bluuebunny/crossref_metadata_2025 huggingface dataset:
A lightweight network for improving wheat ears detection and counting based on YOLOv5s
Authors: Shen Xiaojun , Zhang Chu , Liu Kai , Mao Wenjie , Zhou Cheng , Yao Lili
Published: December 2023

Introduction

Recognizing wheat ears plays a crucial role in predicting wheat yield. Employing deep learning methods for wheat ears identification is the mainstream method in current research and applications. However, such methods still face challenges, such as high computational parameter volume, large model weights, and slow processing speeds, making it difficult to apply them for real-time identification tasks on limited hardware resources in the wheat field. Therefore, exploring lightweight wheat ears detection methods for real-time recognition holds significant importance.

Methods

This study proposes a lightweight method for detecting and counting wheat ears based on YOLOv5s. It utilizes the ShuffleNetV2 lightweight convolutional neural network to optimize the YOLOv5s model by reducing the number of parameters and simplifying the complexity of the calculation processes. In addition, a lightweight upsampling operator content-aware reassembly of features is introduced in the feature pyramid structure to eliminate the impact of the lightweight process on the model detection performance. This approach aims to improve the spatial resolution of the feature images, enhance the effectiveness of the perceptual field, and reduce information loss. Finally, by introducing the dynamic target detection head, the shape of the detection head and the feature extraction strategy can be dynamically adjusted, and the detection accuracy can be improved when encountering wheat ears with large-scale changes, diverse shapes, or significant orientation variations.

Results and discussion

This study uses the global wheat head detection dataset and incorporates the local experimental dataset to improve the robustness and generalization of the proposed model. The weight, FLOPs and mAP of this model are 2.9 MB, 2.5 * 109 and 94.8%, respectively. The linear fitting determination coefficients R2 for the model test result and actual value of global wheat head detection dataset and local experimental Site are 0.94 and 0.97, respectively. The improved lightweight model can better meet the requirements of precision wheat ears counting and play an important role in embedded systems, mobile devices, or other hardware systems with limited computing resources.
What helps the helpers? Resilience and risk factors for general and profession-specific mental health problems in psychotherapists during the COVID-19 pandemic
Authors: Zerban Matthias , Puhlmann Lara Marie Christine , Lassri Dana , Fonagy Peter , Montague P. Read , Kiselnikova Natalia , Lorenzini Nicolas , Desatnik Alex , Kalisch Raffael , Nolte Tobias
Published: December 2023

Introduction

Although the COVID-19 pandemic has severely affected wellbeing of at-risk groups, most research on resilience employed convenience samples. We investigated psychosocial resilience and risk factors (RFs) for the wellbeing of psychotherapists and other mental health practitioners, an under-researched population that provides essential support for other at-risk groups and was uniquely burdened by the pandemic.

Method

We examined 18 psychosocial factors for their association with resilience, of which four were chosen due to their likely relevance specifically for therapists, in a cross-sectional multi-national sample (N = 569) surveyed between June and September 2020. Resilience was operationalized dimensionally and outcome-based as lower stressor reactivity (SR), meaning fewer mental health problems than predicted given a participant’s levels of stressor exposure. General SR (SRG) scores expressed reactivity in terms of general internalizing problems, while profession-specific SR (SRS) scores expressed reactivity in terms of burnout and secondary trauma, typical problems of mental health practitioners.

Results

Factors previously identified as RFs in other populations, including perceived social support, optimism and self-compassion, were almost all significant in the study population (SRG: 18/18 RFs, absolute βs = 0.16–0.40; SRS: 15/18 RFs, absolute βs = 0.19–0.39 all Ps < 0.001). Compassion satisfaction emerged as uniquely relevant for mental health practitioners in regularized regression.

Discussion

Our work identifies psychosocial RFs for mental health practitioners’ wellbeing during crisis. Most identified factors are general, in that they are associated with resilience to a wider range of mental health problems, and global, in that they have also been observed in other populations and stressor constellations.
Health-related quality of life in family caregivers of autistic adults
Authors: Dückert Sophia , Bart Sabine , Gewohn Petia , König Hannah , Schöttle Daniel , Konnopka Alexander , Rahlff Pascal , Erik Frank , Vogeley Kai , Schulz Holger , David Nicole , Peth Judith
Published: December 2023

Introduction

Family members of autistic individuals often provide support for their autistic relative throughout the lifespan which can lead to massive burden themselves. Reduced health-related Quality of Life (HRQoL) in family caregivers is assumed; however, only a handful studies on the HRQoL of family caregivers providing care to adult relatives exist as opposed to autistic children. Thus, the current study aimed to (i) investigate the current state of physical and mental HRQoL of family caregivers of autistic adults compared to the general population, and (ii) examine caregiver-related (e.g., age, subjective caregiver burden) and care recipient-related variables (e.g., symptom severity, utilization of formal services) explaining variance in the caregivers’ HRQoL.

Methods

N = 149 family caregivers completed a nationwide online survey, including the Short-Form Health Survey (SF-8) in order to assess the HRQoL. T-tests were used to compare the HRQoL of family caregivers with the general population. Bivariate correlational and multiple linear regression analyses were conducted in order to identify predictors explaining variance in family caregivers’ HRQoL.

Results

Family caregivers of autistic adults reported significantly lower physical (M = 46.71, SD = 8.72, Cohen’s d = 0.42) and mental HRQoL (M = 40.15, SD = 11.28, Cohen’s d = 1.35) compared to the general population. Multiple linear regression with the mental HRQoL as the outcome showed a significant model (F(11, 95) = 5.53, p < .001, adj. R2 = .32) with increased subjective burden explaining most of the variance in mental HRQoL (ß = .32, GDW = .141, p < .001). Multiple linear regression analysis with the outcome physical HRQoL did not reveal a statistically significant model (F(11,95) = 1.09, p = .38). However, bivariate analyses also showed a positive correlation with the subjective caregiver burden (r= .20, p < .05).

Discussion

Findings highlight the need to consider HRQoL (and caregiver burden) of family caregivers of autistic adults in several healthcare settings to monitor a potential comprised health status in early stages, with the long-term goal to improve family caregivers’ HRQoL.
Metabolic syndrome in adults with autistic traits: associated psychological, behavioral, and biological factors in females and males – a PharmLines initiative
Authors: Warreman E. B. , Nooteboom L. A. , Leenen P. J. M. , Geurts H. M. , Terry M. B. , Bos J. H. J. , Hak E. , Hoek H. W. , van Rossum E. F. C. , Vermeiren R. R. J. M. , Ester W. A.
Published: December 2023

Background

While cardiovascular diseases is highly prevalent and an important cause of mortality in autistic adults, knowledge on their increased cardiovascular risk is limited. Hence, this study aimed to investigate psychological, behavioral, and physical factors associated with metabolic syndrome (MetS) in adults with autistic traits.

Methods

In total, 17,705 adults from the Lifelines Cohort were included and categorized using Autism Spectrum Quotient-10 sum-scores. The quartiles with highest (HQ-traits-group females: n = 2,635; males: n = 1803) and lowest levels of autistic traits (LQ-traits-group, n = idem) were analyzed. Using multivariable logistic regression, the associations between MetS and (self-reported and interviewed) psychological, behavioral, and physically measured factors in these stratified groups were investigated.

Results

Among females, MetS was more common in the HQ-traits-group than in the LQ-traits-group (10.0% versus 7.5%, p < 0.01), while this was not the case among males (HQ-traits-group 13.8% versus LQ-traits-group 13.1%, p = 0.52). In both the female and male HQ-traits-group, the presence of MetS was associated with poorer self-reported health, less daily physical activity, and altered leukocyte counts.

Conclusion

These findings underline the relevance of adequate cardiovascular prevention in adults with higher levels of autistic traits. Future research could gain more insight into the relationship between cardiovascular risk and autistic traits in females, and into tailored cardiovascular prevention.
Environmental factors of obesity before and after COVID-19 pandemic: a review
Authors: Wolińska Irena Anna , Kraik Krzysztof , Poręba Rafał , Gać Paweł , Poręba Małgorzata
Published: December 2023

In past decades the prevalence of overweight and obesity had grown rapidly. There are numerous factors contributing to this unfavorable change in people’s health. This review article investigates the environmental factors which may play a role in the prevalence of overweight and obesity and additionally the novel factors which appeared after the beginning of the COVID-19 pandemic, which caused the increase in BMI during the lockdown period. Most of the studies reveal that the COVID-19 pandemic and lockdown contributed to the growth of BMI in numerous countries and, eventually the prevalence of overweight and obesity increased. Studies suggest that the physical activity was decreased while sleep time and screen time were increased and the amount of food consumed increased, additionally more processed food with long shelf life was consumed. The diverse environmental factors may have an impact on obesity and overweight development taking into account policy and local school policy issues, socioeconomic status, lifestyle including physical activity, diet habits, and amongst others, more trivial causes such as uninteresting neighborhoods, lack of sense of security outside the place of residence or a long distance from shops. Still, this is the object of debate if air pollution is an environmental risk factor influencing the unfavorable trends towards increasing body weight.
Factors associated with grazing behavior in candidates for bariatric surgery at a hospital in the Amazon
Authors: Kikuchi Jeane Lorena Dias , Carvalhal Manuela Maria de Lima , de Castro Mariana Maués , Vieira Lourenço-Costa Vanessa , dos Santos Carlos Armando Ribeiro , Cunha Flávia Siqueira , Paracampo Carla Cristina Paiva , Gomes Daniela Lopes
Published: December 2023

Blackground

To assess grazing behavior and associated factors in candidates for bariatric surgery monitored at a public hospital that is a reference in the care of people with severe obesity.

Methods

Cross-sectional analytical study, with candidates for bariatric surgery of both genders, treated in a public hospital in the Amazon. To assess grazing behavior, the Repetitive Eating Questionnaire was used, and to investigate patterns of eating behavior, the Three Factor Eating Questionnaire was used, which assesses: Emotional Eating, Cognitive Restriction and Uncontrolled Eating. Sociodemographic information was obtained through self-report and the description of medication use through the medical record. Body mass index (BMI) was also calculated by measuring weight and height. The SPSS program, v. 21.0 was used. The study was approved by the Research Ethics Committee.

Results

A total of 205 participants were evaluated, with a mean age of 37.5 ± 8.6 years, the majority (93.7%) being women and the majority (59.5%) was not also using medication to lose weight. About 66.3% of the participants had compulsive grazing. The factor with the highest score was cognitive restriction (p < 0.001). Individuals who used weight loss drugs had higher scores in the cognitive restriction factor (p = 0.015) and lower scores for uncontrolled eating (p = 0.008), compulsive grazing (p = 0.021) and non-compulsive grazing (p = 0.034).

Conclusion

Linear regression showed that emotional eating and uncontrolled eating were predictors of both compulsive grazing and non-compulsive grazing behavior. It was observed that grazing behavior, cognitive restriction, emotional eating and uncontrolled eating are present and correlated in the studied patients. In addition, the use of weight loss drugs seems to help reduce dysfunctional eating behaviors in patients with severe obesity.
Association between kidney function and biological age: a China Health and Retirement Longitudinal Study
Authors: Peng Shanshan , Xu Rui , Wei Kai , Liu Na , Lv Yuan , Lin Yong
Published: December 2023

Introduction

The chronological age (CA) cannot precisely reflect the health status. Our study aimed to establish a model of kidney biological age to evaluate kidney function more elaborately.

Methods

The modeling group was used to establish the model, consisting of 1,303 respondents of the China Health and Retirement Longitudinal Study (CHARLS). The biological age of the kidney (BA) was constructed by principal component analysis (PCA) and Klemera and Doubal’s method (KDM) with the 1,303 health respondents.

Results

PCA was chosen as the best method for our research step by step. The test group was used to apply the model. (a) BA of the kidney can distinguish respondents with from without kidney disease. (b) BA of the kidney was significantly different in various levels of kidney function. The BA of the eGFR <60 group and 60 ≤ eGFR <90 group were older than GFR ≥90 group. (c) The group with younger BA of kidney at baseline had a lower risk of kidney function decreased. (d) The risk of decreased kidney function caused by increasing BA every additional year is higher than CA.

Discussion

The BA of the kidney is a parameter negatively correlated with decreased kidney function and fills the blank of evaluation among people in the middle of heathy and kidney diseases.
Prediabetes prevalence and awareness by race, ethnicity, and educational attainment among U.S. adults
Authors: Formagini Taynara , Brooks Joanna Veazey , Roberts Andrew , Bullard Kai McKeever , Zhang Yan , Saelee Ryan , O'Brien Matthew James
Published: December 2023

Introduction

Racial and ethnic minority groups and individuals with limited educational attainment experience a disproportionate burden of diabetes. Prediabetes represents a high-risk state for developing type 2 diabetes, but most adults with prediabetes are unaware of having the condition. Uncovering whether racial, ethnic, or educational disparities also occur in the prediabetes stage could help inform strategies to support health equity in preventing type 2 diabetes and its complications. We examined the prevalence of prediabetes and prediabetes awareness, with corresponding prevalence ratios according to race, ethnicity, and educational attainment.

Methods

This study was a pooled cross-sectional analysis of the National Health and Nutrition Examination Survey data from 2011 to March 2020. The final sample comprised 10,262 U.S. adults who self-reported being Asian, Black, Hispanic, or White. Prediabetes was defined using hemoglobin A1c and fasting plasma glucose values. Those with prediabetes were classified as “aware” or “unaware” based on survey responses. We calculated prevalence ratios (PR) to assess the relationship between race, ethnicity, and educational attainment with prediabetes and prediabetes awareness, controlling for sociodemographic, health and healthcare-related, and clinical characteristics.

Results

In fully adjusted logistic regression models, Asian, Black, and Hispanic adults had a statistically significant higher risk of prediabetes than White adults (PR:1.26 [1.18,1.35], PR:1.17 [1.08,1.25], and PR:1.10 [1.02,1.19], respectively). Adults completing less than high school and high school had a significantly higher risk of prediabetes compared to those with a college degree (PR:1.14 [1.02,1.26] and PR:1.12 [1.01,1.23], respectively). We also found that Black and Hispanic adults had higher rates of prediabetes awareness in the fully adjusted model than White adults (PR:1.27 [1.07,1.50] and PR:1.33 [1.02,1.72], respectively). The rates of prediabetes awareness were consistently lower among those with less than a high school education relative to individuals who completed college (fully-adjusted model PR:0.66 [0.47,0.92]).

Discussion

Disparities in prediabetes among racial and ethnic minority groups and adults with low educational attainment suggest challenges and opportunities for promoting health equity in high-risk groups and expanding awareness of prediabetes in the United States.
Renewed calls for abortion-related research in the post-Roe era
Authors: Schott Sophie L. , Adams April , Dougherty Ryan J. , Montgomery Taylor , Lapite Folasade C. , Fletcher Faith E.
Published: December 2023

Nearly 50 years after Roe versus Wade, the United States Supreme Court’s decision in Dobbs versus Jackson Women’s Health Organization unraveled the constitutional right to abortion, allowing individual states to severely restrict or ban the procedure. In response, leading medical, public health, and community organizations have renewed calls for research to elucidate and address the burgeoning social and medical consequences of new abortion restrictions. Abortion research not only includes studies that establish the safety, quality, and efficacy of evidence-based abortion care protocols, but also encompasses studies on the availability of abortion care, the consequences of being denied an abortion, and the legal and social burdens surrounding abortion. The urgency of these calls for new evidence underscores the importance of ensuring that research in this area is conducted in an ethical and respectful manner, cognizant of the social, political, and structural conditions that shape reproductive health inequities and impact each stage of research—from protocol design to dissemination of findings. Research ethics relates to the moral principles undergirding the design and execution of research projects, and concerns itself with the technicalities of ethical questions related to the research process, such as informed consent, power relations, and confidentiality. Critical insights and reflections from reproductive justice, community engagement, and applied ethics frameworks have bolstered existing research ethics scholarship and discourse by underscoring the importance of meaningful engagement with community stakeholders—bringing attention to overlapping structures of oppression, including racism, sexism, and ways that these structures are perpetuated in the research process.
Peripheral nerve stimulation for pudendal neuralgia and other pelvic pain disorders: current advances
Authors: Kovacevic Natalija , Sirls Larry , Gilleran Jason , Peters Kenneth
Published: December 2023

Chronic pelvic pain conditions such as pudendal neuralgia pose significant treatment difficulty due to their elusive etiology and diverse symptomatology. Initially approved as a third or fourth-line treatment of non-obstructive urinary retention and fecal incontinence, neuromodulation has also proven effective for pelvic pain associated with urinary dysfunction. Recently, sacral and pudendal neuromodulation has demonstrated efficacy in managing a spectrum of chronic pelvic conditions including refractory pudendal neuralgia. The individualized approach of peripheral neuromodulation has opened new avenues for tailored medical interventions, extending its application to conditions such as pudendal neuralgia, post sling pain, and vulvodynia. New technologies leading to miniaturized neuromodulation devices such as Freedom® stimulators (Curonix), allows us to implant leads and modulate nerves at precise pain targets. Further experience and research is needed to assess the impact of targeted neuromodulation on managing complex pelvic pain conditions.
An ecotoxicologically relevant approach to water quality monitoring for contaminants of emerging concern
Authors: Schoenfuss Heiko L. , Kolok Alan S.
Published: December 2023

Contaminants of Emerging Concern (CECs) have been documented across the seven continents, including Antarctica, and are likely an impediment to the sustainable management of natural resources. Most studies to date have relied on sweeping chemistry surveys, reliant upon sophisticated instrumentation. This approach is expensive, relies on limited laboratory capacity, and generates results that are spatially and temporally constrained. Here we review existing approaches that can overcome these limitations by focusing on effects-based monitoring. Passive samplers can generate long-term records regarding the occurrence of CECs. As samples are concentrated, their analysis can be achieved using equipment that will be more common and less expensive. A second approach involves rapid test methods for single compounds, including test strips, ELISA assays, and mobile phone-based analytic tools. These can provide inexpensive CEC presence data for many field sites and can be used to stratify sampling and thereby reduce cost. Identifying the presence of a single compound can often shed light on the likely presence of entire groups of chemicals. Pairing these chemistry-derived approaches with geospatial modeling to predict CEC presence and concentrations across watersheds has already been applied in several large watersheds. Utilizing available ecotoxicological knowledge bases provides an opportunity to link modeled CEC occurrence and concentrations with likely adverse biological responses. Finally, confirmatory on-site exposure experiments can corroborate the presence or absence of biological effects hypothesized from the above chain of evidence to provide natural resource managers with information to make conservation decisions.
Promoting quality in low-cost gas sensor devices for real-world applications
Authors: Reimringer Wolfhard , Bur Christian
Published: December 2023

Recent advancements in metal oxide semiconductor sensors enable system integrators to make sensor devices without the traditional complications of operating barebone sensor components. Anyone, so it seems, can put together multi-sensor-systems by combining an integrated sensor subsystem with a small piece of digital infrastructure, resulting in low-cost sensor systems or sensor networks for all kinds of applications. With low energy consumption, the deployment of close meshed sensor networks is becoming a reality, promising high density data for big data models. However, data quality is not necessarily a feature of such devices, since the device output signals are heavily processed and the insight into the actual operating technique is black-boxed by the respective sensor manufacturer. High volume production of a sensor model requires widely applicable output information, yet this mainstreaming negatively impacts quality control efforts on the system level as well as niche applications that require specialized operation modes. This article exemplarily examines the measurement chain of typical metal oxide semiconductor sensor applications and deduces requirements for a technically sound advancement of the subject for research and commercial purposes. Equivalent considerations can be made for any low-cost sensor principle and their respective challenges. The conclusion urges all stakeholders participating in the development and marketing of sensor devices to advance a scientifically valid state of knowledge and to educate their customers and even the general public accordingly.
Injuries in male youth football: a one season prospective cohort study of 223 Danish elite players
Authors: Andersen Thomas Rostgaard , Drevsfeldt Andreas , Möller Sören , Møller Merete
Published: December 2023

Objectives

This study prospectively investigated injury prevalence, incidence, and burden in male elite under-17 football players (N = 223)during a full season.

Methods

The players weekly completed a standardized web-based injury survey (OSTRC-H2) and a physical exposure report throughout the study.

Results

Average weekly response rate was 89.5%. Football exposure accounted for 52.4% of total physical exposure. On average (±SD), the players participated in individual football, strength, and rehabilitation practices for 1.2 ± 1.5, 3.0 ± .2.1, and 1.9 ± 3.4 h/week, respectively. In total, 742 health problems were reported. Mean weekly prevalence of health problems, injuries and illnesses were 20.1%, 16.5% and 3.8%, respectively. The injury incidence per 1,000 h of football exposure, match play and team practice were 8.28 (95% CI: 7.54–9.08), 16.77 (95% CI: 13.65–20.4), and 7.24 (95% CI: 6.5–8.04), respectively. Sudden-onset and gradual-onset injuries accounted for 36.7% and 43.4% of the total proportion of health problems. Hip/groin injuries had the highest incidence (1.58/1,000 h), whereas knee injuries had the highest burden (20.86 days lost/1,000 h). On average, the players experienced 3.33 health problems (average duration: 7.8 days). On average pr. player, 2.7 (95% CI: 2.2–3.3) wks of football exposure were lost.

Conclusion

Sudden and gradual-onset injuries influenced player availability during the season. Health problem prevalence fluctuated markedly, and injury incidence was higher during match play than training. The players had substantial volumes of training beyond football-specific training and matches. Our findings could assist medical and sports science practitioneers in enhancing training and recovery processes to maximize player availability.
Percutaneous closure of subclavian iatrogenic injuries after central venous catheterization: a Latin American experience
Authors: Rey Chaves Carlos Eduardo , Orozco Claudia , Posada Eduardo , Gómez Zuleta María , Fajardo Ernesto , Barón Vladimir , Hernández Rodríguez Oscar Geovanny
Published: December 2023

Introduction

Arterial injuries following central venous catheterization (CVC) range between 0.1%–2.7%. The open surgical approach could be related to increased rates of morbidity and mortality. Vascular closure devices (VCD) are often used for the management of these patients with a success rate of up to 80%.

Objectives

Describe our experience in managing arterial vascular injuries following central venous catheterization with Perclose ProGlide (Abbott Vascular IncSanta Clara, CA, USA).

Methods

A retrospective review of all patients over 18 years old who underwent percutaneous closure of arterial injuries following central venous catheterization in our center between January 2018 and May 2023 was included and reported with a 90-day follow-up.

Results

3 Patients were included, in all cases, access to the CVC were right with a subclavian artery injury. Ultrasound and fluoroscopy guide was used in all cases. For the 3 cases, a percutaneous technique using Perclose ProGlide (Abbott Vascular IncSanta Clara, CA, USA) was performed. With a 100% success rate, and no complications evidenced after 90 days of follow-up.

Conclusion

Inadvertent arterial catheterization it's a non-negligible complication after CVC placement. VCD could be considered a safe and feasible approach for the management of these traumatic injuries.
Meckel's diverticulum: analysis of 27 cases in an adult population
Authors: Hernández Juan David , Valencia Gustavo , Girón Felipe , García Sierra Andrés Mauricio , Núñez-Rocha Ricardo E. , Rodríguez Lina M. , Rey Chaves Carlos Eduardo , Londoño Eduardo Emilio , Nassar Ricardo
Published: December 2023

Background

Meckel's diverticulum is a rare congenital pathology among newborns. Nevertheless, it is an uncommon abdominal pathology in the adult population. Therefore, we aim to provide a detailed account of our surgical approach in treating 27 cases of Meckel's diverticulum.

Methods

This study is a cross-sectional analysis that utilized a database with prospectively collected data from 2004 to 2022. All patients under the age of 18 were excluded from the population. We described the population’s demographic characteristics, symptoms, anatomopathological study, surgical technique, complications, morbidity, and mortality. A subgroup analysis was performed between the symptomatic and asymptomatic patients.

Results

A total of 27 patients who underwent surgical resection for a posteriorly diagnosed Meckel's diverticulum were included. The male population accounted for 81.4% (n = 22) of the sample size. The symptomatic group consisted of 18 male and four female patients. Abdominal pain was the predominant symptom in 85% of the patients. Out of the 22 symptomatic patients, only 9% had a positive perioperative diagnosis of Meckel's diverticulum. All 27 patients with diverticulum diagnosis received the resection through diverticulectomy (n = 6), small bowel resection with end-to-end anastomosis (n = 6), and small bowel resection with lateral to lateral anastomosis (n = 15). The mean distance between the diverticulum and the ileocecal valve was 63.4 cm. The symptomatic group had an average diverticulum length of 3.54 cm, with an average base width of 2.47 cm. In the other group, the values were 2.75 and 1.61 cm. The average length of hospital stay in the symptomatic group was 7.3 days.

Conclusions

Meckel's diverticulum is a rare pathology in the adult population. Its presentation varies from asymptomatic to symptomatic patients, and surgery is the cornerstone treatment for this pathology.
Evolutionary Algorithms in a Bacterial Consortium of Synthetic Bacteria
Authors: Villaescusa Sara Lledó , Lahoz-Beltra Rafael
Published: December 2023

At present, synthetic biology applications are based on the programming of synthetic bacteria with custom-designed genetic circuits through the application of a top-down strategy. These genetic circuits are the programs that implement a certain algorithm, the bacterium being the agent or shell responsible for the execution of the program in a given environment. In this work, we study the possibility that instead of programming synthesized bacteria through a custom-designed genetic circuit, it is the circuit itself which emerges as a result of the evolution simulated through an evolutionary algorithm. This study is conducted by performing in silico experiments in a community composed of synthetic bacteria in which one species or strain behaves as pathogenic bacteria against the rest of the non-pathogenic bacteria that are also part of the bacterial consortium. The goal is the eradication of the pathogenic strain through the evolutionary programming of the agents or synthetic bacteria. The results obtained suggest the plausibility of the evolutionary design of the appropriate genetic circuit resulting from the application of a bottom-up strategy and therefore the experimental feasibility of the evolutionary programming of synthetic bacteria.
Coffee Growing with Remotely Piloted Aircraft System: Bibliometric Review
Authors: Bento Nicole Lopes , Ferraz Gabriel Araújo e Silva , Santana Lucas Santos , Silva Mirian de Lourdes Oliveira e
Published: December 2023

Remotely piloted aircraft systems (RPASs) have gained prominence in recent decades primarily due to their versatility of application in various sectors of the economy. In the agricultural sector, they stand out for optimizing processes, contributing to improved sampling, measurements, and operational efficiency, ultimately leading to increased profitability in crop production. This technology is becoming a reality in coffee farming, an essential commodity in the global economic balance, mainly due to academic attention and applicability. This study presents a bibliometric analysis focused on using RPASs in coffee farming to structure the existing academic literature and reveal trends and insights into the research topic. For this purpose, searches were conducted over the last 20 years (2002 to 2022) in the Web of Science and Scopus scientific databases. Subsequently, bibliometric analysis was applied using Biblioshiny for Bibliometrix software in R (version 2022.07.1), with emphasis on the temporal evolution of research on the topic, performance analysis highlighting key publications, journals, researchers, institutions, countries, and the scientific mapping of co-authorship, keywords, and future trends/possibilities. The results revealed 42 publications on the topic, with the pioneering studies being the most cited. Brazilian researchers and institutions (Federal University of Lavras) have a strong presence in publications on the subject and in journals focusing on technological applications. As future trends and possibilities, the employment of technology optimizes the productivity and profitability studies of coffee farming for the timely and efficient application of aerial imaging.
Consumption of Rodenticide Baits by Invertebrates as a Potential Route into the Diet of Insectivores
Authors: Williams Emily J. , Cotter Sheena C. , Soulsbury Carl D.
Published: December 2023

Non-target species are commonly exposed to anticoagulant rodenticides worldwide, which may pose a key threat to declining species. However, the main pathway of exposure is usually unknown, potentially hindering conservation efforts. This study aimed to examine whether baits mixed with the biomarker rhodamine B can be used to track invertebrate consumption of rodenticides in a field environment, using this to observe whether invertebrate prey are a potential vector for anticoagulant rodenticides in the diet of insectivores such as the European hedgehog (Erinaceus europaeus). Rhodamine B baits were found to create an observable response. Uptake was negligible in captured insects; however, 20.7% of slugs and 18.4% of snails captured showed uptake of bait. Maximum temperature, distance from bait, proximity to buildings, and the addition of copper tape to bait boxes all influenced the rate of bait uptake in molluscs. Based on these data, it seems likely that molluscs could be a source of rodenticide poisoning in insectivores. This research demonstrates which prey may pose exposure risks to insectivores and likely environmental factors, knowledge of which can guide effective mitigation measures. We suggest that further investigation into using mollusc repellents around bait boxes should be considered.
Effects of the Addition of Crude Fibre Concentrate on Performance, Welfare and Selected Caecal Bacteria of Broilers
Authors: Urban Jakub , Jaworski Sławomir , Lange Agata , Bień Damian , Matuszewski Arkadiusz , Michalczuk Monika
Published: December 2023

The study evaluated the effects of crude fibre concentrate supplementation on final body weight, mortality, feed conversion ratio, European Production Efficiency Factor, European Broiler Index, welfare parameters, colony-forming units of selected caecal bacteria (Enterobacteriaceae and lactic acid bacteria) and pH of broiler faeces and litter. The study comprised 990 Ross 308 male chicks divided into three groups, a control and two experimental groups, which were given crude fibre concentrate as a feed supplement. On the thirty-fifth day of rearing, the birds’ welfare scores were evaluated, and 2 g of cecum was collected post-mortem from six chickens in each group. Subsequently, a series of ten-fold dilutions of the material was prepared, followed by cultures and measurement of pH in the faeces and litter. The inclusion of crude fibre concentrate resulted in a beneficial impact on the ultimate body mass (p ≤ 0.001), welfare standard (p ≤ 0.001), and quantity of colony-forming units of lactic acid bacteria (p ≤ 0.05) within the cecum. Furthermore, it had a positive influence on lowering the pH levels of both faeces and litter (p ≤ 0.05).
Lingual Ultrastructural and Histochemical Study in the Patagonian Mara (Rodentia: Caviidae, Dolichotis patagonum) in Relation to Other Hystricomorphs
Authors: Čížek Petr , Goździewska-Harłajczuk Karolina , Hamouzová Pavla , Klećkowska-Nawrot Joanna , Kvapil Pavel
Published: December 2023

The study describes the ultrastructure of the tongue in the Patagonian mara (Dolichotis patagonum) using light and scanning electron microscopy. Histochemical methods were used for evaluation of lingual salivary glands. The tongue is divided into a small and rounded apex, a narrow corpus, and a significantly wider radix, with a well-developed lingual prominence. The tip of the apex is free of papillae. The caudal part of the apex and the corpus are covered by filiform papillae. Round fungiform papillae are scattered among them. Papillae on the narrow stalk are conical. The radix contains caudally bent papillae forming wide flat prominences, slender, hook shaped filiform papillae, foliate papillae, and two oval vallate papillae. Taste buds were found on the lateral sides of the foliate and vallate papillae. Purely serous salivary glands are beneath the vallate and foliate papillae. Serous acini and mucous tubules are in the lingual radix. The Patagonian mara is the only hystricomorph rodent with described hyaline cartilage strengthening the lingual radix. Some typical signs of adaptation to herbivorous diet were found. The structure of the tongue is adapted to grass-feeding, as grasses form the main component of their diet.
Comparison of PBL Heights from Ceilometer Measurements and Greenhouse Gases Concentrations in São Paulo
Authors: Vieira dos Santos Amanda , Cristina Araújo Elaine , da Silva Andrade Izabel , Corrêa Thais , Talita Amorim Marques Márcia , Eduardo Souto-Oliveira Carlos , Franchi Leonardo Noele , de Mendonça Macedo Fernanda , Souza Giovanni , Pereira de Queiroz Lopes Pérola , de Arruda Moreira Gregori , de Fátima Andrade Maria , Landulfo Eduardo
Published: December 2023

This paper presents a study conducted in São Paulo, Brazil, where the planetary boundary layer height (PBLH) was determined using ceilometer data and the wavelet covariance transform method. The retrieved PBLH values were subsequently compared with the concentrations of CO2 and CH4 measured at three distinct experimental sites in the city. The period of study was July 2021. This study also included a comparison between ceilometer data and lidar data, which demonstrated the favorable applicability of the ceilometer data for PBLH estimation. An examination of the correlation between changes in average CO2 concentrations and PBLH values revealed stronger correlations for the IAG and UNICID stations, with correlation coefficients (ρ) of approximately −0.86 and −0.85, respectively, in contrast to the Pico do Jaraguá station, which exhibited a lower correlation coefficient of −0.42. When assessing changes in CH4 concentrations against variations in PBL height, the retrieved correlation coefficients were approximately −0.78 for IAG, −0.66 for UNICID, and −0.38 for Pico do Jaraguá. The results indicated that CO2/CH4 concentrations are negatively correlated with PBL heights, with CO2 concentrations showing more significant correlation than CH4. Additionally, among the three measurement stations, IAG measurements displayed the most substantial correlation. The results from this study contribute to the understanding of the relationship between PBLH and greenhouse gas concentrations, emphasizing the potential of remote sensing systems like ceilometers in monitoring and studying atmospheric processes.
Air Purification Study Based on the Adhesion Effect between Low-Curvature Liquid Surfaces and Air Convection Friction
Authors: Weng Haotian , Zhang Yaozhong , Huang Xiaolu , Yuan Hewei , Zhang Yafei
Published: December 2023

Rapid urbanization and industrialization have heightened concerns about air quality worldwide. Conventional air purification methods, reliant on chemicals or energy-intensive processes, fall short in open spaces and in combating emerging pollutants. Addressing these limitations, this study presents a novel water-film air purification prototype leveraging the adhesion between low-curvature liquid surfaces and air convection friction. Uniquely designed, this prototype effectively targets toxic gases (e.g., formaldehyde, SO2, NO2) and particulate matter (such as PM2.5) while allowing continuous airflow. This research explores the adhesion and sedimentation capabilities of a low-curvature water solution surface under convection friction, reducing the surface energy to remove airborne pollutants efficiently. The prototype was able to reduce the initial concentration in a 30 m³ chamber within 180 min by 91% for formaldehyde, 78% for nitrogen dioxide (NO2), 99% for sulfur dioxide (SO2), and 96% for PM2.5. Experimentally validated indicators—decay constants, CADR, and purification efficiency—enable a comprehensive evaluation of the purification device, demonstrating its efficacy in mitigating air pollution. This innovative design, which is cost-effective due to its use of easily accessible components and water as the primary medium, indicates strong potential for large-scale deployment. This study points to an environmentally friendly and economical approach to air purification, shedding light on a promising direction for enhancing indoor air quality. Further optimization and exploration of diverse pollutants and environmental conditions will propel the practical applications of this pioneering technology.
Electroconductive Nanofibrous Scaffolds Enable Neuronal Differentiation in Response to Electrical Stimulation without Exogenous Inducing Factors
Authors: Ranjbar Nika , Bakhshandeh Behnaz , Pennisi Cristian Pablo
Published: December 2023

Among the various biochemical and biophysical inducers for neural regeneration, electrical stimulation (ES) has recently attracted considerable attention as an efficient means to induce neuronal differentiation in tissue engineering approaches. The aim of this in vitro study was to develop a nanofibrous scaffold that enables ES-mediated neuronal differentiation in the absence of exogenous soluble inducers. A nanofibrous scaffold composed of polycaprolactone (PCL), poly-L-lactic acid (PLLA), and single-walled nanotubes (SWNTs) was fabricated via electrospinning and its physicochemical properties were investigated. The cytocompatibility of the electrospun composite with the PC12 cell line and bone marrow-derived mesenchymal stem cells (BMSCs) was investigated. The results showed that the PCL/PLLA/SWNT nanofibrous scaffold did not exhibit cytotoxicity and supported cell attachment, spreading, and proliferation. ES was applied to cells cultured on the nanofibrous scaffolds at different intensities and the expression of the three neural markers (Nestin, Microtubule-associated protein 2, and β tubulin-3) was evaluated using RT-qPCR analysis. The results showed that the highest expression of neural markers could be achieved at an electric field intensity of 200 mV/cm, suggesting that the scaffold in combination with ES can be an efficient tool to accelerate neural differentiation in the absence of exogenous soluble inducers. This has important implications for the regeneration of nerve injuries and may provide insights for further investigations of the mechanisms underlying ES-mediated neuronal commitment.
A Real-Time Control Method for Upper Limb Exoskeleton Based on Active Torque Prediction Model
Authors: Li Sujiao , Zhang Lei , Meng Qiaoling , Yu Hongliu
Published: December 2023

Exoskeleton rehabilitation robots have been widely used in the rehabilitation treatment of stroke patients. Clinical studies confirmed that rehabilitation training with active movement intentions could improve the effectiveness of rehabilitation treatment significantly. This research proposes a real-time control method for an upper limb exoskeleton based on the active torque prediction model. To fulfill the goal of individualized and precise rehabilitation, this method has an adjustable parameter assist ratio that can change the strength of the assist torque under the same conditions. In this study, upper limb muscles’ EMG signals and elbow angle were chosen as the sources of control signals. The active torque prediction model was then trained using a BP neural network after appropriately extracting features. The model exhibited good accuracy on PC and embedded systems, according to the experimental results. In the embedded system, the RMSE of this model was 0.1956 N·m and 94.98%. In addition, the proposed real-time control system also had an extremely low delay of only 40 ms, which would significantly increase the adaptability of human–computer interactions.
Biocatalysts Based on Immobilized Lipases for the Production of Fatty Acid Ethyl Esters: Enhancement of Activity through Ionic Additives and Ion Exchange Supports
Authors: Pardo-Tamayo Juan S. , Arteaga-Collazos Sebastián , Domínguez-Hoyos Laura C. , Godoy César A.
Published: December 2023

Ionic additives affect the structure, activity and stability of lipases, which allow for solving common application challenges, such as preventing the formation of protein aggregates or strengthening enzyme–support binding, preventing their desorption in organic media. This work aimed to design a biocatalyst, based on lipase improved by the addition of ionic additives, applicable in the production of ethyl esters of fatty acids (EE). Industrial enzymes from Thermomyces lanuginosus (TLL), Rhizomucor miehei (RML), Candida antárctica B (CALB) and Lecitase®, immobilized in commercial supports like Lewatit®, Purolite® and Q-Sepharose®, were tested. The best combination was achieved by immobilizing lipase TLL onto Q-Sepharose® as it surpassed, in terms of %EE (70.1%), the commercial biocatalyst Novozyme® 435 (52.7%) and was similar to that of Lipozyme TL IM (71.3%). Hence, the impact of ionic additives like polymers and surfactants on both free and immobilized TLL on Q-Sepharose® was assessed. It was observed that, when immobilized, in the presence of sodium dodecyl sulfate (SDS), the TLL derivative exhibited a significantly higher activity, with a 93-fold increase (1.02 IU), compared to the free enzyme under identical conditions (0.011 IU). In fatty acids ethyl esters synthesis, Q-SDS-TLL novel derivatives achieved results similar to commercial biocatalysts using up to ~82 times less enzyme (1 mg/g). This creates an opportunity to develop biocatalysts with reduced enzyme consumption, a factor often associated with higher production costs. Such advancements would ease their integration into the biodiesel industry, fostering a greener production approach compared to conventional methods.
Temporal Shift Length and Antecedent Occurrence Likelihood Modulate Counterfactual Conditional Comprehension: Evidence from Event-Related Potentials
Authors: Kong Lingda , Jiang Yong , Huang Yan , Jiang Xiaoming
Published: December 2023

Counterfactual conditionals posit hypothetical scenarios in which antecedent events contradict reality. This study examined whether and how the processing difficulty of Chinese counterfactual conditionals (yaobushi, equivalent to if it had not been for in English) can be affected by the length of temporal shifts of the events across clauses and the likelihood of the antecedent occurrence. Participants read Chinese counterfactuals that contained either long (e.g., qunian-xianzai [last year-right now]) or short temporal shifts (e.g., zuotian-xianzai [yesterday-right now]) within highly likely (e.g., sign up for school activity) or less likely contexts (e.g., sign up for Arctic scientific research). ERP results revealed a significant N400 interaction between the temporal shift length and antecedent likelihood on the temporal indicators in the consequent and the sentence-ending verbs. Specifically, the less likely events elicited larger negativity than highly likely events with short temporal shifts on the temporal indicator. On the sentence-ending word, the long temporal shift elicited enlarged negativity than the short temporal shift when the antecedent was highly likely. These findings have two key implications regarding the interplay of implied causality and falsity constraints during counterfactual comprehension. First, salient falsity constraints can override effects of causal coherence on processing. Second, greater negativity for unlikely antecedents suggests that counterfactual markers concurrently activate factual and hypothetical representations.