bluuebunny/crossref_metadata_2025 huggingface dataset:
Tumor Microenvironmental Cytokines Drive NSCLC Cell Aggressiveness and Drug-Resistance via YAP-Mediated Autophagy
Authors: Matarrese Paola , Vona Rosa , Ascione Barbara , Cittadini Camilla , Tocci Annalisa , Mileo Anna Maria
Published: March 2023

Dynamic reciprocity between cellular components of the tumor microenvironment and tumor cells occurs primarily through the interaction of soluble signals, i.e., cytokines produced by stromal cells to support cancer initiation and progression by regulating cell survival, differentiation and immune cell functionality, as well as cell migration and death. In the present study, we focused on the analysis of the functional response of non-small cell lung cancer cell lines elicited by the treatment with some crucial stromal factors which, at least in part, mimic the stimulus exerted in vivo on tumor cells by microenvironmental components. Our molecular and functional results highlight the role played by the autophagic machinery in the cellular response in terms of the invasive capacity, stemness and drug resistance of two non-small lung cancer cell lines treated with stromal cytokines, also highlighting the emerging role of the YAP pathway in the mutual and dynamic crosstalk between tumor cells and tumor microenvironment elements. The results of this study provide new insights into the YAP-mediated autophagic mechanism elicited by microenvironmental cytokines on non-small cell lung cancer cell lines and may suggest new potential strategies for future cancer therapeutic interventions.
Current Strategy to Treat Immunogenic Gastrointestinal Cancers: Perspectives for a New Era
Authors: Shimozaki Keitaro , Nakayama Izuma , Hirota Toru , Yamaguchi Kensei
Published: March 2023

Since pembrolizumab, an anti-programmed death-1 (PD-1) antibody, showed a dramatic response to immunogenic cancers with microsatellite instability-high (MSI-H) and/or deficient mismatch repair (dMMR) in the pilot clinical trial KEYNOTE-016, subsequent studies have confirmed durable responses of anti-PD-1 inhibitors for MSI-H/dMMR solid tumors. As immunotherapy is described as a “game changer,” the therapeutic landscape for MSI-H/dMMR solid tumors including gastrointestinal cancers has changed considerably in the last decade. An MSI/MMR status has been established as the predictive biomarker for immune checkpoint blockades, playing an indispensable role in the clinical practice of patients with MSI-H/dMMR tumors. Immunotherapy is also now investigated for locally advanced MSI-H/dMMR gastrointestinal cancers. Despite this great success, a few populations with MSI-H/dMMR gastrointestinal cancers do not respond to immunotherapy, possibly due to the existence of intrinsic or acquired resistance mechanisms. Clarifying the underlying mechanisms of resistance remains a future task, whereas attempts to overcome resistance and improve the efficacy of immunotherapy are currently ongoing. Herein, we review recent clinical trials with special attention to MSI-H/dMMR gastrointestinal cancers together with basic/translational findings, which provide their rationale, and discuss perspectives for the further therapeutic development of treatment in this field.
The Influence of the Burning Environment on the Properties of Ceramic Products Based on Fusible Raw Materials
Authors: Zhuginissov Maratbek T. , Nurlybayev Ruslan E. , Orynbekov Yelzhan S. , Zhumadilova Zhanar O. , Khamza Yerlan Y. , Bulenbayev Maxat Z.
Published: March 2023

The purpose of this work was to investigate the effect of the burning environment on the properties and phase composition of clinker-based ceramic specimens made from loam with diatomite and bentonite clay in order to develop technological parameters for the manufacture of clinker products. The main raw material used for the experiments was local fusible loam from the Almaty deposit, which is the basis for the production of 75 and 100 grade ceramic bricks. Diatomite from Utesai deposit (Aktobe region) and highly plastic bentonite clay from Darbazin deposit (Turkestan region) were used as additives. Loam and bentonite clay were applied after grinding and sieving through a 1 mm sieve. Diatomite was applied after grinding and milling until it had completely passed through a 0.315 mm sieve. The raw materials are mixed after dosing, and then water is added in the amount required until a pliable mass is obtained. To investigate the properties of the products, standard cylinder samples were prepared with a diameter of 50 mm and a height of 50 mm. The cylinder samples were pressed on a hydraulic press at a pressure of 2–4 kN. The samples were dried in a desiccator at 95–100 °C for 2 h. After drying, the products were burning in a muffle kiln. The analysis of the properties of the burned products showed that the optimum ratio in the ceramic mixture of loam and diatomite to loam and bentonite clay is 85%:15%. After burning in a slightly oxidising environment at 1170 °C, the ceramic specimens correspond to Class 2 for the medium-density and compressive strength grades M 400 and M 500 (GOST 530-2012 (Government standard). X-ray diffractometric analysis showed that the products contain augite, quartz and anorthite as crystalline phases, with the former predominating. After burning in a reducing atmosphere, at 1170 °C, the properties of the samples have higher values compared to the samples burning in a weakly oxidising atmosphere. The resulting properties, according to the regulations, are characteristic of clinker bricks (DIN V 105-1 (technical requirements for clinker façade bricks). After burning in a reducing environment, the phase composition of the products changes qualitatively; in addition to augite and quartz, albite, diopside, orthoclase and haematite are present in the samples.
Preparation and Spectroscopic Characterization of Ternary Inclusion Complexes of Ascorbyl Palmitate and Urea with γ-Cyclodextrin
Authors: Inoue Yutaka , Nanri Ayumi , Arce Florencio Jr. , See Gerard Lee , Tanikawa Takashi , Yokogawa Takami , Kitamura Masashi
Published: March 2023

A three-component inclusion complex of ascorbyl palmitate (ASCP), urea (UR), and γ-cyclodextrin (γCD) with a molar ratio of 1/12 has been prepared for the first time using the evaporation method (EVP method) and the grinding and mixing method (GM method). Also, we investigated changes in the physicochemical properties of the three-component complexes. The powder X-ray diffraction (PXRD) measurements showed ASCP, UR, and γCD characteristic peaks in the physical mixture (PM) (AU (ASCP/UR = 1/12)/γCD = 1/2). In GM (AU (ASCP/UR = 1/12)/γCD = 1/1), new diffraction peaks were observed around 2θ = 7.5° and 16.6°, while characteristic peaks derived from EVP (ASCP/UR = 1/12) were observed at 2θ = 23.4° and 24.9°. On the other hand, new diffraction peaks at 2θ = 7.4° and 16.6° were observed in GM (1/2). In the differential scanning calorimeter (DSC) measurement, an endothermic peak at around 83 °C was observed in the GM (1/1) sample, which is thought to originate from the phase transition of urea from the hexagonal to the tetragonal form. An endothermic peak around 113.9 °C was also observed for EVP (ASCP/UR = 1/12). However, no characteristic phase transition-derived peak or EVP (ASCP/UR = 1/12)-derived endothermic peak was observed in GM (1/2). Near infrared (NIR) spectroscopy of GM (1/2) showed no shift in the peak derived from the CH group of ASCP. The peaks derived from the NH group of UR shifted to the high and low wavenumber sides at 5032 cm−1 and 5108 cm−1 in EVP (ASCP/UR = 1/12). The peak derived from the OH group of γCD shifted, and the peak derived from the OH group of ASCP broadened at GM (1/2). These results suggest that AU (ASCP/UR = 1/12)/γCD prepared by the mixed grinding method formed inclusion complexes at the molar ratio (1/2).
Combining Impedance Spectroscopy and Information Visualization Methods to Optimize the Detection of Carbendazim Using Layer-by-Layer Films
Authors: Furini Leonardo Negri , Fernandes José Diego , Vieira Douglas Henrique , Morato Luis Fernando do Carmo , Alves Neri , Constantino Carlos José Leopoldo
Published: March 2023

Usually, electronic tongues (e-tongue) do not require specific interactions to discriminate aqueous solutions. Among the several factors which determine the electrical properties of sensing units, the interactions between liquids and interfaces have a crucial role. Here, we explore the interaction between dioctadecyldimethylammonium bromide (DODAB) lipid and carbendazim (MBC) pesticide in an e-tongue to discriminate different MBC concentrations in aqueous solutions. The sensing units were fabricated of gold interdigitated electrodes (IDEs) coated with layer-by-layer (LbL) films of DODAB and nickel tetrasulfonated phthalocyanine (NiTsPc), perylene and 1,2-dipalmitoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DPPG), namely (DODAB/NiTsPc)5 and (Perylene/DPPG)5, respectively. Besides, a bare electrode also constituted the e-tongue to distinguish MBC concentrations from 1.0 × 10−7 up to 1.0 × 10−10 mol L−1, by impedance spectroscopy. In addition, the experiment was optimized using two IDE geometries. The LbL films were manually fabricated obtaining linear growth monitored via UV-Vis absorption spectroscopy. Optical images associated with chemical mapping reveals the presence of small aggregates in the DODAB/NiTsPc LbL film surface. Although the e-tongue was able to discriminate all MBC concentrations by means of the interactive document map (IDMAP), only the sensing unit covered with DODAB/NiTsPc LbL film presented a satisfactory response. According to the equivalent circuit, the main contribution arises from the bulk and film surface due to the interaction between DODAB and MBC, indicating THE sensitivity of the sensing unit. Finally, the adsorption of MBC molecules onto the film surface induced an irreversible process, although there are some frequencies at which the sensing unit response seems to be reversible, as shown by parallel coordinates.
Development and Pilot Study of a Pediatric Screening for Feeding and Swallowing Disorders in Infants and Children: The Pediatric Screening–Priority Evaluation Dysphagia (PS–PED)
Authors: Cerchiari Antonella , Tofani Marco , Giordani Carolina , Franceschetti Silvia , Capuano Eleonora , Pizza Francesca , Della Bella Gessica , Raponi Massimiliano , Biondo Giorgia
Published: March 2023

Feeding and swallowing disorders (FSD) are common during childhood, with a prevalence of 85% in children with neurodevelopmental disorders. A comprehensive screening is essential to identify FSD and improve health outcomes in a clinical setting. This study aims to develop a new Pediatric Screening tool capable of identifying FSD. This screening tool was developed in three steps: selecting variables based on clinical experience, searching the literature and finding agreement between experts with a two-round Delphi study. This process, which reached 97% of agreement between experts, led to the development of the Pediatric Screening–Priority Evaluation Dysphagia (PS–PED). PS–PED comprises 14 items divided into three main domains: clinical history, health status and feeding condition. We also carried out a pilot test for measuring internal consistency, as measured with Cronbach Coefficient alpha. Concurrent validity, as measured with Pearson correlation coefficient, was tested using a videofluoroscopy swallow study (VFSS) classified with the Penetration Aspiration Scale (PAS). The pilot test was conducted on 59 children with different health conditions. Our findings showed good internal consistency (alpha = 0.731), and a strong linear correlation with PAS (Pearson 0.824). Furthermore, comparing PS–PED and PAS scores, we find preliminary strong discriminant validity to identify children with FSD (p < 0.01). Our results provide evidence on using the 14-item PS–PED as a screening tool for FSD in a clinical sample of children with heterogeneous disease.
Cumulative Radiation Dose from Medical Imaging in Children with Congenital Heart Disease: A Systematic Review
Authors: Shelly Emer , Waldron Michael G. , Field Erica , Moore Niamh , Young Rena , Scally Andy , England Andrew , Maher Michael , McEntee Mark F.
Published: March 2023

Children with congenital heart disease are exposed to repeated medical imaging throughout their lifetime. Although the imaging contributes to their care and treatment, exposure to ionising radiation is known to increase one’s lifetime attributable risk of malignancy. A systematic search of multiple databases was performed. Inclusion and exclusion criteria were applied to all relevant papers and seven were deemed acceptable for quality assessment and risk of bias assessment. The cumulative effective dose (CED) varied widely across the patient cohorts, ranging from 0.96 mSv to 53.5 mSv. However, it was evident across many of the included studies that a significant number of patients were exposed to a CED >20 mSv, the current annual occupational exposure limit. Many factors affected the dose which patients received, including age and clinical demographics. The imaging modality which contributed the most radiation dose to patients was cardiology interventional procedures. Paediatric patients with congenital heart disease are at an increased risk of receiving an elevated cumulative radiation dose across their lifetime. Further research should focus on identifying risk factors for receiving higher radiation doses, keeping track of doses, and dose optimisation where possible.
PANDAS/PANS in the COVID-19 Age: Autoimmunity and Epstein–Barr Virus Reactivation as Trigger Agents?
Authors: Pallanti Stefano , Di Ponzio Michele
Published: March 2023

COVID-19 impacted the entire world’s population, frequently resulting in long-lasting neuropsychiatric complications. Furthermore, social distancing, lockdowns and fear for one’s personal health worsen individual psychological wellbeing, especially in children and adolescents. Herein, we discuss the results of studies that specifically reported data about the impact of the COVID-19 pandemic or infection on children with Pediatric Acute-Onset Neuropsychiatric Disorders (PANS). Furthermore, we present the cases of five adolescents with PANS whose symptomatology increased following SARS-CoV-2 infection. What emerged from this study was that COVID-19 resulted in the exacerbation of obsessions, tics, anxiety and mood symptoms and decreased wellbeing. Moreover, new symptoms, as well as new PANS cases, are reported to have arisen after COVID-19 infection. Here, we hypothesize that the pathogenic mechanisms of silent viruses, such as the Epstein–Barr virus, are related to neuroinflammation, immune responses and reactivation, with additional roles played by social-isolation-related inflammatory processes. The discussion of PANS, which represents a model of immune-mediated neuropsychiatric manifestations, is particularly relevant, with the aim of uncovering the mechanisms that lead to neuropsychiatric Post-Acute COVID-19 Syndrome (PACS). Prospects for future studies and treatment implications are discussed.
Retrocardiac Pneumomediastinum: Description of an Unusual Case and Review of Literature
Authors: Porcaro Federica , Onofri Alessandro , Allegorico Annalisa , Tomà Paolo , Cutrera Renato
Published: March 2023

Retrocardiac pneumomediastinum (RP) is the consequence of air trapping in the inferior and posterior mediastinum. It is characterized by the presence of a right or left para-sagittal infrahilar oval or pyramidal air collection on a chest X-ray. It is usually detected in neonates because of alveolar rupture after invasive ventilation or invasive manoeuvres applied on airways or the digestive tract. A healthy child came to the emergency department (ED) for acute respiratory failure due to viral bronchiolitis when he was 2 months old. Because of his clinical condition, he underwent helmet continuous positive airway pressure (HCPAP). When the condition allowed, he was discharged and sent home. He was re-admitted into the hospital for asthmatic bronchitis 3 months later. A frontal chest X-ray taken during the second hospitalization showed an oval-shaped retrocardiac air lucency not previously detected. Differential diagnosis including digestive and lung malformations was made. Finally, the diagnosis of RP was made. We report an unusual case of retrocardiac pneumomediastinum in a 5-month-old male infant after the application of continuous positive pressure via a helmet. RP presentation after the administration of non-invasive ventilatory support beyond the neonatal age is unusual. Although surgical drainage is curative, conservative treatment can be considered in hemodynamically stable patients.
Physical Fitness and Body Composition of Youth Referees in Invasion Games
Authors: Gouveia Élvio Rúbio , Lopes Helder , Nascimento Marcelo de Maio , Clemente Filipe Manuel , Sarmento Hugo , Ihle Andreas , Ferrari Gerson , Luz Pedro , Caldeira Henrique , Marques Adilson
Published: March 2023

Background: Referees’ activity can be an instrument for training students with an impact on health-related physical fitness (PF). This study aimed to investigate the differences in PF and body composition between students without sports practice (G1), students with regular sports practice (G2), and student referees in team invasion games (G3). Methods: This study followed a cross-sectional design. The sample comprised 45 male students between 14 and 20 years old (16.40 ± 1.85). Three groups (G1, G2, and G3) of 15 participants each were selected. PF was assessed by a 20 m shuttle run, change-of-direction test, and standing long jump. Body composition was determined by body mass index (BMI; kg/m2) and the percentage of body fat (%BF) predicted by skinfold. Results: After controlling for age as a covariate, the block of variables used to characterize PF was statistically different between sports practice groups favoring student referees (p < 0.001; r = 0.26). Similar results were achieved for body composition (i.e., BMI and %BF) (p < 0.001; r = 0.17). However, when the dependent variables were evaluated separately, there were only differences between groups in %BF (p = 0.007; r = 0.21). Student referees had statistically significantly lower values than the remaining groups. Conclusion: Refereeing activity benefits PF related to health and performance, including body composition. This study confirms the benefits related to health in children and adolescents who are involved in refereeing activity.
Delayed and Interrupted Ventilation with Excess Suctioning after Helping Babies Breathe with Congolese Birth Attendants
Authors: Patterson Jackie K. , Ishoso Daniel , Eilevstjønn Joar , Bauserman Melissa , Haug Ingunn , Iyer Pooja , Kamath-Rayne Beena D. , Lokangaka Adrien , Lowman Casey , Mafuta Eric , Myklebust Helge , Nolen Tracy , Patterson Janna , Tshefu Antoinette , Bose Carl , Berkelhamer Sara
Published: March 2023

There is a substantial gap in our understanding of resuscitation practices following Helping Babies Breathe (HBB) training. We sought to address this gap through an analysis of observed resuscitations following HBB 2nd edition training in the Democratic Republic of the Congo. This is a secondary analysis of a clinical trial evaluating the effect of resuscitation training and electronic heart rate monitoring on stillbirths. We included in-born, liveborn neonates ≥28 weeks gestation whose resuscitation care was directly observed and documented. For the 2592 births observed, providers dried/stimulated before suctioning in 97% of cases and suctioned before ventilating in 100%. Only 19.7% of newborns not breathing well by 60 s (s) after birth ever received ventilation. Providers initiated ventilation at a median 347 s (>five minutes) after birth; no cases were initiated within the Golden Minute. During 81 resuscitations involving ventilation, stimulation and suction both delayed and interrupted ventilation with a median 132 s spent drying/stimulating and 98 s suctioning. This study demonstrates that HBB-trained providers followed the correct order of resuscitation steps. Providers frequently failed to initiate ventilation. When ventilation was initiated, it was delayed and interrupted by stimulation and suctioning. Innovative strategies targeting early and continuous ventilation are needed to maximize the impact of HBB.
Effect of Solid Sodium Silicate on Workability, Hydration and Strength of Alkali-Activated GGBS/Fly Ash Paste
Authors: Dong Tingkai , Sun Tao , Xu Fang , Ouyang Gaoshang , Wang Hongjian , Yang Fan , Wang Ziyan
Published: March 2023

Based on economic and environmental considerations, the recycling economy of mineral waste has been found to have great potential and economic benefits worldwide, in which alkali-activated cementitious materials are one of the main developing directions. The alkali activators commonly used in alkali-activated cementitious materials are the composite activators of sodium silicate solution and solid sodium hydroxide, which not only need to deal with high viscosity and corrosive chemicals, but also need to be prepared in advance and properly stored. In this paper, ground granulated blast furnace slag (GGBS) and fly ash were used as precursors, while solid sodium silicate powder was applied as the alkali activator. In addition, the precursors were mixed with the activator in advance and activated by adding water to prepare alkali-activated GGBS/fly ash cement. The influence of precursor components, the dosage of the alkali activator and the liquid–solid ratio on the working performance, mechanical strength and hydration process of alkali-activated cement was studied. The results showed that the further incorporation of GGBS accelerated the alkali activation reaction rate and improved the strength of the specimen. However, in the specimen with GGBS as the main component of the precursor, the main hydration product was C-A-S-H gel, which was different in the structural order and quantity. The compressive strength indicated that there was the best amount of activator to match it in terms of the precursor with certain components. A too high or too low amount of activator will hinder the alkali activation reaction. This study can provide some significant reference material for the use of solid alkali activators in alkali-activated cementitious materials.
Development and Studying of the Technology for Thermal Spraying of Coatings Made from Ultra-High-Molecular-Weight Polyethylene
Authors: Skakov Mazhyn , Ocheredko Igor , Tuyakbayev Baurzhan , Bayandinova Moldir , Nurizinova Makpal
Published: March 2023

UHMWPE is resistant to acids, alkalis and radiation. Its combination of unique properties makes this material attractive for obtaining multifunctional coatings. However, in practice, obtaining coatings based on UHMWPE is associated with difficulties associated with low thermal conductivity and high viscosity of the material. The possibility of overcoming the technological problems of obtaining gas-thermal coatings based on UHMWPE was studied in the present work. A physical model of a flame with UHMWPE particles moving along the central axis was developed by the finite element method. The temperature along the central axis of the plume was determined. The interaction between a gas-thermal torch and a UHMWPE particle was established. It was determined that the residence time of UHMWPE particles in a gas-thermal flame is not enough for its complete penetration, which is the reason for the appearance of various defects. The interrelation of the particle heating rate in the torch depending on its diameter was determined. A new variant of coating deposition with preliminary heating of the powder in a fluidized bed was proposed. The thermal characteristics of UHMWPE powder were determined by differential scanning calorimetry and thermogravimetric analysis. The allowable temperature interval for UHMWPE deposition was established. Coatings were obtained under various deposition modes. It was established using the methods of X-ray diffraction analysis and infrared spectroscopy that the structure of the crystal lattice of UHMWPE did not change after deposition. Significant oxidation processes do not occur during spraying. It was found using scanning electron microscopy that the coatings obtained with preliminary heating of the powder in a fluidized bed do not have air inclusions. The obtained results make it possible to obtain higher quality coatings.
Tuning the Wettability of a Commercial Silane Product to Induce Superamphiphobicity for Stone Protection
Authors: Manoudis Panagiotis N. , Chughtai Zebunnisa , Tsiridis Vasilios , Evgenidis Sotiris P. , Spathis Panagiotis K. , Karapantsios Thodoris D. , Karapanagiotis Ioannis
Published: March 2023

Silane-based materials are used for the protection of heritage and modern buildings. A versatile method is developed to tune the wetting properties of a typical silane-based material from hydrophobicity to superamphiphobicity, thus enhancing the protective efficacy against rainwater and organic pollutants. A commercially available silane product is blended with a fluoropolymer to lower the surface energy and silica (SiO2) nanoparticles to affect the surface morphologies of the produced coatings on marble. Contact angles of water and oil drops are measured on the coating surfaces which were prepared using 16 different combinations of fluoropolymer and nanoparticle concentrations. It is shown that the synergistic effect of surface structure and chemistry can lead to the production of coatings that possess superamphiphobic properties. The wetting properties of a selected non-wettable coating are further characterised using a custom-made, fully-automated device (Kerberos) which monitors simultaneously the deformation of the liquid interface, spreading and sliding of the drop along the sample surface during tilting. Several tests are carried out to evaluate the durability of the selected superamphiphobic coating, offering overall promising results. The versatile method can be used to impart superamphiphobicity to the surfaces of various materials. The method developed herein can be adopted to tune the wetting properties of other silane-based commercial products which are used for the protection of buildings.
Optimization of a Fuzzy System Used to Characterize the Factors That Affect Drivers on Urban Roads
Authors: Bejarano Lilian Astrid , Montenegro Carlos Enrique , Espitia Helbert Eduardo
Published: March 2023

This document seeks to model the behavior of drivers on urban roads considering different environmental factors using a Mamdani-type fuzzy system. For this, a leader-following traffic model and a fuzzy logic system are used to characterize the behavior of drivers. Real data are obtained using a camera in the roads under consideration, and these data and an optimization process are employed to fit the fuzzy model. For the optimization process, the fuzzy logic system used to model the driver’s behavior is incorporated into a dynamic vehicle tracking model where the fuzzy system allows considering different environmental factors in the traffic model simulation. After carrying out the optimization process, it is possible to assign linguistic labels to the fuzzy sets associated with the output. In this way, the interpretability of the proposed fuzzy system is achieved by assigning labels (concepts) to the fuzzy sets. The results show that the proposed model fits the real data, and the fuzzy sets are adjusted according to the measured data for the different considered cases.
Comparative Analysis and Classification of SARS-CoV-2 Spike Protein Structures in PDB
Authors: Aslam Memoona , Nawaz M. Saqib , Fournier-Viger Philippe , Li Wenjin
Published: March 2023

The Spike (S) protein of the SARS-CoV-2 virus that causes the COVID-19 disease is considered the most important target for vaccine, drug and therapeutic research as it attaches and binds to the ACE2 receptor of the host cells and allows the entry of this virus. Analysis and classification of newly determined S protein structures for SARS-CoV-2 are critical to properly understand their functional, evolutionary and architectural relatedness to already known protein structures. In this paper, first, the comparative analysis of SARS-CoV-2 S protein structures is performed. Through comparative analysis, the S protein structures in the PDB (protein data bank) database are compared and analyzed not only with each other but with the structures of other viruses for various parameters. Second, the S protein structures in PDB are classified into different variants, and the associated published literature is studied to investigate what kind of therapeutics (antibodies, T-cell receptors and small molecules) are used on the structures. This is the first study that classifies the S protein structures of the SARS-CoV-2 in PDB into various variants, and the obtained comparative analysis results could be beneficial to the research community, in general, and to crystallographers and health workers, in particular.
Effects of Crop Rotation and Tillage on Winter Wheat Growth and Yield under Cold Dryland Conditions
Authors: Lotfi Ramin , Pessarakli Mohammad
Published: March 2023

To investigate responses of two winter wheat genotypes under different crop management systems (rotation and tillage), a split–split plot experiment was conducted based on a randomized complete block design (RCBD) with three replications during 4 years in Maragheh, Iran. Three crop rotation treatments [vetch–wheat (V–W), chickpea–wheat (C–W), and safflower–wheat (S–W)] were considered in main plots, three tillage treatments (conventional-tillage (CT), minimum-tillage (MT), and no-tillage (NT)) were located in subplots, and two winter dryland wheat genotypes (Baran and Azar2) were allocated in sub-sub plots. Results indicated that soil moisture content in NT was greater than that in MT and CT. The highest relative water content (RWC), normalized difference vegetative index (NDVI), stomatal conductance (gs), and transpiration rate (E) were obtained from the Baran genotype in the V–W rotation under NT. In the last year of the experiment, rainfall productivity in NT treatment improved by 32%, compared to CT. The Baran genotype had higher rainfall productivity in both MT and NT treatments with 0.71 and 0.70 kg m−3, respectively. Crop water requirement was not affected by crop rotation or tillage treatments. Maximum grain yields in V–W, C–W, and S–W rotations were recorded as 2231, 2105, and 1991 kg ha−1, respectively. With increasing soil moisture storage and improving rainfall productivity under full implementation of conservation agriculture components (after 4 years), grain yield of Baran and Azar2 improved in NT compared to that of CT by about 6–9% and 6–14%, respectively. Therefore, the application of V–W rotation with NT in cold dryland areas is recommended for developing of conservation agriculture system.
The Effect of Process Parameters on the Properties and Microstructure of A380 Aluminum Alloy Casting with Different Wall Thicknesses
Authors: Li He , Zhang Han , Peng Wenfei , Lin Bo , Shao Yiyu , Lin Longfei , Fu Bangjie , Yu Ziming
Published: March 2023

In the present work, the effects of different die-casting process parameters on the mechanical properties and microstructure of A380 aluminum alloy casting with different wall thicknesses during the solidification process have been experimentally investigated. The experimental results show that both boost pressure and injection speed have a significant effect on the mechanical properties of the casting. As the injection speed increases, the changes in mechanical properties are more significant in the thin-walled area, while increasing the boosting pressure has a greater effect on the mechanical properties of the thick-walled area. In addition, the evolution of microstructure composition, including the α-Al phase, eutectic Si phase and Al-Si-Fe-Mn phase, has been analyzed and compared by energy-dispersive spectroscopy (EDS), optical microscopy (OM) and scanning electron microscopy (SEM). It was found that the α-Al phase in the thin-walled area is significantly refined with the increase of injection speed. Meanwhile, with the increase of boost pressure, the α-Al phase in the thick-walled area gradually becomes finer, and the distribution of the eutectic Si phase and the Al-Si-Fe-Mn phase in the alloy becomes more uniform. Thus, the injection speed and boost pressure have an important impact on the overall forming quality of the casting.
Use of Microbially Treated Olive Mill Wastewaters as Soil Organic Amendments; Their Short-Term Effects on the Soil Nematode Community
Authors: Dimou Maria D. , Monokrousos Nikolaos , Katapodis Petros , Diamantopoulou Panagiota A. , Argyropoulou Maria D. , Papatheodorou Efimia M.
Published: March 2023

Managing olive mill wastewater (OMW) is a major environmental problem. We followed two methods for OMW bioremediation: one with the white-rot fungus Pleurotus ostreatus alone and one with the fungus plus the microalga Chlorella vulgaris. To evaluate the potential use of both final products as soil amendments, in a pot experiment, we applied treated OMW to soil cultivated with Lactuca sativa, and we studied their short-term effects on the soil nematode community in terms of trophic and functional structure, metabolic footprint, genera composition, and interaction networks. We also applied non-treated OMW and simply water (control). The addition of non-treated OMW significantly reduced the abundance of all nematodes, and the network of interactions was the most fragmented and the least robust against future disturbance. The effect on trophic group abundances was similar but less pronounced when OMW was previously detoxified either by the fungus alone or by its combination with the alga. In the latter case, the phytoparasites were suppressed but the bacterivorous nematodes were not affected. However, the most cohesive and robust nematode network was formed in the soil that received the fungal-treated OMW. None of our OMW applications significantly changed community composition, none improved the already degraded status of the soil food web—which is attributed to the sandy texture of our soil—and none affected the growth of lettuce plants, perhaps because of the short duration of the experiment (30 days). Thus, our future research will aim to estimate the long-term impact of OMW.
Pre-Analytical Evaluation of Streck Cell-Free DNA Blood Collection Tubes for Liquid Profiling in Oncology
Authors: Diaz Inga Medina , Nocon Annette , Held Stefanie A. E. , Kobilay Makbule , Skowasch Dirk , Bronkhorst Abel J. , Ungerer Vida , Fredebohm Johannes , Diehl Frank , Holdenrieder Stefan , Holtrup Frank
Published: March 2023

Excellent pre-analytical stability is an essential precondition for reliable molecular profiling of circulating tumor DNA (ctDNA) in oncological diagnostics. Therefore, in vitro degradation of ctDNA and the additional release of contaminating genomic DNA from lysed blood cells must be prevented. Streck Cell-Free DNA blood collection tubes (cfDNA BCTs) have proposed advantages over standard K2EDTA tubes, but mainly have been tested in healthy individuals. Blood was collected from cancer patients (n = 53) suffering from colorectal (n = 21), pancreatic (n = 11), and non-small-cell lung cancer (n = 21) using cfDNA BCT tubes and K2EDTA tubes that were processed immediately or after 3 days (BCTs) or 6 hours (K2EDTA) at room temperature. The cfDNA isolated from these samples was characterized in terms of yield using LINE-1 qPCR; the level of gDNA contamination; and the mutation status of KRAS, NRAS, and EGFR genes using BEAMing ddPCR. CfDNA yield and gDNA levels were comparable in both tube types and were not affected by prolonged storage of blood samples for at least 3 days in cfDNA BCTs or 6 hours in K2EDTA tubes. In addition, biospecimens collected in K2EDTA tubes and cfDNA BCTs stored for up to 3 days demonstrated highly comparable levels of mutational load across all respective cancer patient cohorts and a wide range of concentrations. Our data support the applicability of clinical oncology specimens collected and stored in cfDNA BCTs for up to 3 days for reliable cfDNA and mutation analyses.
Implementation of an Attention Mechanism Model for Facial Beauty Assessment Using Transfer Learning
Authors: Yang Chao-Tung , Wang Yu-Chieh , Lo Lun-Jou , Chiang Wen-Chung , Kuang Shih-Ku , Lin Hsiu-Hsia
Published: March 2023

An important consideration in medical plastic surgery is the evaluation of the patient’s facial symmetry. However, because facial attractiveness is a slightly individualized cognitive experience, it is difficult to determine face attractiveness manually. This study aimed to train a model for assessing facial attractiveness using transfer learning while also using the fine-grained image model to separate similar images by first learning features. In this case, the system can make assessments based on the input of facial photos. Thus, doctors can quickly and objectively treat patients’ scoring and save time for scoring. The transfer learning was combined with CNN, Xception, and attention mechanism models for training, using the SCUT-FBP5500 dataset for pre-training and freezing the weights as the transfer learning model. Then, we trained the Chang Gung Memorial Hospital Taiwan dataset to train the model based on transfer learning. The evaluation uses the mean absolute error percentage (MAPE) value. The root mean square error (RMSE) value is used as the basis for experimental adjustment and the quantitative standard for the model’s predictive. The best model can obtain 0.50 in RMSE and 18.5% average error in MAPE. A web page was developed to infer the deep learning model to visualize the predictive model.
Altered Functional Brain Network Structure between Patients with High and Low Generalized Anxiety Disorder
Authors: Qi Xuchen , Fang Jiaqi , Sun Yu , Xu Wanxiu , Li Gang
Published: March 2023

To investigate the differences in functional brain network structures between patients with a high level of generalized anxiety disorder (HGAD) and those with a low level of generalized anxiety disorder (LGAD), a resting-state electroencephalogram (EEG) was recorded in 30 LGAD patients and 21 HGAD patients. Functional connectivity between all pairs of brain regions was determined by the Phase Lag Index (PLI) to construct a functional brain network. Then, the characteristic path length, clustering coefficient, and small world were calculated to estimate functional brain network structures. The results showed that the PLI values of HGAD were significantly increased in alpha2, and significantly decreased in the theta and alpha1 rhythms, and the small-world attributes for both HGAD patients and LGAD patients were less than one for all the rhythms. Moreover, the small-world values of HGAD were significantly lower than those of LGAD in the theta and alpha2 rhythms, which indicated that the brain functional network structure would deteriorate with the increase in generalized anxiety disorder (GAD) severity. Our findings may play a role in the development and understanding of LGAD and HGAD to determine whether interventions that target these brain changes may be effective in treating GAD.
Helicobacter pylori Infection in Children: A Possible Reason for Headache?
Authors: Lupu Ancuta , Gavrilovici Cristina , Lupu Vasile Valeriu , Cianga Anca Lavinia , Cernomaz Andrei Tudor , Starcea Iuliana Magdalena , Mihai Cristina Maria , Tarca Elena , Mocanu Adriana , Fotea Silvia
Published: March 2023

(1) Background: The correlation between infection with Helicobacter pylori (H. pylori) and headache has been argued and explored for a long time, but a clear association between the simultaneous presence of the two in children has not been established yet. In this study, we aimed to explore this relationship in children from the Northeast region of Romania. (2) Methods: A retrospective study exploring the correlation between children having H. pylori infection and headache or migraine was conducted on a batch of 1757 children, hospitalized over 3 years in a pediatric gastroenterology department in Northeast Romania. (3) Results: A total of 130 children of both sexes had headache. From 130 children, 54 children (41.5%) also presented H. pylori infection. A significant association between headache and H. pylori infection (χ2; p < 0.01) was noticed. (4) Conclusions: More studies are needed on this relationship, and we emphasize the importance of further analyses, as they present great clinical importance for both prompt diagnosis and treatment.
OCT Angiography in Noninfectious Uveitis: A Description of Five Cases and Clinical Applications
Authors: Melachuri Samyuktha , Dansingani Kunal K. , Wesalo Joshua , Paez-Escamilla Manuel , Gagrani Meghal , Atta Sarah , Indermill Chad , Sahel José-Alain , Nischal Ken K. , Chhablani Jay , Errera Marie-Hélène
Published: March 2023

Background: Optical coherence tomography angiography (OCTA) is a noninvasive imaging modality used to analyze the retinochoroidal vasculature and detect vascular flow. The resulting images can be segmented to view each vascular plexus individually. While fluorescein angiography is still the gold standard for the diagnosis of posterior uveitis, it has limitations, and can be replaced by OCTA in some cases. Methods: This case series describes five patients with posterior noninfectious uveitis and their description by OCTA. Results: Cases included lupus retinopathy (n = 1) for which OCTA showed ischemic maculopathy as areas of flow deficit at the superficial and deep capillary plexus; choroidal granulomas (n = 1) with a non-detectable flow signal in the choroid; active punctate inner choroiditis and multifocal choroiditis (n = 1) with OCTA that showed active inflammatory chorioretinal lesions as non-detectable flow signals in choriocapillaris and choroid; dense type 2 inflammatory secondary neovascularization (n = 1) associated with active choroiditis; and acute posterior multifocal placoid pigment epitheliopathy (APMPPE) (n = 1) without flow abnormalities at the superficial and deep retinal plexuses but non-detectable flow at the levels of the choriocapillaris and choroid. Conclusions: Ophthalmologists can use OCTA to identify inflammatory changes in retinal and choroidal vasculature, aiding in the diagnosis, management, and monitoring of posterior uveitis.
The Impact of the IKBKG Gene on the Appearance of the Corpus Callosum Abnormalities in Incontinentia Pigmenti
Authors: Minić Snežana , Cerovac Nataša , Novaković Ivana , Gazikalović Slobodan , Popadić Svetlana , Trpinac Dušan
Published: March 2023

Incontinentia pigmenti (IP) is a rare skin disease combined with anomalies of the teeth, eyes, and central nervous system (CNS). Mutations of the IKBKG gene are responsible for IP. Among the most frequent CNS abnormalities found in IP using magnetic resonance imaging (MRI) are corpus callosum (CC) abnormalities. The aim of the study was to determine the presence of CC abnormalities, their relationship with the IKBKG mutations, and the possible presence of mutations of other genes. A group of seven IP patients was examined. Analyses of the IKBKG gene and the X-chromosome inactivation pattern were performed, as well as MRI and whole exome sequencing (WES) with the focus on the genes relevant for neurodegeneration. WES analysis showed IKBKG mutation in all examined patients. A patient who had a mutation of a gene other than IKBKG was excluded from further study. Four of the seven patients had clinically diagnosed CNS anomalies; two out of four had MRI-diagnosed CC anomalies. The simultaneous presence of IKBKG mutation and CC abnormalities and the absence of other mutations indicate that IKBKG may be the cause of CC abnormalities and should be included in the list of genes responsible for CC abnormalities.
Lane Level Positioning Method for Unmanned Driving Based on Inertial System and Vector Map Information Fusion Applicable to GNSS Denied Environments
Authors: Dai Minpeng , Li Haoyang , Liang Jian , Zhang Chunxi , Pan Xiong , Tian Yizhuo , Cao Jinguo , Wang Yuxuan
Published: March 2023

With the development of vehicle sensors, unmanned driving has become a research hotspot. Positioning is also considered to be one of the most challenging directions in this field. Aiming at the poor positioning accuracy of vehicles under GNSS denied environments, a lane-level positioning method based on inertial system and vector map information fusion is proposed. A dead reckoning model based on optical fiber IMU and odometer is established, and its positioning error is regarded as a priori information. Furthermore, a map matching model based on HMM is built up. Three validation experiments are carried out and experimental results show that the positioning error can be reduced to less than 30 cm when driving for about 7 min, which proves the effectiveness of the proposed method. Our work may provide a reference for the further improvement of positioning for unmanned driving under GNSS denied environments.
Method of 3D Voxel Prescription Map Construction in Digital Orchard Management Based on LiDAR-RTK Boarded on a UGV
Authors: Han Leng , Wang Shubo , Wang Zhichong , Jin Liujian , He Xiongkui
Published: March 2023

Precision application of pesticides based on tree canopy characteristics such as tree height is more environmentally friendly and healthier for humans. Offline prescription maps can be used to achieve precise pesticide application at low cost. To obtain a complete point cloud with detailed tree canopy information in orchards, a LiDAR-RTK fusion information acquisition system was developed on an all-terrain vehicle (ATV) with an autonomous driving system. The point cloud was transformed into a geographic coordinate system for registration, and the Random sample consensus (RANSAC) was used to segment it into ground and canopy. A 3D voxel prescription map with a unit size of 0.25 m was constructed from the tree canopy point cloud. The height of 20 trees was geometrically measured to evaluate the accuracy of the voxel prescription map. The results showed that the RMSE between tree height calculated from the LiDAR obtained point cloud and the actual measured tree height was 0.42 m, the relative RMSE (rRMSE) was 10.86%, and the mean of absolute percentage error (MAPE) was 8.16%. The developed LiDAR-RTK fusion acquisition system can generate 3D prescription maps that meet the requirements of precision pesticide application. The information acquisition system of developed LiDAR-RTK fusion could construct 3D prescription maps autonomously that match the application requirements in digital orchard management.
Artificial Neural Network for Classifying Financial Performance in Jordanian Insurance Sector
Authors: Al Omari Rania , Alkhawaldeh Rami S. , Jaber Jamil J.
Published: March 2023

Over the past few decades, financial performance has attracted researchers’ attention, especially in the insurance sector. Insurance is a tool for the growth and sustainability of both rising and developing economies. It promotes economic stability for people, organizations, and governments by taking on risk and spreading it across the market. We intend to classify insurance companies’ financial performance in Jordan’s Amman Stock Exchange (ASE). The sample size is 15 out of 22 selected insurance firms from 2008 to 2020. We apply the Multi-Layer Perceptron (MLP) model for the detection of (high/low) total asset turnover (TAT) as output, while we select the subrogation (SB), claims paid (CP), market capitalization (MC), and total shareholders’ equity (SE) as input to the MLP model. The performance of the MLP model is evaluated using different criteria, namely the false positive rate (FP rate), false negative rate (FN rate), F-measure, precision, and accuracy (ACC). The results show that MLP is efficient and performs well in multiple criterion tests through iteration growth. Based on our knowledge, the paper assesses the financial performance of Jordanian insurance firms, which has not been investigated previously. Furthermore, this study gives valuable information to regulators and policymakers to improve asset management efficiency in the insurance sector.