bluuebunny/crossref_metadata_2025 huggingface dataset:
Feedforward-plus-proportional–integral–derivative controller for agricultural robot turning in headland
Authors: Huang Peichen , Zhang Zhigang , Luo Xiwen
Published: January 2020

Traveling along straight lines and headland turning are two common motions during the automatic guidance of agricultural machines. However, most studies focus on accurately following parallel tracks in the field and seldom consider the maneuvers at the end of each row. Moreover, numerous studies have mainly focused on planning the global trajectories in the entire field to increase field efficiency, and very few works are related to maneuver generation and vehicle control in headlands. In this study, a feedforward-plus-proportional–integral–derivative controller for a differential drive robot in headland turning was developed. The feedforward-plus-proportional–integral–derivative controller consisted of a feedforward and a feedback loop. The feedforward loop was designed based on the heading errors of a lookahead point on the planning path. The feedback loop was designed based on radial errors to improve the tracking accuracy. Field comparison tests of feedforward, feedback, and feedforward-plus-proportional–integral–derivative controllers were conducted. Experimental results showed that the feedforward-plus-proportional–integral–derivative had better tracking results and took less turning time.
Efficient hindsight reinforcement learning using demonstrations for robotic tasks with sparse rewards
Authors: Zuo Guoyu , Zhao Qishen , Lu Jiahao , Li Jiangeng
Published: January 2020

The goal of reinforcement learning is to enable an agent to learn by using rewards. However, some robotic tasks naturally specify with sparse rewards, and manually shaping reward functions is a difficult project. In this article, we propose a general and model-free approach for reinforcement learning to learn robotic tasks with sparse rewards. First, a variant of Hindsight Experience Replay, Curious and Aggressive Hindsight Experience Replay, is proposed to improve the sample efficiency of reinforcement learning methods and avoid the need for complicated reward engineering. Second, based on Twin Delayed Deep Deterministic policy gradient algorithm, demonstrations are leveraged to overcome the exploration problem and speed up the policy training process. Finally, the action loss is added into the loss function in order to minimize the vibration of output action while maximizing the value of the action. The experiments on simulated robotic tasks are performed with different hyperparameters to verify the effectiveness of our method. Results show that our method can effectively solve the sparse reward problem and obtain a high learning speed.
An Antiproliferative ent-Kaurane Diterpene Isolated from the Roots of Mallotus japonicus Induced Apoptosis in Leukemic Cells
Authors: Lee Joo-Eun , Thanh Thuy Nguyen Thi , Lee Youngju , Cho Namki , Yoo Hee Min
Published: January 2020

Mallotus japonicus has been evaluated for the treatment of dermatitis, inflammatory conditions, and cancer. Diterpenes, one of the major constituents of M. japonicus, possess various pharmacological effects. In this study, 2 known diterpenes, anomaluone (6) and 16-epiabbeokutone (7), along with other known compounds, 2-hydroxy ferulic acid (1), bergenin (2), gallocatechin (3), catechin (4), erythro,erythro-1-[4-[2-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-1-(hydroxymethyl) ethoxy]-3,5-dimethoxyphenyl]-1,2,3- propanetriol (5), and gallincin (8), were isolated from M. japonicus. Cytotoxicity assays in blood cancer cell models demonstrated that M. japonicus compounds possess potent antiproliferative activity. In addition, treatment with compound 6 increased the number of apoptotic cells, led to cell-cycle arrest at the subG0/G1 phase, and decreased the number of cells in the S and G2/M phases. Compound 6 also displayed potent mitochondrial depolarization effects in Jurkat cells. These findings revealed that the cytotoxic effects of 6 were mediated by intracellular signaling, possibly through a mechanism involving upregulation of mitochondrial reactive oxygen species. Thus, compound 6 could be a potential multi-target therapeutic agent for leukemia.
Endoscopic Flexor Hallucis Longus Transfer for Achilles Noninsertional Tendinopathy: Description of Surgical Technique and Functional Outcomes
Authors: Gerstner G Juan Bernardo , Winson Ian , Campo Jimmy , Swords Michael , Camilo Medina Juan , Rammelt Stefan , Gerstner S Jochen , Cantor Erika J , Ramírez Carlos
Published: January 2020

Background. The transfer of the flexor hallucis longus (FHL) tendon is an established treatment for replacing a dysfunctional Achilles tendon. Objectives. (1) Describe a new technique for endoscopic FHL transfer for noninsertional Achilles tendinopathy and (2) describe the functional outcomes and complications after endoscopic and open FHL transfer. Materials and Method. Retrospective study of patients who underwent open or endoscopic FHL transfer between 2014 and 2016. The American Orthopaedic Foot & Ankle Society (AOFAS) ankle/hindfoot scale was used preoperatively and postoperatively to measure the functional results. Results. We included 18 endoscopic FHL transfers and 24 open FHL transfers. The mean age of endoscopic and open procedures was 47.5 years (range 25-77 years) and 61.2 years (range 43-72 years), respectively. An improvement on the average AOFAS of 52.8% (31.9 points) was observed in the endoscopy group during the follow-up from the baseline. The mean improvement in AOFAS score for the open group was 41.4% (24.5 points). Four and 7 cases reported complications in the endoscopy and open FHL transfer groups, respectively. Conclusion. While both procedures were effective in treating noninsertional Achilles tendinopathy, the described arthroscopic treatment led to a greater improvement in the AOFAS score and is slightly less prone to lasting complications.

Level of Evidence: Level IV: Case series
Behavioural cardiovascular risk factors and prevalence of diabetes in subjects with familial hypercholesterolaemia
Authors: Perez-Calahorra Sofia , Civeira Fernando , Guallar-Castillón Pilar , Pinto Xavier , Banegas José R , Pedro-Botet Juan , Suarez-Tembra Manuel , Mauri Marta , Soler Cristina , Rodriguez-Artalejo Fernando , Laclaustra Martín
Published: January 2020

A low prevalence of type 2 diabetes mellitus has been reported in familial hypercholesterolaemia. Whether a healthier lifestyle could explain it has not been explored. This cross-sectional study determines the prevalence of lifestyle-related cardiovascular risk factors in heterozygous familial hypercholesterolaemia (HeFH) from the Dyslipidaemia Registry of the Spanish Atherosclerosis Society and in the ENRICA study, a representative sample of the adult Spanish general population, weighted to match the age and sex distribution of the HeFH sample. A total of 2185 HeFH patients and 11,856 individuals from ENRICA were included. HeFH had lower body mass index and fewer of them were smokers than in the reference population. A model adjusted for age, sex and body mass index showed that HeFH more frequently had cardiovascular disease (odds ratio (OR) 23.98; 95% confidence interval (CI) 18.40–31.23) and hypertension (OR 1.20; 95% CI 1.07–1.35), and took anti-hypertensive medication (OR 1.36; 95% CI 1.18–1.56) and anti-diabetic medication (OR 1.25; 95% CI 1.00–1.56), but less frequently were smokers (OR 0.79; 95% CI 0.71–0.89). In a HeFH subsample ( n = 513) with complete blood glucose information, those patients without cardiovascular disease showed lower prevalence of smoking and type 2 diabetes mellitus, lower body mass index and glucose, and higher diastolic blood pressure than the Spanish population. The differences in type 2 diabetes mellitus were justified mostly by the difference in body mass index. Body mass index adjustment also showed higher prevalence of hypertension and use of anti-hypertensive drugs in HeFH. In summary, HeFH patients had lower body mass index, which may contribute to explaining the lower prevalence of diabetes, and lower current smoking but higher hypertension.
The patterns of Chinese authoritarian patronage and implications for foreign policy: Lessons from Sri Lanka, Myanmar and Cambodia
Authors: Hein Patrick
Published: January 2020

This study compares Chinese autocracy promotion in Sri Lanka, Myanmar and Cambodia from the perspective of mass atrocities. Theory posits that foreign powers influence the thinking and behaviour of domestic elites through external incentives. It is the purpose of the article to identify the incentives that link Chinese foreign policy to repressive outcomes as they unintentionally and indirectly reinforce domestic ethno-nationalist narratives and therefore the likelihood and risk of mass atrocities. What are the implications? The so-called “black knight” is not as powerful as some scholars wish to portray: China has become hostage to its own incentives and this will ultimately threaten to undermine its foreign policy goals as the reorientation of foreign policy in Sri Lanka, opposition against Chinese projects in Myanmar and public discontent of the Cambodian opposition with China have demonstrated.
Using Conflict Management in Improving Owners and Contractors Relationship Quality in the Construction Industry: The Mediation Role of Trust
Authors: Iyiola Kolawole , Rjoub Husam
Published: January 2020

This study investigates conflict management climate as perceived by the owners and contractors significantly affect trust and relationship quality in the Nigerian construction industry. This empirical study also assesses trust as a mediator between conflict management climate and relationship quality. Data gathered from 426 owners and contractors employees in the Nigerian construction industry were used to verify the abovementioned relationships via structural equation modeling. The results show that conflict management climate significantly predicted trust and relationship quality. Trust significantly predicted relationship quality and partially mediates the relationship between conflict management climate and relationship quality. This study contributes to theory within this research by showing trust and relationship quality is significantly affected when owners and contractors are aware of the conflict management climate. The study offers important practical implications for managing conflict management between owners and contractors in the construction industry. Insights into future research directions are also documented.
Knowledge About Treatment, Anxiety, and Depression in Association With Internet-Based Cognitive Behavioral Therapy for Adolescents: Development and Initial Evaluation of a New Test
Authors: Berg Matilda , Andersson Gerhard , Rozental Alexander
Published: January 2020

Evaluating knowledge and learning in psychotherapy is a growing field of research. Studies that develop and evaluate valid tests are lacking, however. Here, in the context of internet-based cognitive behavioral therapy (ICBT) for adolescents, a new test was developed using subject matter experts, consensus among researchers, self-reports by youths, and a literature review. An explorative factor analysis was performed on 93 adolescents between 15 and 19 years old, resulting in a three-factor solution with 20 items, accounting for 41% of the total variance. The factors were Act in aversive states, Using positive reinforcement, and Shifting attention. The internal consistency for the whole instrument was good (α = .84). Hopefully, the procedure presented here can contribute to the field by illustrating one way of evaluating knowledge in ICBT. The clinical use of the knowledge test needs further investigation.
Revisionist Conflict and State Repression
Authors: Wright Thorin M.
Published: January 2020

What kinds of international conflicts make states more likely to increase repression? I argue that the issues at stake in conflict may have different levels of domestic salience and may alter the domestic political status quo, thus increasing or decreasing a state’s or regime’s propensity to repress. I argue and find that democracies are most likely to increase repression when they are territorial revisionists, specifically increasing the use of imprisonment and torture. Autocratic states are more likely to increase repression during foreign policy-oriented disputes, as opposed to those fought over territory, which are less likely to escalate to full-scale war, and more likely to be domestically motivated. This project thus opens up the black box of international conflict to better understand how the reasons states fight abroad affects decisions to repress at home.
Ethno-political favouritism in maternal health care service delivery: Micro-level evidence from sub-Saharan Africa, 1981–2014
Authors: Theisen Ole Magnus , Strand Håvard , Østby Gudrun
Published: January 2020

It is commonly held that political leaders favour people of the same ethnic origin. We test this argument of ethno-political favouritism by studying variations in the usage of maternal health care services across groups in sub-Saharan Africa (SSA). More specifically, we link geo-referenced individual-level data from the Demographic and Health Surveys on 601,311 births by 399,908 mothers in 31 countries during the period 1981–2014 with data on the settlement of ethnic groups and their political status. Our results indicate that women benefit from the shift that brings co-ethnics into power, increasing the probability of receiving maternal health care services. The effect strengthens with increased competitiveness around elections. We advance the current literature in four important ways. Firstly, we undertake the first analysis that utilizes shifts in ethno-political status for the same individual, effectively eliminating competing time-invariant explanations to that of shifts in ethno-political status. Secondly, since SSA governments often incorporate multiple groups, we test the effect of patronage on being co-ethnic with cabinet members in general, and not only the president. Thirdly, health services constitute the public good most desired by citizens of SSA. Our measure captures a vital health service that is highly desired across groups. An increase in usage likely reflects genuine trickle-down effects of having co-ethnics in power, a crucial ingredient in building popular support for ethnic patrons. Fourthly, we show that electoral competition is an important conditioner of ethno-political favouritism.
Clinical outcomes of meniscus repair and partial meniscectomy: Does tear configuration matter?
Authors: Gan Jonathan Zhi-Wei , Lie Denny TT , Lee Wen Qiang
Published: January 2020

Purpose:

We report our experience with the effect of location and configuration of meniscal tears on clinical outcomes.

Methods:

A retrospective review of patients who underwent partial meniscectomy or meniscal repair between 2008 and 2016 was conducted. One hundred fourteen knees in 106 patients were included, comprising 43 partial meniscectomies and 71 meniscal repairs. Patients were graded pre- and postoperatively with the International Knee Documentation Committee (IKDC) score and Tegner Activity Level Scale. Meniscal tears were classified according to location (anterior horn, body, posterior horn, and others) and type (radial, horizontal, longitudinal, and complex) and subgroups were analyzed for their effect on outcomes.

Results:

All tears, whether treated with partial meniscectomy or repair, showed significant improvement in postoperative scores ( p < 0.05); 39.47% of tears involved the posterior horn alone, 10.53% involved the body alone, 3.51% involved the anterior horn alone, and 46.49% were complex tears that spanned more than one area. Complex tears treated with repair had significantly better scores (IKDC, p = 0.002; Tegner, p = 0.008) than complex tears treated with meniscectomy. Longitudinal tears showed results suggesting better short-term outcomes with meniscectomy than with repair (IKDC, p = 0.036; Tegner p = 0.018), a potential statistical anomaly. Horizontal and radial tears showed no significant difference in outcomes, regardless of treatment. Tears in different locations (anterior horn, body, posterior horn, and others) showed no significant difference in outcomes, regardless of treatment.

Conclusion:

Meniscal surgery yields significant improvement in postoperative scores. Complex tears showed significantly better postoperative scores when treated with repair.
Shoulder arthroplasty for juvenile idiopathic arthritis
Authors: Madanipour Suroosh , Prinja Aditya , Lee Marcus , Rashid Abbas
Published: January 2020

There is limited literature to guide shoulder surgeons in the management of juvenile idiopathic arthritis (JIA). We aim to help clinicians to formulate an approach to the surgical management of the condition through a review of the available literature on arthroplasty in JIA, general considerations when operating on patients with inflammatory arthropathy and recommendations based on the authors’ experience. Four articles report formal data on arthroplasty in JIA with favourable improvements in post-operative pain and function scores after the long-term follow-up. Significant heterogeneity in treatment and a lack of standardisation in quantitative outcomes highlights the need for further larger scale and higher quality research. The aim of this study is to review the evidence and provide information on preoperative evaluation of surgical candidates, operative techniques, choice of implant design and to evaluate functional outcomes in patients who undergo shoulder arthroplasty.
Differences in patient-reported outcomes between medial opening-wedge high tibial osteotomy and total knee arthroplasty
Authors: Matsumoto Kazu , Ogawa Hiroyasu , Yoshioka Hiroki , Akiyama Haruhiko
Published: January 2020

Purpose:

To compare patient subjective satisfaction between medial opening-wedge high tibial osteotomy (HTO) and total knee arthroplasty (TKA).

Methods:

This study enrolled 110 knees, including comprising 49 knees in the HTO group, and 61 knees in the TKA group. We assessed the overall satisfaction using a three-point questionnaire. The satisfaction questionnaire included three questions: (1) How satisfied are you with the results of your knee surgery? (2) How satisfied are you with your most recent knee surgery for reducing your pain? and (3) How satisfied are you with your most recent knee surgery for improving your ability to perform functions? Furthermore, we assessed knee pain and function by using the Knee Society Function Score (KSS) and Knee Injury and Osteoarthritis Outcome Score (KOOS) systems.

Results:

Overall, 93.8% of patients from the HTO group and 95.1% from the TKA group indicated subjective satisfaction (very satisfied and satisfied) with their surgeries. For pain relief, the HTO group showed significantly better outcomes for overall satisfaction ( p = 0.04 in walking on a flat surface and p = 0.02 in going upstairs or downstairs). For restored function, the HTO group scored significantly better on ascending stairs than the TKA group ( p = 0.007). Functional outcomes using the KSS scoring system did not show significant differences between the two groups. The KOOS pain score was significantly higher in the TKA group (89.9 ± 6.4) than in the HTO group (80.3 ± 12.5).

Conclusion:

HTO and TKA have comparable outcomes with respect to overall patient satisfaction.

Level of evidence:

Level III, therapeutic case series.
The 2018 Municipal Elections in Jerusalem: A Tale of Fragmentation and Polarization
Authors: Nikolenyi Csaba
Published: January 2020

This article analyzes the 2018 local elections in Jerusalem, the contested capital of the State of Israel. These elections were unique in terms of their level of competitiveness and fragmentation as well as producing a highly divided local government in the wake of the incumbent mayor’s, Nir Barkat’s, decision to leave the local political scene and enter national politics. While his party has no representation in city council, the new mayor of Jerusalem, Moshe Lion, built a broadly based new coalition that includes all parties in the council except for Hitorerut, the party that won the most seats and whose mayoral candidate, Ofer Berkovitch, was the runner-up to Lion. With the exception of the ultra-orthodox parties, national political parties that sought to interfere with the local electoral process to promote their candidates and lists by and large failed. Therefore, the governance of the city of Jerusalem once again fell under the control of the ultra-orthodox majority. Furthermore, even though the Arab population of East Jerusalem largely continued its traditional abstention from the electoral process, there was some evidence to suggest that a slight shift was taking place in that community in favor of participating in the institutional process of municipal government and democracy.
Is the Project Manager’s Road to Success Paved Only with Clear Career Paths?
Authors: Lo Presti Alessandro , Elia Angelo
Published: January 2020

This study examined the role of career attitudes and behaviors and employability enhancement factors in predicting project managers’ career success facets, over and above the availability of clear career paths. An online survey was completed by 552 Italian project managers. Clear career paths, employability culture, and protean career attitude positively predicted both objective and subjective (along with objective boundarylessness) career success. Dominance analysis showed the prevailing role of protean career, employability culture, and clear career paths. Results built a bridge between project management literature and career studies, particularly informing about the significance of the protean career. Organizational and individual implications for practices are discussed.
Novel antithrombotic strategies for treatment of venous thromboembolism
Authors: Weitz Jeffrey I. , Chan Noel C.
Published: January 2020

Venous thromboembolism (VTE), which includes deep vein thrombosis (DVT) and pulmonary embolism (PE), is the third most common cause of vascular death after heart attack and stroke. Anticoagulation therapy is the cornerstone of VTE treatment. Despite such therapy, up to 50% of patients with DVT develop postthrombotic syndrome, and up to 4% of patients with PE develop chronic thromboembolic pulmonary hypertension. Therefore, better therapies are needed. Although direct oral anticoagulants are more convenient and safer than warfarin for VTE treatment, bleeding remains the major side effect, particularly in cancer patients. Factor XII and factor XI have emerged as targets for new anticoagulants that may be safer. To reduce the complications of VTE, attenuation of thrombin activatable fibrinolysis inhibitor activity is under investigation in PE patients to enhance endogenous fibrinolysis, whereas blockade of leukocyte interaction with the vessel wall is being studied to reduce the inflammation that contributes to postthrombotic syndrome in DVT patients. Focusing on these novel antithrombotic strategies, this article explains why safer anticoagulants are needed, provides the rationale for factor XII and XI as targets for such agents, reviews the data on the factor XII– and factor XI–directed anticoagulants under development, describes novel therapies to enhance fibrinolysis and decrease inflammation in PE and DVT patients, respectively, and offers insights into the opportunities for these novel VTE therapies.
Transcriptome analysis of the hippocampus in environmental noise-exposed SAMP8 mice reveals regulatory pathways associated with Alzheimer’s disease neuropathology
Authors: Su Donghong , Li Wenlong , Chi Huimin , Yang Honglian , She Xiaojun , Wang Kun , Gao Xiujie , Ma Kefeng , Zhang Ming , Cui Bo
Published: January 2020

Background

Chronic noise exposure is one environmental hazard that is associated with genetic susceptibility factors that increase Alzheimer’s disease (AD) pathogenesis. However, the comprehensive understanding of the link between chronic noise stress and AD is limited. Herein, we investigated the effects of chronic noise exposure on AD-like changes in senescence-accelerated mouse prone 8 (SAMP8).

Methods

A total of 30 male SAMP8 mice were randomly divided into the noise-exposed group, the control group, and aging group (positive controls), and mice in the exposure group were exposed to 98 dB SPL white noise for 30 consecutive days. Transcriptome analysis and AD-like neuropathology of hippocampus were examined by RNA sequencing and immunoblotting. Enzyme-linked immunosorbent assay and real-time PCR were used to further determine the differential gene expression and explore the underlying mechanisms of chronic noise exposure in relation to AD at the genome level.

Results

Chronic noise exposure led to amyloid beta accumulation and increased the hyperphosphorylation of tau at the Ser202 and Ser404 sites in young SAMP8 mice; similar observations were noted in aging SAMP8 mice. We identified 21 protein-coding transcripts that were differentially expressed: 6 were downregulated and 15 were upregulated after chronic noise exposure; 8 genes were related to AD. qPCR results indicated that the expression of Arc, Egr1, Egr2, Fos, Nauk1, and Per2 were significantly high in the noise exposure group. These outcomes mirrored the results of the RNA sequencing data.

Conclusions

These findings further revealed that chronic noise exposure exacerbated aging-like impairment in the hippocampus of the SAMP8 mice and that the protein-coding transcripts discovered in the study may be key candidate regulators involved in environment-gene interactions.
Spontaneous embryo resorption in the mouse is triggered by embryonic apoptosis followed by rapid removal via maternal sterile purulent inflammation
Authors: Drews Barbara , Landaverde Luis Flores , Kühl Anja , Drews Ulrich
Published: January 2020

Background

In normal mammalian development a high percentage of implantations is lost by spontaneous resorption. This is a major problem in assisted reproduction and blastocyst transfer. Which embryo will be resorbed is unpredictable. Resorption is very fast, so that with conventional methods only final haemorrhagic stages are encountered.

Here we describe the histology and immunohistochemistry of 23 spontaneous embryo resorptions between days 7 and 13 of murine development, which were identified by high-resolution ultrasound (US) in a previous study.

Results

In the early resorptions detected at day 7, the embryo proper was replaced by maternal haemorrhage and a suppurate focus of maternal neutrophils. In the decidua maternal macrophages transformed to foam cells and formed a second focus of tissue dissolution.

In the late resorptions detected at day 9, the embryo underwent apoptosis without involvement of maternal cells. The apoptotic embryonic cells expressed caspase 3 and embryonic blood cells developed a macrophage like phenotype. Subsequently, the wall of the embryonic vesicle ruptured and the apoptotic embryo was aborted into the uterine lumen. Abortion was initiated by degeneration of the embryonic lacunar trophoblast and dissolution of the maternal decidua capsularis via sterile inflammation and accompanied by maternal haemorrhage, invasion of the apoptotic embryo by maternal neutrophils, and contraction rings of the uterine muscle layers.

Conclusions

We conclude that spontaneous resorption starts with endogenous apoptosis of the embryo without maternal contribution. After break down of the foetal-maternal border, the apoptotic embryo is invaded by maternal neutrophils, aborted into the uterine lumen, and rapidly resorbed. We assume that the innate maternal unspecific inflammation is elicited by disintegrating apoptotic embryonic cells.

Graphical abstract
Correction to: Transcriptome profiling of resistance response to Meloidogyne chitwoodi introgressed from wild species Solanum bulbocastanum into cultivated potato
Authors: Bali Sapinder , Vining Kelly , Gleason Cynthia , Majtahedi Hassan , Brown Charles R. , Sathuvalli Vidyasagar
Published: January 2020

Following the publication of this article [1], the authors noted an error in Figure 11.
A crustacean annotated transcriptome (CAT) database
Authors: Nong Wenyan , Chai Zacary Y. H. , Jiang Xiaosen , Qin Jing , Ma Ka Yan , Chan King Ming , Chan Ting Fung , Chow Billy K. C. , Kwan Hoi Shan , Wong Chris K. C. , Qiu Jian-Wen , Hui Jerome H. L. , Chu Ka Hou
Published: January 2020

Background

Decapods are an order of crustaceans which includes shrimps, crabs, lobsters and crayfish. They occur worldwide and are of great scientific interest as well as being of ecological and economic importance in fisheries and aquaculture. However, our knowledge of their biology mainly comes from the group which is most closely related to crustaceans – insects. Here we produce a de novo transcriptome database, crustacean annotated transcriptome (CAT) database, spanning multiple tissues and the life stages of seven crustaceans.

Description

A total of 71 transcriptome assemblies from six decapod species and a stomatopod species, including the coral shrimp Stenopus hispidus, the cherry shrimp Neocaridina davidi, the redclaw crayfish Cherax quadricarinatus, the spiny lobster Panulirus ornatus, the red king crab Paralithodes camtschaticus, the coconut crab Birgus latro, and the zebra mantis shrimp Lysiosquillina maculata, were generated. Differential gene expression analyses within species were generated as a reference and included in a graphical user interface database at http://cat.sls.cuhk.edu.hk/. Users can carry out gene name searches and also access gene sequences based on a sequence query using the BLAST search function.

Conclusions

The data generated and deposited in this database offers a valuable resource for the further study of these crustaceans, as well as being of use in aquaculture development.
Cytological evidence of BSD2 functioning in both chloroplast division and dimorphic chloroplast formation in maize leaves
Authors: Li Heying , Bai Mei , Jiang Xingshan , Shen Rongxin , Wang Huina , Wang Haiyang , Wu Hong
Published: January 2020

Background

Maize bsd2 (bundle sheath defective2) is a classical C4 mutant with defective C4 photosynthesis, accompanied with reduced accumulation of Rubisco (ribulose bisphosphate carboxylase oxygenase) and aberrant mature chloroplast morphology in the bundle sheath (BS) cells. However, as a hypothetical chloroplast chaperone, the effects of BSD2 on C4 chloroplast development have not been fully examined yet, which precludes a full appreciation of BSD2 function in C4 photosynthesis. The aims of our study are to find out the role ofBSD2 in regulating chloroplasts development in maize leaves, and to add new insights into our understanding of C4 biology.

Results

We found that at the chloroplast maturation stage, the thylakoid membranes of chloroplasts in the BS and mesophyll (M) cells became significantly looser, and the granaof chloroplasts in the M cells became thinner stacking in the bsd2 mutant when compared with the wildtype plant. Moreover, at the early chloroplast development stage, the number of dividing chloroplasts and the chloroplast division rate are both reduced in the bsd2 mutant, compared with wild type. Quantitative reverse transcriptase-PCR analysis revealed that the expression of both thylakoid formation-related genesand chloroplast division-related genes is significantly reduced in the bsd2 mutants. Further, we showed that BSD2 interacts physically with the large submit of Rubisco (LS) in Bimolecular Fluorescence Complementation assay.

Conclusions

Our combined results suggest that BSD2 plays an essential role in regulating the division and differentiation of the dimorphic BS and M chloroplasts, and that it acts at a post-transcriptional level to regulate LS stability or assembly of Rubisco.
A prospective, randomized comparison of ultrasonographic visualization of proximal intercostal block vs paravertebral block
Authors: Vlassakov Kamen , Vafai Avery , Ende David , Patton Megan E. , Kapoor Sonia , Chowdhury Atif , Macias Alvaro , Zeballos Jose , Janfaza David R. , Pentakota Sujatha , Schreiber Kristin L.
Published: January 2020

Background

Thoracic paravertebral blockade is an accepted anesthetic and analgesic technique for breast surgery. However, real-time ultrasound visualization of landmarks in the paravertebral space remains challenging. We aimed to compare ultrasound-image quality, performance times, and clinical outcomes between the traditional parasagittal ultrasound-guided paravertebral block and a modified approach, the ultrasound-guided proximal intercostal block.

Methods

Women with breast cancer undergoing mastectomy (n = 20) were randomized to receive either paravertebral (n = 26) or proximal intercostal blocks (n = 32) under ultrasound-guidance with 2.5 mg/kg ropivacaine prior to surgery. Block ultrasound images before and after needle placement, and anesthetic injection videoclips were saved, and these images and vidoes independently rated by separate novice and expert reviewers for quality of visualization of bony elements, pleura, relevant ligament/membrane, needle, and injectate spread. Block performance times, postoperative pain scores, and opioid consumption were also recorded.

Results

Composite visualization scores were superior for proximal intercostal compared to paravertebral nerve block, as rated by both expert (p = 0.008) and novice (p = 0.01) reviewers. Notably, both expert and novice rated pleural visualization superior for proximal intercostal nerve block, and expert additionally rated bony landmark and injectate spread visualization as superior for proximal intercostal block. Block performance times, needle depth, opioid consumption and postoperative pain scores were similar between groups.

Conclusions

Proximal intercostal block yielded superior visualization of key anatomical landmarks, possibly offering technical advantages over traditional paravertebral nerve block.

Trial registration

ClinicalTrials.gov, NCT02911168. Registred on the 22nd of September 2016.
Trimethylamine-N-oxide has prognostic value in coronary heart disease: a meta-analysis and dose-response analysis
Authors: Yao Miao-En , Liao Peng-Da , Zhao Xu-Jie , Wang Lei
Published: January 2020

Background

Previous clinical studies have suggested that trimethylamine-N-oxide (TMAO) could contribute to the development of atherosclerosis cardiovascular disease. However, the synthetic analysis in coronary heart disease (CHD) was not yet performed. We aimed to clarify the relationship between elevated plasma concentrations of TMAO and the incidence of major adverse cardiovascular events (MACE) in CHD patients.

Methods

Meta-analysis and dose-response analysis of hazard ratio data from prospective observational studies reporting on the association between TMAO plasma concentrations and the incidence of MACE in patients with CHD were conducted.

Results

Of the 2369 published articles identified in the search, seven papers, with data from nine cohort studies (10,301 patients), were included in the meta-analysis. Combined data showed that elevated plasma TMAO concentrations could increase 58% higher risk of MACE in patients with CHD (hazard ratios [HR]: 1.58; 95% confidence interval [CI] = 1.35–1.84, P = 0.000). For follow-up ≥ 1 year, it was associated with 62% higher risk of MACE in patients with longer-term than shorter-term (HR for follow-up ≥ 4 years: 1.96; 95% CI = 1.52–2.52 vs one to 3 years: 1.34; 95% CI = 1.26–1.43, P = 0.004). The dose-response analysis revealed a ‘J’ shaped association between TMAO concentration and the incidence of MACE (P = 0.033), with the concentration above 5.1 μmol/L being associated with HR of > 1.

Conclusions

Elevated levels of TMAO are associated with an increased incidence of MACE in patients with CHD. TMAO concentration of 5.1 μmol/L may be a cut-off value for prognosis.
The impact of Centre’s heart transplant status and volume on in-hospital outcomes following extracorporeal membrane oxygenation for refractory post-cardiotomy cardiogenic shock: a meta-analysis
Authors: Kowalewski Mariusz , Raffa Giuseppe Maria , Zieliński Kamil , Alanazi Musab , Gilbers Martijn , Heuts Sam , Natour Ehsan , Bidar Elham , Schreurs Rick , Delnoij Thijs , Driessen Rob , Sels Jan-Willem , van de Poll Marcel , Roekaerts Paul , Meani Paolo , Maessen Jos , Suwalski Piotr , Lorusso Roberto
Published: January 2020

Background

Postcardiotomy cardiogenic shock (PCS) that is refractory to inotropic support remains a major concern in cardiac surgery and is almost universally fatal unless treated with mechanical support. While reported mortality rates on ECMO vary from center to center, aim of the current report is assess if the outcomes differ between centres according to volume and heart transplantation status.

Methods

A systematic search was performed according to PRISMA statement using PubMed/Medline databases between 2010 and 2018. Relevant articles were scrutinized and included in the meta-analysis only if reporting in-hospital/30-day mortality and heart transplantation status of the centre. Paediatric and congenital heart surgery-related studies along with those conducted in the setting of veno-venous ECMO for respiratory distress syndrome were excluded. Differences were assessed by means of subgroup meta-analysis and meta-regression.

Results

Fifty-four studies enrolling N = 4421 ECMO patients were included. Of those, 6 series were performed in non-HTx centres (204 pts.;4.6%). Overall 30-day survival (95% Confidence Intervals) was 35.3% (32.5–38.2%) and did not statistically differ between non-HTx: 33.3% (26.8–40.4%) and HTx centres: 35.7% (32.7–38.8%); Pinteraction = 0.531. There was no impact of centre volume on survival as well: ßcoef = 0.0006; P = 0.833. No statistical differences were seen between HTx and non-HTx with respect to ECMO duration, limb complications, reoperations for bleeding, kidney injury and sepsis. There were however significantly less neurological complications in the HTx as compared to non-HTx centres: 11.9% vs 19.5% respectively; P = 0.009; an inverse relationship was seen for neurologic complications in centres performing more ECMOs annually ßcoef = − 0.0066; P = 0.031. Weaning rates and bridging to HTx and/or VADs were higher in HTx facilities.

Conclusions

There was no apparent difference in survival after ECMO implantation for refractory PCS according to centre’s ECMO volume and transplantation status. Potentially different risk profiles of patients in these centres must be taken account for before definite conclusions are drawn.
Do Bacteremic patients with end-stage renal disease have a fever when presenting to the emergency department? A paired, retrospective cohort study
Authors: Weatherall Sarah L. , Chambers Alison B. , Mermel Leonard A.
Published: January 2020

Background

Fever is a common symptom when patients present to Emergency Departments. It is unclear if the febrile response of bacteremic hemodialysis-dependent patients differs from bacteremic patients not receiving hemodialysis. The objective of this study was to compare Emergency Departments triage temperatures of patients with and without hemodialysis-dependent end-stage rental disease who have Staphylococcus aureus bacteremia and determine the incidence of afebrile S. aureus bacteremia.

Methods

Paired, retrospective cohort study of 37 patients with and 37 patients without hemodialysis hospitalized with Methicillin-resistant or Methicillin-susceptible S. aureus bacteremia. Emergency Department triage temperatures were reviewed for all patients, as were potential confounding variables.

Results

54% (95% CI, 38–70%) and 82% (95% CI 65–91%) of hemodialysis and non-hemodialysis patients did not have a detectable fever (<100.4 °F) at triage. Triage temperatures were 100.5 °F (95% CI 99.9–101.2 °F) and 99.0 °F (95% CI 98.4–99.6 °F) in the hemodialysis and non-hemodialysis cohorts, respectively (p < 0.001). Triage temperature in patients with and without diabetes mellitus was 99.2 °F (95% CI 98.4–99.9 °F) and 100.4 °F (95% CI 99.7–101.0 °F), respectively (p = 0.03). We were unable to detect a significant effect of diabetes mellitus and other potential confounding variables on differences in temperature between the hemodialysis and non-hemodialysis cohorts (all interactions p > 0.19).

Conclusions

Hemodialysis-dependent patients with S. aureus bacteremia had significantly higher temperatures than non- hemodialysis-dependent end stage renal disease patients but more than half of patients were without detectable fever at triage, possibly reflecting use of insensitive methods for measuring temperature. Absence of fever at presentation to the Emergency Department should not delay blood culture acquisition in patients who are at increased risk of S. aureus bacteremia.
Cerebro-spinal fluid biomarker levels: phosphorylated tau (T) and total tau (N) as markers for rate of progression in Alzheimer’s disease
Authors: Wattmo Carina , Blennow Kaj , Hansson Oskar
Published: January 2020

Background

We investigated the potential associations between cerebro-spinal fluid (CSF) levels of phosphorylated tau (P-tau) and total tau (T-tau) with short-term response to cholinesterase inhibitor (ChEI) treatment, longitudinal outcome and progression rates in Alzheimer’s disease (AD).

Methods

This prospective, observational study included 129 participants clinically diagnosed with mild-to-moderate AD, who underwent a lumbar puncture. The CSF biomarkers amyloid-β1–42 (Aβ42), P-tau and T-tau were analysed with xMAP technology. Cognitive, global, instrumental and basic activities of daily living (ADL) capacities at the start of ChEI therapy and semi-annually over 3 years were evaluated.

Results

All patients had abnormal Aβ42 (A+). Fifty-eight individuals (45%) exhibited normal P-tau and T-tau (A+ T– (N)–), 12 (9%) abnormal P-tau/normal T-tau (A+ T+ (N)–), 17 (13%) normal P-tau/abnormal T-tau (A+ T– (N)+) and 42 (33%) abnormal P-tau and T-tau (A+ T+ (N)+). The participants with A+ T+ (N)+ were younger than A+ T– (N)+ at the estimated onset of AD and the initiation of ChEIs. The proportion of 6-month responders to ChEI and deterioration/year after start of treatment did not differ between the AT(N) profiles in any scales. A higher percentage of globally improved/unchanged patients was exhibited in the A+ T– (N)– group after 12, 30 and 36 months of ChEI therapy but not at other assessments. In apolipoprotein E (APOE) ε4-carriers, linear relationships were found between greater cognitive decline/year and higher tau; Mini-Mental State Examination score – T-tau (rs = − 0.257, p = 0.014) and Alzheimer’s Disease Assessment Scale–cognitive subscale – P-tau (rs = − 0.242, p = 0.022). A correlation between faster progression in instrumental ADL (IADL) and higher T-tau was also detected (rs = − 0.232, p = 0.028). These associations were not demonstrated in non-ε4-carriers.

Conclusions

Younger age and faster global deterioration were observed in AD patients with pathologic tau and neurodegeneration, whereas more rapid cognitive and IADL decline were related to higher P-tau or T-tau in APOE ε4-carriers only. The results might indicate an association between more pronounced tau pathology/neuronal injury and the APOE ε4-allele leading to a worse prognosis. Our findings showed that the AT(N) biomarker profiles have limited utility to predict AD progression rates and, thus, measure change and interpreting outcomes from clinical trials of future therapies.
Barriers to provision of respectful maternity care in Zambia: results from a qualitative study through the lens of behavioral science
Authors: Smith Jana , Banay Rachel , Zimmerman Emily , Caetano Vivien , Musheke Maurice , Kamanga Ameck
Published: January 2020

Background

Recently, a growing body of literature has established that disrespect and abuse during delivery is prevalent around the world. This complex issue has not been well studied through the lens of behavioral science, which could shed light on the psychological dimensions of health worker behavior and how their micro-level context may be triggering abuse. Our research focuses on the behavioral drivers of disrespect and abuse in Zambia to develop solutions with health workers and women that improve the experience of care during delivery.

Methods

A qualitative study based on the behavioral design methodology was conducted in Chipata District, Eastern Province. Study participants included postpartum women, providers (staff who attend deliveries), supervisors and mentors, health volunteers, and birth companions. Observations were conducted of client-provider interactions on labor wards at two urban health centers and a district hospital. In-depth interviews were audio recorded and English interpretation from these recordings was transcribed verbatim. Data was analyzed using thematic analysis and findings were synthesized following the behavioral design methodology.

Results

Five key behavioral barriers were identified: 1) providers do not consider the decision to provide respectful care because they believe they are doing what they are expected to do, 2) providers do not consider the decision to provide respectful care explicitly since abuse and violence are normalized and therefore the default, 3) providers may decide that the costs of providing respectful care outweigh the gains, 4) providers believe they do not need to provide respectful care, and 5) providers may change their mind about the quality of care they will provide when they believe that disrespectful care will assist their clinical objectives. We identified features of providers’ context – the environment in which they live and work, and their past experiences – which contribute to each barrier, including supervisory systems, visual cues, social constructs, clinical processes, and other features.

Conclusions

Client experience of disrespectful care during labor and delivery in Chipata, Zambia is prevalent. Providers experience several behavioral barriers to providing respectful maternity care. Each of these barriers is triggered by one or more addressable features in a provider’s environment. By applying the behavioral design methodology to the challenge of respectful maternity care, we have identified specific and concrete contextual cues that targeted solutions could address in order to facilitate respectful maternity care.
Functional versus functional and anatomical criteria-guided ranibizumab treatment in patients with neovascular age-related macular degeneration – results from the randomized, phase IIIb OCTAVE study
Authors: Staurenghi Giovanni , Garweg Justus G. , Gerendas Bianca S. , Macfadden Wayne , Gekkiev Boris , Margaron Philippe , Dunger-Baldauf Cornelia , Kolar Petr
Published: January 2020

Background

To evaluate the efficacy and safety of two individualized ranibizumab retreatment schemes in neovascular age-related macular degeneration.

Methods

Patients (N = 671) were randomized (1:1) to receive three initial monthly ranibizumab 0.5 mg injections, then retreatment guided by either best-corrected visual acuity (BCVA) loss (Group I) or BCVA loss and/or signs of disease activity on optical coherence tomography (OCT; Group II). The study was terminated prematurely and the decision to discontinue the study was made by the sponsor. Efficacy analyses were performed on patients who completed 12 months of the originally planned 24-month study. Safety analyses are presented for all safety analyzable patients.

Results

Of 671 randomized patients, 305 completed 12 months of the study. For the 12-month completers, baseline mean (standard deviation) BCVA and reading-center evaluated central subfield thickness (CSFT) were comparable [Group I: 60.9 (13.10) letters and 517.7(201.79) μm; Group II: 60.2 (12.21) letters and 515.3 (198.37) μm]. The change from baseline at Month 12 in BCVA was 6.7 (13.48) letters in Group I and 8.3 (13.53) letters in Group II and the change in CSFT was − 161.3 (163.48) μm and − 175.3 (170.45) μm, respectively. The mean number of ranibizumab injections was 8.2 in Group I and 8.4 in Group II.

Conclusion

Ranibizumab treatment resulted in visual and anatomic gains at 12 months for both retreatment strategies, with a trend in favor of OCT-guided vs BCVA loss guided retreatment. No new safety signals were seen.

Trial registration

www.ClinicalTrials.gov (NCT01780935). Registered 31 January 2013.