bluuebunny/crossref_metadata_2025 huggingface dataset:
Advertising of unhealthy foods and beverages around primary and junior high schools in Ghana's most urbanized and populous region
Authors: Amevinya Gideon Senyo , Vandevijvere Stefanie , Kelly Bridget , Afagbedzi Seth Kwaku , Aryeetey Richmond , Adjei Akosua Pokua , Quarpong Wilhemina , Tandoh Akua , Nanema Silver , Agyemang Charles , Zotor Francis , Laar Matilda E. , Mensah Kobby , Laryea Dennis , Asiki Gershim , Holdsworth Michelle , Laar Amos
Published: September 2022

Introduction

The advertising of energy-dense, nutrient-poor foods and beverages is a common feature in obesogenic food environments. Such advertising, within and around settings where children live, learn, and play, negatively affects their food acquisition and consumption. We examined the extent and nature of food and beverage advertising around primary and junior high schools in Ghana's most populous and urbanized region, Greater Accra.

Materials and methods

Outdoor advertisements for foods and beverages within a 250 m road network distance of 200 randomly sampled schools were geocoded. For each food and beverage advertisement, information was collected on the setting, type, size, and number of product types featured in the advertisement. Promotional techniques (promotional characters and premium offers) used in advertisements were documented. Advertised foods and beverages were classified using the INFORMAS and NOVA food classification systems.

Results

A total of 5,887 advertisements were identified around the schools surveyed, 42% of which were for foods and beverages. Advertisements were most prevalent at food outlets (78% of all food advertisements), but also along roads and on non-food structures. Overall, 70% of food advertisements featured non-core/unhealthy products, while 12 and 14% had core/healthy and miscellaneous (including soup cubes, seasonings, and tea) products. About 4% of food advertisements had only a product/brand name or logo displayed. One out of two of the foods and beverages advertised were ultra-processed foods, 30% processed, 3% processed culinary ingredients, and 17% unprocessed or minimally processed foods. Sugar-sweetened beverages were the most advertised food product type (32%). Promotional characters were found on 14% of all food advertisements (most–69% were cartoons or manufacturer's characters), while 8% of all food advertisements had premium offers (including price discounts and gift/collectables).

Conclusions

There is an abundance of unhealthy food advertisements around primary and junior high schools in the Greater Accra Region. Policy actions such as restricting the promotion of unhealthy foods in children's settings are needed to protect pupils from such advertising practices.
Quality of Chinese government environmental health information disclosure during COVID-19 pandemic: Satisfaction survey on University students
Authors: An Ruikun , Wang Feng , Hou Yihan , Hideki Kitagawa
Published: September 2022

Government played a vital role during the COVID-19 pandemic by disclosing related environmental health information to the public. A satisfaction survey is often used to evaluate the public's satisfaction of the government's information disclosure while reflecting problems in the current disclosure system. As University students generally have better cognitive skills, they efficiently received related information during the pandemic, and therefore 717 questionnaires completed by University students were selected for this study. During the pandemic, the quality of the government's environmental health information disclosure system ranked at 13.89, marginally higher than average. Moreover, the timeliness and content adequacy of the disclosure system ranked at a level slightly above average. By adopting Hayes PROCESS Model 4 and 8, this study found that there is a direct impact of environmental health knowledge and environmental health awareness on satisfaction. Furthermore, University students' environmental health knowledge and awareness enhanced satisfaction through the mediating effect of self-reported environmental behavior. Finally, this study attempted to discover the conditions under which environmental health knowledge and awareness would have a greater direct and indirect influence on satisfaction, that is, the reverse moderating effect of household income level. In addition, this paper offers policy recommendations to enhance quality of government environmental health information disclosure system.
Cost-effectiveness of various immunization schedules with inactivated Sabin strain polio vaccine in Hangzhou, China
Authors: Xu Yuyang , Liu Yan , Wang Jun , Che Xinren , Du Jian , Zhang Xiaoping , Gu Wenwen , Zhang Xuechao , Jiang Wei
Published: September 2022

Background

It is necessary to select suitable inactivated poliovirus vaccine(IPV) and live, attenuated oral poliovirus vaccine (OPV) sequential immunization programs and configure the corresponding health resources. An economic evaluation was conducted on the sequential procedures of Sabin strain-based IPV (sIPV) and bivalent OPV (bOPV) with different doses to verify whether a cost-effectiveness target can be achieved. This study aimed to evaluate the cost-effectiveness of different sIPV immunization schedules, which would provide convincing evidence to further change the poliovirus vaccine (PV) immunization strategies in China.

Methods

Five strategies were included in this analysis. Based on Strategy 0(S0), the incremental cost (IC), incremental effect (IE), and incremental cost-effectiveness ratio (ICER) of the four different strategies (S1/S2/S3/S4) were calculated based on the perspective of the society. Seven cost items were included in this study. Results of field investigations and expert consultations were used to calculate these costs.

Results

The ICs of S1/S2/S3/S4 was Chinese Yuan (CNY) 30.77, 68.58, 103.82, and 219.82 million, respectively. The IE of vaccine-associated paralytic poliomyelitis (IEVAPP) cases of S1/S2/S3/S4 were 0.22, 0.22, 0.22, and 0.11, respectively, while the IE of disability-adjusted life-years (IEDALY) of S1/S2/S3/S4 were 8.98, 8.98, 8.98, and 4.49, respectively. The ICERVAPP of S1/S2/S3/S4 gradually increased to CNY 13.99, 31.17, 47.19, and 199.83 million/VAPP, respectively. The ICERDALY of S1/S2/S3/S4 also gradually increased to CNY 0.34, 0.76, 1.16, and 4.90 million/DALY, respectively.

Conclusion

ICERVAPP and ICERDALY were substantially higher for S3 (four-sIPV) and S4 (replacement of self-funded sIPV based on one-sIPV-three-bOPV). Two-sIPV-two-bOPV had a cost-effectiveness advantage, whereas S2/S3/S4 had no cost-effectiveness advantage.
Influencing factors and mechanism of high-speed railway passenger overall comfort: Insights from source functional brain network and subjective report
Authors: Fan Chaojie , Lin Yating , Lin Shuxiang , Li Yingli , Wu Fan , Xiong Xiaohui , Zhou Wei , Zhou Dan , Peng Yong
Published: September 2022

Overall comfort is the priority for the high-speed railway (HSR) passengers, while its influencing factors and mechanism are not yet apparent. According to the source functional brain network and subjective report, this study revealed the potential influencing factors and mechanisms of passengers overall comfort in high-speed railway environments. Here, an ergonomics field test with 20 subjects was conducted where subjective reports and electroencephalography (EEG) were collected. The electric-source imaging and functional connectivity were used to build the source functional brain network from EEG and network indices were extracted. Statistics analysis results showed that static comfort played the most critical role in the overall comfort, followed by emotional valence, emotional arousal, aural pressure comfort, vibration comfort, and noise comfort. Thermal and visual comfort were insignificant due to the well-designed heating, ventilation, and air conditioning (HVAC) and lighting system of HSR. In addition, the source functional brain network of passengers who felt uncomfortable had the higher clustering coefficient, assortativity coefficient and global efficiency, which meant greater activation of brain compared with passengers who were in a state of comfort. According to the local attributes indices analysis, most key brain regions were located in the frontal and hippocampus, which revealed emotion and spatial perception contribute to the whole comfort degradation process. This work proposed novel insights into HSR passengers overall comfort according to subjective and objective methods. Our findings demonstrate emotional regulation and seat improvements are key factors for future improvement of HSR passengers overall comfort.
Evaluation of carboxylic acids as sustainable compatibilizing agents on blend sheets properties based on thermoplastic babassu starch and polypropylene
Authors: Maniglia Bianca Chieregato , Vedove Thaís Maria Aimola Ronca Dale , Tadini Carmen Cecília
Published: September 2022

Polypropylene/babassu thermoplastic starch (PP/TPS, 70:30 w/w) blend sheets were prepared as an alternative material to use in disposable packaging, reducing the negative environmental impact of synthetic polymer. Three different carboxyl acids: palmitic (PP/TPS/P), citric (PP/TPS/C), and stearic (PP/TPS/S), were used as natural compatibilizing agents (NCA) and compared to the conventional synthetic maleic anhydride (PP/TPS/M), concerning mechanical, physical, crystallinity, and morphological properties of the blend sheets. The blend sheets without or with compatibilizing agents resulted in dark coloration, higher opacity, higher thickness, lower resistance to break, flexibility and rigidity, higher moisture, solubility in water, hydrophilicity, lower crystallinity, and more heterogeneous morphology in comparison to PP sheets. The presence of compatibilizing agents was observed to improve the mechanical and physical properties of the blend sheets, resulting in materials stronger and less hydrophilic. The citric acid was able to act similarly to the synthetic maleic anhydride, resulting in blend sheets with similar mechanical and physical properties. Finally, this work shows that the natural compatibilizing agent citric acid was analogous to the commercial one, synthetic maleic anhydride, bringing a more sustainable alternative. In addition, this work allowed assessing a new source of starch and offering one more alternative for applying this source to the plastic packaging sector.
The Microbiome in Periodontitis and Diabetes
Authors: Silva Davi Neto de Araújo , Casarin Maísa , Monajemzadeh Sepehr , Bezerra Beatriz de Brito , Lux Renate , Pirih Flavia Q.
Published: September 2022

Objectives

To perform a comprehensive and integrative review of the available literature on the potential changes in the microbiome of healthy and individuals with diabetes under periodontal health and disease.

Materials and Methods

The review was conducted by two independent reviewers. Indexed electronic databases (PubMed/Medline, Cochrane Library, Web of Science and Scopus) were searched, including articles published in English and dated from 5 years ago until December 2021. A manual search also was performed to identify co-related articles. Following the removal of duplicates and eligibility criteria, the articles were included in tables for analysis and described in the manuscript.

Results

According to this review, diabetes mellitus was associated with significant changes in the subgingival and salivary microbiome, either in its association with periodontitis or in cases of periodontal health. In addition to affecting microbial diversity in terms of taxonomy, metagenomic studies have shown that this endocrine disorder may also be directly related to increased pathogenicity in the oral microbiome.

Conclusion

Although the reviewed studies demonstrate important differences in the subgingival and salivary microbiome composition because of diabetes mellitus, further studies are needed to clarify the real effects of hyperglycemia on oral microbial profiles and support new diagnostic approaches and innovative treatments.
The role of endoscopic management and adjuvant topical therapy for upper tract urothelial cancer
Authors: Chien Austin L. , Chua Kevin J. , Doppalapudi Sai Krishnaraya , Ghodoussipour Saum
Published: September 2022

Upper tract urothelial carcinoma (UTUC) has traditionally been managed with radical nephroureterectomy, and while this approach remains the gold standard for high-risk disease, endoscopic, kidney-sparing management has increasingly been adopted for low-risk disease as it preserves kidney function without compromising oncologic outcomes. Ureteroscopy and percutaneous renal access not only provide diagnoses by tumor visualization and biopsy, but also enable treatment with electrocautery or laser ablation. Several modalities exist for laser ablative treatments including thulium:YAG, neodymium:YAG, holmium:YAG, and combinations of the preceding. Furthermore, due to high recurrence rates after endoscopic management, adjuvant intracavitary instillation of various agents such as mitomycin C and bacillus Calmette-Guerin have been used given benefits seen in non-muscle invasive urothelial bladder cancer. Other formulations also being studied include gemcitabine, anthracyclines, and immunotherapies. More recently, Jelmyto, a mitomycin reverse thermal gel, has been developed to allow for adequate drug delivery time and potency since urine flow could otherwise dilute and washout topical therapy. In this article, the authors review techniques, indications, best practices, and areas of current investigation in endoscopic management and adjuvant topical therapy for UTUC.
Digitally Savvy at the Home Office: Computer Skills of Older Workers During the COVID-19 Pandemic Across Europe
Authors: König Ronny , Seifert Alexander
Published: September 2022

Digital skills can be a valuable resource in work life, especially in such times as the current COVID-19 pandemic, during which working from home has become new reality. Although increasing numbers of older employees (aged 50 years and above) are using digital technologies to work remotely, many of these older adults still have generally lower digital skills. Whether the pandemic will be a push factor for the acquisition of computer skills in late working life remains unclear. This study investigated the explanatory factors of the computer skills gained by older workers who were working from home during the COVID-19 pandemic, using representative data for 28 countries from the Survey of Health, Aging and Retirement in Europe (SHARE). The analysis of the survey responses of 11,042 employed persons aged 50 years and older revealed that, 13% worked only at home due to the pandemic, while 15% said they worked at home and in their usual workplace. The descriptives indicate that full-time homeworking is more of an option among those with tertiary education and who already have some computer skills. Of the older employees who worked only at home, 36% reported an improvement in their computer skills, whereas of the older workers who worked at home and at their usual workplaces, only 29% reported such an improvement. Our results based on logistic regressions suggest that significantly more women, younger employees, respondents with tertiary educational qualifications, and those whose work was not affected by unemployment or even business closure acquired new computer skills, regardless of whether they were working permanently or only partly from home. The study underlines the importance of investigating the possible digital skills gained from the home office situation resulting from the pandemic.
An event of extreme relativistic and ultra-relativistic electron enhancements following the arrival of consecutive corotating interaction regions: Coordinated observations by Van Allen Probes, Arase, THEMIS and Galileo satellites
Authors: Nasi Afroditi , Katsavrias Christos , Daglis Ioannis A. , Sandberg Ingmar , Aminalragia-Giamini Sigiava , Li Wen , Miyoshi Yoshizumi , Evans Hugh , Mitani Takefumi , Matsuoka Ayako , Shinohara Iku , Takashima Takeshi , Hori Tomoaki , Balasis Georgios
Published: September 2022

During July to October of 2019, a sequence of isolated Corotating Interaction Regions (CIRs) impacted the magnetosphere, for four consecutive solar rotations, without any interposed Interplanetary Coronal Mass Ejections. Even though the series of CIRs resulted in relatively weak geomagnetic storms, the net effect of the outer radiation belt during each disturbance was different, depending on the electron energy. During the August-September CIR group, significant multi-MeV electron enhancements occurred, up to ultra-relativistic energies of 9.9 MeV in the heart of the outer Van Allen radiation belt. These characteristics deemed this time period a fine case for studying the different electron acceleration mechanisms. In order to do this, we exploited coordinated data from the Van Allen Probes, the Time History of Events and Macroscale Interactions during Substorms Mission (THEMIS), Arase and Galileo satellites, covering seed, relativistic and ultra-relativistic electron populations, investigating their Phase Space Density (PSD) profile dependence on the values of the second adiabatic invariant K, ranging from near-equatorial to off equatorial mirroring populations. Our results indicate that different acceleration mechanisms took place for different electron energies. The PSD profiles were dependent not only on the μ value, but also on the K value, with higher K values corresponding to more pronounced local acceleration by chorus waves. The 9.9 MeV electrons were enhanced prior to the 7.7 MeV, indicating that different mechanisms took effect on different populations. Finally, all ultra-relativistic enhancements took place below geosynchronous orbit, emphasizing the need for more Medium Earth Orbit (MEO) missions.
PTEN is recognized as a prognostic-related biomarker and inhibits proliferation and invasiveness of skull base chordoma cells
Authors: Tian Kaibing , Ma Junpeng , Wang Ke , Li Da , Zhang Junting , Wang Liang , Wu Zhen
Published: September 2022

Objective

This work aimed to examine the function of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) in skull base chordoma (SBC) at the clinical and cellular levels.

Methods

Totally 65 paraffin-embedded and 86 frozen specimens from 96 patients administered surgery were analyzed. Immunohistochemical staining and quantitative real-time polymerase chain reaction were performed, and the associations of PTEN expression with clinical features were assessed. At the cellular level, PTEN was knocked down by the siRNA approach in the UCH-1 cell line, and cell proliferation and invasion were detected by the CCK-8 and migration assays, respectively.

Results

At the protein level, PTEN expression was increased in non-bone-invasive tumor samples in comparison with bone-invasive specimens (p = 0.025), and elevated in soft SBCs in comparison with hard tumors (p = 0.017). Increased PTEN protein expression was associated with decreased risk of tumor progression (p = 0.002; hazard ratio = 0.981, 95% confidence interval: 0.969–0.993). At the gene expression level, the cut-off value was set at 10.5 after ROC curve analysis, and SBC specimens were divided into two groups: PTEN high group, ΔCt value below 10.5; PTEN low group, ΔCt value above 10.5. In multivariate regression analysis of PFS, the risk of tumor progression was increased in PTEN low group tumors in comparison with PTEN high group SBCs (p = 0.006). In the CCK-8 assay, in comparison with control cells, PTEN knockdown cells had increased absorbance, suggesting elevated cell proliferation rate. In the invasion assay, the number of tumor cells penetrating into the lower chamber was significantly increased in the PTEN knockdown group compared with control cells.

Conclusions

Decreased PTEN expression in SBC, at the protein and gene levels, is associated with reduced PFS. PTEN knockdown in chordoma cells led to enhanced proliferation and invasiveness.
Case report: Balloon compression for cervical chyle leakage post neck dissection
Authors: Ding Zhaoming , Chen Mengshi , Pang Rui , Sheng Ruinan , Zhao Xuesong , Nie Chunlei
Published: September 2022

Postoperative chyle leakage (CL) is a rare but severe complication after neck dissection, and most patients with this complication can be treated conservatively. However, in patients with high-flow leakage, efficient and well-tolerated conservative treatment options are still lacking, and the treatments can be complicated. In this study, we report a case with CL of 1100 ml/day after neck dissection that was successfully treated by balloon compression.
Quantitative study of the effects of early standardized ambulation on sleep quality in patients after hepatectomy
Authors: Ni Chun-yan , Hou Guo-jun , Tang Ya-yuan , Wang Jing-jing , Chen Wen-jun , Yang Yuan , Wang Zhi-hong , Zhou Wei-ping
Published: September 2022

Background

Sleep quality has been always an important problem for patients after hepatectomy. The main purpose of the study is to investigate the effects of early ambulation on sleep quality in patients after liver resection via a quantitative study.

Methods

Patients undergoing liver tumor resection were randomly divided into two groups, and the Pittsburgh Sleep Quality Index (PSQI) was used to assess the postoperative activities and sleep quality.

Results

Patients who started early ambulation after liver resection had significantly better sleep quality, faster recovery of gastrointestinal function and shorter lengths of postoperative hospital stay compared with the control group. And there was no significant difference in the incidence of postoperative complications between the two groups.

Conclusion

Early standardized physical activities are feasible for patients after liver resection, which can significantly improve patient's sleep quality, reduce patient's pain and the nursing workload, and achieve rapid recovery.
Dynamic prediction model of fetal growth restriction based on support vector machine and logistic regression algorithm
Authors: Lian Cuiting , Wang Yan , Bao Xinyu , Yang Lin , Liu Guoli , Hao Dongmei , Zhang Song , Yang Yimin , Li Xuwen , Meng Yu , Zhang Xinyu , Li Ziwei
Published: September 2022

Background

This study analyzed the influencing factors of fetal growth restriction (FGR), and selected epidemiological and fetal parameters as risk factors for FGR.

Objective

To establish a dynamic prediction model of FGR.

Methods

This study used two methods, support vector machine (SVM) and multivariate logistic regression, to establish the prediction model of FGR at different gestational weeks.

Results

At 20–24 weeks and 25–29 weeks of gestation, the effect of the multivariate Logistic method on model prediction was better. At 30–34 weeks of gestation, the prediction effect of FGR model using the SVM method is better. The ROC curve area was above 85%.

Conclusions

The dynamic prediction model of FGR based on SVM and logistic regression is helpful to improve the sensitivity of FGR in pregnant women during prenatal screening. The establishment of prediction models at different gestational ages can effectively predict whether the fetus has FGR, and significantly improve the clinical treatment effect.
Case report: Ectopic thyroid tissue found in a liver with hepatocellular carcinoma
Authors: Wang Zhanbo , Yuan Jing , Li Jie
Published: September 2022

Background

Concomitant intrahepatic ectopic thyroid is rare in patients with hepatocellular carcinoma. Thyroid follicular structures outside the hepatocellular carcinoma lesions are regarded as satellite nodules or intrahepatic metastases of hepatocellular carcinoma, which often leads to misdiagnosis and overtreatment of hepatocellular carcinoma patients.

Case presentation

We report the case of an 83-year-old man with moderately differentiated hepatocellular carcinoma (2.5 cm) whose liver contained ectopic thyroid tissue. An encapsulated, multinodular grayish-yellow mass and multiple satellite nodules were detected and removed by right hepatic lobectomy. Microscopically, the main tumor displayed a predominant trabecular, cord-like structure. Liver tissue 0.5 cm from the tumor had a benign-appearing follicular thyroid structure. The follicles contained colloid tissue and were lined with low cuboidal cells with scant cytoplasm; lymphatic tissue was also present in the area. The hepatocellular carcinoma cells were positive for hepatocyte antigen and glypican-3 and negative for cytokeratin 19. The follicular thyroid cells expressed thyroglobulin, PAX8, and thyroid transcription factor-1. A metastatic thyroid neoplasm was excluded clinically and by ultrasound and computed tomography. One month after surgery, all of the patient's serological markers were normal; no tumor recurrence or metastasis has been detected for 7 postoperative months.

Conclusions

The finding of ectopic thyroid tissue in the liver of a patient with hepatocellular carcinoma is very rare. The possibility of hepatocellular carcinoma forming satellite nodules and intrahepatic metastasis should be ruled out first and immunohistochemistry may be definitive in making the diagnosis. Further examination is needed to exclude thyroid cancer liver metastases.
Clinical observation and risk assessment after splenectomy in hepatolenticular degeneration patients associated with hypersplenism
Authors: Zhang Wanzong , Yu Qingsheng , Peng Hui , Zheng Zhou , Zhou Fuhai
Published: September 2022

Background

Both hepatolenticular degeneration (HLD) and viral hepatitis B (HBV) can cause hypersplenism, but whether splenectomy is needed or can be performed in HLD patients associated with hypersplenism is still controversial. At present, HLD combined with hypersplenism has not been listed as the indication of splenectomy.

Objective

This study aimed to investigate the efficacy, risks, and postoperative complications of splenectomy in HLD patients associated with hypersplenism.

Methods

We retrospectively analyzed the clinical data of 180 HLD patients with hypersplenism who underwent splenectomy in the Department of General Surgery, First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, from January 2001 to December 2015. To evaluate the efficacy of splenectomy, the hemogram of white blood cells (WBC), red blood cells (RBC), platelets (PLT), and the liver function indexes including alanine aminotransferase, aspartate aminotransferase, and total bilirubin were recorded before surgery and 1, 3, 5, 7, and 14 days after surgery. In addition, the clinical data of 142 HBV patients with hypersplenism who underwent splenectomy over the same period were also recorded and compared with that of HLD patients. In particular, aiming to assess the risks of splenectomy in HLD, we also compared postoperative complications and 36-month mortality between the two groups.

Result

The level of WBC, RBC, and PLT were all elevated after splenectomy in both the HLD group and the HBV group. However, there was no significant difference in the variation of hemogram after splenectomy between the two groups (P > 0.05). Similarly, the variation of liver function indexes showed no statistical difference between the two groups. In terms of the incidence of postoperative complications including abdominal bleeding, pancreatic leakage, portal vein thrombosis treatment, incision infection, lung infection, and 36-month mortality, there were no significant differences between the two groups.

Conclusion

After splenectomy, the hemogram as well as liver function in the HLD group improved a lot and showed a consistent tendency with that in the HBV group. Meanwhile, compared to the HBV group, there was no significant difference in the incidence of postoperative complications in the HLD group. All these results indicate that splenectomy in HLD patients combined with hypersplenism is completely feasible and effective.
Early functional and therapeutic effect of reversed tumour shoulder prosthesis reconstruction after proximal humerus tumour resection
Authors: Wang Shang , Luo Yi , Wang Yitian , Zhang Yuqi , Gong Taojun , Tu Chongqi , Zhou Yong
Published: September 2022

Introduction

Reconstruction of proximal humeral tumours after resection is still controversial. And there are few articles describing oncology patients' postoperative function after reversed tumour shoulder prosthesis reconstruction. We investigated the functional results of patients who underwent reversed tumour shoulder prosthesis, including those who did not preserve the deltoid ending point.

Patients and methods

We retrospectively evaluated 16 patients with proximal humerus tumours who had undergone reversed tumour shoulder prosthesis. All patients underwent type Malawer I proximal humeral resection surgery and standard reverse tumour shoulder arthroplasty with a modular reverse shoulder prosthesis. We sutured the severed end of the deltoid to the brachialis muscle using the artificial patch for patients who had their deltoid ending point resected. Patients are rehabilitated and followed up according to our instructions.

Result

All patients were followed up for a mean of 27.4 months (13–59), and their mean age was 45.9 years (15–74). The mean length of the humeral resection was 11.6 cm (5–15). The mean shoulder mobility was 122° (82°–180°) in forward flexion; 39° (31°–45°) in posterior extension; 102° (65°–172°) in abduction; 43° (30°–60°) in external rotation; 83° (61°–90°) in internal rotation, and a mean MSTS score of 77.9% (63.3%–93.3%). The mean DASH score was 20.8 (2.5–35.8). The mean VAS score was 0.9. For patients who had their deltoid ending point resected, the mean length of the humeral resection was 14.0 cm; the mean shoulder mobility was 109° in forward flexion; 37.8° in posterior extension; 102.0° in abduction; 38.3° in external rotation; 86.3° in internal rotation, and the mean MSTS score was 78.8%; the mean DASH score was 21.6; the mean VAS score was 1.0.

Conclusion

Patients who underwent reverse tumour shoulder arthroplasty can achieve good early postoperative function, survival rate and low complication rate. In addition, patients who had their deltoid ending point removed also obtained good function after particular reconstruction.
Surgical technique of temporal muscle resuspension during cranioplasty for minimizing temporal hollowing: A case series
Authors: Yang Jingguo , Yang Xiaoyu , Wang Junjie , Yu Hang , You Chao , Ma Lu , Guan Junwen
Published: September 2022

Background

Temporal hollowing is a common but often overlooked complication following cranioplasty. To minimize temporal hollowing caused by temporal muscle contraction, we present the novel technique for temporal muscle resuspension during cranioplasty.

Methods

This is a retrospective case series which were done by a single surgeon at our university tertiary-A hospital between January 2019 and February 2020. The surgical technique was performed according to the forms of temporal muscle based on preoperative 3-D reconstruction and intraoperative images. All patients were followed up and evaluated on esthetic and functional outcomes.

Results

17 patients with an average age of 39.35 years, frontotemporoparietal cranial defect size of 78.85 cm2, and median follow-up of 7 months were included. The main cause of decompressive craniectomy was trauma (n = 15). Techniques of temporal muscle augmentative resuspension were performed. The follow-up esthetic and functional outcome evaluation showed that all patients had good postoperative results. No revision surgery was found among the patients.

Conclusions

This study proposes methods of temporal muscle augmentative resuspension based on forms of the muscle. We believe this might be of use in minimizing temporal hollowing after cranioplasty.
Bovine tuberculosis in youngstock cattle: A narrative review
Authors: Byrne Andrew W. , Barrett Damien , Breslin Philip , Fanning June , Casey Miriam , Madden Jamie M. , Lesellier Sandrine , Gormley Eamonn
Published: September 2022

Bovine tuberculosis (bTB), caused byMycobacterium bovis, remains a high-priority global pathogen of concern. The role of youngstock animals in the epidemiology of bTB has not been a focus of contemporary research. Here we have aimed to collate and summarize what is known about the susceptibility, diagnosis, transmission (infectiousness), and epidemiology toM. bovisin youngstock (up to 1-year of age). Youngstock are susceptible toM. bovisinfection when exposed, with the capacity to develop typical bTB lesions. Calves can be exposed through similar routes as adults,viaresidual infection, contiguous neighborhood spread, wildlife spillback infection, and the buying-in of infected but undetected cattle. Dairy systems may lead to greater exposure risk to calves relative to other production systems, for example,viapooled milk. Given their young age, calves tend to have shorter bTB at-risk exposure periods than older cohorts. The detection of bTB varies with age when using a wide range of ante-mortem diagnostics, also with post-mortem examination and confirmation (histological and bacteriological) of infection. When recorded as positive by ante-mortem test, youngstock appear to have the highest probabilities of any age cohort for confirmation of infection post-mortem. They also appear to have the lowest false negative bTB detection risk. In some countries, many calves are moved to other herds for rearing, potentially increasing inter-herd transmission risk. Mathematical models suggest that calves may also experience lower force of infection (the rate that susceptible animals become infected). There are few modeling studies investigating the role of calves in the spread and maintenance of infection across herd networks. One study found that calves, without operating testing and control measures, can help to maintain infection and lengthen the time to outbreak eradication. Policies to reduce testing for youngstock could lead to infected calves remaining undetected and increasing onwards transmission. Further studies are required to assess the risk associated with changes to testing policy for youngstock in terms of the impact for within-herd disease control, and how this may affect the transmission and persistence of infection across a network of linked herds.
Dietary supplementation with a mixture of herbal extracts during late gestation and lactation improves performance of sows and nursing piglets through regulation of maternal metabolism and transmission of antibodies
Authors: Wang Li , Huo Bin , Huang Lingjie , Che Lianqiang , Feng Bin , Lin Yan , Xu Shengyu , Wu De , Fang Zhengfeng
Published: September 2022

The dietary inclusion of phytogenic feed additives to improve the performance and health of sows is considered to be safe, effective and environmentally friendly, thus gaining growing popularity among new strategies. This study was designed with three trials aimed to determine the effective supplemental levels of Scutellaria baicalensis and Lonicera japonica mixed extracts (SLE) in sow diets based on production performance and explore its related mechanisms of action based on serum metabolites, antioxidant capacity, and immune profile of sows and nursing piglets. Trials 1 and 2 were conducted to determine the effective dose and ratio of SLE by supplementation of various proportions and doses of SLE to sows diets from the late pregnancy to weaning, with litter performance at farrowing and weaning and disease conditions being evaluated. Trial 3 was conducted to further explore the mechanisms of action of SLE as evaluated by serum immunity and antioxidants indices in late gestation and lactation sows. The results of trials 1 and 2 showed that dietary supplementation of 1.0 g/kg SLE (50% S. baicalensis extract, 30% L. japonica extract, and 20% wheat bran fiber as carrier) enhanced the number of piglets born alive, litter birth weight, litter weight gain, and average daily feed intake of sows during lactation, while decreased diarrhea of suckling piglets. In Trial 3, compared with the control group, dietary SLE supplementation increased (P < 0.05) sow serum glucose (GLU), triglyceride (TG), total cholesterol (TC), prolactin (PRL) and interleukin-10 (IL-10) concentrations, and total superoxide dismutase (T-SOD) activities at the farrowing, and increased (P < 0.05) sow serum prolactin, leptin, and insulin concentrations at d 14 of lactation. Fat concentrations in sow colostrum and in milk on day 14 of lactation, both IgA and IgG concentrations in colostrum, and both IL-10 and IgA concentrations in piglet serum at d 14 of lactation were all increased (P < 0.05) following dietary SLE supplementation. Altogether, dietary supplementation with the appropriate levels of SLE promoted health and growth of suckling piglets, which was associated with the improvement of maternal metabolism and transmission of antibodies.
The Impact of Paulownia Leaves Extract on Performance, Blood Biochemical, Antioxidant, Immunological Indices, and Related Gene Expression of Broilers
Authors: Sakr Shimaa A. , EL-Emam Huda A. , Naiel Mohammed A. E. , Wahed Noha M. , Zaher Hanan A. , Abougabal Mohammed Sh. , Alghamdi Youssef S. , Albogami Sarah , Soliman Mohamed Mohamed , Shukry Mustafa , Elghareeb Mona M.
Published: September 2022

The current research sought to assess the effects of paulownia leaves extract (PLE) on performance, blood hematological, antioxidant activity, and immunological response of broiler chicken. In total, two hundred 1-day-old male Cobb500 chicks were allocated randomly into four equal treatments with 5 replicates. The first treatment served as a control (CNT) and was fed the basal diet only, while the other treated treatments were fed on the basal diet supplemented with 0.1, 0.3, and 0.5 g/kg diet of PLE, respectively. The performance results showed significant increments (P < 0.05) in live body weight (LBW), weight gain (WG), and European production efficiency factors (EPEIs) (linearly; p < 0.001) in cooperated with increasing PLE levels in broiler diets. At the same time, feed conversion ratio (FCR) and livability percentages were numerically enhanced under the effects of PLE supplementation. Moreover, a notable increase (P < 0.05 or 0.01) in oxidative remarks activity (GSH, glutathione; SOD, super oxide-dismutase and CAT, catalase) and elevated levels of immunoglobulin (IgM, immunoglobulin M and IgG, immunoglobulin G) were noted (P < 0.05) for treatments fed with PLE in a dose-dependent manner. Also, a dramatic linear increase was observed in mRNA expression of IGF-1, GHR, IL-1β, and IL-10 genes of broiler chickens. This study concluded that enriched broiler feeds with 0.5 g/kg PLE might be a beneficial strategy to promote broiler health and production.
Isolation and characterization of avian leukosis virus subgroup J associated with hemangioma and myelocytoma in layer chickens in China
Authors: Wu Lan , Li Youjun , Chen Xueyang , Yang Yuxin , Fang Chun , Gu Yufang , Liu Jing , Liang Xiongyan , Yang Yuying
Published: September 2022

A strain of avian leukosis virus (ALV) belonging to a new envelope subgroup J (ALV-J) emerged in 1988 as a new subgroup of ALV and spread rapidly throughout the world. Due to the infection and spread of ALV-J, the global poultry industry experienced a significant loss. Although the disease had been prevented and controlled effectively by culling domestic chickens in the infected zone, a few field cases of ALV-J infection were reported in China in recent years. This study was conducted to characterize the genome and analyze the lesions and histopathology of the ALV-J strain named HB2020, which was isolated from layer chickens in Hubei Province, China. The full-length proviral genome sequence analysis of ALV-J HB2020 revealed that it was a recombinant strain of ev-1 and HPRS-103 in the gag gene in comparison to ALV-J prototype HPRS-103. In the 3′-untranslated region (3'UTR) of the nucleotide sequence, there were found 205-base pairs (bp) deletion, of which 175 were detected in the redundant transmembrane (rTM) region. Besides, the surface glycoprotein gene gp85 had five mutations in a conservative site, whereas the transmembrane protein gene gp37 was relatively conserved. The animal experiments conducted later on this strain have shown that HB2020 can cause various neoplastic lesions in chickens, including enlarged livers with hemangiomas and spleens with white nodules. Additionally, as the exposure time increased, the number of tumor cells that resembled myelocytes in the blood smears of infected chickens gradually increased. These results indicated that HB2020 on recombination with ALV subgroup E (ALV-E) and ALV-J could induce severe hemangiomas and myelocytomas. This inference might provide a molecular basis for further research about the pathogenicity of ALV and emphasize the need for control and prevention of avian leukosis.
Benchmarking calf health: Assessment tools for dairy herd health consultancy based on reference values from 730 German dairies with respect to seasonal, farm type, and herd size effects
Authors: Dachrodt Linda , Bartel Alexander , Arndt Heidi , Kellermann Laura Maria , Stock Annegret , Volkmann Maria , Boeker Andreas Robert , Birnstiel Katrin , Do Duc Phuong , Klawitter Marcus , Paul Philip , Stoll Alexander , Woudstra Svenja , Knubben-Schweizer Gabriela , Müller Kerstin Elisabeth , Hoedemaker Martina
Published: September 2022

Good calf health is crucial for a successfully operating farm business and animal welfare on dairy farms. To evaluate calf health on farms and to identify potential problem areas, benchmarking tools can be used by farmers, herd managers, veterinarians, and other advisory persons in the field. However, for calves, benchmarking tools are not yet widely established in practice. This study provides hands-on application for on-farm benchmarking of calf health. Reference values were generated from a large dataset of the “PraeRi” study, including 730 dairy farms with a total of 13,658 examined preweaned dairy calves. At herd level, omphalitis (O, median 15.9%) was the most common disorder, followed by diarrhea (D, 15.4%) and respiratory disease (RD, 2.9%). Abnormal weight bearing (AWB) was rarely detected (median, 0.0%). Calves with symptoms of more than one disorder at the same time (multimorbidity, M) were observed with a prevalence of 2.3%. The enrolled farms varied in herd size, farm operating systems, and management practices and thus represented a wide diversity in dairy farming, enabling a comparison with similar managed farms in Germany and beyond. To ensure comparability of the data in practice, the reference values were calculated for the whole data set, clustered according to farm size (1–40 dairy cows (n= 130), 41–60 dairy cows (n= 99), 61–120 dairy cows (n= 180), 121–240 dairy cows (n= 119) and farms with more than 240 dairy cows (n= 138), farm operating systems (conventional (n= 666), organic (n= 64)) and month of the year of the farm visit. There was a slight tendency for smaller farms to have a lower prevalence of disorders. A statistically significant herd-size effect was detected for RD (p= 0.008) and D (p< 0.001). For practical application of these reference values, tables, diagrams, and an Excel®(Microsoft®) based calf health calculator were developed as tools for on-farm benchmarking (https://doi.org/10.6084/m9.figshare.c.6172753). In addition, this study provides a detailed description of the colostrum, feeding and housing management of preweaned calves in German dairy farms of different herd sizes and farm type (e.g., conventional and organic).
Effects of Pgam1-mediated glycolysis pathway in Sertoli cells on Spermatogonial stem cells based on transcriptomics and energy metabolomics
Authors: An Xuejiao , Li Qiao , Chen Nana , Li Taotao , Wang Huihui , Su Manchun , Shi Huibin , Ma Youji
Published: September 2022

Spermatogenesis is a complex process involving a variety of intercellular interactions and precise regulation of gene expression. Spermatogenesis is sustained by a foundational Spermatogonial stem cells (SSCs) and in mammalian testis. Sertoli cells (SCs) are the major component of SSC niche. Sertoli cells provide structural support and supply energy substrate for developing germ cells. Phosphoglycerate mutase 1 (Pgam1) is a key enzyme in the glycolytic metabolism and our previous work showed that Pgam1 is expressed in SCs. In the present study, hypothesized that Pgam1-depedent glycolysis in SCs plays a functional role in regulating SSCs fate decisions. A co-culture system of murine SCs and primary spermatogonia was constructed to investigate the effects of Pgam1 knockdown or overexpression on SSCs proliferation and differentiation. Transcriptome results indicated that overexpression and knockdown of Pgam1 in SCs resulted in up-regulation of 458 genes (117 down-regulated, 341 up-regulated) and down-regulation of 409 genes (110 down-regulated, 299 up-regulated), respectively. Further analysis of these DEGs revealed that GDNF, FGF2 and other genes that serve key roles in SSCs niche maintenance were regulated by Pgam1. The metabolome results showed that a total of 11 and 16 differential metabolites were identified in the Pgam1 gene overexpression and knockdown respectively. Further screening of these metabolites indicated that Sertoli cell derived glutamate, glutamine, threonine, leucine, alanine, lysine, serine, succinate, fumarate, phosphoenolpyruvate, ATP, ADP, and AMP have potential roles in regulating SSCs proliferation and differentiation. In summary, this study established a SCs-SSCs co-culture system and identified a list of genes and small metabolic molecules that affect the proliferation and differentiation of SSCs. This study provides additional insights into the regulatory mechanisms underlying interactions between SCs and SSCs during mammalian spermatogenesis.
Impact of progesterone on innate immunity and cell death after influenza A virus H1N1 2009 infection of lung and placental cells in vitro
Authors: Li Miranda , Li Amanda , Huang Hazel , Munson Jeff , Obadan Adebimpe , Fuller Deborah H. , Adams Waldorf Kristina M.
Published: September 2022

The influenza A virus (IAV) 2009 H1N1 pandemic was associated with an increased risk of maternal mortality, preterm birth, and stillbirth. The underlying mechanism for severe maternal lung disease and stillbirth is incompletely understood, but IAV infection is known to activate innate immunity triggering the release of cytokines. Elucidating the impact of progesterone (P4), a key hormone elevated in pregnancy, on the innate immune and inflammatory response to IAV infection is a critical step in understanding the pathogenesis of adverse maternal-fetal outcomes. IAV H1N1 pdm/09 was used to infect cell lines Calu-3 (lung adenoma) and ACH-3P (extravillous trophoblast) with or without P4 (100 nM) at multiplicity of infections (MOI) 0, 0.5, and 3. Cells were harvested at 24 and 48 hours post infection (hpi) and analyzed for cytopathic effects (CPE), replicating virus (TCID50), cytotoxicity (Lactate Dehydrogenase (LDH) assay), and NLRP3 inflammasome activation (caspase-1 activity, fluorometric assay). Activation of antiviral innate immunity was quantified (RT-qPCR, Luminex) by measuring biomarker gene and protein expression of innate immune activation (IFIT1, IFNB), inflammation (IL6), interferon signaling (MXA), chemokines (IL-8, IL-10). Both Calu-3 and ACH-3P were highly permissible to IAV infection at each timepoint as demonstrated by CPE and recovery of replicating virus. In Calu-3, progesterone treatment was associated with a significant increase in cytotoxicity, increased gene expression of IL6, and increased protein expression of IFN-β, IL-6, and IL-18. Conversely, in ACH-3P, progesterone treatment was associated with significantly suppressed cytotoxicity, decreased gene expression of IFNB, IL6 and IL1B, and increased protein expression of IFN-β and IL-6. In both cell lines, caspase-1 activity was significantly decreased after progesterone treatment, indicating NLRP3 inflammasome activation was not underlying the higher cell death in Calu-3. In summary, these data provide evidence that progesterone plays a dual role by ameliorating viral infection in the placenta but exacerbating influenza A virus-associated injury in the lung through nongenomic modulation of the innate immune response.