bluuebunny/crossref_metadata_2025 huggingface dataset:
GIS-Based Modeling of Human Settlement Suitability for the Belt and Road Regions
Authors: Li Wenjun , Li Peng , Feng Zhiming , Xiao Chiwei
Published: May 2022

(1) Background: Human settlements are a basic requirement for human survival and development. The natural suitability of human settlements directly affects human health and their quality of life and, to some extent, also dictates the size of the regional population and economy, as well as the level of urbanization. (2) Methods: This study presents a kilometer grid-based comprehensive human settlement suitability (HSS) evaluation model (containing the relief degree of the land surface (RDLS), the temperature–humidity index (THI), the land surface water abundance index (LSWAI), and the land cover index (LCI)). (3) Results: Based on the correlations between the four factors (i.e., the normalized RDLS (NRDLS), normalized THI (NTHI), LSWAI, and LCI), the NRDLS and NTHI were determined to be the leading factors, and the LSWAI and LCI were considered to be the auxiliary factors. The auxiliary factors were used to enhance the comprehensive HSS model determined by the leading factors. Based on this logic, spatial and index models were established separately. The HSS index for the BRI regions ranged from 0.07 to 1.00. Six levels of HSS were determined—namely, unsuitable, critically unsuitable, critically suitable, generally suitable, moderately suitable, and highly suitable conditions. In particular, the human settlements suitability is dominated by critically suitable and suitable conditions that accounted for 63% of the BRI. (4) Conclusions: The evaluation results of human settlements showed that topographic and climatic conditions are important limiting factors for the suitability of human settlements. Due to the renewability and manmade adjustability of hydrological and land cover conditions, the comprehensive suitability of human settlements shows differences in different geographic spaces along the BRI. The results provide a decision-making basis for the research on the resource carrying capacity and reasonable distribution of populations in the BRI, so as to realize the sustainable development along the regions.
The Relationship between Workplace Conflicts and Subsequent Physician-Certified Sick Leave: A Prospective Population Study
Authors: Sterud Tom , Marti Andrea R. , Degerud Eirik
Published: May 2022

The impact of workplace conflicts on sick leave is largely unknown. We studied the associations between conflicts and physician-certified sick leave in a randomly drawn general working population sample. Eligible respondents were interviewed in 2009, 2013, and 2016 and were registered with an employee relationship ≥50 working days in the national sick-leave register the year following the survey interviews (n = 22,088 observations/13,731 respondents). We used mixed-effects logistic regression models (adjusted for sex, age, education level, occupation and sick leave days) to assess the associations of self-reported conflicts with superiors or colleagues and subsequent physician-certified sick leave of 1–16 days (i.e., low-level sick leave (LLSL)) and more than 16 days (i.e., high-level sick leave (HLSL)). Conflicts with superiors were associated with LLSL (OR = 1.73 95% CI 1.15–2.62) and HLSL (OR = 1.84 95% CI 1.15–2.94). The corresponding ORs for conflicts involving colleagues were weaker and largely non-significant. The population risks of LLSL and HLSL attributable to conflicts with superiors were 1.95% (95% CI 0.55–3.41) and 3.98% (95% CI 2.08–5.91), respectively. Conflicts with superiors appear to be an important risk factor for sick leave among employees. Organizations are well-advised to develop policies and competencies to prevent and manage conflicts at work.
Efficacy of Combining an Extraoral High-Volume Evacuator with Preprocedural Mouth Rinsing in Reducing Aerosol Contamination Produced by Ultrasonic Scaling
Authors: Takenaka Shoji , Sotozono Maki , Yashiro Asaka , Saito Rui , Kornsombut Niraya , Naksagoon Traithawit , Nagata Ryoko , Ida Takako , Edanami Naoki , Noiri Yuichiro
Published: May 2022

The coronavirus disease pandemic has afforded dental professionals an opportunity to reconsider infection control during treatment. We investigated the efficacy of combining extraoral high-volume evacuators (eHVEs) with preprocedural mouth rinsing in reducing aerosol contamination by ultrasonic scalers. A double-masked, two-group, crossover randomized clinical trial was conducted over eight weeks. A total of 10 healthy subjects were divided into two groups; they received 0.5% povidone-iodine (PI), essential oil (EO), or water as preprocedural rinse. Aerosols produced during ultrasonic scaling were collected from the chest area (PC), dentist’s mask, dentist’s chest area (DC), bracket table, and assistant’s area. Bacterial contamination was assessed using colony counting and adenosine triphosphate assays. With the eHVE 10 cm away from the mouth, bacterial contamination by aerosols was negligible. With the eHVE 20 cm away, more dental aerosols containing bacteria were detected at the DC and PC. Mouth rinsing decreased viable bacterial count by 31–38% (PI) and 22–33% (EO), compared with no rinsing. The eHVE prevents bacterial contamination when close to the patient’s mouth. Preprocedural mouth rinsing can reduce bacterial contamination where the eHVE is positioned away from the mouth, depending on the procedure. Combining an eHVE with preprocedural mouth rinsing can reduce bacterial contamination in dental offices.
(Non)Marketing of Breastmilk Substitutes in South African Parenting Magazines: How Marketing Regulations May Be Working
Authors: Jewett Sara , Pilime Sukoluhle , Richter Linda
Published: May 2022

Although exclusive breastfeeding (EBF) for the first six months is optimal for child health, it remains low globally. Breastmilk substitutes (BMS) marketing undermines breastfeeding. In 2012, South Africa introduced Regulation 991, which prohibits marketing BMS products for infants below 6 months. Our study aimed to explore if and how BMS products were presented in South African parenting magazines post-R991. We applied a mixed-methods cross-sectional content analysis design, analyzing all 2018 issues of two popular parenting magazines. We descriptively analyzed quantitative codes, derived from an a priori framework, and conducted qualitative content analysis on a subset of texts and images. We found there was no overt marketing of BMS to parents with infants below 6 months. However, BMS advertisements were placed next to articles about young infants, and vague wording and images were ways by which BMS companies may indirectly benefit. Medical experts in both magazines promoted the introduction of solids before six months. To conclude, while BMS companies in South Africa were abiding by R991 by not overtly advertising BMS in parental print media, their influence persists. Continued monitoring of print media as well as other channels is advisable. This study may be of interest to countries considering stronger regulations of BMS advertising.
Associations between Family Functioning and Maternal Behavior on Default Mode Network Connectivity in School-Age Children
Authors: Rebello Keila , Moura Luciana Monteiro , Bueno Ana Paula Arantes , Picon Felipe Almeida , Pan Pedro Mario , Gadelha Ary , Miguel Euripedes Constatino , Bressan Rodrigo Affonseca , Rohde Luis Augusto , Sato João Ricardo
Published: May 2022

Background: Most early children’s experiences will occur in a family context; therefore, the quality of this environment is critical for development outcomes. Not many studies have assessed the correlations between brain functional connectivity (FC) in important areas such as the default mode network (DMN) and the quality of parent-child relationships in school-age children and early adolescence. The quality of family relationships and maternal behavior have been suggested to modulate DMN FC once they act as external regulators of children’s affect and behavior. Objective: We aimed to test the associations between the quality of family environment/maternal behavior and FC within the DMN of school-age children. Method: Resting-state, functional magnetic resonance imaging data, were collected from 615 children (6–12 age range) enrolled in the Brazilian High-Risk Cohort (HRC) study. We assessed DMN intra-connectivity between the medial prefrontal cortex (mPFC), posterior cingulate cortex (PCC), and inferior parietal lobule (IPL-bilateral) regions. The family functioning was assessed by levels of family cohesiveness and conflict and by maternal behavior styles such as maternal responsiveness, maternal stimulus to the child’s autonomy, and maternal overprotection. The family environment was assessed with the Family Environment Scale (FES), and maternal behavior was assessed by the mother’s self-report. Results: We found that the quality of the family environment was correlated with intra-DMN FC. The more conflicting the family environment was, the greater the FC between the mPFC-left IPL (lIPL), while a more cohesive family functioning was negatively correlated with FC between the PCC-lIPL. On the other hand, when moderated by a positive maternal behavior, cohesive family functioning was associated with increased FC in both regions of the DMN (mPFC-lIPL and PCC-lIPL). Conclusions: Our results highlight that the quality of the family environment might be associated with differences in the intrinsic DMN FC.
Rare Diseases in the Educational Field: Knowledge and Perceptions of Spanish Teachers
Authors: García-Perales Ramón , Palomares-Ruiz Ascensión , Ordóñez-García Lydia , García-Toledano Eduardo
Published: May 2022

Background: Education plays a fundamental role in everyone’s wellbeing. That means it is essential to provide quality inclusive activities to ensure equity and equality of opportunity in order to shape a cohesive, democratic, healthy society. Methods: In this study we focus on how inclusive educational practice addresses students with rare diseases, looking at teachers’ knowledge and opinions in this regard. A questionnaire was administered to 574 teachers who taught in various stages of non-university education to determine their knowledge and opinions about different dimensions: conceptualization, legislation, intervention, and diagnosis. Results: The results suggested various ideas for improvement in pursuit of positive, real inclusion, such as the need to improve teachers’ knowledge and understanding of these students’ characteristics and potential, with widespread specific training being urgently needed. Conclusions: in summary, students’ rights to education without discrimination is a basic premise of an educational system, leading to the need for a complete educational response that allows each student to develop as a person.
Using Smartphones for Indoor Fire Evacuation
Authors: Kuo Tzu-Wen , Lin Ching-Yuan , Chuang Ying-Ji , Hsiao Gary Li-Kai
Published: May 2022

Fire smoke reduces the visibility of emergency direction signs, rendering them ineffective for providing appropriate guidance along evacuation routes in a fire situation. This is problematic because civilians may select evacuation routes that expose them to smoke or fire in a burning building. This study proposed using a smartphone voice-guided evacuation system (SVGES) to provide alternative evacuation routes for civilians trapped at a fire scene. To verify the efficacy of the SVGES, experiments were conducted with 26 participants in a simulated fire scenario. The experimental results showed that when using the SVGES, the participants chose the safest evacuation route with a 100% successful evacuation rate.
Pan-Lysyl Oxidase Inhibitor PXS-5505 Ameliorates Multiple-Organ Fibrosis by Inhibiting Collagen Crosslinks in Rodent Models of Systemic Sclerosis
Authors: Yao Yimin , Findlay Alison , Stolp Jessica , Rayner Benjamin , Ask Kjetil , Jarolimek Wolfgang
Published: May 2022

Systemic sclerosis (SSc) is characterised by progressive multiple organ fibrosis leading to morbidity and mortality. Lysyl oxidases play a vital role in the cross-linking of collagens and subsequent build-up of fibrosis in the extracellular matrix. As such, their inhibition provides a novel treatment paradigm for SSc. A novel small molecule pan-lysyl oxidase inhibitor, PXS-5505, currently in clinical development for myelofibrosis treatment was evaluated using in vivo rodent models resembling the fibrotic conditions in SSc. Both lysyl oxidase and lysyl oxidase-like 2 (LOXL2) expression were elevated in the skin and lung of SSc patients. The oral application of PXS-5505 inhibited lysyl oxidase activity in the skin and LOXL2 activity in the lung. PXS-5505 exhibited anti-fibrotic effects in the SSc skin mouse model, reducing dermal thickness and α-smooth muscle actin. Similarly, in the bleomycin-induced mouse lung model, PXS-5505 reduced pulmonary fibrosis toward normal levels, mediated by its ability to normalise collagen/elastin crosslink formation. PXS-5505 also reduced fibrotic extent in models of the ischaemia-reperfusion heart, the unilateral ureteral obstruction kidney, and the CCl4-induced fibrotic liver. PXS-5505 consistently demonstrates potent anti-fibrotic efficacy in multiple models of organ fibrosis relevant to the pathogenesis of SSc, suggesting that it may be efficacious as a novel approach for treating SSc.
Design and Development of a New Type of Hybrid PLGA/Lipid Nanoparticle as an Ursolic Acid Delivery System against Pancreatic Ductal Adenocarcinoma Cells
Authors: Markowski Adam , Jaromin Anna , Migdał Paweł , Olczak Ewa , Zygmunt Adrianna , Zaremba-Czogalla Magdalena , Pawlik Krzysztof , Gubernator Jerzy
Published: May 2022

Despite many attempts, trials, and treatment procedures, pancreatic ductal adenocarcinoma (PDAC) still ranks among the most deadly and treatment-resistant types of cancer. Hence, there is still an urgent need to develop new molecules, drugs, and therapeutic methods against PDAC. Naturally derived compounds, such as pentacyclic terpenoids, have gained attention because of their high cytotoxic activity toward pancreatic cancer cells. Ursolic acid (UA), as an example, possesses a wide anticancer activity spectrum and can potentially be a good candidate for anti-PDAC therapy. However, due to its minimal water solubility, it is necessary to prepare an optimal nano-sized vehicle to overcome the low bioavailability issue. Poly(lactic-co-glycolic acid) (PLGA) polymeric nanocarriers seem to be an essential tool for ursolic acid delivery and can overcome the lack of biological activity observed after being incorporated within liposomes. PLGA modification, with the addition of PEGylated phospholipids forming the lipid shell around the polymeric core, can provide additional beneficial properties to the designed nanocarrier. We prepared UA-loaded hybrid PLGA/lipid nanoparticles using a nanoprecipitation method and subsequently performed an MTT cytotoxicity assay for AsPC-1 and BxPC-3 cells and determined the hemolytic effect on human erythrocytes with transmission electron microscopic (TEM) visualization of the nanoparticles and their cellular uptake. Hybrid UA-loaded lipid nanoparticles were also examined in terms of their stability, coating dynamics, and ursolic acid loading. We established innovative and repeatable preparation procedures for novel hybrid nanoparticles and obtained biologically active nanocarriers for ursolic acid with an IC50 below 20 µM, with an appropriate size for intravenous dosage (around 150 nm), high homogeneity of the sample (below 0.2), satisfactory encapsulation efficiency (up to 70%) and excellent stability. The new type of hybrid UA-PLGA nanoparticles represents a further step in the development of potentially effective PDAC therapies based on novel, biologically active, and promising triterpenoids.
Overexpression of ABI5 Binding Proteins Suppresses Inhibition of Germination Due to Overaccumulation of DELLA Proteins
Authors: Finkelstein Ruth R. , Lynch Tim J.
Published: May 2022

Abscisic acid (ABA) and gibberellic acid (GA) antagonistically regulate many aspects of plant growth, including seed dormancy and germination. The effects of these hormones are mediated by a complex network of positive and negative regulators of transcription. The DELLA family of proteins repress GA response, and can promote an ABA response via interactions with numerous regulators, including the ABA-insensitive (ABI) transcription factors. The AFP family of ABI5 binding proteins are repressors of the ABA response. This study tested the hypothesis that the AFPs also interact antagonistically with DELLA proteins. Members of these protein families interacted weakly in yeast two-hybrid and bimolecular fluorescence complementation studies. Overexpression of AFPs in sleepy1, a mutant that over-accumulates DELLA proteins, suppressed DELLA-induced overaccumulation of storage proteins, hyperdormancy and hypersensitivity to ABA, but did not alter the dwarf phenotype of the mutant. The interaction appeared to reflect additive effects of the AFPs and DELLAs, consistent with action in convergent pathways.
Machine-Learning-Based Genome-Wide Association Studies for Uncovering QTL Underlying Soybean Yield and Its Components
Authors: Yoosefzadeh-Najafabadi Mohsen , Eskandari Milad , Torabi Sepideh , Torkamaneh Davoud , Tulpan Dan , Rajcan Istvan
Published: May 2022

A genome-wide association study (GWAS) is currently one of the most recommended approaches for discovering marker-trait associations (MTAs) for complex traits in plant species. Insufficient statistical power is a limiting factor, especially in narrow genetic basis species, that conventional GWAS methods are suffering from. Using sophisticated mathematical methods such as machine learning (ML) algorithms may address this issue and advance the implication of this valuable genetic method in applied plant-breeding programs. In this study, we evaluated the potential use of two ML algorithms, support-vector machine (SVR) and random forest (RF), in a GWAS and compared them with two conventional methods of mixed linear models (MLM) and fixed and random model circulating probability unification (FarmCPU), for identifying MTAs for soybean-yield components. In this study, important soybean-yield component traits, including the number of reproductive nodes (RNP), non-reproductive nodes (NRNP), total nodes (NP), and total pods (PP) per plant along with yield and maturity, were assessed using a panel of 227 soybean genotypes evaluated at two locations over two years (four environments). Using the SVR-mediated GWAS method, we were able to discover MTAs colocalized with previously reported quantitative trait loci (QTL) with potential causal effects on the target traits, supported by the functional annotation of candidate gene analyses. This study demonstrated the potential benefit of using sophisticated mathematical approaches, such as SVR, in a GWAS to complement conventional GWAS methods for identifying MTAs that can improve the efficiency of genomic-based soybean-breeding programs.
YbdO Promotes the Pathogenicity of Escherichia coli K1 by Regulating Capsule Synthesis
Authors: Fan Yu , Sun Hongmin , Yang Wen , Bai Jing , Liu Peng , Huang Min , Guo Xi , Yang Bin , Feng Lu
Published: May 2022

Escherichia coli K1 is the most popular neonatal meningitis-causing Gram-negative bacterium. As a key virulence determinant, the K1 capsule enhances the survival of E. coli K1 in human brain microvascular endothelial cells (HBMECs) upon crossing the blood–brain barrier; however, the regulatory mechanisms of capsule synthesis during E. coli K1 invasion of HBMECs remain unclear. Here, we identified YbdO as a transcriptional regulator that promotes E. coli K1 invasion of HBMECs by directly activating K1 capsule gene expression to increase K1 capsule synthesis. We found that ybdO deletion significantly reduced HBMEC invasion by E. coli K1 and meningitis occurrence in mice. Additionally, electrophoretic mobility shift assay and chromatin immunoprecipitation–quantitative polymerase chain reaction analysis indicated that YbdO directly activates kpsMT and neuDBACES expression, which encode products involved in K1 capsule transport and synthesis by directly binding to the kpsM promoter. Furthermore, ybdO transcription was directly repressed by histone-like nucleoid structuring protein (H-NS), and we observed that acidic pH similar to that of early and late endosomes relieves this transcriptional repression. These findings demonstrated the regulatory mechanism of YbdO on K1 capsule synthesis, providing further insights into the evolution of E. coli K1 pathogenesis and host–pathogen interaction.
A Novel Human Neutralizing mAb Recognizes Delta, Gamma and Omicron Variants of SARS-CoV-2 and Can Be Used in Combination with Sotrovimab
Authors: Passariello Margherita , Ferrucci Veronica , Sasso Emanuele , Manna Lorenzo , Lembo Rosa Rapuano , Pascarella Stefano , Fusco Giovanna , Zambrano Nicola , Zollo Massimo , De Lorenzo Claudia
Published: May 2022

The dramatic experience with SARS-CoV-2 has alerted the scientific community to be ready to face new epidemics/pandemics caused by new variants. Among the therapies against the pandemic SARS-CoV-2 virus, monoclonal Antibodies (mAbs) targeting the Spike glycoprotein have represented good drugs to interfere in the Spike/ Angiotensin Converting Enzyme-2 (ACE-2) interaction, preventing virus cell entry and subsequent infection, especially in patients with a defective immune system. We obtained, by an innovative phage display selection strategy, specific binders recognizing different epitopes of Spike. The novel human antibodies specifically bind to Spike-Receptor Binding Domain (RBD) in a nanomolar range and interfere in the interaction of Spike with the ACE-2 receptor. We report here that one of these mAbs, named D3, shows neutralizing activity for virus infection in cell cultures by different SARS-CoV-2 variants and retains the ability to recognize the Omicron-derived recombinant RBD differently from the antibodies Casirivimab or Imdevimab. Since anti-Spike mAbs, used individually, might be unable to block the virus cell entry especially in the case of resistant variants, we investigated the possibility to combine D3 with the antibody in clinical use Sotrovimab, and we found that they recognize distinct epitopes and show additive inhibitory effects on the interaction of Omicron-RBD with ACE-2 receptor. Thus, we propose to exploit these mAbs in combinatorial treatments to enhance their potential for both diagnostic and therapeutic applications in the current and future pandemic waves of coronavirus.
Extracellular Polymeric Substances and Biocorrosion/Biofouling: Recent Advances and Future Perspectives
Authors: Wang Yanan , Zhang Ruiyong , Duan Jizhou , Shi Xin , Zhang Yimeng , Guan Fang , Sand Wolfgang , Hou Baorong
Published: May 2022

Microbial cells secrete extracellular polymeric substances (EPS) to adhere to material surfaces, if they get in contact with solid materials such as metals. After phase equilibrium, microorganisms can adhere firmly to the metal surfaces causing metal dissolution and corrosion. Attachment and adhesion of microorganisms via EPS increase the possibility and the rate of metal corrosion. Many components of EPS are electrochemical and redox active, making them closely related to metal corrosion. Functional groups in EPS have specific adsorption ability, causing them to play a key role in biocorrosion. This review emphasizes EPS properties related to metal corrosion and protection and the underlying microbially influenced corrosion (MIC) mechanisms. Future perspectives regarding a comprehensive study of MIC mechanisms and green methodologies for corrosion protection are provided.
Correlation between Sarcopenia Risk and Food Intake in Older Hospitalized Unselected Cancer Patients
Authors: Pimentel Gustavo D. , Borges Thaís C. , Gomes Tatyanne L. N.
Published: May 2022

Objectives: Recently, the SARC-F (Strength, Assistance for walking, Rise from a chair, Climb stairs, and Falls) questionnaire was developed to screen for the risk of sarcopenia in older patients. However, no study has investigated whether SARC-F is linked to food intake. This study aimed to evaluate the relationship between SARC-F and food intake in older hospitalized unselected cancer patients. Methods: A cross-sectional study included 53 older hospitalized unselected cancer patients. The SARC-F score was used to identify muscle function loss (SARC-F ≥ 4) or sarcopenia risk [SARC-F + calf circumference (CC) ≥ 11]. Pearson’s correlation was used to assess the relationship between SARC-F and food intake. Results: We found that 51% of patients presented with SARC-F ≥ 4 and 56.6% with SARC-F + CC ≥ 11. Although these patients had a lower calorie intake (22.4 ± 11.9 kcal/kg/day), they had an adequate distribution of macronutrients. We found a negative correlation between the SARC-F score and the calorie and macronutrient intake. However, SARC-F + CC was not correlated with calories and carbohydrates, only with lipid and protein intake. Conclusions: Approximately half of unselected cancer patients presented with muscle function loss (SARC-F ≥ 4) or sarcopenia risk (SARC-F + CC ≥ 11). In addition, we showed an inverse weak correlation between SARC-F and food intake, but not between SARC-F + CC and calories and carbohydrates, suggesting that the SARC-F questionnaire may be used with caution to screen for muscle function loss and correlation with food consumption.
The Influence of Rheumatoid Arthritis on Higher Reoperation Rates over Time Following Lumbar Spinal Fusion—A Nationwide Cohort Study
Authors: Park Jin-Sung , Park Se-Jun , Park Jiwon , Shin Gijun , Hong Jae-Young
Published: May 2022

This study aimed to compare the rates of reoperation over time following first lumbar fusion in rheumatoid arthritis (RA) patients and non-RA patients. This study was conducted using Korean Health Insurance Review and Assessment (HIRA) data. We identified the RA group as 2239 patients who underwent their first lumbar fusion with RA and the control group as 11,195 patients without RA. This reflects a ratio of 1:5, and the participants were matched by sex, age, and index surgery date. The index dates were between 2012 and 2013. When comparing the rate of patients undergoing reoperation, the adjusted HR was 1.31 (95% CI: 1.10–1.6) in the RA group (p = 0.002). In terms of the three time intervals, the values in the time frames of <3 months and 3 months–1 year were not statistically significant. However, at 1 year post-surgery, there was a higher risk of reoperation in the RA group, as demonstrated by the Kaplan–Meier cumulative event analysis. This higher risk of reoperation continued to increase throughout 5 years of follow-up, after which it was stable until the last follow-up at 7 years. This population-based cohort study showed that the RA patients had a 1.31 times higher risk of reoperation following lumbar fusion than did the controls. This difference was more pronounced at 1 year post-surgery.
Correction: Sugimoto et al. Trends in the Prevalence and Progression of Diabetic Retinopathy Associated with Hyperglycemic Disorders during Pregnancy in Japan. J. Clin. Med. 2022, 11, 165
Authors: Sugimoto Masahiko , Sampa Kohei , Tsukitome Hideyuki , Kato Kumiko , Matsubara Hisashi , Asami Shin , Sekimoto Kaori , Kitano Shigehiko , Yoshida Shigeo , Takamura Yoshihiro , Hirano Takao , Murata Toshinori , Shimizu Miho , Kinoshita Takamasa , Kusuhara Sentaro , Sawada Osamu , Ohji Masahito , Yoshikawa Rina , Kimura Kazuhiro , Ishikawa Hiroto , Gomi Fumi , Terasaki Hiroto , Kondo Mineo , Ikeda Tomoaki ,   on behalf of the Writing Committee of Japan-Clinical Retina STudy Group (J-CREST)
Published: May 2022

In the original publication [...]
A Longitudinal Retrospective Observational Study on Obesity Indicators and the Risk of Impaired Fasting Glucose in Pre- and Postmenopausal Women
Authors: Nam Myung Ji , Kim Hyunjin , Choi Yeon Joo , Cho Kyung-Hwan , Kim Seon Mee , Roh Yong-Kyun , Han Kyungdo , Jung Jin-Hyung , Park Yong-Gyu , Park Joo-Hyun , Kim Do-Hoon
Published: May 2022

The impact of obesity could differ according to menopausal status since women undergo significant physiologic and metabolic changes due to menopause. We investigated the association between various major obesity indicators and the risk of impaired fasting glucose (IFG) according to menopausal status using nationally representative data. A total of 571,286 premenopausal and 519,561 postmenopausal women who underwent both Korean National Health Insurance Service (NHIS) cancer screening in 2009 and health check-ups in 2017 were analyzed. Multivariate logistic regression analyses were used to assess the effect of independent variables of body mass index (BMI), waist circumference (WC), and waist-to-height ratio (WHtR) in 2009, on dependent variable IFG in 2017. After adjusting for potential confounders, the adjusted odds ratios (ORs) and 95% confidence intervals (CIs) of developing IFG were analyzed. In the premenopausal group, the OR of obese BMI (≥25 kg/m2, <30 kg/m2) women was increased to 2.228 (95% CI: 2.139–2.321) compared to the normal BMI (≥18.5, <23 kg/m2) women as a reference. In the postmenopausal group, there was also a higher OR of 1.778 (95% CI: 1.715–1.843) in the obese BMI women compared to the normal group. A similar association of increasing ORs for IFG was shown in both groups when stratified by WC and WHtR. This nationwide study revealed that obesity and abdominal obesity, defined by various obesity indicators, consistently increased odds of acquiring IFG after 8 years in both pre- and postmenopausal groups, with the association being more robust in the premenopausal group. Our findings suggest that weight management and lifestyle modification may require more attention in premenopausal women.
Special Issue “Advances in Gastrointestinal and Liver Disease: From Physiological Mechanisms to Clinical Practice”
Authors: Caviglia Gian Paolo , Ribaldone Davide Giuseppe
Published: May 2022

It is an exciting time for gastroenterology and hepatology [...]
Innovations in Shoulder Arthroplasty
Authors: Leafblad Nels , Asghar Elise , Tashjian Robert Z.
Published: May 2022

Innovations currently available with anatomic total shoulder arthroplasty include shorter stem designs and augmented/inset/inlay glenoid components. Regarding reverse shoulder arthroplasty (RSA), metal augmentation, including custom augments, on both the glenoid and humeral side have expanded indications in cases of bone loss. In the setting of revision arthroplasty, humeral options include convertible stems and newer tools to improve humeral implant removal. New strategies for treatment and surgical techniques have been developed for recalcitrant shoulder instability, acromial fractures, and infections after RSA. Finally, computer planning, navigation, PSI, and augmented reality are imaging options now available that have redefined preoperative planning and indications as well intraoperative component placement. This review covers many of the innovations in the realm of shoulder arthroplasty.
Biological Approach in the Treatment of External Popliteal Sciatic Nerve (Epsn) Neurological Injury: Review
Authors: León-Andrino Alejandro , Noriega David C. , Lapuente Juan P. , Pérez-Valdecantos Daniel , Caballero-García Alberto , Herrero Azael J. , Córdova Alfredo
Published: May 2022

The external popliteal sciatic nerve (EPSN) is the nerve of the lower extremity most frequently affected by compressive etiology. Its superficial and sinuous anatomical course is closely related to other rigid anatomical structures and has an important dynamic neural component. Therefore, this circumstance means that this nerve is exposed to multiple causes of compressive etiology. Despite this fact, there are few publications with extensive case studies dealing with treatment. In this review, we propose to carry out a narrative review of the neuropathy of the EPSN, including an anatomical reminder, its clinical presentation and diagnosis, as well as its surgical and biological approach. The most novel aspect we propose is the review of the possible role of biological factors in the reversal of this situation.
New Treatment Strategies for IgA Nephropathy: Targeting Plasma Cells as the Main Source of Pathogenic Antibodies
Authors: Maixnerova Dita , El Mehdi Delphine , Rizk Dana V. , Zhang Hong , Tesar Vladimir
Published: May 2022

Immunoglobulin A nephropathy (IgAN) is a rare autoimmune disorder and the leading cause of biopsy-reported glomerulonephritis (GN) worldwide. Disease progression is driven by the formation and deposition of immune complexes composed of galactose-deficient IgA1 (Gd-IgA1) and Gd-IgA1 autoantibodies (anti-Gd-IgA1 antibodies) in the glomeruli, where they trigger complement-mediated inflammation that can result in loss of kidney function and end-stage kidney disease (ESKD). With the risk of progression and limited treatment options, there is an unmet need for therapies that address the formation of pathogenic Gd-IgA1 antibody and anti-Gd-IgA1 antibody-containing immune complexes. New therapeutic approaches target immunological aspects of IgAN, including complement-mediated inflammation and pathogenic antibody production by inhibiting activation or promoting depletion of B cells and CD38-positive plasma cells. This article will review therapies, both approved and in development, that support the depletion of Gd-IgA1-producing cells in IgAN and have the potential to modify the course of this disease. Ultimately, we propose here a novel therapeutic approach by depleting CD38-positive plasma cells, as the source of the autoimmunity, to treat patients with IgAN.
A Generic Framework for Depth Reconstruction Enhancement
Authors: Sommerhoff Hendrik , Kolb Andreas
Published: May 2022

We propose a generic depth-refinement scheme based on GeoNet, a recent deep-learning approach for predicting depth and normals from a single color image, and extend it to be applied to any depth reconstruction task such as super resolution, denoising and deblurring, as long as the task includes a depth output. Our approach utilizes a tight coupling of the inherent geometric relationship between depth and normal maps to guide a neural network. In contrast to GeoNet, we do not utilize the original input information to the backbone reconstruction task, which leads to a generic application of our network structure. Our approach first learns a high-quality normal map from the depth image generated by the backbone method and then uses this normal map to refine the initial depth image jointly with the learned normal map. This is motivated by the fact that it is hard for neural networks to learn direct mapping between depth and normal maps without explicit geometric constraints. We show the efficiency of our method on the exemplary inverse depth-image reconstruction tasks of denoising, super resolution and removal of motion blur.
A Flexible Scheduling for Twin Yard Cranes at Container Terminals Considering Dynamic Cut-Off Time
Authors: Li Junjun , Yang Jingyu , Xu Bowei , Yin Wenjun , Yang Yongsheng , Wu Junfeng , Zhou Ye , Shen Yi
Published: May 2022

Yard handling is an important part of port logistics and affects the overall efficiency of the container port. The yard crane scheduling is affected by various external factors. For example, the dynamic cut-off time makes the release time of yard cranes variable, and the yard crane task arrangement will change frequently, resulting in a lot of computational time. To increase the flexibility of container yard handling, a twin yard cranes scheduling model is established considering the no-crossing constraints and the dynamic cut-off time. A joint scheduling of PSO and local re-scheduling strategy (LRPSO) is put forward to deal with the problem faster and more effectively. Small-scale and large-scale experiments are simulated to verify the performance of the proposed method. Results show that the scheduling method is more efficient.
Lack of Association between Fluconazole Susceptibility and ERG11 Nucleotide Polymorphisms in Cryptococcus neoformans Clinical Isolates from Uganda
Authors: Atim Priscilla Belbir , Meya David B. , Gerlach Elliot S. , Muhanguzi Dennis , Male Allan , Kanamwanji Benedict , Nielsen Kirsten
Published: May 2022

Fluconazole is the drug of choice for cryptococcal meningitis (CM) monoprophylaxis in resource-limited settings such as Uganda. Emerging fluconazole resistance linked to mutations in the Cryptococcus neoformansERG11 gene (CYP51) has been observed in clinical isolates. Currently, the single nucleotide polymorphisms [SNPs] in the Cryptococcus spp. ERG11 gene that could be responsible for fluconazole resistance are poorly characterized within Ugandan C. neoformans clinical isolates. If available, this information would be useful in the management of cryptococcosis among HIV patients. This cross-sectional study investigates the SNPs present in the coding region of the C. neoformansERG11 gene to determine the relationship between the SNPs identified and fluconazole susceptibility of the clinical isolates. 310 C. neoformans isolates recovered from the Cerebrospinal Fluid (CSF) of patients with HIV and cryptococcal meningitis were examined. The fluconazole half-maximal inhibitory concentrations (IC50 range: 0.25–32 μg/mL) was determined using the microbroth dilution method. A total of 56.1% of the isolates had low IC50 values of <8 μg/mL while 43.9% had high IC50 values ≥ 8 μg/mL. We amplified and sequenced 600 bp of the ERG11 coding sequence from 40 of the clinical isolates. Novel synonymous and 2 missense mutations, S460T and A457V, were identified in the ERG11 gene. The identified SNPs were not associated with differences in fluconazole IC50 values in vitro (p = 0.179).
Impact of the Arbuscular Mycorrhizal Fungus Funneliformis mosseae on the Physiological and Defence Responses of Canna indica to Copper Oxide Nanoparticles Stress
Authors: Luo Jie , Yan Qiuxia , Yang Guo , Wang Youbao
Published: May 2022

Copper oxide nanoparticles (nano-CuO) are recognized as an emerging pollutant. Arbuscular mycorrhizal fungi (AMF) can mitigate the adverse impacts of various pollutants on host plants. However, AMF’s mechanism for alleviating nano-CuO phytotoxicity remains unclear. The goal of this study was to evaluate how AMF inoculations affect the physiological features of Canna indica seedlings exposed to nano-CuO stress. Compared with the non-AMF inoculated treatment, AMF inoculations noticeably improved plant biomass, mycorrhizal colonization, leaf chlorophyll contents, and the photosynthetic parameters of C. indica under nano-CuO treatments. Moreover, AMF inoculation was able to significantly mitigate nano-CuO stress by enhancing antioxidant enzyme activities and decreasing ROS levels in the leaves and roots of C. indica, thus increasing the expression of genes involved in the antioxidant response. In addition, AMF inoculation reduced the level of Cu in seedlings and was associated with an increased expression of Cu transport genes and metallothionein genes. Furthermore, AMF inoculations increased the expression levels of organic acid metabolism-associated genes while facilitating organic acid secretion, thus reducing the accumulation of Cu. The data demonstrate that AMF–plant symbiosis is a feasible biocontrol approach to remediate nano-CuO pollution.
A Comprehensive Approach to Predicting the Outcomes of Transsphenoidal Endoscopic Adenomectomy in Patients with Cushing’s Disease
Authors: Kuritsyna Natalia V. , Tsoy Uliana A. , Cherebillo Vladislav Y. , Paltsev Artem A. , Ryzhkov Anton V. , Ryazanov Pavel A. , Ryzhkov Vladimir K. , Grineva Elena N.
Published: May 2022

Persistent and recurrent hypercortisolism after transsphenoidal endoscopic surgery (TSS) is considered to be an urgent issue prompting the search for Cushing’s disease (CD) remission predictors. The goal was to find a combination of predictors that can forecast the remission of CD after TSS. A total of 101 patients with CD who had undergone TSS were included. One year after surgery, CD remission status was evaluated. Preoperative pituitary magnetic resonance imaging (MRI) data, preoperative results of a high-dose dexamethasone suppression test (HDDST) and morning serum cortisol level collected 24 h after TSS (24 h MSeC) were compared in patients with and without remission of hypercortisolism. Remission one year after TSS was confirmed in 63 patients. CD remission predictors one year after TSS were: adenoma size ≥ 3 mm in the absence of invasive growth and the suppression of serum cortisol ≥ 74% in the HDDST, 24 h MSeC ≤ 388 nmol/L. A total of 38 patients had three favorable values of detected predictors; all of them had CD remission one year after TSS. With long-term follow-up, 36 of them remained in remission. Patients who had no one favorable predictor had no remission of hypercortisolism one year after TSS. Our data confirmed the prospects of using a combination of selected predictors to forecast CD remission after TSS.
Comparison of Pneumonia Severity Indices, qCSI, 4C-Mortality Score and qSOFA in Predicting Mortality in Hospitalized Patients with COVID-19 Pneumonia
Authors: Kibar Akilli Isil , Bilge Muge , Uslu Guz Arife , Korkusuz Ramazan , Canbolat Unlu Esra , Kart Yasar Kadriye
Published: May 2022

This is a retrospective and observational study on 1511 patients with SARS-CoV-2, who were diagnosed with COVID-19 by real-time PCR testing and hospitalized due to COVID-19 pneumonia. 1511 patients, 879 male (58.17%) and 632 female (41.83%) with a mean age of 60.1 ± 14.7 were included in the study. Survivors and non-survivors groups were statistically compared with respect to survival, discharge, ICU admission and in-hospital death. Although gender was not statistically significant different between two groups, 80 (60.15%) of the patients who died were male. Mean age was 72.8 ± 11.8 in non-survivors vs. 59.9 ± 14.7 in survivors (p < 0.001). Overall in-hospital mortality was found to be 8.8% (133/1511 cases), and overall ICU admission was 10.85% (164/1511 cases). The PSI/PORT score of the non-survivors group was higher than that of the survivors group (144.38 ± 28.64 versus 67.17 ± 25.63, p < 0.001). The PSI/PORT yielding the highest performance was the best predictor for in-hospital mortality, since it incorporates the factors as advanced age and comorbidity (AUROC 0.971; % 95 CI 0.961–0.981). The use of A-DROP may also be preferred as an easier alternative to PSI/PORT, which is a time-consuming evaluation although it is more comprehensive.
Diffusion of a Lifelog-Based Digital Healthcare Platform for Future Precision Medicine: Data Provision and Verification Study
Authors: Lee Kyuhee , Lee Jinhyong , Hwang Sangwon , Kim Youngtae , Lee Yeongjae , Urtnasan Erdenebayar , Koh Sang Baek , Youk Hyun
Published: May 2022

We propose a method for data provision, validation, and service expansion for the spread of a lifelog-based digital healthcare platform. The platform is an operational cloud-based platform, implemented in 2020, that has launched a tool that can validate and de-identify personal information in a data acquisition system dedicated to a center. The data acquired by the platform can be processed into products of statistical analysis and artificial intelligence (AI)-based deep learning modules. Application programming interfaces (APIs) have been developed to open data and can be linked in a programmatic manner. As a standardized policy, a series of procedures were performed from data collection to external sharing. The proposed platform collected 321.42 GB of data for 146 types of data. The reliability and consistency of the data were evaluated by an information system audit institution, with a defects ratio of approximately 0.03%. We presented definitions and examples of APIs developed in 17 functional units for data opening. In addition, the suitability of the de-identification tool was confirmed by evaluating the reduced risk of re-identification using quasi-identifiers. We presented specific methods for data verification, personal information de-identification, and service provision to ensure the sustainability of future digital healthcare platforms for precision medicine. The platform can contribute to the diffusion of the platform by linking data with external organizations and research environments in safe zones based on data reliability.
Measuring Quality of Life in Parkinson’s Disease—A Call to Rethink Conceptualizations and Assessments
Authors: Stührenberg Maria , Berghäuser Carolin S. , van Munster Marlena , Pedrosa Carrasco Anna J. , Pedrosa David J. ,   on behalf of the iCARE-PD Consortium
Published: May 2022

Parkinson’s disease (PD) is a chronic condition that considerably impacts the perception of quality of life (QoL) in both patients and their caregivers. Modern therapeutic approaches and social efforts strive at maintaining and promoting QoL. It has emerged as a fundamental parameter for clinical follow-up and poses one of the most important endpoints in scientific and economic evaluations of new care models. It is therefore of utmost importance to grasp concepts of QoL in a meaningful way. However, when taking a look at the origin of our modern understanding of QoL and existing methods for its measurement in PD patients, some aspects seem to lack sufficient appreciation. This article elaborates on how the perception of health and QoL have changed over time and discuss whether current understandings of both are reflected in the most commonly applied assessment methods for people with PD.
A System of Indicators for Socio-Economic Evaluation and Monitoring of Global Change: An Approach Based on the Picos de Europa National Park
Authors: López Iván , Suarez Rodrigo , Pardo Mercedes
Published: May 2022

National Parks are spaces that are of great interest for evaluating and monitoring global environmental change as these parks encompass natural, cultural, and rural features, along with ecological processes, which are subject to social or economic changes that are much more difficult to track outside of these spaces. To do this, it is necessary to have a sufficient set of data and indicators to monitor the effects of global change in the short, mid, and long term. The majority of indicators have been developed to monitor the bio-geophysical environment; socio-economic indicators of global change for National Parks are much more limited. The aim of this paper is to present a system of indicators for socio-economic evaluation and monitoring of global change for the Picos de Europa National Park. This park has two unique features: it has one of the two systems of socio-economic indicators developed for the Spanish National Parks, and it is practically the only one of Spain’s 16 National Parks with human populations living within its boundaries. Many of the indicators specifically developed for this park can be used for other national parks that have similar characteristics.
Risk Behavior Analysis in Indonesian Logistic Train Level Crossing
Authors: Restuputri Dian Palupi , Febriansyah Achmad Mahardhika , Masudin Ilyas
Published: May 2022

Background: At Indonesian level crossings, traversed by logistics trains, there are still frequent cases of accidents. The overall mishaps in 2020 were 199 cases of accidents at level crossings involving road drivers. Mental load factors affect the behavior of drivers on the road; Methods: this study, field observations and surveys were carried out using the Driver Behavior Question-naire (DBQ) and NASA TLX to measure mental load; Results: The results showed that 62% of drivers had a very high mental load factor. The mental load factors are effort, frustration, and temporal demand. Meanwhile, based on the results of DBQ, the type of behavior that is often carried out is Violation; Conclusions: From the results of field observations, it is also known that there is a significant influence between time and type of vehicle on violations. Thus, it was necessary to improve facilities and systems at level crossings to provide convenience and reduce the volume of transportation going through level crossings to reduce the risk of accidents and violations at level crossings.