bluuebunny/crossref_metadata_2025 huggingface dataset:
An Experimental Study into the Behaviour of Tube and Fitting Scaffold Structures under Cyclic Side and Vertical Loads
Authors: Abdel-Jaber Ma’en , Abdel-Jaber Mu’tasim S. , Beale Robert G.
Published: December 2021

The United Kingdom and European codes for the analysis and design of tubular scaffold structures assume that the scaffolds are subjected primarily to vertical loads and to horizontal loads at right-angles to the scaffold. The effects of dynamic loading caused by large winds tend to be ignored and the code analyses often only require static loading on the structures to be considered. To investigate side loads, a scaffold frame built according to the UK standard was made and inserted into a testing rig. Five different load combinations were made to determine the behaviour of the scaffold under different side loads, which were varied cyclically to simulate different wind loads, especially when vertical loads were also applied. The results showed that cyclical loads affected scaffold behaviour, especially when the bases of the scaffold standards were not tied to the base at the bottom of the test rig. Changes should be made to the UK and European codes BS EN 74.1, BS 5975 and BS EN 128 11-1 for the design of scaffold structures to increase safety.
Study of Alloy Hot Flow and Hardening Behavior Using a New Correction Method for Hot Uniaxial Tests
Authors: Qu Shuguang , Peng Heli , He Zhubin , Zheng Kailun , Zheng Jinghua
Published: December 2021

The precise characterisation of hot flow behavior of titanium alloys is of vital importance for practical hot forming processes. To precisely determine the hot flow behavior of titanium alloys under the forming conditions, Gleeble hot tensile tests are usually performed to simulate the forming processes by accurately controlling the deformation temperatures and strain rates under designed conditions. However, there exists a non-uniform temperature distribution during the Gleeble tests, which leads to inaccuracies in the determined hot flow behavior. To overcome such an issue, this paper proposed a new strain-based correction method for Gleeble hot tensile tests, enabling the mitigation of the non-uniform temperature-induced stress-strain curve inaccuracies. The non-uniform temperature zones have been successfully excluded in the calculation of the true strain levels. A series of hot uniaxial tensile tests of TA32 at temperatures, ranging from 750 °C to 900 °C, and strain rates, 0.01/s~1/s, were carried out. The obtained stress-strain correlations for a large gauge zone were characterized using the new correction method, which was further used to evaluate the hardening behavior of titanium alloys. The results have shown that the ductility, strain hardening component (i.e., n), strain rate hardening component (i.e., m) and uniform strain value (i.e., εu) are over-estimated, compared to conventional method. Higher strain rates and lower temperature leads to enhanced hardening behavior. This research provides an alternative correction method and may achieve more accurate stress-strain curves for better guidance of the hot forming process for titanium alloys.
Genome-Scale Metabolic Modeling Enables In-Depth Understanding of Big Data
Authors: Passi Anurag , Tibocha-Bonilla Juan D. , Kumar Manish , Tec-Campos Diego , Zengler Karsten , Zuniga Cristal
Published: December 2021

Genome-scale metabolic models (GEMs) enable the mathematical simulation of the metabolism of archaea, bacteria, and eukaryotic organisms. GEMs quantitatively define a relationship between genotype and phenotype by contextualizing different types of Big Data (e.g., genomics, metabolomics, and transcriptomics). In this review, we analyze the available Big Data useful for metabolic modeling and compile the available GEM reconstruction tools that integrate Big Data. We also discuss recent applications in industry and research that include predicting phenotypes, elucidating metabolic pathways, producing industry-relevant chemicals, identifying drug targets, and generating knowledge to better understand host-associated diseases. In addition to the up-to-date review of GEMs currently available, we assessed a plethora of tools for developing new GEMs that include macromolecular expression and dynamic resolution. Finally, we provide a perspective in emerging areas, such as annotation, data managing, and machine learning, in which GEMs will play a key role in the further utilization of Big Data.
Metabolomic Profiles of Mouse Tissues Reveal an Interplay between Aging and Energy Metabolism
Authors: Zhou Qishun , Kerbl-Knapp Jakob , Zhang Fangrong , Korbelius Melanie , Kuentzel Katharina Barbara , Vujić Nemanja , Akhmetshina Alena , Hörl Gerd , Paar Margret , Steyrer Ernst , Kratky Dagmar , Madl Tobias
Published: December 2021

Energy metabolism, including alterations in energy intake and expenditure, is closely related to aging and longevity. Metabolomics studies have recently unraveled changes in metabolite composition in plasma and tissues during aging and have provided critical information to elucidate the molecular basis of the aging process. However, the metabolic changes in tissues responsible for food intake and lipid storage have remained unexplored. In this study, we aimed to investigate aging-related metabolic alterations in these tissues. To fill this gap, we employed NMR-based metabolomics in several tissues, including different parts of the intestine (duodenum, jejunum, ileum) and brown/white adipose tissues (BAT, WAT), of young (9–10 weeks) and old (96–104 weeks) wild-type (mixed genetic background of 129/J and C57BL/6) mice. We, further, included plasma and skeletal muscle of the same mice to verify previous results. Strikingly, we found that duodenum, jejunum, ileum, and WAT do not metabolically age. In contrast, plasma, skeletal muscle, and BAT show a strong metabolic aging phenotype. Overall, we provide first insights into the metabolic changes of tissues essential for nutrient uptake and lipid storage and have identified biomarkers for metabolites that could be further explored, to study the molecular mechanisms of aging.
Opto-Microfluidic Fabry-Perot Sensor with Extended Air Cavity and Enhanced Pressure Sensitivity
Authors: Zhang Pengfei , Wang Chao , Wan Liuwei , Zhang Qianqian , Gong Zidan , Qin Zixiong , Chan Chi Chiu
Published: December 2021

An opto-microfluidic static pressure sensor based on a fiber Fabry-Perot Interferometer (FPI) with extended air cavity for enhancing the measuring sensitivity is proposed. The FPI is constructed in a microfluidic channel by the combination of the fixed fiber-end reflection and floating liquid surface reflection faces. A change of the aquatic pressure will cause a drift of the liquid surface and the pressure can be measured by detecting the shift of the FPI spectrum. Sensitivity of the sensor structure can be enhanced significantly by extending the air region of the FPI. The structure is manufactured by using a common single-mode optical fiber, and a silica capillary with the inner wall coated with a hydrophobic film. A sample with 3500 μm air cavity length has demonstrated the pressure sensitivity of about 32.4 μm/kPa, and the temperature cross-sensitivity of about 0.33 kPa/K.
A Novel MEMS Capacitive Microphone with Semiconstrained Diaphragm Supported with Center and Peripheral Backplate Protrusions
Authors: Shubham Shubham , Seo Yoonho , Naderyan Vahid , Song Xin , Frank Anthony , Johnson Jeremy , da Silva Mark , Pedersen Michael
Published: December 2021

Audio applications such as mobile phones, hearing aids, true wireless stereo earphones, and Internet of Things devices demand small size, high performance, and reduced cost. Microelectromechanical system (MEMS) capacitive microphones fulfill these requirements with improved reliability and specifications related to sensitivity, signal-to-noise ratio (SNR), distortion, and dynamic range when compared to their electret condenser microphone counterparts. We present the design and modeling of a semiconstrained polysilicon diaphragm with flexible springs that are simply supported under bias voltage with a center and eight peripheral protrusions extending from the backplate. The flexible springs attached to the diaphragm reduce the residual film stress effect more effectively compared to constrained diaphragms. The center and peripheral protrusions from the backplate further increase the effective area, linearity, and sensitivity of the diaphragm when the diaphragm engages with these protrusions under an applied bias voltage. Finite element modeling approaches have been implemented to estimate deflection, compliance, and resonance. We report an 85% increase in the effective area of the diaphragm in this configuration with respect to a constrained diaphragm and a 48% increase with respect to a simply supported diaphragm without the center protrusion. Under the applied bias, the effective area further increases by an additional 15% as compared to the unbiased diaphragm effective area. A lumped element model has been also developed to predict the mechanical and electrical behavior of the microphone. With an applied bias, the microphone has a sensitivity of −38 dB (ref. 1 V/Pa at 1 kHz) and an SNR of 67 dBA measured in a 3.25 mm × 1.9 mm × 0.9 mm package including an analog ASIC.
Towards Repeatable, Scalable Graphene Integrated Micro-Nano Electromechanical Systems (MEMS/NEMS)
Authors: Cho Joon Hyong , Cayll David , Behera Dipankar , Cullinan Michael
Published: December 2021

The demand for graphene-based devices is rapidly growing but there are significant challenges for developing scalable and repeatable processes for the manufacturing of graphene devices. Basic research on understanding and controlling growth mechanisms have recently enabled various mass production approaches over the past decade. However, the integration of graphene with Micro-Nano Electromechanical Systems (MEMS/NEMS) has been especially challenging due to performance sensitivities of these systems to the production process. Therefore, ability to produce graphene-based devices on a large scale with high repeatability is still a major barrier to the commercialization of graphene. In this review article, we discuss the merits of integrating graphene into Micro-Nano Electromechanical Systems, current approaches for the mass production of graphene integrated devices, and propose solutions to overcome current manufacturing limits for the scalable and repeatable production of integrated graphene-based devices.
The Role of Soft Robotic Micromachines in the Future of Medical Devices and Personalized Medicine
Authors: Garcia Lourdes , Kerns Genevieve , O’Reilley Kaitlin , Okesanjo Omolola , Lozano Jacob , Narendran Jairaj , Broeking Conor , Ma Xiaoxiao , Thompson Hannah , Njapa Njeuha Preston , Sikligar Drashti , Brockstein Reed , Golecki Holly M.
Published: December 2021

Developments in medical device design result in advances in wearable technologies, minimally invasive surgical techniques, and patient-specific approaches to medicine. In this review, we analyze the trajectory of biomedical and engineering approaches to soft robotics for healthcare applications. We review current literature across spatial scales and biocompatibility, focusing on engineering done at the biotic-abiotic interface. From traditional techniques for robot design to advances in tunable material chemistry, we look broadly at the field for opportunities to advance healthcare solutions in the future. We present an extracellular matrix-based robotic actuator and propose how biomaterials and proteins may influence the future of medical device design.
Influence of Some Microchanges Generated by Different Processing Methods on Selected Tribological Characteristics
Authors: Nagîț Gheorghe , Slătineanu Laurențiu , Dodun Oana , Mihalache Andrei Marius , Rîpanu Marius Ionuț , Hriţuc Adelina
Published: December 2021

Different processing methods can change the physical–mechanical properties and the microgeometry of the surfaces made by such processes. In turn, such microchanges may affect the tribological characteristics of the surface layer. The purpose of this research was to study the tribological behavior of a test piece surfaces analyzing the changes on the values of the coefficient of friction and loss of mass that appear in time. The surfaces subjected to experimental research were previously obtained by turning, grinding, ball burnishing, and vibroburnishing. The experimental research was performed using a device adaptable to a universal lathe. Mathematical processing of the experimental results led to the establishment of power-type function empirical models that highlight the intensity of the influence exerted by the pressure and duration of the test on the values of the output parameters. It was found that the best results were obtained in the case of applying ball vibroburnishing as the final process.
High Sulfur in Primitive Arc Magmas, Its Origin and Implications
Authors: Zelenski Michael , Kamenetsky Vadim S. , Nekrylov Nikolai , Kontonikas-Charos Alkiviadis
Published: December 2021

Sulfur contents in 98.5% of melt inclusions (MI) from calc-alkaline subduction basalts do not exceed 4000 ppm, whereas experimentally established limits of sulfur solubility in basaltic melts with high fO2 (characteristic of subduction zones, e.g., QFM + 2) surpass 14,000 ppm. Here we show that primitive (Mg# 62-64) subduction melts may contain high sulfur, approaching the experimental limit of sulfur solubility. Up to 11,700 ppm S was measured in olivine-hosted MI from primitive arc basalt from the 1941 eruption of the Tolbachik volcano, Kamchatka. These MI often contain magmatic sulfide globules (occasionally enriched in Cu, Ni, and platinum-group elements) and anhydrite enclosed within a brown, oxidized glass. We conclude that the ubiquitous low sulfur contents in MI may originate either from insufficient availability of sulfur in the magma generation zone or early magma degassing prior to inclusion entrapment. Our findings extend the measured range of sulfur concentrations in primitive calc-alkaline basaltic melts and demonstrate that no fundamental limit of 4000 ppm S exists for relatively oxidized subduction basalts, where the maximum sulfur content may approach the solubility limit determined by crystallization of magmatic anhydrite.
Optimization of Flavonoid Extraction from Xanthoceras sorbifolia Bunge Flowers, and the Antioxidant and Antibacterial Capacity of the Extract
Authors: Zhang Henghui , Wang Xiaoli , He Dongliang , Zou Dongliang , Zhao Runzhu , Wang Huifang , Li Shuying , Xu Yongping , Abudureheman Buhailiqiemu
Published: December 2021

In the present work, the extraction process of total flavonoids (TFs) from X. sorbifolia flowers by ultrasound-assisted extraction was optimized under the response surface methodology (RSM) on the basis of single-factor experiments. The optimal extraction conditions were as follows: ethanol concentration of 80%, solid–liquid ratio of 1:37 (g/mL), temperature of 84 °C, and extraction time of 1 h. Under the optimized conditions, the extraction yield of the TFs was 3.956 ± 0.04%. The radical scavenging capacities of TFs against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) were much greater than that of rutin. The results of antibacterial experiments indicated that the TFs displayed strong inhibitory activities on E. coli, S. aureus and Bacillus subtilis. Therefore, X. sorbifolia flowers can be used as a novel source of natural flavonoids, and the TFs have potential applications as natural antioxidants or antibacterial agents in the food and pharmaceutical industries.
A Review of Recent Advances in Superhydrophobic Surfaces and Their Applications in Drag Reduction and Heat Transfer
Authors: Zhang Yu , Zhang Zhentao , Yang Junling , Yue Yunkai , Zhang Huafu
Published: December 2021

Inspired by the superhydrophobic properties of some plants and animals with special structures, such as self-cleaning, water repellent, and drag reduction, the research on the basic theory and practical applications of superhydrophobic surfaces is increasing. In this paper, the characteristics of superhydrophobic surfaces and the preparation methods of superhydrophobic surfaces are briefly reviewed. The mechanisms of drag reduction on superhydrophobic surfaces and the effects of parameters such as flow rate, fluid viscosity, wettability, and surface morphology on drag reduction are discussed, as well as the applications of superhydrophobic surfaces in boiling heat transfer and condensation heat transfer. Finally, the limitations of adapting superhydrophobic surfaces to industrial applications are discussed. The possibility of applying superhydrophobic surfaces to highly viscous fluids for heat transfer to reduce flow resistance and improve heat transfer efficiency is introduced as a topic for further research in the future.
Effect of Amorphous Crosslinker on Phase Behavior and Electro-Optic Response of Polymer-Stabilized Blue Phase Liquid Crystals
Authors: Lee Kyung Min , Tohgha Urice , Bunning Timothy J. , McConney Michael E. , Godman Nicholas P.
Published: December 2021

Blue phase liquid crystals (BPLCs) composed of double-twisted cholesteric helices are promising materials for use in next-generation displays, optical components, and photonics applications. However, BPLCs are only observed in a narrow temperature range of 0.5–3 °C and must be stabilized with a polymer network. Here, we report on controlling the phase behavior of BPLCs by varying the concentration of an amorphous crosslinker (pentaerythritol triacrylate (PETA)). LC mixtures without amorphous crosslinker display narrow phase transition temperatures from isotropic to the blue phase-II (BP-II), blue phase-I (BP-I), and cholesteric phases, but the addition of PETA stabilizes the BP-I phase. A PETA content above 3 wt% prevents the formation of the simple cubic BP-II phase and induces a direct transition from the isotropic to the BP-I phase. PETA widens the temperature window of BP-I from ~6.8 °C for BPLC without PETA to ~15 °C for BPLC with 4 wt% PETA. The BPLCs with 3 and 4 wt% PETA are stabilized using polymer networks via in situ photopolymerization. Polymer-stabilized BPLC with 3 wt% PETA showed switching between reflective to transparent states with response times of 400–500 μs when an AC field was applied, whereas the application of a DC field induced a large color change from green to red.
Green Synthesis of Nano Zinc Oxide/Nanohydroxyapatite Composites Using Date Palm Pits Extract and Eggshells: Adsorption and Photocatalytic Degradation of Methylene Blue
Authors: Elsayed Maha S. , Ahmed Inas A. , Bader Dina M. D. , Hassan Asaad F.
Published: December 2021

In this study, zinc oxide nanoparticles (ZnO) and nanohydroxyapatite (NHAP) were prepared in the presence of date palm pits extract (DPPE) and eggshells, respectively. Another four nanocomposites were prepared from ZnO and NHAP in different ratios (ZP13, ZP14, ZP15, and ZP16). DPPE and all nanomaterials were characterized using GC-MS, zeta potentials, particle size distributions, XRD, TEM, EDX, FTIR, and pHPZC. The characterization techniques confirmed the good distribution of ZnO nanoparticles on the surface of NHAP in the prepared composites. Particles were found to be in the size range of 42.3–66.1 nm. The DPPE analysis confirmed the presence of various natural chemical compounds which act as capping agents for nanoparticles. All the prepared samples were applied in the adsorption and photocatalytic degradation of methylene blue under different conditions. ZP14 exhibited the maximum adsorption capacity (596.1 mg/g) at pH 8, with 1.8 g/L as the adsorbent dosage, after 24 h of shaking time, and the static adsorption kinetic process followed a PSO kinetic model. The photocatalytic activity of ZP14 reached 91% after 100 min of illumination at a lower MB concentration (20 mg/L), at pH 8, using 1.5 g/L as the photocatalyst dosage, at 25 °C. The photocatalytic degradation of MB obeyed the Langmuir–Hinshelwood first-order kinetic model, and the photocatalyst reusability exhibited a slight loss in activity (~4%) after five cycles of application.
Effective Inhibition of Invasive Pulmonary Aspergillosis by Silver Nanoparticles Biosynthesized with Artemisia sieberi Leaf Extract
Authors: Ali Enas M. , Abdallah Basem M.
Published: December 2021

Aspergillus fumigatus is one of the most common fungal pathogens that can cause a diversity of diseases ranging from invasive pulmonary aspergillosis (IPA) and aspergilloma to allergic syndromes. In this study, we investigated the antifungal effect of silver nanoparticles biosynthesized with Artemisia sieberi leaf extract (AS-AgNPs) against A. fumigatus in vitro and in vivo. The biosynthesized AS-AgNPs were characterized by imaging (transmission electron microscopy (TEM)), UV−VIS spectroscopy, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The microdilution method showed the antifungal activity of AS-AgNPs against A. fumigatus, with an MIC of 128 µg/mL. AS-AgNPs significantly inhibited the growth of hyphae in all directions, as imaged by SEM. Additionally, TEM on biofilm revealed invaginations of the cell membrane, a change in the vacuolar system, and the presence of multilamellar bodies within vacuoles. Interestingly, AS-AgNPs displayed low cytotoxicity on the A549 human lung cell line in vitro. Treatment of an invasive pulmonary aspergillosis (IPA) mouse model with AS-AgNPs demonstrated the potency of AS-AgNPs to significantly reduce lung tissue damage and to suppress the elevated levels of pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-α), interleukin-1 (IL-1), and interleukin-17 (IL-17). The therapeutic potential of AS-AgNPs was found to be due to their direct action to suppress the fungal burden and gliotoxin production in the lungs. In addition, AS-AgNPs reduced the oxidative stress in the lungs by increasing the enzymatic activities of catalase (CAT) and superoxide dismutase (SOD). Thus, our data indicate the biosynthesized AS-AgNPs as a novel antifungal alternative treatment against aspergillosis.
3D Laser Nanoprinting of Optically Functionalized Structures with Effective-Refractive-Index Tailorable TiO2 Nanoparticle-Doped Photoresin
Authors: Song Shichao , Li Yijie , Yao Zhuofan , Li Jie , Li Xiangping , Cao Yaoyu
Published: December 2021

The advanced direct laser printing of functional devices with tunable effective index is a key research topic in numerous emerging fields, especially in micro-/nano-optics, nanophotonics, and electronics. Photosensitized nanocomposites, consisting of high-index materials (e.g., titanium dioxide, TiO2) embedded in polymer matrix, are emerging as attractive platforms for advanced additive manufacturing. Unfortunately, in the currently applied techniques, the preparation of optically functionalized structures based on these photosensitized nanocomposites is still hampered by many issues like hydrolysis reaction, high-temperature calcinations, and, especially, the complexity of experimental procedures. In this study, we demonstrate a feasible strategy for fabricating micro-/nanostructures with a flexibly manipulated effective refractive index by incorporating TiO2 nanoparticles in the matrix of acrylate resin, i.e., TiO2-based photosensitized nanocomposites. It was found that the effective refractive index of nanocomposite can be easily tuned by altering the concentration of titanium dioxide nanoparticles in the monomer matrix. For TiO2 nanoparticle concentrations up to 30 wt%, the refractive index can be increased over 11.3% (i.e., altering from 1.50 of pure monomer to 1.67 at 532 nm). Based on such a photosensitized nanocomposite, the grating structures defined by femtosecond laser nanoprinting can offer vivid colors, ranging from crimson to magenta, as observed in the dark-field images. The minimum printing width and printing resolution are estimated at around 70 nm and 225 nm, indicating that the proposed strategy may pave the way for the production of versatile, scalable, and functionalized opto-devices with controllable refractive indices.
Edge Doping Engineering of High-Performance Graphene Nanoribbon Molecular Spintronic Devices
Authors: Wan Haiqing , Xiao Xianbo , Ang Yee
Published: December 2021

We study the quantum transport properties of graphene nanoribbons (GNRs) with a different edge doping strategy using density functional theory combined with nonequilibrium Green’s function transport simulations. We show that boron and nitrogen edge doping on the electrodes region can substantially modify the electronic band structures and transport properties of the system. Remarkably, such an edge engineering strategy effectively transforms GNR into a molecular spintronic nanodevice with multiple exceptional transport properties, namely: (i) a dual spin filtering effect (SFE) with 100% filtering efficiency; (ii) a spin rectifier with a large rectification ratio (RR) of 1.9 ×106; and (iii) negative differential resistance with a peak-to-valley ratio (PVR) of 7.1 ×105. Our findings reveal a route towards the development of high-performance graphene spintronics technology using an electrodes edge engineering strategy.
Guillain-Barré Syndrome in the COVID-19 Pandemic
Authors: Tawakul Abdullah Ahmad , Al-Doboke Amal Waleed , Altayyar Shahad Ali , Alsulami Seham Abdulhafith , Alfahmi Ahlam Musallam , Nooh Raghad Turki
Published: December 2021

There have been several reported cases of severe acute respiratory syndrome (SARS-CoV-2) infection that were associated with an increased incidence of neurological manifestations, including Guillain–Barré syndrome (GBS). This review aims to present information on the reports of GBS associated with coronavirus disease 2019 (COVID-19) infection. Our review is retrospective work examining articles published from the 1 April 2020 to the 8 May 2021 in the English language. We used the diagnostic criteria and classification published by the National Institute of Neurological Disorders and Stroke and Brighton Collaboration. GBS is usually a postinfectious syndrome, but GBS in the COVID-19 pandemic also takes on a para-infectious profile. In the reports, the genetic factor has a role in developing GBS in some patients. In conclusion, the association between COVID-19 and GBS is not very clear. Still, one mechanism is strongly associated with COVID-19 and immune-mediated neurological complications, which is molecular mimicry between SARS-CoV-2 and human autoantigens.
Moringa oleifera Leaf Supplementation as a Glycemic Control Strategy in Subjects with Prediabetes
Authors: Gómez-Martínez Sonia , Díaz-Prieto Ligia E. , Vicente Castro Iván Vicente , Jurado César , Iturmendi Nerea , Martín-Ridaura Maria Carmen , Calle Nuria , Dueñas María , Picón María J. , Marcos Ascensión , Nova Esther
Published: December 2021

Moringa oleifera (MO) is a multipurpose plant with a high polyphenol content, which is being increasingly consumed to lessen the risk of chronic metabolic diseases such as Type 2 diabetes; however, scientific evidence from clinical trials is scarce. A double-blind, randomized, placebo-controlled, parallel group intervention study with MO leaves as a food supplement was conducted in subjects with prediabetes. They consumed six daily capsules of MO dry leaf powder (2400 mg/day) (MO, n = 31) or placebo (PLC, n = 34) over 12 weeks. Glycemia, appetite-controlling hormones and gut microbiota composition were studied. ANCOVA with the fixed factor “treatment” and the basal value as covariate was used to compare the change score between the groups. The results showed significant differences between groups in the rate of change of fasting blood glucose (FBG) and glycated hemoglobin (HbA1c), which showed opposite directions during the intervention, decreasing in MO and increasing in PLC. No different change scores were found between the groups in microbiota, hepatic and renal function markers or the appetite-controlling hormones measured. In conclusion, MO supplementation resulted in favorable changes in glycaemia markers compared to placebo in the subjects with prediabetes studied, suggesting that MO might act as a natural antihyperglycemic agent.
Association between Metabolic Syndrome Diagnosis and the Physical Activity—Sedentary Profile of Adolescents with Obesity: A Complementary Analysis of the Beta-JUDO Study
Authors: Julian Valérie , Ciba Iris , Olsson Roger , Dahlbom Marie , Furthner Dieter , Gomahr Julian , Maruszczak Katharina , Morwald Katharina , Pixner Thomas , Schneider Anna , Pereira Bruno , Duclos Martine , Weghuber Daniel , Thivel David , Bergsten Peter , Forslund Anders
Published: December 2021

Metabolic syndrome (MetS) is highly prevalent in children and adolescents with obesity and places them at an increased risk of cardiovascular-related diseases. However, the associations between objectively measured movement-related behaviors and MetS diagnosis remain unexplored in youths with obesity. The aim was to compare profiles of sedentary (SED) time (more sedentary, SED+ vs. less sedentary, SED−), moderate to vigorous physical activity (MVPA) time (more active, MVPA+ vs. less active, MVPA−) and combinations of behaviors (SED−/MVPA+, SED−/MVPA−, SED+/MVPA+, SED+/MVPA−) regarding the MetS diagnosis. One hundred and thirty-four adolescents with obesity (13.4 ± 2.2 years) underwent 24 h/7 day accelerometry, waist circumference (WC), blood pressure (BP), high-density lipoprotein-cholesterol (HDL-c), triglycerides (TG) and insulin-resistance (IR) assessments. Cumulative cardiometabolic risk was assessed by using (i) MetS status (usual dichotomic definition) and (ii) cardiometabolic risk z-score (MetScore, mean of standardized WC, BP, IR, TG and inverted HDL-c). SED− vs. SED+ and MVPA+ vs. MVPA− had lower MetS (p < 0.01 and p < 0.001) and MetScore (p < 0.001). SED−/MVPA+ had the lowest risk. While SED and MVPA times were lower in SED−/MVPA− vs. SED+/MVPA+ (p < 0.001), MetScore was lower in SED−/MVPA− independently of body mass index (BMI) (p < 0.05). MVPA, but not SED, time was independently associated with MetS diagnosis (p < 0.05). Both MVPA (p < 0.01) and SED times (p < 0.05) were associated with MetScore independently of each other. A higher MVPA and lower SED time are associated with lower cumulative cardiometabolic risk.
Association between Fruit Consumption and Lipid Profile among Children and Adolescents: A National Cross-Sectional Study in China
Authors: Liu Jieyu , Li Yanhui , Wang Xinxin , Gao Di , Chen Li , Chen Manman , Ma Tao , Ma Qi , Ma Ying , Zhang Yi , Jiang Jun , Zou Zhiyong , Wang Xijie , Dong Yanhui , Ma Jun
Published: December 2021

To investigate associations between fruit consumption and lipid profiles, and to further explore a satisfactory level of frequency and daily fruit intake for children and adolescents. A national sample of 14,755 children and adolescents aged 5–19 years from seven provinces in China were recruited. Fasting blood samples were collected to test the lipid profile. Information regarding fruit consumption and other characteristics was collected by questionnaires. Logistic regression models adjusting for confounding covariates were applied to calculate the odds ratio (OR) and 95% confidence interval (95% CI). Participants who consumed fruits for 6–7 days per week had lower risks of high triglycerides (OR: 0.66, 95% CI: 0.58–0.75), dyslipidemia (OR: 0.77, 95% CI: 0.68–0.86), and hyperlipidemia (OR: 0.72, 95% CI: 0.63–0.81), compared to fruit consumption of 0–2 days per week. Risks of high triglycerides, dyslipidemia and hyperlipidemia of those who consumed fruits for 0.75–1.5 servings each day also decreased, compared to the insufficient fruit intake. The combined effects of high frequency and moderate daily intake of fruit on lipid disorders did not change essentially. The associations were more evident in girls, younger children and those whose families had higher educational levels. Moderate fruit consumption was associated with lower odds of lipid disorders, predominantly in girls, younger participants, and those came from higher-educated families. These findings supported the health effect of moderate fruit intake frequently to improve the childhood lipid profiles.
Autonomic Nervous System Neuroanatomical Alterations Could Provoke and Maintain Gastrointestinal Dysbiosis in Autism Spectrum Disorder (ASD): A Novel Microbiome–Host Interaction Mechanistic Hypothesis
Authors: Beopoulos Athanasios , Gea Manuel , Fasano Alessio , Iris François
Published: December 2021

Dysbiosis secondary to environmental factors, including dietary patterns, antibiotics use, pollution exposure, and other lifestyle factors, has been associated to many non-infective chronic inflammatory diseases. Autism spectrum disorder (ASD) is related to maternal inflammation, although there is no conclusive evidence that affected individuals suffer from systemic low-grade inflammation as in many psychological and psychiatric diseases. However, neuro-inflammation and neuro–immune abnormalities are observed within ASD-affected individuals. Rebalancing human gut microbiota to treat disease has been widely investigated with inconclusive and contradictory findings. These observations strongly suggest that the forms of dysbiosis encountered in ASD-affected individuals could also originate from autonomic nervous system (ANS) functioning abnormalities, a common neuro–anatomical alteration underlying ASD. According to this hypothesis, overactivation of the sympathetic branch of the ANS, due to the fact of an ASD-specific parasympathetic activity deficit, induces deregulation of the gut–brain axis, attenuating intestinal immune and osmotic homeostasis. This sets-up a dysbiotic state, that gives rise to immune and osmotic dysregulation, maintaining dysbiosis in a vicious cycle. Here, we explore the mechanisms whereby ANS imbalances could lead to alterations in intestinal microbiome–host interactions that may contribute to the severity of ASD by maintaining the brain–gut axis pathways in a dysregulated state.
Excess Folic Acid Supplementation before and during Pregnancy and Lactation Alters Behaviors and Brain Gene Expression in Female Mouse Offspring
Authors: Yang Xingyue , Sun Wenyan , Wu Qian , Lin Hongyan , Lu Zhixing , Shen Xin , Chen Yongqi , Zhou Yan , Huang Li , Wu Feng , Liu Fei , Chu Dandan
Published: December 2021

Use of folic acid (FA) during early pregnancy protects against birth defects. However, excess FA has shown gender-specific neurodevelopmental toxicity. Previously, we fed the mice with 2.5 times the recommended amount of FA one week prior to mating and during the pregnancy and lactation periods, and detected the activated expression of Fos and related genes in the brains of weaning male offspring, as well as behavioral abnormalities in the adults. Here, we studied whether female offspring were affected by the same dosage of FA. An open field test, three-chamber social approach and social novelty test, an elevated plus-maze, rotarod test and the Morris water maze task were used to evaluate their behaviors. RNA sequencing was performed to identify differentially expressed genes in the brains. Quantitative real time-PCR (qRT-PCR) and Western blots were applied to verify the changes in gene expression. We found increased anxiety and impaired exploratory behavior, motor coordination and spatial memory in FA-exposed females. The brain transcriptome revealed 36 up-regulated and 79 down-regulated genes in their brains at weaning. The increase of Tlr1; Sult1a1; Tph2; Acacb; Etnppl; Angptl4 and Apold1, as well as a decrease of Ppara mRNA were confirmed by qRT-PCR. Among these genes; the mRNA levels of Etnppl; Angptl4andApold1 were increased in the both FA-exposed female and male brains. The elevation of Sult1a1 protein was confirmed by Western blots. Our data suggest that excess FA alteres brain gene expression and behaviors in female offspring, of which certain genes show apparent gender specificity.
Positive Association between High Protein Food Intake Frequency and Physical Performance and Higher-Level Functional Capacity in Daily Life
Authors: Kimura Mika , Moriyasu Ai , Makizako Hyuma
Published: December 2021

Nutritional factors, including low protein intake and poor dietary variety, affect age-associated impairment in physical performance resulting in physical frailty. This cross-sectional study investigated the association between intake frequency of major high protein foods and both physical performance and higher-level functional capacity using the food frequency score (FFS) and high protein food frequency score (PFFS) among community-dwelling older adults. The data of 1185 older adults categorized into quartiles based on FFS and PFFS were analyzed. After adjusting for covariates, FFS and PFFS were significantly associated with physical performance [FFS, usual gait speed (p for trend = 0.007); PFFS, usual gait speed (p for trend < 0.001), maximum gait speed (p for trend = 0.002), timed up and go (p for trend = 0.025)], and higher-level functional capacity [FFS (p for trend < 0.001); PFFS (p for trend < 0.001)]. After excluding PFFS data, the participants’ scores were associated with only higher-level functional capacity. Multi-regression analysis with higher-level functional capacity as the covariate showed that FFS and PFFS were significantly correlated with physical performance. Hence, improving food intake frequency, particularly that of high protein foods, and dietary variety may help maintain higher-level functional capacity and physical performance in community-dwelling older adults.
Nutrition, Physical Activity, and Dietary Supplementation to Prevent Bone Mineral Density Loss: A Food Pyramid
Authors: Rondanelli Mariangela , Faliva Milena Anna , Barrile Gaetan Claude , Cavioni Alessandro , Mansueto Francesca , Mazzola Giuseppe , Oberto Letizia , Patelli Zaira , Pirola Martina , Tartara Alice , Riva Antonella , Petrangolini Giovanna , Peroni Gabriella
Published: December 2021

Bone is a nutritionally modulated tissue. Given this background, aim of this review is to evaluate the latest data regarding ideal dietary approach in order to reduce bone mineral density loss and to construct a food pyramid that allows osteopenia/osteoporosis patients to easily figure out what to eat. The pyramid shows that carbohydrates should be consumed every day (3 portions of whole grains), together with fruits and vegetables (5 portions; orange-colored fruits and vegetables and green leafy vegetables are to be preferred), light yogurt (125 mL), skim milk (200 mL,) extra virgin olive oil (almost 20 mg/day), and calcium water (almost 1 l/day); weekly portions should include fish (4 portions), white meat (3 portions), legumes (2 portions), eggs (2 portions), cheeses (2 portions), and red or processed meats (once/week). At the top of the pyramid, there are two pennants: one green means that osteopenia/osteoporosis subjects need some personalized supplementation (if daily requirements cannot be satisfied through diet, calcium, vitamin D, boron, omega 3, and isoflavones supplementation could be an effective strategy with a great benefit/cost ratio), and one red means that there are some foods that are banned (salt, sugar, inorganic phosphate additives). Finally, three to four times per week of 30–40 min of aerobic and resistance exercises must be performed.
Lactiplantibacillus plantarum dfa1 Outperforms Enterococcus faecium dfa1 on Anti-Obesity in High Fat-Induced Obesity Mice Possibly through the Differences in Gut Dysbiosis Attenuation, despite the Similar Anti-Inflammatory Properties
Authors: Ondee Thunnicha , Pongpirul Krit , Janchot Kantima , Kanacharoen Suthicha , Lertmongkolaksorn Thanapat , Wongsaroj Lampet , Somboonna Naraporn , Ngamwongsatit Natharin , Leelahavanichkul Asada
Published: December 2021

Fat reduction and anti-inflammation are commonly claimed properties of probiotics. Lactiplantibacillus plantarum and Enterococcus faecium were tested in high fat-induced obesity mice and in vitro experiments. After 16 weeks of probiotics, L. plantarum dfa1 outperforms E. faecium dfa1 on the anti-obesity property as indicated by body weight, regional fat accumulation, serum cholesterol, inflammatory cytokines (in blood and colon tissue), and gut barrier defect (FITC-dextran assay). With fecal microbiome analysis, L. plantarum dfa1 but not E. faecium dfa1 reduced fecal abundance of pathogenic Proteobacteria without an alteration in total Gram-negative bacteria when compared with non-probiotics obese mice. With palmitic acid induction, the condition media from both probiotics similarly attenuated supernatant IL-8, improved enterocyte integrity and down-regulated cholesterol absorption-associated genes in Caco-2 cell (an enterocyte cell line) and reduced supernatant cytokines (TNF-α and IL-6) with normalization of cell energy status (extracellular flux analysis) in bone-marrow-derived macrophages. Due to the anti-inflammatory effect of the condition media of both probiotics on palmitic acid-activated enterocytes was neutralized by amylase, the active anti-inflammatory molecules might, partly, be exopolysaccharides. As L. plantarum dfa1 out-performed E. faecium dfa1 in anti-obesity property, possibly through the reduced fecal Proteobacteria, with a similar anti-inflammatory exopolysaccharide; L. plantarum is a potentially better option for anti-obesity than E. faecium.
Upregulation of Irisin and Vitamin D-Binding Protein Concentrations by Increasing Maternal 25-Hydrovitamin D Concentrations in Combination with Specific Genotypes of Vitamin D-Binding Protein Polymorphisms
Authors: Karras Spyridon N. , Dursun Erdinç , Alaylıoglu Merve , Gezen-Ak Duygu , Al Anouti Fatme , Pilz Stefan , Pludowski Pawel , Jude Edward , Kotsa Kalliopi
Published: December 2021

Dyshomeostasis of vitamin D-binding protein (VDBP) has been implicated in the pathogenesis of various pregnancy complications, including preeclampsia, preterm birth, gestational diabetes, and adverse metabolic profiles in the offspring. VDBP polymorphisms have been consistently reported to contribute to this intriguing interplay. Until recently, the effects of VDBP polymorphism heterogeneity on maternal and neonatal adipomyokine profiles have not been investigated, specifically after incorporating the different maternal and neonatal 25-hydroxyvitamin D concentration cut-offs at birth. We aimed to investigate the potential effects of maternal and neonatal VDBP polymorphisms on adiponectin, irisin, and VDBP concentrations at birth, according to different cut-offs of vitamin D status, in maternal–neonatal dyads recruited from the sunny region of Northern Greece. We obtained blood samples from 66 mother–child pairs at birth. Results indicated that (i) Neonatal serum biomarkers were not affected by any included neonatal VDBP polymorphism according to different cut-offs of neonatal vitamin D status at birth, (ii) neonatal VDBP concentration was elevated in neonates with maternal rs7041 GG genotype, (iii) maternal 25(OH)D at ≤75 nmol/L resulted in increased concentrations of maternal VBDP and irisin concentrations in women with CC genotype for rs2298850 and rs4588,whereas this effect was also evident for this cut-off for neonatal VDBP concentrations at birth for GC genotype for rs 7041, and (iv) no significant effect of neonatal VDBP polymorphisms was observed on neonatal VDBP, adiponectin, or irisin levels when stratified according to maternal 25(OH)D cut-offs. In conclusion, these findings confirm that among women with the combination of CC genotype for rs2298850 and rs4588, a specific high cut-off of maternal 25(OH)D results in increasing maternal VBDP concentrations, hence providing a mechanistic rationale for aiming for specific cut-offs of vitamin D after supplementation during pregnancy, in daily clinical practice.
Effect of Different Types of Intermittent Fasting on Biochemical and Anthropometric Parameters among Patients with Metabolic-Associated Fatty Liver Disease (MAFLD)—A Systematic Review
Authors: Różański Gracjan , Pheby Derek , Newton Julia L. , Murovska Modra , Zalewski Paweł , Słomko Joanna
Published: December 2021

Metabolic-associated fatty liver disease (MAFLD), previously called non-alcoholic fatty liver disease (NAFLD), is the most common chronic liver disease worldwide. It is characterised by excessive fat accumulation in hepatocytes. Currently, no pharmacological therapy is effective for this disease, so non-pharmacological alternatives such as diet, supplementation or physical activity are being sought. For this reason, we reviewed the available databases to analyse the studies conducted to date using different modifications of intermittent fasting among patients with MAFLD. Eight studies using this dietary strategy were included in this review. The results obtained in the different trials are varied and do not allow a clear determination of the effect of the different types of intermittent fasting on anthropometric and biochemical parameters among patients with MAFLD. However, this type of diet seems to show some therapeutic potential, but further studies are needed.
The Use of Bovine Colostrum in Medical Practice and Human Health: Current Evidence and Areas Requiring Further Examination
Authors: Playford Raymond John
Published: December 2021

Colostrum is produced by the mammary gland for the first few days following birth and is a rich natural source of macro- and micro-nutrients, immunoglobulins, and peptides with anti-microbial, immune modulatory and/or growth-factor activity [...]
Implication of Potential Differential Roles of the Two Phosphoglucomutase Isoforms in the Protozoan Parasite Cryptosporidium parvum
Authors: Nie Jiawen , Yin Jigang , Wang Dongqiang , Wang Chenchen , Zhu Guan
Published: December 2021

Phosphoglucomutase 1 (PGM1) catalyzes the conversion between glucose-1-phosphate and glucose-6-phosphate in the glycolysis/glucogenesis pathway. PGM1s are typically cytosolic enzymes in organisms lacking chloroplasts. However, the protozoan Cryptosporidium parasites possess two tandemly duplicated PGM1 genes evolved by a gene duplication after their split from other apicomplexans. Moreover, the downstream PGM1 isoform contains an N-terminal signal peptide, predicting a non-cytosolic location. Here we expressed recombinant proteins of the two PGM1 isoforms from the zoonotic Cryptosporidium parvum, namely CpPGM1A and CpPGM1B, and confirmed their enzyme activity. Both isoforms followed Michaelis–Menten kinetics towards glucose-1-phosphate (Km = 0.17 and 0.13 mM, Vmax = 7.30 and 2.76 μmol/min/mg, respectively). CpPGM1A and CpPGM1B genes were expressed in oocysts, sporozoites and intracellular parasites at a similar pattern of expression, however CpPGM1A was expressed at much higher levels than CpPGM1B. Immunofluorescence assay showed that CpPGM1A was present in the cytosol of sporozoites, however this was enriched towards the plasma membranes in the intracellular parasites; whereas CpPGM1B was mainly present under sporozoite pellicle, although relocated to the parasitophorous vacuole membrane in the intracellular development. These observations indicated that CpPGM1A played a house-keeping function, while CpPGM1B played a different biological role that remains to be defined by future investigations.