bluuebunny/crossref_metadata_2025 huggingface dataset:
The safety and efficacy of N-acetylcysteine as an augmentation in the treatment of obsessive-compulsive disorder in adults: a systematic review and meta-analysis of randomized clinical trials
Authors: Eghdami Shayan , Eissazade Negin , Heidari Mokarar Mohsen , Boroon Mahsa , Orsolini Laura , Shalbafan Mohammadreza
Published: September 2024

Background

Obsessive-compulsive disorder (OCD) ranks as the fourth most prevalent psychiatric disorder, with selective serotonin reuptake inhibitors (SSRIs) as its mainstay pharmacological treatment. However, approximately 40 to 60% of patients do not adequately respond to initial treatment, highlighting the need for alternative options. N-acetylcysteine (NAC) is one of the several medications that have been used in augmentation with SSRIs to enhance their efficacy.

Objectives

We aimed to investigate the safety and efficacy of NAC, a glutamate-modulating agent, as an augmentation in the treatment of moderate to severe OCD.

Method

We conducted a thorough search across PubMed, Scopus, Web of science, and ProQuest to identify relevant trials published until December 2023. The primary outcome of interest was the mean difference between the Yale-Brown Obsessive-Compulsive Scale (Y-BOCS) scores before and after administrating augmented NAC among patients with moderate to severe OCD. Furthermore, we compared the occurrence of adverse drug events between the experimental and control groups.

Results

We included six randomized controlled trials with 195 patients. The results of our study indicated a positive outcome for the experimental group in terms of the total Y-BOCS score when using the medication for a period of five to eight weeks (p-Value = 0.05). However, no significant difference was observed for durations shorter than five weeks or longer than 12 weeks. Additionally, no significant difference was found between the two groups in terms of the obsession and compulsion Y-BOCS scores. Furthermore, no significant differences were observed in terms of adverse events.

Conclusion

Augmentation of NAC with SSRIs may benefit patients with moderate to severe OCD. However, it is necessary to conduct additional multi-center trials over extended periods to develop a comprehensive strategy for action.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier CRD42023463683.
Physical demands of collegiate basketball practice: a preliminary report on novel methods and metrics
Authors: Huynh Peter , Guadagnino Samuel , Zendler Jessica , Agresta Cristine
Published: September 2024

Knowing the specific physical demands of basketball players can provide useful information for clinical decision making when rehabilitating athletes following injury. The purpose of this observational study was to describe the physical demands of basketball play at the Division I collegiate level using video-based time-motion analysis and introduce a time-efficient alternative method of quantifying demands. Eleven NCAA Division I basketball players (6M, 5F; 4 guards, 4 centers, 3 forwards) participated in the study. Video footage was collected from four practices (2 men's, 2 women's) and used to quantify the types and frequencies of player movements based on definitions from seminal work. A second and simpler method was also used to classify movement. A two-way ANOVA was used to assess significant differences in movement by team (men's, women's) and position (guard, forward, center). There were significant differences in counts of stand/walk (p < 0.001), jog (p = 0.012), run (p = 0.001), stride/sprint (p = 0.04), and medium-intensity shuffling (p < 0.001) per minute and proportion of practice time spent in bodyweight (p < 0.001) or above-bodyweight (p < 0.001) loading between teams. There were significant differences for jog (p = 0.001) and transition (p = 0.07) rates across positions. Position and team are important considerations for rehabilitation and return-to-sport clearance. Quantification of these demands can be reliably acquired through video analysis using a simplified method (estimated foot load) or using traditional methods of movement classification and counts, particularly when applying descriptors that better capture the current style of play.
Women coaches leadership development programme: an evaluation study of programme effectiveness
Authors: Jowett Sophia , Slade Katelynn , Gosai Jyoti , Davis Louise
Published: September 2024

Introduction

Women's sport has been experiencing continuous growth, yet the low levels of women coaches in the United Kingdom (UK) suggests that the sport is missing out on potential talent. Guided by empirical research, a women-only leadership development programme was designed and implemented by UK Sport to a cohort of 20 coaches from January to June 2021. The main characteristics of the programme included raising awareness of gender bias while at the same time focusing on women coaches' leadership purpose and skills within a safe environment that supports coaches to build their leader identity.

Methods

Kirkpatrick's (1998) four-level model was employed to guide the evaluation of programme effectiveness: reactions, learnings, applications and results. Interviews were conducted with 17 participating coaches.

Results

Content analysis of the qualitative data revealed five general categories (a) evaluations were mainly positive highlighting the aspects of the programme they liked, enjoyed and made most impression onto them; (b) learnings that impacted their work directly and immediately emerged as was the importance of on-going development; and (c) the majority of the women desired progression and transitioning to senior coach leadership positions was felt within their grasp; nonetheless, (d) challenges emerged and were described as organisational (e.g., recruitment, remuneration) and personal (e.g., work-life balance, childcare).

Discussion

Overall, the effectiveness of the programme was captured in its capacity to raise awareness, develop knowledge, build connections, and inspire so much so that its effects translated to many of these women moving to more senior leadership positions post-programme. Practically, this evaluation highlights that investment in individual coaches is part of the systemic change required to bring about gender balance in the coach workforce.
Effects of a 12-week training program in outdoor gym equipment in morphological and functional parameters, quality of life, and physical activity levels in older adults
Authors: Barbosa Welmo A. , Machado Alexandre F. , Bergamin Marco , Gobbo Stefano , Bullo Valentina , Pontes Junior Francisco Luciano , Evangelista Alexandre L. , Scartoni Fabiana R. , Rica Roberta L. , Bocalini Danilo S.
Published: September 2024

Introduction

This study aimed to investigate the effectiveness of a supervised physical training program with controlled cadence on functional fitness parameters, quality of life perception, and physical activity level in older adults.

Methods

Sixty physically independent older adults were randomly divided into three groups: Supervised Training (ST,n = 20), Unsupervised Training (UT,n = 20), and a Control Group (C,n = 20). The ST and UT groups participated in a 12-week program, performing exercises three times a week for 30 min. The ST group had structured weekly sessions consisting of a 5-minute warm-up (walking at 60% of max heart rate), 20 sets of 30 s at a moderate pace controlled by a metronome with 30 s of passive recovery, and a 5-minute cool-down on non-consecutive days. The UT group trained spontaneously using senior gym equipment, including elliptical machines, rowing machines, air skiers, and leg press machines. The control group maintained their usual daily routines throughout the study. Parameters evaluated included body mass, body mass index (BMI), muscle thickness (biceps brachii, triceps brachii, and vastus lateralis), and functional capacity tests (walking 10 m [W10 m], rising from a sitting position [RSP], rising from the prone position [RVDP], sitting and rising from a chair and moving around the house [SRCW]). Quality of life was assessed across physical, psychological, environmental, and social domains, and physical activity levels were also measured.

Results

No significant changes (p > 0.05) in body mass, BMI, or muscle thickness were observed between groups before and after the intervention. However, significant time effects in functional fitness tests were found only in the ST group for W10 m (p = 0.0469), RVDP (p < 0.0004), RSP (p < 0.0001), and SRCW (p < 0.0001). Quality of life improved significantly over time in the ST and UT groups across all domains (p < 0.0001). Both ST and UT groups also showed significant increases in weekly physical activity time (p > 0.0001).

Conclusion

12 weeks of training improved quality of life perception and physical activity levels in older adults.
Case report: Intracranial epidermoid cyst in a cat
Authors: Terao Masashi , Uemura Takashi , Hasegawa Hiroki , Ashida China , Ehara Ikuya , Ozawa Tsuyoshi , Kamishina Hiroaki
Published: September 2024

A 9-year-old American Shorthair, castrated male, weighing 4.3 kg was presented to our hospital because of intermittent ataxia and tetraparesis for 6 weeks. On presentation, the cat was in a stupor and on recumbency, and had vertical nystagmus in both eyes. These clinical signs suggested a brainstem disorder. MRI showed a mass lesion in the caudal aspect of the fourth ventricle with hyperintensity on T2-weighted and FLAIR imaging, low-intensity on T1-weighted imaging, and enhanced margins on post-contrast T1-weighted imaging. The mass compressed the fourth ventricle, causing obstructive hydrocephalus. A second cystic lesion was found rostral to the cerebellum. After MRI, the cat experienced respiratory difficulties and the mass was removed by emergency craniectomy. Although the mass including the cyst wall was successfully removed, the cat was euthanized because spontaneous breathing did not return. The mass was histopathologically diagnosed as epidermoid cyst. A biopsy to the rostral cystic lesion had not been performed and therefore the etiology of this lesion remained unclear. This is the first case of feline intracranial epidermoid cyst in which MRI and surgical excision were performed. MRI findings were similar to those in humans and dogs, suggesting that imaging studies are useful in cats for the diagnosis of intercranial epidermoid cyst.
Legaltech el futuro del mundo jurídico
Authors:   Universidad Internacional del Ecuador, Obando Balseca Gabriela Estefanía , Baeza Regalado Jorge Hernán , Fernández Schatzer Marcelo Nicolás
Published: September 2024

LEGALTECH EL FUTURO DEL MUNDO JURÍDICO proporciona un análisis exhaustivo de la interacción entre la tecnología, especialmente la inteligencia artificial (IA), y el derecho administrativo en el contexto ecuatoriano. La obra está estructurada en tres partes principales, cada una abordando dimensiones críticas de esta intersección. En la primera parte, el manual explora detalladamente el marco jurídico-administrativo de Ecuador, delineando los principios fundamentales que rigen la función ejecutiva y el procedimiento administrativo. Esta sección incluye una discusión sobre la eficacia, eficiencia, transparencia y juridicidad de los actos administrativos, además de tratar sobre el procedimiento sancionador administrativo y su importancia en la regulación de las conductas dentro del marco de la función pública. La segunda parte del libro se centra en la tecnología Legaltech, destacando el papel transformador de la IA en el ámbito legal. Se discuten los desafíos éticos y normativos que emergen de la aplicación de IA en prácticas legales, además de analizar cómo las tecnologías disruptivas pueden ser empleadas para optimizar procedimientos legales y administrativos. Además, se examinan las oportunidades que la IA presenta para la innovación en la prestación de servicios legales y en la gestión pública. Finalmente, la tercera parte integra las dos primeras, explorando cómo la IA puede aplicarse a los procedimientos administrativos para mejorar la eficiencia, efectividad y transparencia de la administración pública. Se propone un marco regulatorio específico para la implementación ética y efectiva de la IA en la administración pública, junto con estudios de caso que ilustran su aplicación práctica. En conjunto, el libro ofrece un recurso valioso para académicos, profesionales y funcionarios interesados en la convergencia de la tecnología y el derecho, proporcionando una base sólida para la comprensión y aplicación de Legaltech en el contexto jurídico y administrativo de Ecuador.
Strigolactone Alleviates NaCl Stress by Regulating Antioxidant Capacity and Hormone Levels in Rice (Oryza sativa L.) Seedlings
Authors: Zhang Jianqin , Feng Naijie , Zheng Dianfeng , Khan Aaqil , Du Youwei , Wang Yaxing , Deng Rui , Wu Jiashuang , Xiong Jian , Sun Zhiyuan , Zhang Qicheng , Wang Mingxin
Published: September 2024

Salt stress is a key environmental factor altering rice plant growth. Strigolactones (GR24) play a vital role in responding to various abiotic stresses and regulating plant growth. However, the regulatory mechanisms of SLs on rice seedlings under salt stress have not yet been clarified. A pot experiment was undertaken to evaluate the effects of GR24 soaking on the rice variety ‘Huanghuazhan’ (salt-sensitive) seedling growth, antioxidant metabolism, and endogenous hormones under NaCl stress. Results showed that NaCl stress significantly inhibited rice growth; disrupted antioxidant enzymes activity; and increased the content of soluble proteins (SPs), proline (Pro), malondialdehyde (MDA) and hydrogen–peroxide (H2O2). GR24 significantly improved photosynthetic pigments and antioxidant–enzyme activities, including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate–peroxidase (APX); increased SP, ascorbic acid (AsA); and reduced glutathione (GSH) content and MDA, H2O2, and Pro content, resulting in the mitigation of oxidative injury caused by NaCl stress. Moreover, GR24 significantly increased the content of strigolactones (SLs), cytokinin (CTK), auxin (IAA), Gibberellin A3 (GA3), and IAA/ABA and CTK/ABA ratios and decreased the abscisic acid (ABA). Findings indicated that GR24 alleviated oxidative damage caused by NaCl stress by increasing photosynthetic and antioxidant capacity and maintaining the balance of endogenous hormones, thus improving the salt tolerance of rice seedlings.
Effect of Organic Fertilizer Application on Microbial Community Regulation and Pollutant Accumulation in Typical Red Soil in South China
Authors: Sun Qinghong , Zhang Qiao , Huang Zhijie , Wei Chang , Li Yongtao , Xu Huijuan
Published: September 2024

Returning livestock manure to the cropland as organic fertilizer is a sustainable and environmentally friendly treatment method, but its application also alters the soil microenvironment. However, the impact of soil microbial community disturbance and pollutant accumulation from different types of organic fertilizers remains largely unknown in South China. To fill this gap, we investigated the effects of organic fertilizers, including chicken manure, pig manure and vermicompost on the soil bacterial and fungal communities and environmental risks. The results show that applying organic fertilizer effectively increases the soil nutrient content. High-throughput sequencing of bacteria and fungi showed that the application of different organic fertilizers had differential effects on microbial community structure, with the highest number of microbe-specific OTUs in the vermicomposting treatment. Additionally, this study found no risk of heavy metal (Cu, Zn, Pb, Cr and Cd) contamination from short-term organic fertilizer application, but there was a risk of antibiotic (ENR and CHL) contamination. Functional microorganisms regulating heavy metals and antibiotics were identified by RDA analysis. This study facilitates the screening of types of organic fertilizers that can be safely returned to the field as well as developing strategies to regulate functional microbes.
Are Water Use Efficiency and Effectiveness Relatively Lower in Arid Zones? Comparative Analyses of the Water Productivity of Typical Crops
Authors: Zhang Yanfei , Long Aihua , Zhang Pei , Deng Xiaoya , Gu Xinchen
Published: September 2024

Agriculture is the largest water user of all sectors. In arid regions in particular, achieving efficient water use in agriculture is an important way to solve water scarcity. However, the difference in water use efficiency between arid and humid regions has long been a focus of academic debate. Many studies consider water use efficiency to be higher in humid areas due to the abundance of water resources. This view is based on the fact that less irrigation in humid areas may lead to higher crop yields and better conditions for agricultural production; however, it ignores the efforts of researchers and agricultural workers in arid zones who have attempted to develop efficient water-saving technologies, as well as the effect of natural conditions on agricultural production. Correctly evaluating the efficiency of agricultural water use in arid zones is important for achieving efficient use of water resources, as well as for water management decisions. This study calculates the yield structure and water productivity of typical crops in both arid and humid regions in China based on the footprint theory and other methodologies. This approach allows for an accurate assessment of irrigation water benefits in various regions, providing a scientific basis for improving agricultural water use efficiency under different climatic conditions. The study results indicate that the average reliance on blue water for wheat and cotton gradually increases from 49.9% to 93.6% as regional aridity intensifies, ranging from the Central China Humid Region to the Northwest China Arid Region. Similarly, the average contribution of blue water to crop yield rises from 31.0% to 100%, while irrigation water productivity increases from 0.27 kg·m−3 to 0.53 kg·m−3. Finally, this study concludes that, in arid zones with lower precipitation and more hours of sunshine, a higher dependence on blue water for crop growth and development leads to a higher productivity of irrigation water. In addition, in arid zones, the focus should be on optimizing the use of irrigation water and improving irrigation technology and efficiency, while, in humid zones, there should be more use of natural precipitation to efficiently reduce dependence on irrigation water.
Recent Advances in Biomimetic Methods for Tillage Resistance Reduction in Agricultural Soil-Engaging Tools
Authors: Wang Xuezhen , Zhang Shihao , Du Ruizhi , Zhou Hanmi , Ji Jiangtao
Published: September 2024

The high tillage resistance of agricultural soil-engaging tools (TASTs) in farmland operations (e.g., tillage, sowing, crop management, and harvesting) increases fuel consumption and harmful gas emissions, which negatively affect the development of sustainable agriculture. Biomimetic methods are promising and effective technologies for reducing the TASTs and have been developed in the past few years. This review comprehensively summarizes the typical agricultural soil-engaging tools (ASETs) and their characteristics and presents existing biomimetic methods for decreasing TASTs. The introduction of TAST reduction was performed on aspects of tillage, sowing, crop management, and harvesting. The internal mechanisms and possible limitations of current biomimetic methods for various ASETs were investigated. The tillage resistance reduction rates of ASETs, as affected by various biomimetic methods, were quantitatively compared under different soil conditions with statistical analyses. Additionally, three future research directions were recommended in the review to further reduce TASTs and encourage the development of sustainable agriculture.
Study on the Natural Ventilation Model of a Single-Span Plastic Greenhouse in a High-Altitude Area
Authors: Li Youyu , Zhao Shumei , Dai Anguo , Zhang Jingfu , Fan Zilong , Ding Tao
Published: September 2024

The natural ventilation model plays a crucial role in greenhouse environmental control. It has been extensively studied by previous researchers, but it is limited to low-altitude areas. This study established a numerical model of single-span plastic greenhouses in high-altitude areas. The model was validated using measured data, showing a good agreement between the measured and simulated values. By setting boundary conditions based on on-site monitoring data, ventilation rates were extracted under different conditions for numerical simulations. Through nonlinear fitting, an empirical formula for natural ventilation rates, with a determination coefficient (R2) of 0.9724, was derived. The formula was validated through an energy balance analysis of indoor air. Different ventilation opening sizes were simulated to derive an empirical formula for natural ventilation rates based on opening size. Building on this, the relationship between plant height and ventilation rate was analyzed. As the dominant factors of natural ventilation change with environmental fluctuations, this study also proposed the threshold wind speed for wind pressure ventilation, thermal pressure ventilation, and coupled ventilation, filling the knowledge gap in relevant ventilation rate calculations. This is the first time that a natural ventilation model of single-span plastic greenhouses in high-altitude areas has been proposed, providing the basis in terms of modeling for the further development of local facility agriculture.
Genome-Wide Association Study Reveals Marker–Trait Associations for Heat-Stress Tolerance in Sweet Corn
Authors: Yang Quannv , Guo Zifeng , Zhang Jianan , Wang Yunbo , Xu Yunbi , Nian Hai
Published: September 2024

Sweet corn (Zea mays var. rugosa Bonaf.) is a crop with a high economic benefit in tropical and subtropical regions. Heat tolerance analysis and heat-tolerant gene mining are of great significance for breeding heat-resistant varieties. By combining improved genotyping using targeted sequencing (GBTS) with liquid chip (LC) technology, a high-density marker array containing 40 K multiple single polynucleotide polymorphisms (mSNPs) was used to genotype 376 sweet corn inbred lines and their heat-stress tolerance was evaluated in the spring and summer of 2019. In general, plant height, ear height and the number of lateral branches at the first level of the male flowers were reduced by 24.0%, 36.3%, and 19.8%, respectively. High temperatures in the summer accelerated the growth process of the sweet corn, shortening the days to shedding pollen by an average of 21.6% compared to the spring. A genome-wide association study (GWAS) identified 85 significant SNPs distributed on 10 chromosomes. Phenotypes in the spring and summer were associated with the 21 and 15 loci, respectively, and significant phenotypic differences between the two seasons caused by the temperature change were associated with the 49 SNP loci. The seed setting rate (SSR) was more susceptible to heat stress. An annotation analysis identified six candidate genes, which are either heat shock transcription factors (Hsfs) or heat shock proteins (Hsps) in Arabidopsis and rice (Oryza sativa), and these candidate genes were directly and indirectly involved in the heat-resistant response in the sweet corn. The current findings provide genetic resources for improving the heat-stress tolerance of sweet corn by molecular breeding.
Traditional Strategies and Cutting-Edge Technologies Used for Plant Disease Management: A Comprehensive Overview
Authors: Akhtar Hira , Usman Muhammad , Binyamin Rana , Hameed Akhtar , Arshad Sarmad Frogh , Aslam Hafiz Muhammad Usman , Khan Imran Ahmad , Abbas Manzar , Zaki Haitham E. M. , Ondrasek Gabrijel , Shahid Muhammad Shafiq
Published: September 2024

Agriculture plays a fundamental role in ensuring global food security, yet plant diseases remain a significant threat to crop production. Traditional methods to manage plant diseases have been extensively used, but they face significant drawbacks, such as environmental pollution, health risks and pathogen resistance. Similarly, biopesticides are eco-friendly, but are limited by their specificity and stability issues. This has led to the exploration of novel biotechnological approaches, such as the development of synthetic proteins, which aim to mitigate these drawbacks by offering more targeted and sustainable solutions. Similarly, recent advances in genome editing techniques—such as meganucleases (MegNs), zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)—are precise approaches in disease management, but are limited by technical challenges and regulatory concerns. In this realm, nanotechnology has emerged as a promising frontier that offers novel solutions for plant disease management. This review examines the role of nanoparticles (NPs), including organic NPs, inorganic NPs, polymeric NPs and carbon NPs, in enhancing disease resistance and improving pesticide delivery, and gives an overview of the current state of nanotechnology in managing plant diseases, including its advantages, practical applications and obstacles that must be overcome to fully harness its potential. By understanding these aspects, we can better appreciate the transformative impact of nanotechnology on modern agriculture and can develop sustainable and effective strategies to mitigate plant diseases, ensuring enhanced agricultural productivity.
Effects of Different Processed Diets on Growth Performance, Blood Parameters, Hair Quality and Fecal Microbiota in Ragdoll Cats
Authors: Wang Peng , Tian Xin , Feng Jie
Published: September 2024

In recent years, there has been ongoing debate about the dietary choices for pet cats, particularly regarding three options: extruded dry food, cooked meat, and raw meat. Determining which diet is most suitable for a cat’s healthy growth still requires substantial empirical support. Our study aimed to evaluate the effects of feeding Ragdoll cats (n = 5/group) extruded dry food (ED), cooked meat (CM), and raw meat (RM) on their growth performance, apparent digestibility, fur condition, blood parameters, fecal scores, and gut microbiota composition. However, our results indicate that different types of diets did not significantly affect the daily weight gain of Ragdoll cats. The CM group showed a significant improvement in the digestibility of dry matter, fat and protein compared to the ED group (p < 0.05) but no improvement in that of fat compared to the RM group. Compared to the ED group, both the CM and RM groups showed significant improvements in fur condition while exhibiting a significant decrease in fecal scores (p < 0.05). The CM and RM groups exhibited enhanced serum antioxidant capacity (p < 0.05) and increased immunity in the cats (p < 0.05). Immunity enhancement in the CM group was significantly higher than that in the RM group(p < 0.05). The ED group showed an increase in the abundance of beneficial bacteria in Ragdoll cat intestines, while the CM and RM groups showed enhancements in the innate microbiota of feline animals. These data, to some extent, suggest that CM is the most suitable diet for Ragdoll cats, but further research on intestine microbiota is still needed. These study findings provide a reference for purebred pet breeding purposes.
Effects of Feeding Systems on the Growth Performance, Carcass Characteristics, and Meat Quality in Sheep: A Meta-Analysis
Authors: Wang Wenjie , Zhang Xiaoan , Wei Huiqing , Wang Sunze , Ye Yang , He Li , Zhang Kefan , Lu Yuan , Zhang Zijun , Huang Yafeng
Published: September 2024

Meat quality is important in the meat-production chain. Conflicting reports of the effects of feeding systems on sheep growth performance and meat quality exist. By way of meta-analysis, we reviewed the literature on the growth and slaughter performance, and meat quality of lambs that grazed solely on pasture, those that grazed on pasture but received a dietary supplement, and those were exclusively fed indoors. The relevant literature comprised 28 papers, from which response variables of interest were obtained. Compared with stall-fed sheep, pasture-grazing led to significantly (p < 0.05) lower average daily gain, slaughter live weight, hot carcass weight, cold carcass weight, and similar dressing percentage, but pasture-grazed sheep fed a supplement had similar (p > 0.05) values for each of these attributes to stall-fed sheep. The quality of the longissimus muscle from lambs that grazed either exclusively on pasture or pasture with a supplement had significantly (p < 0.05) lower lightness and intramuscular fat content, and significantly (p < 0.05) higher yellowness, Warner–Bratzler shear force, and protein content than meat from stall-fed sheep. We conclude that sheep that have fed exclusively on pasture have lower carcass yield and meat edibility, but improved meat quality, and that pasture-fed sheep that received a supplement had comparable carcass attributes, but greater meat color and health quality than stall-fed sheep.
Non-Targeted Metabolomics of Serum Reveals Biomarkers Associated with Body Weight in Wumeng Black-Bone Chickens
Authors: Wang Zhong , Yu Xuan , Yang Shenghong , Zhao Mingming , Wang Liqi
Published: September 2024

Growth performance is an important economic trait of broilers but the related serum metabolomics remains unclear. In this study, we utilized non-targeted metabolomics using ultra-high-performance liquid phase tandem mass spectrometry (UHPLC-MS/MS) to establish metabolite profiling in the serum of Chinese Wumeng black-bone chickens. The biomarker metabolites in serum associated with growth performance of chickens were identified by comparing the serum metabolome differences between chickens that significantly differed in their weights at 160 days of age when fed identical diets. A total of 766 metabolites were identified including 13 differential metabolite classes such as lipids and lipid-like molecules, organic acids and their derivatives, and organoheterocyclic compounds. The results of difference analysis using a partial least squares discriminant analysis (PLS-DA) model indicated that the low-body-weight group could be differentiated based on inflammatory markers including prostaglandin a2, kynurenic acid and fatty acid esters of hydroxy fatty acids (FAHFA), and inflammation-related metabolic pathways including tryptophan and arachidonic acid metabolism. In contrast, the sera of high-body-weight chickens were enriched for riboflavin and 2-isopropylmalic acid and for metabolic pathways including riboflavin metabolism, acetyl group transfer into mitochondria, and the tricarboxylic acid (TCA) cycle. These results provide new insights into the practical application of improving the growth performance of local chickens.
Combining Transcriptomics and Proteomics to Screen Candidate Genes Related to Bovine Birth Weight
Authors: Wang Xiuyuan , Liu Ruili , Chen Zhenpeng , Zhang Renzheng , Mei Yanfang , Miao Xiuping , Bai Xuejin , Dong Yajuan
Published: September 2024

The placenta is a vital organ in bovine reproduction, crucial for blood supply, nutrient transport, and embryonic development. It plays an essential role in the intrauterine growth of calves. However, the molecular mechanisms governing placental function in calves remain inadequately understood. Methods: We established transcriptome and proteome databases for low-birth-weight (LB) and high-birth-weight (HB) calf placentae, identifying key genes and proteins associated with birth weight through bioinformatics analyses that included functional enrichment and protein–protein interactions (PPIs). Both mRNA and protein levels were validated. Results: A total of 1494 differentially expressed genes (DEGs) and 294 differentially expressed proteins (DEPs) were identified when comparing the LB group to the HB group. Furthermore, we identified 53 genes and proteins exhibiting significant co-expression across both transcriptomic and proteomic datasets; among these, 40 were co-upregulated, 8 co-downregulated, while 5 displayed upregulation at the protein level despite downregulation at the mRNA level. Functional enrichment analyses (GO and KEGG) and protein–protein interaction (PPI) analysis indicate that, at the transcriptional level, the primary factor contributing to differences in calf birth weight is that the placenta of the high-birth-weight (HB) group provides more nutrients to the fetus, characterized by enhanced nutrient transport (SLC2A1 and SLC2A11), energy metabolism (ACSL1, MICALL2, PAG2, COL14A1, and ELOVL5), and lipid synthesis (ELOVL5 and ELOVL7). In contrast, the placenta of the low-birth-weight (LB) group prioritizes cell proliferation (PAK1 and ITGA3) and angiogenesis. At the protein level, while the placentae from the HB group exhibit efficient energy production and lipid synthesis, they also demonstrate reduced immunity to various diseases such as systemic lupus erythematosus and bacterial dysentery. Conversely, the LB group placentae excel in regulating critical biological processes, including cell migration, proliferation, differentiation, apoptosis, and signal transduction; they also display higher disease immunity markers (COL6A1, TNC CD36, CD81, Igh-1a, and IGHG) compared to those of the HB group placentae. Co-expression analysis further suggests that increases in calf birth weight can be attributed to both high-efficiency energy production and lipid synthesis within the HB group placentae (ELOVL5, ELOVL7, and ACSL1), alongside cholesterol biosynthesis and metabolic pathways involving CYP11A1 and CYP17A1. Conclusion: We propose that ELOVL5, ELOVL7, ACSL1, CYP11A1, and CYP17A1 serve as potential protein biomarkers for regulating calf birth weight through the modulation of the fatty acid metabolism, lipid synthesis, and cholesterol levels.
Recent Advances in the Extraction and Characterization of Bioactive Compounds from Corn By-Products
Authors: Ramírez-Esparza Ulises , Agustín-Chávez María Cristina , Ochoa-Reyes Emilio , Alvarado-González Sandra M. , López-Martínez Leticia X. , Ascacio-Valdés Juan A. , Martínez-Ávila Guillermo C. G. , Prado-Barragán Lilia Arely , Buenrostro-Figueroa José Juan
Published: September 2024

Maize comes in a variety of colors, including white, yellow, red, blue, and purple, which is due to the presence of phytochemicals such as carotenoids, anthocyanins, flavonoids, phytosterols, and some hydroxycinnamic acid derivatives. In Mexico, maize is primarily grown for human consumption; however, maize residues comprise 51–58% of the total maize plant weight (stalks, leaves, ears, and husks) and are mainly used as livestock feed. These residues contain numerous bioactive compounds that interest the industry for their potential health benefits in preventing or treating degenerative diseases. This review explores the current knowledge and highlights key aspects related to the extraction methods and different techniques for identifying the bioactive compounds found in maize by-products.
Anti-Inflammatory and Anticancer Effects of Anthocyanins in In Vitro and In Vivo Studies
Authors: Kowalczyk Tomasz , Muskała Martyna , Merecz-Sadowska Anna , Sikora Joanna , Picot Laurent , Sitarek Przemysław
Published: September 2024

Anthocyanins, a class of flavonoid compounds responsible for the vibrant colors of many fruits and vegetables, have received considerable attention in recent years due to their potential health benefits. This review, focusing on evidence from both in vitro and in vivo studies, provides a comprehensive overview of the current state of knowledge regarding the health-promoting properties of anthocyanins. The chemical structure and diversity of anthocyanins, their bioavailability, and their mechanisms of action at the cellular and molecular level are examined. Research on the antioxidant, anti-inflammatory, anticancer, and neuroprotective effects of anthocyanins is critically reviewed. Special emphasis is placed on the role of anthocyanins in the prevention and treatment of chronic diseases such as cardiovascular diseases, diabetes, and neurodegenerative diseases. This review also discusses the challenges of translating in vitro findings to in vivo and highlights the importance of considering dose, bioavailability, and metabolism when assessing the therapeutic potential of anthocyanins. This review concludes with the identification of gaps in current research and suggestions for future directions for anthocyanin studies, including the need for more long-term clinical trials and investigations into potential synergistic effects with other phytochemicals. This comprehensive analysis highlights the promising role of anthocyanins in promoting human health and provides valuable insights for researchers, health professionals, and the nutraceutical industry. This study provides new insights, as it comprehensively investigates the dual anti-inflammatory and anticancer effects of anthocyanins in both in vitro and in vivo models. By uncovering the biological properties of anthocyanins from a variety of natural sources, this research not only expands our knowledge of the action of these compounds at the cellular level, but also enhances their clinical relevance through in vivo validation. Furthermore, the innovative use of anthocyanins may lead to important advances in their therapeutic application in the future.
Oxidative Stability of Fish Oil-Loaded Nanocapsules Produced by Electrospraying Using Kafirin or Zein Proteins as Wall Materials
Authors: Rahmani-Manglano Nor E. , Fallahasghari Elnaz Z. , Mendes Ana C. , Andersen Mogens L. , Guadix Emilia M. , Chronakis Ioannis S. , García-Moreno Pedro J.
Published: September 2024

The encapsulation of fish oil by monoaxial electrospraying using kafirin or zein proteins as hydrophobic wall materials was investigated. Kafirin resulted in spherical fish oil-loaded nanocapsules (>50% of capsules below 1 µm), whereas zein led to fish oil-loaded nanocapsules with non-spherical morphology (>80% of capsules below 1 µm). Both hydrophobic encapsulating materials interacted with fish oil, successfully entrapping the oil within the protein matrix as indicated by Fourier-transform infrared spectroscopy (FTIR) and Raman spectroscopy results. FTIR also suggested hydrogen bonding between fish oil and the proteins. Trapped radicals in the encapsulation matrix that were detected by electron paramagnetic resonance (EPR), indicated oxidation during electrospraying and storage. Results from isothermal (140 °C) differential scanning calorimetry (DSC) denoted that the encapsulation of fish oil by electrospraying using both kafirin or zein as wall materials protected fish oil from oxidation. In particular, the zein-based nanocapsules were 3.3 times more oxidatively stable than the kafirin-based nanocapsules, which correlates with the higher oil encapsulation efficiency found for zein-based capsules. Thus, this study shows that kafirin might be considered a hydrophobic wall material for the encapsulation of fish oil by electrospraying, although it prevented lipid oxidation to a lower extent when compared to zein.
Phytotoxicity Testing of Atmospheric Polycyclic Aromatic Hydrocarbons
Authors: Tumurbaatar Selenge , Kováts Nora , Hubai Katalin
Published: September 2024

Atmospheric polycyclic aromatic hydrocarbons (PAHs) have well-known phytotoxicity on higher plants. However, while numerous bioindication studies have been targeted on how different symptoms indicate the deleterious effects of PAHs in the field, laboratory-scale phytotoxicity tests are much rarer. While ecotoxicity tests might rely on the very same end-points as bioindication studies, they have to comply with quality assurance criteria, repeatability being the most important. As such, proper reporting involves the description of the test compound, experimental design and conditions, test organism used, and end-points measured. The recent review intends to give an overview of studies available in the literature complying with these requirements. PAHs occur in the atmosphere both in gaseous form and bound to particles. As plants are exposed to both phases, test protocols available represent different exposure pathways, fumigation chambers vs. direct foliar treatment. Reported studies, therefore, are grouped based on the exposure route they intend to simulate.
Editorial to the Special Issue “Molecular and Cellular Mechanisms of CVD: Focus on Atherosclerosis”
Authors: Nikiforov Nikita G.
Published: September 2024

The current Special Issue, “Molecular and Cellular Mechanisms of CVD: Focus on Atherosclerosis”, is dedicated to exploring the various mechanisms involved in atherogenesis [...]
Assessing Cardiovascular Risk with Coronary Artery Calcium and Carotid Intima-Media Thickness in Patients with Negative Stress Echocardiography
Authors: Kim Narae , Hwang Hui-Jeong , Yang In-Ho
Published: September 2024

Background: The role of treadmill stress echocardiography (TSE) in symptomatic patients may be limited. We evaluated whether carotid intima-media thickness (cIMT) and coronary artery calcium (CAC) scores can predict cardiovascular (CV) outcomes in patients with negative TSE. Methods: Patients who had negative TSE and measured cIMT or CAC scoring were enrolled and followed up. The primary CV outcome was defined as a composite of acute coronary syndrome, coronary revascularization, heart failure, stroke, and CV death. Results: Overall, 1095 patients participated. The median follow-up duration was 5.8 years. Patients with increased cIMT and CAC scores experienced a high incidence of primary CV outcomes (normal vs. increased group on cIMT and CAC scoring: 4.4% vs. 20.0% and 0.4% vs. 25.0%, respectively, p < 0.001). In the Cox proportional hazard model, increased cIMT and CAC scores were associated with increased primary CV outcomes (adjusted hazard ratio [95% confidence interval], p-value for increased cIMT and increased CAC scores = 2.939 [1.241–6.960], p = 0.014 and 45.192 [5.497–371.505], p < 0.001, respectively). Conclusions: Patients with increased cIMT and CAC scores have poor CV outcomes even though they have negative TSE results, and therefore, they should be carefully monitored.
Online Inspection Method and System Design for Screw Threads of Rebar Head Based on Machine Vision
Authors: Liu Li , Liu Zijin , Qian Xuefei
Published: September 2024

An online inspection method based on machine vision was proposed and validated to address the issues of high work intensity, low efficiency, low accuracy, and risk of missed inspection in traditional sampling methods for screw threads of rebar head. Firstly, an industrial camera was used to capture real-time images of the processed rebar thread heads, preprocess the images, and locate the target positions in the images to reduce the complexity and running time of subsequent algorithms. Then, the Canny operator was used to roughly extract the edge feature information of the rebar head, and the Shi–Tomasi algorithm was used for corner inspection to achieve precise optimization of sub-pixel level corners. Based on robust linear regression, the diagonal points were fitted with lines to detect the corresponding size parameters. Finally, an inspection system on screw threads of rebar head parameter was designed and developed, which consisted of an image-acquisition device, Siemens PLC controller, and inspection software. Test results show that this method can achieve online inspection without contact, with inspection accuracy reaching the micrometer level, and 8–10 rebar heads can be inspected per second.
Physically Guided Estimation of Vehicle Loading-Induced Low-Frequency Bridge Responses with BP-ANN
Authors: Lu Xuzhao , Qu Guang , Sun Limin , Xia Ye , Sun Haibin , Zhang Wei
Published: September 2024

The intersectional relationship in bridge health monitoring refers to the mapping function that correlates bridge responses across different locations. This relationship is pivotal for estimating structural responses, which are then instrumental in assessing a bridge’s service status and identifying potential damage. The current research landscape is heavily focused on high-frequency responses, especially those associated with single-mode vibration. When it comes to low-frequency responses triggered by multi-mode vehicle loading, a prevalent strategy is to regard these low-frequency responses as “quasi-static” and subsequently apply time-series prediction techniques to simulate the intersectional relationship. However, these methods are contingent upon data regarding external loading, such as traffic conditions and air temperatures. This necessitates the collection of long-term monitoring data to account for fluctuations in traffic and temperature, a task that can be quite daunting in real-world engineering contexts. To address this challenge, our study shifts the analytical perspective from a static analysis to a dynamic analysis. By delving into the physical features of bridge responses of the vehicle–bridge interaction (VBI) system, we identify that the intersectional relationship should be inherently time-independent. The perceived time lag in quasi-static responses is, in essence, a result of low-frequency vibrations that are aligned with driving force modes. We specifically derive the intersectional relationship for low-frequency bridge responses within the VBI system and determine it to be a time-invariant transfer matrix associated with multiple mode shapes. Drawing on these physical insights, we adopt a time-independent machine learning method, the backpropagation–artificial neural network (BP-ANN), to simulate the intersectional relationship. To train the network, monitoring data from various cross-sections were input, with the responses at a particular section designated as the output. The trained network is now capable of estimating responses even in scenarios where time-related traffic conditions and temperatures deviate from those present in the training data set. To substantiate the time-independent nature of the derived intersectional relationship, finite element models were developed. The proposed method was further validated through the in-field monitoring of a continuous highway bridge. We anticipate that this method will be highly effective in estimating low-frequency responses under a variety of unknown traffic and air temperature conditions, offering significant convenience for practical engineering applications.
A Multi-Objective Prediction XGBoost Model for Predicting Ground Settlement, Station Settlement, and Pit Deformation Induced by Ultra-Deep Foundation Construction
Authors: Huang Guangkai , Liu Zhijian , Wang Yajian , Yang Yuyou
Published: September 2024

Building a deep foundation pit in urban centers frequently confronts issues such as closeness to structures, high excavation depths, and extended exposure durations, making monitoring and prediction of the settlement and deformation of neighboring buildings critical. Machine learning and deep learning models are more popular than physical models because they can handle dynamic process data. However, these models frequently fail to establish an appropriate balance between accuracy and generalization capacity when dealing with multi-objective prediction. This work proposes a multi-objective prediction model based on the XGBoost algorithm and introduces the Random Forest Bayesian Optimization method for hyperparameter self-optimization and self-adaptation in the prediction process. This model was trained with monitoring data from a deep foundation pit at Luomashi Station of Chengdu Metro Line 18, which are characterized by a sand and pebble stratum, cut-and-cover construction, and a depth of 45.5 m. Input data of the model included excavation rate, excavation depth, construction time, shutdown time, and dewatering; output data included settlement, ground settlement, and pit deformation at an operating metro station only 5.7 m adjacent to the ongoing pits. The training effectiveness of the model was validated through its high R2 scores in both training and test sets, and its generalization ability and transferability were evaluated through the R2 calculated by deploying it on adjacent monitoring data (new data). The multi-objective prediction model proposed in this paper will be promising for monitoring the data processing and prediction of settlement of surrounding buildings for ultra-deep foundation pit engineering.