bluuebunny/crossref_metadata_2025 huggingface dataset:
Long-Time Prediction of Arrhythmic Cardiac Action Potentials Using Recurrent Neural Networks and Reservoir Computing
Authors: Shahi Shahrokh , Marcotte Christopher D. , Herndon Conner J. , Fenton Flavio H. , Shiferaw Yohannes , Cherry Elizabeth M.
Published: September 2021

The electrical signals triggering the heart's contraction are governed by non-linear processes that can produce complex irregular activity, especially during or preceding the onset of cardiac arrhythmias. Forecasts of cardiac voltage time series in such conditions could allow new opportunities for intervention and control but would require efficient computation of highly accurate predictions. Although machine-learning (ML) approaches hold promise for delivering such results, non-linear time-series forecasting poses significant challenges. In this manuscript, we study the performance of two recurrent neural network (RNN) approaches along with echo state networks (ESNs) from the reservoir computing (RC) paradigm in predicting cardiac voltage data in terms of accuracy, efficiency, and robustness. We show that these ML time-series prediction methods can forecast synthetic and experimental cardiac action potentials for at least 15–20 beats with a high degree of accuracy, with ESNs typically two orders of magnitude faster than RNN approaches for the same network size.
Investigation of Distinctive Morpho-Physio and Biochemical Alterations in Desi Chickpea at Seedling Stage Under Irrigation, Heat, and Combined Stress
Authors: Jameel Saima , Hameed Amjad , Shah Tariq Mahmud
Published: September 2021

Global climatic instabilities have become the main reason for drastic yield losses in chickpea. This shift in climate could be a great threat in the future for food security in developing countries. Chickpea production is badly hampered by heat stress coupled with drought stress, and these factors can reduce yields by 40–45%. To mitigate yield losses due these abiotic factors, irrigation supplementation could be the best strategy. The present study aimed to (i) investigate the tolerance response of 9 desi chickpea genotypes against heat stress (H), irrigation (I), and a combination of both (I+H) through morphophysiological and biochemical indices at early growth stage, and (ii) assess yield performance across multiple locations of the country. Results revealed that under irrigation treatment, all genotypes perform well, but the genotypes D-09027 and D-09013 showed best performance because, as compared to control, they retained root length, seedling fresh weight, root fresh weight, root dry weight, esterase activity, Malondialdehyde (MDA) content, total chlorophyll, and total carotenoids. Shoot length and total phenolic contents (TPC) increased in both genotypes. Superoxide dismutase (SOD) and peroxidase (POD) increased in D-09027 and retained in D-09013. Catalase activity increased in D-09013 and retained in D-09027. Protease activity, total water potential and osmotic potential decreased in both genotypes and depicted high yield potential with 27 and 30% increase in yield over Bhakhar-2011 (check), respectively. In case of heat stress, maximum tolerance was found in genotypes CH104/06 and D-09013 with no change in shoot and root length, seedling dry weight, shoot fresh and dry weight, root dry weight, relative water content, turgor water potential, catalase (CAT) activity, esterase activity, increased root fresh weight, peroxidase activity (POD), ascorbate peroxidase activity (APX), and lycopene with low accumulation of protease and Malondialdehyde content (MDA). Both genotypes depicted high yield potential with 30 and 43% increase in yield over check across multiple locations of the country. Under the combined treatment, most genotypes showed good performance, while CH104/06 was selected as best performer genotype because significant of its increased root fresh weight, lycopene content, chlorophyll b, total carotenoids, total chlorophyll, retained shoot length, root length, seedling fresh and dry weight, total water potential, osmotic potential, relative water content, peroxidase activity (POD), catalase, esterase, and its ascorbate peroxidase (APX) activity and total soluble proteins (TSP) showed highest yield potential with 43% increase over check. Identified best performing and tolerant genotypes can further be employed for breeding climate-smart chickpea genotypes for sustainable production under changing climate.
Phosphorus-Use Efficiency Modified by Complementary Effects of P Supply Intensity With Limited Root Growth Space
Authors: Gong Haiqing , Kabeto Wako Bilisuma , Xiang Yue , Jiao Xiaoqiang
Published: September 2021

Space availability and the maintenance of adequate phosphorus (P) supply in the root zone are essential for achieving high yield and P-use efficiency in maize production by manipulating the root morphology and arbuscular mycorrhizal (AM) fungi colonization. A major trade-off exists between root growth and AM colonization that is influenced by soil P supply intensity and space availability. However, how soil P manipulates the root morphological characteristics and AM colonization to compensate for the limitation of root-growth space induced by high-planting density is not clear. Therefore, pot experiments were conducted to investigate interactions between the root growth and AM fungi by optimizing soil P supply to compensate for limited root growth space induced by high-planting density. Similar shoot biomass and P uptake values were obtained in P200 (200 mg P kg−1 soil) under D = 40 (i.e., diameter of the pot is 40 cm) and P400 under D = 30, and similar values were obtained for root length, tap root length, root angle, lateral root density, and AM colonization. However, the improvement in P supply in the root zone, shoot biomass, and P uptake in P400 under D = 20 were lower than in P200 under D = 30, and there were no significant differences in the root parameters between P200 and P400 under D = 20; similarly, the root growth and AM colonization exhibited similar trends. These results suggest that optimizing P supply in the root zone to regulate the interaction between root morphological traits and AM colonization can compensate for limited root-growth space. Although P supply in the root zone increased after the root-growth space was compressed, it could not meet the P demand of maize; thus, to achieve the most efficient use of P under intensive high-density maize production, it is necessary to optimally coordinate root growth space and P supply in the root zone.
Climate Change and Emotions: Analysis of People’s Emotional States in Southern Ecuador
Authors: Iniguez-Gallardo Verónica , Lenti Boero Daniela , Tzanopoulos Joseph
Published: September 2021

Climate change involves multiple emotional expressions associated with specific labels, notably: ‘concern,’ ‘guilt,’ or ‘scepticism.’ However, there are other types of emotions that have been less analysed, such as ‘powerlessness,’ ‘anger’ and ‘confusion’ that are of equal importance for predicting behavioural changes toward this climatic issue. Likewise, few studies in this research field rely on qualitative data to understand and identify the causative agents for the emotional arousal. This research explores a range of emotions, mixing those that have been widely studied and those that have been hardly analysed. It also looks at the demographic parameters associated with such emotions using a population sample from southern Ecuador. The study analyses quantitative and qualitative data gathered through structured-questionnaires whereby participants were given agency to select and define how they themselves sense emotionally climate change. The results indicate that two of the five participants’ most selected emotions are shared with other nations (‘concern,’ ‘guilt’), while the other three have been less reported and studied in the climate change field (‘powerlessness,’ ‘anger,’ and ‘confusion’). These emotions were found to be aroused by different reasons associated with specific demographic variables. The findings reveal the role of the cultural and local environment in the emotional arousal and its relevance for designing more effective climate communication campaigns.
Nature Experiences of Older People for Active Ageing: An Interdisciplinary Approach to the Co-Design of Community Gardens
Authors: Boffi Marco , Pola Linda , Fumagalli Natalia , Fermani Elisabetta , Senes Giulio , Inghilleri Paolo
Published: September 2021

In Western countries, the concepts of healthy ageing and active ageing, that is, concepts that favour health, participation, and security to increase the quality of life of older people, have become key issues. Hence, the effective design of public green spaces in urban areas is crucial, as visiting these areas encourages social relations and interactions in natural, healthy contexts. Consistent with these perspectives, existing landscape design criteria emphasise the importance of considering not only the functional and aesthetic elements, but also the ecosystemic and biophilic relationships between people and the environment, producing positive effects for both. To maximise the impact of such design criteria, proper engagement strategies are desirable, both to assign an active role to older people themselves and to inform the fine-tuning of the design process according to the specific needs of the local population. This study presents an interdisciplinary co-design method that encourages and informs a biophilic approach by describing the experiences of people in natural environments, actual and designed, through the application of attention restoration theory (ART). The case study was developed through six focus group meetings with older people for the co-design of a restorative area in a community garden in the Ortica district in Milan (Italy). Results show how the main needs expressed by participants can be classified into the ART factors of “compatibility” (a multifunctional garden), “fascination” (sense of contact with nature), and “being away” (metaphorical escape from nursing homes). The garden designed includes biophilic principles that respond to such needs, and specific links with designed elements were identified. For example, “being away” (e.g., isolation from daily routine and visual occlusion of the surroundings) and “compatibility” (e.g., pergola and aesthetic value) are the factors that include the elements that more satisfactorily answer previous needs. “Fascination” also includes many positive aspects, allowing space for improvements (e.g., more water elements, interaction with animals). Implications of the method are discussed, including the importance of subjective experience in informing design, the use of different psychological constructs to describe it, and the methodological alternatives for psychological assessment.
Differential Effects of Mindfulness-Based Intervention Programs at Work on Psychological Wellbeing and Work Engagement
Authors: Calcagni Cristián Coo , Salanova Marisa , Llorens Susana , Bellosta-Batalla Miguel , Martínez-Rubio David , Martínez Borrás Rosa
Published: September 2021

Two different mindfulness-based interventions were deployed in a sample of white-collar workers to explore the differential effects on different facets of mindfulness, dimensions of psychological wellbeing, work engagement, performance, and stress of a participant. A total of 28 participants completed one of the different programs, and their results were compared between groups and against 27 participants randomly allocated to a waiting list control group. Results suggest both mindfulness intervention programs were successful at increasing the levels of psychological wellbeing, work engagement, and performance of the participants, as well as decreasing their levels of stress. Significant differences were found between the two programs in all outcome variables. Results suggest that brief and customized mindfulness interventions at work are as successful as lengthier programs.
MDMA-Assisted Therapy as a Means to Alter Affective, Cognitive, Behavioral, and Neurological Systems Underlying Social Dysfunction in Social Anxiety Disorder
Authors: Luoma Jason , Lear M. Kati
Published: September 2021

Social anxiety disorder (SAD) is a prevalent and often debilitating psychiatric disorder that can assume a chronic course even when treated. Despite the identification of evidence-based pharmacological and behavioral treatments for SAD, much room for improved outcomes exists and 3,4-methylenedioxymethamphetamine (MDMA) has been proposed as a promising adjunctive treatment to psychological interventions for disorders characterized by social dysfunction. A small randomized, placebo-controlled trial of MDMA-assisted therapy (MDMA-AT) for social anxiety in autistic adults offered encouraging results, but more research is sorely needed to explore the potential for MDMA-AT in treating SAD. This review aims to stimulate future study by summarizing research on disruptions in neurological, perceptual, receptive, and expressive systems regulating social behavior in SAD and proposing how MDMA-AT may alter these systems across four domains. First, we review research highlighting the roles of social anhedonia and reduced social reward sensitivity in maintaining SAD, with specific attention to the reduction in positive affect in social situations, infrequent social approach behaviors, and related social skills deficits. We posit that MDMA-AT may enhance motivation to connect with others and alter perceptions of social reward for an extended period following administration, thereby potentiating extinction processes, and increasing the reinforcement value of social interactions. Second, we review evidence for the central role of heightened social evaluative threat perception in the development and maintenance of SAD and consider how MDMA-AT may enhance experiences of affiliation and safety when interacting with others. Third, we consider the influence of shame and the rigid application of shame regulation strategies as important intrapersonal processes maintaining SAD and propose the generation of self-transcendent emotions during MDMA sessions as a mechanism of shame reduction that may result in corrective emotional experiences and boost memory reconsolidation. Finally, we review research on the role of dysfunctional interpersonal behaviors in SAD that interfere with social functioning and, in particular, the development and maintenance of close and secure relationships. We discuss the hypothesized role of MDMA-AT in improving social skills to elicit positive interpersonal responses from others, creating a greater sense of belonging, acceptance, and social efficacy.
Identifying Distinguishable Clinical Profiles Between Single Suicide Attempters and Re-Attempters
Authors: Lübbert Marlehn , Bahlmann Lydia , Josfeld Sebastian , Bürger Jessica , Schulz Alexandra , Bär Karl-Jürgen , Polzer Udo , Walter Martin , Kastner Ulrich W. , Sobanski Thomas , Wagner Gerd
Published: September 2021

More than 800,000 individuals die from suicide each year in the world, which has a devastating impact on families and society. Ten to twenty times more attempt suicide. Previous studies showed that suicide attempters represent a heterogeneous group regarding demographic characteristics, individual characteristics of a suicidal attempt, and the assumed clinical factors, e.g., hopelessness or impulsivity, thus differently contributing to the likelihood of suicidal behavior. Therefore, in the present study, we aim to give a comprehensive clinical description of patients with repeated suicide attempts compared to single attempters. We explored putative differences between groups in clinical variables and personality traits, sociodemographic information, and specific suicide attempt-related information. A sample of patients with a recent suicide attempt (n= 252), defined according to DSM-5 criteria for a suicidal behavior disorder (SBD), was recruited in four psychiatric hospitals in Thuringia, Germany. We used a structured clinical interview to assess the psychiatric diagnosis, sociodemographic data, and to collect information regarding the characteristics of the suicide attempt. Several clinical questionnaires were used to measure the suicide intent and suicidal ideations, depression severity, hopelessness, impulsivity, aggression, anger expression, and the presence of childhood trauma. Univariate and multivariate statistical methods were applied to evaluate the postulated risk factors and, to distinguish groups based on these measures. The performed statistical analyses indicated that suicide attempters represent a relatively heterogeneous group, nevertheless associated with specific clinical profiles. We demonstrated that the re-attempters had more severe psychopathology with significantly higher levels of self-reported depression, suicidal ideation as well as hopelessness. Furthermore, re-attempters had more often first-degree relatives with suicidal behavior and emotional abuse during childhood. They also exhibited a higher degree of specific personality traits, i.e., more “urgency” as a reaction to negative emotions, higher excitability, higher self-aggressiveness, and trait anger. The multivariate discriminant analysis significantly discriminated the re-attempters from single attempters by higher levels of self-aggressiveness and suicidal ideation. The findings might contribute to a better understanding of the complex mechanisms leading to suicidal behavior, which might improve the early identification and specific treatment of subjects at risk for repeated suicidal behavior.
Short-Term Association of Air Pollutant Levels and Hospital Admissions for Stroke and Effect Modification by Apparent Temperature: Evidence From Shanghai, China
Authors: Weng Lvkan , Li Na , Feng Tienan , Zhu Rongjia , Zheng Zhi-Jie
Published: September 2021

The epidemiological evidence on relationships between air pollution, temperature, and stroke remains inconclusive. Limited evidence is available for the effect modification by apparent temperature, an indicator reflecting reactions to the thermal environment, on short-term associations between air pollution and hospital admissions for stroke. We used a generalized additive model with Poisson regression to estimate the relative risk (RR) of stroke admissions in Shanghai, China, between 2014 and 2016 associated with air pollutants, with subgroup analyses by age, sex, apparent temperature, and season. During the study period, changes in the daily number of stroke admissions per 10 μg/m3 increase in nitrogen dioxide (at lags 0, 1, 0–1, and 0–2) ranged from 1.05 (95% CI: 0.82%, 2.88%) to 2.24% (95% CI: 0.84%, 3.65%). For each 10 μg/m3 increase in sulfur dioxide concentrations at lags 1, 2, 0–1, and 0–2, the RR of daily stroke admissions increased by 3.34 (95% CI: 0.955%, 5.79%), 0.32 (95% CI: −1.97%, 2.67%), 3.33 (95% CI: 0.38%, 6.37%), and 2.86% (95% CI: −0.45%, 6.28%), respectively. The associations of same-day exposure to nitrogen dioxide with stroke admissions remained significant after adjustment for ozone levels. These associations were not modified by sex, age, apparent temperature, or season. More research is warranted to determine whether apparent temperature modifies the associations between air pollution and stroke admissions.
Long-Term Course of Humoral and Cellular Immune Responses in Outpatients After SARS-CoV-2 Infection
Authors: Schiffner Julia , Backhaus Insa , Rimmele Jens , Schulz Sören , Möhlenkamp Till , Klemens Julia Maria , Zapf Dorinja , Solbach Werner , Mischnik Alexander
Published: September 2021

Characterization of the naturally acquired B and T cell immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is important for the development of public health and vaccination strategies to manage the burden of COVID-19 disease. We conducted a prospective, cross-sectional analysis in COVID-19 recovered patients at various time points over a 10-month period in order to investigate how circulating antibody levels and interferon-gamma (IFN-γ) release by peripheral blood cells change over time following natural infection. From March 2020 till January 2021, we enrolled 412 adults mostly with mild or moderate disease course. At each study visit, subjects donated peripheral blood for testing of anti-SARS-CoV-2 IgG antibodies and IFN-γ release after SARS-CoV-2 S-protein stimulation. Anti-SARS-CoV-2 immunoglobulin G (IgG) antibodies were positive in 316 of 412 (76.7%) and borderline in 31 of 412 (7.5%) patients. Our confirmation assay for the presence of neutralizing antibodies was positive in 215 of 412 (52.2%) and borderline in 88 of 412 (21.4%) patients. Likewise, in 274 of 412 (66.5%) positive IFN-γ release and IgG antibodies were detected. With respect to time after infection, both IgG antibody levels and IFN-γ concentrations decreased by about half within 300 days. Statistically, production of IgG and IFN-γ were closely associated, but on an individual basis, we observed patients with high-antibody titres but low IFN-γ levels and vice versa. Our data suggest that immunological reaction is acquired in most individuals after natural infection with SARS-CoV-2 and is sustained in the majority of patients for at least 10 months after infection after a mild or moderate disease course. Since, so far, no robust marker for protection against COVID-19 exists, we recommend utilizing both, IgG and IFN-γ release for an individual assessment of the immunity status.
The Estimated Effect of Physicians' Advice for Smoking Cessation and Assumed Tobacco Retail Price Increase on Smoker's Intention to Quit in Shanghai, China: A Cross-Sectional Study
Authors: Wang Ruiping , Gao Xiangjin , Qiang Yan , Yang Qiong , Li Xiaopan , Li Bin
Published: September 2021

Background: Tobacco consumption produces a heavy disease burden worldwide, and tobacco price increase, an advertisement for tobacco-induced harm, graphic warning labels on cigarette packages and advice of physicians for quitting are policies that have been proved as effective smoking cessation measures. But evidence on the estimated effect of advice of physicians for quitting and assumed tobacco retail price increase on smoking cessation intention among smokers is still limited in China.

Methods: From January to April of 2021, we recruited 664 current smokers in Songjiang district of Shanghai by a multistage sampling design. We implemented a logistic regression analysis to calculate the odds ratio (OR) and 95% confidence interval (CI) to explore how smoking cessation intention would be influenced by the assumed tobacco retail price increase as well as advice of physicians for quitting, and used the paired tabulation method to identify the salient tobacco control measures among smokers as well.

Results: A total of 664 current smokers included 548 males (82.53%), with an average smoking duration of 22.50 years (SD: 11.52 years). About 68.79 and 43.67% of current smokers reported smoking cessation intention due to advice of physicians for quitting and the assumed tobacco retail price increase, respectively. Logistic regression analysis indicated that female smokers (OR = 2.85 and 4.55), smokers with previous smoking cessation attempt (OR = 3.71 and 3.07), longer smoking duration (OR = 2.26 and 2.68), lower smoking intensity (OR = 1.82 and 1.69), and heavier tobacco burdens (OR = 1.67 and 2.22) had the higher intention of smoking cessation both due to advice of physicians for quitting and due to assumed tobacco price increase, respectively. Meanwhile, the advice of physicians for quitting was more effective and acceptable (over 80%) than the assumed tobacco price increase for inducing smokers to consider quitting in Shanghai.

Conclusions: Smokers have a high intention of smoking cessation in Shanghai, and the advice of physicians for quitting is a potentially more salient tobacco control measure than the assumed tobacco retail price increase. Incorporating smoking duration, intensity, personal burden as well as noncommunicable disease (NCD) status of smokers into the implementation of tobacco control measures is beneficial for descending smoking prevalence.
Evidence that C/EBP-β LAP Increases Fat Metabolism and Protects Against Diet-Induced Obesity in Response to mTOR Inhibition
Authors: Bitto Alessandro , Tatom Nicole , Krivak Thomas , Grotz Peter , Kaeberlein Matt
Published: September 2021

Aging and obesity are common risk factors for numerous chronic pathologies, and the compounding effects of old age and increased adiposity pose a serious threat to public health. Starting from the assumption that aging and obesity may have shared underpinnings, we investigated the antiobesogenic potential of a successful longevity intervention, the mTORC1 inhibitor rapamycin. We find that rapamycin prevents diet-induced obesity in mice and increases the activity of C/EBP-β LAP, a transcription factor that regulates the metabolic shift to lipid catabolism observed in response to calorie restriction. Independent activation of C/EBP-β LAP with the antiretroviral drug adefovir dipivoxil recapitulates the anti-obesogenic effects of rapamycin without reducing signaling through mTORC1 and increases markers of fat catabolism in the liver. Our findings support a model that C/EBP-β LAP acts downstream of mTORC1 signaling to regulate fat metabolism and identifies a novel drug that may be exploited to treat obesity and decrease the incidence of age-related disease.
CD123-Directed Bispecific Antibodies for Targeting MDS Clones and Immunosuppressive Myeloid-Derived Suppressor Cells (MDSC) in High-Risk Adult MDS Patients
Authors: Uckun Fatih M. , Watts Justin
Published: September 2021

There is an urgent need to identify effective strategies to prevent leukemic transformation and induce sustained deep remissions in adult high-risk myelodysplastic syndrome (MDS) patients. This article discusses the clinical impact potential of bispecific antibodies (BiAB) capable of redirecting host T-cell cytotoxicity in an MHC-independent manner to malignant clones as well as immunosuppressive myeloid-derived suppressor cells (MDSC) as a new class of anti-MDS drug candidates. T-cell engaging BiAB targeting the CD123 antigen may help delay disease progression in high-risk adult MDS and potentially reduce the risk of transformation to secondary AML.
The Role of Poetry in Promoting a Sustainability Mindset: Walter Benjamin as a Guide Toward a Slow Journey
Authors: Molderez Ingrid , Baraniuk Diana , Lambrechts Wim
Published: September 2021

The emergence of highly complex sustainability challenges in modern society has led to the necessity of searching for more effective approaches to education for sustainable development. Research has shown that reflection leads toward more profound levels of engagement with respect to sustainable actions. Therefore, higher education has a role to play in stimulating reflection in light of sustainability. Art-based techniques, which have not been included alongside traditional teaching methods, have begun to gain the attention of researchers and teachers in higher education as they produce a deeper impact and involvement and can have a positive influence on the minds and hearts of the students. The aim of this paper is to demonstrate that poetry can contribute to integrating the arts and humanities in management education. The potential effect of poetry on business management majors is being explored as a part of their Corporate Social Responsibility course. Poetry has considerable potential as an innovative approach to teach sustainability, but it is rather unusual in business education. Poetry was chosen as an enabler for reflection and emotions. This original teaching project was followed by a research project relying on reflective assignments. A rereading of Walter Benjamin'sIlluminationsfrom the perspective of sustainability studies was a source of inspiration, in particular “Theses on the Philosophy of History,” “The Storyteller” and “Unpacking My Library.” The paper assumes that Benjamin's ideas relate to a slow journey involving “awakening,” “wisdom” and “in a process,” three elements that are at the core of promoting a sustainability mindset. The research project consisted of four reflection assignments students had to comply with: reading and interpreting poetry; searching for a poem which would be most appropriate for the discussed sustainability topic; creating their own poem and reflecting on the whole task. The research took place in the second semester of 2020 and first semester of 2021, all in COVID-19 pandemic context. Students' participation was not mandatory, but the majority joined. Their perceptions and impressions reinforce the existing knowledge about the emotional power of poetry to encourage reflection. The results show that poetry plays a relevant role in encouraging future managers to develop a frame of mind that incorporates sustainability and responsibility. Business students are open to this approach because it adds a new and unexpected dimension to their studies. Despite the urge to integrate reflections, this is still an exception for the majority of management courses. The results suggest that poetry is a relevant instrument to promote a more sustainable mindset among future managers. Paradoxically, by emphasizing a slow journey, i.e., allowing time for integrating reflective practices, a transition toward sustainability in daily managerial processes can be accelerated.
Small Bowel Perforation Due to Blunt Trauma of Left Leg With an Incarcerated Inguinal Hernia: A Case Report
Authors: Shao Jianli , Sun Long , Fu Qinghui
Published: September 2021

We report a rare case of a 77-year-old man with a known left inguinal hernia presenting with groin pain following a blunt trauma of the left leg. Diagnosis of small bowel perforation away from the hernia was obtained only in surgery. Difficulty in preoperative diagnosis, rarity of histologic pattern, and surgical challenges make this case very interesting for surgeons and radiologists. Our conclusion upon dealing with the situation is that the diagnosis of small bowel perforation following blunt injury to a non-abdominal trauma is rare and difficult. The association between inguinal hernia and non-abdominal trauma may result in small bowel injuries that normally do not appear. Therefore, clinicians should be cautious in treating non-abdominal trauma patients with inguinal hernias.
Cardiovascular Disease in the Peruvian Andes: Local Perceptions, Barriers, and Paths to Preventing Chronic Diseases in the Cajamarca Region
Authors: Sanchez-Samaniego Giuliana , Hartinger Stella M. , Tallman Paula S. , Mäusezahl Daniel
Published: September 2021

Objectives: Public health interventions can be improved by understanding peoples’ explanatory models of disease. We explore awareness and perceptions of cardiovascular diseases (CVD) and options for preventative actions in young adults living in rural Andean communities.

Methods: We used convenience sampling to select 46 men and women from communities in Cajamarca (Peru). Subjects participated in eight focus groups where they discussed their understanding and perceived causes of CVD as well as barriers and pathways to healthy lifestyles.

Results: Fresh foods, physical activity, unpleasant emotions, and healthcare access were cited as important determinants of healthy lifestyles. Barriers to healthy diets included lacking nutritional knowledge, fluctuating food prices, and limited access to foodstuffs. Women felt particularly vulnerable to CVD and identified gendered barriers to manage stress and engage in sports. Low health literacy, poor doctor-patient relationships, and long distances prevented participants from fully accessing healthcare.

Conclusion: CVD prevention interventions should consider local knowledge of these diseases and of healthy lifestyles, and harness ongoing programmes that have successfully promoted good nutrition in children and pregnant women. In concert with public-private parterships, governments should include disease prevention interventions for the entire family.
Precision Oncology in Metastatic Uterine Cancer; Croatian First-Year Experience of the Comprehensive Genomic Profiling in Everyday Clinical Practice
Authors: Čerina Dora , Matković Višnja , Katić Kristina , Lovasić Ingrid Belac , Šeparović Robert , Canjko Ivana , Jakšić Blanka , Fröbe Ana , Pleština Stjepko , Bajić Žarko , Vrdoljak Eduard
Published: September 2021

Comprehensive genomic profiling (CGP) is gradually becoming an inevitable part of the everyday oncology clinical practice. The interpretation and optimal implementation of the results is one of the hot topics of modern-day oncology. According to the recent findings, uterine cancer harbors a high level of gene alterations but is still insufficiently explored. The primary goal of this project was to assess the proportion of patients with targetable mutations. Also, the aim was to define and emphasize potential opportunities as well as the problems we have faced in the first year of testing on the national level. We performed a multicentric, retrospective, nested cross-sectional analysis on the total population of Croatian patients with advanced/metastatic uterine cancer where the tumor CGP was performed during 2020. CGP of the tumor tissue of 32 patients revealed clinically relevant genomic alterations (CRGA) in 27 patients (84%) with a median of 3 (IQR 1-4) CRGA per patient. The most common CRGAs were those of phosphatide-inositol-3 kinases (PIK3) in 22 patients (69%), with 13/22 (59%) of those patients harboring PIK3CA mutation. The next most common CGRAs were ARID1A and PTEN mutations in 13 (41%) and 11 (34%) patients, respectively. Microsatellite status was determined as stable in 21 patients (66%) and highly unstable in 10 patients (31%). A high tumor mutational burden (≥10Muts/Mb) was reported in 12 patients (38%). CGP analysis reported some kind of targeted therapy for 28 patients (88%). CGP determined clinically relevant genomic alterations in the significant majority of patients with metastatic uterine cancer, defining it as a rich ground for further positioning and development of precision oncology.
Effects of Different Fermented Feeds on Production Performance, Cecal Microorganisms, and Intestinal Immunity of Laying Hens
Authors: Guo Lijuan , Lv Jing , Liu Yinglu , Ma Hui , Chen Bingxu , Hao Keyang , Feng Jia , Min Yuna
Published: September 2021

This experiment was conducted to investigate the effects of different compound probiotics on the performance, cecal microflora, and intestinal immunity of laying hens. A total of 270 Jing Fen No.6 (22-week-old) were randomly divided into 3 groups: basal diet (CON); basal diet supplemented with 6% fermented feed A by Bacillussubtilis,Lactobacillus, and Yeast (FA); and with 6% fermented feed B by C. butyricum and L. salivarius (FB). Phytic acid, trypsin inhibitor, β-glucan concentrations, and pH value in fermented feed were lower than the CON group (p < 0.05). The feed conversion ratio (FCR) in the experimental groups was decreased, while albumen height and Haugh unit were increased, compared with the CON group (p < 0.05). Fermented feed could upregulate the expression of the signal pathway (TLR4/MyD88/NF-κB) to inhibit mRNA expression of pro-inflammatory cytokines (p < 0.05). Fermented feed promoted the level of Romboutsia (in the FA group) Butyricicoccus (in the FB group), and other beneficial bacteria, and reduced opportunistic pathogens, such as Enterocooccus (p < 0.05). Spearman’s correlations showed that the above bacteria were closely related to albumen height and intestinal immunity. In summary, fermented feed can decrease the feed conversion ratio, and improve the performance and intestinal immunity of laying hens, which may be related to the improvement of the cecal microflora structure.
Effects of Induced Malocclusion on Vertebral Alignment in Rats: A Controlled Study by CBCTs
Authors: D’Attilio Michele , Cesaretti Gianfranco , Viganò Paolo , Apaza Alccayhuaman Karol Alí , Botticelli Daniele , Silva Erick Ricardo , Xavier Samuel Porfirio
Published: September 2021

This study aimed to evaluate with CBCTs the alteration of vertebral alignment over time of induced malocclusion in rats. Crown pads increasing the vertical dimension of 0.5 mm were applied on the upper molars at one randomly selected side of the maxilla in rats (premature contact side) while the opposite side was left untreated (control side). Four groups were organized, ten animals each. In groups A, B, and C, the crowns were applied at time 0 (t-0). In group A, the crowns were removed after 2 weeks (t-2w) and euthanized after two more weeks, while in groups B and C, the animals were euthanized after 2 and 4 weeks (t-4w), respectively. No premature contacts were applied in group D. CBCTs were taken at t-0 in all animals before applying the crowns, at t-2w in group A before removing the crowns, and in all groups before the euthanasia. The changes in the iliac crest angle (ICA) that formed between the superior external margin of the iliac crest and the vertebral spine were evaluated. In groups A and B, after 2 weeks, the changes in ICA were statistically significant at p = 0.028 and p = 0.042, respectively. In group C, and in the control group D, the changes of ICA were not statistically significant (p = 0.058 and p = 0.414, respectively). In conclusion, the incease in monolateral occlusion in the molar region yielded a rotation of the lumbo-sacral segment towards the same side of the occlusal bite-raising.
The Validation of a Non-Invasive Skin Sampling Device for Detecting Cetacean Poxvirus
Authors: Segura-Göthlin Simone , Fernández Antonio , Arbelo Manuel , Felipe-Jiménez Idaira , Colom-Rivero Ana , Almunia Javier , Sierra Eva
Published: September 2021

Poxvirus-like lesions are widely used as a potential health indicator in cetaceans, although for this application, corroboration of Poxvirus skin disease is imperative. Aiming to address skin biopsies intrusiveness, a preliminary investigation of a non-invasive skin sampling procedure to molecularly detect CePV-1 in 12 tattoo-like-lesions from two free-ranging stranded cetaceans in the Canary Islands was performed. Skin lesions were brushed with cytology cell samplers (CCSs) and placed into 1.5 mL microcentrifuge tubes with 1 mL of RNAlaterTM Stabilization Solution. For factual comparisons, DNA extractions from sloughed skin obtained with CCS and biopsies from the same lesions were accomplished with DNA Tissue Kit STM (QuickGene, Kurabo, Japan). Moreover, a second DNA extraction from sloughed skin with DNeasyTM Blood and Tissue Kit (Qiagen, Inc., Valencia, CA, USA) was performed to ascertain kit suitability for CCS. Molecular detection of CePV-1 was performed through a real-time PCR. As a result, a 91.7% and 83.3% rates of positivity were obtained with biopsies and CCS through Quickgene, respectively, compared to the rate of 100% using CCS with Qiagen. Accordingly, CCS is a reliable non-invasive sampling device to obtain sufficient genetic material to be analyzed for CePV-1 in tattoo-skin-lesions as well as for other purposes in cetaceans under human care.
Unanswered Questions and Hypotheses about Domestic Cat Behavior, Ecology, and the Cat–Human Relationship
Authors: Turner Dennis C.
Published: September 2021

After recent publication of several reviews covering research results from the last 35 years of domestic cat studies, a number of important unanswered questions and hypotheses have arisen that could interest active researchers, especially those beginning their academic careers. Some sections of this paper concern methodologies that have yielded new insights and could provide more in the future; other sections concern findings and interpretations of those that need further testing. First, hypotheses arise from combining subjective (or psychological) assessments of cat and human personality traits and observational (ethological) studies of cat–human interactions: e.g., do owners with high attachment to their cats interact differently with them than owners with low attachment levels? New analytical methods of dyadic interaction observations open the door for testing further hypotheses. In particular, the Theme® (Noldus bv, NL) program could be used to determine if there are differences between cat breeds in interaction patterns with people, which is not only of interest to owners but also therapists employing cats in their practices. Cat breed differences have been found using subjective ratings, but these need to be corroborated by direct observational data from the home setting and/or non-invasive colony observations, since ratings based on anthropomorphic projections might not be reliable. This should be done before searching for the genetic basis of such differences. Reliable information on breed differences is also needed before prescribing certain breeds for animal-assisted interventions. A model has predicted that the degree of socialization as a kitten affects cats’ responses to positive and negative experiences with unfamiliar humans and their formation of feline–human relationships later on. This needs to be tested in an ethically approved manner on cats of known socialization status and has enormous consequences for cat adoptions from animal shelters. Observations of human–cat interactions have yielded many correlations, which can be tested by non-invasive manipulations of human behavior in the home setting. Examples of these will be given and are of general interest to the cat-owning public. A review of first findings on social cognition in cats has resulted in further unanswered questions and hypotheses. Finally, two aspects of domestic cat ecology will be considered (effects on wildlife and space utilization), which are of great interest to the public and conservationists alike.
Impact of Silibinin A on Bioenergetics in PC12APPsw Cells and Mitochondrial Membrane Properties in Murine Brain Mitochondria
Authors: Esselun Carsten , Bruns Bastian , Hagl Stephanie , Grewal Rekha , Eckert Gunter P.
Published: September 2021

Age-related multifactorial diseases, such as the neurodegenerative Alzheimer’s disease (AD), still remain a challenge to today’s society. One mechanism associated with AD and aging in general is mitochondrial dysfunction (MD). Increasing MD is suggested to trigger other pathological processes commonly associated with neurodegenerative diseases. Silibinin A (SIL) is the main bioactive compound of the Silymarin extract from the Mediterranean plant Silybum marianum (L.) (GAERTN/Compositae). It is readily available as a herbal drug and well established in the treatment of liver diseases as a potent radical scavenger reducing lipid peroxidation and stabilize membrane properties. Recent data suggest that SIL might also act on neurological changes related to MD. PC12APPsw cells produce low levels of human Aβ and thus act as a cellular model of early AD showing changed mitochondrial function. We investigated whether SIL could affect mitochondrial function by measuring ATP, MMP, as well as respiration, mitochondrial mass, cellular ROS and lactate/pyruvate concentrations. Furthermore, we investigated its effects on the mitochondrial membrane parameters of swelling and fluidity in mitochondria isolated from the brains of mice. In PC12APPsw cells, SIL exhibits strong protective effects by rescuing MMP and ATP levels from SNP-induced mitochondrial damage and improving basal ATP levels. However, SIL did not affect mitochondrial respiration and mitochondrial content. SIL significantly reduced cellular ROS and pyruvate concentrations. Incubation of murine brain mitochondria with SIL significantly reduces Ca2+ induced swelling and improves membrane fluidity. Although OXPHOS activity was unaffected at this early stage of a developing mitochondrial dysfunction, SIL showed protective effects on MMP, ATP- after SNP-insult and ROS-levels in APPsw-transfected PC12 cells. Results from experiments with isolated mitochondria imply that positive effects possibly result from an interaction of SIL with mitochondrial membranes and/or its antioxidant activity. Thus, SIL might be a promising compound to improve cellular health when changes to mitochondrial function occur.
Case-Based Reasoning with an Artificial Neural Network for Decision Support in Situations at Complex Technological Objects of Urban Infrastructure
Authors: Glukhikh Igor , Glukhikh Dmitry
Published: September 2021

The article considers the tasks of intellectual support for decision support in relation to a complex technological object. The relevance is determined by a high level of responsibility, together with a variety of possible situations at a complex technological facility. The authors consider case-based reasoning (CBR) as a method for decision support. For a complex technological object, the problem defined is the uniqueness of the situations, which is determined by a variety of elements and the possible environmental influence. This problem complicates the implementation of CBR, especially the stages of comparing situations and a further selection of the most similar situation from the database. As a solution to this problem, the authors consider the use of neural networks. The work examines two neural network architectures. The first part of the research presents a neural network model that builds upon the multilayer perceptron. The second part considers the “Comparator-Adder” architecture. Experiments have shown that the proposed neural network architecture “Comparator-Adder” showed higher accuracy than the multilayer perceptron for the considered tasks of comparing situations. The results have a high level of generalization and can be used for decision support in various subject areas and systems where complex technological objects arise.
Global Clear-Sky Aerosol Speciated Direct Radiative Effects over 40 Years (1980–2019)
Authors: Korras-Carraca Marios-Bruno , Gkikas Antonis , Matsoukas Christos , Hatzianastassiou Nikolaos
Published: September 2021

We assess the 40-year climatological clear-sky global direct radiative effect (DRE) of five main aerosol types using the MERRA-2 reanalysis and a spectral radiative transfer model (FORTH). The study takes advantage of aerosol-speciated, spectrally and vertically resolved optical properties over the period 1980–2019, to accurately determine the aerosol DREs, emphasizing the attribution of the total DREs to each aerosol type. The results show that aerosols radiatively cool the Earth’s surface and heat its atmosphere by 7.56 and 2.35 Wm−2, respectively, overall cooling the planet by 5.21 Wm−2, partly counterbalancing the anthropogenic greenhouse global warming during 1980–2019. These DRE values differ significantly in terms of magnitude, and even sign, among the aerosol types (sulfate and black carbon aerosols cool and heat the planet by 1.88 and 0.19 Wm−2, respectively), the hemispheres (larger NH than SH values), the surface cover type (larger land than ocean values) or the seasons (larger values in local spring and summer), while considerable inter-decadal changes are evident. These DRE differences are even larger by up to an order of magnitude on a regional scale, highlighting the important role of the aerosol direct radiative effect for local and global climate.