bluuebunny/crossref_metadata_2025 huggingface dataset:
744. LEFT THORACOABDOMINAL ESOPHAGECTOMY: ITS VALIDITY IN MODERN ESOPHAGEAL SURGERY
Authors: Dellaportas Dionysios , Margaris Ioannis , Latsonas Panagiotis , Pikouli Anastasia , Vlachos Iakovos , Papaconstantinou Dimitrios , Pararas Nikolaos , Nastos Constantinos , Myoteri Despoina , Pikoulis Emmanuel
Published: September 2024

Background

The optimal surgical approach for the treatment of esophageal and gastroesophageal junction tumors remains elusive. An open left thoracoabdominal approach may prove particularly useful in certain clinical scenarios, mainly because of its versatility and excellent exposure provided. The aim of this study is to present our experience and evaluate the early outcomes related to an open left thoracoabdominal esophagectomy (LTE).

Methods

A retrospective cohort study of prospectively collected data was conducted from two institutions performing esophageal surgery. Patients with distal esophageal or junctional tumors who underwent an open LTE between November 2018 and December 2023 were included. Primary outcomes were 30-day and in-hospital mortality, postoperative complications, and surrogates of oncological efficacy, including resection margin status and lymph node yield. Complications were recorded using the definitions provided by the Esophagectomy Complications Consensus Group and scored according to the Clavien-Dindo classification. Circumferential resection margin status was based on the definition used by the College of American Pathologists.

Results

During the study period 22 patients underwent an open LTE with a standard two-field lymphadenectomy. Eight patients (36%) suffered from postoperative complications, with pulmonary complications being the most common (7 patients; 32%). Severe complications (Clavien-Dindo ≥3) occurred in 3 patients. One patient experienced a clinical anastomotic leak. None of the patients required a reoperation or a step-up to a higher level of care. The 30-day and in-hospital mortality were zero. On histological examination, 6 patients (27%) had tumor cells at the surgical margin, most commonly the circumferential resection margin. A mean number of 27 lymph nodes were retrieved.

Conclusion

This small series demonstrates the applicability of an open left thoracoabdominal approach in everyday surgical practice for the treatment of distal esophageal and gastroesophageal junction tumors. Our results imply that open LTE can be implemented in select patients with acceptable morbidity and oncological efficacy.
769. CLINICAL EVALUATION OF CRYOBALLOON ABLATION FOR BARRETTS OESOPHAGUS: A UK FIRST MULTI-CENTRE EXPERIENCE
Authors: Aslam Nasar , Gernter Julain , Duku Margaret , Britton James , Ang Yeng , Sehgal Vinay
Published: September 2024

Introduction

The Cryoballoon Focal Ablation System (CbFAS) (Pentax Medical Inc) is an innovative device designed for the endoscopic ablation of Barrett's-related neoplasia. This system has been shown to provide advantages such as reduced stricture rates and improved patient tolerance compared to the conventional standard of care, radiofrequency ablation (RFA).

We report early outcomes from a prospective UK first multi-centre registry of consecutive patients who underwent Cryoablation for the endoscopic eradication (EET) of Barrett’s-related neoplasia.

Methods

Patients were prospectively enrolled from two tertiary referral centres for Barrett’s Oesophagus (BE). Inclusion criteria included patients with a BE segment no longer than 5cm with histologically-proven dysplasia on biopsy or endoscopic resection specimens suitable for EET of BE after MDT review. Cryoablation sessions were conducted at 3-month intervals until endoscopic eradication was complete. The standardised protocol involved administering 8 seconds per ablation for all enrolled patients with the CbFAS. Our primary outcome was complete eradication of BE. Secondary outcomes included the number of ablations performed, adverse events, device malfunction, stricture rate, patient tolerance, and endoscopist satisfaction which was evaluated using a 10-point Likert scale (1=poor, 10=excellent).

Results

Between January 2020 and December 2023, a total of 16 patients (35 treatment sessions) were treated. The median BE length was C0M2. Complete eradication of BE was achieved in 89% (8/9) of patients who had completed treatment. No instances of device malfunction or intraoperative adverse events were recorded. A stricture rate of 12.5% (2/16) was observed, both of which were successfully managed through endoscopic dilatation. All patients were recorded as tolerating the procedure well with sedation. The median endoscopist satisfaction score was 9 (+/- 2.4) with only one recorded instance of difficulty attributed to balloon positioning.

Conclusions

These preliminary results from the UK cryoablation registry suggest that cryoablation with the CbFAS is a safe and effective therapy for BE-related dysplasia. The stricture rate appears to be comparable to that observed with RFA and similar studies. With the planned enrolment of further centres and suitable patients, this registry will further enhance our understanding of the long-term efficacy and safety profile of cryoablation using the CbFAS.
792. EVALUATION OF EXTENSIVE MYOTOMY ASSOCIATED WITH DOR-MODIFIED ANTERIOR FUNDOPLICATION (PINOTTI) FOR SHORT- AND LONG-TERM TREATMENT OF ACHALASIA: A COHORT STUDY
Authors: Lisboa Fernando , Gomes Nathan , Ferreira Luis Fernando , Diniz Gabriel , Alves Emerson , Franca Henrique
Published: September 2024

Introduction

Achalasia is the main esophageal motility disorder and has a significant negative impact on the patient's quality of life. The management of achalasia primarily aims to reduce LES pressure. Surgical treatment involves a

Heller myotomy, combined with a fundoplication to prevent reflux.

Method

Prospective cohort study with 79 patients in two hospitals, one public and one private, in the city of Natal, Brazil. To evaluate the effectiveness of the surgical technique developed with the intention of treating achalasia, a long myotomy associated with the modified Dor anterior fundoplication (Pinotti). All patients were evaluated postoperatively as follows: 1) first week after surgery they were interviewed by the master surgeon; 2) thirty days after surgery they were interviewed regarding symptoms of achalasia in the postoperative period.

Results

Seventy nine (79) patients were submitted for a long myotomy procedure associated with modified Dor anterior fundoplication (Pinotti).

Seventy nine (79) patients were followed up. Although, seventy three (73) patients had improvement in symptoms related to achalasia and are clinically satisfied. Six (6) patients had recurrence of achalasia symptoms, such as dysphagia.

Conclusion

The long myotomy associated with the modified Dor anterior fundoplication (Pinotti) was the standard procedure in the study and obtained good results in 89,83% of the cases followed during the control of achalasia symptoms with a minimum rate of adverse effects in the postoperative period
811. POOLED 1-YEAR SAFETY AND QUALITY-OF-LIFE DATA AFTER REFLUXSTOP SURGERY AT TWO LARGE HOSPITALS IN SWITZERLAND: A RETROSPECTIVE CHART ANALYSIS
Authors: Zehetner Joerg , Fringeli Yannick , Linas Ioannis , Kröll Dino , Haltmeier Tobias , Borbély Yves
Published: September 2024

Background

The standards of care in reflux disease management are proton pump inhibitor (PPI) therapy and conventional antireflux surgery, however, both options carry the caveats of medication irresponsiveness and troublesome postoperative sequelae, respectively. More specifically, PPIs respond inadequately in up to 40% of cases and Nissen fundoplication is associated with postoperative dysphagia in a significant proportion of patients. RefluxStop is an innovative technology that was adopted at our two respective institutions following promising safety and effectiveness data from its CE mark trial. We present the pooled safety and quality-of-life data of our 1-year experience with RefluxStop surgery.

Methods

Between September 2018 and August 2023, RefluxStop surgery was performed on 121 patients as part of routine clinical care at two hospitals, both of which are high-volume centers for antireflux surgery. The procedure involves crural repair with hiatal hernia reduction, reconstruction of the flap valve and angle of His, narrow esophagogastric (90-110°) plication, and device implantation in an external pouch on the gastric fundus (Figure 1). A retrospective chart analysis was conducted to document perioperative adverse events (AEs) and quality-of-life improvements via Gastroesophageal Reflux Disease Health-Related Quality of Life (GERD-HRQL) score. Higher GERD-HRQL scores are indicative of more severe disease.

Results

Baseline patient characteristics included mean age 54.7 years, female sex (52.9%), body mass index 27.2±4.1 kg/m2, large hiatal hernia >3 cm (46.3%) of median (IQR) size 3 (2-5) cm, abnormal esophageal motility (60.3%), and Barrett’s esophagus (27.3%). At 1-year follow-up, patients experienced a median (IQR) reduction of 90.5% (81.4-100%) in total GERD-HRQL score. Intraoperative AEs included conversion to laparotomy for adhesiolysis with bleeding (n=1), bleeding short gastric arteries leading to return to the operating room the next day (n=1), and esophageal perforation repaired intraoperatively (n=1). Postoperative AEs are summarized in Table 1.

Conclusion

The results of this pooled retrospective chart analysis support the promising early data of the RefluxStop CE mark trial. Few and predictable AEs occurred during the study period while significant improvements in quality of life were observed, as evidenced by 90.5% (81.4-100%) reductions in GERD-HRQL score. Notably, this pooled cohort of patients was comprised of substantial proportions of difficult-to-treat patients (i.e., abnormal esophageal motility in 60.3%, large hiatal hernia >3 cm in 46.3%), providing evidence for wider implementation of RefluxStop surgery in real-world settings. Additional evaluation is required to further delineate the role of this promising innovative antireflux surgery.
Reproducibility of cardiac computed tomography classifications of hypoattenuated thickening and peridevice leak following left atrial appendage closure
Authors: Kramer Anders , Lo Russo Gerardo , Alarouri Hasan S , Collins Jeremy D , Møller Jensen Jesper , Nielsen-Kudsk Jens Erik , Alkhouli Mohamad , Korsholm Kasper
Published: September 2024

Aims

To assess the reproducibility of interpreting hypoattenuated thickening (HAT) and peridevice leak (PDL) using cardiac computed tomography (CT) imaging following Watchman FLX left atrial appendage closure (LAAC).

Methods and results

In this multicentre retrospective reproducibility study, 100 anonymized post-LAAC cardiac CT scans were evaluated within the same cardiac phase by an experienced and a novice rater blinded to prior evaluations. All scans were evaluated twice by each rater, assessing overall HAT and PDL categories as well as specific associated findings based on suggested algorithms for post-LAAC interpretation. Inter- and intra-rater agreement and reliability were evaluated using absolute agreement, Cohen’s kappa, and Kendall’s tau for categorical variables, and mean difference, Bland–Altman plots, limits of agreement (lower and upper), and intra-class correlation coefficients (ICCs) for continuous variables. Within overall categories of both HAT and PDL, substantial agreement (kappa ≥0.61) and reliability (Kendall’s tau-b ≥ 0.75) were observed. Specifically, identifying high-grade HAT (kappa ≥0.78) and distal patency (kappa ≥0.85) displayed the highest agreement within HAT and PDL interpretation. Meanwhile, measuring the height of the proximal screw hub cove represented the least reliable HAT assessment among both inter- and intra-rater comparisons (ICC < 0.75), while suspected leak mechanism represented the least reproducible PDL measure.

Conclusion

Despite only minimal training of one rater, overall high levels of inter- and intra-rater agreement and reliability were observed across the chosen algorithms for interpretation of HAT and PDL following Watchman FLX LAAC. The prognostic implications of the included variables are to be explored in future trials and registries.
Rest, replace, and recover: TandemHeart to transcatheter aortic valve replacement—a case report
Authors: Haq Syed H , Shah Sidra R , Eapen David , Kleman Anna , Knous Mallory , Laird Amanda , Cole William , Patel Sandeep M
Published: September 2024

Background

Severe aortic stenosis (AS) can present insidiously, with the end stages resulting in significant valvular–induced cardiomyopathy and can lead to cardiogenic shock (CS). Such cases result in a myriad of complex manifestations and are often associated with a poor prognosis. These patients require emergent cardiac evaluation and valvular intervention. Unfortunately, the immediate nature of the CS provides little time for a detailed valvular evaluation. Possible management involves use of mechanical circulatory support (MCS) prior to urgent transcatheter aortic valve replacement (TAVR).

Case summary

The patient was a 70-year-old female who developed refractory CS, and acute decompensated heart failure was complicated by AV block secondary to severe AS. Due to progressively worsening haemodynamics, the need for MCS for cardiovascular support and eventual valve replacement resulted in the decision to pursue TandemHeart® (TH; LivaNova Inc, Pittsburgh, PA, USA). We discuss the novel implementation of the TH as a means of bridging to TAVR.

Discussion

TandemHeart system provides the benefits of improving haemodynamic support in CS while allowing unencumbered access to the stenotic valve for balloon aortic valvuloplasty (BAV) or TAVR. In our evaluation, we discuss the utilization and benefits associated with TH to TAVR in allowing for cardiac rest, replacement of the valve, and recovery of left ventricular function.
The Debate on the Role of Orders in France, 1787–1789
Authors: Fitzsimmons Michael P
Published: September 2024

The attitude towards the three orders of the kingdom (the clergy, the nobility and the commoners) on the eve of the French Revolution remains little examined and has often been misunderstood. Orders became controversial in the aftermath of the meeting of the Assembly of Notables in 1787, which had been called to address a major fiscal crisis. The failure by the Notables to yield fiscal privileges gave rise to the perception that the Notables had placed their interests above the good of the state. Demand for the convocation of the Estates General grew, with the most critical issue being the form of voting. It had been done by order when the body last met in 1614, but in 1788–9 the Third Estate demanded a doubling of its representation and voting by head. The debate was intense and prolonged, but it focused only on orders as a basis for voting—there was little animus directed at orders themselves. When the Estates General opened, it immediately fell into a deadlock that went on for weeks, until clerical and noble deputies entered the National Assembly on 27 June and tacitly acceded to vote by head. Distinction of orders did not fall into desuetude, however. The Assembly continued to observe it in its seating until the meeting of the night of August 4. Although the Assembly itself abandoned distinction of orders, distinction of orders continued to exist in society. It was abolished in an almost incidental fashion in October 1789, and the abolition laid the foundation for two of the bitter divides of the Revolution.
Arthropod Phylotranscriptomics With a Special Focus on the Basal Phylogeny of the Myriapoda
Authors: Su Zhi-Hui , Sasaki Ayako , Minami Hiroaki , Ozaki Katsuhisa
Published: September 2024

Arthropoda represents the most diverse animal phylum, but clarifying the phylogenetic relationships among arthropod taxa remains challenging given the numerous arthropod lineages that diverged over a short period of time. In order to resolve the most controversial aspects of deep arthropod phylogeny, focusing on the Myriapoda, we conducted phylogenetic analyses based on ten super-matrices comprised of 751 to 1,233 orthologous genes across 64 representative arthropod species, including 28 transcriptomes that were newly generated in this study. Our findings provide unambiguous support for the monophyly of the higher arthropod taxa, Chelicerata, Mandibulata, Myriapoda, Pancrustacea, and Hexapoda, while the Crustacea are paraphyletic, with the class Remipedia supported as the lineage most closely related to hexapods. Within the Hexapoda, our results largely affirm previously proposed phylogenetic relationships among deep hexapod lineages, except that the Paraneoptera (Hemiptera, Thysanoptera, and Psocodea) was recovered as a monophyletic lineage in some analyses. The results corroborated the recently proposed phylogenetic framework of the four myriapod classes, wherein Symphyla and Pauropoda, as well as Chilopoda and Diplopoda, are each proposed to be sister taxa. The findings provide important insights into understanding the phylogeny and evolution of arthropods.
Late-Life Homelessness: A Definition to Spark Action and Change
Authors: Grenier Amanda , Sussman Tamara
Published: September 2024

Background and Objectives

Comprehensive definitions of social issues and populations can set the stage for the development of responsive policies and practices. Yet despite the rise of late-life homelessness, the phenomenon remains narrowly understood and ill-defined.

Research Design and Methods

This article and the definition that ensued are based on the reconceptualization of interview data derived from a critical ethnography conducted in Montreal, Canada, with older homeless persons (N = 40) and service providers (N = 20).

Results

Our analysis suggests that definitions of late-life homelessness must include 4 intersecting components: (1) age, eligibility, and access to services; (2) disadvantage over the life course and across time; (3) social and spatial processes of exclusion that necessitate aging in “undesirable” places; and (4) unmet needs that result from policy inaction and nonresponse.

Discussion and Implications

The new definition derived from these structural and relational components captures how the service gaps and complex needs identified in earlier works are shaped by delivery systems and practices whose effect is compounded over time. It provides an empirically grounded and conceptually solid foundation for the development of better responses to address homelessness in late life.
Do Experimental Forests and Ranges of the Southeastern United States Represent the Climate, Ecosystem Structure, and Ecosystem Functions of the Region?
Authors: Xiao Jingfeng , Sun Ge , Potter Kevin M , Boggs Johnny , Zhang Qingyuan , McNulty Steven G
Published: September 2024

There are twenty experimental forest and range sites (EFRs) across the southeastern United States that are currently maintained by the USDA Forest Service (Forest Service) to conduct forest ecosystem research for addressing ecosystem management challenges. The overall objective of this study was to use multiple gridded datasets to assess the extent to which the twenty EFRs represent the climate, ecosystem structure, and ecosystem functions of southeastern forests. The EFRs represent the large variability of climate conditions across the region relatively well, but we identified small representation gaps. The representativeness of ecosystem structure by these EFRs can be improved by establishing EFRs in forests with relatively low tree cover, leaf area index, or tree canopy height. The current EFRs also represent the forest ecosystem functions of the region relatively well, although areas with intermediate and low aboveground biomass and water yield are not well represented. The trends in climate, ecosystem structure, and ecosystem functions were generally consistent between the region and the EFRs. Our study indicates that the current EFRs represent the region relatively well, but establishing additional EFRs in specific areas within the region could help more completely assess how southeastern forests respond to climate change, disturbance, and management practices.

Study Implications: This study across the experimental forests and ranges (EFRs) and the southeastern forest region fills the knowledge gap regarding climate, ecosystem structure, and ecosystem functions of EFRs in the context of the broader southeastern forest region. Understanding ecosystem functions and structures across the EFR network can help the Southern Research Station to address new research questions. Our study indicates that the current EFRs represent the climate, ecosystem structure, and ecosystem functions of southeastern forests well. However, establishing additional EFRs in certain regions could help more completely assess how southeastern forests respond to climate change, disturbance, and management practices.
Synthesis estimators for transportability with positivity violations by a continuous covariate
Authors: Zivich Paul N , Edwards Jessie K , Shook-Sa Bonnie E , Lofgren Eric T , Lessler Justin , Cole Stephen R
Published: September 2024

Studies intended to estimate the effect of a treatment, like randomized trials, may not be sampled from the desired target population. To correct for this discrepancy, estimates can be transported to the target population. Methods for transporting between populations are often premised on a positivity assumption, such that all relevant covariate patterns in one population are also present in the other. However, eligibility criteria, particularly in the case of trials, can result in violations of positivity when transporting to external populations. To address nonpositivity, a synthesis of statistical and mathematical models can be considered. This approach integrates multiple data sources (e.g. trials, observational, pharmacokinetic studies) to estimate treatment effects, leveraging mathematical models to handle positivity violations. This approach was previously demonstrated for positivity violations by a single binary covariate. Here, we extend the synthesis approach for positivity violations with a continuous covariate. For estimation, two novel augmented inverse probability weighting estimators are proposed. Both estimators are contrasted with other common approaches for addressing nonpositivity. Empirical performance is compared via Monte Carlo simulation. Finally, the competing approaches are illustrated with an example in the context of two-drug vs. one-drug antiretroviral therapy on CD4 T cell counts among women with HIV.
Bigger, Badder Bugs
Authors: Levinstein Benjamin A , Spencer Jack
Published: September 2024

In this paper we motivate the ‘principles of trust’, chance-credence principles that are strictly stronger than the New Principle yet strictly weaker than the Principal Principle, and argue, by proving some limitative results, that the principles of trust conflict with Humean Supervenience.
Gut Microbial and Metabolic Features Associated With Clostridioides difficile Infection Recurrence in Children
Authors: Li Xiaolu , Xiao Fangfei , Wang Xufei , Ye Lin , Xiao Yongmei , Li Dan , Zhang Ting , Wang Yizhong
Published: September 2024

Background

Recurrent Clostridioides difficile infection (CDI) is a critical clinical issue due to the increase in incidence and difficulty in treatment. We aimed to identify gut microbial and metabolic features associated with disease recurrence in a group of pediatric CDI patients.

Methods

A total of 84 children with primary CDI were prospectively enrolled in the study. Fecal samples collected at the initial diagnosis were subjected to 16S rRNA gene sequencing and targeted metabolomics analysis to profile the bacterial composition and metabolome.

Results

Twenty-six of 84 (31.0%) pediatric CDI patients experienced recurrence. The alpha diversity of the fecal microbiota was significantly lower in the recurrent group than in the nonrecurrent group, and the beta diversity was different from that of the nonrecurrent group. Taxonomic proﬁles revealed that the relative abundances of multiple bacterial taxa significantly differed between the recurrent and nonrecurrent groups. Linear discriminant analysis effect size analysis identified several bacterial genera that discriminated between recurrent and nonrecurrent groups, including Parabacteroides, Coprococcus, Dialister, and Clostridium. Recurrent bacteria presented lower abundances of several short-chain fatty acid (SCFA)–producing bacteria (Faecalibacterium, Butyricicoccus, Clostridium, Roseburia, and Ruminococcus), which were correlated with reduced fecal SCFA levels. In addition, several bile acids, including lithocholic acid (LCA), 12-ketoLCA, trihydroxycholestanoic acid, and deoxycholic acid, were decreased in recurrent patients.

Conclusions

Our study suggests that the differing gut microbiota profiles in pediatric CDI patients may contribute to disease recurrence by modulating SCFA concentrations and bile acid profiles. The gut microbiota and metabolite signatures may be used to predict disease recurrence in children with CDI.
Mantle cell lymphoma in the sphenoid sinus showing acute bilateral blindness
Authors: Kanaya Yosuke , Tsunoda Atsunobu , Saigo Kanako , Tanaka Kumiko , Yoshikawa Akihisa , Kubo Satoko , Matsumoto Fumihiko
Published: September 2024

A 57-year-old man was referred to our department with a mass in the sphenoid sinus. Surgical removal of the tumor was performed. However, a testicular mass was found that showed rapid growth. He had undergone inguinal orchiectomy. Five days after the urological surgery, he noticed visual disturbances and bilateral visual loss. Ophthalmological examination revealed total blindness, and magnetic resonance imaging revealed sphenoid mass growth. The patient underwent emergent removal of the tumor, and a diagnosis of malignant lymphoma was made. A final diagnosis of mantle cell lymphoma (MCL) in the testis and sphenoid sinus was made. After receiving treatment with intravenous corticosteroids and chemotherapy for lymphoma, his left vision completely recovered. Although his right vision was lost, he returned to normal social activities. This is the first report in the literature on MCL developing in the sphenoid sinus presenting with bilateral blindness and ipsilateral recovery.
Sphingolipid homeostasis: How do cells know when enough is enough? Implications for plant pathogen responses
Authors: Cahoon Edgar B , Kim Panya , Xie Tian , González Solis Ariadna , Han Gongshe , Gong Xin , Dunn Teresa M
Published: September 2024

Sphingolipid homeostatic regulation is important for balancing plant life and death. Plant cells finely tune sphingolipid biosynthesis to ensure sufficient levels to support growth through their basal functions as major components of endomembranes and the plasma membrane. Conversely, accumulation of sphingolipid biosynthetic intermediates, long-chain bases (LCBs) and ceramides, is associated with programmed cell death. Limiting these apoptotic intermediates is important for cell viability, while overriding homeostatic regulation permits cells to generate elevated LCBs and ceramides to respond to pathogens to elicit the hypersensitive response in plant immunity. Key to sphingolipid homeostasis is serine palmitoyltransferase (SPT), an endoplasmic reticulum–associated, multi-subunit enzyme catalyzing the first step in the biosynthesis of LCBs, the defining feature of sphingolipids. Across eukaryotes, SPT interaction with its negative regulator Orosomucoid-like (ORM) is critical for sphingolipid biosynthetic homeostasis. The recent cryo-electron microscopy structure of the Arabidopsis SPT complex indicates that ceramides bind ORMs to competitively inhibit SPT activity. This system provides a sensor for intracellular ceramide concentrations for sphingolipid homeostatic regulation. Combining the newly elucidated Arabidopsis SPT structure and mutant characterization, we present a model for the role of the 2 functionally divergent Arabidopsis ceramide synthase classes to produce ceramides that form repressive (trihydroxy LCB-ceramides) or nonrepressive (dihydroxy LCB-ceramides) ORM interactions to influence SPT activity. We describe how sphingolipid biosynthesis is regulated by the interplay of ceramide synthases with ORM-SPT when “enough is enough” and override homeostatic suppression when “enough is not enough” to respond to environmental stimuli such as microbial pathogen attack.
Economic valuation of open research data: A conceptual framework and methodological approach
Authors: Tu Zhifang , Shen Jiashu
Published: September 2024

The economic significance of open research data is widely acknowledged, yet its quantification remains challenging. This paper presents an effective valuation instrument to help stakeholders understand and evaluate the economic benefits of open research data. By conducting a scoping review and prioritizing user engagement, this study introduces a comprehensive conceptual framework for the economic valuation of open research data. The valuation is based on economic value and willingness to pay, employing the Contingent Valuation Method (CVM). It incorporates per-use models (view, download, and request) and periodic subscription models (monthly and yearly). An empirical survey was conducted at the National Basic Science Data Center (NBSDC) in China to verify this framework. Both pricing models, comprising five distinct tactics, was supported by surveyed users. Measuring economic value by views and by year was preferred, while willingness to pay by downloads and by year was considered more reasonable. Overall, the most applicable valuation approach is on a yearly basis. Through this case study at NBSDC, specific pricing tactics were identified, and the total economic value and users’ willingness to pay were assessed. This study is arguably the first to establish a conceptual framework with pricing tactics from a user perspective. This methodological approach for economic valuation of open research data provides evidence and tools for future research, policy formulation, and resource allocation in the context of open science and innovation.
Association Between Juvenile Psychotic Experiences and Problematic Gaming
Authors: Fernandes André , Biokino Renan M , Miguel Andrew C C , Machado Viviane , Koga Gabriela , Fonseca Laís , Pan Pedro M , Roza Thiago Henrique , Salum Giovanni , Passos Ives Cavalcante , Rohde Luis Augusto , Miguel Euripedes Constantino , Ziebold Carolina , Gadelha Ary
Published: September 2024

Background and Hypothesis

Problematic gaming (PG) is an emerging mental health condition associated with significant adverse outcomes. Even though PG has been linked to other psychiatric disorders, its association with psychotic experiences (PEs) remains poorly explored to date. The aim of our study was to examine the association between both conditions in a large Brazilian community sample. We hypothesized that adolescents with PG were more likely to report PE compared with those without the disorder.

Study Design

Our investigation was based on a cross-sectional subsample of a large Brazilian cohort (n = 1616; 13- to 21-year age range). Using the 7-item version of the Game Addiction Scale, participants were classified according to their gaming status: no PG, PG, or gaming addiction (GA). The association between PG, GA, and PE was assessed through linear regression analyses, which were adjusted for the presence of significant covariates, including other psychiatric conditions.

Study Results

9.5% (n = 154) presented PG and 2.7% (n = 43) had GA. 28.0% received any Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV) diagnosis and the mean PE score was 9.39 (SD = 4.35). Participants presenting PG had greater levels of PE, compared with participants with no PG, even controlled by sociodemographic variables and the presence of any DSM-IV diagnosis (b = 0.96, 95% CI = 0.17–1.75, P = .017).

Conclusions

According to our results, PG was significantly associated with PE, even in the presence of other covariates. Although preliminary, these results suggest that PG and PE may have shared neurobiological and/or behavioral pathways.
Elucidation of γ-glutamyl-β-cyanoalanylglycine biosynthesis in mammalian cells by LC-QTOF-MS
Authors: Mochizuki Ryu , Yamagishi Yoshikazu , Ogra Yasumitsu
Published: September 2024

γ-Glutamyl-β-cyanoalanylglycine (gEcnAG) is a glutathione analog in which the cysteine moiety in glutathione is replaced with β-cyanoalanine, a known plant cyanide metabolite. Previously, gEcnAG was detected in the liver of rats and chicks exposed to β-cyanoalanine. We reported the detection of gEcnAG in naïve mammalian cells using liquid chromatography coupled with tandem quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). LC-QTOF-MS analysis enabled high-resolution confirmation (exact mass determination and MS/MS fragmentation) of the gEcnAG structure. The detection of gEcnAG in rat pheochromocytoma (PC12) cells that were not exposed to β-cyanoalanine suggests its endogenous production. Furthermore, the inhibition of myeloperoxidase, an enzyme potentially required for endogenous cyanide generation, decreased gEcnAG levels in PC12 cells. This supports the notion that PC12 cells intrinsically produce cyanide, unlike HepG2 cells, which exhibited lower intracellular gEcnAG levels. Notably, β-cyanoalanine was undetectable in PC12 cells. Moreover, depleting glutathione with buthionine sulfoximine reduced intracellular gEcnAG levels, whereas supplementation with glutathione reduced ethyl ester increased them. These observations suggest that endogenous gEcnAG may be generated from glutathione, potentially through its reaction with endogenous cyanide. Our findings implicate gEcnAG as a possible metabolite of endogenous cyanide.
Phylogenetic evidence of extensive spatial mixing of diverse HIV-1 group M lineages within Cameroon but not between its neighbours
Authors: Godwe Célestin , Goni Oumarou H , San James E , Sonela Nelson , Tchakoute Mérimé , Nanfack Aubin , Koro Francioli K , Butel Christelle , Vidal Nicole , Duerr Ralf , Martin Darren P , de Oliveira Tulio , Peeters Martine , Altfeld Marcus , Ayouba Ahidjo , Ndung’u Thumbi , Tongo Marcel
Published: September 2024

From the perspective of developing relevant interventions for treating HIV and controlling its spread, it is particularly important to comprehensively understand the underlying diversity of the virus, especially in countries where the virus has been present and evolving since the cross-species transmission event that triggered the global pandemic. Here, we generate and phylogenetically analyse sequences derived from the gag-protease (2010 bp; n = 115), partial integrase (345 bp; n = 36), and nef (719 bp; n = 321) genes of HIV-1 group M (HIV-1M) isolates sampled between 2000 and 2022 from two cosmopolitan cities and 40 remote villages of Cameroon. While 52.4% of all sequenced viruses belonged to circulating recombinant form (CRF) 02_AG (CRF02_AG), the remainder were highly diverse, collectively representing seven subtypes and sub-subtypes, eight CRFs, and 36 highly divergent lineages that fall outside the established HIV-1M classification. Additionally, in 77 samples for which at least two genes were typed, 31% of the studied viruses apparently had fragments from viruses belonging to different clades. Furthermore, we found that the distribution of HIV-1M populations is similar between different regions of Cameroon. In contrast, HIV-1M demographics in Cameroon differ significantly from those in its neighbouring countries in the Congo Basin (CB). In phylogenetic trees, viral sequences cluster according to the countries where they were sampled, suggesting that while there are minimal geographical or social barriers to viral dissemination throughout Cameroon, there is strongly impeded dispersal of HIV-1M lineages between Cameroon and other locations of the CB. This suggests that the apparent stability of highly diverse Cameroonian HIV-1M populations may be attributable to the extensive mixing of human populations within the country and the concomitant trans-national movements of major lineages with very similar degrees of fitness; coupled with the relatively infrequent inter-national transmission of these lineages from neighbouring countries in the CB.
The phylogeny and diversification of the western Eurasian Phaedusinae (Gastropoda: Stylommatophora: Clausiliidae)
Authors: Magonyi Nóra M , Fehér Zoltán , Szekeres Miklós , Páll-Gergely Barna
Published: September 2024

The little-known western Eurasian taxa of the Phaedusinae (land snail family Clausiliidae) are Tertiary relicts, which are widely separated geographically from the rest of the subfamily occurring in eastern Eurasia. In order to elucidate the phylogenetic relationships of this group, we carried out molecular analyses with 11 of the 16 genera using nuclear gene sequences. Our results revealed that within the subfamily, Nothoserrulina and Pamphylica, together with Caspiophaedusa, Dobatia, Pravispira, and Serrulina, belong to a clade that is sister to that of all east Eurasian Phaedusinae except Synprosphyma. In contrast, Graecophaedusa, Laeviphaedusa, Pontophaedusella, and Serrulinella, together with Pontophaedusa, belong to paraphyletic lineages diverging between the branching point of these clades and that of the most basal Synprosphyma lineage. Laeviphaedusa and Pontophaedusella represent the earliest western Eurasian lineages, estimated to date back to the Eocene–Oligocene boundary. Comparison of our phylogeny to morphological traits of the genera identified clade-specific character states of the shells and the reproductive organs. Based on fossil records and on palaeogeographical and palaeofloristic data, we also propose a model for the geographical origin and radiation of this subfamily in Eurasia.
Perinatal Naloxone Care Practices: Survey Results From Persons With OUD and Providers
Authors: Nidey Nichole , Kaplan Heather C. , Ford Susan , Prasad Mona , Lannon Carole
Published: September 2024

Objective

Little is known about naloxone care practices for peripartum persons from the patient or provider perspectives. The objective of this study was to survey peripartum persons and providers about naloxone-related practices.

Methods

Individuals who had an OUD diagnosis during a pregnancy and Ohio healthcare professionals who provide care for peripartum patients with OUD and/or infants with prenatal exposure to opioids were eligible for this study. Patient experiences were assessed through a survey codeveloped with members with lived experience of opioid use disorder. Provider perspectives were examined through a survey codeveloped by the Ohio Perinatal Quality Collaborative. Descriptive statistics and logistic regression were used to examine the proportion of participants who received or provided naloxone care practices and the effect on having a naloxone kit during the perinatal period.

Results

Of the 100 peripartum participants with opioid use disorder, 24% reported receiving naloxone from their prenatal care provider and 48% reported ever having a naloxone kit during the perinatal period. Of the 63 maternal care provider participants, 32 (49%) reported discussing or prescribing naloxone to pregnant patients. Of the 62 pediatric provider participants, 10 (16%) reported that they provide naloxone information to parenting individuals of their patients.

Conclusion

Study results demonstrate critical gaps in naloxone care practices for peripartum persons, emphasizing the need for targeted interventions at the patient, clinician, practice, and system levels.
Cannabinoid Hyperemesis Syndrome Presumed Secondary to CBD Use: A Case Report
Authors: Lefebvre Emilie , Simons Luc , Duval Mélanie , Laforgue Edouard-Jules , Victorri-Vigneau Caroline
Published: September 2024

Introduction

Cannabinoid hyperemesis syndrome is characterized by recurrent episodes of severe nausea and vomiting, often associated with prolonged and excessive cannabis use. With the recent legalization and rising consumption of cannabidiol (CBD) in Europe and the United States, concerns have emerged about its potential role in triggering similar symptoms.

Case Report

A 32-year-old male with a history of cannabis, tobacco and alcohol use disorder experienced multiple cyclic vomiting episodes after switching from cannabis to CBD. Initially, the patient presented with abdominal pain and vomiting after ceasing cannabis use, with symptoms alleviated by hot showers. Three months later, similar symptoms reappeared despite abstinence from cannabis but regular CBD consumption. Over the next 6 months, recurrent episodes of abdominal pain and vomiting persisted with daily CBD use but no cannabis consumption. Clinical data, laboratory results, and treatment responses were analyzed to investigate the connection between CBD consumption and symptom onset.

Discussion

The pathophysiology of cannabis-induced cyclic vomiting is poorly understood. Hypotheses include tetrahydrocannabinol accumulation in adipose tissue, pyrolytic conversion of CBD into tetrahydrocannabinol, and CBD’s intrinsic effects, particularly its interaction with transient receptor potential vanilloid 1 receptors. Our analysis suggests that high doses of CBD may activate transient receptor potential vanilloid 1 receptors, inducing proemetic effects.

Conclusions

Although the connection between CBD and cyclic vomiting remains uncertain, it warrants further investigation. The increasing use of CBD, perceived as a safe dietary supplement, underscores the need to understand its potential health impacts better.
Effects of High Temperature and High Humidity on the Degree of Ocular Damage Caused by 60 GHz Millimeter Wave Exposure
Authors: Kojima Masami , Tasaki Takafumi , Kamijo Toshio , Hada Aki , Suzuki Yukihisa , Ikehata Masateru , Sasaki Hiroshi
Published: September 2024

Millimeter waves (MMW) are pervasive in society; however, studies on the biological effects of MMW exposure are usually performed in laboratory settings not reflecting global environmental diversity. We investigated the effects of a 6-min exposure to 60 GHz MMW (wavelength, 5.0 mm) at incident power densities of 200 and 300 mW cm−2 in eyes (exposed right eyes vs. unexposed left eyes) under various ambient temperature/relative humidity environments (24 °C/50%, 45 °C/20%, and 45 °C/80%) using an in vivo rabbit model. Correlations were examined with adverse ocular events, including corneal epithelial damage (assessed using fluorescein staining), corneal opacity (evaluated by slit-lamp microscopy), and corneal thickness (measured via optical coherence tomography). Our findings indicate that higher temperatures and humidity tend to exacerbate MMW-induced ocular damage, albeit not significantly in the present study. Further research with a larger sample size is warranted. Incident power density emerged as a factor that was directly linked to the ocular damage threshold. High ambient temperature and humidity tended to exacerbate ocular damage from MMW exposure, although the effect was secondary. Ocular damage in a high-temperature (45 °C), high-humidity (80%) environment was increased to the same extent as that by incident power density increased by approximately 100 mW cm−2 in an ocular damage model in a standard environment (24 °C, 50%). In a high-humidity environment, the internal ocular tissue temperature increased at a high ambient temperature of 45 °C, suggesting that the eyeball may respond differently compared to other tissues.
Time to cycle regularity and health risks
Authors: Naveed Amna , Whooten Rachel
Published: September 2024

Purpose of review

Adolescents often have irregular menstrual cycles after menarche until the reproductive axis fully matures. This review explores how menstrual regularity is established and the health risks associated with prolonged time to cycle regularity in adolescents.

Recent findings

Cross-sectional studies show an association between prolonged time to cycle regularity in adolescents and increased risks for ongoing menstrual dysfunction, cardiometabolic disorders, cancers, and overall mortality. Importantly, some of these cardiometabolic associations are independent of PCOS status.

The menstrual cycle can be used as a vital sign for assessing overall health. While further longitudinal studies are needed to establish causal relationships, these findings highlight a crucial window for early intervention in adolescents with prolonged time to cycle regularity to mitigate future risks.
‘Misdiagnosis of diffuse large B-cell lymphoma in the maxillary sinus as a dental abscess’
Authors: Jabr Obai , Haddad Sentia , Almohareb Mumdouh , Al-Rashdan Omar , Basha Zein , Haddad Sultaneh , Jaber Fouad , Jasim Radhi Ali Walaa M. , Alnour Amirah
Published: September 2024

Introduction:

Diffuse large B-cell Lymphoma (DLBCL) is a heterogeneous group of malignant tumors with diverse prognoses, histological appearances, and clinical symptoms. While it commonly affects lymphoid tissues, its occurrence in the nasal cavity and paranasal sinuses is rare and poses significant diagnostic challenges.

Case Presentation:

The authors present a case of a 53-year-old man with a history of testicular non-Hodgkin lymphoma treated 5 months prior. He presented with symptoms initially misdiagnosed as a dental abscess, which was later identified as DLBCL in the maxillary sinus.

Discussion:

The etiology of primary lymphoma in the oral region remains unclear. Due to its rarity, specific prognostic factors, and optimal treatment strategies are not well-established. This case highlights the diagnostic challenges and underscores the importance of considering lymphoma in the differential diagnosis of nonresponsive dental abscesses.

Conclusion:

This case underscores the importance of thorough investigation in patients with nonresponsive dental abscesses. Early identification and appropriate management of DLBCL in uncommon sites require heightened clinical awareness and interdisciplinary collaboration. The optimal treatment approach remains debated and warrants further research.