bluuebunny/crossref_metadata_2025 huggingface dataset:
Spatiotemporal Distribution and Influencing Factors of Ecosystem Vulnerability on Qinghai-Tibet Plateau
Authors: Li Han , Song Wei
Published: June 2021

As the “Third Pole”, the Qinghai-Tibet Plateau is threatened by environmental changes. Ecosystem vulnerability refers to the sensitivity and resilience of ecosystems to external disturbances. However, there is a lack of relevant studies on the driving factors of ecosystem vulnerability. Therefore, based on spatial principal components analysis and geographic detectors methods, this paper evaluates the ecosystem vulnerability and its driving factors on the Qinghai-Tibet Plateau from the years 2005 to 2015. The results were as follows: (1) The ecosystem vulnerability index (EVI) of the Qinghai-Tibet Plateau is mainly heavy and extreme, showing a gradually increasing trend from southeast to northwest. (2) The spatial heterogeneity of the EVI is significant in the southeast and northwest, but not in the southwest and central parts. (3) Analysis of influencing factors shows that environmental factors have more significant effects on EVI than socioeconomic variables, facilitating the proposal of adequate policy implications. More efforts should be devoted to ecological protection and restoration to prevent grassland degradation and desertification in the high-EVI areas in northwest. The government is also urged to improve the ecological compensation mechanisms and balance ecological protection and residents’ development needs in the southeast.
Impact of COVID-19 on Healthcare Workers in Brazil between August and November 2020: A Cross-Sectional Survey
Authors: Faria de Moura Villela Edlaine , Rodrigues da Cunha Izadora , Nelson Siewe Fodjo Joseph , Obimpeh Michael , Colebunders Robert , Van Hees Stijn
Published: June 2021

During the COVID-19 pandemic, healthcare workers (HCW) have been subjected to greater workloads. We conducted a cross-sectional online survey to assess the impact of the COVID-19 pandemic on Brazilian HCW. Data were collected between 11 August and 1 November 2020. Of the 295 respondents, 95 (32.2%) were medical doctors, 82 (27.8%) administrative staff, 53 (18.0%) nurses, 27 (9.2%) laboratory staff, and 38 (12.9%) were other staff. COVID-19-related restructuring at the health facilities was reported by 207 (70.2%) respondents, and 69 (23.4%) had their tasks changed. Preventive measures were well respected when seeing suspected patients. Overall, 167 (56.6%) HCW screened positive for anxiety and 137 (46.4%) for depression; 109 (36.9%) screened positive for both conditions. Of the 217 (73.6%) HCW who had been tested for COVID-19, at least one positive result was reported in 49 (22.6%). Following a positive COVID-19 test, 45/49 (91.8%) stopped working and stayed home. In conclusion, we found a high incidence of COVID-19 infection among Brazilian HCW with high rates of anxiety and depression despite a good self-reported adherence to COVID-19 preventive measures. As such, our study highlights the urgent need for interventions to mitigate the psychosocial risks HCW in Brazil encounter during the COVID-19 pandemic.
Neuromuscular Fitness Is Associated with Success in Sport for Elite Female, but Not Male Tennis Players
Authors: Dobos Karoly , Novak Dario , Barbaros Petar
Published: June 2021

Background: The purpose of the study was to examine whether neuromuscular fitness contributes significantly to the success of eAlite junior tennis players of differing ages and sexes. Methods: The 160 participants, who were elite Hungarian junior tennis players (aged 11–17), were separated into four groups within this study, and 10 different types of field tests were used. Results: A moderate significant correlation was found between the results of the 5 m run (r = −0.42; r = −0.45), standing long jump (r = 0.39; r = 0.56), overhand ball throw (r = 0.44; r = 0.53), serve (r = 0.39; r = 0.64), amount of push-ups in 30 seconds (r = 0.32; r = 0.48), 10 × 5 m run in a shuttle run (r = −0.34; r = −0.45), the spider run (r = −0.34; r = −0.52), and competitive tennis success among U14 and U18 girls. A significant correlation between the overhead medicine ball throw test value (r = 0.47) and the current competitive performance was found only among U18 elite female tennis players. In contrast, no correlation was found between the values of the U14 and U18 male tennis players and their current competitive performance. Conclusions: Additional studies are needed to identify interventions that can increase sport-specific neuromuscular fitness with the ultimate goal of achieving better performance.
Edible Insects: How to Increase the Sustainable Consumption Behavior among Restaurant Consumers
Authors: Hwang Jinsoo , Kim Jinkyung-Jenny
Published: June 2021

Edible insects deserve increased attention as green food source in today’s society and more restaurants embrace them to promote sustainable consumption behavior. This study was design to explore how consumers’ behavioral intentions to use edible insect restaurants were formed based on the theory of planned behavior (TPB) model. Furthermore, the study attempted to deepen TPB by including the moderating role of product knowledge. A total of 440 samples were collected by online survey in South Korea, and the results of structural equation modeling found that all of the hypotheses have been statistically accepted. Additionally, the results of multiple group analysis indicated that product knowledge moderated the link between subjective norm and behavioral intentions. On a basis of the analysis results, we provided significant theoretical implications and practical implications how to increase future sustainable food consumption intention.
Social Support for People with Morbid Obesity in a Bariatric Surgery Programme: A Qualitative Descriptive Study
Authors: Torrente-Sánchez María José , Ferrer-Márquez Manuel , Estébanez-Ferrero Beatriz , Jiménez-Lasserrotte María del Mar , Ruiz-Muelle Alicia , Ventura-Miranda María Isabel , Dobarrio-Sanz Iria , Granero-Molina José
Published: June 2021

Background—Morbid obesity (MO) is a chronic metabolic disease affecting physical, psychological and social wellbeing. Bariatric surgery is a reliable method for losing weight in the long term, improving the quality of life, body image and social life of people with MO. Current literature recognises the importance of social support in controlling weight and coping with MO. The objective of this study was to describe and understand experiences related to social support for patients with MO included in a bariatric surgery programme. Methods—A qualitative descriptive study, where data collection included thirty-one interviews with people diagnosed with MO involved in a bariatric surgery programme. Results—Three main themes emerged from the analysis: (1) accepting the problem in order to ask for help, (2) the need for close support and (3) professional support: opposing feelings. Conclusions—A partner, family and friends are the key pillars of social support for those with MO included in a bariatric surgery programme. Healthcare professionals gave formal support; the bariatric surgery team provided information, trust and assurance. Nurses provided healthcare 24 h a day, making them the main formal support for people in the bariatric surgery programme.
Mental Health Status and Its Influencing Factors: The Case of Nurses Working in COVID-19 Hospitals in South Korea
Authors: Kim Min-Young , Yang Yun-Yi
Published: June 2021

The mental health of nurses participating in patient care is under threat amid the coronavirus disease 2019 (COVID-19) pandemic. This study aimed to identify the mental health status (depression, anxiety, and stress) and its influencing factors on nurses who provided patient care at a specialized hospital for COVID-19 in South Korea. Of the 180 nurses who participated in this study, 30.6% had moderate or higher levels of depression, 41% had moderate or higher anxiety levels, and 19.4% had moderate or higher stress levels. In this study, stigma influenced nurses’ mental health, such that the higher the stigma, the higher the nurses’ depression, anxiety, and stress. Depression was higher in female nurses than in male nurses, and stress was higher in charge nurses than nurses in other job positions. Therefore, a management program should be designed to improve the mental health of nurses during the current pandemic. In particular, a solution to reduce stigma is required, and the mental health of female nurses and nurses in leadership roles requires special attention.
Precision Health Care Elements, Definitions, and Strategies for Patients with Diabetes: A Literature Review
Authors: Pranata Satriya , Wu Shu-Fang Vivienne , Alizargar Javad , Liu Ju-Han , Liang Shu-Yuan , Lu Yu-Ying
Published: June 2021

Diabetes is a prevalent disease with a high risk of complications. The number of people with diabetes worldwide was reported to increase every year. However, new integrated individualized health care related to diabetes is insufficiently developed. Purpose: The objective of this study was to conduct a literature review and discover precision health care elements, definitions, and strategies. Methods: This study involved a 2-stage process. The first stage comprised a systematic literature search, evidence evaluation, and article extraction. The second stage involved discovering precision health care elements and defining and developing strategies for the management of patients with diabetes. Results: Of 1337 articles, we selected 35 relevant articles for identifying elements and definitions of precision health care for diabetes, including personalized genetic or lifestyle factors, biodata- or evidence-based practice, glycemic target, patient preferences, glycemic control, interdisciplinary collaboration practice, self-management, and patient priority direct care. Moreover, strategies were developed to apply precision health care for diabetes treatment based on eight elements. Conclusions: We discovered precision health care elements and defined and developed strategies of precision health care for patients with diabetes. precision health care is based on team foundation, personalized glycemic target, and control as well as patient preferences and priority, thus providing references for future research and clinical practice.
Incidence and Characteristics of Retinoblastoma in Poland: The First Nationwide Study 2010–2017
Authors: Nowak Michał S. , Romanowska-Dixon Bożena , Grabska-Liberek Iwona , Żurek Michał
Published: June 2021

Background: The present study aimed to investigate the incidence and characteristics of retinoblastoma in the overall population of Poland. Methods: The retrospective survey of both National Health Fund (NHF) and National Cancer Registry (NCR) databases were performed to identify all retinoblastoma cases in Poland in the years 2010–2017. Results: During 2010–2017, the mean age-standardised incidence of retinoblastoma (the unit of incidence is per 1,000,000 person-years) was 10.15 (95% CI 7.23–13.08) among children aged 0 to 4 years and 5.39 (95% CI 4.18–6.60) in those aged 0 to 9 years. During 2010–2014 (to allow 5 years of follow-up), the mean incidence of retinoblastoma by birth cohort analysis in Poland was 4.89 (95% CI 4.04–5.74) per 100,000 live births, corresponding to an incidence of 1 per 20,561 (95% CI 15,855–25,267) live births. In Poland, 14.6% of children with retinoblastoma had enucleation of the eye globe, 76.8% received different types of chemotherapy combined with focal treatment, 5.9% were treated with external beam radiotherapy, and 2.7% were treated with focal treatments only. Conclusions: The incidence of retinoblastoma and the pattern of medical management of retinoblastoma in Poland was similar to that reported in developed countries in Western Europe, Asia, and North America.
Long-Chain Acylcarnitines Decrease the Phosphorylation of the Insulin Receptor at Tyr1151 Through a PTP1B-Dependent Mechanism
Authors: Vilks Karlis , Videja Melita , Makrecka-Kuka Marina , Katkevics Martins , Sevostjanovs Eduards , Grandane Aiga , Dambrova Maija , Liepinsh Edgars
Published: June 2021

The accumulation of lipid intermediates may interfere with energy metabolism pathways and regulate cellular energy supplies. As increased levels of long-chain acylcarnitines have been linked to insulin resistance, we investigated the effects of long-chain acylcarnitines on key components of the insulin signalling pathway. We discovered that palmitoylcarnitine induces dephosphorylation of the insulin receptor (InsR) through increased activity of protein tyrosine phosphatase 1B (PTP1B). Palmitoylcarnitine suppresses protein kinase B (Akt) phosphorylation at Ser473, and this effect is not alleviated by the inhibition of PTP1B by the insulin sensitizer bis-(maltolato)-oxovanadium (IV). This result indicates that palmitoylcarnitine affects Akt activity independently of the InsR phosphorylation level. Inhibition of protein kinase C and protein phosphatase 2A does not affect the palmitoylcarnitine-mediated inhibition of Akt Ser473 phosphorylation. Additionally, palmitoylcarnitine markedly stimulates insulin release by suppressing Akt Ser473 phosphorylation in insulin-secreting RIN5F cells. In conclusion, long-chain acylcarnitines activate PTP1B and decrease InsR Tyr1151 phosphorylation and Akt Ser473 phosphorylation, thus limiting the cellular response to insulin stimulation.
Antiviral Activity of Two Marine Carotenoids against SARS-CoV-2 Virus Entry In Silico and In Vitro
Authors: Yim Sung-Kun , Kim Inhee , Warren Boyd , Kim Jungwon , Jung Kyoojin , Ku Bosung
Published: June 2021

The marine carotenoids fucoxanthin and siphonaxanthin are powerful antioxidants that are attracting focused attention to identify a variety of health benefits and industry applications. In this study, the binding energy of these carotenoids with the SARS-CoV-2 Spike-glycoprotein was predicted by molecular docking simulation, and their inhibitory activity was confirmed with SARS-CoV-2 pseudovirus on HEK293 cells overexpressing angiotensin-converting enzyme 2 (ACE2). Siphonaxanthin from Codium fragile showed significant antiviral activity with an IC50 of 87.4 μM against SARS-CoV-2 pseudovirus entry, while fucoxanthin from Undaria pinnatifida sporophyll did not. The acute toxicities were predicted to be relatively low, and pharmacokinetic predictions indicate GI absorption. Although further studies are needed to elucidate the inhibition of viral infection by siphonaxanthin, these results provide useful information in the application of these marine carotenoids for the treatment and prevention of COVID-19.
Soluble Cytoplasmic Expression and Purification of Immunotoxin HER2(scFv)-PE24B as a Maltose Binding Protein Fusion
Authors: Park Sangsu , Nguyen Minh Quan , Ta Huynh Kim Khanh , Nguyen Minh Tan , Lee Gunsup , Kim Chong Jai , Jang Yeon Jin , Choe Han
Published: June 2021

Human epidermal growth factor receptor 2 (HER-2) is overexpressed in many malignant tumors. The anti-HER2 antibody trastuzumab has been approved for treating HER2-positive early and metastatic breast cancers. Pseudomonas exotoxin A (PE), a bacterial toxin of Pseudomonas aeruginosa, consists of an A-domain with enzymatic activity and a B-domain with cell binding activity. Recombinant immunotoxins comprising the HER2(scFv) single-chain Fv from trastuzumab and the PE24B catalytic fragment of PE display promising cytotoxic effects, but immunotoxins are typically insoluble when expressed in the cytoplasm of Escherichia coli, and thus they require solubilization and refolding. Herein, a recombinant immunotoxin gene was fused with maltose binding protein (MBP) and overexpressed in a soluble form in E. coli. Removal of the MBP yielded stable HER2(scFv)-PE24B at 91% purity; 0.25 mg of pure HER2(scFv)-PE24B was obtained from a 500 mL flask culture. Purified HER2(scFv)-PE24B was tested against four breast cancer cell lines differing in their surface HER2 level. The immunotoxin showed stronger cytotoxicity than HER2(scFv) or PE24B alone. The IC50 values for HER2(scFv)-PE24B were 28.1 ± 2.5 pM (n = 9) and 19 ± 1.4 pM (n = 9) for high HER2-positive cell lines SKBR3 and BT-474, respectively, but its cytotoxicity was lower against MDA-MB-231 and MCF7. Thus, fusion with MBP can facilitate the soluble expression and purification of scFv immunotoxins.
Engineering of Janus-Like Dendrimers with Peptides Derived from Glycoproteins of Herpes Simplex Virus Type 1: Toward a Versatile and Novel Antiviral Platform
Authors: Falanga Annarita , Del Genio Valentina , Kaufman Elizabeth A. , Zannella Carla , Franci Gianluigi , Weck Marcus , Galdiero Stefania
Published: June 2021

Novel antiviral nanotherapeutics, which may inactivate the virus and block it from entering host cells, represent an important challenge to face viral global health emergencies around the world. Using a combination of bioorthogonal copper-catalyzed 1,3-dipolar alkyne/azide cycloaddition (CuAAC) and photoinitiated thiol–ene coupling, monofunctional and bifunctional peptidodendrimer conjugates were obtained. The conjugates are biocompatible and demonstrate no toxicity to cells at biologically relevant concentrations. Furthermore, the orthogonal addition of multiple copies of two different antiviral peptides on the surface of a single dendrimer allowed the resulting bioconjugates to inhibit Herpes simplex virus type 1 at both the early and the late stages of the infection process. The presented work builds on further improving this attractive design to obtain a new class of therapeutics.
Roles of Nuclear Receptors in Vascular Calcification
Authors: Chinetti Giulia , Neels Jaap G.
Published: June 2021

Vascular calcification is defined as an inappropriate accumulation of calcium depots occurring in soft tissues, including the vascular wall. Growing evidence suggests that vascular calcification is an actively regulated process, sharing similar mechanisms with bone formation, implicating both inhibitory and inducible factors, mediated by osteoclast-like and osteoblast-like cells, respectively. This process, which occurs in nearly all the arterial beds and in both the medial and intimal layers, mainly involves vascular smooth muscle cells. In the vascular wall, calcification can have different clinical consequences, depending on the pattern, localization and nature of calcium deposition. Nuclear receptors are transcription factors widely expressed, activated by specific ligands that control the expression of target genes involved in a multitude of pathophysiological processes, including metabolism, cancer, inflammation and cell differentiation. Some of them act as drug targets. In this review we describe and discuss the role of different nuclear receptors in the control of vascular calcification.
Hepcidin-Ferroportin Interaction Controls Systemic Iron Homeostasis
Authors: Nemeth Elizabeta , Ganz Tomas
Published: June 2021

Despite its abundance in the environment, iron is poorly bioavailable and subject to strict conservation and internal recycling by most organisms. In vertebrates, the stability of iron concentration in plasma and extracellular fluid, and the total body iron content are maintained by the interaction of the iron-regulatory peptide hormone hepcidin with its receptor and cellular iron exporter ferroportin (SLC40a1). Ferroportin exports iron from duodenal enterocytes that absorb dietary iron, from iron-recycling macrophages in the spleen and the liver, and from iron-storing hepatocytes. Hepcidin blocks iron export through ferroportin, causing hypoferremia. During iron deficiency or after hemorrhage, hepcidin decreases to allow iron delivery to plasma through ferroportin, thus promoting compensatory erythropoiesis. As a host defense mediator, hepcidin increases in response to infection and inflammation, blocking iron delivery through ferroportin to blood plasma, thus limiting iron availability to invading microbes. Genetic diseases that decrease hepcidin synthesis or disrupt hepcidin binding to ferroportin cause the iron overload disorder hereditary hemochromatosis. The opposite phenotype, iron restriction or iron deficiency, can result from genetic or inflammatory overproduction of hepcidin.
Cu and Cu-SWCNT Nanoparticles’ Suspension in Pulsatile Casson Fluid Flow via Darcy–Forchheimer Porous Channel with Compliant Walls: A Prospective Model for Blood Flow in Stenosed Arteries
Authors: Ali Amjad , Bukhari Zainab , Umar Muhammad , Ismail Muhammad Ali , Abbas Zaheer
Published: June 2021

The use of experimental relations to approximate the efficient thermophysical properties of a nanofluid (NF) with Cu nanoparticles (NPs) and hybrid nanofluid (HNF) with Cu-SWCNT NPs and subsequently model the two-dimensional pulsatile Casson fluid flow under the impact of the magnetic field and thermal radiation is a novelty of the current study. Heat and mass transfer analysis of the pulsatile flow of non-Newtonian Casson HNF via a Darcy–Forchheimer porous channel with compliant walls is presented. Such a problem offers a prospective model to study the blood flow via stenosed arteries. A finite-difference flow solver is used to numerically solve the system obtained using the vorticity stream function formulation on the time-dependent governing equations. The behavior of Cu-based NF and Cu-SWCNT-based HNF on the wall shear stress (WSS), velocity, temperature, and concentration profiles are analyzed graphically. The influence of the Casson parameter, radiation parameter, Hartmann number, Darcy number, Soret number, Reynolds number, Strouhal number, and Peclet number on the flow profiles are analyzed. Furthermore, the influence of the flow parameters on the non-dimensional numbers such as the skin friction coefficient, Nusselt number, and Sherwood number is also discussed. These quantities escalate as the Reynolds number is enhanced and reduce by escalating the porosity parameter. The Peclet number shows a high impact on the microorganism’s density in a blood NF. The HNF has been shown to have superior thermal properties to the traditional one. These results could help in devising hydraulic treatments for blood flow in highly stenosed arteries, biomechanical system design, and industrial plants in which flow pulsation is essential.
Fluorescence Liquid Biopsy for Cancer Detection Is Improved by Using Cationic Dendronized Hyperbranched Polymer
Authors: Morcuende-Ventura Violeta , Hermoso-Durán Sonia , Abian-Franco Natalia , Pazo-Cid Roberto , Ojeda Jorge L. , Vega Sonia , Sanchez-Gracia Oscar , Velazquez-Campoy Adrian , Sierra Teresa , Abian Olga
Published: June 2021

(1) Background: Biophysical techniques applied to serum samples characterization could promote the development of new diagnostic tools. Fluorescence spectroscopy has been previously applied to biological samples from cancer patients and differences from healthy individuals were observed. Dendronized hyperbranched polymers (DHP) based on bis(hydroxymethyl)propionic acid (bis-MPA) were developed in our group and their potential biomedical applications explored. (2) Methods: A total of 94 serum samples from diagnosed cancer patients and healthy individuals were studied (20 pancreatic ductal adenocarcinoma, 25 blood donor, 24 ovarian cancer, and 25 benign ovarian cyst samples). (3) Results: Fluorescence spectra of serum samples (fluorescence liquid biopsy, FLB) in the presence and the absence of DHP-bMPA were recorded and two parameters from the signal curves obtained. A secondary parameter, the fluorescence spectrum score (FSscore), was calculated, and the diagnostic model assessed. For pancreatic ductal adenocarcinoma (PDAC) and ovarian cancer, the classification performance was improved when including DHP-bMPA, achieving high values of statistical sensitivity and specificity (over 85% for both pathologies). (4) Conclusions: We have applied FLB as a quick, simple, and minimally invasive promising technique in cancer diagnosis. The classification performance of the diagnostic method was further improved by using DHP-bMPA, which interacted differentially with serum samples from healthy and diseased subjects. These preliminary results set the basis for a larger study and move FLB closer to its clinical application, providing useful information for the oncologist during patient diagnosis.
An Overview of the Role of Long Non-Coding RNAs in Human Choriocarcinoma
Authors: Di Fiore Riccardo , Suleiman Sherif , Felix Ana , O’Toole Sharon A. , O’Leary John J. , Ward Mark P. , Beirne James , Sabol Maja , Ozretić Petar , Yordanov Angel , Vasileva-Slaveva Mariela , Kostov Stoyan , Nikolova Margarita , Said-Huntingford Ian , Ayers Duncan , Ellul Bridget , Pentimalli Francesca , Giordano Antonio , Calleja-Agius Jean
Published: June 2021

Choriocarcinoma (CC), a subtype of trophoblastic disease, is a rare and highly aggressive neoplasm. There are two main CC subtypes: gestational and non-gestational, (so called when it develops as a component of a germ cell tumor or is related to a somatic mutation of a poorly differentiated carcinoma), each with very diverse biological activity. A therapeutic approach is highly effective in patients with early-stage CC. The advanced stage of the disease also has a good prognosis with around 95% of patients cured following chemotherapy. However, advancements in diagnosis and treatment are always needed to improve outcomes for patients with CC. Long non-coding (lnc) RNAs are non-coding transcripts that are longer than 200 nucleotides. LncRNAs can act as oncogenes or tumor suppressor genes. Deregulation of their expression has a key role in tumor development, angiogenesis, differentiation, migration, apoptosis, and proliferation. Furthermore, detection of cancer-associated lncRNAs in body fluids, such as blood, saliva, and urine of cancer patients, is emerging as a novel method for cancer diagnosis. Although there is evidence for the potential role of lncRNAs in a number of cancers of the female genital tract, their role in CC is poorly understood. This review summarizes the current knowledge of lncRNAs in gestational CC and how this may be applied to future therapeutic strategies in the treatment of this rare cancer.
Tumor Vessels Fuel the Fire in Glioblastoma
Authors: Rosińska Sara , Gavard Julie
Published: June 2021

Glioblastoma, a subset of aggressive brain tumors, deploy several means to increase blood vessel supply dedicated to the tumor mass. This includes typical program borrowed from embryonic development, such as vasculogenesis and sprouting angiogenesis, as well as unconventional processes, including co-option, vascular mimicry, and transdifferentiation, in which tumor cells are pro-actively engaged. However, these neo-generated vascular networks are morphologically and functionally abnormal, suggesting that the vascularization processes are rather inefficient in the tumor ecosystem. In this review, we reiterate the specificities of each neovascularization modality in glioblastoma, and, how they can be hampered mechanistically in the perspective of anti-cancer therapies.
HGF and VEGF-A and Their Receptors Show Expression and Angiogenic Effects on Human Choroidal Endothelial Cells: Implications for Treatments of Neovascular Age-Related Macular Degeneration
Authors: Stewart Elizabeth A. , Allen Claire L. , Samaranayake Govindi J. , Stubington Thomas , Akhtar Rukhsar , Branch Matthew J. , Amoaku Winfried M.
Published: June 2021

Intraocular neovascularisation is associated with common blinding conditions including neovascular age-related macular degeneration (nAMD). Vascular endothelial growth factor (VEGF) is central in driving choroidal neovascularisation in this disease. Many clinical therapies target VEGF-A with intravitreal anti-VEGF drugs, which, however, have limited efficacy and require repeated, prolonged treatment. Other cytokines are known to be involved, including hepatocyte growth factor (HGF), which is shown to have a role in the early stages of nAMD. We investigated the effect of HGF and its co-operation with VEGF-A on human choroidal endothelial cells (CEC). The expression of HGF and related molecules in CEC was investigated using immunofluorescence, Western blotting and flow cytometry. In vitro assays for proliferation, tubule formation and migration were used to assess the potential role of HGF in neovascularisation. Primary human CEC expressed HGF, VEGF-A and their receptors MET and VEGF receptor 2 (VEGFR2). HGF increased CEC proliferation, tubule formation and migration; the increased proliferation and migration appeared to be additive with that achieved with VEGF-A. This study provides insight into growth factor co-operation in CEC signalling and indicates that simultaneous blockage of multiple growth factors or common downstream signalling pathways may provide a more sustained treatment response, enhancing treatments in nAMD.
Adaptive Multi-Scale Wavelet Neural Network for Time Series Classification
Authors: Ouyang Kewei , Hou Yi , Zhou Shilin , Zhang Ye
Published: June 2021

Wavelet transform is a well-known multi-resolution tool to analyze the time series in the time-frequency domain. Wavelet basis is diverse but predefined by manual without taking the data into the consideration. Hence, it is a great challenge to select an appropriate wavelet basis to separate the low and high frequency components for the task on the hand. Inspired by the lifting scheme in the second-generation wavelet, the updater and predictor are learned directly from the time series to separate the low and high frequency components of the time series. An adaptive multi-scale wavelet neural network (AMSW-NN) is proposed for time series classification in this paper. First, candidate frequency decompositions are obtained by a multi-scale convolutional neural network in conjunction with a depthwise convolutional neural network. Then, a selector is used to choose the optimal frequency decomposition from the candidates. At last, the optimal frequency decomposition is fed to a classification network to predict the label. A comprehensive experiment is performed on the UCR archive. The results demonstrate that, compared with the classical wavelet transform, AMSW-NN could improve the performance based on different classification networks.
Obituary: Pieter Muysken
Authors: Muntendam Antje
Published: June 2021

It is with great sadness that I write this obituary in memory of Pieter Muysken, who was a member of the editorial board of Languages [...]
Lightweight Reactive Powder Concrete Containing Expanded Perlite
Authors: Grzeszczyk Stefania , Janus Grzegorz
Published: June 2021

This paper presents the test results of the lightweight concrete properties obtained by adding expanded perlite (EP) to an RPC mix in quantities from 30% to 60% by volume of the concrete mix. It has been shown that in these cases it is possible to obtain concrete containing 30% by volume with density of approximately 1900 kg/m3 and the compressive strength > 70 MPa, with a very low water absorption value (3.3%), equal to the water absorption value of RPC without lightweight aggregate (3.3%). However, with the increased quantity of perlite (from 45% to 60%), the concrete density reduction is not observed, as the expanded perlite demonstrates very low resistance to crushing. With the increased amount of perlite, the longer periods of mixing time for all the mix components are required to obtain the homogeneous and fluid concrete mix, what causes grounding down EP. Therefore, using larger quantities of this aggregate in RPC is not recommended. The lightweight RPC shows very good freeze-thaw resistance in the presence of de-icing salt (the scaling mass is lower than 0.1 kg/m2). The above is explained by the compact microstructure of this concrete and the RPC mix location in open pores on the perlite aggregate surface, which consequently affects the strengthening of the aggregate-matrix contact without an interfacial transition zone (ITZ) visible. It has been demonstrated that pozzolanic activity of expanded perlite is much lower than the activity of silica fume and quartz powder, and its impact on increasing the RPC strength is minimal.
A Robust Numerical Methodology for Fatigue Damage Evolution Simulation in Composites
Authors: Russo Angela , Sellitto Andrea , Curatolo Prisco , Acanfora Valerio , Saputo Salvatore , Riccio Aniello
Published: June 2021

Composite materials, like metals, are subject to fatigue effects, representing one of the main causes for component collapse in carbon fiber-reinforced polymers. Indeed, when subject to low stress cyclic loading, carbon fiber-reinforced polymers exhibit gradual degradation of the mechanical properties. The numerical simulation of this phenomenon, which can strongly reduce time and costs to market, can be extremely expensive in terms of computational effort since a very high number of static analyses need to be run to take into account the real damage propagation due the fatigue effects. In this paper, a novel cycle jump strategy, named Smart Cycle strategy, is introduced in the numerical model to avoid the simulation of every single cycle and save computational resources. This cycle jump strategy can be seen as an enhancement of the empirical model proposed by Shokrieh and Lessard for the evaluation of the fatigue-induced strength and stiffness degradation. Indeed, the Smart Cycle allows quickly obtaining a preliminary assessment of the fatigue behavior of composite structures. It is based on the hypothesis that the stress redistribution, due to the fatigue-induced gradual degradation of the material properties, can be neglected until sudden fiber and/or matrix damage is verified at element/lamina level. The numerical procedure has been implemented in the commercial finite element code ANSYS MECHANICAL, by means of Ansys Parametric Design Languages (APDL). Briefly, the Smart Cycle routine is able to predict cycles where fatigue failure criteria are likely to be satisfied and to limit the numerical simulation to these cycles where a consistent damage propagation in terms of fiber and matrix breakage is expected. The proposed numerical strategy was preliminarily validated, in the frame of this research study, on 30° fiber-oriented unidirectional coupons subjected to tensile–tensile fatigue loading conditions. The numerical results were compared with literature experimental data in terms of number of cycles at failure for different percentage of the static strength. Lastly, in order to assess its potential in terms of computational time saving on more complex structures and different loading conditions, the proposed numerical approach was used to investigate the fatigue behavior of a cross-ply open-hole composite panel under tension–tension fatigue loading conditions.
Evaluating the Robustness of Biomarkers of Dairy Food Intake in a Free-Living Population Using Single- and Multi-Marker Approaches
Authors: Li Katherine J. , Burton-Pimentel Kathryn J. , Brouwer-Brolsma Elske M. , Feskens Edith J. M. , Blaser Carola , Badertscher René , Portmann Reto , Vergères Guy
Published: June 2021

Studies examining associations between self-reported dairy intake and health are inconclusive, but biomarkers hold promise for elucidating such relationships by offering objective measures of dietary intake. Previous human intervention studies identified several biomarkers for dairy foods in blood and urine using non-targeted metabolomics. We evaluated the robustness of these biomarkers in a free-living cohort in the Netherlands using both single- and multi-marker approaches. Plasma and urine from 246 participants (54 ± 13 years) who completed a food frequency questionnaire were analyzed using liquid and gas chromatography-mass spectrometry. The targeted metabolite panel included 37 previously-identified candidate biomarkers of milk, cheese, and/or yoghurt consumption. Associations between biomarkers and energy-adjusted dairy food intakes were assessed by a ‘single-marker’ generalized linear model, and stepwise regression was used to select the best ‘multi-marker’ panel. Multi-marker models that also accounted for common covariates better captured the subtle differences for milk (urinary galactose, galactitol; sex, body mass index, age) and cheese (plasma pentadecanoic acid, isoleucine, glutamic acid) over single-marker models. No significant associations were observed for yogurt. Further examination of other facets of validity of these biomarkers may improve estimates of dairy food intake in conjunction with self-reported methods, and help reach a clearer consensus on their health impacts.
Recycling of Spent Reverse Osmosis Membranes for Second Use in the Clarification of Wet-Process Phosphoric Acid
Authors: Khaless Khaoula , Achiou Brahim , Boulif Rachid , Benhida Rachid
Published: June 2021

Various techniques have been used to “clean-up” wet-process phosphoric acid such as precipitation, flotation and adsorption. To address the potential of membrane processes in the phosphoric acid clarification process, this study explores the benefits of membrane techniques as a green separation technique for phosphoric acid clarification in an eco-efficient way through the use of recycling spent reverse osmosis membrane. Regenerated membrane was used to study the phosphoric acid clarification at a laboratory scale. They were immersed in an oxidizer for at most seven days. The samples were characterized systematically before immersion in an oxidant media. In this study, the potential to regenerate spent membranes and application of this media to clarify the 29% P2O5 phosphoric acid was demonstrated. This study shows, through experiments, that the reverse osmosis (RO) membranes could achieve a rejection of 70% and 61% for suspended solid and organic matter, respectively. These promising results will pave the way for implementation of these membranes in phosphoric acid treatment. Moreover, besides being economically advantageous, the use of the spent membrane is likely an environmentally friendly route (no waste, no organic solvent and effluent to be regenerated later on).
Source Rock Evaluation and Hydrocarbon Generation Model of a Permian Alkaline Lakes—A Case Study of the Fengcheng Formation in the Mahu Sag, Junggar Basin
Authors: Tang Yong , He Wenjun , Bai Yubin , Zhang Xiang , Zhao Jingzhou , Yang Sen , Wu Heyuan , Zou Yang , Wu Weitao
Published: June 2021

The alkaline lake source rocks of the Fengcheng Formation are developed in the Mahu Sag of the Junggar Basin. Different from traditional continental fresh water and saltwater lake source rocks, alkaline lake source rocks lack targeted evaluation criteria, and it is unknown whether their hydrocarbon generation models are consistent with traditional models. Therefore, in the present study, evaluation standards and hydrocarbon generation models of alkaline lake source rocks are discussed based on geological and organic geochemical data and a systematic summary of the geochemical characteristics of the Fengcheng Formation source rocks. The Fengcheng Formation source rocks are mainly diamictite with mixed argillaceous rock and dolomite; most total organic carbon (TOC) values range from 0.2–1.4%; and the kerogen is primarily oil-prone type II, reaching low- to high-maturity stages. Based on the types of organic matter in source rocks and the relationships between organic matter abundance parameters, the evaluation standard of alkaline lake source rocks is proposed. The Fengcheng Formation is mainly composed of good to excellent source rocks (55.5%) with high hydrocarbon generation potential. The single-peak hydrocarbon generation model of the Fengcheng Formation is similar to that of traditional freshwater or saltwater lakes, with a high hydrocarbon generation rate, two to five times that of the traditional model; its main particularity is in the formation of naphthenic crude oil from the kerogen of bacteria and algae. A new understanding of the hydrocarbon generation potential and model of alkaline lake source rocks in the Fengcheng Formation can provide support for tight oil and shale oil exploration in the Mahu Sag.
Erratum: Elshafie et al. Biological and Spectroscopic Investigations of New Tenoxicam and 1.10-Phenthroline Metal Complexes. Molecules 2020, 25, 1027
Authors: Elshafie Hazem S. , Sadeek Sadeek A. , Camele Ippolito , Awad Hanem M. , Mohamed Amira A.
Published: June 2021

The authors of this paper [...]
Furanocoumarins from Ruta chalepensis with Amebicide Activity
Authors: Bazaldúa-Rodríguez Aldo Fabio , Quintanilla-Licea Ramiro , Verde-Star María Julia , Hernández-García Magda Elizabeth , Vargas-Villarreal Javier , Garza-González Jesús Norberto
Published: June 2021

Entamoeba histolytica (protozoan; family Endomoebidae) is the cause of amoebiasis, a disease related to high morbidity and mortality. Nowadays, this illness is considered a significant public health issue in developing countries. In addition, parasite resistance to conventional medicinal treatment has increased in recent years. Traditional medicine around the world represents a valuable source of alternative treatment for many parasite diseases. In a previous paper, we communicated about the antiprotozoal activity in vitro of the methanolic (MeOH) extract of Ruta chalepensis (Rutaceae) against E. histolytica. The plant is extensively employed in Mexican traditional medicine. The following workup of the MeOH extract of R. chalepensis afforded the furocoumarins rutamarin (1) and chalepin (2), which showed high antiprotozoal activity on Entamoeba histolytica trophozoites employing in vitro tests (IC50 values of 6.52 and 28.95 µg/mL, respectively). Therefore, we offer a full scientific report about the bioguided isolation and the amebicide activity of chalepin and rutamarin.
Effect of Atmospheric-Pressure Plasma Treatments on Fracture Toughness of Carbon Fibers-Reinforced Composites
Authors: Kim Won-Jong , Heo Young-Jung , Lee Jong-Hoon , Rhee Kyong Yop , Park Soo-Jin
Published: June 2021

In this study, nano-scale fillers are added to epoxy matrix-based carbon fibers-reinforced composites (CFRPs) to improve the mechanical properties of multi-scale composites. Single-walled carbon nanotubes (SWCNTs) used as nano-scale fillers are treated with atmospheric-pressure plasma to introduce oxygen functional groups on the fillers’ surface to increase the surface free energy and polar component, which relates to the mechanical properties of multi-scale composites. In addition, the effect of dispersibility was analyzed through the fracture surfaces of multi-scale composites containing atmospheric-pressure plasma-treated SWCNTs (P-SWCNTs) under high load conditions. The fillers content has an optimum weight percent load at 0.5 wt.% and the fracture toughness (KIC) method is used to demonstrate an improvement in mechanical properties. Here, KIC was calculated by three equations based on different models and we analyzed the correlation between mechanical properties and surface treatment. Compared to the composites of untreated SWCNTs, the KIC value is improved by 23.7%, suggesting improved mechanical properties by introducing selective functional groups through surface control technology to improve interfacial interactions within multi-scale composites.
Efficient Generation of Microdroplets Using Tail Breakup Induced with Multi-Branch Channels
Authors: Tanaka Daiki , Kajiya Satsuki , Shijo Seito , Yoon Dong Hyun , Furuya Masahiro , Nozaki Yoshito , Fujita Hiroyuki , Sekiguchi Tetsushi , Shoji Shuichi
Published: June 2021

In recent years, research on the application of microdroplets in the fields of biotechnology and chemistry has made remarkable progress, but the technology for the stable generation of single-micrometer-scale microdroplets has not yet been established. In this paper, we developed an efficient and stable single-micrometer-scale droplet generation device based on the fragmentation of droplet tails, called “tail thread mode”, that appears under moderate flow conditions. This method can efficiently encapsulate microbeads that mimic cells and chemical products in passively generated single-micrometer-scale microdroplets. The device has a simple 2D structure; a T-junction is used for droplet generation; and in the downstream, multi-branch channels are designed for droplet deformation into the tail. Several 1–2 µm droplets were successfully produced by the tail’s fragmentation; this continuous splitting was induced by the branch channels. We examined a wide range of experimental conditions and found the optimal flow rate condition can be reduced to one-tenth compared to the conventional tip-streaming method. A mold was fabricated by simple soft lithography, and a polydimethylsiloxane (PDMS) device was fabricated using the mold. Based on the 15 patterns of experimental conditions and the results, the key factors for the generation of microdroplets in this device were examined. In the most efficient condition, 61.1% of the total droplets generated were smaller than 2 μm.
Magnesium Hydroxide Nanoparticles Kill Exponentially Growing and Persister Escherichia coli Cells by Causing Physical Damage
Authors: Nakamura Yohei , Okita Kaede , Kudo Daisuke , Phuong Dao Nguyen Duy , Iwamoto Yoshihito , Yoshioka Yoshie , Ariyoshi Wataru , Yamasaki Ryota
Published: June 2021

Magnesium hydroxide nanoparticles are widely used in medicinal and hygiene products because of their low toxicity, environment-friendliness, and low cost. Here, we studied the effects of three different sizes of magnesium hydroxide nanoparticles on antibacterial activity: NM80, NM300, and NM700. NM80 (D50 = 75.2 nm) showed a higher bactericidal effect against Escherichia coli than larger nanoparticles (D50 = 328 nm (NM300) or 726 nm (NM700)). Moreover, NM80 showed a high bactericidal effect against not only exponential cells but also persister cells, which are difficult to eliminate owing to their high tolerance to antibiotics. NM80 eliminated strains in which magnesium-transport genes were knocked out and exhibited a bactericidal effect similar to that observed in the wild-type strain. The bactericidal action involved physical cell damage, as confirmed using scanning electron microscopy, which showed that E. coli cells treated with NM80 were directly injured.
Carrier Fibers for the Safe Dosage of Nanoparticles in Nanocomposites: Nanomechanical and Thermomechanical Study on Polycarbonate/Boehmite Electrospun Fibers Embedded in Epoxy Resin
Authors: Cano Murillo Natalia , Ghasem Zadeh Khorasani Media , Silbernagl Dorothee , Emamverdi Farnaz , Cacua Karen , Hodoroaba Vasile-Dan , Sturm Heinz
Published: June 2021

The reinforcing effect of boehmite nanoparticles (BNP) in epoxy resins for fiber composite lightweight construction is related to the formation of a soft but bound interphase between filler and polymer. The interphase is able to dissipate crack propagation energy and consequently increases the fracture toughness of the epoxy resin. Usually, the nanoparticles are dispersed in the resin and then mixed with the hardener to form an applicable mixture to impregnate the fibers. If one wishes to locally increase the fracture toughness at particularly stressed positions of the fiber-reinforced polymer composites (FRPC), this could be done by spraying nanoparticles from a suspension. However, this would entail high costs for removing the nanoparticles from the ambient air. We propose that a fiber fleece containing bound nanoparticles be inserted at exposed locations. For the present proof-of-concept study, an electrospun polycarbonate nonwoven and taurine modified BNP are proposed. After fabrication of suitable PC/EP/BNP composites, the thermomechanical properties were tested by dynamic mechanical analysis (DMA). Comparatively, the local nanomechanical properties such as stiffness and elastic modulus were determined by atomic force microscopy (AFM). An additional investigation of the distribution of the nanoparticles in the epoxy matrix, which is a prerequisite for an effective nanocomposite, is carried out by scanning electron microscopy in transmission mode (TSEM). From the results it can be concluded that the concept of carrier fibers for nanoparticles is viable.