bluuebunny/crossref_metadata_2025 huggingface dataset:
WGS Analysis of Clonal and Plasmidic Epidemiology of Colistin-Resistance Mediated by mcr Genes in the Poultry Sector in Lebanon
Authors: Al-Mir Hiba , Osman Marwan , Drapeau Antoine , Hamze Monzer , Madec Jean-Yves , Haenni Marisa
Published: March 2021

Poultry and poultry meat are important contributors to the global antimicrobial burden. Unregulated and illegal use of extended-spectrum cephalosporins (ESC) in this sector has long been identified as a major cause of massive spread of ESC-resistant Escherichia coli, and colistin usage is considered a main driver of plasmid-mediated mcr genes dissemination. In Lebanon, the first mcr-1-positive E. coli found in poultry dates back to 2015, followed by a few reports of mcr-1-positive E. coli in poultry, swine, humans, and the environment. On the contrary, a comprehensive picture of the population structure of mcr-1-positive E. coli and mcr-1-bearing plasmids carrying the mcr-1 gene using whole-genome analysis is largely lacking. This study reports the prevalence of mcr-1-positive E. coli in poultry originating from 32 farms across three Lebanese governorates and slaughtered in the same place. We report 27/32 (84.4%) mcr-1 positive farms, leading to a total of 84 non-duplicate E. coli collected, of which 62 presented the mcr-1 gene. Numerous associated resistances were identified, including to ESC through the presence of blaCTX–M or blaCMY genes. The mcr-1 gene was mostly carried by IncX4 (n = 36) and IncI2 (n = 24) plasmids, which are both known for their efficient transfer capacities. A high genetic diversity was detected, arguing for the lack of contamination during the slaughter process. ST744 and ST1011 were the most widely identified clones, which have been both regularly associated to mcr-1-carrying E. coli and to the poultry sector. The wide dissemination of colistin-resistance, coupled to resistances to ESC and numerous other molecules, should urge authorities to implement efficient guidelines for the use of antibiotics in the poultry sector in Lebanon.
Membrane Remodeling by Arc/Arg3.1
Authors: Hedde Per Niklas , Malacrida Leonel , Barylko Barbara , Binns Derk D. , Albanesi Joseph P. , Jameson David M.
Published: March 2021

The activity-regulated cytoskeletal-associated protein (Arc, also known as Arg3.1) is an immediate early gene product induced by activity/experience and required for multiple modes of synaptic plasticity. Both long-term potentiation (LTP) and long-term depression (LTD) are impaired upon Arc deletion, as well as the ability to form long-term spatial, taste and fear memories. The best-characterized cellular function of Arc is enhancement of the endocytic internalization of AMPA receptors (AMPARs) in dendritic spines. Solution of the crystal structure of a C-terminal segment of Arc revealed a striking similarity to the capsid domain of HIV Gag. It was subsequently shown that Arc assembles into viral capsid-like structures that enclose Arc mRNA, are released into the extracellular space, and are internalized by neighboring cells. Thus, Arc is unique in participating in plasma membrane budding both into and out of the cell. In this report we study the interaction of Arc with membranes using giant unilamellar vesicles (GUVs). Using the fluorescent lipid probe LAURDAN, we find that Arc promotes the formation of smaller vesicles that penetrate into the GUV interior. Our results suggest that Arc induces negative membrane curvature and may therefore facilitate the formation of mRNA-containing extracellular vesicles from the plasma membrane.
Modeling and Simulation of a Human Knee Exoskeleton's Assistive Strategies and Interaction
Authors: Zhang Longbin , Liu Yixing , Wang Ruoli , Smith Christian , Gutierrez-Farewik Elena M.
Published: March 2021

Exoskeletons are increasingly used in rehabilitation and daily life in patients with motor disorders after neurological injuries. In this paper, a realistic human knee exoskeleton model based on a physical system was generated, a human–machine system was created in a musculoskeletal modeling software, and human–machine interactions based on different assistive strategies were simulated. The developed human–machine system makes it possible to compute torques, muscle impulse, contact forces, and interactive forces involved in simulated movements. Assistive strategies modeled as a rotational actuator, a simple pendulum model, and a damped pendulum model were applied to the knee exoskeleton during simulated normal and fast gait. We found that the rotational actuator–based assistive controller could reduce the user's required physiological knee extensor torque and muscle impulse by a small amount, which suggests that joint rotational direction should be considered when developing an assistive strategy. Compared to the simple pendulum model, the damped pendulum model based controller made little difference during swing, but further decreased the user's required knee flexor torque during late stance. The trade-off that we identified between interaction forces and physiological torque, of which muscle impulse is the main contributor, should be considered when designing controllers for a physical exoskeleton system. Detailed information at joint and muscle levels provided in this human–machine system can contribute to the controller design optimization of assistive exoskeletons for rehabilitation and movement assistance.
The Impact of Estrogen and Estrogen-Like Molecules in Neurogenesis and Neurodegeneration: Beneficial or Harmful?
Authors: Bustamante-Barrientos Felipe A. , Méndez-Ruette Maxs , Ortloff Alexander , Luz-Crawford Patricia , Rivera Francisco J. , Figueroa Carlos D. , Molina Luis , Bátiz Luis Federico
Published: March 2021

Estrogens and estrogen-like molecules can modify the biology of several cell types. Estrogen receptors alpha (ERα) and beta (ERβ) belong to the so-called classical family of estrogen receptors, while the G protein-coupled estrogen receptor 1 (GPER-1) represents a non-classical estrogen receptor mainly located in the plasma membrane. As estrogen receptors are ubiquitously distributed, they can modulate cell proliferation, differentiation, and survival in several tissues and organs, including the central nervous system (CNS). Estrogens can exert neuroprotective roles by acting as anti-oxidants, promoting DNA repair, inducing the expression of growth factors, and modulating cerebral blood flow. Additionally, estrogen-dependent signaling pathways are involved in regulating the balance between proliferation and differentiation of neural stem/progenitor cells (NSPCs), thus influencing neurogenic processes. Since several estrogen-based therapies are used nowadays and estrogen-like molecules, including phytoestrogens and xenoestrogens, are omnipresent in our environment, estrogen-dependent changes in cell biology and tissue homeostasis have gained attention in human health and disease. This article provides a comprehensive literature review on the current knowledge of estrogen and estrogen-like molecules and their impact on cell survival and neurodegeneration, as well as their role in NSPCs proliferation/differentiation balance and neurogenesis.
Changes in Food Consumption During the COVID-19 Pandemic: Analysis of Consumer Survey Data From the First Lockdown Period in Denmark, Germany, and Slovenia
Authors: Janssen Meike , Chang Betty P. I. , Hristov Hristo , Pravst Igor , Profeta Adriano , Millard Jeremy
Published: March 2021

This paper focuses on changes in food consumption that occurred during the COVID-19 pandemic. Its objective is to map changes at individual consumer level and identify the influence of different factors related to the COVID-19 pandemic on changes in individual food consumption. We conducted a cross-sectional online survey among 2,680 residents of Denmark (DK), Germany (DE), and Slovenia (SI) using quota sampling for gender, age and regional distribution. Data on consumption frequencies before and during the pandemic were collected with a food frequency questionnaire in the spring of 2020 (during the first lockdown period) for important types of fresh food and non-perishable food. Our results showed that, depending on the type of food, 15–42% of study participants changed their consumption frequency during the pandemic, compared to before. In all the study countries, the food categories with the highest rates of change were frozen food, canned food, and cake and biscuits; among the food categories with lower rates of change were bread, alcoholic drinks, and dairy products. People across all three countries shopped less frequently during lockdown and there was an overall reduction in the consumption of fresh foods, but an increase in the consumption of food with a longer shelf life in Denmark and Germany. Interestingly though, we observed diverging trends in all food categories analyzed, with some people decreasing and others increasing their consumption frequencies, demonstrating that the pandemic had different impacts on people's lifestyles and food consumption patterns. Using the method of multinomial regression analysis, we identified factors significantly (p< 0.01,p< 0.05,p< 0.1) related to increases and decrease in individuals' consumption frequencies in different food categories. The factors include restrictions put in place in response to the pandemic (i.e., closure of physical workplaces, canteens, cafés and restaurants, schools, and childcare institutions), changes in households' grocery shopping frequency, individuals' perceived risk of COVID-19, income losses due to the pandemic, and socio-demographic factors. Interesting differences between the countries were detected, allowing insights into the different food cultures. Conclusions include implications for policy-makers and actors in the food supply chain on the issues of healthy diets, food system resilience, and behavior change.
Remodeling of Stromal Cells and Immune Landscape in Microenvironment During Tumor Progression
Authors: Arora Leena , Pal Durba
Published: March 2021

The molecular understanding of carcinogenesis and tumor progression rests in intra and inter-tumoral heterogeneity. Solid tumors confined with vast diversity of genetic abnormalities, epigenetic modifications, and environmental cues that differ at each stage from tumor initiation, progression, and metastasis. Complexity within tumors studied by conventional molecular techniques fails to identify different subclasses in stromal and immune cells in individuals and that affects immunotherapies. Here we focus on diversity of stromal cell population and immune inhabitants, whose subtypes create the complexity of tumor microenvironment (TME), leading primary tumors towards advanced-stage cancers. Recent advances in single-cell sequencing (epitope profiling) approach circumscribes phenotypic markers, molecular pathways, and evolutionary trajectories of an individual cell. We discussed the current knowledge of stromal and immune cell subclasses at different stages of cancer development with the regulatory role of non-coding RNAs. Finally, we reported the current therapeutic options in immunotherapies, advances in therapies targeting heterogeneity, and possible outcomes.
Neoadjuvant Chemoradiotherapy Does Not Contribute to Worse Survival in Pathological Node-Negative Rectal Cancer
Authors: Huang Yong , Wei Wei , Wang Zhenguang , Liang Tao , Tian Shuyun , Fu Guangshun
Published: March 2021

Purpose: The prognostic significance of ypN0 rectal cancer with comparison to pN0 disease still remains poorly defined. This study aimed to compare the prognosis of ypN0 and pN0 rectal cancer.

Methods: Eligible patients were identified from the SEER18 registries research database (the latest data up to date was on April 15, 2019). Propensity score (PS) matching was usually performed to reduce the imbalance and potential confounding that were introduced by inherent differences between the groups. The cause-specific survival (CSS) was analyzed to evaluate the prognostic prediction of ypN0 and pN0 groups using the Kaplan–Meier method with the log-rank test. Cox proportional hazard model was also used to identify independent prognostic variables.

Results: In total, 26,832 patients diagnosed with pN0 or ypN0 rectal cancer were confirmed as the final cohort, including 7,237 (27.0%) patients with radiation and 19,595 (73.0%) patients without radiation prior to surgery. The median follow-up time was up to 81 months. After adjusting for other prognostic factors, neoadjuvant radiotherapy was not an independent prognostic variable of CSS (HR = 1.100, 95%CI = 0.957–1.265, P = 0.180, using pN0 group as the reference).

Conclusions: ypN0 rectal cancer was strongly associated with worse pathological diagnoses compared with pN0 rectal cancer, contributing to worse oncologic outcomes. However, the receipt of neoadjuvant chemoradiotherapy was not an independent prognostic factor of worse prognosis in pathological node-negative patients. Our study could give guidance to the treatment of ypN0 rectal cancer.
Cinical, Metabolic, and Genetic Analysis and Follow-Up of Eight Patients With HIBCH Mutations Presenting With Leigh/Leigh-Like Syndrome
Authors: Wang Junling , Liu Zhimei , Xu Manting , Han Xiaodi , Ren Changhong , Yang Xinying , Zhang Chunhua , Fang Fang
Published: March 2021

3-Hydroxyisobutyryl-CoA hydrolase (HIBCH, NM_014362.3) gene mutation can cause HIBCH deficiency, leading to Leigh/Leigh-like disease. To date, few case series have investigated the relationship between metabolites and clinical phenotypes or the effects of treatment, although 34 patients with HIBCH mutations from 27 families have been reported. The purpose of this study was to analyze the phenotypic spectrum, follow-up results, metabolites, and genotypes of patients with HIBCH deficiency presenting with Leigh/Leigh-like syndrome and explore specific metabolites related to disease diagnosis and prognosis through retrospective and longitudinal studies. Applying next-generation sequencing, we identified eight patients with HIBCH mutations from our cohort of 181 cases of genetically diagnosed Leigh/Leigh-like syndrome. Six novel HIBCH mutations were identified: c.977T>G [p.Leu326Arg], c.1036G>T [p.Val346Phe], c.750+1G>A, c.810-2A>C, c.469C>T [p.Arg157*], and c.236delC [p.Pro79Leufs*5]. The Newcastle Pediatric Mitochondrial Disease Scale (NPMDS) was employed to assess disease progression and clinical outcomes. The non-invasive approach of metabolite analysis showed that levels of some were associated with clinical phenotype severity. Five (5/7) patients presented with elevated C4-OH in dried blood spots, and the level was probably correlated with the NPMDS scores during the peak disease phase. 2,3-Dihydroxy-2-methylbutyrate in urine was elevated in six (6/7) patients and elevated S-(2-caboxypropyl)cysteamine in urine was found in three patients (3/3). The median age at initial presentation was 13 months (8–18 months), and the median follow-up was 2.3 years (range 1.3–7.2 years). We summarized and compared with all reported patients with HIBCH mutations. The most prominent clinical manifestations were developmental regression/delay, hypotonia, encephalopathy, and feeding difficulties. We administered drug and dietary treatment. During follow-up, five patients responded positively to treatment with a significant decrease in NPMDS scores. Our research is the largest case series of patients with HIBCH mutations.
A Bioassay-Based Approach for the Batch-To-Batch Consistency Evaluation of Xuesaitong Injection on a Zebrafish Thrombosis Model
Authors: Ma Xiangwei , Chen Yanyu , Jiang Shumin , Zhao Xiaoping
Published: March 2021

Quality control of Chinese medicine (CM) is mainly based on chemical testing, which sometimes shows weak correlation to pharmacological effects. Thus, there is a great demand to establish bioactivity-based assays to ensure the quality of CM. The aim of the present study was to establish a bioassay-based approach to evaluate the biological activity of Xuesaitong injection (XST) based on an in vivo zebrafish model. Zebrafish larvae with arachidonic acid (AA)-induced thrombus were applied to evaluate anti-thrombosis effects of XST and explore the potential mechanism of XST. Analysis of major components in normal and abnormal XST samples was performed by high performance liquid chromatography (HPLC). The results indicate that XST could significantly restore heart red blood cells (RBCs) intensity of thrombotic zebrafish in a dose-dependent manner, whilst decreasing RBCs accumulation in the caudal vein. The results were confirmed using a green fluorescence protein (GFP)-labeled zebrafish thrombosis model. Moreover, we could show that XST downregulates the expression of the fibrinogen alpha chain (fga) gene to inhibit the coagulation cascade during the process of thrombosis in zebrafish. Notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Rd, which were considered to be the major components of XST, also showed moderate anti-thrombosis efficacy. Further results showed that the zebrafish thrombosis model could efficiently distinguish five abnormal batches of XST from 24 normal batches. Furthermore, the inhibition rates of different batches were correlated with the content level of major components. Our results suggested that the proposed zebrafish thrombosis model could be successfully used to evaluate the batch-to-batch consistency of XST, which provided an alternative way for the quality control of CM.
Inhibition of TLR4/MAPKs Pathway Contributes to the Protection of Salvianolic Acid A Against Lipotoxicity-Induced Myocardial Damage in Cardiomyocytes and Obese Mice
Authors: Yang Zhen , Chen Yanli , Yan Zhaoyuan , Xu Tian Tian , Wu Xiangyao , Pi Aiwen , Liu Qingsheng , Chai Hui , Li Songtao , Dou Xiaobing
Published: March 2021

The occurrence of lipotoxicity during obesity-associated cardiomyopathy is detrimental to health. Salvianolic acid A (SAA), a natural polyphenol extract of Salvia miltiorrhiza Bunge (Danshen in China), is known to be cardioprotective. However, its clinical benefits against obesity-associated cardiomyocyte injuries are unclear. This study aimed at evaluating the protective effects of SAA against lipotoxicity-induced myocardial injury and its underlying mechanisms in high fat diet (HFD)-fed mice and in palmitate-treated cardiomyocyte cells (H9c2). Our analysis of aspartate aminotransferase and creatine kinase isoenzyme-MB (CM-KB) levels revealed that SAA significantly reversed HFD-induced myocardium morphological changes and improved myocardial damage. Salvianolic acid A pretreatment ameliorated palmitic acid-induced myocardial cell death and was accompanied by mitochondrial membrane potential and intracellular reactive oxygen species improvement. Analysis of the underlying mechanisms showed that SAA reversed myocardial TLR4 induction in HFD-fed mice and H9c2 cells. Palmitic acid-induced cell death was significantly reversed by CLI-95, a specific TLR4 inhibitor. TLR4 activation by LPS significantly suppressed SAA-mediated lipotoxicity protection. Additionally, SAA inhibited lipotoxicity-mediated expression of TLR4 target genes, including MyD88 and p-JNK/MAPK in HFD-fed mice and H9c2 cells. However, SAA did not exert any effect on palmitic acid-induced SIRT1 suppression and p-AMPK induction. In conclusion, our data shows that SAA protects against lipotoxicity-induced myocardial damage through a TLR4/MAPKs mediated mechanism.
Exploring the Interplay Between Adversity, Neurocognition, Social Cognition, and Functional Outcome in People With Psychosis: A Narrative Review
Authors: Rodriguez Victoria , Aas Monica , Vorontsova Natasha , Trotta Giulia , Gadelrab Romayne , Rooprai Navneet Kaur , Alameda Luis
Published: March 2021

History of adversity is associated with subsequent psychosis, and with a spectrum of cognitive alterations in individuals with psychosis. These cognitive features go from neurocognitive aspects as working memory and attention, to complex social cognitive processes as theory of mind and emotional perception. Difficulties in these domains impact patients' social and occupational functioning, which has been shown to be more impaired in those previously exposed to childhood trauma. However, the interplay between adversity, neurocognition, and functioning is yet poorly understood. This narrative review aims to explore the evidence on whether deficits in neurocognitive and social cognitive domains may act as possible putative mechanism linking adversity with functioning in people with psychosis. We show available evidence supporting the link between adversity and poorer functioning in psychosis, especially in chronic stages; and replicated evidence suggesting associations of social cognition and, to a lesser extent, neurocognition with impairment in functioning in patients; although there is still an important gap in the literature testing particularly deficits in social cognition as mediator of the link between adversity and functional decline in psychosis. Targeting interventions focusing on neurocognition and social cognition in individuals with adversity and psychosis seems important, given the severe deterioration of these patients in these domains, although more research is needed to test whether such treatments can specifically improve functioning in individuals with psychosis and adversity. Literature aiming to understand the determinants of functional outcome should consider the pervasive impact of childhood adversity, and its related effects on cognition.
Epistemic Corruption, the Pharmaceutical Industry, and the Body of Medical Science
Authors: Sismondo Sergio
Published: March 2021

When a knowledge system importantly loses integrity, ceasing to provide the kinds of trusted knowledge expected of it, we can label thisepistemic corruption. Epistemic corruption often occurs because the system has been co-opted for interests at odds with some of the central goals thought to lie behind it. There is now abundant evidence that the involvement of pharmaceutical companies corrupts medical science. Within the medical community, this is generally assumed to be the result of conflicts of interest. However, some important ways that the industry corrupts are not captured well by standard analyses in terms of conflicts of interest. It is not just that there is a body of medical science perverted by industry largesse. Instead, much of the corruption of medical science via the pharmaceutical industry happens through grafting activities: Pharmaceutical companies do their own research and smoothly integrate it with medical science, taking advantage of the legitimacy of the latter.
Why Do Animals Live in Cities?
Authors: Magle Seth B. , Kay Cria A. M. , Buckley Jacqueline , Fake Kimberly R. , Fidino Mason , Lehrer Elizabeth W. , Murray Maureen H.
Published: March 2021

Cities are intended to be places for people to live, but some animals survive and even thrive in cities. Animals that are smaller, have more general diets, and are more intelligent or adaptable are especially good at city life. Many of these wildlife species have learned special behaviors to help them survive in urban areas. Scientists use many tools to study these animals because understanding wildlife in cities can help people learn to live with them. There are many things you can do to help scientists learn more about the animals you see every day and make your neighborhood a better place for wild animals.
Differential Signals From TNFα-Treated and Untreated Embryos in Uterine Tissues and Splenic CD4+ T Lymphocytes During Preimplantation Pregnancy in Mice
Authors: Buska-Mach Katarzyna , Kedzierska Anna Ewa , Lepczynski Adam , Herosimczyk Agnieszka , Ozgo Małgorzata , Karpinski Pawel , Gomulkiewicz Agnieszka , Lorek Daria , Slawek Anna , Dziegiel Piotr , Chelmonska-Soyta Anna
Published: March 2021

The main aim of this study was to examine if a female mouse body in preimplantation pregnancy can distinguish between embryos of normal and impaired biological quality in the local and peripheral compartments. Normal (control group) and TNFα (tumor necrosis factor-α)-treated embryos (experimental group) at the morula stage were non-surgically transferred into the uteri of CD-1 strain [Crl:CD1(Icr)] female murine recipients. Twenty-four hours after the embryo transfer, females were euthanised, and uteri and spleens were dissected. In uterine tissues (local compartment), we assessed the expression of 84 genes comprising nine signal transduction pathways, using a modified RT2Profiler PCR Array. In the spleen (peripheral compartment), we determined the proteome of splenic CD4+lymphocytes using 2D protein electrophoresis with subsequent protein identification by mass spectrometry. Sample clustering and differential gene expression analyses within individual signal transduction pathways revealed differential expression of genes in the uteri of females after transplantation of normal vs. TNFα-treated embryos. The most affected signal transduction cascade was the NFKB (Nuclear factor NF-kappa-B) pathway, where 87.5% of the examined genes were significantly differentially expressed. Proteomic analysis of splenic CD4+T lymphocytes revealed significant differential expression of 8 out of 132 protein spots. Identified proteins were classified as proteins influenced by cell stress, proteins engaged in the regulation of cytoskeleton stabilization and cell motility, and proteins having immunomodulatory function. These results support the hypothesis that even before embryo implantation, the body of pregnant female mice can sense the biological quality of an embryo both at the local and peripheral level.
Increase in Tuberculosis Diagnostic Delay during First Wave of the COVID-19 Pandemic: Data from an Italian Infectious Disease Referral Hospital
Authors: Di Gennaro Francesco , Gualano Gina , Timelli Laura , Vittozzi Pietro , Di Bari Virginia , Libertone Raffaella , Cerva Carlotta , Pinnarelli Luigi , Nisii Carla , Ianniello Stefania , Mosti Silvia , Bevilacqua Nazario , Iacomi Fabio , Mondi Annalisa , Topino Simone , Goletti Delia , Vaia Francesco , Ippolito Giuseppe , Girardi Enrico , Palmieri Fabrizio
Published: March 2021

Background: The WHO advised that the impact of COVID-19 pandemic on TB services was estimated to be dramatic due to the disruption of TB services. Methods: A retrospective data collection and evaluation was conducted to include all the patients hospitalized for TB at INMI from 9 March to 31 August 2020 (lockdown period and three months thereafter). For the purpose of the study, data from patients hospitalized in the same period of 2019 were also collected. Results: In the period of March–August 2019, 201 patients were hospitalized with a diagnosis of TB, while in the same period of 2020, only 115 patients, with a case reduction of 43%. Patients with weight loss, acute respiratory failure, concurrent extrapulmonary TB, and higher Timika radiographic scores were significantly more frequently hospitalized during 2020 vs. 2019. The median patient delay was 75 days (IQR: 40–100) in 2020 compared to 30 days (IQR: 10–60) in 2019 (p < 0.01). Diagnostic delays in 2020 remain significant in the multiple logistic model (AOR = 6.93, 95%CI: 3.9–12.3). Conclusions: Our experience suggests that COVID-19 pandemic had an impact on TB patient care in terms of higher diagnostic delay, reduction in hospitalization, and a greater severity of clinical presentations.
Oxidized Albumin as a Mediator of Kidney Disease
Authors: Figueroa Stefanny M. , Araos Patricio , Reyes Javier , Gravez Basile , Barrera-Chimal Jonatan , Amador Cristián A.
Published: March 2021

Renal diseases are a global health concern, and nearly 24% of kidney disease patients are overweight or obese. Particularly, increased body mass index has been correlated with oxidative stress and urinary albumin excretion in kidney disease patients, also contributing to increased cardiovascular risk. Albumin is the main plasma protein and is able to partially cross the glomerular filtration barrier, being reabsorbed mainly by the proximal tubule through different mechanisms. However, it has been demonstrated that albumin suffers different posttranslational modifications, including oxidation, which appears to be tightly linked to kidney damage progression and is increased in obese patients. Plasma-oxidized albumin levels correlate with a decrease in estimated glomerular filtration rate and an increase in blood urea nitrogen in patients with chronic kidney disease. Moreover, oxidized albumin in kidney disease patients is independently correlated with higher plasma levels of transforming growth factor beta (TGF-β1), tumor necrosis factor (TNF-α), and interleukin (IL)-1β and IL-6. In addition, oxidized albumin exerts a direct effect on neutrophils by augmenting the levels of neutrophil gelatinase-associated lipocalin, a well-accepted biomarker for renal damage in patients and in different experimental settings. Moreover, it has been suggested that albumin oxidation occurs at early stages of chronic kidney disease, accelerating the patient requirements for dialytic treatment during disease progression. In this review, we summarize the evidence supporting the role of overweight- and obesity-induced oxidative stress as a critical factor for the progression of renal disease and cardiovascular morbimortality through albumin oxidation.
Utilization of Astaxanthin as a Synthetic Antioxidant Replacement for Emulsified Sausages
Authors: Seo Jin-Kyu , Parvin Rashida , Park Junyoung , Yang Han-Sul
Published: March 2021

The aim of this study was to evaluate the effect of astaxanthin (AX) on the quality and sensory characteristics of emulsified pork sausages during cold storage. The changes of Peroxide value (PV), 2-thiobarbituric acid-reactive substances (TBARS), thiol content, texture profile analysis, instrumental color, and sensorial qualities were assessed on specific storage days. The emulsified sausages with added AX exhibited significantly (p < 0.05) higher redness values and total color differences (ΔE) on all storage days. Sensory values recorded the reddest color and greater overall acceptability scores to the sample with AX. In addition, AX had a significantly (p < 0.05) greater effect on PV, TBARS, and thiol content of sausages, compared with the control (CON). AX showed higher oxidation stability than CON for regression coefficient, and the level of inhibition of malondialdehyde formation was similar to that of butylated hydroxytoluene (BHT) on storage days. Synthetically, AX had a desirable consequence on antioxidant activity and color of emulsified sausages; therefore, it can be used as a multifunctional additive in emulsified pork sausages.
Noncoding RNAs Associated with Therapeutic Resistance in Pancreatic Cancer
Authors: Son Seung Wan , Song Mun Gyu , Yun Ba Da , Park Jong Kook
Published: March 2021

Therapeutic resistance is an inevitable impediment towards effective cancer therapies. Evidence accumulated has shown that the signaling pathways and related factors are fundamentally responsible for therapeutic resistance via regulating diverse cellular events, such as epithelial-to-mesenchymal transition (EMT), stemness, cell survival/apoptosis, autophagy, etcetera. Noncoding RNAs (ncRNAs) have been identified as essential cellular components in gene regulation. The expression of ncRNAs is altered in cancer, and dysregulated ncRNAs participate in gene regulatory networks in pathological contexts. An in-depth understanding of molecular mechanisms underlying the modulation of therapeutic resistance is required to refine therapeutic benefits. This review presents an overview of the recent evidence concerning the role of human ncRNAs in therapeutic resistance, together with the feasibility of ncRNAs as therapeutic targets in pancreatic cancer.
Influence of Nitrogen-Doped Carbon Dot and Silver Nanoparticle Modified Carbon Paste Electrodes on the Potentiometric Determination of Tobramycin Sulfate: A Comparative Study
Authors: Fares Nermine V. , Medhat Passant M. , El Maraghy Christine M. , Okeil Sherif , Ayad Miriam F.
Published: March 2021

Two inexpensive and simple methods for synthesis of carbon nanodots were applied and compared to each other, namely a hydrothermal and microwave-assisted method. The synthesized carbon nanodots were characterized using transmission electron microscopy (TEM), ultraviolet-visible (UV-Vis), photoluminescence (PL), Fourier transform-infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The synthesized microwave carbon nanodots had smaller particle size and were thus chosen for better electrochemical performance. Therefore, they were used for our modification process. The proposed electrodes performance characteristics were evaluated according to the IUPAC guidelines, showing linear response in the concentration range 10−6–10−2, 10−7–10−2, and 10−8–10−2 M of tobramycin with a Nernstian slope of 52.60, 58.34, and 57.32 mV/decade for the bare, silver nanoparticle and carbon nanodots modified carbon paste electrodes, respectively. This developed potentiometric method was used for quantification of tobramycin in its co-formulated dosage form and spiked human plasma with good recovery percentages and without interference of the co-formulated drug loteprednol etabonate and excipients.
Preliminary Studies of Perovskite-Loaded Plastic Scintillator Prototypes for Radioactive Strontium Detection
Authors: Kang Hara , Min Sujung , Seo Bumkyung , Roh Changhyun , Hong Sangbum , Cheong Jae Hak
Published: March 2021

Functional plastic scintillators have attracted much attention for their usefulness in on-site monitoring and detection in environments. In this study, we elucidated a highly reliable and functional plastic scintillator for detection of radioactive strontium, which means a potent perovskite-loaded polymeric scintillation material based on epoxy and 2,5-diphenyloxazole (PPO). Moreover, Monte Carlo N-Particle (MCNP) simulation was performed to optimize the thickness of a plastic scintillator for efficient strontium detection. A thickness of 2 mm was found to be the optimum thickness for strontium beta-ray detection. A newly developed plastic scintillator with 430 nm emission from perovskite loading could trigger scintillation enhancement employing potential indication of perovskite energy transfer into a photomultiplier (PMT) detector. Furthermore, the response to beta-ray emitter of 90Sr was compared to commercial scintillator of BC-400 by exhibiting detection efficiency in the energy spectrum with a fabricated perovskite-loaded plastic scintillator. We believe that this suggested functional plastic scintillator could be employed as a radiation detector for strontium detection in a wide range of applications including decommissioning sites in nuclear facilities, nuclear security and monitoring, nonproliferation, and safeguards.
The Potential Role of Exosomes in Child and Adolescent Obesity
Authors: Maligianni Ioanna , Yapijakis Christos , Bacopoulou Flora , Chrousos George
Published: March 2021

Child and adolescent obesity constitute one of the greatest contemporary public health menaces. The enduring disproportion between calorie intake and energy consumption, determined by a complex interaction of genetic, epigenetic, and environmental factors, finally leads to the development of overweight and obesity. Child and adolescent overweight/obesity promotes smoldering systemic inflammation (“para-inflammation”) and increases the likelihood of later metabolic and cardiovascular complications, including metabolic syndrome and its components, which progressively deteriorate during adulthood. Exosomes are endosome-derived extracellular vesicles that are secreted by a variety of cells, are naturally taken-up by target cells, and may be involved in many physiological and pathological processes. Over the last decade, intensive research has been conducted regarding the special role of exosomes and the non-coding (nc) RNAs they contain (primarily micro (mi) RNAs, long (l) non-coding RNAs, messenger (m) RNAs and other molecules) in inter-cellular communications. Through their action as communication mediators, exosomes may contribute to the pathogenesis of obesity and associated disorders. There is increasing evidence that exosomal miRNAs and lncRNAs are involved in pivotal processes of adipocyte biology and that, possibly, play important roles in gene regulation linked to human obesity. This review aims to improve our understanding of the roles of exosomes and their cargo in the development of obesity and related metabolic and inflammatory disorders. We examined their potential roles in adipose tissue physiology and reviewed the scarce data regarding the altered patterns of circulating miRNAs and lncRNAs observed in obese children and adolescents, compared them to the equivalent, more abundant existing findings of adult studies, and speculated on their proposed mechanisms of action. Exosomal miRNAs and lncRNAs could be applied as cardiometabolic risk biomarkers, useful in the early diagnosis and prevention of obesity. Furthermore, the targeting of crucial circulating exosomal cargo to tissues involved in the pathogenesis and maintenance of obesity could provide a novel therapeutic approach to this devastating and management-resistant pandemic.
Petrified Ears: A Clue for Adrenal Insufficiency
Authors: Recalcati Sebastiano , Fantini Fabrizio
Published: March 2021

Petrified ears is an uncommon clinical entity. It describes auricular cartilage hardening, due usually to ectopic calcification or, less commonly, ossification. The most common causes are frostbite and mechanical trauma. However, endocrinopathies have also been reported to cause ectopic calcium deposition through an unknown mechanism. Addison’s disease is the systemic disease most frequently associated, but the exact pathogenesis remains unclear. Patients are usually asymptomatic, and the diagnosis is made incidentally when rigid helices are noted on palpation and can be confirmed by radiological imaging. A biopsy can also help to differentiate between calcification and ossification. The presence of this condition may be a useful clinical sign, which in some cases precedes the development of endocrinopathies by many years. We report on a case, and we review the current literature.
PAC-Bayes Bounds on Variational Tempered Posteriors for Markov Models
Authors: Banerjee Imon , Rao Vinayak A. , Honnappa Harsha
Published: March 2021

Datasets displaying temporal dependencies abound in science and engineering applications, with Markov models representing a simplified and popular view of the temporal dependence structure. In this paper, we consider Bayesian settings that place prior distributions over the parameters of the transition kernel of a Markov model, and seek to characterize the resulting, typically intractable, posterior distributions. We present a Probably Approximately Correct (PAC)-Bayesian analysis of variational Bayes (VB) approximations to tempered Bayesian posterior distributions, bounding the model risk of the VB approximations. Tempered posteriors are known to be robust to model misspecification, and their variational approximations do not suffer the usual problems of over confident approximations. Our results tie the risk bounds to the mixing and ergodic properties of the Markov data generating model. We illustrate the PAC-Bayes bounds through a number of example Markov models, and also consider the situation where the Markov model is misspecified.
The 2-D Cluster Variation Method: Topography Illustrations and Their Enthalpy Parameter Correlations
Authors: Maren Alianna J.
Published: March 2021

One of the biggest challenges in characterizing 2-D image topographies is finding a low-dimensional parameter set that can succinctly describe, not so much image patterns themselves, but the nature of these patterns. The 2-D cluster variation method (CVM), introduced by Kikuchi in 1951, can characterize very local image pattern distributions using configuration variables, identifying nearest-neighbor, next-nearest-neighbor, and triplet configurations. Using the 2-D CVM, we can characterize 2-D topographies using just two parameters; the activation enthalpy (ε0) and the interaction enthalpy (ε1). Two different initial topographies (“scale-free-like” and “extreme rich club-like”) were each computationally brought to a CVM free energy minimum, for the case where the activation enthalpy was zero and different values were used for the interaction enthalpy. The results are: (1) the computational configuration variable results differ significantly from the analytically-predicted values well before ε1 approaches the known divergence as ε1→0.881, (2) the range of potentially useful parameter values, favoring clustering of like-with-like units, is limited to the region where ε0<3 and ε1<0.25, and (3) the topographies in the systems that are brought to a free energy minimum show interesting visual features, such as extended “spider legs” connecting previously unconnected “islands,” and as well as evolution of “peninsulas” in what were previously solid masses.
CEPS: An Open Access MATLAB Graphical User Interface (GUI) for the Analysis of Complexity and Entropy in Physiological Signals
Authors: Mayor David , Panday Deepak , Kandel Hari Kala , Steffert Tony , Banks Duncan
Published: March 2021

Background: We developed CEPS as an open access MATLAB® GUI (graphical user interface) for the analysis of Complexity and Entropy in Physiological Signals (CEPS), and demonstrate its use with an example data set that shows the effects of paced breathing (PB) on variability of heart, pulse and respiration rates. CEPS is also sufficiently adaptable to be used for other time series physiological data such as EEG (electroencephalography), postural sway or temperature measurements. Methods: Data were collected from a convenience sample of nine healthy adults in a pilot for a larger study investigating the effects on vagal tone of breathing paced at various different rates, part of a development programme for a home training stress reduction system. Results: The current version of CEPS focuses on those complexity and entropy measures that appear most frequently in the literature, together with some recently introduced entropy measures which may have advantages over those that are more established. Ten methods of estimating data complexity are currently included, and some 28 entropy measures. The GUI also includes a section for data pre-processing and standard ancillary methods to enable parameter estimation of embedding dimension m and time delay τ (‘tau’) where required. The software is freely available under version 3 of the GNU Lesser General Public License (LGPLv3) for non-commercial users. CEPS can be downloaded from Bitbucket. In our illustration on PB, most complexity and entropy measures decreased significantly in response to breathing at 7 breaths per minute, differentiating more clearly than conventional linear, time- and frequency-domain measures between breathing states. In contrast, Higuchi fractal dimension increased during paced breathing. Conclusions: We have developed CEPS software as a physiological data visualiser able to integrate state of the art techniques. The interface is designed for clinical research and has a structure designed for integrating new tools. The aim is to strengthen collaboration between clinicians and the biomedical community, as demonstrated here by using CEPS to analyse various physiological responses to paced breathing.
Application of MICMAC, Fuzzy AHP, and Fuzzy TOPSIS for Evaluation of the Maintenance Factors Affecting Sustainable Manufacturing
Authors: Jasiulewicz-Kaczmarek Małgorzata , Antosz Katarzyna , Wyczółkowski Ryszard , Mazurkiewicz Dariusz , Sun Bo , Qian Cheng , Ren Yi
Published: March 2021

This paper presents an empirical study on the impact of maintenance function on more sustainable manufacturing processes. The work methodology comprises four stages. At first, ten factors of maintenance activities from a sustainable manufacturing point of view were identified. Then, in the second stage, the matrix of crossed impact multiplications applied to a classification (MICMAC) was carried out to categorize these factors based on their influence and dependence values. In the third stage, the criteria for evaluation of maintenance factors were defined, then the fuzzy analytic hierarchy process (F-AHP) was applied to determine their relative weights. In the last stage, the results of MICMAC and F-AHP analyses were used as inputs for the fuzzy technique for order preference by similarity to ideal solution (F-TOPIS) to generate aggregate scores and selection of the most important maintenance factors that have an impact on sustainable manufacturing processes. A numerical example is provided to demonstrate the applicability of the approach. It was observed that factors “Implementation of preventive and prognostic service strategies”, “The usage of M&O data collection and processing systems”, and “Modernization of machines and devices” are the major factors that support the realization of sustainable manufacturing process challenges.
Current Research and Development Status of Corrosion Behavior of Automotive Materials in Biofuels
Authors: Shehzad Aamir , Ahmed Arslan , Quazi Moinuddin Mohammed , Jamshaid Muhammad , Ashrafur Rahman S. M. , Hassan Masjuki Haji , Javed Hafiz Muhammad Asif
Published: March 2021

The world’s need for energy is increasing with the passage of time and the substantial energy demand of the world is met by fossil fuels. Biodiesel has been considered as a replacement for fossil fuels in automotive engines. Biodiesels are advantageous because they provide energy security, they are nontoxic, renewable, economical, and biodegradable and clean sources of energy. However, there are certain disadvantages of biodiesels, including their corrosive, hygroscopic and oxidative natures. This paper provides a review of automotive materials when coming into contact with biodiesel blended fuel in terms of corrosion. Biodiesels have generally been proved to be corrosive, therefore it is important to understand the limits and extents of corrosion on different materials. Methods generally used to find and calculate corrosion have also been discussed in this paper. The reasons for the occurrence of corrosion and the subsequent problems because of corrosion have been presented. Biodiesel production can be carried out by different feedstocks and the studies which have been carried out on these biodiesels have been reviewed in this paper. A certain number of compounds form on the surface of materials because of corrosion and the mechanism behind the formation of these compounds along with the characterization techniques generally used is reviewed.
Towards an Energy Self-Sufficient Resource Recovery Facility by Improving Energy and Economic Balance of a Municipal WWTP with Chemically Enhanced Primary Treatment
Authors: Budych-Gorzna Magdalena , Szatkowska Beata , Jaroszynski Lukasz , Paulsrud Bjarne , Jankowska Ewelina , Jaroszynski Tymoteusz , Oleskowicz-Popiel Piotr
Published: March 2021

The recent trend of turning wastewater treatment plants (WWTPs) into energy self-sufficient resource recovery facilities has led to a constant search for solutions that fit into that concept. One of them is chemically enhanced primary treatment (CEPT), which provides an opportunity to increase biogas production and to significantly reduce the amount of sludge for final disposal. Laboratory, pilot, and full-scale trials were conducted for the coagulation and sedimentation of primary sludge (PS) with iron sulphate (PIX). Energy and economic balance calculations were conducted based on the obtained results. Experimental trials indicated that CEPT contributed to an increase in biogas production by 21% and to a decrease in sludge volume for final disposal by 12% weight. Furthermore, the application of CEPT may lead to a decreased energy demand for aeration by 8%. The removal of nitrogen in an autotrophic manner in the side stream leads to a further reduction in energy consumption in WWTP (up to 20%). In consequence, the modeling results showed that it would be possible to increase the energy self-sufficiency for WWTP up to 93% if CEPT is applied or even higher (up to 96%) if, additionally, nitrogen removal in the side stream is implemented. It was concluded that CEPT would reduce the operating cost by over 650,000 EUR/year for WWTP at 1,000,000 people equivalent, with a municipal wastewater input of 105,000 m3/d.