bluuebunny/crossref_metadata_2025 huggingface dataset:
PENETAPAN KADAR PEWARNA TARTRAZIN PADA MIE INSTAN DENGAN METODE SPEKTROFOTOMETRI UV–VIS
Authors: Wulandari Yuyun
Published: March 2022

Mie instan merupakan salah satu contoh makanan instan atau siap saji. Didalam mie instan terdapat zat pewarna sintetis. Warna merupakan daya tarik terbesar untuk menikmati makanan setelah aroma. Namun penggunaan pewarna sintetis harus dilakukan sesuai dengan peraturan yang berlaku karena dapat merugikan kesehatan. Oleh karena itu perlu dilakukan monitoring pewarna sintetis pada makanan. Metode analisis yang digunakan adalah Spektrofotometri Uv-Vis. Pewarna sintetis yang terkandung dalam sampel tersebut adalah pewarna yang memungkinkan penggunaannya untuk makanan oleh PERMENKES RI seperti Tartrazin. Telah dianalisis bahwa pewarna tartrazin memiliki panjang gelombang maksimum 430 nm dengan kadar Sampel A 11,3 mg/Kg, Sampel B 7,5 mg/Kg, Sampel C 12,3 mg/Kg, Sampel D 6,3 mg/Kg, Sampel E 8,4 mg/Kg, Sampel F 5,4 mg/Kg, Sampel G 8,8 mg/Kg. Berdasarkan BPOM RI Nomor 37 Tahun 2013 tentang Batas Maksimum Penggunaan BTP Pewarna ditetapkan bahwa pewarna tartrazin diizinkan dalam kategori pangan pada pasta dan mie denganbatas maksimum 70 mg/Kg. Kata kunci: Pewarna Sintetis, Tartrazin, Spektrofotometri Uv-Vis
PENETAPAN KADAR PROTEIN PADA JERAMI CEMPEDAK (Artocarpuschempeden) DAN JERAMI NANGKA (Artocarpusheterpophyllus L.) DENGAN METODEKJELDAHL
Authors: Primadiamanti Annisa , Ulfa Ade Maria , Amalia Fitri
Published: March 2022

Protein merupakan suatu zat makanan yang amat penting bagi tubuh yang berfungsi sebagai zat pembangun, pengatur, dan sebagai sumber energi. Salah satu sumber protein terdapat pada jerami buah nangka dan jerami buah cempedak. Jerami nangka dan jerami cempedak merupakan limbah buah nangka dan buah cempedak yang terbatas sebagai pakan ternak atau dibuang, dan juga berpotensi sebagai bahan baku berbagai hasil olahan pangan atau industri pangan seperti selai. Tujuan penelitian ini untuk mengetahui kadar protein pada jerami nangka dan jerami cempedak dengan metode Kjeldahl. Metode Kjeldahl merupakan metode yang cukup spesifik untuk menentukan jumlah protein dengan penentuan kandungan nitrogen yang ada didalam makanan. Sampel diambil dari Pasar Bambu Kuning Bandar Lampung. Hasil pengujian kualitatif protein pada jerami nangka dan jerami cempedak secara uji biuret yaitu terbentuknya warna ungu pada sampel yang menunjukan sampel positif mengandung protein, dan pada pengujian kuantitatif dengan metode Kjeldahl diperoleh kadar protein pada jerami cempedak sebesar 5,6% ± 0,27 dan pada jerami nangka sebesar 2,71% ± 1,22.Kata kunci : jerami nangka, jerami cempedak, protein, Kjeldahl
EFICÁCIA DO MÉTODO PILATES EM PESSOAS SAUDÁVEIS: UMA REVISÃO NARRATIVA
Authors: Bregalda Juliane , Sudatti Delevatti Rodrigo
Published: March 2022

Esta pesquisa possui o objetivo de verificar na literatura, como vem sendo atestada a eficácia do método Pilates e sua influência em indivíduos saudáveis. Trata-se de uma revisão narrativa. Os artigos foram selecionados em maio de 2021, nas bases de dados PubMed e Lilacs, sendo utilizada a palavra-chave Pilates e o filtro para ensaios clínicos. Foram incluídos artigos que cumpriram os seguintes critérios: estudos clínicos randomizados, controlados, com indivíduos saudáveis, com grupo de intervenção em Pilates, nas línguas portuguesa e inglesa, com acesso gratuito e publicados nos últimos 5 anos, exceto artigos de revisão de literatura. Dos 234 artigos encontrados na busca, 4 artigos foram selecionados. A extração dos dados foi feita de forma padronizada com os seguintes dados: título, referências bibliográficas, método (número de participantes, características da amostra, grupos em que a amostra foi randomizada, frequência semanal, duração da intervenção, duração da sessão, exercícios e modo de progressão utilizados na intervenção), objetivo do estudo e principais resultados. Observou-se que a intervenção do método Pilates, de maneira geral, foi capaz de gerar melhorias na maior parte dos desfechos pesquisados em indivíduos saudáveis, sendo eles: equilíbrio, força, flexibilidade, qualidade de vida, postura e desempenho na corrida.
Evaluating the Low-temperature Properties of Asphalt Binders Extracted from Mixtures Containing Recycled Materials
Authors: Deef-Allah Eslam , Abdelrahman Magdy
Published: March 2022

The use of recycled materials − such as reclaimed asphalt pavement (RAP) and recycled asphalt shingles (RAS) − in asphalt mixtures reduces natural resources demands and decreases materials dumped in landfills. The aged binders included in the recycled materials alter binders’ low-temperature properties included in asphalt mixtures. Therefore, asphalt binders were extracted from asphalt mixtures collected from the field as cores. Due to the limited amount of extracted asphalt binders (EABs), a dynamic shear rheometer was used to examine the low-temperature properties [e.g., true temperature (Tt), continuous temperature (Tc), and delta continuous temperature (ΔTc)]. Using recycled materials in asphalt mixtures increased EABs’ low temperatures, Tt and Tc , and decreased EABs’ ΔTc values when compared to EABs from mixtures without recycled materials. Using RAS in asphalt mixtures degraded the low-temperature properties of EABs, Tt and Tc increased and ΔTc decreased, when compared to EABs from mixtures containing RAP. Increasing the asphalt binder replacement (ABR) percentages by recycled materials increased Tc and decreased ΔTc . The flow activation energy (Ea) was related to the Tc and ΔTc values, and very strong relationships were observed between Ea and Tc and Ea and ΔTc . The researchers modelled two low-temperature prediction models to predict Tc and ΔTc depending on the grade of the virgin asphalt binder, ABR types and percentages, and asphalt mixtures ages.
Indicadores técnicos de desempenho para comparação de equipes de sucesso e não sucesso na Bundesliga
Authors: Domingos Luis Felipe , Cambre Añon Iago
Published: March 2022

Objetivo: Este estudo propôs comparar e identificar variáveis técnicas capazes de diferenciar as equipes de melhor desempenho das demais equipes que disputaram a Bundesliga nas últimas cinco temporadas.

Método: Foram coletados dados de nove variáveis técnicas de 1530 jogos da Bundesliga entre as temporadas 2014-15 e 2018-19 através do site público “whoscored.com”. As equipes presentes na tabela da Bundesliga nas últimas cinco temporadas foram divididas em grupos de três. Após a coleta, utilizou-se o teste de Shapiro-Wilk para a determinação da distribuição dessas variáveis. Para os dados cuja distribuição é normal foi utilizado o teste T de amostras independente e para os dados cuja distribuição não foi normal, utilizou-se o teste de Mann-Whitney.

Resultado: Os resultados mostraram que as equipes do Grupo 1 apresentaram melhores índices nas variáveis chute de dentro da área, chute de fora da área, passe para setor de meio, passe para setor de ataque e desarme pelo tempo de posse de bola adversária.

Conclusão: O estudo se mostrou satisfatório pois cumpriu com seu objetivo ao identificar variáveis técnicas que discriminam equipes vencedoras das outras equipes, além de proporcionar aos treinadores informações para definir objetivos para equipe e jogadores nas sessões de treinamento.
Acute effects of swimming aerobic exercise on contractility and intracellular calcium handling in isolated right ventricular cardiomyocytes
Authors: Santos Jean Carlos Loura dos , Claudio Erick Roberto Gonçalves , Lunz Wellington , Morra Elis Aguiar , Silva Vitor Loureiro da , Cordeiro Jóctan Pimentel , Bocalini Danilo Sales , Lima Leopoldo Ana Paula , Leopoldo Andre S
Published: March 2022

Aims: The acute adjustments on the contractile function and the intracellular calcium (Ca2+) handling in the cardiomyocytes of the right ventricle (RV) after an acute aerobic exercise session are not known. Our aim was to investigate the acute responses of the contractile function and the intracellular Ca2+ handling in isolated RV cardiomyocytes after a swimming exercise session.

Methods: Ten-week-old female Wistar rats were randomly allocated into two groups: control (C; n = 5) and exercise (Ex; n = 7). It was performed a swimming exercise session for 30 min. with an overload of 4% relative to the body weight attached to the tail. The rats were sacrificed after the exercise session for the analysis of the RV contractile function parameters and the intracellular Ca2+ handling by the isolated cardiomyocyte technique.

Results: Body and heart weights, as well as sarcomere length were similar between the groups. Also, it was not observed differences between the groups for RV cardiomyocyte contractile parameters. However, the systolic and diastolic intracellular Ca2+ concentration was significantly lower in the Ex group compared to C with maintenance of Ca2+ amplitude.

Conclusion: An acute swimming aerobic exercise session promotes cardiomyocyte contractility maintenance even with systolic and diastolic intracellular Ca2+ concentration reduced in the RV cardiomyocytes, reflecting an improvement in the intracellular Ca2+ handling.
Portugal na Era dos Homens Fortes: Democracia e Autoritarismo em Tempos de Covid
Authors: Riley Maria
Published: March 2022

Lima, B. (2020). Portugal na Era dos Homens Fortes: Democracia e Autoritarismo em Tempos de Covid. Lisboa: Tinta Da China.
ARCHITECTURAL NAMES DERIVING FROM THE VERB *STATI/ STAVITI IN THE LANGUAGES OF THE BALKANSLAVIC CONTINUUM
Authors:   Kyiv National Linguistic University Kyiv, Ukraine, PASHKOVA N.I.
Published: March 2022

The article examines the semantics and internal form of architectural names motivated by the common Slavic verb *stati / staviti, wich has the Indo-European origin, in the Ukrainian language and its dialects, as well as their parallels in the languages of the Balkan-Slavic continuum. We call the Balkan-Slavic lingual continuum the integrate lingual space of the Balkans, Carpats and Slavia, which includes the languages of the Balkan Peninsula, as well as all Slavic languages, not only those that are part of the Balkan language union. In particular, ukr. stajnja, stan, stąja, zastajka, stajenka, styna, stodola, pol. stania, stań, stajnia, stajna, stąj, stąja, stodoła, chech stání, stájňa, stáj, stájе, chech and slovac stajna, staj, staja, stodola, slovac stajеń, stajňa, stajńja, chech, slovac, slovenian stavba, slovenian stajеń, stajňa, stajńja, stavisko, štala, blg. stuna, blg. and mak. staja, blg., srb., slvc., croat, chech stan, srb., vojev. staj, staja, stajnica, croat., montenegr. staja, sln. staj, stájа, stajna, stajnica, blg., srb. stan, mak. stanovi, rom. stînă, gr. στάνη, hung. esztena, and their phonetical and morphological variants have been studied. Based on the semantic and word-formation analysis of the lexical material, conclusions are drawn that the studied words are formed on the basis of common European nominative models for names of housing and livestock premises, motivated by verbs with the meaning ‛put’. It is determined that the nominative models and semantics of names, which reflects the different purpose of buildings, are due to a certain shade of meaning of the verbmotivator. The considered architectural names are formed according to two productive word-forming models ‛to build’ — ‛building’ and ‛to put’ — ‛reservoir, room’. The names of premises for livestock are formed according to the final nominative model, which is based on the purpose, functions and meaning of the verb ‛to place’ (stable ‛place where cattle are placed or where they stand’), and the names of buildings for people are formed according to causal nominative model, which is based on the meaning of the verb ‛to put, to build’ (building). Separate groups are East, South and West Slavic formations and mutual borrowings between Slavic languages and languages of the Carpathian-Balkan continuum.
SIMILARITY, COHESION, DISTANCING AND FRIENDSHIP QUALITY AMONG ITALIAN AND BOLIVIAN BOYS AND GIRLS: A CROSS NATIONAL COMPARISON
Authors: Rabaglietti Emanuela , Vacirca Maria Fernanda , Zucchetti Giulia , Camacho Carmen , Ciairano Silvia
Published: March 2022

This study aimed to contribute at exploring friendship in children from two different countries, Italy and Bolivia, focusing on the association between specific characteristics of children’s friendships, such as similarity, cohesion and distance with friends as represented by the children through drawing, and the perception of their friendship quality. Participants were 100 children (55 Italians and 45 Bolivians) attending primary schools. The two subgroups are balanced for gender and age [Italian girls: 56%, N=31, Bolivian girls 51%, N=23; M age Italian children=8.09 (SD = .74) and Bolivian children M age=7.91 (SD = .90)]. We used children’s drawings and self-reported measures to investigate the dyadic characteristics and the quality of their friendship. Results showed that Italian children drawn themselves and their best friend more similar than Bolivian children [F (1, 94) = 6.65; p<.002]. There are more conflicts in the friendships of Bolivian children than in the Italian ones [F (1, 98) = 7.87; p<. 006]. There is a moderation effect of children's nationality on the association between the pictorial friendship features and friendship quality. These preliminary findings highlighted the importance of investigating children's perceptions of friendship, which is a complex and multidimensional phenomenon, taking into account the social and cultural differences.
Key words: cohesion, cross-national differences, distancing, friendship quality, similarity.
ADOLESCENTS COMMUNICATION, CONFLICT STYLES AND CONFLICT RESOLUTIONS WITH PARENTS, PEERS AND ROMANTIC PARTNERS: SIMILARITY, DIFFERENCES AND RELATIONS WITH SATISFACTION
Authors: Roggero Antonella , Rabaglietti Emanuela , Settanni Michele , Ciairano Silvia
Published: March 2022

Communication and conflict styles and resolutions are key issues for understanding the dynamics underlying every social relationships. However they are usually investigated separately in different social contexts. In the current study, the similarities and differences of the adolescents’ communication, conflict styles, conflict resolution and satisfaction with parents, best friends and boy/girlfriend were explored.
One hundred and three Italian adolescents, aged 15 to 20years, were administered a version of the Parent-Adolescent Communication Scale (PACS; Barnes, & Olson, 1985). Exploratory principal component analyses, Cronbach’s alpha, Repeated Measures ANOVAs, correlation and hierarchical regression analyses were used to analyze the data.
The adolescents use similar levels of open and problem communication, compromise and aggression (conflict styles), intimacy and frustration (conflict resolutions) with father, mother, friends and romantic partners and they are similarly satisfied with their partners. However open communication, compromise and intimacy are more frequent with friends and romantic partners than with the parents.
Besides, the strongest predictor of satisfaction is always open communication. However, with the family and the boy/girlfriend the resolution of the conflict is also important, while with the friends the way to manage the conflict is relevant too.
Key words: adolescent social partners, communication, conflict, satisfaction.
PSYCHO-HYGIENIC СOMPETENCE OF SPECIALISTS IN INTERNET- ENVIRONMENT USE
Authors: Boltivets Sergiy
Published: March 2022

The awareness by the "man-man" professions specialists of their own psycho-hygienic skills and competence content, which are formed in the process of professionalization, is determined by conceptual ideas. They are developed within a certain people-study profession and cover use, interaction and reflection of a personality in the Internet environment.
We have introduced into scientific use the category of mental health quality which represents the individual property of personality to ensure his/her own procedural integrity, that is adequate to internal nature, in cooperation with own selfness and environment. This category certainly includes the continuingly improving means of both learning the world and self-implementation in it including the Internet-environment.
RATES OF INFORMATION AWARENESS ON AGGRESSIVE DRIVING AS THE INDICATORS OF THE EXISTING PROBLEMS IN THE SECTOR OF SPECIAL EDUCATION
Authors: Jenenkova Oksana
Published: March 2022

In the road traffic field the biggest danger is caused by a phenomenon of „aggressive driving”. The topicality of the problematic issue is noted by such organizations like the WHO and the UNO; as a result of this the term “aggressive driving” was introduced in Latvia on the legislative level. In the territory of Latvia a multi-stage study was performed; its aim was to study ideas of drivers on aggressive driving. During the study the following methods were used: method of associations, method of unfinished sentence, structured, partially structured and non-structured interview, personal differential method, socially demographic questionnaire and Aggressive Driving Questionnaire (Jenenkova, 2009). In all, 2160 drivers representing all regions of Latvia were questioned.
As a result of the study one of the components of ideas on aggressive driving was found out – information awareness, which is considered in this article. Information awareness is represented by the following component parts: self- evaluation of terminology knowledge (expected level), level of term understanding (expected level), term knowledge (real level), level of term understanding (real level), level of agreement with the formulation, information sources. During the comparative analysis of drivers’ group and inspectors’ group of the road traffic not only their differences by these components were discovered, but also the current problems in relation to the terminology knowledge and understanding were found out.
Key words: aggressive driving, dangerous driving, drivers and inspectors, information awareness, problems in terminology, road traffic.
SCHOOL MOTIVATION AND ACHIEVEMENT OF STUDENTS IN SECONDARY EDUCATION
Authors: Morales Rodríguez Francisco Manuel
Published: March 2022

This paper aims to analyse the importance of intrinsic motivation factors as determinants of academic achievement in English as a Foreign Language (EFL). The general aim of the present study has been to analyse the existing relationship between students’ academic motivation and performance. This paper explores the importance of motivational variables for school achievement. In particular, the present study suggests that students with a higher intrinsic motivation to learn English will achieve better grades in this subject. Participants were 542 students (male and female) of Secondary Education. Results show that there is a correlation between academic goals and academic results; in particular an association exists between learning goals (intrinsic motivation) and a high academic achievement. With regards to the importance of intrinsic motivational factors, this study concludes that these factors are determinants of academic achievement in English as a Foreign Language (EFL). Therefore, results are useful in view of improving the process of teaching/learning EFL. The relevant conclusion reached here point to the academic goals as being indicator of and decisive factor for academic achievement.
Key words: academic achievement, academic goals, academic motivation, English as a foreign language.
UNDERSTANDING SELF-HARM IN YOUNG PEOPLE: AN EMOTIONAL UNBALANCE IN NEED FOR INTERVENTION
Authors: Reis Marta , Gaspar de Matos Margarida , Ramiro Lúcia , Figueira Isa
Published: March 2022

Self-harm behavior refers to the intentional self-destruction or alteration of body tissue without deliberate suicidal intent. Studies claimed self-harm as being associated to obsessive-compulsive characteristics and eating disorders, emotion-regulation functions when interpersonal distress occurs and physiological reactivity during a distressing task in association to social problem-solving deficits. Three national studies referring to young people will be presented. The main study’s aim was to understand frequency of self-harm in Portuguese students and understanding the associations between self-harm and risk behaviors. A convenience sample of 206 was collected among high school and university students. The instrument used was a self-completed questionnaire: “Teens Self-Destructive Scale”. A minority of self-harmers was found. Results suggested associations between self-harm, eating disorders and difficulties regarding self-regulation of emotions and that self-harm can be considered a non-adaptive and not successful personal strategy to manage emotions. Findings from the other two national studies will be referred. All together, these findings demonstrate the relevance of studies in this domain and helped producing guidelines in terms of therapeutic interventions. During the last decades, young people became more literate regarding health information and more aware of risk behaviors, but did not improve their literacy about emotions and how to deal with them. Are we in need for a "positive" approach to mental health promotion in young people?
Key words: protective factors, risk factors, self-harm, students.
THE EFFECTS OF A SOCIAL-EMOTIONAL LEARNING PROGRAM ON THE THINKING PATTERN OF A GROUP OF UNIVERSITY STUDENTS
Authors: Vaida Sebastian Raul
Published: March 2022

Social-Emotional Learning (SEL) is one of the best approaches to teaching students the skills needed to better adapt to life and develop on a personal and professional level. Your-SELF is an abbreviation for “Your Social Emotional Learning Facilitator” and represents a social-emotional learning program based on the Rational Emotive Behavior Theory (REBT). It was implemented and tested as a personal development program for university students, for an entire academic year, with 3-4 hours weekly trainings. It is also a powerful and effective way to decrease the level of irrationality in the students' thinking style, along with developing some major life competencies (self-awareness, self-management, social awareness, relationship skills, decision making). In order to test this idea, an experimental design with repeated measures was used and the results clearly support the hypothesis that a SEL program based on the principles of REBT can lead to a significant decrease in the level of irrationality of the students in the experimental group, compared to the control one. Also, the four sub-scales of the instrument used to investigate the level of irrationality (ABS2) were analyzed and the same significant results were found on the level of low frustration tolerance, self-downing/global evaluation and awfulizing. The only exception was demandingness, and a rationale for this is offered in the discussion part of the article. The study shows that such a program can be beneficial and useful for the students involved and it is worth implementing on a larger scale.
Key words: competencies; rational and irrational thinking pattern; rational emotive behavior education; social-emotional learning; training; university students.
SOCIAL REPRESENTATIONS OF MODERN RUSSIANS ABOUT THE LONELY PERSON
Authors: Safonova Marina
Published: March 2022

In the article results of studying of social representations of Russians about the lonely person are considered. The loneliness in the modern world has ceased to be for a long time a problem of the separate person, for the specific reasons not managed to adapt for a society, to build emotional mutual relations satisfying it with associates. It has turned to a serious social problem. Efficiency of the psychological help to lonely people, constructions and realizations of programs on preventive maintenance of chronic loneliness is in many respects connected with the account of the developed social representations about loneliness and the lonely person. Difficulties in studying of a phenomenon of loneliness are somewhat connected with necessity to receive the information which mentions deep intimate layers of the person. On the one hand, work mechanisms of psychological protection. The respondent starts to have a feeling of alarm because of necessity to give answers about itself, with another – the tendency to social desirability is shown. For minimization of these displays in research the method of the projective composition was used. During the content-analysis of the text information received by a method of projective compositions, 13 semantic components have been allocated, each of which opens certain lines of an image of the lonely person. Psychological interpretation of the received data has allowed to describe some prototypes of the lonely person developing in representations of contemporaries. The urgency of the use of a category "prototype" is connected with specific features of a social reality: complexity, dynamism, variability, a polysemy of the signs used for a categorization. According to model of frequency of signs, the prototype reflects most often meeting signs, accordingly, the prototype is acquired through fixing of the repeating elements inherent in an object, or a situation. The analysis of representations of respondents about the lonely person allows to allocate some developing prototypes: "careerist", «a lonely wolf», "homebody", " the unsocial person".
Key words: loneliness, social representations about loneliness, a method of the projective composition, a prototype of the lonely person.
Glossing The Shepheardes Calender in Latin Translation
Authors: Mennis Katie
Published: March 2022

This article examines two Latin translations of The Shepheardes Calender by John Dove (1584) and Theodore Bathurst ( c.1602) respectively. It explores their versions of three aspects of Spenserian pastoral (all prominent in E.K.'s gloss): community and competition; allegory and allusion; register and rusticity. Throughout, it argues for the influence of translation theory on the translations and The Shepheardes Calender. It revises misinformation about the translations, demonstrating that Dove's translation influenced Bathurst's and that Bathurst's is collectively authored. It explores the way in which the translations ‘re-allegorize’ the Calender and reproduce Spenser's rustic style. While Bathurst's translation reveals an interest in Spenser's experience of patronage and poetic career, Dove attends to the poem's religious allegory, political significance, and linguistic agenda, ultimately using his translation to allude to the public disputations of Edmund Campion. Rather than ‘missing the point’ of Spenser's vernacular achievement, the translations extend the remit of Spenserian pastoral.
Drooling in Parkinson's disease and current treatment options
Authors: Abbasov F. A. , Yusupova M. M. , Bril E. V.
Published: March 2022

Drooling is a common non-motor symptom of Parkinson's disease, affecting about half of patients at different stages of the disease. Drooling can significantly reduce quality of life and be a source of aspiration. This article reviewsfeatures and prevalence of this symptom in Parkinson's disease, as well as its assessment and current treatment options, including botulinum toxin therapy.
Edukasi Risiko Finansial Teknologi pada Pondok Pesantren Rojaul Huda Darun Nasya Kabupaten Bandung Barat
Authors: Ismawati Linna , Asri Jatnika Dwi , Kianggi Kwik , Salsabila Tiara
Published: March 2022

Finansial Teknologi merupakan salah satu inovasi teknologi berbasis digital pada era revolusi industri 4.0 yang secara masif mengalami pertumbuhan sangat pesat. Perkembangan finansial teknologi diikuti dengan kemampuan masyarakat dalam mengadaptasi finansial teknologi dan digunakan secara masif dalam kehidupan sehari-hari. Kemudahan bertransaksi menggunakan aplikasi finansial teknologi mengubah gaya hidup masyarakat dari pemanfaatan teknologi konvensional ke teknologi digital. Disisi lain keterbatasan pengetahuan masyarakat tentang finansial teknologi mengakibatkan sebagian masyarakat mengalami risiko kerugian dalam pemanfaatan teknologi berbasis digital. Kurangnya pengetahuan menimbulkan berbagai permasalahan yang dihadapi terkait finansial teknologi di antaranya pola hidup yang semakin konsumtif, risiko pinjaman online yang mengalami gagal bayar, dan risiko kegagalan investasi. Berdasarkan hal tersebut maka tujuan program pengabdian dan pemberdayaan masyarakat ini adalah mengedukasi dan memberikan pendampingan kepada para santri Pondok Pesantren Rojaul Huda Darun Nasya agar memiliki pengetahuan yang cukup dalam memanfaatkan finansial teknologi dan meminimalisir risiko. Metode yang digunakan adalah (1) melakukan penjajakan kemitraan dan mendiskusikan topik pengabdian (2) melakukan pengamatan untuk menemukan masalah yang terjadi pada mitra; (3) memberikan penjelasan tentang kegiatan pengabdian kepada mitra; (4) melaksanakan kegiatan pengabdian di lokasi mitra. Luaran yang diperoleh dari pelaksanaan Program Pengabdian dan Pemberdayaan ini yaitu meningkatkan pengetahuan dan keterampilan dalam pemanfaatan finansial teknologi oleh generasi muda. Kesimpulan dari kegiatan ini adalah pentingnya edukasi kepada masyarakat dalam pemanfaatan finansial teknologi agar terhindar dari risiko kerugian.
Multi-Dimensional Spectral Single Molecule Localization Microscopy
Authors: Butler Corey , Saraceno G Ezequiel , Kechkar Adel , Bénac Nathan , Studer Vincent , Dupuis Julien P. , Groc Laurent , Galland Rémi , Sibarita Jean-Baptiste
Published: March 2022

Single molecule localization (SML) and tracking (SPT) techniques, such as (spt)PALM, (u/DNA)PAINT and quantum dot tracking, have given unprecedented insight into the nanoscale molecular organization and dynamics in living cells. They allow monitoring individual proteins with millisecond temporal resolution and high spatial resolution (<30 nm) by precisely localizing the point spread function (PSF) of individual emitters and tracking their position over time. While SPT methods have been extended to study the temporal dynamics and co-organization of multiple proteins, conventional experimental setups are restricted in the number of proteins they can probe simultaneously and usually have to tradeoff between the number of colors, the spatio-temporal resolution, and the field of view. Yet, localizing and tracking several proteins simultaneously at high spatial and temporal resolution within large field of views can provide important biological insights. By employing a dual-objective spectral imaging configuration compatible with live cell imaging combined with dedicated computation tools, we demonstrate simultaneous 3D single particle localization and tracking of multiple distinct species over large field of views to be feasible without compromising spatio-temporal resolution. The dispersive element introduced into the second optical path induces a spectrally dependent displacement, which we used to analytically separate up to five different fluorescent species of single emitters based on their emission spectra. We used commercially available microscope bodies aligned one on top of the other, offering biologists with a very ergonomic and flexible instrument covering a broad range of SMLM applications. Finally, we developed a powerful freely available software, called PALMTracer, which allows to quantitatively assess 3D + t + λ SMLM data. We illustrate the capacity of our approach by performing multi-color 3D DNA-PAINT of fixed samples, and demonstrate simultaneous tracking of multiple receptors in live fibroblast and neuron cultures.
Patient-Specific Cerebral Blood Flow Simulation Based on Commonly Available Clinical Datasets
Authors: Shen Yuanyuan , Wei Yanji , Bokkers Reinoud P. H. , Uyttenboogaart Maarten , Van Dijk J. Marc C.
Published: March 2022

Cerebral hemodynamics play an important role in the development of cerebrovascular diseases. In this work, we propose a numerical framework for modeling patient-specific cerebral blood flow, using commonly available clinical datasets. Our hemodynamic model was developed using Simscape Fluids library in Simulink, based on a block diagram language. Medical imaging data obtained from computerized tomography angiography (CTA) in 59 patients with aneurysmal subarachnoid hemorrhage was used to extract arterial geometry parameters. Flow information obtained from transcranial Doppler (TCD) measurement was employed to calibrate input parameters of the hemodynamic model. The results show that the proposed numerical model can reproduce blood flow in the circle of Willis (CoW) per patient per measurement set. The resistance at the distal end of each terminal branch was the predominant parameter for the flow distribution in the CoW. The proposed model may be a promising tool for assessing cerebral hemodynamics in patients with cerebrovascular disease.
Myc Supports Self-Renewal of Basal Cells in the Esophageal Epithelium
Authors: Hishida Tomoaki , Vazquez-Ferrer Eric , Hishida-Nozaki Yuriko , Takemoto Yuto , Hatanaka Fumiyuki , Yoshida Kei , Prieto Javier , Sahu Sanjeeb Kumar , Takahashi Yuta , Reddy Pradeep , O’Keefe David D. , Rodriguez Esteban Concepcion , Knoepfler Paul S. , Nuñez Delicado Estrella , Castells Antoni , Campistol Josep M. , Kato Ryuji , Nakagawa Hiroshi , Izpisua Belmonte Juan Carlos
Published: March 2022

It is widely believed that cellular senescence plays a critical role in both aging and cancer, and that senescence is a fundamental, permanent growth arrest that somatic cells cannot avoid. Here we show that Myc plays an important role in self-renewal of esophageal epithelial cells, contributing to their resistance to cellular senescence. Myc is homogeneously expressed in basal cells of the esophageal epithelium and Myc positively regulates their self-renewal by maintaining their undifferentiated state. Indeed, Myc knockout induced a loss of the undifferentiated state of esophageal epithelial cells resulting in cellular senescence while forced MYC expression promoted oncogenic cell proliferation. A superoxide scavenger counteracted Myc knockout-induced senescence, therefore suggesting that a mitochondrial superoxide takes part in inducing senescence. Taken together, these analyses reveal extremely low levels of cellular senescence and senescence-associated phenotypes in the esophageal epithelium, as well as a critical role for Myc in self-renewal of basal cells in this organ. This provides new avenues for studying and understanding the links between stemness and resistance to cellular senescence.
Hand Transplants, Daily Functioning, and the Human Capacity for Limb Regeneration
Authors: Fitzpatrick Susan M. , Brogan David , Grover Prateek
Published: March 2022

Unlike some of our invertebrate and vertebrate cousins with the capacity to regenerate limbs after traumatic loss, humans do not have the ability to regrow arms or legs lost to injury or disease. For the millions of people worldwide who have lost a limb after birth, the primary route to regaining function and minimizing future complications is via rehabilitation, prosthetic devices, assistive aids, health system robustness, and social safety net structures. The majority of limbs lost are lower limbs (legs), with diabetes and vascular disorders being significant causal contributors. Upper limbs (arms) are lost primarily because of trauma; digits and hands are the most common levels of loss. Even if much of the arm remains intact, upper limb amputation significantly impacts function, largely due to the loss of the hand. Human hands are marvels of evolution and permit a dexterity that enables a wide variety of function not readily replaced by devices. It is not surprising, therefore, for some individuals, dissatisfaction with available prosthetic options coupled with remarkable advances in hand surgery techniques is resulting in patients undertaking the rigors of a hand transplantation. While not “regeneration” in the sense of the enviable ability with which Axolotls can replace a lost limb, hand transplants do require significant regeneration of tissues and nerves. Regaining sophisticated hand functions also depends on “reconnecting” the donated hand with the areas of the human brain responsible for the sensory and motor processing required for complex actions. Human hand transplants are not without controversy and raise interesting challenges regarding the human regenerative capacity and the status of transplants for enabling function. More investigation is needed to address medical and ethical questions prior to expansion of hand transplants to a wider patient population.
Efficient Cholesterol Transport in Dendritic Cells Defines Optimal Exogenous Antigen Presentation and Toxoplasma gondii Proliferation
Authors: Croce Cristina , Garrido Facundo , Dinamarca Sofía , Santi-Rocca Julien , Marion Sabrina , Blanchard Nicolas , Mayorga Luis S. , Cebrian Ignacio
Published: March 2022

Dendritic cells are the most powerful antigen-presenting cells of the immune system. They present exogenous antigens associated with Major Histocompatibility Complex (MHC) Class II molecules through the classical pathway to stimulate CD4+ T cells, or with MHC-I to activate CD8+ T lymphocytes through the cross-presentation pathway. DCs represent one of the main cellular targets during infection by Toxoplasma gondii. This intracellular parasite incorporates essential nutrients, such as cholesterol, to grow and proliferate inside a highly specialized organelle, the parasitophorous vacuole (PV). While doing so, T. gondii modulates the host immune response through multiple interactions with proteins and lipids. Cholesterol is an important cellular component that regulates cellular physiology at the structural and functional levels. Although different studies describe the relevance of cholesterol transport for exogenous antigen presentation, the molecular mechanism underlying this process is not defined. Here, we focus our study on the inhibitor U18666A, a drug widely used to arrest multivesicular bodies biogenesis that interrupts cholesterol trafficking and changes the lipid composition of intracellular membranes. Upon bone marrow-derived DC (BMDC) treatment with U18666A, we evidenced a drastic disruption in the ability to present exogenous soluble and particulate antigens to CD4+ and CD8+ T cells. Strikingly, the presentation of T. gondii-associated antigens and parasite proliferation were hampered in treated cells. However, neither antigen uptake nor BMDC viability was significantly affected by the U18666A treatment. By contrast, this drug altered the transport of MHC-I and MHC-II molecules to the plasma membrane. Since U18666A impairs the formation of MVBs, we analyzed in T. gondii infected BMDCs the ESCRT machinery responsible for the generation of intraluminal vesicles. We observed that different MVBs markers, including ESCRT proteins, were recruited to the PV. Surprisingly, the main ESCRT-III component CHMP4b was massively recruited to the PV, and its expression level was upregulated upon BMDC infection by T. gondii. Finally, we demonstrated that BMDC treatment with U18666A interrupted cholesterol delivery and CHMP4b recruitment to the PV, which interfered with an efficient parasite replication. Altogether, our results highlight the importance of cholesterol trafficking and MVBs formation in DCs for optimal antigen presentation and T. gondii proliferation.
Design and Synthesis of Dipeptidomimetic Isocyanonaphthalene as Enhanced-Fluorescent Chemodosimeter for Sensing Mercury Ion and Living Cells
Authors: Wang Xiao-Juan , Li Gao-Wei , Cheng Yi-Peng , Sun Qiu-Ling , Hao Yuan-Qiang , Wang Chen-Hong , Liu Lan-Tao
Published: March 2022

A novel valine-based isocyanonaphthalene (NpI) was designed and synthesized by using an easy method and enabled the selective fluorescence detection of Hg2+. The chemodosimeter can display an immediate turn-on fluorescence response (500-fold) towards target metal ions upon the Hg2+-mediated conversion of isocyano to amino within NpI. Based on this specific reaction, the fluorescence-enhancement probe revealed a high sensitivity toward Hg2+ over other common metal ions and exhibited excellent aqueous solubility, good antijamming capability, high sensitivity (detection limit: 14.2 nM), and real-time detection. The response mechanism of NpI was supported by NMR spectroscopy, MS analysis and DFT theoretical calculation using various techniques. Moreover, a dipeptidomimetic NpI probe was successfully applied to visualize intracellular Hg2+ in living cells and monitor Hg2+ in real water samples with good recoveries and small relative standard deviations.
Reactive Oxygen Species-Responsive Nanococktail With Self-Amplificated Drug Release for Efficient Co-Delivery of Paclitaxel/Cucurbitacin B and Synergistic Treatment of Gastric Cancer
Authors: Pang Lijun , Zhang Lei , Zhou Hong , Cao Ling , Shao Yueqin , Li Tengyun
Published: March 2022

Application of drug combinations is a powerful strategy for the therapy of advanced gastric cancer. However, the clinical use of such combinations is greatly limited by the occurrence of severe systemic toxicity. Although polymeric-prodrug-based nanococktails can significantly reduce toxicity of drugs, they have been shown to have low intracellular drug release. To balance between efficacy and safety during application of polymeric-prodrug-based nanococktails, a reactive oxygen species (ROS)-responsive nanococktail (PCM) with self-amplification drug release was developed in this study. In summary, PCM micelles were co-assembled from ROS-sensitive cucurbitacin B (CuB) and paclitaxel (PTX) polymeric prodrug, which were fabricated by covalently grafting PTX and CuB to dextran via an ROS-sensitive linkage. To minimize the side effects of the PCM micelles, a polymeric-prodrug strategy was employed to prevent premature leakage. Once it entered cancer cells, PCM released CuB and PTX in response to ROS. Moreover, the released CuB further promoted ROS generation, which in turn enhanced drug release for better therapeutic effects. In vivo antitumor experiments showed that the PCM-treated group had lower tumor burden (tumor weight was reduced by 92%), but bodyweight loss was not significant. These results indicate that the developed polymeric prodrug, with a self-amplification drug release nanococktail strategy, can be an effective and safe strategy for cancer management.
Efficient Coagulation Removal of Fluoride Using Lanthanum Salts: Distribution and Chemical Behavior of Fluorine
Authors: Zhong Xiaocong , Chen Chen , Yan Kang , Zhong Shuiping , Wang Ruixiang , Xu Zhifeng
Published: March 2022

Abstract: La-loaded absorbents have been widely reported for fluoride removal due to the strong affinity of La3+ towards fluoride ion. Herein, chemical removal of fluoride from flue gas scrubbing wastewater using lanthanum salt is investigated. The retaining free F− concentration, phase composition and morphology of filtration residues, and the distribution of fluorine have been investigated using ion-selective electrode, analytical balance, scanning electron microscopy, and X-ray diffractor. The results show that at La/F molar ratio ≥1:3.05, the majority of fluorine exists as LaFx3−x complexes, leading to the failure of fluoride removal. At 1:3.20 ≤ La/F molar ratio ≤1:3.10, the formation of LaF3 is facilitated. However, co-existing LaFx3−x tends to absorb on the surface of LaF3 particles, leading to the formation of colloidal solution with large numbers of LaF3·LaFx3−x suspended solids. At an optimized La/F molar ratio of 1:3.10, a fluoride removal of 97.86% is obtained with retaining fluorine concentration of 6.42 mg L−1. Considering the existing of positively charged LaFx3−x and LaF3·LaFx3−x, coagulation removal of fluoride is proposed and investigated using lanthanum salts and negatively charged SiO2·nH2O colloidal particles, which is in-situ provided via Na2SiO3 hydrolysis at pH near 5.5. At a La/F molar ratio of 1:3.00 and Na2SiO3 dose of 0.50 g L−1, a fluoride removal of 99.25% is obtained with retaining fluorine concentration of 2.24 mg L−1. When Na2SiO3 dose increases to 1.00 g L−1, the retaining fluorine concentration could be further reduced to 0.80 mg L−1.
The Volcanic Hazards of Jan Mayen Island (North-Atlantic)
Authors: Gjerløw Eirik , Höskuldsson Ármann , Bartolini Stefania , Biass Sebastien , Mossoux Sophie , Gilbert Jennie , Pedersen Rolf Birger , Martí Joan
Published: March 2022

Hazard assessment of remote volcanic islands provides many challenges compared to other volcanoes and volcanic fields. Here we present the first systematic volcanic hazard assessment of Jan Mayen Island, a remote island located in the North-Atlantic Ocean and home to the northernmost active subaerial volcano in the world (Beerenberg Volcano), and we discuss some of the challenges and characteristics of performing a volcanic hazard assessment of a remote volcanic island. Jan Mayen has had at least five eruptions since its discovery at the start of the 17th century. Its Holocene volcanism is mainly characterized by eruptions with styles ranging from Hawaiian to Strombolian, but also by lava domes and Surtseyan eruptions. Based on field data, remote images, topographic data, past data, and computer simulations, our study evaluates the spatial probability of new vents opening, estimates eruption recurrence rates, simulates various eruption scenarios, and produces hazard maps for the different scenarios. This work shows where the hazards of ash fall, and lava flows are more likely to affect the built infrastructure on Jan Mayen Island. This hazard assessment will assist emergency planning and the determination of future land use on the island.
Construction Method Optimization for Transfer Section Between Cross Passage and Main Tunnel of Metro Station
Authors: Zhou P. Y. , Wang J. B. , Song Z. P. , Cao Z. L. , Pei Z. M.
Published: March 2022

The transfer section between the cross passage and the main tunnel is the part that needs to be paid attention to during the underground excavation construction of the subway. Due to complex stress, the collapse of the horsehead gate and excessive surface settlement often occur. In order to determine the construction scheme of the transfer section between the cross passage and the main tunnel of Guanshui Road Station of Metro Line two in Guiyang, China, the numerical simulation method was used to analyze the “double-holes interval pillar method,” “sector expansion method,” and “gate climbing method,”, respectively. The mechanical response of the surrounding rock and supporting structure under each method was compared. The comparisons showed that the surface settlement, the displacement of the cave, and the plastic zone caused by the double-holes interval pillar method were the smallest, and the method can reduce the construction risk, shorten the construction period, and reduce the project cost. Therefore, it was recommended to use the double-holes interval pillar method to construct the transfer section. The comparison between the measured data and the numerical simulation results of the double-holes interval pillar method showed that the numerical simulation results were smaller than the measured data at each point, and the surface settlement and horizontal displacement in the tunnel both met the safety control standard.