bluuebunny/crossref_metadata_2025 huggingface dataset:
LDL Cholesterol Variability Impacts the Prognosis of Patients with Chronic Ischemic Heart Disease: A Real-World Italian Experience
Authors: Faggiano Pompilio , Ruscica Massimiliano , Bettari Sara , Cherubini Antonella , Carugo Stefano , Corsini Alberto , Barbati Giulia , Di Lenarda Andrea
Published: September 2023

Epidemiologic, genetic, and clinical intervention studies have indisputably shown that low-density lipoprotein cholesterol (LDL-C) is causal in the development of atherosclerotic cardiovascular disease (ASCVD). However, LDL-C variability could be related to increased ASCVD risk in patients already treated with statins. The aim of the present retrospective real-life study was to assess the prognostic impact of LDL-C variability on all-cause mortality and cardiovascular hospitalizations in patients with stable cardiovascular artery disease. A total of 3398 patients were enrolled and followed up for a median of 56 months. Considering LDL-C < 70 mg/dL as the therapeutical target, during follow-up, the percentage of patients who achieved this goal raised from 20.7% to 31.9%. In total, 1988 events were recorded, of which 428 were all-cause deaths and 1560 were cardiovascular hospitalizations. At the last medical examination, each increase in LDL-C levels of 20 mg/dL corresponded to a 6% raise in the risk of any event (HR 1.06; 95%CI, 1.03 to 1.09). In conclusion, our real-world study supports the hypothesis that a continuous and progressive downward trend in LDL-C levels is needed to achieve and maintain a cardiovascular benefit, at least in secondary prevention.
Treatment of Vulvovaginal Laxity by Electroporation: The Jett Plasma Medical for Her II Study
Authors: Fait Tomas , Baltazár Tivadar , Bubenickova Leona , Kestranek Jan , Stepan Martin , Muller Miroslav , Turcan Pavel
Published: September 2023

Introduction: Vaginal laxity is a widespread and undertreated medical condition associated especially with vaginal parity. Aim: To evaluate the efficacy and safety of electroporation therapy treatment of vulvovaginal laxity by the Jett Plasma for Her II device. Methods: The Jett Plasma for Her II Study is a multicentric, prospective, randomized, single-blinded, and controlled study. Women presenting with vaginal laxity were randomized to receive electroporation therapy delivered to the vaginal tissue (active—82 patients) vs. therapy with zero intensity (placebo—9 patients). Results: A total of 91 subjects whose average age was 48.69 ± 10.89 were included. Due to the results of a one-way analysis of variance, it may be concluded that in the case of the vaginal laxity questionnaire (VLQ), there is a statistically significant difference between actively treated patients and the placebo group (F1,574 = 46.91; p < 0.001). In the case of the female sexual function index (FSFI), a one-way ANOVA test also showed a statistically significant difference between the actively treated patients and the placebo group (F1,278 = 7.97; p = 0.005). In the case of the incontinence impact questionnaire-7 (IIQ-7), a one-way ANOVA test showed a statistically significant difference between the actively treated patients and the placebo group (F1,384 = 15.51; p < 0.001). It confirms that improvement of vaginal laxity is conjoined with benefits in symptoms of urinary incontinence. Biopsy performed after the end of the treatment shows an increase in the vaginal mucosa thickness by an average of 100.04% in the active group. The treatment was well tolerated with no adverse events. No topical anesthetics were required. Conclusions: Treatments of vulvovaginal laxity by electroporation therapy achieved significant and sustainable 12-month effectiveness. Responses to the questionnaires also suggest subjective improvement in self-reported sexual function, incontinence, sexual satisfaction, and urogenital distress.
Busulfan/Cyclophosphamide Compared with Melphalan as a Conditioning Regimen for Autologous Transplantation of Multiple Myeloma: A Long-Term Assessment
Authors: Zhou Shiyuan , Zhai Yingying , Yan Lingzhi , Shi Xiaolan , Shang Jingjing , Wu Depei , Fu Chengcheng , Jin Song
Published: September 2023

Background: Melphalan was poorly available in mainland China. The aim of this study is to explore the dose-adjusted busulfan/cyclophosphamide (BU/CY) as an alternative regimen in auto stem cell transplantation (ASCT) for multiple myeloma (MM). Methods: A total of 105 newly diagnosed MM patients undergoing ASCT during May 2012 and August 2017 were retrospectively analyzed. The BU/CY regimen was applied to 64 patients. Busulfan (9.6 mg/kg or 8.0 mg/kg in total) and cyclophosphamide (3.6 g/m2 or 3.0 g/m2 in total) were administered according to the creatinine clearance rate (CCR). A high-dose melphalan (HDMEL) regimen (200 mg/m2) was given to the other 41 patients. Results: At a median follow-up of 65 (1~119) months, estimated overall survival (OS) and progression-free survival (PFS) at 104 months in the BU/CY and HDMEL groups were 35.6% vs. 20.5% (p = 0.263) and 20.2% vs. 2.4% (p = 0.035), respectively. The median overall survival (OS) and PFS of the HDMEL and BU/CY groups were 55 vs. 70.5 months and 26 vs. 46.5 months, respectively. In multivariate analysis, the BU/CY regimen was found to be the only protective factor for PFS. No lethal toxicity was found in the BU/CY group, and treatment-related mortality (TRM) in 100 days was similar to the HDMEL group. Conclusions: MM patients may also benefit from the dose-adjusted BU/CY regimen.
Stress and Depressive and Anxiety Symptoms in the General Population and in SARS-CoV-2-Infected Patients—Findings from a Population-Based Three-Wave Study
Authors: Wallis Hannah , Elgner Melanie , Schurr Marisa , Giel Katrin Elisabeth , Martus Peter , Paul Gregor , Jürgensen Jan Steffen , Allwang Christine , Mikolajczyk Rafael , Galante-Gottschalk Annette , Ehehalt Stefan , Junne Florian , Binneböse Marius
Published: September 2023

Objective: Understanding factors that impaired mental health during the COVID-19 pandemic is extremely relevant in order to mitigate long-term consequences of the pandemic and to promote resilience in future crises. Method: Data were collected in southern Germany in a population-based survey study (CoKoS) with three times of measurement in May 2020, November 2020 and July 2021. Predictors of depressive and anxiety symptoms were measured with a short version of the Patient Health Questionnaire (PHQ-4) in the general population (N = 758) and individuals who were infected with SARS-CoV-2 in the beginning of the pandemic (N = 412). We investigated differences between both samples and how stress components (worry, tension, demands and joy) measured with the Perceived Stress Questionnaire (PSQ) varied with depressive and anxiety symptoms over time. Three linear mixed models (GLMMs) were fitted to predict the PHQ-4 stepwise, including sociodemographic variables and stress (PSQ). Results: Depressive and anxiety symptoms increased from May 2020 to November 2020 and remained stable until July 2021. There were no differences between people with SARS-CoV-2 infection and the general population. Those with a pre-existing disease and lower education reported higher levels of depressive and anxiety symptoms. Stress explained a substantial fraction of variance in depressive and anxiety symptoms. The stress component worry emerged as the strongest predictor of depressive and anxiety symptoms, whereas joy seemed to buffer these symptoms. Conclusions: The results suggest that mitigating people’s worry and increasing joy may promote resilience in future crises. Future studies should assess mental health interventions targeted at vulnerable groups, such as those with lower socioeconomic status and poorer health.
Vogt-Koyanagi-Harada Disease and COVID
Authors: Manni Priscilla , Saturno Maria Carmela , Accorinti Massimo
Published: September 2023

Vogt–Koyanagi–Harada (VKH) is a rare multisystem inflammatory disease affecting the eyes, ears, brain, skin, and hair. The Coronavirus Disease 2019 (COVID-19) is a new contagious infection that might trigger the onset of VKH disease, as previously proposed for other viruses. Moreover, after the mass vaccination against SARS-CoV-2 worldwide, cases of VKH disease associated with COVID-19 vaccination have been reported. We present an overview of VKH and a comprehensive literature revision of all the VKH cases described after COVID-19 infection and vaccination, adding our experience. No differences have been found considering epidemiology and clinical findings of the disease compared to those reported in the no-COVID era. All of the patients promptly responded to systemic and local corticosteroid therapy with a good final visual prognosis. Different possible pathogenetic mechanisms underlying the onset of VKH after COVID-19 vaccination are discussed, while the presence of the HLA DR4 antigen as a genetic predisposition for the onset of the disease after COVID-19 infection and vaccination is proposed. VKH disease is one of the most frequently reported uveitic entities after COVID-19 vaccination, but a good response to therapy should not discourage vaccination. Nevertheless, ophthalmologists should be alerted to the possibility of VKH occurrence or relapse after COVID-19 vaccination, especially in genetically predisposed subjects.
Characteristics and Effectiveness of Mobile- and Web-Based Tele-Emergency Consultation System between Rural and Urban Hospitals in South Korea: A National-Wide Observation Study
Authors: Choi WooSung , Lim YongSu , Heo Tag , Lee SungMin , Kim Won , Kim Sang-Chul , Kim YeonWoo , Kim JaeHyuk , Kim Hyun , Kim HyungIl , Lee TaeHun , Kim Chol
Published: September 2023

(1) Background: The government of South Korea has established a nationwide web- and mobile-based emergency teleconsultation network by designating urban and rural hospitals. The purpose of this study is to analyze the characteristics and effectiveness of the tele-emergency system in South Korea. (2) Methods: Tele-emergency consultation cases from May 2015 to December 2018 were analyzed in the present study. The definition of a tele-emergency in the present study is an emergency consultation between doctors in rural and urban hospitals via a web- and mobile-based remote emergency consultation system (RECS). Consultations through an RECS are grouped into three categories: medical procedure or treatment guidance, image interpretation, and transportation requests. The present study analyzed the characteristics of the tele-emergency system and the reduction in unnecessary transportation (RUT). (3) Results: A total of 2604 cases were analyzed in the present study from 2985 tele-emergency consultation cases. A total of 381 cases were excluded for missing data. Consultations for image interpretation were the most common in trauma cases (71.3%), while transfer requests were the most common in non-trauma cases (50.3%). Trauma patients were more frequently admitted to rural hospitals or discharged and followed up with at rural hospitals (20.3% vs. 40.5%) after consultations. In terms of disease severity, non-severe cases were statistically higher in trauma cases (80.6% vs. 59.4%; p < 0.001). The RUT was statistically highly associated with trauma cases (60.8% vs. 42.8%; p < 0.001). In an analysis that categorized cases by region, a statistically higher proportion of transportation was used in island regions (69.9% vs. 49.5%; p < 0.003). More RUT was associated with non-island regions (30.1% vs. 50.5%; p = 0.001). (4) Conclusions: The tele-emergency system had a great role in reducing unnecessary patient transportation in non-severe trauma cases and non-island rural area emergency cases. Further research is needed for a cost/benefit analysis and clinical outcomes.
Immediate and Long-Term Effects of Hyperbaric Oxygenation in Patients with Long COVID-19 Syndrome Using SF-36 Survey and VAS Score: A Clinical Pilot Study
Authors: Lindenmann Joerg , Porubsky Christian , Okresa Lucija , Klemen Huberta , Mykoliuk Iurii , Roj Andrej , Koutp Amir , Kink Eveline , Iberer Florian , Kovacs Gabor , Krause Robert , Smolle Josef , Smolle-Juettner Freyja Maria
Published: September 2023

(1) Background: Long COVID syndrome (LCS) is a heterogeneous long-standing condition following COVID-19 infection. Treatment options are limited to symptomatic measures, and no specific medication has been established. Hyperbaric oxygenation (HBO) has been found to have a positive impact on the treatment of COVID-19 infection. This study evaluates both the feasibility and outcome of supportive HBO in patients with LCS. (2) Methods: Within 17 months, 70 patients with proven LCS were prospectively included. Each patient underwent a cycle of 10 subsequent HBO treatment sessions administered for 75 min at 2.2 atmospheres. Evaluation of the patients was performed before the first and after the last HBO session and 3 months afterwards. Statistical evaluation was based on an intention-to-treat analysis using Fisher’s exact test and Student’s t-test for paired samples. (3) Results: In total, 59 patients (33 females, 26 males; mean age: 43.9 years; range: 23–74 years; median: 45.0) were evaluable. After HBO, a statistically significant improvement of physical functioning (p < 0.001), physical role (p = 0.01), energy (p < 0.001), emotional well-being (p < 0.001), social functioning (p < 0.001), pain (p = 0.01) and reduced limitation of activities (p < 0.001) was confirmed. (4) Conclusions: Physical functioning and both the physical and emotional role improved significantly and sustainably, suggesting HBO as a promising supportive therapeutic tool for the treatment of LCS.
When Do Korsakoff Patients Justify Immoral Behaviors? The Influence of Premorbid Delinquency and Self-Other Perspectives in Moral Decision-Making and Moral Reasoning
Authors: Vlot Nairobi , Postma Albert , Oudman Erik
Published: September 2023

Korsakoff’s syndrome (KS) is a chronic neuropsychiatric disorder caused by a vitamin B1 deficiency. KS is characterized by profound amnesia and often accompanied by poor executive functioning, decreased social-cognitive abilities, and difficulties in behavioral regulation. As moral behaviors and attitudes may provide insight in socio-behavioral interactions, the current study aimed to evaluate everyday moral maturity by administering self- versus other-oriented moral dilemmas in a group of KS patients (n = 20) and healthy controls (n = 20). Responses were scored according to the Kohlberg stages of moral reasoning. Furthermore, we assessed premorbid delinquency and current neurocognitive functioning as possible relevant factors. Our results show that KS patients were prone to lower levels of moral maturity when confronted with moral dilemmas relating to themselves, compared to dilemmas relating to (un)personal others in KS patients, while healthy subjects showed an opposite pattern. Moral immaturity could find its origin already before the onset of the KS diagnosis, as suggested by the elevated premorbid levels of delinquent behavior and correlation between premorbid delinquency and moral maturity in KS. Lower moral maturity could therefore be a possible predisposing factor to both delinquency and later development of Korsakoff’s syndrome.
Usefulness of the Veterans Health Administration COVID-19 (VACO) Index for Predicting Short-Term Mortality among Patients of the COLOS Study
Authors: Matera-Witkiewicz Agnieszka , Krupińska Magdalena , Doroszko Adrian , Trocha Małgorzata , Giniewicz Katarzyna , Kujawa Krzysztof , Rabczyński Maciej , Obremska Marta , Kuznik Edwin , Lubieniecki Pawel , Adamik Barbara , Kaliszewski Krzysztof , Kiliś-Pstrusińska Katarzyna , Pomorski Michał , Protasiewicz Marcin , Madziarski Marcin , Sokołowski Janusz , Jankowska Ewa A. , Madziarska Katarzyna
Published: September 2023

Advanced age is known to be a predictor with COVID-19 severity. Understanding of other disease progression factors may shorten the time from patient admission to applied treatment. The Veterans Health Administration COVID-19 (VACO index) was assumed to additionally anticipate clinical results of patients hospitalized with a proven infection caused by the SARS-CoV-2 virus. Methods: The medical records of 2183 hospitalized patients were retrospectively analyzed. Patients were divided into four risk-of-death categories: low risk, medium risk, high-risk, and extreme risk depending on their VACO index calculation. Results: Significant differences in the mortality at the hospital after three months of discharge and six months after discharge were noticed. For the patients in the extreme-risk group, mortality reached 37.42%, 62.81%, and 78.44% for in-hospital, three months of discharge, and six months of discharge, respectively. The mortality marked as high risk reached 20.38%, 37.19%, and 58.77%. Moreover, the secondary outcomes analysis acknowledged that patients classified as extreme risk were more likely to suffer from cardiogenic shock, myocardial infarction, myocardial injury, stroke, pneumonia, acute kidney injury, and acute liver dysfunction. Patients at moderate risk were more often admitted to ICU when compared to other patients. Conclusions: The usage of the VACO index, combined with an appropriate well-defined medical interview and past medical history, tends to be a helpful instrument in order to predict short-term mortality and disease progression based on previous medical records.
Transcatheter Aortic Valve Replacement in Degenerated Perceval Bioprosthesis: Clinical and Technical Aspects in 32 Cases
Authors: Concistrè Giovanni , Gasbarri Tommaso , Ravani Marcello , Al Jabri Anees , Trianni Giuseppe , Bianchi Giacomo , Margaryan Rafik , Chiaramonti Francesca , Murzi Michele , Kallushi Enkel , Varone Egidio , Simeoni Simone , Leone Alessandro , Farneti Andrea , Berti Sergio , Solinas Marco
Published: September 2023

Background: Sutureless aortic bioprostheses are increasingly being used to provide shorter cross-clamp time and facilitate minimally invasive aortic valve replacement. As the use of sutureless valves has increased over the past decade, we begin to encounter their degeneration. We describe clinical outcomes and technical aspects in patients with degenerated sutureless Perceval (CorCym, Italy) aortic bioprosthesis treated with valve-in-valve transcatheter aortic valve replacement (VIV-TAVR). Methods: Between March 2011 and March 2023, 1310 patients underwent aortic valve replacement (AVR) with Perceval bioprosthesis implantation. Severe bioprosthesis degeneration treated with VIV-TAVR occurred in 32 patients with a mean of 6.4 ± 1.9 years (range: 2–10 years) after first implantation. Mean EuroSCORE II was 9.5 ± 6.4% (range: 1.9–35.1%). Results: Thirty of thirty-two (94%) VIV-TAVR were performed via transfemoral and two (6%) via transapical approach. Vascular complications occurred in two patients (6%), and mean hospital stay was 4.6 ± 2.4 days. At mean follow-up of 16.7 ± 15.2 months (range: 1–50 months), survival was 100%, and mean transvalvular pressure gradient was 18.7 ± 5.3 mmHg. Conclusion: VIV-TAVR is a useful option for degenerated Perceval and appears safe and effective. This procedure is associated with good clinical results and excellent hemodynamic performance in our largest single-center experience.
B Cell Tolerance and Targeted Therapies in SLE
Authors: Parodis Ioannis , Long Xuan , Karlsson Mikael C. I. , Huang Xin
Published: September 2023

Systemic Lupus Erythematosus (SLE) is a chronic systemic autoimmune disease of high clinical and molecular heterogeneity, and a relapsing-remitting pattern. The disease is currently without cure and more prevalent in women. B cell tolerance and production of autoantibodies are critical mechanisms that drive SLE pathophysiology. However, how the balance of the immune system is broken and how the innate and adaptive immune systems are interacting during lupus-specific autoimmune responses are still largely unknown. Here, we review the latest knowledge on B cell development, maturation, and central versus peripheral tolerance in connection to SLE and treatment options. We also discuss the regulation of B cells by conventional T cells, granulocytes, and unconventional T cells, and how effector B cells exert their functions in SLE. We also discuss mechanisms of action of B cell-targeted therapies, as well as possible future directions based on current knowledge of B cell biology.
Association of Blood Leukocytes and Hemoglobin with Hospital Mortality in Acute Pulmonary Embolism
Authors: Obradovic Slobodan , Dzudovic Boris , Subotic Bojana , Salinger Sonja , Matijasevic Jovan , Benic Marija , Kovacevic Tamara , Kovacevic-Kuzmanovic Ana , Mitevska Irena , Miloradovic Vladimir , Jevtic Ema , Neskovic Aleksandar
Published: September 2023

This study aimed to assess the prognostic significance of total leukocyte count (TLC) and hemoglobin (Hb) levels upon admission for patients with acute pulmonary embolism (PE), considering the European Society of Cardiology (ESC) model for mortality risk. 1622 patients from a regional PE registry were included. Decision tree statistics were employed to evaluate the prognostic value of TLC and Hb, both independently and in conjunction with the ESC model. The results indicated all-cause and PE-related in-hospital mortality rates of 10.7% and 6.5%, respectively. Subgrouping patients based on TLC cut-off values (≤11.2, 11.2–16.84, >16.84 × 109/L) revealed increasing all-cause mortality risks (7.0%, 11.8%, 30.2%). Incorporating Hb levels (≤126 g/L or above) further stratified the lowest risk group into two strata with all-cause mortality rates of 10.1% and 4.7%. Similar trends were observed for PE-related mortality. Notably, TLC improved risk assessment for intermediate–high-risk patients within the ESC model, while Hb levels enhanced mortality risk stratification for lower-risk PE patients in the ESC model for all-cause mortality. In conclusion, TLC and Hb levels upon admission can refine the ESC model’s mortality risk classification for patients with acute PE, providing valuable insights for improved patient management.
Cyberattacks in Smart Grids: Challenges and Solving the Multi-Criteria Decision-Making for Cybersecurity Options, Including Ones That Incorporate Artificial Intelligence, Using an Analytical Hierarchy Process
Authors: Bouramdane Ayat-Allah
Published: September 2023

Smart grids have emerged as a transformative technology in the power sector, enabling efficient energy management. However, the increased reliance on digital technologies also exposes smart grids to various cybersecurity threats and attacks. This article provides a comprehensive exploration of cyberattacks and cybersecurity in smart grids, focusing on critical components and applications. It examines various cyberattack types and their implications on smart grids, backed by real-world case studies and quantitative models. To select optimal cybersecurity options, the study proposes a multi-criteria decision-making (MCDM) approach using the analytical hierarchy process (AHP). Additionally, the integration of artificial intelligence (AI) techniques in smart-grid security is examined, highlighting the potential benefits and challenges. Overall, the findings suggest that “security effectiveness” holds the highest importance, followed by “cost-effectiveness”, “scalability”, and “Integration and compatibility”, while other criteria (i.e., “performance impact”, “manageability and usability”, “compliance and regulatory requirements”, “resilience and redundancy”, “vendor support and collaboration”, and “future readiness”) contribute to the evaluation but have relatively lower weights. Alternatives such as “access control and authentication” and “security information and event management” with high weighted sums are crucial for enhancing cybersecurity in smart grids, while alternatives such as “compliance and regulatory requirements” and “encryption” have lower weighted sums but still provide value in their respective criteria. We also find that “deep learning” emerges as the most effective AI technique for enhancing cybersecurity in smart grids, followed by “hybrid approaches”, “Bayesian networks”, “swarm intelligence”, and “machine learning”, while “fuzzy logic”, “natural language processing”, “expert systems”, and “genetic algorithms” exhibit lower effectiveness in addressing smart-grid cybersecurity. The article discusses the benefits and drawbacks of MCDM-AHP, proposes enhancements for its use in smart-grid cybersecurity, and suggests exploring alternative MCDM techniques for evaluating security options in smart grids. The approach aids decision-makers in the smart-grid field to make informed cybersecurity choices and optimize resource allocation.
Automatic Jordanian License Plate Detection and Recognition System Using Deep Learning Techniques
Authors: Aqaileh Tharaa , Alkhateeb Faisal
Published: September 2023

Recently, the number of vehicles on the road, especially in urban centres, has increased dramatically due to the increasing trend of individuals towards urbanisation. As a result, manual detection and recognition of vehicles (i.e., license plates and vehicle manufacturers) become an arduous task and beyond human capabilities. In this paper, we have developed a system using transfer learning-based deep learning (DL) techniques to identify Jordanian vehicles automatically. The YOLOv3 (You Only Look Once) model was re-trained using transfer learning to accomplish license plate detection, character recognition, and vehicle logo detection. In contrast, the VGG16 (Visual Geometry Group) model was re-trained to accomplish the vehicle logo recognition. To train and test these models, four datasets have been collected. The first dataset consists of 7035 Jordanian vehicle images, the second dataset consists of 7176 Jordanian license plates, and the third dataset consists of 8271 Jordanian vehicle images. These datasets have been used to train and test the YOLOv3 model for Jordanian license plate detection, character recognition, and vehicle logo detection. In comparison, the fourth dataset consists of 158,230 vehicle logo images used to train and test the VGG16 model for vehicle logo recognition. Text measures were used to evaluate the performance of our developed system. Moreover, the mean average precision (mAP) measure was used to assess the YOLOv3 model of the detection tasks (i.e., license plate detection and vehicle logo detection). For license plate detection, the precision, recall, F-measure, and mAP were 99.6%, 100%, 99.8%, and 99.9%, respectively. While for character recognition, the precision, recall, and F-measure were 100%, 99.9%, and 99.95%, respectively. The performance of the license plate recognition stage was evaluated by evaluating these two sub-stages as a sequence, where the precision, recall, and F-measure were 99.8%, 99.8%, and 99.8%, respectively. Furthermore, for vehicle logo detection, the precision, recall, F-measure, and mAP were 99%, 99.6%, 99.3%, and 99.1%, respectively, while for vehicle logo recognition, the precision, recall, and F-measure were 98%, 98%, and 98%, respectively. The performance of the vehicle logo recognition stage was evaluated by evaluating these two sub-stages as a sequence, where the precision, recall, and F-measure were 95.3%, 99.5%, and 97.4%, respectively.
Analysis of the Interannual Variability of Pacific Swell Pools
Authors: Zhang Xin , Wu Kejian , Li Rui , Li Dongze , Zhang Shuai , Zhang Ruyan , Li Shuo , Dong Xianghui
Published: September 2023

The investigation of the propagation of swells throughout the ocean has long been a subject of significant interest in physical oceanography. This paper investigates the interannual variability of the Pacific swell pools and examines the factors contributing to their formation using the European Centre for Medium-Range Weather Forecasts (ECMWF) ERA5 re-analysis dataset. Our results show that the interaction between swell propagation and wind fields influences the formation and development of Pacific swell pools. The eastern and southern Pacific swell pools are mainly caused by the northward propagation of swells from the South Pacific; the western and northern swell pools are primarily influenced by the southward propagation of swells from the North Pacific; and the central part of the swell pools is the result of the combined impact of both swell propagation from the north and south Pacific. The size of the swell pools in the Pacific Ocean is at its maximum in the northern hemisphere during the winter (December, January, and February) and at its minimum during the summer (June, July, and August). Due to the impact of the low-pressure systems, the swell pools in the winter hemisphere are relatively small, while the swell pools in the summer hemisphere are significantly larger. There is a relationship between the swell pools and ENSO events. When an El Niño event (La Niña event) occurs, the swells propagating to the low latitudes of the Pacific Ocean from high latitudes will strengthen (weaken), resulting in an increase (decrease) in the size of the swell pools. Analyzing the spatial and temporal distribution of the swell pools is important for understanding the large-scale effect of waves.
Unusual Differences in the Pulmonary Histopathology of Mice after Intranasal Infection with Mycelial Propagules of Histoplasma capsulatum Strains Classified as LAm A2 and NAm 2 Phylogenetic Species
Authors: Pulido-Camarillo Evelyn , Sahaza Jorge H. , de Souza Pitangui Nayla , Mendes-Giannini Maria José S. , Fusco-Almeida Ana M. , Pérez-Torres Armando , Taylor Maria Lucia
Published: September 2023

The ascomycete Histoplasma capsulatum is the causative agent of systemic respiratory mycosis histoplasmosis, which sometimes develops acute disseminated or chronic clinical forms, with the latter usually associated with granuloma formation. The present report shows differential histopathological changes in the pulmonary inflammatory response of mice infected intranasally with the mycelial morphotype of H. capsulatum strains with distinct genotypes, EH-46 and G-217B, classified as LAm A2 and NAm 2 phylogenetic species, respectively. Infected male BALB/c mice were sacrificed at different postinfection times, and their serial lung sections were stained with periodic acid–Schiff and analyzed via microscopy. In mice infected with the LAm A2 strain, the results showed progressive changes in the inflammatory infiltrate of the lung parenchyma during the first hours and days postinfection as well as granulomas with macrophages containing intracellular yeast cells, which prevailed at 14 and 21 days postinfection. Bronchiolar-associated lymphoid tissue was induced in mice infected with both strains, primarily in mice infected with the NAm 2 strain. Several lung sections from mice infected with the LAm A2 strain showed PAS-positive yeast cells aggregated in a perinuclear crown-like arrangement in macrophages from 3 h to 21 days postinfection. These findings highlight differences in the host pulmonary inflammatory response associated with distinct H. capsulatum species.
Interaction between a Martian Regolith Simulant and Fungal Organic Acids in the Biomining Perspective
Authors: Vezzola Michele , Tosi Solveig , Doria Enrico , Bonazzi Mattia , Alvaro Matteo , Sanfilippo Alessio
Published: September 2023

The aim of this study was to evaluate the potential of Aspergillus tubingensis in extracting metals from rocks simulating Martian regolith through biomining. The results indicated that the fungal strain produced organic acids, particularly oxalic acid, in the first five days, leading to a rapid reduction in the pH of the culture medium. This acidic medium is ideal for bioleaching, a process that employs acidolysis and complexolysis to extract metals from rocks. Additionally, the strain synthesized siderophores, molecules capable of mobilizing metals from solid matrices, as verified by the blue CAS colorimetric test. The secretion of siderophores in the culture medium proved advantageous for biomining. The siderophores facilitated the leaching of metal ions, such as manganese, from the rock matrix into the acidified water solution. In addition, the susceptibility of the Martian regolith simulant to the biomining process was assessed by determining the particle size distribution, acid composition after treatment, and geochemical composition of the rock. Although the preliminary results demonstrate successful manganese extraction, further research is required to optimize the extraction technique. To conclude, the A. tubingensis strain exhibits promising abilities in extracting metals from rocks through biomining. Its use could prove useful in future in situ mining operations and environmental remediation efforts. Further research is required to optimize the process and evaluate its feasibility on a larger scale.
Impervious Land Expansion as a Control Parameter for Climate-Resilient Planning on the Mediterranean Coast: Evidence from Greece
Authors: Lagarias Apostolos
Published: September 2023

Impervious land expansion is linked to ecosystem fragmentation and degradation, posing threats to nature conservation and multiplying climate change impacts. This is particularly true on the Mediterranean coast, where persistent urbanization is coupled with tourism development, further intensifying coastal erosion, flood risk, heat stress and biodiversity loss, while decreasing carbon sequestration. In this research, high-resolution imperviousness data were analyzed through a geospatial methodology to detect patterns and processes in a heavily burdened Mediterranean coastal area, namely Greece. The methodology was structured on a set of GIS tools, analyzing the distribution of new impervious cover between 2006 and 2018, to evaluate pressures exerted on coastal territories and on the environmental protection network. The results revealed relatively slow rates of impervious land expansion at a nationwide scale, mostly attributed to the economic recession period in Greece. However, certain locations exhibited continuing artificialization of land even within Natura 2000 areas, while future pressures on coastal territories are expected to increase due to the restarting of construction activity and the continuing dynamic of the mass tourism sector. The conclusions imply that controlling for imperviousness is important in order to develop spatial planning policies for climate resilience, which should be decisively enforced in the Mediterranean to prevent a business-as-usual scenario.
Liver Transplantation in People Living with HIV: Still an Experimental Procedure or Standard of Care?
Authors: Lynch Erica Nicola , Russo Francesco Paolo
Published: September 2023

Liver transplantation (LT) is the only curative treatment for various liver diseases, including acute liver failure, end-stage liver disease, and selected unresectable liver malignancies. Combination antiretroviral therapy has improved outcomes for people living with HIV (PLWH), transforming the status of acquired immune deficiency syndrome from a fatal disease to a chronic and manageable condition. These powerful antiviral therapies have not only increased the number of HIV+ enlisted patients by improving their survival but also made the use of HIV+ organs a viable option. In this review, we summarise current knowledge on the peculiarities of liver transplantation in PLWH. In particular, we focus on the indications, contraindications, specific considerations for treatment, and outcomes of LT in PLWH. Finally, we present available preliminary data on the use of HIV+ liver allografts.
Black Soldier Fly (Hermetia illucens) Meal as Direct Replacement of Complex Fish Feed for Rainbow Trout (Oncorhynchus mykiss) and African Catfish (Clarias gariepinus)
Authors: Bartucz Tamás , Csókás Endre , Nagy Borbála , Gyurcsák Márk Péter , Bokor Zoltán , Bernáth Gergely , Molnár József , Urbányi Béla , Csorbai Balázs
Published: September 2023

In the experiments, defatted black soldier fly meal reared on vegetable byproducts was used in the fry rearing of two economically important fish species, African catfish and rainbow trout. Both fish species were reared in a recirculation system and 0–33–66–100% of the complex fry feed was replaced by a defatted prepupae meal of black soldier flies during a 28-day feeding experiment. African catfish was reared at 25 ± 1 °C while rainbow trout was reared at 12 ± 1 °C. The results showed that the growth of African catfish was not significantly reduced when 66% of the feed was replaced by soldier fly meal (mean weight in the control fish group at the end of the experiment was 0.4632 ± 0.2469 g, while the 66% group resulted mean weights of 0.4150 ± 0.1886 g) and the survival did not show any statistically different results (mean survival in control group was 57.48 ± 13.76% while it was 56.6 ± 7.763% in the 66% group). In the case of rainbow trout, replacing the feed entirely with insect meal did not cause a decrease in weight gain (final mean weight in the control group was measured at 1.9640 ± 0.4154 g, while in the group consuming only insect meal, it was 1.9410 ± 0.4248 g) or in survival (in the control group 98.5%, while in the group consuming only insect meal 99.5%). All these preliminary results indicate that black soldier fly meal can be used directly as a nursery feed in fish farming as a partial or total replacement of complete feeds. The results showed that black soldier fly meal could replace 66% of the complex brood feed of African catfish and up to 100% of rainbow trout feed without deterioration of production results. Our experiments have therefore opened the way for further experiments on insect meal in larval rearing.
Optic Nerve Sheath Diameter: A Cross-Sectional Study of Ultrasonographic Measurement in Healthy Black South African Adults
Authors: Trollip Lindy , Alberto Kerry , Makgotloe Aubrey
Published: September 2023

Ultrasonographic optic nerve sheath diameter (ONSD) measurement is an accurate, portable, and non-invasive method of detecting raised intracranial pressure that can also reflect dynamic, real-time changes in intracranial pressure fluctuations. Various studies have shown the mean range of ONSD to vary greatly across different population groups. This study aimed to determine the mean ONSD in healthy Black South African adults. In this cross-sectional study, healthy black South African adult participants underwent optic nerve sheath ultrasound of the right eye, with the diameter being measured at 3 mm behind the retina in two different planes. The average of the two measurements was used to find the mean optic nerve sheath diameter. This measurement was compared to that found in a Canadian adult population, and the effect of age, gender, and co-morbidities on ONSD was assessed. A total of 99 participants were included in this study, of which 39 were male and 60 were female. The mean ONSD was found to be 5.1 mm (SD ± 0.33). This value was significantly higher than the mean ONSD observed in the Canadian population (p < 0.001). There was no significant difference found between the mean ONSD in males and females (p = 0.652), and both age and presence of co-morbidities were not found to significantly correlate with ONSD. (p = 0.693 and p = 0.974, respectively).
A Literature Review on the Use of Aortic Allografts in Modern Cardiac Surgery for the Treatment of Infective Endocarditis: Is There Clear Evidence or Is It Merely a Perception?
Authors: Nappi Francesco , Schoell Thibaut , Spadaccio Cristiano , Acar Christophe , da Costa Francisco Diniz Affonso
Published: September 2023

Infective valve endocarditis is caused by different pathogens and 60% of those involve the aortic valve with valve failure. Although S. aureus is recognized as the most frequently isolated causative bacterium associated with IE in high-income countries, Gram-positive cocci nevertheless play a crucial role in promoting infection in relation to their adhesive matrix molecules. The presence of pili on the surface of Gram-positive bacteria such as in different strains of Enterococcus faecalis and Streptococcus spp., grants these causative pathogens a great offensive capacity due to the formation of biofilms and resistance to antibiotics. The indications and timing of surgery in endocarditis are debated as well as the choice of the ideal valve substitute to replace the diseased valve(s) when repair is not possible. We reviewed the literature and elaborated a systematic approach to endocarditis management based on clinical, microbiological, and anatomopathological variables known to affect postoperative outcomes with the aim to stratify the patients and orient decision making. From this review emerges significant findings on the risk of infection in the allograft used in patients with endocarditis and no endocarditis etiology suggesting that the use of allografts has proved safety and effectiveness in patients with both pathologies.
Cellular and Molecular Triggers of Retinal Regeneration in Amphibians
Authors: Markitantova Yuliya V. , Grigoryan Eleonora N.
Published: September 2023

Understanding the mechanisms triggering the initiation of retinal regeneration in amphibians may advance the quest for prevention and treatment options for degenerating human retina diseases. Natural retinal regeneration in amphibians requires two cell sources, namely retinal pigment epithelium (RPE) and ciliary marginal zone. The disruption of RPE interaction with photoreceptors through surgery or injury triggers local and systemic responses for retinal protection. In mammals, disease-induced damage to the retina results in the shutdown of the function, cellular or oxidative stress, pronounced immune response, cell death and retinal degeneration. In contrast to retinal pathology in mammals, regenerative responses in amphibians have taxon-specific features ensuring efficient regeneration. These include rapid hemostasis, the recruitment of cells and factors of endogenous defense systems, activities of the immature immune system, high cell viability, and the efficiency of the extracellular matrix, cytoskeleton, and cell surface remodeling. These reactions are controlled by specific signaling pathways, transcription factors, and the epigenome, which are insufficiently studied. This review provides a summary of the mechanisms initiating retinal regeneration in amphibians and reveals its features collectively directed at recruiting universal responses to trauma to activate the cell sources of retinal regeneration. This study of the integrated molecular network of these processes is a prospect for future research in demand biomedicine.
Fabrication and Properties of a Biodegradable Zn-Ca Composite
Authors: Zhang Mengsi , Wang Xinyuan , Zhang Shuo , Wang Tiebao , Wang Xin , Liu Shuiqing , Zhao Lichen , Cui Chunxiang
Published: September 2023

In recent years, Zn and its alloys have become some of the most promising degradable metals as in vivo implants due to their acceptable biocompatibility and more suitable degradation rate compared with Mg-based and Fe-based alloys. However, the degradation rate of Zn-based materials after implantation in the body for orthopedic applications is relatively slow, leading to long-term retention of the implants after fulfilling their missions. Moreover, the excessive release of Zn2+ during the degradation process of Zn-based implants usually leads to high cytotoxicity and delayed osseointegration. To provide a feasible solution to the problem faced by Zn-based implants, a Zn-Ca composite was fabricated by an air pressure infiltration method in this work. The XRD pattern of the composite suggests that the composite is fully composed of Zn-Ca intermetallic compounds. The degradation tests in vitro show that the composite has a much higher degradation rate than pure Zn, and the high Ca content regions in the composite can preferentially degrade as sacrificial anodes. In addition, the composite can efficiently induce Ca-P deposition during immersion tests in Hank’s solution. Cytotoxicity tests indicate that L-929 cells exhibit around 82% cell viability (Grade 1) even after being cultured in the 100% extract prepared from the Zn-Ca composite for 1 day and show excellent cell viability.
The Effect of the Surface Topography of High-Speed Steel Cutting Blades Made Using Various Methods on Their Technological and Functional Properties
Authors: Kupczyk Maciej Jan , Komolka Jędrzej , Józwik Jerzy
Published: September 2023

Indication of the proper scope of application of high-speed steels (HSS) produced by conventional methods and powder metallurgy for cutting tool blades requires a thorough assessment of their properties in various cutting conditions. According to common knowledge, the properties of tools made of both types of steel are largely determined by the arrangement of carbide phases. It turns out, however, that the surface morphology of the cutting blades is at least as important for their operational properties. This was already indicated by the preliminary research of the authors of the article, in which it was found that the surface topography significantly affects their durability depending on the applied value of the cutting speed in dry machining conditions and in the presence of a cooling lubricant. As it turned out, contrary to the literature data, blades made of sintered HSS do not have better service properties in all cutting conditions than blades made by conventional methods (e.g., in forging and hot rolling processes). Hence, it was necessary to determine the justified range of use of both types of steel for cutting blades. Cutting blades with a similar chemical composition from conventional high-speed steel and those produced in the powder metallurgy process were tested. Wear curves were determined on the basis of the tests. On this basis, for the assumed value of the blunting index, the tool life of the cutting edges was determined during the cutting of selected structural steel, commonly used for high-strength machine parts. Tests of the cutting ability of the blades were carried out without and with the use of a cooling--lubricating liquid. In order to correctly interpret the obtained results of the wear and durability tests of the cutting edges, verification tests were carried out. A detailed analysis of the research material made it possible to determine the recommended range of applicability of conventional and sintered HSS for the tested case.
Effect of TiC Content on Microstructure and Wear Performance of 17-4PH Stainless Steel Composites Manufactured by Indirect Metal 3D Printing
Authors: Huang Xiao , Mei Shuo , Li Yazhi , Li Mingyang , Zhou Shujun , Shang Hongfei
Published: September 2023

In order to improve the performance of 17-4PH under wear conditions (e.g., gears, etc.) and reduce the cost of metal additive manufacturing, TiC needs to be added to 17-4PH to improve its wear resistance. Micron-sized TiC-reinforced 17-4PH stainless steel composites with different contents (0–15 wt%) have been prepared by fused filament fabrication 3D printing for the first time. The effects of TiC content on the structure and properties of composites were studied by XRD, SEM, and sliding wear. The obtained results show that the microstructure of TiC-reinforced 17-4PH stainless steel composites mainly consists of austenite. With the increase in TiC content, the grain size is obviously refined, and the average grain size decreases from 65.58 μm to 19.41 μm. The relative densities of the composites are maintained above 95% with the addition of TiC. The interfaces between TiC particles and the 17-4PH matrix are metallurgical bonds. The hardness of the composites increases and then decreases with increasing TiC content, and the maximum hardness (434 HV) is obtained after adding 10 wt.% of TiC content. The wear rate of the composites was reduced from 2.191 × 10−5 mm3 /(N‧m) to 0.509 × 10−5 mm3 /(N‧m), which is a 3.3-fold increase in wear resistance. The COF value declines with the addition of TiC. The reasons for the significant improvement in the composites’ performance are fine grain strengthening, solid solution strengthening, and second phase strengthening. The wear mechanisms are mainly abrasive and adhesive wear. Compared to the 10 wt% TiC composites, the 15 wt% TiC composites show limited improvement in wear resistance due to more microcracks and TiC agglomeration.