bluuebunny/crossref_metadata_2025 huggingface dataset:
Misreported Cancer Deaths in PLATO Trial
Authors: Serebruany Victor , Tanguay Jean-Francois
Published: July 2021

The potential link between antiplatelet agents and anticoagulants with excess cancer deaths (CD) was reported first for prasugrel (TRITON, DAPT), clopidogrel (DAPT), vorapaxar (TRACER), apixaban (APPRAISE-2), and later ticagrelor (PEGASUS). However, verified CD in the ticagrelor indication-seeking PLATO were not public. We obtained the complete list of deaths and their primary causes in PLATO, matched that dataset against local site records, and analyzed the patterns of CD reporting. The FDA-issued spreadsheet contains 31 precisely detailed CD (PLATO code 12-3). We obtained local site evidence for four CD and matched them with FDA-reported. We also assessed the patterns of how CD were reported among non-vascular death database column “S” by scrolling the FDA Excel file down among 938 PLATO entries. Clopidogrel CD (n = 17) were reported exclusively by sponsor, while independent CRO’s reported only ticagrelor CD (3 out of 14 PLATO total). Among four matched verified outcomes, one ticagrelor CD was correct, second ticagrelor CD was unreported, and two (ticagrelor and clopidogrel) CD were reported inaccurately. Of the remaining 16 clopidogrel CD six were reported as three separate next in line paired entries in Denmark (236–237), Poland (597–598), Romania (679–680), and as two more fatalities in South Africa (786) and Spain (789), while patients 787 and 788 received ticagrelor out of 938 records suggesting possible late addition of incorrect clopidogrel CD reports. We conclude that some CD were misreported in PLATO, favoring ticagrelor. Such mismatch may require reevaluation of this critical outcome in the trial focusing on the exact death cause reported by site investigators.
It Requires More Than Intelligence to Solve Consequential World Problems
Authors: Funke Joachim
Published: July 2021

What are consequential world problems? As “grand societal challenges”, one might define them as problems that affect a large number of people, perhaps even the entire planet, including problems such as climate change, distributive justice, world peace, world nutrition, clean air and clean water, access to education, and many more. The “Sustainable Development Goals”, compiled by the United Nations, represent a collection of such global problems. From my point of view, these problems can be seen as complex. Such complex problems are characterized by the complexity, connectivity, dynamics, intransparency, and polytely of their underlying systems. These attributes require special competencies for dealing with the uncertainties of the given domains, e.g., critical thinking. My position is that it is not IQ, but complex problem-solving competencies for dealing with complex and dynamic situations, that is important for handling consequential global problems. These problems require system competencies, i.e., competencies that go beyond analytical intelligence, and comprise systems understanding as well as systems control. Complex problem solving is more than analytic intelligence.
Individualized Prognostic Prediction of the Long-Term Functional Trajectory in Pediatric Acquired Brain Injury
Authors: Molteni Erika , Ranzini Marta Bianca Maria , Beretta Elena , Modat Marc , Strazzer Sandra
Published: July 2021

In pediatric acquired brain injury, heterogeneity of functional response to specific rehabilitation treatments is a key confound to medical decisions and outcome prediction. We aimed to identify patient subgroups sharing comparable trajectories, and to implement a method for the early prediction of the long-term recovery course from clinical condition at first discharge. 600 consecutive patients with acquired brain injury (7.4 years ± 5.2; 367 males; median GCS = 6) entered a standardized rehabilitation program. Functional Independent Measure scores were measured yearly, until year 7. We classified the functional trajectories in clusters, through a latent class model. We performed single-subject prediction of trajectory membership in cases unseen during model fitting. Four trajectory types were identified (post.prob. > 0.95): high-start fast (N = 92), low-start fast (N = 168), slow (N = 130) and non-responders (N = 210). Fast responders were older (chigh = 1.8; clow = 1.1) than non-responders and suffered shorter coma (chigh = −14.7; clow = −4.3). High-start fast-responders had shorter length of stay (c = −1.6), and slow responders had lower incidence of epilepsy (c = −1.4), than non-responders (p < 0.001). Single-subject trajectory could be predicted with high accuracy at first discharge (accuracy = 0.80). In conclusion, we stratified patients based on the evolution of their response to a specific treatment program. Data at first discharge predicted the response over 7 years. This method enables early detection of the slow responders, who show poor post-acute functional gains, but achieve recovery comparable to fast responders by year 7. Further external validation in other rehabilitation programs is warranted.
Molecular Pathology of Cancer: The Past, the Present, and the Future
Authors: Müllauer Leonhard
Published: July 2021

Clinical pathology developed from the study of macroscopic organ and tissue changes at autopsies [...]
Italian-Bangladeshis in London: Onward Migration and Its Effects on Their Linguistic Repertoire
Authors: Goglia Francesco
Published: July 2021

This article discussed language use and language maintenance among the Italian-Bangladeshi community in London, considering in particular the effects of onward migration on the reorganisation of their linguistic repertoire. Drawing on focus groups and interviews with the second-generation members of Italian-Bangladeshi families, initial findings revealed that Italian is maintained through communication with same-age friends and siblings, with older siblings acting as the main agents of language maintenance. English is considered the most important language and, together with a British education, functions as a pull-factor for onward migration to improve the second generation’s future prospects. Bengali, on the other hand, is spoken by parents among themselves and children are not always fluent in the language. Bengali also represents a marker of identity for the Italian-Bangladeshi community as opposed to the larger Sylheti-speaking British-Bangladeshi community.
Blaise Pascal’s Mechanical Calculator: Geometric Modelling and Virtual Reconstruction
Authors: Rojas-Sola José Ignacio , del Río-Cidoncha Gloria , Fernández-de la Puente Sarriá Arturo , Galiano-Delgado Verónica
Published: July 2021

This article shows the three-dimensional (3D) modelling and virtual reconstruction of the first mechanical calculating machine used for accounting purposes designed by Blaise Pascal in 1642. To obtain the 3D CAD (computer-aided design) model and the geometric documentation of said invention, CATIA V5 R20 software has been used. The starting materials for this research, mainly the plans of this arithmetic machine, are collected in the volumes Oeuvres de Blaise Pascal published in 1779. Sketches of said machine are found therein that lack scale, are not dimensioned and certain details are absent; that is, they were not drawn with precision in terms of their measurements and proportions, but they do provide qualitative information on the shape and mechanism of the machine. Thanks to the three-dimensional modelling carried out; it has been possible to explain in detail both its operation and the final assembly of the invention, made from the assemblies of its different subsets. In this way, the reader of the manuscript is brought closer to the perfect understanding of the workings of a machine that constituted a major milestone in the technological development of the time.
Non-Alcoholic Fatty Liver Disease in Children
Authors: Brecelj Jernej , Orel Rok
Published: July 2021

Background and Objectives: The prevalence of pediatric non-alcoholic fatty liver disease is increasing. A lot of new data are published regularly. Materials and Methods: Original clinical studies, review articles, and guidelines in children were searched for and the most relevant included in this review. Results: A total of 138 retrieved papers were classified into pathogenesis, epidemiology, diagnosis, and treatment. Pathogenesis is currently explained with the “multi hit hypothesis”, with complex interactions of genetic and environmental factors which trigger inflammation in steatotic liver. The prevalence is rising. A diagnosis can be made with laboratory tests, imaging, and liver biopsy after the exclusion of other causes of liver steatosis. The mainstay of treatment is lifestyle modification consisting of dietary intervention and increased physical activity. The progression to liver cirrhosis can occur even in children. Conclusions: Non-alcoholic fatty liver disease in children is a part of a metabolic syndrome in the majority of patients. Due to its complex etiology and high prevalence, multidisciplinary teams, together with public health professionals, should be involved in its treatment.
Emergence and Spread of B.1.1.7 Lineage in Primary Care and Clinical Impact in the Morbi-Mortality among Hospitalized Patients in Madrid, Spain
Authors: Martínez-García Laura , Espinel Marco Antonio , Abreu Melanie , González-Alba José María , Gijón Desirèe , McGee Amaranta , Cantón Rafael , Galán Juan Carlos , Aranaz Jesús
Published: July 2021

In December 2020, UK authorities warned of the rapid spread of a new SARS-CoV-2 variant, belonging to the B.1.1.7 lineage, known as the Alpha variant. This variant is characterized by 17 mutations and 3 deletions. The deletion 69–70 in the spike protein can be detected by commercial platforms, allowing its real-time spread to be known. From the last days of December 2020 and over 4 months, all respiratory samples with a positive result for SARS-CoV-2 from patients treated in primary care and the emergency department were screened to detect this variant based on the strategy S gene target failure (SGTF). The first cases were detected during week 53 (2020) and reached >90% of all cases during weeks 15–16 (2021). During this period, the B.1.1.7/SGTF variant spread at a rapid and constant replacement rate of around 30–36%. The probability of intensive care unit admission was twice higher among patients infected by the B.1.1.7/SGTF variant, but there were no differences in death rate. During the peak of the third pandemic wave, this variant was not the most prevalent, and it became dominant when this wave was declining. Our results confirm that the B.1.1.7/SGTF variant displaced other SARS-CoV-2 variants in our healthcare area in 4 months. This displacement has led to an increase in the burden of disease.
Tests of Strength Parameters of Hydro-Mixtures Based on Ashes from a Fluidized Bed Boiler in the In-Situ Approach
Authors: Popczyk Marcin
Published: July 2021

The paper presents the practical use of a solidifying hydro-mixture based on ashes from fluidized bed boilers under hard coal mine conditions for filling an incline connecting the headgate and tailgate of a longwall running along the strike with roof caving. The reason for filling the incline with a material of preset strength parameters was to minimize the methane hazard in the extracted coal seam. Due to a great demand for fill material, which translates into economic considerations, the option of applying fine-fraction waste material was selected. Preliminary laboratory tests of the physical and mechanical properties of hydro-mixtures based on ash obtained from a fluidized bed boiler of a power plant, allowed us to select a specific hydro-mixture meeting the requirements. After 95 days, the incline filled with the proposed hydro-mixture was subjected to exploitation along with the advance of longwall working. This enabled the in-situ collection of a number of fill material samples from various places along the entire length of the incline. Then their strength was tested and the results compared with the obtained test results of identical material seasoned under laboratory conditions. The obtained results constitute a unique research material since it is practically impossible to verify the laboratory-determined strength parameters of the solidifying fine-fraction hydro-mixtures under in-situ conditions. Thsis results mainly from the lack of technical capabilities and poor access to the places where fine-fraction hydro-mixtures are applied, mostly abandoned cavings or parts of workings separated by dams.
The Effect of pH, Ionic Strength and the Presence of PbII on the Formation of Calcium Carbonate from Homogenous Alkaline Solutions at Room Temperature
Authors: Di Lorenzo Fulvio , Steiner Kay , Churakov Sergey V.
Published: July 2021

Precipitation of calcium carbonates in aqueous systems is an important factor controlling various industrial, biological, and geological processes. In the first part of this study, the well-known titration approach introduced by Gebauer and coworkers in 2008 s used to obtain reliable experimental dataset for the deep understanding of CaCO3 nucleation kinetics in supersaturated solutions over a broad range of pH and ionic strength conditions. In the second part, the effect of impurities, i.e., 1 mol% of Pb2+, was assessed in the same range of experimental conditions. Divalent lead has been shown to have an inhibitory effect in all ranges of the conditions tested except for pH 8 and low ionic strength (≤0.15 mol/L). Future investigations might take advantage of the methodology and the data provided in this work to investigate the effect of other system variables. The investigation of all the major variables and the assessment of eventual synergic effects could improve our ability to predict the formation of CaCO3 in complex natural systems.
Worth from Waste: Utilizing a Graphite-Rich Fraction from Spent Lithium-Ion Batteries as Alternative Reductant in Nickel Slag Cleaning
Authors: Dańczak Anna , Ruismäki Ronja , Rinne Tommi , Klemettinen Lassi , O’Brien Hugh , Taskinen Pekka , Jokilaakso Ari , Serna-Guerrero Rodrigo
Published: July 2021

One possible way of recovering metals from spent lithium-ion batteries is to integrate the recycling with already existing metallurgical processes. This study continues our effort on integrating froth flotation and nickel-slag cleaning process for metal recovery from spent batteries (SBs), using anodic graphite as the main reductant. The SBs used in this study was a froth fraction from flotation of industrially prepared black mass. The effect of different ratios of Ni-slag to SBs on the time-dependent phase formation and metal behavior was investigated. The possible influence of graphite and sulfur contents in the system on the metal alloy/matte formation was described. The trace element (Co, Cu, Ni, and Mn) concentrations in the slag were analyzed using the laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) technique. The distribution coefficients of cobalt and nickel between the metallic or sulfidic phase (metal alloy/matte) and the coexisting slag increased with the increasing amount of SBs in the starting mixture. However, with the increasing concentrations of graphite in the starting mixture (from 0.99 wt.% to 3.97 wt.%), the Fe concentration in both metal alloy and matte also increased (from 29 wt.% to 68 wt.% and from 7 wt.% to 49 wt.%, respectively), which may be challenging if further hydrometallurgical treatment is expected. Therefore, the composition of metal alloy/matte must be adjusted depending on the further steps for metal recovery.
Quantification of Major Bioactive Constituents, Antioxidant Activity, and Enzyme Inhibitory Effects of Whole Coffee Cherries (Coffea arabica) and Their Extracts
Authors: Nemzer Boris , Kalita Diganta , Abshiru Nebiyu
Published: July 2021

Coffee cherry is a rich source of chlorogenic acids (CGAs) and caffeine. In this study we examined the potential antioxidant activity and enzyme inhibitory effects of whole coffee cherries (WCC) and their two extracts on α-amylase, α-glucosidase and acetylcholinesterase (AChE) activities, which are targets for the control of diabetes and Alzheimer’s diseases. Whole coffee cherry extract 40% (WCCE1) is rich in chlorogenic acid compounds, consisting of a minimum of 40% major isomers, namely 3-caffeoylquinic acids, 4-caffeoylquinic acids, 5-caffeoylquinic acids, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, 4-feruloylquinc acid, and 5-feruloylquinc acid. Whole coffee cherry extract 70% (WCCE2) is rich in caffeine, with a minimum of 70%. WCCE1 inhibited the activities of digestive enzymes α-amylase and α-glucosidase, and WCCE2 inhibited acetylcholinesterase activities with their IC50 values of 1.74, 2.42, and 0.09 mg/mL, respectively. Multiple antioxidant assays—including DPPH, ABTS, FRAP, ORAC, HORAC, NORAC, and SORAC—demonstrated that WCCE1 has strong antioxidant activity.
Is Curcumin the Answer to Future Chemotherapy Cocktail?
Authors: Kong Wei-Yang , Ngai Siew Ching , Goh Bey-Hing , Lee Learn-Han , Htar Thet-Thet , Chuah Lay-Hong
Published: July 2021

The rise in cancer cases in recent years is an alarming situation worldwide. Despite the tremendous research and invention of new cancer therapies, the clinical outcomes are not always reassuring. Cancer cells could develop several evasive mechanisms for their survivability and render therapeutic failure. The continuous use of conventional cancer therapies leads to chemoresistance, and a higher dose of treatment results in even greater toxicities among cancer patients. Therefore, the search for an alternative treatment modality is crucial to break this viscous cycle. This paper explores the suitability of curcumin combination treatment with other cancer therapies to curb cancer growth. We provide a critical insight to the mechanisms of action of curcumin, its role in combination therapy in various cancers, along with the molecular targets involved. Curcumin combination treatments were found to enhance anticancer effects, mediated by the multitargeting of several signalling pathways by curcumin and the co-administered cancer therapies. The preclinical and clinical evidence in curcumin combination therapy is critically analysed, and the future research direction of curcumin combination therapy is discussed.
A Review of Wine Authentication Using Spectroscopic Approaches in Combination with Chemometrics
Authors: Ranaweera Ranaweera K. R. , Capone Dimitra L. , Bastian Susan E. P. , Cozzolino Daniel , Jeffery David W.
Published: July 2021

In a global context where trading of wines involves considerable economic value, the requirement to guarantee wine authenticity can never be underestimated. With the ever-increasing advancements in analytical platforms, research into spectroscopic methods is thriving as they offer a powerful tool for rapid wine authentication. In particular, spectroscopic techniques have been identified as a user-friendly and economical alternative to traditional analyses involving more complex instrumentation that may not readily be deployable in an industry setting. Chemometrics plays an indispensable role in the interpretation and modelling of spectral data and is frequently used in conjunction with spectroscopy for sample classification. Considering the variety of available techniques under the banner of spectroscopy, this review aims to provide an update on the most popular spectroscopic approaches and chemometric data analysis procedures that are applicable to wine authentication.
Effect of Single and Dual Hydrothermal Treatments on the Resistant Starch Content and Physicochemical Properties of Lotus Rhizome Starches
Authors: Yeh Yun , Lai Lih-Shiuh
Published: July 2021

Heat-moisture treatment (HMT) changed the morphology and the degree of molecular ordering in lotus rhizome (Nelumbo nucifera Gaertn.) starch granules slightly, leading to some detectable cavities or holes near hilum, weaker birefringence and granule agglomeration, accompanied with modified XRD pattern from C- to A-type starch and lower relative crystallinity, particularly for high moisture HMT modification. In contrast, annealing (ANN) showed less impact on granule morphology, XRD pattern and relative crystallinity. All hydrothermal treatment decreased the resistant starch (from about 27.7–35.4% to 2.7–20%), increased the damage starch (from about 0.5–1.6% to 2.4–23.6%) and modified the functional and pasting properties of lotus rhizome starch pronouncedly. An increase in gelatinization temperature but a decrease in transition enthalpy occurred after hydrothermal modification, particularly for hydrothermal modification involved with HMT. HMT-modified starch also showed higher pasting temperature, less pronounced peak viscosity, leading to less significant thixotropic behavior and retrogradation during pasting-gelation process. However, single ANN treatment imparts a higher tendency of retrogradation as compared to native starch. For dual hydrothermally modified samples, the functional properties generally resembled to the behavior of single HMT-modified samples, indicating the pre- or post-ANN modification had less impact on the properties HMT modified lotus rhizome starch.
Design and Synthesis of New Acridone-Based Nitric Oxide Fluorescent Probe
Authors: Panfilov Mikhail , Chernova Darya , Khalfina Irina , Moskalensky Alexander , Vorob’ev Aleksey
Published: July 2021

Nitric oxide (NO) is an important signaling molecule involved in a wide range of physiological and pathological processes. Fluorescent imaging is a useful tool for monitoring NO concentration, which could be essential in various biological and biochemical studies. Here, we report the design of a novel small-molecule fluorescent probe based on 9(10H)acridone moiety for nitric oxide sensing. 7,8-Diamino-4-carboxy-10-methyl-9(10H)acridone reacts with NO in aqueous media in the presence of O2, yielding a corresponding triazole derivative with fivefold increased fluorescence intensity. The probe was shown to be capable of nitric oxide sensing in living Jurkat cells.
Engineering of Streptoalloteichus tenebrarius 2444 for Sustainable Production of Tobramycin
Authors: Mitousis Lena , Maier Hannes , Martinovic Luka , Kulik Andreas , Stockert Sigrid , Wohlleben Wolfgang , Stiefel Alfred , Musiol-Kroll Ewa
Published: July 2021

Tobramycin is a broad-spectrum aminoglycoside antibiotic agent. The compound is obtained from the base-catalyzed hydrolysis of carbamoyltobramycin (CTB), which is naturally produced by the actinomycete Streptoalloteichus tenebrarius. However, the strain uses the same precursors to synthesize several structurally related aminoglycosides. Consequently, the production yields of tobramycin are low, and the compound’s purification is very challenging, costly, and time-consuming. In this study, the production of the main undesired product, apramycin, in the industrial isolate Streptoalloteichus tenebrarius 2444 was decreased by applying the fermentation media M10 and M11, which contained high concentrations of starch and dextrin. Furthermore, the strain was genetically engineered by the inactivation of the aprK gene (∆aprK), resulting in the abolishment of apramycin biosynthesis. In the next step of strain development, an additional copy of the tobramycin biosynthetic gene cluster (BGC) was introduced into the ∆aprK mutant. Fermentation by the engineered strain (∆aprK_1-17L) in M11 medium resulted in a 3- to 4-fold higher production than fermentation by the precursor strain (∆aprK). The phenotypic stability of the mutant without selection pressure was validated. The use of the engineered S. tenebrarius 2444 facilitates a step-saving, efficient, and, thus, more sustainable production of the valuable compound tobramycin on an industrial scale.
Expanding the Use of Dynamic Electrostatic Repulsion Reversed-Phase Chromatography: An Effective Elution Mode for Peptides Control and Analysis
Authors: Mazzoccanti Giulia , Manetto Simone , Bassan Michele , Macis Marco , Iazzetti Antonia , Cabri Walter , Ricci Antonio , Gasparrini Francesco
Published: July 2021

Bioactive peptides are increasingly used in clinical practice. Reversed-phase chromatography using formic or trifluoroacetic acid in the mobile phase is the most widely used technique for their analytical control. However, sometimes it does not prove sufficient to solve challenging chromatographic problems. In the search for alternative elution modes, the dynamic electrostatic repulsion reversed-phase was evaluated to separate eight probe peptides characterised by different molecular weights and isoelectric points. This technique, which involves TBAHSO4 in the mobile phase, provided the lowest asymmetry and peak width at half height values and the highest in peak capacity (about 200 for a gradient of 30 min) and resolution concerning the classic reversed-phase. All analyses were performed using cutting-edge columns developed for peptide separation, and the comparison of the chromatograms obtained shows how the dynamic electrostatic repulsion reversed-phase is an attractive alternative to the classic reversed-phase.
A Hierarchically Ordered Mesoporous-Carbon-Supported Iron Sulfide Anode for High-Rate Na-Ion Storage
Authors: Haridas Anupriya K. , Angulakshmi Natarajan , Stephan Arul Manuel , Lee Younki , Ahn Jou-Hyeon
Published: July 2021

Sodium-ion batteries (SIBs) are promising alternatives to lithium-based energy storage devices for large-scale applications, but conventional lithium-ion battery anode materials do not provide adequate reversible Na-ion storage. In contrast, conversion-based transition metal sulfides have high theoretical capacities and are suitable anode materials for SIBs. Iron sulfide (FeS) is environmentally benign and inexpensive but suffers from low conductivity and sluggish Na-ion diffusion kinetics. In addition, significant volume changes during the sodiation of FeS destroy the electrode structure and shorten the cycle life. Herein, we report the rational design of the FeS/carbon composite, specifically FeS encapsulated within a hierarchically ordered mesoporous carbon prepared via nanocasting using a SBA-15 template with stable cycle life. We evaluated the Na-ion storage properties and found that the parallel 2D mesoporous channels in the resultant FeS/carbon composite enhanced the conductivity, buffered the volume changes, and prevented unwanted side reactions. Further, high-rate Na-ion storage (363.4 mAh g−1 after 500 cycles at 2 A g−1, 132.5 mAh g−1 at 20 A g−1) was achieved, better than that of the bare FeS electrode, indicating the benefit of structural confinement for rapid ion transfer, and demonstrating the excellent electrochemical performance of this anode material at high rates.
Oxyresveratrol-Loaded PLGA Nanoparticles Inhibit Oxygen Free Radical Production by Human Monocytes: Role in Nanoparticle Biocompatibility
Authors: Donini Marta , Gaglio Salvatore Calogero , Laudanna Carlo , Perduca Massimiliano , Dusi Stefano
Published: July 2021

Oxyresveratrol, a polyphenol extracted from the plant Artocarpus lakoocha Roxb, has been reported to be an antioxidant and an oxygen-free radical scavenger. We investigated whether oxyresveratrol affects the generation of superoxide anion (O2−) by human monocytes, which are powerful reactive oxygen species (ROS) producers. We found that oxyresveratrol inhibited the O2− production induced upon stimulation of monocytes with β-glucan, a well known fungal immune cell activator. We then investigated whether the inclusion of oxyresveratrol into nanoparticles could modulate its effects on O2− release. We synthesized poly(lactic-co-glycolic acid) (PLGA) nanoparticles, and we assessed their effects on monocytes. We found that empty PLGA nanoparticles induced O2− production by resting monocytes and enhanced the formation of this radical in β-glucan-stimulated monocytes. Interestingly, the insertion of oxyresveratrol into PLGA nanoparticles significantly inhibited the O2− production elicited by unloaded nanoparticles in resting monocytes as well as the synergistic effect of nanoparticles and β-glucan. Our results indicate that oxyresveratrol is able to inhibit ROS production by activated monocytes, and its inclusion into PLGA nanoparticles mitigates the oxidative effects due to the interaction between these nanoparticles and resting monocytes. Moreover, oxyresveratrol can contrast the synergistic effects of nanoparticles with fungal agents that could be present in the patient tissues. Therefore, oxyresveratrol is a natural compound able to make PLGA nanoparticles more biocompatible.
BookTubers as Multimodal Reading Influencers: An Analysis of Subscriber Interactions
Authors: Roig-Vila Rosabel , Romero-Guerra Héctor , Rovira-Collado José
Published: July 2021

The objective of the study was to learn about the relationships between BookTubers and their subscribers by focusing on the comments left by viewers of the audio-visual literary reviews. We also examined whether viewer-BookTuber relationships resulted in the promotion of reading. A mixed qualitative-quantitative methodology was followed, including a descriptive analysis of contents and a case study. The main tools used were MAXQDA to process the qualitative data and Excel to obtain the quantitative data. The sample was a non-random selection of four BookTubers channels, taking into account both their impact and gender equality (two female and two male BookTubers). The categorization was conducted based on Cultural Studies and Reception Aesthetics. A total of eight videos (four reviews and four Book Hauls) were selected and 100 comments on each were analyzed, giving rise to four categories. The results indicated that in terms of content decoding, close relationships were established among community members, between both consumers and producers. In addition, message acceptance took place and a certain relationship was found between the BookTuber’s work and the promotion of reading. BookTubers were therefore identified as multimodal influencers.
Tunable Transmissive Terahertz Linear Polarizer for Arbitrary Linear Incidence Based on Low-Dimensional Metamaterials
Authors: Yang Zhenyu , Yu Dahai , Zhang Huiping , Yu Anqi , Guo Xuguang , Ren Yuxiang , Zang Xiaofei , Balakin Alexei V. , Shkurinov Alexander P.
Published: July 2021

In this work, we propose a structure consisting of three metamaterial layers and a metallic grating layer to rotate the polarization of arbitrary linearly polarized incidence to the y-direction with high transmissivity by electrically tuning these metamaterials. The transfer matrix method together with a harmonic oscillator model is adopted to theoretically study the proposed structure. Numerical simulation based on the finite difference time-domain method is performed assuming that the metamaterial layers are constituted by graphene ribbon arrays. The calculation and simulation results show that the Drude absorption is responsible for the polarization rotation. Fermi level and scattering rate of graphene are important for the transmissivity. For a polarization rotation of around 90°, the thickness of either the upper or lower dielectric separations influences the transmission window. For a polarization rotation of around 45° and 135°, the lower dielectric separations decide the frequency of the transmission window, while the upper dielectric separations just slightly influence the transmissivity.
Characterization of Oral Microbiota in Cats: Novel Insights on the Potential Role of Fungi in Feline Chronic Gingivostomatitis
Authors: Krumbeck Janina A. , Reiter Alexander M. , Pohl James C. , Tang Shuiquan , Kim Young J. , Linde Annika , Prem Aishani , Melgarejo Tonatiuh
Published: July 2021

Previous studies have suggested the involvement of viral and bacterial components in the initiation and progression of feline chronic gingivostomatitis (FCGS), but the role of fungi remains entirely unknown. This pilot study aimed to investigate the bacteriome and mycobiome in feline oral health and disease. Physical exams, including oral health assessment, of privately owned, clinically healthy (CH) cats (n = 14) and cats affected by FCGS (n = 14) were performed. Using a sterile swab, oral tissue surfaces of CH and FCGS cats were sampled and submitted for 16S rRNA and ITS-2 next-generation DNA sequencing. A high number of fungal species (n = 186) was detected, with Malassezia restricta, Malassezia arunalokei, Cladosporium penidielloides/salinae, and Aspergillaceae sp. being significantly enriched in FCGS samples, and Saccharomyces cerevisiae in CH samples. The bacteriome was significantly distinct between groups, and significant inter-kingdom interactions were documented. Bergeyella zoohelcum was identified as a potential biomarker of a healthy feline oral microbiome. These data suggest that fungi might play a role in the etiology and pathogenesis of FCGS, and that oral health should not simply be regarded as the absence of microbial infections. Instead, it may be viewed as the biological interactions between bacterial and fungal populations that coexist to preserve a complex equilibrium in the microenvironment of the mouth. Additional investigations are needed to improve our understanding of the feline oral ecosystem and the potential interactions between viruses, bacteria, and fungi in FCGS.
The Role of Macrophages in the Host’s Defense against Sporothrix schenckii
Authors: Ruiz-Baca Estela , Pérez-Torres Armando , Romo-Lozano Yolanda , Cervantes-García Daniel , Alba-Fierro Carlos A. , Ventura-Juárez Javier , Torriello Conchita
Published: July 2021

The role of immune cells associated with sporotrichosis caused by Sporothrix schenckii is not yet fully clarified. Macrophages through pattern recognition receptors (PRRs) can recognize pathogen-associated molecular patterns (PAMPs) of Sporothrix, engulf it, activate respiratory burst, and secrete pro-inflammatory or anti-inflammatory biological mediators to control infection. It is important to consider that the characteristics associated with S. schenckii and/or the host may influence macrophage polarization (M1/M2), cell recruitment, and the type of immune response (1, 2, and 17). Currently, with the use of new monocyte-macrophage cell lines, it is possible to evaluate different host–pathogen interaction processes, which allows for the proposal of new mechanisms in human sporotrichosis. Therefore, in order to contribute to the understanding of these host–pathogen interactions, the aim of this review is to summarize and discuss the immune responses induced by macrophage-S. schenckii interactions, as well as the PRRs and PAMPs involved during the recognition of S. schenckii that favor the immune evasion by the fungus.
Natural Infection of Dairy Cows with Bovine Leukemia Virus Affects Immunoglobulin Levels in Saliva and Serum but Not Milk
Authors: Dziuba Monika , Ruggiero Vickie J. , Wilson Catherine , Bartlett Paul C. , Coussens Paul M.
Published: July 2021

Bovine leukemia virus (BLV) is a retroviral infection that disrupts the immune function of infected animals. It is widespread among U.S. dairy cattle. In this pilot study, the average total IgA and IgM concentrations in milk, saliva, and serum samples from BLV ELISA-positive (ELISA+) dairy cows were compared against samples from BLV ELISA-negative (ELISA−) cows using the Kruskal–Wallis test (with ties). The results from ELISA+ cows were also stratified by lymphocyte count (LC) and proviral load (PVL). In milk and saliva from ELISA+ cows, the average total IgA and IgM concentrations were decreased compared to ELISA− cows, although this was only statistically significant for saliva IgM in cows with low PVL (p = 0.0424). Numerically, the average total IgA concentrations were 33.6% lower in milk and 23.7% lower in saliva, and the average total IgM concentrations were 42.4% lower in milk and 15.5% lower in saliva. No significant differences were observed in the total serum IgA concentrations, regardless of PVL and LC. The total serum IgM from ELISA+ cows was significantly decreased (p = 0.0223), with the largest decreases occurring in the highest PVL and LC subgroups. This pilot study is a first step in investigating the impact of BLV on mucosal immunity and will require further exploration in each of the various stages of disease progression.
Intranasal Administration for Pain: Oxytocin and Other Polypeptides
Authors: Bharadwaj Vimala N. , Tzabazis Alexander Z. , Klukinov Michael , Manering Neil A. , Yeomans David C.
Published: July 2021

Pain, particularly chronic pain, remains one of the most debilitating and difficult-to-treat conditions in medicine. Chronic pain is difficult to treat, in part because it is associated with plastic changes in the peripheral and central nervous systems. Polypeptides are linear organic polymers that are highly selective molecules for neurotransmitter and other nervous system receptors sites, including those associated with pain and analgesia, and so have tremendous potential in pain therapeutics. However, delivery of polypeptides to the nervous system is largely limited due to rapid degradation within the peripheral circulation as well as the blood–brain barrier. One strategy that has been shown to be successful in nervous system deposition of polypeptides is intranasal (IN) delivery. In this narrative review, we discuss the delivery of polypeptides to the peripheral and central nervous systems following IN administration. We briefly discuss the mechanism of delivery via the nasal–cerebral pathway. We review recent studies that demonstrate that polypeptides such as oxytocin, delivered IN, not only reach key pain-modulating regions in the nervous system but, in doing so, evoke significant analgesic effects. IN administration of polypeptides has tremendous potential to provide a non-invasive, rapid and effective method of delivery to the nervous system for chronic pain treatment and management.
On the Explicit Function of Life within a Physical Universe
Authors: Grathoff Annette
Published: July 2021

To describe the meaning of functionality in a universe before life evolved, existing etiological and systemic accounts of function are evaluated. Since the theory of function is only applicable in context with living beings and artifacts used by living beings and therefore cannot predict how a prebiotic form of functionality could evolve, a maintenance account for functionality is proposed. This account ascribes functionality to a structurally disposed property that increases the probability of maintenance or recurrence of the property in the surrounding selective environment. With the help of the maintenance account and a concept of physical information comprising kinetic and structural types of information, possible evolutionary processes preceding the evolution of life are explored. As important mechanisms in abiotic and prebiotic evolution, linear and non-linear mixing processes, as well as dynamics of solitary waves, are identified. Before the question of the meaning of life in prebiotic environments is renewed and an educated guess based on the elaborated arguments is made on the progress of evolution under the influencing impression of the living state, the evolution of functionality in different selective contexts is analyzed.
Downregulation of Salivary Proteins, Protective against Dental Caries, in Type 1 Diabetes
Authors: Pappa Eftychia , Vougas Konstantinos , Zoidakis Jerome , Papaioannou William , Rahiotis Christos , Vastardis Heleni
Published: July 2021

Saliva, an essential oral secretion involved in protecting the oral cavity’s hard and soft tissues, is readily available and straightforward to collect. Recent studies have analyzed the salivary proteome in children and adolescents with extensive carious lesions to identify diagnostic and prognostic biomarkers. The current study aimed to investigate saliva’s diagnostic ability through proteomics to detect the potential differential expression of proteins specific for the occurrence of carious lesions. For this study, we performed bioinformatics and functional analysis of proteomic datasets, previously examined by our group, from samples of adolescents with regulated and unregulated type 1 diabetes, as they compare with healthy controls. Among the differentially expressed proteins relevant to caries pathology, alpha-amylase 2B, beta-defensin 4A, BPI fold containing family B member 2, protein S100-A7, mucin 5B, statherin, salivary proline-rich protein 2, and interleukin 36 gamma were significantly downregulated in poorly-controlled patients compared to healthy subjects. In addition, significant biological pathways (defense response to the bacterium, beta-defensin activity, proline-rich protein activity, oxygen binding, calcium binding, and glycosylation) were deregulated in this comparison, highlighting specific molecular characteristics in the cariogenic process. This analysis contributes to a better understanding of the mechanisms involved in caries vulnerability in adolescents with unregulated diabetes.
Spiritual and Religious Support for Underrepresented First-Generation, Low-Income (UFGLI) Students
Authors: Ingersoll Elliott , Elliott Sophia , Drcar Stephanie
Published: July 2021

UFGLI students comprise 34% of the students enrolled in four-year universities. Unlike some students, UFGLI students face internal and systemic barriers throughout their educational experience and their struggles are often dismissed and disregarded. Working and raising a family while taking courses, minimal support systems, and financial struggles require students to optimize their resources. We explore the issues of UFGLI students and the importance of their spiritual and religious supports using a literature review and a case study. Religious and spiritual identities are resources that should be explored and supported by staff at university counselling centers. Affirming UFGLI students’ religious and spiritual identities and understanding how religion and spirituality work in their lives can assist these students in their acclimation to and success at university.
Refocusing of Moving Ships in Squint SAR Images Based on Spectrum Orthogonalization
Authors: Tong Xuyao , Bao Min , Sun Guangcai , Han Liang , Zhang Yu , Xing Mengdao
Published: July 2021

Moving ship refocusing is challenging because the target motion parameters are unknown. Moreover, moving ships in squint synthetic aperture radar (SAR) images obtained by the back-projection (BP) algorithm usually suffer from geometric deformation and spectrum winding. Therefore, a spectrum-orthogonalization algorithm that refocuses moving ships in squint SAR images is presented. First, “squint minimization” is introduced to correct the spectrum by two spectrum compression functions: one to align the spectrum centers and another to translate the inclined spectrum into orthogonalized form. Then, the precise analytic function of the two-dimensional (2D) wavenumber spectrum is derived to obtain the phase error. Finally, motion compensation is performed in the two-dimensional wavenumber domain after the motion parameter is estimated by maximizing the image sharpness. This method has low computational complexity because it lacks interpolation and can be implemented by the inverse fast Fourier translation (IFFT) and fast Fourier translation (FFT). Processing results of simulation experiments and the GaoFen-3 squint SAR data validate the effectiveness of this method.
Seismic Ambient Noise Imaging of a Quasi-Amagmatic Ultra-Slow Spreading Ridge
Authors: Mohamadian Sarvandani Mohamadhasan , Kästle Emanuel , Boschi Lapo , Leroy Sylvie , Cannat Mathilde
Published: July 2021

Passive seismic interferometry has become very popular in recent years in exploration geophysics. However, it has not been widely applied in marine exploration. The purpose of this study is to investigate the internal structure of a quasi-amagmatic portion of the Southwest Indian Ridge by interferometry and to examine the performance and reliability of interferometry in marine explorations. To reach this goal, continuous vertical component recordings from 43 ocean bottom seismometers were analyzed. The recorded signals from 200 station pairs were cross-correlated in the frequency domain. The Bessel function method was applied to extract phase–velocity dispersion curves from the zero crossings of the cross-correlations. An average of all the dispersion curves was estimated in a period band 1–10 s and inverted through a conditional neighborhood algorithm which led to the final 1D S-wave velocity model of the crust and upper mantle. The obtained S-wave velocity model is in good agreement with previous geological and geophysical studies in the region and also in similar areas. We find an average crustal thickness of 7 km with a shallow layer of low shear velocities and high Vp/Vs ratio. We infer that the uppermost 2 km are highly porous and may be strongly serpentinized.
Inverse Synthetic Aperture Radar Sparse Imaging Exploiting the Group Dictionary Learning
Authors: Hu Changyu , Wang Ling , Zhu Daiyin , Loffeld Otmar
Published: July 2021

Sparse imaging relies on sparse representations of the target scenes to be imaged. Predefined dictionaries have long been used to transform radar target scenes into sparse domains, but the performance is limited by the artificially designed or existing transforms, e.g., Fourier transform and wavelet transform, which are not optimal for the target scenes to be sparsified. The dictionary learning (DL) technique has been exploited to obtain sparse transforms optimized jointly with the radar imaging problem. Nevertheless, the DL technique is usually implemented in a manner of patch processing, which ignores the relationship between patches, leading to the omission of some feature information during the learning of the sparse transforms. To capture the feature information of the target scenes more accurately, we adopt image patch group (IPG) instead of patch in DL. The IPG is constructed by the patches with similar structures. DL is performed with respect to each IPG, which is termed as group dictionary learning (GDL). The group oriented sparse representation (GOSR) and target image reconstruction are then jointly optimized by solving a l1 norm minimization problem exploiting GOSR, during which a generalized Gaussian distribution hypothesis of radar image reconstruction error is introduced to make the imaging problem tractable. The imaging results using the real ISAR data show that the GDL-based imaging method outperforms the original DL-based imaging method in both imaging quality and computational speed.