bluuebunny/crossref_metadata_2025 huggingface dataset:
The relationship between residual cancer burden and disease-free survival in locally advanced post-neoadjuvant chemotherapy breast cancer at Dr. Soetomo General Hospital, Surabaya
Authors: Utomo Heningtyas Suci , Suprabawati Desak Gede Agung , Ali Iskandar
Published: January 2025

Link of Video Abstract: https://youtu.be/c8EpIRNHkG0
 
Background: Locally advanced breast cancer is characterized by advanced-stage breast tumors without distant metastasis. Neoadjuvant chemotherapy is employed in locally advanced breast cancer to reduce tumor burden and enable breast surgery. Neoadjuvant chemotherapy also offers an opportunity to evaluate breast cancer patient responses to therapeutic modalities. The Residual Cancer Burden (RCB) index combines pathological findings from the primary tumor base and regional lymph nodes to calculate a continuous index that accurately predicts recurrence rates and survival in breast cancer patients. This study aimed to assess recurrence-free survival to estimate the prognostic level of breast cancer after neoadjuvant chemotherapy based on the RCB Index.
Methods: This study is an observational analytical retrospective cohort study involving patients with locally advanced breast cancer who received neoadjuvant chemotherapy-based treatment. Clinical data and RCB scores were obtained retrospectively. Local and distant recurrence were followed for two years.
Results: A total of 30 research subjects were included in this study. The RCB score was ≤1 in 10% of cases and >1 in 90% of cases. The recurrence rate for local and distant recurrence within 24 months was 50%, with a recurrence rate of 0% for RCB ≤1 and 37% for RCB >1. Chi-square analysis indicated no significant difference in the incidence of recurrence within 24 months between RCB ≤1 and RCB >1 with a p-value of (0.224).
Conclusion: There is no relationship between RCB and disease-free survival in locally advanced breast cancer following neoadjuvant chemotherapy.
Traumatic brain injury due to fish arrow impalement in rural Maluku Islands area: a case report
Authors: Ruspanah Ivanmorl , Farhandika Adria Putra , Ruspanah Andrew , Huwae Laura Bianca Sylvia , Wenno Harold Roy , Pelamonia Gracellia Astrid
Published: January 2025

Background: Traumatic brain injury (TBI) is defined as impaired brain function caused by sudden brain damage by blunt or sharp trauma that causes penetrating injuries into the brain. Globally, the annual incidence of TBI is estimated to vary between 27 and 69 million. TBI is the most frequently encountered case by neurosurgeons and is also one of the causes of disability and even death.
Case illustration: A 51-year-old woman was referred to Ambon from Tiakur Hospital, Southwest Maluku, by boat, traveling for 3 days with pain in the head after being impaled by a fish arrow. The onset of the incident when she arrived in Ambon was already 4 days. During the onset time, the patient never experienced decreased consciousness, nausea, seizures, or weakness in the limbs. On the 4th day, the patient complained of severe headaches and difficulty seeing in the left eye, and the patient tended to get emotional; however, when the patient was asked to assess the level and content of his consciousness, the patient was able to answer well (E4M6V5). The patient appears moderately ill, with a visual analog scale (VAS) of 4-5. On physical examination, a stab wound was found on the outer edge of the patient's left eye with the tip of the spear entering the patient's head, accompanied by prominent bruising on almost the entire left eyelid which was blue-black in color. This patient underwent broad-spectrum antibiotic medical therapy with ceftriaxone in combination with metronidazole, intravenous phenytoin as seizure prophylaxis, antitetanus injection, and operative craniotomy and debridement with a bifrontal craniotomy approach. The patient was sent home after 14 days of treatment with the condition improving and the patient able to carry out normal daily activities.
Conclusion: Swift assessment, prompt operations, and continuous monitoring are crucial to improving the quality of life for patients with TBI and reducing their mortality rates, especially in rural areas.
The anti-inflammatory potential of epigallocatechin gallate (EGCG) on tumor necrosis factor alpha and interleukin–10 expression in pseudomonas keratitis model (in vivo study on Rattus norvegicus rats model)
Authors: Fathimah Fariztah Sukainah Nur , Zuhria Ismi , Lutfi Delfitri , Urolita Tarosa Yodia , Karima Annisa , Kurniasari Nila , Purwanto Djoko Agus
Published: January 2025

Background: Pseudomonas aeruginosa infection is the primary cause of keratitis, leading to gradual corneal deterioration and resulting in scarring, thinning, and corneal perforation. Antibiotic treatment alone may prove ineffectual in certain instances, as the inflammatory process may remain despite the elimination of bacteria. Due to its antibacterial, anti-inflammatory, and antioxidant properties, Epigallocatechin gallate (EGCG) has the potential to be used as an adjuvant therapy for Pseudomonas keratitis. The objective of this study is to analyze the expression of TNF-α and IL-10 in a P. aeruginosa-induced keratitis model following the administration of moxifloxacin and EGCG eye drops.
Methods: Rattus norvegicus with keratitis induced by clinical isolate P. aeruginosa were randomly allocated to one of three treatment groups: the negative control group given NaCl 0.93%  and benzalkonium chloride 0.01%; the group given moxifloxacin eye drops; and the group given moxifloxacin eye drops and EGCG 50 μg/mL on the third to fifth day after keratitis induction. Immunohistochemical staining of corneal tissue was utilized to analyze the levels of TNF-α and IL-10 expression.
Results: On the fifth day of subsequent keratitis induction, a statistically significant difference in TNF-α expression was observed across the groups (p=0.015). It was found between the moxifloxacin and EGCG 50 g/mL groups and the negative control group (p=0.008). IL-10 expression showed no significant difference across the groups (p=0.108).
Conclusion: TNF-α expression was significantly different among the three groups, whereas IL-10 expression was not significantly different.
Correlation between discharge readiness and quality of discharge instructions in Chinese pediatric liver transplant parents
Authors: He ting  , Tang LI hua , Huang Xiao Bo , Siti Fatimah Sa’at
Published: January 2025

Introduction: The objective is to examine the present state of preparedness for discharge and the quality of discharge instructions provided to parents of pediatric liver transplant patients and to investigate the relationship between these two factors.
Methods: A total of 103 pediatric liver transplant patients from the Organ Transplantation Department of the First Affiliated Hospital of Guangxi Medical University were chosen as the participants for this study. The study involved administering questionnaires, including a general information questionnaire, the Chinese version of the Discharge Readiness Scale, and the Discharge Guidance Quality Scale.
Results: The overall readiness score for parents of children who underwent liver transplants was 76.91±22.01, with a mean score of 6.41±1.83. The total quality of discharge instruction for these parents was 122.56±23.56, with a mean score of 5.11±0.98. The readiness scores of these parents were positively correlated with the total score of discharge instruction quality (r=0.651, P<0.001), the content obtained (r=0.672, P<0.001), and the presentation skills and effectiveness (r=0.588, P<0.001).
Conclusion: The caliber of nurses' discharge instructions directly influences parents' preparedness for hospital release and predicts readmission risk. Consequently, enhancing the quality of nurses' discharge instructions is a paramount concern for enhancing children's health outcomes. Nurses should prioritize enhancing the quality of discharge counseling, evaluating the healthcare requirements of parents of pediatric liver transplant patients based on their current situations, and implementing tailored nursing interventions to enhance parents' preparedness for the patient's release.
The correlation between lactate levels and the length of stay in the intensive care unit in post-operative patients of rheumatic valvular heart disease surgery
Authors: Utami Sulistiyati Bayu , Sawitri Rakhma Dyah , Bayuadi Imam Suseno , Wicaksono Satrio Adi
Published: January 2025

Background: Valvular heart surgery with cardiopulmonary bypass (CPB) may be accompanied by increased serum lactate levels. Serum lactate levels can predict postoperative outcomes. This study aimed to correlate serum lactate levels to the length of stay (LOS) in the intensive care unit (ICU) in patients with rheumatic valvular heart surgery (RVHS).
Method: This was a prospective cohort study of thirty (30) patients with post-cardiac surgery of RVHS from February 2016 to June 2019 in Dr. Kariadi Hospital Semarang, Indonesia. Subjects were selected by consecutive sampling. Serum lactate levels were examined in one hour after RVHS. Patients were divided into 2 groups, i.e. group with lactate levels <3.0 mmol/L and with ≥3.0 mmol/L. Patients were followed up during hospitalization. The differences in LOS in the ICU were analyzed using the Mann-Whitney test. The correlation between serum lactate levels and the LOS in the ICU was analyzed by the Spearman correlative test.
Results: The average lactate level in the ICU was 3.9 ± 1.06 mmol/L. The average CPB time was 68.7 ± 8.55 minutes. The median of LOS in ICU was 3 days (range 2–6 days). There were no differences in baseline characteristics, echocardiographic, and laboratory data between both groups, except height (p=0.036), heart rate (p=0.025), and CPB time (57.40 ± 4.93 vs 71.00 ± 7.22, p=0.001). There were no significant differences in LOS in total in-hospital stay (p=0.813) and LOS in ICU stay ( p=0.132) between both groups. There was a significant positive moderate correlation between serum lactate levels and the LOS in the ICU (Spearman r=0.522, p=0.003).
Conclusion: There was a moderate positive correlation between serum lactate levels and the LOS in ICU patients with RVHS. If confirmed by further studies, lactate levels may be a predictor for the outcome of RVHS.
Comparative analysis of pathoscan hrHPV qPCR kits with four- and five-channel qPCR methods for the identification of high-risk human papillomaviruses
Authors: Wulandari Dewi , Umboh Rocky Dousje , Mardian Yan
Published: January 2025

Introduction: In Indonesia, cervical cancer remains a serious health concern. WHO recommends high-risk human papillomavirus (hrHPV) DNA testing as the primary tool of screening instead of cytology and visual inspection with acetic acid. The Indonesian government has been encouraging the usage of locally manufactured medical devices including laboratory supplies and reagent kits. PathoScan hrHPV qPCR kit (PT Riset Nusantara Genetika, Nusantics Group, Indonesia) is one of the locally manufactured kits and was originally designed for 5-channel qPCR for hrHPV DNA detection. However, most clinical laboratories in Indonesia employ 4-channel qPCR instruments. Therefore, the PathoScan hrHPV Lite qPCR kit was developed as an alternative, needing only a 4-channel qPCR instrument. This study aims to assess its diagnostic performance in comparison to the PathoScan hrHPV qPCR kit
Methods: This is a cross-sectional study evaluating the PathoScan hrHPV Lite qPCR kit (4-channel) on 96 cervical swab samples that were previously tested with the PathoScan hrHPV qPCR kit (5-channel) to determine its diagnostic performance using the following metrics: sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV). Bland-Altman analysis was utilized to evaluate the agreement between Ct values obtained between the two methods.
Result: The diagnostic performance of the kit was assessed on 96 cervical swab samples, which comprised 47 positive and 49 negative samples as determined by the PathoScan kit. The PathoScan hrHPV Lite qPCR kit's sensitivity in the present study was 100% (95% CI 92.29-100%) and the specificity observed was 100% (95% CI 92.75-100%). The Bland-Altman plot indicated good agreement with <5% results falling beyond the acceptable upper and lower limits.
Conclusion: PathoScan hrHPV Lite qPCR kit displayed excellent diagnosis accuracy and good agreement, suggesting it can be used for primary cervical cancer screening.
Effect of scalp nerve block with ropivacaine on interleukin-1β, critical-care pain observation tool (CPOT), and fentanyl dose in elective craniotomy patients
Authors: Karamoy Ovi Octavia , Airlangga Prananda Surya , Kriswidyatomo Prihatma , Waloejo Christijogo Soemartono , Hamzah  , Santosa Dhania Anindita , Semedi Bambang Pujo , Susila Dedi , Komarullah Noor Syamsu , Desiree Pricilia , Lestari Pudji , Pandi Moses Glorino Rumambo
Published: January 2025

Background: Considering that there is a gap in information regarding the effectiveness of Scalp Nerve Block (SNB) in post-craniotomy patients in reducing pain and inflammation, this study aims to examine the effectiveness of SNB in craniotomy patients through two variables, namely IL-1β and CPOT. In addition, this study also analyzed the dose of fentanyl in patients given SNB.
Methods: This research is a prospective experimental randomized control trial (RCT) double-blind pre-post control group design study. A total of 40 patients underwent elective craniotomy at Dr. Soetomo General Hospital during October – November 2023. IL-1β as an inflammatory parameter was measured at post-induction, 4 hours post-incision, and 24 hours post-incision. CPOT score and fentanyl dose were measured up to 24 hours post-operatively.
Results: The results of the comparison test for IL-1β levels at 4 hours post-incision in the SNB and non-SNB groups were significantly different (p<0.01). The delta levels of IL-1β post-induction and 4 hours post-incision were significantly different (p<0.05). However, there was no significant difference in IL-1β levels 24 hours post-incision or in the delta of IL-1β levels post-induction and 24 hours post-incision. In the non-SNB group, the results of the comparison test for the movement of IL-1β levels were significantly different (p<0.001). The comparison test of post-craniotomy CPOT scores in the SNB group with the non-SNB group at 4 hours and 8 hours was significantly different (p<0.05). The comparative test of fentanyl use during surgery up to 24 hours after craniotomy between the SNB group and the non-SNB group was significantly different (p<0.05).
Conclusion: SNB with ropivacaine was effective in suppressing IL-1β levels in craniotomy up to 4 hours post-incision. SNB can also reduce post-craniotomy pain by reducing the CPOT score, as well as reducing the dose of fentanyl up to 24 hours after craniotomy.
Efficacy of tenofovir treatment in chronic hepatitis B
Authors: Sungkar Taufik , Inger Hereta Sylviana , Ilhamd
Published: January 2025

Introduction: Hepatitis B virus (HBV) infection remains a global health concern, with a significant number of morbidity and mortality. Tenofovir disoproxil fumarate (TDF) has emerged as a first-line treatment for chronic HBV infection, however, its safety and efficacy in Indonesian populations remains understudied. In this study, we aim to evaluate the safety and efficacy of TDF as a chronic HBV infection treatment.
Methods: This cross-sectional study evaluated the association between the period of TDF treatment duration and the corresponding treatment response in chronic HBV patients. Data were collected through medical records sources of subjects receiving TDF treatment for at least 6 months. Efficacy was assessed based on HBV DNA viral load levels and Loss of HbeAg.
Result: A total of 60 subjects were included in this study with a mean age of 46 years, and a predominantly male population. Period of TDF treatment duration showed a significant association with HBV DNA levels, with longer treatment associated with a better treatment response. Loss of HbeAg was observed in 44.1% of study subjects, in which longer treatment durations correlated with higher rates of loss of HbeAg.
Conclusion: TDF treatment demonstrated a significant efficacy in reducing HBV DNA levels and inducing Loss of HbeAg in chronic HBV patients.
Ni-doped Al2O3-based materials for the photocatalytic degradation of phenol
Authors: Gómez-Cholula Deysi , Crespo Juan Edgar Carrera , Morales Francisco Javier Tzompantzi , Cipagauta-Díaz Sandra
Published: January 2025

The photocatalytic degradation of phenol over Ni-doped Al2O3 was investigated. Materials varying Ni concentrations were synthesized and characterized by XRD, FTIR, UV–Vis–DRS, and XPS. The results showed that Ni doping significantly enhanced photocatalytic activity, attributable to increased UV light absorption and efficient charge carrier separation. The optimal Ni concentration was found to be 1.5 wt%, achieving a 70% phenol degradation in 4 h. These findings suggest that Ni-doped Al2O3 holds great promise for wastewater remediation.

Graphical abstract
Kolonisasi mikoriza Claroideoglomus etunicatum dalam menurunkan penyakit busuk akar Rhizoctonia solani pada kacang hijau
Authors: Chasanah Laila Uswatun , Kasiamdari Rina Sri
Published: January 2025

Mung bean (Vigna radiata (L.) R. Wilczek) is widely cultivated in Indonesia, but root rot disease caused by Rhizoctonia solani can decrease production. Arbuscular mycorrhiza can be an alternative biocontrol to control root rot. This research aimed to determine the character of R. solani, analyze the growth of mung bean infected by R. solani inoculated with mycorrhizal fungi Claroideoglomus etunicatum, and determine the severity suppression of root rot disease in mung bean inoculated with mycorrhizal fungi C. etunicatum. The study used a Completely Randomized Design (CRD) with six treatments and four replications including control (without inoculation), R. solani, 120 spores of C. etunicatum, 180 spores of C. etunicatum, 120 spores of C. etunicatum + R. solani, and 180 spores of C. etunicatum + R. solani. This research showed that R. solani had white to brown colonies, cottony, septate hyphae with 90° branching, hyphae width 5.45 – 9.79 μm, multinucleate, slow growth with an abundant-aerial pattern. Inoculation of C. etunicatum on mung bean infected by R. solani had a significant effect on increasing shoot fresh weight, shoot dry weight, and pod length and C. etunicatum reduced the intensity of R. solani disease by 44.44 – 55.46% and reduced the infection of R. solani in mung bean root by 38.10 – 52.38%. In conclusion, C. etunicatum was able to suppress the severity of rot root disease caused by R. solani on mung bean.

ABSTRAK

Kacang hijau (Vigna radiata (L.) R. Wilczek) banyak dibudidayakan di Indonesia, tetapi penyakit busuk akar akibat Rhizoctonia solani menyebabkan penurunan jumlah produksi. Mikoriza arbuskular dapat menjadi alternatif biokontrol untuk mengendalikan busuk akar. Penelitian ini bertujuan untuk mengetahui karakter jamur R. solani, menganalisis pertumbuhan kacang hijau terinfeksi R. solani yang diinokulasi jamur mikoriza Claroideoglomus etunicatum, serta mengetahui penekanan keparahan penyakit busuk akar pada kacang hijau yang diinokulasi jamur mikoriza C. etunicatum. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan enam perlakuan dan empat ulangan, yaitu kontrol (tanpa inokulasi), R. solani, 120 spora C. etunicatum, 180 spora C. etunicatum, 120 spora C. etunicatum + R. solani, dan 180 spora C. etunicatum + R. solani. Penelitian ini menunjukkan bahwa R. solani memiliki koloni putih hingga coklat, cottony, hifa bersekat dengan percabangan 90°, lebar hifa 5,45 – 9,79 μm, multinukleat, pertumbuhan slow dengan pola abundant-aerial. Inokulasi C. etunicatum pada kacang hijau terinfeksi R. solani berpengaruh nyata terhadap peningkatan berat basah tajuk, berat kering tajuk, dan panjang polong serta C. etunicatum menurunkan intensitas penyakit R.solani sebesar 44,44% – 55,56% dan menurunkan infeksi R. solani pada akar kacang hijau sebesar 38,10% – 52,38%. Kesimpulan penelitian ini, C. etunicatum mampu menekan keparahan penyakit busuk akar oleh R. solani pada kacang hijau.
Respon empat varietas bawang putih (Allium sativum L.) lokal Indonesia terhadap media induksi dan proliferasi kalus embriogenik
Authors: Hafizah Rumaisha Afifatul , Sobir Sobir , Aisyah Syarifah Iis , Tamami Djoko , Roostika Ika
Published: January 2025

Establishing a regeneration media of Indonesian local garlic is necessary for several purposes, including plant breeding and large-scale propagation. This study was aimed to evaluate media formulation on callus induction and proliferation of four local garlic varieties (Geol, Lumbu Hijau, Lumbu Kuning, and Lumbu Putih) using root cuttings as the explants. MS media supplemented with different concentration of picloram (4 and 6 mg L-1) without and in combination with glutamine (100 mg L-1) alone and casein hydrolysate (3 g L-1) were evaluated. The results showed that the responses of induction and proliferation of embryogenic callus were genotype-dependent because there was no significant interaction between varieties and media formulations. Still, the varieties had a significant interaction with the observed variables. The fastest initiation time of callus induction was obtained from Lumbu Putih, less than 2 weeks after culture. Geol showed the highest percentage of callus formation and fresh weight of callus, 59% and 0,92 g respectively. There were three different types of the callus: (1) friable, glossy, clear white, (2) friable, glossy, transparent yellow, and (3) semi compact, glossy, yellowish to milky white.

ABSTRAK

Pemantapan media regenerasi bawang putih lokal Indonesia penting dilakukan untuk berbagai tujuan, termasuk pemuliaan tanaman dan perbanyakan skala besar. Penelitian ini bertujuan untuk mengetahui respon in vitro empat varietas bawang putih lokal (Geol, Lumbu Hijau, Lumbu Kuning, dan Lumbu Putih) terhadap komposisi media induksi dan proliferasi kalus dengan menggunakan akar sebagai eksplan. Komposisi media yang diujikan meliputi media dasar MS yang mengandung pikloram (4 dan 6 mg L-1), baik tanpa atau dengan penambahan glutamin (100 mg L-1) dan kasein hidrolisat (3 g L-1). Hasil penelitian menunjukkan respon induksi dan proliferasi kalus embriogenik bersifat genotype dependent, sebab tidak terdapat interaksi yang nyata antara faktor varietas dan formulasi media, namun faktor varietas berpengaruh nyata terhadap variabel amatan. Waktu inisiasi kalus tercepat diperoleh dari Lumbu Putih, yaitu kurang dari 2 minggu setelah kultur. Varietas Geol memiliki persentase pembentukan kalus dan bobot segar kalus tertinggi, berturut-turut sebesar 59% dan 0,92 g. Terdapat tiga tipe kalus yang terbentuk, yaitu (1) remah, mengkilap, putih bening, (2) remah, mengkilap, bening kekuningan, dan (3) kompak, mengkilap, kekuningan-putih susu.
Estimasi Daya Gabung Galur Jagung Manis (Zea mays saccharata Sturt) Pada Karakter Kegenjahan Dan Hasil Dengan Menggunakan Genotipe+Genotipe x Environment (GGE) Biplot
Authors: Syihab Fakhri Nasharul , Karuniawan Agung , Ismail Ade , Yuwariah Yuyun , Ruswandi Dedi
Published: January 2025

Sweet corn is an important commodity in Indonesia, but its productivity is low due to the use of seeds that have undergone genetic degradation. This research aims to estimate general combining ability (GCA) and spesific combining Ability (SCA) on maturity characteristics and sweet corn yield. The research was conducted in Cikajang Village, Garut Regency, from April to July 2023, using a randomized block design with 40 treatments repeated three times. Analysis was carried out using the Genotype + Genotype x Environment (GGE) Biplot method. The variance results showed a significant effect of line, tester, and line x tester interactions on male flowering age, female flowering age, harvest age, and yield. From the GGE Biplot analysis, the "mean vs stability" pattern identified GCA, with 9 lines having good GCA at male flowering age, 14 at female flowering age, 7 at harvest age, and 10 at yield. The "Which Won Where/What" pattern identified SCA, where the 3 best line x tester combinations were found at male flowering age, 2 at female flowering age, 3 at harvest age, and 4 at yield. The use of GGE Biplot makes it easier to estimate combining ability, so that lines with good GCA are recommended as parents, and hybrids with the best SCA are recommended as superior cultivars that produce earliness and high yields.

ABSTRAK

Jagung manis merupakan salah satu komoditas penting di Indonesia, namun produktivitasnya rendah karena penggunaan benih yang mengalami degradasi genetik. Penelitian ini bertujuan mengestimasi daya gabung umum (DGU) dan daya gabung khusus (DGK) pada karakter kegenjahan dan hasil jagung manis. Penelitian dilakukan di Desa Cikajang, Kabupaten Garut, dari April hingga Juli 2023, menggunakan rancangan acak kelompok dengan 40 perlakuan yang diulang tiga kali. Analisis dilakukan menggunakan metode Genotipe + Genotipe x Lingkungan (GGE) Biplot. Hasil varians menunjukkan pengaruh signifikan dari line, tester, serta interaksi line x tester terhadap umur berbunga jantan, umur berbunga betina, umur panen, dan hasil. Dari analisis GGE Biplot, pola "mean vs stability" mengidentifikasi DGU, dengan 9 galur memiliki DGU baik pada umur berbunga jantan, 14 pada umur berbunga betina, 7 pada umur panen, dan 10 pada hasil. Pola "Which Won Where/What" mengidentifikasi DGK, di mana 3 kombinasi line x tester terbaik ditemukan pada umur berbunga jantan, 2 pada umur berbunga betina, 3 pada umur panen, dan 4 pada hasil. Penggunaan GGE Biplot mempermudah estimasi daya gabung, sehingga galur dengan DGU baik direkomendasikan sebagai tetua, dan hibrida dengan DGK terbaik direkomendasikan sebagai kultivar unggul yang menghasilkan umur genjah dan hasil tinggi.
Corn growth on gold-mine tailings inoculated with nitrogen-fixing and phosphate-solubilizing bacteria
Authors: Sunarya Yaya - , Priyadi Rudi , Arifin Mahfud , Hindersah Reginawanti
Published: January 2025

Gold-mine tailings, challenging environment for plant growth, was our study focus. Introducing nitrogen-fixing bacteria (NFB) and phosphate-solubilizing bacteria (PSB) provides nutrients and phytohormones for plant growth. A pot experiment was designed to assess the corn growth on tailing inoculated with NFB and PSB. The research, conducted in a completely randomized block design, was replicated seven times; the treatments were : without inoculation (control), single inoculation of Azo-7.2, single inoculation of BPF-9, a mixture of Azo-7.2 and BPF-9. The results revealed that inoculation of NFB and PSB significantly increased plant height, stem diameter, leaf number, and P-uptake but did not affect leaf area, chlorophyll content, root length, S/R ratio, N-uptake, and plant biomass, and NFB and PSB count in the rhizosphere. Single inoculants of BPF-9 and mixed inoculants increased plant height by 1.2% to 7%, stem diameter, leaves number, and S/R ratio; only mixed inoculation increased N-uptake, however, Azo-7.2 potential to enhance leaf area, chlorophyll content, and corn biomass. The population of NFB and PSB in the rhizosphere of all treated and control plants was slightly lower than the initial population. The research, in particular, verified that the corn growth on tailings inoculated with NFB and PSB was better than that of uninoculated.

ABSTRAK

Tailing tambang emas yang merupakan tantangan untuk pertumbuhan tanaman, menjadi fokus penelitian ini. Inokulasi bakteri pengikat nitrogen (BPN) dan bakteri pelarut fosfat (BPF) menyediakan nutrisi dan fitohormon yang penting untuk pertumbuhan tanaman. Percobaan pot dirancang untuk mengevaluasi kinerja pertumbuhan jagung (Zea mays L.) pada tailing yang diinokulasi dengan BPN dan BPF. Penelitian dilakukan dengan rancangan acak kelompok yang diulang sebanyak tujuh kali; perlakuan percobaan adalah tanpa inokulasi (kontrol) dan dengan inokulasi tunggal BPN Azo-7.2 dan BPF-9 serta campuran Azo-7.2 dan BPF-9. Hasil penelitian menunjukkan bahwa inokulasi BPN dan BPF dengan nyata meningkatkan tinggi tanaman, diameter batang, dan jumlah daun tetapi tidak mempengaruhi luas daun, kandungan klorofil, panjang akar, biomassa tanaman, serta jumlah BPN dan BPF di rizosfer. Inokulan tunggal BPF-9 dan inokulan campuran campuran meningkatkan tinggi tanaman 1,2% sampai 7%, diameter batang, jumlah daun, dan rasio S/R secara signifikan. Namun Azo-7.2 berpotensi untuk meningkatkan luas daun, kandungan klorofil, dan biomassa jagung. Populasi BPN dan BPF di rizosfer seluruh tanaman yang diberi perlakuan dan kontrol sedikit lebih rendah dibandingkan populasi awal sebelum percobaan. Penelitian ini, secara khusus, memastikan bahwa performansi pertumbuhan jagung pada tailing yang diinokulasi dengan BPN dan BPF lebih baik dibandingkan dengan tanaman di tailing tanpa inokulasi.
Agronomic characteristics of nagara sweet potatoes (Ipomoea batatas L.) from south kalimantan wetlands
Authors: Apriani Rila Rahma , Nugroho Agung , Adriani Dewi Erika , Purnomo Joko
Published: January 2025

Understanding the agronomic characteristics of sweetpotato grown in wetland is critical to optimise cultivation and maximise yield. By studying factors such as plant morphology and yield, as well as environmental conditions, is important to identify varieties and cultivation practices that are most suitable for specific agroecological zones. This research aims to study the agronomic characters of nagara sweet potato in South Kalimantan's wetlands and identify abiotic factors that support its growth. A total of 15 sweet potato accessions were randomly sampled in sweet potato cultivation hotspot areas in July 2024. Hierarchical cluster analysis was conducted to see the similarity of the accessions found. The results showed that the agronomic characters of sweet potato accessions differed from the registered variety (Ubi Nagara KB-1), especially in characteristics type & number of leaf lobes, shape and number of tubers. Sweet potato yield was also found to be lower (14 t ha-1) compared to Ubi Nagara KB-1 (20 t ha-1). Abiotic data were found to be favourable for sweet potato agronomy, except for pH. The accessions found are still recommended to be developed in lebak swamp land, due to their adaptability and potential which is still higher than sweet potato in general (in dry land). Genetic testing is needed to prove that morphological differences are caused by different varieties (genetic) or decreased environmental conditions.

ABSTRAK

Karakteristik agronomi ubi jalar yang ditanam di lahan basah sangat penting dipelajari untuk mengoptimalkan budidaya dan memaksimalkan hasil panen. Pemahaman faktor-faktor seperti morfologi dan hasil tanaman, serta kondisi lingkungan, penting untuk mengidentifikasi varietas dan praktik budidaya yang paling sesuai untuk zona agroekologi tertentu. Penelitian ini bertujuan mempelajari karakter agronomi ubi nagara di lahan rawa lebak Kalimantan Selatan dan mengidentifikasi faktor abiotik yang mendukung pertumbuhannya. Sebanyak 15 aksesi ubi jalar diambil secara acak di area sentra budidaya ubi pada Juli 2024. Analisis kluster hierarki dilakukan untuk melihat kekerabatan aksesi yang ditemukan. Hasil penelitian menunjukkan bahwa karakter agronomi aksesi ubi jalar berbeda dengan varietas yang terdaftar (Ubi Nagara KB-1), terutama pada ciri tipe, jumlah cuping daun, bentuk dan jumlah umbi. Hasil ubi jalar juga ditemukan lebih rendah (14 t ha-1) dibandingkan dengan Ubi Nagara KB-1 (20 t ha-1). Data abiotik ditemukan mendukung agronomi ubi jalar, kecuali pH. Aksesi yang ditemukan masih direkomendasikan untuk dikembangkan di lahan rawa lebak, karena adaptabilitas dan potensinya yang masih lebih tinggi dibanding ubi jalar secara umum (di lahan kering). Uji genetik diperlukan untuk membuktikan perbedaan morfologi disebabkan oleh varietas berbeda (genetik) atau penurunan kondisi lingkungan.
Enhancing microbial population and biomass of water spinach grown in tailing and inceptisols by manure amendment
Authors: Hindersah Reginawanti , Suryatmana Pujawati , Herdiyantoro Diyan , Hamdani Jajang Sauman
Published: January 2025

The impact of tailings accumulated on agricultural land is the loss of soil profile and decreased soil quality, making plants difficult to grow. This study aimed to observe the effect of cow dung manure (CM) doses to gold mine tailings on total fungal and bacterial populations of soil surrounding roots and water spinach biomass and to analyze the correlation between fungal and bacterial populations with water spinach growth parameters. The experiment was designed in a randomized block design with five treatments and five replications. The treatments included without CM (control) and 5, 10, 15, and 20% of CM in tailing. Similar treatments were added to plants grown in mineral soil, i.e. Inceptisols. The results determined the retarded plant growth in tailing compared to that in Inceptisols. The plant grown in tailing was more responsive to manure amendment. The CM increased total fungal and bacterial populations in the soil around the roots, plant height, leaf number, stem thickness, wet weight, and dry weight of intact plants. Applying 5% of CM caused better growth of water spinach than other treatments. Total fungal and bacterial populations were strongly correlated with water spinach height and dry weight.

ABSTRAK

Dampak negatif penumpukan tailing di lahan pertanian adalah hilangnya profil tanah dan penurunan kualitas tanah sehingga tanaman sulit tumbuh. Penelitian ini bertujuan untuk mengobservasi pengaruh pemberian dosis pupuk kotoran sapi (PKS) pada tailing tambang emas terhadap populasi jamur dan bakteri total biomassa kangkung darat (Ipomoea reptans (L.) Poir.) serta menganalisis korelasi antara populasi jamur dan bakteri di tanah sekitar perakaran dengan parameter pertumbuhan kangkung. Percobaan pot di rumah kaca disusun dalam rancangan acak kelompok dengan lima perlakuan dan lima ulangan. Perlakuan percobaan adalah tanpa dan dengan penambahan 5, 10, 15 dan 20% PKS ke dalam tailing. Perlakuan yang sama diberikan pada tanaman kangkung dengan tanah Inceptisol. Hasil percobaan menunjukkan bahwa pertumbuhan kangkung di tailing terhambat dibandingkan di tanah Inceptisols, tetapi tanaman di tailing lebih responsif terhadap aplikasi PKS. Pupuk kotoran sapi mampu meningkatkan populasi jamur dan bakteri total di sekitar perakaran, tinggi tanaman, jumlah daun, ketebalan batang, bobot basah serta bobot kering tanaman di tailing. Pemberian 5% PKS lebih meningkatkan pertumbuhan tanaman kangkung dibandingkan dengan perlakuan lainnya. Populasi jamur dan bakteri masing-masing berkorelasi positif dengan hubungan yang sangat kuat dengan bobot kering serta tinggi tanaman kangkung. Percobaan ini menjelaskan bahwa bahan organik penting untuk memperbaiki kualitas tailing dan pertumbuhan tanaman.
Curriculum adaptation for learners with diverse learning needs: A case of South African inclusive rural schools
Authors: Ledwaba Raesetja Gloria , Sefotho Maximus Monaheng
Published: January 2025

In this article we report on Foundation Phase teachers’ experiences when implementing curriculum adaptation in rural inclusive schools of South Africa. We argue that, in spite of inclusive education policies being in place and several pre- and in-service training have been undertaken, teachers still experience challenges to meet the learning needs of their learners by means of curriculum adaptation. We relied on purposeful sampling to select 6 Foundation Phase teachers from 3 inclusive schools in 2 South African provinces. We followed a qualitative approach and implemented a phenomenology research design. Data were collected through individual semi-structured interviews and were audio-recorded. Following thematic data analysis, we identified themes that related to the significance of early identification of learning barriers, knowledge of curriculum adaptation, application of curriculum adaptation in practice and barriers preventing the effective implementation of curriculum adaptation by teachers. Based on the findings of the study, we recommend continuous in-service teacher development and support by district-based teams, as such efforts may improve the experiences of teachers when implementing curriculum adaptation in inclusive schools.
Digital literacy and online learning satisfaction among junior high school students: A moderated mediation model
Authors: Liu Xue-zhi , Wu Jian-xiao , Li Bing-bing , Guo Ling-jing , Ye Bao-juan
Published: January 2025

To explore the mechanism among digital literacy, online learning satisfaction, online learning engagement, and parents’ educational expectations, a survey-based quantitative research approach was adopted to collect data. A total of 916 Chinese junior high school students completed an online questionnaire that included the digital literacy scale, online learning engagement scale, perceived parental expectation (PPE) scale, and satisfaction with online learning scale. The analysis of the moderated mediation model revealed several key findings: (1) digital literacy had a positive correlation with online learning engagement and online learning satisfaction; (2) online learning engagement mediated the relationship between digital literacy and online learning satisfaction; (3) parents’ educational expectations moderated the first half path (i.e., the association between digital literacy and online learning engagement) of the mediating effect. In other words, the relationship between digital literacy and online learning satisfaction could be characterised as a moderated mediated model. More measures should be taken to improve junior high school students’ digital literacy and express parents’ educational expectations appropriately to promote the quality of online learning.
Effect of geometrical characteristics of TBM disc cutter on rock excavation: A focus on cutter diameter and cutter tip profile
Authors: Palassi Massoud , Koohmishi Mehdi , Jangi Mahmoud
Published: January 2025

Disk cutters are the most commonly employed cutting tools in various types of tunnel boring machines (TBMs). Estimating the force required for cutting a specific type of rock using disc cutters is crucial aspect of the TBM cutter head design and the prediction of TBM performance. In the present study, various types of rocks are fractured by applying load through a disc cutter near the edge of cubic rock samples using a uniaxial compressive strength test apparatus. Regarding the geometrical configuration of TBM disc cutters, different cutter diameters and various cutter tip radii are used to determine the failure load for each rock type and to develop a machine learning prediction model. The results confirm that larger disc diameters and greater tip radii increase the failure load as high as 20% and more than 100%, respectively. Meanwhile, the profile used for the cutter tip is the most prominent parameter influencing the normal force leading to failure.
Supposed Corpses and Correspondence
Authors: Sugarman Elise
Published: January 2025

The correspondence requirement is a fundamental doctrinal principle in Anglo-American criminal law.  It maintains that, in general, a particular relation between mens rea and actus reus is necessary for liability.  Yet the nature of this relation is contested.  Contemporaneity Theorists maintain that correspondence requires temporal overlap between mens rea and actus reus, while Causal Theorists maintain that correspondence is a non-deviant causal connection.  
In this paper, I argue that neither Contemporaneity Theory nor Causal Theory is able to account for the intuition that a special class of defendants—defendants in so-called supposed corpse cases—are liable for murder. Supposed corpse defendandts attempt to kill at t1, erroneously suppose they have done so, and then act again to cause death at t2.  I go on to provide a novel positive proposal of correspondence in such cases.  I argue that supposed corpse defendants are liable for murder because their killing is explained by ignorance that is in turn explained by an apparently successful execution of their intention to kill. The result serves as demonstration that the relation between intention and action grounding culpability is not the same as the relation grounding an action's status as intentional.
Rapid Single-Tube Plant DNA Isolation Suitable for PCR v1
Authors: Kafer Chris
Published: January 2025

Described is an inexpensive single-tube mini-prep method of plant DNA isolation from small amounts of starting plant tissue. Isolated DNA is suitable for PCR applications such as Marker Assisted Selection or transgene detection. Fresh tissue is first disrupted in STE buffer, centrifuged and then lysed in standard Edwards buffer to release DNA, followed by a single 2-propanol precipitation and then re-suspension in TE buffer. The protocol is relatively quick, requires no hazardous organic solvents, and also minimizes plastic waste and transfer steps. The protocol reduces co-precipitation of PCR inhibitors and is an improvement on several previously published rapid protocols. The protocol has been used successfully on tomato leaf but the protocol should be applicable to other species as well.
DNA extraction in Theridion evexum v1
Authors: Madrigal-Brenes Ruth , Barrantes Gilbert , Sandoval Luis , J. Fuchs Eric
Published: January 2025

The number of species threatened by fragmentation and loss of natural habitats due to changes in land use and unplanned urban expansion are rapidly increasing. Despite the seriousness of the situation, information on the impact of isolation caused by fragmentation and urbanization on spider genetic diversity is scarce, owing mostly to a lack of appropriate molecular markers. The main objective of this study was to develop microsatellite (SSR) primers for the spider Theridion evexum using low-coverage next-generation sequencing and bioinformatic tools. To increase the yield of DNA extracted from small spiders like T. evexum, we also optimized a CTAB DNA extraction protocol. We sequenced eight individuals at 4X using paired-end sequencing on an Illumina Novaseq 6000. Reads were cleaned and processed using the MiMi python pipeline. MiMi produced a total of 3999 putative microsatellite primers. After filtering for polymorphic loci with an allelic richness greater than three and primers that were present in at least 5 of the 8 sequenced individuals, 34 final markers were identified. An in vivo test of 13 of these 34 markers showed that 10 loci were polymorphic with at least three detectable alleles, one locus was monomorphic, and two loci did not produce PCR products. These markers will allow a better assessment of the effects of fragmentation and isolation across populations of this spider species. Furthermore, developing markers using low-coverage NGS (next-generation sequencing) and bioinformatic methods provide a valuable approach for uncovering SSR markers at a reduced cost for other tropical species, thereby broadening the scope of molecular ecology research in the tropics.
PHYTOTHERAPY USED IN AFRO-INDIGENOUS COMMUNITIES FOR ANIMALS AND PEOPLE
Authors: Cordão Maiza Araújo
Published: January 2025

Ethnobotany is the ethnographic study of plants and herbs passed down through the oral tradition of a particular ethnic group. The African Brazilian religion, Candomblé, keeps and transmits medicinal knowledge in its practices, such as the use of herbs and plants, which, in their biochemical context, are effective against various clinical pathologies. With the growth of the pharmaceutical industry, science is also expanding its research into new drugs and options for medicinal treatment, thus giving rise to herbal medicine. In addition to reducing economic costs, as it is an abundant raw material, i.e., widely present even in people's backyards, herbal medicine provides a variety of effective therapeutic options for treating pathologies. The aim of this study was to identify the medicinal herbal knowledge used in some African-Brazilian communities in João Pessoa-PB. Interviews were carried out, using a questionnaire, with babalorixás and yalorixás from the municipality. The information collected from the religious leaders revealed a wide range of knowledge, such as the use of O. basilicum (basil) to treat colic in newborn children, respiratory and stomach problems. The herbal knowledge of the African community's religious leaders is wide-ranging and applicable to the treatment of various pathologies. 100% of the priests claimed to have used herbs and plants to treat children of the house, and 80% of the priests to treat non-human animals. Some of the herbs and plants mentioned were: thorny pigweed, wild sage, skunkweed, American wormseed, Brazilian peppertree, cow-foot leaf, aloe vera, peppermint, myrrh, boldo, rosemary, and basil. The infectious diseases treated were gastrointestinal, pain, wounds, and even more complex infections such as pneumonia. It was observed that the religious leaders usevarious medicinal plants and herbs for several symptoms, from topical to oral uses, both in humans and in animals, which gives us the possibility of using this knowledge in one health medicine.
ASSISTÊNCIA DE ENFERMAGEM A CRIANÇAS COM TRANSTORNO DO ESPECTRO AUTISTA
Authors: Rodrigues Pedro Paulo , Borges Costa Caio , Moreira de Alencar Rayane , Rodrigues Pinho Paula Tatiana
Published: January 2025

O Transtorno do Espectro Autismo (TEA) é a terminologia empregada para conglomerar distintas síndromes assinaladas por característica comuns. A abordagem a crianças com TEA demanda dos profissionais de saúde habilidades, conhecimento e estratégia de cuidado individualizado. O enfermeiro possui um contato direto com a criança com autismo, onde configura-se como fundamental para o auxílio ao cuidado a criança com TEA. Esse estudo teve como objetivo analisar a assistência de enfermagem a crianças com o Transtorno do Espectro Autista. Tratou-se de uma Revisão Integrativa, realizada nos meses de agosto a dezembro de 2023, nas bases de dados LILACS, Medline e BDENF onde foram utilizados os Descritores em Ciências da Saúde (DeSC): “Criança”, “Transtorno do espectro autista” e “Cuidados de enfermagem” resultando em 08 estudos. Os resultados mostraram que o enfermeiro saber como realizar o acolhimento e cuidado de modo integral a criança autista que considere todas as questões do TEA, e não se limitando somente ao acompanhamento do crescimento e desenvolvimento. Conclui-se que devem ser desenvolvidas estratégias voltadas a assistência de enfermagem a criança autista, o que irá contribuir para a multiplicação das estratégias de saúde, fornecendo uma assistência humanizada, individualizado e resolutivo.