bluuebunny/crossref_metadata_2025 huggingface dataset:
Ectopic overexpression of a type-II DGAT (CeDGAT2-2) derived from oil-rich tuber of Cyperus esculentus enhances accumulation of oil and oleic acid in tobacco leaves
Authors: Gao Yu , Sun Yan , Gao Huiling , Chen Ying , Wang Xiaoqing , Xue Jinai , Jia Xiaoyun , Li Runzhi
Published: March 2021

Background

Engineering triacylglycerol (TAG) accumulation in vegetative tissues of non-food crops has become a promising way to meet our increasing demand for plant oils, especially the renewable production of biofuels. The most important target modified in this regard is diacylglycerol acyltransferase (DGAT) enzyme responsible for the final rate-limiting step in TAG biosynthesis. Cyperus esculentus is a unique plant largely accumulating oleic acid-enriched oil in its underground tubers. We speculated that DGAT derived from such oil-rich tubers could function more efficiently than that from oleaginous seeds in enhancing oil storage in vegetative tissues of tobacco, a high-yielding biomass crops.

Results

Three CeDGAT genes namely CeDGAT1, CeDGAT2-1 and CeDGAT2-2 were identified in C. esculentus by mining transcriptome of developing tubers. These CeDGATs were expressed in tissues tested, with CeDGAT1 highly in roots, CeDGAT2-1 abundantly in leaves, and CeDGAT2-2 predominantly in tubers. Notably, CeDGAT2-2 expression pattern was in accordance with oil dynamic accumulation during tuber development. Overexpression of CeDGAT2-2 functionally restored TAG biosynthesis in TAG-deficient yeast mutant H1246. Oleic acid level was significantly increased in CeDGAT2-2 transgenic yeast compared to the wild-type yeast and ScDGA1-expressed control under culture with and without feeding of exogenous fatty acids. Overexpressing CeDGAT2-2 in tobacco led to dramatic enhancements of leafy oil by 7.15- and 1.7-fold more compared to the wild-type control and plants expressing Arabidopsis seed-derived AtDGAT1. A substantial change in fatty acid composition was detected in leaves, with increase of oleic acid from 5.1% in the wild type to 31.33% in CeDGAT2-2-expressed tobacco and accompanied reduction of saturated fatty acids. Moreover, the elevated accumulation of oleic acid-enriched TAG in transgenic tobacco exhibited no significantly negative impact on other agronomic traits such as photosynthesis, growth rates and seed germination except for small decline of starch content.

Conclusions

The present data indicate that CeDGAT2-2 has a high enzyme activity to catalyze formation of TAG and a strong specificity for oleic acid-containing substrates, providing new insights into understanding oil biosynthesis mechanism in plant vegetative tissues. Overexpression of CeDGAT2-2 alone can significantly increase oleic acid-enriched oil accumulation in tobacco leaves without negative impact on other agronomy traits, showing CeDGAT2-2 as the desirable target gene in metabolic engineering to enrich oil and value-added lipids in high-biomass plants for commercial production of biofuel oils.
The IS6 family, a clinically important group of insertion sequences including IS26
Authors: Varani Alessandro , He Susu , Siguier Patricia , Ross Karen , Chandler Michael
Published: March 2021

The IS6family of bacterial and archaeal insertion sequences, first identified in the early 1980s, has proved to be instrumental in the rearrangement and spread of multiple antibiotic resistance. Two IS, IS26(found in many enterobacterial clinical isolates as components of both chromosome and plasmids) and IS257(identified in the plasmids and chromosomes of gram-positive bacteria), have received particular attention for their clinical impact. Although few biochemical data are available concerning the transposition mechanism of these elements, genetic studies have provided some interesting observations suggesting that members of the family might transpose using an unexpected mechanism. In this review, we present an overview of the family, the distribution and phylogenetic relationships of its members, their impact on their host genomes and analyse available data concerning the particular transposition pathways they may use. We also provide a mechanistic model that explains the recent observations on one of the IS6family transposition pathways: targeted cointegrate formation between replicons.
Molecular survey of mcr1 and mcr2 plasmid mediated colistin resistance genes in Escherichia coli isolates of animal origin in Iran
Authors: Ilbeigi Kayhan , Askari Badouei Mahdi , Vaezi Hossein , Zaheri Hassan , Aghasharif Sina , Kafshdouzan Khatereh
Published: March 2021

Objectives

The emergence of colistin-resistant Enterobacteriaceae from human and animal sources is one of the major public health concerns as colistin is the last-resort antibiotic for treating infections caused by multidrug-resistant Gram-negative bacteria. We aimed to determine the prevalence of the prototype widespread colistin resistance genes (mcr-1 and mcr-2) among commensal and pathogenic Escherichia coli strains isolated from food-producing and companion animals in Iran.

Results

A total of 607 E. coli isolates which were previously collected from different animal sources between 2008 and 2016 used to uncover the possible presence of plasmid-mediated colistin resistance genes (mcr-1 and mcr-2) by PCR. Overall, our results could not confirm the presence of any mcr-1 or mcr-2 positive E. coli among the studied isolates. It is concluded that despite the important role of food-producing animals in transferring the antibiotic resistance, they were not the main source for carriage of mcr-1 and mcr-2 in Iran until 2016. This study suggests that the other mcr variants (mcr-3 to mcr-9) might be responsible for conferring colistin resistance in animal isolates in Iran. The possible linkage between pig farming industry and high level of mcr carriage in some countries needs to be clarified in future prospective studies.
Multi-omics integration identifies key upstream regulators of pathomechanisms in hypertrophic cardiomyopathy due to truncating MYBPC3 mutations
Authors: Pei J. , Schuldt M. , Nagyova E. , Gu Z. , el Bouhaddani S. , Yiangou L. , Jansen M. , Calis J. J. A. , Dorsch L. M. , Blok C. Snijders , van den Dungen N. A. M. , Lansu N. , Boukens B. J. , Efimov I. R. , Michels M. , Verhaar M. C. , de Weger R. , Vink A. , van Steenbeek F. G. , Baas A. F. , Davis R. P. , Uh H. W. , Kuster D. W. D. , Cheng C. , Mokry M. , van der Velden J. , Asselbergs F. W. , Harakalova M.
Published: March 2021

Background

Hypertrophic cardiomyopathy (HCM) is the most common genetic disease of the cardiac muscle, frequently caused by mutations in MYBPC3. However, little is known about the upstream pathways and key regulators causing the disease. Therefore, we employed a multi-omics approach to study the pathomechanisms underlying HCM comparing patient hearts harboring MYBPC3 mutations to control hearts.

Results

Using H3K27ac ChIP-seq and RNA-seq we obtained 9310 differentially acetylated regions and 2033 differentially expressed genes, respectively, between 13 HCM and 10 control hearts. We obtained 441 differentially expressed proteins between 11 HCM and 8 control hearts using proteomics. By integrating multi-omics datasets, we identified a set of DNA regions and genes that differentiate HCM from control hearts and 53 protein-coding genes as the major contributors. This comprehensive analysis consistently points toward altered extracellular matrix formation, muscle contraction, and metabolism. Therefore, we studied enriched transcription factor (TF) binding motifs and identified 9 motif-encoded TFs, including KLF15, ETV4, AR, CLOCK, ETS2, GATA5, MEIS1, RXRA, and ZFX. Selected candidates were examined in stem cell-derived cardiomyocytes with and without mutated MYBPC3. Furthermore, we observed an abundance of acetylation signals and transcripts derived from cardiomyocytes compared to non-myocyte populations.

Conclusions

By integrating histone acetylome, transcriptome, and proteome profiles, we identified major effector genes and protein networks that drive the pathological changes in HCM with mutated MYBPC3. Our work identifies 38 highly affected protein-coding genes as potential plasma HCM biomarkers and 9 TFs as potential upstream regulators of these pathomechanisms that may serve as possible therapeutic targets.
Mesenchymal stem cells cultured in serum-free medium ameliorate experimental peritoneal fibrosis
Authors: Nagasaki Kohei , Nakashima Ayumu , Tamura Ryo , Ishiuchi Naoki , Honda Kiyomasa , Ueno Toshinori , Doi Shigehiro , Kato Yukio , Masaki Takao
Published: March 2021

Background

Mesenchymal stem cells (MSCs) provide potential treatments for peritoneal fibrosis. However, MSCs cultured in media containing serum bring risks of infection and other problems. In this study, we compared the effect of human MSCs in serum-free medium (SF-MSCs) on peritoneal fibrosis with that of MSCs cultured in medium containing 10% fetal bovine serum (10%MSCs).

Methods

Peritoneal fibrosis was induced by intraperitoneally injecting 0.1% chlorhexidine gluconate (CG). SF-MSCs or 10%MSCs were intraperitoneally administered 30 min after the CG injection. Ten days after the CG and MSC injections, we performed histological analyses and peritoneal equilibrium testing. In the in vitro experiments, we used transforming growth factor (TGF)-β1-stimulated human peritoneal mesothelial cells incubated in conditioned medium from MSCs to examine whether the SF-MSCs showed enhanced ability to produce antifibrotic humoral factors.

Results

Histological staining showed that the SF-MSCs significantly suppressed CG-induced cell accumulation and thickening compared with that of the 10%MSCs. Additionally, the SF-MSCs significantly inhibited mesenchymal cell expression, extracellular matrix protein deposition and inflammatory cell infiltration. Peritoneal equilibration testing showed that compared with administering 10%MSCs, administering SF-MSCs significantly reduced the functional impairments of the peritoneal membrane. The in vitro experiments showed that although the conditioned medium from MSCs suppressed TGF-β1 signaling, the suppression did not significantly differ between the SF-MSCs and 10%MSCs.

Conclusions

Serum-free culture conditions can enhance the antifibrotic abilities of MSCs by suppressing inflammation. Administering ex vivo expanded SF-MSCs may be a potential therapy for preventing peritoneal fibrotic progression.
Why and how to engage expert stakeholders in ontology development: insights from social and behavioural sciences
Authors: Norris Emma , Hastings Janna , Marques Marta M. , Mutlu Ailbhe N. Finnerty , Zink Silje , Michie Susan
Published: March 2021

Background

Incorporating the feedback of expert stakeholders in ontology development is important to ensure content is appropriate, comprehensive, meets community needs and is interoperable with other ontologies and classification systems. However, domain experts are often not formally engaged in ontology development, and there is little available guidance on how this involvement should best be conducted and managed. Social and behavioural science studies often involve expert feedback in the development of tools and classification systems but have had little engagement with ontology development. This paper aims to (i) demonstrate how expert feedback can enhance ontology development, and (ii) provide practical recommendations on how to conduct expert feedback in ontology development using methodologies from the social and behavioural sciences.

Main body

Considerations for selecting methods for engaging stakeholders are presented. Mailing lists and issue trackers as existing methods used frequently in ontology development are discussed. Advisory boards and working groups, feedback tasks, consensus exercises, discussions and workshops are presented as potential methods from social and behavioural sciences to incorporate in ontology development.

Conclusions

A variety of methods from the social and behavioural sciences exist to enable feedback from expert stakeholders in ontology development. Engaging domain experts in ontology development enables depth and clarity in ontology development, whilst also establishing advocates for an ontology upon its completion.
Growth optimization and identification of an ω-transaminase by a novel native PAGE activity staining method in a Bacillus sp. strain BaH isolated from Iranian soil
Authors: Gord Noshahri Najme , Fooladi Jamshid , Engel Ulrike , Muller Delphine , Kugel Michaela , Gorenflo Pascal , Syldatk Christoph , Rudat Jens
Published: March 2021

ω-Transaminases’ (ω-TAs) importance for synthesizing chiral amines led to the development of different methods to quickly identify and characterize new sources of these enzymes. Here we describe the optimization of growth and induction of such an enzyme in a wild type strain of Bacillus sp. strain BaH (IBRC-M 11337) isolated from Iranian soil in shaking flasks by the response surface methodology (RSM). Optimum conditions were set in a multiplexed bench-top bioreactor system (Sixfors). ω-TA activity of obtained biomass was checked by an innovative efficient colorimetric assay for localizing ω-TAs in crude extracts on acrylamide gel by using ortho-xylylenediamine (OXD) as amino donor. The application of the established OXD assay is thereby expanded from high-throughput activity screenings and colony-based screenings of heterologously expressed mutants to a direct identification of ω-TAs in wild-type strains: This assay can be used to detect the protein band of the respective enzyme in crude extracts of novel isolates by visual inspection of native PAGEs without any upstream protein purification, thus enabling subsequent further investigations of a newly discovered enzyme directly from the crude extract.
Energy planning for an eco-city based on a distributed energy network
Authors: Zhu Xiaojun , Zhu Jianhua , Wu Bencheng , Liu Minsheng , Wang Guoqing , Zhu Zhenqi , Gan Zhongxue , Zhang Jing , Meng Chuizhou
Published: March 2021

Background

In China, traditional energy planning is subordinate to city planning, with a primary purpose of meeting the energy demand in urban areas by planning and designing an energy system. However, most of the current energy planning in China pertains to a low-efficient system and proves to be unfriendly to the environment, which is no longer appropriate for the future development of modern industrial parks and cities.

Methods

A combined cooling, heating, gas and power energy network with distributed cogeneration units and renewable energy sources provides an effective solution to the related problems. In this paper, a new energy planning method for the Eco-City is proposed, which is based on a Distributed Energy Network (DEN) system. The designed method of a four-stage energy integration mode is implemented, which includes prediction of the energy demand, optimization of the energy utilization, matching of the energy supply and the recovery side. Based on the planning and designing of the DEN, the technical and economic analysis draw reasonable results.

Results

As implemented in the practical case of the Sino-German Eco-Park, the procedure of the proposed energy planning method is comprehensively explained. Finally, by reviewing the planning, design and operation, the current state and problems of the Sino-German Eco-Park are described.

Conclusions

A new energy design for Eco-Parks is developed as a result of a thorough analysis of the planning process, which integrates city planning and energy planning together and provides energy solutions for high-level cities and industrial parks. The four-stage integrated energy model provides a guideline for the entire process and its detailed steps. Relevant experiences and lessons from the energy planning of the Sino-German Eco-Park are of significance for supporting similar projects in the future.
Integrating patient perspectives in the development of a mobile health intervention to address chronic pain and heavy drinking in primary care: a qualitative study of patients in an urban, safety-net hospital setting
Authors: Palfai Tibor P. , Kratzer Maya P. L. , Morone Natalia E. , Bernstein Judith A.
Published: March 2021

Background

Chronic pain and heavy drinking are conditions that commonly co-occur among primary care patients. Despite the availability of behavioral interventions that target these conditions individually, engagement and adherence to treatment remain a challenge, and there have been no interventions designed to address both of these conditions together for patients presenting to primary care. This study seeks to incorporate the perspectives of patients regarding symptoms, treatment experiences, views on behavior change, and technology use to develop a tailored, integrated mobile health intervention that addresses both pain and heavy drinking among patients in primary care.

Methods

Twelve participants with moderate or greater chronic pain intensity and heavy drinking were recruited from primary care clinics in a large urban safety-net hospital. One-on-one interviews were recorded and transcribed. Codes were developed from interview transcripts, followed by thematic analysis in which specific meanings were assigned to codes. Participants also completed a series of Likert-based rating scales to evaluate components of the proposed intervention to supplement qualitative interviews.

Results

A number of themes were identified that had implications for intervention tailoring including: ambivalence about changing drinking, low expectations about pain treatment success, desire for contact with a designated provider, common use of smartphones but lack of familiarity with functions as a potential barrier to use, and strategies to maintain engagement and adherence. Evaluative ratings indicated that the proposed intervention content was perceived as helpful and the proposed structure, layout and design of the mobile intervention was acceptable to patients.

Conclusions

Results supported the view that a mobile health intervention delivered via smartphone with electronic coaching is an acceptable method of addressing chronic pain and heavy drinking among patients in primary care. The interviews highlight the need to utilize an intervention approach that addresses motivation to change drinking, sets realistic expectations for change, provides careful attention to training/education of the use of technology components, and fosters engagement through the use of reminders, feedback, and personalized activities.
The influence of supraliminal priming on energy density of food selection: a randomised control trial
Authors: Schlegel Isabelle , Carstairs Sharon A. , Ozakinci Gozde
Published: March 2021

Background

Many people exercise because they know it is good for their health. Although this is true, it can make us feel deserving of a reward and lead us to eat more indulgent, less healthy food than if we had not done any exercise. Generally, lower energy-dense (LED) foods are recognised as healthier choices than higher energy-dense (HED) options. Despite our intention to make healthy choices, seeing tempting higher-calorie foods on offer often side-tracks us. Priming is a psychological tool that makes specific changes to our environment that remind us of our motivation to be healthy. This makes it easier to choose a healthier option, by nudging us towards it without us even realising. However, it is currently unclear which method of priming achieves the best results.

Aims

Our study explores whether priming people to expect they will receive LED food leads them to make this healthier choice after exercise, even when also offered tempting less healthy HED foods at the moment of selection.

Methods

Our study observed the foods selected by university athletes after their sports matches. Before the match, half of the participants were primed by asking them to choose a LED snack from the options we offered, which they would receive after the match. The remaining half of participants were not asked this same question. To distract the athletes from our observation of their food choices, participants completed a task prior to choosing their snack, which was disguised as a ‘thank you’ for taking part.

Results

Overall, we found the priming group did not choose LED foods significantly more than the control group, hence priming did not increase LED food selection.

Conclusion

Importantly, our results indicate that priming must be more noticeable to achieve its goal. Additionally, we demonstrated that priming may be less successful for young athletic individuals, compared to older and more overweight adults recruited in other studies. This highlights the importance of studying a broader demographic range of individuals from the general population. We support future research into this area, which will help us to tweak priming to achieve the best outcomes.

Trial registration

ISRCTN Registry, ISRCTN74601698. Date registered: 02/10/2020 (retrospectively registered).
Going underground: short- and long-term movements may reveal the fossorial spatial ecology of an amphisbaenian
Authors: Martín José , Ortega Jesús , García-Roa Roberto , Jiménez-Robles Octavio , Rodríguez-Ruiz Gonzalo , Recio Pablo , Cuervo José Javier
Published: March 2021

Background

The movement and spatial ecology of an animal depends on its morphological and functional adaptations to its environment. In fossorial animals, adaptations to the underground life help to face peculiar ecological challenges, very different from those of epigeal species, but may constrain their movement ability.

Methods

We made a long-term capture-recapture study of the strictly fossorial amphisbaenian reptileTrogonophis wiegmannito analyze its long-term movement patterns. We also used passive integrated transponder (PIT) telemetry to detect and follow undisturbed individuals underground, obtaining data of their short-term movement patterns.

Results

Amphisbaenians showed a high site fidelity, moving short distances and over small areas, and spending some days without any noticeable movement, even under favorable conditions. We also found differences in movements between sexes and age classes.

Conclusions

This movement and spatial strategy can be related to the energetic constrains of underground burrowing, or to the low metabolic requirements of fossorial reptiles, as distances and areas covered were much smaller than for epigeal reptiles of similar size. Individual differences probably reflect differential reproductive and social requirements of males and females, and that younger individuals might show more floating behavior until they can settle in a territory. This study is a rare example describing the movement ecology of a fossorial species and may contribute to the general understanding of the factors that affect space use and movement decisions in animals.
Space-occupying brain lesions, trauma-related tau astrogliopathy, and ARTAG: a report of two cases and a literature review
Authors: Bachstetter Adam D. , Garrett Filip G. , Jicha Gregory A. , Nelson Peter T.
Published: March 2021

Astrocytes with intracellular accumulations of misfolded phosphorylated tau protein have been observed in advanced-stage chronic traumatic encephalopathy (CTE) and in other neurodegenerative conditions. There is a growing awareness that astrocytic tau inclusions are also relatively common in the brains of persons over 70 years of age—affecting approximately one-third of autopsied individuals. The pathologic hallmarks of aging-related tau astrogliopathy (ARTAG) include phosphorylated tau protein within thorn-shaped astrocytes (TSA) in subpial, subependymal, perivascular, and white matter regions, whereas granular-fuzzy astrocytes are often seen in gray matter. CTE and ARTAG share molecular and histopathologic characteristics, suggesting that trauma-related mechanism(s) may predispose to the development of tau astrogliopathy. There are presently few experimental systems to study the pathobiology of astrocytic-tau aggregation, but human studies have made recent progress. For example, leucotomy (also referred to as lobotomy) is associated with a localized ARTAG-like neuropathology decades after the surgical brain injury, suggesting that chronic brain injury of any type may predispose to later life ARTAG. To examine this idea in a different context, we report clinical and pathologic features of two middle-aged men who came to autopsy with large (> 6 cm in greatest dimension) arachnoid cysts that had physically displaced and injured the subjects’ left temporal lobes through chronic mechanical stress. Despite the similarity of the size and location of the arachnoid cysts, these individuals had dissimilar neurologic outcomes and neuropathologic findings. We review the evidence for ARTAG in response to brain injury, and discuss how the location and molecular properties of astroglial tau inclusions might alter the physiology of resident astrocytes. These cases and literature review point toward possible mechanism(s) of tau aggregation in astrocytes in response to chronic brain trauma.
Coping with brain amyloid: genetic heterogeneity and cognitive resilience to Alzheimer’s pathophysiology
Authors:   for the Alzheimer’s Disease Neuroimaging Initiative (ADNI), Ramanan Vijay K. , Lesnick Timothy G. , Przybelski Scott A. , Heckman Michael G. , Knopman David S. , Graff-Radford Jonathan , Lowe Val J. , Machulda Mary M. , Mielke Michelle M. , Jack Clifford R. , Petersen Ronald C. , Ross Owen A. , Vemuri Prashanthi
Published: March 2021

Although abnormal accumulation of amyloid in the brain is an early biomarker of Alzheimer’s disease (AD), wide variation in cognitive trajectories during life can be seen in the setting of brain amyloidosis, ranging from maintenance of normal function to progression to dementia. It is widely presumed that cognitive resilience (i.e., coping) to amyloidosis may be influenced by environmental, lifestyle, and inherited factors, but relatively little in specifics is known about this architecture. Here, we leveraged multimodal longitudinal data from a large, population-based sample of older adults to discover genetic factors associated with differential cognitive resilience to brain amyloidosis determined by positron emission tomography (PET). Among amyloid-PET positive older adults, the AD risk allele APOE ɛ4 was associated with worse longitudinal memory trajectories as expected, and was thus covaried in the main analyses. Through a genome-wide association study (GWAS), we uncovered a novel association with cognitive resilience on chromosome 8 at the MTMR7/CNOT7/ZDHHC2/VPS37A locus (p = 4.66 × 10–8, β = 0.23), and demonstrated replication in an independent cohort. Post-hoc analyses confirmed this association as specific to the setting of elevated amyloid burden and not explained by differences in tau deposition or cerebrovascular disease. Complementary gene-based analyses and publically available functional data suggested that the causative variant at this locus may tag CNOT7 (CCR4-NOT Transcription Complex Subunit 7), a gene linked to synaptic plasticity and hippocampal-dependent learning and memory. Pathways related to cell adhesion and immune system activation displayed enrichment of association in the GWAS. Our findings, resulting from a unique study design, support the hypothesis that genetic heterogeneity is one of the factors that explains differential cognitive resilience to brain amyloidosis. Further characterization of the underlying biological mechanisms influencing cognitive resilience may facilitate improved prognostic counseling, therapeutic application, and trial enrollment in AD.
Separation of magnetotelluric signals based on refined composite multiscale dispersion entropy and orthogonal matching pursuit
Authors: Zhang Xian , Li Jin , Li Diquan , Li Yong , Liu Bei , Hu Yanfang
Published: March 2021

Magnetotelluric (MT) data processing can increase the reliability of measured data. Traditional MT data denoising methods are usually applied to entire MT time-series, which results in the loss of useful MT signals and a decrease of imaging accuracy of electromagnetic inversion. However, targeted MT noise separation can retain part of the signal unaffected by strong noise and enhance the quality of MT responses. Thus, we propose a novel method for MT noise separation that uses the refined composite multiscale dispersion entropy (RCMDE) and the orthogonal matching pursuit (OMP) algorithm. First, the RCMDE is extracted from each segment of the MT data. Then, the RCMDEs for each segment are input to the fuzzy c-mean (FCM) clustering algorithm for automatic identification of the MT signal and noise. Next, the OMP method is utilized to remove the identified noise segments independently. Finally, the reconstructed signal consists of the denoised signal segments and the identified useful signal segments. We conducted simulation experiments and algorithm evaluations on electromagnetic transfer function (EMTF) data, simulated data and measured sites. The results indicate that the RCMDE can improve the stability of multiscale dispersion entropy (MDE) and multiscale entropy (ME) by analyzing the characteristics of the signal samples library, effectively distinguishing MT signals and noise. Compared with the existing technique of denoising entire time series, the proposed method uses the RCMDE as characteristic parameter and uses the OMP algorithm for noise separation, simplifies the multi-feature fusion, and improves the accuracy of signal-noise identification. Moreover, the denoising efficiency is accelerated, and the MT response in the low-frequency band is greatly improved.
Production of oligomeric procyanidins by mild steam explosion treatment of grape seeds
Authors: Zhang Jie , Liu Dan , Wang Aoke , Cheng Li , Wang Wenya , Liu Yanhui , Ullah Sadeeq , Yuan Qipeng
Published: March 2021

Background

Sixty five percent of procyanidins in grape seeds is polymeric procyanidins (PPC), and they could not be assimilated directly by human. To enhance procyanidin assimilation, steam explosion treatment (SE) was used to facilitate the preparation of oligomeric procyanidins (OPC) from grape seeds.

Results

The results indicate that SE treatment made grape seeds loose and porous, and decreased the mean degree of polymerization (mDP) of procyanidins. The procyanidins content and total phenolic content (TPC) were decreased with the increase of SE severity, while the amount of catechin (CA), epicatechin (EC) and epicatechin-3-O-gallate (ECG) were increased, resulting in significant increase of antioxidant activity.

Conclusions

Although SE treatment could depolymerize PPC and produce CA/EC/ECG with high yield, it caused the yield loss of total procyanidins. SE treatment is a potential effective method to prepare procyanidins with low degree of polymerization and high antioxidant activity. However, it still needs to study further how to balance the yield of total procyanidins and catechin monomers (CA/EC/ECG).
CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis
Authors: Zhang Xiaohao , Lu Junying , Zhang Qinghua , Luo Qiang , Liu Bin
Published: March 2021

Background

Atherosclerosis (AS) is the most common type in cardiovascular disease. Due to its complex pathogenesis, the exact etiology of AS is unclear. circRNA has been shown to play an essential role in most diseases. However, the underlying mechanism of circRNA in AS has been not understood clearly.

Methods

Quantitative Real-Time PCR assay was used to detect the expression of circRSF1, miR-135b-5p and histone deacetylase 1 (HDAC1). Western blot was applied to the measure of protein expression of HDAC1, B-cell lymphoma-2 (Bcl-2), BCL2-associated X (Bax), cleaved-caspase-3, vascular cell adhesion molecule 1 (VCAM1), intercellular cell adhesion molecule-1 (ICAM1) and E-selectin. MTT assay and flow cytometry were used to detect cell proliferation and apoptosis, respectively. Dual luciferase reporter assay and RIP assay was used to determine the relationship among circRSF1, miR-135b-5p and HDAC1. Besides, an ELISA assay was performed to measure the levels of IL-1β, IL-6, TNF-α and IL-8.

Results

In this study, ox-LDL inhibited circRSF1 and HDAC1 expression while upregulated miR-135b-5p expression in Human umbilical vein endothelial cells (HUVECs). Importantly, ox-LDL could inhibit HUVECs growth. Moreover, promotion of circRSF1 or inhibition of miR-135b-5p induced cell proliferation while inhibited apoptosis and inflammation of ox-LDL-treated HUVECs, which was reversed by upregulating miR-135b-5p or downregulating HDCA1 in ox-LDL-treated HUVECs. More than that, we verified that circRSF1 directly targeted miR-135b-5p and HDAC1 was a target mRNA of miR-135b-5p in HUVECs.

Conclusion

CircRSF1 regulated ox-LDL-induced vascular endothelial cell proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in AS, providing new perspectives and methods for the treatment and diagnosis of AS.
Left atrial schwannoma in schwannomatosis: a case report
Authors: Yokoyama Kenji , Yoshizaki Tomoya , Tasaki Dai
Published: March 2021

Background

Primary cardiac schwannoma in the left atrium and schwannomatosis are rare diseases.

Case presentation

We report the case of a 46-year-old asymptomatic man who had tumor resection for parapharyngeal schwannoma at another institute 1 year ago. He was presented to our hospital for further evaluation of an abnormal cardiac shadow that was found incidentally. Computed tomography and transesophageal echocardiography revealed a cardiac tumor originating from the posterior wall of the left atrium, an atrial septal defect, and two other mediastinal tumors. The cardiac mass was completely excised with normal margins of the surrounding atrial wall. The post-resection defect and atrial septal defect were repaired using bovine pericardium. Pathological findings were compatible with benign schwannoma, and a diagnosis of schwannomatosis was made based on his medical history.

Conclusion

Primary cardiac schwannoma is an exceedingly rare tumor, and the incidence in schwannomatosis has not been reported in the literature.
Classification of pavement climatic regions through unsupervised and supervised machine learnings
Authors: Dong Qiao , Chen Xueqin , Dong Shi , Zhang Jun
Published: March 2021

This study extracted 16 climatic data variables including annual temperature, freeze thaw, precipitation, and snowfall conditions from the Long-term Pavement Performance (LTPP) program database to evaluate the climatic regionalization for pavement infrastructure. The effect and significance of climate change were firstly evaluated using time as the only predictor and t-test. It was found that both the temperature and humidity increased in most States. Around one third of the 800 weather stations record variation of freeze and precipitation classifications and a few of them show significant change of classifications over time based on the results of logistic regression analyses. Three unsupervised machine learning including Principle Component Analysis (PCA), factor analysis and cluster analysis were conducted to identify the main component and common factors for climatic variables, and then to classify datasets into different groups. Then, two supervised machine learning methods including Fisher’s discriminant analysis and Artificial Neural Networks (ANN) were adopted to predict the climatic regions based on climatic data. Results of PCA and factor analysis show that temperature and humidity are the first two principle components and common factors, accounting for 71.6% of the variance. The 4-means clusters include wet no freeze, dry no freeze, dry freeze and snow freeze. The best k-mean clustering suggested 9 clusters with more temperature clusters. Both the Fisher’s linear discriminant analysis and ANN can effectively predict climatic regions with multiple climatic variables. ANN performs better with higher R square and low misclassification rate, especially for those with more layers and nodes.
Do anti-carbamylated protein antibodies in rheumatoid arthritis reflect local and systemic osteoporosis? A study of osteoprotegrin and receptor activator for nuclear factor kappa B ligand and radiological assessment
Authors: Hussein Manal Shawky , Gaber Rasha Ahmed , Elsabagh Hala Mostafa , Rageh Elsayed Mohamed
Published: March 2021

Background

One of the most important and dangerous complications of rheumatoid arthritis (RA) is bone loss, which manifested by erosions and juxta-articular or systemic bone loss. Anti-carbamylated protein (anti-CarP) antibodies which are also called anti-homocitrulline antibodies have recently been found in RA. Increase anti-CarP antibody titres may lead to severe disease and increase the progression of bone loss. Osteoprotegrin and receptor activator for nuclear factor kappa B and its ligand (RANKL) are the main players in the pathogenesis of osteoporosis. Thus, we aimed to investigate and detect the presence and prevalence of anti-CarP in rheumatoid arthritis and their association with disease severity and osteoporosis, as well as with OPG/RANKL in 80 Egyptian RA patients to highlight this relationship which could be useful in managing RA patients with osteoporosis.

Results

Serum anti-CarP levels were significantly increased in the RA group compared with the control group (P< 0.001). We found a negative association between anti-CarP and anti-CCP and disease activity (r=−0.878, −0.534, respectively, P<0.001). We also found a positive correlation between anti-CarP and the Larsen score, DEXA score, RF, HAQ, and RANKL (r=0.646, 0.287, 0.243, 0.892, 0.671, 0.869 [respectively], P<0.001) and there was negative correlation between anti-CarP and OPG (r=−0.553, P<0.001).

Conclusion

Anti-CarP antibodies are associated with disease severity and disability in RA patients. They could play an important and significant role in the pathogenesis of osteoporosis in these patients.
Training, Validation, and Test of Deep Learning Models for Classification of Receptor Expressions in Breast Cancers From Mammograms
Authors: Ueda Daiju , Yamamoto Akira , Takashima Tsutomu , Onoda Naoyoshi , Noda Satoru , Kashiwagi Shinichiro , Morisaki Tamami , Honjo Takashi , Shimazaki Akitoshi , Miki Yukio
Published: March 2021

PURPOSE

The molecular subtype of breast cancer is an important component of establishing the appropriate treatment strategy. In clinical practice, molecular subtypes are determined by receptor expressions. In this study, we developed a model using deep learning to determine receptor expressions from mammograms.

METHODS

A developing data set and a test data set were generated from mammograms from the affected side of patients who were pathologically diagnosed with breast cancer from January 2006 through December 2016 and from January 2017 through December 2017, respectively. The developing data sets were used to train and validate the DL-based model with five-fold cross-validation for classifying expression of estrogen receptor (ER), progesterone receptor (PgR), and human epidermal growth factor receptor 2-neu (HER2). The area under the curves (AUCs) for each receptor were evaluated with the independent test data set.

RESULTS

The developing data set and the test data set included 1,448 images (997 ER-positive and 386 ER-negative, 641 PgR-positive and 695 PgR-negative, and 220 HER2-enriched and 1,109 non–HER2-enriched) and 225 images (176 ER-positive and 40 ER-negative, 101 PgR-positive and 117 PgR-negative, and 53 HER2-enriched and 165 non–HER2-enriched), respectively. The AUC of ER-positive or -negative in the test data set was 0.67 (0.58-0.76), the AUC of PgR-positive or -negative was 0.61 (0.53-0.68), and the AUC of HER2-enriched or non–HER2-enriched was 0.75 (0.68-0.82).

CONCLUSION

The DL-based model effectively classified the receptor expressions from the mammograms. Applying the DL-based model to predict breast cancer classification with a noninvasive approach would have additive value to patients.
Supercanonical transformations and time-dependent Hamiltonians
Authors: Anzaldo-Meneses A.
Published: March 2021

Starting from general self-adjoint linear combinations of generators of the superalgebra

a time-dependent Hamiltonian of a supersymmetric quantum mechanical system is defined by computing the supercommutator of the linear forms. The resulting Hamiltonian is given by the sum of two quadratic forms in odd, respectively even, generators of the superalgebra describing a class of systems containing bosons and fermions. Linear supercanonical transformations of wave vectors leave invariant a Heisenberg superalgebra and belong to the supergroup OSp

. The equations of motion for the supercanonical transformations in the Heisenberg picture are shown to be systems of ordinary differential equations. The unitary time evolution operator is constructed using the adjoint map. For periodic Hamiltonians, it is shown that this is a procedure to obtain effective Floquet Hamiltonians. The examples show that the known superalgebras-based approaches for the nuclear shell model and the Jaynes-Cummings model are incorporated in the lowest-dimensional cases. The presented approach opens the possibility to study quantum control problems defined by linear combinations of superalgebra generators with Grassmannian coefficients.
Multi-hole bands and quasi–two-dimensionality in Cr2Ge2Te6 studied by angle-resolved photoemission spectroscopy
Authors: Yilmaz T. , Geilhufe R. M. , Pletikosić I. , Fernando G. W. , Cava R. J. , Valla T. , Vescovo E. , Sinkovic B.
Published: March 2021

In the present work, we investigate the electronic structure of the two-dimensional ferromagnet Cr2Ge2Te6 by photoemission spectroscopy and ab initio calculations. Our results demonstrate the presence of multi-hole–type bands in the vicinity of the Fermi level indicating that the material can support high electrical conductivity by manipulating the chemical potential. Also, our photon energy-dependent angle-resolved photoemission experiment shows that several of the hole bands exhibit weak dispersion with varied incident photon energy providing experimental signature for the two-dimensional nature of Cr2Ge2Te6. Thereby, these findings can pave the way for further studies towards the application of Cr2Ge2Te6 in electronic devices.
Exercise in Pregnancy Increases Placental Angiogenin without Changes in Oxidative or Endoplasmic Reticulum Stress
Authors: HARDY DANIEL B. , MU XIN , MARCHIORI KATELYN S. , MOTTOLA MICHELLE F.
Published: March 2021

Purpose

Despite immense research highlighting maternal–fetal benefits of exercise during pregnancy, there remain concerns that exercise may undermine placental function. Although maternal exercise has demonstrated favorable aerobic conditioning responses in the mother, it is not known whether maternal exercise promotes increased angiogenesis in the placenta, perhaps at the expense of impaired endoplasmic reticulum (ER) homeostasis and/or oxidative stress. We investigated if a mild (30% heart rate reserve) and/or moderate (70% heart rate reserve) exercise regime in healthy pregnant women affected placental markers of angiogenesis, ER stress, and oxidative stress. We hypothesized that the improved aerobic conditioning of mothers who exercise is beneficial to enhance placental angiogenesis and normal maternal–fetal outcomes.

Methods

Placental tissues were collected within 1 h of delivery from a convenience sample of 29 healthy mothers of full-term infants. Twenty-one women participated in routine exercise from midgestation (16–20 wk) until term of either mild or moderate intensity, whereas eight sedentary women served as controls.

Results

No differences were identified between groups including gestational length, fetal–placental weight ratio, or APGAR scoring. All exercisers exhibited a significant 20-fold increase in the mRNA (as assessed by quantitative real-time polymerase chain reaction) and a 10-fold increase in the protein expression of angiogenin (e.g., ANG1) in the placenta. However, in both exercising groups, no increases in placental markers (i.e., HIF1α, VEGF), ER stress (i.e., spliced XBP1, ATF4, ATF6, CHOP, and BAX), or oxidative stress (i.e., SOD1, SOD2) were observed.

Conclusions

Overall, our study suggests that mild- and moderate-intensity exercise increases angiogenesis but does not increase placental oxidative or ER stress in healthy pregnancies, bolstering support for routine exercise as a part of standard care in pregnant women. Future studies are warranted to investigate the potential benefits of exercise on ANG1 in pathological pregnancies.
Blood Pressure Responses to Static and Dynamic Knee Extensor Exercise between Sexes: Role of Absolute Contraction Intensity
Authors: LEE JORDAN B. , LUTZ WILLIAM , OMAZIC LUCAS J. , JORDAN MITCHELL A. , CACOILO JOSEPH , GARLAND MATTHEW , POWER GEOFFREY A. , MILLAR PHILIP J.
Published: March 2021

Purpose

Males have larger blood pressure (BP) responses to relative-intensity static handgrip exercise compared with females. Controlling for absolute load (maximal voluntary contraction (MVC)) abolishes these differences. Whether similar observations exist during large muscle mass exercise or dynamic contractions, and the mechanisms involved, remains unknown.

Methods

BP, heart rate, muscle oxygenation (near-infrared spectroscopy), and rectus femoris EMG were recorded in 28 males and 17 females during 10% and 30% MVC static (120 s) and isokinetic dynamic (180 s; 1:2 work-to-rest ratio; angular velocity, 60°·s−1) knee extensor exercise. Static and dynamic exercises were completed on separate visits, in a randomized order. Sex differences were examined with and without statistical adjustment of MVC (ANCOVA).

Results

Males had larger systolic BP responses (interaction, P < 0.0001) and muscle deoxygenation (interaction, P < 0.01) than did females during 10% static exercise, with no difference in EMG (interaction, P = 0.67). Peak systolic BP was correlated with MVC (r = 0.55, P = 0. 0001), and adjustment for MVC abolished sex differences in systolic BP (interaction, P = 0.3). BP, heart rate, muscle oxygenation/deoxygenation, and EMG responses were similar between sexes during 30% static exercise (interaction; all, P > 0.2), including following adjustment for MVC (all, P > 0.1). Males had larger systolic BP responses during dynamic exercise at 10% and 30% (interaction; both, P = 0.01), which were abolished after adjustment for MVC (interaction; both, P > 0.08). Systolic BP responses were correlated with absolute MVC and stroke volume responses during 10% (r = 0.31, P = 0.04; r = 0.61, P < 0.0001, respectively) and 30% (r = 0.48, P = 0.001; r = 0.59, P < 0.0001, respectively).

Conclusions

Absolute contraction intensity can influence systolic BP responses to 10% but not 30% MVC static, as well as 10% and 30% MVC dynamic knee extensor exercise, and should be considered in cross-sectional comparisons of exercise BP.