bluuebunny/crossref_metadata_2025 huggingface dataset:
Lifetime occupation is not associated with radiographic osteoarthritis of the first metatarsophalangeal joint in a cohort study of UK women
Authors: Cherry L. , Gates L. , Arden N. K. , Bowen C. J.
Published: October 2020

Objective

The study aim was to determine whether lifetime occupation was associated with the presence of radiographic osteoarthritis (ROA) of the first metatarsophalangeal joint (MTPJ) in women.

Method

Data were collected from the prospective, population‐based Chingford 1000 Women study. This cohort of women, aged 45–64 years at inception, was established in 1989 from a single general practice in Chingford, UK. Data has subsequently been collected repeatedly. Data from baseline, year six and year ten was used for the purposes of this cross‐sectional study.

The primary outcome was the presence of dorsal view ROA of the first MTPJ. The main exposure was lifetime occupation, categorised according to levels of occupation previously defined via international consensus: 1. Sedentary, 2. Light, 3. Light manual, 4. Heavy manual. Logistic regression analyses were conducted to quantify the relationship between lifetime occupation type and the presence of ROA of the first MTPJ, adjusting for age, body mass index and lifetime high‐heeled footwear use as potential interactive variables for each decade.

Results

Data for 209 women were included within this study. The mean (SD) age was 57 (±5.2) years. Predominant lifetime occupation was reported as sedentary by 51.7%, as light by 0%, as light manual by 33.5% and as heavy manual by 14.8% of participants. There were no statistical associations between lifetime occupation type and the presence of ROA of the first MTPJ in either the unadjusted (OR = 0.99, CI = 0.78–1.26,P = 0.96) partially adjusted (for age and BMI; OR = 1.00, CI = 0.78–1.29, P = 0.99) or fully adjusted models (for age, BMI and lifetime high heel footwear use for each decade of working life (OR = 1.02, CI = 0.79–1.31, P = 0.91); high‐heel footwear use up to 20s (OR = 0.83, CI = 0.71–1.31, P = 0.83); high‐heel footwear use in 20–30s (OR = 1.00, CI = 0.75–1.3, P = 0.98); high‐heel footwear use in 30–40s (OR = 1.00, CI = 0.70–1.42, P = 0.99); high‐heel footwear use in 40–50s (OR = 0.90, CI = 0.58–1.40, P = 0.65); high‐heel footwear use in 50s (OR = 0.63,CI = 0.36–1.09, P = 0.10).

Conclusions

The findings suggest that lifetime occupation is not associated with the presence of ROA of the fist metatarsophalangeal joint. There does not appear to be any interactive effect between lifetime occupation, lifetime high‐heel footwear use, age or BMI and ROA of the first MTPJ. In later life a positive trend towards increased ROA in those who reported lifetime high‐heel footwear use was noted and this may be worthy of further research.
Bioinformatics analysis of prognostic value and prospective pathway signal of miR-30a in ovarian cancer
Authors: Lu Weijia , Wu Yunyu , Lu Can Xiong , Zhu Ting , Ren Zhong Lu , Yu Zhiwu
Published: October 2020

Objective

MicroRNAs (MiRNAs) is thought to play a critical role in the initiation and progress of ovarian cancer (OC). Although miRNAs has been widely recognized in ovarian cancer, the role of hsa-miR-30a-5p (miR-30a) in OC has not been fully elucidated.

Methods

Three mRNA datasets of normal ovarian tissue and OC, GSE18520,GSE14407 and GSE36668, were downloaded from Gene Expression Omnibus (GEO) to find the differentially expressed gene (DEG). Then the target genes of hsa-miR-30a-5p were predicted by miRWALK3.0 and TargetScan. Then, the gene overlap between DEG and the predicted target genes of miR-30a in OC was analyzed by Gene Ontology (GO) enrichment analysis. Protein-protein interaction (PPI) network was conducted by STRING and Cytoscape, and the effect of HUB gene on the outcome of OC was analyzed.

Results

A common pattern of up-regulation of miR-30a in OC was found. A total of 225 DEG, were identified, both OC-related and miR-30a-related. Many DEG are enriched in the interactions of intracellular matrix tissue, ion binding and biological process regulation. Among the 10 major Hub genes analyzed by PPI, five Hub genes were significantly related to the overall poor survival of OC patients, in which the low expression of ESR1,MAPK10, Tp53 and the high expression of YKT,NSF were related to poor prognosis of OC.

Conclusion

Our results indicate that miR-30a is of significance for the biological progress of OC.
A comparison of bupivacaine lavage and diclofenac suppository effects on post-operative pain of laparascopic transabdominal pre-peritoneal herniorrhaphy: a randomized clinical trial study
Authors: Negahi Alireza , Mousavi Seyed Hamzeh , Abbasnezhad Vahid , Jahanshahi Fatemeh
Published: October 2020

Objectives

Injection of a topical anaesthetic has been proved to be helpful with reducing pain after laparoscopic herniorrhaphy. We aimed to assess the effect of bupivacaine lavage on postoperative pain and compare it with diclofenac suppository. In this randomized clinical trial, 60 patients—scheduled for laparoscopic herniorrhaphy—were enrolled and randomized into three groups of 20 each, including diclofenac suppository, bupivacaine lavage, and normal saline as a placebo.The patients were investigated for postoperative pain scores, vomiting, nausea, morphine request, and duration of hospitalization.

Results

In the bupivacaine group, pain levels in recovery room, 4, 8 and 12 h after surgery, were significantly lower than diclofenac group; at time points of 16, 20 and 24 h after surgery, difference between two groups was not significant. Regarding vomiting and nausea, at time points of 1 and 3 h after surgery, results show no significant difference between the groups. Incident of infection, 1 h and 1 week after the surgery, was not significantly different among the groups. Duration of hospitalization in the bupivacaine group was much lower than the diclofenac group. Based on our results, use of the bupivacaine lavage can reduce postoperative pain in patients undergoing laparoscopic herniorrhaphy.

Trial Registration Randomized clinical trial IRCT20180522039782N2; date of registration:14/10/2018
Diagnostic accuracy of ultrasonography in the assessment of anterior knee pain
Authors: Basha Mohammad Abd Alkhalik , Eldib Diaa Bakry , Aly Sameh Abdelaziz , Azmy Taghreed M. , Mahmoud Nader E. M. , Ghandour Tarek Mohamed , Aly Tarek , Mostafa Shimaa , Elaidy Asmaa M. , Algazzar Hesham Youssef
Published: October 2020

Background

Anterior knee pain (AKP) is a problematic complaint, considered to be the most frequent cause of orthopedic consultancy for knee problems. This study aimed to highlight diagnostic accuracy of ultrasonography as a fast imaging technique in assessment of patients with AKP.

Methods and results

A prospective study was conducted on 143 patients with clinically confirmed AKP. All patients underwent ultrasonography and MRI examinations of the knee. The diagnostic accuracy of ultrasonography compared to MRI for evaluating different findings of possible causes of AKP were analyzed using receiver operating characteristic (ROC) curve and judged by area under curve (AUC). A total of 155 knees were included in the study; 26 knees showed no abnormalities, 19 knees showed positive MRI only, and 110 knees showed positive ultrasonography and MRI. Ultrasonography and MRI reported 11 different findings of possible causes of AKP or related to it. Joint effusion was the most common finding (38%) followed by trochlear cartilage defect (20.6%) and superficial infrapatellar subcutaneous edema (20%). The overall accuracy of ultrasonography was 85.3% sensitivity and 100% specificity. The ultrasonography provided the highest sensitivity (100%) in detecting bipartite patella, followed by 91.5% for joint effusion, and 87.5% for quadriceps tendinopathy. The ROC curve analysis of overall accuracy of ultrasonography showed an AUC of 0.93. The overall Kappa agreement between ultrasonography and MRI was good (k = 0.66).

Conclusion

Ultrasonography can be used to make a swift screening and assessment of painful anterior knee and as an alternative to MRI when it is unavailable or contraindicated.
Mesenchymal stem/stromal cell quality control: validation of mixed lymphocyte reaction assay using flow cytometry according to ICH Q2(R1)
Authors: Nicotra Tess , Desnos Aurélie , Halimi Justine , Antonot Hélène , Reppel Loïc , Belmas Thomas , Freton Alice , Stranieri Floriane , Mebarki Miryam , Larghero Jérôme , Cras Audrey , Faivre Lionel
Published: October 2020

Background

Mesenchymal stem/stromal cells (MSC) have immunomodulatory properties, studied in a wide range of diseases. Validated quality controls must confirm this activity in the context of clinical trials. This study presents a method’s validation, assessing MSC’s ability to inhibit lymphocyte proliferation, according to the ICH Q2 standard.

Methods

MSC were co-cultured with CellTrace™ Violet-labeled Peripheral blood mononuclear cells (PBMC) coming from a bank of ten donors, at seven different ratios for 7 days. Cell trace violet PBMC bank was validated in parallel. Flow cytometry analysis was used to obtain the division percentage of T cells. The percentage of inhibition of lymphocyte proliferation by MSC, for each ratio X, was calculated using the formula: Ratio × percentage of inhibition = (control percentage of division—ratio × percentage of division)/control percentage of division. The inhibition percentage of lymphocyte proliferation function of co-culture ratios was represented in a line graph. The corresponding area under the curve was calculated, representing MSC’s ability to inhibit lymphocyte proliferation.

Results

Two cell trace violet PBMC banks were compared for bank validation. When compared using four different MSC samples coming each from a different donor, their area under the curve did not show any statistical differences and were correlated. Moreover, the stability of one cell trace violet PBMC bank was confirmed up to 509 days of storage.

Analytical parameters were investigated for method validation. Analysis of repeatability and reproducibility respectively showed a standard deviation of 6.1% and 4.6%. The assay was robust regarding PBMC, as no statistical differences were found between inhibitory activities when testing three adjacent concentrations of PBMC. Still, attention is needed on MSC quantity as it can influence results. Linearity was evaluated: the percentage of inhibition of lymphocyte proliferation function of co-culture ratios was linear on the exploited range. Finally, the assay measurement range allowed to differentiate MSC presenting different inhibition activities.

Conclusion

This quantification method displayed low analytical variability and no inter-bank variability of PBMC. However, MSC quantification should be checked before co-culture to reduce variability. Therefore, it could be used for the qualification of MSC batches’ immunomodulatory activity.
Current status and future perspectives of HLA-edited induced pluripotent stem cells
Authors: Koga Keiko , Wang Bo , Kaneko Shin
Published: October 2020

In 2007, Human-induced pluripotent stem cells (iPSCs) were generated by transducing four genes (Oct3/4, Sox2, Klf4, c-Myc). Because iPSCs can differentiate into any types of cells in the body and have fewer ethical issues compared to embryonic stem (ES) cells, application of iPSCs for regenerative medicine has been actively examined. In fact, iPSCs have already been used for clinical applications, but at present, only autologous iPSC-derived grafts or HLA homozygous iPSC-derived grafts are being transplanted into patients following HLA matching. HLA is an important molecule that enables the immune system differentiates between self and non-self-components; thus, HLA mismatch is a major hurdle in the transplantation of iPSCs. To deliver inexpensive off-the-shelf iPSC-derived regenerative medicine products to more patients, it is necessary to generate universal iPSCs that can be transplanted regardless of the HLA haplotypes. The current strategy to generate universal iPSCs has two broad aims: deleting HLA expression and avoiding attacks from NK cells, which are caused by HLA deletion. Deletion of B2M and CIITA genes using the CRISPR/Cas9 system has been reported to suppress the expression of HLA class I and class II, respectively. Transduction of NK inhibitory ligands, such as HLA-E and CD47, has been used to avoid NK cell attacks. Most recently, the HLA-C retaining method has been used to generate semi-universal iPSCs. Twelve haplotypes of HLA-C retaining iPSCs can cover 95% of the global population. In future, studying which types of universal iPSCs are most effective for engraftment in various physiological conditions is necessary.
Biosensors Show Promise as a Measure of Student Engagement in a Large Introductory Biology Course
Authors: McNeal Karen S. , Zhong Min , Soltis Nick A. , Doukopoulos Lindsay , Johnson Elijah T. , Courtney Stephanie , Alwan Akilah , Porch Mallory
Published: October 2020

Skin biosensors were used to measure student engagement in an introductory biology classroom. One section of the class was taught with active-learning approaches, the other with traditional lecture. Results from galvanic skin response devices indicated students in the active-learning classroom were more engaged than those in the traditional lecture.
A Compendium of Rationales and Techniques for Active Learning
Authors: Gary Reiness C.
Published: October 2020

A review of Active Learning in College Science, a compendium of techniques for promoting active learning by students, reasons why faculty should adopt active-learning approaches, and suggestions for how to deal with resistance from faculty or students to these approaches.
Reviews
Authors: Haines Seth S.
Published: October 2020

Exploration and Production of Oceanic Natural Gas Hydrate, second edition, by Michael D. Max and Arthur H. Johnson, ISBN 978-3-030-00400-2, 2019, Springer, 482 p., US$179.99 (print), US$139 (eBook).
Study on the Optical Luminescence Properties of Li2TiO3: Mn4+ and Cr3+
Authors: Novita Mega , Marlina Dian , Ogasawara Kazuyoshi , Seok Kim Jeong , Soo Kim Yang
Published: October 2020

We studied the optical luminescence properties of Li2TiO3: Mn4+ based on first-principles calculations. The optical luminescence properties such as Mn-O distance, molecular orbital (MO) energy, crystal field splitting, multiplet energy levels, and Coulomb interaction are discussed in detail. The impact of lattice relaxation was examined through geometry optimizations based on first-principles band-structure calculations using Cambridge Serial Total Energy Package (CASTEP) software. Both Discrete Variational-Xα (DV-Xα) and the Discrete Variational Multi-electron (DVME) software were used to estimate the optical luminescence properties. Here we also predicted the optical luminescence properties of Li2TiO3: Cr3+. The results imply that the multiplet energy levels tend to decline in the order of Mn4+ to Cr3+. The integral Coulomb's decreasing tendency is in excellent agreement with the declining tendency of 2E, 2T1, 2T2 energies of Li2TiO3: Mn4+ and Li2TiO3: Cr3+.
Imaging quality and prostate MR: it is time to improve
Authors: Giganti Francesco , Allen Clare
Published: October 2020

The Prostate Imaging Reporting and Data System (PI-RADS) guidelines set out the minimal technical requirements for the acquisition of multiparametric MRI (mpMRI) of the prostate. However, the rapid diffusion of this technique has inevitably led to variability in scan quality among centres across the UK and the world. Suboptimal image acquisition reduces the sensitivity and specificity of this technique for the detection of clinically significant prostate cancer and results in clinicians losing confidence in the technique.

Two expert panels, one from the UK and one from the European Society of Urogenital Radiology (ESUR)/EAU Section of Urologic Imaging (ESUI), have stressed the importance to establish quality criteria for the acquisition of mpMRI of the prostate. A first attempt to address this issue has been the publication of the Prostate Imaging Quality (PI-QUAL) score, which assesses the mpMRI quality against a set of objective criteria (PI-RADS guidelines) together with criteria obtained from the image.

PI-QUAL represents the first step towards the standardisation of a scoring system to assess the quality of prostate mpMRI prior to reporting and allows clinicians to have more confidence in using the scan to determine patient care. Further refinements after robust consensus among experts at an international level need to be agreed before its widespread adoption in the clinical setting.
Substantia nigra micro-haemorrhage causing ipsilateral unilateral Parkinsonism and abnormal dopamine transporter scan uptake
Authors: Ghoneim Aliaa , Pollard Christopher , Tyagi Alok , Jampana Ravi
Published: October 2020

Parkinsonism is a commonly seen movement disorder syndrome with neurodegenerative and non-neurodegenerative causes. Presynaptic dopamine transporter (DaT) single‐photon emission computed tomography (SPECT) imaging is the most commonly used imaging technique in clinical practice to differentiate degenerative Parkinson’s disease (PD) and PD plus syndromes from other causes such as essential tremor and drug-induced parkinsonism. This can help identify the patients who would benefit from medical therapy due to underlying pre-synaptic dopaminergic deficits. We report a case of unilateral parkinsonism caused by ipsilateral substantia nigra micro-haemorrhage resulting in disruption of the nigrostriatal pathway. This is an unusual case of a 55-year-old male patient who presented with unilateral Parkinsonism a decade after significant head trauma where MRI plays a critical and complementary role in diagnosing complete interruption of the nigrostriatal pathway due to cerebral micro-haemorrhage. The case also beautifully demonstrates the anatomy of the nigrostriatal pathway where a small lesion in the substantia nigra caused complete loss of radioligand uptake in the ipsilateral corpus striatum. Physicians should be aware of the importance of structural imaging in atypical movement disorder cases and, in particular, the routine use of susceptibility-weighted sequences (SWI).
Evaluation of the eating habits of breast cancer patients
Authors: Kayan Tapan Tuba , Iyigun Zeynep Erdogan , Ilgun Serkan , Ozmen Vahit
Published: October 2020

Objective: To determine the relationship between the dietary characteristics of breast cancer patients.

Methods: Patients with breast cancer whose treatments have finished and are in remission formed the study group and healthy people formed the control group. Demographic, anthropometric characteristics, food consumption frequency form and exercise status were recorded with all groups. Data analysis was done by SPSS 22.

Results: In the study group, mean carbohydrate percentage was lower, while fat, fat percentage, monosaccharide, glucose, fructose, omega3(n3), saturated fatty acids(SFA), monounsaturated fatty acids (MUFA), vitamin A, C, E, B6, biotin and copper values were significantly higher (p<0.05). Recurrence was observed in seven patients (7.1%) during the follow-up period, hormone receptor levels (ER) and vitamin B2 intake (accuracy 93.9%) were inversely related to the recurrence of the disease (p=0.02).

Conclusions: While the percentage of carbohydrate taken was lower in study group; total fat, n3, SFA, MUFA, monosaccharide, glucose, fructose, water-soluble fiber, B6, biotin and copper values were higher. Further studies are needed for vitamin B2 deficiency in patients with recurrence.

doi: https://doi.org/10.12669/pjms.36.7.2368

How to cite this:Tapan TK, Iyigun ZE, Ilgun S, Ozmen V. Evaluation of the eating habits of breast cancer patients. Pak J Med Sci. 2020;36(7):1562-1566. doi: https://doi.org/10.12669/pjms.36.7.2368

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Association of peanut cultivars and aqueous neem extract in the feeding and development of Spodoptera frugiperda (J. E. Smith)
Authors: Boiça Junior Arlindo Leal , Rodrigues Nara Elisa Lobato , Janini Julio Cesar , Bottega Daline Benites , Sousa Vitor Quintela , Silva Anderson Gonçalves da
Published: October 2020

The current study aimed to test how aqueous neem Azadirachta indica A. Juss. extract affects the attractiveness, feeding and development of Spodoptera frugiperda (J. E. Smith) on different: peanut cultivars. Tests were carried out under laboratory conditions with the cultivars IAC 503 and IAC 147 and aqueous neem extract in concentrations (mass/volume) of 5 and 10%, obtained from seeds and dried in an oven at a temperature of 35 to 38 °C for 15 days, with caterpillars offered a food choice preference test. For this, leaf discs from each treatment were placed in Petri dishes into which three first instar caterpillars were released. To assess resistance biology, single newly-hatched caterpillars were transferred to individual Petri dishes, where the following biological parameters were measured: larval mortality after 1, 3, 5, 7 and 10 days; pupal mortality; total mortality; weight of 10-day-old caterpillars; pupal weight at 24 hours; and adult longevity. The main conclusions of the work, include: cultivar IAC 147 was less attractive and consumed less; cultivar IAC 503 plus 10% neem extract reduced leaf consumption; cultivar type did not affect caterpillar biological development, and 10% neem extract caused 100% mortality in S. frugiperda.
Investigating the Influence of Entrepreneurial Leadership on Students' Entrepreneurial Intentions: Teacherpreneurship as a Mediating Variable
Authors: Suyudi Muhammad , Suyatno Suyatno , Rahmatullah Azam Syukur , Rachmawati Yulia
Published: October 2020

The paper aims to know the influence of entrepreneurial leadership on the students' entrepreneurial intentions as well as its impact on teacherpreneurship. The design employed was quantitative with the ex post facto method using Partial Least Square Structural Equation Modelling (PLS-SEM) to test the hypothesis. The samples were taken using a stratified random sampling technique, obtaining 70 teachers and 285 students as the respondents. The data were gathered through a questionnaire, which has been tested for validity and reliability. The validity and reliability tests of each variable show the Cronbach's Alpha value, and the Composite Reliability is higher than 0.6 (the minimum standard), while the average variance extracted (AVE) value is higher than 0.50 (the minimum standard for validity). The hypothesis tested using the path coefficient is accepted if the t-statistic is above 1.96 and p-value less than 0.05.The results of the path coefficient and indirect effect are above 1.96 and below 0.05 for the t-statistic and p-value, respectively. Therefore, the three hypotheses are accepted. First, entrepreneurial leadership has a positive and direct significant influence on teacherpreneur, with a t-statistic value of 18.057 and a p-value 0.000. Second, similarly, the teacherpreneur positively and significantly influences students' entrepreneurial intentions, with a t-statistic value of 3.558 and a p-value 0.000. Third, entrepreneurial leadership has an indirect influence on entrepreneurial intentions, mediated by teacherpreneur. In other words, teacherpreneur mediated the influence of entrepreneurial leadership on entrepreneurial intentions, with a t-statistic value of 3.282 and p-value 0.0001. The findings recommend the importance of entrepreneurial leadership and teacherpreneur in establishing the students' entrepreneurial intentions.
Staying home: teledentistry in the delivery of paediatric dental care
Authors: Marshall Sophie , Kandiah Thayalan
Published: October 2020

Virtual clinics were introduced at Surrey and Sussex Healthcare NHS Trust in 2019 as an innovative way to improve access to care for patients. During the COVID-19 pandemic, these clinics have been key to the delivery of care to patients. This article discusses the applications and patient reported outcomes of the use of teledentistry before the COVID-19 pandemic, examines the applications of virtual clinics during the COVID-19 pandemic, and considers the role of teledentistry in routine care and patient management during the COVID-19 recovery phase. Applications in teaching are also considered along with limitations and considerations for setting up teledentistry services.

Is ‘healing at a distance’ a viable solution? We investigate the benefits and limitations of virtual clinics in managing young patients.
Phased return to dental services during the delay phase of the COVID-19 pandemic
Authors: Rodriguez Jose , Kalsi Harpoonam
Published: October 2020

A high number of patients in primary and secondary care had their dental treatments cancelled following the suspension of all primary care routine dental services in late March 2020 in response to the COVID-19 pandemic. This article outlines a protocol which can be used to classify patients according to their clinical needs and their COVID-19 risk to prioritise appointments appropriately during the delay phase of the COVID-19 pandemic. Patients should be classified into low or high clinical risk, and low or high COVID-19 risk. Both scores are entered into a matrix which gives a score that can be used to prioritise their needs. From the scores, it is possible to determine which treatments can be delayed, which patients need virtual consultations, and which patients need to be seen urgently. The scoring system can be used in primary and secondary care, across all dental specialties, and allows efficient allocation of resources, ensuring that patients with urgent clinical needs are prioritised accordingly.

Classifying patient risk using a priority scoring system will allow for appropriate workforce distribution and allocation of dental appointments.
True grit? Learning points from a facial soft tissue wound
Authors: Cole-Healy Zachary , Broderick Damian , M Graham Richard
Published: October 2020

We write this short piece regarding a recently referred case to our oral and maxillofacial surgery (OMFS) unit, which highlights several important learning points for OMFS junior staff. We describe a pertinent case in which the importance of history, examination and relevant investigations during the workup of soft tissue facial injuries is illustrated. Fundamentally, all facial traumatic wounds require a thorough assessment that is performed with a high index of suspicion of foreign body implantation.

This case report highlights that all facial traumatic wounds require a thorough assessment performed with a high index of suspicion for foreign body implantation.
The dentist will see you now
Authors: Simmons Gerald
Published: October 2020

Striking a balance between optimum dental care, staff safety and financial recovery.
First detection of small hive beetle Aethina tumida Murray (Coleoptera: Nitidulidae) infesting eastern honeybee, Apis cerana Fabricius (Hymenoptera: Apidae), in China
Authors: Zhao Hongxia , Yang Sa , Liu Jiali , Huang Wenzhong , Ji Conghui , Ren Qin , Xia Xiaoshan , Hou Chunsheng
Published: October 2020

We report the infestation of small hive beetle, Aethina tumida, in a honeybee, Apis cerana, in South China. This is the first record for domestic Chinese honey bee infested with small hive beetle.
High Number of Species of Social Wasps (Hymenoptera, Vespidae, Polistinae) Attests the Great Biodiversity of Western Amazon: a Survey from Rondônia, Brazil.
Authors: Gomes Bruno , Lima Caio Souza , Da Silva Marjorie , Noll Fernando Barbosa
Published: October 2020

A survey of social wasps (Vespidae, Polistinae), common insects of Neotropical fauna, which performs a great variety of important ecosystemic services, was conducted for the first time in areas of the Amazon forest in Rondônia state. The state is part of the western Amazon, a region harboring high biodiversity, but which has been suffering from constant deforestation. Three areas were sampled, and the wasps were actively collected, and an attractive liquid was sprayed onto the vegetation to bait the wasps. A total of 2961 wasps were sampled in all three areas, distributed in 72 species of 15 genera. Thirty-nine species were recorded for the first time in the state and three others (Agelaia melanopyga Cooper, Brachygastra cooperiAndena and Carpenter and Polybia diguetana du Buysson)represents the first record for Brazil.AgelaiaLepeletier was the most abundant genus in all areas, which is congruent with results of other surveys, and the greatest species richness was found for Polybia Lepeletier. The highest number of species was sampled in Floresta Nacional do Jamari (51), followed by Estação Ecológica de Cuniã (46) and forest fragment of Universidade Federal de Rondônia (39). The latter also presented the highest number of different genera sampled. The great diversity sampled, mainly for Epiponini, which represented 64 of the 72 species collected, can be attributed to various factor as the location of the areas and also to the methodology used.
Occurrence of social wasps (Hymenoptera: Vespidae) in farming of organic tobacco (Nicotiana tabacum L.) in south of Brazil
Authors: Schoeninger Karine , Somavilla Alexandre , Kohler Andreas
Published: October 2020

This work aims to recognize and record the occurrence of species of social wasps (Polistinae) in organic tobacco crops, as well as to point out possible species that may be used in future biological control programs. The research was conducted in Virginia-type tobacco farming with organic management in two regions in south of Brazil. The collections were carried out with Malaise trap, during the harvests 2008/2009, 2009/2010 and 2010/2011. A total of 2.738 wasps, from which 23 species distributed in six genera was collected. Polistes was the most diverse genus (8 species), followed by Polybia, Mischocyttarus, Agelaia, Bachygastra and Protonectarina. Polybia scutellaris was the most abundant species, being considered eudominant. Agelaia multipicta and Polybia sericea with are also among the most abundant. This information is relevant for the insertion of social wasps with potential agents in the biological control of tobacco pests.
Multiple Use of an Old Nest by the European Paper Wasp Polistes dominula (Hymenoptera, Vespidae) in Central Poland
Authors: Łączyński Piotr
Published: October 2020

Refurbishing the old nest in order to prepare it for a new breeding season cycle has already been observed in few species of paper wasps. When future foundresses emerge from hibernacula they have choose between building a new nest or reusing an old one. In present paper I described a case of multiple use and further expansion an old nest by Polistes dominula (Christ, 1791) in Grodzisk Mazowiecki, Mazovia Region, Central Poland.
Community Structure and Composition of Social Wasps (Hymenoptera: Vespidae) in Different Vegetation Types in São Paulo, Brazil
Authors: Clemente Mateus Aparecido , Guevara Roger , Moleiro Hugo Ribeiro , Silveira Orlando Tobias , Giannotti Edillberto
Published: October 2020

Social wasps participate in food webs, act as biological control agents in small crops and the natural environment, and act as potential pollinators. The objective of this study was to carry out an inventory of social wasps in five phytophysiognomies with different degrees of anthropogenic disturbance in São Paulo, Brazil. The collections were performed by active search and attractive traps to survey social wasps. We collected wasp for one year with collection campaigns every other month totaling six campaigns for site. We collected a total of 913 individuals representing 31 species and eight genera. The species with the highest abundances were Agelaia pallipes and Agelaia vicina. The highest richness of social wasps was found in the riparian forest and regenerating Cerrado. We conclude that social wasp richness and diversity varies according to habitat vegetation characteristics and that these effects are likely mediated by resulting differences in the provision of resources and nesting locations among the different environments. The areas in this study have suffered considerable vegetation loss due to common farming practices, however, the existing fragments are still capable of hosting a rich social wasp fauna.
Non-Inseminated Queens Have Worker-Like Behaviors in Colonies of Fungus-Growing Ants, Mycetomoellerius turrifex Wheeler (Attini, Hymenoptera)
Authors: Murakami Takahiro
Published: October 2020

In  eusocial  Hymenoptera  such  as  ants,  bees,  and  wasps,  the  queen  numbers  are  fundamentally  important  to  maintain  the  social  systems.  In  Texas,  USA,  a  fungus  growing  ant,  Mycetomoellerius  turrifex  Wheeler  (1903)  (the  genus  name  was  changed from Trachymyrmex to Mycetomoellerius in 2019 (Solomon et al., 2019)) was  observed  to  have  several  non-inseminated  queens  that  wandered  outside  the nest long after the mating season. However, the evolutionary and ecological factors causing the occurrence of such non-inseminated queens are still unclear. Thus,  I  examined  the  worker-like  behaviors  of  non-inseminated  queens  of  M. turrifex in Texas. Fifteen ant colonies were collected over three years, between 1999 and 2001. The frequencies of non-inseminated queens, workers, and broods, as well as the depths of nest chambers, were observed in each collecting year. In Nov. 1999 and May 2000, multiple nests contained queens that did not mate with males within their nests. These nests had a relatively larger colony size than those collected in Oct. 2001. Conversely, the colonies collected in Oct. 2001 were completely monogynous, i.e., there were no non-inseminated queens in the nests. Behavioral observations of each female revealed that the non-inseminated queensbehaved significantly differently from the workers and the inseminated queens. The behaviors that distinguished different female castes were mutualistic fungus garden care, digging of nest floors, guarding, and resting in the nest. These data suggest that queens failed to mate due to severe environmental fluctuations in southern Texas, but were accepted by the colony as a temporary labor force.
EFEK AROMATERAPI MINYAK ATSIRI MAWAR (Rosa damascena MILL.) DAN KULIT JERUK LIMAU (Citrus amblycarpa) TERHADAP JUMLAH MIKROBA UDARA RUANGAN BERPENDINGIN
Authors: Komala Oom , Utami Novi Fajar , Rosdiana Siti Mariyam
Published: October 2020

Energy, material, and or other components were entered to the air by activities of human could cause air quality down to a specific level that could affect human health. The bacteria in the human oral cavity such as Staphylococcus sp. were found in the air during common activities such as coughing, sneezing, and speaking. The research aimed to assess the influence of the combination of oils essential of Rosa damacena and leather oil of Citrus amblycarpa against a decline in the total of microbes on air-conditioned rooms. The method of the research  was used the evaporation of aromatherapy and plate count method. The results were showed that P3 with the concentration of oils essential of R. damacena 2% and leather oil of C. amblycarpa 5% most effective could decrease the total of microbes with an average of 72.5%. Organoleptic, relative density, refractive index, solubility in 90% ethanol, and acid number analysis of Rosa damacena and Citrus amblycarpa leather essential oils met the Indonesian National Standard requirements except for the acid number of leatherlime essential oil and the optical rotation of rose essential oil. It is concluded that the combination of oils essential R. damacena and leather oil of C. amblycarpa  could reduce the total of microbes on air-conditioned rooms.
HUBUNGAN PANJANG-BOBOT DAN FAKTOR KONDISI IKAN NILEM (Osteochilus vittatus VALENCIENNES, 1842) DI PERAIRAN WADUK BENANGA, KALIMANTAN TIMUR
Authors: Jusmaldi Jusmaldi , Hariani Nova , Wulandari Nikmahtulhaniah Ayu
Published: October 2020

Biology of bonylip barb (Osteochilus vittatus Valenciennes, 1842) in Benanga Reservoir, East Kalimantan is not yet known. The purpose of this study was to analyze distribution of the length and body weight of fishes, sex ratio, gonad maturity level, length-body weight relationship, and condition factor. In total 278 fishes samples were collected during the five months, from January to May 2019 with a purposive sampling method at Benanga Reservoir, and gill net with  mesh size 0.5; 1; and 1.5  inches used. The results of this research showed that the total length of the fishes analyzed ranged from 72.21–196.57 mm and body weight ranged from 5.12–89.21 g. The sex ratio of male and female were 1:2.02. The male and female fishes were dominated by mature gonad. The length-body weight relationship models calculated for the total fishes sample was W=1x10-5L2.967 (r=0.988) for males, W=5x10-6L3.199 (r=0.989) for females and W=7x10-6L3.126 (r=0.987) for both sexes. Isometric growth patterns (b=3) were observed in males, meanwhile positive allometric growth patterns (b>3) were observed in females and both sexes. Calculation of the condition factor (K) resulted in 1.181±0.117 for males, 0.949±0.094 for females, and 1.026±0.150 for all specimens. The condition factors of bonylip barb from the Benanga Reservoir inside the range recommended as suitable for freshwater fish species in the tropical regions.
ISOLASI DAN UJI KOMPATIBILITAS BAKTERI HIDROLITIK DARI TANAH TEMPAT PEMROSESAN AKHIR TALANGAGUNG, KABUPATEN MALANG
Authors: Fitriasari Prilya Dewi , Amalia Nanda , Farkhiyah Susiyanti
Published: October 2020

Talangagung landfill, Malang Regency has used the controlled landfill method. The landfill process produces methane gas that has been flowed as fuel so that it is estimated that there are bacteria with a variety of enzymatic activities that have worked in degrading waste. The purpose of this study was to isolate bacteria from landfill and determine the ability to produce amylase, protease, lipase, and cellulase and test the compatibility of bacteria. The methods used include bacterial isolation using the pour plate method and purification of bacterial isolates using the streak plate method. Bacterial selection is done on selective media containing starch, tributyrin, cellulose, and skim milk. There were 21 bacterial isolates successfully isolated 13 consisting of bacterial isolates showed proteolytic activity, 10 cellulolytic bacteria, 8 amylolytic, and 15 lipolytic. The isolates that show compatible results are BTA 5.7.14, BTA 5.7.7, BTA 5.7.12, and BTA 5.7.6 therefore they can be used as a potential consortium for organic waste recycle.
PENGARUH MEDIA TERKONDISI SEL PUNCA MESENSIMAL TERHADAP EKSPRESI GEN TRANSCRIPTION FACTOR 7-LIKE 2 (TCF7L2) TIKUS MODEL DIABETES MELITUS TIPE 2
Authors: Widhiastuti Stefani Santi , Branitamahisi Bernadia , Inayati Nor Sri , Preharsini Ida Ayu , Handika Demas Bayu , Sadewa Ahmad Hamim , Laqif Abdurahman , Haryana Sofia Mubarika
Published: October 2020

Diabetes mellitus type 2 is the most common type of diabetes. This study was conducted to determine the effect of Mesenchymal Stem Cell-Conditioned Medium (MSC-CM) in Homeostatic Model Assessment of β-cell function (HOMA-β) value, normal Langerhans cells, and Transcription Factor 7-Like 2 (TCF7L2) gene expression in type 2 diabetic rats model. As many as 27 male Sprague Dawley rats were divided into 3 study research groups: normal control (9 normal rats), diabetic control (9 type 2 diabetic rats, induced by 60 mg/kg BW Streptozotocin and 120 mg/kg BW Nicotinamide i.p.), and treatment (9 type 2 diabetic rats treatment with 0.1 ml/200g BW MSC-CM i.p.). On day 30 after therapy, the expression of TCF7L2 gene was performed with real time-quantitative PCR (RT-qPCR). The HOMA-β value were calculated based on Fasting Insulins (FINs) levels and Fasting Blood Glucose (FBG) levels data from other research team members. Based on results, MSC-CM increases the HOMA-β value and amount of normal Langerhans cells of treatment group that indicates amelioration effect of MSC-CM, but there was no significant difference in TCF7L2 gene expression level between diabetic control and treatment group.
CHROMOSOME COUNT ON YOUNG ANTHER OF BANANA MALE BUD USING EZYMATIC MACERATION AND DAPI STAINING IN SLIDE PREPARATION
Authors: Ahmad Fajarudin , Poerba Yuyu Suryasari
Published: October 2020

Chromosome counting is the basis in describing the chromosomes number of organism that might useful for genetic study and classification. In banana studies, the root tip with a combination of non-fluorescent staining methods such as carmine or orcein and squash is the most common material for chromosome counting. In this study, we presented the usefulness of young anther of banana male bud with enzymatic maceration method for cell spreading and 4,6-diamino-2-phenyl-indole (DAPI) for staining agent to get a satisfying chromosomes image at metaphase for mitotic study of diploid and tetraploid bananas. The principle of this study is fixation using ethanol:acetic acid (3:1), enzymatic digestion, maceration and staining using DAPI. Our result showed that this method can provide well spread cells with intensely contrast of chromosomes images that satisfying for chromosome counting.
JUMLAH, UJI VIABILITAS DAN DAYA KECAMBAH POLEN 31 AKSESI PISANG (Musa sp.) KOLEKSI KEBUN PLASMA NUTFAH PISANG LIPI
Authors: Hasrianda Erwin Fajar , Zaelani Ahmad , Poerba Yuyu Suryasari
Published: October 2020

Cross pollination is one of the plant breeding techniques to create a better cultivar with desired traits. A successful cross pollination is affected by quality and quantity of the pollen used. The aim from this study is to know amounts, viability and germination from various banana accessions. This study was conducted by using 31 banana accessions. Pollen quantity per anther, pollen viability, pollen germination growth were measured. The results showed that pollen amounts were ranged between 5.032 and 118.181 pollens per anther. In addition, pollen viability ranged from 30% in Tingalun accession (Musa sp. cv. Tingalun)  to 99% in Malaccensis 2x accession. Furthermore, pollen germination level varied from 1% in Mambee Thu OS accession to 48% in Rutilifes 2x accession. We also found that negative correlation between pollen viability and pollen germination were moderate with correlation coeeficient value of -0,59 ( p-value = 0,00051). From 31 banana accessions tested in this research, pollen source from Malaccensis 2x and Rutilifes 2x banana accessions have the highest potential to be used as the male parent.
VEGETASI POHON DAN PERSEBARANNYA DI TAMAN WISATA ALAM GUNUNG TUNAK DAN HUTAN KERAMAT, MANDALIKA, LOMBOK TENGAH, PROVINSI NUSA TENGGARA BARAT
Authors: Mansur Muhammad
Published: October 2020

The study was conducted on March 2018 in two locations study, i.e. Mount Tunak Nature Tourism Park (NTP) (6400 m2 plot area) and Keramat Forest (4800 m2 plot area), Central Lombok District, West Nusa Tenggara Province. The objective of the study was to find out the diversity of tree species and its distribution which can be used for forest area management by the local government and can support the establishment of a Special Economic Region in Mandalika as a place for conservation, research, education and scientific tourism. In the total plot of 1.12 hectare, we recorded 1560 individuals, representing 56 species,  47 genera  and 19 families. All stems ≥ 5 cm in diameter were measured and identified. In the plot of Mt. Tunak NTP, we recorded 992 individuals, representing 41 species, basal area was 11.4 m2, 31.7 m3of estimated bole volume, 78.3 tons biomass, 39.2 tons carbon stock and 143.7 tons CO2 sequestration. Five dominant tree species (trunk diameter ≥ 5 cm) recorded with the highest Importance Value Index (IVI) were Aglaia argentea (IVI= 34.3%), Schoutenia ovata (IVI= 33.3%), Drypetes neglecta (IVI= 25.4%), Glycosmis pentaphylla (IVI= 16.4%) and Grewia koordersiana (IVI= 14.9%). The level of diversity of tree species in the Mt. Tunak NTP was classified into a midle level with a Diversity Index (H')= 3.3, while in the Keramat Forest we recorded 568 individuals, representing 37 species, basal area was 19.9 m2, 51.1 m3 of estimated bole volume, 149.8 tons biomass, 74.9 tons carbon stock and 274.9 tons CO2 sequestration. Five dominant tree species were recorded; Dalbergia latifolia (IVI= 69.4%), Grewia koordersiana (IVI= 34.5%), Schoutenia ovata (IVI= 34.3%), Cupaniopsis macropetala (IVI= 18.2%) and Rhus succedanea (IVI= 14.2%). The level of diversity recorded (H')= 2.9 and classified as midle level. The composition of vegetation in Mt.Tunak NTP and Keramat Forest is different (Jaccard Similarity Index was 39.3%).
Living Longer, Working Smarter, Ageing Well – Ízelítő a konferencián bemutatott kutatásokból - 1.
Authors: Burka Dávid , Vaskövi Ágnes , Vékás Péter
Published: October 2020

A Living Longer, Working Smarter, Ageing Well nemzetközi konferencia a Budapesti Corvinus Egyetem EFOP-3.6.2-16-2017-00017 „Fenntartható, intelligens és befogadó regionális és városi modellek” projekt III/1. Halandó alprojektjének szervezésében valósult meg 2020. május 18-19- én (a COVID-19 veszélyhelyzetre tekintettel online platformon). A konferenciaelőadásokat három szekcióba rendeztük: • az első szekcióban (Ageing, Mortality and Pension) 6 előadást hallgathatott meg a közönség, • a második szekcióban (Labour Market) 3 előadó mutatta be kutatását, végül • a harmadik szekcióban (Well-being) 4 előadás színesítette a két napos programot. Négy meghívott előadó vett részt a konferencián, akik különböző országokból – sőt különböző földrészekről – hoztak érdekes kutatásokat az egyes szekciókba. Az „Ageing, Mortality and Pension” szekcióban hallottuk Ronald Richman, dél-afrikai kutató előadását a Lee-Carter mortalitás előrejelző modell és a gépi tanulás összefüggéseiről, valamint Jan Gogola, cseh-szlovák kutató munkáját a visegrádi országok várható élettartam növekedésének lassulásáról. Jolanta Perek-Białas, lengyel professzor a „Labour Market” szekcióban mutatta be kutatását a hoszszabbodó várható élettartammal együtt járó munkaerőpiaci nehézségekről, idősödő munkavállalók diszkriminációjáról. Ugyanebben a szekcióban hallottuk Arkadiusz Wiśnowski, NagyBritanniában élő lengyel kutató újító tanulmányát az európai migráció becsléséről. Összefoglaló cikkünkben a konferencián elhangzott előadások alapján adunk ízelítőt az egyes témákból, mellyel nem titkolt szándékuk felhívni az olvasó figyelmét előadóink jelen kötetben megjelenő tanulmányaira.
Estudo de viabilidade econômica e financeira para produção artesanal de queijo Cabacinha na região do Vale do Jequitinhonha - MG
Authors: Souza Bruna Randi , Pires Christiano Vieira , Ubaldo Juliana Cristina Sampaio Rigueira , Reis Giann Braune , Trombete Felipe Machado
Published: October 2020

O Queijo Cabacinha é um produto de grande importância na região do Vale do Jequitinhonha-MG, contribuindo para a geração de trabalho e renda para agricultores familiares. Ainda pouco conhecido, é elaborado de forma caseira e não padronizada por grande parte dos agricultores, o que pode comprometer a qualidade e segurança do produto. Nesse sentido, a formalização da produção através de uma agroindústria familiar, respeitando os aspectos técnicos quanto as instalações e processamento, pode ser uma alternativa para a sua produção de forma segura e com qualidade. Esse trabalho objetivou realizar um estudo de viabilidade econômica e financeira de um projeto de agroindústria familiar para a fabricação do queijo artesanal Cabacinha, em condições adequadas de boas práticas de fabricação, visando agregar valor e qualidade a produção local. Foram abordados os aspectos mercadológicos e operacionais da produção, indicando as condições que devem ser respeitadas para as instalações e para o processamento do leite até sua transformação no queijo. No projeto financeiro, foi elaborada uma projeção do fluxo de caixa da empresa para os primeiros anos de atividade, bem como calculados a lucratividade, rentabilidade, Valor Presente Líquido, Taxa Interna de Retorno e o Payback. Pode-se concluir que é viável o investimento para a construção da queijaria artesanal, visando a produção em regime de agroindústria familiar de pequena escala no Vale do Jequitinhonha. A produção irá proporcionar ao produtor rural maior valorização do leite, contribuindo para a produção de um alimento seguro para o consumidor, bem como, respeitando e fortalecendo as tradições locais.