bluuebunny/crossref_metadata_2025 huggingface dataset:
Biomechanical comparison of subscapularis peel and lesser tuberosity osteotomy for double-row subscapularis repair technique in a cadaveric arthroplasty model
Authors: Virk Mandeep S. , Aiyash Saleh S. , Frank Rachel M. , Mellano Christopher S. , Shewman Elizabeth F. , Wang Vincent M. , Romeo Anthony A.
Published: November 2019

Introduction

Management of the subscapularis during shoulder arthroplasty is controversial. The purpose of this study was to compare the biomechanical performance of subscapularis peel (SP) and lesser tuberosity osteotomy (LTO) in a cadaveric model.

Methods

The subscapularis and proximal humerus were dissected from all soft tissues in 21 fresh-frozen human cadaveric shoulders and randomized to undergo SP, LTO, or standard subscapularis tenotomy (ST, control). For SP and LTO, six #5 sutures were passed through eyelets in the implant (on lateral border and through drill holes in bicipital groove [2] and under trunion [4]). Double-row repair was performed using two lateral row transosseous sutures and four medial row sutures through the tendon (SP) or osseotendinous junction (LTO). Biomechanical properties and mode of failure were tested.

Results

There were no significant differences in elongation amplitude, cyclic elongation, or maximum load to failure between the three groups (P > 0.05). Mean stiffness was significantly higher in LTO (P = 0.009 vs. SP and ST). In the ST group, 7/7 specimens failed at the tendon-suture interface. For SP, 4/7 failed at the tendon-suture interface, one at the suture-bone interface, one fractured around the implant stem, and one at the knots. For LTO, 3/7 failed at the tendon-suture interface, two at the suture-bone interface and two fractured around the implant stem.

Conclusions

In this cadaveric model, subscapularis repair via ST, SP, and LTO techniques was biomechanically equivalent. Additional studies are needed to confirm these findings and determine the influence of biologic healing on healing rates and clinical outcomes.

Level of evidence

N/a, biomechanical laboratory study
Morphological risk factors associated with dislocation after bipolar hemiarthroplasty of the hip in patients with femoral neck fractures—a nested case-control study
Authors: Zhang Yueqi , Yao Zhenjun , Shi Peng , Wang Chenzhong , Liu Jinyu , Yang Yi , Zhang Chi
Published: November 2019

Background

The relationship between preoperative hip measurements and dislocation after bipolar hemiarthroplasty is presently unclear. In the current study, we investigated the morphological risk factors associated with dislocation after bipolar hemiarthroplasty of the hip in patients with femoral neck fractures.

Methods

Between January 2011 and June 2017, a nested case-control design study was used to analyze the risk factors for dislocation in 348 patients who had undergone bipolar hemiarthroplasty because of femoral neck fractures. Twelve patients underwent at least one dislocation postoperatively. Sixty patients without dislocation were selected as controls matched in terms of time of surgery, age, and sex, at a ratio of 1:5. Patient acetabular measurements were compared between the dislocation group and the control group, including the center-edge angle, abduction angle, acetabular width and depth, depth-to-width ratio, femoral neck offset, leg length discrepancy, and femoral head coverage ratio. A multivariate logistic regression model was used to evaluate the morphological risk factors of dislocation.

Results

Postoperatively, the incidence of dislocation was 3.4%. A smaller center-edge angle was found to be a risk factor associated with dislocation after bipolar hemiarthroplasty of the hip. Patients with small acetabular depth and a small acetabular depth–width ratio were prone to dislocation. Patients with a center-edge angle of ≤ 45.4° or an acetabular depth of ≤ 19.12 mm were more likely to suffer dislocation.

Conclusions

Careful preoperative measurements before bipolar hemiarthroplasty of the hip are important. Surgical intervention for femoral neck fracture patients with a shallow acetabulum should be carefully planned and total hip arthroplasty should be considered when necessary.
Delivery strategies of cancer immunotherapy: recent advances and future perspectives
Authors: Zhao Zhongwei , Zheng Liyun , Chen Weiqian , Weng Wei , Song Jingjing , Ji Jiansong
Published: November 2019

Immunotherapy has become an emerging strategy for the treatment of cancer. Immunotherapeutic drugs have been increasing for clinical treatment. Despite significant advances in immunotherapy, the clinical application of immunotherapy for cancer patients has some challenges associated with safety and efficacy, including autoimmune reactions, cytokine release syndrome, and vascular leak syndrome. Novel strategies, particularly improved delivery strategies, including nanoparticles, scaffolds, and hydrogels, are able to effectively target tumors and/or immune cells of interest, increase the accumulation of immunotherapies within the lesion, and reduce off-target effects. Here, we briefly describe five major types of cancer immunotherapy, including their clinical status, strengths, and weaknesses. Then, we introduce novel delivery strategies, such as nanoparticle-based delivery of immunotherapy, implantable scaffolds, injectable biomaterials for immunotherapy, and matrix-binding molecular conjugates, which can improve the efficacy and safety of immunotherapies. Also, the limitations of novel delivery strategies and challenges of clinical translation are discussed.
Determinants of neonatal sepsis among neonates in the northwest part of Ethiopia: case-control study
Authors: Alemu Mulunesh , Ayana Mulatu , Abiy Hailemariam , Minuye Biniam , Alebachew Wubet , Endalamaw Aklilu
Published: November 2019

Background

Neonatal sepsis is one of the leading causes of neonatal morbidity and mortality. Despite implementing of different preventive interventions, the burden of neonatal sepsis is reporting in different areas of Ethiopia. For further interventions, identifying its determinants is found to be crucial.

Objective

This study aimed to identify determinants of neonatal sepsis in the Northwest part of Ethiopia.

Methods

Unmatched case-control study was conducted among 246 neonates admitted in neonatal intensive care unit, Northwest Ethiopia. Study participants were selected from February 1st to March 30th 2018. Data was collected through face to face interview and review of neonates’ medical records using pretested structured questionnaire. Data was entered into Epi Data version 4.2.0.0 and further transferred to SPSS statistical software version 25 for analysis. All independent variables withp-value < 0.25 in Bivariable analysis were entered into multivariable logistic regression analysis. Finally, variables with p-value < 0.05 were considered as determinants of neonatal sepsis.

Results

A total of 82 cases and 164 controls were included in this study. Neonates with gestational age < 37 weeks [AOR = 6.90; 95% CI (2.76, 17.28)], premature rupture of membrane [AOR = 2.81; 95% CI (1.01, 7.79)], not crying immediately at birth and have received resuscitation at birth [AOR = 2.85; 95% CI (1.09, 7.47)] were found to be predictors of neonatal sepsis.

Conclusions and recommendations

Premature rupture of membrane was found to be obstetric-related determinant of neonatal sepsis. Gestational age < 37 weeks, not crying immediately at birth, and have received resuscitation at birth were found to be neonatal-related risk factors of neonatal sepsis. Infection prevention strategies need to be strengthening and/or implementing by providing especial attention for the specified determinants.
First records of tick-borne pathogens in populations of the taiga tick Ixodes persulcatus in Sweden
Authors: Jaenson Thomas G. T. , Wilhelmsson Peter
Published: November 2019

Background

The common tickIxodes ricinusand the taiga tickI. persulcatusare the main tick vectors ofBorreliaspirochaetes, TBE virus (TBEV) and of several other zoonotic pathogens in the western and eastern areas, respectively of the Palaearctic region. Recently, populations of the taiga tick were, for the first time, detected in northern Sweden. This prompted us to investigate if they harbour human pathogens.

Methods

A total of 276I. persulcatusticks (136 males, 126 females and 14 nymphs) and oneI. ricinusnymph was collected by the cloth-dragging method in northern Sweden in July–August 2015 and May–July 2016. In addition, 8 males and 10 females ofI. persulcatuswere collected from two dogs (16 and 2 ticks, respectively) in two of the localities. All ticks were microscopically and molecularly identified to developmental stage and species and screened forB. burgdorferi(sensu lato),B. miyamotoi,Anaplasma phagocytophilum,Rickettsiaspp., Neoehrlichia mikurensis,Babesiaspp. and TBEV using real-time PCR followed by species identification by sequencing the PCR-products of conventional PCR assays.

Results

Of the ticks collected by the cloth-dragging method, 55% (152/277) were positive forBorrelia. There was no significant difference between the proportions ofBorrelia-infected nymphs (33%, 5/15) andBorrelia-infected adult ticks (56%, 147/262), and no significant difference between the proportions ofBorrelia-infected males (54%, 74/136) andBorrelia-infected females (58%, 73/126). Three differentBorreliaspecies were identified.Borrelia afzeliiwas the predominant species and detected in 46% of allBorrelia-infected ticks followed byB. garinii, 35%,B. valaisiana, 1%, and mixed infections of differentBorreliaspecies, 1%; 17% of allBorrelia-infections were untypeable. OneI. persulcatusfemale containedRickettsia helvetica, and one nymph containedRickettsiasp. Of the 277 ticks analysed, all were negative forA. phagocytophilum,Babesiaspp.,Borrelia miyamotoi,N. mikurensisand TBEV. The ticks collected from the two dogs were negative for all pathogens examined except forBorreliaspp., that was detected in 5 out of 16 ticks removed from one of the dogs.

Conclusions

To our knowledge, this is the first time thatI. persulcatusfrom Sweden has been analysed for the presence of tick-borne pathogens. The examined tick populations had a low diversity of tick-borne pathogens but a high prevalence ofB. burgdorferi(s.l.).
Combined genetic mutations and DNA-methylated genes as biomarkers for endometrial cancer detection from cervical scrapings
Authors: Liew Phui-Ly , Huang Rui-Lan , Wu Tzu-I , Liao Chi-Chun , Chen Chien-Wen , Su Po-Hsuan , Wang Hui-Chen , Weng Yu-Chun , Lai Hung-Cheng
Published: November 2019

Background

Endometrial cancer is a common gynecologic cancer. Noninvasive molecular biomarkers for triage of high-risk patients for invasive procedures are needed. Based on the success of cytological Pap smear screening, cervical scrapings are a good source of DNA for molecular testing. In addition to genetic lesions, DNA methylation is a promising biomarker. We assessed the usefulness of combining genetic and epigenetic biomarkers from cervical scrapings to detect endometrial carcinomas.

Methods

We performed a retrospective case–control study of 96 consecutive cervical scrapings from patients with abnormal uterine bleeding who underwent surgery for diagnostic evaluation. Thirty and 16 cases were diagnosed with type I and type II endometrial cancers, respectively. The remaining non-cancer cases included normal endometrium (n = 12), benign uterine lesions (n = 20), and endometrial hyperplasia (n = 18). Quantitative methylation-specific PCR and mass spectrometry were used for DNA methylation and genetic mutation analysis. Logistic regression was used to evaluate the clinical performance of these candidate biomarkers.

Results

We tested the effectiveness of the methylation status of four genes (BHLHE22, CDO1, TBX5, and HAND2) in endometrial cancer detection. The area under the receiver operating characteristic curves ranged from 0.703 to 0.878, and panels of hypermethylated BHLHE22/CDO1/HAND2 (87.0% sensitivity and 86.0% specificity) and BHLHE22/CDO1/TBX5 (89.1% sensitivity and 80.0% specificity) showed significant differences and could distinguish benign from malignant endometrial lesions. The sensitivity and specificity in endometrial cancer detection for BHLHE22/CDO1 were 84.8% and 88.0%, respectively. Both type I and II endometrial carcinomas could be detected using a BHLHE22/CDO1-based methylation profile, suggesting that they may have common epigenomes. Moreover, PTEN and TP53 mutations were found in 63.3% of type I and 93.6% of type II endometrial cancers. Unexpectedly, PTEN and TP53 mutations were commonly found in cervical scrapings of the normal endometrium (25% and 33.3%, respectively) and in cases with benign uterine lesions (10% and 50%, respectively). Finally, combinations of any one mutation of PTEN and TP53 mutations had a sensitivity of 91.3%, but a specificity of only 42.0%.

Conclusions

Adding PTEN/TP53 mutation testing to BHLHE22/CDO1-based methylation testing did not improve the detection of endometrial cancer.
Validation of an epigenetic field of susceptibility to detect significant prostate cancer from non-tumor biopsies
Authors: Yang Bing , Etheridge Tyler , McCormick Johnathon , Schultz Adam , Khemees Tariq A. , Damaschke Nathan , Leverson Glen , Woo Kaitlin , Sonn Geoffrey A. , Klein Eric A. , Fumo Mike , Huang Wei , Jarrard David F.
Published: November 2019

Background

An epigenetic field of cancer susceptibility exists for prostate cancer (PC) that gives rise to multifocal disease in the peripheral prostate. In previous work, genome-wide DNA methylation profiling identified altered regions in the normal prostate tissue of men with PC. In the current multicenter study, we examined the predictive strength of a panel of loci to detect cancer presence and grade in patients with negative biopsy tissue.

Results

Four centers contributed benign prostate biopsy tissues blocks from 129 subjects that were either tumor associated (TA, Grade Group [GG] ≥ 2, n = 77) or non-tumor associated (NTA, n = 52). Biopsies were analyzed using pyrosequencing for DNA methylation encompassing CpG loci near CAV1, EVX1, FGF1, NCR2, PLA2G16, and SPAG4 and methylation differences were detected within all gene regions (p < 0.05). A multiplex regression model for biomarker performance incorporating a gene combination discriminated TA from NTA tissues (area under the curve [AUC] 0.747, p = 0.004). A multiplex model incorporating all the above genes and clinical information (PSA, age) identified patients with GG ≥ 2 PC (AUC 0.815, p < 0.0001). In patients with cancer, increased variation in gene methylation levels occurs between biopsies across the prostate.

Conclusions

A widespread epigenetic field defect is utilized to detect GG ≥ 2 PC in patients with histologically negative biopsies. These alterations in non-tumor cells display increased heterogeneity of methylation extent and are spatially distant from tumor foci. These findings have the potential to decrease the need for repeated prostate biopsy.
Plasma levels of soluble TREM2 and neurofilament light chain in TREM2 rare variant carriers
Authors: Ashton Nicholas J. , Suárez-Calvet Marc , Heslegrave Amanda , Hye Abdul , Razquin Cristina , Pastor Pau , Sanchez-Valle Raquel , Molinuevo José L. , Visser Pieter Jelle , Blennow Kaj , Hodges Angela K. , Zetterberg Henrik
Published: November 2019

Background

Results from recent clinical studies suggest that cerebrospinal fluid (CSF) biomarkers that are indicative of Alzheimer’s disease (AD) can be replicated in blood, e.g. amyloid-beta peptides (Aβ42 and Aβ40) and neurofilament light chain (NFL). Such data proposes that blood is a rich source of potential biomarkers reflecting central nervous system pathophysiology and should be fully explored for biomarkers that show promise in CSF. Recently, soluble fragments of the triggering receptor expressed on myeloid cells 2 (sTREM2) protein in CSF have been reported to be increased in prodromal AD and also in individuals with TREM2 rare genetic variants that increase the likelihood of developing dementia.

Methods

In this study, we measured the levels of plasma sTREM2 and plasma NFL using the MesoScale Discovery and single molecule array platforms, respectively, in 48 confirmed TREM2 rare variant carriers and 49 non-carriers.

Results

Our results indicate that there are no changes in plasma sTREM2 and NFL concentrations between TREM2 rare variant carriers and non-carriers. Furthermore, plasma sTREM2 is not different between healthy controls, mild cognitive impairment (MCI) or AD.

Conclusion

Concentrations of plasma sTREM2 do not mimic the recent changes found in CSF sTREM2.
Osteogenic commitment of Wharton’s jelly mesenchymal stromal cells: mechanisms and implications for bioprocess development and clinical application
Authors: Cabrera-Pérez Raquel , Monguió-Tortajada Marta , Gámez-Valero Ana , Rojas-Márquez Raquel , Borràs Francesc Enric , Roura Santiago , Vives Joaquim
Published: November 2019

Background

Orthopaedic diseases are one of the major targets for regenerative medicine. In this context, Wharton’s jelly (WJ) is an alternative source to bone marrow (BM) for allogeneic transplantation since its isolation does not require an invasive procedure for cell collection and does not raise major ethical concerns. However, the osteogenic capacity of human WJ-derived multipotent mesenchymal stromal cells (MSC) remains unclear.

Methods

Here, we compared the baseline osteogenic potential of MSC from WJ and BM cell sources by cytological staining, quantitative real-time PCR and proteomic analysis, and assessed chemical and biological strategies for priming undifferentiated WJ-MSC. Concretely, different inhibitors/activators of the TGFβ1-BMP2 signalling pathway as well as the secretome of differentiating BM-MSC were tested.

Results

Cytochemical staining as well as gene expression and proteomic analysis revealed that osteogenic commitment was poor in WJ-MSC. However, stimulation of the BMP2 pathway with BMP2 plus tanshinone IIA and the addition of extracellular vesicles or protein-enriched preparations from differentiating BM-MSC enhanced WJ-MSC osteogenesis. Furthermore, greater outcome was obtained with the use of conditioned media from differentiating BM-MSC.

Conclusions

Altogether, our results point to the use of master banks of WJ-MSC as a valuable alternative to BM-MSC for orthopaedic conditions.
Arsenic metabolism in technical biogas plants: possible consequences for resident microbiota and downstream units
Authors: Weithmann Nicolas , Mlinar Stanislava , Hilbrig Frank , Bachmaf Samer , Arndt Julia , Planer-Friedrich Britta , Weig Alfons R. , Freitag Ruth
Published: November 2019

The metal(loid) and in particular the Arsenic (As) burden of thirteen agricultural biogas plants and two sewage sludge digesters were investigated together with the corresponding microbial consortia. The latter were characterized by ARISA (automated ribosomal intergenetic spacer analysis) and next generation sequencing. The consortia were found to cluster according to digester type rather than substrate or metal(loid) composition. For selected plants, individual As species in the liquid and gaseous phases were quantified, showing that the microorganisms actively metabolize and thereby remove the As from their environment via the formation of (methylated) volatile species. The As metabolites showed some dependency on the microbial consortia, while there was no statistical correlation with the substrate mix. Finally, slurry from one agricultural biogas plant and one sewage sludge digester was transferred into laboratory scale reactors (“satellite reactors”) and the response to a defined addition of As (30 and 60 µM sodium arsenite) was studied. The results corroborate the hypothesis of a rapid conversion of dissolved As species into volatile ones. Methanogenesis was reduced during that time, while there was no discernable toxic effect on the microbial population. However, the utilization of the produced biogas as replacement for natural gas, e.g. as fuel, may be problematic, as catalysts and machinery are known to suffer from prolonged exposure even to low As concentrations.
Ochratoxin A induces liver inflammation: involvement of intestinal microbiota
Authors: Wang Wence , Zhai Shuangshuang , Xia Yaoyao , Wang Hao , Ruan Dong , Zhou Ting , Zhu Yongwen , Zhang Hongfu , Zhang Minhong , Ye Hui , Ren Wenkai , Yang Lin
Published: November 2019

Background

Ochratoxin A (OTA) is a widespread mycotoxin and induces liver inflammation to human and various species of animals. The intestinal microbiota has critical importance in liver inflammation; however, it remains to know whether intestinal microbiota mediates the liver inflammation induced by OTA. Here, we treated ducklings with oral gavage of OTA (235 μg/kg body weight) for 2 weeks. Then, the microbiota in the cecum and liver were analyzed with 16S rRNA sequencing, and the inflammation in the liver was analyzed. To explore the role of intestinal microbiota in OTA-induced liver inflammation, intestinal microbiota was cleared with antibiotics and fecal microbiota transplantation was conducted.

Results

Here, we find that OTA treatment in ducks altered the intestinal microbiota composition and structure [e.g., increasing the relative abundance of lipopolysaccharides (LPS)-producing Bacteroides], and induced the accumulation of LPS and inflammation in the liver. Intriguingly, in antibiotic-treated ducks, OTA failed to induce these alterations in the liver. Notably, with the fecal microbiota transplantation (FMT) program, in which ducks were colonized with intestinal microbiota from control or OTA-treated ducks, we elucidated the involvement of intestinal microbiota, especially Bacteroides, in liver inflammation induced by OTA.

Conclusions

These results highlight the role of gut microbiota in OTA-induced liver inflammation and open a new window for novel preventative or therapeutic intervention for mycotoxicosis.
The influence of a single species on the space use of mixed-species flocks in Amazonian Peru
Authors: Williams Sean M. , Lindell Catherine A.
Published: November 2019

Background

The drivers of space use patterns of multi-species groups have been poorly studied, although mixed-species avian flocks are common throughout the world. In a mixed-species flock, multiple species move together and maintain proximity. The different species may or may not have conflicting preferences of space use. We hypothesized that the space use patterns of the flock are driven by a single species.

Methods

We investigated the behavioral drivers of space use patterns of mixed-species flocks in Amazonian Peru by mapping 95% fixed-kernel home ranges of three flocks, which then we divided into high-use (inner 55% kernel utilization distribution) and low-use areas (lying outside the high-use area). We quantified the foraging and anti-predator behavior of individual birds in the flocks. We tested whether foraging and anti-predator behavior of different species were different in high use and low use areas of the flock.

Results

We collected 455 spatial points and 329 foraging and anti-predator behavior observations on three flocks. The single best model for explaining the space use patterns of the flocks contained only vegetation density that surrounded Dusky-throated Antshrikes.

Conclusion

The results are consistent with the hypothesis that a single species in mixed-species flocks has a disproportionately large influence on space use patterns. The surrounding vegetation density of the Dusky-throated Antshrike was the only driver of space use patterns of flocks supported by our data. The results may apply to flocks pantropically, many of which are led by species that behave similarly to the Dusky-throated Antshrike, e.g. Asian flocks led by drongos (Dicrurus spp.).
Development and validation of a liquid chromatography-tandem mass spectrometric method for the determination of lenalidomide in human plasma and its application on bioequivalence studies
Authors: Gopinath R. , Narenderan S. T. , Kumar M. , Babu B.
Published: November 2019

A simple, sensitive, and specific liquid chromatography-tandem mass spectrophotometry (LC-MS/MS) method was developed and validated for the quantification of lenalidomide in human plasma. The separation was carried out on a symmetry, C18, 5-μm (50 × 4.6 mm) column as stationary phase and with an isocratic mobile phase of 0.1% formic acid in water-methanol in the ratio of (15:85, v/v) at a flow rate of 0.5 mL/min. Protonated ions formed by electrospray ionization in the positive mode were used to detect analyte and fluconazole (internal standard). The mass detection was made by monitoring the fragmentation of m/z 260.1/148.8 for lenalidomide and m/z 307.1/238.0 for internal standard on a triple quadrupole mass spectrometer. The developed method was validated over the concentration range of 10–1000 ng/mL for lenalidomide in human plasma with a correlation coefficient (r2) was 0.9930. The accuracy and precision values obtained from six different sets of quality control samples analyzed on separate occasions ranged from 99.41 to 106.97% and 2.88 to 4.22%, respectively. Mean extraction recoveries were 98.06% and 88.78% for the analyte and IS, respectively. The developed method was successfully applied for analyzing lenalidomide in human plasma samples.
Evaluating a novel sign’s impact on whether park visitors enter a dangerous river
Authors: Girasek Deborah C.
Published: November 2019

Background

Between 1972 and 2015, 56 visitors to the two national parks that border the Potomac River Gorge experienced fatal drowning. In 2016, the George Washington Memorial Parkway (GWMP), and the Chesapeake and Ohio National Historical Park (CHOH) partnered with a researcher to see if enhancement of their risk communication strategies could reduce behaviors that contribute to these deaths.

Methods

An experimental sign, which informed visitors that water entry was illegal and could result in a fine exceeding $200 was developed, and displayed on alternating weekend days from July 30 to September 11, 2016. Those signs were displayed at each park’s entrance, on restroom doors, at trailheads, and at both shorelines of the Potomac. At other times the experimental signs were covered, but a standard safety sign was always present. Cameras were used to record water entries.

Results

Cameras captured 1441 images. Approximately 2% of the images in CHOH and 1% of the images in GWMP showed a visitor in the water. Our multivariate analysis revealed that air temperature, beach count, and sign condition were significantly associated with water entry. When our experimental sign was displayed, the odds of an image showing someone in the water was reduced by 63%.

Conclusions

A sign alerting park visitors to the fact that water entry is illegal, and could potentially result in a considerable fine, was associated with significantly reduced risk-taking. While intuitive, this finding is a reminder to consider whether warnings that focus on non-health consequences might be more salient to at-risk populations.
A review of challenges and solutions in the preparation and use of magnetorheological fluids
Authors: Kumar James Sathya , Paul P. Sam , Raghunathan Girish , Alex Divin George
Published: November 2019

This review of MRF (magnetorheological fluids or MR fluids) brings out the challenges in methods of preparation, difficulties encountered in storage and use, and possible solutions to overcome the challenges.

Magnetorheological fluid in the rheological fluid domain has found use due to its ability to change its shear strength based on the applied magnetic field. Magnetorheological fluids are composed of magnetizable micron-sized iron particles and a non-magnetizable base or carrier fluid along with additives to counter sedimentation and agglomeration.

Magnetorheological fluids can respond to external stimuli by undergoing changes in physical properties thus enabling several improved modifications in the existing technology enhancing their application versatility and utility. Thus, magnetorheological fluid, a rheological material whose viscosity undergoes apparent changes on application of magnetic field, is considered as a smart material. Such materials can be used for active and semi-active control of engineering systems.

Many studies on the designs of systems incorporating MR fluids, mainly for vibration control and also for other applications including brakes, clutches, dynamometers, aircraft landing gears, and helicopter lag dampers, have emerged over last couple of decades. However, the preparation as well as the maintenance of magnetorheological fluids involves several challenges. Sedimentation is a major challenge, even when stored for moderate periods of time. A comprehensive review is made on the problems confronted in the preparation of magnetorheological fluids as well as sustenance of the properties, for use, over a long period of time. Other problems encountered include agglomeration and in-use thickening (IUT) as well as rusting and crusting. Of interest is the mitigation of these problems so as to prepare fluids with satisfactory properties, and such solutions are reviewed here. The control of magnetorheological fluids and the applications of interest are also reviewed.

The review covers additives for overcoming challenges in the preparation and use of magnetorheological fluids that include incrustation, sedimentation, agglomeration, and also oxidation of the particles. The methodology to prepare the fluid along with the process for adding selected additives was reviewed. The results showed an improvement in the reduction of sedimentation and other problems decreasing comparatively. A set of additives for addressing the specific challenges has been summarized. Experiments were carried out to establish the sedimentation rates for compositions with varying fractions of additives.

The review also analyzes briefly the gaps in studies on MR fluids and covers present developments and future application areas such as haptic devices.
Filiform polyposis with sigmoid colon adenocarcinoma: a case report
Authors: Okuno Takayuki , Kanazawa Takamitsu , Kishi Hirohisa , Anzai Hiroyuki , Yasuda Koji , Ishihara Soichiro
Published: November 2019

Background

Filiform polyposis is a rare form of inflammatory polyposis, which is occasionally formed in the colon of patients with history of inflammatory bowel disease (IBD). It is characterized by presence of several to hundreds of slender, worm-like polyps in the colon lined by histologically normal colonic mucosa and often coalesce, resulting in a tumor-like mass. Filiform polyposis is most frequently associated with a post-inflammatory reparative process in patients with IBD history, and only cases of filiform polyposis occurring in patients without IBD history have been reported. Filiform polyposis has been considered as a benign inflammatory polyposis without any risk of dysplasia, while the possibility of carcinogenesis of inflammatory polyps is not fully excluded. To date, only three cases of filiform polyposis coexisting with dysplasia have been reported.

Case presentation

A 59-year-old male patient with no past medical history of IBD underwent laparoscopic sigmoidectomy for obstructive filiform polyposis, which was associated with sigmoid colon adenocarcinoma. Based on the histological findings of the resected specimen, invasive sigmoid colon adenocarcinoma was surrounded by filiform polyposis, and adenocarcinoma also scattered uniformly on the surface of filiform polyposis. In immunohistochemistry, abnormal p53 expression was observed in adenocarcinoma, while it was not shown in mucosa on filiform polyposis.

Conclusions

This is the fourth case of filiform polyposis that is closely associated with colon dysplasia or adenocarcinoma based on histological findings. However, immunohistochemical findings did not support the theory that inflammation initiates adenocarcinoma in filiform polyposis like IBD. Hence, further immunohistochemical and genetic analyses are needed to clarify the association between filiform polyposis and carcinogenesis.
Patients’ and researchers’ experiences with a patient board for a clinical trial on urinary tract infections
Authors: Schilling Imke , Behrens Heike , Bleidorn Jutta , Gágyor Ildikó , Hugenschmidt Claudia , Jilani Hannah , Schmiemann Guido , Gerhardus Ansgar
Published: November 2019

Background

Patient and public involvement (PPI) has become an essential part of the design, conduct, and dissemination of research. While researchers who employed PPI mainly report on the positive aspects, in practice PPI is still an exception in clinical trials in Germany. There are specific challenges in the process of involvement that can jeopardize the conduct of involvement. The aim of our study was to analyze the experience of patients and researchers with PPI in a clinical trial in Germany, so we could learn more about potential challenges and how they could be addressed.

Methods

We established a patient board for a randomized controlled trial on urinary tract infections, where patients and researchers regularly met to discuss relevant aspects of the trial. Minutes were taken for each meeting and the moderator also noted her observations in a postscript. After four meetings, we conducted two focus groups, one each with the patients and researchers. We analyzed and categorized the minutes, postscripts, and focus group transcripts using thematic qualitative text analysis.

Results

Patients and researchers felt comfortable with the composition of the patient board and its’ atmosphere. In terms of challenges, patients and researchers needed time to get familiar with PPI. Both parties saw a need for training in PPI but differed in their views on the relevant topics. Patients wished to learn more about their role and tasks within the board at the onset of the PPI. They also preferred to meet more frequently and get more intensely involved in the trial. In contrast, researchers perceived that they were already highly involved. They further felt that the involvement was of benefit to them, the trial and future research. Patients described benefits for themselves, but also wondered if their involvement had had an impact on the trial.

Conclusions

To facilitate effective PPI, resources, adequate structures, and training are needed. Patients and researchers need to agree on their respective roles, training needs, and the mode of cooperation right at the beginning. The parties involved should continuously reflect on the actual benefits of PPI, describe them explicitly and make them transparent for all.
Correction to: Characterization of the left ventricular arrhythmogenic substrate with multimodality imaging: role of innervation imaging and left ventricular global longitudinal strain
Authors: El Mahdiui Mohammed , Smit Jeff M. , van Rosendael Alexander R. , Delgado Victoria , Marsan Nina Ajmone , Jukema J. Wouter , Scholte Arthur J. H. A. , Bax Jeroen J.
Published: November 2019

The original publication of this article El Mahdiui et al., 2019 contained 2 errors that could not be updated prior to publication. The correct and incorrect information is shown below for clarification. The changed information is shown in bold. These changes do not affect the interpretation and conclusion of the article.
Optimization of some fermentation conditions for bioethanol production from microalgae using response surface method
Authors: El-Mekkawi Samar A. , Abdo Sayeda M. , Samhan Farag A. , Ali Gamila H.
Published: November 2019

Background

Algal biomass fermentation is one of the promising alternatives for bioethanol production. The bioethanol yield relies on fermentation conditions as the algal biomass amount, the yeast volume (% v/v), and the fermentation time. In this work, algal biomass harvested from a pilot-scale high rate algal pond (HRAP) was fermented anaerobically using immobilizedSaccharomyces cerevisiae(ATCC 4126). The HRAP was constructed at the Zenin wastewater treatment plant (WTP), Giza, Egypt. A separate hydrolysis fermentation process (SHF) was applied for algal biomass. The effect of the algal biomass amount, the yeast volume (% v/v), and the time of fermentation as three independent variables were studied simultaneously and analyzed statistically using Design-Expert software V6.0.8.

Results

The harvested algal biomass from HRAP contains 45% carbohydrates and was dominated byMicrocystissp.The results revealed that optimum bioethanol yield 18.57 g/L is achieved by fermenting 98.7 g/L algae using 15.09% of the volume immobilized yeast for 43.6 h with a 95% confidence interval.

Conclusion

Microalgae grown on wastewater are a promising source of bioethanol production. Maximizing the ethanol production is achieved by optimizing the fermentation parameters as algal biomass, fermentation time, and yeast volume percent. The simultaneous optimization of the parameters using a statistical program is an effective way to maximize the production and predict a model that describes the relationship between these parameters and their response. The prospective research is going to study the effect of these predicted parameters on continuous fermentation on the semi-pilot scale.
New directions in socioscientific issues research
Authors: Zeidler Dana L. , Herman Benjamin C. , Sadler Troy D.
Published: November 2019

The socioscientific issues framework has proven to have a significant impact over the last two decades on many areas related to the development of functional scientific literacy in students. In this article, we summarize and synthesize recent trends in socioscientific issues research that impact both disciplinary and interdisciplinary science education research. These trends represent science-in-context investigations that we propose are advanced by three broad and interrelated areas of research including: 1) Socioscientific Issues and the Central Role of Socioscientific Reasoning; 2) Socioscientific Issues and the Primacy of Socioscientific Perspective Taking; and, 3) Socioscientific Issues and the Importance of Informal and Place-Based Contexts. We discuss the most recent research in those areas and explore the educational significance these new trends.
MR diffusion tensor imaging of the spinal cord: can it help in early detection of cervical spondylotic myelopathy and assessment of its severity?
Authors: Hassan Talaat Ahmed Abd El Hameed , Assad Ramy Edward , Belal Shaimaa Atef
Published: November 2019

Background

The aim of this study is to evaluate the potential application of MR diffusion tensor imaging (with calculation of fractional anisotropy (FA) values) in assessment of the spondylotic cervical spinal canal compromise and comparison with the information issued from conventional MR sequences for early detection of cervical spondylotic myelopathy (CSM). Thirty patients (11 males and 19 females) were included in this study; age ranged from 22 to 70 years (mean age = 44). All patients had conventional and diffusion tensor imaging (DTI) examinations of the cervical spine for detection and assessment of degree of cervical cord myelopathy. FA values of the whole cord circumference and at 3, 6, 9, 12 o’clock positions of the normal cord (opposite to C2), opposite to the most affected disc, and below the level of the most affected disc were measured.

Results

High statistically significantPvalues were obtained when comparing the FA values of the normal cord with the cord opposite to the most affected disc, the normal cord with the cord below the affected disc and the cord at the level of the most affected disc with the cord below the level of the most affected disc.

Conclusions

DTI of the cervical spinal cord with FA measurement in patients with cervical spondylosis helps in early detection of cervical cord compressive myelopathy prior to appearance of changes in conventional MRI, which can improve the clinical outcome and help in treatment plans.
Effect of External Electric Fields on the Oxidation Reaction of Olefins by Fe(IV)OCl–Porphyrin Complexes
Authors: Ma Zhifeng , Nakatani Naoki , Fujii Hiroshi , Hada Masahiko
Published: November 2019

Insights of environmental perturbation (an external electric field, EEF) are complicated but important in terms of experiments. In this paper, we report theoretical results of the effect of an EEF on olefin epoxidation by an Fe(IV)OCl–porphyrin complex using density functional theory. The EEF along the electron flow greatly affects the potential energy profile, and thereby affects the reaction mechanism and stabilization of the species. The results show that a negative EEF catalyzes ethylene epoxidation, whereas a positive EEF inhibits the reaction. Moreover, an EEF can exchange the ground state with the low-lying excited states. Therefore, the potential energy profile along the epoxidation reaction is mainly modified by the electron transfer from ethylene to the Fe(IV)OCl–porphyrin complex.
Catalytic Synthesis of Isoquinolines via Intramolecular Migration of N-Aryl Sulfonyl Groups on 1,5-Yne-Imines
Authors: Hoshimoto Yoichi , Nishimura Chika , Sasaoka Yukari , Kumar Ravindra , Ogoshi Sensuke
Published: November 2019

Isoquinolines are ubiquitous structural motifs in a variety of bioactive compounds, including medicinal agents and natural products. The development of novel strategies for the preparation of isoquinolines using non-toxic organocatalysts is thus worthwhile. Herein, we report a simple amine-catalyzed protocol for the synthesis of isoquinolines from 1,5-yne-imines via the intramoleular migration of an N-aryl sulfonyl group to the carbon atom of the alkyne moiety.
Retracted: An application study of CT perfusion imaging in assessing metastatic involvement of perigastric lymph nodes in patients with T1 gastric cancer
Authors: Sun Zongqiong , Hu Shudong , Li Jie , Wang Teng , Xie Zhihui , Jin Linfang
Published: November 2019

Objective:

To assess metastatic involvement of perigastric lymph nodes (PLNs) in patients with T1 gastric cancer by using CT perfusion imaging (CTPI).

Methods:

A total of 82 annotated PLNs of 33 patients with T1 gastric cancer confirmed by endoscopic ultrasonography underwent CTPI and portal phase CT scan before operation. The scan data were post-processed to acquire perfusion maps and calculate perfusion parameters including blood flow (BF) and permeability surface (PS). A radiologist measured the short axis diameters and perfusion parameters of PLNs. According to the post-operative pathology result, PLNs were divided into two groups: metastatic and inflammatory LNs. Perfusion parameters values and the size of PLNs between two groups were respectively compared statistically by t-test, and a receiver operating characteristic curve analysis was used to determine the optimal diagnostic cut-off value with sensitivity, specificity and area under the curve.

Results:

Examined 82 PLNs were metastatic in 45 (54.9%) and inflammatory in 37 (45.1%). The mean values of perfusion parameters and the short axis diameters in metastatic and inflammatory PLNs, respectively, were BF of 97.48 vs 81.21 ml/100 mg /min (p < 0.001), PS of 45.11 vs 36.80 ml/100 mg /min (p < 0.001), and the size of 1.51 cm vs 1.29 cm (p = 0.059). The sensitivity of 84.4%, specificity of 67.6% and area under the curve of 0.826 for BF with cut-off value of 88.89 ml/100 mg /min for differentiating metastatic from inflammatory nodes were higher than those of PS or the size of PLNs (p < 0.001).

Conclusion:

CT perfusion parameters values were different between metastatic and inflammatory PLNs in T1 gastric cancer. BF value may be the most reliable diagnostic marker of metastatic PLNs, and it is helpful for clinicians to choose treatment modality or management plan in T1 gastric cancer patients.

Advances in knowledge:

CTPI gives information on vascularization of LNs.

BF value might be a more effective marker than PS or the size of LNs for differentiating metastatic from inflammatory LNs in patients with T1 gastric cancer.
Reliability and accuracy of segmentation of mandibular condyles from different three-dimensional imaging modalities: a systematic review
Authors: Kim Justin J. , Nam Hyejin , Kaipatur Neelambar R. , Major Paul W. , Flores-Mir Carlos , Lagravere Manuel O. , Romanyk Daniel L.
Published: November 2019

Objective:

To critically synthesize the literature surrounding segmentation of the mandibular condyle using three-dimensional imaging modalities. Specifically, analyzing the reliability and accuracy of methods used for three-dimensional condyle segmentation.

Methods:

Three electronic databases were searched for studies reporting the reliability and accuracy of various methods used to segment mandibular condyles from three-dimensional imaging modalities. Two authors independently reviewed articles for eligibility and data extraction.

Results:

Nine studies fulfilled the inclusion criteria. Eight studies assessed the condylar segmentation from CBCT images and limited studies were available on non-CBCT three-dimensional imaging modalities. Threshold-based volume segmentation, manual segmentation, and semi-automatic segmentation techniques were presented. Threshold-based volume segmentation reported higher accuracy when completed by an experienced technician compared to clinicians. Adequate reliability and accuracy were observed in manual segmentation. Although adequate reliability was reported in semi-automatic segmentation, data on its accuracy were lacking.

Conclusion:

A definitive conclusion with regards to which current technique is most reliable and accurate to efficiently segment the mandibular condyle cannot be made with the currently available evidence. This is especially true in terms of non-CBCT imaging modalities with very limited literature available.
Changes in Body Composition, Energy Metabolism, and Appetite-Regulating Hormones in Korean Professional Female Ballet Dancers Before and After Ballet Performance
Authors: Kim Soo Youn , Cho Jung Ho , Lee Ji Hyun , Jung Jae Hyun
Published: November 2019

This study investigated changes in body composition, energy balance, and appetite-regulating hormones in professional female ballet dancers before and after 3 days of ballet performances. The subjects were 43 professional female ballet dancers in Korea. The mean age of the subjects
was 25.9 ± 2.8 years, and they had over 13 years of ballet training on average. For body composition, the body mass index (BMI), percent body fat (%BF), lean body mass (LBM), and total body water (TBW) were evaluated. By way of blood analysis the serum levels of ghrelin, leptin, and
insulin were examined. The calculations of energy intake (EI) and expenditure (EE) were based on journals that were self-recorded by the subjects for 14 days. For statistical analysis, the dependent sample t-test was applied (p < 0.05). The results showed no significant change in %BF, but
the BMI, LBM, and TBW increased significantly in the post-performance measurement. Energy balance results demonstrated a significant increase in EI and decrease in EE. Both the ghrelin and leptin levels increased significantly. Although reported energy intake increased after performances,
it remained below estimated energy requirements. Ballet dancers should be aware of the need to maintain energy balance in order to optimize their health and performance.
On the explicit use of experimental images in high resolution cryo-EM reﬁnement
Authors: Cherfils Jacqueline , Navaza Jorge
Published: November 2019

Single particle cryogenic electron microscopy (cryo-EM) is transforming structural biology by enabling the analysis of difficult macromolecular specimens, such as membrane proteins or large complexes with flexible elements, at near atomic resolution with an accuracy close to that of X-ray crystallography. As the technique continues to improve, it is important to assess and exploit its full potential to produce the most possible reliable atomic models. Here we propose to use the experimental images as the data for refinement and validation, instead of the reconstructed maps as currently used. This procedure, which is in spirit quite similar to that used in X-ray crystallography where the data include experimental phases, should contribute to improve the quality of the cryo-EM atomic models.