bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-GPU-Parallel and Tile-Based Kernel Density Estimation for Large-Scale Spatial Point Pattern Analysis
Authors: Zhang Guiming , Xu Jin
Published: January 2023

Kernel density estimation (KDE) is a commonly used method for spatial point pattern analysis, but it is computationally demanding when analyzing large datasets. GPU-based parallel computing has been adopted to address such computational challenges. The existing GPU-parallel KDE method, however, utilizes only one GPU for parallel computing. Additionally, it assumes that the input data can be held in GPU memory all at once for computation, which is unrealistic when conducting KDE analysis over large geographic areas at high resolution. This study develops a multi-GPU-parallel and tile-based KDE algorithm to overcome these limitations. It exploits multiple GPUs to speedup complex KDE computation by distributing computation across GPUs, and approaches density estimation with a tile-based strategy to bypass the memory bottleneck. Experiment results show that the parallel KDE algorithm running on multiple GPUs achieves significant speedups over running on a single GPU, and higher speedups are achieved on KDE tasks of a larger problem size. The tile-based strategy renders it feasible to estimate high-resolution density surfaces over large areas even on GPUs with only limited memory. Multi-GPU parallel computing and tile-based density estimation, while incurring very little computational overhead, effectively enable conducting KDE for large-scale spatial point pattern analysis on geospatial big data.
Spatial Application of Southern U.S. Pine Water Yield for Prioritizing Forest Management Activities
Authors: Vernon Jordan , St. Peter Joseph , Crandall Christy , Awowale Olufunke E. , Medley Paul , Drake Jason , Ibeanusi Victor
Published: January 2023

Forest management depends on forest condition data and the ability to quantify the impacts of management activities to make informed decisions. Spatially quantifying water yield (WY) from forests across large landscapes enables managers to consider potential WY changes when designing forest management plans. Current forest water yield datasets are either spatially coarse or too restricted to specific sites with in situ monitoring to support some project-level forest management decisions. In this study, we spatially apply a stand-level southern pine WY model over a forested landscape in the Florida panhandle. We informed the WY model with pine leaf area index inputs created from lidar remote sensing and field data, a spatial and temporal aridity index from PRISM and MODIS data, and a custom depth to groundwater dataset. Baseline WY conditions for the study area were created using the Esri and Python tools we developed to automate the WY workflow. Several timber thinning scenarios were then used to quantify water yield increases from forest management activities. The results of this methodology are detailed (10 m spatial resolution) forest WY raster datasets that are currently being integrated with other spatial datasets to inform forest management decisions.
TAT Nanobody Exerts Antiviral Effect against PRRSV In Vitro by Targeting Viral Nucleocapsid Protein
Authors: Ren Jiahui , Duan Hong , Dong Haoxin , Wu Shuya , Du Yongkun , Zhang Gaiping , Zhang Angke
Published: January 2023

Porcine reproductive and respiratory syndrome (PRRS) is caused by the PRRS virus (PRRSV), which has brought huge economic losses to the pork industry worldwide since its first discovery in the late 1980s in North America. To date, there are no effective commercial vaccines or therapeutic drugs available for controlling the spread of PRRSV. Due to their unique advantages of high affinity and high specificity, nanobodies (Nbs) have received increasing attention in the process of disease diagnosis and treatment. Trans-activator transcription (TAT) can serve as a vector to carry specific proteins into cells by passing through cell membranes. In our previous study, a specific Nb against the PRRSV nucleocapsid (N) protein was screened using phage display technology. For this study, we developed a novel recombinant protein constituting a TAT-conjugated Nb, which we call TAT-Nb1. The target cell entry efficiency of TAT-Nb1 and its effect on PRRSV infection and replication were then investigated. Our results indicate that TAT delivered Nb1 into Marc-145 cells and porcine alveolar macrophages (PAMs) in a dose- and time-dependent manner. Furthermore, TAT-Nb1 dose-dependently suppressed PRRSV infection and replication, where this antiviral effect was independent of PRRSV strain. Co-immunoprecipitation results revealed that Nb1 efficiently interacted with the N protein of PRRSV. Taken together, the presented results suggest that TAT-Nb1 can effectively suppress PRRSV replication, and it may be considered as a new anti-PRRSV candidate drug.
Sly-miR398 Participates in Cadmium Stress Acclimation by Regulating Antioxidant System and Cadmium Transport in Tomato (Solanum lycopersicum)
Authors: Yan Guochao , Hua Yuchen , Jin Han , Huang Qingying , Zhou Guanfeng , Xu Yunmin , He Yong , Zhu Zhujun
Published: January 2023

Cadmium (Cd) pollution is one of the major threats in agricultural production, and can cause oxidative damage and growth limitation in plants. MicroRNA398 (miR398) is involved in plant resistance to different stresses, and the post-transcriptional regulation of miR398 on CSDs plays a key role. Here, we report that miR398 was down-regulated in tomato in response to Cd stress. Simultaneously, CSD1 and SOD were up-regulated, with CSD2 unchanged, suggesting CSD1 is involved in miR398-induced regulation under Cd stress. In addition, the role of miR398 in Cd tolerance in tomato was evaluated using a transgenic line overexpressing MIR398 (miR398#OE) in which the down-expression of miR398 was disrupted. The results showed that Cd stress induced more significant growth inhibition, oxidative damage, and antioxidant enzymes disorder in miR398#OE than that in wild type (WT). Moreover, higher Cd concentration in the shoot and xylem sap, and net Cd influx rate, were observed in miR398#OE, which could be due to the increased Cd uptake genes (IRT1, IRT2, and NRAMP2) and decreased Cd compartmentalization gene HMA3. Overall, our results indicate that down-regulated miR398 plays a protective role in tomato against Cd stress by modulating the activity of antioxidant enzymes and Cd uptake and translocation.
Increased Levels of the Parkinson’s Disease-Associated Gene ITPKB Correlate with Higher Expression Levels of α-Synuclein, Independent of Mutation Status
Authors: Di Leva Francesca , Filosi Michele , Oyston Lisa , Silvestri Erica , Picard Anne , Lavdas Alexandros A. , Lobbestael Evy , Baekelandt Veerle , Neely G. Gregory , Pramstaller Peter P. , Hicks Andrew A. , Corti Corrado
Published: January 2023

Autosomal dominant mutations in the gene encoding α-synuclein (SNCA) were the first to be linked with hereditary Parkinson’s disease (PD). Duplication and triplication of SNCA has been observed in PD patients, together with mutations at the N-terminal of the protein, among which A30P and A53T influence the formation of fibrils. By overexpressing human α-synuclein in the neuronal system of Drosophila, we functionally validated the ability of IP3K2, an ortholog of the GWAS identified risk gene, Inositol-trisphosphate 3-kinase B (ITPKB), to modulate α-synuclein toxicity in vivo. ITPKB mRNA and protein levels were also increased in SK-N-SH cells overexpressing wild-type α-synuclein, A53T or A30P mutants. Kinase overexpression was detected in the cytoplasmatic and in the nuclear compartments in all α-synuclein cell types. By quantifying mRNAs in the cortex of PD patients, we observed higher levels of ITPKB mRNA when SNCA was expressed more (p < 0.05), compared to controls. A positive correlation was also observed between SNCA and ITPKB expression in the cortex of patients, which was not seen in the controls. We replicated this observation in a public dataset. Our data, generated in SK-N-SH cells and in cortex from PD patients, show that the expression of α-synuclein and ITPKB is correlated in pathological situations.
Exploration of the Main Antibiofilm Substance of Lactobacillus plantarum ATCC 14917 and Its Effect against Streptococcus mutans
Authors: Liang Jingheng , Zhou Yan , Tang Guihua , Wu Ruixue , Lin Huancai
Published: January 2023

Dental plaque, a complex biofilm system established by cariogenic bacteria such as Streptococcus mutans (S. mutans), is the initiator of dental caries. Studies have found that the cell-free supernatant (CFS) of Lactobacilli could inhibit S. mutans biofilm formation. However, the main antibiofilm substance of the Lactobacilli CFS that acts against S. mutans is unclear. The present study found that the CFS of Lactobacillus plantarum (L. plantarum) ATCC 14917 had the strongest antibiofilm effect among the five tested oral Lactobacilli. Further bioassay-guided isolation was performed to identify the main antibiofilm substance. The antibiofilm effect of the end product, named 1-1-4-3, was observed and the structure of it was elucidated by using Q-TOF MS, 2D NMR and HPLC. The results showed that several components in the CFS had an antibiofilm effect; however, the effect of 1-1-4-3 was the strongest, as it could reduce the generation of exopolysaccharides and make the biofilm looser and thinner. After structure elucidation and validation, 1-1-4-3 was identified as a mixture of lactic acid (LA) and valine. Additionally, LA was shown to be the main antibiofilm substance in 1-1-4-3. In summary, this study found that the antibiofilm effect of the L. plantarum CFS against S. mutans was attributable to the comprehensive effect of multiple components, among which LA played a dominant role.
Morphological and Functional Remodeling of Vascular Endothelium in Cardiovascular Diseases
Authors: Bkaily Ghassan , Jacques Danielle
Published: January 2023

The vascular endothelium plays a vital role during embryogenesis and aging and is a cell monolayer that lines the blood vessels. The immune system recognizes the endothelium as its own. Therefore, an abnormality of the endothelium exposes the tissues to the immune system and provokes inflammation and vascular diseases such as atherosclerosis. Its secretory role allows it to release vasoconstrictors and vasorelaxants as well as cardio-modulatory factors that maintain the proper functioning of the circulatory system. The sealing of the monolayer provided by adhesion molecules plays an important role in cardiovascular physiology and pathology.
Diffusion/Reaction Limited Aggregation Approach for Microstructure Evolution and Condensation Kinetics during Synthesis of Silica-Based Alcogels
Authors: Borzęcka Nina Helena , Nowak Bartosz , Pakuła Rafał , Przewodzki Robert , Gac Jakub Maksymilian
Published: January 2023

A base-catalysed methyltrimethoxysilane (MTMS) colloidal gel formation was implemented as a cellular automaton (CA) system, specifically diffusion and/or reaction-limited aggregation. The initial characteristic model parameters were determined based on experimental synthesis of MTMS-based, ambient-pressure-dried aerogels. The applicability of the numerical approach to the prediction of gels’ condensation kinetics and their structure was evaluated. The developed model reflects the kinetics properly within the investigated chemical composition range (in strongly reaction-limited aggregation conditions) and, to a slightly lesser extent, the structural properties of aggregates. Ultimately, a relatively simple numerical model reflecting silica-based gel formation was obtained and verified experimentally. The CA simulations have proved valid for understanding the relation between the initial chemical composition and kinetics constants of MTMS-based synthesis and their impact on secondary particle aggregation process kinetics.
Glial Cultures Differentiated from iPSCs of Patients with PARK2-Associated Parkinson’s Disease Demonstrate a Pro-Inflammatory Shift and Reduced Response to TNFα Stimulation
Authors: Gerasimova Tatiana , Stepanenko Ekaterina , Novosadova Lyudmila , Arsenyeva Elena , Shimchenko Darya , Tarantul Vyacheslav , Grivennikov Igor , Nenasheva Valentina , Novosadova Ekaterina
Published: January 2023

Parkinson’s disease (PD) is the second most common neurodegenerative diseases characterized by progressive loss of midbrain dopaminergic neurons in the substantia nigra. Mutations in the PARK2 gene are a frequent cause of familial forms of PD. Sustained chronic neuroinflammation in the central nervous system makes a significant contribution to neurodegeneration events. In response to inflammatory factors produced by activated microglia, astrocytes change their transcriptional programs and secretion profiles, thus acting as immunocompetent cells. Here, we investigated iPSC-derived glial cell cultures obtained from healthy donors (HD) and from PD patients with PARK2 mutations in resting state and upon stimulation by TNFα. The non-stimulated glia of PD patients demonstrated higher IL1B and IL6 expression levels and increased IL6 protein synthesis, while BDNF and GDNF expression was down-regulated when compared to that of the glial cells of HDs. In the presence of TNFα, all of the glial cultures displayed a multiplied expression of genes encoding inflammatory cytokines: TNFA, IL1B, and IL6, as well as IL6 protein synthesis, although PD glia responded to TNFα stimulation less strongly than HD glia. Our results demonstrated a pro-inflammatory shift, a suppression of the neuroprotective gene program, and some depletion of reactivity to TNFα in PARK2-deficient glia compared to glial cells of HDs.
Targeting Agents in Biomaterial-Mediated Bone Regeneration
Authors: Gisbert-Garzarán Miguel , Gómez-Cerezo María Natividad , Vallet-Regí María
Published: January 2023

Bone diseases are a global public concern that affect millions of people. Even though current treatments present high efficacy, they also show several side effects. In this sense, the development of biocompatible nanoparticles and macroscopic scaffolds has been shown to improve bone regeneration while diminishing side effects. In this review, we present a new trend in these materials, reporting several examples of materials that specifically recognize several agents of the bone microenvironment. Briefly, we provide a subtle introduction to the bone microenvironment. Then, the different targeting agents are exposed. Afterward, several examples of nanoparticles and scaffolds modified with these agents are shown. Finally, we provide some future perspectives and conclusions. Overall, this topic presents high potential to create promising translational strategies for the treatment of bone-related diseases. We expect this review to provide a comprehensive description of the incipient state-of-the-art of bone-targeting agents in bone regeneration.
Altered Fatty Acid Oxidation in Lymphocyte Populations of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome
Authors: Maya Jessica , Leddy Sabrina M. , Gottschalk C. Gunnar , Peterson Daniel L. , Hanson Maureen R.
Published: January 2023

Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a disabling multisystem illness in which individuals are plagued with fatigue, inflammatory symptoms, cognitive dysfunction, and the hallmark symptom, post-exertional malaise. While the cause of this disease remains unknown, there is evidence of a potential infectious component that, along with patient symptoms and common onsets of the disease, implicates immune system dysfunction. To further our understanding of the state of ME/CFS lymphocytes, we characterized the role of fatty acids in isolated Natural Killer cells, CD4+ T cells, and CD8+ T cells in circulation and after overnight stimulation, through implicit perturbations to fatty acid oxidation. We examined samples obtained from at least 8 and as many as 20 subjects for immune cell fatty acid characterization in a variety of experiments and found that all three isolated cell types increased their utilization of lipids and levels of pertinent proteins involved in this metabolic pathway in ME/CFS samples, particularly during higher energy demands and activation. In T cells, we characterized the cell populations contributing to these metabolic shifts, which included CD4+ memory cells, CD4+ effector cells, CD8+ naïve cells, and CD8+ memory cells. We also discovered that patients with ME/CFS and healthy control samples had significant correlations between measurements of CD4+ T cell fatty acid metabolism and demographic data. These findings provide support for metabolic dysfunction in ME/CFS immune cells. We further hypothesize about the consequences that these altered fuel dependencies may have on T and NK cell effector function, which may shed light on the illness’s mechanism of action.
Acknowledgment to the Reviewers of Informatics in 2022
Authors:   Informatics Editorial Office
Published: January 2023

High-quality academic publishing is built on rigorous peer review [...]
The Influence of Different Recombination Pathways on Hysteresis in Perovskite Solar Cells with Ion Migration
Authors: Li Biao , Chen Kun , Hang Pengjie , Yao Yuxin , Kan Chenxia , Hu Zechen , Wang Ying , Zhang Yiqiang , Yang Deren , Yu Xuegong
Published: January 2023

The impact of hysteresis on the power conversion efficiency (PCE) of perovskite solar cells (PSCs) still faces uncertainties despite the rapid development of perovskite photovoltaics. Although ion migration in perovskites is regarded as the chief culprit for hysteresis, charge carrier recombination pathways in PSCs are proposed to be necessary for the occurrence of hysteresis. Here, the impact of both bulk recombination and interface recombination on hysteresis in PSCs is investigated via drift–diffusion modeling. The simulation results demonstrate a direct correlation between recombination pathways and hysteresis in PSCs with ion migration. The simulation reveals that recombination pathways in PSCs will react to the variation in charge carrier distribution under different voltage scanning directions induced by ion migration in absorber layers, which leads to hysteresis in PSCs. Moreover, the hysteresis in normal (N-I-P) PSCs with different electron transport layers (ETLs) including sintered SnO2, SnO2 nano crystals and TiO2 is experimentally explored. The results demonstrate that multiple recombination pathways coupled with ion migration can lead to obvious hysteresis in fabricated PSCs which is consistent with simulation results. This work provides great insight into hysteresis management upon composition, additive and interface engineering in PSCs.
Furcanthicus gen. nov., a New Genus of Oriental Anthicini (Coleoptera, Anthicidae), with Description of Three New Species
Authors: Zhao Yuchen , Wang Xinpu , Lee Seunghyun , Bai Ming
Published: January 2023

Furcanthicus gen. nov. (Anthicinae: Anthicini) and three new species from the Oriental region are described: Furcanthicus acutibialis sp. nov. (China: Tibet), F. telnovi sp. nov. (China: Yunnan), and F. validus sp. nov. (China: Sichuan). Some critical morphological characters of this genus are discussed. Eight new combinations are established for the following taxa: Furcanthicus punctiger (Krekich-Strassoldo, 1931) comb. nov., F. rubens (Krekich-Strassoldo, 1931) comb. nov., F. maderi (Heberdey, 1938) comb. nov., F. monstrator (Telnov, 2005) comb. nov., F. vicarius (Telnov, 2005) comb. nov., F. lepcha (Telnov, 2018) comb. nov., F. vicinor (Telnov, 2018) comb. nov. (all from Anthicus Paykull, 1798), and Nitorus lii (Uhmann, 1997) comb. nov. (from Pseudoleptaleus Pic, 1900). Two informal species-groups are established: F. maderi and F. rubens species-group. The little-known species F. maderi, F. rubens, and F. punctiger are redescribed, diagnosed, and illustrated. The distribution map and key to species of this new genus are provided.
Fermented or Floral? Developing a Generalized Food Bait Lure to Monitor Cutworm and Armyworm Moths (Lepidoptera: Noctuidae) in Field Crops
Authors: Batallas Ronald E. , Evenden Maya L.
Published: January 2023

Cutworms and armyworms (Lepidoptera: Noctuidae) are a pest complex in North America that cause sporadic damage in field crops on the Canadian Prairies; however, no methods have been developed to reliably monitor population densities. Food-based semiochemicals attract both sexes of adult moths and could be used to monitor multiple species with a single lure in a single trap. Here, we focus on enhancing the attractiveness of acetic acid and 3-methyl-1-butanol (AAMB) lures to redbacked cutworm (Euxoa ochrogaster) (RBC) and other noctuid pests. Experiments conducted in canola and wheat fields tested AAMB lures at different release rates, from different devices and in combination with other semiochemicals. High-release lures captured more females in canola, while low-release lures captured more males in wheat. Thus, crop volatiles may influence response to lures. Semiochemicals embedded in an inert matrix caught more RBC moths than semiochemicals released from Nalgene or polyethylene dispensers did. More RBC females were attracted to AAMB lures with 2-methyl-1-propanol than phenylacetaldehyde. Fermented volatiles appear to be a more reliable attractant than floral volatiles for these species. RBC moth antennae produced significant responses to all doses of phenylacetaldehyde tested in electroantennogram assays, but only to higher doses of acetic acid and 3-methyl-1-butanol. Physiological state of the RBC moths also influenced responsiveness to the tested semiochemical. Feeding status did not influence the antennal response to acetic acid and phenylacetaldehyde in either sex, but it increased the response to 3-methyl-1-butanol in females when fed. AAMB lures should be further developed to monitor RBC moths and other noctuid pests in field crops.
Laboratory Evidence of 2-Isobutyl-3-methoxypyrazine as a Male-Released Aggregative Cue in Labidostomis lusitanica (Germar) (Coleoptera: Chrysomelidae)
Authors: López Sergio , Rodrigo-Gómez Sara , Fernández-Carrillo Enrique , Corbella-Martorell Clàudia , Quero Carmen
Published: January 2023

In spite of its incidence on pistachio trees, the chemical ecology of Labidostomis lusitanica (Germar) (Coleoptera: Chrysomelidae) has been neglected so far. In this work, we provide the first evidence of a biologically active male-specific compound that may be promoting field aggregation. Headspace collections through solid-phase microextraction from feral males and females reported the presence of 2-isobutyl-3-methoxypyrazine exclusively in males. Electroantennographic recordings revealed that males and females responded in a dose-dependent manner to increasing stimuli of 2-isobutyl-3-methoxypyrazine, with females overall displaying a higher response than males. In dual-choice tests, both males and females showed a significant preference for the compound in comparison to a pure air stimulus. In light of these results, the possible role of 2-isobutyl-3-methoxypyrazine as an aggregation cue in L. lusitanica is discussed.
Joint FAO/IAEA Coordinated Research Project on “Mosquito Handling, Transport, Release and Male Trapping Methods” in Support of SIT Application to Control Mosquitoes
Authors: Gómez Maylen , Johnson Brian J. , Bossin Hervé C. , Argilés-Herrero Rafael
Published: January 2023

Mosquito-borne diseases are among the most important public health problems worldwide [...]
Lipoprotein(a), Cardiovascular Events and Sex Differences: A Single Cardiological Unit Experience
Authors: Dal Pino Beatrice , Gorini Francesca , Gaggini Melania , Landi Patrizia , Pingitore Alessandro , Vassalle Cristina
Published: January 2023

Lipoprotein(a)-Lp(a), which retains proatherogenic and prothrombotic properties, may be modified by hormonal and metabolic factors. However, few studies have focused on differences related to sex and cardiometabolic risk factors in the relationship between Lp(a) and cardiovascular disease, especially in terms of prognosis. This study aimed at evaluating the predictive value of Lp(a) (cut-off 30 mg/dL) for hard events (HEs: mortality and non-fatal myocardial infarction) according to sex and cardiometabolic risk factors in 2110 patients (1501 males, mean age: 68 ± 9 years) undergoing coronary angiography for known or suspected coronary artery disease. There were 211 events over a median follow-up period of 33 months. Lp(a) > 30 mg/dL did not confer a worse prognosis on the overall population. However, Kaplan–Meier subgroup analysis evidenced a worse prognosis in type 2 diabetes (T2D) females with elevated Lp(a) (log-rank test: p = 0.03) vs. T2D males and no-T2D patients, but not in other high-risk cardiovascular states (e.g., smoking, hypertension, reduced left ventricular ejection fraction or obesity). After Cox multivariate adjustment, Lp(a) remained an independent determinant for HEs in the T2D female subgroup, conferring an HR of 2.9 (95% CI 1.1–7.7, p < 0.05). Lp(a) is therefore a strong independent predictor of HR in T2D women, but not in T2D men, or in noT2D patients.
Mitochondria: Emerging Consequential in Sickle Cell Disease
Authors: Akhter Mohammad S. , Hamali Hassan A. , Rashid Hina , Dobie Gasim , Madkhali Aymen M. , Mobarki Abdullah A. , Oldenburg Johannes , Biswas Arijit
Published: January 2023

Advanced mitochondrial multi-omics indicate a multi-facet involvement of mitochondria in the physiology of the cell, changing the perception of mitochondria from being just the energy-generating organelles to organelles that highly influence cell structure, function, signaling, and cell fate. This sets mitochondrial dysfunction in the centerstage of numerous acquired and genetic diseases. Sickle cell disease is also being increasingly associated with mitochondrial anomalies and the pathophysiology of sickle cell disease finds mitochondria at crucial intersections in the pathological cascade. Altered mitophagy, increased ROS, and mitochondrial DNA all contribute to the condition and its severity. Such mitochondrial aberrations lead to consequent mitochondrial retention in red blood cells in sickle cell diseases, increased oxidation in the cellular environment, inflammation, worsened vaso-occlusive crisis, etc. There are increasing studies indicating mitochondrial significance in sickle cell disease, consequently providing an opportunity to target it for improving the outcomes of treatment. Identification of the impaired mitochondrial attributes in sickle cell disease and their modulation by therapeutic interventions can impart a better management of the disease. This review aims to describe the mitochondria in the perspective of sicke cell disease so as to provide the reader an overview of the emerging mitochondrial stance in sickle cell disease.
CD8 Encephalitis in HIV: A Review of This Emerging Entity
Authors: Shenoy Aniruddh , Marwaha Pavan Kaur , Worku Dominic Adam
Published: January 2023

Introduction: Encephalitis is a life-threatening neurological condition with multiple causes in the setting of Human Immunodeficiency Virus (HIV). CD8 Encephalitis (CD8E) is a newly recognised condition which can present in an acute manner, with pertinent features including classical radiological findings with an intense brain parenchymal infiltration of CD8+ T cells. This review attempted to clarify the symptomatology, distribution and determinants of this condition, as well as to examine its vast unknowns. Methods: A literature review was undertaken in July 2022, utilising the PubMed and Google Scholar databases. Papers published between 2006–2022 were reviewed. Eighteen papers, totalling 57 patients, were found and analysed. Statistical analysis was undertaken using Chi-squared and Wilcoxon rank-sum tests as appropriate, with p < 0.05 deemed significant. Results: In this review, 57 patients were identified, with a female (61%, 34/56) and Black African (70%, 40/57) preponderance. Females were more likely to present with headache (p = 0.006), and headache was more likely to be present in those who died (p = 0.02). There was no statistically significant association between baseline CD4 count (p = 0.079) and viral load (p = 0.72) with disease outcome. Overall, 77% (41/53) of patients had classical imaging findings, including bilateral gadolinium-enhancing punctate and perivascular white matter lesions. However, many patients (23/57) required a brain biopsy as part of their diagnostic workup. Corticosteroid treatment was commonly prescribed in patients (64%, 35/55) and had a mortality benefit, with an overall survival in this group of 71% (p = 0.0008). In those who died, median survival was 5.5 months. In rare instances, recurrence of the disease was noted, which responded poorly to treatment. Discussion: CD8E represents a new and complex condition with few risk factors identified for its occurrence. The presenting symptoms are broad, but headache appears to be more common in females and more significantly associated with death. Though rare, CD8E is likely under-diagnosed, possibly due to overlapping features with other illnesses and lack of physician experience in its recognition and management. Corticosteroids demonstrate a clear mortality benefit, but more studies are required to determine their optimal dosing and duration, as well as the use of steroid-sparing agents. Further reviews should help to better determine the risk factors for the condition, as well as non-invasive biomarkers, to aid in diagnosis and help to predict poor prognosis and disease recurrence.
Physical Activity Influences Health-Related Quality of Life in Adults with Juvenile Idiopathic Arthritis
Authors: de Oliveira Rodrigo Joel , Londe Ana Carolina , de Souza Débora Pessoa , Marini Roberto , Fernandes Paula Teixeira , Appenzeller Simone
Published: January 2023

This cross-sectional study aimed to evaluate the impact of physical activity and physical fitness on the health-related quality of life (HQoL) of adult patients with Juvenile Idiopathic Arthritis (JIA). Fifty-nine JIA patients and sixty healthy individuals participated in this study. All individuals had the following evaluations performed: body composition (electrical bioimpedance), physical fitness (6 min walk test (6MWT)), physical activity level (International Physical Activity Questionnaire (IPAQ)), and HQoL (Quality of Life Questionnaire in relation to Health—Short Form (SF36)). Thirty-nine (66%) JIA patients were considered sedentary compared with 15 (25%) in the control group (p < 0.01). JIA patients had a lower HQoL compared with the control group in all variables studied (p < 0.05). JIA patients who were very physically active had better HQoL conditions in the categories of functional capacity (p = 0.001), limitations by physical aspects (p = 0.003), and emotional aspects (p = 0.002) compared with sedentary patients. JIA patients had more cardiovascular abnormalities and walked shorter distances compared with healthy controls in the 6MWT. In conclusion, we observed that HQoL was reduced in adults with JIA. A high percentage of JIA patients were sedentary with lower physical fitness, but physically active patients had a better HQoL than sedentary patients. The duration of physical activity, rather than intensity, influenced the mental aspects of HQoL.
Effects of an Exercise for Well-Being and Physical Training Programme on Muscle Strength, Range of Movement, Respiratory Capacity and Quality of Life in Women with Fibromyalgia: A Randomized Controlled Trial
Authors: Rodríguez-Mansilla Juan , Mejías-Gil Abel , Garrido-Ardila Elisa María , Jiménez-Palomares María , Montanero-Fernández Jesús , González-López-Arza María Victoria
Published: January 2023

The objective of this study was to assess the efficacy of an active exercise physiotherapy programme versus an exercise for well-being programme improving muscle strength, range of movement, respiratory capacity and quality of life of women with fibromyalgia. A randomized, assessor-blind, controlled trial was conducted. A total of 141 women diagnosed with fibromyalgia were randomized to a physiotherapy exercise group (n = 47), an exercise for well-being group (n = 47) and a control group (n = 47). The study lasted 4 weeks and the experimental groups received 45 min sessions performed twice a week on alternate days. The primary outcome measures were range of movement and muscle strength. The secondary outcome measures were respiratory capacity and quality of life. The results showed statistically significant improvements in the exercise for well-being and physiotherapy groups vs. the control group at week 5 in relation to joint range of movement (p = 0.004), muscle strength (p = 0.003) and quality of life (p = 0.002). The changes found in all the spirometry parameters seem to be associated to some of the changes in joint range of movement and muscle strength as well as quality of life. Physiotherapy and exercise for well-being improved upper limb and lower limb range of movement and the muscle strength of women with fibromyalgia.
Incidence of Isolated Biliary Atresia during the COVID Lockdown in Europe: Results from a Collaborative Project by RARE-Liver
Authors: Nomden Mark , Alizai Naved , Betalli Pietro , Bruggink Janneke , Cananzi Mara , Christensen Vibeke , D’Antiga Lorenzo , Davenport Mark , Fischler Björn , Hindemith Luise , Hukkinen Maria , Johansen Lars , de Kleine Ruben , Madadi-Sanjani Omid , Ong Evelyn , Pakarinen Mikko , Petersen Claus , Ruiz Mathias , Schunn Matthias , Sturm Ekkehard , Verkade Henkjan , Wildhaber Barbara , Hulscher Jan ,   on behalf of members of the Biliary Atresia and Related Disorders (BARD) Community & the ERN RA-RE-LIVER
Published: January 2023

Background: Biliary atresia (BA) is a rare cholangiopathy where one of the proposed aetiological mechanisms is an infectious viral trigger. Coronavirus disease-19 (COVID) lockdown restrictions were implemented to reduce the transmission of infections. Strictness of lockdown varied across European countries. This study aimed to investigate if there was an association between strictness of lockdown and change in isolated BA (IBA) incidence in Europe. Methods: We approached European centres involved in the European Reference Network RARE-LIVER. We included IBA patients born between 2015 and June 2020. We calculated the number of IBA patients born per centre per month. The Stringency Index (SI) was used as lockdown strictness indicator. The association between percentage change of mean number of IBA patients born per month and the SI was assessed. Results: We included 412 IBA patients from thirteen different centres. The median number of patients per month did not change: 6 (1–15) pre-lockdown and 7 (6–9) during lockdown (p = 0.34). There was an inverse association between SI and percentage change in IBA (B = -0.73, p = 0.03). Median age at Kasai portoenterostomy (days) did not differ between time periods (51 (9–179) vs. 53 (19–126), p = 0.73). Conclusion: In this European study, a stricter COVID-lockdown was seemingly accompanied by a simultaneous larger decrease in the number of IBA patients born per month in the lockdown. Results should be interpreted with caution due to the assumptions and limitations of the analysis.
Validation of Physical Activity Correlates Questionnaire from Social Ecological Model in College Students
Authors: Huang Meirong , Sun Haichun , Chen Han , Zhang Yanping , Adams Kaitlyn , Gao Zan
Published: January 2023

More than 50% of Chinese college students rarely engage in any type of physical activity (PA). The literature shows several issues in college students’ health due to lack of PA. Promoting college students’ PA participation across the country has become a challenging task. This study aims to create a questionnaire into the correlates that affect Chinese college students’ engagement in leisure time PA. We first determined the factor structure of college students’ PA engagements. Then, we tested and verified the reliability and validity of the factor structure of the questionnaire. The Chinese college students’ PA correlates questionnaire was designed according to the Social Ecological Model. Exploratory factor analysis (EFA) extracted all the included items, whereas confirmatory factor analysis (CFA) verified the validity of the model. We recruited 1290 college students to complete the questionnaire. A second-order structural model was constructed. Specifically, the first-order included individual, social, and environmental as dimensions, while the second-order had interest, media, social support, traditional culture, facilities, and environment as factors. The six factors have polymerized 22 items. The model demonstrated a good fit (X2/df = 1.4; CFI = 0.98; GFI = 0.94; RMSEA = 0.02; SRMR = 0.05). In conclusion, the newly established questionnaire of college students’ PA correlates is reasonable, and the results of factors reliabilities and model fit are acceptable.
Colonic Transendoscopic Enteral Tubing Is a New Pathway to Microbial Therapy, Colonic Drainage, and Host–Microbiota Interaction Research
Authors: Wang Weihong , Lu Gaochen , Wu Xia , Wen Quan , Zhang Faming
Published: January 2023

The limitation of traditional delivery methods for fecal microbiota transplantation (FMT) gave birth to colonic transendoscopic enteral tubing (TET) to address the requirement of frequent FMTs. Colonic TET as a novel endoscopic intervention has received increasing attention in practice since 2015 in China. Emerging studies from multiple centers indicate that colonic TET is a promising, safe, and practical delivery method for microbial therapy and administering medication with high patient satisfaction. Intriguingly, colonic TET has been used to rescue endoscopy-related perforations by draining colonic air and fluid through the TET tube. Recent research based on collecting ileocecal samples through a TET tube has contributed to demonstrating community dynamics in the intestine, and it is expected to be a novel delivery of proof-of-concept in host–microbiota interactions and pharmacological research. The present article aims to review the concept and techniques of TET and to explore microbial therapy, colonic drainage, and microbial research based on colonic TET.
Influence of LDL-Cholesterol Lowering on Coronary Plaque Progression of Non-Target Lesions in Patients Undergoing Percutaneous Coronary Intervention: Findings from a Retrospective Study
Authors: Quan Weiwei , Han Hui , Liu Lili , Sun Yi , Zhu Zhengbin , Du Run , Zhu Tianqi , Zhang Ruiyan
Published: January 2023

The progression of NTLs after PCI accounts for a significant portion of future adverse cardiac events. The reduction in LDL-C reduces cardiovascular events. This has, however, not yet been shown in a real-world setting. We aimed to investigate the association between LDL-C changes with progression in NTLs. A total of 847 patients with successful PCI were enrolled. Patients with follow-up LDL-C ≥ 1.4 mmol/L or percent reduction <50% compared to baseline were Non-optimal group (n = 793); patients with follow-up LDL-C < 1.4 mmol/L and percent reduction ≥50% compared to baseline were Optimal group (n = 54). Compared to Non-optimal group, Optimal group presented a lower rate of NTL plaque progression (11.11% vs. 23.96%; p = 0.007) and a lower follow-up TC (2.77 ± 0.59 vs. 3.66 ± 0.97; p < 0.001) and LDL-C (1.09 ± 0.26 vs. 2.03 ± 0.71; p < 0.001). The univariate logistic regression analysis revealed that follow-up LDL-C < 1.4 mmol/L and a percent reduction ≥50% from baseline was a protective factor for NTL plaque progression (OR: 0.397; 95%CI: 0.167–0.941; p = 0.036). The multivariate logistic regression model revealed that follow-up LDL-C < 1.4 mmol/L and percent reduction ≥50% was indeed an independent factor associated with a lower rate of plaque progression of NTLs (OR: 0.398; 95% CI: 0.167–0.945; p = 0.037). Therefore, achieving guideline-recommended LDL-C level was associated with a significantly reduced risk of NTL plaque progression.
How to Solve the Conundrum of Heparin-Induced Thrombocytopenia during Cardiopulmonary Bypass
Authors: Revelly Etienne , Scala Emmanuelle , Rosner Lorenzo , Rancati Valentina , Gunga Ziyad , Kirsch Matthias , Ltaief Zied , Rusca Marco , Bechtold Xavier , Alberio Lorenzo , Marcucci Carlo
Published: January 2023

Heparin-induced thrombocytopenia (HIT) is a major issue in cardiac surgery requiring cardiopulmonary bypass (CPB). HIT represents a severe adverse drug reaction after heparin administration. It consists of immune-mediated thrombocytopenia paradoxically leading to thrombotic events. Detection of antibodies against platelets factor 4/heparin (anti-PF4/H) and aggregation of platelets in the presence of heparin in functional in vitro tests confirm the diagnosis. Patients suffering from HIT and requiring cardiac surgery are at high risk of lethal complications and present specific challenges. Four distinct phases are described in the usual HIT timeline, and the anticoagulation strategy chosen for CPB depends on the phase in which the patient is categorized. In this sense, we developed an institutional protocol covering each phase. It consisted of the use of a non-heparin anticoagulant such as bivalirudin, or the association of unfractionated heparin (UFH) with a potent antiplatelet drug such as tirofiban or cangrelor. Temporary reduction of anti-PF4 with intravenous immunoglobulins (IvIg) has recently been described as a complementary strategy. In this article, we briefly described the pathophysiology of HIT and focused on the various strategies that can be applied to safely manage CPB in these patients.
Effects of Physiotherapy on Pain and Mouth Opening in Temporomandibular Disorders: An Umbrella and Mapping Systematic Review with Meta-Meta-Analysis
Authors: Arribas-Pascual Manuel , Hernández-Hernández Sofia , Jiménez-Arranz Christian , Grande-Alonso Mónica , Angulo-Díaz-Parreño Santiago , La Touche Roy , Paris-Alemany Alba
Published: January 2023

The aim of this meta-meta-analysis was to assess the current evidence regarding the effect of physical therapy (PT) interventions on pain and functional variables in temporomandibular disorders (TMD). We conducted an umbrella systematic review (SR) and four meta-meta-analyses (MMA) and created an evidence map to determine the effectiveness of PT on pain intensity and maximum mouth opening in patients with TMD. The quality of the included SR was assessed with the AMSTAR 2, and the risk of bias with ROBIS. Of the 31 SR included in the umbrella SR, only 10 were included in the MMA. The MMA showed moderate effects for manual therapy and therapeutic exercise, and large effects for low-level laser therapy on improving pain intensity and maximum mouth opening in patients with TMD, with a limited to moderate quality of evidence. The overlapping analyses showed only a slight overlap for all the MMA according to the corrected covered area (range from 0.07 to 0.2), 23.1% to 41.6%. This umbrella SR showed that manual therapy and exercise interventions, as well as low-level laser therapy interventions, are effective in the reduction in pain intensity and improvement of maximum mouth opening in TMD. This article presents a synthesis of the available evidence related to the various physical therapy interventions used in patients presenting with temporomandibular disorders. These results could help clinicians to select the optimal intervention for their patients and to reject those that are less useful.
Can Color Doppler Ultrasound Be Effectively Used as the Follow-Up Modality in Patients Undergoing Splenic Artery Aneurysm Embolization? A Correlational Study between Doppler Ultrasound, Magnetic Resonance Angiography and Digital Subtraction Angiography
Authors: Lamparski Krzysztof , Procyk Grzegorz , Bartnik Krzysztof , Korzeniowski Krzysztof , Maciąg Rafał , Matsibora Vadym , Sajdek Michał , Dryjańska Alicja , Wnuk Emilia , Rosiak Grzegorz , Maj Edyta , Januszewicz Magdalena , Gąsecka Aleksandra , Ostrowski Tomasz , Kaszczewski Piotr , Gałązka Zbigniew , Wojtaszek Mikołaj
Published: January 2023

Splenic artery aneurysm (SAAs) rupture is associated with a high mortality rate. Regular surveillance with imaging before and after intervention is crucial to guide best evidence treatment. The following study aimed to determine the efficacy of color Doppler ultrasound imaging (DUS) compared to digital subtraction angiography (DSA) and magnetic resonance angiography (MRA) as a follow-up modality after selective coil embolization of true SAAs. We analyzed data from 20 patients, 15 females (48.1 ± 16.1 years) undergoing selective SAA coil embolization using detachable fibered embolization coils. Imaging using DUS, MRA, and DSA was performed 3 months after the initial embolization or the consequent re-embolization procedure. Primary clinical success, defined as Class I aneurysm occlusion, on 3-month follow-up was seen in 16 (80.0%) patients. DUS had a sensitivity of 94.4% and a specificity of 42.9% when compared to DSA and 92.3% and 30%, respectively, when compared to MRA in identifying Class I aneurysm occlusion. The positive predictive value (PPV) of DUS in identifying the need for re-embolization was 75.0%, while the NPV of DUS in these terms was 90.5%. DUS showed a high sensitivity in detecting aneurysm occlusion and clinical success, simultaneously exhibiting poor specificity. Still, with caution, this follow-up modality could be used for monitoring select low-risk patients after selective embolization of SAAs. DUS could provide a higher cost-to-benefit ratio, enabling more systematic post-procedural follow-up, as it is far more commonly used compared to MRA and non-invasive compared to DSA.
Presbyopia: What We Do Know and What We Do Not Know in 2022
Authors: Grzybowski Andrzej , Gawęcki Maciej
Published: January 2023

The subject of presbyopia has accompanied clinical ophthalmic practices around the world for centuries [...]
Association between Leukemia Inhibitory Factor Gene Polymorphism and Clinical Outcomes among Young Women with Poor Ovarian Response to Assisted Reproductive Technology
Authors: Liu Yung-Liang , Lee Chun-I , Liu Chung-Hsien , Cheng En-Hui , Yang Shun-Fa , Tsai Hsueh-Yu , Lee Maw-Sheng , Lee Tsung-Hsien
Published: January 2023

Background: Does the presence of single-nucleotide polymorphisms (SNPs) in the leukemia inhibitory factor (LIF) gene affect ovarian response in infertile young women? Methods: This was a case–control study recruiting 1744 infertile women between January 2014 to December 2015. The 1084 eligible patients were stratified into four groups using the POSEIDON criteria. The gonadotropin-releasing hormone receptor (GnRHR), follicle-stimulating hormone receptor (FSHR), anti-Müllerian hormone (AMH), and LIF SNP genotypes were compared among the groups. The distributions of LIF and FSHR among younger and older patients were compared. Clinical outcomes were also compared. Results: The four groups of poor responders had different distributions of SNP in LIF. The prevalence of LIF genotypes among young poor ovarian responders differed from those of normal responders. Genetic model analyses in infertile young women revealed that the TG or GG genotype in the LIF resulted in fewer oocytes retrieved and fewer mature oocytes relative to the TT genotypes. In older women, the FSHR SNP genotype contributed to fewer numbers of mature oocytes. Conclusions: LIF and FSHR SNP genotypes were associated with a statistically significant reduction in ovarian response to controlled ovarian hyperstimulation in younger and older women with an adequate ovarian reserve, respectively.