bluuebunny/crossref_metadata_2025 huggingface dataset:
Interactions between the Public and Assistance Dog Handlers and Trainers
Authors: McManus Bronwyn , Good Gretchen , Yeung Polly
Published: November 2021

This research aimed to explore the experiences of handlers and trainers of disability assistance dogs in terms of the types of interactions they had with members of the Aotearoa NZ (NZ) public and how these interactions were perceived, interpreted, and managed. A qualitative method, guided by an interpretive approach and social constructionism, was utilised to collect data via semi-structured interviews with six handlers and six trainers of assistance dogs. Data were analysed using thematic analysis with the social model of disability as the theoretical base. Findings indicated that participants regularly faced a complex range of unique interactions due to various factors such as the public’s lack of knowledge and understanding of the dog’s role and right of access to public places. While participants encountered brief friendly comments about the dog and its role, other encounters involved long conversations, invasive personal questions, interference with their dogs, and denied access to businesses, cafés, restaurants, and public transport. These findings underpin the need to provide more education to the public on the etiquette of engaging with handlers and their assistance dogs and more support for businesses to understand the legal rights of handlers. Through education and support to change societal attitudes and remove structural barriers, disabled people using assistance dogs may be able to independently participate in community life and be fully included without hindrance.
A Randomized Clinical Trial Evaluating the Effect of an Oral Calcium Bolus Supplementation Strategy in Postpartum Jersey Cows on Mastitis, Culling, Milk Production, and Reproductive Performance
Authors: Menta Paulo R. , Fernandes Leticia , Poit Diego , Celestino Maria Luiza , Machado Vinicius S. , Neves Rafael C.
Published: November 2021

The objectives of this study were to evaluate the effects of a postpartum oral calcium supplementation strategy in multiparous Jersey cows on (1) the odds of clinical mastitis in the first 60 days in milk (DIM); (2) the odds of culling up to 60 DIM; (3) the risk of pregnancy in the first 150 DIM; (4) milk production in the first 15 weeks of lactation. A randomized clinical trial was performed in a dairy herd located in west Texas, United States. A total of 809 cows were used in the final analyses. Overall, postpartum oral calcium supplementation did not influence milk production, reproductive performance, or culling. Among second parity cows, oral calcium supplementation tended to decrease the odds of clinical mastitis in the first 60 DIM compared to controls; however, no differences were observed for cows in parities three and greater. To date, data evaluating the effect of postpartum oral calcium supplementation in multiparous Jersey cows are limited. In our study, oral calcium supplementation tended to reduce clinical mastitis in second parity cows. No positive benefits based on the reduction of culling, and improvement of milk production and reproductive performance were evident for the herd included in this study.
Age and Body Condition Influence the Post-Prandial Interleukin-1β Response to a High-Starch Meal in Horses
Authors: Suagee-Bedore Jessica , Shost Nichola , Miller Christian , Grado Luis , Bechelli Jeremy
Published: November 2021

Older horses and those prone to obesity may be at a higher risk for inflammation than younger and leaner counterparts. Previous research indicated a postprandial elevation in plasma concentrations of interleukin-1β (IL-1β), a pro-inflammatory cytokine, after consuming 1.2 g of non-structural carbohydrates/kilogram of body weight. However, these studies utilized horses of mixed age and body condition. The current study evaluated post-prandial IL-1β concentrations in horses specifically comparing lean to over-conditioned and middle aged to older. Our results suggest that at least two weeks of daily consumption of a high non-structural carbohydrate diet is required to induce a post-prandial increase in IL-1β concentrations in younger and leaner horses. In opposition to this, older and over-conditioned horses experience plasma increased on the first day of feeding and thereafter. Feeding management practices of older and over-conditioned individuals should emphasize lower non-structural carbohydrate intakes and further research should elucidate mechanisms of IL-1β activation.
Maternal Dietary Nitrate Supplementation Lowers Incidence of Stillbirth in Hyper Prolific Sows under Commercial Circumstances
Authors: van den Bosch Moniek , Bronsvoort Bram , Kemp Bas , van den Brand Henry
Published: November 2021

The objective of the current experiment was to investigate whether or not maternal dietary nitrate supplementation, a nitric oxide (NO) precursor, could reduce piglet losses under commercial circumstances. In the current experiment, 120 hyper prolific gilts and sows (Landrace x Yorkshire: Danbred) on a commercial farm in Denmark received either a control lactation diet or a lactation diet containing 0.1% of calcium nitrate (containing 63.1% of nitrate) from approximately 5 days pre-farrowing until day 4 of lactation. The number of piglets born total, alive, and stillborn, as well as birth weights, weights after cross-fostering (approximately 1 day of age), 24 h after cross-fostering, day 3 of age, and at weaning was recorded. Placentas of sows were collected after expulsion and scored on redness. No effect of nitrate supplementation was found on piglet weight, piglet growth, placental redness score, and pre-weaning mortality during lactation. Maternal dietary nitrate supplementation decreased stillbirth percentage with 2.5% (9.9 vs. 7.4%; p = 0.05). It can be concluded that maternal dietary nitrate supplementation shows the potential to decrease the incidence of stillbirth in hyper prolific sows.
Raw Chickpea (Cicer arietinum L.) as a Substitute of Soybean Meal in Compound Feed for Broiler Chickens: Effects on Growth Performance, Lipid Metabolism, Fatty Acid Profile, Antioxidant Status, and Dietary Value of Muscles
Authors: Danek-Majewska Anna , Kwiecień Małgorzata , Winiarska-Mieczan Anna , Haliniarz Małgorzata , Bielak Agata
Published: November 2021

The aim of this study was to determine the effect of substitution of 50% of soybean meal protein with 310–350 g/kg diet of raw chickpea seed protein on the chemical composition, fatty acid profile, dietary value, and antioxidant status of breast and thigh muscles, as well as the antioxidant status of blood serum, in Ross 308 male broilers. In the 42-day experiment, one-day-old male broiler chicks were assigned to two nutritional groups (n = 100 in each, 20 birds in each group, and 5 replications). In the control group, 100% of protein in the feed was derived from soybean meal. In the experimental group, 310–350 g/kg protein from raw chickpea seeds was introduced. Data with a normal distribution were analyzed using the Student t-test, and the relationships between the traits were assessed with the use of Pearson’s correlation coefficients. p < 0.05 was considered statistically significant. The replacement with chickpea protein did not exert an impact on the final body weight, feed consumption, and feed conversion ratio compared to the control group. However, it induced changes in the color of the breast muscles (increased L* and b* values), and reduced the cholesterol content. The addition of chickpea seeds improved the fatty acid profile, mainly in the breast muscle. A decrease in the total SFA content and a higher level of unsaturated fatty acids (UFA), UFAs/saturated fatty acids (SFAs), polyunsaturated fatty acids (PUFAs), omega-3, and omega-6 were observed in the experimental group. Additionally, the chickpea-supplemented group exhibited better values of meat quality indicators (atherogenic index-AI; thrombogenic index–TI, ratio of saturated fatty acids to unsaturated fatty acids-S/P, n-6/n-3, hypocholesterolemic/Hypercholesterolemic ratio-h/H). It can be concluded that raw chickpea seeds are a good source of protein in broiler chicken nutrition, and can replace the traditionally used protein source (soybean meal), simultaneously exerting a positive effect on the dietary value of poultry meat and an expected enhancing impact on consumer health.
Third-Generation Cephalosporin Resistance in Intrinsic Colistin-Resistant Enterobacterales Isolated from Retail Meat
Authors: Odoi Justice Opare , Takayanagi Sayo , Yossapol Montira , Sugiyama Michiyo , Asai Tetsuo
Published: November 2021

Consumption of retail meat contaminated with antimicrobial-resistant (AMR) bacteria is a common route for transmitting clinically relevant resistant bacteria to humans. Here, we investigated the genotypic and phenotypic resistance profiles of intrinsic colistin-resistant (ICR) Enterobacterales isolated from retail meats. ICR Enterobacterales were isolated from 103 samples of chicken, 103 samples of pork, and 104 samples of beef purchased from retail shops in Japan, using colistin-containing media, and their antimicrobial susceptibility was examined. Serratia spp. (440 isolates) showed resistance to cefotaxime (19 isolates, 4.3%), tetracycline (15 isolates, 3.4%), and other antimicrobials (<1%). Hafnia spp. (136) showed resistance to cefotaxime (12 isolates, 8.6%), ceftazidime (four isolates, 2.9%), and tetracycline (two isolates, 1.4%). Proteus spp. (39) showed resistance to chloramphenicol (four isolates, 10.3%), sulfamethoxazole-trimethoprim (four isolates, 10.3%), cefotaxime (two isolates, 5.1%), kanamycin (two isolates, 5.1%), and gentamicin (one isolate, 2.6%). Cedecea spp. (22) were resistant to tetracycline (two isolates, 9.1%) whereas Morganella spp. (11) were resistant to tetracycline (four isolates, 36.4%) and chloramphenicol (one isolate, 9.2%). The resistance genes blafonA, blaACC, and blaDHA were detected in cefotaxime-resistant Serratia spp., Hafnia spp., and Morganella spp. isolates, respectively. This emergence of antimicrobial resistance in ICR Enterobacterales may pose a public health risk.
Assessing the Impact of Heat Treatment of Food on Antimicrobial Resistance Genes and Their Potential Uptake by Other Bacteria—A Critical Review
Authors: James Christian , Dixon Ronald , Talbot Luke , James Stephen J. , Williams Nicola , Onarinde Bukola A.
Published: November 2021

The dissemination of antibiotic resistance genes (ARGs) is a global health concern. This study identifies and critically reviews the published evidence on whether cooking (heating) food to eliminate bacterial contamination induces sufficient damage to the functionality of ARGs. Overall, the review found that there is evidence in the literature that Antimicrobial Resistant (AMR) bacteria are no more heat resistant than non-AMR bacteria. Consequently, recommended heat treatments sufficient to kill non-AMR bacteria in food (70 °C for at least 2 min, or equivalent) should be equally effective in killing AMR bacteria. The literature shows there are several mechanisms through which functional genes from AMR bacteria could theoretically persist in heat-treated food and be transferred to other bacteria. The literature search found sparce published evidence on whether ARGs may actually persist in food after effective heat treatments, and whether functional genes can be transferred to other bacteria. However, three publications have demonstrated that functional ARGs in plasmids may be capable of persisting in foods after effective heat treatments. Given the global impact of AMR, there is clearly a need for further practical research on this topic to provide sufficient evidence to fully assess whether there is a risk to human health from the persistence of functional ARGs in heat-treated and cooked foods.
Antimicrobial Susceptibility and Frequency of bla and qnr Genes in Salmonella enterica Isolated from Slaughtered Pigs
Authors: Calayag Alyzza Marie B. , Widmer Kenneth W. , Rivera Windell L.
Published: November 2021

Salmonella enterica is known as one of the most common foodborne pathogens worldwide. While salmonellosis is usually self-limiting, severe infections may require antimicrobial therapy. However, increasing resistance of Salmonella to antimicrobials, particularly fluoroquinolones and cephalosporins, is of utmost concern. The present study aimed to investigate the antimicrobial susceptibility of S. enterica isolated from pork, the major product in Philippine livestock production. Our results show that both the qnrS and the blaTEM antimicrobial resistance genes were present in 61.2% of the isolates. While qnrA (12.9%) and qnrB (39.3%) were found less frequently, co-carriage of blaTEM and one to three qnr subtypes was observed in 45.5% of the isolates. Co-carriage of blaTEM and blaCTX-M was also observed in 3.9% of the isolates. Antimicrobial susceptibility testing revealed that the majority of isolates were non-susceptible to ampicillin and trimethoprim/sulfamethoxazole, and 13.5% of the isolates were multidrug-resistant (MDR). MDR isolates belonged to either O:3,10, O:4, or an unidentified serogroup. High numbers of S. enterica carrying antimicrobial resistance genes (ARG), specifically the presence of isolates co-carrying resistance to both β-lactams and fluoroquinolones, raise a concern on antimicrobial use in the Philippine hog industry and on possible transmission of ARG to other bacteria.
Antibacterial, Immunomodulatory, and Lung Protective Effects of Boswelliadalzielii Oleoresin Ethanol Extract in Pulmonary Diseases: In Vitro and In Vivo Studies
Authors: Alotaibi Badriyah , Negm Walaa A. , Elekhnawy Engy , El-Masry Thanaa A. , Elseady Walaa S. , Saleh Asmaa , Alotaibi Khalid N. , El-Sherbeni Suzy A.
Published: November 2021

Lung diseases such as asthma, chronic obstructive pulmonary diseases, and pneumonia are causing many global health problems. The COVID-19 pandemic has directed the scientific community’s attention toward performing more research to explore novel therapeutic drugs for pulmonary diseases. Herein, gas chromatography coupled with mass spectrometry tentatively identified 44 compounds in frankincense ethanol extract (FEE). We investigated the antibacterial and antibiofilm effects of FEE against Pseudomonas aeruginosa bacteria, isolated from patients with respiratory infections. In addition, its in vitro immunomodulatory activity was explored by the detection of the gene expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), nitric oxide synthase (iNOS), cycloxygenase-2 (COX-2), and nuclear factor kappa-B (NF-κB) in lipopolysaccharide (LPS)-induced peripheral blood mononuclear cells (PBMC). In addition, its anticancer activity against the A549 lung cancer cell line and human skin fibroblast (HSF) normal cell line was studied. Moreover, the in vivo lung protective potential of FEE was explored histologically and immunohistochemically in mice using a benzo(a)pyrene induced lung damage model. FEE exhibited antibacterial and antibiofilm activities besides the significant inhibition of gene expression of TNFα, IL-6, and NF-κB. FEE also exerted a cytotoxic effect against A549 cell line. Histological and immunohistochemical investigations with morphometric analysis of the mean area percentage and color intensity of positive TNF-α, COX-2, and NF-κB and Bcl-2 reactions revealed the lung protective activity of FEE. This study outlined the promising therapeutic activity of oleoresin obtained from B. dalzielii in the treatment of different pulmonary diseases.
Clinical Features and Molecular Characteristics of Methicillin-Susceptible Staphylococcus aureus Ocular Infection in Taiwan
Authors: Chen Yueh-Ling , Kang Eugene Yu-Chuan , Yeh Lung-Kun , Ma David H. K. , Tan Hsin-Yuan , Chen Hung-Chi , Hung Kuo-Hsuan , Huang Yhu-Chering , Hsiao Ching-Hsi
Published: November 2021

This study analyzed the clinical features and molecular characteristics of methicillin-susceptible Staphylococcus aureus (MSSA) ocular infections in Taiwan and compared them between community-associated (CA) and health-care-associated (HA) infections. We collected S. aureus ocular isolates from patients at Chang Gung Memorial Hospital between 2010 and 2017. The infections were classified as CA or HA using epidemiological criteria, and the isolates were molecularly characterized using pulsed-field gel electrophoresis, multilocus sequence typing, and Panton-Valentine leukocidin (PVL) gene detection. Antibiotic susceptibility was evaluated using disk diffusion and an E test. A total of 104 MSSA ocular isolates were identified; 46 (44.2%) were CA-MSSA and 58 (55.8%) were HA-MSSA. Compared with HA-MSSA strains, CA-MSSA strains caused a significantly higher rate of keratitis, but a lower rate of conjunctivitis. We identified 14 pulsotypes. ST 7/pulsotype BA was frequently identified in both CA-MSSA (28.3%) and HA-MSSA (37.9%) cases. PVL genes were identified in seven isolates (6.7%). Both CA-MSSA and HA-MSSA isolates were highly susceptible to vancomycin, teicoplanin, tigecycline, sulfamethoxazole–trimethoprim, and fluoroquinolones. The most common ocular manifestations were keratitis and conjunctivitis for CA-MSSA and HA-MSSA, respectively. The MSSA ocular isolates had diverse molecular characteristics; no specific genotype differentiated CA-MSSA from HA-MSSA. Both strains exhibited similar antibiotic susceptibility.
Iron Overload, Oxidative Stress, and Ferroptosis in the Failing Heart and Liver
Authors: Mancardi Daniele , Mezzanotte Mariarosa , Arrigo Elisa , Barinotti Alice , Roetto Antonella
Published: November 2021

Iron accumulation is a key mediator of several cytotoxic mechanisms leading to the impairment of redox homeostasis and cellular death. Iron overload is often associated with haematological diseases which require regular blood transfusion/phlebotomy, and it represents a common complication in thalassaemic patients. Major damages predominantly occur in the liver and the heart, leading to a specific form of cell death recently named ferroptosis. Different from apoptosis, necrosis, and autophagy, ferroptosis is strictly dependent on iron and reactive oxygen species, with a dysregulation of mitochondrial structure/function. Susceptibility to ferroptosis is dependent on intracellular antioxidant capacity and varies according to the different cell types. Chemotherapy-induced cardiotoxicity has been proven to be mediated predominantly by iron accumulation and ferroptosis, whereas there is evidence about the role of ferritin in protecting cardiomyocytes from ferroptosis and consequent heart failure. Another paradigmatic organ for transfusion-associated complication due to iron overload is the liver, in which the role of ferroptosis is yet to be elucidated. Some studies report a role of ferroptosis in the initiation of hepatic inflammation processes while others provide evidence about an involvement in several pathologies including immune-related hepatitis and acute liver failure. In this manuscript, we aim to review the literature to address putative common features between the response to ferroptosis in the heart and liver. A better comprehension of (dys)similarities is pivotal for the development of future therapeutic strategies that can be designed to specifically target this type of cell death in an attempt to minimize iron-overload effects in specific organs.
Effects of Melatonin Supplementation during Pregnancy on Reproductive Performance, Maternal–Placental–Fetal Redox Status, and Placental Mitochondrial Function in a Sow Model
Authors: Peng Xie , Cai Xuelin , Li Jian , Huang Yingyan , Liu Hao , He Jiaqi , Fang Zhengfeng , Feng Bin , Tang Jiayong , Lin Yan , Jiang Xuemei , Hu Liang , Xu Shengyu , Zhuo Yong , Che Lianqiang , Wu De
Published: November 2021

Melatonin (MT) is a bio-antioxidant that has been widely used to prevent pregnancy complications, such as pre-eclampsia and IUGR during gestation. This experiment evaluated the impacts of dietary MT supplementation during pregnancy on reproductive performance, maternal–placental–fetal redox status, placental inflammatory response, and mitochondrial function, and sought a possible underlying mechanism in the placenta. Sixteen fifth parity sows were divided into two groups and fed each day of the gestation period either a control diet or a diet that was the same but for 36 mg of MT. The results showed that dietary supplementation with MT increased placental weight, while the percentage of piglets born with weight < 900 g decreased. Meanwhile, serum and placental MT levels, maternal–placental–fetal redox status, and placental inflammatory response were increased by MT. In addition, dietary MT markedly increased the mRNA levels of nutrient transporters and antioxidant-related genes involved in the Nrf2/ARE pathway in the placenta. Furthermore, dietary MT significantly increased ATP and NAD+ levels, relative mtDNA content, and the protein expression of Sirt1 in the placenta. These results suggested that MT supplementation during gestation could improve maternal–placental–fetal redox status and reproductive performance by ameliorating placental antioxidant status, inflammatory response, and mitochondrial dysfunction.
Profile of Phenolic Compounds of Prunus armeniaca L. Leaf Extract Determined by LC-ESI-QTOF-MS/MS and Their Antioxidant, Anti-Diabetic, Anti-Cholinesterase, and Anti-Inflammatory Potency
Authors: Wojdyło Aneta , Nowicka Paulina
Published: November 2021

In view of the limited information available in the literature concerning leaves as by-products of Prunus armeniaca cultivation, the aim of this work was to identify and characterize their principal polyphenolic constituents by LC-ESI-QTOF-MS/MS and screening in vitro biological potency as antioxidant capacity (ABTS, online ABTS, FRAP, ORAC), antidiabetic (α-amylase, α-glucosidase), anti-obesity (pancreatic lipase), anti-cholinesterase (AChE and BChE), and anti-inflammatory (COX-1 and COX-2) inhibitory activity. Comparison of different polyphenolic extracts of P. armeniaca cultivar leaves according to their quantitative composition revealed them to be exceptional sources of hydroxycinnamic acids, and to a lesser extent as sources of flavonols. Polyphenol-rich apricot leaf extract (PrALe) showed the most effective anti-obesity action through inhibition of pancreatic lipase, COX-1 and antioxidant capacity, especially the oxygen radical absorbance capacity, which was particularly correlated with polyphenolic compounds. Online ABTS radical UPLC-PDA-PDA analysis clearly demonstrated that the three predominant compounds of PrALe are quercetin-3-O-rutinoside > 5-O- and 3-O-caffeoylquinic acid, which basically contribute to antioxidant potential. These results assist in the evaluation of plant sources of potential new raw materials for application in different commercial sectors, especially for food, cosmetics and pharmaceuticals production.
Celastrol and Melatonin Modify SIRT1, SIRT6 and SIRT7 Gene Expression and Improve the Response of Human Granulosa-Lutein Cells to Oxidative Stress
Authors: Martín-Ramírez Rita , González-Fernández Rebeca , Hernández Jairo , Martín-Vasallo Pablo , Palumbo Angela , Ávila Julio
Published: November 2021

An excess of oxidative stress (OS) may affect several physiological processes fundamental to reproduction. SIRT1, SIRT6 and SIRT7 are involved in protection stress systems caused by OS, and they can be activated by antioxidants such as celastrol or melatonin. In this study, we evaluate SIRT1, SIRT6 and SIRT7 gene expression in cultured human granulosa-lutein (hGL) cells in response to OS inductors (glucose or peroxynitrite) and/or antioxidants. Our results show that celastrol and melatonin improve cell survival in the presence and absence of OS inductors. In addition, melatonin induced SIRT1, SIRT6 and SIRT7 gene expression while celastrol only induced SIRT7 gene expression. This response was not altered by the addition of OS inductors. Our previous data for cultured hGL cells showed a dual role of celastrol as a free radical scavenger and as a protective agent by regulating gene expression. This study shows a direct effect of celastrol on SIRT7 gene expression. Melatonin may protect from OS in a receptor-mediated manner rather than as a scavenger. In conclusion, our results show increased hGL cells survival with melatonin or celastrol treatment under OS conditions, probably through the regulation of nuclear sirtuins’ gene expression.
Reactive Oxygen Species as the Brainbox in Malaria Treatment
Authors: Egwu Chinedu Ogbonnia , Augereau Jean-Michel , Reybier Karine , Benoit-Vical Françoise
Published: November 2021

Several measures are in place to combat the worldwide spread of malaria, especially in regions of high endemicity. In part, most common antimalarials, such as quinolines and artemisinin and its derivatives, deploy an ROS-mediated approach to kill malaria parasites. Although some antimalarials may share similar targets and mechanisms of action, varying levels of reactive oxygen species (ROS) generation may account for their varying pharmacological activities. Regardless of the numerous approaches employed currently and in development to treat malaria, concerningly, there has been increasing development of resistance by Plasmodium falciparum, which can be connected to the ability of the parasites to manage the oxidative stress from ROS produced under steady or treatment states. ROS generation has remained the mainstay in enforcing the antiparasitic activity of most conventional antimalarials. However, a combination of conventional drugs with ROS-generating ability and newer drugs that exploit vital metabolic pathways, such antioxidant machinery, could be the way forward in effective malaria control.
Sodium Selenite Enhances Antibiotics Sensitivity of Pseudomonas aeruginosa and Deceases Its Pathogenicity by Inducing Oxidative Stress and Inhibiting Quorum Sensing System
Authors: Kong Weina , Tian Qianqian , Yang Qiaoli , Liu Yu , Wang Gongting , Cao Yanjun , Wang Liping , Xia Sizhe , Sun Yanmei , Zhao Cheng , Wang Shiwei
Published: November 2021

Pseudomonas aeruginosa, a Gram-negative opportunistic pathogen, is commonly found in clinical settings and immuno-compromised patients. It is difficult to be eradicated due to its strong antibiotic resistance, and novel inactivation strategies have yet to be developed. Selenium is an essential microelement for humans and has been widely used in dietary supplement and chemoprevention therapy. In this study, the physiological and biochemical effects of sodium selenite on P. aeruginosa PAO1 were investigated. The results showed that 0~5 mM sodium selenite did not impact the growth of PAO1, but increased the lethality rate of PAO1 with antibiotics or H2O2 treatment and the antibiotics susceptibility both in planktonic and biofilm states. In addition, sodium selenite significantly reduced the expression of quorum sensing genes and inhibited various virulence factors of this bacterium, including pyocyanin production, bacterial motilities, and the type III secretion system. Further investigation found that the content of ROS in cells was significantly increased and the expression levels of most genes involved in oxidative stress were up-regulated, which indicated that sodium selenite induced oxidative stress. The RNA-seq result confirmed the phenotypes of virulence attenuation and the expression of quorum sensing and antioxidant-related genes. The assays of Chinese cabbage and Drosophila melanogaster infection models showed that the combination of sodium selenite and antibiotics significantly alleviated the infection of PAO1. In summary, the results revealed that sodium selenite induced oxidative stress and inhibited the quorum sensing system of P. aeruginosa, which in turn enhanced the antibiotic susceptibility and decreased the pathogenicity of this bacterium. These findings suggest that sodium selenite may be used as an effective strategy for adjunct treatment of the infections caused by P. aeruginosa.
Radioprotective Role of Vitamins C and E against the Gamma Ray-Induced Damage to the Chemical Structure of Bovine Serum Albumin
Authors: Zarei Hajar , Bahreinipour Mostean , Sefidbakht Yahya , Rezaei Shokouh , Gheisari Rouhollah , Ardestani Susan Kabudanian , Uskoković Vuk , Watabe Hiroshi
Published: November 2021

Radioprotective effects of vitamin C and vitamin E as a water-soluble and a lipid-soluble agent, respectively, were investigated at the molecular level during the imposition of gamma radiation-induced structural changes to bovine serum albumin (BSA) at the therapeutic dose of 3 Gy. Secondary and tertiary structural changes of control and irradiated BSA samples were investigated using circular dichroism and fluorescence spectroscopy. The preirradiation tests showed nonspecific and reversible binding of vitamins C and E to BSA. Secondary and tertiary structures of irradiated BSA considerably changed in the absence of the vitamins. Upon irradiation, α-helices of BSA transitioned to beta motifs and random coils, and the fluorescence emission intensity decreased relative to nonirradiated BSA. In the presence of the vitamins C or E, however, the irradiated BSA was protected from these structural changes caused by reactive oxygen species (ROS). The two vitamins exhibited different patterns of attachment to the protein surface, as inspected by blind docking, and their mechanisms of protection were different. The hydrophilicity of vitamin C resulted in the predominant scavenging of ROS in the solvent, whereas hydrophobic vitamin E localized on the nonpolar patches of the BSA surface, where it did not only form a barrier for diffusing ROS but also encountered them as an antioxidant and neutralized them thanks to the moderate BSA binding constant. Very low concentrations of vitamins C or E (0.005 mg/mL) appear to be sufficient to prevent the oxidative damage of BSA.
Antioxidants in Animal Nutrition
Authors: Corino Carlo , Rossi Raffaella
Published: November 2021

Oxidative stress is an imbalance between the production of free radicals and their neutralization by the antioxidants’ defenses [...]
A Cardioplegic Solution with an Understanding of a Cardiochannelopathy
Authors: Ji Min Jeong , Hong Jeong Hee
Published: November 2021

Cardiac surgeries have been improved by accompanying developing cardioplegia solutions. However, the cardioplegia application presents an ongoing challenge with a view of a sufficiently restored cardiac function. In this review, we focus on the cardioplegia-induced mechanism and summarize the findings of studies undertaken to improve cardioprotective strategies. Currently, and somewhat surprisingly, relatively little is known about cardiac electrolyte regulation through channel physiology. We hope that an improved understanding of the electrolyte transport through ion channels/transporters and modulations of water channel aquaporins will provide an insight into cardiac channel physiology and a channel-based cardiac pathology of a cardiochannelopathy.
Unsupervised Anomalous Sound Detection for Machine Condition Monitoring Using Classification-Based Methods
Authors: Wang Yaoguang , Zheng Yaohao , Zhang Yunxiang , Xie Yongsheng , Xu Sen , Hu Ying , He Liang
Published: November 2021

The task of unsupervised anomalous sound detection (ASD) is challenging for detecting anomalous sounds from a large audio database without any annotated anomalous training data. Many unsupervised methods were proposed, but previous works have confirmed that the classification-based models far exceeds the unsupervised models in ASD. In this paper, we adopt two classification-based anomaly detection models: (1) Outlier classifier is to distinguish anomalous sounds or outliers from the normal; (2) ID classifier identifies anomalies using both the confidence of classification and the similarity of hidden embeddings. We conduct experiments in task 2 of DCASE 2020 challenge, and our ensemble method achieves an averaged area under the curve (AUC) of 95.82% and averaged partial AUC (pAUC) of 92.32%, which outperforms the state-of-the-art models.
Advance on the Capitalization of Grape Peels By-Product in Common Wheat Pasta
Authors: Ungureanu-Iuga Mădălina , Mironeasa Silvia
Published: November 2021

Capitalization of winery by-products has received high interest among scientists, producers and consumers concerned with healthy diet and environment protection. Grape peels are rich in fiber and polyphenols and can be used as ingredients in pasta matrix in order to increase the nutritional and functional value of such a staple food. The aim of this paper was to investigate the effects of grape peel flour added in various amounts (1–6%) to common wheat pasta dough viscoelasticity and texture and on pasta chemical composition, color, cooking behavior and texture, revealing at the same time the relations between characteristics. Grape peel flour induced the increase of the elastic (G′) and viscous (G″) moduli, dough hardness, springiness, cohesiveness, pasta crude ash, crude fat, crude fiber, total polyphenols and resistant starch contents, pasta water absorption, cooking loss and breaking force as the addition level was higher and compared to the control. On the other hand, dough resilience, pasta luminosity, chewiness and firmness decreased as the amount of grape peel flour raised. Significant correlations (p < 0.05) were obtained between the chemical composition and color parameters, while crude fiber, protein and fat were correlated with dough and pasta texture, total polyphenols with resistant starch content, cooking loss with crude fiber and dough textural parameters. The obtained results underlined the opportunity to use a valuable byproduct such as grape peels in novel pasta formulations, being helpful for processors to extend the product variety and to optimize the processes in order to better satisfy consumer’s demand for functional foods.
sST2 Predicts Short Term Therapy Success in Patients with Therapy Resistant Hypertension after Renal Sympathetic Denervation
Authors: Topf Albert , Paar Vera , Grueninger Janine , Wernly Bernhard , Kopp Kristen , Weber Thomas , Schernthaner Christiana , Mirna Moritz , Gharibeh Sarah , Larbig Robert , Pistulli Rudin , Hoppe Uta , Lichtenauer Michael , Motloch Lukas , Brandt Mathias
Published: November 2021

Background: Renal sympathetic denervation (RSD) has provided promising data in its ability to treat therapy resistant arterial hypertension. The effect of RSD on sST-2, a promising biomarker for risk stratification in cardiovascular diseases, has so far not been systematically studied. Methods: We evaluated serum levels of sST-2 and clinical parameter including left ventricular mass (LVM) in 54 patients with resistant hypertension (RH) undergoing bilateral RSD at baseline as well as at one and/or three months. Results: After RSD, mean office blood pressure showed a significant decrease after one month (p < 0.001). On echocardiography a reduction of LVM was observed at three months (p < 0.01). This was accompanied by a significant decrease of sST-2 levels at three months (sST-2 baseline: 6310.1 ± 3246.0 pg/mL vs. sST-2 three months: 4703.8 ± 1585.9 pg/mL, p = 0.048). Furthermore, baseline sST-2 levels were positively correlated with systolic blood pressure at one month (r = 0.514, p < 0.01) but not three months, indicating a potential predictive value of sST-2 for early intervention success. Conclusion: In patients with RH, RSD is associated with a significant decrease of sST-2 levels after three months, indicating sST-2 to be involved in remodeling processes after RSD. Furthermore, lower sST-2 levels at baseline might be a potential predictor of early intervention success of RSD.
MiA-CODER: A Multi-Intelligent Agent-Enabled Reinforcement Learning for Accurate Coverage Hole Detection and Recovery in Unequal Cluster-Tree-Based QoSensing WSN
Authors: Philco Luis Orlando , Marrone Luis , Estupiñan Emily
Published: November 2021

Coverage is an important factor for the effective transmission of data in the wireless sensor networks. Normally, the formation of coverage holes in the network deprives its performance and reduces the lifetime of the network. In this paper, a multi-intelligent agent enabled reinforcement learning-based coverage hole detection and recovery (MiA-CODER) is proposed in order to overcome the existing challenges related to coverage of the network. Initially, the formation of coverage holes is prevented by optimizing the energy consumption in the network. This is performed by constructing the unequal Sierpinski cluster-tree topology (USCT) and the cluster head is selected by implementing multi-objective black widow optimization (MoBWo) to facilitate the effective transmission of data. Further, the energy consumption of the nodes is minimized by performing dynamic sleep scheduling in which Tsallis entropy enabled Bayesian probability (TE2BP) is implemented to switch the nodes between active and sleep mode. Then, the coverage hole detection and repair are carried out in which the detection of coverage holes if any, both inside the cluster and between the clusters, is completed by using the virtual sector-based hole detection (ViSHD) protocol. Once the detection is over, the BS starts the hole repair process by using a multi-agent SARSA algorithm which selects the optimal mobile node and replaces it to cover the hole. By doing so, the coverage of the network is enhanced and better QoSensing is achieved. The proposed approach is simulated in NS 3.26 and evaluated in terms of coverage rate, number of dead nodes, average energy consumption and throughput.
A Novel Method for Estimating the Dosage of Cold Atmospheric Plasmas in Plasma Medical Applications
Authors: Li Jing , Zhao Lu-Xiang , He Tao , Dong Wei-Wu , Yuan Yue , Zhao Xiang , Chen Xin-Yi , Zhang Na , Zou Zhi-Fan , Zhang Yu , Li He-Ping
Published: November 2021

Cold atmospheric plasmas (CAPs) used in plasma medicine have shown great potential in various aspects including wound healing, dermatology, cancer therapy, etc. It is one of the important issues to determine the plasma dosage in plasma medicine because it dominates the specific plasma treatment results. However, the multi-process interactions between CAPs and biological materials make it rather challenging to give an accurate and versatile definition for plasma dosage. In this study, the ratio of the discharge energy to the number of the treated in vitro kidney cells (mJ/cell) was employed as the unit of the plasma dosage. Additionally, inspired by basic knowledge of pharmacy, the median lethal dose (LD50) was employed to help estimate the plasma dosage. The experimental results show that the value of LD50 using the newly designed CAP Bio-Med Platform for the kidney cells is 34.67 mJ/cell. This biology-based method has the advantages of easy operation, independence of specific CAP sources, and also independence of complex interactions between CAPs and the treated biological targets, and consequently, may provide a new direction to quantitatively define the plasma dosage in various plasma medical applications.
Gastrointestinal Disease Classification in Endoscopic Images Using Attention-Guided Convolutional Neural Networks
Authors: Lonseko Zenebe Markos , Adjei Prince Ebenezer , Du Wenju , Luo Chengsi , Hu Dingcan , Zhu Linlin , Gan Tao , Rao Nini
Published: November 2021

Gastrointestinal (GI) diseases constitute a leading problem in the human digestive system. Consequently, several studies have explored automatic classification of GI diseases as a means of minimizing the burden on clinicians and improving patient outcomes, for both diagnostic and treatment purposes. The challenge in using deep learning-based (DL) approaches, specifically a convolutional neural network (CNN), is that spatial information is not fully utilized due to the inherent mechanism of CNNs. This paper proposes the application of spatial factors in improving classification performance. Specifically, we propose a deep CNN-based spatial attention mechanism for the classification of GI diseases, implemented with encoder–decoder layers. To overcome the data imbalance problem, we adapt data-augmentation techniques. A total of 12,147 multi-sited, multi-diseased GI images, drawn from publicly available and private sources, were used to validate the proposed approach. Furthermore, a five-fold cross-validation approach was adopted to minimize inconsistencies in intra- and inter-class variability and to ensure that results were robustly assessed. Our results, compared with other state-of-the-art models in terms of mean accuracy (ResNet50 = 90.28, GoogLeNet = 91.38, DenseNets = 91.60, and baseline = 92.84), demonstrated better outcomes (Precision = 92.8, Recall = 92.7, F1-score = 92.8, and Accuracy = 93.19). We also implemented t-distributed stochastic neighbor embedding (t–SNE) and confusion matrix analysis techniques for better visualization and performance validation. Overall, the results showed that the attention mechanism improved the automatic classification of multi-sited GI disease images. We validated clinical tests based on the proposed method by overcoming previous limitations, with the goal of improving automatic classification accuracy in future work.
Physico-Mechanical Properties of Particleboards Produced from Macadamia Nutshell and Gum Arabic
Authors: Mirindi Derrick , Onchiri Richard O. , Thuo Joseph
Published: November 2021

Due to the rising prices and high demand for panels and the fact that formaldehyde—a known carcinogen—is used to manufacture conventional particleboard, this study investigated the suitability of particleboard formed from ground macadamia nutshells mixed with 50%, 40%, 30%, and 20% of gum Arabic and determined its physical and mechanical properties. The specific gravity, the bulk density, the x-ray fluorescence, and the scanning electron microscopy analysis of the two materials were analyzed. After production, the particleboards were cured for 56 days in an acclimatized room; then, the physical and mechanical properties were evaluated. Particleboards mixed with 50% gum Arabic and 50% macadamia nutshell showed good results in terms of the lowest average values of water absorption (9.42%) and thickness swelling (6.22%) after 24 h of immersion in distilled water as well as the highest density (1219.20 kg/m3), modulus of rupture (12.21 MPa), modulus of elasticity (1.81 GPa), internal bond strength (1.25 MPa), and compressive strength (22.54 MPa). According to ANSI/A208.1-1999, the particleboards produced met the standard for general-purpose boards except for water absorption (WA) and thickness swelling (TS) characteristics, which were above the maximum of 8% and 3%, respectively.
Evaluations of All-in-One, Polycarboxylate-Based Superplasticizer with Viscosity Modifying Agents for the Application of Normal-Strength, High-Fluidity Concrete
Authors: Kong Tae-Woong , Yang Hyun-Min , Lee Han-Seung , Yoon Chang-Bok
Published: November 2021

High fluidity concrete exhibits an excellent self-compacting property. However, the application of typical high-fluidity concrete is limited in the normal strength range (18~35 MPa) due to the large amount of binder. Therefore, it is important to solve these problems by adding a viscosity modifying agent (VMA) with a superplasticizer (PCE), which helps to improve the fluidity of the concrete. In addition, the rheology and stability of the concrete with VMA can be improved by preventing bleeding and segregation issues. Current studies focused on the physical phenomena of concrete such as the fluidity, rheological properties, and compressive strength of normal-strength, high-fluidity concrete (NSHFC) with different types of a polycarboxylate-based superplasticizer (NPCE). The obtained results suggested that the combinations of all-in-one polycarboxylate-based superplasticizers (NPCE) did not cause any cohesion or sedimentation even stored for a long time. The combination of three types of VMA showed the best fluidity (initial slump flow of 595~630 mm) without any segregation and bleeding, and the compressive strength at 28 days was also found to be the highest: 34–37 MPa. From these results, the combination of PCE (2.0%) + HPMC (0.3%) + WG (0.1%) + ST (0.1%) showed an 18% higher plastic viscosity and -4.4% lower yield stress than Plain.
Feasible Trajectories Generation for Autonomous Driving Vehicles
Authors: Vu Trieu Minh , Moezzi Reza , Cyrus Jindrich , Hlava Jaroslav , Petru Michal
Published: November 2021

This study presents smooth and fast feasible trajectory generation for autonomous driving vehicles subject to the vehicle physical constraints on the vehicle power, speed, acceleration as well as the hard limitations of the vehicle steering angle and the steering angular speed. This is due to the fact the vehicle speed and the vehicle steering angle are always in a strict relationship for safety purposes, depending on the real vehicle driving constraints, the environmental conditions, and the surrounding obstacles. Three different methods of the position quintic polynomial, speed quartic polynomial, and symmetric polynomial function for generating the vehicle trajectories are presented and illustrated with simulations. The optimal trajectory is selected according to three criteria: Smoother curve, smaller tracking error, and shorter distance. The outcomes of this paper can be used for generating online trajectories for autonomous driving vehicles and auto-parking systems.
Physical and Mathematical Modeling of the Interaction of Water Droplets and High-Speed Gas Flow
Authors: Minko Aleksandr , Guskov Oleg , Arefyev Konstantin , Saveliev Andrey
Published: November 2021

Present work is devoted to physical and mathematical modeling of the secondary disintegration of a liquid jet and gas-dynamic breakup of droplets in high-speed air flows. In this work the analysis of the experiments of water droplet breakup in the supersonic flow with Mach numbers up to M = 3 was carried out. The influence of shock wave presence in the flow on the intensity of droplets gas-dynamic breakup is shown. A developed empirical model is presented. It allows to predict the distribution of droplet diameters and velocities depending on the gas flow conditions, as well as the physical properties of the liquid. The effect of the Weber and Reynolds numbers on the rate of droplets gas-dynamic breakup at various Mach numbers is shown. The obtained data can be useful in the development of mathematical models for the numerical simulation of two-phase flows in the combined Lagrange-Euler formulation.