bluuebunny/crossref_metadata_2025 huggingface dataset:
Harnessing Metformin’s Immunomodulatory Effects on Immune Cells to Combat Breast Cancer
Authors: Petrovic Andjela , Jovanovic Ivan , Stojanovic Bojan , Dimitrijevic Stojanovic Milica , Stojanovic Bojana S. , Jurisevic Milena , Simovic Markovic Bojana , Jovanovic Marina , Jovanovic Milan , Jovanovic Mihailo , Gajovic Nevena
Published: May 2024

Metformin, a medication known for its anti-glycemic properties, also demonstrates potent immune system activation. In our study, using a 4T1 breast cancer model in BALB/C WT mice, we examined metformin’s impact on the functional phenotype of multiple immune cells, with a specific emphasis on natural killer T (NKT) cells due to their understudied role in this context. Metformin administration delayed the appearance and growth of carcinoma. Furthermore, metformin increased the percentage of IFN-γ+ NKT cells, and enhanced CD107a expression, as measured by MFI, while decreasing PD-1+, FoxP3+, and IL-10+ NKT cells in spleens of metformin-treated mice. In primary tumors, metformin increased the percentage of NKp46+ NKT cells and increased FasL expression, while lowering the percentages of FoxP3+, PD-1+, and IL-10-producing NKT cells and KLRG1 expression. Activation markers increased, and immunosuppressive markers declined in T cells from both the spleen and tumors. Furthermore, metformin decreased IL-10+ and FoxP3+ Tregs, along with Gr-1+ myeloid-derived suppressor cells (MDSCs) in spleens, and in tumor tissue, it decreased IL-10+ and FoxP3+ Tregs, Gr-1+, NF-κB+, and iNOS+ MDSCs, and iNOS+ dendritic cells (DCs), while increasing the DCs quantity. Additionally, increased expression levels of MIP1a, STAT4, and NFAT in splenocytes were found. These comprehensive findings illustrate metformin’s broad immunomodulatory impact across a variety of immune cells, including stimulating NKT cells and T cells, while inhibiting Tregs and MDSCs. This dynamic modulation may potentiate its use in cancer immunotherapy, highlighting its potential to modulate the tumor microenvironment across a spectrum of immune cell types.
ADAM12-Generated Basigin Ectodomain Binds β1 Integrin and Enhances the Expression of Cancer-Related Extracellular Matrix Proteins
Authors: Mygind Kasper J. , Nikodemus Denise , Gnosa Sebastian , Kweder Ramya , Albrechtsen Nicolai J. Wewer , Kveiborg Marie , Erler Janine T. , Albrechtsen Reidar
Published: May 2024

Desmoplasia is a common feature of aggressive cancers, driven by a complex interplay of protein production and degradation. Basigin is a type 1 integral membrane receptor secreted in exosomes or released by ectodomain shedding from the cell surface. Given that soluble basigin is increased in the circulation of patients with a poor cancer prognosis, we explored the putative role of the ADAM12-generated basigin ectodomain in cancer progression. We show that recombinant basigin ectodomain binds β1 integrin and stimulates gelatin degradation and the migration of cancer cells in a matrix metalloproteinase (MMP)- and β1-integrin-dependent manner. Subsequent in vitro and in vivo experiments demonstrated the altered expression of extracellular matrix proteins, including fibronectin and collagen type 5. Thus, we found increased deposits of collagen type 5 in the stroma of nude mice tumors of the human tumor cell line MCF7 expressing ADAM12—mimicking the desmoplastic response seen in human cancer. Our findings indicate a feedback loop between ADAM12 expression, basigin shedding, TGFβ signaling, and extracellular matrix (ECM) remodeling, which could be a mechanism by which ADAM12-generated basigin ectodomain contributes to the regulation of desmoplasia, a key feature in human cancer progression.
Bioinformatics Analysis and Validation of Potential Markers Associated with Prediction and Prognosis of Gastric Cancer
Authors: Matsuoka Tasuku , Yashiro Masakazu
Published: May 2024

Gastric cancer (GC) is one of the most common cancers worldwide. Most patients are diagnosed at the progressive stage of the disease, and current anticancer drug advancements are still lacking. Therefore, it is crucial to find relevant biomarkers with the accurate prediction of prognoses and good predictive accuracy to select appropriate patients with GC. Recent advances in molecular profiling technologies, including genomics, epigenomics, transcriptomics, proteomics, and metabolomics, have enabled the approach of GC biology at multiple levels of omics interaction networks. Systemic biological analyses, such as computational inference of “big data” and advanced bioinformatic approaches, are emerging to identify the key molecular biomarkers of GC, which would benefit targeted therapies. This review summarizes the current status of how bioinformatics analysis contributes to biomarker discovery for prognosis and prediction of therapeutic efficacy in GC based on a search of the medical literature. We highlight emerging individual multi-omics datasets, such as genomics, epigenomics, transcriptomics, proteomics, and metabolomics, for validating putative markers. Finally, we discuss the current challenges and future perspectives to integrate multi-omics analysis for improving biomarker implementation. The practical integration of bioinformatics analysis and multi-omics datasets under complementary computational analysis is having a great impact on the search for predictive and prognostic biomarkers and may lead to an important revolution in treatment.
Oral Exposure to Titanium Dioxide E171 and Zinc Oxide Nanoparticles Induces Multi-Organ Damage in Rats: Role of Ceramide
Authors: Bautista-Pérez Rocío , Cano-Martínez Agustina , Herrera-Rodríguez Manuel Alejandro , Ramos-Godinez María del Pilar , Pérez Reyes Olga Lidia , Chirino Yolanda Irasema , Rodríguez Serrano Zariá José , López-Marure Rebeca
Published: May 2024

Food-grade titanium dioxide (E171) and zinc oxide nanoparticles (ZnO NPs) are common food additives for human consumption. We examined multi-organ toxicity of both compounds on Wistar rats orally exposed for 90 days. Rats were divided into three groups: (1) control (saline solution), (2) E171-exposed, and (3) ZnO NPs-exposed. Histological examination was performed with hematoxylin–eosin (HE) staining and transmission electron microscopy (TEM). Ceramide (Cer), 3-nitrotyrosine (NT), and lysosome-associated membrane protein 2 (LAMP-2) were detected by immunofluorescence. Relevant histological changes were observed: disorganization, inflammatory cell infiltration, and mitochondrial damage. Increased levels of Cer, NT, and LAMP-2 were observed in the liver, kidney, and brain of E171- and ZnO NPs-exposed rats, and in rat hearts exposed to ZnO NPs. E171 up-regulated Cer and NT levels in the aorta and heart, while ZnO NPs up-regulated them in the aorta. Both NPs increased LAMP-2 expression in the intestine. In conclusion, chronic oral exposure to metallic NPs causes multi-organ injury, reflecting how these food additives pose a threat to human health. Our results suggest how complex interplay between ROS, Cer, LAMP-2, and NT may modulate organ function during NP damage.
A Comprehensive Investigation of Stimulatory Agents on MAIT and Vα7.2+/CD161− T Cell Response and Effects of Immunomodulatory Drugs
Authors: Singh Parvind , Száraz-Széles Marianna , Baráth Sándor , Hevessy Zsuzsanna
Published: May 2024

Mucosal-associated invariant T (MAIT) cells, a subset of Vα7.2+ T cells, are a crucial link between innate and adaptive immunity, responding to various stimuli through TCR-dependent and independent pathways. We investigated the responses of MAIT cells and Vα7.2+/CD161− T cells to different stimuli and evaluated the effects of Cyclosporin A (CsA) and Vitamin D3 (VitD). Peripheral blood mononuclear cells (PBMCs) from healthy donors were stimulated with various agents (PMA/Ionomycin, 5-OP-RU, 5-OP-RU/IL-12/IL-33) with or without CsA and VitD. Flow cytometric analysis assessed surface markers and intracellular cytokine production. Under steady-state conditions, MAIT cells displayed elevated expression of CCR6 and IL-13. They showed upregulated activation and exhaustion markers after activation, producing IFNγ, TNFα, and TNFα/GzB. CsA significantly inhibited MAIT cell activation and cytokine production. Conversely, Vα7.2+/CD161− T cells exhibited distinct responses, showing negligible responses to 5-OP-RU ligand but increased cytokine production upon PMA stimulation. Our study underscores the distinct nature of MAIT cells compared to Vα7.2+/CD161− T cells, which resemble conventional T cells. CsA emerges as a potent immunosuppressive agent, inhibiting proinflammatory cytokine production in MAIT cells. At the same time, VitD supports MAIT cell activation and IL-13 production, shedding light on potential therapeutic avenues for immune modulation.
Revealing Hidden Genes in Botrytis cinerea: New Insights into Genes Involved in the Biosynthesis of Secondary Metabolites
Authors: Suárez Ivonne , Collado Isidro G. , Garrido Carlos
Published: May 2024

Utilizing bioinformatics tools, this study expands our understanding of secondary metabolism in Botrytis cinerea, identifying novel genes within polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS), sesquiterpene cyclase (STC), diterpene cyclase (DTC), and dimethylallyltryptophan synthase (DMATS) families. These findings enrich the genetic framework associated with B. cinerea’s pathogenicity and ecological adaptation, offering insights into uncharted metabolic pathways. Significantly, the discovery of previously unannotated genes provides new molecular targets for developing targeted antifungal strategies, promising to enhance crop protection and advance our understanding of fungal biochemistry. This research not only broadens the scope of known secondary metabolites but also opens avenues for future exploration into B. cinerea’s biosynthetic capabilities, potentially leading to novel antifungal compounds. Our work underscores the importance of integrating bioinformatics and genomics for fungal research, paving the way for sustainable agricultural practices by pinpointing precise molecular interventions against B. cinerea. This study sets a foundation for further investigations into the fungus’s secondary metabolism, with implications for biotechnology and crop disease management.
Enhancing Arabic Dialect Detection on Social Media: A Hybrid Model with an Attention Mechanism
Authors: Yafooz Wael M. S.
Published: May 2024

Recently, the widespread use of social media and easy access to the Internet have brought about a significant transformation in the type of textual data available on the Web. This change is particularly evident in Arabic language usage, as the growing number of users from diverse domains has led to a considerable influx of Arabic text in various dialects, each characterized by differences in morphology, syntax, vocabulary, and pronunciation. Consequently, researchers in language recognition and natural language processing have become increasingly interested in identifying Arabic dialects. Numerous methods have been proposed to recognize this informal data, owing to its crucial implications for several applications, such as sentiment analysis, topic modeling, text summarization, and machine translation. However, Arabic dialect identification is a significant challenge due to the vast diversity of the Arabic language in its dialects. This study introduces a novel hybrid machine and deep learning model, incorporating an attention mechanism for detecting and classifying Arabic dialects. Several experiments were conducted using a novel dataset that collected information from user-generated comments from Twitter of Arabic dialects, namely, Egyptian, Gulf, Jordanian, and Yemeni, to evaluate the effectiveness of the proposed model. The dataset comprises 34,905 rows extracted from Twitter, representing an unbalanced data distribution. The data annotation was performed by native speakers proficient in each dialect. The results demonstrate that the proposed model outperforms the performance of long short-term memory, bidirectional long short-term memory, and logistic regression models in dialect classification using different word representations as follows: term frequency-inverse document frequency, Word2Vec, and global vector for word representation.
Cyanide Addition to Diiron and Diruthenium Bis-Cyclopentadienyl Complexes with Bridging Hydrocarbyl Ligands
Authors: Cinci Alessia , Ciancaleoni Gianluca , Zacchini Stefano , Marchetti Fabio
Published: May 2024

We conducted a joint synthetic, spectroscopic and computational study to explore the reactivity towards cyanide (from Bu4NCN) of a series of dinuclear complexes based on the M2Cp2(CO)3 scaffold (M = Fe, Ru; Cp = η5-C5H5), namely [M2Cp2(CO)2(µ-CO){µ,η1:η2-CH=C=CMe2}]BF4 (1Fe-Ru), [Ru2Cp2(CO)2(µ-CO){µ,η1:η2-C(Ph)=CHPh}]BF4 (2Ru) and [M2Cp2(CO)2(µ-CO){µ-CN(Me)(R)}]CF3SO3 (3Fe-Ru). While the reaction of 1Fe with Bu4NCN resulted in prevalent allenyl deprotonation, preliminary CO-NCMe substitution in 1Ru enabled cyanide addition to both the allenyl ligand (resulting in the formation of a h1:h2-allene derivative, 5A) and the two metal centers (affording 5B1 and 5B2). The mixture of 5B1-2 was rapidly converted into 5A in heptane solution at 100 °C, with 5A being isolated with a total yield of 60%. Following carbonyl-chloride substitution in 2Ru, CN− was incorporated as a terminal ligand upon Cl− displacement, to give the alkenyl complex 6 (84%). The reactivity of 3Fe and 3Ru is strongly influenced by both the metal element, M, and the aminocarbyne substituent, R. Thus, 7aRu was obtained with a 74% yield from cyanide attack on the carbyne in 3aRu (R = Cy, cyclohexyl), whereas the reaction involving the diiron counterpart 3aFe yielded an unclean mixture of the metastable 7aFe and the CO/CN− substitution product 8aFe. The cyano-alkylidene complexes 7aRu (R = Cy) and 7bFe (R = Me) underwent CO loss and carbene to carbyne conversion in isopropanol at 60–80 °C, giving 8aRu (48%) and 8bFe (71%), respectively. The novel compounds 5A, 5B1-2, 6 and 7aRu were characterized by IR and NMR spectroscopy, with the structure of 7aRu further elucidated by single crystal X-ray diffraction analysis. Additionally, the DFT-optimized structures of potential isomers of 5A, 5B1-2 and 6 were calculated.
Dengue Virus Serotype 1 Effects on Mosquito Survival Differ among Geographically Distinct Aedes aegypti Populations
Authors: Keirsebelik Milan S. G. , David Mariana R. , Pavan Márcio Galvão , Couto-Lima Dinair , Palomino Miriam , Rahman Rafi Ur , Hoffmann Ary A. , Bahia Ana C. , Caljon Guy , Maciel-de-Freitas Rafael
Published: May 2024

The mosquito Aedes aegypti is distributed worldwide and is recognized as the primary vector for dengue in numerous countries. To investigate whether the fitness cost of a single DENV-1 isolate varies among populations, we selected four Ae. aegypti populations from distinct localities: Australia (AUS), Brazil (BRA), Pakistan (PAK), and Peru (PER). Utilizing simple methodologies, we concurrently assessed survival rates and fecundity. Overall, DENV-1 infection led to a significant decrease in mosquito survival rates, with the exception of the PER population. Furthermore, infected Ae. aegypti from PAK, the population with the lowest infection rate among those tested, exhibited a noteworthy reduction in egg laying. These findings collectively suggest that local mosquito-virus adaptations may influence dengue transmission in endemic settings.
Meleis’s Transition Theory in Gerontogeriatric Nursing and the Future Need for Specialized Care
Authors: Coelho Adriana , Lobão Catarina , Parola Vítor , Almeida Maria de Lurdes , Queirós Paulo , Gonçalves Rui , Sousa Joana Pereira , Neves Hugo
Published: May 2024

With an ageing global population, healthcare systems confront challenges unique to the elderly. Current care models primarily manage health–illness shifts but frequently miss developmental transitions, particularly for older adults. Meleis’s transition theory offers an underutilized framework to rejuvenate our gerontogeriatric nursing approach. This article aims to articulate and advocate the value of Meleis’s transition theory as a framework for gerontogeriatric nursing, focusing on developmental transitions, thereby seeking a transformative change in the quality of elderly care. The article delves into Meleis’s transition theory’s vital components—transition types, conditions, and nursing therapeutics. It explores how these can be a multifaceted guide for gauging and overseeing ageing’s developmental shifts; adopting this theoretical perspective deepens our comprehension and bears tangible implications. Nurses versed in this theory could appreciate the benefits of a nuanced approach to the elderly, distinguishing it from the conventional biomedical stance. As the demands of an ageing populace grow, the integration of Meleis’s transition theory into nursing practices is not merely beneficial but imperative, setting a new standard for comprehensive and specialized elderly care.
Congenital Gerbode Defect: A Left Ventricular to Right Atrial Shunt—State-of-the-Art Review of Its General Data, Diagnostic Modalities, and Treatment Strategies
Authors: Winter Lone , Strizek Brigitte , Recker Florian
Published: May 2024

The congenital Gerbode defect is defined as an abnormal communication between the left ventricle and the right atrium. This review aimed to summarize existing evidence, shed light on the clinical implications, and identify knowledge gaps. The systematic literature search was conducted in the PubMed and Google Scholar medical databases using specifically selected keywords. The inclusion of each publication was assessed according to predefined eligibility criteria based on the PICOM (Population, Phenomenon of Interest, Context, Methodology) schema. Titles and abstracts were screened independently by two authors. Available full-text versions of included publications were reviewed and relevant information was extracted. A total of 78 reports were included. The compilation of all congenital Gerbode defect cases described in the literature revealed a variety of clinical presentations comprising dyspnea, palpitations, growth retardation, and asymptomatology. A suitable multimodal diagnostic approach for newborns consists of auscultation, TTE, and optionally TEE and MRI. Because of its rarity, diversity of findings, unknown pathophysiology, and similarity to more common cardiac diseases, the diagnostic challenge remains significant. To prevent untreated long-term sequelae, early individualized treatment is recommended. Surgical defect closure is preferred to device closure for evidence reasons, although major developments are currently taking place. In conclusion, the congenital Gerbode defect provides a diagnostic challenge for pediatricians to allow early diagnosis and intervention in order to improve patients’ quality of life.
Sleep Bruxism and Sleep Structure in Comorbid Insomnia and Obstructive Sleep Apnea (COMISA) Syndrome: A Polysomnographic Study
Authors: Blaszczyk Bartlomiej , Meira e Cruz Miguel , Waliszewska-Prosol Marta , Wieckiewicz Mieszko , Nowacki Dorian , Kanclerska Justyna , Lachowicz Gabriella , Wojakowska Anna , Michalek-Zrabkowska Monika , Przegralek Jakub , Smardz Joanna , Antosz Katarzyna , Mazur Grzegorz , Martynowicz Helena
Published: May 2024

Introduction: Comorbid insomnia and obstructive sleep apnea (COMISA) is not a well-identified sleep disorder, despite having a significant impact on health. This study investigates the relationship between sleep bruxism (SB) and sleep architecture in patients with COMISA, obstructive sleep apnea (OSA), and in those without any sleep disorders. Methods: 119 patients were included in the study and divided into three groups: OSA, COMISA, and a control group. Polysomnographic (PSG) examination provided parameters related to sleep architecture, OSA, and characteristics of SB. Results: The bruxism episode index (BEI) and other SB parameters were not found to be statistically different between the three groups (p > 0.05). There was no statistical difference in measured sleep architecture between the COMISA and OSA groups (p > 0.05). In comparison to the control group, participants in the COMISA group were found to have an increased apnea–hypopnea index (AHI), oxygen desaturation index (ODI), respiratory disturbance index (RDI), all arousals (AA), and respiratory arousals (RA) (p < 0.05). Among COMISA patients, AA and RA were shown to have a positive linear correlation with the number of bradycardia events per hour (r = 0.49, r = 0.48, p < 0.05). Conclusions: SB does not occur in patients with COMISA more frequently than in patients with OSA or those without any sleep disorders. PSG parameters are not specific for COMISA; therefore, in order to differentiate this disorder from OSA alone, a comprehensive patient assessment has to be performed.
Unsupervised Cluster Analysis in Patients with Cardiorenal Syndromes: Identifying Vascular Aspects
Authors: de Freminville Jean-Baptiste , Halimi Jean-Michel , Maisons Valentin , Goudot Guillaume , Bisson Arnaud , Angoulvant Denis , Fauchier Laurent
Published: May 2024

Background/Objectives: Cardiorenal syndrome (CRS) is a disorder of the heart and kidneys, with one type of organ dysfunction affecting the other. The pathophysiology is complex, and its actual description has been questioned. We used clustering analysis to identify clinically relevant phenogroups among patients with CRS. Methods: Data for patients admitted from 1 January 2012 to 31 December 2012 were collected from the French national medico-administrative database. Patients with a diagnosis of heart failure and chronic kidney disease and at least 5 years of follow-up were included. Results: In total, 13,665 patients were included and four clusters were identified. Cluster 1 could be described as the vascular–diabetes cluster. It comprised 1930 patients (14.1%), among which 60% had diabetes, 94% had coronary artery disease (CAD), and 80% had peripheral artery disease (PAD). Cluster 2 could be described as the vascular cluster. It comprised 2487 patients (18.2%), among which 33% had diabetes, 85% had CAD, and 78% had PAD. Cluster 3 could be described as the metabolic cluster. It comprised 2163 patients (15.8%), among which 87% had diabetes, 67% dyslipidemia, and 62% obesity. Cluster 4 comprised 7085 patients (51.8%) and could be described as the low-vascular cluster. The vascular cluster was the only one associated with a higher risk of cardiovascular death (HR: 1.48 [1.32–1.66]). The metabolic cluster was associated with a higher risk of kidney replacement therapy (HR: 1.33 [1.17–1.51]). Conclusions: Our study supports a new classification of CRS based on the vascular aspect of pathophysiology differentiating microvascular or macrovascular lesions. These results could have an impact on patients’ medical treatment.
Comparing Homogenized Instantaneous Meals and Traditional Pureed Foods in Patients Affected by Dysphagia: A Pilot Study
Authors: Sukkar Samir Giuseppe , Lorenzoni Giulia , Carraro Alice , Angioletti Francesca , Gregori Dario
Published: May 2024

Background: Institutionalized individuals with dysphagia are particularly at risk for malnutrition. This study investigated two texture-modified models for patients with dysphagia, as follows: (i) traditional homemade pureed food (PF) and (ii) homogenized meals obtained from dehydrated and rehydrated instantaneous preparations (IPs). Methods: A retrospective pilot study was performed. It included patients affected by medium-severity dysphagia admitted to the nursing home “Sacra Famiglia” Institute of Cocquio Trevisago, Varese. The patients were aged 41–81 years old and all had complex disabilities. They underwent anthropometric and biochemical parameter assessments at baseline, as well as at two months and four months follow-up. Results: The study involved 30 patients, 15 received the IP meal. The comparison between the baseline and the follow-up did not show significant anthropometric and biochemical parameter differences. Conversely, the IP group reported significantly higher levels of consumption and satisfaction, evaluated using a modified Chernoff scale based on three levels of smiles, than the PF group. Conclusions: The present findings provide promising indications to improve the diet of patients affected by dysphagia, since meal satisfaction is a relevant factor that has been shown to be associated with better patient mood, motivation to eat, and adherence to prescribed diet.
German Experience with a Novel Balloon-Expandable Heart Valve Prosthesis for Transcatheter Aortic Valve Implantation—Outcomes of the MYLAND (MYvaL germAN stuDy) Study
Authors: Ubben Timm , Tigges Eike , Kim Won-Keun , Holzamer Andreas , Breitenbach Ingo , Sodian Ralf , Rothe Jürgen , Hochholzer Willibald , Hakmi Samer , Neumann Franz-Josef
Published: May 2024

Background: The primary objective of this study was to evaluate the initial experience in Germany with the Meril Myval™ (MM) transcatheter heart valve (THV) system for the treatment of severe symptomatic aortic valve stenosis. The MM THV is a novel balloon-expandable valve with an expanded sizing matrix. Contemporary patients undergoing TAVI with the established Edwards Sapien™ (ES) THV served as the comparator group. Methods: Between 1st March and 31 August 2020 a total of 134 patients (33% female, 80.1 ± 6.7 years; EuroScore II 4.7 ± 4.8) underwent TAVI with an MM (95% transfemoral) for severe aortic stenosis at six German tertiary care centers. Results: Correct positioning of the THV was achieved in 98.5% (n = 132). Mean aortic gradients (MPG) were reduced from 42 ± 14 mmHg to 11 ± 5 mmHg. Mild postprocedural paravalvular leak (PVL) was observed in 62% (n = 82) patients, whereas only one patient had more than mild PVL. New permanent pacemaker implantation (PPI) was indicated in 15 patients (11%). Major vascular complications occurred in 6.7% (n = 9) patients. The in-hospital combined incidence of all-cause death and stroke was 4.5% (n = 6). In the comparator group that included 268 patients, the 30-day incidences of PPI, major vascular complications, and the composite of all-cause death and stroke were 16%, 1.9%, and 7.1%, respectively; MPGs were reduced from 44 ± 15 mmHg to 12.8 ± 4.6 mmHg and the more than mild PVL occurred in 0.7%. Conclusions: The MM is a promising novel THV system, with performance comparable to the established ES THVs. These findings await confirmation by ongoing randomized trials.
The German Multicenter Registry for ME/CFS (MECFS-R)
Authors: Hieber Hannah , Pricoco Rafael , Gerrer Katrin , Heindrich Cornelia , Wiehler Katharina , Mihatsch Lorenz L. , Haegele Matthias , Schindler Daniela , Donath Quirin , Christa Catharina , Grabbe Annika , Kircher Alissa , Leone Ariane , Mueller Yvonne , Zietemann Hannah , Freitag Helma , Sotzny Franziska , Warlitz Cordula , Stojanov Silvia , Hattesohl Daniel B. R. , Hausruckinger Anna , Mittelstrass Kirstin , Scheibenbogen Carmen , Behrends Uta
Published: May 2024

Background: Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a debilitating multisystemic disease characterized by a complex, incompletely understood etiology. Methods: To facilitate future clinical and translational research, a multicenter German ME/CFS registry (MECFS-R) was established to collect comprehensive, longitudinal, clinical, epidemiological, and laboratory data from adults, adolescents, and children in a web-based multilayer-secured database. Results: Here, we present the research protocol and first results of a pilot cohort of 174 ME/CFS patients diagnosed at two specialized tertiary fatigue centers, including 130 (74.7%) adults (mean age 38.4; SD 12.6) and 43 (25.3%) pediatric patients (mean age 15.5; SD 4.2). A viral trigger was identified in 160/174 (92.0%) cases, with SARS-CoV-2 in almost half of them. Patients exhibited severe functional and social impairment, as reflected by a median Bell Score of 30.0 (IQR 30.0 to 40.0) and a poor health-related quality of life assessed with the Short Form-36 health survey, resulting in a mean score of 40.4 (SD 20.6) for physical function and 59.1 (SD 18.8) for mental health. Conclusions: The MECFS-R provides important clinical information on ME/CFS to research and healthcare institutions. Paired with a multicenter biobank, it facilitates research on pathogenesis, diagnostic markers, and treatment options. Trial registration: ClinicalTrials.gov NCT05778006.
Uterus Transplantation as Infertility Treatment in Gynecological Cancer Survivors: A Systematic Review
Authors: Tsarna Ermioni , Eleftheriades Anna , Matsas Alkis , Triantafyllidou Olga , Christopoulos Panagiotis
Published: May 2024

Background: The aim of this systematic review is to summarize the evidence regarding the acceptance of uterine transplantation as infertility treatment among gynecological cancer survivors, surgical and pregnancy outcomes post-transplantation for gynecological cancer survivors, as well as relevant adverse events. Methods: PubMed and Embase were searched for records published since 2000, and extensive reference screening was performed. Results: Out of 1901 unique records identified, 7 are included in this review; 4 examined the proportion of gynecological cancer survivors among applicants for uterine transplantation, 2 examined rejection rates, pregnancy rates, and outcomes after uterine transplantation among gynecological cancer survivors, and 2 reported the frequency of relevant adverse events. Among the applicants, 60/701 (8.6%) were gynecological cancer survivors, only 1 transplanted patient was a cervical cancer survivor and achieved two live births after eight embryo transfers, and 2/27 (7.4%) of uterus transplantation recipients were diagnosed with CIN post-transplantation. Conclusions: Uterus transplantation can be regarded as an infertility treatment for absolute uterine factor infertility (AUFI), although only one gynecological cancer survivor has received a uterus transplantation. The efficacy, safety, and ethical considerations for gynecological cancer survivors need to be addressed for uterine transplantation to become an infertility treatment option for AUFI among gynecological cancer survivors.
A π-Theorem-Based Advanced Scaling Methodology for Similarity Assessment of Marine Shafting Systems
Authors: Rossopoulos Georgios N. , Papadopoulos Christos I.
Published: May 2024

This paper introduces a rigorous and comprehensive approach to the assessment of marine shafting systems through the utilization of an advanced π-Theorem-based scaling methodology. Integrating journal-bearing similarity assessment and shaft-line scaling methodology with advanced dimensional analysis, the study aims to provide a methodology foundation for systematic replication and analysis of marine shafting systems through scaled models. The proposed scaling methodology ensures geometric and mechanical similarity in terms of shaft-line deflection, considering key scaling parameters such as shaft length, diameter, weight, loads, rotational speed, material properties, bearing locations, and offsets. The advanced dimensional analysis computes specific non-dimensional ratios to guarantee a close resemblance between a real-size system and a scaled lab model. The methodology is analytically derived and validated with numerical simulations for a case study, conducting comparative analysis, evaluating discrepancies, and utilizing the integrated framework for experimentation.
New Adaptive Super-Twisting Extended-State Observer-Based Sliding Mode Scheme with Application to FOWT Pitch Control
Authors: Ma Ronglin , Siaw Fei Lu , Thio Tzer Hwai Gilbert , Yang Wenxiang
Published: May 2024

This paper details the transformation of the velocity or position-tracking problem of a class of uncertain systems using finite time stability control for first-order uncertain systems. A new composite extended-state observer sliding mode (ESOSM) scheme is proposed, which includes an adaptive super-twisting-like ESO and an adaptive super-twisting controller. The adaptive super-twisting controller is implemented through a barrier function-based second-order sliding mode algorithm. To further reduce control chattering and improve control performance, the adaptive super-twisting-like ESO, which employs high-order terms in the super-twisting algorithm to accelerate convergence, is designed to observe the lumped uncertainty in real time. The advantages of the proposed scheme are verified by a numerical example and application with regard to floating offshore wind turbine (FOWT) pitch control. Compared with proportional integral (PI) and adaptive super-twisting sliding mode (ASTSM) schemes, better results are obtained in velocity tracking and fatigue load suppression. For the FOWT pitch control application, the platform roll, pitch, and yaw are decreased by 3%, 2%, and 4%, respectively, compared to the PI scheme at an average turbulent wind speed of 17 m/s and turbulence intensity of 17.27%.
Deblurring Network for UV Images of Colorless Chemicals Floating on Sea Surface
Authors: Huang Hui , Zhang Yu , Lin Chuansong , Lin Fuliang , Fang Yue , Song Hong
Published: May 2024

Automatic imaging monitoring technology has been developed as a mature solution to address oil spill accidents. However, when airborne equipment is used to monitor the area of leakage, there is a chance of relative motion between the equipment and the target, leading to blurred images. This can hinder subsequent monitoring tasks, such as the identification and segmentation of hazardous chemical targets. Therefore, it is crucial to deblur these images, as sharper images can substantially improve the precision and accuracy of these tasks. In light of this, this paper presents a novel multi-scale recurrent deblurring network, leveraging the Convolutional Block Attention Module (CBAM) attention mechanism, to restore clear images in an end-to-end manner. The CBAM attention mechanism has been incorporated, which learns features in both the spatial and channel domains, thereby enhancing the deblurring ability of the network by combining the attention mechanisms of multiple domains. To evaluate the effectiveness of the proposed method, we applied the deblurring network to our self-built dataset of blurred ultraviolet (UV) images of colorless chemicals floating on water surfaces. Compared to the SRN-Deblur deblurring network, the proposed approach yields improved PSNR and SSIM results. Moreover, when compared with the state-of-the-art DeblurGANv2 method, it performs better in the SSIM. These results demonstrate that the proposed multi-scale recurrent deblurring network, based on the CBAM attention mechanism, exhibits a superior deblurring performance.
Determining the Climatic Drivers for Wine Production in the Côa Region (Portugal) Using a Machine Learning Approach
Authors: Fraga Helder , Freitas Teresa R. , Moriondo Marco , Molitor Daniel , Santos João A.
Published: May 2024

The Côa region in inner-northern Portugal heavily relies on viticulture, which is a cornerstone of its economy and cultural identity. Understanding the intricate relationship between climatic variables and wine production (WP) is crucial for adapting management practices to changing climatic conditions. This study employs machine learning (ML), specifically random forest (RF) regression, to predict grapevine yields in the Côa region using high-resolution climate data for 2004–2020. SHAP (SHapley Additive exPlanations) values are used to potentially explain the non-linear relationships between climatic factors and WP. The results reveal a complex interplay between predictors and WP, with precipitation emerging as a key determinant. Higher precipitation levels in April positively impact WP by replenishing soil moisture ahead of flowering, while elevated precipitation and humidity levels in August have a negative effect, possibly due to late-season heavy rainfall damaging grapes or creating more favorable conditions for fungal pathogens. Moreover, warmer temperatures during the growing season and adequate solar radiation in winter months favor higher WP. However, excessive radiation during advanced growth stages can lead to negative effects, such as sunburn. This study underscores the importance of tailoring viticultural strategies to local climatic conditions and employing advanced analytical techniques such as SHAP values to interpret ML model predictions effectively. Furthermore, the research highlights the potential of ML models in climate change risk reduction associated with viticulture, specifically WP. By leveraging insights from ML and interpretability techniques, policymakers and stakeholders can develop adaptive strategies to safeguard viticultural livelihoods and stable WP in a changing climate, particularly in regions with a rich agrarian heritage, such as the Côa region.
Impact of Subtalar Distraction Arthrodesis on Ankle Joint: Radiological Insights from Modified Grice–Green Procedure
Authors: Artioli Elena , Mazzotti Antonio , Cassanelli Edoardo , Langone Laura , Astolfi Michele , Abdi Pejman , Zielli Simone Ottavio , Arceri Alberto , Faldini Cesare
Published: May 2024

Subtalar distraction arthrodesis (SDA) is a surgical procedure designed to treat hindfoot deformities associated with isolated subtalar joint arthritis. In 1996, Fitzgibbons was the first to observe that, in some cases, hindfoot fusion appeared to be associated with the development of tibiotalar valgus tilt. Since then, few studies have addressed this issue. Given that hindfoot fusion can be performed using various techniques, this study investigated the potential tibiotalar joint frontal or sagittal modifications resulting from the modified Grice–Green technique. All the consecutive patients who underwent the modified Grice–Green procedure were included. The patient records were reviewed to extract demographic data. Weight-bearing foot and ankle radiographs were assessed to measure the talar tilt angle and the tibiotalar ratio on the same picture archiving and communication system by three independent observers. A total of 69 patients met the criteria for inclusion. The mean talar tilt showed no substantial changes, since the increase from 1.46 ± 1.62 preoperatively to 1.93 ± 2.19 at a minimum of 8 months postoperatively was not statistically significant (p = 0.47). The average preoperative tibiotalar ratio significantly increased from 33.4 ± 4.4% to 35 ± 4% postoperatively (p = 0.007), although remaining within the normal range, indicating a possible realignment of the posterior facet of the subtalar joint. In conclusion, this study highlights the effectiveness of the modified Grice–Green procedure in achieving a favorable realignment without impacting the ankle joint, particularly regarding tibiotalar valgus tilt.
Mechanisms of Male Reproductive Sterility Triggered by Dysbiosis of Intestinal Microorganisms
Authors: Wei Mingbang , Liu Huaizhi , Wang Yu , Sun Mingyang , Shang Peng
Published: May 2024

The intestinal microbiota, comprised of bacteria, archaea, and phages, inhabits the gastrointestinal tract of the organism. Male reproductive sterility is currently a prominent topic in medical research. Increasing research suggests that gut microbiota dysbiosis can result in various reproductive health problems. This article specifically investigates the impact of gut microbiota dysbiosis on male reproductive infertility development. Gut microbiota imbalances can disrupt the immune system and immune cell metabolism, affecting testicular growth and sperm production. This dysfunction can compromise the levels of hormones produced and secreted by the endocrine glands, affecting male reproductive health. Furthermore, imbalance of the gut microbiota can disrupt the gut–brain–reproductive axis, resulting in male reproductive infertility. This article explores how the imbalance of the gut microbiota impacts male reproductive infertility through immune regulation, endocrine regulation, and interactions of the gut–brain–reproductive axis, concluding with recommendations for prevention and treatment.
The Extent to Which the Available Water Resources in Upper Egypt Can Be Affected by Climate Change
Authors: Ashour Mohamed A. , El Degwee Yousra A. , Hashem Radwa H. , Abdou Abdallah A. , Abu-Zaid Tarek S.
Published: May 2024

Over the past two decades, rapid climate change has severely impacted people’s lives globally, affecting their safety and sustainability. Water, a vital human resource, has been severely affected, with drought and high temperatures leading to desertification, the drying up of rivers and lakes, spontaneous fires in forests, and massive floods and torrents due to melting ice and rising sea and ocean surface water levels. The expected impacts of climate change on the Nile, Egypt’s primary water source, are significant. These impacts can vary across regions, depending on factors like local climate, socio-economic dynamics, topography, and environmental nature. Upper Egypt, characterized by arid and semi-arid regions, faces water scarcity and socio-economic development challenges. Climate change exacerbates these issues, posing significant threats to the region’s ecological sustainability and socio-economic development. Therefore, it is crucial to address these impacts to ensure the Nile’s continued vitality and sustainability. The study aims to analyze the climate change data over the past few decades, analyze its characteristics, and model its effects on Upper Egypt’s water sources. The study expected a big decrease in the water resources of the Nile. While what is currently occurring in terms of fluctuating rainfall rates between scarcity and severity contradicts the results of those studies, that is the best evidence of the need for further research and studies to obtain more reliable and consistent results with the reality that it may help decision-makers to develop scenarios to manage climate change effectively, preventing or reducing negative effects, and finding suitable alternatives. Studies predict a 10% decrease in Nile revenue at Aswan High Dam Lake by 2095, with some predicting a 30% increase. This lack of credibility underscores the need for more comprehensive studies.
The Influence of nc-AlCrTiN/α-BN Coatings on Increasing the Durability of WC/Co Cutting Inserts in the Inconel Alloy Machining Process
Authors: Kacprzyńska-Gołacka Joanna , Sowa Sylwia , Brudnias Rafał , Paćko Daniel , Słomka Zbigniew , Piasek Artur , Wieciński Piotr , Habrat Witold , Garbacz Halina , Kopia Agnieszka , Smolik Jerzy
Published: May 2024

Anti-wear coatings obtained through PVD methods may significantly increase the durability of cutting tools by impacting their wear mechanisms. This study presents and discusses the results of studies on the impact of the thermal conductivity of PVD coatings on the intensity of the built-up edge (BUE) and built-up layer (BUL) formation in Inconel 600 alloy machining processes. The authors determine the microstructure, phase structure, mechanical properties (hardness, Young’s modulus, and adhesion), and thermal conductivity of different PVD coatings selected for the purpose of the study and varying in terms of conductivity—i.e., AlCrTiN and AlCrTiN/BN. Machining processes were carried out under controlled conditions using VBGT160404-M3 cutting inserts with AlCrTiN and AlCrTiN/BN coatings deposited on their surface. The authors prove that the adjustment of the thermal conductivity of PVD coatings to the thermal conductivity of the tool and machined materials can help change the direction of heat flow to cool the cutting zone more effectively. The study results presented in this article show that the deposition of the AlCrTiN/BN coating reduces the friction wear on the tool flank by over 70% and lowers the intensity of BUE and BUL formation processes on the face by 10%, compared to the AlCrTiN coating.
Experimental Simulation of Directional Crystallization of SiMo Cast Iron Alloyed with Al and Cr
Authors: Morgiel Krzysztof , Kopyciński Dariusz
Published: May 2024

SiMo ductile cast iron combines ease of part fabrication with good mechanical properties, including a usable plasticity range. Its poor corrosion resistance inherited from grey cast iron could be alleviated through alloying with Al or Cr additions capable of forming a dense oxide scale protecting the substrate. However, the presence of Al and Cr in cast iron tends to make the material brittle, and their optimum alloying additions need to be studied further. The present work was aimed at investigating the effect of crystallization rates on microstructure changes during directional crystallization of SiMo-type alloys with up to 3.5% Al and 2.4% Cr. The experiment was performed using the Bridgman–Stockbarger method. The tubular crucible was transferred from the hot section to cold section at rates ranging from 5 mm/h to 30 mm/h with a 4/5 crucible length and then quenched. The introduced Al promoted graphitization up to a point, wherein, at the highest applied addition, the graphite precipitation preceded crystallization of the rest of the melt. A rising level of Cr in these alloys from 1% to 2.4% resulted in the formation of low and high contents of pearlite, respectively. The higher crystallization rates proved effective in increasing the ferrite content at the expense of pearlite. In the investigated cast iron samples with smaller applied alloying additions, Widmanstätten ferrite or ausferrite, i.e., fine acircular phase, were often found. The switch from directional crystallization to quenching caused a transition from a liquid to solid state, which started with nucleation of islands of fine austenite dendrites with chunky graphite eutectic separating them. As these islands expanded, they pushed alloying additions to their sides, promoting carbide or pearlite formation in these places and forming a super-cell-like structure. The performed experiments helped gather information concerning the sensitivity of the microstructure of SiMo cast iron modified with Al and Cr to crystallization rates prevailing in heavy cast structures.
Active Vibration Avoidance Method for Variable Speed Welding in Robotic Friction Stir Welding Based on Constant Heat Input
Authors: Zong Guanchen , Kang Cunfeng , Chen Shujun
Published: May 2024

Robotic Friction Stir Welding (RFSW) technology integrates the advantages of friction stir welding and industrial robots, finding extensive applications and research in aerospace, shipbuilding, and new energy vehicles. However, the high-speed rotational process of friction stir welding combined with the low stiffness characteristics of serial industrial robots inevitably introduces vibrations during the welding process. This paper investigates the vibration patterns and impacts during the RFSW process and proposes an active vibration avoidance control method for variable speed welding based on constant heat input. This method utilizes a vibration feedback strategy that adjusts the spindle speed actively if the end-effector’s vibration exceeds a threshold, thereby avoiding the modal frequencies of the robot at its current pose. Concurrently, it calculates and adjusts the welding speed of the robot according to the thermal equilibrium equation to maintain constant heat input. A simplified dynamic model of the RFSW robot was established, and the feasibility of this method was validated through simulation experiments. This study fills the gap in vibration analysis of RFSW and provides new insights into control strategies and process optimization for robotic friction stir welding.