bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of unidirectional loading pusher head structure on the deformation law of bionic egg-shaped shell parts in internal high-pressure bulging
Authors: sun zhiying , Shen Mingting , Shi Zhengxian , Cheng Huiming
Published: October 2023

A solid particle medium flexible bulging technology is proposed to solve the problems of low molding precision, poor stability, and uneven deformation that may occur when processing bionic egg-shaped shells by the traditional bulging process. By optimizing the mold parameters, such as the angle and height of the pusher head, the flow characteristics of the granular material are improved, resulting in improved machining accuracy and surface quality when molding egg-shaped plastic shells. In this paper, we utilize a ø0.8-1 mm yttria-stabilized zirconia ceramic bead to serve as a pressure-transfer medium during the granular media forming process. Several different shapes of pusher heads with cone angles of 45°, 60°, 75°, and 90° and heights of 10mm, 25mm, and 40mm were designed and manufactured. The numerical model of simulation was verified by the analysis of strain inhomogeneity coefficient, and the maximum thinning rate at the apex of bulging of the egg-shaped test was measured and calculated. The results show that the maximum thinning rate at the apex of bulging is the largest for the pusher head with 45° cone angle and 25mm height, and the strain distribution is the most uniform and the expansion effect is the best. The study shows that the optimization of the process parameters of the pusher head shape can effectively improve the uniformity of the strain distribution and thickness distribution during the egg-shaped shell forming process, thus improving the machining accuracy of the plastic deformation of the bionic egg-shaped tube test piece.
Pregnancy outcomes of Jehovah’s Witnesses: case series over fifteen years in a tertiary teaching hospital
Authors: Ng Vivian Wai Yan , Seto Mimi Tin Yan , Cheung Ka Wang
Published: October 2023

Background
More than 5000 Jehovah’s Witnesses resided in Hong Kong. Higher morbidity and mortality rates in pregnancy were associated with this specific group of women than the general population. We aim to assess the obstetric and neonatal outcomes of Jehovah’s Witnesses (JW) mothers in a teaching hospital and to evaluate the acceptance rate of blood fractions and blood transfusion alternatives.
Methods
This is a retrospective case-control study. Women who delivered from 1st January 2005 to 31st December 2020 were analysed from the electronic Clinical Data Analysis and Reporting System (CDARS). Patients who identified themselves as JW were reviewed. The immediate next age-matched non-JW patient in the antenatal care booking register was recruited as control.
Results
There were 83 JW women identified within the study period. 35 delivered overseas or in the private sector, leaving 48 women for analysis. 8 (16.7%) accepted blood transfusion; 11 (22.9%) accepted some minor fractions, 29 (60.4 %) refused all blood components. 42 (87.5%) had consultant review and anaesthesiologist consultation before delivery with confirmation of their advanced directives. JW mothers had similar haemoglobin levels to controls at different time points – at booking, third trimester, delivery and the postnatal period (p=0.53-0.857). Pregnant JW were more likely to receive iron supplements in the antenatal period (p=0.006) compared with age-matched control. In terms of obstetrics outcome, there was no difference in mode of delivery compared with control. 30 (62.5%) had normal vaginal deliveries, 4 (8.3 %) had instrumental births and 14 (29.1%) had Caesarean births in JW arm. There was no increase in blood loss at delivery for JW mothers (p=0.19). Neonatal outcome in terms of mean birthweight (3266 grams vs 3041 grams, p=0.188) and 5-min Apgar score (9.8 vs 9.9, p=0.448) were comparable between JW and non-JW group. There were no maternal deaths, no hysterectomy and no admissions to the adult intensive care unit in either arms.
Conclusion
Pregnant JW who received optimised obstetric care had similar outcomes to controls who were non-JW. The risk of morbidity could be mitigated with precautions and JW mothers should not be discriminated on the choice of blood product refusal.
Postponing Spinal Surgery in Patients Recovered from COVID-19 based on Coagulation Test Results, Effective or Ineffective Strategy?
Authors: Mohsenzadeh Mahdi , Zamani Majid , Mokhtari AliMohammad , Talaie Alireza , Sajjadi SeyedHadi , Pouladkhay Fatemeh
Published: October 2023

Background
The COVID-19 pandemic has posed numerous challenges across medical disciplines, including laboratory testing. Alterations in blood parameters caused by the virus have raised concerns regarding the reliability of certain laboratory tests. This study aimed to investigate whether relying on hematology test results to postpone surgeries remains a dependable practice, particularly in the context of spinal interventions.
Methods
A retrospective analysis was conducted on the medical records of 220 patients who had undergone spinal surgery between October 2021 and June 2023 and whose COVID-19 status was documented. Inclusion criteria encompassed individuals without a history of blood disorders, pregnancy, drug or smoking addiction, or recent surgical procedures. Data were extracted using a checklist and analyzed utilizing SPSS software. Descriptive methods and statistical tests, such as independent samples t-test and chi-square test, were employed, with statistical significance set at p < 0.05.
Results
Among the analyzed patients, 56.4% were male and 43.6% were female. Fusion surgery was performed on 82.7% of individuals, while laminectomy surgery was performed on 17.3%. Approximately 65.9% had no underlying conditions, and 67.3% had a history of prior COVID-19 infection. Postoperative thrombotic complications were observed in 2.2%. Comparisons between patients with and without coagulation complications revealed no significant differences in demographic information, history of COVID-19 infection, or coagulation test results. Furthermore, a comparison of D-dimer test outcomes between individuals with normal D-dimer levels (54 people) and those with levels exceeding 500 (55 people) demonstrated no significant distinction (P > 0.05).
Conclusion
Based on the findings, this study concludes that for major and extensive spinal surgeries in patients with a history of COVID-19, evaluating D-dimer test results does not offer significant assistance in decision-making.
The Relationship Between Alarm Fatigue with Clinical Competence in Neonatal Intensive Care Nurses in Kermanshah-Iran, 2022.
Authors: Begjani Jamaloddin , Moheb Nasim Bagheri , Haghani Shima , Takhtgahi Homa Babaei
Published: October 2023

Background: Inadequate qualifications of nurses are one of the predisposing factors for the occurrence of clinical errors in care units, which can lead to doubling the amount of care. On the other hand, false alarms reduce the response time and nurses' trust in alarms, which can cause them to suffer from alarm fatigue in the long run. This study was conducted with the aim of determining the relationship between alarm fatigue andclinical competence in neonatal intensive care nurses in Iran, Kermanshah, 2022.
Methods: In this cross-sectional study, 140 neonatal intensive care nurses were selected by the census method. The data collection tools included a demographic questionnaire, the “Nurse Competence Scale” and an “Alarm fatigue questionnaire”. Data were analysed using descriptive and analytical statistics.
Results: The average alarm fatigue score was 21.61 ± 7.45 and above average. Most of the researched nurses, that means 75% of them, had very good clinical qualifications. The average score of clinical competence in nurses was 174.76± 26.74 and was evaluated at a very good level. The clinical competence of nurses in the subscale of managing situations, with an average of 83.72 ±14.53, is the highest, and in the subscale of ensuring quality, with an average of 75.31± 18.26, the lowest average score has been obtained among other subscales of clinical competence. This study shows the relationship between alarm fatigue and clinical competence in the teaching–coaching subscale among nurses in the neonatal intensive care unit of Kermanshah city, so that with the increase in alarm fatigue in nurses, the clinical competence in the teaching–coaching subscale decreases (p= 0/019).
Conclusion: Clinicalcompetence was scored at a very good level, and alarm fatigue was above average. This study shows the relationship between alarm fatigue and clinical competence in the teaching–coaching subscale among NICU nurses. The findings showed that the clinical competence of nurses obtained the highest average score in the managing situations subscale and the lowest average score in the ensuring quality subscale. It is appropriate to take measures to strengthen the aspect of ensuring quality, which is mostly evidence-based nursing. Additionally, according to the needs of nurses in hospitals, more planning should be done to improve education. It is necessary to adopt solutions according to standard guidelines to reduce alarms and to monitor the physiological monitoring systems in hospitals because not paying attention to these issues leads to the fatigue of the alarms of nurses, which itself has many consequences for nurses and patients.
Effectiveness of a Social Justice Curriculum Intervention on Cultural Competency among Internal Medicine Residents
Authors: Trivedi Bhavi , Mares Adriana , Mukherjee Debabrata , Harindranathan Priya , Hechanova Aimee , Edwards Brian , Dwivedi Alok , Deoker Abhizith
Published: October 2023

Introduction:
There is a nationwide need to include social justice topics in medical education to reduce health disparities of culturally diverse patients. Cultural competence acts as a link between cultural diversity and social justice to foster health equity among patients. We implemented a Social Justice in Medicine curriculum in the Internal Medicine residency program to prepare cultural competencies for social justice among residents. We sought to investigate the impact of curriculum implementation on residents' cultural competency.
Methods
The social justice in medicine included a variety of educational strategies including online curriculum modules, immersive clinical experiences in continuity clinics, community engagement, a lecture series (Social Determinants of Health, social injustices, resident activism etc.), and the use of reflective practices (bias assessments, group discussions). We conducted a pre-post quasi-experimental study to evaluate the effect of implementation of an educational intervention on competency as measured by the Inventory for Assessing the Process of Cultural Competence Among Healthcare Professionals - Revised, a 25-item 4-point Likert scale. This instrument assesses cultural awareness, knowledge, skill, encounters, and desire. The effect size was summarized with mean difference (MD) and 95% confidence interval (CI).
Results
A total of 40 residents participated in the study. Overall 11.7% relative improvement in cultural competency was observed following the intervention (MD = 7.7; 95%CI: 4.72, 10.68, p < 0.001). The intervention improved all the domains of competency including awareness (MD = 0.93; 95%CI: -0.02, 1.87), p = 0.05), knowledge (MD = 2.20; 95%CI: 1.33, 3.07, p < 0.001), skill (MD = 1.90; 95%CI: 1.12, 2.68, p < 0.001), encounters (MD = 1.10; 95%CI: 0.23, 1.97, p = 0.014), and desire (MD = 1.58; 95%CI: 0.80, 2.35, p < 0.001).
Conclusions
The implementation of social justice in medicine curriculum improved the cultural competencies among internal medicine residents in our study. Future longitudinal studies are required to investigate challenges, barriers, and modes of implementation strategies in a larger setting.
Up-to-dateness of patient versions of clinical practice guidelines and potential influencing factors: a mixed-methods analysis
Authors: Hellbrecht Irma , Könsgen Nadja , Pieper Dawid , Prediger Barbara , Breuing Jessica
Published: October 2023

Background:
Clinical practice guidelines (CPGs) provide recommendations and are a fundamental part of clinical practice. Following and based on CPGs, many guideline organisations also produce patient versions of CPGs (PVGs). The main objective of this study was to perform a mixed-methods analysis on the up-to-dateness of German PVGs and potential methodological influence factors.
Methods:
A mixed-methods analysis was conducted. First, a literature search to identify PVGs was performed between October 2022 and January 2023. PVGs fulfilling the following criteria were included: published in German, free accessible, explicit link to corresponding CPG, final version, date of literature search of CPG and publication date of PVG available (no publication date restriction). Second, interviews with experts in the PVG development were conducted and analysed using qualitative content analysis (following Mayring) with MAXQDA software.
Results:
49 PVGs were included and analysed. A median of 36 months elapsed between the literature search of CPGs and the publication of PVGs. Six interviews were conducted and interviewees mostly perceived PVGs as up-to-date. However, they identified exceptions in the up-to-dateness depending on the topic or thematic chapters of PVGs. Interviewees mentioned different influencing factors such as the scientific progress and the editorial process of PVGs.
Conclusion:
Our findings shed light on potential issues with regard to the up-to-dateness of PVGs. In the context of a fast-moving evidence basis, it seems doubtful whether PVGs actually reflect the current state of knowledge, especially in fields with high research activity. However, some factors may not be modifiable because they essentially contribute to the quality assurance of PVGs. Further research is desirable to investigate which measures may be feasible to improve the up-to-dateness of PVGs.
Beta Diversity Assessment of Odonata Communities in Amazonian Streams with Altered Environments
Authors: Silva Samantha Ribeiro , de Lima Edgar Luiz , Gonçalves Myckey Kenzy e Silva , Oliveira-Junior José Max Barbosa , Dias-Silva Karina , Juen Leandro , Brasil Leandro Schlemmer
Published: October 2023

Beta diversity has been widely used in studies to understand the spatial distribution patterns of species and to assess the effects of anthropic impacts on aquatic environments. Dissimilarity indices, such as Sorensen and Jaccard, are widely used to calculate beta diversity, and show differences in their way of evaluating and valuing biodiversity components, which result in different patterns. Despite this variation, the efficiency and performance of beta diversity to measure environmental impacts in communities remains unexplored. In this study, we In this study, we evaluate changes in the composition of Odonata species adult in Amazonian streams using different beta diversity indices. The Odonata were sampling in 98 small streams in two regions of the eastern Amazon. We compared the beta diversity of preserved and altered streams in each region using the Sorensen and Jaccard indices. Our results show that all methods used are effective in detecting changes in Odonata species composition when comparing preserved and altered stream communities. However, the Sorensen index was more able to reflect these changes, possibly due to its lower sensitivity to rare species compared to the Jaccard index. Even with a subtle difference in detection power, we should consider it, especially when environmental changes are less pronounced, using methods with greater accuracy increases the probability of detecting the effects of human actions on natural communities.
Bioinformatic Analysis of Acute Respiratory Distress Syndrome and Sepsis Reveals Hypoxia Related Genes
Authors: Zhang Rui
Published: October 2023

Backgroud: Use bioinformatic analysis to identify hypoxia related genes in acute respiratory distress syndrome (ARDS) and sepsis.
Methods: Bioinformatic analysis was performed in GSE28750 of sepsis patients and GSE76293 of acute respiratory distress syndrome (ARDS) patients. Then intersected the hypoxia related genes and DEGs to obtain 11 hypoxia-related differentially expressed genes (HRDEGs). GO analysis and GSEA was used to find functional enrichment in diseases. Regulatory network and immunocyte infiltration analysis were performed to evaluate the mechanism in ARDS and sepsis.
Results：A total of 11 genes (CAPG, CKS2, CLU, FLVCR1, FUNDC1, HPSE, LCN2, MAPK14, PFKFB3, PLAC8 and TDRD9) were identified as hypoxia-related differentially expressed genes (HRDEGs). GO analysis and GSEA was used to find functional enrichment among HRDEGs. Regulatory network discovered several mechanisms in ARDS and sepsis, and provide correlation between HRDEGs. CIBERSORT found several HRDGEs were correlation with the abundances of immunocytes.
Conclusion: Several genes related to hypoxia in ARDS and sepsis were found, associated with pathological progress in disease.
Guggulsterone selectively modulates STAT-3, mTOR, and PPAR-gamma signalling in a methylmercury-exposed experimental neurotoxicity: Evidence from CSF, blood plasma and brain samples
Authors: Kumar Sumit , Mehan Sidharth , Khan Zuber , Gupta Ghanshyam Das
Published: October 2023

Amyotrophic lateral sclerosis (ALS) is a paralytic disease that damages the brain and spinal cord motor neurons. Several clinical and preclinical studies have found that methylmercury (MeHg+) causes ALS. In ALS, (MeHg+-induced neurotoxicity manifests as oligodendrocyte destruction; myelin basic protein (MBP) deficiency leads to axonal death. ALS development has been connected to an increase in signal transducer and activator of transcription-3 (STAT-3), a mammalian target of rapamycin (mTOR), and a decrease in peroxisome proliferator-activated receptor (PPAR)-gamma. Guggulsterone (GST), a plant-derived chemical produced from Commiphorawhighitii resin, has been found to protect against ALS by modulating these signalling pathways. Vitamin D3 (VitD3) deficiency has been related to oligodendrocyte precursor cells (OPC) damage, demyelination, and white matter deterioration, which results in motor neuron death. As a result, the primary goal of this work was to investigate the therapeutic potential of GST by altering STAT-3, mTOR, and PPAR-gamma levels in a MeHg+-exposed experimental model of ALS in adult rats. The GST30 and 60 mg/kg oral treatments significantly improved the behavioral, motor, and cognitive dysfunctions and increased remyelination, as proven by the Luxol Fast Blue stain (LFB), and reduced neuroinflammation as measured by histological examinations. Furthermore, the co-administration of VitD3 exhibits moderate efficacy when administered in combination with GST60. Our results show that GST protects neurons by decreasing STAT-3 and mTOR levels while increasing PPAR-gamma protein levels in ALS rats.
Enhancing ECG Signal Data through Denoising Features with Transformer Generative Adversarial Networks for Model Classification 1D-CNN
Authors: Yehezky Hendrico , Bustamam Alhadi , Hermawan Hermawan
Published: October 2023

An important component of telemedicine's remote cardiac health monitoring of patients is the use of artificial intelligence (AI) technology to detect electrocardiograph (ECG) signals. Failure to properly diagnose and treat abnormal ECG patterns caused by arrhythmia symptoms can result in a fatal outcome. Given that arrhythmia symptoms contribute significantly to noncommunicable cardiovascular disease (CVD), which is responsible for approximately 32% of global mortality, this concern becomes even more significant. The high sensitivity of ECG signals to both external and internal electrical disturbances makes accurate interpretation of these signals for arrhythmia detection challenging. An effective denoising technique is presented in this method as a substitute approach to reduce noise disturbances in ECG signal data and enhance the quality of the training data for AI detection models. This pre-processing technique combines a synthesis approach with Gaussian filtering, an auto-encoder-decoder (transformer), and generative adversarial networks (GANs). The MIT-BIH dataset is the subject of research for this study, which has been categorized into Normal, Atrial Premature, Premature Ventricular Contraction, Fusion of Ventricular and Normal, and Fusion of Paced and Normal. The research findings show that the quality of the synthesized data is almost identical to that of the original data. It is advised to use a deep neural network (DNN) model instead of the previous prediction model for this enhanced dataset, specifically a one-dimensional convolutional neural network (1D-CNN), which is well suited for training this reconstruction data through this experiment.
Combining Convolutional Neural Network with Physics- Based Features in Shallow and Intermediate-depth Earthquake Discrimination
Authors: Zheng Zhou , Lin Binhua , Jin Xing , Kang Lanchi , Wang Shicheng , Zhou ShiWen , Zhou Yueyong , Wei Yongxiang , Li Shuilong , YU WeiHeng , Guo Yang
Published: October 2023

It is crucial for earthquake early warning (EEW) to distinguish earthquakes of various focal depths accurately and reliably. However, this task is a significant challenge due to the difficulty in interpreting the underlying physical mechanisms of earthquakes of different focal depths. In this study, we proposed an algorithm that combines a convolutional neural network with physics parameter-based features (CNN-PP) to discriminate between shallow and intermediate-depth earthquakes. A total of 3586 earthquakes in Japan recorded by the K-NET and KiK-net strong-motion seismograph networks from 2003 to 2020 were collected and processed as research data; 38081 Three-channel acceleration seismic records were obtained by station record interception, baseline correction and quality screening along with other pre-processing procedures. Among them, 26644 and 11437 records were used as the training and the test dataset, respectively. The test results show that the CNN-PP model outperforms the CNN model in discriminating shallow and intermediate-depth earthquakes. In addition, we test the CNN-PP model with the seismic events (M ≥ 3) that occurred in Japan in February 2022, and the results confirmed that this model has good performance in discriminating earthquakes of varying magnitudes. The CNN-PP model can effectively discriminate shallow and intermediate-depth earthquakes and has great application potential in EEW.
Morphological and conductivity studies of fullerene and N-vinyl carbazole based interpenetrating Polymer network
Authors: Jafri Mohd. Meraj , Kamal Meet
Published: October 2023

In the present study we have synthesized interpenetrating polymer network (IPN) of fullerene and N-vinyl carbazole (NVK). The IPN was characterized using infrared (IR) spectroscopy, scanning electron microscope (SEM) and conductivity techniques which reveals desirable and interesting results. IR spectroscopy reveals presence of fullerene at 527 cm− 1 and 1600 cm− 1 and poly (N-vinyl carbazole) at 1101 cm− 1. A thin polymeric interconnected film is seen via SEM technique, revealing transparent and dual phase morphology. IPN was also analyzed for permeability and permittivity, which reveals maximum value of εr՛, εr՛՛ and tanδε at 2.77, 0.48 and 0.11 respectively and maximum values of µ՛r of µ՛՛r and tanµr ranging from 0.99 to 1.50, 0.01–0.103 and 0.01–0.08 respectively. Moderate values of permeability and permittivity reveals electromagnetic property of the IPN. Conductivity IPN was calculated and found to be was found to be 4.98 S/m which describes the semi conducting nature of the polymeric network.
Targeted metabolic profiling in determining the metabolic heterogeneity in human biopsies of different grades of glioma
Authors: Vanisree Arambakkam Janardhanan , Gangadharan Thamizhoviya
Published: October 2023

Gliomas are intricate tumors with numerous metabolic and genetic abnormalities contributing to their aggressive phenotypes and poor prognoses. The study aims at identifying the key molecular metabolic as well as gene expressional variations that could be used not only to differentiate between different grades of glioma, with potential for improved early diagnostics but also to obtain a deeper insight about metabolic observation of glioma. In the present study, the metabolomic profiling along with clinical, and expressional analyses of glioma biopsies (n = 56; patients comprising both of benign and malignant lesions) were analyzed. The biopsies were subjected to gene/protein expressional analysis using RT-PCR, western blotting and also were subjected to metabolite analyses. The results of the gene/protein expressional analysis exhibited elevated levels of carnitine palmitoyltransferase, monoglyceride lipase, human phosphofructokinase, and isocitrate dehydrogenase in higher grades of glioma when compared to those of control. Our study suggested that the metabolites and gene/protein expressional levels were found to be discriminative among the grades of glioma. The study is deemed as a provider of deeper insights that are essential for differential therapeutic approaches that specifically target the dysregulated metabolome to the benefit of patients.
Free-Form Grid Structure Multi-Objective Optimization Associated with Integrated Design and Machine Learning
Authors: Fard Mahdi S. , Ghaderi Elham , Fooladi Mohammad Pour
Published: October 2023

Grid structures are among the lightest spatial structures for supporting large-span shells while constructing an efficient free-form casefree-form is exceedingly challenging. This research aims to investigate a special type of pattern for free-form grid structures using a process that combines selected design variables with their corresponding structural properties. Therefore, functional grid structures based on traditional Iranian geometries are parametrically interconnected in a workflow, where the results are compared to a base case. Iranian Chahar-Lengeh Girih as the main generative designer is generated by the Hankin Method that defines a controlling parameter (P) for the shape typology where the elements of the structure support a simple quadrilateral grid or a reciprocal one. Comparing these two validates the structural efficiency of architectural and structural performance. The workflow carries out a multi-objective evolutionary algorithm (NSGAII) to optimize the structural performance of the free-form grid structures with satisfying the architectural design requirements concomitantly. It is composed of the design-related part which includes the parameters of generating free-form grid structures, while the element diameter, element thickness, and the number of columns represents the structure-related counterparts. Consequently, reducing Mass, Displacement, and Elastic Energy are optimization objectives, besides K-Means Clustering with diverse approaches is used to explore patterns of possible solutions among the functions. The results portray that in the Overall-Best solution extracted by the workflow, with a P parameter equal to 0.8, Displacement, and Elastic Energy are improved by %88.44 and %89.82, respectively compared to its Reference Case where P = 0.5 (simple grid shell).
Pregnancy outcomes in patients complicated with pre-excitation syndrome
Authors: Wang Kana , Xin Junguo , Hu Qing , Wang Xiaodong , Yu Haiyan
Published: October 2023

Introduction:
Pregnant women with pre-excitation syndrome are more likely to develop supraventricular tachycardia (SVT) during pregnancy and delivery, leading to an increased risk of adverse events.
Method
This was a retrospective study of 309 pregnancies in 280 women (29 women had two pregnancies in this series) with pre-excitation syndrome who delivered at West China Second University Hospital from June 2011 to October 2021. All of the recruited pregnant women were divided into SVT and non-SVT groups to analyze the cardiac and obstetric complications.
Results
Among the included pregnant women, the prevalence of pre-excitation syndrome was 0.24% (309/127725). In the 309 cases with pre-excitation syndrome during pregnancy, 62 (20.1%, 62/309) had a history of SVT. In the 62 cases with SVT during pregnancy, 22 (35.5%) cases had a history of SVT. Gestational diabetes mellitus was associated with SVT during pregnancy. The cesarean section rate was 88.7% in the SVT group, which was significantly higher than that in the non-SVT group (64.8%) (P < 0.001). Cases with SVT during pregnancy had more cardiac and obstetric complications. Four fetal deaths were recorded in the SVT group. Additionally, 29 women experienced two pregnancies during the study period, among whom, five received radiofrequency ablation after the first delivery and obtained better outcomes in the second pregnancy.
Conclusion
The outcomes of pregnant women with pre-excitation syndrome were closely related to SVT, with possible risk factors including history of SVT before pregnancy, cardiac function, heart organic abnormalities, and gestational diabetes mellitus.
The impact of budget transparency on tax revenue forecasts: Evidence from Croatian local government units
Authors: Prijaković Simona
Published: October 2023

The tax revenues that are actually collected by local government units for a fiscal year frequently differ from what is planned in their enacted budgets. Despite the literature on budget credibility, little is known about the effect of budget transparency on budget forecasting deviations and errors. This paper analyses whether the level of online local budget transparency (OLBT) affects tax revenue forecasting and the impact of other control variables (e.g., budget balance, income, population, political ideology and strength) on budget deviations for all 556 Croatian local government units (LGUs; cities and municipalities) during the period 2016–2022. This paper also analyses public budget cycles in tax revenue planning at the Croatian local government level. The results show that in LGUs where OLBT increases in pre-election years, underestimation of tax revenues is higher, and overestimation is lower. In election and post-election years, with increased OLBT, underestimation of tax revenues is lower and overestimation is higher.
Adsorption and decomposition of water molecules on Al and/or Ga-doped Graphene at ambient temperature: density functional theory
Authors: Afshari Sadegh
Published: October 2023

To use H2 gas as a common fuel it needs to be in high pressures or cryogenic temperatures to have reasonable density. But, if we have adsorbent materials with high volumetric capacities to store hydrogen at ambient temperature and low pressure without any compressing it is worthwhile to use hydrogen as clean and reversible fuel. Here, we want to report the adsorption and decomposition properties of aluminum and gallium-doped graphene at ambient temperature. We studied the adsorption of H2O molecule on pure and doped graphene via density functional theory. So, possible interactions between the H2O molecule from three sides and pure and aluminum and gallium-doped were examined. After adsorption, decomposition of the H2O molecule has been studied and so on, for receive a reaction pathway, possible intermediates and transition states has been studied. To continue the density of states, interaction energies and thermodynamic parameters have been calculated. The results showed that the adsorbed water on aluminum and/or gallium-doped graphene decompose to OH and H and then adsorb on the surface again at ambient temperature and this process was thermodynamically favorable.
Experience of symptom clusters and the supportive needs of patients undergoing concurrent chemoradiotherapy for cervical cancer: a qualitative study
Authors: Song Zihe , Jiang Jinxia , duan xia , yan xiaoxue
Published: October 2023

Purpose
To explore the experience of symptom clusters and the current status of supportive need of cervical cancer patients after concurrent chemoradiotherapy and to provide a basis for improving symptom management and social support systems for cervical cancer patients.
Methods
In this phenomenological study, a total of 13 patients who had undergone concurrent chemoradiotherapy for cervical cancer were selected using a purposive sampling method. From January to October 2022, semi-structured face-to-face interviews were conducted to collect data and a seven-step Colaizzi process was used for data analysis.
Results
Three themes for the symptom cluster experience were found: declining quality of life, prominent negative emotions, and ineffective response to symptoms. Three themes of patient support needs were identified: mental and psychological needs, medical service needs and desired social support.The subtopics corresponded to these themes.
Conclusions
Patients with concurrent chemoradiotherapy of cervical cancer have a long disease course, more adverse reactions, and many cluster symptoms, which lead to a high demand for support. Medical staff should strengthen the education of patients about the disease, provide a systematic continuity of care information management platform, establish an effective emotional support system, make comprehensive efforts to reduce the financial burden on patients, promote physical and mental rehabilitation of patients, and improve their quality of life.
Short variable regions flaA gene (SVR-flaA) diversity of multidrug resistant Campylobacter strains from poultry and poultry meat in India
Authors: Iqbal Saima , Qureshi Sabia , Banday Muddasir. S , Farooq Shaheen , Kashoo Zahid A , Gulzar Maliha , Bhat M. Altaf , Pandit Arif , Hussain Md. Isfaqul , Dar Pervaiz , Badroo Gulzar , Hafiz Mahrukh , din Faheem ud , Mehraj Junaid
Published: October 2023

Human gastrointestinal infections caused byCampylobacterspecies is the second most important foodborne illness after Salmonellosis worldwide. Poultry are the largest reservoir forCampylobacterorganisms. In the present study the short variable region of flagellin gene (SVR-flaA) typing was carried out to determine the variation among the circulating strains ofC. jejuniandC. coli. TheCampylobacter jejuniandC coliisolated from poultry birds and poultry meat were screened for the presence of virulence determinants likecadF,flaA,cdtB, andwlaN gene. The screening forwlaN gene is crucial in view of fact thatC jejunistrains that elicit Guillian Barre’s (GB) syndrome in humans carrywlaN gene. Out of the 200 samples comprising of poultry meat and cloacal swabs, 21.5% of samples were found positive forCampylobacterspp, 2.5% forC. jejuni, and 19% forC. coli. ThecadF,flaA,cdtB virulence genes were detected in all strains ofCampylobacterisolated in the present study. The presence of thewlaN gene in theC jejunistrains isolated in the present study may pose a public health threat with long term human health implications. The SVR-flaA typing ofCampylobacterstrains revealed thatCampylobacter coli flaA sequence 0L471375 is a new strain with a novel allele type 1675 and peptide sequence 5 which stands deposited in pubMLST database forCampylobacter. The otherflaA-SVR gene sequences identified in this study were OL471369, OL471370, OL471371, OL471372, OL471373, and OL471374. VariableDdeI-RFLP patterns ofCampylobacterstrains in the present study ranging from 100-1000bp were observed. Antimicrobial profiling of the strains revealed 100% resistance to ciprofloxacin (CIP), ampicillin (AMP), penicillin (PCN) nalidixic acid (NAL) whereas resistance to Tetracycline (TET) was 57.1%, 57.1% for erythromycin (Ery) 28% for amoxicillin (AMX) and enrofloxacin (ENFX) and 85% for amikacin (AMK). The high degree of resistance to fluoroquinoles is important in view of fluoroquinolones being drugs of choice for treatment of humanCampylobacterinfections.
WITHDRAWN: Cytotoxicity and wound healing efficacy of biosynthesis ZnO Nanoparticles Using Aspergillus niger against Multidrug Resistant Pseudomonas aeruginosa
Authors: Moustafa Elham E. , Hewedy Maha A. , Abdelbary Salah , Mohammed Howida R. , Kheiralla Zeinab M.H.
Published: October 2023

Background
Critically ill patients, particularly burn patients, are at substantial risk from Pseudomonas aeruginosa infection, which is a common source of healthcare-acquired infections. The rise of multidrug resistant pathogens is increasing, making control difficult. Infectious infections continue to be a public health concern worldwide, owing to growing resistance to antimicrobial agents. The current study was designed to assess the effectiveness of biologically produced zinc oxide nanoparticles (ZnO-NPs) in both vitro and vivo against MDR P. aeruginosa.
Results
Four soil samples were gathered from various sites. Fifteen fungus isolates were identified and tested for their ability to produce green ZnO NPs. The antibacterial activity of the biosynthesized ZnO NPs was evaluated against P. aeruginosa. Using morphological and molecular methods, a powerful fungal isolate in producing ZnO NPs was identified. The isolate of the fungus was recognized as A. niger. ZnO-NPs were produced biologically and UV-vis spectroscopy, Fourier-transform infrared (FT-IR), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD), and Zeta potential and particle size analysis were then used to characterize the samples. The biosynthesized ZnO NPs had no impact on normal melanocyte mammalian cell line (Hfb4) with IC50 365.64µM, had an antitumor effect on breast cancer cell lines (Mcf7) with IC50 61.92µM and displayed antiviral effects against the herpes simplex virus type 1 (Hvs1) (91.4%) at 250 µg/ml. Using female albino rats infected with P. aeruginosa, the antibacterial efficacy and wound healing effectiveness of synthesized ZnO NPs were assessed and their activity was compared with different agents including saline solution alone, fusidic acid (20 ml/g), and chemically synthesized ZnO NP. In comparison to other agents employed, the findings demonstrated that biosynthesized ZnO NPs have a significant impact on the process of wound healing. An estimation of the oxidative stress and immune response in rats given biosynthesized ZnO NP treatments revealed that oxidative stress and inflammation were reduced because of the presence of infection. Three weeks later, histopathological examination was performed to assess the wound healing development.
Conclusions
The outcomes showed after 14 days, ZnO NPs are highly effective against MDR P. aeruginosa and accelerate the healing of wounds.
Nanoplastics induces Arrhythmia in Human Stem-cells derived Cardiomyocytes
Authors: Chia Shirley Pei Shan , Pang Jeremy Kah Sheng , Soh Boon-Seng
Published: October 2023

Plastic has become an ubiquitous environmental pollutant and nanoplastics (NPs) that are within the size range of 1nm to 1000nm could form upon weathering. Considering its sheer size, NPs are speculated to be more hazardous than their larger counterparts. Despite the growing concern, there is still limited understanding on the effects of NPs on human heart. Therefore, we aim to utilise human embryonic stem cells-derived-cardiomyocytes (hESC-CMs) to investigate the effects related to the uptake and accumulation of NPs in human heart. Firstly, more mature CMs were generated to better recapitulate the effects of NPs in adulthood. NPs effects were then elucidated over 3, 5 and 7 days of NPs treatment. The size-dependent uptake and accumulation of NPs was then established in CMs. Generally, oxidative stress and endoplasmic reticulum stress was upregulated in CMs in a dose-dependent manner. On the other hand, a rise in apoptosis was noted on all timepoints and was significant on day 7. Correspondingly, arrhythmia was also induced by day 7 of NPs treatment. Overall, our findings suggested that an exposure and accumulation of nanoplastics within hESC-CMs leads to oxidative stress, endoplasmic reticulum stress and decreased cell viability, resulting in an arrhythmic phenotype.
Resveratrol Safeguards Pre-osteoblast MC3T3-E1 Cells by Fostering Cell Growth and Specialization while Restraining Apoptosis
Authors: He Yu , Liu Fei , He Mingjuan , Long Fayu , Hu Ding , Chen Jingwen , Fang Miao , Wang Zhenlong
Published: October 2023

The purpose of this study was to investigate the mechanism by which resveratrol (Res) inhibits apoptosis and promotes proliferation and differentiation of pre-osteoblastic MC3T3-E1 cells, laying the groundwork for the treatment of osteoporosis (OP). The TCMSP database was used to find the gene targets for Res. The GeneCards database acquire the gene targets for OP. After discovering the potential target genes, GO, KEGG, and Reactome enrichment analysis were conducted. Verifying the major proteins involved in apoptosis can bind to Res using molecular docking. CCK8 measured the proliferative activity of mouse pre-osteoblasts in every group following Res intervention. Alkaline phosphatase staining (ALP) and alizarin red staining to measure the ability of osteogenic differentiation. RT-qPCR to determine the expression levels of Runx2 and OPG genes for osteogenic differentiation ability of cells. Western blot to measure the degree of apoptosis-related protein activity in each group following Res intervention. The biological processes investigated for GO of Res therapeutic OP involved in cytokine-mediated signaling pathway, negative regulation of apoptotic process, Aging, extrinsic apoptotic signaling pathway in absence of ligand, according to potential therapeutic target enrichment study. Apoptosis, FoxO signaling pathway, and TNF signaling pathway are the primary KEGG signaling pathways. Recactome pathways are primarily engaged in Programmed Cell Death, Apoptosis, Intrinsic Apoptotic Pathway, and Caspase activation via extrinsic apoptotic signaling pathways. This research established a new approach for Res treatment of OP by demonstrating how Res controls the apoptosis-related proteins TNF, IL6, and CASP3 to suppress osteoblast death and increase osteoclastogenesis.
Personalized Medicine for Cardiovascular Disease Risk in Artificial Intelligence Framework
Authors: Singh Manasvi , Kumar Ashish , Khanna Narendra N. , Laird John R. , Nicolaides Andrew , Faa Gavino , Johri Amer M. , Mantella Laura E. , Fernandes Jose , Teji Jagjit S. , Singh Narpinder , Fouda Mostafa M. , Sharma Aditya , Kitas George , Rathore Vijay , Singh Inder M. , Tadepalli Kalyan , Al-Maini Mustafa , Isenovic Esma R. , Chaturvedi Seemant , Paraskevas Kosmas I. , Mikhailidis Dimitri P. , Viswanathan Vijay , Kalra Manudeep K , Ruzsa Zoltan , Saba Luca , Laine Andrew F. , Bhatt Deepak L. , Suri Jasjit S.
Published: October 2023

Background & Motivation:The field of personalized medicine endeavors to transform the healthcare industry by advancing individualized strategies for diagnosis, treatment modalities, and prognostic assessments. This is achieved by utilizing extensive multidimensional biological datasets encompassing diverse components, such as an individual's genetic makeup, functional attributes, and environmental influences. Medical practitioners can use this strategy to tailor early interventions for each patient's explicit treatment or preventative requirements. Artificial intelligence (AI) systems, namely machine learning (ML) and deep learning (DL), have exhibited remarkable efficacy in predicting the potential occurrence of specific cancers and cardiovascular diseases (CVD).Methods:In this comprehensive analysis, we conducted a detailed examination of the term "personalized medicine," delving into its fundamental principles, the obstacles it encounters as an emerging subject, and its potentially revolutionary implications in the domain of CVD. A total of 228 studies were selected using the PRISMA methodology.Findings and Conclusions: Herein, we provide a scoping review highlighting the role of AI, particularly DL, in personalized risk assessment for CVDs. It underscores the prospect for AI-driven personalized medicine to significantly improve the accuracy and efficiency of controlling CVD, revolutionizing patient outcomes. The article also presents examples from real-world case studies and outlines potential areas for future research.
Influence of Larval Growth and Habitat Shading on Retreatment Frequencies of Biolarvicides against Dominant Malaria Vectors in Tanzania
Authors: Msugupakulya Betwel J. , Ngajuma Swedi K. , Ngayambwa Athuman N. , Kidwanga Baraka E. , Mpasuka Ibrahim R. , Selvaraj Prashanth , Wilson Anne L. , Okumu Fredros O.
Published: October 2023

We investigated the efficacy of two biolarvicides, VectoBac® and VectoMax®, against Anopheles funestus and Anopheles arabiensis, in shaded and unshaded semi-natural habitats; and explored how their varied larval development durations might influence retreatment intervals. Semi-natural aquatic habitats were created for field-collected An. funestus and An. arabiensis larvae in sun-exposed and shaded locations and treated with VectoBac® or VectoMax® or left untreated as controls. The larval development was assessed in semi-natural and natural habitats by recording daily densities of larval and pupal stages. Both biolarvicides reduced densities of early and late instar larvae of both mosquito species by over 98% within 72 hours. Efficacy lasted one week in sun-exposed environments regardless of dose but remained above 50% for two weeks in shaded habitats. On average, An. funestus larvae spent up to two weeks before pupating (13.2[10.4–16.0] days in semi-natural and 10.0[6.6–13.5] days in natural habitats), while An. arabiensis pupated after slightly over one week (8.2 [5.8–10.6] days in semi-natural and 8.32 [5.0-11.6] days in natural habitats). This data suggests that An. funestus might need a minimum of biweekly retreatments, while An. arabiensis requires a minimum of weekly treatments. Moreover, sun-exposed habitats may need more frequent applications than shaded habitats.
Systematic Evaluation of Merkel Cell Carcinoma Clinical Practice Guidelines Using the AGREE II Instrument
Authors: Lakshmipathy Deepak , Fritz Christian , Harris Jacob , Athni Tejas , Go Beatrice , Moreira Alvaro , Giordano Cerrene , Rajasekaran Karthik
Published: October 2023

Merkel cell carcinoma (MCC) is a rare type of skin cancer that requires a multidisciplinary approach with a variety of specialists for management and treatment. Clinical practice guidelines (CPGs) have recently been established to standardize management algorithms. The objective of this study was to appraise such CPGs via the Appraisal of Guidelines for Research and Evaluation (AGREE II) instrument. Eight CPGs were identified via systematic literature search following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) criteria. Four appraisers trained in AGREE II protocols evaluated each CPG and deemed two CPGs as high quality, five as moderate quality, and one as low quality. Intraclass correlation coefficients (ICCs) were calculated to verify reviewer consistency as excellent, good, and moderate across four, one, and one domain, respectively. The majority of MCC CPGs are lacking in specifying stakeholder involvement, applicability, and rigor of development. The two high quality CPGs are from the Alberta Health Services (AHS) and the collaboration between the European Dermatology Forum, the European Association of Dermato-Oncology, and the European Organization of Research and Treatment of Cancer (EDF/EADO/EORTC). The EDF/EADO/EORTC CPG had the highest overall score with no significant deficiencies across any domain. An important limitation is that the AGREE II instrument is not designed to evaluate the validity of each CPG’s recommendations; conclusions therefore can only be drawn about each CPG’s developmental quality. Future MCC CPGs may benefit from garnering public perspectives, inviting external expert review, and considering available resources and implementation barriers during their developmental stages.