bluuebunny/crossref_metadata_2025 huggingface dataset:
PlotXpress, a webtool for normalization and visualization of reporter expression data
Authors: Brandorff Elias , Galland Marc , Goedhart Joachim
Published: July 2021

In molecular cell biology, reporter assays are frequently used to investigate gene expression levels. Reporter assays employ a gene that encodes a light-emitting protein, of which the luminescence is quantified as a proxy of gene expression. Commercial parties provide reporter assay kits that include protocols and specialized detection machinery. However, downstream analysis of the output data and their presentation are not standardized. We have developed plotXpress to fill this gap, providing a free, open-source platform for the semi-automated analysis and standardized visualisation of experimental gene reporter data. Users can upload raw luminescence data acquired from a reporter gene assay with an internal control. In plotXpress, the data is corrected for sample variation with the internal control and the average for each condition is calculated. When a reference condition is selected the fold change is calculated for all other conditions, based on the selected reference. The results are shown as dot plots with a statistical summary, which can be adjusted to create publication-grade plots without requiring coding skills. Altogether, plotXpress is an open-source, low-threshold, web-based tool, that promotes a standardized and reproducible analysis while providing an appealing visualization of reporter data. The webtool can be accessed at: https://huygens.science.uva.nl/PlotXpress/

Abstract Figure
Identifying cellular-to-phenotype associations by elucidating hierarchical relationships in high-dimensional cytometry data
Authors: Chan Adam , Jiang Wei , Blyth Emily , Yang Jean , Patrick Ellis
Published: July 2021

High-throughput single cell technologies hold the promise of discovering novel cellular relationships with disease. However, analytical workflows constructed for these technologies to associate cell proportions with disease often employ unsupervised clustering techniques that overlook the valuable hierarchical structures that have been used to define cell types. We present treekoR, a framework that empirically recapitulates these structures, facilitating multiple quantifications and comparisons of cell type proportions. Our results from twelve case studies reinforce the importance of quantifying proportions relative to parent populations in the analyses of cytometry data — as failing to do so can lead to missing important biological insights.
Pseudomonas syringae effector AvrE associates with plant membrane nanodomains and binds phosphatidylinositides in vitro
Authors: Xin Xiu-Fang , Kinch Lisa , Cai Boying , Paasch Bradley C. , Kvitko Brian , Grishin Nick V. , He Sheng Yang
Published: July 2021

Bacterial phytopathogens deliver effector proteins into host cells as key virulence weapons to cause disease. Extensive studies revealed diverse functions and biochemical properties of different effector proteins from pathogens. In this study, we show that the Pseudomonas syringae effector AvrE, the founding member of a broadly conserved and pathologically important bacterial effector family, binds to phosphatidylinositides (PIPs) in vitro and shares some properties with eukaryotic PROPPINs (β-propellers that bind polyphosphoinositides). In planta pull down experiments with transgenic Arabidopsis plants expressing AvrE revealed that AvrE is associated with several plant proteins including plasma membrane lipid-raft proteins. These results shed new light on the properties of a bacterial effector that is crucial for bacterial virulence in plants.
Structure of a germline-like human antibody defines a neutralizing epitope on the SARS-CoV-2 spike NTD
Authors: Altomare Clara G. , Adelsberg Daniel C. , Carreno Juan Manuel , Sapse Iden A. , Amanat Fatima , Ellebedy Ali H. , Simon Viviana , Krammer Florian , Bajic Goran
Published: July 2021

Structural characterization of infection- and vaccination-elicited antibodies in complex with antigen provides insight into the evolutionary arms race between the host and the pathogen and informs rational vaccine immunogen design. We isolated a germline-like monoclonal antibody (mAb) from plasmablasts activated upon mRNA vaccination against SARS-CoV-2 and determined its structure in complex with the spike glycoprotein by cryo-EM. We show that the mAb engages a previously uncharacterized neutralizing epitope on the spike N-terminal domain (NTD). The high-resolution structure reveals details of the intermolecular interactions and shows that the mAb inserts its HCDR3 loop into a hydrophobic NTD cavity previously shown to bind a heme metabolite, biliverdin. We demonstrate direct competition with biliverdin and that - because of the conserved nature of the epitope – the mAb maintains binding to viral variants B.1.1.7 and B.1.351. Our study illustrates the feasibility of targeting the NTD to achieve broad neutralization against SARS-CoV-2 variants.
Structural modeling of the hERG potassium channel and associated drug interactions
Authors: Maly Jan , Emigh Aiyana M. , DeMarco Kevin R. , Furutani Kazuharu , Sack Jon T. , Clancy Colleen E. , Vorobyov Igor , Yarov-Yarovoy Vladimir
Published: July 2021

The voltage-gated potassium channel, KV11.1, encoded by the humanEther-à-go-go- Related Gene (hERG) is expressed in cardiac myocytes, where it is crucial for the membrane repolarization of the action potential. Gating of hERG channel is characterized by rapid, voltage-dependent, C-type inactivation, which blocks ion conduction and is suggested to involve constriction of the selectivity filter. Mutations S620T and S641A/T within the selectivity filter region of hERG have been shown to alter the voltage- dependence of channel inactivation. Because hERG channel blockade is implicated in drug-induced arrhythmias associated with both the open and inactivated states, we used Rosetta to simulate effects of hERG S620T and S641A/T mutations to elucidate conformational changes associated with hERG channel inactivation and differences in drug binding between the two states. Rosetta modeling of the S641A fast-inactivating mutation revealed a lateral shift of F627 side chain in the selectivity filter into the central channel axis along the ion conduction pathway and formation of four lateral fenestrations in the pore. Rosetta modeling of the non-inactivating mutations S620T and S641T suggested a potential molecular mechanism preventing F627 side chain from shifting into the ion conduction pathway during the proposed inactivation process. Furthermore, we used Rosetta docking to explore the binding mechanism of highly selective and potent hERG blockers - dofetilide, terfenadine, and E4031. Our structural modeling correlate well with existing experimental evidence involving interactions of these drugs with key hERG residues Y652 and F656 inside the pore and reveal potential ligand binding interactions within fenestration region in an inactivated state.

Significance Statement

Computational models of hERG potassium channel provide structural insights into an inactivated state and associated drug interactions. Our computational approach will be useful to study ion channel modulation by small molecules.
Recruitment of Scc2/4 to double strand breaks depends on yH2A and DNA end resection
Authors: Scherzer Martin , Giordano Fosco , Ferran Maria Solé , Ström Lena
Published: July 2021

Homologous recombination (HR) enables cells to overcome the threat of DNA double strand breaks (DSB), allowing for repair without the loss of genetic information. Central to the HR repair process is the de novo loading of Cohesin around a DSB by its loader complex Scc2/4. Although Cohesin’s accumulation at these sites has been well studied, the prerequisites leading to Scc2/4 recruitment during the repair process are still elusive. Here we investigate which factors are required for recruitment of Scc2 around DSBs in Saccharomyces cerevisiae. To address this question, we combined ChIP-qPCR with a GAL-inducible HO-endonuclease system to generate a site specific DSB in vivo. We find that Scc2 recruitment relies on yH2A and Tel1, but as opposed to Cohesin, not on Mec1. We further demonstrate that binding of Scc2 depends on and coincides with DNA end resection. Although affected by the impact on resection, this recruitment of Scc2 is not directly facilitated by the RSC, SWR1 or INO80 complexes. Our results shed light on the intricate DSB repair cascade leading to the recruitment of Scc2/4 and the subsequent loading of Cohesin.
tert-Butyl 3-amino-5-bromo-1H-indazole-1-carboxylate
Authors: Thiruvalluvar Aravazhi Amalan , Kusanur Raviraj , Sridharan Makuteswaran
Published: July 2021

In the title compound, C12H14BrN3O2, the pyrazole and benzene rings are nearly co-planar with a dihedral angle between the rings of 2.36 (5)°. In the crystal, inversion dimers linked by pairwise N—H...N hydrogen bonds generate R
2
2(8) loops. The dimers are linked into a three-dimensional network by weak aromatic π–π stacking interactions [centroid–centroid separation = 3.7394 (6) Å] and C—H...O and C—H...Br hydrogen bonds.
Construct validation of leaders’ motivating language in the context of the hotel industries of Bangladesh and Malaysia
Authors: Rabiul Md Karim , Fee Yean Tan , Patwary Ataul Karim , Mohamed Ahmad Edwin , Hilman Haim
Published: July 2021

Purpose

This study aims to validate the motivating language scale developed by Mayfield et al. (1995) in the context of the hotel industries of Bangladesh and Malaysia.

Design/methodology/approach

For Study 1, data were collected from employees (lower and mid-level) of three to five-star hotels in Bangladesh. Customer-contact employees working in four- and five-star hotels in Malaysia participated in Study 2. Both studies featured a cross-sectional survey design.

Findings

Factor analysis revealed that both samples provided three-factor solutions for the motivating language scale in both countries. Convergent, discriminant and nomological validity were assessed by testing with outcome variables of motivating language.

Research limitations/implications

Future studies may use all three versions of the scale (Bengali, Malay and English) to collect data, as all three have been validated.

Originality/value

By validating the Bengali and Malay versions of the motivating language scale, this study contributes to the leadership language literature, specifically that related to hotel industries in developing contexts.
A new operation strategy of PEV parking and hydro storage in smart grid environment
Authors: Nazari Mohammad Esmaeil , Zarrini Farahmand Mahsa
Published: July 2021

Purpose

The purpose of this study is to solve the optimal operation strategy problem of plug-in electric vehicles (PEV) parking as a demand response (DR) program and hydro storage as an energy storage system in a smart grid environment using a heuristic algorithm.

Design/methodology/approach

Studying the smart grid with DR, renewable energy resources and energy storage systems is necessary. To do this, the heuristic optimization algorithm is developed to solve the scheduling problem. This deterministic algorithm benefits from the definition of appropriate fitness functions.

Findings

For validation, it is shown that reduction of 1.1%–12.5% in pollution and 8.8%–34.8% in total cost are achieved, as compared with literature. Also, the suggested operation strategy of PEVs parking and hydro storage results in reducing the total cost by 6.21%.

Originality/value

DR programs such as PEV parking play a major role in smart grid developments. Also, energy storage systems such as hydro storage lead to better performance of distributed generations and lower costs and pollution by thermal units. However, based on the literature, the effects of PEV parking and hydro storage on smart grid operation strategy are not considered. Therefore, contributions of this study are: effects of hydro storage on the smart grid are considered, effects of PEV parking on the smart grid are considered, a heuristic algorithm is developed to solve operation strategy problem for PEV parking and hydro storage in a smart grid environment.
Health boards' governance of quality and risk: quality improvement agenda for the board
Authors: Avery Mark J. , Cripps Allan W. , Rogers Gary D.
Published: July 2021

Purpose

This study explores key governance, leadership and management activities that have impact on quality, risk and safety within Australian healthcare organisations.

Design/methodology/approach

Current non-executive directors (n = 12) of public and private health boards were interviewed about contemporary approaches to fiduciary and corporate responsibilities for quality assurance and improvement outcomes in the context of risk and safety management for patient care. Verbatim transcripts were subjected to thematic analysis triangulated with Leximancer-based text mining.

Findings

Boards operate in a strong legislative, healthcare standards and normative environment of quality and risk management. Support and influence that create a positive quality and risk management culture within the organisation, actions that disseminate quality and risk broadly and at depth for all levels, and implementation and sustained development of quality and risk systems that report on and contain risk were critical tasks for boards and their directors.

Practical implications

Findings from this study may provide health directors with key quality and risk management agenda points to expand or deepen the impact of governance around health facilities' quality and risk management.

Originality/value

This study has identified key governance activities and responsibilities where boards demonstrate that they add value in terms of potential improvement to hospital and health service quality care outcomes. The demonstrable influence identified makes an important contribution to our understanding of healthcare governance.
Fostering healthcare innovation: a qualitative study of dental implant product development by Thai university researchers
Authors: Sooampon Sutti , Pisarnturakit Pagaporn Pantuwadee , Sooampon Sireerat
Published: July 2021

Purpose

This study investigated the conditions required to foster healthcare innovation. Due to the limited research and development (R&D) capability of the Thai private sector, universities are increasingly expected to be emerging sources of healthcare products. However, the lack of experience in research commercialization requires that whether and how Thai university researchers can serve this mission be explored.

Design/methodology/approach

A promising dental implant product development project was investigated using the single-case research methodology. In-depth interviews were conducted with three key members involved in this innovative project. Questions concerning how a team of university researchers could embark on and overcome the obstacles encountered during their entrepreneurial project were asked. Based on the conceptual foundation of academic entrepreneurship, primary and additional secondary data were analyzed to acquire knowledge of academic entrepreneurship in healthcare in an emerging economy.

Findings

Healthcare inequality has generated entrepreneurial initiatives by healthcare researchers to develop low-cost dental implants. However, their efforts have been insufficient to progress to the commercialization stage. An informal relationship among socially oriented partners, including interdisciplinary experts, not-for-profit-oriented manufacturers and early adopters contributed to this project's success.

Originality/value

To foster healthcare innovation, the authors’ micro-level evidence of a socially oriented partnership on an informal basis indicates the need for inter-professional governance that maintains a cross-organizational environment between healthcare researchers and external parties. The future contributions to innovation management by healthcare professionals should be enhanced. A socially entrepreneurial model of healthcare innovation is suggested for further consideration as an enabling policy option for inter-professional partnership in emerging economies.
Influence of selection criteria on clients’ satisfaction with construction consultancy services in Nigeria
Authors: Ogbu Chukwuemeka Patrick , Imafidon Monday Omogiate
Published: July 2021

Purpose

To receive a satisfactory consultancy service, a construction client must first select suitable consultants. While numerous criteria for the selection of construction consultants have been suggested in the literature, their influence on client satisfaction has hardly been statistically established. This study aimed to reduce the criteria for the selection of construction consultants into a more manageable set of fewer factors and ascertain the influence of the factors on client satisfaction.

Design/methodology/approach

Data were gathered through a purposively administered cross-sectional survey of public sector clients in Nigeria. Analyzes were done using relative importance index, factor analysis and multiple regression analysis.

Findings

The results proved that the criteria for the selection of consultants can, in the order of decreasing influence on client satisfaction, be grouped into service delivery approach (SDA), relationship with the client, the caliber of personnel, firm reputation and firm certification. However, SDA is more influential on client satisfaction. All the factors were found to have significant statistical effects on clients’ overall satisfaction with consultancy services.

Originality/value

The study demonstrates the extent to which construction consultant selection criteria are relevant to client satisfaction. It shows that SDA is the most important predictor of clients’ satisfaction with consultancy services. The results are helpful for grouping consultant selection criteria in future studies, and in guiding clients on the weights to assign to consultant selection criteria during tender evaluation.
Access to non-violent pornography in a secure forensic hospital
Authors: Nally Thomas , Ireland Jane L. , McNeill Kimberley , Birch Philip , Ireland Carol A.
Published: July 2021

Purpose

The purpose of this paper is to explore non-violent pornography within secure hospital settings.

Design/methodology/approach

It includes a systematic review (n = 40 papers), followed by a qualitative study comprising semi-structured interviews (n = 24, 6 patients and 18 staff) and staff focus groups (n = 22 staff).

Findings

The systematic review identified six themes, as follows: pornography is inconsistently defined, pornography exposure can increase general aggression, pornography exposure may increase the risk for sexual aggression, pornography exposure can increase aggression supportive beliefs, pornography exposure impacts negatively on those with a violent predisposition and pornography is educational for men not identifying as heterosexual. The semi-structured interviews and focus groups revealed four themes as follows: staff members hold diverse beliefs about pornographic material, pornography is difficult to obtain and use for patients who do not identify as heterosexual, pornography is used for specific functions, and frequent exposure to pornography can have negative effects for staff members.

Practical implications

Trying to obtain consensus on the impacts of pornography on forensic patients is not possible; material access decisions should be on a case-by-case basis. Policy decisions should be based on fully represented views, including the LGBTI community. The impacts on staff of their occupational exposure to such material should be recognised and support provided. Clinical decision-making in this area should consider not only if access should be allowed but also how it can be managed safely, if at all. This includes for all those who could be exposed to such material, inadvertently or otherwise.

Originality/value

It addresses the under-researched area of patient access to pornography, capturing a poorly considered sample, namely, high secure psychiatric.
A review of property rights in outer space from a land management perspective
Authors: de Vries Walter Timo , Hugentobler Urs
Published: July 2021

Purpose

In light of the discussions on outer space property management, this conceptual review paper aims to discuss and evaluate if, when and under which conditions certain land management and property right frameworks can apply to allocate and/or restrict property rights in outer space.

Design/methodology/approach

This paper applies a pragmatic review approach which seeks to better understand if and how the basic tenets of the land management frameworks could better shape and revise the challenges in outer space regulations.

Findings

Despite the fact that regulatory guidelines on outer space rights are existing, the analysis shows that these lack a number of practical tools and measures aiming at intervening if stakeholders do not follow the rules. With the use of land management frameworks, it is possible to derive policy options for making the outer space management more practical and action-oriented, in particular for the removal of space debris. These include amongst others more attention for formulating global public restrictions in outer space, incorporating regulatory guidelines for accessing open space regimes, addressing responsiveness and robustness in adherence and compliance to regulations

Research limitations/implications

Given the conceptual and discursive character of the paper, there are no specific empirical data, yet several recommendations for further research include expanding the boundary work between the land management and regulatory outer space domain.

Practical implications

The insights derived from land management and real estate related property theories could potentially provide new starting points for (re)formulating the regulatory framework for outer space property discourses.

Social implications

Interpreting the outer space regulations from known and practiced land management perspective helps to bridge the policy–society knowledge and necessity gap on outer space activities.

Originality/value

The specific land management perspective and discursive analysis on outer space debris provide new options for devising and extending regulatory guidelines for assigning responsibilities on outer space debris and debris rights, restrictions and responsibilities.
Customer experience research: intellectual structure and future research opportunities
Authors: Silva Jorge H.O. , Mendes Glauco H.S. , Cauchick Miguel Paulo A. , Amorim Marlene , Teixeira Jorge Grenha
Published: July 2021

Purpose

This article aims to synthesize and integrate current research on customer experience (CX), identifying the intellectual structure of the field, systematizing a conceptual framework and identifying future research opportunities.

Design/methodology/approach

To analyze 629 articles published in peer-reviewed journals in almost four decades, this study employs both bibliometric co-keyword and thematic literature analysis in a complementary way.

Findings

This article maps the CX literature by describing its intellectual structure in terms of three research domains (customer, organizational and technological), their corresponding most relevant research themes and topics. Moreover, this study develops a conceptual framework and research propositions to summarize and integrate the CX literature. This work recognizes technology as an important driver for the development of CX research. Lastly, this article provides future research opportunities for moving the field forward, considering an integrative view among domains.

Originality/value

This paper complements other reviews on CX by using a novel methodological approach (co-keyword and thematic analysis) that enables the identification and visualization of the CX intellectual structure. In addition, the study explores the increasing connection between technology and CX research, by raising evidence that technology, by continuously modifying services and consequently CX, has become a transversal component in the research field. These outcomes may be useful for academics and practitioners.
Managing material value creation matters in integrated reporting
Authors: Steenkamp Natasja , Roberts Roslyn
Published: July 2021

Purpose

This paper aims to explore how advanced integrated report preparers internalise and operationalise material value creation information to manage the generation of such information for the integrated report.

Design/methodology/approach

The paper adopts a qualitative approach using in-depth semi-structured interviews to examine how information about material value creation matters in six South African organisations are managed.

Findings

The findings will be useful to integrated reporting adopters as to how they might implement appropriate processes and systems to determine, communicate, collect and process information about matters that substantively affect their value creation.

Originality/value

The paper contributes to the body of knowledge by providing insight on how material value creation matters are determined, communicated internally and information about such matters generated.
Effect of silicone based softener on fabric handle properties made by ring, rotor and MVS yarns
Authors: Anam Wardah , Akhtar Khurram Shehzad , Ahmad Faheem , Rasheed Abher , Rasheed Abher , Mohsin Muhammad , Azam Farooq , Ullah Tehseen , Ahmad Sheraz
Published: July 2021

Purpose

The purpose of this study was to produce yarns from three different spinning techniques, i.e.Murata Vortex Spinning (MVS) ring spinning and rotor spinning. Those yarns were then used to produce fabrics. Then, the effect of silicone softener on tactile comfort of fabric was investigated.

Design/methodology/approach

Three different yarns, i.e. Ring, Rotor and MVS yarns, were used to make fabrics using CCI sample loom which were then subjected to post treatments like desizing, scouring and bleaching. After the completion of the dyeing process, silicone-based softener was used to improve the hand feel of fabrics. The structures of three yarns were evaluated using Scanning electron microscopy. The fabrics were evaluated against compression, bending and surface properties using Kawabata evaluation system.

Findings

The fabric made of MVS yarn depicted more geometrical roughness, coefficient of friction and bending rigidity but less compressibility as compared to fabrics made with other yarns. It was observed that softener concentration has a direct relationship with thickness and bending rigidity of the fabric, and inverse relationship with coefficient of friction and geometrical roughness of the fabric.

Originality/value

MVS yarn has some superior properties over rotor and ring spun yarn like high production rates, high resistance to pilling, clear appearance and stability against deformation but has disadvantage that it has less compressibility. Therefore, softener is applied on the fabric, to address this issue, so that it could also be used for apparels application.
Application of multi-objective optimization on the basis of ratio analysis (MOORA) for selection of cotton fabrics for optimal thermal comfort
Authors: Mitra Ashis
Published: July 2021

Purpose

Selection of fabrics for particular purposy-e has created lots of research interest over the years, and the said problems have been addressed by several researchers using various multi-criteria decision-making (MCDM) methods. The main purpose of this paper is to highlight a maiden approach to handle one such fabric selection problem using multi-objective optimization by ratio analysis (MOORA) as a simple yet potent MCDM tool.

Design/methodology/approach

Two approaches of MOORA method (namely, ratio system and reference point) have been demonstrated for ranking of 13 candidate fabrics based on four fabric attributes, namely, fabric cover, thickness, areal density and porosity.

Findings

In both the approaches, candidate fabric F3 secures rank 1 (the best alternative) whereas fabric F6 occupies rank 13 (the worst alternative). Moreover, ranking orders of these two approaches are alike and also show very high level of congruence with those of other approaches reported by earlier researchers, as evidenced from extremely high rank correlation coefficients (Rs > 0.89). During sensitivity analysis, each of the ranking results obtained from the four simulated weight sets do demonstrate very high degree of correlation (Rs > 0.90 for ratio system, andRs > 0.81 for reference point). Besides, no occurrence of rank reversal is observed even when the initial decision-making matrix is changed.

Originality/value

Most the methods adopted so far for fabric selection purpose involve huge mathematical equations, complex computation and/or logic. The uniqueness of the MOORA method is that it involves minimal and thus very simple mathematical operations although possesses higher level of robustness and reliability.
Fault detection and diagnosis using vibration signal analysis in frequency domain for electric motors considering different real fault types
Authors: Ribeiro Junior Ronny Francis , Areias Isac Antônio dos Santos , Gomes Guilherme Ferreira
Published: July 2021

Purpose

Electric motors are present in most industries today, being the main source of power. Thus, detection of faults is very important to rise reliability, reduce the production cost, improving uptime and safety. Vibration analysis for condition-based maintenance is a mature technique in view of these objectives.

Design/methodology/approach

This paper shows a methodology to analyze the vibration signal of electric rotating motors and diagnosis the health of the motor using time and frequency domain responses. The analysis lies in the fact that all rotating motor has a stable vibration pattern on health conditions. If the motor becomes faulty, the vibration pattern gets changed.

Findings

Results showed that through the vibration analysis using the frequency domain response it is possible to detect and classify the motors in several induced operation conditions: healthy, unbalanced, mechanical looseness, misalignment, bent shaft, broken bar and bearing fault condition.

Originality/value

The proposed methodology is verified through a real experimental setup.
Harmonious entrepreneurship – a new approach to the challenge of global sustainability
Authors: Kirby David Anthony , El-Kaffass Iman
Published: July 2021

Purpose

The article is intended to consider how entrepreneurship needs to adapt if it is to address the global sustainability challenge. The intention is to propose a new business model that recognises the interconnectedness of the global ecosystem.

Design/methodology/approach

The article analyses two case studies purposively written to demonstrate the difference between the traditional entrepreneurship approach, dating back to the 19th century and the proposed harmonised one. Both cases are based on secondary data and personal field observation.

Findings

While the two cases focus on wealth creation, job generation and innovation, the traditional approach is shown to have had a long-term deleterious impact on both society and the environment, whereas the proposed harmonised approach impacts positively. The article recognises the multifaceted nature of the sustainability challenge and that the three elements (economy/commerce, society and environment) are interconnected. If there is a change in the status of one the other, connected facets will change or will need to be changed. Thus any solution needs to address all three facets.

Social implications

The proposed business model will be of interest to scholars and practitioners of entrepreneurship and sustainability, as well as to policy makers and educators.

Originality/value

Apart from proposing a new business model that will address the sustainability challenge, the article provides a definition of harmonious entrepreneurship and identifies the conditions required for it to be met, as well as the characteristics of the harmonious entrepreneur.
A systematic review of video‐modelling interventions for children and adolescents with attention‐deficit hyperactivity disorder
Authors: Wilkes‐Gillan Sarah , Cordier Reinie , Chen Yu‐Wei , Swanton Ruth , Mahoney Natasha , Trimboli Concettina , Yule Elisa , Tam Elaine
Published: July 2021

Objective

To identify, appraise, and synthesise the evidence for video‐modelling interventions for individuals with attention‐deficit hyperactivity disorder (ADHD).

Method

We searched four electronic databases. Two independent researchers screened abstracts and methodologically assessed data using the Kmet appraisal checklist.

Results

A total of 15 studies met the inclusion criteria (11 original studies and four follow‐up studies). Of the 11 original studies, one was a randomised controlled trial, one was a controlled between‐group comparative design, two were one group pre‐test post‐test studies, one was an experimental 2 × 2 factorial design, and six were single‐case experimental design studies. Studies included 1–35 participants with ADHD aged 5–16 years. Three studies targeted behaviour, three targeted social play skills, two targeted social behaviour, one targeted social skills, one targeted goal orientation and friendship quality, targeted and one attention/comprehension of social behaviour. In four studies video‐modelling was the whole intervention, with no other intervention components reported. Nine studies reported positive outcomes immediately after intervention, two studies reported mixed findings. All studies were found to have good or strong methodological quality.

Conclusion

There is preliminary evidence to suggest video‐modelling may be a promising intervention approach for targeting the social skills and behaviours of individuals with ADHD when used in conjunction with other intervention components. Future studies need to lower the risk of bias and use larger sample sizes before the efficacy of video‐modelling interventions can be fully investigated.
Unilateral adrenalectomy for aldosterone‐producing adenoma in Hong Kong: Outcomes and factors predicting resolution of hypertension
Authors: Leung Hoi‐Tik , Yuen Wai‐Cheung , Lang Brian Hung‐Hin , Tan Kathryn Choon‐Beng , Fung Berry Tat‐Chow , Lau Emmy Yuen‐Fun , Ng Wai‐Kuen , Chan Kin‐Wah , Chan Bryant Shun‐Yan , Lau Ip‐Tim , Leung Jenny Yin‐Yan
Published: July 2021

Objective

To evaluate surgical outcomes of patients with aldosterone‐producing adenoma (APA) over a 6‐year period from 2009 to 2014 in Hong Kong, and to derive a new scoring system to predict cure of hypertension after surgery.

Methodology

This was a retrospective multi‐centre study evaluating 104 APA patients from five major hospitals in Hong Kong. These patients were retrieved using database from Surgical Outcomes Monitoring and Improvement Programme (SOMIP). Their clinical characteristics and surgical outcomes were evaluated.

Results

Over a median follow‐up period of 5 years, APA patients who had undergone adrenalectomy had an average decrease in systolic and diastolic blood pressure of 16.9 and 4.8 mm Hg respectively. Cure of hypokalaemia, cure of hypertension and improvement in hypertension occurred in 100%, 48.5% and 40% respectively. When a patient had two of the following three parameters, namely, female sex, duration of hypertension ≤5 years and number of anti‐hypertensives ≤2, the sensitivity and specificity in achieving cure in hypertension was 79.6% and 70.6%, respectively. Surgical complications were uncommon.

Conclusions

Adrenalectomy resulted in resolution of hypokalaemia for all and cure of hypertension in half of the patients with APA. It was safe and effective in curing hyperaldosteronism.
The impact of intensive care unit stay following oesophagectomy for oesophageal cancer on long‐term survival
Authors: Askari Alan , Wong Joshua , Rabinowitz Josh , Riaz Amjid
Published: July 2021

Aim

Survival in patients with oesophageal cancer is associated with an overall poor 5‐year survival rate. The aim of this study was to determine whether return to theatre, overall and intensive care unit (ICU) length of stay (LOS) impact long‐term survival.

Methods

A single‐institution dataset of prospectively collated data from January 2011 to December 2018 were analysed. Survival analysis using multivariable Cox regression analyses were undertaken to determine factors associated with survival.

Results

A total of 190 patients underwent an oesophagectomy, of whom 81.6% (155/190) were male and the median age was 66 years (interquartile range 58‐72 years). The median length of stay was 12 days (interquartile range 10‐15 days). At a mean follow‐up of 36 months, 41.9% (117/279) of the population were deceased. Nodal, N3 staging [hazard ratio (HR) 5.30, 95% confidence interval (CI) 2.92‐9.63] was associated with poorer survival. Prolonged ICU stay of 5 to 7 days (HR 2.26, 95% CI 1.18‐4.33, P = .015), 8 to 10 days (HR 3.96, 95% CI 1.65‐9.50, P = .002) and over 10 days (HR 2.47, 95% CI 1.11‐5.47, P = .027) was also associated with significantly worse survival.

Conclusion

Prolonged ICU stay after oesophagectomy has a negative impact on survival irrespective of the tumour stage. Enhanced recovery programs aimed at fast‐tracking ICU care and early safe patient discharge may improve long‐term survival.
Methodological threat or myth? Evaluating the current state of evidence on common method variance in human resource management research
Authors: Bozionelos Nikos , Simmering Marcia J.
Published: July 2021

New key evidence on common method variance (CMV) has been generated in the last decade (including quantitative and qualitative reviews, and simulations) to estimate its real validity threat, and evaluate the post hoc techniques to detect and correct for its effects. This work looks at the new evidence, and reviews all HRM‐related empirical articles published in the last 10 years in six major journals. The following primary conclusions are drawn. First, adoption of new knowledge about CMV by the empirical literature has been uneven. Second, published research in these journals indicates few incidences of meaningful distortion of estimates due to CMV, even when post hoc tests are used to detect it. Third, these findings in the empirical literature mirror the conclusions of reviews and simulations of the last 10 years, which indicate that the probability of significant distortion of estimates because of CMV is very limited.
R2Cross: A Web‐Based Decision Support Tool for Instream Flows
Authors: Oikonomou Panagiotis D. , Sidhu Rumpal , Wible Tyler , Morrison Ryan R.
Published: July 2021

The determination of instream flows for preserving or improving aquatic ecosystems’ health often includes challenges caused by varied hydraulic conditions, ecological criteria, and socioeconomic considerations. Despite the abundance of available methodologies, there are few available desktop or online applications enabling rapid assessment of instream flows that incorporate pertinent geospatial information for the region. We present R2Cross, a user‐friendly, online, instream flow rate calculation tool that was developed for aiding water allocation decisions in the state of Colorado. The R2Cross tool is the only existing online decision support tool based on a hydraulic rating method. It estimates hydraulic conditions at different water stages in a stream reach and compares them to habitat criteria to determine biological instream flow recommendations. Cloud‐based Geographic Information System features and additional tools are incorporated that augment information and aid in evaluating ecological needs. The development of the R2Cross tool enables an open‐access interface for management authorities and stakeholders to evaluate ecological needs at stream reaches. It automates the reporting process and could assist in making water management decisions about environmental protection. Although the R2Cross tool was developed for the state of Colorado, it is generalizable to other stream systems assuming similar habitat criteria.
A novel technique for a rare diagnosis: MRI‐guided vaginal biopsy of Skene gland adenocarcinoma: Pictorial Essay
Authors: Dentry Matt , McBean Rhiannon , Garrett Andrea , Brown Nicholas
Published: July 2021

FDG‐PET and MRI imaging of the pelvis identified a suspicious lesion in a patient with a history of small cell neuroendocrine tumour of the vagina, and after a negative surgical biopsy, a multidisciplinary team meeting decided to proceed with MRGB (magnetic resonance‐guided biopsy). The patient remains well and in remission two years after anterior exenteration, with final histology of the lesion determined to be Skene gland adenocarcinoma. MRGB is a novel and effective way to diagnose vaginal lesions.
Durable response to EGFR tyrosine kinase inhibitors in a patient with non–small cell lung cancer harboring an EGFR kinase domain duplication
Authors: Hirokawa Esuteru , Watanabe Satomi , Sakai Kazuko , Takeda Masayuki , Sato Chihiro , Takahama Takayuki , Nishio Kazuto , Nakagawa Kazuhiko
Published: July 2021

Epidermal growth factor receptor (EGFR) kinase domain duplication (KDD) has been identified as an oncogenic driver in 0.05% to 0.14% of non–small cell lung cancer (NSCLC) patients. However, little is known of the efficacy of EGFR tyrosine kinase inhibitors (TKIs) for such patients. Here, we report the case of a 45‐year‐old Japanese woman with NSCLC positive for EGFR‐KDD (duplication of exons 18–25) who developed carcinomatous meningitis and showed a marked response to the EGFR‐TKIs erlotinib and osimertinib. As far as we are aware, this is the first report of EGFR‐TKI efficacy for carcinomatous meningitis in a NSCLC patient harboring EGFR‐KDD.
Encouraging participation in colorectal cancer screening for people with schizophrenia: A randomized controlled trial
Authors: Fujiwara Masaki , Yamada Yuto , Shimazu Taichi , Kodama Masafumi , So Ryuhei , Matsushita Takanori , Yoshimura Yusaku , Horii Shigeo , Fujimori Maiko , Takahashi Hirokazu , Nakaya Naoki , Kakeda Kyoko , Miyaji Tempei , Hinotsu Shiro , Harada Keita , Okada Hiroyuki , Uchitomi Yosuke , Yamada Norihito , Inagaki Masatoshi
Published: July 2021

Objective

We examined the efficacy of a case management approach to improve participation in colorectal cancer screening among people with schizophrenia.

Methods

This was a randomized, parallel group trial. We recruited outpatients with schizophrenia aged 40 years or over from two psychiatric hospitals in Japan. Participants were randomly assigned (1:1) to treatment as usual or case management intervention plus treatment as usual using a web‐based system. Attending clinicians and participants were unmasked to the allocation. Case management included education and patient navigation for colorectal cancer screening using a fecal occult blood test. Treatment as usual included direct mail government recommendations. The primary endpoint was participation in colorectal cancer screening assessed using municipal records. We also assessed the secondary endpoint of participation in other cancer screenings (lung, gastric, breast, and cervical).

Results

Between 3 June and 9 September 2019, 172 eligible participants were randomly assigned to the case management plus treatment as usual group (n = 86) or treatment as usual group (n = 86). One participant was ineligible and another withdrew consent; both were excluded from analysis. A significantly higher proportion of participants received colorectal cancer screening in the case management plus treatment as usual group than in the treatment as usual group (40 [47.1%] of 85 participants vs. 10 [11.8%] of 85 participants, p < 0.0001). The proportion of lung cancer screening also increased. No serious adverse events associated with the study intervention occurred.

Conclusion

The case management intervention to encourage participation in colorectal cancer screening was effective for patients with schizophrenia.
Molecular definition of severe acute respiratory syndrome coronavirus 2 receptor‐binding domain mutations: Receptor affinity versus neutralization of receptor interaction
Authors: Vogel Monique , Augusto Gilles , Chang Xinyue , Liu Xuelan , Speiser Daniel , Mohsen Mona O. , Bachmann Martin F.
Published: July 2021

Background

Several new variants of SARS‐CoV‐2 have emerged since fall 2020 which have multiple mutations in the receptor‐binding domain (RBD) of the spike protein. It is unclear which mutations affect receptor affinity versus immune recognition.

Methods

We produced wild type RBD, RBD with single mutations (E484K, K417N, or N501Y) or with all three mutations combined and tested their binding to ACE2 by biolayer interferometry (BLI). The ability of convalescent sera to recognize RBDs and block their interaction with ACE2 was tested as well.

Results

We demonstrated that single mutation N501Y increased binding affinity to ACE2 but did not strongly affect its recognition by convalescent sera. In contrast, single mutation E484K had almost no impact on the binding kinetics, but essentially abolished recognition of RBD by convalescent sera. Interestingly, combining mutations E484K, K417N, and N501Y resulted in a RBD with both features: enhanced receptor binding and abolished immune recognition.

Conclusions

Our data demonstrate that single mutations either affect receptor affinity or immune recognition while triple mutant RBDs combine both features.
Standardisation of allergen products: 4. Validation of a candidate European Pharmacopoeia standard method for quantification of major grass pollen allergen Phl p 5
Authors: Zimmer Julia , Schmidt Sandra , Kaul Susanne , Costanzo Angèle , Buchheit Karl‐Heinz , Brown Shannon , Carnés Jerónimo , Chapman Martin , Chen Aaron , De Neergaard Michalade , Döring Sascha , Hindley James Phillip , Holzhauser Thomas , Jorajuria Sylvie , Le Tallec David , Lombardero Manuel , Iacovacci Patrizia , Reese Gerald , Sander Ingrid , Smith Bryan , Strecker Daniel , van Ree Ronald , Zebina Myriam , Vieths Stefan
Published: July 2021

Background

The aim of the BSP090 project is the establishment of European Pharmacopoeia Chemical Reference Substances (CRSs) in combination with corresponding standard ELISA methods for quantification of major allergens in allergen products. Here, we present data of a Phl p 5‐specific sandwich ELISA that proved suitable for the quantification of Phl p 5, one of the major Timothy grass (Phleum pratense) pollen allergens.

Methods

A Phl p 5‐specific ELISA system was assessed with respect to accuracy, precision, inter‐assay (within laboratory) and inter‐laboratory variations, in a ring trial including 14 laboratories in Europe and the USA. Model samples containing recombinant Phl p 5a CRS as well as native grass pollen extracts were analysed. Each participant was instructed to perform at least one preliminary assay to familiarise with the protocol, followed by three independent assays.

Results

The candidate standard ELISA proved suitable to quantify recombinant and native Phl p 5 with satisfactory precision (93% of results within ±30% acceptance range). Inter‐assay variation (max. GCV 24%) and especially inter‐laboratory variation (max. GCV 13%) showed conclusive results. When assessing accuracy by means of recovery of recombinant spikes from a grass pollen extract matrix, similarly satisfactory spike recovery results were observed for the two spikes with higher concentrations (all within ±30% acceptance range), whereas recovery of the lowest concentration spike was slightly poorer with mean results of six laboratories exceeding acceptance range.

Conclusions

Based on the collaborative study results, the assessed Phl p 5‐specific immunoassay is appropriate to be proposed as European Pharmacopoeia standard method.
Researchers succeed in limited ex‐utero mouse embryogenesis
Authors: Han Jason J.
Published: July 2021

Certain aspects of early intrauterine development of mammals have been a mystery to scientists for centuries. This study has successfully recreated parts of embryogenesis outside of the uterus in mice.