bluuebunny/crossref_metadata_2025 huggingface dataset:
Policy Development on Upskilling/Reskilling Older Population Care Staff in China
Authors: Hung Jason
Published: August 2022

Mainland China has been concerned about the national growth rate of older adults aged 60 or above. The rapid growth of the cohort of older adults will significantly burden the Chinese healthcare system as they are at higher risk of suffering from chronic illnesses and functional disabilities. In geriatrics, aged populations often endure a wide range of diseases, dysfunctions, and cognitive impairment, so the corresponding healthcare services needed for them are substantial. The rise in the older adults’ life expectancy has compounded the burden of the healthcare system in mainland China in the long term. In this narrative essay, it is important to discuss how the state should assume a higher share of relevant responsibilities, by assessing how Chinese policymaking has been transformed to better satisfy the older population’s care and healthcare needs in mainland China. It is also pivotal to focus on analysing relevant Chinese policy development within the most recent dozen years to address how China’s state and local governments have been progressing in promptly providing health and older population care services to older Chinese adults. Because of the supply shortage and low quality of older population caregivers and alternative professionals, it is necessary to discuss and highlight the need to reskill or upskill relevant caregivers. As the trend of rural-to-urban labour migration continues, working adults of rural origins increasingly cannot provide domestic older population care, and human investment in training caregivers is an urgent task of Chinese policymaking. Therefore, how Chinese policymaking encourages upskilling or reskilling relevant caregivers is examined in this narrative essay.
Healthcare Providers’ Perceptions about the Role of Spiritual Care and Chaplaincy Services in Substance Use Outpatient Treatment
Authors: Earl Brian S. W. , Klee Anne , Edens Ellen L. , Cooke James D. , Heikkila Holly , Grau Lauretta E.
Published: August 2022

Addressing patients’ religion and spirituality (R/S) needs has been associated with positive health outcomes. However, despite receiving extensive training in spiritual assessment and care, chaplaincy services are primarily confined to inpatient settings, with few studies occurring in outpatient settings. The study sought to understand mental health providers’ views about what shaped provider and patient motivation to engage in R/S discussions and seek referrals to chaplaincy services. We conducted five one-hour focus group sessions with a total of 38 staff members and thematically analyzed the resulting session and field notes. We identified four themes concerning provider knowledge and attitudes about R/S and chaplaincy services: Staff Information Needs, Staff Motivation to Discuss R/S and Refer, Patient Motivation to Use Chaplaincy Services, and Chaplain Accessibility. The study findings suggest that providers in outpatient substance use treatment clinics in the Veterans Health Administration are receptive to learning about R/S care and the possibility of expanding chaplaincy services. However, staff have misconceptions about the roles and responsibilities of chaplains. Attitudes about and experiences with R/S discussions varied. Trust and confidence in the benefits of chaplaincy services may be improved among both providers and patients by increasing chaplains’ accessibility and visibility within these outpatient settings.
Workplace Physical Activity Barriers and Facilitators: A Qualitative Study Based on Employees Physical Activity Levels
Authors: Safi Ayazullah , Cole Matthew , Kelly Adam L. , Zariwala Mohammed Gulrez , Walker Natalie C.
Published: August 2022

Introduction: Lack of time, management support, insufficient facilitates, workload balance, and culture are often reported as common barriers to physical activity (PA) participation in the workplace. In comparison, identifying facilitators of PA in the workplace are scarce. A ‘one-size-fits-all’ approach to overcoming the barriers may also be unsuccessful within university settings where multidisciplinary workforce exists due to the heterogeneity nature of job roles. Thus, the aim of this study was to understand the perceived barriers and facilitators of PA of university employees who were classified as active or inactive based on their job roles. Methods: Forty-one employees (female = 17; male = 24) participated in focus groups to discuss their perceived barriers and facilitators to PA in the workplace. Participants were categorised based on their PA levels as active and inactive prior analysing the semi-structured focus groups data via using thematic analysis. Results and Discussion: The results showed that a lack of time was reported by 80% of the participants as a barrier to PA, including 63% inactive and 17% of the active participants. This included 27% administrators’ staff, 23% academics, 19% senior management, and 11% professional service staff. Over 75% participants reported a lack of management support as one of the perceived barriers to their PA engagement in the workplace. Approximately 58% also reported workplace culture as a barrier to PA participation. Open access to a gym on campus was perceived to be the main facilitator to engaging in PA in the future. Similarly, increased management support for engaging in PA and having flexibility during working days were perceived as facilitators for PA engagement and a way to reduced sedentary behaviour in the workplace. Conclusions: These findings contribute to the limited literature in terms of evaluating obstacles and facilitators of university employees to encourage engagement with PA in the workplace. These findings can be applied to form PA, health, and wellbeing-related interventions specifically targeting these identified barriers that are experienced in the workplace and thereby potentially reducing absenteeism and increasing productivity.
Psychological Functioning, Defense Strategies and the Contribution of Perceived Family Collaboration in Adolescents Who Experienced Multiple Motor Vehicle Crashes: A Descriptive Study
Authors: Di Vito Paola , Cerniglia Luca , Cimino Silvia
Published: August 2022

Adolescents frequently experience motor vehicle collisions (MVCs). Research has mostly underlined the psychological outcomes, rather than adolescents’ general emotional–behavioral functioning and the role played by family. This study aims to explore the emotional–behavioral functioning, measured with the Youth Self Report (YSR), difficulties to identify and describe emotions, measured with the Toronto Alexithymia Scale (TAS-20), the use of defense strategies, measured with the Response Evaluation Measure for Youth (REM-71), and perceived family collaboration, measured with the Perceived Collective Family scale, in adolescents that have experienced more than three MVCs in a year. N = 150 adolescents who visited an emergency department for MVCs were assessed through self-report questionnaires. Adolescents showed difficulties to identify and describe their emotions and a massive use of defense strategies. Moreover, lower perceived family collaboration predicted adolescents’ alexithymic traits and the massive use of maladaptive defense strategies. These results may be useful in assessing and creating prevention programs for risky driving behaviors in adolescence.
Identification of Urban Functional Zones Based on the Spatial Specificity of Online Car-Hailing Traffic Cycle
Authors: Deng Zhicheng , You Xiangting , Shi Zhaoyang , Gao Hong , Hu Xu , Yu Zhaoyuan , Yuan Linwang
Published: August 2022

The study of urban functional zoning is not only important for analyzing urban spatial structure but also for optimizing urban management and promoting scientific urban planning. Different areas undertaking different urban functions correspond to different traffic patterns and specific cycles. Here, a method named Urban Functional Zoning based on the Spatial Specificity (UFZ-SS) is proposed. The core of this method is to obtain urban spatial zoning through the specific cycles of traffic flows. First, UFZ-SS uses the Ensemble Empirical Modal Decomposition (EEMD) method to extract the specific periodic signal characteristics of traffic flows. Second, UFZ-SS calculates the contribution of online car-hailing traffic of different cycles in each zone. Then, the Gaussian Mixture Model (GMM) is utilized to classify all spatial zones into different spatial partitions based on the contribution of each periodic signal. Finally, this study validates UFZ-SS with the online car-hailing traffic volume in northeast Chengdu, China. The results show that the periodic characteristics of traffic can be effectively extracted and analyzed by the EEMD method, and highly distinct and accurate urban spatial partitioning results can be derived by spatial clustering based on the measures of specific cycles. Moreover, with the assistance of Point of Interest (POI) data, we verify the functional zones and structural patterns, which further demonstrates the validity and rationality of urban functional zones identified by UFZ-SS. This study provides a new potential perspective for the identification of urban functional zones, which may lead to a better understanding of the urban spatial structure and even urban planning.
Immunotherapy for the Treatment of Squamous Cell Carcinoma: Potential Benefits and Challenges
Authors: Ansary Tuba M. , Hossain MD Razib , Komine Mayumi , Ohtsuki Mamitaro
Published: August 2022

Melanoma and nonmelanoma skin cancers (NMSCs) are recognized as among the most common neoplasms, mostly in white people, with an increasing incidence rate. Among the NMSCs, squamous cell carcinoma (SCC) is the most prevalent malignancy known to affect people with a fair complexion who are exposed to extreme ultraviolet radiation (UVR), have a hereditary predisposition, or are immunosuppressed. There are several extrinsic and intrinsic determinants that contribute to the pathophysiology of the SCC. The therapeutic modalities depend on the SCC stages, from actinic keratosis to late-stage multiple metastases. Standard treatments include surgical excision, radiotherapy, and chemotherapy. As SCC represents a favorable tumor microenvironment with high tumor mutational burden, infiltration of immune cells, and expression of immune checkpoints, the SCC tumors are highly responsive to immunotherapies. Until now, there are three checkpoint inhibitors, cemiplimab, pembrolizumab, and nivolumab, that are approved for the treatment of advanced, recurrent, or metastatic SCC patients in the United States. Immunotherapy possesses significant therapeutic benefits for patients with metastatic or locally advanced tumors not eligible for surgery or radiotherapy to avoid the potential toxicity caused by the chemotherapies. Despite the high tolerability and efficiency, the existence of some challenges has been revealed such as, resistance to immunotherapy, less availability of the biomarkers, and difficulty in appropriate patient selection. This review aims to accumulate evidence regarding the genetic alterations related to SCC, the factors that contribute to the potential benefits of immunotherapy, and the challenges to follow this treatment regime.
Quantitative Characterisation of Low Abundant Yeast Mitochondrial Proteins Reveals Compensation for Haplo-Insufficiency in Different Environments
Authors: Manousaki Alkisti , Bagnall James , Spiller David , Balarezo-Cisneros Laura Natalia , White Michael , Delneri Daniela
Published: August 2022

The quantification of low abundant membrane-binding proteins such as transcriptional factors and chaperones has proven difficult, even with the most sophisticated analytical technologies. Here, we exploit and optimise the non-invasive Fluorescence Correlation Spectroscopy (FCS) for the quantitation of low abundance proteins, and as proof of principle, we choose two interacting proteins involved in the fission of mitochondria in yeast, Fis1p and Mdv1p. In Saccharomyces cerevisiae, the recruitment of Fis1p and Mdv1p to mitochondria is essential for the scission of the organelles and the retention of functional mitochondrial structures in the cell. We use FCS in single GFP-labelled live yeast cells to quantify the protein abundance in homozygote and heterozygote cells and to investigate the impact of the environments on protein copy number, bound/unbound protein state and mobility kinetics. Both proteins were observed to localise predominantly at mitochondrial structures, with the Mdv1p bound state increasing significantly in a strictly respiratory environment. Moreover, a compensatory mechanism that controls Fis1p abundance upon deletion of one allele was observed in Fis1p but not in Mdv1p, suggesting differential regulation of Fis1p and Mdv1p protein expression.
The Role of Histology-Agnostic Drugs in the Treatment of Metastatic Castration-Resistant Prostate Cancer
Authors: Iannantuono Giovanni Maria , Torino Francesco , Rosenfeld Roberto , Guerriero Simona , Carlucci Manuela , Sganga Stefano , Capotondi Barbara , Riondino Silvia , Roselli Mario
Published: August 2022

Precision medicine has opened up a new era in the development of anti-cancer agents that is focused on identifying biomarkers predictive of treatment response regardless of tumor histology. Since 2017, the Food and Drug Administration has approved six drugs with histology-agnostic indications: pembrolizumab (both for tumors with the mismatch-repair deficiency (dMMR)/high microsatellite instability (MSI-H) phenotype and for those with the high tumor mutational burden (TMB-H) phenotype), dostarlimab (for dMMR tumors), larotrectinib and entrectinib (for tumors harboring neurotrophic tyrosine receptor kinase (NTRK) fusions), and the combination of dabrafenib plus trametinib (for BRAF V600E-mutated tumors). The genomic alterations targeted by these antineoplastic agents are rare in metastatic castration-resistant prostate cancer (mCRPC). Furthermore, only a small number of mCRPC patients were enrolled in the clinical trials that led to the approval of the above-mentioned drugs. Therefore, we critically reviewed the literature on the efficacy of histology-agnostic drugs in mCRPC patients. Although the available evidence derives from retrospective studies and case reports, our results confirmed the efficacy of pembrolizumab in dMMR/MSI-H mCRPC. In contrast, few data are available for dostarlimab, larotrectinib, entrectinib, and dabrafenib-trametinib in this subset of patients. Large, multi-institutional registries aimed at collecting real-world data are needed to better comprehend the role of tissue-agnostic drugs in mCRPC patients.
CCL18 Expression Is Higher in a Glioblastoma Multiforme Tumor than in the Peritumoral Area and Causes the Migration of Tumor Cells Sensitized by Hypoxia
Authors: Grochans Szymon , Korbecki Jan , Simińska Donata , Żwierełło Wojciech , Rzeszotek Sylwia , Kolasa Agnieszka , Kojder Klaudyna , Tarnowski Maciej , Chlubek Dariusz , Baranowska-Bosiacka Irena
Published: August 2022

Glioblastoma multiforme (GBM) is a brain tumor with a very poor prognosis. For this reason, researchers worldwide study the impact of the tumor microenvironment in GBM, such as the effect of chemokines. In the present study, we focus on the role of the chemokine CCL18 and its receptors in the GBM tumor. We measured the expression of CCL18, CCR8 and PITPNM3 in the GMB tumor from patients (16 men and 12 women) using quantitative real-time polymerase chain reaction. To investigate the effect of CCL18 on the proliferation and migration of GBM cells, experiments were performed using U-87 MG cells. The results showed that CCL18 expression was higher in the GBM tumor than in the peritumoral area. The women had a decreased expression of PITPNM3 receptor in the GBM tumor, while in the men a lower expression of CCR8 was observed. The hypoxia-mimetic agent, cobalt chloride (CoCl2), increased the expression of CCL18 and PITPNM3 and thereby sensitized U-87 MG cells to CCL18, which did not affect the proliferation of U-87 MG cells but increased the migration of the test cells. The results indicate that GBM cells migrate from hypoxic areas, which may be important in understanding the mechanisms of tumorigenesis.
A Study on Superior Mesoporous Activated Carbons for Ultra Power Density Supercapacitor from Biomass Precursors
Authors: Bang Joon-Hyuk , Lee Byeong-Hoon , Choi Young-Chul , Lee Hye-Min , Kim Byung-Joo
Published: August 2022

A kenaf-derived activated carbon (KAC) for a high-power density supercapacitor was developed in this study through phosphoric acid activation. The N2/77K isothermal adsorption–desorption curve was used to estimate the textural properties of KAC based on BET and BJH and the pore size distribution based on NLDFT. The electrochemical properties of KAC were analyzed by using the coin-type cell applying 1 M SPBBF4/PC electrolyte, and the specific surface area and total pore volume were 1490–1942 m2/g and 1.18–3.18 cm3/g, respectively. The pore characteristics of KAC varied according to the activation temperature, and most KAC showed a mesoporous structure. As the activation temperature increased, the mesopore volume increased up to 700 °C, then decreased. The mesoporous structure of KAC resulted in a substantial decrease in the Warburg impedance as the ion diffusion resistance decreased. Hence, the specific capacitance of KAC decreased from 82.9 F/g to 59.48 F/g as the charge–discharge rate increased from 1 mA/g to 10 mA/g, with the rate of reduction at approximately 30%. The rate of reduction of KAC’s specific capacitance was 50% lower compared with commercial activated carbon; hence, KAC is a more suitable electrode-active material for high power density supercapacitors.
Enhancer Regulation of Dopaminergic Neurochemical Transmission in the Striatum
Authors: Harsing Laszlo , Knoll Joseph , Miklya Ildiko
Published: August 2022

The trace amine-associated receptor 1 (TAAR1) is a Gs protein-coupled, intracellularly located metabotropic receptor. Trace and classic amines, amphetamines, act as agonists on TAAR1; they activate downstream signal transduction influencing neurotransmitter release via intracellular phosphorylation. Our aim was to check the effect of the catecholaminergic activity enhancer compound ((−)BPAP, (R)-(−)-1-(benzofuran-2-yl)-2-propylaminopentane) on neurotransmitter release via the TAAR1 signaling. Rat striatal slices were prepared and the resting and electrical stimulation-evoked [3H]dopamine release was measured. The releaser (±)methamphetamine evoked non-vesicular [3H]dopamine release in a TAAR1-dependent manner, whereas (−)BPAP potentiated [3H]dopamine release with vesicular origin via TAAR1 mediation. (−)BPAP did not induce non-vesicular [3H]dopamine release. N-Ethylmaleimide, which inhibits SNARE core complex disassembly, potentiated the stimulatory effect of (−)BPAP on vesicular [3H]dopamine release. Subsequent analyses indicated that the dopamine-release stimulatory effect of (−)BPAP was due to an increase in PKC-mediated phosphorylation. We have hypothesized that there are two binding sites present on TAAR1, one for the releaser and one for the enhancer compounds, and they activate different PKC-mediated phosphorylation leading to the evoking of non-vesicular and vesicular dopamine release. (−)BPAP also increased VMAT2 operation enforcing vesicular [3H]dopamine accumulation and release. Vesicular dopamine release promoted by TAAR1 evokes activation of D2 dopamine autoreceptor-mediated presynaptic feedback inhibition. In conclusion, TAAR1 possesses a triggering role in both non-vesicular and vesicular dopamine release, and the mechanism of action of (−)BPAP is linked to the activation of TAAR1 and the signal transduction attached.
Correction: Bok et al. Super-Toughened Fumed-Silica-Reinforced Thiol-Epoxy Composites Containing Epoxide-Terminated Polydimethylsiloxanes. Int. J. Mol. Sci. 2021, 22, 8097
Authors: Bok Goseong , Lim Gayoung , Kwak Mingi , Kim Youngmin
Published: August 2022

The authors wish to make the following corrections to the original publication [...]
Time-Dependent DNA Origami Denaturation by Guanidinium Chloride, Guanidinium Sulfate, and Guanidinium Thiocyanate
Authors: Hanke Marcel , Hansen Niklas , Tomm Emilia , Grundmeier Guido , Keller Adrian
Published: August 2022

Guanidinium (Gdm) undergoes interactions with both hydrophilic and hydrophobic groups and, thus, is a highly potent denaturant of biomolecular structure. However, our molecular understanding of the interaction of Gdm with proteins and DNA is still rather limited. Here, we investigated the denaturation of DNA origami nanostructures by three Gdm salts, i.e., guanidinium chloride (GdmCl), guanidinium sulfate (Gdm2SO4), and guanidinium thiocyanate (GdmSCN), at different temperatures and in dependence of incubation time. Using DNA origami nanostructures as sensors that translate small molecular transitions into nanostructural changes, the denaturing effects of the Gdm salts were directly visualized by atomic force microscopy. GdmSCN was the most potent DNA denaturant, which caused complete DNA origami denaturation at 50 °C already at a concentration of 2 M. Under such harsh conditions, denaturation occurred within the first 15 min of Gdm exposure, whereas much slower kinetics were observed for the more weakly denaturing salt Gdm2SO4 at 25 °C. Lastly, we observed a novel non-monotonous temperature dependence of DNA origami denaturation in Gdm2SO4 with the fraction of intact nanostructures having an intermediate minimum at about 40 °C. Our results, thus, provide further insights into the highly complex Gdm–DNA interaction and underscore the importance of the counteranion species.
Thiosemicarbazones Can Act Synergistically with Anthracyclines to Downregulate CHEK1 Expression and Induce DNA Damage in Cell Lines Derived from Pediatric Solid Tumors
Authors: Paukovcekova Silvia , Krchniakova Maria , Chlapek Petr , Neradil Jakub , Skoda Jan , Veselska Renata
Published: August 2022

Anticancer therapy by anthracyclines often leads to the development of multidrug resistance (MDR), with subsequent treatment failure. Thiosemicarbazones have been previously suggested as suitable anthracycline partners due to their ability to overcome drug resistance through dual Pgp-dependent cytotoxicity-inducing effects. Here, we focused on combining anthracyclines (doxorubicin, daunorubicin, and mitoxantrone) and two thiosemicarbazones (DpC and Dp44mT) for treating cell types derived from the most frequent pediatric solid tumors. Our results showed synergistic effects for all combinations of treatments in all tested cell types. Nevertheless, further experiments revealed that this synergism was independent of Pgp expression but rather resulted from impaired DNA repair control leading to cell death via mitotic catastrophe. The downregulation of checkpoint kinase 1 (CHEK1) expression by thiosemicarbazones and the ability of both types of agents to induce double-strand breaks in DNA may explain the Pgp-independent synergism between anthracyclines and thiosemicarbazones. Moreover, the concomitant application of these agents was found to be the most efficient approach, achieving the strongest synergistic effect with lower concentrations of these drugs. Overall, our study identified a new mechanism that offers an avenue for combining thiosemicarbazones with anthracyclines to treat tumors regardless the Pgp status.
Metformin Preserves VE–Cadherin in Choroid Plexus and Attenuates Hydrocephalus via VEGF/VEGFR2/p-Src in an Intraventricular Hemorrhage Rat Model
Authors: Shen Dan , Ye Xianghua , Li Jiawen , Hao Xiaodi , Jin Luhang , Jin Yujia , Tong Lusha , Gao Feng
Published: August 2022

Hydrocephalus induced by intraventricular hemorrhage (IVH) is associated with unfavorable prognosis. The increased permeability of choroid plexus and breakdown of the blood–brain barrier (BBB) was reported as a prominent mechanism of IVH-induced hydrocephalus, and vascular endothelial–cadherin (VE–cadherin) was demonstrated to be relevant. Metformin was reported to protect endothelial junction and preserve permeability widely; however, its role in hydrocephalus remains unclear. In this study, the decreased expression of VE–cadherin in the choroid plexus, accompanied with ventricle dilation, was investigated in an IVH rat model induced by intraventricular injection of autologous blood. Metformin treatment ameliorated hydrocephalus and upregulated VE–cadherin expression in choroid plexus meanwhile. We then observed that the internalization of VE–cadherin caused by the activation of vascular endothelial growth factor (VEGF) signaling after IVH was related to the occurrence of hydrocephalus, whereas it can be reversed by metformin treatment. Restraining VEGF signaling by antagonizing VEGFR2 or inhibiting Src phosphorylation increased the expression of VE–cadherin and decreased the severity of hydrocephalus after IVH. Our study demonstrated that the internalization of VE–cadherin via the activation of VEGF signaling may contribute to IVH-induced hydrocephalus, and metformin may be a potential protector via suppressing this pathway.
Functional Characterization of Tomato ShROP7 in Regulating Resistance against Oidium neolycopersici
Authors: Meng Yanan , Zhang Ancheng , Ma Qing , Xing Lianxi
Published: August 2022

ROPs (Rho-like GTPases from plants) are a unique family of small GTP-binding proteins in plants and play vital roles in numerous cellular processes, including growth and development, abiotic stress signaling, and plant defense. In the case of the latter, the role of ROPs as response regulators to obligate parasitism remains largely enigmatic. Herein, we isolated and identified ShROP7 and show that it plays a critical role in plant immune response to pathogen infection. Real-time quantitative PCR analysis revealed that the expression of ShROP7 was significantly increased during incompatible interactions. To establish its requirement for resistance, we demonstrate that virus-induced gene silencing (VIGS) of ShROP7 resulted in increased susceptibility of tomato to Oidium neolycopersici (On) Lanzhou strain (On-Lz). Downstream resistance signaling through H2O2 and the induction of the hypersensitive response (HR) in ShROP7-silenced plants were significantly reduced after inoculating with On-Lz. Taken together, with the identification of ShROP7-interacting candidates, including ShSOBIR1, we demonstrate that ShROP7 plays a positive regulatory role in tomato powdery mildew resistance.
Investigation of Gut Bacterial Communities of Asian Citrus Psyllid (Diaphorina citri) Reared on Different Host Plants
Authors: Meng Lixue , Xia Changxiu , Jin Zhixiong , Zhang Hongyu
Published: August 2022

Diaphorina citri Kuwayama (Hemiptera: Liviidae) can cause severe damage to citrus plants, as it transmits Candidatus Liberibacter spp., a causative agent of Huanglongbing disease. Symbiotic bacteria play vital roles in the ecology and biology of herbivore hosts, thereby affecting host growth and adaptation. In our research, the effects of Rutaceous plants (i.e., Citrus reticulata cv. Shatangju, Citrus poonensis cv. Ponkan, Murraya paniculata (orange jasmine), Citrus limon (lemon), and Citrus sinensis (navel orange)) on the gut microbiota (GM) and microbial diversity of D. citri adults were investigated by 16S rRNA high-throughput sequencing. It was found that Proteobacteria dominated the GM communities. The gut microbe diversity was the highest in the ponkan-feeding population, and the lowest in the Shatangju-feeding population. The NMDS analysis revealed that there were obvious differences in the GM communities among the different hosts. PICRUSt function prediction indicated significant differences in host function, and those pathways were crucial for maintaining population reproduction, growth, development, and adaptation to environmental stress in D. citri. Our study sheds new light on the interactions between symbionts, herbivores, and host plants and expands our knowledge on host adaptation related to GM in D. citri.
Upgrade of the HIVIPP Deposition Apparatus for Nuclear Physics Thin Targets Manufacturing
Authors: Cisternino Sara , Skliarova Hanna , Antonini Piergiorgio , Esposito Juan , Mou Liliana , Pranovi Lorenzo , Pupillo Gaia , Sciacca Gabriele
Published: August 2022

The High Energy Vibrational Powder Plating (HIVIPP) technique allows for the preparation of targets starting from refractory metal powders with negligible material losses during the process, thus preserving the expensive isotope-enriched materials. An upgraded HIVIPP apparatus was developed at the Legnaro National Laboratory of the National Institute of Nuclear Physics (INFN-LNL), and it is reported in this work. Particular attention was paid to the design of the sample holder, the automation of the power supply, and the control of the process, all with the aim of obtaining a versatile and reliable apparatus. Several tests have been carried out and the related results are reported proving the flexibility of the apparatus and the process reproducibility. The main result is a ‘ready to use’ technology at INFN-LNL for the preparation of isotopically enriched refractory metal targets that cannot be manufactured using standard techniques.
Prognostic Value and Clinical Significance of FGFR Genomic Alterations (GAs) in Metastatic Urothelial Cancer Patients
Authors: Sevillano Fernández Elena , Madurga de Lacalle Rodrigo , Rodriguez Moreno Juan Francisco , Barquín García Arantzazu , Yagüe Fernández Mónica , Navarro Alcaraz Paloma , Barba Llacer María , Quiralte Pulido Miguel , García-Donás Jiménez Jesús
Published: August 2022

Fibroblast growth factor receptor (FGFR) genomic alterations (GAs) represent an actionable target, key to the pathogenesis of some urothelial cancers (UCs). Though FGFR GAs are common in noninvasive UC, little is known about their role in the metastatic(m) setting and response to therapy. This study aimed to assess the impact of FGFR alterations on sensitivity to systemic treatments and survival and to validate Bajorin’s and Bellmunt’s prognostic scores in mUC patients according to their FGFR status. We retrospectively analyzed data from 98 patients with tumor-sequenced UC who received treatment between January 2010 and December 2020. Up to 77 developed metastatic disease and were deemed the study population. Twenty-six showed FGFR GAs. A trend toward a better response to cisplatin and checkpoint inhibitors was suggested favoring FGFR GA tumors. FGFR GA patients who received an FGFR inhibitor as first-line had poorer responses compared with other options (20% vs. 68.4%, p = 0.0065). Median PFS was 6 vs. 5 months in the FGFR GA vs. FGFR WT cohort (p = 0.71). Median OS was significantly worse in the FGFR GA vs. FGFR WT cohort (16.2 vs. 31.9 months, p = 0.045). Multivariate analyses deemed FGFR GAs as a factor independently associated with the outcome (HR 2.59 (95% CI 1.21–5.55)). Bajorin’s model correctly predicted clinical outcomes in the whole study population but not in FGFR GA cases. FGFR GAs are a relevant biomarker in mUC that could condition the response to systemic therapy. New prognostic models, including this molecular determination, should be designed and validated.
Association between the Use of Antibiotics and the Development of Acute Renal Injury in Patients Hospitalized for COVID-19 in a Hospital in the Peruvian Amazon
Authors: Romaní Luccio , León-Figueroa Darwin A. , Rafael-Navarro David , Barboza Joshuan J. , Rodriguez-Morales Alfonso J.
Published: August 2022

Introduction: A significant antibiotic prescribing pattern associated with the COVID-19 pandemic has been described. Multiple protocols included empirical antimicrobials, leading to a substantial increase in antimicrobial consumption in medical care. A higher mortality rate is described among patients diagnosed with COVID-19 who received antibiotics. Objectives: To determine the association between the use of antibiotics and the development of acute renal injury in patients infected with SARS-CoV-2 in patients treated at the Hospital II EsSalud de Ucayali, 2021. Methods: A cross-sectional-analytical study was conducted, evaluating the medical records of patients admitted to the intensive care unit between July 2020 and July 2021. For the statistical analysis, measures of central tendency and dispersion, statistical hypothesis contrast tests were used in relation to acute kidney injury (AKI), antibiotic use and associated factors, derived from linear regression models. Results: The factors that were positively associated with the development of AKI were sepsis (aPR: 2.86; 95% CI: 1.26–6.43), shock (aPR:2.49; 95% CI: 1.28–4.86), mechanical ventilation (aPR:9.11; 95% CI: 1.23–67.57), and use of vancomycin (aPR: 3.15; 95% CI: 1.19–8.27). Conclusions: In the Peruvian Amazon, there is a high consumption and inadequate prescription of antibiotics. The drugs most commonly used for the treatment of COVID-19 were: aminoglycosides, vancomycin, ivermectin, azithromycin, tocilizumab, and corticosteroids. The development of AKI among hospitalized patients was found to be related to vancomycin administration. In addition, an association was found with the use of mechanical ventilation, a high body mass index, and the presence of complications such as sepsis or shock. Therefore, inappropriate antibiotic use for COVID-19 has been associated with multiple negative outcomes and consequences.
Targeted Data Augmentation and Hierarchical Classification with Deep Learning for Fish Species Identification in Underwater Images
Authors: Ben Tamou Abdelouahid , Benzinou Abdesslam , Nasreddine Kamal
Published: August 2022

In this paper, we address fish species identification in underwater video for marine monitoring applications such as the study of marine biodiversity. Video is the least disruptive monitoring method for fish but requires efficient techniques of image processing and analysis to overcome challenging underwater environments. We propose two Deep Convolutional Neural Network (CNN) approaches for fish species classification in unconstrained underwater environment. In the first approach, we use a traditional transfer learning framework and we investigate a new technique based on training/validation loss curves for targeted data augmentation. In the second approach, we propose a hierarchical CNN classification to classify fish first into family levels and then into species categories. To demonstrate the effectiveness of the proposed approaches, experiments are carried out on two benchmark datasets for automatic fish identification in unconstrained underwater environment. The proposed approaches yield accuracies of 99.86% and 81.53% on the Fish Recognition Ground-Truth dataset and LifeClef 2015 Fish dataset, respectively.
Emerging Trends in the Use of Topical Antifungal-Corticosteroid Combinations
Authors: Mijaljica Dalibor , Spada Fabrizio , Harrison Ian P.
Published: August 2022

A broad range of topical antifungal formulations containing miconazole or terbinafine as actives are commonly used as efficacious choices for combating fungal skin infections. Their many benefits, owing to their specific mechanism of action, include their ability to target the site of infection, enhance treatment efficacy and reduce the risk of systemic side effects. Their proven efficacy, and positioning in the treatment of fungal skin infections, is enhanced by high patient compliance, especially when appropriate vehicles such as creams, ointments and gels are used. However, inflammation as a result of fungal infection can often impede treatment, especially when combined with pruritus (itch), an unpleasant sensation that elicits an urge to scratch. The scratching that occurs in response to pruritus frequently accelerates skin damage, ultimately aggravating and spreading the fungal infection. To help overcome this issue, a topical antifungal-corticosteroid combination consisting of miconazole or terbinafine and corticosteroids of varying potencies should be used. Due to their inherent benefits, these topical antifungal-corticosteroid combinations can concomitantly and competently attenuate inflammation, relieve pruritus and treat fungal infection.
Comparative Efficacy of Interventions for the Management of Oral Submucous Fibrosis: A Systematic Review and Network Meta-Analysis
Authors: Gopinath Divya , Hui Lai Mong , Veettil Sajesh K. , Balakrishnan Nair Athira , Maharajan Mari Kannan
Published: August 2022

Introduction: Oral submucous fibrosis (OSMF) is a chronic premalignant condition and is characterized by fibroblastic change of lamina propria and stiffness of oral mucosa. Though there are several treatment options available, the best agent is not yet identified. This study assessed the comparative efficacy and safety of medical interventions in the management of OSMF. Methods: A systematic review was performed to identify randomized controlled trials (RCTs) that compared the efficacy of interventions for OSMF with each other, or placebo. A network meta-analysis was performed, and the interventions were ranked according to their efficacy based on the surface under the cumulative ranking. (PROSPERO Registration no: CRD42021255094). Results: Thirty-two RCTs comprising 2063 patients were eligible for quantitative analysis. In terms of therapeutic efficacy in the improvement of mouth opening Oxitard, a herbal formulation was ranked as the most efficacious agent, [MD, 10.29 (95%CI 6.34–14.25)] followed by combination therapy of Lycopene with corticosteroids and hyaluronidase [MD, 7.07 (95%CI 1.82–12.31)]. For improvement of burning sensation aloe vera was ranked first [MD, 6.14 (95%CI 4.58–7.70)] followed by corticosteroids with antioxidants [MD, 6.13 (95%CI 4.12–8.14)] and corticosteroids in combination with hyaluronidase with antioxidants [MD, 5.95 (95%CI 3.79–8.11)]. In terms of safety, most of the drugs were reported to cause mild adverse effects only. Significant inconsistencies could be identified in the analysis for both the outcomes assessed and were further explored. Conclusions: Our study highlighted the potential efficacy of several agents over placebo in the improvement of mouth opening and burning sensation in OSMF patients. However, the RCTs lacked methodological soundness. Well-designed studies with a larger number of participants with a rigorous randomization process and stringent methodology are recommended to strengthen the results obtained, which may help to construct a clinical guideline for OSMF management.
Zoo Closure Does Not Affect Behavior and Activity Patterns of Palawan Binturong (Arctictis binturong whitei)
Authors: Finch Katherine , Leary Megan , Holmes Lisa , Williams Leah J.
Published: August 2022

Exploring the interaction between humans and animals has become increasingly important in the evaluation of well-being for species housed in zoos and aquaria. The COVID-19 pandemic saw the global closure of zoos and aquaria to visitors. Chester Zoo, U.K., was no exception, with the charity shutting its doors for the longest period in its 90-year history. Whilst access to site was strictly limited to essential animal care staff, recent investment in networked infrared CCTV camera systems allowed some species to be monitored remotely during this extraordinary period of zoo closure. Here, we used this equipment to investigate whether zoo closure influenced activity patterns and behavior of two adult Palawan binturong, Arctictis binturong whitei. The cameras facilitated behavioral monitoring over 24 h enabling the collection of a full activity budget, which revealed a natural crepuscular activity pattern. Overall, visitor presence was found to have a neutral effect on this species, with no significant difference observed in time spent engaging in den use, vigilance or travel behaviors during zoo open and zoo closed conditions. A neutral visitor effect was found when evaluating behavior over a 24 h period and during hours which the zoo would normally be open to visitors (10:00–16:30). This research presents new information on this elusive and understudied species in captivity, and promotes investment in monitoring equipment which enables more comprehensive behavioral sampling than traditional visitor-effect methods.
Measuring Gross Ecosystem Product (GEP) in Guangxi, China, from 2005 to 2020
Authors: Wang Luying , Su Kai , Jiang Xuebing , Zhou Xiangbei , Yu Zhu , Chen Zhongchao , Wei Changwen , Zhang Yiming , Liao Zhihong
Published: August 2022

The economic and social development evaluation system with the Gross Domestic Product (GDP) as the leading indicator is no longer applicable to the current social progress in China. It is essential to carry out an assessment of the Gross Ecosystem Product (GEP) to integrate ecological benefits into the economic and social evaluation system and promote sustainable socio-economic development. This study took Guangxi, an important province in South China, as the study area. We used four periods of land use and land cover data (LULC), meteorological data, soil data and yearbook statistics to construct a GEP assessment framework based on geographic information system (GIS) and remote sensing (RS) technologies. We accounted for the provisioning services, regulating services, and tourism services provided by Guangxi in 2005, 2010, 2015, and 2020 and analyzed the region’s and municipalities’ spatial–temporal pattern characteristics and trends of change in GEP. In addition, this study also discusses the relationship between GEP and GDP. The results showed that many important products and services provided by natural ecosystems in Guangxi had enormous economic benefits. GEP had increased from CNY 15,657.37 billion in 2005 to CNY 36,677.04 billion in 2020, and the distribution of GEP showed obvious spatial heterogeneity. The value of ecosystem regulation services was about 65–89% of GEP, which is the main component of GEP. From 2005 to 2020, natural ecosystem protection and socio-economic development have achieved coordinated development in Guangxi. GEP and GDP showed upward trends in general. Although Guangxi is relatively backward in terms of economic development, the scientific quantification of the unrealized value of the services provided by the ecosystem through GEP accounting makes it possible to transform ecological advantages into economic advantages. It could help the local government and people to re-recognize the value of ecological resources and realize the beautiful vision of lucid waters and lush mountains as invaluable assets.
Reuse of Historic Buildings in the Medieval City of Rhodes to Comply with the Needs of Sustainable Urban Development
Authors: Giannakopoulos Dimitris , Karekou Zografia , Menegaki Elli , Tsilimantou Elisavet , Ioannidis Charalabos , Maistrou Eleni , Giannikouris Antonios , Moropoulou Antonia
Published: August 2022

This paper illustrates a study for the reuse of selected historic buildings in the Medieval City of Rhodes in Greece. This study contributes to the understanding and interpretation of the chronological sequence of major intervention programs for the preservation of the Medieval City. The main idea of the project is the spatial distribution of compatible and various uses to reveal the unique character of the Medieval City. Spatial planning is proposed within the preserved urban zones in order to optimize and harmonize the selected uses according to the needs of sustainable urban development. Criteria to assess the compatible uses that ensure the features and architectural characteristics of the preselected historic buildings, located within the city fabric, were established and validated. The developed methodology that is presented herein and is an indispensable part of a pilot project may be applicable to other cases concerning historic cities. Finally, this paper aims to present a pilot program which promotes the reuse of historic buildings as a part of an integrated preservation plan. Inter-disciplinarity has set the basis for effective policies to guide and control the proposed pilot program, with ultimate objective to ensure sustainable preservation of the Medieval City of Rhodes.
Demonstrative Systems Are Not Affected by Contact: Evidence from Heritage Southern Italo-Romance
Authors: Terenghi Silvia
Published: August 2022

Deictic information is present in every language; yet, there are significant differences as to how exactly such information is encoded, yielding different indexical systems across languages. The availability of cross-linguistic variation in indexical systems provides a window into the role of contact in shaping grammars: this work contributes to the discussion by investigating whether contact plays any role in determining the grammar of indexicality in heritage varieties. This study has a two-fold aim. Empirically, it investigates ternary demonstrative systems in heritage southern Italo-Romance varieties: on the basis of comprehension and production data, these systems are shown to be in the process of undergoing change. Theoretically, it underscores the insights that the combined microcontact and diachronic perspective provides for the understanding of variation and change in heritage languages: while, at face value, the elicited heritage data seem to indicate that demonstratives are affected by contact, pairwise comparisons across heritage varieties and diachronic observations lead to rejecting a plain contact-induced explanation and to conclude, instead, that deictic elements are largely unaffected by contact and that their change in heritage varieties is, rather, endogenous.
Gamma Knife Radiosurgery for Indirect Dural Carotid–Cavernous Fistula: Long-Term Ophthalmological Outcome
Authors: Shen Chiung-Chyi , Tsuei Yuang-Seng , Yang Meng-Yin , You Weir-Chiang , Sun Ming-His , Sheu Meei-Ling , Pan Liang-Yi , Sheehan Jason , Pan Hung-Chuan
Published: August 2022

Objective: The leading treatment option for dural carotid–cavernous sinus fistula is an endovascular approach with immediate improvement. Alternatively, radiosurgery is a slow response for obliterating the fistula and poses a radiation risk to the optic apparatus and the associated cranial nerves and blood vessels. In this study, we retrieved cases from a prospective database to assess the ophthalmological outcomes and complications in treating dural carotid cavernous sinus fistula with gamma knife radiosurgery (GKRS). Material and Methods: We retrieved a total of 65 cases of carotid cavernous sinus fistula treated with GKRS with margin dose of 18–20 Gy from 2003 to 2018 and reviewed the ophthalmological records required for our assessment. Results: The mean target volume was 2 ± 1.43 cc. The onset of symptom alleviated after GKRS was 3.71 ± 7.68 months. There were two cases with residual chemosis, two with cataract, two with infarction, one with transient optic neuropathy, and four with residual cranial nerve palsy, but none with glaucoma or dry eyes. In MRA analysis, total obliteration of the fistula was noted in 64 cases with no detectable ICA stenosis nor cavernous sinus thrombosis. In the Cox regression analysis, post-GKRS residual cranial nerve palsy was highly correlated to targeted volume (p < 0.05) and age (p < 0.05). The occurrence of post-GKRS cataract was related to the initial symptom of chemosis (p < 0.05). Conclusion: GKRS for carotid cavernous sinus fistula offers a high obliteration rate and preserves the cavernous sinus vascular structure while conferring a low risk of treatment complications such as adverse radiation risk to the optic apparatus and adjacent cranial nerves.