bluuebunny/crossref_metadata_2025 huggingface dataset:
Endogenous DNA Double-Strand Breaks during DNA Transactions: Emerging Insights and Methods for Genome-Wide Profiling
Authors: Bouwman Britta A. M. , Crosetto Nicola
Published: December 2018

DNA double-strand breaks (DSBs) jeopardize genome integrity and can—when repaired unfaithfully—give rise to structural rearrangements associated with cancer. Exogenous agents such as ionizing radiation or chemotherapy can invoke DSBs, but a vast amount of breakage arises during vital endogenous DNA transactions, such as replication and transcription. Additionally, chromatin looping involved in 3D genome organization and gene regulation is increasingly recognized as a possible contributor to DSB events. In this review, we first discuss insights into the mechanisms of endogenous DSB formation, showcasing the trade-off between essential DNA transactions and the intrinsic challenges that these processes impose on genomic integrity. In the second part, we highlight emerging methods for genome-wide profiling of DSBs, and discuss future directions of research that will help advance our understanding of genome-wide DSB formation and repair.
Enhancing the Anticancer Efficacy of Immunotherapy through Combination with Histone Modification Inhibitors
Authors: Sun Wanyu , Lv Shuting , Li Hong , Cui Wei , Wang Lihui
Published: December 2018

In the nucleus of each cell, the DNA is wrapped around histone octamers, forming the so-called “nucleosomal core particles”. The histones undergo various modifications that influence chromatin structure and function, including methylation, acetylation, ubiquitination, phosphorylation, and SUMOylation. These modifications, known as epigenetic modifications (defined as heritable molecular determinants of phenotype that are independent of the DNA sequence), result in alterations of gene expression and changes in cell behavior. Recent work has shown that epigenetic drugs targeting histone deacetylation or methylation modulate the immune response and overcome acquired resistance to immunotherapy. A number of combination therapies involving immunotherapy and epigenetic drugs, which target histone deacetylation or methylation, are currently under various clinical/pre-clinical investigations and have shown promising anticancer efficacy. These combination therapies may provide a new strategy for achieving sustained anticancer efficacy and overcoming resistance.
Regulation of Structure-Specific Endonucleases in Replication Stress
Authors: Kim Seong Min , Forsburg Susan L.
Published: December 2018

Replication stress results in various forms of aberrant replication intermediates that need to be resolved for faithful chromosome segregation. Structure-specific endonucleases (SSEs) recognize DNA secondary structures rather than primary sequences and play key roles during DNA repair and replication stress. Holliday junction resolvase MUS81 (methyl methane sulfonate (MMS), and UV-sensitive protein 81) and XPF (xeroderma pigmentosum group F-complementing protein) are a subset of SSEs that resolve aberrant replication structures. To ensure genome stability and prevent unnecessary DNA breakage, these SSEs are tightly regulated by the cell cycle and replication checkpoints. We discuss the regulatory network that control activities of MUS81 and XPF and briefly mention other SSEs involved in the resolution of replication intermediates.
The Yellow Stripe-Like (YSL) Gene Functions in Internal Copper Transport in Peanut
Authors: Dai Jing , Wang Nanqi , Xiong Hongchun , Qiu Wei , Nakanishi Hiromi , Kobayashi Takanori , Nishizawa Naoko K. , Zuo Yuanmei
Published: December 2018

Copper (Cu) is involved in fundamental biological processes for plant growth and development. However, Cu excess is harmful to plants. Thus, Cu in plant tissues must be tightly regulated. In this study, we found that the peanut Yellow Stripe-Like family gene AhYSL3.1 is involved in Cu transport. Among five AhYSL genes, AhYSL3.1 and AhYSL3.2 were upregulated by Cu deficiency in peanut roots and expressed mainly in young leaves. A yeast complementation assay suggested that the plasma membrane-localized AhYSL3.1 was a Cu-nicotianamine complex transporter. High expression of AhYSL3.1 in tobacco and rice plants with excess Cu resulted in a low concentration of Cu in young leaves. These transgenic plants were resistant to excess Cu. The above results suggest that AhYSL3.1 is responsible for the internal transport of Cu in peanut.
Mid-Century Daily Discharge Scenarios Based on Climate and Land Use Change in Ouémé River Basin at Bétérou Outlet
Authors: Emmanuel Lawin Agnidé , N’Tcha M’Po Yèkambèssoun , Biaou Chabi Angelbert , Komi Kossi , Hounguè Rita , Yao Kouassi Benjamin , Afouda Abel Akambi
Published: December 2018

This study evaluates the impacts of land use and climate changes on daily discharge in Ouémé river basin at Bétérou outlet. Observed rainfall and temperature over 2002–2008 and land use data of 2003 and 2007 were used. Corrected rainfall and temperature data, under RCP4.5 and RCP8.5 scenarios from regional climate model REMO were considered. Two land use scenarios from RIVERTWIN project were used. The first one, Land Use A (LUA), is characterized by stronger economic development, controlled urbanization, implementation of large-scale irrigation schemes, and 3.2% population growth per year. The other one, Land Use B (LUB), is characterized by a weak national economy, uncontrolled settlement, and farmland development as well as 3.5% population growth per year. Four climate and land use combined scenarios (LUA + RCP4.5, LUA + RCP8.5; LUB + RCP4.5, and LUB + RCP8.5) were used for forcing LISFLOOD hydrological model to estimate future discharges at 2050. As a result, during calibration and validation, the LISFLOOD model showed high ability to reproduce historical flows of Ouémé River at Bétérou outlet with Nash–Sutcliffe efficiencies greater than 90%. Future discharges simulations show general increase for all land use and climate combined scenarios for all time horizons until 2050. The increase is more exacerbated under the combined scenarios using LUB than the ones using LUA. Increase of river discharge varies between 7.1% and 52% compared to the mean of the reference period 2002–2004. These findings highlight growing challenges for water resources managers and planners. Moreover, they emphasize the need to address potential climate and land use changes’ impact on water resources. Then, developing water management plans, strategies to reduce flooding risks must be considered.
Mobile App-Based Health Promotion Programs: A Systematic Review of the Literature
Authors: Lee Mikyung , Lee Hyeonkyeong , Kim Youlim , Kim Junghee , Cho Mikyeong , Jang Jaeun , Jang Hyoeun
Published: December 2018

This study investigated the features and usefulness of mobile app-based health promotion programs for the general population. A comprehensive bibliographic search of studies on health promotion programs using mobile apps in peer-reviewed journals published in English up to November 2017 was performed using the PubMed, Embase, and CINAHL databases. The inclusion criteria were (1) randomized control trial designs; (2) assessed mobile app-based interventions to promote adult health conditions; 12 studies were ultimately included. The most common topics were diet and physical activity (n = 8) and overall healthy lifestyle improvement (n = 4). The purpose of the apps included providing feedback on one’s health status (n = 9) and monitoring individual health status or behavior change (n = 9). Across all studies, health outcomes were shown to be better for mobile app users compared to non-users. Mobile app-based health interventions may be an effective strategy for improving health promotion behaviors in the general population without diseases. This study suggests that mobile app use is becoming commonplace for a variety of health-promoting behaviors in addition to physical activity and weight control. Future research should address the feasibility and effectiveness of using mobile apps for health promotion in developing countries.
Medical Service Quality, Efficiency and Cost Control Effectiveness of Upgraded Case Payment in Rural China: A Retrospective Study
Authors: He Ruibo , Ye Ting , Wang Jing , Zhang Yan , Li Zhong , Niu Yadong , Zhang Liang
Published: December 2018

Background: As the principal means of reimbursing medical institutions, the effects of case payment still need to be evaluated due to special environments and short exploration periods, especially in rural China. Methods: Xi County was chosen as the intervention group, with 36,104, 48,316, and 59,087 inpatients from the years 2011 to 2013, respectively. Huaibin County acted as the control group, with 33,073, 48,122, and 51,325 inpatients, respectively, from the same period. The inpatients’ information was collected from local insurance agencies. After controlling for age, gender, institution level, season fixed effects, disease severity, and compensation type, the generalised additive models (GAMs) and difference-in-differences approach (DID) were used to measure the changing trends and policy net effects from two levels (the whole county level and each institution level) and three dimensions (cost, quality and efficiency). Results: At the whole-county level, the cost-related indicators of the intervention group showed downward trends compared to the control group. Total spending, reimbursement fee and out-of-pocket expense declined by ¥346.59 (p < 0.001), ¥105.39 (p < 0.001) and ¥241.2 (p < 0.001), respectively (the symbol ¥ represents Chinese yuan). Actual compensation ratio, length of stay, and readmission rates exhibited ascending trends, with increases of 7% (p < 0.001), 2.18 days (p < 0.001), and 1.5% (p < 0.001), respectively. The intervention group at county level hospital had greater length of stay reduction (¥792.97 p < 0.001) and readmission rate growth (3.3% p < 0.001) and lower reimbursement fee reduction (¥150.16 p < 0.001) and length of stay growth (1.24 days p < 0.001) than those at the township level. Conclusions: Upgraded case payment is more reasonable and suitable for rural areas than simple quota payment or cap payment. It has successfully curbed the growth of medical expenses, improved the efficiency of medical insurance fund utilisation, and alleviated patients’ economic burden of disease. However, no positive effects on service quality and efficiency were observed. The increase in readmission rate and potential hidden dangers for primary health care institutions should be given attention.
Barriers from Multiple Perspectives Towards Physical Activity, Sedentary Behaviour, Physical Activity and Dietary Habits When Living in Low Socio-Economic Areas in Europe. The Feel4Diabetes Study
Authors: Van Stappen Vicky , Latomme Julie , Cardon Greet , De Bourdeaudhuij Ilse , Lateva Mina , Chakarova Nevena , Kivelä Jemina , Lindström Jaana , Androutsos Odysseas , González-Gil Esther , De Miguel-Etayo Pilar , Nánási Anna , Kolozsvári László , Manios Yannis , De Craemer Marieke ,   On behalf of the Feel4Diabetes-Study Group
Published: December 2018

This study investigated barriers towards health behaviours (physical activity, limiting sedentary behaviour and healthy dietary habits) experienced by young European families living in vulnerable areas, from multiple perspectives (parents, teachers, local community workers). Focus groups were conducted in six European countries (Belgium, Bulgaria, Finland, Hungary, Greece and Spain). In each country, three focus groups were conducted with parents, one with teachers and one with local community workers. Data were analysed using a deductive framework approach with a manifest content analysis using the software NVivo. The present study identified barriers on four levels (individual, interpersonal, organisational and macro level) of a socio-ecological model of health behaviour. From parents’ perspectives, both general barriers (e.g., financial limitations and lack of time) and country-specific barriers (e.g., organisational difficulties and inappropriate work environment) were identified. Additional barriers (e.g., lack of parental knowledge and lack of parental skills) were provided by other stakeholders (i.e., teachers and local community workers). The results of this study demonstrate the additional value of including multiple perspectives when developing a lifestyle intervention aiming to prevent type 2 diabetes in vulnerable groups. Future lifestyle interventions are recommended to include multiple components (family, school, and community) and could be implemented across European countries if country-specific adaptations are allowed.
Relationships between Microbial Indicators and Pathogens in Recreational Water Settings
Authors: Korajkic Asja , McMinn Brian R. , Harwood Valerie J.
Published: December 2018

Fecal pollution of recreational waters can cause scenic blight and pose a threat to public health, resulting in beach advisories and closures. Fecal indicator bacteria (total and fecal coliforms, Escherichia coli, and enterococci), and alternative indicators of fecal pollution (Clostridium perfringens and bacteriophages) are routinely used in the assessment of sanitary quality of recreational waters. However, fecal indicator bacteria (FIB), and alternative indicators are found in the gastrointestinal tract of humans, and many other animals and therefore are considered general indicators of fecal pollution. As such, there is room for improvement in terms of their use for informing risk assessment and remediation strategies. Microbial source tracking (MST) genetic markers are closely associated with animal hosts and are used to identify fecal pollution sources. In this review, we examine 73 papers generated over 40 years that reported the relationship between at least one indicator and one pathogen group or species. Nearly half of the reports did not include statistical analysis, while the remainder were almost equally split between those that observed statistically significant relationships and those that did not. Statistical significance was reported less frequently in marine and brackish waters compared to freshwater, and the number of statistically significant relationships was considerably higher in freshwater (p < 0.0001). Overall, significant relationships were more commonly reported between FIB and pathogenic bacteria or protozoa, compared to pathogenic viruses (p: 0.0022–0.0005), and this was more pronounced in freshwater compared to marine. Statistically significant relationships were typically noted following wet weather events and at sites known to be impacted by recent fecal pollution. Among the studies that reported frequency of detection, FIB were detected most consistently, followed by alternative indicators. MST markers and the three pathogen groups were detected least frequently. This trend was mirrored by reported concentrations for each group of organisms (FIB > alternative indicators > MST markers > pathogens). Thus, while FIB, alternative indicators, and MST markers continue to be suitable indicators of fecal pollution, their relationship with waterborne pathogens, particularly viruses, is tenuous at best and influenced by many different factors such as frequency of detection, variable shedding rates, differential fate and transport characteristics, as well as a broad range of site-specific factors such as the potential for the presence of a complex mixture of multiple sources of fecal contamination and pathogens.
Frailty as the Future Core Business of Public Health: Report of the Activities of the A3 Action Group of the European Innovation Partnership on Active and Healthy Ageing (EIP on AHA)
Authors: Liotta Giuseppe , Ussai Silvia , Illario Maddalena , O’Caoimh Rónán , Cano Antonio , Holland Carol , Roller-Winsberger Regina , Capanna Alessandra , Grecuccio Chiara , Ferraro Mariacarmela , Paradiso Francesca , Ambrosone Cristina , Morucci Luca , Scarcella Paola , De Luca Vincenzo , Palombi Leonardo
Published: December 2018

Background: The prevalence of frailty at population-level is expected to increase in Europe, changing the focus of Public Health. Here, we report on the activities of the A3 Action Group, focusing on managing frailty and supporting healthy ageing at community level. Methods: A three-phased search strategy was used to select papers published between January 2016 and May 2018. In the third phase, the first manuscript draft was sent to all A3-Action Group members who were invited to suggest additional contributions to be included in the narrative review process. Results: A total of 56 papers were included in this report. The A3 Action Group developed three multidimensional tools predicting short–medium term adverse outcomes. Multiple factors were highlighted by the group as useful for healthcare planning: malnutrition, polypharmacy, impairment of physical function and social isolation were targeted to mitigate frailty and its consequences. Studies focused on the management of frailty highlighted that tailored interventions can improve physical performance and reduce adverse outcomes. Conclusions: This review shows the importance of taking a multifaceted approach when addressing frailty at community level. From a Public Health perspective, it is vital to identify factors that contribute to successful health and social care interventions and to the health systems sustainability.
The More Educated, the Healthier: Evidence from Rural China
Authors: Wang Weidong , Dong Yongqing , Liu Xiaohong , Zhang Linxiu , Bai Yunli , Hagist Spencer
Published: December 2018

Education, as an important aspect of human capital, not only affects the economic returns of an individual, but also affects non-economic returns. This paper uses data from the China Family Panel Studies (CFPS) in 2014 and explores the impact of education on the health status of rural residents by using the family fixed-effect model. We find that education can improve the self-reported health status and reduce the possibility of depression of rural residents. We also find that the effect of education on self-reported health status of rural young people more significant than that of middle-aged and old people, but the effect on depression score was weaker than that of middle-aged and old people. Compared with the high-income group, education improved the health of the lowest income group more significantly. Finally, we explore the mechanism of education affecting the health of rural residents from a multi-dimensional perspective.
Barriers to Postpartum Glucose Intolerance Screening in an Italian Population
Authors: Quaresima Paola , Visconti Federica , Chiefari Eusebio , Puccio Luigi , Foti Daniela P. , Venturella Roberta , Vero Raffaella , Brunetti Antonio , Di Carlo Costantino
Published: December 2018

Background: Gestational diabetes mellitus (GDM) is a strong risk factor for type 2 diabetes mellitus (T2D) and the postpartum period is crucial for early treatment in at-risk women. However, despite recommendations, only a fraction of women undergo a postpartum screening for glucose intolerance (ppOGTT). The present study aims to verify the reason(s) for poor adherence in our population. Research design and methods: This retrospective study includes 451 women in which GDM was diagnosed between 2015–2016. During 2017, we verified by phone interview how many women underwent ppOGTT at 6–12 weeks postpartum, as recommended by the Italian guidelines. The non-compliant women were asked about the reason(s) for failing to screen. The non-parametric Mann-Whitney test and the 2-tailed Fisher exact test were used to compare continuous and categorical features, respectively, among women performing or non-performing ppOGTT. Results: Out of 451 women with GDM diagnosis, we recorded information from 327. Only 97 (29.7%) performed ppOGTT. The remaining 230 women (70.3%) provided the following explanation for non-compliance: (1) newborn care (30.4%); (2) misunderstood importance (28.3%); (3) oversight (13.0%); (4) unavailability of test reservation in the nearest centers (10.4%); (5) normal glycemic values at delivery (8.3%); (6) discouragement by primary care physician (5.6%). Conclusions: In our population, most women with recent GDM failed to perform ppOGTT. Our results indicated that the prominent barriers could potentially be overcome.
Heavy Metal Accumulation in Common Aquatic Plants in Rivers and Lakes in the Taihu Basin
Authors: Bai Li , Liu Xiao-Long , Hu Jian , Li Jun , Wang Zhong-Liang , Han Guilin , Li Si-Liang , Liu Cong-Qiang
Published: December 2018

We investigated the concentrations of 10 heavy metals in Potamogeton malaianus, Nymphoides peltata, Eichhornia crassipes, and Hydrilla verticillata to evaluate their potential to bioaccumulate heavy metals and related influencing factors in Taihu Lake. Enrichment factor (EF) values of Cu, Cr, Mn, Ni, Zn, Co, Pb, and V were above 2.0, indicating moderate to significant contamination in sediment. Most of Ti, V, Cr, Mn, and Ni in P. malaianus, E. crassipes, and H. verticillata and V in N. peltata were within excess/toxic level in plants, but higher than normal level. Even though no aquatic plants in this study were identified as a hyperaccumulator, relatively higher concentrations in aquatic plants were found in Taihu Lake than have been found in other previous studies. Heavy metal in submerged plants, especially in their stems, seemed to be more closely related to metals in water and sediment than those in floating-leaf plants. Ratios of metals in stem versus leaves in all plants ranged from 0.2 to 25.8, indicating various accumulation capabilities of plant organs. These findings contribute to the application of submerged aquatic plants to heavy metal removal from moderately contaminated lakes.
What Predicts Stable Mental Health in the 18–29 Age Group Compared to Older Age Groups? Results from the Stockholm Public Health Cohort 2002–2014
Authors: Winzer Regina , Sorjonen Kimmo , Lindberg Lene
Published: December 2018

Mental health has decreased in young people since the 1990s, and mental health promotion is an urgent matter. A first step is to identify which social determinants could be of importance for intervention. We used the Stockholm Public Health Cohort, a longitudinal population-based health survey, completed by 31,000 inhabitants in the Stockholm County. We focused on the 18–29 age group, n = 3373 (60% females, 40% males) and aimed at assessing which social determinants predict stable mental health, measured as scoring <3 points on the General Health Questionnaire 12 at all time points: 2002, 2007, 2010, and 2014. Forty-six percent of males and 36% of females reported stable mental health. Among the 17 predictors on sociodemographics, socioeconomics, social capital, health behavior, and victimization, six predicted stable mental health in the following order: occupation and especially employment, emotional support, male gender, being born in Sweden, absence of financial strain, and consumption of fruit and berries. In the 30–84 age group, 66% males and 55% females reported stable mental health. Nine determinants in the following rank predicted stable mental health: absence of financial strain, occupation and especially being self-employed, emotional support, male gender, physical activity, instrumental support, interpersonal trust, community trust, and absence of hazardous alcohol consumption. Interaction analysis showed significant difference between the younger and older group regarding physical activity and absence of financial strain with importance being higher for the older group. Our findings indicate that the determinants of health differ across the life-course with fewer predictors related to social capital and health behavior in the younger group compared to the older. We conclude that health-promoting interventions should be lifespan-sensitive.
Association of Exposure to Fine-Particulate Air Pollution and Acidic Gases with Incidence of Nephrotic Syndrome
Authors: Lin Shih-Yi , Hsu Wu-Huei , Lin Cheng-Li , Lin Cheng-Chieh , Lin Chih-Hsueh , Wang I-Kuan , Hsu Chung-Y. , Kao Chia-Hung
Published: December 2018

Background: Air pollution has been associated with autoimmune diseases. Nephrotic syndrome is a clinical manifestation of immune-mediated glomerulopathy. However, the association between nephrotic syndrome and air pollution constituents remains unknown. We conducted this nationwide retrospective study to investigate the association between PM2.5 and nephrotic syndrome. Methods: We used the Longitudinal Health Insurance Database (LHID) and the Taiwan Air Quality-Monitoring Database (TAQMD). We combined and stratified the LHID and the TAQMD data by residential areas of insurants linked to nearby air quality-monitoring stations. Air pollutant concentrations were grouped into four levels based on quartile. Univariable and multivariable Cox proportional hazard regression models were applied. Findings: Relative to Q1-level SO2, subjects exposed to the Q4 level were associated with a 2.00-fold higher risk of nephrotic syndrome (adjusted HR = 2.00, 95% CI = 1.66–2.41). In NOx, relative to Q1 NOx concentrations, the adjusted HRs of nephrotic syndrome risk were 1.53 (95% CI = 1.23–1.91), 1.30 (95% CI = 1.03–1.65), and 2.08 (95% CI = 1.69–2.56) for Q2, Q3, and Q4 levels, respectively. The results revealed an increasing trend for nephrotic syndrome risk correlating with increasing levels of NO, NO2, and PM2.5 concentrations. Interpretation: High concentrations of PM2.5, NO, NO2, and SO2 are associated with increased risk of nephrotic syndrome.
Environmental Health Oriented Optimal Temperature Control for Refrigeration Systems Based on a Fruit Fly Intelligent Algorithm
Authors: Qin Yuxiao , Sun Li , Hua Qingsong
Published: December 2018

The recent decades have witnessed refrigeration systems playing an important role in the life of human beings, with wide applications in various fields, including building comfort, food storage, food transportation and the medical special care units. However, if the temperature is not controlled well, it will lead to many harmful public health effects, such as the human being catching colds, food spoilage and harm to the recovering patients. Besides, refrigeration systems consume a significant portion of the whole society’s electricity usage, which consequently contributes a considerable amount of carbon emissions into the public environment. In order to protect human health and improve the energy efficiency, an optimal control strategy is designed in this paper with the following steps: (1) identifying the refrigeration system model based on a least squares method; (2) tuning an initial group of parameters of the proportional-integral-derivative (PID) controller via the pidTuner Toolbox of Matlab; (3) using an intelligent algorithm, namely fruit fly optimization (FOA), to further optimize the parameters of the PID controller. By comparing the optimal PID controller and the controller provided in the reference, the simulation results demonstrate that the proposed optimal PID controller can produce a more controllable temperature, with less tacking overshoot, less settling time, and more stable performance under a constant set-point.
An Assessment of Magnitudes and Patterns of Socioeconomic Inequalities across Various Health Problems: A Large National Cross-Sectional Survey in Korea
Authors: Shin Ji-Yeon , Lim Jiseun , Ki Myung , Song Yeong-Jun , Chun Heeran , Kim Dongjin
Published: December 2018

Magnitudes of health inequalities present consequences of socioeconomic impact on each health problem. To provide knowledge on the size of health problems in terms of socioeconomic burden, we examined the magnitudes and patterns of health inequalities across 12 health problems. A total of 17,292 participants older than 30 years were drawn from the Korea National Health and Nutrition Examination Survey (KNHANES, 2010–2012). The age-adjusted prevalence ratios were compared across socioeconomic positions (SEPs) based on income, education, and occupation. The magnitudes of socioeconomic inequalities varied across 12 health problems and, in general, the patterns of socioeconomic inequalities were similar among groups of health problems (i.e., non-communicable diseases (NCDs), mental health, and subjective health states). Significant health inequalities across NCDs, such as diabetes, hypertension, ischemic heart disease, and arthritis, were observed mainly in women. Socioeconomic inequalities in mental health problems, such as depression, suicidal ideation, and suicide attempts, were profound for both genders and across SEP measures. Significant socioeconomic inequalities were also observed for subjective health. No or weak associations were observed for injury and HBV infection. The patterns of socioeconomic inequalities were similar among groups of health problems. Mental illnesses appeared to require prioritization of socioeconomic approaches for improvement in terms of absolute prevalence and relative socioeconomic distribution.
A Comparison of Mercury Exposure from Seafood Consumption and Dental Amalgam Fillings in People with and without Amyotrophic Lateral Sclerosis (ALS): An International Online Case-Control Study
Authors: Parkin Kullmann Jane , Pamphlett Roger
Published: December 2018

Exposures to toxic metals such as mercury have been suggested to be risk factors for amyotrophic lateral sclerosis (ALS). Human intake of mercury commonly occurs via consumption of seafood or from mercury-containing amalgam dental restorations (‘mercury fillings’). We therefore compared mercury exposures from these sources in 401 ALS and 452 non-ALS respondents, using an internationally-available online questionnaire that asked respondents how often they ate seafood and what their favourite types of seafoods were. Respondents were also asked to record numbers of current or former mercury fillings. ALS and non-ALS respondents did not differ in their frequency of seafood consumption or in monthly mercury intake from favourite seafoods. Both groups had similar numbers of current, as well as former, mercury fillings. In conclusion, this study found no evidence that mercury exposure from eating seafood, or from mercury dental fillings, was associated with the risk of developing ALS. Therefore, if mercury does play a role in the pathogenesis of ALS, other sources of exposure to mercury in the environment or workplace need to be considered. Alternatively, a susceptibility to mercury toxicity in ALS, such as genetic or epigenetic variations, multiple toxic metal interactions, or selenium deficiency, may be present.
European Club Football after “Five Treatments” with Financial Fair Play—Time for an Assessment
Authors: Franck Egon
Published: December 2018

UEFA’s Club Licensing and Financial Fair Play Regulations (FFP) have impacted European club football. After five distinct applications of the break-even requirement, which represents the cornerstone of these regulations, it is time for an assessment. How has the situation in European top-division football changed since the FFP regulation? The most recent financial data show that European club football is characterized by significant financial recovery and further polarization. How has the FFP regulation presumably affected this development? This article discusses plausible reasons why FFP has contributed to financial recovery but has not aggravated polarization. Understanding the drivers of polarization is essential before taking further regulatory steps.
Differential Responses of OsMPKs in IR56 Rice to Two BPH Populations of Different Virulence Levels
Authors: Nanda Satyabrata , Wan Pin-Jun , Yuan San-Yue , Lai Feng-Xiang , Wang Wei-Xia , Fu Qiang
Published: December 2018

The conserved mitogen-activated protein kinase (MAPK) cascades play vital roles in plant defense responses against pathogens and insects. In the current study, the expression profiles of 17 OsMPKs were determined in the TN1 and IR56 rice varieties under the infestation of brown planthopper (BPH), one of the most destructive hemimetabolous rice pests. The virulent IR56 BPH population (IR56-BPH) and the avirulent TN1 BPH population (TN-BPH) were used to reveal the roles of OsMPKs in the compatible (IR56-BPH infested on the TN1 and IR56 rice varieties, and TN1-BPH infested on the TN1 rice variety) and the incompatible (TN1-BPH infested on the IR56 rice variety) interaction. The statistical analysis revealed that rice variety, BPH population type, and infestation period have significant effects on the transcription of OsMPKs. Out of these genes, five OsMPKs (OsMPK1, OsMPK3, OsMPK7, OsMPK14, and OsMPK16) were found to exhibit upregulated expression only during incompatible interaction. Six OsMPKs (OsMPK4, OsMPK5, OsMPK8, OsMPK9, OsMPK12, and OsMPK13) were associated with both incompatible and compatible interactions. The transcription analysis of salicylic acid, jasmonic acid, and ethylene phytohormone signaling genes revealed their roles during the rice–BPH interactions. The upregulated expression of OsC4H, OsCHS, and OsCHI in the incompatible interaction implied the potential defense regulatory roles of phenylpropanoids. In both varieties, the elevated transcript accumulations of OsGST and OsSOD, and the increased enzyme activities of POD, SOD, and GST at 1 day post-infestation (dpi), but not at 3 dpi, indicated that reactive oxygen species (ROS) signaling might be an early event in rice–BPH interactions. Furthermore, upregulated transcription of OsLecRK3 and OsLecRK4 was found only during an incompatible interaction, suggesting their involvement in the BPH resistance response in the IR56 rice variety. Lastly, based on the findings of this study, we have proposed a model of interactions of IR56 rice with TN1-BPH and IR56-BPH that depicts the resistance and susceptibility reactions, respectively.
Gamified Software to Support the Design of Business Innovation
Authors: De Nicola Antonio , Vicoli Giordano , Villani Maria Luisa
Published: December 2018

Business innovation is a process that requires creativity, and benefits from extensive collaboration. Currently, computational support in creativity processes is low, but modern techniques would allow these processes to be sped up. In this context, we provide such a computational support with software for business innovation design that uses computational creativity techniques. Furthermore, the software enables a gamified process to increase user engagement and collaboration, which mimics evolutionary methods, relying on a voting mechanism. The software includes a business innovation ontology representing the domain knowledge that is used to generate and select a set of diverse preliminary representations of business ideas. Indeed, the most promising for novelty and potential impact are identified to ignite a business innovation game where team members collaborate to elaborate new innovation ideas based on those inputs until convergence to a shortlist of business model proposals. The main features of the approach are illustrated by means of a running example concerning innovative services for smart cities.
Exploring How Homophily and Accessibility Can Facilitate Polarization in Social Networks
Authors: Taylor Cameron E. , Mantzaris Alexander V. , Garibay Ivan
Published: December 2018

Polarization in online social networks has gathered a significant amount of attention in the research community and in the public sphere due to stark disagreements with millions of participants on topics surrounding politics, climate, the economy and other areas where an agreement is required. This work investigates into greater depth a type of model that can produce ideological segregation as a result of polarization depending on the strength of homophily and the ability of users to access similar minded individuals. Whether increased access can induce larger amounts of societal separation is important to investigate, and this work sheds further insight into the phenomenon. Center to the hypothesis of homophilic alignments in friendship generation is that of a discussion group or community. These are modeled and the investigation into their effect on the dynamics of polarization is presented. The social implications demonstrate that initial phases of an ideological exchange can result in increased polarization, although a consensus in the long run is expected and that the separation between groups is amplified when groups are constructed with ideological homophilic preferences.
Larval Exposure to the Bacterial Insecticide Bti Enhances Dengue Virus Susceptibility of Adult Aedes aegypti Mosquitoes
Authors: Moltini-Conclois Isabelle , Stalinski Renaud , Tetreau Guillaume , Després Laurence , Lambrechts Louis
Published: December 2018

Understanding the interactions between pathogens sharing the same host can be complicated for holometabolous animals when larval and adult stages are exposed to distinct pathogens. In medically important insect vectors, the effect of pathogen exposure at the larval stage may influence susceptibility to human pathogens at the adult stage. We addressed this hypothesis in the mosquito Aedes aegypti, a major vector of arthropod-borne viruses (arboviruses), such as the dengue virus (DENV) and the chikungunya virus (CHIKV). We experimentally assessed the consequences of sub-lethal exposure to the bacterial pathogen Bacillus thuringiensis subsp. israelensis (Bti), during larval development, on arbovirus susceptibility at the adult stage in three Ae. aegypti strains that differ in their genetic resistance to Bti. We found that larval exposure to Bti significantly increased DENV susceptibility, but not CHIKV susceptibility, in the Bti-resistant strains. However, there was no major difference in the baseline arbovirus susceptibility between the Bti-resistant strains and their Bti-susceptible parental strain. Although the generality of our results remains to be tested with additional arbovirus strains, this study supports the idea that the outcome of an infection by a pathogen depends on other pathogens sharing the same host even when they do not affect the same life stage of the host. Our findings may also have implications for Bti as a mosquito biocontrol agent, indicating that the sub-optimal Bti efficacy may have counter-productive effects by increasing vector competence, at least for some combinations of arbovirus and mosquito strains.
Biomechanical Modeling of Wounded Skin
Authors: Chanda Arnab , Upchurch Weston
Published: December 2018

Skin injury is the most common type of injury, which manifests itself in the form of wounds and cuts. A minor wound repairs itself within a short span of time. However, deep wounds require adequate care and sometime clinical interventions such as surgical suturing for their timely closure and healing. In literature, mechanical properties of skin and other tissues are well known. However, the anisotropic behavior of wounded skin has not been studied yet, specifically with respect to localized overstraining and possibilities of rupture. In the current work, the biomechanics of common skin wound geometries were studied with a biofidelic skin phantom, using uniaxial mechanical testing and Digital Image Correlation (DIC). Global and local mechanical properties were investigated, and possibilities of rupture due to localized overstraining were studied across different wound geometries and locations. Based on the experiments, a finite element (FE) model was developed for a common elliptical skin wound geometry. The fidelity of this FE model was evaluated with simulation of uniaxial tension tests. The induced strain distributions and stress-stretch responses of the FE model correlated very well with the experiments (R2 > 0.95). This model would be useful for prediction of the mechanical response of common wound geometries, especially with respect to their chances of rupture due to localized overstraining. This knowledge would be indispensable for pre-surgical planning, and also in robotic surgeries, for selection of appropriate wound closure techniques, which do not overstrain the skin tissue or initiate tearing.
3D Imaging of Indentation Damage in Bone
Authors: Lowe Tristan , Avcu Egemen , Bousser Etienne , Sellers William , Withers Philip J.
Published: December 2018

Bone is a complex material comprising high stiffness, but brittle, crystalline bio-apatite combined with compliant, but tough, collagen fibres. It can accommodate significant deformation, and the bone microstructure inhibits crack propagation such that micro-cracks can be quickly repaired. Catastrophic failure (bone fracture) is a major cause of morbidity, particularly in aging populations, either through a succession of small fractures or because a traumatic event is sufficiently large to overcome the individual crack blunting/shielding mechanisms. Indentation methods provide a convenient way of characterising the mechanical properties of bone. It is important to be able to visualise the interactions between the bone microstructure and the damage events in three dimensions (3D) to better understand the nature of the damage processes that occur in bone and the relevance of indentation tests in evaluating bone resilience and strength. For the first time, time-lapse laboratory X-ray computed tomography (CT) has been used to establish a time-evolving picture of bone deformation/plasticity and cracking. The sites of both crack initiation and termination as well as the interconnectivity of cracks and pores have been visualised and identified in 2D and 3D.
Use of the Wilshire Equations to Correlate and Extrapolate Creep Data of Inconel 617 and Nimonic 105
Authors: Cedro III Vito , Garcia Christian , Render Mark
Published: December 2018

Advanced power plant alloys must endure high temperatures and pressures for durations at which creep data are often not available, necessitating the extrapolation of creep life. A recently developed creep life extrapolation method is the Wilshire equations, with which multiple approaches can be used to increase the goodness of fit of available experimental data and improve the confidence level of calculating long-term creep strength at times well beyond the available experimental data. In this article, the Wilshire equation is used to extrapolate the creep life of Inconel 617 and Nimonic 105 to 100,000 h. The use of (a) different methods to determine creep activation energy, (b) region splitting, (c) heat- and processing-specific tensile strength data, and (d) short-duration test data were investigated to determine their effects on correlation and extrapolation. For Inconel 617, using the activation energy of lattice self-diffusion as Q C * resulted in a poor fit with the experimental data. Additionally, the error of calculated rupture times worsened when splitting regions. For Nimonic 105, the error was reduced when heat- and processing-specific tensile strengths were used. Extrapolating Inconel 617 creep strength to 100,000 h life gave conservative results when compared to values calculated by the European Creep Collaborative Committee.
Chitosan/Poly(vinylpyrrolidone) Matrices Obtained by Gamma-Irradiation for Skin Scaffolds: Characterization and Preliminary Cell Response Studies
Authors: Casimiro Maria Helena , Gomes Susana R. , Rodrigues Gabriela , Leal João Paulo , Ferreira Luís M.
Published: December 2018

Several studies have shown that chitosan possesses characteristics favorable for promoting dermal regeneration and accelerated wound healing. In this work we have reported the work that has been done on the development and characterization of biocompatible and biodegradable chitosan based matrices to be used as skin scaffolds. Poly(vinylpyrrrolidone) (PVP) was used as copolymer and a two steps methodology of freeze-drying and gamma irradiation was used to obtain the porous matrices. The influence of PVP content, synthesis procedure and absorbed radiation dose on matrices’ physical, chemical and structural properties was evaluated by ATR-FTIR, TGA, SEM, contact angle measurements and degradation behavior. The in vitro cellular viability and proliferation of HFFF2 fibroblast cell line was analyzed as a measure of matrices’ biocompatibility and ability to assist skin regeneration. Results show that over the studied range values, gamma-radiation dose, copolymer concentration and synthesis procedure can be used to tailor the matrices’ morphology in terms of porosity and surface roughness. Early results from biological assays evidence the biocompatibility of the prepared chitosan/PVP matrices since cells adhered to the surface of all matrices (chitosan/PVP (5%) γ-irradiated at 10 kGy presents the higher cellular viability). These features show that the resultant matrices could be a potential suitable scaffold for skin tissue regeneration.
Extension of the ‘Inorganic Gel Casting’ Process to the Manufacturing of Boro-Alumino-Silicate Glass Foams
Authors: Rincon Romero Acacio , Tamburini Sergio , Taveri Gianmarco , Toušek Jaromír , Dlouhy Ivo , Bernardo Enrico
Published: December 2018

A new technique for the production of glass foams, based on alkali activation and gel casting, previously applied to soda-lime glass, was successfully extended to boro-alumino-silicate glass, recovered from the recycling of pharmaceutical vials. A weak alkali activation (2.5 M NaOH or NaOH/KOH aqueous solutions) of fine glass powders (below 70 µm) allowed for the obtainment of well-dispersed concentrated aqueous suspensions, undergoing gelation by treatment at low temperature (75 °C). Unlike soda-lime glass, the progressive hardening could not be attributed to the formation of calcium-rich silicate hydrates. The gelation was provided considering the chemical formulation of pharmaceutical glass (CaO-free) to the formation of hydrated sodium alumino-silicate (N-A-S-H) gel. An extensive direct foaming was achieved by vigorous mechanical stirring of partially gelified suspensions, comprising also a surfactant. A sintering treatment at 700 °C, was finally applied to stabilize the cellular structures.
Modification of a Defect-Based Fatigue Assessment Model for Al-Si-Cu Cast Alloys
Authors: Aigner Roman , Leitner Martin , Stoschka Michael , Hannesschläger Christian , Wabro Thomas , Ehart Robert
Published: December 2018

Cast parts usually inherit internal defects such as micro shrinkage pores due to the manufacturing process. In order to assess the fatigue behaviour in both finite-life and long-life fatigue regions, this paper scientifically contributes towards a defect-based fatigue design model. Extensive fatigue and fracture mechanical tests were conducted whereby the crack initiating defect size population was fractographically evaluated. Complementary in situ X-ray computed tomography scans before and during fatigue testing enabled an experimental estimation of the lifetime until crack initiation, acting as a significant input for the fatigue model. A commonly applied fatigue assessment approach introduced by Tiryakioglu was modified by incorporating the long crack threshold value, which additionally enabled the assessment of the fatigue strength in the long-life fatigue regime. The presented design concept was validated utilising the fatigue test results, which revealed a sound agreement between the experiments and the model. Only a minor deviation of up to about five percent in case of long-life fatigue strength and up to about 9% in case of finite-lifetime were determined. Thus, the provided extension of Tiryakioglu’s approach supports a unified fatigue strength assessment of cast aluminium alloys in both the finite- and long-life regimes.