bluuebunny/crossref_metadata_2025 huggingface dataset:
Waste Orange Peels as a Source of Cellulose Nanocrystals and Their Use for the Development of Nanocomposite Films
Authors: Bigi Francesco , Maurizzi Enrico , Haghighi Hossein , Siesler Heinz , Licciardello Fabio , Pulvirenti Andrea
Published: February 2023

To date, approximately 30–50% of food is wasted from post-harvesting to consumer usage. Typical examples of food by-products are fruit peels and pomace, seeds, and others. A large part of these matrices is still discarded in landfills, while a small portion is valorized for bioprocessing. In this context, a feasible strategy to valorize food by-products consists of their use for the production of bioactive compounds and nanofillers, which can be further used to functionalize biobased packaging materials. The focus of this research was to create an efficient methodology for the extraction of cellulose from leftover orange peel after juice processing and for its conversion into cellulose nanocrystals (CNCs) for use in bionanocomposite films for packaging materials. Orange CNCs were characterized by TEM and XRD analyses and added as reinforcing agents into chitosan/hydroxypropyl methylcellulose (CS/HPMC) films enriched with lauroyl arginate ethyl (LAE®). It was evaluated how CNCs and LAE® affected the technical and functional characteristics of CS/HPMC films. CNCs revealed needle-like shapes with an aspect ratio of 12.5, and average length and width of 500 nm and 40 nm, respectively. Scanning electron microscopy and infrared spectroscopy confirmed the high compatibility of the CS/HPMC blend with CNCs and LAE®. The inclusion of CNCs increased the films’ tensile strength, light barrier, and water vapor barrier properties while reducing their water solubility. The addition of LAE® improved the films’ flexibility and gave them biocidal efficacy against the main bacterial pathogens that cause foodborne illness, such as Escherichia coli, Pseudomonas fluorescens, Listeria monocytogenes, and Salmonella enterica.
Deterrence, Backup, or Insurance: Game-Theoretic Modeling of Ransomware
Authors: Yin Tongxin , Sarabi Armin , Liu Mingyan
Published: February 2023

In this paper, we present a game-theoretic analysis of ransomware. To this end, we provide theoretical and empirical analysis of a two-player Attacker-Defender (A-D) game, as well as a Defender-Insurer (D-I) game; in the latter, the attacker is assumed to be a non-strategic third party. Our model assumes that the defender can invest in two types of protection against ransomware attacks: (1) general protection through a deterrence effort, making attacks less likely to succeed, and (2) a backup effort serving the purpose of recourse, allowing the defender to recover from successful attacks. The attacker then decides on a ransom amount in the event of a successful attack, with the defender choosing to pay ransom immediately, or to try to recover their data first while bearing a recovery cost for this recovery attempt. Note that recovery is not guaranteed to be successful, which may eventually lead to the defender paying the demanded ransom. Our analysis of the A-D game shows that the equilibrium falls into one of three scenarios: (1) the defender will pay the ransom immediately without having invested any effort in backup, (2) the defender will pay the ransom while leveraging backups as a credible threat to force a lower ransom demand, and (3) the defender will try to recover data, only paying the ransom when recovery fails. We observe that the backup effort will be entirely abandoned when recovery is too expensive, leading to the (worst-case) first scenario which rules out recovery. Furthermore, our analysis of the D-I game suggests that the introduction of insurance leads to moral hazard as expected, with the defender reducing their efforts; less obvious is the interesting observation that this reduction is mostly in their backup effort.
Prenatal Detection of a FOXF1 Deletion in a Fetus with ACDMPV and Hydronephrosis
Authors: Bzdęga Katarzyna , Kutkowska-Kaźmierczak Anna , Deutsch Gail H. , Plaskota Izabela , Smyk Marta , Niemiec Magdalena , Barczyk Artur , Obersztyn Ewa , Modzelewski Jan , Lipska Iwona , Stankiewicz Paweł , Gajecka Marzena , Rydzanicz Małgorzata , Płoski Rafał , Szczapa Tomasz , Karolak Justyna A.
Published: February 2023

Alveolar capillary dysplasia with misalignment of pulmonary veins (ACDMPV) is a lethal lung developmental disorder caused by the arrest of fetal lung formation, resulting in neonatal death due to acute respiratory failure and pulmonary arterial hypertension. Heterozygous single-nucleotide variants or copy-number variant (CNV) deletions involving the FOXF1 gene and/or its lung-specific enhancer are found in the vast majority of ACDMPV patients. ACDMPV is often accompanied by extrapulmonary malformations, including the gastrointestinal, cardiac, or genitourinary systems. Thus far, most of the described ACDMPV patients have been diagnosed post mortem, based on histologic evaluation of the lung tissue and/or genetic testing. Here, we report a case of a prenatally detected de novo CNV deletion (~0.74 Mb) involving the FOXF1 gene in a fetus with ACDMPV and hydronephrosis. Since ACDMPV is challenging to detect by ultrasound examination, the more widespread implementation of prenatal genetic testing can facilitate early diagnosis, improve appropriate genetic counselling, and further management.
IAG Regulates the Expression of Cytoskeletal Protein-Encoding Genes in Shrimp Testis
Authors: Lv Qian , Li Shihao , Miao Miao , Jin Songjun , Li Fuhua
Published: February 2023

Insulin-like androgenic gland hormone (IAG) is the master regulator of sexual differentiation and testis development in male crustaceans. However, the molecular mechanism on how IAG functions during testis development is still largely unknown. Here, the transcriptional changes were analyzed in the testes of shrimp after LvIAG knockdown in Litopenaeus vannamei. Differential expression analysis identified 111 differentially expressed genes (DEGs), including 48 upregulated DEGs and 63 downregulated DEGs, in testes of shrimp after LvIAG knockdown. Gene ontology (GO) analysis showed that these DEGs were apparently enriched in cytoskeleton-related GO items. Gene function analysis showed that genes enriched in these GO items mainly encoded actin, myosin, and heat shock protein. Interestingly, these genes were all downregulated in testis after LvIAG knockdown, which was confirmed by qRT-PCR detection. Furthermore, injection of LvIAG protein that was recombinantly expressed in insect cells upregulated the expression levels of these genes. The present study revealed that shrimp IAG might function in testis development through regulating the expression of cytoskeletal protein-encoding genes, which would provide new insights into understanding the functional mechanisms of IAG on male sexual development of crustaceans.
Exposure to Juvenile Stress Induces Epigenetic Alterations in the GABAergic System in Rats
Authors: Ben David Gil , Amir Yam , Tripathi Kuldeep , Sharvit Lital , Benhos Amir , Anunu Rachel , Richter-Levin Gal , Atzmon Gil
Published: February 2023

Epigenetics is a gene–environment interaction mechanism, manifested mostly through changes in regulatory gene expression. Stress is an established environmental factor known to induce epigenetic changes. This study aimed to assess the long-term effect of stress as juveniles, or juvenile and adult stress, on alterations in glutamic acid decarboxylase genes (GAD65, GAD67). We assessed DNA methylation and RNA expression in four rat groups: (1) control group, (2) juvenile stress group sacrificed two days following stress exposure (JSe) (RNA only), (3) juvenile stress group sacrificed as adults (JS), and (4) juvenile and adult stress group (JS + AS). Three different areas of the brain were examined in each group: the dorsal dentate gyrus (dDG), the dorsal CA1 (dCA1), and the basolateral amygdala (BLA). A significantly low methylation level of GAD65 in the BLA was observed among the JS group, followed by almost complete recovery among the JS + AS group. However, in dDG, an opposite trend was captured, and higher GAD65 methylation was found in JS. In addition, RNA levels were found to be decreased in JS compared to JSe and JS + AS. These findings can point to a possible mechanism: while juvenile stress may enhance a better coping strategy with life challenges, additional stress in adulthood may trigger a contradictory response, either beneficial or harmful.
Anxiety and Depression in the Initial Stage of the COVID-19 Outbreak in a Portuguese Sample: Exploratory Study
Authors: José Helena , Oliveira Cláudia , Costa Emília , Matos Filomena , Pacheco Eusébio , Nave Filipe , Valentim Olga , Sousa Luís
Published: February 2023

Background: In previous studies, it was found that the confinement to which the population was subjected during the quarantine of the COVID-19 pandemic increased the risk of anxiety and depression. Objective: to analyze the levels of anxiety and depression symptoms in Portugal residents during the quarantine of the COVID-19 pandemic. Methods: This is a descriptive, transversal, and exploratory study of non-probabilistic sampling. Data collection was carried out between 6th and 31st of May 2020. Sociodemographic and health questionnaires PHQ-9 and GAD-7 were used. Results: The sample consisted of 920 individuals. The prevalence for depressive symptoms (PHQ-9 ≥ 5) was 68.2% and (PHQ-9 ≥ 10) was 34.8%, and for anxiety symptoms (GAD-7 ≥ 5) was 60.4% and (GAD-7 ≥ 10) was 20%. Depressive symptoms were moderately severe for 8.9% of the individuals, and 4.8% presented severe depression. Regarding the generalized anxiety disorder, we found that 11.6% of individuals present moderate symptoms, and 8.4% severe anxiety symptoms. Conclusions: The prevalence of depressive and anxiety symptoms were substantially higher than those previously found for the Portuguese population and when compared with other countries during the pandemic. Younger individuals, female, with chronic illness and medicated, were more vulnerable to depressive and anxious symptoms. In contrast, participants who maintained frequent levels of physical activity during confinement had their mental health protected.
Variation in Communication and Family Visiting Policies in Italian Intensive Care Units during the COVID-19 Pandemic: A Secondary Analysis of the COVISIT International Survey
Authors: Simone Barbara , Ippolito Mariachiara , Iozzo Pasquale , Zuccaro Francesco , Giarratano Antonino , Cecconi Maurizio , Tabah Alexis , Cortegiani Andrea
Published: February 2023

Background: During COVID-19 pandemic, restrictions to in-person visiting of caregivers to patients admitted to intensive care units (ICU) were applied in many countries. Our aim was to describe the variations in communication and family visiting policies in Italian ICUs during the pandemic. Methods: A secondary analysis from the COVISIT international survey was conducted, focusing on data from Italy. Results: Italian ICUs provided 118 (18%) responses out of 667 responses collected worldwide. A total of 12 Italian ICUs were at the peak of COVID-19 admissions at the time of the survey and 42/118 had 90% or more of patients admitted to ICU affected by COVID-19. During the COVID-19 peak, 74% of Italian ICUs adopted a no-in-person-visiting policy. This remained the most common strategy (67%) at the time of the survey. Information to families was provided by regular phone calls (81% in Italy versus 47% for the rest of the world). Virtual visiting was available for 69% and most commonly performed using devices provided by the ICU (71% in Italy versus 36% outside Italy). Conclusion: Our study showed that restrictions to the ICU applied during the COVID-19 pandemic were still in use at the time of the survey. The main means of communication with caregivers were telephone and virtual meetings.
Development of the Menu Assessment Scoring Tool (MAST) to Assess the Nutritional Quality of Food Service Menus
Authors: Pulker Claire Elizabeth , Aberle Leisha Michelle , Butcher Lucy Meredith , Whitton Clare , Law Kristy Karying , Large Amy Louise , Pollard Christina Mary , Trapp Georgina S. A.
Published: February 2023

Preventing the rise in obesity is a global public health priority. Neighbourhood environments can help or undermine people’s efforts to manage their weight, depending on availability of nutritious and nutrient-poor ‘discretionary’ foods. The proportion of household food budgets spent on eating outside the home is increasing. To inform nutrition policy at a local level, an objective assessment of the nutritional quality of foods and beverages on food service menus that is context-specific is needed. This study describes the development and piloting of the Menu Assessment Scoring Tool (MAST), used to assess the nutritional quality of food service menus in Australia. The MAST is a desk-based tool designed to objectively assess availability of nutrient-poor and absence of nutritious food and beverages on food service menus. A risk assessment approach was applied, using the best available evidence in an iterative way. MAST scores for 30 food service outlets in one Local Government Authority in Perth, Western Australia highlight opportunities for improvements. MAST is the first tool of its kind in Australia to assess the nutritional quality of food service menus. It was practical and feasible to use by public health nutritionists/dietitians and can be adapted to suit other settings or countries.
Design and Realization of Rural Environment Art Construction of Cultural Image and Visual Communication
Authors: Liu Fulong , Lin Baogang , Meng Kun
Published: February 2023

The practice of rural construction has been exploring and trying to adapt to the needs of rural development in various periods. In recent years, under the attention and promotion of the central policy, various social forces have joined the ranks of rural construction, and art intervention in rural construction has begun as a new method. Entering the public eye, it deeply intervenes in the construction and development of the countryside in a more gentle way, from the key point of interaction between the social and cultural orientation and the material needs of the countryside. However, most of the art interventions in rural construction practice only unilaterally use artistic techniques to beautify local areas or display works, without realizing the hidden artistic and cultural value of the village and ignoring the participation and role of the villagers in the whole process. After the construction is completed, once the foreign construction forces are withdrawn, the development of the village will stagnate. Therefore, mobilizing the main body of rural construction (original villagers) to participate in the joint construction of the village is an important link to solve the current problems of art intervening in the construction of rural settlements.
Management for A Depressive Patient with Femoral Neck Fracture by Electroconvulsive Therapy during COVID-19 Pandemic: A Case Report and Literature Review
Authors: Chiu Nien-Mu , Tseng Ellen Yu-Lun
Published: February 2023

Electroconvulsive therapy (ECT) is an effective treatment for refractory major depressive disorder with suicidal ideation. The most common adverse medical events are transient retrograde amnesia, falls and pneumonia. Hip fractures, associated with high-energy trauma by convulsions, were occasionally reported in western countries, in the period before the COVID-19 pandemic. Strict COVID-19 regulations influenced the course and further investigation of the treatment of post-ECT complications. A 33-year-old man, previously diagnosed with major depressive disorder, had a history of nine successful sessions of ECT treatment for depression five years ago. He was hospitalized again for 12 sessions of ECT for recurrent depression. Unfortunately, an ECT-induced right hip–neck fracture was noted after the ninth session of ECT, in March 2021. After receiving close reduction and internal fixation of the right femoral neck fracture, with three screws, his original daily function was restored. His treatment was regularly followed up at the outpatient clinic for 20 months; he achieved partial remission with three combined antidepressants. This case of ECT-induced right hip–neck fracture informed psychiatric staff to be aware of this rare adverse complication and ensure its appropriate management, especially during the COVID-19 pandemic.
A Quantitative Study on Employees’ Experiences of a Support Model for Systematic Work Environment Management in Swedish Municipalities
Authors: Paulsson Sofia , Hellman Therese , Svartengren Magnus , Molin Fredrik
Published: February 2023

Today’s working life is constantly changing, and work environmental risk factors can alter swiftly. Besides the traditional physical work environment risk factors, somewhat more abstract organizational and social work environment factors also play an ever-increasing role, both in preventing and causing work-related illness. This requires a preventive work environment management that can respond to rapid changes, and where the assessment and remedies rely more on employee participation than on predetermined threshold limits. This study aimed to investigate if the use of a support model (the Stamina model) for workplace improvements could render the same positive effects in quantitative measures that have previously been shown in qualitative studies. Employees from six municipalities used the model for 12 months. They answered a questionnaire at baseline and after six and 12 months, to detect any changes in how they characterized their current work situation and perceived their influence, productivity, short-term recovery, and organizational justice. The results showed that employees felt more influential in work situations related to communication/collaboration and roles/tasks at the follow-up compared to the baseline. These results are consistent with previous qualitative studies. We found no significant changes in the other endpoints. The results strengthen previous conclusions, namely that the Stamina model can be used as part of inclusive, modern, and systematic work environment management.
Digital Inclusion, Cultural Capital, and Health Status of Urban and Rural Residents: An Empirical Study Based on 2017 CGSS Database
Authors: Sun Zhenyu , Sun Wei , Gao Hongliang , Fa Ruobing , Chen Shaofan , Qian Dongfu
Published: February 2023

China is committed to using digital technology to drive urban–rural integration in health care. This study aims to explore the effect of digital inclusion on health status with the mediating role of cultural capital and the digital health disparities between urban and rural residents in China. Using data from the 2017 Chinese General Social Survey (CGSS), the present study adopted an ordinary least squares (OLS) robust standard error regression model to investigate the impact of digital inclusion on health status. In addition, causal step regression (CSR) and bootstrapping methods were combined to test the mediating effect of cultural capital. The results showed that, first, digital inclusion was related to positive and significant effects on resident health status. Second, cultural capital played a mediating role in the relationship between digital inclusion and health status. Third, urban residents gained more health benefits from digital inclusion than rural residents. Additionally, common method variance (CMV) tests, endogenous tests, and a propensity score matching (PSM) analysis showed that the above conclusions remained robust. The government should therefore focus not only on promoting the population’s health by utilizing digital inclusion but also on accelerating digital health equity between urban and rural areas by developing such strategies as a digital infrastructure expansion schedule and digital literacy education and training programs.
Lifestyle Score and Risk of Hypertension in the Airwave Health Monitoring Study of British Police Force Employees
Authors: Aljuraiban Ghadeer S. , Gibson Rachel , Chan Doris S. M. , Elliott Paul , Chan Queenie , Griep Linda M. Oude
Published: February 2023

Background: Evidence suggest that promoting a combination of healthy lifestyle behaviors instead of exclusively focusing on a single behavior may have a greater impact on blood pressure (BP). We aimed to evaluate lifestyle factors and their impact on the risk of hypertension and BP. Methods: We analyzed cross-sectional health-screening data from the Airwave Health Monitoring Study of 40,462 British police force staff. A basic lifestyle-score including waist-circumference, smoking and serum total cholesterol was calculated, with a greater value indicating a better lifestyle. Individual/combined scores of other lifestyle factors (sleep duration, physical activity, alcohol intake, and diet quality) were also developed. Results: A 1-point higher basic lifestyle-score was associated with a lower systolic BP (SBP; −2.05 mmHg, 95%CI: −2.15, −1.95); diastolic BP (DBP; −1.98 mmHg, 95%CI: −2.05, −1.91) and was inversely associated with risk of hypertension. Combined scores of other factors showed attenuated but significant associations with the addition of sleep, physical activity, and diet quality to the basic lifestyle-score; however, alcohol intake did not further attenuate results. Conclusions: Modifiable intermediary factors have a stronger contribution to BP, namely, waist-circumference and cholesterol levels and factors that may directly influence them, such as diet, physical activity and sleep. Observed findings suggest that alcohol is a confounder in the BP–lifestyle score relation.
Multiple Intermediary Model Test of Adolescent Physical Exercise and Internet Addiction
Authors: Qiu Cheng , Qi Yufei , Yin Yao
Published: February 2023

On the basis of self-efficacy theory, self-control theory and psychological resilience theory, this paper discusses the relationship between physical exercise, self-efficacy, self-control, psychological resilience and Internet addiction among adolescents in Beijing. A convenience sampling method was used to conduct a questionnaire survey on physical activity and Internet addiction among 466 adolescents from first to third year in 10 high schools in Beijing, of which 41% were girls and 59% were boys; 1.9% of students were 14 years old, 42.5% were 15 years old, 23.4% were 16 years old, 31.3% were 17 years old and 0.9% were 18 years old. Using the research methods of the literature, correlation analysis and multiple intermediary structure model, this paper constructed and tested the multiple intermediary model between physical exercise and Internet addiction. The results show that physical exercise can significantly predict self-efficacy, psychological resilience and self-control; self-efficacy, psychological resilience and self-control significantly interfered with Internet addiction behaviour; there was a significant difference in the total effect of multiple intermediaries; the effect value was −0.173; the specific indirect effects of self-efficacy, psychological resilience and self-control had intermediary effects in the relationship between physical exercise and Internet addiction; and there was no difference in specific indirect effects. This paper puts forward some countermeasures and suggestions to prevent teenagers’ Internet addiction from cultivating, such as through good sports activities, thereby improving their Internet addiction. We should actively strive to improve teenagers’ deep understanding of the effect of physical exercise and gradually form physical exercise habits, with sports addiction replacing Internet addiction.
Selenium Species and Fractions in the Rock–Soil–Plant Interface of Maize (Zea mays L.) Grown in a Natural Ultra-Rich Se Environment
Authors: Pinzon-Nuñez Diego Armando , Wiche Oliver , Bao Zhengyu , Xie Shuyun , Fan Bolun , Zhang Wenkai , Tang Molan , Tian Huan
Published: February 2023

Selenium (Se) enrichments or deficiency in maize (Zea mays L.), one of the world’s most important staple foods and livestock feeds, can significantly affect many people’s diets, as Se is essential though harmful in excess. In particular, Se-rich maize seems to have been one of the factors that led to an outbreak of selenosis in the 1980s in Naore Valley in Ziyang County, China. Thus, this region’s geological and pedological enrichment offers some insight into the behavior of Se in naturally Se-rich crops. This study examined total Se and Se species in the grains, leaves, stalks, and roots of 11 maize plant samples, Se fractions of soils around the rhizosphere, and representative parent rock materials from Naore Valley. The results showed that total Se concentrations in the collected samples were observed in descending order of soil > leaf > root > grain > stalk. The predominant Se species detected in maize plants was SeMet. Inorganic Se forms, mainly Se(VI), decreased from root to grain, and were possibly assimilated into organic forms. Se(IV) was barely present. The natural increases of Se concentration in soils mainly affected leaf and root dry-weight biomasses of maize. In addition, Se distribution in soils markedly correlated with the weathered Se-rich bedrocks. The analyzed soils had lower Se bioavailability than rocks, with Se accumulated predominantly as recalcitrant residual Se. Thus, the maize plants grown in these natural Se-rich soils may uptake Se mainly from the oxidation and leaching of the remaining organic-sulfide-bound Se fractions. A viewpoint shift from natural Se-rich soils as menaces to possibilities for growing Se-rich agricultural products is also discussed in this study.
Adherence to Mediterranean Diet in Individuals on Renal Replacement Therapy
Authors: Falbo Elisabetta , Porchetti Gabriele , Conte Caterina , Tarsitano Maria
Published: February 2023

Patients on renal replacement therapy are typically subject to several dietary restrictions; however, this approach has been questioned in recent years, with some suggesting that the Mediterranean diet might be beneficial. Data on the adherence to this diet and factors that influence it are scarce. We conducted a web survey among individuals on renal replacement therapy (dialysis or kidney transplant, KT) using the MEDI-LITE questionnaire to assess adherence to the Mediterranean diet and dietary habits in this population. Adherence to the Mediterranean diet was generally low, and significantly lower among participants on dialysis versus KT recipients (19.4% vs. 44.7%, p < 0.001). Being on dialysis, adopting fluid restrictions, and having a basic level of education were predictors of low adherence to the Mediterranean diet. Consumption of foods typically included in the Mediterranean diet, including fruit, legumes, fish, and vegetables, was generally low, particularly among those on dialysis. There is a need for strategies to improve both the adherence to and the quality of the diet among individuals on renal replacement therapy. This should be a shared responsibility between registered dietitians, physicians, and the patient.
Oesophageal Atresia: Prevalence in the Valencian Region (Spain) and Associated Anomalies
Authors: Agurto-Ramírez Adriana , García-Villodre Laura , Ruiz-Palacio Ana , Arribas-Díaz Berta , Barrachina-Bonet Laia , Páramo-Rodríguez Lucía , Zurriaga Óscar , Cavero-Carbonell Clara
Published: February 2023

The objective was to determine the prevalence of oesophageal atresia (OA) and describe the characteristics of OA cases diagnosed before the first year of life, born between 2007 and 2019, and residents in the Valencian Region (VR), Spain. Live births (LB), stillbirths (SB), and termination of pregnancy for fetal anomaly (TOPFA) diagnosed with OA were selected from the Congenital Anomalies population-based Registry of VR (RPAC-CV). The prevalence of OA per 10,000 births with 95% confidence interval was calculated, and socio-demographic and clinical variables were analyzed. A total of 146 OA cases were identified. The overall prevalence was 2.4/10,000 births, and prevalence by type of pregnancy ending was 2.3 in LB and 0.03 in both SB and TOPFA. A mortality rate of 0.03/1000 LB was observed. A relationship was found between case mortality and birth weight (p-value < 0.05). OA was primarily diagnosed at birth (58.2%) and 71.2% of the cases were associated with another congenital anomaly, mainly congenital heart defects. Significant variations in the prevalence of OA in the VR were detected throughout the study period. In conclusion, a lower prevalence in SB and TOPFA was identified compared to EUROCAT data. As several studies have identified, an association between OA cases and birth weight was found.
Quality in Psychiatric Care in the Community Mental Health Setting from the Perspective of Patients and Staff
Authors: Roldán-Merino Juan Francisco , Tomás-Jiménez Manuel , Schröder Agneta , Lundqvist Lars-Olov , Puig-Llobet Montserrat , Moreno-Poyato Antonio R. , Domínguez del Campo Marta , Sanchez-Balcells Sara , Lluch-Canut Maria Teresa
Published: February 2023

The current paradigm of mental health care focuses on care provided in the community, increasingly moving away from hospital care models that involve considerable economic burden. Patient and staff perspectives on the quality of psychiatric care can highlight strengths and areas for improvement to ensure better care provision. The aim of this study was to describe and compare perceptions of quality of care among patients and staff in community mental health services and to determine possible relationships between these perceptions and other study variables. A comparative cross-sectional descriptive study was conducted in a sample of 200 patients and 260 staff from community psychiatric care services in the area of Barcelona (Spain). The results showed high overall levels of quality of care from patient (m = 104.35 ± 13.57) and staff (m =102.06 ± 8.80) perspectives. Patients and staff both gave high scores to Encounter and Support factors, while factors concerning patient Participation and Environment received the lowest scores. Continuous assessment of the quality of psychiatric care in the community setting is essential to ensure the highest quality of care, taking the perspectives of those involved into account.
Point Cloud Data Processing Optimization in Spectral and Spatial Dimensions Based on Multispectral Lidar for Urban Single-Wood Extraction
Authors: Shi Shuo , Tang Xingtao , Chen Bowen , Chen Biwu , Xu Qian , Bi Sifu , Gong Wei
Published: February 2023

Lidar can effectively obtain three-dimensional information on ground objects. In recent years, lidar has developed rapidly from single-wavelength to multispectral hyperspectral imaging. The multispectral airborne lidar Optech Titan is the first commercial system that can collect point cloud data on 1550, 1064, and 532 nm channels. This study proposes a method of point cloud segmentation in the preprocessed intensity interpolation process to solve the problem of inaccurate intensity at the boundary during point cloud interpolation. The entire experiment consists of three steps. First, a multispectral lidar point cloud is obtained using point cloud segmentation and intensity interpolation; the spatial dimension advantage of the multispectral point cloud is used to improve the accuracy of spectral information interpolation. Second, point clouds are divided into eight categories by constructing geometric information, spectral reflectance information, and spectral characteristics. Accuracy evaluation and contribution analysis are also conducted through point cloud truth value and classification results. Lastly, the spatial dimension information is enhanced by point cloud drop sampling, the method is used to solve the error caused by airborne scanning and single-tree extraction of urban trees. Classification results showed that point cloud segmentation before intensity interpolation can effectively improve the interpolation and classification accuracies. The total classification accuracy of the data is improved by 3.7%. Compared with the extraction result (377) of single wood without subsampling treatment, the result of the urban tree extraction proved the effectiveness of the proposed method with a subsampling algorithm in improving the accuracy. Accordingly, the problem of over-segmentation is solved, and the final single-wood extraction result (329) is markedly consistent with the real situation of the region.
Disorder of Golgi Apparatus Precedes Anoxia-Induced Pathology of Mitochondria
Authors: Morozov Yury M. , Rakic Pasko
Published: February 2023

Mitochondrial malfunction and morphologic disorganization have been observed in brain cells as part of complex pathological changes. However, it is unclear what may be the role of mitochondria in the initiation of pathologic processes or if mitochondrial disorders are consequences of earlier events. We analyzed the morphologic reorganization of organelles in an embryonic mouse brain during acute anoxia using an immunohistochemical identification of the disordered mitochondria, followed by electron microscopic three-dimensional (3D) reconstruction. We found swelling of the mitochondrial matrix after 3 h anoxia and probable dissociation of mitochondrial stomatin-like protein 2 (SLP2)-containing complexes after 4.5 h anoxia in the neocortex, hippocampus, and lateral ganglionic eminence. Surprisingly, deformation of the Golgi apparatus (GA) was detected already after 1 h of anoxia, when the mitochondria and other organelles still had a normal ultrastructure. The disordered GA showed concentrical swirling of the cisternae and formed spherical onion-like structures with the trans-cisterna in the center of the sphere. Such disturbance of the Golgi architecture likely interferes with its function for post-translational protein modification and secretory trafficking. Thus, the GA in embryonic mouse brain cells may be more vulnerable to anoxic conditions than the other organelles, including mitochondria.
Synthesis of 3-(2-Alkylthio-4-chloro-5-methylbenzenesulfonyl)-2-(1-phenyl-3-arylprop-2-enylideneamino)guanidine Derivatives with Pro-Apoptotic Activity against Cancer Cells
Authors: Pogorzelska Aneta , Sławiński Jarosław , Kawiak Anna , Stasiłojć Grzegorz , Chojnacki Jarosław
Published: February 2023

The untypical course of reaction between chalcones and benzenesulfonylaminoguanidines led to the new 3-(2-alkylthio-4-chloro-5-methylbenzenesulfonyl)-2-(1-phenyl-3-arylprop-2-enylideneamino)guanidine derivatives 8–33. The new compounds were tested in vitro for their impact on the growth of breast cancer cells MCF-7, cervical cancer cells HeLa and colon cancer cells HCT-116 by MTT assay. The results revealed that the activity of derivatives is strongly related to the presence of hydroxy group in the benzene ring at the 3-arylpropylidene fragment. The most cytotoxic compounds 20 and 24 displayed mean IC50 values of 12.8 and 12.7 μM, respectively, against three tested cell lines and were almost 3- and 4-fold more active toward MCF-7 and HCT-116 when compared with non-malignant HaCaT cells. Furthermore, compound 24 induced apoptosis in cancer cells and caused a decrease of mitochondrial membrane potential as well as an increase of cells in sub-G1 phase in contrast to its inactive analog 31. The strongest activity against the most sensitive HCT-116 cell line was found for compound 30 (IC50 = 8 μM), which was 11-fold more effective in the growth inhibition of HCT-116 cells than those of HaCaT cells. Based on this fact, the new derivatives may be promising leading structures for the search for agents for the treatment of colon cancer.
Biochemical Characteristics of iPSC-Derived Dopaminergic Neurons from N370S GBA Variant Carriers with and without Parkinson’s Disease
Authors: Grigor’eva Elena V. , Kopytova Alena E. , Yarkova Elena S. , Pavlova Sophia V. , Sorogina Diana A. , Malakhova Anastasia A. , Malankhanova Tuyana B. , Baydakova Galina V. , Zakharova Ekaterina Y. , Medvedev Sergey P. , Pchelina Sofia N. , Zakian Suren M.
Published: February 2023

GBA variants increase the risk of Parkinson’s disease (PD) by 10 times. The GBA gene encodes the lysosomal enzyme glucocerebrosidase (GCase). The p.N370S substitution causes a violation of the enzyme conformation, which affects its stability in the cell. We studied the biochemical characteristics of dopaminergic (DA) neurons generated from induced pluripotent stem cells (iPSCs) from a PD patient with the GBA p.N370S mutation (GBA-PD), an asymptomatic GBA p.N370S carrier (GBA-carrier), and two healthy donors (control). Using liquid chromatography with tandem mass spectrometry (LC-MS/MS), we measured the activity of six lysosomal enzymes (GCase, galactocerebrosidase (GALC), alpha-glucosidase (GAA), alpha-galactosidase (GLA), sphingomyelinase (ASM), and alpha-iduronidase (IDUA)) in iPSC-derived DA neurons from the GBA-PD and GBA-carrier. DA neurons from the GBA mutation carrier demonstrated decreased GCase activity compared to the control. The decrease was not associated with any changes in GBA expression levels in DA neurons. GCase activity was more markedly decreased in the DA neurons of GBA-PD patient compared to the GBA-carrier. The amount of GCase protein was decreased only in GBA-PD neurons. Additionally, alterations in the activity of the other lysosomal enzymes (GLA and IDUA) were found in GBA-PD neurons compared to GBA-carrier and control neurons. Further study of the molecular differences between the GBA-PD and the GBA-carrier is essential to investigate whether genetic factors or external conditions are the causes of the penetrance of the p.N370S GBA variant.
Novel Biomarkers for Diagnosis and Monitoring of Immune Thrombocytopenia
Authors: Allegra Alessandro , Cicero Nicola , Mirabile Giuseppe , Giorgianni Concetto Mario , Gangemi Sebastiano
Published: February 2023

Lower-than-normal platelet counts are a hallmark of the acquired autoimmune illness known as immune thrombocytopenia, which can affect both adults and children. Immune thrombocytopenia patients’ care has evolved significantly in recent years, but the disease’s diagnosis has not, and it is still only clinically achievable with the elimination of other causes of thrombocytopenia. The lack of a valid biomarker or gold-standard diagnostic test, despite ongoing efforts to find one, adds to the high rate of disease misdiagnosis. However, in recent years, several studies have helped to elucidate a number of features of the disease’s etiology, highlighting how the platelet loss is not only caused by an increase in peripheral platelet destruction but also involves a number of humoral and cellular immune system effectors. This made it possible to identify the role of immune-activating substances such cytokines and chemokines, complement, non-coding genetic material, the microbiome, and gene mutations. Furthermore, platelet and megakaryocyte immaturity indices have been emphasized as new disease markers, and prognostic signs and responses to particular types of therapy have been suggested. Our review’s goal was to compile information from the literature on novel immune thrombocytopenia biomarkers, markers that will help us improve the management of these patients.
Pyrrolidine Dithiocarbamate Suppresses Cutibacterium acnes-Induced Skin Inflammation
Authors: Shin Jin Hak , Kim Seon Sook , Seo Su Ryeon
Published: February 2023

Cutibacterium acnes (C. acnes), a Gram-positive anaerobic bacterium, proliferates in hair follicles and pores and causes inflammation in the skin of young people. The rapid growth of C. acnes triggers macrophages to secrete proinflammatory cytokines. Pyrrolidine dithiocarbamate (PDTC) is a thiol compound that exerts antioxidant and anti-inflammatory effects. Although the anti-inflammatory function of PDTC in several inflammatory disorders has been reported, the effect of PDTC on C. acnes-induced skin inflammation remains unexplored. In the present study, we examined the effect of PDTC on C. acnes-induced inflammatory responses and determined the mechanism by using in vitro and in vivo experimental models. We found that PDTC significantly inhibited the expression of C. acnes-induced proinflammatory mediators, such as interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and NOD-like receptor (NLR) pyrin domain-containing 3 (NLRP3), in mouse-bone-marrow-derived macrophage (BMDM) cells. PDTC suppressed C. acnes-induced activation of nuclear factor-kappa B (NF-κB), which is the major transcription factor for proinflammatory cytokine expression. In addition, we found that PDTC inhibited caspase-1 activation and IL-1β secretion through suppressing NLRP3 and activated the melanoma 2 (AIM2) inflammasome but not the NLR CARD-containing 4 (NLRC4) inflammasome. Moreover, we found that PDTC improved C. acnes-induced inflammation by attenuating C. acnes-induced IL-1β secretion in a mouse acne model. Therefore, our results suggest that PDTC has potential therapeutic value for the amelioration of C. acnes-induced skin inflammation.
PtrVCS2 Regulates Drought Resistance by Changing Vessel Morphology and Stomatal Closure in Populus trichocarpa
Authors: Li Meng , Dong Hao , Li Jiyuan , Dai Xiufang , Lin Jiaojiao , Li Shuang , Zhou Chenguang , Chiang Vincent L. , Li Wei
Published: February 2023

Drought has severe effects on plant growth, forest productivity, and survival throughout the world. Understanding the molecular regulation of drought resistance in forest trees can enable effective strategic engineering of novel drought-resistant genotypes of tree species. In this study, we identified a gene, PtrVCS2, encoding a zinc finger (ZF) protein of the ZF-homeodomain transcription factor in Populus trichocarpa (Black Cottonwood) Torr. & A. Gray. ex Hook. Overexpression of PtrVCS2 (OE-PtrVCS2) in P. trichocarpa resulted in reduced growth, a higher proportion of smaller stem vessels, and strong drought-resistance phenotypes. Stomatal movement experiments revealed that the OE-PtrVCS2 transgenics showed lower stomata apertures than wild-type plants under drought conditions. RNA-seq analysis of the OE-PtrVCS2 transgenics showed that PtrVCS2 regulates the expression of multiple genes involved in regulation of stomatal opening and closing, particularly the PtrSULTR3;1-1 gene, and several genes related to cell wall biosynthesis, such as PtrFLA11-12 and PtrPR3-3. Moreover, we found that the water use efficiency of the OE-PtrVCS2 transgenic plants was consistently higher than that of wild type plants when subjected to chronic drought stress. Taken together, our results suggest that PtrVCS2 plays a positive role in improving drought adaptability and resistance in P. trichocarpa.
Mitochondrial Alterations in Prostate Cancer: Roles in Pathobiology and Racial Disparities
Authors: Vikramdeo Kunwar Somesh , Sharma Amod , Anand Shashi , Sudan Sarabjeet Kour , Singh Seema , Singh Ajay Pratap , Dasgupta Santanu
Published: February 2023

Prostate cancer (PCa) affects millions of men worldwide and is a major cause of cancer-related mortality. Race-associated PCa health disparities are also common and are of both social and clinical concern. Most PCa is diagnosed early due to PSA-based screening, but it fails to discern between indolent and aggressive PCa. Androgen or androgen receptor-targeted therapies are standard care of treatment for locally advanced and metastatic disease, but therapy resistance is common. Mitochondria, the powerhouse of cells, are unique subcellular organelles that have their own genome. A large majority of mitochondrial proteins are, however, nuclear-encoded and imported after cytoplasmic translation. Mitochondrial alterations are common in cancer, including PCa, leading to their altered functions. Aberrant mitochondrial function affects nuclear gene expression in retrograde signaling and promotes tumor-supportive stromal remodeling. In this article, we discuss mitochondrial alterations that have been reported in PCa and review the literature related to their roles in PCa pathobiology, therapy resistance, and racial disparities. We also discuss the translational potential of mitochondrial alterations as prognostic biomarkers and as effective targets for PCa therapy.
Characterization of Iflavirus in the Red Flour Beetle, Tribolium castaneum (Coleoptera; Tenebrionidae)
Authors: Fatehi Soheila , Aikins Michael , Philips Thomas W. , Brown Susan , Zhu Kun Yan , Scully Erin D. , Park Yoonseong
Published: February 2023

Iflavirus is a group of viruses distributed mainly in arthropod species. We surveyed Tribolium castaneum iflavirus (TcIV) in different laboratory strains and in Sequence Read Archives (SRA) in GenBank. TcIV is highly specific to only T. castaneum and is not found in seven other Tenebrionid species, including the closely related species T. freemani. The same strains from different laboratories and different strains displayed largely different degrees of infections in the examination of 50 different lines by using Taqman-based quantitative PCR. We found that ~63% (27 out of 43 strains) of T. castaneum strains in different laboratories are positive for TcIV PCR with large degrees of variation, in the range of seven orders of magnitude, indicating that the TcIV is highly fluctuating depending on the rearing conditions. The TcIV was prevalent in the nervous system with low levels found in the gonad and gut. The transovarial transmission was supported in the experiment with surface-sterilized eggs. Interestingly, TcIV infection did not show observable pathogenicity. TcIV offers an opportunity to study the interaction between the virus and the immune system of this model beetle species.
Mosquito-Borne Diseases and Their Control Strategies: An Overview Focused on Green Synthesized Plant-Based Metallic Nanoparticles
Authors: Onen Hudson , Luzala Miryam M. , Kigozi Stephen , Sikumbili Rebecca M. , Muanga Claude-Josué K. , Zola Eunice N. , Wendji Sébastien N. , Buya Aristote B. , Balciunaitiene Aiste , Viškelis Jonas , Kaddumukasa Martha A. , Memvanga Patrick B.
Published: February 2023

Mosquitoes act as vectors of pathogens that cause most life-threatening diseases, such as malaria, Dengue, Chikungunya, Yellow fever, Zika, West Nile, Lymphatic filariasis, etc. To reduce the transmission of these mosquito-borne diseases in humans, several chemical, biological, mechanical, and pharmaceutical methods of control are used. However, these different strategies are facing important and timely challenges that include the rapid spread of highly invasive mosquitoes worldwide, the development of resistance in several mosquito species, and the recent outbreaks of novel arthropod-borne viruses (e.g., Dengue, Rift Valley fever, tick-borne encephalitis, West Nile, yellow fever, etc.). Therefore, the development of novel and effective methods of control is urgently needed to manage mosquito vectors. Adapting the principles of nanobiotechnology to mosquito vector control is one of the current approaches. As a single-step, eco-friendly, and biodegradable method that does not require the use of toxic chemicals, the green synthesis of nanoparticles using active toxic agents from plant extracts available since ancient times exhibits antagonistic responses and broad-spectrum target-specific activities against different species of vector mosquitoes. In this article, the current state of knowledge on the different mosquito control strategies in general, and on repellent and mosquitocidal plant-mediated synthesis of nanoparticles in particular, has been reviewed. By doing so, this review may open new doors for research on mosquito-borne diseases.