bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhancing Gamma-Ray Burst Detection: Evaluation of Neural Network Background Estimator and Explainable AI Insights
Authors: Crupi Riccardo , Dilillo Giuseppe , Della Casa Giovanni , Fiore Fabrizio , Vacchi Andrea
Published: March 2024

The detection of Gamma-Ray Bursts (GRBs) using spaceborne X/gamma-ray photon detectors depends on a reliable background count rate estimate. This study focuses on evaluating a data-driven background estimator based on a neural network designed to adapt to various X/gamma-ray space telescopes. Three trials were conducted to assess the effectiveness and limitations of the proposed estimator. Firstly, quantile regression was employed to obtain an estimation with a confidence range prediction. Secondly, we assessed the performance of the neural network, emphasizing that a dataset of four months is sufficient for training. We tested its adaptability across various temporal contexts, identified its limitations and recommended re-training for each specific period. Thirdly, utilizing Explainable Artificial Intelligence (XAI) techniques, we delved into the neural network output, determining distinctions between a network trained during solar maxima and one trained during solar minima. This entails conducting a thorough analysis of the neural network behavior under varying solar conditions.
Optimizing the Indoor Air Quality in Historical Buildings: Strategies for Environmental Improvement and Public Health Enhancement
Authors: Piscitelli Prisco , Miani Alessandro , Mecca Saverio , Hodgton Rachel
Published: March 2024

The endeavor to maintain and enhance the indoor air quality (IAQ) in historical buildings transcends the traditional boundaries of cultural heritage preservation, emerging as a pivotal public health concern [...]
Inflammatory Bowel Diseases and Non-Alcoholic Fatty Liver Disease: Piecing a Complex Puzzle Together
Authors: Maresca Rossella , Mignini Irene , Varca Simone , Calvez Valentin , Termite Fabrizio , Esposto Giorgio , Laterza Lucrezia , Scaldaferri Franco , Ainora Maria Elena , Gasbarrini Antonio , Zocco Maria Assunta
Published: March 2024

Inflammatory bowel diseases (IBD), comprising Crohn’s disease and ulcerative colitis, are systemic and multifaceted disorders which affect other organs in addition to the gastrointestinal tract in up to 50% of cases. Extraintestinal manifestations may present before or after IBD diagnosis and negatively impact the intestinal disease course and patients’ quality of life, often requiring additional diagnostic evaluations or specific treatments. Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide. Current evidence shows an increased prevalence of NAFLD (and its more advanced stages, such as liver fibrosis and steatohepatitis) in IBD patients compared to the general population. Many different IBD-specific etiopathogenetic mechanisms have been hypothesized, including chronic inflammation, malabsorption, previous surgical interventions, changes in fecal microbiota, and drugs. However, the pathophysiological link between these two diseases is still poorly understood. In this review, we aim to provide a comprehensive overview of the potential mechanisms which have been investigated so far and highlight open issues still to be addressed for future studies.
Upregulation of Notch Signaling and Cell-Differentiation Inhibitory Transcription Factors in Stable Chronic Obstructive Pulmonary Disease Patients
Authors: Di Stefano Antonino , Gnemmi Isabella , Rosani Umberto , Maniscalco Mauro , D’Anna Silvestro Ennio , Brun Paola , Carriero Vitina , Bertolini Francesca , Balbi Bruno , Ricciardolo Fabio Luigi Massimo
Published: March 2024

Notch signaling is involved in the prevention of cell differentiation and cell fate in various organs, including the lungs. We aimed to determine the transcriptomic and protein expression of Notch receptors, their ligands, and related transcription factors in stable COPD. The expression and localization of Notch receptors, their ligands, and related transcription factors were measured in bronchial biopsies of individuals with stable mild/moderate (MCOPD) (n = 18) or severe/very severe (SCOPD) (n = 16) COPD, control smokers (CSs) (n = 13), and control nonsmokers (CNSs) (n = 11), and in the lung parenchyma of those with MCOPD (n = 13), CSs (n = 10), and CNSs (n = 10) using immunohistochemistry, ELISA tests, and transcriptome analyses. In the bronchial biopsies, Notch4 and HES7 significantly increased in the lamina propria of those with SCOPD compared to those with MCOPD, CSs, and CNSs. In the peripheral lung bronchiolar epithelium, Notch1 significantly increased in those with MCOPD and CSs compared to CNSs. ELISA tests of lung parenchyma homogenates showed significantly increased Notch2 in those with MCOPD compared to CSs and CNSs. Transcriptomic data in lung parenchyma showed increased DLL4 and HES1 mRNA levels in those with MCOPD and CSs compared to CNSs. These data show the increased expression of the Notch pathway in the lungs of those with stable COPD. These alterations may play a role in impairing the regenerative–reparative responses of diseased bronchioles and lung parenchyma.
Ti-Doped Co-Free Li1.2Mn0.6Ni0.2O2 Cathode Materials with Enhanced Electrochemical Performance for Lithium-Ion Batteries
Authors: Liu Sining , Yan Xin , Li Pengyu , Tian Xinru , Li Sinan , Tao Yunwen , Li Pengwei , Luo Shaohua
Published: March 2024

Cobalt-free manganese-based lithium-rich layered oxides (LLOs) have garnered research attention as prospective lithium-ion cathode materials owing to their large specific capacity and low price. However, their large-scale application is hindered by their low Coulombic efficiency, poor cycling performance, voltage attenuation, and structural phase transition. To address these issues, the LLO structure is modified via Ti doping at the manganese site herein. Ti-doped Li1.2Mn0.6−xTixNi0.2O2 (x = 0, 0.03, 0.05, 0.10, and 0.15) is prepared using the high-temperature solid-state method. The Ti-doped Li1.2Mn0.6Ni0.2O2 is calculated via first principles. The results show that Ti4+ doping improves the cycle stability and rate performance of Li1.2Mn0.6Ni0.2O2. Electrochemical test results show that the sample exhibits enhanced electrochemical performance when the Ti doping amount is 0.05. The discharge specific capacity at 0.1C is 210.4 mAh·g−1, which reaches 191.1 mAh·g−1 after 100 cycles, with a capacity retention rate of 90.7%. This study proves the feasibility of using cheap cobalt-free LLOs as cathode materials for LIBs and provides a novel system for exploiting low-cost and high-performance cathode materials.
RNAi-Mediated Silencing of Laccase 2 in Culex pipiens Pupae via Dehydration and Soaking Results in Multiple Defects in Cuticular Development
Authors: Naumenko Anastasia N. , Fritz Megan L.
Published: March 2024

Mosquitoes transmit a range of pathogens, causing devastating effects on human health. Population genetic control strategies have been developed and successfully used for several mosquito species. The most important step in identifying potential targets for mosquito control is the understanding of gene function. RNA interference (RNAi) is a powerful tool for gene silencing which has been widely used to study gene function in insects via knockdown of expression. The success of RNAi in insects depends on the efficient delivery of dsRNA into the cells, with microinjections being the most commonly used to study mosquito gene function. However, microinjections in the pupal stage lead to significant mortality in Aedes and Culex species, and few studies have performed microinjections in Culicinae pupae. Advanced techniques, such as CRISPR/Cas9 knockout, require establishing individual mosquito lines for each gene studied, and maintaining such lines may be limited by the insect-rearing capacity of a laboratory. Moreover, at times gene knockout during early development (embryo stage) has a deleterious effect on mosquito development, precluding the analysis of gene function in the pupal and adult stages and its potential for mosquito control. There is a need for a simple procedure that can be used for the fast and reliable examination of adult gene function via RNAi knockdown. Here, we focus on the aquatic stages of the mosquito life cycle and suggest a quick and easy assay for screening the functional role of genes in Culex pipiens mosquitoes without using microinjections. By dehydration of early stage pupae and subsequent rehydration in highly concentrated dsRNA, we achieved a moderate knockdown of laccase 2, a gene that turns on in the pupal stage and is responsible for melanization and sclerotization of the adult cuticle.
Robotic Lobectomy Learning Curve Has Better Clinical Outcomes than Videothoracoscopic Lobectomy
Authors: Paglialunga Pablo Luis , Molins Laureano , Guzmán Rudith , Guirao Angela , Bello Irene , Ureña Anna , Grando Leandro , Quiroga Nestor , Michavila Xavier , Boada Marc
Published: March 2024

Introduction: The robotic-assisted (RATS) lobectomy learning curve is usually measured compared to an established videothoracoscopic (VATS) surgery program. The objective of our study is to compare the learning curves of both techniques. Methods: We performed an intention-to-treat analysis comparing the RATS vs. VATS lobectomies. Surgical time, conversions, complications, number of lymph nodes (LNs) and lymph node stations harvested, chest drainage duration, length of stay, readmissions, and 90-day mortality were compared between both groups. The learning curve was assessed using the CUSUM method. Results: RATS cases (30) and VATS cases (35) displayed no significant differences. The RATS learning curve was completed after 23 procedures while the VATS curve required 28 interventions. Complications appeared in four RATS procedures and in eight VATS patients. No differences in the number of LNs and harvested LN stations were reported. Four patients were readmitted in the RATS group, and eight in the VATS group. No 90-day postoperative mortality was observed in either group. The RATS group reported fewer chest tube days (3 (2–5) vs. 5 (4–5.8), p = 0.005) and hospital days (4 (3–6) vs. 5 (4–6), p = 0.023). Conclusions: The RATS curve appears shorter than the VATS curve. RATS lobectomies resulted in reduced chest tube duration and length of stay during the learning time period.
Do Patient Sex and Age Affect Hemiepiphysiodesis Outcomes?
Authors: Morasiewicz Piotr , Leyko Paweł , Tomczyk Łukasz , Kazubski Krystian
Published: March 2024

(1) Background: The purpose of this study was to assess the effects of sex and age on the outcomes of hemiepiphysiodesis performed for genu valgum and varum deformity correction. (2) Methods: We analyzed patients who had undergone O-Plate hemiepiphysiodesis due to genu valgum or varum in the period of 2020–2023. The study group comprised 22 females and 20 males aged between 3 and 14 years at the time of surgery. Age-stratification yielded a subgroup of 3–10-year-olds (16 patients, 20 treated limbs) and a subgroup of 11–14-year-olds (26 patients, 28 treated limbs). We assessed the following parameters: hospital stay duration, deformity correction time, MAD correction, amount of angular correction, correction velocity, correction rate, complete deformity correction, deformity recurrence, surgery duration, and complications. (3) Results: The mean follow-up was 19 months. The mean surgery time in the subgroup of 3–10-year-olds (25.62 min) was significantly longer than that in the subgroup of 11–14-year-olds (22.81 min, p = 0.018). The mean deformity correction time in the male subgroup (11.33 months) was significantly shorter than that in the female subgroup (15.87 months, p = 0.013). A comparison of the subgroups stratified by age yielded a mean amount of angular correction of 10.5° in the younger children, which was significantly higher than that of 7.2° achieved in the older children; p = 0.027. The difference in mean correction velocity between 3–10-year-old children (4.03 mm/month) and that in 11–14-year-old children (1.39 mm/month) was statistically significant; p = 0.031. The mean rate of correction was 0.49°/month in females and 0.89°/month in males, with the latter rate significantly greater; p = 0.023. The difference in the mean rate of correction between the younger (1.08°/month) and the older subgroup (0.59°/month) was also significant; p = 0.018. A significant difference in terms of deformity recurrence rates was observed between the younger subgroup (66.67%) and older subgroup (only 10.53%); p = 0.005. (4) Conclusions: Patient sex had no significant effect on hemiepiphysiodesis outcomes; patient age has a considerable effect on hemiepiphysiodesis outcomes.
Radiographic Detection Rate of Distal Surface Caries in the Mandibular Second Molar in Populations with Different Third Molar Management Strategies: A Multicenter Study
Authors: Toedtling Verena , Marcov Elena-Cristina , Marcov Narcis , Bodnar Dana , Hayawi Mina W. A. , Forouzanfar Tim , Brand Henk S.
Published: March 2024

Background: Distal surface caries (DSC) has been associated with partially erupted impacted third molars. The purpose of this study was to compare the rates of DSC between populations that had undergone different third molar management strategies. Methods: Radiographs that had been taken during routine examinations of 1012, 251 and 250 patients in Manchester, Bucharest and Amsterdam, respectively, were evaluated. The following parameters were assessed: the state of the distal surface in the second mandibular molar, loss of periodontal support, impaction type of the third molar, contact point localization, and patients’ genders, ages and their cumulative history of dental health. Results: The rate of DSC in the second mandibular molar was 63.9%, 19.9% and 26.0% in the Manchester, Bucharest and Amsterdam populations, respectively. A loss of lamina dura of ≥2 mm, increased percentages of decayed, missing or filled teeth and male gender were risk factors in all three populations. All assessed parameters apart from the site of the mandible reached statistical significance in the Manchester sample (p < 0.001). The DSC rate was cumulative with increasing age in the Manchester population, in which third molars were strategically retained. Conclusions: The UK population, treated according to strict guidelines that limit the removal of third molars, had a statistically significant higher DSC prevalence rate (p < 0.001) than the Romanian or Dutch populations. The active surgical management of mandibular third molars seems to have the potential to reduce the DSC rate in the adjacent second molar.
Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light
Authors: Zmejkoski Danica Z. , Zdravković Nemanja M. , Budimir Filimonović Milica D. , Pavlović Vladimir B. , Butulija Svetlana V. , Milivojević Dušan D. , Marković Zoran M. , Todorović Marković Biljana M.
Published: March 2024

In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR, and porosity measurements. Additionally, ChiD release was assessed. The results showed that the chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape (1.5–2.5 µm). Bacterial cellulose fibers became deformed and interconnected, with increased surface roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed, and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green light. Methicillin-resistant Staphylococcus aureus was the most sensitive strain. The new photoactive composite hydrogels show promising potential for combating biofilm-related infections.
An Inclusive Civil Society Dialogue for Successful Implementation of the EU HTA Regulation: Call to Action to Ensure Appropriate Involvement of Stakeholders and Collaborators
Authors: Desmet Thomas , Julian Elaine , Van Dyck Walter , Huys Isabelle , Simoens Steven , Giuliani Rosa , Toumi Mondher , Dierks Christian , Dierks Juliana , Cardone Antonella , Houÿez Francois , Pavlovic Mira , Berntgen Michael , Mol Peter , Schiel Anja , Goettsch Wim , Gianfrate Fabrizio , Capri Stefano , Ryan James , Ducournau Pierre , Solà-Morales Oriol , Ruof Jörg
Published: March 2024

Objectives: Stakeholder involvement has long been considered a success factor for a joint European health technology assessment (HTA) process, and its relevance is now anchored in the EU HTA Regulation’s (EU HTAR) legislative wording. Therefore, we aimed to explore the roles, challenges, and most important activities to increase the level of involvement per stakeholder group. Methods: At the 2022 Fall Convention of the European Access Academy (EAA), working groups addressed the involvement of patients, clinicians, regulators, health technology developers (HTD), and national HTA bodies and payers within the EU HTA process. Each working group revisited the pre-convention survey results, determined key role characteristics for each stakeholder, and agreed on the most important activities to fulfill the role profile. Finally, the activities suggested per group were prioritized by plenary group. Results: The prioritized actions for patients included training and capacity building, the establishment of a patient involvement committee, and the establishment of a patient unit at the EC secretariat. For clinicians, it included alignment on evidence assessment from a clinical vs. HTA point of view, capacity building, and standardization of processes. The most important actions for regulators are to develop joint regulatory-HTA guidance documents, align processes and interfaces under the regulation, and share discussions on post-licensing evidence generation. HTDs prioritized scientific advice capacity and the review of the scoping process, and further development of the scope of the assessment report fact checks. The top three actions for national HTA bodies and payers included clarification on the early HTD dialogue process, political support and commitment, and clarification on financial support. Conclusions: Addressing the activities identified as the most important for stakeholders/collaborators in the EU HTA process (e.g., in the implementation of the EU HTA Stakeholder Network and of the guidance documents developed by the EUnetHTA 21 consortium) will be key to starting an “inclusive civil society dialogue”, as suggested by the European Commission’s Pharmaceutical Strategy.
Correction: Jung, W.; Chang, D. Deep Reinforcement Learning-Based Energy Management for Liquid Hydrogen-Fueled Hybrid Electric Ship Propulsion System. J. Mar. Sci. Eng. 2023, 11, 2007
Authors: Jung Wongwan , Chang Daejun
Published: March 2024

In the original publication [...]
Ship Global Traveling Path Optimization via a Novel Non-Dominated Sorting Genetic Algorithm
Authors: Zhao Shuling , Zhao Sishuo
Published: March 2024

Due to the intensification of economic globalization and the impact of global warming, the development of methods to reduce shipping costs and reduce carbon emissions has become crucial. In this study, a multi-objective optimization algorithm was designed to plan the optimal ship route for safe cross-ocean navigation under complex sea conditions. Based on the traditional non-dominated sorting genetic algorithm, considering ship stability and complex marine environment interference, a non-dominated sorting genetic algorithm model considering energy consumption was designed with the energy consumption and navigation time of the ship as the optimization objectives. The experimental results show that although the proposed method is 101.23 nautical miles more than the large ring route, and the voyage is increased by 10.1 h, the fuel consumption is reduced by 92.24 tons, saving 6.94%. Compared with the traditional genetic algorithm, the voyage distance and time are reduced by 216.93 nautical miles and 7.5 h, and the fuel consumption is reduced by 58.82 tons, which is almost 4.54%. Through experimental verification, the proposed model can obtain punctual routes, avoid areas with bad sea conditions, reduce fuel consumption, and is of great significance for improving the safety and economy of ship routes.
An Exploratory Investigation of Organic Chemicals Detected in Baby Teeth: Differences in Children with and without Autism
Authors: Palmer Raymond F.
Published: March 2024

Autism spectrum disorder (ASD) is a behaviorally defined neurodevelopmental disorder characterized by deficits in language, communication, and social function with an estimated prevalence rate of between 1 in 30 and 44 U.S. births. Gene/environment (G × E) interactions are widely regarded as the most probable explanation for idiopathic ASD, especially because some genes are selectively targeted by various environmental xenobiotics. Because deciduous teeth are a likely biomarker of in utero exposure, the present study investigated if the quantity of chemicals found in deciduous teeth differs between children with and without ASD. Twenty-two deciduous teeth from children with ASD and 20 teeth from typically developed children were prepared and analyzed using THE Two-Dimensional Gas Chromatography Time-of-Flight Mass Spectrometer (GC × GC-TOF MS) with ChromaTOF version 23H2 software and Agilent 7890 gas chromatograph. The autism sample had significantly more chemicals in their teeth than the typical developing sample (99.4 vs. 80.7, respectively) (p < 0.0001). The majority of chemicals were identified as phthalates, plasticizers, pesticides, preservatives, or intermediary solvents used in the production of fragranced personal care or cleaning products or flavoring agents in foods. The known toxic analytes reported in this study are likely biomarkers of developmental exposure. Why there were greater concentrations of toxic chemicals in the teeth that came from children with ASD is unclear. A further understanding of the cavalcade of multiple biological system interactions (Interactome) could help with future efforts to reduce risks. Notwithstanding, the avoidance of pesticides, plastics, and scented personal care products may be warranted under the precautionary principle rule.
Agricultural Yield Responses to Climate Variabilities in West Africa: A Food Supply and Demand Analysis
Authors: Schultze Martin , Kankam Stephen , Sanfo Safiétou , Fürst Christine
Published: March 2024

Agricultural productivity is expected to decrease under changing climate conditions that correspond to the stability of West African food systems. Although numerous studies have evaluated impacts of climate variability on crop yields, many uncertainties are still associated with climate extremes as well as the rapid population growth and corresponding dietary lifestyle. Here, we present a food supply and demand analysis based on the relationship between climate change, crop production, and population growth in three sites from southwestern Burkina Faso to southwestern Ghana. Climate and agricultural time series were analyzed by using boxplots mixed with a Mann–Kendall trend test and Sen’s slope. Food balance sheets were calculated by estimating the demand using a population growth model linked to food supply with local consumption patterns. We found almost insignificant rainfall and temperature trends for both sites in the Sudano-Guinean savannah. Conversely, the climate regime of southwestern Ghana revealed a strong significant increasing temperature over time. Crop yield trends demonstrated that maize and sorghum were significantly enhanced in both study areas of the Sudano-Guinean savannah. Southwestern Ghana depicted a different crop pattern where cassava and plantain showed a strong upward yield trend. The grouped food balance sheets across the regions illustrated a surplus for the Sudano-Guinean savannah while southwestern Ghana exhibited a deficit. Despite the growing yield of various crops, food demand is outpacing regional production.
The History of -eer in English: Suffix Competition or Symbiosis?
Authors: Dukic Zachary , Palmer Chris C.
Published: March 2024

Ecological models of competition have provided great explanatory power regarding synonymy in derivational morphology. Competition models of this type have certainly shown their utility, as they have demonstrated, among other things, the relevance of frequency measures, productivity, compositionality and analyzability when comparing the development of morphological constructions. There has been less consideration of alternative models that could be used to describe the historical co-development of suffixes that produce words with sometimes similar forms or meanings but are not inevitably or solely in competition. The symbiotic model proposed in this article may help answer larger questions in linguistics, such as how best to analyze certain multilingual morphological phenomena, including the emergence of semantically similar forms within the same language. The present study demonstrates the importance of a diachronic approach in situations of near-synonymy, as an understanding of semantic similarity necessitates a review of the available historical record. In particular, our study focuses on the case of the suffix -eer (e.g., marketeer) in English, analyzing its origins, semantics, compositionality, and historical development, including its symbiotic relationship to the similar suffix -er (e.g., marketer).
Narrative Review of Legal Aspects in the Integration of Simulation-Based Education into Medical and Healthcare Curricula
Authors: Slavinska Andreta , Palkova Karina , Grigoroviča Evita , Edelmers Edgars , Pētersons Aigars
Published: March 2024

The quality of healthcare varies significantly from one country to another. This variation can be attributed to several factors, including the level of healthcare professionals’ professionalism, which is closely linked to the quality of their education. Medical and healthcare education is unique in its need for students to learn and practice various clinical skills, algorithms, and behaviours for clinical situations. However, it is challenging to ensure these educational experiences do not compromise the quality of healthcare and patient safety. A simulation-based educational (SBE) approach offers a solution to these challenges. However, despite the widespread adoption of the SBE approach in medical and healthcare education curricula; its recognition for its high value among students, educators, and healthcare professionals; and evidence showing its positive impact on reducing risks to both patients and healthcare professionals, there is still an absence of a standardized approach and guidelines for integrating simulations, which includes determining when, how, and to what ex-tent they should be implemented. Currently, there is no regulation on the need for SBE integration in medical and healthcare curricula. However, the framework of this article, based on the results of the analysis of the legal framework, which includes a set of laws, regulations, principles, and standards set by various government, administrations, and authoritative institutions, will determine the fundamental aspects of the integration of the SBE approach that justify and argue the need to (1) incorporate simulation-based education across all levels of medical and healthcare education programs and (2) adhere to certain standards when integrating the SBE approach into medical and healthcare programs. This is an area that needs to be developed with the involvement of legal, health, and education experts.
Evaluating Patient Preferences and Clinical Outcomes for Modified Laparoscopic Burch Colposuspension and Transobturator Tape Procedures in Stress Urinary Incontinence Treatment
Authors: Brasoveanu Simona , Balulescu Ligia , Grigoraș Dorin , Erdelean Dragos , Olaru Flavius , Bardan Răzvan , Balint Oana , Margan Mădălin-Marius , Alexandru Alexandru , Cristiana-Smaranda Ivan , Pirtea Laurențiu
Published: March 2024

Objective: This study aims to provide an in-depth analysis of patient preferences and clinical outcomes associated with two prominent surgical techniques for treating Stress Urinary Incontinence (SUI): the modified laparoscopic Burch colposuspension and the transobturator tape (TOT) procedure. Material and Methods: A prospective cohort study was conducted on 145 patients who recieved surgical treatment for SUI, of which 71 patients (49%) underwent the modified laparoscopic Burch procedure, while 74 patients (51%) received the TOT procedure. Data on clinical characteristics, treatment success rates, and postoperative outcomes were collected and analyzed to understand patient preferences and real-world clinical effectiveness. Results: This study revealed notable differences in patient demographics and clinical characteristics between the two groups. At the 2-year follow-up, a success rate of 100% was reported in the modified laparoscopic Burch group and 86.48% in the TOT group. A total of 99 patients (68.28%) were considered cured postoperatively, with 47 (66.20%) in the modified laparoscopic Burch group and 52 (70.27%) in the TOT group (p = 0.598). A significant difference was found in the incidence of dyspareunia, with six cases (8.10%) reported in the TOT group, compared to none reported in the modified laparoscopic Burch group (p = 0.028). The median operation time was significantly shorter in the TOT group, namely 15 min, compared to the modified laparoscopic Burch group, which had a median equal to 27 min (p < 0.001). Despite these differences, patient preference for either surgical technique was observed, along with similar success rates and varied postoperative outcomes. Conclusions: The findings provide a comprehensive overview of patient preferences and factual clinical outcomes for the two surgical techniques in SUI treatment. This study contributes to understanding the factors influencing patient choice and offers valuable insights into the real-world application of these techniques, enhancing patient-centered care in SUI management.
Unusual and Unconsidered Mechanisms of Bacterial Resilience and Resistance to Quinolones
Authors: Ruiz Joaquim
Published: March 2024

Quinolone resistance has been largely related to the presence of specific point mutations in chromosomal targets, with an accessory role of impaired uptake and enhanced pump-out. Meanwhile the relevance of transferable mechanisms of resistance able to protect the target of pump-out or inactivate quinolones has been increasingly reported since 1998. Nevertheless, bacteria have other strategies and mechanisms allowing them to survive and even proliferate in the presence of quinolones, which might be qualified as resistance or resilience mechanisms. These include decreasing levels of quinolone target production, transient amoeba protection, benthonic lifestyle, nutrient-independent slow growth, activation of stringent response, inactivation or degradation of quinolones as well as apparently unrelated or forgotten chromosomal mutations. These mechanisms have been largely overlooked, either because of the use of classical approaches to antibiotic resistance determination or due to the low increase in final minimum inhibitory concentration levels. This article is devoted to a review of a series of these mechanisms.
Machine Learning-Based Predictive Model to Assess Rheological Dynamics of Eco-Friendly Oils as Biolubricants Enriched with SiO2 Nanoparticles
Authors: Hariharan Girish , Navada Meghana Kundala , Brahmavar Jeevan , Aroor Ganesha
Published: March 2024

Efficient machinery operation relies on the performance of high-quality lubricants. Currently, mineral oils of different grades are widely employed for lubricating machine components, but their environmental impact is a concern. Biolubricants are potential alternatives to mineral oils due to environmental factors. The present study focuses on assessing the rheological characteristics of SiO2 nanoparticle (NP)-enhanced ecofriendly biolubricants for near zero and high-temperature conditions. Pure neem oil, pure castor oil and a 50:50 blend of both oils were considered as the base oils. Nanobiolubricants with enhanced dispersion stability were prepared for varied concentrations of NPs using an ultrasonification method. Viscosity analysis was conducted using an MCR-92 rheometer, employing the Herschel Bulkley model to precisely characterize the viscosity behavior of bio-oils. Due to the fluid–solid interaction between SiO2 NPs and bio-oils, a crossover trend was observed in the flow curves generated for different base oils enriched with SiO2 NPs. For neem oil, a significant increase in viscosity was noted for 0.2 wt% of NPs. Using the multilayer perceptron (MLP) algorithm, an artificial neural network (ANN) model was developed to accurately predict the viscosity variations in nanobiolubricants. The accuracy of the predicted values was affirmed through experimental investigations at the considered nanoSiO2 weight concentrations.
Primary Thyroid Lymphoma: A Retrospective-Observational Study in a Single Institutional Center
Authors: Vita Octavia , Dema Alis , Barna Robert , Cornea Remus , Brebu Dan , Vlad Mihaela , Popa Oana , Muntean Ioana , Szilagyi Diana , Iacob Mihaela , Iordache Maria , Cornianu Marioara , Lazureanu Dorela Codruta
Published: March 2024

Background and Objectives: primary thyroid lymphoma (PTL) is a rare neoplasm, displaying a variety of histological features. It is often a challenge for pathologists to diagnose this tumor. Materials and Methods: this study is a retrospective analysis of clinical and pathological characteristics of a group of eleven patients (eight women and three men, mean age 68 years, range 50–80 years) diagnosed with PTL. Results: nine patients (81.81%) presented a tumor with progressive growth in the anterior cervical region, usually painless and accompanied by local compressive signs. Histologically, we identified six cases (55%) of diffuse large B-cell lymphoma, three cases (27%) of extranodal marginal zone lymphoma, one case (9%) of follicular lymphoma, and one case (9%) of mixed follicular-diffuse lymphoma. PTL was associated with microscopic Hashimoto autoimmune thyroiditis in ten cases (90.9%). Ten patients (90.9%) presented with localized disease (stage I-IIE). A percentage of 60% of patients survived over 5 years. We observed an overall longer survival in patients under 70 years of age. Conclusions: PTL represents a diagnosis that needs to be taken into account, especially in women with a history of Hashimoto autoimmune thyroiditis, presenting a cervical tumor with progressive growth. PTL is a lymphoid neoplasia with favorable outcome, with relatively long survival if it is diagnosed at younger ages.
Exercise May Increase Oxidative Stress in the Sciatic Nerve in Streptozotocin-Induced Diabetic Rats
Authors: Nonaka Koji , Akiyama Junichi , Une Satsuki
Published: March 2024

Background and Objectives: Diabetic peripheral neuropathy (DPN) affects approximately half of patients with diabetes mellitus (DM), contributing to falls and fractures. Oxidative stress, which is linked to DM-induced hyperglycemia, has been implicated in the onset of DPN. Although exercise is recommended for patients with DM, its effect on DPN remains unclear. Therefore, this study aimed to investigate the effect of exercise on DPN and the mechanisms involved. Material and Methods: Thirty male Wistar rats were divided into control, streptozotocin (STZ)-induced diabetic (DM), and STZ-induced diabetic/exercise (DM + Ex) groups. Diabetes was induced using STZ injection. Rats in the DM + Ex groups underwent six weeks of treadmill exercise. Sciatic nerve parameters, which included motor nerve conduction velocity (MNCV), antioxidant enzymes (catalase, glutathione peroxidase [GPx], and superoxide dismutase [SOD]), oxidative stress markers (malondialdehyde [MDA] and 4-hydroxy-2-nonenal [4HNE]), and neurotrophic factors (brain-derived neurotrophic factor [BDNF] and nerve growth factor [NGF]), were examined. Results: Exercise alleviated DM-induced decreases in MNCV in rats. Although exercise did not significantly affect antioxidant enzyme activity, 4HNE levels increased significantly, indicating increased oxidative stress. Additionally, exercise did not significantly affect DM-induced increases in NGF and BDNF levels in rats. Conclusions: Exercise may prevent DPN in rats with DM, possibly through nonantioxidant mechanisms.
PD-L1 Expression and Tumour Microenvironment Patterns in Resected Non-Small-Cell Lung Cancer
Authors: Gurevičienė Giedrė , Matulionė Jurgita , Poškienė Lina , Miliauskas Skaidrius , Žemaitis Marius
Published: March 2024

Background and Objectives: Although perioperative immunotherapy is implemented as a standard of care for resected non-small cell lung cancer (NSCLC), there is unmet need for predictive biomarkers as programmed death-ligand 1 (PD-L1) is not the perfect one. The functionality of tumour-infiltrating immune cells in the tumour microenvironment (TME) and the involvement in immune system response is one of the crucial factors that lead to pro- or anti-tumourigenic role and could predict response to PD-1 and PD-L1 inhibitors. So, the investigation of PD-L1 expression in the context of TME in early stages of resected NSCLC is urgent required. Materials and Methods: PD-L1 expression by three scoring methods: tumour proportion score (TPS), immune cell score (IC), and combined proportion score (CPS) was assessed in 72 archival tumour tissue specimens from stage I–III surgically resected NSCLC patients and associations with immune cells in TME were explored. Results: PD-L1 expression ≥1% evaluated by TPS, IC, and CPS was detected in 28%, 36%, and 39% of cases and moderate, substantial, and strong agreement between TPS and IC, TPS and CPS, CPS and IC was detected (Cohen’s κ coefficient 0.556, 0.63, and 0.941, respectively). PD-L1 TPS, IC, and CPS correlated with smoking intensity defined as pack-years (r = 0.0305, p = 0.012; r = 0.305, p = 0.013, and r = 0.378, p = 0.002, respectively). Only PD-L1 TPS was associated with squamous cell carcinoma (p = 0.028). PD-L1 IC ≥1% was more often seen in tumours with high CD4+ T cells infiltration (p = 0.02), while PD-L1 CPS ≥1%—in tumours with high CD4+ and CD8+ T cells infiltration (p = 0.021 and p = 0.048, respectively). PD-L1 IC and CPS ≥10% was more often detected in tumours with greater number of tumour-infiltrating CD4+Foxp3+ T cells (p = 0.01 and p = 0.025, respectively). PD-L1 TPS ≥50% was associated with higher probability to detect greater number of tumour-infiltrating M2 macrophages (p = 0.021). No association was found between PD-L1 alone or in combination with tumour-infiltrating lymphocytes, macrophages, and disease-free or overall survival. Conclusions: This study results revealed that rates of PD-L1 expression correlated among three scoring methods (TPS, IC, and CPS). Moreover, PD-L1 expression was significantly associated with smoking intensity, squamous histology, and tumour-infiltrating immune cells.
Evaluating the Clinical Utility of Brachial Plexus Block for Reducing Opioid Exposure in Pediatric Elbow Fracture Surgery: A Retrospective Cohort Study
Authors: Bae Kunhyung , Kim Yeon Ju , Lim Hyo Won , Kang Michael Seougcheol , Kim Ha-Jung , Koh Won Uk , Ro Young-jin , Cho Jooyeon , Kim Hwa Jung , Park Soo-Sung , Kwak Yoon Hae , Kim Hyungtae
Published: March 2024

Background and Objectives: Reducing opioid exposure in common pediatric surgeries is of paramount importance. This study aimed to assess the efficacy of regional nerve blocks in reducing opioid exposure while preserving high success rates. Materials and Methods: We conducted a retrospective matched cohort study (1:1) including patients with elbow fractures < 12 years old who underwent treatment with percutaneous pinning. Patients were divided into general-anesthesia (GA) and GA-followed-by-supraclavicular-brachial-plexus-block (GA-SCB) groups. The primary outcome was the number of patients administered postoperative rescue opioids. The secondary outcomes included intraoperative and postoperative opioid administration, the time to first request for rescue analgesia, pain scores, block success rate, block performing time, and block-related complications. Results: In a total of 478 patients, 363 underwent percutaneous pinning, and 86 were cohort-matched (GA: n = 43, GA-SCB: n = 43). On the first postoperative day, 34 (79.0%) patients in the GA group were administered postoperative rescue opioids, compared with 12 (27.9%) in the GA-SCB group (p < 0.001). All the patients in the GA-SCB group were opioid-free during the intraoperative period. No SCB-associated complications were observed. Total opioid consumption was significantly lower in the GA-SCB group than in the GA group until the first postoperative day (GA vs. GA-SCB, 3.2 ± 3.0 mg vs. 0.9 ± 1.8 mg, p < 0.001). Conclusions: SCB application in pediatric patients who underwent elbow fracture surgery significantly reduced opioid exposure and had a high success rate when performed using ultrasound guidance by an expert. Furthermore, the complication risk and surgical delay were minimal.
Arthroscopic Medialization Partial Repair with Biologic Interposition Tuberoplasty for Large to Massive Irreparable Rotator Cuff Tear
Authors: Jung Jae-Wook , Seo Joong-Bae , Lee Jun-Yeul , Yoo Jae-Sung
Published: March 2024

An irreparable rotator cuff tear is a challenging condition to treat, and various treatment modalities are being introduced. Medialization in the partial repair method has the limitation of exposing the tuberosity, while tension-free biologic interposition tuberoplasty using acellular dermal matrix has the limitation of exposing the humeral head. The authors believe that by combining these two techniques, it is possible to complement each other’s limitations. Therefore, they propose a surgical method that combines medialization and biologic interposition tuberoplasty for addressing these constraints.
Thermal Cracking and Friction Performance of Two Kinds of Compacted Graphite Iron Brake Discs under Intensive Braking Conditions
Authors: Xu Zhuo , Wang Guiquan , Li Yanxiang
Published: March 2024

The limited thermal conductivity of compacted graphite iron constrains its application in brake discs. The matrix plays a crucial role in balancing the thermal conductivity and mechanical performance of compacted graphite iron. Therefore, two kinds of compacted graphite brake discs with different ferrite proportions were utilized to investigate their thermal cracking and friction performance under intensive braking conditions based on inertia friction tests. The variations in peak temperature, pressure load and friction coefficient stability were also analyzed. The brake disc with a higher ferrite proportion exhibited a lower peak temperature, attributed to increased thermal conductivity. Moreover, the elevated content of soft ferrite resulted in a greater furrow height on the worn surface, contributing to an increase in friction force and stability. As a result, both the input pressure and mechanical stress decreased. It was observed that the compacted graphite iron brake disc with a higher ferrite proportion exhibited fewer thermal cracks without compromising wear resistance. Furthermore, the results suggest that lowering the disc temperature to 210 °C–250 °C can mitigate fatigue wear and matrix oxidation, hindering the propagation of thermal cracks.
High-Quality Spherical Silver Alloy Powder for Laser Powder Bed Fusion Using Plasma Rotating Electrode Process
Authors: Li Hao , Zhang Shenghuan , Chen Qiaoyu , Du Zhaoyang , Chen Xingyu , Chen Xiaodan , Zhou Shiyi , Mei Shuwen , Ke Linda , Sun Qinglei , Yin Zuowei , Yin Jie , Li Zheng
Published: March 2024

The plasma rotating electrode process (PREP) is an ideal method for the preparation of metal powders such as nickel-based, titanium-based, and iron-based alloys due to its low material loss and good degree of sphericity. However, the preparation of silver alloy powder by PREP remains challenging. The low hardness of the mould casting silver alloy leads to the bending of the electrode rod when subjected to high-speed rotation during PREP. The mould casting silver electrode rod can only be used in low-speed rotation, which has a negative effect on particle refinement. This study employed continuous casting (CC) to improve the surface hardness of S800 Ag (30.30% higher than mould casting), which enables a high rotation speed of up to 37,000 revolutions per minute, and silver alloy powder with an average sphericity of 0.98 (5.56% higher than gas atomisation) and a sphericity ratio of 97.67% (36.28% higher than gas atomisation) has been successfully prepared. The dense S800 Ag was successfully fabricated by laser powder bed fusion (LPBF), which proved the feasibility of preparing high-quality powder by the “CC + PREP” method. The samples fabricated by LPBF have a Vickers hardness of up to 271.20 HV (3.66 times that of mould casting), leading to a notable enhancement in the strength of S800 Ag. In comparison to GA, the S800 Ag powder prepared by “CC + PREP” exhibits greater sphericity, a higher sphericity ratio and less satellite powder, which lays the foundation for dense LPBF S800 Ag fabrication.
Spatiotemporal Dynamics of Assyrtiko Grape Microbiota
Authors: Tegopoulos Konstantinos , Tsirka Theodora , Stekas Christos , Gerasimidi Eleni , Skavdis George , Kolovos Petros , Grigoriou Maria E.
Published: March 2024

Vitis vinifera, an economically significant grapevine species, is known for wine, juice, and table grape production. The berries of wine grapes host a diverse range of microorganisms influencing both grapevine health and the winemaking process. Indigenous to Greece, the emblematic variety Assyrtiko, renowned for high-quality white wines, originated from Santorini and spread to various Greek regions. Despite existing studies on the microbiota of several varieties, the carposphere microbiota of Assyrtiko grapes remains unexplored. Thus, we conducted a spatiotemporal metagenomic study to identify the epiphytic microbial community composition of Assyrtiko grapes. The study was conducted in two consecutive vintage years (2019 and 2020) across three different and distinct viticulture regions in Greece (Attica, Thessaloniki, Evros). We performed amplicon sequencing, targeting the 16S rRNA gene for bacteria and the ITS region for fungi, with subsequent comprehensive bioinformatic analysis. Our data indicate that the distribution and relative abundance of the epiphytic carposphere microbial communities of the Assyrtiko variety are shaped both by vintage and biogeography.
Yeast-Based Screening of Anti-Viral Molecules
Authors: Srivastava Vartika , Kumar Ravinder , Ahmad Aijaz
Published: March 2024

Viruses are minuscule infectious agents that reproduce exclusively within the living cells of an organism and are present in almost every ecosystem. Their continuous interaction with humans poses a significant threat to the survival and well-being of everyone. Apart from the common cold or seasonal influenza, viruses are also responsible for several important diseases such as polio, rabies, smallpox, and most recently COVID-19. Besides the loss of life and long-term health-related issues, clinical viral infections have significant economic and social impacts. Viral enzymes, especially proteases which are essential for viral multiplication, represent attractive drug targets. As a result, screening of viral protease inhibitors has gained a lot of interest in the development of anti-viral drugs. Despite the availability of anti-viral therapeutics, there is a clear need to develop novel curative agents that can be used against a given virus or group of related viruses. This review highlights the importance of yeasts as an in vivo model for screening viral enzyme inhibitors. We also discuss the advantages of yeast-based screening platforms over traditional assays. Therefore, in the present article, we discuss why yeast is emerging as a model of choice for in vivo screening of anti-viral molecules and why yeast-based screening will become more relevant in the future for screening anti-viral and other molecules of clinical importance.