bluuebunny/crossref_metadata_2025 huggingface dataset:
Adaptive resistance to antibiotics in bacteria: a systems biology perspective
Authors: Sandoval‐Motta Santiago , Aldana Maximino
Published: April 2016

Despite all the major breakthroughs in antibiotic development and treatment procedures, there is still no long‐term solution to the bacterial antibiotic resistance problem. Among all the known types of resistance, adaptive resistance (AdR) is particularly inconvenient. This phenotype is known to emerge as a consequence of concentration gradients, as well as contact with subinhibitory concentrations of antibiotics, both known to occur in human patients and livestock. Moreover, AdR has been repeatedly correlated with the appearance of multidrug resistance, although the biological processes behind its emergence and evolution are not well understood. Epigenetic inheritance, population structure and heterogeneity, high mutation rates, gene amplification, efflux pumps, and biofilm formation have all been reported as possible explanations for its development. Nonetheless, these concepts taken independently have not been sufficient to prevent AdR’s fast emergence or to predict its low stability. New strains of resistant pathogens continue to appear, and none of the new approaches used to kill them (mixed antibiotics, sequential treatments, and efflux inhibitors) are completely efficient. With the advent of systems biology and its toolsets, integrative models that combine experimentally known features with computational simulations have significantly improved our understanding of the emergence and evolution of the adaptive‐resistant phenotype. Apart from outlining these findings, we propose that one of the main cornerstones of AdR in bacteria, is the conjunction of two types of mechanisms: one rapidly responding to transient environmental challenges but not very efficient, and another much more effective and specific, but developing on longer time scales. WIREs Syst Biol Med 2016, 8:253–267. doi: 10.1002/wsbm.1335

This article is categorized under:

Analytical and Computational Methods > Computational Methods

Physiology > Organismal Responses to Environment

Biological Mechanisms > Regulatory Biology
Strong coupling of centennial-scale changes of Asian monsoon and soil processes derived from stalagmite δ18O and δ13C records, southern China
Authors: Liu Dianbing , Wang Yongjin , Cheng Hai , Edwards R. Lawrence , Kong Xinggong , Li Ting-Yong
Published: April 2016

The paleoclimate application of speleothem δ13C is influenced by site-specific processes. Here we present four stalagmite δ13C records from two caves in southern China, covering early and late Marine Isotope Stage (MIS) 3 and the Holocene, to investigate the spatio-temporal pattern of calcite δ13C changes and the relationship with Asian monsoon (AM) variability. In each growth period, precessional- to millennial-scale changes are clear in the δ18O record. In contrast, millennial variability is absent in the δ13C record, which characterizes persistent centennial oscillations. However, centennial-scale δ18O variations agree well with those of δ13C, with a larger amplitude in δ13C changes (about twice that of δ18O). This suggests that soil humidity balance associated with regional hydrological circulations is important for these centennial δ13C changes, although evaporation-related kinetic fractionation can induce concurrent enrichments in δ18O and δ13C. In frequency, the detrended δ18O and δ13C records are coupled at a periodicity of about 300 yr during the last glacial period and 150 yr during the Holocene. Those centennial-scale δ13C variations are generally consistent with Greenland temperature variability, indicating a climate response over broad regions. Thus, strong co-variation of δ18O and δ13C records should have a climatic origin, even if it is amplified by kinetic effects.
Daily bread: a novel vehicle for dissemination and evaluation of psychological first aid for families exposed to armed conflict in Syria
Authors: El-Khani A. , Cartwright K. , Redmond A. , Calam R.
Published: April 2016

Background.

Risks to the mental health of children and families exposed to conflict in Syria are of such magnitude that research identifying how best to deliver psychological first aid is urgently required. This study tested the feasibility of a novel approach to large-scale distribution of information and data collection.

Methods.

Routine humanitarian deliveries of bread by a bakery run by a non-governmental organisation (NGO) were used to distribute parenting information leaflets and questionnaires to adults looking after children in conflict zones inside Syria. Study materials were emailed to a project worker in Turkey. Leaflets and questionnaires requesting feedback were transported alongside supplies to a bakery in Syria, and then packed with flatbreads. Three thousand bread-packs were distributed, from three distribution points to which questionnaires were returned, and then taken to Turkey and dispatched to the UK.

Findings.

Notwithstanding delays, 3000 leaflets and questionnaires were successfully distributed over 2 days. Questionnaire return yielded 1783 responses, a 59.5% return rate. Overall ratings of the usefulness of the leaflet were 1060 (59.5%) ‘quite a lot’ and 339 (19.0%) ‘a great deal’. Content analysis was used to code 400 respondent comments. Four themes emerged; positive comments about the leaflet, suggestions for modifications, descriptions of children's needs and the value respondents placed on faith.

Interpretation.

Findings indicate the willingness of NGO staff and volunteers to assist in research, the remarkable willingness of caregivers to respond and the value of brief advice. It demonstrates the scope for using existing humanitarian routes to distribute information and receive feedback even in high-risk settings.
Explaining varying lobbying styles across the Atlantic: an empirical test of the cultural and institutional explanations
Authors: Hanegraaff Marcel , Poletti Arlo , Beyers Jan
Published: April 2016

There is consensus in the literature that policymaking in the United States (US) and Europe generates different lobbying styles. Two explanations for these differences have been developed so far. The first posits that distinct lobbying styles reflect differentpolitical cultures. The second attributes distinct lobbying styles to variation in theinstitutionalcontext in which lobbyists operate. Studies that have analysed lobbying within the US and Europe and assessed the relative importance of these arguments are problematic because both explanations are consistent with observed differences in lobbying style. In this article, we circumvent problems of observational equivalence by focussing on European and American lobbyists who are active in a similar institutional venue – that is, international diplomatic conferences. Relying on evidence collected at World Trade Organization Ministerial Conferences and United Nation Climate Summits, we tested the relevance of alternative explanations for the variation in lobbying styles between European and American lobbyists. Our results give robust support to the institutional argument.
The Public Policy Roots of Women's Increasing College Degree Attainment: The National Defense Education Act of 1958 and the Higher Education Act of 1965
Authors: Rose Deondra
Published: April 2016

How do we explain the steep increase in women's higher educational attainment that began in the mid-twentieth century and has continued, unchecked, in subsequent decades? Although many point to the emergence of feminism and the creation of Title IX in the 1970s as the origins of this trend, I argue that two federal student aid programs—the National Defense Education Act of 1958 and the Higher Education Act of 1965—helped set the stage for women to surpass men as the recipients of bachelor's degrees. Using historical analysis of primary and secondary resources, I present two related case studies that demonstrate the central role that unique political contexts and nondiscriminatory program administration have played in lawmakers' capacity to promote equal opportunity through public policy. This study suggests that women's increasing college degree attainment has important, but frequently overlooked, public policy roots.
Civil Rights, World War II, and U.S. Public Opinion
Authors: White Steven
Published: April 2016

Scholars of American politics often assume World War II liberalized white racial attitudes. This conjecture is generally premised on the existence of an ideological tension between a war against Nazism and the maintenance of white supremacy at home, particularly the Southern system of Jim Crow. A possible relationship between the war and civil rights was also suggested by a range of contemporaneous voices, including academics like Gunnar Myrdal and activists like Walter White and A. Philip Randolph. However, while intuitively plausible, this relationship is generally not well verified empirically. A common flaw is the lack of attention to public opinion polls from the 1940s. Using the best available survey evidence, I argue the war's impact on white racial attitudes is more limited than is often claimed. First, I demonstrate that for whites in the mass public, while there is some evidence of liberalization on issues of racial prejudice, this generally does not extend to policies addressing racial inequities. White opposition to federal anti-lynching legislation actually seems to have increased during the war. Second, there is some evidence of racial moderation among white veterans, relative to their counterparts who did not serve. White veterans were more supportive of anti-lynching legislation in the immediate postwar period, and they offered stronger support for black voting rights in the early 1960s. However, they were not distinguishable on many other issues, including measures of racial prejudice and attitudes toward segregation.
Escherichia coli type III secretion system 2 (ETT2) is widely distributed in avian pathogenic Escherichia coli isolates from Eastern China
Authors: WANG S. , LIU X. , XU X. , ZHAO Y. , YANG D. , HAN X. , TIAN M. , DING C. , PENG D. , YU S.
Published: April 2016

Pathogens utilize type III secretion systems to deliver effector proteins, which facilitate bacterial infections. The Escherichia coli type III secretion system 2 (ETT2) which plays a crucial role in bacterial virulence, is present in the majority of E. coli strains, although ETT2 has undergone widespread mutational attrition. We investigated the distribution and characteristics of ETT2 in avian pathogenic E. coli (APEC) isolates and identified five different ETT2 isoforms, including intact ETT2, in 57·6% (141/245) of the isolates. The ETT2 locus was present in the predominant APEC serotypes O78, O2 and O1. All of the ETT2 loci in the serotype O78 isolates were degenerate, whereas an intact ETT2 locus was mostly present in O1 and O2 serotype strains, which belong to phylogenetic groups B2 and D, respectively. Interestingly, a putative second type III secretion-associated locus (eip locus) was present only in the isolates with an intact ETT2. Moreover, ETT2 was more widely distributed in APEC isolates and exhibited more isoforms compared to ETT2 in human extraintestinal pathogenic E. coli, suggesting that APEC might be a potential risk to human health. However, there was no distinct correlation between ETT2 and other virulence factors in APEC.
ENTREPRENEURS, FINANCIERS, AND BOOM–BUST CYCLES
Authors: Kunieda Takuma , Shibata Akihisa
Published: April 2016

In this paper, a dynamic general equilibrium model with infinitely lived entrepreneurs and financiers is developed to investigate a possible mechanism that explains business cycles and financial crises. The highest growth rate is achievable only if financiers coexist with entrepreneurs, given a certain extent of financial market imperfections. However, if financiers coexist with entrepreneurs, the economy is highly likely to face a financial crisis at certain parameter values. These two-sided implications of the coexistence of entrepreneurs and financiers explain why both instability and high growth are frequently observed in modern economies. Furthermore, our model can obtain countercyclical movements in total factor productivity growth that cannot be explained by the standard real business cycle theory but were observed in the Great Recession of 2007–2008.
THE CYCLICAL BEHAVIOR OF UNEMPLOYMENT AND VACANCIES WITH LOSS OF SKILLS DURING UNEMPLOYMENT
Authors: Ortego-Marti Victor
Published: April 2016

This paper studies the cyclical fluctuations in unemployment and vacancies in a search and matching model in which workers lose skills during periods of unemployment. Firms' profits fluctuate more because aggregate productivity affects the economy's average human capital. Moreover, wages for workers with lower levels of human capital are closer to the value of nonmarket time, leading to more rigid wages. Fluctuations in the vacancy--unemployment ratio are larger than those in the baseline search and matching model and similar to those we observe in the data.
Political Violence in Consolidated Democracies: The Development and Institutionalization of Partisan Violence in Late Colonial Jamaica (1938–62)
Authors: Dawson Andrew
Published: April 2016

Past research suggests that although political violence in mature democracies is rare, it does occasionally occur along ethnic, religious, and/or linguistic lines. Jamaica is an exceptional case in that it is a relatively mature democracy that experiences political violence between demographically similar groups. This article examines the origins of political violence in Jamaica—that is, the conditions that led to its development, intensification, and institutionalization during the late colonial period. Through original archival research, this article supports past findings identifying personality politics, the politicization of race/class divisions, and clientelism as contributing factors to the development of political violence. The research also, however, makes a major new contribution by providing evidence that colonial nonintervention during the early stages of political violence was a crucial factor leading to its escalation and then institutionalization. This finding gives the British colonial state a different and more central role than the extant literature suggests and has broader implications for all democracies.
The Problem of Solidarity in Insurgent Collective Action: The Nore Mutiny of 1797
Authors: Pfaff Steven , Hechter Michael , Corcoran Katie E.
Published: April 2016

How do insurgents engaged in high-risk collective action maintain solidarity when faced with increasing costs and dangers? Based on a combination of process tracing through qualitative evidence and an event-history analysis of a unique data set assembled from naval archives concerning a mass mutiny in the Royal Navy in 1797, this article explains why insurgent solidarity varied among the ships participating in the mutiny. Maintaining solidarity was the key problem that the organizers of the mutiny faced in confronting government repression and inducements for ships’ companies to defect. Solidarity, proxied here as the duration of a ship's company's adherence to the mutiny, relied on techniques used by the mutiny leadership that increased dependence and imposed control over rank-and-file seamen. In particular, mutiny leaders monitored and sanctioned compliance and exploited informational asymmetries to persuade seamen to stand by the insurgency, even as prospects for its success faded.
Political Party Representation and Electoral Politics in England and Wales, 1690–1747
Authors: Bogart Dan
Published: April 2016

The Whig and Tory parties played an important role in British politics in the decades following the Glorious Revolution. This article introduces new data on the political affiliation of all Members of Parliament in England and Wales between 1690 and 1747. The data have numerous applications for research. The focus here is on majority party representation and the electoral politics of constituencies. I show that the Whigs had stronger representation in municipal boroughs with small and narrow electorates, whereas the Tories were stronger in county constituencies and in boroughs with large and more democratic electorates. The Whigs were stronger in the Southeast region and the Tories in Wales and the West Midlands. After the Whig leader, Robert Walpole, became prime minister in 1721 the Whigs lost some presence in their traditional strongholds including counties where the Dissenter population was large. Finally, I incorporate data on electoral contests and show that the majority party generally lost strength in constituencies following contests.
Therapeutenbegleitete und patientengeleitete Exposition bei Panikstörung mit Agoraphobie
Authors: Klan Timo , Persike Malte , Hiller Wolfgang
Published: April 2016

Zusammenfassung. Theoretischer Hintergrund: Die Exposition gilt als hoch wirksames Therapieelement in der Behandlung der Panikstörung mit Agoraphobie. Es ist jedoch weitgehend unklar, wie häufig und mit welchen Effekten verschiedene Modalitäten der Exposition (therapeutenbegleitet vs. patientengeleitet) in der Routineversorgung durchgeführt werden. Fragestellung: Es sollen Häufigkeit und Wirksamkeit der verschiedenen Durchführungsmodalitäten von Exposition in der Routinebehandlung der Panikstörung mit Agoraphobie untersucht werden. Methode: Bei N = 93 konsekutiven Patienten einer Hochschulambulanz mit der Hauptdiagnose Panikstörung mit Agoraphobie wurden Prä-Post-Veränderungen der Angstsymptomatik in Abhängigkeit von verschiedenen Expositionsmodalitäten erfasst. Die Auswirkungen von therapeutenbegleiteter und patientengeleiteter Exposition wurden getrennt für In-vivo-Exposition und interozeptive Exposition (IE) untersucht. Ergebnisse: Mit einer Kombination aus therapeutenbegleiteter und patientengeleiteter Exposition ließen sich tendenziell die besseren Therapieergebnisse erzielen, dieser Effekt ließ sich sowohl für die In-vivo-Exposition als auch für die IE nachweisen. Schlussfolgerung: Nach Möglichkeit sollten In-vivo-Exposition sowie IE sowohl therapeutenbegleitet als auch zusätzlich patientengeleitet (als selbständige Hausaufgabe) durchgeführt werden.
The Development and Initial Validation of the Irrational Performance	Beliefs Inventory (iPBI)
Authors: Turner Martin J. , Allen Mark S. , Slater Matthew J. , Barker Jamie B. , Woodcock Charlotte , Harwood Chris G. , McFayden Ken
Published: April 2016

Abstract. The growing use of Rational Emotive Behavior Therapy (REBT) in performance contexts (e.g., business, sport) has highlighted the absence of a contextually valid and reliable measure of irrational beliefs. This paper reports the development and initial validation of the Irrational Performance Beliefs Inventory (iPBI). The iPBI was developed to provide a validated measure of the four core irrational beliefs of REBT theory. Item development was completed in three stages comprising two expert panels and one novice panel, reducing and refining 176 items to 133. Then, exploratory and confirmatory factor analyses were used to refine the measure and reduce the number of items. A total of 665 business professionals completed the 133-item scale, alongside an established measure of irrational beliefs and a measure of negative emotion. A 28-item measure was developed (the iPBI) that showed an acceptable fit to the four-factor REBT structure. The iPBI correlated well with the established irrational beliefs measure, and with anxiety, depression, and anger, demonstrating concurrent and predictive validity. Further validation efforts are required to assess the validity and reliability of the iPBI in alternative samples in other performance-related contexts.
The Role of Self-Efficacy and Social Support in Improving Life	Satisfaction
Authors: Gayathri Nagarajan , Karthikeyan Parthasarathy
Published: April 2016

Abstract. This paper analyzes the relationship between self-efficacy, work support, family support, and life satisfaction and it also explores the mediating role of work-to-family enrichment and family-to-work enrichment. The participants (n = 568) were from different occupational groups including high technology organizations, hospitals, schools, factories, and government. The correlation analyses and structural equation modeling were performed to explore the relationship existing between the variables. It is found that there exists a positive relationship between self-efficacy, social support, and life satisfaction. Work family enrichment partially mediates the relationship between the studied antecedents and consequences. The work indicates that organizations need to consider ways to not only reduce conflict, but also increase enrichment, which will drive the main outcome variable of overall life satisfaction of the employees.
UV/VUV switch-driven color-reversal effect for Tb-activated phosphors
Authors: Lin Chun Che , Chen Wei-Ting , Chu Cheng-I , Huang Kuan-Wei , Yeh Chiao-Wen , Cheng Bing-Ming , Liu Ru-Shi
Published: April 2016

The remarkable narrow-band emission of trivalent lanthanide-doped phosphors excited by the vacuum ultraviolet (VUV) radiation lines of Xe atoms/Xe2 molecules at 147/172 nm are extensively investigated in the development of plasma display panels and Hg-free fluorescent lamps, which are frequently used in our daily lives. Numerous solid materials, particularly Tb3+-doped oxides, such as silicates, phosphates and borates, are efficient green/blue sources with color-tunable properties. The excitation wavelength and rare earth concentration are usually varied to optimize efficiency and the luminescent properties. However, some underlying mechanisms for the shift in the emission colors remain unclear. The present study shows that a UV/VUV switch systematically controls the change in the phosphor (Ba3Si6O12N2:Tb) photoluminescence from green to blue, resulting in a green emission when the system is excited with UV radiation. However, a blue color is observed when the radiation wavelength shifts to the VUV region. Thus, a configurational coordinate model is proposed for the color-reversal effect. In this model, the dominant radiative decay results in a green emission under low-energy UV excitation from the 5D4 state of the f–f inner-shell transition in the Tb system. However, under high-energy VUV excitation, the state switches into the 5D3 state, which exhibits a blue emission. This mechanism is expected to be generally applicable to Tb-doped phosphors and useful in adjusting the optical properties against well-known cross-relaxation processes by varying the ratio of the green/blue contributions.
Solid–solid phase transitions via melting in metals
Authors: Pogatscher S. , Leutenegger D. , Schawe J. E. K. , Uggowitzer P. J. , Löffler J. F.
Published: April 2016

Observing solid–solid phase transitions in-situ with sufficient temporal and spatial resolution is a great challenge, and is often only possible via computer simulations or in model systems. Recently, a study of polymeric colloidal particles, where the particles mimic atoms, revealed an intermediate liquid state in the transition from one solid to another. While not yet observed there, this finding suggests that such phenomena may also occur in metals and alloys. Here we present experimental evidence for a solid–solid transition via the formation of a metastable liquid in a ‘real’ atomic system. We observe this transition in a bulk glass-forming metallic system in-situ using fast differential scanning calorimetry. We investigate the corresponding transformation kinetics and discuss the underlying thermodynamics. The mechanism is likely to be a feature of many metallic glasses and metals in general, and may provide further insight into phase transition theory.
Scalable salt-templated synthesis of two-dimensional transition metal oxides
Authors: Xiao Xu , Song Huaibing , Lin Shizhe , Zhou Ying , Zhan Xiaojun , Hu Zhimi , Zhang Qi , Sun Jiyu , Yang Bo , Li Tianqi , Jiao Liying , Zhou Jun , Tang Jiang , Gogotsi Yury
Published: April 2016

Two-dimensional atomic crystals, such as two-dimensional oxides, have attracted much attention in energy storage because nearly all of the atoms can be exposed to the electrolyte and involved in redox reactions. However, current strategies are largely limited to intrinsically layered compounds. Here we report a general strategy that uses the surfaces of water-soluble salt crystals as growth templates and is applicable to not only layered compounds but also various transition metal oxides, such as hexagonal-MoO3, MoO2, MnO and hexagonal-WO3. The planar growth is hypothesized to occur via a match between the crystal lattices of the salt and the growing oxide. Restacked two-dimensional hexagonal-MoO3 exhibits high pseudocapacitive performances (for example, 300 F cm−3 in an Al2(SO4)3 electrolyte). The synthesis of various two-dimensional transition metal oxides and the demonstration of high capacitance are expected to enable fundamental studies of dimensionality effects on their properties and facilitate their use in energy storage and other applications.
Ion selectivity of graphene nanopores
Authors: Rollings Ryan C. , Kuan Aaron T. , Golovchenko Jene A.
Published: April 2016

As population growth continues to outpace development of water infrastructure in many countries, desalination (the removal of salts from seawater) at high energy efficiency will likely become a vital source of fresh water. Due to its atomic thinness combined with its mechanical strength, porous graphene may be particularly well-suited for electrodialysis desalination, in which ions are removed under an electric field via ion-selective pores. Here, we show that single graphene nanopores preferentially permit the passage of K+ cations over Cl− anions with selectivity ratios of over 100 and conduct monovalent cations up to 5 times more rapidly than divalent cations. Surprisingly, the observed K+/Cl− selectivity persists in pores even as large as about 20 nm in diameter, suggesting that high throughput, highly selective graphene electrodialysis membranes can be fabricated without the need for subnanometer control over pore size.
Neuroretinal hypoxic signaling in a new preclinical murine model for proliferative diabetic retinopathy
Authors: Wert Katherine J , Mahajan Vinit B , Zhang Lijuan , Yan Yuanqing , Li Yao , Tosi Joaquin , Wei Hsu Chun , Nagasaki Takayuki , Janisch Kerstin M , Grant Maria B , Mahajan MaryAnn , Bassuk Alexander G , Tsang Stephen H
Published: April 2016

Diabetic retinopathy (DR) affects approximately one-third of diabetic patients and, if left untreated, progresses to proliferative DR (PDR) with associated vitreous hemorrhage, retinal detachment, iris neovascularization, glaucoma and irreversible blindness. In vitreous samples of human patients with PDR, we found elevated levels of hypoxia inducible factor 1 alpha (HIF1α). HIFs are transcription factors that promote hypoxia adaptation and have important functional roles in a wide range of ischemic and inflammatory diseases. To recreate the human PDR phenotype for a preclinical animal model, we generated a mouse with neuroretinal-specific loss of the von Hippel Lindau tumor suppressor protein, a protein that targets HIF1α for ubiquitination. We found that the neuroretinal cells in these mice overexpressed HIF1α and developed severe, irreversible ischemic retinopathy that has features of human PDR. Rapid progression of retinopathy in these mutant mice should facilitate the evaluation of therapeutic agents for ischemic and inflammatory blinding disorders. In addition, this model system can be used to manipulate the modulation of the hypoxia signaling pathways, for the treatment of non-ocular ischemic and inflammatory disorders.
Exceptional points and asymmetric mode conversion in quasi-guided dual-mode optical waveguides
Authors: Ghosh S. N. , Chong Y. D.
Published: April 2016

Non-Hermitian systems host unconventional physical effects that be used to design new optical devices. We study a non-Hermitian system consisting of 1D planar optical waveguides with suitable amount of simultaneous gain and loss. The parameter space contains an exceptional point, which can be accessed by varying the transverse gain and loss profile. When light propagates through the waveguide structure, the output mode is independent of the choice of input mode. This “asymmetric mode conversion” phenomenon can be explained by the swapping of mode identities in the vicinity of the exceptional point, together with the failure of adiabatic evolution in non-Hermitian systems.
Terahertz and mid-infrared reflectance of epitaxial graphene
Authors: Santos Cristiane N. , Joucken Frédéric , De Sousa Meneses Domingos , Echegut Patrick , Campos-Delgado Jessica , Louette Pierre , Raskin Jean-Pierre , Hackens Benoit
Published: April 2016

Graphene has emerged as a promising material for infrared (IR) photodetectors and plasmonics. In this context, wafer scale epitaxial graphene on SiC is of great interest in a variety of applications in optics and nanoelectronics. Here we present IR reflectance spectroscopy of graphene grown epitaxially on the C-face of 6H-SiC over a broad optical range, from terahertz (THz) to mid-infrared (MIR). Contrary to the transmittance, reflectance measurements are not hampered by the transmission window of the substrate and in particular by the SiC Reststrahlen band in the MIR. This allows us to present IR reflectance data exhibiting a continuous evolution from the regime of intraband to interband charge carrier transitions. A consistent and simultaneous analysis of the contributions from both transitions to the optical response yields precise information on the carrier dynamics and the number of layers. The properties of the graphene layers derived from IR reflection spectroscopy are corroborated by other techniques (micro-Raman and X-ray photoelectron spectroscopies, transport measurements). Moreover, we also present MIR microscopy mapping, showing that spatially-resolved information can be gathered, giving indications on the sample homogeneity. Our work paves the way for a still scarcely explored field of epitaxial graphene-based THz and MIR optical devices.
Perceptual drifts of real and artificial limbs in the rubber hand illusion
Authors: Fuchs Xaver , Riemer Martin , Diers Martin , Flor Herta , Trojan Jörg
Published: April 2016

In the rubber hand illusion (RHI), transient embodiment of an artificial hand is induced. An often-used indicator for this effect is the “proprioceptive drift”, a localization bias of the real hand towards the artificial hand. This measure suggests that the real hand is attracted by the artificial hand. Principles of multisensory integration, however, rather suggest that conflicting sensory information is combined in a “compromise” fashion and that hands should rather be attracted towards each other. Here, we used a new variant of the RHI paradigm in which participants pointed at the artificial hand. Our results indicate that the perceived positions of the real and artificial hand converge towards each other: in addition to the well-known drift of the real hand towards the artificial hand, we also found an opposite drift of the artificial hand towards the real hand. Our results contradict the notion of perceptual substitution of the real hand by the artificial hand. Rather, they are in line with the view that vision and proprioception are fused into an intermediate percept. This is further evidence that the perception of our body is a flexible multisensory construction that is based on integration principles.
Effects of low dose estrogen therapy on the vaginal microbiomes of women with atrophic vaginitis
Authors: Shen Jian , Song Ning , Williams Christopher J. , Brown Celeste J. , Yan Zheng , Xu Chen , Forney Larry J.
Published: April 2016

Atrophic vaginitis (AV) is common in postmenopausal women, but its causes are not well understood. The symptoms, which include vaginal itching, burning, dryness, irritation, and dyspareunia, can usually be alleviated by low doses of estrogen given orally or locally. Regrettably, the composition of vaginal bacterial communities in women with AV have not been fully characterized and little is known as to how these communities change over time in response to hormonal therapy. In the present intervention study we determined the response of vaginal bacterial communities in postmenopausal women with AV to low-dose estrogen therapy. The changes in community composition in response to hormonal therapy were rapid and typified by significant increases in the relative abundance of Lactobacillus spp. that were mirrored by a decreased relative abundance of Gardnerella. These changes were paralleled by a significant four-fold increase in serum estradiol levels and decreased vaginal pH, as well as nearly a two-fold increase in the Vaginal Maturation Index (VMI). The results suggest that after menopause a vaginal microbiota dominated by species of Lactobacillus may have a beneficial role in the maintenance of health and these findings that could lead to new strategies to protect postmenopausal women from AV.
Erratum: Involvement of hormones in olfactory imprinting and homing in chum salmon
Authors: Ueda Hiroshi , Nakamura Shingo , Nakamura Taro , Inada Kaoru , Okubo Takashi , Furukawa Naohiro , Murakami Reiichi , Tsuchida Shigeo , Zohar Yonathan , Konno Kotaro , Watanabe Masahiko
Published: April 2016

Scientific Reports 6: Article number: 21102; published online: 16 February 2016; updated: 22 April 2016 In the Supplementary Information file originally published with this Article, the lower part of Fig. S1 ‘sGnRH-II’ was incorrectly labelled ‘GnRH-II’. This error has been corrected in the Supplementary Information that now accompanies the Article.
Correction: Corrigendum: Masculinised Behaviour of XY Females in a Mammal with Naturally Occurring Sex Reversal
Authors: Saunders Paul A. , Franco Thomas , Sottas Camille , Maurice Tangui , Ganem Guila , Veyrunes Frédéric
Published: April 2016

Scientific Reports 6: Article number: 22881; published online: 11 March 2016; updated: 22 April 2016 The original version of this Article contained an error in the title of the paper, where the word “Occurring” was incorrectly given as “Occuring”. In addition, the Acknowledgements section was incomplete.
Oxygen-enabled control of Dzyaloshinskii-Moriya Interaction in ultra-thin magnetic films
Authors: Belabbes Abderrezak , Bihlmayer Gustav , Blügel Stefan , Manchon Aurélien
Published: April 2016

The search for chiral magnetic textures in systems lacking spatial inversion symmetry has attracted a massive amount of interest in the recent years with the real space observation of novel exotic magnetic phases such as skyrmions lattices, but also domain walls and spin spirals with a defined chirality. The electrical control of these textures offers thrilling perspectives in terms of fast and robust ultrahigh density data manipulation. A powerful ingredient commonly used to stabilize chiral magnetic states is the so-called Dzyaloshinskii-Moriya interaction (DMI) arising from spin-orbit coupling in inversion asymmetric magnets. Such a large antisymmetric exchange has been obtained at interfaces between heavy metals and transition metal ferromagnets, resulting in spin spirals and nanoskyrmion lattices. Here, using relativistic first-principles calculations, we demonstrate that the magnitude and sign of DMI can be entirely controlled by tuning the oxygen coverage of the magnetic film, therefore enabling the smart design of chiral magnetism in ultra-thin films. We anticipate that these results extend to other electronegative ions and suggest the possibility of electrical tuning of exotic magnetic phases.
Laser-synthesized oxide-passivated bright Si quantum dots for bioimaging
Authors: Gongalsky M. B. , Osminkina L. A. , Pereira A. , Manankov A. A. , Fedorenko A. A. , Vasiliev A. N. , Solovyev V. V. , Kudryavtsev A. A. , Sentis M. , Kabashin A. V. , Timoshenko V. Yu.
Published: April 2016

Crystalline silicon (Si) nanoparticles present an extremely promising object for bioimaging based on photoluminescence (PL) in the visible and near-infrared spectral regions, but their efficient PL emission in aqueous suspension is typically observed after wet chemistry procedures leading to residual toxicity issues. Here, we introduce ultrapure laser-synthesized Si-based quantum dots (QDs), which are water-dispersible and exhibit bright exciton PL in the window of relative tissue transparency near 800 nm. Based on the laser ablation of crystalline Si targets in gaseous helium, followed by ultrasound-assisted dispersion of the deposited films in physiological saline, the proposed method avoids any toxic by-products during the synthesis. We demonstrate efficient contrast of the Si QDs in living cells by following the exciton PL. We also show that the prepared QDs do not provoke any cytoxicity effects while penetrating into the cells and efficiently accumulating near the cell membrane and in the cytoplasm. Combined with the possibility of enabling parallel therapeutic channels, ultrapure laser-synthesized Si nanostructures present unique object for cancer theranostic applications.
Crystal Structures and Binding Dynamics of Odorant-Binding Protein 3 from two aphid species Megoura viciae and Nasonovia ribisnigri
Authors: Northey Tom , Venthur Herbert , De Biasio Filomena , Chauviac Francois-Xavier , Cole Ambrose , Ribeiro Karlos Antonio Lisboa , Grossi Gerarda , Falabella Patrizia , Field Linda M. , Keep Nicholas H. , Zhou Jing-Jiang
Published: April 2016

Aphids use chemical cues to locate hosts and find mates. The vetch aphidMegoura viciaefeeds exclusively on the Fabaceae, whereas the currant-lettuce aphidNasonovia ribisnigrialternates hosts between the Grossulariaceae and Asteraceae. Both species use alarm pheromones to warn of dangers. ForN. ribisnigrithis pheromone is a single component (E)-β-farnesene butM. viciaeuses a mixture of (E)-β-farnesene, (−)-α-pinene, β-pinene, and limonene. Odorant-binding proteins (OBP) are believed to capture and transport such semiochemicals to their receptors. Here, we report the first aphid OBP crystal structures and examine their molecular interactions with the alarm pheromone components. Our study reveals some unique structural features: 1) the lack of an internal ligand binding site; 2) a striking groove in the surface of the proteins as a putative binding site; 3) the N-terminus rather than the C-terminus occupies the site closing off the conventional OBP pocket. The results from fluorescent binding assays, molecular docking and dynamics demonstrate that OBP3 fromM. viciaecan bind to all four alarm pheromone components and the differential ligand binding between these very similar OBP3s from the two aphid species is determined mainly by the direct π-π interactions between ligands and the aromatic residues of OBP3s in the binding pocket.
Multispectral imaging using a single bucket detector
Authors: Bian Liheng , Suo Jinli , Situ Guohai , Li Ziwei , Fan Jingtao , Chen Feng , Dai Qionghai
Published: April 2016

Existing multispectral imagers mostly use available array sensors to separately measure 2D data slices in a 3D spatial-spectral data cube. Thus they suffer from low photon efficiency, limited spectrum range and high cost. To address these issues, we propose to conduct multispectral imaging using a single bucket detector, to take full advantage of its high sensitivity, wide spectrum range, low cost, small size and light weight. Technically, utilizing the detector’s fast response, a scene’s 3D spatial-spectral information is multiplexed into a dense 1D measurement sequence and then demultiplexed computationally under the single pixel imaging scheme. A proof-of-concept setup is built to capture multispectral data of 64 pixels × 64 pixels × 10 wavelength bands ranging from 450 nm to 650 nm, with the acquisition time being 1 minute. The imaging scheme holds great potentials for various low light and airborne applications and can be easily manufactured as production-volume portable multispectral imagers.
Ultra-sensitive Magnetic Microscopy with an Optically Pumped Magnetometer
Authors: Kim Young Jin , Savukov Igor
Published: April 2016

Optically pumped magnetometers (OPMs) based on lasers and alkali-metal vapor cells are currently the most sensitive non-cryogenic magnetic field sensors. Many applications in neuroscience and other fields require high-resolution, high-sensitivity magnetic microscopic measurements. In order to meet this demand we combined a cm-size spin-exchange relaxation-free (SERF) OPM and flux guides (FGs) to realize an ultra-sensitive FG-OPM magnetic microscope. The FGs serve to transmit the target magnetic flux to the OPM thus improving both the resolution and sensitivity to small magnetic objects. We investigated the performance of the FG-OPM device using experimental and numerical methods and demonstrated that an optimized device can achieve a unique combination of high resolution (80 μm) and high sensitivity (8.1 pT/"Equation missing"). In addition, we also performed numerical calculations of the magnetic field distribution in the FGs to estimate the magnetic noise originating from the domain fluctuations in the material of the FGs. We anticipate many applications of the FG-OPM device such as the detection of micro-biological magnetic fields; the detection of magnetic nano-particles; and non-destructive testing. From our theoretical estimate, an FG-OPM could detect the magnetic field of a single neuron, which would be an important milestone in neuroscience.
Fabrication of Radially Symmetric Graded Porous Silicon using a Novel Cell Design
Authors: Zhao Mingrui , Keswani Manish
Published: April 2016

A contactless method using a novel design of the experimental cell for formation of porous silicon with morphological gradient is reported. Fabricated porous silicon layers show a large distribution in porosity, pore size and depth along the radius of the samples. Symmetrical arrangements of morphology gradient were successfully formulated radially on porous films and the formation was attributed to decreasing current density radially inward on the silicon surface exposed to Triton® X-100 containing HF based etchant solution. Increasing the surfactant concentration increases the pore depth gradient but has a reverse effect on the pore size distribution. Interestingly, when dimethyl sulfoxide was used instead of Triton® X-100 in the etchant solution, no such morphological gradients were observed and a homogeneous porous film was formed.
Neutrophil Elastase-mediated proteolysis activates the anti-inflammatory cytokine IL-36 Receptor antagonist
Authors: Macleod Tom , Doble Rosella , McGonagle Dennis , Wasson Christopher W. , Alase Adewonuola , Stacey Martin , Wittmann Miriam
Published: April 2016

The interleukin-36 receptor antagonist (IL-36Ra) which regulates IL-36α, -β and -γ is linked to psoriatic inflammation, especially loss-of-function mutations in pustular psoriasis subtypes. As observed with other IL-1 superfamily proteins, the IL-36 members require N-terminal cleavage for full biological activity but the mechanisms of IL-36Ra activation remain poorly defined. Using different blood leukocyte and skin resident cell preparations and recombinant proteins, we have identified that neutrophil elastase, but not other neutrophil derived proteases, cleaves IL-36Ra into its highly active antagonistic form. The activity of this processed form of IL-36Ra was confirmed in human primary dermal fibroblasts and keratinocytes and in skin equivalents. A significant dose dependent reduction of IL-36γ induced IL-8 and chemokine ligand 20 (CCL20) levels were detected following addition of the cleaved IL-36Ra compared to full length IL-36Ra. By activating IL-36Ra, the neutrophil derived protease can inhibit IL-36 induced chemokine production, including IL-8 and CCL20 and reduce further inflammatory cell infiltration. These findings strongly indicate neutrophil elastase to be a key enzyme in the biological function of IL-36Ra and that neutrophils can play a regulatory role in psoriatic inflammation with regard to balancing the pro-inflammatory activity of IL-36.
Randomly Distributed Fabry-Pérot-type Metal Nanowire Resonators and Their Lasing Action
Authors: Kwon Kyungmok , Jung Youngho , Kim Minkyung , Shim Jaeho , Yu Kyoungsik
Published: April 2016

Optical feedback mechanisms are often obtained from well-defined resonator structures fabricated by top-down processes. Here, we demonstrate that two-dimensional networks of metallic nanowires dispersed on the semiconductor slab can provide strong in-plane optical feedback and, thus, form randomly-distributed Fabry-Pérot-type resonators that can achieve multi- or single-mode lasing action in the near infrared wavelengths. Albeit with their subwavelength-scale cross-sections and uncontrolled inter-nanowire distances, a cluster of nearly parallel metal nanowires acts as an effective in-situ reflector for the semiconductor-metal slab waveguide modes for coherent optical feedback in the lateral direction. Fabry-Pérot type resonance can be readily developed by a pair of such clusters coincidentally formed in the solution-processed random nanowire network. Our low-cost and large-area approach for opportunistic random cavity formation would open a new pathway for integrated planar light sources for low-coherence imaging and sensing applications.
Arbuscular mycorrhizal fungi in soil and roots respond differently to phosphorus inputs in an intensively managed calcareous agricultural soil
Authors: Liu Wei , Zhang Yunlong , Jiang Shanshan , Deng Yan , Christie Peter , Murray Philip J. , Li Xiaolin , Zhang Junling
Published: April 2016

Understanding the diversity and community structure of arbuscular mycorrhizal fungi (AMF) is important for potentially optimizing their role in mining phosphorus (P) in agricultural ecosystems. Here, we conduct a comprehensive study to investigate the vertical distribution of AMF in a calcareous field and their temporal structure in maize-roots with fertilizer P application over a three-year period. The results showed that soil available-P response to P fertilization but maize yields did not. Phosphorus fertilization had no-significant effect on richness of AMF except at greater soil-depths. High P-supply reduced root colonization while optimum-P tended to increase colonization and fungal richness on all sampling occasions. Crop phenology might override P-supply in determining the community composition of active root inhabiting fungi. Significant differences in the community structure of soil AMF were observed between the controls and P treatments in surface soil and the community shift was attributable mainly to available-P, N/P and pH. Vertical distribution was related mainly to soil electrical conductivity and Na content. Our results indicate that the structure of AMF community assemblages is correlated with P fertilization, soil depth and crop phenology. Importantly, phosphorus management must be integrated with other agricultural-practices to ensure the sustainability of agricultural production in salinized soils.