bluuebunny/crossref_metadata_2025 huggingface dataset:
Biorobotic Drug Delivery for Biomedical Applications
Authors: Le Quoc-Viet , Shim Gayong
Published: August 2024

Despite extensive efforts, current drug-delivery systems face biological barriers and difficulties in bench-to-clinical use. Biomedical robotic systems have emerged as a new strategy for drug delivery because of their innovative diminutive engines. These motors enable the biorobots to move independently rather than relying on body fluids. The main components of biorobots are engines controlled by external stimuli, chemical reactions, and biological responses. Many biorobot designs are inspired by blood cells or microorganisms that possess innate swimming abilities and can incorporate living materials into their structures. This review explores the mechanisms of biorobot locomotion, achievements in robotic drug delivery, obstacles, and the perspectives of translational research.
Experimental and In Silico Comparative Study of Physicochemical Properties and Antimicrobial Activity of Carboxylate Ionic Liquids
Authors: Cakó Bagány Nikolett , Čapelja Eleonora , Kovačević Strahinja , Karaman Maja , Podunavac-Kuzmanović Sanja , Gadžurić Slobodan , Belić Sanja
Published: August 2024

The COVID-19 pandemic highlighted the need to create and study new substances with improved lipophilicity and antimicrobial properties, such as ionic liquids (ILs), with easily tunable physicochemical properties. Most ILs possess strong antibacterial effects, but ILs containing the imidazolium cation are even more effective than the positive control. Thus, in this study, three ionic liquids with 1-butyl-3-methylimidazolium cation and various carboxylate anions (phenylacetate, benzoate, and 4-methoxyphenylacetate) were synthesized and fully characterized. The interactions between the cations and anions were discussed based on the experimental density, viscosity, and electrical conductivity. From the measured electrical conductivity and viscosity, the Walden plot is constructed and ionicity of the studied ILs is discussed. The similarities and dissimilarities among the studied ILs and their physicochemical properties are analyzed by applying the hierarchical cluster analysis and in silico calculated properties. The antimicrobial activity of the studied ionic liquids is tested on two bacterial (E. coli and P. aeruginosa) and three fungi (P. verrucosum, A. flavus, and A. parasiticus) strains, finding that they showed improved antimicrobial activity compared to the individual components.
Green Approaches for the Extraction of Banana Peel Phenolics Using Deep Eutectic Solvents
Authors: Cegledi Ena , Dobroslavić Erika , Pedisić Sandra , Magnabosca Ivan , Zorić Marija , Pavić Rina , Šuto Marija , Štargl Otilija , Repajić Maja , Elez Garofulić Ivona
Published: August 2024

Banana peels, comprising about 35% of the fruit’s weight, are often discarded, posing environmental and economic issues. This research focuses on recycling banana peel waste by optimizing advanced extraction techniques, specifically microwave-assisted (MAE) and ultrasound-assisted extraction (UAE), for the isolation of phenolic compounds. A choline chloride-based deep eutectic solvent (DES) with glycerol in a 1:3 ratio with a water content of 30% (w/w) was compared to 30% ethanol. Parameters, including sample-to-solvent ratio (SSR), extraction time, and temperature for MAE or amplitude for UAE, were varied. Extracts were analyzed for hydroxycinnamic acid (HCA) and flavonoid content, and antioxidant activity using FRAP and ABTS assays. DES outperformed ethanol, with HCA content ranging from 180.80 to 765.92 mg/100 g and flavonoid content from 96.70 to 531.08 mg/100 g, accompanied by higher antioxidant activity. Optimal MAE conditions with DES were an SSR of 1:50, a temperature of 60 °C, and a time of 10 min, whereas an SSR of 1:60, time of 5 min, and 75% amplitude were optimal for UAE. The polyphenolic profile of optimized extracts comprised 19 individual compounds belonging to the class of flavonols, flavan-3-ols, and phenolic acids. This study concluded that DESs, with their superior extraction efficiency and environmental benefits, are promising solvents for the extraction of high-value bioactive compounds from banana peels and offer significant potential for the food and pharmaceutical industries.
Fast Determination of Eleven Food Additives in River Water Using C18 Functionalized Magnetic Organic Polymer Nanocomposite Followed by High-Performance Liquid Chromatography
Authors: Lei Chao , Zhang Shun , Liu Wen-Xin , Ye Ming-Li , Zhao Yong-Gang
Published: August 2024

A novel magnetic nanomaterial with Fe3O4 as the core, PS-DVB as the shell layer, and the surface modified with C18 (C18−PS−DVB−Fe3O4) had been synthesized by seeded emulsion polymerization. C18−PS−DVB−Fe3O4 retains the advantages of the chemical stability, large porosity, and uniform morphology of organic polymers and has the magnetic properties of Fe3O4. A simple, flexible, and efficient magnetic dispersive solid phase extraction (Mag-dSPE) method for the extraction of preservatives, sweeteners, and colorants in river water was established. C18−PS−DVB−Fe3O4 was used as an adsorbent for Mag-dSPE and was coupled with high-performance liquid chromatography (HPLC) to detect 11 food additives: acesulfame, amaranth, benzoic acid, tartrazine, saccharin sodium, sorbic acid, dehydroacetic acid, sunset yellow, allura red, brilliant blue, and erythrosine. Under the optimum extraction conditions, combined with ChromCoreTMAQC18 (5 μm, 4.6 × 250 mm), 20 mmol/L ammonium acetate aqueous solution and methanol were used as mobile phases, and the detection wavelengths were 240 nm and 410 nm. The limits of detection (LODs) of 11 food additives were 0.6–3.1 μg/L with satisfactory recoveries ranging from 86.53% to 106.32%. And the material could be reused for five cycles without much sacrifice of extraction efficiency. The proposed method has been used to determine food additives in river water samples, and results demonstrate the applicability of the proposed C18−PS−DVB−Fe3O4 Mag-dSPE coupled with the HPLC method to environment monitoring analysis.
Optimizing Geometry and ETL Materials for High-Performance Inverted Perovskite Solar Cells by TCAD Simulation
Authors: Gulomova Irodakhon , Accouche Oussama , Aliev Rayimjon , Al Barakeh Zaher , Abduazimov Valikhon
Published: August 2024

Due to the optical properties of the electron transport layer (ETL) and hole transport layer (HTL), inverted perovskite solar cells can perform better than traditional perovskite solar cells. It is essential to compare both types to understand their efficiencies. In this article, we studied inverted perovskite solar cells with NiOx/CH3NH3Pb3/ETL (ETL = MoO3, TiO2, ZnO) structures. Our results showed that the optimal thickness of NiOx is 80 nm for all structures. The optimal perovskite thickness is 600 nm for solar cells with ZnO and MoO3, and 800 nm for those with TiO2. For the ETLs, the best thicknesses are 100 nm for ZnO, 80 nm for MoO3, and 60 nm for TiO2. We found that the efficiencies of inverted perovskite solar cells with ZnO, MoO3, and TiO2 as ETLs, and with optimal layer thicknesses, are 30.16%, 18.69%, and 35.21%, respectively. These efficiencies are 1.5%, 5.7%, and 1.5% higher than those of traditional perovskite solar cells. Our study highlights the potential of optimizing layer thicknesses in inverted perovskite solar cells to achieve higher efficiencies than traditional structures.
Effect of Titanium Dioxide Particles on the Thermal Stability of Silica Aerogels
Authors: Fan Caide , Lu Jialu , Duan Chengjie , Wu Chengbin , Lin Jiming , Qiu Ruoxiang , Zhang Zehui , Yang Jianming , Zhou Bin , Du Ai
Published: August 2024

Silica aerogels exhibit a unique nanostructure with low thermal conductivity and low density, making them attractive materials for thermal isolation under extreme conditions. The TiO2 particle is one of the common industrial additives used to reduce the thermal radiation of aerogel composites under high-temperature environments, but its influence on thermal resistance is almost unknown. Herein, we report the effect of TiO2 nanoparticles with different crystal phases and different sizes on the thermal stability of silica aerogel composites. By adding TiO2 nanoparticles, the aerogel can significantly resist collapse at high temperatures (up to 1000 °C). And compared with the rutile phase TiO2, the anatase phase TiO2 shows much higher temperature resistance performance, with shrinkage of only one-sixth of the rutile phase after 800 °C treatment. Interestingly, energy-dispersive spectrometer mapping results show that after 800 °C treatment, silica nanoparticles (NPs) are squeezed out in between anatase TiO2 particles, which resists the coarsening of silica NPs and ultimately enhances the stability of aerogel composites. The optimal anatase phase TiO2-doped silica aerogel demonstrates the integrated properties of crack-free morphology (2.84% shrinkage), low thermal conductivity (29.30 mW/(m·K)) and low density (149.4 mg/cm3) after 800 °C treatment. This study may provide new insights for developing oxide-doped silica aerogels with both high-temperature resistance and low thermal radiation.
Comment on Cesak et al. Carnosine and Beta-Alanine Supplementation in Human Medicine: Narrative Review and Critical Assessment. Nutrients 2023, 15, 1770
Authors: Child Robert
Published: August 2024

The paper by Cesak et al [...]
Vitamin D Status and Behavioral Impulsivity in Anorexia Nervosa: Insights from a Longitudinal Study
Authors: Todisco Patrizia , De Mico Alberto , Meneguzzo Paolo
Published: August 2024

Anorexia nervosa (AN) is a severe psychiatric disorder marked by extreme weight control behaviors and significant impacts on physical and psychosocial health. This study explores the relationship between vitamin D (Vit-D) levels and impulsivity in women with AN. Forty-six cisgender White women participants were assessed upon admission and before discharge from a specialized eating disorder treatment center, with an average duration of 2.5 ± 0.10 months. Methods included self-reported questionnaires and behavioral tasks to measure impulsivity, alongside serum Vit-D levels. Our results showed significant improvements in Vit-D levels and certain impulsivity measures, such as faster reaction times and fewer errors on the go/no-go task, correlating with higher Vit-D levels. However, no significant correlations were found between Vit-D levels and self-reported impulsivity. These findings suggest that adequate Vit-D levels may enhance cognitive functions related to impulse control in AN. Given this study’s limitations, including its exclusive focus on women and small sample size, future research should involve larger, more diverse populations and randomized clinical trials to better understand the causal relationships and therapeutic potential of Vit-D in managing AN-related impulsivity.
Impact of Lactobacillus acidophilus and Its Combination with Isoflavone Products on Calcium Status, Calcium Transporters, and Bone Metabolism Biomarkers in a Post-Menopausal Osteoporotic Rat Model
Authors: Harahap Iskandar Azmy , Schmidt Marcin , Pruszyńska-Oszmałek Ewa , Sassek Maciej , Suliburska Joanna
Published: August 2024

Osteoporosis in menopausal women requires alternatives to current medications, considering their adverse effects. In this context, probiotics and isoflavone products are promising dietary interventions. The objective of our study was to examine the impacts of Lactobacillus acidophilus and its combination with daidzein and tempeh on calcium status, calcium transporters, and bone metabolism biomarkers in a post-menopausal osteoporotic rat model. A total of 48 female Wistar rats were exposed to a two-stage experiment involving calcium deficit induction and subsequent dietary interventions across six groups. Calcium levels, the gene expression of TRPV5 and TRPV6 calcium transporters, bone histopathology, serum bone metabolism markers, and blood biochemistry were evaluated. The results revealed that, while decreasing serum calcium levels, the groups that received the probiotic L. acidophilus and isoflavone combination exhibited increased bone metabolism biomarkers and decreased calcium transporter expressions, akin to the effects of bisphosphonate. Additionally, significant improvements in bone histopathology were observed in these groups. However, the group receiving probiotic L. acidophilus alone did not exhibit significant changes in bone resorption biomarkers, calcium transporter expression, or various blood parameters. Meanwhile, the combination of probiotic L. acidophilus with tempeh positively influenced hematological parameters and reduced cholesterol and triglyceride levels, but it led to elevated blood glucose levels. Correlation analyses highlighted associations between serum calcium levels, calcium transporter expression, and bone metabolism biomarkers. In conclusion, our findings suggest that the daily consumption of probiotic L. acidophilus in combination with isoflavone products may improve bone health in ovariectomized rats, warranting further research to elucidate potential interactions with other nutrients.
The Effects of Testosterone on Hypothalamic and Serum Oxytocin Levels Are Affected by the Estrogen Milieu in Female Rats
Authors: Arata Moeka , Tamura Kou , Aoki Hidenori , Noguchi Hiroki , Takeda Asuka , Minato Saki , Yamamoto Shota , Kinouchi Riyo , Yoshida Kanako , Yamamoto Yuri , Kaji Takashi , Iwasa Takeshi
Published: August 2024

Previous studies have suggested that the effects of androgens on body weight (BW) and appetite are affected by the estrogen milieu in females; however, the mechanism underlying these effects remains unclear. We hypothesized that androgens may affect endogenous oxytocin (OT), which is a hypothalamic anorectic factor, and that these effects of androgens may be altered by the estrogen milieu in females. To investigate this hypothesis, in the present study, we examined the effects of testosterone on peripheral and central OT levels in ovariectomized female rats that did or did not receive estradiol supplementation. Ovariectomized female rats were randomly divided into non-estradiol-supplemented or estradiol-supplemented groups, and half of the rats in each group were concurrently supplemented with testosterone (i.e., rats were divided into four groups, n = 7 per each group). We also measured peripheral and central OT receptor (OTR) gene expression levels. As a result, we found that testosterone increased serum and hypothalamic OT levels and OT receptor mRNA levels in non-estradiol-supplemented rats, whereas it had no effects on these factors in estradiol-supplemented rats. In addition, testosterone reduced food intake, BW gain, and fat weight in non-estradiol-supplemented rats, whereas it did not have any effects on BW, appetite, or fat weight in estradiol-supplemented rats. These findings indicate that the effects of androgens on OT may be affected by the estrogen milieu, and elevated OT levels may be related to the blunting of appetite and prevention of obesity under estrogen-deficient conditions.
Potential Human Health Benefits of Phaseolus vulgaris L. var Venanzio: Effects on Cancer Cell Growth and Inflammation
Authors: Bernardi Clizia , Cappellucci Giorgio , Baini Giulia , Aloisi Anna Maria , Finetti Federica , Trabalzini Lorenza
Published: August 2024

It is widely recognized that foods, biodiversity, and human health are strongly interconnected, and many efforts have been made to understand the nutraceutical value of diet. In particular, diet can affect the progression of intestinal diseases, including inflammatory bowel disease (IBD) and intestinal cancer. In this context, we studied the anti-inflammatory and antioxidant activities of extracts obtained from a local endangered variety of Phaseolus vulgaris L. (Fagiola di Venanzio, FV). Using in vitro intestinal cell models, we evaluated the activity of three different extracts: soaking water, cooking water, and the bioaccessible fraction obtained after mimicking the traditional cooking procedure and gastrointestinal digestion. We demonstrated that FV extracts reduce inflammation and oxidative stress prompted by interleukin 1β through the inhibition of cyclooxygenase 2 expression and prostaglandin E2 production and through the reduction in reactive oxygen species production and NOX1 levels. The reported data outline the importance of diet in the prevention of human inflammatory diseases. Moreover, they strongly support the necessity to safeguard local biodiversity as a source of bioactive compounds.
Prevention Is Better than Cure—Body Composition and Glycolipid Metabolism after a 24-Week Physical Activity Program without Nutritional Intervention in Healthy Sedentary Women
Authors: Śliwicka Ewa , Popierz-Rydlewska Natalia , Straburzyńska-Lupa Anna , Nikolov Jivko , Pilaczyńska-Szcześniak Łucja , Gogojewicz Anna
Published: August 2024

Women are generally less active than men; therefore, the search for an attractive form of physical activity that benefits women’s health is underway. This study aimed to investigate the influence of a 24-week physical activity program on body composition and indices of carbohydrates and lipid metabolism in sedentary, healthy women. The study comprised 18 female volunteers (mean age 35.0 ± 5.3 years). Dietary intake was assessed using a standardized seven-day food record. Before entering the program and after completing it, each participant’s body composition and indices of glycolipid metabolism were measured. Insulin resistance indexes were calculated based on the obtained data. After the physical activity program, significant decreases in body mass and composition, BMI, waist circumference, percentage of fat content, and fat mass were found. Moreover, there was a significant decrease in glucose, insulin, triglycerides (TG), and resistin concentrations, as well as in the mean values of HOMA-IR and HOMA-AD. A substantial increase in adiponectin levels was also found. To conclude, the combined endurance–resistance physical activity program had a beneficial effect on body mass and composition and improved carbohydrate and lipid metabolism in normal-weight, healthy women. Therefore, we recommend this activity to sedentary young women to prevent obesity and metabolic disorders.
Viral Hepatitis in Pregnant Mexican Women: Its Impact in Mother–Child Binomial Health and the Strategies for Its Eradication
Authors: García-Romero Carmen Selene , Guzmán Carolina , Martínez-Ibarra Alejandra , Cervantes Alicia , Cerbón Marco
Published: August 2024

Viral hepatitis is the main cause of infectious liver disease. During pregnancy, a risk of vertical transmission exists both during gestation and at birth. HAV, HBV, and HCV might progress similarly in pregnant and non-pregnant women. In this study, we found a prevalence of 0.22% of viral hepatitis in pregnant women, with a light preponderance of HCV over HAV and HBV. Here, it was observed that acute HAV infection is more symptomatic and has higher risks for the mother and fetus, in a similar manner to what has been reported for HEV. Histopathological alterations were observed in all except one placenta, indicating that it is an important tissue barrier. Regarding the Mexican strategies for viral hepatitis eradication, success may be related to vaccination at birth, whereas for HCV, the national program for eradication is aimed at treating the infection via direct-acting antiviral agents. The HBV strategy has positively impacted pregnant women and their children, diminishing the risk of vertical transmission. The HCV strategy is still in its early years, and it is expected to be just as successful. For acute hepatitis, HAV and HEV, programs promoting hand washing and those aimed at providing clean food and water are applicable as preventive strategies, alongside other programs such as vaccination.
Utilization of In Vivo Imaging System to Study Staphylococcal Sepsis and Septic Arthritis Progression in Mouse Model
Authors: Deshmukh Meghshree , Hu Zhicheng , Mohammad Majd , Jin Tao
Published: August 2024

Staphylococcus aureus [S. aureus] is a leading cause of sepsis and septic arthritis, conditions that pose significant medical challenges due to their high mortality and morbidity. No studies have used an in vivo imaging system [IVIS] to monitor S. aureus sepsis and septic arthritis. Here, we employed a bioluminescent reporter strain of S. aureus, Newman AH5016, administered intravenously to induce sepsis and intra-articularly to induce local septic arthritis in mice. Disease progression was monitored using IVIS to capture bioluminescent signals from kidneys, joints, and whole mice. Cytokines in the blood and joints were measured. The efficacy of cloxacillin treatment was evaluated. In the sepsis model, bioluminescent signals from kidneys, but not from whole mice, were correlated with kidney bacterial load and abscess formation. Ex vivo kidney imaging detected increased bacterial load and abscess formation from day 3 to day 10. Antibiotic treatment significantly reduced kidney signals, correlating with decreased bacterial counts and IL-6 levels, indicating effective infection control. In the local infection model, early-phase bioluminescent signals from joints were correlated with macroscopic arthritis and bacterial burden. Thus, signal detection from kidneys using IVIS is useful for monitoring S. aureus sepsis and assessing antibiotic efficacy, though it may only be effective for early-phase monitoring of local septic arthritis.
Systemic Factors Affecting Human Beta-Defensins in Oral Cavity
Authors: Atalay Nur , Balci Nur , Gürsoy Mervi , Gürsoy Ulvi
Published: August 2024

Human beta-defensins are host defense peptides with broad antimicrobial and inflammatory functions. In the oral cavity, these peptides are produced mainly by the keratinocytes of the epithelium; however, fibroblasts, monocytes, and macrophages also contribute to oral human beta-defensin expressions. The resident and immune cells of the oral cavity come into contact with various microbe-associated molecular patterns continuously and simultaneously. The overall antimicrobial cellular response is highly influenced by local and environmental factors. Recent studies have produced evidence showing that not only systemic chronic diseases but also systemic factors like hyperglycemia, pregnancy, the long-term use of certain vitamins, and aging can modulate oral cellular antimicrobial responses against microbial challenges. Therefore, the aim of this narrative review is to discuss the role of systemic factors on oral human beta-defensin expressions.
Unveiling the Bioactive Efficacy of Cupressus sempervirens ‘Stricta’ Essential Oil: Composition, In Vitro Activities, and In Silico Analyses
Authors: Fikry Eman , Orfali Raha , Tawfeek Nora , Perveen Shagufta , Ghafar Safina , El-Domiaty Maher M. , El-Shafae Azza M.
Published: August 2024

Prior studies have extensively investigated the essential oil derived from the Mediterranean cypress, Cupressus sempervirens. However, the ‘Stricta’ variety, known for its ornamental value, has received less attention in terms of its oil composition and potential health benefits. The objective of this research was to comprehensively analyze the chemical components and medicinal properties of the essential oil extracted from C. sempervirens ‘Stricta’ (CSSLEO) grown in Egypt. Utilizing gas chromatography–mass spectrometry (GC–MS), the investigation identified 22 compounds within CSSLEO, with α-pinene and δ-3-carene being predominant, accounting for 96.01% of the oil. In vitro assays evaluated CSSLEO’s cytotoxic effects on cancer cell lines, revealing notable anticancer potential. Additionally, the oil displayed antidiabetic properties by impeding crucial enzymes involved in glucose metabolism. Complementary in silico network pharmacology and molecular docking studies provided insights into the possible interactions between CSSLEO’s key compounds and essential proteins and pathways in cancer treatment. The results underscored CSSLEO’s intricate composition and its promising applications in cancer prevention and diabetes management. The conclusions drawn from this research underscore the need for further investigation to validate CSSLEO’s clinical effectiveness and to gain a deeper understanding of its therapeutic mechanisms, with a view to harnessing its potential in oncology and endocrinology.
Nanocrystal Formulation to Enhance Oral Absorption of Silybin: Preparation, In Vitro Evaluations, and Pharmacokinetic Evaluations in Rats and Healthy Human Subjects
Authors: Seo SeungRee , Kim Gwan-Young , Kim Min-Hwan , Lee Kyung Won , Kim Min-Jae , Chaudhary Mansingh , Bikram Khadka , Kim Taeheon , Choi Seungmok , Yang Heejin , Park Joo Won , Kim Dae-Duk , Kim Ki-Taek
Published: August 2024

Despite the various therapeutic benefits and high tolerance of orally administered silybin, poor water-solubility can be the main restrictive physicochemical feature, which results in low oral bioavailability in the absorption. A milk thistle nanocrystal formulation (HM40) was prepared using a modified wet-milling method. Comprehensive characterization was performed to determine the physical morphology, crystallinity, and physicochemical properties. The long-term stability was evaluated over 24 months. In vitro silybin release was assessed at pH 1.2 for 2 h, followed by pH 6.8 for 4 h. Finally, in vivo pharmacokinetic studies were conducted in rats and healthy human volunteers. HM40 exhibited a nanocrystal structure maintaining crystallinity and enhanced the solubility and dissolution of silybin compared to that of the raw material. The stability over 24 months revealed consistent surface morphology, particle size, silybin content, and solubility. In vitro release profiles indicated a significant increase in the silybin release from HM40. In vivo pharmacokinetic studies demonstrated that HM40 showed 2.61- and 1.51-fold higher oral bioavailability in rats and humans, respectively, than that of the reference capsule. HM40 formulation presents a stable and promising approach for the oral delivery of poorly water-soluble silybin, with the potential for use in pharmaceutical formulations containing milk thistle.
Amorphous Solid Dispersions: Implication of Method of Preparation and Physicochemical Properties of API and Excipients
Authors: Kushwah Varun , Succhielli Cecilia , Saraf Isha , Paudel Amrit
Published: August 2024

The present study investigated the effect of different polymers and manufacturing methods (hot melt extrusion, HME, and spray drying, SD) on the solid state, stability and pharmaceutical performance of amorphous solid dispersions. In the present manuscript, a combination of different binary amorphous solid dispersions containing 20% and 30% of drug loadings were prepared using SD and HME. The developed solid-state properties of the dispersions were evaluated using small- and wide-angle X-ray scattering (WAXS) and modulated differential scanning calorimetry (mDSC). The molecular interaction between the active pharmaceutical ingredients (APIs) and polymers were investigated via infrared (IR) and Raman spectroscopy. The in vitro release profile of the solid dispersions was also evaluated to compare the rate and extend of drug dissolution as a function of method of preparation. Thereafter, the effect of accelerated stability conditions on the physicochemical properties of the solid dispersions were also evaluated. The results demonstrated higher stability of Soluplus® (SOL) polymer-based solid dispersions as compared to hydroxypropyl methylcellulose (HPMC)-based solid dispersions. Moreover, the stability of the solid dispersions was found to be higher in the case of API having high glass transition temperature (Tg) and demonstrated higher interaction with the polymeric groups. Interestingly, the stability of the melt-extruded dispersions was found to be slightly higher as compared to the SD formulations. However, the down-processing of melt-extruded strands plays critical role in inducing the API crystal nuclei formation. In summary, the findings strongly indicate that the particulate properties significantly influence the performance of the product.
Photon-Number-Resolving Detection with Highly Efficient InGaAs/InAlAs Single-Photon Avalanche Diode
Authors: Lee Yi-Shan , Chen Tzu-Yang , Chen Yu-Ju , Kan Wei-Hong , Liu Xue-Wen , Shi Jin-Wei
Published: August 2024

Photon-number-resolving detectors are in high demand for applications in photonic quantum technology. In this study, we demonstrate the photon-number-resolving capabilities of our self-developed, highly efficient InGaAs/InAlAs single-photon avalanche diode. We achieved intrinsic photon number resolving by harnessing the high multiplication gain generated through an avalanche process in the InAlAs multiplication layer. With a maximum single-photon detection efficiency of 46%, we were able to distinguish photon number states up to 5 from the signal probability distribution without encountering avalanche saturation that could otherwise limit the capability of photon number resolving. We reasonably anticipate that the photon-number-resolving accuracy and capability can be further improved once the noise issue in such InGaAs/InAlAs SPADs is carefully managed.
In Vitro and In Vivo Antiurolithic Effect of Betulinic Acid Obtained from Citharexylum mirianthum
Authors: Mariano Luísa Nathália Bolda , Vequi Gabriela , da Silva Rita de Cássia Vilhena , Macarini Anelise Felício , Dada Anelize , Costa Thaina Mariz , Omena Murilo Morales , Pereira Christiane Regina Pamplona , Cechinel-Filho Valdir , Niero Rivaldo , de Souza Priscila
Published: August 2024

The study aimed to investigate the potential antiurolithic effects of extracts, fractions, and betulinic acid (BA) from Citharexylum mirianthum. In vitro analysis involved precipitating calcium oxalate (CaOx) crystals in urine. For in vivo studies, rats were divided into four groups: naive; vehicle; potassium citrate (KC); and BA. Urolithiasis was induced using ethylene glycol and ammonium chloride. After seven days, urine, blood, and kidney tissues were evaluated. The results showed that methanolic extract, hexane, dichloromethane, and ethyl acetate fractions, as well as BA, reduced CaOx crystal formation. In vivo, the vehicle-treated group exhibited reduced urinary volume and Na+ excretion, while the BA-treated group showed restored urinary volume and Na+ excretion similar to the naive group. BA also significantly reduced urinary monohydrate and dihydrate crystal formation, comparable to the KC group. Other urinary parameters remained unchanged, but plasma analysis revealed decreased Na+, K+, and Ca2+ in the KC group. Renal tissue analysis indicated reduced lipid hydroperoxides and increased reduced glutathione in all urolithiasis groups, with unchanged nitrite levels. BA treatment also improved renal corpuscle morphology. Overall, our findings demonstrate that treatment with BA effectively prevented kidney damage induced by EG+AC ingestion, thereby improving renal function in the urolithiasis model.
Characterization of Lithuanian Tomato Varieties and Hybrids Using Phenotypic Traits and Molecular Markers
Authors: Radzevičius Audrius , Šikšnianienė Jūratė Bronė , Karklelienė Rasa , Juškevičienė Danguolė , Antanynienė Raminta , Misiukevičius Edvinas , Starkus Aurelijus , Stanys Vidmantas , Frercks Birutė
Published: August 2024

The aim of this study was to evaluate phenotypic traits and genetic diversity of the 13 tomato (Solanum lycopersicum L.) varieties and 6 hybrids developed at the Institute of Horticulture Lithuanian Research Centre for Agriculture and Forestry (LRCAF IH). For the molecular characterisation, seven previously published microsatellite markers (SSR) were used. A24 and 26 alleles were detected in tomato varieties and hybrids, respectively. Based on the polymorphism information content (PIC) value, the most informative SSR primers for varieties were TMS52, TGS0007, LEMDDNa and Tom236-237, and the most informative SSR primers for hybrids were SSR248 and TMS52. In UPGMA cluster analysis, tomato varieties are grouped in some cases due to genetic relationships, as the same cluster cultivars ‘Viltis’ (the parent of cv. ‘Laukiai’) and ‘Aušriai’ (the progeny of cv. ‘Jurgiai’) are present. The grouping of all hybrids in the dendrogram is related to the parental forms, and it shows the usefulness of molecular markers for tomato breeding, as they can be used to trace the origin of hybrids and, eventually, varieties accurately. The knowledge about the genetic background of Lithuanian tomato cultivars will help plan targeted crosses in tomato breeding programs.
Quantitative Analysis of the Impact of Finishing and Washing Processes on the Roughness of Polyester Fabric
Authors: Kalazić Ana , Brnada Snježana , Bušac Tea , Pušić Tanja
Published: August 2024

The roughness of woven fabric surface has so far been mainly investigated as a key characteristic of comfort in contact with the skin. The analysis of roughness can be extended to various contexts and applications, becoming an important tool for understanding how textile materials react in interaction with different finishing agents, as well as for gaining insight into the durability and effectiveness of treatments. This research presents a comprehensive study on the impact of alkaline hydrolysis and chitosan coating on the roughness of polyester woven fabric, utilizing both novel and adapted methods. The study employed contact and optical methods to analyze fabric and fiber surface characteristics, highlighting the significance of roughness profile parameters in understanding material changes post-treatment. The investigation revealed that mechanical action, alkaline medium, washing temperature, and detergent residues contribute to fabric erosion and modification during washing, with chitosan coatings creating pronounced surface irregularities. Comparative analysis showed significant fabric roughness changes post-washing, while fiber roughness changes were treatment specific. Despite initial increases in fiber roughness due to treatments, their durability decreased after washing. These findings emphasize the importance of roughness analysis in optimizing textile finishing processes and washing cycles, impacting both comfort and treatment efficacy.
Study on the Design and Mechanical Properties of a Novel Hexagonal Cell Body Topology
Authors: Hao Enze , Zhang Xindan , Zhao Xueqing , Zhang Hui
Published: August 2024

The honeycomb structure is a topological structure with excellent performance that stems from the properties of the basic units of the structure. Different structural features of basic units may lead to different mechanical characteristics in the whole part. In this study, a novel hexagonal cell body topology structure (NH) was designed and manufactured by the fused deposition modeling (FDM) technique to explore the effects on mechanical properties. The tensile and impact performance of the NH structure were compared with the regular hexagonal honeycomb structure (HH), and the influence of different unit single-cell sizes on the impact performance of the NH structure was investigated. The force transmission of the basic units of the NH structure was revealed through finite element analysis. The results indicate that both the tensile and impact performances of the NH structure have been improved compared to the HH structure. The improvement is due to the better force transmission capability of the basic units of the NH structure, leading to a more uniform stress distribution. Moreover, excessively large or small single-cell sizes of the NH structure will reduce the overall structure’s impact resistance. The overall structure achieves optimal impact resistance when the single-cell size is around 1.2 mm.
Advances in Hydrogel Polymers for Microbial Control in Water Systems
Authors: Akinsemolu Adenike A. , Onyeaka Helen
Published: August 2024

Every year, contaminated water is responsible for over one million deaths globally. Microbiology leads other fields in the development of solutions to water contamination to reduce these deaths while advancing the achievement of SDG 6, which aims to ensure universal access to water and sanitation. This article explores hydrogel polymers as a solution to water contamination through microbial control. Using a systematic approach, this study collects, reviews, analyzes, and synthesizes the findings of studies on the structure, properties, and mechanisms used by hydrogel polymers in pathogen control in water systems, emphasizing recent advances in microbiology that have improved the antimicrobial properties of hydrogel polymers, enhanced their synthetic properties, and improved their overall ability to control the spread of pathogens in water. Other additional notable findings, including the applications of hydrogel polymers in water systems, the environmental implications of using the method to decontaminate and purify water for various purposes, and the regulatory standards needed to reinforce the viability and effectiveness of the adaptation of hydrogel polymers for the control of harmful or unwanted microorganisms in water systems, inform the presented inferences on the future of hydrogel technologies and new opportunities for the expansion of their commercial use.
Biological Evaluation of Thermosensitive Hydrogels of Chitosan/Hydrolyzed Collagen/β-GP in an In Vitro Model of Induced Cardiac Ischemia
Authors: Marín Lina Orozco , Montoya Yuliet , Bustamante John
Published: August 2024

Ischemic events can culminate in acute myocardial infarction, which is generated by irreversible cardiac lesions that cannot be restored due to the limited regenerative capacity of the heart. Cardiac cell therapy aims to replace injured or necrotic cells with healthy and functional cells. Tissue engineering and cardiovascular regenerative medicine propose therapeutic alternatives using biomaterials that mimic the native extracellular environment and improve cellular and tissue functionality. This investigation evaluates the effect of thermosensitive hydrogels, and murine fetal ventricular cardiomyocytes encapsulated in thermosensitive hydrogels, on the contractile function of cardiomyocyte regeneration during an ischemic event. Chitosan and hydrolyzed collagen thermosensitive hydrogels were developed, and they were physically and chemically characterized. Likewise, their biocompatibility was evaluated through cytotoxicity assays by MTT, LDH, and their hemolytic capacity. The hydrogels, and cells inside the hydrogels, were used as an intervention for primary cardiomyocytes under hypoxic conditions to determine the restoration of the contractile capacity by measuring intracellular calcium levels and the expressions of binding proteins, such as a-actinin and connexin 43. These results evidence the potential of natural thermosensitive hydrogels to restore the bioelectrical functionality of ischemic cardiomyocytes.
Rapid Construction of PVA@CDs/SiO2 Fluorescent/Structural Color Dual-Mode Anti-Counterfeiting Labels via Spray-Coating Method
Authors: Liu Wenjuan , Yang Ling , Li Ting , Liu Yuejun , Tang Zengming , Hu Shuyang , Wang Xionggang , Tan Haihu
Published: August 2024

A method of sequential spraying of polyvinyl alcohol with carbon quantum dots (PVA@CDs) aqueous suspension and SiO2 aqueous suspension is proposed to rapidly prepare multicolor dual-mode anti-counterfeiting labels. With the optimization of the concentration (15%) of colloidal microspheres in the SiO2 aqueous suspension as well as the spraying process parameters (spray distance of 10 cm, spray duration of 3 s, and assembly temperature of 20 °C), different-sized SiO2 microspheres (168 nm, 228 nm, and 263 nm) were utilized to rapidly assemble red, green, and blue photonic crystals. Furthermore, the tunable fluorescence emission of carbon quantum dots endows the labels with yellow, green, and blue fluorescence. The constructed dual-mode labeling was used to develop an anti-counterfeiting code with dual-channel information storage capabilities and also to create dual-mode multicolor anti-counterfeiting labels on various packaging substrates. This work provides a novel solution for anti-counterfeiting packaging and information storage.
The Zhuangzi as a Commentary on Kongzi
Authors: Sellmann James Daryl
Published: August 2024

The role of Kongzi 孔子, in the Zhuangzi, has been a compelling story. Can we read the stories about Kongzi as constituting a type of commentary on his teachings and the early development of Confucian philosophy in general? First, let us consider the way Zhuangzi has put his own teachings into the mouth of Kongzi because he was accepted to be a sage who understood how to live well. Then, I turn to the more problematic references to Kongzi as being punished in Chapter 5 and his being a “condemned person” in chapter 6 to illustrate how these stories are taken to be forms of commentary or criticism of Kongzi’s project because he did not understand complete virtue. The role of Kongzi in the Zhuangzi can be read as a criticism-like commentary using Kongzi to criticize Kongzi.
A Two-Component Polarimetric Target Decomposition Algorithm with Grassland Application
Authors: Huang Pingping , Chen Yalan , Li Xiujuan , Tan Weixian , Chen Yuejuan , Yang Xiangli , Dong Yifan , Lv Xiaoqi , Li Baoyu
Published: August 2024

The study of the polarimetric target decomposition algorithm with physical scattering models has contributed to the development of the field of remote sensing because of its simple and clear physical meaning with a small computational effort. However, most of the volume scattering models in these algorithms are for forests or crops, and there is a lack of volume scattering models for grasslands. In order to improve the accuracy of the polarimetric target decomposition algorithm adapted to grassland data, in this paper, a novel volume scattering model is derived considering the characteristics of real grassland plant structure and combined with the backward scattering coefficients of grass, which is abstracted as a rotatable ellipsoid of variable shape. In the process of rotation, the possibility of rotation is considered in two dimensions, the tilt angle and canting angle; for particle shape, the anisotropy degree A is directly introduced as a parameter to describe and expand the applicability of the model at the same time. After obtaining the analytical solution of the parameters and using the principle of least negative power to determine the optimal solution of the model, the algorithm is validated by applying it to the C-band AirBorne dataset of Hunshandak grassland in Inner Mongolia and the X-band Cosmos-Skymed dataset of Xiwuqi grassland in Inner Mongolia. The performance of the algorithm with five polarimetric target decomposition algorithms is studied comparatively. The experimental results show that the algorithm proposed in this paper outperforms the other algorithms in terms of grassland decomposition accuracy on different bands of data.
The Typical ELF/VLF Electromagnetic Wave Activities in the Upper Ionosphere Recorded by the China Seismo-Electromagnetic Satellite
Authors: Hu Yunpeng , Zhima Zeren , Wang Tieyan , Lu Chao , Yang Dehe , Sun Xiaoying , Tang Tian , Cao Jinbin
Published: August 2024

Driven by the scientific objective of geophysical field detection and natural hazard monitoring from space, China launched an electromagnetic satellite, which is known as the China Seismo-Electromagnetic Satellite (CSES-01), on 2 February 2018, into a circular sun-synchronous orbit with an altitude of about 507 km in the ionosphere. The CSES-01 has been in orbit for over 6 years, successfully exceeding its designed 5-year lifespan, and will continually operate as long as possible. A second identical successor (CSES-02) will be launched in December 2024 in the same orbit space. The ionosphere is a highly dynamic and complicated system, and it is necessary to comprehensively understand the electromagnetic environment and the physical effects caused by various disturbance sources. The motivation of this report is to introduce the typical electromagnetic waves, mainly in the ELF/VLF band (i.e., ~100 Hz to 25 kHz), recorded by the CSES-01 in order to call the international community for deep research on EM wave activities and geophysical sphere coupling mechanisms. The wave spectral properties and the wave propagation parameters of those typical EM wave activities in the upper ionosphere are demonstrated in this study based on wave vector analysis using the singular value decomposition (SVD) method. The analysis shows that those typical and common natural EM waves in the upper ionosphere mainly include the ionospheric hiss and proton whistlers in the ELF band (below 1 kHz), the quasiperiodic (QP) emissions, magnetospheric line radiations (MLR), the falling-tone lightning whistlers, and V-shaped streaks in the ELF/VLF band (below 20 kHz). The typical artificial EM waves in the ELF/VLF band, such as power line harmonic radiation (PLHR) and radio waves in the VLF band, are also well recorded in the ionosphere.
Spatiotemporal Dynamics of Urban Green Space Coverage and Its Exposed Population under Rapid Urbanization in China
Authors: Zhai Chang , Geng Ruoxuan , Ren Zhibin , Wang Chengcong , Zhang Peng , Guo Yujie , Hong Shengyang , Hong Wenhai , Meng Fanyue , Fang Ning
Published: August 2024

Urban green spaces (UGSs) provide important support for the health of urban residents and the realization of sustainable urban development. However, the spatiotemporal pattern of urban resident exposure to UGSs in cities is unclear, especially at the national scale in China. Based on the annual 30 m resolution Normalized Difference Vegetation Index (NDVI) data of the Landsat satellite, we quantitatively analyzed the change in UGS coverage from 2000 to 2020 for 320 cities in China and combined it with population data to understand the changing patterns of urban population exposure to different UGS coverage. The results indicated that the average UGS coverage decreased from 63% to 44% from 2000 to 2020 in China, which could be divided into two stages: a rapid decline phase (2000–2014) and a progressive decline phase (2015–2020). Geographically, UGS coverage declined faster in southwestern and eastern cities than in other regions, particularly in medium-sized cities. We also found that urban pixel-based areas in cities with the highest UGS coverage (80–100%) decreased rapidly, and the proportion of the urban population exposed to the highest UGS coverage also declined significantly from 2000 to 2020. Urban pixel-based areas with low UGS coverage (20–40%) continued to expand, and there was a rapid increase in the proportion of the urban population exposed to low UGS coverage, with an increase of 146 million people from 2000 to 2020. The expansion of impervious surfaces had the most significant effect on the change in UGS coverage during different periods (2000–2020, 2000–2014, and 2015–2020). Natural factors such as precipitation, surface maximum temperature, and soil moisture also affected UGS coverage change. These findings provide insights into the impact of urbanization on the natural environment of cities, availability of UGS for residents, and sustainable urban development under rapid urbanization.