bluuebunny/crossref_metadata_2025 huggingface dataset:
Action Recognition and Application of Table Tennis Training Based on IOT Perception
Authors: Zhang Zhijian
Published: September 2022

As the national sport of our country, table tennis focuses on continuous innovation and development. Table tennis is a high-quality sport characterized by fast movement and great flexibility. Unlike other human behaviors, identifying ping pong shots is idiosyncratic and difficult. Table tennis training is extremely important, and correct training can make athletes progress. In this paper, based on the perception of the Internet of Things, the action recognition and application of table tennis training actions are carried out, and the following conclusions are drawn: (1) the training movements of table tennis are relatively complex, which has a great challenge to the action recognition technology. The action recognition technology based on IoT perception can efficiently identify table tennis training actions. (2) Analyze and study the action recognition algorithm based on IoT perception, and propose a higher recognition accuracy and more stable algorithm DTW. (3) Comparing the accuracy, loss rate, and time impact between the algorithm in this paper and the traditional recognition algorithm, it is concluded that the algorithm proposed in this paper has higher accuracy and lower loss rate than the traditional algorithm. And with good stability, it is not affected by the environment and time. The algorithm in this paper is an algorithm with better performance and more worthy of use.
[Retracted] Exploration of Happy Learning for College Students with the Integration of Thinking Visualization and Action Learning in the Internet + Environment
Authors: Han Zhen
Published: September 2022

Under the background of networked education, the subjective behavior in the field of education, the interaction between teachers and students, and the educational environment in which the subject is located have all had a comprehensive impact on the teaching mode of mental health courses in colleges and universities. In recent years, the teaching research and practice related to “action learning” and “thinking visualization” have been deepening, and more papers, monographs, and training activities related to them have been deepened. Thinking is a complex cognitive process, and students’ positive and critical thinking attitude plays a crucial role in improving the quality of nursing work in the future. The importance of study, our way of self‐cultivation, and the foundation of our destiny is beyond words. This study explores the application and significance of college students’ happy learning combined with the visualization of thinking and action learning under the background of “Internet +” from four aspects: curriculum connotation, system construction, effect analysis, and curriculum thinking, so that students can play their subjectivity in education courses and improve. The initiative and interaction of students in the classroom encourages students to not only know, feel, and experience in education courses but also dare to speak, ask, and question and be the leaders of the classroom.
[Retracted] Risk Management of Sports Venues and Olympic Sports Cooperation Spirit under Complex Environment
Authors: Jiang Dianzai
Published: September 2022

The understanding of sports event risk and Olympic sports cooperation spirit, as well as the systematic analysis and research of sports event risk management, are still in their early stages in China’s sports theory circle. Institutions for developing sports talent also disregard programmes designed to increase the risk awareness and Olympic spirit of sports workers. This essay provides an overview of the concepts of sports event risk and Olympic sports cooperation spirit based on reality. This paper examines the interaction of risk management of large‐scale sporting events and Olympic sports cooperation spirit from the perspective of event organization managers, using the pertinent theory of risk management and the random forest algorithm in machine learning. The experimental results show that, according to the analysis of injury occurrence according to different positions, it is discovered that forward athletes suffer the most injuries, with an average of 54 forwards in injury situations, followed by guards with an average of 43 guards in injury situations, and finally centres with an average of 38 guards in injury situations. Combining risk management for major sporting events with the cooperative Olympic sports spirit has significant practical implications.
CCCAP-Pre: Predicting Price of Artwork Based on GM(1, N, x(1)) Model and Cultural Services
Authors: Tang Jiajia , Cheng Yue , Shao Yu-Tao , Xiao Xuan
Published: September 2022

Predicting price of contemporary ceramic artworks is an important and difficult problem, particularly when every object is unique and potential bidder’s tastes may exhibit substantial variation. In recent years, China’s ceramic art market has shown a considerable developing trend, but at the same time, there are also problems that severely restrict its development, such as the chaotic price system. As cultural products, contemporary ceramic artworks have the value of cultural services. Unfortunately, the existing price evaluation models all ignore cultural services. By introducing the “cultural services” and “gray model GM(1, N, x(1)),” a new predictor, called CCCAP-Pre, has been developed to predict prices of contemporary ceramic artworks. As demonstrated, the minimum error, the maximum error, and the average relative error of CCCAP-Pre were 0.02%, 6.19%, and 1.40% on ceramic sculpture artworks and 0.06%, 9.11%, and 3.63% on ceramic painting artworks, respectively. It will provide a reference for the benign development of ceramic art market.
Construction of College Students’ Mental Health Assessment and Art Therapy System Aided by the Internet of Things and Big Data
Authors: Li Qing
Published: September 2022

In order to improve the effect of college students’ mental health assessment, this paper combines the Internet of Things and big data technology to build a college student’s mental health assessment system and analyzes and verifies the clustering effectiveness indicators by using FCM, GK, and GG clustering algorithms. Moreover, this paper explores the basic concepts and processes of FCM algorithm, GK algorithm, and GG algorithm and expounds the connection between the three algorithms. In addition, this paper uses four datasets to conduct clustering experiments and compares the CS indicator with several other indicators. The experimental results demonstrate the effectiveness of the CS indicator. The simulation study shows that the student mental health assessment system based on the Internet of Things and big data technology proposed in this paper can play a certain role in student mental health and art therapy, and it also verifies that art therapy plays a certain role in student psychotherapy.
[Retracted] Analysis of Erhu Performance Effect in Public Health Music Works Based on Artificial Intelligence Technology
Authors: Yang Li
Published: September 2022

With the rise of Erhu teaching in recent years, a large number of people have joined the team to learn Erhu playing. However, due to the high cost of teaching and the unique one‐to‐one teaching mode between teachers and students, Erhu education resources are very scarce. Learning Erhu performance has become a luxury activity. Nowadays, with the rise of artificial intelligence, computer music is developing rapidly. Music has two important aspects: composition and performance. Different kinds of instruments convey different styles, and players inject different rhythms and dynamics into their performance, thus producing rich expressive force. The development of image style conversion, which opens people’s evaluation of music performance, is an important issue in many fields of artificial intelligence (it is also known as intelligence, machine intelligence, referring to the intelligence shown by the machine made by people. Usually, artificial intelligence refers to the technique of presenting human intelligence through ordinary computer programs). For an Erhu song, there are various factors that affect its effectiveness, and there are many indexes to evaluate it, such as sense of rhythm, expressive force, musical sense, style, and so on. Using a computer to simulate the evaluation process is essential to find out the mathematical relationship between the factors that affect the performance of music and the evaluation indexes. Neural network is a kind of mathematical model proposed by simulating the way of thinking of human brain in artificial intelligence. It has the advantages of not having strict requirements on data distribution, nonlinear data processing method, strong robustness, and dynamics and is very suitable for the mathematical model of evaluation system. In addition, the neural network also has a strong theoretical basis, and their application in various industries has developed basically mature. This paper tries to introduce a deep neural network mathematical model into the evaluation system of Erhu performance, and the experimental results prove the reliability and practicality of the method in this paper. It can provide a method basis and theoretical reference for evaluation of Erhu performance effect.
Study on the Inerting Effect and Migration Law of Nitrogen and Carbon Dioxide in Large Inclined Goaf by Physical Simulation Model
Authors: Song Xiaolin , Wu Chunlei , Liu Chun , Li Yanlong , Wang Zhiyu , Shi Bobo
Published: September 2022

In this paper, the inerting effect and migration law of inert N2 and CO2 in large inclined goaf are studied using a physical simulation model. The differences in oxygen reduction and inert gas migration are analyzed and compared. The results show that as N2 and CO2 injection increased, the oxidation zone’s range and width shrunk, and the end of the loose and oxidation zones moved closer to the working face. The migration profile of CO2 and N2 resembled a trumpet and an L shape, respectively, and under the same inerting flow rate, the effect of CO2 inerting on oxygen reduction was much less significant than that of N2.
Abstract IA01: Molecular drivers in hepatocellular carcinoma: The story of what we know and what are we missing
Authors: Zucman-Rossi Jessica
Published: September 2022

Hepatocellular carcinoma (HCC) is a highly heterogeneous cancer, both at the molecular and histological level. High-throughput sequencing and gene expression profiling have identified distinct transcriptomic subclasses and recurrently altered cancer driver genes. Several HCC subtypes characterized by histological features have also been identified. HCC phenotype appears to be closely related to particular gene mutation and driven by various oncogenic pathway alterations. Identification of the origin of HCC development and their trajectory of progression is important to understand tumor evolution and to identify the mechanisms of tumor response or resistance to therapies. We will review the early and late molecular alterations driving HCC occurrence and progression of tumors in the context of patient lifespan and exposure to environmental factors. Several questions remain to be answered, in particular on the identification of the cells at the origin of the malignant transformation into HCC and the mechanism of escape to the immunological monitoring.

Citation Format: Jessica Zucman-Rossi. Molecular drivers in hepatocellular carcinoma: The story of what we know and what are we missing [abstract]. In: Proceedings of the AACR Special Conference: Advances in the Pathogenesis and Molecular Therapies of Liver Cancer; 2022 May 5-8; Boston, MA. Philadelphia (PA): AACR; Clin Cancer Res 2022;28(17_Suppl):Abstract nr IA01.
Abstract IA03: Polyploidy, DNA damage response driver or gatekeeper of chronic liver diseases
Authors: Desdouets Chantal
Published: September 2022

Polyploidy, also known as whole-genome amplification, is a condition in which the organism has more than the two basic sets of chromosomes. Polyploidy frequently arises during tissue development and repair, and in age-associated diseases, such as cancer. Its consequences are diverse and clearly different between systems. The liver is a particularly fascinating organ in that it can adapt its ploidy to the physiological and pathological context. In human liver parenchyma, polyploidy is mainly cellular (binuclear hepatocytes) with no specific zonation across the liver lobule. During liver tumorigenesis, cellular ploidy is drastically reduced; binuclear polyploid hepatocytes are barely present in HCC tumors. Remarkably, nuclear ploidy is specifically amplified in HCC tumors. In fact, nuclear ploidy is amplified in HCCs harboring a low degree of differentiation and TP53 mutations. Highly polyploid tumors are associated with a poor prognosis. Hepatocytes increase their ploidy also during the proliferative stress that underlies various liver disease conditions. Recently, we investigated mechanisms underlying activation of the DNA damage response during Non-alcoholic Steatotic Liver Disease. We demonstrated that proliferating mouse NAFLD hepatocytes harbor replication stress with an alteration of the replication fork’s speed and activation of ATR pathway which is sufficient to cause DNA breaks. Nucleotide pool imbalance occurring during NAFLD is the key driver of replication stress. Remarkably, DNA lesions drive cGAS/STING pathway activation, a major component of cells’ intrinsic immune response. The translational significance of this study was reiterated by showing that lipid overload in proliferating HepaRG was sufficient to induce replication stress and nucleotide pool imbalance. Moreover, livers from NAFLD human patients displayed nucleotide pathways deregulation and cGAS/STING genes alteration. Altogether, our findings shed new light on the mechanisms by which damaged NAFLD hepatocytes might promote disease progression.

Citation Format: Chantal Desdouets. Polyploidy, DNA damage response driver or gatekeeper of chronic liver diseases [abstract]. In: Proceedings of the AACR Special Conference: Advances in the Pathogenesis and Molecular Therapies of Liver Cancer; 2022 May 5-8; Boston, MA. Philadelphia (PA): AACR; Clin Cancer Res 2022;28(17_Suppl):Abstract nr IA03.
Abstract IA11: Molecular targeted therapies for advanced HCC
Authors: Llovet Josep M.
Published: September 2022

Hepatocellular carcinoma (HCC) remains one of the most prevalent and deadliest cancers. Current major advancements have impacted the management of patients with advanced HCC substantially improving their life expectancy. Several systemic therapies have resulted in positive III investigations (atezolizumab plus bevacizumab, durvalumab plus tremelimumab, sorafenib, lenvatinib, regorafenib, cabozantinib and ramucirumab) and two additional therapies obtained accelerated FDA approval owing to evidence of efficacy (pembrolizumab and nivolumab plus ipilimumab). Specifically, among TKIs, sorafenib and lenvatininb remain as first line option therapies in patients with contraindications or upon progression to immunotherapies. In second-line, regorafenib, cabozantinib and ramucirumab are the accepted choices. Few studies suggest that biomarkers or treatment related-AEs are associated with better outcome, as it is the case with hand-foot skin reaction with sorafenib, or AFP> 400 ng/ml or hypertension for ramucirumab. The fact that most HCC do not have a dominant actionable target has prevented precision oncology. Nonetheless, current studies are exploring abrogating main drivers previously unactionable, such as wnt signaling. The fact that TKI and VEGF-based MAb have also immunomodulatory activities has boosted their combination with immune therapies which nowadays are dominant in the landscape of management of HCC and ongoing phase III trials.

Citation Format: Josep M. Llovet. Molecular targeted therapies for advanced HCC [abstract]. In: Proceedings of the AACR Special Conference: Advances in the Pathogenesis and Molecular Therapies of Liver Cancer; 2022 May 5-8; Boston, MA. Philadelphia (PA): AACR; Clin Cancer Res 2022;28(17_Suppl):Abstract nr IA11.
Abstract IA20: Combination therapies including checkpoint inhibitors in HCC: the dawn of a new era
Authors: Finn Richard S.
Published: September 2022

After a decade of stalled progress in the medical management of advanced HCC we have seen rapid progress in improving survival for patients with advanced HCC. We have gone from incremental improvements with sequencing multi-kinase inhibitors to significant gains with immunotherapy-based combinations. Arguably, the success of combinations is driven by the increased response rates seen with this approach as compared to the challenges seen with single agent checkpoint inhibitors. Until biomarkers of response are identified, the continued development of new combinations will still be driven an all-comers approach, with the empiric addition of new agents to already established doublets. These new combinations will likely benefit from lessons learned in other diseases where immune checkpoint inhibitors have been used for much longer periods of time (melanoma, lung cancer). The challenge though, is when we look at progress in these other diseases, the next combination to move things forward is yet to be determined, whether in the front-line setting, or after progression on IO. Ongoing translational studies are offering insights into the mechanisms by which we are seeing increased activity with combinations, but the clinical utility of these data have not yet been determined. In the lecture we will review the data as it stands today and offer thoughts on the next generation of studies with systemic therapy in HCC.

Citation Format: Richard S. Finn. Combination therapies including checkpoint inhibitors in HCC: the dawn of a new era [abstract]. In: Proceedings of the AACR Special Conference: Advances in the Pathogenesis and Molecular Therapies of Liver Cancer; 2022 May 5-8; Boston, MA. Philadelphia (PA): AACR; Clin Cancer Res 2022;28(17_Suppl):Abstract nr IA20.
Abstract IA23: Molecular landscapes of hepatocellular carcinoma and cholangiocarcinoma
Authors: Wang Xin Wei
Published: September 2022

Liver cancer is among the top five deadliest cancers in the world, especially in Southeast Asia and Sub-Saharan Africa, and its incidence rates continue to increase in recent decades. Despite considerable efforts towards the development of new prevention, diagnosis and treatment strategies, the improvement of cancer survival is modest. Defined as the unique genotypic and phenotypic differences of cancer cells within a single tumor (intra-tumor) or amongst different patients (inter-tumor), tumor heterogeneity has consistently been linked to worse clinical outcomes in liver cancer. Primary liver cancer mainly consists of two clinical types, i.e., hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), each of which could be further divided into several clinical and molecular subtypes. Chronic liver diseases due to viral hepatitis, alcohol consumption, chemical carcinogens, or nonalcoholic fatty liver disease/nonalcoholic steatohepatitis, are major global health burdens that increase the risk of HCC while parasitic infections may be linked to CCA. It is unclear how complex etiological factors determine tumor subtypes and whether common features may be shared among HCC and CCA. Given the presence of so many clinical and molecular variables, it is imperative to develop well-defined cohorts that include diverse etiologies, race/ethnicities, sex and age, which provides an unbiased platform by minimizing confounding factors to study liver cancer types. Accordingly, we have established several national and international collaborative projects, including the NCI-CLARITY study, the NCI-UMD cohort study, the NCI-Mongolian cohort study, and the TIGER-LC (Thailand) consortium. We have applied molecular-based technologies such as genomics, transcriptomics, metabolomics and microbiomics, including single-cell omics to comprehensively analyze biopsies from diverse populations, in order to better characterize heterogeneity among and within patients, to further define tumor molecular subtypes with unique tumor biology and to understand tumor evolution in response to treatment. These studies have led to the discovery of new cell types and drivers in HCC and CCA, potential new mechanisms of therapy resistance and tumor progression, and new insights into the evolutionary patterns of liver cancer. Our past and future ability to translate our research findings towards patient management through the identification of molecular-based knowledge for understanding of liver cancer pathophysiology with the application of early detection and treatment of liver cancer may have a considerable impact on clinical practice and public health.

Citation Format: Xin Wei Wang. Molecular landscapes of hepatocellular carcinoma and cholangiocarcinoma [abstract]. In: Proceedings of the AACR Special Conference: Advances in the Pathogenesis and Molecular Therapies of Liver Cancer; 2022 May 5-8; Boston, MA. Philadelphia (PA): AACR; Clin Cancer Res 2022;28(17_Suppl):Abstract nr IA23.
Abstract IA27: Trial Design in the era of precision oncology and combination therapies
Authors: Bridgewater John
Published: September 2022

Clinical studies in precision medicine have tested the long-standing conventional model built around the randomized phase 3 study. Multiple uncertainties with respect to incidence, molecular profiling and realistic level of evidence have challenged tried and tested paradigms for trialists, clinicians and reimbursement authorities. I will discuss how we determine the incidence of uncommon subgroups of uncommon cancers, the definition of these subgroups using bespoke clinical and molecular criteria, the availability, efficacy and heterogeneity of molecular testing, the availability of specialists, the quality of the data in order to permit reimbursement and the sustainability of treating patients in the era of precision medicine. With the continuing evolution of precision medicine, old dogs will have to become familiar with many new tricks.

Citation Format: John Bridgewater. Trial Design in the era of precision oncology and combination therapies [abstract]. In: Proceedings of the AACR Special Conference: Advances in the Pathogenesis and Molecular Therapies of Liver Cancer; 2022 May 5-8; Boston, MA. Philadelphia (PA): AACR; Clin Cancer Res 2022;28(17_Suppl):Abstract nr IA27.
Abstract PO002: Pretreatment immune cell composition and checkpoint ligand expression define the response to immunotherapy in advanced HCC: a study using single-cell sequencing
Authors: Cappuyns Sarah , Philips Gino , Vandecaveye Vincent , Verslype Chris , Van Cutsem Eric , Lambrechts Diether , Derkervel Jeroen
Published: September 2022

The novel combination of atezolizumab/bevacizumab has demonstrated unprecedented response rates in a subgroup of advanced hepatocellular carcinoma (aHCC) patients. In order to understand why only a subset of aHCC patients respond to immunotherapy targeting the PD-1/PD-L1 axis, we subjected pre-treatment tissue biopsies from 31 aHCC patients, with mainly non-viral etiologies, to simultaneous single-cell transcriptome (scRNAseq) and T-cell receptor (scTCRseq) sequencing, providing an integrative view of the tumor-microenvironment of aHCC (n=91 169 cells). The therapeutic targets, PD-1 and PD-L1 are expressed in tumor-infiltrating T-cells versus myeloid cells and tumor cells, respectively. Myeloid cells in tumors responding to immunotherapy (according to mRECIST) express significantly higher levels of PD-L1 (p=0.014) compared to non-responders and this is driven by pro-inflammatory PD-L1 expressing CXCL10+ macrophages, involved in T-cell recruitment. In contrast, non-responding tumors are infiltrated by immature CX3CR1+ macrophages and CCR2+ ‘monocyte-like’ macrophages (p=0.009 and p=0.01, respectively). Interestingly, the composition of tumor-infiltrating T-cells did not differ significantly between patient groups. However, responding tumors were characterized by a more clonal baseline T-cell receptor repertoire (p=0.007). Single-cell T-cell receptor sequencing revealed that this clonality was present in activated, effector T-cells, while non-responders had a higher proportion of non-clonal T-cells (p=0.01) concentrated within naïve and memory-like T-cell phenotypes. In conclusion, this is the first and largest aHCC cohort to correlate single-cell sequencing data with clinical outcomes. Prior to treatment, differences in abundancy and ligand expression in small clusters of immune cells as well as difference in baseline T-cell clonality drive response to checkpoint inhibition in aHCC.

Citation Format: Sarah Cappuyns, Gino Philips, Vincent Vandecaveye, Chris Verslype, Eric Van Cutsem, Diether Lambrechts, Jeroen Derkervel. Pretreatment immune cell composition and checkpoint ligand expression define the response to immunotherapy in advanced HCC: a study using single-cell sequencing [abstract]. In: Proceedings of the AACR Special Conference: Advances in the Pathogenesis and Molecular Therapies of Liver Cancer; 2022 May 5-8; Boston, MA. Philadelphia (PA): AACR; Clin Cancer Res 2022;28(17_Suppl):Abstract nr PO002.
Abstract PO005: Obesity-associated Visfatin, and Silibinin in an In Vitro and In Vivo Model of Liver Cancer
Authors: Pedone Elisa , Green Kelly N , Thornton Kelly R , Price Ramona S.
Published: September 2022

Introduction: Obesity, specifically body fatness, is a risk factor for the development of liver cancer. The link between obesity and liver cancer is likely to be mediated in part by a state of chronic inflammation. However, it has yet to be determined if visfatin inhibition, a pro-inflammatory adipocytokine upregulated by obesity, is sufficient to prevent or delay the progression of liver cancer. Obesity-induced inflammatory factors may promote cancer progression by increasing cell proliferation, motility, invasion, and metastasis.The use of dietary compounds may break the pro-inflammatory cycle induced by the obese state, thus attenuating the cancer process. Silibinin, a polyphenol in milk thistle seed, has been shown to have anti-inflammatory properties. Studies have yet to determine whether silibinin can be used to break the obesity-cancer link to delay progression of liver cancer. The objective of this cell culture and mouse study is to determine the efficacy of silibinin in the prevention of obesity-associated liver cancer progression. Methods: SNU-449 and HepG2 liver cancer cells were exposed to the following experimental conditions; control, FBS, visfatin (80 ng/ml), visfatin + silibinin 40 μM, silibinin (40 μM). Cell proliferation, lipogenesis, lactate dehydrogenase secretion, and ROS production were measured. Immunoblot was used to measure phospho-Akt, phospho-Erk, NF-kB, Bcl-xL, and actin. Eight-week-old C57BL/6J male mice were randomized to a high-fat DIO (n=6) or control diet (n=6). At 14 weeks, mice will be injected subcutaneously with syngeneic Hepa1-6 murine liver cancer cells. At 15-weeks, mice will receive peritoneal injections of silibinin 3 times per week for 2 weeks. Tumors will be measured and assessed for visfatin, Akt, Erk, and NF-kB protein expression. Results from the animal study are on-going and will be presented at time of the conference. Results: SNU-449 and HepG2 cells exposed to visfatin significantly increased cell proliferation and lipogenesis when compared to control. Further, the addition of silibinin inhibited the visfatin-induced proliferation and lipogenesis in both cell lines. Visfatin increased the phosphorylation of Akt and Erk, and NF-kB expression in both liver cancer cell lines. The addition of silibinin differently suppressed phosphorylation of Akt and Erk in SNU-449 and HepG2 cells. Conclusion: Silibinin differentially inhibits visfatin-induced cell growth, lipogenesis, and promotes cytotoxicity which may lead to increased cancer cell death.

Citation Format: Elisa Pedone, Kelly N Green, Kelly R Thornton, Ramona S. Price. Obesity-associated Visfatin, and Silibinin in an In Vitro and In Vivo Model of Liver Cancer [abstract]. In: Proceedings of the AACR Special Conference: Advances in the Pathogenesis and Molecular Therapies of Liver Cancer; 2022 May 5-8; Boston, MA. Philadelphia (PA): AACR; Clin Cancer Res 2022;28(17_Suppl):Abstract nr PO005.
Abstract PO017: HBV alters YAP regulation in liver cancer by remodeling PP2A complexes
Authors: Gordan John , Pitea Adriana , Turnham Rigney E , Eckhardt Manon , Jang Gwendolyn M , Lim Huat , Choi Alex L , Bandyopadhyay Sourav , Swaney Danielle , Shokat Kevan , Ideker Trey , Krogan Nevan
Published: September 2022

Viral hepatitis promotes hepatocellular carcinoma (HCC) initiation by inducing inflammation, cellular stress and cell death. However, the cell-intrinsic effects of viral infection in advanced HCC remain unclear. We used affinity purification mass spectrometry to develop a complete map of 145 interactions among Hepatitis B Virus (HBV) and host proteins in the HUH7 HCC tumor cell line, identifying known and novel HBV/host protein-protein interactions. We integrated this map with HCC genomes, identifying 61 proteins with preferential mutation in non-HBV HCC, suggesting that their interaction with HBV plays a role in cancer. Focusing on proteins that directly interact with the HBV oncoprotein X (HBx), we found that HBx rewires the effect of the PP2A phosphatase on HCC signaling. HBx binding excluded striatin-family regulatory subunits from the PP2A complex, causing Hippo kinase activation. This effect creates a requirement for integrin signaling to mTOR complex 2 to maintain expression of the YAP oncoprotein, critical for HCC growth. Thus, HBV rewires HCC signaling and may promote targetable dependencies.

Citation Format: John Gordan, Adriana Pitea, Rigney E Turnham, Manon Eckhardt, Gwendolyn M Jang, Huat Lim, Alex L Choi, Sourav Bandyopadhyay, Danielle Swaney, Kevan Shokat, Trey Ideker, Nevan Krogan. HBV alters YAP regulation in liver cancer by remodeling PP2A complexes [abstract]. In: Proceedings of the AACR Special Conference: Advances in the Pathogenesis and Molecular Therapies of Liver Cancer; 2022 May 5-8; Boston, MA. Philadelphia (PA): AACR; Clin Cancer Res 2022;28(17_Suppl):Abstract nr PO017.
Abstract PR05: A novel kinase inhibitor for B-catenin mutant hepatocellular carcinoma
Authors: Rialdi Alexander , Duffy Mary , Scopton Alex , Lujambio Amaia , Dar Arvin , Guccione Ernesto
Published: September 2022

Purpose: We recently developed a chemical strategy for the diversification and disease-specific improvement of tool and FDA-approved kinase inhibitors, and sought to use this strategy for genotype-specific drug discovery in hepatocellular carcinoma (HCC). Experimental Procedures: Primary and murine-derived HCC organoids were used for drug screening. Expansive multiomic (phosphoproteomics, RNAseq, and ChIPseq) and target engagement analyses were used in combination with in vitro rescue and in vivo efficacy experiments. Data Summary: Currently there are few personalized therapeutic approaches for advanced HCC, which is a leading cause of cancer-related deaths worldwide. b-catenin (CTNNB1)-mutated HCC represents 30% of the disease and is a subset of Wnt-driven tumors particularly unresponsive to current therapies. Using focused chemical libraries derived from clinical multi-kinase inhibitor scaffolds, we screened HCC organoids to identify a CTNNB1-mutant selective small molecule that we dub WNTinib. WNTinib retained exquisite selectivity in human and murine models expressing oncogenic b-catenin, including patient samples. Our multidisciplinary approach revealed that WNTinib inhibits KIT/MAPK signaling at multiple nodes. Moreover, we demonstrate that lack of direct engagement on BRAF and p38a/b kinases differentiates WNTinib from existing clinical kinase inhibitors, is necessary to avoid compensatory feedback signaling, and thereby provides a durable and selective transcriptional block of mutant b-catenin and Wnt targets through re-activation of cytoplasmic enhancer of zest homolog 2 (EZH2). Finally, we demonstrate that the unique mechanism of WNTinib provides superior activity compared to clinical therapies in vivo, including in drug-resistant models of mutant b-catenin HCC. Conclusion: Our studies identify a novel inhibitor of the Wnt pathway and uncover a previously unknown mechanism to harness the KIT/MAPK/EZH2 pathway to potently and selectively antagonize CTNNB1-mutant HCC with an unprecedented wide therapeutic index. Our study also provides the rationale to explore WNTinib in proof-of-concept trials with enriched CTNNB1- mutant advanced HCC patients.

Citation Format: Alexander Rialdi, Mary Duffy, Alex Scopton, Amaia Lujambio, Arvin Dar, Ernesto Guccione. A novel kinase inhibitor for B-catenin mutant hepatocellular carcinoma [abstract]. In: Proceedings of the AACR Special Conference: Advances in the Pathogenesis and Molecular Therapies of Liver Cancer; 2022 May 5-8; Boston, MA. Philadelphia (PA): AACR; Clin Cancer Res 2022;28(17_Suppl):Abstract nr PR05.
Sociocultural Factors, Access to Healthcare, and Lifestyle: Multifactorial Indicators in Association with Colorectal Cancer Risk
Authors: Warren Andersen Shaneda , Zheng Wei , Steinwandel Mark , Murff Harvey J. , Lipworth Loren , Blot William J.
Published: September 2022

Black Americans of low socioeconomic status (SES) have higher colorectal cancer incidence than other groups in the United States. However, much of the research that identifies colorectal cancer risk factors is conducted in cohorts of high SES and non-Hispanic White participants. Adult participants of the Southern Community Cohort Study (N = 75,182) were followed for a median of 12.25 years where 742 incident colorectal cancers were identified. The majority of the cohort are non-Hispanic White or Black and have low household income. Cox models were used to estimate HRs for colorectal cancer incidence associated with sociocultural factors, access to and use of healthcare, and healthy lifestyle scores to represent healthy eating, alcohol intake, smoking, and physical activity. The association between Black race and colorectal cancer was consistent and not diminished by accounting for SES, access to healthcare, or healthy lifestyle [HR = 1.34; 95% confidence interval (CI),1.10–1.63]. Colorectal cancer screening was a strong, risk reduction factor for colorectal cancer (HR = 0.65; 95% CI, 0.55–0.78), and among colorectal cancer-screened, Black race was not associated with risk. Participants with high school education were at lower colorectal cancer risk (HR = 0.81; 95% CI, 0.67–0.98). Income and neighborhood-level SES were not strongly associated with colorectal cancer risk. Whereas individual health behaviors were not associated with risk, participants that reported adhering to ≥3 health behaviors had a 19% (95% CI, 1–34) decreased colorectal cancer risk compared with participants that reported ≤1 behaviors. The association was consistent in fully-adjusted models, although HRs were no longer significant. Colorectal cancer screening, education, and a lifestyle that includes healthy behaviors lowers colorectal cancer risk. Racial disparities in colorectal cancer risk may be diminished by colorectal cancer screening.

Prevention Relevance:

Colorectal cancer risk may be reduced through screening, higher educational attainment and performing more health behaviors. Importantly, our data show that colorectal cancer screening is an important colorectal cancer prevention strategy to eliminate the racial disparity in colorectal cancer risk.

See related Spotlight, p. 561
Quantifying the Effect of Physical Activity on Endometrial Cancer Risk
Authors: Kitson Sarah J. , Aurangzeb Olivia , Parvaiz Jawaria , Lophatananon Artitaya , Muir Kenneth R. , Crosbie Emma J.
Published: September 2022

Endometrial cancer incidence is rising, with 435,000 global cases in 2019. An effective, low-cost primary prevention strategy is required to reduce disease burden. Obesity, insulin resistance, and inflammation contribute to endometrial carcinogenesis and physical activity targets these pathways. This study sought to quantify the amount of physical activity required to impact upon endometrial cancer risk. Physical activity data from 222,031 female participants with an intact uterus in the UK Biobank study were analyzed using a multivariable Cox proportional hazards model. A systematic review of the literature was performed, searching CENTRAL, Embase, and MEDLINE databases up to April 19, 2021. Studies including participants with and without endometrial cancer investigating the effect of physical activity measured in MET-hours/week (MET-h/week) on disease risk were included. Two reviewers independently selected studies, extracted data, and evaluated the risk of bias. Within the UK Biobank, each 1 MET-h/week increase in total physical activity was associated with a 0.2% [95% confidence interval (CI), 0.1–0.4; P = 0.020] reduction in endometrial cancer risk, equating to a 10.4% reduction if performing 50 MET-h/week or 7 hours of jogging per week. Eleven cohort and 12 case–control studies were identified in the systematic review, including 821,599 participants. One study reported a nonsignificant effect of 1 MET-h/week increases in physical activity on endometrial cancer risk (OR, 1.00; 95% CI, 0.99–1.00). Eight studies found significant reductions in disease risk of 15%–53%, but only in the most physically active individuals. Physical activity reduces endometrial cancer risk, but the effect size appears small. Regular vigorous activity should be encouraged to maximize the health benefit observed.

Prevention Relevance:

Effective, low-cost primary prevention strategies are urgently needed to tackle the rapid global increase in endometrial cancer. We sought to quantify the effect of physical activity on endometrial cancer risk, noting a linear inverse relationship influenced by body mass index. The most beneficial type and amount of activity remain unclear.
What Are the Factors Underlying Colorectal Cancer Health Disparities?
Authors: Petrick Jessica L. , Barber Lauren E. , Rosenberg Lynn
Published: September 2022

Black Americans have the highest colorectal cancer incidence and mortality rates of any U.S. racial/ethnic group. Warren Andersen and colleagues report that sociocultural, lifestyle, and healthcare factors did not explain the racial disparity in colorectal cancer incidence, but colorectal cancer screening lessened the disparity. While screening is a cornerstone of colorectal cancer prevention, an improved understanding of etiologic factors may inform additional strategies for primary prevention or risk stratification. As important “established” colorectal cancer risk factors have not been corroborated for Black Americans, this begs the question of what other etiologic factors are important for colorectal cancer development in Black American populations.

See related article, p. 595
Violencia simbólica en relaciones de pareja desde la marginalidad en mujeres jóvenes
Authors: Cadavid-Marín Angela María , Marínez-Garcés Janier Deyanira
Published: September 2022

(analítico)El método biográfico narrativo permitió escuchar a seis mujeres jóvenes que habitan contextos marginales. A partir de un diseño narrativo, se concretaron narrativas biográficas; en ellas expusieron los modos como han configurado sus relaciones de pareja en torno a las prácticas y discursos de la violencia simbólica, dejando al descubierto una realidad atravesada por la violencia de un sector enclavado en el olvido institucional y el señalamiento colectivo. El análisis de la información se realizó mediante un Biograma, el cual permitió encontrar que la despolitización y la idealización son los  modos como han construido sus relaciones. La relevancia de este estudio se centra en dar a conocer la resistencia a los mecanismos de poder como posibilidad de transformar la construcción de amor que habitualmente se ha promovido en otras mujeres..
Palabras clave:  Jóvenes; marginalidad; relaciones (de pareja); violencia (simbólica); despolitización; idealización.
A new species and record of Biancolina Della Valle, 1893 (Amphipoda, Senticaudata, Ampithoidae) from the Southwestern Atlantic
Authors: Andrade Renan de Melo , Souza-Filho Jesser Fidelis
Published: September 2022

Here we describe a new species of Biancolina from Pernambuco, Brazil, found in association with the brown algae Sargassum spp., between 0.5 and 1.0 m depth. The new species differs from its congeners mainly by the following combination of characters: antenna 1 twice the length of antenna 2, lacinia mobilis present in both mandibles, article 4 of the maxilliped bulbose; coxa 6 with anteroventral lobe well developed as in posteroventral lobe, peduncle of uropod 1 weakly setose, palm of pereopods 5-7 lacking robust seta. This is the first record of Biancolina from the southwestern Atlantic.
Recensional Traces in Codex Vaticanus in the Light of Papyrus 967
Authors: Candido Dionisio
Published: September 2022

This article focuses on some readings of the book of Esther in Codex Vaticanus (4th century CE), unanimously regarded as the best witness of the Old Greek text. A first comparison is made with the text of 𝔓 967 (3rd century CE), a special papyrus within the Greek textual tradition. All the relevant differences between the two are considered. Once scribal error is excluded, attention is directed to possible parallel readings with the Masoretic Text in order to ascertain whether these readings show traces of a revision of the text of Codex Vaticanus (B) towards MT.
Observations Concerning the Origins and the Relation of Codex Vaticanus to Codex Sinaiticus
Authors: Kreuzer Siegfried
Published: September 2022

This paper analyzes the origin of Codex Vaticanus and its relation to Codex Sinaiticus. It presents observations that point towards an origin in Caesarea/Palaestina. Although most authors who have written about the relation and origin of Codex Vaticanus and Codex Sinaiticus seem to be convinced about their view, it is, as even some quite determined authors admit, a matter of interpretation of some details. In this situation, additional observations may be welcome.
A new small marine Diploneis species (Diploneidaceae, Bacillariophyta) from Guangdong Province, China
Authors: WU WEIWEI , SUN LIN , LI XUESONG , LIANG JUNRONG , GAO YAHUI , CHEN CHANGPING
Published: September 2022

A Diploneis species new to science is described here, collected from an intertidal sandy beach in Guangdong Province, China. The species has morphological features such as small elongated-elliptical valves, a linear longitudinal canal open outside by one row of C-shaped areolae throughout the whole length, a straight and simple raphe, alveoli occluded externally by a vola and internally by a thin hymen, perforations arranged in a hexagonal array that occupy approximately one-fourth to one-sixth of the valve width, and the other part of the alveolate striae covered by a broadly semilune hyaline area at the middle of the valve. Differences with other similar taxa of this genus are discussed.
Impatiens piufanensis var. villosa (Balsaminaceae), a new variety from Hubei and Hunan, China
Authors: DING SHI-XIONG , LIU HUI-HUA , WANG BEN-ZHONG , PENG SHUAI , JIANG HUI , MUTIE FREDRICK MUNYAO , WASWA EMMANUEL NYONGESA , HU GUANG-WAN
Published: September 2022

Impatiens piufanensis var. villosa, a new variety of Balsaminaceae from Hubei and Hunan, China, is described and illustrated here. Morphologically, it can be distinguished from the nominate variety by its stem, leaves, lateral sepals, lower sepal and the midvein of upper petal covered by dense white villus and puberulent peduncles and pedicels. The phylogenetic analysis based on ITS sequence also supports its treatment as a new variety of I. piufanensis.
The green lacewings of Pakistan (Neuroptera: Chrysopidae): a faunal review with new records of genera and species
Authors: HASSAN MUHAMMAD ASGHAR , LIU XINGYUE
Published: September 2022

A review of the present knowledge on the taxonomy and distribution of the green lacewings (Neuroptera: Chrysopidae) from Pakistan is presented. So far, 19 species are known to occur in Pakistan including three species newly recorded, i.e., Italochrysa aequalis (Walker, 1853), Italochrysa everetti (van der Weele, 1909), and Tumeochrysa indica Needham, 1909. In addition, there are five undetermined species of Apertochrysa Tjeder, 1966 not determined in this paper. We here describe/re-describe 16 species of the 24 Pakistani green lacewing species. Two new combinations are proposed: Apertochrysa murreensis (Tjeder, 1963) comb. nov., and Apertochrysa vartianorum (Hölzel, 1973) comb. nov. The genus Tumeochrysa Needham, 1909 is recorded for the first time in Pakistan. Identification keys are provided to all known genera and species that occur in Pakistan. Additionally, for each species, taxonomic notes, diagnosis, a complete list of synonyms, and distribution maps are provided.
Review of the subgenus Euthraulus (Ephemeroptera, Leptophlebiidae, genus Choroterpes) from the Western Ghats (India)
Authors: KLUGE NIKITA , SRINIVASAN PANDIARAJAN , VASANTH M. , SIVARUBAN T. , BARATHY S. , ISACK RAJASEKARAN
Published: September 2022

The subgeneric name Monochoroterpes Kluge & Jacobus 2015 is regarded here to be a junior synonym of Euthraulus Barnard 1932 (syn. n.), based on a wider understanding of more species from each group. Based on male and female imagines reared from larvae, the following species are described or re-described: Choroterpes (Euthraulus) alagarensis Dinakaran et al. 2009, Ch. (E.) armillatus sp. n., Ch. (E.) latus sp. n., Ch. (E.) atelobranchis sp. n., Ch. (E.) unicolor sp. n., Ch. (E.) nambiyarensis Selva-Kumar et al. 2013, Ch. (E.) angustifolius sp. n. and Ch. (E.) nandini Selvakumar & Sivaramakrishnan 2015. All these species are distributed in southern India. One of them, Ch. (E.) latus sp. n., was collected in a plains river, Vaigai, in Madurai and in several localities in Sri Lanka, including a plains river, Malvatu, in Anuradhapura; formerly it was confused with another Ceylonese species, Ch. (E.) signatus (Hagen 1858). Here the characters separating Ch. (E.) signatus from Ch. (E.) latus sp. n. are reported and illustrated. Other species listed above were collected in mountain rivers in south of Western Ghats. One more species of Euthraulus is described without a formal name based a on mature male larva collected near Mumbai. The species description of Choroterpes (Euthraulus) kalladaensis Rekha et al. 2019 was based on specimens belonging both to Indialis rossi and Ch. (E.) angustifolius; since the type specimens are lost, and the description is based mainly on characters of Indialis rossi, we propose the following synonymy: Indialis rossi Peters 1975 = Choroterpes (Euthraulus) kalladaensis Rekha et al. 2019, syn. n.
Redescription of Banyutus cubitalis (Navás, 1914) (Neuroptera, Myrmeleontidae) and key to antlion genera in tribe Nemoleontini from India
Authors: SURYANARAYANAN THANGALAZHI BALAKRISHNAN , BIJOY CHENTHAMARAKSHAN , ÁBRAHÁM LEVENTE
Published: September 2022

The male and female of Banyutus cubitalis (Navás, 1914), a poorly known nemoleontid species from India was redescribed and illustrated based on the specimens collected for the first time from Southern Western Ghats (new faunistic records). Also, a key to the antlion genera in Nemoleontini tribe from India were provided.
The Little Fog Dragon—a new genus of Mountain Lichen Katydid (Orthoptera: Tettigoniidae: Phaneropterinae: Dysoniini) from the Serra do Sol, Roraima, Brazil
Authors: DE MELLO MENDES DIEGO MATHEUS
Published: September 2022

The Dysoniini tribe (Phaneropterinae: Tettigoniidae) currently comprises 15 genera and 85 species with a Neotropical distribution. The group is notable for its representatives possessing morphological adaptations to camouflage with lichens and fungi. In this work, the new genus Nebulodraculus gen. nov. is described with two species included: Nebulodraculus marioi sp. nov. (type species) and Nebulodraculus holgeri (Cadena-Castañeda, 2013) comb. nov. (transferred from Lichenodraculus Braun, 2011), known from mountainous environments in northern Brazil and Venezuela. Pictorial keys to the species of the genus, maps and natural history observations are provided.