bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of the Degree of Inversion on the Photoelectrochemical Activity of Spinel ZnFe2O4
Authors: Granone Luis I. , Nikitin Konstantin , Emeline Alexei , Dillert Ralf , Bahnemann Detlef W.
Published: May 2019

Physicochemical properties of spinel ZnFe2O4 (ZFO) are known to be strongly affected by the distribution of the cations within the oxygen lattice. In this work, the correlation between the degree of inversion, the electronic transitions, the work function, and the photoelectrochemical activity of ZFO was investigated. By room-temperature photoluminescence measurements, three electronic transitions at approximately 625, 547, and 464 nm (1.98, 2.27, and 2.67 eV, respectively) were observed for the samples with different cation distributions. The transitions at 625 and 547 nm were assigned to near-band-edge electron-hole recombination processes involving O2- 2p and Fe3+ 3d levels. The transition at 464 nm, which has a longer lifetime, was assigned to the relaxation of the excited states produced after electron excitations from O2- 2p to Zn2+ 4s levels. Thus, under illumination with wavelengths shorter than 464 nm, electron-hole pairs are produced in ZFO by two apparently independent mechanisms. Furthermore, the charge carriers generated by the O2− 2p to Zn2+ 4s electronic transition at 464 nm were found to have a higher incident photon-to-current efficiency than the ones generated by the O2− 2p to Fe3+ 3d electronic transition. As the degree of inversion of ZFO increases, the probability of a transition involving the Zn2+ 4s levels increases and the probability of a transition involving the Fe3+ 3d levels decreases. This effect contributes to the increase in the photoelectrochemical efficiency observed for the ZFO photoanodes having a larger cation distribution.
Onset of Telomere Dysfunction and Fusions in Human Ovarian Carcinoma
Authors: Huda Nazmul , Xu Yan , Bates Alison M. , Rankin Deborah A. , Kannan Nagarajan , Gilley David
Published: May 2019

Telomere dysfunction has been strongly implicated in the initiation of genomic instability and is suspected to be an early event in the carcinogenesis of human solid tumors. Recent findings have established the presence of telomere fusions in human breast and prostate malignancies; however, the onset of this genomic instability mechanism during progression of other solid cancers is not well understood. Herein, we explored telomere dynamics in patient-derived epithelial ovarian cancers (OC), a malignancy characterized by multiple distinct subtypes, extensive molecular heterogeneity, and widespread genomic instability. We discovered a high frequency of telomere fusions in ovarian tumor tissues; however, limited telomere fusions were detected in normal adjacent tissues or benign ovarian samples. In addition, we found relatively high levels of both telomerase activity and hTERT expression, along with anaphase bridges in tumor tissues, which were notably absent in adjacent normal ovarian tissues and benign lesions. These results suggest that telomere dysfunction may occur early in ovarian carcinogenesis and, importantly, that it may play a critical role in the initiation and progression of the disease. Recognizing telomere dysfunction as a pervasive feature of this heterogeneous malignancy may facilitate the future development of novel diagnostic tools and improved methods of disease monitoring and treatment.
Kallikrein 12 Regulates Innate Resistance of Murine Macrophages against Mycobacterium bovis Infection by Modulating Autophagy and Apoptosis
Authors: Sabir Naveed , Hussain Tariq , Liao Yi , Wang Jie , Song Yinjuan , Shahid Muhammad , Cheng Guangyu , Mangi Mazhar Hussain , Yao Jiao , Yang Lifeng , Zhao Deming , Zhou Xiangmei
Published: May 2019

Mycobacterium bovis (M. bovis) is a member of the Mycobacterium tuberculosis (Mtb) complex causing bovine tuberculosis (TB) and imposing a high zoonotic threat to human health. Kallikreins (KLKs) belong to a subgroup of secreted serine proteases. As their role is established in various physiological and pathological processes, it is likely that KLKs expression may mediate a host immune response against the M. bovis infection. In the current study, we report in vivo and in vitro upregulation of KLK12 in the M. bovis infection. To define the role of KLK12 in immune response regulation of murine macrophages, we produced KLK12 knockdown bone marrow derived macrophages (BMDMs) by using siRNA transfection. Interestingly, the knockdown of KLK12 resulted in a significant downregulation of autophagy and apoptosis in M. bovis infected BMDMs. Furthermore, we demonstrated that this KLK12 mediated regulation of autophagy and apoptosis involves mTOR/AMPK/TSC2 and BAX/Bcl-2/Cytochrome c/Caspase 3 pathways, respectively. Similarly, inflammatory cytokines IL-1β, IL-6, IL-12 and TNF-α were significantly downregulated in KLK12 knockdown macrophages but the difference in IL-10 and IFN-β expression was non-significant. Taken together, these findings suggest that upregulation of KLK12 in M. bovis infected murine macrophages plays a substantial role in the protective immune response regulation by modulating autophagy, apoptosis and pro-inflammatory pathways. To our knowledge, this is the first report on expression and the role of KLK12 in the M. bovis infection and the data may contribute to a new paradigm for diagnosis and treatment of bovine TB.
Systematic Review of Patient-Derived Xenograft Models for Preclinical Studies of Anti-Cancer Drugs in Solid Tumors
Authors: Koga Yoshikatsu , Ochiai Atsushi
Published: May 2019

Patient-derived xenograft (PDX) models are used as powerful tools for understanding cancer biology in PDX clinical trials and co-clinical trials. In this systematic review, we focus on PDX clinical trials or co-clinical trials for drug development in solid tumors and summarize the utility of PDX models in the development of anti-cancer drugs, as well as the challenges involved in this approach, following the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. Recently, the assessment of drug efficacy by PDX clinical and co-clinical trials has become an important method. PDX clinical trials can be used for the development of anti-cancer drugs before clinical trials, with their efficacy assessed by the modified response evaluation criteria in solid tumors (mRECIST). A few dozen cases of PDX models have completed enrollment, and the efficacy of the drugs is assessed by 1 × 1 × 1 or 3 × 1 × 1 approaches in the PDX clinical trials. Furthermore, co-clinical trials can be used for personalized care or precision medicine with the evaluation of a new drug or a novel combination. Several PDX models from patients in clinical trials have been used to assess the efficacy of individual drugs or drug combinations in co-clinical trials.
Nucleolar Structure and Function in Trypanosomatid Protozoa
Authors: Martínez-Calvillo Santiago , Florencio-Martínez Luis E. , Nepomuceno-Mejía Tomás
Published: May 2019

The nucleolus is the conspicuous nuclear body where ribosomal RNA genes are transcribed by RNA polymerase I, pre-ribosomal RNA is processed, and ribosomal subunits are assembled. Other important functions have been attributed to the nucleolus over the years. Here we review the current knowledge about the structure and function of the nucleolus in the trypanosomatid parasites Trypanosoma brucei, Trypanosoma cruzi and Leishmania ssp., which represent one of the earliest branching lineages among the eukaryotes. These protozoan parasites present a single nucleolus that is preserved throughout the closed nuclear division, and that seems to lack fibrillar centers. Trypanosomatids possess a relatively low number of rRNA genes, which encode rRNA molecules that contain large expansion segments, including several that are trypanosomatid-specific. Notably, the large subunit rRNA (28S-type) is fragmented into two large and four small rRNA species. Hence, compared to other organisms, the rRNA primary transcript requires additional processing steps in trypanosomatids. Accordingly, this group of parasites contains the highest number ever reported of snoRNAs that participate in rRNA processing. The number of modified rRNA nucleotides in trypanosomatids is also higher than in other organisms. Regarding the structure and biogenesis of the ribosomes, recent cryo-electron microscopy analyses have revealed several trypanosomatid-specific features that are discussed here. Additional functions of the nucleolus in trypanosomatids are also reviewed.
Membrane Protein Identification in Rodent Brain Tissue Samples and Acute Brain Slices
Authors: Joost Sarah , Mikkat Stefan , Wille Michael , Schümann Antje , Schmitt Oliver
Published: May 2019

Acute brain slices are a sample format for electrophysiology, disease modeling, and organotypic cultures. Proteome analyses based on mass spectrometric measurements are seldom used on acute slices, although they offer high-content protein analyses and explorative approaches. In neuroscience, membrane proteins are of special interest for proteome-based analysis as they are necessary for metabolic, electrical, and signaling functions, including myelin maintenance and regeneration. A previously published protocol for the enrichment of plasma membrane proteins based on aqueous two-phase polymer systems followed by mass spectrometric protein identification was adjusted to the small sample size of single acute murine slices from newborn animals and the reproducibility of the results was analyzed. For this, plasma membrane proteins of 12 acute slice samples from six animals were enriched and analyzed by liquid chromatography-mass spectrometry. A total of 1161 proteins were identified, of which 369 were assigned to membranes. Protein abundances showed high reproducibility between samples. The plasma membrane protein separation protocol can be applied to single acute slices despite the low sample size and offers a high yield of identifiable proteins. This is not only the prerequisite for proteome analysis of organotypic slice cultures but also allows for the analysis of small-sized isolated brain regions at the proteome level.
Sonic Hedgehog Ligand: A Role in Formation of a Mesenchymal Niche in Human Pancreatic Ductal Adenocarcinoma
Authors: Saini Francesca , Argent Richard H. , Grabowska Anna M.
Published: May 2019

Pancreatic ductal adenocarcinoma (PDAC) is characterised by desmoplasia, thought to support progression and chemotherapeutic resistance. The Hedgehog pathway is known to play an important role in this cancer. While the upregulation of Sonic hedgehog (Shh) in the epithelium of PDAC is known, we investigated its expression in the tumour microenvironment in order to find new targets for new chemotherapeutical approaches. Immunohistochemistry was used for the investigation of Shh and Vimentin in primary human pancreatic tissues. Gene (qRT-PCR) and protein (immunofluorescence) expression of Shh, αSMA (a marker of the mesenchymal phenotype) and periostin (a marker of mesenchymal cells within a mixed population) were investigated in in vitro cell models. Shh expression was significantly upregulated in the stromal and epithelial compartments of poorly-differentiated PDAC samples, with a strong correlation with the amount of stroma present. Characterisation of stromal cells showed that there was expression of Shh ligand in a mixed population comprising αSMA+ myofibroblasts and αSMA− mesenchymal stem cells. Moreover, we demonstrated the interaction between these cell lines by showing a higher rate of mesenchymal cell proliferation and the upregulation of periostin. Therefore, targeting stromal Shh could affect the equilibrium of the tumour microenvironment and its contribution to tumour growth.
Characterisation of Osteopontin in an In Vitro Model of Embryo Implantation
Authors: Berneau Stéphane C , Ruane Peter T , Brison Daniel R , Kimber Susan J , Westwood Melissa , Aplin John D
Published: May 2019

At the onset of pregnancy, embryo implantation is initiated by interactions between the endometrial epithelium and the outer trophectoderm cells of the blastocyst. Osteopontin (OPN) is expressed in the endometrium and is implicated in attachment and signalling roles at the embryo–epithelium interface. We have characterised OPN in the human endometrial epithelial Ishikawa cell line using three different monoclonal antibodies, revealing at least nine distinct molecular weight forms and a novel secretory pathway localisation in the apical domain induced by cell organisation into a confluent epithelial layer. Mouse blastocysts co-cultured with Ishikawa cell layers served to model embryo apposition, attachment and initial invasion at implantation. Exogenous OPN attenuated initial, weak embryo attachment to Ishikawa cells but did not affect the attainment of stable attachment. Notably, exogenous OPN inhibited embryonic invasion of the underlying cell layer, and this corresponded with altered expression of transcription factors associated with differentiation from trophectoderm (Gata2) to invasive trophoblast giant cells (Hand1). These data demonstrate the complexity of endometrial OPN forms and suggest that OPN regulates embryonic invasion at implantation by signalling to the trophectoderm.
Mitochondrial DNA, a Powerful Tool to Decipher Ancient Human Civilization from Domestication to Music, and to Uncover Historical Murder Cases
Authors: Merheb Maxime , Matar Rachel , Hodeify Rawad , Siddiqui Shoib Sarwar , Vazhappilly Cijo George , Marton John , Azharuddin Syed , AL Zouabi Hussain
Published: May 2019

Mitochondria are unique organelles carrying their own genetic material, independent from that in the nucleus. This review will discuss the nature of mitochondrial DNA (mtDNA) and its levels in the cell, which are the key elements to consider when trying to achieve molecular identification in ancient and degraded samples. mtDNA sequence analysis has been appropriately validated and is a consistent molecular target for the examination of biological evidence encountered in forensic cases—and profiling, in certain conditions—especially for burnt bodies and degraded samples of all types. Exceptional cases and samples will be discussed in this review, such as mtDNA from leather in Beethoven’s grand piano, mtDNA in mummies, and solving famous historical criminal cases. In addition, this review will be discussing the use of ancient mtDNA to understand past human diet, to trace historical civilizations and ancient trade routes, and to uncover geographical domestication origins and lineage relationships. In each topic, we will present the power of mtDNA and how, in many cases, no nuclear DNA was left, leaving mitochondrial DNA analysis as a powerful alternative. Exploring this powerful tool further will be extremely useful to modern science and researchers, due to its capabilities in providing us with previously unattainable knowledge.
Evolutionary Divergence of Duplicated Hsf Genes in Populus
Authors: Liu Bobin , Hu Jianjun , Zhang Jin
Published: May 2019

Heat shock transcription factors (Hsfs), which function as the activator of heat shock proteins (Hsps), play multiple roles in response to environmental stress and the development of plants. The Hsf family had experienced gene expansion via whole-genome duplication from a single cell algae to higher plants. However, how the Hsf gene family went through evolutionary divergence after genome duplication is unknown. As a model wood species, Populus trichocarpa is widely distributed in North America with various ecological and climatic environments. In this study, we used P. trichocarpa as materials and identified the expression divergence of the PtHsf gene family in developmental processes, such as dormant bud formation and opening, catkins development, and in response to environments. Through the co-expression network, we further discovered the divergent co-expressed genes that related to the functional divergence of PtHsfs. Then, we studied the alternative splicing events, single nucleotide polymorphism distribution and tertiary structures of members of the PtHsf gene family. In addition to expression divergence, we uncovered the evolutionary divergence in the protein level which may be important to new function formations and for survival in changing environments. This study comprehensively analyzed the evolutionary divergence of a member of the PtHsf gene family after genome duplication, paving the way for further gene function analysis and genetic engineering.
2-Oxonanonoidal Antibiotic Actinolactomycin Inhibits Cancer Progression by Suppressing HIF-1α
Authors: Cheng Jiadong , Hu Lan , Yang Zheng , Suo Caixia , Wang Yueyang Jack , Gao Ping , Cui Chengbin , Sun Linchong
Published: May 2019

HIF-1 serves as an important regulator in cell response to hypoxia. Due to its key role in promoting tumor survival and progression under hypoxia, HIF-1 has become a promising target of cancer therapy. Thus far, several HIF-1 inhibitors have been identified, most of which are from synthesized chemical compounds. Here, we report that ALM (ActinoLactoMycin), a compound extracted from metabolites of Streptomyces flavoretus, exhibits inhibitory effect on HIF-1α. Mechanistically, we found that ALM inhibited the translation of HIF-1α protein by suppressing mTOR signaling activity. Treatment with ALM induced cell apoptosis and growth inhibition of cancer cells both in vitro and in vivo in a HIF-1 dependent manner. More interestingly, low dose of ALM treatment enhanced the anti-tumor effect of Everolimus, an inhibitor of mTOR, suggesting its potential use in combination therapy of tumors, especially solid tumor patients. Thus, we identified a novel HIF-1α inhibitor from the metabolites of Streptomyces flavoretus, which shows promising anti-cancer potential.
Patient Derived Chicken Egg Tumor Model (PDcE Model): Current Status and Critical Issues
Authors: Komatsu Aoi , Matsumoto Kotaro , Saito Tomoki , Muto Manabu , Tamanoi Fuyuhiko
Published: May 2019

Chorioallantoic membrane assay (CAM assay) using fertilized chicken eggs has been used for the study of tumor formation, angiogenesis and metastasis. Recently, there is growing realization that this system provides a valuable assay for a patient-derived tumor model. Several reports establish that tumor samples from cancer patients can be used to reproduce tumor in the chicken egg. High transplantation efficiency has been achieved. In this review, we discuss examples of transplanting patient tumors. We then discuss critical issues that need to be addressed to pursue this line of experiments. The patient-derived chicken egg model (PDcE model) has an advantage over other models in its rapid tumor formation. This raises the possibility that the PDcE model is valuable for identifying optimum drug for each individual patient.
Systemic Dysfunction of Osteoblast Differentiation in Adipose-Derived Stem Cells from Patients with Multiple Myeloma
Authors: Béréziat Véronique , Mazurier Christelle , Auclair Martine , Ferrand Nathalie , Jolly Séverine , Marie Tiffany , Kobari Ladan , Toillon Indira , Delhommeau François , Fève Bruno , Larsen Annette K. , Sabbah Michèle , Garderet Laurent
Published: May 2019

Multiple myeloma is characterized by bone lesions linked to increased osteoclast and decreased osteoblast activities. In particular, the osteoblast differentiation of bone marrow-derived stem cells (MSC) is impaired. Among the potential therapeutic tools for counteracting bone lesions, adipose-derived stem cells (ASC) could represent an appealing source for regenerative medicine due to their similar characteristics with MSC. Our study is among the first giving detailed insights into the osteoblastogenic capacities of ASC isolated by fat aspiration from myeloma patients (MM-ASC) compared to healthy subjects (HD-ASC). We showed that MM-ASC and HD-ASC exhibited comparable morphology, proliferative capacity, and immunophenotype. Unexpectedly, although normal in adipocyte differentiation, MM-ASC present a defective osteoblast differentiation, as indicated by less calcium deposition, decreased alkaline phosphatase activity, and downregulation of RUNX2 and osteocalcin. Furthermore, these ASC-derived osteoblasts displayed enhanced senescence, as shown by an increased β-galactosidase activity and cell cycle inhibitors expression (p16INK4A, p21WAF1/CIP1.), associated with a markedly increased expression of DKK1, a major inhibitor of osteoblastogenesis in multiple myeloma. Interestingly, inhibition of DKK1 attenuated senescence and rescued osteoblast differentiation, highlighting its key role. Our findings show, for the first time, that multiple myeloma is a systemic disease and suggest that ASC from patients would be unsuitable for tissue engineering designed to treat myeloma-associated bone disease.
Physiological Adaptation to Simultaneous Chronic Exposure to High-Fat Diet and Dichlorodipheniletylhene (DDE) in Wistar Rat Testis
Authors: Migliaccio Vincenzo , Sica Raffaella , Scudiero Rosaria , Simoniello Palma , Putti Rosalba , Lionetti Lillà
Published: May 2019

Environmental chemicals can be introduced by consuming contaminated foods. The environmental chemical dichlorodiphenyldichloroethylene (DDE), a persistent metabolite of dichlorodiphenyltrichloroethane (DDT), can affect spermatogenesis. Our study aims to evaluate, by using spectrophotometric analyses, western blot, and immunohistochemistry, the adaptive responses in testis of adult rats treated with a non-toxic dose of DDE, alone or in association with a high-fat diet (HFD). Four experimental groups were performed: N (normal diet); D (HFD); D + DDE (HFD + DDE); N + DDE (normal diet + DDE). D group showed a reduction in antioxidant capacity, and increases in lipid peroxidation, apoptosis, and proliferation associated with morphological impairment. A reduction in androgen receptor (AR) and serum testosterone levels were also found. DDE-treated groups exhibited higher lipid peroxidation levels compared to N and D, associated with pronounced defect in antioxidant capacity, apoptosis, cellular proliferation, as well as with tissue damage. Moreover, decreases in AR and serum testosterone levels were found in DDE-treated groups vs. N and D. In conclusion, HFD and DDE produced cellular stress leading to antioxidant impairment, apoptosis, and decreases in AR and serum testosterone levels associated with tissue damage. Cellular proliferation could be used as an adaptation to counterbalance the occurred damage, maintaining a pool of tubules that follow physiological maturation.
Strategies to Interfere with Tumor Metabolism through the Interplay of Innate and Adaptive Immunity
Authors: Mora Javier , Mertens Christina , Meier Julia K. , Fuhrmann Dominik C. , Brüne Bernhard , Jung Michaela
Published: May 2019

The inflammatory tumor microenvironment is an important regulator of carcinogenesis. Tumor-infiltrating immune cells promote each step of tumor development, exerting crucial functions from initiation, early neovascularization, to metastasis. During tumor outgrowth, tumor-associated immune cells, including myeloid cells and lymphocytes, acquire a tumor-supportive, anti-inflammatory phenotype due to their interaction with tumor cells. Microenvironmental cues such as inflammation and hypoxia are mainly responsible for creating a tumor-supportive niche. Moreover, it is becoming apparent that the availability of iron within the tumor not only affects tumor growth and survival, but also the polarization of infiltrating immune cells. The interaction of tumor cells and infiltrating immune cells is multifaceted and complex, finally leading to different activation phenotypes of infiltrating immune cells regarding their functional heterogeneity and plasticity. In recent years, it was discovered that these phenotypes are mainly implicated in defining tumor outcome. Here, we discuss the role of the metabolic activation of both tumor cells and infiltrating immune cells in order to adapt their metabolism during tumor growth. Additionally, we address the role of iron availability and the hypoxic conditioning of the tumor with regard to tumor growth and we describe the relevance of therapeutic strategies to target such metabolic characteristics.
Collagen Peptide Upregulates Osteoblastogenesis from Bone Marrow Mesenchymal Stem Cells through MAPK- Runx2
Authors: Elango Jeevithan , Robinson Jeyashakila , Zhang Jingyi , Bao Bin , Ma Nan , de Val José Eduardo Maté Sánchez , Wu Wenhui
Published: May 2019

Collagen is the most abundant extracellular fibrous protein that has been widely used for biomedical applications due to its excellent biochemical and biocompatibility features. It is believed that the smaller molecular weight collagen, i.e., collagen peptide (CP), has more potent activity than native collagen. However, the preparation of CP from fish bone collagen is a complex and time-consuming process. Additionally, the osteogenic effect of CP depends on its molecular weight and amino acid composition. Considering the above concept, the present work was undertaken to extract the CP directly from Mahi mahi fish (Coryphaena hippurus) bones and test its osteogenic potential using bone marrow mesenchymal stem (BMMS) cells. The hydrolyzed collagen contained triple alpha chains (110 kDa) and a peptide (~1 kDa) and the peptide was successfully separated from hydrolyzed collagen using molecular weight cut-off membrane. CP treatment was up-regulated BMMS cells proliferation and differentiation. Interestingly, CP accrued the mineral deposition in differentiated BMMS cells. Protein and mRNA expression revealed that the osteogenic biomarkers such as collagen, alkaline phosphatase, and osteocalcin levels were significantly increased by CP treatment in differentiated BMMS cells and also further elucidated the hypothesis that CP was upregulated osteogenesis through activating Runx2 via p38MAPK signaling pathway. The above results concluded that the CP from Mahi mahi bones with excellent osteogenic properties could be the suitable biomaterial for bone therapeutic application.
Protective Effects of Licochalcone A Ameliorates Obesity and Non-Alcoholic Fatty Liver Disease Via Promotion of the Sirt-1/AMPK Pathway in Mice Fed a High-Fat Diet
Authors: Liou Chian-Jiun , Lee Yau-Ker , Ting Nai-Chun , Chen Ya-Ling , Shen Szu-Chuan , Wu Shu-Ju , Huang Wen-Chung
Published: May 2019

Licochalcone A is a chalcone isolated from Glycyrrhiza uralensis. It showed anti-tumor and anti-inflammatory properties in mice with acute lung injuries and regulated lipid metabolism through the activation of AMP-activated protein kinase (AMPK) in hepatocytes. However, the effects of licochalcone A on reducing weight gain and improving nonalcoholic fatty liver disease (NAFLD) are unclear. Thus, the present study investigated whether licochalcone A ameliorated weight loss and lipid metabolism in the liver of high-fat diet (HFD)-induced obese mice. Male C57BL/6 mice were fed an HFD to induce obesity and NAFLD, and then were injected intraperitoneally with licochalcone A. In another experiment, a fatty liver cell model was established by incubating HepG2 hepatocytes with oleic acid and treating the cells with licochalcone A to evaluate lipid metabolism. Our results demonstrated that HFD-induced obese mice treated with licochalcone A had decreased body weight as well as inguinal and epididymal adipose tissue weights compared with HFD-treated mice. Licochalcone A also ameliorated hepatocyte steatosis and decreased liver tissue weight and lipid droplet accumulation in liver tissue. We also found that licochalcone A significantly regulated serum triglycerides, low-density lipoprotein, and free fatty acids, and decreased the fasting blood glucose value. Furthermore, in vivo and in vitro, licochalcone A significantly decreased expression of the transcription factor of lipogenesis and fatty acid synthase. Licochalcone A activated the sirt-1/AMPK pathway to reduce fatty acid chain synthesis and increased lipolysis and β-oxidation in hepatocytes. Licochalcone A can potentially ameliorate obesity and NAFLD in mice via activation of the sirt1/AMPK pathway.
The B-Side of Cancer Immunity: The Underrated Tune
Authors: Largeot Anne , Pagano Giulia , Gonder Susanne , Moussay Etienne , Paggetti Jerome
Published: May 2019

Tumor-infiltrating lymphocytes are known to be critical in controlling tumor progression. While the role of T lymphocytes has been extensively studied, the function of B cells in this context is still ill-defined. In this review, we propose to explore the role of B cells in tumor immunity. First of all we define their dual role in promoting and inhibiting cancer progression depending on their phenotype. To continue, we describe the influence of different tumor microenvironment factors such as hypoxia on B cells functions and differentiation. Finally, the role of B cells in response to therapy and as potential target is examined. In accordance with the importance of B cells in immuno-oncology, we conclude that more studies are required to throw light on the precise role of B cells in the tumor microenvironment in order to have a better understanding of their functions, and to design new strategies that efficiently target these cells by immunotherapy.
Pediatric Compounding Pharmacy: Taking on the Responsibility of Providing Quality Customized Prescriptions
Authors: Heitman Tricia , Day A. J. , Bassani August S.
Published: May 2019

Compounding pharmacy has an important role to play in the field of pediatric medicine. These specialized pharmacies can offer solutions to the unique patient needs that arise in the pediatric population. Medication can be tailored to the child to allow better compliance in cases when the commercial product is unable to meet the needs of the patient. For example, a suspension, suppository, or lozenge formulation is sometimes needed when the manufactured products are only offered as solid oral dosage forms. Sensory processing disorder (SPD), patients with food allergies, and specific dietary needs can also be a big challenge for caregivers and practitioners who need alternatives to the commercially available forms. Three example cases are presented to help describe the process of collaboration between the pharmacist, patient, and doctor to solve the patient’s needs.
A Climatology of Atmospheric Patterns Associated with Red River Valley Blizzards
Authors: Kennedy Aaron , Trellinger Alexander , Grafenauer Thomas , Gust Gregory
Published: May 2019

Stretching along the border of North Dakota and Minnesota, The Red River Valley (RRV) of the North has the highest frequency of reported blizzards within the contiguous United States. Despite the numerous impacts these events have, few systematic studies exist that discuss the meteorological properties of blizzards. As a result, forecasting these events and lesser blowing snow events is an ongoing challenge. This study presents a climatology of atmospheric patterns associated with RRV blizzards for the winter seasons of 1979–1980 and 2017–2018. Patterns were identified using subjective and objective techniques using meteorological fields from the North American Regional Re-analysis (NARR). The RRV experiences, on average, 2.6 events per year. Blizzard frequency is bimodal, with peaks occurring in December and March. The events can largely be typed into four meteorological categories dependent on the forcing that drives the blizzard: Alberta Clippers, Arctic Fronts, Colorado Lows, and Hybrids. The objective classification of these blizzards using a competitive neural network known as the Self-Organizing Map (SOM) demonstrates that gross segregation of the events can be achieved with a small (eight-class) map. This implies that objective analysis techniques can be used to identify these events in weather and climate model output that may aid future forecasting and risk assessment projects.
Superhydrophobic Coatings from Recyclable Materials for Protection in a Real Sea Environment
Authors: Ferrari Michele , Benedetti Alessandro , Cirisano Francesca
Published: May 2019

Recyclable materials can be referred to as both those materials directly recycled from wastes and those derived from any kind of transformation before use. Highly water repellent coatings with wettability properties, known as superhydrophobic (SH), are related to surfaces with contact angles above 150° and a very small hysteresis. The small area available for these surfaces when in contact with water can be exploited in many applications in which interactions with an aqueous environment are usually desirable to be avoided, like for protection and friction reduction in a marine environment. SH coatings under investigation have been prepared starting from recyclable materials with the aim to provide a sustainable and low cost solution, with potential application to large surfaces in a marine environment. Wetting studies, surface characterization, and electrochemical tests show how these surfaces can be used in terms of fouling prevention and the protection of metals in underwater conditions.
Pre-Coated Fe–Ni Film to Promote Low-Pressure Carburizing of 14Cr14Co13Mo4 Steel
Authors: Yin Longcheng , Wang Tingjian , Ma Xinxin , Fu Zhongyuan , Hao Guodong , Li Liuhe , Wang Liqin
Published: May 2019

Case-hardening 14Cr14Co13Mo4 martensitic stainless steel needs to be carburized to improve surface performance. Low-pressure carburization has the benefit of having oxidation-free production and being ecofriendly. However, compared with the low-pressure carburization of the low-alloy steel, low-pressure carburization of the 14Cr14Co13Mo4 steel consumes more time and has a risk of network carbides. In order to promote carbon diffusion and avoid network carbide, Fe–Ni films with various thickness were electrodeposited on the 14Cr14Co13Mo4 steel prior to low-pressure carburization. The experimental results show that, under the same carburizing conditions, the surface carbon content decreases and the carburized layer increases with the increase of Fe–Ni film thickness. After the hardening heat treatment, the effective case depth (ECD) of the sample coated with 6.0 μm Fe–Ni film was increased by 29% compared to that of the uncoated sample. The morphology of carbides was a strip-shaped, discontinuous network distribution in the uncoated sample, while in the Fe–Ni coated samples, the carbides changed to a globular, uniformly dispersed distribution. The effect of Fe–Ni film on the low-pressure carburizing of steel is explained by the simulation of the carbon diffusion using DICTRA software. The Fe–Ni films reduce the steel surface carbon content in each boost stage of low-pressure carburizing and release carbon atoms in every diffusion stage. Through this adjustment mechanism, the steel surface carbon content can be reduced and carburized layer growth can be promoted.
Diffusion of Innovation: Case of Co-Design of Cabins in Mobile Work Machine Industry
Authors: Ellman Asko , Tiainen Tarja
Published: May 2019

This paper describes the development of using virtual reality for work content in one application area over a decade. Virtual reality technology has developed rapidly; from walk-in CAVE-like virtual environments to head-mounted displays within a decade. In this paper, the development is studied through the lens of diffusion of innovation theory, which focuses not only on innovation itself, but also on the social system. The development of virtual technology is studied by one case, which is cabin design in the mobile work machine industry. This design process has been especially suitable for using virtual reality technology.
Extending the pool of compatible peptide hydrogels for protein crystallization
Authors: Escolano-Casado Guillermo , Contreras-Montoya Rafael , Conejero-Muriel Mayte , Castellví Albert , Juanhuix Judith , Lopez-Lopez Modesto T. , Álvarez de Cienfuegos Luis , Gavira José A.
Published: May 2019

Short-peptide supramolecular (SPS) hydrogels are a class of materials that have been found to be useful for (bio)technological applications thanks to their biocompatible nature. Among the advantages reported for these peptides, their economic affordability and easy functionalization or modulation have turned them into excellent candidates for the development of functional biomaterials. We have recently demonstrated that SPS hydrogels can be used to produce high-quality protein crystals, improve their properties, or incorporate relevant materials within the crystals. In this work, we prove that hydrogels based on methionine and tyrosine are also good candidates for growing high-quality crystals of the three model proteins: lysozyme, glucose isomerase, and thaumatin.
Czochralski Growth, Magnetic Properties and Faraday Characteristics of CeAlO3 Crystals
Authors: Guo Feiyun , Li Qiyuan , Zhang Huaimin , Yang Xiongsheng , Tao Zhen , Chen Xin , Chen Jianzhong
Published: May 2019

CeAlO3 crystals were grown in different growth atmospheres by the Czochralski method. The lattice parameters and space group of CeAlO3 crystal were determined by Rietveld structure refinement of X-ray diffraction (XRD) data. The influence of Ce4+ ions in the crystal on the transmittance and crystal color was confirmed by XPS analysis. Magnetization curve at room temperature and temperature dependencies of the magnetic susceptibility in two different directions were measured, indicating that CeAlO3 crystal has remarkable magnetic anisotropy and there is an abnormal magnetic behavior in the vertical <001> direction in the temperature range of 50–150 K. Faraday characteristics of CeAlO3 crystal were investigated at room temperature. Verdet constants of CeAlO3 at 532, 635 and 1064 nm are about 2.1 times as large as those of CeF3. The reason of large Verdet constants was analyzed based on the Van Vleck–Hebb theory and the magnetic circular dichroism (MCD) spectrum.
Structural Chemistry of Akdalaite, Al10O14(OH)2, the Isostructural Aluminum Analogue of Ferrihydrite
Authors: Parise John B. , Xia Bingying , Simonson Jack W. , Woerner William R. , Plonka Anna M. , Phillips Brian L. , Ehm Lars
Published: May 2019

As part of an effort to characterize clusters and intermediate phases likely to be encountered along solution reaction pathways that produce iron and aluminum oxide-hydroxides from Fe and Al precursors, the complete structure of Al10O14(OH)2 (akdalaite) was determined from a combination of single-crystal X-ray diffraction (SC-XRD) data collected at 100 K to define the Al and O positions, and solid-state nuclear magnetic resonance (NMR) and neutron powder diffraction (NPD) data collected at room temperature (~300 K) to precisely determine the nature of hydrogen in the structure. Two different synthesis routes produced different crystal morphologies. Using an aluminum oxyhydroxide floc made from mixing AlCl3 and 0.48 M NaOH, the product had uniform needle morphology, while using nanocrystalline boehmite (Vista Chemical Company Catapal D alumina) as the starting material produced hexagonal plates. Akdalaite crystallizes in the space group P63mc with lattice parameters of a = 5.6244(3) Å and c = 8.8417(3) Å (SC-XRD) and a = 5.57610(2) Å and c = 8.77247(6) Å (NPD). The crystal structure features Al13O40 Keggin clusters. The structural chemistry of akdalaite is nonideal but broadly conforms to that of ferrihydrite, the nanomineral with which it is isostructural.
Insight into the Optoelectronic and Thermoelectric Properties of Mn Doped ZnTe from First Principles Calculation
Authors: Khan Wilayat , Azam Sikander , Ullah Inam , Rani Malika , Younus Ayesha , Irfan Muhammad , Czaja Paweł , Kityk Iwan V.
Published: May 2019

Using DFT band structure simulations together with semi-classical Boltzmann transport kinetics equations, we have explored the optoelectronic and transport features of MnxZn1−xTe (x = 8% and 16%) crystals. Optimization of the doping and related technological processes it is extremely important for optimization of the technological parameters. The Generalized Gradient Approximation is applied to compute the corresponding band structure parameters. We have applied the Generalized Gradient Approximation Plus U (GGA+U). We have demonstrated that MnxZn1−xTe (x = 8% and 16%) is a direct type band semiconductor with principal energy gap values equal to 2.20 and 2.0 eV for x = 8% and 16%, respectively. The energy gap demonstrates significant decrease with increasing Mn content. Additionally, the origin of the corresponding bands is explored from the electronic density of states. The optical dispersion functions are calculated from the spectra of dielectric function. The theoretical simulations performed unambiguously showed that the titled materials are simultaneously promising optoelectronic and thermoelectric devices. The theoretical simulations performed showed ways for amendment of their transport properties by replacement of particular ions.
Point of Sale (POS) Data from a Supermarket: Transactions and Cashier Operations
Authors: Antczak Tomasz , Weron Rafał
Published: May 2019

As queues in supermarkets seem to be inevitable, researchers try to find solutions that can improve and speed up the checkout process. This, however, requires access to real-world data for developing and validating models. With this objective in mind, we have prepared and made publicly available high-frequency datasets containing nearly six weeks of actual transactions and cashier operations from a grocery supermarket belonging to one of the major European retail chains. This dataset can provide insights on how the intensity and duration of checkout operations changes throughout the day and week.
Giant Hepatic Artery Aneurysm
Authors: Gossili Farid , Zacho Helle D.
Published: May 2019

Hepatic artery aneurysm (HAA) is the second most common type of visceral aneurysm. Giant HAAs (larger than 5 cm) are very rare. We present a case of an asymptomatic giant hepatic artery aneurysm (diameter 10.7 cm) discovered as an incidental finding on an 18F-fludeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) scan of a patient admitted for pretreatment staging of urothelial carcinoma.
Where Was Past Low-Entropy?
Authors: Rovelli Carlo
Published: May 2019

Where was the past low-entropy of the early universe located? Contrary to some popular answers, I argue that that the dominant source of low-entropy is the fact that a single degree of freedom, the scale factor, was not at equilibrium. I also discuss possible interpretations of the “improbability” of this early low-entropy.
Time-Frequency Analysis of Cardiovascular and Cardiorespiratory Interactions During Orthostatic Stress by Extended Partial Directed Coherence
Authors: Charleston-Villalobos Sonia , Reulecke Sina , Voss Andreas , Azimi-Sadjadi Mahmood R. , González-Camarena Ramón , Gaitán-González Mercedes J. , González-Hermosillo Jesús A. , Hernández-Pacheco Guadalupe , Schulz Steffen , Aljama-Corrales Tomás
Published: May 2019

In this study, the linear method of extended partial directed coherence (ePDC) was applied to establish the temporal dynamic behavior of cardiovascular and cardiorespiratory interactions during orthostatic stress at a 70° head-up tilt (HUT) test on young age-matched healthy subjects and patients with orthostatic intolerance (OI), both male and female. Twenty 5-min windows were used to analyze the minute-wise progression of interactions from 5 min in a supine position (baseline, BL) until 18 min of the orthostatic phase (OP) without including pre-syncopal phases. Gender differences in controls were present in cardiorespiratory interactions during OP without compromised autonomic regulation. However in patients, analysis by ePDC revealed considerable dynamic alterations within cardiovascular and cardiorespiratory interactions over the temporal course during the HUT test. Considering the young female patients with OI, the information flow from heart rate to systolic blood pressure (mechanical modulation) was already increased before the tilt-up, the information flow from systolic blood pressure to heart rate (neural baroreflex) increased during OP, while the information flow from respiration to heart rate (respiratory sinus arrhythmia) decreased during the complete HUT test. Findings revealed impaired cardiovascular interactions in patients with orthostatic intolerance and confirmed the usefulness of ePDC for causality analysis.
Support Vector Machine-Based Transmit Antenna Allocation for Multiuser Communication Systems
Authors: Lin Huifa , Shin Won-Yong , Joung Jingon
Published: May 2019

In this paper, a support vector machine (SVM) technique has been applied to an antenna allocation system with multiple antennas in multiuser downlink communications. Here, only the channel magnitude information is available at the transmitter. Thus, a subset of transmit antennas that can reduce multiuser interference is selected based on such partial channel state information to support multiple users. For training, we generate the feature vectors by fully utilizing the characteristics of the interference-limited setup in the multiuser downlink system and determine the corresponding class label by evaluating a key performance indicator, i.e., sum rate in multiuser communications. Using test channels, we evaluate the performance of our antenna allocation system invoking the SVM-based allocation and optimization-based allocation, in terms of sum-rate performance and computational complexity. Rigorous testing allowed for a comparison of a SVM algorithm design between one-vs-one (OVO) and one-vs-all (OVA) strategies and a kernel function: (i) OVA is preferable to OVO since OVA can achieve almost the same sum rate as OVO with significantly reduced computational complexity, (ii) a Gaussian function is a good choice as the kernel function for the SVM, and (iii) the variance (kernel scale) and penalty parameter (box constraint) of an SVM kernel function are determined by 21.56 and 7.67, respectively. Further simulation results revealed that the designed SVM-based approach can remarkably reduce the time complexity compared to a traditional optimization-based approach, at the cost of marginal sum rate degradation. Our proposed framework offers some important insights for intelligently combining machine learning techniques and multiuser wireless communications.
Heat Transfer and Entropy Generation Abilities of MWCNTs/GNPs Hybrid Nanofluids in Microtubes
Authors: Hussien Ahmed A. , Abdullah Mohd Z. , Yusop Nadiahnor Md , Al-Kouz Wael , Mahmoudi Ebrahim , Mehrali Mohammad
Published: May 2019

Massive improvements in the thermophysical properties of nanofluids over conventional fluids have led to the rapid evolution of using multiwalled carbon nanotubes (MWCNTs) and graphene nanoplatelets (GNPs) in the field of heat transfer. In this study, the heat transfer and entropy generation abilities of MWCNTs/GNPs hybrid nanofluids were explored. Experiments on forced convective flow through a brass microtube with 300 µm inner diameter and 0.27 m in length were performed under uniform heat flux. MWCNTs/GNPs hybrid nanofluids were developed by adding 0.035 wt.% GNPs to MWCNTs water-based nanofluids with mass fractions of 0.075–0.125 wt.%. The range of the Reynolds number in this experiment was maintained at Re = 200–500. Results showed that the conventional approach for predicting the heat transfer coefficient was applicable for microtubes. The heat transfer coefficient increased markedly with the use of MWCNTs and MWCNTs/GNPs nanofluids, with increased pressure dropping by 12.4%. Results further showed a reduction by 37.5% in the total entropy generation rate in microtubes for hybrid nanofluids. Overall, MWCNTs/GNPs hybrid nanofluids can be used as alternative fluids in cooling systems for thermal applications.