bluuebunny/crossref_metadata_2025 huggingface dataset:
A Rare Case of Suicide by Ingestion of Phorate: A Case Report and a Review of the Literature
Authors: Montana Angelo , Rapisarda Venerando , Esposito Massimiliano , Amico Francesco , Cocimano Giuseppe , Nunno Nunzio Di , Ledda Caterina , Salerno Monica
Published: January 2021

Phorate is a systemic organophosphorus pesticide (OP) that acts by inhibiting cholinesterases. Recent studies have reported that long-term low/moderate exposure to OP could be correlated with impaired cardiovascular and pulmonary function and other neurological effects. A 70-year-old farmer died after an intention ingestion of a granular powder mixed with water. He was employed on a farm for over 50 years producing fruit and vegetables, and for about 20 years, he had also applied pesticides. In the last 15 years, he used phorate predominantly. The Phorate concentration detected in gastric contents was 3.29 µg/mL. Chronic exposure to phorate is experimentally studied by histopathological changes observed in the kidney. In the light of current literature, our case confirms that there is an association between renal damage and chronic exposure to phorate in a subject exposed for years to the pesticide. Autopsies and toxicological analyses play a key role in the reconstruction of the dynamics, including the cause of the death.
Reassessing the Role of Potassium in Tomato Grown with Water Shortages
Authors: De Luca Anna , Corell Mireia , Chivet Mathilde , Parrado M. Angeles , Pardo José M. , Leidi Eduardo O.
Published: January 2021

Potassium (K) is closely related to plant water uptake and use and affects key processes in assimilation and growth. The aim of this work was to find out to what extent K supply and enhanced compartmentation might improve water use and productivity when tomato plants suffered from periods of water stress. Yield, water traits, gas exchange, photosynthetic rate and biomass partition were determined. When plants suffered dehydration, increasing K supply was associated with reduction in stomatal conductance and increased water contents, but failed to protect photosynthetic rate. Potassium supplements increased shoot growth, fruit setting and yield under water stress. However, increasing the K supply could not counteract the great yield reduction under drought. A transgenic tomato line with enhanced K uptake into vacuoles and able to reach higher plant K contents, still showed poor yield performance under water stress and had lower K use efficiency than the control plants. With unlimited water supply (hydroponics), plants grown in low-K showed greater root hydraulic conductivity than at higher K availability and stomatal conductance was not associated with leaf K concentration. In conclusion, increasing K supply and tissue content improved some physiological features related to drought tolerance but did not overcome yield restrictions imposed by water stress.
Gene Expression Analysis of Mevalonate Kinase Deficiency Affected Children Identifies Molecular Signatures Related to Hematopoiesis
Authors: Pisanti Simona , Citro Marianna , Abate Mario , Caputo Mariella , Martinelli Rosanna
Published: January 2021

Mevalonate kinase deficiency (MKD) is a rare autoinflammatory genetic disorder characterized by recurrent fever attacks and systemic inflammation with potentially severe complications. Although it is recognized that the lack of protein prenylation consequent to mevalonate pathway blockade drives IL1β hypersecretion, and hence autoinflammation, MKD pathogenesis and the molecular mechanisms underlaying most of its clinical manifestations are still largely unknown. In this study, we performed a comprehensive bioinformatic analysis of a microarray dataset of MKD patients, using gene ontology and Ingenuity Pathway Analysis (IPA) tools, in order to identify the most significant differentially expressed genes and infer their predicted relationships into biological processes, pathways, and networks. We found that hematopoiesis linked biological functions and pathways are predominant in the gene ontology of differentially expressed genes in MKD, in line with the observed clinical feature of anemia. We also provided novel information about the molecular mechanisms at the basis of the hematological abnormalities observed, that are linked to the chronic inflammation and to defective prenylation. Considering the broad and unspecific spectrum of MKD clinical manifestations and the difficulty in its diagnosis, a better understanding of MKD molecular bases could be translated to the clinical level to facilitate diagnosis, and improve management and therapy.
Towards Sustainable Diets: Understanding the Cognitive Mechanism of Consumer Acceptance of Biofortified Foods and the Role of Nutrition Information
Authors: Razzaq Amar , Tang Yifan , Qing Ping
Published: January 2021

Micronutrient malnutrition, also known as hidden hunger, continues to affect more than 2 billion people globally. Biofortification, which is a process of breeding staple crops with improved micronutrient contents such as vitamin A, iron, and zinc, offers a cost-effective and sustainable solution in reducing hidden hunger. However, the success of these foods depends on consumer acceptance. In contrast to previous studies, this research focuses on the mechanism of consumer acceptance of biofortified crops that undergo physical changes (i.e., changes in appearance) after biofortification. We use data on 473 Chinese consumers collected through online surveys to examine their purchase intentions for biofortified foods that have visible (vs. invisible) nutrition traits. Using two online surveys, we conduct two studies to reveal the mechanism and antecedents of consumer acceptance of different biofortified foods. In Study 1, we find that consumer purchase intentions vary depending upon the visibility of nutrition traits in biofortified foods. Specifically, consumers exhibit a nutrition-related food neophobia (NFN) regardless of visibility of the nutrition trait in biofortified foods; and a sensory-affective food neophobia (SFN) which is only pronounced when the nutrition trait in biofortified foods is visible. The results of the mediation analysis show that for both types of biofortified foods, NFN mediates consumers’ purchasing intentions. For foods that involve visible changes after biofortification, SFN acts as an additional mediator of consumer purchase intentions. Using ordered logistic regression analysis, we find that both food neophobias have a negative impact on consumer acceptance of biofortified foods. The results of Study 2 confirm our findings and further show that nutrition information moderates the mediation of NFN and SFN, i.e., the negative impact of NFN and SFN on purchase intentions can be reduced by providing nutrition information to consumers. The results of this study have several theoretical and practical implications and are of interest to stakeholders and marketers in the promotion of biofortified foods.
Priority Setting in the Polish Health Care System According to Patients’ Perspective
Authors: Rybarczyk-Szwajkowska Anna , Rydlewska-Liszkowska Izabela
Published: January 2021

Identification of health priorities is concerned with equitable distribution of resources and is an important part of strategic planning in the health care system. The aim of this article is to describe health priorities in the Polish health care system from the patients’ perspective. The study included 533 patients hospitalized in the Lodz region. The average age of the respondents was 48.5 years and one third (36.6%) had university education. Most of the respondents (64.9%) negatively assessed the functioning of the health care system in Poland. Most of them claimed the following aspects require improvements: financing health services (85.8%), determining priorities in health care (80.3%), the role of health insurance (80.3%), and medical education (70.8%). Over 70% of the respondents agreed the role of politicians in designing and implementing health system reforms should be limited. The fact that the respondents so negatively assessed the Polish health care system implies there is a need for full discussion on redefining health priorities.
Greater Power but Not Strength Gains Using Flywheel Versus Equivolumed Traditional Strength Training in Junior Basketball Players
Authors: Stojanović Marko D. M. , Mikić Mladen , Drid Patrik , Calleja-González Julio , Maksimović Nebojša , Belegišanin Bogdan , Sekulović Veselin
Published: January 2021

The main aim of the present study was to compare the effects of flywheel strength training and traditional strength training on fitness attributes. Thirty-six well trained junior basketball players (n = 36; 17.58 ± 0.50 years) were recruited and randomly allocated into: Flywheel group (FST; n = 12), traditional strength training group (TST; n = 12) and control group (CON; n = 12). All groups attended 5 basketball practices and one official match a week during the study period. Experimental groups additionally participated in the eight-week, 1–2 d/w equivolume intervention conducted using a flywheel device (inertia = 0.075 kg·m−2) for FST or free weights (80%1 RM) for TST. Pre-to post changes in lower limb isometric strength (ISOMET), 5 and 20 m sprint time (SPR5m and SPR20m), countermovement jump height (CMJ) and change of direction ability (t-test) were assessed with analyses of variance (3 × 2 ANOVA). Significant group-by-time interaction was found for ISOMET (F = 6.40; p = 0.000), CMJ (F = 7.45; p = 0.001), SPR5m (F = 7.45; p = 0.010) and T test (F = 10.46; p = 0.000). The results showed a significantly higher improvement in CMJ (p = 0.006; 11.7% vs. 6.8%), SPR5m (p = 0.001; 10.3% vs. 5.9%) and t-test (p = 0.045; 2.4% vs. 1.5%) for FST compared to the TST group. Simultaneously, th FST group had higher improvement in ISOMET (p = 0.014; 18.7% vs. 2.9%), CMJ (p = 0.000; 11.7% vs. 0.3%), SPR5m (p = 0.000; 10.3% vs. 3.4%) and t-test (p = 0.000; 2.4% vs. 0.6%) compared to the CON group. Players from the TST group showed better results in CMJ (p = 0.006; 6.8% vs. 0.3%) and t-test (p = 0.018; 1.5% vs. 0.6%) compared to players from the CON group. No significant group-by-time interaction was found for sprint 20 m (F = 2.52; p = 0.088). Eight weeks of flywheel training (1–2 sessions per week) performed at maximum concentric intensity induces superior improvements in CMJ, 5 m sprint time and change of direction ability than equivolumed traditional weight training in well trained junior basketball players. Accordingly, coaches and trainers could be advised to use flywheel training for developing power related performance attributes in young basketball players.
Nicotinic Acetylcholine Receptor Involvement in Inflammatory Bowel Disease and Interactions with Gut Microbiota
Authors: Rueda Ruzafa Lola , Cedillo José Luis , Hone Arik J.
Published: January 2021

The gut-brain axis describes a complex interplay between the central nervous system and organs of the gastrointestinal tract. Sensory neurons of dorsal root and nodose ganglia, neurons of the autonomic nervous system, and immune cells collect and relay information about the status of the gut to the brain. A critical component in this bi-directional communication system is the vagus nerve which is essential for coordinating the immune system’s response to the activities of commensal bacteria in the gut and to pathogenic strains and their toxins. Local control of gut function is provided by networks of neurons in the enteric nervous system also called the ‘gut-brain’. One element common to all of these gut-brain systems is the expression of nicotinic acetylcholine receptors. These ligand-gated ion channels serve myriad roles in the gut-brain axis including mediating fast synaptic transmission between autonomic pre- and postganglionic neurons, modulation of neurotransmitter release from peripheral sensory and enteric neurons, and modulation of cytokine release from immune cells. Here we review the role of nicotinic receptors in the gut-brain axis with a focus on the interplay of these receptors with the gut microbiome and their involvement in dysregulation of gut function and inflammatory bowel diseases.
Risk Behaviours Associated with Dating and Relationship Violence among 11–16 Year Olds in Wales: Results from the 2019 Student Health and Wellbeing Survey
Authors: Couturiaux Danielle V. R. , Young Honor , Anthony Rebecca E. , Page Nicholas , Lowthian Emily , Melendez-Torres G. J. , Hewitt Gillian , Moore Graham F.
Published: January 2021

(1) Background: This study examines the associations between risk behaviours and adolescent emotional and physical dating and relationship violence (DRV) victimisation and perpetration, and how these vary by gender. The risk behaviours explored include bullying, cyberbullying, sexting, alcohol, and cannabis use; (2) Methods: Cross-sectional self-report data from the School Health Research Network (SHRN) 2019 Student Health Wellbeing (SHW) survey of 48,397 students aged 11–16 from 149 schools across Wales were analysed using single and multiple-behaviour logistic regression models to explore the associations between each risk behaviour and emotional and physical DRV victimisation and perpetration; (3) Results: Bivariate analyses revealed a statistically significant association between DRV and all risk behaviours. In multivariate analyses, students who reported bullying, cyberbullying, sexting, and substance use, compared to those that had not, had significantly higher odds of experiencing and perpetrating emotional and physical DRV; and (4) Conclusions: Future studies on DRV should consider a mixed-methods approach to explore the context in which DRV and risk behaviours interrelate. Results from this study indicate the possibility that prevention and intervention programmes in school settings that seek to develop healthy school environments and peer-to-peer relationships, could inadvertently reduce the occurrence of future DRV and associated risk behaviours.
Impact of Workplace Conflicts on Self-Reported Medically Certified Sickness Absence in Latvia
Authors: Lakiša Svetlana , Matisāne Linda , Gobiņa Inese , Vanadziņš Ivars , Akūlova Lāsma , Eglīte Maija , Paegle Linda
Published: January 2021

Sickness absence is one of the most important working population health indicators. It is a complex phenomenon that is investigated by health care and occupational health specialists, economists, and work psychologists. Sickness absence is used as a predictor for morbidity and mortality, but besides the health status of an individual, sickness absence is influenced by demographic, socio-economic factors, and work environment factors. Conflicts at work are a common psychosocial risk factor that can affect sickness absence. The aim of the study was to investigate the association between different types of workplace conflict and self-reported medically certified sickness absence using cross-sectional survey data pooled from four periodic national surveys—Work conditions and risks in Latvia (2006–2018). The sample is representative of the working population of Latvia, as respondents were randomly drawn from different regions and industries. In total, the study sample (n = 8557) consisted of employees between 16 and 80 years old (average 42.8 +/− 12.6) of which 46.2% were males and 53.8% were females. Researchers used the computer-assisted personal interviewing (CAPI) method for collecting data. The association between workplace conflicts and sickness absence was analysed by using binomial logistic regression and calculated as odds ratios (OR) with 95% confidence intervals (CI), with adjustment for gender, age, education and survey year. The risk of sickness absence was higher among women (OR = 1.24, CI 1.13–1.35), employees aged 25–44 years old and employees with higher income. Controlling for socio-demographic factors and survey year, the odds of sickness absence increased significantly for all types of workplace conflict analysed. The strongest association with sickness absence was related to conflicts between managers and employees (OR = 1.51, CI 1.37–1.66) and conflicts between groups of employees (OR = 1.45, CI 1.31–1.61). Conflicts between employees and with customers also increased the odds of sickness absence (OR = 1.39, CI 1.27–1.52 and OR = 1.11, CI 1.01–1.23, respectively). Our findings suggest that tailored interventions at a company level for reducing workplace conflicts as risk factors of sickness absence are required. Those should focus on the improvement of managers’ leadership and human resource management skills.
A Ridesharing Choice Behavioral Equilibrium Model with Users of Heterogeneous Values of Time
Authors: Li Xingyuan , Bai Jing
Published: January 2021

Travelers decide whether to participate in ridesharing based on the trade-off between the travel time and the expense. However, it is still unclear how travelers’ values of time affect their ridesharing behaviors on the congested network. To this end, a path-based ridesharing traffic assignment model was proposed by considering travelers’ heterogenous values of time. In the proposed model, travelers are divided into several classes according to their values of time, and travelers in each class choose their travel modes and routes simultaneously which cost the least. Moreover, travelers in different classes could share the same vehicle to complete their trips together in the proposed model. This paper further discusses how the high-occupancy toll lane affects travelers’ ridesharing behaviors. Numerical results show that: (1) travelers with different values of time show differences in their ridesharing behavior; (2) the single-class ridesharing traffic assignment model may miscalculate the ridesharing scale of users; and (3) building high-occupancy toll lanes plays a positive role in promoting ridesharing for travelers with heterogeneous values of time.
Biases in the Detection of Intentionally Poisoned Animals: Public Health and Conservation Implications from a Field Experiment
Authors: Gil-Sánchez José M. , Aguilera-Alcalá Natividad , Moleón Marcos , Sebastián-González Esther , Margalida Antoni , Morales-Reyes Zebensui , Durá-Alemañ Carlos J. , Oliva-Vidal Pilar , Pérez-García Juan M. , Sánchez-Zapata José A.
Published: January 2021

Intentional poisoning is a global wildlife problem and an overlooked risk factor for public health. Managing poisoning requires unbiased and high-quality data through wildlife monitoring protocols, which are largely lacking. We herein evaluated the biases associated with current monitoring programmes of wildlife poisoning in Spain. We compared the national poisoning database for the 1990–2015 period with information obtained from a field experiment during which we used camera-traps to detect the species that consumed non-poisoned baits. Our findings suggest that the detection rate of poisoned animals is species-dependent: Several animal groups (e.g., domestic mammalian carnivores and vultures) tended to be over-represented in the poisoning national database, while others (e.g., corvids and small mammals) were underrepresented. As revealed by the GLMM analyses, the probability of a given species being overrepresented was higher for heaviest, aerial, and cryptic species. In conclusion, we found that monitoring poisoned fauna based on heterogeneous sources may produce important biases in detection rates; thus, such information should be used with caution by managers and policy-makers. Our findings may guide to future search efforts aimed to reach a more comprehensive understanding of the intentional wildlife poisoning problem.
Latent Variables Quantifying Neighborhood Characteristics and Their Associations with Poor Mental Health
Authors: Forthman Katherine L. , Colaizzi Janna M. , Yeh Hung-wen , Kuplicki Rayus , Paulus Martin P.
Published: January 2021

Neighborhood characteristics can have profound impacts on resident mental health, but the wide variability in methodologies used across studies makes it difficult to reach a consensus as to the implications of these impacts. The aim of this study was to simplify the assessment of neighborhood influence on mental health. We used a factor analysis approach to reduce the multi-dimensional assessment of a neighborhood using census tracts and demographic data available from the American Community Survey (ACS). Multivariate quantitative characterization of the neighborhood was derived by performing a factor analysis on the 2011–2015 ACS data. The utility of the latent variables was examined by determining the association of these factors with poor mental health measures from the 500 Cities Project 2014–2015 data (2017 release). A five-factor model provided the best fit for the data. Each factor represents a complex multi-dimensional construct. However, based on heuristics and for simplicity we refer to them as (1) Affluence, (2) Singletons in Tract, (3) African Americans in Tract, (4) Seniors in Tract, and (5) Hispanics or Latinos in Tract. African Americans in Tract (with loadings showing larger numbers of people who are black, single moms, and unemployed along with fewer people who are white) and Affluence (with loadings showing higher income, education, and home value) were strongly associated with poor mental health (R2=0.67, R2=0.83). These findings demonstrate the utility of this factor model for future research focused on the relationship between neighborhood characteristics and resident mental health.
Identification of Growth Patterns in Low Birth Weight Infants from Birth to 5 Years of Age: Nationwide Korean Cohort Study
Authors: Yoon So Jin , Lim Joohee , Han Jung Ho , Shin Jeong Eun , Lee Soon Min , Eun Ho Seon , Park Min Soo , Park Kook In
Published: January 2021

This study aimed to investigate the nationwide growth pattern of infants in Korea according to the birth-weight group and to analyze the effect of growth on development. A total of 430,541 infants, born in 2013 and who received the infant health check-up regularly from 6 months to 60 months of age, were included. The weight, height, head circumferences percentiles, and neurodevelopment using screening tests results were compared among the birth-weight groups. Using longitudinal analysis, the study found a significant difference in height, weight, and head circumference, respectively, according to age at health check-up, birth weight group, and combination of age and birth weight (p < 0.001). The growth parameters at 60 months of age showed a significant correlation with those at 6 months of age especially in extremely low birth weight infants. The incidence of suspected developmental delay was significantly higher in infants with growth below the 10th percentiles than in those with growth above the 10th percentiles. Among 4571 (1.6%) infants with suspected developmental delay results at 60 months of age, birth weight, sex, and poor growth parameters were confirmed as associated factors. This nationwide Korean study shows that poor growth and neurodevelopment outcomes persisted among low-birth-weight infants at 60 months of age. Our findings provide guidance for developing a nationwide follow-up program for infants with perinatal risk factors in Korea.
Household Cooking and Eating out: Food Practices and Perceptions of Salt/Sodium Consumption in Costa Rica
Authors: Blanco-Metzler Adriana , Núñez-Rivas Hilda , Vega-Solano Jaritza , Montero-Campos María A. , Benavides-Aguilar Karla , Cubillo-Rodríguez Nazareth
Published: January 2021

This research aims to study the food practices and perceptions related to excessive consumption of salt/sodium when cooking and eating outside the home in a study population representing the wide intergenerational and sociocultural diversity of Costa Rica. Key communities from around the country, cultural experts, and key informants were selected. Four qualitative research techniques were applied. Data was systematized based on the Social Ecological Model. Women are generally in charge of cooking and family food purchases. Salt is perceived as a basic ingredient, used in small amounts that can be reduced—but not eliminated—when cooking. Changes in food preparations and emotions associated with the consumption of homemade food with salt were identified. The population likes to eat out, where the establishments selected depend mainly on age group and income. Beyond cultural and geographical differences, age aspects are suggested as being the main differentiators, in terms of use of salt, seasonings, and condiments in the preparation of food at home, the recipes prepared, and the selection of establishments in which to eat out. The deeply rooted values and meanings associated with salt in food indicate that the implementation of salt reduction strategies in Costa Rica is challenging.
National Surveillance of Injury in the Republic of Korea: Increased Injury Vulnerability in the Late Middle Age
Authors: Chang Hansol , Min Ji Young , Yoo Dajeong , Lee Se Uk , Hwang Sung Yeon , Yoon Hee , Cha Won Chul , Shin Tae Gun , Jo Ik Joon , Kim Taerim
Published: January 2021

Surveillance of injury patterns and comparisons among different age groups help develop a better understanding of recent injury trends and early prevention. This study conducted a national surveillance of injury by age group. Data were collected retrospectively from Emergency Department-Based Injury In-Depth Surveillance (EDIIS) in South Korea, between January 2011 and December 2017. Patients were divided into the following four groups by age: Group 1–18 to 34 years, Group 2–35 to 49 years, Group 3–50 to 64 years, and Group 4—≥65 years. A total of 1,221,746 patients were included in the study. Findings revealed that, each year, the injury rate increased in the population aged ≥65 years. The place and mechanism of injury in Group 3 were similar to those in younger age groups, while injury outcomes and injured body parts were similar to those in Group 4. Further, hospital admission rate, ICU admission rate, hospital death, traumatic brain injury, and injury severity increased with an increase in age. In our study, each age group showed diverse characteristics pertaining to the mechanism, place, time, and outcomes of injuries. Interestingly, Group 3, which represented the late middle age, exhibited increased vulnerability to injury, and emerged as a gray zone between the young and old age groups. Therefore, different injury prevention methods are needed for each age group. Specifically, early prevention methods need to be implemented from the late middle age to improve the old age group’s injury outcomes.
Examining the External Antecedents of Innovative Work Behavior: The Role of Government Support for Talent Policy
Authors: Zhang Zaisheng , Liu Meng , Yang Qing
Published: January 2021

The innovative work behavior (IWB) or creativity of employees is regarded as the key to the sustainable innovation performance of an organization. In the field of human resource management (HRM), the relationship between an organization’s high-performance work system (HPWS) and IWB has been studied extensively. However, the current understanding of organizational external antecedents is limited. Our paper focuses on an extra-organizational government support factor, government support for talent policy (GSTP). Similar to HRM policies within an organization, GSTP also has an ability–motivation–opportunity framework that may influence the IWB of employees. We integrate the resource dependence theory, institutional theory, and theory of planned behavior (TPB) to propose a theoretical model of the mechanism of GSTP influence on IWB. Using a structural equation modeling approach, we empirically verify the hypotheses in a survey dataset of HRM practitioners in 152 technology-based enterprises in China. The results indicate that the external antecedent, GSTP, positively influences the innovative attitude, subjective norm, and perceived behavioral control of HRM practitioners in the organization. Innovative attitude and perceived behavioral control completely mediated the relationship between GSTP and innovative intention. Moreover, there is a distal indirect effect between GSTP and IWB. The paper contributes to filling a gap in the innovation policy literature. In practice, both HPWS and individual employees should be concerned about the possible role of GSTP.
Alcohol Consumption during a Pandemic Lockdown Period and Change in Alcohol Consumption Related to Worries and Pandemic Measures
Authors: Alpers Silvia Eiken , Skogen Jens Christoffer , Mæland Silje , Pallesen Ståle , Rabben Åsgeir Kjetland , Lunde Linn-Heidi , Fadnes Lars Thore
Published: January 2021

Whether lockdown related to the COVID-19 pandemic influences alcohol consumption is not well known. This study assesses alcohol consumption and hazardous drinking behavior during the initial phase of pandemic measures in Norway and identifies potential risk factors. A cross-sectional study (N = 25,708) was conducted in Bergen, Norway, following the first six weeks of strict infection control measures. In a model of self-assessed increased alcohol consumption, logistic regression analysis was conducted with independent variables for COVID-19-related worries, joblessness, quarantine, self-reported drinking behavior, age, gender, and occupational situation. These are reported with odds ratios (ORs) with 95% confidence intervals. Fifty-one percent of respondents reported economic or health-related worries due to COVID-19, 16% had been in quarantine, 49% worked/studied from home, 54% reported hazardous drinking behavior, and 13% reported increased alcohol consumption. People aged 30–39 years had elevated odds of increased alcohol consumption during lockdown (OR 3.1, 2.4−3.8) compared to the oldest adults. Increased drinking was more frequent among people reporting economic worries (OR 1.6, 1.4−1.8), those quarantined (OR 1.2, 1.1−1.4), and those studying or working at home (OR 1.4, 1.3−1.6). More than half of respondents reported hazardous drinking behavior. Increased alcohol consumption during lockdown was common among people with economic worries, people in quarantine, and people studying or working at home. These data could be important when adjusting pandemic measures.
Pausing Mid-Sentence: An Ecological Model Approach to Language Disorder and Lived Experience of Young Male Offenders
Authors: Fitzsimons Dermot , Clark Ann
Published: January 2021

International research evidence has firmly established a high prevalence of language disorder in young offender populations. Less is known about young offenders’ perspectives on their own language abilities. The study recruited an opportunity sample of 10 young men in custody at a Scottish youth offending institution who had recent experience of segregation. This mixed-methods study investigated participants’ views on their language and communication abilities to inform future support and intervention, and formal language assessment was also administered to investigate indicative prevalence of language disorder within the sample. It focused on their communication with professionals and peers in justice, education and welfare settings. Results of standardised language assessment indicated the presence of language disorder in 44% (n = 4) of the sample (n = 9). Thematic analysis of interview data led to formulation of three themes: Valuing Communication, Literacy and Learning; Exerting Control; and Seeking Support. The first theme is discussed with reference to Bronfenbrenner’s Bioecological Model. Participants offered reflective and rich views on their lived experience. They provided perspectives on features of successful interaction with peers and authority figures, importance of effective communication and the difficulties they encountered. This study argues for additional communication support for young people in the justice system.
Analysis of the Chemical and Physical Environmental Aspects that Promoted the Spread of SARS-CoV-2 in the Lombard Area
Authors: Dragone Roberto , Licciardi Giorgio , Grasso Gerardo , Del Gaudio Costantino , Chanussot Jocelyn
Published: January 2021

Recent works have demonstrated that particulate matter (PM) and specific meteorological conditions played an important role in the airborne transmission of the SARS-CoV-1 and MERS-CoV. These studies suggest that these parameters could influence the transmission of SARS-CoV-2. In the present investigation, we sought to investigate the association between air pollution, meteorological data, and the Lombardy region COVID-19 outbreak caused by SARS-CoV-2. We considered the number of detected infected people at the regional and provincial scale from February to March 2020. Air pollution data were collected over the Lombardy region, nominally, sulphur dioxide, ammonia, nitrogen dioxide, nitrogen monoxide, carbon monoxide, ozone, and suspended particulate matter measuring less than 10 μm (PM10) and less than 2.5 μm (PM2.5). Meteorological data have been collected over the same region for temperature, relative humidity, and wind speed. In this work, we evaluated the combined impact of environmental pollutants and climate conditions on the COVID-19 outbreak. The analysis evidenced a positive correlation between spatial distribution of COVID-19 infection cases with high concentrations of suspended particulate matter and a negative relationship with ozone. Moreover, suspended particulate matter concentration peaks in February correlated positively with infection peaks according to the virus incubation period. The obtained results suggested that seasonal weather conditions and concentration of air pollutants seemed to influence COVID-19 epidemics in Lombardy region.
Adiponectin as a Potential Biomarker for Pregnancy Disorders
Authors: Pheiffer Carmen , Dias Stephanie , Jack Babalwa , Malaza Nompumelelo , Adam Sumaiya
Published: January 2021

Adiponectin is an adipocyte-derived hormone that plays a critical role in energy homeostasis, mainly attributed to its insulin-sensitizing properties. Accumulating studies have reported that adiponectin concentrations are decreased during metabolic diseases, such as obesity and type 2 diabetes, with an emerging body of evidence providing support for its use as a biomarker for pregnancy complications. The identification of maternal factors that could predict the outcome of compromised pregnancies could act as valuable tools that allow the early recognition of high-risk pregnancies, facilitating close follow-up and prevention of pregnancy complications in mother and child. In this review we consider the role of adiponectin as a potential biomarker of disorders associated with pregnancy. We discuss common disorders associated with pregnancy (gestational diabetes mellitus, preeclampsia, preterm birth and abnormal intrauterine growth) and highlight studies that have investigated the potential of adiponectin to serve as biomarkers for these disorders. We conclude the review by recommending strategies to consider for future research.
A DNA Sequence Based Polymer Model for Chromatin Folding
Authors: Zhou Rui , Gao Yi Qin
Published: January 2021

The recent development of sequencing technology and imaging methods has provided an unprecedented understanding of the inter-phase chromatin folding in mammalian nuclei. It was found that chromatin folds into topological-associated domains (TADs) of hundreds of kilo base pairs (kbps), and is further divided into spatially segregated compartments (A and B). The compartment B tends to be located near to the periphery or the nuclear center and interacts with other domains of compartments B, while compartment A tends to be located between compartment B and interacts inside the domains. These spatial domains are found to highly correlate with the mosaic CpG island (CGI) density. High CGI density corresponds to compartments A and small TADs, and vice versa. The variation of contact probability as a function of sequential distance roughly follows a power-law decay. Different chromosomes tend to segregate to occupy different chromosome territories. A model that can integrate these properties at multiple length scales and match many aspects is highly desired. Here, we report a DNA-sequence based coarse-grained block copolymer model that considers different interactions between blocks of different CGI density, interactions of TAD formation, as well as interactions between chromatin and the nuclear envelope. This model captures the various single-chromosome properties and partially reproduces the formation of chromosome territories.
How to Get Them off?—Assessment of Innovative Techniques for Generation and Detachment of Mature Osteoclasts for Biomaterial Resorption Studies
Authors: Heinemann Christiane , Adam Josephine , Kruppke Benjamin , Hintze Vera , Wiesmann Hans-Peter , Hanke Thomas
Published: January 2021

The fusion process of mononuclear monocytes into multinuclear osteoclasts in vitro is an essential process for the study of osteoclastic resorption of biomaterials. Thereby biomaterials offer many influencing factors such as sample shape, material composition, and surface topography, which can have a decisive influence on the fusion and thus the entire investigation. For the specific investigation of resorption, it can therefore be advantageous to skip the fusion on samples and use mature, predifferentiated osteoclasts directly. However, most conventional detachment methods (cell scraper, accutase), lead to a poor survival rate of osteoclasts or to a loss of function of the cells after their reseeding. In the present study different conventional and novel methods of detachment in combination with different culture surfaces were investigated to obtain optimal osteoclast differentiation, yield, and vitality rates without loss of function. The innovative method—using thermoresponsive surfaces for cultivation and detachment—was found to be best suited. This is in particular due to its ability to maintain osteoclast activity, as proven by TRAP 5b-, CTSK-activity and resorption pits on dentin discs and decellularized osteoblast-derived matrix plates. In conclusion, it is shown, that osteoclasts can be predifferentiated on cell culture dishes and transferred to a reference biomaterial under preservation of osteoclastic resorption activity, providing biomaterial researchers with a novel tool for material characterization.
Reorganization of Metabolism during Cardiomyogenesis Implies Time-Specific Signaling Pathway Regulation
Authors: Barisón María Julia , Pereira Isabela Tiemy , Waloski Robert Anny , Dallagiovanna Bruno
Published: January 2021

Understanding the cell differentiation process involves the characterization of signaling and regulatory pathways. The coordinated action involved in multilevel regulation determines the commitment of stem cells and their differentiation into a specific cell lineage. Cellular metabolism plays a relevant role in modulating the expression of genes, which act as sensors of the extra-and intracellular environment. In this work, we analyzed mRNAs associated with polysomes by focusing on the expression profile of metabolism-related genes during the cardiac differentiation of human embryonic stem cells (hESCs). We compared different time points during cardiac differentiation (pluripotency, embryoid body aggregation, cardiac mesoderm, cardiac progenitor and cardiomyocyte) and showed the immature cell profile of energy metabolism. Highly regulated canonical pathways are thoroughly discussed, such as those involved in metabolic signaling and lipid homeostasis. We reveal the critical relevance of retinoic X receptor (RXR) heterodimers in upstream retinoic acid metabolism and their relationship with thyroid hormone signaling. Additionally, we highlight the importance of lipid homeostasis and extracellular matrix component biosynthesis during cardiomyogenesis, providing new insights into how hESCs reorganize their metabolism during in vitro cardiac differentiation.
The Cardiovascular Phenotype in Fabry Disease: New Findings in the Research Field
Authors: Sorriento Daniela , Iaccarino Guido
Published: January 2021

Fabry disease (FD) is a lysosomal storage disorder, depending on defects in alpha-galactosidase A (GAL) activity. At the clinical level, FD shows a high phenotype variability. Among them, cardiovascular dysfunction is often recurrent or, in some cases, is the sole symptom (cardiac variant) representing the leading cause of death in Fabry patients. The existing therapies, besides specific symptomatic treatments, are mainly based on the restoration of GAL activity. Indeed, mutations of the galactosidase alpha gene (GLA) cause a reduction or lack of GAL activity leading to globotriaosylceramide (Gb3) accumulation in several organs. However, several other mechanisms are involved in FD’s development and progression that could become useful targets for therapeutics. This review discusses FD’s cardiovascular phenotype and the last findings on molecular mechanisms that accelerate cardiac cell damage.
Arginine Methylation Regulates Ribosome CAR Function
Authors: Scopino Kristen , Dalgarno Carol , Nachmanoff Clara , Krizanc Daniel , Thayer Kelly M. , Weir Michael P.
Published: January 2021

The ribosome CAR interaction surface is hypothesized to provide a layer of translation regulation through hydrogen-bonding to the +1 mRNA codon that is next to enter the ribosome A site during translocation. The CAR surface consists of three residues, 16S/18S rRNA C1054, A1196 (E. coli 16S numbering), and R146 of yeast ribosomal protein Rps3. R146 can be methylated by the Sfm1 methyltransferase which is downregulated in stressed cells. Through molecular dynamics analysis, we show here that methylation of R146 compromises the integrity of CAR by reducing the cation-pi stacking of the R146 guanidinium group with A1196, leading to reduced CAR hydrogen-bonding with the +1 codon. We propose that ribosomes assembled under stressed conditions have unmethylated R146, resulting in elevated CAR/+1 codon interactions, which tunes translation levels in response to the altered cellular context.
Laccase-Catalyzed 1,4-Dioxane-Mediated Synthesis of Belladine N-Oxides with Anti-Influenza A Virus Activity
Authors: Zippilli Claudio , Botta Lorenzo , Bizzarri Bruno Mattia , Nencioni Lucia , De Angelis Marta , Protto Virginia , Giorgi Gianluca , Baratto Maria Camilla , Pogni Rebecca , Saladino Raffaele
Published: January 2021

Belladine N-oxides active against influenza A virus have been synthetized by a novel laccase-catalyzed 1,4-dioxane-mediated oxidation of aromatic and side-chain modified belladine derivatives. Electron paramagnetic resonance (EPR) analysis confirmed the role of 1,4-dioxane as a co-oxidant. The reaction was chemo-selective, showing a high functional-group compatibility. The novel belladine N-oxides were active against influenza A virus, involving the early stage of the virus replication life cycle.
Main Applications of Cyclodextrins in the Food Industry as the Compounds of Choice to Form Host–Guest Complexes
Authors: Gonzalez Pereira Antía , Carpena Maria , García Oliveira Paula , Mejuto Juan Carlos , Prieto Miguel Angel , Simal Gandara Jesus
Published: January 2021

Cyclodextrins (CDs) are cyclic oligomers broadly used in food manufacturing as food additives for different purposes, e.g., to improve sensorial qualities, shelf life, and sequestration of components. In this review, the latest advancements of their applications along with the characteristics of the uses of the different CDs (α, β, γ and their derivatives) were reviewed. Their beneficial effects can be achieved by mixing small amounts of CDs with the target material to be stabilized. Essentially, they have the capacity to form stable inclusion complexes with sensitive lipophilic nutrients and constituents of flavor and taste. Their toxicity has been also studied, showing that CDs are innocuous in oral administration. A review of the current legislation was also carried out, showing a general trend towards a wider acceptance of CDs as food additives. Suitable and cost-effective procedures for the manufacture of CDs have progressed, and nowadays it is possible to obtain realistic prices and used them in foods. Therefore, CDs have a promising future due to consumer demand for healthy and functional products.
Establishment and Long-Term Expansion of Small Cell Lung Cancer Patient-Derived Tumor Organoids
Authors: Choi Seon Young , Cho Yong-Hee , Kim Da-Som , Ji Wonjun , Choi Chang-Min , Lee Jae Cheol , Rho Jin Kyung , Jeong Gi Seok
Published: January 2021

Differential chemo-sensitivity of cancer cells, which is attributed to the cellular heterogeneity and phenotypic variation of cancer cells, is considered to be the main reason for tumor recurrence after chemotherapy. Here, we generated small cell lung cancer patient-derived tumor organoids and subjected them to long-term expansion with the addition of WNT3A or R-spondin1. We confirmed that the organoids have similar genetic profiles, molecular characteristics, and morphological architectures to the corresponding patient tumor tissue during and after long-term expansion. Interestingly, the cellular heterogeneity of organoids is reflected in their differential response to cisplatin or etoposide. We propose to utilize the organoids as small cell lung cancer patient avatar models that would be ideal for investigating the mechanisms underlying tumor recurrence after chemotherapy, and would ultimately help to develop personalized medicine.
Molecular Characterization of Paralichthys olivaceus MAF1 and Its Potential Role as an Anti-Viral Hemorrhagic Septicaemia Virus Factor in Hirame Natural Embryo Cells
Authors: Kim Julan , Cho Ja Young , Kim Ju-Won , Kim Dong-Gyun , Nam Bo-Hye , Kim Bong-Seok , Kim Woo-Jin , Kim Young-Ok , Cheong JaeHun , Kong Hee Jeong
Published: January 2021

MAF1 is a global suppressor of RNA polymerase III-dependent transcription, and is conserved from yeast to human. Growing evidence supports the involvement of MAF1 in the immune response of mammals, but its biological functions in fish are unknown. We isolated and characterized Maf1 from the olive flounder Paralichthys olivaceus (PoMaf1). The coding region of PoMaf1 comprised 738 bp encoding a 245-amino-acid protein. The deduced PoMAF1 amino acid sequence shared features with those of MAF1 orthologues from vertebrates. PoMaf1 mRNA was detected in all tissues examined, and the levels were highest in eye and muscle tissue. The PoMaf1 mRNA level increased during early development. In addition, the PoMaf1 transcript level decreased during viral hemorrhagic septicemia virus (VHSV) infection of flounder hirame natural embryo (HINAE) cells. To investigate the role of PoMaf1 in VHSV infection, single-cell-derived PoMaf1 knockout HINAE cells were generated using the clustered regularly interspaced short palindromic repeats/CRISPR-associated-9 (CRISPR/Cas9) system, and cell clones with complete disruption of PoMaf1 were selected. PoMaf1 disruption increased the VHSV glycoprotein (G) mRNA levels during VHSV infection of HINAE cells, implicating PoMAF1 in the immune response to VSHV infection. To our knowledge, this is the first study to characterize fish Maf1, which may play a role in the response to viral infection.