bluuebunny/crossref_metadata_2025 huggingface dataset:
The Relationship of Health-Related Behavior and Chronic Diseases with Self-Rated Health, a Multinational Comparison among Adult Population: Evidence from EASS 2010 Health Survey
Authors: Majidul Islam Kishore Mohammad , Li Jiangping , Li Hongmin , Yang Huifang
Published: February 2022

Background: Multinational comparative study of the relation of self-rated health (SRH) with health behavior and chronic disease can ease our perception of differences in health factors between countries. We aimed to compare the relation of SRH with health behavior and chronic disease.
Methods: We attempted to analyze larger data obtained from 2010 East-Asian-Social-Survey and to compare the situation of China, Japan, South Korea, and Taiwan (Special administrative region of China). As these are neighboring regions having both similarities and differences in socio-demographic aspects as well as environment and cultures. The study was composed of 10,137 participants. Univariate and multiple regression analyses were employed to estimate the Odds Ratio (OR) and 95% Confidence intervals (CI) for SRH and health-related behavior, sociodemographic, chronic disease variables.
Results: The number of individuals reporting Good SRH was 6,648, with China having the higher proportion (P=<0.05). There were 3642 (35.9%) who suffered from chronic diseases. Overall, males, age group 18-30, nonreligious, higher education levels, singles, and those living in big cities had higher percentage of good health. Those without chronic diseases had higher percentage of good health (P=<0.05). The consumption of alcohol was associated with poor SRH in all regions. While less physical activity, diabetes mellitus, and ischemic heart disease were also responsible for poor SRH in all regions (P=<0.05).
Conclusion: Several health-related behaviors and chronic diseases are associated with poor self-rated health. Effective public health strategies, in promoting healthy lifestyles, and disease prevention intervention should be encouraged.
Twenty-Six-Year Trend of Mortality Rate due to Ischemic Heart Diseases (IHDs) in Iran: 1990-2015
Authors: Naderimagham Shohreh , Gohari Kimiya , Sheidaei Ali , Jamshidi Hamidreza , Namazi Shabestari Alireza , Modirian Mitra , Rezaei Nazila , Kompani Farzad , Damerchilu Bahman , Bahrami-Taghanaki Hamidreza , Larijani Bagher , Farzadfar Farshad
Published: February 2022

Background: Ischemic Heart Diseases (IHDs) are the main causes of deaths all over the world. Since there is no comprehensive study on IHDs mortality rate in Iran, the present study aimed to estimate age-standardized IHDs mortality rate by sex, age, geography, and time trends at both national and sub-national levels in Iran.
Methods: We used the Death Registration System (DRS) data from 1990 to 2015 collected by the Iranian Ministry of Health and Medical Education across the country, Tehran, and Isfahan main cemetery, not included in the DRS. Utilized death distribution methods to overcome the incompleteness of data. Statistical models including Spatio-temporal and Gaussian-Process Regression models were used to extrapolate all-cause and cause-specific mortality rates.
Results: Age-standardized IHDs mortality rate in Iran almost doubled from 1990 to 2015. Forty-nine deaths per 100.000 population in 1990, which increased to 91.6 deaths per 100.000 in 2015). Male to female age-standardized mortality rate increased from 1.07 to 1.32 during the studied period. Aging was associated with an increase in age-standardized IHDs mortality rate in both sexes, all provinces, and all of the years. The range of age-standardized IHDs mortality rate for both sexes was from 58 to 136.2 deaths per 100,000 across provinces in 2015.
Conclusion: Due to the increase in age-standardized IHDs mortality rate in Iran, it seems necessary to design and implement appropriate public health interventions by health authorities to prevent and control this group of diseases.
Liquid Consumption Conditions of Elderly People Living at Home and Investigation of Effecting Factors
Authors: Dönmez Buse , Demir Gokce
Published: February 2022

The article's abstract is not available.
Has COVID-19 Affected the Social Cohesion?
Authors: Shokri Azad , Moradi Ghobad , Mohamadi Bolbanabad Amjad , Moradpour Farhad , Younesi Fatemeh , Ebrazeh Ali
Published: February 2022

The article's abstract is not available.
Readiness-Based Implementation of Electronic Health Records: A Survey of Jordanian Nurses
Authors: Alsadi Mohammad , Saleh Ali , Khalil Malek , Oweidat Islam
Published: February 2022

Electronic health record (EHR) implementation is expanding worldwide to achieve the benefits of that technology, but it is reported in the literature as a “disruptive” change to the work environment in which all health-care workers need to be ready for the change, to enhance adoption and harvest the benefits. Jordan has rolled out a national EHR system. This study explored EHR implementation readiness, levels of realizing the benefits of EHR, and adoption among Jordanian nurses, using a self-report questionnaire at nine governmental hospitals in Jordan. A total of 462 registered nurses participated in the study. Results showed that nurses have moderate levels of readiness for EHR implementation, but higher levels of EHR benefits realization and adoption. All health-care workers ‘readiness for EHR implementation must be assessed regularly before, during, and after EHR implementation. Readiness-based roll-out can be used as a strategy in implementing EHR systems. Introducing a large-scale change management program is recommended to assess readiness, guide roll-out plans, enhance EHR implementation readiness, improve benefits realization, and increase EHR adoption levels, to help move health-care systems into the digital era.
Time Management Behaviors and Emotional Intelligence in Head Nurses in Emergency and Intensive Care Units
Authors: Tofighi Maryam , Tirgari Batool , Ghomian Zohreh , Safari Mehdi , Bazyar Jafar , Mohammadi Ehsan , Malekyan Leila , Safarpour Hamid
Published: February 2022

Background: Time management is of particular importance in nursing. One of the most effective variables associated with time management is emotional intelligence (EI). This study assessed the relationship between time management and EI and the level of EI and time management skills in head nurses in emergency and intensive care units. Methods: A cross-sectional study was conducted on all head nurses in the emergency and intensive care units of nine educational hospitals at Isfahan University of Medical Sciences in Iran in 2015 using Bradberry-Greaves ‘EI and Macan's Time Management Questionnaires. Results: Participants ‘total time management score was (104.15 ± 6.98); total EI score was (128 ± 15.80). There was no significant relationship between overall EI and time management skills. There was a significant relationship between age and the emotional self-awareness dimension of EI ( p = .027) and the mechanics dimension of time management ( p = .037), and between work experience and overall time management skills ( p = .049) and the mechanics dimension of time management ( p = .038). Conclusions: Specific EI and time management skills may help head nurses to cope with the challenges they face, which may improve the quality of nursing care. Nursing leaders should consider the importance of time management and EI in increasing motivation and satisfaction of nursing staff and improving quality of care.
Analisi Prformansi Teknologi CWDM (Coarse Wavelength Division Multiplexing) Pada Jaringan FTTH (Fiber To The HOme) Plasa Telkom Banjar Patroman Menggunakan Optisystem
Authors: Wahyudi Wildan Tri
Published: February 2022

Perkembangan teknologi telekomunikasi yang sangat pesat kebutuhan akan layananpun kian meningkat. Maka diperlukan jaringan yang dapat memberikan performansi yang lebih baik, saat ini penggunaan serat optik sebagai media transmisi digunakan pada Fiber to the home dan dipilih teknologi Coarse Wavelength Division Multiplexing yang merupakan teknologi transmisi serat optik yang mengombinasikan beberapa panjang gelombang yang berbeda dalam sebuah serat optik. Pada penulisan skripsi ini mengambil sampel di kota Banjar Patroman, sebagai kota kecil yang sedang berkembang dalam bidang telekomunikasi dimana media transmisi yang awalnya berupa kabel tembaga diganti menjadi kabel serat optik. Penelitian ini bertujuan untuk mengetahui kelayakan teknologi CWDM apabila diimplementasikan pada FTTH dimana dibuat simulasi dengan empat daerah di kota Banjar Patroman dengan jarak yang berbeda. Simulasi dilakukan dengan menggunakan software Optisystem, dan menghasilkan parameter kelayakan Link Power Budget, Rise Time Budget, dan Bit Error Rate (BER). Berdasarkan hasil simulasi menunjukan bahwa penggunaan teknologi CWDM pada FTTH menghasilkan daya terima sebesar -20,76 dBm dengan BER 2,9x10-14 pada jalur 1, sebesar -20,931 dBm dengan BER 7,43x10-13 pada jalur 2, sebesar -21,179 dBm dengan BER 1,09x10-11 pada jalur 3, dan sebesar -22,218 dBm dengan BER 2,14x10-8 pada jalur 4. Adapun hasil dari perhitungan diperoleh nilai daya terima sebesar -25,76 dBm pada jalur 1, sebesar -25,93 dBm pada jalur 2, sebesar -26,18 dBm pada jalur 3 dan sebesar -27 pada jalur 4. Hasil dari Rise Tme Budget sebesar 0,06708 ns untuk jalur 1, sebesar 0,0806 ns untuk jalur 2, sebesar 0,1007 ns untuk jalur 3, dan sebesar 0,1643 ns untuk jalur 4.
KETAHANAN PANGAN RUMAH TANGGA BERDASARKAN PENDAPATAN PETANI JAGUNG MANIS DI DESA BULUPOUNTU JAYA KECAMATAN SIGI BIROMARU
Authors: Vernando Veki , Jumiyati Sri . , Bachri Syaiful
Published: February 2022

Food is a basic need for humans to survive, and therefore adequate food for everyone is a basic right that deserves to be fulfilled. One of the important indicators affecting the food security status of farmer households is income. This study aims to determine the amount of income obtained from maize farming and the value of household food security of maize farmers in the Bulupountu Jaya Village, Sigi Biromaru District, Sigi Regency. The research was conducted from July to September 2020. The analytical method used is a descriptive based on the farm household income, staple food needs, and local staple food prices. The results showed that the income received by maize farmers for one planting season was IDR 6,384,691.85/farmer or IDR 5,010,159.76/ha with revenue of IDR 10,182,692.31/ farmer or IDR 13,441,153.85/ha. Meanwhile, the total production cost incurred is IDR 3,798,000.46/farmer or IDR 5,010,159.76/ha. The farm household income from outside maize farming comes from livestock businesses amounting to IDR 5,007,692.31 and as a laborer an amount of IDR 5,215,384.61, with a total side income of IDR 10,223,076.92. Farm household food security shows a value greater than 1 (Z>1), amounting to IDR 9,250,776.92, which means that maize farmer households with a side business will have a food security condition in a year and have an excess of IDR.9,250,776.92, to buy necessities other than staple food (rice).
MONITORING PROPOSAL OF THE EURASIAN WATER SHREW (NEOMYS FODIENS, PENNANT, 1771) IN POLAND
Authors:   Faculty of Animal Breeding, Bioengineering and Conservation, Warsaw University of Life Sciences – SGGW, Warszawa, Poland, GMAJ Michalina
Published: February 2022

The paper presents a proposal of methods for monitoring the Eurasian water shrew Neomys fodiens, belonging to the family Soricidae. This species has a wide range in Poland, although it has never been the subject of a nationwide survey. Water shrew has a sporadic and localised occurrence and little is known about its habitat requirements and population dynamics. Although there is no documented proof of population decline, there is concern that the species may be vulnerable to declines in abundance and occurrence as a result of habitat loss and pollution as it is closely associated with aquatic environment. The proposed method of population status assessment is based on estimating the number of specimen observed at selected monitoring sites by the capture-mark-recapture (CMR) method and an additional method of analysing owl pellets to establish the presence and abundance of the species in the area. Moreover, indicators assessing the condition of the habitat, such as presence of preferred water bodies, share of shoreline vegetation, slope of the shore and availability of shelters have been proposed. Regular monitoring can contribute to the recognition of potential changes in water shrew’s population dynamics.
MONITORING PROPOSAL OF THE SPECIES NEPTIS RIVULARIS (SCOPOLI, 1763) IN POLAND
Authors:   Faculty of Animal Breeding, Bioengineering and Conservation, Warsaw University of Life Sciences – SGGW, Warszawa, Poland, WNOROWSKA Kalina
Published: February 2022

Hungarian Glider (Neptis rivularis), a species of butterfly of the family Nymphalidae, which in Poland is under partial species protection and is included in the Red list of threatened animals in Poland with the EN category. The occurrence and abundance of Neptis rivularis are strictly dependent on the presence of Spiraea salicifolia L., which is the host plant of the species. Limiting the range of the occurrence of the Neptis rivularis in Poland also results from the decline in the number of suitable habitats. This article presents a proposed methodology for monitoring based on the evaluation of indicators of population condition in the form of: number of adults, abundance index, number of caterpillars and population isolation, and indicators of habitat condition – presence of the Spiraea salicifolia and exposure to the sun of microhabitats. The concept of the population condition assessment methodology was based on non-invasive observation of adults and counting caterpillars. The concept of habitat condition assessment methodology is based on a strong relationship between the Neptis rivularis and the Spiraea salicifolia. It is proposed to annually monitor the Neptis rivularis population in at least a dozen research areas within the country. This proposed monitoring methodology requires pilot studies to be carried out within Neptis rivularis occurrence sites in order to assess the effectiveness of the presented assumptions of the monitoring methodology for this species.
Diagnosis and Treatment of Scapular Fractures in Children and Adolescents
Authors: Naňka Ondřej , Bartoníček Jan , Havránek Petr
Published: February 2022

» Fractures of the growing scapula account for about 0.1% of all pediatric fractures, with the majority occurring at an age of ≥10 years.

» Radiographic diagnosis requires a detailed knowledge of the ossification sequence of the growing scapula. Computed tomography examination is indicated for displaced fractures of the glenoid fossa, the scapular neck, and complex fractures; magnetic resonance imaging should be performed in cases of suspected injury to the physis and with stress fractures, depending on patient age and the potential need for general anesthesia during the scanning process.

» Separation of the base of the coracoid process is often associated with acromioclavicular dislocation. Clavicular fractures rarely occur in combination with injuries to the growing scapula.

» The majority of scapular fractures can be treated nonoperatively. Indications for surgery are displaced intra-articular fractures, scapular neck fractures with a displacement of >2 cm, coracoid base separation associated with acromioclavicular dislocation, and scapulothoracic dissociation.

» Displaced intra-articular fractures of the glenoid fossa should be followed after healing until skeletal maturity. Complications are rare and occur most frequently with scapulothoracic dissociation.
Kopi Jahe Merah Khas Sumenep Madura di Desa Mulyoagung Jetis Kabupaten Malang
Authors: Nuriyanti Ika , Suhermin Ingsih Ita , Nikin Hardati Ratna
Published: February 2022

Home industry in Mulyoagung Village, Dukuh Jetis, Dau District, Malang Regency is engaged in micro business, namely The Making of Red Ginger Coffee. IRT "Yuli" has several problems, including: Red Ginger coffee production equipment is still simple, equipment has not been touched by science and technology, production results are not optimal, coffee sales income is not optimal, there is still a lack of understanding of hygienic and quality ginger coffee. There is a good seal and plastic to package the product, and there is also no proper economic analysis (marketing management) that is able to support the sale of ginger coffee products. Through this PKM, existing problems will be resolved by: Designing and making a ginger coffee grinder. Making a stamp tool for the ginger coffee brand. Providing training on bookkeeping and financial administration through POAC (Planning Organizing Actuating Controlling) concept. Providing training and mentoring to partners for hygienic ginger coffee making. The output targets are: 1) Manifesting a ginger coffee grinder tool so as to produce hygienic and clean ginger coffee; 2) Manifest the ginger coffee stamp as a packaging attraction for sales; 3) Providing training on management arrangements; and 4) Providing training for hygienic ginger coffee making.
TRANSFORMASI PERAN PUSTAKAWAN DI LEMBAGA PENELITIAN DAN AKADEMIK
Authors: Royani Yupi , Rahayu Rochani Nani
Published: February 2022

Undang-undang Nomor 43 Tahun 2007 tentang Perpustakaan, menjelaskan tentang profesi pustakawan beserta tugas dan tanggung jawabnya untuk melaksanakan pengelolaan dan pelayanan perpustakaan. Seiring dengan perkembangan teknologi informasi dan komunikasi, peran pustakawan mengalami pergeseran dari pelayanan konvensional ke arah digital. Suatu penelitian dengan metode literatur review dilakukan untuk menjelaskan pergeseran peran pustakawan di lembaga penelitian dan akademik dan kompetensi yang perlu dimiliki pustakawan di masa depan. Hasil yang diperoleh diharapkan dapat dimanfaatkan sebagai masukan kepada lembaga penelitian serta lembaga pendidikan terkait dengan bergesernya peran pustakawan. Berdasarkan hasil telaahan diungkapkan bahwa perubahan peran pustakawan di antaranya adalah sebagai informan riset, dosen/pengajar, penjaga informasi Super Highway, evaluator sumber daya digital, dan pengelola data penelitian. Ke depan, pustakawan harus memiliki kompetensi, di antaranya kompetensi dasar mencakup kemampuan bidang kepustakawanan, kemampuan teknologi informasi, kemampuan komunikasi, kepemimpinan, dan kemampuan inti meliputi pengetahuan subjek mandat institusi, kemampuan pengelolaan data koleksi, data penelitian, dan repositori, kemampuan terkait platform penyimpanan data, serta kemampuan pelestarian data digital.
Completion Designs in Horizontal Well with Transverse Hydraulic Fractures for Enhanced Recovery in Unconventional or Tight Reservoirs
Authors: Mishra Shubham , Fredd Christopher , Wilberg Dean , Yanbollu Umur
Published: February 2022

Low recovery, 2 to 15%, in unconventional plays (including tight reservoirs and source rocks) has long been recognized as a business deterrent. The industry applies enhanced oil recovery (EOR) techniques, along with hydraulic fractures in tight/unconventional plays, to improve the recovery. To maximize matrix sweep, the fractures are aligned in a face-to-face assembly. Such an arrangement can be achieved using a vertical or longitudinal hydraulic fracture on horizontal wells, but these, generally, do not provide as effective reservoir contact (hydraulic fracture surface area) as horizontal wells with multistage transverse hydraulic fractures. The multistage transverse hydraulic fracture, however, comes at the costs of conformance issues with early water breakthrough from short-circuiting and inability to achieve fracture face-to-fracture face alignment of the injection and production fractures. The vast majority of wells drilled in unconventional plays are in the transverse configuration; hence, there is a need for an optimal solution for transverse fractures combined with improved oil recovery (IOR)/EOR approaches.

In this work, we introduce the multistage enhanced recovery (MS-ER) techniques that enable face-to-face alignment for optimal enhanced hydrocarbon recovery (EHR)/IOR/EOR in horizontal wells with multistage transverse fractures, thereby enabling optimal recovery and mitigating the key risk of fracture short-circuiting.
Applications of Pozzolans to Treat Wellbore prior to Cement, Casing and While Drilling to Prevent Overburden Stress Fractures, Onshore US, Offshore Deepwater, and International Case Studies
Authors: Barry James Aloysius , Esatyana Erica , El Sayed Karim Loutfy , El-Husseiny Mahmoud Ahmed , Hagle John
Published: February 2022

This study is focused on the application of novel pozzolans (superplasticizers) ahead of cement, prior to casing and while drilling to treat and prevent wellbore fracture due to overburden stresses. This study is a combination of quantitative and qualitative analysis based on laboratory and field applications of pozzolanic materials in the construction of wells.

Pozzolans have long been applied to construction materials in order to improve lifespan and compressive strength. The application of pozzolanic materials goes back over 2000 years to the construction of Roman Aqueducts, buildings and roads known for their longevity and ability to resist corrosion and stress. These materials cover a broad range of naturally occurring and man-made materials. The most common pozzolanic materials used in drilling today include Bentonite, Kaolin, and Fly Ash. Pozzolans when combined with Portland Cement have been shown to increase the compressive strength and durability dramatically. Pozzolans are currently applied globally in cementing applications for HTHP, high loss zones and more.

This paper will examine the application in the drilling phase as an applied treatment during drilling for the reinforcement of the wellbore as well as to treat induced losses. The study will review cases for use of Novel Pozzolans for drilling, pre-cement, casing and for production zones the application of Novel acid soluble pozzolans for similar purposes. These applications validate the application of these materials beyond cementing into the drilling phase and wellbore construction for reducing backside pressure, reducing days on losses, reducing sidetrack, increasing operational ECD while drilling and cementing and achieving top of cement without inducing losses due to overburden in tight ECD window environments.

Deepwater environments provide a unique environment for the application of these novel materials as they offer some of the highest overburden environments with greatest operating costs and daily operating costs. Thusly this study has shown the applications have saved operators on multiple wells and over long periods many days and millions in operating costs with proven prevention of losses in field studies where wells were compared for offset value over a period of time. Multiple operators have adopted this technology as a result and there is a long track record of use while there are few papers on the subject. The purpose of this paper is to illustrate the best practices as well as new technologies and state of the art when it comes to the development of the latest in pozzolanics for these applications.
An Approximate Analytical Solution for Damage Radius Prediction in Water Injection Wells Based on Langmuirian Blocking: Derivation, Comparison and Field Applications
Authors: Liu Huifeng , Bedrikovetsky Pavel , Osipov Yuri V. , Yao Maotang , Xia Fuguo , Li Jiaxue , Li Jianbo
Published: February 2022

Water flooding is extensively used in the industry to develop the mature oilfields. However, after several years’ of injection, the injectivity usually declines and the injection pressure increases. This is because the solid or liquid particles in the injected water are retained in the pores and block the flow channel in the near wellbore region. The prediction of the particle retention and permeability damage is important for the design of damage removal methods like acidizing.

Previous researchers including M. Nunes, P. Bedrikovetsky, Feike J. Leij, et al. have done some work on the analytical solutions of the flow of particulate suspension in porous media with particle retention and consequent permeability reduction. However, they either assumed one-dimensional linear flow in the porous media or took the filtration coefficient as a constant. These are not always true because the flow of the injected water near the wellbore is radial and the filtration coefficient tends to decline with more and more particles being retained in the pores and ultimately reaches zero when all channels are blocked.

In this paper, we established a near-wellbore axisymmetric suspension flow and particle retention model based on Langmuirian blocking, obtained the approximate analytical solution and compared it with the numerical solution. The results showed that the error of our approximate solution for the retained particle concentration is within 5%. Then we incorporated the analytical expression into the Darcy's law equation and derived the expression of pressure drop as well as skin factor caused by particle retention. Damaged zone radius was also defined so as to estimate the acid volume needed to remove the damage. We also checked our models and solutions using field cases of water injection and acidizing from Tarim Oilfields, western China. The results showed that the injection pressure drop due to particle retention and the injection pressure recover after acidizing calculated from our models are basically consistent with the actual situation. Our models can be further used to predict the damage zone radius and design the acid volume for damage removal. The analytical solution can also be used to perform sensitivity analysis for the parameters involved.
RiskScape: A flexible multi-hazard risk modelling engine
Authors: Paulik Ryan , Horspool Nick , Woods Richard , Griffiths Nick , Beale Tim , Magill Christina , Wild Alec , Popovich Benjamin , Walbran Glenn , Garlick Russel
Published: February 2022

This paper presents the architecture and features of RiskScape software. RiskScape is an open-source software with a flexible modelling engine for multi-hazard risk analysis. The RiskScape engine implements modeller-defined risk quantification workflows as ‘model pipelines’. Model pipeline steps and functions analyse hazard, exposure, and vulnerability data across different spatio-temporal domains using geoprocessing and spatial sampling operations. The RiskScape engine supports deterministic and probabilistic risk quantification, with several probabilistic-based modes described in this paper. RiskScape advances modelling software for multi-hazard risk analysis through several implementation features. The RiskScape engine operates model pipelines independent of system prescribed model input data classifications or standards. Multiple hazard types, metric intensities, and temporal occurrence information is geometry processed and sampled to create coverage data of simultaneous or sequenced multi-hazard events at object-exposure locations. Escalating multi-hazard event impacts are then determined for object-exposures using scripted conditional or nested statements that apply vulnerability functions in a logical sequence of temporal hazard and impact occurrence. These model features, supported by open geospatial consortium standard geospatial data files and operations, expedite RiskScape for modelling multi-hazard risk at any geographical location or scale.
taxaTarget: Fast, sensitive, and precise classification of microeukaryotes in metagenomic data
Authors: Commichaux Seth , Javkar Kiran , Muralidharan Harihara , Ramachandran Padmini , Ottesen Andrea , Pop Mihai
Published: February 2022

BackgroundMicrobial eukaryotes are nearly ubiquitous in microbiomes on Earth and contribute to many integral ecological functions. Metagenomics is a proven tool for studying the microbial diversity, functions, and ecology of microbiomes, but has been underutilized for microeukaryotes due to the computational challenges they present. For taxonomic classification, the use of a eukaryotic marker gene database can improve the computational efficiency, precision and sensitivity. However, tools which use marker gene databases often implement universal thresholds for classification rather than dynamically learning the thresholds from the database structure, impacting the accuracy of the classification process.ResultsHere we introduce taxaTarget, a method for the taxonomic classification of microeukaryotes in metagenomic data. Using a database of eukaryotic marker genes and a supervised learning approach for training, we learned the discriminatory power and classification thresholds for each 20 amino acid region of each marker gene in our database. This approach provided improved sensitivity and precision compared to other approaches, with rapid runtimes and low memory usage. Additionally, taxaTarget was better able to detect the presence of multiple closely related species as well as species with no representative sequences in the database. Several algorithms were implemented, including threshold padding, which effectively handled the scarce training data available and reduced classification errors. Using taxaTarget on metagenomes from human fecal microbiomes, a broader range of genera were detected, including multiple parasites that the other tested tools missed.ConclusionData-driven methods for learning classification thresholds from the structure of an input database can provide granular information about the discriminatory power of the sequences and improve the sensitivity and precision of classification. These methods will help facilitate a more comprehensive analysis of metagenomic data and expand our knowledge about the diverse eukaryotes in microbial communities.
The Feasibility Of Telemedicine In Implementation Of Therapeutic Hypothermia For Management Of Neonatal Hypoxic-Ischemic Encephalopathy In Resource-Limited Areas.
Authors: Hadid Adnan , AL-Shantout Taher S. , Terkawi Rayan S. , Aldbes Baraa M. , Zahran Manal M. , Alsatouf Fadia A. , Najjar Hani , Mughrabieh MHD Hassan , Alhadid Nour A. , Altirkawi Khalid
Published: February 2022

Background: Telemedicine is widely used in neonatal services in developed countries. Lack of expertise and/or facilities, however, limited its use in developing countries and around areas of military conflicts. To our knowledge, no reports are demonstrating the feasibility of administering therapeutic hypothermia (TH) through telemedicine to neonates with hypoxic-ischemic encephalopathy (HIE) in resource-limited areas.Methodology: This is a retrospective study, evaluating 22 patients who received TH, guided by telemedicine, through a mobile app (Telegram®). We assessed the feasibility of utilizing Telemedicine in guiding the application of TH to infants affected with HIE in the North-West of Syria between July 2020 and July 2021.Results: Out of 5,545 newborn infants delivered during the study period, 22 patients were eligible for TH guided by Telemedicine. Patients were referred for consultation at a median (IQR) of 137 (35-165) minutes of life. A median (IQR) of 12 (3-18) minutes elapsed between the call for a consultation and the consultant response, and a median (IQR) of 30 (0-42) minutes elapsed between seeking the consultation and the initiation of cooling therapy. Eighteen patients completed cooling for 72 hours. The patients' temperatures were within the target range (33-34°C) most of the time (84.1%).Conclusion: Telemedicine is a feasible method to guide the implementation TH for HIE in resource-limited areas. The short-term success rate is relatively high; however, further studies with a larger population are needed to confirm these findings.
Dietary powder and molecular imprinted polymer nanoencapsulated sodium propionate to enhance growth performance, digestive enzymes activity, antioxidant defense and mucosal immune response in African cichlid (Labidochromis lividus) fingerlings
Authors: sarkheil mehrdad , Safari Omid , Kordestani Davood
Published: February 2022

This study was conducted to examine the effects of powder sodium propionate (P-SP) and SP loaded molecular imprinted polymer (MIP) nanoparticles (MIP-SP NPs) on growth, skin mucosal immune parameters and digestive and liver enzymes activities of African cichlid (Labidochromis lividus) fingerlings. The synthetized MIP-SP particles were characterized via FE-SEM/EDS, TEM, FTIR and ICP-OES techniques. Fish with an average weight of 500±2 mg were stocked into 12 experimental units and fed with experimental diets prepared by supplementation of basal diet (control) with MIP NPs, P-SP (5 g SP Kg−1 of dry diet) and MIP-SP NPs for 8 weeks. The findings demonstrated that growth performance parameters improved in the MIP-SP NPs followed by the P-SP dietary group compared to the control groups (P<0.05). The activity of digestive enzymes of lipase, trypsin, protease and alkaline phosphatase was higher in the fish fed with SP-supplemented diets than the controls (P<0.05). The highest activity of protease and lipase enzymes was observed in the MIP-SP NPs dietary group (P<0.05). The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels of liver tissue decreased in the SP dietary groups, while the catalase (CAT), superoxide dismutase (SOD) and alkaline phosphatase (ALP) levels increased in comparison to the control groups (P<0.05). The highest SOD and ALP levels were observed in the fish fed with the MIP-SP NPs-supplemented diet (P<0.05). The skin mucosal immune indices including alternative haemolytic complement activity (ACH50), lysozyme and total immunoglobulin (Ig) levels increased in the MIP-SP NPs and P-SP dietary groups in comparison to the controls (P<0.05). The findings indicated that sodium propionate encapsulated in molecular imprinted polymer nanoparticles could enhance the efficiency of dietary SP in African cichlid fish.
Text Feature Extraction of Myopia-related Information on Social Media —A Study Based on the Text Sentiment and Statistical Evidence
Authors: Zhang Zuyue , Jiang Mengyao , Rao Qingmao , Wang Haolin , Ye Chunlv , Cheng Ting , Hou Xiaorong
Published: February 2022

Background: In recent years, health information has become easily available on in social media. However, most of the available information lacks a persuasive effect. Texts providing information in health science on social media may affect how readers are convinced of the evidence presented. The purpose of this study is to examine health information on myopia prevention in WeChat using text sentiment indicators and to assess the extent of use of statistical evidence.Methods: A Web crawler is employed to collect health science information data from the WeChat official account. A total of 4,645 myopia prevention texts of children in WeChat were collected, and 813 valid texts were included in the study. A paired-samples T-test was used in the study. The text sentiment and use of statistical evidence of health science information were extracted by analysing the text features.Results: Results show higher use of positive words as compared to negative words (P < 0.01). Statistical evidence was used in over 96% of the text. Suggestions for the development of health science information in terms of text sentiment and statistical evidence are also proposed.Conclusions: This study investigated the emotional features of text and use features of statistical evidence of health science information in WeChat. The study found that producers of information related to myopia prevention prefer positive emotions and use statistical evidence in most information.
Lentivirus-Mediated Small Interfering RNA Targeting Piezo1 Inhibits Cartilage Matrix degeneration in Osteoarthritis Chondrocytes
Authors: Liu Yikai , Lu Xinzhe , Zhang Zian , Li Tao , Zhang Haining
Published: February 2022

Osteoarthritis (OA) is triggered by abnormal distraction stress, which eventually leads to cartilage degeneration. However, it remains unclear how the mechanical stress factor acts an effect on the destruction of the cartilage matrix. As a kind of mechanical sensitive ion channel protein, Piezo1 has the ability to sense and transmit mechanical signals to cells. Previous studies have found that the stress sensitivity of Piezo1 is closely related to the directionality of mechanical stress and the presence of extracellular matrix. This research aimed to explore the role of lentivirus-mediated Piezo1-siRNA transfection in extracellular matrix in chondrocytes of cultured osteoarthritis in vitro and to investigate the effect of Piezo1 on the progression of osteoarthritis. In this study, we demonstrated that the expression of Piezo1 mRNA in transfection group was significantly decreased after 24h mechanical stress was applied. However, the expression trend of collagen II and aggrecan was opposite to that of Piezo1, matrix metalloproteinase-13 (MMP-13), and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5). Our results indicate that Piezo1 could be used as a possible therapeutic target to attenuate the destruction of cartilage matrix induced by mechanical stress. Lentiviral-mediated Piezo1-siRNA can effectively interfere with the expression of Piezo1 and protect the expression of the extracellular cartilage matrix.
The influence of gastrectomy for gastric cancer on the spontaneous disappearance of Helicobacter pylori: A single-center prospective study
Authors: Yanagawa Senichiro , Fujikuni Nobuaki , Tanabe Kazuaki , Nakahara Masahiro , Noriyuki Toshio
Published: February 2022

Background: Helicobacter pylori (HP) eradication is recommended after endoscopic treatment of early gastric cancer (EGC). However, although cases of spontaneous resolution of HP after gastrectomy due to changes in the environment have been reported, there is no evidence for the efficacy of HP eradication in suppressing atypical carcinogenesis after gastrectomy. Herein, we prospectively studied the spontaneous disappearance rate of HP after gastrectomy in patients with gastric cancer.Methods: From April 2016 to May 2020, 100 patients who underwent gastrectomy with preoperatively confirmed HP in the stool antigen test were tested one year after surgery in this trial. There were 20 cases of total gastrectomy (TG), 9 cases of proximal gastrectomy (PG), and 71 cases of distal gastrectomy (DG) consisting of 29 cases with Billroth-1 (B-1) reconstruction and 42 cases with Roux-en-Y (RY) reconstruction.Results: After one year, 46 patients were positive, and 54 patients were negative for HP infection. In the univariate analysis, surgical procedure, pathological stage, and the presence of postoperative chemotherapy were significant factors for spontaneous resolution of HP infection. In multivariate analysis, only the surgical technique of TG remained as an independent factor. There were no significant differences in the rates of HP resolution between B-1 and RY reconstruction.Conclusions: One year after gastrectomy, spontaneous disappearance of HP was observed in approximately half of the patients. Therefore, HP eradication in patients with gastric cancer in the early postoperative period is not necessary, even if they are preoperatively positive for HP infection.Trial registration: Registration number: University Hospital Medical Information Network (UMIN) Clinical Trials Registry (UMIN ID: UMIN000046614); Registration date: 12/01/2022, retrospectively registered.
Clinical implication and immunological landscape analyses of ANLN in pan-cancer, a new target for cancer research
Authors: Zhang Lan , Wei Yong , He Yan , Wang Xiaping , Huang Zebo , Sun Libing , Chen Jie , Zhu Qingyi , Zhou Xin
Published: February 2022

Background
Actin-binding protein anillin (ANLN) is mainly involved in the process of cytokinesis and known to be dysregulated in diverse cancers. However, the role of ANLN in pan-cancer prognosis and tumor immunity remains unclear.
Methods
Gene expression profiles of 31 solid tumors were fetched from The Cancer Genome Atlas (TCGA) database. ANLN mRNA and protein expression were quantified via quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC). Protein expression of ANLN was further confirmed in Human Protein Atlas (HPA) database. Cox regression and Kaplan-Meier analysis were utilized to assess the prognostic value of ANLN in different tumors. The association between ANLN and different immune gene markers and infiltration cells were analyzed via ESTIMATE and CIBERSORT. A BLCA immunotherapy cohort: IMvigor (210) was utilized to confirm the role of ANLN in immune response.
Results
ANLN upregulation was detected in 21 types of cancers and was correlated with poor overall survival (OS), disease-free interval (DFI) and progression-free interval (PFI) in most cancers except in THYM. Additionally, correlation analysis revealed a significant association between ANLN expression and tumor mutation burden (TMB), microsatellite instability (MSI), immune cells infiltration and immune checkpoint genes in most cancer types. A BLCA immunotherapy cohort confirmed that patients with higher ANLN level had better immune responses and longer OS.
Conclusions
ANLN may serve as a prognostic biomarker for pan-cancer. ANLN upregulation is associated with higher TMB, MSI and immune cell infiltration in multiple types of tumors, shedding new light for cancer treatment.
Designing non-Hermitian real spectra
through electrostatics
Authors: Lee Ching Hua , Longhi Stefano , Li Linhu , Yang Russell , Tan Jun Wei , Tai Tommy Ying Zhe , Koh Jin Ming
Published: February 2022

Non-hermiticity presents a vast newly opened territory that harbors new physics and applications such as lasing and sensing. However, only non-Hermitian systems with real eigenenergies are
stable, and great efforts have been devoted in designing them through enforcing parity-time (PT) symmetry. In this work, we exploit a lesser-known dynamical mechanism for enforcing real spectra, and develop a comprehensive and versatile approach for designing new classes of parent Hamiltonians with real spectra. Our design approach is based on a novel electrostatics analogy for modified non-Hermitian bulk-boundary correspondence, where electrostatic charge corresponds to density of states and electric fields correspond to complex spectral flow. As such, Hamiltonians of any desired spectra and state localization profile can be reverse-engineered, particularly those without any guiding symmetry principles. By recasting the diagonalization of non-Hermitian Hamiltonians as a Poisson boundary value problem, our electrostatics analogy also transcends the gain/loss-induced compounding of floating-point errors in traditional numerical methods, thereby allowing access to far larger system sizes.
The Specific Inhibition of the Cardiac Electrogenic Sodium/Bicarbonate Cotransporter Leads to Cardiac Hypertrophy.
Authors: Mattía Romina Di , Diaz-Zegarra Leandro , Blanco Paula , Valverde Carlos , Gonano Luis , De Giusti Carolina Jaquenod , Portiansky Enrique , Vila-Petroff Martin , Aiello Ernesto , Orlowski Alejandro
Published: February 2022

Two alkalinizing mechanisms coexist in cardiac myocytes in order to maintain the intracellular pH: sodium/bicarbonate cotransporter (electroneutral isoform NBCn1 and electrogenic isoform NBCe1) and sodium/proton exchanger (NHE1). The dysfunction of these transporters has been previously reported as responsible for the development of cardiovascular diseases. The aim of this work was to evaluate the contribution of the downregulation of the NBCe1 to the development of cardiac hypertrophy. To specifically reduce NBCe1, we used shRNA cloned into a cardiotropic adeno-associated vector (AAV9-shNBCe1). After 28 days of being injected with AAV9-shNBCe1, the expression and the activity of NBCe1 in the rat heart were reduced. Strikingly, the downregulation of NBCe1 causes significant hypertrophic heart growth, lengthening of the action potential in isolated myocytes, increase in the duration of the QT interval and increase in the frequency of Ca2+ waves without any significant changes in Ca2+ transients. An increased compensatory expression of NBCn1 and NHE1 was also found. We conclude that the reduction of NBCe1 is sufficient to induce cardiac hypertrophy and modify electrical features of the rat heart.
Chronic necrotizing granulomatous skin lesions and MHC class I deficiency syndrome due to TAP2 deficiency
Authors: Darazam Ilad Alavi , Shahrooei Mohammad , Hakamifard Atousa , Olyaei Nasrin Alipour , Zerehpoosh Farahnaz Bidari , Gharehbagh Farid Javandoust , Hatami Firouze , Lotfollahi Legha , Mansouri Nahal , Casanova Jean-Laurent , Mansouri Davood
Published: February 2022

Major histocompatibility complexes class I (MHC- I) deficiency, also known as bare lymphocyte syndrome type 1 (BLS-1), is a rare autosomal recessively inherited immunodeficiency disorder with remarkable clinical and biological heterogeneity. Transporter associated with antigen processing (TAP) is a member of the ATP-binding cassette superfamily of transporters and consists of two subunits, TAP1 or TAP2. Any defect resulting from a mutation or deletion of these two subunits, adversely affects the peptide translocation in the endoplasmic reticulum, which is an important process for the proper assembly of MHC- I molecules. To date, few patients with reduced cell surface expression of MHC-I molecules have been reported. Herein; we described two Iranian cases with 2 and 3 decades delayed diagnosis of chronic necrotizing granulomatous skin lesions due to TAP2 deficiency without pulmonary involvement; and then segregation analysis in family members was performed by PCR and Sanger sequencing among 10 members of their family and found three homozygous mutation in three asymptomatic patients.
The trait coding rule in phenotype space
Authors: Wang Jianguo , He Xionglei
Published: February 2022

Is it possible to infer the genetically determined value of a quantitative trait from other traits? To answer the question we need to know how traits are coded in phenotype space (P) which can be partitioned into a subspace determined by genetic factors (PG) and a subspace affected by non-genetic factors (PNG). Evolutionary theory predicts PG is composed of limited dimensions while PNG may have infinite dimensions, which suggests a novel dimension decomposition method to separate them. After validating the method using simulation we applied it to a yeast phenotype space comprising 405 traits. The obtained yeast PG matches the actual genetic components of the yeast traits, explains the broad-sense heritability, and facilitates the mapping of quantitative trait loci, highlighting the success of the subspace separation. A limited number of latent dimensions in the PG were found to be recurrently used for coding the diverse yeast traits, while dimensions in the PNG tend to be trait specific and increase constantly with trait sampling. Similar results were obtained by analyzing the UK Biobank human brain phenome, which elucidated the genetic versus non-genetic origins of the left-right asymmetry of brain. In sum, phenotypic traits are coded by a rather small set of genetically determined basic dimensions and numerous trait-specific dimensions that are shaped by non-genetic factors, a rule enabling the identification of genetic components of quantitative traits based solely on phenotype.