bluuebunny/crossref_metadata_2025 huggingface dataset:
Mechanistic Insights into Selective Hydrogenation of C=C Bonds Catalyzed by CCC Cobalt Pincer Complexes: A DFT Study
Authors: Zuo Zheng , Yang Xinzheng
Published: January 2021

The mechanistic insights into hydrogenations of hex-5-en-2-one, isoprene, and 4-vinylcyclohex-1-ene catalyzed by pincer (MesCCC)Co (Mes = bis(mesityl-benzimidazol-2-ylidene)phenyl) complexes are computationally investigated by using the density functional theory. Different from a previously proposed mechanism with a cobalt dihydrogen complex (MesCCC)Co-H2 as the catalyst, we found that its less stable dihydride isomer, (MesCCC)Co(H)2, is the real catalyst in those catalytic cycles. The generations of final products with H2 cleavages for the formations of C−H bonds are the turnover-limiting steps in all three hydrogenation reactions. We found that the hydrogenation selectivity of different C=C bonds in the same compound is dominated by the steric effects, while the hydrogenation selectivity of C=C and C=O bonds in the same compound could be primarily influenced by the electronic effects. In addition, the observed inhabition of the hydrogenation reactions by excessive addition of PPh3 could be explained by a 15.8 kcal/mol free energy barrier for the dissociation of PPh3 from the precatalyst.
One Step Histological Detection and Staining of the PTEN Tumor Suppressor Protein by a Single Strand DNA
Authors: Longinotti Gloria , Ybarra Gabriel , Vighi Susana , Perandones Claudia , Montserrat Javier , Yakisich Juan Sebastian , Grasselli Mariano , Radrizzani Martin
Published: January 2021

Antibodies are the most used technological tool in histochemistry. However, even with monoclonal antibodies, their standardization is difficult due to variation of biological systems as well as to variability due to the affinity and amplification of the signal arising from secondary peroxidase detection systems. In this article we combined two synthetic molecules to facilitate the standardization of a detection protocol of protein markers in histological sections. The first molecule was an aptamer, a 50-base single-stranded DNA fragment, which recognizes a PTEN tumor suppressor. The second molecule used was also another single stranded 18-base aptamer DNA fragment, which forms a quadruplex structure guanine box. This G-quadruplex recognizes and attaches a molecule of hemin, increasing the catalytic capacity for the hydrogen peroxide. Our results show how the correct structural design of DNA combining an aptamer together with the peroxidase-like DNAzyme allows to detect proteins in histological sections. This tool offers the standardization of the detection of prognostic markers in cancer, in quality and quantity, due to its synthetic nature and its 1:1 antigen:enzyme ratio. This is the first time that reproducible results have been presented in histological sections staining a cancer marker using a single-stranded DNA molecule with dual function.
The Role of TEG Analysis in Patients with COVID-19-Associated Coagulopathy: A Systematic Review
Authors: Hartmann Jan , Ergang Alexis , Mason Dan , Dias Joao D.
Published: January 2021

Coronavirus disease 2019 (COVID-19)-associated coagulopathy (CAC), characterized by hypercoagulability and an increased risk of thrombotic complications, is an important consideration in the management of patients with COVID-19. As COVID-19 is a new disease, no standard of care for the diagnosis or management of its associated coagulopathy is yet established. Whole blood viscoelastic tests, such as thromboelastography (TEG® hemostasis analyzer), analyze whole blood to provide a complete overview of the coagulation status. We conducted a systematic review of thromboelastography for management of patients with COVID-19, using MEDLINE (PubMed) and Cochrane databases. TEG® parameter measurements and clinical outcomes data were extracted for analysis. Our review found 15 publications, with overall results showing thromboelastography can identify and assess a hypercoagulable state in patients with COVID-19. Furthermore, utilization of thromboelastography in this patient population was shown to predict thrombotic complications. The benefits of thromboelastography presented here, in addition to advantages compared with laboratory coagulation tests, position thromboelastography as an important opportunity for optimizing diagnosis of CAC and improving patient management in COVID-19. Given that the benefits of thromboelastography have already been demonstrated in several other clinical applications, we anticipate that clinical data from future studies in patients with COVID-19 will further elucidate the optimal use of thromboelastography in this patient population.
Prognostic Value of 18F–FDG–PET Parameters in Patients with Small Cell Lung Cancer: A Meta-Analysis and Review of Current Literature
Authors: Christensen Tine Nøhr , Andersen Per Kragh , Langer Seppo W. , Fischer Barbara Malene Bjerregaard
Published: January 2021

Many studies have suggested a prognostic value of one or several positron emission tomography (PET) parameters in patients with small cell lung cancer (SCLC). However, studies are often small, and there is a considerable interstudy disagreement about which PET parameters have a prognostic value. The objective of this study was to perform a review and meta-analysis to identify the most promising PET parameter for prognostication. PubMed®, Cochrane, and Embase® were searched for papers addressing the prognostic value of any PET parameter at any treatment phase with any endpoint in patients with SCLC. Pooled hazard ratios (HRs) were calculated by a random effects model for the prognostic value of the baseline maximum standardized uptake value (SUVmax) and metabolic tumor volume (MTV). The qualitative analysis included 38 studies, of these, 19 studies were included in the meta-analyses. The pooled results showed that high baseline MTV was prognostic for overall survival (OS) (HR: 2.83 (95% confidence interval [CI]: 2.00–4.01) and progression-free survival (PFS) (HR: 3.11 (95% CI: 1.99–4.90)). The prognostic value of SUVmax was less pronounced (OS: HR: 1.50 (95% CI: 1.17–1.91); PFS: HR: 1.24 (95% CI: 0.94–1.63)). Baseline MTV is a strong prognosticator for OS and PFS in patients with SCLC. MTV has a prognostic value superior to those of other PET parameters, but whether MTV is superior to other prognosticators of tumor burden needs further investigation.
Employing Industrial Quality Management Systems for Quality Assurance in Outcome-Based Engineering Education: A Review
Authors: Naveed Bin Rais Rao , Rashid Muhammad , Zakria Muaaz , Hussain Sajjad , Qadir Junaid , Imran Muhammad A.
Published: January 2021

With the world becoming flat with fluid boundaries, engineers have to be global in their outlook and their pedigree. Due to the need for international acceptance of engineering qualification, the incorporation of Outcome-Based Education (OBE) has become common and global accreditation treaties such as the Washington Accord have been ratified. Further, it becomes important, especially for an engineering university with a global outlook preparing its students for global markets, to ensure that its graduates attain the planned outcomes. Additionally, the higher education institutions need to make sure that all the stakeholders, including students, parents, employers, and community at large, are getting a quality educational service, where quality is categorized as (1) product-based ensuring that the graduate attained the planned outcomes and skills, and (2) process-based keeping an eye on whether the process is simple, integrated, and efficient. The development of quality movements, such as Total Quality Movement (TQM), Six Sigma, etc., along with quality standards such as ISO 9001 has been instrumental in improving the quality and efficiency in the fields of management and services. Critical to the successful deployment of a quality culture is the institutionalization of an integrated Quality Management System (QMS) in which formally documented processes work according to the Vision and Mission of an institute. At the same time, commitment to Continuous Quality Improvement (CQI) to close the loop through effective feedback, would ensure that the planned outcomes are attained to the satisfaction of all the stakeholders, and that the process overall is improving consistently and continuously. The successful adoption of quality culture requires buy-in from all the stakeholders (and in particular, the senior leadership) and a rigorous training program. In this paper, we provide a review of how a QMS may work for the provision of quality higher education in a 21st-century university.
A Novel Method of EW System Effectiveness Evaluation Based on Conflict Status Association Analysis
Authors: Lu Jianhua , Tang Yanli , Wan Tao , Tang Bin
Published: January 2021

Electronic Warfare (EW) effectiveness evaluation plays an important role in promoting the development of EW technology, promoting the construction of EW equipment systems, and improving the operational capability of the system. Due to the incomplete information caused by the non-cooperative nature of EW, the data of the evaluation index, which are used for effectiveness evaluation, are in Grey scale. In order to approach these problems, we develop a novel method for EW effectiveness evaluation based on Conflict Status Grey Correlation analysis. Specifically, based on conflict theory and correlation theory, an analysis model for the approximation degree of the observation status and expected status of EW is established. Then we introduce the status Grey relational analysis method. The effectiveness value is acquired through the function correlation of the approximation values of both sides, and then we propose an evaluation discrimination method based on status robustness and correlation discrimination. At the same time, we evaluate the effect of errors in the observed values on the evaluation results. The simulation example results show that the proposed model is rational and feasible. This method can effectively overcome the problem of single-angle evaluation of the system and solves the issue of high dependence of traditional EW effectiveness evaluation theory on target data.
Blockchain-Based Reputation Systems: Implementation Challenges and Mitigation
Authors: Battah Ammar , Iraqi Youssef , Damiani Ernesto
Published: January 2021

Reputation expresses the beliefs or opinions about someone or something that are held by an individual or by a community. Reputation Management Systems (RMSs) handle representation, computation, and storage of reputation in some quantitative form, suitable for grounding trust relations among parties. Quantifying reputation is important in situations, like online service provision, which involve interaction between parties who do not know (and potentially distrust) each other. The basic idea is to let parties rate each other. When a party is considered for interaction, its ratings can be aggregated in order to derive a score for deciding whether to trust it or not. While much valuable research work has been done on reputation-based trust schemes, the problem of establishing collective trust in the reputation management system itself has never been fully solved. Recently, several researchers have put forward the idea of using Distributed Ledger Technology (DLT) as the foundation for implementing trustworthy RMSs. The purpose of this paper is to identify some critical problems that arise when DLTs are used in order to manage evidence about previous interaction and compute reputations. The paper proposes some practical solutions and describes methods to deploy them on top of standard DLT of the Ethereum family.
Accessible Visual Artworks for Blind and Visually Impaired People: Comparing a Multimodal Approach with Tactile Graphics
Authors: Cavazos Quero Luis , Iranzo Bartolomé Jorge , Cho Jundong
Published: January 2021

Despite the use of tactile graphics and audio guides, blind and visually impaired people still face challenges to experience and understand visual artworks independently at art exhibitions. Art museums and other art places are increasingly exploring the use of interactive guides to make their collections more accessible. In this work, we describe our approach to an interactive multimodal guide prototype that uses audio and tactile modalities to improve the autonomous access to information and experience of visual artworks. The prototype is composed of a touch-sensitive 2.5D artwork relief model that can be freely explored by touch. Users can access localized verbal descriptions and audio by performing touch gestures on the surface while listening to themed background music along. We present the design requirements derived from a formative study realized with the help of eight blind and visually impaired participants, art museum and gallery staff, and artists. We extended the formative study by organizing two accessible art exhibitions. There, eighteen participants evaluated and compared multimodal and tactile graphic accessible exhibits. Results from a usability survey indicate that our multimodal approach is simple, easy to use, and improves confidence and independence when exploring visual artworks.
Analyzing Femorotibial Cartilage Thickness Using Anatomically Standardized Maps: Reproducibility and Reference Data
Authors: Favre Julien , Babel Hugo , Cavinato Alessandro , Blazek Katerina , Jolles Brigitte M. , Andriacchi Thomas P.
Published: January 2021

Alterations in cartilage thickness (CTh) are a hallmark of knee osteoarthritis, which remain difficult to characterize at high resolution, even with modern magnetic resonance imaging (MRI), due to a paucity of standardization tools. This study aimed to assess a computational anatomy method producing standardized two-dimensional femorotibial CTh maps. The method was assessed with twenty knees, processed following three common experimental scenarios. Cartilage thickness maps were obtained for the femorotibial cartilages by reconstructing bone and cartilage mesh models in tree-dimension, calculating three-dimensional CTh maps, and anatomically standardizing the maps. The intra-operator accuracy (median (interquartile range, IQR) of −0.006 (0.045) mm), precision (0.152 (0.070) mm), entropy (7.02 (0.71) and agreement (0.975 (0.020))) results suggested that the method is adequate to capture the spatial variations in CTh and compare knees at varying osteoarthritis stages. The lower inter-operator precision (0.496 (0.132) mm) and agreement (0.808 (0.108)) indicate a possible loss of sensitivity to detect differences in a setting with multiple operators. The results confirmed the promising potential of anatomically standardized maps, with the lower inter-operator reproducibility stressing the need to coordinate operators. This study also provided essential reference data and indications for future research using CTh maps.
Tumor Necrosis Factor Receptor-Associated Periodic Syndrome (TRAPS) with a New Pathogenic Variant in TNFRSF1A Gene in a Family of the Adult Male with Renal AA Amyloidosis—Diagnostic and Therapeutic Challenge for Clinicians
Authors: Zegarska Jolanta , Wiesik-Szewczyk Ewa , Hryniewiecka Ewa , Wolska-Kusnierz Beata , Soldacki Dariusz , Kacprzak Magdalena , Sobczynska-Tomaszewska Agnieszka , Czerska Kamila , Siedlecki Pawel , Jahnz-Rozyk Karina , Bernatowska Ewa , Zagozdzon Radoslaw , Paczek Leszek
Published: January 2021

Tumor necrosis factor receptor-associated periodic syndrome (TRAPS) belongs to systemic autoinflammatory diseases (AIDs). Many of these syndromes are genetically conditioned and can be inherited. Diagnosis relies on clinical symptoms and should be confirmed by genetic testing. One of the most serious complications is AA amyloidosis. We present the diagnostic route of a 33-year-old male with AA amyloidosis and his children, leading to diagnosis of monogenic autoinflammatory syndrome, confirmed by genetic analysis. A novel variant of the in-frame insertion type in one allele of TNFRSF1A gene was found by whole exome sequencing and confirmed by Sanger sequencing, which allowed a diagnosis of TRAPS. Three-dimensional modeling was used to assess the structural changes introduced into TNFR1 molecule by the insertion. The analysis of the 3D model revealed that accommodation of the 4AA insert induces misalignment of three cysteine bridges (especially the C70-C96 bridge) in the extracellular domain, leading to putatively misfolded and improperly functioning TNFR1. Three of the patient’s daughters inherited the same variant of the TNFRSF1A gene and presented TRAPS symptoms. TRAPS is a very rare disease, but in the presence of suggestive symptoms the genetic diagnostic workout should be undertaken. Early diagnosis followed by appropriate clinical management can prevent irreversible complications.
Current Knowledge and Future Challenges in Takotsubo Syndrome: Part 2—Treatment and Prognosis
Authors: Santoro Francesco , Mallardi Adriana , Leopizzi Alessandra , Vitale Enrica , Rawish Elias , Stiermaier Thomas , Eitel Ingo , Brunetti Natale D.
Published: January 2021

Takotsubo syndrome (TTS) represents a form of acute heart failure featured by reversible left ventricular systolic dysfunction. The management during the acute phase is mainly performed with supportive pharmacological (diuretics, ACE-inhibitors/angiotensin-receptor blockers (ARBs), anticoagulants, antiarrhythmics, non-catecholamine inotropics (levosimendan), and non-pharmacological (mechanical circulatory and respiratory support) therapy, due to the wide clinical presentation and course of the disease. However, there is a gap in evidence and there are no randomized and adequately powered studies on clinical effectiveness of therapeutic approaches. Some evidence supports the use ACE-inhibitors/ARBs at long-term. A tailored approach based on cardiovascular and non-cardiovascular risk factors is strongly suggested for long-term management. The urgent need for evidence-based treatment approaches is also reflected by the prognosis following TTS. The acute phase of the disease can be accompanied by various cardiovascular complications. In addition, long term outcome of TTS patients is also related to non-cardiovascular comorbidities. Physical triggers such as hypoxia and acute neurological disorders in TTS are associated with a poor outcome.
Improving Exposure Assessment Using Non-Targeted and Suspect Screening: The ISO/IEC 17025: 2017 Quality Standard as a Guideline
Authors: Monteiro Bastos da Silva Juliana , Chaker Jade , Martail Audrey , Costa Moreira Josino , David Arthur , Le Bot Barbara
Published: January 2021

The recent advances of novel methodologies such as non-targeted and suspect screening based on high-resolution mass spectrometry (HRMS) have paved the way to a new paradigm for exposure assessment. These methodologies allow to profile simultaneously thousands of small unknown molecules present in environmental and biological samples, and therefore hold great promises in order to identify more efficiently hazardous contaminants potentially associated with increased risks of developing adverse health outcomes. In order to further explore the potential of these methodologies and push the transition from research applications towards regulatory purposes, robust harmonized quality standards have to be implemented. Here, we discuss the feasibility of using ISO/IEC 17025: 2017 as a guideline to implement non-targeted and suspect screening methodologies in laboratories, whether it is for accreditation purposes or not. More specifically, we identified and then discussed how specificities of non-targeted HRMS methodology can be accounted for in order to comply with the specific items of ISO/IEC 17025: 2017. We also discussed other specificities of HRMS methodologies (e.g., need for digital storage capacity) that are so far not included in the ISO/IEC 17025 requirements but should be considered. This works aims to fuel and expand the discussion in order to subsidize new opportunities of harmonization for non-targeted and suspect screening.
Monitoring the Setting Process of Cementitious Materials Using Guided Waves in Thin Rods
Authors: Wang Dongquan , Yu Guangyun , Liu Shukui , Sheng Ping
Published: January 2021

Characterizing early-age properties is very important for the quality control and durability of cementitious materials. In this paper, an approach using embedded guided waves was adopted to monitor the changes in the mechanical proprieties of mortar and concrete during setting, and embedded thin rods with low-cost piezoelectric sensors mounted on top were used for guide wave monitoring. Through continuous attenuation monitoring of the guided waves, the evolution of mortar and concrete properties was characterized. Four different kinds of metallic rods were tested at the same time to find out the optimal setup. Meanwhile, shear wave velocities of the mortar and concrete samples were monitored and correlated to the attenuation, and setting time tests were also performed on these samples. Experimental results demonstrate that the proposed approach could monitor the evolution of the setting of cementitious materials quantitatively, and time of the initial setting could be determined by this technique as well. In addition, it is found that the attenuations of fundamental longitudinal guided wave mode are almost the same in concrete samples and mortar samples sieved from concrete, indicating that this technique is able to eliminate the effects of coarse aggregates, which makes it of great potential for in-situ monitoring of early age concrete.
Morphology Controlled Synthesis of γ-Al2O3 Nano-Crystallites in Al@Al2O3 Core–Shell Micro-Architectures by Interfacial Hydrothermal Reactions of Al Metal Substrates
Authors: Han Dohyeon , Lee Doohwan
Published: January 2021

Fine control of morphology and exposed crystal facets of porous γ-Al2O3 is of significant importance in many application areas such as functional nanomaterials and heterogeneous catalysts. Herein, a morphology controlled in situ synthesis of Al@Al2O3 core–shell architecture consisting of an Al metal core and a porous γ-Al2O3 shell is explored based on interfacial hydrothermal reactions of an Al metal substrate in aqueous solutions of inorganic anions. It was found that the morphology and structure of boehmite (γ-AlOOH) nano-crystallites grown at the Al-metal/solution interface exhibit significant dependence on temperature, type of inorganic anions (Cl−, NO3−, and SO42−), and acid–base environment of the synthesis solution. Different extents of the electrostatic interactions between the protonated hydroxyl groups on (010) and (001) facets of γ-AlOOH and the inorganic anions (Cl−, NO3−, SO42−) appear to result in the preferential growth of γ-AlOOH toward specific crystallographic directions due to the selective capping of the facets by adsorption of the anions. It is hypothesized that the unique Al@Al2O3 core–shell architecture with controlled morphology and exposed crystal-facets of the γ-Al2O3 shell can provide significant intrinsic catalytic properties with enhanced heat and mass transport to heterogeneous catalysts for applications in many thermochemical reaction processes. The direct fabrication of γ-Al2O3 nano-crystallites from Al metal substrate with in-situ modulation of their morphologies and structures into 1D, 2D, and 3D nano-architectures explored in this work is unique and can offer significant opportunities over the conventional methods.
Insight into the Biological Activity of Hennosides—Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead
Authors: Maslovarić Irina , Ilić Vesna , Drvenica Ivana , Stančić Ana , Mojsilović Slavko , Kukolj Tamara , Bugarski Diana , Saso Luciano , Nicoletti Marcello
Published: January 2021

Henna is the current name of the dye prepared from the dry leaf powder of Lawsonia inermis (Lythraceae). Several studies have focused on the chemistry and pharmacology of the henna dyeing active compound, lawsone, obtained from the main constituents of leaves, hennosides, during the processing of plant material. However, knowledge regarding the biological activity of hennosides is largely lacking. In this paper, the redox activity of three hennoside isomers is reported. The pro-oxidative activity was confirmed by their ability to induce mild lysis of erythrocytes and to increase the level of methemoglobin at the concentration ≥ 500 μg/mL. The antioxidant activity of hennosides (concentration ≥100 μg/mL) was determined by FRAP and ABTS assays. At concentration of 500 μg/mL, antioxidant activity of hennoside isomers was equivalent to 0.46 ± 0.08, 0.62 ± 0.28 and 0.35 ± 0.03 mM FeSO4 × 7H2O, and 0.15 ± 0.01, 0.30 ± 0.01 and 0.09 ± 0.01 mM Trolox. Hennosides at 100 μg/mL concentration did not influence viability of human breast cancer cell lines MDA231 and MCF-7 and primary human peripheral blood and periodontal ligament-mesenchymal stem cells, but produced a modest increase in concentration of antioxidants in the cell culture supernatants. The evidenced antioxidant and pro-oxidant activities indicate their potential to act as redox balance regulator, which opens up the possibility of using hennosides in commercial phytomedicines.
BEHAVIORAL ACTIVITY OF COWS IN THE CONDITIONS OF ROBOTIC MILKING AND ITS RELATIONSHIP TO DAIRY PRODUCTIVITY
Authors: ЗАГИДУЛЛИН Л.Р. , ХИСАМОВ Р.Р. , ШАЙДУЛЛИН Р.Р. , КАЮМОВ Р.Р. , НИГМАТЗЯНОВ С.М.
Published: January 2021

Приведены результаты исследования этологии коров Татарстанского типа холмогорской породы в условиях роботизированного доения. Для анализа результатов рассчитаны индексы пищевой, двигательной и общей активности. Коровы по молочной продуктивности разделены на 3 группы: низкопродуктивные, среднепродуктивные и высокопродуктивные. Индекс пищевой активности в среднем у животных составил 0,542. Минимальная пищевая активность наблюдалась между 7 и 8 ч (0,344), максимальная — между 20 и 21 ч (0,667). Индекс двигательной активности варьировал от 0,222 между 6 и 7 ч до 0,717 между 15 и 16 ч. Индекс общей активности составил 0,751. Животные проявляют определенный вид активности в течение 75% суточного времени. Периоды наибольшей активности наблюдаются в 8—9 ч (0,928), 15—16 (0,878) и 20—21 (0,883) ч. В ночное время коровы менее активны, чем в дневное. Животные доились в среднем 3,8 раза в сутки, при этом низкопродуктивные — 2,8 раза, среднепродуктивные — 3,6, высокопродуктивные — 4,7 раза. Индекс пищевой активности у животных разных групп составляет 0,542—0,543. Однако животные высокопродуктивной группы принимали корм на 54 мин дольше, чем низкопродуктивные. Высокопродуктивные животные больше времени проводили стоя: индексы двигательной активности в группах составили 0,415, 0,528 и 0,556. Самыми активными были животные средней молочной продуктивности, индекс общей активности которых составил 0,762.

The results of a study of the ethology of cows of Kholmogory breed of Tatarstan type in the conditions of robotic milking are presented. To analyze the results, indexes of food, motor and total activity were calculated. Cows on milk productivity are divided into 3 groups: low-productive, medium-productive and high-productive. The index of food activity of animals was 0.542. The minimum of food activity was observed between 7 and 8 hours (0.344), the maximum — between 20 and 21 hours (0.667). The motor activity index ranged from 0.222 between 6 and 7 hours to 0.717 between 15 and 16 hours. The total activity index was 0.751. Animals show a certain type of activity during 75 % of the daily time. The periods of greatest activity are observed in 8—9 (0.928), 15—16 (0.878), and 20—21 (0.883) hours. At night, cows are less active than in the daytime. Animals were milked on average 3.8 times a day, while low-productivity — 2.8 times, medium-productive — 3.6 times, high-productive — 4.7 times. The index of food activity in animals of different groups is 0.542.—.0.543. However, animals of the high-productive group ate for 54 minutes more than the low-productivity ones. Highly productive animals spend more time standing: motor activity indexes in the groups were 0.415, 0.528 and 0.556. The most active are animals of medium milk productivity, the index of total activity of which was 0.762.
Cell-Based Biohybrid Sensor Device for Chemical Source Direction Estimation
Authors: Oda H. , Kihara K. , Morimoto Y. , Takeuchi S.
Published: January 2021

This paper describes a method to estimate the direction from which the signal molecule reaches the sensor by using living cells. In this context, biohybrid sensors that utilize a sophisticated sensing system of cells can potentially offer high levels of chemical-detection sensitivity and selectivity. However, biohybrid-sensor-based chemical-source-direction estimation has not received research attention because the cellular response to chemicals has not been examined in the context of directional information. In our approach, we fabricated a device that can limit the interface between the cell-laden hydrogel and the chemical solution of interest to enhance the time difference over which the chemical solution reaches the cells. Chemical detection by cells that express specific receptors is reflected as the fluorescence of the calcium indicator within the cells. Our device has eight chambers that each house 3D cell-laden collagen hydrogels facing circularly outward. The device also works as a cover to prevent chemicals from permeating the hydrogel from above. In our study, by observing the time course of the fluorescence emission of each chamber, we were able to successfully estimate the chemical-source direction within an error range of 7–13°. Our results suggest that a combination of microstructure devices embedded with living cells can be used to exploit cell functionalities to yield chemical-source directional information.
Makna Takut akan Tuhan Menurut Amsal 8:13 (Studi Evaluasi di Jemaat GPdI “Filadelfia” Oepaleo Kupang)
Authors: Nelly Nelly , Nome Rodi Imenuel
Published: January 2021

Abstract:
Fear of God are hates evil; hates pride, hates conceit, hates evil conduct, and hates guile-filled mouths. In the fear of God is also a sign of the quality and morality of a person who can be accountable for his life before God and shows a view of life that contains human proper attitudes that are always shown in everyday life because of his good and close relationship with God. The purpose of this study was to describe the meaning of fearing God according to Proverbs 8:13 in the GPdI "Filadelfia" congregation in Oepaleo Kupang. This article is a qualitative research with an evaluation approach, the data is processed and analyzed using descriptive statistics. From the results of the percentage of the five indicators that the researcher included in this study, the average percentage value was in the high criteria, namely 61.81%. In other words, the congregation at GPdI "Filadelfia" Oepaleo Kupang already understands the meaning of fearing God, but it needs to be improved.
Aplicación de la teoría de actos propios para la extensión de la cláusula arbitral a partes no signatarias en Bolivia
Authors: Rojas Sangüesa María Fernanda
Published: January 2021

Debido a la complejidad de las relaciones jurídicas, se ha podido evidenciar que, en ocasiones, una parte que no ha suscrito la cláusula arbitral, a través de sus propios actos, ha otorgado su consentimiento. Asimismo, es posible que a lo largo de la relación contractual se incorporen nuevos actores. Es por esto que, la doctrina, ha desarrollado teorías justificativas que reconocen que la cláusula arbitral puede ser extendida de manera excepcional a partes no signatarias. Una de estas es la teoría de los actos propios que comprende el consentimiento tácito y la buena fe. Perú es uno de los países que ha adoptado esta teoría en su Ley de Arbitraje. Al respecto, si bien en Bolivia se reconoce el consentimiento tácito, no se han determinado sus alcances en materia arbitral, y tampoco se reconoce expresamente dicha teoría en la Ley de Conciliación y Arbitraje. Razón por la cual, se realizó un análisis, y se establecieron las condiciones de compatibilidad jurídica entre ambos países para determinar cuáles son los alcances y criterios que se deben tomar en cuenta para adoptar o no esta teoría en nuestro país, a fin de evitar su incorrecta aplicación que ocasionaría una violación a la seguridad jurídica de las partes.
La sede del arbitraje
Authors: Villarroel Diego
Published: January 2021

En este ensayo se analiza el concepto de la sede del arbitraje y su importancia tanto teórica como práctica en el contexto del derecho arbitral. La relevancia de la sede del arbitraje se pone de manifiesto cuando identificamos sus alcances: define la nacionalidad del laudo arbitral, la lex arbitri y los tribunales judiciales que intervendrán en el arbitraje (ya sea para tareas de control o auxilio judicial).
Improving Teacher Pedagogy In the Creation of IT-Based Learning Media
Authors: Simanjuntak Christina , Nunuk Hariyati  , Endang Purbaningrum
Published: January 2021

Effective learning using information technology is one of the opportunities that can be utilized by teachers to improve the effectiveness of learning. This study aims to describe the implementation of IT-based learning media creation training to improve teacher pedagogical skills as measured from the practical aspects and effectiveness of the Model used, namely the Critical Event Model (CEM). The subjects of this study were the teachers of SD Santo Yosef Surabaya. This research is an implementation research with quantitative approach. The data collection method used in this research is in the form of questionnaires to assess aspects of practicality and media assessment to assess aspects of effectiveness. The results showed that the training that had been evaluated through media assessment by the trainer and questionnaire scores by participants, both had a high percentage. Based on the results of the research shows that the implementation of training on creating IT-based learning media has been effective and practical
Development Of e Module Based Blended Learning On Hadith Material About Intention In Madrasah Ibtidaiyah
Authors: Dani Cahyani Rahayu  , Abdulloh Hamid  , Mohammad Salehuddin  , Wahyudi Winarto Eka
Published: January 2021

This study aims to develop a blended learning-based e modulon the hadith material about intentions at the madrasah ibtidaiyah or elementary education level. This study uses the research and development method (Research and Development) with the ADDIE or Analyze development model, Design, Development, Implementation, and Evaluate. The moduldeveloped is made using the Kvisoft Flipbook Maker application and published in Html format, which can be accessed via the internet network. The developed blended learningcombines face-to-face learning and distance learning as the learning process occurs, especially during the pandemic and the new normal. E modulis used as a companion to teaching materials that can stimulate students to study independently due to the parents' busyness or the teacher's distance, which makes the obligation to study independently echoed together with the independent campaign in learning. The validity and response tests carried out 85% agreed with the use of e moduland the pretest and posttest test of students' learning outcomes also showed the effectiveness of using the blended learning-based e modulwith an average of 87 out of 20 students who became respondents.
Viroterapia oncolítica aplicada ao tratamento do câncer em cães
Authors: Rocha Jorge Alberto Manso Raimundo da , Rios Fernanda Teodoro , Campos Marcello Fiqueredo , Souza Yânca Bizerra
Published: January 2021

O câncer continua a ser uma das principais causas de mortes em humanos e em cães. Os tumores de ocorrência natural em cães têm muitas similaridades biológicas com os cânceres humanos e respondem similarmente às terapias convencionais. Por isso, pode-se inferir que os protocolos clínicos adotados para os humanos poderiam ser transferidos para o tratamento de cães com câncer e que terapêuticas com êxito no tratamento de tais animais  poderiam servir para o tratamento de seres humanos. A viroterapia oncolítica em cães é o objeto deste artigo de revisão.
El problema campesino en la Revolución Mexicana
Authors: Mroziewicz Robert
Published: January 2021

Abstract/description by editorial team (2020):

The article analyzes the Mexican revolution in relation to the situation of Mexico’s rural population. The campesinos (villagers) are identified as a major force behind the revolution. This is due to the social inequality and contrasts in pre-Revolution Mexican society as well as the “agrarian question” (presence of 10 million landless, migratory agricultural workers). The article describes the structure of land use in relation to ethnicity and occupation. It also presents an historical overview of the “agrarian reform” in the course of the Mexican revolution. The article concludes that thr end results of land use reforms present a form of social agreement between two social groups active in the revolution: bourgeoisie and peasantry.
Sosialisasi Perpajakan, Tingkat Pemahaman, Kesadaran Wajib Pajak dan E-System Terhadap Kepatuhan Membayar PBB
Authors: Kemalaningrum Inneke Tirta , Octaviani Andri
Published: January 2021

Tujuan dari penelitian ini untuk memberikan bukti empiris mengenai pengaruh sosialisasi perpajakan, tingkat pemahaman, kesadaran wajib pajak, dan e-system terhadap kepatuhan wajib pajak dalam membayar pajak bumi dan bangunan di Kelurahan Kerten. Populasi dalam penelitian ini masyarakat wajib pajak bumi dan bangunan di Kelurahan Kerten, Laweyan, Kota Surakarta sejumlah 1.946 wajib pajak. Sampel penelitian ini sebanyak 100 wajib pajak yang berada di Kelurahan Kerten dengan teknik pengambilan sampel yang digunakan simple random sampling. Metode analisis yang digunakan analisis regresi linier berganda. Hasil penelitian menunjukkan bahwa sosialisasi perpajakan, tingkat pemahaman, kesadaran wajib pajak dan e-system berpengaruh positif dan signifikan terhadap kepatuhan wajib pajak dalam membayar pajak bumi dan bangunan di Kelurahan Kerten. Uji F menunjukkan bahwa sosialisasi perpajakan, tingkat pemahaman, kesadaran wajib pajak, dan e-system secara simultan berpengaruh positif dan signifikan terhadap kepatuhan wajib pajak dalam membayar pajak bumi dan bangunan di Kelurahan Kerten. Koefisien Determinasi (R2) menunjukkan bahwa variabel sosialisasi perpajakan, tingkat pemahaman, kesadaran wajib pajak, dan e-system berpengaruh sebesar 76,3% sedangkan sisanya 23,7% dipengaruhi oleh variabel lain yang tidak dijelaskan dalam penelitian ini.
PERBANDINGAN PERENCANAAN PERKERASAN JALAN RIGID PAVEMENT DENGAN MENGGUNAKAN METODE SNI PD T-14-2003 DAN NAASRA
Authors: Sumina  , Priyanto Kusdiman Joko
Published: January 2021

Perkerasan jalan adalah unsur konstruksi jalan raya yang sangat penting dalam rangka kelancaran transportasi darat sehingga memberikan kenyamanan dan keamanan bagi penggunanya, sehingga perlu direncanakan dengan baik berdasarkan standar dan kriteria perencanaan yang berlaku di Indonesia. Kurangnya tingkat pelayanan jalan mendesak segera dilakukan tahapan perencanaan pada setiap desain pembuatan jalan, karena tahapan tersebut sangat peranan penting dalam merencanakan tebal perkerasan yang baik khususnya pada perkerasan kaku, serta menurut data survey di ruas jalan tersebut. Penentuan nilai rancang tebal perkerasan kaku (Rigid Pavement) dapat dihitung dengan banyak metode, dalam perencanaan tebal perkerasan kaku ruas Jalan Ringroad Utara Kota Surakarta pada digunakan metode SNI Pd T-14-2003 dan methode NAASRA (National Association of Australian State Road Authorities). Dalam metode SNI Pd T-14-2003 didapatkan JSKN sebanyak 9 x 107 dan CBR tahan dasar 4.30 %, diperoleh ketebalan pelat setebal 180 mm. Sedangkan perencanaan metode NAASRA, didapat JSKN (Jumlah Sumbu Kendaraan Niaga) sebesar 2.07 x 108, nilai CBR tanah dasar 4,30% dan nilai K = 35kPa/mm, Tebal perkerasan   setebal 210 mm. Perencanaan penulangan dengan metode SNI Pd T-14-2003 adalah diameter 10 mm dengan jarak 250 mm.  Untuk metode NAASRAdiperoleh tulangan dengan diameter 12 mm jarak 250 mm. Untuk dimensi drainase pada lokasi penampang 1 adalah dengan lebar x tinggi (0.30 m x 0.30 m) dan untuk tinggi jagaan sebesar 0.1 m. Dan dimensi drainase pada lokasi penampang 2 adalah dengan lebar x tinggi (0.35 m x 0.35 m) dan untuk tinggi jagaan sebesar 0.1 m. Kecilnya dimensi saluran ini dipengaruhi oleh kontur jalan, yaitu jalan melewati daerah naik turun, dengan kemiringan > 5%.

Kata kunci: Rigid Pavement, Methode SNI Pd T-14-2003, Methode NAASRA
PENELITIAN STRATEGI PENGUATAN KERJASAMA KEBIJAKAN PEMBANGUNAN PARIWISATA DI JAWA TENGAH (STUDI KASUS KSPN DIENG DAN SEKITARNYA)
Authors: Risandewi Tri , Widiastuti Wiwin
Published: January 2021

Kawasan pariwisata Dieng merupakan kawasan wisata potensial di Jawa Tengah baik sumber daya alam, dan budayanya, namun menyimpan potensi konflik yang rentan. Sebagai suatu kawasan wisata yang potensial, keunikan Dieng tidak hanya karena adanya objek wisata yang beraneka ragam dalam satu kawasan, namun juga merupakan sebuah kawasan wisata yang terbagi menjadi dua wilayah administratif, yaitu Kabupaten Banjarnegara dan Kabupaten Wonosobo. Pengelolaan Kawasan Wisata Dieng yang baik akan sulit dilakukan tanpa adanya kerjasama antar daerah. Tujuan penelitian ini adalah untuk: 1) mengidentifikasi kondisi kerjasama antar daerah dalam pengelolaan KSPN Dieng dan sekitarnya; 2) menganalisis faktor-faktor penghambat dan pendorong kerjasama antar daerah; dan 3) merumuskan strategi penguatan kerjasama kebijakan pembangunan pariwisata di KSPN Dieng. Metode yang digunakan dalam penelitian ini adalah deksriptif kualitatif dengan lokasi enelitian di KabupatenWonosobo, Banjarnegara, Batang dan Pekalongan. Adapun hasil penelitian yang diperoleh yaitu (1) kerjasama antar daerah dalam hal pengelolaan KSPN Dieng sudah ada sejak tahun 1974 hingga tahun 2012, namun kerjasama dilakukan secara parsial, yaitu antar 2 (dua) kabupaten atau kerjasama pemerintah provinsi dengan 1 (satu) kabupaten dalam sub sektor pariwisata tertentu; (2) faktor-faktor yang menghambat kerjasama antar daerah diantaranya tidak ada persamaan persepsi, lemahnya koordinasi antar sektor,belum adanya regulasi kerjasama antar stakeholder, sedangkan faktor pendorong: dukungan stakeholder dan dana, serta banyaknya event wisata bersama; (3) strategi penguatan yang perlu dilakukan yaitu tata kelola destinasi pariwisata yang terpadu dan bersinergi dengan membentuk kelembagaan khusus.
PENGARUH PEMBERIAN POLLARD TEROLAH TERHADAP PERTUMBUHAN TOTAL BAKTERI ASAM LAKTAT DAN JAMUR PADA USUS HALUS AYAM KAMPUNG UMUR 7 MINGGU
Authors: Sulistiyanto B. , Utama C. S. , Ulfah M. R.
Published: January 2021

Penelitian ini dilakukan untuk mengkaji pengaruh pollard terolah terhadap jumlah bakteri asam laktat dan total jamur pada usus ayam kampung umur 7 minggu. Bahan yang digunakan adalah 200-an day old chick (DOC) ayam kampung asli dengan berat awal 38 ± 0,32g, pakan pabrik BR-IAJ, jagung, bungkil kedelai, vitamin-campuran, pollard, asam amino, limbah kubis yang difermentasi sebagai starter, sirup gula dan garam. Desain eksperimental yang digunakan adalah rancangan acak lengkap dengan 4 perlakuan dan 5 ulangan. Perlakuan terdiri dari pakan pabrik BR-IAJ sebagai kontrol positif (T0), pollard yang tidak diobati (T1), pollard yang diolah secara fisik (dikukus) (T2) dan pollard yang diolah secara fisik dan biologis (dikukus dan difermentasi) (T3). Hasil penelitian menunjukkan ayam yang diberi pakan berdasarkan pollard yang diproses secara fisik dan biologis lebih baik daripada kontrol, pada parameter jumlah bakteri asam laktat (BAL) di jejunum (p <0,05), sedangkan duodenum dan ileum memiliki tidak ada efek signifikan, begitu juga parameter jamur total tidak menunjukkan efek signifikan pada semua bagian usus (p> 0,05). Disimpulkan bahwa ransum olahan pollard (pengukusan dan fermentasi) berbasis lebih baik daripada pakan pabrik seperti yang terlihat pada aspek total bakteri asam laktat dalam usus ayam umur tujuh minggu.
BIOCULTURAL E A SUSTENTABILIDADE LOCAL NOS AGROECOSSISTEMAS AMAZÔNICOS DE TAQUARUÇU GRANDE, PALMAS-TO
Authors: Dos Santos Eliane Marques , Fraxe Therezinha de Jesus Pinto , De Athayde Simone Ferreira , Souza Lucas Barbosa e
Published: January 2021

Caracterizar as paisagens amazônicas de Taquaruçu Grande em Palmas-TO, a partir dos elementos bioculturais existentes, seus usos e a perspectiva de sustentabilidade local foram os objetivos deste artigo. A abordagem sistêmica consiste no eixo condutor para a coleta e análise dos dados com uso de observação sistemática, entrevista semiestruturada, registro de imagens e reuniões de grupo focais. Os resultados indicaram paisagens com valor histórico significativo por ainda exibir mosaicos complexos com vegetação em área de unidade de conservação, associadas à agrodiversidade, recursos hídricos e saberes locais. Essa complexidade deve-se à diversidade biocultural resultante de uma persistência histórica local que assinalam a resiliência para esses sistemas e por isso sua sustentabilidade nessas condições de uso.
A IMPORTÂNCIA DA MULHER NA AGRICULTURA FAMILIAR: COMUNIDADE LAGO JANAUACÁ, CAREIRO CASTANHO – AM
Authors: Erazo Rafael de Lima , Costa Sarah Caroline Ferreira das Chagas , Silva Lindomar de Jesus de Sousa
Published: January 2021

A Amazônia é, seguramente, uma região onde a agricultura familiar se manifesta das mais diferentes formas: ribeirinha, extrativista, quilombolas, dentre outros, e onde se encontra uma grande diversidade de recursos naturais capazes de prover a base produtiva e alimentar desses agricultores. O aumento do interesse pelas discussões sobre a importância social e econômica dessa unidade produtiva se materializou em políticas públicas, como o Programa Nacional de Fortalecimento da Agricultura Familiar (PRONAF), além do revigoramento da Reforma Agrária, destinadas a promover a melhoria da condição de vida deste segmento. A importância desse estudo assenta-se na necessidade de melhor conhecer a realidade da mulher na agricultura familiar no contexto amazônico e a sua importância para a sociedade contemporânea. É necessário que haja uma melhor compreensão da dinâmica interna das explorações familiares e do grau de sua integração ao sistema econômico e social vigente, através da análise da situação atual da Comunidade Lago Janauacá, promovendo uma discussão sobre a real igualdade entre os gêneros. O Objetivo desse trabalho é apresentar uma discussão a respeito do papel da mulher na agricultura familiar da região Amazônica. Os objetos que compuseram o universo da pesquisa foram as unidades familiares de produção associadas aos circuitos de produção e comercialização das “casas de farinha” flutuantes da região do lago Janauacá, município de Careiro, no Estado do Amazonas. Com esse estudo, pode-se verificar que na Amazônia, a mulher tem apresentado crescimento expressivo das atividades no campo. Porém, esse estudo ainda mostrou o retrato brasileiro da escolaridade da mulher que sobrevive do trabalho no campo. São necessárias políticas públicas que garantam o acesso à terra para a mulher, que proporcionem educação, cultura e lazer, que auxiliem na geração de emprego e na melhoria da renda das famílias que vivem da agricultura familiar no Amazonas. E principalmente, a valorização e a quantificação do trabalho das mulheres tenham reconhecimento, pois este contribui de maneira significativa para a sobrevivência e para a reprodução da família.
UM BREVE PANORAMA DA BIOPROSPECÇÃO: SUA ORIGEM, SUAS DEFINIÇÕES, POTENCIAL ECONÔMICO E STATUS-QUO NO BRASIL
Authors: Fraxe Therezinha de Jesus Pinto , Silva Michele Lins Aracaty e
Published: January 2021

A Sustentabilidade é um dos temas mais debatidos no mundo na era do século XXI, e, no caso do Brasil, tudo fica muito mais frágil, visto que nos primeiros dias da nova gestão, os representantes do Governo Federal alteraram as atribuições formais dos ministérios, produzindo a mais drástica reestruturação dos órgãos de primeiro escalão desde o governo Collor (1990-1992). A conclusão é de que as políticas socioambientais brasileiras, construídas em 40 anos de avanços, foram colocadas em xeque. Assim, pode-se dizer que a Bioprospecção é uma das ferramentas de rentabilidade e sustentabilidade mais eficiente que existe e dada a importância do assunto, ela será explicada neste trabalho bem como se demonstrará seu estado atual no Brasil.