bluuebunny/crossref_metadata_2025 huggingface dataset:
The Experience of Implementing a National Antimicrobial Resistance Surveillance System in Brazil
Authors: Pillonetto Marcelo , Jordão Regiane Tigulini de Souza , Andraus Gabriel Savogin , Bergamo Ricardo , Rocha Fabiano Barreto , Onishi Mayara Caroline , Almeida Bernardo Montesanti Machado de , Nogueira Keite da Silva , Dal Lin Amanda , Dias Viviane Maria de Carvalho Hessel , Abreu André Luiz de
Published: January 2021

Antimicrobial resistance (AMR) is a major public health threat of global proportions, which has the potential to lead to approximately ten million deaths per year by 2050. Pressured by this wicked problem, in 2014, the World Health Organization launched a call for member states to share AMR data through the implementation of the Global Antimicrobial Resistance Surveillance System (GLASS), to appropriately scale and monitor the general situation world-widely. In 2017, Brazil joined GLASS and, in 2018, started its own national antimicrobial surveillance program (BR-GLASS) to understand the impact of resistance in the country. We compiled data obtained from the complete routine of three hospitals' microbiology labs during the year of 2018. This pilot data sums up to 200,874 antimicrobial susceptibility test results from 11,347 isolates. It represents 119 different microorganisms recovered from 44 distinct types of clinical samples. Specimens came from patients originating from 301 Brazilian cities, with 4,950 of these isolates from presumed Healthcare-Associated Infections (HAIs) and the other 6,397 community-acquired cases. The female population offered 58% of the collected samples, while the other 42% were of male origin. The urinary tract was the most common topography (6,372/11,347 isolates), followed by blood samples (2,072/11,347). Gram-negative predominated the bacterial isolates: Escherichia coli was the most prevalent in general, representing 4,030 isolates (89.0% of these from the urinary tract). Coagulase-negative Staphylococci were the most prevalent bacteria in blood samples. Besides these two species, the ESKAPE group have consolidated their prevalence. Regarding drug susceptibility results, 141,648 (70.5%) were susceptible, 9,950 (4.9%) intermediate, and 49,276 (24.5%) resistant. Acinetobacter baumannii was the most worrisome microorganism, with 65.3% of the overall antimicrobial susceptibility tests showing resistance, followed by ESBL-producing Klebsiella pneumoniae, with a global resistance rate of 59%. Although this is a pilot project (still limited to one state), this database shows the importance of a nation-wide surveillance program,[153mm][-12mm] Q14 especially considering it already had patients coming from 301 distinct counties and 18 different states. The BR-GLASS Program is an ongoing project that intends to encompass at least 95 hospitals distributed in all five geographical regions in Brazil within the next 5 years.
Factors Associated With Low Physical Activity in Two Latin American Populations at Risk of Developing Type 2 Diabetes: An Exploratory Analysis
Authors: Acosta Tania , Tuesca Rafael , Florez Karen , Barengo Noël C. , Anillo Luis , Flórez-García Victor , Acosta Jorge , Carvajal Liliana , de la Rosa Sandra , Pachón María Julieta , Aschner Pablo
Published: January 2021

Low level of physical activity is a risk factor for chronic non-communicable diseases. Specifically, people at risk of Type 2 Diabetes (T2D) have shown to benefit from being physically active. The objective of this study was to explore what factors were associated with low physical activity in people at high risk of T2D living in Bogota and Barranquilla, Colombia.

Methodology: Cross-sectional study using baseline data from a quasi-experimental clinical trial (PREDICOL Project). The study included 1,135 participants of Bogota and Barranquilla that presented a high risk of developing T2D according to the Finnish Diabetes Risk Score (>12 points) and who underwent an oral glucose tolerance test. The main outcome variable was the level of physical activity assessed by the International Physical Activity Questionnaire. Unadjusted and adjusted logistic regression analysis were used to calculate odds ratios (OR) and the corresponding 95% confidence intervals (CI).

Results: In total, 72.5% of the study participants had low level of physical activity. Participants in the age group between 45 and 54 years showed 74% greater odds of having low physical activity compared with the youngest age group (OR 1.74, 95% CI 1.1 −2.8). People living in Barranquilla were eight times more likely to have low physical activity compared with those in Bogotá D.C. (OR 8.1, 95% CI 5.7 to 11.4).

Conclusion: A large proportion of the population at risk of developing D2T in two large cities of Colombia have a sedentary lifestyle. Interventions should be designed and implemented in order to increase physical activity in these populations.
What Are the Determinants of a Workplace Health Promotion? Application of a Social Marketing Model in Identifying Determinants of Physical Activity in the Workplace (a Qualitative Study)
Authors: Kaveh Mohammad Hossein , Layeghiasl Mehdi , Nazari Mahin , Ghahremani Leila , Karimi Masoud
Published: January 2021

Background and objective:Physical activity is an important behavior to reduce the risk of non-communicable diseases. Providing the context for physical activity in the workplace in addition to promoting the employees' physical and mental health has significant economic benefits for organizations. We conducted the present study using a social marketing model to explain the determinants of a Workplace Health Promotion in promoting physical activity among employees of government organizations in Yasuj, Iran.

Materials and methods:The present study was qualitative research with directed content analysis based on the social marketing model. Thirty-three employees of government organizations in Yasuj were included in the study using the purposive sampling method. The data collection method included semi-structured interviews and observation. Data analysis was performed manually and by the qualitative content analysis method. The implementation data were systematically sorted and analyzed and classified into five steps.

Results:Organizational structure, organizational policies, and a supportive interpersonal climate were extracted and categorized as characteristics of workplace health promotion programs from participant interviews. In addition to participants' emphasis on receiving information from reputable sources, virtual communication networks such as WhatsApp and real communication networks such as physicians and specialists were their preferred media for education and information.

Conclusion:Due to the complexity of workplace, diversity, and multiplicity of factors and determinants of physical activity, the findings of the present study will be a basis for designing an appropriate and effective intervention and organizational changes to promote physical activities among employees in the future.
Acceptability of Self-Sampling for Cervical Cancer Screening Among Women Living With HIV and HIV-Negative Women in Limbé, Cameroon
Authors: Pierz Amanda J. , Ajeh Rogers , Fuhngwa Norbert , Nasah Judith , Dzudie Anastase , Nkeng Relindis , Anastos Kathryn M. , Castle Philip E. , Adedimeji Adebola
Published: January 2021

Introduction: Like many countries in Sub-Saharan Africa, Cameroon has a high burden of cervical cancer and low availability and uptake of screening. Self-collection has the potential to increase the uptake of cervical cancer screening among Cameroon women. This paper explores patient and community insights surrounding self-collection among women living with HIV and HIV[-] women as well as the barriers and facilitators to obtaining and utilizing self-collected specimens in cervical cancer screening programs.

Materials and methods: We utilized an exploratory qualitative approach to obtain data through focus group discussions and in-depth interviews during data collection that took place from May to August 2018. A two-stage sampling strategy was used to select 80 women who participated in six focus group discussions and eight in-depth interviews. We utilized the socio-ecological framework to guide data analysis.

Results: All participants indicated that self-sampling was an acceptable method of specimen collection and should be offered as an option for cervical cancer screening in Cameroon. Whereas, most women, regardless of HIV status, preferred the option for self-collection, barriers were identified, such as lack of education about self-collection procedure, being uncomfortable, embarrassed or in pain from the procedure, fear of consequences, perceived competence about ability to self-collect and privacy and confidentiality. We also found that HIV-related stigma was a major concern for HIV[-] women that could prevent them from accessing cervical cancer screening integrated within HIV treatment settings.

Conclusions: To promote self-collection for cervical cancer screening, educational interventions with both patients and providers are necessary to increase knowledge of and overall willingness to utilize self-collection. Further research is recommended to examine the role of stigma for HIV[-] women in screening locations associated with HIV treatment.
Adoption Intensity of Selected Organic-Based Soil Fertility Management Technologies in the Central Highlands of Kenya
Authors: Mwaura George G. , Kiboi Milka N. , Bett Eric K. , Mugwe Jayne N. , Muriuki Anne , Nicolay Gian , Ngetich Felix K.
Published: January 2021

Soil fertility decline continues to be a major challenge limiting agricultural productivity globally. Despite the novelty of organic-based technologies in enhancing agricultural production in Kenya's central highlands, adoption is low. Therefore, we carried out a cross-sectional household survey of 300 randomly selected smallholder farmers to determine the specific organic-based practices by farmers; and the socioeconomic factors that influence the adoption intensity of selected organic-based technologies. We used descriptive statistics to summarize the data and the Tobit regression model to evaluate the socioeconomic determinants of adoption intensity of selected organic-based technologies. We identified nine organic-based technologies that had different adoption rates among the farmers. The majority of the farmers had adopted manure (97%) and manure combined with fertilizer (92%) in Murang'a and Tharaka-Nithi, respectively. Manure was applied to the largest land in Murang'a with 31% of the cultivated land. In comparison, manure combined with fertilizer had the highest adoption intensity in Tharaka-Nithi applied to about 25% of the cultivated land. Gender, age of the household head, level of education, household size, access to external labor, training, Tropical Livestock Unit, agriculture group membership, access to credit, land cultivated, and farming experience influenced the adoption intensity of organic-based technologies among smallholder farmers. Based on the smallholder farmers' adoption behavior, this study can be used to disaggregate the farming households better in order to tailor specific organic-based soil fertility technologies solutions that meet their unique needs. One group would be those households that face specific constraints, as reflected in their low adoption rates, women-headed households and older farmers, and thus require more targeted / intensive efforts to overcome these barriers. The other group would be those households that require less focus because, when confronted with the technologies, they are more likely to adopt them easily, for example, the male-headed households. Hence, the smallholder farmers' adoption behavior, can enable policymakers to form a base for designing appropriate policies that encourage the adoption of organic-based soil fertility technology by smallholder farmers.
Clinical Characteristics and Treatment Strategy of Retroperitoneal Schwannoma Adjacent to Important Abdominal Vessels: Three Case Reports and Literature Review
Authors: Wu Qi , Liu Bingqiang , Lu Jun , Chang Hong
Published: January 2021

Purpose: The purpose of this study was to review the clinical characteristics and treatment strategies of patients with retroperitoneal schwannomas adjacent to important abdominal vessels.

Case Presentation: A total of three patients with retroperitoneal schwannoma immediately adjacent to important blood vessels in the abdominal cavity underwent successful surgical resection. They all had symptoms of abdominal pain and discomfort, two cases underwent three-dimensional reconstruction. There were no serious complications such as peripheral blood vessels and organ damage in all three cases. One case had chyle leakage after surgery, conservative treatment was successfully discharged.

Conclusions: Retroperitoneal schwannomas immediately adjacent to important abdominal vessels have unique clinical characteristics. Preoperative three-dimensional reconstruction can fully show the local vascular relationship of the tumor, which is conducive to surgical planning and risk assessment. Benign tumors with large size and adjacent complex vessels can still be completely resected by surgery. Laparotomy resection is safe and feasible.
Bladder and Bowel Management in Dogs With Spinal Cord Injury
Authors: Granger Nicolas , Olby Natasha J. , Nout-Lomas Yvette S. ,   The Canine Spinal Cord Injury Consortium (CANSORT-SCI)
Published: January 2021

Spinal cord injury in companion dogs can lead to urinary and fecal incontinence or retention, depending on the severity, and localization of the lesion along the canine nervous system. The bladder and gastrointestinal dysfunction caused by lesions of the autonomic system can be difficult to recognize, interpret and are easily overlooked. Nevertheless, it is crucial to maintain a high degree of awareness of the impact of micturition and defecation disturbances on the animal's condition, welfare and on the owner. The management of these disabilities is all the more challenging that the autonomic nervous system physiology is a complex topic. In this review, we propose to briefly remind the reader the physiology of micturition and defecation in dogs. We then present the bladder and gastrointestinal clinical signs associated with sacral lesions (i.e., the L7-S3 spinal cord segments and nerves) and supra-sacral lesions (i.e., cranial to the L7 spinal cord segment), largely in the context of intervertebral disc herniation. We summarize what is known about the natural recovery of urinary and fecal continence in dogs after spinal cord injury. In particular we review the incidence of urinary tract infection after injury. We finally explore the past and recent literature describing management of urinary and fecal dysfunction in the acute and chronic phase of spinal cord injury. This comprises medical therapies but importantly a number of surgical options, some known for decades such as sacral nerve stimulation, that might spark some interest in the field of spinal cord injury in companion dogs.
The Epidemiological and Economic Impact of a Potential Foot-and-Mouth Disease Outbreak in Austria
Authors: Marschik Tatiana , Kopacka Ian , Stockreiter Simon , Schmoll Friedrich , Hiesel Jörg , Höflechner-Pöltl Andrea , Käsbohrer Annemarie , Pinior Beate
Published: January 2021

An outbreak of foot-and mouth disease (FMD) in an FMD-free country such as Austria would likely have serious consequences for the national livestock sector and economy. The objective of this study was to analyse the epidemiological and economic impact of an FMD outbreak in Austria in order to (i) evaluate the effectiveness of different control measures in two Austrian regions with different livestock structure and density, (ii) analyse the associated costs of the control measures and the losses resulting from trade restrictions on livestock and livestock products and (iii) assess the resources that would be required to control the FMD outbreak. The European Foot-and-Mouth Disease Spread Model (EuFMDiS) was used to simulate a potential FMD outbreak. Based on the epidemiological outputs of the model, the economic impact of the outbreak was assessed. The analysis of the simulations showed that the success of control strategies depends largely on the type of control measures, the geographical location, the availability of sufficient resources, and the speed of intervention. The comparison of different control strategies suggested that from an economic point of view the implementation of additional control measures, such as pre-emptive depopulation of susceptible herds, would be efficient if the epidemic started in an area with high livestock density. Depending on the chosen control measures and the affected region, the majority of the total costs would be attributable to export losses (e.g., each day of an FMD epidemic costs Austria € 9–16 million). Our analysis indicated that the currently estimated resources for surveillance, cleaning, and disinfection during an FMD outbreak in Austria would be insufficient, which would lead to an extended epidemic control duration. We have shown that the control of an FMD outbreak can be improved by implementing a contingency strategy adapted to the affected region and by placing particular focus on an optimal resource allocation and rapid detection of the disease in Austria. The model results can assist veterinary authorities in planning resources and implementing cost-effective control measures for future outbreaks of highly contagious viral diseases.
Comparison of Canine and Feline Adipose-Derived Mesenchymal Stem Cells/Medicinal Signaling Cells With Regard to Cell Surface Marker Expression, Viability, Proliferation, and Differentiation Potential
Authors: Voga Metka , Kovač Valerija , Majdic Gregor
Published: January 2021

Remarkable immunomodulatory abilities of mesenchymal stem cells, also called multipotent mesenchymal stromal cells or medicinal signaling cells (MSCs), have entailed significant advances in veterinary regenerative medicine in recent years. Despite positive outcomes from MSC therapies in various diseases in dogs and cats, differences in MSC characteristics between small animal veterinary patients are not well-known. We performed a comparative study of cells' surface marker expression, viability, proliferation, and differentiation capacity of adipose-derived MSCs (ADMSCs) from dogs and domestic cats. The same growth media and methods were used to isolate, characterize, and culture canine and feline ADMSCs. Adipose tissue was collected from 11 dogs and 8 cats of both sexes. The expression of surface markers CD44, CD90, and CD34 was detected by flow cytometry. Viability at passage 3 was measured with the hemocytometer and compared to the viability measured by flow cytometry after 1 day of handling. The proliferation potential of MSCs was measured by calculating cell doubling and cell doubling time from second to eighth passage. Differentiation potential was determined at early and late passages by inducing cells toward adipogenic, osteogenic, and chondrogenic differentiation using commercial media. Our study shows that the percentage of CD44+CD90+ and CD34−/− cells is higher in cells from dogs than in cells from cats. The viability of cells measured by two different methods at passage 3 differed between the species, and finally, canine ADMSCs possess greater proliferation and differentiation potential in comparison to the feline ADMSCs.
Unmanned Aerial Vehicle Pitch Control Using Deep Reinforcement Learning with Discrete Actions in Wind Tunnel Test
Authors: Wada Daichi , Araujo-Estrada Sergio A. , Windsor Shane
Published: January 2021

Deep reinforcement learning is a promising method for training a nonlinear attitude controller for fixed-wing unmanned aerial vehicles. Until now, proof-of-concept studies have demonstrated successful attitude control in simulation. However, detailed experimental investigations have not yet been conducted. This study applied deep reinforcement learning for one-degree-of-freedom pitch control in wind tunnel tests with the aim of gaining practical understandings of attitude control application. Three controllers with different discrete action choices, that is, elevator angles, were designed. The controllers with larger action rates exhibited better performance in terms of following angle-of-attack commands. The root mean square errors for tracking angle-of-attack commands decreased from 3.42° to 1.99° as the maximum action rate increased from 10°/s to 50°/s. The comparison between experimental and simulation results showed that the controller with a smaller action rate experienced the friction effect, and the controllers with larger action rates experienced fluctuating behaviors in elevator maneuvers owing to delay. The investigation of the effect of friction and delay on pitch control highlighted the importance of conducting experiments to understand actual control performances, specifically when the controllers were trained with a low-fidelity model.
Drought Tolerant near Isogenic Lines (NILs) of Pusa 44 Developed through Marker Assisted Introgression of qDTY2.1 and qDTY3.1 Enhances Yield under Reproductive Stage Drought Stress
Authors: Dwivedi Priyanka , Ramawat Naleeni , Dhawan Gaurav , Gopala Krishnan Subbaiyan , Vinod Kunnummal Kurungara , Singh Madan Pal , Nagarajan Mariappan , Bhowmick Prolay Kumar , Mandal Nimai Prasad , Perraju Puvvada , Bollinedi Haritha , Ellur Ranjith Kumar , Singh Ashok Kumar
Published: January 2021

Reproductive stage drought stress (RSDS) is detrimental for rice, which affects its productivity as well as grain quality. In the present study, we introgressed two major quantitative trait loci (QTLs), namely, qDTY2.1 and qDTY3.1, governing RSDS tolerance in a popular high yielding non-aromatic rice cultivar, Pusa 44, through marker-assisted backcross breeding (MABB). Pusa 44 is highly sensitive to RSDS, which restricts its cultivation across drought-prone environments. Foreground selection was carried out using markers, RM520 for qDTY3.1 and RM 521 for qDTY2.1. Background selection was achieved with 97 polymorphic SSR markers in tandem with phenotypic selection to achieve faster recurrent parent genome (RPG) recovery. Three successive backcrosses followed by three selfings aided RPG recoveries of 98.6% to 99.4% among 31 near isogenic lines (NILs). Fourteen NILs were found to be significantly superior in yield and grain quality under RSDS with higher drought tolerance efficiency (DTE) than Pusa 44. Among these, the evaluation of two promising NILs in the multilocational trial during Kharif 2019 showed that they were significantly superior to Pusa 44 under reproductive stage drought stress, while performing on par with Pusa 44 under normal irrigated conditions. These di-QTL pyramided drought-tolerant NILs are in the final stages of testing the All India Coordinated Rice Improvement Project varietal trials for cultivar release. Alternately, the elite drought-tolerant Pusa 44 NILs will serve as an invaluable source of drought tolerance in rice improvement.
Phenotypic Evaluation of Fire Blight Outbreak in the USDA Malus Collection
Authors: Dougherty Laura , Wallis Anna , Cox Kerik , Zhong Gan-Yuan , Gutierrez Benjamin
Published: January 2021

Fire blight, caused by pathogen Erwinia amylovora, is a major disease in Malus. Biological, chemical and cultural controls are efficient to manage fire blight, while rootstocks, and host resistance can limit damages. During the 2020 season a naturally occurring fire blight outbreak occurred in the United States Department of Agriculture (USDA) Malus collection, providing a unique opportunity to evaluate the diverse collection for fire blight susceptibility. The E. amylovora strain in the collection was identified as streptomycin resistant and characterized as CRISPR (clustered regularly interspaced short palindromic repeats) spacer array profile, 41:23:38. Fire blight severity was assessed using two approaches: (1) Average severity percentage, where the number of infected shoots was divided by the total number of shoots for the east and west facing sides of the tree; and (2) cut severity rating, where the trees were visually assessed after fire blight removal for amount of tree removed. Overall, 1142 trees of 41 Malus species were assessed for average severity and 2525 trees of 48 species were assessed for cut severity. A subset of 667 trees were for average severity in June and July to understand the disease progression. The species and trees presented here, can provide insight for future genetic fire blight resistance studies.
Two Hours of Separation Prior to Milking: Is This Strategy Stressful for Jennies and Their Foals?
Authors: de Souza Farias Sharacely , Montechese Ana Carolina Dierings , Bernardino Thiago , Rodrigues Paulo Henrique Mazza , de Araujo Oliveira Chiara Albano , Zanella Adroaldo José
Published: January 2021

The goal of this study was to assess whether or not a separation period of 2 h is stressful for jennies and foals, as measured by changes in behaviour, salivary cortisol, and milk production. This study was reviewed and approved by the Committee for the Use and Care of Animals in Research (CEUA) of the School of Veterinary Medicine and Animal Science of the University of São Paulo. Fourteen multiparous Pêga jennies (245 kg average body weight) and their foals were assessed from day 45 to 135 of lactation. Dams and foals were separated for 2 h prior to milking. Behavioural assessments and saliva samples were collected before and after separation, every 15 days, resulting in 14 samples per individual animal. Behavioural states (affiliative and inactivity) and events (agonistic, abnormal, eliminative and vocalisations) of the jennies were observed during 6 min in both periods. Moreover, milk yield was measured. Few significant behavioural and salivary cortisol changes were observed, and milk yield was not affected by cortisol levels in response to the separation. The 2-h separation period, on the basis of the collected variables, did not appear to be stressful for the assessed group of Pêga jennies or foals; however, their ability to adapt to milking routine stress remains to be investigated.
Defining the Scope of Antimicrobial Stewardship Interventions on the Prescription Quality of Antibiotics for Surgical Intra-Abdominal Infections
Authors: Surat Güzin , Vogel Ulrich , Wiegering Armin , Germer Christoph-Thomas , Lock Johan Friso
Published: January 2021

Background: The aim of this study was to assess the impact of antimicrobial stewardship interventions on surgical antibiotic prescription behavior in the management of non-elective surgical intra-abdominal infections, focusing on postoperative antibiotic use, including the appropriateness of indications. Methods: A single-center quality improvement study with retrospective evaluation of the impact of antimicrobial stewardship measures on optimizing antibacterial use in intra-abdominal infections requiring emergency surgery was performed. The study was conducted in a tertiary hospital in Germany from January 1, 2016, to January 30, 2020, three years after putting a set of antimicrobial stewardship standards into effect. Results: 767 patients were analyzed (n = 495 in 2016 and 2017, the baseline period; n = 272 in 2018, the antimicrobial stewardship period). The total days of therapy per 100 patient days declined from 47.0 to 42.2 days (p = 0.035). The rate of patients receiving postoperative therapy decreased from 56.8% to 45.2% (p = 0.002), comparing both periods. There was a significant decline in the rate of inappropriate indications (17.4% to 8.1 %, p = 0.015) as well as a significant change from broad-spectrum to narrow-spectrum antibiotic use (28.8% to 6.5%, p ≤ 0.001) for postoperative therapy. The significant decline in antibiotic use did not affect either clinical outcomes or the rate of postoperative wound complications. Conclusions: Postoperative antibiotic use for intra-abdominal infections could be significantly reduced by antimicrobial stewardship interventions. The identification of inappropriate indications remains a key target for antimicrobial stewardship programs.
Antimicrobial Resistance of Non-O157 Shiga Toxin-Producing Escherichia coli Isolated from Humans and Domestic Animals
Authors: Pan Yanyu , Hu Bin , Bai Xiangning , Yang Xi , Cao Lijiao , Liu Qian , Sun Hui , Li Juan , Zhang Ji , Jin Dong , Xiong Yanwen
Published: January 2021

Non-O157 Shiga toxin-producing Escherichia coli (STEC) is an important pathogen that can cause zoonotic diseases. To investigate the antimicrobial resistance of STEC in China, non-O157 STEC isolates, recovered from domestic animals and humans from 12 provinces, were analyzed using antimicrobial susceptibility testing and whole genome characterization. Out of the 298 isolates tested, 115 strains showed resistance to at least one antimicrobial and 85 strains showed multidrug resistance. The highest resistance rate was to tetracycline (32.6%), followed by nalidixic acid (25.2%) and chloramphenicol and azithromycin (both 18.8%). However, imipenem and meropenem were effective against all isolates. Antimicrobial resistance patterns varied among strains from different sources. Strains from pig, sheep, humans, and cattle showed resistance rates of 100.0%, 46.9%, 30.3%, and 6.3% to one or more antimicrobials, respectively. Forty-three genes related to 11 antimicrobial classes were identified among these strains. The colistin-resistance gene mcr was only carried by strains from pigs. A new fosfomycin-resistant gene, fosA7, was detected in strains from humans, cattle, and sheep. Whole genome phylogenetic analysis showed that strains from the four sources were genetically diverse and scattered throughout the phylogenetic tree; however, some strains from the same source had a tendency to cluster closely. These results provide a reference to monitor the emergence and spread of multidrug resistant STEC strains among animals and humans. Furthermore, with a better understanding of antimicrobial genotypes and phenotypes among the diverse STEC strains obtained, this study could guide the administration of antimicrobial drugs in STEC infections when necessary.
Characterization of TGF-β by Induced Oxidative Stress in Human Trabecular Meshwork Cells
Authors: Chen Hsin-Yi , Chou Hsiu-Chuan , Ho Yi-Jung , Chang Shing-Jyh , Liao En-Chi , Wei Yu-Shan , Lin Meng-Wei , Wang Yi-Shiuan , Chien Yu-An , Yu Xin-Ru , Kung Hsiang-Yu , Yang Chu-Chun , Chen Jia-Yu , Chan Hong-Lin , Ko Mei-Lan
Published: January 2021

Oxidative stress generated by reactive oxygen species (ROS) plays a critical role in the pathomechanism of glaucoma, which is a multifactorial blinding disease that may cause irreversible damage within human trabecular meshwork cells (HTMCs). It is known that the transforming growth factor-β (TGF-β) signaling pathway is an important component of oxidative stress-induced damage related to extracellular matrix (ECM) fibrosis and activates cell antioxidative mechanisms. To elucidate the dual potential roles and regulatory mechanisms of TGF-β in effects on HTMCs, we established an in vitro oxidative model using hydrogen peroxide (H2O2) and further focused on TGF-β-related oxidative stress pathways and the related signal transduction. Via a series of cell functional qualitative analyses to detect related protein level alterations and cell fibrosis status, we illustrated the role of TGF-β1 and TGF-β2 in oxidative stress-induced injury by shTGF-β1 and shTGF-β2 knockdown or added recombinant human TGF-β1 protein (rhTGF-β1). The results of protein level showed that p38 MAPK, TGF-β, and its related SMAD family were activated after H2O2 stimulation. Cell functional assays showed that HTMCs with H2O2 exposure duration had a more irregular actin architecture compared to normal TM cells. Data with rhTGF-β1 (1 ng/mL) pretreatment reduced the cell apoptosis rate and amount of reactive oxygen species (ROS), while it also enhanced survival. Furthermore, TGF-β1 and TGF-β2 in terms of antioxidant signaling were related to the activation of collagen I and laminin, which are fibrosis-response proteins. Succinctly, our study demonstrated that low concentrations of TGF-β1 (1 ng/mL) preserves HTMCs from free radical-mediated injury by p-p38 MAPK level and p-AKT signaling balance, presenting a signaling transduction mechanism of TGF-β1 in HTMC oxidative stress-related therapies.
Nitric Oxide and Hydrogen Sulfide Coordinately Reduce Glucose Sensitivity and Decrease Oxidative Stress via Ascorbate-Glutathione Cycle in Heat-Stressed Wheat (Triticum aestivum L.) Plants
Authors: Iqbal Noushina , Umar Shahid , Khan Nafees A. , Corpas Francisco J.
Published: January 2021

The involvement of nitric oxide (NO) and hydrogen sulfide (H2S) in countermanding heat-inhibited photosynthetic features were studied in wheat (Triticum aestivum L.). Heat stress (HS) was employed at 40 °C after establishment for 6 h daily, and then plants were allowed to recover at 25 °C and grown for 30 days. Glucose (Glc) content increased under HS and repressed plant photosynthetic ability, but the application of sodium nitroprusside (SNP, as NO donor) either alone or with sodium hydrosulfide (NaHS, as H2S donor) reduced Glc-mediated photosynthetic suppression by enhancing ascorbate-glutathione (AsA-GSH) metabolism and antioxidant system, which reduced oxidative stress with decreased H2O2 and TBARS content. Oxidative stress reduction or inhibiting Glc repression was maximum with combined SNP and NaHS treatment, which was substantiated by 2-4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) and hypotaurine (HT), scavengers for NO and H2S, respectively. The scavenge of H2S reduced NO-mediated alleviation of HS suggesting of its downstream action in NO-mediated heat-tolerance. However, a simultaneous decrease of both (NO and H2S) led to higher Glc-mediated repression of photosynthesis and oxidative stress in terms of increased H2O2 content that was comparable to HS plants. Thus, NO and H2S cooperate to enhance photosynthesis under HS by reducing H2O2-induced oxidative stress and excess Glc-mediated photosynthetic suppression.
Investigations into the Role of Metabolism in the Inflammatory Response of BV2 Microglial Cells
Authors: Maher Pamela
Published: January 2021

Although the hallmarks of Alzheimer’s disease (AD) are amyloid beta plaques and neurofibrillary tangles, there is growing evidence that neuroinflammation, mitochondrial dysfunction and oxidative stress play important roles in disease development and progression. A major risk factor for the development of AD is diabetes, which is also characterized by oxidative stress and mitochondrial dysfunction along with chronic, low-grade inflammation. Increasing evidence indicates that in immune cells, the induction of a pro-inflammatory phenotype is associated with a shift from oxidative phosphorylation (OXPHOS) to glycolysis. However, whether hyperglycemia also contributes to this shift is not clear. Several different approaches including culturing BV2 microglial cells in different carbon sources, using enzyme inhibitors and knocking down key pathway elements were used in conjunction with bacterial lipopolysaccharide (LPS) activation to address this question. The results indicate that while high glucose favors NO production, pro-inflammatory cytokine production is highest in the presence of carbon sources that drive OXPHOS. In addition, among the carbon sources that drive OXPHOS, glutamine is a very potent inducer of IL6 production. This effect is dampened in the presence of glucose. Together, these results may provide new prospects for the therapeutic manipulation of neuroinflammation in the context of diabetes and AD.
General Environment Description Language
Authors: Zatwarnicki Krzysztof , Pokuta Waldemar , Bryniarska Anna , Zatwarnicka Anna , Metelski Andrzej , Piotrowska Ewelina
Published: January 2021

Artificial intelligence has been developed since the beginning of IT systems. Today there are many AI techniques that are successfully applied. Most of the AI field is, however, concerned with the so-called “narrow AI” demonstrating intelligence only in specialized areas. There is a need to work on general AI solutions that would constitute a framework enabling the integration of already developed narrow solutions and contribute to solving general problems. In this work, we present a new language that potentially can become a base for building intelligent systems of general purpose in the future. This language is called the General Environment Description Language (GEDL). We present the motivation for our research based on the other works in the field. Furthermore, there is an overall description of the idea and basic definitions of elements of the language. We also present an example of the GEDL language usage in the JSON notation. The example shows how to store the knowledge and define the problem to be solved, and the solution to the problem itself. In the end, we present potential fields of application and future work. This article is an introduction to new research in the field of Artificial General Intelligence.
Effect of Hydrogen Addition on the Energetic and Ecologic Parameters of an SI Engine Fueled by Biogas
Authors: Pukalskas Saugirdas , Kriaučiūnas Donatas , Rimkus Alfredas , Przybyła Grzegorz , Droździel Paweł , Barta Dalibor
Published: January 2021

The global policy solution seeks to reduce the usage of fossil fuels and greenhouse gas (GHG) emissions, and biogas (BG) represents a solutions to these problems. The use of biogas could help cope with increased amounts of waste and reduce usage of fossil fuels. Biogas could be used in compressed natural gas (CNG) engines, but the engine electronic control unit (ECU) needs to be modified. In this research, a spark ignition (SI) engine was tested for mixtures of biogas and hydrogen (volumetric hydrogen concentration of 0, 14, 24, 33, and 43%). In all experiments, two cases of spark timing (ST) were used: the first for an optimal mixture and the second for CNG. The results show that hydrogen increases combustion quality and reduces incomplete combustion products. Because of BG’s lower burning speed, the advanced ST increased brake thermal efficiency (BTE) by 4.3% when the engine was running on biogas. Adding 14 vol% of hydrogen (H2) increases the burning speed of the mixture and enhances BTE by 2.6% at spark timing optimal for CNG (CNG ST) and 0.6% at the optimal mixture ST (mixture ST). Analyses of the rate of heat release (ROHR), temperature, and pressure increase in the cylinder were carried out using utility BURN in AVL BOOST software.
Region Pixel Voting Network (RPVNet) for 6D Pose Estimation from Monocular Image
Authors: Xiong Feng , Liu Chengju , Chen Qijun
Published: January 2021

Recent studies have shown that deep learning achieves superior results in the task of estimating 6D-pose of target object from an image. End-to-end techniques use deep networks to predict pose directly from image, avoiding the limitations of handcraft features, but rely on training dataset to deal with occlusion. Two-stage algorithms alleviate this problem by finding keypoints in the image and then solving the Perspective-n-Point (PnP) problem to avoid directly fitting the transformation from image space to 6D-pose space. This paper proposes a novel two-stage method using only local features for pixel voting, called Region Pixel Voting Network (RPVNet). Front-end network detects target object and predicts its direction maps, from which the keypoints are recovered by pixel voting using Random Sample Consensus (RANSAC). The backbone, object detection network and mask prediction network of RPVNet are designed based on Mask R-CNN. Direction map is a vector field with the direction of each point pointing to its source keypoint. It is shown that predicting an object’s keypoints is related to its own pixels and independent of other pixels, which means the influence of occlusion decreases in the object’s region. Based on this phenomenon, in RPVNet, local features instead of the whole features, i.e., the output of the backbone, are used by a well-designed Convolutional Neural Networks (CNN) to compute direction maps. The local features are extracted from the whole features through RoIAlign, based on the region provided by detection network. Experiments on LINEMOD dataset show that RPVNet’s average accuracy (86.1%) is almost equal to state-of-the-art (86.4%) when no occlusion occurs. Meanwhile, results on Occlusion LINEMOD dataset show that RPVNet outperforms state-of-the-art (43.7% vs. 40.8%) and is more accurate for small object in occluded scenes.
Fully Superconducting Josephson Bolometers for Gigahertz Astronomy
Authors: Paolucci Federico , Ligato Nadia , Germanese Gaia , Buccheri Vittorio , Giazotto Francesco
Published: January 2021

The origin and the evolution of the universe are concealed in the evanescent diffuse extragalactic background radiation (DEBRA). To reveal these signals, the development of innovative ultra-sensitive bolometers operating in the gigahertz band is required. Here, we review the design and experimental realization of two bias-current-tunable sensors based on one dimensional fully superconducting Josephson junctions: the nanoscale transition edge sensor (nano-TES) and the Josephson escape sensor (JES). In particular, we cover the theoretical basis of the sensors operation, the device fabrication, their experimental electronic and thermal characterization and the deduced detection performance. Indeed, the nano-TES promises a state-of-the-art noise equivalent power (NEP) of about 5×10−20 W/Hz, while the JES active region is expected to show an unprecedented NEP of the order of 10−25 W/Hz. Therefore, the nano-TES and JES are strong candidates to push radio astronomy to the next level.
2-D Cross-Plot Model Analysis Using Integrated Geophysical Methods for Landslides Assessment
Authors: Zakaria Muhammad Taqiuddin , Mohd Muztaza Nordiana , Zabidi Hareyani , Salleh Alyaa Nadhira , Mahmud Nazirah , Samsudin Nuraisyah , Rosli Farid Najmi , Olugbenga Adeeko Tajudeen , Jia Teoh Ying
Published: January 2021

The large or small scale of a landslide is a natural, widespread process, resulting from the downward and outward movement of slope-forming materials, such as sculpting the landscape. Characterized landslide material and properties’ inhomogeneities conditions become a challenge as the process required the availability of a wide range of data, observations, and measurements with an evaluation of geological and hydrological conditions. Detailed investigations represent an essential component of the landslide risk mitigation process, relying on subsurface investigations, discrete subsurface sampling, and laboratory tests. To extend this approach, seismic refraction and two-dimensional (2-D) resistivity were utilized to study the landslides activities in Ulu Yam. The cross-plot analysis was introduced to integrate the geophysical results based on the criteria of the model. Velocity distributions from seismic refraction revealed the stiffness of the soil, where weak zones identified with values of Vp ≤ 1200 m/s, defined as threshold frequency for failure to occur. The 2-D resistivity shows that the weak zones were identified with resistivity values of <1200 Ωm. The 2-D cross-plot model gives a comprehensive interpretation where a low velocity and resistivity value represents the failure plane of materials to failure. The volume of mass sliding was calculated based on retrieved information from the model.
Phenotypic Definition and Genotype-Phenotype Correlates in PMPCA-Related Disease
Authors: Serpieri Valentina , Biagini Tommaso , Mazzotta Concetta , Pasquariello Rosa , Rubegni Anna , Santorelli Filippo , Ongari Gerardo , Cerri Silvia , Mazza Tommaso , Battini Roberta , Valente Enza Maria
Published: January 2021

Background: Peptidase mitochondrial processing alpha (PMPCA) biallelic mutations cause a spectrum of disorders ranging from severe progressive multisystemic mitochondrial encephalopathy to a milder non-progressive cerebellar ataxia with or without intellectual disability. Recently, we and others described an intermediate phenotype in two unrelated patients. Methods: We report a second Italian patient carrying novel PMPCA variants (p.Trp278Leu; p.Arg362Gly). Molecular modeling, dynamics simulation, RT-qPCR, and Western blotting were performed to predict the pathogenic impact of variants in the two Italian patients and attempt genotype-phenotype correlates. Results: In line with the two patients with intermediate phenotypes, our case presented global psychomotor delay with regression, intellectual disability, spastic-ataxic gait, and hyperkinetic movements, with cerebellar atrophy and bilateral striatal hyperintensities. However, blood lactate, muscle biopsy, and MRI spectroscopy were normal. PMPCA protein levels were significantly higher than controls despite normal cDNA levels. Dynamics simulation of several PMPCA missense variants showed a variable impact on the flexibility of the glycine rich loop and, for some cases, on the overall protein stability, without clear genotype-phenotype correlates. Conclusion: We confirm the expansion of PMPCA phenotypic spectrum including an intermediate phenotype of progressive encephalopathy without systemic involvement. The association of cerebellar atrophy with “Leigh-like” striatal hyperintensities may represent a “red flag” for this condition.
The Effects of Molecular Packing Behavior of Small-Molecule Acceptors in Ternary Organic Solar Cells
Authors: Lim Eunhee
Published: January 2021

Herein, two diketopyrrolopyrrole (DPP)-based, small-molecule isomers, o- and p-DPP-PhCN, were introduced as acceptors in ternary organic solar cells (OSCs). The isomers have the same molecular backbone but differ in the positions of the cyanide (CN) substituents (ortho and para), which greatly affects their packing behavior. Ternary solar cells composed of poly(3-hexylthiophene) (P3HT):DPP-PhCN:phenyl-C61-butyric acid methyl ester (PCBM) were fabricated, and the effects of the different packing behaviors of the third component on the device performance and the working mechanism of the ternary cells were investigated. The addition of o-DPP-PhCN with a relatively high-lying lowest unoccupied molecular orbital energy level resulted in an increase in the open-circuit voltage (VOC) in the ternary devices, demonstrating the alloy-like structure of the two acceptors (o-DPP-PhCN and PCBM) in the ternary system. However, the p-DPP-PhCN-based ternary cells exhibited VOC values similar to that of a P3HT:PCBM binary cell, irrespective of the addition of p-DPP-PhCN, indicating a cascade energy-level structure in the ternary system and an effective charge transfer from the P3HT to the PCBM. Importantly, by increasing the addition of p-DPP-PhCN, the short-circuit current density increased substantially, resulting in pronounced shoulder peaks in the external quantum efficiency responses in the long-wavelength region, corresponding to the contribution of the photocurrent generated by the light absorption of p-DPP-PhCN. Despite sharing the same molecular backbone, the two DPP-PhCNs exhibited substantially different packing behaviors according to the position of their CN substituents, which also greatly affected the working mechanism of the ternary devices fabricated using the DPP-PhCNs as the third component.
Ozone Variation Trends under Different CMIP6 Scenarios
Authors: Shang Lin , Luo Jiali , Wang Chunxiao
Published: January 2021

This study compares and analyzes simulations of ozone under different scenarios by three CMIP6 models (IPSL-CM6A, MRI-ESM2 and CESM-WACCM). Results indicate that as the social vulnerability and anthropogenic radiative forcing is increasing, the change of total column ozone in the tropical stratosphere is not linear. Compared to the SSP2-4.5 and SSP5-8.5 scenarios, the SSP1-2.6 and SSP3-7.0 are more favorable for the increase in stratospheric ozone mass in the tropics. Arctic ozone would never recover under the SSP1-2.6 scenario; however, the Antarctica ozone would gradually recover in all scenarios. Under the SSP1-2.6 and SSP2-4.5 scenarios, the trend of tropical total column ozone is mainly determined by the trend of column ozone in the tropical troposphere. Under the SSP3-7.0 scenario, tropospheric ozone concentration will significantly increase; under the SSP5-8.5 scenario, ozone concentration will distinctly increase in the middle and lower troposphere.