bluuebunny/crossref_metadata_2025 huggingface dataset:
Decision Models and Technology Can Help Psychiatry Develop Biomarkers
Authors: Barron Daniel S. , Baker Justin T. , Budde Kristin S. , Bzdok Danilo , Eickhoff Simon B. , Friston Karl J. , Fox Peter T. , Geha Paul , Heisig Stephen , Holmes Avram , Onnela Jukka-Pekka , Powers Albert , Silbersweig David , Krystal John H.
Published: September 2021

Why is psychiatry unable to define clinically useful biomarkers? We explore this question from the vantage of data and decision science and consider biomarkers as a form of phenotypic data that resolves a well-defined clinical decision. We introduce a framework that systematizes different forms of phenotypic data and further introduce the concept of decision model to describe the strategies a clinician uses to seek out, combine, and act on clinical data. Though many medical specialties rely on quantitative clinical data and operationalized decision models, we observe that, in psychiatry, clinical data are gathered and used in idiosyncratic decision models that exist solely in the clinician's mind and therefore are outside empirical evaluation. This, we argue, is a fundamental reason why psychiatry is unable to define clinically useful biomarkers: because psychiatry does not currently quantify clinical data, decision models cannot be operationalized and, in the absence of an operationalized decision model, it is impossible to define how a biomarker might be of use. Here, psychiatry might benefit from digital technologies that have recently emerged specifically to quantify clinically relevant facets of human behavior. We propose that digital tools might help psychiatry in two ways: first, by quantifying data already present in the standard clinical interaction and by allowing decision models to be operationalized and evaluated; second, by testing whether new forms of data might have value within an operationalized decision model. We reference successes from other medical specialties to illustrate how quantitative data and operationalized decision models improve patient care.
Ten-Year Poverty Alleviation Effect of the Medical Insurance System on Families With Members Who Have a Non-communicable Disease: Evidence From Heilongjiang Province in China
Authors: Xia Qi , Wu Lichun , Tian Wanxin , Miao Wenqing , Zhang Xiyu , Xu Jing , Li Yuze , Shi Baoguo , Wang Nianshi , Yang Huiying , Huang Zhipeng , Yang Huiqi , Li Ye , Shan Linghan , Wu Qunhong
Published: September 2021

Aims: Non-communicable diseases (NCD) drag the NCD patients' families to the abyss of poverty. Medical insurance due to weak control over medical expenses and low benefits levels, may have actually contributed to a higher burden of out-of-pocket payments. By making a multi-dimensional calculation on catastrophic health expenditure (CHE) in Heilongjiang Province over 10 years, it is significant to find the weak links in the implementation of medical insurance to achieve poverty alleviation.

Methods: A logistic regression was undertaken to predict the determinants of catastrophic health expenditure.

Results: The average CHE of households dropped from 18.9% in 2003 to 14.9% in 2013. 33.2% of the households with three or more NCD members suffered CHE in 2013, which was 7.2 times higher than the households without it (4.6%). The uninsured households with cardiovascular disease had CHE of 12.0%, which were nearly 10% points lower than insured households (20.4–22.4%). For Medical Insurance for Urban Employees Scheme enrolled households, the increasing number of NCD members raised the risk of impoverishment from 3.4 to 20.0% in 2003, and from 0.3 to 3.1% in 2008. Households with hospital in-patient members were at higher risk of CHE (OR: 3.10–3.56).

Conclusions: Healthcare needs and utilization are one of the most significant determinants of CHE. Households with NCD and in-patient members are most vulnerable groups of falling into a poverty trap. The targeting of the NCD groups, the poorest groups, uninsured groups need to be primary considerations in prioritizing services that are contained in medical insurance and poverty alleviation.
Enabling Training of Neural Networks on Noisy Hardware
Authors: Gokmen Tayfun
Published: September 2021

Deep neural networks (DNNs) are typically trained using the conventional stochastic gradient descent (SGD) algorithm. However, SGD performs poorly when applied to train networks on non-ideal analog hardware composed of resistive device arrays with non-symmetric conductance modulation characteristics. Recently we proposed a new algorithm, the Tiki-Taka algorithm, that overcomes this stringent symmetry requirement. Here we build on top of Tiki-Taka and describe a more robust algorithm that further relaxes other stringent hardware requirements. This more robust second version of the Tiki-Taka algorithm (referred to as TTv2) 1. decreases the number of device conductance states requirement from 1000s of states to only 10s of states, 2. increases the noise tolerance to the device conductance modulations by about 100x, and 3. increases the noise tolerance to the matrix-vector multiplication performed by the analog arrays by about 10x. Empirical simulation results show that TTv2 can train various neural networks close to their ideal accuracy even at extremely noisy hardware settings. TTv2 achieves these capabilities by complementing the original Tiki-Taka algorithm with lightweight and low computational complexity digital filtering operations performed outside the analog arrays. Therefore, the implementation cost of TTv2 compared to SGD and Tiki-Taka is minimal, and it maintains the usual power and speed benefits of using analog hardware for training workloads. Here we also show how to extract the neural network from the analog hardware once the training is complete for further model deployment. Similar to Bayesian model averaging, we form analog hardware compatible averages over the neural network weights derived from TTv2 iterates. This model average then can be transferred to another analog or digital hardware with notable improvements in test accuracy, transcending the trained model itself. In short, we describe an end-to-end training and model extraction technique for extremely noisy crossbar-based analog hardware that can be used to accelerate DNN training workloads and match the performance of full-precision SGD.
Science Diplomacy for Climate Action and Sustainable Development in Latin America and the Caribbean: How Important Is the Early Career Perspective to New Governance?
Authors: Cuellar-Ramirez Palmira
Published: September 2021

Science diplomacy and science–policy interfaces are tools that science has to address the biggest challenges that the world faces today. The scientific community needs to develop the capacity to bring this scientific knowledge to society and decision-makers for the purposes of new governance of the Earth System and thus a more resilient society. Climate change is one of the most challenging issues the world is currently facing, and the Latin America and Caribbean (LAC) region is highly vulnerable to its consequences, as it is expected to exacerbate environmental, social, and economic problems in the LAC region. In this context, and as an emergency call to address the climate crisis with the latest available science in the region, this paper collects a series of examples of the progress, best practices, gaps, challenges, and solutions. We do so from the perspective of Early Careers Researchers (ECRs) and undergraduate and graduate students, highlighting what we are doing to engage scientists in society–policy–science interaction for the sustainable development agenda and climate action in Latin America and the Caribbean.
Motion Polytopes in Virtual Reality for Shared Control in Remote Manipulation Applications
Authors: Zolotas Mark , Wonsick Murphy , Long Philip , Padır Taşkın
Published: September 2021

In remote applications that mandate human supervision, shared control can prove vital by establishing a harmonious balance between the high-level cognition of a user and the low-level autonomy of a robot. Though in practice, achieving this balance is a challenging endeavor that largely depends on whether the operator effectively interprets the underlying shared control. Inspired by recent works on using immersive technologies to expose the internal shared control, we develop a virtual reality system to visually guide human-in-the-loop manipulation. Our implementation of shared control teleoperation employs end effector manipulability polytopes, which are geometrical constructs that embed joint limit and environmental constraints. These constructs capture a holistic view of the constrained manipulator’s motion and can thus be visually represented as feedback for users on their operable space of movement. To assess the efficacy of our proposed approach, we consider a teleoperation task where users manipulate a screwdriver attached to a robotic arm’s end effector. A pilot study with prospective operators is first conducted to discern which graphical cues and virtual reality setup are most preferable. Feedback from this study informs the final design of our virtual reality system, which is subsequently evaluated in the actual screwdriver teleoperation experiment. Our experimental findings support the utility of using polytopes for shared control teleoperation, but hint at the need for longer-term studies to garner their full benefits as virtual guides.
Oral Spermine Supplementation in Gestated Rabbit: A Study on Villi Height of Immature Intestines
Authors: Tamba Riana Pauline , Moenadjat Yefta
Published: September 2021

Introduction: Immature intestines are the major problem in prematurity. Postnatal oral spermine has been shown in studies to improve intestinal maturation in rats and piglets. This study aimed to find out the efficacy of spermine in rabbits during gestation.

Method: An experimental study was done in an unblinded, randomized manner on those treated with and without spermine administration. A morphological examination of hematoxylin–eosin-stained villi was performed under a light microscope with a focus on villi height. Data were subjected to analysis.

Results: The median of the spermine-treated group was found to be higher at 24, 26, and 28 days than the non-spermine group, but was not significantly different.

Conclusion: Oral spermine supplementation during gestation might improve intestinal villi height in immature rabbit intestines.
Antimicrobial Activity and Composition of Five Rosmarinus (Now Salvia spp. and Varieties) Essential Oils
Authors: Pieracci Ylenia , Ciccarelli Daniela , Giovanelli Silvia , Pistelli Luisa , Flamini Guido , Cervelli Claudio , Mancianti Francesca , Nardoni Simona , Bertelloni Fabrizio , Ebani Valentina Virginia
Published: September 2021

Salvia rosmarinus Spenn. and Salvia jordanii J.B.Walker are aromatic evergreen shrubs belonging to the Lamiaceae family. Their aerial parts have been used since ancient times as natural preservatives. The present study reported the investigation of the chemical profile and the extraction yield of the essential oils (EOs) obtained from the dried aerial parts of four cultivars of Salvia rosmarinus (‘Boule’; ‘Vicomte de Noailles’; ‘Gorizia’; ‘Joyce de Baggio’) and the species S. jordanii, together with their antibacterial and antifungal activities. The phytochemical investigation evidenced a predominance of oxygenated monoterpenes in all the samples (57.5–77.1%), except in ‘Boule’, in which the hydrocarbon form prevailed (50.2%). Principal Component Analysis (PCA) of the matrix taxa × compounds showed that nine compounds have a significant discriminating function between the samples. ‘Vicomte de Noailles’ was characterized by high amounts of camphor and 14-hydroxy-9-epi-(E)-caryophyllene, while ‘Gorizia’ and Jord differed in their predominance of camphene, borneol, bornyl acetate, and α-humulene. Lastly, ‘Boule’ and ‘Joyce de Baggio’ segregated separately and were characterized by high amounts of α-pinene, myrcene, and verbenone. The selected EOs presented a moderate antibacterial activity on the tested bacterial strains and resulted not active on the tested yeast species.
Analgesic and Anti-Inflammatory Effects of the Synthetic Neurosteroid Analogue BNN27 during CFA-Induced Hyperalgesia
Authors: Poulaki Smaragda , Rassouli Olga , Liapakis George , Gravanis Achille , Venihaki Maria
Published: September 2021

Dehydroepiandrosterone (DHEA), an adrenal and neurosteroid hormone with strong neuroprotective and immunomodulatory properties, and ligand for all high-affinity neurotrophin tyrosine kinase receptors (Trk), also exerts important effects on hyperalgesia. Its synthetic, 17-spiro-epoxy analogue, BNN27, cannot be converted to estrogen or androgen as DHEA; it is a specific agonist of TrkA, the receptor of pain regulator Nerve Growth Factor (NGF), and it conserves the immunomodulatory properties of DHEA. Our study aimed to evaluate the anti-nociceptive and anti-inflammatory properties of BNN27 during Complete Freund’s Adjuvant (CFA)-induced inflammatory hyperalgesia in mice. Hyperalgesia was evaluated using the Hargreaves test. Inflammatory markers such as cytokines, NGF and opioids were measured, additionally to corticosterone and the protein kinase B (AKT) signaling pathway. We showed for the first time that treatment with BNN27 reversed hyperalgesia produced by CFA. The effect of BNN27 involved the inhibition of NGF in the dorsal root ganglia (DRG) and the increased synthesis of opioid peptides and their receptors in the inflamed paw. We also found alterations in the cytokine levels as well as in the phosphorylation of AKT2. Our findings strongly support that BNN27 represents a lead molecule for the development of analgesic and anti-inflammatory compounds with potential therapeutic applications in inflammatory hyperalgesia.
A Bio-Fluorometric Acetone Gas Imaging System for the Dynamic Analysis of Lipid Metabolism in Human Breath
Authors: Arakawa Takahiro , Mizukoshi Naoki , Iitani Kenta , Toma Koji , Mitsubayashi Kohji
Published: September 2021

We constructed an imaging system to measure the concentration of acetone gas by acetone reduction using secondary alcohol dehydrogenase (S-ADH). Reduced nicotinamide adenine dinucleotide (NADH) was used as an electron donor, and acetone was imaged by fluorescence detection of the decrease in the autofluorescence of NADH. In this system, S-ADH–immobilized membranes wetted with buffer solution containing NADH were placed in a dark box, and UV-LED excitation sheets and a high-sensitivity camera were installed on both sides of the optical axis to enable loading of acetone gas. A hydrophilic polytetrafluoroethylene (H-PTFE) membrane with low autofluorescence was used as a substrate, and honeycomb-like through-hole structures were fabricated using a CO2 laser device. After loading the enzyme membrane with acetone gas standards, a decrease in fluorescence intensity was observed in accordance with the concentration of acetone gas. The degree of decrease in fluorescence intensity was calculated using image analysis software; it was possible to quantify acetone gas at concentrations of 50–2000 ppb, a range that includes the exhaled breath concentration of acetone in healthy subjects. We applied this imaging system to measure the acetone gas in the air exhaled by a healthy individual during fasting.
Test of Motor Proficiency Second Edition (BOT-2) Short Form: A Systematic Review of Studies Conducted in Healthy Children
Authors: Radanović Danilo , Đorđević Dušan , Stanković Mima , Pekas Damir , Bogataj Špela , Trajkovic Nebojša
Published: September 2021

Motor skill competence of children is one of the important predictors of health because if a child is physically active during early childhood, the possibility of occurrence of many chronic diseases in adulthood will be reduced. The aim of this study was to systematically review the studies conducted in healthy children using the shorter form of the Bruininks-Oseretsky (BOT-2) and to determine the applicability in cross-sectional studies and pre-post designs. The search and analysis of the studies were done in accordance with the PRISMA guidelines. An electronic databases search (Google Scholar, PubMed, Mendeley, Science Direct, and Scopus) yielded 250 relevant studies conducted from 2011 to 2020. A total of 21 studies were included in quantitative synthesis, with a total of 3893 participants, both male and female. Through this study, the BOT-2 test proved its broad applicability, so it can be concluded that this test can be used to improve motor proficiency in a healthy population of children. Hence, it is necessary to invest a lot of time during the implementation of various programs so that children would adequately develop their basic motor skills so they broaden their own repertoire of movements.
Dural Sinus Arteriovenous Malformation in the Fetus. Case Report and Discussion of the Literature
Authors: Fesslova Vlasta , Colli Anna Maria , Boito Simona , Fabietti Isabella , Triulzi Fabio , Persico Nicola
Published: September 2021

Sonographic findings of cerebral arteriovenous malformations in the fetus are uncommon and usually regard aneurysm of the Galen vein. Outcome of arteriovenous malformations is usually severe. We report a case of a fetus at 21 weeks’ gestation with a rarer arteriovenous malformation, referred to us for echocardiography on account of a suspicious cardiomegaly at obstetrical scan. Upon examination, we found cardiomegaly, together with an associated moderate tricuspid regurgitation, however, there were no clear features of tricuspid dysplasia. Considering an unusually dilated superior vena cava, we found via color Doppler imaging a systodiastolic flow at Color Doppler progressing. Subsequent MRI of the central nervous system determined the localization in the sinus dura mater. Due to an already evident hemodynamic impact, the parents opted for the termination of the pregnancy. Autopsy confirmed a voluminous arteriovenous malformation of the transverse sinus of the dura mater, severe cardiomegaly, mainly of the ventricles, and hypoplasia of the lungs.
Wigner’s Friend Scenarios and the Internal Consistency of Standard Quantum Mechanics
Authors: Sokolovski Dmitri , Matzkin Alexandre
Published: September 2021

Wigner’s friend scenarios involve an Observer, or Observers, measuring a Friend, or Friends, who themselves make quantum measurements. In recent discussions, it has been suggested that quantum mechanics may not always be able to provide a consistent account of a situation involving two Observers and two Friends. We investigate this problem by invoking the basic rules of quantum mechanics as outlined by Feynman in the well-known “Feynman Lectures on Physics”. We show here that these “Feynman rules” constrain the a priori assumptions which can be made in generalised Wigner’s friend scenarios, because the existence of the probabilities of interest ultimately depends on the availability of physical evidence (material records) of the system’s past. With these constraints obeyed, a non-ambiguous and consistent account of all measurement outcomes is obtained for all agents, taking part in various Wigner’s Friend scenarios.
A Satellite Incipient Fault Detection Method Based on Decomposed Kullback–Leibler Divergence
Authors: Zhang Ge , Yang Qiong , Li Guotong , Leng Jiaxing , Yan Mubiao
Published: September 2021

Detection of faults at the incipient stage is critical to improving the availability and continuity of satellite services. The application of a local optimum projection vector and the Kullback–Leibler (KL) divergence can improve the detection rate of incipient faults. However, this suffers from the problem of high time complexity. We propose decomposing the KL divergence in the original optimization model and applying the property of the generalized Rayleigh quotient to reduce time complexity. Additionally, we establish two distribution models for subfunctions F1(w) and F3(w) to detect the slight anomalous behavior of the mean and covariance. The effectiveness of the proposed method was verified through a numerical simulation case and a real satellite fault case. The results demonstrate the advantages of low computational complexity and high sensitivity to incipient faults.
Educational Hall Escape: Increasing Motivation and Raising Emotions in Higher Education Students
Authors: Macías-Guillén Almudena , Díez Raquel Montes , Serrano-Luján Lucía , Borrás-Gené Oriol
Published: September 2021

Educational Escape Room is an innovative method used in classrooms to motivate students. This article describes a version of Educational Escape Room applied to undergraduate students. Specifically, this work presents an adaptation of the method called Educational Hall Escape, characterized by the resolution of challenges in a game-adapted room in which several student groups compete to finish the activity in the least amount of time. To date, the Educational Hall Escape method applied to the field of business economy has not been reported in the literature. The objective of the study is to analyze the influence of the Educational Hall Escape method on the learning processes and emotions of students during the activity and its impact on their motivation and the reinforcement their competences and knowledge. An experiment was designed in which the class was divided into a control group and an experimental group. To measure the impact of the experience in the students, two tools were used: an exam and the Gamefulquest survey. Despite the fact that the results obtained show that the students perceived the experience as a game, it improved their motivation and increased their proclivity to have an emotional bond with the subject, the academic results remained steady.
Bioenergy: A Sustainable Shift
Authors: D’Adamo Idiano , Morone Piergiuseppe , Huisingh Donald
Published: September 2021

The European Commission emphasised that a bioeconomy is an economy that uses renewable biological resources from the land and sea (e [...]
Numerical Study of the Influence of Secondary Air Uniformity on Jet Penetration and Gas-Solid Diffusion Characteristics in a Large-Scale CFB Boiler
Authors: Zheng Xiong , Yan Jin , Wang Jinping , Lu Xiaofeng
Published: September 2021

The uniformity of secondary air (SA) in large-scale CFB boilers has an important influence on gas-solid flow and combustion, but was seldom considered in previous studies. Numerical simulation based on the Eulerian–Eulerian and RNG k-ε turbulence models was conducted to explore the influence of SA uniformity and load variation on jet penetration, diffusion characteristics and gas-solid mixing in the first 600 MW supercritical CFB boiler. The results showed that better SA uniformity was conductive to the uniformity of SA penetration and gas-solid mixing along the furnace height, although the penetration depth and diffusion distance showed an opposite trend. In addition, the penetration depth and diffusion distance got enhanced with higher boiler load. The inner and outer SA jets could not cover the furnace width, and the uneven SA uniformity led to a huge deviation of the solid concentration within 10 m of the air distributor. Eventually, a calculation model was successfully established for predicting the penetration depth of inclined thermal SA jets during boiler operation.
Spatial–Temporal Patterns of Historical, Near-Term, and Projected Drought in the Conterminous United States
Authors: Kotikot Susan M. , Omitaomu Olufemi A.
Published: September 2021

Major droughts in the United States have heavily impacted the hydrologic system, negatively effecting energy and food production. Improved understanding of historical drought is critical for accurate forecasts. Data from global climate models (GCMs), commonly used to assess drought, cannot effectively evaluate local patterns because of their low spatial scale. This research leverages downscaled (~4 km grid spacing) temperature and precipitation estimates from nine GCMs’ data under the business-as-usual scenario (Representative Concentration Pathway 8.5) to examine drought patterns. Drought severity is estimated using the Palmer Drought Severity Index (PDSI) with the Thornthwaite evapotranspiration method. The specific objectives were (1) To reproduce historical (1966–2005) drought and calculate near-term to future (2011–2050) drought patterns over the conterminous USA. (2) To uncover the local variability of spatial drought patterns in California between 2012 and 2018 using a network-based approach. Our estimates of land proportions affected by drought agree with the known historical drought events of the mid-1960s, late 1970s to early 1980s, early 2000s, and between 2012 and 2015. Network analysis showed heterogeneity in spatial drought patterns in California, indicating local variability of drought occurrence. The high spatial scale at which the analysis was performed allowed us to uncover significant local differences in drought patterns. This is critical for highlighting possible weak systems that could inform adaptation strategies such as in the energy and agricultural sectors.
Spatial Modeling of COVID-19 Vaccine Hesitancy in the United States
Authors: Mollalo Abolfazl , Tatar Moosa
Published: September 2021

Vaccine hesitancy refers to delay in acceptance or refusal of vaccines despite the availability of vaccine services. Despite the efforts of United States healthcare providers to vaccinate the bulk of its population, vaccine hesitancy is still a severe challenge that has led to the resurgence of COVID-19 cases to over 100,000 people during early August 2021. To our knowledge, there are limited nationwide studies that examined the spatial distribution of vaccination rates, mainly based on the social vulnerability index (SVI). In this study, we compiled a database of the percentage of fully vaccinated people at the county scale across the continental United States as of 29 July 2021, along with SVI data as potential significant covariates. We further employed multiscale geographically weighted regression to model spatial nonstationarity of vaccination rates. Our findings indicated that the model could explain over 79% of the variance of vaccination rate based on Per capita income and Minority (%) (with positive impacts), and Age 17 and younger (%), Mobile homes (%), and Uninsured people (%) (with negative effects). However, the impact of each covariate varied for different counties due to using separate optimal bandwidths. This timely study can serve as a geospatial reference to support public health decision-makers in forming region-specific policies in monitoring vaccination programs from a geographic perspective.
Associations between Movement Behaviours and Obesity Markers among Preschoolers Compliant and Non-Compliant with Sleep Duration: A Latent Profile Analysis
Authors: Souza Alesandra A. de , Mota Jorge A. P. S. , Silva Gustavo M. G. da , Tassitano Rafael M. , Clark Cain C. T. , Duncan Michael J. , Martins Clarice M. de L.
Published: September 2021

This study identifies physical activity (PA) and sedentary behaviour (SB) clusters in preschoolers compliant (C) or non-compliant (NC) with sleep recommendations; and associates these clusters with obesity markers. PA and SB were objectively assessed (Actigraph WGT3-X) in 272 preschoolers (4.4 ± 0.7 years old). Sleep duration was parent-reported, and preschoolers were classified as C (3–4 years old: 600–780 min/day; 5 years old: 540–660 min/day) or NC with sleep recommendations. Body mass index (BMI) and waist circumference (WC) were assessed according to international protocols. Moderate to vigorous physical activity (MVPA) and light physical activity (LPA) were categorized as low/high (<60 min/>60 min/day or <180 min/180 min/day, respectively). SB was defined according to mean values between clusters. Latent profile analysis was performed. Associations between the observed clusters and obesity markers were determined using linear regression (RStudio; 1.3.1073). Four cluster solutions for C and NC preschoolers were identified. A negative association between C/Low MVPA cluster and BMI, and a positive association between NC/Low MVPA and BMI (β = −0.8, 95%CI = −1.6;−0.1, and β = 0.9, 95%CI = 0.1;1.7, respectively) were observed. No association was seen for SB clusters. Adequate sleep duration may have a protective role for preschoolers’ BMI, even if the children do not comply with MVPA recommendations.
A System Dynamics Model for Urban Residential Building Stock towards Sustainability: The Case of Jinan, China
Authors: Yang Dong , Dang Mengyuan , Sun Lingwen , Han Feng , Shi Feng , Zhang Hongbo , Zhang Hongjun
Published: September 2021

Resource and environmental issues related to urban building systems have recently become a hot research topic in the field of urban environmental management research. Taking Jinan city as an example, this paper establishes a system dynamic model for an urban residential building stock system. The simulated results show that the urban residential building stock will be 1.99 × 108 m2 in 2050; and the annual total demolition buildings will be at 3.36 × 106 m2 in 2082. Policy measures were developed based on four important action fields such as per capita floor area (PCFA), the building structure proportion of new construction, lifetime of the residential building, and the recycling of the C&D waste. Among these approaches, the set of policy measures focusing on the recycling of C&D waste appears to be more effective in reducing environmental and resource impacts than the other three fields. It is also found that the recycling of brick and concrete waste plays a considerable role in reducing environment and resource impacts due to the development of urban residential building stock with the lapse of time.
Translation of the Weight-Related Behaviours Questionnaire into a Short-Form Psychosocial Assessment Tool for the Detection of Women at Risk of Excessive Gestational Weight Gain
Authors: Fealy Shanna , Leigh Lucy , Hazelton Michael , Attia John , Foureur Maralyn , Oldmeadow Christopher , Collins Clare E. , Smith Roger , Hure Alexis J.
Published: September 2021

The identification and measurement of psychosocial factors that are specific to pregnancy and relevant to gestational weight gain is a challenging task. Given the general lack of availability of pregnancy-specific psychosocial assessment instruments, the aim of this study was to develop a short-form psychosocial assessment tool for the detection of women at risk of excessive gestational weight gain with research and clinical practice applications. A staged scale reduction analysis of the weight-related behaviours questionnaire was conducted amongst a sample of 159 Australian pregnant women participating in the Women and Their Children’s Health (WATCH) pregnancy cohort study. Exploratory factor analysis, univariate logistic regression, and item response theory techniques were used to derive the minimum and most predictive questions for inclusion in the short-form assessment tool. Of the total 49 questionnaire items, 11 items, all 4 body image items, n = 4 attitudes towards weight gain, and n = 3 self-efficacy items, were retained as the strongest predictors of excessive gestational weight gain. These within-scale items were highly correlated, exhibiting high item information function value statistics, and were observed to have high probability (p < 0.05) for excessive gestational weight gain, in the univariate analysis. The short-form questionnaire may assist with the development of tailored health promotion interventions to support women psychologically and physiologically to optimise their pregnancy weight gain. Confirmatory factor analysis is now required.
International Multicenter Study on Drug Consumption in Nursing Students
Authors: Ponce-Blandón José Antonio , Martínez-Montilla José Manuel , Pabón-Carrasco Manuel , Martos-García Raúl , Castro-Méndez Aurora , Romero-Castillo Rocío
Published: September 2021

Background: The prevalence of illicit drug use among young people is high, with many being highly vulnerable to substance abuse. The nursing profession is not immune to the impacts of substance misuse. Knowing the current levels of consumption of illicit drugs in nursing students will allow for the introduction of preventive actions. Methods: Multi-center, descriptive, and cross-sectional study involving nursing schools from four different countries (Spain, Belgium, France, and Brazil). A total of seven centers participated. An adapted version of the Global School-based Student Health Survey (GSHS) was used as a tool, which selected only the module on illicit drugs. Standard logistic regression analysis was performed. Results: A total of 496 nursing students participated in the study. Illicit drug shows positive representation among nursing students. A significant difference was observed between the gender and the age of first drug use, illicit drug consumption, cannabis use, and cocaine use ever in life, with higher use of illicit drugs by male, although at later ages than girls. The bivariate analysis, gender, problems as result of drugs, and nationality were significantly associated with the consumption of illicit drugs, cannabis, cocaine, and ecstasy ever in life. Conclusions: High rates of illicit drug use were found among nursing students, as well as factors that can influence consumption such as nationality or gender. These results can serve as a basis for the development of educational and policy interventions within nursing schools that are based on evidence, with significant implications for nurse educators, academic administrators, and practice.
Open MHC Class I Conformers: A Look through the Looking Glass
Authors: Arosa Fernando A. , Esgalhado André J. , Reste-Ferreira Débora , Cardoso Elsa M.
Published: September 2021

Studies carried out during the last few decades have consistently shown that cell surface MHC class I (MHC-I) molecules are endowed with functions unrelated with antigen presentation. These include cis–trans-interactions with inhibitory and activating KIR and LILR, and cis-interactions with receptors for hormones, growth factors, cytokines, and neurotransmitters. The mounting body of evidence indicates that these non-immunological MHC-I functions impact clinical and biomedical settings, including autoimmune responses, tumor escape, transplantation, and neuronal development. Notably, most of these functions appear to rely on the presence in hematopoietic and non-hematopoietic cells of heavy chains not associated with β2m and the peptide at the plasma membrane; these are known as open MHC-I conformers. Nowadays, open conformers are viewed as functional cis-trans structures capable of establishing physical associations with themselves, with other surface receptors, and being shed into the extracellular milieu. We review past and recent developments, strengthening the view that open conformers are multifunctional structures capable of fine-tuning cell signaling, growth, differentiation, and cell communication.
Short- and Long-Term Study of the Impact of Focal Blue Light-Emitting Diode-Induced Phototoxicity in Adult Albino Rats
Authors: Miralles de Imperial-Ollero Juan A. , Gallego-Ortega Alejandro , Norte-Muñoz María , Di Pierdomenico Johnny , Bernal-Garro José Manuel , Valiente-Soriano Francisco J. , Vidal-Sanz Manuel
Published: September 2021

Background: In adult rats we study the short- and long-term effects of focal blue light-emitting diode (LED)-induced phototoxicity (LIP) on retinal thickness and Iba-1+ activation. Methods: The left eyes of previously dark-adapted Sprague Dawley (SD) rats were photoexposed to a blue LED (20 s, 200 lux). In vivo longitudinal monitoring of retinal thickness, fundus images, and optical retinal sections was performed from 1 to 30 days (d) after LIP with SD-OCT. Ex vivo, we analysed the population of S-cone and Iba-1+ cells within a predetermined fixed-size circular area (PCA) centred on the lesion. Results: LIP resulted in a circular focal lesion readily identifiable in vivo by fundus examination, which showed within the PCAs a progressive thinning of the outer retinal layer, and a diminution of the S-cone population to 19% by 30 d. In parallel to S-cone loss, activated Iba-1+ cells delineated the lesioned area and acquired an ameboid morphology with peak expression at 3 d after LIP. Iba-1+ cells adopted a more relaxed-branched morphology at 7 d and by 14–30 d their morphology was fully branched. Conclusion: LIP caused a progressive reduction of the outer retina with loss of S cones and a parallel dynamic activation of microglial cells in the lesioned area.
Aquaporin-4 Mediates Permanent Brain Alterations in a Mouse Model of Hypoxia-Aged Hydrocephalus
Authors: Trillo-Contreras José Luis , Toledo-Aral Juan José , Villadiego Javier , Echevarría Miriam
Published: September 2021

Aquaporin-4 (AQP4) is the principal water channel in the brain being expressed in astrocytes and ependymal cells. AQP4 plays an important role in cerebrospinal fluid (CSF) homeostasis, and alterations in its expression have been associated with hydrocephalus. AQP4 contributes to the development of hydrocephalus by hypoxia in aged mice, reproducing such principal characteristics of the disease. Here, we explore whether these alterations associated with the hydrocephalic state are permanent or can be reverted by reexposure to normoxia. Alterations such as ventriculomegaly, elevated intracranial pressure, and cognitive deficits were reversed, whereas deficits in CSF outflow and ventricular distensibility were not recovered, remaining impaired even one month after reestablishment of normoxia. Interestingly, in AQP4−/− mice, the impairment in CSF drainage and ventricular distensibility was completely reverted by re-normoxia, indicating that AQP4 has a structural role in the chronification of those alterations. Finally, we show that aged mice subjected to two hypoxic episodes experience permanent ventriculomegaly. These data reveal that repetitive hypoxic events in aged cerebral tissue promote the permanent alterations involved in hydrocephalic pathophysiology, which are dependent on AQP4 expression.
The Central PXXP Motif Is Crucial for PMAP-23 Translocation across the Lipid Bilayer
Authors: Yang Sung-Tae , Shin Song-Yub , Shin Sung-Heui
Published: September 2021

PMAP-23, a cathelicidin-derived host defense peptide, does not cause severe membrane permeabilization, but exerts strong and broad-spectrum bactericidal activity. We have previously shown that it forms an amphipathic α-helical structure with a central hinge induced by the PXXP motif, which is implicated in the interaction of PMAP-23 with negatively charged bacterial membranes. Here, we studied the potential roles of the PXXP motif in PMAP-23 translocation across the lipid bilayer by replacing Pro residues with either α-helix former Ala (PMAP-PA) or α-helix breaker Gly (PMAP-PG). Although both PMAP-PA and PMAP-PG led to effective membrane depolarization and permeabilization, they showed less antimicrobial activity than wild-type PMAP-23. Interestingly, we observed that PMAP-23 crossed lipid bilayers much more efficiently than its Pro-substituted derivatives. The fact that the Gly-induced hinge was unable to replace the PXXP motif in PMAP-23 translocation suggests that the PXXP motif has unique structural properties other than the central hinge. Surface plasmon resonance sensorgrams showed that the running buffer almost entirely dissociated PMAP-23 from the membrane surface, while its Pro-substituted derivatives remained significantly bound to the membrane. In addition, kinetic analysis of the sensorgrams revealed that the central PXXP motif allows PMAP-23 to rapidly translocate at the interface between the hydrophilic and hydrophobic phases. Taken together, we propose that the structural and kinetic understanding of the PXXP motif in peptide translocation could greatly aid the development of novel antimicrobial peptides with intracellular targets by promoting peptide entry into bacterial cells.
Synaptic Dysfunction in Multiple Sclerosis: A Red Thread from Inflammation to Network Disconnection
Authors: Bellingacci Laura , Mancini Andrea , Gaetani Lorenzo , Tozzi Alessandro , Parnetti Lucilla , Di Filippo Massimiliano
Published: September 2021

Multiple sclerosis (MS) has been clinically considered a chronic inflammatory disease of the white matter; however, in the last decade growing evidence supported an important role of gray matter pathology as a major contributor of MS-related disability and the involvement of synaptic structures assumed a key role in the pathophysiology of the disease. Synaptic contacts are considered central units in the information flow, involved in synaptic transmission and plasticity, critical processes for the shaping and functioning of brain networks. During the course of MS, the immune system and its diffusible mediators interact with synaptic structures leading to changes in their structure and function, influencing brain network dynamics. The purpose of this review is to provide an overview of the existing literature on synaptic involvement during experimental and human MS, in order to understand the mechanisms by which synaptic failure eventually leads to brain networks alterations and contributes to disabling MS symptoms and disease progression.
Activation of Coronary Arteriolar PKCβ2 Impairs Endothelial NO-Mediated Vasodilation: Role of JNK/Rho Kinase Signaling and Xanthine Oxidase Activation
Authors: Thengchaisri Naris , Hein Travis , Ren Yi , Kuo Lih
Published: September 2021

Protein kinase C (PKC) activation can evoke vasoconstriction and contribute to coronary disease. However, it is unclear whether PKC activation, without activating the contractile machinery, can lead to coronary arteriolar dysfunction. The vasoconstriction induced by the PKC activator phorbol 12,13-dibutyrate (PDBu) was examined in isolated porcine coronary arterioles. The PDBu-evoked vasoconstriction was sensitive to a broad-spectrum PKC inhibitor but not affected by inhibiting PKCβ2 or Rho kinase. After exposure of the vessels to a sub-vasomotor concentration of PDBu (1 nmol/L, 60 min), the endothelium-dependent nitric oxide (NO)-mediated dilations in response to serotonin and adenosine were compromised but the dilation induced by the NO donor sodium nitroprusside was unaltered. PDBu elevated superoxide production, which was blocked by the superoxide scavenger Tempol. The impaired NO-mediated vasodilations were reversed by Tempol or inhibition of PKCβ2, xanthine oxidase, c-Jun N-terminal kinase (JNK) and Rho kinase but were not affected by a hydrogen peroxide scavenger or inhibitors of NAD(P)H oxidase and p38 kinase. The PKCβ2 protein was detected in the arteriolar wall and co-localized with endothelial NO synthase. In conclusion, activation of PKCβ2 appears to compromise NO-mediated vasodilation via Rho kinase-mediated JNK signaling and superoxide production from xanthine oxidase, independent of the activation of the smooth muscle contractile machinery.
Left Ventricular Myocardial Noncompaction with Advanced Atrioventricular Conduction Disorder and Ventricular Arrhythmias in a Young Patient: Role of MIB1 Gene
Authors: Balla Cristina , De Raffele Martina , Deserio Maria Angela , Sanchini Mariabeatrice , Farnè Marianna , Trabanelli Cecilia , Ragni Luca , Biffi Mauro , Ferlini Alessandra , Rapezzi Claudio , Gualandi Francesca , Bertini Matteo
Published: September 2021

Left ventricular noncompaction (LVNC) is a structural abnormality of the left ventricle, usually described as an isolated condition, or sometimes associated with other structural cardiac diseases. LVNC is generally asymptomatic, although it may present conduction disorders, arrhythmias, and heart failure. Here, we present the case of a patient who came to our attention with a severe LVNC phenotype associated with advanced AV conduction disorder, and supraventricular and ventricular arrhythmias at young age, in which a novel MIB1, likely pathogenic, variation has been identified.
Financial Market Reaction to Patent Lawsuits against Integrated Circuit Design Companies
Authors: Yu Su-Chen , Shih Kuang-Hsun
Published: September 2021

With the rapid advancement in technology, Taiwan’s integrated circuit (IC) design companies have made a mark in the international semiconductor industry but are unable to independently develop the key core technologies they need. Therefore, strategic alliances, competition and cooperation have become a means for enterprises to quickly obtain patents and capture the market. However, listed companies upstream and downstream of Taiwan’s supply chain have been facing patent infringement lawsuits in recent years. This research mainly aims to provide investors with investment strategies when companies face patent litigation, analyze the abnormal returns on the underlying stocks through the event research method, and use the cross-sectional multiple regression model to explore the changes in different factors based on the results. The empirical results show that positive abnormal returns are generated before and after a company faces patent litigation and the cumulative abnormal rewards are all positive and significant after the incident, which indicates that the company may still have an opportunity to make a profit when facing patent litigation, which can be used as a reference for investors.
Opportunities and Challenges of the European Green Deal for the Chemical Industry: An Approach Measuring Innovations in Bioeconomy
Authors: Thormann Lisa , Neuling Ulf , Kaltschmitt Martin
Published: September 2021

The Circular Economy Action Plan, as part of the European Green Deal announced by the European Commission, is highly relevant to the chemical industry in relation to the production of sustainable products. Accordingly, the chemical industry faces the question of how far it can promote its own manufacture of sustainable products. Within this context, this article presents an approach on how to measure innovations in bioeconomy. The methodological framework developed provides the chemical industry with an approach to assess the effectiveness of innovative conversion technologies producing biogenic intermediate products (e.g., bulk chemicals). The innovations within the bioeconomy (TRL > 4; TRL—technology readiness level) are compared in terms of technical, economic, and environmental indicators for the current status, for the medium- and long-term as well as for different production sites. The methodological approach developed here is exemplarily applied, assessing the production of intermediate biogenic products via thermo-chemical conversion of lignocellulosic biomass. The results show the successful applicability of the developed assessment approach as well as significant differences in efficiency, costs, and environmental impact, both from the perspective of time and in spatial terms within the European Union. Thus, the methodological approach developed and presented enables the chemical industry to reduce challenges and to take advantage of the opportunities arising from the transition to a climate-neutral and circular economy.