bluuebunny/crossref_metadata_2025 huggingface dataset:
Applying unsupervised machine-learning algorithms and MUSIC back-projection to characterize 2018–2022 Hualien earthquake sequence
Authors: Jian Pei-Ru , Wang Yu
Published: September 2022

The city of Hualien in eastern Taiwan is located at the junction of the Luzon arc–Eurasian continent collision and the oblique subduction of the Philippine Sea plate along the Ryukyu Trench. This complex tectonic setting creates one of the most intense seismic hazards in the world. Since the mid-twentieth century, three earthquake sequences (1951, 1986–1992, and 2018–2022) have caused severe damages and revealed a diversity of focal mechanisms, demonstrating a complex seismogenic tectonic interactions of collisions and subductions. In this study, we used the two-dimensional back-projection method to examine rupture characteristics of three strong earthquakes during the latest sequence (2018–2022). We also studied the same earthquake sequence using unsupervised machine-learning via density-based spatial clustering of applications with noise (DBSCAN) and principal component analysis (PCA). Our results revealed two westward dipping seismogenic structures. The deeper one lies beneath the eastern flank of the Central Range and the shallower one rotates clockwise along the coastline from south to north. Overall, focal mechanisms within these two structures are consistent with planar geometries obtained using PCA. The deeper structure beneath the Central Range is likely an imbricated fault system. The coastline structure is characterized by a transition of strike and dip angles from south to north. We suggest that the subhorizontal nodal planes in earthquakes offshore Hualien are related to the shallow northward dipping patch in the northern part of the coastal structure. Our Coulomb stress change results revealed strong linkages between these two structures.
Identification of pupils’ preferences of patterners and dramatists in secondary school computer science education
Authors: Bentz Anette , Standl Bernhard
Published: September 2022

With the increasing integration of computer science into school curricula, a growing number of pupils are coming into contact with this subject. To get as many pupils as possible interested in computer science, the teaching strategies and methods must meet the heterogeneous prerequisites of the pupils. The purpose of this paper is to find out how to identify two different student behavioral preferences mentioned in the computer science education literature. Considering these preferences, the focus was set on the preferences for socially enriched learning on the one hand and task-oriented learning on the other hand. Even though related research has been found on the preferences of preschoolers and adults, research is missing in the field of computer science education at the secondary school level. Thus, this study focuses on the learning preferences of pupils in secondary school education regarding the two behavioral preferences. As a result, an instrument was developed and piloted that, validated by qualitative methods, measures these different preferences. Preliminary exploratory results from analyses with school classes show that preference clusters can be found in secondary education. Further research aims to use these findings to improve computer science education.
Assessing the Feasibility and Effectiveness of a Linkage Into Mental Health Care Program for Adults Affected by Hurricane Sandy
Authors: Schwartz Rebecca M , Corley Samantha S , Rasul Rehana , Bevilacqua Kristin G , Gonzalez Adam , Gillezeau Christina , Lieberman-Cribbin Wil , Taioli Emanuela
Published: September 2022

Objective:

Research indicates that greater exposure to Hurricane Sandy is associated with increased mental health difficulties. This study examined whether Project Restoration, a program that linked adults into mental health care (L2C), was effective in reducing post-Sandy mental health difficulties as compared to a cohort of adults matched on mental health difficulties that were not linked into post-Sandy mental health care.

Methods:

Project Restoration participants (n = 52) with elevated self-reported mental health difficulties had the option to enroll into L2C. Project LIGHT (n = 63) used similar methodologies but did not have a L2C component and served as the matched control group.

Results:

Multivariable modeling showed significant decreases in all mental health difficulties except for depression in the Project Restoration group, whereas there were no significant decreases in LIGHT. The decrease in anxiety from baseline to follow-up was significantly greater for Project Restoration as compared to LIGHT.

Conclusion:

Findings confirm the powerful impact community outreach and treatment have on reducing mental health difficulties after a disaster.
Utilization of the TRAIN Learning Network for Online Disaster Medicine and Public Health Training During the COVID-19 Pandemic
Authors: Malcolm Trent R. , Plotkin Ilya , Quintanilla Norma , Schuitema Kelly , Schuler Keke , Kirsch Thomas D.
Published: September 2022

Objective:

The coronavirus disease 2019 (COVID-19) pandemic dramatically accelerated a growing trend toward online and asynchronous education and professional training, including in the disaster medicine and public health sector. This study analyzed the impact of the COVID-19 pandemic on the growth of the TRAIN Learning Network (TRAIN) for the year 2020 and evaluated pandemic-related changes in use patterns by disaster and public health professionals.

Methods:

The TRAIN database was queried to determine the change in the number of registered users, total courses completed, and courses completed related to COVID-19 during 2020.

Results:

In 2020, a total of 755,222 new users joined the platform – nearly 3 times the average added annually over the preceding 5 y (2015-2019). TRAIN users completed 3,259,074 training courses in 2020, more than double the average number of training courses that were completed annually from 2015-2019. In addition, 17.8% of all newly added disaster and public health training courses in 2020 were specifically related to COVID-19.

Conclusion:

Online education provided by TRAIN is a critical tool for just-in-time disaster health training following a disaster event or public health emergency, including in a global health crisis such as a pandemic.
What’s in a Name: How US Supreme Court Justices Shape Law and Policy in the Lower Courts
Authors: Masood Ali S. , Kassow Benjamin J.
Published: September 2022

We investigate whether inherent differences between the majority opinions of US Supreme Court justices result in certain justices being systematically more influential compared to their peers. We offer a theory in which lower court adoption of the Supreme Court’s precedents are influenced through justice opinion attributes, case characteristics, and circuit-level influences. To test the predictions, we examine the universe of responses by US Courts of Appeals to the signed majority opinions of individual justices by assembling a dataset of over 130,000 observations. We assess the interdependence of the mechanisms at work through a coarsened exact matching algorithm. We find that intricate tendencies in opinion writing disparately impact lower court attentiveness to the Supreme Court’s decisions. These findings offer new and important implications toward a richer understanding of the influence of individual justices on legal development and policy adoption in the American courts.
Strong-arm Sobriety: Addressing Precarity through Probation
Authors: Piehowski Victoria , Phelps Michelle S.
Published: September 2022

Over the past half-century, the US welfare and penal systems have become increasingly fused modes of poverty governance. At the center of the welfare-penal continuum sits probation, a form of community supervision that operates as a central hub, directing people to both services and incarceration. Drawing on interviews with 166 adults on probation in Hennepin County, Minnesota, in 2019, we argue that the coercive care of probation is structured by the broader project of controlling alcohol and drug use among the poor. Developing the concept of strong-arm sobriety, we show how the “criminal addict” trope undergirds the central processes of probation: treatment, testing, and revocation. We argue that strong-arm sobriety misreads structural precarity as the result, rather than the cause, of individuals’ choices. In doing so, strong-arm sobriety fails to address the circumstances that engender substance use and produces future subjects for coercive care.
The Rise of China's Digital Economy: An Overview
Authors: Jiang Hong , Murmann Johann Peter
Published: September 2022

To stimulate a debate about the rise of China's digital economy, this essay compares China and the US in one key area of the digital economy – e-commerce and internet-based services. China still lags behind the US in internet penetration, but it distinguishes itself by building a mobile-first, fiber-intensive, and inclusive digital infrastructure. A favorable infrastructure, innovations tailored to the large Chinese market, and local firms’ rapid commercialization of products and services turned the world's largest domestic population into active online consumers, helping China overtake the US by a large margin in retail e-commerce and digital payment. While China translated digital technologies into leading business-to-customer and customer-to-customer businesses, it has not been so successful in business-to-business services. The US is still ahead in the general-purpose technologies underlying the digital economy.
ON -ACTIONS ON K3 SURFACES IN POSITIVE CHARACTERISTIC
Authors: MATSUMOTO YUYA
Published: September 2022

In characteristic
$0$
, symplectic automorphisms of K3 surfaces (i.e., automorphisms preserving the global
$2$
-form) and non-symplectic ones behave differently. In this paper, we consider the actions of the group schemes
$\mu _{n}$
on K3 surfaces (possibly with rational double point [RDP] singularities) in characteristic p, where n may be divisible by p. We introduce the notion of symplecticness of such actions, and we show that symplectic
$\mu _{n}$
-actions have similar properties, such as possible orders, fixed loci, and quotients, to symplectic automorphisms of order n in characteristic
$0$
. We also study local
$\mu _n$
-actions on RDPs.
ADJUNCTION AND INVERSION OF ADJUNCTION
Authors: FUJINO OSAMU , HASHIZUME KENTA
Published: September 2022

We establish adjunction and inversion of adjunction for log canonical centers of arbitrary codimension in full generality.
Transportation on spheres via an entropy formula
Authors: Blower Gordon
Published: September 2022

The paper proves transportation inequalities for probability measures on spheres for the Wasserstein metrics with respect to cost functions that are powers of the geodesic distance. Let $\mu$ be a probability measure on the sphere ${\bf S}^n$ of the form $d\mu =e^{-U(x)}{\rm d}x$ where ${\rm d}x$ is the rotation invariant probability measure, and $(n-1)I+{\hbox {Hess}}\,U\geq {\kappa _U}I$, where $\kappa _U>0$. Then any probability measure $\nu$ of finite relative entropy with respect to $\mu$ satisfies ${\hbox {Ent}}(\nu \mid \mu ) \geq (\kappa _U/2)W_2(\nu,\, \mu )^2$. The proof uses an explicit formula for the relative entropy which is also valid on connected and compact $C^\infty$ smooth Riemannian manifolds without boundary. A variation of this entropy formula gives the Lichnérowicz integral.
Effect of tillage frequency, seed rate, and glyphosate application on teff and weeds in Tigray, Ethiopia
Authors: Gebrehiwot Haftamu Gebretsadik , Aune Jens Bernt , Eklo Ole Martin , Torp Torfinn , Brandsæter Lars Olav
Published: September 2022

Field experiments were conducted in 2015 and 2016 to study the effect of tillage frequency, seed rate, and glyphosate on teff and weeds. The experiments were arranged in a split plot design with three replications consisting of tillage frequency (conventional, minimum, and zero tillage) as the main plot and the combination of seed rate (5, 15, and 25 kg ha−1) and glyphosate (with and without) as subplots. Results showed that zero tillage reduced teff biomass yield by 15% compared to minimum tillage and by 26% compared to conventional tillage. Zero tillage and minimum tillage also diminished grain yield by 21% and 13%, respectively, compared to conventional tillage. Lowering the seed rate to 5 kg ha−1 reduced biomass yield by 22% and 26% compared to 15 and 25 kg ha−1, respectively. It also reduced the grain yield by around 21% compared to 15 and 25 kg ha−1 seed rates. Conventional tillage significantly diminished weed density, dry weight, and cover by 19%, 29%, and 33%, respectively, compared to zero tillage. The highest seed rate significantly reduced total weed density, dry weight, and cover by 18%, 19%, and 15%, respectively, compared to the lowest seed rate. Glyphosate did not affect weed density but reduced weed dry weight by 14% and cover by 15%. Generally, sowing teff using minimum tillage combined with glyphosate application and seed rate of 15 kg ha−1 enhanced its productivity and minimized weed effects.
Genomic prediction and genetic correlations estimated for milk production and fatty acid traits in Walloon Holstein cattle using random regression models
Authors: Paiva José Teodoro , Mota Rodrigo Reis , Lopes Paulo Sávio , Hammami Hedi , Vanderick Sylvie , Oliveira Hinayah Rojas , Veroneze Renata , Silva Fabyano Fonseca e , Gengler Nicolas
Published: September 2022

The aims of this study were to: (1) estimate genetic correlation for milk production traits (milk, fat and protein yields and fat and protein contents) and fatty acids (FA: C16:0, C18:1 cis-9, LCFA, SFA, and UFA) over days in milk, (2) investigate the performance of genomic predictions using single-step GBLUP (ssGBLUP) based on random regression models (RRM), and (3) identify the optimal scaling and weighting factors to be used in the construction of the H matrix. A total of 302 684 test-day records of 63.875 first lactation Walloon Holstein cows were used. Positive genetic correlations were found between milk yield and fat and protein yield (rg from 0.46 to 0.85) and between fat yield and milk FA (rg from 0.17 to 0.47). On the other hand, negative correlations were estimated between fat and protein contents (rg from −0.22 to −0.59), between milk yield and milk FA (rg from −0.22 to −0.62), and between protein yield and milk FA (rg from −0.11 to −0.19). The selection for high fat content increases milk FA throughout lactation (rg from 0.61 to 0.98). The test-day ssGBLUP approach showed considerably higher prediction reliability than the parent average for all milk production and FA traits, even when no scaling and weighting factors were used in the H matrix. The highest validation reliabilities (r2 from 0.09 to 0.38) and less biased predictions (b1 from 0.76 to 0.92) were obtained using the optimal parameters (i.e., ω = 0.7 and α = 0.6) for the genomic evaluation of milk production traits. For milk FA, the optimal parameters were ω = 0.6 and α = 0.6. However, biased predictions were still observed (b1 from 0.32 to 0.81). The findings suggest that using ssGBLUP based on RRM is feasible for the genomic prediction of daily milk production and FA traits in Walloon Holstein dairy cattle.
Effect of inclusion of bakery by-products in the dairy cow's diet on milk fatty acid composition
Authors: Khiaosa-ard Ratchaneewan , Kaltenegger Anna , Humer Elke , Zebeli Qendrim
Published: September 2022

Bakery by-products (BP), rich in fats and sugars, are unconventional feed sources for cows whose effects on milk fat composition have not yet been evaluated. This research paper aimed to assess the effects of dietary BP inclusion rate and feeding period on the milk fatty acid composition. Twenty-four Simmental cows were fed a diet without BP (CON) for 1 week. Then they either continued with the CON diet or switched to one of the BP diets (with 15% or 30% BP in diet dry matter) for 3 weeks. Milk samples were taken before diet change and three times during BP feeding and analysed for fatty acid composition. Data showed that increasing BP content in the diet increased total fatty acid intake, especially of 18 : 1 n9. In the milk fat, the percentages of total monounsaturated fatty acids especially of the 18 : 1 origin linearly increased with increasing dietary BP level. The percentage of fatty acids de novo synthesized in the mammary gland (the sum of 4 : 0–14 : 0) remained similar among diets (32-34% of total fatty acids). The 16 : 0 percentage dropped from 32.5 to 29.6% and from 33.6 to 28.3% for 15% and 30% BP, respectively. Only 30% BP elevated the percentage of conjugated linoleic acids (CLA: by 59%) compared with CON throughout the 3 weeks. Proportions of 18 : 2 n6 and 18 : 3 n3 and the n6:n3 ratio were unaffected by BP and feeding time. BP feeding improved all those estimated health indices of the milk fat that are suggested to be related to coronary health. In summary, the inclusion of BP in dairy rations beneficially shifted the milk fatty acid profile to more 18 : 1 fatty acids at the expense of 16 : 0. At a 30% inclusion rate, BP feeding showed an additional benefit of increased CLA content in milk fat.
Shifting policies for shifting cultivation: A history of anti-swidden interventions in Vietnam
Authors: McElwee Pamela
Published: September 2022

Swidden cultivation has long been seen as incompatible with state goals for development, modernisation and environmental protection in Vietnam. This article provides a history of anti-swidden programmes since the French colonial period: how targets were selected, how different justifications were used, how interventions were implemented, and what the impacts were. Shifts occurred over time in targets, tools, and techniques, due to leeway available to local officials and resistance of target populations, but which also prolonged overall anti-swidden campaigns by providing opportunities for continual reinvention. Shifting justifications have allowed for new funding and approaches over time, while remaining rooted in misunderstandings and cultural chauvinism.
Writing, complexity, translation
Authors: Fox Richard
Published: September 2022

Let me begin with an expression of gratitude to Verena Meyer, Thomas Hunter, Penny Edwards, Laurie Sears, Tom Patton and Kaja McGowan. It is a rare privilege to be read with such nuance and generosity by one's colleagues. I have learned much from their comments here, as I have from their work more generally. Given the brevity of my response it will have to be selective, focusing on a couple of the more prominent themes—namely, cultural complexity and translation. But I hope to return in future and think further along several of the other, very productive lines of questioning they have opened up in relation to the book.
A deliberately forgotten battle: The Lapiang Manggagawa and the Manila Port Strike of 1963
Authors: Scalice Joseph
Published: September 2022

This article documents a significant and previously unknown episode in the history of Philippine labor, the explosive Manila Port Strike of the arrastre service workers—stevedores and longshoremen—in 1963. The strike was among the largest, costliest and most politically charged labor struggles in the nation's history and yet not only has no account of it been written, it has found no mention in over a half-century of historiography. Using confidential US State Department memoranda, contemporary newspaper accounts, Partido Komunista ng Pilipinas (PKP) publications, and material published by the Lapiang Manggagawa (LM), I reconstruct the history of the strike. This article examines how an event of this magnitude, while still part of living memory, could disappear from the historical record. The case of the 1963 Port Strike highlights the need to recover the histories of the oppressed by reading not only the official archives, but also the narratives of the workers’ own organisations, against the grain.
Social Networks among Indigenous Peoples in Mexico
Authors: Skoufias Emmanuel , Lunde Trine , Patrinos Harry Anthony
Published: September 2022

We examine the extent to which social networks among indigenous peoples in Mexico have a significant effect on a variety of human capital investment and economic activities, such as school attendance and work among teenage boys and girls, and migration, welfare participation, employment status, occupation, and sector of employment among adult males and females. Using data from the 10 percent population sample of the 2000 Population and Housing Census of Mexico and the empirical strategy that Bertrand, Luttmer, and Mullainathan (2000) propose, which allows us to take into account the role of municipality and language group fixed effects, we confirm empirically that social network effects play an important role in the economic decisions of indigenous people, especially in rural areas. Our analysis also provides evidence that better access to basic services such as water and electricity increases the size and strength of network effects in rural areas.
The Color of Child Mortality in Brazil, 1950–2000: Social Progress and Persistent Racial Inequality
Authors: Wood Charles H. , Magno de Carvalho José Alberto , Horta Cláudia Júlia Guimarães
Published: September 2022

Now that racism has been officially recognized in Brazil, and some universities have adopted affirmative-action admission policies, measures of the magnitude of racial inequality and analyses that identify the factors associated with changes in racial disparities over time assume particular relevance to the conduct of public debate. This study uses census data from 1950 to 2000 to estimate the probability of death in the early years of life, a robust indicator of the standard of living among the white and Afro-Brazilian populations. Associated estimates of the average number of years of life expectancy at birth show that the 6.6-year advantage that the white population enjoyed in the 1950s remained virtually unchanged throughout the second half of the twentieth century, despite the significant improvements that accrued to both racial groups. The application of multivariate techniques to samples selected from the 1960, 1980, and 2000 census enumerations further shows that, controlling for key determinants of child survival, the white mortality advantage persisted and even increased somewhat in 2000. The article discusses evidence of continued racial inequality during an era of deep transformation in social structure, with reference to the challenges of skin color classification in a multiracial society and the evolution of debates about color, class, and discrimination in Brazil.
¿Existen brechas salariales por género en Chile?: Descomposición de las diferencias salariales entre hombres y mujeres en el contexto de regresiones por cuantiles
Authors: Perticara Marcela , Astudillo Alvaro
Published: September 2022

Este trabajo busca evaluar las brechas de salarios entre hombres y mujeres a partir de regresiones de cuantiles, utilizando los datos de la Encuesta de Protección Social 2002–2006. Las estimaciones realizadas toman en cuenta la potencial endogeneidad de la variable educación y se incluyen controles de experiencia laboral efectiva.

Se encuentra que el efecto características es pequeño y estadísticamente no significativo hasta aproximadamente el quintil 50 (mediana), donde se hace positivo (favorable a las mujeres) y crece monotónicamente hasta llegar a 12 por ciento en el percentil 90. El efecto parámetro (o componente no explicado) es siempre negativo a lo largo de toda la distribución. Notablemente, no encontramos un efecto techo en el mercado laboral chileno una vez que controlamos por la potencial endogeneidad de la variable educación. Las estimaciones intra-ocupación revelan que las mayores brechas de salarios se encuentran entre trabajadores del comercio y obreros y trabajadores agrícolas calificados.
Our Indians in Our America: Anti-Imperialist Imperialism and the Construction of Brazilian Modernity
Authors: Guzmán Tracy Devine
Published: September 2022

Indigenous peoples have been used and imagined as guardians of the Brazilian frontier since at least the mid-nineteenth century. This association was central to the foundation of the Indian Protection Service (Serviço de Proteção aos Índios, or SPI) during the early 1900s and culminated with the Amazonian Vigilance System (Sistema de Vigelância da Amazônia, or SIVAM) at the turn of the millennium. Throughout the period, the abiding desire to establish defensive dominion over disputed national territory subjected individuals and groups identified as “Indians” to the power of overlapping discourses of scientific progress, national security, and economic development. A trinity of Brazilian modernity, these goals interpellated native peoples primarily through the practice and rhetoric of education, which grounds their historical relationship with dominant national society. Drawing on SPI records, government documents, journalism, personal testimonies, and visual media, this article traces the impact of this modernist trinity on indigenist policy and in the lives of those who have been affected by its tutelary power. By transforming private indigenous spaces into public domain, Brazil's politics of anti-imperialist imperialism propagated a colonialist, metonymic relationship between “our Indians” and “our America” into the twenty-first century.
Reconstructing Indigenous Ethnicities: The Arapium and Jaraqui Peoples of the Lower Amazon, Brazil
Authors: Bolaños Omaira
Published: September 2022

In Latin America, indigenous identity claims among people not previously recognized as such by the state have become a key topic of anthropological and sociological research. Scholars have analyzed the motivations and political implications of this trend and the impacts of indigenous population's growth on national demographic indicators. However, little is known about how people claiming indigenous status constructs the meaning of their indigenous ethnicity. Drawing from sixty-four in-depth interviews, focus-group analyses, and participant observation, this article explores the double process of identity construction: the reconstruction of the Arapium indigenous identity and the creation of the Jaraqui indigenous identity in Brazil's Lower Amazon. The findings reveal six themes that contribute to the embodiment of a definition of indigenous identity and the establishment of a discursive basis to claim recognition: sense of rootedness, historical memory, historical transformation, consciousness, social exclusion, and identity politics.
‘I'm a boy, can't you see that?’: Dialogic embodiment and the construction of agency in trans youth discourse
Authors: Jones Lucy
Published: September 2022

This article offers discourse analysis of young transgender people's interaction, in which they describe being rendered powerless through misgendering or misrepresentation. It argues that the young people's collective responses to these moments enable them to challenge the ideologies underpinning their marginalisation, and to recontextualise the language used by others to describe their bodies. Stance-taking, the production of affect, and constructed dialogue are shown to be key tools in their production of an agentive, mutual identity. The article thus provides close analysis of dialogic embodiment, a process by which the body is quite literally spoken into being. By critiquing the cisnormative structures which inform and enable the young people's marginalisation, the article responds to the call for a trans linguistics (Zimman 2020) and reflects upon the author's positionality as a cisgender researcher. (Embodiment, affective stance, agency, trans identity, cisnormativity, trans linguistics)*
‘We get that’: Narrative indexicality and the construction of frustration in police stories about domestic violence victim/survivors
Authors: Andrus Jennifer , Clawson Nicole
Published: September 2022

This article considers a particular set of cultural and ideological discourses—police discourse about domestic violence (DV) victim/survivors—in a study about indexicality. Via the processes of indexicality, victim/survivors are consistently described and constructed as frustrations for police officers and police work. We pinpoint two sociosemantic structures that index frustration—the use of the word frustration and statements that initially show understanding for the victim/survivors’ situation—and then mitigate that understanding with stories about being frustrated. In the process, we argue, DV victim/survivors become indexical forms that index the social meaning that victim/survivors are frustrating rather than compliant. Further, we show how such constructions are available for reiteration by different speakers in police discourse and different contexts. The linguistic features that signal ‘frustration’ thus function in police discourse as indexical features that can be accessed and animated by police officers when they describe encounters with victim/survivors and the victim/survivors themselves. (Indexicality, narrative, police discourse, domestic violence)*
Why we need future generations: a defence of direct intergenerational reciprocity
Authors: Corvino Fausto
Published: September 2022

In this article I argue that the non-reciprocity problem does not apply to intergenerational justice. Future generations impact, here and now, on the well-being of people now living. I firstly illustrate the economic-synchronic model of direct intergenerational reciprocity (DIR): future generations allow people now living to maintain the economic system future-oriented and capital-preserving. The rational choice for people now living is to guarantee transgenerational sufficiency to future generations. I then analyse the axiological-synchronic model of DIR: future generations give meaning and value to many of the activities that people now living carry out, and this is a compelling reason for the latter to worry about the former. I argue that only the economic-synchronic model of DIR can consistently explain why we need future generations. I conclude by discussing the limits of indirect intergenerational reciprocity.
Monitoring the 2021 Mw 8.2 Alaska Earthquake by an Offshore Seismic and Fluid Pressure Observation Network and Implications for Ocean‐Crust Dynamic Coupling
Authors: Sun Tianhaozhe , Davis Earl
Published: September 2022

Ground shaking caused by earthquakes is accompanied by seafloor and sub‐seafloor formation fluid pressure variations in offshore areas, but there have been few collocated observations of these signals. In this work, we report seismic and high‐sampling‐rate fluid pressure records of the 2021 Mw 8.2 Alaska earthquake by the Ocean Networks Canada (ONC) NEPTUNE observatory at an epicentral distance of ∼2,200 km in the northeast Pacific Ocean. The system comprises observatory nodes in various tectonic environments, with each node including buried broadband seismometers, seafloor pressure sensors, and, at two nodes, borehole pressure sensors. Seismic and tsunami waveforms of the Mw 8.2 earthquake were documented in detail. Seismic seafloor pressure variations (Psf) were dominated by Rayleigh waves of periods between 5 and 50 s, with peak amplitudes of 3–4 kPa at most sites. Waveform similarity and the linear scaling between Psf and vertical ground acceleration indicate forced acceleration of the water column being dominant in governing Psf during long‐period surface‐wave arrivals, with an additional component of elastic oscillation occurring at higher frequencies (>0.1 Hz) causing extra pressure signals. Analysis of formation pressure variations due to various types of ocean loading of distinctly different frequencies (e.g., tides, tsunami, and infragravity waves) shows stable one‐dimensional vertical loading efficiencies that depend on lithology at each borehole site, with loading response being strongly influenced by the presence of free gas at shallow depths within the Cascadia accretionary prism. Inter‐site comparisons of seismic and seafloor pressure waveforms demonstrate a key role of sediment thickness in the amplification of surface wave amplitudes.
Bridging Gaps in the Climate Observation Network: A Physics‐Based Nonlinear Dynamical Interpolation of Lagrangian Ice Floe Measurements via Data‐Driven Stochastic Models
Authors: Covington Jeffrey , Chen Nan , Wilhelmus Monica M.
Published: September 2022

Modeling and understanding sea ice dynamics in marginal ice zones rely on measurements of sea ice. Lagrangian observations of ice floes provide insight into the dynamics of sea ice, the ocean, and the atmosphere. However, optical satellite images are susceptible to atmospheric noise, leading to gaps in the retrieved time series of floe positions. This paper presents an efficient and statistically accurate nonlinear dynamical interpolation framework for recovering missing floe observations. It exploits a balanced physics‐based and data‐driven construction to address the challenges posed by the high‐dimensional and nonlinear nature of the coupled atmosphere‐ice‐ocean system, where effective reduced‐order stochastic models, nonlinear data assimilation, and simultaneous parameter estimation are systematically integrated. The new method succeeds in recovering the locations, curvatures, angular displacements, and the associated strong non‐Gaussian distributions of the missing floes in the Beaufort Sea. It also accurately estimates floe thickness and recovers the unobserved underlying ocean field with an appropriate uncertainty quantification, advancing our understanding of Arctic climate.
DNA2 mutation causing multisystemic disorder with impaired mitochondrial DNA maintenance
Authors: Sun Jiayu , Su Wenwen , Deng Jianwen , Qin Yao , Wang Zhaoxia , Liu Yuhe
Published: September 2022

Purpose

To describe a novel DNA2 variant contributing to defects in mtDNA maintenance and mtDNA depletion syndrome (MDS), and the clinical and histological findings associated with this variation.

Methods

Herein, we describe the case of a patient who presented with hearing loss and myopathy, given the family history of similar findings in the father, was evaluated by sequencing of the deafness gene panel, mitochondrial genome, and the exome. Furthermore, tissue staining, mtDNA copy number detection, mtDNA sequencing, and long-range polymerase chain reaction tests were also conducted on the muscle biopsy specimen. In vitro experiments, including analyses of the mtDNA copy number; levels of ATP, ATPase, and reactive oxygen species (ROS); and the membrane potential, were performed.

Results

The DNA2 heterozygous truncating variant c. 2368C > T (p.Q790X) was identified and verified as the cause of an mtDNA copy number decrement in both functional experiments and muscle tissue analyses. These changes were accompanied by reductions in ATP, ATPase, and ROS levels.

Conclusion

The DNA2 variant was a likely cause of MDS in this patient. These findings expand the mutational spectrum of MDS and improve our understanding of the functions of DNA2 by revealing its novel role in mtDNA maintenance.
Mesenchymal stromal cells alleviate acute respiratory distress syndrome through the cholinergic anti-inflammatory pathway
Authors: Zhang Xiaoran , Wei Xuxia , Deng Yiwen , Yuan Xiaofeng , Shi Jiahao , Huang Weijun , Huang Jing , Chen Xiaoyong , Zheng Shuwei , Chen Jieying , Chen Keyu , Xu Ruiming , Wang Hongmiao , Li Weiqiang , Li Shiyue , Yi Huimin , Xiang Andy Peng
Published: September 2022

Mesenchymal stromal cells (MSCs) have been considered a promising alternative for treatment of acute respiratory distress syndrome (ARDS). However, there is significant heterogeneity in their therapeutic efficacy, largely owing to the incomplete understanding of the mechanisms underlying the therapeutic activities of MSCs. Here, we hypothesize that the cholinergic anti-inflammatory pathway (CAP), which is recognized as a neuroimmunological pathway, may be involved in the therapeutic mechanisms by which MSCs mitigate ARDS. Using lipopolysaccharide (LPS) and bacterial lung inflammation models, we found that inflammatory cell infiltration and Evans blue leakage were reduced and that the expression levels of choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT) in lung tissue were significantly increased 6 hours after MSC infusion. When the vagus nerve was blocked or α7 nicotinic acetylcholine (ACh) receptor (α7nAChR)-knockout mice were used, the therapeutic effects of MSCs were significantly reduced, suggesting that the CAP may play an important role in the effects of MSCs in ARDS treatment. Our results further showed that MSC-derived prostaglandin E2 (PGE2) likely promoted ACh synthesis and release. Additionally, based on the efficacy of nAChR and α7nAChR agonists, we found that lobeline, the nicotinic cholinergic receptor excitation stimulant, may attenuate pulmonary inflammation and alleviate respiratory symptoms of ARDS patients in a clinical study (ChiCTR2100047403). In summary, we reveal a previously unrecognized MSC-mediated mechanism of CAP activation as the means by which MSCs alleviate ARDS-like syndrome, providing insight into the clinical translation of MSCs or CAP-related strategies for the treatment of patients with ARDS.
MTG8 interacts with LHX6 to specify cortical interneuron subtype identity
Authors: Asgarian Zeinab , Oliveira Marcio Guiomar , Stryjewska Agata , Maragkos Ioannis , Rubin Anna Noren , Magno Lorenza , Pachnis Vassilis , Ghorbani Mohammadmersad , Hiebert Scott Wayne , Denaxa Myrto , Kessaris Nicoletta
Published: September 2022

Cortical interneurons originating in the embryonic medial ganglionic eminence (MGE) diverge into a range of different subtypes found in the adult mouse cerebral cortex. The mechanisms underlying this divergence and the timing when subtype identity is set up remain unclear. We identify the highly conserved transcriptional co-factor MTG8 as being pivotal in the development of a large subset of MGE cortical interneurons that co-expresses Somatostatin (SST) and Neuropeptide Y (NPY). MTG8 interacts with the pan-MGE transcription factor LHX6 and together the two factors are sufficient to promote expression of critical cortical interneuron subtype identity genes. The SST-NPY cortical interneuron fate is initiated early, well before interneurons migrate into the cortex, demonstrating an early onset specification program. Our findings suggest that transcriptional co-factors and modifiers of generic lineage specification programs may hold the key to the emergence of cortical interneuron heterogeneity from the embryonic telencephalic germinal zones.
Discovery of an orally active benzoxaborole prodrug effective in the treatment of Chagas disease in non-human primates
Authors: Padilla Angel M. , Wang Wei , Akama Tsutomu , Carter David S. , Easom Eric , Freund Yvonne , Halladay Jason S. , Liu Yang , Hamer Sarah A. , Hodo Carolyn L. , Wilkerson Gregory K. , Orr Dylan , White Brooke , George Arlene , Shen Huifeng , Jin Yiru , Wang Michael Zhuo , Tse Susanna , Jacobs Robert T. , Tarleton Rick L.
Published: September 2022

Trypanosoma cruzi, the agent of Chagas disease, probably infects tens of millions of people, primarily in Latin America, causing morbidity and mortality. The options for treatment and prevention of Chagas disease are limited and underutilized. Here we describe the discovery of a series of benzoxaborole compounds with nanomolar activity against extra- and intracellular stages of T. cruzi. Leveraging both ongoing drug discovery efforts in related kinetoplastids, and the exceptional models for rapid drug screening and optimization in T. cruzi, we have identified the prodrug AN15368 that is activated by parasite carboxypeptidases to yield a compound that targets the messenger RNA processing pathway in T. cruzi. AN15368 was found to be active in vitro and in vivo against a range of genetically distinct T. cruzi lineages and was uniformly curative in non-human primates (NHPs) with long-term naturally acquired infections. Treatment in NHPs also revealed no detectable acute toxicity or long-term health or reproductive impact. Thus, AN15368 is an extensively validated and apparently safe, clinically ready candidate with promising potential for prevention and treatment of Chagas disease.
Multiproxy study of 7500-year-old wooden sickles from the Lakeshore Village of La Marmotta, Italy
Authors: Mazzucco Niccolò , Mineo Mario , Arobba Daniele , Caramiello Rosanna , Caruso Fermé Laura , Gassin Bernard , Guilbeau Denis , Ibáñez Juan José , Morandi Lionello F. , Mozota Millán , Pichon Fiona , Portillo Marta , Rageot Maxime , Remolins Gerard , Rottoli Mauro , Gibaja Juan F.
Published: September 2022

The lakeshore site of La Marmotta is one of the most important Early Neolithic sites of Mediterranean Europe. The site is famous for the exceptional preservation of organic materials, including numerous wooden artefacts related to navigation, agriculture, textile production, and basketry. This article presents interdisciplinary research on three of the most complete and well-preserved sickles recovered from the site, yet unpublished. All the components of the tools are analysed: the stone inserts, the wooden haft and the adhesive substances used to fix the stones inside the haft. Our innovative methodology combines use-wear and microtexture analysis of stone tools through confocal microscopy, taxonomical and technological analysis of wood, gas chromatography–mass spectrometry analysis of the adhesive substances, and pollen, non-pollen palynomorphs, and phytolith analysis of the remains incorporated within the adhesive. This multiproxy approach provides a significant insight into the life of these tools, from their production to their use and abandonment, providing evidence of the species of harvested plants and the conditions of the field during the harvesting.
Metatranscriptome analysis in human papillomavirus negative cervical cancers
Authors: Ure Agustin Enrique , Lagheden Camilla , Arroyo Mühr Laila Sara
Published: September 2022

Human papillomavirus (HPV) negative cancers are associated with symptomatic detection, late-stage diagnosis, and worse prognosis. It is thus essential to investigate all possible infectious agents and biomarkers that could early identify these HPV negative cancers. We aimed to analyze and compare the metatranscriptome present in HPV positive and HPV negative cervical cancers. We analyzed the whole RNA sequencing files from 223 HPV negative cervical cancers (negativity established after confirming cervical cancer diagnosis, sample adequacy and subjecting specimens to PCR and unbiased RNA sequencing), 223 HPV positive tumors and 11 blank paraffin block pools (used as controls) using Kraken2 software. Overall, 84 bacterial genera were detected, with 6/84 genera showing a positive median number of reads/sample and being present in both cervical tumor groups (HPV positive and negative). Viral reads belonged to 63 different viral genera, with 6/63 genera showing a positive median annotated read/sample value. No significant difference among genera was detected except for the presence of alpha-papillomaviruses. Metatranscriptome of bacteria and viruses present in HPV positive and HPV negative cervical cancers show no significant difference, except for HPV. Further studies are needed to early identify this biologically distinct group of cervical cancers.
Combining donor-derived cell-free DNA and donor specific antibody testing as non-invasive biomarkers for rejection in kidney transplantation
Authors: Obrișcă Bogdan , Butiu Maria , Sibulesky Lena , Bakthavatsalam Ramasamy , Smith Kelly D. , Gimferrer Idoia , Warner Paul , Ismail Gener , Leca Nicolae
Published: September 2022

Donor specific anti-HLA antibodies (DSA) and donor-derived cell-free DNA (dd-cfDNA) have lead to substantial progress in the non-invasive monitoring of the renal allograft by being able to detect or rule out subclinical rejection and guide immunosuppressive changes. In this study we sought to analyze the clinical, de novo DSA (dnDSA) and histological determinants of dd-cfDNA levels. The study included a cohort of stable renal function kidney transplant (KT) recipients who underwent anti-HLA dnDSA and dd-cfDNA testing between September 2017-December 2019. Statistical models were constructed to detect association with predictors of dd-cfDNA levels and other clinical characteristics. 171 renal allograft recipients were tested for dd-cfDNA and dnDSA at a median 1.06 years posttransplant (IQR: 0.37–4.63). Median dd-cfDNA was 0.25% (IQR: 0.19–0.51), 18.7% of patients having a dd-cfDNA ≥ 1%. In a multivariate linear regression model the presence of dnDSA MFI ≥ 2500 was the best independent determinant of dd-cfDNA level (p < 0.001). Among patients tested, 54 had concurrent dd-cfDNA determination at the time of an allograft biopsy. dd-cfDNA had an AUC of 0.82 (95% CI 0.69–0.91; p < 0.001) and of 0.96 (95% CI 0.87–0.99) to discriminate any rejection and ABMR, respectively. After multivariate adjustment, the models that included ABMR (R = 0.82, R2 = 0.67, p < 0.001), or ptc (R = 0.79, R2 = 0.63, p < 0.001) showed the best correlation with dd-cfDNA level. We are confirming a strong association of dd-cfDNA with dnDSA and underlying alloimmune-mediated injury in renal allograft recipients in a cohort of patients with unsuspecting clinical characteristics for rejection and excellent allograft function. Our findings support the need for noninvasive biomarker surveillance in KT recipients and we propose that dd-cfDNA may complement dnDSA screening.
Stability and volatility shape the gut bacteriome and Kazachstania slooffiae dynamics in preweaning, nursery and adult pigs
Authors: Feehan Brandi , Ran Qinghong , Dorman Victoria , Rumback Kourtney , Pogranichniy Sophia , Ward Kaitlyn , Goodband Robert , Niederwerder Megan C. , Summers Katie Lynn , Lee Sonny T. M.
Published: September 2022

The gut microbiome plays important roles in the maintenance of health and pathogenesis of diseases in the growing host. In order to fully comprehend the interplay of the gut microbiome and host, a foundational understanding of longitudinal microbiome, including bacteria and fungi, development is necessary. In this study, we evaluated enteric microbiome and host dynamics throughout the lifetime of commercial swine. We collected a total of 234 fecal samples from ten pigs across 31 time points in three developmental stages (5 preweaning, 15 nursery, and 11 growth adult). We then performed 16S rRNA gene amplicon sequencing for bacterial profiles and qPCR for the fungusKazachstania slooffiae. We identified distinct bacteriome clustering according to the host developmental stage, with the preweaning stage exhibiting low bacterial diversity and high volatility amongst samples. We further identified clusters of bacteria that were considered core, increasing, decreasing or stage-associated throughout the host lifetime.Kazachstania slooffiaewas absent in the preweaning stage but peaked during the nursery stage of the host. We determined that all host growth stages contained negative correlations betweenK. slooffiaeand bacterial genera, with only the growth adult stage containing positive correlates. Our stage-associated bacteriome results suggested the neonate contained a volatile gut microbiome. Upon weaning, the microbiome became relatively established with comparatively fewer perturbations in microbiome composition. Differential analysis indicated bacteria might play distinct stage-associated roles in metabolism and pathogenesis. The lack of positive correlates and sharedK. slooffiae-bacteria interactions between stages warranted future research into the interactions amongst these kingdoms for host health. This research is foundational for understanding how bacteria and fungi develop singularly, as well as within a complex ecosystem in the host’s gut environment.