bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy of the Piperidine Nitroxide 4-MethoxyTEMPO in Ameliorating Serum Amyloid A-Mediated Vascular Inflammation
Authors: Martin Nathan J. , Chami Belal , Vallejo Abigail , Mojadadi Albaraa A. , Witting Paul K. , Ahmad Gulfam
Published: April 2021

Intracellular redox imbalance in endothelial cells (EC) can lead to endothelial dysfunction, which underpins cardiovascular diseases (CVD). The acute phase serum amyloid A (SAA) elicits inflammation through stimulating production of reactive oxygen species (ROS). The cyclic nitroxide 4-MethoxyTEMPO (4-MetT) is a superoxide dismutase mimetic that suppresses oxidant formation and inflammation. The aim of this study was to investigate whether 4-MetT inhibits SAA-mediated activation of cultured primary human aortic EC (HAEC). Co-incubating cells with 4-MetT inhibited SAA-mediated increases in adhesion molecules (VCAM-1, ICAM-1, E-selectin, and JAM-C). Pre-treatment of cells with 4-MetT mitigated SAA-mediated increases in transcriptionally activated NF-κB-p65 and P120 Catenin (a stabilizer of Cadherin expression). Mitochondrial respiration and ROS generation (mtROS) were adversely affected by SAA with decreased respiratory reserve capacity, elevated maximal respiration and proton leakage all characteristic of SAA-treated HAEC. This altered respiration manifested as a loss of mitochondrial membrane potential (confirmed by a decrease in TMRM fluorescence), and increased mtROS production as assessed with MitoSox Red. These SAA-linked impacts on mitochondria were mitigated by 4-MetT resulting in restoration of HAEC nitric oxide bioavailability as confirmed by assessing cyclic guanosine monophosphate (cGMP) levels. Thus, 4-MetT ameliorates SAA-mediated endothelial dysfunction through normalising EC redox homeostasis. Subject to further validation in in vivo settings; these outcomes suggest its potential as a therapeutic in the setting of cardiovascular pathologies where elevated SAA and endothelial dysfunction is linked to enhanced CVD.
Immune-Mediated Drug-Induced Liver Injury: Immunogenetics and Experimental Models
Authors: Gerussi Alessio , Natalini Ambra , Antonangeli Fabrizio , Mancuso Clara , Agostinetto Elisa , Barisani Donatella , Di Rosa Francesca , Andrade Raul , Invernizzi Pietro
Published: April 2021

Drug-induced liver injury (DILI) is a challenging clinical event in medicine, particularly because of its ability to present with a variety of phenotypes including that of autoimmune hepatitis or other immune mediated liver injuries. Limited diagnostic and therapeutic tools are available, mostly because its pathogenesis has remained poorly understood for decades. The recent scientific and technological advancements in genomics and immunology are paving the way for a better understanding of the molecular aspects of DILI. This review provides an updated overview of the genetic predisposition and immunological mechanisms behind the pathogenesis of DILI and presents the state-of-the-art experimental models to study DILI at the pre-clinical level.
Cytogenetic Analysis, Heterochromatin Characterization and Location of the rDNA Genes of Hycleus scutellatus (Coleoptera, Meloidae); A Species with an Unexpected High Number of rDNA Clusters
Authors: Ruiz-Torres Laura , Mora Pablo , Ruiz-Mena Areli , Vela Jesús , Mancebo Francisco J. , Montiel Eugenia E. , Palomeque Teresa , Lorite Pedro
Published: April 2021

Meloidae are commonly known as blister beetles, so called for the secretion of cantharidin, a toxic substance that causes irritation and blistering. There has been a recent increase in the interest of the cantharidin anticancer potential of this insect group. Cytogenetic and molecular data in this group are scarce. In this study, we performed a karyotype analysis of Hycleus scutellatus, an endemic species of the Iberian Peninsula. We determined its chromosome number, 2n = 20, as well as the presence of the X and Y sex chromosomes. In addition to a karyotype analysis, we carried out DAPI staining. By fluorescence in situ hybridization we mapped the rDNA clusters on 12 different chromosomes. Compared to others, this species shows an unusually high number of chromosomes carrying rDNA. This is one of the highest numbers of rDNA sites found in the Polyphaga suborder (Coleoptera). Additionally, we isolated a satellite DNA family (Hyscu-H), which was located within the pericentromeric regions of all chromosomes, including the sex chromosomes. The results suggest that Hyscu-H is likely to be one of the most abundant satellite DNA repeats in H. scutellatus.
Real World Performance Evaluation of Transcatheter Aortic Valve Implantation
Authors: Pesarini Gabriele , Venturi Gabriele , Tavella Domenico , Gottin Leonardo , Lunardi Mattia , Mirandola Elena , Onorati Francesco , Faggian Giuseppe , Ribichini Flavio
Published: April 2021

Background: The aim of this research is to describe the performance over time of transcatheter aortic valve implantations (TAVIs) in a high-volume center with a contemporary, real-world population. Methods: Patients referred for TAVIs at the University Hospital of Verona were prospectively enrolled. By cumulative sum failures analysis (CUSUM), procedural-control curves for standardized combined endpoints—as defined by the Valve Academic Research Consortium-2 (VARC-2)—were calculated and analyzed over time. Acceptable and unacceptable limits were derived from recent studies on TAVI in intermediate and low-risk patients to fit the higher required standards for current indications. Results: A total of 910 patients were included. Baseline risk scores significantly reduced over time. Complete procedural control was obtained after approximately 125 and 190 cases for device success and early safety standardized combined endpoints, respectively. High risk patients (STS ≥ 8) had poorer outcomes, especially in terms of VARC-2 clinical efficacy, and required a higher case load to maintain in-control and proficient procedures. Clinically relevant single endpoints were all influenced by operator’s experience as well. Conclusions: Quality-control analysis for contemporary TAVI interventions based on standardized endpoints suggests the need for relevant operator’s experience to achieve and maintain optimal clinical results, especially in higher-risk subjects.
Individual and Group Response of Treatment with Ivacaftor on Airway and Gut Microbiota in People with CF and a S1251N Mutation
Authors: Kristensen Maartje I. , de Winter-de Groot Karin M. , Berkers Gitte , Chu Mei Ling J. N. , Arp Kayleigh , Ghijsen Sophie , Heijerman Harry G. M. , Arets Hubertus G. M. , Majoor Christof J. , Janssens Hettie M. , van der Meer Renske , Bogaert Debby , van der Ent Cornelis K.
Published: April 2021

Ivacaftor has been shown to restore the functionality of the S1251N (also known as c.3752G>A) mutated CFTR, which may cause alterations in both airway and gut physiology and micro-environment, resulting in a change of microbiota in these organs. The aim of the present study was to analyze the effects of ivacaftor on the microbial community composition of both airway and gut in subjects with CF carrying one S1251N mutation, using a 16S rRNA gene-based sequencing approach. In 16 subjects with CF, repetitive samples from airways and gut were collected just before, and 2 months after, and, for 8 patients, also 9 and 12 months after, start of ivacaftor. 16S rRNA based sequencing identified 344 operational taxonomical units (OTUs) in a total of 139 samples (35 nasopharyngeal, 39 oropharyngeal, 29 sputum, and 36 fecal samples). Ivacaftor significantly enhanced bacterial diversity and overall microbiota composition in the gut (p < 0.01). There were no significant changes in the overall microbial composition and alpha diversity in upper and lower airways of these patients after ivacaftor treatment. Treatment with ivacaftor induces changes in gut microbiota whereas airway microbiota do not change significantly over time.
Highly Cancellous Titanium Alloy (TiAl6V4) Surfaces on Three-Dimensionally Printed, Custom-Made Intercalary Tibia Prostheses: Promising Short- to Intermediate-Term Results
Authors: Guder Wiebke K. , Hardes Jendrik , Nottrott Markus , Podleska Lars E. , Streitbürger Arne
Published: April 2021

Custom-made, three-dimensionally-printed (3D) bone prostheses gain increasing importance in the reconstruction of bone defects after musculoskeletal tumor resections. They may allow preservation of little remaining bone stock and ensure joint or limb salvage. However, we believe that by constructing anatomy-imitating implants with highly cancellous titanium alloy (TiAl6V4) surfaces using 3D printing technology, further benefits such as functional enhancement and reduction of complications may be achieved. We present a case series of four patients reconstructed using custom-made, 3D-printed intercalary monobloc tibia prostheses treated between 2016 and 2020. The mean patient age at operation was 30 years. Tumor resections were performed for Ewing sarcoma (n = 2), high-grade undifferentiated pleomorphic bone sarcoma (n = 1) and adamantinoma (n = 1). Mean resection length was 17.5 cm and mean operation time 147 min. All patients achieved full weight-bearing and limb salvage at a mean follow-up of 21.25 months. One patient developed a non-union at the proximal bone-implant interface. Alteration of implant design prevented non-union in later patients. Mean MSTS and TESS scores were 23.5 and 88. 3D-printed, custom-made intercalary tibia prostheses achieved joint and limb salvage in this case series despite high, published complication rates for biological and endoprosthetic reconstructions of the diaphyseal and distal tibia. Ingrowth of soft tissues into the highly cancellous implant surface structure reduces dead space, enhances function, and appears promising in reducing complication rates.
RNA-seq Characterization of Sex-Differences in Adipose Tissue of Obesity Affected Patients: Computational Analysis of Differentially Expressed Coding and Non-Coding RNAs
Authors: Rey Federica , Messa Letizia , Pandini Cecilia , Maghraby Erika , Barzaghini Bianca , Garofalo Maria , Micheletto Giancarlo , Raimondi Manuela Teresa , Bertoli Simona , Cereda Cristina , Zuccotti Gian Vincenzo , Cancello Raffaella , Carelli Stephana
Published: April 2021

Obesity is a multifactorial disease presenting sex-related differences including adipocyte functions, sex hormone effects, genetics, and metabolic inflammation. These can influence individuals’ risk for metabolic dysfunctions, with an urgent need to perform sex-based analysis to improve prevention, treatment, and rehabilitation programs. This research work is aimed at characterizing the transcriptional differences present in subcutaneous adipose tissue (SAT) of five obesity affected men versus five obesity affected women, with an additional focus on the role of long non-coding RNAs. Through RNA-sequencing, we highlighted the presence of both coding and non-coding differentially expressed RNAs, and with numerous computational analyses we identified the processes in which these genes are implicated, along with their role in co-morbidities development. We report 51 differentially expressed transcripts, 32 of which were coding genes and 19 were non-coding. Using the WGCNA R package (Weighted Correlation Network Analysis, version 1.70-3), we describe the interactions between coding and non-coding RNAs, and the non-coding RNAs association with the insurgence of specific diseases, such as cancer development, neurodegenerative diseases, and schizophrenia. In conclusion, our work highlights a specific gender sex-related transcriptional signature in the SAT of obesity affected patients.
Risk Management Practices by South African Universities: An Annual Report Disclosure Analysis
Authors: Sityata Inga , Botha Lise , Dubihlela Job
Published: April 2021

This paper assesses risk management practices at South African universities by analyzing the extent of risk management disclosure recommended by King IV and the level of risk governance maturity. This study was motivated by #Feesmustfall disruptions, which pointed to the lack of effective risk management, preparedness for volatility and increased scrutiny by stakeholders. A qualitative content analysis using a risk disclosure checklist was conducted on 18 annual reports and analyzed using an exploratory research design. The results revealed that over 80% of the sampled South African universities have disclosed most of their risk management practices, showing an improved disclosure due to King IV’s “apply and explain” philosophy as introduced in 2016. However, there were areas of improvement identified, such as: defining and approval of risk appetites and tolerance; development and implementation of business continuity plans; confirming the unpreparedness for volatility; annual revision of policies; and integration of risk management into the culture and daily activities of the university. This paper builds upon previous studies that highlighted a lack of detailed disclosures in South African organizations’ annual reports. This study also provides interesting insights into the impact of social events on organizational practices and supports the notion that legislative accounting practices should echo stakeholders and societal expectations.
Two Decades of Evolution of Our Understanding of the Transient Receptor Potential Melastatin 2 (TRPM2) Cation Channel
Authors: Szollosi Andras
Published: April 2021

The transient receptor potential melastatin (TRPM) family belongs to the superfamily of TRP ion channels. It consists of eight family members that are involved in a plethora of cellular functions. TRPM2 is a homotetrameric Ca2+-permeable cation channel activated upon oxidative stress and is important, among others, for body heat control, immune cell activation and insulin secretion. Invertebrate TRPM2 proteins are channel enzymes; they hydrolyze the activating ligand, ADP-ribose, which is likely important for functional regulation. Since its cloning in 1998, the understanding of the biophysical properties of the channel has greatly advanced due to a vast number of structure–function studies. The physiological regulators of the channel have been identified and characterized in cell-free systems. In the wake of the recent structural biochemistry revolution, several TRPM2 cryo-EM structures have been published. These structures have helped to understand the general features of the channel, but at the same time have revealed unexplained mechanistic differences among channel orthologues. The present review aims at depicting the major research lines in TRPM2 structure-function. It discusses biophysical properties of the pore and the mode of action of direct channel effectors, and interprets these functional properties on the basis of recent three-dimensional structural models.
Human Brain Lipidomics: Utilities of Chloride Adducts in Flow Injection Analysis
Authors: Wood Paul L. , Hauther Kathleen A. , Scarborough Jon H. , Craney Dustin J. , Dudzik Beatrix , Cebak John E. , Woltjer Randall L.
Published: April 2021

Ceramides have been implicated in a number of disease processes. However, current means of evaluation with flow infusion analysis (FIA) have been limited primarily due to poor sensitivity within our high-resolution mass spectrometry lipidomics analytical platform. To circumvent this deficiency, we investigated the potential of chloride adducts as an alternative method to improve sensitivity with electrospray ionization. Chloride adducts of ceramides and ceramide subfamilies provided 2- to 50-fold increases in sensitivity both with analytical standards and biological samples. Chloride adducts of a number of other lipids with reactive hydroxy groups were also enhanced. For example, monogalactosyl diacylglycerols (MGDGs), extracted from frontal lobe cortical gray and subcortical white matter of cognitively intact subjects, were not detected as ammonium adducts but were readily detected as chloride adducts. Hydroxy lipids demonstrate a high level of specificity in that phosphoglycerols and phosphoinositols do not form chloride adducts. In the case of choline glycerophospholipids, the fatty acid substituents of these lipids could be monitored by MS2 of the chloride adducts. Monitoring the chloride adducts of a number of key lipids offers enhanced sensitivity and specificity with FIA. In the case of glycerophosphocholines, the chloride adducts also allow determination of fatty acid substituents. The chloride adducts of lipids possessing electrophilic hydrogens of hydroxyl groups provide significant increases in sensitivity. In the case of glycerophosphocholines, chloride attachment to the quaternary ammonium group generates a dominant anion, which provides the identities of the fatty acid substituents under MS2 conditions.
Significance of Slippage and Electric Field in Mucociliary Transport of Biomagnetic Fluid
Authors: Munawar Sufian
Published: April 2021

Shear stress at the cilia wall is considered as an imperative factor that affects the efficiency of cilia beatings as it describes the momentum transfer between the fluid and the cilia. We consider a visco-inelastic Prandtl fluid in a ciliated channel under electro-osmotic pumping and the slippage effect at cilia surface. Cilia beating is responsible for the stimulation of the flow in the channel. Evenly distributed cilia tend to move in a coordinated rhythm to mobilize propulsive metachronal waves along the channel surface by achieving elliptic trajectory movements in the flow direction. After using lubrication approximations, the governing equations are solved by the perturbation method. The pressure rise per metachronal wavelength is obtained by numerically integrating the expression. The effects of the physical parameters of interest on various flow quantities, such as velocity, pressure gradient, pressure rise, stream function, and shear stress at the ciliated wall, are discussed through graphs. The analysis reveals that the axial velocity is enhanced by escalating the Helmholtz–Smoluchowski velocity and the electro-osmosis effects near the elastic wall. The shear stress at the ciliated boundary elevates with an increase in the cilia length and the eccentricity of the cilia structure.
Influence of Sintering Temperature of Kaolin, Slag, and Fly Ash Geopolymers on the Microstructure, Phase Analysis, and Electrical Conductivity
Authors: Zulkifli Nur Nadiah Izzati , Abdullah Mohd Mustafa Al Bakri , Przybył Anna , Pietrusiewicz Paweł , Salleh Mohd Arif Anuar Mohd , Aziz Ikmal Hakem , Kwiatkowski Dariusz , Gacek Marcin , Gucwa Marek , Chaiprapa Jitrin
Published: April 2021

This paper clarified the microstructural element distribution and electrical conductivity changes of kaolin, fly ash, and slag geopolymer at 900 °C. The surface microstructure analysis showed the development in surface densification within the geopolymer when in contact with sintering temperature. It was found that the electrical conductivity was majorly influenced by the existence of the crystalline phase within the geopolymer sample. The highest electrical conductivity (8.3 × 10−4 Ωm−1) was delivered by slag geopolymer due to the crystalline mineral of gehlenite (3Ca2Al2SiO7). Using synchrotron radiation X-ray fluorescence, the high concentration Ca boundaries revealed the appearance of gehlenite crystallisation, which was believed to contribute to development of denser microstructure and electrical conductivity.
The Influence of Age Hardening and Shot Peening on the Surface Properties of 7075 Aluminium Alloy
Authors: Žagar Sebastjan , Markoli Boštjan , Naglič Iztok , Šturm Roman
Published: April 2021

The present study investigates the effect of shot peening (SP) on the mechanical properties and surface roughness of 7075 aluminum alloy during different stages and conditions of heat treatment. The mechanical properties were determined by measuring Vickers microhardness profiles and residual stress profiles, while the amount of alloying elements present in the solid solution of the samples under different heat treatment conditions was determined by measuring the electrical conductivity. The results show that the increase in microhardness near the SP surface and the maximum compressive residual stresses are mainly related to the content of alloying elements in the solid solution. Surface roughness increases with increasing SP Almen intensity, and samples with the highest microhardness and residual stresses have the lowest surface roughness.
Mechanochemical Activation and Spark Plasma Sintering of the Lead-Free Ba(Fe1/2Nb1/2)O3 Ceramics
Authors: Bochenek Dariusz , Bartkowska Joanna A. , Kozielski Lucjan , Szafraniak-Wiza Izabela
Published: April 2021

This paper investigates the impact of the technological process (Mechanochemical Activation (MA) of the powder in combination with the Spark Plasma Sintering (SPS) method) on the final properties of lead-free Ba(Fe1/2Nb1/2)O3 (BFN) ceramic materials. The BFN powders were obtained for different MA duration times (x from 10 to 100 h). The mechanically activated BFN powders were used in the technological process of the BFN ceramics by the SPS method. The measurements of the BFNxMA ceramic samples included the following analysis: Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), DC electrical conductivity, and dielectric properties. X-ray diffractions (XRD) tests showed the appearance of the perovskite phase of BFN powders after 10 h of milling time. The longer milling time (up 20 h) causes the amount of the perovskite phase to gradually increase, and the diffraction peaks are more clearly visible. Short high energy milling times favor a large heterogeneity of the grain shape and size. Increasing the MA milling time to 40 h significantly improves the microstructure of BFN ceramics sintered in the SPS technology. The microstructure becomes fine-grained with clearly visible grain boundaries and higher grain size uniformity. Temperature measurements of the BFN ceramics show a number of interesting dielectric properties, i.e., high values of electric permittivity, relaxation properties with a diffusion phase transition, as well as negative values of dielectric properties occurring at high temperatures. The high electric permittivity values predestines the BFNxMA materials for energy storage applications e.g., high energy density batteries, while the negative values of dielectric properties can be used for shield elements against the electromagnetic radiation.
Evaluation of the Cyclic Fatigue of Two Single Files at Body and Room Temperature with Different Radii of Curvature
Authors: La Rosa Giusy Rita Maria , Shumakova Valeria , Isola Gaetano , Indelicato Francesco , Bugea Calogero , Pedullà Eugenio
Published: April 2021

Background: To compare the influence of different temperatures and curvature radii on the cyclic fatigue resistance of F6 SkyTaper (F6ST) and One Curve (OC) single file nickel-titanium rotary instruments. Methods: A total of 120 instruments of F6ST and OC #25.06 were evaluated in 5 mm and 3 mm curvature radii at two temperatures (20 °C ± 1 °C and 37 °C ± 1 °C) in 16 mm stainless steel artificial canals associated with a curvature of 60°. The cyclic fatigue of tested files was assessed by employing a customized testing apparatus and expressed as times to fracture (TtF). A statistical analysis was performed with the significance level set at 95%. Results: All instruments decreased their TtF at 37 °C except for OC in the 3 mm radius, in which no significant difference was detected between 20 °C and 37 °C. A 3 mm curvature radius negatively affected TtF of all tested instruments, except for F6ST at 20 °C. F6ST had higher TtF than OC in the 3 mm radius at 20 °C, with no significant difference between them in the other tested conditions. Conclusions: Under the limits of the present in vitro study, body temperature impaired cyclic fatigue resistance of all files, except for OC in the 3 mm curvature radius. All instruments exhibited lower times to fracture in the 3 mm radius, excluding F6ST at 20 °C.
The Influence of MMA Esterification on Interfacial Adhesion and Mechanical Properties of Hybrid Kenaf Bast/Glass Fiber Reinforced Unsaturated Polyester Composites
Authors: Rahman Rozyanty , Syed Putra Syed Zhafer Firdaus , Abd Rahim Shayfull Zamree , Nainggolan Irwana , Jeż Bartłomiej , Nabiałek Marcin , Musa Luqman , Sandu Andrei Victor , Vizureanu Petrica , Al Bakri Abdullah Mohd Mustafa , Kwiatkowski Dariusz , Wnuk Izabela
Published: April 2021

The demand for natural fiber hybrid composites for various applications has increased, which is leading to more research being conducted on natural fiber hybrid composites due to their promising mechanical properties. However, the incompatibility of natural fiber with polymer matrix limits the performance of the natural fiber hybrid composite. In this research work, the mechanical properties and fiber-to-matrix interfacial adhesion were investigated. The efficiency of methyl methacrylate (MMA)-esterification treatments on composites’ final product performance was determined. The composite was prepared using the hand lay-up method with varying kenaf bast fiber (KBF) contents of 10, 15, 20, 25, 30, 35 (weight%) and hybridized with glass fiber (GF) at 5 and 10 (weight%). Unsaturated polyester (UPE) resin and methyl ethyl ketone peroxide (MEKP) were used as binders and catalysts, respectively. Scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) were used to examine the effects of MMA-esterification treatment on tensile strength and morphology (tensile fracture and characterization of MMA-esterification treatment) of the composite fabricated. The tensile strength of MMA-treated reinforced UPE and hybrid composites are higher than that of untreated composites. As for MMA treatment, 90 min of treatment showed the highest weight percent gain (WPG) and tensile strength of KBF-reinforced UPE composites. It can be concluded that the esterification of MMA on the KBF can lead to better mechanical properties and adhesion between the KFB and the UPE matrix. This research provides a clear reference for developing hybrid natural fibers, thus contributing to the current field of knowledge related to GF composites, specifically in transportation diligences due to their properties of being lightweight, superior, and involving low production cost.
Concurrent Lamination and Tapering Optimization of Cantilever Composite Plates under Shear
Authors: Serhat Gokhan
Published: April 2021

The operational performance of cantilever composite structures can benefit from both stiffness tailoring and geometric design, yet, this potential has not been fully utilized in existing studies. The present study addresses this problem by simultaneously optimizing layer and taper angles of cantilever laminates. The design objective is selected as minimizing the average deflection of the tip edge subjected to shear loads while keeping the length and total volume constant. The plate stiffness properties are described by lamination parameters to eliminate the possible solution dependency on the initial assumptions regarding laminate configuration. The responses are computed via finite element analyses, while optimal design variables are determined using genetic algorithms. The results demonstrate that the plate aspect ratio significantly influences the effectiveness of stiffness tailoring and tapering as well as the optimal layer and taper angles. In addition, concurrent exploitation of the lamination characteristics and plate geometry is shown to be essential for achieving maximum performance. Moreover, individual and simultaneous optimization of layer and taper angles produce different optimal results, indicating the possible drawback of using sequential approaches in similar composite design problems.
Clinching of Thermoplastic Composites and Metals—A Comparison of Three Novel Joining Technologies
Authors: Gröger Benjamin , Troschitz Juliane , Vorderbrüggen Julian , Vogel Christian , Kupfer Robert , Meschut Gerson , Gude Maik
Published: April 2021

Clinching continuous fibre reinforced thermoplastic composites and metals is challenging due to the low ductility of the composite material. Therefore, a number of novel clinching technologies has been developed specifically for these material combinations. A systematic overview of these advanced clinching methods is given in the present paper. With a focus on process design, three selected clinching methods suitable for different joining tasks are described in detail. The clinching processes including equipment and tools, observed process phenomena and the resultant material structure are compared. Process phenomena during joining are explained in general and compared using computed tomography and micrograph images for each process. In addition the load bearing behaviour and the corresponding failure mechanisms are investigated by means of single-lap shear tests. Finally, the new joining technologies are discussed regarding application relevant criteria.
ANN-Based Pattern Recognition for Induction Motor Broken Rotor Bar Monitoring under Supply Frequency Regulation
Authors: Sinha Ashish Kumar , Hati Ananda Shankar , Benbouzid Mohamed , Chakrabarti Prasun
Published: April 2021

The requisite of direct-on-line (DOL) starting for various applications in underground mines subjects the rotor bars of heavy-duty squirrel cage induction motors (SCIMs) to severe stresses, resulting in sustained fault in the rotor bars, unlike the applications where mostly reduced voltage starting is preferred. Furthermore, SCIMs working in underground mines are also affected by unforeseen frequency fluctuations. Hence, the paper proposes a discrete wavelet transform (DWT)-based broken rotor bar detection scheme using the stator current analysis of SCIM when subjected to a frequency regulation (±4% of 50 Hz supply) in steady-state, as prevalent in underground mines. In this regard, the level-seven detailed coefficient obtained by the DWT-based multi-resolution analysis of stator current corresponding to the healthy rotor is compared with that of the faulty rotor to extract the necessary features to identify the fault. Further implementation of the proposed scheme is done using artificial neural network (ANN)-based pattern recognition techniques, wherein both feed-forward backdrops and cascaded forward backdrop type ANNs have been used for fault pinpointing based on the feature extraction results obtained from DWT. The scheme is developed and analysed in MATLAB/Simulink using 5.5 kW, 415 V, 50 Hz SCIM, which is further validated using the LabVIEW-based real-time implementation.
Analyzing Medical Data by Using Statistical Learning Models
Authors: Mariani Maria C. , Biney Francis , Tweneboah Osei K.
Published: April 2021

In this work, we investigated the prognosis of three medical data specifically, breast cancer, heart disease, and prostate cancer by using 10 machine learning models. We applied all 10 models to each dataset to identify patterns in them. Furthermore, we use the models to diagnose risk factors that increases the chance of these diseases. All the statistical learning techniques discussed were grouped into linear and nonlinear models based on their similarities and learning styles. The models performances were significantly improved by selecting models while taking into account the bias-variance tradeoffs and using cross-validation for selecting the tuning parameter. Our results suggests that no particular class of models or learning style dominated the prognosis and diagnosis for all three medical datasets. However nonlinear models gave the best predictive performance for breast cancer data. Linear models on the other hand gave the best predictive performance for heart disease data and a combination of linear and nonlinear models for the prostate cancer dataset.
Evaluation of Human Resources in Transportation Companies Using Multi-Criteria Model for Ranking Alternatives by Defining Relations between Ideal and Anti-Ideal Alternative (RADERIA)
Authors: Jakovljevic Vladimir , Zizovic Mališa , Pamucar Dragan , Stević Željko , Albijanic Miloljub
Published: April 2021

Multi-criteria decision-making methods (MCDM) represent a very powerful tool for making decisions in different areas. Making a rational and reliable decision, while respecting different factors, is a challenging and difficult task; MCDM models have a great impact on achieving this goal. In this paper, a new MCDM technique is presented—ranking alternatives by defining relations between the ideal and anti-ideal alternative (RADERIA), which was tested for the evaluation of human resources (HR) in a transportation company. The RADERIA model has three key advantages that recommend it for future use: (1) the RADERIA model has a new approach for data normalization that enables defining the normalization interval according to the judgments of a decision-maker; (2) an adaptive model for data normalization of the RADERIA model allows tough conversion into various forms of decreasing functions (linear, quadratic equation, etc.); and (3) the resistance of the RADERIA model to the rank reversal problem. Furthermore, in many simulations, the RADERIA method has shown stability when processing a larger number of datasets. This was also confirmed by a case study with 36 alternatives, as considered in this paper. The results and verification of the proposed new method were acquired through a comprehensive verification of the complexity of the results. The complexity of the results was executed through (1) comparison with four other multi-criteria methods, (2) checking the resistance of the RADERIA model to the rank reversal problem, and (3) the analysis of the impact of changes in the measurement scale on the ranking results.
Environmental Impact on Seaweed Phenolic Production and Activity: An Important Step for Compound Exploitation
Authors: Lomartire Silvia , Cotas João , Pacheco Diana , Marques João Carlos , Pereira Leonel , Gonçalves Ana M. M.
Published: April 2021

Seaweeds are a potential source of bioactive compounds that are useful for biotechnological applications and can be employed in different industrial areas in order to replace synthetic compounds with components of natural origin. Diverse studies demonstrate that there is a solid ground for the exploitation of seaweed bioactive compounds in order to prevent illness and to ensure a better and healthier lifestyle. Among the bioactive algal molecules, phenolic compounds are produced as secondary metabolites with beneficial effects on plants, and also on human beings and animals, due to their inherent bioactive properties, which exert antioxidant, antiviral, and antimicrobial activities. The use of phenolic compounds in pharmaceutical, nutraceutical, cosmetics, and food industries may provide outcomes that could enhance human health. Through the production of healthy foods and natural drugs, bioactive compounds from seaweeds can help with the treatment of human diseases. This review aims to highlight the importance of phenolic compounds from seaweeds, the scope of their production in nature and the impact that these compounds can have on human and animal health through nutraceutical and pharmaceutical products.
Heritability of Cardiothoracic Ratio and Aortic Arch Calcification in Twins
Authors: Jokkel Zsofia , Forgo Bianka , Hani-Gaius Ghattas Christopher , Piroska Marton , Szabó Helga , Tarnoki David L. , Tarnoki Adam D. , Lee Sooji , Sung Joohon
Published: April 2021

Background and Objectives: Aortic arch calcification (AoAC) is associated with a variety of cardiovascular complications. The measurement and grading of AoAC using posteroanterior (PA) chest X-rays are well established. The cardiothoracic ratio (CTR) can be simultaneously measured with PA chest X-rays and used as an index of cardiomegaly. The genetic and environmental contributions to the degree of the AoAC and CTR are not well understood. The purpose of this study was to investigate the effect of genetics and environmental factors on the AoAC and CTR. Materials and Methods: A total of 684 twins from the South Korean twin registry (261 monozygotic, MZ and 81 dizygotic, DZ pairs; mean age 38.6 ± 7.9 years, male/female = 264/420) underwent PA chest X-rays. Cardiovascular risk factors and anthropometric data were also collected. The AoAC and CTR were measured and graded using a standardized method. A structural equation method was used to calculate the proportion of variance explained by genetic and environmental factors behind AoAC and CTR. Results: The within-pair differences were low regarding the grade of AoAC, with only a few twin pairs showing large intra-pair differences. We found that the thoracic width showed high heritability (0.67, 95% CI: 0.59–0.73, p = 1). Moderate heritability was detected regarding cardiac width (0.54, 95% CI: 0.45–0.62, p = 0.572) and CTR (0.54, 95% CI: 0.44–0.62, p = 0.701). Conclusions: The heritable component was significant regarding thoracic width, cardiac width, and the CTR.
Effect of Silica Sodalite Functionalization and PVA Coating on Performance of Sodalite Infused PSF Membrane during Treatment of Acid Mine Drainage
Authors: Ntshangase Nobuhle C. , Sadare Olawumi O. , Daramola Michael O.
Published: April 2021

In this study, silica sodalite (SSOD) nanoparticles were synthesized by topotactic conversion and functionalized using HNO3/H2SO4 (1:3). The SSOD and functionalized SSOD (fSSOD) nanoparticles were infused into a Polysulfone (Psf) membrane to produce mixed matrix membranes. The membranes were fabricated via the phase inversion method. The membranes and the nanoparticles were characterized using Scanning Electron Microscopy (SEM) to check the morphology of the nanoparticles and the membranes and Fourier Transform Infrared to check the surface chemistry of the nanoparticles and the membranes. Thermal stability of the nanoparticles and the membranes was evaluated using Themogravimetry analysis (TGA) and the degree of hydrophilicity of the membranes was checked via contact angle measurements. The mechanical strength of the membranes and their surface nature (roughness) were checked using a nanotensile instrument and Atomic Force Microscopy (AFM), respectively. The textural property of the nanoparticles were checked by conducting N2 physisorption experiments on the nanoparticles at 77 K. AMD-treatment performance of the fabricated membranes was evaluated in a dead-end filtration cell using a synthetic acid mine drainage (AMD) solution prepared by dissolving a known amount of MgCl2, MnCl2·4H2O, Na2SO4, Al(NO3)3, Fe(NO3)3·9H2O, and Ca2OH2 in deionized water. Results from the N2 physisorption experiments on the nanoparticles at 77 K showed a reduction in surface area and increase in pore diameter of the nanoparticles after functionalization. Performance of the membranes during AMD treatment shows that, at 4 bar, a 10% fSSOD/Psf membrane displayed improved heavy metal rejection >50% for all heavy metals considered, expect the SSOD-loaded membrane that showed a rejection <13% (except for Al3+ 89%). In addition, coating the membranes with a PVA layer improved the antifouling property of the membranes. The effects of multiple PVA coating and behaviour of the membranes during real AMD are not reported in this study, these should be investigated in a future study. Therefore, the newly developed functionalized SSOD infused Psf membranes could find applications in the treatment of AMD or for the removal of heavy metals from wastewater.
Lateral Degassing Method for Disposable Film-Chip Microfluidic Devices
Authors: Park Suhee , Cho Hyungseok , Kim Junhyeong , Han Ki-Ho
Published: April 2021

It is critical to develop a fast and simple method to remove air bubbles inside microchannels for automated, reliable, and reproducible microfluidic devices. As an active degassing method, this study introduces a lateral degassing method that can be easily implemented in disposable film-chip microfluidic devices. This method uses a disposable film-chip microchannel superstrate and a reusable substrate, which can be assembled and disassembled simply by vacuum pressure. The disposable microchannel superstrate is readily fabricated by bonding a microstructured polydimethylsiloxane replica and a silicone-coated release polymeric thin film. The reusable substrate can be a plate that has no function or is equipped with the ability to actively manipulate and sense substances in the microchannel by an elaborately patterned energy field. The degassing rate of the lateral degassing method and the maximum available pressure in the microchannel equipped with lateral degassing were evaluated. The usefulness of this method was demonstrated using complex structured microfluidic devices, such as a meandering microchannel, a microvortex, a gradient micromixer, and a herringbone micromixer, which often suffer from bubble formation. In conclusion, as an easy-to-implement and easy-to-use technique, the lateral degassing method will be a key technique to address the bubble formation problem of microfluidic devices.
Block Copolymer Template-Directed Catalytic Systems: Recent Progress and Perspectives
Authors: Kumar Labeesh , Singh Sajan , Horechyy Andriy , Fery Andreas , Nandan Bhanu
Published: April 2021

Fabrication of block copolymer (BCP) template-assisted nano-catalysts has been a subject of immense interest in the field of catalysis and polymer chemistry for more than two decades now. Different methods, such as colloidal route, on-substrate methods, bulk self-assembly approaches, combined approaches, and many others have been used to prepare such nano-catalysts. The present review focuses on the advances made in this direction using diblock, triblock, and other types of BCP self-assembled structures. It will be shown how interestingly, researchers have exploited the features of tunable periodicity, domain orientation, and degree of lateral orders of self-assembled BCPs by using fundamental approaches, as well as using different combinations of simple methods to fabricate efficient catalysts. These approaches allow for fabricating catalysts that are used for the growth of single- and multi-walled carbon nanotubes (CNTs) on the substrate, size-dependent electrooxidation of the carbon mono oxide, cracking of 1,3,5-triisopropylbenzene (TIPB), methanol oxidation, formic acid oxidation, and for catalytic degradation of dyes and water pollutants, etc. The focus will also be on how efficient and ease-of-use catalysts can be fabricated using different BCP templates, and how these have contributed to the fabrication of different nano-catalysts, such as nanoparticle array catalysts, strawberry and Janus-like nanoparticles catalysts, mesoporous nanoparticles and film catalysts, gyroid-based bicontinuous catalysts, and hollow fiber membrane catalysts.
Thermokinetic Modelling of High-Temperature Evolution of Primary Nb(C,N) in Austenite Applied to Recrystallization of 316Nb Austenitic Stainless Steel
Authors: Cliche Nicolas , Ringeval Sylvain , Petit Philippe , Bellus Jacques , Georges Eric , Cortial François , Heuzé Jean-Loup , Gourgues-Lorenzon Anne-Françoise , Esin Vladimir A.
Published: April 2021

The size evolution of niobium carbonitrides Nb(C,N) and the evolution of the composition of an austenitic matrix in 316Nb stainless steel were simulated using DICTRA software. For the first time, the complete nine-element composition of steel was taken into account during isothermal and even anisothermal heat treatments. A reduced model was then proposed to optimize the calculation time for complex heat treatments. The change in the mean Nb content in austenite due to Nb(C,N) evolution during different heat treatments was studied. It qualitatively agrees with experimental data as obtained by electron probe microanalysis. Furthermore, the model was successfully applied to explain the effect of heat treatments on the recrystallization behavior of 316Nb steel during hot torsion tests. Moreover, the effect of the thermodynamic database and the number of alloying elements chosen was discussed. We showed that taking into account seven or even nine elements greatly improves the accuracy compared to usual simplified compositions. The proposed method can be useful in designing heat treatments promoting or conversely hindering recrystallization for a wide variety of Nb-bearing steels.
Reduction of Residual Quenching Stresses in 2A14 Aluminum Alloy Tapered Cylinder Forgings via a Novel Cold Bulging Process
Authors: Wang Bingxiang , Yi Youping , Huang Shiquan , He Hailin
Published: April 2021

This study combined finite element method (FEM) simulations and physical experiments to develop a novel cold bulging process, with the aim of studying and mitigating the quenching residual stresses in 2A14 tapered cylinder forgings. The samples underwent cold bulging at different ratios (0–4.0%) to evaluate the residual stress reduction performance (via the hole-drilling strain-gauge method) and the improvements in their mechanical properties. The FEM simulation and experimental results revealed that our proposed cold bulging process reduced the quenching residual stresses by up to 85–87%. The density and uniformity of the precipitated phases increased along with the extent of cold bulging, as confirmed by transmission electron microscope (TEM) observations. Furthermore, compared to the unprocessed samples, the tensile and yield strengths, and elongation of the samples with 3% cold bulging were significantly enhanced (65 MPa, 55 MPa, and 1.7%, respectively).
Hepatitis C Virus Uses Host Lipids to Its Own Advantage
Authors: Sidorkiewicz Malgorzata
Published: April 2021

Lipids and lipoproteins constitute indispensable components for living not only for humans. In the case of hepatitis C virus (HCV), the option of using the products of our lipid metabolism is “to be, or not to be”. On the other hand, HCV infection, which is the main cause of chronic hepatitis, cirrhosis and hepatocellular carcinoma, exerts a profound influence on lipid and lipoprotein metabolism of the host. The consequences of this alternation are frequently observed as hypolipidemia and hepatic steatosis in chronic hepatitis C (CHC) patients. The clinical relevance of these changes reflects the fact that lipids and lipoprotein play a crucial role in all steps of the life cycle of HCV. The virus circulates in the bloodstream as a highly lipidated lipo-viral particle (LVP) that defines HCV hepatotropism. Thus, strict relationships between lipids/lipoproteins and HCV are indispensable for the mechanism of viral entry into hepatocytes, viral replication, viral particles assembly and secretion. The purpose of this review is to summarize the tricks thanks to which HCV utilizes host lipid metabolism to its own advantage.