bluuebunny/crossref_metadata_2025 huggingface dataset:
In-hospital outcome of Fascicular block following ST elevated Acute Myocardial Infarction
Authors: Ali Md Ashraf , Alo Dilara , Uddin Mir Jamal
Published: April 2017

Objective: The aim of the study was to observe the magnitude of in-hospital morbidity and mortality in fascicular block following ST elevated acute myocardial infarction (AMI).Background: Fascicular block following ST elevated acute myocardial infarction is often seen in CCU. It predicts poorer in-hospital outcome and signifies underlying extensive myocardial damage with jeopardized conducting system.Materials and Methods: This prospective case control observational study was carried out among the S-T elevated AMI patients in the CCU of NICVD during the period of January 2004 to December 2004. One hundred consecutive patients of first attack of AMI with or without fascicular block were included in this study. The patients suffering from congenital heart disease, cardiomyopathies, valvular heart disease and the patients having permanent pacemaker or preexisting syndrome were excluded from the study. Fifty numbers of patients suffering newly diagnosed fascicular block with acute AMI was considered as case and equal number of patients without fascicular block was taken as control. Case selection was done with the help of history, physical examination, twelve leads surface ECG and echocardiography. In hospital outcome was observed in terms of morbidity and mortality. So, hospitalized patents were followed up daily both clinically and with bedside continuous ECG monitoring.Results: In- hospital mortality was 30% in AMI with fascicular block and 12% in AMI without fascicular block, the difference was statistically significant (p=0.027). In hospital morbidity was significantly higher (70%) in cases compared to control (40%). The relative risk indicates that in hospital complications were 2.97 times higher in patients complicated with fascicular block. The mean number of composite complications (CCF, complete heart block, cardiogenic shock, VT, VF, ejection fraction and haemodynamic status etc.) was 1.14+1.0 in comparison to. 64 .85 in control group and mean difference was statistically significant (P<0.001). The mean percentage of ejection fraction was 44.9+5.2 in case of study group and it was 48.4+ 4.3 in control group. Mortality among fascicular blocks with anterior AMI was highest (24%). It was highest (80%) in LBBB and lowest in RBBB (14.3%).Conclusion: Fascicular block following AMI was associated with higher complications and mortality rate. So the physicians should be more aware of the aggressive management of the patients with fascicular block found in AMI.KYAMC Journal Vol. 5, No.-1, Jul 2014, Page 467-471
Wilson's Disease
Authors: Hossain Md Alamgir , Hasan Rashidul
Published: April 2017

Introduction: Wilson's Disease referred to as hepatolenticular degeneration with excessive copper retention involving liver and brain. Hepatic manifestations predominate in children and neuropsychiatric manifestations predominate in adolescents, adults.Case presentation: This is a case report of 24 years old female with long standing difficulty in walking and speech. Computerized tomography imaging showed bilateral symmetrical hypodensity in lentiform nucleus of basal ganglia, thalamus due to copper deposition and slit lamp examination showed Kayser-Fleischer (KF) ring surrounding limbus corneae.Conclusion: Professional counselling and support in addition to drug treatment provide help to patient and her family to overcome their problems and improve the treatment outcome.KYAMC Journal Vol. 5, No.-1, Jul 2014, Page 488-490
The Dynamics of Frame-bridging: Exploring the Nuclear Discourse in Scotland
Authors: Brown Antje
Published: April 2017

Simple indicative factors such as political populism and resource abundance cannot fully explain the Scottish Government's anti-nuclear energy policy. To grasp the current policy stance, it is necessary to pay attention to the wider contextualisation of policy framing and specifically the dynamic of story-telling and frame-bridging that ultimately feeds into governmental policy. The Scottish Government's decisive ‘no’ to a new nuclear fleet can be better understood by considering the underlying (and deliberate) bridging of policy frames that is noticeable between environmental, pacifist, and Scottish independence actors. This bridging not only affects the individual sets of story-telling but also develops a dynamic that reinforces individual stories and transcends well beyond the groups' original remit and objectives. With the help of policy framing analysis, research interviews and documentary analysis, the article explores the dynamic connections between anti-nuclear and independence activists and their causes in Scotland. The article highlights their triangular bridges in terms of personnel, language and story-telling and argues that these are instrumental in shaping the Scottish Government's anti-nuclear energy policy.
PTMD Control on a Benchmark TV Tower under Earthquake and Wind Load Excitations
Authors: Lin Wei , Song Gangbing , Chen Shanghong
Published: April 2017

A pounding tuned mass damper (PTMD) is introduced by making use of the energy dissipated during impact. In the proposed PTMD, a viscoelastic layer is attached to an impact limitation collar so that energy can be further consumed and transferred to heat energy. An improved numerical model to simulate pounding force is proposed and verified through experimentation. The accuracy of the proposed model was validated against a traditional Hertz-based pounding model. A comparison showed that the improved model tends to have a better prediction of the peak pounding force. A simulation was then carried out by taking the benchmark Canton Tower, which is a super-tall structure, as the host structure. The dynamic responses of uncontrolled, TMD-controlled and PTMD controlled system were simulated under wind and earthquake excitations. Unlike traditional TMDs, which are sensitive to input excitations and the mass ratio, the proposed PTMD maintains a stable level of control efficiency when the structure is excited by different earthquake records and different intensities. Particularly, more improvement can be observed when an extreme earthquake is considered. The proposed PTMD was able to achieve similar, or even better, control effectiveness with a lower mass ratio. These results demonstrate the superior adaptability of the PTMD and its applicability for protection of a building against seismic activity. A parametric study was then performed to investigate the influence of the mass ratio and the gap value on the control efficiency. A comparison of results show that better control results will be guaranteed by optimization of the gap value.
Low Complexity List Decoding for Polar Codes with Multiple CRC Codes
Authors: Kim Jong-Hwan , Kim Sang-Hyo , Jang Ji-Woong , Kim Young-Sik
Published: April 2017

Polar codes are the first family of error correcting codes that provably achieve the capacity of symmetric binary-input discrete memoryless channels with low complexity. Since the development of polar codes, there have been many studies to improve their finite-length performance. As a result, polar codes are now adopted as a channel code for the control channel of 5G new radio of the 3rd generation partnership project. However, the decoder implementation is one of the big practical problems and low complexity decoding has been studied. This paper addresses a low complexity successive cancellation list decoding for polar codes utilizing multiple cyclic redundancy check (CRC) codes. While some research uses multiple CRC codes to reduce memory and time complexity, we consider the operational complexity of decoding, and reduce it by optimizing CRC positions in combination with a modified decoding operation. Resultingly, the proposed scheme obtains not only complexity reduction from early stopping of decoding, but also additional reduction from the reduced number of decoding paths.
Automatic Tracking of the Modal Parameters of an Offshore Wind Turbine Drivetrain System
Authors: El-Kafafy Mahmoud , Devriendt Christof , Guillaume Patrick , Helsen Jan
Published: April 2017

An offshore wind turbine (OWT) is a complex structure that consists of different parts (e.g., foundation, tower, drivetrain, blades, et al.). The last decade, there has been continuous trend towards larger machines with the goal of cost reduction. Modal behavior is an important design aspect. For tackling noise, vibration, and harshness (NVH) issues and validating complex simulation models, it is of high interest to continuously track the vibration levels and the evolution of the modal parameters (resonance frequencies, damping ratios, mode shapes) of the fundamental modes of the turbine. Wind turbines are multi-physical machines with significant interaction between their subcomponents. This paper will present the possibility of identifying and automatically tracking the structural vibration modes of the drivetrain system of an instrumented OWT by using signals (e.g., acceleration responses) measured on the drivetrain system. The experimental data has been obtained during a measurement campaign on an OWT in the Belgian North Sea where the OWT was in standstill condition. The drivetrain, more specifically the gearbox and generator, is instrumented with a dedicated measurement set-up consisting of 17 sensor channels with the aim to continuously track the vibration modes. The consistency of modal parameter estimates made at consequent 10-min intervals is validated, and the dominant drivetrain modal behavior is identified and automatically tracked.
Enhancing Oil Recovery from Chalk Reservoirs by a Low-Salinity Water Flooding Mechanism and Fluid/Rock Interactions
Authors: Hamouda Aly A. , Gupta Sachin
Published: April 2017

Different Low Salinity Waters (LSWs) are investigated in this work to understand the role of some ions, which were recognized from our previous work and the literature for their effect on wettability alteration. Different flooding stages were followed. The primary stage was by injecting synthetic seawater (SSW) and the secondary stage was with SSW diluted by 10 (LSW 1:10) and 50 (LSW 1:50) times, single and two salt brines, such as Na2SO4, MgCl2, and NaCl+MgCl2 at 70 °C. The flooding sequence was due to that most of the fields in the North Sea were flooded with seawater. Two flooding rates were followed, 4 PV/day (PV = Pore Volume) and 16 PV/day in all the experiments. One of the observations was the increase of the pH during the flooding with LSW and single salt brines. The increase of the pH was attributed to mineral precipitation/dissolution as the results of ionic interactions. The effluent ion concentrations measured to understand the most likely oil recovery mechanisms. The results showed that the higher the SSW dilution the slower the oil recovery response. In presence of SO42−, Ca/Mg, higher oil recovery. The exchange between Ca/Mg, was in line with field observations. A geochemical simulation was done for a comparison with the experimental data.
Determination of Water Quality Degradation Due to Industrial and Household Wastewater in the Galing River in Kuantan, Malaysia Using Ion Chromatograph and Water Quality Data
Authors: Kozaki Daisuke , Harun Norhasmira , Rahim Mohd , Mori Masanobu , Nakatani Nobutake , Tanaka Kazuhiko
Published: April 2017

Water quality of the Galing River in Kuantan, Malaysia was examined to understand the anthropogenic environmental load in each administrative section, using water quality monitoring data and land use pattern. The National Physical Plan 2005 identified Kuantan as one of the country’s future growth centers, which has resulted in rapid development and environmental degradation in the past decade. Multiple water quality indexes used by the Department of Environment, Malaysia and concentrations of several ionic species were examined to assess the river’s water quality. The following inferences were drawn in this study: (1) Cl− and Na+ concentrations indicated that the basin area near the eastern urbanized area was subject to lesser human influence and lower environmental burden; (2) the Western side of the Galing River was subject to higher anthropogenic influence and indicated lower class levels of ammoniacal nitrogen, chemical oxygen demand, and dissolved oxygen, compared to the eastern side; (3) Class V or near class V pH values were obtained upstream at the western side of the Galing River in the industrial area; (4) Two types of environmental burden were identified in the western side of the Galing River, namely, inflow of industrial wastewater upstream on the western side and the effect of household wastewater or untreated raw sewage wastewater.
Assessing Impacts of Wood Utilisation Scenarios for a Lithuanian Bioeconomy: Impacts on Carbon in Forests and Harvested Wood Products and on the Socio-Economic Performance of the Forest-Based Sector
Authors: Jasinevičius Gediminas , Lindner Marcus , Verkerk Pieter , Aleinikovas Marius
Published: April 2017

Climate change and transition towards a bioeconomy are seen as both challenges and opportunities for the forest-based sector in Europe. Transition towards a bioeconomy will in most cases rely on intensified use of renewable resources and/or advancement in technology. However, how can the intensified use of renewable resources be combined with climate change mitigation measures to increase carbon sinks in the forest-based sector? Additionally, what are the possible socio-economic and environmental impacts of intensified wood use? In this study, we examined the impacts of increased wood utilisation in Lithuania. The objective of this study was to assess the effects of increased domestic wood utilisation on: (i) employment; (ii) the economic performance of the sector; (iii) carbon in forest biomass and soil; and (iv) carbon in harvested wood products (HWP). The system boundaries were set in accordance with international greenhouse gas reporting to include only domestic wood flows. We assessed alternative wood utilisation scenarios using a forest resource model and a tool to assess sustainability impacts of (wood) value chains, using country specific data on wood (carbon) flows. Our results indicate that increased wood use could lead to trade-offs between six selected indicators. Opportunities for employment and the economic performance of the forest-based sector improved in all scenarios due to increased wood utilisation. However, when forest fellings increased, the carbon stored in forests decreased, the carbon stored in HWP increased, but overall the total carbon stored in forests and HWP decreased. When considering also additional substitution effects until the year 2100, the scenario with reduced wood exports generated larger total climate change mitigation effects than the baseline. Our results suggest that increased wood utilisation might support Lithuania’s bioeconomy through increased socio-economic benefits. National positive climate change mitigation effects could be gained only if additional actions to utilise more domestic wood for long-life HWP will be taken.
Screening of Date Palm (Phoenix dactylifera L.) Cultivars for Salinity Tolerance
Authors: Al Kharusi Latifa , Assaha Dekoum , Al-Yahyai Rashid , Yaish Mahmoud
Published: April 2017

Date palm (Phoenix dactylifera L.) is a major fruit tree in the Middle East and it is a plant considered to be tolerant to a variety of abiotic stresses, including salinity. However, the physiological basis of its salinity tolerance is not fully known. The objective of this study was to screen Omani date palm cultivars for tolerance or susceptibility to salt stress. Seedlings from 10 commercially important date palm cultivars were subjected to 240 mM NaCl, and several physiological parameters related to salinity tolerance traits were evaluated upon treatment. The cultivars were divided into two groups based on the dry weight (DW) of their leaf and root tissues, a parameter which was used as an indication of healthy growth. The results revealed that photosynthesis, electrolyte leakage (EL), and the shoot K+/Na+ ratio were all significantly reduced in the susceptible cultivars. In addition, the relative water content was higher in the tolerant cultivars in comparison with the susceptible ones. These results suggest that although date palm is tolerant to high salinity, there is variation in tolerance among different cultivars. Shoot Na+ exclusion, photosynthesis, and membrane stability are apparently the main determinants of tolerance and can be used in salinity tolerance screening of date palm. The results have shown new very tolerant cultivars (Manoma and Umsila) that could serve as genetic resources for improved date palm tolerance to salinity.
Flow Configurations in a Y Splitting-Junction Microchannel
Authors: Sinibaldi Giorgia , Romano Giovanni
Published: April 2017

In the present work, the flow field in a splitting-junction micro channel with a Y shape, which is the simplest geometry to be employed for heat and mass transfer in micro-devices such as micro-heat-exchangers and micro-mixers, is investigated experimentally using micro Particle Image Velocimetry (μPIV). The angular divergence in the Y splitting is changed, as well as the Reynolds number, in order to investigate the instantaneous and mean flow fields to determine which configurations are more suitable for practical applications. The results show that the flow configuration is strongly dependent on the Y shape angle, especially in the junction part, and that there is also a significant dependence on the Reynolds number.
Synaptic Homeostasis and Its Immunological Disturbance in Neuromuscular Junction Disorders
Authors: Takamori Masaharu
Published: April 2017

In the neuromuscular junction, postsynaptic nicotinic acetylcholine receptor (nAChR) clustering, trans-synaptic communication and synaptic stabilization are modulated by the molecular mechanisms underlying synaptic plasticity. The synaptic functions are based presynaptically on the active zone architecture, synaptic vesicle proteins, Ca2+ channels and synaptic vesicle recycling. Postsynaptically, they are based on rapsyn-anchored nAChR clusters, localized sensitivity to ACh, and synaptic stabilization via linkage to the extracellular matrix so as to be precisely opposed to the nerve terminal. Focusing on neural agrin, Wnts, muscle-specific tyrosine kinase (a mediator of agrin and Wnts signalings and regulator of trans-synaptic communication), low-density lipoprotein receptor-related protein 4 (the receptor of agrin and Wnts and participant in retrograde signaling), laminin-network (including muscle-derived agrin), extracellular matrix proteins (participating in the synaptic stabilization) and presynaptic receptors (including muscarinic and adenosine receptors), we review the functional structures of the synapse by making reference to immunological pathogenecities in postsynaptic disease, myasthenia gravis. The synapse-related proteins including cortactin, coronin-6, caveolin-3, doublecortin, R-spondin 2, amyloid precursor family proteins, glia cell-derived neurotrophic factor and neurexins are also discussed in terms of their possible contribution to efficient synaptic transmission at the neuromuscular junction.
Glyoxalase Goes Green: The Expanding Roles of Glyoxalase in Plants
Authors: Sankaranarayanan Subramanian , Jamshed Muhammad , Kumar Abhinandan , Skori Logan , Scandola Sabine , Wang Tina , Spiegel David , Samuel Marcus
Published: April 2017

The ubiquitous glyoxalase enzymatic pathway is involved in the detoxification of methylglyoxal (MG), a cytotoxic byproduct of glycolysis. The glyoxalase system has been more extensively studied in animals versus plants. Plant glyoxalases have been primarily associated with stress responses and their overexpression is known to impart tolerance to various abiotic stresses. In plants, glyoxalases exist as multigene families, and new roles for glyoxalases in various developmental and signaling pathways have started to emerge. Glyoxalase-based MG detoxification has now been shown to be important for pollination responses. During self-incompatibility response in Brassicaceae, MG is required to target compatibility factors for proteasomal degradation, while accumulation of glyoxalase leads to MG detoxification and efficient pollination. In this review, we discuss the importance of glyoxalase systems and their emerging biological roles in plants.
A Risk Management Framework for Cloud Migration Decision Support
Authors: Islam Shareeful , Fenz Stefan , Weippl Edgar , Mouratidis Haralambos
Published: April 2017

Keywords: risk management framework; risk assessment; cloud migration; security; analytic hierarchy process (AHP); business value
Special Issue: Materials for Electrochemical Capacitors and Batteries
Authors: Wang Jian-Gan , Wei Bingqing
Published: April 2017

Electrochemical capacitors and rechargeable batteries have received worldwide attention due to their excellent energy storage capability for a variety of applications. The rapid development of these technologies is propelled by the advanced electrode materials and new energy storage systems. It is believed that research efforts can improve the device performance to meet the ever-increasing requirements of high energy density, high power density and long cycle life. This Special Issue aims to provide readers with a glimpse of different kinds of electrode materials for electrochemical capacitors and batteries.
Corrosion-Fatigue Crack Growth in Plates: A Model Based on the Paris Law
Authors: Toribio Jesús , Matos Juan-Carlos , González Beatriz
Published: April 2017

In this paper, a Paris law-based model is presented whereby crack propagation occurs under cyclic loading in air (fatigue) and in an aggressive environment (corrosion-fatigue) for the case of corner cracks (with a wide range of aspect ratios in the matter of the initial cracks) in finite-thickness plates of 316L austenitic stainless steel subjected to tension, bending, or combined (tension + bending) loading. Results show that the cracks tend during their growth towards a preferential propagation path, exhibiting aspect ratios slightly lower than unity only for the case of very shallow cracks, and diminishing as the crack grows (increasing the relative crack depth)—more intensely in the case of bending than in the case of tension (the mixed loading tension/bending representing an intermediate case). In addition, the crack aspect ratios during fatigue propagation evolution are lower in fatigue (in air) than in corrosion-fatigue (in aggressive environment).
Preparation of Porous Poly(Styrene-Divinylbenzene) Microspheres and Their Modification with Diazoresin for Mix-Mode HPLC Separations
Authors: Yu Bing , Xu Tao , Cong Hailin , Peng Qiaohong , Usman Muhammad
Published: April 2017

By using the two-step activated swelling method, monodisperse porous poly(styrene-divinylbenzene) (P(S-DVB)) microparticles were successfully synthesized. The influence of porogens, swelling temperatures and crosslinking agents on the porosity of porous microparticles was carefully investigated. Porous P(S-DVB) microparticles were used as a packing material for high performance liquid chromatography (HPLC). Several benzene analogues were effectively separated in a stainless-steel column as short as 75 mm due to the high specific surface area of the porous microparticles. Porous P(S-DVB) microparticles were further sulfonated and subsequently modified with diazoresin (DR) via electrostatic self-assembly and UV (ultraviolet) radiation. After treatment with UV light, the ionic bonding between sulfonated P(S-DVB) and DR was converted into covalent bonding through a unique photochemistry reaction of DR. Depending on the chemical structure of DR and mobile phase composition, the DR-modified P(S-DVB) stationary phase performed different separation mechanisms, including reversed phase (RP) and hydrophilic interactions. Therefore, baseline separations of benzene analogues and organic acids were achieved by using the DR-modified P(S-DVB) particles as packing materials in HPLC. According to the π–π interactional difference between carbon rings of fullerenes and benzene rings of DR, C60 and C70 were also well separated in the HPLC column packed with DR-modified P(S-DVB) particles.
Linear Graphene Nanocomposite Synthesis and an Analytical Application for the Amino Acid Detection of Camellia nitidissima Chi Seeds
Authors: Cheng Jinsheng , Zhong Ruimin , Lin Jiajian , Zhu Jianhua , Wan Weihong , Chen Xinyan
Published: April 2017

Husk derived amino modified linear graphene nanocomposites (aLGN) with a diameter range of 80–300 nm and a length range of 100–300 μm were prepared by a modified Hummers method, ammonia treatment, NaBH4 reduction and phenylalanine induced assembly processes, etc. The resulting composites were characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), scanning electron microscopy (SEM), biological microscope (BM), and X-ray diffraction spectroscopy (XRD), etc. Investigations found that the aLGN can serve as the novel coating of stir bar sorptive extraction (SBSE) technology. By combing this technology with gas chromatography–mass spectrometry (GC-MS), the combined SBSE/GC-MS technology with an aLGN coating can detect seventeen kinds of amino acids of Camellia nitidissima Chi seeds, including Ala, Gly, Thr, Ser, Val, Leu, Ile, Cys, Pro, Met, Asp, Phe, Glu, Lys, Tyr, His, and Arg. Compared to a conventional polydimethylsiloxane (PDMS) coating, an aLGN coating for SBSE exhibited a better thermal desorption performance, better analytes fragmentation depressing efficiencies, higher peak intensities, and superior amino acid discrimination, leading to a practicable and highly distinguishable method for the variable amino acid detection of Camellia nitidissima Chi seeds.
Reduced Fracture Toughness of Metallic Glass at Cryogenic Temperature
Authors: Zhou Yihao , Liu Jiaxin , Han Dongxue , Chen Xiangru , Wang Gang , Zhai Qijie
Published: April 2017

The effects of cryogenic temperature on the toughness of a Zr-based metallic glass are investigated. Based on three-dimensional fracture morphologies at different temperatures, the crack formation and propagation are analyzed. Through the calculation of the shear transformation zone volume, the shear modulus and bulk modulus of the metallic glass at different temperatures and the crack formation mechanism associated with the temperature is discussed. Once the crack commences propagation, the hyperelasticity model is used to elucidate the fractographic evolution of crack propagation.
Inhibitory Effect of Triterpenoids from Panax ginseng on Coagulation Factor X
Authors: Xiong Lingxin , Qi Zeng , Zheng Bingzhen , Li Zhuo , Wang Fang , Liu Jinping , Li Pingya
Published: April 2017

Enzymes involved in the coagulation process have received great attention as potential targets for the development of oral anti-coagulants. Among these enzymes, coagulation factor Xa (FXa) has remained the center of attention in the last decade. In this study, 16 ginsenosides and two sapogenins were isolated, identified and quantified. To determine the inhibitory potential on FXa, the chromogenic substrates method was used. The assay suggested that compounds 5, 13 and 18 were mainly responsible for the anti-coagulant effect. Furthermore, these three compounds also possessed high thrombin selectivity in the thrombin inhibition assay. Furthermore, Glide XP from Schrödinger was employed for molecular docking to clarify the interaction between the bioactive compounds and FXa. Therefore, the chemical and biological results indicate that compounds 5 (ginsenoside Rg2), 13 (ginsenoside Rg3) and 18 (protopanaxtriol, PPT) are potential natural inhibitors against FXa.
Phenolic Compounds Isolated from Caesalpinia coriaria Induce S and G2/M Phase Cell Cycle Arrest Differentially and Trigger Cell Death by Interfering with Microtubule Dynamics in Cancer Cell Lines
Authors: Sánchez-Carranza Jessica , Alvarez Laura , Marquina-Bahena Silvia , Salas-Vidal Enrique , Cuevas Verónica , Jiménez Elizabeth , Veloz G. Rafael , Carraz Maelle , González-Maya Leticia
Published: April 2017

Caesalpinia coriaria (C. coriaria), also named cascalote, has been known traditionally in México for having cicatrizing and inflammatory properties. Phytochemical reports on Caesalpinia species have identified a high content of phenolic compounds and shown antineoplastic effects against cancer cells. The aim of this study was to isolate and identify the active compounds of a water:acetone:ethanol (WAE) extract of C. coriaria pods and characterize their cytotoxic effect and cell death induction in different cancer cell lines. The compounds isolated and identified by chromatography and spectroscopic analysis were stigmasterol, ethyl gallate and gallic acid. Cytotoxic assays on cancer cells showed different ranges of activities. A differential effect on cell cycle progression was observed by flow cytometry. In particular, ethyl gallate and tannic acid induced G2/M phase cell cycle arrest and showed interesting effect on microtubule stabilization in Hep3B cells observed by immunofluorescence. The induction of apoptosis was characterized by morphological characteristic changes, and was supported by increases in the ratio of Bax/Bcl-2 expression and activation of caspase 3/7. This work constitutes the first phytochemical and cytotoxic study of C. coriaria and showed the action of its phenolic constituents on cell cycle, cell death and microtubules organization.
UVA, UVB and UVC Light Enhances the Biosynthesis of Phenolic Antioxidants in Fresh-Cut Carrot through a Synergistic Effect with Wounding
Authors: Surjadinata Bernadeth , Jacobo-Velázquez Daniel , Cisneros-Zevallos Luis
Published: April 2017

Previously, we found that phenolic content and antioxidant capacity (AOX) in carrots increased with wounding intensity. It was also reported that UV radiation may trigger the phenylpropanoid metabolism in plant tissues. Here, we determined the combined effect of wounding intensity and UV radiation on phenolic compounds, AOX, and the phenylalanine ammonia-lyase (PAL) activity of carrots. Accordingly, phenolic content, AOX, and PAL activity increased in cut carrots with the duration of UVC radiation, whereas whole carrots showed no increase. Carrot pies showed a higher increase compared to slices and shreds. Phenolics, AOX, and PAL activity also increased in cut carrots exposed to UVA or UVB. The major phenolics were chlorogenic acid and its isomers, ferulic acid, and isocoumarin. The type of UV radiation affected phenolic profiles. Chlorogenic acid was induced by all UV radiations but mostly by UVB and UVC, ferulic acid was induced by all UV lights to comparable levels, while isocoumarin and 4,5-diCQA was induced mainly by UVB and UVC compared to UVA. In general, total phenolics correlated linearly with AOX for all treatments. A reactive oxygen species (ROS) mediated hypothetical mechanism explaining the synergistic effect of wounding and different UV radiation stresses on phenolics accumulation in plants is herein proposed.
Polyphenolic Compounds and Digestive Enzymes: In Vitro Non-Covalent Interactions
Authors: Martinez-Gonzalez Alejandra I. , Díaz-Sánchez Ángel G. , Rosa Laura A. de la , Vargas-Requena Claudia L. , Bustos-Jaimes Ismael , Alvarez-Parrilla and Emilio
Published: April 2017

The digestive enzymes–polyphenolic compounds (PCs) interactions behind the inhibition of these enzymes have not been completely studied. The existing studies have mainly analyzed polyphenolic extracts and reported inhibition percentages of catalytic activities determined by UV-Vis spectroscopy techniques. Recently, pure PCs and new methods such as isothermal titration calorimetry and circular dichroism have been applied to describe these interactions. The present review focuses on PCs structural characteristics behind the inhibition of digestive enzymes, and progress of the used methods. Some characteristics such as molecular weight, number and position of substitution, and glycosylation of flavonoids seem to be related to the inhibitory effect of PCs; also, this effect seems to be different for carbohydrate-hydrolyzing enzymes and proteases. The digestive enzyme–PCs molecular interactions have shown that non-covalent binding, mostly by van der Waals forces, hydrogen binding, hydrophobic binding, and other electrostatic forces regulate them. These interactions were mainly associated to non-competitive type inhibitions of the enzymatic activities. The present review emphasizes on the digestive enzymes such as α-glycosidase (AG), α-amylase (PA), lipase (PL), pepsin (PE), trypsin (TP), and chymotrypsin (CT). Existing studies conducted in vitro allow one to elucidate the characteristics of the structure–function relationships, where differences between the structures of PCs might be the reason for different in vivo effects.
High-Performance Prediction of Human Estrogen Receptor Agonists Based on Chemical Structures
Authors: Asako Yuki , Uesawa Yoshihiro
Published: April 2017

Many agonists for the estrogen receptor are known to disrupt endocrine functioning. We have developed a computational model that predicts agonists for the estrogen receptor ligand-binding domain in an assay system. Our model was entered into the Tox21 Data Challenge 2014, a computational toxicology competition organized by the National Center for Advancing Translational Sciences. This competition aims to find high-performance predictive models for various adverse-outcome pathways, including the estrogen receptor. Our predictive model, which is based on the random forest method, delivered the best performance in its competition category. In the current study, the predictive performance of the random forest models was improved by strictly adjusting the hyperparameters to avoid overfitting. The random forest models were optimized from 4000 descriptors simultaneously applied to 10,000 activity assay results for the estrogen receptor ligand-binding domain, which have been measured and compiled by Tox21. Owing to the correlation between our model’s and the challenge’s results, we consider that our model currently possesses the highest predictive power on agonist activity of the estrogen receptor ligand-binding domain. Furthermore, analysis of the optimized model revealed some important features of the agonists, such as the number of hydroxyl groups in the molecules.
Adenosine A1 and A2A Receptors in the Brain: Current Research and Their Role in Neurodegeneration
Authors: Stockwell Jocelyn , Jakova Elisabet , Cayabyab Francisco
Published: April 2017

The inhibitory adenosine A1 receptor (A1R) and excitatory A2A receptor (A2AR) are predominantly expressed in the brain. Whereas the A2AR has been implicated in normal aging and enhancing neurotoxicity in multiple neurodegenerative diseases, the inhibitory A1R has traditionally been ascribed to have a neuroprotective function in various brain insults. This review provides a summary of the emerging role of prolonged A1R signaling and its potential cross-talk with A2AR in the cellular basis for increased neurotoxicity in neurodegenerative disorders. This A1R signaling enhances A2AR-mediated neurodegeneration, and provides a platform for future development of neuroprotective agents in stroke, Parkinson’s disease and epilepsy.
Structural and Biochemical Properties of Novel Self-Cleaving Ribozymes
Authors: Lee Ki-Young , Lee Bong-Jin
Published: April 2017

Fourteen well-defined ribozyme classes have been identified to date, among which nine are site-specific self-cleaving ribozymes. Very recently, small self-cleaving ribozymes have attracted renewed interest in their structure, biochemistry, and biological function since the discovery, during the last three years, of four novel ribozymes, termed twister, twister sister, pistol, and hatchet. In this review, we mainly address the structure, biochemistry, and catalytic mechanism of the novel ribozymes. They are characterized by distinct active site architectures and divergent, but similar, biochemical properties. The cleavage activities of the ribozymes are highly dependent upon divalent cations, pH, and base-specific mutations, which can cause changes in the nucleotide arrangement and/or electrostatic potential around the cleavage site. It is most likely that a guanine and adenine in close proximity of the cleavage site are involved in general acid-base catalysis. In addition, metal ions appear to play a structural rather than catalytic role although some of their crystal structures have shown a direct metal ion coordination to a non-bridging phosphate oxygen at the cleavage site. Collectively, the structural and biochemical data of the four newest ribozymes could contribute to advance our mechanistic understanding of how self-cleaving ribozymes accomplish their efficient site-specific RNA cleavages.
Feasibility of Using the Optical Sensing Techniques for Early Detection of Huanglongbing in Citrus Seedlings
Authors: Pourreza Alireza , Lee Won , Czarnecka Eva , Verner Lance , Gurley William
Published: April 2017

A vision sensor was introduced and tested for early detection of citrus Huanglongbing (HLB). This disease is caused by the bacterium Candidatus Liberibacter asiaticus (CLas) and is transmitted by the Asian citrus psyllid. HLB is a devastating disease that has exerted a significant impact on citrus yield and quality in Florida. Unfortunately, no cure has been reported for HLB. Starch accumulates in HLB infected leaf chloroplasts, which causes the mottled blotchy green pattern. Starch rotates the polarization plane of light. A polarized imaging technique was used to detect the polarization-rotation caused by the hyper-accumulation of starch as a pre-symptomatic indication of HLB in young seedlings. Citrus seedlings were grown in a room with controlled conditions and exposed to intensive feeding by CLas-positive psyllids for eight weeks. A quantitative polymerase chain reaction was employed to confirm the HLB status of samples. Two datasets were acquired; the first created one month after the exposer to psyllids and the second two months later. The results showed that, with relatively unsophisticated imaging equipment, four levels of HLB infections could be detected with accuracies of 72%–81%. As expected, increasing the time interval between psyllid exposure and imaging increased the development of symptoms and, accordingly, improved the detection accuracy.
Simulated Imagery Rendering Workflow for UAS-Based Photogrammetric 3D Reconstruction Accuracy Assessments
Authors: Slocum Richard , Parrish Christopher
Published: April 2017

Structure from motion (SfM) and MultiView Stereo (MVS) algorithms are increasingly being applied to imagery from unmanned aircraft systems (UAS) to generate point cloud data for various surveying and mapping applications. To date, the options for assessing the spatial accuracy of the SfM-MVS point clouds have primarily been limited to empirical accuracy assessments, which involve comparisons against reference data sets, which are both independent and of higher accuracy than the data they are being used to test. The acquisition of these reference data sets can be expensive, time consuming, and logistically challenging. Furthermore, these experiments are also almost always unable to be perfectly replicated and can contain numerous confounding variables, such as sun angle, cloud cover, wind, movement of objects in the scene, and camera thermal noise, to name a few. The combination of these factors leads to a situation in which robust, repeatable experiments are cost prohibitive, and the experiment results are frequently site-specific and condition-specific. Here, we present a workflow to render computer generated imagery using a virtual environment which can mimic the independent variables that would be experienced in a real-world UAS imagery acquisition scenario. The resultant modular workflow utilizes Blender, an open source computer graphics software, for the generation of photogrammetrically-accurate imagery suitable for SfM processing, with explicit control of camera interior orientation, exterior orientation, texture of objects in the scene, placement of objects in the scene, and ground control point (GCP) accuracy. The challenges and steps required to validate the photogrammetric accuracy of computer generated imagery are discussed, and an example experiment assessing accuracy of an SfM derived point cloud from imagery rendered using a computer graphics workflow is presented. The proposed workflow shows promise as a useful tool for sensitivity analysis and SfM-MVS experimentation.
The Use of IMMUs in a Water Environment: Instrument Validation and Application of 3D Multi-Body Kinematic Analysis in Medicine and Sport
Authors: Mangia Anna Lisa , Cortesi Matteo , Fantozzi Silvia , Giovanardi Andrea , Borra Davide , Gatta Giorgio
Published: April 2017

The aims of the present study were the instrumental validation of inertial-magnetic measurements units (IMMUs) in water, and the description of their use in clinical and sports aquatic applications applying customized 3D multi-body models. Firstly, several tests were performed to map the magnetic field in the swimming pool and to identify the best volume for experimental test acquisition with a mean dynamic orientation error lower than 5°. Successively, the gait and the swimming analyses were explored in terms of spatiotemporal and joint kinematics variables. The extraction of only spatiotemporal parameters highlighted several critical issues and the joint kinematic information has shown to be an added value for both rehabilitative and sport training purposes. Furthermore, 3D joint kinematics applied using the IMMUs provided similar quantitative information than that of more expensive and bulky systems but with a simpler and faster setup preparation, a lower time consuming processing phase, as well as the possibility to record and analyze a higher number of strides/strokes without limitations imposed by the cameras.
A Miniature Aerosol Sensor for Detecting Polydisperse Airborne Ultrafine Particles
Authors: Zhang Chao , Wang Dingqu , Zhu Rong , Yang Wenming , Jiang Peng
Published: April 2017

Counting and sizing of polydisperse airborne nanoparticles have attracted most attentions owing to increasing widespread presence of airborne engineered nanoparticles or ultrafine particles. Here we report a miniature aerosol sensor to detect particle size distribution of polydisperse ultrafine particles based on ion diffusion charging and electrical detection. The aerosol sensor comprises a couple of planar electrodes printed on two circuit boards assembled in parallel, where charging, precipitation and measurement sections are integrated into one chip, which can detect aerosol particle size in of 30–500 nm, number concentration in range of 5 × 102 – 5 × 107 /cm3. The average relative errors of the measured aerosol number concentration and the particle size are estimated to be 12.2% and 13.5% respectively. A novel measurement scheme is proposed to actualize a real-time detection of polydisperse particles by successively modulating the measurement voltage and deducing the particle size distribution through a smart data fusion algorithm. The effectiveness of the aerosol sensor is experimentally demonstrated via measurements of polystyrene latex (PSL) aerosol and nucleic acid aerosol, as well as sodium chloride aerosol particles.
Simulation Study of the Localization of a Near-Surface Crack Using an Air-Coupled Ultrasonic Sensor Array
Authors: Delrue Steven , Aleshin Vladislav , Sørensen Mikael , Lathauwer Lieven
Published: April 2017

The importance of Non-Destructive Testing (NDT) to check the integrity of materials in different fields of industry has increased significantly in recent years. Actually, industry demands NDT methods that allow fast (preferably non-contact) detection and localization of early-stage defects with easy-to-interpret results, so that even a non-expert field worker can carry out the testing. The main challenge is to combine as many of these requirements into one single technique. The concept of acoustic cameras, developed for low frequency NDT, meets most of the above-mentioned requirements. These cameras make use of an array of microphones to visualize noise sources by estimating the Direction Of Arrival (DOA) of the impinging sound waves. Until now, however, because of limitations in the frequency range and the lack of integrated nonlinear post-processing, acoustic camera systems have never been used for the localization of incipient damage. The goal of the current paper is to numerically investigate the capabilities of locating incipient damage by measuring the nonlinear airborne emission of the defect using a non-contact ultrasonic sensor array. We will consider a simple case of a sample with a single near-surface crack and prove that after efficient excitation of the defect sample, the nonlinear defect responses can be detected by a uniform linear sensor array. These responses are then used to determine the location of the defect by means of three different DOA algorithms. The results obtained in this study can be considered as a first step towards the development of a nonlinear ultrasonic camera system, comprising the ultrasonic sensor array as the hardware and nonlinear post-processing and source localization software.
Smartphone Location-Independent Physical Activity Recognition Based on Transportation Natural Vibration Analysis
Authors: Hur Taeho , Bang Jaehun , Kim Dohyeong , Banos Oresti , Lee Sungyoung
Published: April 2017

Activity recognition through smartphones has been proposed for a variety of applications. The orientation of the smartphone has a significant effect on the recognition accuracy; thus, researchers generally propose using features invariant to orientation or displacement to achieve this goal. However, those features reduce the capability of the recognition system to differentiate among some specific commuting activities (e.g., bus and subway) that normally involve similar postures. In this work, we recognize those activities by analyzing the vibrations of the vehicle in which the user is traveling. We extract natural vibration features of buses and subways to distinguish between them and address the confusion that can arise because the activities are both static in terms of user movement. We use the gyroscope to fix the accelerometer to the direction of gravity to achieve an orientation-free use of the sensor. We also propose a correction algorithm to increase the accuracy when used in free living conditions and a battery saving algorithm to consume less power without reducing performance. Our experimental results show that the proposed system can adequately recognize each activity, yielding better accuracy in the detection of bus and subway activities than existing methods.
Direction of Arrival Estimation for MIMO Radar via Unitary Nuclear Norm Minimization
Authors: Wang Xianpeng , Huang Mengxing , Wu Xiaoqin , Bi Guoan
Published: April 2017

In this paper, we consider the direction of arrival (DOA) estimation issue of noncircular (NC) source in multiple-input multiple-output (MIMO) radar and propose a novel unitary nuclear norm minimization (UNNM) algorithm. In the proposed method, the noncircular properties of signals are used to double the virtual array aperture, and the real-valued data are obtained by utilizing unitary transformation. Then a real-valued block sparse model is established based on a novel over-complete dictionary, and a UNNM algorithm is formulated for recovering the block-sparse matrix. In addition, the real-valued NC-MUSIC spectrum is used to design a weight matrix for reweighting the nuclear norm minimization to achieve the enhanced sparsity of solutions. Finally, the DOA is estimated by searching the non-zero blocks of the recovered matrix. Because of using the noncircular properties of signals to extend the virtual array aperture and an additional real structure to suppress the noise, the proposed method provides better performance compared with the conventional sparse recovery based algorithms. Furthermore, the proposed method can handle the case of underdetermined DOA estimation. Simulation results show the effectiveness and advantages of the proposed method.