bluuebunny/crossref_metadata_2025 huggingface dataset:
A Scoping Review for Usage of Telerehabilitation among Older Adults with Mild Cognitive Impairment or Cognitive Frailty
Authors: Md Fadzil Nurul Hidayah , Shahar Suzana , Rajikan Roslee , Singh Devinder Kaur Ajit , Mat Ludin Arimi Fitri , Subramaniam Ponnusamy , Ibrahim Norhayati , Vanoh Divya , Mohamad Ali Nazlena
Published: March 2022

Older adults are vulnerable towards cognitive frailty that can lead to adverse health outcomes and telerehabilitation appears to be a potential platform to reverse cognitive frailty among older adults. The aim of this coping review is to identify the usage of telerehabilitation and its common platform of delivery among older adults with mild cognitive impairment (MCI) or cognitive frailty (CF). Articles published from January 2015 until October 2020 were selected. Out of the 1738 articles retrieved, six studies were identified. Two articles were randomized controlled trials, one was a pilot study and three were qualitative studies. The outcome suggests that telerehabilitation may improve the quality of life among participants as well as it can be a useful and supportive digital platform for health care. Some types of technologies commonly used were smartphones or telephones with internet, television-based assistive integrated technology, mobile application and videoconference. Telerehabilitation utilization in managing cognitive frailty among older adults is still limited and more research is required to evaluate its feasibility and acceptability. Although telerehabilitation appears to be implemented among older adults with MCI and CF, some social support is still required to improve the adherence and effectiveness of telerehabilitation. Future research should focus on the evaluation of acceptance and participants’ existing knowledge towards telerehabilitation to achieve its target.
Peripheral Iron Levels in Autism Spectrum Disorders vs. Other Neurodevelopmental Disorders: Preliminary Data
Authors: De Giacomo Andrea , Medicamento Silvia , Pedaci Chiara , Giambersio Donatella , Giannico Orazio Valerio , Petruzzelli Maria Giuseppina , Simone Marta , Corsalini Massimo , Marzulli Lucia , Matera Emilia
Published: March 2022

Autism Spectrum Disorder (ASD) is characterized by deficits in social skills and specific behaviors and interests. Among other environmental factors, iron may play a role in the development of ASD. The aim of this study is to compare the iron status of children with ASD with that of children affected by neurodevelopmental disorders other than ASD (OND). A total of 167 patients were enrolled, including 93 children with ASD and 74 children with OND. In the two groups, we determined ferritin, iron, transferrin, hemoglobin, HCT, and MCV in the serum. We found a significant difference in serum ferritin and MCV levels between the two groups (p < 0.05), with lower ferritin and higher MCV values in the ASD group. There was no significant association with the other variables. Our results may support the hypothesis of altered iron status in ASD, justifying more frequent examinations of blood iron parameters in these children.
Mental Health Symptoms among General Practitioners Facing the Acute Phase of the COVID-19 Pandemic: Detecting Different Reaction Groups
Authors: Carmassi Claudia , Dell’Oste Valerio , Barberi Filippo Maria , Bertelloni Carlo Antonio , Pedrinelli Virginia , Dell’Osso Liliana
Published: March 2022

During the 2020 first wave of the COVID-19 pandemic, general practitioners (GPs) represented the first line of primary care and were highly exposed to the pandemic risks, with a consequent risk of developing a wide range of mental health symptoms. However, scant data are still available on factors associated with a worse outcome. The aim of the present study was to investigate mental health symptoms in 139 GPs in the aftermath of the first COVID-19 national lockdown in Italy, detecting groups of subjects with different depressive, anxiety, and post-traumatic stress symptom severity. The impact of the mental health symptoms on quality of life and individual functioning were also evaluated. A cluster analysis identified three groups with mild (44.6%), moderate (35.3%), and severe psychopathological burden (20.1%). Higher symptom severity was related to younger age, fewer years in service as GPs, working in a high incidence area for the pandemic, having a relative at risk of medical complications due to COVID-19, besides more severe global functioning impairment, burnout, and secondary traumatic stress. The present findings showed that GPs, forced to perform their professional activity in extremely stressful conditions during the COVID-19 pandemic, were at high risk of developing mental health problems and a worse quality of life.
The Correlates of Academic Stress in Hong Kong
Authors: Chyu Esther Pui Yung , Chen Ji-Kang
Published: March 2022

Most previous studies have attempted to explore how different personal, familial, or school factors are linked to academic stress in Western countries. However, relatively less research has incorporated these different factors into one model to examine the most crucial correlate(s) that predict academic stress, particularly in the East Asian context, where the level of academic stress among adolescents is high. This study examined how perfectionism, social-oriented achievement motivation, parental aspiration for achievement, parent–child relationship, emphasis on academics in school, and school climate work together to predict academic stress in Hong Kong. One thousand eight hundred and four students from eight secondary schools in Hong Kong participated in this study. The results indicate that perfectionism, social-oriented achievement motivation, parent–child relationships, and emphasis on academics in school have significant associations with academic stress, while perfectionism and social-oriented achievement motivation, the two factors from the personal domain, are the dominant drivers of academic stress. In addition, these findings applied to both genders. As the significant correlates come from the personal, familial, and school domains, this study recommends multilevel interventions for decreasing the level of academic stress. In addition, this study also suggests further research directions to examine the psychosocial mechanism between the correlates and academic stress.
Knowledge, Attitude, and Practices towards Dengue Fever among University Students of Dhaka City, Bangladesh
Authors: Rahman Md Mostafizur , Khan Saadmaan Jubayer , Tanni Kamrun Nahar , Roy Tuly , Chisty Musabber Ali , Islam Md. Rakibul , Rumi Md. Alim Al Raji , Sakib Mohammed Sadman , Quader Masrur Abdul , Bhuiyan Md. Nafee-Ul-Islam , Rahman Farzana , Alam Edris , Islam Abu Reza Md. Towfiqul
Published: March 2022

Dhaka has become the worst affected city in Bangladesh regarding dengue fever (DF). A large number of university students are residing in this city with a high DF risk. This cross-sectional study was conducted to assess the DF status and responses among these students through their Knowledge, Attitude, and Practices (KAP) survey. A total of 625 students participated in an online self-reported survey. Statistical analyses were performed to assess the status and KAP regarding DF. University students from the city perceived their living places as moderately safe (45.28%) against DF, whereas about 20% reported their DF infection history. Some of these students had exemplary DF knowledge (66.72%), attitude (89.28%), and practices (68.32%). However, many of them were also observed with a lack of knowledge about this disease’s infectious behavior, recognizing Aedes mosquito breeding sites, multiple infection cases, and the risk of DF viral infection during pregnancy. Fair correlations (p < 0.001) were determined in the KAP domain. Gender, residential unit, major, and dengue-relevant subjects were found to be significant predictors (p < 0.05) of KAP level in the univariate analysis. Major subject and residential units remained significant predictors of overall KAP level in further multiple analysis. This study revealed the urgency of infectious disease-related subjects and the relevant demonstration into the university curriculum. The study’s findings can assist the university, government and non-governmental organizations, and the health and social workers to prepare a comprehensive dengue response and preparedness plan.
Asbestos and Other Hazardous Fibrous Minerals: Potential Exposure Pathways and Associated Health Risks
Authors: Berry Terri-Ann , Belluso Elena , Vigliaturo Ruggero , Gieré Reto , Emmett Edward A. , Testa Joseph R. , Steinhorn Gregor , Wallis Shannon L.
Published: March 2022

There are six elongate mineral particles (EMPs) corresponding to specific dimensional and morphological criteria, known as asbestos. Responsible for health issues including asbestosis, and malignant mesothelioma, asbestos has been well researched. Despite this, significant exposure continues to occur throughout the world, potentially affecting 125 million people in the workplace and causing thousands of deaths annually from exposure in homes. However, there are other EMPS, such as fibrous/asbestiform erionite, that are classified as carcinogens and have been linked to cancers in areas where it has been incorporated into local building materials or released into the environment through earthmoving activities. Erionite is a more potent carcinogen than asbestos but as it is seldom used for commercial purposes, exposure pathways have been less well studied. Despite the apparent similarities between asbestos and fibrous erionite, their health risks and exposure pathways are quite different. This article examines the hazards presented by EMPs with a particular focus on fibrous erionite. It includes a discussion of the global locations of erionite and similar hazardous minerals, a comparison of the multiple exposure pathways for asbestos and fibrous erionite, a brief discussion of the confusing nomenclature associated with EMPs, and considerations of increasing global mesothelioma cases.
Feasibility of Patient Navigation-Based Smoking Cessation Program in Cancer Patients
Authors: Fan Tongyao , Yingst Jessica M. , Bascom Rebecca , Tuanquin Leonard , Veldheer Susan , Branstetter Steven , Foulds Jonathan , Muscat Joshua E.
Published: March 2022

Continued smoking after a cancer diagnosis is causally associated with increased risks of all-cause and cancer-specific mortality, and of smoking-related second primary cancers. Patient navigation provides individualized assistance to address barriers to smoking cessation treatment and represents a promising bridge to smoking cessation in persons with cancer who smoke cigarettes. We conducted a single-arm interventional cohort study of current smokers identified through prospective health record screening and recruited from Penn State Cancer Institute outpatient clinics. Consented participants received two telephone intervention sessions and gain-framed messaging-based smoking cessation educational materials designed for persons with cancer. The primary study outcome was the feasibility of the patient navigation-based intervention; the secondary outcome was the engagement in smoking cessation treatment at the two-month follow-up. Of 1168 unique screened Cancer Institute patients, 134 (11.5%) were identified as current cigarette smokers. Among 67 patients approached at outpatient clinics, 24 (35.8%) were interested in participating, 12 (17.9%) were enrolled, eight (11.9%) completed the intervention sessions and study assessments, and six engaged in smoking cessation treatment. The participants expressed satisfaction with the intervention sessions (median = 8.5, scale 0–10). The low recruitment rates preclude patient navigation as a feasible method for connecting cancer patients to smoking cessation treatment resources.
Impact of Digital Device, Exercise, and Music Intervention Programs on the Cognition and Depression of the Elderly in South Korea: A Meta-Regression Analysis
Authors: Yoo Jaeeon , Oh Junga , Kim Sang-Youn , Shin Jungmin , Kim Siekyeong , Roh Changhyun
Published: March 2022

Background: This study examined the effect of digital devices, exercise, and music intervention programs for the elderly in Korea on their cognition and depression. Methods: This study selected 70 cognition programs and 46 depression programs for the elderly in Korea. This study controlled the characteristics of the programs and participants, and conducted a meta-regression analysis to estimate the intervention effect size of digital devices, exercise, and music on cognition and depression. Results: The meta-regression analysis revealed that digital device programs had a smaller effect size with respect to the improvement of cognitive functions than programs that did not use digital devices. The exercise programs had a small effect size on depression, but their effect size on cognition was not significantly different. Discussion: These findings provide implications for developing a program that combines music therapy with digital devices and exercise interventions, which can be effective in addressing both cognition and depression.
Comparison of Bioelectrical Impedance Analysis with DXA in Adolescents with Cystic Fibrosis before and after a Resistance Training Intervention
Authors: Holmes Clifton J. , Racette Susan B. , Symonds Leslie , Arbeláez Ana Maria , Cao Chao , Granados Andrea
Published: March 2022

Background: The purpose of this pilot study was to compare body composition metrics obtained by two portable bioelectrical impedance analysis (BIA) devices with dual-energy X-ray absorptiometry (DXA) among adolescents with cystic fibrosis (CF) before and after a resistance exercise training program. Methods: Participants with CF were assessed using DXA, single-frequency BIA (SFBIA), and multiple-frequency BIA (MFBIA) to quantify percent body fat (%Fat), fat mass (FM), and fat-free mass (FFM) at baseline and after a home-based resistance training intervention comprised of 36, 1 h sessions completed in 12–14 weeks. Repeated measures analysis of variance, paired samples t-tests, Cohen’s d effect sizes, and Pearson’s correlations were used to compare differences between and within methods at baseline and post-intervention. Results: Ten participants (15.8 ± 2.2 yr, 60.1 ± 15.1 kg) completed the assessments. At baseline, both SFBIA and MFBIA scales significantly underestimated %Fat and FM and overestimated FFM, with small to moderate effect sizes. Post-intervention, small, non-significant differences were found between DXA and both BIA scales for all body composition metrics. Significant changes in %Fat and FFM were observed with DXA. MFBIA displayed less constant error than SFBIA when compared to DXA for pre- and post-intervention assessments for %Fat (MFBIA: pre and post −2.8 and −0.8 vs. SFBIA: −4.6 and −2.0), FM (−0.4 and −0.4 vs. −3.0 and −1.1), and FFM (+0.8 and +0.6 vs. +3.1 and +1.3). Near-perfect correlations were observed at both time points between DXA and each BIA scale. Conclusions: Portable BIA results should be interpreted with caution, and further validation studies in CF patients are needed prior to clinical use.
Development and Feasibility of an Inpatient Cancer-Related Sarcopenia Pathway at a Major Cancer Centre
Authors: Loeliger Jenelle , Edbrooke Lara , Daly Robin M. , Stewart Jane , Bucci Lucy , Puskas Carmen , Fitzgerald Marnie , Baguley Brenton J. , Kiss Nicole
Published: March 2022

Cancer-related sarcopenia is a complex condition; however, no cancer-specific clinical model is available to guide clinical practice. This study aims to (1) develop an evidence-based care pathway for the management of cancer-related sarcopenia (“sarc-pathway”) and (2) pilot test the feasibility (reach, intervention fidelity, patient and clinician acceptability) of the sarc-pathway in an inpatient cancer ward. The sarc-pathway was developed using a care pathway format and informed by the current literature. Patients admitted to a 32-bed inpatient cancer ward were recruited to receive sarc-pathway care and the feasibility outcomes were assessed. Of the 317 participants admitted, 159 were recruited over 3.5-months (median age 61 years; 56.0% males). Participant consent was high (99.4% of those approached) and 30.2% were at risk of/had sarcopenia. The sarc-pathway screening, assessment and treatment components were delivered as intended; however, low completion of clinical assessment measures were observed for muscle mass (bioimpedance spectroscopy, 20.5%) and muscle function (5-times chair stand test, 50.0%). The sarc-pathway was demonstrated to be acceptable to patients and multidisciplinary clinicians. In an inpatient cancer ward, the sarc-pathway is a feasible and acceptable clinical model and method to deliver and adhere to the sarcopenia clinical parameters specified, albeit with further exploration of appropriate clinical assessment measures.
Prediction Model including Gastrocnemius Thickness for the Skeletal Muscle Mass Index in Japanese Older Adults
Authors: Yuguchi Satoshi , Asahi Ryoma , Kamo Tomohiko , Azami Masato , Ogihara Hirofumi
Published: March 2022

Non-invasive and easy alternative methods to indicate skeletal muscle mass index (SMI) have not been established when dual energy X-ray absorptiometry (DXA) or bioelectrical impedance analysis (BIA) cannot be performed. This study aims to construct a prediction model including gastrocnemius thickness using ultrasonography for skeletal muscle mass index (SMI). Total of 193 Japanese aged ≥65 years participated. SMI was measured by BIA, and subcutaneous fat thickness and gastrocnemius thickness in the medial gastrocnemius were measured by using ultrasonography, and age, gender and body mass index (BMI), grip strength, and gait speed were collected. The stepwise multiple regression analysis was conducted, which incorporated SMI as a dependent variable and age, gender, BMI, gastrocnemius thickness, and other factors as independent variables. Gender, BMI, and gastrocnemius thickness were included as significant factors, and the formula: SMI = 1.27 × gender (men: 1, women: 0) + 0.18 × BMI + 0.09 × gastrocnemius thickness (mm) + 1.3 was shown as the prediction model for SMI (R = 0.89, R2 = 0.8, adjusted R2 = 0.8, p < 0.001). The prediction model for SMI had high accuracy and could be a non-invasive and easy alternative method to predict SMI in Japanese older adults.
Exposure to Tobacco Smoking in Vehicles, Indoor, and Outdoor Settings in Germany: Prevalence and Associated Factors
Authors: Mlinarić Martin , Kastaun Sabrina , Kotz Daniel
Published: March 2022

Little is known on whether secondhand smoke (SHS) exposure in vehicles, indoor, and outdoor settings is similarly patterned in terms of different socio-epidemiological indicators in Germany. This study aims to estimate the current national-level prevalence and associated socio-epidemiological indicators of SHS exposure in vehicles, indoor, and outdoor settings in the German population, using current data from a representative household survey. We used cross-sectional data (N = 3928 respondents aged 14–99 years) from two waves of the DEBRA survey (German Study on Tobacco Use), conducted between January and March 2020. The reported prevalence of SHS exposure during the last seven days was 19% in vehicles, 25% in indoor settings, and 43% in outdoor settings. We found that younger age and current smoking were consistently associated with higher SHS exposure. Furthermore, people with low education were more likely to be exposed to SHS in vehicles and indoor settings than people with high education. This study found that the prevalence of SHS exposure in vehicles, indoor, and outdoor settings is a relevant feature of everyday life in Germany, especially for younger people and people with lower education, leading to potentially persistent socioeconomic and tobacco-attributable inequalities in morbidity and mortality.
Assessment of the Impact of Scientific Reports Published by EFSA and GIS on Functional Foods Newly Placed on the Market in Poland
Authors: Wróbel Kacper , Milewska Anna Justyna , Marczak Michał , Kozłowski Remigiusz
Published: March 2022

Dietary supplements are health-promoting products. The legal categorization of dietary supplements as foods does not raise concerns, but a general understanding of how they work in the human body seems to deviate from the official definition. Thus, it is necessary to establish effective methods of market control related to dietary supplements. This research aims at assessing the impact of recommendations by various food safety authorities on ingredients used in newly registered products. It probes how the proportions of utilized product ingredients were modified after the European Food Safety Authority (EFSA) and Chief Sanitary Inspector in Poland (GIS) published their recommendations. Research data on the composition of products comes from the Polish national register of dietary supplements and covers the period from 2012 to 28 November 2021. Note that 103,102 products were analysed for the presence of thirty-seven ingredients, and the joinpoint regression method was applied to assess changing trends related to the use of ingredients. As our research points out, most often, changes in the trend appeared in product ingredients for which the European Food Safety Authority and Chief Sanitary Inspector in Poland issued the recommendation of having the safest level of consumption. However, these changes seem to emerge randomly and should not be unquestionably considered as the result of the published recommendations.
Current Standards of Early Rehabilitation after Anterior Cruciate Ligament Reconstruction in German Speaking Countries—Differentiation Based on Tendon Graft and Concomitant Injuries
Authors: Memmel Clemens , Krutsch Werner , Szymski Dominik , Pfeifer Christian , Henssler Leopold , Frankewycz Borys , Angele Peter , Alt Volker , Koch Matthias
Published: March 2022

Background: Although anterior cruciate ligament reconstruction (ACLR) is a well-established procedure and is standardly performed by orthopedic surgeons all over the world, there does not seem to be a standard protocol for early rehabilitation. The purpose of this study was to give answers to the following questions: (i) Does (a) the use of a specific tendon graft, and (b) potentially additional therapy of concomitant pathologies influence surgeons’ choice of a distinct postoperative rehabilitation protocol after ACLR? (ii) To what extent do these rehabilitation recommendations differ? Methods: Retrospective analysis of currently used early rehabilitation protocols after ACLR in German-speaking countries (GER, AUT and SUI) was conducted. Rehabilitation criteria included weight bearing, range of motion (ROM), the utilization of braces, continuous passive/active motion therapy (CPM/CAM), rehabilitation training and sport-specific training. Tendon grafts were differentiated as hamstring (HAM) and bone–patellar tendon–bone grafts (BTB). Concomitant pathologies included meniscus injuries (+M) and unhappy triad injuries (+UTI). Results: Most of the surveyed protocols were differentiated according to the used tendon graft or additional therapy of concomitant injuries (ACLR-differentiated, n = 147 vs. ACLR without graft differentiation, n = 58). When comparing ACLR-HAM and ACLR-BTB, significant differences were found regarding weight bearing (p = 0.01), ROM (p = 0.05) and the utilization of braces (p = 0.03). Regarding ACLR+M, an overall significant decelerated rehabilitation could be detected. After ACLR+UTI-therapy, a significant delayed start to full weight bearing (p = 0.002) and ROM (p < 0.001) was found. Conclusions: Most orthopedic surgeons from German-speaking countries differentiate early rehabilitation after ACLR according to the tendon graft used and therapy of concomitant pathologies. No consensus about early rehabilitation after ACLR is available. However, tendencies for an accelerated rehabilitation after ACLR-BTB and a more restrained rehabilitation of multiple injured knees were detected.
Remote Support of Elderly Women Participating in Mental Health Promotion Programme during the COVID-19 Pandemic: A Single-Group Longitudinal Intervention
Authors: Juszko Karolina , Serweta Anna , Cieślik Błażej , Idzikowski Wojciech , Szczepańska-Gieracha Joanna , Gajda Robert
Published: March 2022

The aim of the study was to evaluate the longitudinal changes in mental well-being during the switch of the intervention from a personal contact to a remotely delivered Mental Health Prevention and Promotion Programme in elderly women at high risk of developing depression. The study included 70 women aged over 60 with a mean age of 72.28 years. In order to determine mental well-being, the Geriatric Depression Scale (GDS) was used at four time points: January, April, September and December 2020. A self-developed questionnaire was used to determine predictors of mental well-being. Two-way ANOVA demonstrated a lack of significant differences between the means in the GDS scores at the four data collection time points (p = 0.21). Frequent use of green areas reduced the GDS score on average by 1.52 points (p = 0.01), while owning a garden by 1.51 points (p = 0.04). The illness of a family member increased the GDS score by an average of 1.7 points (p = 0.02). No significant mood deterioration was found between January 2020 and December 2020 in the studied group of elderly women at a high risk of developing depression, which suggests that the remote support provided in the mental health promotion programme was effective.
Psychometric Properties of the Korean Version of the Environmental Health Literacy Scale
Authors: Kwak Jung-Min , Kim Ju-Hee
Published: March 2022

The environmental health literacy (EHL) scale evaluates media-specific and general EHL levels in three domains: knowledge, attitude, and behavior. This study aimed to adapt the EHL scale developed by Lichtveld et al. into the Korean language (K-EHL scale) and to verify its reliability and validity. Survey data was collected from 492 adults (19–65 years) residing in Korea. The study process included translation procedures, content validity verification, pre-testing, the actual survey, and statistical analysis for validation and selection of the final items. The scale-level content validity index was 0.92, and one item was removed. Multiple exploratory factor analyses condensed the K-EHL into 2 factors and 38 items. The “Environmental health knowledge and attitude” factor (14 items) measures information, feelings, and thoughts about environmental health. The “Environmental health behavior” factor (24 items) comprises behaviors responding to environmental health. A construct validity (criterion and discriminant validity) was verified using confirmatory factor analysis for goodness of fit (CFI = 0.901, TLI = 0.863, GFI = 0.923, NFI = 0.862, and RMSEA = 0.08). Internal consistency reliability test results showed a Cronbach’s α of 0.81 for the total items. This study is the first to introduce the EHL in Korea, and it also presents a validated evaluation tool. The K-EHL is expected to elucidate EHL levels in Korea. In the future, the EHL scale can be enhanced using this tool.
The Role of IL-22 in Wound Healing. Potential Implications in Clinical Practice
Authors: Zaharie Roxana Delia , Popa Călin , Schlanger Diana , Vălean Dan , Zaharie Florin
Published: March 2022

Wound healing is a complex process that is mediated and influenced by several cytokines, chemokines, and growth factors. Interleukin-22 (IL-22) is a cytokine that plays a critical role in tissue regeneration. Our study is a systematic review that addressed the implications of IL-22 in the healing of wounds caused by external factors. Thirteen studies were included in our review, most of them being experimental studies. Three clinical studies underlined the potential role of IL-22 in day-to-day clinical practice. IL-22 plays a central role in wound healing, stimulating the proliferation, migration, and differentiation of the cells involved in tissue repair. However, overexpression of IL-22 can cause negative effects, such as keloid scars or peritoneal adhesions. The results of the presented studies are promising, but further research that validates the roles of IL-22 in clinical practice and analyzes its potential implication in surgical healing is welcomed.
ACE2 Receptor and Its Isoform Short-ACE2 Are Expressed on Human Spermatozoa
Authors: Ramal-Sanchez Marina , Castellini Chiara , Cimini Costanza , Taraschi Angela , Valbonetti Luca , Barbonetti Arcangelo , Bernabò Nicola , Barboni Barbara
Published: March 2022

Angiotensin-converting enzyme 2 (ACE2) is a protein widely expressed in numerous cell types, with different biological roles mainly related to the renin-angiotensin system. Recently, ACE2 has been in the spotlight due to its involvement in the SARS-CoV-2 entry into cells. There are no data available regarding the expression of ACE2 and its short-ACE2 isoform at the protein level on human spermatozoa. Here, protein expression was demonstrated by western blot and the percentage of sperm displaying surface ACE2 was assessed by flow cytometry. Immunocytochemistry assays showed that full-length ACE2 was mainly expressed in sperm midpiece, while short ACE2 was preferentially distributed on the equatorial and post-acrosomal region of the sperm head. To our knowledge, this is the first study demonstrating the expression of protein ACE2 on spermatozoa. Further studies are warranted to determine the role of ACE2 isoforms in male reproduction.
Genetic Diversity and Association Analysis for Carotenoid Content among Sprouts of Cowpea (Vigna unguiculata L. Walp)
Authors: Sodedji Frejus Ariel Kpedetin , Ryu Dahye , Choi Jaeyoung , Agbahoungba Symphorien , Assogbadjo Achille Ephrem , N’Guetta Simon-Pierre Assanvo , Jung Je Hyeong , Nho Chu Won , Kim Ho-Youn
Published: March 2022

The development and promotion of biofortified foods plants are a sustainable strategy for supplying essential micronutrients for human health and nutrition. We set out to identify quantitative trait loci (QTL) associated with carotenoid content in cowpea sprouts. The contents of carotenoids, including lutein, zeaxanthin, and β-carotene in sprouts of 125 accessions were quantified via high-performance liquid chromatography. Significant variation existed in the profiles of the different carotenoids. Lutein was the most abundant (58 ± 12.8 mg/100 g), followed by zeaxanthin (14.7 ± 3.1 mg/100 g) and β-carotene (13.2 ± 2.9 mg/100 g). A strong positive correlation was observed among the carotenoid compounds (r ≥ 0.87), indicating they can be improved concurrently. The accessions were distributed into three groups, following their carotenoid profiles, with accession C044 having the highest sprout carotenoid content in a single cluster. A total of 3120 genome-wide SNPs were tested for association analysis, which revealed that carotenoid biosynthesis in cowpea sprouts is a polygenic trait controlled by genes with additive and dominance effects. Seven loci were significantly associated with the variation in carotenoid content. The evidence of variation in carotenoid content and genomic regions controlling the trait creates an avenue for breeding cowpea varieties with enhanced sprouts carotenoid content.
The Merkel Cell Polyomavirus T-Antigens and IL-33/ST2-IL1RAcP Axis: Possible Role in Merkel Cell Carcinoma
Authors: Rasheed Kashif , Moens Ugo , Policastro Benedetta , Johnsen John Inge , Koljonen Virve , Sihto Harri , Lui Weng-Onn , Sveinbjørnsson Baldur
Published: March 2022

Merkel cell polyomavirus (MCPyV) is a causal factor in Merkel cell carcinoma (MCC). The oncogenic potential is mediated through its viral oncoproteins large T-antigen (LT) and small T-antigen (sT). Cytokines produced by tumor cells play an important role in cancer pathogenesis, and viruses affect their expression. Therefore, we compared human cytokine and receptor transcript levels in virus positive (V+) and virus negative (V−) MCC cell lines. Increased expression of IL-33, a potent modulator of tumor microenvironment, was observed in V+ MCC cell lines when compared to V− MCC-13 cells. Transient transfection studies with luciferase reporter plasmids demonstrated that LT and sT stimulated IL-33, ST2/IL1RL1 and IL1RAcP promoter activity. The induction of IL-33 expression was confirmed by transfecting MCC-13 cells with MCPyV LT. Furthermore, recombinant human cytokine domain IL-33 induced activation of MAP kinase and NF-κB pathways, which could be blocked by a ST2 receptor antibody. Immunohistochemical analysis demonstrated a significantly stronger IL-33, ST2, and IL1RAcP expression in MCC tissues compared to normal skin. Of interest, significantly higher IL-33 and IL1RAcP protein levels were observed in MCC patient plasma compared to plasma from healthy controls. Previous studies have demonstrated the implication of the IL-33/STL2 pathway in cancer. Because our results revealed a T-antigens-dependent induction of the IL-33/ST2 axis, IL-33/ST2 may play a role in the tumorigenesis of MCPyV-positive MCC. Therefore, neutralizing the IL-33/ST2 axis may present a novel therapeutic approach for MCC patients.
A 69 kbp Deletion at the Berry Color Locus Is Responsible for Berry Color Recovery in Vitis vinifera L. Cultivar ‘Riesling Rot’
Authors: Röckel Franco , Moock Carina , Schwander Florian , Maul Erika , Töpfer Reinhard , Hausmann Ludger
Published: March 2022

‘Riesling Weiss’ is a white grapevine variety famous worldwide for fruity wines with higher acidity. Hardly known is ‘Riesling Rot’, a red-berried variant of ‘Riesling Weiss’ that disappeared from commercial cultivation but has increased in awareness in the last decades. The question arises of which variant, white or red, is the original and, consequently, which cultivar is the true ancestor. Sequencing the berry color locus of ‘Riesling Rot’ revealed a new VvmybA gene variant in one of the two haplophases called VvmybA3/1RR. The allele displays homologous recombination of VvmybA3 and VvmybA1 with a deletion of about 69 kbp between both genes that restores VvmybA1 transcripts. Furthermore, analysis of ‘Riesling Weiss’, ‘Riesling Rot’, and the ancestor ‘Heunisch Weiss’ along chromosome 2 using SSR (simple sequence repeat) markers elucidated that the haplophase of ‘Riesling Weiss’ was inherited from the white-berried parent variety ‘Heunisch Weiss’. Since no color mutants of ‘Heunisch Weiss’ are described that could have served as allele donors, we concluded that, in contrast to the public opinion, ‘Riesling Rot’ resulted from a mutational event in ‘Riesling Weiss’ and not vice versa.
Sturgeon Chondroitin Sulfate Restores the Balance of Gut Microbiota in Colorectal Cancer Bearing Mice
Authors: Wu Ruiyun , Shen Qian , Li Pinglan , Shang Nan
Published: March 2022

Chondroitin sulfate (CS) is a well-known bioactive substance with multiple biological functions, which can be extracted from animal cartilage or bone. Sturgeon, the largest soft bone animal with ~20% cartilage content, is a great candidate for CS production. Our recent study confirmed the role of sturgeon chondroitin sulfate (SCS) in reducing colorectal cancer cell proliferation and tumor formation. Here, we further studied the effect of SCS on modulating gut microbiome structure in colorectal cancer bearing mice. In this study, the transplanted tumor mice model was constructed to demonstrate that SCS can effectively halt the growth of transplanted colorectal tumor cells. Next, we showed that SCS significantly altered the gut microbiome, such as the abundance of Lactobacillales, Gastranaerophilales, Ruminiclostridiun_5 and Ruminiclostridiun_6. According to linear discriminant analysis (LDA) and abundance map analysis of the microbial metabolic pathways, the changes in microbial abundance led to an increase of certain metabolites (e.g., Phe, Tyr, and Gly). Fecal metabolome results demonstrated that SCS can significantly reduce the amount of certain amino acids such as Phe, Pro, Ala, Tyr and Leu presented in the feces, suggesting that SCS might inhibit colorectal cancer growth by modulating the gut microbiome and altering the production of certain amino acids. Our results revealed the therapeutic potential of SCS to facilitate treatment of colorectal cancer. This study provides insights into the development of novel food-derived therapies for colorectal cancer.
Sprague Dawley Rats Show More Severe Bone Loss, Osteophytosis and Inflammation Compared toWistar Han Rats in a High-Fat, High-Sucrose Diet Model of Joint Damage
Authors: Warmink Kelly , Rios Jaqueline L. , van Valkengoed Devin R. , Korthagen Nicoline M. , Weinans Harrie
Published: March 2022

In animal models, joint degeneration observed in response to obesogenic diet varies in nature and severity. In this study, we compare joint damage in Sprague Dawley and Wistar-Han rats in response to a high-fat, high-sucrose (HFS) diet groove model of osteoarthritis (OA). Wistar Han (n = 5) and Sprague Dawley (n = 5) rats were fed an HFS diet for 24 weeks. OA was induced 12 weeks after the diet onset by groove surgery in the right knee joint. The left knee served as a control. Outcomes were OARSI histopathology scoring, bone changes by µCT imaging, local (synovial and fat pad) and systemic (blood cytokine) inflammation markers. In both rat strains, the HFS diet resulted in a similar change in metabolic parameters, but only Sprague Dawley rats showed a large, osteoporosis-like decrease in trabecular bone volume. Osteophyte count and local joint inflammation were higher in Sprague Dawley rats. In contrast, cartilage degeneration and systemic inflammatory marker levels were similar between the rat strains. The difference in bone volume loss, osteophytosis and local inflammation suggest that both rat strains show a different joint damage phenotype and could, therefore, potentially represent different OA phenotypes observed in humans.
Neurogenesis as a Tool for Spinal Cord Injury
Authors: Havelikova Katerina , Smejkalova Barbora , Jendelova Pavla
Published: March 2022

Spinal cord injury is a devastating medical condition with no effective treatment. One approach to SCI treatment may be provided by stem cells (SCs). Studies have mainly focused on the transplantation of exogenous SCs, but the induction of endogenous SCs has also been considered as an alternative. While the differentiation potential of neural stem cells in the brain neurogenic regions has been known for decades, there are ongoing debates regarding the multipotent differentiation potential of the ependymal cells of the central canal in the spinal cord (SCECs). Following spinal cord insult, SCECs start to proliferate and differentiate mostly into astrocytes and partly into oligodendrocytes, but not into neurons. However, there are several approaches concerning how to increase neurogenesis in the injured spinal cord, which are discussed in this review. The potential treatment approaches include drug administration, the reduction of neuroinflammation, neuromodulation with physical factors and in vivo reprogramming.
Advancement of Metatranscriptomics towards Productive Agriculture and Sustainable Environment: A Review
Authors: Sharuddin Siti Suhailah , Ramli Norhayati , Yusoff Mohd Zulkhairi Mohd , Muhammad Nor Azlan Nor , Ho Li Sim , Maeda Toshinari
Published: March 2022

While chemical fertilisers and pesticides indeed enhance agricultural productivity, their excessive usage has been detrimental to environmental health. In addressing this matter, the use of environmental microbiomes has been greatly favoured as a ‘greener’ alternative to these inorganic chemicals’ application. Challenged by a significant proportion of unidentified microbiomes with unknown ecological functions, advanced high throughput metatranscriptomics is prudent to overcome the technological limitations in unfolding the previously undiscovered functional profiles of the beneficial microbiomes. Under this context, this review begins by summarising (1) the evolution of next-generation sequencing and metatranscriptomics in leveraging the microbiome transcriptome profiles through whole gene expression profiling. Next, the current environmental metatranscriptomics studies are reviewed, with the discussion centred on (2) the emerging application of the beneficial microbiomes in developing fertile soils and (3) the development of disease-suppressive soils as greener alternatives against biotic stress. As sustainable agriculture focuses not only on crop productivity but also long-term environmental sustainability, the second half of the review highlights the metatranscriptomics’ contribution in (4) revolutionising the pollution monitoring systems via specific bioindicators. Overall, growing knowledge on the complex microbiome functional profiles is imperative to unlock the unlimited potential of agricultural microbiome-based practices, which we believe hold the key to productive agriculture and sustainable environment.
Stress Granules and Acute Ischemic Stroke: Beyond mRNA Translation
Authors: Aramburu-Núñez Marta , Custodia Antía , Pérez-Mato María , Iglesias-Rey Ramón , Campos Francisco , Castillo José , Ouro Alberto , Romaus-Sanjurjo Daniel , Sobrino Tomás
Published: March 2022

Ischemic stroke is a leading cause of death and disability worldwide. Following an ischemic insult, cells undergo endoplasmic reticulum (ER) stress, which increases the ER’s protein-folding and degradative capacities and blocks the global synthesis of proteins by phosphorylating the eukaryotic translation initiation factor 2-alpha (eIF2α). Phosphorylation of eIF2α is directly related to the dynamics of stress granules (SGs), which are membraneless organelles composed of RNA-binding proteins and mRNA. SGs play a critical role in mRNA metabolism and translational control. Other translation factors are also linked to cellular pathways, including SG dynamics following a stroke. Because the formation of SGs is closely connected to mRNA translation, it is interesting to study the relationship between SG dynamics and cellular outcome in cases of ischemic damage. Therefore, in this review, we focus on the role of SG dynamics during cerebral ischemia.
Differential Oxygen Exposure Modulates Mesenchymal Stem Cell Metabolism and Proliferation through mTOR Signaling
Authors: Moniz Inês , Ramalho-Santos João , Branco Ana F.
Published: March 2022

Mesenchymal stem cells reside under precise hypoxic conditions that are paramount in determining cell fate and behavior (metabolism, proliferation, differentiation, etc.). In this work, we show that different oxygen tensions promote a distinct proliferative response and affect the biosynthetic demand and global metabolic profile of umbilical cord-mesenchymal stem cells (UC-MSCs). Using both gas-based strategies and CoCl2 as a substitute for the costly hypoxic chambers, we found that specific oxygen tensions influence the fate of UC-MSCs differently. While 5% O2 potentiates proliferation, stimulates biosynthetic pathways, and promotes a global hypermetabolic profile, exposure to <1% O2 contributes to a quiescent-like cell state that relies heavily on anaerobic glycolysis. We show that using CoCl2 as a hypoxia substitute of moderate hypoxia has distinct metabolic effects, when compared with gas-based strategies. The present study also highlights that, while severe hypoxia regulates global translation via mTORC1 modulation, its effects on survival-related mechanisms are mainly modulated through mTORC2. Therefore, the experimental conditions used in this study establish a robust and reliable hypoxia model for UC-MSCs, providing relevant insights into how stem cells are influenced by their physiological environment, and how different strategies of modulating hypoxia may influence experimental outcomes.
Role of Induced Programmed Cell Death in the Chemopreventive Potential of Apigenin
Authors: Jang Jung Yoon , Sung Bokyung , Kim Nam Deuk
Published: March 2022

The flavonoid apigenin (4′,5,7-trihydroxyflavone), which is one of the most widely distributed phytochemicals in the plant kingdom, is one of the most thoroughly investigated phenolic components. Previous studies have attributed the physiological effects of apigenin to its anti-allergic, antibacterial, antidiabetic, anti-inflammatory, antioxidant, antiviral, and blood-pressure-lowering properties, and its documented anticancer properties have been attributed to the induction of apoptosis and autophagy, the inhibition of inflammation, angiogenesis, and cell proliferation, and the regulation of cellular responses to oxidative stress and DNA damage. The most well-known mechanism for the compound’s anticancer effects in human cancer cell lines is apoptosis, followed by autophagy, and studies have also reported that apigenin induces novel cell death mechanisms, such as necroptosis and ferroptosis. Therefore, the aim of this paper is to review the therapeutic potential of apigenin as a chemopreventive agent, as well as the roles of programmed cell death mechanisms in the compound’s chemopreventive properties.
Comparative Genomics of Seasonal Senescence in Forest Trees
Authors: Batalova Anastasia Y. , Putintseva Yuliya A. , Sadovsky Michael G. , Krutovsky Konstantin V.
Published: March 2022

In the course of evolution, both flowering plants and some gymnosperms have developed such an adaptation to winter and unfavorable living conditions as deciduousness. Of particular interest is Siberian larch (Larix sibirica Ledeb.), which is the only species in the pine family (Pinaceae) with a seasonal deciduousness. New generation sequencing technologies make it possible to study this phenomenon at the genomic level and to reveal the genetic mechanisms of leaf and needle aging in angiosperms and gymnosperms. Using a comparative analysis of the genomes of evergreen and deciduous trees, it was found that the genes that control EXORDIUM LIKE 2 (EXL2) and DORMANCY-ASSOCIATED PROTEIN 1 (DRM1) proteins are most represented in Siberian larch, while an excess of genes that control proteins acting as immune receptors were found in evergreens. Orthologs from the family of genes that control leucine-rich repeat receptor-like kinases (LRR-RLK) contributed mostly to the distinction between evergreens and deciduous plants.
High-Value Chemicals from Electrocatalytic Depolymerization of Lignin: Challenges and Opportunities
Authors: Ayub Rabia , Raheel Ahmad
Published: March 2022

Lignocellulosic biomass is renewable and one of the most abundant sources for the production of high-value chemicals, materials, and fuels. It is of immense importance to develop new efficient technologies for the industrial production of chemicals by utilizing renewable resources. Lignocellulosic biomass can potentially replace fossil-based chemistries. The production of fuel and chemicals from lignin powered by renewable electricity under ambient temperatures and pressures enables a more sustainable way to obtain high-value chemicals. More specifically, in a sustainable biorefinery, it is essential to valorize lignin to enhance biomass transformation technology and increase the overall economy of the process. Strategies regarding electrocatalytic approaches as a way to valorize or depolymerize lignin have attracted significant interest from growing scientific communities over the recent decades. This review presents a comprehensive overview of the electrocatalytic methods for depolymerization of lignocellulosic biomass with an emphasis on untargeted depolymerization as well as the selective and targeted mild synthesis of high-value chemicals. Electrocatalytic cleavage of model compounds and further electrochemical upgrading of bio-oils are discussed. Finally, some insights into current challenges and limitations associated with this approach are also summarized.