bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimized Ship-Radiated Noise Feature Extraction Approaches Based on CEEMDAN and Slope Entropy
Authors: Li Yuxing , Tang Bingzhao , Jiao Shangbin
Published: September 2022

Slope entropy (Slopen) has been demonstrated to be an excellent approach to extracting ship-radiated noise signals (S-NSs) features by analyzing the complexity of the signals; however, its recognition ability is limited because it extracts the features of undecomposed S-NSs. To solve this problem, in this study, we combined complete ensemble empirical mode decomposition with adaptive noise (CEEMDAN) to explore the differences of Slopen between the intrinsic mode components (IMFs) of the S-NSs and proposed a single-IMF optimized feature extraction approach. Aiming to further enhance its performance, the optimized combination of dual-IMFs was selected, and a dual-IMF optimized feature extraction approach was also proposed. We conducted three experiments to demonstrate the effectiveness of CEEMDAN, Slopen, and the proposed approaches. The experimental and comparative results revealed both of the proposed single- and dual-IMF optimized feature extraction approaches based on Slopen and CEEMDAN to be more effective than the original ship signal-based and IMF-based feature extraction approaches.
Do Regional Heads Utilize Capital Expenditures, Grants, and Social Assistance in the Context of Elections?
Authors: Rizqiyati Chanif , Setiawan Doddy
Published: September 2022

This study empirically analyzes the alleged existence of political budget cycles in Indonesian local governments. Using panel data, this quantitative study concentrates on capital expenditures, grant expenditures, and social assistance expenditures in the election year and the year before the election. The purposive sampling method is used to take the sample—a local government that experienced two election cycles from 2012 to 2018. The sample consists of 1306 observational data points on capital expenditures and grants, and 636 observational data points on social assistance. The study tests its hypothesis using multiple regression analysis. The results indicate that the election year negatively affects capital expenditures, positively affects grants, and has no effect on social assistance. The year before the election negatively affects capital expenditures, but positively affects grants and social assistance. The incumbent positively affects social assistance but does not affect capital expenditures and grants. Transfers have a negative effect on capital expenditures, a positive effect on grants, and no effect on social assistance. The interactions of the election year and year before the election with the incumbent is not proven in this study.
Influence of Glycerol on Methanol Fuel Characteristics and Engine Combustion Performance
Authors: Jin Chao , Sun Tianyun , Xu Teng , Jiang Xueli , Wang Min , Zhang Zhao , Wu Yangyi , Zhang Xiaoteng , Liu Haifeng
Published: September 2022

Methanol derived from solar energy is a carbon-neutral alternative fuel for engines. The low viscosity of methanol is one of the problems that restrict its direct compression ignition application in engines. Glycerol is a renewable resource derived from biomass, and its viscosity is more than 1700 times that of methanol. In this study, glycerol was mixed with methanol in different volume fractions (1–50%), and a methanol-glycerol mixture with similar viscosity to diesel was prepared. Then, the particle size, electrical conductivity, viscosity, swelling and corrosion characteristics of the mixed fuel were measured. Finally, the combustion and emission tests of methanol-glycerol mixed fuel were carried out on a heavy-duty multi-cylinder diesel engine. The results show that glycerol can effectively adjust the viscosity of the mixed fuel. The viscosity of the mixed fuel can reach 3.19 mm2/s at 20 °C when blended with 30% glycerol by volume, which meets the requirements of the national standard for diesel fuel. The addition of glycerol can alleviate the corrosion of methanol to the polymer. The test of the mixed fuel in the direct compression ignition engine shows that the thermal efficiency of methanol mixed with 5% glycerol was further improved than that of pure methanol, both of which were significantly higher than the thermal efficiency of diesel compression ignition engines. Methanol and 5% glycerol by volume blends can reduce soot and nitrogen oxide emissions while maintaining low HC and CO emissions. Therefore, proper blending of glycerol in methanol fuel can optimize the fuel properties of methanol and achieve higher thermal efficiency and lower pollutant emissions than pure methanol direct compression ignition.
Reservoir Characteristics and Controlling Factors of Sedimentary Pyroclastic Rocks in Deep-Buried Basins: A Case Study of Yingtai Fault Depression, Southern Songliao Basin
Authors: Zhao Ranlei , Xu Xiao , Ma Wentao , Li Cunlei , Zhang Qiushi , Yue Qingyou
Published: September 2022

In this article, based on core description, thin section, scanning electron microscope (SEM), well logging and reservoir physical properties, the reservoir controlling factors of sedimentary pyroclastic rocks in deep-buried basins are assessed via the relation between reservoirs and defining factors, including lithological characteristics, sedimentary microfacies and diagenesis. In addition, the contributing factors of anomalously high-porosity and high-permeability zone are analyzed. The lithological characteristics and diagenesis of the sedimentary pyroclastic rocks are closely related to reservoirs. The reservoir porosity–permeability of sedimentary pyroclastic rocks with large volcanic clastic particles is better than in those with small volcanic clastic particles. Sedimentary pyroclastic rocks with high content of unstable clastic particles, such as feldspar and rock debris, are easier to form the high-quality reservoirs than those with high content of quartz. The dissolution is the most important and direct reason to form the anomalously high-porosity and high-permeability zones of the sedimentary pyroclastic rocks in deep-buried basins. It is concluded that the size and composition of the clastic particles in the sedimentary pyroclastic rocks are the internal-controlling factors of the effective reservoirs, while the diagenetic fluid and the burial process are the external-controlling factors which form the effective reservoirs.
Medicinal Treatment of Elderly Psoriasis Patients before and after Entering a Nursing Home
Authors: Petersen Jana , Garbe Claudia , Wolf Sandra , Stephan Brigitte , Augustin Matthias , Hagenström Kristina
Published: September 2022

Psoriasis (PS) is a chronic inflammatory skin disease, and it increasingly appears also in the elderly population. There is a rising interest in drug therapy for PS, especially for people receiving care in nursing homes (NH). Which PS-related drugs are prescribed in the time before nursing home admission (NHA), and to what extent does the supply of drugs change after NHA? Which specialties prescribe PS-related drugs? Statutory health insurance data were examined for people with PS, aged ≥ 65 years, who were newly admitted to a NH in the period 2011–2014 and observed for one year before and after NHA. Changes in prescription prevalence (pre-post comparison) were examined for significant differences. Prescriptions of PS-relevant drugs were measured by defined daily dose and stratified according to the prescribing specialist group. The analysis included 718 insured persons with PS (76.2% female, mean age 83.3 years). Systemic therapeutics played a minor role (pre: 2.6% vs. post: 2.1%) in drug therapy. Topical steroids had a high share of about 40% in the pre–post comparison. Overall, the proportion of people with PS who received treatment remained at a comparable level before and after NHA. A structured assessment of the skin is crucial, specifically in people with cognitive impairment.
Human Evidence of Perfluorooctanoic Acid (PFOA) Exposure on Hepatic Disease: A Systematic Review and Meta-Analysis
Authors: Choi Jihee , Kim Jong-Yeon , Lee Hae-Jeung
Published: September 2022

Background: Perfluorooctanoic acid (PFOA) is widely used throughout different industries, including the food industry, because it is resistant to heat and prevents water or oil from easily permeating into or contaminating materials coated by PFOA. Although many studies have reported an association between PFOA exposure and the risk of developing hepatic diseases, it is still in debate because they have shown conflicting results. Therefore, this study conducted a systematic review and meta-analysis on the relationship between PFOA exposure and hepatic diseases. Methods: This study searched studies related to hepatic diseases due to PFOA exposure until 31 December 2021, using PubMed, EMBASE, and Web of Science. This study performed a systematic review and meta-analysis through research question development, literature screening, data extraction, and risk of bias evaluation. This study found 8280 studies after excluding duplicate literature and selected 5 studies in the final stage. Among them, two studies were included in the meta-analysis. Results: The results of the meta-analysis showed that the ALT of people exposed to PFOA was 117% higher than the ALT of those not exposed to PFOA, and it was significantly different (OR = 1.167; 95% CI, 1.086–1.254). Conclusion: However, since the number of studies included in the analysis was not large enough to conclude that PFOA exposure was associated with the development of hepatic diseases, more observational studies are needed to confirm its long-term effects.
A Kirkpatrick Model Process Evaluation of Reactions and Learning from My Strengths Training for Life™
Authors: Quinton Mary L. , Tidmarsh Grace , Parry Benjamin J. , Cumming Jennifer
Published: September 2022

Underpinned by the New World Kirkpatrick model, and in the context of a community-based sport psychology programme (My Strengths Training for Life™) for young people experiencing homelessness, this process evaluation investigated (1) young peoples’ reactions (i.e., program and facilitator evaluation, enjoyment, attendance, and engagement) and learning (i.e., mental skills and transfer intention), (2) the relationship between reaction and learning variables, and (3) the mediators underpinning this relationship. A total of 301 young people living in a West Midlands housing service completed questionnaires on demographics and reaction and learning variables. Higher levels of programme engagement were positively associated with more favourable reactions to the programme. Enjoyment positively predicted learning outcomes, which was mediated by transfer intention. Recommendations are made for (1) a balance between rigor and flexibility for evaluation methods with disadvantaged youth, (2) including engagement as well as attendance as indicators of meaningful programme participation, (3) measuring programme experiences (e.g., enjoyment) to understand programme effectiveness, and (4) providing opportunities for skill transfer during and after programme participation. Our findings have implications for researchers, programme commissioners, and policymakers designing and evaluating programmes in community-based settings.
Side Bioimpedance Analysis in Menopausal Post-Oncological Breast Cancer
Authors: Bifolco Giuseppe , Pinazzi Antonio , Bini Vittorio , Stefani Laura
Published: September 2022

Background: Post-oncological BC (breast cancer) has an increased cardiovascular risk due to the variation of some anthropometric parameters. This study investigates the differences between a quadrantectomy and a mastectomy on the body composition over time in presence of a breast prothesis. Methods: A group of BC patients (n = 41 aged 56.6 ± 9.5 years; 15 mastectomy patients; and 26 quadrantectomy patients) were compared to controls (C) (n = 22 aged 46.5 ± 13.44 years). Through bioimpedance analysis (Akern-BIA 101), the body mass index (BMI), total body water (TBW), extracellular water (ECW), body cell mass (BCM), fat mass (FM), free fat mass (FFM), and angle phase (PA) were compared within each group and between different groups using the Student’s Test T. Results: The BC group showed lower values of FFM and TBW compared to C. The FFM was significantly (p = 0.04) lower in those with quadrantectomy. The right hemisome of the quadrantectomy has increased values of FFM, BCM (p = 0.04) and TBW compared to the counter-lateral hemisome, and FM values (p = 0.0008) lower than the counter-lateral. The hemisome with intervention has increased values of FM and ECW compared to the counter lateral, as well the FFM, BCM, TBW, and PA. Conclusions: The results support the hypothesis that non-conservative surgical treatment (mastectomy) is associated with a better BIA profile without any substantial impact of breast implants in the body composition analysis. The awareness of a severe diseases could play a role to ameliorate lifestyle; however, further studies will be necessary to support this theory.
Effects of a Personalised Physical Exercise Program on University Workers Overall Well-Being: “UAL-Activa” Program
Authors: Casimiro-Andújar Antonio Jesús , Martín-Moya Ricardo , Maravé-Vivas María , Ruiz-Montero Pedro Jesús
Published: September 2022

Background and objectives: Regular individualised physical exercise (PE) is a habit that not only has consequences for overall health (physical, emotional, social and mental) but can also have positive effects on organisations and institutions, as it helps workers to improve their personal balance and recover from the effort of their working day, showing higher levels of energy, commitment and productivity. The aim of this study was to understand the relationship between the practice of PE and well-being in personal life and at work, as well as job satisfaction, overall health and the assessment of the personal training service provided by final-year students studying for a degree in Physical Activity and Sport Sciences. Methods: This study used a qualitative research methodology. There were 25 employees of the University of Almeria (UAL) (M = 52.16 ± 9.55 years), divided into two focus groups and participating in the physical exercise program “UAL Activa”. Results: The following three main themes, based on the results, emerged: (a) social well-being during PE practice, (b) assessment of the personal training service and (c) physical exercise as an emotional benefit at work. Conclusion. The workers’ interventions have shown that participating in a personalised PE program led by a personal trainer can improve overall health and mood, with a very positive influence on the working environment.
Residential Exposure to Dampness Is Related to Reduced Level of Asthma Control among Adults
Authors: Jaakkola Maritta S. , Hyrkäs-Palmu Henna , Jaakkola Jouni J. K.
Published: September 2022

We assessed potential relations between indicators of indoor dampness and mold exposures at home and the level of asthma control among adults. The present population-based cross-sectional study, the Northern Finnish Asthma Study (NoFAS), included 1995 adult subjects with bronchial asthma who replied to study questionnaires (response rate: 40.4%). The Asthma Control Test (ACT) was used as the measure of asthma control. We calculated the mean difference in ACT score (ACTdifference) and the risk ratio (RR) of poor asthma control (ACT ≤ 19) for the exposure and reference groups and applied Poisson regression to adjust for potential confounding. Exposure to indoor dampness at home was related to a significantly reduced level of asthma control (ACTdifference: −0.83, 95% CI: −1.60 to −0.07), especially among men (ACTdifference: −2.68, 95% CI: −4.00 to −1.37). Water damage (aRR = 1.29, 95% CI: 1.01, 1.65) and indoor dampness, especially among men (aRR = 1.92, 95% CI: 1.15, 3.20), increased the risk of poor asthma control. We provide evidence that exposure to indoor dampness at home reduces asthma control among adults, especially in men. Indoor visible mold and mold odor were not significantly related to asthma control. Advice on how to prevent indoor dampness at home should be an important part of asthma management.
Identifying Drug Targets of Oral Squamous Cell Carcinoma through a Systems Biology Method and Genome-Wide Microarray Data for Drug Discovery by Deep Learning and Drug Design Specifications
Authors: Lin Yi-Chung , Chen Bor-Sen
Published: September 2022

In this study, we provide a systems biology method to investigate the carcinogenic mechanism of oral squamous cell carcinoma (OSCC) in order to identify some important biomarkers as drug targets. Further, a systematic drug discovery method with a deep neural network (DNN)-based drug–target interaction (DTI) model and drug design specifications is proposed to design a potential multiple-molecule drug for the medical treatment of OSCC before clinical trials. First, we use big database mining to construct the candidate genome-wide genetic and epigenetic network (GWGEN) including a protein–protein interaction network (PPIN) and a gene regulatory network (GRN) for OSCC and non-OSCC. In the next step, real GWGENs are identified for OSCC and non-OSCC by system identification and system order detection methods based on the OSCC and non-OSCC microarray data, respectively. Then, the principal network projection (PNP) method was used to extract core GWGENs of OSCC and non-OSCC from real GWGENs of OSCC and non-OSCC, respectively. Afterward, core signaling pathways were constructed through the annotation of KEGG pathways, and then the carcinogenic mechanism of OSCC was investigated by comparing the core signal pathways and their downstream abnormal cellular functions of OSCC and non-OSCC. Consequently, HES1, TCF, NF-κB and SP1 are identified as significant biomarkers of OSCC. In order to discover multiple molecular drugs for these significant biomarkers (drug targets) of the carcinogenic mechanism of OSCC, we trained a DNN-based drug–target interaction (DTI) model by DTI databases to predict candidate drugs for these significant biomarkers. Finally, drug design specifications such as adequate drug regulation ability, low toxicity and high sensitivity are employed to filter out the appropriate molecular drugs metformin, gefitinib and gallic-acid to combine as a potential multiple-molecule drug for the therapeutic treatment of OSCC.
Mast Cells Are Activated in the Giant Earlobe Keloids: A Case Series
Authors: Nakajima Yukari , Aramaki Noriko , Takeuchi Nao , Yamanishi Ayumi , Kumagai Yoshiko , Okabe Keisuke , Yokoyama Tomoaki , Kishi Kazuo
Published: September 2022

Mast cells and inflammatory cells are abundant in keloid and hypertrophic scar tissues. Even if the cause of physical injury is similar, such as piercing or scratching with hands, clinical findings show differences in the size of keloids in the same area. Hence, we performed histological studies on giant keloids larger than the earlobe, and other smaller keloids. We also examined the risk factors associated with the formation of giant lesions. No statistically significant differences in the association of the risk factors were observed. However, histological observations clearly showed a high number of degranulated or active mast cells with a trend towards a greater number of degranulated mast cells in the giant keloid tissues. Collagen production also tended to increase. Two patients with giant keloids were severely obese, suggesting that the persistent inflammatory state of obesity may also be involved in the growth of keloid lesions.
Allyl Isothiocyanate (AITC) Induces Apoptotic Cell Death In Vitro and Exhibits Anti-Tumor Activity in a Human Glioblastoma GBM8401/luc2 Model
Authors: Lu Kung-Wen , Lu Tai-Jung , Chueh Fu-Shin , Lai Kuang-Chi , Hsia Te-Chun , Peng Shu-Fen , Cheng Ching-Chang , Chou Yu-Cheng , Hsu Fei-Ting
Published: September 2022

Some clinically used anti-cancer drugs are obtained from natural products. Allyl isothiocyanate (AITC), a plant-derived compound abundant in cruciferous vegetables, has been shown to possess an anti-cancer ability in human cancer cell lines in vitro, including human brain glioma cells. However, the anti-cancer effects of AITC in human glioblastoma (GBM) cells in vivo have not yet been examined. In the present study, we used GBM8401/luc2 human glioblastoma cells and a GBM8401/luc2-cell-bearing animal model to identify the treatment efficacy of AITC. Here, we confirm that AITC reduced total cell viability and induced cell apoptosis in GBM8401/luc2 cells in vitro. Furthermore, Western blotting also showed that AITC induced apoptotic cell death through decreased the anti-apoptotic protein BCL-2, MCL-1 expression, increased the pro-apoptotic protein BAX expression, and promoted the activities of caspase-3, -8, and -9. Therefore, we further investigated the anti-tumor effects of AITC on human GBM8401/luc2 cell xenograft mice. The human glioblastoma GBM8401/luc2 cancer cells were subcutaneously injected into the right flank of BALB/c nude mice to generate glioblastoma xenograft mice. The animals were randomly divided into three groups: group I was treated without AITC (control); group II with 0.1 mg/day of AITC; and group III with 0.2 mg/day of AITC every 3 days for 27 days. Bodyweight, and tumor volume (size) were recorded every 3 days. Tumors exhibiting Luc2 intensity were measured, and we quantified intensity using Living Image software on days 0, 12, and 24. After treatment, tumor weight from each mouse was recorded. Tumor tissues were examined for histopathological changes using H&E staining, and we analyzed the protein levels via immunohistochemical analysis. Our results indicate that AITC significantly inhibited tumor growth at both doses of AITC due to the reduction in tumor size and weight. H&E histopathology analysis of heart, liver, spleen, and kidney samples revealed that AITC did not significantly induce toxicity. Body weight did not show significant changes in any experiment group. AITC significantly downregulated the protein expression levels of MCL-1, XIAP, MMP-9, and VEGF; however, it increased apoptosis-associated proteins, such as cleaved caspase-3, -8, and -9, in the tumor tissues compared with the control group. Based on these observations, AITC exhibits potent anti-cancer activity in the human glioblastoma cell xenograft model via inhibiting tumor cell proliferation and the induction of cell apoptosis. AITC may be a potential anti-GBM cancer drug that could be used in the future.
The Role of IL-7 and IL-7R in Cancer Pathophysiology and Immunotherapy
Authors: Wang Chunli , Kong Lingzu , Kim Seokmin , Lee Sunyoung , Oh Sechan , Jo Seona , Jang Inhwan , Kim Tae-Don
Published: September 2022

Interleukin-7 (IL-7) is a multipotent cytokine that maintains the homeostasis of the immune system. IL-7 plays a vital role in T-cell development, proliferation, and differentiation, as well as in B cell maturation through the activation of the IL-7 receptor (IL-7R). IL-7 is closely associated with tumor development and has been used in cancer clinical research and therapy. In this review, we first summarize the roles of IL-7 and IL-7Rα and their downstream signaling pathways in immunity and cancer. Furthermore, we summarize and discuss the recent advances in the use of IL-7 and IL-7Rα as cancer immunotherapy tools and highlight their potential for therapeutic applications. This review will help in the development of cancer immunotherapy regimens based on IL-7 and IL-7Rα, and will also advance their exploitation as more effective and safe immunotherapy tools.
Elevated Serum Cortisol Levels in Patients with Focal Epilepsy, Depression, and Comorbid Epilepsy and Depression
Authors: Druzhkova Tatyana A. , Yakovlev Alexander A. , Rider Flora K. , Zinchuk Mikhail S. , Guekht Alla B. , Gulyaeva Natalia V.
Published: September 2022

Background: The hypothalamic-pituitary-adrenal (HPA) axis, inflammatory processes and neurotrophic factor systems are involved in pathogenesis of both epilepsy and depressive disorders. The study aimed to explore these systems in patients with focal epilepsy (PWE, n = 76), epilepsy and comorbid depression (PWCED n = 48), and major depressive disorder (PWMDD, n = 62) compared with healthy controls (HC, n = 78). Methods: Parameters of the HPA axis, neurotrophic factors, and TNF-α were measured in blood serum along with the hemogram. Results: Serum cortisol level was augmented in PWE, PWCED, and PWMDD compared with HC and was higher in PWMDD than in PWE. Serum cortisol negatively correlated with Mini–Mental State Examination (MMSE) score in PWE, and positively with depression inventory–II (BDI-II) score in PWMDD. Only PWMDD demonstrated elevated plasma ACTH. Serum TNF-α, lymphocytes, and eosinophils were augmented in PWMDD; monocytes elevated in PWE and PWCED, while neutrophils were reduced in PWE and PWMDD. Serum BDNF was decreased in PWE and PWCED, CNTF was elevated in all groups of patients. In PWE, none of above indices depended on epilepsy etiology. Conclusions: The results confirm the involvement of HPA axis and inflammatory processes in pathogenesis of epilepsy and depression and provide new insights in mechanisms of epilepsy and depression comorbidity.
Single-Cell RNA Sequencing Reveals Molecular Features of Heterogeneity in the Murine Retinal Pigment Epithelium
Authors: Pandey Ravi S. , Krebs Mark P. , Bolisetty Mohan T. , Charette Jeremy R. , Naggert Jürgen K. , Robson Paul , Nishina Patsy M. , Carter Gregory W.
Published: September 2022

Transcriptomic analysis of the mammalian retinal pigment epithelium (RPE) aims to identify cellular networks that influence ocular development, maintenance, function, and disease. However, available evidence points to RPE cell heterogeneity within native tissue, which adds complexity to global transcriptomic analysis. Here, to assess cell heterogeneity, we performed single-cell RNA sequencing of RPE cells from two young adult male C57BL/6J mice. Following quality control to ensure robust transcript identification limited to cell singlets, we detected 13,858 transcripts among 2667 and 2846 RPE cells. Dimensional reduction by principal component analysis and uniform manifold approximation and projection revealed six distinct cell populations. All clusters expressed transcripts typical of RPE cells; the smallest (C1, containing 1–2% of total cells) exhibited the hallmarks of stem and/or progenitor (SP) cells. Placing C1–6 along a pseudotime axis suggested a relative decrease in melanogenesis and SP gene expression and a corresponding increase in visual cycle gene expression upon RPE maturation. K-means clustering of all detected transcripts identified additional expression patterns that may advance the understanding of RPE SP cell maintenance and the evolution of cellular metabolic networks during development. This work provides new insights into the transcriptome of the mouse RPE and a baseline for identifying experimentally induced transcriptional changes in future studies of this tissue.
Epigenetic Dynamics and Regulation of Plant Male Reproduction
Authors: Hou Quancan , Zhang Tianye , Qi Yuchen , Dong Zhenying , Wan Xiangyuan
Published: September 2022

Flowering plant male germlines develop within anthers and undergo epigenetic reprogramming with dynamic changes in DNA methylation, chromatin modifications, and small RNAs. Profiling the epigenetic status using different technologies has substantially accumulated information on specific types of cells at different stages of male reproduction. Many epigenetically related genes involved in plant gametophyte development have been identified, and the mutation of these genes often leads to male sterility. Here, we review the recent progress on dynamic epigenetic changes during pollen mother cell differentiation, microsporogenesis, microgametogenesis, and tapetal cell development. The reported epigenetic variations between male fertile and sterile lines are summarized. We also summarize the epigenetic regulation-associated male sterility genes and discuss how epigenetic mechanisms in plant male reproduction can be further revealed.
Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Accelerate Diabetic Wound Healing via Promoting M2 Macrophage Polarization, Angiogenesis, and Collagen Deposition
Authors: Teng Liping , Maqsood Maria , Zhu Min , Zhou Yuting , Kang Mingzhu , Zhou Juan , Chen Jinghua
Published: September 2022

Some scholars have suggested that the clinical application of exosomes derived from human umbilical cord mesenchymal stem cells (hucMSCs-exo) might represent a novel strategy to improve diabetic wound healing. However, the mechanisms underlying the effects of hucMSCs-exo on wound healing remain poorly understood. This study aimed to identify the mechanism of hucMSCs-exo in treating diabetic wounds. HucMSCs-exo were isolated from human umbilical cord mesenchymal stem cells (hucMSCs) and subcutaneously injected into full-thickness wounds in diabetic rats. Wound healing closure rates and histological analysis were performed. The levels of tumor necrosis factor-α (TNF-α), macrophage mannose receptor (MMR/CD206), platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31), and vascular endothelial growth factor (VEGF) were detected by immunohistochemistry. The degree of collagen deposition was examined using Masson’s trichrome staining. Gross evaluation of wound healing was carried out from day 0 to 14 post-surgery, and the wound site was harvested for histology on days 3, 7, and 14 post-wounding. HucMSCs-exo transplantation increased diabetic wound healing. In vitro, hucMSCs-exo promoted the proliferation of human umbilical vein endothelial cells (HUVECs) and NIH-3T3 cells. In vivo, hucMSCs-exo reduced wound area and inflammatory infiltration and increased collagen fibers. In addition, wound tissues in the hucMSCs-exo group had higher CD206, CD31, and VEGF expressions and lower TNF-α levels than those in the control group on day 14. Our results demonstrated that hucMSCs-exo facilitated diabetic wound repair by inducing anti-inflammatory macrophages and promoting angiogenesis and collagen deposition.
Inflammatory Biomarkers Associated with In-Hospital Mortality in Critical COVID-19 Patients
Authors: Pál Krisztina , Molnar Anca Alexandra , Huțanu Adina , Szederjesi János , Branea Ionuț , Timár Ágota , Dobreanu Minodora
Published: September 2022

The COVID-19 pandemic poses global healthcare challenges due to its unpredictable clinical course. The aim of this study is to identify inflammatory biomarkers and other routine laboratory parameters associated with in-hospital mortality in critical COVID-19 patients. We performed a retrospective observational study on 117 critical COVID-19 patients. Following descriptive statistical analysis of the survivor and non-survivor groups, optimal cut-off levels for the statistically significant parameters were determined using the ROC method, and the corresponding Kaplan-Meier survival curves were calculated. The inflammatory parameters that present statistically significant differences between survivors and non-survivors are IL-6 (p = 0.0004, cut-off = 27.68 pg/mL), CRP (p = 0.027, cut-off = 68.15 mg/L) and IL-6/Ly ratio (p = 0.0003, cut-off = 50.39). Additionally, other statistically significant markers are creatinine (p = 0.031, cut-off = 0.83 mg/dL), urea (p = 0.0002, cut-off = 55.85 mg/dL), AST (p = 0.0209, cut-off = 44.15 U/L), INR (p = 0.0055, cut-off = 1.075), WBC (p = 0.0223, cut-off = 11.68 × 109/L) and pH (p = 0.0055, cut-off = 7.455). A survival analysis demonstrated significantly higher in-hospital mortality rates of patients with values of IL-6, IL-6/Ly, AST, INR, and pH exceeding previously mentioned thresholds. In our study, IL-6 and IL-6/Ly have a predictive value for the mortality of critically-ill patients diagnosed with COVID-19. The integration of these parameters with AST, INR and pH could contribute to a prognostic score for the risk stratification of critical patients, reducing healthcare costs and facilitating clinical decision-making.
Modification of Polymer Based Dentures on Biological Properties: Current Update, Status, and Findings
Authors: Mohd Farid Durratul Aqwa , Zahari Nur A’fifah Husna , Said Zulfahmi , Ghazali Mohd Ifwat Mohd , Hao-Ern Lee , Mohamad Zol Syazwani , Aldhuwayhi Sami , Alauddin Muhammad Syafiq
Published: September 2022

Polymers remain an integral part of denture fabrication materials, specifically polymethylmetacrylate (PMMA). PMMA has been extensively used, particularly in construction as a denture base material. Nonetheless, various challenges, including microbial threats in the form of candidiasis occurrence, still remain a biological challenge to denture wearers. The present article comprehensively reviews the biomodifications introduced to denture components, in particular denture base material, to improve the overall biological properties, together with physical, mechanical, structural integrity, and optical properties. In addition, fundamental information specifically to PMMA as a conventional denture base material and the causative aetiological microbial agents for biological threat to dentures are explored.
Distinct Responses to Pathogenic and Symbionic Microorganisms: The Role of Plant Immunity
Authors: Ji Li , Yang Xiangrui , Qi Feifei
Published: September 2022

Plants must balance both beneficial (symbiotic) and pathogenic challenges from microorganisms, the former benefitting the plant and agriculture and the latter causing disease and economic harm. Plant innate immunity describes a highly conserved set of defense mechanisms that play pivotal roles in sensing immunogenic signals associated with both symbiotic and pathogenic microbes and subsequent downstream activation of signaling effector networks that protect the plant. An intriguing question is how the innate immune system distinguishes “friends” from “foes”. Here, we summarize recent advances in our understanding of the role and spectrum of innate immunity in recognizing and responding to different microbes. In addition, we also review some of the strategies used by microbes to manipulate plant signaling pathways and thus evade immunity, with emphasis on the use of effector proteins and micro-RNAs (miRNAs). Furthermore, we discuss potential questions that need addressing to advance the field of plant–microbe interactions.
SARS-CoV-2 Spike Proteins and Cell–Cell Communication Inhibits TFPI and Induces Thrombogenic Factors in Human Lung Microvascular Endothelial Cells and Neutrophils: Implications for COVID-19 Coagulopathy Pathogenesis
Authors: Bhargavan Biju , Kanmogne Georgette D.
Published: September 2022

In SARS-CoV-2-infected humans, disease progression is often associated with acute respiratory distress syndrome involving severe lung injury, coagulopathy, and thrombosis of the alveolar capillaries. The pathogenesis of these pulmonary complications in COVID-19 patients has not been elucidated. Autopsy study of these patients showed SARS-CoV-2 virions in pulmonary vessels and sequestrated leukocytes infiltrates associated with endotheliopathy and microvascular thrombosis. Since SARS-CoV-2 enters and infects target cells by binding its spike (S) protein to cellular angiotensin-converting enzyme 2 (ACE2), and there is evidence that vascular endothelial cells and neutrophils express ACE2, we investigated the effect of S-proteins and cell–cell communication on primary human lung microvascular endothelial cells (HLMEC) and neutrophils expression of thrombogenic factors and the potential mechanisms. Using S-proteins of two different SARS-CoV-2 variants (Wuhan and Delta), we demonstrate that exposure of HLMEC or neutrophils to S-proteins, co-culture of HLMEC exposed to S-proteins with non-exposed neutrophils, or co-culture of neutrophils exposed to S-proteins with non-exposed HLMEC induced transcriptional upregulation of tissue factor (TF), significantly increased the expression and secretion of factor (F)-V, thrombin, and fibrinogen and inhibited tissue factor pathway inhibitor (TFPI), the primary regulator of the extrinsic pathway of blood coagulation, in both cell types. Recombinant (r)TFPI and a thiol blocker (5,5′-dithio-bis-(2-nitrobenzoic acid)) prevented S-protein-induced expression and secretion of Factor-V, thrombin, and fibrinogen. Thrombomodulin blocked S-protein-induced expression and secretion of fibrinogen but had no effect on S-protein-induced expression of Factor-V or thrombin. These results suggests that following SARS-CoV-2 contact with the pulmonary endothelium or neutrophils and endothelial–neutrophil interactions, viral S-proteins induce coagulopathy via the TF pathway and mechanisms involving functional thiol groups. These findings suggest that using rTFPI and/or thiol-based drugs could be a viable therapeutic strategy against SARS-CoV-2-induced coagulopathy and thrombosis.
A Cognitive Model to Anticipate Variations of Situation Awareness and Attention for the Takeover in Highly Automated Driving
Authors: Scharfe-Scherf Marlene Susanne Lisa , Wiese Sebastian , Russwinkel Nele
Published: September 2022

The development of highly automated driving requires dynamic approaches that anticipate the cognitive state of the driver. In this paper, a cognitive model is developed that simulates a spectrum of cognitive processing and the development of situation awareness and attention guidance in different takeover situations. In order to adapt cognitive assistance systems according to individuals in different situations, it is necessary to understand and simulate dynamic processes that are performed during a takeover. To validly represent cognitive processing in a dynamic environment, the model covers different strategies of cognitive and visual processes during the takeover. To simulate the visual processing in detail, a new module for the visual attention within different traffic environments is used. The model starts with a non-driving-related task, attends the takeover request, makes an action decision and executes the corresponding action. It is evaluated against empirical data in six different driving scenarios, including three maneuvers. The interaction with different dynamic traffic scenarios that vary in their complexity is additionally represented within the model. Predictions show variances in reaction times. Furthermore, a spectrum of driving behavior in certain situations is represented and how situation awareness is gained during the takeover process. Based on such a cognitive model, an automated system could classify the driver’s takeover readiness, derive the expected takeover quality and adapt the cognitive assistance for takeovers accordingly to increase safety.
Toxicity, Behavioral Effects, and Chitin Structural Chemistry of Reticulitermes flaviceps Exposed to Cymbopogon citratus EO and Its Major Constituent Citral
Authors: Jin Chunzhe , Han Hui , Xie Yongjian , Li Baoling , Zhang Zhilin , Zhang Dayu
Published: September 2022

Botanical pesticides are considered the most promising alternative to synthetic pesticides, considering their less negative impacts on the environment and human health. Here, we analyzed the components of lemongrass Cymbopogon citratus essential oil (EO) and evaluated its vapor activity against Reticulitermes flaviceps, in terms of the walking and gripping abilities of workers. In addition, the effects of lemongrass EO and its major component on the cuticular content and structure of chitin in termites were also observed. Our results indicate that cis-citral (36.51%) was the main constituent of lemongrass. In the vapor toxicity assay, the LC50 values of lemongrass EO and citral were 0.328 and 0.177 μL/L, respectively. When worker antennae were treated with lemongrass EO and citral, their walking and gripping capabilities were significantly inhibited. In addition, the cuticular content, thermal stability, and crystallinity of chitin in the termites were decreased after treatment with citral. Collectively, this study provides a basis for developing and utilizing lemongrass and citral as a new environment-friendly insecticide resource to control R. flaviceps.
Motorized Intramedullary Nail Lengthening in the Older Population
Authors: Powell Kenneth P. , Hammouda Ahmed I. , Hlukha Larysa P. , Rivera Jessica C. , Patel Minoo , Rozbruch S. Robert , Conway Janet D. , Herzenberg John E.
Published: September 2022

Limb lengthening has not been widely employed in the elderly population due to concerns that outcomes will be inferior. The purpose of this multicenter, retrospective case-control series was to report the bone healing outcomes and complications of lower limb lengthening in older patients (≥60 years) using magnetic intramedullary lengthening nail (MILN). Our hypothesis was that healing parameters including consolidation days, the consolidation index, maturation days, and the maturation index, as well as the number of adverse events reported in the older population, would be no different to those of the general adult population. We retrospectively reviewed charts and radiographs from patients ≥60 years of age with limb-length discrepancies who underwent femoral or tibial lengthening using a MILN. Parameters were compared among the age categories “≤19 years,” “20–39 years,” “40–59 years,” or “≥60 years” and propensity-matched cohorts for the age groups 20–59 years and ≥60 years. Complications were reported as percentages for each age category. In the study period, 354 MILN were placed in 257 patients. Sixteen nails were placed in patients 60 years of age or older (mean 65 ± 5 years; range 60–72 years). Comparisons of healing parameters showed no difference between those aged 60+ and the younger cohort. Complication percentages were not statistically significant (p = 0.816). Limb lengthening with MILN may therefore be considered a safe and feasible option for a generally healthy elderly population.
Compromised Teeth Preserve or Extract: A Review of the Literature
Authors: Cárcamo-España Valentina , Cuesta Reyes Nataly , Flores Saldivar Paul , Chimenos-Küstner Eduardo , Estrugo Devesa Alberto , López-López José
Published: September 2022

Multiple systems and associated factors have been described in the literature to assess the prognosis of teeth with periodontal disease. Nowadays there is a tendency among clinicians to consider implants as the best solution after tooth extraction, in cases of teeth with a questionable prognosis. However, the value of the natural tooth must be considered, as the proprioception of the periodontal ligament is preserved, and it adapts to stress during functional loads. We first review the literature focusing on analyzing the factors that should guide decision-making to maintain or extract a tooth with a compromised periodontium. Then, we propose a schematic diagram of prognostic indicators to reflect the main factors to consider and the survival rate that each one represents when preserving or extracting a tooth.
Ozone Infiltration for Osteonecrosis of the Jaw Therapy: A Case Series
Authors: Di Fede Olga , Del Gaizo Carmine , Panzarella Vera , La Mantia Gaetano , Tozzo Pietro , Di Grigoli Anna , Lo Casto Antonio , Mauceri Rodolfo , Campisi Giuseppina
Published: September 2022

Medication-related osteonecrosis of the jaw (MRONJ) is a serious adverse reaction, mainly due to bone-modifying agents (BMA), and it is a potentially painful and debilitating condition. To date, the literature has reported a 90% rate of successful outcomes for MRONJ patients undergoing surgical treatment. Particularly for patients with advanced disease stages who are unsuitable for surgery, prolonged medical treatment is required, with a consequent risk of the overuse of antibiotics and antibiotic resistance. The aim of this study is to evaluate the efficiency and safety of ozone, via oral mucosal infiltrations, in seven cancer patients with MRONJ, who are not eligible for surgery. The protocol (OZOPROMAF) consists of intratissue injections of an oxygen ozone (O2O3) mixture, which is applied until formation of a sequestrum and clinical healing. Follow-up was scheduled to confirm the healing of MRONJ and radiological evaluations by CBCT were planned. In order to assess the level of pain, a questionnaire including the Numeric Rating Scale for Pain (NRS Pain) was administered on the first visit, one day after treatment, and one week after treatment. After an application of OZOPROMAF, all patients reported discomfort for some hours, probably due to soft tissue pressure around the infiltration site. Thereafter, the discomfort subsided within 6–8 h. Complete mucosal healing of MRONJ occurred within a number of cycles ranging from 7 to 16. Complete resolution with an improvement in bone condition was observed in all patients. The MRONJ lesions of all patients healed after 18–24 months. The authors of this study contend that these preliminary results suggest the efficiency and safety of the O2O3 mixture. However, further research is required to confirm the efficacy of the O2O3 mixtures in MRONJ treatment, at least for patients who are unsuitable for surgery.
Impact of COVID-19 Pandemic on Initiation of Immunosuppressive Treatment in Immune-Mediated Inflammatory Diseases in Austria: A Nationwide Retrospective Study
Authors: Kutschera Maximilian , Ritschl Valentin , Reichardt Berthold , Stamm Tanja , Kiener Hans , Maier Harald , Reinisch Walter , Benka Bernhard , Novacek Gottfried
Published: September 2022

Objective: Conventional immunosuppressive and advanced targeted therapies, including biological medications and small molecules, are a mainstay in the treatment of immune-mediated inflammatory diseases (IMID). However, the COVID-19 pandemic caused concerns over these drugs’ safety regarding the risk and severity of SARS-CoV-2 infection. Thus, we aimed to assess the impact of the COVID-19 pandemic on the initiation of these treatments in 2020. Study Design and Setting: We conducted a population-based retrospective analysis of real-world data of the Austrian health insurance funds on the initiation of conventional immunosuppressive and advanced targeted therapies. The primary objective was to compare the initiation of these medications in the year 2020 with the period 2017 to 2019. Initiation rates of medication were calculated by comparing a certain unit of time with an average of the previous ones. Results: 95,573 patients were included. During the first lockdown in Austria in April 2020, there was a significant decrease in the initiations of conventional immunosuppressives and advanced targeted therapies compared to previous years (p < 0.0001). From May 2020 onwards, numbers rapidly re-achieved pre-lockdown levels despite higher SARS-CoV-2 infection rates and subsequent lockdown periods at the end of 2020. Independent from the impact of the COVID-19 pandemic, a continuous increase of starts of advanced targeted therapies and a continuous decrease of conventional immunosuppressants during the observation period were observed. Conclusions: In IMID patients, the COVID-19 pandemic led to a significant decrease of newly started conventional immunosuppressive and advanced targeted therapies only during the first lockdown in Austria.