bluuebunny/crossref_metadata_2025 huggingface dataset:
Modeling Stray Capacitances of High-Voltage Capacitive Dividers for Conventional Measurement Setups
Authors: Mingotti Alessandro , Costa Federica , Peretto Lorenzo , Tinarelli Roberto , Mazza Paolo
Published: February 2021

Stray capacitances (SCs) are a serious issue in high-voltage (HV) applications. Their presence can alter the circuit or the operation of a device, resulting in wrong or even disastrous consequences. To this purpose, in this work, we describe the modeling of SCs in HV capacitive dividers. Such modeling does not rely on finite element analysis or complicated geometries; instead, it starts from an equivalent circuit of a conventional measurement setup described by the standard IEC 61869-11. Once the equivalent model including the SCs is found, closed expressions of the SCs are derived starting from the ratio error definition. Afterwards, they are validated in a simulation environment by implementing various circuit configurations. The results demonstrate the expressions applicability and effectiveness; hence, thanks to their simplicity, they can be implemented by system operators, researchers, and manufacturers avoiding the use of complicated methods and technologies.
Design and Analysis of a While-Drilling Energy-Harvesting Device Based on Piezoelectric Effect
Authors: Zheng Jun , Dou Bin , Li Zilong , Wu Tianyu , Tian Hong , Cui Guodong
Published: February 2021

A while-drilling energy harvesting device is designed in this paper to recovery energy along with the longitudinal vibration of the drill pipes, aiming to serve as a continuous power supply for downhole instruments during the drilling procedure. Radial size of the energy harvesting device is determined through the drilling engineering field experience and geological survey reports. A piezoelectric coupling model based on the selected piezoelectric material was established via COMSOL Multiphysics numerical simulation. The forced vibration was analyzed to determine the piezoelectric patch length range and their best installation positions. Modal analysis and frequency response research indicate that the natural frequency of the piezoelectric cantilever beam increased monotonously with the increase of the piezoelectric patch’ thickness before reaching an inflection point. Moreover, the simulation results imply that the peak voltage of the harvested energy varied in a regional manner with the increase of the piezoelectric patches. When the thickness of the piezoelectric patches was 1.2–1.4 mm, the designed device gained the best energy harvest performance with a peak voltage of 15–40 V. Works in this paper provide theoretical support and design reference for the application of the piezoelectric material in the drilling field.
Benchmarking Flexible Electric Loads Scheduling Algorithms
Authors: van der Linden Koos , Romero Natalia , de Weerdt Mathijs M.
Published: February 2021

Due to increasing numbers of intermittent and distributed generators in power systems, there is an increasing need for demand responses to maintain the balance between electricity generation and use at all times. For example, the electrification of transportation significantly adds to the amount of flexible electricity demand. Several methods have been developed to schedule such flexible energy consumption. However, an objective way of comparing these methods is lacking, especially when decisions are made based on incomplete information which is repeatedly updated. This paper presents a new benchmarking framework designed to bridge this gap. Surveys that classify flexibility planning algorithms were an input to define this benchmarking standard. The benchmarking framework can be used for different objectives and under diverse conditions faced by electricity production stakeholders interested in flexibility scheduling algorithms. Our contribution was implemented in a software toolbox providing a simulation environment that captures the evolution of look-ahead information, which enables comparing online planning and scheduling algorithms. This toolbox includes seven planning algorithms. This paper includes two case studies measuring the performances of these algorithms under uncertain market conditions. These case studies illustrate the importance of online decision making, the influence of data quality on the performance of the algorithms, the benefit of using robust and stochastic programming approaches, and the necessity of trustworthy benchmarking.
Performance Characteristics of A Micro Wind Turbine Integrated on A Noise Barrier
Authors: Chrysochoidis-Antsos Nikolaos , van Bussel Gerard J.W. , Bozelie Jan , Mertens Sander M. , van Wijk Ad J.M.
Published: February 2021

Micro wind turbines can be structurally integrated on top of the solid base of noise barriers near highways. A number of performance factors were assessed with holistic experiments in wind tunnel and in the field. The wind turbines underperformed when exposed in yawed flow conditions. The theoretical cosθ theories for yaw misalignment did not always predict power correctly. Inverter losses turned out to be crucial especially in standby mode. Combination of standby losses with yawed flow losses and low wind speed regime may even result in a net power consuming turbine. The micro wind turbine control system for maintaining optimal power production underperformed in the field when comparing tip speed ratios and performance coefficients with the values recorded in the wind tunnel. The turbine was idling between 20%–30% of time as it was assessed for sites with annual average wind speeds of three to five meters per second without any power production. Finally, the field test analysis showed that inadequate yaw response could potentially lead to 18% of the losses, the inverter related losses to 8%, and control related losses to 33%. The totalized loss led to a 48% efficiency drop when compared with the ideal power production measured before the inverter. Micro wind turbine’s performance has room for optimization for application in turbulent wind conditions on top of noise barriers.
Solution to Fault of Multi-Terminal DC Transmission Systems Based on High Temperature Superconducting DC Cables
Authors: Lee Chun-Kwon , Lee Gyu-Sub , Chang Seung-Jin
Published: February 2021

In this paper, we developed the small-signal state-space (SS) model of hybrid multi-terminal high-voltage direct-current (HVDC) systems and fault localization method in a failure situation. The multi-terminal HVDC (MTDC) system is composed of two wind farm side voltage-source converters (VSCs) and two grid side line-commutated converters (LCCs). To utilize relative advantages of the conventional line-commutated converter (LCC) and the voltage source converter (VSC) technologies, hybrid multi-terminal high-voltage direct-current (MTDC) technologies have been highlighted in recent years. For the models, grid side LCCs adopt distinct two control methods: master–slave control mode and voltage droop control mode. By utilizing root-locus analysis of the SS models for the hybrid MTDC system, we compare stability and responses of the target system according to control method. Furthermore, the proposed SS models are utilized in time-domain simulation to illustrate difference between master–slave control method and voltage droop control method. However, basic modeling method for hybrid MTDC system considering superconducting DC cables has not been proposed. In addition, when a failure occurs in MTDC system, conventional fault localization method cannot detect the fault location because the MTDC system is a complex form including a branch point. For coping with a failure situation, we propose a fault localization method for MTDC system including branch points. We model the MTDC system based on the actual experimental results and simulate a variety of failure scenarios. We propose the fault localization topology on a branch cable system using reflectometry method. Through the simulation results, we verify the performance of fault localization. In conclusion, guidelines to select control method in implementing hybrid MTDC systems for integrating offshore wind farms and to cope with failure method are provided in this paper.
Hybrid Washer Fluid for Primary Cementing
Authors: Kremieniewski Marcin
Published: February 2021

This article presents the results on the basis of which a new hybrid drilling washer fluid was designed. The use of fluid from such a drilling washer increases the mud-cake removal efficiency. Its operation is based on both chemical and mechanical removal of the mud cake. This article presents a group of agents and admixtures of various solid fractions, the appropriate selection of which enabled the design of a hybrid drilling washer fluid. The liquid has much better washing parameters than the drilling washers used so far. The tests were carried out in a drilling fluid flow simulator. A significant improvement in the scrubbing mud-cake removal efficiency resulted from the action of surfactants and fine-grained abrasive additives. Their proper concentration was also very important. The hybrid drilling washer fluid was designed on the basis of tests measuring the adhesion of the hardened cement slurry to the rock from which the previously produced mud was removed. In this way, the effectiveness of the washing liquids was determined. Upon analyzing the obtained results and correlating them with the reference samples, one can see a significant improvement in the efficiency of the removal of the drilling sediment by the hybrid drilling washer fluid. The hybrid drilling washer fluid is an innovative solution because it combines chemical and mechanical action in the removal of drilling fluid. Additionally, such a washing liquid has not been used so far.
The Effect of Face Mask Use on COVID-19 Models
Authors: Bai Fan , Brauer Fred
Published: February 2021

We begin with a simple model for the COVID-19 epidemic and add face mask usages and testing and quarantine of infectives. We estimate the effect on the reproduction number and discuss the question of whether the epidemic can be controlled by increased use of face masks.
Incidence and Severity of End-Splitting in Plantation-Grown Eucalyptus pellita F. Muell. in North Borneo
Authors: Espey Manuel , Md. Tahir Paridah , Lee Seng Hua , Muhammad Roseley Adlin Sabrina , Meder Roger
Published: February 2021

Eucalyptus pellita F. Muell. is currently the predominant tree species deployed for tree plantation establishment in some parts of Borneo, particularly Sabah state, Malaysia. Its low disease susceptibility, good growth and form, and desirable wood properties make E. pellita particularly suitable for plantation development in the wet tropical regions of south-east Asia. In spite the many positive traits, practical field observations indicate that the species is susceptible to end-splitting. There is some anecdotal evidence to suggest a genetic relationship with the incidence of end-split, although these observations were not statistically tested. This study evaluates the occurrence of end-splitting in five-year old plantation E. pellita in Sabah as affected by family and growth attributes. An existing progeny tree breeding trial, involving seeds that originated from Papua New Guinea, China, Vietnam, Australia, Sabah, and Sarawak with 106 families, was used to carry out the split assessment. Logs from the second thinning were cut into 2.2-m-long sections. The end splits were measured three days later; the incidence and severity of splitting were assessed at both the large and small ends of each log by using a designated split scoring system. The study shows that 99% of all log cross sections (per cut surface) experienced signs of splitting, with an average of 2.7 splits per cut surface. Overall, 54% of all splits were classed as ‘Three-Quarter Radial Splits’ while the occurrence of internal splits was negligible. Split severity showed a heritable and significant difference (h2 = 0.24 at p ≤ 0.05) among the family levels. Splitting was also significantly related to growth factors such as tree height and diameter at breast height (DBH). The findings suggest that end-splitting is caused by family–environment interactions. This offers the opportunity to improve the wood resource of E. pellita via breeding strategies.
Slight Increases in Salinity Improve Muscle Quality of Grass Carp (Ctenopharyngodon idellus)
Authors: Zhang Xi , Shen Zhiyuan , Qi Tianpeng , Xi Rujuan , Liang Xiao , Li Li , Tang Rong , Li Dapeng
Published: February 2021

Fish muscle quality is an important parameter in the aquaculture industry. In this study, we analyzed and compared the muscle quality of grass carp (Ctenopharyngodon idellus) cultured at salinities of 0‰, 3‰, and 6‰ (GC0, GC3, GC6). There was no significant difference in crude protein and crude fat content of muscle between GC0 and GC3. Crude fat was significantly lower in GC6 compared to the other groups. GC3 and GC6 had higher hydroxyproline content, which suggested that these groups had higher collagen content. GC3 and GC6 had higher contents of free amino acids and umami amino acids than GC0, but there was no significant difference in sweet or sour amino-acid content among groups. GC3 and GC6 had better texture properties, including hardness, gumminess, chewiness, resilience, and springiness, than GC0. GC3 had the highest water-holding capacity among the groups. As the salinity increased, the diameter of muscle fibers decreased and the sarcolemma showed a thickening trend. These results suggest that a slight increase in salinity (i.e., 3‰) can effectively improve the muscle quality of grass carp.
Encapsulation of Berberis vulgaris Anthocyanins into Nanoliposome Composed of Rapeseed Lecithin: A Comprehensive Study on Physicochemical Characteristics and Biocompatibility
Authors: Homayoonfal Mina , Mousavi Seyed Mohammad , Kiani Hossein , Askari Gholamreza , Desobry Stephane , Arab-Tehrany Elmira
Published: February 2021

In the present study, nanoliposomes composed of rapeseed lecithin were used for the encapsulation of anthocyanin compounds (AC). The nanoliposomes were prepared using hydration and ultrasound combined method, and the effect of AC concentration (4.5, 6.75, 9% w/w) on the characteristics of nanoliposomes including particle size, polydispersity index (PDI), zeta potential, and the encapsulation efficiency (EE) of nanoliposomes with and without AC were studied. The results suggested the fabricated nanoliposomes had a size range of 141–196 nm, negative zeta potential and narrow particle size distribution. Further, the samples containing 9% extract had the maximum EE (43%). The results showed elevation of AC concentration resulted in increased particle size, PDI, EE, and surface charge of nanoparticles. The presence of AC extract led to diminished membrane fluidity through the hydrophobic interactions with the hydrocarbon chain of fatty acids. TEM images suggested that the nanoliposomes were nearly spherical and the AC caused their improved sphericity. Further, in vitro biocompatibility tests for human mesenchymal (MSC) and fibroblast (FBL) cells indicated nanoparticles were not toxic. Specifically, the best formulations with the maximum compatibility and bioavailability for MSC and FBL cells were AC-loaded nanoliposomes with concentrations of 0.5 mL/mg and 10.3 mL/µg and, respectively.
Evaluation of Lipid Oxidation, Volatile Compounds and Vibrational Spectroscopy of Silver Carp (Hypophthalmichthys molitrix) during Ice Storage as Related to the Quality of Its Washed Mince
Authors: Kunyaboon Sasinee , Thumanu Kanjana , Park Jae W. , Khongla Chompoonuch , Yongsawatdigul Jirawat
Published: February 2021

Changes in the lipid oxidation of silver carp (Hypophthalmichthys molitrix) stored in ice for 14 days and that of its respective washed mince were evaluated. Total lipid, phospholipid, polyunsaturated fatty acid (PUFA) and monounsaturated fatty acid (MUFA) contents of the skin, belly flap and mince decreased as the storage time in ice increased. The washing process decreased the lipid contents but concentrated their phospholipid counterparts. The fish belly flap exhibited the highest thio-barbituric acid reactive substances (TBARS) value, while the mince had the lowest. 1-Hexanol, 1-octen-3-ol, and 1-hexanal were key volatile compounds detected in the belly flaps of fish stored for 7–14 days. Hexanal was the only major volatile compound found in washed mince prepared from fish stored for an extended period in ice, but in a much lower amount compared with that in the belly flap. FTIR (Fourier transform infra-red) spectra revealed a decrease in the number of cis double bonds, methylene groups and phosphate groups in lipids extracted from fish stored in ice for 7–14 days as compared with those extracted from fresh fish. Principle component analysis (PCA) revealed that the FT-Raman band at 1747 cm−1 could be a potential marker for tracking the degree of lipid oxidation in the belly flap of silver carp stored in ice. In addition, IR bands indicating phosphate group (925, 825 cm−1) in oil extracted from washed mince were correlated with the extent of the lipid oxidation of the raw material.
Fatty Acid Composition from Olive Oils of Portuguese Centenarian Trees Is Highly Dependent on Olive Cultivar and Crop Year
Authors: Rodrigues Nuno , Casal Susana , Pinho Teresa , Cruz Rebeca , Peres António M. , Baptista Paula , Pereira José Alberto
Published: February 2021

The high proportions of monounsaturated fatty acids (MUFA) represent one of the most important technological and nutritional features of olive oils. The present study details the fatty acid (FA) composition of autochthonous cultivars (Lentisca, Madural, Redondal, Rebolã, Verdeal, and Verdeal Transmontana) produced from centenarian trees during five crop years (2013–2017). Olive cultivar highly influenced the FA composition, namely, oleic acid (70.3% for Madural to 80.7% for Redondal) and palmitic acid (10.4% for Lentisca to 13.5% for Verdeal). Similarly, crop year significantly influenced the individual FA contents. Principal component analysis of FA data enabled the unsupervised classification by cultivar and, within each cultivar, by crop year. Furthermore, the levels of nine individual FAs, together with the polyunsaturated fatty acid contents, selected using the simulated annealing algorithm, allowed for their correct classification, on the basis of linear discriminant analysis, according to the olive cultivar, with an overall sensitivity of 92%, for leave-one-out cross-validation procedure. Globally, the cultivar effect superimposed that of crop year, showing that some cultivars, such as Redondal and Verdeal Transmontana, have consistently high and homogeneous proportions of MUFA, indicating that they are worth exploring in terms of future selection of cultivars that are able to produce olive oils with increased nutritional value and that are less prone to oxidation.
The Application of Essential Oil Vapors at the End of Vacuum Cooling of Fresh Culinary Herbs Promotes Aromatic Recovery
Authors: Martínez-Hernández Ginés Benito , Taboada-Rodríguez Amaury , Garre Alberto , Marín-Iniesta Fulgencio , López-Gómez Antonio
Published: February 2021

Aroma is an important quality parameter of fresh culinary herbs that may be highly affected after postharvest treatments. The innovative technology of vapor essential oil (EO) application under vacuum conditions may recover aroma lost during the postharvest processing of plant products like aromatic herbs. Hence, this study assessed the aroma recovery effect of vapor EOs applied during vacuum cooling on curly parsley and dill. The volatile organic compounds (VOCs) profiles of these aromatic herbs were studied by static headspace solid-phase microextraction (SPME), and the VOCs sorption kinetics onto the SPME stir-bar coating were modeled by the Baranyi model. At the pilot plant scale, the total VOCs contents of parsley and dill (whose extractability was increased by 10–20% after a single vacuum process) were enhanced by 4.5- and 2-fold, respectively, when vapor EOs were applied. In particular, 1,3,8-p-menthatriene and carvone (parsley) increased by 18.7- and 7.3-fold, respectively, while dill ether (the characteristic VOC of dill) augmented by 2.4-fold after vapor EOs were applied under vacuum conditions. The aroma recovery of culinary herbs was successfully validated at an industrial level in an installation developed by our group to apply vapor EOs within a vacuum cooling system, reaching total VOC recoveries of 4.9- and 2.3-fold in parsley and dill, respectively.
Sustainability Certification, a New Path of Value Creation in the Olive Oil Sector: The ITALIAN Case Study
Authors: Lombardo Luca , Farolfi Camilla , Capri Ettore
Published: February 2021

The Italian extra virgin olive oil supply chain has considerable potential for embarking on a path of sustainable development and evolution. In Italy, the great variety heritage and the different pedo-climatic characteristics result in local olive growing systems with different management techniques, producing extra virgin olive oils that are strictly entwined to the territory, with peculiar qualitative properties. Nevertheless, numerous criticalities have been traditionally eroding the competitiveness of Italian olive growing that could find in sustainability certifications, a lasting driver of value creation. Shared standardizations and certifications that include the three pillars of sustainability are therefore necessary for the development of the process.
Antioxidant Capacity and Phenolic and Sugar Profiles of Date Fruits Extracts from Six Different Algerian Cultivars as Influenced by Ripening Stages and Extraction Systems
Authors: Tassoult Malika , Kati Djamel Edine , Fernández-Prior María África , Bermúdez-Oria Alejandra , Fernandez-Bolanos Juan , Rodríguez-Gutiérrez Guillermo
Published: February 2021

The study investigated the phenols, sugar and the antioxidant capacities of date fruit extracts obtained by organic solvents and by hydrothermal treatment from six different Algerian cultivars at two ripening stages for the first time. The analyzed cultivars exhibited potent antioxidant properties (ferric reducing antioxidant power (FRAP), 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavenging capacities) and different phenols regardless of the solvents and the maturity stages. About 18 phenols were identified and quantified, mainly in the hydrothermal extracts. The earlier stages were characterized by high amounts of o-coumaric acid, cinnamic acid and luteolin, with a noticeable absence of quercetin. The tamr stage presented the highest sugar content (78.15–86.85 mg/100 mg dry weight (DW)) with an abundance of glucose. Galactose was present only in some cultivars from the kimri stage (tamjouhert). Uronic acids were mostly detected at the tamr stage (4.02–8.82 mg gallic acid equivalent/100 mg dried weight). The obtained results highlight the potential of using date fruit extracts as natural antioxidants, especially at industrial scales that tend use hydrothermal extraction.
Cirrhotic Cardiomyopathy
Authors: Longley Kieran , Tran Tuan , Ho Vincent
Published: February 2021

Cirrhotic cardiomyopathy (CCM), cardiac dysfunction in end-stage liver disease in the absence of prior heart disease, is an important clinical entity that contributes significantly to morbidity and mortality. The original definition for CCM, established in 2005 at the World Congress of Gastroenterology (WCG), was based upon known echocardiographic parameters to identify subclinical cardiac dysfunction in the absence of overt structural abnormalities. Subsequent advances in cardiovascular imaging and in particular myocardial deformation imaging have rendered the WCG criteria outdated. A number of investigations have explored other factors relevant to CCM, including serum markers, electrocardiography, and magnetic resonance imaging. CCM characteristics include a hyperdynamic circulatory state, impaired contractility, altered diastolic relaxation, and electrophysiological abnormalities, particularly QT interval prolongation. It is now known that cardiac dysfunction worsens with the progression of cirrhosis. Treatment for CCM has traditionally been limited to supportive efforts, but new pharmacological studies appear promising. Left ventricular diastolic dysfunction in CCM can be improved by targeted heart rate reduction. Ivabradine combined with carvedilol improves left ventricular diastolic dysfunction through targeted heart rate reduction, and this regimen can improve survival in patients with cirrhosis. Orthotopic liver transplantation also appears to improve CCM. Here, we canvass diagnostic challenges associated with CCM, introduce cardiac physiology principles and the application of echocardiographic techniques, and discuss the evidence behind therapeutic interventions in CCM.
Angiotensinogen Gene Missense Polymorphisms (rs699 and rs4762): The Association of End-Stage Renal Failure Risk with Type 2 Diabetes and Hypertension in Egyptians
Authors: El-Garawani Islam M. , Shaheen Eman M. , El-Seedi Hesham R. , Khalifa Shaden A. M. , Mersal Gaber A. M. , Emara Mahmoud M. , Kasemy Zeinab A.
Published: February 2021

Type 2 diabetes mellitus (T2DM) and hypertension are common chronic diseases mainly associated with the development and progression of end-stage renal disease (ESRD) leading to morbidity and mortality. Gene polymorphisms linked to the renin–angiotensin (AGT)–aldosterone system (RAAS) were broadly inspected in patients with diabetic nephropathy (DN) and hypertension. This study aimed to investigate the association of AGT gene polymorphisms (rs699 and rs4762) with ESRD in T2DM hypertensive Egyptian patients. Genotyping of rs699 and rs4762 was conducted using the tetra-primers amplification refractory mutation system (ARMS-PCR). The allelic distribution analysis was performed on 103 healthy control subjects, 97 non-ESRD patients, and 104 patients with ESRD. The allelic frequencies of AGT gene polymorphisms (rs4762 and rs699) in all study participants were assessed. For the non-ESRD group, the frequencies of the alleles of AGT-rs4762 (χ2 = 31.88, p < 0.001, OR = 5.17, CI 95%: 2.81–9.51) and AGT-rs699 (χ2 = 4.85, p = 0.027, OR = 1.56, CI 95%: 1.05–2.33) were significantly associated with the non-ESRD group. However, for the ESRD group, the T allele was significantly higher than that in the controls (χ2 = 24.97, p < 0.001, odds ratio (OR) = 4.35, CI 95%: 2.36–8.02). Moreover, AGT (rs699) genotypes showed no significant difference between the ESRD group and controls. In conclusion, AGT gene polymorphisms rs699 and rs4762 were associated with non-ESRD versus controls, without any significant risk observed in all patient groups. However, the AGT (rs4762) variant showed a significant risk in the ESRD group in comparison to controls in Egyptians.
The Amazing Acrobat: Yeast’s Histone H3K56 Juggles Several Important Roles While Maintaining Perfect Balance
Authors: Gershon Lihi , Kupiec Martin
Published: February 2021

Acetylation on lysine 56 of histone H3 of the yeast Saccharomyces cerevisiae has been implicated in many cellular processes that affect genome stability. Despite being the object of much research, the complete scope of the roles played by K56 acetylation is not fully understood even today. The acetylation is put in place at the S-phase of the cell cycle, in order to flag newly synthesized histones that are incorporated during DNA replication. The signal is removed by two redundant deacetylases, Hst3 and Hst4, at the entry to G2/M phase. Its crucial location, at the entry and exit points of the DNA into and out of the nucleosome, makes this a central modification, and dictates that if acetylation and deacetylation are not well concerted and executed in a timely fashion, severe genomic instability arises. In this review, we explore the wealth of information available on the many roles played by H3K56 acetylation and the deacetylases Hst3 and Hst4 in DNA replication and repair.
Two Italian Patients with ELOVL4-Related Neuro-Ichthyosis: Expanding the Genotypic and Phenotypic Spectrum and Ultrastructural Characterization
Authors: Diociaiuti Andrea , Martinelli Diego , Nicita Francesco , Cesario Claudia , Pisaneschi Elisa , Macchiaiolo Marina , Rossi Sabrina , Condorelli Angelo Giuseppe , Zambruno Giovanna , El Hachem May
Published: February 2021

Elongation of Very Long Chain Fatty Acid-4 (ELOVL4) is a fatty acid elongase responsible for very long-chain fatty acid biosynthesis in the brain, retina, and skin. Heterozygous mutations in ELOVL4 gene cause Stargardt-like macular dystrophy and spinocerebellar ataxia type-34, while different homozygous mutations have been associated with ichthyosis, spastic quadriplegia, and mental retardation syndrome in three kindred. We report the first two Italian children affected with neuro-ichthyosis due to the previously undescribed ELOVL4 homozygous frameshift variant c.435dupT (p.Ile146TyrfsTer29), and compound heterozygous variants c.208C>T (p.Arg70Ter) and c.487T>C (p.Cys163Arg), respectively. Both patients were born with collodion membrane followed by development of diffuse mild hyperkeratosis and scaling, localized erythema, and palmoplantar keratoderma. One infant displayed mild facial dysmorphism. They suffered from failure to thrive, and severe gastro-esophageal reflux with pulmonary aspiration. The patients presented axial hypotonia, hypertonia of limbs, and absent head control with poor eye contact from infancy. Visual evoked potentials showed markedly increased latency and poor morphological definition, indicative of alteration of the retro-retinal visual pathways in both patients. Ultrastructural skin examination revealed abnormalities of lamellar bodies with altered release in the epidermal granular and horny layer intracellular spaces. Our findings contribute to expanding the phenotypic and genotypic features of ELOVL4-related neuro-ichthyosis.
Geospatial Analysis of Rain Fields and Associated Environmental Conditions for Cyclones Eline and Hudah
Authors: Matyas Corene J. , VanSchoick Sarah
Published: February 2021

Tropical cyclones (TCs) that landfall over Madagascar and Mozambique can cause flooding that endangers lives. To better understand how environmental conditions affect the rain fields of these TCs, this study utilized spatial metrics to analyze two storms taking similar paths two months apart. Using a geographic information system, rain rates of 1 mm/h were extracted from a satellite-based dataset and contoured to define the rain field edge. Average extent of rainfall was measured for each quadrant and asymmetry was calculated along with rain field area, dispersion, closure, and solidity. Environmental conditions and storm intensity were analyzed every six hours. Results indicate that although both TCs intensified prior to first interaction with land, stronger vertical wind shear experienced by Eline was associated with higher asymmetry and dispersion. Additionally, rain fields were less solid although the center was mostly enclosed by rain. Storm shape was altered as both storms tracked over Madagascar, with Hudah recovering more quickly. Moisture increased for both storms and shear decreased for Eline, allowing it to become more centered and solid, and grow larger. Relationships between intensity, land interaction, and rain field shape support the results of previous research and demonstrate the global utility of these metrics.
Cervid Prion Protein Polymorphisms: Role in Chronic Wasting Disease Pathogenesis
Authors: Arifin Maria Immaculata , Hannaoui Samia , Chang Sheng Chun , Thapa Simrika , Schatzl Hermann M. , Gilch Sabine
Published: February 2021

Chronic wasting disease (CWD) is a prion disease found in both free-ranging and farmed cervids. Susceptibility of these animals to CWD is governed by various exogenous and endogenous factors. Past studies have demonstrated that polymorphisms within the prion protein (PrP) sequence itself affect an animal’s susceptibility to CWD. PrP polymorphisms can modulate CWD pathogenesis in two ways: the ability of the endogenous prion protein (PrPC) to convert into infectious prions (PrPSc) or it can give rise to novel prion strains. In vivo studies in susceptible cervids, complemented by studies in transgenic mice expressing the corresponding cervid PrP sequence, show that each polymorphism has distinct effects on both PrPC and PrPSc. It is not entirely clear how these polymorphisms are responsible for these effects, but in vitro studies suggest they play a role in modifying PrP epitopes crucial for PrPC to PrPSc conversion and determining PrPC stability. PrP polymorphisms are unique to one or two cervid species and most confer a certain degree of reduced susceptibility to CWD. However, to date, there are no reports of polymorphic cervid PrP alleles providing absolute resistance to CWD. Studies on polymorphisms have focused on those found in CWD-endemic areas, with the hope that understanding the role of an animal’s genetics in CWD can help to predict, contain, or prevent transmission of CWD.
Extracellular Vesicles in Allergic Rhinitis and Asthma and Laboratory Possibilities for Their Assessment
Authors: Demkow Urszula , Stelmaszczyk-Emmel Anna
Published: February 2021

Currently, extracellular vesicles (EVs) have been implicated in the etiopathogenesis of many diseases, including lung disorders, with the possibility of diagnostic and therapeutic applications. The analysis of EV in respiratory tract diseases faces many obstacles, including material collection from airways, standardization of isolation techniques, detection methods, the analysis of their content, etc. This review focuses on the role of extracellular vesicles in the pathogenesis of atopic respiratory diseases, especially asthma, with a special focus on their clinical applicability as a diagnostic tool. We also summarize available laboratory techniques that enable the detection of EVs in various biological materials, with particular emphasis on flow cytometry. The opportunities and limitations of detecting EV in bronchoalveolar lavage fluid (BALF) were also described.
Time-Resolved scRNA-Seq Tracks the Adaptation of a Sensitive MCL Cell Line to Ibrutinib Treatment
Authors: Fuhr Viktoria , Vafadarnejad Ehsan , Dietrich Oliver , Arampatzi Panagiota , Riedel Angela , Saliba Antoine-Emmanuel , Rosenwald Andreas , Rauert-Wunderlich Hilka
Published: February 2021

Since the approval of ibrutinib for relapsed/refractory mantle cell lymphoma (MCL), the treatment of this rare mature B-cell neoplasm has taken a great leap forward. Despite promising efficacy of the Bruton tyrosine kinase inhibitor, resistance arises inevitably and the underlying mechanisms remain to be elucidated. Here, we aimed to decipher the response of a sensitive MCL cell line treated with ibrutinib using time-resolved single-cell RNA sequencing. The analysis uncovered five subpopulations and their individual responses to the treatment. The effects on the B cell receptor pathway, cell cycle, surface antigen expression, and metabolism were revealed by the computational analysis and were validated by molecular biological methods. The observed upregulation of B cell receptor signaling, crosstalk with the microenvironment, upregulation of CD52, and metabolic reprogramming towards dependence on oxidative phosphorylation favor resistance to ibrutinib treatment. Targeting these cellular responses provide new therapy options in MCL.
Clade-Specific Plastid Inheritance Patterns Including Frequent Biparental Inheritance in Passiflora Interspecific Crosses
Authors: Shrestha Bikash , Gilbert Lawrence E. , Ruhlman Tracey A. , Jansen Robert K.
Published: February 2021

Plastid inheritance in angiosperms is presumed to be largely maternal, with the potential to inherit plastids biparentally estimated for about 20% of species. In Passiflora, maternal, paternal and biparental inheritance has been reported; however, these studies were limited in the number of crosses and progeny examined. To improve the understanding of plastid transmission in Passiflora, the progeny of 45 interspecific crosses were analyzed in the three subgenera: Passiflora, Decaloba and Astrophea. Plastid types were assessed following restriction digestion of PCR amplified plastid DNA in hybrid embryos, cotyledons and leaves at different developmental stages. Clade-specific patterns of inheritance were detected such that hybrid progeny from subgenera Passiflora and Astrophea predominantly inherited paternal plastids with occasional incidences of maternal inheritance, whereas subgenus Decaloba showed predominantly maternal and biparental inheritance. Biparental plastid inheritance was also detected in some hybrids from subgenus Passiflora. Heteroplasmy due to biparental inheritance was restricted to hybrid cotyledons and first leaves with a single parental plastid type detectable in mature plants. This indicates that in Passiflora, plastid retention at later stages of plant development may not reflect the plastid inheritance patterns in embryos. Passiflora exhibits diverse patterns of plastid inheritance, providing an excellent system to investigate underlying mechanisms in angiosperms.
Development of 18F-Labeled Radiotracers for PET Imaging of the Adenosine A2A Receptor: Synthesis, Radiolabeling and Preliminary Biological Evaluation
Authors: Lai Thu Hang , Schröder Susann , Toussaint Magali , Dukić-Stefanović Sladjana , Kranz Mathias , Ludwig Friedrich-Alexander , Fischer Steffen , Steinbach Jörg , Deuther-Conrad Winnie , Brust Peter , Moldovan Rareş-Petru
Published: February 2021

The adenosine A2A receptor (A2AR) represents a potential therapeutic target for neurodegenerative diseases. Aiming at the development of a positron emission tomography (PET) radiotracer to monitor changes of receptor density and/or occupancy during the A2AR-tailored therapy, we designed a library of fluorinated analogs based on a recently published lead compound (PPY). Among those, the highly affine 4-fluorobenzyl derivate (PPY1; Ki(hA2AR) = 5.3 nM) and the 2-fluorobenzyl derivate (PPY2; Ki(hA2AR) = 2.1 nM) were chosen for 18F-labeling via an alcohol-enhanced copper-mediated procedure starting from the corresponding boronic acid pinacol ester precursors. Investigations of the metabolic stability of [18F]PPY1 and [18F]PPY2 in CD-1 mice by radio-HPLC analysis revealed parent fractions of more than 76% of total activity in the brain. Specific binding of [18F]PPY2 on mice brain slices was demonstrated by in vitro autoradiography. In vivo PET/magnetic resonance imaging (MRI) studies in CD-1 mice revealed a reasonable high initial brain uptake for both radiotracers, followed by a fast clearance.
OsBRKq1, Related Grain Size Mapping, and Identification of Grain Shape Based on QTL Mapping in Rice
Authors: Park Jae-Ryoung , Resolus Dany , Kim Kyung-Min
Published: February 2021

The world population is growing rapidly, and food shortage remains a critical issue. Quantitative trait locus (QTL) mapping is a statistical analytical method that uses both phenotypic and genotypic data. The purpose of QTL mapping is to determine the exact gene location for various complex traits. Increasing grain weight is a way to increase yield in rice. Genes related to grain size were mapped using the Samgang/Nagdong double haploid (SNDH) populations. Grain sizes were diversely distributed in SNDH 113 populations, and OsBRKq1 was detected on chromosome 1 in an analysis of QTL mapping that used 1000 grain weight, grain length, and grain width. OsBRKq1 exhibited high sequence similarity with the brassinosteroid leucine-rich repeat-receptor kinases of Arabidopsis thaliana and Zea mays. It was also predicted to have a similar function because of its high homology. OsBRKq1 interacts with various grain-size control genes. Among the SNDH populations, the analysis of the relative expression level during the panicle formation stage of OsBRKq1 in panicles of SNDH117, which has the largest grain size, and SNDH6, which has the smallest grain size, the relative expression level was significantly increased in SNDH117 panicles. SNDH populations have been advancing generations for 10 years; various genetic traits have been fixed and are currently being used as bridging parents. Therefore, the stable expression level of OsBRKq1 was confirmed via QTL mapping. In the future, OsBRKq1 can be effectively used to increase the yield of rice and solve food problems by increasing the size of seeds.
P16 and HPV Genotype Significance in HPV-Associated Cervical Cancer—A Large Cohort of Two Tertiary Referral Centers
Authors: da Mata Sara , Ferreira Joana , Nicolás Inmaculada , Esteves Susana , Esteves Gonçalo , Lérias Sofia , Silva Fernanda , Saco Adela , Cochicho Daniela , Cunha Mário , del Pino Marta , Ordi Jaume , Félix Ana
Published: February 2021

The expression of p16 is a good surrogate of human papillomavirus (HPV) infection in HPV-associated cancers. The significance of p16 expression, HPV genotype and genera in the outcome of patients with HPV-associated cervical cancer (CC) is unclear. Our aim is to ascertain the prognostic significance of these factors. Data from 348 patients (median age: 47.5 years old) with CC, diagnosed in two referral centers, were retrospectively collected. Advanced disease (FIGO2018 IB2-IV) was present in 68% of patients. A single HPV genotype was identified in 82.8% of patients. The most common HPVs were HPV16 (69%) and HPV18 (14%). HPV genera reflected this distribution. HPV16 tumors presented at an earlier stage. P16 was negative in 18 cases (5.2%), 83.3% of which were squamous cell carcinomas. These cases occurred in older patients who tended to have advanced disease. In the univariate analysis, HPV16 (HR: 0.58; p = 0.0198), α-9 genera (HR: 0.37; p = 0.0106) and p16 overexpression (HR: 0.54; p = 0.032) were associated with better survival. HPV16 (HR: 0.63; p = 0.0174) and α-9 genera (HR: 0.57; p = 0.0286) were associated with less relapse. In the multivariate analysis, only the International Federation of Gynecology and Obstetrics (FIGO) stage retained an independent prognostic value. HPV16, α-9 genera and p16 overexpression were associated with better survival, although not as independent prognostic factors. Patients with p16-negative HPV-associated CC were older, presented with advanced disease and had worse prognosis.
Cardiac T-Tubule cBIN1-Microdomain, a Diagnostic Marker and Therapeutic Target of Heart Failure
Authors: Li Jing , Richmond Bradley , Hong TingTing
Published: February 2021

Since its first identification as a cardiac transverse tubule (t-tubule) protein, followed by the cloning of the cardiac isoform responsible for t-tubule membrane microdomain formation, cardiac bridging integrator 1 (cBIN1) and its organized microdomains have emerged as a key mechanism in maintaining normal beat-to-beat heart contraction and relaxation. The abnormal remodeling of cBIN1-microdomains occurs in stressed and diseased cardiomyocytes, contributing to the pathophysiology of heart failure. Due to the homeostatic turnover of t-tubule cBIN1-microdomains via microvesicle release into the peripheral circulation, plasma cBIN1 can be assayed as a liquid biopsy of cardiomyocyte health. A new blood test cBIN1 score (CS) has been developed as a dimensionless inverse index derived from plasma cBIN1 concentration with a diagnostic and prognostic power for clinical outcomes in stable ambulatory patients with heart failure with reduced or preserved ejection fraction (HFrEF or HFpEF). Recent evidence further indicates that exogenous cBIN1 introduced by adeno-associated virus 9-based gene therapy can rescue cardiac contraction and relaxation in failing hearts. The therapeutic potential of cBIN1 gene therapy is enormous given its ability to rescue cardiac inotropy and provide lusitropic protection in the meantime. These unprecedented capabilities of cBIN1 gene therapy are shifting the current paradigm of therapy development for heart failure, particularly HFpEF.
Swift and Reliable “Easy Lab” Methods for the Sensitive Molecular Detection of African Swine Fever Virus
Authors: Elnagar Ahmed , Pikalo Jutta , Beer Martin , Blome Sandra , Hoffmann Bernd
Published: February 2021

African swine fever (ASF) is a contagious viral hemorrhagic disease of domestic pigs and wild boars. The disease is notifiable to the World Organisation for Animal Health (OIE) and is responsible for high mortality and serious economic losses. PCR and real-time PCR (qPCR) are the OIE-recommended standard methods for the direct detection of African swine fever virus (ASFV) DNA. The aim of our work was the simplification and standardization of the molecular diagnostic workflow in the lab. For validation of this “easy lab” workflow, different sample materials from animal trials were collected and analyzed (EDTA blood, serum, oral swabs, chewing ropes, and tissue samples) to identify the optimal sample material for diagnostics in live animals. Based on our data, the EDTA blood samples or bloody tissue samples represent the best specimens for ASFV detection in the early and late phases of infection. The application of prefilled ready-to-use reagents for nucleic acid extraction or the use of a Tissue Lysis Reagent (TLR) delivers simple and reliable alternatives for the release of the ASFV nucleic acids. For the qPCR detection of ASFV, different published and commercial kits were compared. Here, a lyophilized commercial kit shows the best results mainly based on the increased template input. The good results of the “easy lab” strategy could be confirmed by the ASFV detection in field samples from wild boars collected from the 2020 ASFV outbreak in Germany. Appropriate internal control systems for extraction and PCR are key features of the “easy lab” concept and reduce the risk of false-negative and false-positive results. In addition, the use of easy-to-handle machines and software reduces training efforts and the misinterpretation of results. The PCR diagnostics based on the “easy lab” strategy can realize a high sensitivity and specificity comparable to the standard PCR methods and should be especially usable for labs with limited experiences and resources.
Structural and Functional Characterization of the ABA-Water Deficit Stress Domain from Wheat and Barley: An Intrinsically Disordered Domain behind the Versatile Functions of the Plant Abscissic Acid, Stress and Ripening Protein Family
Authors: Yacoubi Ines , Hamdi Karama , Fourquet Patrick , Bignon Christophe , Longhi Sonia
Published: February 2021

The ASR protein family has been discovered thirty years ago in many plant species and is involved in the tolerance of various abiotic stresses such as dehydration, salinity and heat. Despite its importance, nothing is known about the conserved ABA-Water Deficit Stress Domain (ABA-WDS) of the ASR gene family. In this study, we characterized two ABA-WDS domains, isolated from durum wheat (TtABA-WDS) and barley (HvABA-WDS). Bioinformatics analysis shows that they are both consistently predicted to be intrinsically disordered. Hydrodynamic and circular dichroism analysis indicate that both domains are largely disordered but belong to different structural classes, with HvABA-WDS and TtABA-WDS adopting a PreMolten Globule-like (PMG-like) and a Random Coil-like (RC-like) conformation, respectively. In the presence of the secondary structure stabilizer trifluoroethanol (TFE) or of increasing glycerol concentrations, which mimics dehydration, the two domains acquire an α-helical structure. Interestingly, both domains are able to prevent heat- and dehydration-induced inactivation of the enzyme lactate dehydrogenase (LDH). Furthermore, heterologous expression of TtABA-WDS and HvABA-WDS in the yeast Saccharomyces cerevisiae improves its tolerance to salt, heat and cold stresses. Taken together our results converge to show that the ABA-WDS domain is an intrinsically disordered functional domain whose conformational plasticity could be instrumental to support the versatile functions attributed to the ASR family, including its role in abiotic stress tolerance. Finally, and after validation in the plant system, this domain could be used to improve crop tolerance to abiotic stresses.