bluuebunny/crossref_metadata_2025 huggingface dataset:
Pneumonia among under-five children in Ethiopia: a retrospective analysis from an urban hospital
Authors: Yallew Walelegn Worku , Assefa Selamawit , Yemane Berhane
Published: April 2023

Background: Pneumonia is the leading cause of death in under-five children in low-income countries. However, the burden of pneumonia in hospital admission is not traced systematically. This study was conducted to determine the proportion of under-five pneumonia admissions among children admitted to a hospital in Addis Ababa, Ethiopia between 2017-2021.
Methods: A retrospective record of pediatric admissions to the Yekatit 12 referral hospital in Addis Ababa, Ethiopia was assessed for the period 2017- 2021. The date of admission and discharge, length of stay, and outcome at discharge were collected in accordance with the Ethiopian National Classification of Diseases (NCoD). Descriptive statistics were used to assess the proportion of under-five children with pneumonia. Survival analyses using Log rank test and cox regression analysis were done to assess time to recovery (recovering from illness). Multivariable logistic regression was used to assess the influence of selected factors on pneumonia associated hospital admission.
Results: Between 2017-2021, 2170 children age 1 to 59 months were admitted, 564 (25.99%; 95% confidence interval 24.18% to 27.87%) were diagnosed with pneumonia. Among the sixty children who died during their hospitalization, 15 had been diagnosed with pneumonia. The median time to recover from pneumonia and discharge was 6 days. The odds of pneumonia hospital admission were higher among younger children (4.36 times higher compared to elder children with 95% CI 2.77,6.87)and were increased between the months of September to November.
Conclusions: Pneumonia accounts for more than a quarter of hospital admissions in under-five children and for a quarter of deaths in this urban cohort. Hospital admission due to pneumonia was higher among older children (36-59 months of age) in the months following the heavy rain months (September to November) as compared to younger children. Our data strongly support increase of vaccination to prevent under 5 pneumonia.
Gene rearrangements in the mitochondrial genome of Gonatopsis Borealis and Onychoteuthis Compacta reveal their phylogenetic implications for Oegopsida
Authors: Fan Fan , Jiang Lihua , Pei Liyi , Ye Yingying , Liu Yifan , Liu Bilin
Published: April 2023

Among cephalopods, Oegopsida is the largest and most diverse squid lineage. In order to further understand the taxonomy and evolution of the Oegopsida species, improve the molecular database of Oegopsida, and clarify the phylogenetic relationship between them, we obtained the mitochondrial genome sequences of Gonatopsis Borealis and Onychoteuthis Compacta. Their genome sizes were 20148bp and 20491bp, respectively. They all contain 13 protein-coding genes, 23 tRNAs and 2 rRNAs. We downloaded the latest Oegopsida genome sequence available on NCBI, and analyzed their 13 PCGs evolution rate. Apart from ND4, the rest of the genes were affected by Positive selection. Using two Loliginidae species as outgroups, the PCGs of 35 species were rearranged. The results showed that the gene rearrangements of the two species we obtained were in line with the characteristics of Oegopsida, and there were also gene rearrangements in species between families. Phylogenetic analysis was performed and it was found that the topology of the resulting tree was consistent with the morphological identification classification. These results allow for a better understanding of the phylogenetic relationships of the Oegopsida and enrich the data for the Teuthida.
Characterization of selected multidrug-resistant bacteria from clinical and hospital environmental sources using Vitek 2 compact system
Authors: Alabi Emmanuel Dayo , Bindawa Binta Lawal , Mzungu Ignatius , Adesoji Ayodele Timilehin
Published: April 2023

Background: Antibiotics resistant bacteria (ARB) are a global problem. Patients and hospital environments can be sources for dissemination of ARB that are Multi-drug Resistant (MDR).
Methods: We characterized MDR bacteria from clinical and hospital environments from selected hospitals within Katsina State, Nigeria. A total of 203 bacteria were isolated from 420 samples (clinical= 220 and hospital environment=200). Bacteria preliminary identification and antibiogram were determined by biochemical characterization and Kirby Bauer disk diffusion method, respectively. MDR bacteria were selected based on resistance to 3 classes of antibiotics. MDR bacteria were further identified and characterized with vitek-2- compact system. The micro-dilution automated system further identified Proteus mirabilis, Enterobacter cloacea spp dissolvens, Enterobacter cloacea and Pseudomonas aeruginosa as MDR isolates with the highest resistance phenotypes.
Results: Staphylococcus aureus was the most frequently isolated bacteria from clinical samples; i.e. infected surgical incisions (23.58%) and infected trauma wounds (20.75%) and hospital environmental samples; i.e. door handles (32.98%) and desks (14.43%). Highest resistance (92.79%) each to ampicillin and gentamycin was observed among hospital environmental isolates. Clinical isolates showed highest (80.19%) resistance to cefoxitin. MDR bacteria showed 12 antibiotics resistance patterns and most common (20/50) resistance phenotypes among MDR clinical isolates was to amoxiclav, cefoxitin and ciprofloxacin while resistance to ampicillin, chloramphenicol, colistin sulphate, kanamycin and nalidixic acid was commonly (10/50) observed among hospital environmental isolates.
Conclusions: High occurrence of MDR bacteria in the studied locations may be disseminated among patients, healthcare workers and the environment, and portend a great public health consequence. Hence, there is need for concerted AMR surveillance in these locations.
Complication of ruptured hepatic artery aneurysm in cardiac surgery under cardiopulmonary bypass: A case report
Authors: Lin Guoyun
Published: April 2023

Ruptured hepatic artery aneurysms (HAA) can lead to acute circulatory decompensation, which is usually fatal. Hence, HAAs must be identified and addressed rapidly to reduce the morbidity and mortality. Here, we report a 45-year-old female patient who was diagnosed with rheumatic heart disease, moderate mitral and aortic valve stenosis and regurgitation, and HAA. She underwent mitral and aortic valve replacements under cardiopulmonary bypass (CPB). At the end of the surgery, the patient’s blood pressure level plummeted, and abdominal distension was observed. An abdominal ultrasound suggested a ruptured HAA. After an emergency multidisciplinary consultation, selective hepatic arteriography through a right femoral artery approach and exploratory laparotomy were performed, which confirmed the diagnosis of ruptured HAA. After surgery, the patient was saved and stable. We reviewed the literature and found few reports of cases of heart valve surgery under CPB complicated by intraoperative HAA rupture. This case report shares our experience of anesthesia management in such cases.
MSDSC: A Multistage Deep Learning-based Skin Cancer Classifier
Authors: Hassan Afnan Mahmoud , Mahar Khaled , Fouad Mohamed Mostafa
Published: April 2023

One of the most frequent types of cancer in the globe is skin cancer which is a complicated public health issue. A sample of tissue from the skin lesion confirms the diagnosis of skin cancer. However, before making a definitive diagnosis, professionals detect specific signs that can be considered as early diagnosis. An early skin cancer diagnosis is prone to mistakes due to specialists’ lack of knowledge and similarities with other illnesses. This study presents a multistage deep learning-based skin cancer classifier (MSDSC) to give early detection of melanoma and non-melanoma skin lesions. The first stage is the pre-processing of samples to remove the hair surrounding the skin lesion. Since the dataset is unbalanced, the second stage was creating synthetic images using the Generative Adversarial Networks (GAN) model. This stage is followed by an attention-based U-Net model that gener- ates masks for regions of interest to eliminate the background. Lastly, two different types of EfficientNet are trained to classify skin lesions using segmented images. The proposed framework was tested using the International Skin Imaging Collaboration dataset (ISIC) and the results showed that it outperformed the state-of-the-art studies achieving an accuracy of 0.96, F1-score of 0.91, recall of 0.95, and precision of 0.88.
NUSAP1 is a Prognostic Biomarker and Correlated to Immune Infiltration in Lung Adenocarcinoma
Authors: Wang Yu-Sheng , Yin Ji-Zhong , Zhou Chao , Shi Xiao-Qian , Zhao Xue-Wei , Li Bing , Yang Meng-Hang
Published: April 2023

Lung adenocarcinoma (LUAD) is the most frequent subtype of lung cancer, the leading cancer-related death worldwide. Nucleolar spindle-associated protein 1 (NUSAP1) is a microtubule-associated protein firstly been found in proliferating cells, and recently known to be involved in carcinogenesis. However, the role of NUSAP1 in LUAD has yet to be explored. In current study, we used GEO datasets to identified a group of hub genes in LUAD, used Kaplan-Meier analysis to explore their impact on prognosis. NUSAP1 was chosen based on clinical relevance and literature searching results. The expression level of NUSAP1 was explored using TIMER, TCGA-LUAD database, human protein atlas (HPA), and verified by immunohistochemistry. Genes co-expressed with NUSAP1 were identified and GO/KEGG were performed to identify its biological functions. The impact of NUSAP1 on immune infiltration was investigated in TIMER. The correlation between NUSAP1 expression and clinical relevance were identified using diagnostic receiver operating characteristic (ROC) curves, nomogram model, and cox regression analysis. Immunohistochemistry was used to confirm the over-expression of NUSAP1 in LUAD and its correlation with MDSC. In short, we found a group of 20 hub genes in LUAD, all of which were differentially expressed in LUAD and most of them predicted a poor prognosis. NUSAP1 was upregulated in LUAD and most of the malignancies and correlated to multiple clinical parameters like pathological stage, N stage, M stage. Co-expressed genes and GO/KEGG showed that NUSAP1 mainly participates in cell cycle. Immune infiltration study demonstrated that NUSAP1 correlated to multiple immune cells, particularly myeloid-derived suppressor cell (MDSC). PrognoScan database verified the prognostic value of NUSAP1. Lastly, high expression of NUSAP1 and its correlation with MDSC was testified by immunohistochemistry. In summary, we found NUSAP1 were upregulated in LUAD and can serve as potential biomarker to indicate prognosis of LUAD.
LS-MolGen: Ligand-and-Structure Dual-driven Deep Reinforcement Learning for Target-specific Molecular Generation Improves Binding Affinity and Novelty
Authors: Li Song , Hu Chao , Ke Song , Yang Chenxing , Chen Jun , Xiong Yi , Liu Hao , Hong Liang
Published: April 2023

Molecule generative models based on deep learning have attracted significant attention in de novo drug design. However, most current generative approaches are either only ligand-based or only structure-based, which do not leverage the complementary knowledge from ligands and the structure of binding target. In this work, we proposed a new ligand and structure combined molecular generative model, LS-MolGen, that integrates representation learning, transfer learning, and reinforcement learning. Focus knowledge from transfer learning and special explore strategy in reinforcement learning enables LS-MolGen to generate novel and active molecules efficiently. The results of evaluation using EGFR and case study of inhibitor design for SARS-CoV-2 Mpro showed that LS-MolGen outperformed other state-of-the-art ligand-based or structure-based generative models and was capable of de novo designing promising compounds with novel scaffold and high binding affinity. Thus, we recommend that this proof-of-concept ligand-and-structure-based generative model will provide a promising new tool for target-specific molecular generation and drug design.
Comparative Study and Characterization of Water-treated Bacterial Cellulose produced by Solid or Liquid inoculum of Komagateibacter sucrofermentans
Authors: Drosos Athanasios , Kordopati Golfo G. , Anastasopoulos Charalampos , Zafeiropoulos John , Koutinas Athanasios A. , Kanellaki Maria
Published: April 2023

Structural and physicochemical properties of the water-treated bacterial cellulose (BC) which was produced by Komagateibacter sucrofermentans strain DSM 15973T either from a solid inoculum of the bacterial preculture in HS medium (BCS7) or liquid one (BCL7) after 7 days were investigated in this work. BCL7 was generally proved to be superior to BCS7 regarding BC yield and water holding capacity (WHC). BCL1-7 and BCS7 were analyzed by SEM (Scanning Electron Microscope), FT-IR (Fourier Transform Infrared Spectroscopy), XRD (X-ray diffraction), Nitrogen Adsorption-Desorption Isotherms, TG (thermogravimetric) and DTG (differential thermogravimetric) analysis, in order to investigate the structural modifications and physicomechanical properties of the water-treated BC pellicles. Both BC7 exhibited a high moisture content (98.8%) and the water release content for BCL7 of 1000 mL retained ~12 g of water after 100 h. BCL7 exhibited higher wet mass, specific surface area, pore volume and pore size distribution compared to BCS7. BCL showed an IV(a) isotherm and the crystallinity index of both BC7 were similar (about 55%). BC thermograms followed the same trend and BCS7 reveals a 35% residue up to 780oC. Overall, the resulted structural and physicomechanical differences between BCL and BCS pellicles appears that each could be used for different application.
Effect of Ar gas on the physical properties of Cadmium Sulfide diode prepared by PL deposition
Authors: Kadhim Suad M. , Dawood Yasmeen Z.
Published: April 2023

Cadmium sulfide (CdS) thin films were created using the pulse laser ablation technique and were applied to glass using varying pressure of argon gas (10, 20, and 30 torr). Characterized by X-ray diffraction and (scanning electron microscopy (SEM) and atomic force microscopy (AFM)) were studyed, additionally with various Ar gas pressures onelectrical characteristics are studied. The polycrystalline hexagonal structure of CdS films is demonstrated by X-ray diffraction analysis. To calculate the grain size of partical of deposited films which found the grain size ranges from (34-80) nm. Surface morphology measured using AFM give a spherical like structure and the average roughness was in the range of (6.45-11.49 nm).
The electrical measurement shows that the electrical conductivity of preoared films was from (5.9×10–4 ) to (13.8 × 10–4) (Ω cm)-1. From Hall measurements the deposited films show n-type conductivity. In order to make the CdS/Si junction a viable option for creating an effective photodiode.
A survey of Image Compression Algorithms based on Deep Learning
Authors: Wang Lichuan , Wang Shuchun
Published: April 2023

Images can carry more information than words, but the data space of images format is much larger than the text format when they are containing the same information. Therefore, how to efficiently compress images to improve their storability and transmissibility is one of the key research issues in the field of computer vision. Through consulting the relevant literature, this paper analyzes the development process of the current image compression technology, and introduces traditional compression methods and deep learning compression methods, while focusing on the compression methods based on deep learning. Through comparative experiments, this paper analyzes the performance of various types of neural networks in image compression tasks, and summarizes the advantages and disadvantages of various types of neural networks in compression tasks.
A Survey of Resources and Methods for Natural Language Processing of Serbian Language
Authors: Marovac Ulfeta , Avdić Aldina , Milošević Nikola
Published: April 2023

The Serbian language is a Slavic language spoken by over 12 million speakers and well understood by over 15 million people. In the area of natural language processing, it can be considered a low-resourced language. Also, Serbian is considered a high-inflectional language. The combination of many word inflections and low availability of language resources makes natural language processing of Serbian challenging. Nevertheless, over the past three decades, there have been a number of initiatives to develop resources and methods for natural language processing of Serbian, ranging from developing a corpus of free text from books and the internet, annotated corpora for classification and named entity recognition tasks to various methods and models performing these tasks. In this paper, we review the initiatives, resources, methods, and their availability.
The Data Analysis Remote Treatment Service (DARTS)
Authors: FARHI Emmanuel
Published: April 2023

We present the Data Analysis Remote Treatment Service (DARTS), an open-source remote desktop service that launches on-demand virtual machines in the cloud, and displays them in a browser. The environments can be used for e.g. scientific data treatment. DARTS can be deployed and configured within minutes on a server, and can run any virtual machine. The service is fully configurable, supports GPU allocation, is scalable and resilient within a farm of servers. DARTS is designed around simplicity and efficiency. It targets laboratories and facilities that wish to quickly deploy remote data analysis solutions without investing in complex hypervisor infrastructures.
Understanding the systematic implications of Pollinium morphology of Aeridinae (Orchidaceae)
Authors: Fan Jie , Li Chong-Chong , Jin Xiao-hua
Published: April 2023

Molecular phylogenetic studies have suggested major clades in the subtribe Aeridinae of Orchidaceae. However, systematic morphological charaters is still lacking. We carried out this study on the pollinium morphologies of subtribe Aeridinae with emphases on the aperture types and the pollinium sculpture to find out their systematic signification in light of a molecular phylogeny. The results indicate that most species have cleft type and few with porate type or none. All samples in this study have a complete tectum exine and most with psilate surface impling an advanced position of Aeridinae in Orchidaceae. Exine sculpture characters are consistent in each clades except Cleisostoma clade and Phalaenopsis clade. It has a somewhat clear changing tendency in consist with the molecular phylogeny with few exceptions. Gastrocohilus and Trichoglottis clades are supported by its non - flabellate pollinium, psilate bulgy sculpture, and irregular shape of individual units of pollinium. However, no distinguishing pollinium characteristics is found between them. Conclusionarily, exine sculpture characters are not systematic but useful characters in determining major systematic groups in subtribe Aeridinae.
Hybridization Domestication and Molecular Cytogenetic Characterization of New Germplasm of Thinopyrum intermedium with smGISH at Northeastern China
Authors: Liu Yizhuo , Song Weiwei , Song Anning , Wu Chunfei , Ding Jiarui , Yu Xiaoning , Song Jia , Liu Miaomiao , Yang Xinyuan , Jiang Changtong , Zhao Haibin , Li Xinling , Cui Lei , Li Hongjie , Zhang Yanming
Published: April 2023

Thinopyrum intermedium (2n = 6x = 42, StStJrJrJvsJvs), a perennial relative of wheat, is resistant or tolerant to biotic and abiotic stresses, making it an important genetic material for breeding perennial crops. It is also known as intermediate wheatgrass (IWG). The morphological performance, chromosome composition, and yield components of 24 newly developed perennial germplasm, designated 19HSC-Q and 20HSC-Z lines, were investigated from 2019 to 2022. Six lines of 19HSC-Q had higher values of yield component traits, such as thousand-kernel weight, grains per spike, and tiller number than that of Th. intermedium. 19HSC-Q14, 19HSC-Q18 and 19HSC-Q20 had the best agronomic traits with grain number per spike and thousand-kernel weight of 115, 97, and 102 and 7.674, 7.516, and 7.371 g in 2022, respectively. Three 20HSC-Z lines had been growing for two years that the grain number per spike was 116, 88, and 81, and the thousand-kernel weight was 7.146, 6.523, and 5.916 g, respectively. The results of sequential multicolor GISH analysis revealed that the Jvs-subgenome of Th. intermedium were divided into two karyotypes, type-I Jvs with 3 pairs of chromosomes and type-II Jvs with 4 pairs of chromosomes. Both IWG and 24 perennial germplasm had the similar chromosome composition but the translocation between subgenome chromosomes were detected in some lines with prominent comprehensive agronomic traits, such as 19HSC-Q6, 19HSC-Q11, 19HSC-Q18, and 20HSC-Z. The chromosome aberration could be distinguished into two types: the large fragment translocation with Jr-St, Jvs-St, Jr-IIJvs, and Jvs-Jr and the small fragment introgression of St-IJvs. Results of this study provide important materials for the next breeding cycle of cold resistant perennial wheatgrass.
Age and developmental stage specific Bioaccumulation of Copper during acute toxicity of Copper sulphate of two freshwater Fishes Rohu (Labeo rohita) and Mrigal (Cirrhinus mrigala)
Authors: Afridi Azam Jan , Zuberi Amina , Yousafzai Ali Muhammad , Zaidi Farrah
Published: April 2023

The widespread use of Copper resulted in chronic toxicity, triggering adverse health consequences for the aquatic organism. To investigate bioaccumulation of Copper, acute toxicity test / Median lethal concentration (LC50/96h)) of copper sulphate was performed at four aged group, and developmental stages viz fertilized egg to larvae, swim-up fry, advance fry, and fingerling of two freshwater fishes Rohu (Labeo rohita) and Mrigal (Cirrhinus mrigala). Each group was exposed to water born copper toxicity in selected glass aquaria. Resulted values of Median lethal concentrations (LC50/96h) of copper sulphate at Embryonic and larval, Swim-up fry, Advance fry, and Fingerling stages for Labeo rohita were 0.37, 0.75, 1.07, and 1.34 ppm and for Cirrhinus mrigala were 0.48, 0.94, 1.36, and 1.52 ppm respectively. Copper was analyzed in the whole bodies of first three developmental stages Viz; Embryonic and larval, Swim-up fry, Advance fry, while at fourth i-e at Fingerling stage five tissues Viz; gills, liver, kidney, skin, and muscle were selected. The resulted bioaccumulation order of copper at four developmental stages were Fingerling > Advance fry > Swim-up fry > Embryonic and larval stages for both species. At fourth, fingerling stage bioaccumulation of Copper was resulted in order of gills > liver > skin > kidney > muscle tissues for Rohu, and in order of liver > gills > kidney > skin > muscle tissues of Mrigal. This study provides baseline information for toleration of maximum quantity of Copper at four developmental stages. We recommend the pretreatment of industrial effluent before discharges into freshwater bodies.
Self and Mother Referential Processing in Phonological False Memory
Authors: Zhang Shuhui , Chen Zhu , Li Bingbing
Published: April 2023

It is well documented that self-referential processing enhances semantic false memory, yet the effect of self-referential processing on phonological false memory remains unclear. The current study used phonological DRM and self-reference paradigms to examine whether phonological false memory was affected by self and mother referential processing. In the current two experiments, we presented participants the Deese–Roediger–McDermott lists together with their own name, his/her mother’s name or “Lu Xun”. The results revealed that true recognition rates were not different between self-reference and mother-reference conditions and both were higher than the other-reference condition; there were no significant differences in false recognition rates among the self-reference, mother-reference and other-reference conditions. These results suggest that there are significant self-reference and mother-reference effects in true memory and no self-reference and mother-reference effects in phonological false memory. The findings are discussed in terms of theories of the self and mother reference effects and phonological false memory.
Metabolic syndrome and its associated factors among dyspeptic patients: A facility based cross-sectional study design
Authors: Nigatie Marye , Melak Tadele , Asmelash Daniel. , Alemayehu Ermiyas , Ashagrie Agenagnew , Worede Abebaw
Published: April 2023

Objective The aim of study is used to assess the association between Metabolic syndrome and its associated factors among dyspeptic patients at Tefera Hailu Memorial General Hospital, Sekota, North-East Ethiopia.Methods The study was a cross-sectional study design from March 01/2022 to May 30/2022. The study subjects were selected by convenient sampling technique. The data were entered into Epi. data 3.1 and analyzed using SPSS version 25. Logistic regression analysis was conducted and, P-value < 0.05 was used as a statistically significant variable.Results Our study included 228 study participants. The total prevalence of metabolic syndrome among study participants was 23.2%. Metabolic syndrome was statistically significantly associated with fasting blood glucose level(AOR: 15.965, 95% CI: 7.605–33.515), P < 0.001. The median serum level of low-density lipoprotein cholesterol (p < 0.001), triglyceride (P = 0.036), SBP(< 0.001and total cholesterol (P < 0.001) was a statistically significant difference between H. pylori positive and negative study participants.
Long non-coding RNA CASC2 inhibits malignant progression of endometrial cancer by negatively regulating mir-103a-3p/mir-107
Authors: Jia-ning Huo , Zi-hao Wang , Yi-bing Li , Yun-zheng Zhang , Xue-lian Chen , Xin Pan , Xiao-xin Ma
Published: April 2023

Background Long noncoding RNA CASC2 was first discovered as a tumor suppressor gene in endometrial cancer, but its mechanism is unclear. The purpose of this study was to investigate the effect of CASC2 on endometrial cancer and its mechanism.Methods We determined the expression of CASC2, miR-103a-3p/miR-107, PLAG1, and IGF2 in endometrial cancer and normal endometrial tissues through TCGA database and PCR analysis. It was subsequently confirmed that CASC2 and miR-103a-3p/miR-107 affect the biological behavior of endometrial cancer cells through a ceRNA mechanism. In addition, we confirmed this in vivo with a nude mouse xenograft model.Results The expression of CASC2 in endometrial cancer tissues is significantly downregulated, and this downregulation is related to tumor differentiation and affects tumor prognosis. Through cell experiments, it was found that CASC2 combined with miR-103a-3p/miR-107 can affect the biological behavior of endometrial cancer cells. Further research showed that CASC2, as the ceRNA of miR-103a-3p/miR-107, affected the expression of downstream target genes. The above cell experiments were also confirmed with in vivo experiments in a nude mouse xenograft model.Conclusions CASC2 combined with miR-103a-3p/miR-107 affects the malignant process of endometrial cancer. This study provides a new direction for the diagnosis and treatment of endometrial cancer.
Mir-302a-3p targets RUNX1 to inhibit the malignancy and epithelial-mesenchymal transition in endometrial cancer cells
Authors: Huo Jia-ning , Wang Zi-hao , Pan Xin , Li Yi-bing , Ma Xiao-xin
Published: April 2023

Background Runt related transcription factor 1 (RUNX1), a member of the RUNX family, is differentially expressed in various tumors. MicroRNA (Mir)-302a-3p, a member of the mir-302 family, is expressed at various levels in many tumors. We hypothesized that mir-302a-3p and its target RUNX1 jointly affected biological behavior of endometrial carcinoma.Method The binding sites of RUNX1 and mir-302a-3p were predicted by biological software and verified by luciferase assay. Real-time quantitative PCR was used to verify the expression of mir-302a-3p and RUNX1 at mRNA level. Western blotting was used to analyze the expression of RUNX1 and EMT-related proteins. The expression of RUNX1 was also validated by immunohistochemistry. The combined effect of Mir-302a-3p and RUNX1 on endometrial cancer was assessed in in vivo and in vitro models.Results RUNX1 was upregulated in endometrial cancer tissues, consistent with results from the TCGA database. RUNX1 knockdown can inhibit proliferation and promote apoptosis of endometrial cancer cells, as well as affect the G0-G1 phase of the cell cycle. Our previous results indicate that mir-302a-3p is down-regulated in endometrial cancer tissues. Bioinformatics analysis predicted a binding site between mir-302a-3p and RUNX1, which was validated by dual luciferase assays. We confirmed by quantitative real-time PCR and western blot that overexpression or knockdown of mir-302a-3p can affect the expression of RUNX1.Similarly, overexpression of mir-302a-3p inhibited proliferation, promoted apoptosis, and affected the G0-G1 phase of the cell cycle in endometrial cancer cells; these effects were more profound with co-transfection of mir-302a-3p agomir and shRUNX1.Further, mir-302a-3p and RUNX1 affected EMT in endometrial cancer, and the tumor-reducing effect of both RUNX1 knockdown and mir-302a-3p overexpression were confirmed in vivo.Conclusions Our results demonstrate that downregulation of mir-302a-3p promotes malignant biological behaviors and EMT in endometrial cancer cells by targeting RUNX1, and the miR-302a-3p/RUNX1 axis may be a potential therapeutic target for endometrial cancer.
Photon Blockade in the Fully Coupled Tripartite Hybrid System
Authors: Yan Shurui , Wang Yueming
Published: April 2023

We studied the fully coupled tripartite hybrid system with a two-level atom embodied in cavityopto-mechanical system. Applying the Shriever-Wolff approximation to the Hamiltonian of the sys-tem, we reached a fully diagonal effective Hamiltonian as well as its eigensystems. Thus the systemhas two turnable parameters including the detunings between the atom, cavity resonant frequencyand the driving laser field. We investigated the single- and two-photon blockade effects of the sys-tem under different detunings and cavity field driving strength using the probabilistic amplitudemethod and the quantum master equation method. The two-level atom plays an important role forthe photon blockade effect because it induces a shift of the cavity resonant frequency and a moreadjustable parameter for the system.
Model-Free Cooperative Optimal Output Regulation for Linear Discrete-Time Multi-Agent Systems Using Reinforcement Learning
Authors: Wu Beining , Wu Wei
Published: April 2023

In this paper, an off-policy model-free algorithm is presented for solving the cooperative optimal output regulation problem for linear discrete-time multi-agent systems. First, an adaptive distributed observer is designed for each follower to estimate the leader's information. Then, a distributed feedback-feedforward controller is developed for each follower to solve the cooperative optimal output regulation problem utilizing the follower's state information and the adaptive distributed observer. Based on reinforcement learning method, an adaptive algorithm is presented to find the optimal feedback gains via online data collecting from system trajectory. By designing a Sylvester map, the solution to the regulator equations is calculated via data collected from the optimal feedback gain design steps, and the feedforward control gain is found. Finally, an off-policy model-free algorithm is proposed to design the distributed feedback-feedforward controller for each follower to solve the cooperative optimal output regulation problem. A numerical example is given to verify the effectiveness of this proposed approach.
Numerical Study of High Temperature Heat Pipe to Suppress Gas Explosion
Authors: Gui Xiaohong , Wang Shengwei , Huang Junhui , Zhu Ziqiang , Zhao Chenyang
Published: April 2023

As a new type of heat transfer element, high temperature heat pipe has high heat transfer efficiency and broad prospects in suppressing gas explosion shock wave and flame. In this paper, the mechanism and effect of gas explosion suppression in high temperature heat pipe were analyzed by theoretical analysis and numerical simulation. ANAYS FLUENT was used to simulate the propagation process of gas explosion, and the simulation results were in good agreement with the experimental results. The influence of high temperature heat pipe on gas explosion propagation characteristics under different working conditions was studied. The results show that the flame propagation speed of gas explosion in the roadway with high temperature heat pipe is reduced, which will have nearly twice the time delay, the peak value of flame temperature is reduced, and the maximum overpressure of gas explosion in the roadway after the high temperature heat pipe is far lower than the maximum overpressure when the high temperature heat pipe is not laid. The layout of high temperature heat pipes in the roadway has different degrees of influence on the explosion suppression effect, and the best explosion suppression effect is achieved when the high temperature heat pipes are placed at 10 m intervals and inclined at 90°. The application of high temperature heat pipe technology to restrain gas explosion has certain feasibility, and the research results are of great significance to the safe and efficient production of coal mines.
The Usefulness of Humeral Intraosseous Infusion Performed by Rapid Response Vehicle Staff in Patients with Cardiac Arrest: A Single-Centre, Retrospective Study
Authors: Takamatsu Jumpei , Tsubota Hiroshi , Okajima Atsushi , Nakajima Hajime , Nakata Masatoshi , Kang Jinkoo , Yasue Yuichi
Published: April 2023

Background Humeral intraosseous infusion (IOI) is often performed in the prehospital setting by rapid response vehicle staff. IOI is used to administer extracellular fluid products and resuscitation drugs such as adrenaline to patients with trauma and cardiac arrest when venous channels are difficult to secure. However, in Japan, its usefulness is not well recognised, and it is rarely performed aggressively. This study aimed to demonstrate that humeral IOI is superior to the conventional peripheral venous infusion (VI) in cardiac arrest.Methods Among cases that received emergency care by rapid response vehicle staff from 2017 to 2022, 363 patients with cardiac arrest were transported to our institution with well-defined timing information. Patients were divided into humeral IOI and peripheral VI groups and compared in terms of age, sex, time from dispatch to contact and arrival at the hospital, time spent at the scene, distance travelled, type of injury, and return of spontaneous circulation (ROSC). Incident reports related to IOI during this period were also investigated. Continuous variables were compared by analyses of variance. Categorical data were compared using the chi-square test.Results There were no significant differences in terms of age, sex, time (min) from onset to dispatch (IOI: 3.19 ± 0.88, VI: 3.85 ± 0.60, P = 0.54), contact (IOI: 13.53 ± 0.96, VI: 14.11 ± 0.66, P = 0.62), and hospital arrival (IOI: 30.37 ± 1.15, VI: 30.51 ± 0.79, P = 0.92), time spent at the scene (IOI: 8.96 ± 0.35, VI: 8.25 ± 0.24, P = 0.1), or mileage (km) (IOI: 9.23 ± 0.46, VI: 9.58 ± 0.32, P = 0.53). There were significantly more cases of ROSC in the IOI group than in the VI group (IOI: yes 47: no 70, VI: yes 41: no 95, P = 0.001). There were no incident reports, such as unnoticed complications, continued infusions, and needle stick injury.Conclusions Humeral IOI required the same amount of time to perform as conventional peripheral VI but showed better outcomes in terms of ROSC. Humeral IOI is a valid infusion route until the completion of primary care. This simple and reliable procedure should be feasible for future prehospital emergency care.
The renaissance of the building stone in defining the architectural identity of the Jordanian city - (Amman city)
Authors: Shagin Musa , Awwad Bassam Abu , Safran Mansour
Published: April 2023

The material of building stone in this picturesque nature is a valuable treasure in the hands of man, and the value of building stone that man discovered since ancient times is because he believes that this material is the truth that preserves his artistic achievements over time and reflects the face of civilization and the thought of peoples, and man has invested in stone caves and in The manufacture of hunting and self-defense tools, and he used them in their basic function to bear the weights in building his dwellings, cities, and temples, and in the details also in the walls, ceilings, knots, doors, windows, stone columns, their bases, and muqarnas. And when we start today the stage of architectural design, we touch on the art of architecture and full knowledge of all the technical details in order to succeed in this work, and we read about the history of the architecture of civilizations and peoples for thousands of years and the stages of their development until our modern era, which reflected their religious and social philosophy and its continuous development, and it is a science that combines the past And the present with its primary components and has an extension and has no limits. It is the science of the building block over time that left us diligence to delve into this field, especially in our field of work after we left the academic period. The science of building stone reflects the civilized and cultural face of the peoples in general and the Arab peoples in particular.
Modular light-emitting diode shelving systems for scalable optogenetics
Authors: Gangemi Christina G. , Janovjak Harald
Published: April 2023

Optogenetic experiments rely on the controlled delivery of light to diverse biological systems. Impressive devices have been recently developed to simulate cells and small animals with multiple wavelengths and intensities. However, existing hardware solutions are often limited to a single sample holder and their design and cost can further limit scalability. This chapter describes an illumination system that is modular (through the use of accessible components) and scalable (through a shelving structure and low cost). Assembly and operation require no or minimal electrical engineering or programming expertise. Multi-intensity, wavelength and temporal experiments can be performed in dozens of small and large samples. This chapter also introduces methods for temperature and light intensity measurements towards appropriate illumination conditions. This work aims to provide a greater level of accessibility and complementary opportunities for large scale optogenetics in a broad range of biological samples.
Context-aware Multi-modal Recommendation Model based on Deep Auto-encoder
Authors: Gupta Rohan , Patel Arjun , Wilson Krik , Brown Kellen
Published: April 2023

With the exponential growth of data in the era of big data, recommendation systems have become essential in e-commerce. Accurate prediction of users' ratings of products is the key problem for personalized recommendation services. The current research in recommendation systems is mainly divided into three types: content-based, collaborative filtering, and hybrid recommendation. However, the traditional matrix decomposition algorithm faces difficulties in adapting to the current complex environment. In recent years, deep learning techniques have made great breakthroughs in recommender systems. We propose a multi-modal recommendation architecture based on self-encoder to effectively fuse textual and visual modal data, contextual features, and textual information to assist recommendation and significantly improve video recommendation performance. The proposed video recommendation model can effectively avoid the situation that the recommendation results cannot be obtained due to the lack of unimodal data. Specifically, the architecture comprises three key components: (1) a contextual feature extraction module based on convolutional neural networks to extract contextual information from textual data; (2) a multi-modal fusion module that fuses the contextual features with visual features to effectively integrate the complementary information across modalities; and (3) a self-encoder-based recommendation module that incorporates the fused features to generate personalized recommendations for users. Experimental results on several real datasets with distinctive features demonstrate the effectiveness of the proposed architecture. The proposed model significantly outperforms state-of-the-art recommendation models on the video recommendation task.
Determinants of late antenatal care attendance among high parity women in Uganda: analysis of the 2016 Uganda demographic and health survey
Authors: Tumwizere Godfrey , Mbonye Martin , Ndugga Patricia
Published: April 2023

Background Timely and adequate Antenatal Care (ANC) effectively prevents adverse pregnancy outcomes and is crucial for decreasing maternal and neonatal mortality. High-parity women (5 + children) are at higher risk of maternal mortality. Limited information on the late timing of ANC among this risky group continues to hamper Uganda's efforts to reduce maternal mortality ratios and improve infant and child survival. This study aimed to determine factors associated with attendance of the first ANC after 12 weeks of gestation among high-parity women in Uganda.Methods This study was based on nationally representative data from the 2016 Uganda Demographic and Health Survey. The study sample comprised 5266 women (aged 15–49) with five or more children. A complementary log-log regression model was used to identify factors associated with late ANC attendance among high-parity women in Uganda.Results Our findings showed that 73% of high parity women delayed seeking their first ANC visit. Late ANC attendance among high-parity women was associated with distance to the health facility, living with a partner, partner's education, delivery in a health facility, and Desire for more children. Women who did not find the distance to the health facility when going for medical help to be a big problem had increased odds of attending ANC late compared to women who found distance a big problem (OR = 1.113, CI: 1.004–1.234), women not living with partners(OR = 1.196, 95% CI = 1.045–1.370) having had last delivery in a health facility (OR = 0.812, 95% CI = 0.709–0.931), and women who desired to have another child (OR = 0.887, 95% CI = 0.793–0.993) had increased odds compared to their counterparts.Conclusions To increase mothers' timely attendance and improve maternal survival among high-parity women in Uganda, programs could promote and strengthen health facility delivery and integrate family planning with other services such as ANC and postnatal care education to enable women to seek antenatal care within the recommended first trimester. This study calls for increased support for programs for education, sensitization, and advocacy for health facility-based deliveries. This could be done through strengthened support for VHT and community engagement activities.
Ore composition and native gold characteristics of an ore occurrence in Eastern Transbaikalia
Authors: Nikanyuk T. S.
Published: April 2023

The article deals with the characteristics of the ores of an ore occurrence of the Munginsky cluster located on the western flank of one of the commercial fields of Eastern Transbaikalia. The ores of this promising ore occurrence are underexplored, especially in the technological aspect, which served the basis for studying their composition and characterizing native gold as their main commercial component. The composition of ores and altered rocks was studied by the following methods: atomic emission spectral quantitative analysis, X-ray fluorescence, as well as phase atomic absorption and atomic emission analysis with inductively coupled plasma ICP-AES. The mass fraction of total and organic carbon was determined in the Central Fire Assay Laboratory of JSC Pokrovsky Rudnik, Blagoveshchensk. The proportion of carbonate carbon dioxide was determined by the method of titrimetric determination of carbon dioxide. The content of gold was given according to the data of assay melting, the content of silver – according to the data of atomic absorption analysis. Petrographic and mineragraphic studies were carried out using an optical microscope Olympus BX-51. The mineral composition of the ore sample was also estimated based on the results of X-ray diffraction analysis. The quantitative mineral composition was studied on the crushed material of the original ore with a particle size of -2 mm using the microscopic study data of transparent and polished thin sections on a microscope Nikon Eclipse LV 100 POL. The content of iron hydroxides was determined from the weight difference after the sample was treated with a 10 % oxalic acid solution in a water bath. Comparison of the results obtained on the ore occurrence with the materials on the ore composition of the closest deposit located in 30 km demonstrates that on the one hand they are similar and uniform, and on the other hand they feature some differences. Their common parameters include a rather high degree of sulfide content of ores and similar chemical and mineral composition with the predominance of arsenopyrite, an increased role of bismuth as well as the predominance of fine gold and its similar morphological characteristics. This fact allows to consider the ore occurrence under investigation as an integral part of a single ore system of the closest deposit.
A Study on Food and Related Crafts for Wedding Procession of Noble Families in the 1800s: Focused on 『Jeungbosalyepyeonlam』 and 『Bongjeobyolam』
Authors:   Association for International Tea Culture, Choi Bae Young
Published: April 2023

The purpose of this study was to comprehensively examine 『Jeungbosalyepyeonlam』, which recorded the wedding procession of noble families in the 1800s, and 『Bongjeobyolam』, which recorded wedding food, and reconstruct food table setting and related crafts to identify cultural characteristics. The main results are as follows. First, the table setting for elderly guests accompanying the groom or the bride contained consideration to relieve fatigue and aid digestion. Second, the big table for the groom or the bride was sent to the in-laws' house, honoring the cooking skills and happiness of both families and indwelling the wisdom of learning each other's food customs and tastes. Third, the table settings for dinner and late-night snack on the first day of the bride and groom symbolized their harmony. Fourth, the bride's family promoted the groom's health by selecting good ingredients for an early breakfast, lunch or snack, and dinner table setting. Fifth, plum blossom tea placed on the groom's tea table could be the subject of an elegant conversation at the first meeting with the bride's relatives. Each of these table settings was also a cultural outcome in which crafts such as tableware, soban, oiled paper cover, and bojagi were harmonized. In conclusion, the table setting for the wedding procession of noble families in the 1800s has significance that can be used as a prototype of cultural content that connects the wedding ceremony, food, and crafts complexly.