bluuebunny/crossref_metadata_2025 huggingface dataset:
Adaptive Hybrid Mixed Two-Point Step Size Gradient Algorithm for Solving Non-Linear Systems
Authors: Ali Eltiyeb , Mahdi Salem
Published: April 2023

In this paper, a two-point step-size gradient technique is proposed by which the approximate solutions of a non-linear system are found. The two-point step-size includes two types of parameters deterministic and random. A new adaptive backtracking line search is presented and combined with the two-point step-size gradient to make it globally convergent. The idea of the suggested method depends on imitating the forward difference method by using one point to estimate the values of the gradient vector per iteration where the number of the function evaluation is at most one for each iteration. The global convergence analysis of the proposed method is established under actual and limited conditions. The performance of the proposed method is examined by solving a set of non-linear systems containing high dimensions. The results of the proposed method is compared to the results of a derivative-free three-term conjugate gradient CG method that solves the same test problems. Fair, popular, and sensible evaluation criteria are used for comparisons. The numerical results show that the proposed method has merit and is competitive in all cases and superior in terms of efficiency, reliability, and effectiveness in finding the approximate solution of the non-linear systems.
An Inertial Forward–Backward Splitting Method for Solving Modified Variational Inclusion Problems and Its Application
Authors: Sombut Kamonrat , Sitthithakerngkiet Kanokwan , Arunchai Areerat , Seangwattana Thidaporn
Published: April 2023

In this paper, we propose an inertial forward–backward splitting method for solving the modified variational inclusion problem. The concept of the proposed method is based on Cholamjiak’s method. and Khuangsatung and Kangtunyakarn’s method. Cholamjiak’s inertial technique is utilized in the proposed method for increased acceleration. Moreover, we demonstrate that the proposed method strongly converges under appropriate conditions and apply the proposed method to solve the image restoration problem where the images have been subjected to various obscuring processes. In our example, we use the proposed method and Khuangsatung and Kangtunyakarn’s method to restore two medical images. To compare image quality, we also evaluate the signal-to-noise ratio (SNR) of the proposed method to that of Khuangsatung and Kangtunyakarn’s method.
Bursting Dynamics of Spiking Neural Network Induced by Active Extracellular Medium
Authors: Stasenko Sergey V. , Kazantsev Victor B.
Published: April 2023

We propose a mathematical model of a spiking neural network (SNN) that interacts with an active extracellular field formed by the brain extracellular matrix (ECM). The SNN exhibits irregular spiking dynamics induced by a constant noise drive. Following neurobiological facts, neuronal firing leads to the production of the ECM that occupies the extracellular space. In turn, active components of the ECM can modulate neuronal signaling and synaptic transmission, for example, through the effect of so-called synaptic scaling. By simulating the model, we discovered that the ECM-mediated regulation of neuronal activity promotes spike grouping into quasi-synchronous population discharges called population bursts. We investigated how model parameters, particularly the strengths of ECM influence on synaptic transmission, may facilitate SNN bursting and increase the degree of neuronal population synchrony.
Evaluation of Phytochemical Screening, Pigment Content, In Vitro Antioxidant, Antibacterial Potential and GC-MS Metabolite Profiling of Green Seaweed Caulerpa racemosa
Authors: Palaniyappan Sivagaami , Sridhar Arun , Kari Zulhisyam Abdul , Téllez-Isaías Guillermo , Ramasamy Thirumurugan
Published: April 2023

Exploration of seaweeds to unravel their bioactive metabolites from the perspective of wider applications gained substantial importance. The present study was performed to investigate the total phenolic, flavonoid, tannin content, antioxidant activity and antibacterial potential of various solvent extracts of green seaweed Caulerpa racemosa. The methanolic extract showed higher phenolic (11.99 ± 0.48 mg gallic acid equivalents/g), tannin (18.59 ± 0.54 mg tannic acid equivalents/g) and flavonoid (33.17 ± 0.76 mg quercetin equivalents/g) content than other extracts. Antioxidant activity was determined by using 2,2-diphenylpicrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay with different concentrations of C. racemosa extracts. The methanolic extract showed higher scavenging potential in both the DPPH and ABTS activity with the inhibition value of 54.21 ± 1.39% and 76.62 ± 1.08%, respectively. Bioactive profiling was also identified by using Gas chromatography-mass spectrometry (GC-MS) and Fourier transform infrared (FT-IR) techniques. These studies revealed the presence of valuable bioactive compounds in C. racemosa extracts and these compounds might be responsible for antimicrobial, antioxidant, anticancer and anti-mutagenic properties. Major compounds identified in GC-MS were 3,7,11,15-Tetramethyl-2-hexadecen-1-ol, 3-hexadecene and Phthalic acid. In terms of antibacterial activity, C. racemosa has promising antibacterial potential against aquatic pathogens Aeromonas hydrophila, Aeromonas veronii and Aeromonas salmonicida. Further evaluation studies focusing aquatic related aspects would reveal the novel bioproperties and applications of C. racemosa.
Marine Invertebrates: A Promissory Still Unexplored Source of Inhibitors of Biomedically Relevant Metallo Aminopeptidases Belonging to the M1 and M17 Families
Authors: Pascual Alonso Isel , Almeida García Fabiola , Valdés Tresanco Mario Ernesto , Arrebola Sánchez Yarini , Ojeda del Sol Daniel , Sánchez Ramírez Belinda , Florent Isabelle , Schmitt Marjorie , Avilés Francesc Xavier
Published: April 2023

Proteolytic enzymes, also known as peptidases, are critical in all living organisms. Peptidases control the cleavage, activation, turnover, and synthesis of proteins and regulate many biochemical and physiological processes. They are also involved in several pathophysiological processes. Among peptidases, aminopeptidases catalyze the cleavage of the N-terminal amino acids of proteins or peptide substrates. They are distributed in many phyla and play critical roles in physiology and pathophysiology. Many of them are metallopeptidases belonging to the M1 and M17 families, among others. Some, such as M1 aminopeptidases N and A, thyrotropin-releasing hormone-degrading ectoenzyme, and M17 leucyl aminopeptidase, are targets for the development of therapeutic agents for human diseases, including cancer, hypertension, central nervous system disorders, inflammation, immune system disorders, skin pathologies, and infectious diseases, such as malaria. The relevance of aminopeptidases has driven the search and identification of potent and selective inhibitors as major tools to control proteolysis with an impact in biochemistry, biotechnology, and biomedicine. The present contribution focuses on marine invertebrate biodiversity as an important and promising source of inhibitors of metalloaminopeptidases from M1 and M17 families, with foreseen biomedical applications in human diseases. The results reviewed in the present contribution support and encourage further studies with inhibitors isolated from marine invertebrates in different biomedical models associated with the activity of these families of exopeptidases.
Sexual Dysfunctions in Females with Parkinson’s Disease: A Cross-Sectional Study with a Psycho-Endocrinological Perspective
Authors: De Luca Rosaria , Bonanno Mirjam , Morini Elisabetta , Marra Angela , Arcadi Francesca Antonia , Quartarone Angelo , Calabrò Rocco Salvatore
Published: April 2023

Background and Objectives: Normal human sexual functioning is a complex integration of an intact neuroanatomic substrate, vascular supply, a balanced hormonal profile, and a predominance of excitatory over inhibitory psychological mechanisms. However, sexual functioning in Parkinson’s disease (PD) is often overlooked in clinical practice, especially in female patients. Materials and Methods: In this cross-sectional study, we have investigated the frequency of sexual dysfunction and the possible correlation with psycho-endocrinological factors in a sample of women with idiopathic PD. Patients were assessed using a semi-structured sexual interview, in addition to psychometric tools, including the Hamilton Rating Scale for Anxiety and for Depression and the Coping Orientation to the Problems Experiences—New Italian Version. Specific blood tests, including testosterone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), estrogen E2, prolactin (PRL), and vitamin D3 were also evaluated. Results: Our results reported a statistical difference in sexual intercourse frequency before and after the onset of PD (p < 0.001). The percentage of women who complained about reduced sexual desire increased after diagnosis (52.7%) compared to the period before the onset of the illness (36.8%). The endocrinological profile in females with PD revealed statistically significant differences regarding testosterone (p < 0.0006), estradiol (p < 0.00), vitamin D3 (p < 0.006), and calcium (0.002). Depression (44% characterized by perceived feelings of anger and frustration during sexual intercourse) and anxiety symptoms (29.5% reported feelings of fear and anxiety for not satisfying the partner) with abnormal coping strategies (48.14% experienced feelings of anger and intolerance) were also found to be statistically significant. This study showed a high frequency of sexual dysfunction in female patients with PD, which correlated with sexual hormone abnormalities, mood/anxiety, and coping strategies alterations. This supports the idea that there is a need to better investigate the sexual function of female patients with PD to provide them with an adequate therapeutic approach and potentially improve quality of life.
The Effect of Timing and Methods for the Diagnosis of Gestational Diabetes Mellitus on Obstetric Complications
Authors: Galdikaitė Gintarė , Simanauskaitė Atėnė , Ramonienė Gitana , Savukynė Eglė , Malakauskienė Laura , Tarasevičienė Viktorija
Published: April 2023

Aim. To compare the impact of the time and method of diagnosis on gestational diabetes mellitus (GDM) in women who gave birth at the Hospital of the Lithuanian University of Health Sciences (LUHS) Kauno klinikos. Methods. A retrospective study was performed using data from the Department of Obstetrics and Gynecology of the LUHS Birth Registry to analyze the data of women who gave birth and had GDM in 2020–2021. The subjects were divided based on the type of diagnosis: GDM was diagnosed either at the first antenatal visit when fasting plasma glycemia (FPG) was ≥5.1 mmol/L (early diagnosis group) or after OGTT at 24 + 0 − 28 + 6 weeks of gestation when at least one pathological glycemic index was observed: fasting glycemia 5.1–6.9 mmol/L or 1-h glycemia ≥10.0 mmol/L or 2 h glycemia 8.5–11.0 mmol/L (late diagnosis group). The results were processed using IBM SPSS. Results. The early diagnosis group had 1254 (65.7%) women, the late diagnosis group had 654 (34.3%). More primigravida women were in the late diagnosis group (p = 0.017) while more multigravida were in the early diagnosis group (p = 0.033). The early diagnosis group had more obese women (p = 0.001), including those with a BMI > 40 (p = 0.001). In the early diagnosis group, GDM was more frequently diagnosed in women who gained <11 kg (p = 0.005), while in the late diagnosis group—>16 kg (p = 0.001). FPG was higher in the early diagnosis group (p = 0.001). Glycemia was more commonly corrected with lifestyle changes in the late diagnosis group (p = 0.001), and with additional insulin therapy in the early diagnosis group (p = 0.001). Polyhydramnios and preeclampsia were more common in the late diagnosis group (p = 0.027 and p = 0.009). There were more large-for-gestational-age neonates in the late diagnosis group (p = 0.005). Macrosomia was more common in the late diagnosis group (p = 0.008). Conclusions. GDM is more commonly diagnosed with OGTT in primigravida women. Higher pregestational weight and BMI has an impact on the early diagnosis of GDM and need for insulin therapy with lifestyle changes. Late diagnosis of GDM is connected with obstetric complications.
Host-Associated Bacteroides 16S rDNA-Based Markers for Source Tracking of Fecal Pollution in Laguna Lake, Philippines
Authors: Malajacan Gicelle T. , Nacario Mae Ashley G. , Obusan Marie Christine M. , Rivera Windell L.
Published: April 2023

Sources of fecal contamination in Laguna Lake, Philippines, were identified using a library-independent microbial source tracking method targeting host-associated Bacteroides 16S rDNA-based markers. Water samples from nine lake stations were assessed for the presence of the fecal markers HF183 (human), BoBac (cattle), Pig-2-Bac (swine), and DuckBac (duck) from August 2019 to January 2020. HF183 (average concentration = 1.91 log10 copies/mL) was the most frequently detected, while Pig-2-Bac (average concentration = 2.47 log10 copies/mL) was the most abundant. The detected marker concentrations in different stations corresponded to the land use patterns around the lake. Generally, all marker concentrations were higher during the wet season (August–October), suggesting the effect of rainfall-associated factors on the movement and retention of markers from sources. There was a significant association (ρ = 0.45; p < 0.001) between phosphate and the concentration of HF183, suggesting domestic sewage-derived pollution. The markers had acceptable sensitivity and specificity, i.e., HF183 (S = 0.88; R = 0.99), Pig-2-Bac (S = 1.00; R = 1.00), and DuckBac (S = 0.94; R = 1.00), and therefore may be used for the continuous monitoring of fecal pollution in the lake and in designing interventions to improve the quality of the lake water.
Antimicrobial Photodynamic Therapy against Escherichia coli and Staphylococcus aureus Using Nanoemulsion-Encapsulated Zinc Phthalocyanine
Authors: Felifel Nada T. , Sliem Mahmoud A. , Kamel Zienat , Bojarska Joanna , Seadawy Mohamed G. , Amin Rehab M. , Elnagdy Sherif M.
Published: April 2023

Multidrug-resistant microorganisms have become a significant public health threat, and traditional antibiotics are becoming ineffective. Photodynamic therapy (PDT) is a promising alternative that utilizes photosensitizers and light to produce Reactive Oxygen Species (ROS) that can kill microorganisms. Zinc phthalocyanine (ZnPc) is a promising photosensitizer due to its strong affinity for encapsulation in nanoemulsions and its antimicrobial properties. In this study, nanoemulsion was prepared using Miglyol 812N, a surfactant, and distilled water to dissolve hydrophobic drugs such as ZnPc. The nanoemulsion was characterized by its particle size, polydispersity index, Transmission Electron Microscope and Zeta potential, and the results showed that it was an efficient nanocarrier system that facilitated the solubilization of hydrophobic drugs in water. The use of ZnPc encapsulated in the nanoemulsion produced through the spontaneous emulsification method resulted in a significant reduction in cell survival percentages of gram-positive Staphylococcus aureus and gram-negative Escherichia coli by 85% and 75%, respectively. This may be attributed to the more complex cell membrane structure of E. coli compared to S. aureus. This demonstrates the potential of nanoemulsion-based PDT as an effective alternative to traditional antibiotics for treating multidrug-resistant microorganisms.
Impact of Water Shortage on Soil and Plant Attributes in the Presence of Arbuscular Mycorrhizal Fungi from a Harsh Environment
Authors: Silva Antonio Marcos Miranda , Feiler Henrique Petry , Qi Xue , de Araújo Victor Lucas Vieira Prudêncio , Lacerda-Júnior Gileno Vieira , Fernandes-Júnior Paulo Ivan , Cardoso Elke Jurandy Bran Nogueira
Published: April 2023

Arbuscular mycorrhizal fungi (AMF) play a crucial role in plant health due to their ability to improve tolerance to biotic and abiotic stresses. Our aim was to evaluate the effectiveness of a pool of native AMF from a harsh environment on plant performance and changes in soil attributes under different levels of drought. An experiment using maize was established, varying the soil water content to simulate severe drought (30% of the water-holding capacity [WHC]), moderate (50% of the WHC) and no drought (80% of the WHC, control treatment). Soil and plant attributes were measured (enzyme activity, microbial biomass, AMF root colonisation and plant biomass and nutrient uptake). There was a two-fold increase in plant biomass under moderate drought when compared to no drought treatment, but there was no difference in nutrient uptake. Under severe drought, there were the highest enzyme activities related to phosphorus (P) cycling and P microbial biomass, indicating higher P microbial immobilization. The increase in AMF root colonisation was observed in plants under moderate and no drought. Our findings demonstrated that the better use of the AMF inoculum varied according to drought levels, with better performance under moderate drought due to the increase in plant biomass.
Identification and Construction of Strong Promoters in Yarrowia lipolytica Suitable for Glycerol-Based Bioprocesses
Authors: Georgiadis Ioannis , Tsiligkaki Christina , Patavou Victoria , Orfanidou Maria , Tsoureki Antiopi , Andreadelli Aggeliki , Theodosiou Eleni , Makris Antonios M.
Published: April 2023

Yarrowia lipolytica is a non-pathogenic aerobic yeast with numerous industrial biotechnology applications. The organism grows in a wide variety of media, industrial byproducts, and wastes. A need exists for molecular tools to improve heterologous protein expression and pathway reconstitution. In an effort to identify strong native promoters in glycerol-based media, six highly expressed genes were mined from public data, analyzed, and validated. The promoters from the three most highly expressed (H3, ACBP, and TMAL) were cloned upstream of the reporter mCherry in episomal and integrative vectors. Fluorescence was quantified by flow cytometry and promoter strength was benchmarked with known strong promoters (pFBA1in, pEXP1, and pTEF1in) in cells growing in glucose, glycerol, and synthetic glycerol media. The results show that pH3 > pTMAL > pACBP are very strong promoters, with pH3 exceeding all other tested promoters. Hybrid promoters were also constructed, linking the Upstream Activating Sequence 1B (UAS1B8) with H3(260) or TMAL(250) minimal promoters, and compared to the UAS1B8-TEF1(136) promoter. The new hybrid promoters exhibited far superior strength. The novel promoters were utilized to overexpress the lipase LIP2, achieving very high secretion levels. In conclusion, our research identified and characterized several strong Y. lipolytica promoters that expand the capacity to engineer Yarrowia strains and valorize industrial byproducts.
Bacterial Microbiota of Asthmatic Children and Preschool Wheezers’ Airways—What Do We Know?
Authors: Bar Kamil , Litera-Bar Maja , Sozańska Barbara
Published: April 2023

Asthma is the most chronic pulmonary disease in pediatric population, and its etiopathology still remains unclear. Both viruses and bacteria are suspected factors of disease development and are responsible for its exacerbation. Since the launch of The Human Microbiome Project, there has been an explosion of research on microbiota and its connection with various diseases. In our review, we have collected recent data about both upper- and lower-airway bacterial microbiota of asthmatic children. We have also included studies regarding preschool wheezers, since asthma diagnosis in children under 5 years of age remains challenging due to the lack of an objective tool. This paper indicates the need for further studies of microbiome and asthma, as in today’s knowledge, there is no particular bacterium that discriminates the asthmatics from the healthy peers and can be used as a potential biological factor in the disease prevalence and treatment.
Prevalence of mecA and Panton-Valentine Leukocidin Genes in Staphylococcus aureus Clinical Isolates from Gaza Strip Hospitals
Authors: El Aila Nabil Abdullah , Al Laham Nahed Ali , Naas Thierry
Published: April 2023

Methicillin-resistant Staphylococcus aureus (MRSA) are spreading worldwide in hospital and community settings, thus posing a serious public health problem. Panton-Valentine Leukocidin (PVL), an important virulence factor of S. aureus, is a marker of community-acquired MRSA. Here we determined the prevalence of pvl genes among S. aureus isolates from different hospitals in the Gaza Strip, Palestine. A total of 285 S. aureus isolates were collected from five different hospitals in the Gaza Strip. All isolates were characterized for their susceptibility patterns to available antimicrobial agents and by using multiplex PCR for the detection of mecA and pvl genes. The overall prevalence of MRSA in Gaza hospitals was 70.2% (range: 76.3% to 65.5%) and that of pvl among S. aureus isolates was 29.8% (range: 32.9% to 26.2%). The pvl gene was equally prevalent among MRSA isolates (30.5%) and MSSA isolates (28.2%). The most effective antibiotics were rifampicin, vancomycin, and clindamycin, with susceptibility rates of 91.2%, 88.7%, and 84.6%, respectively. The highest percentage of strains were observed to be resistant to penicillin and amoxicillin with clavulanic acid—96.1% and 73.6%, respectively. Our results showed a high prevalence of MRSA and pvl-positive isolates in Gaza Strip hospitals, which likely reflects the situation in the community. It is mandatory to implement systematic surveillance of both hospital and community isolates, together with interventions (such as increased hand hygiene, use of hydroalcoholic solutions, and isolation of carriers) to limit their spread.
Insights into Antimicrobial and Anti-Inflammatory Applications of Plant Bioactive Compounds
Authors: Mitropoulou Gregoria , Stavropoulou Elisavet , Vaou Natalia , Tsakris Zacharias , Voidarou Chrysa , Tsiotsias Arsenis , Tsigalou Christina , Taban Birce Mercanoglou , Kourkoutas Yiannis , Bezirtzoglou Eugenia
Published: April 2023

Plants have long been thought to contribute to health promotion due to their fiber and phenolic content, as well as their inherent biological potential. The bioactive derivatives of medicinal plants are a valuable resource in the fight against serious diseases all around the world. The present review focuses on the current state of knowledge on the usage and medicinal applications of plant bioactives. Issues concerning the effect of aromatic plant derivatives on human gut microbiota and their antimicrobial and anti-inflammatory potentials are discussed and worth further exploring.
Nutritional Value and Antioxidant, Antimicrobial and Cytotoxic Activity of Wild Macrofungi
Authors: Dávila Giraldo Lina Rocío , Pérez Jaramillo Claudia Cristina , Méndez Arteaga Jonh Jairo , Murillo-Arango Walter
Published: April 2023

Macrofungi are among the most promising sources of biologically active natural products with nutritional qualities and therapeutic values. In this work, the nutritional value of nine species of wild macrofungi from Ibague-Tolima (Colombia) was evaluated. In addition the antioxidant, antimicrobial and cytotoxic activities of an ethanol:water (70:30) extract of wild basidiomata were evaluated. The wild mushrooms’ nutritional potential showed that the genus Pleurotus and Lentinus have the best protein percentages, with 18.4% and 18.5%. The nine extracts evaluated managed to stabilize the two radicals evaluated; however, lower IC50 was found for Phellinus gilvus and Ganoderma australe extracts. The results showed that Trametes coccinea, Pleurotus floridanus and Ganoderma australe extracts were the most effective as antimicrobials, with high inhibition percentages against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae. Antifungal activity results against Rhizopus oryzae, Penicillium sp. and Aspergillus niger showed that the nine extracts were effective at the concentrations tested. Considering cell viability against isolated leukocytes, seven of the nine extracts showed percentages higher than 50% of cell viability. This research describes the nutritional value of nine wild macrofungi in Colombia and their potential for antimicrobial, cytotoxic and antioxidant activity.
Latest Advances in Arbovirus Diagnostics
Authors: Varghese Jano , De Silva Imesh , Millar Douglas S.
Published: April 2023

Arboviruses are a diverse family of vector-borne pathogens that include members of the Flaviviridae, Togaviridae, Phenuviridae, Peribunyaviridae, Reoviridae, Asfarviridae, Rhabdoviridae, Orthomyxoviridae and Poxviridae families. It is thought that new world arboviruses such as yellow fever virus emerged in the 16th century due to the slave trade from Africa to America. Severe disease-causing viruses in humans include Japanese encephalitis virus (JEV), yellow fever virus (YFV), dengue virus (DENV), West Nile virus (WNV), Zika virus (ZIKV), Crimean–Congo hemorrhagic fever virus (CCHFV), severe fever with thrombocytopenia syndrome virus (SFTSV) and Rift Valley fever virus (RVFV). Numerous methods have been developed to detect the presence of these pathogens in clinical samples, including enzyme-linked immunosorbent assays (ELISAs), lateral flow assays (LFAs) and reverse transcriptase–polymerase chain reaction (RT-PCR). Most of these assays are performed in centralized laboratories due to the need for specialized equipment, such as PCR thermal cyclers and dedicated infrastructure. More recently, molecular methods have been developed which can be performed at a constant temperature, termed isothermal amplification, negating the need for expensive thermal cycling equipment. In most cases, isothermal amplification can now be carried out in as little as 5–20 min. These methods can potentially be used as inexpensive point of care (POC) tests and in-field deployable applications, thus decentralizing the molecular diagnosis of arboviral disease. This review focuses on the latest developments in isothermal amplification technology and detection techniques that have been applied to arboviral diagnostics and highlights future applications of these new technologies.
β-Barrel Assembly Machinery (BAM) Complex as Novel Antibacterial Drug Target
Authors: Xu Qian , Guo Min , Yu Feiyuan
Published: April 2023

The outer membrane of Gram-negative bacteria is closely related to the pathogenicity and drug resistance of bacteria. Outer membrane proteins (OMPs) are a class of proteins with important biological functions on the outer membrane. The β-barrel assembly machinery (BAM) complex plays a key role in OMP biogenesis, which ensures that the OMP is inserted into the outer membrane in a correct folding manner and performs nutrient uptake, antibiotic resistance, cell adhesion, cell signaling, and maintenance of membrane stability and other functions. The BAM complex is highly conserved among Gram-negative bacteria. The abnormality of the BAM complex will lead to the obstruction of OMP folding, affect the function of the outer membrane, and eventually lead to bacterial death. In view of the important role of the BAM complex in OMP biogenesis, the BAM complex has become an attractive target for the development of new antibacterial drugs against Gram-negative bacteria. Here, we summarize the structure and function of the BAM complex and review the latest research progress of antibacterial drugs targeting BAM in order to provide a new perspective for the development of antibiotics.
Computer Analysis of the Inhibition of ACE2 by Flavonoids and Identification of Their Potential Antiviral Pharmacophore Site
Authors: Bogoyavlenskiy Andrey , Alexyuk Madina , Alexyuk Pavel , Berezin Vladimir , Almalki Faisal A. , Ben Hadda Taibi , Alqahtani Alaa M. , Ahmed Saleh A. , Dall’Acqua Stefano , Jamalis Joazaizulfazli
Published: April 2023

In the present study, we investigated the antiviral activities of 17 flavonoids as natural products. These derivatives were evaluated for their in vitro antiviral activities against HIV and SARS-CoV-2. Their antiviral activity was evaluated for the first time based on POM (Petra/Osiris/Molispiration) theory and docking analysis. POM calculation was used to analyze the atomic charge and geometric characteristics. The side effects, drug similarities, and drug scores were also assumed for the stable structure of each compound. These results correlated with the experimental values. The bioinformatics POM analyses of the relative antiviral activities of these derivatives are reported for the first time.
Synthesis of New Liquid-Crystalline Compounds Based on Terminal Benzyloxy Group: Characterization, DFT and Mesomorphic Properties
Authors: Omar Alaa Z. , Alazmi Mohammed L. , Alsubaie Mai S. , Hamed Ezzat A. , Ahmed Hoda A. , El-Atawy Mohamed A.
Published: April 2023

The effect of the terminal benzyloxy group on the mesomorphic properties of liquid crystalline materials developed from rod-like Schiff base has been described. For this objective, a novel Schiff base liquid crystal family, specifically new series of Schiff base liquid crystals, namely, (E)-4-(alkyloxy)-N-(4-(benzyloxy)benzylidene)aniline, In, are prepared and investigated in detail. The length of the terminal alkyloxy chain (n) varies amongst the compounds in the series. Where n varies between 6, 8 and 16 carbons. At the other end of the compounds, benzyloxy moiety was attached. The molecular structures of all synthesized compounds were established using different spectroscopic techniques. The molecular self-assembly was explored using differential scanning calorimetry (DSC) and polarized optical microscope (POM). Depending on the length of the terminal alkyloxy chain, only one type of SmA phase with different stability was observed. The previously reported para-substituted systems and the present investigated compounds were compared and discussed. The calculated quantum chemical parameters were computationally correlated using the DFT method via the B3LYP 6-311G(d,p) basis set. The theoretical computations revealed that the length of the alkyl side chain influences the zero-point energy, reactivity and other estimated thermodynamic parameters of benzoyloxy/azomethine derivatives. Furthermore, the FMO energy analysis shows that molecule I16 have higher HOMO energies than the other compounds, and I6 has a much lower LUMO level than the rest.
The Influence of Nitrogen Applications and Low Rainfall Conditions on Yield of Mixed Grass-Legume Grassland for 2 Years
Authors: Weggler Karin , Elsäßer Martin
Published: April 2023

Mixed-species grassland containing legumes were suggested to increase yield compared to monocultures. Furthermore, some legumes were suggested to be able to sustain growth, even under drought conditions. The first aim of the current study was to measure if multispecies grassland with legumes is also more productive when their N input due to symbiotic N2 fixation is taken into account. Our second aim was to determine the benefit of grass–legume mixtures in terms of dry matter production under naturally occurring drought conditions. Mixed-species grasslands, consisting of monocultures and variable mixtures of (a) Trifolium pratense, (b) Trifolium. repens, (c) Lolium perenne, and (d) a mixture of drought-tolerant grasses (GSWT based), were assessed for their dry matter production over two years with contrasting weather patterns. The legume–grass seeding mixtures received either a fixed (180 kg N ha−1) or adapted N-fertilizer application (0–180 kg N ha−1), with the latter taking the assumed symbiotic N2 fixation by legumes into account. Mixed-species grassland showed improved yield compared to monocultures both in comparably humid and drought-affected years. The benefits of multispecies grass–legume mixtures were considerably more obvious under a fixed but still measurable under an adapted N-fertilizer regime. The species diversity effect appears to be significantly dependent on the additional N supply enabled by legumes’ symbiotic N2-fixation. Legumes and drought-tolerant grasses yielded equally well under drought conditions, although legumes showed major advantages during moderate drought and humid conditions. White and red clover, although both legumes, differed significantly in their persistence under elevated-N and their dry matter production under low-N fertilizer application, but were equal in their tolerance towards drought.
A Reaction of N-Substituted Succinimides with Hydroxylamine as a Novel Approach to the Synthesis of Hydroxamic Acids
Authors: Tretyakov Bogdan A. , Gadomsky Svyatoslav Y. , Terentiev Alexei A.
Published: April 2023

We describe a novel two-step approach for the synthesis of compounds with a hydroxyl-amide group (hydroxamic acids), which are widely known for their biological activity (histone deacetylase inhibitors, matrix metalloproteinases inhibitors and others). The first stage is the synthesis of N-substituted succinimide via the reaction of aromatic amine or carboxylic acid hydrazide with succinic anhydride. The second step involves the imide ring opening reaction by hydroxylamine. For both stages, universal synthetic methods are developed to exclude additional purification procedures for the target compounds. Sixteen hydroxamic acids are synthesized using the developed approach. Most of the compounds are obtained for the first time.
L-Tyrosine Limits Mycobacterial Survival in Tuberculous Granuloma
Authors: Gao Yaxian , Li Jiaqing , Guo Xinya , Guan Liru , Wang Jie , Huang Xiaochen , Wang Wenjuan , Yang Hua
Published: April 2023

Caused by the intracellular pathogen Mycobacterium tuberculosis (Mtb), tuberculosis (TB) remains a massive global public health issue. A well-known and key TB trait is caseous necrotic granuloma, which allows mycobacteria to reactivate and disseminate, thus confounding TB eradication programs. Amino acid (AA) metabolism is key to regulating immune responses in Mtb infections; however, it is currently unclear if AAs can be used to treat tuberculous granulomas. Here, we screened 20 proteinogenic AAs using a Mycobacterium marinum-infected zebrafish granuloma model. Only L-tyrosine simultaneously reduced Mycobacterium marinum (M. marinum) levels in zebrafish larvae and adults and inhibited intracellular pathogen survival levels. Mechanistically, L-tyrosine significantly upregulated interferon-γ (IFN-γ) expression in M. marinum -infected zebrafish adults but not in larvae. Using N-acetylcysteine (NAC) to inhibit reactive oxygen species (ROS), L-tyrosine appeared to inhibit Mtb intracellular survival by promoting ROS production. Thus, L-tyrosine as a non-essential AA may reduce mycobacterial survival in both macrophages and tuberculous granulomas. Our research provides a platform for the clinical development of AAs for active or latent TB patients infected with drug-sensitive or drug-resistant Mtb.
Implementation of a Patient Questionnaire in Community Pharmacies to Improve Care for Patients Using Combined Antithrombotic Therapy: A Qualitative Study
Authors: van Uden Renate C. A. E. , Bakker Marit A. , Joosten Stephan G. L. , Meijer Karina , van den Bemt Patricia M. L. A. , Becker Matthijs L. , Vervloet Marcia
Published: April 2023

For several indications or combinations of indications the use of more than one antithrombotic agent is required. The duration of combined antithrombotic therapy depends on indication and patient characteristics. This study investigated the use of an antithrombotic questionnaire tool that had been developed for pharmacists to detect patients with possible incorrect combined antithrombotic therapy. The objective of this study was to identify potential barriers and facilitators that could influence the implementation of the developed antithrombotic questionnaire tool in daily community pharmacy practice. A qualitative study was conducted at 10 Dutch community pharmacies in which the antithrombotic questionnaire tool had been used with 82 patients. Semi-structured interviews were conducted with pharmacy staff who used the antithrombotic questionnaire tool. The interview questions to identify barriers and facilitators were based on the Consolidated Framework for Implementation Research. The interview data were analysed using a deductive thematic analysis. Ten staff members from nine different pharmacies were interviewed. Facilitators for implementation were that the questionnaire was easily adaptable and easy to use, as well as the relative short duration to administer the questionnaire. A possible barrier for using the questionnaire was a lower priority for using the questionnaire at moments when the workload was high. The pharmacists estimated that the questionnaire could be used for 70–80% of the patient population and they thought that it was a useful addition to regular medication surveillance. The antithrombotic questionnaire tool can be easily implemented in pharmacy practice. To implement the tool, the focus should be on integrating its use into daily activities. Pharmacists can use this tool in addition to regular medication surveillance to improve medication safety in patients who use combined antithrombotic therapy.
Characterization of Purple Carrot Germplasm for Antioxidant Capacity and Root Concentration of Anthocyanins, Phenolics, and Carotenoids
Authors: Pérez María Belén , Carvajal Sofía , Beretta Vanesa , Bannoud Florencia , Fangio María Florencia , Berli Federico , Fontana Ariel , Salomón María Victoria , Gonzalez Roxana , Valerga Lucia , Altamirano Jorgelina C. , Yildiz Mehtap , Iorizzo Massimo , Simon Philipp W. , Cavagnaro Pablo F.
Published: April 2023

The present study characterized a genetically and phenotypically diverse collection of 27 purple and two non-purple (one orange and one yellow) carrot accessions for concentration of root anthocyanins, phenolics, and carotenoids, and antioxidant capacity estimated by four different methods (ORAC, DPPH, ABTS, FRAP), in a partially replicated experimental design comprising data from two growing seasons (2018 and 2019). Broad and significant (p < 0.0001) variation was found among the accessions for all the traits. Acylated anthocyanins (AA) predominated over non-acylated anthocyanins (NAA) in all the accessions and years analyzed, with AA accounting for 55.5–100% of the total anthocyanin content (TAC). Anthocyanins acylated with ferulic acid and coumaric acid were the most abundant carrot anthocyanins. In general, black or solid purple carrots had the greatest TAC and total phenolic content (TPC), and the strongest antioxidant capacities, measured by all methods. Antioxidant capacity, estimated by all methods, was significantly, positively, and moderately-to-strongly correlated with the content of all individual anthocyanins pigments, TAC, and TPC, in both years (r = 0.59–0.90, p < 0.0001), but not with the carotenoid pigments lutein and β-carotene; suggesting that anthocyanins and other phenolics, but not carotenoids, are major contributors of the antioxidant capacity in purple carrots. We identified accessions with high concentration of chemically stable AA, with potential value for the production of food dyes, and accessions with relatively high content of bioavailable NAA that can be selected for increased nutraceutical value (e.g., for fresh consumption).
Plant Use in the Late Renaissance Gardens of the 17–18th Century Transylvania
Authors: Fekete Albert , Sárospataki Máté
Published: April 2023

The aim of this article is to find, scientifically define, and locate the most frequent occurrences of the gardens of Transylvania in the Late Renaissance period (17–18th centuries), and to collect and prepare a comprehensive plant list of these gardens. During our investigation, based on archival and literary sources, as well as field studies carried out, we identified 81 Late Renaissance residency gardens located in Transylvania. We defined the most typical garden types for the region and we delineated the most characteristic ornamental, fruit, and vegetable plants, including fodder plants, used at that time in residential gardens. Meanwhile, the article intends to give a general overview of the first decisive time period in the Carpathian Basin, represented by the Late Renaissance garden art, from a garden and landscape architectural point of view.
Decision Making in Plants: A Rooted Perspective
Authors: Lee Jonny , Segundo-Ortin Miguel , Calvo Paco
Published: April 2023

This article discusses the possibility of plant decision making. We contend that recent work on bacteria provides a pertinent perspective for thinking about whether plants make choices. Specifically, the analogy between certain patterns of plant behaviour and apparent decision making in bacteria provides principled grounds for attributing decision making to the former. Though decision making is our focus, the discussion has implications for the wider issue of whether and why plants (and non-neural organisms more generally) are appropriate targets for cognitive abilities. Moreover, decision making is especially relevant to the issue of plant intelligence as it is commonly taken to be characteristic of cognition.
Effects of Spraying KH2PO4 on Flag Leaf Physiological Characteristics and Grain Yield and Quality under Heat Stress during the Filling Period in Winter Wheat
Authors: Li Jinpeng , Li Zhongwei , Li Xinyue , Tang Xiuqiao , Liu Huilian , Li Jincai , Song Youhong
Published: April 2023

As one of the most important wheat-producing areas in China, wheat is prone to heat stress during the grain filling period in the Huang-Huai-Hai Plain (3HP), which lowers yields and degrades the grain quality of wheat. To assess the effects of spraying potassium dihydrogen phosphate (KH2PO4) on the physiological traits in flag leaves and grain yield (GY) and quality under heat stress during the filling period, we conducted a two-year field experiment in the winter wheat growing seasons of 2020–2022. In this study, spraying water combined with heat stress (HT), 0.3% KH2PO4 (KDP), and 0.3% KH2PO4 combined with heat stress (PHT) were designed, and spraying water alone was used as a control (CK). The dates for the spraying were the third and eleventh day after anthesis, and a plastic film shed was used to impose heat stress on the wheat plants during the grain filling period. The results showed that spraying KH2PO4 significantly improved the chlorophyll content and net photosynthesis rate (Pn) in flag leaves compared with the non-sprayed treatments. Compared with CK, the Pn in HT decreased by 8.97% after heat stress, while Pn in PHT decreased by 7.44% compared to that of KDP. The activities of superoxide dismutase, catalase, and peroxidase in flag leaves were significantly reduced when the wheat was subjected to heat stress, while malonaldehyde content increased, and the enzyme activities were significantly enhanced when KH2PO4 was sprayed. Heat stress significantly decreased the contribution rate of dry matter accumulation (DM) after anthesis of wheat to grain (CRAA), whereas spraying KH2PO4 significantly increased the CRAA and harvest index. At maturity, the DM in CK was significantly higher than that in HT, KDP was significantly higher than PHT, and KDP had the highest DM. Compared with CK, the GY in KDP significantly increased by 9.85% over the two years, while the GY in HT decreased by 11.44% compared with that of CK, and the GY in PHT decreased by 6.31% compared to that of KDP. Spraying KH2PO4 after anthesis primarily helped GY by maintaining a high thousand grain weight to lessen the negative effects of heat stress on wheat. Moreover, heat stress significantly reduced protein concentration, wet gluten content, dough development time, and hardness index in grains of mature, while spraying KH2PO4 maintained a sufficient grain quality under the conditions of achieving higher yields. Overall, spraying KH2PO4 after anthesis could enhance the heat stress resistance of wheat and maintain the photosynthetic capacity of flag leaves, ensuring the dry matter production and reducing the negative effects on grain yield and quality in the 3HP.
Shrub Cover and Soil Moisture Affect Taxus baccata L. Regeneration at Its Southern Range
Authors: Calvia Giacomo , Casula Paolo , Farris Emmanuele , Fenu Giuseppe , Fantini Sergio , Bacchetta Gianluigi
Published: April 2023

The effect of key ecological and anthropic factors on the recruitment of the common yew (Taxus baccata L.) in Sardinia (Italy) has been analyzed. After bibliographic and cartographic research, followed by field surveys, we found 232 sites where yew grows in Sardinia (as opposed to 69 previously reported in the literature). Among them, we selected 40 sites, located in 14 different mountain chains, characterized by a number of individuals ranging from 11 to 836 adult yews with an average diameter at breast height (DBH) from 13 to 130 cm. By means of generalized linear modeling, we investigated and weighted the effect of ecological, structural, and anthropic factors on the amount of T. baccata recruitment. Our analyses showed that stand recruitment was positively correlated to shrub cover and soil moisture. In particular, shrub cover had a stronger effect, clearly showing that a thicker shrub layer, both bushy and/or spiny, corresponded to a higher number of yew seedlings and saplings. Secondarily, moister sites had a higher number of seedlings and saplings, showing that habitat suitability improved with higher humidity. On the contrary, recruitment was negatively correlated to browsing (both from livestock and wild animals). Our data confirm that the presence of a protective layer of shrubs is a crucial factor for seedling and sapling survival, mostly in relation to protection from summer drought and the browsing of large herbivores. Finally, guidelines for the conservation and restoration of T. baccata communities, referred to as the EU priority habitat 9580* (Mediterranean Taxus baccata woods), have been outlined.
Water Cannot Activate Traps of the Carnivorous Sundew Plant Drosera capensis: On the Trail of Darwin’s 150-Years-Old Mystery
Authors: Pavlovič Andrej , Vrobel Ondřej , Tarkowski Petr
Published: April 2023

In his famous book Insectivorous plants, Charles Darwin observed that the bending response of tentacles in the carnivorous sundew plant Drosera rotundifolia was not triggered by a drop of water, but rather the application of many dissolved chemicals or mechanical stimulation. In this study, we tried to reveal this 150-years-old mystery using methods not available in his time. We measured electrical signals, phytohormone tissue level, enzyme activities and an abundance of digestive enzyme aspartic protease droserasin in response to different stimuli (water drop, ammonia, mechanostimulation, chitin, insect prey) in Cape sundew (Drosera capensis). Drops of water induced the lowest number of action potentials (APs) in the tentacle head, and accumulation of jasmonates in the trap was not significantly different from control plants. On the other hand, all other stimuli significantly increased jasmonate accumulation; the highest was found after the application of insect prey. Drops of water also did not induce proteolytic activity and an abundance of aspartic protease droserasin in contrast to other stimuli. We found that the tentacles of sundew plants are not responsive to water drops due to an inactive jasmonic acid signalling pathway, important for the induction of significant digestive enzyme activities.