bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Nanobody-Horseradish Peroxidase Fusion Based-Competitive ELISA to Rapidly Detect Avian Corona-Virus-Infectious Bronchitis Virus Antibody in Chicken Serum
Authors: Gu Kui , Song Zengxu , Ma Peng , Liao Ziwei , Yang Ming , Zhou Changyu , Li Chao , Zhao Yu , Li Hao , Yang Xin , Lei Changwei , Wang Hongning
Published: July 2022

Avian coronavirus-infectious bronchitis virus (AvCoV-IBV) is the causative agent of infectious bronchitis (IB) that has brought great threat and economic losses to the global poultry industry. Rapid and accurate diagnostic methods are very necessary for effective disease monitoring. At the present study, we screened a novel nanobody against IBV-N protein for development of a rapid, simple, sensitive, and specific competitive ELISA for IBV antibody detection in order to enable the assessment of inoculation effect and early warning of disease infection. Using the phage display technology and bio-panning, we obtained 7 specific nanobodies fused with horseradish peroxidase (HRP) which were expressed in culture supernatant of HEK293T cells. Out of which, the nanobody of IBV-N-Nb66-vHRP has highly binding with IBV-N protein and was easily blocked by the IBV positive serums, which was finally employed as an immunoprobe for development of the competitive ELISA (cELISA). In the newly developed cELISA, we reduce the use of enzyme-conjugated secondary antibody, and the time of whole operation process is approximately 1 h. Moreover, the IBV positive serums diluted at 1:1000 can still be detected by the developed cELISA, and it has no cross reactivity with others chicken disease serums including Newcastle disease virus, Fowl adenovirus, Avian Influenza Virus, Infectious bursal disease virus and Hepatitis E virus. The cut-off value of the established cELISA was 36%, and the coefficient of variation of intra- and inter-assay were 0.55–1.65% and 2.58–6.03%, respectively. Compared with the commercial ELISA (IDEXX kit), the agreement rate of two methods was defined as 98% and the kappa value was 0.96, indicating the developed cELISA has high consistency with the commercial ELISA. Taken together, the novel cELISA for IBV antibody detection is a simple, rapid, sensitive, and specific immunoassay, which has the potential to rapidly test IBV antibody contributing to the surveillance and control of the disease.
Antidepressant Effect of Intermittent Long-Term Systemic Administration of Irisin in Mice
Authors: Pignataro Patrizia , Dicarlo Manuela , Zerlotin Roberta , Storlino Giuseppina , Oranger Angela , Sanesi Lorenzo , Lovero Roberto , Buccoliero Cinzia , Mori Giorgio , Colaianni Graziana , Colucci Silvia , Grano Maria
Published: July 2022

Depression is a psychiatric disorder increasingly diffused worldwide. Evidence suggests that irisin, a myokine secreted by contracting muscle, mediates beneficial effects on several targets, including the brain. Here, the potential antidepressant properties of long-term intermittent systemic irisin administration (100 µg/kg/weekly for 1 month) were evaluated in mice by the Tail Suspension Test (TST), Forced Swim Test (FST), and Open Field Test (OFT). Furthermore, to deepen the molecular pathways underlying irisin treatment, the expression of irisin precursor, neurotrophic/growth factors, and cytokines was analyzed. Irisin treatment significantly decreased the immobility time in the TST and FST, suggesting an antidepressant effect. Additionally, irisin seemed to display an anxiolytic-like effect increasing the time spent in the OFT arena center. These findings were probably due to the modulation of endogenous brain factors as the gene expression of some neurotrophins, such as brain-derived neurotrophic factor (BDNF) and insulin-like growth factor (IGF-1), was upregulated only in irisin-treated mouse brain. Moreover, irisin modulated the expression of some cytokines (IL-1β, IL-4, IL-6, and IL-10). To the best of our knowledge, this is the first study demonstrating that the irisin antidepressant effect may be observed even with a systemic administration in mice. This could pave the way toward intriguing preclinical research in humans.
Expression Patterns of Drosophila Melanogaster Glutathione Transferases
Authors: Gonis Elodie , Fraichard Stéphane , Chertemps Thomas , Hecker Arnaud , Schwartz Mathieu , Canon Francis , Neiers Fabrice
Published: July 2022

Glutathione transferases (GSTs) are ubiquitous enzymes that catalyze the conjugation of glutathione to various molecules. Among the 42 GSTs identified in Drosophila melanogaster, Delta and Epsilon are the largest classes, with 25 members. The Delta and Epsilon classes are involved in different functions, such as insecticide resistance and ecdysone biosynthesis. The insect GST number variability is due mainly to these classes. Thus, they are generally considered supports during the evolution for the adaptability of the insect species. To explore the link between Delta and Epsilon GST and their evolution, we analyzed the sequences using bioinformatic tools. Subgroups appear within the Delta and Epsilon GSTs with different levels of diversification. The diversification also appears in the sequences showing differences in the active site. Additionally, amino acids essential for structural stability or dimerization appear conserved in all GSTs. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed that the transcripts corresponding to these two classes are heterogeneously expressed within D. melanogaster. Some GSTs, such as GSTD1, are highly expressed in all tissues, suggesting their general function in detoxification. Conversely, some others, such as GSTD11 or GSTE4, are specifically expressed at a high level specifically in antennae, suggesting a potential role in olfaction.
Storage Potential of the Predatory Ladybird Cheilomenes propinqua in Relation to Temperature, Humidity, and Factitious Food
Authors: Reznik Sergey Ya. , Ovchinnikov Andrey N. , Bezman-Moseyko Olga S. , Samartsev Konstantin G. , Belyakova Natalia A.
Published: July 2022

The ability of the females of the predatory ladybird Cheilomenes propinqua to survive and to retain reproductive potential in the absence of natural food (aphids) was estimated under various hydrothermal (temperatures of 7, 12, 17, 22, and 27 °C; air humidities of 50% and 80–90%) and trophic (starved vs. fed on the frozen eggs of the grain moth Sitotroga cerealella) conditions. The post-storage reproductive potential was estimated using the mean number of eggs laid over 20 days. The experiments showed that fed females can be stored at temperatures of 15–17 °C for 20 days with a rather low (about 20%) and for up to 80 days with an acceptable (not more than 50%) loss of the reproductive potential. The successful short-term (up to 3 days) storage or transportation of fed and starved females can occur at temperatures from 12 to 27 °C. However, storage for more than 10 days at temperatures of 7 °C and lower results in the 100% mortality of both the starved and fed beetles. These data suggest that (1) in greenhouses, C. propinqua can be used for the biological control of aphids by preventing colonization, although this would require the regular supplementation of factitious food, and (2) escaped individuals would not survive the winter even in the south of the temperate zone.
Determining the Effect of Different Reproduction Factors on the Yield and Hatching of Tenebrio Molitor Eggs
Authors: Frooninckx Lotte , Berrens Siebe , Van Peer Meggie , Wuyts Ann , Broeckx Laurens , Van Miert Sabine
Published: July 2022

Whereas the production of conventional livestock can rely on years of knowledge and experience, the mealworm production industry is still in its early stages. Although the yellow mealworm Tenebrio molitor has been cultivated as feed for pets and zoo animals for quite some time, the optimization of the reproduction process has not been widely explored. For reproduction, beetles are placed in crates supplemented with a substrate to oviposit their eggs. After a specified time, the beetles are removed, and the eggs are further cultivated to develop into larvae. Factors such as oviposition duration and beetle density influence the number of produced mealworms per beetle, partly due to the cannibalistic behavior of the beetles. However, reproductive success has mostly been assessed by determining the number of offspring several weeks after oviposition. As a result, the number of eggs laid and their hatch rates are unknown. In this research, eggs are separated from the oviposition substrate, i.e., harvested. This approach allows to determine the influence of beetle density, oviposition duration, beetle age and the use of a grid during oviposition on the number of eggs produced and the egg hatching rate and timing thereof. In addition, the influence of the harvesting method on the hatching of T. molitor′s eggs was determined.
Relative Efficacy and Safety of Anti-Inflammatory Biologic Agents for Osteoarthritis: A Conventional and Network Meta-Analysis
Authors: Li Yang , Mai Yiying , Cao Peihua , Wen Xin , Fan Tianxiang , Wang Xiaoshuai , Ruan Guangfeng , Tang Su’an , Ding Changhai , Zhu Zhaohua
Published: July 2022

Previous studies have consistently revealed that both local and systemic inflammations are the key to the onset and progression of osteoarthritis (OA). Thus, anti-inflammatory biologic agents could potentially attenuate the progression of OA. We conducted this meta-analysis to examine the efficacy and safety of ant-inflammatory biologic agents among OA patients. Methods: Five databases were searched for randomized controlled trials (RCTs) comparing biologics with placebo or each other in OA patients. Data of pain, physical function, stiffness, and adverse events (AEs) were extracted for a conventional and a Bayesian network meta-analysis. Results: 15 studies with data for 1566 patients were analyzed. In the conventional meta-analysis, etanercept (SMD −0.47; 95% CI −0.89, −0.05) and infliximab (SMD −2.04; CI −2.56, −1.52) were superior to placebo for knee pain. In the network meta-analysis, infliximab was superior to all the other biologic agents in improving pain (vs. hyaluronic acid (SMD −22.95; CI −34.21, −10.43), vs. adalimumab (SMD −21.71; CI −32.65, −11.00), vs. anakinra (SMD −24.63; CI −38.79, −10.05), vs. canakinumab (SMD −32.83; CI −44.45, −20.68), vs. etanercept (SMD −18.40; CI −29.93, −5.73), vs. lutikizumab (SMD −25.11; CI −36.47, −14.78), vs. naproxen (SMD −30.16; CI −41.78, −17.38), vs. tocilizumab (SMD −24.02; CI −35.63, −11.86) and vs. placebo (SMD −25.88; CI −34.87, −16.60)). No significant differences were observed between biologics and placebo regarding physical function, stiffness, and risk of AEs. Conclusions: The findings suggest that infliximab may relieve pain more than other biological agents in OA patients. No significant differences were observed between biologics and placebo regarding physical function, stiffness, and risk of AEs. The results must be interpreted cautiously; therefore, further randomized controlled trials are warranted.
Prediction of Intracranial Infection in Patients under External Ventricular Drainage and Neurological Intensive Care: A Multicenter Retrospective Cohort Study
Authors: Fu Pengfei , Zhang Yi , Zhang Jun , Hu Jin , Sun Yirui
Published: July 2022

Objective: To generate an optimal prediction model along with identifying major contributors to intracranial infection among patients under external ventricular drainage and neurological intensive care. Methods: A retrospective cohort study was conducted among patients admitted into neurointensive care units between 1 January 2015 and 31 December 2020 who underwent external ventricular drainage due to traumatic brain injury, hydrocephalus, and nonaneurysmal spontaneous intracranial hemorrhage. Multivariate logistic regression in combination with the least absolute shrinkage and selection operator regression was applied to derive prediction models and optimize variable selections. Other machine-learning algorithms, including the support vector machine and K-nearest neighbor, were also applied to derive alternative prediction models. Five-fold cross-validation was used to train and validate each model. Model performance was assessed by calibration plots, receiver operating characteristic curves, and decision curves. A nomogram analysis was developed to explicate the weights of selected features for the optimal model. Results: Multivariate logistic regression showed the best performance among the three tested models with an area under curve of 0.846 ± 0.006. Six variables, including hemoglobin, albumin, length of operation time, American Society of Anesthesiologists grades, presence of traumatic subarachnoid hemorrhage, and a history of diabetes, were selected from 37 variable candidates as the top-weighted prediction features. The decision curve analysis showed that the nomogram could be applied clinically when the risk threshold is between 20% and 100%. Conclusions: The occurrence of external ventricular-drainage-associated intracranial infections could be predicted using optimal models and feature-selection approaches, which would be helpful for the prevention and treatment of this complication in neurointensive care units.
Evaluation of Technical Condition and Durability of Wooden Shaft Guides with Application of Non-Destructive and Semi-Destructive Testing Methods
Authors: Pasek Rafał , Jaskowska-Lemańska Justyna , Wałach Daniel , Rokita Tomasz , Kamiński Paweł
Published: July 2022

This article addresses the issue of the durability of mining shaft equipment elements. Shafts as a transport route are one of the most exploited parts of a mine. Consequently, their components are exposed to high mechanical stresses, which cause the deterioration of their mechanical properties. In the case of shafts with timber components, elements such as the shaft guides are evaluated on a purely macroscopic basis and are often unnecessarily replaced. This paper presents the possibilities for the application of non-destructive methods (ultrasound and laser scanning) and semi-destructive methods (sclerometric and drill resistance tests). The experimental results suggest that it was possible to derive correlations between penetration depth and drill resistance tests with bulk density. However, these tests were not directly correlated with flexural strength. The ultrasound studies did not indicate a significant relationship with the physical or mechanical properties. In contrast, the method of comparing the variation (wear) in the tested guides using 3D laser scanning demonstrated a high accuracy; moreover, this method is independent of factors that may affect the results of penetration depth or drill resistance measurements. The application of non-destructive and semi-destructive tests for the determination of the physical and mechanical properties of timber elements of mine shafts’ equipment may enable the detection of a defect earlier or extend the service life of elements, hence limiting the downtime of shaft operation related to the replacement of elements.
The PH Domain and C-Terminal polyD Motif of Phafin2 Exhibit a Unique Concurrence in Animals
Authors: Hasan Mahmudul , Capelluto Daniel
Published: July 2022

Phafin2, a member of the Phafin family of proteins, contributes to a plethora of cellular activities including autophagy, endosomal cargo transportation, and macropinocytosis. The PH and FYVE domains of Phafin2 play key roles in membrane binding, whereas the C-terminal poly aspartic acid (polyD) motif specifically autoinhibits the PH domain binding to the membrane phosphatidylinositol 3-phosphate (PtdIns3P). Since the Phafin2 FYVE domain also binds PtdIns3P, the role of the polyD motif remains unclear. In this study, bioinformatics tools and resources were employed to determine the concurrence of the PH-FYVE module with the polyD motif among Phafin2 and PH-, FYVE-, or polyD-containing proteins from bacteria to humans. FYVE was found to be an ancient domain of Phafin2 and is related to proteins that are present in both prokaryotes and eukaryotes. Interestingly, the polyD motif only evolved in Phafin2 and PH- or both PH-FYVE-containing proteins in animals. PolyD motifs are absent in PH domain-free FYVE-containing proteins, which usually display cellular trafficking or autophagic functions. Moreover, the prediction of the Phafin2-interacting network indicates that Phafin2 primarily cross-talks with proteins involved in autophagy, protein trafficking, and neuronal function. Taken together, the concurrence of the polyD motif with the PH domain may be associated with complex cellular functions that evolved specifically in animals.
Development of Desirable Fine Ferrite Grain Size and Random Second Phase Dual-Phase Steel Microstructures Using Composition and/or Processing Modifications
Authors: Bandi Bharath , Slater Carl , Farrugia Didier , Davis Claire
Published: July 2022

Microstructural morphology is known to have a significant impact on the mechanical properties of dual-phase steels. A fine ferrite grain size and random distribution of small second phase islands are desirable to provide superior isotropic properties compared to the banded second phase distribution that is typical for this type of steel. A rapid alloy prototyping (RAP) facility has been used to investigate three different DP 800 variants by systematically varying the compositions and/or process parameters compared to the ‘standard’ DP800 composition and processing that gives a banded microstructure. For Variant 1, the heating rate during the annealing cycle after cold rolling varied between 0.65 and 30 °C/s for the 45%, 60% and 75% cold reduction samples. It was found that a cold reduction of 75% and heating rate of 15 °C/s resulted in the microstructure that can give the best combination of strength and ductility because of the fine grain size and high martensite volume fraction. For Variant 2, the effect of changing the hot rolled (HR) microstructure (ferrite–pearlite, ferrite–bainite or martensite) on the final microstructure was investigated. Both the ferrite–50% bainite and fully martensite/bainite HR materials for all cold reductions resulted in annealed microstructures with necklace martensite morphology and finer ferrite grains compared to the ferrite–pearlite HR material, which gave a typical banded ferrite–martensite microstructure with a coarser ferrite grain size. For Variant 3, the Mn content was reduced, and increased Nb was used to achieve higher pancaking during the hot rolling stage, which refined ferrite grains in the HR condition with the same hardness. After annealing with the standard parameters only the 45% cold-reduced material produced a finer ferrite grain size than the standard material, whereas the 60% and 75% cold-reduced samples required a higher heating rate to achieve finer ferrite grain sizes due to rapid recrystallisation and growth kinetics.
Characterization of the Isothermal and Thermomechanical Fatigue Behavior of a Duplex Steel Considering the Alloy Microstructure
Authors: Schellert Steven , Müller Julian , Ohrndorf Arne , Gorr Bronslava , Butz Benjamin , Christ Hans-Jürgen
Published: July 2022

Isothermal and thermomechanical fatigue behavior of duplex stainless steel (DSS) X2CrNiMoN22-5-3 was investigated. The aim of this work was to understand the fatigue behavior by correlation of the isothermal and thermomechanical fatigue behavior with microstructural observations. Fatigue tests at plastic-strain-amplitude of 0.2% were carried out at 20, 300 and 600 °C, while in-phase (IP) and out-of-phase (OP) thermomechanical fatigue (TMF) experiments were performed between 300 and 600 °C. During the 20 °C fatigue test, a continuous softening was observed. Transmission electron microscopy examinations reveal pronounced planar slip behavior in austenite. At 300 °C, deformation concentrates in the ferrite, where strong interactions between CrxN and dislocations were observed that explain the pronounced cyclic hardening. DSS studied exhibits softening throughout the whole isothermal fatigue test at 600 °C. In ferrite, during the 600 °C fatigue test, the G phase, γ′ austenite precipitated, and an unordered dislocation arrangement was observed. The stress responses of the TMF tests can be correlated to those of the isothermal fatigue tests. In IP mode, a positive mean stress resulted in premature failure. No γ′ austenite but the formation of subgrains in the ferrite phase was observed after TMF tests. The plastic deformation of the austenite at high temperatures results in an unordered dislocation arrangement.
A Superior High-Strength Dilute Mg-Bi-Ca Extrusion Alloy with a Bimodal Microstructure
Authors: Meng Shuaiju , Zhang Mingchi , Xiao Haoran , Luo Zhanju , Yu Wei , Jiang Runlin , Cheng Xueqi , Wang Lidong
Published: July 2022

Improving the mechanical properties of Mg alloys is of great significance for their wide application. A micro-alloyed Mg-1.45Bi-0.79Ca alloy (in wt.%) exhibiting a high tensile yield strength of 394 ± 5 MPa and a moderate elongation of 6.6 ± 0.6% was fabricated by single pass extrusion. The superior high strength is mainly attributed to the synergy effects of ultra-fine dynamic recrystallized grains; numerous Mg2Ca, Mg3Bi2, and Mg2Bi2Ca nano-precipitates; residual dislocations; sub-grain boundaries; as well as strong <10-10> fibre texture in the extrusion direction.
Bond Wire Damage Detection Method on Discrete MOSFETs Based on Two-Port Network Measurement
Authors: Yun Minghui , Cai Miao , Yang Daoguo , Yang Yiren , Xiao Jing , Zhang Guoqi
Published: July 2022

Bond wire damage is one of the most common failure modes of metal-oxide semiconductor field-effect transistor (MOSFET) power devices in wire-welded packaging. This paper proposes a novel bond wire damage detection approach based on two-port network measurement by identifying the MOSFET source parasitic inductance (LS). Numerical calculation shows that the number of bond wire liftoffs will change the LS, which can be used as an effective bond wire damage precursor. Considering a power MOSFET as a two-port network, LS is accurately extracted from frequency domain impedance (Z−parameter) using a vector network analyzer under zero biasing conditions. Bond wire cutoff experiments are employed to validate the proposed approach for bond wire damage detection. The result shows that LS increases with the rising severity of bond wire faults, and even the slight fault shows a high sensitivity, which can be effectively used to quantify the number of bond wire liftoffs of discrete MOSFETs. Meanwhile, the source parasitic resistance (RS) extracted from the proposed two-port network measurement can be used for the bond wire damage detection of high switching frequency silicon carbide MOSFETs. This approach offers an effective quality screening technology for discrete MOSFETs without power on treatment.
Bio-Inspired Hierarchical Micro-/Nanostructures for Anti-Icing Solely Fabricated by Metal-Assisted Chemical Etching
Authors: Zhang Lansheng , Chu Xiaoyang , Tian Feng , Xu Yang , Hu Huan
Published: July 2022

We report a cost-effective and scalable methodology for producing a hierarchical micro-/nanostructured silicon surface solely by metal-assisted chemical etching. It involves two major processing steps of fabricating micropillars and nanowires separately. The process of producing micro-scale structures by masked metal-assisted chemical etching was optimized. Silicon nanowires were created on the micropillar’s surface via maskless metal-assisted chemical etching. The hierarchical micro-/nanostructured surface exhibits superhydrophobic properties with a high contact angle of ~156° and a low sliding angle of <2.5° for deionized water. Furthermore, due to the existence of microscale and nanoscale air trapped at the liquid/solid interface, it exhibits a long ice delay time of 2876 s at −5 °C, more than 5 times longer than that of smooth surfaces. Compared to conventional dry etching methods, the metal-assisted chemical etching approach excludes vacuum environments and high-temperature processes and can be applied for applications requiring hierarchical micro-/nanostructured surfaces or structures.
Study of the Influence of Cutting Edge on Micro Cutting of Hardened Steel Using FE and SPH Modeling
Authors: Chaabani Lobna , Piquard Romain , Abnay Radouane , Fontaine Michaël , Gilbin Alexandre , Picart Philippe , Thibaud Sébastien , D’Acunto Alain , Dudzinski Daniel
Published: July 2022

Micromachining allows the production of micro-components with complex geometries in various materials. However, it presents several scientific issues due to scale reduction compared to conventional machining. These issues are called size effects. At this level, micromachining experiments raise technical difficulties and significant costs. In this context, numerical modeling is widely used in order to study these different size effects. This article presents four different numerical models of micro-cutting of hardened steel, a Smooth Particle Hydrodynamics (SPH) model and three finite element (FE) models using three different formulations: Lagrangian, Arbitrary Eulerian–Lagrangian (ALE) and Coupled Eulerian–Lagrangian (CEL). The objective is to study the effect of tool edge radius on the micro-cutting process through the evolution of cutting forces, chip morphology and stress distribution in different areas and to compare the relevance of the different models. First, results obtained from two models using FE (Lagrangian) and SPH method were compared with experimental data obtained in previous work. It shows that the different numerical methods are relevant for studying geometrical size effects because cutting force and stress distribution correlate with experimental data. However, they present limits due to the calculation approaches. For a second time, this paper presents a comparison between the four different numerical models cited previously in order to choose which method of modeling can present the micro-cutting process.
Geochemistry, U-Pb Zircon Ages and Hf Isotopes of Basement Rocks Beneath the Northeastern Margin of the Ordos Basin: Constraints on the Paleoproterozoic Evolution of the Western North China Craton
Authors: Xing En Yuan , Zhang Yong Sheng , Zheng Mian Ping , Wu Su Juan , Gui Bao Ling , Peng Yuan
Published: July 2022

SHRIMP zircon ages, Hf-in-zircon isotopic compositions and whole rock geochemistry were analyzed on basement metamorphic rocks from drill cores collected from the northeastern margin of the Ordos Basin. Geochemical data from four metasedimentary rocks show large variations in major element compositions, but have similar REE patterns and trace element compositions, with ΣREE = 161.80 × 10−6~341.82 × 10−6, δEu = 0.26~0.63 and LaN/YbN = 3.44~25.38. SHRIMP zircon U-Pb dating of granitic gneiss yielded the magmatic zircon ages between 1856 ± 14 Ma and 2188 ± 11 Ma, with the upper intercept age of 2229 ± 88 Ma. The cores have εHf(t) values of −5.46 to +6.57, and Hf model ages vary from 2869 Ma to 2300 Ma. The analyses of metamorphic overgrowths on zircon grains yield an average metamorphic age of 1865 ± 17 Ma (MSWD = 3.5). The zircon cores have εHf(t) values of −3.98 to 1.95, and Hf model ages vary from 2782 to 2416 Ma. Combined with data from earlier studies, we draw the conclusion that the metamorphic rocks in the borehole were formed during middle Paleoproterozoic time and were involved in a major late Paleoproterozoic tectono-thermal event which might be attributable to the collision between the Western and Eastern Block along the TNCO (the Trans-North China Orogen). The basement rocks are petrologically, geochronologically and geochemically comparable with rocks from the Khondalite series. The fact that the material properties of basement rocks beneath the northeastern Ordos Basin are different from those of the Western Block indicates that the extent and exact boundaries of the Ordos Block need to be refined.
The Molten Globule State of a Globular Protein in a Cell Is More or Less Frequent Case Rather than an Exception
Authors: Bychkova Valentina E. , Dolgikh Dmitry A. , Balobanov Vitalii A. , Finkelstein Alexei V.
Published: July 2022

Quite a long time ago, Oleg B. Ptitsyn put forward a hypothesis about the possible functional significance of the molten globule (MG) state for the functioning of proteins. MG is an intermediate between the unfolded and the native state of a protein. Its experimental detection and investigation in a cell are extremely difficult. In the last decades, intensive studies have demonstrated that the MG-like state of some globular proteins arises from either their modifications or interactions with protein partners or other cell components. This review summarizes such reports. In many cases, MG was evidenced to be functionally important. Thus, the MG state is quite common for functional cellular proteins. This supports Ptitsyn’s hypothesis that some globular proteins may switch between two active states, rigid (N) and soft (MG), to work in solution or interact with partners.
Novel C60 Fullerenol-Gentamicin Conjugate–Physicochemical Characterization and Evaluation of Antibacterial and Cytotoxic Properties
Authors: Nurzynska Aleksandra , Piotrowski Piotr , Klimek Katarzyna , Król Julia , Kaim Andrzej , Ginalska Grazyna
Published: July 2022

This study aimed to develop, characterize, and evaluate antibacterial and cytotoxic properties of novel fullerene derivative composed of C60 fullerenol and standard aminoglycoside antibiotic–gentamicin (C60 fullerenol-gentamicin conjugate). The successful introduction of gentamicin to fullerenol was confirmed by X-ray photoelectron spectroscopy which together with thermogravimetric and spectroscopic analysis revealing the formula of the composition as C60(OH)12(GLYMO)11(Gentamicin)0.8. The dynamic light scattering (DLS) revealed that conjugate possessed ability to form agglomerates in water (size around 115 nm), while Zeta potential measurements demonstrated that such agglomerates possessed neutral character. In vitro biological assays indicated that obtained C60 fullerenol-gentamicin conjugate possessed the same antibacterial activity as standard gentamicin against Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Escherichia coli, which proves that combination of fullerenol with gentamicin does not cause the loss of antibacterial activity of antibiotic. Moreover, cytotoxicity assessment demonstrated that obtained fullerenol-gentamicin derivative did not decrease viability of normal human fibroblasts (model eukaryotic cells) compared to control fibroblasts. Thus, taking into account all of the results, it can be stated that this research presents effective method to fabricate C60 fullerenol-gentamicin conjugate and proves that such derivative possesses desired antibacterial properties without unfavorable cytotoxic effects towards eukaryotic cells in vitro. These promising preliminary results indicate that obtained C60 fullerenol-gentamicin conjugate could have biomedical potential. It may be presumed that obtained fullerenol may be used as an effective carrier for antibiotic, and developed fullerenol-gentamicin conjugate may be apply locally (i.e., at the wound site). Moreover, in future we will evaluate possibility of its applications in inter alia tissue engineering, namely as a component of wound dressings and implantable biomaterials.
Research Progress on the Preparation and Applications of Laser-Induced Graphene Technology
Authors: Guo Yani , Zhang Cheng , Chen Ye , Nie Zhengwei
Published: July 2022

Graphene has been regarded as a potential application material in the field of new energy conversion and storage because of its unique two-dimensional structure and excellent physical and chemical properties. However, traditional graphene preparation methods are complicated in-process and difficult to form patterned structures. In recent years, laser-induced graphene (LIG) technology has received a large amount of attention from scholars and has a wide range of applications in supercapacitors, batteries, sensors, air filters, water treatment, etc. In this paper, we summarized a variety of preparation methods for graphene. The effects of laser processing parameters, laser type, precursor materials, and process atmosphere on the properties of the prepared LIG were reviewed. Then, two strategies for large-scale production of LIG were briefly described. We also discussed the wide applications of LIG in the fields of signal sensing, environmental protection, and energy storage. Finally, we briefly outlined the future trends of this research direction.
The Effect of Curcumin on Renal Ischemia/Reperfusion Injury in Diabetic Rats
Authors: Machado Douglas Ikedo , de Oliveira Silva Eloiza , Ventura Sara , Vattimo Maria de Fatima Fernandes
Published: July 2022

Chronic kidney disease (CKD) and acute kidney injury (AKI) are global health problems that affect over 850 million people, twice the number of diabetic individuals around the world. Diabetes mellitus (DM) is known to increase the susceptibility to AKI. Plants and foods, such as curcumin, are traditionally used as treatments for various diseases due to its wide range of bioactive compounds that exert antioxidative, anti-inflammatory, antimicrobial and anticancer properties. The aim of this study is to evaluate the effect of curcumin in diabetic rats with AKI. Adult male Wistar rats, weighing between 250 and 290 g, were randomized into four groups: Citrate (citrate buffer, i.v., single dose, on Day 1 of the protocol); DM (streptozotocin (STZ), 65 mg/k, single dose, i.v., on Day 1); DM + I/R (DM rats that, on Day 26, had the renal pedicle clamped for 30 min on both sides); DM + I/R + Curcumin (DM + I/R rats submitted to curcumin treatment). Results showed that IR worsened renal function and oxidative stress in DM rats, but the DM + IR + Curcumin group showed an increase in inulin clearance and a decrease in serum creatinine and in NGAL, in addition to an improvement in renal hemodynamics. These effects were accompanied by a reduction in oxidative and nitrosative metabolites and an increase in the thiol antioxidant reserve when curcumin was administered to the DM + IR group.
No Difference in Tolerance between Wheat and Spelt Bread in Patients with Suspected Non-Celiac Wheat Sensitivity
Authors: Zimmermann Julia , Longin Friedrich H. , Schweinlin Anna , Basrai Maryam , Bischoff Stephan C.
Published: July 2022

Individuals with suspected non-celiac wheat sensitivity (NCWS) often report better tolerance of spelt (Triticum aestivum ssp. spelta) compared to wheat (Triticum aestivum ssp. aestivum) bakery products. This experience has neither been validated nor explained on a molecular level. Therefore, we performed blinded wheat and spelt bread challenge in this patient group. Twenty-four adults with a history of NCWS but suspected spelt tolerance were challenged in a single-blinded crossover design over six weeks with six different study breads each at 300 g per day for 4 days followed by a washout phase of 3 days. Study breads comprised spelt and wheat breads made either after a traditional (T) or a current (C) recipe, resulting in four bread types plus a gluten-free bread with 1.5% added oligosaccharides (+FODMAP) and a gluten-free bread with 5% added wheat gluten (+Gluten). The main outcome parameter was the Irritable Bowel Syndrome—Severity Scoring System, which was higher than self-estimated by the participants after spelt bread consumption (p = 0.002 for T; p = 0.028 for C) and lower for wheat bread (p = 0.052 for T; p = 0.007 for C), resulting in no difference between wheat and spelt bread tolerance. The +FODMAP bread was better tolerated than both T breads (p = 0.003 for spelt; p = 0.068 for wheat) and equally well tolerated as both C breads and +Gluten breads after normalization to the washout scores. Neither signs of inflammation nor markers for intestinal barrier integrity were influenced. Our data do not confirm, on an objective basis, the differences in expected symptoms resulting from wheat and spelt products, suggesting a strong nocebo effect for wheat and a placebo effect for spelt.
Research Quality—Lessons from the UK Research Excellence Framework (REF) 2021
Authors: Thompson David R. , McKenna Hugh P.
Published: July 2022

Research quality is a term often bandied around but rarely clearly defined or measured [...]
Trichurosis on a Conventional Swine Fattening Farm with Extensive Husbandry—A Case Report
Authors: Bünger Moritz , Renzhammer René , Joachim Anja , Hinney Barbara , Brunthaler René , Al Hossan Mohamad , Matt Julia , Nedorost Nora , Weissenbacher-Lang Christiane , Schwarz Lukas
Published: July 2022

Helminth infections of swine regain clinical and economic importance due to the increasing demand for pork from extensive husbandry. Infections with Trichuris suis in pigs can lead to wasting and diarrhoea. This was demonstrated by a case of clinical trichurosis on a conventional fattening farm, where pigs were kept on pasture. While all pre-fattening pigs, which had not been on the pasture yet, had a good body condition and firm faeces, diarrhoea and poor body condition were observed in approximately half of the fattening pigs kept on pasture. Rectally collected faecal samples from all animals were investigated using faecal flotation. High numbers of T. suis eggs were detected in 17 out of 32 faecal samples, while all samples from pre-fattening pigs were negative. The highest number of eggs per gram of faeces was 778,000. Two out of three environmental samples were also positive for T. suis in faecal flotation. This case demonstrates that T. suis must be considered as an enteropathogen in pigs kept on pasture, as favourable environmental conditions, and the lack of removal of faeces from a pasture can lead to the accumulation of large numbers of infective eggs in the pigs’ surroundings.
Genome Analysis of ESBL-Producing Escherichia coli Isolated from Pigs
Authors: Founou Luria Leslie , Founou Raspail Carrel , Allam Mushal , Ismail Arshad , Essack Sabiha Yusuf
Published: July 2022

The resistome, virulome and mobilome of extended spectrum ß-lactamase (ESBL)-producing Escherichia coli (ESBL-Ec) isolated from pigs in Cameroon and South Africa were assessed using whole genome sequencing (WGS). Eleven clonally related phenotypic ESBL-Ec isolates were subjected to WGS. The prediction of antibiotic resistance genes, virulence factors (VFs) and plasmids was performed using ResFinder, VirulenceFinder and PlasmidFinder, respectively. Diverse sequence types (STs) were detected with ST2144 and ST88 being predominant and blaCTX-M-15 (55%) being the principal ESBL gene. All except two isolates harboured various aminoglycoside resistance genes, including aph(3″)-Ib (6/11, 55%) and aph(6)-1d (6/11, 55%), while the qnrS1 gene was identified in four of the isolates. The ESBL-Ec isolates showed a 93.6% score of being human pathogens. The fim, ehaB, ibeB/C were the leading virulence factors detected. All isolates harboured at least three extraintestinal pathogenic E. coli (ExPEC) VFs, with one isolate harbouring up to 18 ExPEC VFs. Five isolates (45.45%) harboured the plasmid incompatibility group IncF (FII, FIB, FIC, FIA). The study revealed that there is an urgent need to implement effective strategies to contain the dissemination of resistant and virulent ESBL-Ec through the food chain in Cameroon and South Africa.
Adiponectin System (Rescue Hormone): The Missing Link between Metabolic and Cardiovascular Diseases
Authors: Aljafary Meneerah Abdulrahman , Al-Suhaimi Ebtesam Abdullah
Published: July 2022

The adipose tissue, regardless of its role in generating and storing energy, acts as a key player as an endocrine tissue, producing a wide scale of cytokines/hormones called adipokines. Adipokines such as leptin, resistin, visfatin and osteopontin own pro-inflammatory effects on the cardiovascular system in some cases. In contrast, some adipokines have cardioprotective and anti-inflammatory impacts including adiponectin, omentin, and apelin. One of the key adipokines is adiponectin, the abundant peptide regulating hormone that is released mainly by adipocytes and cardiomyocytes as well as by endothelial and skeletal cells. It acts through two main receptors: AdipoR1 and AdipoR2, forming the “Adiponectin system” which effectively exerts its cellular mechanisms and responses in target cells. It regulates various metabolic processes, while adiponectin is the adipocyte hormone known for its cardioprotective impact in clinical and experimental research. It is also a well-effector metabolic adipokine, since weight loss or diet restriction show a link with rises in adiponectin concentrations, which is accompanied with increasing insulin sensitivity, glucose, and lipids-regulation via adiponectin’s antioxidant, anti-inflammatory, anti-fibrotic actions. The high adiponectin level made it an attractive player in developing therapeutical treatments for metabolic syndromes and cardiovascular disease. The elevated plasma levels of adiponectin are mostly attributed to its benefits on cardio-metabolism. In some cases, adiponectin has been paradoxically accompanied with elevated risk of cardiovascular disease, so higher adiponectin concentration is a marker of poor prediction. Thus, the adiponectin system is attractive to researchers as a biomarker of heart disease advancement and a predictor of prognosis during the term of some cardiovascular diseases and its mechanical functions in Hypertension and diabetic patients. This review highlights the physiological roles of adiponectin as an anti-inflammatory and cardioprotective hormone as well as how it plays as a biomarker and potential therapeutic tool in the cardiovascular system in adult, children, and adolescents. The adiponectin system may be seen as a rescue hormone aiding in remodeling of the cardiovascular system on both cellular and molecular levels. The paradox role of adiponectin relevant to cardiovascular mortality should be taken into consideration.
The Anatomical Differences and Physiological Responses of Sunburned Satsuma Mandarin (Citrus unshiu Marc.) Fruits
Authors: Kim Misun , Park Yosup , Yun Seok Kyu , Kim Sang Suk , Joa Jaeho , Moon Young-Eel , Do Gyung-Ran
Published: July 2022

Sunburn causes fruit browning and other physiological symptoms, reducing fruit production and quality. Therefore, we aimed to investigate the anatomical differences and abiotic stress responses in ‘Nichinan 1 gou’ satsuma mandarin (Citrus unshiu Marc.) according to the severity of sunburn damage (five grades: control, no sunburn; I to IV, increasing severity of sunburn). Additionally, the quality of sunburned and non-sunburned fruits was compared, and the sunburn-inducing temperature was estimated. Anatomical observations confirmed that with increased severity of symptoms, the damage to fruit rind surface and oil glands was increased. In the analysis of peel pigments, chlorophyll content in the rind gradually decreased compared with IV, whereas the carotenoid content gradually increased up to III. The flavonoid content in the peel and pulp was the highest in III. In the 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical analyses, the IC50 (the concentration of compound at which the percentage of inhibition is 50%) value was the lowest in grade III in peel or IV in pulp, indicating a high free radical scavenging ability. The fruit quality analysis between sunburned and non-sunburned fruits showed differences in total soluble solid content, total acidity, firmness, coloration, and free sugar and organic acid contents, indicating a significant effect on fruit quality. In the heat tolerance tests on fruit rind in the laboratory and field, the damage was confirmed at temperatures above 47 °C.
VENμS: Mission Characteristics, Final Evaluation of the First Phase and Data Production
Authors: Dick Arthur , Raynaud Jean-Louis , Rolland Amandine , Pelou Sophie , Coustance Sophie , Dedieu Gérard , Hagolle Olivier , Burochin Jean-Pascal , Binet Renaud , Moreau Agathe
Published: July 2022

VENμS (Vegetation and Environment New micro (μ) Satellite) is a micro satellite launched in 2017 by the Israeli Space Agency (ISA) and the French Centre National d’Etudes Spatiales (CNES). VENμS is a research satellite containing two very different devices: an electric Hall effect thruster and a multispectral optical camera. This paper focuses on the multispectral camera. The camera provides images at a resolution of 5 m, with a field of view of 27 km, and the orbit of the satellite was chosen to allow us to revisit of each observed site with constant angles every second day. In November 2020, VENμS ended the first phase of its mission. This phase, called VM01, allowed us to provide about 150 accurate time series over selected scientific sites over almost three years. Extensive work was conducted to calibrate the camera and assess the quality of the products. Not everything worked as planned before launch and a large amount of work was necessary to correct some defects of the camera or to improve the geometric registration of images. This article establishes the image quality VM01 final assessment including the presentation of radiometric and geometric calibration methods, the estimation of instrument performances and their associated temporal stabilities and the monitoring activities. In addition, it highlights the whole mechanism of data programming, reception and production. The end of VM01 phase is not the end of the VENμS mission, and a new phase started on a one-day repeat orbit.
IoT-Based Microclimate and Vibration Monitoring of a Painted Canvas on a Wooden Support in the Monastero of Santa Caterina (Palermo, Italy)
Authors: Trigona Carlo , Costa Eliana , Politi Giuseppe , Gueli Anna M.
Published: July 2022

The main objective of this work is the characterization and observation of the performance of an IoT measurement and monitoring system in the field of cultural heritage conservation for assessing the health condition of artworks. This article also describes the application of this system to the monitoring of a canvas painting applied on a wooden support, an artwork from the 19th century by the painter Giuseppe Patricolo depicting The Deposition, placed inside a niche in the Santa Caterina Monastery in Palermo (Italy). Considering the presence of the wooden structure, it is useful to measure not only microclimatic parameters such as temperature and humidity, but also vibrations that can in fact cause degradation phenomena in these artworks. This is a first step towards the development of mimetic systems integrated in the work of art without causing physical, mechanical or chemical alterations and ensuring that the level of microclimatic parameters is below the threshold values whose exceeding could compromise the entire artefact.
Representation Learning and Pattern Recognition in Cognitive Biometrics: A Survey
Authors: Wang Min , Yin Xuefei , Zhu Yanming , Hu Jiankun
Published: July 2022

Cognitive biometrics is an emerging branch of biometric technology. Recent research has demonstrated great potential for using cognitive biometrics in versatile applications, including biometric recognition and cognitive and emotional state recognition. There is a major need to summarize the latest developments in this field. Existing surveys have mainly focused on a small subset of cognitive biometric modalities, such as EEG and ECG. This article provides a comprehensive review of cognitive biometrics, covering all the major biosignal modalities and applications. A taxonomy is designed to structure the corresponding knowledge and guide the survey from signal acquisition and pre-processing to representation learning and pattern recognition. We provide a unified view of the methodological advances in these four aspects across various biosignals and applications, facilitating interdisciplinary research and knowledge transfer across fields. Furthermore, this article discusses open research directions in cognitive biometrics and proposes future prospects for developing reliable and secure cognitive biometric systems.