bluuebunny/crossref_metadata_2025 huggingface dataset:
Finite Element Analysis on the Temperature- Dependent Burst Behavior of Domed 316L Austenitic Stainless Steel Rupture Disc
Authors: Zhu Hongbo , Xu Weipu , Luo Zhiping , Zheng Hongxing
Published: February 2020

As a safety device, a rupture disc instantly bursts as a nonreclosing pressure relief component to minimize the explosion risk once the internal pressure of vessels or pipes exceeds a critical level. In this study, the influence of temperature on the ultimate burst pressure of domed rupture discs made of 316L austenitic stainless steel was experimentally investigated and assessed with finite element analysis. Experimental results showed that the ultimate burst pressure gradually reduced from 6.88 MPa to 5.24 MPa with increasing temperature from 300 K to 573 K, which are consistent with the predicted instability pressures acquired by nonlinear buckling analysis using ABAQUS software. Additionally, it was found that a gradual transition from opening ductile mode to cleavage mode happened with increasing temperature due to more cross slips occurring under serious plastic deformation. The equivalent stress, equivalent strain and strain hardening rates acquired by static analysis were effective at rationalizing the temperature-dependent fracture behavior of the domed rupture discs.
New Approaches to Friction Stir Welding of Aluminum Light-Alloys
Authors: Cabibbo Marcello , Forcellese Archimede , Santecchia Eleonora , Paoletti Chiara , Spigarelli Stefano , Simoncini Michela
Published: February 2020

Friction stir welding (FSW) is the most widely used solid-state joining technique for light-weight plate and sheet products. This new joining technique is considered an energy-saving, environment friendly, and relatively versatile technology. FSW has been found to be a reliable joining technique in high-demand technology fields, such as high-strength aerospace aluminum and titanium alloys, and for other metallic alloys that are hard to weld by conventional fusion welding. Several studies accounted for the microstructural modifications induced by solid-state FSW, based on the resulting mechanical properties obtained at the FSW joints, such as tensile, bending, torsion, ductility and fatigue responses. In the last few years with the need and emerging urgency to widen the FSW application fields, broadening the possible alloy systems, and to optimize the resulting mechanical properties, this joining technique was further developed. In this respect, the present contribution focuses on two modified-FSW techniques and approaches applied to aluminum alloys plates. In a first case, an age-hardening AA6082 sheets were double side friction stir welded (DS-FSW). In a second case a non-age-hardening AA5754 sheet was FSW by an innovative approach in which welding pin was forced to slightly deviate away from the joining centreline (defined by authors as RT). In both the cases different pin heights were used, the sheets were subjected to heat treatments (peak hardening T6 for the AA6082, and annealing for the AA5754) and compared to the non-heat treated FSW conditions. Microstructural modifications were characterized by optical microscopy (OM). The mechanical properties were characterized both locally, by nanoindentation techniques, and globally, by tensile (yield, YT; ultimate, UT; and elongation, El) or forming limit curve (FLC) tests. Both the new approaches were directly compared to the conventional FSW techniques in terms of resulting microstructures and mechanical responses.
Characterization of Fungal Endophytes Isolated from the Metal Hyperaccumulator Plant Vachellia farnesiana Growing in Mine Tailings
Authors: Salazar-Ramírez Giovanni , Flores-Vallejo Rosario del Carmen , Rivera-Leyva Julio César , Tovar-Sánchez Efraín , Sánchez-Reyes Ayixon , Mena-Portales Julio , Sánchez-Carbente María del Rayo , Gaitán-Rodríguez María Fernanda , Batista-García Ramón Alberto , Villarreal María Luisa , Mussali-Galante Patricia , Folch-Mallol Jorge Luis
Published: February 2020

Heavy metal pollution has become an environmental and health problem worldwide. With the aim of finding novel strategies for metal bioremediation, endophytic fungi from the heavy metal hyperaccumulator plant Vachellia farnesiana were isolated and characterized. The plants were growing in mine tailings, rich in Zn, Pb, and Cu. Morphological and phylogenetic analyses indicated that the fungal strains belonged to Neocosmospora and Aspergillus genera. The Neocosmospora isolate belongs to the Fusarium solani species complex (FSSC) that groups phytopathogen species. However, in this case the plants from which it was isolated did not show any signs of disease. Both fungal strains were able to remove significant amounts of heavy metals from liquid cultures, either in a mixture of the three metals or each metal in a single culture. In response to lead exposure, the Neocosmospora sp. strain secreted specific novel phenolic compounds other than anthraquinones or naphtoquinones, which have been described in similar situations. The Aspergillus sp. dropped the pH in the medium. High-performance liquid chromatography determinations indicated that this strain secreted mainly glutamic acid in response to lead, a novel mechanism, which has not been reported elsewhere. Malic and succinic acids were also produced in response to lead exposure. Possibly, glutamic and succinic acids (synthesized in the Krebs cycle) can be used to cope with metal toxicity due to the plant providing photosynthates to the fungus. These fungi showed the potential to be used for bioremediation or restoration of metal-polluted environments.
Cording Mycobacterium tuberculosis Bacilli Have a Key Role in the Progression towards Active Tuberculosis, Which is Stopped by Previous Immune Response
Authors: Arias Lilibeth , Cardona Paula , Català Martí , Campo-Pérez Víctor , Prats Clara , Vilaplana Cristina , Julián Esther , Cardona Pere-Joan
Published: February 2020

Cording was the first virulence factor identified in Mycobacterium tuberculosis (Mtb). We aimed to ascertain its role in the induction of active tuberculosis (TB) in the mouse strain C3HeB/FeJ by testing the immunopathogenic capacity of the H37Rv strain. We have obtained two batches of the same strain by stopping their growth in Proskauer Beck liquid medium once the mid-log phase was reached, in the noncording Mtb (NCMtb) batch, and two days later in the cording Mtb (CMtb) batch, when cording could be detected by microscopic analysis. Mice were challenged with each batch intravenously and followed-up for 24 days. CMtb caused a significant increase in the bacillary load at an early stage post-challenge (day 17), when a granulomatous response started, generating exudative lesions characterized by neutrophilic infiltration, which promoted extracellular bacillary growth together with cording formation, as shown for the first time in vivo. In contrast, NCMtb experienced slight or no bacillary growth and lesions could barely be detected. Previous Bacillus Calmette-Guérin (BCG) vaccination or low dose aerosol (LDA) Mtb infection were able to delay the progression towards active TB after CMtb challenge. While BCG vaccination also reduced bacillary load when NCMtb was challenged, LDA did not, and its proliferative lesions experienced neutrophil infiltration. Analysis of lung cytokine and chemokine profiles points to their capacity to block the production of CXCL-1 and further amplification of IL-1β, IL-17 and neutrophilic extracellular trap formation, all of which are essential for TB progression. These data highlight the key role of cording formation in the induction of active TB.
Synchrony Degree of Dietary Energy and Nitrogen Release Influences Microbial Community, Fermentation, and Protein Synthesis in a Rumen Simulation System
Authors: Zhang Jun , Zheng Nan , Shen Weijun , Zhao Shengguo , Wang Jiaqi
Published: February 2020

Synchrony of energy and nitrogen release in rumen has been proposed to maximize ruminal microbial fermentation. However, the information regarding bacterial community composition and its metabolism under a higher or lower degree of synchronization is limited. In our study, a 0 to 6 h post-feeding infusion (first half infusion, FHI), 6 to 12 h post-feeding infusion (second half infusion, SHI), and 0 to 12 h post-feeding infusion (continuous infusion, CI) of maltodextrin were used to simulate varying degrees of synchronization of energy and nitrogen release in a rumen simulation system. In addition, the bacterial community, metabolite, enzyme activity, and microbial protein synthesis (MPS) were evaluated. Compared with the FHI and CI, the relative abundance of Fibrobacter, Ruminobacter, BF311, and CF231 decreased in the SHI, but that of Klebsiella and Succinivibrio increased in the SHI. The NH3-N and branched-chain volatile fatty acids were significantly higher, but propionate content and activities of glutamate dehydrogenase (GDH) and alanine dehydrogenase were significantly lower in the SHI than those in the FHI and CI. The SHI had lower MPS and less efficiency of MPS than the FHI and CI, which indicated that the SHI had a lower degree of synchronization. Correlation analysis showed that MPS was positively related to GDH activity and relative abundance of Fibrobacter but negatively related to NH3-N and relative abundance of Klebsiella. Therefore, a higher degree of synchronization of energy and nitrogen release increased MPS partly via influencing the bacterial community, metabolism, and enzyme activities of ammonia assimilation in the in vitro fermenters.
A Synchrotron Mössbauer Spectroscopy Study of a Hydrated Iron-Sulfate at High Pressures
Authors: Perez Tyler , Finkelstein Gregory J. , Pardo Olivia , Solomatova Natalia V. , Jackson Jennifer M.
Published: February 2020

Szomolnokite is a monohydrated ferrous iron sulfate mineral, FeSO4·H2O, where the ferrous iron atoms are in octahedral coordination with four corners shared with SO4 and two with H2O groups. While somewhat rare on Earth, szomolnokite has been detected on the surface of Mars along with several other hydrated sulfates and is suggested to occur near the surface of Venus. Previous measurements have characterized the local environment of the iron atoms in szomolnokite using Mössbauer spectroscopy at a range of temperatures and 1 bar. Our study represents a step towards understanding the electronic environment of iron in szomolnokite under compression at 300 K. Using a hydrostatic helium pressure-transmitting medium, we explored the pressure dependence of iron’s site-specific behavior in a synthetic szomolnokite powdered sample up to 95 GPa with time-domain synchrotron Mössbauer spectroscopy. At 1 bar, the Mössbauer spectrum is well described by two Fe2+-like sites and no ferric iron, consistent with select conventional Mössbauer spectra evaluations. At pressures below 19 GPa, steep gradients in the hyperfine parameters are most likely due to a structural phase transition. At 19 GPa, a fourth site is required to explain the time spectrum with increasing fractions of a low quadrupole splitting site, which could indicate the onset of another transition. Above 19 GPa we present three different models, including those with a high- to low-spin transition, that provide reasonable scenarios of electronic environment changes of the iron in szomolnokite with pressure. We summarize the complex range of Fe2+ spin transition characteristics at high-pressures by comparing szomolnokite with previous studies on ferrous-iron bearing phases.
Spider Silk for Tissue Engineering Applications
Authors: Salehi Sahar , Koeck Kim , Scheibel Thomas
Published: February 2020

Due to its properties, such as biodegradability, low density, excellent biocompatibility and unique mechanics, spider silk has been used as a natural biomaterial for a myriad of applications. First clinical applications of spider silk as suture material go back to the 18th century. Nowadays, since natural production using spiders is limited due to problems with farming spiders, recombinant production of spider silk proteins seems to be the best way to produce material in sufficient quantities. The availability of recombinantly produced spider silk proteins, as well as their good processability has opened the path towards modern biomedical applications. Here, we highlight the research on spider silk-based materials in the field of tissue engineering and summarize various two-dimensional (2D) and three-dimensional (3D) scaffolds made of spider silk. Finally, different applications of spider silk-based materials are reviewed in the field of tissue engineering in vitro and in vivo.
Ultrasound and Radiation-Induced Catalytic Oxidation of 1-Phenylethanol to Acetophenone with Iron-Containing Particulate Catalysts
Authors: Soliman Mohamed M. A. , Kopylovich Maximilian N. , Alegria Elisabete C. B. A. , Ribeiro Ana P. C. , M. Ferraria Ana , M. Botelho do Rego Ana , Correia Luís M. M. , Saraiva Marta S. , Pombeiro Armando J. L.
Published: February 2020

Iron-containing particulate catalysts of 0.1–1 µm size were prepared by wet and ball-milling procedures from common salts and characterized by FTIR, TGA, UV-Vis, PXRD, FEG-SEM, and XPS analyses. It was found that when the wet method was used, semi-spherical magnetic nanoparticles were formed, whereas the mechanochemical method resulted in the formation of nonmagnetic microscale needles and rectangles. Catalytic activity of the prepared materials in the oxidation of 1-phenylethanol to acetophenone was assessed under conventional heating, microwave (MW) irradiation, ultrasound (US), and oscillating magnetic field of high frequency (induction heating). In general, the catalysts obtained by wet methods exhibit lower activities, whereas the materials prepared by ball milling afford better acetophenone yields (up to 83%). A significant increase in yield (up to 4 times) was observed under the induction heating if compared to conventional heating. The study demonstrated that MW, US irradiations, and induction heating may have great potential as alternative ways to activate the catalytic system for alcohol oxidation. The possibility of the synthesized material to be magnetically recoverable has been also verified.
Sterically Stabilised Polymeric Mesoporous Silica Nanoparticles Improve Doxorubicin Efficiency: Tailored Cancer Therapy
Authors: Moodley Thashini , Singh Moganavelli
Published: February 2020

The fruition, commercialisation and clinical application combining nano-engineering, nanomedicine and material science for utilisation in drug delivery is becoming a reality. The successful integration of nanomaterial in nanotherapeutics requires their critical development to ensure physiological and biological compatibility. Mesoporous silica nanoparticles (MSNs) are attractive nanocarriers due to their biodegradable, biocompatible, and relative malleable porous frameworks that can be functionalized for enhanced targeting and delivery in a variety of disease models. The optimal formulation of an MSN with polyethylene glycol (2% and 5%) and chitosan was undertaken, to produce sterically stabilized, hydrophilic MSNs, capable of efficient loading and delivery of the hydrophobic anti-neoplastic drug, doxorubicin (DOX). The pH-sensitive release kinetics of DOX, together with the anticancer, apoptosis and cell-cycle activities of DOX-loaded MSNs in selected cancer cell lines were evaluated. MSNs of 36–60 nm in size, with a pore diameter of 9.8 nm, and a cumulative surface area of 710.36 m2/g were produced. The 2% pegylated MSN formulation (PCMSN) had the highest DOX loading capacity (0.98 mgdox/mgmsn), and a sustained release profile over 72 h. Pegylated-drug nanoconjugates were effective at a concentration range between 20–50 μg/mL, inducing apoptosis in cancer cells, and affirming their potential as effective drug delivery vehicles.
The Pharmaceutical Industry in 2019. An Analysis of FDA Drug Approvals from the Perspective of Molecules
Authors: de la Torre Beatriz G. , Albericio Fernando
Published: February 2020

During 2019, the US Food and Drug Administration (FDA) approved 48 new drugs (38 New Chemical Entities and 10 Biologics). Although this figure is slightly lower than that registered in 2018 (59 divided between 42 New Chemical Entities and 17 Biologics), a year that broke a record with respect to new drugs approved by this agency, it builds on the trend initiated in 2017, when 46 drugs were approved. Of note, three antibody drug conjugates, three peptides, and two oligonucleotides were approved in 2019. This report analyzes the 48 new drugs of the class of 2019 from a strictly chemical perspective. The classification, which was carried out on the basis of chemical structure, includes the following: Biologics (antibody drug conjugates, antibodies, and proteins); TIDES (peptide and oligonucleotides); drug combinations; natural products; and small molecules.
Analytical Approaches for Analysis of Safety of Modern Food Packaging: A Review
Authors: Wrona Magdalena , Nerín Cristina
Published: February 2020

Nowadays, food packaging is a crucial tool for preserving food quality and has become an inseparable part of our daily life. Strong consumer demand and market trends enforce more advanced and creative forms of food packaging. New packaging development requires safety evaluations that always implicate the application of complex analytical methods. The present work reviews the development and application of new analytical methods for detection of possible food contaminants from the packaging origin on the quality and safety of fresh food. Among food contaminants migrants, set-off migrants from printing inks, polymer degradation products, and aromatic volatile compounds can be found that may compromise the safety and organoleptic properties of food. The list of possible chemical migrants is very wide and includes antioxidants, antimicrobials, intentionally added substances (IAS), non-intentionally added substances (NIAS), monomers, oligomers, and nanoparticles. All this information collected prior to the analysis will influence the type of analyzing samples and molecules (analytes) and therefore the selection of a convenient analytical method. Different analytical strategies will be discussed, including techniques for direct polymer analysis.
Electroactive Ultra-Thin rGO-Enriched FeMoO4 Nanotubes and MnO2 Nanorods as Electrodes for High-Performance All-Solid-State Asymmetric Supercapacitors
Authors: Ranjith Kugalur Shanmugam , Raju Ganji Seeta Rama , R. Chodankar Nilesh , Ghoreishian Seyed Majid , Kwak Cheol Hwan , Huh Yun Suk , Han Young-Kyu
Published: February 2020

A flexible asymmetric supercapacitor (ASC) with high electrochemical performance was constructed using reduced graphene oxide (rGO)-wrapped redox-active metal oxide-based negative and positive electrodes. Thin layered rGO functionality on the positive and the negative electrode surfaces has promoted the feasible surface-active sites and enhances the electrochemical response with a wide operating voltage window. Herein we report the controlled growth of rGO-wrapped tubular FeMoO4 nanofibers (NFs) via electrospinning followed by surface functionalization as a negative electrode. The tubular structure offers the ultrathin-layer decoration of rGO inside and outside of the tubular walls with uniform wrapping. The rGO-wrapped tubular FeMoO4 NF electrode exhibited a high specific capacitance of 135.2 F g−1 in Na2SO4 neutral electrolyte with an excellent rate capability and cycling stability (96.45% in 5000 cycles) at high current density. Meanwhile, the hydrothermally synthesized binder-free rGO/MnO2 nanorods on carbon cloth (rGO-MnO2@CC) were selected as cathode materials due to their high capacitance and high conductivity. Moreover, the ASC device was fabricated using rGO-wrapped FeMoO4 on carbon cloth (rGO-FeMoO4@CC) as the negative electrode and rGO-MnO2@CC as the positive electrode (rGO-FeMoO4@CC/rGO-MnO2@CC). The rationally designed ASC device delivered an excellent energy density of 38.8 W h kg−1 with a wide operating voltage window of 0.0–1.8 V. The hybrid ASC showed excellent cycling stability of 93.37% capacitance retention for 5000 cycles. Thus, the developed rGO-wrapped FeMoO4 nanotubes and MnO2 nanorods are promising hybrid electrode materials for the development of wide-potential ASCs with high energy and power density.
Metal-Based Nanoparticles as Antimicrobial Agents: An Overview
Authors: Sánchez-López Elena , Gomes Daniela , Esteruelas Gerard , Bonilla Lorena , Lopez-Machado Ana Laura , Galindo Ruth , Cano Amanda , Espina Marta , Ettcheto Miren , Camins Antoni , Silva Amélia M. , Durazzo Alessandra , Santini Antonello , Garcia Maria L. , Souto Eliana B.
Published: February 2020

Metal-based nanoparticles have been extensively investigated for a set of biomedical applications. According to the World Health Organization, in addition to their reduced size and selectivity for bacteria, metal-based nanoparticles have also proved to be effective against pathogens listed as a priority. Metal-based nanoparticles are known to have non-specific bacterial toxicity mechanisms (they do not bind to a specific receptor in the bacterial cell) which not only makes the development of resistance by bacteria difficult, but also broadens the spectrum of antibacterial activity. As a result, a large majority of metal-based nanoparticles efficacy studies performed so far have shown promising results in both Gram-positive and Gram-negative bacteria. The aim of this review has been a comprehensive discussion of the state of the art on the use of the most relevant types of metal nanoparticles employed as antimicrobial agents. A special emphasis to silver nanoparticles is given, while others (e.g., gold, zinc oxide, copper, and copper oxide nanoparticles) commonly used in antibiotherapy are also reviewed. The novelty of this review relies on the comparative discussion of the different types of metal nanoparticles, their production methods, physicochemical characterization, and pharmacokinetics together with the toxicological risk encountered with the use of different types of nanoparticles as antimicrobial agents. Their added-value in the development of alternative, more effective antibiotics against multi-resistant Gram-negative bacteria has been highlighted.
Stakeholder’s Perceptions of Mexico’s Federal Corn Flour Fortification Program: A Qualitative Study
Authors: Waller Anna W. , Dominguez-Uscanga Astrid , Lopez Barrera Emely , Andrade Juan E. , Andrade Jeanette M.
Published: February 2020

Background: In Mexico, the fortification of corn and wheat flours with iron, zinc, and folic acid and the restoration of B-vitamins is a mandatory program. However, the monitoring and evaluation (M&E) of this fortification process is not well understood. Thus, the purpose of the study was to understand the M&E of the food fortification program in Mexico, with an emphasis on technology research and development. Methods: Open-ended exploratory interviews were conducted with food technology representatives (n = 9), food science academic faculty (n = 1), president of a private tortilla-making federation (n = 1), and representatives of the federal monitoring agency (n = 2). Interviews were transcribed and themes were identified using the content analysis methodology. Inter-rater reliability was assessed by calculating an intraclass correlation coefficient (ICC) between the raters (n = 3). Results: A total of 49 codes were identified that resulted in three overarching themes, manufacturing/processing, monitoring logistics, and nutrition. Overall, there is a need for more robust internal and external M&E with Mexico’s fortification program to improve the manufacturing/processing of fortifying the tortillas, the monitoring of this fortification program, and the impact the fortified tortillas have on the nutritional status of the Mexican population. The overall ICC was 0.87. Conclusions: The present study can be used to gain insight into Mexico’s fortification program and to inform food fortification policymakers of best practices.
The Cow’s Milk-Related Symptom Score (CoMiSSTM): Health Care Professional and Parent and Day-to-Day Variability
Authors: Vandenplas Yvan , Carvajal Eva , Peeters Stefaan , Balduck Nadine , Jaddioui Yesra , Ribes-Koninckx Carmen , Huysentruyt Koen
Published: February 2020

The Cow’s Milk-related Symptom Score (CoMiSSTM) was created as an awareness tool for cow’s milk allergy. The aim of the present study was to analyze the inter-rater variability between a pediatrician, parents, and day to day variability. A Health Care Professional (HCP) and parent filled in the CoMiSS independently and blinded for each other to evaluate inter-rater variability. In order to validate day-to-day variability, a parent filled in the CoMiSS during 3 consecutive days and was compared to the CoMiSS scored by the HCP. The absolute agreement between parent and HCP was 75%, and 92.6% and 100% with a tolerance of 0, 1, and 2 points, respectively, resulting in excellent agreement with an intraclass correlation coefficient (ICC) 0.981 (95% Confidence Interval 0.974–0.986, p < 0.001). Day-to-day variability during 3 consecutive days resulted in an absolute agreement of 30%, increasing to 80% and 88.6% when 2 and 3 points, respectively, were accepted. The ICC was excellent for the parental prospective scores (0.93, 95% CI 0.90–0.96; p < 0.001). Day-to-day variability indicates that CoMiSS has a moderate inter-rater reliability. A very low variability was observed when scored prospectively over three days. Data suggest that the CoMiSS can reliably be scored by parents without additional training.
The DOF Transcription Factors in Seed and Seedling Development
Authors: Ruta Veronica , Longo Chiara , Lepri Andrea , De Angelis Veronica , Occhigrossi Sara , Costantino Paolo , Vittorioso Paola
Published: February 2020

The DOF (DNA binding with one finger) family of plant-specific transcription factors (TF) was first identified in maize in 1995. Since then, DOF proteins have been shown to be present in the whole plant kingdom, including the unicellular alga Chlamydomonas reinhardtii. The DOF TF family is characterised by a highly conserved DNA binding domain (DOF domain), consisting of a CX2C-X21-CX2C motif, which is able to form a zinc finger structure. Early in the study of DOF proteins, their relevance for seed biology became clear. Indeed, the PROLAMIN BINDING FACTOR (PBF), one of the first DOF proteins characterised, controls the endosperm-specific expression of the zein genes in maize. Subsequently, several DOF proteins from both monocots and dicots have been shown to be primarily involved in seed development, dormancy and germination, as well as in seedling development and other light-mediated processes. In the last two decades, the molecular network underlying these processes have been outlined, and the main molecular players and their interactions have been identified. In this review, we will focus on the DOF TFs involved in these molecular networks, and on their interaction with other proteins.
Soybean Resistance to Soybean Mosaic Virus
Authors: Widyasari Kristin , Alazem Mazen , Kim Kook-Hyung
Published: February 2020

Soybean mosaic virus (SMV) occurs in all soybean-growing areas in the world and causes huge losses in soybean yields and seed quality. During early viral infection, molecular interactions between SMV effector proteins and the soybean resistance (R) protein, if present, determine the development of resistance/disease in soybean plants. Depending on the interacting strain and cultivar, R-protein in resistant soybean perceives a specific SMV effector, which triggers either the extreme silent resistance or the typical resistance manifested by hypersensitive responses and induction of salicylic acid and reactive oxygen species. In this review, we consider the major advances that have been made in understanding the soybean–SMV arms race. We also focus on dissecting mechanisms SMV employs to establish infection and how soybean perceives and then responds to SMV attack. In addition, progress on soybean R-genes studies, as well as those addressing independent resistance genes, are also addressed.
Melatonin Application Improves Salt Tolerance of Alfalfa (Medicago sativa L.) by Enhancing Antioxidant Capacity
Authors: Cen Huifang , Wang Tingting , Liu Huayue , Tian Danyang , Zhang Yunwei
Published: February 2020

Alfalfa (Medicago sativa L.) is an important and widely cultivated forage grass. The productivity and forage quality of alfalfa are severely affected by salt stress. Melatonin is a bioactive molecule with versatile physiological functions and plays important roles in response to various biotic and abiotic stresses. Melatonin has been proven efficient in improving alfalfa drought and waterlogging tolerance in recent studies. In our reports, we applied melatonin exogenously to explore the effects of melatonin on alfalfa growth and salt resistance. The results demonstrated that melatonin application promoted alfalfa seed germination and seedling growth, and reduced oxidative damage under salt stress. Further application research found that melatonin alleviated salt injury in alfalfa plants under salt stress. The electrolyte leakage, malondialdehyde (MDA) content and H2O2 content were significantly reduced, and the activities of catalase (CAT), peroxidase (POD), and Cu/Zn superoxide dismutase (Cu/Zn-SOD) were increased with melatonin pretreatment compared to control plants under salt stress with the upregulation of genes related to melatonin and antioxidant enzymes biosynthesis. Melatonin was also involved in reducing Na+ accumulation in alfalfa plants. Our study indicates that melatonin plays a primary role as an antioxidant in scavenging H2O2 and enhancing activities of antioxidant enzymes to improve the salt tolerance of alfalfa plants.
Reducing Cadmium Accumulation in Plants: Structure–Function Relations and Tissue-Specific Operation of Transporters in the Spotlight
Authors: Huang Xin , Duan Songpo , Wu Qi , Yu Min , Shabala Sergey
Published: February 2020

Cadmium (Cd) is present in many soils and, when entering the food chain, represents a major health threat to humans. Reducing Cd accumulation in plants is complicated by the fact that most known Cd transporters also operate in the transport of essential nutrients such as Zn, Fe, Mn, or Cu. This work summarizes the current knowledge of mechanisms mediating Cd uptake, radial transport, and translocation within the plant. It is concluded that real progress in the field may be only achieved if the transport of Cd and the above beneficial micronutrients is uncoupled, and we discuss the possible ways of achieving this goal. Accordingly, we suggest that the major focus of research in the field should be on the structure–function relations of various transporter isoforms and the functional assessment of their tissue-specific operation. Of specific importance are two tissues. The first one is a xylem parenchyma in plant roots; a major “controller” of Cd loading into the xylem and its transport to the shoot. The second one is a phloem tissue that operates in the last step of a metal transport. Another promising and currently underexplored avenue is to understand the role of non-selective cation channels in Cd uptake and reveal mechanisms of their regulation.
Performance of Coarse Graining in Estimating Polymer Properties: Comparison with the Atomistic Model
Authors: Miwatani Ryota , Takahashi Kazuaki Z. , Arai Noriyoshi
Published: February 2020

Combining atomistic and coarse-grained (CG) models is a promising approach for quantitative prediction of polymer properties. However, the gaps between the length and time scales of atomistic and CG models still need to be bridged. Here, the scale gaps of the atomistic model of polyethylene melts, the bead–spring Kremer–Grest model, and dissipative particle dynamics with the slip-spring model were investigated. A single set of spatial and temporal scaling factors was determined between the atomistic model and each CG model. The results of the CG models were rescaled using the set of scaling factors and compared with those of the atomistic model. For each polymer property, a threshold value indicating the onset of static or dynamic universality of polymers was obtained. The scaling factors also revealed the computational efficiency of each CG model with respect to the atomistic model. The performance of the CG models of polymers was systematically evaluated in terms of both the accuracy and computational efficiency.
Fluoroalkyl Pentacarbonylmanganese(I) Complexes as Initiators for the Radical (co)Polymerization of Fluoromonomers
Authors: Morales-Cerrada Roberto , Ladmiral Vincent , Gayet Florence , Fliedel Christophe , Poli Rinaldo , Améduri Bruno
Published: February 2020

The use of [Mn(RF)(CO)5] (RF = CF3, CHF2, CH2CF3, COCF2CH3) to initiate the radical polymerization of vinylidene fluoride (F2C=CH2, VDF) and the radical alternating copolymerization of vinyl acetate (CH2=CHOOCCH3, VAc) with tert-butyl 2-(trifluoromethyl)acrylate (MAF-TBE) by generating primary RF• radicals is presented. Three different initiating methods with [Mn(CF3)(CO)5] (thermal at ca. 100 °C, visible light and UV irradiations) are described and compared. Fair (60%) to satisfactory (74%) polyvinylidene fluoride (PVDF) yields were obtained from the visible light and UV activations, respectively. Molar masses of PVDF reaching 53,000 g·mol−1 were produced from the visible light initiation after 4 h. However, the use of [Mn(CHF2)(CO)5] and [Mn(CH2CF3)(CO)5] as radical initiators produced PVDF in a very low yield (0 to 7%) by both thermal and photochemical initiations, while [Mn(COCF2CH3)(CO)5] led to the formation of PVDF in a moderate yield (7% to 23%). Nevertheless, complexes [Mn(CH2CF3)(CO)5] and [Mn(COCHF2)(CO)5] efficiently initiated the alternating VAc/MAF-TBE copolymerization. All synthesized polymers were characterized by 1H and 19F NMR spectroscopy, which proves the formation of the expected PVDF or poly(VAc-alt-MAF-TBE) and showing the chaining defects and the end-groups in the case of PVDF. The kinetics of VDF homopolymerization showed a linear ln[M]0/[M] versus time relationship, but a decrease of molar masses vs. VDF conversion was noted in all cases, which shows the absence of control. These PVDFs were rather thermally stable in air (up to 410 °C), especially for those having the highest molar masses. The melting points ranged from 164 to 175 °C while the degree of crystallinity varied from 44% to 53%.
Probing the Dynamics of Li+ Ions on the Crystal Surface: A Solid-State NMR Study
Authors: Wang Bi-Heng , Xia Tian , Chen Qun , Yao Ye-Feng
Published: February 2020

Polyethylene oxide-based solid polymer electrolytes (SPEs) are of research interest because of their potential applications in all-solid-state Li+ batteries. However, despite their advantages in terms of compatibility with the electrodes and easy processing, polyethylene oxide (PEO)/Li+ complexes often suffer from low conductivity at room temperature. Understanding the conduction mechanism and, in turn, developing strategies to improve the conductivity have long been the main objectives underlying research into PEO/Li+ complex electrolytes. Here, we prepared several special PEO/Li+ complex samples where the PEO/Li+ complex structures were located on the surfaces of PEO crystals and consisted of high content chain ends. We found two different Li+ species in the PEO/Li+ complex structures via solid-state nuclear magnetic resonance (NMR). The 2D 7Li exchange NMR showed the exchange process between the different Li+ species. The exchange dynamics of the Li+ ions provide a molecular mechanism of the Li+ transportation in the surface of PEO crystal lamella, which is further correlated with the ionic conduction mechanism of the PEO/Li+ complex structure.
Utilization of Water-Soluble Aminoethylamino–β–Cyclodextrin in the Pfitzinger Reaction—Catalyzed to the Synthesis of Diversely Functionalized Quinaldine
Authors: Kim Yohan , Shinde Vijay Vilas , Jeong Daham , Jung Seunho
Published: February 2020

In this study we describe the use of an aminoethylamino-β-cyclodextrin (AEA–β–CD) as a supramolecular homogeneous catalyst for the synthesis of a series of diversely substituted quinaldine derivatives which are medicinally important, via Pfitzinger reaction. This supramolecular catalyst exhibited remarkable catalytic activity with high substrate scope to achieve the synthetic targets in good to excellent yield, 69–92%. The structural and morphological properties of the synthesized AEA–β–CD were determined through MALDI–TOF mass spectrometry, NMR, FT-IR, and SEM analysis. Possible reaction mechanisms were determined through molecular host–guest complexation and proposed based on 2D NMR (ROESY) spectroscopy, FT-IR, FE-SEM, and DSC.
Heat Exchanger Network Synthesis Integrated with Compression–Absorption Cascade Refrigeration System
Authors: Sun Xiaojing , Liu Linlin , Zhuang Yu , Zhang Lei , Du Jian
Published: February 2020

Compression–absorption cascade refrigeration system (CACRS) is the extension of absorption refrigeration system, which can be utilized to recover excess heat of heat exchanger networks (HENs) and compensate refrigeration demand. In this work, a stage-wise superstructure is presented to integrate the generation and evaporation processes of CACRS within HEN, where the generator is driven by hot process streams, and the evaporation processes provide cooling energy to HEN. Considering that the operating condition of CACRS has significant effect on the coefficient of performance (COP) of CACRS and so do the structure of HEN, CACRS and HEN are considered as a whole system in this study, where the operating condition and performance of CACRS and the structure of HEN are optimized simultaneously. The quantitative relationship between COP and operating variables of CACRS is determined by process simulation and data fitting. To accomplish the optimal design purpose, a mixed integer non-linear programming (MINLP) model is formulated according to the proposed superstructure, with the objective of minimizing total annual cost (TAC). At last, two case studies are presented to demonstrate that desired HEN can be achieved by applying the proposed method, and the results show that the integrated HEN-CACRS system is capable to utilize energy reasonably and reduce the total annualized cost by 38.6% and 37.9% respectively since it could recover waste heat from hot process stream to produce the cooling energy required by the system.
High-Frequency Variations in Pearl River Plume Observed by Soil Moisture Active Passive Sea Surface Salinity
Authors: Liao Xiaomei , Du Yan , Wang Tianyu , Hu Shuibo , Zhan Haigang , Liu Huizeng , Wu Guofeng
Published: February 2020

River plumes play an important role in the cross-margin transport of phytoplankton and nutrients, which have profound impacts on coastal ecosystems. Using recently available Soil Moisture Active Passive (SMAP) sea surface salinity (SSS) data and high-resolution ocean color products, this study investigated summertime high-frequency variations in the Pearl River plume of China and its biological response. The SMAP SSS captures the intraseasonal oscillations in the offshore transport of the Pearl River plume well, which has distinct 30–60 day variations from mid-May to late September. The offshore transport of freshwater varies concurrently with southwesterly wind anomalies and is roughly in phase with the Madden–Julian Oscillation (MJO) index in phases 1–5, thus implying that the MJO exerts a significant influence. During MJO phases 1–2, the southwest wind anomalies in the northeastern South China Sea (SCS) enhanced cross-shore Ekman transport, while the northeast wind anomalies during MJO phases 3–5 favored the subsequent southwestward transport of the plume. The high chlorophyll-a concentration coincided well with the low-salinity water variations, emphasizing the important role of the offshore transport of the Pearl River plume in sustaining biological production over the oligotrophic northern SCS. The strong offshore transport of the plume in June 2015 clearly revealed that the proximity of a cyclonic eddy plays a role in the plume’s dispersal pathway. In addition, heavy rainfall related to the landfall of tropical cyclones in the Pearl River Estuary region contributed to the episodic offshore transport of the plume.
Geometric Accuracy Improvement Method for High-Resolution Optical Satellite Remote Sensing Imagery Combining Multi-Temporal SAR Imagery and GLAS Data
Authors: Zhu Quansheng , Jiang Wanshou , Zhu Ying , Li Linze
Published: February 2020

With the widespread availability of satellite data, a single region can be described using multi-source and multi-temporal remote sensing data, such as high-resolution (HR) optical imagery, synthetic aperture radar (SAR) imagery, and space-borne laser altimetry data. These have become the main source of data for geopositioning. However, due to the limitation of the direct geometric accuracy of HR optical imagery and the effect of the small intersection angle of HR optical imagery in stereo pair orientation, the geometric accuracy of HR optical imagery cannot meet the requirements for geopositioning without ground control points (GCPs), especially in uninhabited areas, such as forests, plateaus, or deserts. Without satellite attitude error, SAR usually provides higher geometric accuracy than optical satellites. Space-borne laser altimetry technology can collect global laser footprints with high altitude accuracy. Therefore, this paper presents a geometric accuracy improvement method for HR optical satellite remote sensing imagery combining multi-temporal SAR Imagery and GLAS data without GCPs. Based on the imaging mechanism, the differences in the weight matrix determination of the HR optical imagery and SAR imagery were analyzed. The laser altimetry data with high altitude accuracy were selected and applied as height control point in combined geopositioning. To validate the combined geopositioning approach, GaoFen2 (GF2) optical imagery, GaoFen6 (GF6) optical imagery, GaoFen3 (GF3) SAR imagery, and the Geoscience Laser Altimeter System (GLAS) footprint were tested. The experimental results show that the proposed model can be effectively applied to combined geopositioning to improve the geometric accuracy of HR optical imagery. Moreover, we found that the distribution and weight matrix determination of SAR images and the distribution of GLAS footprints are the crucial factors influencing geometric accuracy. Combined geopositioning using multi-source remote sensing data can achieve a plane accuracy of 1.587 m and an altitude accuracy of 1.985 m, which is similar to the geometric accuracy of geopositioning of GF2 with GCPs.
Evapotranspiration in the Tono Reservoir Catchment in Upper East Region of Ghana Estimated by a Novel TSEB Approach from ASTER Imagery
Authors: Alhassan Abdullah , Jin Menggui
Published: February 2020

Evapotranspiration (ET) is dynamic and influences water resource distribution. Sustainable management of water resources requires accurate estimations of the individual components that result in evapotranspiration, including the daily net radiation (DNR). Daily ET is more useful than the evaporative fraction (EF) provided by remote sensing ET models, and to account for daily variations, EF is usually combined with the DNR. DNR exhibits diurnal and spatiotemporal variations due to landscape heterogeneity. In the modified Two-Source Energy Balance (TSEB) approach by Zhuang and Wu, 2015, ecophysiological constraint functions of temperature and moisture of plants based on atmospheric moisture and vegetation indices were introduced, but the DNR was not spatially accounted for in the estimation of the daily ET. This research adopted a novel approach that accounts for spatiotemporal variations in estimated daily ET by incorporating the Bisht and Bras DNR model in the modified version of the TSEB model. Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) satellite imagery over the Tono irrigation watershed within the Upper East Region of Ghana and Southern Burkina Faso were used. We estimated the energy fluxes of latent and sensible heat as well as the net radiation and soil heat fluxes from the satellite images and compared our results with ground-based measurements from an eddy covariance (EC) station established by the West African Science Service Center on Climate Change and Adapted Land Use (WASCAL) within the watershed area. We noticed a similarity between our model estimated fluxes and ET with the ground-based EC station measurements. Eight different land use/cover types were identified in the study area, and each of these contributed significantly to the overall ET variations between the two study periods: December 2009 and December 2017. For instance, due to a higher leaf area index (LAI) for all vegetation types in December 2009 than in December 2017, the ET for December 2017 was higher than that for December 2009. We also noticed that the land use/cover types within the footprint area of the EC station were only six out of the eight. Generally, all the surface energy fluxes increased from December 2009 to December 2017. Mean ET varied from 3.576 to 4.486 (mm/d) for December 2009 while from 4.502 to 5.280 (mm/d) for December 2017 across the different land use/cover classes. Knowledge of the dynamics of evapotranspiration and adoption of cost-effective methods to estimate its individual components in an effective and efficient way is critical to water resources management. Our findings provide a tool for all water stakeholders within watersheds to manage water resources in an engaging and cost-effective way.
Assessment of Multi-Scale SMOS and SMAP Soil Moisture Products across the Iberian Peninsula
Authors: Portal Gerard , Jagdhuber Thomas , Vall-llossera Mercè , Camps Adriano , Pablos Miriam , Entekhabi Dara , Piles Maria
Published: February 2020

In the last decade, technological advances led to the launch of two satellite missions dedicated to measure the Earth’s surface soil moisture (SSM): the ESA’s Soil Moisture and Ocean Salinity (SMOS) launched in 2009, and the NASA’s Soil Moisture Active Passive (SMAP) launched in 2015. The two satellites have an L-band microwave radiometer on-board to measure the Earth’s surface emission. These measurements (brightness temperatures TB) are then used to generate global maps of SSM every three days with a spatial resolution of about 30–40 km and a target accuracy of 0.04 m3/m3. To meet local applications needs, different approaches have been proposed to spatially disaggregate SMOS and SMAP TB or their SSM products. They rely on synergies between multi-sensor observations and are built upon different physical assumptions. In this study, temporal and spatial characteristics of six operational SSM products derived from SMOS and SMAP are assessed in order to diagnose their distinct features, and the rationale behind them. The study is focused on the Iberian Peninsula and covers the period from April 2015 to December 2017. A temporal inter-comparison analysis is carried out using in situ SSM data from the Soil Moisture Measurements Station Network of the University of Salamanca (REMEDHUS) to evaluate the impact of the spatial scale of the different products (1, 3, 9, 25, and 36 km), and their correspondence in terms of temporal dynamics. A spatial analysis is conducted for the whole Iberian Peninsula with emphasis on the added-value that the enhanced resolution products provide based on the microwave-optical (SMOS/ERA5/MODIS) or the active–passive microwave (SMAP/Sentinel-1) sensor fusion. Our results show overall agreement among time series of the products regardless their spatial scale when compared to in situ measurements. Still, higher spatial resolutions would be needed to capture local features such as small irrigated areas that are not dominant at the 1-km pixel scale. The degree to which spatial features are resolved by the enhanced resolution products depend on the multi-sensor synergies employed (at TB or soil moisture level), and on the nature of the fine-scale information used. The largest disparities between these products occur in forested areas, which may be related to the reduced sensitivity of high-resolution active microwave and optical data to soil properties under dense vegetation.