bluuebunny/crossref_metadata_2025 huggingface dataset:
Scaling Laws for Partially Developed Turbulence
Authors: Hsu Abigail , Kaufman Ryan , Glimm James
Published: January 2022

We formulate multifractal models for velocity differences and gradients which describe the full range of length scales in turbulent flow, namely: laminar, dissipation, inertial, and stirring ranges. The models subsume existing models of inertial range turbulence. In the localized ranges of length scales in which the turbulence is only partially developed, we propose multifractal scaling laws with scaling exponents modified from their inertial range values. In local regions, even within a fully developed turbulent flow, the turbulence is not isotropic nor scale invariant due to the influence of larger turbulent structures (or their absence). For this reason, turbulence that is not fully developed is an important issue which inertial range study can not address. In the ranges of partially developed turbulence, the flow can be far from universal, so that standard inertial range turbulence scaling models become inapplicable. The model proposed here serves as a replacement. Details of the fitting of the parameters for the τp and ζp models in the dissipation range are discussed. Some of the behavior of ζp for larger p is unexplained. The theories are verified by comparing to high resolution simulation data.
Boosting Electrochemical Carbon Dioxide Reduction on Atomically Dispersed Nickel Catalyst
Authors: Hao Qi , Liu Dong-Xue , Deng Ruiping , Zhong Hai-Xia
Published: January 2022

Single-atom catalysts (SACs) with metal–nitrogen (M–N) sites are one of the most promising electrocatalysts for electrochemical carbon dioxide reduction (ECO2R). However, challenges in simultaneously enhancing the activity and selectivity greatly limit the efficiency of ECO2R due to the improper interaction of reactants/intermediates on these catalytic sites. Herein, we report a carbon-based nickel (Ni) cluster catalyst containing both single-atom and cluster sites (NiNx-T, T = 500–800) through a ligand-mediated method and realize a highly active and selective electrocatalytic CO2R process. The catalytic performance can be regulated by the dispersion of Ni–N species via controlling the pyrolysis condition. Benefitting from the synergistic effect of pyrrolic-nitrogen coordinated Ni single-atom and cluster sites, NiNx-600 exhibits a satisfying catalytic performance, including a high partial current density of 61.85 mA cm−2 and a high turnover frequency (TOF) of 7,291 h−1 at −1.2 V vs. RHE, and almost 100% selectivity toward carbon monoxide (CO) production, as well as good stability under 10 h of continuous electrolysis. This work discloses the significant role of regulating the coordination environment of the transition metal sites and the synergistic effect between the isolated single-site and cluster site in enhancing the ECO2R performance.
Recovery of Recombinant Canine Distemper Virus That Expresses CPV-2a VP2: Uncovering the Mutation Profile of Recombinant Undergoing 50 Serial Passages In Vitro
Authors: Liu Fuxiao , Lin Jiahui , Wang Qianqian , Zhang Youming , Shan Hu
Published: January 2022

Canine distemper and canine parvoviral enteritis are infections caused by the canine distemper virus (CDV) and canine parvovirus type 2 (CPV-2), respectively. They are two common infectious diseases that cause high morbidity and mortality in affected dogs. Combination vaccines have been broadly used to protect dogs from infections of CDV, CPV-2, and other viruses. VP2 is the most abundant protein of the CPV-2 capsid. It elicits potent immunity in animals and, therefore, is widely used for designing subunit antigen-based vaccines. In this study, we rescued a recombinant CDV (QN vaccine strain) using reverse genetics. The recombinant CDV (rCDV-VP2) was demonstrated to express stably the VP2 in cells for at least 33 serial passages in vitro. Unfortunately, a nonsense mutation was initially identified in the VP2 open reading frame (ORF) at passage-34 (P34) and gradually became predominant in rCDV-VP2 quasispecies with passaging. Neither test strip detection nor indirect immunofluorescence assay demonstrated the expression of the VP2 at P50. The P50 rCDV-VP2 was subjected to next-generation sequencing, which totally identified 17 single-nucleotide variations (SNVs), consisting of 11 transitions and 6 transversions. Out of the 17 SNVs, 1 and 9 were identified as nonsense and missense mutations, respectively. Since the nonsense mutation arose in the VP2 ORF as early as P34, an earlier rCDV-VP2 progeny should be selected for the vaccination of animals in future experiments.
Household Customers’ Cost of Interruption: Survey Study on Summer Electricity Peak of Xi’an City
Authors: Zhao Yuan , Yu Weihua , Guo Dingwei , He Xiaoping
Published: January 2022

In light of China’s Carbon Neutrality Target and facing the fluctuating pressure of power supply brought on by new energy intermittent power generation, it is urgent to mobilize a large number of residential flexible loads that can respond instantaneously to mitigate peak–valley difference. Under a framework of demand-side management (DSM) and utility analysis, we empirically investigate customers’ costs from interrupting typical electrical terminals at the household level. Specifically, by using the contingent valuation method (CVM), we explore the factors that affect households’ Willingness to Accept (WTA) of voluntarily participating in the interruption management during the summer electricity peak and estimate the distribution of households’ WTA values. We find that given the value of WTA, households’ participation rate in the interruption management significantly decreases with the increase in interruption duration and varies with the type of terminal appliance that is on direct interruption management. Moreover, the majority of households are willing to participate in the interruption management even if the compensation amount is low. The factors that determine households’ WTA and the size of their influences vary with the type of electrical terminal. The results imply that differentiating the terminal electricity market and accurately locking on the target terminals by considering the household heterogeneity can reduce the household welfare losses arising from DSM.
Vertical Stratification of Insect Species Developing in Water-Filled Tree Holes
Authors: Gossner Martin M. , Petermann Jana S.
Published: January 2022

Forest ecosystems have a distinct vertical dimension, but the structuring of communities in this three-dimensional space is not well understood. Water-filled tree holes are natural microcosms structured in metacommunities. Here, we used these microcosms as model systems to analyze how insect communities and the occurrence and abundance of individual species are influenced by biotic and abiotic microhabitat characteristics, the vertical position of the tree hole, and stand-scale habitat availability. We found that both the characteristics of water-filled tree holes and their insect communities differ significantly between canopy and ground level. Individual insect species showed contrasting responses to the vertical position of the tree holes when important environmental factors at the stand and the tree-hole scale were considered. While some species, such as the mosquito Aedes geniculatus and the beetle Prionocyphon serricornis, decreased in abundance with increasing tree-hole height, the biting midge Dasyhelea sp., the non-biting midge Metriocnemus cavicola and the hoverfly Myiatropa florea increased in abundance. Our results suggest that vertical stratification in forests is most likely driven not only by variation in tree-hole microhabitat properties, i.e., niche separation, but also by individual species traits, such as adult dispersal propensity, food preferences and mating behavior of adult stages, and interspecific competition of larval stages. Therefore, communities of insect species developing in tree holes are likely structured by competition–colonization trade-offs predicted by metacommunity theory.
From Ecosystem Observation to Environmental Decision-Making: Model-Data Fusion as an Operational Tool
Authors: Smallman Thomas Luke , Milodowski David Thomas , Williams Mathew
Published: January 2022

Supporting a transition to net-zero carbon (C) emissions is a key component of international action to avoid dangerous climate change. Science has outlined potential routes to net-zero, which include using nature-based solutions to grow C sinks and diminish sources linked to land use and land use change. However, decision-makers are challenged by ongoing climate change and the complexity of the biosphere, interacting with socio-economic constraints. Decision-makers need science-based, but easy to use, tools to understand the current and potential future states of the terrestrial C-cycle, and its sensitivity to their decisions. These tools must provide clear uncertainty estimates to help take account of risks, must be flexible enough to be updated as new data become available, and simple enough to be deployed broadly. We argue that model-data fusion approaches, combining the systemic ecological theory embedded in intermediate complexity models with an ever-expanding collection of ecosystem observations from field and remote sensing campaigns, provide the scientific means to address each of these challenges and therefore facilitate management decisions as we face an uncertain future.
Natural Killer Cells in Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis - A Review of the Literature
Authors: Fuchs Sina , Scheffschick Andrea , Gunnarsson Iva , Brauner Hanna
Published: January 2022

Anti-neutrophil cytoplasmic antibody (ANCA)- associated vasculitis (AAV) is a group of systemic autoimmune diseases characterized by inflammation of small- and medium-sized vessels. The three main types of AAV are granulomatosis with polyangiitis (GPA), microscopic polyangiitis (MPA) and eosinophilic granulomatosis with polyangiitis (EGPA). A growing number of studies focus on natural killer (NK) cells in AAV. NK cells are innate lymphoid cells with important roles in anti-viral and anti-tumor defense, but their roles in the pathogenesis of autoimmunity is less well established. In this review, we will present a summary of what is known about the number, phenotype and function of NK cells in patients with AAV. We review the literature on NK cells in the circulation of AAV patients, studies on tissue resident NK cells and how the treatment affects NK cells.
The Role of Advanced Imaging in Gout Management
Authors: Li Shuangshuang , Xu Guanhua , Liang Junyu , Wan Liyan , Cao Heng , Lin Jin
Published: January 2022

Gout is a common form of inflammatory arthritis where urate crystals deposit in joints and surrounding tissues. With the high prevalence of gout, the standardized and effective treatment of gout is very important, but the long-term treatment effect of gout is not satisfied because of the poor adherence in patients to the medicines. Recently, advanced imaging modalities, including ultrasonography (US), dual-energy computed tomography (DECT), and magnetic resonance imaging (MRI), attracted more and more attention for their role on gout as intuitive and non-invasive tools for early gout diagnosis and evaluation of therapeutic effect. This review summarized the role of US, DECT, and MRI in the management of gout from four perspectives: hyperuricemia, gout attacks, chronic gout, and gout complications described the scoring systems currently used to quantify disease severity and discussed the challenges and limitations of using these imaging tools to assess response to the gout treatment.
Development of a Seafloor Community Classification for the New Zealand Region Using a Gradient Forest Approach
Authors: Stephenson Fabrice , Rowden Ashley A. , Brough Tom , Petersen Grady , Bulmer Richard H. , Leathwick John R. , Lohrer Andrew M. , Ellis Joanne I. , Bowden David A. , Geange Shane W. , Funnell Greig A. , Freeman Debbie J. , Tunley Karen , Tellier Pierre , Clark Dana E. , Lundquist Carolyn J. , Greenfield Barry L. , Tuck Ian D. , Mouton Theophile L. , Neill Kate F. , Mackay Kevin A. , Pinkerton Matt H. , Anderson Owen F. , Gorman Richard M. , Mills Sadie , Watson Stephanie , Nelson Wendy A. , Hewitt Judi E.
Published: January 2022

To support ongoing marine spatial planning in New Zealand, a numerical environmental classification using Gradient Forest models was developed using a broad suite of biotic and high-resolution environmental predictor variables. Gradient Forest modeling uses species distribution data to control the selection, weighting and transformation of environmental predictors to maximise their correlation with species compositional turnover. A total of 630,997 records (39,766 unique locations) of 1,716 taxa living on or near the seafloor were used to inform the transformation of 20 gridded environmental variables to represent spatial patterns of compositional turnover in four biotic groups and the overall seafloor community. Compositional turnover of the overall community was classified using a hierarchical procedure to define groups at different levels of classification detail. The 75-group level classification was assessed as representing the highest number of groups that captured the majority of the variation across the New Zealand marine environment. We refer to this classification as the New Zealand “Seafloor Community Classification” (SCC). Associated uncertainty estimates of compositional turnover for each of the biotic groups and overall community were also produced, and an added measure of uncertainty – coverage of the environmental space – was developed to further highlight geographic areas where predictions may be less certain owing to low sampling effort. Environmental differences among the deep-water New Zealand SCC groups were relatively muted, but greater environmental differences were evident among groups at intermediate depths in line with well-defined oceanographic patterns observed in New Zealand’s oceans. Environmental differences became even more pronounced at shallow depths, where variation in more localised environmental conditions such as productivity, seafloor topography, seabed disturbance and tidal currents were important differentiating factors. Environmental similarities in New Zealand SCC groups were mirrored by their biological compositions. The New Zealand SCC is a significant advance on previous numerical classifications and includes a substantially wider range of biological and environmental data than has been attempted previously. The classification is critically appraised and considerations for use in spatial management are discussed.
Changing Ecological Conditions in the Marine Environment Generate Different Microbial Food Web Structures in a Repeatable Manner
Authors: Šolić Mladen , Šantić Danijela , Šestanović Stefanija , Kušpilić Grozdan , Matić Frano , Vrdoljak Tomaš Ana , Jozić Slaven , Bojanić Natalia , Ninčević Gladan Živana
Published: January 2022

The mechanisms responsible for the development of various structural and functional features of the microbial food web (MFW) and their dynamics at spatial and temporal scales, which are important for predicting their responses to future environmental changes, are largely unknown. More than 3000 datasets of environmental and microbial variables collected over a decade on a seasonal and large spatial scale in the Adriatic Sea were analyzed. The sets of environmental variables were classified into four clusters (representing different environmental states) using Neural Gas analysis and the differences in MFW structure between the clusters were analyzed. Different variants of MFW evolve in the different clusters in terms of the abundance of MFW components, their ratios, growth and grazing rates, predator preference in prey selection, the strength of predator-prey interaction, and the relative importance of top-down and bottom-up control. However, these clusters are neither spatially nor temporally fixed; rather, the area studied represents a mosaic of different environmental conditions that alternate from one state to another on a time scale. In each of the environmental states, a distinct structure of MFW develops that shows consistent and repeatable changes that strictly follow the switching in environmental conditions from one state to another.
Endoscopic Management of Complex Colorectal Polyps: Current Insights and Future Trends
Authors: Mann Rupinder , Gajendran Mahesh , Umapathy Chandraprakash , Perisetti Abhilash , Goyal Hemant , Saligram Shreyas , Echavarria Juan
Published: January 2022

Most colorectal cancers arise from adenomatous polyps and sessile serrated lesions. Screening colonoscopy and therapeutic polypectomy can potentially reduce colorectal cancer burden by early detection and removal of these polyps, thus decreasing colorectal cancer incidence and mortality. Most endoscopists are skilled in detecting and removing the vast majority of polyps endoscopically during a routine colonoscopy. Polyps can be considered “complex” based on size, location, morphology, underlying scar tissue, which are not amenable to removal by conventional endoscopic polypectomy techniques. They are technically more challenging to resect and carry an increased risk of complications. Most of these polyps were used to be managed by surgical intervention in the past. Rapid advancement in endoscopic resection techniques has led to a decreasing role of surgery in managing these complex polyps. These endoscopic resection techniques do require an expert in the field and advanced equipment to perform the procedure. In this review, we discuss various advanced endoscopic techniques for the management of complex polyps.
Oxidative Stress in Intestinal Ischemia-Reperfusion
Authors: Li Guangyao , Wang Shuang , Fan Zhe
Published: January 2022

Ischemia-reperfusion (I/R) injury is a manifestation of tissue or organ damage that is followed by ischemia and exacerbated by the return of blood flow to a previously damaged tissue or organ. The intestines are one of the most sensitive tissues and organs to I/R injury. Moreover, the adverse consequences of intestinal I/R (II/R) injury are not limited to the intestine itself and can also lead to damage of the distant tissues and organs. The mechanism of II/R is extremely complex and oxidative stress is the key link in the pathogenesis of II/R injury. This study summarizes the roles of oxidative stress and its signaling pathways involved in II/R. The signaling pathways that mitigate II/R injury include the nuclear factor erythroid-related factor 2 (Nrf2)-mediated signaling pathway, Wnt/β-catenin pathway, and phosphatidylinositol kinase 3 (PI3K)/Akt pathway; those that aggravate II/R injury include the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway, Toll-like receptor (TLR) receptor-mediated signaling pathway, protein kinase CβII (PKCβII)/p66shc pathway, and microRNA (miRNA)/p66shc pathway; the effect of miRNA on related pathways and mitochondrial DNA translocation. The aforementioned pathways provide new ideas for further exploring the occurrence and development of II/R and more effective treatments for II/R injury.
Effectiveness of Baricitinib in Refractory Seronegative Rheumatoid Arthritis and Uveitis: A Case Report
Authors: Kaneko Yutaka , Murakami Takanori , Nishitsuka Koichi , Takakubo Yuya , Takagi Michiaki , Yamashita Hidetoshi
Published: January 2022

Baricitinib is a Janus kinase (JAK) inhibitor used to treat refractory rheumatoid arthritis and blocks the subtypes JAK1 and JAK2. A 35-year-old man with seronegative rheumatoid arthritis complicated by bilateral severe non-granulomatous panuveitis was resistant to steroid treatment, multiple conventional disease-modifying antirheumatic drugs (methotrexate and salazosulfapyridine), and TNF-α inhibitors (adalimumab and infliximab). Therefore, the TNF-α inhibitors were switched to baricitinib to decrease the activity of systemic arthritis. Along with the amelioration of inflammatory activity in seronegative rheumatoid arthritis, the inflammatory activity of uveitis was decreased. Vitreous opacity, serous retinal detachment, and anterior chamber cells showed improvement. Baricitinib was effective not only in refractory systemic arthritis but also in uveitis, which may provide a new treatment option for patients with refractory uveitis.
An Overview on Actual Knowledge About Immunohistochemical and Molecular Features of Vitality, Focusing on the Growing Evidence and Analysis to Distinguish Between Suicidal and Simulated Hanging
Authors: Maiese Aniello , Del Duca Fabio , Santoro Paola , Pellegrini Lavinia , De Matteis Alessandra , La Russa Raffaele , Frati Paola , Fineschi Vittorio
Published: January 2022

In forensic practice, the pathologist is often asked to determine whether a hanging was committed as suicide or as a simulated hanging (when a dead body is suspended after death). When exterior evidence of violence is absent and the crime scene investigation fails to identify useful proof, it is nearly impossible to tell whether the dead body was suspended or not. As a result, determining whether the ligature mark was created during life or not should rely on the research and demonstration of vital reactions on the ligature mark. The main purpose of this review article is to provide a summary of current knowledge about the histological and immunohistochemical characteristics of vitality in hanging. The authors also aim to identify the most significant vitality markers on ligature marks for further scientific validation and to propose a standardized diagnostic protocol for hanging. The study was conducted according to the Preferred Reporting Items for Systematic Review (PRISMA) Protocol. Relevant scientific papers were found from PubMed up to April 2021, using the following keywords: hanging AND skin AND vitality. Three main points were studied: ligature mark dehydration, immunological response to mechanical injury, and apoptosis induction as a result of the previous points. An increase in apoptosis is evident in the ligature mark (due to physical and chemical processes involved), as demonstrated by FLICE-inhibitory protein (FLIP) depletion. Immunohistochemical detection of Aquaporin 3 (AQP3) and increase in the concentration of different electrolytes rely solely on ligature mark dehydration. Also, microRNAs (MiRNAs) could become reliable forensic biomarkers for ligature mark vitality diagnosis in the near future. To ensure high reliability in court cases, forensic investigation in hanging should rely on modern and proven markers, even a mix of several markers.
Response to Vaccination Against SARS-CoV-2 in Patients With Antineutrophil Cytoplasmic Antibody-Associated Vasculitis With Renal Involvement
Authors: Carruthers Jack E. , Wells James , Gupta Arun , Kallon Delordson , Cox Amber , Pina Neuza , Yaqoob Muhammad Magdi , Rajakariar Ravindra
Published: January 2022

Background

Patients with anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV) present with multisystem disease including renal impairment. The treatment for AAV involves a high burden of immunosuppression. Patients with renal involvement are treated especially intensively. As a result, we identified these patients as being potentially at high risk of failure to seroconvert to COVID-19 vaccination.

Methods

We collected data on seroconversion response rates to COVID-19 vaccination in a multi-ethnic cohort of patients with AAV and renal involvement treated at a busy tertiary nephrology centre as part of a retrospective review of patient notes. Blood samples were taken following vaccination with either Pfizer or Astra-Zeneca COVID-19 vaccines and median fluorescence intensity was measured using the validated MULTICOV-Ab Magnetic LuminexⓇ Assay. We also evaluated whether seroconversion was affected by immunosuppression regimen.

Results

81 patients were included. The mean age was 62, and there were 49 (60%) females. 55 patients had a blood test after the first dose; 46 after the second dose. Patients were in remission with a median BVAS of 0 (IQR 2). Seroconversion after the first dose with either vaccine was 35/55 (63.6%). After the second it was 38/46 (82.6%). Subgroup analyses revealed a trend to impaired seroconversion in non-white versus white patients (77.8 vs. 81.7% (p = 0.69) after the first dose of vaccine and in those treated with Rituximab in the last 12 months (73.3 vs. 87.1%, p = 0.41).

Conclusions

These data offer real-world evidence of lower seroconversion in response to vaccination with one dose in patients with AAV and renal involvement than the general UK population. After two doses, seroconversion is in line with national data. These data provide a rationale for hospital-led identification of patients most at risk of COVID-19 and underscore the importance of local connexions between hospitals and their communities. These data provide further support for targeting booster vaccination programmes to vulnerable patient cohorts. They add to the growing evidence of reduced seroconversion in response to vaccination in patients with renal disease of any cause.
Genome Recombination-Mediated tRNA Up-Regulation Conducts General Antibiotic Resistance of Bacteria at Early Stage
Authors: Fang Huiying , Zeng Guandi , Gu Wei , Wang Yubin , Zhao Jing , Zheng Tingkai , Xu Lina , Liu Yutong , Zhang Jinning , Sun Xuesong , Zhang Gong
Published: January 2022

Bacterial antibiotic resistance sets a great challenge to human health. It seems that the bacteria can spontaneously evolve resistance against any antibiotic within a short time without the horizontal transfer of heterologous genes and before accumulating drug-resistant mutations. We have shown that the tRNA-mediated translational regulation counteracts the reactive oxygen species (ROS) in bacteria. In this study, we demonstrated that isolated and subcultured Escherichia coli elevated its tRNAs under antibiotic stress to rapidly provide antibiotic resistance, especially at the early stage, before upregulating the efflux pump and evolving resistance mutations. The DNA recombination system repaired the antibiotic-induced DNA breakage in the genome, causing numerous structural variations. These structural variations are overrepresented near the tRNA genes, which indicated the cause of tRNA up-regulation. Knocking out the recombination system abolished the up-regulation of tRNAs, and coincidently, they could hardly evolve antibiotic resistance in multiple antibiotics, respectively. With these results, we proposed a multi-stage model of bacterial antibiotic resistance in an isolated scenario: the early stage (recombination—tRNA up-regulation—translational regulation); the medium stage (up-regulation of efflux pump); the late stage (resistant mutations). These results also indicated that the bacterial DNA recombination system and tRNA could be targeted to retard the bacterial spontaneous drug resistance.
Broad Spectrum Algae Compounds Against Viruses
Authors: Reis Jacqueline Graff , Cadamuro Rafael Dorighello , Cabral Ariadne Cristiane , Thaís da Silva Izabella , Rodríguez-Lázaro David , Fongaro Gislaine
Published: January 2022

The pharmaceutical industry is currently trying to develop new bioactive compounds to inactivate both enveloped and non-enveloped viruses for therapeutic purposes. Consequently, microalgal and macroalgal bioactive compounds are being explored by pharmaceutical, as well as biotechnology and food industries. In this review, we show how compounds produced by algae include important candidates for viral control applications. We discuss their mechanisms of action and activity against enveloped and non-enveloped viruses, including those causing infections by enteric, parenteral, and respiratory routes. Indeed, algal products have potential in human and animal medicine.
Beyond to the Stable: Role of the Insertion Sequences as Epidemiological Descriptors in Corynebacterium striatum
Authors: Leyton-Carcaman Benjamín , Abanto Michel
Published: January 2022

In recent years, epidemiological studies of infectious agents have focused mainly on the pathogen and stable components of its genome. The use of these stable components makes it possible to know the evolutionary or epidemiological relationships of the isolates of a particular pathogen. Under this approach, focused on the pathogen, the identification of resistance genes is a complementary stage of a bacterial characterization process or an appendix of its epidemiological characterization, neglecting its genetic components’ acquisition or dispersal mechanisms. Today we know that a large part of antibiotic resistance is associated with mobile elements. Corynebacterium striatum, a bacterium from the normal skin microbiota, is also an opportunistic pathogen. In recent years, reports of infections and nosocomial outbreaks caused by antimicrobial multidrug-resistant C. striatum strains have been increasing worldwide. Despite the different existing mobile genomic elements, there is evidence that acquired resistance genes are coupled to insertion sequences in C. striatum. This perspective article reviews the insertion sequences linked to resistance genes, their relationship to evolutionary lineages, epidemiological characteristics, and the niches the strains inhabit. Finally, we evaluate the potential of the insertion sequences for their application as a descriptor of epidemiological scenarios, allowing us to anticipate the emergence of multidrug-resistant lineages.
Clinical Characteristics of Minor Hallucinations in Chinese Parkinson's Disease Patients
Authors: Zhang Yu , Zhang Guo yong , Zhu Xiao bo , Zhang Zi en , Gan Jing , Liu Zhen guo
Published: January 2022

Background

Psychotic symptoms are common in Parkinson's disease (PD). However, the clinical characteristics of PD psychosis (PDP) have been rarely reported in Chinese PD patients. We aimed to categorize PDP in a PD cohort and its relationship to other clinical characteristics.

Methods

A total of 149 Chinese PD patients were consecutively enrolled, and idiopathic PD patients were recruited in the study. The symptoms of PDP were assessed with the enhanced Scale for the Assessment of Positive Symptoms in PD. Then, the patients were classified into a PD-control group, isolated minor hallucination (MH) group, and complex MH group, and clinical and demographic data of different groups were compared.

Results

Parkinson's disease psychosis was present in 40.3% (60/149) of our patients. The most common PDPs were MHs, present in 32.9% (49 of 149) of the cohort. Compared to patients without MHs, patients with MHs were older, had a longer disease duration, a higher levodopa equivalent daily dose, more severe motor symptoms, dyskinesia, a higher rate of rapid eye movement sleep behavior disorders, frontal lobe function impairments, and a higher percentage of cognitive impairment. Logistic regression analysis showed that advanced Hoehn-Yahr stage [odds ratio (OR): 2.697, p = 0.007)] and frontal lobe function impairment (OR: 0.684, p = 0.003) were independent risk factors for MHs.

Conclusion

MHs were frequent non-motor symptoms in PD patients. It was associated with increased motor and non-motor symptom burdens and reduced quality of life. MHs have been called “minor,” but they have major clinical and prognostic implications and need early screening.
Early Microcirculatory Hemodynamic Changes Are Correlated With Functional Outcomes at Discharge in Patients With Aneurysmal SAH
Authors: Wen Lili , Zhou Longjiang , Wu Qi , Tang Xiaoyu , Ge Jiajia , Zhou Xiaoming , Zhang Xin
Published: January 2022

Purpose

The technique of color-coding blood flow analysis was used to explore the correlation between the microcirculatory hemodynamic changes on digital subtraction angiography (DSA) images in patients with aneurysmal subarachnoid hemorrhage (SAH) at the early stage and functional outcomes at discharge.

Methods

Data of 119 patients who underwent DSA examination due to SAH were retrospectively analyzed. The following hemodynamic parameters of the four region of interests (ROIs) [an ophthalmic segment of the internal carotid artery (ICA), frontal and parietal lobe, and superior sagittal sinus] were analyzed: the time-to-peak (TTP), the area under the curve (AUC), the full width at half maximum (FWHM), mean transit time (MTT), and circulation time. Multifactor regression analysis was performed to explore the correlation between the hemodynamic parameters and functional outcomes in patients at discharge.

Results

Of 119 patients with SAH, good and poor outcomes were found in 83 (69.7%) and 36 (30.3%) patients, respectively. The hemodynamic parameters including the FWHM, relative TTP (rTTP), and circulation time were significantly correlated with the Hunt–Hess grade (p < 0.005, p = 0.03, and p < 0.005) and the World Federation of Neurological Societies Scale grade (p < 0.005, p = 0.02, and p = 0.01). The FWHM was significantly prolonged with the increase of modified Fisher grade (p = 0.02). The multifactor analysis showed that the FWHM [odds ratio (OR) 17.56, 95% CI: 1.13–272.03, p = 0.04] was an independent risk factor predicting the functional outcomes in patients at discharge.

Conclusion

The technique of color-coding blood flow analysis could be suitable for the qualified evaluation of disease conditions at an early stage of SAH as well as the prediction of outcomes.
Survival Patterns of Human Prion Diseases in Spain, 1998–2018: Clinical Phenotypes and Etiological Clues
Authors: Tejedor-Romero Laura , López-Cuadrado Teresa , Almazán-Isla Javier , Calero Miguel , García López Fernando J. , de Pedro-Cuesta Jesús
Published: January 2022

Background

Human transmissible spongiform encephalopathies (TSEs) are a group of fatal neurodegenerative disorders of short duration. There are few studies on TSE survival. This study sought to analyze the survival and related factors of a TSE patient cohort, based on a nationwide surveillance system in Spain.

Methods

Survival analyses were performed on 1,530 cases diagnosed across the period 1998–2018 in Spain. We calculated median survival times and plotted survival curves using the Kaplan–Meier method for all cases and for sporadic TSE (sTSE) and genetic TSE (gTSE). Crude and adjusted Cox proportional hazard models were used to identify variables associated with shorter survival.

Findings

Median age at onset decreased from the sporadic forms to gTSE and, lastly, to acquired TSE. Overall median and interquartile range (IQR) survival time was 5.2 (IQR, 3.0–11.7) months and 4.9 (IQR, 2.8–10.8) months in sporadic cases and 9 (IQR, 4.9 to over 12) months in genetic cases, p < 0.001. Male sex, older age at onset, presence of 14-3-3 protein, typical MRI, and MM and VV polymorphisms at codon 129 were associated with shorter survival. gTSE showed higher survival in crude comparisons but not after adjustment.

Interpretation

TSE survival in Spain replicates both the magnitude of that shown and the TSE entity-specific population patterns observed in Western countries but differs from features described in Asian populations, such as the Japanese. The reduction in differences in survival between gTSE and sTSE on adjusting for covariates and international patterns might support the view that gTSE and sTSE share causal and pathophysiological features.
Cognitive Implications of Correlated Structural Network Changes in Schizophrenia
Authors: Jensen Dawn M. , Zendrehrouh Elaheh , Calhoun Vince , Turner Jessica A.
Published: January 2022

Background

Schizophrenia is a brain disorder characterized by diffuse, diverse, and wide-spread changes in gray matter volume (GM) and white matter structure (fractional anisotropy, FA), as well as cognitive impairments that greatly impact an individual’s quality of life. While the relationship of each of these image modalities and their links to schizophrenia status and cognitive impairment has been investigated separately, a multimodal fusion via parallel independent component analysis (pICA) affords the opportunity to explore the relationships between the changes in GM and FA, and the implications these network changes have on cognitive performance.

Methods

Images from 73 subjects with schizophrenia (SZ) and 82 healthy controls (HC) were drawn from an existing dataset. We investigated 12 components from each feature (FA and GM). Loading coefficients from the images were used to identify pairs of features that were significantly correlated and showed significant group differences between HC and SZ. MANCOVA analysis uncovered the relationships the identified spatial maps had with age, gender, and a global cognitive performance score.

Results

Three component pairs showed significant group differences (HC > SZ) in both gray and white matter measurements. Two of the component pairs identified networks of gray matter that drove significant relationships with cognition (HC > SZ) after accounting for age and gender. The gray and white matter structural networks identified in these three component pairs pull broadly from many regions, including the right and left thalamus, lateral occipital cortex, multiple regions of the middle temporal gyrus, precuneus cortex, postcentral gyrus, cingulate gyrus/cingulum, lingual gyrus, and brain stem.

Conclusion

The results of this multimodal analysis adds to our understanding of how the relationship between GM, FA, and cognition differs between HC and SZ by highlighting the correlated intermodal covariance of these structural networks and their differential relationships with cognitive performance. Previous unimodal research has found similar areas of GM and FA differences between these groups, and the cognitive deficits associated with SZ have been well documented. This study allowed us to evaluate the intercorrelated covariance of these structural networks and how these networks are involved the differences in cognitive performance between HC and SZ.
The Framework for Responsible Research With Australian Native Plant Foods: A Food Chemist's Perspective
Authors: Fyfe Selina , Smyth Heather E. , Schirra Horst Joachim , Rychlik Michael , Sultanbawa Yasmina
Published: January 2022

Australia is a rich source of biodiverse native plants that are mostly unstudied by western food science despite many of them being ethnofoods of Australian Indigenous people. Finding and understanding the relevant policy and legal requirements to scientifically assess these plants in a responsible way is a major challenge for food scientists. This work aims to give an overview of what the legal and policy framework is in relation to food chemistry on Australian native plant foods, to clarify the relationships between the guidelines, laws, policies and ethics and to discuss some of the challenges they present in food chemistry. This work provides the framework of Indigenous rights, international treaties, federal and state laws and ethical guidelines including key legislation and guidelines. It discusses the specific areas that are applicable to food chemistry: the collection of plant foods, the analysis of the samples and working with Indigenous communities. This brief perspective presents a framework that can be utilized by food chemists when developing responsible research involving plant foods native to northern Australia and can help them understand some of the complexity of working in this research area.
High-Protein Concentrated Pro-Yogurt (Pro-WPI) Enriched With Whey Protein Isolate Improved Athletic Anemia and Performance in a Placebo-Controlled Study
Authors: Gomaa Mohamed A. E. , Allam Marwa G. , Haridi Abdallah A. I. M. , Eliwa Alaa-Eldin M. , Darwish Amira M. G.
Published: January 2022

Upcoming developments are attracting attention to both high-protein and probiotics supplementation for the sports community to promote good health and exercise performance. This study aimed at the production of high-protein concentrated pro-yogurt (Pro-WPI) enriched with 10 and 20% whey protein isolate (WPI) and investigation of the response of daily consumption on anthropometric, hematology parameters, and athletic performance in parallel with safety consideration assessment. Twenty-four athletes (19.6 ± 1.45 years; 175.96 ± 5.24 cm; 73.16 ± 8.65 kg) were participated in a randomized placebo-control study. They consumed Pro-WPI products with 10 (T1) and 20% (T2) WPI for treatments G1 (Pro-WPI30) and G2 (Pro-WPI60), respectively, 3 times per day/5 days per week/9 weeks. The taste of Pro-WPI products was sour and cheesy, while mouthfeel was described as soft and thick because of the increased protein content in T1 and T2 (14.15 and 22.58%). The hemoglobin of the athletes increased significantly from a baseline of 12.69 g/dl to 16 and 16.66 g/dl in G1 and G2, respectively. Furthermore, the athletic performance was enhanced in vertical jump, long jump, sprinting velocity, half squats, and pushups, which reached 58.75 cm, 255 cm, 3.5 m/s, 218.75 counts, and 85 counts, respectively in G2. The healthy gut microbiome (probiotics) in parallel with increased iron bioavailability by mineral binding (whey bioactive peptides), influenced iron status and can represent a healthy practice to improve athletic anemia and performance. On the other hand, urinary albumin exceeded the border of reference range (<30 mg/g) and reached 38.25 and 44.13 mg/g in G1 and G2, while urine pH was in the normal range (4.5–8). Increased urinary albumin might be due to high rates of protein metabolism that follow high protein intake. This study provided preliminary information on metabolic responses to high protein concentrated yogurt intake in athletes who engaged in daily exercise. Further studies are needed to determine the recommended intensity of 10 and 20% Pro-WPI product consumption to achieve its benefits and avoid implications on kidney function.
Survival Outcomes of Patients With Epidermal Growth Factor Receptor Mutations in Non-Small Cell Lung Cancer With Leptomeningeal Metastasis
Authors: Li Ning , Bian Zhimin , Cong Minghua , Liu Yutao
Published: January 2022

Background

Leptomeningeal metastasis (LM) is a commonly observed complication in patients with epidermal growth factor receptor (EGFR)-mutated non-small cell lung cancer (NSCLC). This study aimed to investigate the gene mutations, treatment strategies, and clinical outcomes in patients with LM.

Methods

We retrospectively analyzed the clinical and survival outcomes of 53 patients with EGFR-mutated NSCLC with LM.

Results

The median overall survival after LM diagnosis was 13.0 months, ranging from 0.5 to 42.0 months (95% CI = 9.067–16.933), with 64.2% maturity. Patients who received osimertinib after developing LM (n = 35) had a significantly higher rate of LM disease control (p = 0.008) and significantly longer overall survival (15.0 versus 6.0 months; hazard ratio (HR), 2.4292; 95% CI, 1.234–4.779; p = 0.045) than those who received previous generations of EGFR tyrosine kinase inhibitors (TKIs) or other localized therapies (n = 6). Logistic regression analysis showed that LM disease control status was a positive predictive factor for overall survival after developing LM (p < 0.001, odds ratio = 10.797, 95% CI = 4.102–28.419).

Conclusions

Our study provides real-world clinical evidence that patients with EGFR-mutated NSCLC diagnosed with LM who developed LM had better clinical outcomes with osimertinib therapy. Our findings also suggest that LM disease control is the most effective strategy to prolong the overall survival outcomes of these patients.
Pembrolizumab Plus Chemotherapy in Metastatic Thymic Carcinoma: A Case Report
Authors: Thomas Quentin Dominique , Basse Clémence , Luporsi Marie , Girard Nicolas
Published: January 2022

Metastatic thymic carcinomas have a poor prognosis. Pembrolizumab, an anti-PD-1 antibody, has recently been evaluated for patients with metastatic thymic carcinomas progressing after at least one line of platinum-based chemotherapy. The antitumor activity of immunotherapy appears to be promising for these patients and pembrolizumab in monotherapy is actually a treatment option in second metastatic line. To the best of our knowledge, we report the first case of a patient treated for metastatic thymic adenocarcinoma with a combination of chemotherapy–immunotherapy. The patient is a 46-year-old man with metastatic thymic adenocarcinoma treated in third metastatic line with a combination of pembrolizumab plus platinum-based chemotherapy with a very good metabolic tumor response. He had a progression-free survival of 7.9 months and did not experience any severe side effects related to pembrolizumab. The association of immunotherapy and chemotherapy, as in non-small cell and small cell lung cancers, could be of interest for future therapeutic trials evaluating the survival of patients with metastatic thymic carcinoma.
Physical Activity and Executive Function in Children With ADHD: The Mediating Role of Sleep
Authors: Liang Xiao , Li Ru , Wong Stephen H. S. , Sum Raymond K. W. , Wang Peng , Yang Binrang , Sit Cindy H. P.
Published: January 2022

This study examined the mediating role of sleep in the relationship between physical activity and executive function in children with attention deficit hyperactivity disorder (ADHD). Fifty-six children with ADHD were recruited from Shenzhen Children's Hospital. Participants wore an accelerometer for seven consecutive days to measure physical activity and sleep quality. Activity counts were analyzed to measure moderate-to-vigorous physical activity (MVPA). Four sleep parameters, including sleep latency (SL), sleep efficiency, total sleep time, and wake after sleep onset were recorded from the actigraph. Three core executive functions, inhibitory control; working memory (WM); and cognitive flexibility (CF), were assessed from computer-based tasks: the flanker task, and the Tower of London and Trail Making Tests, respectively. The regression results showed that MVPA was negatively associated with SL (−0.169; 95%CI [−0.244, −0.112]). WM (total scores) was positively related to MVPA (0.028, 95%CI [0.008, 0.048]), but negatively related to SL (−0.105, 95%CI [−0.167, −0.030]). CF (part B errors) was negatively associated with MVPA (−0.031, 95%CI [−0.055, −0.005]) and positively correlated with SL (0.184, 95%CI [0.092, −0.260]). The indirect effect of SL was found for MVPA and WM (0.018, 95%CI [0.015, 0.034]), supporting the indirect partial mediation. Similarly, the indirect effect of SL was found between MVPA and CF (−0.031, 95%CI [−0.060, −0.012]), supporting the indirect partial mediation. The mediating role of SL in children with ADHD suggests that the intensity of physical activity plays a key role in linking sleep quality and executive function in this group.