bluuebunny/crossref_metadata_2025 huggingface dataset:
A first-principles study on the effect of Cr, Mn, and Co substitution on Fe-based normal- and inverse-Heusler compounds: Fe3−xYxZ (x=0, 1, 2, 3; Y= Cr, Mn, Co; Z=Al, Ga, Si)
Authors: Huang Hung-Lung , Tung Jen-Chuan , Jeng Horng-Tay
Published: September 2022

First-principles calculation has become one of the most reliable approaches in predicting structural, electronic, and magnetic properties for material applications. Alloys in Heusler structures have also attracted much attention recently since they can be easily synthesized and provide interesting properties for future spintronic applications. In this work, we investigate a series of Fe-based Heusler compounds Fe3−xYxZ (x = 0, 1, 2, 3; Y= Cr, Mn, Co; Z= Al, Ga, Si) with L21- and XA-type structures using first-principles calculations based on density functional theory. According to formation energy calculations and mechanical property analysis, most of the studied Heusler compounds are thermodynamically stable and could be synthesized experimentally. The Co substitution leads Fe3−xCoxZ to a ferromagnetic ground state like Fe3Z with a strong magnetization ranging from 4 to 6 μB/f. u. While replacing Fe with Cr or Mn, the exchange coupling between Cr (Mn) and its neighboring atoms generally tend to be anti-parallel. Among the antiferromagnetic compounds, Mn3Al and Mn3Ga are antiferromagnetic half metal while Mn3Si is ferrimagnetic half metal. These rarely found type of half metals with low magnetic moment and high spin polarization at the Fermi level are important for low energy consumption spintronic applications. The estimated Curie temperatures for Mn3Al, and Mn3Si and Co2FeSi (XA) are in good agreement with previously theoretical values, while for Fe3Al and Fe3Si, they are in good agreement with previous experimental results. The good consistency in Curie temperature demonstrates high reliability of our predictions based on first-principles calculations. As for the topological property aspect, we predict Fe2CrAl and Fe2MnAl as the 3-dimensional Weyl semimetal. Furthermore, Fe2CrSi is predicted to be the magnetic nodal-line semimetal. Interestingly, our mechanical property analysis demonstrates that Co3Si and Fe2CoSi (L21) exhibit ultraelastic metal behavior, which is of high potential in advanced mechanical industry. This work suggests that Heusler compounds are excellent candidates for future spintronics as well as for high-performance ultraelastic metals.
Diagnostic values of 2 different techniques for controversial lumbar disc herniation by conventional imaging examination: 3D-DESS vs. CT plain scan
Authors: Liu Wei , Chen Jinhua , Zhang Yanan , Wang Xu , Zheng Junwen , Huang Aibing , Chen Chunmao , Bian Jian , Yang Lei , Li Haijun
Published: September 2022

Background: The aim of this study was to explore the significance of three-dimensional double-echo steady-state (3D-DESS) sequence and multidetector computed tomography (CT) plain scan in the diagnosis of lumbar disc herniation (LDH) remaining controversial in conventional magnetic resonance imaging (MRI), and to compare the efficiency between 3D-DESS and CT in diagnosing controversial patients by conventional MRI.

Methods: A total of 61 patients with controversial LDH diagnosed by conventional MRI were collected. Before operation, the disease of these patients was further confirmed by 3D-DESS sequences and continuous CT plain scan from L3 to S1. Finally, for patients whose postoperative curative effect was marked and symptoms were obviously alleviated, the sensitivity, specificity and accuracy.

Results: Among, 59 patients with remarkably relieved symptoms after operation were included, and 2 patients with varying degrees of non-remission of pain and partial dysfunction after operation were excluded. The sensitivity, specificity and accuracy of 3D-DESS were 94.6, 100 and 94.9%, respectively, and those of CT were 75.0, 33.3 and 72.9%, respectively.

Conclusion: 3D-DESS is a very useful diagnostic method for patients with some special types of LDH that remain controversial in conventional imaging diagnostic methods. Through 3D-DESS, the morphology of lumbosacral nerve roots can be directly observed, which is conducive to the improvement of the sensitivity, specificity and accuracy, thus further reducing the misdiagnosis rate. Moreover, 3D-DESS plays a guiding role in the formulation of operative methods.
Tomato salt tolerance mechanisms and their potential applications for fighting salinity: A review
Authors: Guo Meng , Wang Xin-Sheng , Guo Hui-Dan , Bai Sheng-Yi , Khan Abid , Wang Xiao-Min , Gao Yan-Ming , Li Jian-She
Published: September 2022

One of the most significant environmental factors affecting plant growth, development and productivity is salt stress. The damage caused by salt to plants mainly includes ionic, osmotic and secondary stresses, while the plants adapt to salt stress through multiple biochemical and molecular pathways. Tomato (Solanum lycopersicum L.) is one of the most widely cultivated vegetable crops and a model dicot plant. It is moderately sensitive to salinity throughout the period of growth and development. Biotechnological efforts to improve tomato salt tolerance hinge on a synthesized understanding of the mechanisms underlying salinity tolerance. This review provides a comprehensive review of major advances on the mechanisms controlling salt tolerance of tomato in terms of sensing and signaling, adaptive responses, and epigenetic regulation. Additionally, we discussed the potential application of these mechanisms in improving salt tolerance of tomato, including genetic engineering, marker-assisted selection, and eco-sustainable approaches.
Vitamin D status in children with a psychiatric diagnosis, autism spectrum disorders, or internalizing disorders
Authors: Muskens Jet , Klip Helen , Zinkstok Janneke R. , van Dongen-Boomsma Martine , Staal Wouter G.
Published: September 2022

Background and objective

Multiple studies suggest that children with Autism Spectrum Disorders (ASD) have significantly lower vitamin D3 levels than typically developing children. However, whether vitamin D3 deficiency is more common in children with ASD than in children with other psychiatric disorders remains unclear. This study was conducted to explore the prevalence of vitamin D3 in children with a psychiatric diagnosis including children with ASD or with internalizing disorders (mood and anxiety disorders). In addition, this study investigated the potential associations between vitamin D3 and Body Mass Index (BMI).

Materials and methods

Clinical data, including BMI and vitamin D3 levels, of 93 children (6–18 years; n = 47; 51% female) with ASD (n = 58) and internalizing disorders (n = 37) were retrospectively analyzed.

Results

In the overall sample, the prevalence of vitamin D3 deficiency (<50 nmol/L) was 77.4%. Additionally, 75.9% of the children with ASD and 79.5% with internalizing disorders had vitamin D3 deficiency. BMI was inversely related to vitamin D3 in the total group (p = 0.016). The multiple regression model for the total group significantly predicted vitamin D3 (p = 0.022). Age contributed significantly to the prediction. Stratified for sex and primary diagnosis, multiple regression models showed that for boys with ASD, higher BMI levels were associated with lower vitamin D3 levels (p = 0.031); in boys with internalizing disorders and in girls, no relation was found between BMI and vitamin D3 levels.

Conclusion

In this this cross-sectional, explorative study high rates of vitamin D3 deficiency in children with different psychiatric disorders were found. The results showed an inverse relation between BMI and vitamin D3 levels in the total group. Vitamin D3 deficiency was particularly common in boys with ASD and obesity. Lifestyle factors may contribute to the association between high BMI and low vitamin D3 levels in boys with ASD. Vitamin D3 deficiency is common in patients with psychiatric disorders and it is highly recommended to increase clinicians’ awareness of this common and remediable risk factor.
The mediation and interaction of depressive symptoms in activities of daily living and active aging in rural elderly: A cross-sectional survey
Authors: Fu Xuelian , Su Yinli , Zeng Chunyan , Liu Liqiong , Guo Yang , Wu Yuanyuan
Published: September 2022

Background

Compared with urban areas, old adults in rural areas have limited access to medical and health resources in China. Active of daily living ability (ADL) decline and depressive symptoms are common in rural older adults. In particular, the depressive symptoms of the elderly in rural areas are often ignored. Thus, it is difficult to realize high-level active aging at the individual level. In order to explore the effects of ADL and depressive symptoms on the active aging of rural elderly, we conducted a survey and analyzed the mediation and interaction effects of depressive symptoms of ADL on active aging.

Methods

From July to November 2019, a cross-sectional study of 945 elderly rural individuals was conducted in three townships in Xiangtan County, China. Active aging, ADL, and depressive symptoms were assessed using the positive aging questionnaire (PAEQ), ADL scale, and depression in old age scale (DIA-S), respectively. PROCESS macro program model 4 and logistic regression were used to explore the mediation and interaction between ADL and depressive symptoms on active aging.

Results

The proportions of rural elderly with an active aging level were 23.5% (well above average), 50.9% (above average), 24.1% (below average), 1.5% (well below average), respectively. The rates of ADL decline and depressive symptoms were 44.7 and 19.7%, respectively. Mediated effect analysis showed that the relationship between ADL and active aging could be partly mediated by depressive symptoms (ab = −0.2382, boot SE = 0.0437), and the 95% confidence interval was [−0.3311, −0.1584]. The mediating effect proportion of the total effect was 30.7%. Logistic regression showed that ADL and depressive symptoms have an interactive additive effect on active aging. The relative excess risk of interaction (RERI), the attributable proportion due to interaction (API), and the synergy index (SI) scores were 13.109, 0.621, and 2.871, respectively. Older adults with ADL decline and depressive symptoms had higher (OR = 21.115) odds of well-below-average active aging compared with older adults with ADL decline (OR = 3.258) or only depressive symptoms (OR = 5.749).

Conclusion

The findings suggest that the association between ADL and active aging is persistent and partly mediated by depressive symptoms, and comorbid depressive symptoms and ADL decline have an additive effect on active aging. Maintaining independence is an important factor for realizing active aging. However, for the rural elderly with ADL decline and low-level active aging, we can promote the realization of high-level active aging at the individual level through the prevention and treatment of depressive symptoms based on multidisciplinary care.
Burnout, negative emotions, and wellbeing among social workers in China after community lockdowns during the COVID-19 pandemic: Mediating roles of trait mindfulness
Authors: Wu Yaxue , Wei Yue , Li Yanli , Pang Jun , Su Yang
Published: September 2022

Objective

This study aimed to investigate burnout situation of social workers (SWs) who experienced the COVID-19 pandemic-related community lockdown 1 year before, and to assess the protective value of trait mindfulness (TM) in states of burnout.

Method

We surveyed the burnout, trait mindfulness, negative emotions (NEs) and wellbeing (WB) of 182 social workers provided services to Wuhan lockdowns community by COVID-19 one year before. Burnout were measured using the Maslach Burnout Inventory–Human Services Survey; TM using the Mindful Attention Awareness Scale; NEs using the Depression Anxiety and Stress Scale-21; and WB using the General Wellbeing Schedule. We also performed correlation regression analysis and mediation test for burnout, TM, NEs, and WB.

Results

Among the 182 respondents, 75 (41.2%) still suffered from severe burnout. TM was negatively correlated with burnout (r = −0.623), negatively correlated with NEs (r = −0.560), and positively correlated with WB (r = 0.617). Burnout had a significantly positive correlation with NEs (r = 0.544) and a significantly negative correlation with WB (r = −0.666). Further, WB had significantly negative correlation with NEs (r = −0.758). After controlling for age, gender, marital status, educational level, and years of employment, burnout had a significantly positive predictive effect on NEs (β = 0.509), whereas TM had a significantly negative predictive effect on NEs (β = −0.334). TM played a partial mediating role in the effect of burnout on NEs, with a mediating effect and effect ratio of 0.088 and 39.7%, respectively. Burnout had a significantly negative predictive effect on WB (β = −0.598), whereas TM had a significantly positive predictive effect on WB (β = 0.299). TM played a partial mediating role in the effect of burnout on NEs, with a mediating effect and effect ratio of −0.164 and 30.3%, respectively. WB had a significantly negative predictive effect on NEs (β = −0.711), and it played a partial mediating role in the effect of burnout on NEs, with a mediating effect and effect ratio of 0.185 and 83.3%, respectively.

Conclusion

The current levels of burnout among local SWs remained high 1 year after the community lockdowns. TM played a mediating role in the relationship between burnout, NEs, and WB. Concomitantly, WB played a mediating role in the relationship between burnout and NEs. Therefore, in the context of burnout, TM is a protective factor for reducing emotional stress and risks of developing psychiatric disorders through the enhancement of WB.
Generating high-fidelity privacy-conscious synthetic patient data for causal effect estimation with multiple treatments
Authors: Shi Jingpu , Wang Dong , Tesei Gino , Norgeot Beau
Published: September 2022

In the past decade, there has been exponentially growing interest in the use of observational data collected as a part of routine healthcare practice to determine the effect of a treatment with causal inference models. Validation of these models, however, has been a challenge because the ground truth is unknown: only one treatment-outcome pair for each person can be observed. There have been multiple efforts to fill this void using synthetic data where the ground truth can be generated. However, to date, these datasets have been severely limited in their utility either by being modeled after small non-representative patient populations, being dissimilar to real target populations, or only providing known effects for two cohorts (treated vs. control). In this work, we produced a large-scale and realistic synthetic dataset that provides ground truth effects for over 10 hypertension treatments on blood pressure outcomes. The synthetic dataset was created by modeling a nationwide cohort of more than 580, 000 hypertension patient data including each person's multi-year history of diagnoses, medications, and laboratory values. We designed a data generation process by combining an adapted ADS-GAN model for fictitious patient information generation and a neural network for treatment outcome generation. Wasserstein distance of 0.35 demonstrates that our synthetic data follows a nearly identical joint distribution to the patient cohort used to generate the data. Patient privacy was a primary concern for this study; the ϵ-identifiability metric, which estimates the probability of actual patients being identified, is 0.008%, ensuring that our synthetic data cannot be used to identify any actual patients. To demonstrate its usage, we tested the bias in causal effect estimation of four well-established models using this dataset. The approach we used can be readily extended to other types of diseases in the clinical domain, and to datasets in other domains as well.
“Provider discretionary power practices to support implementation of patient-centered HIV care in Lusaka, Zambia”
Authors: Mwamba Chanda , Mukamba Njekwa , Sharma Anjali , Lumbo Kasapo , Foloko Marksman , Nyirenda Herbert , Simbeza Sandra , Sikombe Kombatende , Holmes Charles B. , Sikazwe Izukanji , Moore Carolyn Bolton , Mody Aaloke , Geng Elvin , Beres Laura K.
Published: September 2022

Introduction

Traditional patient-provider relationships privilege the providers, as they possess the formal authority and clinical knowledge applied to address illness, but providers also have discretion over how they exercise their power to influence patients' services, benefits, and sanctions. In this study, we assessed providers' exercise of discretionary power in implementing patient-centered care (PCC) practices in Lusaka, Zambia.

Methods

HIV clinical encounters between patients on antiretroviral therapy (ART) and providers across 24 public health facilities in Lusaka Province were audio recorded and transcribed verbatim. Using qualitative content analysis, we identified practices of discretionary power (DP) employed in the implementation of PCC and instances of withholding DP. A codebook of DP practices was inductively and iteratively developed. We compared outcomes across provider cadres and within sites over time.

Results

We captured 194 patient-provider interactions at 24 study sites involving 11 Medical Officers, 58 Clinical Officers and 10 Nurses between August 2019 to May 2021. Median interaction length was 7.5 min. In a hierarchy where providers dominate patients and interactions are rapid, some providers invited patients to ask questions and responded at length with information that could increase patient understanding and agency. Others used inclusive language, welcomed patients, conducted introductions, and apologized for delayed services, narrowing the hierarchical distance between patient and provider, and facilitating recognition of the patient as a partner in care. Although less common, providers shared their decision-making powers, allowing patients to choose appointment dates and influence regimens. They also facilitated resource access, including access to services and providers outside of scheduled appointment times. Application of DP was not universal and missed opportunities were identified.

Conclusion

Supporting providers to recognize their power and intentionally share it is both inherent to the practice of PCC (e.g., making a patient a partner), and a way to implement improved patient support. More research is needed to understand the application of DP practices in improving the patient-centeredness of care in non-ART settings.
The impact of non-modifiable sociodemographic factors on bladder cancer survival outcomes after radical cystectomy: A systematic review and cumulative analysis of population cohort studies
Authors: Yip Wesley , Chen Andrew B. , Basin Michael F. , Cacciamani Giovanni E. , Bhanvadia Sumeet K.
Published: September 2022

Introduction

Sociodemographic factors have been shown to have significant impacts on bladder cancer (BC) outcomes, but there are conflicting data in the literature regarding certain non-modifiable factors. We sought to determine the effect of sociodemographic factors on survival outcomes after radical cystectomy (RC) for BC.

Materials and methods

A systematic review of population-based cohort studies published before March 2020 from Surveillance, Epidemiology, and End Results (SEER) and National Cancer Database (NCDB) was performed per Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines by searching PubMed®, Scopus®, and Web of Science®. All full-text English-language articles assessing the impact of sociodemographic factors on BC survival after RC were obtained. Two investigators (WY and AC) independently screened all articles. Discrepancies were resolved by consensus. All studies reporting survival outcomes after RC based on any of the sociodemographic factors were included, except for systematic reviews, which were excluded. Primary end points were overall survival (OS) and disease-specific survival (DSS) after RC. Cohort studies reporting Cox proportional hazards or logistic regression analysis were independently screened. Available multivariable hazard ratios (HRs) were included in the quantitative analysis.

Results

Our search returned 147 studies, of which 14 studies (11 SEER and 3 NCDB) were included for cumulative analysis. Only race and gender were evaluable due to heterogeneity of other factors. Compared to White patients, Black patients have worse OS [HR 0.83; 95% confidence intervals (CIs) 0.75, 0.92; p < 0.01; I2 = 79%] and DSS (HR 0.83; 95% CI 0.69, 1.00; p = 0.05; I2 = 69%), Asian patients have worse OS (HR 0.84; 95% CI 0.77, 0.92; p < 0.01; I2 = 15%) but not DSS (HR 0.81; 95% CI 0.31, 2.10; p = 0.66), Hispanic patients have no difference in OS (HR 1.03; 95% CI 0.79, 1.34; p = 0.66; I2 = 72%) or DSS (HR 2.63; 95% CI 0.34, 20.34; p = 0.35), and Native American patients have no difference in OS (HR 2.16; 95% CI 0.80, 5.83; p = 0.13). Compared to men, women have no difference in OS (HR 1.03; 95% CI 0.93, 1.15; p = 0.53; I2 = 92%) nor DSS (HR 0.99; 95% CI 0.90, 1.08; p = 0.78; I2 = 1%).

Conclusions

Disparate BC survival outcomes after RC are present, with Black patients having poorer OS and DSS as compared to White patients. Asian patients have lower OS but not DSS. Survival outcomes do not appear to differentiate by gender. Significant heterogeneity in variable and outcome definitions limited our ability to perform meta-analyses involving other potentially important drivers and sources of disparate outcomes.
Antecedents and consequences of healthiness in café service: Moderating effect of health concern
Authors: Song Myungkeun , Lee Won Seok , Moon Joonho
Published: September 2022

This study aims to examine the antecedents of consequences of healthiness in the café business context. Additionally, this study attests to the moderating effect of one's concern for health (health concern) between healthiness and attitude. To attain a more vivid response, this research selected Starbucks coffeehouse as a case study. Hygiene, healthiness, and nutritional disclosure are the determinants of healthiness in the café business area. The consequences of healthiness are attitude and purchase intention for café products. Health concern is the moderating variable between healthiness and attitude in the context of café businesses. In order to test the association between attributes, a survey was used. Amazon Mechanical Turk was chosen to recruit survey participants. The valid observation for data analysis was 455 participants. For hypothesis testing, a structural equation model was implemented. Regarding the results, health concern is positively influenced by hygiene and organicness, but healthiness is negatively affected by nutritional disclosure. Moreover, it was found that health concern significantly moderates the relationship between healthiness and attitude, and attitude exerts a positive effect on purchase intention.
Food insecurity and its determinants in a vulnerable area of Santiago, Chile
Authors: Pinheiro Anna Christina , Quintiliano-Scarpelli Daiana , Araneda-Flores Jacqueline , Oliveira Rogerio Antonio de , Pizarro Tito , Suarez-Reyes Mónica , Marques de Oliveira Maria Rita
Published: September 2022

This study aims to identify the determinants associated to food insecurity in a group of households composed of schoolchildren and their mothers/caregivers, who lived in a low-to-medium community development index area of the city of Santiago, Chile with a high presence of migrant population. The non-probabilistic and convenience sample was made up of 646 people, who answered a series of surveys with the aim of characterizing this group in sociodemographic terms (sex, age, number of inhabitants in the household, place of food purchase, conditional or non-conditional state transfer program beneficiary status, persons in charge of purchasing food for the household, mothers/caregivers education level and basic knowledge of food and nutrition). To assess moderate-to-severe food insecurity and severe food insecurity, the Food Insecurity Experience Scale-FIES was applied between September and October 2021. Logistic regression analysis were used to carry out multivariate analyses, with the use of stepwise back-and-forward strategies for the selected variables and defining p < 0.05. These models were adjusted per number of inhabitants in the household. The results indicate that 25.4% of households presented moderate-to-severe food insecurity, and 6.4% severe food insecurity experience. The variables that presented significant odds of risk to food insecurity were being a migrant, low maternal education level, low performance on basic knowledge in nutrition and when the father was responsible for food purchases. Several public policies have been implemented in Chile during the most recent decades aimed at increasing access to healthier foods and the implementation of healthier food environments. Despite this, there are still social and economic health determinants that contribute to the risk of odds insecurity for the most vulnerable groups in the country, thus putting at risk the fulfillment of the human right to adequate food at risk.
Preoperative excessive lateral anterior tibial subluxation is related to posterior tibial tunnel insertion with worse sagittal alignment after anterior cruciate ligament reconstructions
Authors: Liu An , Ye Xiaojun , Li Congsun , Yang Weinan , Yan Shigui , Xin Zengfeng , Wu Haobo
Published: September 2022

Objective

To investigate whether preoperative lateral anterior tibial subluxation (LATS) measured from magnetic resonance imaging (MRI) can influence tibial insertion and postoperative sagittal alignment after anterior cruciate ligament reconstructions (ACLRs).

Methods

84 patients who underwent single-bundle ACLRs were retrospectively investigated. Among them, 39 patients (LATS of <6 mm) 23 patients (LATS of ≥6 mm and <10 mm) and 22 patients (excessive LATS of ≥10 mm) were defined as group 1, 2 and 3, respectively. LATS, the position of graft insertion into tibia as ratio of anterior-posterior width (AP ratio) and the sagittal graft angle (SGA) were postoperatively assessed from MRI at 2-year follow-up. Following linear regression analyses were employed.

Results

The group 3 exhibited the largest preoperative LATS and remained the most postoperative LATS. Moreover, the group 3 possessed the most posteriorly located tunnel insertion with the largest AP ratio and the most vertical graft orientation. Of all included patients, a moderate correlation was demonstrated between pre- and postoperative LATS (r = 0.635). A low correlation was observed between preoperative LATS and AP ratio (r = 0.300) and a moderate correlation was displayed between AP ratio and SGA (r = 0.656).

Conclusion

For ACL injuries with excessive LATS (≥10 mm), most posteriorly located tibial insertion was found out, and worse sagittal alignment containing high residual LATS was associated with more vertical graft orientation following ACLRs.
Shape Sensing for an UAV Composite Half-Wing: Numerical Comparison between Modal Method and Ko’s Displacement Theory
Authors: Valoriani Filippo , Esposito Marco , Gherlone Marco
Published: September 2022

Shape sensing is the reconstruction of the displacement field of a structure from some discrete surface strain measurements and is a key technology for structural health monitoring. The aim of this paper is to compare two approaches to shape sensing that have been shown to be more efficient, especially for aircraft structures applications, in terms of required input strain measurements: the Ko’s Displacement Theory and the Modal Method. An object of the shape-sensing analysis is the half-wing of a multirotor UAV. The approaches are summarized in order to set the framework for the numerical comparative investigation. Then, the multirotor UAV is presented and a finite element model of its half-wing is used to simulate the static response to straight-and-level flight conditions. For a given common set of surface strain measurement points, Ko’s Displacement Theory and the Modal Method are compared in terms of accuracy of the reconstructed half-wing deflection and twist angle. The Modal Method is shown to be more accurate than Ko’s Displacement Theory, especially for the evaluation of the deflection field. Further numerical analyses show that the Modal Method is influenced by the set of mode shapes included in the analysis and that excellent reconstructed deflections can be obtained with a reduced number of sensors, thus assessing the approach as an efficient shape-sensing tool for aircraft structures real applications.
Probiotics in Allergic Rhinitis Management: Is There a Positioning for Them?
Authors: Ciprandi Giorgio , Tosca Maria Angela
Published: September 2022

Allergic rhinitis (AR) is a widespread medical condition affecting up to 40% of the general population. Type 2 inflammation determines typical nasal symptoms. In addition, gut and respiratory dysbiosis are present in AR patients. Probiotics have several beneficial effects on immunity, inflammatory pathways, and anti-infective properties. Namely, probiotic supplementation could restore immune response, promote eubiosis, and switch off inflammation. Thus, probiotics have also been investigated in AR. In addition, there is accumulating evidence that some specific strains of probiotics may improve AR. Five meta-analyses on probiotics in AR management were consistently published in the first half of 2022. The conclusions, although not definitive, argue for the possible use of probiotics as part of an add-on strategy in managing patients with allergic rhinitis.
A Comparative Immunohistochemical Study of Wound Healing after Dental Diode Laser Treatment in the Rat Oral Mucosa
Authors: Kim Hye Rin , Son Keunbada , Son Young-Tak , Kim Yong-Gun , Lee Kyu-Bok , Lee Seung Cheol , Suh Jo-Young , Lee Jae Mok
Published: September 2022

This study aimed to examine the differences in healing patterns using two types of diode laser devices (laser A and laser B) and a steel scalpel for periodontal surgery through histological and immunohistochemical methods. Twenty 12-week-old male rats were assigned to three groups (3, 7, and 14 days). Square-shaped erosion wounds (2 × 2 mm2 diameter) were created on the hard palate of each rat. Two wounds were created using Laser A and a steel scalpel (Bard-Parker No. 15) on the right palate and using Laser B and a steel scalpel on the left side. Rats were sacrificed after 3, 7, and 14 days. Tissues were collected with a margin of 1 mm from the border of the erosional wound of the maxillary hard palate. Histological and immunohistochemical analyses were performed on the tissue samples after 3, 7, and 14 days. The tissue healing pattern and expression of inducible nitric oxide synthase (iNOS) and cluster of differentiation (CD) were observed under a light microscope. Statistical analysis was conducted using the Kruskal–Wallis H test for comparison among the groups (α = 0.05). In comparison to the wounds made with the scalpel, wounds treated with lasers A and B showed delayed healing patterns. There was no significant difference between the two laser treatment groups (p > 0.05). The expression of iNOS and CD68 was not significantly different among the three groups after 3 and 7 days (p > 0.05). On day 14, the groups treated with the dental diode lasers showed higher expression than the group treated with the steel scalpel, but no significant difference was observed (p > 0.05). Laser-induced wounds tended to heal slower than surgical wounds performed using a steel scalpel, but histological and immunohistochemical results showed no significant difference between the dental diode laser and scalpel groups.
BioTEA: Containerized Methods of Analysis for Microarray-Based Transcriptomics Data
Authors: Visentin Luca , Scarpellino Giorgia , Chinigò Giorgia , Munaron Luca , Ruffinatti Federico Alessandro
Published: September 2022

Tens of thousands of gene expression data sets describing a variety of model organisms in many different pathophysiological conditions are currently stored in publicly available databases such as the Gene Expression Omnibus (GEO) and ArrayExpress (AE). As microarray technology is giving way to RNA-seq, it becomes strategic to develop high-level tools of analysis to preserve access to this huge amount of information through the most sophisticated methods of data preparation and processing developed over the years, while ensuring, at the same time, the reproducibility of the results. To meet this need, here we present bioTEA (biological Transcript Expression Analyzer), a novel software tool that combines ease of use with the versatility and power of an R/Bioconductor-based differential expression analysis, starting from raw data retrieval and preparation to gene annotation. BioTEA is an R-coded pipeline, wrapped in a Python-based command line interface and containerized with Docker technology. The user can choose among multiple options—including gene filtering, batch effect handling, sample pairing, statistical test type—to adapt the algorithm flow to the structure of the particular data set. All these options are saved in a single text file, which can be easily shared between different laboratories to deterministically reproduce the results. In addition, a detailed log file provides accurate information about each step of the analysis. Overall, these features make bioTEA an invaluable tool for both bioinformaticians and wet-lab biologists interested in transcriptomics. BioTEA is free and open-source.
Comparison of Gut Bacterial Communities of Locusta migratoria manilensis (Meyen) Reared on Different Food Plants
Authors: Wang Qian , Liu Yusheng , Yin Xiangchu
Published: September 2022

Locusts, in particular Locusta migratoria manilensis (Meyen), have been associated with major damages in agriculture, forestry, and animal husbandry in China. At present, L. migratoria manilensis has been largely domesticated, being considered an edible insect in China. Feeding variety is one of the main characteristics of L. migratoria manilensis. It has been demonstrated that microorganisms inhabiting the insect gut impact nutrition, development, defense, and reproduction of the insect host. The aim of the present study was to search for the adaptation mechanism of L. migratoria manilensis feeding on four different food plants (goosegrass, maize leaves, soybean leaves, and pakchoi) and explore changes in the gut bacterial community structure of the insect at the fifth instar nymph stage. Proteobacteria and Firmicutes were the dominant phyla, whereas Kluyvera, Enterobacter, Pseudocitrobacter, Klebsiella, Cronobacter, Citrobacter, Lactococcus, and Weissella were the dominant genera in the gut of L. migratoria manilensis. Principal component analysis and permutational multivariate analysis of variance (PERMANOVA) revealed significant differences in the gut microbiota structure of L. migratoria manilensis fed on different food plants. Moreover, functional prediction analysis revealed that metabolic and cellular processes were the most enriched categories. Within the category of metabolic processes, the most enriched pathways were carbohydrate transport and metabolism; amino acid transport and metabolism; translation, ribosomal structure, and biogenesis; cell wall/membrane/envelope biogenesis; inorganic ion transport and metabolism; and energy production and conversion. Collectively, the present results revealed that the structure of gut bacterial communities in L. migratoria manilensis fed on different food plants is impacted by food plants, which may play an essential part in the adaptation of the host.
The Effects of Nitrogen Fertilisation on the Anatomical Properties of the Populus alba L. Clone ‘Villafranca’ Juvenile Wood
Authors: Ištok Iva , Potočić Nenad , Šefc Bogoslav , Sedlar Tomislav
Published: September 2022

This study investigates the effect of nitrogen fertilisation on the anatomical properties of the juvenile wood of the Populus alba L. clone ‘Villafranca’ from an experimental trial near the Drava River in Croatia. Nitrogen was applied for two consecutive years, and the immediate and potential post-treatment effects were investigated. The correlation between annual ring width (ARW) and individual wood anatomical properties was also examined. The fertilisation effect was confirmed after the first year of nitrogen application for all wood anatomical properties except the vessel lumen area (VLA). Fibre length (FL) was reduced, and double cell wall thickness (DCWT), ray area (RA), and cell wall area (CWA) increased. In contrast, the vessel lumen diameter (VLD) and vessel lumen area changed inconsistently between treatments. The second year of nitrogen application was determined to be effective for FL only. Due to the insignificant results in the second year of the application of nitrogen, the post-fertilisation effect of nitrogen fertilisation was not confirmed.
SGCNCMI: A New Model Combining Multi-Modal Information to Predict circRNA-Related miRNAs, Diseases and Genes
Authors: Yu Chang-Qing , Wang Xin-Fei , Li Li-Ping , You Zhu-Hong , Huang Wen-Zhun , Li Yue-Chao , Ren Zhong-Hao , Guan Yong-Jian
Published: September 2022

Computational prediction of miRNAs, diseases, and genes associated with circRNAs has important implications for circRNA research, as well as provides a reference for wet experiments to save costs and time. In this study, SGCNCMI, a computational model combining multimodal information and graph convolutional neural networks, combines node similarity to form node information and then predicts associated nodes using GCN with a distributive contribution mechanism. The model can be used not only to predict the molecular level of circRNA–miRNA interactions but also to predict circRNA–cancer and circRNA–gene associations. The AUCs of circRNA—miRNA, circRNA–disease, and circRNA–gene associations in the five-fold cross-validation experiment of SGCNCMI is 89.42%, 84.18%, and 82.44%, respectively. SGCNCMI is one of the few models in this field and achieved the best results. In addition, in our case study, six of the top ten relationship pairs with the highest prediction scores were verified in PubMed.
Alphaviruses in Immunotherapy and Anticancer Therapy
Authors: Lundstrom Kenneth
Published: September 2022

Alphaviruses have been engineered as expression vectors for vaccine development and gene therapy. Due to the feature of RNA self-replication, alphaviruses can provide exceptional direct cytoplasmic expression of transgenes based on the delivery of recombinant particles, naked or nanoparticle-encapsulated RNA or plasmid-based DNA replicons. Alphavirus vectors have been utilized for the expression of various antigens targeting different types of cancers, and cytotoxic and antitumor genes. The most common alphavirus vectors are based on the Semliki Forest virus, Sindbis virus and Venezuelan equine encephalitis virus, but the oncolytic M1 alphavirus has also been used. Delivery of immunostimulatory cytokine genes has been the basis for immunotherapy demonstrating efficacy in different animal tumor models for brain, breast, cervical, colon, lung, ovarian, pancreatic, prostate and skin cancers. Typically, therapeutic effects including tumor regression, tumor eradication and complete cure as well as protection against tumor challenges have been observed. Alphavirus vectors have also been subjected to clinical evaluations. For example, therapeutic responses in all cervical cancer patients treated with an alphavirus vector expressing the human papilloma virus E6 and E7 envelope proteins have been achieved.
Did Maxwell Dream of Electrical Bacteria?
Authors: Alfinito Eleonora , Cesaria Maura , Beccaria Matteo
Published: September 2022

We propose a model for bacterial Quorum Sensing based on an auxiliary electrostatic-like interaction originating from a fictitious electrical charge that represents bacteria activity. A cooperative mechanism for charge/activity exchange is introduced to implement chemotaxis and replication. The bacteria system is thus represented by means of a complex resistor network where link resistances take into account the allowed activity-flow among individuals. By explicit spatial stochastic simulations, we show that the model exhibits different quasi-realistic behaviors from colony formation to biofilm aggregation. The electrical signal associated with Quorum Sensing is analyzed in space and time and provides useful information about the colony dynamics. In particular, we analyze the transition between the planktonic and colony phases as the intensity of Quorum Sensing is varied.
Dehydration Stress Memory Genes in Triticum turgidum L. ssp. durum (Desf.)
Authors: Sadder Monther T. , Musallam Anas , Allouzi Majd , Duwayri Mahmud A.
Published: September 2022

Exposure to successive stress cycles can result in a variety of memory response patterns in several plant species. We have investigated a group of these patterns at both the transcriptional and physiological memory levels in durum wheat. The data revealed huge discrepancies between investigated durum wheat cultivars, which presumably are all drought tolerant. It was possible to generate a consensus memory response pattern for each cultivar, where Hourani 27 was the most tolerant followed by Balikh 2 and then Omrabi 5. When durum wheat homologs from rice and maize were compared, only 18% gave similar memory response patterns. The data would indicate the presence of potentially divergent memory mechanisms in different plant species and genotypes. Ultimately, a thorough examination is required for each genotype before giving solid memory-based conclusions that can be applied in plant breeding and agricultural management practices.
Impact of Fibres on the Mechanical and Durable Behaviour of Fibre-Reinforced Concrete
Authors: More Florence More Dattu Shanker , Subramanian Senthil Selvan
Published: September 2022

Numerous studies have been conducted recently on fibre reinforced concrete (FRC), a material that is frequently utilized in the building sector. The utilization of FRC has grown in relevance recently due to its enhanced mechanical qualities over normal concrete. Due to increased environmental degradation in recent years, natural fibres were developed and research is underway with the goal of implementing them in the construction industry. In this work, several natural and artificial fibres, including glass, carbon, steel, jute, coir, and sisal fibres are used to experimentally investigate the mechanical and durability properties of fibre-reinforced concrete. The fibres were added to the M40 concrete mix with a volumetric ratio of 0%, 0.5%, 1.0%, 1.5%, 2.0% and 2.5%. The compressive strength of the conventional concrete and fibre reinforced concrete with the addition of 1.5% steel, 1.5% carbon, 1.0% glass, 2.0% coir, 1.5% jute and 1.5% sisal fibres were 4.2 N/mm2, 45.7 N/mm2, 41.5 N/mm2, 45.7 N/mm2, 46.6 N/mm2, 45.7 N/mm2 and 45.9 N/mm2, respectively. Comparing steel fibre reinforced concrete to regular concrete results in a 13.69% improvement in compressive strength. Similarly, the compressive strengths were increased by 3.24%, 13.69%, 15.92%, 13.68% and 14.18% for carbon, glass, coir, jute, and sisal fibre reinforced concrete respectively when equated with plain concrete. With the optimum fraction of fibre reinforced concrete, mechanical and durability qualities were experimentally investigated. A variety of durability conditions, including the Rapid Chloride Permeability Test, water absorption, porosity, sorptivity, acid attack, alkali attack, and sulphate attack, were used to study the behaviour of fiber reinforced concrete. When compared to conventional concrete, natural fibre reinforced concrete was found to have higher water absorption and sorptivity. The rate of acid and chloride attacks on concrete reinforced with natural fibres was significantly high. The artificial fibre reinforced concrete was found to be more efficient than the natural fibre reinforced concrete. The load bearing capacity, anchorage and the ductility of the concrete improved with the addition of fibres. According to the experimental findings, artificial fibre reinforced concrete can be employed to increase the structure’s strength and longevity as well as to postpone the propagation of cracks. A microstructural analysis of concrete was conducted to ascertain its morphological characteristics.
Reply to Peña, A. Comment on “Küppers et al. Percutaneous Anorectoplasty (PARP)—An Adaptable, Minimal-Invasive Technique for Anorectal Malformation Repair. Children 2022, 9, 587”
Authors: Küppers Julia , van Eckert Viviane , Muensterer Nadine R. , Holler Anne-Sophie , Rohleder Stephan , Kawano Takafumi , Gödeke Jan , Muensterer Oliver J.
Published: September 2022

Thank you so much for your thoughtful comments [...]
A Fast High-Order Predictor–Corrector Method on Graded Meshes for Solving Fractional Differential Equations
Authors: Su Xinxin , Zhou Yongtao
Published: September 2022

In this paper, we focus on the computation of Caputo-type fractional differential equations. A high-order predictor–corrector method is derived by applying the quadratic interpolation polynomial approximation for the integral function. In order to deal with the weak singularity of the solution near the initial time of the fractional differential equations caused by the fractional derivative, graded meshes were used for time discretization. The error analysis of the predictor–corrector method is carefully investigated under suitable conditions on the data. Moreover, an efficient sum-of-exponentials (SOE) approximation to the kernel function was designed to reduce the computational cost. Lastly, several numerical examples are presented to support our theoretical analysis.
Recent Advances in Mechanical Reinforcement of Zwitterionic Hydrogels
Authors: Lin Weifeng , Wei Xinyue , Liu Sihang , Zhang Juan , Yang Tian , Chen Shengfu
Published: September 2022

As a nonspecific protein adsorption material, a strong hydration layer provides zwitterionic hydrogels with excellent application potential while weakening the interaction between zwitterionic units, leading to poor mechanical properties. The unique anti-polyelectrolyte effect in ionic solution further restricts the application value due to the worsening mechanical strength. To overcome the limitations of zwitterionic hydrogels that can only be used in scenarios that do not require mechanical properties, several methods for strengthening mechanical properties based on enhancing intermolecular interaction forces and polymer network structure design have been extensively studied. Here, we review the works on preparing tough zwitterionic hydrogel. Based on the spatial and molecular structure design, tough zwitterionic hydrogels have been considered as an important candidate for advanced biomedical and soft ionotronic devices.
The Matter from Which an Orange Colour Is Made: On the Arsenic Pigment Used in a Portuguese Mannerist Painting
Authors: Cruz António João , Melo Helena P. , Valadas Sara , Miguel Catarina , Candeias António
Published: September 2022

The painting The Descent from the Cross, painted in 1620 by Pedro Nunes (1586–1637), presents two large figures with orange-coloured fabrics with conservation problems. Through the analysis of two samples with several analytical techniques, especially scanning electron microscopy combined with X-ray spectroscopy and Raman microscopy, it was possible to conclude that the orange colour is due to a complex artificial pigment made of amorphous arsenic sulphide. It essentially consists of spherical particles obtained by sublimation and condensation, possibly from orpiment, which ended up being joined with irregularly shaped particles resulting from crushing of the residual fraction obtained by solidification and fusion. This is a rare documented case of the extensive use of artificial arsenic sulphides in European easel painting, especially outside Italy. The conservation problems can be explained by the great sensitivity of the arsenic sulphides to photodegradation and the formation of powdery compounds.