bluuebunny/crossref_metadata_2025 huggingface dataset:
Risk Factors Associated with Multimorbidity among Children Aged Under-Five Years in Sub-Saharan African Countries: A Scoping Review
Authors: Obasohan Phillips Edomwonyi , Walters Stephen J. , Jacques Richard , Khatab Khaled
Published: January 2023

Background/purpose: Globally, the prevalence of multimorbidity (defined as the cooccurrence of two or more diseases in an individual without reference to an index disease) is greater than 33%. Consequently, childhood multimorbidity, a growing public health concern in Low- and Middle-Income-Countries (LMICs), frequently has an impact on children’s health. Therefore, the aim of this scoping review was to locate and describe studies that investigate the association between socioeconomic, demographic, and environmental factors and the prevalence of multimorbidity among children aged under five years in Sub-Saharan Africa (SSA). Methods/Design: We searched MEDLINE, Cumulative Index to Nursing and Allied Health Literature (CINAHL), PubMed, Scopus, and Web of Science for papers written in English, and published between January 1990 and March 2022. The search included papers that focused on children aged under five years with multimorbidity from Sub-Saharan Africa and used classical regression methods in their analysis. Results: A total of 261 articles were identified. Out of the 66 articles selected for full-text reading, 60 were removed for various reasons. Therefore, data from a sample of six articles were finally extracted and reported in this study. The sample size for the 6 studies included ranged from 2343 to 193,065 children under five years of age. There were six distinct disease conditions (Pneumonia, diarrhoea, malaria, being overweight, stunting, and anaemia) analysed in the included studies. One of the studies had three concurrent diseases, while the other five studies had two current diseases as their multimorbidity outcome of interest. The prevalence of multimorbidity in these six studies ranged from 1.2% to 24.8%. Conclusions: The relatively few studies found in this research area is an indication of an evidence deficit/knowledge gap yearning to be filled to help policymakers in coming up with integrated multimorbidity care for children in SSA.
The Effect of Kosher Determinants of Beef on Its Color, Texture Profile and Sensory Evaluation
Authors: Żurek Jagoda , Rudy Mariusz , Stanisławczyk Renata , Duma-Kocan Paulina
Published: January 2023

Traditional ritual slaughter is an underlying practice in religious Jewish communities and it translates into eating in accordance with core beliefs. This paper aims to comprehensively analyze factors determining the kosher status of beef (sex, slaughter method, and muscle type) and assess their impact on selected quality properties, such as color or texture profile. Sensory evaluation was also performed. The muscles were obtained from 80 carcasses (50% of heifers and 50% of young bulls). The experimental results indicate that each studied kosherness determinant influenced beef quality properties. The process of koshering caused the darkening of beef and lowered the share of color parameters red (a*) and yellow (b*). The influence of the type of slaughter on the values of adhesiveness, gumminess, and chewiness of beef was confirmed; higher values were mostly obtained in the muscles of cattle from kosher slaughter. As to sensory evaluation, the study showed that in the case of muscle type, the sex and slaughter method positively influenced only some of the properties.
Analysing the Effect of Energy Intensity on Carbon Emission Reduction in Beijing
Authors: Li Gen , Zeng Shihong , Li Tengfei , Peng Qiao , Irfan Muhammad
Published: January 2023

Beijing has experienced rapid economic development since the reforms and opening up. However, the traditional development model based on excessive energy consumption has posed great challenges to the ecological environment. To curb environmental degradation and achieve sustainable social development, Beijing has proposed to achieve carbon neutrality by 2050. As an important indicator of energy consumption, it is necessary to clarify how energy intensity (EI) affects carbon emissions (CE) to achieve carbon neutrality in Beijing by 2050. This study first decomposes the drivers of CE in Beijing from 2010 to 2020 using the logarithmic mean Divisia index (LMDI) method and comparatively analyses the impact of EI on CE. Then, the spatial Dubin model (SDM) is used to analyse the spatial spillover effect of EI on CE at the regional level. Finally, the macro moderating role of economic development in the effect of EI on CE is analysed. The results show that the effect of EI has been the main driver of CE reduction in Beijing. Among the industrial sectors, manufacturing and transportation have had the greatest success in reducing CE through EI reduction. At the regional level, there is a spatial spillover effect of EI on CE, and the effect of carbon reduction through the spillover effect of EI is greater than the direct effect of EI. Economic factors have an enhanced moderating effect on the process of EI affecting CE, and this moderating effect has threshold properties.
Predictors of Healthcare Workers’ Compassionate Care Amid the COVID-19 Pandemic: A Cross-Sectional Study from Patients’ Perspective in Kelantan, Malaysia
Authors: Mohamed Noor Noorhidayu Monyati , Ibrahim Mohd Ismail , Hairon Suhaily Mohd , Mohd Zain Maizun , Satiman Mohd Saiful Nazri
Published: January 2023

Background: Compassionate care served by healthcare workers (HCWs) has been recognized as one of the most critical aspects of high-quality care. Unfortunately, there is still an unmet need for the assessment of compassionate care from the patient’s perspective. During the COVID-19 pandemic, many new rules were enacted to tackle the raging pandemic, which raised concerns about its effect on compassionate care. Methods: A cross-sectional study involving 315 patients from three public hospitals was conducted during the conditional movement control order (CMCO). A self-administered Malay version of the Relational Aspect of Care Questionnaire (RAC-QM) was used to assess compassionate care. Multiple linear regression was used to determine the predictors. Results: More than 90% of the patients were Malays, Muslims, and fell under the B40 household income category. Companions were present for 51.7% of the patients, but 75.2% had no visitors. All hospitals received scores of more than 90%. Occupation (student, p = 0.032), dependency level (total dependent, p < 0.001), and household income level (M40, p = 0.027) were the statistically significant predictors for compassionate care. Conclusions: The current study revealed that compassionate care to patients was not compromised during the pandemic. Patients with disabilities or financial constraints are more likely to experience less compassionate care, while students are generally more satisfied. This study may provide clues for hospital administrators and policymakers regarding the vulnerable group of patients. It also provides opportunities for future research to study the perspective of HCWs.
Parenting Styles, Food Parenting Practices, Family Meals, and Weight Status of African American Families
Authors: Ardakani Azam , Monroe-Lord Lillie , Wakefield Dorothy , Castor Chimene
Published: January 2023

Parents influence adolescents’ weight status through different strategies used in the home environment, including parenting styles (PSs), food parenting practices (FPPs), and family meal frequency. As the prevalence of obesity is higher among African American adolescents, investigation of which parental strategies serve as an adjustable factor for the prevention of obesity is critical. First, this study aims to examine the relationship between the different parenting influences and obesity statuses of both parents and 10–17-year-old adolescents among African American families. Second, it aims to examine the correlation between PSs and FPPs and frequency of family meals. A total of 211 parent–adolescent dyads completed an online survey using Qualtrics. Four PSs (i.e., authoritative, authoritarian, setting rules/expectations, and neglecting) and four FPPs (i.e., monitoring, reasoning, copying, and modeling) were identified for this study, along with family meal frequency. Body mass index (BMI) percentile and BMI were used to assess the obesity status of the adolescents and parents, respectively. No correlation was found between the adolescents’ and parents’ obesity status and the PSs and FPPs, while the adolescents’ BMI percentile was significantly correlated with parental BMI. However, a higher number of family meals decreased the likelihood of obesity among the adolescents to some extend and depended on the type of BMI used. An authoritative PS was the only style related to family meal frequency, while three FPPs, namely, monitoring, reasoning, and modeling, were related to a greater number of family meals in African American families. The findings of this study can be used in the development of parental education workshops/sessions, with consideration of the cultural differences in African American families, and can help parents to adopt the best parenting strategy to promote the healthy weight status of their adolescents.
Singing Interventions in Pulmonary Rehabilitation: A Scoping Review
Authors: Kim Soo Ji , Yeo Myung Sun , Kim So Yeon
Published: January 2023

(1) Background: Individuals with pulmonary disease need intensive and consistent rehabilitation due to their high risk for serious illness and long-term complications. The purpose of this scoping review was to provide a comprehensive analysis of relevant research regarding the use of singing in pulmonary rehabilitation. (2) Methods: A systematic literature search was performed using the PsycINFO, CINAHL, PubMed, and Web of Science databases. A search for studies that employed singing in pulmonary rehabilitation for patients with pulmonary disease was conducted. (3) Results: Studies that met the selection criteria were summarized and analyzed. Twenty-seven studies were included in the final analysis. Results showed that research using singing in pulmonary rehabilitation generally employed an intervention with structured tasks and additional home practice or socialization time. However, the singing procedure in each intervention was not always specifically described and the findings were inconsistent. (4) Conclusions: Programmed singing interventions can support lung health and be an effective component of pulmonary rehabilitation. The therapeutic singing method in relation to respiratory exercises should be integrated into the main activity in the intervention. Overall, singing has physical and psychosocial effects, leading to improvements in symptoms, but more research is necessary to ensure that the respiratory needs of people with pulmonary disease are adequately met.
Relapse among MHCUs after a Short-Term Admission in an Acute Psychiatric Unit: Primary Caregivers’ Perspective
Authors: Raluthaga Nelson , Shilubane Hilda N. , Lowane Mygirl Pearl
Published: January 2023

South Africa has taken initiative to strengthen its mental health system, by improving the Mental Health Care Act 17 of 2002 which proclaims that mental healthcare users (MHCUs) can be treated in communities and homes. Due to short-term hospitalisations for acute MHCUs and advocacy for community-based care, families play a significant role in providing care to severe mental healthcare users. The objective of the study was to explore primary caregivers’ perspective regarding the relapse of MHCUs following a short-term admission in acute psychiatric units. A qualitative explorative design was used. In-depth individual interviews were conducted with 18 primary caregivers whose family members were readmitted to four hospitals with units designated for acute MHCUs in Limpopo. NVivo computer software version 11 was used to analyse data. The findings are that MHCUs deny the mental health condition. Mental illness is considered a short illness that can be cured, which shows misconceptions about self-mental health conditions. Refusal of direct observed treatment support also emerged; hence, it is difficult for caregivers to identify if the patient is taking the correct doses or not taking the medication at all. Perceived wrong beliefs about mental illness can affect the patient’s desire to seek proper management and it can be damaging in many ways. Drugs and alcohol abuse makes MHCUs display disruptive behaviours and contribute to treatment non-adherence resulting in caregivers becoming reluctant to be around them. In conclusion, mixing traditional and faith-based mental healthcare practices as reported by primary caregivers can mean that tailor-fabricated culture-specific mental healthcare is required.
Comparing Mobility-Based PM2.5 Concentrations and Activity Satisfaction in Beijing between 2012 and 2017
Authors: Rao Jingwen , Ma Jing , Chai Yanwei
Published: January 2023

Although the negative effect of air pollution on life satisfaction has been examined in many studies, comparative analyses of mobility-based exposures to air pollution and momentary wellbeing have been rare to date, despite the fact that they are essential to improve wellbeing. Drawing on individuals’ space–time trajectories of two surveys conducted in 2012 and 2017 in Beijing, we investigate the temporal variations in activity satisfaction and mobility-based air pollution from monitoring stations and real-time air pollutant sensors, respectively. Furthermore, we explore how mobility-based air pollution dynamically influences activity satisfaction. The results show that air quality in Beijing improved from 2012 to 2017, and activity satisfaction increased as well. The negative relationship between them is more significant on workdays but insignificant on weekends. Moreover, real-time air pollution data show higher accuracy than monitor-based data, which suggests that future studies should pay more attention to real-time air pollution assessments.
Relationship of Motor Impairment with Cognitive and Emotional Alterations in Patients with Multiple Sclerosis
Authors: Cuerda-Ballester María , Martínez-Rubio David , García-Pardo María , Proaño Belén , Cubero Laura , Calvo-Capilla Antonio , Sancho-Cantus David , de la Rubia Ortí Jose
Published: January 2023

Introduction. Multiple sclerosis (MS) is a neurodegenerative disease that, despite mainly affecting women, is more severe in men and causes motor, cognitive and emotional alterations. The objective of this study was to determine the possible relationship between motor, cognitive and emotional alterations. Materials and Methods. This is a descriptive, observational and cross-sectional study, with 67 patients with MS (20 men and 47 women), who were given the following questionnaires: Expanded Disability Status Scale (EDSS), Two-Minute Walk Test (2MWT), Berg Balance Scale, Beck’s Depression Inventory (BDI-II), State-Trait Anxiety Inventory (STAI) and Prefrontal Symptoms Inventory (PSI) to analyze their cognitive level, body mass index (BMI) and percentage of muscle mass. In addition, regression analysis was conducted to study the relationship among variables. Results. No significant differences were found between men and women in any of the variables. Regarding the relationship between parameters, the regression analysis was statistically significant, showing an effect of age on the walking and balance performance (β ≅ −0.4, p < 0.05); in addition, there was a relationship between 2MWT and STAI A/S, indicating that both older age and a high anxiety state could impact walking performance. On the other hand, prefrontal symptoms showed moderate relationships with both anxiety and depression (β ≅ 0.6, p < 0.05); thus, high levels of anxiety and depression could increase prefrontal alterations. Conclusions. There is a relationship between motor and emotional variables. Specifically, state anxiety is related to walking resistance. No relationship was found between depression and cognitive alteration and balance or walking ability. Only age has an effect in these relationships.
Exploring the Determinants of Polypharmacy Prescribing and Dispensing Behaviors in Primary Care for the Elderly—Qualitative Study
Authors: Taghy Najwa , Ramel Viviane , Rivadeneyra Ana , Carrouel Florence , Cambon Linda , Dussart Claude
Published: January 2023

Polypharmacy is particularly prevalent in the elderly. The interest in this issue is growing, and many interventions exist to improve the appropriate use of polypharmacy for older people. However, evidence of their effectiveness is still limited. Thus, the aim of this study, based on a qualitative approach, was to identify the key elements perceived to influence the prescribing and dispensing of appropriate polypharmacy to older people in primary care. Semistructured interviews were conducted with general practitioners and community pharmacists practicing in the region of Nouvelle-Aquitaine (France). Pre-existing topic guides based on the 12 TDF domains have been adapted and used. Data were analyzed using the framework method and content analysis. A focus group of healthcare professionals was conducted, and behavior change techniques (BCTs) were used to select the intervention components. Seventeen interviews were convened. A wide range of determinants were identified as barriers and/or facilitators. Nine domains were selected as key domains to target for intervention. Five intervention components (behavior change techniques—BCTs) to include in an intervention were finally selected. The results of this study will serve as a starting point for the design of a theory-based intervention targeting healthcare professionals to improve appropriate prescribing and dispensing of polypharmacy for older people in primary care.
Predicting Older Adults’ Mobile Payment Adoption: An Extended TAM Model
Authors: Yang Cheng-Chia , Yang Shang-Yu , Chang Yu-Chia
Published: January 2023

This study adopted an advanced model, combining the technology acceptance model, the theory of reasoned action, the diffusion of innovations, trust, and five aspects of perceived risk, to measure the factors that influence the behavioral intentions of older adults to use mobile payments. A total of 365 questionnaires were collected from older adults aged 55 years or older from 20 community care sites in central Taiwan. Partial least-squares structural equation modeling was used to test our research model. The results showed that attitude was the main determinant of M-payment in older adults. Moreover, increasing the usefulness, ease of use, and observability of M-payment helped older adults improve their attitudes toward M-payment, thereby increasing their intention to use it. Trust had a significant effect on the usefulness and ease of use of M-payment, while the main factors affecting trust were only performance and financial risks.
Study on Water Resource Carrying Capacity of Zhengzhou City Based on DPSIR Model
Authors: Jia Yizhen , Wang Han
Published: January 2023

Based on the driving force–pressure–state–impact–response (DPSIR) model, a comprehensive evaluation index system is constructed. The index weight is determined by the combination weighting method in combination with the data of 2010–2019. The TOPSIS model is used to comprehensively analyze the water resource carrying capacity of Zhengzhou as the central city in China with a developed economy and relatively short water resources. The study results are as follows. (1) During the sample period, the comprehensive evaluation value of water resources carrying capacity of Zhengzhou increases from 0.4183 in 2010 to 0.5560 in 2019, with an overall fluctuating rise. Simultaneously, the water resource carrying capacity grade improves from Grade III (normal carrying capacity) to Grade II (good carrying capacity). (2) The contribution of each subsystem to the comprehensive evaluation value increases year by year. Among them, S subsystem and I subsystem make the largest contribution to the comprehensive carrying capacity. R subsystem makes a relatively stable contribution to the overall carrying capacity. Affected by GDP growth rate and uneven temporal–spatial distribution of water resources in Zhengzhou, the D subsystem and P subsystem of water resource carrying capacities show the fluctuating change. Finally, based on the above conclusions, this paper puts forward the countermeasures and suggestions to improve the level of water resource carrying capacity of Zhengzhou.
Can Regional Integration Reduce Urban Carbon Emission? An Empirical Study Based on the Yangtze River Delta, China
Authors: Yan Dongsheng , Li Pingxing
Published: January 2023

Regional integration can significantly affect carbon emissions, but scholars have paid more attention to the impact of integration level, ignoring the importance of regional integration expansion. This study attempts to demonstrate whether, in the process of promoting carbon peak and carbon neutrality in China, the transformation of the administrative region’s economy into an integrated economy based on urban agglomeration regional integration expansion affects urban carbon emissions. This study considers the regional integration expansion of the Yangtze River Delta Urban Economic Coordination Committee as a quasi-natural experiment, exploring the carbon emission reduction effect of regional integration with the difference-in-differences model. With the mediating and moderating effect models, this study examines the mechanism of regional integration affecting urban carbon emissions. The results show that regional integration, considering regional integration expansion, can significantly reduce urban carbon emissions. The carbon emission reduction effects of regional integration show significant heterogeneity. For example, there is a significant carbon emission reduction effect of high-hierarchy cities and an insignificant carbon emission reduction effect of general-hierarchy cities. Further research into the driving mechanism finds that deepening collaborative governance, industrial structure optimization, and green technology promotion brought about by regional integration are important mechanisms influencing urban carbon emissions. In addition, the carbon emission reduction effect of regional integration is influenced by the level of urban marketization and development efficiency. Different from the existing studies focusing on the effects of regional integration level, this study assesses the feasibility of promoting urban green development through urban agglomeration regional integration expansion. Based on the relevant empirical research, we propose to better promote high-quality development by strengthening urban agglomeration cooperation, optimizing urban development paths, strengthening innovative development, and improving macro political systems. It also indicates that the relevant policies should be formulated after considering local conditions.
Physical Activity Types, Physical Activity Levels and Risk of Diabetes in General Adults: The NHANES 2007–2018
Authors: Li Chunnan , Shang Shaomei , Liang Wannian
Published: January 2023

Background: Recreational activities show benefits for diabetes prevention, but work-related activity and the total amount of individual physical activity is rarely discussed. Purpose: The purpose of this study was to evaluate the participation in five typical physical activities (vigorous work activity, vigorous recreational activities, moderate work activity, moderate recreational activities, and walk/bicycle for transportation), as well as the weekly distribution of total physical activity intensity, and to explore the relationships between physical activity types, physical activity levels, and risk of diabetes. Study design: Cross-sectional study. Methods: The self-reported physical activity data on specific domains of physical activity were acquired from individuals in the 2007–2018 National Health and Nutrition Examination Survey (NHANES) using the Physical Activity Questionnaire (PAQ). Diabetes status was assessed by self-reported medical diagnosis or medication usage, or a fasting glucose concentration ≥ 126 mg/dL (fasting is defined as no caloric intake for at least 8 h) or HbA1c ≥ 6.5%. Weighted logistic regression was used to investigate the associations between physical activity types, physical activity levels, and risk of diabetes. Results: Diabetes was less prevalent in people who participated in physical activity and the risk of diabetes reduced progressively as total physical activity levels increased. Younger adults (20–44 years) and males reported a higher proportion of high-intensity physical activity participation. Conclusions: Our findings highlight the importance of a physically active lifestyle for preventing diabetes. Distinct types of physical activity had different effects on the risk of diabetes. A greater total physical activity level was related to a substantial reduction in diabetes risk.
Underage Youth Continue to Obtain E-Cigarettes from Retail Sources in 2022: Evidence from the Truth Continuous Tracking Survey
Authors: Do Elizabeth K. , Aarvig Kathleen , Donovan Emily M. , Schillo Barbara A. , Vallone Donna M. , Hair Elizabeth C.
Published: January 2023

(1) Background: This study aims to describe the primary sources of e-cigarettes among young people and to explore how these sources may differ by individual-level characteristics. (2) Methods: Data were obtained from a cross-sectional, continuous tracking survey of participants. The analytic sample includes current e-cigarette users (aged 15–20 years) surveyed from January to August 2022 (N = 1296). Respondents provided information on e-cigarette source of acquisition, device type, and flavors used, as well as sociodemographic and residential characteristics. Chi-square tests were used to determine differences in source of acquisition by age, gender, race/ethnicity, United States (US) census region, urban-rural classification, flavors used, and device type. (3) Results: Although most current e-cigarette users obtained their devices through a social source (56.9%), a considerable proportion obtained e-cigarettes from a retail source (43.1%). The primary retail sources of e-cigarette acquisition were vape shops (22.0%) and gas station/convenience stores (15.9%). Source of e-cigarette acquisition differed by age, gender, US census region, flavors used, and device type, such that a lower proportion of those who were younger, female, residing in the West, and used vape pens had reported obtaining e-cigarettes via retail sources. (4) Conclusions: Results indicate that a significant proportion of youth report obtaining e-cigarettes from retail sources, despite the federal, state, and local policies that prohibit the sale of any tobacco products to those under the age of 21. Comprehensive retail regulations to help restrict tobacco product access are needed to reduce e-cigarette use among young people.
Smart Mobility and Smart Climate: An Illustrative Case in Seville, Spain
Authors: López-Pérez María Eugenia , Reyes-García María Eugenia , López-Sanz María Eugenia
Published: January 2023

In recent years, smart city projects and initiatives have surged around the globe. Yet, a wide range of factors determine the success or failure of such initiatives and there is still a long road ahead in terms of effective governance and innovation management. In such a context, this study explores the specific case of PCT Cartuja (science and technology park in Seville, Spain)—analyzing several smart-mobility and smart-climate solutions from a Triple Helix Model standpoint. The authors tap into multiple information sources to describe the case and key implications of smart initiatives for both theory and management are discussed. This paper shows the current progress as well as the remaining challenges to illustrate how public-private partnerships and conflict can be effectively managed.
Characterization of the Structural and Mechanical Changes of the Biceps Brachii and Gastrocnemius Muscles in the Subacute and Chronic Stage after Stroke
Authors: García-Bernal María , González-García Paula , Madeleine Pascal , Casuso-Holgado María , Heredia-Rizo Alberto
Published: January 2023

The objective of this study was to characterize the changes of muscle tone, stiffness, and thickness of upper and lower limb muscles in stroke survivors. Forty patients with subacute or chronic stroke and 31 controls were included and measured using myotonometry (MyotonPRO), with multiple site assessments at muscle belly (MB) and musculotendinous (MT) locations of the biceps brachii and gastrocnemius muscles. Muscle thickness (ultrasonography) was obtained for each muscle. Upper and lower limb motor performance was evaluated with the Fugl–Meyer Assessment for Upper Extremity and the Functional Ambulance Category. Overall, muscle tone and stiffness were significantly higher at MT than at MB sites. Among stroke patients, differences between the paretic and nonparetic limb were found for the biceps brachii, with lower muscle tone, stiffness, and thickness of the paretic side (all, p < 0.05). There were weak to moderate correlations between mechanical (myotonometry) and structural (ultrasound) muscular changes, regardless of the post-stroke stage. This suggests that myotonometry and ultrasonography assess similar, although different, constructs and can be combined in the clinical setting. Their discriminative ability between the paretic and nonparetic sides and between participants with and without stroke differs depending on the muscle, the functional level, and the stroke stage.
The Impact of Cooperative Behavior between Social Organizations during the COVID-19 Pandemic Outbreak in Shanghai: A Simulation Approach
Authors: Fang Weipeng , Qin Changwei , Zhou Dan , Yin Jian , Liu Zhongmin , Guan Xianjun
Published: January 2023

In 2022, a new outbreak of the COVID-19 pandemic created considerable challenges for the Shanghai public health system. However, conventional prevention and control strategies, which only rely on formal organizations, inefficiently decrease the number of infections. Thus, a multi-organization management mode is needed for pandemic prevention. In this paper, we applied a stochastic actor-oriented model (SAOM) to analyze how these social organizations cooperate with others and further identify the mechanism that drives them to create a reliable and sustainable cooperative relationship network from the perspective of social network analysis. The model allowed us to assess the effects of the actor’s attributes, the network structure, and dynamic cooperative behavior in RSiena with longitudinal data collected from 220 participants in 19 social organizations. The results indicated that the number of cooperative relationships increased during the pandemic, from 44 to 162, which means the network between social organizations became more reliable. Furthermore, all the hypotheses set in four sub-models were significant (t-ratio < 0.1, overall max t-ratio < 0.25, and e/s > 2). Additionally, the estimated values showed that four factors played a positive role in forming the cooperative relationship network, i.e., all except the “same age group effect (−1.02)”. The results also indicated that the social organizations tend to build relationships with more active actors in the community in every time period. This paper is of great significance regarding the innovation of public health system management and the improvement of Chinese grassroots governance.
Healthcare Workers after Two Years of COVID-19: The Consequences of the Pandemic on Psychological Health and Sleep among Nurses and Physicians
Authors: Alfonsi Valentina , Scarpelli Serena , Gorgoni Maurizio , Couyoumdjian Alessandro , Rosiello Francesco , Sandroni Cinzia , Corsi Roberto , Pietrantonio Filomena , De Gennaro Luigi
Published: January 2023

COVID-19 has challenged the health workforce worldwide. In this cross-sectional study with a retrospective assessment, we explored the impact of the pandemic on mental health and sleep among a sample of Italian nurses and medical doctors. A total of 287 healthcare workers (212 nurses and 75 physicians) completed a web survey on socio-demographic, psychological, and sleep-related aspects referring to the period before the pandemic and to the present period of February to June 2022. Comparisons between nurses and physicians revealed that the former had greater distress in response to the pandemic. Consistently, the multivariate analysis of covariance showed that even if both groups were negatively impacted by the pandemic, nurses presented a greater worsening over time regarding several psychological and sleep symptoms. Furthermore, we observed that working on the frontline represented an additional risk factor for nurses. In line with previous evidence, we also found that personal experiences with COVID-19 are significant predictors of the current health status. Our results underscore the urgent need for preventive programs among healthcare operators to increase their coping skills and prevent the long-term consequences of chronic stress, especially for high-risk professionals. Specific attention should also be devoted to programs to improve sleep quality and reduce sleep-related traumatic symptoms.
Unhelmeted Riding, Drunk Riding, and Unlicensed Riding among Motorcyclists: A Population Study in Taiwan during 2011–2016
Authors: Liu Yen-Hsiu , Wiratama Bayu , Chao Chung-Jen , Wang Ming-Heng , Chen Rui-Sheng , Saleh Wafaa , Pai Chih-Wei
Published: January 2023

This study aimed to investigate the association between drunk riding, unhelmeted riding, unlicensed riding, and running-off-road (ROR) crashes. Multiple logistic regression was used to calculate the adjusted odds ratio (AOR) by using the National Taiwan Traffic Crash Dataset for 2011–2016. The results revealed that unhelmeted riding was associated with 138% (AOR = 2.38; CI (confidence interval) = 2.34–2.42) and 47% (AOR = 1.47; CI = 1.45–1.49) higher risks of drunk riding and unlicensed riding, respectively. The risk of unhelmeted riding increased with blood alcohol concentrations (BACs), and riders with the minimum BAC (0.031–0.05%) had nearly five times (AOR = 4.99; CI = 4.74–5.26) higher odds of unlicensed riding compared with those of riders with a negative BAC. Unhelmeted riding, drunk riding, and unlicensed riding were associated with 1.21 times (AOR = 1.21; CI = 1.13–1.30), 2.38 times (AOR = 2.38; CI = 2.20–2.57), and 1.13 times (AOR = 1.13; CI = 1.06–1.21) higher odds of ROR crashes, respectively. The three risky riding behaviours (i.e., unhelmeted riding, drunk riding, and unlicensed riding) were significantly related to ROR crashes. The risk of unhelmeted riding and ROR crashes increased with BACs.
Modeling Land Administration Data Dissemination Processes: A Case Study in Croatia
Authors: Križanović Josip , Roić Miodrag
Published: January 2023

Establishing land administration systems is enough of a challenge as it is, and the task of keeping the system up to date with developments in society is even more challenging. They have to serve society on a long-term basis and normally have a long-term return on investment; therefore, both the static and dynamic components of the system must be considered when designing land administration systems. The processes within land administration systems are registration and dissemination. In this study, the authors formalized and analyzed the two most common use cases of land administration data dissemination processes. The first use case depicts the dissemination of land use constraints imposed by spatial planning, whereas the second case depicts the dissemination of available utilities. The aim of this study was to examine how the land administration data dissemination processes could be optimized and improved in a standardized formal manner. From the formalized processes, certain elements, such as actors, activities, input and output data, and the timeframe, were identified and matched with existing LADM classes. The importance of institutional agreements and the need for more time-efficient and user-friendly access to the disseminated data are also discussed in the current paper.
The Gut–Vascular Barrier as a New Protagonist in Intestinal and Extraintestinal Diseases
Authors: Di Tommaso Natalia , Santopaolo Francesco , Gasbarrini Antonio , Ponziani Francesca Romana
Published: January 2023

The intestinal barrier, with its multiple layers, is the first line of defense between the outside world and the intestine. Its disruption, resulting in increased intestinal permeability, is a recognized pathogenic factor of intestinal and extra-intestinal diseases. The identification of a gut–vascular barrier (GVB), consisting of a structured endothelium below the epithelial layer, has led to new evidence on the etiology and management of diseases of the gut–liver axis and the gut–brain axis, with recent implications in oncology as well. The gut–brain axis is involved in several neuroinflammatory processes. In particular, the recent description of a choroid plexus vascular barrier regulating brain permeability under conditions of gut inflammation identifies the endothelium as a key regulator in maintaining tissue homeostasis and health.
Evolutionary Landscape of Tea Circular RNAs and Its Contribution to Chilling Tolerance of Tea Plant
Authors: Huang Jin , Wang Yanli , Yu Jie , Li Fangdong , Yi Lianghui , Li Yunze , Xie Na , Wu Qiong , Samarina Lidiia , Tong Wei , Xia Enhua
Published: January 2023

Chilling stress threatens the yield and distribution pattern of global crops, including the tea plant (Camellia sinensis), one of the most important cash crops around the world. Circular RNA (circRNA) plays roles in regulating plant growth and biotic/abiotic stress responses. Understanding the evolutionary characteristics of circRNA and its feedbacks to chilling stress in the tea plant will help to elucidate the vital roles of circRNAs. In the current report, we systematically identified 2702 high-confidence circRNAs under chilling stress in the tea plant, and interestingly found that the generation of tea plant circRNAs was associated with the length of their flanking introns. Repetitive sequences annotation and DNA methylation analysis revealed that the longer flanking introns of circRNAs present more repetitive sequences and higher methylation levels, which suggested that repeat-elements-mediated DNA methylation might promote the circRNAs biogenesis in the tea plant. We further detected 250 differentially expressed circRNAs under chilling stress, which were functionally enriched in GO terms related to cold/stress responses. Constructing a circRNA-miRNA-mRNA interaction network discovered 139 differentially expressed circRNAs harboring potential miRNA binding sites, which further identified 14 circRNAs that might contribute to tea plant chilling responses. We further characterized a key circRNA, CSS-circFAB1, which was significantly induced under chilling stress. FISH and silencing experiments revealed that CSS-circFAB1 was potentially involved in chilling tolerance of the tea plant. Our study emphasizes the importance of circRNA and its preliminary role against low-temperature stress, providing new insights for tea plant cold tolerance breeding.
Transcriptome Analysis Reveals the Defense Mechanism of Cotton against Verticillium dahliae Induced by Hypovirulent Fungus Gibellulopsis nigrescens CEF08111
Authors: Feng Zili , Wei Feng , Feng Hongjie , Zhang Yalin , Zhao Lihong , Zhou Jinglong , Xie Jiatao , Jiang Daohong , Zhu Heqin
Published: January 2023

Verticillium wilt is a kind of plant vascular disease caused by the soilborne fungus Verticillium dahliae, which severely limits cotton production. Our previous studies showed that the endophytic fungus Gibellulopsis nigrescens CEF08111 can effectively control Verticillium wilt and induce a defense response in cotton plants. However, the comprehensive molecular mechanism governing this response is not yet clear. To study the signaling mechanism induced by strain CEF08111, the transcriptome of cotton seedlings pretreated with CEF08111 was sequenced. The results revealed 249, 3559 and 33 differentially expressed genes (DEGs) at 3, 12 and 48 h post inoculation with CEF08111, respectively. At 12 h post inoculation with CEF08111, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that the DEGs were enriched mainly in the plant–pathogen interaction, mitogen-activated protein kinase (MAPK) signaling pathway-plant, and plant hormone signal transduction pathways. Gene ontology (GO) analysis revealed that these DEGs were enriched mainly in the following terms: response to external stimulus, systemic acquired resistance, kinase activity, phosphotransferase activity, xyloglucan: xyloglucosyl transferase activity, xyloglucan metabolic process, cell wall polysaccharide metabolic process and hemicellulose metabolic process. Moreover, many genes, such as calcium-dependent protein kinase (CDPK), flagellin-sensing 2 (FLS2), resistance to Pseudomonas syringae pv. maculicola 1(RPM1) and myelocytomatosis protein 2 (MYC2), that regulate crucial points in defense-related pathways were identified and may contribute to V. dahliae resistance in cotton. Seven DEGs of the pathway phenylpropanoid biosynthesis were identified by weighted gene co-expression network analysis (WGCNA), and these genes are related to lignin synthesis. The above genes were compared and analyzed, a total of 710 candidate genes that may be related to the resistance of cotton to Verticillium wilt were identified. These results provide a basis for understanding the molecular mechanism by which the biocontrol fungus CEF08111 increases the resistance of cotton to Verticillium wilt.
Cell-Free DNA Fragmentomics: The Novel Promising Biomarker
Authors: Qi Ting , Pan Min , Shi Huajuan , Wang Liangying , Bai Yunfei , Ge Qinyu
Published: January 2023

Cell-free DNA molecules are released into the plasma via apoptotic or necrotic events and active release mechanisms, which carry the genetic and epigenetic information of its origin tissues. However, cfDNA is the mixture of various cell fragments, and the efficient enrichment of cfDNA fragments with diagnostic value remains a great challenge for application in the clinical setting. Evidence from recent years shows that cfDNA fragmentomics’ characteristics differ in normal and diseased individuals without the need to distinguish the source of the cfDNA fragments, which makes it a promising novel biomarker. Moreover, cfDNA fragmentomics can identify tissue origins by inferring epigenetic information. Thus, further insights into the fragmentomics of plasma cfDNA shed light on the origin and fragmentation mechanisms of cfDNA during physiological and pathological processes in diseases and enhance our ability to take the advantage of plasma cfDNA as a molecular diagnostic tool. In this review, we focus on the cfDNA fragment characteristics and its potential application, such as fragment length, end motifs, jagged ends, preferred end coordinates, as well as nucleosome footprints, open chromatin region, and gene expression inferred by the cfDNA fragmentation pattern across the genome. Furthermore, we summarize the methods for deducing the tissue of origin by cfDNA fragmentomics.
Neuroprotective Effects of Erinacine A on an Experimental Model of Traumatic Optic Neuropathy
Authors: Hsu Chiao-Ling , Wen Yao-Tseng , Hsu Tzu-Chao , Chen Chin-Chu , Lee Li-Ya , Chen Wan-Ping , Tsai Rong-Kung
Published: January 2023

Erinacine A (EA), a natural neuroprotectant, is isolated from a Chinese herbal medicine, Hericium erinaceus. The aim of this study was to investigate the neuroprotective effects of EA in a rat model of traumatic optic neuropathy. The optic nerves (ONs) of adult male Wistar rats were crushed using a standardized method and divided into three experimental groups: phosphate-buffered saline (PBS control)-treated group, standard EA dose-treated group (2.64 mg/kg in 0.5 mL of PBS), and double EA dose-treated group (5.28 mg/kg in 0.5 mL of PBS). After ON crush, each group was fed orally every day for 14 days before being euthanized. The visual function, retinal ganglion cell (RGC) density, and RGC apoptosis were determined using flash visual-evoked potentials (fVEP) analysis, retrograde Fluoro-Gold labelling, and TdT-dUTP nick end-labelling (TUNEL) assay, respectively. Macrophage infiltration of ON was detected by immunostaining (immunohistochemistry) for ED1. The protein levels of phosphor-receptor-interacting serine/threonine-protein kinase1 (pRIP1), caspase 8 (Cas8), cleaved caspase 3 (cCas3), tumour necrosis factor (TNF)-α, tumour necrosis factor receptor1 (TNFR1), interleukin (IL)-1β, inducible nitric oxide synthase (iNOS), nuclear factor erythroid 2-related factor 2 (Nrf2), haem oxygenase-1 (HO-1), and superoxide dismutase 1 (SOD1) were evaluated by Western blotting. When comparing the standard EA dose-treated group and the double EA dose-treated group with the PBS-treated group, fVEP analysis showed that the amplitudes of P1–N2 in the standard EA dose group and the double EA dose-treated group were 1.8 and 2.4-fold, respectively, higher than that in the PBS-treated group (p < 0.05). The density of RGC in the standard EA dose-treated group and the double EA dose-treated group were 2.3 and 3.7-fold, respectively, higher than that in the PBS-treated group (p < 0.05). The TUNEL assay showed that the standard EA dose-treated group and the double EA dose-treated group had significantly reduced numbers of apoptotic RGC by 10.0 and 15.6-fold, respectively, compared with the PBS-treated group (p < 0.05). The numbers of macrophages on ON were reduced by 1.8 and 2.2-fold in the standard EA dose-treated group and the double EA dose-treated group, respectively (p < 0.01). On the retinal samples, the levels of pRIP, Cas8, cCas3, TNF-α, TNFR1, IL-1β, and iNOS were decreased, whereas those of Nrf2, HO-1, and SOD1 were increased in both EA-treated groups compared to those in the PBS-treated group (p < 0.05). EA treatment has neuroprotective effects on an experimental model of traumatic optic neuropathy by suppressing apoptosis, neuroinflammation, and oxidative stress to protect the RGCs from death as well as preserving the visual function.
Phytosulfokine-δ: A Small Peptide, but a Big Player in Symbiosis Gene Regulation
Authors: Suri Gurparsad Singh , Tiwari Manish
Published: January 2023

Nitrogen availability is one of the critical determinants of agricultural yield. Biological nitrogen fixation, such as legume–rhizobia symbiotic association, might function as a solution to fix nitrogen. Using phytosulfokine (PSK)-α sequences as a query, Yu et al., 2022 performed a comprehensive genome-wide search of legume species to identify PSK-δ, a divergent pentapeptide differing in single amino acid. Furthermore, PSK-δ exhibited nodule-specific expression with lower expression in the root, substantiating the nodule-specific temporal expression and suggesting its role in nodule development and nitrogen fixation. Additionally, in planta functional characterization in Medicago truncatula using overexpression and Tnt1-insertion mutant analysis indicated the role of PSK-δ in symbiotic nodulation. Interestingly, a similar phenotype of MtPSKδ mutant (mtpskδ) with that of wild-type control led to the hypothesis of its functional redundancy with PSK-α in nodule organogenesis. Further investigation regarding its position in the Nod-factor signaling pathway revealed the downstream function of PSK-δ in association with MtENOD11 in regulating nodule formation.