysis confirmed our research hypothesis. Comparing the three subsamples, we found better fit indexes in the two subgroups with elevated investment on their body image with respect the control group. More specifically, the model in the group of aspiring fashion models showed the best fit index. These results indicated that aspiring fashion models have a strong desire to maintain their low body mass or to become thinner. For this reason, a suitable involvement of expert health workers in the nutritional and psychological field could be extremely essential in the fashion world to maintain a healthier well-being.
Lack of Informations about COVID-19 Vaccine: From Implications to Intervention for Supporting Public Health Communications in COVID-19 Pandemic
Authors: Guljaš Silva , Bosnić Zvonimir , Salha Tamer , Berecki Monika , Krivdić Dupan Zdravka , Rudan Stjepan , Majnarić Trtica Ljiljana
Published: June 2021

Lack of knowledge and mistrust towards vaccines represent a challenge in achieving the vaccination coverage required for population immunity. The aim of this study is to examine the opinion that specific demographic groups have about COVID-19 vaccination, in order to detect potential fears and reasons for negative attitudes towards vaccination, and to gain knowledge on how to prepare strategies to eliminate possible misinformation that could affect vaccine hesitancy. The data collection approach was based on online questionnaire surveys, divided into three groups of questions that followed the main postulates of the health belief theory—a theory that helps understanding a behaviour of the public in some concrete surrounding in receiving preventive measures. Ordinary least squares regression analyses were used to examine the influence of individual factors on refusing the vaccine, and to provide information on the perception of participants on the danger of COVID-19 infection, and on potential barriers that could retard the vaccine utility. There was an equal proportion of participants (total number 276) who planned on receiving the COVID-19 vaccine (37%), and of those who did not (36.3%). The rest (26.7%) of participants were still indecisive. Our results indicated that attitudes on whether to receive the vaccine, on how serious consequences might be if getting the infection, as well as a suspicious towards the vaccine efficacy and the fear of the vaccine potential side effects, may depend on participants’ age (<40 vs. >40 years) and on whether they are healthcare workers or not. The barriers that make participants‘ unsure about of receiving the vaccine, such as a distrust in the vaccine efficacy and safety, may vary in different socio-demographic groups and depending on which is the point of time in the course of the pandemic development, as well as on the vaccine availability and experience in using certain vaccine formulas. There is a pressing need for health services to continuously provide information to the general population, and to address the root causes of mistrust through improved communication, using a wide range of policies, interventions and technologies.
High Temporal Resolution Land Use Regression Models with POI Characteristics of the PM2.5 Distribution in Beijing, China
Authors: Zhang Yan , Cheng Hongguang , Huang Di , Fu Chunbao
Published: June 2021

PM2.5 is one of the primary components of air pollutants, and it has wide impacts on human health. Land use regression models have the typical disadvantage of low temporal resolution. In this study, various point of interests (POIs) variables are added to the usual predictive variables of the general land use regression (LUR) model to improve the temporal resolution. Hourly PM2.5 concentration data from 35 monitoring stations in Beijing, China, were used. Twelve LUR models were developed for working days and non-working days of the heating season and non-heating season, respectively. The results showed that these models achieved good fitness in winter and summer, and the highest R2 of the winter and summer models were 0.951 and 0.628, respectively. Meteorological factors, POIs, and roads factors were the most critical predictive variables in the models. This study also showed that POIs had time characteristics, and different types of POIs showed different explanations ranging from 5.5% to 41.2% of the models on working days or non-working days, respectively. Therefore, this study confirmed that POIs can greatly improve the temporal resolution of LUR models, which is significant for high precision exposure studies.
Prenatal Bisphenol a Exposure, DNA Methylation, and Low Birth Weight: A Pilot Study in Taiwan
Authors: Huang Yu-Fang , Chang Chia-Huang , Chen Pei-Jung , Lin I-Hsuan , Tsai Yen-An , Chen Chian-Feng , Wang Yu-Chao , Huang Wei-Yun , Tsai Ming-Song , Chen Mei-Lien
Published: June 2021

Prenatal exposure to bisphenol A (BPA) may increase the risk of abnormal birth outcomes, and DNA methylation might mediate these adverse effects. This study aimed to investigate the effects of maternal BPA exposure on maternal and fetal DNA methylation levels and explore whether epigenetic changes are related to the associations between BPA and low birth weight. We collected urine and blood samples originating from 162 mother-infant pairs in a Taiwanese cohort study. We measured DNA methylation using the Illumina Infinium HumanMethylation 450 BeadChip in 34 maternal blood samples with high and low BPA levels based on the 75th percentile level (9.5 μg/g creatinine). Eighty-seven CpGs with the most differentially methylated probes possibly interacting with BPA exposure or birth weight were selected using two multiple regression models. Ingenuity pathway analysis (IPA) was utilized to narrow down 18 candidate CpGs related to disease categories, including developmental disorders, skeletal and muscular disorders, skeletal and muscular system development, metabolic diseases, and lipid metabolism. We then validated these genes by pyrosequencing, and 8 CpGs met the primer design score requirements in 82 cord blood samples. The associations among low birth weight, BPA exposure, and DNA methylation were analyzed. Exposure to BPA was associated with low birth weight. Analysis of the epigenome-wide findings did not show significant associations between BPA and DNA methylation in cord blood of the 8 CpGs. However, the adjusted odds ratio for the dehydrogenase/reductase member 9 (DHRS9) gene, at the 2nd CG site, in the hypermethylated group was significantly associated with low birth weight. These results support a role of BPA, and possibly DHRS9 methylation, in fetal growth. However, additional studies with larger sample sizes are warranted.
Bibliometric Evaluation of Global Tai Chi Research from 1980–2020
Authors: You Yanwei , Min Leizi , Tang Meihua , Chen Yuquan , Ma Xindong
Published: June 2021

While studies on the health benefits of Tai Chi have sprung up over the past four decades, few have engaged in collecting global data, estimating the developing trends, and conducting reviews from the perspective of visualization and bibliometric analysis. This study aimed to provide a summary of the global scientific outputs on Tai Chi research from 1980 to 2020, explore the frontiers, identify cooperation networks, track research trends and highlight emerging hotspots. Relevant publications were downloaded from the Web of Science Core Collection (WoSCC) database between 1980 and 2020. Bibliometric visualization and comparative analysis of authors, cited authors, journals, co-cited journals, institutions, countries, references, and keywords were systematically conducted using CiteSpace software. A total of 1078 publications satisfied the search criteria, and the trend of annual related publications was generally in an upward trend, although with some fluctuations. China (503) and Harvard University (74) were the most prolific country and institution, respectively. Most of the related researches were published in the journals with a focus on sport sciences, alternative medicine, geriatrics gerontology, and rehabilitation. Our results indicated that the current concerns and difficulties of Tai Chi research are “Intervention method”, “Targeted therapy”, “Applicable population”, “Risk factors”, and “Research quality”. The frontiers and promising domains of Tai Chi exercise in the health science field are preventions and rehabilitations of “Fall risk”, “Cardiorespiratory related disease”, “Stroke”, “Parkinson’s disease”, and “Depression”, which should receive more attention in the future.
Factors Related to Depression Associated with Chewing Problems in the Korean Elderly Population
Authors: Chun Hyejin , Doo Miae
Published: June 2021

Unlike younger adults, depression in older adults is sometimes related to chewing problems. This study examined the risk factors related to depression associated with chewing problems in 3747 elderly individuals using the Korean National Health and Nutrition Examination Survey. Approximately 41.2% of the total subjects reported chewing problems. There were significant differences in age, education, marital status, individual income, current smoking status, and aerobic physical activity in relation to chewing problems (p < 0.001 for all). The subjects who experienced chewing problems showed a higher score on the EuroQoL 5 Dimension index (p < 0.001) but a lower health-related quality of life than those with no chewing problems (p < 0.001). The prevalence of depression, which was classified by the patient health questionnaire—9, in subjects with chewing problems was approximately 2 times higher than that in those with no chewing problems (p < 0.001). Subjects with chewing problems were found to have a 1.945-fold higher adjusted risk of depression than those who did not have chewing problems (95% CI = 1.583–2.390, p < 0.001), and subjects with high protein consumption showed a 1.410-fold greater risk of depression (95% CI = 1.144–1.739, p = 0.001) than those with low protein consumption.
Quality Evaluation of Health Services Using the Kano Model in Two Hospitals in Peru
Authors: Barrios-Ipenza Fernando , Calvo-Mora Arturo , Criado-García Fernando , Curioso Walter H.
Published: June 2021

Public–private partnerships (PPP) represent an alternative model of health management focused on improving the quality of health services, particularly in emerging countries. To date, a systematic method to improve the perceived quality of health services by healthcare users in Peru has not been established. The purpose of this study was to evaluate the quality of health services in two PPP hospitals in Peru using the Kano model. A prospective cross-sectional descriptive observational study was carried out through a health service satisfaction survey using the Kano model methodology, measuring six categories of attributes. A total of 250 users of the health services were surveyed in the two PPP hospitals, located in Lima and Callao, using non-probability convenience sampling. Of the 31 attributes evaluated by the patients, 27 (81%) were classified as having a one-dimensional-type attribute, 3 (10%) were reported as mandatory, and 1 (3%) was considered as inverse. These results suggest that the presence of most of the attributes evaluated was relevant to maintaining the level of user satisfaction and that the absence of these attributes generated dissatisfaction in the users. The results showed that the users’ evaluation of health services was multidimensional—namely, their evaluation was focused not only on the interaction space between the patient and medical personnel but also addressed other interaction services.
Associations of Sociodemographic Factors and Health Behaviors with the Emotional Well-Being of Adolescents during the COVID-19 Pandemic in Brazil
Authors: Szwarcwald Célia Landmann , Malta Deborah Carvalho , Barros Marilisa Berti de Azevedo , de Souza Júnior Paulo Roberto Borges , Romero Dália , de Almeida Wanessa da Silva , Damacena Giseli Nogueira , Werneck André Oliveira , da Silva Danilo Rodrigues Pereira , Lima Margareth Guimarães , Gomes Crizian Saar , Azevedo Luiz Otávio , Ferreira Arthur Pate de Souza , Gracie Renata , de Pina Maria de Fátima
Published: June 2021

This cross-sectional study utilizes data from a nationwide web-based survey aimed to identify the factors affecting the emotional well-being of Brazilian adolescents aged 12–17 during the period of school closures and confinement. Data collection took place from 27 June to 17 September 2020. We used the “virtual snowball” sampling method, and students from private and public schools were included. A total of 9470 adolescents were analyzed. A hierarchical logistic regression model was used to find the factors associated with reporting at least two of three self-reported problems—sadness, irritability, and sleep problems. The main proximal factor was loneliness (AdjOR = 8.12 p < 0.001). Problems related to school closures also played an important role. Regular intake of fruits and vegetables, as well as physical activity, demonstrated a positive influence on emotional well-being, while excessive screen time (AdjOR = 2.05, p < 0.001) and alcohol consumption negatively affected outcomes (AdjOR = 1.73, p < 0.001). As for distal variables, less affluent adolescents were the most affected, and males reported fewer emotional problems than females. Uncertainty regarding the disease in a context of socioeconomic vulnerability, together with rises in unhealthy behaviors and isolation from their immediate social circles, have negatively affected adolescents’ emotional status throughout the COVID-19 pandemic.
Purification Efficiency of Three Combinations of Native Aquatic Macrophytes in Artificial Wastewater in Autumn
Authors: Xu Lei , Chen Siyu , Zhuang Ping , Xie Dongsheng , Yu Xiaoling , Liu Dongming , Li Zhian , Qin Xinsheng , Wang Faguo , Xing Fuwu
Published: June 2021

Water pollution caused by excessive nutrient and biological invasion is increasingly widespread in China, which can lead to problems with drinking water as well as serious damage to the ecosystem if not be properly treated. Aquatic plant restoration (phytoremediation) has become a promising and increasingly popular solution. In this study, eight native species of low-temperature-tolerant aquatic macrophytes were chosen to construct three combinations of aquatic macrophytes to study their purification efficiency on eutrophic water in large open tanks during autumn in Guangzhou City. The total nitrogen (TN) removal rates of group A (Vallisneria natans + Ludwigia adscendens + Monochoria vaginalis + Saururus chinensis), group B (V. natans + Ipomoea aquatica + Acorus calamus + Typha orientalis), and group C (V. natans + L. adscendens + Schoenoplectus juncoides + T. orientalis) were 79.10%, 46.39%, and 67.46%, respectively. The total phosphorus (TP) removal rates were 89.39%, 88.37%, and 91.96% in groups A, B, and C, respectively, while the chemical oxygen demand (COD) removal rates were 93.91%, 96.48%, and 92.78%, respectively. In the control group (CK), the removal rates of TN, TP, and COD were 70.42%, 86.59%, and 87.94%, respectively. The overall removal rates of TN, TP, and COD in the plant groups were only slightly higher than that in CK group, which did not show a significant advantage. This may be related to the leaf decay of some aquatic plants during the experiment, whereby the decay of V. natans was the most obvious. The results suggest that a proper amount of plant residue will not lead to a significant deterioration of water quality.
Comparison of ARIMA and LSTM in Forecasting the Incidence of HFMD Combined and Uncombined with Exogenous Meteorological Variables in Ningbo, China
Authors: Zhang Rui , Guo Zhen , Meng Yujie , Wang Songwang , Li Shaoqiong , Niu Ran , Wang Yu , Guo Qing , Li Yonghong
Published: June 2021

Background: This study intends to identify the best model for predicting the incidence of hand, foot and mouth disease (HFMD) in Ningbo by comparing Autoregressive Integrated Moving Average (ARIMA) and Long Short-Term Memory Neural Network (LSTM) models combined and uncombined with exogenous meteorological variables. Methods: The data of daily HFMD incidence in Ningbo from January 2014 to November 2017 were set as the training set, and the data of December 2017 were set as the test set. ARIMA and LSTM models combined and uncombined with exogenous meteorological variables were adopted to fit the daily incidence of HFMD by using the data of the training set. The forecasting performances of the four fitted models were verified by using the data of the test set. Root mean square error (RMSE) was selected as the main measure to evaluate the performance of the models. Results: The RMSE for multivariate LSTM, univariate LSTM, ARIMA and ARIMAX (Autoregressive Integrated Moving Average Model with Exogenous Input Variables) was 10.78, 11.20, 12.43 and 14.73, respectively. The LSTM model with exogenous meteorological variables has the best performance among the four models and meteorological variables can increase the prediction accuracy of LSTM model. For the ARIMA model, exogenous meteorological variables did not increase the prediction accuracy but became the interference factor of the model. Conclusions: Multivariate LSTM is the best among the four models to fit the daily incidence of HFMD in Ningbo. It can provide a scientific method to build the HFMD early warning system and the methodology can also be applied to other communicable diseases.
Neuroinflammation as a Common Denominator of Complex Diseases (Cancer, Diabetes Type 2, and Neuropsychiatric Disorders)
Authors: Asslih Serena , Damri Odeya , Agam Galila
Published: June 2021

The term neuroinflammation refers to inflammation of the nervous tissue, in general, and in the central nervous system (CNS), in particular. It is a driver of neurotoxicity, it is detrimental, and implies that glial cell activation happens prior to neuronal degeneration and, possibly, even causes it. The inflammation-like glial responses may be initiated in response to a variety of cues such as infection, traumatic brain injury, toxic metabolites, or autoimmunity. The inflammatory response of activated microglia engages the immune system and initiates tissue repair. Through translational research the role played by neuroinflammation has been acknowledged in different disease entities. Intriguingly, these entities include both those directly related to the CNS (commonly designated neuropsychiatric disorders) and those not directly related to the CNS (e.g., cancer and diabetes type 2). Interestingly, all the above-mentioned entities belong to the same group of “complex disorders”. This review aims to summarize cumulated data supporting the hypothesis that neuroinflammation is a common denominator of a wide variety of complex diseases. We will concentrate on cancer, type 2 diabetes (T2DM), and neuropsychiatric disorders (focusing on mood disorders).
Immunodiagnostic Biomarkers for Hepatocellular Carcinoma (HCC): The First Step in Detection and Treatment
Authors: Xing Mengtao , Wang Xinzhi , Kirken Robert , He Ling , Zhang Jian-Ying
Published: June 2021

Hepatocellular carcinoma (HCC) exerts huge effects on the health burden of the world because of its high mortality and poor prognosis. HCC is often clinically detected late in patients. If HCC could be detected and treated earlier, the survival rate of patients will be greatly improved. Therefore, identifying specific biomarkers is urgent and important for HCC. The liver is also recognized as an immune organ. The occurrence of HCC is related to exacerbation of immune tolerance and/or immunosurveillance escape. The host immune system plays an important role in the recognition and targeting of tumor cells in cancer immunotherapy, as can be seen from the clinical success of immune checkpoint inhibitors and chimeric antigen receptor (CAR) T cells. Thus, there is a pressing medical need to discover immunodiagnostic biomarkers specific to HCC for understanding the pathological mechanisms of HCC, especially for immunotherapy targets. We have reviewed the existing literature to summarize the immunodiagnostic markers of HCC, including autoantibodies against tumor-associated antigens (TAAs) and exosomes, to provide new insights into HCC and early detection of this deadly cancer.
Influence of Recycled Aggregates on the Mechanical Properties of Synthetic Fibers-Reinforced Masonry Mortars
Authors: Morón Alberto , Ferrández Daniel , Saiz Pablo , Vega Gabriela , Morón Carlos
Published: June 2021

The construction sector is one of the main consumers of raw materials and generates a high volume of waste within the European Union. The search for new materials that are more sustainable and respectful of the environment has become a challenge for countries with a high degree of industrialization. In this work, a study of the most relevant properties of masonry mortars made with recycled aggregates and reinforced with synthetic fibers was carried out. Three types of aggregates were used—natural, concrete recycling and ceramic recycling—and two types of reinforcing fibers: polypropylene and polyolefin. In this way, various tests of physical-mechanical characterization and a statistical analysis of the results were carried out. It has been shown that the mortars made from aggregate recycled concrete and reinforced with polypropylene fiber are the ones with the best properties for application in the construction sector, although without improving the properties of traditional mortars made from natural aggregate and without fibers.
Understanding Traffic Congestion via Network Analysis, Agent Modeling, and the Trajectory of Urban Expansion: A Coastal City Case
Authors: Amézquita-López Julio , Valdés-Atencio Jorge , Angulo-García David
Published: June 2021

The study of patterns of urban mobility is of utter importance for city growth projection and development planning. In this paper, we analyze the topological aspects of the street network of the coastal city of Cartagena de Indias employing graph theory and spatial syntax tools. We find that the resulting network can be understood on the basis of 400 years of the city’s history and its peripheral location that strongly influenced and shaped the growth of the city, and that the statistical properties of the network resemble those of self-organized cities. Moreover, we study the mobility through the network using a simple agent-based model that allows us to study the level of street congestion depending on the agents’ knowledge of the traffic while they travel through the network. We found that a purely shortest-path travel scheme is not an optimal strategy and that assigning small weights to traffic avoidance schemes increases the overall performance of the agents in terms of arrival success, occupancy of the streets, and traffic accumulation. Finally, we argue that localized congestion can be only partially ascribed to topological properties of the network and that it is important to consider the decision-making capability of the agents while moving through the network to explain the emergence of traffic congestion in the system.
Short-Term Selection to Diflubenzuron and Bacillus thuringiensis Var. Israelensis Differentially Affects the Winter Survival of Culex pipiens f. Pipiens and Culex pipiens f. Molestus (Diptera: Culicidae)
Authors: Ioannou Charalampos S. , Hadjichristodoulou Christos , Kyritsi Maria A. , Papadopoulos Nikos T.
Published: June 2021

The Culex pipiens (Diptera: Culicidae) mosquito is of high medical importance as it is considered the prime vector of West Nile virus. In Europe, this species consists of two forms, named pipiens and molestus, that exhibit substantial differences in their overwintering biology. Diflubenzuron (DFB) and Bacillus thuringiensis var. israelensis (Bti) are two of the most used larvicides in mosquito control, including that of Culex pipiens. The high dependency on these two larvicides poses major concerns for resistance development. The evolution and stability of resistance to insecticides has been associated with fitness costs that may be manifested under stressful conditions, such as the winter period. This study investigated how short-term selection of pipiens and molestus forms to both larvicides affect their overwintering success. Larvae from each form were subjected to the same selective pressure (80% mortality) for three successive generations with DFB and Bti. At the end of this process, the winter survival between the selected populations and the controls (colonies without selection) was determined for each form. Selection to both larvicides significantly reduced the winter survival rates of molestus but not of pipiens form, indicating potential differences in the persistence of the selected individuals from year to year between the two forms.
Factors Influencing the Reproductive Ability of Male Bees: Current Knowledge and Further Directions
Authors: Zhao Huiyue , Mashilingi Shibonage K. , Liu Yanjie , An Jiandong
Published: June 2021

Bumblebees and honeybees are very important pollinators and play a vital role in agricultural and natural ecosystems. The quality of their colonies is determined by the queens and the reproductive drones of mother colonies, and mated drones transmit semen, including half of the genetic materials, to queens and enhance their fertility. Therefore, factors affecting drone fecundity will also directly affect progeny at the colony level. Here, we review environmental and bee-related factors that are closely related to drone reproductive ability. The environmental factors that mainly affect the sperm count and the viability of males include temperature, nutrients and pesticides. In addition, the inherent characteristics of male bees, such as body size, weight, age, seminal fluid proteins and proteins of the spermathecal fluid, contribute to mating success, sperm quality during long-term storage in the spermathecae and the reproductive behaviors of queens. Based on the results of previous studies, we also suggest that the effects of somatotype dimorphism in bumblebee males on sperm quality and queen fecundity and the indispensable and exploitable function of gland proteins in the fecundity of males and queens should be given more attention in further studies.
Functional Response and Predation Rate of Dicyphus cerastii Wagner (Hemiptera: Miridae)
Authors: Abraços-Duarte Gonçalo , Ramos Susana , Valente Fernanda , Borges da Silva Elsa , Figueiredo Elisabete
Published: June 2021

Dicyphine mirids are important biological control agents (BCAs) in horticultural crops. Dicyphus cerastii Wagner can be found in protected tomato crops in Portugal, and has been observed feeding on several tomato pests. However, the predation capacity of this species is poorly studied. In order to investigate the predation capacity of D. cerastii, and how it is affected by prey size and mobility, we evaluated the functional response (FR) and predation rate of female predators on different densities of four prey species: Myzus persicae 1st instar nymphs (large mobile prey), Bemisia tabaci 4th instar nymphs, Ephestia kuehniella eggs (large immobile prey) and Tuta absoluta eggs (small immobile prey). Experiments were performed on tomato leaflets in Petri dish arenas for 24 h. Dicyphus cerastii exhibited type II FR for all prey tested. The predator effectively preyed upon all prey, consuming an average of 88.8 B. tabaci nymphs, 134.4 E. kuehniella eggs, 37.3 M. persicae nymphs and 172.3 T. absoluta eggs. Differences in the FR parameters, attack rate and handling time, suggested that prey size and mobility affected predation capacity. Considering the very high predation rates found for all prey species, D. cerastii proved to be an interesting candidate BCA for tomato crops.