bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization of uranyl (UO22+) ion binding to amyloid beta (Aβ) peptides: effects on Aβ structure and aggregation
Authors: Berntsson Elina , Vosough Faraz , Noormägi Andra , Padari Kärt , Asplund Fanny , Gielnik Maciej , Paul Suman , Jarvet Jüri , Tõugu Vello , Roos Per M. , Kozak Maciej , Gräslund Astrid , Barth Andreas , Pooga Margus , Palumaa Peep , Wärmländer Sebastian K. T. S.
Published: April 2023

Abstract

Uranium (U) is naturally present in ambient air, water, and soil, and depleted uranium (DU) is released into the environment via industrial and military activities. While the radiological damage from U is rather well understood, less is known about the chemical damage mechanisms, which dominate in DU. Heavy metal exposure is associated with numerous health conditions including Alzheimer’s disease (AD), the most prevalent age-related cause of dementia. The pathological hallmark of AD is deposition of amyloid plaques, consisting mainly of amyloid-β (Aβ) peptides aggregated into amyloid fibrils in the brain. However, the toxic species in AD are likely oligomeric Aβ aggregates. Exposure to heavy metals such as Cd, Hg, Mn, and Pb is known to increase Aβ production, and these metals bind to Aβ peptides and modulate their aggregation. Possible effects of U in AD pathology have been sparsely studied. Here, we use biophysical techniques to studyin vitrointeractions between Aβ peptides and uranyl ions, UO22+, of DU. We show for the first time that uranyl ions bind to Aβ peptides with affinities in the micromolar range, induce structural changes in Aβ monomers and oligomers, and inhibit Aβ fibrillization. General toxic mechanisms of uranyl ions could be modulation of protein folding, misfolding, and aggregation.
Characterization of the Root-Associated Microbiome Provides Insights into Endemism ofThymusSpecies Growing in the Kazdagi National Park
Authors: Ercan Gökçe , Arıkan Muzaffer , Yüzbaşıoğlu İ. Sırrı , Yüzbaşıoğlu F. Elif Çepni
Published: April 2023

Plant associated microbiomes have a large impact on the fitness of the plants in the particular environmental conditions. The root associated microbiomes are shaped by the interactions between the microbial community members, their plant host, and environmental factors. Hence, further understanding of the composition and functions of the plant root associated microbiomes can pave the way for the development of more effective conservation strategies for endangered endemic plants. Here, we characterized the bacterial and fungal microbiomes in bulk and rhizosphere soil of an endemic and a non-endemicThymusspecies from Kazdagi National Park, Türkiye,Thymus pulvinatusandThymus longicaulissubsp.chaubardii, respectively, by 16S rRNA gene and ITS amplicon sequencing. Our findings revealed no significant differences in alpha diversity between plant species and soil types. However, we found that the bacterial microbiome profiles differentiate not onlyThymusspecies but also soil types while fungal microbiome profiles show distinct profiles particularly between the species in beta diversity.Proteobacteria, Actinobacteria, Acidobacteria, andChlorofleximembers form the core bacterial microbiome while the fungal core microbiome consists ofAscomycotaandBasidiomycotamembers in bothThymusspecies. Moreover, we identified the association of the bacterial taxa contributing to the biogeochemical cycles of carbon and nitrogen and providing the stress resistance with the rhizosphere soil of endemicT. pulvinatus. In addition, functional predictions suggested distinct enriched functions in rhizosphere soil samples of the two plant species. Also, employing an exploratory integrative analysis approach, we determined the plant species-specific nature of transkingdom interactions in twoThymusspecies.
Recombulator-X: a fast and user-friendly tool for estimating X chromosome recombination rates in forensic genetics
Authors: Aneli Serena , Fariselli Piero , Chierto Elena , Bini Carla , Robino Carlo , Birolo Giovanni
Published: April 2023

Background and Objective

Genetic markers (especially short tandem repeats or STRs) located on the X chromosome are a valuable resource to solve complex kinship cases in forensic genetics in addition or alternatively to autosomal STRs. Groups of tightly linked markers are combined into haplotypes, thus increasing the discriminating power of tests. However, this approach requires precise knowledge of the recombination rates between adjacent markers.

Recombination rates vary across the human genome and cannot be automatically derived from linkage physical maps. The International Society of Forensic Genetics recommends that recombination rate estimation on the X chromosome is performed from pedigree genetic data while taking into account the confounding effect of mutations. However, the only existing implementations that satisfy these requirements have several drawbacks: they were never publicly released, they are very slow and/or need cluster-level hardware and strong computational expertise to use.

In order to address these key concerns, we developed Recombulator-X, a new open-source Python tool.

Methods

The most challenging issue, namely the running time, was addressed with dynamic programming techniques to greatly reduce the computational complexity of the algorithm, coupled with JIT compilation to further increase performance. We also extended the statistical framework from STR to any polymorphic marker.

Results

Compared to the previous methods, Recombulator-X reduces the estimation times from weeks or months to less than one hour for typical datasets. Moreover, the estimation process, including preprocessing, has been streamlined and packaged into a simple command-line tool that can be run on a normal PC.

Where previous approaches were limited to small panels of STR markers (up to 15), our tool can handle greater numbers (up to 100) of mixed STR and non-STR markers.

Conclusions

In the genetic forensic community, state-of-the-art estimation methods for X chromosome recombination rates have seen limited usage due to the technical hurdles posed by previous implementations. Recombulator-X makes the process much simpler, faster and accessible to researchers without a computational background, hopefully spurring increased adoption of best practices. Moreover, it extends the estimation framework to larger panels of genetic markers (not only STRs), allowing analyses of sequencing-based data.
Dysregulated Wnt and NFAT signaling in a Parkinson’s disease LRRK2 G2019S knock-in model
Authors: Wetzel Andrea , Lei Si Hang , Liu Tiansheng , Hughes Michael P. , Peng Yunan , McKay Tristan , Waddington Simon N. , Grannò Simone , Rahim Ahad A. , Harvey Kirsten
Published: April 2023

Background

Parkinson’s disease (PD) is a progressive late-onset neurodegenerative disease leading to physical and cognitive decline. Mutations of leucine-rich repeat kinase 2 (LRRK2) are the most common genetic cause of PD. LRRK2 is a complex scaffolding protein with known regulatory roles in multiple molecular pathways. Two prominent examples of LRRK2-modulated pathways are Wingless/Int (Wnt) and nuclear factor of activated T-cells (NFAT) signaling. Both are well described key regulators of immune and nervous system development as well as maturation. The aim of this study was to establish the physiological and pathogenic role of LRRK2 in Wnt and NFAT signaling in the brain, as well as the potential contribution of the non-canonical Wnt/Calcium pathway.

Methods

In vivocerebral Wnt and NFATc1 signaling activity was quantified in LRRK2 G2019S mutant knock-in (KI) and LRRK2 knockout (KO) male and female mice with repeated measures over 28 weeks, employing lentiviral luciferase biosensors, and analyzed using a mixed-effect model. To establish spatial resolution, we investigated tissues, and primary neuronal cell cultures from different brain regions combining luciferase signaling activity, immunohistochemistry, qPCR and western blot assays. Results were analyzed by unpaired t-test with Welch’s correction or 2-way ANOVA with post hoc corrections.

Results

In vivoWnt signaling activity in LRRK2 KO and LRRK2 G2019S KI mice was increased significantly ∼3-fold, with a more pronounced effect in males (∼4-fold) than females (∼2-fold). NFATc1 signaling was reduced ∼0.5-fold in LRRK2 G2019S KI mice. Brain tissue analysis showed region-specific expression changes in Wnt and NFAT signaling components. These effects were predominantly observed at the protein level in the striatum and cerebral cortex of LRRK2 KI mice. Primary neuronal cell culture analysis showed significant genotype-dependent alterations in Wnt and NFATc1 signaling under basal and stimulated conditions. Wnt and NFATc1 signaling was primarily dysregulated in cortical and hippocampal neurons respectively.

Conclusions

Our study further built on knowledge of LRRK2 as a Wnt and NFAT signaling protein. We identified complex changes in neuronal models of LRRK2 PD, suggesting a role for mutant LRRK2 in the dysregulation of NFAT, and canonical and non-canonical Wnt signaling.
5’-NADylation of RNA is well-tolerated by bacterial cell
Authors: Wiedermannova Jana , Babu Ravishankar , Yuzenkova Yulia
Published: April 2023

Recently discovered ability of various RNA polymerases (RNAPs) of bacteria and eukaryotes to initiate RNA synthesis with cofactors such as NAD+/NADH (nicotinamide adenine dinucleotide). This phenomenon was quickly dubbed “non-canonical capping” as the extra 5’ moieties of RNA resemble eukaryotic m7G cap superficially. NAD must outcompete abundant ATP, to serve as initiating nucleotide. It is still unclear which physiological conditions permit significant RNA NADylation and to what degree NADylation serves typical functions of a canonical cap in bacteria.

Here we found that the extent of NADyation depends primarily on fidelity of transcription initiation –NAD concentration, specific contacts of RNAP active site with NAD/ATP alternative substrates, and genomic DNA supercoiling. It is much less affected by posttranscriptional processes such as de-capping, processing by main 5’-dependent ribonucleases RNaseE and oligoribonuclease. 5’-NAD inhibits neither translation of a leaderless 5’-NAD-RNA nor a physiological base-pairing of small regulatory NADylated RNA with its antisense target RNA. Translation exposes 5’-NAD for de-capping by NudC enzyme.
A role for cytoglobin in regulating intracellular hydrogen peroxide and redox signals in the vasculature
Authors: Mathai Clinton , Jourd’heuil Frances , Pham Le Gia Cat , Gilliard Kurrim , Balnis Joseph , Jen Annie , Overmyer Katherine A. , Coon Joshua J , Jaitovich Ariel , Boivin Benoit , Jourd’heuil David
Published: April 2023

The oxidant hydrogen peroxide serves as a signaling molecule that alters many aspects of cardiovascular functions. Recent studies suggest that cytoglobin – a hemoglobin expressed in the vasculature – may promote electron transfer reactions with proposed functions in hydrogen peroxide decomposition. Here, we determined the extent to which cytoglobin regulates intracellular hydrogen peroxide and established mechanisms. We found that cytoglobin decreased the hyperoxidation of peroxiredoxins and maintained the activity of peroxiredoxin 2 following challenge with exogenous hydrogen peroxide. Cytoglobin promoted a reduced intracellular environment and facilitated the reduction of the thiol-based hydrogen peroxide sensor Hyper7 after bolus addition of hydrogen peroxide. Cytoglobin also limited the inhibitory effect of hydrogen peroxide on glycolysis and reversed the oxidative inactivation of the glycolytic enzyme GAPDH. Our results indicate that cytoglobin in cells exists primarily as oxyferrous cytoglobin (CygbFe2+-O2) with its cysteine residues in the reduced form. We found that the specific substitution of one of two cysteine residues on cytoglobin (C83A) inhibited the reductive activity of cytoglobin on Hyper7 and GAPDH. Carotid arteries from cytoglobin knockout mice were more sensitive to glycolytic inhibition by hydrogen peroxide than arteries from wildtype mice. Together, these results support a role for cytoglobin in regulating intracellular redox signals associated with hydrogen peroxide through oxidation of its cysteine residues, independent of hydrogen peroxide reaction at its heme center.
Rescue of blood coagulation Factor VIII exon-16 mis-splicing by antisense oligonucleotides
Authors: Tse Victor , Chacaltana Guillermo , Gutierrez Martin , Forino Nicholas M. , Jimenez Arcelia G. , Tao Hanzhang , Do Phong H. , Oh Catherine , Chary Priyanka , Quesada Isabel , Hamrick Antonia , Lee Sophie , Stone Michael D. , Sanford Jeremy R.
Published: April 2023

The humanFactor VIII(F8) protein is essential for the blood coagulation cascade and specificF8mutations cause the rare bleeding disorder Hemophilia A (HA). Here, we investigated the impact of HA-causing single-nucleotide mutations onF8pre-mRNA splicing. We found that 14/97 (∼14.4%) coding sequence mutations tested in our study induced exon skipping. Splicing patterns of 4/11 (∼36.4%)F8exons tested were especially sensitive to the presence of common disease-causing mutations. RNA-chemical probing analyses revealed a three-way junction structure at the 3′ end of intron 15 (TWJ-3-15). TWJ-3-15 sequesters the polypyrimidine tract, a key determinant of 3′ splice site strength. Using exon-16 of theF8gene as a model, we designed specific antisense oligonucleotides (ASOs) that target TWJ-3-15 and identified three that promote the splicing ofF8exon-16. Interaction of TWJ-3-15 with ASOs increases accessibility of the polypyrimidine tract and inhibits the binding of hnRNPA1-dependent splicing silencing factors. Moreover, ASOs targeting TWJ-3-15 rescue diverse splicing-sensitive HA-causing mutations, most of which are distal to the 3’ splice site being impacted. The TWJ-3-15 structure and its effect on mRNA splicing provide a model for HA etiology in patients harboring specificF8mutations and provide a framework for precision RNA-based HA therapies.
Quorum-sensing gene regulates hormetic effects induced by sulfonamides in Comamonadaceae
Authors: Lin Hui , Ning Xue , Wang Donglin , Wang Qiaojuan , Bai Yaohui , Qu Jiuhui
Published: April 2023

Hormesis is a toxicological phenomenon whereby exposure to low-dose stress results in stimulation of various biological endpoints. Among these, the induction of cell proliferation by antibiotics is critical, but the underlying molecular mechanisms remain poorly understood. Here, we showed that sulfonyl-containing chemicals (e.g., sulfonamides) can induce cell-proliferation hormesis ofComamonas testosteroni. Investigation of the hormesis mechanism revealed that low-dose sulfonamides bind to theLuxR-type quorum sensing protein LuxR solo, thereby triggering the transcription of 3-ketoacyl-CoA thiolase, a key enzyme of the fatty acid β-oxidation. This provides additional ATP, NADPH, and acetyl-CoA for purine and pyrimidine biosynthesis, allowing cells to synthesize sufficient nucleotides to support rapid cell growth. Our work reports on a previously unknown mechanism for the hormetic effect and highlights its generality in the Comamonadaceae family.
Discovering motifs and genomic patterns with SMT: a high-performance data structure for counting kmers
Authors: Caldonazzo Garbelini Jader M. , Sanches Danilo Sipoli , Pozo Aurora Trinidad Ramirez
Published: April 2023

Motivation

The search for conserved motifs in DNA sequences is an important problem in bioinformatics. The growing availability of large-scale genomic data poses significant challenges for computational biology, particularly in terms of efficiency in analysis, kmer identification, and noise presence. The detection of conserved motifs and patterns in DNA sequences is crucial for understanding gene functions and regulations. Therefore, it is essential to develop a data structure that can handle these large volumes of information and provide accurate and fast results.

Results

We present SMT, an innovative tool designed to efficiently store and count kmers, optimizing memory usage and computation time. The application of SMT has also proven effective in discovering motifs in noisy datasets, allowing the identification of conserved regions in sequences. Furthermore, SMT enables exact searches in constant time and recovers the most abundant k-mers, as well as performs approximate searches in linear time to find fragments with up todmutations. This approach facilitates large-scale data analysis and provides important insights into the conserved properties of biological sequences. The application of SMT in motif discovery demonstrates its potential to drive research in bioinformatics and genomics. Supplementary data and results are available to provide additional information and support the conclusions presented in this work.

Availability and implementation

The source code of the presented method is publicly available athttps://github.com/jadermcg/SMT.
3D genome organization drives gene expression in trypanosomes
Authors: Díaz-Viraqué Florencia , Chiribao María Laura , Libisch Gabriela , Robello Carlos
Published: April 2023

In trypanosomes —eukaryotic unicellular pathogens that cause disabling human and animal diseases— very few transcriptional regulatory elements have been described and it is largely accepted that they regulate gene expression mainly post-transcriptionally. In this regard, the role of the spatial organization of the genome on gene expression and vice versa remains practically unexplored. The genome of these parasites is partitioned into core (highly conserved syntenic) and species-specific disruptive regions (synteny disruption), containing multigene families encoding for surface glycoproteins. By mapping genome-wide chromatin interactions we demonstrate that these regions constitute 3D compartments (C and D). These chromatin compartments present significant differences in DNA methylation, nucleosome positioning and chromatin interactions, affecting genome expression dynamics. We show that the genome is organized into chromatin folding domains and transcription is dramatically determined by the local chromatin structure. Our results support a model in which epigenetic mechanisms dramatically impact gene expression in these eukaryotic pathogens.
The moderating role of narcissism in predicting the behavior intention of the Palestinian university students towards adopting the new Facebook currency
Authors: Baidoun Samir Daoud , Salem Mohammed Zedan , Wagner Ralf
Published: April 2023

Purpose

This paper aims to identify the factors affecting university students’ behavioral intentions towards adopting the new Facebook currency while controlling for the direct and the moderating impacts of narcissism.

Design/methodology/approach

A self-administered questionnaire of 344 respondents from six major Palestinian universities was analyzed. The structural model is fitted for assessing the hypothesized relations.

Findings

Findings indicate that the effect of Facebook advertisement in predicting the behavioral intentions to adopt the new Facebook currency relies on: privacy, security, the expectations in the new Facebook currency and knowledge about the targeting options within the Facebook platform. Moreover, the moderating role of narcissism supported the relationships between perceived privacy, the expectations in the new Facebook currency and knowledge about the targeting options within the Facebook platform but did not support the moderating role of narcissism in the relationships between perceived security and the behavioral intentions.

Research limitations/implications

In this study, only the Facebook platform and the behavioral intention were investigated with data collected from Palestinian University students through self-reported cross-sectional survey.

Practical implications

This study adds insight on the moderating role of narcissism in predicting the behavior intentions towards adopting the new Facebook currency (Diem) which has a substantial potential to threaten all other crypto currencies and the other alternatives. Therefore, managers should consider altering or adapting their Facebook advertising tactics accordingly.

Originality/value

This study is the first to contribute through empirical evidence from a developing country to theory building the results of clarifying the propensity to adopt the new Facebook currency, outlining the consumers’ reaction to social media advertising and its influential factors and providing evidence proving relevance of narcissism for non-Western users.

Peer review

The peer review history for this article is available at:https://publons.com/publon/10.1108/OIR-12-2021-0666
Racial attitudes and belief in redeemability: Most Whites believe justice‐involved Black people can change
Authors: Butler Leah C. , Cullen Francis T. , Burton Velmer S.
Published: April 2023

Public belief in redeemability reduces punitiveness and increases support for policy measures such as rehabilitation, expungement, and housing and employment opportunities. Although racial attitudes are known to influence a wide range of criminal justice policy opinions, their effects on beliefs about redeemability and condemnation have not been fully explored. Using data from a 2019 YouGov survey of a national sample of White U.S. adults (N = 766), the current study estimates the effects of three distinct racial attitudes—racial resentment, racial sympathy, and White nationalism—on three measures of belief in redeemability: 1) a race‐neutral measure, 2) a measure of belief in redeemability of Black offenders, and 3) a measure of condemnation of Black offenders. The results indicate that belief in redeemability is high—for offenders in general and for Black offenders. These findings are supported by a second 2022 YouGov survey of White U.S. adults (N = 1,505). Racial sympathy and White nationalism have significant effects across all three outcomes, with the positive effect of White nationalism on condemnation of Black offenders being the largest across the three models. These findings suggest that although most Whites agree that formerly incarcerated people are redeemable, racial attitudes influence these beliefs, especially for Black offenders.
Monitoring temporal and spatial trends of illegal and legal fishing in marine conservation areas across Canada's three oceans
Authors: Iacarella Josephine C. , Burke Lily , Clyde Georgia , Wicks Adam , Clavelle Tyler , Dunham Anya , Rubidge Emily , Woods Paul
Published: April 2023

Expansion of marine conservation areas (CA) necessitates resource‐efficient and achievable strategies for monitoring and evaluation of ongoing fishing activity at national levels. To demonstrate and explore such a strategy, we conducted the first extensive analysis of fishing activity within Canada's static, geographically defined marine CAs with fishing regulations (n = 264 areas). We used 8 years of Automatic Identification System data to estimate fishing effort across three oceans and conducted temporal and spatial comparisons specific to each CA's regulations and enactment date. We addressed questions on CA effectiveness, fishing displacement, fishing the line behavior, and relationships between fishing activity and spatial CA attributes. We estimated 22,000 h of fishing activity within CAs after enactments, 22% of which was identified as illegal. CA effectiveness appeared to be lowest for Atlantic CAs based on illegal fishing effort density within CAs. Fishing displacement and fishing the line was generally not apparent as buffer areas around CAs tended to already have higher fishing effort prior to enactments. CA effectiveness and responses to CAs varied considerably, as was visualized using timeseries plots and maps developed for each CA. Our evaluation of a nation's full suite of CAs provides managers with a foundation and approach for continued monitoring and reporting.
Glycosylation increases active site rigidity leading to improved enzyme stability and turnover
Authors: Ramakrishnan Krithika , Johnson Rachel L. , Winter Samuel D. , Worthy Harley L. , Thomas Christopher , Humer Diana C. , Spadiut Oliver , Hindson Sarah H. , Wells Stephen , Barratt Andrew H. , Menzies Georgina E. , Pudney Christopher R. , Jones D. Dafydd
Published: April 2023

Glycosylation is the most prevalent protein post‐translational modification, with a quarter of glycosylated proteins having enzymatic properties. Yet, the full impact of glycosylation on the protein structure–function relationship, especially in enzymes, is still limited. Here, we show that glycosylation rigidifies the important commercial enzyme horseradish peroxidase (HRP), which in turn increases its turnover and stability. Circular dichroism spectroscopy revealed that glycosylation increased holo‐HRP's thermal stability and promoted significant helical structure in the absence of haem (apo‐HRP). Glycosylation also resulted in a 10‐fold increase in enzymatic turnover towards o‐phenylenediamine dihydrochloride when compared to its nonglycosylated form. Utilising a naturally occurring site‐specific probe of active site flexibility (Trp117) in combination with red‐edge excitation shift fluorescence spectroscopy, we found that glycosylation significantly rigidified the enzyme. In silico simulations confirmed that glycosylation largely decreased protein backbone flexibility, especially in regions close to the active site and the substrate access channel. Thus, our data show that glycosylation does not just have a passive effect on HRP stability but can exert long‐range effects that mediate the ‘native’ enzyme's activity and stability through changes in inherent dynamics.
Four Natural Chromite Reference Materials for the Determination of the First‐Row Transition Elements and Gallium by LA‐ICP‐MS
Authors: Wang Jing , Su Ben‐Xun , Gao Bing‐Yu , Li Wen‐Jun , Cui Meng‐Meng , Liu Xia , Luo Yang , Pan Qi‐Qi , Jia Li‐Hui , Su Li , Chen Kai‐Yun
Published: April 2023

Four natural chromite samples (LBS13‐04, LBS13‐06 and LBS13‐13) from the Luobusa ophiolite (China) and 16SW2‐6 from the Stillwater Complex (USA) were developed as reference materials for in situ element microanalysis. Approximately 8 g of chromite fragments with grain sizes of 0.5–1.5 mm from each chromite sample were separated under a binocular microscope and analysed by EPMA, XRF, LA‐ICP‐MS and solution nebulisation ICP‐MS techniques for major and trace elements at six laboratories. The results show that the four chromite samples are homogeneous with respect to MgO, Al2O3, Cr2O3, FeO, Sc, Ti, V, Mn, Co, Ni, Zn and Ga. These samples are thus suitable to be used as reference materials for in situ microanalysis.
Fetal cardiac interventions: First‐year experience of a tertiary referral center in Turkey
Authors: Deren Ozgur , Aykan Hayrettin Hakan , Ozyuncu Ozgur , Aypar Ebru , Cagan Murat , Ozen Ozge , Karagoz Tevfik
Published: April 2023

Aim

To present the first‐year experience of fetal cardiac interventions (FCIs) in a tertiary referral hospital and to evaluate the outcomes.

Methods

This retrospective study consisted of four pregnant women who underwent fetal pulmonary or aortic balloon valvuloplasty between November 2020 and June 2021. The procedures were performed with a percutaneous cardiac puncture under the ultrasonography guidance. Gestational age at intervention, procedural success, complications, and perinatal outcomes were evaluated. Procedural complications defined as fetal bradyarrhythmia requiring treatment, pericardial effusion requiring drainage, balloon rupture, and fetal death. The procedure was considered technically successful if the valve was dilated with a balloon catheter. Ultimately successful procedure was defined as the discharge of infants alive with biventricular circulation.

Results

A total of 5 FCIs attempted between 26 + 3 and 28 + 2 gestational weeks. While the procedure was technically successful in 2 cases with pulmonary stenosis, both attempts were unsuccessful in the fetus with pulmonary atresia. Although the procedure was technically successful in the patient with critical aortic stenosis, it ultimately failed. No fetal death occurred in our series and there were no procedure‐related significant maternal complications. However, three interventions were complicated by fetal bradycardia and pericardial effusion necessitating treatment, and balloon rupture cropped up in one case.

Conclusion

FCIs may lead to improving the likelihood of a biventricular outcome for selected fetuses. Careful selection of patients and centralization of experience are essential for obtaining favorable outcomes. Operators should be aware of procedural complications. Improved procedural techniques with a lower complication rate will be achieved through advanced medical technology and special balloon catheters.
More complex than you think: Taxonomic and temporal patterns of plant–pollinator networks of caraway (Carum carvi L.)
Authors: Kilian Isabel C. , Swenson Stephanie J. , Mengual Ximo , Gemeinholzer Birgit , Hamm Andrée , Wägele J. Wolfgang , Peters Ralph S.
Published: April 2023

Caraway (Carum carvi L.) is a crop species that is gaining in importance in Europe, especially as a condiment and medicinal plant. Here, we present the plant–pollinator network of caraway in a central European agricultural landscape, focusing on two diverse potential pollinator taxa, Diptera: Brachycera (= true flies) and Hymenoptera (sawflies, bees, and wasps). We specifically studied qualitative differences in interactions between the two insect taxa as well as the intraday and intraseasonal variability of the network. Insect and pollen plant species determination was done via morphological identification and DNA (meta)barcoding. In total, 121 species representing 33 families of Hymenoptera and Brachycera were found to carry caraway pollen. These taxa included many nonhoneybee and nonhoverfly species, showing a wide taxonomic breadth of potential pollinators and a higher network complexity than previously anticipated. There are distinct qualitative differences between Brachycera and Hymenoptera networks, suggesting complementary roles of both taxa in the pollination of native and crop plants. Strong intraday differences in potential pollinator diversity make it necessary to collect insects and pollen at different times of the day to compile complete plant–pollinator networks. Intraseasonal analyses of the plant–pollinator network of caraway show the potential of caraway as an important food source for insect species with an activity peak in late summer.
Occurrence and field evaluation of sugarcane genotypes for resistance to pokkah boeng disease in China
Authors: Xu Yuzhi , Wei Yibin , Jiang Chunxiu , Pan Kaiyuan , Wang Maoyao , Deng Zuhu , Zhang Muqing
Published: April 2023

Sugarcane pokkah boeng disease (PBD), caused by aFusariumspecies complex, is one of the most critical diseases causing a significant economic loss of sugarcane production in China. It is important to standardize the evaluation method for screening sugarcane cultivars for resistance to PBD. Field trials were conducted to investigate the occurrence of sugarcane PBD in different months and growth stages from 2018 to 2019 and to evaluate the PBD resistance of 169 sugarcane genotypes from 2019 to 2022. PBD mainly occurred in the early and middle elongation stages at a plant height of approximately 1–2 m. The most severe and common incidence of PBD was from June to August. Variance analysis showed genotype, year and the interaction of genotype and year had a significant effect on disease incidence and disease index. The 169 sugarcane genotypes were divided into five categories by cluster analysis and discriminant analysis, of which 33 were rated as highly resistant, 37 as resistant, 38 as moderately susceptible, 26 as susceptible and 35 as highly susceptible. These findings enhance our understanding of sugarcane resistance to PBD and lay the foundation for breeding new resistant varieties.
DIGITAL THEOLOGY AND A POTENTIAL THEOLOGICAL APPROACH TO A METAPHYSICS OF INFORMATION
Authors: Phillips Peter M.
Published: April 2023

In this article, I offer a background to digital theology and its methodology, exploring especially aspects of transhumanism and metaphysical enquiry. The article moves on to engage with several articles given at the Science and Religion Forum at Birmingham in 2022, especially the Gowland Lecture given by Professor Niels Gregersen and the Peacocke Lecture by Andrew Jackson. Both offer a metaphysical approach to information linked closely to the concept of Logos drawn from the Prologue of John—Jackson focusing on Maximus the Confessor's exploration of phylogeneticlogoi; Gregersen on a further development of “Deep Incarnation” through the title “God with Clay” drawn from Bonaventure. The article extends this engagement with John by querying the model of incarnation in “deep incarnation” but building on the Logos/logoi to set out some initial building blocks for an alternative metaphysics of information.
RESPONSIVE BODIES: ROBOTS, AI, AND THE QUESTION OF HUMAN DISTINCTIVENESS
Authors: Balle Simon , Nissen Ulrik
Published: April 2023

In this article, we argue two points in relation to the challenge to human distinctiveness emerging as artificial intelligence systems and humanlike robots simulate various human capabilities. First, that, in the context of theological anthropology, it is advisable to respond to this challenge by turning toward the human body. Second, following this point, we propose the responsive body hypothesis, suggesting that what makes us distinct from androids are capacities that rise from and depend on our responsive bodies.
The Impact of Media Reputation, Customer Satisfaction, Digital Banking, and Risk Management Moderation on ROA
Authors: Mappanyuki Ratna , Anfusina Min Sururi , Alam Syamsu
Published: April 2023

This research aims to analyze the effect of media reputation, customer satisfaction, and digital banking on the financial performance of Rural Banks (BPR) with risk management moderation. The population of BPRs from 2019 to 2020 was 1,403 by using the purposive sampling method which obtained 29 BPR samples. This study uses the MRA analysis model and Eviews. The empirical findings conclude that customer satisfaction has a significant positive effect on financial performance, while media reputation and digital banking have no impact on financial performance. The results of moderated regression analysis concluded that risk management did not moderate the relationship of the three variables toward financial performance. This study is expected to provide input for BPR management in Indonesia to be more intense in reanalyzing all aspects that can support the improvement of BPR bank performance to modernize the service system, be more attentive to the internal management to avoid the internal fraud cases, and maintain the good image to prevent the possible bad news that can damage the bank's reputation.
Multiple CD59 Polymorphisms in Chinese Patients with Mycobacterium tuberculosis Infection
Authors: Tang Jie , Zhao Zhenzhen , Zhou Juan , Jiao Lin , Zhou Wenjing , Ying Binwu , Yang Yuwei
Published: April 2023

Background and Objective. Tuberculosis (TB) is a major threat to human health, especially in developing countries. Its susceptibility and progression depend on interactions between mycobacterium tuberculosis, host immune system, and genetic and environmental factors. Up to now, many studies have presented the association between TB susceptibility and host genetic polymorphisms, but never regarding CD59 gene, which is an essential complement regulator. This study investigated the relationship between multiple CD59 single nucleotide polymorphisms (SNPs) and susceptibility to TB among Chinese patients. Methods. A case–control study was conducted to investigate the SNPs at CD59 rs1047581, rs7046, rs2231460, rs184251026, rs41275164, rs831633, rs704700, rs41275166, and rs10768024 by sequence-specific primer-polymerase chain reaction (SSP-PCR) in 900 tuberculosis patients and 1,534 controls. Results. The minor allele frequencies at rs2231460, rs184251026, rs41275164, and rs41275166 were extremely low both in the Cases (0.00%–0.61%) and in the Controls (0.07%–0.43%), comparatively at rs1047581, rs7046, rs831633, rs704700, and rs10768024 were notably higher both in the Cases (8.23%–48.39%) and in the Controls (8.57%–47.16%). Among the nine SNPs, only homozygous CC genotype at rs10768024 showed a significant protective effect against TB than homozygous TT genotype (OR(95% CI) = 0.59(0.38, 0.91), χ2 = 5.779,

P
=
0.016

), and homozygous TT and heterozygous CT genotypes showed a significant risk of TB infection in the recessive model (OR(95% CI) = 1.68(1.10, 2.56), χ2 = 5.769,

P
=
0.016

). Further analysis verified that rs10768024 CC genotype independently related to TB susceptibility (OR(95% CI) = 0.60(0.39, 0.91), Wald χ2 = 5.664,

P
=
0.017

) in multivariate logistic regression analysis, and its genetic mutation was independent of the other SNPs (r2 = 0.00–0.20) in haplotype analysis. Conclusions. The first investigation of the CD59 gene and susceptibility to TB suggests a significant risk with homozygous TT and heterozygous CT genotypes at rs10768024 loci. The homozygous CC mutation at rs10768024 loci showed a significant protection against TB susceptibility.
Supplementary Figure 1 from Characterization of an Akt Kinase Inhibitor with Potent Pharmacodynamic and Antitumor Activity
Authors: Rhodes Nelson , Heerding Dirk A. , Duckett Derek R. , Eberwein Derek J. , Knick Victoria B. , Lansing Timothy J. , McConnell Randy T. , Gilmer Tona M. , Zhang Shu-Yun , Robell Kimberly , Kahana Jason A. , Geske Robert S. , Kleymenova Elena V. , Choudhry Anthony E. , Lai Zhihong , Leber Jack D. , Minthorn Elisabeth A. , Strum Susan L. , Wood Edgar R. , Huang Pearl S. , Copeland Robert A. , Kumar Rakesh
Published: April 2023

Supplementary Figure 1 from Characterization of an Akt Kinase Inhibitor with Potent Pharmacodynamic and Antitumor Activity
Supplementary Figure 2 from Integrative Microarray Analysis of Pathways Dysregulated in Metastatic Prostate Cancer
Authors: Setlur Sunita R. , Royce Thomas E. , Sboner Andrea , Mosquera Juan-Miguel , Demichelis Francesca , Hofer Matthias D. , Mertz Kirsten D. , Gerstein Mark , Rubin Mark A.
Published: April 2023

Supplementary Figure 2 from Integrative Microarray Analysis of Pathways Dysregulated in Metastatic Prostate Cancer
Supplementary Figure 1 from Integrative Microarray Analysis of Pathways Dysregulated in Metastatic Prostate Cancer
Authors: Setlur Sunita R. , Royce Thomas E. , Sboner Andrea , Mosquera Juan-Miguel , Demichelis Francesca , Hofer Matthias D. , Mertz Kirsten D. , Gerstein Mark , Rubin Mark A.
Published: April 2023

Supplementary Figure 1 from Integrative Microarray Analysis of Pathways Dysregulated in Metastatic Prostate Cancer
Supplementary Table 1 from Identification of Genes Required for Protection from Doxorubicin by a Genome-Wide Screen in Saccharomyces cerevisiae
Authors: Xia Ling , Jaafar Lahcen , Cashikar Anil , Flores-Rozas Hernan
Published: April 2023

Supplementary Table 1 from Identification of Genes Required for Protection from Doxorubicin by a Genome-Wide Screen in Saccharomyces cerevisiae
Supplementary Figure 2 from Identification of Genes Required for Protection from Doxorubicin by a Genome-Wide Screen in Saccharomyces cerevisiae
Authors: Xia Ling , Jaafar Lahcen , Cashikar Anil , Flores-Rozas Hernan
Published: April 2023

Supplementary Figure 2 from Identification of Genes Required for Protection from Doxorubicin by a Genome-Wide Screen in Saccharomyces cerevisiae
Supplementary Figure 1 from Identification of Genes Required for Protection from Doxorubicin by a Genome-Wide Screen in Saccharomyces cerevisiae
Authors: Xia Ling , Jaafar Lahcen , Cashikar Anil , Flores-Rozas Hernan
Published: April 2023

Supplementary Figure 1 from Identification of Genes Required for Protection from Doxorubicin by a Genome-Wide Screen in Saccharomyces cerevisiae
Supplementary Table 1 from Gene Alterations Identified by Expression Profiling in Tumor-Associated Endothelial Cells from Invasive Ovarian Carcinoma
Authors: Lu Chunhua , Bonome Tomas , Li Yang , Kamat Aparna A. , Han Liz Y. , Schmandt Rosemarie , Coleman Robert L. , Gershenson David M. , Jaffe Robert B. , Birrer Michael J. , Sood Anil K.
Published: April 2023

Supplementary Table 1 from Gene Alterations Identified by Expression Profiling in Tumor-Associated Endothelial Cells from Invasive Ovarian Carcinoma
Supplementary Information and Tables 1-5 from Association Study of 69 Genes in the Ret Pathway Identifies Low-penetrance Loci in Sporadic Medullary Thyroid Carcinoma
Authors: Ruiz-Llorente Sergio , Montero-Conde Cristina , Milne Roger L. , Moya Christian M. , Cebrián Arancha , Letón Rocío , Cascón Alberto , Mercadillo Fátima , Landa Iñigo , Borrego Salud , de Nanclares Guiomar Pérez , Álvarez-Escolá Cristina , Díaz-Pérez José Ángel , Carracedo Ángel , Urioste Miguel , González-Neira Anna , Benítez Javier , Santisteban Pilar , Dopazo Joaquín , Ponder Bruce A. , Robledo Mercedes
Published: April 2023

Supplementary Information and Tables 1-5 from Association Study of 69 Genes in the Ret Pathway Identifies Low-penetrance Loci in Sporadic Medullary Thyroid Carcinoma
Supplementary Figure 2 from Cyclooxygenase-2 Transcription Is Regulated by Human Papillomavirus 16 E6 and E7 Oncoproteins: Evidence of a Corepressor/Coactivator Exchange
Authors: Subbaramaiah Kotha , Dannenberg Andrew J.
Published: April 2023

Supplementary Figure 2 from Cyclooxygenase-2 Transcription Is Regulated by Human Papillomavirus 16 E6 and E7 Oncoproteins: Evidence of a Corepressor/Coactivator Exchange
Supplementary Legends for Figures 1-3, Videos 1-2 from R-Cadherin Expression Inhibits Myogenesis and Induces Myoblast Transformation via Rac1 GTPase
Authors: Kucharczak Jérôme , Charrasse Sophie , Comunale Franck , Zappulla Jacques , Robert Bruno , Teulon-Navarro Isabelle , Pèlegrin André , Gauthier-Rouvière Cécile
Published: April 2023

Supplementary Legends for Figures 1-3, Videos 1-2 from R-Cadherin Expression Inhibits Myogenesis and Induces Myoblast Transformation via Rac1 GTPase
Supplementary Figure 8 from Different S/M Checkpoint Responses of Tumor and Non–Tumor Cell Lines to DNA Replication Inhibition
Authors: Rodríguez-Bravo Verónica , Guaita-Esteruelas Sandra , Salvador Noelia , Bachs Oriol , Agell Neus
Published: April 2023

Supplementary Figure 8 from Different S/M Checkpoint Responses of Tumor and Non–Tumor Cell Lines to DNA Replication Inhibition
Supplementary Figure 5 from Suppression of Proximal T Cell Receptor Signaling and Lytic Function in CD8+ Tumor-Infiltrating T Cells
Authors: Monu Ngozi , Frey Alan B.
Published: April 2023

Supplementary Figure 5 from Suppression of Proximal T Cell Receptor Signaling and Lytic Function in CD8+ Tumor-Infiltrating T Cells
Supplementary Figure 1 from Suppression of Proximal T Cell Receptor Signaling and Lytic Function in CD8+ Tumor-Infiltrating T Cells
Authors: Monu Ngozi , Frey Alan B.
Published: April 2023

Supplementary Figure 1 from Suppression of Proximal T Cell Receptor Signaling and Lytic Function in CD8+ Tumor-Infiltrating T Cells
Supplementary Figure 1 from Detection of Early Prostate Cancer Using a Hepsin-Targeted Imaging Agent
Authors: Kelly Kimberly A. , Setlur Sunita R. , Ross Robert , Anbazhagan Rajesh , Waterman Peter , Rubin Mark A. , Weissleder Ralph
Published: April 2023

Supplementary Figure 1 from Detection of Early Prostate Cancer Using a Hepsin-Targeted Imaging Agent
Supplementary Table 1 from The Human let-7a-3 Locus Contains an Epigenetically Regulated MicroRNA Gene with Oncogenic Function
Authors: Brueckner Bodo , Stresemann Carlo , Kuner Ruprecht , Mund Cora , Musch Tanja , Meister Michael , Sültmann Holger , Lyko Frank
Published: April 2023

Supplementary Table 1 from The Human let-7a-3 Locus Contains an Epigenetically Regulated MicroRNA Gene with Oncogenic Function
Supplementary Table 3 from Cancer Cell Lines as Genetic Models of Their Parent Histology: Analyses Based on Array Comparative Genomic Hybridization
Authors: Greshock Joel , Nathanson Katherine , Martin Anne-Marie , Zhang Lin , Coukos George , Weber Barbara L. , Zaks Tal Z.
Published: April 2023

Supplementary Table 3 from Cancer Cell Lines as Genetic Models of Their Parent Histology: Analyses Based on Array Comparative Genomic Hybridization
Supplementary Figure 1 from Histone Deacetylase 2 Modulates p53 Transcriptional Activities through Regulation of p53-DNA Binding Activity
Authors: Harms Kelly Lynn , Chen Xinbin
Published: April 2023

Supplementary Figure 1 from Histone Deacetylase 2 Modulates p53 Transcriptional Activities through Regulation of p53-DNA Binding Activity
Supplementary Table 2 from Cancer Cell Lines as Genetic Models of Their Parent Histology: Analyses Based on Array Comparative Genomic Hybridization
Authors: Greshock Joel , Nathanson Katherine , Martin Anne-Marie , Zhang Lin , Coukos George , Weber Barbara L. , Zaks Tal Z.
Published: April 2023

Supplementary Table 2 from Cancer Cell Lines as Genetic Models of Their Parent Histology: Analyses Based on Array Comparative Genomic Hybridization
Supplementary Tables 1-2, Figure 1 from Mechanism of All-Trans Retinoic Acid Effect on Tumor-Associated Myeloid-Derived Suppressor Cells
Authors: Nefedova Yulia , Fishman Mayer , Sherman Simon , Wang Xingyu , Beg Amer A. , Gabrilovich Dmitry I.
Published: April 2023

Supplementary Tables 1-2, Figure 1 from Mechanism of All-Trans Retinoic Acid Effect on Tumor-Associated Myeloid-Derived Suppressor Cells
Supplementary Figure 3 from Cancer Cell Death Enhances the Penetration and Efficacy of Oncolytic Herpes Simplex Virus in Tumors
Authors: Nagano Satoshi , Perentes Jean Yannis , Jain Rakesh K. , Boucher Yves
Published: April 2023

Supplementary Figure 3 from Cancer Cell Death Enhances the Penetration and Efficacy of Oncolytic Herpes Simplex Virus in Tumors
Supplementary Tables 1-2 from The Original Pathologische Anatomie Leiden-Endothelium Monoclonal Antibody Recognizes a Vascular Endothelial Growth Factor–Binding Site within Neuropilin-1
Authors: Jaalouk Diana E. , Ozawa Michael G. , Sun Jessica , Lahdenranta Johanna , Schlingemann Reinier O. , Pasqualini Renata , Arap Wadih
Published: April 2023

Supplementary Tables 1-2 from The Original Pathologische Anatomie Leiden-Endothelium Monoclonal Antibody Recognizes a Vascular Endothelial Growth Factor–Binding Site within Neuropilin-1
Supplementary Figures 1-2 from Loss of MLL PHD Finger 3 Is Necessary for MLL-ENL–Induced Hematopoietic Stem Cell Immortalization
Authors: Chen Jing , Santillan Donna A. , Koonce Mark , Wei Wei , Luo Roger , Thirman Michael J. , Zeleznik-Le Nancy J. , Diaz Manuel O.
Published: April 2023

Supplementary Figures 1-2 from Loss of MLL PHD Finger 3 Is Necessary for MLL-ENL–Induced Hematopoietic Stem Cell Immortalization
Supplementary Methods from High SEPT9_v1 Expression in Human Breast Cancer Cells Is Associated with Oncogenic Phenotypes
Authors: Gonzalez Maria E. , Peterson Esther A. , Privette Lisa M. , Loffreda-Wren Janice L. , Kalikin Linda M. , Petty Elizabeth M.
Published: April 2023

Supplementary Methods from High SEPT9_v1 Expression in Human Breast Cancer Cells Is Associated with Oncogenic Phenotypes
Supplementary Figure 1 from The Nucleolin Targeting Aptamer AS1411 Destabilizes Bcl-2 Messenger RNA in Human Breast Cancer Cells
Authors: Soundararajan Sridharan , Chen Weiwei , Spicer Eleanor K. , Courtenay-Luck Nigel , Fernandes Daniel J.
Published: April 2023

Supplementary Figure 1 from The Nucleolin Targeting Aptamer AS1411 Destabilizes Bcl-2 Messenger RNA in Human Breast Cancer Cells
Supplementary Figure Legends 1-6 from CDC25B Involvement in the Centrosome Duplication Cycle and in Microtubule Nucleation
Authors: Boutros Rose , Lobjois Valérie , Ducommun Bernard
Published: April 2023

Supplementary Figure Legends 1-6 from CDC25B Involvement in the Centrosome Duplication Cycle and in Microtubule Nucleation
Supplementary Figure 4 from CDC25B Involvement in the Centrosome Duplication Cycle and in Microtubule Nucleation
Authors: Boutros Rose , Lobjois Valérie , Ducommun Bernard
Published: April 2023

Supplementary Figure 4 from CDC25B Involvement in the Centrosome Duplication Cycle and in Microtubule Nucleation