bluuebunny/crossref_metadata_2025 huggingface dataset:
Near-Infrared Spectroscopy (NIRS) as a Tool for Classification of Pre-Sliced Iberian Salchichón, Modified Atmosphere Packaged (MAP) According to the Official Commercial Categories of Raw Meat
Authors: Ortiz Alberto , León Lucía , Contador Rebeca , Tejerina David
Published: August 2021

This study evaluates near-infrared spectroscopy (NIRS) feasibility in combination with various pre-treatments and chemometric approaches for pre-sliced Iberian salchichón under modified atmosphere (MAP) classification according to the official commercial category (defined by the combination of genotype and feeding regime) of the raw material used for its manufacturing (Black and Red purebred Iberian and Iberian × Duroc crossed (50%) pigs, respectively, reared outdoors in a Montanera system and White Iberian × Duroc crossed (50%) pigs with feed based on commercial fodder) without opening the package. In parallel, NIRS feasibility in combination with partial least squares regression (PLSR) to predict main quality traits was assessed. The best-fitting models developed by means of partial least squares discriminant analysis (PLS-DA) and linear discriminant analysis (LDA) yielded high discriminant ability and thus offered a tool to support the assignment of pre-sliced MAP Iberian salchichón according to the commercial category of the raw material. In addition, good predictive ability for C18:3 n-3 was obtained, which may help to support quality control.
Acidic and Electrosurface Properties of Binary TiO2-SiO2 Xerogels Using EPR of pH-Sensitive Nitroxides
Authors: Antonov Denis O. , Tambasova Daria P. , Shishmakov Andrey B. , Kirilyuk Igor A. , Kovaleva Elena G.
Published: August 2021

The binary xerogels TiO2-SiO2 are widely used as catalysts and their carriers in organic synthesis. Characterization and adjustment of the electrostatic properties of the surface and the local acidity inside the pores, are necessary for the further development of TiO2-SiO2 xerogels applications. This research investigates acid–base equilibria in the pores, and the surface electrostatic potential (SEP) of binary TiO2-SiO2 xerogels, by the EPR of stable pH-sensitive nitroxide radicals. These radicals are small enough to penetrate directly into the pores, and to be adsorbed onto the surface of the material under study. This makes it possible to obtain valuable information on the acidic and electrosurface properties of the studied system. The highest negative surface electrical charge associated with surface electrical potential (SEP) was equal to −196 ± 6 mV. It was induced by the surface of the sample with a 7% TiO2 content. The local acidity inside the pores of this sample was found to be higher, by approximately 1.49 pH units, as compared to that in the external bulk solution.
QTL Genetic Mapping Study for Traits Affecting Meal Quality in Winter Oilseed Rape (Brassica Napus L.)
Authors: Gacek Katarzyna , Bayer Philipp E. , Anderson Robyn , Severn-Ellis Anita A. , Wolko Joanna , Łopatyńska Agnieszka , Matuszczak Marcin , Bocianowski Jan , Edwards David , Batley Jacqueline
Published: August 2021

Rapeseed (Brassica napus L.) meal is an important source of protein, but the presence of anti-nutritional compounds, such as fibre and glucosinolates, still limits its use as a livestock feed. Understanding the genetic basis of seed fibre biosynthesis would help to manipulate its content in seeds of oilseed rape. Here, we applied high-resolution skim genotyping by sequencing (SkimGBS) and characterised 187,835 single-nucleotide polymorphism (SNP) markers across a mapping population subsequently used for a genetic mapping study (R/qtl). This approach allowed the identification of 11 stable QTL related to seed quality traits and led to the identification of potential functional genes underlying these traits. Among these, key genes with a known role in carbohydrate metabolic process, cell wall, lignin, and flavonoid biosynthesis, including cellulase GH5, TT10/LAC15, TT4, and SUC2, were found. This study furthers the understanding of the molecular mechanisms underlying seed fibre content and provides new markers for molecular breeding in B. napus.
Diversity and Paleodemography of the Addax (Addax nasomaculatus), a Saharan Antelope on the Verge of Extinction
Authors: Hempel Elisabeth , Westbury Michael V. , Grau José H. , Trinks Alexandra , Paijmans Johanna L. A. , Kliver Sergei , Barlow Axel , Mayer Frieder , Müller Johannes , Chen Lei , Koepfli Klaus-Peter , Hofreiter Michael , Bibi Faysal
Published: August 2021

Since the 19th century, the addax (Addax nasomaculatus) has lost approximately 99% of its former range. Along with its close relatives, the blue antelope (Hippotragus leucophaeus) and the scimitar-horned oryx (Oryx dammah), the addax may be the third large African mammal species to go extinct in the wild in recent times. Despite this, the evolutionary history of this critically endangered species remains virtually unknown. To gain insight into the population history of the addax, we used hybridization capture to generate ten complete mitochondrial genomes from historical samples and assembled a nuclear genome. We found that both mitochondrial and nuclear diversity are low compared to other African bovids. Analysis of mitochondrial genomes revealed a most recent common ancestor ~32 kya (95% CI 11–58 kya) and weak phylogeographic structure, indicating that the addax likely existed as a highly mobile, panmictic population across its Sahelo–Saharan range in the past. PSMC analysis revealed a continuous decline in effective population size since ~2 Ma, with short intermediate increases at ~500 and ~44 kya. Our results suggest that the addax went through a major bottleneck in the Late Pleistocene, remaining at low population size prior to the human disturbances of the last few centuries.
Improvement the Healthcare Quality of Emergency Department after the Cloud-Based System of Medical Information-Exchange Implementation
Authors: Wu Ding-Chung , Lin Hong-Ling , Cheng Chun-Gu , Yu Chia-Peng , Cheng Chun-An
Published: August 2021

Background: The National Health Insurance has been implemented in Taiwan since 1995. The government established a medical information-exchange system to reduce duplicate medications and examinations, which have inhibited healthcare expenditures. The potential benefit of medical information exchange about healthcare quality in emergency departments (ED) was worthy of evaluating; Methods: The inquiry rate of cloud data for patients’ information in Taiwanese National Health Insurance Administration was defined as a factor, and the healthcare quality included the ratio of staying more than 48 h in the ED and the hospitalization rate within 8 h from ED by triage levels of 1, 2, and 3 in different levels of hospitals from 2013 to 2019. Poisson regression analysis was used to quantify time trends of the query rate of the MediCloud system, the rate of staying more than 48 h in ED, admission rate within 8 h in ED, and the effect of healthcare quality in ED after MediCloud system implementation; Results: The health information exchange decreased the rate of staying over 48 h in the ED of medical centers. It also improved the early hospitalization of urgent ED patients in regional hospitals; Conclusions: Through medical information exchange to understand patients’ current conditions, we can reduce crowding in the ED of medical centers and facilitate rapid hospitalization of urgent patients in regional hospitals. According to these findings, the government should establish medical information exchange to improve the healthcare quality of ED.
COVID-19 Vaccine Literacy of Family Carers for Their Older Parents in Japan
Authors: Costantini Hiroko
Published: August 2021

In super-ageing Japan, COVID-19 vaccinations were starting to reach older people as of June 2021, which raises the issue of vaccine literacy. This study focuses on family members who work and also care for their older parents, as they are at risk of COVID-19 and also risk transmitting COVID-19 to the parents they care for and potentially influencing their parents’ vaccine uptake. Such family carers are central to the approach in Japan to achieving a sustainable and resilient society in response to ageing. Contrasting family carers’ COVID-19 vaccine literacy with their overall health literacy provides insights into their preparedness for COVID-19 vaccinations. The purpose of this study is to understand how vaccine literacy, compared to health literacy, varies across family carers and the sources of information they use. Through a cross-sectional online survey, family carers’ vaccine literacy, health literacy and their sources of information, including mass media, social media, health and care professionals, family, colleagues, friends, and others, were assessed. The participants’ (n = 292) mean age was 53, with 44% women, and an average of 8.3 h per week caring for their parents. Notwithstanding the increased risks from COVID-19 with age, COVID-19 vaccine literacy relative to health literacy for older family carers is lower on average, higher with increased provision of care, and more variable, resulting in a substantial proportion of older family carers with relatively low vaccine literacy. At this stage of vaccine rollout in Japan, family carers’ sources of information to inform COVID-19 vaccine literacy is distinct, including more national and local mass media versus less health and care professionals and informal networks, which indicates the importance of tailored health communication strategies to enhance vaccine literacy
Does Self-Perception Equal the Truth When Judging Own Body Weight and Height?
Authors: Haakstad Lene A. H. , Stensrud Trine , Gjestvang Christina
Published: August 2021

Background: Data from the research project “Fitness clubs—a venue for public health?” provided an opportunity to evaluate the accuracy of self-reported body weight and height, and subsequent Body Mass Index (BMI), as well as the “trueness” of novice exercisers perception of weight status category, which has not been examined in this population. The aims were to examine self-reported body weight, height, and calculated BMI data from an online survey compared with measured data at fitness club start-up, investigate how accurately novice exercisers place themselves within self-classified weight group (underweight, normal weight, overweight, and obese), and compare this with fitness club attendance at three months follow-up. Methods: Prior to anthropometric measurements, 62 men and 63 women responded to an online questionnaire, including body weight (kilogram, kg) and height (centimeters, cm), and self-classified weight group (“I think I am … underweight, normal weight, overweight, obese”). We used the following statistical analysis: Paired sample t-tests, a Bland–Altman plot kappa statistics, chi-squared tests, and a logistic regression. Results: Mean difference of BMI calculated from self-reported and measured data was 0.06 (95% CI −0.29 to 0.17, p = 0.593) in men, and 0.16 (95% CI −0.40 to 0.09, p = 0.224) in women, with four participants being outliers of the 95% limits of agreement (Bland-Altman plot). Allowing a difference of 0.5 kg between self-reported and measured weight, we found that 16% reported their weight correctly, 31.2% underreported (−1.89 ± 1.59 kg), and 52.8% overreported (1.85 ± 1.23 kg), with no sex differences (p = 0.870). Further, our results suggest that both sexes may have difficulty recognizing overweight/obesity in themselves, and particularly men are likely to underreport their perceived weight group compared with women. More than half (53.3%) of the overweight men perceived themselves to be normal weight (women: 14%), and only 33.3% of obese men and women correctly classified themselves as being obese. We did not find any difference between participants correctly or incorrectly classifying weight group and fitness club attendance (≥2 times a week) at three months follow-up. Conclusion: Both sexes reported body weight and height reasonably accurately, and BMI based on self-report appears to be valid measure. Still, a large proportion of novice exercisers do not recognise their own overweight or obesity status, which may in part explain why public health campaigns do not reach risk populations.
Optimizing the Expression of Human Dopamine Receptors in Escherichia coli
Authors: Boritzki Vanessa , Hübner Harald , Allikalt Anni , Gmeiner Peter , Wöhrl Birgitta M.
Published: August 2021

The human dopamine receptors D2S and D3 belong to the group of G protein-coupled receptors (GPCRs) and are important drug targets. Structural analyses and development of new receptor subtype specific drugs have been impeded by low expression yields or receptor instability. Fusing the T4 lysozyme into the intracellular loop 3 improves crystallization but complicates conformational studies. To circumvent these problems, we expressed the human D2S and D3 receptors in Escherichia coli using different N- and C-terminal fusion proteins and thermostabilizing mutations. We optimized expression times and used radioligand binding assays with whole cells and membrane homogenates to evaluate KD-values and the number of receptors in the cell membrane. We show that the presence but not the type of a C-terminal fusion protein is important. Bacteria expressing receptors capable of ligand binding can be selected using FACS analysis and a fluorescently labeled ligand. Improved receptor variants can thus be generated using error-prone PCR. Subsequent analysis of clones showed the distribution of mutations over the whole gene. Repeated cycles of PCR and FACS can be applied for selecting highly expressing receptor variants with high affinity ligand binding, which in the future can be used for analytical studies.
Protective Effects of Flavonoids Against Mitochondriopathies and Associated Pathologies: Focus on the Predictive Approach and Personalized Prevention
Authors: Koklesova Lenka , Liskova Alena , Samec Marek , Zhai Kevin , AL-Ishaq Raghad Khalid , Bugos Ondrej , Šudomová Miroslava , Biringer Kamil , Pec Martin , Adamkov Marian , Hassan Sherif T. S. , Saso Luciano , Giordano Frank A. , Büsselberg Dietrich , Kubatka Peter , Golubnitschaja Olga
Published: August 2021

Multi-factorial mitochondrial damage exhibits a “vicious circle” that leads to a progression of mitochondrial dysfunction and multi-organ adverse effects. Mitochondrial impairments (mitochondriopathies) are associated with severe pathologies including but not restricted to cancers, cardiovascular diseases, and neurodegeneration. However, the type and level of cascading pathologies are highly individual. Consequently, patient stratification, risk assessment, and mitigating measures are instrumental for cost-effective individualized protection. Therefore, the paradigm shift from reactive to predictive, preventive, and personalized medicine (3PM) is unavoidable in advanced healthcare. Flavonoids demonstrate evident antioxidant and scavenging activity are of great therapeutic utility against mitochondrial damage and cascading pathologies. In the context of 3PM, this review focuses on preclinical and clinical research data evaluating the efficacy of flavonoids as a potent protector against mitochondriopathies and associated pathologies.
Genome-Wide Identification and Transcriptional Expression Analysis of Annexin Genes in Capsicum annuum and Characterization of CaAnn9 in Salt Tolerance
Authors: Wu Xiaoxia , Ren Yan , Jiang Hailong , Wang Yan , Yan Jiaxing , Xu Xiaoying , Zhou Fucai , Ding Haidong
Published: August 2021

Annexin (Ann) is a polygenic, evolutionarily conserved, calcium-dependent and phospholipid-binding protein family, which plays key roles in plant growth, development, and stress response. However, a comprehensive understanding of CaAnn genes of pepper (Capsicum annuum) at the genome-wide level is limited. Based on the available pepper genomic information, we identified 15 members of the CaAnn gene family. Phylogenetic analysis showed that CaAnn proteins could be categorized into four different orthologous groups. Real time quantitative RT-PCR analysis showed that the CaAnn genes were tissue-specific and were widely expressed in pepper leaves after treatments with cold, salt, and drought, as well as exogenously applied MeJA and ABA. In addition, the function of CaAnn9 was further explored using the virus-induced gene silencing (VIGS) technique. CaAnn9-silenced pepper seedlings were more sensitive to salt stress, reflected by the degradation of chlorophyll, the accumulation of reactive oxygen species (ROS), and the decrease of antioxidant defense capacity. This study provides important information for further study of the role of pepper CaAnn genes and their coding proteins in growth, development, and environmental responses.
Zn Promoted Mg-Al Mixed Oxides-Supported Gold Nanoclusters for Direct Oxidative Esterification of Aldehyde to Ester
Authors: Li Jie , Wang Shiyi , Li Huayin , Tan Yuan , Ding Yunjie
Published: August 2021

The synthesis of ester compounds is one of the most important chemical processes. In this work, Zn-Mg-Al mixed oxides with different Zn2+/Mg2+ molar ratios were prepared via co-precipitation method and supported gold nanoclusters to study the direct oxidative esterification of aldehyde and alcohol in the presence of molecular oxygen. Various characterization techniques such as N2-physical adsorption, X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and CO2 temperature programmed desorption (TPD) were utilized to analyze the structural and electronic properties. Based on the results, the presence of small amounts of Zn2+ ions (~5 wt.%) provoked a remarkable modification of the binary Mg-Al system, which enhanced the interaction between gold with the support and reduced the particle size of gold. For oxidative esterification reaction, the Au25/Zn0.05MgAl-400 catalyst showed the best performance, with the highest turnover frequency (TOF) of 1933 h−1. The active center was believed to be located at the interface between metallic gold with the support, where basic sites contribute a lot to transformation of the substrate.
Syndecan Family Gene and Protein Expression and Their Prognostic Values for Prostate Cancer
Authors: Santos Nilton José , Barquilha Caroline Nascimento , Barbosa Isabela Correa , Macedo Rodrigo Tavares , Lima Flávio Oliveira , Justulin Luis Antônio , Barbosa Guilherme Oliveira , Carvalho Hernandes F. , Felisbino Sérgio Luis
Published: August 2021

Prostate cancer (PCa) is the leading cause of cancer-associated mortality in men, and new biomarkers are still needed. The expression pattern and protein tissue localization of proteoglycans of the syndecan family (SDC 1–4) and syntenin-1 (SDCBP) were determined in normal and prostatic tumor tissue from two genetically engineered mouse models and human prostate tumors. Studies were validated using SDC 1–4 and SDCBP mRNA levels and patient survival data from The Cancer Genome Atlas and CamCAP databases. RNAseq showed increased expression of Sdc1 in Pb-Cre4/Ptenf/f mouse Pca and upregulation of Sdc3 expression and downregulation of Sdc2 and Sdc4 when compared to the normal prostatic tissue in Pb-Cre4/Trp53f/f-;Rb1f/f mouse tumors. These changes were confirmed by immunohistochemistry. In human PCa, SDC 1–4 and SDCBP immunostaining showed variable localization. Furthermore, Kaplan–Meier analysis showed that patients expressing SDC3 had shorter prostate-specific survival than those without SDC3 expression (log-rank test, p = 0.0047). Analysis of the MSKCC-derived expression showed that SDC1 and SDC3 overexpression is predictive of decreased biochemical recurrence-free survival (p = 0.0099 and p = 0.045, respectively), and SDC4 overexpression is predictive of increased biochemical recurrence-free survival (p = 0.035). SDC4 overexpression was associated with a better prognosis, while SDC1 and SDC3 were associated with more aggressive tumors and a worse prognosis.
Functionalization of Human Serum Albumin by Tyrosine Click
Authors: Obara Satsuki , Nakane Keita , Fujimura Chizu , Tomoshige Shusuke , Ishikawa Minoru , Sato Shinichi
Published: August 2021

Human serum albumin (HSA) is a promising drug delivery carrier. Although covalent modification of Cys34 is a well-established method, it is desirable to develop a novel covalent modification method that targets residues other than cysteine to introduce multiple functions into a single HSA molecule. We developed a tyrosine-selective modification of HSA. Three tyrosine selective modification methods, hemin-catalyzed, horseradish peroxidase (HRP)-catalyzed, and laccase-catalyzed reactions were performed, and the modification efficiencies and modification sites of the modified HSAs obtained by these methods were evaluated and compared. We found that the laccase-catalyzed method could efficiently modify the tyrosine residue of HSA under mild reaction conditions without inducing oxidative side reactions. An average of 2.2 molecules of functional groups could be introduced to a single molecule of HSA by the laccase method. Binding site analysis using mass spectrometry suggested Y84, Y138, and Y401 as the main modification sites. Furthermore, we evaluated binding to ibuprofen and found that, unlike the conventional lysine residue modification, the inhibition of drug binding was minimal. These results suggest that tyrosine-residue selective chemical modification is a promising method for covalent drug attachment to HSA.
T-Cell Responses in Merkel Cell Carcinoma: Implications for Improved Immune Checkpoint Blockade and Other Therapeutic Options
Authors: Gehrcken Laura , Sauerer Tatjana , Schaft Niels , Dörrie Jan
Published: August 2021

Merkel cell carcinoma (MCC) is a rare and aggressive skin cancer with rising incidence and high mortality. Approximately 80% of the cases are caused by the human Merkel cell polyomavirus, while the remaining 20% are induced by UV light leading to mutations. The standard treatment of metastatic MCC is the use of anti-PD-1/-PD-L1-immune checkpoint inhibitors (ICI) such as Pembrolizumab or Avelumab, which in comparison with conventional chemotherapy show better overall response rates and longer duration of responses in patients. Nevertheless, 50% of the patients do not respond or develop ICI-induced, immune-related adverse events (irAEs), due to diverse mechanisms, such as down-regulation of MHC complexes or the induction of anti-inflammatory cytokines. Other immunotherapeutic options such as cytokines and pro-inflammatory agents or the use of therapeutic vaccination offer great ameliorations to ICI. Cytotoxic T-cells play a major role in the effectiveness of ICI, and tumour-infiltrating CD8+ T-cells and their phenotype contribute to the clinical outcome. This literature review presents a summary of current and future checkpoint inhibitor therapies in MCC and demonstrates alternative therapeutic options. Moreover, the importance of T-cell responses and their beneficial role in MCC treatment is discussed.
Bio-Morphological Reaction of Human Periodontal Ligament Fibroblasts to Different Types of Dentinal Derivates: In Vitro Study
Authors: Bianchi Serena , Mancini Leonardo , Torge Diana , Cristiano Loredana , Mattei Antonella , Varvara Giuseppe , Macchiarelli Guido , Marchetti Enrico , Bernardi Sara
Published: August 2021

Understanding the biological and morphological reactions of human cells towards different dentinal derivate grafting materials is fundamental for choosing the type of dentin for specific clinical situations. This study aimed to evaluate human periodontal ligament fibroblasts (hPLF) cells exposed to different dentinal derivates particles. The study design included the in vitro evaluation of mineralized dentine (SG), deproteinized and demineralized dentine (DDP), and demineralized dentine (TT) as test materials and of deproteinized bovine bone (BIOS) as the positive control material. The materials were kept with the hPLF cell line, and the evaluations were made after 24 h, 72 h, and 7 days of in vitro culture. The evaluated outcomes were proliferation by using XTT assays, the morphological characteristics by light microscopy (LM) and by the use of scanning electron microscopy (SEM), and adhesion by using confocal microscopy (CLSM). Overall, the experimental materials induced a positive response of the hPLFs in terms of proliferation and adhesion. The XTT assay showed the TT, and the SG induced significant growth compared to the negative control at 7 days follow-up. The morphological data supported the XTT assay: the LM observations showed the presence of densely packed cells with a modified shape; the SEM observations allowed the assessment of how fibroblasts exposed to DDP and TT presented cytoplasmatic extensions; and SG and BIOS also presented the thickening of the cellular membrane. The CLMS observations showed the expression of the proliferative marker, as well as and the expression of cytoskeletal elements involved in the adhesion process. In particular, the vinculin and integrin signals were stronger at 72 h, while the actin signal remained constantly expressed in all the follow-up of the sample exposed to SG material. The integrin signal was stronger at 72 h, and the vinculin and actin signals were stronger at 7 days follow-up in the sample exposed to DDP material. The vinculin and integrin signals were stronger at 72 h follow-up in the sample exposed to TT material; vinculin and integrin signals appear stronger at 24 h follow-up in the sample exposed to BIOS material. These data confirmed how dentinal derivates present satisfying biocompatibility and high conductivity and inductivity properties fundamental in the regenerative processes. Furthermore, the knowledge of the effects of the dentin’s degree of mineralization on cellular behavior will help clinicians choose the type of dentine derivates material according to the required clinical situation.
Role of Nuclear Factor of Activated T Cells (NFAT) Pathway in Regulating Autophagy and Inflammation in Retinal Pigment Epithelial Cells
Authors: Pan Hsuan-Yeh , Ladd Ashley V. , Biswal Manas R. , Valapala Mallika
Published: August 2021

Nuclear factor of activated T cells (NFAT) family of transcription factors are substrates of calcineurin and play an important role in integrating Ca2+ signaling with a variety of cellular functions. Of the five NFAT proteins (NFAT1-5), NFAT1-4 are subject to dephosphorylation and activation by calcineurin, a Ca2+-calmodulin-dependent phosphatase. Increased levels of intracellular Ca2+ activates calcineurin, which in turn dephosphorylates and promotes nuclear translocation of NFAT. We investigated the functions of NFAT proteins in the retinal pigment epithelial cells (RPE). Our results show that NFAT-mediated luciferase activity was induced upon treatment with the bacterial endotoxin, lipopolysaccharide (LPS) and treatment with the NFAT peptide inhibitor, MAGPHPVIVITGPHEE (VIVIT) decreased LPS-induced NFAT luciferase activity. LPS-induced activation of NFAT-regulated cytokines (IL-6 and IL-8) is inhibited by treatment of cells with VIVIT. We also investigated the effects of NFAT signaling on the autophagy pathway. Our results show that inhibition of NFAT with VIVIT in cells deprived of nutrients resulted in cytosolic retention of transcription Factor EB (TFEB), decreased expression of TFEB-regulated coordinated Lysosomal Expression and Regulation CLEAR network genes and decreased starvation-induced autophagy flux in the RPE cells. In summary, these studies suggest that the NFAT pathway plays an important role in the regulation of autophagy and inflammation in the RPE.
Plant NIGT1/HRS1/HHO Transcription Factors: Key Regulators with Multiple Roles in Plant Growth, Development, and Stress Responses
Authors: Li Qian , Zhou Luyan , Li Yuhong , Zhang Dongping , Gao Yong
Published: August 2021

The NIGT1/HRS1/HHO transcription factor (TF) family is a new subfamily of the G2-like TF family in the GARP superfamily and contains two conserved domains: the Myb-DNA binding domain and the hydrophobic and globular domain. Some studies showed that NIGT1/HRS1/HHO TFs are involved in coordinating the absorption and utilization of nitrogen and phosphorus. NIGT1/HRS1/HHO TFs also play an important role in plant growth and development and in the responses to abiotic stresses. This review focuses on recent advances in the structural characteristics of the NIGT1/HRS1/HHO TF family and discusses how the roles and functions of the NIGT1/HRS1/HHO TFs operate in terms of in plant growth, development, and stress responses.
Agent-Based Simulation Framework for Epidemic Forecasting during Hajj Seasons in Saudi Arabia
Authors: Alshammari Sultanah Mohammed , Ba-Aoum Mohammed Hassan , Alganmi Nofe Ateq , Allinjawi Arwa AbdulAziz
Published: August 2021

The religious pilgrimage of Hajj is one of the largest annual gatherings in the world. Every year approximately three million pilgrims travel from all over the world to perform Hajj in Mecca in Saudi Arabia. The high population density of pilgrims in confined settings throughout the Hajj rituals can facilitate infectious disease transmission among the pilgrims and their contacts. Infected pilgrims may enter Mecca without being detected and potentially transmit the disease to other pilgrims. Upon returning home, infected international pilgrims may introduce the disease into their home countries, causing a further spread of the disease. Computational modeling and simulation of social mixing and disease transmission between pilgrims can enhance the prevention of potential epidemics. Computational epidemic models can help public health authorities predict the risk of disease outbreaks and implement necessary intervention measures before or during the Hajj season. In this study, we proposed a conceptual agent-based simulation framework that integrates agent-based modeling to simulate disease transmission during the Hajj season from the arrival of the international pilgrims to their departure. The epidemic forecasting system provides a simulation of the phases and rituals of Hajj following their actual sequence to capture and assess the impact of each stage in the Hajj on the disease dynamics. The proposed framework can also be used to evaluate the effectiveness of the different public health interventions that can be implemented during the Hajj, including size restriction and screening at entry points.
A Resilience-Based Model for the Seismic Assessment of the Functionality of Road Networks Affected by Bridge Damage and Restoration
Authors: Rasulo Alessandro , Pelle Angelo , Briseghella Bruno , Nuti Camillo
Published: August 2021

Road network functionality after an earthquake is a crucial aspect for an already struck community. In particular, bridges are susceptible to earthquake-induced damages and to lengthy restoration works. This may lead to severe and unexpected disruption of traffic. In this paper, a model for the assessment of the seismic resilience of a road network is presented. The proposed model permits us to evaluate the earthquake-induced perturbations to the functionality of a network in terms of transportation capacities, traffic congestion, and travel times due to bridge damages and subsequent restoration interventions. The evolution over time of the functionality of the network is studied by means of a multi-stage approach describing the evolution of the situation in terms of reducing the normal pre-earthquakes transportation capacities. The methodology has been illustrated with reference to a hypothetical case study, a road network composed of 14 nodes and 31 links.
The Direct Cause of Amplified Wettability: Roughness or Surface Chemistry?
Authors: Ubuo Emmanuel E. , Udoetok Inimfon A. , Tyowua Andrew T. , Ekwere Ifiok O. , Al-Shehri Hamza S.
Published: August 2021

Higher contact angles or amplified wettability observed on surfaces of rough solid materials are typically expressed as a function of a physical dimension (roughness factor). Herein, we present a simple experimental approach that demonstrates that roughness may only magnify the inherent surface chemistry that seems to have direct influence on surface wettability. We investigate gradual change in surface chemistry (hydrophobisation) of rough and smooth glass surfaces, from a very low concentration (10−7 M) of dichlorodimethylsilane, DCDMS through various intermediate hydrophilic/hydrophobic states to when the surfaces are maximally hydrophobised with DCDMS at 0.1 M. The wettability of the modified glasses was studied by water contact angle measurements using drop shape analysis system (DSA). The data obtained indicate a deviation from Wenzel model, with the functionalized rough glass surfaces showing higher reactivity towards DCDMS when compared to the smooth glass surfaces, indicating that the two surfaces are not chemically identical. Our study reveals that just like transforming a solid material to powder, a well-divided glass (rough) surface may not only exhibit a greater surface area than the smooth counterpart as rightly predicted by the Wenzel model, but seems to be bloated with functional groups (–OH or –CH3) that can amplify surface interaction when such functional species dominate the solid surface.
Mechanical and Tribological Attributes of Al-CNT-Sn Composites Prepared by Press and Sintering
Authors: Dhore Vilas , Rathod Walmik , Patil Kashinath
Published: August 2021

Carbon nanotubes (CNTs) have shown tremendous progress during the past two decades due to their extraordinary properties. With CNTs added as an alloying element, various engineering materials exhibit better mechanical properties. Multi-walled carbon nanotubes (MWCNT) were synthesized in-house by chemical vapor deposition process. Carbon nanotube-reinforced aluminum composites were prepared by cold pressing (or compaction) and sintering using different fractions (0.5, 1.0, 1.5, and 2.0 weight percent) of MWCNTs. The Al-CNT composites consists of tin (Sn) at 1.0 wt. % in each composition. Tin promotes the sintering of aluminum matrix composite. The effect of CNT on the density, hardness, and wear behavior of the composites were studied. Wear tests were performed to determine friction and wear under dry, wet, and hot conditions under varying loads from 5 N to 20 N. X-ray diffraction, Raman spectroscopy, scanning electron microscopy, and transmission electron microscopy techniques were used for the characterization. This investigation shows that increased CNT content significantly improves the hardness and wear resistance of the composites. The friction and wear were found to increase with operating temperature. A significant reduction in coefficient of friction and wear rate was observed with the application of oil during the wear test.
Formulation and Characterization of a New Injectable Bone Substitute Composed PVA/Borax/CaCO3 and Demineralized Bone Matrix
Authors: Medrano-David Daniela , Lopera Aura María , Londoño Martha Elena , Araque-Marín Pedronel
Published: August 2021

The occurrence of bone-related disorders and diseases has dramatically increased in recent years around the world. Demineralized bone matrix (DBM) has been widely used as a bone implant due to its osteoinduction and bioactivity. However, the use of DBM is limited because it is a particulate material, which makes it difficult to manipulate and implant with precision. In addition, these particles are susceptible to migration to other sites. To address this situation, DBM is commonly incorporated into a variety of carriers. An injectable scaffold has advantages over bone grafts or preformed scaffolds, such as the ability to flow and fill a bone defect. The aim of this research was to develop a DBM carrier with such viscoelastic properties in order to obtain an injectable bone substitute (IBS). The developed DBM carrier consisted of a PVA/glycerol network cross-linked with borax and reinforced with CaCO3 as a pH neutralizer, porosity generator, and source of Ca. The physicochemical properties were determined by an injectability test, FTIR, SEM, and TGA. Porosity, degradation, bioactivity, possible cytotoxic effect, and proliferation in osteoblasts were also determined. The results showed that the developed material has great potential to be used in bone tissue regeneration.
Unsupervised Approaches for the Segmentation of Dry ARMD Lesions in Eye Fundus cSLO Images
Authors: Royer Clément , Sublime Jérémie , Rossant Florence , Paques Michel
Published: August 2021

Age-related macular degeneration (ARMD), a major cause of sight impairment for elderly people, is still not well understood despite intensive research. Measuring the size of the lesions in the fundus is the main biomarker of the severity of the disease and as such is widely used in clinical trials yet only relies on manual segmentation. Artificial intelligence, in particular automatic image analysis based on neural networks, has a major role to play in better understanding the disease, by analyzing the intrinsic optical properties of dry ARMD lesions from patient images. In this paper, we propose a comparison of automatic segmentation methods (classical computer vision method, machine learning method and deep learning method) in an unsupervised context applied on cSLO IR images. Among the methods compared, we propose an adaptation of a fully convolutional network, called W-net, as an efficient method for the segmentation of ARMD lesions. Unlike supervised segmentation methods, our algorithm does not require annotated data which are very difficult to obtain in this application. Our method was tested on a dataset of 328 images and has shown to reach higher quality results than other compared unsupervised methods with a F1 score of 0.87, while having a more stable model, even though in some specific cases, texture/edges-based methods can produce relevant results.
Data Augmentation Using Background Replacement for Automated Sorting of Littered Waste
Authors: Patrizi Arianna , Gambosi Giorgio , Zanzotto Fabio Massimo
Published: August 2021

The introduction of sophisticated waste treatment plants is making the process of trash sorting and recycling more and more effective and eco-friendly. Studies on Automated Waste Sorting (AWS) are greatly contributing to making the whole recycling process more efficient. However, a relevant issue, which remains unsolved, is how to deal with the large amount of waste that is littered in the environment instead of being collected properly. In this paper, we introduce BackRep: a method for building waste recognizers that can be used for identifying and sorting littered waste directly where it is found. BackRep consists of a data-augmentation procedure, which expands existing datasets by cropping solid waste in images taken on a uniform (white) background and superimposing it on more realistic backgrounds. For our purpose, realistic backgrounds are those representing places where solid waste is usually littered. To experiment with our data-augmentation procedure, we produced a new dataset in realistic settings. We observed that waste recognizers trained on augmented data actually outperform those trained on existing datasets. Hence, our data-augmentation procedure seems a viable approach to support the development of waste recognizers for urban and wild environments.
Real-Time 3D Multi-Object Detection and Localization Based on Deep Learning for Road and Railway Smart Mobility
Authors: Mauri Antoine , Khemmar Redouane , Decoux Benoit , Haddad Madjid , Boutteau Rémi
Published: August 2021

For smart mobility, autonomous vehicles, and advanced driver-assistance systems (ADASs), perception of the environment is an important task in scene analysis and understanding. Better perception of the environment allows for enhanced decision making, which, in turn, enables very high-precision actions. To this end, we introduce in this work a new real-time deep learning approach for 3D multi-object detection for smart mobility not only on roads, but also on railways. To obtain the 3D bounding boxes of the objects, we modified a proven real-time 2D detector, YOLOv3, to predict 3D object localization, object dimensions, and object orientation. Our method has been evaluated on KITTI’s road dataset as well as on our own hybrid virtual road/rail dataset acquired from the video game Grand Theft Auto (GTA) V. The evaluation of our method on these two datasets shows good accuracy, but more importantly that it can be used in real-time conditions, in road and rail traffic environments. Through our experimental results, we also show the importance of the accuracy of prediction of the regions of interest (RoIs) used in the estimation of 3D bounding box parameters.
Cliff Retreat Contribution to the Littoral Sediment Budget along the Baltic Sea Coastline of Schleswig-Holstein, Germany
Authors: Averes Tanita , Hofstede Jacobus L. A. , Hinrichsen Arfst , Reimers Hans-Christian , Winter Christian
Published: August 2021

Mobile coastal sediments, such as sand and gravel, build up and protect wave-dominated coastlines. In sediment-starved coastal environments, knowledge about the natural sources and transport pathways of those sediments is of utmost importance for the understanding and management of coastlines. Along the Baltic Sea coast of Schleswig-Holstein (Germany), the retreat of active cliffs—made of cohesive Pleistocene deposits—supplies a wide size range of sediments to the coastal system. The material is reworked and sorted by hydrodynamic forcing: the less mobile stones and boulders remain close to the source area; the finest sediments, mostly clay and silt, are transported offshore into areas of low energy; and the fractions of sand and fine gravels mostly remain in the nearshore zone, where they make up the littoral sediment budget. They contribute to the morphodynamic development of sandy coastlines and nearshore bar systems. Exemplarily for this coastal stretch and based on an extensive review of local studies we quantify the volume of the potential littoral sediment budget from cliff retreat. At an average retreat rate of 0.24 m yr−1 (<0.1–0.73 m yr−1), the assessment indicates a weighted average sediment volume of 1.5 m3 yr−1 m−1 (<0.1–9.5 m3 yr−1 m−1) per meter active cliff. For the whole area, this results in an absolute sediment budget Vs,total of 39,000–161,000 m3 yr−1. The accuracy of the results is limited by system understanding and data quality and coverage. The study discusses uncertainties in the calculation of littoral sediment budgets from cliff retreat and provides the first area-wide budget assessment along the sediment-starved Baltic Sea coastline of Schleswig-Holstein.
New Insights into Blood Circulating Lymphocytes in Human Pneumocystis Pneumonia
Authors: Charpentier Eléna , Marques Catherine , Ménard Sandie , Chauvin Pamela , Guemas Emilie , Cottrel Claire , Cassaing Sophie , Fillaux Judith , Valentin Alexis , Blanchard Nicolas , Berry Antoine , Iriart Xavier
Published: August 2021

The host lymphocyte response is decisive in Pneumocystis pneumonia (PCP) pathophysiology but little is known of the specific roles of lymphocyte subpopulations in this fungal infection. Peripheral NK, NKT, B, TCD4+ and TCD8+ subpopulations were compared by immunophenotyping between 20 patients diagnosed with PCP (PCP(+)] and 20 uninfected immunosuppressed patients (PCP(−)). Among PCP(+) subjects, the lymphocyte populations were also compared between surviving and deceased patients. Low B cell count (<40 cells/µL) was more frequent in PCP(+) than in PCP(−) patients (p = 0.03), while there was no difference for the TCD4 count. Among the PCP(+) group, the 7 deceased patients had lower Th1 (p = 0.02) and Tc1 (p = 0.03) populations, higher Th2 response (p = 0.03), higher effector TCD8 (p < 0.01), lower central memory TCD8 (p = 0.04) and reduced NK cells (p = 0.02) compared with the 13 survivors. Th1/Th2 ratio < 17, CD8 Tc1 < 44%, effector TCD8 < 25%, central memory TCD8 < 4%, NK cells < 50 cells/µL and total lymphocytes < 0.75 G/L were associated with a higher risk of mortality (p = 0.003, p = 0.007, p = 0.0007, p = 0.004, p = 0.02 and p = 0.019, respectively). The traditional analysis of TCD4 and TCD8 populations may be insufficient in the context of PCP. It could be completed by using B cells to predict the risk of PCP, and by using lymphocyte subpopulations or total lymphocyte count, which are easy to obtain in all health care facilities, to evaluate PCP prognosis.
Novel Urban Ecosystems: Opportunities from and to Landscape Architecture
Authors: Teixeira Catarina Patoilo , Fernandes Cláudia Oliveira , Ahern Jack
Published: August 2021

Novel assemblages of biotic, abiotic, and social components resulting from human-induced actions (e.g., climate change, land-use change, species movement) have been labeled as “Novel Ecosystems”, or “Novel Urban Ecosystems” when emerging in urban contexts. This concept has been shifting perspectives among some scientists and making them question traditional values about human-nature interactions in a rapidly changing era dominated by anthropogenic actions (Anthropocene). Controversial dimensions surrounding the Novel Ecosystems and Novel Urban Ecosystems terms may be preventing the evolution and further research of these concepts. The environmental problems that our society will soon face support a search for innovative solutions and transdisciplinary efforts. For that reason, this discussion should not cease, rather should expand to other fields of knowledge that can contribute with pertinent insights and collaborations. This way, this short communication aims to reflect on the opportunities from Landscape Architecture to the discussion, research, and application of the novel ecosystems concepts in the real world, particularly in the urban landscape, and also reflect on the opportunities of this debate to the Landscape Architecture field. Ultimately, Landscape Architecture can contribute with innovative and creative perspectives, acceding valuable and advanced tools, facilitating dialogues between fields of knowledge, and bridging gaps between science, people, and nature.
Tungsten Disulfide Inorganic Nanotubes Functionalized by PTFE for Friction Application
Authors: Levin Tzuriel , Harel Yifat , Lellouche Jean-Paul , Moshkovich Alexey , Lapsker Igor , Laikhtman Alex , Rapoport Lev
Published: August 2021

Inorganic nanotubes (INTs) and fullerene-like nanoparticles (NPs) of WS2/MoS2 penetrate and exfoliate at the contact interface and facilitate tribofilm formation. While the tribological properties are greatly improved by exfoliated NPs that shed easily, they may be diminished by agglomeration in oil. Therefore, surface functionalization is employed to improve dispersion in oil-based suspensions. Here, WS2 INTs were functionalized by polytetrafluoroethylene (PTFE) in a simple and cost-effective bath sonication method. WS2-INTs with two concentrations of added PTFE were characterized by scanning and transmission electron microscopy, micro-Raman spectroscopy, and thermogravimetric analysis. Superior distribution of WS2 was observed before and during friction experiments. Chemical analysis showed a significantly greater amount of PTFE-coated INTs on rubbed surfaces, in accordance with the improved friction and wear properties.
Statistical Machine Learning in Model Predictive Control of Nonlinear Processes
Authors: Wu Zhe , Rincon David , Gu Quanquan , Christofides Panagiotis D.
Published: August 2021

Recurrent neural networks (RNNs) have been widely used to model nonlinear dynamic systems using time-series data. While the training error of neural networks can be rendered sufficiently small in many cases, there is a lack of a general framework to guide construction and determine the generalization accuracy of RNN models to be used in model predictive control systems. In this work, we employ statistical machine learning theory to develop a methodological framework of generalization error bounds for RNNs. The RNN models are then utilized to predict state evolution in model predictive controllers (MPC), under which closed-loop stability is established in a probabilistic manner. A nonlinear chemical process example is used to investigate the impact of training sample size, RNN depth, width, and input time length on the generalization error, along with the analyses of probabilistic closed-loop stability through the closed-loop simulations under Lyapunov-based MPC.