bluuebunny/crossref_metadata_2025 huggingface dataset:
The incidence and interrelationship of hemivertebra and concomitant cardiac abnormalities in congenital scoliosis
Authors: Wang Shengru , Zhao Yiwei , Du You , Zhang Jianguo , Yu Bin
Published: July 2023

Background

Congenital scoliosis(CS) is associated with multiple organs defect, and cardiac abnormalities have been reported commonly associated with CS. Hemivertebra is caused by the failure of vertebral formation, which is a major constitute of CS. Till now, few studies focus on the incidence and interrelationship of hemivertebra and concomitant cardiac abnormalities in congenital scoliosis. We aimed to analyze the cardiac defect in CS patients with or without hemivertebra, and further explore the incidence of cardiac defect between different types of hemivertebra.

Methods

The ultrasonic cardiography (UCG) results of surgically treated congenital scoliosis (CS) patients between 2015 and 2018 were retrospectively analyzed. Patients were divided into hemivertebra group and non-hemivertebra group according to preoperative CT. Patients with hemivertebra was further divided into sub-group by single/multiple or fully/partially/mixed segmented hemivertebra. Demographic information, radiographic data and cardiac abnormalities were statistically compared between groups.

Results

A total of 329 patients were analyzed, including 216 patients with hemivertebra and 113 patients without hemivertebra. UCG results were abnormal in 89 cases (27.1%), including 41 males(12.5%) and 48 females(14.6%). Hemivertebra group had comparable incidence of cardiac abnormalities with non-hemivertebra group (p = 0.517). No significant difference in the incidence of UCG abnormalities between single and multiple hemivertebra group (P = 0.246). Binary logistic regression analysis showed that female sex with multiple hemivertebra was a risk factor for abnormal UCG (P = 0.009, OR = 3.449). Cardiac abnormalities was comparable among fully, partially and mixed segmented hemivertebra group(P = 0.264). In abnormal UCG, 33 patients with hemivertebra had non-valvular abnormalities, and 48.5% (16/33) were septal defects. 28 patients had valvular abnormalities, most of them were mitral valve abnormalities, especially mitral valve redundancy, prolapse and insufficiency(82.1%, 23/28). No significant difference between the incidence of non-valvular and valvular abnormalities in patients with hemivertebra (P = 0.581).

Conclusions

The incidence of abnormal UCG results was approximately 28.2% in CS patients with hemivertebra. Female patients with multiple hemivertebra had a higher risk of UCG abnormalities. Mitral valve abnormalities were the most common abnormality of UCG found in CS patients with hemivertebra.

Trial registration

retrospectively registered.
Multiorgan failure caused by Chinese herbal medicine Xanthii Fructus poisoning: a case report
Authors: Li Yaqian , Yu Guangcai , Shi Longke , Zhao Liwen , Wen Zixin , Kan Baotian , Jian Xiangdong
Published: July 2023

Background

Xanthii Fructus was used in the treatment of rhinitis and related nasal disease. It is the most commonly used chemically active component in compounds formulated for the treatment of rhinitis. However, poisoning, resulting in serious consequences, can easily occur owing to cocklebur overdose, improper processing, or usage without processing.

Case presentation

We reported on a 55-year-old man who experienced allergic rhinitis for 2.5 years. He ingested unprocessed Xanthii Fructus for 2 months as treatment. However, he developed anorexia; nausea; abdominal pain; general weakness; hiccups; oliguria and anuria; significantly elevated serum alanine aminotransferase, aspartate aminotransferase, and creatinine levels; and abnormalities in blood coagulation series. Nutritional support; daily drugs for liver protection, gastric protection, inflammation reduction; fresh plasma; and cryoprecipitate infusion were administered. Continuous venovenous hemodialysis (Prismaflex ST100) was also administered. However, the patient’s multiple organ failure gradually worsened, ultimately leading to death.

Conclusion

Xanthii Fructus poisoning affects multiple systems, and its clinical manifestations are complex. Therefore, it is easily misdiagnosed and missed. Along with careful inquiry of medical and medication history, early diagnosis and intervention are vital for a successful treatment. It is also important to educate people and create awareness about this poisoning. Therefore, this intractable case has great clinical significance.
Population transcriptogenomics highlights impaired metabolism and small population sizes in tree frogs living in the Chernobyl Exclusion Zone
Authors: Car Clément , Gilles André , Goujon Elen , Muller Marie-Laure Delignette , Camoin Luc , Frelon Sandrine , Burraco Pablo , Granjeaud Samuel , Baudelet Emilie , Audebert Stéphane , Orizaola Germán , Armengaud Jean , Tenenhaus Arthur , Garali Imène , Bonzom Jean-Marc , Armant Olivier
Published: July 2023

Background

Individual functional modifications shape the ability of wildlife populations to cope with anthropogenic environmental changes. But instead of adaptive response, human-altered environments can generate a succession of deleterious functional changes leading to the extinction of the population. To study how persistent anthropogenic changes impacted local species’ population status, we characterised population structure, genetic diversity and individual response of gene expression in the tree frog Hyla orientalis along a gradient of radioactive contamination around the Chernobyl nuclear power plant.

Results

We detected lower effective population size in populations most exposed to ionizing radiation in the Chernobyl Exclusion Zone that is not compensated by migrations from surrounding areas. We also highlighted a decreased body condition of frogs living in the most contaminated area, a distinctive transcriptomics signature and stop-gained mutations in genes involved in energy metabolism. While the association with dose will remain correlational until further experiments, a body of evidence suggests the direct or indirect involvement of radiation exposure in these changes.

Conclusions

Despite ongoing migration and lower total dose rates absorbed than at the time of the accident, our results demonstrate that Hyla orientalis specimens living in the Chernobyl Exclusion Zone are still undergoing deleterious changes, emphasizing the long-term impacts of the nuclear disaster.
Genome-wide chromatin accessibility landscape and dynamics of transcription factor networks during ovule and fiber development in cotton
Authors: Bao Yu , Wei Yangyang , Liu Yuling , Gao Jingjing , Cheng Shuang , Liu Guanqing , You Qi , Liu Peng , Lu Quanwei , Li Pengtao , Zhang Shulin , Hu Nan , Han Yangshuo , Liu Shuo , Wu Yuechao , Yang Qingqing , Li Zhaoguo , Ao Guowei , Liu Fang , Wang Kunbo , Jiang Jiming , Zhang Tao , Zhang Wenli , Peng Renhai
Published: July 2023

Background

The development of cotton fiber is regulated by the orchestrated binding of regulatory proteins tocis-regulatory elements associated with developmental genes. Thecis–transregulatory dynamics occurred throughout the course of cotton fiber development are elusive. Here we generated genome-wide high-resolution DNase I hypersensitive sites (DHSs) maps to understand the regulatory mechanisms of cotton ovule and fiber development.

Results

We generated DNase I hypersensitive site (DHS) profiles from cotton ovules at 0 and 3 days post anthesis (DPA) and fibers at 8, 12, 15, and 18 DPA. We obtained a total of 1185 million reads and identified a total of 199,351 DHSs through ~ 30% unique mapping reads. It should be noted that more than half of DNase-seq reads mapped multiple genome locations and were not analyzed in order to achieve a high specificity of peak profile and to avoid bias from repetitive genomic regions. Distinct chromatin accessibilities were observed in the ovules (0 and 3 DPA) compared to the fiber elongation stages (8, 12, 15, and 18 DPA). Besides, the chromatin accessibility during ovules was particularly elevated in genomic regions enriched with transposable elements (TEs) and genes in TE-enriched regions were involved in ovule cell division. We analyzedcis-regulatory modules and revealed the influence of hormones on fiber development from the regulatory divergence of transcription factor (TF) motifs. Finally, we constructed a reliable regulatory network of TFs related to ovule and fiber development based on chromatin accessibility and gene co-expression network. From this network, we discovered a novel TF, WRKY46, which may shape fiber development by regulating the lignin content.

Conclusions

Our results not only reveal the contribution of TEs in fiber development, but also predict and validate the TFs related to fiber development, which will benefit the research of cotton fiber molecular breeding.
The cardio-renal-metabolic connection: a review of the evidence
Authors: Marassi Marella , Fadini Gian Paolo
Published: July 2023

Type 2 diabetes (T2D), cardiovascular disease (CVD) and chronic kidney disease (CKD), are recognized among the most disruptive public health issues of the current century. A large body of evidence from epidemiological and clinical research supports the existence of a strong interconnection between these conditions, such that the unifying term cardio-metabolic-renal (CMR) disease has been defined. This coexistence has remarkable epidemiological, pathophysiologic, and prognostic implications. The mechanisms of hyperglycemia-induced damage to the cardio-renal system are well validated, as are those that tie cardiac and renal disease together. Yet, it remains controversial how and to what extent CVD and CKD can promote metabolic dysregulation. The aim of this review is to recapitulate the epidemiology of the CMR connections; to discuss the well-established, as well as the putative and emerging mechanisms implicated in the interplay among these three entities; and to provide a pathophysiological background for an integrated therapeutic intervention aiming at interrupting this vicious crosstalks.
SALL4 promotes angiogenesis in gastric cancer by regulating VEGF expression and targeting SALL4/VEGF pathway inhibits cancer progression
Authors: Abouelnazar Fatma A. , Zhang Xiaoxin , Zhang Jiahui , Wang Maoye , Yu Dan , Zang Xueyan , Zhang Jiayin , Li Yixin , Xu Jing , Yang Qiurong , Zhou Yue , Tang Haozhou , Wang Yanzheng , Gu Jianmei , Zhang Xu
Published: July 2023

Background

Spalt-like protein 4 (SALL4) is a stemness-related transcription factor whose abnormal re-expression contributes to cancer initiation and progression. However, the role of SALL4 in cancer angiogenesis remains unknown.

Methods

Analyses of clinical specimens via TCGA datasets were performed to determine the expression level and clinical significance of SALL4 in STAD (Stomach Adenocarcinoma). SALL4 knockdown, knockout, and overexpression were achieved by siRNA, CRISPR/Cas9, and plasmid transfection. The effects of conditioned medium (CM) from SALL4 knockdown or overexpression of gastric cancer cells on endothelial cell proliferation, migration, and tube formation were investigated by CCK-8 assay, transwell migration assay, and tube formation assay. The regulation of VEGF gene expression by SALL4 was studied by qRT-PCR, western blot, chromatin immunoprecipitation (ChIP) assay, and electrophoretic mobility shift assay (EMSA). Engineered exosomes from 293T cells loaded with si-SALL4-B and thalidomide were produced to test their therapeutic effect on gastric cancer progression.

Results

SALL4 expression was increased in STAD and positively correlated with tumor progression and poor prognosis. SALL4-B knockdown or knockout decreased while over-expression increased the promotion of human umbilical vein endothelial cells (HUVEC) cell proliferation, migration, and tube formation by gastric cancer cell-derived CM. Further investigation revealed a widespread association of SALL4 with angiogenic gene transcription through the TCGA datasets. Additionally, SALL4-B knockdown reduced, while over-expression enhanced the expression levels of VEGF-A, B, and C genes. The results of ChIP and EMSA assays indicated that SALL4 could directly bind to the promoters of VEGF-A, B, and C genes and activate their transcription, which may be associated with increased histone H3-K79 and H3-K4 modifications in their promoter regions. Furthermore, si-SALL4-B and thalidomide-loaded exosomes could be efficiently uptaken by gastric cancer cells and significantly reduced SALL4-B and Vascular Endothelial Growth Factor (VEGF) expression levels in gastric cancer cells, thus inhibiting the pro-angiogenic role of their derived CM.

Conclusion

These findings suggest that SALL4 plays an important role in angiogenesis by transcriptionally regulating VEGF expression. Co-delivery of the functional siRNA and anticancer drug via exosomes represents a useful approach to inhibiting cancer angiogenesis by targeting SALL4/VEGF pathway.
Biological reconstruction of bone defect after resection of malignant bone tumor by allograft: a single-center retrospective cohort study
Authors: Liu Qing , Long Feng , Zhang Can , Liu Yupeng , He Hongbo , Luo Wei
Published: July 2023

Background

Allograft reconstruction following the resection of malignant bone tumors is associated with high rates of complications and failures. This study aimed to evaluate the efficacy and current problems of allograft reconstruction techniques to optimize treatment strategies at our center.

Materials and methods

Thirty-eight cases (16 men and 22 women), who were diagnosed with malignant bone tumors and had undergone allograft reconstruction, were recruited. Allograft was fixed by intramedullary nail, single steel plate, double plate, and intramedullary nail combined plate in 2, 4, 17, and 15 cases, respectively. Allograft union, local recurrence, and complications were assessed with clinical and radiological tests. Tumor grade was assessed using the Enneking staging of malignant bone tumors. Functional prognosis was evaluated by the Musculoskeletal Tumor Society (MSTS) scoring system.

Results

Intercalary and osteoarticular reconstructions were performed in 32 and 6 cases, respectively. Six patients underwent reoperation related to allograft complications, four patients had local recurrence, and three patients with allograft fracture underwent allograft removal. A total of eight host–donor junctions showed nonunion, including seven cases (18.4%) in diaphysis and one case (3.1%) in metaphysis (p < 0.01). Host rejection and secondary osteoarthritis occurred in nine and two cases, respectively. No deep infection and internal fixation device fracture occurred. The overall allograft survival rate was 81.6%. Postoperative MSTS score of patients with allograft survival was 26.8 ± 2.9, indicating a significant improvement as compared to their preoperative function.

Conclusions

Allograft represents an excellent choice for intercalary bone defects after malignant bone tumor resection. Robust internal fixation protection across the whole length of the allograft is an important prerequisite for the survival of the allograft, while multidimensional osteotomy, intramedullary cement reinforcement, and pedicled muscle flap transfer can effectively improve the survival rate and healing rate of the allograft.
Recent advances of exosomal circRNAs in cancer and their potential clinical applications
Authors: Yi Qian , Yue Jiaji , Liu Yang , Shi Houyin , Sun Wei , Feng Jianguo , Sun Weichao
Published: July 2023

Circular RNA (circRNA) is a type of non-coding RNA that forms a covalently closed, uninterrupted loop. The expression of circRNA differs among cell types and tissues, and various circRNAs are aberrantly expressed in a variety of diseases, including cancer. Aberrantly expressed circRNAs contribute to disease progression by acting as microRNA sponges, functional protein sponges, or novel templates for protein translation. Recent studies have shown that circRNAs are enriched in exosomes. Exosomes are spherical bilayer vesicles released by cells into extracellular spaces that mediate intercellular communication by delivering cargoes. These cargoes include metabolites, proteins, lipids, and RNA molecules. Exosome-mediated cell-cell or cell-microenvironment communications influence the progression of carcinogenesis by regulating cell proliferation, angiogenesis, metastasis as well as immune escape. In this review, we summarize the current knowledge about exosomal circRNAs in cancers and discuss their specific functions in tumorigenesis. Additionally, we discuss the potential value of exosomal circRNAs as diagnostic biomarkers and the potential applications of exosomal circRNA-based cancer therapy.
A novel explainable online calculator for contrast-induced AKI in diabetics: a multi-centre validation and prospective evaluation study
Authors: Ma Mengqing , Wan Xin , Chen Yuyang , Lu Zhichao , Guo Danning , Kong Huiping , Pan Binbin , Zhang Hao , Chen Dawei , Xu Dongxu , Sun Dong , Lang Hong , Zhou Changgao , Li Tao , Cao Changchun
Published: July 2023

Background

In patients undergoing percutaneous coronary intervention (PCI), contrast-induced acute kidney injury (CIAKI) is a frequent complication, especially in diabetics, and is connected with severe mortality and morbidity in the short and long term. Therefore, we aimed to develop a CIAKI predictive model for diabetic patients.

Methods

3514 patients with diabetes from four hospitals were separated into three cohorts: training, internal validation, and external validation. We developed six machine learning (ML) algorithms models: random forest (RF), gradient-boosted decision trees (GBDT), logistic regression (LR), least absolute shrinkage and selection operator with LR, extreme gradient boosting trees (XGBT), and support vector machine (SVM). The area under the receiver operating characteristic curve (AUC) of ML models was compared to the prior score model, and developed a brief CIAKI prediction model for diabetes (BCPMD). We also validated BCPMD model on the prospective cohort of 172 patients from one of the hospitals. To explain the prediction model, the shapley additive explanations (SHAP) approach was used.

Results

In the six ML models, XGBT performed best in the cohort of internal (AUC: 0.816 (95% CI 0.777–0.853)) and external validation (AUC: 0.816 (95% CI 0.770–0.861)), and we determined the top 15 important predictors in XGBT model as BCPMD model variables. The features of BCPMD included acute coronary syndromes (ACS), urine protein level, diuretics, left ventricular ejection fraction (LVEF) (%), hemoglobin (g/L), congestive heart failure (CHF), stable Angina, uric acid (umol/L), preoperative diastolic blood pressure (DBP) (mmHg), contrast volumes (mL), albumin (g/L), baseline creatinine (umol/L), vessels of coronary artery disease, glucose (mmol/L) and diabetes history (yrs). Then, we validated BCPMD in the cohort of internal validation (AUC: 0.819 (95% CI 0.783–0.855)), the cohort of external validation (AUC: 0.805 (95% CI 0.755–0.850)) and the cohort of prospective validation (AUC: 0.801 (95% CI 0.688–0.887)). SHAP was constructed to provide personalized interpretation for each patient. Our model also has been developed into an online web risk calculator. MissForest was used to handle the missing values of the calculator.

Conclusion

We developed a novel risk calculator for CIAKI in diabetes based on the ML model, which can help clinicians achieve real-time prediction and explainable clinical decisions.
Reduced bronchoalveolar macrophage phagocytosis and cytotoxic effects after controlled short-term exposure to wood smoke in healthy humans
Authors: Hansson Alva , Rankin Gregory , Uski Oskari , Friberg Maria , Pourazar Jamshid , Lindgren Robert , García-López Natxo , Boman Christoffer , Sandström Thomas , Behndig Annelie , Muala Ala
Published: July 2023

Background

Exposure to wood smoke has been shown to contribute to adverse respiratory health effects including airway infections, but the underlying mechanisms are unclear. A preceding study failed to confirm any acute inflammation or cell influx in bronchial wash (BW) or bronchoalveolar lavage (BAL) 24 h after wood smoke exposure but showed unexpected reductions in leukocyte numbers. The present study was performed to investigate responses at an earlier phase, regarding potential development of acute inflammation, as well as indications of cytotoxicity.

Methods

In a double-blind, randomised crossover study, 14 healthy participants were exposed for 2 h to filtered air and diluted wood smoke from incomplete wood log combustion in a common wood stove with a mean particulate matter concentration of 409 µg/m3. Bronchoscopy with BW and BAL was performed 6 h after exposure. Differential cell counts, assessment of DNA-damage and ex vivo analysis of phagocytic function of phagocytosing BAL cells were performed. Wood smoke particles were also collected for in vitro toxicological analyses using bronchial epithelial cells (BEAS-2B) and alveolar type II-like cells (A549).

Results

Exposure to wood smoke increased BAL lactate dehydrogenase (LDH) (p = 0.04) and reduced the ex vivo alveolar macrophage phagocytic capacity (p = 0.03) and viability (p = 0.02) vs. filtered air. BAL eosinophil numbers were increased after wood smoke (p = 0.02), while other cell types were unaffected in BW and BAL. In vitro exposure to wood smoke particles confirmed increased DNA-damage, decreased metabolic activity and cell cycle disturbances.

Conclusions

Exposure to wood smoke from incomplete combustion did not induce any acute airway inflammatory cell influx at 6 h, apart from eosinophils. However, there were indications of a cytotoxic reaction with increased LDH, reduced cell viability and impaired alveolar macrophage phagocytic capacity. These findings are in accordance with earlier bronchoscopy findings at 24 h and may provide evidence for the increased susceptibility to infections by biomass smoke exposure, reported in population-based studies.
COVID-19 crisis interlinkage with past pandemics and their effects on food security
Authors: Roubík Hynek , Lošťák Michal , Ketuama Chama Theodore , Soukupová Jana , Procházka Petr , Hruška Adam , Hakl Josef , Pacek Lukáš , Karlík Petr , Menšíková Lucie Kocmánková , Jurasová Vladimíra , Ogbu Charles Amarachi , Hejcman Michal
Published: July 2023

Background

Pandemics as health and humanitarian crises have exerted traceable impacts on food security. Almost all past and current pandemics have created a food crisis that affects a share of the global population and threaten global food security. With the more frequent outbreaks of emerging and re-emerging diseases or pandemics, this paper looks at the various types of impacts from the current coronavirus crisis and past pandemics to identify their major impact on food security.

Scope

To this effect, key strategies that could be put in place to ensure the efficient resilience of food systems before, during, and after the pandemics to mitigate the negative impact of the pandemics on global food security are recommended. The most recent effects of the current coronavirus crisis have been disruptions in the flow of farm labourers and inefficient farm operations leading to postharvest food losses.

Key findings and conclusions

Modification of diets between social groups has also been observed. Future response orientations to prevent and mitigate the effects of pandemics on food security will consider pro-active and adapted policy, program, and institutional actions towards the systemic development of global food systems as an interconnected network.
A meta-analysis of immune-cell fractions at high resolution reveals novel associations with common phenotypes and health outcomes
Authors: Luo Qi , Dwaraka Varun B. , Chen Qingwen , Tong Huige , Zhu Tianyu , Seale Kirsten , Raffaele Joseph M. , Zheng Shijie C. , Mendez Tavis L. , Chen Yulu , Carreras Natalia , Begum Sofina , Mendez Kevin , Voisin Sarah , Eynon Nir , Lasky-Su Jessica A. , Smith Ryan , Teschendorff Andrew E.
Published: July 2023

Background

Changes in cell-type composition of tissues are associated with a wide range of diseases and environmental risk factors and may be causally implicated in disease development and progression. However, these shifts in cell-type fractions are often of a low magnitude, or involve similar cell subtypes, making their reliable identification challenging. DNA methylation profiling in a tissue like blood is a promising approach to discover shifts in cell-type abundance, yet studies have only been performed at a relatively low cellular resolution and in isolation, limiting their power to detect shifts in tissue composition.

Methods

Here we derive a DNA methylation reference matrix for 12 immune-cell types in human blood and extensively validate it with flow-cytometric count data and in whole-genome bisulfite sequencing data of sorted cells. Using this reference matrix, we perform a directional Stouffer and fixed effects meta-analysis comprising 23,053 blood samples from 22 different cohorts, to comprehensively map associations between the 12 immune-cell fractions and common phenotypes. In a separate cohort of 4386 blood samples, we assess associations between immune-cell fractions and health outcomes.

Results

Our meta-analysis reveals many associations of cell-type fractions with age, sex, smoking and obesity, many of which we validate with single-cell RNA sequencing. We discover that naïve and regulatory T-cell subsets are higher in women compared to men, while the reverse is true for monocyte, natural killer, basophil, and eosinophil fractions. Decreased natural killer counts associated with smoking, obesity, and stress levels, while an increased count correlates with exercise and sleep. Analysis of health outcomes revealed that increased naïve CD4 + T-cell and N-cell fractions associated with a reduced risk of all-cause mortality independently of all major epidemiological risk factors and baseline co-morbidity. A machine learning predictor built only with immune-cell fractions achieved a C-index value for all-cause mortality of 0.69 (95%CI 0.67–0.72), which increased to 0.83 (0.80–0.86) upon inclusion of epidemiological risk factors and baseline co-morbidity.

Conclusions

This work contributes an extensively validated high-resolution DNAm reference matrix for blood, which is made freely available, and uses it to generate a comprehensive map of associations between immune-cell fractions and common phenotypes, including health outcomes.
Targeted screening and identification of chlorhexidine as a pro-myogenic circadian clock activator
Authors: Kiperman Tali , Li Weini , Xiong Xuekai , Li Hongzhi , Horne David , Ma Ke
Published: July 2023

Background

The circadian clock is an evolutionarily conserved mechanism that exerts pervasive temporal control in stem cell behavior. This time-keeping machinery is required for orchestrating myogenic progenitor properties in regenerative myogenesis that ameliorates muscular dystrophy. Here we report a screening platform to discover circadian clock modulators that promote myogenesis and identify chlorhexidine (CHX) as a clock-activating molecule with pro-myogenic activities.

Methods

A high-throughput molecular docking pipeline was applied to identify compounds with a structural fit for a hydrophobic pocket within the key circadian transcription factor protein, Circadian Locomotor Output Cycles Kaput (CLOCK). These identified molecules were further screened for clock-modulatory activities and functional validations for pro-myogenic properties.

Results

CHX was identified as a clock activator that promotes distinct aspects of myogenesis. CHX activated circadian clock that reduced cycling period length and augmented amplitude. This action was mediated by the targeted CLOCK structure via augmented interaction with heterodimer partner Bmal1, leading to enhanced CLOCK/Bmal1-controlled transcription with upregulation of core clock genes. Consistent with its clock-activating function, CHX displayed robust effects on stimulating myogenic differentiation in a clock-dependent manner. In addition, CHX augmented the proliferative and migratory activities of myoblasts.

Conclusion

Our findings demonstrate the feasibility of a screening platform to discover clock modulators with myogenic regulatory activities. Discovery of CHX as a pro-myogenic molecule could be applicable to promote regenerative capacities in ameliorating dystrophic or degenerative muscle diseases.
Differential Salmonella Typhimurium intracellular replication and host cell responses in caecal and ileal organoids derived from chicken
Authors: Lacroix-Lamandé Sonia , Bernardi Ophélie , Pezier Tiffany , Barilleau Emilie , Burlaud-Gaillard Julien , Gagneux Anissa , Velge Philippe , Wiedemann Agnès
Published: July 2023

Chicken infection with Salmonella Typhimurium is an important source of foodborne human diseases. Salmonella colonizes the avian intestinal tract and more particularly the caecum, without causing symptoms. This thus poses a challenge for the prevention of foodborne transmission. Until now, studies on the interaction of Salmonella with the avian gut intestine have been limited by the absence of in vitro intestinal culture models. Here, we established intestinal crypt‐derived chicken organoids to better decipher the impact of Salmonella intracellular replication on avian intestinal epithelium. Using a 3D organoid model, we observed a significantly higher replication rate of the intracellular bacteria in caecal organoids than in ileal organoids. Our model thus recreates intracellular environment, allowing Salmonella replication of avian epithelium according to the intestinal segment. Moreover, an inhibition of the cellular proliferation was observed in infected ileal and caecal organoids compared to uninfected organoids. This appears with a higher effect in ileal organoids, as well as a higher cytokine and signaling molecule response in infected ileal organoids at 3 h post-infection (hpi) than in caecal organoids that could explain the lower replication rate of Salmonella observed later at 24 hpi. To conclude, this study demonstrates that the 3D organoid is a model allowing to decipher the intracellular impact of Salmonella on the intestinal epithelium cell response and illustrates the importance of the gut segment used to purify stem cells and derive organoids to specifically study epithelial cell -Salmonella interaction.
Decomposition and nutrient dynamics of stumps and coarse roots of Eucalyptus plantations in southern China
Authors: Deng Xiangsheng , Liang Xiang , Shen Lu , Liu Haiyu , Yang Mei , Zeng Min , Liang Mengqiang , Cheng Fei
Published: July 2023

Key message

Primary remains of Eucalyptus (E. urophylla × E. grandis) plantations following clear-cutting are the stumps and coarse roots. A chrono-sequence approach revealed that the biomass of coarse roots is higher than that of stumps, while they have a smaller rate of biomass loss than stumps. This implies that coarse roots serve as long-term stock for nutrients (carbon, nitrogen, and phosphorus).

Context

A significant quantity of stumps and coarse roots persist in the forest floor following the clear-cutting of Eucalyptus plantations in China. The decomposition of these stumps and coarse roots is a crucial aspect of the nutrient stocks in plantation ecosystems.

Aims

We described the stock and decomposition dynamics of stumps and coarse roots on Eucalyptus plantations, as well as the nutrient loss rate associated with them.

Methods

We used a chrono-sequence of 0–6-year-old clear-cutting stands of Eucalyptus plantations in southern China. The biomass and nutrient stocks of these samples were assessed at different times of decomposition.

Results

The highest biomass of stump-root system was 33.8 Mg·ha−1, with nutrient stocks were 16.3 Mg·ha−1, and 63.0 kg·ha−1, and 6710.9 g·ha−1, for C, N, and P. The coarse roots accounted for 81–96% of the biomass of the stump-root system. The loss rate of biomass and nutrients from stumps was higher than that of coarse roots. This suggests that the decomposition of coarse roots could serve as a long-term source of nutrients, thereby improving the nutrient status of the plantations.

Conclusion

Stumps and coarse roots serve as significant nutrient stocks that decay at varying rates. The loss of nutrients must be considered while analyzing the decomposition dynamics following clear-cutting in Eucalyptus plantations.
Aso volcano, Japan: assessing the 100-year probability of a new caldera-forming eruption based on expert judgements with Bayes Net and Importance Sampling uncertainty analysis
Authors: Aspinall Willy , Sparks R. Stephen J. , Hill Brittain E. , Costa Antonio , Connor Charles , Inakura Hirohito , Hasenaka Toshiaki , Miyoshi Masaya , Kiyosugi Koji , Tsuji Tomohiro , Ushioda Masashi
Published: July 2023

The Aso-4 explosive eruption on Kyushu, Japan, 89,500 years ago was one of the biggest eruptions in the last one hundred millennia, with a magnitude of approximately M8. Modern society requires the likelihood of natural events with potentially disastrous consequences to be evaluated, even if probabilities of occurrence are diminishingly small. For some situations, it is not satisfactory to assert an event scenario probability is “negligible” or can be “ignored”. Judicial hearings or litigation may require risk levels to be quantified, in which case, statements of scientific confidence could be decisive. Internationally, e.g., for nuclear site safety evaluations, event likelihoods on order of 10–7/year are often considered for quantitative assessment. At such hazard levels, this might include evaluating the proposition that a particular volcano can deliver a future super-eruption, a supposition that could be attached to Aso volcano. But, simplistically taking the average recurrence interval between past caldera-forming eruptions at a given volcano is an unreliable guide to the likelihood of a future repeat: each past event represented a unique set of tectonic and magmatic conditions within a continually evolving volcanic system. Such processes are not temporally stationary nor statistically uniform. To evaluate the probability of a new M8 event at Aso, within the next 100 years, we performed a comprehensive stochastic probability uncertainty analysis using a model implemented with advanced computational Bayes Net (BN) software. Our eruption process model is informed by multiple strands of evidence from volcanology, petrology, geochemistry and geophysics, together with estimates of epistemic (knowledge) uncertainty, adduced from reviews of published data, modelling and from expert judgement elicitation. Several lines of evidence characterise the likely structure, magmatic composition and eruptive state of the present-day Aso volcano, which has had numerous smaller eruptions since Aso-4. To calculate the probability of another M8 eruption of Aso, we implemented probabilistic ‘Importance Sampling’ in our model. With this approach, we find the chance of an Aso-4 scale eruption (characterised by mean volume 500 km3DRE and approximate 90% credible interval [210 ‥ 1200] km3DRE) is less than 1–in–1 billion in the next 100 years (i.e., < 10–9probability). Based on current volcanological understanding and evidence, we believe this probability estimate is robust to within an order of magnitude.
Horizon scanning: what next for bovine TB control in England?
Authors: McCormack James
Published: July 2023

England is currently in year nine of its 25-year strategy to achieve TB freedom. This talk will speculate on what new tools and approaches could be introduced in the future to help us achieve our goal.

Using Defra’s response to the independent review of its TB programme as a starting point and building on the plenary talk by the UK CVO, I will look at some opportunities that could arise under the different aspects of the response.

Firstly, how best to help farmers reduce their herd TB risk through better informed purchasing decisions will be considered, including looking at the recent publication of the health ratings for every cattle herd in England.

Cattle vaccination, and its associated DIVA test could be the biggest change in Tb control in England in many years while the related development of a molecularly defined tuberculin which could become the default testing reagent.

Advances in whole genome sequencing will allow us to sequence the genome of M.bovis isolated from most infected herds in England and these data could unlock a variety of opportunities from tracing the spread of infection to ground-truthing the efficacy of testing and epidemiological assessment of breakdowns.

Finally, the move to vaccination as the primary way of controlling TB in badgers with culling used very sparingly will be considered using a case study of how a targeted badger cull successfully removed infection from an area in Cumbria and enabled the switch to vaccination.
Exploring the contribution of case study research to the evidence base for occupational therapy: a scoping review
Authors: McQuaid Leona , Thomson Katie , Bannigan Katrina
Published: July 2023

Background

Case study research is generating interest to evaluate complex interventions. However, it is not clear how this is being utilized by occupational therapists or how feasible it is to contribute to the evidence base. This scoping review explores case study research within occupational therapy in terms of how it is defined, the methodological characteristics adopted, such as data collection and analysis, and the range of practice contexts in which it is applied. We consider the viability of case study research for contributing to our evidence base.

Methods

Opinion, text and empirical studies within an occupational therapy practice context were included. A three-step extensive search following Joanna Briggs Institute methodology was conducted in June 2020 and updated in July 2021 across ten databases, websites, peer-reviewed and grey literature from 2016 onwards. Study selection was completed by two independent reviewers. A data extraction table was developed and piloted and data charted to align with research questions. Data extraction was completed by one reviewer and a 10% sample cross checked by another.

Results

Eighty-eight studies were included in the review consisting of (n = 84) empirical case study and (n = 4) non-empirical papers. Case study research has been conducted globally, with a range of populations across different settings. The majority were conducted in a community setting (n= 48/84; 57%) with populations experiencing neurodevelopmental disorder (n= 32/84; 38%), stroke (n= 14/84;17%) and non-diagnosis specific (n= 13/84; 15%). Methodologies adopted quantitative (n= 42/84; 50%), mixed methods (n= 22/84; 26%) and qualitative designs (n= 20/84; 24%). However, identifying the methodology and ‘case’ was a challenge due to methodological inconsistencies.

Conclusions

Case study research is useful when large-scale inquiry is not appropriate; for cases of complexity, early intervention efficacy, theory testing or when small participant numbers are available. It appears a viable methodology to contribute to the evidence base for occupation and health as it has been used to evaluate interventions across a breadth of occupational therapy practice contexts. Viability could be enhanced through consistent conduct and reporting to allow pooling of case data. A conceptual model and description of case study research in occupational therapy is proposed to support this.

Systematic review registration

Open Science Framework 10.17605/OSF.IO/PCFJ6.
Inhibitory control towards angry stimuli in patients with binge eating disorder: a pilot study
Authors: Schag Kathrin , Sandler Lea , Zipfel Stephan , Derntl Birgit , Giel Katrin Elisabeth
Published: July 2023

Background

Emotion regulation theories and the negative urgency concept assume that negative mood increases binge eating. Negative emotions are considered as a trigger for binge eating, while binge eating itself is regarded as an impulsive behavior and should thus be increased within the negative urgency concept. Anger might be a specific negative emotion triggering binge eating in patients with binge eating disorder (BED). We investigated how inhibitory control as one main factor of impulsivity is influenced by anger stimuli in patients with BED and two control groups.

Methods

We compared patients with BED (N = 20) with normal-weight healthy control participants (NW-CG, N = 20) and BMI-matched overweight and obese control participants (BMI-CG, N = 18). We used the emotional Stop Signal task (eSST) to investigate inhibitory control, where we presented angry facial expressions in comparison with neutral facial expressions as emotional stimuli.

Results

All participants showed decreased inhibitory control in the angry versus neutral condition, i.e., a faster Stop Signal Reaction Time and a lower percentage of correct reactions. However, no significant group differences emerged in terms of performance. Performance in the eSST did not correlate with negative urgency, disorder- or emotion-related characteristics.

Conclusions

The current pilot study does not deliver evidence for decreased inhibitory control towards angry stimuli in patients with BED, as we detected a general and not disorder-related effect in all participants that might represent the conjunction of inhibitory control and anger. A direct mood induction technique might have led to different results. Further research in healthy and clinical groups is needed.
Children’s injury database: development of an injury surveillance system in a pediatric emergency department
Authors: McCain Jennifer E. , Bridgmon Ashley E. , King William D. , Monroe Kathy
Published: July 2023

Background

Injuries are the leading cause of death in children and are also a leading cause of all emergency department (ED) visits for children. Obtaining epidemiologic data to define the wide range of childhood injuries for individual communities is challenging. The Children’s Injury Database (CID) is an injury surveillance system developed to collect data from injury-related visits to our tertiary care pediatric emergency department.

Results

During 2021, a total of 15,168 injury visits were analyzed representing 22% of total ED visits (68,834). A total of 2053 injury visits (13.5%) resulted in hospital admission. The 10 leading injury types included: falls, poisonings, motor vehicle collision (MVC), assault, dog bite, burns, sports, pedestrian, bicycle, and all-terrain vehicle (ATV). Admission rates varied by age group with children ages 13 years and older having the highest rate of admission (18.4%). The median length of stay (LOS) for all injured children requiring admission was 2 days while the median LOS for preschoolers was 1 day, the median LOS for school-age children was 2 days, and the median LOS for teenagers was 3 days. While MVCs were the most common cause of vehicle-related injuries, ATV-related injuries had the highest rate of admission (51%).

Conclusions

In this study, teenagers had significantly higher admission rates, lengths of stay, and hospital charges. Black and Hispanic children were under-represented in the number of visits for injuries compared to all ED visits. Further research should focus on disparities in injury-related visits based on race as well as gender. CID has demonstrated that injury surveillance systems can assist with reporting new injury patterns while also acting as a stimulus for new research ideas, planning interventions targeting the most at-risk populations, and evaluating the effectiveness of injury prevention interventions.
Robotic‐assisted technology does not influence functional outcomes among obese and morbidly obese total knee arthroplasty patients
Authors: McCormick Brian P. , Trent Sarah , Geng Xue , Lee Ji Won , Boucher Henry R.
Published: July 2023

Purpose

Despite benefits of total knee arthroplasty (TKA) on function and quality of life, obese patients have less improved functional outcomes following TKA compared to their normal weight counterparts. Furthermore, obesity is a risk factor for aseptic loosening and revision surgery following TKA. With known benefits of robotic‐assisted TKA (RaTKA) in precision and patient satisfaction, we aimed to evaluate the differences in patient reported outcome and early complication rates for patients undergoing RaTKA versus conventional TKA among patients of varying BMI groups.

Methods

This study was a retrospective cohort study of patients who underwent conventional versus RaTKA. Patients were grouped by BMI range (< 30 kg/m2, 30–40 kg/m2, and > 40 kg/m2). Patient‐reported outcomes were measured by Oxford Knee Scores and 12‐Item Short Form Survey scores preoperatively, 6‐month, 1‐year, and 2‐year postoperatively. Mixed‐effects linear models were built for each patient‐reported outcome to assess the interaction between type of surgery and BMI while adjusting for known confounders such as demographic variables.

Results

A total of 350 patients (n = 186 RaTKA, n = 164 conventional TKA) met inclusion criteria. SF‐12 physical scores were significantly higher at 2‐year follow‐up among non‐obese patients compared to obese and morbidly obese patients (p = 0.047). There was no statistically significant interaction between the type of surgery performed (RaTKA versus conventional TKA) and obesity regarding their effects on patient reported outcomes.

Conclusions

This study demonstrates no differences in functional outcomes among patients undergoing RaTKA compared to conventional TKA. Furthermore, obesity had no significant effect on this association.

Level of evidence

III.
Progress in modeling the Tohoku-oki megathrust earthquake cycle and associated crustal deformation processes
Authors: Shibazaki Bunichiro
Published: July 2023

This paper summarizes the results of 10 years of research on models of the megathrust earthquake cycles and crustal deformation associated with the 2011 Tohoku-oki earthquake. Several earthquake cycle models have been proposed for the northeast Japan subduction zone to elucidate why megathrust earthquakes occur at intervals of approximately 600 years and why large slips occurred in the shallow subduction zone. A model that considers a strong asperity in the shallow plate interface, and a hierarchical asperity model that considers the scale dependence of the critical displacement of the rate- and state-dependent friction law have been proposed. Modeling with dynamic weakening of faults has also been proposed. In the model using the shallow friction characteristics obtained by the Japan Trench Fast Drilling Project, rupture from depth can propagate to the trench, resulting in shallow large slips. Submarine crustal deformation has been observed for the first time in addition to dense observations of the inland crustal deformation. The observation of the seafloor deformation near the trench showed that viscoelastic relaxation played an important role in short-term postseismic deformation near the trench. The effects of the low-viscosity region at the oceanic lithosphere and asthenosphere boundary, and the cold forearc mantle wedge (cold nose) have been discussed. Simulations using the nonlinear flow law of rock in the mantle, where a power–law relationship holds between stress and strain rate, and the fault friction law at the plate boundary, show that the Tohoku-oki earthquake caused large stress fluctuations, resulting in a sudden viscosity decrease and rapid flow in the asthenosphere below the oceanic lithosphere. The simulations of the crustal deformation associated with the Tohoku-oki earthquake cycle also indicate that in the later stage of the earthquake cycle, the Pacific coastal region begins to subside due to the increasing slip deficit rate on the deeper parts of the plate interface. These results explain the subsidence of the Pacific coast of northeast Japan observed for about 100 years prior to the Tohoku-oki earthquake. In the future, a model that explains the long-term crust and mantle deformation during the entire Tohoku-oki earthquake cycle must be constructed.