bluuebunny/crossref_metadata_2025 huggingface dataset:
Motivation and Productivity of Employees in Higher Education during the First Lockdown
Authors: Rietveld Jacqueline R. , Hiemstra Djoerd , Brouwer Aleid E. , Waalkens Jan
Published: December 2021

In a cross-sectional study among 623 employees of a higher education institution, we examined the relations between perceived competence, autonomy, relatedness, intrinsic motivation, and productivity during the first lockdown in the spring of 2020. The results indicate that, relative to the period before the lockdown, the employees experienced an increase in autonomy and competence, but a decrease in relatedness, intrinsic motivation, and productivity. Structural equation modelling revealed that the decrease in productivity can be explained by a decrease in intrinsic motivation, which in turn can be explained by changes in relatedness, autonomy, and perceived competence. Thus, during the lockdown, both positive and negative motivational consequences of teleworking were observed. However, the ultimate consequence for employees’ productivity was negative. An important difference between this study and previous studies on the topic of teleworking, is that the present examined the motivational process under extreme circumstances in which employees had to switch overnight form onsite to remote working.
Increasing the Efficiency of Aircraft Ground Handling—A Case Study
Authors: Szabo Stanislav , Pilát Marek , Makó Sebastián , Korba Peter , Čičváková Miroslava , Kmec Ľubomír
Published: December 2021

This research was focused on increasing the efficiency of aircraft ground handling at the airport. The main goal of the research was to improve the individual processes that are part of the aircraft ground handling in order to speed up this operation, as well as improve the turnaround time between individual flights to enhance the overall throughput of airport stands. The objective of the research was to measure the times of standard airport processes that are part of the aircraft handling, measure the turnaround time between individual flights at the selected airport and increase the efficiency of each process that was measured. After the measurements had been taken, changes were introduced, and the times were measured again. The changes were mainly focused on the following aspects: the position of ground handling equipment before the arrival of the aircraft, the deployment of staff, and the routes taken by ground handling equipment. The measurements were taken during the summer on a fixed stand, with a standard number of employees and with the same type of aircraft. In total, 78 measurements were taken in which 2340 partial times were measured during the entire course of aircraft ground handling before departure to the next destination. After the changes were implemented, the same measurements were taken again to see if the changes that had been implemented could speed up the overall process of the aircraft ground handling. Subsequently, all data were evaluated using statistical methods. All measurements were done at the Košice airport.
Implementation of Straw Racks in Commercial Pig Housing—Impact on Straw Availability and Pig Behaviour
Authors: Wallgren Torun , Gunnarson Stefan
Published: December 2021

This study investigated if straw racks, which enable larger straw rations, induced a more straw-directed behaviour in pigs, as a mean to facilitate the provision of manipulable material and natural behaviour. It was conducted on a commercial farm (459 pigs, 42 pens, 30–120 kg) where half of the pens received 25 L of straw on the floor (CONTROL) and the other half of the pens received straw in a rack holding 44 L of straw (RACK). The pig behaviour in five randomly assigned pens per treatment were recorded for 24 h, during three periods of production. Pig activity levels, exploratory behaviour, pen utilization and available clean straw were scan sampled. During period 1, no pigs were observed interacting with the straw racks. During this period, CONTROL pigs conducted more straw-directed behaviour and less pen-directed behaviour compared to pigs in the pens with a rack during period 1. The lack of rack interactions may imply an underdeveloped spatial cognition in the pigs. Apart from period 1, there were no significant difference in behaviour between RACK and CONTROL pigs. The racks did not disturb the use of the pen. The absent treatment effect in periods 2 and 3 may reflect that there was a too small difference in straw ration between the treatments. In order to design and implement straw racks that promote straw interaction, future studies should focus on understanding pigs’ spatial cognition.
Analysis of the Content Values of Sweet Maize (Zea mays L. Convar Saccharata Koern) in Precision Farming
Authors: Demeter Cintia , Nagy János , Huzsvai László , Zelenák Annabella , Szabó Atala , Széles Adrienn
Published: December 2021

The global precision farming area is constantly increasing, and precision sweet maize production developed the most. Sweet maize yield is above average in precision farming. Additionally, its role in healthy nutrition is becoming increasingly important due to new hybrids with high carotenoid content. Precision farming techniques are needed to produce healthy food. In particular, nutrient supply and irrigation, sowing, crop management and harvesting need to be carried out with precision techniques. These factors are all prerequisites for effective and healthy growing and processing. The aim was to use the yields of the four sweet maize hybrids grown on the largest area to examine their nutritional values and concentrations (mg kg−1 dry matter) and to analyse their yield per hectare. Concentration is important for the consumer because K, P, Mg, Ca, Fe, Zn, and Na play an important role in metabolism, skin protection, and bone and tooth health. The new results obtained show that the amount of lutein and zeaxanthin per hectare is important for the processing industry, especially for use in food supplements. Their anti-inflammatory effects and their role in disease prevention (cardiovascular diseases, Age-Related Macular Degeneration (AMD)) have been demonstrated. Consumers choose sweet maize mainly on the basis of its palatability, which is why the sugar content of the hybrids was also studied. We assumed that the element concentration in the yield of new hybrids with higher yield per hectare does not decrease with increasing yield. The concentrations of zeaxanthin, β-cryptoxanthin and β-carotene appear in one principal component and they are in close positive correlation with each other. The lutein concentration was independent of the former three compounds. The independence of the lutein concentration means that it is not possible to estimate its amount based on the other three components. For yield per unit area, the correlation is one-dimensional. Yield determines the lutein, zeaxanthin, β-cryptoxanthin and β-carotene concentrations per hectare.
Variation in Nutritional Components in Roots from Ahipa (Pachyrhizus ahipa (Wedd.) Parodi) Accessions and an Interspecific Hybrid (P. ahipa × P. tuberosus (Lam.) Spreng.)
Authors: Leidi Eduardo O. , Ech-Chliah Youssef , Rossini-Oliva Sabina , Sørensen Marten
Published: December 2021

Among the many neglected underutilized species, tuberous Andean root crops such as the ahipas (Pachyrhizus ahipa) constitute a promising alternative for increasing diversity in nutrient sources and food security at a regional level. ahipa × P. tuberosus). A significant objective was to determine protein and free amino acids in the roots to evaluate their food quality as protein supply. The interspecific hybrids have been found to possess the root quality to provide the crop with a higher dry matter content. The high dry matter content of the P. tuberosus Chuin materials is retained in the root quality of the hybrids. Food functional components such as carbohydrates, organic acids, and proteins were determined in several ahipa accessions and a stable (non-segregating) progeny of the interspecific hybrid, X207. The X207 roots showed a significantly higher dry matter content and a lower content in soluble sugars, but no significant differences were found in starch content or organic acids compared to the ahipa accessions. Regarding the root mineral contents, Fe and Mn concentrations in X207 were significantly raised compared to the average of ahipa accessions. Among the ahipa and the hybrid, no prominent differences in protein content or protein amino acids were found, being both partially defective in providing sufficient daily intake of some essential amino acids. Root weight, a central component of root yield, was significantly higher in X207, but thorough field studies are required to substantiate the hybrid’s superior yield performance.
Investigation of Cyprinid Herpesvirus 3 (CyHV-3) Disease Periods and Factors Influencing CyHV-3 Transmission in A Low Stocking Density Infection Trial
Authors: Tolo Isaiah E. , Bajer Przemyslaw G. , Wolf Tiffany M. , Mor Sunil K. , Phelps Nicholas B. D.
Published: December 2021

Cyprinid herpesvirus 3 (CyHV-3) is the etiological agent of koi herpesvirus disease (KHVD) and important pathogen of aquaculture and wild populations of common carp worldwide. Understanding the relative contributions of direct and indirect transmission of CyHV-3 as well as the factors that drive CyHV-3 transmission can clarify the importance of environmental disease vectors and is valuable for informing disease modeling efforts. To study the mechanisms and factors driving CyHV-3 transmission we conducted infection trials that determined the kinetics of KHVD and the contributions of direct and indirect forms of CyHV-3 transmission, as well as the contributions of contact rate, viral load, pathogenicity and contact type. The incubation period of KHVD was 5.88 + 1.75 days and the symptomatic period was 5.31 + 0.87 days. Direct transmission was determined to be the primary mechanism of CyHV-3 transmission (OR = 25.08, 95%CI = 10.73–99.99, p = 4.29 × 10−18) and transmission primarily occurred during the incubation period of KHVD. Direct transmission decreased in the symptomatic period of disease. Transmissibility of CyHV-3 and indirect transmission increased during the symptomatic period of disease, correlating with increased viral loads. Additionally, potential virulence-transmission tradeoffs and disease avoidance behaviors relevant to CyHV-3 transmission were identified.
Influencing Activity of Bats by Dimly Lighting Wind Turbine Surfaces with Ultraviolet Light
Authors: Cryan Paul M. , Gorresen Paulo M. , Straw Bethany R. , Thao Syhoune (Simon) , DeGeorge Elise
Published: December 2021

Wind energy producers need deployable devices for wind turbines that prevent bat fatalities. Based on the speculation that bats approach turbines after visually mistaking them for trees, we tested a potential light-based deterrence method. It is likely that the affected bats see ultraviolet (UV) light at low intensities. Here, we present the results of a multi-month experiment to cast dim, flickering UV light across wind turbine surfaces at night. Our objectives were to refine and test a practical system for dimly UV-illuminating turbines while testing whether the experimental UV treatment influenced the activity of bats, birds, and insects. We mounted upward-facing UV light arrays on turbines and used thermal-imaging cameras to quantify the presence and activity of night-flying animals. The results demonstrated that the turbines can be lit to the highest reaches of the blades with “invisible” UV light, and the animal responses to such experimental treatment can be concurrently monitored. The UV treatment did not significantly change nighttime bat, insect, or bird activity at the wind turbine. Our findings show how observing flying animals with thermal cameras at night can help test emerging technologies intended to variably affect their behaviors around wind turbines.
Long-Term Effects of Biliverdin Reductase a Deficiency in Ugt1−/− Mice: Impact on Redox Status and Metabolism
Authors: Bortolussi Giulia , Shi Xiaoxia , ten Bloemendaal Lysbeth , Banerjee Bhaswati , De Waart Dirk R. , Baj Gabriele , Chen Weiyu , Oude Elferink Ronald P. , Beuers Ulrich , Paulusma Coen C. , Stocker Roland , Muro Andrés F. , Bosma Piter J.
Published: December 2021

Accumulation of neurotoxic bilirubin due to a transient neonatal or persistent inherited deficiency of bilirubin glucuronidation activity can cause irreversible brain damage and death. Strategies to inhibit bilirubin production and prevent neurotoxicity in neonatal and adult settings seem promising. We evaluated the impact of Bvra deficiency in neonatal and aged mice, in a background of unconjugated hyperbilirubinemia, by abolishing bilirubin production. We also investigated the disposal of biliverdin during fetal development. In Ugt1−/− mice, Bvra deficiency appeared sufficient to prevent lethality and to normalize bilirubin level in adults. Although biliverdin accumulated in Bvra-deficient fetuses, both Bvra−/− and Bvra−/−Ugt1−/− pups were healthy and reached adulthood having normal liver, brain, and spleen histology, albeit with increased iron levels in the latter. During aging, both Bvra−/− and Bvra−/−Ugt1−/− mice presented normal levels of relevant hematological and metabolic parameters. Interestingly, the oxidative status in erythrocytes from 9-months-old Bvra−/− and Bvra−/−Ugt1−/− mice was significantly reduced. In addition, triglycerides levels in these 9-months-old Bvra−/− mice were significantly higher than WT controls, while Bvra−/−Ugt1−/− tested normal. The normal parameters observed in Bvra−/−Ugt1−/− mice fed chow diet indicate that Bvra inhibition to treat unconjugated hyperbilirubinemia seems safe and effective.
Glutathione Is Required for the Early Alert Response and Subsequent Acclimation in Cadmium-Exposed Arabidopsis thaliana Plants
Authors: Deckers Jana , Hendrix Sophie , Prinsen Els , Vangronsveld Jaco , Cuypers Ann
Published: December 2021

Pollution by cadmium (Cd) is a worldwide problem, posing risks to human health and impacting crop yield and quality. Cadmium-induced phytotoxicity arises from an imbalance between antioxidants and pro-oxidants in favour of the latter. The Cd-induced depletion of the major antioxidant glutathione (GSH) strongly contributes to this imbalance. Rather than being merely an adverse effect of Cd exposure, the rapid depletion of root GSH levels was proposed to serve as an alert response. This alarm phase is crucial for an optimal stress response, which defines acclimation later on. To obtain a better understanding on the importance of GSH in the course of these responses and how these are defined by the rapid GSH depletion, analyses were performed in the GSH-deficient cadmium-sensitive 2-1 (cad2-1) mutant. Cadmium-induced root and leaf responses related to oxidative challenge, hydrogen peroxide (H2O2), GSH, ethylene, and 1-aminocyclopropane-1-carboxylic acid (ACC) were compared between wild-type (WT) and mutant Arabidopsis thaliana plants. Although the cad2-1 mutant has significantly lower GSH levels, root GSH depletion still occurred, suggesting that the chelating capacity of GSH is prioritised over its antioxidative function. We demonstrated that responses related to GSH metabolism and ACC production were accelerated in mutant roots and that stress persisted due to suboptimal acclimation. In general, the redox imbalance in cad2-1 mutant plants and the lack of proper transient ethylene signalling contributed to this suboptimal acclimation, resulting in a more pronounced Cd effect.
Ambulance Locations in a Tiered Emergency Medical System in a City
Authors: Jankovič Peter , Jánošíková Ľudmila
Published: December 2021

This paper deals with optimizing the location of ambulance stations in a two-tiered emergency medical system in an urban environment. Several variants of station distribution are calculated by different mathematical programming models and are evaluated by a detailed computer simulation model. A new modification of the modular capacitated location model is proposed. Two ways of demand modelling are applied; namely, the aggregation of the ambient population and the aggregation of permanent residents at the street level. A case study of the city of Prešov, Slovakia is used to assess the models. The performance of the current and proposed sets of locations is evaluated using real historical data on ambulance trips. Computer simulation demonstrates that the modular capacitated location model, with the ambient population demand, significantly reduces the average response time to high-priority patients (by 79 s in the city and 62 s in the district) and increases the percentage of high-priority calls responded to within 8 min (by almost 4% in the city and 5% in the district). Our findings show that a significant improvement in the availability of the service can be achieved when ambulances are not accumulated at a few stations but rather spread over the city territory.
Efficient Lattice-Based Cryptosystems with Key Dependent Message Security
Authors: Yang Bo , Huang Ruwei , Zhao Jianan
Published: December 2021

Key-dependent message (KDM) security is of great research significance, to better analyse and solve the potential security problems in complex application scenarios. Most of the current KDM security schemes are based on traditional hard mathematical problems, where the public key and ciphertext are not compact enough, and make the ciphertext size grow linearly with the degree of the challenge functions. To solve the above problems and the inefficient ciphertext operation, the authors propose a compact lattice-based cryptosystem with a variant of the RLWE problem, which applies an invertible technique to obtain the RLWE* problem. It remains hard after the modification from the RLWE problem. Compared with the ACPS scheme, our scheme further expands the set of challenge functions based on the affine function of the secret key, and the size of public key and ciphertext is O˜(n), which is independent of the challenge functions. In addition, this scheme enjoys a high level of efficiency, the cost of encryption and decryption is only ploylog(n) bit operations per message symbol, and we also prove that our scheme is KDM-CPA secure under the RLWE* assumption.
Differential Response of the Proteins Involved in Amino Acid Metabolism in Two Saccharomyces cerevisiae Strains during the Second Fermentation in a Sealed Bottle
Authors: González-Jiménez María del Carmen , Mauricio Juan Carlos , Moreno-García Jaime , Puig-Pujol Anna , Moreno Juan , García-Martínez Teresa
Published: December 2021

The traditional method for sparkling wine making consists of a second fermentation of a base wine followed by ageing in the same bottle that reaches the consumers. Nitrogen metabolism is the second most important process after carbon and takes place during wine fermentation by yeast. Amino acids are the most numerous nitrogen compounds released by this process. They contribute to the organoleptic properties of the wines and, therefore, to their sensory quality. The main objective of this study is to compare the differential proteomic response of amino acid metabolism, specifically their proteins and their interactions in the G1 strain (unconventional yeast) during sparkling wine production versus the conventional P29 strain. One of the new trends in winemaking is the improvement of the organoleptic diversity of wine. We propose the use of unconventional yeast that shows desirable characteristics for the industry. For this purpose, these two yeasts were grown at sealed bottle conditions for the second fermentation (Champenoise method). No differences were obtained in the middle of fermentation between the yeast strains. The number of proteins identified, and the relationships established, were similar, highlighting lysine metabolism. At the end of the second fermentation, the difference between each strain was remarkable. Hardly any proteins were identified in unconventional versus conventional yeast. However, in both strains, the metabolism of sulfur amino acids, methionine, and cysteine obtained a greater number of proteins involved in these processes. The release of these amino acids to the medium would allow the yeast to balance the internal redox potential by reoxidation of NADPH. This study is focused on the search for a more complete knowledge of yeast metabolism, specifically the metabolism of amino acids, which are key compounds during the second fermentation.
A Comparative Study to Evaluate the Effect of Honey and Zinc Oxide Eugenol Dressing for the Treatment of Dry Socket: A Double-Blind Randomized Controlled Trial
Authors: Khan Zafar Ali , Prabhu Namdeo , Ahmed Naseer , Lal Abhishek , Issrani Rakhi , Maqsood Afsheen , Alam Mohammad Khursheed , Alanazi Sarah , Aljohani Fahad Muqbil , Almndel Msleh Naim , Alolait Mshari Ali Abdullah
Published: December 2021

Dry socket is a common postoperative complication secondary to any tooth extraction but is more commonly associated with mandibular third molars. Dry socket has been treated with various treatment modalities with different success rates. This study aimed to evaluate the effect of using honey and zinc oxide eugenol as an intra-socket medicament for the treatment of dry sockets. Ninety patients were divided into three groups as follows: Group 1 “honey”: 30 patients; Group 2 “zinc oxide eugenol”: 30 patients; and Group 3 “control”: 30 patients. Pre-medication and post-medication pain scores at different time intervals were measured on a verbal rating scale. The mean ages of the patients in each of the groups were as follows: Group 1: 44.26 ± 14.14; Group 2: 45.30 ± 18.08; and Group 3: 51.93 ± 9.75. As regards verbal rating scale pain scores, a significant difference was noted in patients that belonged to Groups 1 and 2, with an immediate reduction in post-medication pain scores. However, patients belonging to Group 3 reported pain and discomfort for over a week. The use of honey and zinc oxide eugenol resulted in significant immediate post-medication pain scores in patients as compared to the control group. Therefore, both honey and zinc oxide eugenol can be used as treatment options for dry socket patients.
Burned Area Classification Based on Extreme Learning Machine and Sentinel-2 Images
Authors: Gajardo John , Mora Marco , Valdés-Nicolao Guillermo , Carrasco-Benavides Marcos
Published: December 2021

Sentinel-2 satellite images allow high separability for mapping burned and unburned areas. This problem has been extensively addressed using machine-learning algorithms. However, these need a suitable dataset and entail considerable training time. Recently, extreme learning machines (ELM) have presented high precision in classification and regression problems but with low computational cost. This paper proposes evaluating ELM to map burned areas and compare them with other machine-learning algorithms broadly used. Several indices, metrics and training times were used to assess the performance of the algorithms. Considering the average of datasets, the best performance was obtained by random forest (DICE = 0.93; omission and commission = 0.08) and ELM (DICE = 0.90; omission and commission = 0.07). The training time for the best model was from ELM (1.45 s) and logistic regression (1.85 s). According to results, ELM was the best burned-area classification algorithm, considering precision and training time, evidencing great potential to map burned areas at global scales with medium-high spatial resolution images. This information is essential to fire-risk systems and burned-area records used to design prevention and fire-combat strategies, and it provides valuable knowledge on the effect of fires on the landscape and atmosphere.
Fuzzy Logic in Selection of Maritime Search and Rescue Units
Authors: Malyszko Marzena
Published: December 2021

The article discusses methods of ships assessment when determining their suitability for search and rescue action (SAR) at sea. Selection of the most preferable ships is one of the action planning elements. Due to various construction and equipment the civilian ships can only perform rescue task to a certain degree. According to the Multi-Criteria Decision Analysis (MCDA), many parameters and data have to be compared in order to create a ranking of vessels ordered according to the coordinator’s preferences. When data are missing, incomplete or uncertain, a similar effect can be obtained using fuzzy logic. The author discussed the nature of the criteria, evaluation methods and presented a simulation of a ship study using fuzzy logic. The author developed fuzzy rules and presented the principle of operation of the controller. The article deals with the main principles of a decision support system (DSS) for the selection of ships in SAR operations.
Specifics of Explosion-Venting Structures Providing Acceptable Indoor Explosion Loads
Authors: Komarov Alexander Andreevich , Korolchenko Dmitry Aleksandrovich , Gromov Nikolay Viktorovich , Korolchenko Anton Dmitrievich
Published: December 2021

This article experimentally and theoretically demonstrates that the presence of blast-relief openings (windows) equipped with explosion-venting structures (EVS) allows explosive pressure to be reduced to a safe level (2–4 kPa). We provide results of model and full-scale experiments aimed at studying the influence of EVS parameters of blast-relief openings in explosion-hazardous buildings on the intensity of explosive loads. It was demonstrated that the maximum explosive-pressure value inside EVS-equipped buildings depends on the EVS start-to-open pressure, the structure’s response rate (lag), and characteristic dimension of the premises. Thus, each particular building requires individual selection of EVS parameters, which provide a safe level of excessive pressure in case of an explosive accident. This aspect, however, prevents the widespread use of EVS at explosion-hazardous sites. This article offers an modest upgrade of the explosion-venting structure that provides an indoor pressure equal to the EVS start-to-open pressure. The suggested innovation excludes the possibility of a significant increase in explosive pressure due to an EVS response delay. The efficiency of the suggested technical upgrade was proven by numerical experiments and indirectly by experimental studies aimed at exploring the physical processes associated with the opening of EVSs after an explosion accident. The use of upgraded EVSs will allow for provision of a known maximum level of the explosion load should an explosion event occur in an EVS-equipped room.
An Intelligent Wired Capsule for the Treatment of Helicobacter pylori
Authors: Luzzi Anna , Tortora Giuseppe
Published: December 2021

An endoscopic capsule is a miniaturized ingestible video camera used to acquire images of the gastrointestinal tract wirelessly. Being morphologically equivalent to any ingestible pill, they can be simply swallowed. Endoscopic capsules therefore present an inviting alternative to the traditional endoscope for the examination of the gastrointestinal tract as well for therapeutic purposes. Endoscopic capsules are considered a disruptive technology, as they have revolutionized the examination of the gastrointestinal tract in a relatively short time. The implementation of an active locomotion system can improve the performance of a capsule and, in the solution proposed in this paper, allows providing the capsule the needed power for therapeutic purposes. Alternative therapeutic solutions, based on optical solutions and capsule endoscopy can be applied to patients affected by Helicobacter pylori, a bacterium of the stomach that affects about half of the world population, mainly in developing countries. The infection can be asymptomatic or associated with slight symptomatology. In some cases, it can take to major pathologies or death. The literature reports results deriving from recent applications of photodynamic treatments to H. pylori. Specific wavelengths have been found to exhibit photo-killing capabilities toward the bacterium. Some solutions have been proposed based on the use of endoscopic devices and capsules capable of administering photodynamic therapy inside the stomach. The proposed treatments, however, are invasive and insufficient to achieve long-term eradication. In this work, the administration of photodynamic therapy is proposed, aimed at the eradication of H. pylori by means of an active endoscopic capsule with LED emission. The capsule design, in addition to the therapeutic module aimed at administering an appropriate light intensity at specific wavelengths already demonstrated in the literature, integrates an active locomotion system aimed at maximizing the efficiency of the treatment.
Laser-Induced Chemical Liquid-Phase Deposition Plasmonic Gold Nanoparticles on Porous TiO2 Film with Great Photoelectrochemical Performance
Authors: Voronin Anton S. , Nemtsev Ivan V. , Molokeev Maxim S. , Simunin Mikhail M. , Kozlova Ekaterina A. , Markovskaya Dina V. , Lebedev Denis V. , Lopatin Dmitry S. , Khartov Stanislav V.
Published: December 2021

This paper considers the photoelectrochemical characteristics of a composite porous TiO2 thin film with deposited plasmonic gold nanoparticles. The deposition of gold nanoparticles was carried out by the laser-induced chemical liquid-phase deposition (LCLD) method. The structural characteristics of the composite have been studied; it has been shown that the porous TiO2 film has a lattice related to the tetragonal system and is in the anatase phase. Gold nanoparticles form on the surface of a porous TiO2 film. A complex of photoelectrochemical measurements was carried out. It was shown that the deposition of plasmonic gold nanoparticles led to a significant increase in the photocurrent density by ~820%. The proposed concept is aimed at testing the method of forming a uniform layer of plasmonic gold nanoparticles on a porous TiO2 film, studying their photocatalytic properties for further scaling, and obtaining large area Au/TiO2/FTO photoelectrodes, including in the roll-to-roll process.
A New Radiological Risk Containment Procedure in Potentially Contaminated Areas
Authors: Caridi Francesco , Paladini Giuseppe , Venuti Valentina , Procopio Salvatore , Iannone Michelangelo , Crupi Vincenza , Majolino Domenico
Published: December 2021

A new radiological risk containment procedure, developed to manage the radiological risk in potentially contaminated areas, is presented here. This new methodological approach, systematically employed in sampling and site inspection activities in unknown areas from an environmental point of view, allowed the discovery of eight 226Ra orphan sources buried under the road surface, in a good state of conservation, in an industrial area of the Calabrian territory, southern Italy, and they are reported here as a case study. For workers performing sampling activities in areas for which information regarding the possible presence of contaminated material is missing, an in situ radiometric check is usually carried out as a potential radiological risk prevention, by measuring the levels of environmental radioactivity. Other than this, the procedure described in this article includes, as novelty, a series of progressive operations never carried out before all together for outdoor activities: the assessment of the presence (if any) of hot spots by recording radiometric anomalies, outdoor gamma spectrometry measurements in order to identify the radionuclides generating those anomalies, the sources unearthing activities, the management of the material found and the application of a risk containment protocol.
Characteristics of Volatile Organic Compounds in the Pearl River Delta Region, China: Chemical Reactivity, Source, and Emission Regions
Authors: Yang Weiqiang , Yu Qingqing , Pei Chenglei , Liao Chenghao , Liu Jianjun , Zhang Jinpu , Zhang Yanli , Qiu Xiaonuan , Zhang Tao , Zhang Yongbo , Wang Xinming
Published: December 2021

Volatile organic compounds (VOCs) are important precursors of photochemical ozone and secondary organic aerosol (SOA). Here, hourly variations of ambient VOCs were monitored with an online system at an urban site (Panyu, PY) in the Pearl River Delta region during August–September of 2020 in order to identify reactive VOC species and major sources of VOCs, OH loss rate (LOH), SOA formation potential (SOAFP), and corresponding emission source regions. The average concentration of VOCs at PY was 31.80 ± 20.82 ppbv during the campaign. The C2–C5 alkanes, aromatics, and ≥C6 alkanes contributed for the majority of VOC, alkenes and aromatics showed the highest contribution to LOH and SOAFP. Further, m/p-xylene, propene, and toluene were found to be the top three most reactive anthropogenic VOC species, with respective contributions of 11.6%, 6.1%, and 5.8% to total LOH. Toluene, m/p-xylene, and o-xylene constituted a large fraction of calculated SOAFP. Seven major sources were identified by using positive matrix factorization model. Vehicle exhaust made the most significant contribution to VOCs, followed by liquefied petroleum gas and combustion sources. However, industrial-related sources (including industrial solvent use and industrial process emission) had the largest contribution to LOH and SOAFP. By combining source contribution with wind direction and wind speed, the regions of different sources were further identified. Based on high-resolution observation data during ozone pollution, this study clearly exhibits key reactive VOC species and the major emission regions of different VOC sources, and thus benefits the accurate emission control of VOCs in the near future.
Sedimentary Cobalt Protoporphyrin as a Potential Precursor of Prosthetic Heme Group for Bacteria Inhabiting Fossil Organic Matter-Rich Shale Rock
Authors: Stasiuk Robert , Matlakowska Renata
Published: December 2021

This study hypothesizes that bacteria inhabiting shale rock affect the content of the sedimentary cobalt protoporphyrin present in it and can use it as a precursor for heme synthesis. To verify this hypothesis, we conducted qualitative and quantitative comparative analyses of cobalt protoporphyrin as well as heme, and heme iron in shale rock that were (i) inhabited by bacteria in the field, (ii) treated with bacteria in the laboratory, and with (iii) bacterial culture on synthetic cobalt protoporphyrin. Additionally, we examined the above-mentioned samples for the presence of enzymes involved in the heme biosynthesis and uptake as well as hemoproteins. We found depletion of cobalt protoporphyrin and a much higher heme concentration in the shale rock inhabited by bacteria in the field as well as the shale rock treated with bacteria in the laboratory. Similarly, we observed the accumulation of protoporphyrin in bacterial cells grown on synthetic cobalt protoporphyrin. We detected numerous hemoproteins in metaproteome of bacteria inhabited shale rock in the field and in proteomes of bacteria inhabited shale rock and synthetic cobalt protoporhyrin in the laboratory, but none of them had all the enzymes involved in the heme biosynthesis. However, proteins responsible for heme uptake, ferrochelatase and sirohydrochlorin cobaltochelatase/sirohydrochlorin cobalt-lyase were detected in all studied samples.
Sulfated Hyaluronan Modulates the Functional Properties and Matrix Effectors Expression of Breast Cancer Cells with Different Estrogen Receptor Status
Authors: Koutsakis Christos , Tavianatou Anastasia-Gerasimoula , Kokoretsis Dimitris , Baroutas Georgios , Karamanos Nikos K.
Published: December 2021

Hyaluronan (HA) is an extracellular matrix glycosaminoglycan (GAG) that plays a pivotal role in breast cancer. While HA is the only GAG not normally substituted with sulfate groups, sulfated hyaluronan (sHA) has previously been used in studies with promising antitumor results. The aim of the present study was to evaluate the effects sHA fragments have on breast cancer cells with different estrogen receptor (ER) status. To this end, ERα-positive MCF-7, and ERβ-positive MDA-MB-231 cells were treated with non-sulfated HA or sHA fragments of 50 kDa. The functional properties of the breast cancer cells and the expression of key matrix effectors were investigated. According to the results, sHA attenuates cell proliferation, migration, and invasion, while increasing adhesion on collagen type I. Furthermore, sHA modulates the expression of epithelial-to-mesenchymal transition (EMT) markers, such as e-cadherin and snail2/slug. Additionally, sHA downregulates matrix remodeling enzymes such as the matrix metalloproteinases MT1-MMP, MMP2, and MMP9. Notably, sHA exhibits a stronger effect on the breast cancer cell properties compared to the non-sulfated counterpart, dependent also on the type of cancer cell type. Consequently, a deeper understanding of the mechanism by which sHA facilitate these processes could contribute to the development of novel therapeutic strategies.
Crafting Life Stories in Photocollage: An Online Creative Art-Based Intervention for Older Adults
Authors: Keisari Shoshi , Piol Silvia , Elkarif Talia , Mola Giada , Testoni Ines
Published: December 2021

Creative arts therapies (CAT) provide a safe and creative environment for older adults to process life experiences and maintain personal growth while aging. There is a growing need to make creative arts therapies more accessible to the aging population, as many have limited access to these services. This need has been catalyzed by the advent of the COVID-19 pandemic. Tele-CAT offers a possible solution. This study explored the experiences of older adults who participated in an online creative process of digital photocollage based on CAT. Twenty-four Italian and Israeli community-dwelling older adults aged 78 to 92 participated in this research through Zoom teleconferencing software. Transcriptions of the sessions and the art produced through the photocollage were qualitatively analyzed through Thematic Analysis. The findings show that the projective stimuli of digital photographs supported older adults’ narratives and engaged them in a more embodied emotional experience. Participant experiences involved artistic enjoyment within a positive and safe interaction with therapists. The creation of digital photocollages allowed the participants to process their life experiences and create an integrative view of their life, a vital developmental task in late life. These results point to the advantages and challenges of tele-CAT for older adults.
Predicting the Performance of Concurrent Systematic Random Biopsies during Image Fusion Targeted Sampling of Multi-Parametric MRI Detected Prostate Cancer. A Prospective Study (PRESET Study)
Authors: Alqahtani Saeed , Zhang Xinyu , Wei Cheng , Zhang Yilong , Szewczyk-Bieda Magdalena , Wilson Jennifer , Huang Zhihong , Nabi Ghulam
Published: December 2021

The study was aimed to develop a predictive model to identify patients who may benefit from performing systematic random biopsies (SB) in addition to targeted biopsies (TB) in men suspected of having prostate cancer. A total of 198 patients with positive pre-biopsy MRI findings and who had undergone both TB and SB were prospectively recruited into this study. The primary outcome was detection rates of clinically significant prostate cancer (csPCa) in SB and TB approaches. The secondary outcome was net clinical benefits of SB in addition to TB. A logistic regression model and nomogram construction were used to perform a multivariate analysis. The detection rate of csPCa using SB was 51.0% (101/198) compared to a rate of 56.1% (111/198) for TB, using a patient-based biopsy approach. The detection rate of csPCa was higher using a combined biopsy (64.6%; 128/198) in comparison to TB (56.1%; 111/198) alone. This was statistically significant (p < 0.001). Age, PSA density and PIRADS score significantly predicted the detection of csPCa by SB in addition to TB. A nomogram based on the model showed good discriminative ability (C-index; 78%). The decision analysis curve confirmed a higher net clinical benefit at an acceptable threshold.
Fundamentals and Principles of Solid-State Electrochemical Sensors for High Temperature Gas Detection
Authors: Gorbova Elena , Tzorbatzoglou Fotini , Molochas Costas , Chloros Dimitris , Demin Anatoly , Tsiakaras Panagiotis
Published: December 2021

The rapid development of science, technology, and engineering in the 21st century has offered a remarkable rise in our living standards. However, at the same time, serious environmental issues have emerged, such as acid rain and the greenhouse effect, which are associated with the ever-increasing need for energy consumption, 85% of which comes from fossil fuels combustion. From this combustion process, except for energy, the main greenhouse gases-carbon dioxide and steam-are produced. Moreover, during industrial processes, many hazardous gases are emitted. For this reason, gas-detecting devices, such as electrochemical gas sensors able to analyze the composition of a target atmosphere in real time, are important for further improving our living quality. Such devices can help address environmental issues and inform us about the presence of dangerous gases. Furthermore, as non-renewable energy sources run out, there is a need for energy saving. By analyzing the composition of combustion emissions of automobiles or industries, combustion processes can be optimized. This review deals with electrochemical gas sensors based on solid oxide electrolytes, which are employed for the detection of hazardous gasses at high temperatures and aggressive environments. The fundamentals, the principle of operation, and the configuration of potentiometric, amperometric, combined (amperometric-potentiometric), and mixed-potential gas sensors are presented. Moreover, the results of previous studies on carbon oxides (COx), nitrogen oxides (NOx), hydrogen (H2), oxygen (O2), ammonia (NH3), and humidity (steam) electrochemical sensors are reported and discussed. Emphasis is given to sensors based on oxygen ion and proton-conducting electrolytes.
Dysregulation of the CD163-Haptoglobin Axis in the Airways of COPD Patients
Authors: Higham Andrew , Baker James M. , Jackson Natalie , Shah Rajesh , Lea Simon , Singh Dave
Published: December 2021

Pulmonary iron levels are increased in chronic obstructive pulmonary disease (COPD) patients. Iron causes oxidative stress and is a nutrient for pathogenic bacteria. Iron may therefore play an important role in the pathophysiology of COPD. The CD163-haptglobin axis plays a central role in the regulation of iron bioavailability. The aim of this study was to examine dysregulation of the CD163-haptglobin axis in COPD. We measured soluble CD163 (sCD163) and haptoglobin levels in sputum supernatants by enzyme-linked immunosorbent assay (ELISA) and sputum macrophage CD163 and haptoglobin expression by flow cytometry in COPD patients and controls. SCD163 levels were lower in COPD patients compared to controls (p = 0.02), with a significant correlation to forced expiratory volume in 1 s (FEV1)% predicted (rho = 0.5, p = 0.0007). Sputum macrophage CD163 expression was similar between COPD patients and controls. SCD163 levels and macrophage CD163 expression were lower in COPD current smokers compared to COPD ex-smokers. Haptoglobin levels were not altered in COPD patients but were regulated by genotype. Macrophage CD163 and haptolgobin expression were significantly correlated, supporting the role of CD163 in the cellular uptake of haptoglobin. Our data implicates a dysfunctional CD163-haptoglobin axis in COPD, which may contribute to disease pathophysiology, presumably due to reduced clearance of extracellular iron.
Adipose-Specific PPARα Knockout Mice Have Increased Lipogenesis by PASK–SREBP1 Signaling and a Polarity Shift to Inflammatory Macrophages in White Adipose Tissue
Authors: Hinds Terry D. , Kipp Zachary A. , Xu Mei , Yiannikouris Frederique B. , Morris Andrew J. , Stec Donald F. , Wahli Walter , Stec David E.
Published: December 2021

The nuclear receptor PPARα is associated with reducing adiposity, especially in the liver, where it transactivates genes for β-oxidation. Contrarily, the function of PPARα in extrahepatic tissues is less known. Therefore, we established the first adipose-specific PPARα knockout (PparaFatKO) mice to determine the signaling position of PPARα in adipose tissue expansion that occurs during the development of obesity. To assess the function of PPARα in adiposity, female and male mice were placed on a high-fat diet (HFD) or normal chow for 30 weeks. Only the male PparaFatKO animals had significantly more adiposity in the inguinal white adipose tissue (iWAT) and brown adipose tissue (BAT) with HFD, compared to control littermates. No changes in adiposity were observed in female mice compared to control littermates. In the males, the loss of PPARα signaling in adipocytes caused significantly higher cholesterol esters, activation of the transcription factor sterol regulatory element-binding protein-1 (SREBP-1), and a shift in macrophage polarity from M2 to M1 macrophages. We found that the loss of adipocyte PPARα caused significantly higher expression of the Per-Arnt-Sim kinase (PASK), a kinase that activates SREBP-1. The hyperactivity of the PASK–SREBP-1 axis significantly increased the lipogenesis proteins fatty acid synthase (FAS) and stearoyl-Coenzyme A desaturase 1 (SCD1) and raised the expression of genes for cholesterol metabolism (Scarb1, Abcg1, and Abca1). The loss of adipocyte PPARα increased Nos2 in the males, an M1 macrophage marker indicating that the population of macrophages had changed to proinflammatory. Our results demonstrate the first adipose-specific actions for PPARα in protecting against lipogenesis, inflammation, and cholesterol ester accumulation that leads to adipocyte tissue expansion in obesity.
Crimean–Congo Hemorrhagic Fever Virus Past Infections Are Associated with Two Innate Immune Response Candidate Genes in Dromedaries
Authors: Lado Sara , Futas Jan , Plasil Martin , Loney Tom , Weidinger Pia , Camp Jeremy V. , Kolodziejek Jolanta , Kannan Dafalla O. , Horin Petr , Nowotny Norbert , Burger Pamela A.
Published: December 2021

Dromedaries are an important livestock, used as beasts of burden and for meat and milk production. However, they can act as an intermediate source or vector for transmitting zoonotic viruses to humans, such as the Middle East respiratory syndrome coronavirus (MERS-CoV) or Crimean–Congo hemorrhagic fever virus (CCHFV). After several outbreaks of CCHFV in the Arabian Peninsula, recent studies have demonstrated that CCHFV is endemic in dromedaries and camel ticks in the United Arab Emirates (UAE). There is no apparent disease in dromedaries after the bite of infected ticks; in contrast, fever, myalgia, lymphadenopathy, and petechial hemorrhaging are common symptoms in humans, with a case fatality ratio of up to 40%. We used the in-solution hybridization capture of 100 annotated immune genes to genotype 121 dromedaries from the UAE tested for seropositivity to CCHFV. Through univariate linear regression analysis, we identified two candidate genes belonging to the innate immune system: FCAR and CLEC2B. These genes have important functions in the host defense against viral infections and in stimulating natural killer cells, respectively. This study opens doors for future research into immune defense mechanisms in an enzootic host against an important zoonotic disease.
Discovery and Early Clinical Development of Selective Immunoproteasome Inhibitors
Authors: Kirk Christopher J. , Muchamuel Tony , Wang Jinhai , Fan R. Andrea
Published: December 2021

Inhibitors of the proteolytic activity of the 20S proteasome have transformed the treatment of multiple B-cell malignancies. These agents have also been employed with success in the treatment of patients with autoimmune diseases and immune-mediated disorders. However, new agents are needed to fully unlock the potential of proteasome inhibitors as immunomodulatory drugs. The discovery that selective inhibitors of the immunoproteasome possess broad anti-inflammatory activity in preclinical models has led to the progression of multiple compounds to clinical trials. This review focuses on the anti-inflammatory potential of immunoproteasome inhibition and the early development of KZR-616, the first selective inhibitor of the immunoproteasome to reach clinical testing.