bluuebunny/crossref_metadata_2025 huggingface dataset:
Utilization of Targeted RNA-Seq for the Resolution of Variant Pathogenicity and Enhancement of Diagnostic Yield in Dysferlinopathy
Authors: Rufibach Laura , Berger Kiera , Chakravorty Samya , Emmons Sarah , Long Laurie , Gibson Greg , Hegde Madhuri
Published: March 2023

For inherited diseases, obtaining a definitive diagnosis is critical for proper disease management, family planning, and participation in clinical trials. This can be challenging for dysferlinopathy due to the significant clinical overlap between the 30+ subtypes of limb–girdle muscular dystrophy (LGMD) and the large number of variants of unknown significance (VUSs) that are identified in the dysferlin gene, DYSF. We performed targeted RNA-Seq using a custom gene-panel in 77 individuals with a clinical/genetic suspicion of dysferlinopathy and evaluated all 111 identified DYSF variants according to the American College of Medical Genetics and Genomics and the Association for Molecular Pathology (ACMG/AMP) guidelines. This evaluation identified 11 novel DYSF variants and allowed for the classification of 87 DYSF variants as pathogenic/likely pathogenic, 8 likely benign, while 16 variants remained VUSs. By the end of the study, 60 of the 77 cases had a definitive diagnosis of dysferlinopathy, which was a 47% increase in diagnostic yield over the rate at study onset. This data shows the ability of RNA-Seq to assist in variant pathogenicity classification and diagnosis of dysferlinopathy and is, therefore, a type of analysis that should be considered when DNA-based genetic analysis is not sufficient to provide a definitive diagnosis.
The Intersections between Food and Cultural Landscape: Insights from Three Mountain Case Studies
Authors: Fontefrancesco Michele F. , Zocchi Dauro M. , Pieroni Andrea
Published: March 2023

In the last decades, scholars from different disciplines have used the foodscape as a concept and an analytical framework to explore the intersection between landscape, people and food culture. Adopting a comparative case-study analysis, this article aims to show how a foodscape can be used as a lens to investigate cultural landscapes, specifically in mountain areas affected by fast structural socio-economic and ecological changes, identifying key tangible and intangible elements, the underpinning relationship and values, as well as the factors underlying their evolution and transformation. In this way, the article indicates this concept as a key tool for landscape management and conservation. We discuss three different and complementary approaches to the analysis of cultural landscapes, namely, from food products to landscape analysis (Albania), from food production practices to landscape analysis (Kenya) and from food-related rural architecture to landscape analysis (Italy). Overall, the research highlights how implementing a foodscape lens among the different levels of landscape analysis could contribute to the assessment, protection and promotion of local food-related resources. In so doing, it opens new research aimed at defining the limits of this heuristic instrument, where its most promising aspects of the foodscape have been explored in the article.
Target Trial Emulation Using Hospital-Based Observational Data: Demonstration and Application in COVID-19
Authors: Martinuka Oksana , Cube Maja von , Hazard Derek , Marateb Hamid Reza , Mansourian Marjan , Sami Ramin , Hajian Mohammad Reza , Ebrahimi Sara , Wolkewitz Martin
Published: March 2023

Methodological biases are common in observational studies evaluating treatment effectiveness. The objective of this study is to emulate a target trial in a competing risks setting using hospital-based observational data. We extend established methodology accounting for immortal time bias and time-fixed confounding biases to a setting where no survival information beyond hospital discharge is available: a condition common to coronavirus disease 2019 (COVID-19) research data. This exemplary study includes a cohort of 618 hospitalized patients with COVID-19. We describe methodological opportunities and challenges that cannot be overcome applying traditional statistical methods. We demonstrate the practical implementation of this trial emulation approach via clone–censor–weight techniques. We undertake a competing risk analysis, reporting the cause-specific cumulative hazards and cumulative incidence probabilities. Our analysis demonstrates that a target trial emulation framework can be extended to account for competing risks in COVID-19 hospital studies. In our analysis, we avoid immortal time bias, time-fixed confounding bias, and competing risks bias simultaneously. Choosing the length of the grace period is justified from a clinical perspective and has an important advantage in ensuring reliable results. This extended trial emulation with the competing risk analysis enables an unbiased estimation of treatment effects, along with the ability to interpret the effectiveness of treatment on all clinically important outcomes.
Severe Course of COVID-19 and Long-COVID-19 in Children: Difficulties in Diagnosis
Authors: Vasichkina Elena , Kofeynikova Olga , Fetisova Svetlana , Starshinova Anastasia Y. , Sheyanova Elizaveta , Vershinina Tatiana , Ryzhkov Anton , Skripnik Aleksey , Alekseeva Daria , Nechaeva Elizaveta , Glushkova Anzhela , Kudlay Dmitry , Pervunina Tatiana , Starshinova Anna
Published: March 2023

The question of COVID-19 and long-COVID-19 course in children remains unsolved. This infection in children, which is associated with COVID-19, can vary from asymptomatic to systemic damage of various systems. Multisystem inflammatory syndrome in children, associated with SARS-CoV-2 (MIS-C), is a serious condition in children and adolescents after experiencing COVID-19. Published data on MIS-C have indicated that the inflammation can be registered in the gastrointestinal tract (60–100%), as well as in cardiovascular (80%), nervous (29–58%), and respiratory (21–65%) systems. However, with the changing characteristics of SARS-CoV-2, the manifestations of COVID-19 and long-COVID-19 in children have also been changing. Currently, there is no clear understanding of the development of severe COVID-19 and MIS-C in children, especially after being exposed to patients with COVID-19. We presented two new clinical courses of multisystem inflammatory syndrome in children with severe multisystem damage after close contact to relatives with COVID-19 or long-COVID-19. Thus, high-risk children, who are positive for SARS-CoV-2 infection after contact with COVID-19 patients, should be clinically managed during the first few months. The identification of the disease complexity requires the involvement of neurologists, cardiologists, and other specialists.
Study of the Plastic Behavior of Rough Bearing Surfaces Using a Half-Space Contact Model and the Fatigue Life Estimation According to the Fatemi–Socie Model
Authors: Foko Foko Flavien , Rüth Lukas , Koch Oliver , Sauer Bernd
Published: March 2023

A multiscale approach for the fatigue life estimation of rolling bearings is presented in this paper and applied to inner rings of cylindrical roller bearings of the type NU208. The forces acting in the rolling contact are determined from system-oriented modeling at the macro level. The microscale contact simulations are carried out in a half-space contact model. The stresses on the inner ring are determined and used in the local fatigue approach, according to Fatemi–Socie, for fatigue life estimation. Four surface variants were investigated, one ideally smooth surface and three real (rough) surfaces. The three rough surface variants used different finishing processes: fine ground, hard turned, and rough ground were produced. A load case with a maximum pressure of 2.4 GPa in the roller-inner ring contact was investigated. In addition to the fatigue life estimation, the plasticity behavior (surface topography, resulting contact pressure, and residual stress) of the three manufactured surfaces stood in the focus of the work. As the comparison between experimental and simulated results confirms, good predictions can be made with the simulation model.
Frictional Properties of Two-Dimensional Nanomaterials as an Additive in Liquid Lubricants: Current Challenges and Potential Research Topics
Authors: Castellanos-Leal Edgar Leonardo , Osuna-Zatarain Angel , Garcia-Garcia Alejandra
Published: March 2023

This paper reports on the trend of studying and applying two-dimensional materials in tribology. Two-dimensional materials have improved the ability of lubricants when used as additives to reduce wear between surfaces through the formation of protective layers by sliding on metal surfaces. The morphology and chemical nature of 2D materials are among the important factors that influence their dispersion in the lubricant medium and determine the final performance of the lubricant for various applications. The mentioned materials in this work are h-BN, graphene, graphene oxide, and MoS2 as part of the transition metal dichalcogenides. The most studied material to date is graphene and its analogs, such as graphene oxide, which, under controlled conditions, can present superlubricity, with COF values less than 0.01. Some methodologies applied to modify two-dimensional materials and examples of the application and characterization of their performance in tribology are mentioned. This review also shows the benefits of using 2D nanomaterials and the synergy generated when two or more of them are combined to not only achieve superlubricity but also improve corrosion resistance and mechanical properties at the interfaces found in contact.
Recent Advances on PEO-PCL Block and Graft Copolymers as Nanocarriers for Drug Delivery Applications
Authors: Chountoulesi Maria , Selianitis Dimitrios , Pispas Stergios , Pippa Natassa
Published: March 2023

Poly(ethylene oxide)-poly(ε-caprolactone) (PEO-PCL) is a family of block (or graft) copolymers with several biomedical applications. These types of copolymers are well-known for their good biocompatibility and biodegradability properties, being ideal for biomedical applications and for the formation of a variety of nanosystems intended for controlled drug release. The aim of this review is to present the applications and the properties of different nanocarriers derived from PEO-PCL block and graft copolymers. Micelles, polymeric nanoparticles, drug conjugates, nanocapsules, and hybrid polymer-lipid nanoparticles, such as hybrid liposomes, are the main categories of PEO-PCL based nanocarriers loaded with different active ingredients. The advantages and the limitations in preclinical studies are also discussed in depth. PEO-PCL based nanocarriers could be the next generation of delivery systems with fast clinical translation. Finally, current challenges and future perspectives of the PEO-PCL based nanocarriers are highlighted.
Study on the Evolution of Physical Parameters and Dynamic Compression Mechanical Properties of Granite after Different Heating and Cooling Cycles
Authors: Zhang Hongzhong , Huang Linqi , Li Xibing , Hu Xingmiao , Wu Yangchun
Published: March 2023

The study of the evolution law of basic physical parameters and dynamic compression performance of deep granite under the environment of the heating-cooling cycle is of great significance for the stability evaluation of deep underground engineering and the development of deep resources. In this study, heating-cooling cycle tests and dynamic compression tests were conducted on a large number of fine-grained granite specimens with heating temperatures from 200 to 600 °C and times from one to twenty times using a box-type high-temperature muffle furnace and Hopkinson pressure bar (SHPB) test system, and the evolution law of basic physical parameters and dynamic compression mechanical properties of fine-grained granite were studied using theoretical and fitting analysis. The test results showed that: the changes of the basic physical parameters of granite have obvious temperature effect; 600 °C is a threshold value for the changes of each physical parameter of granite; the sensitivity of each physical parameter to the number of heating and cooling cycles is small before 600 °C; and the sensitivity of each physical parameter to the number of heating and cooling cycles significantly increases at 600 °C. The dynamic compressive strength and elastic modulus of granite decreased with the increase in heating and cooling cycles, and the maximum decrease rate was 89.1% and 85.9%, respectively, and the strain rate linearly increased with the increase in heating and cooling cycles, and the maximum strain rate was 123 s−1. The temperature, the number of heating and cooling cycles, and the impact air pressure, all had significant effects on the damage mode and crushing degree of granite.
Modification Effect of Ca(OH)2 on the Carbonation Resistance of Fly Ash-Metakaolin-Based Geopolymer
Authors: Lv Yigang , Qiao Jie , Han Weiwei , Pan Bei , Jin Xiafei , Peng Hui
Published: March 2023

Compared with Portland cement, geopolymers have poor carbonization resistance, which will greatly limit the application their application. To improve the carbonization resistance of geopolymers, firstly, the carbonization behavior of the fly ash-metakaolin-based geopolymer was studied through accelerated carbonization tests. Secondly, different amounts of Ca(OH)2 were introduced into the composite system, and the modification effect of the carbonization resistance of the modified geopolymer was studied. Finally, the modification effect of Ca(OH)2 on the fly ash-metakaolin-based geopolymers was analyzed, and the modification mechanism was explored. It was found that adding Ca(OH)2 to the fly ash-metakaolin-based geopolymer could significantly improve its initial compressive strength, but its strength after carbonization remained basically unchanged; meanwhile, the compressive strength of the terpolymer after carbonization clearly decreased after adding Ca(OH)2. Compared with ordinary Portland cement, the carbonization rate of fly ash-metakaolin-based geopolymer is faster, and the addition of Ca(OH)2 can inhibit the development of its carbonization depth. With increased carbonization age, the alkalinity of the geopolymer decreased, and the addition of Ca(OH)2 inhibited the decrease in the alkalinity of the geopolymer. The addition of Ca(OH)2 improved the microstructure of the geopolymers, the pore structure became denser, and the pore size became smaller size after carbonization. The hydration products of fly ash-metakaolin-based geopolymer are mainly amorphous silicaluminate gel and C–S–H gel, and Ca(OH)2 forms in the hydration products of terpolymer with the incorporation of Ca(OH)2, which is conducive to improving the carbonization resistance. In summary, Ca(OH)2 can play a good role in modifying the carbonization resistance of fly ash-metakaolin-based geopolymers.
Unusual Acid Sites in LSX Zeolite: Formation Features and Physico-Chemical Properties
Authors: Leonova Aleksandra A. , Yashnik Svetlana A. , Paukshtis Evgeny A. , Mel’gunov Maksim S.
Published: March 2023

The advanced approach for the preparation of the NH4 form of highly crystalline LSX zeolite under gentle drying conditions (40 °C, membrane pump dynamic vacuum) is discussed. Decationization of this form at moderate temperatures led to the formation of Brønsted acid sites (BASs), whose concentration and strength were characterized by IR spectroscopy. It was found that a maximum concentration of three BASs per unit cell can be achieved at 200 °C prior to the initiation of zeolite structure degradation. The proton affinity of BASs is unusual, and aspires 1240 kJ/mol, which is significantly higher compared to faujasites with higher moduli. The increase in temperature of the heat treatment (up to 300 °C) resulted in thermal decomposition of BASs and the manifestation of amorphous phase with corresponding Lewis acid sites (LASs) as well as terminal Si–OH groups. Both the destruction of BASs and formation of the LAS-containing amorphous phase are the key reasons for the significant decrease in the adsorption capacity in the micropore region revealed for the sample decationized at 300 °C.
Estimation of Shear Modulus and Hardness of High-Entropy Alloys Made from Early Transition Metals Based on Bonding Parameters
Authors: Temesi Ottó , Varga Lajos K. , Li Xiaoqing , Vitos Levente , Chinh Nguyen Q.
Published: March 2023

The relationship between the tendencies towards rigidity (measured by shear modulus, G) and hardness (measured by Vickers hardness, HV) of early transition metal (ETM)-based refractory high-entropy alloys (RHEA) and bond parameters (i.e., valence electron concentration (VEC), enthalpy of mixing (ΔHmix)) was investigated. These bond parameters, VEC and ΔHmix, are available from composition and tabulated data, respectively. Based on our own data (9 samples) and those available from the literatures (47 + 27 samples), it seems that for ETM-based RHEAs the G and HV characteristics have a close correlation with the bonding parameters. The room temperature value of G and HV increases with the VEC and with the negative value of ΔHmix. Corresponding equations were deduced for the first time through multiple linear regression analysis, in order to help design the mechanical properties of ETM refractory high-entropy alloys.
Enhanced Filtration Efficiency of Natural Materials with the Addition of Electrospun Poly(vinylidene fluoride-co-hexafluoropropylene) Fibres
Authors: Filipič Gregor , Pirker Luka , Krajnc Anja Pogačnik , Ješelnik Marjan , Remškar Maja
Published: March 2023

Pollutants and infectious diseases can spread through air with airborne droplets and aerosols. A respiratory mask can decrease the amount of pollutants we inhale and it can protect us from airborne diseases. With the onset of the COVID-19 pandemic, masks became an everyday item used by a lot of people around the world. As most of them are for a single use, the amount of non-recyclable waste increased dramatically. The plastic from which the masks are made pollutes the environment with various chemicals and microplastic. Here, we investigated the time- and size-dependent filtration efficiency (FE) of aerosols in the range of 25.9 to 685.4 nm of five different natural materials whose FE was enhanced using electrospun poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF) fibres. A scanning electron microscope (SEM) was used to determine the morphology and structure of the natural materials as well as the thickness of the PVDF fibres, while the phase of the electrospun fibres was determined by Raman spectroscopy. A thin layer of the electrospun PVDF fibres with the same grammage was sandwiched between two sheets of natural materials, and their FE increased up to 80%. By varying the grammature of the electrospun polymer, we tuned the FE of cotton from 82.6 to 99.9%. Thus, through the optimization of the grammage of the electrospun polymer, the amount of plastic used in the process can be minimized, while achieving sufficiently high FE.
Copper Ferrite Nanoparticles Synthesized Using Anion-Exchange Resin: Influence of Synthesis Parameters on the Cubic Phase Stability
Authors: Saikova Svetlana , Pavlikov Aleksandr , Karpov Denis , Samoilo Aleksandr , Kirik Sergey , Volochaev Mikhail , Trofimova Tatyana , Velikanov Dmitry , Kuklin Artem
Published: March 2023

Copper ferrite is of great interest to researchers as a material with unique magnetic, optical, catalytic, and structural properties. In particular, the magnetic properties of this material are structurally sensitive and can be tuned by changing the distribution of Cu and Fe cations in octahedral and tetrahedral positions by controlling the synthesis parameters. In this study, we propose a new, simple, and convenient method for the synthesis of copper ferrite nanoparticles using a strongly basic anion-exchange resin in the OH form. The effect and possible mechanism of polysaccharide addition on the elemental composition, yield, and particle size of CuFe2O4 are investigated and discussed. It is shown that anion-exchange resin precipitation leads to a mixture of unstable cubic (c-CuFe2O4) phases at standard temperature and stable tetragonal (t-CuFe2O4) phases. The effect of reaction conditions on the stability of c-CuFe2O4 is studied by temperature-dependent XRD measurements and discussed in terms of cation distribution, cooperative Jahn–Teller distortion, and Cu2+ and oxygen vacancies in the copper ferrite lattice. The observed differences in the values of the saturation magnetization and coercivity of the prepared samples are explained in terms of variations in the particle size and structural properties of copper ferrite.
Fuzzy Triple Controlled Metric like Spaces with Applications
Authors: Saleem Naeem , Furqan Salman , Abuasbeh Kinda , Awadalla Muath
Published: March 2023

In this article, we introduce the concept of a fuzzy triple controlled metric like space in the sense that the self distance may not be equal to one. We have used three functions in our space that generalize fuzzy controlled rectangular, extended fuzzy rectangular, fuzzy b–rectangular and fuzzy rectangular metric like spaces. Various examples are given to justify our definitions and results. As for the topological aspect, we prove a fuzzy triple controlled metric like space is not Hausdorff. We also apply our main result to solve the uniqueness of the solution of a fractional differential equation.
Neimark–Sacker Bifurcation of a Discrete-Time Predator–Prey Model with Prey Refuge Effect
Authors: Hong Binhao , Zhang Chunrui
Published: March 2023

In this paper, we deduce a predator–prey model with discrete time in the interior of R+2 using a new discrete method to study its local dynamics and Neimark–Sacker bifurcation. Compared with continuous models, discrete ones have many unique properties that help to understand the changing patterns of biological populations from a completely new perspective. The existence and stability of the three equilibria are analyzed, and the formation conditions of Neimark–Sacker bifurcation around the unique positive equilibrium point are established using the center manifold theorem and bifurcation theory. An attracting closed invariant curve appears, which corresponds to the periodic oscillations between predators and prey over a long period of time. Finally, some numerical simulations and their biological meanings are given to reveal the complex dynamical behavior.
Exploring the Most Effective Apical Seal for Contemporary Bioceramic and Conventional Endodontic Sealers Using Three Obturation Techniques
Authors: Akhtar Hira , Naz Farah , Hasan Arshad , Tanwir Anum , Shahnawaz Danish , Wahid Umair , Irfan Fariha , Ahmed Muhammad Adeel , Almadi Khalid H. , Alkahtany Mazen F. , Abduljabbar Tariq , Vohra Fahim
Published: March 2023

Background and Objective: Despite a plethora of studies conducted to date, researchers continue to investigate the best sealer and obturation technique combinations. The aim of this study is to compare the apical seal provided by two bioceramic sealers (Endoseal and Endosequence) with that provided by a calcium hydroxide sealer (Sealapex), and to evaluate the effect of different obturation techniques (cold lateral condensation, continuous wave compaction and single cone) on the apical seal under a stereomicroscope. Materials and Methods: A total of 110 single-rooted mandibular premolar teeth were decoronated, cleaned and shaped using the Endosequence filing system to tip size 30/0.04 taper. Canals were irrigated with 5.25% NaOCl and 17% EDTA. The samples were randomly divided into 11 groups (9 experimental and 2 control groups) according to the designated sealer and technique. Samples were stored in an incubator for 7 days at 37 °C under 100% humidity. Samples were coated with nail varnish except for apical 2 mm and vertically placed in 0.2% rhodamine B dye solution for 48 h. Samples were split longitudinally and viewed under a stereomicroscope at 40× magnification. Results: Insignificant results were obtained between obturation techniques (p = 0.499) whereas statistically significant results were attained based on the type of endodontic sealer (p < 0.001). The overall lowest mean apical microleakage and best sealing ability was demonstrated by Sealapex (2.59 ± 1.20 mm) and amongst techniques by continuous wave compaction (3.90 ± 2.51 mm). Conclusions: Endosequence produced the best apical seal with the continuous wave compaction technique, whereas Endoseal did so with the bioceramic-coated single-cone technique. For the Sealapex sealer, the most effective apical seal was observed using cold lateral condensation. The quality and effectiveness of apical seal differed with the type of endodontic sealer and obturation technique used, and vice versa.
The Immunosuppressive Niche Established with a Curcumin-Loaded Electrospun Nanofibrous Membrane Promotes Cartilage Regeneration in Immunocompetent Animals
Authors: Zhang Yu , Cai Renzhong , Li Jun , Wu Xu
Published: March 2023

Inflammatory cells mount an immune response against in vitro engineered cartilage implanted into immunocompetent animals, consequently limiting the usage of tissue-engineered cartilage to repair cartilage defects. In this study, curcumin (Cur)—an anti-inflammatory agent—was mixed with poly(lactic-co-glycolic acid) (PLGA) to develop a Cur/PLGA nanofibrous membrane with nanoscale pore size and anti-inflammatory properties. Fourier-transform infrared spectroscopy and high-performance liquid chromatography analyses confirmed the successful loading of Cur into the Cur/PLGA nanofibrous membrane. The results of the in vitro assay demonstrated the sustained release kinetics and enhanced stability of Cur in the Cur/PLGA nanofibrous membrane. Western blotting and enzyme-linked immunosorbent assay analyses revealed that the Cur/PLGA nanofibrous membrane significantly downregulated the expression of inflammatory cytokines (IL-1β, IL-6, and TNF-α). A chondrocyte suspension was seeded into a porous PLGA scaffold, and the loaded scaffold was cultured for 3 weeks in vitro to engineer cartilage tissues. The cartilage was packed with the in vitro engineered Cur/PLGA nanofibrous membrane and subcutaneously implanted into rats to generate an immunosuppressive niche. Compared with those in the PLGA-implanted and pure cartilage (without nanofibrous membrane package)-implanted groups, the cartilage was well preserved and the inflammatory response was suppressed in the Cur/PLGA-implanted group at weeks 2 and 4 post-implantation. Thus, this study demonstrated that packaging the cartilage with the Cur/PLGA nanofibrous membrane effectively generated an immunosuppressive niche to protect the cartilage against inflammatory invasion. These findings enable the clinical translation of tissue-engineered cartilage to repair cartilage defects.
Fabrication and Characterization of SiC-reinforced Aluminium Matrix Composite for Brake Pad Applications
Authors: Chatterjee Arpita , Sen Soumyadeep , Paul Subhodeep , Roy Pallab , Seikh Asiful H. , Alnaser Ibrahim A. , Das Kalyan , Sutradhar Goutam , Ghosh Manojit
Published: March 2023

The wear debris from conventional brake pads is a growing source of environmental contamination that often leads to life-threatening diseases for human beings. Though the emerging organic brake pads show potential to serve as an eco-friendly alternative, their mechanical and tribological properties are not adequate to withstand the demands of high-wear resistance of a functioning braking system under regular use. Metal matrix composites have served as an optimal solution with minimal environmental pollution and appreciable physical properties. Owing to the popularity of aluminium metal matrix composites, the present study is based on the fabrication and characterization of SiC-reinforced LM6 alloy through stir casting methodologies for evaluating its worthiness in application as a brake pad material. Microstructural, compositional, and phase characterizations were executed through optical micrography, X-ray diffraction, and energy-dispersive X-ray spectroscopy analysis. Although mechanical properties were evaluated through surface hardness investigation, parallel thermal properties were estimated through thermal conductivity evaluation. Finally, the execution of tribological analysis and precise microstructural observations of wear track at ambient and elevated temperatures helped in establishing the datum that the fabricated metal matrix composite (MMC) is a reliable brake pad material alternative.
Material Behavior of High-Strength Low-Alloy Steel (HSLA) WAAM Walls in Construction
Authors: Jahns Hendrik , Unglaub Julian , Müller Johanna , Hensel Jonas , Thiele Klaus
Published: March 2023

Additive manufacturing with steel offers new opportunities for the construction sector. In particular, direct energy deposition (DED) processes such as Wire Arc Additive Manufacturing (WAAM or DED-Arc), are able to create large structures with a high degree of geometric freedom, like force-flow-optimized steel nodes and frameworks, as well as truss structures. By using high-strength steel, manufacturing times can be shortened because less material has to be applied. In order to enable the usage of WAAM components in the construction industry, profound knowledge of the material behavior is necessary. Based on reliable process parameters, extensive experimental and numerical investigations are carried out to characterize the influence of layer orientation and overhang angle on the mechanical parameters of WAAM high-strength low-alloy steel (HSLA) walls. The results have been compared to HSLA steel sheet material. It is shown that comparable characteristics exist for Young’s modulus E, yield strength Rp,0.2 and tensile strength Rm with regard to civil engineering applications. The influence of the loading direction on the material level is similar. Only the yield strength shows a slight dependence on the layer orientation for WAAM walls (difference 4.5%). The overhang angle has no influence on the material parameters.
Biomarkers Correlated with Tuberculosis Preventive Treatment Response: A Systematic Review and Meta-Analysis
Authors: Zhang Haoran , Sun Zuyu , Liu Yi , Wei Rongrong , Che Nanying
Published: March 2023

Background: There is a need to identify alternative biomarkers to predict tuberculosis (TB) preventive treatment response because observing the incidence decline renders a long follow-up period. Methods: We searched PubMed, Embase and Web of Science up to 9 February 2023. The biomarker levels during preventive treatment were quantitatively summarized by means of meta-analysis using the random-effect model. Results: Eleven eligible studies, published during 2006–2022, were included in the meta-analysis, with frequently heterogeneous results. Twenty-six biomarkers or testing methods were identified regarding TB preventive treatment monitoring. The summarized standard mean differences of interferon-γ (INF-γ) were −1.44 (95% CI: −1.85, −1.03) among those who completed preventive treatment (τ2 = 0.21; I2 = 95.2%, p < 0.001) and −0.49 (95% CI: −1.05, 0.06) for those without preventive treatment (τ2 = 0.13; I2 = 82.0%, p < 0.001), respectively. Subgroup analysis showed that the INF-γ level after treatment decreased significantly from baseline among studies with high TB burden (−0.98, 95% CI: −1.21, −0.75) and among those with a history of Bacillus Calmette–Guérin vaccination (−0.87, 95% CI: −1.10, −0.63). Conclusions: Our results suggested that decreased INF-γ was observed among those who completed preventive treatment but not in those without preventive treatment. Further studies are warranted to explore its value in preventive treatment monitoring due to limited available data and extensive between-study heterogeneity.
Gut Microbiota–Brain Axis in Regulation of Feeding Behavior
Authors: Fetissov Sergueï O.
Published: March 2023

The survival of microorganisms inhabiting the intestinal tract depends on the nutrients provided by the host, with the latter obtaining them through food intake. It is hence not surprising that the co-evolution of gut bacteria and their hosts, including humans, shaped intrinsic interactions between their respective metabolisms with an impact on host feeding behavior. Understanding molecular pathways underlying such interactions may aid in the development of new therapeutic approaches for several pathological conditions accompanied by altered feeding behavior. A Special Issue titled “Gut Microbiota–Brain Axis in Regulation of Feeding Behavior” contributes to this topic of research, with eight papers covering its various aspects such as autoprobiotics, metabolic diseases and anorexia.
Hybrid Finite-Discrete Element Modeling of the Mode I Tensile Response of an Alumina Ceramic
Authors: Zheng Jie , Li Haoyang , Hogan James D.
Published: March 2023

We have developed a three-dimensional hybrid finite-discrete element model to investigate the mode I tensile opening failure of alumina ceramic. This model implicitly considers the flaw system in the material and explicitly shows the macroscopic failure patterns. A single main crack perpendicular to the loading direction is observed during the tensile loading simulation. Some fragments appear near the crack surfaces due to crack branching. The tensile strength obtained by our model is consistent with the experimental results from the literature. Once validated with the literature, the influences of the distribution of the flaw system on the tensile strength and elastic modulus are explored. The simulation results show that the material with more uniform flaw sizes and fewer big flaws has stronger tensile strength and higher elastic modulus.
Solid-Liquid Triboelectric Nanogenerator Based on Vortex-Induced Resonance
Authors: Li Xiaowei , Zhang Di , Zhang Dan , Li Zhongjie , Wu Hao , Zhou Yuan , Wang Biao , Guo Hengyu , Peng Yan
Published: March 2023

Energy converters based on vortex-induced vibrations (VIV) have shown great potential for harvesting energy from low-velocity flows, which constitute a significant portion of ocean energy. However, solid-solid triboelectric nanogenerators (TENG) are not wear-resistant in corrosive environments. Therefore, to effectively harvest ocean energy over the long term, a novel solid-liquid triboelectric nanogenerator based on vortex-induced resonance (VIV-SL-TENG) is presented. The energy is harvested through the resonance between VIV of a cylinder and the relative motions of solid-liquid friction pairs inside the cylinder. The factors that affect the output performance of the system, including the liquid mass ratio and the deflection angle of the friction plates, are studied and optimized by establishing mathematical models and conducting computational fluid dynamics simulations. Furthermore, an experimental platform for the VIV-SL-TENG system is constructed to test and validate the performance of the harvester under different conditions. The experiments demonstrate that the energy harvester can successfully convert VIV energy into electrical energy and reach maximum output voltage in the resonance state. As a new type of energy harvester, the presented design shows a promising potential in the field of ‘blue energy’ harvesting.
Pool Boiling Heat Transfer Characteristics of New and Recycled Alumina Nanofluids
Authors: Ajeeb Wagd , Murshed S. M. Sohel
Published: March 2023

This paper reports an experimental investigation of the heat transfer features of new and recycled Alumina (Al2O3) nanofluids (NFs) in the pool boiling (PB) system. The mixture of ethylene glycol (EG) and distilled water (DW) is selected as the base fluid (BF), and NFs samples of two low concentrations (0.01 and 0.05 vol.%) of Al2O3 nanoparticles were prepared. Furthermore, the characteristics of the prepared NFs are evaluated to investigate the heat transfer performance as well as the reusability of the NFs for long-term applications and recycling consideration. Although there have been a large number of boiling studies with NFs, the current study is the first of its kind that addresses the mentioned operation conditions of recycling NF samples. The results are compared with the relevant BF in terms of properties, critical heat flux (CHF), burnout heat flux (BHF), and the convection coefficient of the Al2O3 NFs in the PB system. The results showed good enhancements in both CHF and BHF of these NFs yielding up to 60% and 54% for BHF at 0.05 vol.%, respectively. The reusage of the previously used (recycled) Al2O3 NF showed a considerable increase in heat transfer performance compared to base fluids but slightly lower than the newly prepared one. The results of the reused nanofluids demonstrate the great prospects of their recyclability in heat transfer systems and processes such as in pool boiling.
Synthesis of Wrinkle-Free Metallic Thin Films in Polymer by Interfacial Instability Suppression with Nanoparticles
Authors: Jalali-Mousavi Maryam , Cheng Samuel Kok Suen , Sheng Jian
Published: March 2023

Synthesis of a smooth conductive film over an elastomer is vital to the development of flexible optics and wearable electronics, but applications are hindered by wrinkles and cracks in the film. To date, a large-scale wrinkle-free film in an elastomer has yet to be achieved. We present a robust method to fabricate wrinkle-free, stress-free, and optically smooth thin film in elastomer. Targeting underlying mechanisms, we applied nanoparticles between the film and elastomer to jam the interface and subsequently suppress interfacial instabilities to prevent the formation of wrinkles. Using polydimethylsiloxane (PDMS) and parylene-C as a model system, we have synthesized large-scale (>10 cm) wrinkle-free Al film over/in PDMS and demonstrated the principle of interface jamming by nanoparticles. We varied the jammer layer thickness to show that, as the layer exceeds a critical thickness (e.g., 150 nm), wrinkles are successfully suppressed. Nano-indentation experiments revealed that the interface becomes more elastic and less viscoelastic with respect to the jammer thickness, which further supports our assertion of the wrinkle suppression mechanism. Since the film was embedded in a polymer matrix, the resultant film was highly deformable, elastic, and optically smooth with applications for deformable optical sensors and actuators.
Identifying the Associations of Nightly Fasting Duration and Meal Timing with Type 2 Diabetes Mellitus Using Data from the 2016–2020 Korea National Health and Nutrition Survey
Authors: Kwak Junkyung , Jang Kyeong-A , Kim Haeng-Ran , Kang Min-Sook , Lee Kyung Won , Shin Dayeon
Published: March 2023

Nightly fasting duration and meal timing are associated with metabolic disorders. This study aimed to investigate the relationships of nightly fasting duration and meal timing with type 2 diabetes mellitus (T2DM) using data from the 2016–2020 Korea National Health and Nutrition Survey. A total of 22,685 adults ≥ 19 years were included in this study. Nightly fasting duration was calculated by subtracting the interval between the day’s first and last meal eating times from 24 h. The meal timing were analyzed using various parameters, including the times of the first and last eating episodes and the percentage of energy intake during the morning (05:00 to 9:00 a.m.), evening (06:00 to 09:00 p.m.), and night (after 09:00 p.m.). Men who fasted nightly for ≥ 12 h had lower odds of T2DM (odds ratio (OR): 0.86; 95% confidence interval (CI): 0.75–0.99) than those who fasted for < 12 h. Individuals who had their last meal after 09:00 p.m. had higher odds of T2DM (OR: 1.19, 95% CI: 1.03–1.38, men; OR: 1.19, 95% CI: 1.01–1.40, women). Additionally, the percentage of energy intake during the evening was associated with increased odds of T2DM (OR: 1.41, 95% CI: 1.08–1.84, men; OR: 1.32, 95% CI: 1.02–1.70, women). These findings emphasize the importance of nightly fasting duration and meal timing in modulating the risk of T2DM among Korean adults.
Hidden and Rare Food Allergens in Pediatric Age
Authors: Tomei Leonardo , Muraro Antonella , Giovannini Mattia , Barni Simona , Liccioli Giulia , Paladini Erika , Sarti Lucrezia , Pessina Benedetta , Skypala Isabel , Novembre Elio , Mori Francesca
Published: March 2023

In food allergy management, the avoidance of the allergen that caused the reaction plays a fundamental role. Nevertheless, that can be thwarted in case of accidental exposure to a rare or hidden allergen, causing the adoption of a monotonous diet and a consequent reduction in the quality of life of the patient and their family. The identification of a rare and hidden allergen is an important diagnostic challenge, taking into account that a significant proportion of all food reactions is in reality due to them. The aim of the present review is to provide the pediatric allergist an overview of the possible sources of rare and hidden food allergens, taking into consideration the routes of exposure to these potential allergens with the main examples published in the scientific literature and the distinction between types of direct or cross-contamination. The identification of the allergen responsible for the reaction and the provision of a dietary advice customized for the specific individual’s dietary habits is essential to improve quality of life of the familiar nucleus and to reduce the risk of further allergic reactions.
A Qualitative Process Evaluation of Participant Experiences in a Feasibility Randomised Controlled Trial to Reduce Indulgent Foods and Beverages
Authors: Madigan Claire Deborah , Hill Andrew J. , Caterson Ian Douglas , Burk Jessica , Hendy Chelsea , Chalkley Anna
Published: March 2023

There is a growing interest in the effects of ultra-processed/energy-dense nutrient-poor foods on health outcomes, and few interventions to reduce their consumption have been tested. We tested a simple intervention to help people reduce the indulgences they consume (energy-dense nutrient-poor (EDNP) foods). Herein, we report the qualitative findings to understand how participants reduced their consumption by exploring intervention fidelity and the factors affecting consumption. We conducted a qualitative descriptive study of 23 adults who had taken part in a feasibility randomised controlled trial that asked participants to say no to seven indulgences/week and record what they said no to. Data were collected using face-to-face semi-structured interviews and analysed thematically. A total of 23 adults with an average BMI of 30.8 kg/m2 took part. Participants liked the term indulgence, as they could apply it to their normal dietary intake and make small changes. They found self-monitoring what they said no to helpful and reported that emotional eating and habits affected consumption. They had difficulty overcoming these. As most people are consuming too many foods that are EDNP, this simple intervention of “Say No” seven times/week has the potential to be developed as a public health campaign.
The Influence of Polishing on the Mechanical Properties of Zirconia—A Systematic Review
Authors: Liu Xiaoyun , Aarts John , Ma Sunyoung , Choi Joanne
Published: March 2023

Purpose: To systematically review studies that investigated the consequences of various polishing protocols on the mechanical properties of zirconia. The effects on the roughness and crystalline phase transformation were also evaluated. Materials and methods: The Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) was followed. The electronic searches were conducted via OVID MEDLINE (R) and Scopus for publications between 1996 and August 2022. The search strategy was limited to full texts in the English language and in vitro studies. The influences on flexural strength, hardness, fracture strength, fracture toughness, wear resistance, roughness and phase transformation were collected. Various methodologies to measure these properties were also outlined and compared. The risk of bias for included studies was evaluated according to a modified Consolidated Standards of Reporting Trials (CONSORT) checklist. Results: After removing duplicates, the systematic search identified a total of 419 studies. Nineteen studies satisfied the inclusion criteria and were selected for final analysis. Fifteen of the included studies observed the changes in surface roughness along with the mechanical properties and ten studies detected the tetragonal (T) to monoclinic (M) phase transformation. Eight studies also investigated the change in properties after polishing the ground surface. Testing parameters were not consistent among studies due to the varying methods. Conclusions: To a certain extent, polishing influences the strength, hardness, toughness and wear resistance. The damage in some mechanical properties, as well as the roughened surface, from grinding can be restored via an appropriate polishing treatment. The polishing process itself barely induces the transition from the tetragonal to monoclinic phase of zirconia, while this commonly occurs after grinding. If the subsequent polishing is adequate, the transformed monoclinic phase can be eliminated with the removal of the outermost surface layer. In dentistry, polishing is an imperative step to maintain the superior functions and service life of zirconia for patients.
The On/Off History of Hydrogen in Medicine: Will the Interest Persist This Time Around?
Authors: LeBaron Tyler W. , Ohno Kinji , Hancock John T.
Published: March 2023

Over 2000 publications including more than 100 human studies seem to indicate that humans have only recently benefited from or known about the medical effects of H2 within the past 15 years. However, we have unknowingly benefited from H2 since the dawn of time, from H2-producing bacteria to the use of naturally occurring hydrogen-rich waters. Moreover, the first writings on the therapeutic effects of H2 date to around 1793. Since then, papers appeared sporadically in the literature every few decades but never exploded until Ohsawa et al. again demonstrated hydrogen’s therapeutic effects in 2007. This landmark paper appears to have been the spark that ignited the medical interest in hydrogen. Although H2 was used in the 1880s to locate intestinal perforations, in the 1940s in deep sea diving, and in the 1960s to measure blood flow, H2 was largely viewed as biologically inert. This review highlights the history of hydrogen in the genesis/evolution of life and its medicinal and non-medicinal use in humans. Although hydrogen medicine has a long and erratic history, perhaps future history will show that, this time around, these 15 years of ignited interest resulted in a self-sustaining explosion of its unique medical effects.
Engineered Mesenchymal Stem Cells Over-Expressing BDNF Protect the Brain from Traumatic Brain Injury-Induced Neuronal Death, Neurological Deficits, and Cognitive Impairments
Authors: Choi Bo Young , Hong Dae Ki , Kang Beom Seok , Lee Si Hyun , Choi Seunghyuk , Kim Hyo-Jin , Lee Soon Min , Suh Sang Won
Published: March 2023

Traumatic brain injury (TBI) causes transitory or permanent neurological and cognitive impairments, which can intensify over time due to secondary neuronal death. However, no therapy currently exists that can effectively treat brain injury following TBI. Here, we evaluate the therapeutic potential of irradiated engineered human mesenchymal stem cells over-expressing brain-derived neurotrophic factor (BDNF), which we denote by BDNF-eMSCs, in protecting the brain against neuronal death, neurological deficits, and cognitive impairment in TBI rats. BDNF-eMSCs were administered directly into the left lateral ventricle of the brain in rats that received TBI damage. A single administration of BDNF-eMSCs reduced TBI-induced neuronal death and glial activation in the hippocampus, while repeated administration of BDNF-eMSCs reduced not only glial activation and delayed neuronal loss but also enhanced hippocampal neurogenesis in TBI rats. In addition, BDNF-eMSCs reduced the lesion area in the damaged brain of the rats. Behaviorally, BDNF-eMSC treatment improved the neurological and cognitive functions of the TBI rats. The results presented in this study demonstrate that BDNF-eMSCs can attenuate TBI-induced brain damage through the suppression of neuronal death and increased neurogenesis, thus enhancing functional recovery after TBI, indicating the significant therapeutic potential of BDNF-eMSCs in the treatment of TBI.