bluuebunny/crossref_metadata_2025 huggingface dataset:
Experimental Simulation of Lightning Current Discharge on Rocks
Authors: Elmi Chiara , Coleman Nicholas S. , Miu Karen , Schruba Edward
Published: November 2018

Lightning is a transient, high-current discharge occurring within a thundercloud, between clouds, or between a cloud and the ground. Cloud-to-ground (CG) lightning is the most studied because of its impact on human life. The aim of this study is to elucidate the effects of lightning in Earth materials by simulating the lightning current discharges in a laboratory setting. Technical applications of this work include the study or development of customized materials used to prevent accidents, limit damage, or reduce interruptions in electrical power system owing to lightning strikes, such as lightning arresters or high-voltage fuses. High-voltage electrical arcs were discharged through rock specimens, and power, energy, and duration of discharge were estimated to provide a better understanding of the origin of naturally occurring fulgurites (shock-impact glasses) and the lightning/rock interaction. X-ray powder diffraction showed that the samples used for the experiment represent basalt (samples A0, A1–A4) and granite (samples B1, B2). Optical microscopy provides direct evidence that materials can be physically altered due to the heat generated by an arcing event. Optical microscopy observations showed that arcs passed through the target rocks and mimicked the effect of lightning strikes hitting the surface of the rock, melting the target rock, and passing to ground. Fulgurite glass observed on basalt samples shows the impact origin lining the surface of millimeter-size craters and a slash-like coating, whereas in the granite sample, the fulgurite was not observed because the arc passed directly to the laboratory ground. Significant differences in the duration of the experimental electrical arcs that passed through dry and wet samples (A1 and A3; A2 and A4, respectively) were observed. This discrepancy can be ascribed to the variation of the electrical properties related to the distribution of the water layer on the rock sample and to the occurrence of magnetite grains, which may increase the local conductivity of the sample owing to its electromagnetic properties.
Improvement of the Impact Properties of Composite Laminates by Means of Nano-Modification of the Matrix—A Review
Authors: Saghafi Hamed , Fotouhi Mohamad , Minak Giangiacomo
Published: November 2018

This paper reviews recent works on the application of nanofibers and nanoparticle reinforcements to enhance the interlaminar fracture toughness, to reduce the impact induced damage and to improve the compression after impact performance of fiber reinforced composites with brittle thermosetting resins. The nanofibers have been mainly used as mats embedded between plies of laminated composites, whereas the nanoparticles have been used in 0D, 1D, 2D, and 3D dimensional patterns to reinforce the matrix and consequently the composite. The reinforcement mechanisms are presented, and a comparison is done between the different papers in the literature. This review shows that in order to have an efficient reinforcement effect, careful consideration is required in the manufacturing, materials selection and reinforcement content and percentage. The selection of the right parameters can provide a tough and impact resistant composite with cost effective reinforcements.
Microwave Spectroscopic Detection of Human Hsp70 Protein on Annealed Gold Nanostructures on ITO Glass Strips
Authors: Ionescu Rodica Elena , Selon Raphael , Pocholle Nicolas , Zhou Lan , Rumyantseva Anna , Bourillot Eric , Lesniewska Eric
Published: November 2018

Conductive indium-tin oxide (ITO) and non-conductive glass substrates were successfully modified with embedded gold nanoparticles (AuNPs) formed by controlled thermal annealing at 550 °C for 8 h in a preselected oven. The authors characterized the formation of AuNPs using two microscopic techniques: scanning electron microscopy (SEM) and atomic force microscopy (AFM). The analytical performances of the nanostructured-glasses were compared regarding biosensing of Hsp70, an ATP-driven molecular chaperone. In this work, the human heat-shock protein (Hsp70), was chosen as a model biomarker of body stress disorders for microwave spectroscopic investigations. It was found that microwave screening at 4 GHz allowed for the first time the detection of 12 ng/µL/cm2 of Hsp70.
Student Perceptions of Motor, Mental and Social Benefits and the Impact of Practicing Recreational Figure Skating
Authors: Ionescu Anca , Badau Dana
Published: November 2018

The study aims to highlight the impact of practicing recreational figure skating by evaluating the relationship between the perceptions of motor, mental and social benefits of practicing figure skating and the frequency and duration allocated for this recreational activity. The study included 143 students of physical education and sport specialization. The questionnaire was designed to evaluate the students’ perception about the benefits of practicing recreational figure skating; it included 30 items divided in three parts: motor, mental and social benefits. Each of them contained 10 items to be assessed by students using the Likert scale, with 2 items related to the duration and frequency of practicing figure skating during recreational time. The results were processed using SPPS 24. The results were statistically significant at p < 0.05. The Cronbach’s Alpha coefficient of the questionnaire was α = 0.965, suggesting that the items had high internal consistency. Using the Likert scale, we found the following: concerning the high score 5 points—the motor capacity 62.9%, the mental capacity 49.7%, the social capacity 49.7%; and for a low score of 1 point—mental and social capacity 1.4%, motor capacity 0.7%. The effect size was medium for almost all items. No statistically significant correlations were found between the result of the questionnaire and the frequency and duration of practicing figure skating during free time. Figure skating is considered by students to be one of the activities through which a series of parameters of physical development and level of physical fitness can be improved through the expansion of motor skills. Also, the practice of figure skating contributes to the formation of proactive behaviors by improving the mental and social abilities of practitioners.
Optimal Thermal Characteristics of the Courtyard in the Hot and Arid Climate of Isfahan
Authors: Hasehzadeh Haseh Roya , Khakzand Mehdi , Ojaghlou Morteza
Published: November 2018

The courtyard is a common and traditional architectural concept in Middle Eastern countries, especially Iran. This study presents practical solutions to arrive at the optimal form and features of a courtyard based on this traditional architecture to cope with the hot and arid climate of Isfahan, Iran. A model using the ENVI-met 3.1 software was used to simulate the courtyard orientation, the height of the wall enclosure, the albedo of the materials and the vegetation level in Isfahan. As the first step of the simulation, different orientations were simulated. Based on the results, the northern courtyard is recommended as the optimum orientation for the remainder of the simulation process. A high wall enclosure is recommended in the second step of simulation to ensure cool temperatures at noon and warmth at night. In the third step, it is advised to use low-albedo material in the courtyards to avoid the extra reflected radiation of used materials on the inhabitants. The final step suggests that more than 50% of the courtyard plan should be covered by greenery to ensure thermal comfort in the courtyards. Based on these suggestions, designers can create more sustainable courtyards that are better suited to the hot and arid climate of Isfahan.
Effect of PTFE Particle Size on Superhydrophobic Coating for Supercooled Water Prevention
Authors: Morita Katsuaki , Gonzales Joseph , Sakaue Hirotaka
Published: November 2018

Polytetrafluoroethylene (PTFE) chemically repels water droplets due to the nature of fluorine substituents. This paper presents an experimental study on the impact of PTFE particle size and temperature on the hydrophobicity of a surface. The present study analyzes hydrophobicity due to both the chemical properties of PTFE and the microstructure created by PTFE particles. Herein, studies of the contact angle and the sliding angle of these surfaces are described in supercooled-water conditions ranging from −10 to 0 °C. From the equations governing the surface tension and sliding angle of a droplet on a superhydrophobic surface, it is found that particle size has a much greater effect on hydrophobicity than temperature. An increase in the PTFE particle size greatly reduces the sliding angle, which indicates a lower amount of energy required to remove the droplet from the surface.
Adaptations by Zostera marina Dominated Seagrass Meadows in Response to Water Quality and Climate Forcing
Authors: Shields Erin C. , Moore Kenneth A. , Parrish David B.
Published: November 2018

Global assessments of seagrass declines have documented accelerating rates of loss due to anthropogenic sediment and nutrient loadings, resulting in poor water quality. More recently, global temperature increases have emerged as additional major stressors. Seagrass changes in the Chesapeake Bay, USA provide important examples of not only the effects of human disturbance and climate forcing on seagrass loss, but also meadow recovery and resiliency. In the York River sub-tributary of the Chesapeake Bay, the meadows have been monitored intensively using annual aerial imagery, monthly transect surveys, and continuous water quality measurements. Here, Zostera marina has been demonstrating a shift in its historical growth patterns, with its biomass peaking earlier in the growing season and summer declines beginning earlier. We found an increasing trend in the length of the most stressful high temperature summer period, increasing by 22 days since 1950. Over the past 20 years, Z. marina’s abundance has exhibited periods of decline followed by recovery, with recovery years associated with greater spring water clarity and less time spent at water temperatures > 28 °C. Although human disturbance and climatic factors have been altering these seagrass meadows, resilience has been evident by an increase in reproductive output and regrowth from Z. marina seedlings following declines, as well as expansions of Ruppia maritima into areas previously dominated by Z. marina.
Derating Guidelines for Lithium-Ion Batteries
Authors: Sun Yongquan , Saxena Saurabh , Pecht Michael
Published: November 2018

Derating is widely applied to electronic components and products to ensure or extend their operational life for the targeted application. However, there are currently no derating guidelines for Li-ion batteries. This paper presents derating methodology and guidelines for Li-ion batteries using temperature, discharge C-rate, charge C-rate, charge cut-off current, charge cut-off voltage, and state of charge (SOC) stress factors to reduce the rate of capacity loss and extend battery calendar life and cycle life. Experimental battery degradation data from our testing and the literature have been reviewed to demonstrate the role of stress factors in battery degradation and derating for two widely used Li-ion batteries: graphite/LiCoO2 (LCO) and graphite/LiFePO4 (LFP). Derating factors have been computed based on the battery capacity loss to quantitatively evaluate the derating effects of the stress factors and identify the significant factors for battery derating.
The Role of Demand Response Aggregators and the Effect of GenCos Strategic Bidding on the Flexibility of Demand
Authors: Mohammad Nur , Mishra Yateendra
Published: November 2018

This paper presents an interactive trading decision between an electricity market operator, generation companies (GenCos), and the aggregators having demand response (DR) capable loads. Decisions are made hierarchically. At the upper-level, an electricity market operator (EMO) aims to minimise generation supply cost considering a DR transaction cost, which is essentially the cost of load curtailment. A DR exchange operator aims to minimise this transaction cost upon receiving the DR offer from the multiple aggregators at the lower level. The solution at this level determines the optimal DR amount and the load curtailment price. The DR considers the end-user’s willingness to reduce demand. Lagrangian duality theory is used to solve the bi-level optimisation. The usefulness of the proposed market model is demonstrated on interconnection of the Pennsylvania-New Jersey-Maryland (PJM) 5-Bus benchmark power system model under several plausible cases. It is found that the peak electricity price and grid-wise operation expenses under this DR trading scheme are reduced.
Improvements in Bidirectional Power-Flow Balancing and Electric Power Quality of a Microgrid with Unbalanced Distributed Generators and Loads by Using Shunt Compensators
Authors: Chang Wei-Neng , Chang Chia-Min , Yen Shao-Kang
Published: November 2018

Improper connections of unbalanced distributed generators (DGs) and loads in a three-phase microgrid cause unbalanced and bidirectional power flow problems. The unbalanced DGs and loads may also aggravate the electric power quality (EPQ), such as voltage regulation, power factor, and unbalanced current and voltage. This increases the difficulty of operation in a microgrid. In this study, a three-phase, delta-connected, shunt-type universal compensator was employed for achieving the bidirectional power-flow balancing and improving the EPQ of a three-phase, distribution-level microgrid with unbalanced DGs and loads. A feedforward compensation scheme was derived for the compensator by using the symmetrical components method. In practical applications, the universal compensator can be implemented as static var compensators (SVCs), static synchronous compensators (STATCOMs), or an additional function of active filters. With the on-line compensation of the proposed compensator, the bidirectional power-flow balancing and EPQ improvement in the microgrid were achieved. A demonstration system was proposed to present the effectiveness of the compensator.
A Novel Control Strategy for Grid-Connected Inverter Based on Iterative Calculation of Structural Parameters
Authors: Sun Zhenao , Wang Dazhi , Yuan Tianqing , Liu Zairan , Yu Jiahui
Published: November 2018

A novel control strategy that is based on iterative calculation of structural parameters is proposed for grid-connected inverter in this paper. The proposed strategy has a good dynamic performance, which makes it particularly suitable for the application of PV grid-connected generation. First, a second-order discretization mathematical model of grid-connected inverter control is established in the dq frame. The corresponding relation between the control signal and the output current is deduced in formulas. Then, the values of structural parameters in the formulas can be obtained through iterative calculation, which can further reduce the amount of calculation. After several iteration cycles, the structural parameters are approximately equal to their actual values and the inverter can be controlled as an open-loop system with its dynamic performance optimized. At last, simulation and experiments are performed. The results show that the static performance of the proposed strategy is as good as that of the classical ones, but its dynamic performance is improved significantly.
Impact of Holder Materials on the Heating and Explosive Breakup of Two-Component Droplets
Authors: Antonov Dmitry , Bellettre Jérôme , Tarlet Dominique , Massoli Patrizio , Vysokomornaya Olga , Piskunov Maxim
Published: November 2018

The heating of two-component droplets and the following explosive breakup of those droplets have been extensively studied over the most recent years. These processes are of high interest, since they can significantly improve the performance of many technologies in fuel ignition, thermal and flame liquid treatment, heat carriers based on flue gases, vapors and water droplets, etc. Research throughout the world involves various schemes of droplet heating and supply (or, less frequently, injection) to heating chambers. The most popular scheme features the introduction of a two-component or multi-component droplet onto a holder into the heating chamber. In this research, we study how holder materials affect the conditions and integral characteristics of droplet heating and explosive breakup: heating time until boiling temperature; minimum temperature sufficient for droplet breakup; number and size of fragments in the resulting droplet aerosol, etc. Experiments involve droplets that are produced from flammable (oil) and non-flammable (water) components with significantly different thermophysical and optical properties, as well as boiling temperature and heat of vaporization. The most popular elements with the scientific community, such as ceramic, steel, aluminum, copper, and phosphorus rods, as well as a nichrome wire, serve as holders. We establish the roles of energy inflow from a holder to a droplet, and energy outflow in the opposite direction. We compare the holder results with a supporting thermocouple, recording the drop temperature under a heat transfer provided at 350°C. Finally, we forecast the conditions that are required for a significant improvement in the performance of thermal and flame water treatment through the explosive breakup of two-component droplets.
Peer-to-Peer Energy Trading among Microgrids with Multidimensional Willingness
Authors: Wang Ning , Xu Weisheng , Xu Zhiyu , Shao Weihui
Published: November 2018

Networked microgrids are emerging for coordinating distributed energy resources in distribution networks in the future Energy Internet, for which developing an efficient energy market model is crucial for facilitating multi-directional trading among microgrids. In this paper, a peer-to-peer energy trading mechanism is presented using non-cooperative bidding among microgrids. Multidimensional willingness, including time pressure and counter behavior for mimicking the personalized behaviors of microgrids, was taken into account in the design of the bidding strategy. Under a parallel trading framework based on a blockchain, the proposed multidimensional willingness bidding strategy turns out to be able to make rational decisions with sufficient flexibility in the bidding process. The simulation results of a realistic case of microgrids from Guizhou Province, China, validate that the proposed peer-to-peer energy trading mechanism is capable of raising the microgrids’ profits and renewable energy source utilization.
An Integration Mechanism between Demand and Supply Side Management of Electricity Markets
Authors: Liu Zixu , Zeng Xiaojun , Meng Fanlin
Published: November 2018

One of the main challenges in the emerging smart grid is to jointly consider the demand and supply, which is also reflected in the wholesale market (supply side) and the retail market (demand side). When integrating the demand and supply side into one framework, the mechanism for determining the market clearing price has been changed. This is due to the demand variations in the demand side in response to the market clearing price and the change of generation costs in the supply side from the demand variation. In order to find the best balance between the supply and demand under the demand response management scheme, this paper proposes a new integrated supply and demand coordination mechanism for the electricity market and smart pricing methods for generator and retailers. Another important contribution of this paper is to develop an efficient algorithm to find the match equilibrium between the demand and supply sides in the new proposed mechanism. Experimental results demonstrate that the new mechanism can effectively handle unpredictable demand under dynamic retail pricing and support the ISO to dispatch the generation economically. It can also help in achieving the goals of dynamic pricing such as maximizing the profits for retailers.
Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress
Authors: Saelao Perot , Wang Ying , Chanthavixay Ganrea , Yu Vivian , Gallardo Rodrigo A. , Dekkers Jack C. M. , Lamont Susan J. , Kelly Terra , Zhou Huaijun
Published: November 2018

Newcastle disease virus (NDV) is a devastating worldwide poultry pathogen with major implications for global food security. In this study, two highly inbred and genetically distinct chicken lines, Fayoumis and Leghorns, were exposed to a lentogenic strain of NDV, while under the effects of heat stress, in order to understand the genetic mechanisms of resistance during high ambient temperatures. Fayoumis, which are relatively more resistant to pathogens than Leghorns, had larger numbers of differentially expressed genes (DEGs) during the early stages of infection when compared to Leghorns and subsequently down-regulated their immune response at the latter stages to return to homeostasis. Leghorns had very few DEGs across all observed time points, with the majority of DEGs involved with metabolic and glucose-related functions. Proteomic analysis corroborates findings made within Leghorns, while also identifying interesting candidate genes missed by expression profiling. Poor correlation between changes observed in the proteomic and transcriptomic datasets highlights the potential importance of integrative approaches to understand the mechanisms of disease response. Overall, this study provides novel insights into global protein and expression profiles of these two genetic lines, and provides potential genetic targets involved with NDV resistance during heat stress in poultry.
Common Chromosomal Fragile Sites—Conserved Failure Stories
Authors: Voutsinos Vasileios , Munk Sebastian H. N. , Oestergaard Vibe H.
Published: November 2018

In order to pass on an intact copy of the genome during cell division, complete and faithful DNA replication is crucial. Yet, certain areas of the genome are intrinsically challenging to replicate, which manifests as high local mutation propensity. Such regions include trinucleotide repeat sequences, common chromosomal fragile sites (CFSs), and early replicating fragile sites (ERFSs). Despite their genomic instability CFSs are conserved, suggesting that they have a biological function. To shed light on the potential function of CFSs, this review summarizes the similarities and differences of the regions that challenge DNA replication with main focus on CFSs. Moreover, we review the mechanisms that operate when CFSs fail to complete replication before entry into mitosis. Finally, evolutionary perspectives and potential physiological roles of CFSs are discussed with emphasis on their potential role in neurogenesis.
Contributions to Coastal Flooding Events in Southeast of Vietnam and their link with Global Mean Sea Level Rise
Authors: Melo de Almeida Luis Pedro , Almar Rafael , Meyssignac Benoit , Viet Nguyen Trung
Published: November 2018

This work analyzes the components of the total water level (TWL) that cause flooding in a tropical coastal area (Nha Trang beach, Southeast of Vietnam), and examines their link with global mean sea level rise (GMSLR). Interactions between the wave induced run-up (R) and astronomical tide (AT) were responsible for 43% of the 35 flooding events identified between 1993 and 2015. Most of these events (97%) took place during the winter monsoon season, when long-lasting extreme R and positive non-tidal residual (NTR) are likely to occur. Removal of the GMSLR trend from the NTR was found to affect the flood occurrence of 17% of these events, while the trend in wave height did not have any detectable impact. Our research highlights the direct connection between global climate changes and coastal flooding events.
Imaging and Characterization of Extrasolar Planets with the Next Generation of Space Telescopes
Authors: Gómez de Castro Ana I.
Published: November 2018

The study and characterization of the exoplanets’ atmospheres and composition is in its infancy. The large facilities that will make feasible to image an exo-Earth are currently under study. This contribution to the special issue on “detection and characterization of extrasolar planets” is a summary on the current status of the design studies to build large space-based facilities working in the 100–3000 nm range for this purpose. The three basic designs: Fresnel imagers, starshades, and coronagraphs on large space telescopes are described. An outline of the pros and cons for each design is provided. The relevance of transmission spectroscopy to characterize exoplanets atmospheres is pointed out.
Comment on Iavicoli et al. Ethics and Occupational Health in the Contemporary World of Work. Int. J. Environ. Res. Public Health 2018, 15, 1713
Authors: Yeh Ming-Jui , Liu Hsi-Chen
Published: November 2018

In their recent paper, Iavicoli and colleagues provided a narrative review on the historical development of ethics in occupational health and discussed several ethical concerns; they also hinted the “next step” for resolving the ethical challenges that occupational health professionals (OHPs) will encounter [...]
Treatment and Cost of Hepatocellular Carcinoma: A Population-Based Cohort Study in Taiwan
Authors: Nguang Seng-Howe , Wu Cheng-Kun , Liang Chih-Ming , Tai Wei-Chen , Yang Shih-Cheng , Ku Ming-Kun , Yuan Lan-Ting , Wang Jiunn-Wei , Tseng Kuo-Lun , Hung Tsung-Hsing , Hsu Pin-I , Wu Deng-Chyang , Chuah Seng-Kee , Hsu Chien-Ning
Published: November 2018

Hepatitis B virus vaccination and antiviral therapies reduce the risk of hepatocellular carcinoma (HCC). However, the lifetime healthcare expenditure involved in caring for HCC patients remains unclear. We examined the use and direct costs of healthcare services for a cohort of HCC patients to the healthcare system using Taiwan national health insurance program research database between 1997 and 2012. Total medical cost for all reimbursed patient encounters, including hospitalizations and outpatient care was cumulated from HCC onset to the end of follow-up or death. The mean follow-up time was 2.7 years (standard deviation, SD = 3.3) for the entire HCC cohort. Insurance payments of approximately US$92 million were made to 5522 HCC patients, with a mean cost of US$16,711 per patient (21,350). On average, the total cost per patient per month was US$2143 (5184); it was 50% higher for advanced cirrhosis patients at the baseline but 23% lower for mild-to-moderate cirrhotic patients. In the two-part regression, patients’ underlying comorbid conditions, liver transplants, hepatectomy, and transarterial chemoembolization were associated with increased total cost, with liver transplants having the greatest impact over time. Hepatocellular carcinoma imposes substantial burden on the healthcare system. Real-world evidence on treatment and cost outcomes highlighted the needs to expand effective screening strategies and to optimize healthcare delivery to meet HCC patients’ clinical needs.
Infections in Healthcare Workers in Germany—22-Year Time Trends
Authors: Nienhaus Albert
Published: November 2018

Health workers (HWs) run an increased risk of infection. The standardised data set of an accident insurer was used to analyse the time trends of infection-related claims and confirmed occupational diseases (ODs) in HWs. The numbers of claims and confirmed claims for different infections were analysed for the years 1996 to 2017. The rate of claims and confirmed ODs were calculated per 100,000 full-time workers. The number of claims was relatively stable over time. However, the rate per 100,000 full-time workers decreased from 25.2 to 15.4. The decrease was most pronounced for hepatitis B and hepatitis C infections, which were the most frequent infections for which claims were made at the start of the period. In 2017, tuberculosis (TB)-related claims were more frequent than those related to blood-borne virus infections. However, the growing number of TB claims does not reflect an increased infection risk, but rather improved methods for the diagnosis of latent TB infection (LTBI). Measures to prevent blood-borne virus infections in HWs were successful in the last 22 years, but attention should be paid to newly emerging infections.
Strategies for Promoting the Medical Device Industry in Korea: An Analytical Hierarchy Process Analysis
Authors: Lee Munjae
Published: November 2018

This study examines the medical device industry in the context of the Fourth Industrial Revolution and identifies the key strategies and general directions for promoting this industry through analytical hierarchy process (AHP). It is based on discussions of the key issues with specialists such as doctors, medical device companies, hospital staff, professors, and government agencies in the medical device industry. A total of 18 responses were obtained from the survey, and an analysis was conducted on the results. Among the medical device strategies identified by the surveyed specialists, clarifying the application of regulations (access strategy), increasing R&D investment for the technological development of medical devices (expansion strategy), and increasing support for global market expansion (infrastructure expansion strategy) were found to have the highest priority. Based on the above, we suggest key strategies and directions for promoting the medical device industry.
Spatial Characteristics of Heavy Metals in Street Dust of Coal Railway Transportation Hubs: A Case Study in Yuanping, China
Authors: Li Dongyue , Liao Yilan
Published: November 2018

Coal is a vital basic energy source in China, and rail serving is its major mode of transportation. Heavy metals in street dust surrounding the coal railway do harm to the environment and pose a potential risk to human health. This paper aims to identify the effects of coal transportation hubs on heavy metals in street dust. The geoaccumulation index and ecological risk index were used to assess the contamination levels of the following elements in Yuanping, Shanxi: arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), mercury (Hg), nickel (Ni), lead (Pb), and zinc (Zn). The levels of contamination of these heavy metals in soils were compared to those in street dust, and the difference between the railway’s and mining’s impacts on dust’s heavy-metal concentrations was explored. The results indicated that Cr and Pb in street dust were mainly affected by coal railway transportation, and the interaction effect of coal railway transportation and mining was greater than either of them alone. A potential control and prevention zone for Cr and Pb extending 1 km to both sides of the railway was identified. This work proves that coal railway transportation has certain effect on heavy metals in street dust and provides a scientific approach for future environmental impact assessments of coal transportation via railway.
Doxing Victimization and Emotional Problems among Secondary School Students in Hong Kong
Authors: Chen Qiqi , Chan Ko Ling , Cheung Anne Shann Yue
Published: November 2018

Doxing is the searching for and intentional disclosure of private information about a particular individual on the Internet without his or her consent, and is often used to exact punishment. The aim of this study was to investigate the associations between doxing victimization and emotional problems in secondary school students, paying particular regard to the impacts of different types of doxed information, the relationship between the perpetrators and victims of doxing, and the nature of the online platforms where doxing occurs. A sample of 2120 Hong Kong secondary school students of differing socioeconomic backgrounds participated in the study. The results show that almost all types of disclosed personal information result in negative feelings in victims, including depression, anxiety, and stress. Girls were also found to be more likely than boys to be doxed. Significant associations were found between emotional problems and the disclosure of mobile phone numbers and personal photos and videos; doxing conducted by schoolmates and anxiety and depression, and doxing through Instant Messenger and anxiety. Further exploration of integrated cyber violence prevention programs and research on the details of doxing are recommended.
Complex Investigation of a Pediatric Haematological Case: Haemophagocytic Syndrome Associated with Visceral Leishmaniasis and Epstein–Barr (EBV) Co-Infection
Authors: Tascini Giorgia , Lanciotti Lucia , Sebastiani Lisa , Paglino Alessandra , Esposito Susanna
Published: November 2018

Background: Visceral leishmaniasis (VL) is an anthropozoonosis caused by an intracellular parasite belonging to the genus Leishmania. In the Mediterranean region, L. donovani and L. infantum are responsible for VL and dogs are the main reservoir. Haemophagocytic lymphohistiocytosis (HLH) represents a complication of VL and consists of unrestrained activation and proliferation of lymphocytes and macrophages, leading to uncontrolled immune activation. Haemophagocytic lymphohistiocytosis may also develop during viral infection, and Epstein–Barr virus (EBV) infection is one of the main HLH causes. Macrophage haemophagocytosis in the bone marrow aspirate is pathognomonic. Case presentation: The case involves a 19-month-old male infant presenting with a high persistent fever with a fluctuating pattern, pancytopaenia, hepatosplenomegaly, and a high triglyceride level. Initial investigations showed an EBV infection. Considering the persistent signs and symptoms, bone marrow aspiration was performed and confirmed the suspicion of HLH. In addition, the presence of Leishmania infection was shown. The patient was treated with liposomal amphotericin B and had complete resolution of his symptoms. Conclusion: Diagnosis of VL represents a demanding challenge in endemic and non-endemic areas. Our case demonstrates that leishmaniasis should always be considered in the differential diagnosis in patients presenting with hepatosplenomegaly and cytopaenia with a persistent fever, even in cases of infectious mononucleosis. Moreover, the execution of bone marrow aspiration should not be delayed in order to diagnose and treat at an early stage the potential occurrence of VL, especially if complicated with HLH.
Exploring Medical Expenditure Clustering and the Determinants of High-Cost Populations from the Family Perspective: A Population-Based Retrospective Study from Rural China
Authors: Lu Shan , Zhang Yan , Niu Yadong , Zhang Liang
Published: November 2018

The costliest 5% of the population (identified as the “high-cost” population) accounts for 50% of healthcare spending. Understanding the high-cost population in rural China from the family perspective is essential for health insurers, governments, and families. Using the health insurance database, we tallied 202,482 families that generated medical expenditure in 2014. The Lorentz curve and the Gini coefficient were adopted to describe the medical expenditure clustering, and a logistic regression model was used to identify the determinants of high-cost families. Household medical expenditure showed an extremely uneven distribution, with a Gini coefficient of 0.76. High-cost families spent 54.0% of the total expenditure. The values for family size, average age, and distance from and arrival time to the county hospital of high-cost families were 4.05, 43.18 years, 29.67 km, and 45.09 min, respectively, which differed from the values of the remaining families (3.68, 42.46 years, 30.47 km, and 46.29 min, respectively). More high-cost families live in towns with low-capacity township hospitals and better traffic conditions than the remaining families (28.98% vs. 12.99%, and 71.19% vs. 69.6%, respectively). The logistic regression model indicated that family size, average age, children, time to county hospital, capacity of township hospital, traffic conditions, economic status, healthcare utilizations, and the utilization level were associated with high household medical expenditure. Primary care and health insurance policy should be improved to guide the behaviors of rural residents, reduce their economic burden, and minimize healthcare spending.
Regenerative Models for the Integration and Regeneration of Head Skeletal Tissues
Authors: Vieira Warren A. , McCusker Catherine D.
Published: November 2018

Disease of, or trauma to, the human jaw account for thousands of reconstructive surgeries performed every year. One of the most popular and successful treatment options in this context involves the transplantation of bone tissue from a different anatomical region into the affected jaw. Although, this method has been largely successful, the integration of the new bone into the existing bone is often imperfect, and the integration of the host soft tissues with the transplanted bone can be inconsistent, resulting in impaired function. Unlike humans, several vertebrate species, including fish and amphibians, demonstrate remarkable regenerative capabilities in response to jaw injury. Therefore, with the objective of identifying biological targets to promote and engineer improved outcomes in the context of jaw reconstructive surgery, we explore, compare and contrast the natural mechanisms of endogenous jaw and limb repair and regeneration in regenerative model organisms. We focus on the role of different cell types as they contribute to the regenerating structure; how mature cells acquire plasticity in vivo; the role of positional information in pattern formation and tissue integration, and limitations to endogenous regenerative and repair mechanisms.
Traumatic Brain Injury in Aged Mice Induces Chronic Microglia Activation, Synapse Loss, and Complement-Dependent Memory Deficits
Authors: Krukowski Karen , Chou Austin , Feng Xi , Tiret Brice , Paladini Maria-Serena , Riparip Lara-Kirstie , Chaumeil Myriam M. , Lemere Cynthia , Rosi Susanna
Published: November 2018

Traumatic brain injury (TBI) is of particular concern for the aging community since there is both increased incidence of TBI and decreased functional recovery in this population. In addition, TBI is the strongest environmental risk factor for development of Alzheimer’s disease and other dementia-related neurodegenerative disorders. Critical changes that affect cognition take place over time following the initial insult. Our previous work identified immune system activation as a key contributor to cognitive deficits observed in aged animals. Using a focal contusion model in the current study, we demonstrate a brain lesion and cavitation formation, as well as prolonged blood–brain barrier breakdown. These changes were associated with a prolonged inflammatory response, characterized by increased microglial cell number and phagocytic activity 30 days post injury, corresponding to significant memory deficits. We next aimed to identify the injury-induced cellular and molecular changes that lead to chronic cognitive deficits in aged animals, and measured increases in complement initiation components C1q, C3, and CR3, which are known to regulate microglial–synapse interactions. Specifically, we found significant accumulation of C1q on synapses within the hippocampus, which was paralleled by synapse loss 30 days post injury. We used genetic and pharmacological approaches to determine the mechanistic role of complement initiation on cognitive loss in aging animals after TBI. Notably, both genetic and pharmacological blockade of the complement pathway prevented memory deficits in aged injured animals. Thus, therapeutically targeting early components of the complement cascade represents a significant avenue for possible clinical intervention following TBI in the aging population.
Functional Annotation of Bacterial Signal Transduction Systems: Progress and Challenges
Authors: Martín-Mora David , Fernández Matilde , Velando Félix , Ortega Álvaro , Gavira José A. , Matilla Miguel A. , Krell Tino
Published: November 2018

Bacteria possess a large number of signal transduction systems that sense and respond to different environmental cues. Most frequently these are transcriptional regulators, two-component systems and chemosensory pathways. A major bottleneck in the field of signal transduction is the lack of information on signal molecules that modulate the activity of the large majority of these systems. We review here the progress made in the functional annotation of sensor proteins using high-throughput ligand screening approaches of purified sensor proteins or individual ligand binding domains. In these assays, the alteration in protein thermal stability following ligand binding is monitored using Differential Scanning Fluorimetry. We illustrate on several examples how the identification of the sensor protein ligand has facilitated the elucidation of the molecular mechanism of the regulatory process. We will also discuss the use of virtual ligand screening approaches to identify sensor protein ligands. Both approaches have been successfully applied to functionally annotate a significant number of bacterial sensor proteins but can also be used to study proteins from other kingdoms. The major challenge consists in the study of sensor proteins that do not recognize signal molecules directly, but that are activated by signal molecule-loaded binding proteins.
I’ve Gut A Feeling: Microbiota Impacting the Conceptual and Experimental Perspectives of Personalized Medicine
Authors: Amedei Amedeo , Boem Federico
Published: November 2018

In recent years, the human microbiota has gained increasing relevance both in research and clinical fields. Increasing studies seem to suggest the centrality of the microbiota and its composition both in the development and maintenance of what we call “health” and in generating and/or favoring (those cases in which the microbiota’s complex relational architecture is dysregulated) the onset of pathological conditions. The complex relationships between the microbiota and human beings, which invest core notions of biomedicine such as “health” and “individual,” do concern not only problems of an empirical nature but seem to require the need to adopt new concepts and new perspectives in order to be properly analysed and utilized, especially for their therapeutic implementation. In this contribution we report and discuss some of the theoretical proposals and innovations (from the ecological component to the notion of polygenomic organism) aimed at producing this change of perspective. In conclusion, we summarily analyze what impact and what new challenges these new approaches might have on personalized/person centred/precision medicine.
Role of p38 MAPK in Atherosclerosis and Aortic Valve Sclerosis
Authors: Reustle Anna , Torzewski Michael
Published: November 2018

Atherosclerosis and aortic valve sclerosis are cardiovascular diseases with an increasing prevalence in western societies. Statins are widely applied in atherosclerosis therapy, whereas no pharmacological interventions are available for the treatment of aortic valve sclerosis. Therefore, valve replacement surgery to prevent acute heart failure is the only option for patients with severe aortic stenosis. Both atherosclerosis and aortic valve sclerosis are not simply the consequence of degenerative processes, but rather diseases driven by inflammatory processes in response to lipid-deposition in the blood vessel wall and the aortic valve, respectively. The p38 mitogen-activated protein kinase (MAPK) is involved in inflammatory signaling and activated in response to various intracellular and extracellular stimuli, including oxidative stress, cytokines, and growth factors, all of which are abundantly present in atherosclerotic and aortic valve sclerotic lesions. The responses generated by p38 MAPK signaling in different cell types present in the lesions are diverse and might support the progression of the diseases. This review summarizes experimental findings relating to p38 MAPK in atherosclerosis and aortic valve sclerosis and discusses potential functions of p38 MAPK in the diseases with the aim of clarifying its eligibility as a pharmacological target.
Apoptosis Induction of Agave lechuguilla Torrey Extract on Human Lung Adenocarcinoma Cells (SK-LU-1)
Authors: Anguiano-Sevilla Luis Alberto , Lugo-Cervantes Eugenia , Ordaz-Pichardo Cynthia , Rosas-Trigueros Jorge Luis , Jaramillo-Flores María Eugenia
Published: November 2018

In this study, an ethanol extract of Agave lechuguilla was evaluated against six carcinogenic cell lines (HCT-15, MCF-7, PC-3, U-251, SK-LU-1 and K-562) with an inhibition of 75.7 ± 2.3% against the SK-LU-1 line. Based on the previous result, the extract was hydrolyzed and fractionated, to which the IC50 was determined; the cell line was more sensitive to the fractionated extract with an IC50 6.96 ± 0.15 µg/mL. Characterization by mass spectrometry showed the presence of kaempferol, quercetin and a flavonoid dimer formed by afzelechin-4β-8-quercetin, according to the generated fragmentation pattern. The fractionated extract presented cell death by apoptosis with 39.8% at 24 h. Molecular docking was performed with the molecules found to try to describe cell death by apoptosis through death receptors such as FasCD95, TNF-R1, DR4/5 and blocking signaling on the EGFR and K-Ras MAPK/ERK pathway, as well as through the intrinsic pathway activating tBID, which promotes the amplification of the apoptotic signal due to the activation of caspase-3, and consequently caspase-7. In addition to the activation of the IIb complex associated with cell death due to necroptosis.
Efficient and Stable Delivery of Multiple Genes to Fish Cells by a Modified Recombinant Baculovirus System
Authors: Wang Qian , Fang Jian , Pan Qihua , Wang Yizhou , Xue Ting , Li Lingyu , Chen Tiansheng
Published: November 2018

The recombinant baculovirus has been widely used as an efficient tool to mediate gene delivery into mammalian cells but has barely been used in fish cells. In the present study, we constructed a recombinant baculovirus containing the dual-promoter cytomegalovirus (CMV) and white spot syndrome virus (WSSV) immediate-early gene 1 (ie1) (WSSV ie1), followed by a puromycin–green fluorescent protein (Puro-GFP, pf) or puromycin–red fluorescent protein (Puro-RFP, pr) cassette, which simultaneously allowed for easy observation, rapid titer determination, drug selection, and exogenous gene expression. This recombinant baculovirus was successfully transduced into fish cells, including Mylopharyngodon piceus bladder (MPB), fin (MPF), and kidney (MPK); Oryzias latipes spermatogonia (SG3); and Danio rerio embryonic fibroblast (ZF4) cells. Stable transgenic cell lines were generated after drug selection, which was further verified by Western blot. A cell monoclonal formation assay proved the stable heredity of transgenic MPB cells. In addition, a recombinant baculovirus containing a pr cassette and four transcription factors for induced pluripotent stem cells (iPSC) was constructed and transduced into ZF4 cells, and these exogenous genes were simultaneously delivered and transcribed efficiently in drug-selected ZF4 cells, proving the practicability of this modified recombinant baculovirus system. We also proved that the WSSV ie1 promoter had robust activity in fish cells in vitro and in vivo. Taken together, this modified recombinant baculovirus can be a favorable transgenic tool to obtain transient or stable transgenic fish cells.