bluuebunny/crossref_metadata_2025 huggingface dataset:
A High Performance All-Textile Wearable Antenna for Wristband Application
Authors: Ejaz Asma , Jabeen Iqra , Khan Zia Ullah , Alomainy Akram , Aljaloud Khaled , Alqahtani Ali H. , Hussain Niamat , Hussain Rifaqat , Amin Yasar
Published: June 2023

A compact, conformal, all-textile wearable antenna is proposed in this paper for the 2.45 GHz ISM (Industrial, Scientific and Medical) band. The integrated design consists of a monopole radiator backed by a 2 × 1 Electromagnetic Band Gap (EBG) array, resulting in a small form factor suitable for wristband applications. An EBG unit cell is optimized to work in the desired operating band, the results of which are further explored to achieve bandwidth maximization via floating EBG ground. A monopole radiator is made to work in association with the EBG layer to produce the resonance in the ISM band with plausible radiation characteristics. The fabricated design is tested for free space performance analysis and subjected to human body loading. The proposed antenna design achieves bandwidth of 2.39 GHz to 2.54 GHz with a compact footprint of 35.4 × 82.4 mm2. The experimental investigations reveal that the reported design adequately retains its performance while operating in close proximity to human beings. The presented Specific Absorption Rate (SAR) analysis reveals 0.297 W/kg calculated at 0.5 W input power, which certifies that the proposed antenna is safe for use in wearable devices.
Accurate Stride-Length Estimation Based on LT-StrideNet for Pedestrian Dead Reckoning Using a Shank-Mounted Sensor
Authors: Li Yong , Zeng Guopei , Wang Luping , Tan Ke
Published: June 2023

Pedestrian dead reckoning (PDR) is a self-contained positioning technology and has been a significant research topic in recent years. Pedestrian-stride-length estimation is the core part of the PDR system and directly affects the performance of the PDR. The current stride-length-estimation method is difficult to adapt to changes in pedestrian walking speed, which leads to a rapid increase in the error of the PDR. In this paper, a new deep-learning model based on long short-term memory (LSTM) and Transformer, LT-StrideNet, is proposed to estimate pedestrian-stride length. Next, a shank-mounted PDR framework is built based on the proposed stride-length-estimation method. In the PDR framework, the detection of pedestrian stride is achieved by peak detection with a dynamic threshold. An extended Kalman filter (EKF) model is adopted to fuse the gyroscope, accelerometer, and magnetometer. The experimental results show that the proposed stride-length-estimation method can effectively adapt to changes in pedestrian walking speed, and our PDR framework has excellent positioning performance.
A False Trigger-Strengthened and Area-Saving Power-Rail Clamp Circuit with High ESD Performance
Authors: Ma Boyang , Chen Shupeng , Wang Shulong , Qian Lingli , Han Zeen , Huang Wei , Fu Xiaojun , Liu Hongxia
Published: June 2023

A power clamp circuit, which has good immunity to false trigger under fast power-on conditions with a 20 ns rising edge, is proposed in this paper. The proposed circuit has a separate detection component and an on-time control component which enable it to distinguish between electrostatic discharge (ESD) events and fast power-on events. As opposed to other on-time control techniques, instead of large resistors or capacitors, which can cause a large occupation of the layout area, we use a capacitive voltage-biased p-channel MOSFET in the on-time control part of the proposed circuit. The capacitive voltage-biased p-channel MOSFET is in the saturation region after the ESD event is detected, which can serve as a large equivalent resistance (~106 Ω) in the structure. The proposed power clamp circuit offers several advantages compared to the traditional circuit, such as having at least 70% area savings in the trigger circuit area (30% area savings in the whole circuit area), supporting a power supply ramp time as fast as 20 ns, dissipating the ESD energy more cleanly with little residual charge, and recovering faster from false triggers. The rail clamp circuit also offers robust performance in an industry-standard PVT (process, voltage, and temperature) space and has been verified by the simulation results. Showing good performance of human body model (HBM) endurance and high immunity to false trigger, the proposed power clamp circuit has great potential for application in ESD protection.
Comparison of Circular and Rectangular-Shaped Electrodes for Electrical Impedance Myography Measurements on Human Upper Arms
Authors: Ahad Mohammad A. , Baidya Somen , Tarek Md. Nurul
Published: June 2023

Electrical Impedance Myography (EIM) is a painless, noninvasive approach for assessing muscle conditions through the application of a high-frequency, low-intensity current to the muscle region of interest. However, besides muscle properties, EIM measurements vary significantly with changes in some other anatomical properties such as subcutaneous skin-fat (SF) thickness and muscle girth, as well as non-anatomical factors, such as ambient temperature, electrode shape, inter-electrode distance, etc. This study has been conducted to compare the effects of different electrode shapes in EIM experiments, and to propose an acceptable configuration that is less dependent on factors other than the cellular properties of the muscle. Initially, a finite element model with two different kinds of electrode shapes, namely, rectangular (the conventional shape) and circular (the proposed shape) was designed for a subcutaneous fat thickness ranging from 5 mm to 25 mm. The study concludes, based on the FEM study, that replacing the conventional electrodes with our proposed electrodes can decrease the variation in EIM parameters due to changes in skin-fat thickness by 31.92%. EIM experiments on human subjects with these two kinds of electrode shapes validate our finite element simulation results, and show that circular electrodes can improve EIM effectiveness significantly, irrespective of muscle shape variation.
AlGaN/GaN Metal Oxide Semiconductor High-Electron Mobility Transistors with Annealed TiO2 as Passivation and Dielectric Layers
Authors: Lin Yu-Shyan , Lu Chi-Che
Published: June 2023

This paper reports on improved AlGaN/GaN metal oxide semiconductor high-electron mobility transistors (MOS-HEMTs). TiO2 is used to form the dielectric and passivation layers. The TiO2 film is characterized using X-ray photoemission spectroscopy (XPS), Raman spectroscopy, and transmission electron microscopy (TEM). The quality of the gate oxide is improved by annealing at 300 °C in N2. Experimental results indicate that the annealed MOS structure effectively reduces the gate leakage current. The high performance of the annealed MOS-HEMTs and their stable operation at elevated temperatures up to 450 K is demonstrated. Furthermore, annealing improves their output power characteristics.
Antimicrobial Resistance and Antimicrobial Stewardship: Before, during and after the COVID-19 Pandemic
Authors: Khaznadar Omar , Khaznadar Farah , Petrovic Ana , Kuna Lucija , Loncar Ana , Omanovic Kolaric Tea , Mihaljevic Vjera , Tabll Ashraf A. , Smolic Robert , Smolic Martina
Published: June 2023

Antimicrobial resistance (AMR) is an ongoing phenomenon. It is a significant public health issue that has existed long before the coronavirus disease of 2019 (COVID-19) pandemic. It develops as microorganisms undergo genetic mutations that allow them to survive despite antimicrobial treatment. This process is highly associated with excessive and often unnecessary antimicrobial pharmacotherapy, which was often discussed during the COVID-19 pandemic. This article explores how the pandemic has affected antimicrobial stewardship by shifting the focus away from antimicrobial resistance, as well as the impact of enhanced antibiotic usage and measures such as lockdowns, mandatory testing and vaccination on antimicrobial resistance. Although these measures were regarded as successful in terms of limiting the pandemic, they have significantly contributed to an already escalating AMR issue. Outpatient methods in primary care and intensive care units aiming to prevent severe COVID-19 disease have contributed to the spread of multidrug-resistant bacteria, while laboratories burdened with COVID-19 testing have indirectly interrupted the detection of these bacteria. In this review, we summarize the pathogens whose AMRe has been greatly affected by COVID-19 measures and emphasize the importance of efficient antimicrobial stewardship in future pandemic and non-pandemic states to promote the responsible use of antibiotics and minimize AMR.
Identification, Characterization, and Genome Analysis of Two Novel Temperate Pseudomonas protegens Phages PseuP_222 and PseuP_224
Authors: Morozova Vera , Kozlova Yuliya , Tikunov Artem , Babkin Igor , Ushakova Tatyana , Bardasheva Alevtina , Jdeed Ghadeer , Zhirakovskaya Elena , Mogileva Alina , Netesov Sergei , Tikunova Nina
Published: June 2023

Two novel P. protegens bacteriophages PseuP_222 and Pseu_224 and their host P. protegens CEMTC 4060 were isolated from the same sample (Inya river, Siberia). Both phages have siphovirus morphology and belong to lambdoid phages. Comparative genome analysis revealed a low nucleotide and amino acid sequence similarity of PseuP_222 and PseuP_224 between themselves, and between them and other lambdoid phages. Bioinformatics analysis indicated that PseuP_222 and PseuP_224 are members of a genetically diverse group of phages of environmental Pseudomonas spp.; this group is distant from a large group of P. aeruginosa phages. In phylogenetic trees, the positioning of the terminase large subunits, major capsid proteins, tail tape measure proteins, and CI-like repressors of PseuP_222 and PseuP_224 were remote and changed relative to those of the Escherichia lambda phage and lambdoid phages of Pseudomonas spp. However, the nucleoid-associated protein NdpA/YejK and P5-like structural protein from both phages showed high similarity and were not found in lambda phage and other lambdoid phages of Pseudomonas spp. Substantial divergences of the PseuP_222 and PseuP_224 genomes and proteomes indicated that the evolutionary history of these phages was mostly independent and they probably began to use one host only recently.
The Lanthipeptide Synthetase-like Protein CA_C0082 Is an Effector of Agr Quorum Sensing in Clostridium acetobutylicum
Authors: Humphreys Jonathan R. , Bean Zak , Twycross Jamie , Winzer Klaus
Published: June 2023

Lanthipeptide synthetases are present in all domains of life. They catalyze a crucial step during lanthipeptide biosynthesis by introducing thioether linkages during posttranslational peptide modification. Lanthipeptides have a wide range of functions, including antimicrobial and morphogenetic activities. Intriguingly, several Clostridium species contain lanthipeptide synthetase-like genes of the class II (lanM) family but lack other components of the lanthipeptide biosynthetic machinery. In all instances, these genes are located immediately downstream of putative agr quorum sensing operons. The physiological role and mode of action of the encoded LanM-like proteins remain uncertain as they lack conserved catalytic residues. Here we show for the industrial organism Clostridium acetobutylicum that the LanM-like protein CA_C0082 is not required for the production of active AgrD-derived signaling peptide but nevertheless acts as an effector of Agr quorum sensing. Expression of CA_C0082 was shown to be controlled by the Agr system and is a prerequisite for granulose (storage polymer) formation. The accumulation of granulose, in turn, was shown to be required for maximal spore formation but also to reduce early solvent formation. CA_C0082 and its putative homologs appear to be closely associated with Agr systems predicted to employ signaling peptides with six-membered ring structures and may represent a new subfamily of LanM-like proteins. This is the first time their contribution to bacterial Agr signaling has been described.
Microbiological Characterization of the Biofilms Colonizing Bioplastics in Natural Marine Conditions: A Comparison between PHBV and PLA
Authors: Marín Anna , Feijoo Patricia , de Llanos Rosa , Carbonetto Belén , González-Torres Pedro , Tena-Medialdea José , García-March José R. , Gámez-Pérez José , Cabedo Luis
Published: June 2023

Biodegradable polymers offer a potential solution to marine pollution caused by plastic waste. The marine biofilms that formed on the surfaces of poly(lactide acid) (PLA) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) were studied. Bioplastics were exposed for 6 months to marine conditions in the Mediterranean Sea, and the biofilms that formed on their surfaces were assessed. The presence of specific PLA and PHBV degraders was also studied. PHBV showed extensive areas with microbial accumulations and this led to higher microbial surface densities than PLA (4.75 vs. 5.16 log CFU/cm2). Both polymers’ surfaces showed a wide variety of microbial structures, including bacteria, fungi, unicellular algae and choanoflagellates. A high bacterial diversity was observed, with differences between the two polymers, particularly at the phylum level, with over 70% of bacteria affiliated to three phyla. Differences in metagenome functions were also detected, revealing a higher presence of proteins involved in PHBV biodegradation in PHBV biofilms. Four bacterial isolates belonging to the Proteobacteria class were identified as PHBV degraders, demonstrating the presence of species involved in the biodegradation of this polymer in seawater. No PLA degraders were detected, confirming its low biodegradability in marine environments. This was a pilot study to establish a baseline for further studies aimed at comprehending the marine biodegradation of biopolymers.
Nannochloris sp. Microalgae Strain for Treatment of Dairy Wastewaters
Authors: Paulenco Anca , Vintila Alin Cristian Nicolae , Vlaicu Alexandru , Ciltea-Udrescu Mihaela , Galan Ana-Maria
Published: June 2023

This paper focuses on a process for dairy wastewater treatment by mixotrophic cultivation of microalgae Nannochloris sp., using cheese whey obtained as a side flow from cheese production as an organic carbon source. The microalgae samples were prepared by adding to the standard growth medium increasing amounts of cheese whey, calculated to ensure a lactose concentration between 0 and 10 g/L. The samples were incubated at a constant temperature of 28 °C and 175 rpm stirring speed for a total time of seven days. Two LED (Light Emitting Diode) illumination schemes were applied in order to assess the effect of this parameter on microalgae development and bioactive compound accumulation: continuous illumination (light stress) versus alternative cycles of 12 h light—12 h dark (day–night cycle). The growth medium was analyzed before and after microalgae cultivation in order to determine the reduction of carbon, nitrogen, and phosphorus. The results obtained for this process, after a seven-day cultivation period, were as follows: reduction of 99–100% of lactose from the growth medium, up to 96% reduction in chemical oxygen demand, up to 91% reduction in nitrogen content, and up to 70% reduction in phosphorus content.
New Insights into the Role and Therapeutic Potential of Heat Shock Protein 70 in Bovine Viral Diarrhea Virus Infection
Authors: Chen Nannan , Bai Tongtong , Wang Shuang , Wang Huan , Wu Yue , Liu Yu , Zhu Zhanbo
Published: June 2023

Bovine viral diarrhea virus (BVDV), a positive-strand RNA virus of the genus Pestivirus in the Flaviviridae family, is the causative agent of bovine viral diarrhea–mucosal disease (BVD-MD). BVDV’s unique virion structure, genome, and replication mechanism in the Flaviviridae family render it a useful alternative model for evaluating the effectiveness of antiviral drugs used against the hepatitis C virus (HCV). As one of the most abundant and typical heat shock proteins, HSP70 plays an important role in viral infection caused by the family Flaviviridae and is considered a logical target of viral regulation in the context of immune escape. However, the mechanism of HSP70 in BVDV infection and the latest insights have not been reported in sufficient detail. In this review, we focus on the role and mechanisms of HSP70 in BVDV-infected animals/cells to further explore the possibility of targeting this protein for antiviral therapy during viral infection.
Recent Trends in Antimicrobial Resistance among Anaerobic Clinical Isolates
Authors: Reissier Sophie , Penven Malo , Guérin François , Cattoir Vincent
Published: June 2023

Anaerobic bacteria are normal inhabitants of the human commensal microbiota and play an important role in various human infections. Tedious and time-consuming, antibiotic susceptibility testing is not routinely performed in all clinical microbiology laboratories, despite the increase in antibiotic resistance among clinically relevant anaerobes since the 1990s. β-lactam and metronidazole are the key molecules in the management of anaerobic infections, to the detriment of clindamycin. β-lactam resistance is usually mediated by the production of β-lactamases. Metronidazole resistance remains uncommon, complex, and not fully elucidated, while metronidazole inactivation appears to be a key mechanism. The use of clindamycin, a broad-spectrum anti-anaerobic agent, is becoming problematic due to the increase in resistance rate in all anaerobic bacteria, mainly mediated by Erm-type rRNA methylases. Second-line anti-anaerobes are fluoroquinolones, tetracyclines, chloramphenicol, and linezolid. This review aims to describe the up-to-date evolution of antibiotic resistance, give an overview, and understand the main mechanisms of resistance in a wide range of anaerobes.
Genetic Mechanism of Structurally Controlled Dolomites Derived from Seawater-Hydrothermal Mixed Fluids—A Case Study from Middle Permian, Central Sichuan Basin, South China
Authors: Gao Jinliang , Zheng Haofu , Liu Bo , Pan Lei , Li Rangbin , Wu Junfeng , Yang Xiangyang , Tang Hailei , Dong Yixin
Published: June 2023

Dolomite bodies in the Middle Permian of the central Sichuan Basin have been reported as favorable natural gas reservoirs. The Middle Permian dolomite consists of three types of recrystallized dolomite (Rd1, Rd2, and Rd3) and one type of dolomite cement (Sd). Rd1 might be formed as the primary mineral along the calcite in the original sea-water. Its δ13C value and 87Sr/86Sr ratio, consistent with those of marine limestone and Permian seawater, support that the dolomitizing fluid for Rd1 was Permian seawater preserved in the strata. Rd2 consists of fine to medium (50 μm to 250 μm) and planar to curved crystals. Geochemical indicators (slightly high 87Sr/86Sr ratio, similar rare earth element patterns, negative δ18O, slightly high salinity) confirm that the dolomitizing fluid of Rd2 was mainly Permian seawater during shallow burial, with a small number of hydrothermal fluids. Rd3 and Sd are featured by very large (>250 μm), curved crystals, and high-temperature, high-salinity, and obviously positive Eu anomalies, suggesting that their diagenetic fluids were mainly hydrothermal fluids from deep. Additionally, inherited carbon sources and the 87Sr/86Sr ratios of some samples fall within the range of Permian seawater distribution, confirming the contribution of Permian seawater. ELIP activity caused the formation of this dolomite through the mixing of seawater and hydrothermal fluids. The main fluid circulation channels were activated basement faults, epigenetic karst pores, and shallowly buried high-permeability strata. During the peak period of ELIP activity, the continuous upwelling of deep hydrothermal fluids led to the continuous formation of Rd2, Rd3, and Sd. The dolomitization fluid of Rd2 was mainly composed of seawater and featured a certain lateral extension, which was away from faults. Rd3 and Sd are mainly distributed along the fault system, and excessive dolomitization caused by the hydrothermal activity, to some extent, inhibited the lateral movement of hydrothermal fluids. This study provides a good example for exploring the genetic mechanism and distribution pattern of structurally controlled dolomites under a volcanic activity background.
Study on the Combined Behaviour of Montmorillonite and Carbonate Mineralizing Bacteria on Lead Retention and Fixation
Authors: Dai Qunwei , Wang Weifu , Xu Fengqin , Zhao Yulian , Zhou Lei , Wang Lihui , Jiang Ruiyang
Published: June 2023

Heavy metal pollution management is a global environmental problem that poses a great threat to the ecological environment. Adsorption and biomineralization are considered to be the two most promising heavy metal pollution remediation techniques among the numerous available ones. In this work, a combined heavy metal removal system was constructed employing the adsorption of montmorillonite and the mineralization of carbonate mineralizing bacteria to expand their application potential for controlling heavy metal water pollution. Analysis of changes in pH, CO32− concentration, Pb2+ concentration in the liquid phase, and changes in microscopic morphology, mineral phase, and functional group on sedimentary minerals was done to study the retention and fixation behaviour of montmorillonite and carbonate mineralizing bacteria on Pb2+. The results show that the liquid phase pH may be slightly altered by the ions dissolved in montmorillonite. Based on the conditions of montmorillonite and carbonate mineralizing bacteria functioning separately, the elimination of Pb2+ may reach 35.31% and 45.75%, respectively. However, when montmorillonite is combined with carbonate mineralizing bacteria, which is the heavy metal removal system constructed in this study, montmorillonite can buffer part of the Pb2+ rapidly and reduce its biotoxicity. Subsequently, these buffered Pb2+ are gradually desorbed by carbonate mineralizing bacteria and removed by the effect of mineralized deposition. Results from SEM, FTIR, and XRD indicate that Pb2+ is primarily removed from the liquid phase as rod-shaped PbCO3. It is worth noting that this process is able to significantly increase the removal of Pb2+ up to 90.06%. In addition, the presence of carbonate mineralizing bacteria can increase montmorillonite’s desorption rate to over 81%, greatly enhancing its capacity for reuse. Therefore, our research work contributes to expanding the potential of montmorillonite and carbonate mineralizing bacteria in the treatment of heavy metal-polluted waters.
Development and Evaluation of a Mobile Application with Augmented Reality for Guiding Visitors on Hiking Trails
Authors: Silva Rute , Jesus Rui , Jorge Pedro
Published: June 2023

Tourism on the island of Santa Maria, Azores, has been increasing due to its characteristics in terms of biodiversity and geodiversity. This island has several hiking trails; the available information can be consulted in pamphlets and physical placards, whose maintenance and updating is difficult and expensive. Thus, the need to improve the visitors’ experience arises, in this case, by using the technological means currently available to everyone: a smartphone. This paper describes the development and evaluation of the user experience of a mobile application for guiding visitors on said hiking trails, as well as the design principles and main issues observed during this process. The application is based on an augmented reality interaction model providing visitors with an interactive and recreational experience through Augmented Reality in outdoor environments (without additional marks in the physical space and using georeferenced information), helping in navigation during the route and providing updated information with easy maintenance. For the design and evaluation of the application, two studies were carried out with users on-site (Santa Maria, Azores). The first had 77 participants, to analyze users and define the application’s characteristics, and the second had 10 participants to evaluate the user experience. The feedback from participants was obtained through questionnaires. In these questionnaires, an average SUS (System Usability Scale) score of 83 (excellent) and positive results in the UEQ (User Experience Questionnaire) were obtained.
Influence of Thermal and Flash-Lamp Annealing on the Thermoelectrical Properties of Cu2ZnSnS4 Nanocrystals Obtained by “Green” Colloidal Synthesis
Authors: Havryliuk Yevhenii , Dzhagan Volodymyr , Karnaukhov Anatolii , Selyshchev Oleksandr , Hann Julia , Zahn Dietrich R. T.
Published: June 2023

The problem with waste heat in solar panels has stimulated research on materials suitable for hybrid solar cells, which combine photovoltaic and thermoelectric properties. One such potential material is Cu2ZnSnS4 (CZTS). Here, we investigated thin films formed from CZTS nanocrystals obtained by “green” colloidal synthesis. The films were subjected to thermal annealing at temperatures up to 350 °C or flash-lamp annealing (FLA) at light-pulse power densities up to 12 J/cm2. The range of 250–300 °C was found to be optimal for obtaining conductive nanocrystalline films, for which the thermoelectric parameters could also be determined reliably. From phonon Raman spectra, we conclude that in this temperature range, a structural transition occurs in CZTS, accompanied by the formation of the minor CuxS phase. The latter is assumed to be a determinant for both the electrical and thermoelectrical properties of CZTS films obtained in this way. For the FLA-treated samples, the film conductivity achieved was too low to measure the thermoelectric parameters reliably, although the partial improvement of the CZTS crystallinity is observed in the Raman spectra. However, the absence of the CuxS phase supports the assumption of its importance with respect to the thermoelectric properties of such CZTS thin films.
Environmentally Benign pSOFC for Emissions-Free Energy: Assessment of Nickel Network Resistance in Anodic Ni/BCY15 Nanocatalyst
Authors: Gabrovska Margarita , Nikolova Dimitrinka , Kolev Hristo , Karashanova Daniela , Tzvetkov Peter , Burdin Blagoy , Mladenova Emiliya , Vladikova Daria , Tabakova Tatyana
Published: June 2023

Yttrium-doped barium cerate (BCY15) was used as ceramic matrix to obtain Ni/BCY15 anode cermet for application in proton-conducting solid oxide fuel cells (pSOFC). Ni/BCY15 cermets were prepared in two different types of medium, namely deionized water (W) and anhydrous ethylene glycol (EG) using wet chemical synthesis by hydrazine. An in-depth analysis of anodic nickel catalyst was made aiming to elucidate the effect of anode tablets’ preparation by high temperature treatment on the resistance of metallic Ni in Ni/BCY15-W and Ni/BCY15-EG anode catalysts. On purpose reoxidation upon high-temperature treatment (1100 °C for 1 h) in air ambience was accomplished. Detailed characterization of reoxidized Ni/BCY15-W-1100 and Ni/BCY15-EG-1100 anode catalysts by means of surface and bulk analysis was performed. XPS, HRTEM, TPR, and impedance spectroscopy measurements experimentally confirmed the presence of residual metallic Ni in the anode catalyst prepared in ethylene glycol medium. These findings were evidence of strong metal Ni network resistance to oxidation in anodic Ni/BCY15-EG. Enhanced resistance of the metal Ni phase contributed to a new microstructure of the Ni/BCY15-EG-1100 anode cermet getting more stable to changes that cause degradation during operation.
Focused Ultrasound-Mediated Blood–Brain Barrier Opening Best Promotes Neuroimmunomodulation through Brain Macrophage Redistribution
Authors: Kline-Schoder Alina R. , Noel Rebecca L. , Phatnani Hemali , Menon Vilas , Konofagou Elisa E.
Published: June 2023

Neuroimmunomodulation is a promising form of drug-free treatment for neurological diseases ranging from Alzheimer’s disease to depression. The evidence supporting the efficacy of focused ultrasound (FUS) neuroimmunomodulation is encouraging; however, the method has yet to be standardized, and its mechanism remains poorly understood. Methods of FUS neuroimmunomodulation can be categorized into three paradigms based on the parameters used. In the first paradigm, focused ultrasound blood–brain barrier opening (FUS-BBBO) combines FUS with microbubbles (MB) to transiently and safely induce BBB opening. In the second paradigm, focused ultrasound neuromodulation (FUS-N) harnesses the acoustic effects of FUS alone (without MB). In the third paradigm, focused ultrasound with microbubbles without BBBO (FUS + MB) combines MB with FUS below the BBBO pressure threshold—harnessing the mechanical effects of FUS without opening the barrier. Due to the recent evidence of brain macrophage modulation in response to FUS-BBBO, we provide the first direct comparison of brain macrophage modulation between all three paradigms both in the presence and absence of Alzheimer’s disease (AD) pathology. Flow cytometry and single-cell sequencing are employed to identify FUS-BBBO as the FUS paradigm, which maximizes brain macrophage modulation, including an increase in the population of neuroprotective, disease-associated microglia and direct correlation between treatment cavitation dose and brain macrophage phagocytosis. Next, we combine spatial and single-cell transcriptomics with immunohistochemical validation to provide the first characterization of brain macrophage distribution in response to FUS-BBBO. Given their relevance within neurodegeneration and perturbation response, we emphasize the analysis of three brain macrophage populations—disease- and interferon-associated microglia and central-nervous-system-associated macrophages. We find and validate the redistribution of each population with an overall trend toward increased interaction with the brain–cerebrospinal fluid barrier (BCSFB) after FUS-BBBO, an effect that is found to be more pronounced in the presence of disease pathology. This study addresses the prior lack of FUS neuroimmunomodulation paradigm optimization and mechanism characterization, identifying that FUS-BBBO best modulates brain macrophage response via complex redistribution.
Impact of COVID-19 Restriction on Weight, Physical Activity, Diet and Psychological Distress on People with PCOS
Authors: McGowan Margaret , Lim Siew , O’Reilly Sharleen L. , Harrison Cheryce L. , Enticott Joanne , Teede Helena , Cowan Stephanie , Moran Lisa J.
Published: June 2023

Background: People with polycystic ovary syndrome (PCOS) have higher weight gain and psychological distress compared to those without PCOS. While COVID-19 restrictions led to population level adverse changes in lifestyle, weight gain and psychological distress, their impact on people with PCOS is unclear. The aim of this study was to investigate the impact the 2020 COVID-19 restrictions had on weight, physical activity, diet and psychological distress for Australians with PCOS. Methods: Australian reproductive-aged women participated in an online survey with assessment of weight, physical activity, diet and psychological distress. Multivariable logistic and linear regression were used to examine associations between PCOS and residential location with health outcomes. Results: On adjusted analysis, those with PCOS gained more weight (2.9%; 95% CI; 0.027–3.020; p = 0.046), were less likely to meet physical activity recommendations (OR 0.50; 95% CI; 0.32–0.79; p = 0.003) and had higher sugar-sweetened beverage intake (OR 1.74; 95% CI 1.10–2.75; p = 0.019) but no differences in psychological distress compared to women without PCOS. Conclusions: People with PCOS were more adversely affected by COVID-19 restrictions, which may worsen their clinical features and disease burden. Additional health care support may be necessary to assist people with PCOS to meet dietary and physical activity recommendations.
Gut Microbiota Modulation of Short Bowel Syndrome and the Gut–Brain Axis
Authors: Carter Jasmine , Bettag Jeffery , Morfin Sylvia , Manithody Chandrashekhara , Nagarapu Aakash , Jain Aditya , Nazzal Hala , Prem Sai , Unes Meghan , McHale Matthew , Lin Chien-Jung , Hutchinson Chelsea , Trello Grace , Jain Arti , Portz Edward , Verma Arun , Swiderska-Syn Marzena , Goldenberg Daniel , Kurashima Kento
Published: June 2023

Short bowel syndrome (SBS) is a condition that results from a reduction in the length of the intestine or its functional capacity. SBS patients can have significant side effects and complications, the etiology of which remains ill-defined. Thus, facilitating intestinal adaptation in SBS remains a major research focus. Emerging data supports the role of the gut microbiome in modulating disease progression. There has been ongoing debate on defining a “healthy” gut microbiome, which has led to many studies analyzing the bacterial composition and shifts that occur in gastrointestinal disease states such as SBS and the resulting systemic effects. In SBS, it has also been found that microbial shifts are highly variable and dependent on many factors, including the anatomical location of bowel resection, length, and structure of the remnant bowel, as well as associated small intestinal bacterial overgrowth (SIBO). Recent data also notes a bidirectional communication that occurs between enteric and central nervous systems called the gut–brain axis (GBA), which is regulated by the gut microbes. Ultimately, the role of the microbiome in disease states such as SBS have many clinical implications and warrant further investigation. The focus of this review is to characterize the role of the gut microbiota in short bowel syndrome and its impact on the GBA, as well as the therapeutic potential of altering the microbiome.
Ultra-Processed Food Consumption and Incidence of Obesity and Cardiometabolic Risk Factors in Adults: A Systematic Review of Prospective Studies
Authors: Mambrini Sara Paola , Menichetti Francesca , Ravella Simone , Pellizzari Marta , De Amicis Ramona , Foppiani Andrea , Battezzati Alberto , Bertoli Simona , Leone Alessandro
Published: June 2023

Ultra-processed foods (UPF) are energy-dense, nutritionally unbalanced products, low in fiber but high in saturated fat, salt, and sugar. Recently, UPF consumption has increased likewise the incidence of obesity and cardiometabolic diseases. To highlight a possible relationship, we conducted a systematic review of prospective studies from PubMed and Web of Science investigating the association between UPF consumption and the incidence of obesity and cardiometabolic risk factors. Seventeen studies were selected. Eight evaluated the incidence of general and abdominal obesity, one the incidence of impaired fasting blood glucose, four the incidence of diabetes, two the incidence of dyslipidemia, and only one the incidence of metabolic syndrome. Studies’ quality was assessed according to the Critical Appraisal Checklist for cohort studies proposed by the Joanna Briggs Institute. Substantial agreement emerged among the studies in defining UPF consumption as being associated with the incident risk of general and abdominal obesity. More limited was the evidence on cardiometabolic risk. Nevertheless, most studies reported that UPF consumption as being associated with an increased risk of hypertension, diabetes, and dyslipidemia. In conclusion, evidence supports the existence of a relationship between UPF consumption and the incidence of obesity and cardiometabolic risk. However, further longitudinal studies considering diet quality and changes over time are needed.
The Causal Effect of Dietary Composition on the Risk of Breast Cancer: A Mendelian Randomization Study
Authors: Dong Hao , Kong Xiangyi , Wang Xiangyu , Liu Qiang , Fang Yi , Wang Jing
Published: June 2023

Breast cancer has become the most common malignancy among women, posing a severe health risk to women worldwide and creating a heavy social burden. Based on current observational studies, the dietary factor may have a causal relationship with breast cancer. Therefore, exploring how dietary composition affects breast cancer incidence will provide nutrition strategies for clinicians and women. We performed a two-sample Mendelian randomization (MR) analysis to find the causal effect of four kinds of relative macronutrient intake (protein, carbohydrate, sugar, and fat) on the risk of breast cancer and its subtypes [Luminal A, Luminal B, Luminal B HER2-negative, HER2-positive, Triple-negative, Estrogen receptor (ER) positive, and ER-negative breast cancer]. The Mendelian randomization pleiotropy residual sum and outlier (MR-PRESSO) test, MR-Egger intercept test, Cochran’s Q statistic, funnel plot, and leave-one-out (Loo) analysis were all used in a sensitivity analysis to test the robustness of MR. Genetically, a higher relative protein intake was found as a protective factor for Luminal A and overall breast cancer, which was inconsistent with recent findings. A higher relative sugar intake could genetically promote the risk of Luminal B and HER2-positive breast cancer. Conclusions: A higher protein proportion in diet genetically reduces the risk of breast cancer, while higher relative sugar intake does the opposite.
A Microwell Device for the Efficient Generation of Arrays of Microtissues and Humanized Bone Marrow Micro-Ossicles
Authors: Futrega Kathryn , Shajib Md. Shafiullah , Robey Pamela G. , Doran Michael R.
Published: June 2023

(1) Background: There are no high-throughput microtissue platforms for generating bone marrow micro-ossicles. Herein, we describe a method for the assembly of arrays of microtissues from bone marrow stromal cells (BMSC) in vitro and their maturation into bone marrow micro-ossicles in vivo. (2) Methods: Discs with arrays of 50 microwells were used to assemble microtissues from 3 × 105 BMSCs each on a nylon mesh carrier. Microtissues were cultured in chondrogenic induction medium followed by hypertrophic medium in an attempt to drive endochondral ossification, and then they were implanted in NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice, where they were remodeled into bone marrow micro-ossicles. Mice were transplanted with 105 human umbilical cord blood CD34+ cells. (3) Results: Micro-ossicles contained more human CD45+ cells, but fewer human CD34+ progenitor cells than mouse marrow. Human hematopoietic progenitor cells cycle rapidly at non-physiological rates in mouse marrow, and reduced CD34+ cell content in micro-ossicles is consistent with the notion that the humanized niche better controls progenitor cell cycling. (4) Conclusions: Assembling microtissues in microwells, linked by a nylon membrane carrier, provides an elegant method to manufacture and handle arrays of microtissues with bone organ-like properties. More generally, this approach and platform could aid bridging the gap between in vitro microtissue manipulation and in vivo microtissue implantation.
Testing the Paradigm of Nuclear Many-Body Theory
Authors: Benhar Omar
Published: June 2023

Nuclear many-body theory is based on the tenet that nuclear systems can be accurately described as collections of point-like particles. This picture, while providing a remarkably accurate explanation of a wealth of measured properties of atomic nuclei, is bound to break down in the high-density regime, in which degrees of freedom other than protons and neutrons are expected to come into play. Valuable information on the validity of the description of dense nuclear matter in terms of nucleons, needed to firmly establish its limit of applicability, can be obtained from electron–nucleus scattering data at large momentum transfer and low energy transfer. The emergence of y-scaling in this kinematic region, unambiguously showing that the beam particles couple to high-momentum nucleons belonging to strongly correlated pairs, indicates that at densities as large as five times nuclear density—typical of the neutron star interior—nuclear matter largely behaves as a collection of nucleons.
Molecular and Serological Identification of Anaplasma marginale and Borrelia burgdorferi in Cattle and Ticks from Nuevo Leon, Northern Mexico
Authors: Ortiz-Ramírez José Ángel , Rodríguez-Rojas Jorge Jesús , Hernández-Escareño Jesús Jaime , Galan-Huerta Kame-A , Rebollar-Téllez Eduardo Alfonso , Moreno-Degollado Gustavo , Medina-De la Garza Carlos E. , Sánchez-Casas Rosa María , Fernández-Salas Ildefonso
Published: June 2023

Ticks and tick-borne diseases affect livestock productivity and cause significant economic losses. Therefore, surveillance of these pathogens and vectors is paramount to reducing these effects in livestock. This study aimed to identify Anaplasma marginale and Borrelia burgdorferi sensu lato in ticks collected from cattle. Molecular biology techniques were utilized to identify A. marginale for both types of samples, i.e., ticks and bovine blood. Serology of cattle using indirect immunofluorescence assay (IFA) was conducted to determine antibodies to B. burgdorferi s.l. from seven locations in Nuevo Leon, Mexico, between 2015 and 2017. From 404 bovines, 2880 ticks were collected: Rhipicephalus microplus (2391 females and 395 males), Amblyomma spp. (51 females and 42 males) and Dermacentor variabilis (1 female). Rhipicephalus microplus represented the largest specimens captured, with 96.7% within the seven study sites. PCR processed only 15% (442) of tick samples to identify A. marginale. Field genera proportions were followed to select testing tick numbers. Results showed that 9.9% (44/442) of A. maginale infected the pooled tick species, whereas the highest percent corresponded to 9.4% (38/404) in R. microplus. Regarding the molecular analysis of blood samples, 214 of 337 (63.5%) were positive for A. maginale. In each of the seven locations, at least one bovine sample tested positive for A. maginale. Borrelia burgdorferi s.l. was not found either in the ticks or serum samples. Two A.marginale DNA nucleotide sequences obtained in this study were deposited in the GenBank with the following accession numbers OR050501 cattle, and OR050500 R.microplus tick. Results of this work point to current distribution of bovine anaplasmosis in northern Mexico.
Flos populi (Male Inflorescence of Populus tomentosa Carrière) Aqueous Extract Suppresses Salmonella Pullorum Infection by Affecting T3SS-1
Authors: Zhang Wenting , Liang Guixing , Cheng Zhenyu , Guo Yunqing , Jiang Boda , Liu Tingjiang , Liao Weidong , Lu Qin , Wen Guoyuan , Zhang Tengfei , Luo Qingping
Published: June 2023

Pullorum disease, caused by Salmonella Pullorum (S. Pullorum), is one of the most serious infectious diseases in the poultry industry. Flos populi is traditionally used in Eastern Asian countries to treat various intestinal diseases. However, the anti-infection mechanism of Flos populi is not very clear. In this study, we evaluated the anti-infective effects on S. Pullorum of Flos populi aqueous extract (FPAE) in chickens. FPAE significantly reduced S. Pullorum growth in vitro. At the cellular level, FPAE reduced S. Pullorum adhesion and invasion on DF-1 cells but did not affect its intracellular survival or replication in macrophages. Further investigation revealed that FPAE inhibited the transcription of T3SS-1 genes, which is the main virulence factor that mediates S. Pullorum adhesion and invasion in host cells. The results suggest that the anti-infective effect of FPAE likely occurs through the inhibition of S. Pullorum T3SS-1, thereby impairing its ability to adhere to and invade cells. Further, we evaluated its therapeutic effect on animal models (Jianghan domestic chickens) and found that FPAE reduced the bacterial loads in organs and decreased the mortality and weight loss of infected chickens. Our findings provide novel insights into the potential development of FPAE against S. Pullorum as an effective anti-virulence therapeutic substitute for antibiotics.
Using Serological Markers for the Surveillance of Plasmodium vivax Malaria: A Scoping Review
Authors: Kartal Lejla , Mueller Ivo , Longley Rhea J.
Published: June 2023

The utilisation of serological surveillance methods for malaria has the potential to identify individuals exposed to Plasmodium vivax, including asymptomatic carriers. However, the application of serosurveillance varies globally, including variations in methodology and transmission context. No systematic review exists describing the advantages and disadvantages of utilising serosurveillance in various settings. Collation and comparison of these results is a necessary first step to standardise and validate the use of serology for the surveillance of P. vivax in specific transmission contexts. A scoping review was performed of P. vivax serosurveillance applications globally. Ninety-four studies were found that met predefined inclusion and exclusion criteria. These studies were examined to determine the advantages and disadvantages of serosurveillance experienced in each study. If studies reported seroprevalence results, this information was also captured. Measurement of antibodies serves as a proxy by which individuals exposed to P. vivax may be indirectly identified, including those with asymptomatic infections, which may be missed by other technologies. Other thematic advantages identified included the ease and simplicity of serological assays compared to both microscopy and molecular diagnostics. Seroprevalence rates varied widely from 0–93%. Methodologies must be validated across various transmission contexts to ensure the applicability and comparability of results. Other thematic disadvantages identified included challenges with species cross-reactivity and determining changes in transmission patterns in both the short- and long-term. Serosurveillance requires further refinement to be fully realised as an actionable tool. Some work has begun in this area, but more is required.
Molecular Investigations of Two First Brucella suis Biovar 2 Infections Cases in French Dogs
Authors: Girault Guillaume , Djokic Vitomir , Petot-Bottin Fathia , Perrot Ludivine , Thibaut Bourgoin , Sébastien Hoffmann , Vicente Acacia Ferreira , Ponsart Claire , Freddi Luca
Published: June 2023

Despite Brucella suis biovar 2’s (BSB2) active circulation in wildlife, no canine infections have been reported. The present paper is the first to describe two cases of BSB2 infections in French dogs. The first case occurred in 2020 and concerned a 13-year-old male neutered Border Collie with clinical signs of prostatitis. The urine culture revealed the excretion of significant levels of Brucella in the sample. The second case concerned a German Shepherd with bilateral orchitis, in which it was possible to detect Brucella colonies following neutering. HRM-PCR and classical biotyping methods classified both isolated strains as BSB2, in contrast to expected B. canis, which is usually the etiological agent of canine brucellosis in Europe. The wgSNP and MLVA analyses highlighted the genetic proximity of two isolates to BSB2 strains originating from wildlife. No pig farms were present in the proximity of either dog’s residence, ruling out potential spill over from infected pigs. Nevertheless, the dogs used to take walks in the surrounding forests, where contact with wildlife (i.e., wild boars or hares, or their excrements) was possible. These cases highlight the importance of adopting a One Health approach to control the presence of zoonotic bacteria in wild animals and avoid spillovers into domestic animals and, potentially, humans.