bluuebunny/crossref_metadata_2025 huggingface dataset:
An Automatic Navigation System for Unmanned Surface Vehicles in Realistic Sea Environments
Authors: Sun Xiaojie , Wang Guofeng , Fan Yunsheng , Mu Dongdong , Qiu Bingbing
Published: January 2018

In recent years, unmanned surface vehicles (USVs) have received notable attention because of their many advantages in civilian and military applications. To improve the autonomy of USVs, this paper describes a complete automatic navigation system (ANS) with a path planning subsystem (PPS) and collision avoidance subsystem (CAS). The PPS based on the dynamic domain tunable fast marching square (DTFMS) method is able to build an environment model from a real electronic chart, where both static and dynamic obstacles are well represented. By adjusting the S a t u r a t i o n , the generated path can be changed according to the requirements for security and path length. Then it is used as a guidance trajectory for the CAS through a dynamic target point. In the CAS, according to finite control set model predictive control (FCS-MPC) theory, a collision avoidance control algorithm is developed to track trajectory and avoid collision based on a three-degree of freedom (DOF) planar motion model of USV. Its target point and security evaluation come from the planned path and environmental model of the PPS. Moreover, the prediction trajectory of the CAS can guide changes in the dynamic domain model of the vessel itself. Finally, the system has been tested and validated using the situations of three types of encounters in a realistic sea environment.
Mathematical Modelling of Nitrate Removal from Water Using a Submerged Membrane Adsorption Hybrid System with Four Adsorbents
Authors: Kalaruban Mahatheva , Loganathan Paripurnanda , Shim Wang , Kandasamy Jaya , Vigneswaran Saravanamuthu
Published: January 2018

Excessive concentrations of nitrate in ground water are known to cause human health hazards. A submerged membrane adsorption hybrid system that includes a microfilter membrane and four different adsorbents (Dowex 21K XLT ion exchange resin (Dowex), Fe-coated Dowex, amine-grafted (AG) corn cob and AG coconut copra) operated at four different fluxes was used to continuously remove nitrate. The experimental data obtained in this study was simulated mathematically with a homogeneous surface diffusion model that incorporated membrane packing density and membrane correlation coefficient, and applied the concept of continuous flow stirred tank reactor. The model fit with experimental data was good. The surface diffusion coefficient was constant for all adsorbents and for all fluxes. The mass transfer coefficient increased with flux for all adsorbents and generally increased with the adsorption capacity of the adsorbents.
Effect of HCV Core Antigen and RNA Clearance during Therapy with Direct Acting Antivirals on Hepatic Stiffness Measured with Shear Wave Elastography in Patients with Chronic Viral Hepatitis C
Authors: Łucejko Mariusz , Flisiak Robert
Published: January 2018

To assess a combination of novel measures of therapeutic success in the treatment of chronic hepatitis C (CHC) infection, we evaluated liver stiffness (LS) with shear wave elastography and hepatitis C virus core antigen (HCVcAg) concentrations. We followed 34 patients during and after treatment with direct acting antivirals. All patients achieved a sustained virologic and serologic response and a significant increase of albumin levels. Decreases of alanine aminotransferase (ALT) activity and alpha-fetoprotein (AFP) level were observed during the treatment and follow-up period. A significant decrease in LS was observed between baseline, end of treatment (EOT), and at 24- and 96-week post-treatment follow-up. LS decline between EOT and 96-week follow-up (FU96) was observed in 79% of patients. Significant LS changes were seen in patients with advanced fibrosis, particularly in cirrhotics and in patients with ALT exceeding 100 IU/mL. There was a positive correlation between ALT activity and LS changes at the baseline versus FU96. A negative correlation was demonstrated between individual HCVcAg baseline concentrations and reduction of LS at the baseline versus FU96. In conclusion, we observed that LS significantly declined during and after antiviral treatment. It was accompanied by improvement in some liver function measures, and disappearance of both HCVcAg and HCV ribonucleic acid (HCV RNA).
Changes in Summer Pressure Patterns across the Late 1960s and Their Influence on Temperature Trends on the Eastern Coast of the Iberian Peninsula
Authors: Favà Vicent , Curto Juan , Llasat Maria
Published: January 2018

During the second half of the 20th century, in the littoral and pre-littoral areas of the Valencia region, the diurnal temperature range (DTR) registered a significant drop in summer (July and August). Meanwhile, in the same period in the lower Ebro Valley (Ebro Observatory), to the north of the Valencia region, the maximum temperature and DTR increased steeply. In order to explain the DTR drop in the coastal areas of the Valencia region, some studies have proposed an increase in the summer sea-surface temperature (SST) and others have attributed it to the urban heat island effect. Nevertheless, this drop occurred well before the current climate change was evident and therefore, before the rise of the SST. Furthermore, regarding the second proposed explanation, the drop in the DTR does not disappear when working with selected stations away from heavily urbanized areas. In this work, we propose that both the DTR rise at the Ebro Observatory and the DTR drop in the Valencia region are linked to the same process: changes in atmospheric circulation on a synoptic-scale in the North Atlantic that occurred in the late 1960s.
Building Materials, Ionizing Radiation and HBIM: A Case Study from Pompei (Italy)
Authors: Argenziano Pasquale , Avella Alessandra , Albanese Stefano
Published: January 2018

This paper presents a different point of view on the conservation of the built heritage, adding ionizing radiation to the most well-known digital documentation dataset. Igneous building materials characterize most of the built heritage in the Campania region, and in a large part of southern Italy. The ionizing radiations proceeding from these materials can produce stochastic biological effects on the exposed living beings. The research team designed and tested a technical-scientific protocol to survey and analyse this natural phenomenon in association with the use of geological material for building purposes. Geographical Information Systems (GISs), City Information Modelling (CIM), and Building Information Modelling (BIM) are the digital tools used to manage the construction entities and their characteristics, and then to represent the thematic data as false-colour images. The emission spectra of fair-faced or plastered materials as a fingerprint of their nature is proposed as a non-invasive method. Due to both the huge presence of historical buildings and an intense touristic flow, the main square of Pompei has been selected as a study area.
Vine Shoots and Grape Stalks as Carbon Sources for Hydrogen Evolution Reaction Electrocatalyst Supports
Authors: Cardoso J.A.S.B. , Šljukić B. , Erdem M. , Sequeira C.A.C. , Santos D.M.F.
Published: January 2018

Activated bio-based carbons produced from vine shoots (VSAC) and grape stalks (GSAC), which have larger surface area and total pore volume than most of the commercially available activated carbons, are used as supports for palladium nanoparticles (Pd NPs). The prepared materials are characterised by elemental analysis, N2-sorption, X-ray diffraction, scanning electron microscopy with energy dispersive X-ray spectroscopy, and transmission electron microscopy analysis and are then assessed as potential cathodes for the hydrogen evolution reaction (HER) in alkaline media. The electrocatalysts’ performance for HER is evaluated from cathodic polarisation curves at different temperatures and compared to that of Vulcan XC72-supported Pd NPs. Additional chronoamperometry studies helped to assess the electrocatalysts’ activity stability. The novel VSAC-supported Pd electrocatalyst exhibits good HER activity in terms of high current density at low overpotentials, leading to the best performance.
Effects of Contingency versus Constraints on the Body-Mass Scaling of Metabolic Rate
Authors: Glazier Douglas
Published: January 2018

I illustrate the effects of both contingency and constraints on the body-mass scaling of metabolic rate by analyzing the significantly different influences of ambient temperature (Ta) on metabolic scaling in ectothermic versus endothermic animals. Interspecific comparisons show that increasing Ta results in decreasing metabolic scaling slopes in ectotherms, but increasing slopes in endotherms, a pattern uniquely predicted by the metabolic-level boundaries hypothesis, as amended to include effects of the scaling of thermal conductance in endotherms outside their thermoneutral zone. No other published theoretical model explicitly predicts this striking variation in metabolic scaling, which I explain in terms of contingent effects of Ta and thermoregulatory strategy in the context of physical and geometric constraints related to the scaling of surface area, volume, and heat flow across surfaces. My analysis shows that theoretical models focused on an ideal 3/4-power law, as explained by a single universally applicable mechanism, are clearly inadequate for explaining the diversity and environmental sensitivity of metabolic scaling. An important challenge is to develop a theory of metabolic scaling that recognizes the contingent effects of multiple mechanisms that are modulated by several extrinsic and intrinsic factors within specified constraints.
Strategies for Development and Improvement of the Urban Fabric: A Vienna Case Study
Authors: Vuckovic Milena , Maleki Aida , Mahdavi Ardeshir
Published: January 2018

Numerous studies have shown that densely developed and populated urban areas experience significant anthropogenic heat flux and elevated concentrations of air pollutants and CO2, with consequences for human health, thermal comfort, and well-being. This may also affect the atmospheric composition and circulation patterns within the urban boundary layer, with consequences for local, regional, and global climate. One of the resulting local implications is the increase in urban air temperature. In this context, the present contribution explores urban fabric development and mitigation strategies for two locations in the city of Vienna, Austria. Toward this end, the potential of specific planning and mitigation strategies regarding urban overheating was assessed using a state-of-the-art CFD-based (computational fluid dynamics) numeric simulation environment. The results display different levels of effectiveness for selected design and mitigation measures under a wide range of boundary conditions.
Effect of Sorbitol Templates on the Preferential Crystallographic Growth of Isotactic Polypropylene Wax
Authors: Quiñones-Jurado Zoe , Ávila-Orta Carlos , Castillo-Reyes Blanca , Mata-Padilla José , Hsiao Benjamin , Medellín-Rodríguez Francisco , Waldo-Mendoza Miguel
Published: January 2018

The crystallization of isotactic polypropylene wax (iPP) in the presence of different sorbitol structures was studied. Dibenzylidene Sorbitol (DBS), as well as two of its derivatives with one or two methyl groups in the DBS molecule (MDBS and DMDBS, respectively), were tested as nanometer-size fibrillar templates. The early nucleation stage and crystal morphology were analyzed in Real-Time Wide-Angle X-ray Scattering (WAXS) and polarized optical microscopy (POM). It was found that the iPP crystals showed an α-phase unit cell for the three different sorbitols. However, a preferential crystal growth in the plane (040) was observed for iPP–MDBS. The macrostructure morphology of the iPP–DBS and iPP–DMDBS wax compounds was spherulitic, while nodular macrocrystals were observed for the iPP–MDBS compound. It was concluded that the MDBS template promoted a lower interface energy because of its match with the c-axis of the iPP wax crystals, whereas, in the case of the DBS and DMDBS templates, the preferential plane was the (110), characteristic of the iPP spherulitic arrangement.
Formation of Quasicrystalline Phases and Their Close Approximants in Cast Al-Mn Base Alloys Modified by Transition Metals
Authors: Stan-Głowińska Katarzyna
Published: January 2018

The aim of the presented research was to study the influence of Cr, Co, Ni, and Cu additions on the formation of quasicrystalline particles in the 94Al-6Mn base alloy during casting at intermediate cooling rates. Based on the obtained results, Cu and Ni enhance quasicrystalline phase nucleation compared to the unmodified binary composition. In the case of Cu addition, formation of a quasicrystalline phase takes place along whole thickness of the prepared casting, but its fraction and morphology depends on the cooling rates present in different parts of the sample. Based on the previous works on the beneficial effect of Fe addition, a quaternary alloy containing both Fe and Cu was prepared to evaluate the effect of the simultaneous presence of these elements on the microstructure of the obtained castings.
The Shannon Entropy Trend of a Fish System Estimated by a Machine Vision Approach Seems to Reflect the Molar Se:Hg Ratio of Its Feed
Authors: Eguiraun Harkaitz , Casquero Oskar , Martinez Iciar
Published: January 2018

The present study investigates the suitability of a machine vision-based method to detect deviations in the Shannon entropy (SE) of a European seabass (Dicentrarchus labrax) biological system fed with different selenium:mercury (Se:Hg) molar ratios. Four groups of fish were fed during 14 days with commercial feed (control) and with the same feed spiked with 0.5, 5 and 10 mg of MeHg per kg, giving Se:Hg molar ratios of 29.5 (control-C1); 6.6, 0.8 and 0.4 (C2, C3 and C4). The basal SE of C1 and C2 (Se:Hg > 1) tended to increase during the experimental period, while that of C3 and C4 (Se:Hg < 1) tended to decrease. In addition, the differences in the SE of the four systems in response to a stochastic event minus that of the respective basal states were less pronounced in the systems fed with Se:Hg molar ratios lower than one (C3 and C4). These results indicate that the SE may be a suitable indicator for the prediction of seafood safety and fish health (i.e., the Se:Hg molar ratio and not the Hg concentration alone) prior to the displaying of pathological symptoms. We hope that this work can serve as a first step for further investigations to confirm and validate the present results prior to their potential implementation in practical settings.
Physiological Effects of Visual Stimulation with Forest Imagery
Authors: Song Chorong , Ikei Harumi , Miyazaki Yoshifumi
Published: January 2018

This study was aimed to clarify the physiological effects of visual stimulation using forest imagery on activity of the brain and autonomic nervous system. Seventeen female university students (mean age, 21.1 ± 1.0 years) participated in the study. As an indicator of brain activity, oxyhemoglobin (oxy-Hb) concentrations were measured in the left and right prefrontal cortex using near-infrared time-resolved spectroscopy. Heart rate variability (HRV) was used as an indicator of autonomic nervous activity. The high-frequency (HF) component of HRV, which reflected parasympathetic nervous activity, and the ratio of low-frequency (LF) and high-frequency components (LF/HF), which reflected sympathetic nervous activity, were measured. Forest and city (control) images were used as visual stimuli using a large plasma display window. After sitting at rest viewing a gray background for 60 s, participants viewed two images for 90 s. During rest and visual stimulation, HRV and oxy-Hb concentration in the prefrontal cortex were continuously measured. Immediately thereafter, subjective evaluation of feelings was performed using a modified semantic differential (SD) method. The results showed that visual stimulation with forest imagery induced (1) a significant decrease in oxy-Hb concentrations in the right prefrontal cortex and (2) a significant increase in perceptions of feeling “comfortable,” “relaxed,” and “natural.”
Sex-Specific Associations in Nutrition and Activity-Related Risk Factors for Chronic Disease: Australian Evidence from Childhood to Emerging Adulthood
Authors: Hoare Erin , Dash Sarah , Jennings Garry , Kingwell Bronwyn
Published: January 2018

Global assessments of burden of disease suggests there are sex differences in risk factors for chronic disease, including overweight/obesity, dietary patterns and habitual physical activity. Given that prevention efforts aim to target such factors to reduce disease risk, the age at which sex differences may occur is of particular interest. Early life to young adulthood is the optimal time for intervention, with lifestyle habits typically forming during this period. This study aimed to identify the sex differences in risk factors for chronic disease during childhood (5–9 years), adolescence (10–17 years) and emerging adulthood (18–25 years) in a large population-representative Australian sample. Among children in this study (n = 739), no sex-related differences were observed. Among adolescents (n = 1304), females were more likely than males to meet daily fruit and vegetable recommendations (12.9% vs. 7.5%; OR = 1.84, 95% CI = 1.16, 2.93, p < 0.05). Among emerging adults (n = 909), females were less likely to be overweight/obese (30.1% vs. 39.8%; OR = 0.65, 95% CI = 0.44, 0.95, p < 0.05) and more likely to meet physical activity recommendations (52.1% vs. 42.3%; OR = 1.44, 95% CI = 1.01, 2.06, p < 0.05). These findings suggest that sex differences for risk factors of chronic disease occur during adolescence and emerging adulthood, although the differences are not consistent across age periods. From adolescence onwards, it appears that females exhibit lower risk factors than males and a life span approach to risk factor monitoring is warranted.
Characterization and Oral Delivery of Proinsulin-Transferrin Fusion Protein Expressed Using ExpressTec
Authors: Chen Yu-Sheng , Zaro Jennica , Zhang Deshui , Huang Ning , Simon Andrew , Shen Wei-Chiang
Published: January 2018

Proinsulin-transferrin fusion protein (ProINS-Tf) has been designed and successfully expressed from the mammalian HEK293 cells (HEK-ProINS-Tf). It was found that HEK-ProINS-Tf could be converted into an activated form in the liver. Furthermore, HEK-ProINS-Tf was demonstrated as an extra-long acting insulin analogue with liver-specific insulin action in streptozotocin (STZ)-induced type 1 diabetic mice. However, due to the low production yield from transfected HEK293 cells, there are other interesting features, including the oral bioavailability, which have not been fully explored and characterized. To improve the protein production yield, an alternative protein expression system, ExpressTec using transgenic rice (Oryza sativa L.), was used. The intact and active rice-derived ProINS-Tf (ExpressTec-ProINS-Tf) was successfully expressed from the transgenic rice expression system. Our results suggested that, although the insulin-like bioactivity of ExpressTec-ProINS-Tf was slightly lower in vitro, its potency of in vivo blood glucose control was considerably stronger than that of HEK-ProINS-Tf. The oral delivery studies in type 1 diabetic mice demonstrated a prolonged control of blood glucose to near-normal levels after oral administration of ExpressTec-ProINS-Tf. Results in this report suggest that ExpressTec-ProINS-Tf is a promising insulin analog with advantages including low cost, prolonged and liver targeting effects, and most importantly, oral bioactivity.
Galectin-3: One Molecule for an Alphabet of Diseases, from A to Z
Authors: Sciacchitano Salvatore , Lavra Luca , Morgante Alessandra , Ulivieri Alessandra , Magi Fiorenza , De Francesco Gian , Bellotti Carlo , Salehi Leila , Ricci Alberto
Published: January 2018

Galectin-3 (Gal-3) regulates basic cellular functions such as cell–cell and cell–matrix interactions, growth, proliferation, differentiation, and inflammation. It is not surprising, therefore, that this protein is involved in the pathogenesis of many relevant human diseases, including cancer, fibrosis, chronic inflammation and scarring affecting many different tissues. The papers published in the literature have progressively increased in number during the last decades, testifying the great interest given to this protein by numerous researchers involved in many different clinical contexts. Considering the crucial role exerted by Gal-3 in many different clinical conditions, Gal-3 is emerging as a new diagnostic, prognostic biomarker and as a new promising therapeutic target. The current review aims to extensively examine the studies published so far on the role of Gal-3 in all the clinical conditions and diseases, listed in alphabetical order, where it was analyzed.
Corylin Suppresses Hepatocellular Carcinoma Progression via the Inhibition of Epithelial-Mesenchymal Transition, Mediated by Long Noncoding RNA GAS5
Authors: Chen Chi-Yuan , Chen Chin-Chuan , Shieh Tzong-Ming , Hsueh Chuen , Wang Shu-Huei , Leu Yann-Lii , Lian Jang-Hau , Wang Tong-Hong
Published: January 2018

Corylin is a flavonoid extracted from the nuts of Psoralea corylifolia L. (Fabaceae), which is a widely used anti-inflammatory and anticancer herb in China. Recent studies revealed antioxidant, anti-inflammatory, and bone differentiation–promoting effects of corylin. However, there are no studies examining the anticancer activity of corylin. In this study, we used cells and animal models to examine the antitumor effects of corylin on hepatocellular carcinoma (HCC) and then studied its downstream regulatory mechanisms. The results showed that corylin significantly inhibited the proliferation, migration, and invasiveness of HCC cells and suppressed epithelial–mesenchymal transition. We found that the anti-HCC mechanism of corylin’s action lies in the upregulation of tumor suppressor long noncoding RNA growth arrest-specific transcript 5 (GAS5) and the activation of its downstream anticancer pathways. In animal experiments, we also found that corylin can significantly inhibit tumor growth without significant physiological toxicity. The above results suggest that corylin has anti-HCC effects and good potential as a clinical treatment.
Structural Studies of Predicted Ligand Binding Sites and Molecular Docking Analysis of Slc2a4 as a Therapeutic Target for the Treatment of Cancer
Authors: Aruleba Raphael , Adekiya Tayo , Oyinloye Babatunji , Kappo Abidemi
Published: January 2018

Presently, many studies have focused on exploring in silico approaches in the identification and development of alternative therapy for the treatment and management of cancer. Solute carrier family-2-member-4-gene (Slc2a4) which encodes glucose transporter 4 protein (GLUT4), has been identified as a promising therapeutic target for cancer. Though Slc2a4 is known to play a major regulatory role in the pathophysiology of type 2 diabetes, emerging evidence suggests that successful pharmacological inhibition of this protein may lead to the development of a novel drug candidate for the treatment of cancer. In this study, Slc2a4 protein sequence was retrieved and analysed using in silico approaches, and we identified seven putative antimicrobial peptides (AMPs; RAB1-RAB7) as anti-cancer. The structures of the protein and AMPs were modelled using I-TASSER server, and the overall quality of the Slc2a4 model was validated using PROCHECK. Subsequently, the probable motifs and active site of the protein were forecasted. Also, the molecular interaction between the AMPs and Slc2a4 was ascertained using PatchDock. The result revealed that, all the AMPs are good Slc2a4 inhibitors with RAB1 having the highest binding affinity of 12,392 and binding energy of −39.13 kcal/mol. Hence, this study reveals that all the generated AMPs can serve as therapeutic drug in treating cancer by inhibiting Slc2a4 which is responsible for the production of energy for cancer cells during angiogenesis. This is the first report on AMPs as inhibitors of Slc2a4 for the treatment of cancer.
Multifunctional Tannic Acid/Silver Nanoparticle-Based Mucoadhesive Hydrogel for Improved Local Treatment of HSV Infection: In Vitro and In Vivo Studies
Authors: Szymańska Emilia , Orłowski Piotr , Winnicka Katarzyna , Tomaszewska Emilia , Bąska Piotr , Celichowski Grzegorz , Grobelny Jarosław , Basa Anna , Krzyżowska Małgorzata
Published: January 2018

Mucoadhesive gelling systems with tannic acid modified silver nanoparticles were developed for effective treatment of herpes virus infections. To increase nanoparticle residence time after local application, semi solid formulations designed from generally regarded as safe (GRAS) excipients were investigated for their rheological and mechanical properties followed with ex vivo mucoadhesive behavior to the porcine vaginal mucosa. Particular effort was made to evaluate the activity of nanoparticle-based hydrogels toward herpes simplex virus (HSV) type 1 and 2 infection in vitro in immortal human keratinocyte cell line and in vivo using murine model of HSV-2 genital infection. The effect of infectivity was determined by real time quantitative polymerase chain reaction, plaque assay, inactivation, attachment, penetration and cell-to-cell assessments. All analyzed nanoparticle-based hydrogels exhibited pseudoplastic and thixotropic properties. Viscosity and mechanical measurements of hydrogels were found to correlate with the mucoadhesive properties. The results confirmed the ability of nanoparticle-based hydrogels to affect viral attachment, impede penetration and cell-to-cell transmission, although profound differences in the activity evoked by tested preparations toward HSV-1 and HSV-2 were noted. In addition, these findings demonstrated the in vivo potential of tannic acid modified silver nanoparticle-based hydrogels for vaginal treatment of HSV-2 genital infection.
Comparison of Human Dermal Fibroblasts and HaCat Cells Cultured in Medium with or without Serum via a Generic Tissue Engineering Research Platform
Authors: Gabbott Christopher , Sun Tao
Published: January 2018

A generic research platform with 2-dimensional (2D) cell culture technology, a 3-dimensional (3D) in vitro tissue model, and a scaled-down cell culture and imaging system in between, was utilized to address the problematic issues associated with the use of serum in skin tissue engineering. Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium were compared and analyzed via the platform. It was demonstrated that serum depletion had significant influence on the attachment of HaCat cells onto tissue culture plastic (TCP), porous substrates and cellulosic scaffolds, which was further enhanced by the pre-seeded HDFs. The complex structures formed by the HDFs colonized within the porous substrates and scaffolds not only prevented the seeded HaCat cells from filtering through the open pores, but also acted as cellular substrates for HaCat cells to attach onto. When mono-cultured on TCP, both HDFs and HaCat cells were less proliferative in medium without serum than with serum. However, both cell types were successfully co-cultured in 2D using serum-free medium if the initial cell seeding density was higher than 80,000 cells/cm2 (with 1:1 ratio). Based on the results from 2D cultures, co-culture of both cell types on modular substrates with small open pores (125 μm) and cellulosic scaffolds with open pores of varying sizes (50–300 µm) were then conducted successfully in serum-free medium. This study demonstrated that the generic research platform had great potential for in-depth understanding of HDFs and HaCat cells cultivated in serum-free medium, which could inform the processes for manufacturing skin cells or tissues for clinical applications.
Aspects of Newborn Screening in Isovaleric Acidemia
Authors: Schlune Andrea , Riederer Anselma , Mayatepek Ertan , Ensenauer Regina
Published: January 2018

Isovaleric acidemia (IVA), an inborn error of leucine catabolism, is caused by mutations in the isovaleryl-CoA dehydrogenase (IVD) gene, resulting in the accumulation of derivatives of isovaleryl-CoA including isovaleryl (C5)-carnitine, the marker metabolite used for newborn screening (NBS). The inclusion of IVA in NBS programs in many countries has broadened knowledge of the variability of the condition, whereas prior to NBS, two distinct clinical phenotypes were known, an “acute neonatal” and a “chronic intermittent” form. An additional biochemically mild and potentially asymptomatic form of IVA and its association with a common missense mutation, c.932C>T (p.A282V), was discovered in subjects identified through NBS. Deficiency of short/branched chain specific acyl-CoA dehydrogenase (2-methylbutyryl-CoA dehydrogenase), a defect of isoleucine degradation whose clinical significance remains unclear, also results in elevated C5-carnitine, and may therefore be detected by NBS for IVA. Treatment strategies for the long-term management of symptomatic IVA comprise the prevention of catabolism, dietary restriction of natural protein or leucine intake, and supplementation with l-carnitine and/or l-glycine. Recommendations on how to counsel and manage individuals with the mild phenotype detected by NBS are required.
Pedagogy before Technology: A Design-Based Research Approach to Enhancing Skills Development in Paramedic Science Using Mixed Reality
Authors: Cowling Michael , Birt James
Published: January 2018

In health sciences education, there is growing evidence that simulation improves learners’ safety, competence, and skills, especially when compared to traditional didactic methods or no simulation training. However, this approach to simulation becomes difficult when students are studying at a distance, leading to the need to develop simulations that suit this pedagogical problem and the logistics of this intervention method. This paper describes the use of a design-based research (DBR) methodology, combined with a new model for putting ‘pedagogy before technology’ when approaching these types of education problems, to develop a mixed reality education solution. This combined model is used to analyse a classroom learning problem in paramedic health sciences with respect to student evidence, assisting the educational designer to identify a solution, and subsequently develop a technology-based mixed reality simulation via a mobile phone application and three-dimensional (3D) printed tools to provide an analogue approximation for an on-campus simulation experience. The developed intervention was tested with students and refined through a repeat of the process, showing that a DBR process, supported by a model that puts ‘pedagogy before technology’, can produce over several iterations a much-improved simulation that results in a simulation that satisfies student pedagogical needs.
Intraneural Platelet-Rich Plasma Injections for the Treatment of Radial Nerve Section: A Case Report
Authors: García de Cortázar Unai , Padilla Sabino , Lobato Enrique , Delgado Diego , Sánchez Mikel
Published: January 2018

The radial nerve is the most frequently injured nerve in the upper extremity. Numerous options in treatment have been described for radial nerve injury, such as neurolysis, nerve grafts, or tendon transfers. Currently, new treatment options are arising, such as platelet-rich plasma (PRP), an autologous product with proved therapeutic effect for various musculoskeletal disorders. We hypothesized that this treatment is a promising alternative for this type of nerve pathology. The patient was a healthy 27-year-old man who suffered a deep and long cut in the distal anterolateral region of the right arm. Forty-eight hours after injury, an end-to-end suture was performed without a microscope. Three months after the surgery, an electromyogram (EMG) showed right radial nerve neurotmesis with no tendency to reinnervation. Four months after the trauma, serial intraneural infiltrations of PRP were conducted using ultrasound guidance. The therapeutic effect was assessed by manual muscle testing and by EMG. Fourteen months after the injury and 11 months after the first PRP injection, functional recovery was achieved. The EMG showed a complete reinnervation of the musculature of the radial nerve dependent. The patient remains satisfied with the result and he is able to practice his profession. Conclusions: PRP infiltrations have the potential to enhance the healing process of radial nerve palsy. This case report demonstrates the therapeutic potential of this technology for traumatic peripheral nerve palsy, as well as the apt utility of US-guided PRP injections.
Fungi as Endophytes in Artemisia thuscula: Juxtaposed Elements of Diversity and Phylogeny
Authors: Cosoveanu Andreea , Rodriguez Sabina Samuel , Cabrera Raimundo
Published: January 2018

Artemisia is a plant genus highly studied for its medicinal applications. The studies on the associated fungal endophytes are scarce. Ten plants specimens of Artemisia thuscula from Tenerife and La Palma were sampled to isolate the endophytic fungi. Identification of the endophytic fungi was based on morphology, Internal Transcribed Spacer (ITS) and Large Subunit (LSU) regions sequencing and indicates 37 fungal species affiliated to 25 fungal genera. Colonization rate varied among plants (CR = 25% to 92.11%). The most dominant colonizers found were Alternaria alternata (CF = 18.71%), Neofusicoccum sp. (CF = 8.39%) and Preussia sp. (CF = 3.23). Tendency for host specificity of most endophytic fungal species was observed. Sorensen–Dice index revealed that of 45 cases in the matrix, 27 of them were of zero similarity. Further, only one case was found to have 57% similarity (TF2 and TF7) and one case with 50% similarity (TF1 and TF4). The rest of the cases had values ranging between 11% and 40% similarity. Diversity indices like Brillouin, Margalef species richness, Simpson index of diversity and Fisher’s alpha, revealed plants from La Palma with higher values than plants from Tenerife. Three nutrient media (i.e., potato dextrose agar―PDA, lignocellulose agar―LCA, and tomato juice agar―V8) were used in a case study and revealed no differences in terms of colonization rate when data was averaged. Colonization frequency showed several species with preference for nutrient medium (63% of the species were isolated from only one nutrient medium). For the phylogenetic reconstruction using the Bayesian method, 54 endophytic fungal ITS sequences and associated GenBank sequences were analyzed. Ten orders (Diaporthales, Dothideales, Botryosphaeriales, Hypocreales, Trichosphaeriales, Amphisphaeriales, Xylariales, Capnodiales, Pleosporales and Eurotiales) were recognized. Several arrangements of genera draw the attention, like Aureobasidium (Dothideales) and Aplosporella (Botryosphaeriales) which are clustered with a recent ancestor (BS = 0.97).
Genetic Variations in Sweet Taste Receptor Gene Are Related to Chocolate Powder and Dietary Fiber Intake in Obese Children and Adolescents
Authors: Pioltine Marina , De Melo Maria , Santos Aritânia , Machado Alisson , Fernandes Ariana , Fujiwara Clarissa , Cercato Cintia , Mancini Marcio
Published: January 2018

Childhood obesity is a major public health problem. It has a direct impact on the quality of life of children and adolescents, as well as on their future risk of developing chronic diseases. Dietary patterns rich in fats and sugars and lacking dietary fibers, vitamins, and minerals, as well as lack of physical exercise have been associated with the rise of obesity prevalence. However, factors that contribute to the preference for foods rich in these nutrients are not well established. Taste is recognized as an important predictor of food choices, and polymorphisms in taste-related genes may explain the variability of taste preference and food intake. The aim of this research is to evaluate the influence of polymorphisms of the sweet taste receptor gene TAS1R2 on diet and metabolic profile in obese children and adolescents. A cross-sectional study with 513 obese children and adolescents and 135 normal-weight children was carried out. A molecular study was performed for the single nucleotide polymorphisms (SNPs) rs9701796 and rs35874116 of TAS1R2, and dietary intake, anthropometric parameters (weight, height, waist circumference, waist-to-height ratio (WHtR)), and metabolic profile (including fasting glucose, insulin, triglyceride, high-density lipoprotein (HDL)–cholesterol, and leptin levels) were analyzed. The variant rs9701796 was associated with increased waist-height ratio, as well as with a higher chocolate powder intake in obese children. The variant rs35874116 was associated with a lower dietary fiber intake. In conclusion, there was no relationship between genotypes and risk of obesity. Obese adolescents carrying the serine allele of SNP rs9701796 in TAS1R2 showed higher waist-to-height ratio and chocolate powder intake, whereas those carrying the valine allele of SNP rs35874116 in TAS1R2 were characterized by lower dietary fiber intake.
Microstructures and Properties of Laser Cladding Al-TiC-CeO2 Composite Coatings
Authors: He Xing , Kong Dejun , Song Renguo
Published: January 2018

Al-TiC-CeO2 composite coatings have been prepared by using a laser cladding technique, and the microstructure and properties of the resulting composite coatings have been investigated using scanning electron microscopy (SEM), a 3D microscope system, X-ray diffraction (XRD), micro-hardness testing, X-ray stress measurements, friction and wear testing, and an electrochemical workstation. The results showed that an Al-Fe phase appears in the coatings under different applied laser powers and shows good metallurgical bonding with the matrix. The dilution rate of the coating first decreases and then increases with increasing laser power. The coating was transformed from massive and short rod-like structures into a fine granular structure, and the effect of fine grain strengthening is significant. The microhardness of the coatings first decreases and then increases with increasing laser power, and the maximum microhardness can reach 964.3 HV0.2. In addition, the residual stress of the coating surface was tensile stress, and crack size increases with increasing stress. When the laser power was 1.6 kW, the coating showed high corrosion resistance.
X-ray Absorption Fine Structure (XAFS) Studies of Oxide Glasses—A 45-Year Overview
Authors: Mastelaro Valmor , Zanotto Edgar
Published: January 2018

X-ray Absorption Fine Structure (XAFS) spectroscopy has been widely used to characterize the short-range order of glassy materials since the theoretical basis was established 45 years ago. Soon after the technique became accessible, mainly due to the existence of Synchrotron laboratories, a wide range of glassy materials was characterized. Silicate glasses have been the most studied because they are easy to prepare, they have commercial value and are similar to natural glasses, but borate, germanate, phosphate, tellurite and other less frequent oxide glasses have also been studied. In this manuscript, we review reported advances in the structural characterization of oxide-based glasses using this technique. A focus is on structural characterization of transition metal ions, especially Ti, Fe, and Ni, and their role in different properties of synthetic oxide-based glasses, as well as their important function in the formation of natural glasses and magmas, and in nucleation and crystallization. We also give some examples of XAFS applications for structural characterization of glasses submitted to high pressure, glasses used to store radioactive waste and medieval glasses. This updated, comprehensive review will likely serve as a useful guide to clarify the details of the short-range structure of oxide glasses.
Preparation and Characterization of MoB Coating on Mo Substrate
Authors: Wang Yi , Shi Haiyan , Yan Jianhui , Wang Dezhi
Published: January 2018

A molybdenum boride (MoB) coating was prepared on molybdenum (Mo) by an in-situ chemical vapor deposition method. To develop a fuller understanding of the effect of depositional conditions on the growth of MoB coating formed on Mo substrate, this paper studied the phase composition and microstructure of the coating at varied conditions. At the same time, the relationships between the growth of the coating in thickness and depositional conditions were also investigated. The phase composition of the coating, which was prepared using 0.2 wt % boron (B), was composed of MoB and Mo2B phases. The single MoB phase was obtained using a high B content (above 0.2 wt %). The microstructure of the coating showed that MoB coating could be prepared at temperatures over 900 °C. With the increase of temperature and time, the thickness of the coating increased. The growth of the coating was also influenced by the content of NaF. With increasing content, the thickness of the coating first increased, and then decreased. In addition, the prepared coating had a high hardness (3130.85 HV), and the mass change of the coated sample was only 4.92 mg/cm2 after isothermal oxidation at 600 °C for 100 h. The MoB coating could provide a good oxidation resistance for Mo at 600 °C.
Process Chain for the Production of a Bimetal Component from Mg with a Complete Al Cladding
Authors: Förster Wolfgang , Binotsch Carolin , Awiszus Birgit
Published: January 2018

With respect to its density, magnesium (Mg) has a high potential for lightweight components. Nevertheless, the industrial application of Mg is limited due to, for example, its sensitivity to corrosion. To increase the applicability of Mg, a process chain for the production of a Mg component with a complete aluminum (Al) cladding is presented. Hydrostatic co-extrusion was used to produce bar-shaped rods with a diameter of 20 mm. The bonding between the materials was verified by ultrasonic testing. Specimens with a length of 79 mm were cut off from the rods and forged by using a two-staged process. After the first step (Heading), the Mg core was removed partially by drilling to ensure a complete enclosing of the remaining Mg during the second forging step (Net shape forging). The geometry of the drilling hole and the heading die design were dimensioned with the Finite Element-simulation software FORGE. Hence, a complete Al-enclosed Mg component was achieved by using the described process chain and forming processes. Microstructural investigations confirm the formation of an intermetallic interface as expected.
Design and Performance Assessment of a Solid-State Microcooler for Thermal Neuromodulation
Authors: Fernandes José , Vendramini Estelle , Miranda Ana , Silva Cristiana , Dinis Hugo , Coizet Veronique , David Olivier , Mendes Paulo
Published: January 2018

It is well known that neural activity can be modulated using a cooling device. The applications of this technique range from the treatment of medication-resistant cerebral diseases to brain functional mapping. Despite the potential benefits of such technique, its use has been limited due to the lack of suitable thermal modulators. This paper presents the design and validation of a solid-state cooler that was able to modulate the neural activity of rodents without the use of large and unpractical water pipes. A miniaturized thermal control solution based exclusively on solid-state devices was designed, occupying only 5 mm × 5 mm × 3 mm, and featuring the potential for wireless power and communications. The cold side of the device was cooled to 26 °C, while the hot side was kept below 43 °C. This range of temperatures is compatible with brain cooling and efficient enough for achieving some control of neural activity.
Analysis of PCR Kinetics inside a Microfluidic DNA Amplification System
Authors: Chen Jyh Jian , Li Kun Tze
Published: January 2018

In order to analyze the DNA amplification numerically with integration of the DNA kinetics, three-dimensional simulations, including flow and thermal fields, and one-dimensional polymerase chain reaction (PCR) kinetics are presented. The simulated results are compared with experimental data that have been applied to the operation of a continuous-flow PCR device. Microchannels fabricated by Micro Electro-Mechanical Systems (MEMS) technologies are shown. Comprehensive simulations of the flow and thermal fields and experiments measuring temperatures during thermal cycling are presented first. The resultant velocity and temperature profiles from the simulations are introduced to the mathematical models of PCR kinetics. Then kinetic equations are utilized to determine the evolution of the species concentrations inside the DNA mixture along the microchannel. The exponential growth of the double-stranded DNA concentration is investigated numerically with the various operational parameters during PCR. Next a 190-bp segment of Bartonella DNA is amplified to evaluate the PCR performance. The trends of the experimental results and numerical data regarding the DNA amplification are similar. The unique architecture built in this study can be applied to a low-cost portable PCR system in the future.
Manipulating Microrobots Using Balanced Magnetic and Buoyancy Forces
Authors: Feng Lin , Wu Xiaocong , Jiang Yonggang , Zhang Deyuan , Arai Fumihito
Published: January 2018

We present a novel method for the three-dimensional (3D) control of microrobots within a microfluidic chip. The microrobot body contains a hollow space, producing buoyancy that allows it to float in a microfluidic environment. The robot moves in the z direction by balancing magnetic and buoyancy forces. In coordination with the motion of stages in the xy plane, we achieved 3D microrobot control. A microgripper designed to grasp micron-scale objects was attached to the front of the robot, allowing it to hold and deliver micro-objects in three dimensions. The microrobot had four degrees of freedom and generated micronewton-order forces. We demonstrate the microrobot’s utility in an experiment in which it grips a 200 μm particle and delivers it in a 3D space.
Responses of an Agricultural Soil Microbiome to Flooding with Seawater after Managed Coastal Realignment
Authors: Sjøgaard Kamilla , Valdemarsen Thomas , Treusch Alexander
Published: January 2018

Coastal areas have become more prone to flooding with seawater due to climate-change-induced sea-level rise and intensified storm surges. One way to cope with this issue is by “managed coastal realignment”, where low-lying coastal areas are no longer protected and instead flooded with seawater. How flooding with seawater impacts soil microbiomes and the biogeochemical cycling of elements is poorly understood. To address this, we conducted a microcosm experiment using soil cores collected at the nature restoration project site Gyldensteen Strand (Denmark), which were flooded with seawater and monitored over six months. Throughout the experiment, biogeochemical analyses, microbial community fingerprinting and the quantification of marker genes documented clear shifts in microbiome composition and activity. The flooding with seawater initially resulted in accelerated heterotrophic activity that entailed high ammonium production and net removal of nitrogen from the system, also demonstrated by a concurrent increase in the abundances of marker genes for ammonium oxidation and denitrification. Due to the depletion of labile soil organic matter, microbial activity decreased after approximately four months. The event of flooding caused the largest shifts in microbiome composition with the availability of labile organic matter subsequently being the most important driver for the succession in microbiome composition in soils flooded with seawater.