bluuebunny/crossref_metadata_2025 huggingface dataset:
The Trans Middle Ages: Incorporating Transgender and Intersex Studies into the History of Medieval Sexuality
Authors: Wingard Tess
Published: January 2024

This article reviews recent work on trans and intersex history in the European Middle Ages, locating it within the established historiography on medieval gender and sexuality. It begins by surveying the three overarching themes in prior historical research on medieval gender and sexuality: identity, community and repression. Then, it introduces the context of the transgender turn in medieval studies, beginning in the late 2010s, by charting the origin of this sub-field and its rapid growth since 2020. It then outlines three key theoretical concepts underpinning its methodology: 1) gender is socially constructed and historically contingent; 2) likewise, biological sex is socially constructed and historically contingent; 3) all societies include people who transition away from their assigned gender, and, while these identities are no less contingent, they nevertheless constitute a valid subject for long histories of transness. Lastly, it argues that medieval trans and intersex studies contributes new perspectives and methodological approaches to each of the overarching research themes: 1) the lives and experiences of queer historical subjects such as Eleanor Rykener and Joseph of Schönau can be productively read through a trans lens, and trans historians’ focus on questions of agency and subjectivity provides a model for exploring queerness in the medieval archive; 2) trans historians’ arguments around community and mutual aid within trans case-studies revive the otherwise neglected historiographical topic of medieval queer networks; 3) trans historians demonstrate that medieval discourses around sexuality, gender and race are mutually constitutive, and significantly shape subsequent early modern European interactions with Black and indigenous societies.
Radioactive waste microbiology: predicting microbial survival and activity in changing extreme environments
Authors: Gregory Simon P , Mackie Jessica R M , Barnett Megan J
Published: January 2024

The potential for microbial activity to occur within the engineered barrier system (EBS) of a geological disposal facility (GDF) for radioactive waste is acknowledged by waste management organizations as it could affect many aspects of the safety functions of a GDF. Microorganisms within an EBS will be exposed to changing temperature, pH, radiation, salinity, saturation, and availability of nutrient and energy sources, which can limit microbial survival and activity. Some of the limiting conditions are incorporated into GDF designs for safety reasons, including the high pH of cementitious repositories, the limited pore space of bentonite-based repositories, or the high salinity of GDFs in evaporitic geologies. Other environmental conditions such as elevated radiation, temperature, and desiccation, arise as a result of the presence of high heat generating waste (HHGW). Here, we present a comprehensive review of how environmental conditions in the EBS may limit microbial activity, covering HHGW and lower heat generating waste (LHGW) in a range of geological environments. We present data from the literature on the currently recognized limits to life for each of the environmental conditions described above, and nutrient availability to establish the potential for life in these environments. Using examples where each variable has been modelled for a particular GDF, we outline the times and locations when that variable can be expected to limit microbial activity. Finally, we show how this information for multiple variables can be used to improve our understanding of the potential for microbial activity to occur within the EBS of a GDF and, more broadly, to understand microbial life in changing environments exposed to multiple extreme conditions.
Humanitarian–Development Nexus: strengthening health system preparedness, response and resilience capacities to address COVID-19 in Sudan—case study of repositioning external assistance model and focus
Authors: Mohamed Nur Muna , Aweesha Huzeifa , Elsharif Mahmoud , Esawi Ahmed , Omer Arwa , Musa Mohamed
Published: January 2024

The advent of the COVID-19 pandemic and the establishment of a new transitional government in Sudan with rejuvenated relations with the international community paved the way for external assistance to the EU COVID-19 response project, a project with a pioneering design within the region. The project sought to operationalize the humanitarian–development–peace nexus, perceiving the nexus as a continuum rather than sequential due to the protracted nature of emergencies in Sudan and their multiplicity and contextual complexity. It went further into enhancing peace through engaging with conflict and post-conflict-affected states and communities and empowering local actors. Learning from this experience, external assistance models to low- or middle-income countries (LMICs) should apply principles of flexibility and adaptability, while maintaining trust through transparency in exchange, to ensure sustainable and responsive action to domestic needs within changing contexts. Careful selection and diverse project team skills, early and continuous engagement with stakeholders, and robust planning, monitoring and evaluation processes were the project highlights. Yet, the challenges of political turmoil, changing Ministry of Health leadership, competing priorities and inactive coordination mechanisms had to be dealt with. While applying such an approach of a health system lens to health emergencies in LMICs is thought to be a success factor in this case, more robust technical guidance to the nexus implementation is crucial and can be best attained through encouraging further case reports analysing context-specific practices.
Identifying BMI-associated genes via a genome-wide multi-omics integrative approach using summary data
Authors: Tang Jingxian , Xu Hanfei , Xin Zihao , Mei Quanshun , Gao Musong , Yang Tiantian , Zhang Xiaoyu , Levy Daniel , Liu Ching-Ti
Published: January 2024

Objective

This study aims to identify BMI-associated genes by integrating aggregated summary information from different omics data.

Methods

We conducted a meta-analysis to leverage information from a genome-wide association study (n = 339 224), a transcriptome-wide association study (n = 5619), and an epigenome-wide association study (n = 3743). We prioritized the significant genes with a machine learning-based method, netWAS, which borrows information from adipose tissue-specific interaction networks. We also used the brain-specific network in netWAS to investigate genes potentially involved in brain-adipose interaction.

Results

We identified 195 genes that were significantly associated with BMI through meta-analysis. The netWAS analysis narrowed down the list to 21 genes in adipose tissue. Among these 21 genes, six genes, including FUS, STX4, CCNT2, FUBP1, NDUFS3, and RAPSN, were not reported to be BMI-associated in PubMed or GWAS Catalog. We also identified 11 genes that were significantly associated with BMI in both adipose and whole brain tissues.

Conclusion

This study integrated three types of omics data and identified a group of genes that have not previously been reported to be associated with BMI. This strategy could provide new insights for future studies to identify molecular mechanisms contributing to BMI regulation.
Increasing Exposure to Inflammatory Bowel Diseases Education in Gastroenterology Fellowship: The Pilot IBD 101 Experience
Authors: Malter Lisa , Hong Simon J , Lopatin Sarah , Murphy Megan , Hudesman David , Kane Sunanda , Rubin David T
Published: January 2024

Background

Inflammatory bowel disease (IBD) management has become increasingly complex, and education varies across fellowship programs. IBD 101 was designed to introduce first-year gastroenterology (GI) fellows to IBD care and training.

Methods

In 2019, a cohort of fellows participated in a 1-day course with small group learning and group observed structured clinical examinations. Pre- and postcourse surveys were administered to evaluate the course. To assess the long-term impact, surveys were emailed in May 2022 to all third-year fellows from previously participating programs. The primary outcome was comfort managing IBD scenarios and information regarding each fellow’s exposure to IBD education.

Results

Fifty-five fellows from 32 programs participated. A total of 49 (89%) of 55 completed pre- and postcourse surveys. All fellows agreed that the course content was appropriate. In the postcourse survey, all fellows reported increased comfort managing IBD patients. Ninety-six percent of attendees stated that they would strongly recommend this course. Thirty-six fellows completed surveys in 2022, 21 (58%) attendees and 15 (42%) nonattendees. Attendees reported equivalent or higher levels of comfort compared with nonattendees. Higher global competence was noted among attendees (odds ratio, 5.21; 95% confidence interval, 0.91-29.9; P = .06) after adjusting for presence of a local IBD specialist, number of IBD patients seen monthly (≤5 vs >5), and rotation through an IBD service.

Conclusions

IBD 101, an introductory course for first-year GI trainees, was associated with increased comfort managing IBD with a durable benefit independent of individual access to IBD education. Continuation of this program will further enhance the IBD education of future GI fellows.
Desinformación por razón de sexo y redes socialesGendered disinformation and social networks
Authors: Queralt Jiménez Argelia
Published: January 2024

The subject of this article is gender-based disinformation on social networks. This type of disinformation is identified with those contents that circulate on networks with the aim of attacking women for being women and expelling them from public debate. This type of behavior undermines women’s credibility in the public eye and can lead to their abandonment of the public sphere. Gendered disinformation particularly targets women who have an impact on the public conversation, politicians, journalists, activists or women with a voice of their own in the media and networks. This phenomenon is a major problem for our democracies because, firstly, it is a blatant demonstration of the discrimination suffered by women in general. And, secondly, because it means restricting the legitimate exercise of these women’s freedom of communication, in a broad sense, and participation in political life, which in turn leads to the distortion of pluralism and participation in decision-making processes.
Does an Annulled Award Constitute Legal Authority in Investment Arbitration?
Authors: van den Berg Albert Jan
Published: January 2024

Arbitration lawyers have been discussing enforcement of annulled awards for some 25 years. Recently, another discussion regarding annulled awards is gaining traction: can an investment arbitrator rely on an annulled award? More specifically, does it constitute legal authority? The author submits that, in investment arbitration, an arbitral award, annulled by a national court in the UNCITRAL Rules context or by an ad hoc committee in the ICSID context, does not constitute legal authority, nor can it give guidance on the content of the applicable law in another manner.
Does concomitant tricuspid valve intervention at the time of left ventricular assist device placement for patients with significant tricuspid regurgitation lead to improved outcomes compared to isolated left ventricular assist device placement?
Authors: Mitra Ananya , Castleberry Anthony , Urban Marian , Siddique Aleem
Published: January 2024

A best evidence topic in cardiac surgery was written according to a structured protocol. The question addressed was ‘In patients with advanced heart failure (HF) and moderate to severe functional tricuspid regurgitation (TR) undergoing left ventricular assist device (LVAD) placement is concomitant tricuspid valve intervention (TVI) superior for the clinical outcomes of survival, right ventricular failure, rehospitalizations for HF, functional status, and quality of life?’ Altogether, 56 papers were found using the reported search, of which 12 papers represented the best evidence to answer the clinical question. The authors, journal, date, country of publication, patient group studied, study type, relevant outcomes and results of these papers are tabulated. Our search found no significant clinical benefit for concomitant TVI at the time of LVAD placement. We conclude that patient with moderate-to-severe TR should not routinely undergo concomitant TVI with LVAD placement.
“If You Destroy Our Children, I Will Kill You”: Biopolitical Childhood in Southeast Asia’s War on Drugs
Authors: Raffle Euan
Published: January 2024

This article explores how the war on drugs in Southeast Asia upholds the protection of the young as a key justification for extrajudicial killings carried out by the state. As many as 30,000 extrajudicial killings took place during Philippine former President Rodrigo Duterte’s war on drugs between 2016 and 2022, echoing a similar anti-drug campaign in Thailand in 2003, which saw around 3,000 people murdered. Drawing on political speeches, this article argues that Duterte and Thai Prime Minister Thaksin Shinawatra deployed biopolitical patterns of discourse to legitimize the murder of those associated with the drug trade. Both leaders framed the threat of drugs to children through the biopolitical lens of the integrity of the family, national health, and therefore the future of the nation. Further, this article demonstrates how in the Philippine drug war, children were effectively denied their childhood status and presented as “dangerous becomings.” This reveals the contingent nature of childhood, as biopolitical criteria were also used to establish which children were perceived to be dangerous in the context of the drug war. In doing so, this paper serves to nuance and problematize how children are depicted in security studies, and international relations more broadly.
World Order Transformation from the Grassroots: Global South Social Movements and the Transcendence of Established Approaches to International Change
Authors: Davies Thomas
Published: January 2024

In contrast to traditional top-down perspectives, this article aims to shed alternative light on the prospects for change in global order through evaluating how perspectives offered among social movements located in the Global South consider how change can take place beyond established approaches. With reference to perspectives offered among the Global Tapestry of Alternatives, the article elucidates a model of global political change that transcends reformist and revolutionary dynamics, and which bypasses dominant state and intergovernmental institutions. The analysis highlights the ontological divide between these approaches and reformist and revolutionary perspectives, given their pluriversality drawing on concepts that to date have received inadequate attention in the study of international relations. The article further considers how these approaches—given their deep roots in long-established communitarian practices and their pluriversality—may be addressing today's crisis of global order from the bottom-up in ways that may avoid limitations of previous change agendas.
Determining a urinary-specific antibiogram and risk factors of trimethoprim/sulfamethoxazole, ciprofloxacin and multidrug resistance among Enterobacterales in primary care
Authors: Valentine-King Marissa , Hansen Michael A , Zoorob Roger , Schlueter Matthew , Matas Jennifer L , Willis Samuel E , Danek Lisa C K , Muldrew Kenneth , Zare Mohammad , Hudson Forrest , Atmar Robert L , Chou Andrew , Trautner Barbara W , Grigoryan Larissa
Published: January 2024

Background

Risk factors for ciprofloxacin or MDR in primary care urine specimens are not well defined.

Objectives

We created a primary care-specific antibiogram for Escherichia coli isolates from cases with complicated and uncomplicated urinary tract infection (UTI) and evaluated risk factors for ciprofloxacin, trimethoprim/sulfamethoxazole and MDR among Enterobacterales.

Methods

We conducted a cross-sectional study to determine resistance and risk factors by collecting urine cultures from all patients (≥18 years) presenting with provider-suspected UTI at two primary care, safety-net clinics in Houston, TX, USA between November 2018 and March 2020.

Results

Among 1262 cultures, 308 cultures grew 339 uropathogens. Patients with Enterobacterales (n = 199) were mostly female (93.5%) with a mean age of 48.5 years. E. coli was the predominant uropathogen isolated (n = 187/339; 55%) and had elevated trimethoprim/sulfamethoxazole (43.6%) and ciprofloxacin (29.5%) resistance, low nitrofurantoin (1.8%) resistance, and no fosfomycin resistance. Among E. coli, 10.6% were ESBL positive and 24.9% had MDR. Birth outside the U.S.A., prior (2 year) trimethoprim/sulfamethoxazole resistance, and diabetes mellitus were associated with trimethoprim/sulfamethoxazole resistance. Prior (60 day) fluoroquinolone use, prior ciprofloxacin resistance and both diabetes mellitus and hypertension were strongly associated with ciprofloxacin resistance. Prior fluoroquinolone use and a history of resistance to any studied antibiotic were associated with MDR, while pregnancy was protective.

Conclusions

We found elevated resistance to UTI-relevant antimicrobials and novel factors associated with resistance; these data can be incorporated into clinical decision tools to improve organism and drug concordance.
Structured and unstructured social risk factor documentation in the electronic health record underestimates patients’ self-reported risks
Authors: Iott Bradley E , Rivas Samantha , Gottlieb Laura M , Adler-Milstein Julia , Pantell Matthew S
Published: January 2024

Objectives

National attention has focused on increasing clinicians’ responsiveness to the social determinants of health, for example, food security. A key step toward designing responsive interventions includes ensuring that information about patients’ social circumstances is captured in the electronic health record (EHR). While prior work has assessed levels of EHR “social risk” documentation, the extent to which documentation represents the true prevalence of social risk is unknown. While no gold standard exists to definitively characterize social risks in clinical populations, here we used the best available proxy: social risks reported by patient survey.

Materials and Methods

We compared survey results to respondents’ EHR social risk documentation (clinical free-text notes and International Statistical Classification of Diseases and Related Health Problems [ICD-10] codes).

Results

Surveys indicated much higher rates of social risk (8.2%-40.9%) than found in structured (0%-2.0%) or unstructured (0%-0.2%) documentation.

Discussion

Ideally, new care standards that include incentives to screen for social risk will increase the use of documentation tools and clinical teams’ awareness of and interventions related to social adversity, while balancing potential screening and documentation burden on clinicians and patients.

Conclusion

EHR documentation of social risk factors currently underestimates their prevalence.
Approaching virtual osteoid volume estimation and in-depth tissue characterization in patients with tumor-induced osteomalacia
Authors: Schmidt Felix N , Delsmann Julian , Yazigi Bashar , Beil Frank Timo , Amling Michael , Oheim Ralf
Published: January 2024

Tumor-induced osteomalacia (TIO) poses a significant diagnostic challenge, leading to increased disease duration and patient burden also by missing clinical suspicion. Today, diagnosis of osteomalacia relies on invasive iliac crest biopsy, if needed. Therefore, a noninvasive method would be beneficial for patients with severe osteomalacia, such as TIO, to inform their clinical management and address specific needs, like estimating the regeneration capacity at high osteoid volumes (OVs) or the potential of a hungry bone syndrome after tumor removal. Furthermore, given the lack of comprehensive histological characterization of TIO, there is a need for additional tissue characterization. Therefore, our assessment encompassed iliac crest biopsies that were examined using quantitative electron backscattered microscopy, Raman spectroscopy, micro-computed tomography, and histology to analyze the biopsy tissue. Our clinical assessment encompassed DXA and high-resolution peripheral quantitative computed tomography (HR-pQCT) alongside with biochemical analyses and clinical evaluations. Combining imaging and clinical data, we established a model to predict the OV. We compared 9 TIO patients with 10 osteoporosis (OPO) patients and 10 healthy controls. Histological analyses confirmed a pronounced OV in TIO patients (OPO: 1.20% ± 1.23% vs TIO: 23.55% ± 12.23%, P < .0005), and spectroscopy revealed lower phosphate levels in TIO biopsies. By combining HR-pQCT and laboratory diagnostics, we developed a linear regression model to noninvasively predict the OV revealing significantly higher modeled OV/BVmodel values of 24.46% ± 14.22% for TIO compared to the control group (5.952% ± 3.44%, P ≤ .001). By combining laboratory diagnostics, namely, ALP and Tt.BMDRadius measured by HR-pQCT, we achieved the calculation of the virtual osteoid volume to bone volume ratio (OV/BVmodel) with a significant correlation to histology as well as reliable identification of TIO patients compared to OPO and control. This novel approach is potentially helpful for predicting OV by noninvasive techniques in diagnostic procedures and improving the clinical management of TIO.
A tool for live-cell confocal imaging of temperature-dependent organelle dynamics
Authors: Midorikawa Keiko , Kodama Yutaka
Published: January 2024

Intracellular organelles alter their morphology in response to ambient conditions such as temperature to optimize physiological activities in cells. Observing organelle dynamics at various temperatures deepens our understanding of cellular responses to the environment. Confocal laser microscopy is a powerful tool for live-cell imaging of fluorescently labeled organelles. However, the large contact area between the specimen and the ambient air on the microscope stage makes it difficult to maintain accurate cellular temperatures. Here, we present a method for precisely controlling cellular temperatures using a custom-made adaptor that can be installed on a commercially available temperature-controlled microscope stage. Using this adaptor, we observed temperature-dependent organelle dynamics in living plant cells; morphological changes in chloroplasts and peroxisomes were temperature dependent. This newly developed adaptor can be easily placed on a temperature-controlled stage to capture intracellular responses to temperature at unprecedentedly high resolution.
Diabetes distress in urban Black youth with type 1 diabetes and their caregivers: associations with glycemic control, depression, and health behaviors
Authors: Evans Meredyth , Ellis Deborah A , Vesco Anthony T , Feldman Marissa A , Weissberg-Benchell Jill , Carcone April Idalski , Miller Jennifer , Boucher-Berry Claudia , Buggs-Saxton Colleen , Degnan Bernard , Dekelbab Bassem , Drossos Tina
Published: January 2024

Objectives

Adolescents with type 1 diabetes (T1D) and their caregivers endorse high diabetes distress (DD). Limited studies have documented the impact of DD on Black youth. The aims of the present study were to (1) describe DD among a sample of Black adolescents with T1D and their caregivers, (2) compare their DD levels with published normative samples, and (3) determine how DD relates to glycemic outcomes, diabetes self-management, parental monitoring of diabetes, and youth depressive symptoms.

Methods

Baseline data from a multicenter clinical trial were used. Participants (N = 155) were recruited from 7 Midwestern pediatric diabetes clinics. Hemoglobin A1c (HbA1c) and measures of DD, parental monitoring of diabetes care, youth depression and diabetes management behaviors were obtained. The sample was split into (1) adolescents (ages 13–14; N = 95) and (2) preadolescents (ages 10–12; N = 60). Analyses utilized Cohen’s d effect sizes, Pearson correlations, t-tests, and multiple regression.

Results

DD levels in youth and caregivers were high, with 45%–58% exceeding either clinical cutoff scores or validation study sample means. Higher DD in youth and caregivers was associated with higher HbA1c, lower diabetes self-management, and elevated depressive symptoms, but not with parental monitoring of diabetes management.

Conclusions

Screening for DD in Black youth with T1D and caregivers is recommended, as are culturally informed interventions that can reduce distress levels and lead to improved health outcomes. More research is needed on how systemic inequities contribute to higher DD in Black youth and the strategies/policy changes needed to reduce these inequities.
Growth of condition-related knowledge among youth with spina bifida: associations with neurocognitive functioning and self-management skills
Authors: Clark Olivia E , Smith Zoe R , Hilderbrand Taylor , Holmbeck Grayson N
Published: January 2024

Objective

This study aims to characterize the growth in condition-related knowledge in youth with spina bifida (SB), identify neurocognitive predictors of growth, and examine associations between growth in knowledge and subsequent levels of medical self-management skills.

Methods

Participants were recruited from a larger longitudinal study involving 140 youth with SB and caregivers, who completed questionnaires and interviews every 2 years over 8 years. The current study included the youth report of condition-related knowledge and medical self-management skills. Youth attention and executive functioning were assessed via parent and teacher reports and performance-based assessment. Latent growth curves were conducted in Mplus Version 8 (Múthen, L. K., & Múthen, B. O. [1998]. Mplus User’s Guide. [Eighth]. Muthén & Muthén) to examine change over time in youth-reported condition-related knowledge. Neurocognitive variables were included as predictors of growth in knowledge and regression analyses were used to predict medical self-management skills from growth in condition-related knowledge.

Results

Youth condition-related knowledge increased linearly. Better youth performance on working memory and attention performance-based tasks predicted a higher intercept for condition-related knowledge at T1, but not slope. Teacher and parent reports of inattention and executive dysfunction were not consistent predictors of intercept and growth. Slope of condition-related knowledge was not predictive of subsequent youth self-management skills.

Conclusions

Youth with SB gain condition-related knowledge over time. However, executive dysfunction and inattention may impede gains in condition-related knowledge. Thus, executive functioning supports, attention-related interventions, and psychoeducation may support condition-related knowledge gains and later medical self-management skills, but further research assessing family and cultural factors is needed.
Implementing point-of-care hemoglobin A1C testing in an obstetrics outpatient clinic
Authors: Weli Homayemem , Farnsworth Christopher W
Published: January 2024

Background

A1C ≥6.0% is associated with increased risk of adverse outcomes in pregnant diabetic patients. A1C testing is recommended by the American Diabetes Association as a secondary measure of glycemic control in pregnant patients.

Objective

To determine the utility of A1C point-of-care testing (POCT) during pregnancy to facilitate rapid counseling and diabetes care, particularly in relatively low-income transient patient populations.

Methods

We performed a single-center, retrospective analysis of patients presenting to an outpatient obstetrics office with routine, in-laboratory A1C testing, before and after the implementation of POCT for A1C (n = 70 and n = 75, respectively). Demographics, results, physician referral to a nutritionist, counseling, and outcomes were retrieved from patient electronic medical records.

Results

In total, 9% and 23% of the in-laboratory and POCT groups, respectively, were referred for nutrition services (P = .02). Of these, 22% of the in-laboratory group and 42% of the POCT group received immediate counseling (P < .01). An inverse correlation was observed between A1C level at study entry and gestational weeks at delivery, with a Pearson r value of −0.39 (−0.58 to −0.16) for the in-laboratory group and −0.38 (−0.57 to −0.14) for the POCT group. No statistically significant difference in pregnancy outcomes was observed.

Conclusion

Implementation of A1C POCT was associated with immediate counseling and management of the health of pregnant patients, but was not associated with improved outcomes, in a low-resource patient population.
Comparative analysis of a novel N-butanol-prepared antigen vs thermo-resistant and sonicated antigens for human leptospirosis detection
Authors: Blanco Roberta Morozetti , Lima Elaine dos Santos , de Haro Gabriela Nogueira , Kamikawa Camila Mika , Blanco Rodrigo Morozetti , Vincentini Adriana Pardini , Romero Eliete Caló
Published: January 2024

The diagnosis of human leptospirosis is mainly based on serological assays. Since the extraction by N-butanol has only been studied as an antigen for the diagnosis of cattle leptospirosis, this study aimed to investigate the feasibility of the N-butanol preparation for the diagnosis of human leptospirosis and compare it with sonicated and thermo-resistant antigens in IgM dot-blot test. Paired serum samples from 147 laboratory-confirmed leptospirosis cases were tested. The control group consisted of 148 serum samples from healthy individuals and nonleptospirosis cases. N-butanol antigens from serovar Copenhageni (ButC3) and serovar Patoc (ButP3) showed reactivity with antileptospiral antibodies from patients with confirmed leptospirosis. In the acute phase, sensitivities of IgM dot-blot assay with ButC3 and ButP3 antigens were 47.6% and 51.0%, respectively. In the convalescent phase, sensitivities were 95.9% (ButC3) and 93.2% (ButP3), and no significant differences were observed among the IgM dot-blot tests with other antigens. The specificity of the IgM dot-blot test with ButC3 antigen was good (92.6%), but with ButP3 (83.1%), it was significantly lower than with the other tests. The IgM dot-blot test described in this study is simple to perform and presents reliable visual results. Antigens prepared by N-butanol proved to be valuable diagnostic markers of leptospirosis.
Formaldehyde observations of the Perseus Molecular Cloud
Authors: Mahmut Umut , Esimbek Jarken , Baan Willem , Tang Xindi , Zhou Jianjun , Li Dalei , He Yuxin , Tursun Kadirya , Li Jiasheng , Komesh Toktarkhan , Sailanbek Serikbek
Published: January 2024

Large-scale observations of the Perseus Molecular Cloud (MC) with Nanshan 26-m telescope are presented using the 6 cm ortho-H2CO (110–111) transition. As a probe of dense gas at low temperatures, the H2CO absorption extends over the main parts of the Perseus MC. A comparison of the H2CO absorption line with the 12CO and 13CO (J = 1–0) emissions shows that the H2CO and CO are similarly distributed over the Perseus but that H2CO correlates better with 13CO. Comparison with the Herschel-derived H2 column density shows that both the 13CO and H2CO column densities vary linearly with the H2 column density. The main parameters of H2CO absorption line data show a log-normal distribution, which suggests that the strong non-thermal line-broadening results from large-scale supersonic turbulence related to the star formation. Formaldehyde absorption serves well as a tracer of star formation activity and also the H2CO-to-13CO and H2CO-to-H2 abundances systematically trace the star formation activity in the six subregions of Perseus MC. The H2CO abundances anticorrelate with the number of prestellar and protostellar cores and the IRIS 12 μm flux in the six subregions and reveal the star formation history in the Perseus MC.
Kernel-, mean-, and noise-marginalized Gaussian processes for exoplanet transits andH0 inference
Authors: Kroupa Namu , Yallup David , Handley Will , Hobson Michael
Published: January 2024

Using a fully Bayesian approach, Gaussian process regression is extended to include marginalization over the kernel choice and hyperparameters. In addition, Bayesian model comparison via the evidence enables direct kernel comparison. The calculation of the joint posterior was implemented with a transdimensional sampler which simultaneously samples over the discrete kernel choice and their hyperparameters by embedding these in a higher dimensional space, from which samples are taken using nested sampling. Kernel recovery and mean function inference were explored on synthetic data from exoplanet transit light-curve simulations. Subsequently, the method was extended to marginalization over mean functions and noise models and applied to the inference of the present-day Hubble parameter, H0, from real measurements of the Hubble parameter as a function of redshift, derived from the cosmologically model-independent cosmic chronometer and lambda-cold dark matter-dependent baryon acoustic oscillation observations. The inferred H0 values from the cosmic chronometers, baryon acoustic oscillations, and combined data sets are $H_0= 66 \pm 6,\, 67 \pm 10,\, \mathrm{ and}\,69 \pm 6\,\mathrm{km}\, \mathrm{s}^{-1}\, \mathrm{Mpc}^{-1}$, respectively. The kernel posterior of the cosmic chronometers data set prefers a non-stationary linear kernel. Finally, the data sets are shown to be not in tension with ln R = 12.17 ± 0.02.
Gravitational torque in circumbinary discs: global radial oscillations
Authors: Cimerman Nicolas P , Rafikov Roman R
Published: January 2024

Circumbinary discs (CBDs) arise in many astrophysical settings, including young stellar binaries and supermassive black hole binaries. Their structure is mediated by gravitational torques exerted on the disc by the central binary. The spatial distribution of the binary torque density (so-called excitation torque density) in CBDs is known to feature global large-amplitude, quasi-periodic oscillations, which are often interpreted in terms of the local resonant Lindblad torques. Here, we investigate the nature of these torque oscillations using 2D, inviscid hydrodynamic simulations and theoretical calculations. We show that torque oscillations arise due to the gravitational coupling of the binary potential to the density waves launched near the inner cavity and freely propagating out in the disc. We provide analytical predictions for the radial periodicity of the torque density oscillations and verify them with simulations, showing that disc sound speed and the multiplicity of the density wave spiral arms are the key factors setting the radial structure of the oscillations. Resonant Lindblad torques play no direct role in determining the radial structure and periodicity of the torque oscillations and manifest themselves only by driving the density waves in the disc. We also observe the formation of vortices at the inner edge of the disc, which can provide a non-trivial contribution to the angular momentum transport in the CBD and may be involved in the development of a non-axisymmetric central cavity.
NIR spectral classification of the companion in the gamma-ray binary HESS J1832−093 as an O6 V star
Authors: van Soelen B , Bordas P , Negueruela I , de Oña Wilhelmi E , Papitto A , Ribó M
Published: January 2024

HESS J1832−093 is a member of the rare class of gamma-ray binaries, as recently confirmed by the detection of orbitally modulated X-ray and gamma-ray emission with a period of ∼86 d. The spectral type of the massive companion star has been difficult to retrieve as there is no optical counterpart, but the system is coincident with a near-infrared source. Previous results have shown that the infrared counterpart is consistent with an O or B-type star, but a clear classification is still lacking. We observed the counterpart twice, in 2019 and 2021, with the X-Shooter spectrograph operating on the Very Large Telescope (VLT). The obtained spectra classify the counterpart as an O6 V-type star. We estimate a distance to the source of 6.7 ± 0.5 kpc, although this estimate can be severely affected by the high extinction towards the source. This new O6 V classification for the companion star in HESS J1832−093 provides further support to an apparent grouping around a given spectral type for all discovered gamma-ray binaries that contain an O-type star. This may be due to the interplay between the initial mass function and the wind momentum–luminosity relation.
Osmoregulatory physiology of the angel wing bivalve Cyrtopleura costata (Pholadidae), an estuarine intertidal burrowing mollusc
Authors: Stakowian Nicole , Freire Carolina A
Published: January 2024

Cyrtopleura costata is a pholadid bivalve that inhabits tidal flats inside burrows c. 40 cm deep, and its visceral mass outgrows its shell significantly. Thus, unlike other bivalves, it cannot isolate its soft tissues from the external environment through complete valve closure. One can thus hypothesize that this bivalve would be inevitably more exposed and susceptible to the tidal changes in salinity. No information regarding its osmoregulatory physiology has been found in the literature. We thus investigated how its fluids would respond to seawater dilution and seawater concentration, and how its tissues would be able to keep their moisture content. Individuals of C. costata were acclimated to 30‰ and then independently exposed stepwise (1 h for each 5‰ change) to 25, 20, 15 and 10‰ or to 35, 40, 45 and 50‰. Isolated tissue slices obtained from control animals (30‰) of siphon, mantle and muscle were exposed in vitro to salines corresponding to salinities of 10, 30 and 50‰. Cyrtopleura costata responded as a typical euryhaline osmoconformer between 25 and 40‰, with haemolymph and mantle cavity water being isosmotic to the external medium. However, some ‘buffering’ of haemolymph osmolality was displayed in the most extreme salinities, 10–20‰ and 45–50‰. This could result from muscle contraction and other cellular responses, reducing ‘apparent permeability’ and diffusive fluxes; the muscle was the tissue with the highest capacity to maintain constant hydration despite significant osmotic challenge. Both in vivo and in vitro experiments indicated a higher tolerance of salinity increases than decreases. Thus, if global and local changes in climate and precipitation rates result in more frequent seawater dilution in its habitat, the area occupied by its realized niche may be reduced.
Painful snapping of the middle finger caused by hyperplasia of the ulnar lateral band: A case report
Authors: Matsuo Anna , Suzuki Taku , Kimura Hiroo , Matsumura Noboru , Iwamoto Takuji , Nakamura Masaya
Published: January 2024

Snapping of fingers can be caused by pathologies such as stenosing flexor tenosynovitis. However, snapping symptoms in the metacarpophalangeal (MP) joint caused by hypertension and hyperplasia of the lateral band are rare. We present a 26-year-old female with symptoms of painful snapping of the middle finger. When the finger was actively flexed from the hyperextension of the MP joint, the ulnar lateral band was prominent, and a snapping phenomenon occurred. The cause of the snapping finger was considered to be tightness of the ulnar lateral band, and surgery was planned. Intraoperatively, the ulnar lateral band was tense and hyperplastic. The snapping phenomenon disappeared immediately after the resection of the lateral band. It is important to consider this condition as one of the differential diagnoses of snapping finger when the patient complains of an atypical snapping phenomenon.
Social behavior among nocturnally migrating birds revealed by automated moonwatching
Authors: Bridge Eli S , Honeycutt Wesley T , Chen Angela J , Miller Riley , Kelly Jeffrey F
Published: January 2024

Migrating birds often fly in group formations during the daytime, whereas at night, it is generally presumed that they fly singly. However, it is difficult to quantify group behavior during nocturnal migration as there are few means of directly observing interactions among individuals. We employed an automated form of moonwatching to estimate percentages of birds that appear to migrate in groups during the night within the Central Flyway of North America. We compared percentages of birds in groups across the spring and fall and examined overnight temporal patterns of group behavior. We found groups were rare in both seasons, never exceeding 10% of birds observed, and were almost nonexistent during the fall. We also observed an overnight pattern of group behavior in the spring wherein groups were more commonly detected early in the night and again just before migration activity ceased. This finding may be related to changes in species composition of migrants throughout the night, or alternatively, it suggests that group formation may be associated with flocking activity on the ground as groups are most prevalent when birds begin and end a night of migration.
Transcription factor LBD16 targets cell wall modification/ion transport genes in peach lateral root formation
Authors: Wu Xuelian , Wang Zhe , Du Anqi , Gao Huaifeng , Liang Jiahui , Yu Wenying , Yu Haixiang , Fan Shihao , Chen Qiuju , Guo Jian , Xiao Yuansong , Peng Futian
Published: January 2024

LATERAL ORGAN BOUNDARIES DOMAIN/ASYMMETRIC LEAVES2-LIKEs (LBDs/ASLs) are plant-specific transcription factors that function downstream of auxin-regulated lateral root (LR) formation. Our previous research found that PpLBD16 positively regulates peach (Prunus persica) LR formation. However, the downstream regulatory network and target genes of PpLBD16 are still largely unknown. Here, we constructed a PpLBD16 homologous overexpression line and a PpLBD16 silenced line. We found that overexpressing PpLBD16 promoted peach root initiation, while silencing PpLBD16 inhibited peach root formation. Through RNA sequencing (RNA-seq) analysis of roots from PpLBD16 overexpression and silenced lines, we discovered that genes positively regulated by PpLBD16 were closely related to cell wall synthesis and degradation, ion/substance transport, and ion binding and homeostasis. To further detect the binding motifs and potential target genes of PpLBD16, we performed DNA-affinity purification sequencing (DAP-seq) analysis in vitro. PpLBD16 preferentially bound to CCNGAAANNNNGG (MEME-1), [C/T]TTCT[C/T][T/C] (MEME-2), and GCGGCGG (ABR1) motifs. By combined analysis of RNA-seq and DAP-seq data, we screened candidate target genes for PpLBD16. We demonstrated that PpLBD16 bound and activated the cell wall modification-related genes EXPANSIN-B2 (PpEXPB2) and SUBTILISIN-LIKE PROTEASE 1.7 (PpSBT1.7), the ion transport-related gene CYCLIC NUCLEOTIDE-GATED ION CHANNEL 1 (PpCNGC1) and the polyphenol oxidase (PPO)-encoding gene PpPPO, thereby controlling peach root organogenesis and promoting LR formation. Moreover, our results displayed that PpLBD16 and its target genes are involved in peach LR primordia development. Overall, this work reveals the downstream regulatory network and target genes of PpLBD16, providing insights into the molecular network of LBD16-mediated LR development.
Mechanical advantage makes stomatal opening speed a function of evaporative demand
Authors: Pichaco Javier , Manandhar Anju , McAdam Scott A M
Published: January 2024

Stomatal opening in the light, observed in nearly all vascular land plants, is essential for providing access to atmospheric CO2 for photosynthesis. The speed of stomatal opening in the light is critical for maximizing carbon gain in environments in which light intensity changes, yet we have little understanding of how other environmental signals, particularly evaporative demand driven by vapor pressure deficit (VPD) influences the kinetics of this response. In angiosperms, and some fern species from the family Marsileaceae, a mechanical interaction between the guard cells and the epidermal cells determines the aperture of the pore. Here, we examine whether this mechanical interaction influences the speed of stomatal opening in the light. To test this, we investigated the speed of stomatal opening in response to light across a range of VPDs in seven plant species spanning the evolutionary diversity of guard cell and epidermal cell mechanical interactions. We found that stomatal opening speed is a function of evaporative demand in angiosperm species and Marsilea, which have guard cell and epidermal cell mechanical interactions. Stomatal opening speeds did not change across a range of VPD in species of gymnosperm and fern, which do not have guard cell mechanical interactions with the epidermis. We find that guard cell and epidermal cell mechanical interactions may play a key role in regulating stomatal responsiveness to light. These results provide valuable insight into the adaptive relevance of mechanical advantage.
What can multiple causes of death tell about cardiovascular mortality during COVID-19 pandemic in the United States?
Authors: Ukolova Elizaveta , Burcin Boris
Published: January 2024

Background

The COVID-19 pandemic may have caused an underestimation of cardiovascular disease (CVD) mortality, as COVID-19 was predominantly recorded as the underlying cause of death. This study investigates CVD-related excess mortality and recording of CVD on the death certificates during 2020–2021, considering underlying (underlying causes of death (UCD)), immediate and contributory causes.

Methods

We utilize US Multiple-Cause-of-Death Mortality Data. Excess deaths are assessed by comparing actual 2020–2021 deaths with Seasonal Autoregressive Integrated Moving Average model predictions. To understand changes in cause-of-death recording, we use the standardized ratio of multiple to underlying causes (SRMU).

Results

Excess CVD mortality is most prominent in contributory causes, including hypertensive disease, essential hypertension, and acute myocardial infarction. While excess of contributory CVDs generally decreased in 2021, acute myocardial infarction, pulmonary heart diseases and other circulatory diseases showed a continual increase. Changes in SRMU from 2020 to 2021, compared to 2010–2019, reveal shifts in coding practices, particularly for pulmonary heart, cerebrovascular diseases, non-rheumatic valve disorders and heart failure.

Conclusions

The COVID-19 pandemic has significantly increased CVD-related mortality, which is not fully captured in conventional analyses based solely on the UCD. The trend of coding CVDs as non-underlying causes of death accelerated during 2020–2021. Multiple-causes-of-death should be employed to evaluate mortality when new leading cause of death emerges.
Lupus enteritis: a narrative review
Authors: Muñoz-Urbano Marcela , Sangle Shirish , D’Cruz David P
Published: January 2024

Lupus enteritis (LE) is a rare manifestation of systemic lupus erythematosus. The pathophysiology of LE has not been fully elucidated, although inflammatory and thrombotic processes are likely important factors. The underlying pathophysiological mechanisms may depend on which portion of the intestine is affected. Over half of the patients with LE also present with renal or haematological complications. The diagnosis of LE is based on clinical, histopathological and imaging findings; abdominal computed tomography (CT) is the gold standard in diagnosis. Abdominal CT can also identify factors that predict complications and could potentially guide pharmacological and nutritional management. Timely identification and prompt treatment initiation are paramount to avoid life and organ threatening complications. Glucocorticoids are often the first-line treatment. Additional therapy including immunosuppressive therapy is utilised on a case-by-case basis as there are no clinical trials to define the optimal therapeutic approach. Surgical intervention may be needed especially if there is bowel perforation or peritonitis. In general, the prognosis of LE is good.