bluuebunny/crossref_metadata_2025 huggingface dataset:
How Convolutional Neural Network Architecture Biases Learned Opponency and Color Tuning
Authors: Harris Ethan , Mihai Daniela , Hare Jonathon
Published: January 2021

Recent work suggests that changing convolutional neural network (CNN) architecture by introducing a bottleneck in the second layer can yield changes in learned function. To understand this relationship fully requires a way of quantitatively comparing trained networks. The fields of electrophysiology and psychophysics have developed a wealth of methods for characterizing visual systems that permit such comparisons. Inspired by these methods, we propose an approach to obtaining spatial and color tuning curves for convolutional neurons that can be used to classify cells in terms of their spatial and color opponency. We perform these classifications for a range of CNNs with different depths and bottleneck widths. Our key finding is that networks with a bottleneck show a strong functional organization: almost all cells in the bottleneck layer become both spatially and color opponent, and cells in the layer following the bottleneck become nonopponent. The color tuning data can further be used to form a rich understanding of how color a network encodes color. As a concrete demonstration, we show that shallower networks without a bottleneck learn a complex nonlinear color system, whereas deeper networks with tight bottlenecks learn a simple channel opponent code in the bottleneck layer. We develop a method of obtaining a hue sensitivity curve for a trained CNN that enables high-level insights that complement the low-level findings from the color tuning data. We go on to train a series of networks under different conditions to ascertain the robustness of the discussed results. Ultimately our methods and findings coalesce with prior art, strengthening our ability to interpret trained CNNs and furthering our understanding of the connection between architecture and learned representation. Trained models and code for all experiments are available at https://github.com/ecs-vlc/opponency.
Rethinking John 1:1
Authors: Atkins Christopher S.
Published: January 2021

The presence of wisdom “with” God at or before creation is well attested in Jewish sapiential traditions. Given the widespread recognition that the logos of John’s prologue corresponds with sophia in such traditions, it has become natural to read John 1:1b as virtually all English translations do—that is, as “and the word was with God.” Through comparative analysis of the role of divine intermediary figures in Middle-Platonism and Philo of Alexandria, this article argues against the majority interpretation by providing new arguments and a new conceptual framework for the reading, “and the Word was Godward.”
The Global South and Norm Advocacy at the United Nations Human Rights Council
Authors: Voss Michael Joel
Published: January 2021

Historically, critics of the United Nations Human Rights Council commonly focus on the perceived negative role that states from the Global South play in Council proceedings. These ‘Western-centric’ assessments fail to consider the Global South’s role in advancing their own human rights preferences. This article describes the Global South’s norm advocacy at the Council from 2006 through 2017 using thematic resolutions, an understudied but preferred tool of the Global South at the Council. This article divides thematic resolutions into three possible motivations for Global South advocacy – in response to domestic issues, international issues, and cultural differences between regions, and includes an exemplary case study from each category. The article shows the Global South is very active in human rights promotion but chooses to focus on human rights that differ from the Global North.
Decadal Modulation of the ENSO–Indian Ocean Basin Warming Relationship during the Decaying Summer by the Interdecadal Pacific Oscillation
Authors: Liu Fangyu , Zhang Wenjun , Jin Fei-Fei , Hu Suqiong
Published: January 2021

Many previous studies have shown that an Indian Ocean basin warming (IOBW) occurs usually during El Niño–Southern Oscillation (ENSO) decaying spring to summer seasons through modifying the equatorial zonal circulation. Decadal modulation associated with the interdecadal Pacific oscillation (IPO) is further investigated here to understand the nonstationary ENSO–IOBW relationship during ENSO decaying summer (July–September). During the positive IPO phase, significant warm sea surface temperature (SST) anomalies are observed over the tropical Indian Ocean in El Niño decaying summers and vice versa for La Niña events, while these patterns are not well detected in the negative IPO phase. Different decaying speeds of ENSO associated with the IPO phase, largely controlled by both zonal advective and thermocline feedbacks, are suggested to be mainly responsible for these different ENSO–IOBW relationships. In contrast to ENSO events in the negative IPO phase, the ones in the positive IPO phase display a slower decaying speed and delay their transitions both from a warm to a cold state and a cold to a warm state. The slower decay of El Niño and La Niña thereby helps to sustain the teleconnection forcing over the equatorial Indian Ocean and corresponding SST anomalies there can persist into summer. This IPO modulation of the ENSO–IOBW relationship carries important implications for the seasonal prediction of the Indian Ocean SST anomalies and associated summer climate anomalies.
The landscape of lncRNAs in Cydia pomonella provides insights into their signatures and potential roles in transcriptional regulation
Authors: Xing Longsheng , Xi Yu , Qiao Xi , Huang Cong , Wu Qiang , Yang Nianwan , Guo Jianyang , Liu Wanxue , Fan Wei , Wan Fanghao , Qian Wanqiang
Published: January 2021

Background

Long noncoding RNAs (lncRNAs) have emerged as an important class of transcriptional regulators in cellular processes. The past decades have witnessed great progress in lncRNA studies in a variety of organisms. The codling moth (Cydia pomonella L.) is an important invasive insect in China. However, the functional impact of lncRNAs in this insect remains unclear. In this study, an atlas of codling moth lncRNAs was constructed based on publicly available RNA-seq datasets.

Results

In total, 9875 lncRNA transcripts encoded by 9161 loci were identified in the codling moth. As expected, the lncRNAs exhibited shorter transcript lengths, lower GC contents, and lower expression levels than protein-coding genes (PCGs). Additionally, the lncRNAs were more likely to show tissue-specific expression patterns than PCGs. Interestingly, a substantial fraction of the lncRNAs showed a testis-biased expression pattern. Additionally, conservation analysis indicated that lncRNA sequences were weakly conserved across insect species, though additional lncRNAs with homologous relationships could be identified based on synteny, suggesting that synteny could be a more reliable approach for the cross-species comparison of lncRNAs. Furthermore, the correlation analysis of lncRNAs with neighbouring PCGs indicated a stronger correlation between them, suggesting potential cis-acting roles of these lncRNAs in the regulation of gene expression.

Conclusions

Taken together, our work provides a valuable resource for the comparative and functional study of lncRNAs, which will facilitate the understanding of their mechanistic roles in transcriptional regulation.
Virtual controls as an alternative to randomized controlled trials for assessing efficacy of interventions
Authors: Strayhorn Joseph M.
Published: January 2021

Randomized controlled trials are ubiquitously spoken of as the “gold standard” for testing interventions and establishing causal relations. This article presents evidence for two premises. First: there are often major problems with randomized designs; it is by no means true that the only good design is a randomized design. Second: the method of virtual controls in some circumstances can and should replace randomized designs.

Randomized trials can present problems with external validity or generalizability; they can be unethical; they typically involve much time, effort, and expense; their assignments to treatment conditions often can be maintained only for limited time periods; examination of their track record reveals problems with reproducibility on the one hand, and lack of overwhelming superiority to observational methods on the other hand.

The method of virtual controls involves ongoing efforts to refine statistical models for prediction of outcomes from measurable variables, under conditions of no treatment or current standard of care. Research participants then join a single-arm study of a new intervention. Each participant’s data, together with the formulas previously generated, predict that participant’s outcome without the new intervention. These outcomes are the “virtual controls.” The actual outcomes with intervention are compared with the virtual control outcomes to estimate effect sizes. Part of the research product is the prediction equations themselves, so that in clinical practice, individual treatment decisions may be aided by quantitative answers to the questions, “What is estimated to happen to this particular patient with and without this treatment?”

The method of virtual controls is especially indicated when rapid results are of high priority, when withholding intervention is likely harmful, when adequate data exist for prediction of untreated or standard of care outcomes, when we want to let people choose the treatment they prefer, when tailoring treatment decisions to individuals is desirable, and when real-world clinical information can be harnessed for analysis.
2019 novel coronavirus disease with secondary ischemic stroke: two case reports
Authors: Fu Bin , Chen Yun , Li Ping
Published: January 2021

Background

The COVID-19 pandemic, which broke out in Wuhan in 2019, has become the global health crisis of our time. Elderly patients with certain fundamental diseases are more likely to develop severe cases. The secondary lesion following viral infection have only rarely been reported.

Case presentation

We here report two cases of coronavirus-infected pneumonia with acute ischemic stroke in middle-aged patients. In both COVID-19 cases, neurological physical examinations showed normal results before infection. Lymphocytopenia, accompanied by elevated cytokines and D-dimers, were found from serum clinical laboratory examination at admission. Dysarthria and limb muscle weakness are initial manifestations, occurring one week after infect-causative pathogen, SARS-CoV-2. The head CT and head/neck arterial CTA showed small-vessel occlusion. The patients were diagnosed with coronavirus diseases with secondary acute ischemic stroke. They were treated with tirofiban and followed up with daily aspirin and atorvastatin.

Conclusions

These cases suggested that secondary ischemic stroke, mainly manifested as small-vessel occlusion, should be considered for COVID-19 patients and diagnosed and treated promptly.
EGFR inhibitors switch keratinocytes from a proliferative to a differentiative phenotype affecting epidermal development and barrier function
Authors: Joly-Tonetti Nicolas , Ondet Thomas , Monshouwer Mario , Stamatas Georgios N.
Published: January 2021

Background

Cutaneous adverse drug reactions (CADR) associated with oncology therapy involve 45–100% of patients receiving kinase inhibitors. Such adverse reactions may include skin inflammation, infection, pruritus and dryness, symptoms that can significantly affect the patient’s quality of life. To prevent severe skin damages dose adjustment or drug discontinuation is often required, interfering with the prescribed oncology treatment protocol. This is particularly the case of Epidermal Growth Factor Receptor inhibitors (EGFRi) targeting carcinomas. Since the EGFR pathway is pivotal for epidermal keratinocytes, it is reasonable to hypothesize that EGFRi also affect these cells and therefore interfere with the epidermal structure formation and skin barrier function.

Methods

To test this hypothesis, the effects of EGFRi and Vascular Endothelial Growth Factor Receptor inhibitors (VEGFRi) at therapeutically relevant concentrations (3, 10, 30, 100 nM) were assessed on proliferation and differentiation markers of human keratinocytes in a novel 3D micro-epidermis tissue culture model.

Results

EGFRi directly affect basal keratinocyte growth, leading to tissue size reduction and switching keratinocytes from a proliferative to a differentiative phenotype, as evidenced by decreased Ki67 staining and increased filaggrin, desmoglein-1 and involucrin expression compared to control. These effects lead to skin barrier impairment, which can be observed in a reconstructed human epidermis model showing a decrease in trans-epidermal water loss rates. On the other hand, pan-kinase inhibitors mainly targeting VEGFR barely affect keratinocyte differentiation and rather promote a proliferative phenotype.

Conclusions

This study contributes to the mechanistic understanding of the clinically observed CADR during therapy with EGFRi. These in vitro results suggest a specific mode of action of EGFRi by directly affecting keratinocyte growth and barrier function.
Assessment of various continual reassessment method models for dose-escalation phase 1 oncology clinical trials: using real clinical data and simulation studies
Authors: James G. D. , Symeonides S. , Marshall J. , Young J. , Clack G.
Published: January 2021

Background

The continual reassessment method (CRM) identifies the maximum tolerated dose (MTD) more efficiently and identifies the true MTD more frequently compared to standard methods such as the 3 + 3 method. An initial estimate of the dose-toxicity relationship (prior skeleton) is required, and there is limited guidance on how to select this. Previously, we compared the CRM with six different skeletons to the 3 + 3 method by conducting post-hoc analysis on a phase 1 oncology study (AZD3514), each CRM model reduced the number of patients allocated to suboptimal and toxic doses. This manuscript extends this work by assessing the ability of the 3 + 3 method and the CRM with different skeletons in determining the true MTD of various “true” dose-toxicity relationships.

Methods

One thousand studies were simulated for each “true” dose toxicity relationship considered, four were based on clinical trial data (AZD3514, AZD1208, AZD1480, AZD4877), and four were theoretical. The 3 + 3 method and 2-stage extended CRM with six skeletons were applied to identify the MTD, where the true MTD was considered as the largest dose where the probability of experiencing a dose limiting toxicity (DLT) is ≤33%.

Results

For every true dose-toxicity relationship, the CRM selected the MTD that matched the true MTD in a higher proportion of studies compared to the 3 + 3 method. The CRM overestimated the MTD in a higher proportion of simulations compared to the 3 + 3 method.

The proportion of studies where the correct MTD was selected varied considerably between skeletons. For some true dose-toxicity relationships, some skeletons identified the true MTD in a higher proportion of scenarios compared to the skeleton that matched the true dose-toxicity relationship.

Conclusion

Through simulation, the CRM generally outperformed the 3 + 3 method for the clinical and theoretical true dose-toxicity relationships. It was observed that accurate estimates of the true skeleton do not always outperform a generic skeleton, therefore the application of wide confidence intervals may enable a generic skeleton to be used. Further work is needed to determine the optimum skeleton.
Multiple anthropometric and nutritional deficiencies in young children in Ethiopia: a multi-level analysis based on a nationally representative data
Authors: Geda Nigatu Regassa , Feng Cindy Xin , Henry Carol J. , Lepnurm Rein , Janzen Bonnie , Whiting Susan J.
Published: January 2021

Background

In Ethiopia, child undernutrition and anemia are major public health concerns, resulting in increased childhood morbidity and mortality. Despite progress made to reduce the prevalence of malnutrition (especially stunting) from 50% in 2000 to 38% in 2016, little is known about the magnitude and risk factors for concurrent nutritional deficiencies in Ethiopia.

Methods

Analysis for this study was based on a total sample of 9218 children aged 6–59 months drawn from the Ethiopian Demographic and Health Survey (EDHS) conducted in the year 2016. The study used two outcome variables: Multiple nutrition deficit index formed by combining stunting, underweight, wasting and anemia status; and a concurrent stunting and anemia (CAS) index. Two mixed effect regression models, Poisson and Logistic, were used to identify the key risk factors of the two outcome variables, respectively.

Results

The proportion of children with stunting (length-for-age), underweight (weight-for-age) and wasting children (weight-for-length) was 38%, 25.2% and 9.4%, respectively. About 58% of the children had anemia. The prevalence of children with concurrent stunting and anemia children was 24.8%. Our results showed that the risks of multiple nutritional problems were determined by a range of individual, household and behavioral factors including: sex of the child, age of the child, birth order, parity, parental education, religion, household wealth index and type of family structure. The proximate variables (hygiene and sanitation score, feeding practice, and child health service utilization score) were also found to exert a strong influence on the risk of multiple nutritional deficiencies. The likelihood of co-occurrence of stunting and anemia was determined by certain individual and household factors, including sex of the child, age of the child, maternal education, household asset based wealth, religion and household hygiene and sanitation.

Conclusions

This study underscores the importance of improving parental education, household wealth, hygiene and sanitation conditions, promoting feeding practice and child health service utilization. Also, any nutrition sensitive and specific intervention should consider a child’s characteristics such as his/her age, gender and birth order.
Genetic susceptibility of opioid receptor genes polymorphism to drug addiction: A candidate-gene association study
Authors: AL-Eitan Laith N. , Rababa’h Doaa M. , Alghamdi Mansour A.
Published: January 2021

Background

Like other complex diseases including drug addiction, genetic factors can interfere with the disease. In this study, three opioid genes (OPRM1, OPRD1, and OPRK1) were examined for an association with drug addiction among Jordanian males.

Methods

The study involved 498 addicts, in addition to 496 healthy controls and all from Arab descent.

Results

The findings in this study showed that rs1799971 of the OPRM1 gene was in association with drug addiction for both alleles and genotypes with P-values = 0.002 and 0.01, respectively. In addition, a significant association between the dominant model (A/A vs G/A-G/G) of rs1799971 (OPRM1) and drug addiction (P-value = 0.003, OR = 1.59 (1.17–2.15)) was detected. Moreover, a genetic haplotype (AGGGCGACCCC) of theOPRM1 gene revealed a significant association with drug addiction (P-value = 0.01, OR = 1.56 (1.15–2.12)). We also found that the age of addicts, smoking, and marital status with genetic variants within OPRM1, OPRD1, and OPRK1 genes may be implicated in drug addiction risk.

Conclusion

We propose that rs1799971 of the OPRM1gene is a genetic risk factor for drug addiction among Jordanian males.
The potential role of network-oriented interventions for survivors of sexual and gender-based violence among asylum seekers in Belgium
Authors: Ogbe Emilomo , Jbour Alaa , Rahbari Ladan , Unnithan Maya , Degomme Olivier
Published: January 2021

Background

Social support and social network members have been identified as an important factor in mitigating the effects of sexual and gender-based violence (SGBV) and improving the coping process for many survivors. Network oriented strategies have been advocated for among domestic violence survivors, as they help build on improving social support and addressing factors that alleviate repeat victimization. There are opportunities to implement such strategies among asylum seekers who are survivors of SGBV in asylum centres, however, this has not been fully explored. This study sought to identify key strategies and opportunities for developing peer-led and network-oriented strategies for mitigating the effects of SGBV among asylum seekers at these centres.

Methods

Twenty-seven interviews, were conducted with service providers (n = 14) / asylum seekers (n = 13) at three asylum centres in Belgium. A theoretical model developed by the research team from a literature review and discussions with experts and stakeholders, was used as a theoretical framework to analyse the data. An abduction approach with qualitative content analysis was used by the two researchers to analyse the data. Data triangulation was done with findings from observations at these centres over a period of a year.

Results

Many of the asylum seekers presented with PTSD or psychosomatic symptoms, because of different forms of SGBV, including intimate partner violence, or other trauma experienced during migration. Peer and family support were very influential in mitigating the effects and social costs of violence among the asylum seekers by providing emotional and material support. Social assistants were viewed as an information resource that was essential for most of the asylum seekers. Peer-peer support was identified as a potential tool for mitigating the effects of SGBV.

Conclusion

Interventions involving asylum seekers and members of their network (especially peers), have the potential for improving physical and mental health outcomes of asylum seekers who are SGBV survivors.
Knowledge, attitude and perception of Pakistanis towards COVID-19; a large cross-sectional survey
Authors: Ladiwala Zoya Fatima Rizwan , Dhillon Rubaid Azhar , Zahid Ibrahim , Irfan Omar , Khan Muhammad Sharjeel , Awan Safia , Khan Javaid Ahmad
Published: January 2021

Background

The Novel Coronavirus Disease (COVID-19) has created havoc globally as countries worldwide struggle to combat this pandemic. Since prevention and social isolation are known to be the only ways to prevent the spread of COVID-19, this has created challenges among the lower-middle income countries (LMIC) including Pakistan, as it battles between an under-resourced healthcare, an economic shutdown, and widespread myths and misconceptions. Therefore, a study was conducted to evaluate the knowledge, attitude and perceptions regarding COVID-19 as public understanding is vital to help facilitate the control of this outbreak.

Methods

A pre-validated online questionnaire was distributed among the general population of Pakistan from 1st to 12th June 2020. Descriptive statistics were analyzed using SPSS v25. Adequate knowledge was assigned as a score of > 4 (range: 0–8) and good perception as a score of > 3 (range: 0–5). Chi-square test was used to determine the significance of difference in knowledge and perception of COVID-19 with socio-demographic characteristics. Logistic regression analysis was run to identify factors associated with adequate knowledge and perception.P < 0.05 was considered as significant.

Results

A total of 1200 respondents participated in this study with a wide range of age groups and education. Majority of the respondents had adequate knowledge (93.3%) with a mean score of 6.59 ± 1.35, and good perception (85.6%) with a mean score of 4.29 ± 0.82. Significant differences in knowledge and perception were observed among genders, age groups, education and between students and employees in the healthcare and non-healthcare department. A multivariate analysis revealed a higher educational status and female gender to be significant predictors of adequate knowledge and perception.

Conclusions

Albeit the surge of COVID-19 cases in Pakistan, the participants demonstrated an overall adequate knowledge and good perception towards COVID-19. There is a need to follow the preventive protocols and dissemination of correct information through conducting educational interventions that target safe health practices and provide appropriate information on this infection.
Parasitosis of the vertebral canal mimicking lumbar intervertebral disc herniation: a case report
Authors: Tyrakowski Marcin , Kwiatkowska Magdalena , Czubak-Wrzosek Maria , Czubak Jarosław
Published: January 2021

Study design

Case report.

Background

It is a case of dracunculiasis of the spine mimicking lumbar intervertebral disc herniation.

Case presentation

A 57 year-old Caucasian male was admitted to the hospital because of the left L5 radiculopathy lasting for 2 months. The pain in the left lower limb was associated with muscle weakness on dorsal flexion of the foot, paresthesia of the dorsal aspect of the foot and tingling in the big toe. Neurological examination revealed: muscle weakness on dorsal flexion of the foot, impaired light touch and pin prick test on the dorsal aspect of the foot and positive Lasègue’s sign. Magnetic resonance imaging (MRI) examination revealed L4-L5 intervertebral disc herniation with sequester compressing the left L5 nerve root. The open L4-L5 left side discectomy was performed. During the sequester evacuation 3 pieces of nematodes were removed and preserved in 10% of formaldehyde solution. After the surgery the patient was pain free with normal neurological examination. The diagnosis of dracunculiasis was based on the morphology of the nematode and on exclusion of the other parasites. DM infestation could not be confirmed with molecular testing that was impaired by the formaldehyde.

Conclusions

Parasite infestation should be considered even in cases with obvious MRI of lumbar intervertebral disc herniation. If a nematode was found accidentally during any surgery it should be preserved in a 0.9% saline, not in formaldehyde, not to disturb the molecular tests.
Will virtual multidisciplinary team meetings become the norm for musculoskeletal oncology care following the COVID-19 pandemic? - experience from a tertiary sarcoma centre
Authors: Rajasekaran Raja Bhaskara , Whitwell Duncan , Cosker Thomas D. A. , Gibbons Christopher L. M. H. , Carr Andrew
Published: January 2021

Background

Like with all cancers, multidisciplinary team (MDT) meetings are the norm in bone and soft tissue tumour (BST) management too. Problem in attendance of specialists due to geographical location is the one of the key barriers to effective functioning of MDTs. To overcome this problem, virtual MDTs involving videoconferencing or telemedicine have been proposed, but however this has been seldom used and tested. The COVID-19 pandemic forced the implementation of virtual MDTs in the Oxford sarcoma service in order to maintain normal service provision. We conducted a survey among the participants to evaluate its efficacy.

Methods

An online questionnaire comprising of 24 questions organised into 4 sections was circulated among all participants of the MDT after completion of 8 virtual MDTs. Opinions were sought comparing virtual MDTs to the conventional face-to-face MDTs on various aspects. A total of 36 responses were received and were evaluated.

Results

72.8% were satisfied with the depth of discussion in virtual MDTs and 83.3% felt that the decision-making in diagnosis had not changed following the switch from face-to-face MDTs. About 86% reported to have all essential patient data was available to make decisions and 88.9% were satisfied with the time for discussion of patient issues over virtual platform. Three-fourths of the participants were satisfied (36.1% - highly satisfied; 38.9% - moderately satisfied) with virtual MDTs and 55.6% of them were happy to attend MDTs only by the virtual platform in the future. Regarding future, 77.8% of the participants opined that virtual MDTs would be the future of cancer care and an overwhelming majority (91.7%) felt that the present exercise would serve as a precursor to global MDTs involving specialists from abroad in the future.

Conclusion

Our study shows that the forced switch to virtual MDTs in sarcoma care following the unprecedented COVID-19 pandemic to be a viable and effective alternative to conventional face-to-face MDTs. With effective and efficient software in place, virtual MDTs would also facilitate in forming extended MDTs in seeking opinions on complex cases from specialists abroad and can expand cancer care globally.
Experiences of nurses educated outside the European Union of a Swedish bridging program and the program’s role in their integration into the nursing profession: a qualitative interview study
Authors: Hadziabdic Emina , Marekovic Anna-Maria Sarstrand , Salomonsson Johanna , Heikkilä Kristiina
Published: January 2021

Background

Countries all over the world are experiencing a shortage of registered nurses (RNs). Therefore, some countries, including Sweden, have tried to solve this by recruiting internationally educated nurses (IENs). Countries offer bridging programs as educational support to qualify IENs for nursing work in the destination country. However, there is little research on IENs’ experiences of bridging programs in European countries and how these programs facilitate their integration into the world of work and their new society. The aim of this study is to explore the experiences of nurses, originally educated outside the EU (European Union)/EES, of the Swedish bridging program and of the program’s role in facilitating their integration into the nursing profession in Sweden.

Methods

A qualitative descriptive design was used to explore the topic based on 11 informants’ perspectives and experiences. Purposive sampling was used to recruit participants at one university in Sweden. Data were collected by individual interviews using a semi- structured interview guide during the year 2019 and were analysed using an interpretative thematic approach.

Results

Two main themes emerged from the analysis: 1) Return to nursing, and 2) The bridging program as a tool for transition to nursing in Sweden. The first theme includes conditions and experiences such as personal motivation and determination, and support from others that the participants described as important in order to achieve the goal of re-establishing themselves as registered nurses in Sweden. Furthermore, the second theme describes the participants’ experiences of the bridging program as mostly positive because it led to new learning and achievements that were valuable for the transition to nursing in Sweden; however, the participants also emphasised the challenges of their transition into the nursing profession, which were related to instances of misrecognition of their professional competence and the uncertain outcome of the program.

Conclusions

This study found that the bridging program facilitated integration into the nursing profession for nurses educated outside the EU/EES, especially knowledge gained in clinical-based training. Thus, it is important to recognise and value the IENs’ experience and previous knowledge and training when developing the bridging program’s curriculum.
Hemolysis at low blood flow rates: in-vitro and in-silico evaluation of a centrifugal blood pump
Authors: Schöps Malte , Groß-Hardt Sascha H. , Schmitz-Rode Thomas , Steinseifer Ulrich , Brodie Daniel , Clauser Johanna C. , Karagiannidis Christian
Published: January 2021

Background

Treating severe forms of the acute respiratory distress syndrome and cardiac failure, extracorporeal membrane oxygenation (ECMO) has become an established therapeutic option. Neonatal or pediatric patients receiving ECMO, and patients undergoing extracorporeal CO2removal (ECCO2R) represent low-flow applications of the technology, requiring lower blood flow than conventional ECMO. Centrifugal blood pumps as a core element of modern ECMO therapy present favorable operating characteristics in the high blood flow range (4 L/min–8 L/min). However, during low-flow applications in the range of 0.5 L/min–2 L/min, adverse events such as increased hemolysis, platelet activation and bleeding complications are reported frequently.

Methods

In this study, the hemolysis of the centrifugal pump DP3 is evaluated both in vitro and in silico, comparing the low-flow operation at 1 L/min to the high-flow operation at 4 L/min.

Results

Increased hemolysis occurs at low-flow, both in vitro and in silico. The in-vitro experiments present a sixfold higher relative increased hemolysis at low-flow. Compared to high-flow operation, a more than 3.5-fold increase in blood recirculation within the pump head can be observed in the low-flow range in silico.

Conclusions

This study highlights the underappreciated hemolysis in centrifugal pumps within the low-flow range, i.e. during pediatric ECMO or ECCO2R treatment. The in-vitro results of hemolysis and the in-silico computational fluid dynamic simulations of flow paths within the pumps raise awareness about blood damage that occurs when using centrifugal pumps at low-flow operating points. These findings underline the urgent need for a specific pump optimized for low-flow treatment. Due to the inherent problems of available centrifugal pumps in the low-flow range, clinicians should use the current centrifugal pumps with caution, alternatively other pumping principles such as positive displacement pumps may be discussed in the future.
P2X7R-mediated autophagic impairment contributes to central sensitization in a chronic migraine model with recurrent nitroglycerin stimulation in mice
Authors: Jiang Li , Zhang Yixin , Jing Feng , Long Ting , Qin Guangcheng , Zhang Dunke , Chen Lixue , Zhou Jiying
Published: January 2021

Background

Central sensitization is an important pathophysiological mechanism of chronic migraine (CM). According to our previous studies, microglial activation and subsequent inflammation in the trigeminal nucleus caudalis (TNC) contribute to the central sensitization. The P2X7 receptor (P2X7R) is a purinergic receptor expressed in microglia and participates in central sensitization in chronic pain, but its role in CM is unclear. Numerous studies have shown that P2X7R regulates the level of autophagy and that autophagy affects the microglial activation and inflammation. Recently, autophagy has been shown to be involved in neuropathic pain, but there is no information about autophagy in CM. Therefore, the current study investigated the role of P2X7R in CM and its underlying mechanism, focusing on autophagy regulation.

Methods

The CM model was established by repeated intraperitoneal injection of nitroglycerin (NTG) in mice. A Von Frey filament and radiant heat were used to assess the mechanical and thermal hypersensitivity. Western blotting and immunofluorescence assays were performed to detect the expression of P2X7R, autophagy-related proteins, and the cellular localization of P2X7R. To determine the role of P2X7R and autophagy in CM, we detected the effects of the autophagy inducer, rapamycin (RAPA) and P2X7R antagonist, Brilliant Blue G (BBG), on pain behavior and the expression of calcitonin gene-related peptide (CGRP) and c-fos. In addition, the effect of RAPA and BBG on microglial activation and subsequent inflammation were investigated.

Results

The expression of P2X7R was increased and was mainly colocalized with microglia in the TNC following recurrent NTG administration. The autophagic flux was blocked in CM, which was characterized by upregulated LC3-II, and accumulated autophagy substrate protein, p62. RAPA significantly improved the basal rather than acute hyperalgesia. BBG alleviated both basal and acute hyperalgesia. BBG activated the level of autophagic flux. RAPA and BBG inhibited the activation of microglia, limited the inflammatory response, and reduced the expression of CGRP and c-fos.

Conclusions

Our results demonstrate the dysfunction of the autophagic process in CM. Activated autophagy may have a preventive effect on migraine chronification. P2X7R contributes to central sensitization through mediating autophagy regulation and might become a potential target for CM.
Gut microbiota as the key controllers of “healthy” aging of elderly people
Authors: Ragonnaud Emeline , Biragyn Arya
Published: January 2021

Extrinsic factors, such as lifestyle and diet, are shown to be essential in the control of human healthy aging, and thus, longevity. They do so by targeting at least in part the gut microbiome, a collection of commensal microorganisms (microbiota), which colonize the intestinal tract starting after birth, and is established by the age of three. The composition and abundance of individual microbiota appears to continue to change until adulthood, presumably reflecting lifestyle and geographic, racial, and individual differences. Although most of these changes appear to be harmless, a major shift in their composition in the gut (dysbiosis) can trigger harmful local and systemic inflammation. Recent reports indicate that dysbiosis is increased in aging and that the gut microbiota of elderly people is enriched in pro-inflammatory commensals at the expense of beneficial microbes. The clinical consequence of this change remains confusing due to contradictory reports and a high degree of variability of human microbiota and methodologies used. Here, we present the authors’ thoughts that underscore dysbiosis as a primary cause of aging-associated morbidities, and thus, premature death of elderly people. We provide evidence that the dysbiosis triggers a chain of pathological and inflammatory events. Examples include alteration of levels of microbiota-affected metabolites, impaired function and integrity of the gastrointestinal tract, and increased gut leakiness. All of these enhance systemic inflammation, which when associated with aging is termed inflammaging, and result in consequent aging-associated pathologies.
The clinicopathological relevance of uniform CD56 expression in anaplastic large cell lymphoma: a retrospective analysis of 18 cases
Authors: Yu Bao-Hua , Zhang Yan , Xue Tian , Shui Ruo-Hong , Lu Hong-Fen , Zhou Xiao-Yan , Zhu Xiong-Zeng , Li Xiao-Qiu
Published: January 2021

Background

Anaplastic large cell lymphoma (ALCL) with uniform CD56 expression is a rare condition, that has been described in limited literature, and its clinicopathological features have not yet been well illustrated. The aim of our study was to fully investigate the clinical, histological, immunohistochemical and molecular features of CD56+ ALCL.

Methods

The clinical and histological characteristics of CD56+ ALCL cases were retrospectively evaluated. The immunohistochemical phenotype, status of Epstein-Barr virus (EBV) and T-cell receptor (TCR) gene rearrangement were examined. Overall survival was also analyzed.

Results

Eighteen (5.8%) cases with diffuse CD56 expression were identified out of 313 archived ALCL cases with CD56 test. CD56 expression was significantly higher in ALK+ systemic ALCLs (sALCLs) (13/64, 20.3%) than in ALK- sALCLs (3/101, 3.0%) (p < 0.001) as well as primary cutaneous ALCLs (2/148, 1.4%) (p < 0.001). Regarding the CD56+ ALK+ sALCLs, the median age was 20 years (range, 8–60 years) with a male-to-female ratio of 2.3:1, and these cases more frequently affected extranodal sites (11/38, 28.9%) rather than lymph nodes (2/26, 7.7%) (p = 0.038). Eleven (84.6%) cases presented with stage I-II diseases, which was significantly more than their CD56- ALK+ counterparts (45.5%) (p = 0.015). Histologically, 2 ALK+ cases were of small cell variant and all the others displayed characteristic morphology of classic ALCL. Regarding the immunophenotype, both CD30 and CD56 were diffusely positive in all cases. CD3, CD43, anaplastic lymphoma kinase-1 (ALK1), TIA-1, EMA expression was observed in 30.8% (4/13), 90.9% (10/11), 100% (13/13), 100% (9/9), and 80.0% (8/10) cases, respectively. EBV infection was consistently absent. Monoclonal TCR gene rearrangement was found in 100% (5/5) of investigated ALK+ cases. Chemotherapy with a CHOP regimen was most frequently employed. The 3-year overall survival (OS) rate for CD56+ ALK+ patients was 92.0%, compared with 73.0% for their CD56- counterparts, but there was no significant difference in OS between the two groups (p = 0.264).

Conclusions

Uniform CD56 expression is an unexpected condition in ALCL. Of ALK+ ALCLs, CD56 expression correlated with a high frequency of early stage and an extranodal predominance. It is of great importance to raise awareness of this condition and familiarity with its characteristic features to avoid diagnostic and therapeutic pitfalls. Further investigations are warranted for a better understanding of this unusual phenotype and the significance of CD56 expression in ALCL.
Taxonomic and phylogenetic composition show biotic resistance to exotic invasion in acid seep springs
Authors: Barfknecht David F. , Gibson David J.
Published: January 2021

Background

Few studies have incorporated the evolutionary insights provided by analysis of phylogenetic structure along with community composition to assess the effects of exotic invasion on freshwater wetlands. Here, we assess the taxonomic and phylogenetic relationships among acid seep springs to investigate the potential homogenization or resistance of communities due to invasion of an exotic grass.

Results

Multivariate community analyses indicated differences in community and phylogenetic composition and dispersion among acid seep springs, associated with gradients in soil moisture, canopy cover, and phylogenetic diversity. By contrast, univariate analyses showed differences in taxonomic diversity but not phylogenetic diversity among acid seep springs.

Conclusions

Despite exotic invasion, individual acid seep springs remained taxonomically and phylogenetically distinct from each other. Taxonomic and phylogenetic diversity metrics revealed different aspects of composition, reinforcing the importance of including both in analyses of plant communities for understanding community assembly following exotic invasion and for management purposes. Within acid seep springs, taxonomic and phylogenetic composition appear to be driven more through environmental filtering by light and moisture than by the competitive effects following invasion of an exotic grass in support of Elton’s biotic resistance hypothesis.
First records of the megamouth shark Megachasma pelagios (Taylor, Compagno & Struhsaker, 1983) as bycatch in Peruvian small-scale net fisheries
Authors: Acuña-Perales Nicolas , Córdova-Zavaleta Francisco , Alfaro-Shigueto Joanna , Mangel Jeffrey C.
Published: January 2021

Between October 2018 and October 2019, seven megamouth sharks were reported as bycatch in the small-scale surface and midwater gillnet fisheries operating from the ports of Mancora and Salaverry in northern Peru. Here we present details of these specimens and bycatch events that represent the first documented records of this species in Peruvian waters, confirming their occurrence as fisheries bycatch. We recommend continued monitoring programs in small-scale fisheries toward assessing the extent of this species’ occurrence in Peru and its vulnerability to fishery interactions.
θ-deformed multi-dimensional fermion algebras
Authors: Dobie A. Peker
Published: January 2021

The conventional d-dimensional fermion algebra does not have a Hopf algebra structure. It is shown that this algebra can be deformed by introducing angular elements and by combining the commutative sine-cosine algebra with the Clifford algebra so that the resulting d-dimensional fermion algebra is a Hopf algebra.
Gaussian closure scheme in the quasi-linkage equilibrium regime of evolving genome populations
Authors: Mauri E. , Cocco S. , Monasson R.
Published: January 2021

Describing the evolution of a population of genomes evolving in a complex fitness landscape is generally very hard. We here introduce an approximate Gaussian closure scheme to characterize analytically the statistics of a genomic population in the so-called quasi-linkage equilibrium (QLE) regime, applicable to generic values of the rates of mutation or recombination and fitness functions. The Gaussian approximation is illustrated on a short-range fitness landscape with two far away and competing maxima. It unveils the existence of a phase transition from a broad to a polarized distribution of genomes as the strength of epistatic couplings is increased, characterized by slow coarsening dynamics of competing allele domains. Results of the closure scheme are corroborated by numerical simulations.
A segurança jurídica dos atos administrativos e a objetivação das demandas refletidas no art. 30 da nova LINDB
Authors: Ribeiro Cardoso Henrique , Barretto Dória Davi
Published: January 2021

A trajetória da legislação brasileira demonstra a intenção do legislador em garantir, na ordem prática, o princípio da segurança jurídica. O problema analisado neste artigo parte da constatação de que esse princípio positivado na Constituição Federal não estava devidamente disciplinado e regulamentado na seara administrativa. Como hipótese, busca-se examinar as recentes inovações na LINDB que demonstram a necessidade de consagração do mencionado princípio. Atento à nova conformação daadministração pública, o legislador acrescentou o art. 30 à LINDB, com a finalidade de assegurar uma maior observância da segurança jurídica na aplicação da norma. O objetivo deste artigo é elucidar o conceito de segurança jurídica que passa a ter densidade normativa e conteúdo próprios nesta inovação legislativa.
Topical Systematic and Synthetic Literature Review Regarding Men Sexual Dysfunctions after Spinal Cord Injury
Authors: SERBAN Diana-Elena , DAIA Cristina Octaviana , NEGOESCU CHEREGI Ioana , CIOBANU Vlad , ONOSE Liliana , POPESCU Cristina , ONOSE Gelu
Published: January 2021

Introduction Spinal cord injury (SCI) is a life-altering event usually associated with loss of motor and sensory, as well as with bladder, bowel and sexual, functions impairment. Recovering sexual function is one of the most important function tightly coupled with the life quality. In this respect, in the related literature can be found data regarding mainly: diagnosis/evaluation issues therapeutic/assistive-rehabilitative interventions (including connected to fertility troubles) and of psychological and or educational specific counseling, kind.
Materials and methods.This paper presents a current systematic (of Preferred Reporting Items for Systematic Reviews and Meta-Analyses – PRISMA – type) and synthetic literature review on sexual dysfunctions and respected available management options in male subjects with SCI, using the following search keywords/ combinations of key words: “men”, “sexual dysfunction”/ “fertility” / “erectile dysfunction”/ “ejaculatory problems” / “sexual disorder“, “spinal cord injury”, “paraplegia”/ ”tetraplegia” /“paraplegic”/ ”tetraplegic”, “management”/ “treatment”, by interrogating international renown data bases: NCBI/PubMed, NCBI/PMC, Elsevier, PEDro and respectively, ISI Web of Knowledge/Science – to check whether the selected articles are published in ISI indexed journals – considering publications from January 2009 to June 2019, written in English, open access articles and being “fair”/“high” quality on our PEDro inspired, customized quality classification of the selected papers – the basic criterion, being the weighted citations number per year.
Results. We have found initially 647 articles and eventually, after accomplishing the PRISMA stages (without meta-analysis), we have selected 16 articles matching all the above mentioned quest method’s requests (see further the figure representing our PRISMA type completed flow-diagram), covering (together with knowledge acquired from extra bibliographic resources, too).
Conclusions. Sexual disfunctions after SCI are complex and strongly add to the severe and multimodal disability the affected people – in the case of our work: men – experience. Therefore, they worth being fathomed and periodically reappraised.

Keywords: Spinal Cord injury (SCI), men sexual dysfunctions, systematic literature review, rehabilitation,