bluuebunny/crossref_metadata_2025 huggingface dataset:
Sirt1-PPARS Cross-Talk in Complex Metabolic Diseases and Inherited Disorders of the One Carbon Metabolism
Authors: Kosgei Viola J. , Coelho David , Guéant-Rodriguez Rosa-Maria , Guéant Jean-Louis
Published: August 2020

Sirtuin1 (Sirt1) has a NAD (+) binding domain and modulates the acetylation status of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC1α) and Fork Head Box O1 transcription factor (Foxo1) according to the nutritional status. Sirt1 is decreased in obese patients and increased in weight loss. Its decreased expression explains part of the pathomechanisms of the metabolic syndrome, diabetes mellitus type 2 (DT2), cardiovascular diseases and nonalcoholic liver disease. Sirt1 plays an important role in the differentiation of adipocytes and in insulin signaling regulated by Foxo1 and phosphatidylinositol 3′-kinase (PI3K) signaling. Its overexpression attenuates inflammation and macrophage infiltration induced by a high fat diet. Its decreased expression plays a prominent role in the heart, liver and brain of rat as manifestations of fetal programming produced by deficit in vitamin B12 and folate during pregnancy and lactation through imbalanced methylation/acetylation of PGC1α and altered expression and methylation of nuclear receptors. The decreased expression of Sirt1 produced by impaired cellular availability of vitamin B12 results from endoplasmic reticulum stress through subcellular mislocalization of ELAVL1/HuR protein that shuttles Sirt1 mRNA between the nucleus and cytoplasm. Preclinical and clinical studies of Sirt1 agonists have produced contrasted results in the treatment of the metabolic syndrome. A preclinical study has produced promising results in the treatment of inherited disorders of vitamin B12 metabolism.
Nanometre-Scale Visualization of Chemical Parameter Changes by T1-Weighted ODMR Imaging Using a Fluorescent Nanodiamond
Authors: Fujisaku Takahiro , Igarashi Ryuji , Shirakawa Masahiro
Published: August 2020

The dynamics of physical parameters in cells is strongly related to life phenomena; thus, a method to monitor and visualize them on a single-organelle scale would be useful to reveal unknown biological processes. We demonstrate real-time nanometre-scale T1-weighted imaging using a fluorescent nanodiamond. We explored optically detected magnetic resonance (ODMR) contrast at various values of interval laser pulse (τ), showing that sufficient contrast is obtained by appropriate selection of τ. By this method, we visualized nanometre-scale pH changes using a functionalized nanodiamond whose T1 has a dependence on pH conditions.
Homogeneous Nucleation of Hydroxyapatite, Ca5OH(PO4)3, at 37 °C
Authors: Madsen Hans
Published: August 2020

Precipitation of the calcium phosphate hydroxyapatite, Ca5OH(PO4)3, is studied by simple mixing of reagent solutions and measurement of light scattering (turbidimetry) at six different wavelengths from 300 to 800 nm. Measured turbidities are analyzed using Mie’s theory of light scattering from small particles. Results are interpreted in terms of classical theory of homogeneous nucleation, and from this the surface free energy of crystals is determined. The low value thus found is explained as the effect of protonation of the strongly basic anions hydroxide and phosphate at the crystal surface. Relatively large particles registered by turbidimetry are shown to be not monocrystals, but aggregates of nanocrystals.
Data Analysis of Land Use Change and Urban and Rural Impacts in Lagos State, Nigeria
Authors: O. Onilude Olalekan , Vaz Eric
Published: August 2020

This study examines land use change and impacts on urban and rural activity in Lagos State, Nigeria. To achieve this, multi-temporal land use and land cover (LULC) datasets derived from the GlobeLand30 product of years 2000 and 2010 for urban and rural areas of Lagos State were imported into ArcMap 10.6 and converted to raster files (raster thematic maps) for spatial analysis in the FRAGSTATS situated in the Patch Analyst. Thus, different landscape metrics were computed to generate statistical results. The results have shown that fragmentation of cultivated lands increased in the rural areas but decreased in the urban areas. Also, the findings display that land-use change resulted in incremental fragmentation of forest in the urban areas, and reduction in the rural areas. The fragmentation measure of diversity increased in the urban areas, while it decreased in the rural areas during the period of study. These results suggest that cultivated land fragmentation is a complex process connected with socio-economic trends at regional and local levels. In addition, this study has shown that landscape metrics can be used to understand the spatial pattern of LULC change in an urban-rural context. Finally, the outcomes of this study will help the policymakers at the three levels of governments in Nigeria to make crucial informed decisions about sustainable land use.
Correlation between Oral Hygiene and IL-6 in Children
Authors: Lo Giudice Roberto , Militi Angela , Nicita Fabiana , Bruno Giancarlo , Tamà Cristina , Lo Giudice Fabrizio , Puleio Francesco , Calapai Fabrizio , Mannucci Carmen
Published: August 2020

The aim of this study was to evaluate the correlation between marginal gingivitis, oral hygiene parameters, and interleukin-6 (IL-6) levels in gingival crevicular fluid of 40 children. The marginal periodontal pathology was evaluated by gingival index (GI). The status of oral hygiene was estimated by using patient hygiene performance (PHP), brushing frequency (BF), and plaque index (PI). IL-6 levels in gingival crevicular fluid were measured to evaluate the inflammation in marginal gingiva. PHP score showed a significant correlation with GI, BF, and PI. The groups based on PHP ranges were significantly related to IL-6 concentration in crevicular fluid.
Gintropy: Gini Index Based Generalization of Entropy
Authors: Biró Tamás S. , Néda Zoltán
Published: August 2020

Entropy is being used in physics, mathematics, informatics and in related areas to describe equilibration, dissipation, maximal probability states and optimal compression of information. The Gini index, on the other hand, is an established measure for social and economical inequalities in a society. In this paper, we explore the mathematical similarities and connections in these two quantities and introduce a new measure that is capable of connecting these two at an interesting analogy level. This supports the idea that a generalization of the Gibbs–Boltzmann–Shannon entropy, based on a transformation of the Lorenz curve, can properly serve in quantifying different aspects of complexity in socio- and econo-physics.
Agent-Based In-Vehicle Infotainment Services in Internet-of-Things Environments
Authors: Choi Dong-Kyu , Jung Joong-Hwa , Nam Hye-Been , Koh Seok-Joo
Published: August 2020

With the growth of Internet-of-Things (IoT) technology and the automobile industry, various In-Vehicle Infotainment (IVI) services have been developed, in which users can exploit a variety of IVI devices, such as navigation systems, cameras, speakers, headrest displays and heated seats. A typical IVI system is based on the peer-to-peer model, in which the user will directly control each device. This tends to induce a large overhead and inconvenience to the user. To overcome the drawbacks of the peer-to-peer model, the centralized IVI (C-IVI) scheme was recently proposed in which an IVI master is employed to provide IVI services between users and devices. However, the centralized model gives lower performance, as the number of users and devices gets larger. To improve the performance of IVI services, in this paper, we propose an agent-based IVI (A-IVI) scheme. In the proposed A-IVI scheme, a new entity called ‘agent’ is introduced, based on the C-IVI model. Each IVI agent will be used to manage a group of devices and also to perform the communication with the IVI master, on behalf of the concerned devices. The proposed scheme can be used to provide scalability and perform enhancement. The IVI agents are also helpful for supporting a variety of constrained IVI devices, such as speakers or cameras, which may usually have too low power to perform IoT communications. The proposed A-IVI scheme is implemented by using the IoT messaging protocols. For performance comparison with the existing schemes, we performed testbed experimentations. From the results, we see that the proposed A-IVI scheme can provide better performance than the existing IVI systems in terms of transmission delays, throughput and master’s loads. It is expected that the proposed scheme may be used effectively for IVI systems with a large number of users/devices, as seen in public transportation, such as public trains or airplanes.
Dynamic Modeling of a Decarbonized District Heating System with CHP Plants in Electricity-Based Mode of Operation
Authors: Koch Katharina , Alt Bastian , Gaderer Matthias
Published: August 2020

The targets of global CO2 reduction outline the importance of decarbonizing the heating and cooling sector, which consume half of the final energy in the European Union (EU). Consequently, heating network operators must adapt to growing requirements for carbon neutrality. Energy system modeling allows the simulation of individual network compositions and regulations, while considering electricity market signals for a more efficient plant operation. The district heating model, programmed for this work, covers a measured heat demand with peak load boiler, biomass-fired combined heat and power (CHP) plant, and biomass heating plant supply. The CHP plant reacts to electricity prices of the European Power Exchange market and uses a long-term heat storage to decouple heat and electricity production. This paper presents the results of three annual simulation scenarios aimed at carbon neutrality for the analyzed heating network. Two scenarios achieve a climate-neutral system by replacing the peak load boiler generation. The exclusive storage capacity expansion in the first scenario does not lead to the intended decarbonization. The second scenario increases the output of the CHP plant, while the third simulation uses the biomass heating plant supply. This additional heat producer enables a significant reduction in storage capacity and a higher CHP plant participation in the considered electricity market.
The Analysis of Working Parameters Decrease in Photovoltaic Modules as a Result of Dust Deposition
Authors: Klugmann-Radziemska Ewa , Rudnicka Małgorzata
Published: August 2020

The aspect of dust accumulation on the surface of photovoltaic (PV) modules should be thoroughly understood in order to minimize possible obstacles affecting energy generation. Several elements affect the amount of pollutant gathered on the surface of a solar device, mainly its localization, which is irreversibly linked to factors such as annual rainfall, occasional snow coverage, or, in a dry climate, increased blow of dust during sandstorms and higher concentration of soil particles in desert areas. Other than weather conditions in the region, PV installation type also plays an important role as a more horizontal position is favorable for the accumulation of soil. The research carried out and presented in this paper was done for dust accumulated naturally on PV modules kept in outdoor conditions and dust artificially sieved onto the front glass cover of modules. The experiment performed by the authors, including artificially deposited dust, defined the linear relationship between surface dust density of different types of contaminants and efficiency decline up to 10% for two different PV modules. The additional field study carried out in external conditions for a coastal region in Northern Poland concluded that, after one year, exposition photovoltaic conversion efficiency can be over 10% lower, with a slight performance improvement for the autumn season characterized by heavy rainfall.
Unstable Flow Structures Present at Different Rotational Velocities of the Centrifugal Compressor
Authors: Liśkiewicz Grzegorz , Kabalyk Kirill , Jaeschke Andrzej , Grapow Filip , Kulak Michał , Stajuda Mateusz , Kryłłowicz Władysław
Published: August 2020

Unstable flow structures cause inevitable energy losses in all power energy systems, including turbomachines. In this study, a set of analyses was conducted with the use of spectral maps on the pressure signals obtained from an industrial centrifugal compressor. The spectral maps provide one a detailed visualization of the flow conditions present in the machine along the performance curve and to distinguish the flow phenomena present prior to the surge. The method accuracy is especially useful in detecting the inlet recirculation. The study was conducted at four impeller rotational speeds with varying loads imposed by a valve at the outlet. At each speed, the machine experienced different stages of unstable flow conditions prior to the surge. Five main frequency peaks that appeared in all cases were identified and discussed. The surge was observed at all impeller speeds. At lower ones, however, it appeared at higher valve closures. At higher speeds, the surge was much more intense. The study has also shown that the inlet recirculation appears also for the closed-type industrial impeller. The phenomenon was present in all conditions. The higher impeller speed, the faster onset of the inlet recirculation was. This structure has a strong potential for an early instability warning because it appears in various types of impellers, has a very particular spectral structure and its positioning is very predictable. This study gives another example of the inlet recirculation universality and potential for efficient anti-surge protection.
Numerical Analysis of High-Altitude Inlet Air on Boundary Layer Flow Loss in an Aero-Engine Compressor
Authors: Wu Feng , Gao Limin , Yang Lu , Lin Aqiang , Zhang Hai
Published: August 2020

A numerical analysis is performed to explore the high altitude and high Mach flight on the effect of wall boundary layer loss in the compressor. The accuracy for solution results by the application of the similarity criterion and parameter definition of the air inlet is compared with the existing experimental test result. The results indicate that the radial adverse pressure gradient in the rotor domain gradually increases along the span direction and decreases as flight Mach number increases; meanwhile, the circumferential adverse pressure gradient on the pressure side of the rotor blade is correspondingly larger and less than that on the suction side. In particular, the entropy increase along the streamwise shows a decreasing trend and an increasing trend inside the hub and shroud wall boundary layers, respectively. At 2.1 Ma, the entropy increase in the rotor domains enhances by 24.36–27.80% inside the shroud boundary layer, relative to the hub boundary layer; however, it decreases by 0.97–8.54% in the stator domain. With the increase in flight Mach number from 2.1 to 3.4, the average entropy increase reductions in the rotor domain decrease by 18.99–24.97% within the hub boundary layer and 5.71–8.1% within the shroud boundary layer. In the stator domain, it drops by 18.45–9.03% inside the hub boundary layer and 6.88–8.67% inside the shroud boundary layer. It was therefore found that, as Mach number increases from 2.1 to 3.4, the entropy increase reduction is larger inside the hub boundary layer than inside the shroud boundary layer.
Impact of Water Activity on the Inactivation and Gene Expression of Listeria monocytogenes during Refrigerated Storage of Pressurized Dry-Cured Ham
Authors: Pérez-Baltar Aida , Alía Alberto , Rodríguez Alicia , Córdoba Juan José , Medina Margarita , Montiel Raquel
Published: August 2020

Listeria monocytogenes population and the expression patterns of three virulence (plcA, hly, and iap) and one stress-related (sigB) genes in dry-cured ham with different water activity (aw) values (0.92, 0.88, and 0.84) and treated with high pressure processing (HPP, 450 MPa/10 min and 600 MPa/5 min) were monitored throughout 30 days (d) at 4 °C. The antimicrobial effect of HPP at 600 MPa against L. monocytogenes S4-2 (serotype 1/2b) and S12-1 (serotype 1/2c) was greater in dry-cured ham with aw values of 0.92, with reductions of 2.5 and 2.8 log units, respectively. The efficacy of HPP treatments decreased at lower aw values. Regarding gene expression, L. monocytogenes strains responded differently to HPP. For strain S4-2, the four target genes were generally overexpressed in dry-cured ham immediately after HPP treatments at the three aw values investigated, although the extent of this induction was lower in the samples pressurized at 600 MPa and with aw values of 0.84. For strain S12-1, the expression of all target genes was repressed at the three aw values investigated. The antimicrobial efficacy of HPP against L. monocytogenes could be compromised by low aw values in food products. However, no growth of HPP-survival cells was observed during refrigerated storage in low-aw dry-cured ham, and the overexpression of virulence and stress-related genes decreased.
Mitigating Milk-Associated Bacteria through Inducing Zinc Ions Antibiofilm Activity
Authors: Hutchings Carmel , Rajasekharan Satish Kumar , Reifen Ram , Shemesh Moshe
Published: August 2020

Dairy products are a sector heavily impacted by food loss, often due to bacterial contaminations. A major source of contamination is associated with the formation of biofilms by bacterial species adopted to proliferate in milk production environment and onto the surfaces of milk processing equipment. Bacterial cells within the biofilm are characterized by increased resistance to unfavorable environmental conditions and antimicrobial agents. Members of the Bacillus genus are the most commonly found spoilage microorganisms in the dairy environment. It appears that physiological behavior of these species is somehow depended on the availability of bivalent cations in the environment. One of the important cations that may affect the bacterial physiology as well as survivability are Zn2+ ions. Thus, the aim of this study was to examine the antimicrobial effect of Zn2+ ions, intending to elucidate the potential of a zinc-based antibacterial treatment suitable for the dairy industry. The antimicrobial effect of different doses of ZnCl2 was assessed microscopically. In addition, expression of biofilm related genes was evaluated using RT-PCR. Analysis of survival rates following heat treatment was conducted in order to exemplify a possible applicative use of Zn2+ ions. Addition of zinc efficiently inhibited biofilm formation by B. subtilis and further disrupted the biofilm bundles. Expression of matrix related genes was found to be notably downregulated. Microscopic evaluation showed that cell elongation was withheld when cells were grown in the presence of zinc. Finally, B. cereus and B. subtilis cells were more susceptible to heat treatment after being exposed to Zn2+ ions. It is believed that an anti-biofilm activity, expressed in downregulation of genes involved in construction of the extracellular matrix, would account for the higher sensitivity of bacteria during heat pasteurization. Consequently, we suggest that Zn2+ ions can be of used as an effective antimicrobial treatment in various applications in the dairy industry, targeting both biofilms and vegetative bacterial cells.
Putting the Spring back into the Hare (Pedetes capensis): Meat Chemical Composition of an Underutilized Protein Source
Authors: Erasmus Sara Wilhelmina , Hoffman Louwrens Christiaan
Published: August 2020

Alternative protein sources are gaining increasing global attention as a solution to address future protein demands. Determining the chemical composition of meat alternatives is vital to confirm that it is nutritious, but also to increase product value and promote its utilization. The carcass characteristics and chemical composition of springhare, an underutilized protein source, was found to be comparable to that of commercially reared rabbits. Hence, its introduction into the commercial supply chain would likely not offset consumers accustomed to purchasing rabbit/hare meat. Springhare meat had a high protein content (~22.5 g/100 g meat) and low lipid (<1.3 g/100 g meat) content. The meat’s fatty acids mainly comprised C18:2n6c (γ-linoleic acid; 24%), C18:0 (stearic acid; 20%), C16:0 (palmitic acid; 19%), C20:4n6 (arachidonic acid; 15%) and C18:1n9c (oleic acid; 13%). Although sex did not significantly influence the carcass characteristics and meat composition, season did have an effect (p < 0.05) on the fatty acid profile. The meat harvested in summer had higher (p < 0.05) concentrations of favorable unsaturated fatty acids, C18:2n6c, C18:3n6, C18:3n3 (α-linolenic acid), C20:2n6 (eicosadienoic acid), C20:3n3 (eicosatrienoic acid), compared to the meat obtained in winter, which contained more (p < 0.05) saturated fatty acids. The results verify that springhare can be utilized as a viable alternative protein source.
Analysis of Fragmentation of Rock Blocks from Real-Scale Tests
Authors: Ruiz-Carulla Roger , Corominas Jordi , Gili Josep A. , Matas Gerard , Lantada Nieves , Moya Jose , Prades Albert , Núñez-Andrés M. A. , Buill Felipe , Puig Carol
Published: August 2020

Real-scale fragmentation tests provide high quality data in order to study the fragmentation pattern of rock blocks. In the tests carried out, the initial rock mass, in terms of both volume and shape, was reconstructed by means of 3D photogrammetry. The fragments size distribution of the bocks tested was measured by hand using a tape. The drop tests were performed in four different sites, releasing a total of 124 blocks and measuring 2907 fragments. The obtained fragment size distributions may be well fitted using power laws. The survival rate (Sr), which is the proportion of remaining block shows a wide range of values. Observing the fragment distribution, two parameters are needed to characterize the fragmentation: the number of fragments produced and Sr. The intensity of the fragmentation is expressed by the exponent of the fitted power laws. Although the results are highly variable and show a stochastic behavior of the fragmentation, we have identified different patterns that reflect some local test conditions.
Level, Source, and Spatial Distribution of Potentially Toxic Elements in Agricultural Soil of Typical Mining Areas in Xiangjiang River Basin, Hunan Province
Authors: Yu Yang , Luo Haijiang , He Lihuan , Liu Wenqing , Xu Renji , Zhang Linlin , Dong Guihua , Wang Yeyao , Wu Guoping , Wei Fusheng
Published: August 2020

The concentrations, chemical availability, distribution, and sources of potentially toxic elements (PTEs) in the soil of Xiangjiang Basin in Hunan Province, China were investigated at 85 sites. The highest mean concentrations of Cd, Cu, Zn, As, and Pb were observed in Hengyang, whereas those for Mn, Co, and Hg were observed in Changde. The pollution index values followed the order: Cd > Hg > Cu > Zn > As > Pb; the mean geo-accumulation index values were in the order: Cd > Hg > Pb > Cu > Zn > As > Co > Mn. Cd was associated with moderate contaminated level, Hg and Pb were associated with moderate contaminated to uncontaminated level, and Cu, Zn, As, Co, and Mn were associated with uncontaminated level of pollution. Furthermore, 64.5% of Cd was water-soluble and exhibited exchangeable fractions; its chemical availability posed a risk to the ecosystem. Spatial analysis, principal component analysis, and a positive matrix factorization model were used to assess the PTE sources. Four principal components contributed to 88.8% of the 8 PTEs concentrations. Mining, smelting, industrial, and agricultural activities, alongside sewage irrigation, the use of agrochemicals, and vehicular emissions are the possible anthropogenic sources that pollute agricultural products and threaten human health in the Xiangjiang Basin.
Effects of a Physical Activity Program Potentiated with ICTs on the Formation and Dissolution of Friendship Networks of Children in a Middle-Income Country
Authors: Guerra Ana M. , Montes Felipe , Useche Andrés F. , Jaramillo Ana María , González Silvia A. , Meisel Jose D. , Obando Catalina , Cardozo Valentina , Hunter Ruth F. , Sarmiento Olga L.
Published: August 2020

This paper assesses the potential cohesion effect of a physical activity (PA) school-based intervention potentiated using text messages (SMS) through analyzing longitudinally the friendship network structure and the mechanisms of the formation and dissolution of friendships. Three schools (n = 125 participants) in Bogotá, Colombia, were randomly assigned into three groups: Modulo Activo Recreo Activo (MARA) + SMS (networks 1 and 2), MARA (networks 3 and 4), and control (no intervention: networks 5–7). We collected socio-economic, health-related, network structure, and intervention satisfaction variables in the baseline and after 10 weeks on July–November 2013. For each classroom network, we conducted four models using a temporal and static network approach to assess (1) temporal social network changes, (2) friendship homophily, (3) friendship formation and dissolution mechanisms, and (4) effect of SMS on the networks’ cohesion. We found that (1) social cohesion emerged in the four intervened networks that were measured over time with transitivity and homophily driven by clustering, (2) the intervention affected the mechanisms of friendship formation and dissolution, and (3) MARA + SMS on average created more social cohesion and 3.8 more friendships than the program alone. Potentially, school-based interventions with information and communication technologies (ICT) such as MARA + SMS could encourage social cohesion among children. The particular characteristics of each school network need to be considered when developing school-based interventions.
Reply to Schade G. Comment on Hess et al. “Assessing Agreement in Exposure Classifications between Proximity-Based Metrics and Air Monitoring Data in Epidemiology Studies of Unconventional Resource Development.”
Authors: Wendt Hess Judy , Bachler Gerald , Momin Fayaz , Sexton Krystal
Published: August 2020

We appreciate the comments by Dr [...]
Parental Involvement in Children’s Sleep Care and Nocturnal Awakenings in Infants and Toddlers
Authors: Ragni Benedetta , De Stasio Simona
Published: August 2020

Background: Sleep regulation and consolidation represent critical developmental processes that occur in the first years of life. Recent studies have highlighted the contribution of caregivers to sleep development. However, the majority of them have primarily focused on maternal behaviors, overlooking fathers. The main goal of the present study was to investigate the associations between paternal and maternal involvement in children’s sleep care and the number of night awakenings reported by both parents in infants and toddlers. Methods: One-hundred-and-one families of infants aged 8 to 12 months and 54 families of toddlers aged 18 to 36 months filled out the following self-report questionnaires: The Brief Infant Sleep Questionnaire and an ad hoc questionnaire to assess parental involvement in sleep care for children. A moderate actor–partner interdependence (APIM) with path analysis was performed to test the predictive role of parental involvement on the children’s sleep (no. of nocturnal awakenings) and the moderation role of age on these relationships. Results: Paternal involvement in children’s sleep care was associated with the number of night awakenings reported by both parents. Moreover, a significant interaction effect emerged between the children’s age and paternal involvement in children’s sleep care for predicting nocturnal awakenings. Conclusions: The main outcomes of this study point to the protective role of paternal involvement in children’s sleep during the first years of life.
Opposite Effects of Work-Related Physical Activity and Leisure-Time Physical Activity on the Risk of Diabetes in Korean Adults
Authors: Oh Hyun Sook
Published: August 2020

The object of this study was to examine the effects of domestic and work-related physical activity (DWPA) and leisure-time physical activity (LTPA) on the risk of diabetes, by categorizing fasting blood glucose (FBG) levels into normal, Impaired Fasting Glucose (IFG), and diabetes. The sample consisted of 4661 adults aged 30 years or above, and was chosen from the 2017 Korean National Health and Nutrition Examination Survey (KNHANES) data. Of all the subjects, 14.6% engaged in high-intensity DWPA and 6.25% in moderate-intensity DWPA; while 11.68% and 24.80% engaged in high- and moderate-intensity LTPA, respectively. The effects of both types of physical activities on the risk of diabetes were analyzed using a Bayesian ordered probit model. For those with high-intensity DWPA, the probability of the FBG level being normal was 5.10% (SE = 0.25) lower than for those with non-high-intensity DWPA, and the probabilities of IFG and diabetes were 3.30% (SE = 0.15) and 1.79% (SE = 0.09) higher, respectively. However, for those with high-intensity LTPA, the probability of the FBG level being normal was 2.54% (SE = 0.09) higher, and the probabilities of IFG and diabetes were 1.74% (SE = 0.07) and 0.80% (SE = 0.03) lower, respectively, than those with non-high-intensity LTPA. Likewise, for moderate-intensity DWPA and LTPA, the results were the same compared to low-intensity physical activities though the magnitude of the effects were smaller than for high-intensity. Thus, the activities related to work have a negative effect and those related to leisure have a positive effect. The criteria for physical activities to reduce the risk of diabetes should be set by separating these domains of physical activity, and new management strategies for diabetes are needed for people with moderate- or high-intensity DWPA.
Context Matters: Findings from a Qualitative Study Exploring Service and Place Factors Influencing the Recruitment and Retention of Allied Health Professionals in Rural Australian Public Health Services
Authors: Cosgrave Catherine
Published: August 2020

Chronic health workforce shortages significantly contribute to unmet health care needs in rural and remote communities. Of particular and growing concern are shortages of allied health professionals (AHPs). This study explored the contextual factors impacting the recruitment and retention of AHPs in rural Australia. A qualitative approach using a constructivist-interpretivist methodology was taken. Semi-structured interviews (n = 74) with executive staff, allied health (AH) managers and newly recruited AHPs working in two rural public health services in Victoria, Australia were conducted. Data was coded and categorised inductively and analysed thematically. The findings suggest that to support a stable and sustainable AH workforce, rural public sector health services need to be more efficient, strategic and visionary. This means ensuring that policies and procedures are equitable and accessible, processes are effective, and action is taken to develop local programs, opportunities and supports that allow AH staff to thrive and grow in place at all grade levels and life stages. This study reinforces the need for a whole-of-community approach to effectively support individual AH workers and their family members in adjusting to a new place and developing a sense of belonging in place. The recommendations arising from this study are likely to have utility for other high-income countries, particularly in guiding AH recruitment and retention strategies in rural public sector health services. Recommendations relating to community/place will likely benefit broader rural health workforce initiatives.
Prenatal Fine Particulate Matter (PM2.5) Exposure and Pregnancy Outcomes—Analysis of Term Pregnancies in Poland
Authors: Wojtyla Cezary , Zielinska Karolina , Wojtyla-Buciora Paulina , Panek Grzegorz
Published: August 2020

Air pollution is currently one of the greatest threats to global health. Polish cities are among the most heavily polluted in Europe. Due to air pollution 43,100 people die prematurely in Poland every year. However, these data do not take into account the health consequences of air pollution for unborn children. Thus, the aim of this study was to evaluate the effects of the fine particulate matter air pollution (less than 2.5 μm in diameter) on pregnancy outcomes. An analysis of pregnant women and their children was made using a questionnaire survey from a nationwide study conducted in 2017. Questionnaires from 1095 pregnant women and data from their medical records were collected. An analysis of air pollution in Poland was conducted using the air quality database maintained by the Chief Inspectorate for Environmental Protection in Poland. A higher concentration of PM2.5 was associated with a decrease in birth weight and a higher risk of low birthweight (i.e., <2500 g). We also observed lower APGAR scores. Thus, all possible efforts to reduce air pollution are critically needed.
Non-Typeable Haemophilus influenzae Invade Choroid Plexus Epithelial Cells in a Polar Fashion
Authors: Wegele Christian , Stump-Guthier Carolin , Moroniak Selina , Weiss Christel , Rohde Manfred , Ishikawa Hiroshi , Schroten Horst , Schwerk Christian , Karremann Michael , Borkowski Julia
Published: August 2020

Non-typeable Haemophilus influenzae (NTHI) is a pathogen of the human respiratory tract causing the majority of invasive H. influenzae infections. Severe invasive infections such as septicemia and meningitis occur rarely, but the lack of a protecting vaccine and the increasing antibiotic resistance of NTHI impede treatment and emphasize its relevance as a potential meningitis causing pathogen. Meningitis results from pathogens crossing blood–brain barriers and invading the immune privileged central nervous system (CNS). In this study, we addressed the potential of NTHI to enter the brain by invading cells of the choroid plexus (CP) prior to meningeal inflammation to enlighten NTHI pathophysiological mechanisms. A cell culture model of human CP epithelial cells, which form the blood–cerebrospinal fluid barrier (BCSFB) in vivo, was used to analyze adhesion and invasion by immunofluorescence and electron microscopy. NTHI invade CP cells in vitro in a polar fashion from the blood-facing side. Furthermore, NTHI invasion rates are increased compared to encapsulated HiB and HiF strains. Fimbriae occurrence attenuated adhesion and invasion. Thus, our findings underline the role of the BCSFB as a potential entry port for NTHI into the brain and provide strong evidence for a function of the CP during NTHI invasion into the CNS during the course of meningitis.
Intratumoral Adipocyte-High Breast Cancer Enrich for Metastatic and Inflammation-Related Pathways but Associated with Less Cancer Cell Proliferation
Authors: Tokumaru Yoshihisa , Oshi Masanori , Katsuta Eriko , Yan Li , Huang Jing Li , Nagahashi Masayuki , Matsuhashi Nobuhisa , Futamura Manabu , Yoshida Kazuhiro , Takabe Kazuaki
Published: August 2020

Cancer-associated adipocytes are known to cause inflammation, leading to cancer progression and metastasis. The clinicopathological and transcriptomic data from 2256 patients with breast cancer were obtained based on three cohorts: The Cancer Genome Atlas (TCGA), GSE25066, and a study by Yau et al. For the current study, we defined the adipocyte, which is calculated by utilizing a computational algorithm, xCell, as “intratumoral adipocyte”. These intratumoral adipocytes appropriately reflected mature adipocytes in a bulk tumor. The amount of intratumoral adipocytes demonstrated no relationship with survival. Intratumoral adipocyte-high tumors significantly enriched for metastasis and inflammation-related gene sets and are associated with a favorable tumor immune microenvironment, especially in the ER+/HER2- subtype. On the other hand, intratumoral adipocyte-low tumors significantly enriched for cell cycle and cell proliferation-related gene sets. Correspondingly, intratumoral adipocyte-low tumors are associated with advanced pathological grades and inversely correlated with MKI67 expression. In conclusion, a high amount of intratumoral adipocytes in breast cancer was associated with inflammation, metastatic pathways, cancer stemness, and favorable tumor immune microenvironment. However, a low amount of adipocytes was associated with a highly proliferative tumor in ER-positive breast cancer. This cancer biology may explain the reason why patient survival did not differ by the amount of adipocytes.
Integrated Framework of the Immune-Defense Transcriptional Signatures in the Arabidopsis Shoot Apical Meristem
Authors: Naseem Muhammad , Osmanoğlu Özge , Kaltdorf Martin , Alblooshi Afnan Ali M. A. , Iqbal Jibran , Howari Fares M. , Srivastava Mugdha , Dandekar Thomas
Published: August 2020

The growing tips of plants grow sterile; therefore, disease-free plants can be generated from them. How plants safeguard growing apices from pathogen infection is still a mystery. The shoot apical meristem (SAM) is one of the three stem cells niches that give rise to the above ground plant organs. This is very well explored; however, how signaling networks orchestrate immune responses against pathogen infections in the SAM remains unclear. To reconstruct a transcriptional framework of the differentially expressed genes (DEGs) pertaining to various SAM cellular populations, we acquired large-scale transcriptome datasets from the public repository Gene Expression Omnibus (GEO). We identify here distinct sets of genes for various SAM cellular populations that are enriched in immune functions, such as immune defense, pathogen infection, biotic stress, and response to salicylic acid and jasmonic acid and their biosynthetic pathways in the SAM. We further linked those immune genes to their respective proteins and identify interactions among them by mapping a transcriptome-guided SAM-interactome. Furthermore, we compared stem-cells regulated transcriptome with innate immune responses in plants showing transcriptional separation among their DEGs in Arabidopsis. Besides unleashing a repertoire of immune-related genes in the SAM, our analysis provides a SAM-interactome that will help the community in designing functional experiments to study the specific defense dynamics of the SAM-cellular populations. Moreover, our study promotes the essence of large-scale omics data re-analysis, allowing a fresh look at the SAM-cellular transcriptome repurposing data-sets for new questions.
Comparing Gene Expression in the Parabrachial and Amygdala of Diestrus and Proestrus Female Rats after Orofacial Varicella Zoster Injection
Authors: Hornung Rebecca , Pritchard Addison , Kinchington Paul R. , Kramer Phillip R.
Published: August 2020

The orofacial pain pathway projects to the parabrachial and amygdala, and sex steroids have been shown to affect neuronal activity in these regions. GABA positive cells in the amygdala are influenced by sex steroid metabolites to affect pain, and sex steroids have been shown to alter the expression of genes in the parabrachial, changing neuronal excitability. Mechanisms by which sex steroids affect amygdala and parabrachial signaling are unclear. The expression of genes in the parabrachial and amygdala in diestrus (low estradiol) and proestrus (high estradiol) female rats were evaluated in this study. First, varicella zoster virus was injected into the whisker pad of female rats to induce a pain response. Second, gene expression was quantitated using RNA-seq one week after injection. Genes that had the greatest change in expression and known to function in pain signaling were selected for the quantitation of protein content. Protein expression of four genes in the parabrachial and seven genes in the amygdala were quantitated by ELISA. In the parabrachial, neurexin 3 (Nrnx3) was elevated at proestrus. Nrnx3 has a role in AMPA receptor and GABA signaling. Neuronatin (Nnat) and protein phosphatase, Mg2+/Mn2+ dependent 1E (Ppm1e) were elevated in the parabrachial of diestrus animals both genes having a role in pain signaling. Epoxide hydroxylase (Ephx2) was elevated in the parabrachial at proestrus and the vitamin D receptor (Vdr) was elevated in the amygdala. Ephx2 antagonists and vitamin D have been used to treat neuropathic pain. In conclusion, sex steroids regulate genes in the parabrachial and amygdala that might result in the greater pain response observed during diestrus.
Alterations of Rice (Oryza sativa L.) DNA Methylation Patterns Associated with Gene Expression in Response to Rice Black Streaked Dwarf Virus
Authors: Li Linying , He Yuqing , Zhang Xueying , Zhang Hehong , Sun Zongtao , Li Junmin , Hong Gaojie
Published: August 2020

Rice black-streaked dwarf virus (RBSDV) causes severe yield losses in rice (Oryza sativa L.) in China. Studies have shown that the mechanisms of DNA methylation-mediated plant defense against DNA viruses and RNA viruses are different. However, in rice its function in response to infection of RBSDV, a double-stranded RNA virus, remains unclear. In this study, high-throughput single-base resolution bisulfite sequencing (BS-Seq) was carried out to analyze the distribution pattern and characteristics of cytosine methylation in RBSDV-infected rice. Widespread differences were identified in CG and non-CG contexts between the RBSDV-infected and RBSDV-free rice. We identified a large number of differentially methylated regions (DMRs) along the genome of RBSDV-infected rice. Additionally, the transcriptome sequencing analysis obtained 1119 differentially expressed genes (DEGs). Correlation analysis of DMRs-related genes (DMGs) and DEGs filtered 102 genes with positive correlation and 71 genes with negative correlation between methylation level at promoter regions and gene expression. Key genes associated with maintaining DNA methylation in rice were analyzed by RT-qPCR and indicated that OsDMT702 might be responsible for the global increase of DNA methylation level in rice under RBSDV stress. Our results suggest important roles of rice DNA methylation in response to RBSDV and provide potential target genes for rice antiviral immunity.
Copy Number Variants Contributing to Combined Pituitary Hormone Deficiency
Authors: Budny Bartłomiej , Karmelita-Katulska Katarzyna , Stajgis Marek , Żemojtel Tomasz , Ruchała Marek , Ziemnicka Katarzyna
Published: August 2020

Combined pituitary hormone deficiency represents a disorder with complex etiology. For many patients, causes of the disease remain unexplained, despite usage of advanced genetic testing. Although major and common transcription factors were identified two decades ago, we still struggle with identification of rare inborn factors contributing to pituitary function. In this report, we follow up genomic screening of CPHD patient cohort that were previously tested for changes in a coding sequences of genes with the use of the whole exome. We aimed to find contribution of rare copy number variations (CNVs). As a result, we identified genomic imbalances in 7 regions among 12 CPHD patients. Five out of seven regions showed copy gains whereas two presented losses of genomic fragment. Three regions with detected gains encompassed known CPHD genes namely LHX4, HESX1, and OTX2. Among new CPHD loci, the most interesting seem to be the region covering SIX3 gene, that is abundantly expressed in developing brain, and together with HESX1 contributes to pituitary organogenesis as it was evidenced before in functional studies. In conclusion, with the use of broadened genomic approach we identified copy number imbalances for 12 CPHD patients. Although further functional studies are required in order to estimate its true impact on expression pattern during pituitary organogenesis and CPHD etiology.
Epigenetic Mechanisms of Inflammasome Regulation
Authors: Poli Giulia , Fabi Consuelo , Bellet Marina Maria , Costantini Claudio , Nunziangeli Luisa , Romani Luigina , Brancorsini Stefano
Published: August 2020

The innate immune system represents the host’s first-line defense against pathogens, dead cells or environmental factors. One of the most important inflammatory pathways is represented by the activation of the NOD-like receptor (NLR) protein family. Some NLRs induce the assembly of large caspase-1-activating complexes called inflammasomes. Different types of inflammasomes have been identified that can respond to distinct bacterial, viral or fungal infections; sterile cell damage or other stressors, such as metabolic imbalances. Epigenetic regulation has been recently suggested to provide a complementary mechanism to control inflammasome activity. This regulation can be exerted through at least three main mechanisms, including CpG DNA methylation, histones post-translational modifications and noncoding RNA expression. The repression or promotion of expression of different inflammasomes (NLRP1, NLRP2, NLRP3, NLRP4, NLRP6, NLRP7, NLRP12 and AIM2) through epigenetic mechanisms determines the development of pathologies with variable severity. For example, our team recently explored the role of microRNAs (miRNAs) targeting and modulating the components of the inflammasome as potential biomarkers in bladder cancer and during therapy. This suggests that the epigenetic control of inflammasome-related genes could represent a potential target for further investigations of molecular mechanisms regulating inflammatory pathways.
The Importance of Breast Adipose Tissue in Breast Cancer
Authors: Kothari Charu , Diorio Caroline , Durocher Francine
Published: August 2020

Adipose tissue is a complex endocrine organ, with a role in obesity and cancer. Adipose tissue is generally linked to excessive body fat, and it is well known that the female breast is rich in adipose tissue. Hence, one can wonder: what is the role of adipose tissue in the breast and why is it required? Adipose tissue as an organ consists of adipocytes, an extracellular matrix (ECM) and immune cells, with a significant role in the dynamics of breast changes throughout the life span of a female breast from puberty, pregnancy, lactation and involution. In this review, we will discuss the importance of breast adipose tissue in breast development and its involvement in breast changes happening during pregnancy, lactation and involution. We will focus on understanding the biology of breast adipose tissue, with an overview on its involvement in the various steps of breast cancer development and progression. The interaction between the breast adipose tissue surrounding cancer cells and vice-versa modifies the tumor microenvironment in favor of cancer. Understanding this mutual interaction and the role of breast adipose tissue in the tumor microenvironment could potentially raise the possibility of overcoming breast adipose tissue mediated resistance to therapies and finding novel candidates to target breast cancer.
Thymus Inception: Molecular Network in the Early Stages of Thymus Organogenesis
Authors: Figueiredo Marta , Zilhão Rita , Neves Hélia
Published: August 2020

The thymus generates central immune tolerance by producing self-restricted and self-tolerant T-cells as a result of interactions between the developing thymocytes and the stromal microenvironment, mainly formed by the thymic epithelial cells. The thymic epithelium derives from the endoderm of the pharyngeal pouches, embryonic structures that rely on environmental cues from the surrounding mesenchyme for its development. Here, we review the most recent advances in our understanding of the molecular mechanisms involved in early thymic organogenesis at stages preceding the expression of the transcription factor Foxn1, the early marker of thymic epithelial cells identity. Foxn1-independent developmental stages, such as the specification of the pharyngeal endoderm, patterning of the pouches, and thymus fate commitment are discussed, with a special focus on epithelial–mesenchymal interactions.