bluuebunny/crossref_metadata_2025 huggingface dataset:
Apoptosis antagonizing transcription factor expression and its validation as a potential diagnostic and prognostic biomarker in oral squamous cell carcinoma
Authors: Abudourousuli Ainiwaerjiang , Aierken Zumulaiti , Mamuti Hasiyati , Yimamu Tuxunayi , Da Chengli
Published: January 2025

Background

Oral squamous cell carcinoma (OSCC) is characterized by a high degree of malignancy and poor prognosis. This study aimed to investigate the expression of apoptosis antagonizing transcription factor (AATF) in OSCC, examine its correlation with clinicopathological features, assess its prognostic implications, and explore its potential role in OSCC progression.

Methods

Expression profiles and clinical data of OSCC patients were obtained from The Cancer Genome Atlas (TCGA). Immunohistochemical analysis on tissue microarrays was performed to assess AATF expression in OSCC. Functional enrichment analyses were conducted to identify potential signaling pathways and biological functions associated with AATF. Logistic regression analyses were employed to evaluate the relationship between AATF expression and clinicopathological features. Immune cell infiltration was assessed using single-sample gene set enrichment analysis (ssGSEA). The prognostic value of AATF was determined using Kaplan-Meier and Cox regression analyses. A nomogram was developed to predict overall survival (OS) rates at one, three-, and five years post-cancer diagnosis. Validation of AATF expression was performed using quantitative real-time PCR (qRT-PCR)

Results

AATF was significantly overexpressed in OSCC, and high AATF expression correlated with adverse clinicopathological features, including histologic grade and lymph node metastasis. Functional enrichment analysis revealed several enriched pathways, including epidermis development, immunoglobulin complex, antigen binding and IL-17 signaling pathway. Notably, AATF overexpression was negatively correlated with the infiltration levels of mast cells, interdigitating dendritic cells and Th 17 cells. High AATF expression significantly predicted poorer overall survival (OS) and disease-specific survival (DSS). Multivariate Cox analysis confirmed AATF as an independent negative prognostic marker of OS. Validation via qRT-PCR confirmed the overexpression of AATF in OSCC tissues.

Conclusion

Elevated expression of AATF in OSCC correlates with adverse clinicopathological features and negatively impacts immune cell infiltration. High AATF levels serve as an independent marker of poor OS and DSS. These findings support AATF as a valuable prognostic biomarker and a potential therapeutic target in OSCC, warranting further investigation.
Case Report: Button battery ingestion—an underestimated emergency in children
Authors: Konzett Karin , Gang Stefanie , Poyntner Lukas , Reithmeier Eberhard , Dertinger Susanne , Simma Burkhard
Published: January 2025

In general, the battery-related emergency department visit rate has continued to rise in the last decade. We present two cases of previously healthy toddlers (14 and 24 months old) with unwitnessed battery ingestion, who presented with massive, hematocrit-relevant hematemesis. Initially, both children showed stable vital signs. Following a symptom-free interval, both had a recurrence of massive hematemesis, which could not be controlled despite a multidisciplinary approach with pediatric, radiology, ENT specialists, endoscopy and anesthesia. Pathological workup showed necrosis with secondary aortoesophageal fistula due to battery-induced colliquation necrosis caused by caustic soda produced at the minus pole. We conclude, that preclinical risk scores, excellent clinical pathways (e.g., from Children's Hospital of Philadelphia) and detailed approaches from the National Capital Poison Center in the USA and also the European Society of Pediatric Gastroenterology Hepatology and Nutrition (ESPGHAN) offer clear and concise instructions for the management of button battery ingestion, but clinical awareness for vascular complications must be heightened. A multidisciplinary treatment algorithm for this fatal complication should be implemented and trained in pertinent hospitals. Moreover, it is of great importance to raise awareness for button battery ingestion in educational campaigns for parents and caregivers.
The role of health literacy in intervention studies targeting children living with overweight or obesity and their parents—a systematic mixed methods review
Authors: Holmen Heidi , Flølo Tone Nygaard , Tørris Christine , Torbjørnsen Astrid , Almendingen Kari , Riiser Kirsti
Published: January 2025

Background

Excess weight and obesity are increasing among children. Health literacy has been suggested as a feasible concept for enabling informed health choices in weight management interventions for children and their parents. Knowledge of the skills necessary for a child to maintain new health behaviors is limited and the role of health literacy remains unclear. Thus, there is a need to summarize the effects of and experiences with interventions that include health literacy components to guide the development of effective, future weight-related interventions.

Aim

This review aims to identify how health literacy is integrated into studies of interventions targeting children with excess weight or obesity and/or their parents and to appraise the identified literature.

Methods

We conducted a systematic mixed methods review, with searches in Medline, CINAHL, Cochrane, EMBASE, ERIC, PsycINFO, and Web of Science. We included studies of interventions published after 2013 that targeted children under 19 years with excess weight or obesity and/or their parents, where health literacy played a role. Results from the included studies were integrated using qualitative data transformation techniques, followed by a narrative summary.

Results

We identified 7,910 citations. Four reports met our inclusion criteria and were included for review. These reports included a total of 402 children. Health literacy was assessed at baseline in two studies and measured as an outcome over time in one study. Methodological quality varied among the retained reports, with differences observed in study design, risk of bias and data collection methods. The reports highlight the need to adapt weight management treatments to the individual level of health literacy in children and their families to first ensure active participation in their treatment and second ensure long-term compliance with necessary lifestyle-related changes.

Discussion

Surprisingly, little attention has been paid to the importance of health literacy in weight management programs targeting children and their families. Seemingly, treatments tailored to the individual level of health literacy have not been prioritized in research. Addressing health literacy in children's weight management continues to be a multifaceted and ambitious mission. Future research should focus on integrating health literacy into weight management interventions in a systematic and theory-driven manner, ensuring that these interventions are tailored to the specific needs of children and their families and can sustain behavior change over time.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=478957, identifier: CRD42023478957.
Hidden cause of paralysis: tight filum terminale in spinal cord injury without radiographic abnormality
Authors: Yuan Yi , An Zong Jian , Gao Fei , Li Zhi Hui , Xu Wei Li , Sun Yong
Published: January 2025

Objective

To explore the clinical characteristics and surgical treatment outcomes of spinal cord injury without radiographic abnormality (SCIWORA) in children.

Methods

A retrospective analysis was conducted on the clinical data of four children diagnosed with SCIWORA who were admitted to the Neurosurgery Department of Qingdao Women and Children's Hospital from November 2022 to June 2024. All four pediatric patients underwent laminectomy for spinal canal decompression along with resection of the filum terminale. Postoperatively, a regimen including corticosteroids and mannitol was administered. Following hospital discharge, each child was subjected to clinical follow-ups, and the neurological recovery from spinal cord injury was evaluated utilizing the American Spinal Injury Association (ASIA) impairment scale.

Results

Among the four patients, there was one male and three females, aged from 3 years and 2 months to 8 years. Two cases were due to low falls, one from a lower back injury, and one from a lumbar sprain. All patients had thoracolumbar injuries, with rapid progression of symptoms, including paralysis, sensory impairment, and urinary and fecal retention. Follow-up duration ranged from 3 to 24 months; three patients showed varying degrees of recovery in muscle strength and/or sensory function and bowel and bladder control, while one showed no improvement. One patient developed scoliosis and another presented with neurogenic bladder.

Conclusion

Tethered cord syndrome may be a potential underlying cause of SCIWORA. For children with SCIWORA accompanied by tethered cord syndrome, we recommend early surgical intervention to perform laminectomy and release the tethered cord, which may aid in the recovery of neurological function.
Effect of β-blocker on clinical outcomes in patients with traumatic brain injury: a retrospective propensity-matched study
Authors: Zhang Yaxin , Liu Tingting , Ji Wenwen , Wang Guangdong
Published: January 2025

Background

Traumatic brain injury (TBI) represents a significant public health challenge due to its complex management. β-blockers may offer neuroprotective benefits, but their impact on TBI outcomes remains unclear. This study aims to evaluate the effect of β-blocker use on clinical outcomes in TBI patients.

Methods

This retrospective cohort study included adult TBI patients, categorized into β-blocker and non-β-blocker groups. Propensity score matching (PSM) was utilized to balance baseline characteristics. Mortality was assessed through the application of multivariable Cox regression models and Kaplan–Meier survival curves. Subgroup analyses examined the consistency of the results.

Results

A total of 1,516 patients were included in the study, with 750 receiving β-blocker therapy and 766 not receiving it. After PSM, 473 pairs of patients were matched. The analysis indicated that β-blockers significantly reduce 28-day mortality (HR 0.43, 95% CI: 0.31–0.60, P < 0.001). However, patients receiving β-blocker had considerably longer hospital stays (7.89 days vs. 5.45 days, P < 0.001) and ICU stays (2.94 days vs. 2.33 days, P < 0.001).

Conclusion

β-blocker therapy is associated with improved short-term outcomes in patients with TBI, particularly in those with mild (GCS 13–15) and severe (GCS 3–8) TBI. However, no significant benefit was observed in patients with moderate TBI (GCS 9–12). This therapy may also prolong hospital and ICU stays.
Exploring the hemoglobin-to-red blood cell distribution width ratio (HRR) to peripheral arterial disease nexus: a comprehensive analysis of NHANES data from 1999 to 2004
Authors: Yu Zhihai , Lu Bin , Han Rui , Tu Can
Published: January 2025

Objective

This study aimed to investigate the correlation between the Hemoglobin-to-Red Blood Cell Distribution Width Ratio (HRR) and Peripheral Artery Disease (PAD) prevalence, utilizing data from the National Health and Nutrition Examination Survey (NHANES) between 1999 and 2004.

Methods

The study employed a cross-sectional design, analyzing data from 5,196 participants aged 40 and above. PAD was diagnosed using the Ankle-Brachial Index (ABI), with ABI less than 0.9 indicating PAD. HRR, calculated as the ratio of hemoglobin (HB) to red blood cell distribution width (RDW), was stratified into quartiles. Covariates included demographic and clinical variables such as BMI, lipid profiles, and diabetes status. Logistic regression analysis was conducted to assess the relationship between HRR and PAD, adjusting for potential confounders.

Results

The study found that higher HRR quartiles were associated with a decreased risk of PAD. After adjusting for confounders, the odds ratios for PAD in relation to the second, third, and fourth quartiles of HRR compared to the first quartile were 0.71, 0.62, and 0.44, respectively (P < 0.001). A one-unit increase in HRR corresponded to a 56% reduction in the probability of PAD. ROC analysis indicated HRR as a stronger protective factor for PAD compared to other variables. Stratified analyses revealed that younger age and lower BMI amplified the protective effect of HRR on PAD.

Conclusion

The study demonstrated a significant inverse relationship between HRR and PAD, suggesting that HRR may serve as a protective factor against PAD. This finding highlights the potential role of HRR in the pathogenesis of PAD and its clinical implications.
Activation of the periaqueductal gray controls respiratory output through a distributed brain network
Authors: Prostebby Mitchell , Saini Jashan , Biancardi Vivian , Dickson Clayton T. , Pagliardini Silvia
Published: January 2025

Introduction

The periaqueductal gray (PAG) has been previously established to play a key role in producing the vital changes in respiration occurring in response to threat. However, it is not fully understood how PAG activation alters the ongoing respiratory output, nor it is understood which pathways mediate these effects, as several regions have been previously identified to influence respiratory activity.

Methods

We used optogenetic tools in conjunction with EMG recordings of inspiratory and expiratory musculature to determine how PAG activation on short (250 ms) and longer (10–15 s) timescales alters respiratory muscle activity. Through cFOS mapping, we also identified key downstream brain regions which were likely modulated by PAG activation including the preBötzinger Complex (preBötC) and the lateral parafacial area (pFL). We then stimulated PAG terminals in those regions to determine whether their activity can account for the observed effects of PAG stimulation.

Results

Directly stimulating the PAG resulted in prominent changes to all recorded muscle activities and reset the breathing rhythm in either a phase-independent or phase-dependent manner. In contrast, stimulating PAG terminals in either preBötC or pFL with long or shorter timescale stimuli could not completely replicate the effects of direct PAG stimulation and also did not produce any respiratory reset.

Conclusions

Our results show that the effects of PAG activity on respiration are not mediated solely by PAG inputs to either the preBötC or pFL and more likely involve integration across a larger network of brainstem areas.
Construction and evaluation of Brassica rapa orphan genes overexpression library
Authors: Jiang Mingliang , Zhan Zongxiang , Li Xiaonan , Piao Zhongyun
Published: January 2025

Orphan genes (OGs) are crucial for species-specific characteristics and stress responses and are restricted to a specific taxon. However, their functions within particular species are poorly understood. Previous research identified OGs in Brassica rapa (BrOGs). In this study, the BrOGs overexpression (BrOGsOE) library in Arabidopsis thaliana was constructed. Approximately 128 unknown functional BrOGs were selected from Chinese cabbage and were overexpressed. The analysis focused on the phenotypes of leaf morphology and flowering time against phenotypic differences between Chinese cabbage and Arabidopsis. Interestingly, 72.66% of the transgenic lines showed distinctive phenotypic changes. Chinese cabbage-specific features, including curved, hairy, upward or downward-curving leaves, serrated margins, and multiple leaves, were observed in the BrOGsOE lines. The BrOGs overexpression library was associated with numerous variations in flowering time, particularly delayed flowering. This suggested that the delayed flowering time caused by BrOGs may be associated with resistance to bolting seem in Chinese cabbage. Furthermore, the results of stress treatment of 24 BrOGsOE lines with no apparent significant phenotypes suggested that a number of BrOGs have both general and specific functions against environmental and pathogenic stress. The findings of this study provide a comprehensive overview of the roles of BrOGs, emphasizing their significance as a resource for identifying positive genes associated with species-specific characteristics and stress responses and offering a solid foundation for the functional analysis of BrOGs.
From news images to action: the mobilizing effect of emotional protest images in news coverage
Authors: Geise Stephanie , Panke Diana , Heck Axel
Published: January 2025

The political power of images has probably never been stronger than in today's “information age” in which mobile devices allow instant access to news coverage of local, national, and global events, which are generally visualized in some way. Thus, this paper investigates whether political news images mobilize people to engage in political protest by appealing to their emotions. A pre-post-design integrating eye tracking with 143 participants examines how the observation of protest images in news coverage induces emotions and affects participatory intents. This reveals that a longer image observation activates both positive and negative discrete emotions (i.e., fascination, interest, sadness, anger, disgust, shame, guilt, and being touched) which increase the image recipients' willingness to participate politically. Additionally, for people with a high level of political interest, longer exposure to an emotion-inducing news image increases their willingness to participate in political activism, while a low level of political interest produces a negative effect.
Institutionalized violence in schools and language displacement: the voices of Mapuche speakers and elders
Authors: Sanhueza Susan , Maldonado Fabiola , Díaz Claudio , Friz Miguel , Toloza Carolina Aroca , Cuevas Hector Torres
Published: January 2025

This article addresses language displacement as a result of the institutionalized violence experienced by the Mapuche people. The objective is to explore, through the voices of Mapuche speakers and elders, the institutionalized violence encountered in schools and its impact on the displacement of the Mapuzungun language. Through discussion groups, we explored ancestral knowledge, language loss, its intergenerational transmission, and how the teaching of Mapuzungun should be approached for future generations. The results show that the elders possess an immeasurable source of knowledge, being the primary bearers of the culture, and propose an epistemology centered on the unity of language and culture.
COVID-19 vaccination attitudes and acceptance among people with serious mental illness
Authors: Small William , Silva Caroline , Johnson Rachel , Betti Vincent , Nguyen Antoinette , Todd Lauren , Jacobowitz David , Lamberti J. Steven
Published: January 2025

Objective

This study examines attitudes towards COVID-19 vaccination among a diverse cohort of adults with serious mental illness (SMI), participant characteristics that are associated with vaccine acceptance, and barriers to COVID-19 vaccination among this population.

Methods

A 28-item questionnaire was administered to 185 adults with SMI receiving care at a university-based outpatient psychiatric clinic. Variables included demographics, health behaviors, and vaccination status. Chi-square tests were used for categorical demographic comparisons on binary COVID-19 vaccine status.

Results

Female participants were more likely to have received COVID-19 vaccination (77.6%) than male (55.7%) participants. White (73.3%) and Hispanic/Latino (81.8%) participants were more likely to have received vaccination than Black/African American (54.9%) participants. Participants who reported having seen a primary care provider (PCP) within the past two years were more likely to be vaccinated (72.1%) than those who had not (41.7%). Participants who reported having received an influenza vaccine in the past two years were more likely to be vaccinated (80.2%) than those who had not (41.8%). Participants who had not been vaccinated were more likely to report greater concerns about all potential barriers to vaccination, including concerns about side effects, cost, health effects, and distrust of clinicians and governments.

Conclusions

The overall vaccination rate of study participants with SMI was similar to that of the general population. Efforts to enhance engagement in primary care may help improve preventative health behaviors in people with SMI.
Exploring smallholder farmers’ access and participation in the Home Grown School Feeding Programme in selected counties of Kenya
Authors: Kamau Joyce , Okoyo Collins , Kanyui Tabitha , Mwandawiro Charles , Singh Samrat , Drake Lesley
Published: January 2025

Introduction

Smallholder farmers (SHFs) produce 80% of the total agricultural output in Kenya. The Home Grown School Feeding Programme (HGSFP) was designed to address short-term hunger among primary school children from food-insecure households, enhancing access to primary education while providing a market to SHFs through local procurement of food for schools. This study investigated SHF access and participation in the HGSFP market and the uptake of a mobile phone platform (MPP) in HGSFP procurement in Tharaka Nithi, Kitui and Kilifi Counties of Kenya.

Methodology

Descriptive cross-sectional study design was utilized and data were collected from SHFs, school teachers and farmer-based organizations (FBOs) within the schools’ locality using semi-structured questionnaires. A total of 378 SHFs, 92 FBOs and 70 school teachers were interviewed for the study. Data were analyzed using Stata version 16.

Results

The study revealed that SHFs (22.8%) and FBOs (37.5%) gained access to HGSFP market and sold produce of maize (92.9%) and beans (91.4%). The main channel used by SHFs to sell produce to schools was through the FBOs (61.6%) amidst challenges of lack of surplus to sell (53.2%), low prices (50.9%) and poor transport infrastructure (23.6%). HGSFP schools purchased most of their food requirements from traders/brokers through manual tendering (65%). The uptake of the MPP for procurement of food by HGSFP schools and FBOs was embraced and promising and was rated as faster to use (76.8%) and more transparent in HGSFP procurement (44.6%).

Conclusion

The study concluded that local procurement opportunities through FBOs were underutilized. We recommend more capacity-building support for SHFs and FBOs to increase their production and give them better opportunities to be key participants in the HGSFP market and other structured markets. The MPP should be adopted for the procurement of food for school meals for transparency and accountability. To maximize its benefits, it should be inclusive of all market players, especially traders/brokers and sufficient training should be provided to all stakeholders to participate fully in the HGSFP market.
Multimodal approach to public health interventions using EGG and mobile health technologies
Authors: Zhang Xiao , Liu Han , Sun Mingyang , Feng Shuangyi
Published: January 2025

Introduction

Public health interventions increasingly integrate multimodal data sources, such as Electroencephalogram (EEG) data, to enhance monitoring and predictive capabilities for mental health conditions. However, traditional models often face challenges with the complexity and high dimensionality of EEG signals. While recent advancements like Contrastive Language-lmage Pre-training(CLIP) models excel in cross-modal understanding, their application to EEG-based tasks remains limited due to the unique characteristics of EEG data.

Methods

In response, we introduce PH-CLIP (Public Health Contrastive Language-lmage Pretraining), a novel framework that combines CLIP's representational power with a multi-scale fusion mechanism designed specifically for EEG data within mobile health technologies. PH-CLIP employs hierarchical feature extraction to capture the temporal dynamics of EEG signals, aligning them with contextually relevant textual descriptions for improved public health insights. Through a multi-scale fusion layer, PH-CLIP enhances interpretability and robustness in EEG embeddings, thereby supporting more accurate and scalable interventions across diverse public health applications.

Results and discussion

Experimental results indicate that PH-CLIP achieves significant improvements in EEG classification accuracy and mental health prediction efficiency compared to leading EEG analysis models. This framework positions PH-CLIP as a transformative tool in public health monitoring, with the potential to advance large-scale mental health interventions through integrative mobile health technologies.
Factors associated with having a steady homosexual partner among men who have sex with men, based on internet recruitment
Authors: Zhang Peng , Chen Weiyong , Li Jing , Fu Yun , Wang Fang , Wang Xing , Yang Zhongrong
Published: January 2025

Objective

This study analyzed the status and factors associated with having a steady homosexual partner (HP) among men who have sex with men (MSM) to provide a scientific basis for developing prevention and control strategies for acquired immunodeficiency syndrome in this population.

Methods

A questionnaire survey was conducted among study participants who were MSM recruited online by a non-governmental organization in May 2024. The participants were divided into two groups based on whether they had a steady HP. Univariate and multivariate logistic regression analyses were used to evaluate the factors influencing whether the participants had a steady HP.

Results

We surveyed 604 participants;211 (34.93%) had a current steady HP. Multivariate logistic regression analysis showed that older participants having a steady HP (adjusted odds ratio [aOR]: 1.04; 95% confidence interval [CI]: 1.01–1.07)was associated with an average monthly income >6,000 Chinese yuan (aOR: 1.47; 95% CI: 1.01–2.16), a belief that using enhancers such as Rush Popper increases the human immunodeficiency virus (HIV) infection risk (aOR: 1.56; 95% CI: 1.02–2.41), identification as purely homosexual (aOR: 2.02; 95% CI: 1.37–2.99), having engaged in anal sex with a HP in the previous 6 months (aOR: 4.13; 95% CI:2.51–6.90), and knowing the HIV status of their HP (aOR: 1.88; 95% CI: 1.17–3.06). Participants less likely to have a steady HP had used condoms consistently during anal sex with a HP in the prior 6 months (aOR: 0.56; 95% CI: 0.35–0.90) and engaged in one-night stands with HPs (aOR: 0.44; 95% CI: 0.29–0.65).

Conclusion

A low proportion of participants had steady HPs (34.93%). Therefore, public education for MSM should be promoted to encourage individuals to improve their awareness of HIV infection risks. Sexual responsibility, including correct use and risks of enhancers like Rush Popper, and avoiding high-risk behaviors, such as one-night stands, is crucial to reducing the risks of HIV and sexually transmitted infections.
Self-trainable and adaptive sensor intelligence for selective data generation
Authors: Rezvani Arghavan , Huang Wenjun , Chen Hanning , Ni Yang , Imani Mohsen
Published: January 2025

With the increasing integration of machine learning into IoT devices, managing energy consumption and data transmission has become a critical challenge. Many IoT applications depend on complex computations performed on server-side infrastructure, necessitating efficient methods to reduce unnecessary data transmission. One promising solution involves deploying compact machine learning models near sensors, enabling intelligent identification and transmission of only relevant data frames. However, existing near-sensor models lack adaptability, as they require extensive pre-training and are often rigidly configured prior to deployment. This paper proposes a novel framework that fuses online learning, active learning, and knowledge distillation to enable adaptive, resource-efficient near-sensor intelligence. Our approach allows near-sensor models to dynamically fine-tune their parameters post-deployment using online learning, eliminating the need for extensive pre-labeling and training. Through a sequential training and execution process, the framework achieves continuous adaptability without prior knowledge of the deployment environment. To enhance performance while preserving model efficiency, we integrate knowledge distillation, enabling the transfer of critical insights from a larger teacher model to a compact student model. Additionally, active learning reduces the required training data while maintaining competitive performance. We validated our framework on both benchmark data from the MS COCO dataset and in a simulated IoT environment. The results demonstrate significant improvements in energy efficiency and data transmission optimization, highlighting the practical applicability of our method in real-world IoT scenarios.
Protein-templated metal nanoclusters for chemical sensing
Authors: Chu Han-Wei , Demissie Girum Getachew , Huang Chih-Ching , Anand Anisha
Published: January 2025

Metal nanoclusters (MNCs) possess unique optical properties, discrete energy levels, biocompatibility and photostability, making them pivotal photoluminescent probes in chemical sensing. While substantial work has addressed the synthesis, theoretical studies and applications of gold-, copper-, and silver-based MNCs, this review introduces fresh perspectives on how the nature and concentration of templates—particularly protein molecules—affect the optical properties, stability and sensing capabilities of MNCs. We delve into the merits of using protein templates for creating highly stable MNCs with tunable photoluminescence (PL), providing a detailed comparison with non-protein based systems. This review also unveils recent advancements in the photophysical characteristics and chemical sensing applications of protein-templated MNCs, setting it apart from previous reviews by focusing on cutting-edge innovations in template influence. Challenges and future prospects for protein-templated MNCs in chemical sensing are highlighted, marking critical pathways for upcoming research. This work not only integrates current knowledge but also identifies gaps and opportunities not covered extensively in earlier reviews, such as the nuanced effects of template variation on MNCs’ functional properties.
The prognostic significance of tertiary lymphoid structures in oral squamous cell carcinomas: a systematic review
Authors: Ribeiro V. , Teillaud J-L. , Dieu-Nosjean M-C. , Lescaille G. , Rochefort J.
Published: January 2025

Introduction

Upper aerodigestive tract cancers are prevalent, with a global incidence surpassing 500,000 new cases in 2018. Among these, oral squamous cell carcinomas (OSCC) constitute the majority. OSCC has a low 5-year survival rate due to late-stage diagnosis. Risk factors include alcohol and tobacco use. However, non-smokers and non-drinkers are also affected, especially young patients with tongue cancer. The impact of tumor microenvironment (TME) and tumor-infiltrating lymphocytes (TILs) on OSCC prognosis remains debated. Remarkably, Tertiary Lymphoid Structures (TLS) identified in solid tumors have shown associations with favorable outcomes, yet their prognostic significance in OSCC remains understudied.

Objective

Thus, this systematic review aims to explore the value of TLS in OSCC reported in the literature.

Method

A scoping review was conducted and six retrospective cohort studies involving 1,203 patients met the inclusion criteria.

Results

Predominantly male patients, with an average age of 49.3 years were included. Immunohistochemistry was the primary method to identify TLS, present in 21% up to 100% of cases. TLS were predominantly located in the peri-tumoral area (75.4%–84.8%) compared to the intra-tumoral area (33.8%–33.9%). Our review shows that the presence of TLS is associated with improved survival in OSCC.

Discussion

However, variations in TLS detection and classification methods across studies introduce potential biases, hindering direct comparisons between findings. For instance, reports that are based solely on examining HES-stained slides for TLS identification may raise reliability concerns. Standardization of methodologies is imperative to ensure consistency in criteria utilization, thereby facilitating meaningful data comparisons.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023428010, PROSPERO (CRD42023428010).
Monitoring changes of forest height in California
Authors: Favrichon Samuel , Lee Jake , Yang Yan , Dalagnol Ricardo , Wagner Fabien , Sagang Le Bienfaiteur , Saatchi Sassan
Published: January 2025

Forests of California are undergoing large-scale disturbances from wildfire and tree mortality, caused by frequent droughts, insect infestations, and human activities. Mapping and monitoring the structure of these forests at high spatial resolution provides the necessary data to better manage forest health, mitigate wildfire risks, and improve carbon sequestration. Here, we use LiDAR measurements of top of canopy height metric (RH98) from NASA’s Global Ecosystem Dynamics Investigation (GEDI) mission to map vegetation height across the entire California for two different time periods (2019–2020 and 2021–2022) and explore the impact of disturbance. Exploring the reliability of machine learning methods for temporal monitoring of forest is still a developing field. We train a deep neural network to predict forest height metrics at 10-m resolution from radar and optical satellite imagery. Model validation against independent airborne LiDAR data showed a R2≥0.65 for the top of canopy height outperforming existing GEDI-based height maps and with improved sensitivity for mapping tall trees (RH98 ≥ 50 m) across California. Height showed distinct spatial variations across forest types offering quantitative and spatial information to evaluate forest conditions. The model, trained on data from 2019 to 2020, showed a similar accuracy when applied to satellite imagery acquired in 2021–2022 allowing a robust detection of changes caused by natural and man-made disturbances of forest. Changes of height captured impacts of tree mortality and fire intensity, pointing to the influence of wildfire across landscapes. Fires caused more than 60% of the large forest disturbances between the two time periods. This study demonstrates the benefits of using locally trained ML models to rapidly modernize forest management techniques in the age of increasing climate risks.
Patient satisfaction with a clinically integrated sleep apnea care model vs. the current sleep care paradigm
Authors: Salinas Gregory D. , Cerenzia Wendy , Coleman Brandon , Thorndike Frances , Edington Samantha , Riney Heidi
Published: January 2025

Introduction

Sleep apnea can have severe negative health effects, including cardiovascular diseases, metabolic disorders, and decreased quality of life. Continuous positive airway pressure (CPAP) therapy is highly effective and the gold standard treatment for sleep apnea; however, traditionally fragmented sleep healthcare has resulted in low levels of treatment adoption and adherence. A recent white paper analysis of traditional health plan claims found that a comprehensive model significantly outperformed traditional health plans with higher rates of adoption (80 vs. 49%), adherence (62 vs. 25%), and persistence (53 vs. 11%) to CPAP therapy, which resulted in lower total healthcare costs. To understand the patient experience in these models of care, this study compared patient satisfaction between the traditional sleep care approach and a clinically integrated, comprehensive sleep care program.

Methods

A survey was developed to understand differences in the patient experience with the two different care models with respect to: access to sleep care, including time from initial appointment to seeing a sleep specialist, referral and insurance process; ease of sleep testing process and receiving a diagnosis; adoption, quality of education, and training with CPAP; ongoing adherence support with CPAP, and quality of life. Data were compared using descriptive statistics and Chi-square analyses.

Results

A significantly higher proportion of patients in the comprehensive model were satisfied with all measured points in the patient's journey. Notably, twice as many patients in the comprehensive model were very satisfied with: ease of navigating the testing process, time between diagnosis and CPAP adoption, insurance navigation for CPAP approval, and availability and level of ongoing CPAP support. Comprehensive care patients experienced fewer work disruptions due to sleep apnea: only 7% missed work in the past 3 months, compared to 58% in the traditional model.

Discussion

Overall, the study highlights the benefits of a comprehensive care model in improving patient satisfaction with their sleep apnea journey and overall quality of life for individuals with sleep apnea. Pairing this positive patient experience data with prior data from the same treatment model shows that removing obstacles within a patient's journey positively impacts satisfaction while simultaneously improving adherence rates and reducing total healthcare costs.
Nitrification inhibitor promotes fertilizer N stabilization in soil as organic forms during a growing season of maize: a field 15N tracer study
Authors: Quan Zhi , Li Shanlong , Liu Dong , Liu Chang , Xun Zhifeng , Chen Xin , Fang Yunting
Published: January 2025

There is limited knowledge regarding the impact of nitrification inhibitors (NIs) and straw application on fertilizer N retention and in-season release. We conducted a trial to study the transformation of 15N-labeled urea in soils during the growing season of maize. To facilitate multiple destructive samplings throughout the season, we utilized a larger plot (25 m2) and a lower abundance 15N-fertilizer (1.193%) than usual. Soil extractable mineral N, mineral fixed ammonium, and organic N (ON) recovered 20 ± 21% (mean ± standard deviation), 6 ± 5%, and 25 ± 6% of the applied fertilizer N across three sampling stages of the growing season. On average, the bioavailability of fertilizer N in extractable mineral form was four times higher than that of mineral fixed ammonium. In contrast, fertilizer-derived ON represented a relatively stable N pool, maintaining high content throughout the growing period and becoming the major form (82%–93%) in the pool of total soil 15N at the physiological maturity stage of maize. Moreover, the co-application of nitrapyrin (a type of NI) significantly promoted fertilizer N storage in the ON form while the effect of straw was not significant. In conclusion, the NI-induced promotion of fertilizer-derived ON likely plays a critical role in storing fertilizer N for subsequent cultivations, rather than providing N nutrients for crop uptake during the current season.
The effects on milk marketing of cooperative partnership of dairy farmers: a case study from Türkiye
Authors: Can Bahar Aydin , Engindeniz Sait
Published: January 2025

In the agricultural sector, dairy cattle farming is a sector with a significant economic impact. One of Türkiye’s priority goals in agricultural development is to raise awareness of organization among farmers. Cooperatives play a crucial role in ensuring the sustainability of farmers in production. For dairy cattle farmers, one of the most effective means to market their milk is through cooperatives. The aim of this study is to reveal the socioeconomic characteristics, cooperative status and the effect of the cooperative on milk marketing of dairy cattle farmers who are members of the cooperative in Derince district of Kocaeli province. The main material of the study consisted of the data obtained by questionnaire method from 61 farmers who are members of Cavuslu Village Agricultural Development Cooperative in Derince district of Kocaeli province, Türkiye. The research includes data obtained during the 2020 production period. Dairy cattle farms were divided into 3 groups according to the presence of dairy cows and analyzed. Logistic regression analysis was used to determine the factors affecting the marketing of milk produced by the dairy cattle farms through cooperatives. According to the results of the research, 57.38% of the farmers market their milk through cooperatives. On average, there are 4.94 heads of domestic breed, 11.78 heads of culture breed and 9.20 heads of crossbred cows in the farms. The average milk selling price is 0.37 USD/kg. It was determined that the most effective factor for farmers to give milk to the cooperative was the selling price of milk. Dairy cattle farms with fewer milking cows and those without cooling tanks are more likely to sell their milk to the cooperative. In conclusion, for cooperatives to play a more effective role in dairy cattle farming, it is necessary to increase the competitiveness of cooperatives and ensure their effectiveness in the milk market through the establishment of processing and packaging facilities in the region.
Effects of butyric acid glycerol ester supplementation on intestinal nutrient transporter and immune-related genes in broiler chickens challenged with Eimeria maxima
Authors: Kpodo Kouassi R. , Miska Katarzyna B. , Schreier Lori L. , Milliken Daniel J. , Proszkowiec-Weglarz Monika
Published: January 2025

Introduction

Coccidiosis negatively affects intestinal health and digestive functions; however, whether butyric acid glycerol ester (BE) can mitigate these negative effects in broiler chickens is unknown. The study objective was to determine the effects of BE on nutrient transporter and intestinal immune genes in chickens infected with Eimeria maxima (EM).

Methods

Ross male 708 chicks were fed diets supplemented with 0 (control, C) or 0.25% of BE. On day 21, half the chickens from each feeding group were infected with 0 or 103 EM sporulated oocysts creating four treatment groups (C, +EM, +BE, and BE + EM; n = 6/treatment group). Jejunal and ileal tissues were collected at days 7 and 10 post-infection (PI).

Results

EM infection reduced (P ≤ 0.02) nutrient transporter genes EAAT3, PEPT2, B°AT, GLUT2, GLUT5, and SGLT1 at days 7 PI in the jejunum and ileum and EAAT3, PEPT1, PEPT2, and B°AT at day 10 PI in the jejunum. The supplementation of BE increased CAT1 in the jejunum and PEPT1, GLUT2, and GLUT5 (P ≤ 0.04) in the ileum at day 10 PI. A BE x EM interaction was observed (P ≤ 0.02) where GLUT1 and GLUT2 were increased in the jejunum of +BE compared to C chickens at day 10 PI. Among the immune-related genes, EM reduced (P ≤ 0.0001) IgA in the jejunum but increased (P = 0.004) TGF-β4 in the jejunum and ileum at day 7 PI. The expression of pIgR was reduced, while TLR2 and TLR4 were increased in +EM compared to C chickens at day 7 PI. In addition, IgA was increased (P = 0.01) in the ileum of +BE compared to C chickens at day 10 PI.

Conclusion

The results of the study confirmed that Eimeria maxima reduced nutrient transporters and immune-related genes in the jejunum and ileum of chickens. However, although BE increased the expression of some genes in non-challenged chickens, its supplementation did not prevent the reduction in the expression of selected genes caused by EM infection.
Ferula (Ferula elaeochytris) as a phytoestrogen: Use of Ferula in laying hens
Authors: Özkök Arda Onur , Kılınç Gözde
Published: January 2025

This study aims to examine the effects of Ferula root powder (FRP) on performance, egg quality, egg oxidant/antioxidant levels, some serum hormone/biochemical parameters, and physical properties of the oviduct in laying hens. A total of 72 (8 replicates, 3 hens/subgroup) laying hens (Nick Brown, 30 weeks) were divided into three groups (FRP-0, FRP-1, FRP-2). During the 9-week trial, FRP-0 (control) was fed with a basal diet (16.88% crude protein, 2,725 kcal/kg metabolizable energy). FRP-1 and FRP-2 groups, however, were fed a diet supplemented with 1 g/kg and 2 g/kg FRP, respectively. The results showed that laying performance, serum hormone (estradiol, progesterone) levels, and some internal organ weights were not affected by FRP supplementation. In comparison to the control group, higher yolk height and yolk index were found in the FRP-added groups. The albumen pH was found to have decreased in FRP-2 group. DPPH radical scavenging activity increased in egg yolk. TBARs value decreased in FRP-1 and FRP-2 groups. Serum triglyceride and cholesterol levels decreased in group FRP-2. Moreover, a higher uterus length was found in the FRP-supplemented group. Given the results achieved, it was determined that FRP does not have a significant estrogenic effect. However, FRP can be utilized to prevent lipid oxidation and for its hypocholesterolemic effect.
Which is more effective in thawing frozen rooster sperm: varying temperature or duration?
Authors: Nizam Mustafa Yiğit , Selçuk Murat , Esin Burcu
Published: January 2025

Introduction

Cryopreservation of poultry sperm is crucial for preserving genetic diversity and protecting endangered breeds. Rooster sperm is highly sensitive to cryopreservation due to its high polyunsaturated fatty acid content, making it prone to damage during freezing and thawing. This study evaluated the effects of thawing temperatures and storage conditions on sperm quality, including motility, morphology, and viability.

Methods

Frozen rooster semen samples were thawed at 37°C for 30 seconds, 60°C for 5 seconds, or 72°C for 5 seconds and stored at 4°C for up to 48 hours. Sperm quality parameters, including motility, kinematic characteristics, abnormal morphology, and viability, were assessed at 0, 3, 6, 9, 12, 24, and 48 hours using a Computer-Assisted Semen Analyzer (CASA).

Results

Post-thaw motility varied significantly between thawing temperatures at 24 and 48 hours (p < 0.05). Progressive and rapid progressive motility also differed significantly at 24 hours (p < 0.05). Sperm viability showed statistical differences across thawing groups at 24 and 48 hours (p < 0.05), while morphological abnormalities were significant at 12 and 48 hours (p < 0.05). Across all groups, sperm quality parameters varied significantly at each time point (p < 0.05).

Discussion

Thawing at 37°C and storing at 4°C for up to 24 hours optimizes sperm motility and viability, minimizing cryodamage and ensuring functional preservation. This approach is effective for short-term storage and crucial for sustaining genetic diversity and fertility in poultry breeding programs.
Laparoscopic Versus Robotic Ventral Hernia Repair With Intraperitoneal Mesh: A Systematic Review and Meta-Analysis Comparing the Perioperative Outcomes Randomised Controlled Trials
Authors: Singh Anurag , Toh Wei H. , Elzahed Nada , Khera Goldie , Baig Mirza K. , Mihailescu Andrei , Sajid Muhammad S.
Published: January 2025

Objective

The objective of this meta-analysis is to compare the perioperative surgical outcomes and cost-effectiveness of robotic ventral hernia repair (RVHR) versus laparoscopic ventral hernia repair (LVHR) with intraperitoneal mesh.

Methods

Randomised control trials (RCTs) reporting perioperative outcomes and costs in patients undergoing RVHR versus LVHR were selected from medical electronic databases and meta-analysis was conducted in accordance with the guidelines of the Cochrane Collaboration using statistical software RevMan version 5.

Results

Four RCTs on 337 patients reporting perioperative outcomes and cost comparison were included. In the random effect model analysis, the duration of operation was shorter, and cost was lower in the LVHR group but with significant statistical heterogeneity [standardized mean difference (SMD) −48.07, 95%, CI (−78.06, −18.07), Z = 3.14, P = 0.002], [SMD 0.82, 95%, CI (−1.48, −0.16), Z = 2.45, P = 0.01]. However, the variables of hernia recurrence and surgical site complications were statistically similar in both groups without any statistical heterogeneity among the included studies [Risk Ratio (RR) 1.05, 95%, CI (0.22, 4.99), Z = 0.06, P = 0.95], [RR 0.85, 95%, CI (0.48, 1.50), Z = 0.55, P = 0.58].

Conclusion

This systematic review demonstrates that RVHR does not offer any superiority among the compared perioperative variables (Duration of operation, hernia recurrence and surgical site complications) and it is not cost-effective when compared to LVHR. Due to the paucity of the RCTs and significant heterogeneity among the compared variables, a major multi-centre RCT is needed to validate these findings.