bluuebunny/crossref_metadata_2025 huggingface dataset:
Healthcare professionals and scientists’ collaboration with biobanks: a pilot study on the assessment of knowledge and attitudes toward biospecimen donation
Authors: Mikhailova Anastasiia A. , Bogomiagkova Elena S. , Nasykhova Yulia A. , Illarionov Roman A. , Danilova Maria M. , Tonyan Ziravard N. , Chernykh Vyacheslav B. , Kovalenko Ludmila V. , Bespalova Olesya N. , Glotov Andrey S.
Published: February 2025

Background

Difficulties in the biobank progress are often associated with insufficient public awareness, but it is also worth paying attention to healthcare and science professionals who play an important role in the development of this area. This article presents results of the research on awareness toward biobanking and motivation to collaborate among employees of 3 medical and scientific organizations in Russia.

Methods

The anonymous survey was carried out via an online platform. The research included 176 healthcare and science professionals. To assess the differences between the groups the Pearson Chi-square test and the contingency coefficient were used. To find correlations between nominal and interval variables the Eta criterion was applied.

Results

It was found that 88.1% of respondents were aware of biobanking in general, however, 15.0% were not aware of a biobank in their organization or had difficulty in answering this question. The experience of cooperation with biobanks was not particularly extensive – more than half of the respondents (60.3%) indicated that they had never contacted biobanks. 11.9% of participants became donors themselves. 35.0% - suggested to their friends and patients to become donors, while 86.2% were willing to do this in the future. The study showed that the respondents who already had experience working with biobanks, as well as those who themselves act as a donor, rated the importance of their activities higher.

Conclusion

It is important to increase not only awareness but also motivation to cooperate with biobanks and be willing to take on different roles.
Bibliometric analysis of preoperative radiotherapy for locally advanced rectal cancer: evolution and future
Authors: Weng Zhen-Hong , Hu Hao-Kai , Zhou Zi-Shan , Huang Li-Sheng , Chen Bin-Bin , Lin Jia-Rui
Published: February 2025

Background

Preoperative radiotherapy of rectal cancer has been a hot topic of research in recent years with the introduction of total neoadjuvant therapies and immunotherapeutic agents. We utilized bibliometrics and visualization analysis to examine studies in this field, aiming to identify current hotspots and research trends.

Method

We searched the Web of Science database for all publications related to preoperative radiotherapy of rectal cancer in the past 10 years. Using bibliometric analysis software, such as VOSviewer, CiteSpace and R-studio, we extracted and analyzed the data, summarizing the publication output of countries, institutions, authors, and journals in this field, and analyzing their relationships. We also summarized the keywords, burst words, and most cited articles, and analyzed the relationships among them.

Results

We found 794 publications in the field, sourced from 217 journals or books, involving 5,805 authors from various organizations and countries. Through bibliometric analysis, we observed a growing trend in the number of publications in preoperative radiotherapy of rectal cancer over the past 10 years. China, United States and Italy were the top countries in terms of publication output. Sun Yat-sen University, Fujian University, and Fudan University were the top three medical centers in terms of publication output, while Leiden University from Netherlands led globally in terms of citation impact. Professor Zhen Zhang, Sanjun Cai, and Ji Zhu were the top three authors with the highest publication output. The most highly cited journals in this field includes “The Lancet Oncology,” “J Clinical Oncology,” and “Annals of Oncology.” Journals such as “Radiotherapy and Oncology,” “Frontiers in Oncology,” and “BMC Cancer” have the highest number of articles published. Based on the analysis of keywords and burst words, we found that “preoperative chemoradiation” and “oral capecitabine” were the research hotspots before 2016, while the focus shifted to “short-course radiotherapy” and “long-term outcomes” after 2017. Currently, the most frequently cited publications mainly summarize multicenter clinical studies and total neoadjuvant treatment models and immunotherapy.

Conclusion

Research on preoperative radiotherapy of rectal cancer is increasing year by year, and attracting attention from high-cited journals such as “The Lancet Oncology,” “JCO,” and “Annals of Oncology.” Based on current data, the total neoadjuvant treatment models and radiation combined with immunotherapy are the research trends.
Genome-wide CRISPR/Cas9 screening identifies key profibrotic regulators of TGF-β1-induced epithelial-mesenchymal transformation and pulmonary fibrosis
Authors: Tan Chunjiang , Wang Juan , Ye Xiangrong , Kasimu Kaidirina , Li Ye , Luo Feng , Yi Hui , Luo Yifeng
Published: February 2025

Background

The idiopathic pulmonary fibrosis (IPF) is a progressive and lethal interstitial lung disease with high morbidity and mortality. IPF is characterized by excessive extracellular matrix accumulation (ECM) and epithelial-mesenchymal transformation (EMT). To date, few anti-fibrotic therapeutics are available to reverse the progression of pulmonary fibrosis, and it is important to explore new profibrotic molecular regulators mediating EMT and pulmonary fibrosis.

Methods

Based on our model of TGF-β1-induced EMT in BEAS-2B cells, we performed the genome-wide CRISPR/Cas9 knockout (GeCKO) screening technique, pathway and functional enrichment analysis, loss-of-function experiment, as well as other experimental techniques to comprehensively investigate profibrotic regulators contributing to EMT and the pathogenesis of pulmonary fibrosis.

Results

Utilizing the GeCKO library screening, we identified 76 top molecular regulators. Ten candidate genes were subsequently confirmed by integrating the high-throughput data with findings from pathway and functional enrichment analysis. Among the candidate genes, knockout of COL20A1 and COL27A1 led to decreased mRNA expression of ECM components (Fibronectin and Collagen-I), as well as an increased rate of cell apoptosis. The mRNA expression of Collagen-I, together with the cell viability and migration, were inhibited when knocking out the WNT11. In addition, a decrease in the protein deposition of ECM components was observed by suppressing the expression of COL20A1, COL27A1, and WNT11.

Conclusion

Our study demonstrates that the COL20A1, COL27A1, and WNT11 serve as key profibrotic regulators of EMT. Gaining understanding and insights into these key profibrotic regulators of EMT paves the way for the discovery of new therapeutic targets against the onset and progression of IPF.
Electroencephalographic differences between waking and sleeping periods in patients with prolonged disorders of consciousness at different levels of consciousness
Authors: Li Keke , Li Man , Liu Wanqing , Wu Yanzhi , Li Fang , Xie Jingwei , Zhou Shaolong , Wang Sen , Guo Yongkun , Pan Jiahui , Wang Xinjun
Published: February 2025

Objective

This study aimed to explore differences in sleep electroencephalogram (EEG) patterns in individuals with prolonged disorders of consciousness, utilizing polysomnography (PSG) to assist in distinguishing between the vegetative state (VS)/unresponsive wakefulness syndrome (UWS) and the minimally conscious state (MCS), thereby reducing misdiagnosis rates and enhancing the quality of medical treatment.

Methods

A total of 40 patients with prolonged disorders of consciousness (pDOC; 27 patients in the VS/UWS and 13 in the MCS) underwent polysomnography. We analyzed differential EEG indices between VS/UWS and MCS groups and performed correlation analyses between these indices and the Coma Recovery Scale-Revised (CRS-R) scores. The diagnostic accuracy of the differential indices was evaluated using receiver operating characteristic (ROC) curves.

Results

1. The fractal dimension (Higuchi’s fractal dimension (HFD)) of patients in the MCS tended to be higher than that of patients in the VS/UWS across all phases, with a significant difference only in the waking phase (p < 0.05). The HFD in the waking phase was positively correlated with the CRS-R score and exhibited the highest diagnostic accuracy at 88.3%. The Teager–Kaiser energy operator (TKEO) also showed higher levels in patients in the MCS compared to those in the VS/UWS, significantly so in the NREM2 phase (p < 0.05), with a positive correlation with the CRS-R score and diagnostic accuracy of 75.2%. The δ-band power spectral density [PSD(δ)] in the patients in the MCS was lower than that in those in the VS/UWS, significantly so in the waking phase (p < 0.05), and it was negatively correlated with the CRS-R score, with diagnostic accuracy of 71.5%.

Conclusion

Polysomnography for the VS/UWS and MCS revealed significant differences, aiding in distinguishing between the two patient categories and reducing misdiagnosis rates. Notably, the HFD and PSD(δ) showed significantly better performance during wakefulness compared to sleep, while the TKEO was more prominent in the NREM2 stage. Notably, the HFD exhibited a robust correlation with the CRS-R scores, the highest diagnostic accuracy, and immense promise in the clinical diagnosis of prolonged disorders of consciousness.
Case report: Re-evaluating reversibility of cytotoxic lesions of the corpus callosum
Authors: Vold Victoria , Tingvoll Stein-Helge Hansen , Beyer Mona K. , Nordengen Kaja
Published: February 2025

Cytotoxic lesions of the corpus callosum (CLOCC) are a clinicoradiological diagnosis, characterized by transient neurological symptoms and magnetic resonance imaging (MRI) changes in the splenium of the corpus callosum (SCC), which in most cases is completely reversible. However, the long-term pathophysiological trajectory and ultimate neurological outcomes of CLOCC remain largely unknown due to limited long-term follow-up data. We report an 11-year follow-up of a postpartum female with CLOCC, initially presenting with transient focal neurological symptoms and extensive diffusion-restricted white matter involvement including the SCC and surrounding area with diffusion restriction and low apparent diffusion coefficient values, indicative of cytotoxic edema. The edema regressed in days; over the years, she remained asymptomatic despite persistent white matter changes on MRI in the centrum semiovale. This case challenges the view of CLOCC as completely reversible and raises questions regarding the significance of lasting white matter changes. The enduring absence of neurological symptoms and stable radiological profile throughout the decade underscores the singular nature of CLOCC and the lasting, though isolated, impact on white matter. This report contributes a crucial perspective, suggesting that CLOCC may involve just an isolated episode without recurrent events or progressive neurological decline. By offering the first longitudinal analysis of a CLOCC episode with an extended follow-up of over a decade, our case enhances current knowledge about the long-term neurological and radiological landscape of this condition. It suggests a reevaluation of the conceptual understanding of CLOCC as an entirely reversible, non-relapsing disorder, highlighting the need for further research into its long-term impacts on cerebral white matter integrity.
Postsynaptic spiking determines anti-Hebbian LTD in visual cortex basket cells
Authors: Chou Christina Y. C. , Droogers Wouter J. , Lalanne Txomin , Fineberg Eric , Klimenko Tal , Owens Hannah , Sjöström P. Jesper
Published: February 2025

Long-term plasticity at pyramidal cell to basket cell (PC → BC) synapses is important for the functioning of cortical microcircuits. It is well known that at neocortical PC → PC synapses, dendritic calcium (Ca2+) dynamics signal coincident pre-and postsynaptic spiking which in turn triggers long-term potentiation (LTP). However, the link between dendritic Ca2+ dynamics and long-term plasticity at PC → BC synapses of primary visual cortex (V1) is not as well known. Here, we explored if PC → BC synaptic plasticity in developing V1 is sensitive to postsynaptic spiking. Two-photon (2P) Ca2+ imaging revealed that action potentials (APs) in dendrites of V1 layer-5 (L5) BCs back-propagated decrementally but actively to the location of PC → BC putative synaptic contacts. Pairing excitatory inputs with postsynaptic APs elicited dendritic Ca2+ supralinearities for pre-before-postsynaptic but not post-before-presynaptic temporal ordering, suggesting that APs could impact synaptic plasticity. In agreement, extracellular stimulation as well as high-throughput 2P optogenetic mapping of plasticity both revealed that pre-before-postsynaptic but not post-before-presynaptic pairing resulted in anti-Hebbian long-term depression (LTD). Our results demonstrate that V1 BC dendritic Ca2+ nonlinearities and synaptic plasticity at PC → BC connections are both sensitive to somatic spiking.
Ketogenic diet and microRNAs: focus on cognitive function
Authors: Abrego-Guandique Diana Marisol , Cione Erika , Caroleo Maria Cristina , Bonilla Diego A. , Cannataro Roberto
Published: February 2025

Cognition is a mental process of understanding and learning driven by memory. Recent advances in molecular biology and neuroscience have revealed a fascinating interplay between cognitive function and microRNAs (miRNAs). The ketogenic diet (KD) is a low-carbohydrate, high-fat, and adequate-protein diet that triggers the synthesis of ketone bodies, establishing ketosis. Recent and accumulating studies on human and animal models have shown that the KD benefits neurodegenerative diseases, where cognition is affected. The KD can also modulate miRNAs, molecules that are dysregulated in the brains of individuals with Alzheimer’s disease, where cognition is lost. In this mini-review, we provide an overview of the function of miRNAs in neurodevelopment and cognition. We also explore how the KD in human studies can enhance cognitive function and highlight the protective role of microRNAs in neurological conditions.
Immunoregulatory role of exosomal circRNAs in the tumor microenvironment
Authors: Lv Cunming , Chen Jinhao , Wang Yuxiang , Lin Yichen
Published: February 2025

As cancer incidence and mortality rates rise, there is an urgent need to develop effective immunotherapy strategies. Circular RNA (circRNA), a newly identified type of non-coding RNA, is abundant within cells and can be released via exosomes, facilitating communication between cells. Studies have demonstrated that exosomal circRNAs can alter the tumor microenvironment and modulate immune responses by influencing the functions of T cells, natural killer (NK) cells, and macrophages, thereby enabling tumors to evade the immune system. Moreover, exosomal circRNAs show potential as diagnostic biomarkers and therapeutic targets for cancer. This review summarizes the regulatory roles of exosomal circRNAs in immune cells and their potential applications in cancer progression and treatment, highlighting their promise in improving cancer immunotherapy. Future research should concentrate on understanding the mechanisms of key exosomal circRNAs and developing targeted immunotherapy methods.
Costal chondrosarcoma in a woman with hereditary multiple exostoses - a case report
Authors: Yang Ze , Wang Kaiqiang , Pu Jiangtao
Published: February 2025

In this report, we present a case of a 32-year-old female previously diagnosed with hereditary multiple exostoses(HME) who was incidentally found to have an asymptomatic anterior mediastinal mass during a routine examination. Computed tomography imaging revealed a well-defined mass measuring approximately 2.3 cm x 4.0 cm x 4.7 cm in the anterior mediastinum with multiple nodular areas of high density within. The mass caused compression and narrowing of the right ventricle. The patient subsequently underwent intralesional resection of the tumor, and histopathological examination confirmed a diagnosis of well-differentiated chondrosarcoma. Given the patient’s medical history, the chondrosarcoma was suspected to have originated from malignant transformation of a rib osteochondroma. The patient received adjuvant radiotherapy postoperatively and has been followed up for one year with no evidence of recurrence. This case reports a highly rare costal chondrosarcoma secondary to hereditary multiple exostoses, located in the anterior mediastinum and compressing the right ventricle. To our knowledge, this is the first reported case of costal chondrosarcoma secondary to HME occurring in the anterior mediastinum, which requires differentiation from other common anterior mediastinal tumors.
Association between preoperative inflammatory status via CALLY index and postoperative pneumonia occurrence in resectable esophageal squamous cell carcinoma patients: a retrospective cohort study
Authors: Fan Mengtao , Zhu Yihan , Qian Long , Hu Chuanxian , Ding Hui
Published: February 2025

Background

Postoperative pneumonia significantly affects recovery and prognosis in patients with esophageal squamous cell carcinoma. The CALLY index, derived from preoperative hematological parameters, may serve as a predictive marker for such complications.

Objectives

To assess the association between preoperative inflammatory status via the CALLY index and the occurrence of postoperative pneumonia in patients with resectable ESCC.

Methods

A retrospective cohort study was conducted from January 2020 to December 2022 at The Affiliated Huai’an No. 1 People’s Hospital of Nanjing Medical University. A total of 215 patients who met inclusion criteria were analyzed. Clinical data, including CALLY indices calculated preoperatively, were collected. Propensity score matching was applied to minimize confounding biases. The predictive value of the CALLY index was assessed using receiver operating characteristic analysis, and logistic regression was used to identify factors associated with postoperative pneumonia.

Results

ROC curve analysis demonstrated the CALLY index had an area under the curve of 0.764 for predicting postoperative pneumonia, with a cutoff value of 1.97 achieving 67.69% sensitivity and 84.67% specificity. In multivariate analysis, a lower CALLY index was significantly associated with increased pneumonia risk, independent of other factors (adjusted OR = 0.66, p < 0.001). High CALLY index scores correlated with a decreased likelihood of postoperative pneumonia, reinforcing its utility as a non-invasive prognostic marker.

Conclusions

The CALLY index is a robust, independent predictor of postoperative pneumonia in patients with resectable ESCC. Preoperative assessment of this index could enhance risk stratification and guide proactive management strategies to improve postoperative outcomes.
Case report: Two cases of multiple evanescent white dot syndrome with transient night blindness
Authors: Miyake Kanna , Egawa Mariko , Mitamura Yoshinori , Yanai Ryoji
Published: February 2025

Introduction

This study aimed to report two cases of multiple evanescent white dot syndrome (MEWDS) with transient night blindness.

Case Presentation

Case 1: A 24-year-old man presented with acute visual loss and night blindness in his right eye. Examination revealed an enlarged blind spot and multiple white dots extending from the posterior pole to the peripheral retina in the right eye. Optical coherence tomography (OCT) revealed multiple disruptions of the ellipsoid zone (EZ). Full-field electroretinography (ffERG) demonstrated a more pronounced reduction in rod amplitude compared with cone amplitude in both eyes. After 3 months, the white dots, EZ disruption, and night blindness resolved spontaneously, and the ffERG amplitude normalized in the right eye. However, the enlarged blind spot persisted. Case 2: A 66-year-old woman presented with acute visual deterioration and night blindness in her right eye. The right eye exhibited an enlarged blind spot and numerous white spots widely extending from the posterior pole to the periphery. OCT revealed widespread EZ loss, and ffERG showed reduced rod and cone responses. SubTenon’s triamcinolone acetonide injection was administered, and 3 months after the injection, the night blindness, ffERG abnormalities, and EZ loss had resolved, but the enlarged blind spot remained.

Conclusion

MEWDS rarely causes transient night blindness due to extensive rod dysfunction. However, outer retinal layer damage is reversible, with night blindness typically resolving within a few months.
Contribution and expression of renal drug transporters in renal cell carcinoma
Authors: Zuo Yawen , Li Tong , Yang Shilei , Chen Xuyang , Tao Xufeng , Dong Deshi , Liu Fang , Zhu Yanna
Published: February 2025

Renal cell carcinoma (RCC) is a common substantive tumor. According to incomplete statistics, RCC incidence accounts for approximately 90% of renal malignant tumors, and is the second most prevalent major malignant tumor in the genitourinary system, following bladder cancer. Only 10%–15% of chemotherapy regimens for metastatic renal cell carcinoma (mRCC) are effective, and mRCC has a high mortality. Drug transporters are proteins located on the cell membrane that are responsible for the absorption, distribution, and excretion of drugs. Lots of drug transporters are expressed in the kidneys. Changes in carrier function weaken balance, cause disease, or modify the effectiveness of drug treatment. The changes in expression of these transporters during cancer pathology results in multi-drug resistance to cancer chemotherapy. In the treatment of RCC, the study of drug transporters helps to optimize treatment regimens, improve therapeutic effects, and reduce drug side effects. In this review, we summarize advances in the role of renal drug transporters in the genesis, progression, and treatment of RCC.
Impact of the national health insurance coverage policy on mecapegfilgrastim utilization for chemoradiotherapy-induced neutropenia in cancer patients in China: a retrospective real-world analysis
Authors: Fang Wenqing , He Jizhong , Wang Ying , Zhu Yulei , Qian Xiaodan , Su Dan , Gong Jinhong , Shang Jingjing , He Yuan , Wu Hong , Li Xin
Published: February 2025

Objective

This study aimed to evaluate mecapegfilgrastim utilization for the prophylaxis of chemotherapy-induced neutropenia in cancer patients and to assess changes caused by the National Health Insurance Coverage (NHIC) policy.

Methods

Individual patient data, including demographics, medical insurance status, cancer type, and tumor stage, were extracted from electronic medical records in an oncology specialty tertiary hospital in Jiangsu Province, China. An interrupted time series (ITS) analysis with a segmented regression model was applied to evaluate the NHIC policy’s effects, and multivariate binary logistic regression analysis was used to identify key factors influencing mecapegfilgrastim utilization.

Results

The proportion of cancer patients receiving mecapegfilgrastim increased from 8.17% before the NHIC policy implementation to 36.05% after its implementation (P < 0.001). Utilization rose abruptly following the policy intervention (β = 0.143, P < 0.001) and continued to increase significantly afterward (β = 0.011, P = 0.004). However, inequities were observed in mecapegfilgrastim usage among patient subgroups, with utilization closely associated with patients’ location, cancer type, and tumor stage after the policy implementation.

Conclusion

The NHIC policy significantly increased mecapegfilgrastim utilization, enabling more cancer patients to access this medication and effectively benefiting them. To address persistent inequities, the government should consider introducing additional measures, such as increasing the insurance reimbursement cap and separating the cost of expensive innovative anticancer medicines from hospital medical insurance budgets.
Rapid detection and classification of PC + ABS meter box materials based on laser-induced breakdown spectroscopy
Authors: Xia Yunfeng , Liang Qishuai , Xia Zhongchen , Yang Jie , Ye Jiang
Published: February 2025

Polycarbonate + Acrylonitrile-Butadiene-Styrene (PC + ABS) is a typical non-metallic material used for electric meter enclosures, characterized by its corrosion resistance, excellent insulation properties, and lightweight nature, making it widely applicable in power supply systems. However, prolonged exposure to outdoor environments often leads to performance degradation, which can affect normal usage. Therefore, developing rapid detection technologies to timely identify and rectify potential faults is particularly important. This not only ensures the stable operation of the power grid but also enhances the overall performance and efficiency of the power system. This study employs Laser-Induced Breakdown Spectroscopy (LIBS) technology and proposes a method for the rapid detection of PC + ABS materials by optimizing testing system parameters. The research utilizes LIBS data to classify different formulations of PC + ABS based on Support Vector Machine (SVM) and Self-Organizing Map (SOM) neural networks. The results indicate that the classification accuracy of SVM reached 98.4%, while the unsupervised classification through the SOM neural network maintained approximately 90% consistency among the three different samples. This method achieves rapid detection and classification of PC + ABS electric meter enclosures, providing new theoretical and practical foundations for enhancing the safety and stable operation of the power grid system.
Environmental response strategies for the spatial distribution of seed plants in Gansu
Authors: Li Zizhen , Wei Jia , Du Weibo , Huang Rong , Song Lingling , Tian Qing , Zhou Xiaolei
Published: February 2025

The interplay between plant diversity and environmental response strategies is crucial for ecosystem adaptability and stability. A central focus in modern ecology is elucidating how environmental factors shape plant diversity patterns and regulate species distributions across heterogeneous landscapes. This study employed Joint Species Distribution Model (JSDM) to quantitatively analyze the influence of environmental variables on plant spatial distributions in Gansu Province, China, while examining interspecies interactions under varying conditions. Results demonstrated that environmental factors explained 95.4% of the variance, highlighting their predominant role in determining plant distributions. Habitat type accounted for the largest share of variance (33.5%), followed by elevation (22.1%), mean annual temperature (20.3%), mean annual precipitation (15.1%), and solar radiation (4.4%). Species’ responses to environmental covariates were predominantly independent, with weak phylogenetic correlation (posterior mean: 0.17), reflecting limited ecological niche conservatism at the family level. Geographically, regions such as the northern Qilian foothills, Lanzhou-Baiyin wilderness, Loess Plateau, and Gannan Plateau exhibited negative correlations with most plant families, functioning as critical limiting or driving factors in spatial variability. Additionally, 33.7% of seed plant families showed negative correlations with light intensity, underscoring its role as a major limiting factor. Provincially, competition does not primarily constrain seed plant coexistence in Gansu. Regionally, however, pronounced differences in environmental responses were observed. In the northwest, solar radiation (37%) and precipitation (25%) were dominant drivers of plant distribution, while in the southeast, solar radiation (36.3%) and elevation (34.7%) were predominant. These findings underscore that species co-occurrence patterns are scale-dependent and influenced by regional resource availability. In resource-abundant southeastern areas, plant families displayed positive co-occurrence patterns indicative of mutualistic or symbiotic interactions, whereas resource-scarce northwestern areas experienced intensified negative co-occurrences due to heightened interspecific competition. This study highlights the critical role of environmental gradients in structuring seed plant distributions in Gansu, providing insights into the interaction of ecological adaptation and evolutionary history in shaping plant diversity. By identifying the drivers of plant distribution across heterogeneous environments, this research offers significant implications for biodiversity conservation and plant resource management strategies in Gansu Province, while contributing to a broader understanding of plant-environment dynamics in complex ecosystems.
Older men in motion: bodies, masculinities, and redefinition of identity
Authors: González-Calvo Gustavo , García-Monge Alfonso , Ramalho André , Hamdi Faten , Duarte-Mendes Pedro
Published: February 2025

Introduction

This study explores the intersection of physical exercise, masculinity, and aging in older men, examining how these elements shape identity and bodily experiences. Aging is often framed through deficit-based narratives that emphasize decline; however, this research seeks to reframe aging as a process of adaptation and opportunity. By investigating how physical activity contributes to identity reconstruction, this study aims to provide insights into the broader social and structural factors influencing older men’s engagement in exercise.

Methods

The study employs a qualitative research design, utilizing semi-structured interviews with 12 men aged 65–76. A narrative thematic analysis was conducted to examine participants’ experiences, concerns, and expectations regarding physical activity. This approach allowed for an in-depth exploration of how exercise intersects with notions of masculinity and aging, as well as the barriers and facilitators that shape participation in physical activities.

Results

The analysis identified three key themes: (1) the transformative role of exercise in fostering emotional well-being and bodily awareness, (2) the renegotiation of traditional masculinity through engagement in non-traditional physical activities, and (3) the social connections cultivated through group-based exercise programs. Additionally, the study highlights social and structural barriers, such as limited access to facilities and economic constraints, which hinder participation in physical activity.

Discussion

Findings underscore the significance of physical activity not only for maintaining health but also as a space for identity reconstruction and resistance to conventional narratives of aging. The study suggests that exercise serves as a means of empowerment, allowing older men to navigate aging in ways that challenge societal expectations. Moreover, the identification of structural barriers emphasizes the need for inclusive public health policies and targeted interventions that promote active aging. By reframing aging as a dynamic process of adaptation rather than mere decline, this research contributes to a broader understanding of how older adults experience and engage with physical activity.
Multimodal machine learning-based model for differentiating nontuberculous mycobacteria from mycobacterium tuberculosis
Authors: Li Hong-ling , Zhi Ri-zeng , Liu Hua-sheng , Wang Mei , Yu Si-jie
Published: February 2025

Objective

To develop and evaluate the effectiveness of multimodal machine learning approach for the differentiation of NTM from MTB.

Methods

The clinical data and CT images of 175 patients were retrospectively obtained. We established clinical data-based model, radiomics-based model, and multimodal (clinical plus radiomics) model gradually using 5 machine learning algorithms (Logistic, XGBoost, AdaBoost, RandomForest, and LightGBM). Optimal algorithm in each model was selected after evaluating the differentiation performance both in training and validation sets. The model performance was further verified using external new MTB and NTM patient data. Performance was also compared with the existing approaches and model.

Results

The clinical data-based model contained age, gender, and IL-6, and the RandomForest algorithm achieved the optimal learning model. Two key radiomics features of CT images were identified and then used to establish the radiomics model, finding that model from Logistic algorithm was the optimal. The multimodal model contained age, IL-6, and the 2 radiomics features, and the optimal model was from LightGBM algorithm. The optimal multimodal model had the highest AUC value, accuracy, sensitivity, and negative predictive value compared with the optimal clinical or radiomics models, and its’ favorable performance was also verified in the external test dataset (accuracy = 0.745, sensitivity = 0.900). Additionally, the performance of multimodal model was better than that of the radiologist, NGS detection, and existing machine learning model, with an increased accuracy of 26, 4, and 6%, respectively.

Conclusion

This is the first study to establish multimodal model to distinguish NTM from MTB and it performs well in differentiating them, which has the potential to aid clinical decision-making for experienced radiologists.
Physical activity: the key to life satisfaction - correlations between physical activity, sedentary lifestyle, and life satisfaction among young adults before and after the COVID-19 pandemic
Authors: Bácsné Bába Éva , Lengyel Attila , Pfau Christa , Müller Anetta , Bartha Éva Judit , Balogh Renátó , Szabados György , Bács Zoltán , Ráthonyi Gergely
Published: February 2025

COVID-19 has fundamentally altered the world, significantly affecting people’s health and quality of life. These changes may have impacted young adults’ physical activity (PA) and sedentary behavior (SB). This study aims to explore lifestyle changes among young adults, particularly focusing on the relationship between physical activity, sedentary behavior, and life satisfaction before and after the COVID-19 pandemic. To achieve this, we analyzed two surveys carried out in 2018 and 2024. A total of 799 participants were recruited for this age group, with 279 in 2018 and 520 in 2024. We employed T-tests, Pearson’s Chi-square tests, Mann–Whitney tests, and ordinal linear regression to uncover associations between physical activity and sitting time across the two periods, alongside various socio-demographic factors and predictors of life satisfaction. Our results reveal notable trends and changes in physical activity levels and sitting times among young adults in Hungary between 2018 and 2024. The most striking changes occurred in IPAQ (International Physical Activity Questionnaire) categorical scores, indicating a shift in activity levels across several demographic groups. The level of physical activity increased for both male and female respondents, residents of county seats and cities, as well as those living in relationships. In 2018, young Hungarian adults who maintained a balance of physical activity—including cycling, some home-based physical work, and limited sitting time—tended to report higher life satisfaction. In 2024, an interesting pattern emerged regarding leisure activities: individuals who engaged in very hard exercise during their free time reported varying levels of life satisfaction. Those who never engaged in very hard exercise or did so only once a week reported lower satisfaction compared to those who exercised daily. This finding aligns with existing research on the mental health benefits of regular intense physical activity. Furthermore, individuals whose physical activity was primarily work-related reported significantly lower life satisfaction compared to those whose physical activity was primarily leisure-related. This suggests that the context of physical activity is crucial in determining its impact on life satisfaction.
Beyond self-reports: serum cotinine reveals sex-and age-related differences of smoking on all-cause and disease-specific mortality
Authors: Jiang Qi , Junjun Liu , Wang Xiaochuan , Luo Li , He Gaoyan , Wu Xiaojuan , Min Qian , Long Ying , Wenjun Wang , Zhu Tao , Yao Yu
Published: February 2025

Background

It is well-known that sex and age play critical roles in smoking-related diseases and mortality. However, quantification of the extent of smoking requires self-reports in these studies, which may yield only partially accurate results. This study investigated sex-and age-related differences in the association between smoking and all-cause, cardiovascular disease, and cancer mortality by measuring serum cotinine levels.

Methods

Participants aged 20–85 years from the US National Health and Nutrition Examination Survey (1999–2018) were included. All-cause and disease-specific mortality data were obtained from publicly available user-linked mortality files. Multivariate Cox regression was performed to identify serum cotinine as an independent risk factor of mortality. Subgroup and interaction analyses were performed to investigate these sex and age differences. Smooth curve fitting was conducted to discover potential nonlinear relationships and threshold saturation effects.

Results

Sex was significantly associated with all-cause and cancer mortality. Threshold saturation effects were observed in all-cause mortality among both males and females, cancer mortality among females, and cardiovascular disease mortality among males. Age markedly associated with all-cause and cardiovascular disease mortality. Threshold saturation effects were found in cardiovascular disease mortality among younger adults and cancer mortality among the all-age population.

Conclusion

These findings suggest that there are threshold saturation effects between smoking and mortality, and sex and age differences in smoking-related mortality are inconsistent in different diseases.
Demand analysis of public sports service for older adults using Kano model
Authors: Yang Xin , Leng Zimiao , Ma Changning , Yang Guanghui
Published: February 2025

Aim

China is one of the countries with a relatively high degree of population aging in the world. Compared with other countries, China has the largest number of older adults and the fastest aging speed. In the increasingly serious situation of population aging, it has become the common desire of the whole society to actively develop sports for older adults and improve the health and quality of life of older adults. By identifying the needs of public sports services for older adults, this study ensures the sustainable improvement and meeting the needs of sports services for older adults in the future. The findings aim to improve the quality of life of older adults, enhance their physical and mental health.

Methods

Participants were from Haigang District, Changli County, and Funing District of Qinhuangdao City, Hebei Province, China. A total of 436 older adults who met the survey requirements were included. The socio-demographic characteristics questionnaire was used to investigate and analyze the needs of the participants for public sports services.

Results

Of the 23 quality attributes evaluated by participants, nine were “must quality,” two were “one-dimensional quality,” three were “attractive quality,” and nine were “indifferent quality.” The better values (satisfaction) ranged from 32.80 to 58.49%, and the worse values (dissatisfaction) ranged from 28.21 to 77.98%. In addition, the average ID of 23 service quality items was 2.25, and 12 attributes were above the average, which were high value-added attributes.

Conclusion

Based on Kano model, it is found that older adults have a positive attitude toward physical activities, and the demand for different dimensions of public sports services is different. This study provides a theoretical basis for the decision makers of public sports services for older adults to formulate corresponding policies, and provides a scientific basis for sports instructors to optimize the content of public sports services and meet the personalized and characteristic needs of public sports services for older adults which contributes to healthy aging.
The impact of physical exercise on esports players: a monitoring perspective
Authors: Kusan Muhammet , Başoğlu Burhan , Aydoğmuş Mert , Ermiş Sermin Ağralı , Sekban Gülşah , Bayraktar Mustafa Tolga , Durmuşoğlu Mehmet Vakıf , Arslanoğlu Erkal , Öz Faik , Ceylan Levent , Küçük Hamza , Şahin Fatma Neşe
Published: February 2025

Background

Esports has been defined as an organized and competitive approach to playing computer games. The esports industry has grown significantly and continues to develop in recent years. Within this period, monitoring and promoting physical exercise participation among esports players is important. The main aim of this study is to examine esports players’ participation in physical exercise. Another aim is to investigate the impact of physical exercise on body appreciation among esports players.

Method

The study involved 183 esports players (age mean: 23.26; SD: 4.30; n = 42 female, n = 141 male). Data were collected using the Personal Information Form, the International Physical Activity Questionnaire Short Form (IPAQ), and the Body Appreciation Scale.

Results

The analysis revealed significant differences in total physical activity and body appreciation scores and their sub-dimensions based on income level and years of esports licensing. Demographic factors such as gender, income level, and duration of esports participation were found to have notable effects on physical activity and body appreciation.

Discussion

Male players had higher physical activity levels and body appreciation scores than females, and individuals with higher incomes reported better body appreciation. However, an increase in the duration of esports participation led to a decrease in physical activity levels and body appreciation scores. Increasing esports players’ participation in physical activity and promoting physical exercise are recommended.
Artificial social intelligence in teamwork: how team traits influence human-AI dynamics in complex tasks
Authors: Bendell Rhyse , Williams Jessica , Fiore Stephen M. , Jentsch Florian
Published: February 2025

This study examines the integration of Artificial Social Intelligence (ASI) into human teams, focusing on how ASI can enhance teamwork processes in complex tasks. Teams of three participants collaborated with ASI advisors designed to exhibit Artificial Theory of Mind (AToM) while engaged in an interdependent task. A profiling model was used to categorize teams based on their taskwork and teamwork potential and study how these influenced perceptions of team processes and ASI advisors. Results indicated that teams with higher taskwork or teamwork potential had more positive perceptions of their team processes, with those high in both dimensions showing the most favorable views. However, team performance significantly mediated these perceptions, suggesting that objective outcomes strongly influence subjective impressions of teammates. Notably, perceptions of the ASI advisors were not significantly affected by team performance but were positively correlated with higher taskwork and teamwork potential. The study highlights the need for ASI systems to be adaptable and responsive to the specific traits of human teams to be perceived as effective teammates.
Exploring the correlation of epidemiological and clinical factors with facial injury severity scores in maxillofacial trauma: a comprehensive analysis
Authors: Michalik Weronika , Toppich Julia , Łuksza Adam , Bargiel Jakub , Gąsiorowski Krzysztof , Marecik Tomasz , Szczurowski Paweł , Wyszyńska-Pawelec Grażyna , Gontarz Michał
Published: February 2025

Introduction

The Facial Injury Severity Scale (FISS) provides a numerical value based on individual fractures that can be a valuable tool for management of maxillofacial trauma patients. The aim of this study was to evaluate the association of epidemiological and clinical factors with facial fracture patterns and their correlations with FISS.

Methods

A retrospective study was conducted based on 511 medical records from a 4-year period of patients with facial trauma who underwent open reduction internal fixation (ORIF) under general anesthesia. Fracture patterns were categorized into 3 anatomic subunits: upper, middle and lower face. Single-unit and panfacial fractures groups were analyzed separately. Data regarding demographics, hospitalization, etiology of injury, fracture site and complications were collected. The overall risk of fracture within the viscerocranium requiring an ORIF was presented in graphical form.

Results

Single-unit fractures were more typical in younger patients. There was a significant association between FISS score and traumatic etiology, hospitalization time, length of surgery in each group (p < 0.001). For panfacial fractures, FISS >6 indicated prolonged surgery (>2 h) and hospitalization (>1 week).

Discussion

Despite the questionable clinical utility of FISS, classifying maxillofacial trauma can facilitate comprehensive treatment planning and multidisciplinary collaboration, particularly in complex cases such as panfacial fractures.
Magnetic particle imaging resolution needed for magnetic hyperthermia treatment planning: a sensitivity analysis
Authors: Pawar Shreeniket , Arepally Nageshwar , Carlton Hayden , Vanname Joshua , Ivkov Robert , Attaluri Anilchandra
Published: February 2025

Purpose

Magnetic particle imaging (MPI) is a nascent tracer imaging modality that generates images from magnetic iron oxide nanoparticles (MIONs) in tissue. MPI resolution is a critical input parameter for defining the reliability of simulations-based temperature predictions for magnetic nanoparticle hyperthermia (MNPH). The objective of this study was to ascertain how spatial resolution provided by MPI data affects the reliability of predicted temperatures and thermal dose in simulations using MPI data as inputs.

Methods

Computed tomography (CT) and MPI scans obtained from a tumor injected with MIONs were co-registered to align their coordinates. Co-registered data were used to obtain geometry and volumetric heat sources for computational simulations of MNPH in phantom tumors. In addition to using the MPI-derived in vivo MION distribution (D1) we analyzed two mathematical MION distributions: uniform (D2) and Gaussian (D3). All distributions were discretized into cubic voxels and the data were imported into a commercial finite element bioheat transfer (FEBHT) software for thermal simulations. FEBHT simulations were conducted using the Pennes’ bioheat equation using four different MION specific loss power (SLP) values in the range 300–600 [W/g Fe]. The impact on predicted temperature resolution and thermal dose of spatial resolution were assessed by varying the linear voxel density (LVD) from 0.36 to 4.06 [voxel/mm]. Results were compared against the simulation with the highest LVD [4.06(voxel/mm)], where deviations in temperature of ≤ ±1 [°C] and thermal dose coverage ≤ ±5 [%] were deemed acceptable.

Results

The D3 distribution resulted in the highest predicted temperatures, followed by D1 and D2; however, in terms of thermal dose, D1 showed lowest tumor coverage, requiring higher heat output from MIONs than was required for the other distributions studied. The results of the sensitivity analysis revealed that the predicted tumor temperature increased with LVD across all tested SLP values. Additionally, we observed that the minimum acceptable LVD increased with SLP.

Conclusion

Current (preclinical small animal) MPI scanners provide sufficient spatial resolution to predict temperature to within ±1 [°C], and thermal dose coverage to within ±5 [%] for MION formulations having heat output SLP = <370 [W/g Fe]. Higher spatial resolution is needed to achieve a similar precision when MION SLP exceeds 370 [W/g Fe]. We also conclude from the results that assuming a uniform MION distribution in tissue, which has been a common practice in MNPH simulations, overestimates the SLP needed to deposit meaningful thermal dose.