bluuebunny/crossref_metadata_2025 huggingface dataset:
How do the components used in chemical seed treatment affect physiological quality over the storage period?
Authors: Rocha Debora Kelli , Reis Venicius Urbano Vilela , Carvalho Everson Reis , Nardelli Amanda Carvalho Penido , Morais Giovana Murari , Reis Leandro Vilela
Published: January 2025

ABSTRACT In the soybean crop, seed treatment is widespread, often with the use of several products. The aim of this study was to evaluate the effect of phytosanitary and functional products used in such treatments on the quality of stored soybean seeds. The seeds were treated with combinations of fungicide, insecticide, polymer, finishing powder, graphite, cobalt-molybdenum (CM), and inoculant. Quality evaluations were made at 0, 15, 30, 60, and 90 days of storage after the treatment by means of the following tests: germination, seedling emergence, modified accelerated aging in substrate, primary root length, as well as quantification of dust-off of the seeds. A completely randomized experimental design was used in a 10 × 5 factorial arrangement, involving 10 seed treatment compositions and five storage periods. To avoid harmful effects on physiological quality, CM and graphite added to the phytosanitary products should be used nearer to sowing, in a period of less than 15 days. Seeds with the basic industrial seed treatment used as a technological package in most seed production companies (fungicide, insecticides, and polymer) maintained physiological quality up to 60 days of storage. An excessive number of products use as seed treatment in this study, consisting of more than five, can negatively affect seed quality, even in a short storage period of 15 days. The composition of the treatment directly affects the dust-off of the seeds, especially combinations that include finishing powder and graphite.
Phα1β interaction with the Kv11.1 potassium channel in HEK293 cells transfected with the human ERG channel
Authors: Calixto João B. , Santos Adara Aurea dos , Ferreira Juliano , Souza Alessandra Hubner , Castro Junior Célio José de , Gomez Marcus Vinicius
Published: January 2025

Abstract Background: This study examines the impact of Phα1β, a spider peptide derived from the venom of Phoneutria nigriventer, on the Kv11.1 potassium channel in HEK293 cells transfected with the human ERG potassium channel. Phα1β inhibits high-voltage calcium channels and acts as an antagonist of the TRPA1 receptor, both of which play crucial roles in pain transduction pathways. Over the past 15 years, our research has demonstrated the potential of Phα1β, in both its native and recombinant forms, as a promising analgesic drug through preclinical tests conducted on rodent pain models. Regulatory agencies require the evaluation of new drugs on human ERG channels. Methods: To assess hERG potassium channel inhibition, we utilized the FLIPR® Potassium Assay, a commercially available kit. The assay involved testing the effects of Phα1β alongside the well-established hERG potassium channel blocker dofetilide, which served as a positive control. The viability of HEK-293 cells was assessed using the colorimetric MTT reduction test (3-(4, dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), whereby viable cells reduce the MTT salt, forming a formazan complex within their mitochondria, as previously described. Results: Phα1β was tested at concentrations of 56, 225, 450, and 900 pMol, resulting in a discreet inhibition of hERG potassium channel activity at higher concentrations, approximately 13.47%, with an IC50 value exceeding 900 pMol. Dofetilide, administered at concentrations ranging from 0.0001 to 10 µM, displayed a concentration-dependent inhibition of the hERG potassium channel, with a mean IC50 value of 0.1642 µM (0.1189-0.2282 µM). To evaluate cytotoxicity, HEK293-hERG cells were exposed to Phα1β concentrations of 56/900 pMol for 24 hours, resulting in no significant alteration in cell viability. Conclusion: Our findings indicate that even at high concentrations, Phα1β does not impede the functionality of the hERG potassium channel nor affect cell viability.
Silicon sources on Cenostigma pyramidalis seed quality
Authors: Silva Lucy G. da , Bruno Riselane de L. A. , Silva Juciely G. da , Jeronimo Rayane E. de O. , Medeiros Ana K. de A. , Silva Hilderlande F. da , Nascimento Luciana C. do
Published: January 2025

ABSTRACT Seed quality is an essential characteristic for species reproduction. In this scenario, Cenostigma pyramidalis has important characteristics for the purpose of recovering degraded areas. However, because it is found in the Caatinga, this species tends to be more prone to infection by phytopathogens. Therefore, it is important to treat its seeds to prevent the incidence of fungi before and after planting. One of these alternatives is the use of silicon, which can contribute to increased vigor and disease control. In this scenario, the objective was to evaluate different sources of silicon in the control of naturally occurring fungi associated with C. pyramidalis seeds, and their physiological quality. The experiment was carried out at the Phytopathology Laboratory of the Federal University of Paraíba, Campus II, Areia, PB, Brazil. The seeds, after scarification to overcome dormancy, were treated with: T1 - Control; T2 - Captana, T3 - Agrosilício plus®; T4 - Rocksil®; T5 - Sifol®; T6 - Chelal®; and T7 - Bugram®. The experiment was conducted in a completely randomized design. The seeds were subjected to sanitary, germination, and emergence tests. 100 seeds were used per treatment for the germination and emergence tests in four replicates each with 25 seeds, while for the health test 10 replicates of 10 seeds each were used. All silicon sources were efficient in controlling Aspergillus spp., Cladosporium sp., and Penicillium sp. fungi in C. pyramidalis seeds. The treatment with Sifol® is recommended to control the incidence of fungi without affecting the physiological quality of seeds.
Surface roughness of composite resins subjected to brushing with whitening toothpastes: an in vitro study
Authors: FERREIRA Nicolle Madruga Ramos , LIPPERT Vinicius Funghetto , HECK Amanda Baptista da Silva , SPOHR Ana Maria , KUNRATH Marcel Ferreira , FELDENS Carlos Alberto , KRAMER Paulo Floriani
Published: January 2025

Abstract The emergence of toothpastes containing different abrasive and whitening substances has been a constant concern among dental professionals. The aim of the present study was to perform an in vitro assessment of the surface topography of nanoparticle composite resins subjected to simulated brushing with dentifrices. Test samples were prepared with Filtek Universal (3M ESPE), Filtek Bulkfill (3M ESPE) and Z350 (3M ESPE), with 24 samples per resin. A testing machine was used to simulate brushing with the dentifrices Colgate Total 12, Oral B 100% and Oral B Gengiva Detox Gentle Whitening (8 samples per group). The constant speed of the machine was 250 cycles per minute, and 20.000 cycles were carried out, which corresponds to 24 months (1 hour and 20 minutes). Roughness features and qualitative surface topography were investigated. Statistical analysis involved the Kruskal–Wallis, Wilcoxon and Mann–Whitney tests. A significant increase in surface roughness was found for all the resins (p < 0.05). However, no significant difference was found among the resins in terms of final roughness values (p = 0.690). In contrast, a significant difference among dentifrices was found with respect to roughness measurements (p < 0.001). The qualitative analysis revealed an increase in surface roughness in all the samples and differences in the abrasive potential of the dentifrices. In conclusion, brushing with dentifrices increases the surface roughness parameters of composite resin restorations. Moreover, the differences in the abrasive effects of the dentifrices indicate a need for further studies to establish efficacy and safety criteria.
Digital Capabilities on Business Performance: Does It Matter?
Authors: Freitas Junior José Carlos da Silva , Maçada Antonio Carlos Gastaud , Brinkhues Rafael Alfonso
Published: January 2025

ABSTRACT Objective: the study aims to address the central research question: How do digital capabilities impact the performance of digital businesses? We explore digital capabilities in the context of digital transformation, enhancing the understanding of this phenomenon for businesses through dynamic capability theory and defining digital capabilities based on previous studies. Methods: these capabilities are presented in the research model. A survey was conducted with 308 digital businesses, and the hypotheses were tested using partial least squares structural equation modeling. Results: this paper demonstrates the significant impact of digital capabilities on business performance, providing evidence that ecosystem orchestration capability is a precursor to other capabilities. Conclusions: we conclude that a critical factor for a digital business’s success is its responsiveness. Digital capabilities are essential for a company’s success in the new digital business landscape. Additionally, digital technologies enable entrepreneurs to create innovations that cross traditional industry boundaries, integrating digital and non-digital assets and scaling new ventures with new products, services, and business models. This study is valuable for managers to direct investments in digital technologies, integrate stakeholders, and respond quickly to customer demands to enhance performance.
Hemodynamic and respiratory variation in the six-minute walk test in patients undergoing cardiac surgery: a cross-sectional study
Authors: Casagrande Julia , Bodnar Emely Teixeira , Winkelmann Eliane Roseli
Published: January 2025

ABSTRACT The six-minute walk test (6MWT) is commonly prescribed for cardiac surgery patients, yet the hemodynamic and respiratory variations during the test remain insufficiently described in this population. This study aimed to compare the hemodynamic and respiratory data, dyspnea sensation variation, and perceived exertion in the lower limbs during the 6MWT between the pre- and post-test periods. It also sought to analyze whether there is a difference in the variation between the pre and postoperative periods of elective cardiac surgery for the same variables. This cross-sectional study included 113 individuals undergoing elective cardiac surgery. The 6MWT was conducted the day before surgery and on the fourth postoperative day. Results showed a significant increase in hemodynamic variables, including HR, SBP, DBP (p ≤0.01) and dyspnea and lower limb exertion (p≤0.01), both pre and postoperatively; there was no variation in SpO2 both pre- (p=0.64) and postoperatively (p=0.45). The average distance covered decreased from 346.98±107.51 to 252.06±107.17 m after surgery. There was a greater variation in the mean difference between the pre- and post- test for HR before surgery (p=0.05) and a greater feeling of perceived exertion in the lower limbs after surgery (p=0.02). Between the pre and post-test, there was a physiological increase in hemodynamic data, sensation of perceived exertion, without SpO2 alteration, and functional decline in the distance covered between the pre- and postoperative periods of cardiac surgery. HR showed greater variation preoperatively, while exertion perceived in the lower limbs did so postoperatively, although this was clinically irrelevant.
Assessment of functioning and disability of postpartum women: a study based on the International Classification of Functioning, Disability and Health
Authors: Moraes Marina da Silva , Feitoza Francisco Farias , Padilha Juliana Falcão
Published: January 2025

ABSTRACT The puerperium begins soon after placental expulsion, with local and systemic changes in the woman’s body. This transition is associated with the woman’s functioning. The International Classification of Functioning, Disability and Health (ICF) underlie the application of the biopsychosocial model. This study investigated the functioning and disability of postpartum women in the immediate, late, and remote puerperal subperiods. This is a cross-sectional, observational, quantitative study. An anamnesis form was applied with sociodemographic, anthropometric and obstetric data and with questions related to the ICF. The self-administered 36-item WHODAS 2.0 was applied to investigate disability. Qualitative variables were described by frequency and percentage distribution and quantitative variables with descriptive statistics. For group comparison, Kruskal-Wallis and Dunn’s post hoc tests were applied with 5% significance. A total of 82 postpartum women participated in the study. We obtained 32 ICF categories, with structure and function being the most frequent. In WHODAS 2.0, for total sample and for each puerperal subperiod, the (D5) domain – Activities of Life – was more compromised. When comparing domains with puerperal subperiods, immediate puerperium showed less disability, differing from remote puerperium and late puerperium for cognition 10.42 (p=0.0365) and participation 18.75 (p=0.0197). The (D2) domain – Mobility – showed a difference between late puerperium and remote puerperium, with remote puerperium having less disability 17.50 (p=0.0212). Based on the diversity of filled-in items in the ICF, it is concluded that functioning can be affected in the puerperium, and disability may be presented at all times.
Un poco de cariño. Horror y sensualidad en relatos etnográficos
Authors: Blázquez Nahuel Adrián
Published: January 2025

Resumen En este texto pretendo recuperar el lugar del horror y de la sensualidad en literatura de ficción y en diversas situaciones de mis investigaciones. La cuestión principal que busco indagar es: ¿cómo ser fiel a la voz de nuestros interlocutores cuando estos nos generan rechazo? Muchas veces, al vivenciar sus mundos debemos mirar hacia otro lado porque aquello nos produce malestar o resulta insoportable, pero al mismo tiempo captan nuestra atención. Paradojas como éstas podrían pensarse como quién «baila con el enemigo». Lejos de eso, diré que no se trata de cuestiones meramente «metodológicas», sino que implica una apuesta política y epistemológica para comprender mejor el mundo de las violencias y violaciones cometidas, porque como etnógrafos por más distancia analítica que establezcamos, de algún modo u otro nos alcanzan y repercuten. Luego, de la cuestión principal se desprende otra: ¿de qué manera lidiar con el malestar de esas voces en la etnografía? Me interesa reflexionar sobre la textualización-etnográfica y literaria-de estos mundos, porque quienes pesquisamos muertes crueles (linchamientos) o territorios turbulentos (prisiones) como los aquí retratados, solemos producir cierta fascinación en la construcción estética de nuestras etnografías. Para finalizar, observaré dos riesgos muy costosos a costa de fascinar: fetichizar la violencia e higienizar la política.
Nursing team proxemic mapping in the hemotherapy space
Authors: Gonçalves Francisco Gleidson de Azevedo , Araújo Silvia Teresa Carvalho de , Azevedo Albert Lengruber de , Ferreira Priscila Brigolini Porfírio , Torres Danelia Gómez , Costa Fernanda de Nazaré Almeida , Pires Ariane da Silva , Bastos Soraia do Socorro Furtado
Published: January 2025

ABSTRACT Objective: To analyze the influence of proxemic factors on communication and care provided by nursing professionals during transfusion in hemotherapy. Method: A descriptive, exploratory and qualitative study with 25 nursing professionals from a hospital specializing in onco-hematological diseases in Rio de Janeiro, based on a systematized script, individual records of proxemic factors described by Edward Hall and recorded situational interviews. The analysis considered data thematic content and used the SketchUp 3D Modeling Software Review program to visually demonstrate the behavioral mapping of the interaction of nursing professionals with patients during care. Results: Intimate, personal, social and public distances translated into proxemic factors such as repeated movements of shoulders, neck and head, which positively influenced nursing care in hemotherapy, favoring the identification of non-verbal and verbal manifestations of the team in interaction with patients. Conclusion: Nonverbal proxemic communication must result from awareness of the layout of physical space, immediate care actions, pertinent and appropriate proximity, continuous visual surveillance and clear recognition of expressions translated as interest, respect and zeal.
Mapeamento proxêmico da equipe de enfermagem no espaço da hemoterapia
Authors: Gonçalves Francisco Gleidson de Azevedo , Araújo Silvia Teresa Carvalho de , Azevedo Albert Lengruber de , Ferreira Priscila Brigolini Porfírio , Torres Danelia Gómez , Costa Fernanda de Nazaré Almeida , Pires Ariane da Silva , Bastos Soraia do Socorro Furtado
Published: January 2025

RESUMO Objetivo: Analisar a influência dos fatores proxêmicos na comunicação e no cuidado prestado pelos profissionais de enfermagem durante o ato transfusional na hemoterapia. Método: Estudo descritivo, exploratório e qualitativo, com 25 profissionais de enfermagem de hospital especializado em doenças onco-hematológicas do Rio de Janeiro, a partir de roteiro sistematizado, registro individual dos fatores proxêmicos descritos por Edward Hall e entrevista situacional gravada. A análise considerou o conteúdo temático dos dados e utilizou o programa SketchUp 3D Modeling Software Review para demonstrar visualmente o mapeamento comportamental da interação dos profissionais de enfermagem com os clientes durante o cuidado. Resultados: As distâncias íntimas, pessoal, social e pública traduziram fatores proxêmicos, como os movimentos repetidos de ombros, pescoço e cabeça, que influenciaram positivamente o cuidado de enfermagem na hemoterapia, favorecendo a identificação das manifestações não verbais e verbais da equipe na interação com o cliente. Conclusão: A comunicação não verbal proxêmica deve ser resultante da consciência sobre disposição do espaço físico, das ações imediatas de cuidado, da pertinente e apropriada proximidade, da vigilância contínua visual e do reconhecimento manifesto das expressões traduzidas por interesse, respeito e zelo.
São Paulo no contexto da industrialização do vestuário, durante a belle époque tropical - 1870 a 1922
Authors: Prado Luis André do
Published: January 2025

Resumo A indústria de vestuário no Brasil segue carente de pesquisas históricas, em particular sobre sua fase inicial. Contribui para este quadro a escassez de fontes que permitam esclarecer sobre seu período mais remoto, questões como: número de empreendimentos e da mão de obra empregada; maquinários, capitais, volumes produzidos etc. Este artigo se propõe a compor um curto painel sobre o período da belle époque tropical (dos anos 1870 até a 1922) quando o segmento ganhou impulso no Estado de São Paulo, que passava a alavancar a industrialização do segmento no país, ainda voltado a peças de modelagens simples e estáveis. Para tanto, tomamos por suporte pesquisa realizada por Francisco Bandeira Junior em 1909, tempo em que a mecanização (máquinas de costura, de corte etc.) já permitia reduzir custos de produção, simultaneamente ao incremento do comércio urbano, que ampliava o acesso a bens vestíveis, socialmente diferenciados pela qualidade e estética das peças; ou seja, pelo valor simbólico da moda.
O resgate das teorias do imperialismo para a análise do neoliberalismo: um debate*
Authors: Pereira Vinícius Vieira
Published: January 2025

Resumo No momento em que se observa a recorrência de trabalhos na área da Economia Política que se dedicam a analisar o capitalismo contemporâneo a partir do enfoque da construção das subjetividades, para os quais o pilar fundamental do chamado sistema neoliberal é o advento de uma nova razão de mundo, ou de um novo espírito do capitalismo, o propósito primeiro deste trabalho é o de resgatar as teorias do imperialismo, especialmente as marxistas, como ferramenta teórica de estudo da sociedade contemporânea, mostrando que elas nos permitem manter a análise do neoliberalismo no campo concreto da sociedade de classe, sem por isso perder toda a amplitude de seus desdobramentos nas demais dimensões da vida e da consciência humanas. Suscitamos o pensamento de Marx e Engels acerca dos meios da produção espiritual, n’A Ideologia Alemã, para nos auxiliar nesse debate.
Content Marketing and Digital Engagement in Amazonian Sustainable Tourism
Authors: Espinal Edwin Arango , Andrade Carlos Fernando Osorio , Pastrana Carlos Alberto Arango
Published: January 2025

ABSTRACT Objective: to analyze how different elements of content marketing on Instagram influence eWOM (measured through likes and comments) for sustainable hotels in the Amazon. Theoretical approach: the study is grounded in media richness theory and uses and gratifications theory to examine the effectiveness of social media content marketing strategies. Method: a content analysis of 1,000 Instagram posts from ten sustainable Amazon hotels was conducted, followed by a negative binomial regression model. Results: videos generate more engagement than images, validating media richness theory. Strategies focused on biodiversity and relaxation facilities are effective in generating likes, while informative and sustainability-related content encourages more comments. Cultural content showed an unexpected negative impact on engagement. Conclusion: the study contributes to the literature by providing specific recommendations on content marketing optimization in the unique context of sustainable Amazon tourism, an underexplored area in digital marketing research. The findings validate media richness and uses and gratifications theories in this specific context.
Quelante de fosfato na diálise: um estudo transversal sobre adesão dos pacientes e sobrecarga de comprimidos
Authors: Ferezin Brunelle Bruna Scavello Coelho , Araújo Luiza Karla Ramos Pereira de , Lima Carolina Marquez , Abensur Hugo , Pereira Benedito Jorge , Dalboni Maria Aparecida , Moyses Rosa Maria Affonso , Elias Rosilene Motta
Published: January 2025

Resumo Introdução: Quelantes de fosfato (P) estão entre os medicamentos mais comumente prescritos para controlar níveis de P em pacientes com DRC em diálise. Ainda há escassez de dados sobre adesão aos quelantes de P, sem comparações entre modalidades dialíticas. Métodos: Acessamos fatores associados à adesão ao quelante de P entre pacientes em diálise em um hospital universitário. A adesão foi calculada como a razão entre número de comprimidos tomados por dia, conforme relatado, e número de comprimidos prescritos. Pacientes foram considerados não aderentes se a adesão fosse ao menos 20% menor ou 30% maior do que o prescrito. Resultados: Os pacientes (N = 137) eram jovens, predominantemente mulheres, em diálise por um período mediano de 53 meses. Sevelamer e carbonato de cálcio foram prescritos como quelantes de P para 70,8% e 10,2% dos pacientes, respectivamente, sem diferença entre modalidades dialíticas (p = 0,839). P correlacionou-se com número de comprimidos prescritos (r = 0,368; p = 0,001) e número de comprimidos tomados por dia (r = 0,275; p = 0,001). Encontramos hiperfosfatemia em 52 pacientes (36,4%). A adesão ao carbonato de Ca e sevelamer foi de 100% e 68,4%, respectivamente. Pacientes não aderentes eram mulheres, mais jovens, com albumina e ureia séricas mais elevadas e menor cálcio sérico. A regressão logística mostrou que sexo feminino (HR 3,30; IC 95%: 1,39–7,84; p = 0,007) e hemodiálise vs. diálise peritoneal (HR 4,55; IC 95%: 1,26–16,39; p = 0,021) permaneceram independentemente associados ao comportamento de não adesão. Conclusão: O presente estudo sugere que estratégias para aumentar a adesão devem ser implementadas. A relação entre adesão ao quelante de fosfato e melhores desfechos merece investigação mais aprofundada.
The effects of a brief intervention at home based on shared reading to promote children’s oral language
Authors: Domeniconi Camila , Gràcia Marta , Benitez Priscila
Published: January 2025

ABSTRACT Purpose The general aim of the present study was to analyse eight mother-child interactions during shared reading with children and to assess the efficacy of a brief intervention with the mothers to promote changes in the strategies they used to develop their children’s oral language. The specific objectives were to work collaboratively with mothers, to analyse the interactions between mothers and their children before and at the end of the intervention period. Methods Mothers participated in five meetings to reflect collaboratively on strategies to promote improvements in communicative interactions in a family context and in children’s oral language and during the shared reading episodes. Standardized language tests were used to assess the oral language of children who were five and six years old and typically developing. Results The results showed that all children improved some aspects of their communicative repertoire in standardized tests and/or in the observations of natural or structured situations. Conclusion We conclude that the use of strategies such as a brief intervention to promote communicative interactions between mothers and their children may have contributed to promoting children’s oral language.
A percepção de fonoaudiólogos sobre a compreensão oral e o papel da expressividade
Authors: Ucedo Daniel De Martino , Santos Karoline Pimentel dos , Ghirardi Ana Carolina de Assis Moura , Santana Ana Paula de Oliveira
Published: January 2025

RESUMO Objetivo analisar a percepção dos fonoaudiólogos sobre o processo da compreensão oral e o papel de sua expressividade durante a avaliação na clínica fonoaudiológica. Métodos estudo transversal, analítico e qualitativo, realizado por meio de entrevista com seis fonoaudiólogos atuantes na área de linguagem, no estado de Santa Catarina. Os participantes foram recrutados por meio do método Bola de Neve, formando uma amostragem não probabilística. Após transcrição dos dados, realizou-se uma análise de conteúdo das falas dos participantes. Resultados verificou-se que a avaliação da compreensão oral foi apontada como um tema pouco discutido na formação acadêmica dos participantes e, portanto, insuficiente para prepará-los para a avaliação dessa habilidade. O conceito de compreensão oral foi relacionado, principalmente, à “audição”, ao “entendimento” e à receptividade, ao passo que a expressividade destacada por meio de menção a gestos e expressões faciais. Na prática clínica, a avaliação informal mostrou-se o método mais utilizado na avaliação da linguagem compreensiva oral, em detrimento do uso exclusivo de protocolos, e a expressividade foi evidenciada, unanimemente, entre os participantes, como critério relevante no processo avaliativo da compreensão. Conclusão a compreensão e sua relação com expressividade se apresentam como um desafio na prática clínica. Se por um lado é um tema pouco abordado nas formações acadêmicas, por outro, há poucos estudos que se propõem a discutir sobre a temática, o que se reflete na lacuna conceitual e reverbera na prática clínica. Desse modo, estudos na área da linguagem compreensiva oral são emergentes no campo da Fonoaudiologia.
Immune thrombocytopenia possibly triggered by multiple tick bites
Authors: Silva-Ramos Carlos Ramiro , Prieto-Torres Andrés Eduardo , Zuñiga Abraham Katime , Cortés-Vecino Jesús A. , Ortiz Bertha Lacouture , Cuellar Constanza , Medina-Lozano Leidy J. , Faccini-Martínez Álvaro A.
Published: January 2025

ABSTRACT Immune thrombocytopenia (ITP) is an autoimmune hematological condition characterized by a markedly isolated decrease in platelets without any apparent associated clinical conditions, resulting in bleeding and bruising of the skin, mucous membranes, and major organs. It is often triggered by preceding illness or several immune stimulants such as immunizations, infections, allergic reactions, among others. While uncommon, arthropod bites can trigger acute ITP. Four cases have been reported due to bee stings and insect bites, as well as a case of ITP following honeybee-venom therapy. Here, we report a case of acute ITP possibly triggered by multiple tick bites.
Fiber isolated from discarded fruits and vegetables reduces valproic acid-induced hepatotoxicity in rats
Authors: Ramirez Maria Rosana , Manuale Debora , Yori Juan Carlos
Published: January 2025

Abstract In this work, a methodology was developed to obtain dietary fiber from agro-industrial fruit and vegetable waste. The resulting raw material was named DF. The physicochemical analysis revealed a high total dietary fiber content, along with an appropriate soluble to insoluble fiber ratio and excellent water and fat retention capacity. Subsequently, its potential protective effect against hepatotoxicity induced by valproic acid (VPA) was investigated in Wistar rats, both as preventive and curative treatments. For this purpose, two different trials were conducted. In the preventive trial, VPA (250 mg/kg/day; oral) was administered concomitantly with DF (0.3 and 0.15 mg/kg/day) for 14 days. In the curative trial, VPA was administered for 14 days, followed by DF for an additional 14 days. The results demonstrated that DF supplementation normalized body weight, liver biomarkers and attenuated VPA-induced tissue damage, while normal liver architecture was preserved. These findings suggest that DF obtained from agro-industrial fruit and vegetable waste materials may serve as a functional feedstock to counteract the harmful effects associated with prolonged VPA treatment.
The potential of an integrated pharmaceutical care model with telepharmacy in adherence and knowledge about pharmacological treatment in patients with mental disorders: A clinical trial
Authors: Carvalho Núbia Cristina Burgo Godoi de , Modesto Ana Carolina Figueiredo , Provin Mércia Pandolfo
Published: January 2025

Abstract The level of understanding about disease and treatment is an important factor to consider in achieving favorable outcomes in patients with mental disorders, and adherence to pharmacological treatment is crucial in this regard. The aim of this study was to assess to what extent patients with MD undergoing an integrated pharmaceutical care model with telepharmacy showed improvements in factors related to treatment adherence and their level of knowledge about pharmacotherapy. A parallel randomized clinical trial was conducted with a two-arm study in two Psychosocial Care Centers located in a state capital in Brazil. Participants in the intervention group received a comprehensive pharmaceutical care model that included attitudinal, technical, and educational interventions, while the control group received standard routine care. Predictive factors for adherence to pharmacological treatment and level of medication knowledge were assessed before and after the intervention. In the intervention group (33), significant improvements were found in the scores of adherence predictors (p<0.001) and the level of knowledge of pharmacotherapy (p<0.001) from baseline to follow-up, compared to the control group (33). The telepharmacy model demonstrated effectiveness in enhancing the scores of adherence predictors for pharmacological treatment and improving the level of knowledge of pharmacotherapy among patients with mental disorders.
Molecular docking and quantitative structure-activity relationships for a series of Trypanosoma cruzi dihydroorotate dehydrogenase inhibitors
Authors: Jesus Gabriela Ciffeli de , Ribeiro Tatiana Santana , Andricopulo Adriano D. , Ferreira Leonardo Luiz Gomes
Published: January 2025

Abstract Caused by the protozoan Trypanosoma cruzi, Chagas disease affects six to seven million people worldwide, mainly in Latin America. The drugs currently available for treating the disease are ineffective during its chronic phase and have serious adverse effects. Essential for the survival of T. cruzi, the enzyme dihydroorotate dehydrogenase (DHODH) has become a key molecular target for drug discovery in Chagas disease. This study investigates the bi-dimensional and three-dimensional quantitative structure-activity relationships (QSAR) for a series of 64 T. cruzi DHODH inhibitors. The results indicate a highly predictive 2D Hologram QSAR (HQSAR) model (q 2 = 0.65, r 2 = 0.88, and r 2 pred = 0.82) that identified key molecular fragments that correlate with DHODH inhibition. Moreover, 3D Comparative Molecular Field Analysis (CoMFA) models (q 2 = 0.75, r 2 = 0.99, and r 2 pred = 0.66) pointed out the 3D molecular features that determine the activity of the inhibitors. Although restricted to a congeneric series and focused solely on 2D and 3D descriptors, these QSAR models and molecular docking analyses identified key properties and intermolecular interactions for designing and optimizing new compounds as potent T. cruzi DHODH inhibitors.
Analysis of antibacterial agents consumption in a Brazilian southeastern hospital complex from 2018 to 2021
Authors: Oliveira Giuliana Amanda de , Reis Adriano Max Moreira
Published: January 2025

Abstract Healthcare during the COVID-19 pandemic may have led to the inappropriate use of antimicrobial agents and contributed to elevated multidrug resistance. The aim of this study was to analyze the consumption of antibacterial agents at a hospital complex from 2018 to 2021. Drug utilization study using antibacterial agents consumption data. The groups of antibacterial agents most consumed in the hospital complex were carbapenems, penicillins + beta-lactamase inhibitors, beta-lactamase-resistant penicillins, glycopeptides, and fluoroquinolones. The drugs with a compound annual growth rate that increased by more than 20% from 2018 to 2021 were amikacin (50.12%), benzylpenicillin (24.33%), oral clarithromycin (51.06%), daptomycin (34.38%), polymyxin B (20.85%), and tigecycline (41.49%). According to the AWaRE classification, the antibacterial agents from the Watch group subjected to AWaRE exhibited increased consumption in all years of the study. On the other hand, there was a reduction in the access category. In turn, the consumption of antibacterial agents in the reserve category also increased. The pattern of antimicrobial consumption in the hospital complex showed increased consumption in medical clinics and intensive care units, with a predominance of carbapenems, penicillins + beta-lactamase inhibitors, beta-lactamase-resistant penicillins, glycopeptides, fluoroquinolones, and polymyxin B, reflecting the care profile and influence of the COVID-19 pandemic.
Analysis of depression in Brazil and the feasibility of incorporating pharmacogenomics as an auxiliary tool in antidepressant treatment
Authors: Cerdeira Rafaela Slaviero Pinheiro , Hirata Mario Hiroyuki
Published: January 2025

Abstract Depression has substantially increased in the last 10 years in Brazil, and mental health became a concerning issue for the public health globally, especially considering the COVID-19 pandemic. The therapeutic conduct in depression mainly relies on medication and psychotherapy. However, more than one attempt in the treatment can be considered a common procedure before finding out the right medication. This conduct leads many patients to give up following pharmacological treatment. In this scenario, pharmacogenomics emerges as a possible supporting tool of the medical treatment. This paper aims to analyze the scenario of depression in Brazil and the viability of pharmacogenomic tests implementation as a part of the medical procedure. Official data bank from the Brazilian government was used to analyze the prevalence of depressive disorder, as well as Pharmacogenomic data bases and relevant articles around this topic. Additionally, pharmacogenomic tests typically take into consideration the genes CYP2D6 and CYP2C19, which are relevant for antidepressant treatment. Data shows that these tests could help patients achieve remission of symptoms, and they could also be economically advantageous. Thus, pharmacogenomic tests present as an interesting approach for the therapeutic conduct of depression and can significantly improve the health quality of this affected population.
Plantago major leaf extract against bacteria and fungi of medical importance
Authors: Sousa Débora Silva Marques de , Ruiz Luciana da Silva , Pereira Ana Flávia Marques , Sani Alessandra Aguirra , Zapata Tatiane Baptista , Monari Gabrielle Pires de Morais , Rosa Cassiana Ferreira da , Fernandes Junior Ary , Rall Vera Lucia Mores , Prado Diego Godina , Oliveira Alberto de , Bosco Sandra de Moraes Gimenes
Published: January 2025

Abstract Antimicrobial drug resistance is a challenge to public health. Various microorganisms, including methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, carbapenemase-producing Klebsiella pneumoniae, Pythium insidiosum, and Candida auris, have developed resistance to commonly used antimicrobials in infection disease treatments. Consequently, there is an urgent need to explore and develop novel therapeutic drugs. Natural products, particularly medicinal plants, have received attention in the quest for innovative treatments for various diseases. Plantago major is a plant endowed with several biological properties, such as antibacterial, antifungal, and healing actions. This study aimed to investigate the antimicrobial activities of the methanolic extract obtained from the leaves of P. major. Microdilution assays were conducted to determine the minimum inhibitory concentration and minimum bactericidal and fungicidal concentration. Additionally, synergism with antimicrobial drugs was assessed using a time-kill curve analysis. A synergistic bactericidal interaction between the extract and imipenem was observed against carbapenemase-producing K. pneumoniae. For MRSA, a bacteriostatic synergism was noted in combinations of the extract with cephalotin and oxacillin. For C. auris, a fungistatic interaction was observed between the extract and amphotericin B. These results suggest the presence of bioactive compounds within the extract with therapeutic potential for combating infections caused by these microorganisms.
Metformin hydrochloride laden nanostructured lipid carriers: A promising strategy for skin diseases
Authors: Mahran Reem Y. , Fouad Ehab A , Tous Sozan S , Eleraky Nermin E.
Published: January 2025

Abstract Metformin hydrochloride (MTF) has pharmacological properties for managing inflammatory skin conditions. MTF is a hydrophilic medication. Accordingly, embedding MTF into lipid carriers for enhancing skin penetration presents a challenge. The study aims to optimize the loading of MTF into nanostructured lipid carriers (NLCs) using a 22 full factorial design, employing the solvent injection technique. The NLCs were evaluated for encapsulation efficiency, hydrodynamic diameter, zeta potential, and polydispersity index. Alkalinization of the aqueous phase (pH = 12.5) resulted in maximizing the entrapment of MTF within NLCs. Furthermore, the tested solid lipids impacted the encapsulation of MTF based on their hydrophilic-lipophilic balance. The optimized formulation is composed of a lipid phase incorporating beeswax (75 mg), oleic acid (25 mg), and Span 60 (1% w/w), and an aqueous phase comprised of 1% w/w Tween 80, pH 12.5. The selected formula attained an entrapment efficiency of 53.68 ± 0.27%, a particle size of 333.0 ± 6.4 nm, and a negative surface charge, indicating adequate particles` stability. DSC and Molecular docking analyses confirmed the MTF incorporation within the lipid phase. The outcomes emphasize the importance of optimizing investigations in developing a viable delivery system for MTF to boost its permeation across the skin layers.
Medicines for the management of overweight and obesity: A systematic review with network meta-analysis
Authors: Marcon Gabriela Masiero , Paiva Jhonatan Mendes , Sakiyama Mariana Delariva , Tonin Fernanda Stumpf , Fernandez-Llimos Fernando , Virtuoso Suzane , Sanches Andreia Cristina Conegero
Published: January 2025

Abstract To synthesize the evidence about pharmacologic treatment of obesity and overweight and to define the options with the best risk-benefit using the stochastic analysis of multicriteria acceptability (SMAA). The analysis addresses a systematic review (PROSPERO CRD42023423308) whose research was realized in PubMed, Scopus, and Web of Science. Randomized controlled trials were included, which verified the effects of sibutramine, orlistat, liraglutide, and semaglutide in patients with IMC ≥ 26 Kg/ m². The risk of bias analysis was performed with RoB 2.0 and the outcomes evaluated were weight loss and serious adverse events. A total of 102 studies with 45.047 participants were included. The network meta-analysis revealed that all the treatments were significantly more effective than the placebo in weight reduction. The use of semaglutide (especially 0.4 mg/day) was associated with a bigger weight loss in comparison to all the other treatments (p<0.05) and the analysis of SMAA showed a risk-benefit of 95%. Besides that, we suggest re-evaluating of sibutramine 10mg/day as a therapeutic option for patients without hypertension or cardiovascular diseases, and we demonstrate the modest weight loss promoted by orlistat 120mg, sibutramine 5mg, and liraglutide 1,8mg and advise against its use, once the benefits do not outweigh the risks.
Unraveling cerebroprotective potential of fenchone: A combined in vivo and in silico exploration of nitric oxide synthase modulation
Authors: Mamatha Bangaru , Areti Anka Rao , Prasanth DSNBK , Malothu Narender
Published: January 2025

Abstract In this study, we investigated the cerebroprotective effects of fenchone (FEN) against brain ischemia through in silico and in vivo approaches, focusing on the inhibition of nitric oxide synthase (NOS) and the modulation of oxidative stress markers. Molecular docking revealed the potential binding affinity of FEN for the NOS active site, with a binding energy of -6.6 kcal/mol. This was validated through molecular dynamics simulations over a 100 ns time frame, demonstrating stability and favorable interaction profiles. Wistar albino rats underwent bilateral common carotid artery occlusion/reperfusion (BCCAO/R) followed by FEN administration at doses of 100 and 200 mg/kg. Our results indicated a significant reduction in cerebral infarction size and improvements in electroencephalography (EEG) signal magnitude with FEN treatment. Additionally, FEN restored the activity of antioxidant enzymes (catalase and superoxide dismutase) and decreased malondialdehyde (MDA) and nitric oxide (NO) levels and infarct size compared to those in untreated ischemic rats. Histological analysis further corroborated the neuroprotective effects of FEN, demonstrating the structural preservation of neurons in the hippocampal CA1 region. Overall, the results suggest that FEN plays a neuroprotective role in brain ischemia, potentially through the inhibition of NOS, reduction of oxidative stress, and modulation of antioxidants, highlighting the potential of FEN as a therapeutic candidate for ischemic stroke management.
Screening of SIRT2 inhibitors from natural product databases using computer-aided drug design and molecular dynamics simulation
Authors: Liu Tianyi , Yin Hongli , Dong Xue , Hu Qingyang , Hu Xuejiao , Yan Wenxin , Wang Huanhuan , Li Zhong
Published: January 2025

Abstract This study is committed to searching for inhibitors of deacetylase SIRT2 within the natural product database via computer-aided drug design techniques. A comprehensive computer-aided drug design platform has been successfully established by integrating various techniques such as drug-likeness screening, pharmacokinetic prediction, molecular docking, and molecular dynamics simulation. The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (YaTCM) has been thoroughly explored to identify SIRT2 inhibitors, and the discovered compounds have been validated using molecular dynamics simulation. Through the computer-aided drug design method, five compounds capable of binding to SIRT2 have been successfully screened out from 47,696 natural product compounds derived from 6,220 herbs in the YaTCM database. Molecular dynamics simulation reveals that Artonin E and Paleatin B can form stable receptor-ligand complexes in the active pocket of SIRT2 inhibitors. Based on computer-aided drug design and virtual screening and verification techniques, Artonin E and Paleatin B have been identified as inhibitors of SIRT2, which is a key therapeutic target for the treatment of neuroblastoma. Applying computer aided drug design techniques to identify potential drug molecules from natural products holds profound significance for drug research.
Multitarget CDK inhibitors roscovitine and UCN‑01 induce apoptosis in colorectal cancer cells by inhibiting cell cycle progression and transcription
Authors: Manohar Sonal Mohan , Joshi Kalpana Sanjay
Published: January 2025

Abstract Colorectal cancer (CRC) is the third leading cause of cancer death in the world, and its incidence is steadily rising in developing nations. Cell cycle aberrations due to deregulation of cyclin dependent kinases (CDKs) and cyclins are common events during colorectal carcinogenesis. Herein, we investigate the anticancer potential of two multitarget CDK inhibitors viz. roscovitine (specific inhibitor of CDK1, 2, 7, and 9) and UCN-01 (pan CDK inhibitor) against three CRC cell lines. Both the drugs exerted cytotoxicity and inhibited clonogenic potential of human CRC cell lines. These drugs induced apoptosis, downregulated cell cycle regulatory and transcriptional CDKs and cyclins’ protein expression as well as their activity. Moreover, dual combination of either of these CDK inhibitors with standard chemotherapeutic drugs was found to be synergistic in CRC cells. Thus, we demonstrate that multiple CDK inhibition offers promising therapeutic strategy against CRC.
Assessing the impact of clinically relevant medicinal chemistry teaching: Learning gains from case study approach
Authors: Lustoza Fernanda Domingues , Fernandes João Paulo S.
Published: January 2025

Abstract Considering the unique role of medicinal chemistry in the pharmacists’ formation, in this work we assessed the impact of medicinal chemistry teaching on the interpretation of a clinical situation using statistical and learning gain analysis. Lectures were conducted with or without medicinal chemistry information about the drugs involved, and pre and post-tests were applied. Pharmacy students (n = 35) were divided into two experimental groups (control and test) and presented with a case report selected from literature involving statin-related myopathy when a pre-test was applied. A lecture containing pharmacological information on statins was delivered to the students from the control group. In contrast, the test group received the same information, but medicinal chemistry elements of statins were inserted, and afterward post-test was applied. The scores from the pre-test and post-test were statistically evaluated and used to calculate Cohen’s d, Hake’s, and Dellwo’s G learning gains to compare the performance of the students from both groups. The results showed that students from the test group obtained significantly higher performance in the post-test and higher learning gain values. In summary, medicinal chemistry elements provided important knowledge to unveil the reported clinical situation and to provide skills for adequate drug selection to avoid statin-related myopathy.