bluuebunny/crossref_metadata_2025 huggingface dataset:
Anatomical characteristics and potential gene mutation sites of a familial recurrent patellar dislocation
Authors: Zhang Qi-hao , Zhang Yan , He Rui-xuan , Guo Han-ming , Wang Xin-guang
Published: August 2022

Background

Recurrent patellar dislocation is the result of anatomical alignment and imbalance of restraint of bone and soft tissue. We investigate the anatomical characteristics of the knee joint in a family of patients with recurrent patella dislocation, and to screen the possible pathogenic genes in this family by whole exome sequencing in 4 patients and 4 healthy subjects, so as to provide theoretical basis for the pathogenesis of this disease.

Methods

The data related to patella dislocation were measured by imaging data. The peripheral blood DNA of related family members was extracted for the whole exome sequencing, and then the sequencing results were compared with the human database. By filtering out synonymous variants and high-frequency variants in population databases, and then integrating single nucleotide non-synonymous variants of family members, disease-causing genes were found.

Results

All patients in this family have different degrees of abnormal knee anatomy, which is closely related to patella dislocation. The sequencing results of patients and normal persons in this patella dislocation family were compared and analyzed, and the data were filtered through multiple biological databases. Find HOXB9 (NM_024017.4:c.404A>G:p.Glu135Gly),COL1A1(NM_000088.3:c.3766G>A:p.Ala1256Thr),GNPAT(NM_014236.3:c1556A>G:p.Asp519Gly),NANS(NM_018946.3:c.204G>C:p.Glu68Asp),SLC26A2(NM_000112.3:c.2065A>T:p.Thr689Ser) are nonsynonymous variants (MISSENSE). Through Sanger sequencing, the identified mutations in HOXB9 and SLC26A2 genes were only present in samples from patients with recurrent patellar dislocation.

Conclusions

The patients with recurrent patellar dislocation had markedly abnormal knee anatomy in this family. HOXB9 gene and SLC26A2 gene were found to be the possible pathogenic genes or related genes for patella dislocation.
Mucus interaction to improve gastrointestinal retention and pharmacokinetics of orally administered nano-drug delivery systems
Authors: Subramanian Deepak A. , Langer Robert , Traverso Giovanni
Published: August 2022

Oral delivery of therapeutics is the preferred route of administration due to ease of administration which is associated with greater patient medication adherence. One major barrier to oral delivery and intestinal absorption is rapid clearance of the drug and the drug delivery system from the gastrointestinal (GI) tract. To address this issue, researchers have investigated using GI mucus to help maximize the pharmacokinetics of the therapeutic; while mucus can act as a barrier to effective oral delivery, it can also be used as an anchoring mechanism to improve intestinal residence. Nano-drug delivery systems that use materials which can interact with the mucus layers in the GI tract can enable longer residence time, improving the efficacy of oral drug delivery. This review examines the properties and function of mucus in the GI tract, as well as diseases that alter mucus. Three broad classes of mucus-interacting systems are discussed: mucoadhesive, mucus-penetrating, and mucolytic drug delivery systems. For each class of system, the basis for mucus interaction is presented, and examples of materials that inform the development of these systems are discussed and reviewed. Finally, a list of FDA-approved mucoadhesive, mucus-penetrating, and mucolytic drug delivery systems is reviewed. In summary, this review highlights the progress made in developing mucus-interacting systems, both at a research-scale and commercial-scale level, and describes the theoretical basis for each type of system.
Identification and genetic diversity analysis of Rickettsia in Dermacentor nuttalli within inner Mongolia, China
Authors: Gui Zheng , Cai Hao , Qi Dong-Dong , Zhang Shun , Fu Shao-Yin , Yu Jing-Feng , Si Xiao-Yan , Cai Ting , Mao Rui
Published: August 2022

Background

The genus Rickettsia contains the lineages spotted fever group (SFG), typhus group (TG), and transitional group (TRG). The spotted fever group Rickettsia (SFGR) is transmitted by ticks. The tick species Dermacentor nuttalli is considered the main vector carrying SFGR in Inner Mongolia. Studying the genetic diversity and population structure of Rickettsia is essential for developing effective control strategies and predicting evolutionary trends of Rickettsia.

Methods

In 2019 we collected 408 D. nuttalli in the Inner Mongolia Autonomous Region, detected the percentage of Rickettsia-positive specimens, and characterized the haplotypes. From the Rickettsia-positive ticks, the gltA and ompA genes were extracted, amplified, and sequenced.

Results

Ten haplotypes of the gltA gene and 22 haplotypes of the ompA gene were obtained. The phylogenetic analysis showed that the haplotypes G1–G7 and G9 of the gltA gene cluster with Rickettsia raoultii, while G8 and G10 cluster with Rickettsia sibirica. Haplotypes O1–O15, O18 and O20–O22 of the ompA gene cluster with R. raoultii, while O16 and O19 cluster with R. sibirica. The average haplotype diversity was 0.3 for gltA and 0.7 for ompA. The average nucleotide diversity was greater than 0.05. Neutrality tests were nonsignificant for Tajima’s D results and Fu’s Fs results. The fixation index values (FST) showed that the degree of genetic differentiation between most sampled populations was small (FST < 0.05), whereas some populations showed a medium (FST > 0.05) or large (FST > 0.15) degree of differentiation. Analysis of molecular variance (AMOVA) revealed that the variation within populations was greater than that between populations. The mismatch analysis of Rickettsia showed double peaks.

Conclusions

We found two Rickettsia spp. (R. raoultii and R. sibirica). The high genetic disparity of Rickettsia allows for easy adaption to different environments. Genetic differentiation between populations is small, and Rickettsia populations do not show a geographically differentiated structure. The high rates of retention and infection of Rickettsia in D. nuttalli together with the animal husbandry exchange in Inner Mongolia gradually led to the harmonization of genetic characteristics of Rickettsia across various regions. Overall, the significant genetic diversity and geographical structure of Rickettsia in D. nuttalli are critical for SFGR control.

Graphical Abstract
Transitioning subcutaneous immunoglobulin 20% therapies in patients with primary and secondary immunodeficiencies: Canadian real-world study
Authors: Keith Paul K. , Cowan Juthaporn , Kanani Amin , Kim Harold , Lacuesta Gina , Lee Jason K. , Chen Jie , Park Michelle , Gladiator André
Published: August 2022

Background

Real-world data on transitioning to Immune Globulin Subcutaneous (Human) 20% solution (Ig20Gly) are limited. This study aimed to assess infusion parameters and experience of patients with primary (PID) or secondary immunodeficiencies (SID) transitioning to Ig20Gly in clinical practice in Canada.

Methods

Patients with PID or SID who received subcutaneous immunoglobulin (SCIG) for ≥ 3 months before transitioning to Ig20Gly were eligible for this multicenter (n = 6), phase 4, non-interventional, prospective, single-arm study. Ig20Gly infusion parameters, dosing, and adverse events were collected from patient medical records at Ig20Gly initiation and 3, 6, and 12 months post-initiation. Patient satisfaction and quality of life were assessed 12 months post-initiation using validated questionnaires.

Results

The study included 125 patients (PID, n = 60; SID, n = 64; PID + SID, n = 1). Median volume per infusion was 30.0 ml at initiation, and 40.0 ml at 6 and 12 months post-initiation. Most patients administered Ig20Gly weekly and used two infusion sites (primarily abdomen). At each time point, median infusion duration was ≤ 1 h. At 12 months, 61% of infusions were administered via a pump and 39% manually. Headache and infusion-site reactions were the most reported adverse events of interest. Patients expressed overall satisfaction with Ig20Gly at 12 months post-initiation, with all respondents indicating they would like to continue Ig20Gly.

Conclusions

This study provides a detailed description of Ig20Gly infusion parameters, tolerability, and quality of life in clinical practice among patients with PID or SID switching to Ig20Gly from another SCIG and confirms the feasibility of infusing Ig20Gly via pump or manual administration.

Trial registration NCT03716700, Registered 31 August 2018, https://clinicaltrials.gov/ct2/show/NCT03716700
The association between early formula and reduced risk of cow’s milk allergy during the first three year of life: a Japanese cohort study
Authors: Ikari Kota , Tezuka Junichiro , Sanefuji Masafumi , Nakayama Jiro , Nishima Daisuke , Sonoda Yuri , Ogawa Masanobu , Shimono Masayuki , Suga Reiko , Honjo Satoshi , Kusuhara Koichi , Ohga Shouichi ,   The Japan Environment and Children’s Study (JECS) Group, Kamijima Michihiro , Yamazaki Shin , Ohya Yukihiro , Kishi Reiko , Yaegashi Nobuo , Hashimoto Koichi , Mori Chisato , Ito Shuichi , Yamagata Zentaro , Inadera Hidekuni , Nakayama Takeo , Iso Hiroyasu , Shima Masayuki , Kurozawa Youichi , Suganuma Narufumi , Kusuhara Koichi , Katoh Takahiko
Published: August 2022

Background

Our recent observational study showed that regular consumption of cow’s milk (CM) formula during early infancy (3–6 months old) was associated with a reduced risk of CM allergy (CMA) at 12 months old. However, the long-term association is unclear. The present study was aimed to examine how long this inverse association persists after 12 months old.

Methods

This study used the dataset of an ongoing nationwide prospective cohort, the Japan Environment and Children’s Study, in which participants were registered between January 2011 and March 2014. We analyzed 65,568 children followed-up until 36 months old. The exposure factors were the consumption statuses of formula milk from 0–3, 3–6, and 6–12 months old. The primary outcome was the prevalence of CMA at 6, 12, 18, 24 and 36 months old. CMA was defined as an allergic reaction and sensitization to CM protein in an individual with no or limited intake of this protein at the evaluation time, combined with physician-diagnosed food allergy. Multivariable regression models were used to estimate the association between the periods of formula consumption and the prevalence of CMA.

Results

The prevalence of CMA increased with a peak of 1.51% at 18 months old and then declined to 0.79% at 36 months old. Formula milk from 3–6 months old was associated with a reduced risk of CMA throughout the first 3 years of life, although the extent of the reduction was mitigated with age (adjusted relative risk: [95% confidence interval]: 0.19 [0.10–0.34] at 12 months old, 0.23 [0.16–0.33] at 18 months old, 0.41 [0.26–0.64] at 24 months old, and 0.47 [0.26–0.80] at 36 months old). The association between early formula and CMA were observed in both children with and without eczema, but more prominent and long-lasting in the former than the latter.

Conclusions

Regular exposure to CM protein during infancy was associated with a reduced prevalence of CMA during early childhood. At present, however, this observational study does not necessarily encourage formula feeding, and randomized controlled trials are warranted to confirm the findings and their significance.
Adenomyoepithelioma of the breast with unusual confounding diagnostic feature: a case report
Authors: Al Mulla Liqa , Abdelhadi Maha , Al Muhanna Afnan , Elsharkawy Tarek , Al Nemer Areej
Published: August 2022

Background

Adenomyoepithelioma of the breast is an uncommon subtype of breast neoplasm that occurs in adults over a wide age range but most commonly in middle-aged and older adults. It usually presents as a solitary palpable mass or is detected on breast radiographic images. Histologically, it is a biphasic tumor with proliferation of both the epithelial and myoepithelial components of the glands, with variable types of tissue metaplasia.

Case presentation

A 64-year-old Saudi woman who underwent regular breast screening (mammogram) presented to our hospital following radiographic detection of a suspicious grouped microcalcification in the upper outer quadrant of her right breast on the mammogram. A wide local excision of the right breast lump was performed. Following histopathological examination of the breast lump, the final diagnosis was breast adenomyoepithelioma with mucoepidermoid/divergent differentiation, with no evidence of malignancy. About two years after the operation, a clinical follow-up conducted outside our hospital showed the development of ductal carcinoma in situ in the same breast.

Conclusion

Although the prognosis and the plan of treatment remains the same, our case highlights the complexities in making an accurate diagnosis between the various types of metaplasia within adenomyoepithelioma on one hand and the presence of mucoepidermoid differentiation in adenomyoepithelioma on the other.
GREM1/PPP2R3A expression in heterogeneous fibroblasts initiates pulmonary fibrosis
Authors: Shi Xiaoni , Wang Jing , Zhang Xinxin , Yang Shaoqi , Luo Wei , Wang Sha , Huang Jie , Chen Mengling , Cheng Yusi , Chao Jie
Published: August 2022

Background

Fibroblasts have important roles in the synthesis and remodeling of extracellular matrix (ECM) proteins during pulmonary fibrosis. However, the spatiotemporal distribution of heterogeneous fibroblasts during disease progression remains unknown.

Results

In the current study, silica was used to generate a mouse model of pathological changes in the lung, and single-cell sequencing, spatial transcriptome sequencing and an analysis of markers of cell subtypes were performed to identify fibroblast subtypes. A group of heterogeneous fibroblasts that play an important role at the early pathological stage were identified, characterized based on the expression of inflammatory and proliferation genes (termed inflammatory-proliferative fibroblasts) and found to be concentrated in the lesion area. The expression of GREM1/protein phosphatase 2 regulatory subunit B''alpha (PPP2R3A) in inflammatory-proliferative fibroblasts was found to initiate early pulmonary pathological changes by increasing the viability, proliferation and migration of cells.

Conclusions

Inflammatory-proliferative fibroblasts play a key role in the early pathological changes that occur in silicosis, and during this process, GREM1 is the driving factor that targets PPP2R3A and initiates the inflammatory response, which is followed by irreversible fibrosis induced by SiO2. The GREM1/PPP2R3A pathway may be a potential target in the early treatment of silicosis.
Individual-and community-level factors associated with anemia among children aged 6–23 months in sub-Saharan Africa: evidence from 32 sub-Saharan African countries
Authors: Seifu Beminate Lemma , Tesema Getayeneh Antehunegn
Published: August 2022

Background

Anemia among children aged 6–23 months is a major public health problem worldwide specifically in sub-Saharan Africa (SSA). Anemia during the childhood period causes significant short-and long-term health consequences. However, there is a paucity of evidence on Anemia among children aged 6–23 months in SSA. Therefore, this study examined the individual- and community-level factors associated with anemia among children aged 6–23 months in sub-Saharan Africa.

Methods

A secondary data analysis was done based on the most recent Demographic and Health Survey (DHS) of 32 sub-Saharan African countries. A total weighted sample of 51,044 children aged 6–23 months was included for analysis. We have used a multilevel proportional odds model to identify predictors of severity levels of anemia. Variables withp < 0.2 in the bivariable analysis were considered for the multivariable analysis. In the multivariable multilevel proportional odds model, the Adjusted Odds Ratio (AOR) with the 95% Confidence Interval (CI) was reported to declare the statistical significance and strength of the association.

Results

In this study, about 76.6% (95% CI: 76.2%, 76.9%) of children aged 6–23 months in sub-Saharan Africa were anemic. In the multivariable multilevel proportional odds model, being female, being aged 18–23 months, higher level of maternal education, being larger size at birth, belonging to a wealthier household, getting four ANC visits and above, advanced maternal age, and belonging to a community with high maternal education were significantly associated with lower odds of higher levels of anemia. On the other hand, being twin birth, being smaller size at birth, being of a higher order of birth, having fever in the last two weeks, and distance to a health facility were significantly associated with higher odds of higher levels of anemia.

Conclusion

The study found that more than three-fourths of children aged 6–23 months in sub-Saharan Africa were anemic. This finding proved that the severity levels of anemia among children in sub-Saharan Africa remain a serious public health concern. Therefore, to curve this problem enhancing maternal education, promoting maternal health service utilization, and improving health care access is crucial. In addition, health care providers better give special emphasis to twin births, higher-order birth, and those belonging to poor households to reduce the incidence of anemia among children aged 6–23 months in SSA.
Psychometric properties of the Urdu version of the EORTC QLQ-H&N35 (European organization for research and treatment of cancer head and neck module) quality of life tool
Authors: Zahid Nida , Martins Russell Seth , Zahid Wajeeha , Azam Iqbal , Ikram Mubasher , Hassan Aneesa , Bhamani Shireen Shehzad , Jabbar Adnan Abdul , Asad Nargis , Akhtar Shabbir , Siddiqui Moghira Iqbaluddin , Awan Mohammad Sohail , Ahmad Khabir
Published: August 2022

Background

We translated and validated the Urdu version of the European Organization for Research and Treatment of Cancer Quality of Life (QoL) Questionnaire’s Head and Neck (H&N) Cancer Module (EORTC QLQ-H&N35) and assessed its convergent and discriminant validity by examining correlations of QoL with depression, anxiety, and resilience.

Methods

We translated the EORTC QLQ-H&N35 according to EORTC instructions. Patients at a tertiary care hospital in Pakistan completed a survey consisting of Urdu versions of EORTC QLQ-C30 (core QoL tool), QLQ-H&N35, Hospital Anxiety and Depression Scale, and Wagnild and Young Resilience Scale (RS-14). Content validity, convergent validity, discriminant validity, and reliability (using Cronbach’s alpha) of the EORTC QLQ-H&N35 were assessed.

Results

Our sample comprised 250 patients with H&N cancer, most commonly oral (82%). The Urdu translations were comprehensible for all patients. The Cronbach alpha for QLQ-H&N35 multi-item domains ranged from 0.75 to 0.98 (acceptable to excellent), barring “Senses Problems”, which was less than the generally acceptable level (0.50). The patient-reported content validity index (CVI) scores for relevance and clarity of the Urdu version of the QLQ-H&N35 were 0.93 and 0.92, respectively (both excellent). Our results revealed weak bidirectional correlations of the QLQ-H&N35 with resilience, depression, and anxiety, showing good discriminant validity. A weak-to-moderate but significant negative correlation (r: − 0.185 to − 0.613;p < 0.01) was seen between the QLQ-H&N35 and the global QoL measure of the QLQ-30.

Conclusion

Our Urdu translation of the EORTC QLQ-H&N35 demonstrated validity comparable to previous studies, with good discriminant construct validity when measured against resilience, depression, and anxiety. An issue of concern is the poor internal consistency of the “Senses Problems” domain. Nevertheless, the Urdu translation produced in this study serves as a valid and reliable measure to measure QoL in H&N cancer in clinical or research settings in Pakistan.
The core of self-assessment and academic stress among EFL learners: the mediating role of coping styles
Authors: Jahara Syed Farhat , Hussain Mudassir , Kumar Tribhuwan , Goodarzi Ahmad , Assefa Yalalem
Published: August 2022

While several studies have addressed the interconnections between self-assessment and academic stress, much uncertainty still exists about the mediating role of coping styles in this interplay. Therefore, this research aimed to determine the mediating role of coping styles in the relationship between the core of self-assessment and academic stress. To this aim, a total of 384 English as a foreign language (EFL) learners in the training institutes in Woldia, Ethiopia, filled out three questionnaires, including self-evaluations, the coping styles, and the academic stress. The analysis of the data using structural equation modeling revealed that the core of self-assessment had significant negative effects on academic stress, emotion-based coping, and avoidance. Furthermore, self-assessment with the mediating role of other factors, namely problem-oriented coping, emotion-based coping, and avoidance coping showed to have a negative and significant effect on academic stress. Based on the findings, it is possible to receive personality traits as an individual factor, predict differences in individuals coping styles of academic stress, and, as a result, teach appropriate coping styles to EFL learners to reduce their academic stress.
Construction of magnetic nanochains to achieve magnetic energy coupling in scaffold
Authors: Shuai Cijun , Chen Xuan , He Chongxian , Qian Guowen , Shuai Yang , Peng Shuping , Deng Youwen , Yang Wenjing
Published: August 2022

Background

Fe
3
O
4
nanoparticles are highly desired for constructing endogenous magnetic microenvironment in scaffold to accelerate bone regeneration due to their superior magnetism. However, their random arrangement easily leads to mutual consumption of magnetic poles, thereby weakening the magnetic stimulation effect.

Methods

In this study, magnetic nanochains are synthesized by magnetic-field-guided interface co-assembly of Fe
3
O
4
nanoparticles. In detail, multiple Fe
3
O
4
nanoparticles are aligned along the direction of magnetic force lines and are connected in series to form nanochain structures under an external magnetic field. Subsequently, the nanochain structures are covered and fixed by depositing a thin layer of silica (SiO
2
), and consequently forming linear magnetic nanochains (Fe
3
O
4
@SiO
2
). The Fe
3
O
4
@SiO
2
nanochains are then incorporated into poly l-lactic acid (PLLA) scaffold prepared by selective laser sintering technology.

Results

The results show that the Fe
3
O
4
@SiO
2
nanochains with unique core–shell structure are successfully constructed. Meanwhile, the orderly assembly of nanoparticles in the Fe
3
O
4
@SiO
2
nanochains enable to form magnetic energy coupling and obtain a highly magnetic micro-field. The in vitro tests indicate that the PLLA/Fe
3
O
4
@SiO
2
scaffolds exhibit superior capacity in enhancing cell activity, improving osteogenesis-related gene expressions, and inducing cell mineralization compared with PLLA and PLLA/Fe
3
O
4
scaffolds.

Conclusion

In short, the Fe
3
O
4
@SiO
2
nanochains endow scaffolds with good magnetism and cytocompatibility, which have great potential in accelerating bone repair.
Muscle B mode ultrasound and shear-wave elastography in idiopathic inflammatory myopathies (SWIM): criterion validation against MRI and muscle biopsy findings in an incident patient cohort
Authors: Paramalingam Shereen , Needham Merrilee , Harris Sarah , O’Hanlon Susan , Mastaglia Frank , Keen Helen
Published: August 2022

Background

B mode ultrasound (US) and shear wave elastography (SWE) are easily accessible imaging tools for idiopathic inflammatory myopathies (IIM) but require further validation against standard diagnostic procedures such as MRI and muscle biopsy.

Methods

In this prospective cross-sectional study we compared US findings to MRI and muscle biopsy findings in a group of 18 patients (11 F, 7 M) with active IIM (dermatomyositis 6, necrotising autoimmune myopathy 7, inclusion body myositis 4, overlap myositis 1) who had one or both procedures on the same muscle. US domains (echogenicity, fascial thickness, muscle bulk, shear wave speed and power doppler) in the deltoid and vastus lateralis were compared to MRI domains (muscle oedema, fatty infiltration/atrophy) and muscle biopsy findings (lymphocytic inflammation, myonecrosis, atrophy and fibro-fatty infiltration). A composite index score (1–4) was also used as an arbitrary indicator of overall muscle pathology in biopsies.

Results

Increased echogenicity correlated with the presence of fatty infiltration/atrophy on MRI (p = 0.047) in the vastus lateralis, and showed a non-significant association with muscle inflammation, myonecrosis, fibrosis and fatty infiltration/atrophy (p > 0.333). Severe echogenicity also had a non-significant association with higher composite biopsy index score in the vastus lateralis (p = 0.380). SWS and US measures of fascial thickness and muscle bulk showed poor discrimination in differentiating between pathologies on MRI or muscle biopsy. Power Doppler measures of vascularity correlated poorly with the presence of oedema on MRI, or with inflammation or fatty infiltration on biopsy. Overall, US was sensitive in detecting the presence of muscle pathology shown on MRI (67–100%) but showed poorer specificity (13–100%). Increased echogenicity showed good sensitivity when detecting muscle pathology (100%) but lacked specificity in differentiating muscle pathologies (0%). Most study participants rated US as the preferred imaging modality.

Conclusions

Our findings show that US, in particular muscle echogenicity, has a high sensitivity, but low specificity, for detecting muscle pathology in IIM. Traditional visual grading scores are not IIM-specific and require further modification and validation. Future studies should continue to focus on developing a feasible scoring system, which is reliable and allows translation to clinical practice.
Biodiversity and virulence characterization of entomopathogenic fungi isolated from soils in different regions of Nigeria
Authors: Yakubu Mohammed Nuru , Ladan Mohammed Auwal , Deba Fatima Ali , Isma’il Safiya , Haruna Usman Sa’id , Aliyu Habiba Usman , Abdulhameed Ahmad , Tahir Fatima
Published: August 2022

Background

Studying the diversity of the indigenous entomopathogenic fungi (EPF) is a prerequisite to effective insect pest control. The distribution and occurrence of EPF from the soil across Nigeria were evaluated. Three subsoil samples from three locations in each of the 11 states were used to isolate the fungi by the insect bait method. Three fungal rates, 1.0 × 102, 5.0 × 101 and 7.5 × 101 spores/ml, were applied to the Galleria larvae.

Results

EPF occurred in 41.1% of the soil sampled. These fungi belong to five and an unidentified genus. High species diversity was observed in Abuja with 6 species, and a uniform diversity of 5 species in Gombe, Ibadan, Jos, Kano, Lagos, Nasarawa and Port Harcourt. The Shannon–Wiener index ranges from 2.48 to 1.84. Similarly, species evenness showed a distinct similarity across the 11 sampling states and ranges from 0.99 to 0.92. Simpson Diversity Index was found to be highest in Bauchi with 0.31 and lowest in Abuja with 0.19. The mortality of the Galleria mellonella (L.) larvae differed significantly (P < 0.01) with EPF species and time of exposure. There was also a strong (P < 0.001) relationship between larval mortality and conidial concentration within and among the species. The virulence of the isolated fungi on Galleria mellonella larvae based on LC50 differs with states (sampling site) even within the same species.

Conclusions

The present study uncovered the diversity and occurrence of EPF in soil across Nigeria. In addition, the results can be useful in selecting the suitable soils and best adapted EPF in a particular soil across the study area, or it could be the beginning of a molecular study.
Association of factor V Leiden R506Q, FXIIIVal34Leu, and MTHFR C677T polymorphisms with acute myocardial infarction
Authors: Golestani Amin , Rahimi Atefeh , Moridi Nastaran , Anani-Sarab Gholamreza , Salmani Fatemeh , Dastjerdi Kazem , Azdaki Nahid , Sajjadi Seyed Mehdi
Published: August 2022

Background

Acute myocardial infarction (AMI) is a leading cause of death and morbidity around the world. Although the association between thrombophilia and AMI is well-established, controversial data are present on the association between thrombophilic polymorphisms and AMI. The aim of this study was to investigate the association of three thrombophilic polymorphisms including factor V Leiden (FVL), MTHFRC677T (methylenetetrahydrofolate reductase), and Coagulation factor XIIIVal34Leu with AMI in East of Iran.

Result

There were no statistically significant differences between the patients and control groups in terms of the distributions of allelic and genotypic frequencies of FVL and FXIIIVal34Leu polymorphisms (P-value > 0.05). Subjects who carried CT genotype of MTHFR C677T polymorphism were at a 2.03-fold higher risk for AMI (P-value: 0.02, OR 1.76, 95% CI 1.07–2.75). Furthermore, patients with MTHFR 677CT (P-value < 0.001, β = - 0.90, 95% CI − 1.33, − 047) or 677CC (P-value < 0.001, β = - 1.04, 95% CI − 1.47, − 0.61) genotypes showed significantly Lower creatinine levels compared with patients having the MTHFR 677TT. No association was observed between the other remaining polymorphisms and AMI (P-value > 0.05).

Conclusion

Our findings showed that MTHFRC677T polymorphism could contribute to AMI susceptibility and increase creatinine levels in east Iran population. This was the first study to examine the association of these three polymorphisms with AMI in east Iran.
Distinct presentations and management of hernia of the umbilical cord: 15 years’ experience in a tertiary hospital
Authors: Jamal Yasir S. , Kurdi Mazen O. , Aljahdali Ettedal A. , AlBasri Samera F. , Jamal Abdullah Y.
Published: August 2022

Background

Hernia of the umbilical cord (HUC) is an uncommon type of abdominal wall defect characterized by a hernia of the midgut into the proximal section of the umbilical cord. This is occasionally coupled with other congenital abnormalities. This is frequently misdiagnosed and referred to as “omphalocele minor.” In certain cases, unintentional cord clamping causes iatrogenic intestinal harm. The purpose of this retrospective study is to highlight various aspects of the HUC therapy in 30 patients with typical and unusual presentations in a tertiary care facility as well as intraoperative findings and associated abnormalities.

Methods

Thirty neonates (21 males and 9 females) with usual and unusual presentations of HUC diagnosed and treated at the King Abdelaziz University Hospital, Jeddah, Saudi Arabia, over 15 years period from 2005 to 2020.

Results

Out of 30 cases included, 17 patients were reducible with simple classical HUC repair. While 13 patients had different presentations, six cases presented with irreducible content due to variable reasons, four cases presented with patent vitellointestinal duct (PVID), 2 cases presented with intestinal obstruction, and one case presented late with epithelialized HUC sac.

Conclusions

Attention to HUC should be paid by obstetric professionals in order not to miss it on antenatal ultrasound and careful umbilical cord examination at delivery to avoid clamping of visceral content if present in HUC.
Androgen-mediated Perturbation of the Hepatic Circadian System Through Epigenetic Modulation Promotes NAFLD in PCOS Mice
Authors: Roy Sambit , Abudu Aierken , Salinas Irving , Sinha Niharika , Cline-Fedewa Holly , Yaw Alexandra M , Qi Wenjie , Lydic Todd A , Takahashi Diana L , Hennebold Jon D , Hoffmann Hanne M , Wang Jianrong , Sen Aritro
Published: August 2022

In women, excess androgen causes polycystic ovary syndrome (PCOS), a common fertility disorder with comorbid metabolic dysfunctions including diabetes, obesity, and nonalcoholic fatty liver disease. Using a PCOS mouse model, this study shows that chronic high androgen levels cause hepatic steatosis while hepatocyte-specific androgen receptor (AR)-knockout rescues this phenotype. Moreover, through RNA-sequencing and metabolomic studies, we have identified key metabolic genes and pathways affected by hyperandrogenism. Our studies reveal that a large number of metabolic genes are directly regulated by androgens through AR binding to androgen response element sequences on the promoter region of these genes. Interestingly, a number of circadian genes are also differentially regulated by androgens. In vivo and in vitro studies using a circadian reporter [Period2::Luciferase (Per2::LUC)] mouse model demonstrate that androgens can directly disrupt the hepatic timing system, which is a key regulator of liver metabolism. Consequently, studies show that androgens decrease H3K27me3, a gene silencing mark on the promoter of core clock genes, by inhibiting the expression of histone methyltransferase, Ezh2, while inducing the expression of the histone demethylase, JMJD3, which is responsible for adding and removing the H3K27me3 mark, respectively. Finally, we report that under hyperandrogenic conditions, some of the same circadian/metabolic genes that are upregulated in the mouse liver are also elevated in nonhuman primate livers. In summary, these studies not only provide an overall understanding of how hyperandrogenism associated with PCOS affects liver gene expression and metabolism but also offer insight into the underlying mechanisms leading to hepatic steatosis in PCOS.
The blame game- experiences of female residents in obstetrics & gynecology regarding lapses in patient safety
Authors: Tufail Shazia , Mustafa Nilofar , Kamran Rizwana , Khan Junaid Sarfraz
Published: August 2022

Objectives: Primary objective was to explore experiences of female residents of Obstetrics and Gynecology (OB/GYN) regarding lapses in patient safety (PS) while secondary objective was to explore factors hampering or favouring improvement of PS in OB/GYN.

Methods: In this qualitative narrative study carried out in OB/GYN department of Combined Military Hospital (CMH) Lahore for six months from 1st April to 30th September 2021, six fourth-year residents were asked to write narratives of their personal experiences of medical error (witnessed or committed) in detail and reflect on those experiences, which were then transcribed. Code labels and themes were assigned manually. Interpretation of these themes was done after thematic content analysis.

Results: Six, fourth-year female residents with a mean age of 28.6±1.8 participated in the study. Two main themes with sub-themes were identified: 1) Challenges in patient safety (Personal challenges, Workplace challenges, Barriers to PS), 2) Lessons learnt from experiences (Self-improvement and; Promotion of patient safety culture). Heavy workload with long working hours, lack of communication and teamwork, lack of experience and inadequate supervision were major factors involved in PS lapses experienced by residents.

Conclusion: Incidents of Patient Safety (PS) lapses had a strong impact on the emotional and professional life of residents. Formal PS training with improvement of working conditions may help promote PS culture.

doi: https://doi.org/10.12669/pjms.38.7.5741

How to cite this:Tufail S, Mustafa N, Kamran R, Khan JS. The blame game- experiences of female residents in obstetrics & gynecology regarding lapses in patient safety. Pak J Med Sci. 2022;38(7):---------. doi: https://doi.org/10.12669/pjms.38.7.5741

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Hematological manifestations in patients newly diagnosed with pulmonary tuberculosis
Authors: Batool Dr Yasmeen batool , Na Dr Gulzaib Pervaiz , Na Dr Amna Arooj , Na Dr Sabeen Fatima
Published: August 2022

Objective: To determine the frequency of various hematological manifestations among newly diagnosed cases of pulmonary tuberculosis on complete blood counts.

Methods: This retrospective cross-sectional study was conducted on 500 newly diagnosed patients of pulmonary tuberculosis in hematology department of Nishtar Medical University from 1st November 2020 to 30th April 2021 using consecutive sampling. Detailed history and complete general physical examination findings, complete blood picture including erythrocyte sedimentation rate were retrieved from hospital electronic medical records system. Data was collected on a specially designed Proforma, entered into SPSS version 23.0 and analyzed. Data is presented as mean±SD and frequency and percentages for numerical and categorical variables respectively using tables and figures.

Results: In this study the mean age of patients was 34.36 ± 6.41 years with male to female ratio 2.52: 1. Frequency of anemia was 82.6% (n=413), leukocytosis was 46.2% (n=231) and leucopenia in 102 patients (20.4%). Thrombocytosis was detected in 131(26.2%) patients. Raised ESR was observed in 495(99%) of the patients. No association of age and gender was observed with hematological manifestations. However, thrombocytosis was significantly more common in male patients (p-value = 0. 008).

Conclusion: Hematological parameters like high Erythrocyte Sedimentation Rate (ESR) and anemia were commonly detected in newly diagnosed patients with pulmonary tuberculosis. Thrombocytosis was seen more commonly in male patients of pulmonary tuberculosis.

doi: https://doi.org/10.12669/pjms.38.7.5911

How to cite this:Batool Y, Pervaiz G, Arooj A, Fatima S. Hematological manifestations in patients newly diagnosed with pulmonary tuberculosis. Pak J Med Sci. 2022;38(7):---------. doi: https://doi.org/10.12669/pjms.38.7.5911

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
influence of physical activity on selected biochemical parameters - what do physically active people know about it?
Authors: Grzesznik Arleta , Formanowicz Dorota
Published: August 2022

Introduction and purpose. Physical activity is an integral part of a healthy lifestyle, and the far-reaching benefits of practising it are invaluable. Raising awareness in this regard seems to be a key element in the prevention of civilization diseases. This study aimed to assess the knowledge of amateur athletes about the influence of physical effort on changes in selected biochemical parameters. Material and methods. In the study that was conducted using the questionnaire method and electronically using the proprietary form, one hundred physically active people participated. The survey consisted of single-choice and multiple-choice questions as well as open-ended questions. It collected information on (1) demographic and social characteristics, (2) anthropometric measurements, (3) type and duration of physical activity, (4) frequency and time of the training, (5) existing diseases and (6) knowledge about glucose, insulin, lipid profile, alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase. The key questions concerned the influence of physical activity on the studied biochemical parameters. Results. The vast majority of respondents revealed a good knowledge of insulin and insulin resistance, carbohydrate metabolism and creatine kinase. The average results of correct answers amounted to 90%, 79% and 71%. The knowledge of the issues related to the lipid profile, and both ALT and AST was slightly worse. Here the distribution of the proper answers was respectively 62% and 60%. Conclusions. Properly conducted education on the influence of physical activity on selected biochemical parameters could be the best solution to broaden the knowledge on this subject.
influence of the diet on somatic differences and the developed power of athletes training strength sports
Authors: Mizera Krzysztof Maciej
Published: August 2022

Introduction: For strength training to be effective, proper nutrition is necessary, based on providing the right amount of energy, as well as optimally balanced macronutrients. Nutrition among bodybuilders, weightlifters and powerlifters differs because the training aims, training methodology and competition itself are different.

Objective: Strength sports athletes differ from each other in body structure, training methodology and form of competition. The aim of the study was to try to answer the question whether their diets affect somatic variables and leg power.

Materials and Methods: 60 people remained, which were divided into 4 groups, including 15 weightlifting competitors, 15 powerlifters, 15 bodybuilders and 15 men not training competitive sport

Results: The quality of the diet used had an impact on the body composition in the control group. In the group of powerlifters, there was a significantly positive correlation between the daily intake of carbohydrates and the percentage of body fat (p <0.05). In the group of bodybuilders, the energy value of food consumed during a week and a day as well as the mass of daily carbohydrates and their energy value significantly correlated with the BMI index. Moreover, the energy value of consumed food expressed in kcal positively correlated with the fat content in the body expressed in kg, as well as with the chest circumference during inhalation and with the circumference of the arm. The daily amount of carbohydrates and the daily energy value of this substrate positively correlated with the circumference of the arm and the thigh. In the athletes, numerous correlations between the diet used and the developed power were demonstrated.

Conclusion: The diet used by the respondents did not clearly affect the power developed by the respondents. Significant correlations between the quality of the diet used and somatic variables as well as the developed power were noticed only in the group of weightlifters and bodybuilders.
Rheumatoid arthritis – symptoms, diagnosis, treatment
Authors: Machaj Dominik , Płaczek Alicja , Cyboran Katarzyna , Siedlak Agnieszka , Białas Filip
Published: August 2022

Rheumatoid arthritis is a systemic autoimmune disease of connective tissue of unknown etiology. Specific for it is the involvement of symmetrically small and medium-sized joints, leading to degradation of their structure, stiffening and muscle contractures. It is also characterized by extra-articular and generalized symptoms, such as fatigue, low-grade fever and sweating. It can occur with or without elevated RF and / or ACPA antibodies, causes disability and contributes to premature death. Drugs for this disease can be categorized into disease-modifying drugs as well as glucocorticosteroids and non-steroidal anti-inflammatory drugs. The first-line drug is methotrexate. In case of ineffectiveness of the treatment, biological treatment can be started.
A Case of Brugada Phenocopy in Adrenal Insufficiency-Related Pericarditis
Authors:   MountainView Hospital, Las Vegas, NV USA, Kim Andrew , Valdez Cristian , Alarcon j Tony , Benge Elizabeth , Asllanaj Blerina
Published: August 2022

This is a report of a 27-year-old man with known history of Addison’s disease, noncompliant with medications, and hypothyroidism who presented with shortness of breath, nausea, vomiting, fever, and chest pain as well as Brugada sign seen on electrocardiogram. Echocardiogram revealed a moderate pericardial effusion and laboratory findings were suggestive of adrenal insufficiency. Patient was determined to have Type I Brugada phenocopy, which is a Brugada sign seen on EKG with a reversible cause. In this instance, the Brugada phenocopy was caused by adrenal insufficiency with associated pericarditis. Treatment with high-dose steroids led to resolution of both the pericardial effusion and Brugada sign, providing further evidence of Brugada phenocopy.
Mine tailings-based geopolymers: Mechanical and physical properties
Authors: He Xiang
Published: August 2022

The mining sector generates a substantial quantity of stone waste and tailings, which constitutes an environmental risk. The most prevalent method for disposing of this industrial waste is dumping, which contributes to soil deterioration and water contamination while acquiring precious land. It can be recycled using a number of processes, such as the promising geopolymerization technique, which transforms waste into value. This study reviews current developments in the manufacturing of mine tailings-based geopolymer composites from industrial waste as a possible sustainable building material. This paper also gives in-depth studies on the characteristics and behaviors of mine tailings composites used in geopolymer manufacturing, including physical and mechanical properties. This review also identifies knowledge gaps that must be filled in order to advance mine tailings composites for geopolymers.
Study on Sheet Metal Bending
Authors: John Panicker George
Published: August 2022

A sheet metal of required dimensions has been designed and modelled using CAD software. The simulation of the sheet metal is developed to understand the motion of the bending of the given sheet metal. This gives us an idea of how the spring back mechanism works. Sheet metal bending can be acquired from many different processes namely air bending, bottoming and coining processes. For this model we have considered v bending in air bending process. The designed model has been analyzed using ANSYS software, and different variables for the parameters were used to see if the spring back effect had any change. The analysis results found that spring back effect is caused by many factors like thickness, length, die and punch radius, cut cross sections near the bend area.
Mine tailings-based geopolymers: Durability, microstructure, thermal and leaching properties
Authors: Akeed Mahmoud H.
Published: August 2022

The mining industry produces a considerable amount of stone waste and tailings, posing an ecological danger. This industrial waste is often disposed of via landfill, which leads to soil degradation and water and air pollution while obtaining valuable land. It can be recycled via a variety of methods, including the promising geopolymerization approach, which converts waste into value. This research investigates recent advancements in the production of geopolymer composites derived from industrial waste and mine tailings as a potential sustainable construction material. This research also provides in-depth analyses of the features and behaviours of mine tailings mixtures utilized in geopolymer production, such as their durability, microstructure, thermal and leaching capabilities. This study also reveals an information gap that must be addressed to progress mine tailings composites for cementitious materials.
Value addition to underutilized wild Himalayan Pear

(
Pyrus Pashia): Development, Composition, and sensory characteristics of jam
Authors: SHARMA SACHIN
Published: August 2022

The present study was attempted for using the wild species Pyrus pashia found in Himalayan ranges for the production, quality evaluation and sensory evaluation of value-added product Jam. The proximate analysis of Pyrus pashia fruit showed moisture 61.8 %, reducing sugars 12.89 %, total soluble solids 14°Brix, titratable acidity 1.4 % as glucuronic acid, 53.78 ± 0.076 μg/ml IC50 value against DPPH, total phenols 173.11 ± 0.99 μg GAE/ml and total flavonoid 68.997 ± 0.83 μg RE/ml. The prepared jam J9 had, 65.79 ± 0.08 μg/ml IC50 value; J7had 80.29 ± 0.07 μg/ml IC50 value and TPC content of J7 was 130.29 ± 0.69 μg GAE/ml while J9 had 110.23± 0.94 μg GAE/ml. Sensory Evaluation Showed that J7 had better texture and appearance while J9 was loved most for its aroma and taste.
Generated artificial general intelligence
Authors: Ma Chao
Published: August 2022

my paper has been accepted by SCSET2022 and is published by IEEE-CPS.