bluuebunny/crossref_metadata_2025 huggingface dataset:
Gray Matter Volume and Functional Connectivity in Hypochondriasis: A Magnetic Resonance Imaging and Support Vector Machine Analysis
Authors: Shen Zhe , Yu Liang , Zhao Zhiyong , Jin Kangyu , Pan Fen , Hu Shaohua , Li Shangda , Xu Yi , Xu Dongrong , Huang Manli
Published: December 2020

Objective: Patients with hypochondriasis hold unexplainable beliefs and a fear of having a lethal disease, with poor compliances and treatment response to psychotropic drugs. Although several studies have demonstrated that patients with hypochondriasis demonstrate abnormalities in brain structure and function, gray matter volume (GMV) and functional connectivity (FC) in hypochondriasis still remain unclear.

Methods: The present study collected T1-weighted and resting-state functional magnetic resonance images from 21 hypochondriasis patients and 22 well-matched healthy controls (HCs). We first analyzed the difference in the GMV between the two groups. We then used the regions showing a difference in GMV between two groups as seeds to perform functional connectivity (FC) analysis. Finally, a support vector machine (SVM) was applied to the imaging data to distinguish hypochondriasis patients from HCs.

Results: Compared with the HCs, the hypochondriasis group showed decreased GMV in the left precuneus, and increased GMV in the left medial frontal gyrus. FC analyses revealed decreased FC between the left medial frontal gyrus and cuneus, and between the left precuneus and cuneus. A combination of both GMV and FC in the left precuneus, medial frontal gyrus, and cuneus was able to discriminate the hypochondriasis patients from HCs with a sensitivity of 0.98, specificity of 0.93, and accuracy of 0.95.

Conclusion: Our study suggests that smaller left precuneus volumes and decreased FC between the left precuneus and cuneus seem to play an important role of hypochondriasis. Future studies are needed to confirm whether this finding is generalizable to patients with hypochondriasis.
Fetal Cortical Plate Segmentation Using Fully Convolutional Networks With Multiple Plane Aggregation
Authors: Hong Jinwoo , Yun Hyuk Jin , Park Gilsoon , Kim Seonggyu , Laurentys Cynthia T. , Siqueira Leticia C. , Tarui Tomo , Rollins Caitlin K. , Ortinau Cynthia M. , Grant P. Ellen , Lee Jong-Min , Im Kiho
Published: December 2020

Fetal magnetic resonance imaging (MRI) has the potential to advance our understanding of human brain development by providing quantitative information of cortical plate (CP) development in vivo. However, for a reliable quantitative analysis of cortical volume and sulcal folding, accurate and automated segmentation of the CP is crucial. In this study, we propose a fully convolutional neural network for the automatic segmentation of the CP. We developed a novel hybrid loss function to improve the segmentation accuracy and adopted multi-view (axial, coronal, and sagittal) aggregation with a test-time augmentation method to reduce errors using three-dimensional (3D) information and multiple predictions. We evaluated our proposed method using the ten-fold cross-validation of 52 fetal brain MR images (22.9–31.4 weeks of gestation). The proposed method obtained Dice coefficients of 0.907 ± 0.027 and 0.906 ± 0.031 as well as a mean surface distance error of 0.182 ± 0.058 mm and 0.185 ± 0.069 mm for the left and right, respectively. In addition, the left and right CP volumes, surface area, and global mean curvature generated by automatic segmentation showed a high correlation with the values generated by manual segmentation (R2 > 0.941). We also demonstrated that the proposed hybrid loss function and the combination of multi-view aggregation and test-time augmentation significantly improved the CP segmentation accuracy. Our proposed segmentation method will be useful for the automatic and reliable quantification of the cortical structure in the fetal brain.
Influence of Different LED Light Colour Temperatures on the Preference Behaviour of Weaned Piglets
Authors: Götz Sven , Raoult Camille M. C. , Reiter Klaus , Wensch-Dorendorf Monika , Werner Daniel , von Borell Eberhard
Published: December 2020

This study investigates the effect of different LED lighting colour temperatures on the preference behaviour of 4-week-old weaned piglets. A total of 32 piglets were housed in two replications in an experimental pen area with four identically equipped pen compartments connected two by two. Each pen unit offered a compartment set to a colour temperature of 3000 kelvin and another set to 6500 kelvin, at 80 lux during the day. Each piglet could freely choose between the two compartments by using a passageway. Over a period of five weeks, the behaviours “lying”, “eating” and “activity” were video recorded for 72 h during the 1st, 3rd and 5th week of the experiment. The location of the piglet in the pen and its behaviour were determined by using time sampling. In the first week, the piglets preferred the colour temperature of 3000 K to perform all behaviours. In the following weeks this preference decreased. Results also show that feed consumption and soiling of the pens were higher under 6500 K. Pigs can differentiate between the different colour temperatures and use them for different behaviours. This can be used to divide pens into functional areas in order to better suit the behavioural needs of pigs.
Effects of High Toughness and Welding Residual Stress for Unstable Fracture Prevention
Authors: An Gyubaek , Park Jeongung , Han Ilwook
Published: December 2020

Unstable fractures generally occur in brittle materials under low-temperature service conditions. Toughness and welding residual stress are the main factors that should be evaluated when defining a brittle crack propagation path. In this study, a rainbow welding technique was proposed and confirmed as being significantly useful in preventing unstable fractures in weld joints. The residual compressive stress in the crack front was particularly useful for decreasing the possibility of brittle fracture. The objective was to examine the effect of high welding consumable toughness welding residual stress, especially for avoiding brittle fracture through welding residual compressive stress.
Polyphenols Extracted from Chinese Hickory (Carya cathayensis) Promote Apoptosis and Inhibit Proliferation through the p53-Dependent Intrinsic and HIF-1α-VEGF Pathways in Ovarian Cancer Cells
Authors: He Zhiping , Wu Shaozhen , Lin Ju , Booth Ashley , Rankin Gary O’Neal , Martinez Ivan , Chen Yi Charlie
Published: December 2020

Ovarian cancer is the second most common gynecologic cancer with an estimated 13,940 mortalities across the United States in 2020. Natural polyphenols have been shown to double the survival time of some cancer patients due to their anticancer properties. Therefore, the effect of polyphenols extracted from Chinese hickory seed skin Carya cathayensis (CHSP) on ovarian cancer was investigated in the present study. Cell viability results showed that CHSP is more effective in inhibiting ovarian cancer cells than normal ovarian cells, with the IC50 value for inhibition of cell proliferation of Ovarian cancer cells (OVCAR-3) being 10.33 ± 0.166 µg/mL for a 24 h treatment. Flow cytometry results showed that the apoptosis rate was significantly increased to 44.21% after 24 h treatment with 20 µg/mL of CHSP. Western blot analysis showed that CHSP induced apoptosis of ovarian cancer cells through a p53-dependent intrinsic pathway. Compared with control values, levels of VEGF excreted by OVCAR-3 cancer cells were reduced to 7.87% with a 40 µg/mL CHSP treatment. Consistent with our previous reports, CHSP inhibits vascular endothelial growth factor (VEGF) secretion by regulating the HIF-1α-VEGF pathway. In addition, we also found that the inhibitory effect of CHSP on ovarian cancer is related to the up-regulation of Phosphatase and tension homolog (PTEN) and down-regulation of nuclear factor kappa-B (NF-kappa B). These findings provide some evidence of the anti-ovarian cancer properties of CHSP and support the polyphenols as potential candidates for ovarian cancer adjuvant therapy.
The Foreign Oligochaete Species Quistadrilus multisetosus (Smith, 1900) in Lake Geneva: Morphological and Molecular Characterization and Environmental Influences on Its Distribution
Authors: Vivien Régis , Lafont Michel , Lods-Crozet Brigitte , Holzmann Maria , Apothéloz-Perret-Gentil Laure , Guigoz Yaniss , Ferrari Benoit J. D.
Published: December 2020

The presence of the oligochaete species Quistadrilus multisetosus (Smith, 1900) originating from North America has been mentioned for several decades in Europe, the Middle East and Russia. Its distribution and abundance in Europe is still unknown but it can be considered as potentially invasive. This species was recently discovered in Lake Geneva (Switzerland/France) and three other Swiss lakes. The aims of the present work are to report its repartition and abundance in Lake Geneva, to study its ecology and to determine its invasive potential in this lake. We also provide an identification key for correctly differentiating Q. multisetosus from the closely related species Spirosperma ferox Eisen, 1879 and Embolocephalus velutinus (Grube, 1879), and study the phylogenetic position of Q. multisetosus within several Tubificinae lineages based on the cytochrome c oxidase (COI) marker. Twenty-eight sites have been monitored since 2009 in Lake Geneva. In several sites, the COI sequence corresponding to this species was also searched for in sediment samples using high-throughput sequencing. In addition, we examined specimens collected in this lake before 2009 likely to belong to Q. multisetosus and to have been misidentified. We found that Q. multisetosus was only present in the lake downstream of a wastewater treatment plant and a combined sewer overflow in the Vidy Bay (near Lausanne) and at a site located nearby. These results confirmed the high tolerance of this species to organic matter pollution. Q. multisetosus was already present in this location in 1974 (misidentified as Spirosperma ferox), which suggests that Q. multisetosus has a limited capacity to disseminate in this lake. However, we recommend continuing monitoring its presence in Lake Geneva in the future, especially in the context of warming of waters that could contribute to the expansion of this species.
New Trends for Antimalarial Drugs: Synergism between Antineoplastics and Antimalarials on Breast Cancer Cells
Authors: Duarte Diana , Vale Nuno
Published: December 2020

Chemotherapy plays a key role in breast cancer therapy, but drug resistance and unwanted side effects make the treatment less effective. We propose a new combination model that combines antineoplastic drugs and antimalarials for breast cancer therapy. Cytotoxic effects of two antineoplastic agents alone and in combination with several antimalarials on MCF-7 tumor cell line was evaluated. Different concentrations in a fixed ratio were added to the cultured cells and incubated for 48 h. Cell viability was evaluated using MTT and SRB assays. Synergism was evaluated using the Chou-Talalay method. The results indicate doxorubicin (DOX) and paclitaxel (PTX) alone at concentrations of their IC50 and higher are cell growth inhibitors. Mefloquine, artesunate, and chloroquine at concentrations of their IC50 demonstrate anti-cancer activity. In combination, almost all antimalarials demonstrate higher ability than DOX and PTX alone to decrease cell viability at concentrations of IC50 and lower than their IC50. The combination of chloroquine, artesunate and mefloquine with DOX and PTX was synergic (CI < 1). The combination of DOX and mefloquine after 48 h incubation demonstrated the highest cytotoxicity against MCF-7 cells, and the combination of DOX and artesunate was the most synergic. These results suggest antimalarials could act synergistically with DOX/PTX for breast cancer therapy.
Recent Development of Aminoacyl-tRNA Synthetase Inhibitors for Human Diseases: A Future Perspective
Authors: Kim Soong-Hyun , Bae Seri , Song Minsoo
Published: December 2020

Aminoacyl-tRNA synthetases (ARSs) are essential enzymes that ligate amino acids to tRNAs and translate the genetic code during protein synthesis. Their function in pathogen-derived infectious diseases has been well established, which has led to the development of small molecule therapeutics. The applicability of ARS inhibitors for other human diseases, such as fibrosis, has recently been explored in the clinical setting. There are active studies to find small molecule therapeutics for cancers. Studies on central nervous system (CNS) disorders are burgeoning as well. In this regard, we present a concise analysis of the recent development of ARS inhibitors based on small molecules from the discovery research stage to clinical studies as well as a recent patent analysis from the medicinal chemistry point of view.
Biochemical Pathways Leading to the Formation of Wyosine Derivatives in tRNA of Archaea
Authors: Urbonavičius Jaunius , Tauraitė Daiva
Published: December 2020

Tricyclic wyosine derivatives are present at position 37 in tRNAPhe of both eukaryotes and archaea. In eukaryotes, five different enzymes are needed to form a final product, wybutosine (yW). In archaea, 4-demethylwyosine (imG-14) is an intermediate for the formation of three different wyosine derivatives, yW-72, imG, and mimG. In this review, current knowledge regarding the archaeal enzymes involved in this process and their reaction mechanisms are summarized. The experiments aimed to elucidate missing steps in biosynthesis pathways leading to the formation of wyosine derivatives are suggested. In addition, the chemical synthesis pathways of archaeal wyosine nucleosides are discussed, and the scheme for the formation of yW-86 and yW-72 is proposed. Recent data demonstrating that wyosine derivatives are present in the other tRNA species than those specific for phenylalanine are discussed.
Abemaciclib, A Selective CDK4/6 Inhibitor, Restricts the Growth of Pediatric Ependymomas
Authors: Liang Muh-Lii , Chen Chun-Han , Liu Yun-Ru , Huang Man-Hsu , Lin Yu-Chen , Wong Tai-Tong , Lin Sey-En , Chu Shing-Shiung , Ding Yi-Huei , Hsieh Tsung-Han
Published: December 2020

Pediatric ependymomas are a type of malignant brain tumor that occurs in children. The overall 10-year survival rate has been reported as being 45–75%. Maximal safe surgical resection combined with adjuvant chemoradiation therapy is associated with the highest overall and progression-free survival rates. Despite aggressive treatment, one-third of ependymomas exhibit recurrence within 2 years of initial treatment. Therefore, this study aimed to find new agents to overcome chemoresistance and defer radiotherapy treatment since, in addition, radiation exposure may cause long-term side effects in the developing brains of young children. By using integrated bioinformatics and through experimental validation, we found that at least one of the genes CCND1 and CDK4 is overexpressed in ependymomas. The use of abemaciclib, a highly selective CDK4/6 inhibitor, effectively inhibited cell proliferation and reduced the expression of cell-cycle-related and DNA-repair-related gene expression via the suppression of RB phosphorylation, which was determined through RNA-seq and Western blot analyses. Furthermore, abemaciclib effectively induced cell death in vitro. The efficiency of abemaciclib was validated in vivo using subcutaneously implanted ependymoma tissues from patient-derived xenografts (PDXs) in mouse models. Treatment with abemaciclib showed encouraging results in preclinical pediatric ependymoma models and represents a potential therapeutic strategy for treating challenging tumors in children.
Organ Preservation after Endoscopic Resection of Early Esophageal Cancer with a High Risk of Lymph Node Involvement
Authors: Dermine Solène , Lévi-Strauss Thomas , Abou Ali Einas , Belle Arthur , Leblanc Sarah , Bibault Jean-Emmanuel , Barré Amélie , Palmieri Lola-Jade , Brezault Catherine , Dhooge Marion , Terris Benoit , Dohan Anthony , Soyer Philippe , Berger Arthur , Rahmi Gabriel , Coriat Romain , Chaussade Stanislas , Barret Maximilien
Published: December 2020

Background: Esophagectomy is recommended after endoscopic resection of an early esophageal cancer when pejorative histoprognostic criteria indicate a high risk of lymph node involvement. Our aim was to analyze the clinical outcomes of a non-surgical, organ preserving management in this clinical setting. Patients and Methods: This retrospective study was performed in two tertiary centers from 2015 to 2020. Patients were included if they had histologically complete resection of an early esophageal cancer, with poor differentiation, lymphovascular invasion or deep submucosal invasion. Endoscopic resection was followed by chemoradiotherapy or follow-up in case of surgical contraindications or patient refusal. Outcome measures were disease-free survival (DFS), overall survival (OS), cancer specific survival (CSS) and toxicity of chemoradiotherapy. Results: Forty-one patients (36 with squamous cell carcinoma and 5 with adenocarcinomas) were included. The estimated high risk of lymph node involvement was based on poor differentiation (10/41; 24%), lympho-vascular invasion (11/41; 27%), muscularis mucosa invasion or deep sub-mucosal invasion (38/41; 93%). Thirteen patients (13/41; 32%) were closely monitored, and 28 (28/41; 68%) were treated by chemoradiotherapy or radiotherapy alone. In the close follow-up group, DFS, OS and CSS were 92%, 92% and 100%, respectively vs. 75%, 79% and 96%, respectively in the chemoradiotherapy group at the end of the follow-up. Serious adverse events related to chemoradiotherapy occurred in 10% of the patients. There were no treatment-related deaths. Conclusions: Our study shows that close follow-up may be an alternative to systematic esophagectomy after endoscopic resection of early esophageal cancer with a predicted high risk of lymph node involvement.
Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures
Authors: Suelzu Caterina M. , Conti Virna , Khalidy Youssef , Montagna Sara , Strusi Gabriele , Di Lecce Rosanna , Berni Priscilla , Basini Giuseppina , Ramoni Roberto , Grolli Stefano
Published: December 2020

Mesenchymal stem cells (MSCs) have been recently introduced in veterinary medicine as a potential therapeutic tool for several pathologies. The large-scale in vitro expansion needed to ensure the preparation of a suitable number of MSCs for clinical application usually requires the use of xenogeneic supplements like the fetal bovine serum (FBS). The substitution of FBS with species-specific supplements would improve the safety of implanted cells, reducing the risk of undesired immune responses following cell therapy. We have evaluated the effectiveness of canine adipose tissue-derived stromal vascular fraction (SVF) and MSCs (ADMSCs) expansion in the presence of canine blood-derived supplements. Cells were cultured on traditional plastic surface and inside a 3D environment derived from the jellification of different blood-derived products, i.e., platelet-poor plasma (PPP), platelet-rich plasma (PRP), or platelet lysate (PL). PPP, PRP, and PL can contribute to canine ADMSCs in vitro expansion. Both allogeneic and autologous PPP and PL can replace FBS for ADMSCs culture on a plastic surface, exhibiting either a similar (PPP) or a more effective (PL) stimulus to cell replication. Furthermore, the 3D environment based on homospecific blood-derived products polymerization provides a strong stimulus to ADMSCs replication, producing a higher number of cells in comparison to the plastic surface environment. Allogeneic or autologous blood products behave similarly. The work suggests that canine ADMSCs can be expanded in the absence of xenogeneic supplements, thus increasing the safety of cellular preparations. Furthermore, the 3D fibrin-based matrices could represent a simple, readily available environments for effective in vitro expansion of ADMSCs using allogeneic or autologous blood-products.
Graph Reachability on Parallel Many-Core Architectures
Authors: Quer Stefano , Calabrese Andrea
Published: December 2020

Many modern applications are modeled using graphs of some kind. Given a graph, reachability, that is, discovering whether there is a path between two given nodes, is a fundamental problem as well as one of the most important steps of many other algorithms. The rapid accumulation of very large graphs (up to tens of millions of vertices and edges) from a diversity of disciplines demand efficient and scalable solutions to the reachability problem. General-purpose computing has been successfully used on Graphics Processing Units (GPUs) to parallelize algorithms that present a high degree of regularity. In this paper, we extend the applicability of GPU processing to graph-based manipulation, by re-designing a simple but efficient state-of-the-art graph-labeling method, namely the GRAIL (Graph Reachability Indexing via RAndomized Interval) algorithm, to many-core CUDA-based GPUs. This algorithm firstly generates a label for each vertex of the graph, then it exploits these labels to answer reachability queries. Unfortunately, the original algorithm executes a sequence of depth-first visits which are intrinsically recursive and cannot be efficiently implemented on parallel systems. For that reason, we design an alternative approach in which a sequence of breadth-first visits substitute the original depth-first traversal to generate the labeling, and in which a high number of concurrent visits is exploited during query evaluation. The paper describes our strategy to re-design these steps, the difficulties we encountered to implement them, and the solutions adopted to overcome the main inefficiencies. To prove the validity of our approach, we compare (in terms of time and memory requirements) our GPU-based approach with the original sequential CPU-based tool. Finally, we report some hints on how to conduct further research in the area.
Instability in In0.7Ga0.3As Quantum-Well MOSFETs with Single-Layer Al2O3 and Bi-Layer Al2O3/HfO2 Gate Stacks Caused by Charge Trapping under Positive Bias Temperature (PBT) Stress
Authors: Kwon Hyuk-Min , Kim Dae-Hyun , Kim Tae-Woo
Published: December 2020

The instability of transistor characteristics caused by charge trapping under positive bias temperature (PBT) stress in In0.7Ga0.3As metal oxide semiconductor field-effect transistors (MOSFETs) with single-layer Al2O3 and bi-layer Al2O3/HfO2 gate stacks was investigated. The equivalent field across the multi-gate stacks was compared with a single layer used to compare the instability of electrical characteristics. The observed threshold voltage degradation (ΔVT) was consistent with the phenomenon of fast transient trapping of injected electrons at pre-existing shallow defects in the high-κ dielectric of HfO2, in which this charging was recovered by applying a relaxation voltage. Excluding the fast-transient charging components, the power law exponent (n), with respect to the time-dependent threshold voltage degradation, ranged from 0.3 to 0.35 for both single-layer Al2O3 and bi-layer Al2O3/HfO2 gate stacks. This long-term (slow) VT shift, which was strongly correlated with transconductance (Gm) degradation, was attributed to significant charge trapping in the border trap or/and defect sites within the high-κ dielectric.
Smart Contract Engineering
Authors: Hu Kai , Zhu Jian , Ding Yi , Bai Xiaomin , Huang Jiehua
Published: December 2020

A smart contract is the algorithmic description of a contractual transaction protocol that is automatically executed together with the information provided by its parties. It is written in a simplified programming language that is specific to a particular domain. Not only correctness and unambiguity are its essential formal properties, but also conformance to any legislation governing the matter of the transaction. Finally, and importantly, the trustworthiness, safety and security of the platform executing the transactions are its main attributes. An emerging challenge is to define a proper engineering process to meet the demanding requirements while supporting mass production and distribution. This paper proposes the concept of smart contract engineering (SCE) to facilitate the generation of smart legal contracts, which is the combination of software engineering, formal methods and computational law. SCE aims to reduce the potential errors and improve efficiency during the contract development process, meanwhile promote the standardization of contract design methodologies. In this paper, the roadmap of an iterative refinement-based, model-driven formal design methodology is introduced, not only to validate smart contracts but also to support the whole life cycle of their engineering.
The Enhancement of Weak Bearing Fault Signatures by Stochastic Resonance with a Novel Potential Function
Authors: Zhang Chao , Duan Haoran , Xue Yu , Zhang Biao , Fan Bin , Wang Jianguo , Gu Fengshou
Published: December 2020

As the critical parts of wind turbines, rolling bearings are prone to faults due to the extreme operating conditions. To avoid the influence of the faults on wind turbine performance and asset damages, many methods have been developed to monitor the health of bearings by accurately analyzing their vibration signals. Stochastic resonance (SR)-based signal enhancement is one of effective methods to extract the characteristic frequencies of weak fault signals. This paper constructs a new SR model, which is established based on the joint properties of both Power Function Type Single-Well and Woods-Saxon (PWS), and used to make fault frequency easy to detect. However, the collected vibration signals usually contain strong noise interference, which leads to poor effect when using the SR analysis method alone. Therefore, this paper combines the Fourier Decomposition Method (FDM) and SR to improve the detection accuracy of bearing fault signals feature. Here, the FDM is an alternative method of empirical mode decomposition (EMD), which is widely used in nonlinear signal analysis to eliminate the interference of low-frequency coupled signals. In this paper, a new stochastic resonance model (PWS) is constructed and combined with FDM to enhance the vibration signals of the input and output shaft of the wind turbine gearbox bearing, make the bearing fault signals can be easily detected. The results show that the combination of the two methods can detect the frequency of a bearing failure, thereby reminding maintenance personnel to urgently develop a maintenance plan.
Evaluation of Demand Response Potential Flexibility in the Industry Based on a Data-Driven Approach
Authors: Lee Eunjung , Baek Keon , Kim Jinho
Published: December 2020

The rapid increase in renewable energy resources has resulted in the increasing need for a demand flexibility program (DFP) from industrial load resources as a solution to oversupply and peak load spikes. However, to reasonably estimate the DR potential flexibility, the load characteristics must be analyzed and potential assessment formulas must be validated. Thus, in this study, a novel method is proposed to evaluate the DR potential flexibility of industrial loads according to a process of related load-characteristic data analysis. The proposed potential-estimation model considers frequency, consistency, and DR event operation scores during designated ramp-up and ramp-down time intervals separately. A case study was conducted by considering typical cement industry process with actual power-consumption data analysis for demonstrating the test system. The results confirm that load reduction of more than half of the usual power consumption is possible if a potential score is about 0.27 in cement industry cases. Thus, the proposed method can be used as an indicator to determine how an industrial load is adequate for obtaining a DFP while suggesting meaningful implications through industrial load-resource data analysis.
NCA, NCM811, and the Route to Ni-Richer Lithium-Ion Batteries
Authors: Julien Christian M. , Mauger Alain
Published: December 2020

The aim of this article is to examine the progress achieved in the recent years on two advanced cathode materials for EV Li-ion batteries, namely Ni-rich layered oxides LiNi0.8Co0.15Al0.05O2 (NCA) and LiNi0.8Co0.1Mn0.1O2 (NCM811). Both materials have the common layered (two-dimensional) crystal network isostructural with LiCoO2. The performance of these electrode materials are examined, the mitigation of their drawbacks (i.e., antisite defects, microcracks, surface side reactions) are discussed, together with the prospect on a next generation of Li-ion batteries with Co-free Ni-rich Li-ion batteries.
Failure Analysis of a Pre-Excavation Double Equipment Withdrawal Channel and Its Control Techniques
Authors: Li Chen , Guo Xiaofei , Lian Xiaoyong , Ma Nianjie
Published: December 2020

The use of pre-excavation equipment withdrawal channels (EWCs) at the stop-production line is important for the rapid withdrawal of coal mining equipment. However, during the final mining period, the dynamic pressure of a pre-excavated double EWC is severe, which leads to instability of the surrounding rock around the EWCs. Therefore, in this paper, the methods of field monitoring, theoretical analysis, and numerical simulation were used to systematically study the stress and plastic zone evolution of a double EWC during the final mining period. Firstly, the distribution characteristics of mining abutment pressure and roadway failure modes under the action of mining abutment pressure were analyzed theoretically. Afterward, a FLAC3D mining numerical model was established according to the distribution of rock strata obtained from roof detection. Finally, the evolution laws of the stress fields and plastic zones of the EWCs during final mining were obtained by numerical simulation. The present study suggests that asymmetric stress distribution dominates asymmetric failure of the surrounding rock around the EWCs during the final mining period, and deformation failure within 10 m from the working face to the main EWC (MEWC) accounted for most of the roadway deformation. Based on the research results combined with actual production experience, the stability control technique of the surrounding rock with reinforcement of anchor cables and double-row buttress hydraulic support for the MEWC was put forward. After the field application, the ideal result was obtained.
Investigation of the Geometric Shape Effect on the Solar Energy Potential of Gymnasium Buildings
Authors: Jiang Lei , Liu Weiqing , Liao Haiping , Li Jiabao
Published: December 2020

Gymnasium are typically large-span buildings with abundant solar energy resources due to their extensive roof surface. However, relevant research on this topic has not been thoroughly conducted to investigate the effect of the geometric shape of gymnasium buildings on their solar potential. In this paper, an investigation of the geometric shape effect on the solar potential of gymnasium buildings is presented. A three-dimensional radiation transfer model coupled with historical meteorological data was established to estimate the real-time solar potential of the roof of a gymnasium building. The rooftop solar potential of three typical building foundation shapes and different types of roof shapes that have evolved was systematically analyzed. An annual solar potential cloud map of each gymnasium building is generated. The monthly and annual average solar radiation intensities of the different types of roof shapes are investigated. Compared to the optimal tilt angle, the maximum decrease in the average radiation intensity reached −20.42%, while the minimum decline was −8.64% for all types of building shapes. The solar energy potential fluctuated by up to 11% across the various roof shapes, which indicate that shape selection is of vital importance for integrated photovoltaic gymnasium buildings. The results presented in this work are essential for clarifying the effects of the geometric shape of gymnasium buildings on the solar potential of their roofs, which provide an important reference for building design.
Seasonal Variation in Aflatoxin Levels in Edible Seeds, Estimation of Its Dietary Intake and Vitamin E Levels in Southern Areas of Punjab, Pakistan
Authors: Razis Ahmad Faizal Abdull , Shehzad Muhammed Muzammel , Usman Sunusi , Ali Nada Basheir , Iqbal Shahzad Zafar , Naheed Nadia , Asi Muhammad Rafique
Published: December 2020

A total of 779 samples of edible nuts (melon seeds, watermelon seeds, pumpkin seeds, and cantaloupe seeds) from Southern Punjab (Pakistan), were collected during the summer and the winter seasons. The natural occurrence of aflatoxins (AFs) and vitamin E (tocopherols) levels were investigated using HPLC. The results have shown that 180 (43.4%) of samples from the winter season and 122 (33.4%) samples from the summer season were found positive for AFs. Elevated average levels of total AFs (20.9 ± 3.10 μg/kg, dry weight) were observed in watermelon seeds without shell, and the lowest average amount (15.9 ± 3.60 μg/kg) were documented in melon seeds without shell samples from the winter season. An elevated average amount of total AFs 17.3 ± 1.50 μg/kg was found in pumpkin seeds available without a shell. The results have documented a significant difference in total AFs levels in edible seeds available with shells versus without shells (α = 0.05 & 0.01). The highest dietary intake of 6.30 μg/kg/day was found in female individuals from consuming pumpkin seeds (without shell) in the winter season. A value of 3.00 μg/kg/day was found in pumpkin seed without shell in the summer season in female individuals. The highest total tocopherol levels were 22.2 ± 7.70 ng/100 g in pumpkin seeds samples from the winter season and 14.5 ± 5.50 mg/100 g in melon seed samples from the summer season. The variation of total tocopherol levels in edible seeds among the winter and summer seasons showed a significant difference (p ≤ 0.0054), except watermelon seeds samples with non-significant differences (p ≥ 0.183).
Spatiotemporal LUCC Simulation under Different RCP Scenarios Based on the BPNN_CA_Markov Model: A Case Study of Bamboo Forest in Anji County
Authors: Huang Zihao , Du Huaqiang , Li Xuejian , Zhang Meng , Mao Fangjie , Zhu Di’en , He Shaobai , Liu Hua
Published: December 2020

Simulating spatiotemporal land use and land cover change (LUCC) data precisely under future climate scenarios is an important basis for revealing the carbon cycle response of forest ecosystems to LUCC. In this paper, a coupling model consisting of a back propagation neural network (BPNN), Markov chain, and cellular automata (CA) was designed to simulate the LUCC in Anji County, Zhejiang Province, under four climate scenarios (Representative Concentration Pathway (RCP) 2.6, 4.5, 6.0, 8.5) from 2024 to 2049 and to analyze the temporal and spatial distribution of bamboo forests in Anji County. Our results provide four outcomes. (1) The transition probability matrices indicate that the area of bamboo forests shows an expansion trend, and the largest contribution to the expansion of bamboo forests is the cultivated land. The Markov chain composed of the average transition probability matrix could perform excellently, with only small errors when simulating the areas of different land-use types. (2) Based on the optimized BPNN, which had a strong generalization ability, a high prediction accuracy, and area under the curve (AUC) values above 0.9, we could obtain highly reliable land suitability probabilities. After introducing more driving factors related to bamboo forests, the prediction of bamboo forest changes will be more accurate. (3) The BPNN_CA_Markov coupling model could achieve high-precision simulation of LUCC at different times, with an overall accuracy greater than 70%, and the consistency of the LUCC simulation from one time to another also had good performance, with a figure of merit (FOM) of approximately 40%. (4) Under the future four RCP scenarios, bamboo forest evolution had similar spatial characteristics; that is, bamboo forests were projected to expand in the northeast, south, and southwest mountainous areas of Anji County, while bamboo forests were projected to decline mainly around the junction of the central and mountainous areas of Anji County. Comparing the simulation results of different scenarios demonstrates that 74% of the spatiotemporal evolution of bamboo forests will be influenced by the interactions and competition among different land-use types and other driving factors, and 26% will come from different climate scenarios, among which the RCP8.5 scenario will have the greatest impact on the bamboo forest area and spatiotemporal evolution, while the RCP2.6 scenario will have the smallest impact. In short, this study proposes effective methods and ideas for LUCC simulation in the context of climate change and provides accurate data support for analyzing the impact of LUCC on the carbon cycle of bamboo forests.
Innate Immunity and Biological Therapies for the Treatment of Sjögren’s Syndrome
Authors: Srivastava Amrita , Makarenkova Helen P.
Published: December 2020

Sjögren’s syndrome (SS) is a systemic autoimmune disorder affecting approximately 3% of the population in the United States. This disease has a female predilection and affects exocrine glands, including lacrimal and salivary glands. Dry eyes and dry mouths are the most common symptoms due to the loss of salivary and lacrimal gland function. Symptoms become more severe in secondary SS, where SS is present along with other autoimmune diseases like systemic lupus erythematosus, systemic sclerosis, or rheumatoid arthritis. It is known that aberrant activation of immune cells plays an important role in disease progression, however, the mechanism for these pathological changes in the immune system remains largely unknown. This review highlights the role of different immune cells in disease development, therapeutic treatments, and future strategies that are available to target various immune cells to cure the disease.
Physiological and Differential Proteomic Analyses of Imitation Drought Stress Response in Sorghum bicolor Root at the Seedling Stage
Authors: Li Hongbing , Li Yulin , Ke Qingbo , Kwak Sang-Soo , Zhang Suiqi , Deng Xiping
Published: December 2020

Drought is one of the most important constraints on the growth and productivity of many crops, including sorghum. However, as a primary sensing organ, the plant root response to drought has not been well documented at the proteomic level. In the present study, we compared physiological alteration and differential accumulation of proteins in the roots of sorghum (Sorghum bicolor) inbred line BT×623 response to Polyethylene Glycol (PEG)-induced drought stress at the seedling stage. Drought stress (up to 24 h after PEG treatment) resulted in increased accumulation of reactive oxygen species (ROS) and subsequent lipid peroxidation. The proline content was increased in drought-stressed plants. The physiological mechanism of sorghum root response to drought was attributed to the elimination of harmful free radicals and to the alleviation of oxidative stress via the synergistic action of antioxidant enzymes, such as superoxide dismutase, peroxidase, and polyphenol oxidase. The high-resolution proteome map demonstrated significant variations in about 65 protein spots detected on Coomassie Brilliant Blue-stained 2-DE gels. Of these, 52 protein spots were identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-TOF MS) representing 49 unique proteins; the levels of 43 protein spots were increased, and 22 were decreased under drought condition. The proteins identified in this study are involved in a variety of cellular functions, including carbohydrate and energy metabolism, antioxidant and defense response, protein synthesis/processing/degradation, transcriptional regulation, amino acid biosynthesis, and nitrogen metabolism, which contribute jointly to the molecular mechanism of outstanding drought tolerance in sorghum plants. Analysis of protein expression patterns and physiological analysis revealed that proteins associated with changes in energy usage; osmotic adjustment; ROS scavenging; and protein synthesis, processing, and proteolysis play important roles in maintaining root growth under drought stress. This study provides new insight for better understanding of the molecular basis of drought stress responses, aiming to improve plant drought tolerance for enhanced yield.
RB Regulates DNA Double Strand Break Repair Pathway Choice by Mediating CtIP Dependent End Resection
Authors: Jiang Yuning , Yam Jason C. , Tham Clement C. , Pang Chi Pui , Chu Wai Kit
Published: December 2020

Inactivation of the retinoblastoma tumor suppressor gene (RB1) leads to genome instability, and can be detected in retinoblastoma and other cancers. One damaging effect is causing DNA double strand breaks (DSB), which, however, can be repaired by homologous recombination (HR), classical non-homologous end joining (C-NHEJ), and micro-homology mediated end joining (MMEJ). We aimed to study the mechanistic roles of RB in regulating multiple DSB repair pathways. Here we show that HR and C-NHEJ are decreased, but MMEJ is elevated in RB-depleted cells. After inducing DSB by camptothecin, RB co-localizes with CtIP, which regulates DSB end resection. RB depletion leads to less RPA and native BrdU foci, which implies less end resection. In RB-depleted cells, less CtIP foci, and a lack of phosphorylation on CtIP Thr847, are observed. According to the synthetic lethality principle, based on the altered DSB repair pathway choice, after inducing DSBs by camptothecin, RB depleted cells are more sensitive to co-treatment with camptothecin and MMEJ blocker poly-ADP ribose polymerase 1 (PARP1) inhibitor. We propose a model whereby RB can regulate DSB repair pathway choice by mediating the CtIP dependent DNA end resection. The use of PARP1 inhibitor could potentially improve treatment outcomes for RB-deficient cancers.
Serenoa repens and Urtica dioica Fixed Combination: In-Vitro Validation of a Therapy for Benign Prostatic Hyperplasia (BPH)
Authors: Saponaro Miriam , Giacomini Isabella , Morandin Giulia , Cocetta Veronica , Ragazzi Eugenio , Orso Genny , Carnevali Ilaria , Berretta Massimiliano , Mancini Mariangela , Pagano Francesco , Montopoli Monica
Published: December 2020

Benign prostatic hyperplasia (BPH) is an age-related chronic disorder, characterized by the hyperproliferation of prostatic epithelial and stromal cells, which drives prostate enlargement. Since BPH aetiology and progression have been associated with the persistence of an inflammatory stimulus, induced both by Nuclear Factor-kappa B (NF-κB) activation and reactive oxygen species (ROS) production, the inhibition of these pathways could result in a good tool for its clinical treatment. This study aimed to evaluate the antioxidant and anti-inflammatory activity of a combined formulation of Serenoa repens and Urtica dioica (SR/UD) in an in vitro human model of BPH. The results confirmed both the antioxidant and the anti-inflammatory effects of SR/UD. In fact, SR/UD simultaneously reduced ROS production, NF-κB translocation inside the nucleus, and, consequently, interleukin 6 (IL-6) and interleukin 8 (IL-8) production. Furthermore, the effect of SR/UD was also tested in a human androgen-independent prostate cell model, PC3. SR/UD did not show any significant antioxidant and anti-inflammatory effect, but was able to reduce NF-κB translocation. Taken together, these results suggested a promising role of SR/UD in BPH and BPH-linked disorder prevention.
Phospho-Mutant Activity Assays Provide Evidence for the Negative Regulation of Transcriptional Regulator PRE1 by Phosphorylation
Authors: Wang Minmin , Tian Yanchen , Han Chao , Zhou Chuanen , Bai Ming-Yi , Fan Min
Published: December 2020

The PACLOBUTRAZOL-RESISTANCE (PRE) gene family encodes a group of atypical helix-loop-helix (HLH) proteins that act as the major hub integrating a wide range of environmental and hormonal signals to regulate plant growth and development. PRE1, as a positive regulator of cell elongation, activates HBI1 DNA binding by sequestering its inhibitor IBH1. Furthermore, PRE1 can be phosphorylated at Ser-46 and Ser-67, but how this phosphorylation regulates the functions of PRE1 remains unclear. Here, we used a phospho-mutant activity assay to reveal that the phosphorylation at Ser-67 negatively regulates the functions of PRE1 on cell elongation. Both of mutations of serine 46, either to phospho-dead alanine or phospho-mimicking glutamic acid, had no significant effects on the functions of PRE1. However, the mutation of serine 67 to glutamic acid (PRE1S67E-Ox), but not alanine (PRE1S67A-Ox), significantly reduced the promoting effects of PRE1 on cell elongation. The mutation of Ser-67 to Glu-67 impaired the interaction of PRE1 with IBH1 and resulted in PRE1 failing to inhibit the interaction between IBH1 and HBI1, losing the ability to induce the expression of the subsequent cell elongation-related genes. Furthermore, we showed that PRE1-Ox and PRE1S67A-Ox both suppressed but PRE1S67E-Ox had no strong effects on the dwarf phenotypes of IBH1-Ox. Our study demonstrated that the PRE1 activity is negatively regulated by the phosphorylation at Ser-67.
HSP70-Homolog DnaK of Pseudomonas aeruginosa Increases the Production of IL-27 through Expression of EBI3 via TLR4-Dependent NF-κB and TLR4-Independent Akt Signaling
Authors: Jeon Jisu , Lee Yeji , Yu Hyeonseung , Ha Un-Hwan
Published: December 2020

IL-27, a heterodimeric cytokine composed of the p28 subunit and Epstein–Barr virus-induced gene 3 (EBI3), acts as a potent immunosuppressant and thus limits pathogenic inflammatory responses. IL-27 is upregulated upon Pseudomonas aeruginosa infection in septic mice, increasing susceptibility to the infection and decreasing clearance of the pathogen. However, it remains unclear which P. aeruginosa-derived molecules promote production of IL-27. In this study, we explored the mechanism by which P. aeruginosa DnaK, a heat shock protein 70-like protein, induces EBI3 expression, thereby promoting production of IL-27. Upregulation of EBI3 expression did not lead to an increase in IL-35, which consists of the p35 subunit and EBI3. The IL-27 production in response to DnaK was biologically active, as reflected by stimulation of IL-10 production. DnaK-mediated expression of EBI3 was driven by two distinct signaling pathways, NF-κB and Akt. However, NF-κB is linked to TLR4-associated signaling pathways, whereas Akt is not. Taken together, our results reveal that P. aeruginosa DnaK potently upregulates EBI3 expression, which in turn drives production of the prominent anti-inflammatory cytokine IL-27, as a consequence of TLR4-dependent activation of NF-κB and TLR4-independent activation of the Akt signaling pathway.