bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical Activities, Contaminations of Surgeons and Cooperation with Health Authorities in 14 Orthopedic Departments in North Italy during the Most Acute Phase of Covid-19 Pandemic
Authors: Aprato Alessandro , Guindani Nicola , Massè Alessandro , Castelli Claudio C. , Cipolla Alessandra , Antognazza Delia , Benazzo Francesco , Bove Federico , Casiraghi Alessandro , Catani Fabio , Dallari Dante , D’Apolito Rocco , Franceschini Massimo , Momoli Alberto , Ravasi Flavio , Rivera Fabrizio , Zagra Luigi , Zatti Giovanni , D’Angelo Fabio
Published: May 2021

Background: From 10 March up until 3 May 2020 in Northern Italy, the SARS-CoV-2 spread was not contained; disaster triage was adopted. The aim of the present study is to assess the impact of the COVID-19-pandemic on the Orthopedic and Trauma departments, focusing on: hospital reorganization (flexibility, workload, prevalence of COVID-19/SARS-CoV-2, standards of care); effects on staff; subjective orthopedic perception of the pandemic. Material and Methods: Data regarding 1390 patients and 323 surgeons were retrieved from a retrospective multicentric database, involving 14 major hospitals. The subjective directors’ viewpoints regarding the economic consequences, communication with the government, hospital administration and other departments were collected. Results: Surgical procedures dropped by 73%, compared to 2019, elective surgery was interrupted. Forty percent of patients were screened for SARS-CoV-2: 7% with positive results. Seven percent of the patients received medical therapy for COVID-19, and only 48% of these treated patients had positive swab tests. Eleven percent of surgeons developed COVID-19 and 6% were contaminated. Fourteen percent of the staff were redirected daily to COVID units. Communication with the Government was perceived as adequate, whilst communication with medical Authorities was considered barely sufficient. Conclusions: Activity reduction was mandatory; the screening of carriers did not seem to be reliable and urgent activities were performed with a shortage of workers and a slower workflow. A trauma network and dedicated in-hospital paths for COVID-19-patients were created. This experience provided evidence for coordinated responses in order to avoid the propagation of errors.
High-Intensity Interval Training upon Cognitive and Psychological Outcomes in Youth: A Systematic Review
Authors: Alves Ana R. , Dias Renata , Neiva Henrique P. , Marinho Daniel A. , Marques Mário C. , Sousa António C. , Loureiro Vânia , Loureiro Nuno
Published: May 2021

Development of innovative and time-efficient strategies to involve youth in physical activity is pivotal in the actual inactivity pandemic. Moreover, physical activity may improve academic performance, of great interest for educators. This present systematic review aimed to analyze the effects of high-intensity interval training (HIIT) on cognitive performance and psychological outcomes in youth. A database search (Web of Science, PubMed, Scopus, and PsycINFO) for original research articles was performed. A total of eight articles met the inclusion criteria, and the Cochrane risk of bias tool was used. The studies’ results were recalculated to determine effect sizes using Cohen’s d. Different HIIT interventions reported improvements on cognitive performance at executive function (d = 0.75, +78.56%), linguistic reasoning (d = 0.25, +7.66%), concentration (d = 0.71, +61.10%), selective attention (d = 0.81, +60.73%), non-verbal and verbal abilities (d = 0.88, +47.50%; d = 1.58, +22.61%, respectively), abstract reasoning (d = 0.75, +44.50%), spatial and numerical abilities (d = 37.19, +22.85%; d = 1.20, +8.28%, respectively), and verbal reasoning (d = 1.00, +15.71%) in youth. Regarding psychological outcomes, HIIT showed higher self-concept (d = 0.28, +8.71%) and psychological well-being in boys and girls (d = 0.73, +32.43%, d = 0.39, +11.58%, respectively). To sum up, HIIT interventions between 4–16 weeks, for 8–30 min/session, at ≥85% maximal heart rate, would provide positive effects on cognitive performance and psychological outcomes in youth.
Multifractal Characteristics Analysis Based on Slope Distribution Probability in the Yellow River Basin, China
Authors: Qin Zilong , Wang Jinxin , Lu Yan
Published: May 2021

Multifractal theory provides a reliable method for the scientific quantification of the geomorphological features of basins. However, most of the existing research has investigated small and medium-sized basins rather than complex and large basins. In this study, the Yellow River Basin and its sub-basins were selected as the research areas, and the generalized fractal dimension and multifractal spectrum were computed and analyzed with a multifractal technique based on the slope distribution probability. The results showed that the Yellow River Basin and its sub-basins exhibit clear multifractal characteristics, which indicates that the multifractal theory can be applied well to the analysis of large-scale basin geomorphological features. We also concluded that the region with the most uneven terrain is the Yellow River Downstream Basin with the “overhanging river”, followed by the Weihe River Basin, the Yellow River Mainstream Basin, and the Fenhe River Basin. Multifractal analysis can reflect the geomorphological feature information of the basins comprehensively with the generalized fractal dimension and the multifractal spectrum. There is a strong correlation between some common topographic parameters and multifractal parameters, and the correlation coefficients between them are greater than 0.8. The results provide a scientific basis for analyzing the geomorphic characteristics of large-scale basins and for the further research of the morphogenesis of the forms.
Artificial Sweeteners Negatively Regulate Pathogenic Characteristics of Two Model Gut Bacteria, E. coli and E. faecalis
Authors: Shil Aparna , Chichger Havovi
Published: May 2021

Artificial sweeteners (AS) are synthetic sugar substitutes that are commonly consumed in the diet. Recent studies have indicated considerable health risks which links the consumption of AS with metabolic derangements and gut microbiota perturbations. Despite these studies, there is still limited data on how AS impacts the commensal microbiota to cause pathogenicity. The present study sought to investigate the role of commonly consumed AS on gut bacterial pathogenicity and gut epithelium-microbiota interactions, using models of microbiota (Escherichia coli NCTC10418 and Enterococcus faecalis ATCC19433) and the intestinal epithelium (Caco-2 cells). Model gut bacteria were exposed to different concentrations of the AS saccharin, sucralose, and aspartame, and their pathogenicity and changes in interactions with Caco-2 cells were measured using in vitro studies. Findings show that sweeteners differentially increase the ability of bacteria to form a biofilm. Co-culture with human intestinal epithelial cells shows an increase in the ability of model gut bacteria to adhere to, invade and kill the host epithelium. The pan-sweet taste inhibitor, zinc sulphate, effectively blocked these negative impacts. Since AS consumption in the diet continues to increase, understanding how this food additive affects gut microbiota and how these damaging effects can be ameliorated is vital.
Neural Network Approaches for Mobile Spectroscopic Gamma-Ray Source Detection
Authors: Bilton Kyle J. , Joshi Tenzing H. Y. , Bandstra Mark S. , Curtis Joseph C. , Hellfeld Daniel , Vetter Kai
Published: May 2021

Artificial neural networks (ANNs) for performing spectroscopic gamma-ray source identification have been previously introduced, primarily for applications in controlled laboratory settings. To understand the utility of these methods in scenarios and environments more relevant to nuclear safety and security, this work examines the use of ANNs for mobile detection, which involves highly variable gamma-ray background, low signal-to-noise ratio measurements, and low false alarm rates. Simulated data from a 2” × 4” × 16” NaI(Tl) detector are used in this work for demonstrating these concepts, and the minimum detectable activity (MDA) is used as a performance metric in assessing model performance.In addition to examining simultaneous detection and identification, binary spectral anomaly detection using autoencoders is introduced in this work, and benchmarked using detection methods based on Non-negative Matrix Factorization (NMF) and Principal Component Analysis (PCA). On average, the autoencoder provides a 12% and 23% improvement over NMF- and PCA-based detection methods, respectively. Additionally, source identification using ANNs is extended to leverage temporal dynamics by means of recurrent neural networks, and these time-dependent models outperform their time-independent counterparts by 17% for the analysis examined here. The paper concludes with a discussion on tradeoffs between the ANN-based approaches and the benchmark methods examined here.
Free Appropriate Public Education, the U.S. Supreme Court, and Developing and Implementing Individualized Education Programs
Authors: Rozalski Michael , Yell Mitchell L. , Warner Jacob
Published: May 2021

In 1975, the Education for All Handicapped Children Act (renamed the Individuals with Disabilities Education Act in 1990) established the essential obligation of special education law, which is to develop a student’s individualized special education program that enables them to receive a free appropriate public education (FAPE). FAPE was defined in the federal law as special education and related services that: (a) are provided at public expense, (b) meet the standards of the state education agency, (c) include preschool, elementary, or secondary education, and (d) are provided in conformity with a student’s individualized education program (IEP). Thus, the IEP is the blueprint of an individual student’s FAPE. The importance of FAPE has been shown in the number of disputes that have arisen over the issue. In fact 85% to 90% of all special education litigation involves disagreements over the FAPE that students receive. FAPE issues boil down to the process and content of a student’s IEP. In this article, we differentiate procedural (process) and substantive (content) violations and provide specific guidance on how to avoid both process and content errors when drafting and implementing students’ IEPs.
Nano Zero Valent Iron (nZVI) as an Amendment for Phytostabilization of Highly Multi-PTE Contaminated Soil
Authors: Radziemska Maja , Gusiatin Zygmunt M. , Holatko Jiri , Hammerschmiedt Tereza , Głuchowski Andrzej , Mizerski Andrzej , Jaskulska Iwona , Baltazar Tivadar , Kintl Antonin , Jaskulski Dariusz , Brtnicky Martin
Published: May 2021

In recent years, a lot of attention has been given to searching for new additives which will effectively facilitate the process of immobilizing contaminants in the soil. This work considers the role of the enhanced nano zero valent iron (nZVI) strategy in the phytostabilization of soil contaminated with potentially toxic elements (PTEs). The experiment was carried out on soil that was highly contaminated with PTEs derived from areas in which metal waste had been stored for many years. The plants used comprised a mixture of grasses—Lolium perenne L. and Festuca rubra L. To determine the effect of the nZVI on the content of PTEs in soil and plants, the samples were analyzed using flame atomic absorption spectrometry (FAAS). The addition of nZVI significantly increased average plant biomass (38%), the contents of Cu (above 2-fold), Ni (44%), Cd (29%), Pb (68%), Zn (44%), and Cr (above 2-fold) in the roots as well as the soil pH. The addition of nZVI, on the other hand, was most effective in reducing the Zn content of soil when compared to the control series. Based on the investigations conducted, the application of nZVI to soil highly contaminated with PTEs is potentially beneficial for the restoration of polluted lands.
About the Role of Interfaces on the Fatigue Crack Propagation in Laminated Metallic Composites
Authors: Pohl Philip Manuel , Kümmel Frank , Schunk Christopher , Serrano-Munoz Itziar , Markötter Henning , Göken Mathias , Höppel Heinz Werner
Published: May 2021

The influence of gradients in hardness and elastic properties at interfaces of dissimilar materials in laminated metallic composites (LMCs) on fatigue crack propagation is investigated experimentally for three different LMC systems: Al/Al-LMCs with dissimilar yield stress and Al/Steel-LMCs as well as Al/Ti/Steel-LMCs with dissimilar yield stress and Young’s modulus, respectively. The damage tolerant fatigue behavior in Al/Al-LMCs with an alternating layer structure is enhanced significantly compared to constituent monolithic materials. The prevalent toughening mechanisms at the interfaces are identified by microscopical methods and synchrotron X-ray computed tomography. For the soft/hard transition, crack deflection mechanisms at the vicinity of the interface are observed, whereas crack bifurcation mechanisms can be seen for the hard/soft transition. The crack propagation in Al/Steel-LMCs was studied conducting in-situ scanning electron microscope (SEM) experiments in the respective low cycle fatigue (LCF) and high cycle fatigue (HCF) regimes of the laminate. The enhanced resistance against crack propagation in the LCF regime is attributed to the prevalent stress redistribution, crack deflection, and crack bridging mechanisms. The fatigue properties of different Al/Ti/Steel-LMC systems show the potential of LMCs in terms of an appropriate selection of constituents in combination with an optimized architecture. The results are also discussed under the aspect of tailored lightweight applications subjected to cyclic loading.
Carboxin and Diuron Adsorption Mechanism on Sunflower Husks Biochar and Goethite in the Single/Mixed Pesticide Solutions
Authors: Szewczuk-Karpisz Katarzyna , Tomczyk Agnieszka , Celińska Magdalena , Sokołowska Zofia , Kuśmierz Marcin
Published: May 2021

The study focused on the adsorption mechanism of two selected pesticides: carboxin and diuron, on goethite and biochar, which were treated as potential compounds of mixed adsorbent. The authors also prepared a simple mixture of goethite and biochar and performed adsorption measurements on this material. The adsorbents were characterized by several methods, inter alia, nitrogen adsorption/desorption, Boehm titration, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The adsorption study included kinetics and equilibrium measurements, in the solution containing one or two pesticides simultaneously. The adsorption data were fitted to selected theoretical models (e.g., Langmuir, Freudlich, Redlich–Peterson, pseudo first-order and pseudo second-order equations). Based on the obtained results, it was stated that, among all tested adsorbents, biochar had the highest adsorption capacity relative to both carboxin and diuron. It equaled 0.64 and 0.52 mg/g, respectively. Experimental data were best fitted to the pseudo second-order and Redlich–Peterson models. In the mixed systems, the adsorption levels observed on biochar, goethite and their mixture were higher for diuron and lower for carboxin, compared to those noted in the single solutions. The presented results may enable the development of new mixed adsorbent for remediation of soils polluted with pesticides.
Carbon Nanomaterials for Enhancing the Thermal, Physical and Rheological Properties of Asphalt Binders
Authors: Li Zhelun , Yu Xin , Liang Yangshi , Wu Shaopeng
Published: May 2021

Effective thermal conduction modification in asphalt binders is beneficial to reducing pavement surface temperature and relieving the urban heat island (UHI) effect in the utilization of solar harvesting and snow melting pavements. This study investigated the performance of two nanometer-sized modifiers, graphene (Gr) and carbon nanotubes (CNTs), on enhancing the thermal, physical and rheological properties of asphalt binders. Measurements depending on a transient plant source method proved that both Gr and CNTs linearly increased the thermal conductivity and thermal diffusivity of asphalt binders, and while 5% Gr by volume of matrix asphalt contributed to 300% increments, 5% CNTs increased the two parameters of asphalt binders by nearly 72% at 20 °C. Meanwhile, a series of empirical and rheological properties experiments were conducted. The results demonstrated the temperature susceptibility reduction and high-temperature properties promotion of asphalt binders by adding Gr or CNTs. The variation trends in the anti-cracking properties of asphalt binders modified by Gr and CNTs with the modifier content differed at low temperatures, which may be due to the unique nature of Gr. In conclusion, Gr, whose optimal content is 3% by volume of matrix asphalt, provides superior application potential for solar harvesting and snow melting pavements in comparison to CNTs due to its comprehensive contributions to thermal properties, construction feasibility, high-temperature performance and low-temperature performance of asphalt binders.
Pyrolytic Conversion of Plastic Waste to Value-Added Products and Fuels: A Review
Authors: Papari Sadegh , Bamdad Hanieh , Berruti Franco
Published: May 2021

Plastic production has been rapidly growing across the world and, at the end of their use, many of the plastic products become waste disposed of in landfills or dispersed, causing serious environmental and health issues. From a sustainability point of view, the conversion of plastic waste to fuels or, better yet, to individual monomers, leads to a much greener waste management compared to landfill disposal. In this paper, we systematically review the potential of pyrolysis as an effective thermochemical conversion method for the valorization of plastic waste. Different pyrolysis types, along with the influence of operating conditions, e.g., catalyst types, temperature, vapor residence time, and plastic waste types, on yields, quality, and applications of the cracking plastic products are discussed. The quality of pyrolysis plastic oil, before and after upgrading, is compared to conventional diesel fuel. Plastic oil yields as high as 95 wt.% can be achieved through slow pyrolysis. Plastic oil has a heating value approximately equivalent to that of diesel fuel, i.e., 45 MJ/kg, no sulfur, a very low water and ash content, and an almost neutral pH, making it a promising alternative to conventional petroleum-based fuels. This oil, as-is or after minor modifications, can be readily used in conventional diesel engines. Fast pyrolysis mainly produces wax rather than oil. However, in the presence of a suitable catalyst, waxy products further crack into oil. Wax is an intermediate feedstock and can be used in fluid catalytic cracking (FCC) units to produce fuel or other valuable petrochemical products. Flash pyrolysis of plastic waste, performed at high temperatures, i.e., near 1000 °C, and with very short vapor residence times, i.e., less than 250 ms, can recover up to 50 wt.% ethylene monomers from polyethylene waste. Alternatively, pyrolytic conversion of plastic waste to olefins can be performed in two stages, with the conversion of plastic waste to plastic oil, followed by thermal cracking of oil to monomers in a second stage. The conversion of plastic waste to carbon nanotubes, representing a higher-value product than fuel, is also discussed in detail. The results indicate that up to 25 wt.% of waste plastic can be converted into carbon nanotubes.
High-Temperature Oxidation of High-Entropic Alloys: A Review
Authors: Veselkov Sergey , Samoilova Olga , Shaburova Nataliya , Trofimov Evgeny
Published: May 2021

Over the past few years, interest in high-entropic alloys (HEAs) has been growing. A large body of research has been undertaken to study aspects such as the microstructure features of HEAs of various compositions, the effect of the content of certain elements on the mechanical properties of HEAs, and, of course, special properties such as heat resistance, corrosion resistance, resistance to irradiation with high-energy particles, magnetic properties, etc. However, few works have presented results accumulated over several years, which can complicate the choice of directions for further research. This review article presents the results of studies of the mechanisms of high-temperature oxidation of HEAs of systems: Al-Co-Cr-Fe-Ni, Mn-Co-Cr-Fe-Ni, refractory HEAs. An analysis made it possible to systematize the features of high-temperature oxidation of HEAs and propose new directions for the development of heat-resistant HEAs. The presented information may be useful for assessing the possibility of the practical application of HEAs in the aerospace industry, in nuclear and chemical engineering, and in new areas of energy.
One-Dimensional (1D) Nanostructured Materials for Energy Applications
Authors: Machín Abniel , Fontánez Kenneth , Arango Juan C. , Ortiz Dayna , De León Jimmy , Pinilla Sergio , Nicolosi Valeria , Petrescu Florian I. , Morant Carmen , Márquez Francisco
Published: May 2021

At present, the world is at the peak of production of traditional fossil fuels. Much of the resources that humanity has been consuming (oil, coal, and natural gas) are coming to an end. The human being faces a future that must necessarily go through a paradigm shift, which includes a progressive movement towards increasingly less polluting and energetically viable resources. In this sense, nanotechnology has a transcendental role in this change. For decades, new materials capable of being used in energy processes have been synthesized, which undoubtedly will be the cornerstone of the future development of the planet. In this review, we report on the current progress in the synthesis and use of one-dimensional (1D) nanostructured materials (specifically nanowires, nanofibers, nanotubes, and nanorods), with compositions based on oxides, nitrides, or metals, for applications related to energy. Due to its extraordinary surface–volume relationship, tunable thermal and transport properties, and its high surface area, these 1D nanostructures have become fundamental elements for the development of energy processes. The most relevant 1D nanomaterials, their different synthesis procedures, and useful methods for assembling 1D nanostructures in functional devices will be presented. Applications in relevant topics such as optoelectronic and photochemical devices, hydrogen production, or energy storage, among others, will be discussed. The present review concludes with a forecast on the directions towards which future research could be directed on this class of nanostructured materials.
Design of Wideband Bandpass Filter Based on Corrugated Disk Resonator with Multiple Resonant Modes
Authors: Yang Qian , Liu Shuangyang , Shi Hongyu , Xu Kai-Da , Dai Xinyue , Du Hao , Zhang Anxue
Published: May 2021

A corrugated disk resonator with eight grooves is proposed for wideband bandpass filter (BPF) design. Due to the spoof localized surface plasmons resonances of the corrugated metallic structure, the dipole, quadrupole, hexapole modes, and a fundamental mode excited by the introduced short-circuited via holes are employed to realize four transmission poles (TPs) in the passband. The theoretical analysis is described by the electric field and current distributions on the resonator. The resonant frequencies can be tuned easily by the parameters of the structure, which can be used to adjust the center frequency and bandwidth of the BPF freely. Furthermore, two resonators are cascaded to obtain eight TPs to improve the selectivity performance. Finally, three fabricated filters demonstrate the design method.
On the Boundary Value Problems of Hadamard Fractional Differential Equations of Variable Order via Kuratowski MNC Technique
Authors: Refice Ahmed , Souid Mohammed Said , Stamova Ivanka
Published: May 2021

In this manuscript, we examine both the existence and the stability of solutions of the boundary value problems of Hadamard-type fractional differential equations of variable order. New outcomes are obtained in this paper based on the Darbo’s fixed point theorem (DFPT) combined with Kuratowski measure of noncompactness (KMNC). We construct an example to illustrate the validity of the observed results.
Treatment of Multidrug-Resistant and Extensively Drug-Resistant Tuberculosis in Children: The Role of Bedaquiline and Delamanid
Authors: Pecora Francesco , Dal Canto Giulia , Veronese Piero , Esposito Susanna
Published: May 2021

Multidrug-resistant (MDR) tuberculosis (TB) has been emerging at an alarming rate over the last few years. It has been estimated that about 3% of all pediatric TB is MDR, meaning about 30,000 cases each year. Although most children with MDR-TB can be successfully treated, up to five years ago effective treatment was associated with a high incidence of severe adverse effects and patients with extensively drug-resistant (XDR) TB had limited treatment options and no standard regimen. The main objective of this manuscript is to discuss our present knowledge of the management of MDR- and XDR-TB in children, focusing on the characteristics and available evidence on the use of two promising new drugs: bedaquiline and delamanid. PubMed was used to search for all of the studies published up to November 2020 using key words such as “bedaquiline” and “delamanid” and “children” and “multidrug-resistant tuberculosis” and “extensively drug-resistant tuberculosis”. The search was limited to articles published in English and providing evidence-based data. Although data on pediatric population are limited and more studies are needed to confirm the efficacy and safety of bedaquiline and delamanid, their use in children with MDR-TB/XDR-TB appears to have good tolerability and efficacy. However, more evidence on these new anti-TB drugs is needed to better guide their use in children in order to design effective shorter regimens and reduce adverse effects, drug interactions, and therapeutic failure.
Neuroprotective Effects and Mechanisms of Procyanidins In Vitro and In Vivo
Authors: Chen Juan , Chen Yixuan , Zheng Yangfan , Zhao Jiawen , Yu Huilin , Zhu Jiajin , Li Duo
Published: May 2021

This study evaluated the neuroprotective effects and mechanisms of procyanidins (PCs). In vitro, rat pheochromocytoma cells (PC12 cells) were exposed to PCs (1, 2 or 4 μg/mL) or N-Acetyl-L-cysteine (NAC) (20 μM) for 24 h, and then incubated with 200 μM of H2O2 for 24 h. Compared with H2O2 alone, PCs significantly increased antioxidant activities (e.g., glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT)), decreased levels of reactive oxygen species (ROS) and malondialdehyde (MDA), and increased nuclear factor-erythroid 2-related factor 2 (Nrf2) accumulation and increased the expression of quinone oxidoreductase 1 (NQO1), heme oxygenase 1 (HO-1), glutamate-cysteine ligase modifier subunit (GCLM), and glutamate-cysteine ligase catalytic subunit (GCLC). In vivo, zebrafish larvae (AB strain) 3 days post-fertilization (dpf) were exposed to NAC (30 μM) or PCs (4, 8 or 16 μg/mL) in the absence or presence of 300 μM of H2O2 for 4 days. Compared with H2O2 alone, PCs enhanced antioxidant activities (e.g., GSH-Px, CAT, and SOD), decreased levels of ROS and MDA, and enhanced Nrf2/ antioxidant response element (ARE) activation and raised expression levels of NQO1, HO-1, GCLM, and GCLC. In conclusion, these results indicated that PCs exerted neuroprotective effects via activating the Nrf2/ARE pathway and alleviating oxidative damage.
The Association between Nutritional Status and In-Hospital Mortality among Patients with Heart Failure—A Result of the Retrospective Nutritional Status Heart Study 2 (NSHS2)
Authors: Czapla Michał , Juárez-Vela Raúl , Łokieć Katarzyna , Karniej Piotr
Published: May 2021

Background: A nutritional status is related to the prognosis and length of hospitalisation of patients with heart failure (HF). This study aims to assess the effect of nutritional status on in-hospital mortality in patients with heart failure. Methods: We conducted a retrospective study and analysis of medical records of 1056 patients admitted to the cardiology department of the University Clinical Hospital in Wroclaw (Poland). Results: A total of 1056 individuals were included in the analysis. A total of 5.5% of patients died during an in-hospital stay. It was found that in the sample group, 25% of patients who died had a BMI (body mass index) within the normal range, 6% were underweight, 47% were overweight, and 22% were obese. Our results show that non-survivors have a significantly higher nutrition risk screening (NRS) ≥3 (21% vs. 3%; p < 0.001); NYHA (New York Heart Association) grade 4 (70% vs. 24%; p < 0.001). The risk of death was lower in obese patients (HR = 0.51; p = 0.028) and those with LDL (low-density lipoprotein) levels from 116 to <190 mg/dL (HR = 0.10; p = 0.009, compared to those with LDL <55 mg/dL). The risk of death was higher in those with NRS (nutritional risk score) score ≥3 (HR = 2.31; p = 0.014), HFmrEF fraction (HR = 4.69; p < 0.001), and LDL levels > 190 mg/dL (HR = 3.20; p = 0.038). Conclusion: The malnutrition status correlates with an increased risk of death during hospitalisation. Higher TC (total cholesterol) level were related to a lower risk of death, which may indicate the “lipid paradox”. Higher BMI results were related to a lower risk of death, which may indicate the “obesity paradox”.
Pre- and Post-Surgical Nutrition for Preservation of Muscle Mass, Strength, and Functionality Following Orthopedic Surgery
Authors: Hirsch Katie R. , Wolfe Robert R. , Ferrando Arny A.
Published: May 2021

Nutritional status is a strong predictor of postoperative outcomes and is recognized as an important component of surgical recovery programs. Adequate nutritional consumption is essential for addressing the surgical stress response and mitigating the loss of muscle mass, strength, and functionality. Especially in older patients, inadequate protein can lead to significant muscle atrophy, leading to a loss of independence and increased mortality risk. Current nutritional recommendations for surgery primarily focus on screening and prevention of malnutrition, pre-surgical fasting protocols, and combating post-surgical insulin resistance, while recommendations regarding macronutrient composition and timing around surgery are less established. The goal of this review is to highlight oral nutrition strategies that can be implemented leading up to and following major surgery to minimize atrophy and the resultant loss of functionality. The role of carbohydrate and especially protein/essential amino acids in combating the surgical stress cascade and supporting recovery are discussed. Practical considerations for nutrient timing to maximize oral nutritional intake, especially during the immediate pre- and post- surgical periods, are also be discussed.
Association of Zinc Deficiency with Development of CVD Events in Patients with CKD
Authors: Nakatani Shinya , Mori Katsuhito , Shoji Tetsuo , Emoto Masanori
Published: May 2021

Deficiency of the micronutrient zinc is common in patients with chronic kidney disease (CKD). The aim of this review is to summarize evidence presented in literature for consolidation of current knowledge regarding zinc status in CKD patients, including those undergoing hemodialysis. Zinc deficiency is known to be associated with various risk factors for cardiovascular disease (CVD), such as increased blood pressure, dyslipidemia, type 2 diabetes mellitus, inflammation, and oxidative stress. Zinc may protect against phosphate-induced arterial calcification by suppressing activation of nuclear factor kappa light chain enhancer of activated B. Serum zinc levels have been shown to be positively correlated with T50 (shorter T50 indicates higher calcification propensity) in patients with type 2 diabetes mellitus as well as those with CKD. Additionally, higher intake of dietary zinc was associated with a lower risk of severe abdominal aortic calcification. In hemodialysis patients, the beneficial effects of zinc supplementation in relation to serum zinc and oxidative stress levels was demonstrated in a meta-analysis of 15 randomized controlled trials. Thus, evidence presented supports important roles of zinc regarding antioxidative stress and suppression of calcification and indicates that zinc intake/supplementation may help to ameliorate CVD risk factors in CKD patients.
Methylation and Expression of FTO and PLAG1 Genes in Childhood Obesity: Insight into Anthropometric Parameters and Glucose–Lipid Metabolism
Authors: Czogała Wojciech , Czogała Małgorzata , Strojny Wojciech , Wątor Gracjan , Wołkow Paweł , Wójcik Małgorzata , Bik Multanowski Mirosław , Tomasik Przemysław , Wędrychowicz Andrzej , Kowalczyk Wojciech , Miklusiak Karol , Łazarczyk Agnieszka , Hałubiec Przemysław , Skoczeń Szymon
Published: May 2021

The occurrence of childhood obesity is influenced by both genetic and epigenetic factors. FTO (FTO alpha-ketoglutarate dependent dioxygenase) is a gene of well-established connection with adiposity, while a protooncogene PLAG1 (PLAG1 zinc finger) has been only recently linked to this condition. We performed a cross-sectional study on a cohort of 16 obese (aged 6.6–17.7) and 10 healthy (aged 11.4–16.9) children. The aim was to evaluate the relationship between methylation and expression of the aforementioned genes and the presence of obesity as well as alterations in anthropometric measurements (including waist circumference (WC), body fat (BF_kg) and body fat percent (BF_%)), metabolic parameters (lipid profile, blood glucose and insulin levels, presence of insulin resistance) and blood pressure. Expression and methylation were measured in peripheral blood mononuclear cells using a microarray technique and a method based on restriction enzymes, respectively. Multiple regression models were constructed to adjust for the possible influence of age and sex on the investigated associations. We showed significantly increased expression of the FTO gene in obese children and in patients with documented insulin resistance. Higher FTO expression was also associated with an increase in WC, BF_kg, and BF_% as well as higher fasting concentration of free fatty acids (FFA). FTO methylation correlated positively with WC and BF_kg. Increase in PLAG1 expression was associated with higher BF%. Our results indicate that the FTO gene is likely to play an important role in the development of childhood adiposity together with coexisting impairment of glucose-lipid metabolism.
Anti-Fatigue Effect of a Dietary Supplement from the Fermented By-Products of Taiwan Tilapia Aquatic Waste and Monostroma nitidum Oligosaccharide Complex
Authors: Chen Ying-Ju , Kuo Chun-Yen , Kong Zwe-Ling , Lai Chin-Ying , Chen Guan-Wen , Yang An-Jen , Lin Liang-Hung , Wang Ming-Fu
Published: May 2021

The Taiwan Tilapia is an important aquaculture product in Taiwan. The aquatic by-products generated during Tilapia processing, such as fish bones and skin, are rich in minerals and protein. We aimed to explore the effect of a dietary supplement, comprising a mixture of fermented Tilapia by-products and Monostroma nitidum oligosaccharides as the raw materials, combined with physical training on exercise performance and fatigue. We used a mouse model that displays a phenotype of accelerated aging. Male senescence-accelerated mouse prone-8 (SAMP8) mice were divided into two control groups—with or without physical training—and supplemented with different doses (0.5 times: 412 mg/kg body weight (BW)/day; 1 time: 824 mg/kg BW/day; 2 times: 1648 mg/kg BW/day) of fermented Tilapia by-products and Monostroma nitidum oligosaccharide-containing mixture and combined with exercise training groups. Exercise performance was determined by testing forelimb grip strength and with a weight-bearing exhaustive swimming test. Animals were sacrificed to collect physical fatigue-related biomarkers. Mice dosed at 824 or 1648 mg/kg BW/day showed improvement in their exercise performance (p < 0.05). In terms of biochemical fatigue indicators, supplementation of 824 or 1648 mg/kg BW/day doses of test substances could effectively reduce blood urea nitrogen concentration and lactate concentration and increase the lactate ratio (p < 0.05) and liver glycogen content post-exercise (p < 0.05). Based on the above results, the combination of physical training and consumption of a dietary supplementation mixture of fermented Tilapia by-products and Monostroma nitidum oligosaccharides could improve the exercise performance of mice and help achieve an anti-fatigue effect.
Dietary Habits before and during the COVID-19 Epidemic in Selected European Countries
Authors: Skotnicka Magdalena , Karwowska Kaja , Kłobukowski Filip , Wasilewska Eliza , Małgorzewicz Sylwia
Published: May 2021

During the so-called “second wave of the pandemic” in Europe, the authors conducted a cross-sectional online survey that aimed to examine changes in dietary habits and associated practices, as well as physical activity during the COVID-19 pandemic and before the onset of lockdowns in three European countries: Poland, Austria and the United Kingdom. Methods: The online observational study, both prospective and retrospective, conducted with the use of social media for the distribution of an anonymous online questionnaire, was completed from 1 October to 30 October 2020, during the second wave of the pandemic in Europe. The study encompassed a total of 1071 adults from Poland (n = 407), Austria (n = 353) and the United Kingdom (n = 311). Results: The results of this study indicate that the COVID-19 confinement period influenced eating behavior and the level of physical activity in a group of adult residents of Poland, Austria and the United Kingdom. The general shopping frequency decreased, regardless of the place and manner. However, there was an increased interest in online grocery shopping. The resulting data revealed an increased frequency of the daily consumption of food products such as dairy, grains, fats, vegetables and sweets (p < 0.05). A rise in the frequency of purchasing frozen goods and food with long shelf life has also been observed. The changed workplace and working conditions or unemployment probably affected a perceptible rise in alcohol consumption (p = 0.02). In turn, physical activity levels markedly decreased, which reflected the body mass changes. Conclusion: The dietary habits in the studied countries have changed as a result of the pandemic situation. They contribute to the aggravation of the problem of excess body weight and its health consequences.
Statin as a Potential Chemotherapeutic Agent: Current Updates as a Monotherapy, Combination Therapy, and Treatment for Anti-Cancer Drug Resistance
Authors: Tilija Pun Nirmala , Jeong Chul-Ho
Published: May 2021

Cancer is incurable because progressive phenotypic and genotypic changes in cancer cells lead to resistance and recurrence. This indicates the need for the development of new drugs or alternative therapeutic strategies. The impediments associated with new drug discovery have necessitated drug repurposing (i.e., the use of old drugs for new therapeutic indications), which is an economical, safe, and efficacious approach as it is emerged from clinical drug development or may even be marketed with a well-established safety profile and optimal dosing. Statins are inhibitors of HMG-CoA reductase in cholesterol biosynthesis and are used in the treatment of hypercholesterolemia, atherosclerosis, and obesity. As cholesterol is linked to the initiation and progression of cancer, statins have been extensively used in cancer therapy with a concept of drug repurposing. Many studies including in vitro and in vivo have shown that statin has been used as monotherapy to inhibit cancer cell proliferation and induce apoptosis. Moreover, it has been used as a combination therapy to mediate synergistic action to overcome anti-cancer drug resistance as well. In this review, the recent explorations are done in vitro, in vivo, and clinical trials to address the action of statin either single or in combination with anti-cancer drugs to improve the chemotherapy of the cancers were discussed. Here, we discussed the emergence of statin as a lipid-lowering drug; its use to inhibit cancer cell proliferation and induction of apoptosis as a monotherapy; and its use in combination with anti-cancer drugs for its synergistic action to overcome anti-cancer drug resistance. Furthermore, we discuss the clinical trials of statins and the current possibilities and limitations of preclinical and clinical investigations.
Descriptors of Cytochrome Inhibitors and Useful Machine Learning Based Methods for the Design of Safer Drugs
Authors: Beck Tyler C. , Beck Kyle R. , Morningstar Jordan , Benjamin Menny M. , Norris Russell A.
Published: May 2021

Roughly 2.8% of annual hospitalizations are a result of adverse drug interactions in the United States, representing more than 245,000 hospitalizations. Drug–drug interactions commonly arise from major cytochrome P450 (CYP) inhibition. Various approaches are routinely employed in order to reduce the incidence of adverse interactions, such as altering drug dosing schemes and/or minimizing the number of drugs prescribed; however, often, a reduction in the number of medications cannot be achieved without impacting therapeutic outcomes. Nearly 80% of drugs fail in development due to pharmacokinetic issues, outlining the importance of examining cytochrome interactions during preclinical drug design. In this review, we examined the physiochemical and structural properties of small molecule inhibitors of CYPs 3A4, 2D6, 2C19, 2C9, and 1A2. Although CYP inhibitors tend to have distinct physiochemical properties and structural features, these descriptors alone are insufficient to predict major cytochrome inhibition probability and affinity. Machine learning based in silico approaches may be employed as a more robust and accurate way of predicting CYP inhibition. These various approaches are highlighted in the review.
DNA Aptamers against Vaccinia-Related Kinase (VRK) 1 Block Proliferation in MCF7 Breast Cancer Cells
Authors: Carrión-Marchante Rebeca , Frezza Valerio , Salgado-Figueroa Ana , Pérez-Morgado M. Isabel , Martín M. Elena , González Víctor M.
Published: May 2021

Vaccinia-related kinase (VRK) 1 is a serin/threonine kinase that plays an important role in DNA damage response (DDR), phosphorylating some proteins involved in this process such as 53BP1, NBS1 or H2AX, and in the cell cycle progression. In addition, VRK1 is overexpressed in many cancer types and its correlation with poor prognosis has been determined, showing VRK1 as a new therapeutic target in oncology. Using in vitro selection, high-affinity DNA aptamers to VRK1 were selected from a library of ssDNA. Selection was monitored using the enzyme-linked oligonucleotide assay (ELONA), and the selected aptamer population was cloned and sequenced. Three aptamers were selected and characterized. These aptamers recognized the protein kinase VRK1 with an affinity in the nanomolar range and showed a high sensibility. Moreover, the treatment of the MCF7 breast cell line with these aptamers resulted in a decrease in cyclin D1 levels, and an inhibition of cell cycle progression by G1 phase arrest, which induced apoptosis in cells. These results suggest that these aptamers are specific inhibitors of VRK1 that might be developed as potential drugs for the treatment of cancer.
Oleacein Intestinal Permeation and Metabolism in Rats Using an In Situ Perfusion Technique
Authors: López-Yerena Anallely , Pérez Maria , Vallverdú-Queralt Anna , Miliarakis Eleftherios , Lamuela-Raventós Rosa M. , Escribano-Ferrer Elvira
Published: May 2021

Oleacein (OLEA) is one of the most important phenolic compounds in extra virgin olive oil in terms of concentration and health-promoting properties, yet there are insufficient data on its absorption and metabolism. Several non-human models have been developed to assess the intestinal permeability of drugs, among them, single-pass intestinal perfusion (SPIP), which is commonly used to investigate the trans-membrane transport of drugs in situ. In this study, the SPIP model and simultaneous luminal blood sampling were used to study the absorption and metabolism of OLEA in rats. Samples of intestinal fluid and mesenteric blood were taken at different times and the ileum segment was excised at the end of the experiment for analysis by LC–ESI–LTQ–Orbitrap–MS. OLEA was mostly metabolized by phase I reactions, undergoing hydrolysis and oxidation, and metabolite levels were much higher in the plasma than in the lumen. The large number of metabolites identified and their relatively high abundance indicates an important intestinal first-pass effect during absorption. According to the results, OLEA is well absorbed in the intestine, with an intestinal permeability similar to that of the highly permeable model compound naproxen. No significant differences were found in the percentage of absorbed OLEA and naproxen (48.98 ± 12.27% and 43.96 ± 7.58%, respectively).
Photodynamic Therapy in Orthodontics: A Literature Review
Authors: Olek Marcin , Machorowska-Pieniążek Agnieszka , Stós Wojciech , Kalukin Janusz , Bartusik-Aebisher Dorota , Aebisher David , Cieślar Grzegorz , Kawczyk-Krupka Aleksandra
Published: May 2021

Treatment of malocclusions using fixed orthodontic appliances makes it difficult for patients to perform hygiene procedures. Insufficient removal of bacterial biofilm can cause enamel demineralization, manifesting by visible white spot lesions or periodontal diseases, such as gingivitis periodontitis or gingival hyperplasia. The classic methods of preventing the above problems include, in addition to proper hygiene, ultrasonic scaling, periodontal debridement, and oral rinses based on chlorhexidine. New alternative methods of reducing plaque around brackets are being developed. There is a growing interest among researchers in the possibility of using photodynamic therapy in orthodontics. A literature search for articles corresponding to the topic of this review was performed using the PubMed and Scopus databases and the following keywords: ‘photodynamic therapy’, ‘orthodontics’, and ‘photosensitizer(s)’. Based on the literature review, two main directions of research can be distinguished: clinical research on the use of photodynamic therapy in the prevention of white spot lesions and periodontal diseases, and ex vivo research using a modified orthodontic adhesive by adding photosensitizers to them. Methylene blue is the most frequently used photosensitizer in clinical trials. The effectiveness of antimicrobial photodynamic therapy is mainly compared to the ultrasonic scaler as a single therapy or as an adjunct to the ultrasonic scaler. In their conclusions, the researchers most often emphasize the effectiveness of antimicrobial photodynamic therapy in reducing microbial levels in patients treated with fixed appliances and the possibility of using it as an alternative to routine procedures aimed at maintaining a healthy periodontium. The authors suggest further research on the use of photodynamic therapy to prove the validity of this method in orthodontics. It should also not be forgotten that proper hygiene is the basis for maintaining oral cavity health, and its neglect is a contraindication to orthodontic treatment.