bluuebunny/crossref_metadata_2025 huggingface dataset:
Understanding Regulatory Changes: Deep Learning in Sustainable Finance and Banking
Authors: Anghel Bogdan Ionut , Lupu Radu
Published: July 2024

This paper examines the regulatory impact on the European Banking Sector using advanced deep learning techniques to analyze the relationship between Sustainable Finance guidelines and the SX7P Index from January 2012 to December 2023. Utilizing Long Short-Term Memory Auto-encoder (LSTM-AE), Variational Autoencoder (VAE), and Convolutional Neural Network (CNN) for anomaly detection, the study compares anomalies and investigates their correlation with European Banking Authority (EBA) events and Sustainable Finance guidelines from January 2020 to December 2023. Through the analysis of 43 pertinent EBA documents, the research identifies patterns and variations in anomalies, assessing their association with regulatory changes. The results reveal significant anomalies aligning with regulatory events, indicating a potential causal relationship. Notably, the VAE methodology shows the strongest correlation between EBA Sustainable Finance events and anomalies. This research advances the understanding of deep learning applications in financial markets and offers valuable insights for policymakers and financial institutions regarding regulatory shifts in Sustainable Finance.
The Veterinarian’s Role in Biocontainment Research Animal Facilities and Prevention of Spread of Pathogens: A Case of Nigeria and South Africa
Authors: Chipangura John K. , Abdussamad Abdussamad M. , Lewis David I.
Published: July 2024

Infections acquired in research laboratories and unintentional pathogen escapes from breaches in biocontainment pose risks to humans and the environment, necessitating the need for effective biosafety and biosecurity management frameworks in biocontainment research animal facilities (BRAFs). We examine key biosafety issues associated with BRAFs, including inadequate decontamination procedures for wastewater and experimental samples, handling high biosafety level pathogens in lower-level laboratories, risks of animal bites and sharps injuries, contamination of bedding and enrichment materials, and improper management and transportation of biohazard samples. Additionally, we discuss the role of veterinarians in research animal facilities and the challenges they encounter in maintaining biocontainment standards. We emphasise the importance of routine monitoring of effluent water to detect possible disease outbreaks. We recommend a thorough investigation of disease outbreaks to identify potential sources of pathogen release from BRAFs, which could serve as hotspots for future disease outbreaks. Findings from such investigations will inform the development of policies aimed at safeguarding human populations from future pandemics and preventing BRAFs from becoming sources of infectious disease outbreaks.
Sustainable Urban Landscapes in Hot–Dry Regions: Climate-Adaptive Courtyards
Authors: Ucer Hatice Busra , Tzortzi Julia Nerantzia , Lux Maria Stella , Ogut Ozge
Published: July 2024

In hot and arid environments, courtyards are essential architectural elements that significantly contribute to microclimate regulation and enhanced thermal comfort. Beyond providing protection against environmental severities, these spaces elevate the standards of livability and sustainability in urban design. The traditional landscape of Mardin, Turkey, exemplifying such challenges, takes center stage in this study, where courtyards hold a prominent role in architectural composition. Facilitated by the ENVI-met software, the evaluation process herein comprehensively analyzes four representative courtyard case studies in Mardin. Key parameters, including air temperature, humidity, predicted mean vote (PMV), and wind speed, are considered to gain a nuanced understanding of their thermal dynamics. The initial evaluation of existing conditions reveals varying thermal comfort levels, with higher PMV values indicating discomfort in the courtyards, underscoring the need for interventions to enhance their microclimate regulation and resilience to climate change challenges. This study aims to enhance our comprehension of the relationship between courtyards and microclimate regulation, particularly in hot–dry regions. By examining the design principles and passive strategies of courtyards, this research identifies effective approaches for optimizing courtyard design, aiming to create sustainable and comfortable living environments.
Enhancing Lubrication Performance of Plastic Oil Lubricant with Oleic Acid-Functionalized Graphene Nanoplatelets and Hexagonal Boron Nitride Solid Lubricant Additives
Authors: Sikdar Soumya , Menezes Pradeep L.
Published: July 2024

The study explored the viability of using waste plastic oil (PO) as an alternative lubricant to petroleum-based lubricants in industrial settings. To enhance the lubrication performance of the PO, this study incorporated cost-efficient, oleic acid-modified, graphene nano platelets [GNP (f)] and hexagonal boron nitride [hBN (f)] nano solid lubricant additives into the PO in various concentrations, forming functionalized nano lubricants. The PO and its functionalized nano lubricant’s rheological, dispersion stability, thermal degradation, friction, and wear performance were investigated. Results manifest that incorporating GNP (f) and hBN (f) into the PO significantly enhanced the viscosity and dispersion stability. In addition, it was seen that GNP (f) and hBN (f) nano lubricants lowered the coefficient of friction (COF) by 53% and 63.63% respectively, compared to the PO. However, the GNP (f) and hBN (f) nano lubricants demonstrated a 3.16% decrease and a 50.08% increase in wear volume relative to the PO. Overall, the GNP (f) and hBN (f) nano lubricants displayed a synergistic friction behavior, while they exhibited an antagonistic behavior pertaining to the wear volume. The study elucidated the mechanisms underlying friction and wear performance of the nano lubricants.
An Optimized Strain-Compensated Arrhenius Constitutive Model of GH4169 Superalloy Based on Hot Compression
Authors: Cheng Xiang , Wang Ruomin , Chen Xiaolu , Jin Shasha , Qian Qinke , Wu He
Published: July 2024

A precise constitutive model is essential for capturing the deformation characteristics of the GH4169 superalloy in numerical simulations of thermal plastic forming processes. Hence, the aim of this study was to develop a precise modified constitutive model to describe the hot deformation behavior exhibited by the GH4169 superalloy. The isothermal cylindrical uniaxial compression tests of the GH4169 superalloy were carried out at temperatures of 950~1100 °C and strain rates of 0.01~10 s−1 using a Thermecmastor-200KN thermal–mechanical simulator. The original strain–stress curves were corrected by minimizing the effects of plastic heat and interfacial friction. Based on the true stress–strain curves, the original strain-compensated Arrhenius constitutive model was constructed using polynomial orders of 3, 5, and 10, respectively. The results showed that once the polynomial order exceeds the 5th, further increasing the order has little contribution to the accuracy of the model. To improve prediction ability, a higher precision Arrhenius constitutive model was established by extending a series of material parameters as functions that depend on temperature, strain, and strain rate, in which the error can be reduced from 4.767% to 0.901% compared with the classic strain-compensated Arrhenius constitutive model.
Physico-Chemical and Biological Features of Fluorine-Substituted Hydroxyapatite Suspensions
Authors: Ciobanu Carmen Steluta , Predoi Daniela , Iconaru Simona Liliana , Predoi Mihai Valentin , Rokosz Krzysztof , Raaen Steinar , Negrila Catalin Constantin , Buton Nicolas , Ghegoiu Liliana , Badea Monica Luminita
Published: July 2024

Infections related to orthopedic/stomatology surgery are widely recognized as a significant health concern. Therefore, the development of new materials with superior biological properties and good stability could represent a valuable alternative to the classical treatments. In this paper, the fluorine-substituted hydroxyapatite (FHAp) suspension, with the chemical formula Ca10(PO4)6(OH)2−2xF2x (where x = 0.05), was prepared using a modified coprecipitation technique. Stability studies were conducted by zeta potential and ultrasound measurements for the first time. The X-ray diffraction (XRD) patterns of FHAp powders displayed a hexagonal structure akin to that of pure hydroxyapatite (HAp). The XPS general spectrum revealed peaks corresponding to the constituent elements of fluorine-substituted hydroxyapatite such as calcium, phosphorus, oxygen, and fluorine. The purity of the obtained FHAp samples was confirmed by energy-dispersive X-ray spectroscopy (EDS) studies. The FHAp morphology was evaluated by scanning electron microscopy (SEM) measurements. Fourier-transform infrared spectroscopy (FTIR) studies were performed in order to study the vibrational properties of the FHAp samples. The FHAp suspensions were tested for antibacterial activity against reference strains such as Staphylococcus aureus 25923 ATCC, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231. Additionally, the biocompatibility of the FHAp suspensions was assessed using human fetal osteoblastic cells (hFOB 1.19 cell line). The results of our biological tests suggest that FHAp suspensions are promising candidates for the future development of new biocompatible and antimicrobial agents for use in the biomedical field.
Investigation of Physical-Mechanical Properties and Microstructure of Mortars with Perlite and Thermal-Treated Materials
Authors: Saca Nastasia , Radu Lidia , Stoleriu Stefania , Dobre Daniela , Calotă Răzvan , Truşcă Roxana
Published: July 2024

This study aimed to obtain and characterize a mortar with perlite aggregate and thermal-treated materials that could substitute for Portland cement. First, the thermally treated materials were obtained by calcinating old Portland cement (OC-tt) and concrete demolition waste (CC-tt) at 550 °C, for 3 h. Second, plastic mortars with a perlite: cement volume ratio of 3:1 were prepared and tested for water absorption, mechanical strength, and thermal conductivity. The microstructure was also analyzed. Portland cement (R) was partially substituted with 10%, 30%, and 50% OC-tt. Thermal-treated materials negatively influenced the compressive and flexural strengths at 7 and 28 days. With an increase in the substitution percentage to 50%, the decrease in the compressive strength was 40% for OC-tt and 62.5% for CC-tt. The presence of 10% OC-tt/CC-tt positively influenced the water absorption. The thermal conductivity of the tested mortars was in the range of 0.37–0.48 W/m·K. SEM analysis shows the expanded perlite pores remained unbroken.
An Ultra-Wideband Frequency Selective Rasorber with Low Infrared Emissivity
Authors: Song Hang , Zhang Yuning , Zhang Shengjun , Li Jingfeng , Ai Xia , Zhang Han , Liu Jiaqi
Published: July 2024

The paper proposes an ultra-wideband frequency selective rasorber (FSR) with low infrared emissivity for the composite detection threat of both radars and infrared sensors. Firstly, the equivalent circuit (EC) method based on transmission line (TL) theory is utilized to analyze the absorption/transmission conditions. Then, based on the analysis above, sinusoidal microstrip lines with non-frequency-varying characteristics are adopted in the design, which significantly enhances the transmission bandwidth of FSR. The FSR demonstrates an absorption band ranging from 2.65 GHz to 8.80 GHz and a transmission band ranging from 9.15 GHz to 17.71 GHz. Furthermore, an infrared shielding layer (IRSL) exhibiting low emissivity in the infrared band and high transmittance in the microwave band is applied to the FSR. The simulation and experiment results verify that the IRSL-FSR demonstrates an ultra-wide transmission band ranging from 9.16 GHz to 17.94 GHz and an ultra-wide absorption band ranging from 2.66 GHz to 8.01 GHz. Additionally, it exhibits a low emissivity value (0.23) in 8–14 μm, providing a viable solution to the formidable challenge of radar-infrared bistealth for satellites and other communication-enabled flying platforms.
On the Pentanomial Power Mapping Classification of 8-bit to 8-bit S-Boxes
Authors: Dimitrov Miroslav , Baicheva Tsonka
Published: July 2024

Substitution boxes, or S-boxes, are one of the most important mathematical primitives in modern symmetric cryptographic algorithms. Given their importance, in the past decades, they have been thoroughly analyzed and evaluated by the academic world. Thus, a lot of desirable characteristics a given S-box should possess have been found. This includes, as much as possible, higher nonlinearity and algebraic degrees as well as, as much as possible, lower values of differential uniformity, autocorrelation and sum of squares indicator values. In this work, we use power mappings over GF(28) to generate, enumerate and evaluate all bijective S-boxes yielded by pentanomials of the form f(x)=xa+xb+xc+xd+xe given 0<a<b<c<d<e<256. We find a total of 152,320 different bijective S-boxes, which are further classified into 41,458 different groups in terms of the aforementioned characteristics as well as the number of their fixed points. Having this data, an S-box designer can easily generate a bijective substitution S-box with parameters of their choice. By using pentanomials, we show how we can easily construct S-boxes with cryptographic properties similar to those found in some popular S-boxes like the Kuznyechik S-box proposed by the Russian Federation’s standardization agency as well as the Skipjack S-box proposed by the National Security Agency of the USA.
Exact Solutions for the Sharma–Tasso–Olver Equation via the Sardar Subequation Method with a Comparison between Atangana Space–Time Beta-Derivatives and Classical Derivatives
Authors: Pleumpreedaporn Chanidaporn , Moore Elvin J. , Sirisubtawee Sekson , Khansai Nattawut , Pleumpreedaporn Songkran
Published: July 2024

The Sharma–Tasso–Olver (STO) equation is a nonlinear, double-dispersive, partial differential equation that is physically important because it provides insights into the behavior of nonlinear waves and solitons in various physical areas, including fluid dynamics, optical fibers, and plasma physics. In this paper, the STO equation is generalized to a fractional equation by using Atangana (or Atangana–Baleanu) fractional space and time beta-derivatives since they have been found to be useful as a model for a variety of traveling-wave phenomena. Exact solutions are obtained for the integer-order and fractional-order equations by using the Sardar subequation method and an appropriate traveling-wave transformation. The exact solutions are obtained in terms of generalized trigonometric and hyperbolic functions. The exact solutions are derived for the integer-order STO and for a range of values of fractional orders. Numerical solutions are also obtained for a range of parameter values for both the fractional and integer orders to show some of the types of solutions that can occur. As examples, the solutions are obtained showing the physical behavior, such as the solitary wave solutions of the singular kink-type and periodic wave solutions. The results show that the Sardar subequation method provides a straightforward and efficient method for deriving new exact solutions for fractional nonlinear partial differential equations of the STO type.
Generalizations of Rolle’s Theorem
Authors: Fiorenza Alberto , Fiorenza Renato
Published: July 2024

The classical Rolle’s theorem establishes the existence of (at least) one zero of the derivative of a continuous one-variable function on a compact interval in the real line, which attains the same value at the extremes, and it is differentiable in the interior of the interval. In this paper, we generalize the statement in four ways. First, we provide a version for functions whose domain is in a locally convex topological Hausdorff vector space, which can possibly be infinite-dimensional. Then, we deal with the functions defined in a real interval: we consider the case of unbounded intervals, the case of functions endowed with a weak derivative, and, finally, we consider the case of distributions over an open interval in the real line.
Research on Smart Contract Verification and Generation Method Based on BPMN
Authors: Jin Jun , Yan Le , Zou Yidan , Li Jie , Yu Zhen
Published: July 2024

The post-deployment challenges in developing and upgrading blockchain smart contracts necessitate a high level of accuracy in their development and business logic. However, current methodologies for verifying the business logic of smart contracts frequently fail to address their alignment with end-user business requirements. This paper introduces a two-step language transformation process to bridge this gap. Initially, we establish a transformation rule from the Business Process Model and Notation (BPMN) to Prolog, enabling the translation of business processes into a Prolog representation. This step not only validates the business process logic but also ensures it meets user specifications. Subsequently, we introduce a transformation rule from the BPMN to Go, which facilitates the transformation of the BPMN model, once validated, into a Go language smart contract. To enhance usability, we have engineered a dedicated tool that streamlines this transformation process. We present a case study involving a banking loan process to exemplify the utility of our tool in creating BPMN diagrams, conducting requirement and syntax validations, and effecting the transformation to Go smart contracts. The case study and empirical results suggest that our methodology and the accompanying tool mitigate the complexities inherent in smart contract development. They also ensure the fidelity of business logic to user demands, thereby promoting the broader adoption of blockchain smart contract technology.
Endothelin-1 Plasma and Aqueous Humor Levels in Different Types of Glaucoma: A Systematic Review and Meta-Analysis
Authors: Lampsas Stamatios , Kandarakis Stylianos A. , Vakalopoulos Dionysios G. , Lampsa Aikaterini , Oikonomou Evangelos , Siasos Gerasimos , Kymionis George D.
Published: July 2024

Background and Objectives: Several studies suggest the complex relationship between Endothelin-1 (ET-1) levels with various types of glaucoma. This systematic review and meta-analysis explore ET-1 levels in plasma and aqueous humor among different types of glaucoma. Materials and Methods: A literature search (PubMed, ScienceDirect, Cochrane Library) was made up to April 2024 (PROSPERO: CRD42023430471). The results were synthesized according to PRISMA Guidelines. Results were presented as standardized mean differences (SMD) with 95% confidence intervals (CI). Results: A total of 2597 subjects (1513 patients with glaucoma vs. 1084 healthy controls) from 23 studies were included in a meta-analysis. Notably, patients with glaucoma reported significantly higher plasma levels of ET-1 compared to controls (SMD: 1.21, 95% CI: 0.59–1.82, p < 0.001). Particularly, plasma ET-1 levels were higher in primary open-angle glaucoma (POAG) (SMD: 0.87, 95% CI: 0.09–1.65, p < 0.05), normal-tension glaucoma (SMD: 0.86, 95% CI: 0.27–1.46, p = 0.05), and angle-closure glaucoma patients (SMD: 1.03, 95% CI: 0.43–1.63, p < 0.001) compared to healthy controls. Moreover, ET-1 aqueous humor levels were significantly higher in patients with glaucoma compared to controls (SMD: 1.60, 95% CI: 1.04–2.15, p < 0.001). In particular, aqueous humor levels were higher in POAG patients (SMD: 2.03 95% CI: 1.00–3.14, p < 0.001), and pseudoexfoliative glaucoma patients (SMD: 2.03, 95% CI: 1.00–3.07, p < 0.001) compared to controls. Conclusions: This meta-analysis indicates that elevated levels of ET-1 plasma and aqueous humor are significantly associated with different types of glaucoma. The pathogenesis of ET-1-related mechanisms may vary across different glaucoma types, indicating that possible therapeutic approaches targeting ET-1 pathways should be tailored to each specific glaucoma type.
Leptin, Ghrelin, Irisin, Asprosin and Subfatin Changes in Obese Women: Effect of Exercise and Different Nutrition Types
Authors: Bengin Elif , Kırtepe Abdurrahman , Çınar Vedat , Akbulut Taner , Russo Luca , Aydemir İsa , Yücedal Polat , Aydın Süleyman , Migliaccio Gian Mario
Published: July 2024

Background and Objectives: In this study, the effects of a six-week training program and various diets on subfatin, asprosin, irisin, leptin, ghrelin and the lipid profile were investigated in overweight women. Materials and Methods: A total of 78 women voluntarily participated in the study. Groups: The study was divided into eight groups: Healthy Control, Obese Control, Obese + Vegetarian, Obese + Ketogenic, Obese + Intermittent Fasting, Obese + Exercise + Vegetarian, Obese + Exercise + Ketogenic and Obese + Exercise + Intermittent Fasting. While there was no intervention in the healthy and obese control groups, the other groups followed predetermined exercise and diet programs for 6 weeks. Blood samples were taken from the participants in the research group twice (before and after the interventions). An autoanalyzer was used to determine the lipid profile in the blood samples taken, and the ELISA method was used to analyze other parameters. Results: Overall, a significant difference was found in the values of weight, BMI, subfatin, ghrelin, leptin, cholesterol, triglyceride, HDL and LDL as a result of the exercise and diet interventions (p < 0.05). There was no significant difference in asprosin and irisin values (p > 0.05). Conclusions: In conclusion, regular exercise and dietary interventions in obese women can regulate lipid profile, ghrelin, leptin and asprosin levels, and increasing irisin with exercise can activate lipid metabolism and support positive changes in lean mass.
Impact of Varying Dietary Calcium Contents on the Gut Metabolomics of Yunnan Semi-Fine Wool Sheep (Ovis aries)
Authors: Khan Muhammad , Zhao Xiaoqi , Ni Xiaojun , Ali Sikandar , Danzeng Baiji , Yang Hongyuan , Mushtaq Maida , Liang Jiachong , Xue Bai , Quan Guobo
Published: July 2024

Yunnan semi-fine wool (YSFW) is a recently developed dual-purpose (meat and wool) sheep breed mainly found in Yunnan Province, China. Moreover, dietary calcium is essential for animal health and productivity. The current experiment aimed to investigate the impact of dietary calcium on sheep gut metabolite profile. For this, thirty YSFW rams (male, age = 10 months, and body weight = 40.37 ± 0.49 kg) were randomized into three groups (n = 10 rams/group), followed by a completely randomized design, and the groups were allotted to one of three dietary calcium levels (Q_1 = 0.50%, Q_3 = 0.73%, and Q_5 = 0.98% on a dry basis). The rams were fed ad libitum by feeding twice a day (at 08:00 and 17:00 h/day) throughout the experimental period (44 day). On the 21st day of the experiment, fecal samples were collected from 27 rams (9/group) and untargeted metabolite profiling was performed by using ultra-performance liquid chromatography. The PCA plot showed that the Q_5 group metabolites were clustered more tightly than for Q_1 and Q_3, respectively. The tightly clustering molecules were mainly alkaloids and their derivatives, benzenoids, lignans and related compounds, lipids, nucleotides, organic acids, and nitrogenous-based derivatives. According to the Kyoto Encyclopedia of Genes and Genomes pathway analysis, these molecules potentially contribute to metabolic pathways, biosynthesis of secondary metabolites, proteinaceous compounds, and the metabolism of the protein derivatives, particularly amino acids. The PLS-DA plots revealed a significant difference between the Q_1, Q_3, and Q_5 groups, suggesting that Q_5 had a clear separation across the groups. Based on the metabolomic analysis, feeding different levels of dietary calcium significantly changed the metabolomic profile of YSFW rams, which primarily entails metabolic pathways such as energy, protein, and lipid metabolism.
Systematic Evaluation of Biotic and Abiotic Factors in Antifungal Microorganism Screening
Authors: Gupta Gunjan , Labrie Steve , Filteau Marie
Published: July 2024

Microorganisms have significant potential to control fungal contamination in various foods. However, the identification of strains that exhibit robust antifungal activity poses challenges due to highly context-dependent responses. Therefore, to fully exploit the potential of isolates as antifungal agents, it is crucial to systematically evaluate them in a variety of biotic and abiotic contexts. Here, we present an adaptable and scalable method using a robotic platform to study the properties of 1022 isolates obtained from maple sap. We tested the antifungal activity of isolates alone or in pairs on M17 + lactose (LM17), plate count agar (PCA), and sucrose–allantoin (SALN) culture media against Kluyveromyces lactis, Candida boidinii, and Saccharomyces cerevisiae. Microorganisms exhibited less often antifungal activity on SALN and PCA than LM17, suggesting that the latter is a better screening medium. We also analyzed the results of ecological interactions between pairs. Isolates that showed consistent competitive behaviors were more likely to show antifungal activity than expected by chance. However, co-culture rarely improved antifungal activity. In fact, an interaction-mediated suppression of activity was more prevalent in our dataset. These findings highlight the importance of incorporating both biotic and abiotic factors into systematic screening designs for the bioprospection of microorganisms with environmentally robust antifungal activity.
Serological Screening and Risk Factors Associated with Leishmania infantum Positivity in Newly Diagnosed HIV Patients in Greece
Authors: Voyiatzaki Chrysa , Zare Chormizi Apollon Dareios , Tsoumani Maria E. , Efstathiou Antonia , Konstantinidis Konstantinos , Chrysos Georgios , Argyraki Aikaterini , Papastamopoulos Vasileios , Papageorgiou Effie G. , Kotsianopoulou Marika
Published: July 2024

A serological screening was conducted to detect IgG antibodies against Leishmania infantum (L. infantum) in newly diagnosed human immunodeficiency virus (HIV) patients in Greece. The study also examined potential risk factors and the agreement of commercially available serological methods. IgG antibodies against L. infantum were detected using enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence antibody test (IFAT), and Western blot (WB). Out of 155 samples, 14 (9.0%) tested positive for IgG antibodies against L. infantum using at least two methods. Statistical analysis showed substantial agreement between WB and IFAT methods (Cohen’s kappa = 0.75) but moderate overall agreement among the three methods (Fleiss’ kappa = 0.42). Additionally, HIV+ intravenous drug users faced 3.55 times (p = 0.025) higher risk of testing positive for L. infantum IgG, positing that anthroponotic transmission between these patients is a plausible hypothesis based on existing literature. Non-invasive and cost-effective techniques are preferred to detect asymptomatic infections, and leishmaniasis screening should be conducted immediately after HIV diagnosis in endemic regions to enable prophylactic treatment for leishmaniasis in addition to antiretroviral therapy. To maximize sensitivity, performing at least two different serological methods for each patient is recommended.
Age-Dependent Effects of Yolkin on Contact Sensitivity and Immune Phenotypes in Juvenile Mice
Authors: Zimecki Michał , Artym Jolanta , Kocięba Maja , Zaczyńska Ewa , Sysak Angelika , Szczypka Marianna , Lis Magdalena , Pawlak Aleksandra , Obmińska-Mrukowicz Bożena , Kaleta-Kuratewicz Katarzyna , Zambrowicz Aleksandra , Bobak Łukasz
Published: July 2024

Yolkin, an egg yolk immunoregulatory protein, stimulates the humoral but inhibits the cellular immune response in adult mice. The aim of this investigation was to evaluate the effects of yolkin administration on the immune response using a model of juvenile, i.e., 28-day- and 37-day-old, mice. We examined the yolkin influence on the magnitude of the cellular immune response, which was determined as contact sensitivity (CS) to oxazolone (OXA), and the humoral immune response, which was determined as the antibody response to ovalbumin (OVA). Yolkin was administered in drinking water, followed by immunization with OXA or OVA. In parallel, the phenotypic changes in the lymphoid organs were determined following yolkin treatment and prior immunization. The results showed that yolkin had a stimulatory effect on CS in the mice treated with yolkin from the 37th day of life but not from the 28th day of life. In contrast, no regulatory effect of yolkin on antibody production was found in 28-day- and 37-day-old mice. Phenotypic studies revealed significant changes in the content of B cells and T cell subpopulations, including CD4+CD25+Foxp3 regulatory T cells. The association between the effects of yolkin on the magnitude of CS and phenotypic changes in main T- and B-cell compartments, as well the importance of changes in T-regulatory and CD8+ cells in the age categories, are discussed. We conclude that the immunoregulatory effects of yolkin on the generation of CS in mice are age dependent and change from stimulation in juvenile to suppression in adult mice.
Sensitive Detection of Genotoxic Substances in Complex Food Matrices by Multiparametric High-Content Analysis
Authors: Gao Pengxia , Li Zhi , Gong Mengqiang , Ma Bo , Xu Hua , Wang Lili , Xie Jianwei
Published: July 2024

Genotoxic substances widely exist in the environment and the food supply, posing serious health risks due to their potential to induce DNA damage and cancer. Traditional genotoxicity assays, while valuable, are limited by insufficient sensitivity, specificity, and efficiency, particularly when applied to complex food matrices. This study introduces a multiparametric high-content analysis (HCA) for the detection of genotoxic substances in complex food matrices. The developed assay measures three genotoxic biomarkers, including γ-H2AX, p-H3, and RAD51, which enhances the sensitivity and accuracy of genotoxicity screening. Moreover, the assay effectively distinguishes genotoxic compounds with different modes of action, which not only offers a more comprehensive assessment of DNA damage and the cellular response to genotoxic stress but also provides new insights into the exploration of genotoxicity mechanisms. Notably, the five tested food matrices, including coffee, tea, pak choi, spinach, and tomato, were found not to interfere with the detection of these biomarkers under proper dilution ratios, validating the robustness and reliability of the assay for the screening of genotoxic compounds in the food industry. The integration of multiple biomarkers with HCA provides an efficient method for detecting and assessing genotoxic substances in the food supply, with potential applications in toxicology research and food safety.
The Ninhydrin Reaction Revisited: Optimisation and Application for Quantification of Free Amino Acids
Authors: Stauß Amelie Charlotte , Fuchs Carolin , Jansen Paulina , Repert Sarah , Alcock Kimberley , Ludewig Sandra , Rozhon Wilfried
Published: July 2024

The ninhydrin reaction is commonly used for the detection of amino acids. However, in the literature, different conditions with respect to the buffer system, its pH and concentration, type of organic solvent, incubation time, and temperature, as well as the concentrations of the reagents, are described. To identify the most suitable conditions, colour development with reagents of varying compositions and different reaction temperatures and times were investigated using asparagine as a model amino acid. Asparagine was selected since it is one of the most abundant free amino acids in many types of samples. The optimal reaction mixture consisted of 0.8 mol L−1 potassium acetate, 1.6 mol L−1 acetic acid, 20 mg mL−1 ninhydrin and 0.8 mg mL−1 hydrindantin in DMSO/acetate buffer 40/60 (v/v) (final concentrations). The best reaction condition was heating the samples in 1.5 mL reaction tubes to 90 °C for 45 min. Afterwards, the samples were diluted with 2-propanol/water 50/50 (v/v) and the absorbance was measured at 570 nm. The proteinogenic amino acids showed a similar response except for cysteine and proline. The method was highly sensitive and showed excellent linearity as well as intra-day and inter-day reproducibility.
Evaluation of Different Drying Treatments with Respect to Essential Oil Components, Phenolic and Flavonoid Compounds, and Antioxidant Capacity of Ajowan (Trachyspermum ammi L.)
Authors: Sobatinasab Zahra , Rahimmalek Mehdi , Etemadi Nematollah , Szumny Antoni
Published: July 2024

Ajowan (Trachyspermum ammi L.) is considered a valuable spice and medicinal herb. In this study, the essential oil content and composition of the aerial parts of ajowan were investigated under different drying treatments (sun, shade, oven at 45 °C, oven at 65 °C, microwave, and freeze drying). Moreover, the phenolic content, flavonoid content, and antioxidant capacity of samples were also assessed. Fresh samples produced the highest essential oil content (1.05%), followed by those treated under sun (0.7%) and shade drying (0.95%). Based on gas chromatography–mass spectrometry (GC–MS), thirty compounds were determined in which thymol (34.84–83.1%), carvacrol (0.15–32.36%), p-cymene (0.09–13.66%), and γ-terpinene (3.12–22.58%) were the most abundant. Among the drying methods, freeze drying revealed the highest thymol content, followed by drying in a 45 °C oven. The highest TPC (total phenolic content) and TFC (total flavonoid content) were obtained in the fresh sample (38.23 mg TAE g−1 dry weight (DW)) and in the sample oven-dried at 45 °C (7.3 mg QE g−1 DW), respectively. Based on the HPLC results, caffeic acid (18.04–21.32 mg/100 gDW) and ferulic acid (13.102–19.436 mg/100 g DW) were the most abundant phenolic acids, while among flavonoids, rutin constituted the highest amount (10.26–19.88 mg/100 gDW). Overall, freeze drying was the most promising method of drying for preserving the phenolic (TPC) and flavonoid (TFC) compounds and oil components.
Protocol for the Development and Initial Validation of the COG-IMPACT Tool: A Purpose-Built Unmet Needs Assessment for Cancer-Related Cognitive Impairment
Authors: Haywood Darren , O’Connor Moira , Baughman Frank D. , Chan Alexandre , Chan Raymond J. , Dauer Evan , Dhillon Haryana M. , Henneghan Ashley M. , Lawrence Blake J. , Lustberg Maryam , Vardy Janette L. , Rossell Susan L. , Hart Nicolas H.
Published: July 2024

(1) Background: A significant proportion of cancer survivors report experiencing a cognitive ‘fog’ that affects their ability to think coherently and quickly, and reason with clarity. This has been referred to as cancer-related cognitive impairment (CRCI). CRCI has extensive impacts on the daily lives of people living with or beyond cancer, including occupational, social, and psychological functioning. Oncology health professionals report feeling under-resourced to effectively assess the needs of an individual with CRCI and then provide optimal care and referral. (2) Methods: The objective of this project is to develop and provide an initial validation of the first purpose-built unmet needs assessment for CRCI: the Unmet Needs Assessment of Cancer-Related Cognitive Impairment Impact (COG-IMPACT). We will use a multiple-stage, co-design, mixed-methods approach to develop and provide an initial validation of the COG-IMPACT. (3) Results: The primary anticipated result of this research is the production of the COG-IMPACT, the first purpose-built unmet needs assessment for CRCI. The assessment could be used by health professionals to understand the unmet needs and facilitate optimal care and referral for cancer survivors, by survivors to elucidate their supportive needs and advocate for their care, and by researchers to examine the correlates of unmet needs relating to CRCI, as well as how best to support people with CRCI.
A Virtual Reality Direct-Manipulation Tool for Posing and Animation of Digital Human Bodies: An Evaluation of Creativity Support
Authors: Benbelkheir Youssef , Lerga Alvaro , Ardaiz Oscar
Published: July 2024

Creating body poses and animations is a critical task for digital content creators, movement artists, and sports professionals. Traditional desktop-based tools for generating 3D poses and animations often lack intuitiveness and are challenging to master. Virtual reality (VR) offers a solution through more intuitive direct-manipulation capabilities. We designed and implemented a VR tool that enables direct manipulation of virtual body parts with inverse kinematics. This tool allows users to pose and animate virtual bodies with one- or two-handed manipulations, while also moving, including bending, jumping, or walking. Our user study demonstrated that participants could produce creative poses and animations using this tool, which we evaluated for creativity support across six factors. Additionally, we discuss further opportunities to enhance creativity support.
The Economic Burden of Severe Acute Malnutrition with Complications: A Cost Analysis for Inpatient Children Aged 6 to 59 Months in Northern Senegal
Authors: Wassonguema Bibata , N’Diaye Dieynaba S. , Michel Morgane , Ngabirano Laure , Frison Severine , Ba Matar , Siroma Françoise , Brizuela Antonio V. , Audibert Martine , Chevreul Karine
Published: July 2024

Severe acute malnutrition (SAM) is a high-fatality condition that affected 13.7 million children under five years of age worldwide in 2022, with complicated cases requiring extensive inpatient stay with an accompanying caregiver. Our objective was to assess the costs of inpatient treatment for complicated SAM in children aged 6 to 59 months in Northern Senegal and identify cost predictors. We performed a retrospective cost analysis, including 140 children hospitalized from January to December 2020 in five SAM inpatient treatment facilities. We adopted a societal perspective, including direct medical and non-medical costs and indirect costs. We extracted patients’ sociodemographic and clinical data from medical records and conducted semi-structured interviews with healthcare staff to capture information on time allocation and care management. A multivariable generalized linear model with gamma family and a log link was used to investigate the factors associated with direct costs. Costs are expressed in 2020 international USD using purchasing power parity. Mean length of stay was 5.3 (SD = 3.2) days and diarrhoea was the cause of the admission in 55.7% of cases. Mean total cost was USD 431.9 (SD = 203.9), with personnel being the largest cost item (33% of the total). Households’ out-of-pocket expenses represented 45.3% of total costs and amounted to USD 195.6 (SD = 103.6). Costs were significantly associated with gender (20.3% lower in boys), diarrhoea (27% increase), anaemia (49.4% increase), inpatient death (44.9% decrease), and type of facility (26% higher in hospitals vs. health centre). Our study highlights the financial burden of complicated SAM in Senegal in particular for families. This underscores the need for tailored prevention and social policies to protect families from the disease’s financial burden and improve treatment adherence, both in Senegal and similar contexts.
Effects of Nasal Solution Incorporating Resveratrol and Carboxymethyl-Β-Glucan in Preschool Non-Atopic Children with Wheezing
Authors: Indolfi Cristiana , Mignini Costanza , Valitutti Francesco , Bizzarri Ilaria , Dinardo Giulio , Klain Angela , Miraglia del Giudice Michele , Di Cara Giuseppe
Published: July 2024

Upper respiratory tract infections (URTI) account for more than 80% of wheezing episodes in children with a high incidence of hospitalization in preschool age. Most children with symptoms of wheezing during an URTI are usually non-atopic. As the majority of wheezing episodes resulting from URTI are attributed to viral triggers, several studies have suggested the potential anti-inflammatory and antiviral properties of resveratrol. This study aims to identify the effect of resveratrol for pediatric non-atopic patients with recurrent wheezing triggered by URTIs. We conducted a prospective single-blind study to assess the effectiveness of a short course of nasal solutions incorporating resveratrol and carboxymethyl-β-glucan, administered for 7 days at the onset of URTIs, compared to standard nasal lavage with 0.9% saline solution. A total of 19 patients entered the active group, 20 patients were assigned to the placebo group. The comparison of overall wheezing days (p < 0.001), mean wheezing days per month (p < 0.01), and wheezing episodes per patient (p < 0.001) in the two groups showed a significant reduction in the group receiving resveratrol compared with the placebo group, with less hospital access (p < 0.001) and oral corticosteroid administration (p < 0.01). Our findings seem to suggest that, in non-atopic children with recurrent wheezing secondary to URTIs, nasal resveratrol could be effective to prevent or reduce the occurrence of wheezing, when started from the onset of upper airway symptoms.
Dietary Sodium and Potassium Intakes and Kidney Stone Prevalence: The National Health and Nutrition Examination Survey 2011–2018
Authors: Tang Jie , Sammartino Cara , Chonchol Michel
Published: July 2024

The associations between dietary sodium intake (DSI), dietary potassium intake (DPI), and kidney stone disease (KSD) are not clear. We examined The National Health and Nutrition Examination Survey 2011–2018 to determine the independent associations between daily DSI, DPI, DSI/DPI, and KSD prevalence. In total, 19,405 participants were included for analysis, of which 1,895 had KSD. Higher DSI was not associated with increased odds of KSD in regression analysis when DSI was modeled as a continuous variable (OR = 0.99, 95% CI: 0.99–1.00, p = 0.2), or when comparing highest quartile of DSI to lowest quartile (OR = 0.84, 95% CI: 0.68–1.04, p = 0.1). Unlike DSI, higher DPI was strongly associated with reduced odds of KSD in regression analysis when DPI was modeled as a continuous variable (OR = 0.99, 95% CI: 0.99–0.99, p = 0.02), or when comparing highest quartile of DPI to lowest quartile (OR = 0.75, 95% CI: 0.60–0.94, p = 0.01). Lastly, higher DSI/DPI was also strongly associated with increased odds of KSD in regression analysis when DSI/DPI was modeled as a continuous variable (OR = 1.1, 95% CI: 1.01–1.20, p = 0.03), or when comparing highest quartile of DPI to lowest quartile (OR = 1.30, 95% CI: 1.10–1.70, p = 0.008). All the observed relationships were independent of total calorie intake. In conclusion, both lower DPI and higher DSI/DPI are associated with an increased risk of KSD. Future prospective studies are needed to clarify these causal relationships.
Community-Based Participatory Obesity Prevention Interventions in Rural Communities: A Scoping Review
Authors: Dhanjani Saagar , Allen Haley , Varman Beatriz , Callender Chishinga , Dave Jayna M. , Thompson Debbe
Published: July 2024

Child obesity is a worldwide public health concern. In America, children from rural areas have greater odds of obesity in comparison to those from urban areas. Community-engaged research is important for all communities, particularly under-represented communities. This paper reports the results of a scoping review investigating community-engaged research in obesity prevention programs tested with school-aged children in rural America. A literature search of Medline Ovid was conducted to identify interventions reporting the results of obesity prevention interventions that promoted a healthy diet or physical activity (PA) behaviors to school-age children in rural communities of the United States (US). After title and abstract review, potentially relevant citations were further examined by assessing the full text. Each stage of review was conducted by two independent reviewers. Twelve studies met the inclusionary criteria and are included in this review. Most of the studies focused on elementary school participants (n = 7) and improving both diet and PA (n = 9). Out of the twelve studies, only five included the target audience in intervention development or implementation. The most popular type of community engagement was community participation (n = 4). This review revealed that community-engaged research is under-utilized in obesity prevention interventions tested with school-aged children in rural US communities.
Impact of Combined Prebiotic Galacto-Oligosaccharides and Bifidobacterium breve-Derived Postbiotic on Gut Microbiota and HbA1c in Prediabetic Adults: A Double-Blind, Randomized, Placebo-Controlled Study
Authors: Beteri Beyda , Barone Monica , Turroni Silvia , Brigidi Patrizia , Tzortzis George , Vulevic Jelena , Sekulic Karol , Motei Diana-Elena , Costabile Adele
Published: July 2024

The complex interactions between intestinal microbiota and metabolic disorders are well-documented, with implications for glucose metabolism, energy expenditure, and intestinal permeability. Prebiotics induce beneficial changes in gut microbiota composition in prediabetes, while postbiotics can enhance gut barrier function, complementing each other to improve glucose metabolism and insulin sensitivity. This study investigated the effects of a 12-week dietary fibre (DF) supplement on gut health, metabolic function, and diet. The supplement contained konjac glucomannan (KGM), galacto-oligosaccharides (GOSs), and exopolysaccharides (EPSs) from Bifidobacterium breve. In a randomised, double-blind, placebo-controlled, parallel-group clinical trial, 53 prediabetic volunteers were randomly assigned to either a daily DF supplement (YMETA) or a placebo (cellulose microcrystalline) for 12 weeks, followed by a 4-week follow-up. Measurements included gut microbiota composition, glycated haemoglobin (HbA1c), fasting plasma glucose (FPG), plasma lipids, anthropometry, body composition, blood pressure, and dietary intake. The intervention group showed a significant increase in alpha diversity and butyrate-producing bacteria, with reductions in HbA1c and FPG levels below prediabetes thresholds. No significant changes were observed in the placebo group. This study suggests that manipulating the human gut microbiome through dietary interventions could be a promising therapeutic approach to managing prediabetes and preventing or delaying diabetes.
The Protective Effect of Flavonoids in the Diet on Autophagy-Related Cardiac Impairment
Authors: L’Abbate Serena , Kusmic Claudia
Published: July 2024

The compounds known as flavonoids, commonly found in fruits, vegetables, legumes, medicinal herbs, chocolate, and coffee and tea beverages, have been extensively researched for their impact on cardiovascular health. Flavonoids, with their demonstrated potential, have shown promising effects in regulating blood vessel function and apoptotic processes, as well as in improving lipid profiles. While their powerful antioxidant properties were initially thought to be the main reason behind these effects, recent studies have uncovered new insights into the positive effects of flavonoids on cardiovascular health, and researchers have now identified several signaling pathways and mechanisms that also play a role. Of particular interest are the studies that have highlighted the role of autophagy in maintaining the physiological functions of cardiomyocytes and protecting them from harm. Recent publications have linked the dysregulation of autophagic processes with the development of cardiomyopathies, heart failure, and other cardiovascular diseases. This review aims to present the latest, novel findings from preclinical research regarding the potential beneficial effects of flavonoids on various heart conditions associated with altered autophagy processes.