bluuebunny/crossref_metadata_2025 huggingface dataset:
NKX6.3 modulation of mitotic dynamics and genomic stability in gastric carcinogenesis
Authors: Yoon Jung Hwan , Kim Jeong-Kyu , Eun Jung Woo , Ashktorab Hassan , Smoot Duane T. , Nam Suk Woo , Park Won Sang
Published: November 2024

Background
Gastric cancer remains a significant global health challenge, characterized by poor prognosis and high mortality rates. Mitotic integrity and genomic stability are crucial in maintaining cellular homeostasis and preventing tumorigenesis. The transcription factor NKX6.3 has emerged as a potential regulator of these processes in gastric epithelial cells, prompting an investigation into its role in gastric cancer development.
Methods
We employed a combination of in vitro and in vivo techniques to elucidate the impact of NKX6.3 depletion on mitotic dynamics and genomic stability in gastric epithelial cells. Quantitative real-time PCR and Western blot analyses were conducted to assess the expression of mitosis-related genes and proteins. Flow cytometry was utilized to evaluate cell cycle distribution, while immunofluorescence microscopy enabled the visualization of mitotic abnormalities. Statistical analyses, including Student's t-test and ANOVA, were performed to determine the significance of our findings.
Results
Our results demonstrate that NKX6.3 depletion leads to significant mitotic defects, characterized by increased chromosome misalignment and lagging chromosomes during anaphase. These abnormalities corresponded with elevated levels of genomic instability markers, indicating compromised genomic integrity. Furthermore, the loss of NKX6.3 resulted in altered expression of key regulatory proteins involved in mitosis and DNA repair pathways, suggesting a mechanistic link between NKX6.3 and the maintenance of genomic stability in gastric epithelial cells. Depletion of NKX6.3 resulted in accelerated cell cycle progression and the formation of abnormal mitotic figures, leading to genomic instability characterized by increased DNA content and structural abnormalities. In both in vitro and xenograft models, the depletion of NKX6.3 significantly upregulated AurkA and TPX2, which correlated with gains in DNA copy number. An inverse relationship was observed between NKX6.3 expression and the levels of AurkA and TPX2 in human gastric cancer tissues.
Conclusions
This study highlights the essential role of NKX6.3 in regulating mitotic integrity and genomic stability in gastric carcinogenesis. The findings suggest that targeting NKX6.3 may offer a novel therapeutic strategy for improving treatment outcomes in gastric cancer by restoring mitotic fidelity and genomic stability.
Trial registration
This study was not registered.
Development and Validation of a Framework for Registration of Whole-Mount Radical Prostatectomy Histopathology with Three-dimensional Transrectal Ultrasound
Authors: Jager Auke , Zwart Marije J. , Postema Arnoud W. , Kroonenberg Daniel L. , Zwart Wim , Beerlage Harrie P. , Oddens J. R. , Mischi Massimo
Published: November 2024

Purpose
Artificial intelligence (AI) has the potential to improve diagnostic imaging on multiple levels. To develop and validate these AI-assisted modalities a reliable dataset is of utmost importance. The registration of imaging to pathology is an essential step in creating such a dataset. This study presents a comprehensive framework for the registration of 3D transrectal ultrasound (TRUS) to radical prostatectomy specimen (RPS) pathology.
Methods
The study enrolled patients who underwent 3D TRUS and were scheduled for radical prostatectomy. A four-step process for registering RPS to TRUS was used: image segmentation, 3D reconstruction of RPS pathology, registration and ground truth calculation. Accuracy was assessed using a target-registration error (TRE) based on landmarks visible on both TRUS and pathology.
Results
20 Sets of 3D TRUS and RPS pathology were included for analyses. The mean TRE was 3.5 mm, ranging from 0.4 to 5.4 mm.
Conclusion
The framework proposed in this study accomplishes precise registration between prostate pathology and imaging. The methodologies employed hold the potential for broader application across diverse imaging modalities and other target organs.
BayesianFitForecast: A User-Friendly R Toolbox for Parameter Estimation and Forecasting with Ordinary Differential Equations
Authors: Karami Hamed , Bleichrodt Amanda , Luo Ruiyan , Chowell Gerardo
Published: November 2024

Background: Mathematical models based on ordinary differential equations (ODEs) are essential tools across various scientific disciplines, including biology, ecology, and healthcare informatics, where they are used to simulate complex dynamic systems and inform decision-making. In this paper, we introduce BayesianFitForecast, an R toolbox specifically developed to streamline Bayesian parameter estimation and forecasting in ODE models, making it particularly relevant to health informatics and public health decision-making (https://github.com/gchowell/BayesianFitForecast/).
Results: This toolbox enables automatic generation of Stan files, allowing users to configure models, define priors, and analyze results with minimal programming expertise. To demonstrate the versatility and robustness of BayesianFitForecast, we apply it to the analysis of the 1918 influenza pandemic in San Francisco, comparing Poisson and negative binomial error structures within the SEIR model. We also test it in case of fitting to multiple time series of state variables using simulated data. BayesianFitForecast provides robust tools for evaluating model performance, including convergence diagnostics, posterior distributions, credible intervals, and performance metrics.
Conclusion: By improving the accessibility of advanced Bayesian methods, this toolbox significantly broadens the application of Bayesian inference methods to dynamical systems critical for healthcare and epidemiological forecasting. Additionally, we include a tutorial video demonstrating the toolbox’s functionality (https://youtu.be/jnxMjz3V3n8).
Unlocking Safer Outcomes: A Meta-Analysis Comparing Dorgan's Lateral Cross-Pinning and Traditional Mediolateral Pinning in Pediatric Supracondylar Fractures
Authors: Khan Muhammad Waqas , Irfan Shayan Ali , Waseem Radeyah , Hussain Muhammad Sheheryar , Sultan Syed Muhammad Moaaz Bin , Aftab Aleena , Naqvi Anum , Fatima Alina , Raza Mahnoor , Solangi Shahzeb , Mohammad Jemal Girma
Published: November 2024

Objective: This study aims to compare the safety and efficacy of two pinning techniques, namely conventional mediolateral pinning and Dorgan's lateral cross-pinning, for treating Gartland type II, III, and IV supracondylar fractures in children.
Methods: A systematic review and meta-analysis were conducted following PRISMA guidelines. PubMed/Medline, Cochrane Trial Register, and Google Scholar were searched for studies comparing the two pinning techniques. Inclusion criteria involved Gartland Type II, III, and IV fractures in children, randomized controlled or observational studies, and outcomes related to functional results and ulnar nerve impingement. Quality assessment was performed using the Newcastle-Ottawa Scale.
Results: Four studies with a total of 282 participants were included. The Dorgan technique demonstrated comparable outcomes to mediolateral pinning in terms of carrying angle and range of motion. However, Dorgan's technique significantly reduced the risk of ulnar nerve impingement compared to conventional pinning (Risk Difference = -0.09, 95% CI = -0.13, -0.04, p = 0.0002).
Conclusion: In the management of Gartland type II, III, and IV supracondylar fractures in children, Dorgan's lateral cross-pinning technique presents a safer alternative to conventional mediolateral pinning by significantly decreasing the risk of ulnar nerve injury. While both techniques offer comparable functional outcomes, clinicians should weigh the benefits of reduced nerve complications against potential radial nerve risks and longer treatment times associated with Dorgan's technique. Individualized decision-making considering patient factors is crucial in selecting the appropriate pinning method for optimal fracture management.
Bias Light Driving Enables Bandwidth Tunable Photodetectors and Its Applications
Authors: Zhao Suling , Zhang Yu , Li Yaoyao , Li Xiaowei , Huo Xiaomin , Liu Heng , Liang Zhiqin , Qiao Bo , Song Dandan , Wageh Swelm , Yuan Guangcai
Published: November 2024

Spectrum selective photodetection is urgently demanded and expected to play a disruptive role in optical communications and surveillance. Facilitated detection spectrum tuning of photodetector can be achieved by architecture engineering or bias voltage modulation, which are attributed to carrier regulation from the internal mechanisms. However, the unsatisfactory inefficient carriers manipulating method for the conventional implementation strategies has become the main factor limiting the performance, causing inevitable parameter trade-offs regarding responsivity and response speed. In this work, we demonstrate a series of perovskite/organic integrated photodetectors with tunable bandwidth by manipulating photogenerated carriers with a bias light. The additional bias light was verified to manipulate the transportation and recombination processes of photogenerated carriers of the probe signal. Consequently, a narrowband detector with a response in the near-infrared range and a spectral rejection ratio reaching 1547 was developed. Moreover, a self-driven broadband detector with ultra-high external quantum efficiency that approaches the theoretical limit, reaching 96.68% at 820nm, with a response range covering 300-1000nm, has been developed by boosting bias light. This work provides one of the first investigations into photoelectric conversion under dual-component light conditions. The innovative bias light driving approach and impressive performance offer a fresh perspective on carrier regulation and optoelectronic device investigation.
Impact of Mild Traumatic Brain Injury on Health Behaviors: A Korean Nationwide Population-Based Study
Authors: Lee Yookyung , Choi Yoonjeong , Jeon Jooeun , Leigh Ja-Ho , Kim Don-Kyu , Oh Byung-Mo
Published: November 2024

Health-related behavioral changes can occur following traumatic brain injury. We focused on understanding the impact of mild traumatic brain injury on health-related behaviors and identifying factors associated with such changes. We utilized health check-up records from the Korean National Health Insurance Service database spanning January 1, 2009, to December 31, 2017. The sample included 49,212 patients diagnosed with mild traumatic brain injury and 1:1 matched controls who participated in national health check-ups in 2009–2010, 2011–2012, and 2016–2017. Multivariable logistic regression analysis was utilized to examine the association between mild TBI and short- and long-term health-related behavioral changes. Mild traumatic brain injury was significantly associated with an increased risk of insufficient physical activity at the short- [odds ratio (OR), 1.04; 95% confidence interval (CI), 1.01 − 1.07] and long-term (OR, 1.06; 95% CI, 1.03 − 1.09) follow-ups. Age ≥ 65 years and female sex were significant effect modifiers for in sufficient physical activity (OR, 1.11; 95% CI, 1.02 − 1.21; p < 0.001) and smoking (OR, 1.31; 95% CI, 1.14 − 1.51; p < 0.001), respectively. Mild traumatic brain injury can lead to detrimental health-related behavioral changes, varying by age and sex. Thus, age- and sex-specific interventions are needed to address these changes.
One-Year Epidemiological Patterns of Respiratory Pathogens Across Age, Gender, and Seasons in Chengdu During the Post-COVID Era
Authors: Li Xiang , Ma Jian , Li Yi , Hu Zhongliang
Published: November 2024

Respiratory tract infections caused by various pathogens remain a significant public health concern due to their high prevalence and potential for severe complications. This study systematically analyzed the epidemiological characteristics of six common respiratory pathogens—Chlamydia pneumoniae (CP), Mycoplasma pneumoniae (MP), Adenovirus (AdV), Influenza A virus (FluA), Influenza B virus (FluB), and Respiratory Syncytial Virus (RSV)—in patients from Sichuan Jinxin Xinan Women and Children’s Hospital between April 2023 and March 2024. Over 100,000 throat swab samples were collected and processed using the AUTOMOLEC 3000 analyzer, with PCR-fluorescent probe method employed for pathogen detection. The results showed that MP had the highest overall positive rate (21.83%), followed by FluA (17.50%) and FluB (14.84%). MP showed the highest mean monthly (average) positive rate (16.84% ± 8.41). Significant differences were found between MP and AdV, CP and RSV in average positive rate (p < 0.05). Co-infection analysis revealed frequent associations between MP and AdV, MP and CP, and FluB with MP. Seasonal analysis indicated distinct peaks: FluA and FluB in winter, RSV in spring, and MP in summer, autumn and winter. Age-stratified analysis showed higher positivity rates of RSV in children aged 0–6 years, MP and CP in the 7–17 years group. No significant sex-based differences were observed for the pathogens. These findings provide crucial insights into the prevalence and seasonal distribution of respiratory pathogens in Chengdu, offering valuable data to inform public health strategies in the post-COVID era.
Implementation cost analysis of collaborative care for perinatal mental health in community health centers
Authors: Grover Tess , Bennett Ian M , Campbell Mark , Vredevoogd Melinda , Saldana Lisa
Published: November 2024

Background
Although costs are of key importance to clinic leadership when considering adoption of new programs, few studies examine real-world resource needs associated with implementing complex interventions for chronic conditions in primary care. This analysis sought to identify the costs necessary to implement the evidence-based collaborative care model (CoCM), an integrated behavioral health program for common mental disorders in primary care.
Methods
Ten federally qualified health centers (FQHCs) adopted CoCM as part of a larger national randomized trial evaluating implementation strategies for CoCM when adapted for perinatal mental health. The Cost of Implementing New Strategies (COINS) tool was used to assess implementation costs associated with activities completed by sites as they progressed through the implementation process. National wage norms were used to calculate cost estimates for staff time.
Results
On average, clinics spent $40,778 (SD=$30,611) on implementation, with clinics ranging widely from $4,502 to $103,156. Three out of 10 participating clinics achieved competency in the intervention during the 2-year implementation period. Costs among competent clinics ranged from $20,944 to $65,415 (mean=$41,788). Clinics that did not achieve competency were more varied, with both the lowest and highest resource use. Significant staff effort was required to complete all implementation stages; clinical staff and program champions showed greatest effort.
Conclusions
Site implementation costs for this complex behavioral health intervention were substantial and varied dramatically, particularly among sites who did not achieve competence. Additional work is needed to identify optimal site resource investment related to implementation success for CoCM.
Trial registration:
ClinicalTrials.gov.NCT02976025. Registered on November 23, 2016.
BRD2 Phase Separation Promotes Ferritinophagy in Depression
Authors: Li Zhen , Wang Xiaoling , Li Qinglian , Liu Yangping , Si Wenwen , Chen Dongfeng , Wang Qizhang , Zhu Meiling
Published: November 2024

Background Depression is a psychiatric disorder which affects many aspects of social life of patients; however, the molecular biological mechanisms underlying its development are not fully understood. Atypical phase separation is a mechanism contributing to the occurrence of neurological disorders. The interaction between autophagy and iron metabolism have been implicated in the pathogenesis of neurological diseases.Methods To explore the pathogenesis of depression, we analyzed super-enhancers (SEs) in the prefrontal cortex of depression model rats using chromatin immunoprecipitation sequencing (ChIP-seq). The role of the upstream protein Bromodomain Containing 2 (BRD2) in super-enhancers (SEs) was investigated by synthesizing liquid-liquid phase separation proteins in vitro which were examined through Fluorescence recovery after photobleaching (FRAP) analysis. Moreover, the markers of ferritinophagy in cortisol-stimulated primary cortical neuron cells and PC12 cell models were explored to the occurrence of ferritinophagy in depression models.Results Rats exposed to chronic mild stress (CMS) exhibited decreased activation of autophagy-related 7 (ATG7) mediated by SEs. In rats subjected to CMS and in cellular models of depression, ferritinophagy was detected. Arid5a stimulated ATG7 activation via SEs and was influenced by the BET family and liquid-liquid phase separation. Ferritinophagy dysfunction in cellular models of depression was dependent on ATG7. BRD2 exhibited aberrant phase separation in CMS rats. The BET inhibitor JQ1 alleviated depressive behavior in rats subjected to CMS.Conclusions The ATG7 genes are activated by SEs in cellular and animal models of depression. The increased transcriptional activity of ATG7 can increase the risk of ferritinophagy. Moreover, the phase separation of BRD2 promotes ATG7 expression via SEs.
Photoenhanced Field-Emission Nano-Air-Channel Devices for Terahertz Generation
Authors: Chen Feiliang , Li Mo , Li Xiaoxu , Sun Lixin , Li Xiangyang , Jiang Hao , Yang Fan , Liu Yang , Peng Gang-Ding , Zhang Jian
Published: November 2024

Nano-air-channel devices (NACDs), characterized by scattering-free ballistic electron transport in a quasi-vacuum channel, provide a new opportunity for vacuum electronics and nanoelectronics. However, enabling the NACDs for high-frequency operation is still a great challenge due to the low field-emission current and high impedance. Herein, for the first time, field-emission NACDs are demonstrated as photomixers capable of generating coherent terahertz (THz) signals from 120 GHz to 260 GHz. This achievement marks the first successful experimental demonstration of NACDs operating beyond 100 GHz. Vertical configuration NACDs with InP/InGaAs heterojunction photocathodes and 50 nm in-plane nano-air channels are designed and fabricated using a wafer-scale manufacturing process. The field-emission currents show an impressive 375-fold increase with a low-power 1550 nm CW laser irradiation. A remarkably high internal quantum efficiency of up to 1600% is achieved by exploiting the carrier multiplication effect within the heterojunction photocathodes. Furthermore, the NACDs exhibit reproducible photo-switching behavior and highly stable field-emission current even at temperatures as high as 250 ℃. These findings position NACDs as new promising candidates for photonic THz generators, opening up an exciting application prospect for NACDs in THz high-speed wireless communications, 6G networks and high-resolution radar.
Exploring Trauma Recovery in Nurses: A Text Mining and Thematic Analysis Based on Swanson’s Theory of Caring
Authors: Park Jinyoung , Kim Go-Un , Kim Sunah
Published: November 2024

Background
Nurses are frequently exposed to workplace trauma, placing them at a heightened risk of post-traumatic stress disorder. However, targeted interventions to promote psychological recovery among nurses are limited. This study explored the trauma recovery experiences of nurses who participated in an Internet-based Trauma Recovery Nursing Intervention (IBTRNI), based on Swanson’s Theory of Caring. The objective was to identify the emotional and psychological changes experienced by participants through a combination of text mining and thematic analysis.
Methods
Secondary analysis was conducted on free-text responses from 102 nurses who completed IBTRNI. Text mining identified high-frequency keywords, while thematic analysis provided deeper emotional and psychological insights. The analysis was structured around Swanson’s three phases: “Knowing,” “Doing For,” and “Enabling.”
Results
In the “Knowing phase,” the participants demonstrated increased self-awareness, recognizing their emotional responses and the effects of negative thoughts on daily life. The “Doing For” phase revealed enhanced emotional regulation, where participants learned to manage and transform negative emotions into positive ones. Finally, the “Enabling” phase highlighted improvements in interpersonal relationships, and the adoption of effective coping mechanisms such as communication and meditation to manage stress.
Conclusions
Swanson’s Theory of Caring provides a robust framework for supporting nurses' trauma recovery. The combination of text mining and thematic analysis offers a comprehensive understanding of the emotional and psychological transformations experienced during the intervention. The findings underscore the potential for theory-based digital interventions to support trauma recovery among healthcare professionals. Future research should expand on these methodologies to enhance their broader applicability.
Trial registration
This study involved secondary data analysis. The primary study was registered at ClinicalTrials.govUS National Library of Medicine (clinical trial registration number NCT04989582) on 20220131 and is available online.
Juvenile Cellulitis in a Labrador retriever Puppy: A Case Report
Authors: Shaji Namitha , R Sindhu K , David Vinu
Published: November 2024

Juvenile cellulitis, commonly known as puppy strangles, is a rare dermatological disorder affecting puppies, characterised by acute swelling, pustular dermatitis and lymphadenopathy. This autoimmune disease is of unknown etiology but is thought to have a genetic predisposition. An eight-week-old Labrador retriever puppy was presented with the history of nodular skin lesions and facial oedema that was not resolving with antibiotic therapy. Clinical examination revealed lymphadenopathy and secondary otitis. From skin scrapings, lymph node cytology, bacterial culture and response to therapy, the condition was diagnosed as puppy strangles. The pup made a full recovery upon high-dose glucocorticoid therapy.
Subdural Hematoma as a Complication of Endoscopic Third Ventriculostomy in a Pediatric Patient: A Case Report & Literature Review
Authors: Fernandes Rafael Tiza , Sobrinho Rui , Azevedo Miguel , Matos Mário , Sagarribay Amets , Forte Dalila
Published: November 2024

Background: Subdural hematoma (SDH) typically occurs due to traumatic brain injury but can arise as a rare complication of procedures like endoscopic third ventriculostomy (ETV).
Case Presentation: We report an unusual case in a 9-year-old male with previous resection of a fourth-ventricle ependymoma at 2 years of age. Seven years post-surgery, he presented with worsening hydrocephalus and underwent ETV. One month later, he developed severe headaches and motor difficulties. Imaging revealed a significant right SDH, necessitating urgent drainage. Postoperative recovery was uneventful, and follow-up imaging showed resolution of the hematoma.
Literature Review & Discussion: ETV is generally preferred for obstructive hydrocephalus due to lower complication rates compared to shunt procedures. However, cases of SDH post-ETV remain reported, albeit rarely. Potential mechanisms include altered cerebrospinal fluid dynamics and intraoperative vessel injury. This case aligns with literature findings and reinforces the importance of postoperative monitoring and prompt intervention in symptomatic cases to prevent complications.
Conclusion: Clinicians should consider SDH in pediatric patients with new symptoms post-ETV. Further research should focus on understanding the risk factors and mechanisms for SDH development.
EyeDiff: text-to-image diffusion model improves rare eye disease diagnosis
Authors: Chen Ruoyu , Zhang Weiyi , Liu Bowen , Chen Xiaolan , Xu Pusheng , Liu Shunming , He Mingguang , Shi Danli
Published: November 2024

The rising prevalence of vision-threatening retinal diseases poses a significant burden on the global healthcare systems. Deep learning (DL) offers a promising solution for automatic disease screening but demands substantial data. Collecting and labeling large volumes of ophthalmic images across various modalities encounters several real-world challenges, especially for rare diseases. Here, we introduce EyeDiff, a text-to-image model designed to generate multimodal ophthalmic images from natural language prompts and evaluate its applicability in diagnosing common and rare diseases. EyeDiff is trained on eight large-scale datasets using the advanced latent diffusion model, covering 14 ophthalmic image modalities and over 80 ocular diseases, and is adapted to ten multi-country external datasets. The generated images accurately capture essential lesional characteristics, achieving high alignment with text prompts as evaluated by objective metrics and human experts. Furthermore, integrating generated images significantly enhances the accuracy of detecting minority classes and rare eye diseases, surpassing traditional oversampling methods in addressing data imbalance. EyeDiff effectively tackles the issue of data imbalance and insufficiency typically encountered in rare diseases and addresses the challenges of collecting large-scale annotated images, offering a transformative solution to enhance the development of expert-level diseases diagnosis models in ophthalmic field.
Occupational Histories in Neuropathologically Confirmed Multiple System Atrophy
Authors: Cheshire William P. , Tipton Philip W. , Koga Shunsuke , Sekiya Hiroaki , Uitti Ryan J. , Ross Owen A. , Heckman Michael G. , Sledge Hanna J. , Dickson Dennis W.
Published: November 2024

Purpose
This study examined occupational histories in neuropathologically confirmed cases of multiple system atrophy to identify environmental associations of potential relevance to disease causation.
Methods
270 neuropathologically confirmed cases of multiple system atrophy obtained from the Mayo Clinic brain bank for neurodegenerative disorders in Jacksonville, Florida, were included in this case-control study. Demographic and disease information was collected from medical records. Information regarding occupational history was collected retrospectively from medical records and published obituaries. Proportions of employment by occupational sector were compared to United States census data.
Results
The most common prior occupations in patients were healthcare (15.2%), education (15.2%), administration (14.8%), clerical (10.7%), petroleum industry (8.9%), business ownership (8.5%), and metal industry (7.8%). When comparing patients to United States census data, significant differences were identified for electrical (5.6% vs. 0.4%, P < 0.001), education (15.2% vs. 2.3%, P < 0.001), real estate (4.4% vs. 0.7%, P < 0.001), metal industry (7.8% vs. 3.0%, P < 0.001), information technology (4.1% vs. 1.8%, P = 0.011), clerical (10.7% vs. 5.5%, P = 0.001), and petroleum industry (8.9% vs. 5.6%, P = 0.024).
Conclusions
Electrical engineers and electricians, educators, real estate agents, workers in the metal industry, information technologists, clerical staff, and workers in the petroleum industry were significantly overrepresented in our series of multiple system atrophy patients as compared to population data. We hypothesize that these occupational associations may signify environmental exposures increasing disease risk in genetically susceptible individuals. We cannot exclude a potential selection bias in patients willing to donate their brains to an academic center in order to contribute to scientific knowledge.
Impact of leaflet resection technique on mitral valve mobility after mitral valve repair assessed by echocardiography
Authors: Yuge Norihisa , Manabe Susumu , Hirayama Daiki , Yamada Ryuki , Hori Mariko , Sugimura Koichiro , Ushijima Teruaki , Shimokawa Hiroaki
Published: November 2024

BACKGROUND: Two surgical techniques, leaflet resection and leaflet preservation, are widely used for mitral valve repair insevere mitral regurgitation due to posterior mitral leaflet prolapse. However, which technique is superior remains unclear. The leaflet resection technique may affect the postoperative mitral valve function differently from the leaflet preservation technique because it causes anatomical alterations in the corrected leaflet. We aimed to evaluate the effect of the leaflet resection technique on mitral valve mobility, compared with that of the leaflet preservation technique.
METHODS: Forty-one patients underwent mitral valve repair for P2 prolapse. Among them, 27 underwent leaflet preservation and 14 underwent leaflet resection. We examined the effects of the leaflet resection technique on the mitral valve mobility.
RESULTS: Postoperatively, the mobility of the corrected leaflet was significantly decreased in the leaflet resection group (leaflet preservation: 35.1±13.8% vs. leaflet resection: 22.7±13.7%, p=0.009). Particularly, the maximum closed angle was significantly decreased (leaflet preservation: 29.1±11.4% vs. leaflet resection: 40.7±12.0%, p=0.004). Therefore, the closing motion of the resected leaflet was more restricted than its opening motion. Mitral valve function, including mitral valve area, and peak and mean transmitral pressure gradients were comparable in both groups.
CONCLUSIONS: Although there were no differences in the mitral valve function, the leaflet preservation technique may be more effective than the leaflet resection technique at preserving the corrected leaflet mobility.
Quality of registration and adherence to guidelines for blood management in CABG surgeries: a case study
Authors: De Luca Giulia M.R. , Vlot Eline , Dijk Thomas van
Published: November 2024

In many hospitals, patients undergoing cardiac surgery receive a higher amount of blood products transfusions compared to other disciplines. Blood transfusion relates with increased morbidity and mortality across different patient groups. Furthermore, blood products are scarce and the new blood safety technologies come at high costs. More specifically patients undergoing cardiac surgery that received perioperative blood transfusions show higher risk of mortality and in general worse morbidity outcomes than non-transfused patients do, with a dose-dependent association between transfusion and development of postoperative infection. Patient Blood Management (PBM) practices focus on the application of evidence based medical and surgical concepts in order to preserve the patient’s own blood. Unfortunately, studies suggest that only a small fraction of published guidelines are implemented and followed into daily clinical practice. Many barriers limit the translation of guidelines for PBM into clinical practice worldwide, such as clear guidance, concerns about risks, and lack of knowledge, interdisciplinary commitment or resources. The widespread adoption of electronic health record (EHR) offers the opportunity to improve clinical outcomes through use of decision support system to guide the healthcare providers through best practices and guidelines. Decision support systems can be active, data-based, patient-specific and act timely, and can be more useful that adding new clinical practice guidelines. This case study quantifies the quality of the data registration and provides the results for adherence to perioperative PBM guidelines for coronary artery bypass grafting (CABG) procedures during a three-year period (2018 to 2020), in the St. Antonius hospital, a single heart center that performs over 10% of the total number of heart operations in the Netherlands. With this case study we identify some of the possible improvement factors for PBM in our center. We also quantify the impact of the quality of the registration in the EHR on the analysis results and on possible implementation of decision support systems.
Hypermethylated CACNB2 Predicts Poor Prognosis And Promotes Proliferation, Invasion and Apoptotic Resistance in Pancreatic Ductal Adenocarcinoma
Authors: Jia Xiya , Li Meng , Deng Jixiang , Liu Qin , Ren Zhonggan , Dai Sheng , Chen Bing
Published: November 2024

Introduction:
Pancreatic ductal adenocarcinoma (PDAC) is one of the most malignant cancers and its 5-year survival rate is less than 10%. DNA-methylation emerges as a pivotal factor in the carcinogenesis, progression and chemoresistance of PDAC. However, numerous methylation-regulated genes and their tumor-promoting mechanisms remain largely unknown.
Methods
The TCGA database was analyzed to identify CACNB2 with high methylation levels, which is significantly associated with unfavorable prognosis and clinical characteristics. Low CACNB2 expression in PDAC and its clinical value were further evaluated through immunohistochemistry and immunofluorescence on our tissue microarray. Functional experiments, including CCK-8, colony formation assay, invasion assay, and flowcytometry analysis for apoptosis and cell cycle, were conducted to reveal the oncological value of CACNB2 in PDAC cell lines. Bisulfate sequencing was used to detect DNA methylation level of CACNB2, while transcriptome sequencing and western blotting were used to uncover underlying molecular mechanisms.
Results
CACNB2 promoter was hypermethylated, which was negatively correlated with its expression in both PDAC samples from TCGA and PDAC cell lines. Downregulated CACNB2 independently predicted an unfavorable prognosis and was associated with inferior clinicopathological features, including poor tumor differentiation, advanced T stage, advanced N stage, and advanced AJCC stage, in PDAC patients from TCGA and our center. The overexpression of CACNB2 exerted inhibitory effects on proliferation and invasion, while promoting apoptosis and tumor-suppressing pathways, including ANKRD1, CDKN1A and phosphorylated p53, in PDAC cell lines. Moreover, PDAC cell lines could recover the CACNB2 expression via decitabine-induced demethylation.
Discussion
These data suggested that CACNB2 hypermethylation contributed to PDAC progression and it might be a potential therapeutic target for treating PDAC.
A Case Report of Rosai-Dorfman Disease in a 9-Year-Old Girl with Left Tibia Involvement: Insights into Etiology, Clinical Presentation, and Treatment Advances
Authors: Ali Khan Akhtar , Bao Xin , Li Mi , safari Elaheh , Huang Hui , Li Hao , Li Xiaojuan
Published: November 2024

This case report describes the medical journey of a 9-year-old girl who was diagnosed with Rosai-Dorfman disease (RDD), a rare disorder characterized by the overproduction of histiocytes within the lymph nodes. The patient was diagnosed with RDD after experiencing left calf pain and discomfort. Initial imaging revealed abnormal signals and localized low-density foci in the left tibia, and subsequent MRI scans indicated a possible tumor. The diagnosis was confirmed through a puncture biopsy, which revealed granulomatous tissue consistent with RDD. The patient's treatment focused on immobilizing the affected limb without surgical intervention. Follow-up imaging showed a reduction in the extent of the initial abnormalities and periosteal reaction. This case emphasizes considering Rosai-Dorfman disease (RDD) in pediatric bone lesion diagnoses and highlights the effectiveness of conservative management. The study provides a comprehensive overview of RDD in the skeletal system, including etiology, clinical manifestations, imaging features, diagnostics, and therapeutic strategies, serving as a valuable reference for clinicians and future research.
Analysis of Filial Ethics of Offspring in the Care Process of Disabled Elderly in the Context of Low Birth and Aging Population: A Cross‑sectional Study
Authors: Ma Xiaobin , Deng Huanhuan , Chen Changxiang , hao xijun
Published: November 2024

Background
With the rapid aging of the population and a significant decline in fertility rates, elderly individuals with disabilities face enormous pressure to meet their old-age needs. The filial beliefs and behaviors of offspring play a crucial role in the physical and mental health of elderly disabled parents. We conducted a survey with the aim of assessing and analyzing the factors influencing the ethical status of filial piety among offspring caregivers of the elderly individuals with disabilities.
Methods
A cross-sectional descriptive study was conducted on 528 offspring caregivers of the disabled elderly hospitalized in three secondary hospitals and three tertiary hospitals in Tangshan City, Hebei Province, China, and the questionnaire prepared and validated in advance by the subject group to investigate the filial piety ethics of the offspring caregivers of the disabled elderly. Descriptive statistics were used to analyze the data; t-test and ANOVA were used to compare differences between groups. Variables with a significance level of 0.05 in the univariate analysis were entered into the multivariate regression analysis to determine the predictors.
Results
The filial elder ethics score of the offspring caregivers was (105.23 ± 0.515), which is above average. The Educational level of the offspring caregivers, self-perceived economic status, age of the disabled elderly, disability status in the disabled elderly, support and care of the couple of the offspring caregivers, whether or not the neighbors provide caregiving assistance, and the cost of medication, medical treatment, and caregiving accounts for the proportion of economic expenditure of the offspring caregivers were the factors affecting the level of filial old age ethic (β = -2.356, -1.760, -2.657, -7.550, 1.167, -3.521, -2.291, P < 0.05).
Conclusions
The ethical status of filial piety of the offspring's caregivers needs to be further improved, and individuals, families, and community、governments need to work together to improve the ethical status of filial ethics of the offspring's caregivers of the disabled elderly.
Trial registration
Not applicable.
A novel high-conjugated probe of 4-(acridone-10-yl)-phenylethyl chloroformate (ABE-Cl) for rapid detection of amino compounds Using HPLC with Fluorescence detection
Authors: Lou Yingying , Xiao Huiquan , Zou Xiaocong , Xu Zenghui , Sun Zhiwei , Li Zan , Ji Zhongyin , You Jinmao
Published: November 2024

The fluorescence detection of amino compounds and the evaluation of their content in environmental samples are vital, not only for assessing food quality but also for studying soil organic matter. Here, we present the synthesis and application of a novel fluorescent probe, 4-(9-acridone)benzylmethyl carbonochloride (APE-Cl), for detecting amino compounds via a chloromate reaction with fluorescence detection. The complete derivatization reaction of APE-Cl with amino compounds can be accomplished in aqueous acetonitrile within 5 minutes at room temperature, using 0.2 M borate buffer (pH = 9.0). APE-amine derivatives exhibited intense fluorescence with an excitation maximum at λex 254 nm and an emission maximum at λem 418 nm. All derivatives demonstrated high stability, strong fluorescence, and elevated ionization potential under atmospheric pressure chemical ionization (APCI-MS) in positive ion detection mode. The method, combined with gradient elution, provides baseline resolution of common amine derivatives on a reversed-phase C18 column. The LC separation for the derivatized amines shows good reproducibility with aqueous acetonitrile as the mobile phase. The relative standard deviations (n = 6) for each amine derivative are < 3.99%. The detection limits (at a signal-to-noise ratio of 3) per injection ranged from 1.68 to 11.2 femtomole. The established pre-column derivatization method for determining amino compounds in practical samples proved to be satisfactory.
Prevalence Of Pulmonary Tuberculosis Among Patients with Diabetes Mellitus in Kagera Region, Tanzania.
Authors: MALUGU FRANK , MUGUSI SABINA , AMOUR MARYAM , MAKUKA GERALD , SARAKIKYA EMMANUEL , KIRAVU ANNA
Published: November 2024

Background: Globally and within the African region there is limited knowledge on the double burden of Tuberculosis (TB) and diabetes mellitus (DM) (TB-DM). The risk of acquiring TB among DM patients is three (3) times higher than in the general population. Patients having DM not only have a high risk of getting TB disease but also have poor treatment outcomes.
Methods: This was a cross-sectional study among patients attending diabetic clinics in the Kagera region, Tanzania. Sampling was done using a multi-stage sampling technique to select study participants from hospitals with DM clinics. Data for this study were gathered through the administration of a structured questionnaire and were analyzed using Statistical Package for Social Science (SPSS). Logistic regression was utilized to investigate any associations and control for confounding variables. A p-value below 0.05 was deemed statistically significant in determining the results.
Results: The prevalence of TB among patients with DM in the Kagera region was 14%. Among the socio-demographic findings sex, level of education, residence, facility level, and alcohol consumption were highly associated with TB. In the multivariate analysis, male sex, lack of formal education, lower facility level, private facility ownership, and being co-infected with HIV were significantly associated with a TB diagnosis among the DM patients (p<0.005).
Conclusion: The findings revealed a high prevalence of TB among DM patients in the region, indicating the substantial burden of TB-DM comorbidity. Multiple risk factors were identified leading to a TB diagnosis among the DM patients. Henceforth, public health officials should educate the public on the substantial burden of TB-DM co-infection, and encourage individuals with diabetes to take measures to prevent TBinfection.
Biocomputational analysis to evaluate stacking dual-targeting antibodies in agricultural tobacco plant
Authors: Hwang Hyunjoo , Kim Minkue , Kang Yangjoo , Kim Kibum , Kim Yerin , Lee Dae Hwan , Lee Jeong Hwan , Hinterdorfer Peter , Ko Kisung
Published: November 2024

This study explored the application of tobacco plant expression system for production of dual target monoclonal (mAb) proteins and computational analysis to evaluate their biochemical characteristics. The murine anti-colorectal cancer large single-chain antibody (CL) and H-13F6 human anti-Ebola virus full-size monoclonal antibody (EF) or anti-Ebola large single-chain antibody (EL) co-expressed in F1 plant through crossbreeding transgenic plants expressing each specific antibody. The binding activities of these antibodies to their respective target antigens were then examined. PCR analysis confirmed the presence of the CL gene and the heavy (HC) and light chain (LC) genes of EF in F1 plants resulting from the crossbreeding. Additionally, both CL and EL genes were identified in F1 plants produced through the crossbreeding of transgenic plants expressing each CL and EL. RT-PCR and immunoblot analyses confirmed the mRNA and protein expression of all transgenes in F1 plants. CL, EF, and EL were successfully purified from F1 plants. Indirect ELISA analysis revealed that CL × EF proteins exhibited binding activity to Ebola virus-specific antigenic protein, whereas CL × EL lost this binding activity. Conversely, both CL × EF and CL × EL proteins exhibited enhanced binding activity to the colorectal cancer-specific antigenic protein GA733. In addition, computational analyses [Molecular Dynamics (MD) and Molecular Mechanics Poisson-Boltzmann Surface Area (MM/PBSA) approach] were performed for EBOV antigen-antibody complexes and possible three combinations of Fc consistent with experimental results, supporting our assumption and interpretation.
Integrated Treatment of textile wastewater and Methyl Orange Azo Dye: Constructed Wetland-based Remediation Enhanced by Bacterial Treatment
Authors: Bhargawa Pawan Kumar , Tiwari Shweta , Kumar Saroj , Kumar Rajesh
Published: November 2024

The textile industry significantly contributes to environmental pollution through the discharge of dye-laden wastewater. Among these dyes, methyl orange, an azo dye, is particularly challenging to remove due to its stability and potential toxicity. This study explores an integrated treatment approach combining constructed wetland-based remediation with bacterial treatment to effectively degrade methyl orange and other pollutants in textile wastewater. Textile wastewater was collected from Bhadohi Nagar Palika, Uttar Pradesh, India, and subjected to physicochemical analysis. The initial wastewater exhibited a pH of 9.6 ± 0.12, biochemical oxygen demand (BOD) of 1008 ± 0.75 mg/l, and chemical oxygen demand (COD) of 2035 ± 0.61 mg/l, all exceeding Central Pollution Control Board (CPCB) standards. An 80-liter constructed wetland system planted with Typha latifolia and Phragmites karka was implemented, followed by bacterial treatment. The integrated system reduced the pH to 7.21 ± 0.38, BOD to 54 ± 0.25 mg/l, and COD to 134 ± 0.74 mg/l. Fourier transform-infrared (FTIR) analysis and gas chromatography-mass spectrometry (GC-MS) confirmed significant reductions in organic pollutants and dye components. Additionally, 16S rRNA gene analysis identified key bacterial strains contributing to biodegradation, including Enterococcus faecium and Bacillus subtilis. Further, the treatment system also achieved a notable shift in methyl orange dye absorbance from 538 nm to 201 nm, indicating substantial decolorization. These findings demonstrate the potential of combined wetland and bacterial treatment for effective remediation of textile wastewater.
Rapid search for the parameterized equilibrium of the state-dependent delay differential equation in robotic milling
Authors: Mao SiHao , Jiang YanRu , Ye SongTao , Zhang XiaoJian , Yan SiJie , Ding Han
Published: November 2024

The large forced vibration significantly impacts the tool-workpiece interaction in robotic milling, resulting in the state-dependent delay stability problem (SDD-SP). To solve SDD-SP, it is necessary to search the equilibrium by minimizing the residual of the state-dependent delay differential equation (SDD-DE) with periodic boundary conditions.Previous research has shown that it is complicated and time-consuming because of the nonanalytic iteration process and reduplicative root-finding for SDD.This paper introduces a rapid algorithm for searching for equilibrium using time-domain harmonic-balance-like methods (TDHBM). To solve the time-consuming challenge, the parameterized form of the equilibrium is initially obtained based on Fourier expansion, which spontaneously meets the periodic boundary condition.Then, by substituting the parameterized form into the SDD-DE, the semi-analytic Jacobian matrix for optimization is derived to improve iteration efficiency. Meanwhile, the iteration count for root-finding is significantly reduced by substituting numerical integration of the first-order differential SDD for root-finding.As a result of the simulation, the TDHBM reduces the computation time of searching for the equilibrium by 80% compared with the shooting method (SM), and the equilibrium maintains higher accuracy with the same collocation number.