bluuebunny/crossref_metadata_2025 huggingface dataset:
A radiological study of the central circulation in the lungfish, Protopterus aethiopicus
Authors: Johansen Kjell , Hol Ragnar
Published: February 2005

The dipnoan heart is only in part structurally developed to support a separated circulation in pulmonary and systemic circuits. In the present investigation biplane angiocardiography has been used to describe the extent of such a double circulation and the factors which may modify it in the African lungfish, Protopterus aethiopicus.

Contrast injections in the pulmonary vein revealed a clear tendency for aerated blood returing from the lungs to be selectively dispatched to the anterior branchial arteries giving rise to the major systemic circulation. Contrast injections in the vena cava delineated the sinus venosus as a large receiving chamber for systemic venous blood. Contraction of the sinus venosus discharged blood into the right, posterior part of the partially divided atrial space. Contrast injection in the pulmonary vein showed that vessel to pass obliquely from right to left such that blood was emptied distinctly into the left side of the atrium. During contraction the atrial space tended to retain a residual volume in its anterior undivided part which minized mixing.

Ventricular filling occurred through separate right and left atrio‐ventricular connections. Right‐left separation in most of the ventricle was maintained by the partial ventricular septum, the trabeculated, spongelike myocardium and the mode of inflow from the atria. Mixing in the anterior undivided portion of the ventricle during the ejection phase was slight due to a streamlined ejection pattern.

The outflow through the bulbus cordis occurred in discrete streams which in part were structurally separated by well developed spiral folds. In the anterior bulbus segment the spiral folds are fused and make completely separate dorsal and ventral outflow tracts. The ventral bulbus channel provides blood to the three anterior branchial arteries. The second and third branchial arteries are large and represent direct shunts to the dorsal aorta. The fourth and fifth branchial arteries are gill bearing and receive blood form the dorsal bulbus channel. The most posterior epibranchial vessels give rise to the pulmonary arteries.
Comparative histochemical investigation of the organ of corti in the kangaroo rat, gerbil, and guinea pig
Authors: Webster Douglas B. , Stack Carol R.
Published: February 2005

The distribution of carbohydrates, proteins, and lipids was studied in the cochleae of kangaroo rat, gerbil, and guinea pig using both fixed paraffin sections and fresh‐frozen cryostat sections. Enzyme distribution in the cochleae of the three speices was studied with both EDTA‐decalcified and undecalcified fresh‐frozen cryostat sections.

Although the cochleae of the three species are morphologically different, their distributions of proteins, carbohydrates, and lipids are similar. The zona pectinata of the basilar membrane—which is hypertrophied in the kangaroo rat and gerbil but normal in the guinea pig—stains the same in all three species. The unique, flaskshaped Hensen cells of the kangaroo rat contain more protein than do the normal Hensen cells of the gerbil and guinea pig. At least some of the protein in the kangaroo rat Hensen cells is in the form of carboxylic esterases which are not affected by 10−4 M eserine, but are inhibited by 10−2 M eserine and 10−6 M E600. More than one population of carboxylic esterases is indicated by this reaction to inhibitors and by the results of enzyme distribution tests which used different substrates. A high concentration of malate dehydrogenase in the kangaroo rat Hensen cells may be related to the synthesis of carboxylic esterases. The possible role of these esterases in cochlear functioning is discussed.
Dense bodies of the contractile system of cardiac muscle in Venus mercenaria
Authors: Hayes Raymond L. , Kelly Robert E.
Published: February 2005

Dense bodies in the heart muscle of Venus mercenaria exist in two forms, free and attached. Free dense bodies morphologically consist of fascicles of thin filaments in parallel array and bound together by a dense, amorphous proteinaceous material. The binding of dense bodies to the cell membrane is effected via connecting filaments of the amorphous material of the dense body which join a condensation of morphologically similar material attached to the inner osmiophilic layer of the unit membrane. This composite of dense body, connecting filaments, membrane condensation and unit cell membrane has been termed collectively the attachment plaque. The attachment plaque is part of an extensive network on the cell surface which obligates that surface to a role in the contractile process. Moreover, this set of attachment plaques imposes an organization and an orientation to most thin filaments of the cell and preserves the contractile axis of the cell.
The ultrastructure of smooth cardiac muscle in the clam, Venus mercenaria
Authors: Kelly R. E. , Hayes R. L.
Published: February 2005

A fine structural study of the ventricular muscle of Venus mercenaria has revealed that it is an invertebrate smooth muscle. In the relaxed state induced by acetylcholine, both thick (350 Å) and thin (80 Å) myofilaments are observed. These are loosely distributed in bundles in the periphery of the mononucleated myocytes. The central core of the cell contains an ovoid nucleus, α‐glycogen rosettes, round mitochondria and numerous smooth surfaced vesicles of the endoplasmic reticulum. After exposure to serotonin, all myofilaments are compacted in the peripheral cytoplasm and become oriented parallel to the longitudinal cellular axis. This produces contraction bands visible in phase contrast microscopy. Because these myofilaments attach to the cell membrane at sites of attachment plaques, contraction of the cell results in the serial evagination or blebbing of the cell surface. The above features are clearly demonstrable in this invertebrate smooth muscle and strongly suggest a sliding filament model as the contractile mechanism in this tissue. Moreover, the cell surface is thought to play an active and major role in that process.
Morphological, ultrastructural, histochemical and electrophoretic studies on the venom system of Nasonia vitripennis walker (hymenoptera: Pteromalidae)
Authors: Ratcliffe N. A. , King P. E.
Published: February 2005

The venom system of Nasonia vitripennis is well‐developed and composed of an unbranched acid gland and associated reservoir. Fine‐structural, histochemical and electrophoretic studies indicate that the venom is produced by two protein‐secreting epithelia. The bulk of the venom is synthesised in the columnar cells of the acid gland and discharged via “vesicular organelles” and the efferent ductular system into the lumen of the reservoir. The acid gland also contains squamous chitogenous cells, situated either around the central lumen or interposed between the bases of the columnar cells. Once within the reservoir, the venom is probably activated by enzymatic secretions from the reservoir secretory cells. Each of these cells has a “vesicular organelle” but, in contrast to the columnar cells of the acid gland, the cytoplasm contains a preponderance of free ribosomes, and protein segregation apparently occurs outside the Golgi complexes.

The venom is expelled through the efferent discharge duct by muscular contractions, which open the duct lumen and bring it into contact with the funnel of the ovipositor. Excessive distortion of the duct is prevented by a massive ventral ligament.
Observations on the origin of two substances produced by the vitellaria in Gorgoderina attenuata
Authors: Koulish Sasha
Published: February 2005

The vitelline cells in Gorgoderina attenuata produce two qualitatively distinct substances. One substance assumes the form of individual, dense, osmiophilic globules. Many globules are contained in a single vesicle. The other substance is an amorphous mass of medium density that completely fills a vesicle.

Observations indicate that the dense, osmiophilic globules develop in association with a system of small, contiguous, ribosome‐free vesicles. It is suggested that this system of vesicles constitute a Golgi apparatus for these cells.

The amorphous mass substance develops in vesicles which appear to be derived from endoplasmic reticulum. Close association between the amorphous mass vesicle and mitochondria are commonly observed.
The fine structure of campaniform sensilla on the halteres of Drosophila melanogaster
Authors: Chevalier Robert L.
Published: February 2005

The campaniform sensilla on halteres of Drosophila were studied by electron microscopy in order to establish the relationships of functional elements in the sensory system. The surface of the sensillum consists of an oval cuticular cap membrane which may contain resilin, the rubberlike protein. A border of denser cuticle rings the cap membrane, and extending down around the neural process is a third type of cuticle filled with a fourth light fibrous type. The four cuticular components form a system for displacement of the neural process. The neural process is differentiated into a terminal fan‐shaped structure projecting from a bulbous dilatation which tapers to a neck region ending proximally with two basal bodies. The neural process is packed with microtubules. Surrounding the dendrite is an inner enveloping cell, attached to the basal body region by septate desmosomes and by desmosomes to which microtubules of the enveloping cell are applied. An outer enveloping cell surrounds the inner one. The tip of the neural process is covered with a dense secretion which is tightly bound to the cap membrane. The dense secretion is surrounded by an extracellular fluid which might be compressed hydraulically by the cuticular system. The stimulus of cuticular distortion could thus be transmitted to the neural process which may be displaced between its fixed ends.
Observations on the epidermis of desert‐living iguanids
Authors: Maderson P. F. A. , Mayhew W. W. , Sprague G.
Published: February 2005

The epidermis of 146 specimens of Dipsosaurus dorsalis and 182 Uma notata collected throughout the active period of the animals' year has been examined. The morphology of the epidermis is essentially similar to previously described lacertilians but differs in the relatively great degree of development of the mesos layer and the complete keratinization of the lacunar tissue prior to sloughing.

Analysis of sloughing frequency throughout the year suggests that species specific patterns may exist, but these do not correlate with any particular known ecologic datum. The patterns do not reflect the reproductive activity of the two species supporting previous experimental conclusions on the lack of effect of gonadial hormones on epidermal activity.

There appears to be no evidence of association of femoral gland activity with epidermal activity in D. dorsalis, but the situation is not clearcut in U. notata. These data are discussed in the light of recent studies of the evolutionary origin of epidermal glands in lizards.
A study of oogenesis and early embryogenesis in the rabbit, Oryctolagus cuniculus, with special reference to the structural changes of mitochondria
Authors: Anderson Everett , Condon William , Sharp Daniel
Published: February 2005

The structural changes of mitochondria that occur during oogenesis and early embryogenesis in the rabbit have been examined with the electron microscope. Mitochondria of oogonia are both elongate and oval and contain a variable number of cristae which may or may not traverse the longitudinal axis of the organelle. When oogonia differentiate into oocytes, mitochondria become spheroidal and their cristae are sparse when compared with those found in the ellipsoidal organelles of concomitantly maturing follicle cells. As differentiation proceeds, the cristae of the mitochondria display varied configurations. For example, many display an arch‐like arrangement in several regions of the organelle whereas others contain a pair of concentric membranes closely associated with limiting membrane of the mitochondrion.

Mitochondria of stages from the fertilized egg to the morula possess the same internal structure as those of young oocytes. As the morula differentiates into a blastocyst there is a gradual increase in the size of the mitochondria and an increase in the number of cristae. We believe that the number and modifications of cristae indicate stages of mitochondriogenesis and the level of enzymatic activity in which this organelle is engaged during oogenesis and early embryogenesis.
Seasonal reproductive activity in the testes and seminal vesicles of the catfish, Heteropneustes fossilis (Bloch)
Authors: Nayyar S. K. , Sundararaj Bangalore I.
Published: February 2005

A seasonal study of the seminal vesicles in relation to that of the testes had been conducted in the catfish, H. fossilis. The annual reproductive cycle of the catfish has been divided into (i) Preparatory period (February–April), (ii) Prespawning period (May–June), (iii) Spawning period (July–August) and (iv) Postspawning period (September–January). Testes exhibit initiation of spermatogenesis in the mid‐preparatory period, but significant increase in weight of the testes accompanied by active spermatogenesis occurs during the prespawning period. In the spawning period, the testes are maximally enlarged and their seminiferous tubules are packed with spermatozoa. Following spawning, the testes gradually regress in the postspawning period. The seminal vesicles show initiation of secretory activity during the preparatory period but their recrudescence lags behind that of the testes by about a month. The seminal vesicles attain maximum weight and secretory activity during the spawning period. Thereafter, the seminal vesicles regress precipitously and sooner than the testes.

The histochemical and biochemical studies on the seminal vesicles indicate that the secretion contains mucoproteins, acid mucopolysaccharides, primary proteoses, besides traces of phospholipids and native proteins.
Ultrastructural and histochemical studies on the epithelium revestment layer in the tube feet of the starfish Asterina stellifera
Authors: de Souza Santos Helena , da Silva Sasso Wilson
Published: February 2005

The “cuticle,” which revests the starfish tube foot, has been studied by electron microscopy and the findings correlated with histochemical observations.

The “cuticle” is composed by two distinct zones; an outer zone including numerous microvilli, which extend from the inner zone into and through a fibrillar substance distinctly organized in two layers. These microvilli protrude slightly beyond the outer surface, where their tips give rise tonumerous extremely delicate fibrils. The second inner zone, of quite variable thickness and condensation of material, presents a coarser fibrous matrix where organelles and inclusions can be found.

The whole cuticular complex does not derive from the majority of the epithelial cells, but is probably an extension of a special kind of T‐shaped cells appearing at intervals, the “cuticle” forming a syncytial surface.

Histochemical investigations indicate that the “cuticle” contains a combination of neutral and acid mucopolysaccharide, with a marked neutral predominance, the outer one displaying also an extremely thin coat of acid mucopolysaccharide with the sulfate group.

The ordered arrangement of the microvilli suggests that this situation is imposed by the strong bond existing between the microvilli and the ouble mucopolysaccharide layers which would act as a cementing substance stabilizing the entire apical surface of the cell.
Placentation in the garter snake, Thamnophis sirtalis
Authors: Hoffman Loren H.
Published: February 2005

The placental memebranes and uterus of the garter snake, Thamnophis sirtalis, were studied using histological, histochemical, electron microscopic, dye transfer, and radioisotopic techniques. The conceptuses are completely enclosed throughout gestation by a transparent shell membrane which is produced by glandular epithelia in the uterine segment of the oviduct.

Both chorio‐allantoic and omphalo (yolk‐sac) placentation are observed in this snake. The growth of the extra‐embryonic mesoderm takes place in a manner peculiar to placental reptiles, and results in the isolation of the omphaloplacenta from the yolk‐sac wall. On the basis of morphology, enzyme histochemistry, and phagocytosis of Trypan blue particles, this structure is interpreted as a site of histiotrophic absorption.

The chorio‐allantoic region of placentation is simple in structure. Fetal and maternal capillaries are closely apposed but always separated by layers of uterine and chorionic epithelium and the thin shell membrane. The placental membranes of the garter snake are similar in many respects to those of other live‐bearing snakes, but less specialized than most lizard placentate.

Isotopically labelled sodium and glycine are passed to the fetus following maternal injection, the latter at least apparently via the omphaloplacenta. The permeability to iron and phospate is extremely low. On the basis of these results and the selective transfer of certain dyestuffs, it appears that the shell membrane functions as a dialyzing membrane.
The fine structure of the larval firefly light organ
Authors: Peterson Margaret K.
Published: February 2005

Light organs of larval photurid fireflies, which emit their light in a steady glow, differ from those of adults, which have a precisely controlled flash pattern, in having no specialized tracheal end organs and no morphologically distinct cortical region of the photocytes. However, like the adult organs, larval lanterns are abundantly supplied with both tracheoles and nerve fibers. Each of the two abdominal light organs of the larva consists of a dorsal (“reflector”) layer and a conical ventral photogenic layer. The dorsal layer is a single layer of columnar cells containing “urate” granules, mitochondria and a rich supply of glycogen. The photogenic layer is a compact mass of photocytes, containing photocyte granules similar to those of the adult organ, many elongated mitochondria, and a vesiculated reticulum. Numerous tracheoles run between the interlocking membranes of the photocytes. A large nerve traverses the dorsal layer and enters the photogenic layer, where its fibers diverge between the photocytes. Many structures having the appearance of neurosecretory nerve endings are found among the folds of the photocyte membranes. However, no tight junctions (synapses?) were found.
The growth and differentiation of the regenerating spinal cord of the lizard, Anolis carolinensis
Authors: Egar Margaret , Simpson Sidney B. , Singer Marcus
Published: February 2005

The present work describes the ultrastructure of the spinal cord in the regenerating tail of the lizard, Anolis. The distal growing region of the tail contains the advancing ependymal tube which is relatively devoid of axons but already contains channels between ependymal cell processes which anticipate their ingrowth. More proximally, fascicles of naked axons having their origin in the stump are present in the ependymal channels. Therefore, the pattern of fiber regeneration in the spinal cord is prescribed by the ependyma and not by the growing axons. Details of the ultrastructure of proximal, intermediate, and distal regions of the regenerate are reported. Particular attention is paid to the structure and differentiation of the ependymal cells and the relation of the ependyma to other glial cells, to nerve fibers, and to meningeal tissues.
Some developmental aspects of the head skeleton of the 35–37 mm Squalus acanthias foetus
Authors: Jollie Malcolm
Published: February 2005

Two specimens of a group of five foetuses, 35–37 mm in total length, of Squalus acanthias were serially sectioned and stained. These specimens were studied for the purpose of testing statements made in the literature, especially by Holmgren ('40), regarding cell sources and skeletal development. Many of Holmgren's results were confirmed with some important differences in detail. Limited blastemas of dermal bones were not evident although a diffuse subepidermal blastema was present in certain areas. There is evidence of delamination addition to the jaws and many parts of the endocranium. Although the trabecula is in part of visceral origin, the visceral material does not represent an infrapharyngohyal since it always has been an integral part of the endocranium, never a formed element of the arch. The same applies to the pharyngo region of the hyoid arch. The pharyngohyal tissue forms the area of articulation of the hyomandibula and the lamina hypotica. Thus a typical lateral commissure is not formed and the resulting otic capsule‐basicranial association is unique to the elasmobranchs. The hyomandibula is the epihyal and there is no evidence of a symplectic in this group. The jaws appear to incorporate delamination tissue, associated in teleostomes with dermal bone, in addition to the neural crest proper.
The ontogenic development of the vertebrae in some Gekkonoid lizards
Authors: Werner Yehudah L.
Published: February 2005

The ontogeny of amphicoelous vertebrae was studied in Ptyodactylus hasselquistii and Hemidactylus turcicus, and that of procoelous vertebrae, in Sphaerodactylus argus. The embryos were assigned arbitrary stages, drawn to scale, and mostly studied in serial sections.

Resegmentation occurs as in all amniotes. A sclerocoel divides each sclerotome into an anterior “presclerotomite” and a denser posterior “postsclerotomite.” Tissue surrounding the intersegmental boundary forms the centrum, which is intersegmental. Tissue around the sclerocoel builds the intervertebral structures, which are midsegmental. In the trunk and neck, postsclerotomites form neural arches, and presclerotomites build zygapophyses.

The adult centrum consists of the perichordal primary centrum, plus neural arch bases (= secondary centrum). Between the latter and the arch proper, a neurocentral suture persists until obliterated in maturity. A dorso‐ventral central canal persists on either side of the primary centrum, between the latter and the secondary centrum.

The notochord becomes true cartilage midvertebrally in all vertebrae, and elastic cartilage intervertebrally in the posterior caudal region. Elsewhere its characteristic tissue persists.

Intervertebrally, cervical hypapophyses, caudal chevrons and chevron‐bases in the trunk are preformed early in cartilage. Directly ossifying median intercentra are added later in all regions.

The first cervical presclerotomite is absent: the hypapophysis (= corpus) of the atlas consists exclusively of postsclerotomitic tissue, there is no proatlas, and the odontoid lacks the apical half‐centrum present in other lepidosaurians.

In the autotomous caudal region presclerotomites are as prominent as postsclerotomites. Both build neural arches, the two arches of each vertebra remaining distinct and ossifying separately, so that the intersegmental autotomy split persists between them.

The last sclerotome is complete, its postsclerotomite forming a half centrum which ossifies.

In Sphaerodactylus, while the vertebrae ossify, each intervertebral ring becomes concave anteriorly, convex posteriorly; it remains as a cushion between the condyle and a facet formed by differential growth of the centra. Thus these procoelous centra resemble the amphicoelous centra of Ptyodactylus and Hemidactylus, rather than the procoelus centra of other squamates.

The vertebral column of Gekkonoidea closely resembles in its development and microscopical structure that of Sphenodon.
A quantitative electron microscope analysis of peripheral nerve in the urodele amphibian in relation to limb regenerative capacity
Authors: Egar Margaret , Singer Marcus
Published: February 2005

An approximate 1:1 ratio of myelinated to unmyelinated fibers was established in counts from electron micrograph montages in nerves of the newt, Triturus (Notophthalmus) viridescens. The number of myelinated fibers correspond to the number counted with the light microscope after osmium fixation. Light microscope counts of silver impregnated sections yielded a value slightly higher suggesting that, except for bundles of unmyelinated fibers, the silver technique revealed mainly myelinated fibers. The results were used to reassess previous quantitative studies on the relation between number of nerve fibers and the control which nerves exert on regeneration. For a truer estimate of the number of axons affecting regeneration, fiber values previously reported should now be doubled to include the large number of unmyelinated fibers. However, calculations show that the unmyelinated fibers contribute less than 3% of the total neuroplasm in the peripheral nerve.

Finally, counts made of Schwann cells and fibroblasts show that the latter are few in number.
Variations and developmental abnormalities in the sexual apparatus of the adult axolotl (Siredon mexicanum)
Authors: Brunst V. V.
Published: February 2005

The sexual apparatus was studied in 100 adult axolotls (Siredon mexicanum) for 13 different spawnings. The ages of the animals varied between two and six years. Additional material from Indiana University was also studied. Altogether there were 55 female and 52 male adult axolotls represented. The purpose of the study was to investigate the limits of the variations occurring in normal axolotls and to compare the incidences of variations and developmental abnormalities in adult animals of both sexes at various ages and belonging to different strains.

Among the 13 spawnings examined, five strains were completely normal in 100% of the animals, but the remaining eight strains all included abnormal animals. The incidence of abnormal animals in some of these latter strains was 40% or even 50%. Since all of the animals were under the same conditions, the variability and the occurrence of developmental abnormalities most likely depended upon hereditary factors. Among 55 females, only seven (12.7%) were abnormal; only four of these had developmental abnormalities, and only one was hermaphrodite. Among 45 males from the author's axolotl colony, 16 (28%) were abnormal. Of these latter, six had no sex cells or very few; this variation must be regarded as a developmental abnormality. All of these malformations resulted from major degeneration processes and abnormal morphogenesis.

Arrested development was also observed in many males. Spermatozoa were completely absent from the testes of eight animals. In the additional material from Indiana University (testes from 7 males), there was also one completely abnormal testis with major degeneration processes and complete absence of sex cells. It is evident that variability and the incidence of developmental abnormalities in the sexual apparatus in adult axolotls of some strains are very great.
Fine structure of the noctuid moth ear. I. The transducer area and connections to the tympanic membrane in Feltia subgothica haworth
Authors: Ghiradella Helen
Published: February 2005

The sensillum of the ear of Feltia subgothica contains two ciliated receptor cells, the A cells. The cilium of each is enclosed within a well developed scolops consisting of a cap, a set of scolopalial rods and a collar, an unusual structure contained within the dendrite. The tip of the cilium is inserted in a channel in the scolopalial cap.

The cap is linked to the tympanic membrane by a series of three structures: a cap cell, a microtubular shaft and a microfibrillar plug. The two latter structures are heavily reinforced by cytoskeletal elements and the microfibrillar plug is actually continuous with the tympanic membrane. These three structures transmit the vibration of the tympanic membrane to the scolops.

The simplicity and accessibility of the ear suggests that it might be a good system in which to investigate cellular events associated with transduction of sound in these receptors.
Spermatogenesis in bombyx mori. I. The canal system joining sister spermatocytes
Authors: King Robert C. , Akai Hiromu
Published: February 2005

An electron microscopic investigation was made of the origin and morphology of the canal system that connects sister spermatocytes of larval Bombyx mori. Canal formation results from incomplete cleavages during the mitoses which immediately precede meiosis. We suggest the lip which surrounds each canal is derived from the contractile ring which is prevented from constricting completely by interaction with the midbody. Cells were found with as many as four canals which demonstrates that canal rims can persist through a number of mitotic cycles. Speculations are advanced as to the significance of these canals in restricting gonocytes from unlimited divisions.
Striated visceral muscle of Drosophila melanogaster
Authors: Goldstein Margaret Ann , Burdette Walter J.
Published: February 2005

Striated visceral muscle cells are scattered singly or in small groups at the base of the intestinal cells of the mid‐gut of Drosophila melanogaster larvae. Fibers less than 1 μ in diameter, designated as small, contain a single myofibril, few, if any, dyads and few mitochondria. Fibers of somewhat larger diameter contain dyads and more mitochondria. Both types of fiber have a perforate Z band which appears as discontinuous bodies in longitudinal sections and as a perforate sheet of dense rims and clear perforations in transverse sections. The Z rims contain filaments, 30 to 50 Å, oriented in the transverse direction. The number and arrangement of myofilaments and the ultrastructure of the Z band are consistent with the function of these muscles.
The number of centrioles in insect sperm: A study in two kinds of differentiating silkworm spermatids
Authors: Friedländer M. , Wahrman J.
Published: February 2005

The typical eupyrene and atypical apyrene cycles of sperm differentiation in Bombyx mori were studied, with special attention to centriole number and behavior. Contrary to other reports, there is always only one centriole in the differentiating and in the mature sperm, thus confirming our previous findings that insect sperm has one centriole at the base of the flagellum, in contrast to two centrioles found in many other groups of animals. This numerical difference is discussed in an evolutionary context.
A gross study of the compartmentalized stomach of two new‐world camelids, the llama and guanaco
Authors: Vallenas A. , Cummings J. F. , Munnell J. F.
Published: February 2005

The llama and guanaco stomach consisted of three compartments. A transverse pillar divided the large, first compartment into cranial and caudal sacs. Both sacs contained recessed glandular saccules. The saccules in the caudal sac were everted during the gastric contraction cycle. The non‐recessed surfaces of this compartment were covered by stratified squamous epithelium.

The first compartment communicated on the right with a smaller, reniform second compartment. Except on the lesser curvature, this compartment contained deep cells which were lined by a papillated glandular mucosa.

The ventricular groove, defined by a single muscular lip, coursed along the cranial sac of the first compartment, over the lesser curvature of the second compartment, and terminated at the tubular passage to the third compartment.

The initial four‐fifths of the elongate third compartment contained mucigenous glands like those found in the saccules and cells of the first and second compartment Proper gastric glands and pyloric glands were confined to the terminal one‐fifth of the third compartment.

Attempts to homologize the compartments of the camelid stomach with those of the Pecora or so‐called advanced ruminants were unsuccessful. The results of this study and concurrent physiologic investigations indicated that the processes of ruminant digestion can operate within wide anatomic boundaries, and that the camelid stomach with its extensive glandular mucosa is adapted for greater digestive efficiency than the advanced ruminant stomach.
An electron microscopic study of the corpus cardiacum of adult Drosophila melanogaster and its afferent nerves
Authors: Aggarwal Surinder K. , King Robert C.
Published: February 2005

The left and right afferent nerves to the corpus cardiacum contain approximately 50 and 90 axons, respectively, when they enter the gland. Both bifurcate within the gland, so that four nerves leave the corpus cardiacum. These, however, contain a total of 90 axons. Of the 50 axons unaccounted for 30 are believed to terminate in the corpus cardiacum and 20 in the corpus allatum. The corpus cardiacum contains 12 intrinsic neurosecretory cells which are disposed laterally with respect to the extrinsic axons. Many of these contain neurosecretory spheres and presumably originate in the brain. The intrinsic corpus cardiacum cells also contain neurosecretory granules, and they produce short cytoplasmic processes which interdigitate with the extrinsic axons.
Ultrastructure of the epidermis in the ice worm, Mesenchytraeus solifugus
Authors: Goodman Daniel , Parrish Wayne B.
Published: February 2005

The ice worm is adapted for life at O°C. A survey of the ultrastructure of the cuticle, epidermal epithelium and basement membrane does not reveal any features which self‐evidently correlate with such metabolic specialization; instead, these tissues are much like those of the earthworm and some freshwater oligochaetes. The cuticular fibers are unstriated. Epithelial cells aresuggested as the source of cuticular material. Epithelial microvilli penetrate the cuticle. There is an array of membrane bound bodies on the cuticle surface. The basement membrane fibers are transversely striated and are oriented in crossed lamellae. The junctional complex is represented by azonula adhaerens and septate desmosome.
Reproductive systems of the north indian langur (Presbytis entellus entellus Dufresne)
Authors: David G. F. X. , Ramaswami L. S.
Published: February 2005

The gross anatomy and histology of the gonads and accessory sex organs are described for male and female Presbytis e. entellus. The langur differs from other catarrhine monkeys in certain specialised characters. The “sexual skin” is not comparable to the true sexual skin of the Cercopithecinae which exhibits cyclical change during the various phases of reproduction; it is nevertheless fully developed in the adult male and serves as one of the secondary sexual characters.

Certain aspects of the reproductive system strikingly resemble those of man. The combined testicular weight (0.07% body weight) is similar to the human (0.08%), and the male has ampullary glands. The cervical canal is straight like that of a baboon or man. The corpus luteum (except in the lactating female) is a hollow glandular structure. Extensive hemorrhage which always accompanies ovulation in the langur, does not appear to be a common phenomenon in any other catarrhine for which ovulation and the development of corpus luteum have been studied. The hemorrhagic remains are retained for a long time in the ovulated follicle.

The ovary is characteristically large and averages 0.74 gm without corpus luteum and 1.57 gm with corpus luteum, a feature never reported in any other catarrhine monkeys. The pre‐ovulatory follicle may attain a size of 14 × 14 mm.
The ultrastructure of developing wings in the giant silkmoth, Hyalophora cecropia. II. Scale‐forming and socket‐forming cells
Authors: Greenstein Masha Etkin
Published: February 2005

In wings of the giant silkmoth, Hyalophora cecropia, scale‐forming and socket‐forming cells are first observed on day four of pharate adult development. Scale‐forming cells appear synthetically active when they are first observed, for their basal region is filled with huge stacks of polyribosome‐studed lamellate endoplasmic reticulum, numerous Golgi complexes containing secretory vesicles and many elongated mitochondria. During later development, some of these organelles diminish in number. Neck and scale regions are predominantly filled with longitudinally oriented microtubules and microfibril bundles, suggesting that their function is one of transport rather than synthesis. The scales originate as finger‐like projections of the cell body. They subsequently elongate, flatten out and deposit a cuticle which has a surface differing somewhat from that in other lepidopterans. It consists of longitudinal ridges (1.8‐2.4 μ apart), transverse ribs (0.6‐1.0 μ apart) and microribs (0.10‐0.13 μ apart).

Socket‐forming cells produce a socket around the neck region of each scale‐forming cell. The socket differentiates into several morphologically distinct regions: an apical fibrillar region, a ribosomal region, a mitochondrial‐microtubular region and a basal fibrillar region. The absence of polyribosome‐studded lamellae of endoplasmic reticulum and Golgi complexes suggests that its primary function is not biosynthesis but support and protection of the scale.
Necleolar reorganization in epithelial cells of the jejunum of the rat
Authors: Adamstone F. B. , Taylor A. B.
Published: February 2005

Light microscope studies were made on nucleoli of jejunal epithelial cells of normal rats fixed in OsO4, glutaraldehyde, F.A.A. and HgCl2 and stained with basic fuchsin–alkaline methylene blue. Nucleolar reorganization is extensive and clearly resembles the phenomenon of nucleolar segregation. Polymorphous nucleoli of undifferentiated crypt cells show intermingled constituents and stain purple whereas similar nucleoli of definitive absorptive cells show two homogeneous components–A, stained red and B, stained blue. Cytochemical studies indicate that component A is largely protein and acidophilic and component B is largely nucleic acids and basophilic. These nucleoli become compacted, each forming an amphinucleolus with the two components at opposite poles. Further changes occur along the villi and the components generally separate to form a condensed plasmosome and a diffuse karysome. Extruded cells show nucleolar fragmentation.

Electron micrographs of OsO4 material were used in preparation of wax models. These, along with electron micrographs of glutaraldehyde material stained with uranyl acetate and lead citrate, clearly illustrate and duplicate light microscope findings and strongly resemble nucleolar segregation produced by antimetabolites.

Cells of the villi with reorganized nucleoli do not undergo mitosis whereas undifferentiated crypt cells do so. Furthermore, nucleolar reorganization is correlated with aging since it begins in crypt cells and culminates in senescent cells at the tips of the villi.

A review is given of the extensive evidence showing that, in the intestine certain functional changes occur similar to those demonstrated in experimental nucleolar segregation. These include gradually changing patterns of DNA, RNA and protein synthesis as well as enzyme activity. The accompany and probably result from nucleolar reorganization.
The structure of the ventricle of Elliptio complanatus, a fresh‐water lamellibranch
Authors: Rutherford John G.
Published: February 2005

The morphology of the ventricle of the fresh‐water lamellibranch, Elliptio complanatus, was investigated. Contrary to the condition reported previously in Tritogonia verrucosa, the two atria in Elliptio communicate with the ventricular lumen through separate openings, each guarded by an atrio‐ventricular valve. Fixation of ventricle for electron microscopy with 2.5% buffered glutaraldehyde did not appear to shrink the tissue, in spite of the low blood osmolarity to which the muscle is adapted. Ventricle tissue is composed of smooth muscle fibers, containing a central nucleus, glycogen, mitochondria, paramyosin, dense bodies and “attachment plaques,” much like the ventricle of the salt‐water clam, Venus (Mercenaria) mercenaria.
A cytological study of the ovary of Rhodnius prolixus. I. The ontogeny of the follicular epithelium
Authors: Huebner Erwin , Anderson Everett
Published: February 2005

The relatively undifferentiated cells comprising the prefollicular epithelium of the fourth and fifth instar of the reduvid bug Rhodninus prolixus are flattened and contain the regularly occurring organelles, lipid droplets, and aggregates of glycogen‐like particles. These cells transform into the adult prefollicular tissue.

During vitellogenesis there is a gradual shortening of the cells of the follicular epithelium and an increase in the size of the intercellular space between them and between follicle cells and oocyte. The follicle cells are binucleate, contain numerous microtubules, rough endoplasmic reticulum, many free and aggregate ribosomes, and Golgi complexes. They are associated with each other by gap junctions. Only the follicle cells on the lateral aspects of the oocyte exhibit the development of large extracellular spaces while those at the apical end, that produces the cap, remain tall and closely apposed to each other during vitellogenesis. The normal morphology of the follicle cells over various areas of the oocyte suggests that shape and/or volume changes of these cell may be important in regulating the access of yolk proteins to the colemma.

Subsequent to vitellogenesis the follicle cells become cuboidal and once again become closely apposed to each other. They contain much rough endoplasmic reticulum and produce the secondary coat.
Ultrastructural observations on oogenesis in Drosophila
Authors: Mahowald Anthony P.
Published: February 2005

The ultrastructure of the follicle cells and oocyte periplasm is described during the stages of oogenesis immediately prior to, during, and immediately subsequent to, vitellogenesis. A number of features have not been described previously in Drosophila. Some yolk appears prior to pinocytosis of blood proteins. However, most of the protein yolk forms while the periplasm is filled with micropinocytotic invaginations and tubules derived from the oolemma. These tubules retain the internal layer of material characteristic of coated vesicles and are found to fuse with yolk spheres. No accumulation of electron‐dense material in the endoplasmic reticulum or Golgi of the oocyte is found. Both trypan blue and ferritin are accumulated by the oocyte.

The follicle cells have an elaborate endoplasmic reticulum during the period of maximum yolk accumulation. Adjacent cells are joined at their base by a zonula adhaerens, forming a band around the cells, and by plaques of gap junctions. Gap junctions are also present between nurse cells and follicle cells. During chorion formation, septate junctions also appear between follicle cells, adjacent to the zonula adhaerens.
Formed elements of the blood of the fresh water mussel, Lamellidens corrianus
Authors: Narain Arun Shanker
Published: February 2005

The formed elements of the blood of L. corrianus are comprised exclusively of amoebocytes, whose total number is approximately 758 per cubic mm of blood. The blood cells are of three types, viz. acidophils, large basophils and small basophils, which are respectively 26.32%, 60.20% and 13.48% of the total blood cell population. The cytoplasm of the acidophils is filled with large acidophilic granules, while that of the basophils contains fine basophilic granules. Acidophils are the largest (7.6 μ × 6.7 μ), large basophils are smaller (6.5 μ × 4.9 μ), and small basophils are the smallest (4.1 μ × 3.8 μ). The nucleus occupies a very small volume of the cell (approx. 1/9th) in acidophils, a larger volume (approx. 1/4th) in large basophils, and a major volume (approx. 3/4th) in small basophils. The blood cells show amoeboid movement, which is brought about by means of broad lamellate pseudopodia. Large basophils move very slowly, acidophils move faster, and small basophils move quite fast. Fine bristle‐like filipodia help the blood cells in getting entangled to form clumps. Filipodia are more abundant in large basophils and acidophils which pertake more actively in clumping than small basophils which rarely possess filipodia. Clumping is a reversible process which is promoted by mechanical agitation of blood.
Morphogenesis of intestinal villi. I. Scanning electron microscopy of the duodenal epithelium of the developing chick embryo
Authors: Grey Robert D.
Published: February 2005

Development of villi in the duodenum of the chick was studied in stages ranging from 11 days of incubation to one week after hatching. Formation of definitive villi is preceded by development of a set of previllous ridges that run lengthwise along the duodenum. The first set of 16 previllous ridges (Set I) is complete by about 13 days of incubation; all ridges in the set are fairly uniform and proceed through their subsequent development in synchrony. Previllous ridges in Set I fold into a highly regular zigzag pattern between 14 and 16 days of incubation. Definitive villi develop from Set I ridges beginning at about 17 days when populations of distinct cells appear on the crests of the ridges between angles in the zigzag folds. Cells in these populations lack the rounded appearance of cells seen in earlier stages; their apical surfaces are densely covered with microvilli. A second set of villi (Set II) develops at about 16 days of incubation when about 16 rows of tongue‐like flaps erupt between the previllous ridges of Set I. At hatching, Set II villi are still smaller than villi of Set I; this distinction disappears by about the fourth day after hatching. The significance of the morphological changes in epithelial cells is discussed in terms of several hypotheses bearing on the mechanisms of villus formation.
Origin of mono‐ and binucleate cells with heterochromatic chromosomes in the malpighian tubules of the european elm scale, Gossyparia spuria (Coccoidea: Homoptera)
Authors: Nur Uzi
Published: February 2005

Males of the European elm scale, Gossyparia spuria (Erioccoccidae) have two Malphigian tubules, each made up of mononucleate and binucleate cells. Both types of cells may contain heterochromatic (H) chromosomes which form an H body. The cells with H bodies (H cells) usually appeared singly anywhere along the tubule. However, when two or more H cells were present they tended to be closer to each other than would be expected by chance. The possible origin of this tendency is discussed. Following squashing, the nuclei of the binucleate cells were much larger than those of most other somatic cells, suggesting that they were highly endopolyploid. However, the H bodies of the cells of the tubules were of about the same size as those of the other cells. These observations suggested that the H chromosomes of the binucleate cells did not replicate while the euchromatic chromosomes of these cells replicated several times. The great majority of the nuclei of the H cells contained a single H body per nucleus. An analysis of the number of H bodies in binucleate cells indicated that when two H bodies were present in the same nucleus they usually did not fuse. Thus, they were believed also not to fuse in the mononucleate cells. Since almost all the mononucleate H cells had only a single H body (rather than 2) it was concluded that they did not originate from binucleate cells by nuclear fusion.