bluuebunny/crossref_metadata_2025 huggingface dataset:
Design of an Integrated Platform for Active Debris Removal
Authors: Lv Senwei , Zhang Haojun , Zhang Yao , Ning Bowen , Qi Rui
Published: June 2022

In research concerning active debris removal, scholars have proposed dozens of schemes for removing debris. However, every scheme has both advantages and disadvantages, and no scheme possesses an overwhelming advantage. This paper proposes an integrated platform scheme which integrates multiple capture and deorbit technologies, such as a tethered net, harpoon, and robotic arm, to improve the success rate in terms of the active removal of debris of different shapes and different sizes. The design of the mechanisms of the integrated platform is presented in detail.
Adverse Effects of Long-Term Continuous Girdling of Jujube Tree on the Quality of Jujube Fruit and Tree Health
Authors: Ran Junhui , Guo Wensong , Hu Can , Wang Xufeng , Li Ping
Published: June 2022

In order to improve fruit yield, girdling technology is widely used in fruit production. However, in the practice of Hui jujube planting in Southern Xinjiang, it was found that the jujube fruit quality declined and the health problems and death rate of jujube trees increased after jujube trees have been girdled continuously for many years, which seriously affected the economic benefits of jujube planting. So, this study was carried out to explore the specific influence law of continuously jujube-tree girdling for many years on the main nutrients (soluble solid, protein, total acid, vitamin C, total sugar, fat and so on), physical quality attributes (single-fruit weight, hardness and geometric-mean diameter) of jujube fruit and tree health. Through the detection of the nutrients of jujube fruit, it was found that the long-term girdling of jujube trees significantly reduced the main nutrients of jujube fruit. The total sugar and reducing sugar content of jujube-fruit samples from the trees without girdling were 134.23% and 109.62% of the corresponding indexes of jujube-fruit samples from girdled ones, respectively; furthermore, the sugar–acid ratio of jujube samples from the girdled trees was 149.95, while the sugar–acid ratio of fruit from non-girdled trees reached 183.78. Moreover, the protein content of fruit from non-girdled trees was 5.91% higher than that of fruit from girdled ones. In addition, the single-fruit weight, hardness and geometric-average diameter of jujube from non-girdled jujube trees were 20.25%, 13.31% and 20.21% higher than those of jujube from girdled ones, respectively. Moreover, microstructural analysis of the callus and the newly formed phloem showed that the adverse effects of girdling on the phloem function were persistent and permanent. It was also found that jujube-tree girdling can also cause damage, to a certain extent, to the xylem around the girdling wound. The above research results further explained the internal cause of jujube-tree death caused by long-term continuously jujube-tree girdling. The results of this study can provide some scientific basis for the effective application of fruit-tree-girdling technology in fruit production.
Target Enrichment Approaches for Next-Generation Sequencing Applications in Oncology
Authors: Singh Rajesh R.
Published: June 2022

Screening for genomic sequence variants in genes of predictive and prognostic significance is an integral part of precision medicine. Next-generation sequencing (NGS) technologies are progressively becoming platforms of choice to facilitate this, owing to their massively parallel sequencing capability, which can be used to simultaneously screen multiple markers in multiple samples for a variety of variants (single nucleotide and multi nucleotide variants, insertions and deletions, gene copy number variations, and fusions). A crucial step in the workflow of targeted NGS is the enrichment of the genomic regions of interest to be sequenced, against the whole genomic background. This ensures that the NGS effort is focused to predominantly screen target regions of interest with minimal off-target sequencing, making it more accurate and economical. Polymerase chain reaction-based (PCR, or amplicon-based) and hybridization capture-based methodologies are the two prominent approaches employed for target enrichment. This review summarizes the basic principles of target enrichment utilized by these methods, their multiple variations that have evolved over time, automation approaches, overall comparison of their advantages and drawbacks, and commercially available choices for these methodologies.
Intelligent Fault Diagnosis of Industrial Robot Based on Multiclass Mahalanobis-Taguchi System for Imbalanced Data
Authors: Sun Yue , Xu Aidong , Wang Kai , Zhou Xiufang , Guo Haifeng , Han Xiaojia
Published: June 2022

One of the biggest challenges for the fault diagnosis research of industrial robots is that the normal data is far more than the fault data; that is, the data is imbalanced. The traditional diagnosis approaches of industrial robots are more biased toward the majority categories, which makes the diagnosis accuracy of the minority categories decrease. To solve the imbalanced problem, the traditional algorithm is improved by using cost-sensitive learning, single-class learning and other approaches. However, these algorithms also have a series of problems. For instance, it is difficult to estimate the true misclassification cost, overfitting, and long computation time. Therefore, a fault diagnosis approach for industrial robots, based on the Multiclass Mahalanobis-Taguchi system (MMTS), is proposed in this article. It can be classified the categories by measuring the deviation degree from the sample to the reference space, which is more suitable for classifying imbalanced data. The accuracy, G-mean and F-measure are used to verify the effectiveness of the proposed approach on an industrial robot platform. The experimental results show that the proposed approach’s accuracy, F-measure and G-mean improves by an average of 20.74%, 12.85% and 21.68%, compared with the other five traditional approaches when the imbalance ratio is 9. With the increase in the imbalance ratio, the proposed approach has better stability than the traditional algorithms.
Quantitative Evaluation and Obstacle Factor Diagnosis of Agricultural Drought Disaster Risk Using Connection Number and Information Entropy
Authors: Cui Yi , Jin Juliang , Bai Xia , Ning Shaowei , Zhang Libing , Wu Chengguo , Zhang Yuliang
Published: June 2022

To promote the application of entropy concepts in uncertainty analysis of water resources complex system, a quantitative evaluation and obstacle factor diagnosis model of agricultural drought disaster risk was proposed using connection number and information entropy. The results applied to Suzhou City showed that the agricultural drought disaster risks in Suzhou during 2007–2017 were all in middle-risk status, while it presented a decreasing trend from 2010. The information entropy values of the difference degree item bI were markedly lower than those of the difference degree b, indicating that bI provided more information in the evaluation process. Furthermore, the status of drought damage sensitivity and drought hazard were improved significantly. Nevertheless, high exposure to drought and weak drought resistance capacity seriously impeded the reduction of risk. Thus, the key to decreasing risk was to maintain the level of damage sensitivity, while the difficulties were to reduce exposure and enhance resistance. In addition, the percentage of the agricultural population, population density, and percentage of effective irrigation area were the main obstacle factors of risk and also the key points of risk control in Suzhou. In short, the results suggest that the evaluation and diagnosis method is effective and conducive to regional drought disaster risk management.
EGFAFS: A Novel Feature Selection Algorithm Based on Explosion Gravitation Field Algorithm
Authors: Huang Lan , Hu Xuemei , Wang Yan , Fu Yuan
Published: June 2022

Feature selection (FS) is a vital step in data mining and machine learning, especially for analyzing the data in high-dimensional feature space. Gene expression data usually consist of a few samples characterized by high-dimensional feature space. As a result, they are not suitable to be processed by simple methods, such as the filter-based method. In this study, we propose a novel feature selection algorithm based on the Explosion Gravitation Field Algorithm, called EGFAFS. To reduce the dimensions of the feature space to acceptable dimensions, we constructed a recommended feature pool by a series of Random Forests based on the Gini index. Furthermore, by paying more attention to the features in the recommended feature pool, we can find the best subset more efficiently. To verify the performance of EGFAFS for FS, we tested EGFAFS on eight gene expression datasets compared with four heuristic-based FS methods (GA, PSO, SA, and DE) and four other FS methods (Boruta, HSICLasso, DNN-FS, and EGSG). The results show that EGFAFS has better performance for FS on gene expression data in terms of evaluation metrics, having more than the other eight FS algorithms. The genes selected by EGFAGS play an essential role in the differential co-expression network and some biological functions further demonstrate the success of EGFAFS for solving FS problems on gene expression data.
FPGA-Based Real-Time Simulation of Dual-Port Submodule MMC–HVDC System
Authors: Jin Zhao , Wu Yanjie , Wang Shuyuan , Zhang Bingda
Published: June 2022

Aiming at the problems of the high switch numbers, complex working mechanisms, and complicated real-time simulation of modular multilevel converters (MMCs) composed of dual-port submodules, in this study, we designed a unified equivalent model of the multiple submodule network by analyzing the combination of parallel submodules in the bridge arm. The proposed model decouples the submodules that do not affect each other in the subnetwork calculation process, thereby reducing the number of prestored parameters in the subnetwork simulation. In the Xilinx Virtex-7 FPGA VC709 (Xilinx Corporation, San Jose, CA, USA) development board, we replaced the inline computation combined with the prestorage of parameters with the proposed equivalent model to optimize the execution unit structure and redesigned the FPGA-Based Real-Time Digital Solver (FRTDS). Taking the P-FBSM-based MMC–HVDC system as the simulation object, we performed a real-time simulation with a step size of 10 μs, which verified the effectiveness of the proposed model and the improvement in the hardware. We compared the results with the offline MATLAB/Simulink simulation results to verify the accuracy of the simulation.
Transient Thermo-Fluid Analysis of Free Falling CuCl and AgCl Droplets with Liquid-to-Solid Phase Change
Authors: Jianu Ofelia A. , Rajasekaran Bharanidharan
Published: June 2022

Hydrogen extraction from nature is a time-consuming and energy-intensive procedure. Most of the current methods of extracting H2 are not eco-friendly, and the thermochemical copper-chlorine (Cu-Cl) cycle is a promising alternative since the ingredients are continuously recycled within the cycle without discharging pollutants into the atmosphere. In this study, the heat recovered from molten cuprous chloride (CuCl) salt produced in one of the reactors and quenched in a water bath is analyzed numerically to determine the amount of thermal energy that can be recovered and improve the efficiency of the Cu-Cl cycle. The quenching cell is simulated in an inert atmosphere since CuCl is highly reactive in the presence of oxygen. The interactions of various diameters of CuCl droplets within nitrogen (N2) are numerically modeled in COMSOL Multiphysics. Silver chloride (AgCl) is also used in this study to validate the phase-change process. It was discovered in this study that during the free fall, the outer surface of the molten droplets solidifies, and the phase change of droplets slowly propagates radially inwards, which slows down the energy dissipation. It was also determined that the average internal temperature of the droplet does not change substantially with droplet diameter or quenching height. Based on this study, the net energy recovered after quenching was calculated to be around 23 kJ during 1 kg of H2 production.
Electric Drive Solution for Low-Floor City Transport Trams
Authors: Chudzikiewicz Andrzej , Maciejewski Igor , Krzyżyński Tomasz , Krzyszkowski Andrzej , Stelmach Anna
Published: June 2022

The urban transport system based on trams as the basic means of transport is one of the oldest systems of human transport in urban agglomerations. A tram is a more efficient, cheaper-to-operate, and greener means of transport compared to a bus. Striving to enable the use of this means of transport by elderly and disabled people, constructors and manufacturers of tram vehicles began to consider the requirements of the ordering parties—organizers of municipal public transport—in their solutions. The basic condition for disabled and elderly people to use tram transport is the possibility of safe and efficient entry and exit from the vehicle at tram stops. The fulfillment of this condition is possible only in the case of tram vehicles with a low 100% floor, and this, in turn, requires the replacement of trolleys with traditional wheelsets, that is, trolleys with independently rotating wheels, in the construction of the running gear. A wheelset with independently rotating wheels (IRW) does not have self-centering properties, and, thus, problems may arise with excessive wear of wheel and rail profiles and with continuous contact of the wheel flange with the rail, which may, consequently, lead to derailment. Driving a vehicle on the track in this case is governed by different laws. To prevent such phenomena, it is required to use the wheel drive control system, which allows for the stabilization of the vehicle movement on the track. Both the introduction of independently rotating wheels in the construction of the bogie and the drive connected to the wheel control system requires research and analysis to confirm the correctness of the assumptions made. The innovative solution of the control system in the case of a tram vehicle was patented and then the patent was implemented to produce a low-floor tram with 100% low floor by a Polish tram manufacturer. This article presents the results of the work carried out on the adoption of the concept of a running gear and drive solution for a low-floor tram vehicle with independently rotating wheels and the results of simulation analysis of the drive control of such a system, using mathematical models of the mechanical system (running gear) and the electrical system (motor drive control system).
Self-Supporting NiFe Layered Double Hydroxide “Nanoflower” Cluster Anode Electrode for an Efficient Alkaline Anion Exchange Membrane Water Electrolyzer
Authors: Guo Dandan , Chi Jun , Yu Hongmei , Jiang Guang , Shao Zhigang
Published: June 2022

The development of an efficient and durable oxygen evolution reaction (OER) electrode is needed to solve the bottleneck in the application of an anion exchange membrane water electrolyzer (AEMWE). In this work, the self-supporting NiFe layered double hydroxides (NiFe LDHs) “nanoflower” cluster OER electrode directly grown on the surface of nickel fiber felt (Ni fiber) was synthesized by a one-step impregnation at ambient pressure and temperature. The self-supporting NiFe LDHs/Ni fiber electrode showed excellent activity and stability in a three-electrode system and as the anode of AEMWE. In a three-electrode system, the NiFe LDHs/Ni fiber electrode showed excellent OER performance with an overpotential of 208 mV at a current density of 10 mA cm−2 in 1 M KOH. The NiFe LDHs/Ni fiber electrode was used as the anode of the AEMWE, showing high cell performance with a current density of 0.5 A cm−2 at 1.68 V and a stability test for 200 h in 1 M KOH at 70 °C. The electrocatalytic performance of NiFe LDHs/Ni fiber electrode is due to the special morphological structure of “nanoflower” cluster petals stretching outward to produce the “tip effect,” which is beneficial for the exposure of active sites at the edge and mass transfer under high current density. The experimental results show that the NiFe LDHs/Ni fiber electrode synthesized by the one-step impregnation method has the advantages of good activity and low cost, and it is promising for industrial application.
Power Converter Topologies for Grid-Tied Solar Photovoltaic (PV) Powered Electric Vehicles (EVs)—A Comprehensive Review
Authors: Nasr Esfahani Fatemeh , Darwish Ahmed , Williams Barry W.
Published: June 2022

The transport sector generates a considerable amount of greenhouse gas (GHG) emissions worldwide, especially road transport, which accounts for 95% of the total GHGs. It is commonly known that Electric vehicles (EVs) can significantly reduce GHG emissions. However, with a fossil-fuel-based power generation system, EVs can produce more GHGs and therefore cannot be regarded as purely environmentally friendly. As a result, renewable energy sources (RES) such as photovoltaic (PV) can be integrated into the EV charging infrastructure to improve the sustainability of the transportation system. This paper reviews the state-of-the-art literature on power electronics converter systems, which interface with the utility grid, PV systems, and EVs. Comparisons are made in terms of their topologies, isolation, power and voltage ranges, efficiency, and bi-directional power capability for V2G operation. Specific attention is devoted to bidirectional isolated and non-isolated EV-interfaced converters in non-integrated architectures. A brief description of EV charger types, their power levels, and standards is provided. It is anticipated that the studies and comparisons in this paper would be advantageous as an all-in-one source of information for researchers seeking information related to EV charging infrastructures.
Mine Field Preparation and Coal Mining in Western Donbas: Energy Security of Ukraine—A Case Study
Authors: Haidai Oleksandr , Ruskykh Vladyslav , Ulanova Nataliia , Prykhodko Vira , Cabana Edgar Cáceres , Dychkovskyi Roman , Howaniec Natalia , Smolinski Adam
Published: June 2022

Ukrainian electricity production sector is in a critical situation. It is necessary to increase the coal production to ensure the continuity of electricity production. In this paper, an analysis of coal mining in western Donbas, Ukraine, was presented. The crucial thing is to increase coal production in this region. The new calculation schemes were proposed to increase coal extraction in Donbas mines. The coal pillar parameters, which are necessary for the effective control of the rock pressure caused by the stooping, were substantiated. The obtained parameters for the coal pillars will allow extracting more coal, safely and quickly dismantling the mechanized complex and effectively protecting the ongoing mine work. The case study helps determine the coal losses because of pillar leaving and to improve the stability of the main haulage roads located in the zone of the stooping effect as well as to achieve additional coal production in the mine. Such a technological solution allows to extract the coal more fully at the final parts of the extractive columns and to obtain an additional economic effect. The increase in coal production in coal mines of western Donbas will increase the energy security of all Ukraine.
Sustainable Rural Electrification: Harnessing a Cosmolocal Wind
Authors: Troullaki Katerina , Rozakis Stelios , Latoufis Kostas , Giotitsas Chris , Priavolou Christina , Freire Fausto
Published: June 2022

In this article, we explore the sustainability potential of an alternative commons-based mode of production called cosmolocalism. Cosmolocal production combines global knowledge production with local physical production. Such a production mode has been applied across the globe for locally manufacturing small wind turbines (SWTs) for rural electrification. We assess the sustainability of such cosmolocal SWTs in a case study of electrifying a rural community in Ethiopia. In this context, the life cycles of five SWT alternatives have been compared, ranging from conventional industrially produced turbines to open-source locally manufactured and maintained ones. Our case study indicates that the local manufacturing and maintenance of SWTs offer significant advantages and may redeem small wind turbines as a sustainable component for rural electrification. Specifically, the fully cosmolocal alternative (A1) performs better than any other alternative in technical, environmental, and social criteria, while it is close to the best-performing alternative with regard to economic objectives. For this solution to be implemented, the institutional burden cannot be neglected, but can rather be considered a sine qua non condition for locally manufactured and maintained SWTs. A set of generic institutional interventions to create favourable conditions for cosmolocal production is proposed, which needs to be elaborated in a context-specific manner.
Storage and Disposal Options for Nuclear Waste
Authors: Kiegiel Katarzyna
Published: June 2022

Nuclear technology has multiple applications that are fundamental to our daily life [...]
Computational Investigation on the Performance Increase of a Small Industrial Diesel Engine Regarding the Effects of Compression Ratio, Piston Bowl Shape and Injection Strategy
Authors: Hatz Raphael , Lukas Alexander , Zepf Andreas , Jaensch Malte
Published: June 2022

This paper describes the simulative approach to calibrate an already extremely highly turbocharged industrial diesel engine for higher low-speed torque. The engine, which is already operating at its cylinder-pressure maximum, is to achieve close to 30 bar effective mean pressure through suitable calibration between the compression ratio, piston-bowl shape and injection strategy. The basic idea of the study is to lower the compression ratio for even higher injection masses and boost pressures, with the resulting disadvantages in the area of emissions and fuel consumption being partially compensated for by optimizations in the areas of piston shape and injection strategy. The simulations primarily involve the use of the 3D CFD software Converge CFD for in-cylinder calibration and a fully predictive 1D full-engine model in GT Suite. The simulations are based on a two-stage turbocharged 1950 cc four-cylinder industrial diesel engine, which is used for validation of the initial simulation. With the maximum increase in fuel mass and boost pressure, the effective mean pressure could be increased up to 28 bar, while specific consumption increased only slightly. Depending on the geometry, NOx or CO and UHC emissions could be reduced.
Diel Vertical Habitat Use Observations of a Scalloped Hammerhead and a Bigeye Thresher in the Northern Gulf of Mexico
Authors: Anderson Taylor , Meese Emily N. , Drymon James Marcus , Stunz Gregory W. , Falterman Brett , Menjivar Elias , Wells R. J. David
Published: June 2022

Understanding habitat use of elasmobranchs in pelagic environments is complicated due to the mobility of these large animals and their ability to move great distances in a three-dimensional environment. The Gulf of Mexico is a region where many highly migratory pelagic shark species occur, while in close proximity to coastal, anthropogenic activity including recreational and commercial fisheries. This study provides summary information on the vertical habitat use for a single male scalloped hammerhead and a single male bigeye thresher that were each caught and tagged with an archiving satellite tag. The scalloped hammerhead occupied shallow depths (<100 m) over the continental shelf during the 90 d deployment. The bigeye thresher exhibited strong patterns of diel vertical migrations by occupying depths below the thermocline (>350 m) during the day, then occupying shallower depths (50–100 m) during the night. By providing summary information, this note urges future research to provide scientific information on pelagic, highly migratory species for management efforts in the Gulf of Mexico region.
Effect of Siberian Ginseng Water Extract as a Dietary Additive on Growth Performance, Blood Biochemical Indexes, Lipid Metabolism, and Expression of PPARs Pathway-Related Genes in Genetically Improved Farmed Tilapia (Oreochromis niloticus)
Authors: Li Mingxiao , Qiang Jun , Zhu Xiaowen , Bao Jingwen , Tao Yifan , Zhu Haojun
Published: June 2022

Overnutrition in high-density aquaculture can negatively affect the health of farmed fish. The Chinese herbal medicine Siberian ginseng (Acanthopanax senticosus, AS) can promote animal growth and immunity, and regulate lipid metabolism. Therefore, we conducted an 8-week experiment, in which Oreochromis niloticus was fed with a diet supplemented with different concentrations of AS water extract (ASW) (0‰, 0.1‰, 0.2‰, 0.4‰, 0.8‰, and 1.6‰). The ASW improved the growth performance and increased the specific growth rate (SGR). Linear regression analysis based on the SGR estimated that the optimal ASW amount was 0.74‰. Dietary supplementation with 0.4–0.8‰ ASW reduced the triglyceride and total cholesterol levels in the serum and liver, and regulated lipid transport by increasing the high-density lipoprotein cholesterol concentration and lowering the low-density lipoprotein cholesterol concentration. Dietary supplementation with ASW increased the activities of superoxide dismutase and catalase in the liver, thereby improving the antioxidant capacity. Moreover, ASW modulated the transcription of genes in the peroxisome proliferator-activated receptor signaling pathway in the liver (upregulation of PPARα, APOA1b, and FABP10a and downregulation of PPARγ), thereby regulating fatty acid synthesis and metabolism and slowing fat deposition. These results showed that 0.4–0.8‰ ASW can slow fat deposition and protected the liver from cell damage and abnormal lipid metabolism.
Fractional-Order Interval Observer for Multiagent Nonlinear Systems
Authors: Zhang Haoran , Huang Jun , He Siyuan
Published: June 2022

A framework of distributed interval observers is introduced for fractional-order multiagent systems in the presence of nonlinearity. First, a frame was designed to construct the upper and lower bounds of the system state. By using monotone system theory, the positivity of the error dynamics could be ensured, which implies that the bounds could trap the original state. Second, a sufficient condition was applied to guarantee the boundedness of distributed interval observers. Then, an extension of Lyapunov function in the fractional calculus field was the basis of the sufficient condition. An algorithm associated with the procedure of the observer design is also provided. Lastly, a numerical simulation is used to demonstrate the effectiveness of the distributed interval observer.
Diagnostic Pitfall in Atypical Febrile Presentation in a Patient with a Pregnancy-Specific Dermatosis—Case Report and Literature Review
Authors: Mehedintu Claudia , Isopescu Florin , Ionescu Oana-Maria , Petca Aida , Bratila Elvira , Cirstoiu Monica Mihaela , Carp-Veliscu Andreea , Frincu Francesca
Published: June 2022

Pruritic urticarial papules and plaques of pregnancy (PUPPP) usually occurs in the third trimester of pregnancy in primiparous women. It is a self-limiting inflammatory disorder with a still unknown pathogenic mechanism. The abdominal wall overdistension, with a subsequent inflammatory response due to damage to the connective tissue, represents a pathogenesis explanation. Clinical features involve intensely pruritic urticarial rash with edematous, erythematous papules and plaques. The clinical picture and dermal biopsy establish the diagnosis. Topical corticosteroids and oral antihistamines are usually sufficient, but sometimes systemic corticosteroids are necessary. Maternal and fetal prognosis is excellent, and the lesions resolve after birth with no scarring or pigmentary change. We present a case of a 36-year-old patient with a 32-week pregnancy who was admitted with a generalized pruritic rash accompanied by fever. The final diagnosis was decided after multiple pathology exclusions. Treatment consisted of systemic corticoid therapy. The patient gave birth by cesarean section to a healthy newborn without dermatological lesions or other conditions. Adding more PUPPP cases to the literature portfolio will bring more awareness to this under-recognized and under-reported skin disorder. We trust this case will encourage other physicians to publish more cases of pregnancy-specific dermatoses.
Practical Applicability of the ISARIC-4C Score on Severity and Mortality due to SARS-CoV-2 Infection in Patients with Type 2 Diabetes
Authors: Albai Oana , Frandes Mirela , Sima Alexandra , Timar Bogdan , Vlad Adrian , Timar Romulus
Published: June 2022

Background and objectives. There is a bidirectional relationship between SARS-CoV-2 infection and diabetes mellitus (DM), as people with DM are more vulnerable, and SARS-CoV-2 infections worsen the prognosis in these patients. The main purpose of the study was to evaluate the application validity of the ISARIC-4C score in patients confirmed with SARS-CoV-2 infection. Materials and Methods. The study included 159 patients previously known to have type 2 DM and confirmed positive for SARS-CoV-2 infection. We analyzed the concordance between the clinical evaluation of the patients and the ISARIC-4C score. Results. The mortality rate in hospitalized patients was 25.15%. The mortality risk was higher for ISARIC-4C values >14 than in the opposite group (63.93% vs. 31.24%; p < 0.001). The area under the curve (AUC) of the mortality score was 0.875 (95% CI: 0.820–0.930; p < 0.001), correctly classifying 77.36% of the cohort. A cut-off value of >14 had a sensitivity of 87.80% (95% CI: 87.66–87.93), specificity 73.72% (95% CI: 73.48–73.96), positive predictive value 53.73% (95% CI: 53.41–54.04), and negative predictive value 94.56% (95% CI: 94.5–94.62). The Cox regression model showed that the length of hospitalization (LH) was significantly influenced by body mass index, lung impairment, and aspartate aminotransferase, increasing the hazards, while lower HbA1c and lower SatO2 significantly decreased the hazards. Conclusions. ISARIC-4C score estimates the likelihood of clinical deterioration and the mortality risk in patients hospitalized with type 2 DM and positive for SARS-CoV-2, being useful in assessing the prognosis from the onset, as well as in developing therapeutic strategies.
Changes in Serum Creatinine Levels Can Help Distinguish Hypovolemic from Euvolemic Hyponatremia
Authors: Ruiz-Sánchez Jorge Gabriel , Cuesta Martín , Gómez-Hoyos Emilia , Cárdenas-Salas Jersy , Rubio-Herrera Miguel Ángel , Martínez-González Estefanía , De Miguel Novoa Paz , Ternero-Vega Jara Eloisa , Calle-Pascual Alfonso Luis , Runkle Isabelle
Published: June 2022

Background and Objectives: Differentiating between hypovolemic (HH) and euvolemic hyponatremia (EH) is crucial for correct diagnosis and therapy, but can be a challenge. We aim to ascertain whether changes in serum creatinine (SC) can be helpful in distinguishing HH from EH. Materials and Methods: Retrospective analysis of patients followed in a monographic hyponatremia outpatient clinic of a tertiary hospital during 1 January 2014–30 November 2019. SC changes during HH and EH from eunatremia were studied. The diagnostic accuracy of the SC change from eunatremia to hyponatremia (∆SC) was analyzed. Results: A total of 122 hyponatremic patients, median age 79 years (70–85), 46.7% women. In total, 70/122 patients had EH, 52/122 HH. During hyponatremia, median SC levels increased in the HH group: +0.18 mg/dL [0.09–0.39, p < 0.001], but decreased in the EH group: −0.07 mg/dL (−0.15–0.02, p < 0.001), as compared to SC in eunatremia. HH subjects presented a higher rate of a positive ∆SC than EH (90.4% vs. 25.7%, p < 0.001). EH subjects presented a higher rate of a negative/null ∆SC than HH (74.3% vs. 9.6%, p < 0.001). ROC curve analysis found an AUC of 0.908 (95%CI: 0.853 to 0.962, p < 0.001) for ∆SC%. A ∆SC% ≥ 10% had an OR of 29.0 (95%CI: 10.3 to 81.7, p < 0.001) for HH. A ∆SC% ≤ 3% had an OR of 68.3 (95%CI: 13.0 to 262.2, p < 0.001) for EH. Conclusions: The assessment of SC changes from eunatremia to hyponatremia can be useful in distinguishing between HH and EH.
(Low) Energy Availability and Its Association with Injury Occurrence in Competitive Dance: Cross-Sectional Analysis in Female Dancers
Authors: Prus Dasa , Mijatovic Dragan , Hadzic Vedran , Ostojic Daria , Versic Sime , Zenic Natasa , Jezdimirovic Tatjana , Drid Patrik , Zaletel Petra
Published: June 2022

Background and objectives: The risk of low energy availability is related to various health problems in sports. This cross-sectional study aimed to identify a possible association between various dance factors, anthropometrics/body build, and energy availability with injury occurrence in contemporary dancers. Materials and Methods: The participants were 50 female competitive dancers (19.8 ± 4.1 years of age). The independent variables included age, dance factors (amount of training and competitions per week–exposure time, experience in dance), anthropometrics/body composition (body height, mass, BMI, body fat percentage (BF%), and fat-free mass (FFM)), and energy availability score (EAS; evaluated by accelerometer-based measurement of energy expenditure and Dance Energy Availability Questionnaires). The dependent variables were the occurrence of (i) soft-tissue injuries and (ii) bone injuries. The measurements were obtained by experienced technicians during the pre-competition period for each specific dance discipline. Univariate and multivariate logistic regressions were calculated to identify the associations between independent variables and injury prevalence. Results: The results showed that EAS (OR = 0.81, 95% CI:0.65–0.91), age (OR = 1.65, 95% CI: 1.1–2.46), higher BF% (OR = 1.23, 95% CI: 1.04–1.46) and BMI (OR = 1.61, 95% CI: 1.05–2.47) were correlated with soft-tissue injuries. Dancers who suffered from bone injuries reported higher exposure time (OR = 1.21, 95% CI: 1.05–1.37) and had lower values of FFM (OR = 0.73, 95% CI: 0.56–0.98). Multivariate regression analyses evidenced a higher likelihood of soft-tissue injuries in older dancers (OR = 1.75, 95% CI: 1.21–2.95) and the ones who had lower EAS (OR = 0.84, 95% CI: 0.71–0.95) while the exposure time was associated with a higher likelihood of bone injuries (OR = 1.21, 95% CI: 1.05–1.39). Conclusions: In order to decrease the injury prevalence among dancers, special attention should be paid to maintaining adequate nutrition that will provide optimal available energy for the demands of training and performing. Additionally, the control of training volume should be considered in order to reduce traumatic bone injuries.
Should We ‘Eat a Rainbow’? An Umbrella Review of the Health Effects of Colorful Bioactive Pigments in Fruits and Vegetables
Authors: Blumfield Michelle , Mayr Hannah , De Vlieger Nienke , Abbott Kylie , Starck Carlene , Fayet-Moore Flavia , Marshall Skye
Published: June 2022

Health promotion campaigns have advocated for individuals to ‘eat a rainbow’ of fruits and vegetables (FV). However, the literature has only focused on individual color pigments or individual health outcomes. This umbrella review synthesized the evidence on the health effects of a variety of color-associated bioactive pigments found in FV (carotenoids, flavonoids, betalains and chlorophylls), compared to placebo or low intakes. A systematic search of PubMed, EMBASE, CINAHL and CENTRAL was conducted on 20 October 2021, without date limits. Meta-analyzed outcomes were evaluated for certainty via the GRADE system. Risk of bias was assessed using the Centre for Evidence-Based Medicine critical appraisal tools. A total of 86 studies were included, 449 meta-analyzed health outcomes, and data from over 37 million participants were identified. A total of 42% of health outcomes were improved by color-associated pigments (91% GRADE rating very low to low). Unique health effects were identified: n = 6 red, n = 10 orange, n = 3 yellow, n = 6 pale yellow, n = 3 white, n = 8 purple/blue and n = 1 green. Health outcomes associated with multiple color pigments were body weight, lipid profile, inflammation, cardiovascular disease, mortality, type 2 diabetes and cancer. Findings show that color-associated FV variety may confer additional benefits to population health beyond total FV intake.
Antidiabetic, Antiglycation, and Antioxidant Activities of Ethanolic Seed Extract of Passiflora edulis and Piceatannol In Vitro
Authors: dos Santos Flávia A. R. , Xavier Jadriane A. , da Silva Felipe C. , Merlin J. P. Jose , Goulart Marília O. F. , Rupasinghe H. P. Vasantha
Published: June 2022

The objective of this work was to investigate the antidiabetic, antiglycation, and antioxidant potentials of ethanolic extract of seeds of Brazilian Passiflora edulis fruits (PESE), a major by-product of the juice industry, and piceatannol (PIC), one of the main phytochemicals of PESE. PESE, PIC, and acarbose (ACB) exhibited IC50 for alpha-amylase, 32.1 ± 2.7, 85.4 ± 0.7, and 0.4 ± 0.1 µg/mL, respectively, and IC50 for alpha-glucosidase, 76.2 ± 1.9, 20.4 ± 7.6, and 252 ± 4.5 µg/mL, respectively. The IC50 of PESE, PIC, and sitagliptin (STG) for dipeptidyl-peptidase-4 (DPP-4) was 71.1 ± 2.6, 1137 ± 120, and 0.005 ± 0.001 µg/mL, respectively. PESE and PIC inhibited the formation of advanced glycation end-products (AGE) with IC50 of 366 ± 1.9 and 360 ± 9.1 µg/mL for the initial stage and 51.5 ± 1.4 and 67.4 ± 4.6 µg/mL for the intermediate stage of glycation, respectively. Additionally, PESE and PIC inhibited the formation of β-amyloid fibrils in vitro up to 100%. IC50 values for 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•) scavenging activity of PESE and PIC were 20.4 ± 2.1, and 6.3 ± 1.3 µg/mL, respectively. IC50 values for scavenging hypochlorous acid (HOCl) were similar in PESE, PIC, and quercetin (QCT) with values of 1.7 ± 0.3, 1.2 ± 0.5, and 1.9 ± 0.3 µg/mL, respectively. PESE had no cytotoxicity to the human normal bronchial epithelial (BEAS-2B), and alpha mouse liver (AML-12) cells up to 100 and 50 µg/mL, respectively. However, 10 µg/mL of the extract was cytotoxic to non-malignant breast epithelial cells (MCF-10A). PESE and PIC were found to be capable of protecting cultured human cells from the oxidative stress caused by the carcinogen NNKOAc at 100 µM. The in vitro evidence of the inhibition of alpha-amylase, alpha-glucosidase, and DPP-4 enzymes as well as antioxidant and antiglycation activities, warrants further investigation of the antidiabetic potential of P. edulis seeds and PIC.
Buckwheat Hull-Enriched Pasta: Physicochemical and Sensory Properties
Authors: Sujka Katarzyna , Cacak-Pietrzak Grażyna , Sułek Alicja , Murgrabia Karolina , Dziki Dariusz
Published: June 2022

This work aimed to evaluate the effect of partial replacement of semolina with 0, 1, 5, 10, 15, and 20% of ground buckwheat hull (BH) on the chemical composition, antioxidant properties, color, cooking characteristics, and sensory properties of wheat pasta. Pasta samples were prepared by dough lamination (tagliatelle shape) and dried at 55 °C until the moisture content was 11–12% (wet basis). Analyses of samples showed that the addition of BH caused an increase in fiber content in pasta from 4.31% (control pasta) to 14.15% (pasta with 20% of BH). Moreover, the brightness and yellowness of BH-enriched products were significantly decreased compared to the control sample, and the total color difference ranged from 23.84 (pasta with 1% of BH) to 32.56 (pasta with 15% BH). In addition, a decrease in optimal cooking time, as well as an increased weight index and cooking loss, was observed in BH-enriched pasta samples. Furthermore, BH-enriched cooked pasta had significantly higher total phenolic content and antioxidant activity but an unpleasant smell and taste, especially if the level of BH was higher than 10%.
Continued Structural Exploration of Sulfocoumarin as Selective Inhibitor of Tumor-Associated Human Carbonic Anhydrases IX and XII
Authors: Giovannuzzi Simone , Capasso Clemente , Nocentini Alessio , Supuran Claudiu T.
Published: June 2022

A series of new 3- and 7-substituted sulfocoumarins was obtained by several cyclization reactions and subsequent derivatization for screening as prodrug inhibitors of the human (h) cancer-associated carbonic anhydrases (CAs) IX and XII. All products were ineffective inhibitors against the off-target hCA I and II, whilst hCAs IX and XII were inhibited with inhibition constants (KIs) spanning from low nanomolar to the high micromolar range, according to the sulfocoumarin derivatization pattern. In particular, sulfocoumarin 15 turned out to be the most potent and selective inhibitor herein reported (hCA I and II: KI > 100 µM; hCA IX: KI = 22.9 nM; hCA XII: KI = 19.2 nM). Considering that hCA IX and XII validated anti-tumor targets, such prodrug, isoform-selective inhibitors as the sulfocoumarins reported here may be useful for identifying suitable drug candidates for clinical trials.
Phytochemical Profiling, Anti-Inflammatory, Anti-Oxidant and In-Silico Approach of Cornus macrophylla Bioss (Bark)
Authors: Khan Ali , Pervaiz Aini , Ansari Bushra , Ullah Riaz , Shah Syed Muhammad Mukarram , Khan Haroon , Saeed Jan Muhammad , Hussain Fida , Ijaz Khan Mohammad , Albadrani Ghadeer M. , Altyar Ahmed E. , Abdel-Daim Mohamed M.
Published: June 2022

The objective of the current study was to evaluate the phytochemical and pharmacological potential of the Cornus macrophylla. C. macrophylla belongs to the family Cornaceae. It is locally known as khadang and is used for the treatment of different diseases such as analgesic, tonic, diuretic, malaria, inflammation, allergy, infections, cancer, diabetes, and lipid peroxidative. The crude extract and different fractions of C. macrophyll were evaluated by gas chromatography and mass spectroscopy (GC-MS), which identified the most potent bioactive phytochemicals. The antioxidant ability of C. macrophylla was studied by 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) and 1,1 diphenyl-2-picryl-hydrazyl (DPPH) methods. The crude and subsequent fractions of the C. macrophylla were also tested against anti-inflammatory enzymes using COX-2 (Cyclooxygenase-2) and 5-LOX (5-lipoxygenase) assays. The molecular docking was carried out using molecular operating environment (MOE) software. The GC-MS study of C. macrophylla confirmed forty-eight compounds in ethyl acetate (Et.AC) fraction and revealed that the Et.AC fraction was the most active fraction. The antioxidant ability of the Et.AC fraction showed an IC50 values of 09.54 μg/mL and 7.8 μg/mL against ABTS and DPPH assay respectively. Among all the fractions of C. macrophylla, Et.AC showed excellent activity against COX-2 and 5-LOX enzyme. The observed IC50 values were 93.35 μg/mL against COX-2 and 75.64 μg/mL for 5-LOX respectively. Molecular docking studies supported these in vitro results and confirmed the anti-inflammatory potential of C. macrophylla. C. macrophylla has promising potential as a source for the development of new drugs against inflammation in the future.
Kinetic Study of 17α-Estradiol Mechanism during Rat Sperm Capacitation
Authors: Bosakova Tereza , Tockstein Antonin , Bosakova Zuzana , Komrskova Katerina
Published: June 2022

17α-Estradiol (αE2) is a natural diastereoisomer of 17β-estradiol (E2). It is well known that αE2 can bind to estrogen receptors. However, its biological activity is less than that of E2 and is species and tissue specific. The goal of our study was to propose the mechanism of αE2 hormonal response in rat sperm during their capacitation in vitro and compare it with a previously studied mouse model. Concentration changes in externally added αE2 during capacitation of rat sperm were monitored by the high-performance liquid chromatographic method with tandem mass spectrometric detection (HPLC-MS/MS). The calculated values of relative concentrations Bt were subjected to kinetic analysis. The findings indicated that αE2 in rat sperm did not trigger autocatalytic reaction, in contrast to the mouse sperm, and that the initiation of the hormone penetration through the sperm plasma membrane was substantially faster in rats.
Carbon Quantum Dots from Pomelo Peel as Fluorescence Probes for “Turn-Off–On” High-Sensitivity Detection of Fe3+ and L-Cysteine
Authors: Zhang Dianwei , Zhang Furui , Liao Yonghong , Wang Fenghuan , Liu Huilin
Published: June 2022

This study designed a “turn-off–on” fluorescence analysis method based on carbon quantum dots (CQDs) to detect metal ions and amino acids in real sample systems. CQDs were derived from green pomelo peel via a one-step hydrothermal process. The co-doped CQDs with N and S atoms imparted excellent optical properties (quantum yield = 17.31%). The prepared CQDs could be used as fluorescent “turn-off” probes to detect Fe3+ with a limit of detection of 0.086 µM, a linear detection range of 0.1–160 µM, and recovery of 83.47–106.53% in water samples. The quenched CQD fluorescence could be turned on after adding L-cysteine (L-Cys), which allowed detection of L-Cys with a detection limit of 0.34 µM and linear range of 0.4–85 µM. Recovery of L-Cys in amino acid beverage was 87.08–122.74%. Visual paper-based testing strips and cellulose/CQDs composite hydrogels could be also used to detect Fe3+ and L-Cys.
GC-MS/MS Quantification of EGFR Inhibitors, β-Sitosterol, Betulinic Acid, (+) Eriodictyol, (+) Epipinoresinol, and Secoisolariciresinol, in Crude Extract and Ethyl Acetate Fraction of Thonningia sanguinea
Authors: Elhady Sameh S. , Ibrahim Elsayed A. , Goda Marwa S. , Nafie Mohamed S. , Samir Hanan , Diri Reem M. , Alahdal Abdulrahman M. , Thomford Ama Kyeraa , El Gindy Alaa , Hadad Ghada M. , Badr Jihan M. , Abdelhameed Reda F. A.
Published: June 2022

Medicinal plants are widely used in folk medicine to treat various diseases. Thonningia sanguinea Vahl is widespread in African traditional medicine, and exhibits antioxidant, antibacterial, antiviral, and anticancer activities. T. sanguinea is a source of phytomedicinal agents that have previously been isolated and structurally elucidated. Herein, gas chromatography combined with tandem mass spectrometry (GC-MS/MS) was used to quantify epipinoresinol, β-sitosterol, eriodictyol, betulinic acid, and secoisolariciresinol contents in the methanolic crude extract and its ethyl acetate fraction for the first time. The ethyl acetate fraction was rich in epipinoresinol, eriodictyol, and secoisolariciresinol at concentrations of 2.3, 3.9, and 2.4 mg/g of dry extract, respectively. The binding interactions of these compounds with the epidermal growth factor receptor (EGFR) were computed using a molecular docking study. The results revealed that the highest binding affinities for the EGFR signaling pathway were attributed to eriodictyol and secoisolariciresinol, with good binding energies of −19.93 and −16.63 Kcal/mol, respectively. These compounds formed good interactions with the key amino acid Met 769 as the co-crystallized ligand. So, the ethyl acetate fraction of T. sanguinea is a promising adjuvant therapy in cancer treatments.
Toward Oral Supplementation of Diamine Oxidase for the Treatment of Histamine Intolerance
Authors: Kettner Lucas , Seitl Ines , Fischer Lutz
Published: June 2022

A new diamine oxidase (DAO-1) was discovered recently in the yeast Yarrowia lipolytica PO1f and investigated for its histamine degradation capability under simulated intestinal conditions. DAO-1 was formulated together with catalase as a sucrose-based tablet. The latter (9 × 7 mm; 400 mg) contained 690 nkat of DAO-1 activity, which was obtained from a bioreactor cultivation of a genetically modified Y. lipolytica with optimized downstream processing. The DAO-1 tablet was tested in a histamine bioconversion experiment under simulated intestinal conditions in the presence of food constituents, whereby about 30% of the histamine was degraded in 90 min. This amount might already be sufficient to help people with histamine intolerance. Furthermore, it was found that the stability of DAO-1 in a simulated intestinal fluid is influenced distinctively by the presence of a food matrix, indicating that the amount and type of food consumed affect the oral supplementation with DAO. This study showed for the first time that a microbial DAO could have the potential for the treatment of histamine intolerance by oral supplementation.