bluuebunny/crossref_metadata_2025 huggingface dataset:
The Conformation of the N-Terminal Tails of Deinococcus grandis Dps Is Modulated by the Ionic Strength
Authors: Guerra João P. L. , Blanchet Clement E. , Vieira Bruno J. C. , Almeida Ana V. , Waerenborgh João C. , Jones Nykola C. , Hoffmann Søren V. , Tavares Pedro , Pereira Alice S.
Published: April 2022

DNA-binding proteins from starved cells (Dps) are homododecameric nanocages, with N- and C-terminal tail extensions of variable length and amino acid composition. They accumulate iron in the form of a ferrihydrite mineral core and are capable of binding to and compacting DNA, forming low- and high-order condensates. This dual activity is designed to protect DNA from oxidative stress, resulting from Fenton chemistry or radiation exposure. In most Dps proteins, the DNA-binding properties stem from the N-terminal tail extensions. We explored the structural characteristics of a Dps from Deinococcus grandis that exhibits an atypically long N-terminal tail composed of 52 residues and probed the impact of the ionic strength on protein conformation using size exclusion chromatography, dynamic light scattering, synchrotron radiation circular dichroism and small-angle X-ray scattering. A novel high-spin ferrous iron-binding site was identified in the N-terminal tails, using Mössbauer spectroscopy. Our data reveals that the N-terminal tails are structurally dynamic and alter between compact and extended conformations, depending on the ionic strength of the buffer. This prompts the search for other physiologically relevant modulators of tail conformation and hints that the DNA-binding properties of Dps proteins may be affected by external factors.
The Link between Activities of Hepatic 11beta-Hydroxysteroid Dehydrogenase-1 and Monoamine Oxidase-A in the Brain Following Repeated Predator Stress: Focus on Heightened Anxiety
Authors: Tseilikman Vadim , Lapshin Maxim , Klebanov Igor , Chrousos George , Vasilieva Maria , Pashkov Anton , Fedotova Julia , Tseilikman David , Shatilov Vladislav , Manukhina Eugenia , Tseilikman Olga , Sarapultsev Alexey , Downey H. Fred
Published: April 2022

We investigated the presence of a molecular pathway from hepatic 11-βHSD-1 to brain MAO-A in the dynamics of plasma corticosterone involvement in anxiety development. During 14 days following repeated exposure of rats to predator scent stress for 10 days, the following variables were measured: hepatic 11-βHSD-1 and brain MAO-A activities, brain norepinephrine, plasma corticosterone concentrations, and anxiety, as reflected by performance on an elevated plus maze. Anxiety briefly decreased and then increased after stress exposure. This behavioral response correlated inversely with plasma corticosterone and with brain MAO-A activity. A mathematical model described the dynamics of the biochemical variables and predicted the factor(s) responsible for the development and dynamics of anxiety. In the model, hepatic 11-βHSD-1 was considered a key factor in defining the dynamics of plasma corticosterone. In turn, plasma corticosterone and oxidation of brain ketodienes and conjugated trienes determined the dynamics of brain MAO-A activity, and MAO-A activity determined the dynamics of brain norepinephrine. Finally, plasma corticosterone was modeled as the determinant of anxiety. Solution of the model equations demonstrated that plasma corticosterone is mainly determined by the activity of hepatic 11-βHSD-1 and, most importantly, that corticosterone plays a critical role in the dynamics of anxiety following repeated stress.
Having a Same Type IIS Enzyme’s Restriction Site on Guide RNA Sequence Does Not Affect Golden Gate (GG) Cloning and Subsequent CRISPR/Cas Mutagenesis
Authors: Moniruzzaman M. , Zhong Yun , Huang Zhifeng , Zhong Guangyan
Published: April 2022

Golden gate/modular cloning facilitates faster and more efficient cloning by utilizing the unique features of the type IIS restriction enzymes. However, it is known that targeted insertion of DNA fragment(s) must not include internal type IIS restriction recognition sites. In the case of cloning CRISPR constructs by using golden gate (GG) cloning, this narrows down the scope of guide RNA (gRNA) picks because the selection of a good gRNA for successful genome editing requires some obligation of fulfillment, and it is unwanted if a good gRNA candidate cannot be picked only because it has an internal type IIS restriction recognition site. In this article, we have shown that the presence of a type IIS restriction recognition site in a gRNA does not affect cloning and subsequent genome editing. After each step of GG reactions, correct insertions of gRNAs were verified by colony color and restriction digestion and were further confirmed by sequencing. Finally, the final vector containing a Cas12a nuclease and four gRNAs was used for Agrobacterium-mediated citrus cell transformation. Sequencing of PCR amplicons flanking gRNA-2 showed a substitution (C to T) mutation in transgenic plants. The knowledge derived from this study could widen the scope of GG cloning, particularly of gRNAs selection for GG-mediated cloning into CRISPR vectors.
An Overview of Cotton Gland Development and Its Transcriptional Regulation
Authors: Jan Masood , Liu Zhixin , Guo Chenxi , Zhou Yaping , Sun Xuwu
Published: April 2022

Cotton refers to species in the genus Gossypium that bear spinnable seed coat fibers. A total of 50 species in the genus Gossypium have been described to date. Of these, only four species, viz. Gossypium, hirsutum, G. barbadense, G. arboretum, and G. herbaceum are cultivated; the rest are wild. The black dot-like structures on the surfaces of cotton organs or tissues, such as the leaves, stem, calyx, bracts, and boll surface, are called gossypol glands or pigment glands, which store terpenoid aldehydes, including gossypol. The cotton (Gossypium hirsutum) pigment gland is a distinctive structure that stores gossypol and its derivatives. It provides an ideal system for studying cell differentiation and organogenesis. However, only a few genes involved in the process of gland formation have been identified to date, and the molecular mechanisms underlying gland initiation remain unclear. The terpenoid aldehydes in the lysigenous glands of Gossypium species are important secondary phytoalexins (with gossypol being the most important) and one of the main defenses of plants against pests and diseases. Here, we review recent research on the development of gossypol glands in Gossypium species, the regulation of the terpenoid aldehyde biosynthesis pathway, discoveries from genetic engineering studies, and future research directions.
The Associations of Mental Health Disturbances, Self-Esteem, and Partner Violence Victimization with Condom Use in Spanish Adolescents
Authors: Sánchez-SanSegundo Miriam , Alarcó-Rosales Raquel , Zaragoza-Martí Ana , Quesada-Rico José A. , Gabaldón-Bravo Eva , Hurtado-Sánchez José A.
Published: April 2022

Risky sexual behaviors, such as not using a condom for preventing sexually transmittable diseases and unwanted pregnancies, are associated with emotional and psychological problems in adolescence, including higher levels of depression, anxiety, stress, and low self-esteem. Adolescents with a history of violence victimization are also at increased risk of engaging in sexual risk behaviors. In this study, we examined the associations between mental health disturbances and partner violence victimization and the non-use of condoms among young people in Spain. We also examined self-esteem as a mediator of mental health problems and unprotected sexual relationships. A cross-sectional study was conducted on a sample of 831 adolescents randomly selected from 25 high schools in Spain. From the initial sample, 285 students (144 men and 141 women) from high schools in Alicante (Spain) who reported having experienced sexual activity with at least one partner were approached. The results showed that one in three adolescents between 14 and 19 years old did not use condoms during sexual intercourse. The factors associated with the non-use of condoms in the estimated models of Poisson robust variance were having a history of physical, psychological, or sexual violence; having been expelled from school because of behavioral problems; and having higher levels of depression, anxiety, and stress and lower levels of self-esteem. Self-esteem was independently associated with the non-use of condoms. Having lower levels of self-esteem increased the risk of not using a condom during participants’ last sexual intercourse. These findings suggest the importance of developing public health strategies for mental health promotion to increase condom use among adolescents.
Effects of Music Volume Preference on Endurance Exercise Performance
Authors: Nixon Kylie M. , Parker Mckenzie G. , Elwell Carson C. , Pemberton Anna L. , Rogers Rebecca R. , Ballmann Christopher G.
Published: April 2022

The purpose of this study was to examine the effects of preferred versus non-preferred music volume on relative power output, trial time to completion (TTC), heart rate (HR), rate of perceived exertion (RPE), and motivation during endurance rowing exercise. Physically active females (age 18–25) volunteered to participate. In a crossover counterbalanced design, participants completed two trials: non-preferred (NPV) and preferred (PV) music volume. Participants began with a rowing warm-up at 50% of HRmax for 5 min. Following this, participants completed a 2000 m rowing time trial as quickly as possible. Relative power output, HR, and RPE were documented each minute during the exercise bout. TTC and motivation levels were documented at the cessation of exercise. Results showed that there were no significant differences between NPV and PV for relative power output (p = 0.287; d = 0.17), TTC (p = 0.816; d = 0.01), and HR (p = 0.956; d = 0.08). However, RPE was significantly lower (p = 0.002; d = 0.86) and motivation was significantly higher (p < 0.001; d = 2.14) during the PV condition versus NPV. Findings suggest that while PV does not impart performance-enhancing effects during endurance exercise compared to NPV, it may improve psychological responses related to intensity and effort which could have important implications for enduring intense exercise and training.
Understanding the Driving Factors for Urban Human Settlement Vitality at Street Level: A Case Study of Dalian, China
Authors: Liu He , Li Xueming
Published: April 2022

Vitality can effectively test the quality of regional space, put forward the concept of urban human settlement vitality, and explore the development status of urban human settlement vitality space, which is of great significance in promoting the high-quality development of urban human settlements. By constructing an evaluation index system of urban human settlement vitality and comprehensively using projection pursuit models, spatial correlation analysis, and spatial measurement models, the spatial pattern and influencing factors of the vitality of urban human settlements in the four districts of Dalian were studied. The results are as follows: (1) The spatial differentiation characteristics of the vitality of urban human settlements in Dalian are remarkable. Overall, it gradually decreased from the city center to the administrative boundary. (2) The spatial dependence of the vitality of urban human settlements among regions is relatively strong, with a more obvious “Matthew effect”. Among them, urban human settlement vitality hot spots were mainly distributed in the southeast of Dalian, showing a concentrated distribution trend, while the cold spots were distributed in the northern fringe area of Dalian, with spatial homogeneity characteristics. (3) Topography, ecological environment, social economy, commercial development, spatial structure, spatial form, regional scale, etc. have different impacts on the vitality of urban human settlements.
Multi-Scenario Simulation Analysis of Land Use and Carbon Storage Changes in Changchun City Based on FLUS and InVEST Model
Authors: Li Yingxue , Liu Zhaoshun , Li Shujie , Li Xiang
Published: April 2022

Land use change is an important reason for changes in carbon storage in terrestrial ecosystems. Therefore, analyzing the impact of land use change on carbon storage is important for exploring the sustainable development of cities and improving the value of ecosystem services. Taking Changchun City in the northeast of China as the research area, this paper simulates land use patterns under three scenarios up to 2030 using the FLUS model and assesses carbon storage from 2010 to 2030 using the InVEST model. It estimates the impact of land use change on carbon storage under several scenarios in Changchun. The results show that cultivated land plays an important role in carbon storage in Changchun. The transfer of cultivated land to construction land has been the main land use type conversion over the past decade, which has led to most of the carbon storage loss. In the natural growth scenario, the carbon storage would decline further. In the cultivated land protection scenario, meanwhile, this situation would be greatly improved. In the ecological protection scenario, the carbon storage would be increased due to the protection of ecological land. In the future, we should protect existing resources while simultaneously comprehensively improving the economic, social, and ecological benefits of the land.
Beliefs, Commitments, and Ad Baculum Arguments
Authors: Casey John
Published: April 2022

Typically, an ad baculum argument is one where an arguer threatens a respondent in order to induce them to adopt a standpoint. It is a fallacy, a common account goes, because the power to impose a standpoint is irrelevant to its truth or acceptability. However, fallacies, if they are to be anything, ought at a minimum to be persuasive, and it is hard to see how an ad baculum might persuade. Employing an ad baculum just underscores how terrible someone’s reasons are. Despite this, cases of fallacious ad baculum arguments seem to exist, and this is a fact that requires some explanation. This paper offers an account where the real target of an ad baculum is an audience downstream from the initial ad baculum exchange. This means that the ad baculum consists of misrepresenting the quality of evidence by means of the forced adoption of a particular standpoint.
Effect of Multidirectional Isothermal Forging on Microstructure and Mechanical Properties in Ti-6Al-4V Alloy
Authors: Xu Zhichao , Yang Wenju , Fan Jianfeng , Wu Tao , Gao Zeng
Published: April 2022

In the present work, the microstructure and mechanical properties of Ti-6Al-4V alloy during multidirectional isothermal forging (MDIF) were systematically investigated. The evolution of the microstructure and texture of Ti-6Al-4V alloy during MDIF was studied using TEM and electron backscattered diffraction (EBSD). The experiment results showed that the grain size decreased with the increase in cumulative strain, especially in the easy deformation zone. After four deformation cycles, a homogeneous equiaxed grained microstructure with an average grain size of 0.14 μm was achieved. The texture changes of the alloy were studied in detail. After one cycle of MDIF, the texture was mainly composed of (0002) [01 10], and the Euler angles were (8°, 30°, 30°). The density of texture decreased with the increase in loading cycle, but the dispersion of texture increased. After four cycles of MDIF, the non-basal texture (1010) <1102> texture was observed, and the Euler angles were (82°, 33°, 0°). The highest achieved mechanical properties for Ti-6Al-4V alloy in the MDIF condition were a yield strength 900 MPa, ultimate tensile strength of 921 MPa, and an elongation of 12.1% at room temperature. The increase in MDIF cycles improved the hardness of the alloy. The significant improvement in mechanical properties was attributed to the ultrafine-grained microstructure.
Influence of Woven-Fabric Type on the Efficiency of Fabric-Reinforced Polymer Composites
Authors: Axinte Andrei , Ungureanu Dragoș , Țăranu Nicolae , Bejan Liliana , Isopescu Dorina Nicolina , Lupășteanu Radu , Hudișteanu Iuliana , Roșca Victoria Elena
Published: April 2022

The greatest advantage of fiber-reinforced composite materials is the freedom to tailor their strength and stiffness properties, while the most significant disadvantage consists in their high costs. Therefore, the design process and especially the optimization phase becomes an important step. The geometry of the fabric of each lamina as well as their stacking sequence need to be carefully defined, starting from some basic geometric variables. The input parameters are the widths and the heights of the tows, the laminate-stacking sequence and the gaps between two successive tows or the height of the neat matrix. This paper is a follow-up to a previous work on using and improving an in-house software called SOMGA (Satin Optimization with a Modified Genetic Algorithm), aimed to optimize the geometrical parameters of satin-reinforced multi-layer composites. The final goal is to find out the way in which various types of woven fabrics can affect the best possible solution to the problem of designing a composite material, able to withstand a given set of in-plane loads. The efficiency of the composite structure is evaluated by its ultimate strains using a fitness function that analyses and compares the mechanical behavior of different fabric-reinforced composites. Therefore, the ultimate strains corresponding to each configuration are considered intermediate data, being analyzed comparatively until obtaining the optimal values. When the software is running, for each analysis step, a set of intermediate values is provided. However, the users do not have to store these values, because the final result of the optimization directly provides the composite configuration with maximum efficiency, whose structural response meets the initially imposed loading conditions. To illustrate how the SOMGA software works, six different satin-woven-fabric-reinforced composites, starting from plain weave (satin 2/1/1), then satin 3/1/1, satin 4/1/1, satin 5/1/1, satin 5/2/1 and finally satin 5/3/1, were evaluated in the SOMGA interface. The results were rated against each other in terms of the composite efficiency and the case characterized by minimal reinforcement undulation (thinnest laminate) were highlighted.
Evaluation of the Radiation Shielding Properties of a Tellurite Glass System Modified with Sodium Oxide
Authors: Hussein Khalid I. , Alqahtani Mohammed S. , Meshawi Arwa A. , Alzahrani Khloud J. , Zahran Heba Y. , Alshehri Ali M. , Yahia Ibrahim S. , Reben Manuela , Yousef El Sayed
Published: April 2022

In this study, the X-ray and gamma attenuation characteristics and optical properties of a synthesized tellurite–phosphate–sodium oxide glass system with a composition of (85 − x)TeO2–10P2O5–xNa2O mol% (where x = 15, 20, and 25) were evaluated. The glass systems we re fabricated by our research group using quenching melt fabrication. The shielding parameters of as-synthesized systems, such as the mass attenuation coefficient (MAC), linear attenuation coefficient (LAC), effective atomic number (Zeff), half-value layer (HVL), tenth value layer (TVL), mean free path (MFP), and effective electron density (Neff) in a wide energy range between 15 keV and 15 MeV, were estimated using well-known PHY-X/PSD software and recently developed MIKE software. Herein, the optical parameters of prepared glasses, such as molar volume (VM), oxygen molar volume (VO), oxygen packing density (OPD), molar polarizability (αm), molar refractivity (Rm), reflection loss (RL), and metallization (M), were estimated using MIKE software. Furthermore, the shielding performance of the prepared glasses was compared with that of commonly used standard glass shielding materials. The results show that the incorporation of sodium oxide into the matrix TeO2/P2O5 with an optimum concentration can yield a glass system with good shielding performance as well as good optical and physical properties, especially at low photon energy.
Mechanism and Internal Stability of Supportive Stone Constructions
Authors: Voit Klaus , Hron Johannes , Frei Gerhard , Adamcova Renata , Zeman Oliver
Published: April 2022

Natural stone constructions for the protection of slopes, banks and riverbeds are widely used in infrastructure engineering. These structures are made of stacked natural stones, which can be placed loosely on top of each other. Additionally, their bond behavior can be improved by using concrete mortar to fill the joints between the stones. Although such structures are now widely used, there is still a need for research concerning their inner stability and the structural design of such protective stone structures. In this study, experimentally, investigations were made to determine the force transmission and the interaction between rock and concrete mortar by deriving characteristic values of the adhesion strength and friction angle at different scales. A method for the determination of shear parameters from direct shear testing is used, considering the interaction between vertical and horizontal forces in the joint. In the course of these investigations, the roughness of the rock surface was recorded using conventional visual methods using the joint roughness coefficient (JRC) as well as via laser imaging. By applying laser scanning, a theoretical roughness factor could be derived. Furthermore, the properties of the rocks of the concrete mortar (fresh and hardened concrete mortar properties as well as a durability characteristic) were investigated in detail. It could be shown that different types of concrete mortar result in different bond strengths—expressed as tensile and shear strengths—when applied to a stone surface. The roughness of the stone surface has a positive influence on the tensile and shear strength between the stone and the mortar. Based on the test results, a failure description based on the Mohr–Coulomb fracture criterion could be determined, which can be used to calculate characteristic parameters for the design of stone support bodies. It was also shown that the stone’s compressive strength is being exceeded through load due to very punctual contact areas. Moreover, concrete mortar differs significantly from conventional concrete in terms of its mechanical properties due to the on-site installation conditions, which allow no dynamic compaction.
Majorization–Minimization Total Variation Solution Methods for Electrical Impedance Tomography
Authors: Alruwaili Eman , Li Jing
Published: April 2022

Inverse problems arise in many areas of science and engineering, such as geophysics, biology, and medical imaging. One of the main imaging modalities that have seen a huge increase in recent years is the noninvasive, nonionizing, and radiation-free imaging technique of electrical impedance tomography (EIT). Other advantages of such a technique are the low cost and ubiquitousness. An imaging technique is used to recover the internal conductivity of a body using measurements from electrodes from the body’s surface. The standard procedure is to obtain measurements by placing electrodes in the body and measuring conductivity inside the object. A current with low frequency is applied on the electrodes below a threshold, rendering the technique harmless for the body, especially when applied to living organisms. As with many inverse problems, EIT suffers from ill-posedness, i.e., the reconstruction of internal conductivity is a severely ill-posed inverse problem and typically yields a poor-quality solution. Moreover, the desired solution has step changes in the electrical properties that are typically challenging to be reconstructed by traditional smoothing regularization methods. To counter this difficulty, one solves a regularized problem that is better conditioned than the original problem by imposing constraints on the regularization term. The main contribution of this work is to develop a general ℓp regularized method with total variation to solve the nonlinear EIT problem through a iteratively reweighted majorization–minimization strategy combined with the Gauss–Newton approach. The main idea is to majorize the linearized EIT problem at each iteration and minimize through a quadratic tangent majorant. Simulated numerical examples from complete electrode model illustrate the effectiveness of our approach.
A Natural Approximation to the Complete Elliptic Integral of the First Kind
Authors: Zhu Ling
Published: April 2022

Let K(r) be the complete elliptic integral of the first kind. Then, the inequality 2K(r)/π>tanh−1(r)/sin−1r holds for all r∈(0,1). This conclusion does not match those in the existing literature.
Genome Mining of α-Pyrone Natural Products from Ascidian-Derived Fungus Amphichordafelina SYSU-MS7908
Authors: Yuan Siwen , Chen Litong , Wu Qilin , Jiang Minghua , Guo Heng , Hu Zhibo , Chen Senhua , Liu Lan , Gao Zhizeng
Published: April 2022

Culturing ascidian-derived fungus Amphichorda felina SYSU-MS7908 under standard laboratory conditions mainly yielded meroterpenoid, and nonribosomal peptide-type natural products. We sequenced the genome of Amphichorda felina SYSU-MS7908 and found 56 biosynthetic gene clusters (BGCs) after bioinformatics analysis, suggesting that the majority of those BGCSs are silent. Here we report our genome mining effort on one cryptic BGC by heterologous expression in Aspergillus oryzae NSAR1, and the identification of two new α-pyrone derivatives, amphichopyrone A (1) and B (2), along with a known compound, udagawanone A (3). Anti-inflammatory activities were performed, and amphichopyrone A (1) and B (2) displayed potent anti-inflammatory activity by inhibiting nitric oxide (NO) production in RAW264.7 cells with IC50 values 18.09 ± 4.83 and 7.18 ± 0.93 μM, respectively.
Predictive Factors of Functioning Adrenal Incidentaloma: A 15-Year Retrospective Study
Authors: Muangnoo Natwara , Manosroi Worapaka , Leelathanapipat Napitch , Meejun Tanaporn , Chowchaiyaporn Pattanan , Teetipsatit Pasinee
Published: April 2022

Background and Objectives: Adrenal incidentaloma is an adrenal mass that is found incidentally in abdominal imaging studies. It is important to categorize whether the mass is a functioning or non-functioning incidentaloma to determine the appropriate management and follow-up. Our study aimed to identify predictive factors for functioning adrenal incidentaloma that could assist in early detection and in determining which patients may require hormonal investigations. Materials and methods: This 15-year retrospective study was performed in a tertiary care medical center. A total of 244 patients with adrenal incidentaloma were identified, of whom 88 had functioning adrenal incidentaloma. The patients’ clinical biochemical factors and radiographic parameters were reviewed. Multivariable analysis using logistic regression with backward stepwise selection analysis was performed. Results are presented as odds ratios (ORs) with 95% confidence interval (CI). Statistical significance was set at p < 0.05. Results: A significant clinical predictor for functioning adrenal incidentaloma is a history of hypertension (OR 2.72, 95% CI(1.53, 4.82)), while significant radiological predictors include mass size >4 cm (OR 2.20, 95% CI(1.20, 4.02)) and Hounsfield units (HU) < 10 (OR 2.47, 95% CI(1.23, 4.94)). Conclusions: These easy-to-obtain clinical and radiological predictors can be used to help identify functioning adrenal incidentaloma. In clinical practice, taking these factors into consideration could potentially reduce the number of investigations required to diagnose functioning adrenal incidentaloma.
Current and Future Role of Neoadjuvant Chemoimmunotherapy for Early Triple-Negative Breast Cancer: Which Way to Go Forward
Authors: Rizzo Alessandro , Cusmai Antonio , Gadaleta-Caldarola Gennaro , Palmiotti Gennaro
Published: April 2022

Immunotherapy has revolutionized previous triple-negative breast cancer (TNBC) treatment algorithms, prompting researchers and clinicians to consider the expansion of the role of immunotherapy in other settings, including the earlier stage of the disease (e.g., as neoadjuvant and adjuvant therapy). The role of chemoimmunotherapy have been assessed in some recently presented and published clinical trials, including the KEYNOTE-522, the IMpassion031, and the GeparNUEVO. In the current Editorial, we will provide a critical snapshot of these studies, exploring strengths and limitations of neoadjuvant chemotherapy in early TNBC.
Treatment of Class III Malocclusion and Anterior Crossbite with Aligners: A Case Report
Authors: Inchingolo Alessio , Patano Assunta , Coloccia Giovanni , Ceci Sabino , Inchingolo Angelo , Marinelli Grazia , Malcangi Giuseppina , Di Pede Chiara , Garibaldi Mariagrazia , Ciocia Anna , Mancini Antonio , Palmieri Giulia , Rapone Biagio , Piras Fabio , Cardarelli Filippo , Nucci Ludovica , Bordea Ioana , Scarano Antonio , Lorusso Felice , Giovanniello Delia , Costa Stefania , Tartaglia Gianluca , Di Venere Daniela , Dipalma Gianna , Inchingolo Francesco
Published: April 2022

The article describes the orthodontically treated case of a 25-year-old patient with skeletal and dental class III malocclusion, anterior crossbite, which caused functional and aesthetic problems, occlusal trauma, and incisor wear. Treatment with transparent aligners was proposed to meet the patient’s needs, using the sequential distalization protocol. While sequential distalization is well documented for class II malocclusion treatment in maxillary arch teeth, further investigations are necessary for class III malocclusions. In fact, lower teeth movements are more complex due to mandibular bone density and the presence of the third molars, which are often extracted to perform distalization. In addition, the use of intermaxillary elastics helps control the proclination of the anterior teeth as a reaction to distalizing forces. At the end of the treatment, the patient reached molar and canine class I and positive overjet and overbite. The inclination of lower incisors and the interincisal angle have improved, resulting in aesthetic and functional enhancement.
Intrapericardial Thymoma Presented as Pericardial Tamponade with Post-Operative Myasthenia Gravis
Authors: Tsai Yueh-Hsun , Ko Kai-Hsiung , Yen Hao , Huang Tsai-Wang
Published: April 2022

Background: Thymoma is an epithelial mass arising from the thymus. Most thymomas are located in the anterior mediastinum. Ectopic intrapericardial thymoma is very unusual; to date, only eight cases of pericardial thymoma have been reported. Among thymoma patients, 20% to 25% are associated with myasthenia gravis. However, postoperative myasthenia gravis occurs in less than 1% of cases. Here, we share a rare case of ectopic intrapericardial thymoma that developed postoperative myasthenia gravis six months after surgery. Case presentation: A 66-year-old woman visited the outpatient department due to productive cough and chest pain. Chest radiography showed increased soft tissue opacity over the mediastinum. A soft tissue mass in the pericardium and a ground glass nodule in right upper lung were noted using chest computed tomography. The diagnosis of thymoma, type B2, pT3N0M0, and stage IIIA and synchronous adenocarcinoma in situ of the right upper lung was confirmed after surgical removal. Six months later, the patient developed postoperative myasthenia gravis. Conclusions: Thymoma is rarely considered a differential diagnosis in pericardial tumors. Surgical removal with adjuvant radiation therapy should be performed considering the malignancy potential of thymomas and cardiac complications. In patients without myasthenia gravis, a small chance of postoperative myasthenia gravis remains. Patients should be carefully monitored for myasthenia gravis after surgery.
Studying the Effect of Shortening Carbon Nanotubes via Ball Milling on Cellulose Acetate Nanocomposite Membranes for Desalination Applications
Authors: Elbadawi Nouran A. , Ramadan Adham R. , Esawi Amal M. K.
Published: April 2022

Studying the effect of different sizes of multi-walled carbon nanotubes (CNTs) on mixed matrix membranes in nanofiltration applications has not been widely reported in the literature. In this study, two different lengths of functionalized CNTs were used to investigate such effect. First, CNTs were shortened by using high-energy ball milling at 400 RPM, with a ball-to-powder weight ratio (BPR) of 120:1. Characterization of the structure of the CNTs was carried out using TEM, XRD, SEM, BET, and Raman Spectroscopy. Second, 0.001 wt % of unmilled and milled CNTs were incorporated into cellulose acetate nanocomposite membranes, Eli-0 (unmilled), and Eli-400 (milled at 400 RPM) to study their effects on the membranes’ morphology, porosity, hydrophilicity, and performance analysis in terms of permeation and salt retention rates of 5000 ppm Na2SO4. Results showed that shortening CNTs enhanced the membranes’ hydrophilicity and affected macrovoid and micropore formation. Furthermore, shortening CNTs resulted in opening their caps and improved the permeation rates with a slight adverse effect on salt retention.
Microstructure and Superplastic Behavior of Ni-Modified Ti-Al-Mo-V Alloys
Authors: Kotov Anton D. , Postnikova Maria N. , Mosleh Ahmed O. , Cheverikin Vladimir V. , Mikhaylovskaya Anastasia V.
Published: April 2022

The paper studies the influence of 0.5–1.8 wt.% Ni alloying on the superplasticity, microstructural evolution, and dynamic grain growth effect in a temperature range of 625–775 °C and room temperature mechanical properties of two-phase Ti-Al-Mo-V alloys. Due to a decrease in β transus and an enhancement in the alloy diffusivity, an increase in Ni content significantly improved superplasticity. The Ni-modified alloys exhibited 1.5–3-fold lower flow stress, a 2.5–3-fold greater elongation to failure, and 1.4–1.7-fold higher strain rate sensitivity m coefficient compared to the Ni-free alloy. An intermetallic Ti2Ni compound precipitated in the 1.8 wt.% Ni-modified alloy during low-temperature deformation at 700 °C and decreased superplastic properties. The Ti-4Al-3Mo-1V-0.1B alloy with 0.9 wt.% Ni exhibited a good combination of the superplastic behavior and room-temperature mechanical properties: an elongation to failure of 500–900% at a low-temperature range of 625–775 °C and constant strain rate of 1 × 10−3 s−1 and a yield strength of 885 MPa and ultimate tensile strength of 1020 MPa after pre-straining for 100% in a superplastic regime and strengthening heat treatment.
Metabolites Alterations and Liver Injury in Hepatic Encephalopathy Models Evaluated by Use of 7T-MRI
Authors: Saito Shigeyoshi , Arihara Narumi , Sawaya Reika , Morimoto-Ishikawa Daisuke , Ueda Junpei
Published: April 2022

This study is to observe a thioacetamide (TAA) administered Hepatic encephalopathy (HE) model rats at three and ten days after TAA administration using liver MRI and brain MR Spectroscopy (MRS) by use of 7T-MRI. Forty-two Wistar rats (control group, n = 14) were intraperitoneally administered at 300 mg/kg (low-dose group, n = 14) or 400 mg/kg (high-dose group, n = 14) doses of TAA for induced of HE. At three days after TAA administration, glutamine (Gln) measured by MRS in high-dose and low-dose TAA groups showed significant increases in comparison to those of the control group (p < 0.05). Other metabolites measured by MRS showed no significant changes. Liver T1ρ and T2 relaxation times significantly increased three days after TAA injection compared to pre-injection. There was a correlation between Gln levels in the brain and the relaxation time of the liver. Furthermore, Gln levels and relaxation time changed depending on the TAA dose. The Gln concentration in the brain increased with the deterioration of liver function, as inferred from the prolonged relaxation time of the liver. The prolonged relaxation time of the liver corresponded with the level of Gln in the brain. Gln concentration for the alterations of brain metabolites and T1ρ relaxation time for the assessment of liver damage are useful markers for inter-organ association analysis in the HE model.
Understanding Inborn Errors of Metabolism through Metabolomics
Authors: Driesen Karen , Witters Peter
Published: April 2022

Inborn errors of metabolism (IEMs) are rare diseases caused by a defect in a single enzyme, co-factor, or transport protein. For most IEMs, no effective treatment is available and the exact disease mechanism is unknown. The application of metabolomics and, more specifically, tracer metabolomics in IEM research can help to elucidate these disease mechanisms and hence direct novel therapeutic interventions. In this review, we will describe the different approaches to metabolomics in IEM research. We will discuss the strengths and weaknesses of the different sample types that can be used (biofluids, tissues or cells from model organisms; modified cell lines; and patient fibroblasts) and when each of them is appropriate to use.
Deformation Analysis of Continuous Milling of Inconel718 Nickel-Based Superalloy
Authors: Li Xueguang , Wang Yahui , Miao Liqin , Zhang Wang
Published: April 2022

As a difficult-to-process material, Inconel718 nickel-based superalloy is more and more widely used in aerospace, ocean navigation, and large-scale machinery manufacturing. Based on ABAQUS simulation software, this paper takes the milling force and temperature in the milling process of the nickel-based superalloy as the research object, and establishes the empirical formula for the prediction model of cutting force and cutting temperature based on the method of multiple linear regression. The significance of the prediction model was verified by the residual analysis method. Through data analysis, it is obtained: within a certain experimental range, the influence degrees of each milling parameter on the cutting force and cutting temperature are fz>ap>n and fz>ap≈n, respectively. The actual orthogonal cutting test was carried out on the machine tool, and the reliability and accuracy of the prediction model of cutting force, cutting temperature and tool wear amount were verified. The model formulas of the shear velocity field, shear strain field and shear strain rate field of the main shear deformation zone are constructed by using mathematical analysis methods. The influence law of cutting speed and tool rake angle on the variables of main shear zone is calculated and analyzed. Through the combination of theory and experiment, the relationship between cutting force, chip shape and machined surface quality in milling process was analyzed. Finally, with the increase in the cutting force, the serration of the chip becomes more and more serious, and the roughness of the machined surface becomes greater and greater.
P–Ru-Complexes with a Chelate-Bridge-Switch: A Comparison of 2-Picolyl and 2-Pyridyloxy Moieties as Bridging Ligands
Authors: Ehrlich Lisa , Gericke Robert , Brendler Erica , Wagler Jörg
Published: April 2022

Starting from [Ru(pyO)2(nbd)] 1 and a N,P,N-tridentate ligand (2a: PhP(pic)2, 2b: PhP(pyO)2) (nbd = 2,5-norbornadiene, pic = 2-picolyl = 2-pyridylmethyl, pyO = 2-pyridyloxy = pyridine-2-olate), the compounds [PhP(μ-pic)2(μ-pyO)Ru(κ2-pyO)] (3a) and [PhP(μ-pyO)3Ru(κ2-pyO)] (3b), respectively, were prepared. Reaction of compounds 3 with CO and CNtBu afforded the opening of the Ru(κ2-pyO) chelate motif with the formation of compounds [PhP(μ-pic)2(μ-pyO)Ru(κ-O-pyO)(CO)] (4a), [PhP(μ-pic)2(μ-pyO)2Ru(CNtBu)] (5a), [PhP(μ-pyO)4Ru(CO)] (4b) and [PhP(μ-pyO)4Ru(CNtBu)] (5b). In dichloromethane solution, 4a underwent a reaction with the solvent, i.e., substitution of the dangling pyO ligand by chloride with the formation of [PhP(μ-pic)2(μ-pyO)Ru(Cl)(CO)] (6a). The new complexes 3a, 4a, 5a, 5b and 6a were characterized by single-crystal X-ray diffraction analyses and multi-nuclear (1H, 13C, 31P) NMR spectroscopy. The different coordination behaviors of related pairs of molecules (i.e., pairs of 3, 4 and 5), which depend on the nature of the P–Ru-bridging ligand moieties (μ-pic vs. μ-pyO), were also studied via computational analyses using QTAIM (quantum theory of atoms in molecules) and NBO (natural bond orbital) approaches, as well as the NCI (non-covalent interactions descriptor) for weak intramolecular interactions.
Nicotinic Acetylcholine Receptors and Microglia as Therapeutic and Imaging Targets in Alzheimer’s Disease
Authors: Takata Kazuyuki , Kimura Hiroyuki , Yanagisawa Daijiro , Harada Koki , Nishimura Kaneyasu , Kitamura Yoshihisa , Shimohama Shun , Tooyama Ikuo
Published: April 2022

Amyloid-β (Aβ) accumulation and tauopathy are considered the pathological hallmarks of Alzheimer’s disease (AD), but attenuation in choline signaling, including decreased nicotinic acetylcholine receptors (nAChRs), is evident in the early phase of AD. Currently, there are no drugs that can suppress the progression of AD due to a limited understanding of AD pathophysiology. For this, diagnostic methods that can assess disease progression non-invasively before the onset of AD symptoms are essential, and it would be valuable to incorporate the concept of neurotheranostics, which simultaneously enables diagnosis and treatment. The neuroprotective pathways activated by nAChRs are attractive targets as these receptors may regulate microglial-mediated neuroinflammation. Microglia exhibit both pro- and anti-inflammatory functions that could be modulated to mitigate AD pathogenesis. Currently, single-cell analysis is identifying microglial subpopulations that may have specific functions in different stages of AD pathologies. Thus, the ability to image nAChRs and microglia in AD according to the stage of the disease in the living brain may lead to the development of new diagnostic and therapeutic methods. In this review, we summarize and discuss the recent findings on the nAChRs and microglia, as well as their methods for live imaging in the context of diagnosis, prophylaxis, and therapy for AD.
Polyoxometalate/Cellulose Nanofibrils Aerogels for Highly Efficient Oxidative Desulfurization
Authors: Song Rui , Zhang Xueqin , Wang Huihui , Liu Chuanfu
Published: April 2022

Polyoxometalate (POM) presents great potential in oxidative desulfurization (ODS) reaction. However, the high dissolubility of POM in common solvents makes it difficult to recycle. Besides, the small specific surface area of POM also limits the interaction between them and the substrate. Depositing polyoxometalates onto three-dimensional (3D) network structured materials could largely expand the application of POM. Here, the surfaces of cellulose nanofibrils (CNFs) were modified with very few (3-Aminopropyl) trimethoxysilane (APTS) to endow positive charges on the surfaces of CNFs, and then phosphotungstic acid (PTA) was loaded to obtain the aerogel A-CNF/PTA as the ODS catalyst. FT-IR indicated the successful deposition of PTA onto aminosilane modified CNF surfaces. UV-VIS further suggested the stability of PTA in the aerogels. BET and SEM results suggested the increased specific surface area and the relatively uniform 3D network structure of the prepared aerogels. TGA analysis indicated that the thermal stability of the aerogel A-CNF/PTA50% was a little higher than that of the pure CNF aerogel. Most importantly, the aerogel A-CNF/PTA50% showed good catalytic performance for ODS. Catalysis results showed that the substrate conversion rate of the aerogel A-CNF/PTA50% reached 100% within 120 min at room temperature. Even after five cycles, the substrate conversion rate of the aerogel A-CNF/PTA50% still reached 91.2% during the dynamic catalytic process. This work provides a scalable and facile way to stably deposit POM onto 3D structured materials.
Synthesis and Investigation of the G-Quadruplex Binding Properties of Kynurenic Acid Derivatives with a Dihydroimidazoquinoline-3,5-dione Core
Authors: Mazzini Stefania , Princiotto Salvatore , Musso Loana , Passarella Daniele , Beretta Giovanni Luca , Perego Paola , Dallavalle Sabrina
Published: April 2022

G-quadruplexes are secondary structures originating from nucleic acid regions rich in guanines, which are well known for their involvement in gene transcription and regulation and DNA damage repair. In recent studies from our group, kynurenic acid (KYNA) derivative 1 was synthesized and found to share the structural features typical of G-quadruplex binders. Herein, structural modifications were conducted on this scaffold in order to assist the binding with a G-quadruplex, by introducing charged hydrophilic groups. The antiproliferative activity of the new analogues was evaluated on an IGROV-1 human ovarian cancer cell line, and the most active compound, compound 9, was analyzed with NMR spectrometry in order to investigate its binding mode with DNA. The results indicated that a weak, non-specific interaction was set with duplex nucleotides; on the other hand, titration in the presence of a G-quadruplex from human telomere d(TTAGGGT)4 showed a stable, although not strong, interaction at the 3′-end of the nucleotidic sequence, efficiently assisted by salt bridges between the quaternary nitrogen and the external phosphate groups. Overall, this work can be considered a platform for the development of a new class of potential G-quadruplex stabilizing molecules, confirming the crucial role of a planar system and the ability of charged nitrogen-containing groups to facilitate the binding to G-quadruplex grooves and loops.
Application of Levan-Rich Digestate Extract in the Production of Safe-to-Use and Functional Natural Body Wash Cosmetics
Authors: Wasilewski Tomasz , Seweryn Artur , Pannert Dominika , Kierul Kinga , Domżał-Kędzia Marta , Hordyjewicz-Baran Zofia , Łukaszewicz Marcin , Lewińska Agnieszka
Published: April 2022

The study focused on the evaluation of the possibility of using a levan-rich digestate extract in the production of safe and functional body wash cosmetics. Model shower gels were designed and formulated on the basis of raw materials of natural origin. Prepared prototypes contained various extract concentrations (16.7; 33; 50%). A gel without extract was used as a reference. The samples were evaluated for their safety in use and functionality. The results showed that the use of high-concentration levan-rich digestate extract in a shower gel resulted in a significant reduction in the negative impact on the skin. For example, the zein value decreased by over 50% in relation to the preparation without the extract. An over 40% reduction in the emulsifying capacity of hydrophobic substances was also demonstrated, which reduces skin dryness after the washing process. However, the presence of the extract did not significantly affect the parameters related to functionality. Overall, it was indicated that levan-rich digestate extract can be successfully used as a valuable ingredient in natural cleansing cosmetics.
Pico-Watt Scanning Thermal Microscopy for Thermal Energy Transport Investigation in Atomic Materials
Authors: Koo Seunghoe , Park Jaehee , Kim Kyeongtae
Published: April 2022

The thermophysical properties at the nanoscale are key characteristics that determine the operation of nanoscale devices. Additionally, it is important to measure and verify the thermal transfer characteristics with a few nanometer or atomic-scale resolutions, as the nanomaterial research field has expanded with respect to the development of molecular and atomic-scale devices. Scanning thermal microscopy (SThM) is a well-known method for measuring the thermal transfer phenomena with the highest spatial resolution. However, considering the rapid development of atomic materials, the development of an ultra-sensitive SThM for measuring pico-watt (pW) level heat transfer is essential. In this study, to measure molecular- and atomic-scale phenomena, a pico-watt scanning thermal microscopy (pW-SThM) equipped with a calorimeter capable of measuring heat at the pW level was developed. The heat resolution of the pW-SThM was verified through an evaluation experiment, and it was confirmed that the temperature of the metal line heater sample could be quantitatively measured by using the pW-SThM. Finally, we demonstrated that pW-SThM detects ultra-small differences of local heat transfer that may arise due to differences in van der Waals interactions between the graphene sheets in highly ordered pyrolytic graphite. The pW-SThM probe is expected to significantly contribute to the discovery of new heat and energy transfer phenomena in nanodevices and two-dimensional materials that have been inaccessible through experiments.