bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthesis, structural, spectral and photoluminescent studies of new polymorphs of Ca(II) and Ba(II) complexes of diglycolic acid
Authors: Soumya Mol U.S. , Drisya R. , Satheesh Chandran P.R. , Sudarsanakumar M.R. , Prathapachandra Kurup M.R.
Published: December 2017

Metal carboxylates possess wide range of intriguing structural topologies due to their diverse ligational behavior of carboxylate group. Two new polymorphs of monomeric complex, [Ca(dga)(H2O)5].H2O (1) and the three-dimensional metal-organic framework, [Ba(dga)(H2O)] (2) (dgaH2 = diglycolic acid) have been grown by gel diffusion technique at room temperature. Colourless transparent crystals were obtained within one week. Single crystal X-ray diffraction study reveals that the title compounds crystallized in monoclinic P21/n in 1 and orthorhombic system with space group Pnma in 2. The monomeric units, CaO8 interact each other via extensive hydrogen bonding among the coordinated and the lattice water molecules with the carboxylate oxygen atoms to form a 2D supramolecular sheet like structure in 1. The adjacent BaO8 polyhedra are connected by diglycolate ligand forming the 3D framework with porous architecture in 2. Elemental analysis, FT-IR, UV-Visible, thermogravimetric, powder X-ray diffraction and solid state photoluminescence studies were also carried out.
Synthesis and characterization of metal (M = Al or Ga) 2-phosphino(phenolate/benzenethiolate) complexes and their electrochemical behavior in the presence of CO2
Authors: Bruggen Jessica A. , Feller Keyton D. , Schickel Henry I. , Tuleushova Nazym , Zhukush Medet , Lam Kevin , Barry Brian M. , Kemp Richard A.
Published: December 2017

A series of Group 13 complexes MLX2 (M = Al or Ga, L = SC6H4-2-P tBu2 or OC6H4-2-P tBu2, X = Me or C6F5) have been synthesized and characterized by multinuclear NMR spectroscopy and single crystal X-ray diffraction. Reactions of Me3Al or Me3Ga with an equivalent of either 2- tBu2P(C6H4)OH (1) or 2- tBu2P(C6H4)SH (5) resulted in the formation of four new (2,3,6, and 7), 4-coordinate dimethyl chelate (S,P or O,P) complexes via methane elimination. The dimethyl gallium complexes (3 and 7) underwent a further reaction with excess B(C6F5)3, and through ligand exchange (methyl/pentafluorophenyl), resulted in the disubstituted bis(pentafluorophenyl) analogs (4 and 8). Cyclic voltammetry (CV) experiments for all compounds in the presence of and the absence of (1–8) CO2 were performed. For compounds showing cathodic reduction waves under CO2 (2,3,4, and 6), bulk electrolysis experiments were performed. Electrochemical studies indicate that, for several compounds, a transient CO2 adduct is formed which undergoes a one-electron, irreversible (or partially irreversible) reduction to form an unstable radical anion.
The Cardinal Difficulty for Naturalism C.S. Lewis’ Argument Reconsidered in Light of Peter van Inwagen’s Critique
Authors: Sarot Marcel
Published: December 2017

Peter van Inwagen maintains that C.S. Lewis’ argument against naturalism in Miracles fails, since Lewis has not shown that ‘Naturalism is inconsistent with ... the thesis that some of our beliefs are based on or grounded in reasoning’. In this article, Marcel Sarot shows that C.S. Lewis could not possibly have intended to argue for the inconsistency van Inwagen seems to exact, because that would amount to ‘Bulverism’, a position Lewis opposes. Furthermore, Sarot argues that Lewis did show that naturalism makes the thesis that our beliefs are based on or grounded in reasoning less likely. This, Sarot argues, is enough to make Lewis’ argument against naturalism valid.
Pragmatic Diction:Owen Barfield, the Inklings and Pragmatism
Authors: Maddalena Prof. Giovanni
Published: December 2017

Owen Barfield (1898-1997) has been a very eclectic writer: poet, novelist, and philosopher. Though almost unknown to philosophy scholars, his thought has been very influential on the work of C.S. Lewis and J.R.R. Tolkien and it is worth being studied, understood, and used in connection with pragmatism. His philosophy amounts to a strong metaphysical realism that can parallel Peirce’s view of scholastic realism and, more generally, the pragmatist attitude toward a comprehension of reality based on continuity. Moreover, Barfield sustains a view of knowledge as ‘participation’ that is very close to Peirce’s understanding of knowledge as representation. Finally, he proposes a form of ‘synthetic’ reasoning that goes the same direction as many classical pragmatists’ attempts. Therefore, the threefold philosophical aim of this paper is (1) to introduce Owen Barfield’s main theories, (2) to show the parallel between Barfield’s and pragmatists’, and especially Peirce’s tenets, and (3) to show how pragmatism and Barfield’s theory can be reciprocally useful

Men do not invent those mysterious relationsbetween separate external objects,and between objects and feelings or ideas,which it is the function of poetry to reveal.These relations exist independently, not indeed of Thoughtbut of any individual thinker. (Poetic Diction, 79)
Scripture, Setting, and Audience in the RAF Talks of C. S. Lewis
Authors: Johnson Bruce R.
Published: December 2017

It is difficult to connect individual sermons by C. S. Lewis with specific scripture texts. The wartime talks Lewis gave to RAF officers, NCOs, and airmen provide a new lens for examining this puzzle. Through biblical allusions and paraphrases, both colloquial and literary, Lewis was able to translate Holy Writ for live military audiences. Sometimes he spoke on the prescribed scripture text from the Book of Common Prayer. On other occasions, he chose a different text. When addressing committed believers, he delved deeper into explaining particular biblical passages. More often, he sent up an intellectual barrage, answering preliminary questions regarding faith, reason, and imagination. Lewis undertook an exhausting travel schedule visiting RAF stations, units, and camps throughout Britain. His creative approach to the use of scripture was one of the ways he sought to encourage military chaplains and to engage RAF aircrews and ground crews with the Christian message.
Meaning in Life and the Metaphysics of Value
Authors: Evers Daan
Published: December 2017

According to subjectivist views about a meaningful life, ones life is meaningful in virtue of desire satisfaction or feelings of fulfilment. Standard counterexamples consist of satisfaction found through trivial or immoral tasks. In response to such examples, many philosophers require that the tasks one is devoted to are objectively valuable, or have objectively valuable consequences. I argue that the counterexamples to subjectivism do not require objective value for meaning in life. I also consider other reasons for thinking that meaning in life requires objective value and raise doubts about their strength. Finally, I argue that beauty is not plausibly objective, but that it seems important for meaning. This puts pressure on the objectivist to explain why objectivity matters in the case of other values.
Conscious, Pre-Conscious and Unconscious Mechanisms in Emotional Behaviour. Some Applications to the Mindfulness Approach with Wearable Devices
Authors: Balconi Michela , Fronda Giulia , Venturella Irene , Crivelli Davide
Published: December 2017

Conscious, pre-conscious, and unconscious mechanisms are implicated in modulating affective processing in daily activities. Specifically, mental practice fostering awareness and control of affective reactions to external stimuli and stressful events (such as mindfulness and neurofeedback protocols) can be used to improve our ability to manage unconscious negative emotions. Indeed, it is possible to empower self-monitoring and regulation skills, as well as our ability to manage stress and negative emotions coming from everyday events and activities. This can be accomplished, on the one hand, by regularly practicing self-observation and by promoting bodily awareness and an awareness of automatic responses (e.g., uncontrolled affective reactions); on the other hand, by undergoing implicit training protocols that take advantage of brain responses. The present paper elucidates the contribution of both conscious and unconscious levels in emotion regulation and stress management, with a focus on their neural correlates and their role in mindfulness practice and on the potential of body-sensing devices for supporting meditation sessions, for fostering motivation to practice, and for making meditation more appealing and sustainable. We will finally present preliminary evidence on the effect of an intensive technology-mediated meditation protocol based on mindfulness practices and supported by a brain-sensing wearable device. The experimental procedure included three levels of outcome indices: psychometric measures related to perceived stress; neuropsychological and behavioural measures related to cognitive performance; and instrumental measures (resting-state and task-related electroencephalographic markers—EEG-ERPs).
Experimental Investigation on the Influence of a Double-Walled Confined Width on the Velocity Field of a Submerged Waterjet
Authors: Ding Xiaolong , Kang Yong , Li Deng , Yuan Bo , Wang Xiaochuan
Published: December 2017

The current research on confined submerged waterjets mainly focuses on the flow field of the impinging jet and wall jet. The double-sided wall vertically confined waterjet, which is widely used in many fields such as mining, cleaning and surface strengthening, has rarely been studied so far. In order to explore the influence of a double-sided wall confined width on the velocity field of submerged waterjet, an experiment was conducted with the application of 2D particle image velocimetry (PIV) technology. The distribution of mean velocity and turbulent velocity in both horizontal and vertical planes was used to characterize the flow field under various confined widths. The results show that the vertical confinement has an obvious effect on the decay rate of the mean centerline velocity. When the confined width changes from 15 to 5, the velocity is reduced by 20%. In addition, with the decrease of the confined width, the jet has a tendency to spread horizontally. The vertically confined region induces a space hysteresis effect which changes the location of the transition region moving downstream. There are local negative pressure zones separating the fluid and the wall. This study of a double-walled confined jet provides some valuable information with respect to its mechanism and industrial application.
In-Situ Heat Treatment Study on the Nanocrystalline Cr2O3 Film Using an Environmental Scanning Electron Microscope
Authors: Wang Tie-Gang , Dong Yu , Liu Yanmei , Iyengar Srinivasan , Kim Kwang , Yang Zubing
Published: December 2017

In this work, the surface morphology changes of nanocrystalline Cr2O3 film deposited on Si wafer during the heating process were observed in-situ by means of an environmental scanning electron microscope (ESEM). The Cr2O3 film cracked at high temperature due to the cause of thermal stress; the corresponding crack area percentages on the film surface were real-time evaluated using image analysis software (SISC IAS V8.0) based on the principle of gray value analysis. In the meantime, the effects of the heating temperature on the crack area percentage, phase constituents, and grain size of the Cr2O3 film were also studied in detail. The results showed that the percentage of crack area on film surface first increased with the heating temperature rise, and reached the maximum value at around 980 °C, and then gradually declined again. The above trend is closely related to the changes of thermal stress and grain growth in film. In addition, the heat treatment also had a strong influence on the grain size of the Cr2O3 film.
Progression of Renal Insufficiency in Patients with Essential Hypertension Treated with Renin Angiotensin Aldosterone System Blockers: An Electrocardiographic Correlation
Authors: Rodriguez-Padial Luis , Akerström Finn , Barderas María , Vivanco Fernando , Arias Miguel , Segura Julian , Ruilope Luis
Published: December 2017

Background: There is a frequent association between renal insufficiency and cardiovascular disease in patients with essential hypertension (HTN). The aim of this study was to analyze the relationship between ECG parameters and the progress of renal damage in patients with treated HTN. Methods: 109 patients with HTN had their microalbuminuria monitored over a 3-year time frame. During the last 3 months of follow-up, an ECG was recorded. Patients were divided into 3 groups according to the deterioration of their renal function: normoalbuminuria during the study period (normo–normo; n = 51); normoalbuminuria developing microalbuminuria (normo–micro; n = 29); and microalbuminuria at baseline (micro–micro; n = 29). Results: There were no differences in presence of left ventricular hypertrophy between the 3 groups. RV6/RV5 >1 was observed more frequently as renal function declined (p = 0.025). The 12-lead QRS-complex voltage-duration product was significantly increased in patients without microalbuminuria at baseline who went on to develop microalbuminuria (p = 0.006). Patients who developed microalbuminuria during follow-up, with positive Cornell voltage criteria, showed a lesser degree of progression of microalbuminuria when compared with the rest of the subgroups (p = 0.044). Furthermore, patients with microalbuminuria at baseline treated with angiotensin receptor blockers and diuretics, and positive Cornell voltage criteria, showed a higher degree of microalbuminuria compared to those with negative Cornell voltage criteria (p = 0.016). Conclusions: In patients with HTN, we identified some ECG parameters, which predict renal disease progression in patients with HTN, which may permit the identification of patients who are at risk of renal disease progression, despite optimal antihypertensive pharmacotherapy.
Characterisation of the Effects of Sleep Deprivation on the Electroencephalogram Using Permutation Lempel–Ziv Complexity, a Non-Linear Analysis Tool
Authors: Tosun Pinar , Abásolo Daniel , Stenson Gillian , Winsky-Sommerer Raphaelle
Published: December 2017

Specific patterns of brain activity during sleep and waking are recorded in the electroencephalogram (EEG). Time-frequency analysis methods have been widely used to analyse the EEG and identified characteristic oscillations for each vigilance state (VS), i.e., wakefulness, rapid-eye movement (REM) and non-rapid-eye movement (NREM) sleep. However, other aspects such as change of patterns associated with brain dynamics may not be captured unless a non-linear-based analysis method is used. In this pilot study, Permutation Lempel–Ziv complexity (PLZC), a novel symbolic dynamics analysis method, was used to characterise the changes in the EEG in sleep and wakefulness during baseline and recovery from sleep deprivation (SD). The results obtained with PLZC were contrasted with a related non-linear method, Lempel–Ziv complexity (LZC). Both measure the emergence of new patterns. However, LZC is dependent on the absolute amplitude of the EEG, while PLZC is only dependent on the relative amplitude due to symbolisation procedure and thus, more resistant to noise. We showed that PLZC discriminates activated brain states associated with wakefulness and REM sleep, which both displayed higher complexity, compared to NREM sleep. Additionally, significantly lower PLZC values were measured in NREM sleep during the recovery period following SD compared to baseline, suggesting a reduced emergence of new activity patterns in the EEG. These findings were validated using PLZC on surrogate data. By contrast, LZC was merely reflecting changes in the spectral composition of the EEG. Overall, this study implies that PLZC is a robust non-linear complexity measure, which is not dependent on amplitude variations in the signal, and which may be useful to further assess EEG alterations induced by environmental or pharmacological manipulations.
Analysis of Heating Expenses in a Large Social Housing Stock Using Artificial Neural Networks
Authors: Zabada Shaker , Shahrour Isam
Published: December 2017

This paper presents an analysis of heating expenses in a large social housing stock in the North of France. An artificial neural network (ANN) approach is taken for the analysis of heating consumption data collected over four years in 84 social housing residences containing 13,179 dwellings that use collective heating. Analysis provides an understanding of the influence of both physical and socio-economic parameters on heating expenses and proposes a predictive model for these expenses. The model shows that the heating expenses are influenced by both the buildings’ physical parameters and social indicators. Concerning the physical parameters, the most important indicators are the area of the dwellings, followed by the building age and the DPE (energy performance diagnostic). The family size as well as tenant age and income have an important influence on heating expense. The model is then used for establishing a data-based strategy for social housing stock renovation.
Combination of Compensations and Multi-Parameter Coil for Efficiency Optimization of Inductive Power Transfer System
Authors: Hu Guozhen , Zhang Junkun , Wang Junhua , Fang Zhijian , Cai Changsong , Lin Zhongzheng
Published: December 2017

A loosely coupled inductive power transfer (IPT) system for industrial track applications has been researched in this paper. The IPT converter using primary Inductor-Capacitor-Inductor (LCL) network and secondary parallel-compensations is analyzed combined coil design for optimal operating efficiency. Accurate mathematical analytical model and expressions of self-inductance and mutual inductance are proposed to achieve coil parameters. Furthermore, the optimization process is performed combined with the proposed resonant compensations and coil parameters. The results are evaluated and discussed using finite element analysis (FEA). Finally, an experimental prototype is constructed to verify the proposed approach and the experimental results show that the optimization can be better applied to industrial track distributed IPT system.
Tissue-Specific Transcriptome Analysis Reveals Multiple Responses to Salt Stress in Populus euphratica Seedlings
Authors: Yu Le , Ma Jianchao , Niu Zhimin , Bai Xiaotao , Lei Wenli , Shao Xuemin , Chen Ningning , Zhou Fangfang , Wan Dongshi
Published: December 2017

Salt stress is one of the most crucial factors impacting plant growth, development and reproduction. However, information regarding differences in tissue-specific gene expression patterns, which may improve a plant’s tolerance to salt stress, is limited. Here, we investigated the gene expression patterns in tissues of Populus euphratica Oliv. seedlings using RNA sequencing (RNA-Seq) technology. A total of 109.3 million, 125bp paired-end clean reads were generated, and 6428, 4797, 2335 and 3358 differentially expressed genes (DEGs) were identified in leaf, phloem, xylem and root tissues, respectively. While the tissue-specific DEGs under salt stress had diverse functions, “membrane transporter activity” was the most significant leaf function, whereas “oxidation–reduction process” was the most significant function in root tissue. Further analysis of the tissue-specific DEGs showed that the expression patterns or functions of gene families, such as SOS, NHX, GolS, GPX, APX, RBOHF and CBL, were diverse, suggesting that calcium signaling, reactive oxygen species (ROS) and salt overly sensitive (SOS) pathways are all involved in ionic homeostasis in tissues from P. euphratica seedlings. The DEGs, for example the up-regulated antioxidant genes, contribute to ROS-scavenging induced by salt stress but result in decreased Na+ concentrations in root vasculature cells and in xylem sap, while the down-regulated rbohF leads to the reverse results. These results suggest that the divergence of DEGs expression patterns contribute to maintenance of ionic and ROS homeostasis in tissues and improve plant salinity tolerance. We comprehensively analyzed the response of P. euphratica seedlings to salt stress and provide helpful genetic resources for studying plant-abiotic stress interactions.
Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata
Authors: Nguyen Hien , Miwa Hiroki , Kaneko Takakazu , Sato Shusei , Okazaki Shin
Published: December 2017

The establishment of a root nodule symbiosis between a leguminous plant and a rhizobium requires complex molecular interactions between the two partners. Compatible interactions lead to the formation of nitrogen-fixing nodules, however, some legumes exhibit incompatibility with specific rhizobial strains and restrict nodulation by the strains. Bradyrhizobium elkanii USDA61 is incompatible with mung bean (Vigna radiata cv. KPS1) and soybean cultivars carrying the Rj4 allele. Here, we explored genetic loci in USDA61 that determine incompatibility with V. radiata KPS1. We identified five novel B. elkanii genes that contribute to this incompatibility. Four of these genes also control incompatibility with soybean cultivars carrying the Rj4 allele, suggesting that a common mechanism underlies nodulation restriction in both legumes. The fifth gene encodes a hypothetical protein that contains a tts box in its promoter region. The tts box is conserved in genes encoding the type III secretion system (T3SS), which is known for its delivery of virulence effectors by pathogenic bacteria. These findings revealed both common and unique genes that are involved in the incompatibility of B. elkanii with mung bean and soybean. Of particular interest is the novel T3SS-related gene, which causes incompatibility specifically with mung bean cv. KPS1.
Context-Dependent Role of IKKβ in Cancer
Authors: Page Angustias , Navarro Manuel , Suárez-Cabrera Cristian , Bravo Ana , Ramirez Angel
Published: December 2017

Inhibitor of nuclear factor kappa-B kinase subunit beta (IKKβ) is a kinase principally known as a positive regulator of the ubiquitous transcription factor family Nuclear Factor-kappa B (NF-κB). In addition, IKKβ also phosphorylates a number of other proteins that regulate many cellular processes, from cell cycle to metabolism and differentiation. As a consequence, IKKβ affects cell physiology in a variety of ways and may promote or hamper tumoral transformation depending on hitherto unknown circumstances. In this article, we give an overview of the NF-κB-dependent and -independent functions of IKKβ. We also summarize the current knowledge about the relationship of IKKβ with cellular transformation and cancer, obtained mainly through the study of animal models with cell type-specific modifications in IKKβ expression or activity. Finally, we describe the most relevant data about IKKβ implication in cancer obtained from the analysis of the human tumoral samples gathered in The Cancer Genome Atlas (TCGA) and the Catalogue of Somatic Mutations in Cancer (COSMIC).
Activation of PPARα by Oral Clofibrate Increases Renal Fatty Acid Oxidation in Developing Pigs
Authors: He Yonghui , Khan Imad , Bai Xiumei , Odle Jack , Xi Lin
Published: December 2017

The objective of this study was to evaluate the effects of peroxisome proliferator-activated receptor α (PPARα) activation by clofibrate on both mitochondrial and peroxisomal fatty acid oxidation in the developing kidney. Ten newborn pigs from 5 litters were randomly assigned to two groups and fed either 5 mL of a control vehicle (2% Tween 80) or a vehicle containing clofibrate (75 mg/kg body weight, treatment). The pigs received oral gavage daily for three days. In vitro fatty acid oxidation was then measured in kidneys with and without mitochondria inhibitors (antimycin A and rotenone) using [1-14C]-labeled oleic acid (C18:1) and erucic acid (C22:1) as substrates. Clofibrate significantly stimulated C18:1 and C22:1 oxidation in mitochondria (p < 0.001) but not in peroxisomes. In addition, the oxidation rate of C18:1 was greater in mitochondria than peroxisomes, while the oxidation of C22:1 was higher in peroxisomes than mitochondria (p < 0.001). Consistent with the increase in fatty acid oxidation, the mRNA abundance and enzyme activity of carnitine palmitoyltransferase I (CPT I) in mitochondria were increased. Although mRNA of mitochondrial 3-hydroxy-3-methylglutaryl-coenzyme A synthase (mHMGCS) was increased, the β-hydroxybutyrate concentration measured in kidneys did not increase in pigs treated with clofibrate. These findings indicate that PPARα activation stimulates renal fatty acid oxidation but not ketogenesis.
Regulation of Aquaporin Functional Properties Mediated by the Antioxidant Effects of Natural Compounds
Authors: Pellavio Giorgia , Rui Marta , Caliogna Laura , Martino Emanuela , Gastaldi Giulia , Collina Simona , Laforenza Umberto
Published: December 2017

Some aquaporins (AQPs) have been recently demonstrated to facilitate the diffusion of hydrogen peroxide (H2O2) from the producing cells to the extracellular fluid, and their reactive oxygen species scavenging properties have been defined. Nevertheless, the identification of different AQPs acting as peroxiporins, their functional role in eustress and distress, and the identification of antioxidant compounds able to regulate AQP gating, remain unsolved. This study aims to investigate, in HeLa cells: (1) the expression of different AQPs; (2) the evaluation of naringenin, quercetin, (R)-aloesaponol III 8-methyl ether, marrubiin, and curcumin antioxidant profiles, via α,α-diphenyl-β-picrylhydrazyl assay; (3) the effect of the compounds on the water permeability in the presence and in the absence of oxidative stress; and (4) the effect of pre- and post-treatment with the compounds on the H2O2 content in heat-stressed cells. Results showed that HeLa cells expressed AQP1, 3, 8, and 11 proteins. The oxidative stress reduced the water transport, and both pre- and post-treatment with the natural compounds recovering the water permeability, with the exception of curcumin. Moreover, the pre- and post-treatment with all the compounds reduced the H2O2 content of heat-stressed cells. This study confirms that oxidative stress reduced water AQP-mediated permeability, reversed by some chemical antioxidant compounds. Moreover, curcumin was shown to regulate AQP gating. This suggests a novel mechanism to regulate cell signaling and survival during stress, and to manipulate key signaling pathways in cancer and degenerative diseases.
Learning to Teach Reinforcement Learning Agents
Authors: Fachantidis Anestis , Taylor Matthew , Vlahavas Ioannis
Published: December 2017

In this article, we study the transfer learning model of action advice under a budget. We focus on reinforcement learning teachers providing action advice to heterogeneous students playing the game of Pac-Man under a limited advice budget. First, we examine several critical factors affecting advice quality in this setting, such as the average performance of the teacher, its variance and the importance of reward discounting in advising. The experiments show that the best performers are not always the best teachers and reveal the non-trivial importance of the coefficient of variation (CV) as a statistic for choosing policies that generate advice. The CV statistic relates variance to the corresponding mean. Second, the article studies policy learning for distributing advice under a budget. Whereas most methods in the relevant literature rely on heuristics for advice distribution, we formulate the problem as a learning one and propose a novel reinforcement learning algorithm capable of learning when to advise or not. The proposed algorithm is able to advise even when it does not have knowledge of the student’s intended action and needs significantly less training time compared to previous learning approaches. Finally, in this article, we argue that learning to advise under a budget is an instance of a more generic learning problem: Constrained Exploitation Reinforcement Learning.
Correction: Škugor Rončević, I.; et al. Effective and Environmentally Friendly Nickel Coating on the Magnesium Alloy. Metals 2016, 6, 316
Authors: Rončević Ivana , Buzuk Marijo , Vladislavić Nives
Published: December 2017

The following changes have been made to the published manuscript “Effective and Environmentally Friendly Nickel Coating on the Magnesium Alloy [...]
Selenazolinium Salts as “Small Molecule Catalysts” with High Potency against ESKAPE Bacterial Pathogens
Authors: Witek Karolina , Nasim Muhammad , Bischoff Markus , Gaupp Rosmarie , Arsenyan Pavel , Vasiljeva Jelena , Marć Małgorzata , Olejarz Agnieszka , Latacz Gniewomir , Kieć-Kononowicz Katarzyna , Handzlik Jadwiga , Jacob Claus
Published: December 2017

In view of the pressing need to identify new antibacterial agents able to combat multidrug-resistant bacteria, we investigated a series of fused selenazolinium derivatives (1–8) regarding their in vitro antimicrobial activities against 25 ESKAPE-pathogen strains. Ebselen was used as reference compound. Most of the selenocompounds demonstrated an excellent in vitro activity against all S. aureus strains, with activities comparable to or even exceeding the one of ebselen. In contrast to ebselen, some selenazolinium derivatives (1, 3, and 7) even displayed significant actions against all Gram-negative pathogens tested. The 3-bromo-2-(1-hydroxy-1-methylethyl)[1,2]selenazolo[2,3-a]pyridinium chloride (1) was particularly active (minimum inhibitory concentrations, MICs: 0.31–1.24 µg/mL for MRSA, and 0.31–2.48 µg/mL for Gram-negative bacteria) and devoid of any significant mutagenicity in the Ames assay. Our preliminary mechanistic studies in cell culture indicated that their mode of action is likely to be associated with an alteration of intracellular levels of glutathione and cysteine thiols of different proteins in the bacterial cells, hence supporting the idea that such compounds interact with the intracellular thiolstat. This alteration of pivotal cysteine residues is most likely the result of a direct or catalytic oxidative modification of such residues by the highly reactive selenium species (RSeS) employed.
An Efficient PCR-RFLP Method for the Rapid Identification of Korean Pyropia Species
Authors: Kim Yonguk , Choi Sung-Je , Choi Chulyung
Published: December 2017

The present study utilizes polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using partial plastid rbcL and mitochondrial trnC–trnP gene sequences to distinguish the six representative Pyropia species produced via mariculture in Korea. The rbcL, trnC, and trnP sequences of 15 Pyropia species from the NCBI database were aligned to determine specific restriction enzyme sites of the six Pyropia species. To confirm the presence of restriction sites of eight enzymes, PCR amplicons were digested as follows: a 556 bp fragment within the rbcL region of chloroplast DNA was confirmed in P. yezoensis using BglI, whereas Tth111I, AvaII, BsrI, and BsaAI enzymes produced fragments of 664, 271, 600, and 510 bp, respectively, from the rps11–trnG region of mitochondrial DNA in P. seriata, P. dentata, P. suborbiculata, and P. haitanensis. In the case of P. pseudolinearis, HindIII, SacII, and SphI enzymes each had two cleavage sites, at positions 174 and 825, 788 and 211, and 397 and 602 bp, respectively. All six species were successfully distinguished using these eight restriction enzymes. Therefore, we propose that PCR-RFLP analysis is an efficient tool for the potential use of distinguishing between the six Pyropia species cultivated via mariculture in Korea.
Designability of Aromatic Interaction Networks at E. coli Bacterioferritin B-Type Channels
Authors: Zhang Yu , Zhou Jinhua , Ardejani Maziar S. , Li Xun , Wang Fei , Orner Brendan P.
Published: December 2017

The bacterioferritin from E. coli (BFR), a maxi-ferritin made of 24 subunits, has been utilized as a model to study the fundamentals of protein folding and self-assembly. Through structural and computational analyses, two amino acid residues at the B-site interface of BFR were chosen to investigate the role they play in the self-assembly of nano-cage formation, and the possibility of building aromatic interaction networks at B-type protein–protein interfaces. Three mutants were designed, expressed, purified, and characterized using transmission electron microscopy, size exclusion chromatography, native gel electrophoresis, and temperature-dependent circular dichroism spectroscopy. All of the mutants fold into α-helical structures and possess lowered thermostability. The double mutant D132W/N34W was 12 °C less stable than the wild type, and was also the only mutant for which cage-like nanostructures could not be detected in the dried, surface-immobilized conditions of transmission electron microscopy. Two mutants—N34W and D132W/N34W—only formed dimers in solution, while mutant D132W favored the 24-mer even more robustly than the wild type, suggesting that we were successful in designing proteins with enhanced assembly properties. This investigation into the structure of this important class of proteins could help to understand the self-assembly of proteins in general.
Peptide-Based Drugs and Drug Delivery Systems
Authors: Galdiero Stefania , Gomes Paula A. C.
Published: December 2017

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Transcriptomic Analysis Reveals Transcription Factors Related to Leaf Anthocyanin Biosynthesis in Paeonia qiui
Authors: Luo Jianrang , Duan Jingjing , Huo Dan , Shi Qianqian , Niu Lixin , Zhang Yanlong
Published: December 2017

Paeonia qiui is a wild species of tree peony. P. qiui has good ornamental value owing to its leaf color change in spring. So far, the molecular mechanism of leaf color change in P. qiui is unclear. This study analyzes the anthocyanin level and transcriptome of two different color stages in P. qiui leaf. The purplish-red leaf stage is rich in anthocyanin, while the green leaf has very low levels of anthocyanin. Transcriptome analysis reveals that 6678 differentially-expressed genes (DEGs) are up-regulated, and 14,667 are down-regulated in the purplish-red leaf. Among these DEGs, 40 MYB (v-myb avian myeloblastosis viral oncogene homolog) genes, 40 bHLH (MYC-like basic helix–loop–helix) genes, and 15 WD40 (WD-repeat protein) genes were found. Based on phylogenetic and alignment analysis with the deduced amino acid sequences with known transcription factors, Unigene0024459 (MYB1) is likely the R2R3-MYB that promotes anthocyanin biosynthesis; Unigene0050761 (MYB2) is likely the R2R3-MYB that represses anthocyanin biosynthesis; Unigene0005081 (bHLH1) and Unigene0006146 (WD40-1) are likely the bHLH and WD40 that participate in regulating anthocyanin biosynthesis. Additionally, quantitative RT-PCR results confirmed the transcriptome analyses for key genes.
Nutrition in Pregnancy Following Bariatric Surgery
Authors: Slater Christopher , Morris Lauren , Ellison Jodi , Syed Akheel
Published: December 2017

The widespread use of bariatric surgery for the treatment of morbid obesity has led to a dramatic increase in the numbers of women who become pregnant post-surgery. This can present new challenges, including a higher risk of protein and calorie malnutrition and micronutrient deficiencies in pregnancy due to increased maternal and fetal demand. We undertook a focused, narrative review of the literature and present pragmatic recommendations. It is advisable to delay pregnancy for at least 12 months following bariatric surgery. Comprehensive pre-conception and antenatal care is essential to achieving the best outcomes. Nutrition in pregnancy following bariatric surgery requires specialist monitoring and management. A multidisciplinary approach to care is desirable with close monitoring for deficiencies at each trimester.
Choosing Anthropometric Indicators to Monitor the Response to Treatment for Severe Acute Malnutrition in Rural Southern Ethiopia—Empirical Evidence
Authors: Tadesse Amare , Tadesse Elazar , Berhane Yemane , Ekström Eva-Charlotte
Published: December 2017

The World Health Organization (WHO) recommends the assessment of nutritional recovery using the same anthropometric indicator that was used to diagnose severe acute malnutrition (SAM) in children. However, related empirical evidence from low-income countries is lacking. Non-oedematous children (n = 661) aged 6–59 months admitted to a community-based outpatient therapeutic program for SAM in rural southern Ethiopia were studied. The response to treatment in children admitted to the program based on the mid-upper arm circumference (MUAC) measurement was defined by calculating the gains in average MUAC and weight during the first four weeks of treatment. The children showed significant anthropometric changes only when assessed with the same anthropometric indicator used to define SAM at admission. Children with the lowest MUAC at admission showed a significant gain in MUAC but not weight, and children with the lowest weight-for-height/length (WHZ) showed a significant gain in weight but not MUAC. The response to treatment was largest for children with the lowest anthropometric status at admission in either measurement. MUAC and weight gain are two independent anthropometric measures that can be used to monitor sufficient recovery in children treated for SAM. This study provides empirical evidence from a low-income country to support the recent World Health Organization recommendation.
Head and Neck Paraganglioma: Medical Assessment, Management, and Literature Update
Authors: Hayward Nathan , Cousins Vincent
Published: December 2017

Head and neck paraganglioma (HNPGL) are rare, highly vascular; typically slow growing and mostly benign neoplasms arising from paraganglia cells. HNPGL cause morbidity via mass effect on adjacent structures (particularly the cranial nerves), invasion of the skull base and, rarely, catecholamine secretion with associated systemic effects. The last decade has seen significant progress in the understanding of HNPGL genetics, with pertinent implications for diagnostic assessment and management of patients and their relatives. The implicated genes code for three of the five subunits of mitochondrial enzyme succinate dehydrogenase (SDH); recent literature reports that approximately one third of all HNPGL are associated with SDH mutations—a prevalence significantly greater than traditionally thought. There are distinct phenotypical syndromes associated with mutations in each individual SDH subunit (SDHD, SDHB, SDHC, and SDHAF2). This article focuses on the clinical features of HNPGL, the implications of HNPGL genetics, and the current evidence relating to optimal identification, investigation, and management options in HNPGL, which are supported by reference to a personal series of 60 cases. HNPGL require a systematic and thorough assessment to appropriately guide management decisions, and a suggested algorithm is presented in this article. Recent developments are particularly pertinent to surgeons of multiple disciplines, including otolaryngology, neurosurgery, vascular, and general surgery.
Chlorophyll Fluorescence Data Reveals Climate-Related Photosynthesis Seasonality in Amazonian Forests
Authors: Bertani Gabriel , Wagner Fabien , Anderson Liana , Aragão Luiz
Published: December 2017

Amazonia is the world largest tropical forest, playing a key role in the global carbon cycle. Thus, understanding climate controls of photosynthetic activity in this region is critical. The establishment of the relationship between photosynthetic activity and climate has been controversial when based on conventional remote sensing-derived indices. Here, we use nine years of solar-induced chlorophyll fluorescence (ChlF) data from the Global Ozone Monitoring Experiment (GOME-2) sensor, as a direct proxy for photosynthesis, to assess the seasonal response of photosynthetic activity to solar radiation and precipitation in Amazonia. Our results suggest that 76% of photosynthesis seasonality in Amazonia is explained by seasonal variations of solar radiation. However, 13% of these forests are limited by precipitation. The combination of both radiation and precipitation drives photosynthesis in the remaining 11% of the area. Photosynthesis tends to rise only after radiation increases in 61% of the forests. Furthermore, photosynthesis peaks in the wet season in about 58% of the Amazon forest. We found that a threshold of ≈1943 mm per year can be defined as a limit for precipitation phenological dependence. With the potential increase in the frequency and intensity of extreme droughts, forests that have the photosynthetic process currently associated with radiation seasonality may shift towards a more water-limited system.
Comparison of Spatial Interpolation and Regression Analysis Models for an Estimation of Monthly Near Surface Air Temperature in China
Authors: Wang Mengmeng , He Guojin , Zhang Zhaoming , Wang Guizhou , Zhang Zhengjia , Cao Xiaojie , Wu Zhijie , Liu Xiuguo
Published: December 2017

Near surface air temperature (NSAT) is a primary descriptor of terrestrial environmental conditions. In recent decades, many efforts have been made to develop various methods for obtaining spatially continuous NSAT from gauge or station observations. This study compared three spatial interpolation (i.e., Kriging, Spline, and Inversion Distance Weighting (IDW)) and two regression analysis (i.e., Multiple Linear Regression (MLR) and Geographically Weighted Regression (GWR)) models for predicting monthly minimum, mean, and maximum NSAT in China, a domain with a large area, complex topography, and highly variable station density. This was conducted for a period of 12 months of 2010. The accuracy of the GWR model is better than the MLR model with an improvement of about 3 °C in the Root Mean Squared Error (RMSE), which indicates that the GWR model is more suitable for predicting monthly NSAT than the MLR model over a large scale. For three spatial interpolation models, the RMSEs of the predicted monthly NSAT are greater in the warmer months, and the mean RMSEs of the predicted monthly mean NSAT for 12 months in 2010 are 1.56 °C for the Kriging model, 1.74 °C for the IDW model, and 2.39 °C for the Spline model, respectively. The GWR model is better than the Kriging model in the warmer months, while the Kriging model is superior to the GWR model in the colder months. The total precision of the GWR model is slightly higher than the Kriging model. The assessment result indicated that the higher standard deviation and the lower mean of NSAT from sample data would be associated with a better performance of predicting monthly NSAT using spatial interpolation models.
Scene Recognition for Indoor Localization Using a Multi-Sensor Fusion Approach
Authors: Liu Mengyun , Chen Ruizhi , Li Deren , Chen Yujin , Guo Guangyi , Cao Zhipeng , Pan Yuanjin
Published: December 2017

After decades of research, there is still no solution for indoor localization like the GNSS (Global Navigation Satellite System) solution for outdoor environments. The major reasons for this phenomenon are the complex spatial topology and RF transmission environment. To deal with these problems, an indoor scene constrained method for localization is proposed in this paper, which is inspired by the visual cognition ability of the human brain and the progress in the computer vision field regarding high-level image understanding. Furthermore, a multi-sensor fusion method is implemented on a commercial smartphone including cameras, WiFi and inertial sensors. Compared to former research, the camera on a smartphone is used to “see” which scene the user is in. With this information, a particle filter algorithm constrained by scene information is adopted to determine the final location. For indoor scene recognition, we take advantage of deep learning that has been proven to be highly effective in the computer vision community. For particle filter, both WiFi and magnetic field signals are used to update the weights of particles. Similar to other fingerprinting localization methods, there are two stages in the proposed system, offline training and online localization. In the offline stage, an indoor scene model is trained by Caffe (one of the most popular open source frameworks for deep learning) and a fingerprint database is constructed by user trajectories in different scenes. To reduce the volume requirement of training data for deep learning, a fine-tuned method is adopted for model training. In the online stage, a camera in a smartphone is used to recognize the initial scene. Then a particle filter algorithm is used to fuse the sensor data and determine the final location. To prove the effectiveness of the proposed method, an Android client and a web server are implemented. The Android client is used to collect data and locate a user. The web server is developed for indoor scene model training and communication with an Android client. To evaluate the performance, comparison experiments are conducted and the results demonstrate that a positioning accuracy of 1.32 m at 95% is achievable with the proposed solution. Both positioning accuracy and robustness are enhanced compared to approaches without scene constraint including commercial products such as IndoorAtlas.
Thermal Transmission through Existing Building Enclosures: Destructive Monitoring in Intermediate Layers versus Non-Destructive Monitoring with Sensors on Surfaces
Authors: Echarri Víctor , Espinosa Almudena , Rizo Carlos
Published: December 2017

Opaque enclosures of buildings play an essential role in the level of comfort experienced indoors and annual energy demand. The impact of solar radiation and thermal inertia of the materials that make up the multi-layer enclosures substantially modify thermal transmittance behaviour of the enclosures. This dynamic form of heat transfer, additionally affected by indoor HVAC systems, has a substantial effect on the parameters that define comfort. It also has an impact on energy demand within a daily cycle as well as throughout a one-year use cycle. This study describes the destructive monitoring of an existing block of flats located in Alicante. Once the enclosure was opened, sensors of temperature (PT100), air velocity, and relative humidity were located in the different layers of the enclosure, as well as in the interior and exterior surfaces. A pyranometer was also installed to measure solar radiation levels. A temperature data correction algorithm was drawn up to address irregularities produced in the enclosure. The algorithm was applied using a Raspberry Pi processor in the data collection system. The comparative results of temperature gradients versus non-destructive monitoring systems are presented, providing measures of the transmittance value, surface temperatures and indoor and outdoor air temperatures. This remote sensing system can be used in future studies to quantify and compare the energy savings of different enclosure construction solutions.
Characteristics Study of In-Situ Capacitive Sensor for Monitoring Lubrication Oil Debris
Authors: Han Zhibin , Wang Yishou , Qing Xinlin
Published: December 2017

As an essential part of engine health monitoring (EHM), online lubrication oil debris monitoring has recently received great attention for the assessment of rotating and reciprocating parts in aero-engines, due to its high integration, low cost and safe characteristics. However, it is be a challenge to find a suitable sensor operating in such a complex environment. We present an unconventional novel approach, in which a cylinder capacitive sensor is designed and integrated with the pipeline of an engine lubrication system, so that the capacitive sensor can effectively detect changes in the lubrication oil condition. In this paper, an attempt to illustrate the performance characteristics of the developed cylinder capacitive sensor is made, through an experiment system that simulates a real scenario of a lubrication oil system. The main aim of the research was to qualitatively describe the relationship between the sensor parameter and the lubrication oil debris. In addition, the effect of the temperature and flow rate of the lubrication oil on capacitance change was performed by several experiments and we figured out a compensation method. The experimental results demonstrated that the cylinder capacitive sensor can potentially be used for lubrication oil debris monitoring of the health condition of an aero-engine.