bluuebunny/crossref_metadata_2025 huggingface dataset:
Relationships Between Schistosoma mansoni Infection Intensity and Nutritional Status and Anemia Among Preschool-aged Children in Uganda
Authors: Colt Susannah , Miller Cole D , Edielu Andrew , Webb Emily L , Mawa Patrice A , Wu Hannah W , Nakyesige Racheal , Muheki Edridah , Kabatereine Narcis , Bustinduy Amaya L , Friedman Jennifer F
Published: August 2023

In a cross-sectional analysis of 354 Ugandan children (age 12–48 months) infected with Schistosoma mansoni, we assessed relationships between infection intensity and nutritional morbidities. Higher intensity was associated with an increased risk for anemia (RR = 1.05, 95% confidence interval [CI] 1.01–1.10) yet not associated with risk for underweight, stunting, or wasting.
Identifying Effective Durations of Antibiotic Therapy for the Treatment of Carbapenem-resistant Enterobacterales Bloodstream Infections: A Multicenter Observational Study
Authors: Soto Caitlin L , Hsu Alice J , Lee Jae Hyoung , Dzintars Kathryn , Choudhury Rebecca , Jenkins Timothy C , McCreary Erin K , Quartuccio Katelyn S , Stohs Erica J , Zimmerman Matty , Tamma Pranita D
Published: August 2023

In a propensity-score-weighted cohort of 183 adults with carbapenem-resistant Enterobacterales bacteremia at 24 US hospitals, patients receiving short courses of active therapy (7–10 days, median 9 days) experienced similar odds of recurrent bacteremia or death within 30 days as those receiving prolonged courses of active therapy (14–21 days, median 14 days).
Coping with drought? The hidden microhabitat selection and underground movements of amphisbaenians under summer drought conditions
Authors: Martín José , Rodríguez-Ruiz Gonzalo , Cuervo José Javier
Published: August 2023

Habitat selection is a dynamic process that depends on many environmental variables that can vary with weather conditions. This is important because, within a context of global change, extreme weather events, such as severe droughts, are predicted to become more frequent. We examined the patterns of microhabitat selection and underground movements (using PIT-tag telemetry) of a strictly fossorial reptile, the North African checkboard amphisbaenian Trogonophis wiegmanni, during the summer drought period. We aimed to test whether changes in strategies of habitat use and movements could allow this amphisbaenian to cope with unfavorable weather. We found that during the summer drought period, T. wiegmanni did not use the microhabitats in relationship to their availability, but particularly selected sites with high abundance of rocks but also areas under a high cover of bushes, where environmental conditions were more favorable. We also found, using PIT-tag telemetry, that the numbers of T. wiegmanni individuals located under rocks and their activity (number of days with movements) decreased largely in summer. However, the animals were not entirely inactive, but, especially males were active below the ground under bushes and made some relatively long underground hidden movements between favorable areas.
Race and Ethnic Representation in Crohn’s Disease Trials of Biologic and Small Molecule Medications: A Systematic Review and Meta-analysis
Authors: Pelton Matt , Ssentongo Paddy , Sun Ashley , Groff Destin , Dalessio Shannon , Clarke Kofi
Published: August 2023

Background and Aims

Randomised controlled trials historically under-represent marginalised racial and ethnic populations. As incidence and prevalence of Crohn’s disease in these groups rise, it is important to characterise their inclusion in randomised controlled trials on first-line and pipe-line medications.

Methods

PubMed was searched systematically for randomised controlled trials of biologic and small molecule inhibitor [SMI] medications, with a primary outcome related to efficacy following PRISMA guidelines. We used descriptive statistics to summarise demographic variables and meta-regression analyses to estimate temporal trends in racial inclusion.

Results

More than a half of trials did not report any racial/ethnic demographics [53.7%] and several reported racial demographics for only one race [20.9%]. When racial data were reported, Whites made up 90.2% of participants. Percentages of Black, Asian, Native American/Pacific Islander, and participants considered ‘Other’ averaged 2.9%, 11.6%, 0.5%, and 1.6% out of the total sample sizes of 3901, 3742, 828 and 4027, respectively. Proportional representation of White participants decreased over time [p <0.01] and proportional representation of Asian participants increased over time [p = 0.047]. In ordinal logistic regression, mean year of trial enrolment significantly increased the number of racial groups reported [p <0.001].

Conclusions

Half of published randomised controlled trials in Crohn’s disease contain no racial or ethnic demographics, and the remaining often only have limited inclusion of Black, Native American/Pacific Islander, and Hispanic patients. Further work should characterise representation in observational and prospective trials. Researchers should work to: 1] increase reporting of racial and ethnic demographics; and 2] improve recruitment and retention of marginalised populations.
Determinants and prognostic implications of left atrial reverse remodelling after coarctation of aorta repair in adults
Authors: Egbe Alexander C , Younis Ahmed , Miranda William R , Jain C Charles , Connolly Heidi M , Borlaug Barry A
Published: August 2023

Aims

Left atrial (LA) dysfunction and atrial fibrillation are also relatively common in adults with coarctation of aorta (COA), and the severity of LA dysfunction is associated with a higher risk of atrial fibrillation in this population. The purpose of this study was to determine whether LA function improved after COA repair (LA reverse remodelling), and the relationship between LA reverse remodelling and atrial fibrillation.

Methods and results

Retrospective cohort study of adults undergoing COA repair (2003–20). LA reservoir strain was assessed pre intervention and 12–24 months post intervention, using speckle tracking echocardiography. Incident atrial fibrillation was assessed from COA repair to last follow-up. Of 261 adults who underwent COA repair [age 37 ± 13 years; males 148 (57%)], 124 (47%) and 137 (53%) presented with native vs. recurrent COA, respectively. Of 261 patients, 231 (82%) and 48 (18%) underwent surgical and transcatheter COA repair, respectively. The LA reservoir strain increased from 32 ± 8% (pre intervention) to 39 ± 7% (post intervention), yielding a relative increase of 21 ± 5%. Older age [β ± standard error (SE) −0.16 ± 0.09 per 5 years, P = 0.02], higher systolic blood pressure (β ± SE −0.12 ± 0.04 per 5 mmHg, P = 0.005), and higher residual COA mean gradient (β ± SE −0.17 ± 0.06 per 5 mmHg, P = 0.002) post intervention were associated with less LA reverse remodelling, after adjustment for sex, hypertension diagnosis, and left ventricular indices. LA reverse remodelling (hazard ratio 0.97, 95% confidence interval 0.96–0.98 per 1% increase from pre-intervention LA function, P = 0.006) was associated with a lower risk of atrial fibrillation after adjustment for age, sex, pre-intervention LA reservoir strain, and history of atrial fibrillation.

Conclusion

COA repair resulted in improved LA function and decreased risk for atrial fibrillation, especially in patients without residual hypertension or significant residual COA gradient.
A case of ultra-prolonged intra-aortic balloon pump support via sheathless femoral access
Authors: Al-Ani Mohammad A , Snipes Garrett , Parker Alex M , Kerensky Richard A
Published: August 2023

Background

An intra-aortic balloon pump (IABP) is a mechanical circulatory support platform with a relatively low complication rate. Axillary access is increasingly utilized to allow rehabilitation.

Case summary

We present a case of femoral IABP inserted into the femoral artery percutaneously via a sheathless technique that allowed the patient to ambulate and physically rehabilitate over 102 days until cardiac transplantation. The patient was able to progress with the protocolized rehabilitation programme to up to 3500 ft walking distance. The IABP was removed at the time of transplantation without any vascular complications.

Discussion

While axillary IABP offers an opportunity to rehabilitate, it has an unacceptably high complication rate, often resulting in vascular injury that adds morbidity to an acutely ill cohort. In this case, we found that sheathless femoral IABP access offered stability for a prolonged time while avoiding pain, bleeding, infection, and vascular injury. We hypothesize that this is due to less indwelling prosthetic material usage and also device flexibility, allowing conformation to the natural course of the femoral artery. We are encouraged by this case to use a sheathless access approach for patients expected to require prolonged IABP support.
Is the Epithor conversion score reliable in robotic-assisted surgery anatomical lung resection?
Authors: Armand Elsa , Fourdrain Alex , Lafouasse Chloé , Resseguier Noémie , Trousse Delphine , D’Journo Xavier-Benoît , Thomas Pascal-Alexandre
Published: August 2023

OBJECTIVES

Despite an improvement in surgical abilities, the need for an intraoperative switch from a minimally invasive procedure towards an open surgery (conversion) still remains. To anticipate this risk, the Epithor conversion score (ECS) has been described for video-assisted thoracoscopic surgery (VATS). Our objective was to determine if this score, developed for VATS, is applicable in robotic-assisted thoracoscopic surgery (RATS).

METHODS

This was a retrospective monocentric study from January 2006 to June 2022, and data were obtained from the EPITHOR database. Patients included were those who underwent anatomic lung resection either by VATS or RATS. The ECS was calculated for all patients studied. Discrimination and calibration of the test were measured by the area under the curve and Hosmer–Lemeshow test.

RESULTS

A total of 1685 were included. There were 183/1299 conversions in the VATS group (14.1%) and 27/386 conversions in the RATS group (6.9%). Patients in the RATS group had fewer antiplatelet therapy and peripheral arterial disease. There were more segmentectomies in the VATS group. As for test discrimination, the area under the curve was 0.66 [0.56–0.78] in the RATS group and 0.64 [0.60–0.69] in the VATS group. Regarding the calibration, the Hosmer–Lemeshow test was not significant for both groups but more positive (better calibrated) for the VATS group (P = 0.12) compared to the RATS group (P = 0.08).

CONCLUSIONS

The ECS seems applicable for patients operated with RATS, with a correct discrimination but a lower calibration performance for patients operated with VATS. A new score could be developed to specifically anticipate conversion in patients operated on by RATS.
Gut bacterial communities in Atlantic bottlenose dolphins (Tursiops truncatus) throughout a disease-driven (Morbillivirus) unusual mortality event
Authors: Olmstead Alyssa R B , Mathieson Olivia L , McLellan William A , Pabst D Ann , Keenan Tiffany F , Goldstein Tracey , Erwin Patrick M
Published: August 2023

Gut microbiomes are important determinants of animal health. In sentinel marine mammals where animal and ocean health are connected, microbiome impacts can scale to ecosystem-level importance. Mass mortality events affect cetacean populations worldwide, yet little is known about the contributory role of their gut bacterial communities to disease susceptibility and progression. Here, we characterized bacterial communities from fecal samples of common bottlenose dolphins, Tursiops truncatus, across an unusual mortality event (UME) caused by dolphin Morbillivirus (DMV). 16S rRNA gene sequence analysis revealed similar diversity and structure of bacterial communities in individuals stranding before, during, and after the 2013–2015 Mid-Atlantic Bottlenose Dolphin UME and these trends held in a subset of dolphins tested by PCR for DMV infection. Fine-scale shifts related to the UME were not common (10 of 968 bacterial taxa) though potential biomarkers for health monitoring were identified within the complex bacterial communities. Accordingly, acute DMV infection was not associated with a distinct gut bacterial community signature in T. truncatus. However, temporal stratification of DMV-positive dolphins did reveal changes in bacterial community composition between early and late outbreak periods, suggesting that gut community disruptions may be amplified by the indirect effects of accumulating health burdens associated with chronic morbidity.
Developmental Diet Alters the Fecundity–Longevity Relationship and Age-Related Gene Expression in Drosophila melanogaster
Authors: Collins David H , Prince David C , Donelan Jenny L , Chapman Tracey , Bourke Andrew F G
Published: August 2023

The standard evolutionary theory of aging predicts a negative relationship (trade-off) between fecundity and longevity. However, in principle, the fecundity–longevity relationship can become positive in populations in which individuals have unequal resources. Positive fecundity–longevity relationships also occur in queens of eusocial insects such as ants and bees. Developmental diet is likely to be central to determining trade-offs as it affects key fitness traits, but its exact role remains uncertain. For example, in Drosophila melanogaster, changes in adult diet can affect fecundity, longevity, and gene expression throughout life, but it is unknown how changes in developmental (larval) diet affect fecundity–longevity relationships and gene expression in adults. Using D. melanogaster, we tested the hypothesis that varying developmental diets alters the directionality of fecundity–longevity relationships in adults, and characterized associated gene expression changes. We reared larvae on low (20%), medium (100%), and high (120%) yeast diets, and transferred adult females to a common diet. We measured fecundity and longevity of individual adult females and profiled gene expression changes with age. Adult females raised on different larval diets exhibited fecundity–longevity relationships that varied from significantly positive to significantly negative, despite minimal differences in mean lifetime fertility or longevity. Treatments also differed in age-related gene expression, including for aging-related genes. Hence, the sign of fecundity–longevity relationships in adult insects can be altered and even reversed by changes in larval diet quality. By extension, larval diet differences may represent a key mechanistic factor underpinning positive fecundity–longevity relationships observed in species such as eusocial insects.
Heterogeneity in Measures and Rates of Reported Dementia and Subjective Memory Complaints Across U.S. National Surveys
Authors: Picchiello Matthew C , Carpenter Brian D
Published: August 2023

Objectives

Several U.S. health surveillance surveys contain items related to self- and proxy reports of dementia and subjective memory complaints (SMC). Despite their similar content, these items differ in terminology, item specificity, and time frame. The goal of this study was to analyze whether item features might influence endorsement rates for dementia and SMC.

Methods

We calculated design-appropriate estimates for the endorsement of dementia and SMC across U.S.-based national surveys and employed pairwise comparisons to evaluate endorsement rates across surveys. We also examined item characteristics to explore possible effects on endorsement rates.

Results

Endorsement rates were wide-ranging for dementia (ranging from 2.7% to 9.9%) and SMC (5.6% to 46.6%). Pairwise comparisons revealed statistically significant differences on most dementia-related items (76%), and all SMC comparisons (100%). Items varied substantially in the terminology used to assess dementia and SMC (e.g., “dementia” vs “Alzheimer’s disease”) and used different time frames (e.g., “past month” vs “5 years”).

Discussion

National survey data on reported dementia and SMC can have important research, training, and policy implications, yet endorsement rates vary widely across surveys. That variability could emerge from subtle but influential item characteristics, and our findings highlight the need for item harmonization, in even their most basic characteristics. Standardizing items across national surveillance surveys facilitates comparison across surveys so that we can better understand the true burden of these conditions to inform public health initiatives.
Does Age Matter? Tweets About Gerontocracy in the United States
Authors: Ng Reuben , Indran Nicole
Published: August 2023

Objectives

Discussions on the political scene in the United States have been accompanied by the notion that the country has morphed into a gerontocracy, a system of governance where power is wielded predominantly by older individuals. With rising life expectancy, more as well as older candidates are likely to run for and stay in office. This study analyzes perceptions regarding the older political leadership of the United States.

Methods

We collected 3,599 tweets containing the word “gerontocracy” which were uploaded between September 10, 2022 and November 10, 2022, a month prior to the midterm elections in the United States, when this topic was likely to have sparked considerable discussion. After applying our exclusion criteria, 1,266 tweets were retained. Our qualitative content analysis was performed inductively and deductively.

Results

Seven themes emerged. Most tweets (24%; N = 304) highlighted that “Gerontocracy is a Problem” (Theme 1). Theme 2 (21%) focused on how “Democracy is a Sham” (N = 270). Theme 3 (19%) positioned “Old Age as the Problem and Youth as the Solution” (N = 234). Theme 4 (11%) was about how “The Future is at Stake” (N = 145). “Health Concerns” dominated Theme 5 (10%; N = 126). Theme 6 looked at “Structural Reforms” (9%, N = 115). Theme 7 “In Defense of the Gerontocracy” formed 6% of the data set (N = 72).

Discussion

Attitudes toward older politicians are commonly tied to negative stereotypes surrounding old age. As the population ages, it is paramount that society focuses on potentially more important considerations in the evaluation of the actual caliber of a politician.
A single-step, rapid, and versatile method for simultaneous detection of cell surface glycan profiles using fluorochrome-conjugated lectins
Authors: Torres Nicolás I , Manselle Cocco Montana N , Perrotta Ramiro M , Mahmoud Yamil D , Salatino Mariana , Mariño Karina V , Rabinovich Gabriel A
Published: August 2023

Cell surface glycans play essential roles in diverse physiological and pathological processes and their assessment has important implications in biomedicine and biotechnology. Here we present a rapid, versatile, and single-step multicolor flow cytometry method for evaluation of cell surface glycan signatures using a panel of selected fluorochrome-conjugated lectins. This procedure allows simultaneous detection of cell surface glycans with a 10-fold reduction in the number of cells required compared with traditional multistep lectin staining methods. Interestingly, we used this one-step lectin array coupled with dimension reduction algorithms in a proof-of-concept application for discrimination among different tumor and immune cell populations. Moreover, this procedure was also able to unveil T-, B-, and myeloid cell subclusters exhibiting differential glycophenotypes. Thus, we report a rapid and versatile lectin cytometry method to simultaneously detect a particular repertoire of surface glycans on living cells that can be easily implemented in different laboratories and core facilities.
Mutations in human DNA methyltransferase DNMT1 induce specific genome-wide epigenomic and transcriptomic changes in neurodevelopment
Authors: Davis Kasey N , Qu Ping-Ping , Ma Shining , Lin Ling , Plastini Melanie , Dahl Niklas , Plazzi Giuseppe , Pizza Fabio , O’Hara Ruth , Wong Wing Hung , Hallmayer Joachim , Mignot Emmanuel , Zhang Xianglong , Urban Alexander E
Published: August 2023

DNA methyltransferase type 1 (DNMT1) is a major enzyme involved in maintaining the methylation pattern after DNA replication. Mutations in DNMT1 have been associated with autosomal dominant cerebellar ataxia, deafness and narcolepsy (ADCA-DN). We used fibroblasts, induced pluripotent stem cells (iPSCs) and induced neurons (iNs) generated from patients with ADCA-DN and controls, to explore the epigenomic and transcriptomic effects of mutations in DNMT1. We show cell type–specific changes in gene expression and DNA methylation patterns. DNA methylation and gene expression changes were negatively correlated in iPSCs and iNs. In addition, we identified a group of genes associated with clinical phenotypes of ADCA-DN, including PDGFB and PRDM8 for cerebellar ataxia, psychosis and dementia and NR2F1 for deafness and optic atrophy. Furthermore, ZFP57, which is required to maintain gene imprinting through DNA methylation during early development, was hypomethylated in promoters and exhibited upregulated expression in patients with ADCA-DN in both iPSC and iNs. Our results provide insight into the functions of DNMT1 and the molecular changes associated with ADCA-DN, with potential implications for genes associated with related phenotypes.
Sex and Biology: Broader Impacts Beyond the Binary
Authors: Sharpe Sam L , Anderson Andrew P , Cooper Idelle , James Timothy Y , Kralick Alexandra E , Lindahl Hans , Lipshutz Sara E , McLaughlin J F , Subramaniam Banu , Weigel Alicia Roth , Lewis A Kelsey
Published: August 2023

Synopsis

What are the implications of misunderstanding sex as a binary, and why is it essential for scientists to incorporate a more expansive view of biological sex in our teaching and research? This roundtable will include many of our symposium speakers, including biologists and intersex advocates, to discuss these topics and visibilize the link between ongoing reification of dyadic sex within scientific communities and the social, political, and medical oppression faced by queer, transgender, and especially intersex communities. As with the symposium as a whole, this conversation is designed to bring together empirical research and implementation of equity, inclusion, and justice principles, which are often siloed into separate rooms and conversations at academic conferences. Given the local and national attacks on the rights of intersex individuals and access to medical care and bodily autonomy, this interdisciplinary discussion is both timely and urgent.
Changes in Inflammatory Cytokine Levels in Rectal Mucosa Associated With Neisseria gonorrheae and/or Chlamydia trachomatis Infection and Treatment Among Men Who Have Sex With Men in Lima, Peru
Authors: Clark Jesse L , Oldenburg Catherine E , Passaro Ryan C , Segura Eddy R , Godwin William , Fulcher Jennifer A , Cabello Robinson
Published: August 2023

Background

Neisseria gonorrheae and Chlamydia trachomatis are associated with mucosal inflammation and human immunodeficiency virus 1 (HIV-1) transmission. We assessed levels of inflammatory cytokines in men who have sex with men (MSM) with and without rectal gonorrhea and/or chlamydia in Lima, Peru.

Methods

We screened 605 MSM reporting condomless receptive anal intercourse for rectal N. gonorrheae/C. trachomatis using nucleic acid testing. We identified 101 cases of gonorrhea and/or chlamydia and randomly selected 50 N. gonorrheae/C. trachomatis positive cases and matched 52 negative controls. We measured levels of IL-1β, IL-6, IL-8, and TNF-α in rectal secretions. Tests for HIV-1, rectal N. gonorrheae/C. trachomatis, and mucosal cytokines were repeated after 3 and 6 months. Cytokine levels in cases and uninfected controls were compared using Wilcoxon rank-sum tests and linear regression.

Results

MSM with gonorrhea/chlamydia had elevated levels of all cytokines in rectal mucosa compared with matched controls (all P values <.001). Following antibiotic treatment there were no significant differences in cytokine levels at 3- or 6-month follow-up evaluations (all P values >.05).

Discussion

Rectal gonorrhea/chlamydia infection is associated with transient mucosal inflammation and cytokine recruitment. Our data provide proof of concept for rectal sexually transmitted infection screening as an HIV prevention strategy for MSM.

Clinical Trials Registration. NCT03010020.
GoodFibes: An R Package for The Detection of Muscle Fibers from diceCT Scans
Authors: Arbour J H
Published: August 2023

Synopsis

Contrast enhanced computed-tomography imaging like diffusible iodine-based contrast-enhanced computed tomography (diceCT) can provide detailed information on muscle architecture important to comparative analyses of functional morphology, using non-destructive approaches. However, manual segmentation of muscle fascicles/fibers is time-consuming, and automated approaches are at times inaccessible and unaffordable. Here, we introduce GoodFibes, an R package for reconstructing muscle architecture in 3D from diceCT image stacks. GoodFibes uses textural analysis of image grayscale values to track straight or curved fiber paths through a muscle image stack. Accessory functions provide quality checking, fiber merging, and 3D visualization and export capabilities. We demonstrate the utility and effectiveness of GoodFibes using two datasets, from an ant and bat diceCT scans. In both cases, GoodFibes provides reliable measurements of mean fiber length compared to traditional approaches, and is as effective as currently available software packages. This open-source, free to use software package will help to improve access to tools in the analysis of muscle fiber anatomy using diceCT scans. The flexible and transparent R-language environment allows other users to build on the functions described here and permits direct statistical analysis of the resulting fiber metrics. We hope that this will increase the number of comparative and evolutionary studies incorporating these rich and functionally important datasets.
Use of Real Time Clinical Video Telehealth to Home by primary care providers within the Veterans Health Administration during the first wave of the COVID-19 pandemic: variability across VA stations and with time
Authors: Sullivan Dennis H , Sawyer Linda M , Dawson Bonnie D , Dunlap Janette , Cigolle Christine T , Keller Hallie E , Burningham Zachary
Published: August 2023

Objective

Determine the extent to which use of Clinical Video Telehealth to Home (VT2H) for primary care licensed independent practitioner visits (PCLIPVs) varied over time and across the Veterans Health Administration (VA) during the first 18 months of the COVID pandemic, and if there was an association between VT2H usage and VA station characteristics.

Materials and Methods

All outpatient encounters (n = 12 143 456) for Veterans (n = 4 373 638) that had VA PCLIPVs during the period of observation were categorized as conducted by VT2H, in-person, or telephone. The change over time in the percentage of total PCLIPVs conducted by VT2H was plotted and associations between VA station characteristics and VT2H usage were analyzed using simple statistics and negative binomial regression.

Results

Between March 2020 and mid-August 2020, VT2H visits increased from <2% to 13% of all VA PCLIPVs. However, VT2H usage varied substantively by VA station and declined system-wide to <9% of PCLIPVs by July 2021. VA stations that serve a greater proportion of rural Veterans were found less likely to use VT2H.

Discussion

The VA was successful in increasing the use of VT2H for PCLIPVs during the first phase of the COVID pandemic. However, VT2H usage varied by VA station and over time. Beyond rurality, it is unknown what station characteristics may be responsible for the variance in VT2H use.

Conclusion

Future investigation is warranted to identify the unique practices employed by VA stations that were most successful in using VT2H for PCLIPVs and whether they can be effectively disseminated to other stations.
A national evaluation of adjuvant chemotherapy in pT4N0M0 colon cancer from the National Cancer Database
Authors: Reif de Paula Thais , Keller Deborah S
Published: August 2023

Background

T stage is a prognostic biomarker for overall survival in colon cancer and pathologic T4 disease is a high-risk characteristic. Adjuvant chemotherapy is recommended to improve overall survival in pT4N0M0, but compliance with guidelines is unknown. We aimed to evaluate adjuvant chemotherapy use and impact on overall survival in pT4N0M0 colon cancer.

Methods

The National Cancer Database was reviewed for pT4N0M0 colon adenocarcinomas undergoing curative surgical resection (2010-2017). Cases were stratified into no adjuvant chemotherapy and adjuvant chemotherapy cohorts. Moderated multiple regression assessed factors associated with no AC. Kaplan–Meier and Cox regression assessed overall survival in propensity-score matched cohorts. The main outcome measures were adjuvant chemotherapy use, factors associated with adjuvant chemotherapy, and impact on overall survival.

Results

Of 11 847 cases, 62.4% (n = 7391) received no adjuvant chemotherapy. With private insurance, comorbidities or income do not affect adjuvant chemotherapy use. Medicare cases with a Charlson–Deyo comorbidity index of 0 (odds ratio [OR] = 0.861, 95% confidence interval [CI] = 0.760 to 0.975; P = .019) and Medicare payors with high income (OR = 0.813, 95% CI = 0.690 to 0.959; P = .014) were associated with adjuvant chemotherapy. Medicaid Charlson–Deyo comorbidity index 0 (OR = 1.374, 95% CI = 1.125 to 1.679; P = .002) and uninsured Charlson–Deyo comorbidity index 0 (OR = 1.351, 95% CI = 1.120 to 1.629; P = .002) were associated with no adjuvant chemotherapy. Adjuvant chemotherapy was associated with improved 5-year overall survival (71.7% vs 56.4%; P < .001; adjusted hazard ratio = 0.543, 95% CI = 0.499 to 0.590; P < .001).

Conclusion

Although adjuvant chemotherapy is associated with improved overall survival, compliance is low. There is a complex relationship between payor, income, comorbidity, and adjuvant chemotherapy receipt. Medicare patients with no comorbidities or higher income have better adjuvant chemotherapy use. With private insurance, adjuvant chemotherapy compliance is not affected by comorbidities or income, whereas Medicaid and uninsured patients with no comorbidities have poor compliance. Future work could target these disparities for equitable care.
Legumain: a potential biomarker for atherosclerosis
Authors: Ke Bo-yi , Qin Yu-sheng , Cheng Lu , Wang Shu-zhi
Published: August 2023

Atherosclerosis is a lipid-driven chronic inflammatory disease that poses a serious threat to health. Legumain (LGMN), also known as asparagine endonuclease, is a new type of cysteine proteases that can specifically hydrolyze substrate molecules containing asparagine residues. It has anti-apoptotic effects in mammals and plays an antigen-presenting role in inflammatory response. Several studies have found that LGMN can activate multiple signal pathways to promote cell apoptosis and migration, inflammatory response, and the development of atherosclerosis. Importantly, LGMN exerts pro-atherogenic effects by participating in a variety of pathophysiological mechanisms of atherosclerosis, including vascular remodeling, inflammatory response, plaque stability, and the degradation of extracellular matrix. In the present review, we describe the LGMN distribution, structure, generation, and functional partners. Furthermore, we summarize the relationship between LGMN and atherosclerosis. Based on the relationship between LGMN and atherosclerosis, LGMN may be a potential biomarker for atherosclerosis.
Deep learning-based high-throughput detection of in vitro germination to assess pollen viability from microscopic images
Authors: Zhang Mengwei , Zhao Jianxiang , Hoshino Yoichiro
Published: August 2023

In vitro pollen germination is considered the most efficient method to assess pollen viability. The pollen germination frequency and pollen tube length, which are key indicators of pollen viability, should be accurately measured during in vitro culture. In this study, a Mask R-CNN model trained using microscopic images of tree peony (Paeonia suffruticosa) pollen has been proposed to rapidly detect the pollen germination rate and pollen tube length. To reduce the workload during image acquisition, images of synthesized crossed pollen tubes were added to the training dataset, significantly improving the model accuracy in recognizing crossed pollen tubes. At an Intersection over Union threshold of 50%, a mean average precision of 0.949 was achieved. The performance of the model was verified using 120 testing images. The R2 value of the linear regression model using detected pollen germination frequency against the ground truth was 0.909 and that using average pollen tube length was 0.958. Further, the model was successfully applied to two other plant species, indicating a good generalizability and potential to be applied widely.
Calbindin Has a Potential Spatiotemporal Correlation with Proliferation and Apoptosis in the Postnatal Rat Kidney
Authors: Mahmoud Sahar F , Elewa Yaser H , Nomir Ahmed G , Rashwan Ahmed M , Noreldin Ahmed E
Published: August 2023

The protein calbindin-D28k modulates calcium reabsorption in the kidney. Here, we aimed to study the influence of proliferation and apoptosis in different compartments of the kidney on the developmental function of calbindin. Using immunohistochemistry, we investigated the postnatal development of rats’ kidneys by using calbindin, proliferative cell nuclear antigen (PCNA), and apoptotic single-stranded DNA (ssDNA). In the neonatal stage (1-day and 1-week-old rats), calbindin showed a positive reaction in the distal convoluted tubule (DCT), a short nephron segment between the macula densa, collecting ducts, and tubules. Moreover, the localization of calbindin was restricted to immature nephrons and mesenchymal tissues. Furthermore, PCNA immunoreactivity was moderate in early-developed podocytes with no reactivity in other renal tubules. The ssDNA immunoreactivity was moderate in the undifferentiated nephron. Then, in the mature stage (3 and 6 weeks old), there was an intense calbindin reaction in DCT but a moderate reaction to PCNA and ssDNA in podocytes. A more intense calbindin reactivity was found in the adult stage (2- and 3-month-old rats) in DCT and collecting tubules. Therefore, in this study, calbindin localization showed an inverse relationship with PCNA and ssDNA of the nephron compartments, which might reflect the efficiency of bone-building and muscle contraction during animal development.
3D X-ray Histology for the Investigation of Temporal Lobe Epilepsy in a Mouse Model
Authors: Rodgers Griffin , Bikis Christos , Janz Philipp , Tanner Christine , Schulz Georg , Thalmann Peter , Haas Carola A , Müller Bert
Published: August 2023

The most common form of epilepsy among adults is mesial temporal lobe epilepsy (mTLE), with seizures often originating in the hippocampus due to abnormal electrical activity. The gold standard for the histopathological analysis of mTLE is histology, which is a two-dimensional technique. To fill this gap, we propose complementary three-dimensional (3D) X-ray histology. Herein, we used synchrotron radiation-based phase-contrast microtomography with 1.6 μm-wide voxels for the post mortem visualization of tissue microstructure in an intrahippocampal-kainate mouse model for mTLE. We demonstrated that the 3D X-ray histology of unstained, unsectioned, paraffin-embedded brain hemispheres can identify hippocampal sclerosis through the loss of pyramidal neurons in the first and third regions of the Cornu ammonis as well as granule cell dispersion within the dentate gyrus. Morphology and density changes during epileptogenesis were quantified by segmentations from a deep convolutional neural network. Compared to control mice, the total dentate gyrus volume doubled and the granular layer volume quadrupled 21 days after injecting kainate. Subsequent sectioning of the same mouse brains allowed for benchmarking 3D X-ray histology against well-established histochemical and immunofluorescence stainings. Thus, 3D X-ray histology is a complementary neuroimaging tool to unlock the third dimension for the cellular-resolution histopathological analysis of mTLE.
Hunting for the candidates of misclassified sources in LSP BL Lacs using machine learning
Authors: Kang Shi-Ju , Zheng Yong-Gang , Wu Qingwen
Published: August 2023

An equivalent–width-based classification may cause the erroneous judgement to the flat spectrum radio quasars (FSRQs) and BL Lacerate objects (BL Lac) due to the diluting the line features by dramatic variations in the jet continuum flux. To help address the issue, this work explores the possible intrinsic classification on the basis of a random forest supervised machine learning algorithm. In order to do so, we compile a sample of 1680 Fermi blazars that have both gamma-rays and radio-frequencies data available from the 4LAC-DR2 catalogue, which includes 1352 training and validation samples and 328 forecast samples. By studying the results for all of the different combinations of 23 characteristic parameters, we found that there are 178 optimal parameter combinations (OPCs) with the highest accuracy (≃98.89 per cent). Using the combined classification results from the nine combinations of these OPCs to the 328 forecast samples, we predict that there are 113 true BL Lacs (TBLs) and 157 false BL Lacs (FBLs) that are possible intrinsically FSRQs misclassified as BL Lacs. The FBLs show a clear separation from TBLs and FSRQs in the gamma-ray photon spectral index, Γph, and X-band radio flux, logFR, plot. Phenomenally, existence a BL Lac to FSRQ (B-to-F) transition zone is suggested, where the FBLs are in the stage of transition from BL Lacs to FSRQs. Comparing the LSP changing-look blazars (CLBs) reported in the literatures, the majority of LSP CLBs are located at the B-to-F zone. We argue that the FBLs located at B-to-F transition zone are the most likely candidates of CLBs.
CO-CHANGES – I. IRAM 30-m CO observations of molecular gas in the sombrero galaxy
Authors: Jiang Yan , Li Jiang-Tao , Gao Yu , Bregman Joel N , Ji Li , Jiang Xue-Jian , Tan Qing-Hua , Wang Jian-Fa , Wang Q Daniel , Yang Yang
Published: August 2023

Molecular gas plays a critical role in explaining the quiescence of star formation (SF) in massive isolated spiral galaxies, which could be a result of either the low molecular gas content and/or the low SF efficiency. We present IRAM 30-m observations of the CO lines in the Sombrero galaxy (NGC 4594), the most massive spiral at $d\lesssim 30\rm ~Mpc$. We detect at least one of the three CO lines covered by our observations in all 13 observed positions located at the galactic nucleus and along an $\sim 25\rm ~kpc$-diameter dusty ring. The total extrapolated molecular gas mass of the galaxy is $M_{\rm H_2}\approx 4\times 10^{8}\rm ~M_\odot$. The measured maximum CO gas rotation velocity of $\approx 381\rm ~km~s^{-1}$ suggests that NGC 4594 locates in a dark matter halo with a mass $M_{\rm 200}\gtrsim 10^{13}\rm ~M_\odot$. Comparing to other galaxy samples, NGC 4594 is extremely gas poor and SF inactive, but the SF efficiency is apparently not inconsistent with that predicted by the Kennicutt–Schmidt law, so there is no evidence of enhanced SF quenching in this extremely massive spiral with a huge bulge. We also calculate the predicted gas supply rate from various sources to replenish the cold gas consumed in SF, and find that the galaxy must experience a starburst stage at high redshift, then the leftover or recycled gas provides SF fuels to maintain the gradual growth of the galactic disc at a gentle rate.
Inhibition of Wnt activity improves peri-implantation development of somatic cell nuclear transfer embryos
Authors: Li Yanhe , Zheng Caihong , Liu Yingdong , He Jincan , Zhang Qiang , Zhang Yalin , Kou Xiaochen , Zhao Yanhong , Liu Kuisheng , Bai Dandan , Jia Yanping , Han Xiaoxiao , Sheng Yifan , Yin Jiqing , Wang Hong , Gao Shuai , Liu Wenqiang , Gao Shaorong
Published: August 2023

Somatic cell nuclear transfer (SCNT) can reprogram differentiated somatic cells into totipotency. Although pre-implantation development of SCNT embryos has greatly improved, most SCNT blastocysts are still arrested at the peri-implantation stage, and the underlying mechanism remains elusive. Here, we develop a 3D in vitro culture system for SCNT peri-implantation embryos and discover that persistent Wnt signals block the naïve-to-primed pluripotency transition of epiblasts with aberrant H3K27me3 occupancy, which in turn leads to defects in epiblast transformation events and subsequent implantation failure. Strikingly, manipulating Wnt signals can attenuate the pluripotency transition and H3K27me3 deposition defects in epiblasts and achieve up to a 9-fold increase in cloning efficiency. Finally, single-cell RNA-seq analysis reveals that Wnt inhibition markedly enhances the lineage developmental trajectories of SCNT blastocysts during peri-implantation development. Overall, these findings reveal diminished potentials of SCNT blastocysts for lineage specification and validate a critical peri-implantation barrier for SCNT embryos.
Continuous-Time Games with Imperfect and Abrupt Information
Authors: Bernard Benjamin
Published: August 2023

This paper studies two-player games in continuous time with imperfect public monitoring, in which information may arrive both gradually and continuously, governed by a Brownian motion, and abruptly and discontinuously, according to Poisson processes. For this general class of two-player games, we characterize the equilibrium payoff set via a convergent sequence of differential equations. The differential equations characterize the optimal trade-off between value burnt through incentives related to Poisson information and the noisiness of incentives related to Brownian information. In the presence of abrupt information, the boundary of the equilibrium payoff set may not be smooth outside the set of static Nash payoffs. Equilibrium strategies that attain extremal payoff pairs as well as their intertemporal incentives are elicitable from the limiting solution. The characterization is new even when information arrives through Poisson events only.
Health-related quality of life, remission and low lupus disease activity state in patients with systemic lupus erythematosus
Authors: Thibault Thomas , Rajillah Abdessamad , Bourredjem Abderrahmane , Corneloup Marie , Maurier François , Wahl Denis , Muller Geraldine , Aumaitre Olivier , Sève Pascal , Blaison Gilles , Besancenot Jean-François , Martin Thierry , Magy-Bertrand Nadine , Samson Maxime , Arnaud Laurent , Amoura Zahir , Devilliers Hervé ,   the EQUAL Study Group, Besancenot Jean-François , Bielefeld Philip , Berthier Sabine , Audia Sylvain , Lorcerie Bernard , Bonnotte Bernard , Samson Maxime , Vinit Julien , Ruault Sabine Meaux , Gil Helder , Broussolle Christiane , Varron Loig , Le Roux Karine , Zuily Stephane , Kaminsky Pierre , Mohamed Shirine , Muller Daniela Ibba , de Korwin Jean-Dominique , Guichard Jean-François , Marianetti Paola , Imbert Gaelle Guettrot
Published: August 2023

Objectives

To measure the association between SLE remission and scores of patients-reported outcome (PRO) measures.

Methods

We performed a prospective cohort study of SLE patients with a 2-year follow-up, using Lupus Patient-Reported Outcome (LupusPRO), Lupus Quality of Life (LupusQoL), Systemic Lupus Erythematosus Quality of Life (SLEQOL) and 36-item Short Form (SF-36) questionnaires. Remission was defined as remission off treatment (ROFT) and remission on treatment (RONT) according to the definitions of remission in SLE consensus. Mixed models accounting for repeated measures were used to compare groups as follow: ROFT and RONT vs no remission and lupus low disease activity state (LLDAS) vs no LLDAS.

Results

A total of 1478 medical visits and 2547 PRO questionnaires were collected during the follow-up from the 336 recruited patients. A between-group difference in PRO scores reaching at least 5 points on a 0–100 scale was obtained in the following domains: lupus symptoms (LLDAS: +5 points on the 0–100 scale, RONT: +9, ROFT: +5), lupus medication (LLDAS: +5, RONT: +8, ROFT: +9), pain vitality (LLDAS: +6, RONT: +9, ROFT: +6) of LupusPRO; role emotional (LLDAS: +5, RONT: +8), role physical (RONT: +7 and ROFT: +7), bodily pain (RONT: +6), mental health (RONT: +5) and social functioning (RONT: +6) of SF-36. In contrast, a between-group difference reaching at least 5 points was not achieved for any of the LupusQoL and SLEQOL domains.

Conclusions

RONT, ROFT and LLDAS were associated with significant and clinically relevant higher QoL in most PRO domains of the LupusPRO (disease specific) and SF-36 (generic) questionnaires, but not with LupusQoL and SLEQOL disease-specific questionnaires.
The Protestants’ Dilemma: When Cultural Mismatches Shape Deliberate Action
Authors: Tien Grace
Published: August 2023

Drawing from an ethnographic study and 63 interviews of Protestant professionals in the workplace, this article develops a conceptualization of how a cultural mismatch—defined here as a moral conflict between actors’ beliefs and values and their contextual norms and practices—catalyzes actors to strategically and deliberately shape future lines of action. In this study, a range of Protestant executives, professionals, and workers in China experience a cultural mismatch and respond in a number of ways. This study builds on accounts of culture in action to argue that when actors’ values and beliefs conflict with their organizational context, such cultural mismatches can shape action in not only unconscious, automatic ways or as post hoc justifications, as much of the extant scholarship has emphasized, but can also deliberately shape future lines of irrational, strategic, and creative action.
How Important Is Budding Speciation for Comparative Studies?
Authors: Caetano Daniel S , Quental Tiago B
Published: August 2023

The acknowledgment of evolutionary dependence among species has fundamentally changed how we ask biological questions. Phylogenetic models became the standard approach for studies with 3 or more lineages, in particular those using extant species. Most phylogenetic comparative methods (PCMs) translate relatedness into covariance, meaning that evolutionary changes before lineages split should be interpreted together whereas after the split lineages are expected to change independently. This clever realization has shaped decades of research. Here, we discuss one element of the comparative method often ignored or assumed as unimportant: if nodes of a phylogeny represent the dissolution of the ancestral lineage into two new ones or if the ancestral lineage can survive speciation events (i.e., budding). Budding speciation is often reported in paleontological studies, due to the nature of the evidence for budding in the fossil record, but it is surprisingly absent in comparative methods. Here, we show that many PCMs assume that divergence happens as a symmetric split, even if these methods do not explicitly mention this assumption. We discuss the properties of trait evolution models for continuous and discrete traits and their adequacy under a scenario of budding speciation. We discuss the effects of budding speciation under a series of plausible evolutionary scenarios and show when and how these can influence our estimates. We also propose that long-lived lineages that have survived through a series of budding speciation events and given birth to multiple new lineages can produce evolutionary patterns that challenge our intuition about the most parsimonious history of trait changes in a clade. We hope our discussion can help bridge comparative approaches in paleontology and neontology as well as foster awareness about the assumptions we make when we use phylogenetic trees.