bluuebunny/crossref_metadata_2025 huggingface dataset:
Monthly and Diurnal Variation of the Concentrations of Aerosol Surface Area in Fukuoka, Japan, Measured by Diffusion Charging Method
Authors: Kiriya Miho , Okuda Tomoaki , Yamazaki Hana , Hatoya Kazuki , Kaneyasu Naoki , Uno Itsushi , Nishita Chiharu , Hara Keiichiro , Hayashi Masahiko , Funato Koji , Inoue Kozo , Yamamoto Shigekazu , Yoshino Ayako , Takami Akinori
Published: June 2017

Observation of the ambient aerosol surface area concentrations is important to understand the aerosol toxicity because an increased surface area may be able to act as an enhanced reaction interface for certain reactions between aerosol particles and biological cells, as well as an extended surface for adsorbing and carrying co-pollutants that are originally in gas phase. In this study, the concentration of aerosol surface area was measured from April 2015 to March 2016 in Fukuoka, Japan. We investigated the monthly and diurnal variations in the correlations between the aerosol surface area and black carbon (BC) and sulfate concentrations. Throughout the year, aerosol surface area concentration was strongly correlated with the concentrations of BC, which has a relatively large surface area since BC particles are usually submicron agglomerates consisting of much smaller (tens of nanometers) sized primary soot particles. The slopes of the regression between the aerosol surface area and BC concentrations was highest in August and September 2015. We presented evidence that this was caused by an increase in the proportion of airmasses that originated on the main islands of Japan. This may enhance the introduction of the BC to Fukuoka from the main islands of Japan which we hypothesize to be relatively fresh or “uncoated”, thereby maintaining its larger surface area.
Hypermethylation of Synphilin-1, Alpha-Synuclein-Interacting Protein (SNCAIP) Gene in the Cerebral Cortex of Patients with Sporadic Parkinson’s Disease
Authors: Dashtipour Khashayar , Tafreshi Ali , Adler Charles , Beach Thomas , Chen Xin , Serrano Geidy , Tashiro Stephanie , Wang Charles
Published: June 2017

Objective: To determine and compare DNA methylation patterns between patients with Parkinson’s disease (PD) and age- and sex-similar matched non-PD controls. Background: Epigenetic regulation is one of the major mechanisms for an organism to respond to the environment through changes in gene expression and has been implicated in numerous disease processes. We would like to examine epigenetic modification patterns that may predispose or protect against PD. Methods: Frozen tissue samples of the human cerebral cortex from 12 PD patients and 12 subjects without PD pathology were obtained. Genome-wide DNA methylation profiling was performed using the Illumina HumanMethylation450 BeadChip array. Differential methylation was defined as a mean methylation level difference (delta β) of at least 0.20 (Δβ ≥ 0.20). Methylation regions with an absolute delta β value ≥ 0.20 were selected for further gene function studies. Results: We identified 2795 differentially methylated CpG sites in the frontal cortex of PD cases with a detection p-value of ≤ 0.01 and 328 differentially methylated CpG sites with a detection p-value of ≤ 0.001. A pattern of robust hypermethylation of synphilin-1, α-synuclein-interacting protein (SNCAIP) gene was found in the brain of PD cases (p = 4.93 × 10−7 and delta β = 0.60). Conclusion: Our findings support a link between SNCAIP methylation and PD risk. Hypomethylation of SNCAIP may function to protect against PD. The current results may suggest that the methylation status of SNCAIP could be useful as a marker in PD diagnosis and treatment and warrants further investigation.
Sino-Nasal 5 Questionnaire is Associated with Poor Asthma Control in Children with Asthma
Authors: Kilaikode Sasikumar , Shukla Prateek , Phull Gurpreet , Jackson James , Prue Dominique , Martinez Claudia , Scheffey Krista , Pillai Dinesh
Published: June 2017

Up to 80% of asthmatic children may experience upper airway symptoms which are often perceived as coming from the lower airways. Currently, there are no validated questionnaires to assess upper airway contribution to pediatric asthma symptoms. The Sino-Nasal 5 (SN-5) questionnaire was previously validated for identifying radiographic confirmed sinus disease in children. In this study, we hypothesize that significant SN-5 scores (≥3.5) are associated with abnormal National Asthma Education and Prevention Program (NAEPP) based asthma impairment and control in asthmatic children. Retrospective data collected on children with asthma referred for pulmonary evaluation included age, gender, ethnicity, NAEPP asthma severity, asthma control (Test for Respiratory and Asthma Control in Kids (TRACK) < 5 years, Asthma Control Test (ACT) 5 years) and pulmonary function testing. Associations between SN-5 scores and asthma impairment and control were identified. Seventy-six children were evaluated; 38% were female with a mean age of 6.9 years. Significant SN-5 scores were associated with decreased control of daytime symptoms (odds ratio (OR): 0.16 (95% confidence interval (CI): 0.06–0.44)), night time awakenings (0.09 (0.03–0.29)), activity interference (0.2 (0.06–0.68)), NAEPP defined asthma control (0.32 (0.12–0.85)) and poor asthma control based on TRACK (p < 0.001) and ACT (p < 0.001). This suggests upper airways may play a larger role in perceived lower airway symptoms, and SN-5 may be beneficial in assessing the contribution of upper airway conditions on asthma control.
A High Resolution Dataset of Drought Indices for Spain
Authors: Vicente-Serrano Sergio M. , Tomas-Burguera Miquel , Beguería Santiago , Reig Fergus , Latorre Borja , Peña-Gallardo Marina , Luna M. Yolanda , Morata Ana , González-Hidalgo José C.
Published: June 2017

Drought indices are essential metrics for quantifying drought severity and identifying possible changes in the frequency and duration of drought hazards. In this study, we developed a new high spatial resolution dataset of drought indices covering all of Spain. The dataset includes seven drought indices, spans the period 1961–2014, and has a spatial resolution of 1.1 km and a weekly temporal resolution. A web portal has been created to enable download and visualization of the data. The data can be downloaded as single gridded points for each drought index, but the entire drought index dataset can also be downloaded in netCDF4 format. The dataset will be updated for complete years as the raw meteorological data become available.
Conjugate Representations and Characterizing Escort Expectations in Information Geometry
Authors: Wada Tatsuaki , Matsuzoe Hiroshi
Published: June 2017

Based on the maximum entropy (MaxEnt) principle for a generalized entropy functional and the conjugate representations introduced by Zhang, we have reformulated the method of information geometry. For a set of conjugate representations, the associated escort expectation is naturally introduced and characterized by the generalized score function which has zero-escort expectation. Furthermore, we show that the escort expectation induces a conformal divergence.
Influence of High Pressure and Temperature on the Mechanical Behavior and Permeability of a Fractured Coal
Authors: Akbarzadeh Kasani Hossein , Chalaturnyk Richard
Published: June 2017

Understanding mechanical behavior and permeability of coal at ambient and high temperature is key in optimizing high-temperature in-situ processes such as underground coal gasification. The main objectives of this study were to characterize thermal deformation, stress-strain behavior, and gas permeability of coal samples acquired from the Genesee coal mine in Central Alberta, Canada under various temperatures and confining stresses. These measurements were conducted in a high-pressure high-temperature triaxial apparatus. Initial thermal expansion of the coal was followed by contraction in both axial and lateral directions at about 140 °C. This temperature corresponds to occurrence of pyrolysis in the coal. All specimens showed brittle behavior during shear while forming complex shear planes. The specimens exhibited compressional volumetric strain responses at all temperatures. Deformation localization initiated at various stage during shearing. Specimens sheared at 200 °C showed higher peak stresses and larger axial strains compared to those tested at room temperature (24 °C). Fluctuations of permeability were observed with confining stress and temperature. Permeability dropped at 80 °C due to thermal expansion of coal and closure of initial fractures; however, it increased at 140 and 200 °C due to a combined response of thermal expansion and pyrolysis. Small axial strain during shear was observed to reduce permeability.
Next Generation Real-Time Smart Meters for ICT Based Assessment of Grid Data Inconsistencies
Authors: Sanduleac Mihai , Lipari Gianluca , Monti Antonello , Voulkidis Artemis , Zanetto Gianluca , Corsi Antonello , Toma Lucian , Fiorentino Giampaolo , Federenciuc Dumitru
Published: June 2017

The latest technological developments are challenging for finding new solutions to mitigate the massive integration of renewable-based electricity generation in the electrical networks and to support new and dynamic energy and ancillary services markets. Smart meters have become ubiquitous equipment in the low voltage grid, enabled by the decision made in many countries to support massive deployments. The smart meter is the only equipment mandatory to be mounted when supplying a grid connected user, as it primarily has the function to measure delivered and/or produced energy on its common coupling point with the network, as technical and legal support for billing. Active distribution networks need new functionalities, to cope with the bidirectional energy flow behaviour of the grid, and many smart grid requirements need to be implemented in the near future. However there is no real coupling between smart metering systems and smart grids, as there is not yet a synergy using the opportunity of the high deployment level in smart metering. The paper presents a new approach for managing the smart metering and smart grid orchestration by presenting a new general design based on an unbundled smart meter (USM) concept, labelled as next generation open real-time smart meters (NORM), for integrating the smart meter, phasor measurement unit (PMU) and cyber-security through an enhanced smart metering gateway (SMG). NORM is intended to be deployed everywhere at the prosumer’s interface to the grid, as it is usually now done with the standard meter. Furthermore, rich data acquired from NORM is used to demonstrate the potential of assessing grid data inconsistencies at a higher level, as function to be deployed in distribution security monitoring centers, to address the higher level cyber-security threats, such as false data injections and to allow secure grid operations and complex market activities at the same time. The measures are considering only non-sensitive data from a privacy perspective, and is therefore able to be applied everywhere in the grid, down to the end-customer level, where a citizen’s personal data protection is an important aspect.
Data–Driven Fault Diagnosis of a Wind Farm Benchmark Model
Authors: Simani Silvio , Castaldi Paolo , Farsoni Saverio
Published: June 2017

The fault diagnosis of wind farms has been proven to be a challenging task, and motivates the research activities carried out through this work. Therefore, this paper deals with the fault diagnosis of a wind park benchmark model, and it considers viable solutions to the problem of earlier fault detection and isolation. The design of the fault indicator involves data-driven approaches, as they can represent effective tools for coping with poor analytical knowledge of the system dynamics, noise, uncertainty, and disturbances. In particular, the proposed data-driven solutions rely on fuzzy models and neural networks that are used to describe the strongly nonlinear relationships between measurement and faults. The chosen architectures rely on nonlinear autoregressive with exogenous input models, as they can represent the dynamic evolution of the system over time. The developed fault diagnosis schemes are tested by means of a high-fidelity benchmark model that simulates the normal and the faulty behaviour of a wind farm installation. The achieved performances are also compared with those of a model-based approach relying on nonlinear differential geometry tools. Finally, a Monte-Carlo analysis validates the robustness and reliability of the proposed solutions against typical parameter uncertainties and disturbances.
A Stochastic Programming Approach for the Planning and Operation of a Power to Gas Energy Hub with Multiple Energy Recovery Pathways
Authors: Mukherjee Ushnik , Maroufmashat Azadeh , Narayan Apurva , Elkamel Ali , Fowler Michael
Published: June 2017

There is a need for energy storage to improve the efficiency and effectiveness of energy distribution with the increasing penetration of renewable energy sources. Among the various energy storage technologies being developed, ‘power-to-gas’ is one such concept which has gained interest due to its ability to provide long term energy storage and recover the energy stored through different energy recovery pathways. Incorporation of such systems within the energy infrastructure requires analysis of the key factors influencing the operation of electrolyzers and hydrogen storage. This study focusses on assessing the benefits power-to-gas energy storage while accounting for uncertainty in the following three key parameters that could influence the operation of the energy system: (1) hourly electricity price; (2) the number of fuel cell vehicles serviced; and (3) the amount of hydrogen refueled. An hourly time index is adopted to analyze how the energy hub should operate under uncertainty. The results show that there is a potential economic benefit for the power-to-gas system if it is modeled using the two-stage stochastic programming approach in comparison to a deterministic optimization study. The power-to-gas system also offers environmental benefits both from the perspective of the producer and end user of hydrogen.
A High-Power DC-DC Converter Topology for Battery Charging Applications
Authors: Lee Il-Oun , Lee Jun-Young
Published: June 2017

A DC-DC converter that can be applied for battery chargers with the power-capacity of over 7-kW for electric vehicles (EVs) is presented in this paper. Due to a new architecture, the proposed converter achieves a reduction of conduction losses at the primary side by as much as 50% and has many benefits such as much smaller circulating current, less duty-cycle loss, and lower secondary-voltage stress. In addition, its power handing capacity can be upsized easily with the use of two full-bridge inverters and two transformers. Besides, all the switches in the converter achieve zero-voltage switching (ZVS) during whole battery charging process, and the size of output filter can be significantly reduced. The circuit configuration, operation, and relevant analysis are presented, followed by the experiment on a prototype realized with a 7-kW charger. The experimental results validate the theoretical analysis and show the effectiveness of the proposed converter as battery charger.
Fifty-Six Years of Forest Development Following the 1938 Hurricane in Hillsborough, New Hampshire, USA
Authors: Allen Bruce , Seaboyer Ingeborg
Published: June 2017

Forest development patterns following the 1938 hurricane were evaluated in 45 continuous forest inventory (CFI) plots monitored from 1955 to 2011 at the Caroline A. Fox Research and Demonstration Forest (Fox), in Hillsborough, New Hampshire. Long-term plot data provide a record of landscape-level changes in a managed forest setting. Changes in density, basal area, mortality, removal, and recruitment demonstrate the effects of forest management on growth and forest structure through time. Tree density peaked in 2001 at 716.1 trees/hectare, but the basal area continued to increase from 18.8 m2/ha in 1955 to 44.7 m2/ha 2011 despite forest management activities. Hemlock and red maple dominate stem recruitment. Tree mortality rates have increased from 0.26%/year 1955–1965 to 1.03%/year 2001–2011, while removal rates have dropped from 1.04%/year to 0.44%/year.
Evaluation of Variations in Frequency of Landslide Events Affecting Pyroclastic Covers in Campania Region under the Effect of Climate Changes
Authors: Rianna Guido , Reder Alfredo , Mercogliano Paola , Pagano Luca
Published: June 2017

In recent years, pyroclastic covers mantling slopes in the Campania region of southern Italy have frequently been affected by flowslides. Due to high exposure and demographic pressure in these areas, assessment of the potential effects of climate change on the frequency of such events has become a crucial issue. In this regard, our paper proposes a simulation chain comprising three main elements: (i) climate simulation at the highest horizontal resolution available for Italy (8 km); (ii) a bias correction procedure in an attempt to remove systematic errors in the entire weather forcing probability distribution; (iii) the data obtained used as input for an interpretative tool estimating the evolution of soil pore water pressure and water storage (bulk water content) by means of a well-calibrated coupled thermo-hydraulic approach able to adequately take into account soil-atmosphere interaction dynamics. The predictive ability of the geotechnical model to reproduce failure conditions was tested by forcing it with temperature and precipitation observations. Subsequently, the performance of the entire modeling chain was evaluated for a period from 1981 to 2010. Lastly, variations in landslide occurrence were assessed up to 2100 under two concentration scenarios. An increase with different features was estimated under both scenarios depending on the time horizon and the severity of the concentration scenario.
FGF-2 Gene Polymorphism in Osteoporosis among Guangxi’s Zhuang Chinese
Authors: Bin Xiaoyun , Lin Chaowen , Huang Xiufeng , Zhou Qinghui , Wang Liping , Xian Cory
Published: June 2017

Osteoporosis is a complex multifactorial disorder of gradual bone loss and increased fracture risk. While previous studies have shown the importance of many genetic factors in determining peak bone mass and fragility fractures and in suggesting involvement of fibroblast growth factor-2 (FGF-2) in bone metabolism and bone mass, the relationship of FGF-2 genetic diversity with bone mass/osteoporosis has not yet been revealed. The current study investigated the potential relevance of FGF-2 gene polymorphism in osteoporosis among a Zhuang ethnic Chinese cohort of 623, including 237 normal bone mass controls, 227 osteopenia, and 159 osteoporosis of different ages. Bone density was examined by calcaneus ultrasound attenuation measurement, and single nucleotide polymorphisms (SNPs) and linkage disequilibrium analyses were performed on five SNP loci of FGF-2 gene. Significant differences were found in bone mass in males between the 45-year-old and ≥70-year-old groups (p < 0.01), and in females among 55, 60, 65 and 70-year-old groups (p < 0.05). Males had higher bone mass values than females in the same age (over 55-year-old) (p < 0.05). The proportions of individuals with normal bone mass decreased with age (65.2% to 40% in males, and 50% to 0% in females), whereas prevalence of osteoporosis increased with age (15.4% to 30% in men, and 7.7% to 82% in women). Out of five FGF-2 SNP loci, the TA genotype of rs308442 in the osteoporosis group (40.2%) was higher than in the control group (29.5%) (p < 0.05). The TA genotype was significantly correlated with the risk of osteoporosis (odds ratio OR = 1.653), 95% confidence interval (CI): 1.968–1.441). Strong linkage disequilibrium in FGF-2 gene was also detected between rs12644427 and rs3747676, between rs12644427 and rs3789138, and between rs3747676 and rs3789138 (D’ > 0.8, and r2 > 0.33). Thus, the rs308442 locus of FGF-2 gene is closely correlated to osteoporosis in this Zhuang ethnic Chinese cohort, and the TA may be the risk genotype of osteoporosis.
Multiple Isoforms of ANRIL in Melanoma Cells: Structural Complexity Suggests Variations in Processing
Authors: Sarkar Debina , Oghabian Ali , Bodiyabadu Pasani , Joseph Wayne , Leung Euphemia , Finlay Graeme , Baguley Bruce , Askarian-Amiri Marjan
Published: June 2017

The long non-coding RNA ANRIL, antisense to the CDKN2B locus, is transcribed from a gene that encompasses multiple disease-associated polymorphisms. Despite the identification of multiple isoforms of ANRIL, expression of certain transcripts has been found to be tissue-specific and the characterisation of ANRIL transcripts remains incomplete. Several functions have been associated with ANRIL. In our judgement, studies on ANRIL functionality are premature pending a more complete appreciation of the profusion of isoforms. We found differential expression of ANRIL exons, which indicates that multiple isoforms exist in melanoma cells. In addition to linear isoforms, we identified circular forms of ANRIL (circANRIL). Further characterisation of circANRIL in two patient-derived metastatic melanoma cell lines (NZM7 and NZM37) revealed the existence of a rich assortment of circular isoforms. Moreover, in the two melanoma cell lines investigated, the complements of circANRIL isoforms were almost completely different. Novel exons were also discovered. We also found the family of linear ANRIL was enriched in the nucleus, whilst the circular isoforms were enriched in the cytoplasm and they differed markedly in stability. With respect to the variable processing of circANRIL species, bioinformatic analysis indicated that intronic Arthrobacter luteus (Alu) restriction endonuclease inverted repeats and exon skipping were not involved in selection of back-spliced exon junctions. Based on our findings, we hypothesise that “ANRIL” has wholly distinct dual sets of functions in melanoma. This reveals the dynamic nature of the locus and constitutes a basis for investigating the functions of ANRIL in melanoma.
Benefits of Argan Oil on Human Health—May 4–6 2017, Errachidia, Morocco
Authors: Lizard Gérard , Filali-Zegzouti Younes , Midaoui Adil El
Published: June 2017

The First International Symposium “The beneficial effects of argan oil on human health” is part of the dynamics of research and development programs on argan oil, which prompts Morocco to deploy various strategies, programs and plans for the protection and preservation of the argan tree and of its know-how that is linked to the production of argan oil.[...]
Issues of Acute Kidney Injury Staging and Management in Sepsis and Critical Illness: A Narrative Review
Authors: Nusshag Christian , Weigand Markus , Zeier Martin , Morath Christian , Brenner Thorsten
Published: June 2017

Acute kidney injury (AKI) has a high incidence on intensive care units around the world and is a major complication in critically ill patients suffering from sepsis or septic shock. The short- and long-term complications are thereby devastating and impair the quality of life. Especially in terms of AKI staging, the determination of kidney function and the timing of dialytic AKI management outside of life-threatening indications are ongoing matters of debate. Despite several studies, a major problem remains in distinguishing between beneficial and unnecessary “early” or even harmful renal replacement therapy (RRT). The latter might prolong disease course and renal recovery. AKI scores, however, provide an insufficient outcome-predicting ability and the related estimation of kidney function via serum creatinine or blood urea nitrogen (BUN)/urea is not reliable in AKI and critical illness. Kidney independent alterations of creatinine- and BUN/urea-levels further complicate the situation. This review critically assesses the current AKI staging, issues and pitfalls of the determination of kidney function and RRT timing, as well as the potential harm reflected by unnecessary RRT. A better understanding is mandatory to improve future study designs and avoid unnecessary RRT for higher patient safety and lower health care costs.
Reuse of Pillaring Agent in Sequential Bentonite Pillaring Processes
Authors: Bertella Francine , Pergher Sibele
Published: June 2017

This work describes the synthesis and characterization of pillared clays using a new pillaring method: the reuse of the pillaring solution. First, an Al pillared clay (PILC) was synthesized, and after filtration, the pillaring agent was stored and reused for an additional three pillaring procedures (P1, P2, and P3). The filtered pillaring solution was stored for one year and then reused for one additional pillaring procedure (P4). The samples were analyzed using XRD, N2 physisorption measurements and chemical analysis (EDX). All of the samples exhibited basal spacings larger than 17 Å and BET surface areas greater than 160 m2/g. After the P4 pillaring, the pillaring agent was precipitated with a Na2SO4 solution, and the resulting solid was analyzed using XRD and SEM. The results indicated that even after a total of five pillaring procedures, Al13 ions were still present in solution. Therefore, it is possible to reuse the pillaring solution four times and to even store the solution for one year, which is important from an industrial perspective.
Effect of Core-shell Ceria/Poly(Vinylpyrrolidone) (PVP) Nanoparticles Incorporated in Polymer Films and Their Optical Properties (2): Increasing the Refractive Index
Authors: Itoh Toshio , Uchida Toshio , Izu Noriya , Shin Woosuck
Published: June 2017

We investigated the preparation of well-dispersed core-shell ceria-poly(vinylpyrrolidone) (PVP) nanoparticles with an average particle size of around 20 nm which were used to produce a hybrid film with a polymer coating of dipentaerythritol hexaacrylate (DPHA). We obtained good dispersion of the nanoparticles in a mixed solvent of 48% 1-methoxy-2-propanol (MP), 32% 3-methoxy-3-methyl-1-butanol (MMB), and 20% methyl i-butyl ketone (MIBK). An ink of the polymer coating consisting of 68.7 wt% nanoparticles and 31.3 wt% DPHA with a polymerization initiator was prepared using this solvent mixture. The surface of the hybrid film showed low roughness and the nanoparticles formed a densely packed structure in the DPHA matrix. The resulting coating possessed excellent transparency and a high refractive index of 1.69.
Studies on Mathematical Models of Wet Adhesion and Lifetime Prediction of Organic Coating/Steel by Grey System Theory
Authors: Meng Fandi , Liu Ying , Liu Li , Li Ying , Wang Fuhui
Published: June 2017

A rapid degradation of wet adhesion is the key factor controlling coating lifetime, for the organic coatings under marine hydrostatic pressure. The mathematical models of wet adhesion have been studied by Grey System Theory (GST). Grey models (GM) (1, 1) of epoxy varnish (EV) coating/steel and epoxy glass flake (EGF) coating/steel have been established, and a lifetime prediction formula has been proposed on the basis of these models. The precision assessments indicate that the established models are accurate, and the prediction formula is capable of making precise lifetime forecasting of the coatings.
A Study on Thermal and Nanomechanical Performance of Cellulose Nanomaterials (CNs)
Authors: Yildirim Nadir , Shaler Stephen
Published: June 2017

Wood-based cellulose nanomaterials (CNs) (specifically, cellulose nanofibrils (CNFs) and cellulose nanocrystals (CNCs)) are environmentally sourced low-impact materials with remarkable thermal, mechanical, and physical properties. This uniqueness makes them great candidates for creating nanocomposite materials with a wide range of attributes. Investigating the morphological, thermal, and nanomechanical properties of CNs becomes crucial to intelligent development of novel composite materials. An atomic force microscope equipped with a nanoindenter was used to investigate the compression modulus of CNFs and CNCs using two analytical approaches (denoted as Oliver Pharr (OP) and Fused Silica (FS)). The CNC modulus values (ECNC-FS = 21.1 GPa, ECNC-OP = 28.7 GPa) were statistically larger than those obtained from CNFs (ECNF-FS = 12.4 GPa, ECNF-OP = 15.1 GPa). Additionally, the FS analytical approach provided statistically significant lower estimates. Thermal stability of CNFs and CNCs was investigated using thermogravimetric analysis. Significant differences were found between CNF and CNC onset temperatures (OnsetCNC = 228.2 °C, OnsetCNF = 279.9 °C), decomposition temperatures (DTGACNC = 247.9 °C, DTGACNF = 331.4 °C), and residues (ResidueCNC = 34.4%, ResidueCNF = 22.8%). This research enriches the information on thermal stability and nanomechanical performance of cellulose nanomaterials, and provides increased knowledge on understanding the effect of CNs as a matrix or reinforce in composites.
Effect of Nanosized NbC Precipitates on Hydrogen Diffusion in X80 Pipeline Steel
Authors: Cui Qiaoqi , Wu Junsheng , Xie Donghan , Wu Xiaoguang , Huang Yunhua , Li Xiaogang
Published: June 2017

In this paper, the effects of dispersed 3~10 nm NbC precipitates on hydrogen diffusion in X80 pipeline steel were investigated by means of high resolution transmission electron microscopy (HRTEM), electrochemical hydrogen permeation, and thermal desorption spectroscopy (TDS). The relationship between hydrogen diffusion and temperature was determined for Nb-free X80 and 0.055 wt% Nb X80 steel. The temperature dividing reversible and irreversible traps was measured, and the quantity of hydrogen captured by different traps was calculated. Three types of hydrogen trap were designed and applied in the test, and the results revealed that irreversible hydrogen traps formed by nanosized and coherent NbC precipitates markedly hindered hydrogen diffusion, and prolonged breakthrough time in Nb-bearing X80 steel.
A Validation Approach for Quasistatic Numerical/Experimental Indentation Analysis in Soft Materials Using 3D Digital Image Correlation
Authors: Felipe-Sesé Luis , López-Alba Elías , Hannemann Benedikt , Schmeer Sebastian , Diaz Francisco A.
Published: June 2017

A quasistatic indentation numerical analysis in a round section specimen made of soft material has been performed and validated with a full field experimental technique, i.e., Digital Image Correlation 3D. The contact experiment specifically consisted of loading a 25 mm diameter rubber cylinder of up to a 5 mm indentation and then unloading. Experimental strains fields measured at the surface of the specimen during the experiment were compared with those obtained by performing two numerical analyses employing two different hyperplastic material models. The comparison was performed using an Image Decomposition new methodology that makes a direct comparison of full-field data independently of their scale or orientation possible. Numerical results show a good level of agreement with those measured during the experiments. However, since image decomposition allows for the differences to be quantified, it was observed that one of the adopted material models reproduces lower differences compared to experimental results.
Magnetism of Molecular Conductors
Authors: Almeida Manuel
Published: June 2017

The study of the magnetic properties of molecular conductors has experienced, during the last decades, a very significant evolution, comprising systems of increasing molecular complexity and moving towards multifunctional materials, namely by their incorporation in conducting networks of different paramagnetic centers.[...]
The Deep-Sea Polyextremophile Halobacteroides lacunaris TB21 Rough-Type LPS: Structure and Inhibitory Activity towards Toxic LPS
Authors: Lorenzo Flaviana Di , Palmigiano Angelo , Paciello Ida , Pallach Mateusz , Garozzo Domenico , Bernardini Maria-Lina , Cono Violetta La , Yakimov Michail M. , Molinaro Antonio , Silipo Alba
Published: June 2017

The structural characterization of the lipopolysaccharide (LPS) from extremophiles has important implications in several biomedical and therapeutic applications. The polyextremophile Gram-negative bacterium Halobacteroides lacunaris TB21, isolated from one of the most extreme habitats on our planet, the deep-sea hypersaline anoxic basin Thetis, represents a fascinating microorganism to investigate in terms of its LPS component. Here we report the elucidation of the full structure of the R-type LPS isolated from H. lacunaris TB21 that was attained through a multi-technique approach comprising chemical analyses, NMR spectroscopy, and Matrix-Assisted Laser Desorption Ionization (MALDI) mass spectrometry. Furthermore, cellular immunology studies were executed on the pure R-LPS revealing a very interesting effect on human innate immunity as an inhibitor of the toxic Escherichia coli LPS.
Flexural Properties and Fracture Behavior of Nanoporous Alumina film by Three-Point Bending Test
Authors: Chen Jung-Hsuan , Luo Wen-Shiang
Published: June 2017

This study investigated the influence of porosity on the flexural property of a nanoporous alumina film. When the porosity of the alumina film increased, both bending strength and modulus declined. The results from the bending test revealed that the setting of the film during the bending test had significant influence on the flexural property. Fracture only occurred when the porous side of the alumina film suffered tensile stress. The ability to resist fracture in the barrier layer was higher than in the porous side; the magnitude of the bending strength was amplified when the barrier layer sustained tensile stress. When the porous layer suffered a tensile stress, the bending strength decreased from 182.4 MPa to 47.7 Mpa as the porosity increased from 22.7% to 51.7%; meanwhile, the modulus reduced from 82.7 GPa to 17.9 GPa. In this study, the most important finding from fractographic analysis suggested that there were a localized plastic deformations and layered ruptures at the porous side of the alumina film when a load was applied. The fracture behavior of the nanoporous alumina film observed in the present work was notably different from general ceramic materials and might be related to its asymmetric nanostructure.
In Vitro Anti-Leishmanial Activity of Essential Oils Extracted from Vietnamese Plants
Authors: Le Thanh Binh , Beaufay Claire , Nghiem Duc Trong , Mingeot-Leclercq Marie-Paule , Quetin-Leclercq Joëlle
Published: June 2017

Leishmania mexicana is one of the pathogens causing cutaneous leishmaniasis which is associated with patient morbidity. In our researches for new safe and effective treatments, thirty-seven essential oils (EOs) extracted from Vietnamese plants were screened in vitro for the first time on Leishmania mexicana mexicana (Lmm) promastigotes at the maximum concentration of 50 nL/mL. Active EOs were also analyzed for cytotoxicity on mammalian cell lines (WI38, J774) and their selectivity indices (SI) were calculated. Their composition was determined by GC-MS and GC-FID. Our results indicated that EOs extracted from Cinnamomum cassia, Zingiber zerumbet, Elsholtzia ciliata and Amomum aromaticum, possessed a moderate anti-leishmanial activity, with IC50 values of 2.92 ± 0.08, 3.34 ± 0.34, 8.49 ± 0.32 and 9.25 ± 0.64 nL/mL respectively. However, they also showed cytotoxicity with SI < 10. The most promising EO was extracted from Ocimum gratissimum, displaying an IC50 of 4.85 ± 1.65 nL/mL and SI > 10. It contained 86.5% eugenol, which was demonstrated to be effective on Lmm with IC50 of 2.57 ± 0.57 nL/mL and not toxic on mammalian cells, explaining the observed activity.
Nortriterpenoids from the Fruiting Bodies of the Mushroom Ganoderma resinaceum
Authors: Chen Xian-Qiang , Chen Ling-Xiao , Zhao Jing , Tang Yu-Ping , Li Shao-Ping
Published: June 2017

Ganoderma resinaceum is usually used as ethnomedicine for immune-regulation, hyperglycemia, and liver disease. To date, only a few chemical constituents have been reported from G. resinaceum. In this study, fifteen nortriterpenoids including six new nortriterpenoids (1–6) and nine known analogs (7–15), were separated and purified from the fruiting bodies of G. resinaceum. New compounds were identified as lucidone I (1), lucidone J (2), lucidone K (3), lucidone I (4), ganosineniol B (5), and ganosineniol C (6), based on analysis of extensive spectroscopic data (high resolution mass spectrometry (HRMS), nuclear magnetic resonance (NMR), infrared (IR), and ultraviolet (UV)). The known compounds were assigned as lucidone A (7), lucidone B (8), lucidone H (9), lucidone E (10), lucidone F (11), lucidone D (12), lucidone C (13), ganoderense F (14), and ganosineniol A (15), by comparing their spectroscopic data with those reported in the literature. Compounds 3, 4, and 7–13 were examined for α-glucosidase inhibitory activity and display no significant activity, but the finding may support that the side chain of ganoderma triterpenoids played an important role in α-glucosidase inhibitory activity.
Cell-Free Production of Pentacyclic Triterpenoid Compound Betulinic Acid from Betulin by the Engineered Saccharomyces cerevisiae
Authors: Wu Jianan , Niu Yongwu , Bakur Abdelmoneim , Li Hao , Chen Qihe
Published: June 2017

Betulinic acid is a product of plant secondary metabolism which has shown various bioactivities. Several CYP716A subfamily genes were recently characterized encoding multifunctional oxidases capable of C-28 oxidation. CYP716A12 was identified as betulin C-28 oxidase, capable of modifying betulin. This study aimed to induce the transformation of betulin to betulinic acid by co-expressing enzymes CYP716A12 from Medicago truncatula and ATR1 from Arabidopsis thaliana in Saccharomyces cerevisiae. The microsome protein extracted from the transgenic yeast successfully catalyzed the transformation of betulin to betulinic acid. We also characterized the optimization of cell fragmentation, protein extraction method, and the conversion conditions. Response surface methodology was implemented, and the optimal yield of betulinic acid reached 18.70%. After optimization, the yield and the conversion rate of betulin were increased by 83.97% and 136.39%, respectively. These results may present insights and strategies for the sustainable production of betulinic acid in multifarious transgenic microbes.
Effects on Rotational Dynamics of Azo and Hydrazodicarboxamide-Based Rotaxanes
Authors: Saura-Sanmartin Adrian , Martinez-Espin Juan , Martinez-Cuezva Alberto , Alajarin Mateo , Berna Jose
Published: June 2017

The synthesis of novel hydrogen-bonded [2]rotaxanes having two pyridine rings in the macrocycle and azo- and hydrazodicarboxamide-based templates decorated with four cyclohexyl groups is described. The different affinity of the binding sites for the benzylic amide macrocycle and the formation of programmed non-covalent interactions between the interlocked components have an important effect on the dynamic behavior of these compounds. Having this in mind, the chemical interconversion between the azo and hydrazo forms of the [2]rotaxane was investigated to provide a chemically-driven interlocked system enable to switch its circumrotation rate as a function of the oxidation level of the binding site. Different structural modifications were carried out to further functionalize the nitrogen of the pyridine rings, including oxidation, alkylation or protonation reactions, affording interlocked azo-derivatives whose rotation dynamics were also analyzed.
Fabrication and Cytotoxicity of Gemcitabine-Functionalized Magnetite Nanoparticles
Authors: Popescu Roxana Cristina , Andronescu Ecaterina , Vasile Bogdan Ștefan , Truşcă Roxana , Boldeiu Adina , Mogoantă Laurențiu , Mogoșanu George Dan , Temelie Mihaela , Radu Mihai , Grumezescu Alexandru Mihai , Savu Diana
Published: June 2017

Nanotechnology has been successfully used for the fabrication of targeted anti-cancer drug carriers. This study aimed to obtain Fe3O4 nanoparticles functionalized with Gemcitabine to improve the cytotoxic effects of the chemotherapeutic substance on cancer cells. The (un) functionalized magnetite nanoparticles were synthesized using a modified co-precipitation method. The nanoconjugate characterization was performed by XRD, SEM, SAED and HRTEM; the functionalizing of magnetite with anti-tumor substances has been highlighted through TGA. The interaction with biologic media has been studied by means of stability and agglomeration tendency (using DLS and Zeta Potential); also, the release kinetics of the drug in culture media was evaluated. Cytotoxicity of free-Gemcitabine and the obtained nanoconjugate were evaluated on human BT 474 breast ductal carcinoma, HepG2 hepatocellular carcinoma and MG 63 osteosarcoma cells by MTS. In parallel, cellular morphology of these cells were examined through fluorescence microscopy and SEM. The localization of the nanoparticles related to the cells was studied using SEM, EDX and TEM. Hemolysis assay showed no damage of erythrocytes. Additionally, an in vivo biodistribution study was made for tracking where Fe3O4@Gemcitabine traveled in the body of mice. Our results showed that the transport of the drug improves the cytotoxic effects in comparison with the one produced by free Gemcitabine for the BT474 and HepG2 cells. The in vivo biodistribution test proved nanoparticle accumulation in the vital organs, with the exception of spleen, where black-brown deposits have been found. These results indicate that our Gemcitabine-functionalized nanoparticles are a promising targeted system for applications in cancer therapy.
Does Vitamin C Influence Neurodegenerative Diseases and Psychiatric Disorders?
Authors: Kocot Joanna , Luchowska-Kocot Dorota , Kiełczykowska Małgorzata , Musik Irena , Kurzepa Jacek
Published: June 2017

Vitamin C (Vit C) is considered to be a vital antioxidant molecule in the brain. Intracellular Vit C helps maintain integrity and function of several processes in the central nervous system (CNS), including neuronal maturation and differentiation, myelin formation, synthesis of catecholamine, modulation of neurotransmission and antioxidant protection. The importance of Vit C for CNS function has been proven by the fact that targeted deletion of the sodium-vitamin C co-transporter in mice results in widespread cerebral hemorrhage and death on post-natal day one. Since neurological diseases are characterized by increased free radical generation and the highest concentrations of Vit C in the body are found in the brain and neuroendocrine tissues, it is suggested that Vit C may change the course of neurological diseases and display potential therapeutic roles. The aim of this review is to update the current state of knowledge of the role of vitamin C on neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, multiple sclerosis and amyotrophic sclerosis, as well as psychiatric disorders including depression, anxiety and schizophrenia. The particular attention is attributed to understanding of the mechanisms underlying possible therapeutic properties of ascorbic acid in the presented disorders.
The Diuretic Action of Weak and Strong Alcoholic Beverages in Elderly Men: A Randomized Diet-Controlled Crossover Trial
Authors: Polhuis Kristel , Wijnen Annemarthe , Sierksma Aafje , Calame Wim , Tieland Michael
Published: June 2017

With ageing, there is a greater risk of dehydration. This study investigated the diuretic effect of alcoholic beverages varying in alcohol concentration in elderly men. Three alcoholic beverages (beer (AB), wine (AW), and spirits (S)) and their non-alcoholic counterparts (non-alcoholic beer (NAB), non-alcoholic wine (NAW), and water (W)) were tested in a diet-controlled randomized crossover trial. For the alcoholic beverages, alcohol intake equaled a moderate amount of 30 g. An equal volume of beverage was given for the non-alcoholic counterpart. After consumption, the urine output was collected every hour for 4 h and the total 24 h urine output was measured. AW and S resulted in a higher cumulative urine output compared to NAW and W during the first 4 h (effect size: 0.25 mL p < 0.003, effect size: 0.18 mL, p < 0.001, respectively), but not after the 24h urine collection (p > 0.40, p > 0.10). AB and NAB did not differ at any time point (effect size: −0.02 mL p > 0.70). For urine osmolality, and the sodium and potassium concentration, the findings were in line. In conclusion, only moderate amounts of stronger alcoholic beverages, such as wine and spirits, resulted in a short and small diuretic effect in elderly men.
Vitamin C, Aging and Alzheimer’s Disease
Authors: Monacelli Fiammetta , Acquarone Erica , Giannotti Chiara , Borghi Roberta , Nencioni Alessio
Published: June 2017

Accumulating evidence in mice models of accelerated senescence indicates a rescuing role of ascorbic acid in premature aging. Supplementation of ascorbic acid appeared to halt cell growth, oxidative stress, telomere attrition, disorganization of chromatin, and excessive secretion of inflammatory factors, and extend lifespan. Interestingly, ascorbic acid (AA) was also found to positively modulate inflamm-aging and immunosenescence, two hallmarks of biological aging. Moreover, ascorbic acid has been shown to epigenetically regulate genome integrity and stability, indicating a key role of targeted nutrition in healthy aging. Growing in vivo evidence supports the role of ascorbic acid in ameliorating factors linked to Alzheimer’s disease (AD) pathogenesis, although evidence in humans yielded equivocal results. The neuroprotective role of ascorbic acid not only relies on the general free radical trapping, but also on the suppression of pro-inflammatory genes, mitigating neuroinflammation, on the chelation of iron, copper, and zinc, and on the suppression of amyloid-beta peptide (Aβ) fibrillogenesis. Epidemiological evidence linking diet, one of the most important modifiable lifestyle factors, and risk of Alzheimer's disease is rapidly increasing. Thus, dietary interventions, as a way to epigenetically modulate the human genome, may play a role in the prevention of AD. The present review is aimed at providing an up to date overview of the main biological mechanisms that are associated with ascorbic acid supplementation/bioavailability in the process of aging and Alzheimer’s disease. In addition, we will address new fields of research and future directions.
Analyzing the Gaver—Lewis Pareto Process under an Extremal Perspective
Authors: Ferreira Marta , Ferreira Helena
Published: June 2017

Pareto processes are suitable to model stationary heavy-tailed data. Here, we consider the auto-regressive Gaver–Lewis Pareto Process and address a study of the tail behavior. We characterize its local and long-range dependence. We will see that consecutive observations are asymptotically tail independent, a feature that is often misevaluated by the most common extremal models and with strong relevance to the tail inference. This also reveals clustering at “penultimate” levels. Linear correlation may not exist in a heavy-tailed context and an alternative diagnostic tool will be presented. The derived properties relate to the auto-regressive parameter of the process and will provide estimators. A comparison of the proposals is conducted through simulation and an application to a real dataset illustrates the procedure.