bluuebunny/crossref_metadata_2025 huggingface dataset:
STAT3 in acute myeloid leukemia facilitates natural killer cell-mediated surveillance
Authors: Witalisz-Siepracka Agnieszka , Denk Clio-Melina , Zdársky Bernhard , Hofmann Lorenz , Edtmayer Sophie , Harm Theresa , Weiss Stefanie , Heindl Kerstin , Hessenberger Manuel , Summer Sabrina , Dutta Sayantanee , Casanova Emilio , Obermair Gerald J. , Győrffy Balázs , Putz Eva Maria , Sill Heinz , Stoiber Dagmar
Published: July 2024

Acute myeloid leukemia (AML) is a heterogenous disease characterized by the clonal expansion of myeloid progenitor cells. Despite recent advancements in the treatment of AML, relapse still remains a significant challenge, necessitating the development of innovative therapies to eliminate minimal residual disease. One promising approach to address these unmet clinical needs is natural killer (NK) cell immunotherapy. To implement such treatments effectively, it is vital to comprehend how AML cells escape the NK-cell surveillance. Signal transducer and activator of transcription 3 (STAT3), a component of the Janus kinase (JAK)-STAT signaling pathway, is well-known for its role in driving immune evasion in various cancer types. Nevertheless, the specific function of STAT3 in AML cell escape from NK cells has not been deeply investigated. In this study, we unravel a novel role of STAT3 in sensitizing AML cells to NK-cell surveillance. We demonstrate that STAT3-deficient AML cell lines are inefficiently eliminated by NK cells. Mechanistically, AML cells lacking STAT3 fail to form an immune synapse as efficiently as their wild-type counterparts due to significantly reduced surface expression of intercellular adhesion molecule 1 (ICAM-1). The impaired killing of STAT3-deficient cells can be rescued by ICAM-1 overexpression proving its central role in the observed phenotype. Importantly, analysis of our AML patient cohort revealed a positive correlation between ICAM1 and STAT3 expression suggesting a predominant role of STAT3 in ICAM-1 regulation in this disease. In line, high ICAM1 expression correlates with better survival of AML patients underscoring the translational relevance of our findings. Taken together, our data unveil a novel role of STAT3 in preventing AML cells from escaping NK-cell surveillance and highlight the STAT3/ICAM-1 axis as a potential biomarker for NK-cell therapies in AML.
Mature tertiary lymphoid structures: important contributors to anti-tumor immune efficacy
Authors: Bao Xinyu , Lin Xuwen , Xie Mei , Yao Jie , Song Jialin , Ma Xidong , Zhang Xin , Zhang Yinguang , Liu Yiming , Han Wenya , Liang Yiran , Hu Hongling , Xu Li , Xue Xinying
Published: July 2024

Tertiary lymphoid structures (TLS) represent the ectopic aggregations of immune cells arising during chronic inflammation or tumor progression. In cancer, TLS are often associated with beneficial clinical outcomes in patients undergoing immunotherapy, underscoring their prognostic and predictive significance. Mature TLS, characterized by germinal centers and areas of T-cell and B-cell aggregation, are considered primary locations for activating and maintaining both humoral and cellular anti-tumor immune effects. Despite their recognized importance, the mechanisms driving the formation of mature TLS in cancer and their influence on the immune response within tumors remain insufficiently understood. Therefore, this review aims to comprehensively explore the structural composition, development mechanisms, maturity impact factors, immunological function, and innovative therapeutic strategies of mature TLS within the tumor microenvironment. The research summarized herein offers novel insights and considerations for therapeutic approaches to promote TLS generation and maturation in patients with cancer, representing a promising avenue for future cancer therapies.
Evaluation of damage performance in offshore floating photovoltaics-based hydrogen production system due to potential hydrogen release
Authors: Wang Hao , Wang Kan , Liu Xiaolei , Liu Yang , Qian Zhijia , Ding Sheng
Published: July 2024

Green hydrogen is an important future energy source, which offers a vast potential to implement the decarbonization of the marine sector and advance broad shift to clean-energy alternatives globally. There are various advantages of offshore floating photovoltaics (FPVs) technology for hydrogen production; however, hydrogen storage in FPVs-based hydrogen production system faces several challenges. It is found that the major barrier concerning the system under investigation is related to safety. The current study aims to present an applicable offshore FPVs-based hydrogen production system, which involves both the FPV section and the hydrogen production section based on a project in China. A numerical 3D model is performed to investigate the characteristics of accidental damage through potential hydrogen storage device failure during system operation. The hydrogen release process of an FPVs-based hydrogen production system is presented with different offshore wind conditions, and the parameters for understanding the motion state and hydrogen release mode of hydrogen are also analyzed. The study further explores the dynamic development of hydrogen dispersion from a hydrogen production platform, including a momentum-dominated region, a horizontal spreading region, and a vertical buoyancy region. In addition, the influence of hydrogen explosive flame on thermal damage evaluation is illustrated, and thermal hazards under different offshore wind conditions are also discussed. The current study contributes to a better understanding of failure analysis of the FPVs–hydrogen production system and elaborates on damage evolution of hydrogen storage integrated with the system. The study also concentrates on marine environmental synergistic limits considering thermally damaged mechanical properties.
The utility of software-detected non-invasive tear break-up in comparison to fluorescein tear break-up measurements
Authors: Pflugfelder Stephen C. , Kikukawa Yasushi , Tanaka Shin , Kosugi Takuya
Published: July 2024

Purpose

The purpose of this study is to characterize and discuss the difference between software-detected non-invasive tear break-up time (NIBUT) and the traditional clinical method of fluorescein break-up time (FBUT).

Methods

Tear interferometry with the KOWA DR-1α (Kowa, Japan) and a standardized comprehensive ocular surface/tear evaluation were performed in 307 eyes. Software-detected NIBUT in the KOWA DR-1α images and the investigator-detected FBUT were compared.

Results

Software-detected NIBUT was significantly shorter than investigator-measured FBUT. NIBUT was 3.1 ± 2.5 s (mean ± SD), whereas FBUT was 4.8 ± 3.0 s. This difference was due to three different patterns or conditions: a spot break immediately after eyelid opening, moderate to severe keratitis sicca, and epithelial basement membrane corneal dystrophy (EBMD). In these cases, rapid tear film disruption was not captured by FBUT. A spot break immediately after eye opening that rapidly disappears was observed with conjunctivochalasis. This type of break-up may be difficult to detect using fluorescein because the human eye cannot catch such rapid blinks or post-blink events. In the second group with severe corneal epithelial disease, break-up may occur over the entire corneal surface upon eye opening, and distinct fluorescein tear break-up may not be identified because of poor dye dilution or spread over the corneal surface, whereas the non-invasive break-up is not solution-dependent, and the software can detect a distinct appearance. In the third group with EBMD, it is possible that focal break-up in the fluorescein pattern over the epithelial elevations, which might be missed visually, can be detected by software in video images.

Conclusion

We found that software-detected NIBUT is more sensitive in detecting tear break-up, can identify certain tear film disruptions that are missed by traditional FBUT, and may be more useful in distinguishing certain tear disorders.
Extensively and multidrug-resistant bacterial strains: case studies of antibiotics resistance
Authors: Almutairy Bandar
Published: July 2024

The development of antibiotic resistance compromises the effectiveness of our most effective defenses against bacterial infections, presenting a threat to global health. To date, a large number of research articles exist in the literature describing the case reports associated with extensively drug-resistant (XDR) and multidrug-resistant (MDR) bacterial strains. However, these findings are scattered, making it time-consuming for researchers to locate promising results and there remains a need for a comparative study to compile these case reports from various geographical regions including the Kingdom of Saudi Arabia. Additionally, no study has yet been published that compares the genetic variations and case reports of MDR and XDR strains identified from Saudi Arabia, the Middle East, Central Europe, and Asian countries. This study attempts to provide a comparative analysis of several MDR and XDR case reports from Saudi Arabia alongside other countries. Furthermore, the purpose of this work is to demonstrate the genetic variations in the genes underlying the resistance mechanisms seen in MDR and XDR bacterial strains that have been reported in Saudi Arabia and other countries. To cover the gap, this comprehensive review explores the complex trends in antibiotic resistance and the growing risk posed by superbugs. We provide context on the concerning spread of drug-resistant bacteria by analyzing the fundamental mechanisms of antibiotic resistance and looking into individual case reports. In this article, we compiled various cases and stories associated with XDR and MDR strains from Saudi Arabia and various other countries including China, Egypt, India, Poland, Pakistan, and Taiwan. This review will serve as basis for highlighting the growing threat of MDR, XDR bacterial strains in Saudi Arabia, and poses the urgent need for national action plans, stewardship programs, preventive measures, and novel antibiotics research in the Kingdom.
Microstimulation reveals anesthetic state-dependent effective connectivity of neurons in cerebral cortex
Authors: Hudetz Anthony G
Published: July 2024

Introduction

Complex neuronal interactions underlie cortical information processing that can be compromised in altered states of consciousness. Here intracortical microstimulation was applied to investigate anesthetic state-dependent effective connectivity of neurons in rat visual cortex in vivo.

Methods

Extracellular activity was recorded at 32 sites in layers 5/6 while stimulating with charge-balanced discrete pulses at each electrode in random order. The same stimulation pattern was applied at three levels of anesthesia with desflurane and in wakefulness. Spikes were sorted and classified by their waveform features as putative excitatory and inhibitory neurons. Network motifs were identified in graphs of effective connectivity constructed from monosynaptic cross-correlograms.

Results

Microstimulation caused early (<10 ms) increase followed by prolonged (11–100 ms) decrease in spiking of all neurons throughout the electrode array. The early response of excitatory but not inhibitory neurons decayed rapidly with distance from the stimulation site over 1 mm. Effective connectivity of neurons with significant stimulus response was dense in wakefulness and sparse under anesthesia. The number of network motifs, especially those of higher order, increased rapidly as the anesthesia was withdrawn indicating a substantial increase in network connectivity as the animals woke up.

Conclusion

The results illuminate the impact of anesthesia on functional integrity of local cortical circuits affecting the state of consciousness.
Association between ultra-processed dietary pattern and bullying: the role of deviant behaviors
Authors: Okada Letícia Martins , Marques Emanuele Souza , Levy Renata Bertazzi , Gonçalves Vivian Siqueira Santos , Peres Maria Fernanda Tourinho , Azeredo Catarina Machado
Published: July 2024

Background

Ultra-processed foods have been associated with several negative outcomes, but it is not clear whether they are related to bullying perpetration. Moreover, no previous study has investigated the potential role of deviant behaviors as a mediator of this association. Our objective was to evaluate the association between ultra-processed dietary pattern and bullying, and the mediating effect of deviant behaviors in this association, among school adolescents.

Methods

We used data from a representative sample of 9th grade Brazilian adolescents (N = 2,212) from the São Paulo Project for the social development of children and adolescents (SP-PROSO). Exploratory factor analysis was used to obtain the dietary patterns, through questions of frequency of consumption in the last week of several foods. The ultra-processed dietary pattern was considered as exposure. The outcomes were the types of bullying (any type, social exclusion, psychological/verbal aggression, physical aggression, property destruction, and sexual harassment). Deviant behaviors (mediator) were assessed through a score. Mediation analyses were carried out using logistic regression based on the KHB method.

Results

After adjusting for covariates, the mediating effect of deviant behaviors was found in the association between ultra-processed dietary pattern and all the types of bullying perpetration, especially for psychological/verbal aggression (39.4%). A small mediating effect of deviant behaviors in the association of ultra-processed dietary pattern with physical aggression (17.7%) and property destruction (18.5%) was observed, but this effect explained only a small portion of the total effect of such association (significant direct effect).

Conclusion

The ultra-processed dietary pattern was associated with bullying, and the association was mediated through deviant behaviors. Policies and actions for improving the adolescent’s diet and managing the adoption of deviant and bullying behaviors by this public are required.
Case report: A case study of neoadjuvant immunochemotherapy for locally advanced esophageal squamous carcinoma
Authors: Liu Xiong , Wang Maoqi , Meng Deyuan , Tang Yuntao , Shi Qingtong
Published: July 2024

In locally advanced esophageal cancer, the controversy over the two traditional treatment modalities, neoadjuvant radiotherapy and neoadjuvant chemotherapy, has been unending and also challenged by the addition of neoadjuvant immunotherapy. Neoadjuvant immunotherapy has led to an increasing diversity of neoadjuvant combination treatment modalities, among which neoadjuvant immunochemotherapy has emerged, with current clinical studies initially demonstrating its efficacy and safety. We report a case of a patient with locally advanced esophageal cancer who underwent two cycles of neoadjuvant immunochemotherapy and successful surgery and achieved a pathological complete response (pCR). A 73-year-old elderly female patient presented with progressive dysphagia for more than 1 month with an Eastern Cooperative Oncology Group (ECOG) score of 1. After completing gastroscopy + pathological biopsy, chest enhanced CT, barium esophageal meal, PET-CT, and other related examinations, the clinical diagnosis was thoracic segmental esophageal poorly differentiated squamous carcinoma cT2N2M0 stage III. After a multidisciplinary discussion of the comprehensive treatment plan, two cycles of neoadjuvant therapy were given on February 16, 2023, and March 9, 2023, and the treatment plan was as follows: cisplatin 30 mg d1–3 + albumin paclitaxel 200 mg d1 and 100 mg d8 + sintilimab 200 mg d4, q3w. After the neoadjuvant therapy, the patient underwent an imaging examination. The chest enhanced CT suggested that the lesion range was significantly reduced compared with the previous scan, and mediastinal lymph nodes were partially reduced. Then, thoracoscopic radical esophageal cancer surgery was performed on April 23, 2023. pCR was achieved by pathological evaluation, and the postoperative diagnosis was thoracic segmental esophageal hypofractionated squamous carcinoma ypT0N0M0. Two cycles of adjuvant immunochemotherapy were performed after surgery on May 30, 2023, and June 21, 2023, and the regimen was as follows: cisplatin 30 mg d1–3 + albumin paclitaxel 200 mg d1 and 100 mg d8 + sindilizumab 200 mg d4, q3w. As of the latest review on March 20, 2024, the patient was not seen to have any significant postoperative complications and remains in a state of complete response (CR). Neoadjuvant immunochemotherapy has positive significance for the treatment of patients with locally advanced esophageal cancer. Whether neoadjuvant immunochemotherapy can replace neoadjuvant synchronous radiotherapy is a future direction of research, which needs to be further verified by more reliable clinical trials.
Nomogram for predicting overall survival of metastatic pancreatic cancer patients based on HBV infection and inflammatory-nutritional biomarkers
Authors: Long Xiawei , Li Qian , Liao Sina , Lin Youzhi , Liao Xiaoli
Published: July 2024

Purpose

To develop and validate a nomogram for predicting the overall survival of patients with metastatic pancreatic cancer.

Methods

This retrospective study included 236 patients with metastatic pancreatic cancer treated at Guangxi Medical University Cancer Hospital between October 2013 and October 2022. Patients were grouped according to hepatitis B virus (HBV) infection status. Cox proportional hazard regression was used to identify the prognostic factors independently associated with overall survival. Results were used to build a nomogram, which was assessed through internal validation using bootstrap resampling.

Results

Patients in the HBV-positive group (N = 37) showed significantly better overall survival than those in the HBV-negative group (N=199; P = 0.014). Overall survival was independently associated with the following factors: HBV infection status, sex, chemotherapy, metastatic sites, a combined index of hemoglobin, albumin, lymphocytes, and platelets, neutrophil-albumin ratio, as well as levels of CA125. The nomogram showed good predictive power, with an area under the curve of 0.808 for the time-dependent receiver operating characteristic. Calibration and decision curve analyses indicated good calibration and clinical usefulness of the nomogram for predicting the overall survival of patients with metastatic pancreatic cancer.

Conclusion

A nomogram based on the HBV infection status and inflammatory nutritional markers may help predict the overall survival of patients with metastatic pancreatic cancer and guide personalized clinical treatment.
Immunotherapy and radiotherapy for older patients with invasive bladder cancer unfit for surgery or chemotherapy: practical proposal by the international geriatric radiotherapy group
Authors: Nguyen Nam Phong , Karlsson Ulf Lennart , Page Brandi R. , Chirila Monica-Emilia , Vinh-Hung Vincent , Gorobets Olena , Arenas Meritxell , Mohammadianpanah Mohammad , Javadinia Seyed Alireza , Giap Huan , Kim Lyndon , Dutheil Fabien , Murthy Vedang , Mallum Abba Aji , Tlili Ghassen , Dahbi Zineb , Loganadane Gokoulakrichenane , Blanco Sergio Calleja , Bose Satya , Natoli Elena , Li Eric , Morganti Alessio G.
Published: July 2024

The standard of care for non-metastatic muscle invasive bladder cancer is either radical cystectomy or bladder preservation therapy, which consists of maximal transurethral bladder resection of the tumor followed by concurrent chemoradiation with a cisplatin-based regimen. However, for older cancer patients who are too frail for surgical resection or have decreased renal function, radiotherapy alone may offer palliation. Recently, immunotherapy with immune checkpoint inhibitors (ICI) has emerged as a promising treatment when combined with radiotherapy due to the synergy of those two modalities. Transitional carcinoma of the bladder is traditionally a model for immunotherapy with an excellent response to Bacille Calmette-Guerin (BCG) in early disease stages, and with avelumab and atezolizumab for metastatic disease. Thus, we propose an algorithm combining immunotherapy and radiotherapy for older patients with locally advanced muscle-invasive bladder cancer who are not candidates for cisplatin-based chemotherapy and surgery.
The functions and mechanisms of long non-coding RNA in colorectal cancer
Authors: Lin Yuning , Zhao Wenzhen , Lv Zhenyi , Xie Hongyan , Li Ying , Zhang Zhongying
Published: July 2024

CRC poses a significant challenge in the global health domain, with a high number of deaths attributed to this disease annually. If CRC is detected only in its advanced stages, the difficulty of treatment increases significantly. Therefore, biomarkers for the early detection of CRC play a crucial role in improving patient outcomes and increasing survival rates. The development of a reliable biomarker for early detection of CRC is particularly important for timely diagnosis and treatment. However, current methods for CRC detection, such as endoscopic examination, blood, and stool tests, have certain limitations and often only detect cases in the late stages. To overcome these constraints, researchers have turned their attention to molecular biomarkers, which are considered a promising approach to improving CRC detection. Non-invasive methods using biomarkers such as mRNA, circulating cell-free DNA, microRNA, LncRNA, and proteins can provide more reliable diagnostic information. These biomarkers can be found in blood, tissue, stool, and volatile organic compounds. Identifying molecular biomarkers with high sensitivity and specificity for the early and safe, economic, and easily measurable detection of CRC remains a significant challenge for researchers.
Evaluation of the combined predictive value of multiple indicators based on diaphragmatic ultrasound using logistic regression and ROC curve in weaning from mechanical ventilation in pediatric patients
Authors: Ge Hejia , Zhang Ailian , Teng Yiqun , Hu Li
Published: July 2024

Background

Conventional single indicators have low sensitivity and specificity for predicting weaning from mechanical ventilation in pediatric patients, necessitating the establishment of a combined prediction model for predicting weaning outcomes.

Objectives

To explore the combined predictive value of PaO2/FiO2 Ratio (P/F ratio), diaphragm excursion-rapid shallow breathing index (DE-RSBI), diaphragm thickening fraction-rapid shallow breathing index (DTF-RSBI), and Pediatric Critical Illness Score (PCIS) in weaning from mechanical ventilation in pediatric patients.

Methods

Sixty critically ill pneumonia pediatric patients requiring mechanical ventilation treatment from July 2022 to June 2023 at the Second Affiliated Hospital of Jiaxing University were selected. They all underwent a spontaneous breathing trial (SBT) and were divided into the weaning success group (42 cases) and weaning failure group (18 cases) based on the weaning outcome. Parameters including total duration of illness, mechanical ventilation duration, heart rate (HR), P/F ratio, diaphragm excursion (DE), DE-RSBI, diaphragm thickening fraction (DTF), DTF-RSBI, and PCIS were included in univariate and multivariate logistic regression analyses to determine independent factors affecting pediatric weaning success. Receiver operating characteristic (ROC) curves were plotted to evaluate the predictive value of P/F ratio, DE-RSBI, DTF-RSBI, PCIS alone or in combination for weaning success.

Results

Comparing P/F ratio, DE, DE-RSBI, DTF, DTF-RSBI and PCIS, there were statistically significant differences (P < 0.05). Through collinearity analysis and binary logistic regression analysis,P/F ratio [OR = 0.777, 95% CI (0.641,0.941)], DE-RSBI [OR = 1.694, 95% CI (1.172, 2.447)], DTF-RSBI [OR = 1.057, 95% CI (1.002, 1.114)], and PCIS [OR = 0.661, 95% CI (0.445, 0.982)] were identified as independent factors affecting successful weaning(P < 0.05).The regression equation was: LogitP = 73.299–0.253 P/F ratio + 0.525DE-RSBI + 0.055DTF-RSBI-0.43PCIS.The sensitivity of the combined indicator Logit(P) in predicting successful weaning from mechanical ventilation in pediatric patients was 88.9%, with a specificity of 95.2% (optimal cutoff value of 0.511), and the area under the ROC curve (AUC) was 0.960 [95% CI (0.915, 1.000)]. The AUC of the combined prediction model for predicting pediatric weaning was greater than that of P/F ratio, DE-RSBI, DTF-RSBI and PCIS alone (Z values = 9.129, 2.061, 2.075, 8.326, P < 0.05).

Conclusions

In mechanically ventilated pediatric patients, the combined prediction model has better predictive value for weaning success compared to using P/F ratio, DE-RSBI, DTF-RSBI, or PCIS alone.
Multisystem inflammatory syndrome in neonates (MIS-N): an updated systematic review
Authors: Muthiah Divya , Chan Ming , Low Yue Wey , Ramasamy Sheena Nishanti , Amin Zubair , Chan-Ng Pauline Poh Lin , Low Jeen Liang , Low Jia Ming
Published: July 2024

Introduction

The aim of the study was to summarize and update clinical features and outcomes of multisystem inflammatory syndrome in neonates (MIS-N).

Methods

A systematic literature search was conducted of studies on MIS-N published in PubMed, MEDLINE, EMBASE, CNKI, and WHO COVID-19 databases between 1 December 2019 and 30 June 2023. Reference lists of selected articles, Google Scholar, and pre-print servers were searched for additional studies. The methodological quality of included studies was assessed.

Results

Of 1,572 records screened after the initial search, 35 studies involving a total of 201 neonates with MIS-N were included. One study was retrieved from a pre-print server. For those with available data, 34/47 (78.7%) mothers were infected in the third trimester. Of the 199 mothers (two with twin pregnancies), 183 (92.0%) were from India. The median age of neonates at presentation was 2.0 days (interquartile range 1.0–9.5). Over two-thirds (144/201, 71.6%) presented with respiratory distress, while 112 (55.7%) had cardiac involvement, such as ventricular dysfunctions, involvement of coronary arteries, and atrioventricular blocks. Arrhythmias and thrombosis were reported in 15/201 (7.5%) and 2/201 (3.0%) neonates, respectively. All neonates, except one, required critical care; 64/160 (40.0%) required inotropic support and 105/187 (56.1%) required respiratory support, of whom 59/105 (56.2%) were specified to require intubation. The mortality rate was 5.0% (10/201).

Discussion/Conclusion

MIS-N should be considered in ill neonates presenting with involvement of two or more organ systems, especially among those neonates with cardiorespiratory dysfunctions, in the presence of proven or suspected maternal COVID-19 infection during pregnancy.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021278717, PROSPERO, identifier CRD42021278717.
Long Mu Qing Xin mixture improves behavioral performance in spontaneously hypertensive rats (SHR/NCrl) by upregulating catecholamine neurotransmitters in prefrontal cortex and striatum via DRD1/cAMP/PKA-CREB signaling pathway
Authors: Li Xuejun , Xiao Zhen , Jiang Zhiyan , Pu Wenyan , Chen Xiufeng , Wang Shumin , Liu Anqi , Zhang Hongyu , Xu Zihao
Published: July 2024

Background

Attention deficit hyperactivity disorder (ADHD), a prevalent neurodevelopmental disorder in children, can be effectively alleviated by the herbal preparation Long Mu Qing Xin Mixture (LMQXM), but its mechanism has not been fully elucidated.

Objective

To scrutinize the potential pharmacological mechanisms by which LMQXM improves behavior in spontaneously hypertensive rats (SHR/NCrl).

Methods

The SHR/NCrl rats were randomly stratified into the model (SHR) group, the methylphenidate hydrochloride (MPH) group, and groups subjected to varying dosages of LMQXM into the medium dose (MD) group with a clinically effective dose, the low dose (LD) group with 0.5 times the clinically effective dose, and high dose (HD) group with 2 times the clinically effective dose. Furthermore, the WKY/NCrl rats constituted the control group. The evaluation of behavior involved the open field test and the Morris water maze test. HPLC, LC-MS, ELISA, immunohistochemistry, Western blot, and RT-qPCR were utilized to scrutinize the catecholamine neurotransmitter content and the expression of proteins and genes associated with the dopamine receptor D1 (DRD1)/cAMP/protein kinase A (PKA)-cAMP response element-binding (CREB) pathway in prefrontal cortex (PFC) and striatum.

Results

MPH and LMQXM ameliorated hyperactivity and learning and memory deficits of SHR/NCrl rats. Among them, LMQXM-MD and MPH also upregulated dopamine (DA), norepinephrine (NE), adenylate cyclase (AC) and cAMP levels, and the expression of proteins and genes associated with the DRD1/cAMP/PKA-CREB pathway in PFC and striatum of SHR/NCrl rats. PFC and striatum DA levels were also upregulated in the LMQXM-LD group as well as the striatum DA levels in the LMQXM-HD group, but there were no statistically significant differences in their NE levels compared to the SHR group. LMQXM-LD and LMQXM-HD also upregulated some DRD1/cAMP/PKA-CREB pathway-related proteins and gene expression, but the effects were discernibly disparate in PFC and striatum. Upon comprehensive analysis, LMQXM-MD appeared to be the most effective dose.

Conclusion

Our study tentatively suggests that LMQXM may rectify hyperactivity and learning and memory deficits of SHR/NCrl rats by elevating catecholamine neurotransmitters in the PFC and striatum. This effect may be attributed to the potential activation of the DRD1/cAMP/PKA-CREB signaling pathway, which appears to achieve an optimal response at moderate doses.
Network pharmacology: an efficient but underutilized approach in oral, head and neck cancer therapy—a review
Authors: Muthuramalingam Pandiyan , Jeyasri Rajendran , Varadharajan Venkatramanan , Priya Arumugam , Dhanapal Anand Raj , Shin Hyunsuk , Thiruvengadam Muthu , Ramesh Manikandan , Krishnan Murugesan , Omosimua Rebecca Oziohu , Sathyaseelan Divyan Devasir , Venkidasamy Baskar
Published: July 2024

The application of network pharmacology (NP) has advanced our understanding of the complex molecular mechanisms underlying diseases, including neck, head, and oral cancers, as well as thyroid carcinoma. This review aimed to explore the therapeutic potential of natural network pharmacology using compounds and traditional Chinese medicines for combating these malignancies. NP serves as a pivotal tool that provides a comprehensive view of the interactions among compounds, genes, and diseases, thereby contributing to the advancement of disease treatment and management. In parallel, this review discusses the significance of publicly accessible databases in the identification of oral, head, and neck cancer-specific genes. These databases, including those for head and neck oral cancer, head and neck cancer, oral cancer, and genomic variants of oral cancer, offer valuable insights into the genes, miRNAs, drugs, and genetic variations associated with these cancers. They serve as indispensable resources for researchers, clinicians, and drug developers, contributing to the pursuit of precision medicine and improved treatment of these challenging malignancies. In summary, advancements in NP could improve the globalization and modernization of traditional medicines and prognostic targets as well as aid in the development of innovative drugs. Furthermore, this review will be an eye-opener for researchers working on drug development from traditional medicines by applying NP approaches.
Exploration of chemical compositions in different germplasm wolfberry using UPLC-MS/MS and evaluation of the in vitro anti-inflammatory activity of quercetin
Authors: Lan Tian , Duan Guozhen , Qi Youchao , Almezgagi Maged , Fan Guanghui , Ma Yonggui
Published: July 2024

Wolfberry, esteemed as a traditional Chinese medicinal material and functional food, is replete with nutrients and boasts a diverse array of health benefits, including hypoglycemic, antitumor, antioxidant, anti-inflammatory, and immune-enhancing properties. Notably, inflammation is a pivotal factor in the onset and progression of numerous diseases. Despite this, there is a paucity of research on the comprehensive evaluation of the components found in different wolfberries, and the exploration of their primary active components is limited. To address this issue, we conducted a comprehensive targeted metabolomics analysis, employing statistical methods such as principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA), KEGG pathway analysis, and volcano plots to delineate the compositional differences among red, black, and yellow wolfberries. Furthermore, we investigated the anti-inflammatory effects of their primary components through in vitro experiments. Our analysis revealed a total of 1,104 chemical compositions in the three wolfberries, with alkaloids, phenolic acids, flavonoids, and lipids being the predominant nutritional components. KEGG enrichment analysis indicated that these compositions were primarily involved in the biosynthesis of secondary metabolites, ABC transport, and galactose metabolism pathway. Moreover, our study demonstrated that quercetin exhibited dose-dependent anti-inflammatory activity in LPS-stimulated HUVECs. It effectively inhibited the production of inflammatory factors such as TNF-α, MCP-1, and IL-1β, while also down-regulating the gene and protein expression levels of ICAM-1 and VCAM-1. In conclusion, our findings indicate that there are variations in compositions among the three wolfberries, with flavonoids being the most abundant, and in vitro studies also confirmed the anti-inflammatory potential of quercetin. It is worth noting that Lycium ruthenicum contains higher levels of antioxidant components and possesses greater nutritional value, providing valuable insights for the future development and utilization of the three wolfberries.
Spatial effects analysis of natural forest canopy cover based on spaceborne LiDAR and geostatistics
Authors: Yu Jinge , Xu Li , Shu Qingtai , Luo Shaolong , Xi Lei
Published: July 2024

Because of the high cost of manual surveys, the analysis of spatial change of forest structure at the regional scale faces a difficult challenge. Spaceborne LiDAR can provide global scale sampling and observation. Taking this opportunity, dense natural forest canopy cover (NFCC) observations obtained by combining spaceborne LiDAR data, plot survey, and machine learning algorithm were used as spatial attributes to analyze the spatial effects of NFCC. Specifically, based on ATL08 (Land and Vegetation Height) product generated from Ice, Cloud and land Elevation Satellite-2/Advanced Topographic Laser Altimeter System (ICESat-2/ATLAS) data and 80 measured plots, the NFCC values located at the LiDAR’s footprint locations were predicted by the ML model. Based on the predicted NFCC, the spatial effects of NFCC were analyzed by Moran’s I and semi-variogram. The results showed that (1) the Random Forest (RF) model had the strongest predicted performance among the built ML models (R2=0.75, RMSE=0.09); (2) the NFCC had a positive spatial correlation (Moran’s I = 0.36), that is, the CC of adjacent natural forest footprints had similar trends or values, belonged to the spatial agglomeration distribution; the spatial variation was described by the exponential model (C0 = 0.12×10-2, C = 0.77×10-2, A0 = 10200 m); (3) topographic factors had significant effects on NFCC, among which elevation was the largest, slope was the second, and aspect was the least; (4) the NFCC spatial distribution obtained by SGCS was in great agreement with the footprint NFCC (R2 = 0.59). The predictions generated from the RF model constructed using ATL08 data offer a dependable data source for the spatial effects analysis.
Deciphering the maize gene ZmGF14–3: implications for plant height based on co-expression networks
Authors: Wang Hengsheng , Wei Bo , Qi Lulu , Chen Yansong , Chen Kelong , Liu Dong , Su Xu , Zhang Yan , Li Lingling
Published: July 2024

The evolutionary analysis showed that the GF14 family was conserved, however, there was limited evidence linking GF14s to plant height. In our investigations, we discovered a co-expression relationship between ZmGF14s and functionally characterized genes linked to plant height. In the co-expression network, we identified ZmGF14-3, a gene expression exhibiting a positive correlation with plant height in three maize varieties, we postulated that this gene could be intimately linked to plant height development. Subsequently, we cloned ZmGF14-3 from the maize B73 inbred line and overexpressed it in Arabidopsis, resulting in markedly dwarfed transgenic phenotypes. Measurements of endogenous phytohormones disclosed a significant reduction in concentrations of Gibberellic Acid 7 (GA7) and Indole-3-Acetic Acid (IAA) in the overexpressed Arabidopsis, furthermore, qPCR results highlighted a pronounced decrease in the expression levels of plant height-related genes when compared to the wild type, therefore, it is plausible to posit that ZmGF14-3 plays a pivotal role in regulating the growth and development of maize through interactions with various phytohormone-related genes. Thus, delving into the potential interactions between ZmGF14-3 and these genes holds the promise of yielding valuable insights into the molecular mechanisms underpinning plant height development in maize.
Polychlorinated biphenyls modify Arabidopsis root exudation pattern to accommodate degrading bacteria, showing strain and functional trait specificity
Authors: Rolli Eleonora , Ghitti Elisa , Mapelli Francesca , Borin Sara
Published: July 2024

Introduction

The importance of plant rhizodeposition to sustain microbial growth and induce xenobiotic degradation in polluted environments is increasingly recognized.

Methods

Here the “cry-for-help” hypothesis, consisting in root chemistry remodeling upon stress, was investigated in the presence of polychlorinated biphenyls (PCBs), highly recalcitrant and phytotoxic compounds, highlighting its role in reshaping the nutritional and signaling features of the root niche to accommodate PCB-degrading microorganisms.

Results

Arabidopsis exposure to 70 µM PCB-18 triggered plant-detrimental effects, stress-related traits, and PCB-responsive gene expression, reproducing PCB phytotoxicity. The root exudates of plantlets exposed for 2 days to the pollutant were collected and characterized through untargeted metabolomics analysis by liquid chromatography–mass spectrometry. Principal component analysis disclosed a different root exudation fingerprint in PCB-18-exposed plants, potentially contributing to the “cry-for-help” event. To investigate this aspect, the five compounds identified in the exudate metabolomic analysis (i.e., scopoletin, N-hydroxyethyl-β-alanine, hypoxanthine, L-arginyl-L-valine, and L-seryl-L-phenylalanine) were assayed for their influence on the physiology and functionality of the PCB-degrading strains Pseudomonas alcaliphila JAB1, Paraburkholderia xenovorans LB400, and Acinetobacter calcoaceticus P320. Scopoletin, whose relative abundance decreased in PCB-18-stressed plant exudates, hampered the growth and proliferation of strains JAB1 and P320, presumably due to its antimicrobial activity, and reduced the beneficial effect of Acinetobacter P320, which showed a higher degree of growth promotion in the scopoletin-depleted mutant f6’h1 compared to Arabidopsis WT plants exposed to PCB. Nevertheless, scopoletin induced the expression of the bph catabolic operon in strains JAB1 and LB400. The primary metabolites hypoxanthine, L-arginyl-L-valine, and L-seryl-L-phenylalanine, which increased in relative abundance upon PCB-18 stress, were preferentially used as nutrients and growth-stimulating factors by the three degrading strains and showed a variable ability to affect rhizocompetence traits like motility and biofilm formation.

Discussion

These findings expand the knowledge on PCB-triggered “cry-for-help” and its role in steering the PCB-degrading microbiome to boost the holobiont fitness in polluted environments.
Parenting styles and personal belief in a just world among Chinese children and adolescents: gender, living location, and age as moderators
Authors: Wang Jing , Ye Yonghong , Wang Yun , Zeng Xihua
Published: July 2024

Introduction

The positive development of the personal belief in a just world (PBJW) plays a vital role in academic achievement and mental health among children and adolescents. Therefore, it is necessary to understand the influencing factors of PBJW better. The association between parenting styles and PBJW has been well established, but whether this association varies among different groups remains an open issue. The present study aimed to examine the strength of the associations between parenting styles and PBJW among Chinese children and adolescents and the role of certain moderators (gender, living location, and age) in these associations.

Methods

This study employed hierarchical regression and simple slopes analyses to examine data from the National Children's Study of China. The database includes 24,013 Chinese children and adolescents in grades 49 (M = 12.76 years, SD = 1.73), with 53.50% boys.

Results

The results indicated that (1) authoritative parenting was positively correlated with PBJW; (2) both authoritarian and permissive parenting styles were negatively correlated with PBJW; (3) the positive relationship between authoritative parenting and PBJW was more prominent in urban regions; the negative relationship between authoritarian and PBJW was stronger in urban regions; and the negative relationship between permissive parenting and PBJW was more pronounced among girls and older children and adolescents.

Discussion

These findings highlight important associations between parenting styles and the development of PBJW among Chinese children and adolescents, and suggest strategies for policy-makers, educators, and parents to improve PBJW for different types of Chinese children and adolescents.
The neurocognitive processing mechanism of English subject-verb agreement by Chinese-speaking learners
Authors: Wu Mingjun , Li Miaomiao , Wu Di
Published: July 2024

Determiner phrases (DPs), an overarching term, can be classified into two determiner types: referential determiner phrases (RDPs, e.g., the boy) and quantificational determiner phrases (QDPs, e.g., each boy). Using the event-related potential (ERP) technique, this study explored the modulation of RDP vs. QDP in the online processing of English subject–verb agreement with omission errors by Chinese learners of English, addressing the question of whether singular quantification increases or decreases Chinese learners’ sensitivity to agreement violations. The experiment manipulated the determiner type, specifically RDP vs. QDP, and grammaticality (grammatical vs. ungrammatical). The results indicated that similar to previous studies, a P600 effect was elicited in response to subject–verb agreement violations with omission errors, demonstrating that Chinese L2 learners are sensitive to such agreement violations. Additionally, the ERP patterns exhibited variations due to D-linking and number specification of RDP and QDP. Regarding D-linking, subject–verb agreement violations in the QDP conditions, necessitating integration of discourse-related knowledge, elicited laterally and frontally distributed P600 effects associated with integration complexity at the discourse level; however, non-D-linked referential determiners elicited the posteriorly-distributed P600 effects. Differences in number specification resulted in the distinctive P600 latencies and whether P600 was preceded by N400 or not. While both the RDP and QDP conditions exhibited the P600 effects, the onset latency of this effect in the number-unspecified RDP condition was 300 ms later compared to the number-specified QDP condition. Furthermore, an additional N400 component observed in the RDP condition suggests that L2 learners acquire morphologically complex subject–verb agreements by rote, treating them as unanalyzed chunks. This N400 component was absent in the QDP condition. From these results, the conclusion can be drawn that L2 learners are sensitive to the subject–verb agreement violations with omission errors, and L2 processing patterns of subject–verb agreement vary with different features of determiners, providing further evidence for the cue-based retrieval model during comprehension of grammatical sentences. Pedagogical implications are provided, and the future research direction is suggested.
Social prescribing for suicide prevention: a rapid review
Authors: Dash Sarah , McNamara Stella , de Courten Maximilian , Calder Rosemary
Published: July 2024

This rapid review delves into the realm of social prescribing as a novel approach to suicide prevention by addressing the social determinants of health. Through an exploration of various databases including MEDLINE, PsychInfo, WILEY, and Sage, a total of 3,063 articles were initially identified as potentially relevant to the research. Following a meticulous screening process, 13 articles were included in the final review, shedding light on the potential effectiveness and impact of social prescribing interventions on suicide prevention. Key findings indicate the need for additional monitoring and support for individuals at risk of suicide, emphasising warm referrals and sustained connections after referral to enhance the efficacy of social prescribing models. The review also highlights the importance of social capital and trust among vulnerable populations, underscoring the significance of community-based referrals in suicide prevention initiatives. Overall, this review identifies the potential of social prescribing as a valuable tool in mitigating suicide risk factors and promoting mental health and wellbeing in diverse populations.
The relationship between sleep quality and occupational fatigue in endoscopy nurses: mediating role of positive coping style
Authors: Zeng Zhi , Zhou Sumei , Xie Guiqiong , He Yazhi , Ling Jing
Published: July 2024

Background

Nursing occupational fatigue has emerged as a critical issue affecting the safety and health of nurses. This phenomenon not only impairs nurses’ performance and mental well-being but also poses risks to patient safety and the quality of care provided. This study focuses on endoscopic nurses to explore the mediating role of positive coping styles between sleep quality and occupational fatigue, aiming to identify effective strategies to alleviate fatigue, thereby improving the work environment and enhancing healthcare quality.

Methods

From July to August 2023, a cross-sectional design was used to select 258 endoscopy nurses from 25 top-three hospitals in 14 cities across 5 provinces in China. Data was collected through general information questionnaires, Fatigue assessment instrument, Pittsburgh sleep quality index, and Simple Coping Style Questionnaire. A structural equation model of sleep quality – positive coping style – occupational fatigue was constructed using Amos 26.0, and Bootstrap was employed to test the mediating effect.

Results

The results showed that the mean scores of sleep quality, occupational fatigue, and positive coping style for endoscopy nurses were 8.89 ± 4.13, 17.73 ± 5.64, and 18.32 ± 10.46, respectively. Positive coping style were negatively correlated with sleep quality and occupational fatigue (p < 0.001). Positive coping style partially mediated the relationship between sleep quality and occupational fatigue, with a mediating effect value of 0.253, accounting for 42.10% of the total effect.

Conclusion

Sleep quality can indirectly affect the level of occupational fatigue through positive coping style. Nursing managers should enhance nurses’ positive coping skills, improve nurses’ sleep quality, and reduce occupational fatigue among nurses.