bluuebunny/crossref_metadata_2025 huggingface dataset:
Antioxidant, Anti-inflammatory, and Analgesic Activities ofCitrus reticulataBlanco Leaves Extracts: AnIn VivoandIn VitroStudy
Authors: Nasri M. , Bedjou F. , Porras D. , Martínez-Flórez S.
Published: June 2019

Citrus species are cultivated and consumed widely. Citrus have been investigated for their pharmacological activity and human health. Their beneficial effects include antibacterial, analgesic, anti-inflammatory, and antitumoral effects. This studywas designed to evaluate the analgesic effect and the antioxidant and anti-inflammatory activities ofCitrus reticulataBlanco leaves extracts (ECR) in cell and animal models. Antioxidant, anti-inflammatory, and antinociceptive activities were evaluated in mice using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical inhibition, xylene-induced ear edema, formalin assay and acetic acid-writhing response. Expression of antiinflammatory genes was measured in lipopolysaccharide (LPS)-treated Huh7 cells. ECR showed a significant DPPH radical scavenging activity. No behavioral changes or deaths were observed in mice at doses less than 2,000 mg/kg body weight. Different concentrations of methanolic and aqueous extracts (100–500 mg/kg body wt.) reduced the duration of linking behavior in the second phase of the formalin chemical nociception assay and decreased the number of acetic acidinduced writhing responses in mice, indicating significant analgesic activity. ECR also diminished xylene-induced ear swelling in mice, suggesting anIn Vivoanti-inflammatory action. No toxicity of ECR in the range of 0.1–10 μg/ml was observed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. Cell treatment with LPS-induced oxidative/ nitrosative stress as assessed by flow cytometry as the fluorescence of 2′,7′-dichlorofluorescein. This effect was significantly inhibited in a dose-dependent manner by ECR. Administration of ECR caused a dose-dependent inhibition of cytochrome P450 2E1, inducible nitric oxide synthase, tumor necrosis factor α, and interleukin-6 expression in LPS-treated cells. The present study demonstrates that extracts ofCitrus reticulataleaves are safe, having antioxidant, anti-inflammatory, and analgesic effects bothIn VivoandIn Vitro.
Résistance aux antibiotiques et mécanismes d’action des huiles essentielles contre les bactéries
Authors: Bouyahya A. , Bakri Y. , Et-Touys A. , Talbaoui A. , Khouchlaa A. , Charfi S. , Abrini J. , Dakka N.
Published: June 2019

L’augmentation de la résistance des bactéries aux antibiotiques est un problème mondial sérieux qui a orienté la recherche pour l’identification de nouvelles biomolécules avec une large activité antibactérienne. Les plantes et leurs dérivés, tels que les huiles essentielles (HE), sont souvent utilisés dans la médecine populaire. Dans la nature, les HE jouent un rôle important dans la protection des plantes. Elles contiennent une grande variété de métabolites secondaires capables d’inhiber ou de ralentir la croissance des bactéries. Les HE et leurs constituants ont des mécanismes d’action variés et très ciblés, touchant en particulier la membrane cellulaire et le cytoplasme, et dans certains cas, changeant complètement la morphologie cellulaire, voire l’expression des gènes. Dans cette brève revue, nous décrivons les mécanismes de résistance des bactéries aux antibiotiques et les modalités d’action antibactérienne des HE.
Toxicité aiguë, effets anti-inflammatoire et analgésique de l’extrait aqueux de Heinsia crinita (Afzel.) G. Taylor (Rubiaceae)
Authors: Boumba L.S. , Nsonde Ntandou G.F. , Loufoua A.B. , Makambila M.C. , Abena A.A.
Published: June 2019

Ce travail a pour but de valoriser l’utilisation traditionnelle de Heinsia crinita (Afzel.) G. Taylor (Rubiaceae), avec pour objectif d’évaluer la toxicité aiguë, les effets antiinflammatoire et analgésique de l’extrait aqueux des feuilles de Heinsia crinita (Afzel.) G. Taylor (Rubiaceae) chez les rongeurs et de réaliser un screening chimique de l’extrait aqueux des feuilles de Heinsia crinita (Rubiaceae). La méthode par classe de toxicité a permis d’évaluer la toxicité aiguë de l’extrait jusqu’à 2 000 mg/kg per os. 200 et 400 mg/ kg per os de l’extrait ont servi pour évaluer les effets sur l’oedème à la carragénine, le granulome au pellet de coton, les crampes induites par l’acide acétique et la pression mécanique de la patte. Les différents groupes chimiques ont été identifiés. L’extrait ne modifie pas le comportement général des souris, mais provoque un gain pondéral significatif à 2 000 mg/kg. Un effet anti-inflammatoire très intéressant a été mis en évidence, quasiment identique à celui du diclofénac contre l’oedème et légèrement supérieur à celui du diclofénac contre le granulome. Ces effets sont liés à la présence des flavonoïdes, des anthocyanes, des saponosides, des tanins, des stéroïdes et triterpénoïdes, des alcaloïdes et des hétérosides cardiotoniques.
Notes ethnobotanique et phytopharmacologique sur Cakile maritima Scop
Authors: Merchaoui H. , Hanana M. , Ksouri R.
Published: June 2019

Cakile maritima Scop., vraisemblablement de l’arabe kakeleh, est une plante halophyte appartenant à la famille des Brassicaceae, répandue et colonisant les sables maritimes des littoraux nord-africains, européens, asiatiques et australiens. Malgré son goût piquant et amer parfois marqué, elle peut être consommée crue ou cuite. Cette Brassicacée est connue depuis le Moyen Âge pour ses vertus médicinales et thérapeutiques variées. En effet, elle est utilisée en médecine traditionnelle pour ses propriétés diurétique, antiscorbutique, apéritive, digestive et purgative. Des études récentes ont révélé des activités biologiques nouvelles, i.e. antioxydante, antibactérienne, antifongique et molluscicide. L’acide érucique contenu dans ses graines entre dans le traitement des maladies démyélinisantes [1,2]. Les isothiocyanates, présents chez de nombreuses Brassicacées et produits d’hydrolyse des glucosinolates, sont doués de propriétés chimiopréventives et antimutagéniques. En effet, des tests biologiques ont montré que Cakile maritima possède un rôle préventif contre le cancer. Par ailleurs, la présence d’un principe actif dans les extraits de la plante présentant une propriété hydratante lui confère des utilisations en cosmétique pour des applications et soins dermatologiques. En outre, grâce à sa capacité de fixation des sols, elle pourrait jouer un rôle écologique de lutte contre l’érosion ; en agronomie, sa culture serait également envisageable en raison de son potentiel de bioremédiation, et la richesse de son huile de graine en acide érucique lui ouvre des perspectives industrielles considérables, devenant ainsi rentable économiquement.
The Sinner Dizisinin Travma Sonrası Stres Bozukluğu Bağlamında Çözümlenmesi
Authors: YILMAZ Tuğba
Published: June 2019

Bu çalışmada “The Sinner” isimli sekiz bölümden oluşan dizi klinik psikoloji bakış açısı
ile çözümlenmiştir. Dizinin başrol oyuncusu Cora bir cinayet işler ve dedektif Harry bu
cinayetin soruşturmasını yürütür. Dedektif Harry, Cora’nın travmatik bir yaşantısı
olduğundan ve bu cinayetin bu travma ile ilgili olduğundan şüphelenir. Cora’nın ailesini
ve erken dönem yaşantılarını araştırdığında Cora’nın da tam olarak hatırlayamadığı bir
dönem olduğunu öğrenir. Bu dönemi aydınlatmanın cinayeti de aydınlatacağına inanan
dedektif Harry, Cora’nın hiç istemediği olaylara maruz kaldığını ve bu olayları amnezi
nedeni ile hatırlayamadığını öğrenir. Bu bağlamda bu çalışmada çözümlenen konular
arasında psikolojik travmanın belirlenmesi ve vaka formülasyonu, aile dinamikleri ve
kardeş ilişkileri, maddenin kötüye kullanımı, travmanın psikolojik sağaltımı, hasta ve
terapist iş birliği yer almaktadır. Çözümleme yapılırken dedektif Harry psikoterapist,
Cora ise hasta olarak ele alınmıştır. Dizinin işlenen konular gereği klinik psikoloji
eğitimine nasıl katkıda bulunacağı sonuç kısmında tartışılmıştır.
Alzheimer Hastalığına Bilişsel Süreçler ve Klinik Çerçeveden Bakış: Şahsiyet Dizisinin Kritik İncelemesi
Authors: KAYNAK Hande , DENİZCİ NAZLIGÜL Merve
Published: June 2019

Şahsiyet (Saylak, 2018), İstanbul Beyoğlu’nda yaşayan Agâh Beyoğlu’nun değişen
yaşamını anlatan 12 bölümlük bir kısa dizidir. Alzheimer hastalığı tanısı aldıktan sonra
Agâh Beyoğlu seri cinayetler işlemeye başlar. Bu makalenin ilk bölümünde, dizinin ana
karakterleri ve hikâye örüntüsü genel hatlarıyla özetlenmiştir. Çalışmanın ikinci
bölümünde ise dizinin ana karakteri ekseninde Alzheimer hastalığında gözlemlenen
bilişsel süreçlerdeki bozulmalar aktarılmaktadır. Bu kapsamda bellek süreçleri ve
kişilikte ortaya çıkan değişimler, bilişsel psikoloji ve nöroloji disiplinlerinin bulguları
ışığında tartışılmaktadır. Makalenin üçüncü bölümünde, ana karakterin yaşamı ve
hastalığıyla olan ilişkisi klinik çerçeveden ele alınmıştır. Bu bağlamda, Agâh Bey’in
kişilerarası ilişkileri varoluşçu psikoloji çerçevesinden incelenmiştir. Ayrıca, Agâh
Bey’in Alzheimer hastalığı tanısını aldıktan sonra geliştirebileceği düşünülen savunma
mekanizmaları ele alınmıştır. Özetle, bu çalışmada kişinin Alzheimer hastalığı tanısı
almasıyla bilişsel süreçlerindeki bozulmaları nasıl deneyimleyebileceği sunulmuştur.
Ayrıca, Alzheimer hastalığı tanısı aldıktan sonra, Agâh Bey’in yaşayabileceği olası
varoluşsal kaygılar ve kişilerarası çatışmalar tartışılmıştır.
Child Abuse/Neglect and Depressive Symptomatology: The Mediating Roles of Early Maladaptive Schemas
Authors: ÜNAL Elif , GENÇÖZ Tülin
Published: June 2019

Recently, there has been a growing interest in identifying the mechanisms of how child
abuse/neglect predicts the development of psychopathology in adulthood. In that
respect, schema therapy explains the origin of psychopathology with early maladaptive
schemas. This study aimed to explore the mediator roles of five different schema
domains between child abuse/neglect and depressive symptomatology relationship in
a university student sample. The sample of this study consisted of 414 undergraduate
and graduate students from the various universities of Turkey [312 (75.4%) female, and
102 (24.6%) male], whose ages ranged between 18 and 32 (M = 21.69, SD = 2.08).
Parallel Multiple Mediation Analysis for five schema domains showed that among all
schema domains only impaired autonomy/performance schema domain-mediated the
relationship between child abuse/neglect and depressive symptomatology. Individuals
who reported higher levels of childhood abuse and neglect tended to develop more
maladaptive schemas from impaired autonomy/performance schema domain, and as a
consequence, were at increased risk for depressive symptoms. Results were discussed
in light of the relavant literature.
Coexisting bournonite-seligmanite and tennantite-tetrahedrite solid solutions in Darasun gold deposit (Eastern Transbaikalia, Russia): Estimation of the mineral formation temperature
Authors: Lyubimtseva N. G. , Bortnikov N. S. , Borisovsky S. E.
Published: June 2019

Mineral associations, intergrowth features, chemical composition and Sb and As distribution in coexisting fahlore and bournonite-seligmanite solid solutions in the Darasun gold deposit were studied. For the first time the almost complete solid solution was found between bournonite and seligmanite with a continuous isomorphism between Sb and As in the range of Sb/(Sb + As) ratios from 0.21 to 1.00 in the Darasun deposit by EMPA. The composition of the fahlore coexisting with bournonite varies widely: Sb/(Sb + As) ratios 0.03-0.96 and Fe/(Fe + Zn) ratios 0.36-0.87. The coupled compositional evolution from early antimony to late arsenic in coexisting solid solutions has been established. Based on the investigation of the Sb and As distribution between coexisting fahlore and bournonite-seligmanite, the temperatures of their joint deposition and, as a result, of the productive stage, in which they are closely associated with native gold and tellurides (90–335 °С), are estimated.
RANCANG BANGUN SISTIM KENDALI RUMAH CERDAS
Authors: Apollo Apollo , Marhatang Marhatang , Rahman Abdul
Published: June 2019

Masalah utama yang ditemukan pada sektor perumahan adalah aktifitas konsumen dalam menggunakan peralatan listrik dan sistim kendali kelistrikan yang kurang efisien yang dikelola oleh PLN.Tujuan penelitian ini adalah merancang bangun suatu sistem kendaliterintegrasi pada rumah hunian(smarthome) dan menganalisis penggunaan energi tahunannya. Metode yang digunakan dalam rancang bangun ini adalahmetode eksperimentalberupa pembuatan rumahminiatur dengan lima zona kendali penerangan dan dua zona kendali pendingin, dan metode simulasi aktifitaspenghuni rumah menggunakan mikrokontroler ATMega8535. Temperatur pendingin dikendalikan dengan suatu sistim sensor temperatur dan intensitas lampu penerangandiatur oleh LDR (light defendent relay) berdasarkan jam operasionalpenghuni rumah. Seluruh kendali smarthomedikelola oleh mikrokontroller danprogram antarmuka CodeVision AVR. Berdasarkan hasil analisis konsumsi energi listrik tahunan, sistim rumah cerdas ini dapat menghemat pemakaian energi hingga 18,799%.
Discriminación bilateral de la respuesta condicionada de parpadeo en humanos
Authors: Pinto Pinto Jorge Andrés , Becerra Céspedes Sebastián Agustín
Published: June 2019

The present investigation aims to determine if the conditioned response of eyeblink is lateralized in humans. For this an experiment was carried out, where the participants received a total of 132 tests consisting of the presentation of auditory stimuli and vibrations, of which 66 trials corresponded to the conditioned stimulus (CS+) associated with an air puff as unconditioned stimulus (US), alternated with 66 tests of the conditioned stimulus (CS-) only. The responses were measured both to the eye that received stimulation (ipsilateral) and to the one that did not receive it (contralateral) during the course of training. The results showed that the responses are bilateral, which would not allow separating the sensory effects of the emotional ones in an CS-US association.
La aplicación de modelos lineales jerárquicos para el estudio de la eficacia en psicoterapia
Authors: Gómez Penedo Juan Martín , Muiños Roberto , Hirsch Pablo , Roussos Andrés
Published: June 2019

Hierarchical Linear Models (HLM) represents a valuable statistical tool for psychotherapy research, given that they allow dealing with the usual dependency presented in its data. These methods are useful to estimate change, disaggregate sources of variations, and analyze the effect of different level predictors. Considering that, these analyses required a highly sophisticated technical knowledge that might remain inaccessible for many researchers, the aim of this paper is to present a guide on how to understand, apply, and report HLM for psychotherapy effects research. To illustrate how to apply HLM, we have drawn on a naturalistic clinical dataset. Disseminating these methods in the Latin-America might represent a meaningful contribution both for research and practice, improving the soundness of clinical studies and helping to develop a more robust knowledge that might leads to greater process and outcome in psychotherapy.
Estructura interna de la Multidimensional Scale of Perceived Social Support (MSPSS) en adolescentes peruanos
Authors: Navarro-Loli Jhonatan Steeven , Merino-Soto Cesar , Dominguez-Lara Sergio , Lourenço Abilio
Published: June 2019

The aim of the study is to identify the evidence of the validity of the internal structure and the relationship with other variables of the MSPSS. The samples were formed by 242 students (Myears old = 14.02, DEyears old = 1,272) and 271 students (Myears old = 15.94, DEyears old = 1.192). In phase one, the original factorial structure of three oblique factors (family, friends and significant others) was replicated, and in the second phase it was corroborated that the dimensions of the MSPSS are predictors of eutymy (positive affect) and dysthymia (negative affect) of the state/Trait Depression Inventory. Reliability indicators of the scores and the construct are adequate. It is concluded that the MSPSS is an instrument with psychometric properties and that it can be used in the educational context.
Umkämpfte Arbeit in der Automobil-Industrie
Authors: Burmeister Kai
Published: June 2019

Der Beitrag setzt sich aus gewerkschaftlicher Perspektive mit der anstehenden Transformation der Automobilindustrie und den zu erwartenden Konflikten rund um Arbeit auseinander. In einem ersten Schritt werden die Besonderheiten der Automobilwirtschaft und die Struktur der Beschäftigung im Automobilcluster Baden-Württemberg dargestellt. Darauf aufbauend erfolgt eine kritische Auseinandersetzung mit der Strategie der hiesigen Automobilindustrie unter der Fragestellung: „Ist die Industrie ausreichend auf die Zukunft vorbereitet?“. Im dritten Schritt werden unterschiedliche Arenen umkämpfter Arbeit in Verbindung mit betriebs-, branchen- und strukturpolitischen Ansätzen dargestellt sowie der Zusammenhang von Arbeit und Ökologie problematisiert. Der Abschluss bildet ein zusammenfassender Ausblick.
Editorial
Authors: Redaktion PROKLA
Published: June 2019

Editorial: Umkämpfte Arbeit – reloaded
The 70 year anniversary of the Roczniki Państwowego Zakładu Higieny / Annals of the National Institute of Hygiene
Authors: Ćwiek-Ludwicka Kazimiera
Published: June 2019

The article presents the genesis of the foundation and development of ‘Roczniki Panstwowego Zakładu Higieny’ [Annals of the National Institute of Hygiene] since 1950 to 2019, scientific peer-reviewed journal devoted to research studies on the food and water safety, nutrition, environmental hygiene, toxicology and health risk assessment, and public health. It also shows the difficulties that this journal had initially to struggle with, and its achievements in recent years, aiming to improve its international position. The article discusses the stages of journal’s development and activity in terms of scientific, editorial and publishing
Selected determinants of eating behaviours among preschool children from the Kraków environment
Authors: Gacek Maria
Published: June 2019

Background. One of the factors determining the nutritional behaviour of children and adolescents is family socio-economic status.
Objective. The aim of the study was to assess the nutritional behaviour of preschool children from the Kraków environment depending on the sex of the child and the mother’s level of education.
Material and methods. The study was carried out among a group of mothers of 480 (240 girls and 240 boys) 4-6-yearolds, using the author’s questionnaire on dietary behaviours. Children of mothers with higher education accounted for 63.5%, and those with primary, vocational and secondary education constituted 36.5% of the group. Statistical analysis was carried out using the PQStat package 1.6.6.246 version, applying Pearson’s Chi-squared test (p<0.05).
Results. The girls consumed a significantly greater amount of: vegetables (p<0.01), fruit (p<0.05), wholemeal bread (p<0.001) as well as milk and dairy products (p<0.001), and boys: meat and cold-cuts from poultry (p<0.001) and sweets (p<0.05). Children of mothers with higher education more frequently consumed: vegetables (p<0.001), fruit (p<0.001), wholemeal bread (p<0.001), fish (p<0.01) and eggs (p=0.01), and children of mothers with secondary and vocational education: milk and dairy products (p<0.05), meat and cold-cuts from poultry (p<0.001) and sweets (p=0.001), salted snacks (p<0.001) and sweetened fizzy beverages (p<0.05).
Conclusions. Diversity in some dietary choices of preschool children from the Kraków environment has been demonstrated, depending on the sex of children and the mother’s level of education. Furthermore, it is indicated that girls and the children of mother’s with higher education are characterised by a more favourable diet.
Advanced Laryngotracheal Stenosis in a Tertiary Provincial Government Hospital: A Prospective Case Series
Authors: Villanueva Jules Verne M. , Soriano Ronaldo G.
Published: June 2019

Objective: To describe the clinical profiles, interventions, and surgical outcomes of patients with advanced (grade III and IV) laryngotracheal stenosis prospectively seen over a 2-year period.  

Methods:

            Design:           Prospective Case Series

            Setting:           Tertiary Provincial Government Hospital

            Participants:  Five (5) patients with advanced laryngotracheal stenosis       confirmed by laryngoscopy and/or tracheoscopy.

Results: Five (5) patients (4 males, 1 female), aged 23 to 31years (mean 27-years-old) diagnosed with advanced laryngotracheal stenosis between June 2016 to June 2018 were included in this series. Four resulted from prolonged intubation (14 - 60 days) while one had a prolonged tracheotomy (13 years). Presentations of stenosis included dyspnea on extubation attempt (n=3), failure to extubate (n=1) and failure to decannulate tracheotomy (n=1). Stenosis length was 3 cm in two, and 1.5 cm in three. Of the five (5) patients, three had grade IV stenosis while two had grade III stenosis based on the Cotton-Myer Classification System. Two of those with grade IV stenosis and both patients with grade III stenosis had undergone prolonged intubation. The stenosis involved the subglottis in three, and combined subglottic and tracheal stenosis in two. Prolonged intubation was present in all three with subglottic stenosis, and in one of the two with combined subglottic and tracheal stenosis. Two patients underwent open surgical approaches while three underwent endoscopic dilatation procedures. Four patients were successfully decannulated while one is still on tracheostomy. None of them had post-operative complications.

Conclusion:  Advanced laryngotracheal stenosis is a challenging entity that results from heterogenous causes. Categorizing stenosis and measuring stenosis length may help in treatment planning and predicting surgical outcome.

 Keywords: laryngotracheal stenosis; laryngotracheal reconstruction; tracheal resection anastomosis; subglottic stenosis; tracheal stenosis
Non-Traumatic Cerebrospinal Fluid Leak from a Sphenoid Sinus Midline Roof Defect Previously Managed as Allergic Rhinitis
Authors: Formalejo Jan Paul D. , Amable Jay Pee M.
Published: June 2019

Objective: To present a case of a non-traumatic cerebrospinal fluid (CSF) rhinorrhea from a midline sphenoid sinus roof that presented as a persistent postnasal drip and was previously managed as allergic rhinitis for 43 years.

 

Methods:

            Design:           Case Report

            Setting:           Tertiary Private University Hospital

            Participants:   One

 

Results: A 58-year-old obese and hypertensive man presented with persistent post nasal drip and intermittent clear watery rhinorrhea. He had been managed as a case of allergic rhinitis for 43 years and was maintained on nasal steroid sprays without relief. Nasal endoscopy revealed pulsating clear watery discharge from the sphenoid ostium. On trans-sphenoidal surgery, a midline sphenoid sinus roof defect was sealed using a Hadad-Bassagasteguy flap.

 

Conclusion: CSF rhinorrhea is uncommon and may mimic more common diseases such as allergic rhinitis. Because misdiagnosis can then lead to life threatening complications, physicians should be vigilant when seeing patients with clear watery rhinorrhea to be able to arrive at a proper diagnosis and provide prompt treatment.

 

Keywords: cerebrospinal fluid rhinorrhea; cerebrospinal fluid leak; sphenoid sinus midline roof defect
Autologous Tracheal Cartilage Composite Graft for a Subglottic Defect after Laryngotracheal Resection for Invasive Papillary Thyroid Carcinoma
Authors: Crisostomo Miguel V. , Ureta Celso V.
Published: June 2019

Objective: To present a function-preserving surgical technique of post-laryngotracheal resection reconstruction of the subglottic airway using autologous tracheal cartilage composite graft.

Methods:

          Design:            Case Report

          Setting:                       Tertiary Government Training Hospital

          Participants:              One

Results: A 77-year-old woman diagnosed with papillary thyroid carcinoma with laryngotracheal invasion underwent total thyroidectomy with laryngotracheal resection. The tracheal defect was reconstructed using end-to-end anastomosis of the trachea to the remaining cricoid. The cricoid (subglottic) defect was repaired using the harvested tracheal cartilage with mucosa. Post-operatively, the patient was maintained on nasogastric tube feeding and tracheostomy tube for 2 weeks. Subsequently, the nasogastric tube and tracheostomy tube were removed and the  patient tolerated oral feeding without any airway problem. The last follow-up of the patient was 6 months post-operatively without complications.

Conclusion: Autologous tracheal cartilage may be a potentially promising composite graft for reconstruction of the cricoid (subglottic) defect in a patient following laryngotracheal resection for invasive papillary thyroid carcinoma of the larynx and trachea.

 

Keywords: tracheal composite graft; laryngotracheal resection; crico-tracheal anastomosis; papillary thyroid carcinoma; subglottic defect
Profiling of Mycoplasma gallisepticum Ribosomes
Authors: Fisunov G. Yu. , Evsyutina D. V. , Arzamasov A. A. , Butenko I. O. , Govorun V. M.
Published: June 2019

The development of high-throughput technologies is increasingly resulting in identification of numerous cases of low correlation between mRNA and the protein level in cells. These controversial observations were made on various bacteria, such as E. coli, Desulfovibrio vulgaris, and Lactococcus lactis. Thus, it is important to develop technologies, including high-throughput techniques, aimed at studying gene expression regulation at the level of translation. In the current study, we performed proteomic profiling of M. gallisepticum ribosomes and identified high abundant noncanonical proteins. We found that binding of mRNAs to ribosomes is mainly determined by two parameters: (1) abundance of mRNA itself and (2) complimentary interactions between the 3 end of 16S rRNA and the ribosome binding site in the 5-untranslated region of mRNA.
Mutations in the Parkinson’s Disease-Associated PARK2 Gene Are Accompanied by Imbalance in Programmed Cell Death Systems
Authors: Konovalova E. V. , Lopacheva O. M. , Grivennikov I. A. , Lebedeva O. S. , Dashinimaev E. В. , Khaspekov L. G. , Fedotova E. Yu. , Illarioshkin S. N.
Published: June 2019

Parkinsons disease is caused by the degeneration of midbrain dopaminergic neurons. A rare recessive form of the disease may be caused by a mutation in the PARK2 gene, whose product, Parkin, controls mitophagy and programmed cell death. The level of pro- and anti-apoptotic factors of the Bcl-2 family was determined in dopaminergic neurons derived from the induced pluripotent stem cells of a healthy donor and a Parkinsons disease patient bearing PARK2 mutations. Western blotting was used to study the ratios of Bax, Bak, Bcl-2, Bcl-XL, and Bcl-W proteins. The pro-apoptotic Bak protein level in PARK2-neurons was shown to be two times lower than that in healthy cells. In contrast, the expression of the anti-apoptotic factors Bcl-XL, Bcl-W, and Bcl-2 was statistically significantly higher in the mutant cells compared to healthy dopaminergic neurons. These results indicate that PARK2 mutations are accompanied by an imbalance in programmed cell death systems in which non-apoptotic molecular mechanisms play the leading role.
Induced Pluripotent Stem Cells of Microtus levis x Microtus arvalis Vole Hybrids: Conditions Necessary for Their Generation and Self-Renewal
Authors: Grigor’eva E. V. , Shevchenko A. I. , Medvedev S. P. , Mazurok N. A. , Zhelezova A. И. , Zakian S. M.
Published: June 2019

Every year, the list of mammalian species for which cultures of pluripotent stem cells (PSCs) are generated increases. PSCs are a unique tool for extending the limits of experimental studies and modeling different biological processes. In this work, induced pluripotent stem cells (iPSCs) from the hybrids of common voles Microtus levis and Microtus arvalis, which are used as model objects to study genome organization on the molecular-genetic level and the mechanisms of X-chromosome inactivation, have been generated. Vole iPSCs were isolated and cultured in a medium containing cytokine LIF, basic fibroblast growth factor (bFGF), ascorbic acid, and fetal bovine serum. Undifferentiated state of vole iPSCs is maintained by activation of their endogenous pluripotency genes - Nanog, Oct4, Sox2, Sall4, and Esrrb. The cells were able to maintain undifferentiated state for at least 28 passages without change in their morphology and give rise to three germ layers (ectoderm, mesoderm and endoderm) upon differentiation.
Valproic Acid Increases the Hepatic Differentiation Potential of Salivary Gland Cells
Authors: Petrakova O. S. , Ashapkin V. V. , Shtratnikova V. Yu. , Kutueva L. I. , Vorotelyak E. A. , Borisov M. A. , Terskikh V. V. , Gvazava I. G. , Vasiliev A. V.
Published: June 2019

The studies of cell plasticity and differentiation abilities are important problems in modern cellular biology. The use of histone deacetylase inhibitor - valproic acid is a promising approach to increasing the differentiation efficiency of various cell types. In this paper we investigate the ability of mouse submandibular salivary gland cells to differentiate into the hepatic direction and the effect of valproic acid on the efficiency of this differentiation. It was shown that the gene expression levels of hepatocyte markers (Aat, Afp, G6p, Pepck, Tat, Cyp3a13) and liver-enriched transcription factors (Hnf-3, Hnf-3, Hnf-4, Hnf-6) were increased after differentiation in salivary gland cells. Valproic acid increases the specificity of hepatic differentiation, reducing the expression levels of the ductal (Krt19, Hhex1, Cyp7a1) and acinar (Ptf1a) markers. After valproic acid exposure, the efficiency of hepatic differentiation also increases, as evidenced by the increase in the gene expression level of Alb and Tdo, and increase in urea production by differentiated cells. No change was found in DNA methylation of the promoter regions of the genes; however, valproic acid treatment and subsequent hepatic differentiation largely affected the histone H3 methylation of liver-enriched genes. Thus, mouse submandibular salivary gland cells are capable of effective differentiation in the hepatic direction. Valproic acid increases the specificity and efficiency of the hepatic differentiation of these cells.
Recombinant Immunotoxin 4D5scFv-PE40 for Targeted Therapy of HER2-Positive Tumors
Authors: Sokolova E. A. , Stremovskiy O. A. , Zdobnova T. A. , Balalaeva I. V. , Deyev S. M.
Published: June 2019

Recombinant immunotoxins are extremely promising agents for the targeted therapy of tumors with a certain molecular profile. In this work, we studied the properties of a new recombinant HER2-specific immunotoxin composed of the scFv antibody and a fragment of Pseudomonas exotoxin A (4D5scFv-PE40). High affinity of the immunotoxin for the HER2 tumor marker, its selective cytotoxicity against HER2-overexpressing cells, and its storage stability were demonstrated. The 50% inhibitory concentration (IC50) of the 4D5scFv-PE40 immunotoxin for HER2-overexpressing cancer cells was 2.5-3 orders of magnitude lower compared to that for CHO cells not expressing this tumor marker and was 2.5-3 orders of magnitude lower than IC50 of free PE40 for HER2-overexpressing cancer cells. These findings provide a basis for expecting in the long run high therapeutic index values of the 4D5scFv-PE40 immunotoxin for its use in vivo.
Modelagem matemática do sistema de planejamento e controle da produção para redução dos tempos de setup
Authors: Neves CCB
Published: June 2019

Os desafios das organizações vêm aumentando frente a uma situação de mercado globalizado e cada vez mais instigado pelas novas tecnologias e consciências social e ambiental. As demandas de produtos personalizados e complexos exigem uma constante revisão de conceitos e novas adaptações para que as indústrias possam se manter competitivas. Junto à natureza complexa dos Sistemas Produtivos, esse dinamismo exige um processo cada vez mais flexível e rápido, assim como exige aplicação do conceito de produção enxuta, que tem como princípio a eliminação de desperdícios de todos os gêneros: tempos, estoques, espaço, etapas e defeitos. O resultado ótimo é alcançado simultaneamente com o mínimo desperdício, ou seja, obtendo resultado de maneira eficaz e eficiente, quando proposições que auxiliem conclusões são analisadas para se chegar a uma decisão final. No âmbito industrial, essas proposições são analisadas pelo Planejamento e Controle de Produção, que é responsável por receber as informações dos demais setores dentro de uma indústria, abstrair e enfim sintetizar tudo de maneira coerente, garantindo a satisfação de todas as partes da melhor maneira possível. Diante disso, o presente trabalho apresenta uma proposta de modelagem matemática do sistema de Planejamento e Controle da Produção, no que tange à redução dos tempos de preparação da produção (setup). A proposta desenvolvida analisa as variáveis que envolvem uma produção e as compara com as variáveis de outras produções, visando aproveitar ao máximo as variáveis em comum. Para isso, utiliza-se o conceito de distância Euclidiana. Como resposta à aplicação deste modelo, deve haver a redução dos tempos de setup e o auxílio na tomada de decisão na Programação Detalhada da Produção.
The Use of Inside Outside Circle Technique to Improve Student's Writing on Recount Text
Authors: Mutmainah Mutmainah
Published: June 2019

This study is to see the improvement of student’s writing skill on recount text for eight grade students of MTs Al-Khairiyah Pulokencana by using Inside Outside Circle Technique. The subject of this research is the students of Eight Grade of MTs Al-Kahiriyah Pulokencana which consist of 25 students. The method used in this study is Classroom Action Research (CAR) which the researcher works collaboratively with the observer. This study was conducted following kemmis with the following procedure of the research: Planning, Action, Observation and Reflection. The study carried out in two cycles and each cycle consist of two meetings. The observation and the test were the data gathered in this study. The results in this study indicate that there is improvement of the students’ skill in writing recount text. Most of the students gradually gained good score at the end of the cycle. The score of Minimum Mastery Criteion – Kriteria Ketuntasan Minimal (KKM) of English lesson was 70.00 (seventy).  The researcher analyzes the writing component containing content, organization, vocabulary, languge use and mechanics. The mean score in cycle I is 65,36 and the mean score in cycle II is 76. Based on the mean score of both cycle, the mean score in cycle II is higher than cycle I. So there is improvement of writing skill of the second grade students of MTs Al-Khairiyah Pulokencana after being taught by using Inside Outside Circle Technique.
Critical Literacy Based Instructional Reading Materials
Authors: Rohadi Tedi
Published: June 2019

ABSTRACT

The study aims at developing and producing a set of instructional materials used as a model of teaching/learning materials of critical reading. The model is developed through stages and procedures of research and development methods. The results of the study show that;  a) the existing instructional materials in certain extent referring to students’ and lecturers incompatibility requires variation in terms of new reading approach, skills to be trained, and types of activities; b) the type of critical reading to be developed is critical literacy; c) the models resulted from the study are theoretically and practically eligible and feasible in accordance to a panel of  expert judgement; d) the models are empirically proven effective to improve  student's skills in reading critically; e)  the final models comprise of students learning materials, teachers guidance/manual, and some supporting materials.
Using Feedback in Teaching Writing Descriptive Text
Authors: Muthoharoh Nada Raudhotul , Anita Anita
Published: June 2019

This study is to see the improvement of student’s writing skill on descriptive text for seventh grade students of SMP 14 Kota Serang by using peer feedback technique. The subject of this research is the students of seventh grade of SMP 14 Kota Serang which consist of 30 students. This research is applied by a quasi-experimental design and quantitative research method. The instrument used in this research was tests (pre-test and post-test) which is conducted based on the main problems: 1) How is the students writing ability on descriptive text at the seventh grade of SMP 14 Kota serang? 2) How is the effectiveness of peer feedback in teaching writing descriptive text at the seventh grade of SMP 14 Kota serang?. The research finding of the last result showed that “to” from the calculation is higher than t table both at significance level 5% and 1%. Significance 5% t observation= 7.14 and t table= 1.67 or 7.14 >1.67 which its significance 1% t observation=7.14 and t table= 2.39 or 7.14 >2.39. In addition, the result of observations showed that the students more motivated in learning English, especially for writing subject. Based on the result above, it can be concluded that peer feedback can be used in teaching writing descriptive text. The result of this research shows that the students’ writing on descriptive text who use peer feedback achieve better performance than those who do not use it.
Development of Problem Based Learning (PBL) practicum guide to improve student Creative Thinking Skills (CTS) in basic physics subject
Authors: Sahida Desrianti , Zarvianti Enny
Published: June 2019

The current study is research and development study, conducted based on observation results obtained from STKIP Muhammadiyah Sungai Penuh. 4-D model was applied in this study. The 4-D model consists of (1) define. Curriculum analysis was conducted, resulting in 48 credits obtained. Analysis on students was conducted, resulting in data revealing that the students’ creative thinking skills are still categorized as low. Analysis on subject matters was conducted, resulting in decision to implement the study in Basic Physics I subject matter.(2) design, where the preliminary practicum guide was obtained. (3) development, where the PBL-based practicum guide was categorized as 0.84% valid, 92.5% practical, 77% effective for cognitive aspects, competent for CTS improvements, 80% creative, 84% competent for attitudes and 84% competent for skills (4) dissemination, where the PBL-based practicum guide was categorized as 92% practical, 76.9% effective for cognitive aspects, competent for CTS improvements, 70% creative, 84% competent for attitudes and 86% competent for skills.
THE CHARACTERISTICS OF ADOLESCENTS NASOPHARYNGEAL CARCINOMA PATIENTS IN MOHAMMAD HOESIN GENERAL HOSPITAL PALEMBANG JANUARY 2013 -DECEMBER 2017
Authors: Melania  , Utama Denny Satria
Published: June 2019

Introduction

Nasopharyngeal carcinoma is (NPC) mostly found in men of productive age with a ratio of men and women 2,18: 1 and 60% of patients age between 25 and 60 years. NPCs are very rarely found in adolescents, namely age 11 to 21 years.

Objective

To determine the characteristics of nasopharyngeal carcinoma in adolescent patients at ORL-HNS Department of Dr. Mohammad Hoesin General Hospital Palembang.

Method

This is a descriptive retrospective study. The sample is all patients diagnosed with NPC in adolescence at ORL-HNS Department of Dr. Mohammad Hoesin General Hospital Palembang in January 2013 to December 2017 period.

Results

 In this study, the number of adolescent NPC patients from January 2013 to December 2017 was 12 patients. The majority of adolescent NPC patients were male (66,6%). The most common histopathological finding was WHO 2B (50%). Based on the T category, largely, the patients were T4 (41,6%). Most patients with Nodules were N3 (58,3%) and came to the ENT clinic were already in stage IV (41,6%).

Conclusion

Adolescent nasopharyngeal carcinoma is more common in men than women (2: 1), and shows a response to proper therapy.
A New Decomposition Method for Designing Microservices
Authors: Al-Debagy Omar , Martinek Peter
Published: June 2019

Many companies are migrating from monolithic architectures to microservice architectures, and they need to decompose their applications in order to create a microservices application. Therefore, the need comes for an approach that helps software architects in the decomposition process. This paper presents a new approach for decomposing monolithic application to a microservices application through analyzing the application programming interface. Our proposed decomposition methodology uses word embedding models to obtain word representations using operation names, as well as, using a hierarchical clustering algorithm to group similar operation names together in order to get suitable microservices. Also, using grid search method to find the optimal parameter values for Affinity Propagation algorithm, which was used for clustering, as well as using silhouette coefficient score to compare the performance of the clustering parameters. The decomposition approach that was introduced in this research consists of the OpenAPI specifications as an input, then extracts the operation names from the specifications and converts them into average word embedding using fastText model. Lastly the decomposition approach is grouping these operation names using Affinity Propagation algorithm. The proposed methodology presented promising results with a precision of 0.84, recall of 0.78 and F-Measure of 0.81.
HUBUNGAN EKSPRESI PENUANGAN TARI BEDHAYA DENGAN KOREOGRAFI BEDHAYA SARPA RODRA SUSUNAN SARYUNI PADMININGSIH
Authors: Lokanantasari Ryndhu Puspita , Slamet S.,
Published: June 2019

Tari Bedhaya Sarpa Rodra merupakan karya tari model bedhaya yang diciptakan di luar istana. Tari ini menggambarkan keserakahan manusia yang diwujudkan pada tokoh Sarpakenaka. Sajian koreografinya menghadirkan keunikan terutama pada gerak dan musik tarinya. Untuk itu, perlu dianalisis bentuk tari bedhaya
dan proses pembentukan koreografinya dengan pisau analisis konsep bentuk Suzanne K. Langer, konsep Hastasawanda, dan teori Effortshape, serta aspek koreografi kelompok Y. Sumandiyo Hadi.
Kata kunci: bedhaya, Bedhaya Sarpa Rodra.

The dance Bedhaya Sarpa Rodra is a dance work of bedhaya model created outside the palace. The dance shows human greedmanifested in the figureSarpakenaka. The choreography presents uniqueness especially
on the movement and the dance music. For the reason, the form of dance Bedhaya and the composing process of choreography need to be analyzed by the concept of form by Suzanne K. Langer, concept of Hastasawanda, and Effortshape theory, as well as aspect of group choreography by Y. Sumandiyo Hadi.
Keywords: Bedhaya, Bedhaya Sarpa Rodra.
MITOS TOKOH PEREMPUAN LAKON ABDULMULUK JAUHARI TEATER DULMULUK TUNAS HARAPAN
Authors: Handayani Tri Puji , Murtana I Nyoman
Published: June 2019

Penelitian berjudul “Mitos Tokoh Perempuan Lakon Abdulmuluk Jauhari Teater Dulmuluk Tunas Harapan” ini menganalisis tentang tokoh perempuan yang dimainkan oleh aktor laki-laki dan mitos tokoh perempuan dalam lakon Abdulmuluk Jauhari. Dengan menggunakan teori semiotika untuk menjawab permasalahan tersebut maka peneliti membuat pertanyaan dalam rumusan masalah yang berkaitan dengan: bagaimana materi mitos wicara tokoh perempuan dalam lakon Abdulmuluk Jauhari pertunjukan teater Dulmuluk kelompok Tunas Harapan. Penelitian ini menggunakan teori semiotika Roland Barthes untuk melihat analisis tokoh perempuan dalam lakon Abdulmuluk Jauhari. Hasil penelitian ini memberikan pemahaman yaitu (1) Tokoh perempuan yang dimainkan oleh aktor laki-laki menampilkan sosok perempuan dengan menggunakan kostum, make-up, hair style, gerak dan nada yang menyerupai perempuan. Tunas Harapan dalam menampilkan tokoh perempuan melalui aktor laki-laki memiliki simbol-simbol kebudayaan yang disampaikan melalui tokoh perempuan. (2) Tunas Harapan menampilkan tokoh perempuan berdasarkan ideologi gender yang menghadirkan mitos perempuan, yaitu perempuan sebagai kesetaraan gender, perempuan sebagai sosok pemberani, perempuan sebagai sosok penguasa, dan perempuan sebagai penyelamat.

Kata kunci: Teater Dulmuluk, Tokoh Perempuan, Mitos.

The research entitled “ Mitos Tokoh Perempuan Lakon Abdulmuluk Jauhari Teater Dulmuluk Tunas Harapan “ analyzed the female characters played by male actors and the myth of a female character in Abdulmuluk Jauhari’s play. By using semiotic theory to answer these problems, the researcher makes a question in the formulation of the problem related to: how is the material of the myth of the talk of female characters in Abdulmuluk Jauhari’s play Dulmuluk theater group Tunas Harapan. This study uses Roland Barthes’s semiotic theory to look at the analysis of female characters in Abdulmuluk Jauhari’s play. The results of this study provide understanding, namely (1) The female figure played by male actors displays a female figure using costumes, make-up, hair styles, movements and tones that resemble women. Tunas Harapan in presenting female characters through male actors has cultural symbols conveyed through female characters. (2) Tunas Harapan presents women leaders based on gender ideology that presents myths of women, namely women as gender equality, women as brave figures, women as figures of rulers, and women as saviors.

Keywords: Theater Dulmuluk, Female Leader, Myth.
KLEWERAN SASTRO KINANG SEBUAH KARYA SENI PENCIPTAAN TARI
Authors: Dewi Maharani Luthvinda , Supriyanto Eko
Published: June 2019

Karya Kleweran Sastro Kinang diinspirasi dari bango Sastro Kinang yang pernah berada di Los C10-11 Pasar Klewer. Karya ini berupaya menampilkan aspek performatif yang natural dan artifisial. Aspek natural terjadi melalui aktivitas transaksi pedagang dan pembeli. Aspek artifisial disajikan melalui tari yang geraknya merupakan hasil eksplorasi laku pedinan di Pasar Klewer. Keseluruhan aspek itu diwadahi dalam karya dengan bentuk pameran atau instalasi aneka produk Pasar Klewer.

Kata kunci: Klewer, Sastro Kinang, laku pedinan.

The work of Kleweran Sastro Kinang was inspired by Bango Sastro Kinang who was at Los C10-11 Pasar Klewer. This work seeks to display the natural and artificial performative aspects. Natural aspects occur through transaction activities of traders and buyers. An artificial aspect is presented through dance whose motion is the result of laku pedinan exploratory in the Klewer Market. All aspects are accommodated in works with exhibition forms or various installations of Klewer Market products.

Keywords: Klewer, Sastro Kinang, laku pedinan.
Perawatan Metode Kanguru (PMK) Mempersingkat Lama Rawat Bayi Baru Lahir
Authors: Suryadi Bambang , Fitri Yohana Yuniarti
Published: June 2019

Kematian bayi merupakan bagian dari salah satu masalah bagi kesehatan, salah satu faktor yang menyebabkan hal tersebut yaitu Bayi Berat Lahir Rendah (BBLR) yang mengakibatkan lama rawat, hal yang dapat dilakukan untuk mengatasi tindakan yang dilakukan yaitu dengan melakukan Perawatan Metode Kangguru (PMK). Tujuan dari penelitian ini untuk mengetahui Hubungan PMK dengan lama rawat pada BBLR. Penelitian ini menggunakan analitik deskriptif dengan pendekatan retrospektif pada 99 responden. Uji statistic dalam penelitian ini menggunakan chi square dengan teknik pengambilan sampel yang digunakan peneliti adalah total sampling. Hasil penelitian didapatkan bahwa mayoritas responden melakukan PMK rutin sebanyak 83,8% dan lama rawat singkat sebanyak 65,7% terdapat hubungan yang signifikan antara PMK dengan lama rawat pada BBLR  (P=0,001). Hal tersebut dapat dijadikan rekomendasi pemberian edukasi bahwa PMK rutin dilakukan untuk mempersingkat lama rawat.
Museo de Cádiz. Títeres y el teatro de títeres La Tía Norica
Authors: Cornejo Francisco J.
Published: June 2019

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