bluuebunny/crossref_metadata_2025 huggingface dataset:
Immunohistochemical research of reaction of motoneurons of lumbar spinal cord of the mice that were in 30-day flight on the BION-M1 biosatellite on a week readaptation to conditions of Earth gravitation
Authors: Tyapkina O. V , Rezvyakov P. N , Nurullin L. F , Petrov K. A , Nikolskiy E. E , Islamov R. R
Published: October 2024

Earlier, by an immunohistochemical method we define that after 30-day space flight in motoneurons of mice lumbar spinal cord immunoexpression of the proteins responsible for synaptic transfer of a nervous impulse and proteins of heat shock proteins decrease. In this research for an assessment of animals recovery process dynamics after space flight we studied an immunoexpression of the proteins participating in synaptic transfer of a nervous impulse (synaptophisyne, and PSD95), neurotrophic factors (a vascular endothelial factor of growth - VEGF and its receptor - Flt-1) and heat shock proteins (Hsp25 and Hsp70) in motoneurons of lumbar spinal cord of a mice after 30-day space flight on the BION-M1 biosatellite and the subsequent week readaptation to conditions of Earth gravitation. In this research by immunohistochemical method determine that after a week of animals staying in the Earth gravitation conditions the immunoexpression of synapt-ophisyne continued to decrease while the expression of PSD95, Hsp25, Hsp70 and VEGF increased in relation to the animals removed from experiment right after 30-day flight. The obtained data confirm functional plasticity of spinal cord motoneurons in the conditions of gravitation force changing. The fact, which is especially interesting, that «switches on» of neurons protective mechanisms (strengthening of heat shock proteins and neurotrophic factor expression) happens not in response to hypogravi-tation influence, but only a week after return of animals to conditions of Earth gravitation.
Biocompatibility and osteoplastic properties of mineral polymer composite materials based on sodium alginate, gelatin, and calcium phosphates intended for 3D-printing of the constructions for bone replacement
Authors: Karalkin P. A , Sergeeva N. S , Komlev V. S , Sviridova I. K , Kirsanova V. A , Akhmedova S. A , Shanskiy Ya. D , Kuvshinova E. A , Filyushin M. M , Fedotov Yu. A , Teterina A. Yu , Zobkov Yu. V , Barinov S. M , Kaprin A. D
Published: October 2024

Bone extracellular matrix comprises a unique composite compound including the mineral and organic components. Therefore, use of biomimetic approach to the formation of tissue-engineered constructions for bone defects replacement based on composite materials containing biopolymers and calcium phosphates, as expected, can significantly improve their cyto-, biocompatibility and osteoplastic properties. The aim of the work was to study the structural features, biocompatibility and osteoplastic properties of 3D-constructions based on sodium alginate, gelatin, and two types of calcium phosphates (tricalcium phosphate and octacalcium phosphate) obtained by three-dimensional printing. The method of 3D-constructions fabrication comprised inkjet 3D-printing with hydrogel, consisted of alginate and gelatin with the addition of calcium phosphate granules, followed by freezing, freeze-drying and sterilization by y-irradiation. The structure of 3D-constructions, porosity and strength characteristics were evaluated. After the subcutaneous implantation in mice we investigated the biocompatibility of 3D-constructions during the period of up to 12 weeks. Also the osteoplastic properties of the constructions were estimated in vivo in a rat model of tibial defects. 3D printed constructions had irregular lamellar structure of sodium alginate with inclusions of spherical calcium phosphates granules. Addition of gelatin to the composite increased the porosity of constructs and significantly increased the compressive strength meanwhile practically had no effect on the ultimate strain value. In results of subcutaneous in vivo tests 3D printed constructions demonstrated perfect prolonged biocompatibility. The highest rate of biodegradation was noticed for implants containing octacalcium phosphate. All of the studied 3D-scaffolds had osteoconductive potential, more pronounced according to the number of examined histological parameters in those, made from sodium alginate, gelatin and octacalcium phosphate. The data showed the feasibility and prospect of using three-component mineral polymer composite materials based on alginate, gelatin and octacalcium phosphate as an “ink” for 3D printing of bone grafting constructions intended for implantation in bone defects.
OPTIMIZATsIYa TEKhNOLOGII KUL'TIVIROVANIYa DERMAL'NYKh FIBROBLASTOV DLYa TERAPEVTIChESKIKh TsELEY S POMOShch'Yu ROBOTIZIROVANNOY STANTsII
Authors: Fadeyev F. A , Ulitko M. V , Lugovets D. V , Leontyev S. L , Sazonov S. V
Published: October 2024

The potential of the automated cell culture system CompacT SelecT (TAP Biosystems, UK) allows using it for the large scale production of dermal fibroblasts for cell therapy. Automated maintenance and expansion of fibroblasts requires evaluation of the proper seeding density and defining the time for the achievement of the optimal confluency of cell monolayer for further passaging. Objective - to determine the optimal parameters of dermal fibroblasts passaging for development of standardized protocol for automated cell cultivation using CompacT SelecT system. Human dermal fibroblasts were isolated from donor skin samples by enzyme dissociation. Cells proliferation rate was evaluated by the calculation of proliferation index. The dependence of proliferation rate from cell monolayer density was determined using the value of average number of divisions of single cell per day. The dependence of fibroblasts proliferation rate from seeding density (from 50 to 1.2х104cells/cm2) in various periods of cultivation (3rd, 5th, 7th and 9th day) was investigated. The inverse correlation between proliferation rate and seeding density, together with dramatic decrease of proliferation rate at fibroblasts monolayer confluency of (3.5-4.5)х104 cells/cm2, was demonstrated. The optimal seeding density was evaluated as 3х103 cells/ cm2, the most suitable density of cell monolayer for further passaging was evaluated as 3.5х104 cells/cm2. In case of high seeding density (1.2х104 cells/cm2), on day 7, when the cell monolayer density reached the critical value, the cell extinction occured, followed by renewal of cell growth. This phenomenon may be explained by the selection of cells capable of replication under conditions of high density of monolayer.
Determining the number of Ilb / Ilia glycoprotein receptors and expression of P-selectin on the surface of platelets using flow cytometry in patients with acute myeloid leukemia
Authors: Semelev V. N , Tyrenko V. V , Nikitin V. Yu , Sukhina I. A , Yurkin A. K , Tarakanova L. A , Demyanenko N. Y
Published: October 2024

A study of the functional activity of platelets by flow cytometry is conducted in 11 patients with a diagnosis of AML are in clinical remission and 1 1 almost healthy volunteers. The functional activity of platelets was evaluated according to the dynamics of the number of glycoprotein receptors ( GP) IIb/IIIa on the platelet membrane and the percentage of platelets expressing P-selectin (CD62P) before and after induction 10 /тт ADP. The number of GP IIb/IIIa receptors on the platelet surface was evaluated by the mean fluorescence intensity. The average age of the subjects in the group of AML patients was 44,4±5,2 years in the control group, 38,5±6,8 years (p>0,05). In the group of AML patients platelet counts was 104,6±3,1 x109/L in the control group 210,5±20,8x109/L (p0,05) in the number of receptor GP IIb/IIIa before and after ADP stimulation in both groups have been identified. At the same time, there was no statistically significant difference (p
Generation of recombinant adenoviral vectors encoding neural cell adhesion molecules ncam1, ncam2 and l1cam
Authors: Fedotova V. Y , Cherenkova E. E , Islamov R. R , Rizvanov A. A
Published: October 2024

To succed in gene therapy it is necessary to observe few basic conditions, such as targeted delivery of the therapeutic gene into the target organ and its effective expression. Nowadays targeted delivery of therapeutic genes is one of the critical problems of gene therapy. Delivery of recombinant genes using cell carriers (vectors), expressing tissue-specific cell adhesion molecules, allows us to move toward solving this problem. Using Gateway cloning technology (Invitrogen) we have created recombinant expression constructs pAd-NCAM1, pAd-NCAM2 and pAd-L1CAM, encoding neural cell adhesion molecules. Expression of recombinant proteins has been confirmed by immunofluorescent analysis. Based on these genetic constructs recombinant adenoviruses (serotype 5) were generated and titered. Obtained viral vectors encoding neural cell adhesion molecules may be subsequently used to modify cells carrying additional therapeutic genes to increase the efficiency of gene-cell therapeutics delivery to target organ.
Tissue-engineered constructions for skeletal muscle tissue repair
Authors: Korsakov I. N , Samchuk D. P , Eremin I. I , Zorin V. L , Deev R. V , Pulin A. A
Published: October 2024

Present review covers different types of tissue engineered constructions used for substitution of muscle tissue defects. Main methods of manufacturing of decellularized muscle scaffolds, mechanisms of action and results of their application in experiments in vivo are reviewed.
Skin Tissue Responses to an Artificial Material TIOPROST Developed for Use in Tissue Engineering
Authors: Rudneva A A , Sakharov A V , Makeev A A , Prosenko A E , Lukanina S N , Ryabchikova E I
Published: October 2024

Skin tissue reaction to implantation of synthetic material «TIOPROST» have been examined in 150 mice by morpho-histochemical analysis. Fast wound healing and absence of appreciable inflammatory reaction were observed in tissues surrounding the material during 3-40 days post implantation. The porous structure and chemical properties of the material modulated development of inflammation and provided formation of «a scaffold» for vessel growth inside the implant. Our study showed that the matrix based on «TIOPROST» is capable to fill in volume defects, to improve differentiation of fibroblastic cells, and to induce active migration of low-differentiated cells from stem cell niches, their proliferation and differentiation. The elaborated synthetic material «TIOPROST» can serve as osteoplastic material and the carrier of cells for development of tissue engineering constructions.
INTELIGÊNCIA ARTIFICIAL E O DIREITO DIGITAL
Authors: Mattos Ary Eduardo Nioac Prado de , Curto Leonardo Vilela , Mussallam Mariana Soares
Published: October 2024

Este artigo analisa, de forma aprofundada, os impactos da Inteligência Artificial (IA) no âmbito do Direito Digital e na sociedade moderna, com enfoque nas implicações de ordem jurídica, ética e regulatória. Quanto à legislação pátria e internacional, o estudo ressalta a premente necessidade de normativas específicas em razão da inadequação e insuficiência dos diplomas legais vigentes, que apenas delineiam diretrizes e princípios para lidar com as nuances e complexidades desta tecnologia disruptiva, complementando-o com uma abordagem histórico-filosófica ao revisitar o conceito de IA, desde René Descartes e Julien Offray de La Mettrie (século XVII) para traçar uma linha evolutiva que parte de seus primórdios até o estágio atual, marcada por inovações como o aprendizado de máquina, redes neurais artificiais e inteligência generativa. O trabalho também realiza uma exegese das consequências jurídico-sociais advindas da automatização de atividades intelectuais, evidenciando os reflexos dessa transformação, especialmente no tocante ao desemprego estrutural e à substituição da força de trabalho humano em funções cognitivas. Em paralelo, estão expostos os desafios regulatórios enfrentados pelo Direito Digital, que deve acompanhar o desenvolvimento tecnológico acelerado enquanto equilibra o incremento da produtividade com a mitigação dos impactos sociais negativos, garantindo a tutela dos direitos fundamentais e da segurança jurídica no ambiente digital, sob pena de se tornarem obsoletos.
Identification of Animals that Have the Potential as Seed Dispersers in the West Lampung Biodiversity Park
Authors: Putri Desma Ramadhina , Kanedi Mohammad , Priyambodo Priyambodo , Agustrina Rochmah
Published: October 2024

West Lampung Biodiversity Park is a biodiversity conservation area established in 2015. The purpose of establishing a biodiversity park is to provide a reserve area, both in-situ and/or ex-situ, for local biological resources, especially plants whose structure and composition can support the existence and sustainability of animal diversity. One group of animals that has an important role in the sustainability of flora biodiversity is seed disperser. This research was conducted to find out the current biodiversity status of animals having potential as seed dispersers. The research was carried out in September–November 2021 using interview and observation methods. The data was analyzed descriptively. The results show that in the West Lampung Biodiversity Park, 10 types of animals have the potential to spread seeds, consisting of 4 members of the aves class and 6 members of the mammal class. There are 4 types of fruit spread by Aves and 17 types of seeds spread by Mammals
Treatment of Songgo Langit Extract (Tridax procumbens) Against Blood Glucose Levels and Pancreatic Histopathology of Hyperglycemic Mice
Authors: Khoirunisa  , Nurcahyani Nuning , Ernawiati Eti , Widiastuti Endang Linirin
Published: October 2024

Hyperglycemia is an early symptom of diabetes caused by the pancreas not being able to produce enough insulin. This condition can disrupt blood glucose metabolism, thereby damaging organs in the body such as the pancreas. Hyperglycemia can be treated using traditional plant medicines. Songgo Langit leaves (Tridax procumbens L.) are reported to have secondary metabolite compounds that have potential as antidiabetic drugs. This study aims to determine the effect of administering Songgo Langit leaf extract on blood glucose levels and changes in the histopathological structure of the pancreas in hyperglycemic mice induced by alloxan. Using Complete Random Design, mice were divided into 5 groups of 5 mice each. Group K (-) was a negative control (not treated), group K (+) was a positive control (only induced by alloxan), group P1 was induced by alloxan and extract of 0.45 mg/g BW, group P2 was induced by alloxan and extract 0.9 mg /g BW, and group P3 was induced by alloxan and extract 1.8 mg/g BW for 14 days. Glucose level data were analyzed using ANOVA and post hoc test with LSD. Pancreatic histopathological structure data were analyzed using the Kruskal-Wallis test and the Mann-Whitney post hoc test. The results showed that the treatment of ethanol extract of Songgo Langit leaf at doses P1, P2, and P3 were significantly able to reduce blood glucose levels by 61.70%, 65.11%, and 74.79% and was able to repair damage to pancreatic histopathology of mice induced by alloxan.
Stomata Diversity Profile of Several Coffee Species at the Indonesian Coffee and Cocoa Research Center
Authors: Hapsari Tasya , Munandar Kukuh , Wibowo Ari
Published: October 2024

Stomata are a special part of plants, namely a number of leaf epidermal cells, both the upper and lower surface epidermis of the leaves. Stomata function as a place for CO2 to enter from the air in the process of photosynthesis, where respiration and transpiration take place so that stomata are an important tissue in plant metabolic processes. Observing stomata using a microscope is an effective and easy way to determine the type of stomata in angiosperm plants. This research was conducted on November 19 2022 with the aim of determining the type and diversity of stomata on several types of coffee plants at the Indonesian Coffee and Cocoa Research Center. The method used in this research is an exploratory survey method, while the replica method is used to look at leaf stomata, namely applying nail polish to the leaf surface and identifying the type of stomata using a qualitative descriptive method. The survey found that there were 9 types of coffee plants at the Indonesian Coffee and Cocoa Research Center. The results of the research show that the type of stomata on coffee plants based on the number and arrangement of neighboring cells is classified as a parasitic type. This can be seen from the presence of two neighboring cells surrounding the guard cell and the location of these cells parallel to the guard cell.
KETERKAITAN JUMLAH DAERAH TERMUTASI PADA GEN β-GLOBIN DENGAN INDEKS KORPUSKULAR PEMBAWA SIFAT β-THALASSEMIA
Authors: Priyambodo Priyambodo
Published: October 2024

Thalassemia is a genetic disorder caused by point mutations on the globin gene that decrease the corpuscular index in thalassemia carriers. Three to five percent of Indonesians are thalassemia carriers, with β-thalassemia being the most common type. This research aimed to identify the relationship between the number of mutated regions on the β-globin gene and the decrease in corpuscular index among β-thalassemia carriers. Data was collected from 2012 to 2013 in Yogyakarta. Hematological analysis was performed by assessing the corpuscular index, including mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) at Prodia Laboratory. Molecular analysis was conducted using the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method at the Laboratory of Genetics and Laboratory of Falitma, Biology Faculty, University of Gadjah Mada. Out of a total of 96 individuals screened, there were 9 suspected β-thalassemia carriers with 1 mutated region of the β-globin gene, showing an average MCV of 63.1 fl, MCH of 19.76 pg, and MCHC of 32.34 g/dl. Seven suspected β-thalassemia carriers with 2 mutated regions showed an average MCV of 61.16 fl, MCH of 19.74 pg, and MCHC of 32.3 g/dl. One suspected β-thalassemia carrier with 3 mutated regions showed an average MCV of 64.2 fl, MCH of 19.5 pg, and MCHC of 30.4 g/dl. The number of mutated regions in the β-globin gene was not the main factor affecting the decrease in corpuscular index among β-thalassemia carriers.
The Ukrainian Project “Re-vision of History” (2019): Identity Change and Memory Transformation as the Main Instruments of Cognitive Wars against Russia
Authors: Nina Ishchenko
Published: October 2024

The paper analyzes the cultural project of the Ukrainian public organization Internews-Ukraine, implemented in 2019 with the support of European foundations by cultural fi gures integrated into the European educational space. The cultural analysis of the collective memory of the Russians in Ukraine as a social group shows that the project is aimed at transforming Russian cultural identity by inverting key topoi of collective memory and replacing them with an ideological meta-narrative based on anti-Russian rhetoric. The analysis of deconstruction of narratives in the “Re-vision of History...”project shows that contradictory rhetorical strategies and manipulations of consciousness are used to infl uence the audience. The purpose of infl uencing the Russians of Ukraine through such projects is to legitimize the political power of Ukraine, which implements anti-Russian policy. Russian language abolition in Ukraine and the prohibition of contemporary Russian culture in the country cut off the Russians of Ukraine from the Russian cultural memory and include them in the Ukrainian cultural space, built on the interpellation of Russians as a marginal and subordinate group in society. It is noted that individual strategies for preserving Russian identity in Ukraine are insuffi cient, since identity is always transmitted and preserved with the group whose collective memory it forms
Socio-Cultural Self-identifi cation, Socio-Cultural Dynamics and the Politics of Memory (Russian Origin)
Authors: Chernyakhovsky Sergey
Published: October 2024

The author addresses the issues of the legitimizing and rehabilitative signifi cance of the memory policy and its role in the knowledge of one's own past in its positive and active meaning. With all the importance of the memory policy of the past, still even more important seems the memory policy of the future. It is noted that the combination of material and geographical conditions of the formation of Russia from ancient times gave rise to a type of historical practice that created such a spiritual tradition of Russia as a kind of confrontation between the found truth and the search for truth. Accordingly, such principles as Messianism, egalitarianism, and radicalism are tightly woven into the Russian tradition. It is concluded that the Russian consciousness swears allegiance to the state only to the extent to which it has sworn allegiance to a certain «Higher Idea», and to what extent this state has taken the same oath. In Russia the bearer of power exists only insofar as it embodies, on the one hand, the people's will, on the other, the idea accepted by the people. And abandoning this idea or not having it, denies him the right to power in the public consciousness.
“There was once such a word…”: the Development of “Serving” Concept in the 20th and Beginning of the 21st Centuries
Authors: Artem Boronin
Published: October 2024

The concept of “serving” while being one of the generative axiological foundations of Russian state system, is noted for its complicated development history in the 20th and the beginning of the 21st century. The present research is devoted to the analysis of the said process: how the concept of “serving” underwent changes from the time of Orthodox monarchic culture, through Soviet epoch and to the present stage. The present phenomenon is viewed in the context of the identity politics and historical memory which allows to examine the specifi cs of mutual state and public infl uence in the process of the spiritual and political order transformation
Protestant Mission and Russian Orthodoxy: Certain Aspects of YMCAQ Activities in Russia in the Beginning of the 20th Century
Authors: Shchipkova Lyubov
Published: October 2024

In the article the author analyzes the activities of YMCA American Protestant organization in the territory of the Russian empire before the revolution of 1917. Missionary activities were completed with the social aspects. YMCA activities were based on the social evangelism, “New Theology” and “muscular” Christianity trends. The ideas of Christian faith accommodation to the standards of modern life and culture were brought to the Orthodox grounds and contributed to the formation of views on the basis of modernism and liberalism. American Protestants activities were strongly supported by the Provisional Government and continued until the establishment of Soviet power in the territory of Russia. In the article the author pays special attention to the transition period with epoch destruction and to YMCA role in that process, as it primarily worked with the most active social segment – the youth.
Pelatihan dan Pendampingan Pengelolaan Usaha Domba di Yayasan Rumah Yatim Indonesia
Authors: Mutaqin Bambang Kholiq , Mansyur Mansyur , Ramdhani Diky
Published: October 2024

Yayasan Rumah Yatim Indonesia (RYI) resmi menjadi Laznas, sehingga kini mengelola dana zakat dan memajukan ekonomi umat melalui beberapa program usaha ekonomi produktif. Beberapa program ekonomi produktif yang dilaksanakan diantaranya mencakup bidang peternakan yaitu peternakan domba untuk qurban dan aqiqah. Fakultas Peternakan Unpad melaksanakan pelatihan dan pendampingan di RYI dalam program pengabdian pada masyarakat (PPM) melalui pelatihan dan pendampingan kepada pengelola RYI guna meningkatkan pengetahuan dan manajemen praktis peternakan domba. Pengabdian pada masyarakat dilaksanakan dari bulan Juni sampai Desember 2023. Pelatihan dan pendampingan bertujuan memberikan pengetahuan dan praktek peternakan domba kepada pengelola RYI, karena pengelola memiliki latar belakang bukan dari bidang peternakan dan pengetahuan dalam peternakan masih sedikit. Metode pelaksanaan berupa pemberian materi, diskusi, praktek, serta kunjungan ke praktisi peternakan yang sudah memiliki peternakan yang cukup besar. Hasil dari pelatihan dan pendampingan yaitu peningkatan pengetahuan bidang peternakan dan sudah mulainya pembangunan peternakan dimulai dari persiapan lahan kandang dan persiapan lahan rumput untuk pakan domba.
Cost Calculation and Selling Price Determination for MSME in Jababeka Industrial Area
Authors: Mapuasari Supeni Anggraeni , Fitriana Vita Elisa , Oktapriana Chita
Published: October 2024

Besides the position as an industrial center, Jababeka is also a residential and MSME center that supports the regional economy. Even though they are continuouesly growing, MSMEs still need a numeruos supports to be able to develop further, including how MSMEs can accurately calculate business costs and ultimately determine the right selling price. This training was divided into two sessions, where the first session focused on the concept of business costs, while in the second session, participants were asked to directly applied the previous concept and calculate their real business costs. The results of the questionnaire distributed to all 60 MSME participants showed that this training was able to provide an understanding of cost calculations and determining product selling prices. Therefore, it can be concluded that this training has successfully contributed to the MSME growth.
Fungsi Aplikasi Hornet Bagi Kalangan Gay di Kota Padang
Authors: Pratama Habil Agus , Erianjoni Erianjoni
Published: October 2024

Penelitian ini mengkaji serta menganalisis fungsi aplikasi hornet bagi kalangan gay yang berada di Kota Padang, yang merupakan kota dengan norma sosial yang kuat serta mayoritas masyarakatnya yang memegang teguh nilai-nilai religius. Penelitian ini bertujuan untuk menjelaskan fungsi aplikasi hornet dalam kehidupan sehari-hari penggunanya yang dianalisis dengan teori struktural fungsional dari Robert K. Merton. Menggunakan pendekatan kualitatif, penelitian ini melibatkan wawancara mendalam dan observasi partisipasi dengan informan kunci yang dipilih dengan teknik snowball sampling untuk memperoleh data penelitian yang akurat. Informan dalam penelitian ini terdiri dari 7 orang mahasiswa yang berada di Kota Padang yang berlangsung selama 3 bulan dengan menggunakan teknik analisis data yaitu reduksi data, penyajian data, dan penarikan kesimpulan. Temuan penelitian ini menunjukkan bahwa aplikasi hornet tidak hanya berfungsi untuk wadah berinteraksi sesama gay tetapi juga digunakan untuk memenuhi kebutuhan finansial, mencari pasangan dan wadah untuk mengekspresikan diri.
Strategi Pengelolaan Sampah di Kafe Rantiang Kota Padang
Authors: Fadila Shanista , Anzusi Syannajma , Effendi Aisyah , Aidil Muhammad , Wulandari Eka Pratiwi , Untari Elbunia , Emilza Lovia Phica Yola , Prayoga Ferry Ferdian , Gea Reri Damai , Nugraha Muhammad Ridho , Wahyuni Ameydea Nistrail
Published: October 2024

Artikel ini mengkaji tentang strategi dan inovasi dalam mengelola sampah di Kafe Rantiang yang berlokasi di Jalan Parkit, Kota Padang. Penelitian ini dilakukan melalui observasi langsung, wawancara dengn pemilik kafe serta studi literatur untuk menemukan strategi dan inovasi yang tepat untuk mengatasi permasalahan yang ditemukan. Tujuan penulisan ini untuk menggali strategi dan inovasi yang dapat dilakukan untuk mengelola sampah terutama sampah yang ada di Kafe Rantiang dalam meminimalkan dampak lingkungan dari limbah sampah yang dihasilkan.  Penelitian  ini  memberikan  wawasan  mendalam  tentang  bagaimana  strategi  dan inovasi dapat menjadi kunci dalam menciptakan model pengelolaan sampah yang berkelanjutan di sektor usaha kuliner. Implikasi temuan ini dapat memberikan inspirasi dan panduan bagi pelaku usaha serupa serta pemerintah daerah dalam upaya mencapai tujuan pembangunan berkelanjutan.
Role of Social Determinants of Health in Reproductive Cancer Care Among Women: A cross-sectional survey from diverse demographic and regional settings in India
Authors: Gadiraju Padmaja , Vanlalhruaii C , Dehury Ranjit Kumar , Singh Punam , Ahmad Imteyaz , Dehury Parthsarathi
Published: October 2024

Access to reproductive cancer care in India is significantly affected by social determinants of health. Reproductive cancers, including cervical, ovarian, and uterine cancers, constitute a significant health challenge for women in India. This cross-sectional survey aims to comprehensively assess the landscape of reproductive cancer care among women in India, exploring access, awareness, and barriers to timely diagnosis and treatment. This study highlights challenges in delivering care for reproductive cancers among women in India. A cross-sectional survey design was adopted, involving a representative sample of women from four diverse geographical regions, urban and rural settings, and varying socioeconomic backgrounds. Structured interviews were conducted, and questionnaires were used to collect data on participants’ awareness of reproductive cancers, utilization of healthcare facilities, and experiences related to access to cancer care. The study adopted a convenient sampling approach and captured data from 509 women diagnosed with reproductive cancer. The collected data were analysed using the SPSS 25 version. Univariate, bivariate and multivariate analyses were performed. The survey identified one-fourth of the respondents as having ovarian cancer, and 23.4% were diagnosed with cervical cancer. One-third of the study respondents were diagnosed with breast cancer, and 18% had other reproductive system cancers. The survey also assesses the prevalence of risk factors contributing to the incidence of reproductive cancers. Socioeconomic status, education level, rural‒urban settings, and cultural beliefs influence the healthcare-seeking behavior of the study participants. Insufficient knowledge, the stigma of reproductive health problems, and a lack of family income frequently discourage women from obtaining timely medical treatment.
When and WHY do we pretend (not) to understand: an attempt to MAKE a classification on the basis of meta-communicative statements
Authors: Mustajoki A.
Published: October 2024

According to the general rules of communication, interlocutors must conduct a conversation honestly. As you know, this is not always the case. The article deals with one type of deviation from this norm - situations in which the speaker (not) pretends that (does not) understand. Understanding is the key concept of communication, because in communication between people, not always but most often, interlocutors strive for mutual understanding. Thus, the task of the recipient is to identify the moments in which he does not understand what the speaker wants to express. If the recipient does not do this, he violates the general postulates of communication. When considering such cases, three main cases can be distinguished depending on who is the agent of speech, the recipient of the speech, the narrator, and the experiencer (the person who does not understand). Each of these situations has its own characteristics in relation to the reasons for using such a non-cooperative communication technique. To investigate this phenomenon, the article uses an unusual technique. The conclusions are based on the metalinguistic statements of the witnesses of the communication. The analysis showed that the experimenter pretends that he (does not) understand, in particular: (1) when he has a fundamental attitude to this means of communication (for example, (not) knowing a foreign language); (2) when (mis)understanding facilitates his position in a given situation (for example, when he is accused of something); (3) when (mis)understanding of what was said frees him from additional work (for example, when asking for advice); (4) when (mis)understanding occurs simply by inertia, from laziness or reluctance of the experimenter. The narrator, for his part, pretends not to understand most often when he condemns or criticizes the behavior of the experimenter.
The review of collective monograph "General and Russian linguoaxiology"(M.S. Milovanova (ed.). Moscow, Yaroslavl, Kantsler publ., 2022, 390 p.)
Authors: Radbil T.B.
Published: October 2024

The review highlights the main scientific ideas of the collective monograph "General and Russian linguoaxiology", prepared by scientists from the Institute of Linguistics of the Russian Academy of Sciences and Pushkin State Russian Language Institute. The authors of the monograph focus on the problems of such a modern interdisciplinary trend in the science of language as theoretical and applied linguoaxiology. The authors consistently consider axiology in vocabulary, grammar and discourse. The study is based on the category of value. The collective monograph reveals "priority directions of thought of a modern native speaker of the Russian language, his value orientations based on the study of the facts of the language" (p. 8). The practical value of the publication, which is addressed to linguist researchers, teachers, graduate students, and students of humanitarian faculties, is also undoubted.
Uygur Yazı Dili ve Sözlükçülüğü
Authors: Koraş Hikmet
Published: October 2024

Uygur yazı dili, tarihî Çağatay yazı dilinin devamı olan genç bir yazı dilidir.
Çağatay yazı dili, Doğu Türkistan dışında 20. yüzyılın ilk çeyreğinde
yerini yeni yazı dillerine bırakırken Doğu Türkistan’da 1950’lere kadar
mahallîleşerek devam etmiştir. 1950 ila 1980 yılları arası, oluşmakta olan
yeni Uygur yazı dili için bir belirsizlik dönemidir. Bu belirsizliğin sebebi,
bir taraftan alfabe değişikliği tartışmaları diğer taraftan da Çin’de
başlayan “Kültür Devrimi”dir. Kültür Devrimi’nden sonra kabul edilen
Latin alfabesinin ömrü sadece dokuz yıl sürmüş ve Uygur Türklüğü,
1983 yılında tekrar Arap alfabesini kullanmaya başlamıştır. Bu otuz yıllık
belirsizlik dönemi, Uygur yazı dilinin mevcut durumunda etkili olmuştur.
Doğu Türkistan’da kullanılan dille ilgili çalışmalar, 1800’lü yılların
sonuna rastlar. Ancak bu dönemde yapılan çalışmalar başlangıçta Çin
Cumhuriyeti ve Çarlık Rusyası hükûmetlerinin, ilerleyen zamanda Çin
Halk Cumhuriyeti ve Sovyetler Birliği’nin Doğu Türkistan bölgesinde ve
Uygurlar üzerinde uygulayacağı politikalara esas teşkil etmiştir. 1980’li
yıllarda Uygurlar arasında yoğun bir şekilde başlayan dil bilgisi ve sözlük
çalışmaları, bu döneme kadar yabancılar tarafından yapılan çalışmaların
esas alınmasıyla ortaya çıkmış olup bu çalışmaların amaçları pek
sorgulanmamıştır. Bu konuda Avrupa’da yapılan çalışmalarda bölgeden
“Eastern Turkestan”, dilden “Türkçe” yapılan derlemelerden “diyalekt”
şeklinde bahsedilirken Sovyetler Birliği sınırları içinde yapılan çalışmalarda
bölgeden Uygur Özerk Bölgesi, dilden Uygurca şeklinde bahsedilmesi
dikkat çeker. Türkiye’de konu ile ilgili çalışanlar da Sovyetler Birliği
bilim adamlarının kullandıkları terminolojiyi tercih etmişlerdir. Ayrıca
Türkiye’ye 1960’lı yıllarda Doğu Türkistan’dan Uygur kökenli insanların
geldiği dikkate alınırsa bu konuda 1990’lı yıllara kadar ciddi çalışmaların olduğu söylenemez. 1980’li yıllarda bir sağanak yağmur gibi ardı ardına
yayımlanan bilimsel çalışmaların sebeplerinden birisi, Çin hükûmetinin
dışa açılma çabası diğeri eskiden beri Türk dünyasının kültür merkezi
konumunda bulunan bölge insanının bilinçlenmesidir. Eserlerin çoğu, Çin
hükûmetinin kontrolündeki kurumlar aracılığı ile olsa da kısa sürede binlerce
eserin verilmesi bölge insanının okuma yazmaya yatkınlığı ve düşkünlüğü
ile izah edilebilirken yeni yazı dili de gelişme seyrine girmiştir.
Kazan Tatar Türkçesiyle Yazılmış Manzum Bir Tecvit Örneği: Fetḥü l-Tecvīd
Authors: YAVİ Fecri
Published: October 2024

Kur’an’ın doğru okunması esasına dayanan tecvit, İslami ilimler içerisinde
önemli bir yere sahip olmakla beraber öğrenilmesi pek kolay olmayan
bir ilimdir. Bu yüzden tecvidi daha akılda kalır, daha ilgi çekici ve özellikle
çocukların sevebileceği bir hâle getirmek için edebiyat tarihimizde pek
çok manzum tecvit örneği bulunmaktadır. Tecvidi öğrenmek isteyenlerin
bu manzum örneklerle daha hızlı ilerleyebileceği ve sıkılmadan öğrenmeye
devam edebileceği düşünülmüştür. Tecvidin sanatla bir arada sunulması
da öğrenme çağındaki çocuklar için edebî ve sanatsal becerinin
kazanılması için son derece önemlidir. Ünlü Kazan Tatar aydını, ulema ve
düşünürlerinden Alimcan Barudî ile kardeş olan Salihcan Barudî tarafından
yazılmış olan Fetḥü’l-Tecvīd, 1895’te Kazan’da basılmıştır. Kırgızistan
Millî İlimler Akademisi Beşeri ve İktisadi İlimler Bölümü Cengiz Aytmatov
Dil ve Edebiyat Enstitüsünün el yazmaları bölümünde kayıtlı olan
Fetḥü’l-Tecvīd, manzum bir tecvit örneği olması bakımından Kazan Tatar
edebiyatı için önem arz etmekte ve çoklu dil kullanımlarına sahip olması
onun tarihî Kazan Tatar Türkçesinin güzel bir örneği olduğunu göstermektedir.
Nitekim birtakım Oğuzca özellikler ve Çağatay imlasının esere
tatbik edildiği örnekler barındırdığı için dil özellikleri açısından eserin
günümüz Kazan Tatar Türkçesinden farklı yanları bulunmaktadır. Bu çalışmada
Fetḥü’l-Tecvīd transkribe edilerek günümüz Kazan Tatar Türkçesinden
farklı olan dil özellikleri üzerinde durulmuştur. Böylelikle Kazan
Tatar tasavvufi edebiyatının güzel bir örneği gün yüzüne çıkarılmış ve
tarihî Kazan Tatar Türkçesinin dil özellikleri aydınlatılmaya çalışılmıştır.
“Gün Olur Asra Bedel” ve “Ölüm Hükmü” Romanlarında Mankurtlaşma Kavramı Üzerine Bir İnceleme
Authors: sheıshenbekova Akmaral
Published: October 2024

Türk dünyası edebiyatının önde gelen yazarlarından Cengiz Aytmatov ve
Elçin Efendiyev’in hayatını ve edebî kişiliğini araştırırken birçok ortak
yön tespit ettik. Hem Aytmatov’un hem de Elçin’in çok dilli yazar ve bir
siyasetçi olarak yetişmelerinde aileleri, özellikle babaları büyük rol oynamıştır.
Babaları Törökul Aytmatov ile İlyas Efendiyev, Kırgız ve Azerbaycan
Türklerinin tarihinde büyük önem taşıyan aydınlardandır. Ancak her
iki yazarın da babalarının şöhretine sığınarak yükseldiklerini söyleyemeyiz.
Cengiz Aytmatov, Elçin’in ve Anar’ın babalarından etkilenip etkilenmedikleri
hakkında fikrini şu şekilde belirtmiştir: “Hem Elçin’in hem de
Anar’ın edebî kişiliğinin oluşumunda Azerbaycan’ın manevi ortamının
yanı sıra 20. yüzyıl Azerbaycan edebiyatının klasik yazarları ve babaları
Resul Rıza ve İlyas Efendiyev’in rolü büyüktür.” Cengiz Aytmatov ile
Elçin Efendiyev aynı dönemde yaşadıkları için aynı siyasi baskıların ve
toplumsal değişikliklerin şahidi olmuşlardır. Bu sebeple kaleme aldıkları
eserlerinde işlenen konularda pek çok ortaklık söz konusudur. İki yazarın
da kitapları Türkiye Türkçesi, Fransızca, İngilizce, Almanca gibi dünya
dillerine aktarılmıştır ve çeşitli ödüllere layık görülmüştür. Yazarların bazı
eserleri filme uyarlanmıştır. Günümüzde her iki yazar, Türk dünyasında
en çok okunan yazarların liste başında yer almaktadır. Bu makalede karşılaştırmalı
edebiyat kuralları dâhilinde Cengiz Aytmatov’un Gün Olur
Asra Bedel ve Elçin Efendiyev’in Ölüm Hükmü romanlarında geçen mankurtlaşma
kavramı incelenmeye ve mankurtlaşmanın Türk dünyasındaki iz düşümleri tespit edilmeye çalışılmıştır. Bu çalışmanın amacı, mankurtlaşma
kavramının Türk dünyası edebiyatında nasıl ele alındığı hakkında
söz konusu romanlar üzerinden tespitlerde bulunmaktır.
Problems and prospects of methodological features of accounting and audit of import operations
Authors: Saparbayeva Saule , Nabiyeva Akbayan , Christauskas Ceslovas
Published: October 2024

In the context of globalization and integration of world markets, import operations occupy an important place in the activities of enterprises, forming a significant part of their economic processes. Effective management of these operations requires accurate and timely accounting and high-quality auditing, which becomes especially relevant in modern conditions. The study of methodological features of accounting and auditing of import operations is a relevant and timely area that helps improve the quality of financial reporting and management decisions. The purpose of the study is to analyze the problems and prospects of methodological features of accounting and audit of import operations, as well as to develop recommendations for their improvement. The study examined the main methodological approaches, existing problems and challenges, and proposed solutions aimed at increasing the efficiency and reliability of accounting and auditing in a global economy. The improvement of methodological approaches to the accounting and auditing of import operations will improve the accuracy and reliability of financial reporting, reduce the risks of non-compliance with regulatory requirements, as well as improve management decision-making and the overall financial stability of companies. The development and implementation of effective accounting and auditing methods that comply with international standards and best practices will minimize financial risks and increase the competitiveness of enterprises in the global market. A study of the problems and prospects of methodological features of accounting and auditing of import operations has revealed a number of key issues that require attention and solutions. The main challenges are the complexity and diversity of regulatory requirements, currency fluctuations, the diversity of imported goods and services, difficulties in assessing and recognizing imported goods, and the lack of qualified specialists.
The role of transportation connectivity in Vietnam’s trade with ASEAN countries
Authors: Tuyet Le Thi Anh
Published: October 2024

This article focuses on studying how transportation connectivity affects Vietnam’s trade with Association of Southeast Asian Nations (ASEAN) countries. By using a gravity model, the article applies fixed effects (FE) and random effects (RE) to analyze panel data on trade, GDP, tariffs, border effects, and indicators. The number represents Vietnam’s transport connectivity with ASEAN countries from 2004 to 2021. Research results show that transport connectivity hurts Vietnam’s trade with other countries. ASEAN. The article proposes solutions for the Government and Vietnamese export enterprises to promote intra-ASEAN trade in the direction of increasing the added value of Vietnam’s imported and exported goods within ASEAN countries and balancing between Developing intra-ASEAN and foreign trade.
A note on the nomenclatural representation of plant transcription factors, and deviations thereof
Authors: Teixeira da Silva Jaime A.
Published: October 2024

Plant transcription factors (TFs) are integral proteins associated with gene transcription. That ability arises after TFs bind to specific DNA sequences, such as promoters, responding positively or negatively to a multitude of endogenous and exogenous factors or stressors. For this reason, in plants, the vast majority of TFs are associated with growth, development, and responses to biotic and abiotic stresses. As is commonly known, the genes coding for TFs are written in uppercase italics, whereas the resulting proteins are written in lowercase, such as OsWRKY28 and OsWRKY28, respectively for a member of the WRKY TF family in rice (Oryza sativa L.). However, despite the existence of some conventions for the nomenclature of TFs, some deviation from these norms has been observed in the literature. Several cases are highlighted, primarily related to NAC TFs. These cases emphasize the need for stricter quality control of literature pertaining to the nomenclature of plant TFs.
Machine Learning-Driven Prediction of CRISPR-Cas9 Off-Target Effects and Mechanistic Insights
Authors: Bhardwaj Anuradha , Tomar Pradeep , Nain Vikrant
Published: October 2024

Background

The precise prediction of off-target effects in CRISPR-Cas9 genome editing is critical for ensuring the safety and efficacy of this powerful tool. This study leverages machine learning techniques to predict off-target cleavage sites and investigate the underlying mechanisms that affect cleavage efficiencies. By integrating data from Tsai et al. and Kleinsteiver et al., who employed the GUIDE-seq method, we aim to enhance our understanding of the factors influencing CRISPR-Cas9 activity.

Results

Our research analyzed datasets from Tsai et al. and Kleinsteiver et al., standardizing cleavage efficiencies to align with Tsai et al.’s comprehensive dataset. We identified a range of sequence features, including PAM sequence types, nucleotide composition, GC content, chromatin structure, CpG islands, and gene expression levels. Various machine learning models, including Artificial Neural Networks, Support Vector Machines, Naïve Bayes, k-Nearest Neighbors, Logistic Regression, and Extra Trees Classifiers, were developed and evaluated. The Extra Trees Classifier, particularly with class weighting, exhibited robust performance, achieving high accuracy, precision, recall, and F1 scores. SHAP analysis provided insights into feature importance, highlighting the significant factors contributing to model predictions.

Conclusions

The application of machine learning to predict CRISPR-Cas9 off-target effects demonstrates significant potential in enhancing the precision of genome editing. Our findings underscore the importance of considering a diverse range of sequence and genomic features to improve prediction models. The insights gained from this study can inform the development of safer and more effective CRISPR-based applications in medicine, agriculture, and biotechnology. Future work will focus on further refining these models and exploring their applicability across different genomic contexts.
The Role of Cejusc in Promoting Access to Justice and Creating An Environment Conducive to Community Participation in the Search for Consensual Solutions
Authors: Bedê Júnior Américo , Cruz Rômulo Lago e
Published: October 2024

Objective: The objective of this study is to investigate the importance of Judicial Centers for Conflict Resolution and Citizenship (Cejuscs) in expanding access to justice and modernizing the Brazilian judicial system, focusing on mediation and negotiation as alternatives to the traditional adversarial model.
 
Theoretical Framework: The research is based on theories of mediation and negotiation, including the Harvard method, and discusses the relevance of these techniques in the current legal context. Additionally, examples from New York's experience are used to enrich the analysis of Cejuscs' role.
 
Method: The methodology includes a bibliographic analysis of data on Cejuscs and a specific case study in the Vitorino Freire/MA District, where the implementation of a differentiated model focused on consensus building was proposed. The study also covers the structure and operation of Cejuscs, as well as the selection and training of mediators and conciliators.
 
Results and Discussion: The results indicate that Cejuscs are effective in promoting consensual alternatives for conflict resolution, and their role can be expanded to handle complex collective disputes. The experience in Vitorino Freire/MA highlights the importance of interinstitutional partnerships for the success of the model.
 
Research Implications: This study suggests that the Cejuscs model can be adapted to meet new challenges, such as mediating collective disputes, offering a more efficient and cost-effective path to conflict resolution.
 
Originality/Value: The research contributes by highlighting the need to modernize judicial practices in Brazil and proposes an innovative model for Cejuscs’ performance in highly complex contexts.