bluuebunny/crossref_metadata_2025 huggingface dataset:
Laboratory diagnostics of Acanthamoeba spp. infections
Authors: Bojanowska Aleksandra , Bożym Anna , Koper-Lenkiewicz Olga M. , Kamińska Joanna , Kemona Halina , Matowicka-Karna Joanna
Published: February 2021

Protozoa Acanthamoeba castellanii are single-celled, free-living, cosmopolitan amoebae that enter the body mainly through the mucous
membrane of the mouth, nasal cavity, cornea, respiratory system, damaged skin and intestinal mucosa. In addition, they can be vectors
for microorganisms such as bacteria, viruses, fungi and protozoa. In the life cycle A. castellanii may take the form of trophozoites and
cyst tenacity to environmental conditions. This amoeba is an etiologic agent of dangerous human diseases: Acanthamoeba keratitis
(AK), granulomatous amoebic encephalitis (GAE), pneumonia or changes in other organs, such as the liver, kidneys and skin. The aim of
the article is to present current knowledge about laboratory diagnosis of Acanthamoeba spp. infection, which is based on the search for
trophozoites and cysts in the material collected from the patient. In the case of suspected GAE, it is also advisable to study cerebrospinal
fluid sediment and perform imaging tests such as computed tomography or magnetic resonance imaging. In the case of Acanthamoeba
keratitis, an amoeba culture (obtained from a biopsy or corneal scrapings) is established in vitro, and then identified by light microscopy.
The methods of molecular biology are also useful in detecting, identifying, and determining the potential pathogenic abilities
of the amoebas. After the detection of Acanthamoeba spp., it is important to differentiate pathogenic isolates from non-pathogenic
ones. From a clinical point of view, to diagnose Acanthamoeba spp. infection it is necessary only to identify the type of organism and
to determine the pathogenicity of the isolate.
Procalcitonin as a diagnostic marker of infections and inflammation
Authors: Kolasińska Weronika , Jankowska-Kulawy Agnieszka
Published: February 2021

Infections are quite common, especially in long-term hospitalized patients. Eearly differential diagnosis of severe bacterial and viral
infections in patients in severe or critical condition is particularly important. Procalcitonin is a good and, above all, early marker of sepsis
and generalized inflammatory states. 85% sensitivity and 91% specificity of this study were shown in the differentiation patients with
systemic inflammatory response syndrome noninfectious and sepsis defined as a systemic inflammatory response syndrome induced
by infection. The usage of procalcitonin assays in clinical algorithms may accelerate the diagnosis of infectious conditions, reduce the
abuse of antibiotics and optimize therapy with these drugs. Therefore, the determination of procalcitonin concentration is increasingly
used in clinical practice.
The importance of cooperation between cytogenetic and molecular laboratory
in the genetic testing of hemato-oncological diseases on the example
of a pediatric patient with acute myeloid leukemia (AML)
Authors: Książek Teofila , Szewczyk Katarzyna , Grabowska Agnieszka , Grabowska Agnieszka , Bik-Multanowski Mirosław , Sadowska Beata , Kaczówka Przemysław
Published: February 2021

The genetic profiling of leukemic cells is currently the standard in routine diagnosis of haematopoietic malignancies. Detection of
characteristic genetic abnormalities is extremely important in establishing the correct diagnosis and selecting the optimal treatment
for the patient. In this paper we outline the problems of cytogenetic and molecular methods applied in the oncogenetic diagnostics.
Moreover, we emphasize advantages and indicate limitations of these methods. Additionally, we present their practical use on the example
of a comprehensive analysis of genetic changes in leukemic cells in a pediatric patient diagnosed with acute myeloid leukemia
(AML). Simultaneous, proper interpretation of the results of all performed analysis – classical karyotype, fluorescence in situ hybridization
(FISH), analysis of the expression of fusion genes and molecular karyotyping by microarrays, eventually led to the selection of optimal
treatment which take into account the identified adverse genetic changes of the described patient.
Assessment of HOMA1-IR, Matsuda and ISSI-2 indices in relation to the metabolic syndrome features and oral glucose tolerance test in young people
Authors: Płaczkowska Sylwia , Kokot Izabela , Pawlik-Sobecka Lilla , Piwowar Agnieszka
Published: February 2021

Background: Insulin resistance and reduced ability of pancreatic beta cells to secrete insulin preside carbohydrate disorders development and this condition is one of the stages in the type 2 diabetes development. Indirect indices of insulin resistance, sensitivity and pancreatic beta cells function, are used in clinical practice. They are calculated based on glucose and insulin concentration under fasting and postprandial condition.

Aim: The aim of this study was to examine relationship between HOMA1-IR, Matsuda Index, and ISSI-2 with metabolic syndrome (MS) features and shape of glycemic curve in young, potentially healthy people.

Material and method: The study group consisted of 152 volunteers (108 women, 44 men) aged 19-28. Participants underwent the questionnaire, anthropometric and arterial blood pressure examination. In blood samples under fasting condition lipid profiles, glucose, and insulin were measured. Glucose and insulin were measured also in 60 and 120 minutes of Oral Glucose Tolerance Test (OGTT). Based on the results, MS features were identified and HOMA1-IR, Matsuda Index and ISSI-2 values were calculated.

Results: The value of HOMA1-IR was significant higher in patients with metabolic syndrome while lower values of Matsuda and ISSI-2 were observed in participants with the MS as well as with glucose concentration in 120-minute of OGTT higher than under fasting condition.

 Conclusions: MS is associated with an increase in hepatic insulin resistance. Both MS and retardation of glucose returning to fasting values during OGTT are related to peripheral insulin resistance and reduction of pancreatic beta cell ability to insulin secretion.
Cardiac AL Amyloidosis – the role of serum Immunoglobulin Free Llight Chain assay (sFLC) in diagnosis and prognostic classification
Authors: Czyżewska Emilia , Rodziewicz-Lurzyńska Anna , Bobilewicz Dagna
Published: February 2021

Systemic immunoglobulin light chain amyloidosis (AL amyloidosis) is a cancer in which the amyloid fibrils are formed from amyloid immunoglobulin free light chain (FLC) κ or λ, produced by a plasma cell clone. The created protein adopts the structure of β-sheets, and depositing in tissues and organs leads to their function impairment. Cardiomyopathies are the main cause of death in patients with amyloidosis. Diagnosis of cardiac involvement is too late owing to nonspecific symptoms and no early characteristic changes observed in imaging studies. The currently valid prognostic classification of AL amyloidosis created by Kumar et al. is based on biochemical parameters evaluating function and the degree of myocardial damage: concentration of troponin (Tn) and N-terminal fragment of B-type natriuretic peptide (NT-proBNP) and the value of dFLC, representing the difference between the concentration of serum FLC (sFLC) involved and not involved in the neoplastic process. There are reports indicating that myocardial dysfunction in AL amyloidosis stems not only from the amyloid deposit in the organ, but it is also the result of the cardiotoxicity amyloid precursor – FLC, circulating in the blood.
The use of blood components in the University Clinic in Gdansk in 2006 – 2010
Authors: Skibowska-Bielińska Anna , Robakowska Marlena , Jakubowski Zenon , Skoniecka Izabela , Tyrańska-Fobke Anna , Ślęzak Daniel
Published: February 2021

Introduction: The aim of this paper was to present the blood components management at the University Clinical Center in Gdansk in 2006-2010. The blood and its components in the University Clinical Center (UCK) are administered in the Transfusion Clinical Laboratory of the Central Clinical Laboratory.
Materials and Methods: Material was the data obtained from the LSI-Bank Blood Bank computer system. The database contained results of pre-transfusion studies of blood recipients and donations, and determined the number of issues of blood components (red blood cells, platelets and plasma) from the NHC in 2006-2010.

Results: In the years 2006-2010 the UCU was given blood components for 23 645 hospitalized patients, more than half – men. The most common group of patients undergoing transfusion were patients aged 50 to 79 (69%). 188 125 blood components were issued. Red blood cell concentrate was the most commonly prescribed blood component.
Conclusions: The increase in pre-transfusion and transfused blood components was attributed to an increased number of hospitalized patients and an increased number of surgical procedures.
The use of IT systems in the management of blood enables you to monitor the demand for blood components, maximize their utilization and, consequently, effectively manage the blood components in the hospital blood bank.
The work, based on the largest database collected in Poland, is of an illustrative nature, in the future it will allow a detailed analysis of the use of blood components in particular hospital departments as well as comparison with other centers.
Are women with small endometriomas who undergo intracytoplasmic sperm injection at an elevated risk for adverse pregnancy, obstetric, and neonatal outcomes?
Authors: Verit Fatma Ferda , Kucukakca Ayse Seyma Ozsuer
Published: February 2021

Objective: The aim of the study was to investigate pregnancy, obstetric, and neonatal outcomes in women with small (<4 cm) unilateral endometriomas. Methods: This retrospective study included 177 patients: 91 patients with small endometriomas and 86 controls with unexplained or tubal factor infertility who were treated at the Süleymaniye Gynecology and Maternity Training and Research Hospital Infertility Unit between January 2010 and July 2015. The groups were matched with regards to demographic characteristics such as age, body mass index, and infertility duration. All of the women in this study conceived via intracytoplasmic sperm injection. We compared pregnancy, obstetric, and neonatal outcomes between these groups. Results: Women with endometriomas had a higher biochemical pregnancy rate, but lower clinical pregnancy and live birth rates than women with unexplained and tubal factor infertility (p<0.05 for all). However no significant differences were found in terms of obstetric and neonatal complications between the two groups (p>0.05 for all).Conclusion: In this study, we found that women with endometriomas less than 4 cm were more prone to early pregnancy complications. We also showed that this group did not have any increased risks of late pregnancy, obstetric, and neonatal complications.
Asigurari si reasigurari
Authors: Ionescu Roxana
Published: February 2021

Asigurarea exprima in principal o protectie financiara pentru pierderile suferite de oameni sau companii datorate unor diverse riscuri. Metodele de asigurare s-au dezvoltat o data cu nevoile de protective existente la un moment dat pentru oameni si afacerile acestora. Astfel oamenii de afaceri au inteles sa foloseasca asigurarea pentru a micsora riscurile ce pot aparea in tranzactiile comerciale si cele legate de protectia patrimoniului. Lucrarea se adreseaza, in primul rand, studentilor din invatamantul superior economic de stat si particular, elevilor din liceele cu profil economic, cadrelor didactice din invatamantul universitar si liceal, agentilor economici si institutiilor statului si oricaror alte persoane fizice sau juridice care doresc sa dobandeasca cunostinte in domeniul asigurarilor.
Data-driven insights into correlation among geophysical setting, topography and seafloor sediments in the Ross Sea, Antarctic
Authors: Lemenkova Polina
Published: February 2021

Detailed mapping based on the high-resolution grids, such as GEBCO, ETOPO1, GlobSed, EGM-2008 is crucial for various domains of Earth sciences: geophysics, glaciology, Quaternary, sedimentology, geology, environmental science, geomorphology, etc. The study presented a GMT-based scripting techniques of the cartographic data processing aimed at the comparative analysis of the bathymetry, sediment thickness, geologic objects and geophysical settings in the study area based on various datasets. The study area is located in the Ross Sea, Antarctic. The highest values of the sediment thickness over 7,500 m are dominating in the southwest segment of the Ross Sea closer to the Victoria Land, followed by the region over the Ross Ice Shelf with values between 5,500 to 7,000 m (170°-175°W). The increased sediment thickness (2,500 to 3,000 m) was also mapped seen in the region NE off the Sulzberger Bay (70-75°S to 140-155°W), caused by the closeness of the Marie Bird Land ice coasts. A remarkable correlation between the gravity and the topography of the sea-land border in the Marie Bird Land area is well reflected in the coastal line and a set of the higher values in the free-air gravity. On the contrary, negative values (–60 to -80 mGal) are notable along the submarine toughs stretching parallel in the western part of the basin: e.g. the trough stretching in NW-SE direction in the 170°W-175°E, 65°S-68°S, between the 167°W-175°W, 70°S-72°S. Such correlations are clearly visible on the map, indicating geological lineaments and bathymetric depressions correlating with gravity grids. The paper contributes to the regional studies of the Ross Sea, the Antarctic and Polar region, and development of the cartographic technical methodologies by presenting an application of the GMT for thematic mapping.
A semântica conceitual do campo
Authors: Melo William
Published: February 2021

Neste paper, os autores visam apresentar algumas aproximações, categorizações, contradições e complementaridades conceituais erigido nos últimos debates e ações a favor do desenvolvimento rural. Sendo assim, baseando-se na compreensão do território rural como uma esfera multidimensional e polissêmica, este paper abordará: 1) Idas e vindas do desenvolvimento rural nos ultimos trinta anos; 2) O polissêmico desenvolvimento do campo: a multiplicidade como questão; 3) Desenvolvimento urbano e rural: uma imbricada e complementar relação. Quanto ao método utilizado para o alcance das análises realizadas, foi empregada a análise de relatórios e documentos nacionais e internacionais sobre o tema, além de extensa revisão bibliográfica contemporânea datadas do inicio da década de 1990 até o dias de hoje.
Prefácio REIC - Edição Especial - Artigos do CTIC 2018
Authors: Nunes Ingrid
Published: February 2021

Esta edição especial da REIC reúne artigos que apresentam os trabalhos selecionados como os melhores trabalhos realizados por alunos de graduação submetidos ao 37º Concurso de Trabalhos de Iniciação Científica (CTIC) do XXXVIII Congresso da Sociedade Brasileira da Computação (CSBC), que ocorreu em Natal, Rio Grande do Norte, de 22 a 26 de julho de 2018.
Domain-Dependent Heuristics and Tie-Breakers: Topics in Automated Planning
Authors: Corrêa Augusto B. , G. Pereira André , Ritt Marcus
Published: February 2021

Automated planning is an important general problem solving technique in Artificial Intelligence. Given an initial state, a goal and a set of operators, we want to find a sequence of operators leading us to the goal. What makes planning interesting is that it can model different domains into planning tasks and solve them using a single method. In this work, we approach two different topics in planning. First, we study heuristics for the airport ground traffic problem and propose new heuristics that are better than any other known method. In the second part, we study tie-breakers for the A* search algorithm. We propose a new tie-breaking method that is proved to be the best possible and also show that our methods solve more instances than previous methods in literature
Avaliação de Soluções para Alocação de Aplicações Distribuı́das em Ambientes de Nuvem
Authors: Herrmann Ana Luiza , Galante Guilherme
Published: February 2021

Nos provedores públicos de IaaS, o usuário não possui informação precisa sobre a alocação de suas máquinas virtuais e como estão conectadas. Esse modelo de alocação pode levar a variações significativas na latência média entre as instâncias alocadas, o que pode resultar em degradação significativa do desempenho de aplicações distribuı́das, a menos que seja tomado cuidado em como os componentes da aplicação são mapeados para as instâncias.Neste trabalho avalia-se duas soluções, Cloudia e Choreo, que visam melhorar a alocação dos componentes de aplicações distribuı́das entre as VMs disponı́veis considerando essa questão da latência. De acordo com os experimentos ambas as soluções mostraram-se efetivas, reduzindo o tempo de execução das aplicações ao utilizar as alocações propostas.
ReonV: uma versão RISC-V do processador SPARC LEON3
Authors: Castro Lucas de Camargo Barros de , Azevedo Rodolfo Jardim
Published: February 2021

Este artigo reforça a importância de reutilização e contribuição para o desenvolvimento de hardware de código aberto, mostrando o exemplo de desenvolvimento de um processador soft-core RISC-V, dado o nome de ReonV, que foi desenvolvido reutilizando todos os módulos de um processador SPARC V8 de 32 bits já consolidado, modificando apenas seu pipeline para a nova ISA e herdando todos os outros módulos e o Board Support Package (BSP) do processador original.
Nota editorial
Authors: Dirección Comité
Published: February 2021

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Reflexión, objetivación, tematización: sobre una crítica heideggeriana de Husserl
Authors: León Felipe
Published: February 2021

De acuerdo con una influyente interpretación, ejemplificada por von Herrmann (2000), la postura que se asuma con respecto a la metodología reflexiva marca el contraste entre una vertiente reflexiva y una vertiente hermenéutica de la investigación fenomenológica. Recientemente, autores como Zahavi (2003a, 2005), Crowell (2001) y Cai (2011) han cuestionado la legitimidad de establecer dicho contraste a partir del método de la reflexión. Mi propósito es discutir la crítica central que Heidegger, basándose en Paul Natorp, dirige a la reflexión fenomenológica (GA 56/57, 100). Luego de presentar tres posibles interpretaciones de la crítica de Heidegger, y de sugerir la pertinencia de una de ellas, argumento que para contrarrestar dicha crítica es necesario ir más allá de la defensa de la metodología reflexiva que Husserl esboza en el § 79 de Ideas I, y apelar a una distinción entre una objetualidad cósica y una objetualidad temática, sugerida en textos husserlianos posteriores.According to an influential interpretation, epitomized by von Herrmann (2000), the stance that one takes on the reflective methodology determines a contrast between a reflective and a hermeneutic understanding of the phenomenological investigation. In recent years, authors like Zahavi (2003a, 2005), Crowell (2001), and Cai (2011) have challenged the validity of this interpretation. My aim in this article is to discuss the critique that Heidegger, drawing on ideas from Paul Natorp, addresses to phenomenological reflection (GA 56/57, 100). I first present three possible interpreta-tions of Heidegger’s critique, and suggest the superiority of one of them. Secondly, I argue that in order to respond to that critique one must go beyond the defense of reflection indicated by Husserl in § 79 of Ideas I, and appeal to a distinction between thing-objecthood and thematic objecthood, suggested in some of Husserl’s later writings.
Plutarco en España
Authors: Morales Ortiz Alicia
Published: February 2021

Este libro constituye un estudio de conjunto de las traducciones castellanas de Moralia de Plutarco realizadas en España durante el siglo XVI y aspira a ser una aportación más a nuestro conocimiento de la pervivencia del autor de Queronea en el Renacimiento hispano. Se incluye en esta investigación la versión que realizó Diego Gracián de Alderete (en las distintas ediciones aparecidas en 1533, 1548 y 1571), la más extensa de cuantas tenemos; además, la traducción llevada a cabo por Diego de Astudillo de los tratados De cohibenda ira y Coniugalia praecepta (1554); una versión aparecida anónimamente enValladolid en 1538 de la obra De cupiditatedivitiarum; la selección de los Apophthegmata debida a Pedro Simón Abril y dos traducciones más que se conservan manuscritas: la primera de ellas, del tratado De vitando aere alieno, debida a Juan Páez de Castro, nos ha llegado fragmentariamente, mientras que la segunda, del opúsculo De capienda ex inimicis util egado fragmentariamente, mientras que la segunda, del opúsculo De capienda ex inimicis utilitate, salió de la pluma del "maestro" Gaspar Hernández, personaje de identificación dudosa.
A multidisciplinary approach for the restoration of a crown-root fracture with the involvement of supracrestal attached tissues: a case report with 7 year follow up
Authors: CAN SAY Esra , DİKİCİ Burcu , ÇAKAR Gökser
Published: February 2021

Restoration of crown-root fractures with the involvement of supracrestal attached tissues represents difficulties for clinicians, as these types of fractures require a multidisciplinary approach for adequate treatment and successful prognosis. Depending on the location of the fracture, different treatment approaches, such as periodontal crown lengthening procedures, rapid orthodontic or surgical root extrusion or tooth extraction followed by fragment reattachment, direct composite restorations, veneers, and crown restorations, have been indicated.
This case report describes the management and long-term follow-up of the reattachment of a crown-root fracture using unidirectional fiber reinforcement after periodontal crown lengthening. Clinical and radiographic examinations of the reattached tooth after 7 years revealed favourable functional, physiological, and aesthetic outcomes and healthy surrounding periodontal structures, showing the success of the multidisciplinary treatment approach.
Dentists’ Knowledge about Management of Traumatic Dental Injuries in Turkey: A Cross-Sectional Study
Authors: CİFTCİ Volkan , SERİN Buse
Published: February 2021

Objective: The aim of the present study was to evaluate the knowledge level of general dental practitioners (GDPs) and dental specialists (DSs) about the management of TDIs.
Materials and methods: A total of 199 participants from three cities in Turkey completed the online questionnaires. The first section of the questionnaire consisted of questions regarding personal information, while the second section consisted of 12 multiple-choice questions regarding the knowledge of managing different types of TDIs in children. The data were statistically analyzed using the chi-square test and t-test.
Results: The questionnaires were completed by 145 GDPs and 54 DSs. In total, 29.1% of the participants (58) had less than 3 years of experience, 20.6% (41) had between 3 and 6 years of experience, 16.1% (32) had between 7 and 10 years of experience, 14.1% (28) had between 11 and 15 years of experience, 8% (16) had between 16 and 20 years of experience, and 12.1% (24) had more than 20 years of experience in clinical practice. Dentists with lesser years of experience (<10 years) were more knowledgeable than those with more years of experience (p=0.001). Moreover, DSs were more knowledgeable than GDPs (p=0.002).
Conclusion: Our findings revealed a moderate level of knowledge about TDI management among dental practitioners. These findings highlight the need to improve dentists’ knowledge about TDI treatment protocols.
On the structure of open equivariant topological conformal field theories
Authors: Fernàndez-València Ramsès
Published: February 2021

A classification of open equivariant topological conformal field theories in terms of Calabi-Yau $A_\infty $-categories endowed with a group action is presented.
Hypergroups and distance distributions of random walks on graphs
Authors: Endo Kenta , Mimura Ippei , Sawada Yusuke
Published: February 2021

Wildberger's construction enables us to obtain a hypergroup from a random walk on a special graph. We will give a probability theoretic interpretation to products on the hypergroup. The hypergroup can be identified with a commutative algebra whose basis is transition matrices. We will estimate the operator norm of such a transition matrix and clarify a relationship between their matrix products and random walks.
WHEN TWO PANDEMICS COLLIDE: RACISM, COVID-19 AND THE ASSOCIATION OF BLACK SOCIAL WORKERS EMERGENCY RESPONSE
Authors: Thomas Bernard Wanda
Published: February 2021

In the spring of 2020, African Nova Scotians were faced with two emerging pandemics: the ongoing pandemic of anti-Black racism, and the pandemic of COVID-19. The Association of Black Social Workers created a response specific to the needs of African Nova Scotians, employing the six practice principles of Africentric social work. They established a partnership with community and government partners to manage a phone line to triage based on need, and a virtual community check-in to connect about the pressing Black Lives Matter movement. This paper contextualizes the historic and current systemic racial inequities faced by African Nova Scotians within the context of the current public health emergency, and the need for an equitable, community-based emergency response. This specialized, Africentric service provision model can be used to inform the development of emergency responses for other Black communities in Canada.
«Hilado-Cordón-Cadena»: Symbolic Equivalence in La Celestina
Authors: Deyermond Alan D.
Published: February 2021

Not available.
La Celestina: documento bibliográfico. Cuarto suplemento
Authors: Snow Joseph T.
Published: February 2021

N/A
Índice analítico de autores, editores, directores y actores: LCBD. Suplementos I-V
Authors: Snow Joseph T.
Published: February 2021

N/A
La Celestina; o Traji-comedia de Calixto y Melibea
Authors: Snow Joseph T.
Published: February 2021

No disponible.
La Celestina: documento bibliográfico. Noveno suplemento
Authors: Snow Joseph T.
Published: February 2021

N/A
El concepto de la máscara en «La Celestina»
Authors: Garay René
Published: February 2021

No disponible.
Effects of training frequency on muscular strength for trained men under volume matched conditions
Authors: Johnsen Emil , van den Tillaar Roland
Published: February 2021

Background

In resistance training, the role of training frequency to increase maximal strength is often debated. However, the limited data available does not allow for clear training frequency “optimization” recommendations. The purpose of this study was to investigate the effects of training frequency on maximal muscular strength and rate of perceived exertion (RPE). The total weekly training volume was equally distributed between two and four sessions per muscle group.

Methods

Twenty-one experienced resistance-trained male subjects (height: 1.85 ± 0.06 m, body mass: 85.3 ± 12.3 kg, age: 27.6 ± 7.6 years) were tested prior to and after an 8-week training period in one-repetition maximum (1RM) barbell back squat and bench press. Subjects were randomly assigned to a SPLIT group (n = 10), in which there were two training sessions of squats and lower-body exercises and two training sessions of bench press and upper-body exercises, or a FULLBODY group (n = 11), in which four sessions with squats, bench press and supplementary exercises were conducted every session. In each session, the subjects rated their RPE after barbell back squat, bench press, and the full session.

Results

Both groups significantly increased 1RM strength in barbell back squat (SPLIT group: +13.25 kg; FULLBODY group: +14.31 kg) and bench press (SPLIT group: +7.75 kg; FULLBODY group: +8.86 kg) but training frequency did not affect this increase for squat (p = 0.640) or bench press (p = 0.431). Both groups showed a significant effect for time on RPE on all three measurements. The analyses showed only an interaction effect between groups on time for the RPE after the squat exercise (p = 0.002).

Conclusion

We conclude that there are no additional benefits of increasing the training frequency from two to four sessions under volume-equated conditions, but it could be favorable to spread the total training volume into several training bouts through the week to avoid potential increases in RPE, especially after the squat exercise.
Microbiomes of different ages in Rendzic Leptosols in the Crimean Peninsula
Authors: Kimeklis Anastasiia K. , Gladkov Grigory V. , Zverev Aleksei O. , Kichko Arina A. , Andronov Evgeny E. , Ergina Elena I. , Kostenko Igor V. , Abakumov Evgeny V.
Published: February 2021

Rendzic Leptosols are intrazonal soils formed on limestone bedrock. The specialty of these soils is that parent rock material is more influential in shaping soil characteristics than zonal factors such as climate, especially during soil formation. Unlike fast evolving Podzols due to their leaching regime, Leptosols do not undergo rapid development due to the nature of the limestone. Little is known how microbiome reflects this process, so we assessed microbiome composition of Rendzic Leptosols of different ages, arising from disruption and subsequent reclamation. The mountains and foothills that cover much of the Crimean Peninsula are ideal for this type of study, as the soils were formed on limestone and have been subjected to anthropogenic impacts through much of human history. Microbiomes of four soil sites forming a chronosequence, including different soil horizons, were studied using sequencing of 16S rRNA gene libraries and quantitative PCR. Dominant phyla for all soil sites were Actinobacteria, Proteobacteria, Acidobacteria, Bacteroidetes, Thaumarchaeota, Planctomycetes, Verrucomicrobia and Firmicutes. Alpha diversity was similar across sites and tended to be higher in topsoil. Beta diversity showed that microbiomes diverged according to the soil site and the soil horizon. The oldest and the youngest soils had the most similar microbiomes, which could have been caused by their geographic proximity. Oligotrophic bacteria from Chitinophagaceae, Blastocatellaceae and Rubrobacteriaceae dominated the microbiome of these soils. The microbiome of 700-year old soil was the most diverse. This soil was from the only study location with topsoil formed by plant litter, which provided additional nutrients and could have been the driving force of this differentiation. Consistent with this assumption, this soil was abundant in copiotrophic bacteria from Proteobacteria and Actinobacteria phyla. The microbiome of 50-year old Leptosol was more similar to the microbiome of benchmark soil than the microbiome of 700-year old soil, especially by weighted metrics. CCA analysis, in combination with PERMANOVA, linked differences in microbiomes to the joint change of all soil chemical parameters between soil horizons. Local factors, such as parent material and plant litter, more strongly influenced the microbiome composition in Rendzic Leptosols than soil age.
Evaluation of Nurses’ Workload in the Intensive Care Unit, Neonatal Intensive Care Unit and Coronary Care Unit: An Analytical Study
Authors: Azadi Maryam , Azimian Jalil , Mafi Maryam , Rashvand Farnoosh
Published: February 2021

Introduction: The workload on nurses can have adverse effects on the patient, nurse and healthcare system such as reduced quality of care, increased risk of nursing errors, reduced patient satisfaction, increased nurse anxiety, increased nursing job stress, increased risk of infection, increase in the length of hospital stay and increased risk of death. Aim: The present study was designed and conducted to compare nurses’ workload in the Intensive Care Unit (ICU), Neonatal Intensive Care Unit (NICU), and Coronary Care Units (CCU). Materials and Methods: The present study is a cross sectional analytical study that was conducted in the ICU, NICU and CCU of educational hospitals affiliated to Qazvin University of Medical Sciences. The convenience sampling method was used. A nursing activity score was used to assess nurses’ workload. The total score in this instrument is between zero and 178. Data were analysed using SPSS 16. Pearson correlation coefficient, chi-square, independent t-test, one-way analysis of variance was used. Results: The mean score of the total workload in nurses was 104.19±25.18. Regarding the primary purpose of the study, the results of the present study showed that the mean score of nurses’ workload was significantly higher in nurses working in the NICU than nurses working in the ICU and CCU (p<0.05). Among the demographic variables, only the marital status was significantly associated with nurses’ workload, that married nurses experienced more workload in some shifts (p<0.05). Conclusion: Nurses working in NICUs experienced a higher level of workload compared to the nurses in ICU and CCU. Due to the high workload of nurses in the NICU and the complications that this can cause for neonatal patients and nurses, it is necessary to pay more attention to the distribution of nurses in these wards.
Membranoproliferative Glomerulonephritis- A Disease or a Pattern of Injury?
Authors: Karmakar Subhrajyoti , Basu Keya , Sengupta Moumita , Roychowdhury Arpita , Pandey Rajendra , Bandopadhyay Manimoy
Published: February 2021

Introduction:Membranoproliferative Glomerulonephritis (MPGN) is a pattern of glomerular injury resulting from wide range of diseases sharing a common pathogenesis and thus, presented with large spectrum of clinical presentation starting from slowly progressive subnephrotic range proteinuria to nephritic syndrome and Rapidly Progressive Glomerulonephritis (RPGN). Aim: The aim of the study was to do reanalysis of the aetiological classification of MPGN and its prognostic outcomes. Materials and Methods: A retrospective, cohort study was conducted including the patients of all age groups with MPGN pattern of injury, from February, 2018 to August, 2018 at a Tertiary Healthcare Centre of West Bengal, India. All specimens were examined by two nephropathologists at the institute using both light microscope and immunofluorescence microscope. Haematoxylin and Eosin (H&E), Periodic Acid-Schiff (PAS), silver methanamine, Masson’s Trichrome (MT) stained smears were prepared for light microscopy. Specimens for immunofluorescence microscopy were stained using Fluorescein Isothiocyanate (FITC) conjugated polyclonal rabbit anti-sera against human IgG, IgM, IgA, C3, C1q, kappa, lambda. Correlations were evaluated using Spearman’s rank correlation. A p<0.05 was considered as statistically significant. GRAPHPAD PRISM 5 was used for statistical analysis. Results: From a total of 47 cases, 41 cases in immunoglobulin associated group, 4 cases in C3 nephropathy group and 2 cases in pauci-immune group were found. Autoimmune diseases had the strongest association followed by infections and dysproteinemia. Severity and chronicity parameters were more prevalent in the immunoglobulin positive group. The pauci-immune group showed maximum vascular involvement, indicating it to arise from a vascular pathology. Conclusion: Membranoproliferative Glomerulonephritis is not a single disease entity instead this pattern of injury could be seen in a variety of diseased condition.
Gastrointestinal and Extra-Gastrointestinal Stromal Tumours: A Clinicopathological and Immunohistochemical (c-KIT) Study
Authors: Yaranal Parasappa Joteppa , Prasanna N , Anand Anantharao Shankar
Published: February 2021

Introduction: Gastrointestinal Stromal Tumour (GIST) is one of the most common mesenchymal tumours of GIT. They have been proved to be arising from the smooth muscle pacemaker interstitial cells of Cajal, these cells are involved in gut motility and peristaltic movements. They can also rarely arise outside the Gastrointestinal Tract (GIT) from retroperitoneum, mesentery and omentum called as Extragastrointestinal Stromal Tumor (EGIST). Single best defining feature of GIST is positivity for Cytoplasmic Tyrosine Kinase (c-KIT). Aim: The aim of the study was to analyse clinicopathological features and c-KIT expression in both gastrointestinal and EGISTs. Materials and Methods: This was a retrospective observational (cohort) study; over a period of two years from January 2018 to December 2019, done at Navodaya Medical College and Hospital, Raichur, Karnataka, which comprised of 12 cases of GIST. All the cases were a resected specimens and thorough evaluation of clinical, imaging and histopathological studies were done and forwarded for immunohistochemistry for c-KIT expression. Results: Age of cases ranged from 04-70 years, mean age being 50.6 years and male to female ratio 2:1. Presenting symptoms of most of GIST were pain abdomen, diarrhea and few with vomiting whereas, rectosigmoid GISTs (2 cases and one with metastasis to liver) were associated with pain abdomen, bleeding per rectum and constipation. One case of retroperitoneal (extraintestinal) GIST was asymptomatic and other presented with pain in the right hip due to secondaries and one benign gastric serosal GIST was associated with gastric adenocarcinoma. The CT scan in two of malignant GISTs, confirmed metastasis and c-KIT study was negative in these 2 malignant GISTs. Conclusion: This study reaffirms importance of CD117 in diagnosis of GIST and EGIST, however, the negativity of CD117 does not rule out GIST, which requires thorough clinico-radiological and pathological correlation.
Malignant Gastric Neoplasms: A Clinicopathological Study with Special Reference to Serum CA125 Level with Peritoneal Dissemination
Authors: Mandal Ankita Pranab , Beso Adahra Patricia , Das Mou , Saha Rama
Published: February 2021

Introduction: Gastric cancer is a malignant tumour with a fifth position amid all cancers and its mortality rate is third globally. The most common distant mode of metastasis is Peritoneal Dissemination (PD). There is a positive correlation between serum Cancer Antigen 125 (CA125) level and PD in cases of malignant gastric neoplasm. Aim: To study the demographic features, the macroscopic and microscopic features of malignant gastric neoplasm and the relation of serum CA125 level with PD in cases of gastric cancer. Materials and Methods: This cross-sectional observational study was conducted for two years from January, 2018 to January, 2020 on 120 cases at Institute of Post Graduate Medical Education and Research, Kolkata, West Bengal (India). Detailed history taking, clinical examination, routine investigation was done. Peripheral blood sample for CA125 assay was collected. PD was identified through Ultrasonography (USG) or Computed Tomography (CT) or intraoperatively during open surgery. The gastrectomy specimens were sent for gross examination, staging and histopathology. Microsoft excel 2016 and SPSS 18 was used. Chi-square test was used for the comparison between the groups. A p-value of <0.05 was considered significant. The sensitivity, specificity, Positive Predictive Value (PPV), and Negative Predictive Value (NPV) were used for elective assessment. Results: This study comprised 120 cases with mean age of presentation as 54.3 years and male: female ratio of 2.3:1. Fifty cases (41.6%) were identified as tubular (solid)-poorly differentiated adenocarcinoma with 59 cases (49.2%) of stage IIIA and 52 cases (43.3%) of stage IV. The sensitivity and specificity of CA125 was 81.8% and 85.7%. The PPV and NPV were 56.2% and 95.4%. Conclusion: Early detection of peritoneal disease will help the clinicians to stratify the treatment of gastric cancers. Higher sensitivity suggests that preoperative serum CA125 is a promising tool to predict PD which aids in subsequent alternative mode of treatment, lesser morbidity and better survival of patients.
Histological Study of Placental Changes in Low Birth Weight Neonates in a Tertiary Care Hospital
Authors: Govindarajan Geethapriya , Ganapathy Shivashekar , Sudalaimuthu Muthu
Published: February 2021

Introduction: Low Birth Weight (LBW) in the newborn is a major health problem in our country and across the world. Identifying the pathological changes in placenta can help in understanding the pathogenesis of LBW babies. Aim: To compare histological changes in placenta of LBW baby and normal birth weight baby and to find out the maternal risk factors associated with LBW baby. Materials and Methods: A case-control study was done on a total of 140 samples which were collected in the study period of August 2017-August 2019 in SRM Medical College Hospital and Research Centre, Chennai, Tamil Nadu, India, with 70 placentas from LBW delivery and 70 placentas from normal birth weight delivery. Placentas from preterm delivery were excluded from this study. Histological changes in placenta were assessed in both the groups. Statistical analysis was done with Statistical Package for the Social Sciences (SPSS) version 17. Independent sample Student’s t-test and Chi-square test were used for comparison between two groups. Results: Histological changes like chorioamnionitis, syncitial knots, calcification, Perivillous Fibrin Deposition (PVFD), stromal fibrosis and basement membrane thickening were observed more frequently in placentas from LBW delivery. The commonest histological finding in present study was villous stromal fibrosis in 58 cases (82.9%) followed by excessive syncytial knots in 57 cases (81.4%). In this study, anaemia was the commonest maternal risk factor associated with LBW baby. Conclusion: The structure of placenta has strong relationship with the pregnancy outcome. There are few significant histological changes in placenta of LBW neonates. These changes have an impact in the development of the baby. Study about these changes provides a scope for prevention of certain morbidities in subsequent pregnancies.
Efficacy of Skin Microneedling in Combination with Narrow Band - Ultraviolet B Phototherapy in Vitiligo
Authors: Lotfy Sarah Sanad , Neinaa Yomna Mazid El-Hamd , Ghaly Nahla El-Sayed Ramzy , Doghaim Noha Nabil
Published: February 2021

Background: Vitiligo is a chronic cutaneous disease characterized by milky white depigmented patches that leave psychological impact on the patient's quality of life. New treatment modalities have been developed to shorten the duration of treatment of vitiligo with the least side effects.

Objective: To evaluate the safety & efficacy of microneedling in combination with narrow band-UVB in the treatment of vitiligo.

Patients and Methods: This study included 20 patients with stable vitiligo. They were treated by microneedling (one session every 2 weeks) in combination with narrow band-UVB (3 sessions weekly) for 3 months.

Results: The studied patients reported statistically significant degree of clinical improvements as follow;10%reportedgoodimprovement, 25% showe dmoderate improvement, 45% showed mild improvement and 20% showed noimprovement,after3months therapy. The reported side effects were minimal and transient in the form of minor pain, burning sensation and erythema at site of microneedling that disappeared spontaneously within few hours.

Conclusion: Microneedling in combination with narrow band-UVB phototherapy could be considered as effective treatment of vitiligo. Microneedling is a tolerable technique, harmless with negligible side effects.
Endoscopic Harmonic Midline Partial Glossectomy in Obstructive Sleep Apnea
Authors: Elkawa A. I. , Aglan Y. I. , Hagras M. A.
Published: February 2021

Aim: Our study was done to evaluate the role of Endoscopic posterior midline partial glossectomy as a surgical modality for the hypopharyngeal collapse in obstructive sleep apnea patients.

Study design: Prospective case series study.

Place and Duration of Study: Tanta university hospital, otolaryngology department, from October 2017 till March 2019.

Methodology: This was a prospective case series study, conducted on 10 patients from 2017 -2019 with tongue base collapse and normal craniofacial angles, the patients were evaluated preoperative and 6 months postoperative subjectively by Epworth sleepiness scale (ESS) and objectively by polysomnography and lateral cephalometry.

Results: Our study included 10 patients with age (mean ± SD48.70±4.08), BMI( mean ±SD24.45±1.56), 5 patients showed a significant reduction in AHI with a success rate of 50% with a significant change in ESS and the non-significant changes in cephalometric parameters.

Conclusion: Transoral endoscopic posterior midline partial glossectomy can improve the surgical outcomes of obstructive sleep apnea patients.
Phytochemical Screening and Antifungal Activity of Samadera indica
Authors: Jaiswal S. , Gupta R. A.
Published: February 2021

Samadera indica plant is an antifungal and bitter plant spread all over India. The applications of NDDS in phytopharmaceuticals have been widely investigated and various commercial formulations of plants are available in the global market. The goal of nano drug delivery system of herbal extract is to reduce toxicity, to improve drug efficacy, to improve better therapeutic effect, etc. Nanomicelles of samadera indica were successfully prepared by solvent evaporation method and studied various parameters such as Preliminary phytochemical screening, Scanning electron microscopy, and antifungal study. In present methodology, it was concluded that the Nanomicelles of methanolic extract of samadera indica plant was successfully prepared. It was safe and non- toxic. Plant extract contained flavonoids, triterpenes, and alkaloids which is responsible for antifungal activity. This research work concludes that the samadera indica is used as antioxidant, antimicrobial as well as antifungal compound. Novel drug delivery system is a advanced technology for delivering the drug with their so many advantages. Nanomicelles is an excellent pharmaceutical carriers by using bioactive constituents of plant having no side effects, effective formulation for various diseases especially in case of skin diseases. Novel formulation enhanced the topical drug delivery system of Samadera indica in order to treat skin infections was developed.
Evaluation of the Antihyperlipidemic and Antioxidant Effects of Catharanthus roseus Extracted from Vinca minor in Diabetic Rats
Authors: Aboelnaga Shimaa Mohammed Hasnin
Published: February 2021

The role of oxidative stress is known among the patients of diabetes as the level of reactive oxygen species was high among diabetic patients. This oxidative stress is generated in diabetic patients due to continuous high glucose levels that cause to decrease in the defence mechanism for antioxidant enzymes within the body. The reduction of the antioxidant defence mechanism leads towards the generation of hydroxyl radicals consequently results in lipid peroxidation. The objective of this study is to examine the efficacy of Vincamine extracts as antihyperlipidemic and antioxidant in diabetic rats. To evaluate the antihyperlipidemic and antioxidant effects of Vincamine, adult BB Wistar rats, weighing 150- 170 g were obtained and divided in six groups. Blood analysis was taken measure the observed parameters. The findings showed vincamine display antioxidant, hypoglycaemic and hypolipidemic activity.It is concluded that vincamine hasa protective role and acts as a good antioxidant activity along with effective antidiabetic effects.