bluuebunny/crossref_metadata_2025 huggingface dataset:
Elevation Accuracy of Forest Road Maps Derived from Aerial Imaging, Airborne Laser Scanning and Mobile Laser Scanning Data
Authors: Kardoš Miroslav , Sačkov Ivan , Tomaštík Julián , Basista Izabela , Borowski Łukasz , Ferenčík Michal
Published: May 2024

Forest road maps are a fundamental source of information for the sustainable management, protection, and public utilization of forests. However, the precision of these maps is crucial to their use. In this context, we assessed and compared the elevation accuracy of terrain on three forest road surfaces (i.e., asphalt, concrete, and stone), which were derived based on data from three remote sensing technologies (i.e., aerial imaging, airborne laser scanning, and mobile laser scanning) using five geospatial techniques (i.e., inverse distance; natural neighbor; and conversion by average, maximal, and minimal elevation value). Specifically, the elevation accuracy was assessed based on 700 points at which elevation was measured in the field, and these elevations were extracted from fifteen derived forest road maps with a resolution of 0.5 m. The highest precision was found on asphalt roads derived from mobile laser scanning data (RMSE from ±0.01 m to ±0.04 m) and airborne laser scanning data (RMSE from ±0.03 m to ±0.04 m). On the other hand, the lowest precision was found on all roads derived from aerial imaging data (RMSE from ±0.11 m to ±0.23 m). Furthermore, we found significant differences in elevation between the measured and derived terrains. However, the differences in elevation between specific techniques, such as inverse distance, natural neighbor, and conversion by average, were mostly random. Moreover, we found that airborne and mobile laser scanning technologies provided terrain on concrete and stone roads with random elevation differences. In these cases, it is possible to replace a specific technique or technology with one that is similar without significantly decreasing the elevation accuracy (α = 0.05).
Evaluation of Mechanical Wood Properties of Silver Birch (Betula pendula L. Roth.) of Half-Sib Genetic Families
Authors: Šilinskas Benas , Varnagirytė-Kabašinskienė Iveta , Beniušienė Lina , Aleinikovas Marius , Škėma Mindaugas , Baliuckas Virgilijus
Published: May 2024

Silver birch, a widely distributed deciduous tree native to Europe, is valued for its wood applications in construction, furniture making, and paper production. In Lithuania, silver birch ranks as the third most common forest-tree species, comprising 22% of the forested areas, and is an important species for tree breeding due to its potential and adaptability. This study was focused on assessing the mechanical properties of wood (sample and log hardness, wood density, dynamic modulus of elasticity (MOEdyn), static modulus of elasticity (MOE) and bending strength (MOR)) in silver birch (Betula pendula L. Roth.) trees from different half-sibling families. Two experimental plantations of the progenies of Lithuanian populations (half-sib families) of silver birch from different regions were analysed. From these plantations, four genetic families were selected for mechanical properties evaluation. The study findings revealed significant variability in various wood properties among different genetic families, although the static modulus of elasticity did not exhibit significant differences between the chosen genetic families. All measured wood properties decreased from the bottom to the top of the model trees. Wood hardness displayed a moderately negative correlation for wood density and weak correlations for MOE and MOR. Given the weak correlations between wood hardness and other wood mechanical properties, it is suggested that MOEdyn would be a more suitable trait for genetic studies.
Forest Therapy Research in Europe: A Scoping Review of the Scientific Literature
Authors: Mazzoleni Elena , Donelli Davide , Zabini Federica , Meneguzzo Francesco , Antonelli Michele
Published: May 2024

This scoping review aims to outline key discoveries, gaps in knowledge, and potential future directions in the field of forest therapy research in Europe. Clinical studies investigating the effects of forest therapy sessions in Europe were systematically screened through PubMed, Embase, Cochrane Library, the Directory of Open Access Journals (DOAJ), and Google Scholar (search date: January 2024). This review encompasses 26 selected studies, collectively engaging 2775 participants across various European countries, including Italy, Poland, Spain, Germany, Finland, Hungary, Sweden, Iceland, and Switzerland. These studies predominantly measured psychological outcomes such as mood states, affect, attention levels, subjective wellbeing, and quality of life. Additionally, physiological assessments were conducted, covering vital signs, salivary cortisol, body composition, and blood count. Notably, most of the investigation sites were covered by evergreen forests. Forest therapy practices in Europe demonstrated significant benefits in psychological aspects, including relaxation, mood enhancement, and improved wellbeing, accompanied by physiological improvements, particularly in asthma patients. While a fair-to-good methodological quality was identified in randomized controlled trials, the acknowledged weaknesses point towards a need for more rigorous and standardized research approaches. In conclusion, this comprehensive scoping review provides valuable insights into the current landscape of forest therapy research in Europe, highlighting its potential to positively impact both physical and mental wellbeing. The findings underscore the need for further research addressing methodological limitations and exploring gaps in understanding, thereby contributing to the continued advancement of forest therapy as a holistic wellbeing intervention within the European context.
A Review: Tree Species Classification Based on Remote Sensing Data and Classic Deep Learning-Based Methods
Authors: Zhong Lihui , Dai Zhengquan , Fang Panfei , Cao Yong , Wang Leiguang
Published: May 2024

Timely and accurate information on tree species is of great importance for the sustainable management of natural resources, forest inventory, biodiversity detection, and carbon stock calculation. The advancement of remote sensing technology and artificial intelligence has facilitated the acquisition and analysis of remote sensing data, resulting in more precise and effective classification of tree species. A review of the remote sensing data and deep learning tree species classification methods is lacking in its analysis of unimodal and multimodal remote sensing data and classification methods in this field. To address this gap, we search for major trends in remote sensing data and tree species classification methods, provide a detailed overview of classic deep learning-based methods for tree species classification, and discuss some limitations of tree species classification.
A Study of the Emotional and Cognitive Effects of Long-Term Exposure to Nature Virtual Reality (VR) Videos on Mobile Terminals
Authors: Wang Xiaobo , Jin Ying , Li Xuebing , Song Yang , Pan Dongni
Published: May 2024

Research Highlights: This study examined the emotional and cognitive health benefits of nature in comparison with working memory training. It considered the long-term effects, the application of mobile terminal technology, and routine-based approaches with the aim of integrating nature’s health benefits into people’s daily lives. Background and Objectives: Infectious diseases and aging may limit people’s activities indoors; the recovery effect of nature has been widely recognized, and terminal technology is developing rapidly. In this context, we want to explore the emotional and cognitive effects of viewing nature (VR) videos on mobile devices for a long time. Materials and Methods: The experiment employed a between-subjects design, with participants being randomly assigned to one of four groups: a forest VR video group, a water VR group, a working memory training group, and a control group. The participants watched the video three times a week for 20 min each for four weeks. The number of valid participants for compliance, preference, and willingness was 136, and the number of valid participants for the study of emotional and cognitive effects was 62. Brief Profile of Mood States (BPOMS) scales, running memory accuracy, shifting cost, etc., were used as indicators to reflect emotions and cognition. A repeated measures analysis of variance was performed on these indicators at four groups × two time points (pretest/post-test). Results: ① There were no significant differences in the participants’ adherence, preferences, and willingness to watch different natural videos and perform working memory training. ② Long-term home training (e.g., watching VR nature videos or working memory training) may have had a minimal effect on emotional responses to mobile terminals. However, home training may be more conducive to the stabilization of anger. ③ Watching forest VR videos had a positive effect on the updating function of the brain; watching water VR videos was beneficial for the shifting function and automatic processing speed; and working memory training had a positive effect on the updating and shifting functions. Conclusions: There were no significant differences in adherence, preference, willingness, and effects on emotion and cognition between long-term forest VR video viewing, water VR video viewing, and working memory training on mobile terminals. All three types of home training may be beneficial for the stabilization of emotion (especially anger), and all can have some positive effects on cognition.
Relative Expression of Genes Elicited by Clonostachys rosea in Pinus radiata Induces Systemic Resistance
Authors: Moraga-Suazo Priscila , Le-Feuvre Regis , Navarrete Dario , Sanfuentes Eugenio
Published: May 2024

Radiata pine is one of the most commonly planted tree species in Chile due to its fast growth and desirable wood and pulp properties. However, its productivity is hampered by several diseases. Pitch canker disease (PCC) caused by Fusarium circinatum, is considered the most damaging disease to the pine forest industry. Several control measures have been established, with biological control emerging as an environmentally friendly and effective way for F. circinatum control. Previous studies support the value of Clonostachys rosea in reducing PCC damage, with evidence suggesting a potential induced systemic resistance (ISR) triggered in radiata pines by this agent. Ten-month-old radiata pine plants were pre-treated with C. rosea on a substrate at 8 and 1 days before inoculation with F. circinatum on the stem tip, and expression levels were determined for DXS1, LOX, PAL, and PR3 genes 24 h later. Lesion length was 45% lower on plants pre-treated with C. rosea and infected with F. circinatum compared to non-pre-treated and infected plants. Additionally, LOX and PR3 were induced 23 and 62 times more, respectively, in comparison to untreated plants. Our results indicate that C. rosea causes an ISR response in pre-treated plants, significantly increasing the expression of resistance genes and reducing lesion length.
Bending Properties of Cold-Formed Thin-Walled Steel/Fast-Growing Timber Composite I-Beams
Authors: Chen Tianshu , Chen Zhihua , Liu Jiadi , Zhang Anling
Published: May 2024

A cold-formed, thin-walled steel/fast-growing timber composite system has recently been presented for low-rise buildings. It aims to increase the use of fast-growing wood as a green building material in structures, thus contributing to the transformation of traditional buildings. This study proposed a composite I-beam combined with fast-growing radiata pine and cold-formed thin-walled U-shaped steel. A four-point bending test was used to measure the bending properties of steel–timber composite I-beams under various connection methods. Based on experimental results, this study examined the specimen’s failure mechanism, mechanical properties, and strain development. In addition, a method for calculating flexural bearing capacity based on the superposition principle and transformed section method was suggested. It is evident from the results that fast-growing timber and cold-formed thin-walled steel can have significant composite effects. Different connecting methods significantly impact beams’ failure mode, stiffness, and bearing capacity. Furthermore, the theoretical method for calculating the flexural bearing capacity of composite beams differs from the test value by less than 10%. This paper’s research encourages the applications of fast-growing wood as light residential components, and it serves as a reference for the development, production, and engineering of steel–timber composite structural systems.
RMSRGAN: A Real Multispectral Imagery Super-Resolution Reconstruction for Enhancing Ginkgo Biloba Yield Prediction
Authors: Fan Kaixuan , Hu Min , Zhao Maocheng , Qi Liang , Xie Weijun , Zou Hongyan , Wu Bin , Zhao Shuaishuai , Wang Xiwei
Published: May 2024

Multispectral remote sensing data with abundant spectral information can be used to compute vegetation indices to improve the accuracy of Ginkgo biloba yield prediction. The limited spatial resolution of multispectral cameras restricts the detail capture over wide farmland, but super-resolution (SR) reconstruction methods can enhance image quality. However, most existing SR models have been trained on images processed from downsampled high-resolution (HR) images, making them less effective in reconstructing real low-resolution (LR) images. This study proposes a GAN-based super-resolution reconstruction method (RMSRGAN) for multispectral remote sensing images of Ginkgo biloba trees in real scenes. A U-Net-based network is employed instead of the traditional discriminator. Convolutional block attention modules (CBAMs) are incorporated into the Residual-in-Residual Dense Blocks (RRDBs) of the generator and the U-Net of the discriminator to preserve image details and texture features. An unmanned aerial vehicle (UAV) equipped with a multispectral camera was employed to capture field multispectral remote sensing images of Ginkgo biloba trees at different spatial resolutions. Four matching HR and LR datasets were created from these images to train RMSRGAN. The proposed model outperforms the traditional models by achieving superior results in both quantitative evaluation metrics (peak signal-to-noise ratio (PSNR) is 32.490, 31.085, 27.084, 26.819, and structural similarity index (SSIM) is 0.894, 0.881, 0.832, 0.818, respectively) and qualitative evaluation visualization. Furthermore, the efficiency of our proposed method was tested by generating individual vegetation indices (VIs) from images taken before and after reconstruction to predict the yield of Ginkgo biloba. The results show that the SR images exhibit better R2 and RMSE values than LR images. These findings show that RMSRGAN can improve the spatial resolution of real multispectral images, increasing the accuracy of Ginkgo biloba yield prediction and providing more effective and accurate data support for crop management.
Indoor Infrastructure Maintenance Framework Using Networked Sensors, Robots, and Augmented Reality Human Interface
Authors: Fath Alireza , Hanna Nicholas , Liu Yi , Tanch Scott , Xia Tian , Huston Dryver
Published: May 2024

Sensing and cognition by homeowners and technicians for home maintenance are prime examples of human–building interaction. Damage, decay, and pest infestation present signals that humans interpret and then act upon to remedy and mitigate. The maintenance cognition process has direct effects on sustainability and economic vitality, as well as the health and well-being of building occupants. While home maintenance practices date back to antiquity, they readily submit to augmentation and improvement with modern technologies. This paper describes the use of networked smart technologies embedded with machine learning (ML) and presented in electronic formats to better inform homeowners and occupants about safety and maintenance issues, as well as recommend courses of remedial action. The demonstrated technologies include robotic sensing in confined areas, LiDAR scans of structural shape and deformation, moisture and gas sensing, water leak detection, network embedded ML, and augmented reality interfaces with multi-user teaming capabilities. The sensor information passes through a private local dynamic network to processors with neural network pattern recognition capabilities to abstract the information, which then feeds to humans through augmented reality and conventional smart device interfaces. This networked sensor system serves as a testbed and demonstrator for home maintenance technologies, for what can be termed Home Maintenance 4.0.
Impact of Nanoparticle-Based TiO2 Surfaces on Norovirus Capsids and Genome Integrity
Authors: Raymond Philippe , St-Germain François , Paul Sylvianne , Chabot Denise , Deschênes Louise
Published: May 2024

Human noroviruses (HuNoVs) are among the main causes of acute gastroenteritis worldwide. HuNoVs can survive for several days up to weeks at room temperature in the environment, on food, and on food handling and processing surfaces. As a result, this could lead to viral spread through the ingestion of food in contact with contaminated surfaces. The development of stable surface materials with antiviral activity might be useful to reduce viral outbreaks. Metal-based compounds, including photoactivated titanium nanoparticles (TiO2 NPs), are known for their antiviral activity. In this study, we tested the impact of 2000 µg/mL TiO2 NPs, with or without UV activation, on HuNoV GII and murine norovirus. Their recovery rates were reduced by 99.6%. We also evaluated a new TiO2 NP-coating process on a polystyrene surface. This process provided a homogenous coated surface with TiO2 NPs ranging between 5 nm and 15 nm. Without photoactivation, this TiO2 NP-coated polystyrene surface reduced the recovery rates of intact HuNoV GII by more than 94%. When a capsid integrity treatment with PtCl4 or a longer reverse transcription polymerase chain detection approach was used to evaluate virus integrity following contact with the TiO2 NP-coated polystyrene, the HuNoV GII recovery yield reduction varied between 97 and 100%. These results support the hypothesis that TiO2 NP-coated surfaces have the potential to prevent viral transmission associated with contaminated food surfaces.
Assessing the Impact of Botanical Origins, Harvest Years, and Geographical Variability on the Physicochemical Quality of Serbian Honey
Authors: Tasić Aleksandra , Pezo Lato , Lončar Biljana , Pešić Mirjana B. , Tešić Živoslav , Kalaba Milica
Published: May 2024

This study summarized the physicochemical analysis of 609 honey samples originating from the Republic of Serbia. Variations among honey samples from different botanical origins, regions of collections, and harvest years were exposed to descriptive statistics and correlation analysis that differentiated honey samples. Furthermore, most of the observed physicochemical parameters (glucose, fructose, sucrose content, 5-hydroxymethylfurfural (5-HMF) levels, acidity, and electrical conductivity) varied significantly among different types of honey, years, and regions. At the same time, no noticeable difference was found in diastase activity, moisture content, and insoluble matter. Based on the obtained results, 22 honey samples could be considered adulterated, due to the irregular content of sucrose, 5-HMF, acidity, and diastase activity. In addition, 64 honey samples were suspected to be adulterated. Adulterated and non-compliant samples present a relatively low percentage (14.1%) of the total number of investigated samples. Consequently, a considerable number of honey samples met the required standards for honey quality. Overall, these findings provide insights into compositional and quality differences among various types of honey, aiding in understanding their characteristics and potential applications.
Determinants of Consumers’ Acceptance and Adoption of Novel Food in View of More Resilient and Sustainable Food Systems in the EU: A Systematic Literature Review
Authors: Laureati Monica , De Boni Annalisa , Saba Anna , Lamy Elsa , Minervini Fabio , Delgado Amélia M. , Sinesio Fiorella
Published: May 2024

This review article aims to provide an up-to-date overview of the main determinants of consumers’ acceptance of novel foods (new foods and ingredients) in the EU with emphasis on product’s intrinsic properties (sensory characteristics) and individual factors (socio-demographics, perceptive, psychological) by adopting a systematic approach following the PRISMA methodology. Case studies on terrestrial (i.e., insects, cultured meat and other animal origin products, plant-based food including mushrooms, plant-based analogues, pulses, and cereals) and aquatic systems (i.e., algae and jellyfish) are included focusing on age-related and cross-national differences in consumer acceptance of novel foods and ingredients. General trends have emerged that are common to all the novel foods analysed, regardless of their aquatic or terrestrial origin. Aspects such as food neophobia, unfamiliarity, and poor knowledge of the product are important barriers to the consumption of novel foods, while healthiness and environmental sustainability perception are drivers of acceptance. Sensory properties are challenging for more familiar ingredients such as plant-based food (e.g., novel food made by pulses, mushrooms, cereals and pseudocereals). Results are discussed in terms of feasibility of introducing these products in the EU food systems highlighting strategies that can encourage the use of new ingredients or novel foods.
Effect of Pod Storage and Drying Temperature on Fermentation Dynamics and Final Bean Quality of Cacao Nacional in Ecuador
Authors: Streule Stefanie , Freimüller Leischtfeld Susette , Chatelain Karin , Miescher Schwenninger Susanne
Published: May 2024

The impact of pod storage (PS) and two drying temperatures of fermented cocoa beans was investigated in Ecuador. Therefore, four variations were simultaneously carried out three times at two locations, independently: 0, 3, and 5 days of PS, dried at 60 °C and 0 days of PS, dried at 80 °C. Pod weight during storage, pulp content, pH, temperature, microbial counts, total free amino acids, protein profiles, sugars, organic acids, cut-test, fermentation index, and sensory profiles were analyzed. Minor differences in fermentation dynamics and bean quality were found between variations with and without PS. A rather accelerated fermentation with pod-stored beans was observed (e.g., faster color change, slightly lower pH in cotyledon after 48 h), along with a significantly higher maximal temperature during 24–42 h (43.1 ± 3.2 °C compared to 39.2 ± 2.0 °C without PS). More well-fermented beans were reached with PS (52.3 ± 22.6%) than without (62.7 ± 9.2%). Differences during fermentation were observed between the locations (e.g., pH, acids, sugars), but sensory evaluation indicated that the impact of location was mitigated with PS. Drying at 80 °C showed no adverse effects, as evidenced by the results of the cut-test and fermentation index. However, sensory evaluations revealed significant differences between 80 °C and 60 °C, with the former exhibiting more bitter and astringent cocoa liquor.
Soluble Dietary Fiber from Citrus unshiu Peel Promotes Antioxidant Activity in Oxidative Stress Mice and Regulates Intestinal Microecology
Authors: Fu Mengqi , Gao Xin , Xie Zuorui , Xia Chenlan , Gu Qing , Li Ping
Published: May 2024

Aging is characterized by the progressive degeneration of bodily tissues and decline in physiological functions, a process that may be exacerbated by imbalances in intestinal flora. Soluble dietary fiber (PSDF) from Citrus unshiu peel has demonstrated strong free radical scavenging ability to regulate intestinal flora in vitro. However, further evidence is required to ascertain the effectiveness of PSDF in vivo. In our study, 8-week-old mice were artificially aged through subcutaneous injections of a 200 mg/kg/d D-galactose solution for 42 days, followed by a 28-day dietary intervention with varying doses of PSDF, insoluble dietary fiber (PIDF), and vitamin C. After the intervention, we observed a significant mitigation of D-galactose-induced oxidative stress, as evident by weight normalization and reduced oxidative damage. 16S rRNA gene sequencing revealed that PSDF significantly altered the composition of intestinal flora, increasing Firmicutes and reducing Bacteroidota percentages, while also enriching colonic short-chain fatty acids (SCFAs). Spearman correlation analysis further identified a positive correlation between Firmicutes and isovaleric acid, and negative correlations between Muribaculaceae and acetic acid, and between Lachnospiraceae_NK4A136_group and caproic acid. These findings support the potential of Citrus PSDF to alleviate oxidative stress.
Investigating the Nutritional and Functional Properties of Protaetia brevitarsis Larvae and Isolated Soy Protein Mixtures as Alternative Protein Sources
Authors: Cho Eun-Chae , Ahn Surin , Hwang Hyo-Jeong , Shin Kyung-Ok , Kim Suwan , Choi Yean-Jung
Published: May 2024

The growing demand for sustainable and alternative protein sources has spurred interest in insect-based and plant-based proteins. Protaetia brevitarsis (PB) larvae and isolated soy protein (ISP) are notable in this regard, offering potential health benefits and nutritional enhancements. We assessed the feasibility of PB larvae and ISP mixtures as alternative food ingredients. Methods included the optimized purification and freeze-drying of PB larvae, extraction and refinement of legume proteins, physicochemical and antioxidant capacity evaluations, DPPH radical scavenging activity measurement, total phenolic and flavonoids content quantification, general component analysis, amino acid profiling using HPLC, fatty acid profiling through gas chromatography, and mineral content analysis using inductively coupled plasma spectrometry. The study found that certain PB:ISP ratios, particularly a 7:3 ratio, significantly improved the blend’s antioxidant capacity, as evidenced by DPPH scavenging activity. This ratio also impacted the nutritional profile by altering the mixture’s general components, with a notable increase in moisture, crude protein, and fiber and a decrease in crude fat and ash. Amino acid analysis revealed a balanced presence of essential and non-essential amino acids. The fatty acid profile was rich in unsaturated fatty acids, especially in certain ratios. Mineral analysis showed a complex interplay between PB larvae and ISP, with some minerals decreasing and others increasing in the blend. PB larvae and ISP mixtures have significant potential as alternative protein sources, offering a diversified nutritional profile and enhanced antioxidant properties. The 7:3 ratio of PB larvae to ISP has been shown to be particularly effective, suggesting that this ratio may offer an optimal balance for enhancing the overall nutritional quality of the mixture. This study sets the stage for future research to further explore and optimize the potential of these mixtures for human consumption while considering the challenges of consumer acceptance and long-term safety.
Combustion Diagnosis in a Spark-Ignition Engine Fueled with Syngas at Different CO/H2 and Diluent Ratios
Authors: Martinez-Boggio Santiago , Lacava Pedro Teixeira , de Carvalho Felipe Solferini , Curto-Risso Pedro
Published: May 2024

The gasification of residues into syngas offers a versatile gaseous fuel that can be used to produce heat and power in various applications. However, the application of syngas in engines presents several challenges due to the changes in its composition. Such variations can significantly alter the optimal operational conditions of the engines that are fueled with syngas, resulting in combustion instability, high engine variability, and misfires. In this context, this work presents an experimental investigation conducted on a port-fuel injection spark-ignition optical research engine using three different syngas mixtures, with a particular focus on the effects of CO/H2 and diluent ratios. A comparative analysis is made against methane, considered as the baseline fuel. The in-cylinder pressure and related parameters are examined as indicators of combustion behavior. Additionally, 2D cycle-resolved digital visualization is employed to trace flame front propagation. Custom image processing techniques are applied to estimate flame speed, displacement, and morphological parameters. The engine runs at a constant speed (900 rpm) and with full throttle like stationary engine applications. The excess air–fuel ratios vary from 1.0 to 1.4 by adjusting the injection time and the spark timing according to the maximum brake torque of the baseline fuel. A thermodynamic analysis revealed notable trends in in-cylinder pressure traces, indicative of differences in combustion evolution and peak pressures among the syngas mixtures and methane. Moreover, the study quantified parameters such as the mass fraction burned, combustion stability (COVIMEP), and fuel conversion efficiency. The analysis provided insights into flame morphology, propagation speed, and distortion under varying conditions, shedding light on the influence of fuel composition and air dilution. Overall, the results contribute to advancing the understanding of syngas combustion behavior in SI engines and hold implications for optimizing engine performance and developing numerical models.
Effect of Concomitant Use of Polaprezinc and Vonoprazan-Based Triple Therapy for Helicobacter pylori Eradication
Authors: Suzuki Yuto , Katayama Yasumi , Fujimoto Yo , Toyoda Koji , Takahashi Morio , Tamano Masaya
Published: May 2024

Background: Vonoprazan-based triple therapy has recently been reported as being more effective than proton pump inhibitors for the eradication of Helicobacter pylori (H. pylori), but it is apparent that the eradication rate could be further improved. Methods: We investigated the effect of the concomitant use of polaprezinc, a therapeutic agent for gastric ulcers, and vonoprazan-based seven-day triple therapy in patients with gastric ulcers compared to standard vonoprazan-based seven-day triple therapy in patients with atrophic gastritis. The regimen for the treatment of atrophic gastritis contained vonoprazan 20 mg, amoxicillin 750 mg, and clarithromycin 200 mg b.d. (VAC group) for seven days; and that for gastric ulcers contained VAC and polaprezinc 75 mg b.d. (VACP group) for seven days. Results: Between October 2021 and January 2023, 201 subjects were examined (VAC group, n = 165; VACP group, n = 36). In per-protocol (PP) analysis, the eradication rate was significantly higher in the VACP group (100%) than in the VAC group (88.2%) (p = 0.025). In patients with severe atrophic gastritis, eradication rates were significantly higher in the VACP group (100%) than in the VAC group (84.4%) in PP analysis. (p = 0.024). Conclusions: The concomitant use of polaprezinc and standard vonoprazan-based first-line eradication therapy is effective for H. pylori.
The MnO2/GelMA Composite Hydrogels Improve the ROS Microenvironment of Annulus Fibrosus Cells by Promoting the Antioxidant and Autophagy through the SIRT1/NRF2 Pathway
Authors: Xu Bohan , Huang Mingxuan , Li Jiaying , Meng Qingchen , Hu Jie , Chen Qianglong , He Hui , Jiang Hao , Han Fengxuan , Meng Bin , Liang Ting
Published: May 2024

Intervertebral disc degeneration (IVDD) is a worldwide disease that causes low back pain and reduces quality of life. Biotherapeutic strategies based on tissue engineering alternatives, such as intervertebral disc scaffolds, supplemented by drug-targeted therapy have brought new hope for IVDD. In this study, to explore the role and mechanism of MnO2/GelMA composite hydrogels in alleviating IVDD, we prepared composite hydrogels with MnO2 and methacrylate gelatin (GelMA) and characterized them using compression testing and transmission electron microscopy (TEM). Annulus fibrosus cells (AFCs) were cultured in the composite hydrogels to verify biocompatibility by live/dead and cytoskeleton staining. Cell viability assays and a reactive oxygen species (ROS) probe were used to analyze the protective effect of the composite hydrogels under oxidative damage. To explore the mechanism of improving the microenvironment, we detected the expression levels of antioxidant and autophagy-related genes and proteins by qPCR and Western blotting. We found that the MnO2/GelMA composite hydrogels exhibited excellent biocompatibility and a porous structure, which promoted cell proliferation. The addition of MnO2 nanoparticles to GelMA cleared ROS in AFCs and induced the expression of antioxidant and cellular autophagy through the common SIRT1/NRF2 pathway. Therefore, the MnO2/GelMA composite hydrogels, which can improve the disc microenvironment through scavenging intracellular ROS and resisting oxidative damage, have great application prospects in the treatment of IVDD.
Identification of Breed-Specific SNPs of Danish Large White Pig in Comparison with Four Chinese Local Pig Breed Genomes
Authors: Wu Xudong , Xiang Decai , Zhang Wei , Ma Yu , Zhao Guiying , Yin Zongjun
Published: May 2024

Genetic variation facilitates the evolution, environmental adaptability, and biodiversity of organisms. Danish Large White (LW) pigs have more desirable phenotypes compared with local Chinese pigs, which have difficulty adapting to the modern swine industry. However, the genome-wide mutational differences between these pig breeds are yet to be evaluated. Therefore, this study aimed to evaluate genomic variation and identify breed-specific SNPs in Danish LW pigs. Here, 43 LW, 15 Diqing Tibetan (DQZ), and 15 Diannan small-ear (DN) pigs whose genomes were re-sequenced with 5× depth were selected. This was followed by a conjoined analysis of our previous resequencing data of 24 Anqing six-end white (AQ) and six Asian wild (SS) pigs. In total, 39,158,378 SNPs and 13,143,989 insertion–deletions were obtained in all breeds. The variation number of LW pigs was the lowest, with 287,194 breed-specific and 1289 non-synonymous SNPs compared with Chinese breeds. Functional analysis of the breed-specific non-synonymous SNPs indicated that these mutations were mainly associated with the reproductive performance, feed intake, and feed conversion ratio of LW pigs. These findings provide a theoretical basis for genetic improvements in the Chinese swine industry.
The Effect of Short- and Long-Term Cryopreservation on Chicken Primordial Germ Cells
Authors: Ibrahim Mariam , Grochowska Ewa , Lázár Bence , Várkonyi Eszter , Bednarczyk Marek , Stadnicka Katarzyna
Published: May 2024

Primordial germ cells (PGCs) are the precursors of functional gametes and the only cell type capable of transmitting genetic and epigenetic information from generation to generation. These cells offer valuable starting material for cell-based genetic engineering and genetic preservation, as well as epigenetic studies. While chicken PGCs have demonstrated resilience in maintaining their germness characteristics during both culturing and cryopreservation, their handling remains a complex challenge requiring further refinement. Herein, the study aimed to compare the effects of different conditions (freezing-thawing and in vitro cultivation) on the expression of PGC-specific marker genes. Embryonic blood containing circulating PGCs was isolated from purebred Green-legged Partridgelike chicken embryos at 14–16 Hamburger–Hamilton (HH) embryonic development stage. The blood was pooled separately for males and females following sex determination. The conditions applied to the blood containing PGCs were as follows: (1) fresh isolation; (2) cryopreservation for a short term (2 days); and (3) in vitro culture (3 months) with long-term cryopreservation of purified PGCs (~2 years). To characterize PGCs, RNA isolation was carried out, followed by quantitative reverse transcription polymerase chain reaction (RT-qPCR) to assess the expression levels of specific germ cell markers (SSEA1, CVH, and DAZL), as well as pluripotency markers (OCT4 and NANOG). The investigated genes exhibited consistent expression among PGCs maintained under diverse conditions, with no discernible differences observed between males and females. Notably, the analyzed markers demonstrated higher expression levels in PGCs when subjected to freezing than in their freshly isolated counterparts.
The Role of Homogeneous Waiting Group Criteria in Patient Referrals: Views of General Practitioners and Specialists in South Tyrol, Italy
Authors: Piccoliori Giuliano , Wiedermann Christian J. , Barbieri Verena , Engl Adolf
Published: May 2024

Homogeneous waiting group (HWG) criteria are central to the patient referral process, guiding primary care physicians and hospitalists in directing patient care to specialists. This cross-sectional observational study, conducted in South Tyrol, Italy, in 2023, aimed to assess the implementation and impact of HWG criteria on healthcare from the perspective of general practitioners and hospital physicians. A questionnaire was developed to gain knowledge about referral practices as perceived by general practitioners and specialists. The survey included 313 participants (82 general practitioners and 231 hospital physicians) and was designed to capture a range of factors influencing the application of HWG criteria, including communication and collaboration practices. The results showed moderate levels of familiarity with HWG criteria and opinions about the need for criteria refinement among hospitalists, indicating that further education and refinement of these criteria are warranted. Both general practitioners and hospital physicians expressed dissatisfaction with the current specialist referral system, highlighting the significant gaps in effective communication and collaboration. The survey also demonstrated the influence of patient demands and waiting times on referral practices, and the need for streamlined and accessible specialist care. This study highlights the need for improvement and adaptation of HWG criteria to better meet the needs of healthcare providers and patients in South Tyrol. By addressing the identified gaps in communication, collaboration, and education related to the HWG system, the efficiency, effectiveness, and patient-centeredness of the referral process can be improved, ultimately leading to better health outcomes.
The Collaboration of Private Hospitals with the Public Health Service: The Case of La Rioja, Spain (1986–2019)
Authors: Jiménez-Buñuales María Teresa , León-Sanz Pilar , González-Diego Paulino , González-Menorca Leonor
Published: May 2024

In Spain, the public National Health Service provides care to Spaniards and other residents and is tailored for a decentralized state of autonomies. Each Autonomous Community has legislative capacity in its organization and management. We study the case of the collaboration between private hospitals and the public health service in La Rioja, an Autonomous Community of Spain located in the North of the Iberian Peninsula, due to the importance that this relationship has in health systems, in general. We applied the case study method as a methodological tool in a long-term local study. The interpretation was carried out within a national context, which allows us to understand its meaning and the historical keys to hospital development in this region. Primary sources have been reviewed (mainly reports, catalogs, and censuses of hospitals from the Ministry of Health and the Government of La Rioja) and other secondary sources, located in archives, libraries, Institute of Rioja Studies, and Department of Health. The hospital system in La Rioja was characterized by a predominance of public beds compared with private ones, although there has been a growing trend in the number of private beds from 2013 onwards due to the incorporation of health and social care convalescent hospitals (two). La Rioja has been promoting public–private collaboration (seen as a strategic alliance) and focusing on agreements in the socio-health space, particularly using the management service agreement and the concession of work formulas. The development of the public health service in La Rioja, from 1986 to 2019, has been determined by a progressive lower dependence on specialized hospitals from other health services of neighboring Autonomous Communities and by a mixed public–private hospital system.
Systematic Review: HIV, Aging, and Housing—A North American Perspective, 2012–2023
Authors: Chaminuka Arthur S. , Prybutok Gayle , Prybutok Victor R. , Senn William D.
Published: May 2024

Advances in anti-retroviral therapy (ART) have decreased mortality rates and subsequently led to a rise in the number of HIV-positive people living longer. The housing experiences of this new population of interest—older adults (50 years and older) living with HIV—are under-researched. Understanding the housing experiences and unmet needs of older people with HIV can better provide comprehensive care services for them. This study’s systematic review evaluated the peer-reviewed literature reporting housing access/insecurity/assistance/options, housing impact, and unmet needs of older individuals living with HIV in North America from 2012 to 2023. Furthermore, Latent Semantic Analysis (LSA), a text-mining technique, and Singular Value Decomposition (SVD) for text clustering were utilized to examine unstructured data from the abstracts selected from the review. The goal was to allow for a better understanding of the relationships between terms in the articles and the identification of emerging public health key themes affecting older adults living with HIV. The results of text clustering yielded two clusters focusing on (1) improvements to housing and healthcare services access and policies and (2) unmet needs—social support, mental health, finance, food, and sexuality insecurities. Topic modeling demonstrated four topics, which we themed to represent (1) a holistic care approach; (2) insecurities—food, financial, sexuality, and other basic needs; (3) access to housing and treatment/care; and (4) homelessness and HIV-related health outcomes. Stable housing, food, and healthcare services access and availability are critical elements to incorporating comprehensive, holistic healthcare for older adults living with HIV. The aging population requires high-priority policies for accessible and equitable healthcare. Clinicians and policymakers should address individual barriers, adopt a patient-centered approach, increase doctor visits, provide competency training, ensure long-term follow-up, involve families, and improve patient education in care management, contributing to HIV/AIDS geriatric care models.
Holistic Sexual-Reproductive Healthcare Services and Needs for Queer Individuals: Healthcare Providers’ Perspectives
Authors: Seretlo Raikane James , Smuts Hanlie , Mokgatle Mathildah Mpata
Published: May 2024

There are ongoing debates and controversies about whether genderqueer individuals have specific sexual-reproductive healthcare services and needs (SRHSNs). This study intended to identify and explore queer-specific SRHSNs among healthcare providers (HCPs) in Gauteng Province, South Africa. This was an exploratory sequential mixed-methods study, and this article focuses on the qualitative findings of that investigation. Thirty-three HCPs were purposively sampled, and semi-structured one-on-one interviews were used to collect data between September and November 2023. The data were analyzed using thematic content analysis (TCA). The results of this study revealed nine main themes: a crucial need for inclusive healthcare facilities; a need for psychological, counseling, and therapeutic support in sexual and reproductive healthcare; access to sexual-reproductive education and integrating support; suggested reproductive health services for queer sexual wellness; improved accessibility and particular queer reproductive healthcare; optimizing services related to human immunodeficiency virus (HIV), pre-exposure prophylaxis (PrEP) access, and sexually transmitted illness (STI) treatment; genderqueer persons’ parenthood aspirations and empowerment; the safe availability of intimacy tools; and navigation transitions. A holistic and inclusive healthcare approach that fits psychological support, comprehensive sexual-reproductive education, and specialized services to accommodate the unique needs of queer individuals should be implemented and made easily accessible.
Molecular Identification of Genetic Diversity and Population Structure in Moroccan Male Date Palm (Phoenix dactylifera L.) Using Inter-Simple Sequence Repeat, Direct Amplification of Minisatellite DNA, and Simple Sequence Repeat Markers
Authors: Ibrahimi Maha , Brhadda Najiba , Ziri Rabea , Fokar Mohamed , Amghar Ilham , Gaboun Fatima , Habach Aicha , Meziani Reda , Elfadile Jamal , Abdelwahd Rabha , Diria Ghizlane
Published: May 2024

Understanding genetic diversity and population structure plays a vital role in the efficient use of available material in plant-breeding programs and in germplasm conservation strategies. In the present study, we aim to evaluate the genetic variations and population structure of male date palms from Morocco. The genetic diversity of 100 date palm (Phoenix dactylifera L.) genotypes was investigated using the performance of three types of molecular markers: inter-simple sequence repeats (ISSRs), direct amplification of minisatellite DNA (DAMD), and simple sequence repeats (SSRs). On the basis of their polymorphic information content (PIC) (ISSRs = 0.38; DAMD = 0.4; SSRs = 0.33), effective multiplex ratio (EMR) (ISSRs = 27.34; DAMD = 52.31; SSRs = 22.20), Resolving power Rp (ISSR = 13.81; DAMD = 28.73; SSR = 14.6), and marker index (MI) (ISSRs = 9.22; DAMD = 20.23; SSRs = 7.54) values, all markers used in our study are considered informative markers. Among them, DAMD markers demonstrated slightly higher informativeness compared to ISSR and SSR markers. A total of 216, 438, and 248 bands were, respectively, detected using ISSRs, DAMD, and SSRs, with 95%, 98% and 94% of polymorphism, respectively. The AMOVA results revealed considerable diversity within date palms. The PCOa results showed that males of Tinghir and Errachidia were regrouped into the same cluster, while males of Goulmima were separated into another group. A cluster and structure analysis separated the studied genotypes into three groups. One group comprises genotypes of males from Zagora with some female varieties scattered in this group. The second group includes male genotypes from Goulmima along with accessions of female and male varieties. The third group contains males of Errachidia, Tata and Tinghir populations. The cluster and structure analysis separated the studied genotypes according to their origin.