bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification and quantification of viable Lacticaseibacillus rhamnosus in probiotics using validated PMA-qPCR method
Authors: Guo Lizheng , Ze Xiaolei , Feng Huifen , Liu Yiru , Ge Yuanyuan , Zhao Xi , Song Chengyu , Jiao Yingxin , Liu Jiaqi , Mu Shuaicheng , Yao Su
Published: January 2024

The identification and quantification of viable bacteria at the species/strain level in compound probiotic products is challenging now. Molecular biology methods, e.g., propidium monoazide (PMA) combination with qPCR, have gained prominence for targeted viable cell counts. This study endeavors to establish a robust PMA-qPCR method for viable Lacticaseibacillus rhamnosus detection and systematically validated key metrics encompassing relative trueness, accuracy, limit of quantification, linear, and range. The inclusivity and exclusivity notably underscored high specificity of the primers for L. rhamnosus, which allowed accurate identification of the target bacteria. Furthermore, the conditions employed for PMA treatment were fully verified by 24 different L. rhamnosus including type strain, commercial strains, etc., confirming its effective discrimination between live and dead bacteria. A standard curve constructed by type strain could apply to commercial strains to convert qPCR Cq values to viable cell numbers. The established PMA-qPCR method was applied to 46 samples including pure cultures, probiotics as food ingredients, and compound probiotic products. Noteworthy is the congruity observed between measured and theoretical values within a 95% confidence interval of the upper and lower limits of agreement, demonstrating the relative trueness of this method. Moreover, accurate results were obtained when viable L. rhamnosus ranging from 103 to 108 CFU/mL. The comprehensive appraisal of PMA-qPCR performances provides potential industrial applications of this new technology in quality control and supervision of probiotic products.
Global brain analysis of minor hallucinations in Parkinson’s disease using EEG and MRI data
Authors: Liu Chuan , Qu Liangcheng , Li Qixue , Cao Yiting , Shi Jingping , Yu Chao , Liu Weiguo , Yin Kuiying
Published: January 2024

Introduction

Visual hallucination is a prevalent psychiatric disorder characterized by the occurrence of false visual perceptions due to misinterpretation in the brain. Individuals with Parkinson’s disease often experience both minor and complex visual hallucinations. The underlying mechanism of complex visual hallucinations in Parkinson’s patients is commonly attributed to dysfunction in the visual pathway and attention network. However, there is limited research on the mechanism of minor hallucinations.

Methods

To address this gap, we conducted an experiment involving 13 Parkinson’s patients with minor hallucinations, 13 Parkinson’s patients without hallucinations, and 13 healthy elderly individuals. We collected and analyzed EEG and MRI data. Furthermore, we utilized EEG data from abnormal brain regions to train a machine learning model to determine whether the abnormal EEG data were associated with minor hallucinations.

Results

Our findings revealed that Parkinson’s patients with minor hallucinations exhibited excessive activation of cortical excitability, an imbalanced interaction between the attention network and the default network, and disruption in the connection between these networks. These findings is similar to the mechanism observed in complex visual hallucinations. The visual reconstruction of one patient experiencing hallucinations yields results that differ from those observed in subjects without such symptoms.

Discussion

The visual reconstruction results demonstrated significant differences between Parkinson’s patients with hallucinations and healthy subjects. This suggests that visual reconstruction techniques may offer a means of evaluating hallucinations.
The effectiveness of combined resection and radiotherapy for primary pineal malignant melanoma: a systematic review
Authors: Zhang Jibo , Wei Zixuan , Chen Jincao
Published: January 2024

Objective

To evaluate the effectiveness of combined resection and radiotherapy (CRAR) for the treatment of primary pineal malignant melanoma (PPMM).

Methods

Relevant studies were identified through a literature search in PubMed, Embase, and Web of Science from 1899 to September 1, 2023. Then we further screened the literature according to the updated PRISMA 2020 guidelines. The article information, patient information, treatment, and survival rate were analyzed. The primary outcome measures the survival rate of CRAR compared with the overall patients and the patients without treatment. Secondary outcome measures operation methods, radiotherapy methods, and dose.

Results

In total, 28 published articles were recorded. Among them, 35.71% (10/28) articles were on CRAR. The median overall survival, CRAR, and no treatment survival were 65, 88, and 12 weeks, respectively. The median overall survival of CRAR was demonstrably better than that of no treatment (p < 0.0001) and overall survival, even with p = 0.1177. Most of the operations adopted a supracerebellar infratentorial approach, and stereotactic radiation to tumor bed usually ranged between 50 and 60 Gy. Small dose and multiple fractions was the most popular radiotherapy method.

Conclusion

Currently, CRAR, compared with other treatments, is more beneficial to prolonging the survival of PPMM patients. However, many more clinical cases are needed to verify it as the best treatment approach.
Magnetic resonance imaging in acute meningoencephalitis of viral and unknown origin: frequent findings and prognostic potential
Authors: Abbuehl Lena S. , Branca Mattia , Ungureanu Anamaria , Federspiel Andrea , Leib Stephen L. , Bassetti Claudio L. A. , Hakim Arsany , Dietmann Anelia
Published: January 2024

Background

Magnetic resonance imaging (MRI) findings in meningoencephalitis have mainly been described in terms of their diagnostic value rather than their prognostic potential, except for herpes simplex virus (HSV) encephalitis. The aims of our study were to describe frequency and anatomic locations of MRI abnormalities specific to limbic, circadian and motor systems in a cohort of meningoencephalitis patients, as well as to investigate the prognostic value of these MRI findings.

Methods

A secondary, selective analysis of a retrospective database including all meningitis, meningoencephalitis and encephalitis cases treated between 2016 and 2018 in the University hospital of Bern, Switzerland was performed. Patients with meningitis of any cause, bacterial or autoimmune causes of encephalitis were excluded.

Results

MRI scans and clinical data from 129 meningoencephalitis cases found that the most frequent causes were tick-borne encephalitis (TBE, 42%), unknown pathogens (40%), VZV (7%), and HSV1 (5%). At discharge, median modified Rankin Score (mRS) was 3 (interquartile range, IQR, 1), 88% of patients had persisting signs and symptoms. After a median of 17 months, median Glasgow Outcome Score (GOS) was 5 (IQR 1), 39% of patients still had residual signs or symptoms. All patients with HSV, 27% with TBE and 31% of those with meningoencephalitis of unknown etiology had fluid-attenuated inversion recovery (FLAIR) and to a lesser extent diffusion-weighted imaging (DWI) lesions in their initial MRI, with highly overlapping anatomical distribution. In one fifth of TBE patients the limbic system was affected. Worse outcome was associated with presence of DWI and/or FLAIR lesions and lower normalized apparent diffusion coefficient (ADC) signal intensities.

Conclusion

Presence of FLAIR lesions, restricted diffusion as well as the extent of ADC hypointensity in initial MRI are parameters which might be of prognostic value regarding the longterm clinical outcome for patients with meningoencephalitis of viral and of unknown origin. Although not described before, affection of limbic structures by TBE is possible as shown by our results: A substantial proportion of our TBE patients had FLAIR signal abnormalities in these regions.
Study from the United States: increased prevalence of kidney stones in patients with high weight-adjusted waist index
Authors: Gui Zhaohua , Yu Longshan , Chen Yan , Zhang Mingxun , He Jie , Hao Yunwu
Published: January 2024

Objective

Using data from NHANES 2007–2018, to examine the association between WWI (weight-adjusted waist index) index and prevalence of kidney stones.

Methods

Using multiple logistic regression analysis of the National Health and Nutrition Examination Survey (NHANES) 2007–2018, we evaluated the association between WWI index and the prevalence of kidney stones, followed by subgroup analysis of sensitive populations. Smooth curve fitting was used to determine whether there was a non-linear relationship between the WWI index and kidney stone prevalence, and threshold effect analysis was used to test this relationship.

Results

Among 29,280 participants, 2,760 self-reported renal calculi. After adjustment for all confounders, there was a positive association between WWI and kidney stone prevalence (OR = 1.20, 95% CI: 1.12, 1.28), and this positive association was stronger with increasing WWI (and P = 0.01 for trend). Our results indicate a non-linear positive correlation between WWI index and kidney stones, with the saturation threshold effect analysis and the most important threshold value at 11.02. According to subgroup analysis, WWI showed the strongest association with kidney stone prevalence in participants aged 20–39 years, males, other US ethnic groups, and participants without hypertension and diabetes.

Conclusion

Increased WWI is positively associated with increased incidence of kidney stones, and increased WWI is a high risk for kidney stones that should be treated with caution. This association should be more pronounced in people between the ages of 20 and 39 years, in men, in other US ethnic populations, and in participants who do not have hypertension or diabetes.
Association between dietary inflammatory index and chronic kidney disease in middle-aged and elderly populations
Authors: Guo Meiqian , Lei Yi , Liu Xueqing , Li Xiang , Xu Yong , Zheng Donghui
Published: January 2024

Background

A link between food-induced inflammation and common chronic diseases has been identified in studies. However, there was uncertainty about the influence of dietary inflammatory potential on the risk of chronic kidney disease (CKD) among middle-aged and older groups. Our research aimed to examine the connection between dietary inflammatory index (DII) to CKD in people aged 40 years and older.

Methods

This study comprised ten cycles of the National Health and Nutrition Examination Survey (NHANES) from 1999 to 2018. Linear associations of DII with CKD, low-eGFR, and albuminuria were examined using multiple logistic regression, whereas non-linear associations were assessed by smoothed curve fitting. Besides, we conducted subgroup analyses and interaction tests.

Results

Of the 23,175 middle-aged and older individuals, a total of 5,847 suffered from CKD, making up 25.23% of all participants. After adjustment for all covariates, we found that increased DII scores were positive with an increased hazard of CKD (OR = 1.08, 95% CI: 1.05, 1.10, p < 0.0001), and the same was shown between DII and low-eGFR (OR = 1.16, 95% CI: 1.13, 1.19, p < 0.0001). After further converting DII into categorical variables, the above relationship still existed. These relations were consistent in different ages, genders, BMI, whether smoking, whether suffering from hypertension, and whether suffering from diabetes, with no significant stratification differences (all P for interaction >0.05). Surprisingly, we did not find a statistically significant correlation of DII to albuminuria after complete adjustment for covariates (OR = 1.02, 95% CI: 1.00, 1.05, p = 0.0742). Even when DII was considered as a categorical variable, this relation was still not statistically significant. Furthermore, we found an association in the shape of a U between DII and low-eGFR in the fully adjusted model, with a turning point at a DII of 1.6.

Conclusion

Our findings indicated that middle-aged and older persons with greater levels of DII had a significantly higher risk of CKD.
Pharmacologic inhibition of somatostatin receptor 2 to restore glucagon counterregulation in diabetes
Authors: Hoffman Emily G. , D’Souza Ninoschka C. , Liggins Richard T. , Riddell Michael C.
Published: January 2024

Glucose homeostasis is primarily maintained by pancreatic hormones, insulin and glucagon, with an emerging role for a third islet hormone, somatostatin, in regulating insulin and glucagon responses. Under healthy conditions, somatostatin secreted from pancreatic islet δ-cells inhibits both insulin and glucagon release through somatostatin receptor- induced cAMP-mediated downregulation and paracrine inhibition of β- and α-cells, respectively. Since glucagon is the body’s most important anti-hypoglycemic hormone, and because glucagon counterregulation to hypoglycemia is lost in diabetes, the study of somatostatin biology has led to new investigational medications now in development that may help to restore glucagon counterregulation in type 1 diabetes. This review highlights the normal regulatory role of pancreatic somatostatin signaling in healthy islet function and how the inhibition of somatostatin receptor signaling in pancreatic α-cells may restore normal glucagon counterregulation in diabetes mellitus.
Revealing the transitory and local effect of zebularine on development and on proteome dynamics of Salix purpurea
Authors: Pagano Andrea , Gomes Carolina , Timmerman Evy , Sulima Paweł , Przyborowski Jerzy Andrzej , Kruszka Dariusz , Impens Francis , Paiva Jorge Almiro Pinto
Published: January 2024

Introduction

DNA methylation plays major roles in the epigenetic regulation of gene expression, transposon and transcriptional silencing, and DNA repair, with implications in developmental processes and phenotypic plasticity. Relevantly for woody species, DNA methylation constitutes a regulative layer in cell wall dynamics associated with xylogenesis. The use of methyltransferase and/or demethylase inhibitors has been proven informative to shed light on the methylome dynamics behind the regulation of these processes.

Methods

The present work employs the cytidine analog zebularine to inhibit DNA methyltransferases and induce DNA hypomethylation in Salix purpurea plantlets grown in vitro and in soil. An integrative approach was adopted to highlight the effects of zebularine on proteomic dynamics, revealing age-specific (3 weeks of in vitro culture and 1 month of growth in soil) and tissue-specific (stem and root) effects.

Results and discussion

After 3 weeks of recovery from zebularine treatment, a decrease of 5-mC levels was observed in different genomic contexts in the roots of explants that were exposed to zebularine, whereas a functionally heterogeneous subset of protein entries was differentially accumulated in stem samples, including entries related to cell wall biosynthesis, tissue morphogenesis, and hormonal regulation. Significant proteomic remodeling was revealed in the development from in vitro to in-soil culture, but no significant changes in 5-mC levels were observed. The identification of tissue-specific proteomic hallmarks in combination with hypomethylating agents provides new insights into the role of DNA methylation and proteome in early plant development in willow species. Proteomic data are available via ProteomeXchange with identifier PXD045653. WGBS data are available under BioProject accession PRJNA889596.
Lying about money and game points by men and women and its relation to the Self-Reported Lying Scale
Authors: Elaad Eitan , Kochav Ron , Elkouby Tamar
Published: January 2024

Introduction

The present study was designed to examine the effect of monetary and non-monetary endowment on lying by men and women in the Ultimatum Game. Another goal was to examine to what extent the Self-Reported Lying Scale (SRLS), described here for the first time, predicts lying in the Ultimatum Game.

Methods

Examinees (162, 82 women) were allocated to four experimental conditions in a 2 × 2 factorial design. Two endowment conditions (money and game points) were crossed with two sex conditions (men and women). Participants underwent an Ultimatum Game in which they were permitted to conceal part of the endowment from an unidentified partner. Finally, participants completed the SRLS.

Results

The results indicated that more cash than points were concealed from the partner, and men concealed more of their endowment than women. We further defined fake fairness in sharing that combined hiding a more significant portion of the endowment from the partner while presenting fair sharing of the remaining award. We found more fake fairness when money was shared than when points were concealed. Fake fairness is more significant for men than for women. For money and points alike, concealment was predicted by the global score of the SRLS and its five subscales (self-assessed lying ability, lie detection ability, the use of reason in lying, lie acceptability, and lie frequency).

Discussion

It was suggested that a monetary endowment is more sensitive to lying than game points and involves more fake fairness. Nevertheless, the differences are quantitative, and the same response pattern exists in the two endowment conditions. Replacing money with points is a proper solution whenever a monetary endowment presents difficulties. It was further suggested that sex differences exist in lying using an asymmetric information UG, where proposers were permitted to mislead responders about their endowment. Finally, the SRLS may contribute to a better understanding of the question of who lies.
The relationship between childhood interpersonal and non-interpersonal trauma and autobiographical memory: a systematic review
Authors: Borrelli Giovanni , Lamberti Zanardi Annachiara , Scognamiglio Claudia , Cinquegrana Vincenza , Perrella Raffaella
Published: January 2024

Childhood trauma can have negative effects on several domains of mental functioning, including Autobiographical Memory (AM). Conflicting results emerge in the scientific literature regarding the effects of childhood trauma on AM. In this review, we explored the relationship between the childhood trauma and AM, classifying childhood trauma as interpersonal, non-interpersonal and overall (interpersonal and non-interpersonal). We carried out a systematic literature review, following the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA statement). From searching the PubMed, Scopus, and Web of Science databases, we identified 48 studies conducted from 2014 to 2023, which were included when they: (a) were written in English, (b) investigated the relationship between AM and childhood trauma, (c) included a sample of children, adolescents, or adults who had experienced childhood interpersonal and/or non-interpersonal trauma. Of the 48 eligible studies, 29 referred to trauma of an interpersonal nature, 12 to trauma of a non-interpersonal nature, and 7 to overall trauma. Regarding the relationship between childhood trauma and AM, 24 studies found a negative relationship between childhood interpersonal trauma and AM; among the articles on non-interpersonal trauma, 10 studies found no relevant relationship; in the studies on overall trauma, 4 articles found negative relationship between overall trauma and AM. The literature explored in our systematic review supports the prevalence of a negative relationship between interpersonal childhood trauma and AM. This relationship is present regardless of psychiatric disorders (e.g., Depression, Post Traumatic Stress Disorder, and Personality Disorders), and in the presence of the latter, AM results even more fragmented. Future research should use more accurate methodologies in identifying and classifying childhood trauma in order to more precisely determine its effect on AM.
Study protocol – elucidating the neural correlates of functional remediation for older adults with bipolar disorder
Authors: Montejo Laura , Sole Brisa , Fortea Lydia , Jimenez Esther , Martinez-Aran Anabel , Martinez-Heras Eloy , Sanchez-Moreno Jose , Ortuño Maria , Pariente Jose , Solanes Aleix , Torrent Carla , Vilajosana Enric , De Prisco Michele , Vieta Eduard , Radua Joaquim
Published: January 2024

Introduction

Beyond mood abnormalities, bipolar disorder (BD) includes cognitive impairments that worsen psychosocial functioning and quality of life. These deficits are especially severe in older adults with BD (OABD), a condition expected to represent most individuals with BD in the upcoming years. Restoring the psychosocial functioning of this population will thus soon represent a public health priority. To help tackle the problem, the Bipolar and Depressive Disorders Unit at the Hospital Clínic of Barcelona has recently adapted its Functional Remediation (FR) program to that population, calling it FROA-BD. However, while scarce previous studies localize the neural mechanisms of cognitive remediation interventions in the dorsal prefrontal cortex, the specific mechanisms are seldom unknown. In the present project, we will investigate the neural correlates of FR-OABD to understand its mechanisms better and inform for potential optimization. The aim is to investigate the brain features and changes associated with FROA-BD efficacy.

Methods

Thirty-two individuals with OABD in full or partial remission will undergo a magnetic resonance imaging (MRI) session before receiving FR-OABD. After completing the FR-OABD intervention, they will undergo another MRI session. The MRI sessions will include structural, diffusion-weighted imaging (DWI), functional MRI (fMRI) with working memory (n-back) and verbal learning tasks, and frontal spectroscopy. We will correlate the pre-post change in dorsolateral and dorsomedial prefrontal cortices activation during the n-back task with the change in psychosocial functioning [measured with the Functioning Assessment Short Test (FAST)]. We will also conduct exploratory whole-brain correlation analyses between baseline or pre-post changes in MRI data and other clinical and cognitive outcomes to provide more insights into the mechanisms and explore potential brain markers that may predict a better treatment response. We will also conduct separate analyses by sex.

Discussion

The results of this study may provide insights into how FROA-BD and other cognitive remediations modulate brain function and thus could optimize these interventions.
Advancing ecological validity and clinical utility in virtual reality-based continuous performance test: exploring the effects of task difficulty and environmental distractors
Authors: Oh Hyang-Kyeong , Cho Yoon Jae , Kim Jae-Jin , Shin Bokyoung , Kim Soo-Jeong , Park Soobin , Seok Jeong-Ho , Kim Soyeon , Kim Eunjoo
Published: January 2024

Introduction

Researchers have highlighted concerns regarding the limited diagnostic utility and ecological validity of the Continuous Performance Test (CPT). Recent advancements in VR-based CPTs have attempted to address these concerns by simulating real-life scenarios and enhancing attention deficit hyperactivity disorder (ADHD) diagnosis; however, certain areas require improvement for obtaining reliable data from both healthy individuals and those with ADHD. To tackle these issues, we developed an enhanced VR-based CPT program featuring four distinct difficulty levels, advancing toward home-based assessment.

Method

Our feasibility study involved subjects without ADHD to establish a normative profile for VR-based CPT before extending it to ADHD assessment. Our sample included 20 Korean adults. They received a VR device with the VR-based CPT program installed and were asked to perform 1-2 blocks per day at home. Participants were instructed to complete 12 blocks over the subsequent 2 weeks. Psychological assessments and electroencephalograms (EEGs) were administered before and after the program. Post-study usability measures were also collected.

Result

Higher commission errors were notably evident in the “very high” difficulty level which featured complex stimuli and increased distraction. A notable correlation emerged between the overall distraction level and CPT accuracy, along with a significant link between intensity scores and commission errors. No significant differences were found in psychological assessment and there were no significant changes in the Theta-Beta Ratio (TBR) index before and after the program. The usability of our program was fair.

Discussion

The study reveals that the newly designed VR-CPT program, simulating diverse real-life environments and offering varying task difficulty levels, proved acceptable and feasible. The key point of our study was that the adjustment and segmentation of difficulty levels in the VR-based CPT were achieved, and that this effort was validated by examining the impact of different levels of difficulty on CPT measures. Implementing this experimental setup in a home-based environment increased ecological validity, as well as clinical utility. Limitations and suggested directions for further investigation are described in detail.
The AIP model as a theoretical framework for the treatment of personality disorders with EMDR therapy
Authors: De Jongh Ad , Hafkemeijer Laurian , Hofman Simon , Slotema Karin , Hornsveld Hellen
Published: January 2024

Research has shown that the impact of traumatic events and circumstances on individuals is cumulative and potentially has a wide range of harmful consequences, including negative consequences on mental health. One such consequence is the development of a personality disorder, a persistent mental condition characterized by a pronounced pattern of difficulties in impulse control, emotional regulation, cognitive functions, self-esteem, and interpersonal relationships. A wide array of studies indicates that the personal history of individuals with a personality disorder is often marked by exposure to traumatic events or other types of adverse childhood experiences (ACEs). Because existing treatments for personality disorders are usually long and costly, it is essential to continue exploring alternative and complementary interventions. Nowadays, knowledge and clinical experience in regard to personality disorders have been gained in addressing ACEs by processing memories of these events through eye movement desensitization and reprocessing (EMDR) therapy. In this paper, we present a theoretical framework for this treatment approach, based on Shapiro’s Adaptive Information Processing (AIP) model, describe its current empirical basis, and provide guidance on how to formulate a useful case conceptualization that can serve as a basis for the treatment of personality disorders with EMDR therapy. This approach is illustrated with a case example.
The relationship between major life events and non-suicidal self-injury among college students: the effect of rumination and body image
Authors: Qin Qian , Yang Guangni , Li Yue , Wu Wanchun , Wang Jianping , Chen Ziyao , Kong Xiaohua , Zhang Wei , Zou Hongyu
Published: January 2024

Background

Non-suicidal self-injury (NSSI) poses a growing risk to public health worldwide. While numerous studies have identified major life events as key risk factors for NSSI, the mechanisms by which emotional and cognitive problems mediate or moderate this relationship remain unclear. To enhance the understanding of this field, we will draw upon the cascade theory of self-injury and the benefits and barriers model, to examine the relationship between major life events and NSSI, as well as the effect of rumination and body image.

Methods

A sample of 2,717 college students (Mage = 19.81 years; SD = 1.09) participated in this study and anonymously completed the questionnaires. The moderated mediation model were conducted using Model 4 and Model 15 of the Process macro program in SPSS.

Results

The results showed that rumination mediated the positive relationship between major life events and NSSI. Furthermore, body image was found to moderate both the relationship between major life events and NSSI, as well as the relationship between rumination and NSSI.

Conclusion

The current findings suggest that rumination is an important mediator in the relationship between major life events and NSSI among college students. Teachers, parents, and researchers should recognize the important role of body image self-perceptions of college students and actively promote a healthy and accurate body image.
Post-traumatic stress and depression following disaster: examining the mediating role of disaster resilience
Authors: First Jennifer M.
Published: January 2024

The current study used structural equation modeling to examine the role of disaster resilience as a mediator between disaster exposure and post-traumatic stress and depressive symptoms among a sample of 625 U.S. adults who experienced a disaster event. Results found that disaster resilience mediated the relationship between disaster exposure as a predictor and depression and post-traumatic stress as dependent variables. These findings have important implications for understanding the mechanisms by which disaster resilience supports post-disaster mental health and can inform future disaster mental health interventions and practice models.
Examining associations of folic acid supplements administered to mothers during pre-conceptional and prenatal periods with autism spectrum disorders in their offspring: insights from a multi-center study in China
Authors: Jiang Yan , Guo Cuihua , Kuang Min , Lin Lizi , Xu Guifeng , Pan Ning , Weng Xuchu , Jing Jin , Shi Lei , Yi Quanying , Wang Xin
Published: January 2024

Objective

To investigate the relationship between maternal folic acid (FA) supplementation during the pre-conceptional and prenatal periods and the subsequent risk of autism spectrum disorder (ASD) in offspring.

Methods

A total of 6,049 toddlers aged 16–30 months were recruited from August 2016 to March 2017 for this cross-sectional study conducted in China. The parents of the enrolled toddlers provided information on maternal supplemental FA, socio-demographic information, and related covariates. Standard diagnostic procedures were implemented to identify toddlers with ASD.

Results

Among the 6,049 children included in the study, consisting of 3,364 boys with an average age of 22.7 ± 4.1 months, a total of 71 children (1.2%) were diagnosed with ASD. Mothers who did not consume FA supplements during the prenatal period were found to have a significantly increased risk of having offspring with ASD, in comparison to those who were exposed to FA supplements (odds ratio [OR] = 2.47). However, we did not find a similar association during the pre-conceptional period. Compared to mothers who consistently used FA supplements from pre-conception to the prenatal period, those who never used FA supplements were statistically significantly associated with a higher risk of ASD in their offspring (OR = 2.88).

Conclusion

This study indicated that providing continuous maternal FA supplementation during the pre-conceptional and prenatal periods may decrease the risk of ASD in offspring. The prenatal period is considered to be the most crucial time for intervention.
Operational low-flow forecasting using LSTMs
Authors: Deng Jing , Couasnon Anaïs , Dahm Ruben , Hrachowitz Markus , van Heeringen Klaas-Jan , Korving Hans , Weerts Albrecht , Taormina Riccardo
Published: January 2024

This study focuses on exploring the potential of using Long Short-Term Memory networks (LSTMs) for low-flow forecasting for the Rhine River at Lobith on a daily scale with lead times up to 46 days ahead. A novel LSTM-based model architecture is designed to leverage both historical observation and forecasted meteorological data to carry out multi-step discharge time series forecasting. The feature and target selection for this deep learning (DL) model involves evaluating the use of different spatial resolutions for meteorological forcing (basin-averaged or subbasin-averaged), the impact of incorporating past discharge observations, and the use of different target variables (discharge Q or time-differenced discharge dQ). Then, the model is trained using the ERA5 dataset as meteorological forcing, and employed for operational forecast with ECMWF seasonal forecast (SEAS5) data. The forecast results are compared to a benchmark process-based model, wflow_sbm. This study also explores the flexibility of the DL model by fine-tuning the pretrained model with limited SEAS5 dataset. Key findings from feature and target selection include: (1) opting for subbasin-averaged meteorological variables significantly improves model performance compared to a basin-averaged approach. (2) Utilizing dQ as the target variable greatly boosts short-term forecast accuracy compared to using Q, with a mean absolute error (MAE) of 25 m3 s−1 and mean absolute percentage error (MAPE) of 0.02 for the first lead time, ensuring reliability and accuracy at the onset of the forecast horizon. (3) While incorporating historical discharge improves the forecasting of Q, its impact on predicting dQ is less pronounced for short lead times. In the operational forecast with SEAS5, compared to the wflow_sbm model, the DL model exhibits skill in forecasting low flows as evidenced by Continuous Ranked Probability Skill Score (CRPSS) median values of all lead times above zero, and better accuracy in forecasting drought events within short lead times. The wflow_sbm model shows higher accuracy for longer lead times. In the exploration of fine-tuning approach, the fine-tuned model generates marginal short-term enhancements in forecasting low-flow events over a non-fine-tuned model. Overall, this study contributes to advancing the field of low-flow forecasting using deep learning approach.
Efficacy and safety of intravenous mesenchymal stem cells for ischemic stroke patients, a systematic review and meta-analysis
Authors: Hassanein Maged Elsayed , Fayad Jaber , Shabana Jilan Ali , AlEdani Esraa M. , Hefnawy Mahmoud Tarek , Ghaith Hazem S. , Negida Ahmed
Published: January 2024

Background

Clinical trials have evaluated the efficacy of intravenous mesenchymal stem cells (MSCs) for acute and subacute ischemic stroke. Therefore, we conducted this meta-analysis to investigate the efficacy and safety of intravenous MSC treatments compared to placebo for acute and subacute ischemic stroke patients.

Methods

We searched PubMed, SCOPUS, Web of Science, and Cochrane CENTRAL for randomized controlled trials evaluating any clinical trials of intravenous MSCs for acute and subacute ischemic stroke patients. The efficacy outcomes of this study were the rates of improvement in National Institutes of Health Stroke Scale (NIHSS) scores, good scores on the modified Rankin Scale (mRS), and Barthel Index (BI) scores, while the safety outcomes were the rates of mortality and stroke recurrence. We compared intravenous MSC and placebo treatments on a fixed-effect meta-analysis model in R software.

Results

Four randomized controlled studies involving 97 patients were included in the analysis. In the meta-analysis, MSC treatments were superior to placebo treatments in good mRS (MD −0.95, 95% CI [−1.39, −0.52]) or BI (MD 21.36, 95% CI [9.96, 32.75]) scores, and MSC treatments were not superior to placebo treatments in the rate of improvement of the NIHSS scores (MD −1.81, 95% CI [−4.123, 0.494]). MSCs were associated with neither decreased mortality nor stroke recurrence (risk ratio 0.58 and 0.59, respectively; p-value = 0.51 and p-value = 0.533, respectively).

Conclusion

For patients with acute and subacute ischemic stroke who are eligible for further damage to neural tissue, MSCs achieve high efficacy and acceptable safety.

Systematic review registration

Prospero, unique ID: CRD42023457655.
Rumen-protected methionine supplementation alters lipid profile of preimplantation embryo and endometrial tissue of Holstein cows
Authors: Stella Stephanie L. , Guadagnin Anne R. , Velasco-Acosta Diego A. , Ferreira Christina R. , Rubessa Marcello , Wheeler Matthew B. , Luchini Daniel , Cardoso Felipe C.
Published: January 2024

Our objective is to evaluate the effects of feeding rumen-protected Met (RPM) throughout the transition period and early lactation on the lipid profile of the preimplantation embryos and the endometrial tissue of Holstein cows. Treatments consisted of feeding a total mixed ration with top-dressed RPM (Smartamine® M, Adisseo, Alpharetta, GA, United States; MET; n = 11; RPM at a rate of 0.08% of DM: Lys:Met = 2.8:1) or not (CON; n = 9, Lys:Met = 3.5:1). Endometrial biopsies were performed at 15, 30, and 73 days in milk (DIM). Prior to the endometrial biopsy at 73 DIM, preimplantation embryos were harvested via flushing. Endometrial lipid profiles were analyzed using multiple reaction monitoring-profiling and lipid profiles of embryos were acquired using matrix assisted laser desorption/ionization mass spectrometry. Relative intensities levels were used for principal component analysis. Embryos from cows in MET had greater concentration of polyunsaturated lipids than embryos from cows in CON. The endometrial tissue samples from cows in MET had lesser concentrations of unsaturated and monounsaturated lipids at 15 DIM, and greater concentration of saturated, unsaturated (specifically diacylglycerol), and monounsaturated (primarily ceramides) lipids at 30 DIM than the endometrial tissue samples from cows in CON. In conclusion, feeding RPM during the transition period and early lactation altered specific lipid classes and lipid unsaturation level of preimplantation embryos and endometrial tissue.
Comparative pharmacokinetics of free doxorubicin and a liposomal formulation in cats following intravenous administration
Authors: Liu Yu , Chen Sumeng , Wen Zeyu , Meng Jinyan , Yang Yuxin , Zhang Yang , Wang Jianzhong , Cao Xingyuan
Published: January 2024

Doxorubicin, a potent chemotherapeutic agent used extensively in cancer treatment, displays complex pharmacokinetic behavior, especially across various formulations. With a rising incidence of cancer cases in cats, understanding the drug’s pharmacokinetics in feline subjects remains a critical yet unexplored area. Hence, this study investigated the pharmacokinetic profile of doxorubicin after slow intravenous administration of doxorubicin hydrochloride (DOX·HCl) or doxorubicin hydrochloride pegylated liposome (DOX·HCl-PLI) in twelve cats at a single dose of 20 mg/m2. Blood samples collected at pretreatment time (0 h) and over 192 h were analyzed using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS/MS). The obtained pharmacokinetic parameters of doxorubicin revealed significant differences between the two formulations and were as follows: elimination half-life (T1/2λz) of 5.00 ± 3.20 h (DOX·HCl) and 17.62 ± 8.13 h (DOX·HCl-PLI), area under the concentration/time curve from 0 to last point (AUClast) of 0.67 ± 0.12 μg hr./mL (DOX·HCl) and 783.09 ± 267.29 μg hr./mL (DOX·HCl-PLI), and total body clearance (CL_obs) of 27098.58 ± 5205.19 mL/h/m2 (DOX·HCl) and 28.65 ± 11.09 mL/h/m2 (DOX·HCl-PLI). Additionally, differences were also detected in the apparent volume of distribution (Vz_obs) with 178.56 ± 71.89 L/m2 (DOX·HCl) and 0.64 ± 0.20 L/m2 (DOX·HCl-PLI), and the maximum plasma concentration (Cmax) with 2.25 ± 0.30 μg/mL (DOX·HCl) and 24.02 ± 5.45 μg/mL (DOX·HCl-PLI). Notably, low concentration of doxorubicinol, the metabolite of doxorubicin, was detected in plasma after administration of DOX·HCl, with even less present when DOX·HCl-PLI was administered. This investigation provides valuable insights into the distinct pharmacokinetic behaviors of DOX·HCl and DOX·HCl-PLI in cats, contributing essential groundwork for future studies and potential clinical applications in feline oncology.
Atom Filtering Algorithm and GPU-Accelerated Calculation of Simulation Atomic Force Microscopy Images
Authors: Amyot Romain , Kodera Noriyuki , Flechsig Holger
Published: January 2024

Simulation of atomic force microscopy (AFM) computationally emulates experimental scanning of a biomolecular structure to produce topographic images that can be correlated with measured images. Its application to the enormous amount of available high-resolution structures, as well as to molecular dynamics modelling data, facilitates the quantitative interpretation of experimental observations by inferring atomistic information from resolution-limited measured topographies. The computation required to generate a simulated AFM image generally includes the calculation of contacts between the scanning tip and all atoms from the biomolecular structure. However, since only contacts with surface atoms are relevant, a filtering method shall highly improve the efficiency of simulated AFM computations. In this report, we address this issue and present an elegant solution based on graphics processing unit (GPU) computations that significantly accelerates the computation of simulated AFM images. This method not only allows for the visualization of biomolecular structures combined with ultra-fast synchronized calculation and graphical representation of corresponding simulated AFM images (live simulation AFM), but, as we demonstrate, it can also reduce the computational effort during the automatized fitting of atomistic structures into measured AFM topographies by orders of magnitude. Hence, the developed method will play an important role in post-experimental computational analysis involving simulated AFM, including expected applications in machine learning approaches. The implementation is realized in our BioAFMviewer software (ver. 3) package for simulated AFM of biomolecular structures and dynamics.
Recording, Processing, and Reproduction of Vibrations Produced by Impact Noise Sources in Buildings
Authors: Dolezal Franz , Reichenauer Andreas , Wilfling Armin , Neusser Maximilian , Prislan Rok
Published: January 2024

Several studies on the perception of impact sounds question the correlation of standardized approaches with perceived annoyance, while more recent studies have come to inconsistent conclusions. All these studies neglected the aspect of whole-body vibrations, which are known to be relevant for the perception of low-frequency sound and can be perceived especially in lightweight constructions. Basically, the contribution of vibrations to impact sound annoyance is still unknown and could be the reason for the contradictory results. To investigate this aspect, we measured vibrations on different types of floors under laboratory conditions and in situ. For this purpose, a vibration-sensing device was developed to record vibrations more cost-effectively and independently of commercial recording instruments. The vibrations of predefined impact sequences were recorded together with the sound field using a higher-order ambisonics microphone. In addition, a vibration exposure device was developed to expose the test objects to the exact vibrations that occur in the built environment. The vibration exposure device is integrated into the ambisonics reproduction system, which consists of a large number of loudspeakers in a spherical configuration. The article presents the development and performance achieved using the vibration-sensing unit and the vibration exposure device. The study is relevant for conducting future impact sound listening tests under laboratory conditions, which can be extended to include the reproduction of vibrations.
Design of an Optical Path Scanning Control System in a Portable Fourier Transform Spectrometer Based on Adaptive Feedforward–Nonlinear Proportional-Integral Cascade Composite Control
Authors: Zhi Liangjie , Huang Min , Wen Qin , Gao Han , Han Wei
Published: January 2024

In order to obtain highly accurate infrared spectra, the optical path scanning control system in a portable Fourier transform spectrometer (FTS) must be able to realize highly stable reciprocal scanning. To address the positional localization and speed fluctuation problems of optical path scanning control systems, an adaptive feedforward–nonlinear PI cascade composite control algorithm (AF-NLPI) is proposed. A physical model of an optical path scanning control system is established. Moreover, an adaptive feedforward compensator using a dynamic forgetting factor is proposed, and it was combined with a nonlinear PI cascade controller to form a composite controller. The control parameters were tuned using the atomic orbital search algorithm. Further, the simulation and experimental results demonstrate that the AF-NLPI can effectively improve the control accuracy and anti-interference ability of an optical path scanning control system in a portable FTS with high feasibility and practicality. By setting the scanning stroke of the system to 8 mm and scanning at 10 mm/s, the stability of the optical scanning speed reached 99.47% when controlled by the controller proposed in this paper, thus fulfilling the motion requirements for optical path scanning control systems.
Design, Manufacturing, and Open-Loop Control of a Soft Pneumatic Arm
Authors: García-Samartín Jorge Francisco , Rieker Adrián , Barrientos Antonio
Published: January 2024

Soft robots distinguish themselves from traditional robots by embracing flexible kinematics. Because of their recent emergence, there exist numerous uncharted territories, including novel actuators, manufacturing processes, and advanced control methods. This research is centred on the design, fabrication, and control of a pneumatic soft robot. The principal objective is to develop a modular soft robot featuring multiple segments, each one with three degrees of freedom. This yields a tubular structure with five independent degrees of freedom, enabling motion across three spatial dimensions. Physical construction leverages tin-cured silicone and a wax-casting method, refined through an iterative processes. PLA moulds that are 3D-printed and filled with silicone yield the desired model, while bladder-like structures are formed within using solidified paraffin wax-positive moulds. For control, an empirically fine-tuned open-loop system is adopted. This paper culminates in rigorous testing. Finally, the bending ability, weight-carrying capacity, and possible applications are discussed.
A Soft Sensor Model for Predicting the Flow of a Hydraulic Pump Based on Graph Convolutional Network–Long Short-Term Memory
Authors: Ji Shengfei , Li Wei , Wang Yong , Zhang Bo , Ng See-Kiong
Published: January 2024

The hydraulic pump plays a pivotal role in engineering machinery, and it is essential to continuously monitor its operating status. However, many vital signals for monitoring cannot be directly obtained in practical applications. To address this, we propose a soft sensor approach for predicting the flow signal of the hydraulic pump based on a graph convolutional network (GCN) and long short-term memory (LSTM). Our innovative GCN-LSTM model is intricately designed to capture both spatial and temporal interdependencies inherent in complex machinery, such as hydraulic pumps. We used the GCN to extract spatial features and LSTM to extract temporal features of the process variables. To evaluate the performance of GCN-LSTM in predicting the flow of a hydraulic pump, we construct a real-world experimental dataset with an actual hydraulic shovel. We further evaluated GCN-LSTM on two public datasets, showing the effectiveness of GCN-LSTM for predicting the flow of hydraulic pumps and other complex engineering operations.