bluuebunny/crossref_metadata_2025 huggingface dataset:
Relation between Strawberry Fruit Redness and Bioactivity: Deciphering the Role of Anthocyanins as Health Promoting Compounds
Authors: Martínez-Ferri Elsa , Forbes-Hernandez Tamara Yuliet , Cervantes Lucía , Soria Carmen , Battino Maurizio , Ariza María Teresa
Published: December 2023

The red colour of most berries is often associated to fruit healthiness, since it has been linked to enrichment in anthocyanins (polyphenol with antioxidative properties). However, recent studies suggest that anthocyanins could not be the major contributors to bioactivity leading to uncertainty about their role as important molecules in the generation of health-promoting properties. To shed light on this issue, spectrophotometric and HPLC techniques were used for characterizing the content of phenolic compounds, including anthocyanins, in fruits of red (Fragaria x ananassa, cv. Fortuna) and white strawberry (Fragaria vesca spp. XXVIII) species (distinguishing receptacle from achene). In addition, the effect of these extracts on the reduction of intracellular ROS was tested, as well as on the activity of antioxidant enzymes and the quantification of cell oxidation markers. The results showed that white receptacle extracts (deprived of anthocyanins) were able to protect cells from oxidative damage to a greater extent than red fruits. This could be due per se to their high antioxidant capacity, greater than that shown in red fruits, or to the ability of antioxidants to modulate the activity of antioxidant enzymes, thus questioning the positive effect of anthocyanins on the wholesomeness of strawberry fruits. The results shed light on the relevance of anthocyanins in the prevention of health-associated oxidative damage.
Exploring the Bioactive Potential of Calostoma insigne, an Endangered Culinary Puffball Mushroom, from Northeastern Thailand
Authors: Saengha Worachot , Karirat Thipphiya , Pitisin Nathanon , Plangklang Supawadee , Butkhup Luchai , Udomwong Piyachat , Ma Nyuk Ling , Konsue Ampa , Chanthaket Pornwipa , Katisart Teeraporn , Luang-In Vijitra
Published: December 2023

Calostoma insigne puffball mushrooms are only found in forests with rich biodiversity in very few countries including Thailand, and their biofunctions remain largely unexplored. This study used the agar disk diffusion assay, the anti-glucosidase assay, and the 3, 4, 5-dimethylthiazol-2-yl-2-5-diphenyltetrazolium bromide (MTT) assay to evaluate the bioactive potential of these endangered puffball mushrooms. Internal transcribed spacer (ITS) gene analysis identified C. insigne, a puffball mushroom with green, globose, and spiny spores. Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the polysaccharide structure while scanning electron microscopy (SEM) revealed a fiber-like network. The ethanolic gelatinous fruiting body extract exhibited 1,1-diphenyl-2-picrylhydrazyl (DPPH)-scavenging capacity (57.96%), a ferric ion-reducing antioxidant power (FRAP) value of 1.73 mg FeSO4/g, and α-glucosidase inhibition (73.18%). C. insigne cytotoxicity was effective towards HT-29 colon cancer cells using the MTT assay (IC50 of 770.6 µg/mL at 72 h) and also showed antiproliferative capacity (IC50 of 297.1 µg/mL). This puffball mushroom stimulated apoptotic genes and proteins (caspase-3, Bax, and p21) via an intrinsic apoptotic pathway in HT-29 cells. In the laboratory, the medium formula consisting of 20% potato, 2% sucrose, and 0.2% peptone was optimal to increase fungal mycelial biomass (2.74 g DW/100 mL), with propagation at pH 5.0 and 30 °C. Puffball mushrooms are consumed as local foods and also confer several potential health benefits, making them worthy of conservation for sustainable utilization.
Citrus Peel Extracts: Effective Inhibitors of Heterocyclic Amines and Advanced Glycation End Products in Grilled Pork Meat Patties
Authors: Xu Yang , Li Guangyu , Mo Lan , Li Maiquan , Luo Jie , Shen Qingwu , Quan Wei
Published: December 2023

In the present study, citrus peels were extracted using various conventional and deep eutectic solvents (DESs). Compared to other citrus peel extracts, the DES extract based on choline chloride showed notably higher total phenolic and flavonoid content levels, along with superior antioxidant activity, among these extracts. Consequently, this study aimed to further investigate the inhibitory effects of the choline chloride based DES extract on the production of both free and bound heterocyclic amines (HAs) and advanced glycation end products (AGEs) in roast pork meat patties. The results indicated that the addition of choline chloride-based DES extracts, particularly the choline chloride-carbamide based DES extract, can effectively reduce the oxidation of lipids and proteins by quenching free radicals. This approach proves to be the most efficient in reducing the formation of both HAs and AGEs, leading to a significant reduction of 19.1–68.3% and 11.5–66.5% in free and protein-bound HAs, respectively. Moreover, the levels of free and protein-bound AGEs were reduced by 50.8–50.8% and 30.5–39.8%, respectively, compared to the control group. Furthermore, the major phenolics of citrus peel extract identified by UHPLC-MS were polymethoxylated flavonoids (PMFs) including hesperidin, isosinensetin, sinensetin, tetramethoxyflavone, tangeretin, and hexamethoxyflavone, which inferring that these compounds may be the main active ingredients responsible for the antioxidant activity and inhibition effects on the formation of HAs and AGEs. Further research is needed to explore the inhibitory effects and mechanisms of PMFs with different chemical structures on the formation of HAs and AGEs.
Grey Time Power Model with Caputo Fractional Derivative
Authors: Hu Pan , Gu Chuan-Yun
Published: December 2023

In this paper, the inverse cumulative grey time power model with a Caputo fractional derivative is established, and the solution to the whitening equation is given by the Laplace transform. To improve the prediction accuracy of the model, the linear-tangent-function transformation is used to improve the smoothness of the data sequence, and a grey time power model is obtained, which has higher accuracy than the sinusoidal-function transformation, negative-exponential-function transformation and logarithmic-function transformation. The form and application range of the model are generalized.
Hybrid Materials of Bio-Based Aerogels for Sustainable Packaging Solutions
Authors: Vrabič-Brodnjak Urška
Published: December 2023

This review explores the field of hybrid materials in the context of bio-based aerogels for the development of sustainable packaging solutions. Increasing global concern over environmental degradation and the growing demand for environmentally friendly alternatives to conventional packaging materials have led to a growing interest in the synthesis and application of bio-based aerogels. These aerogels, which are derived from renewable resources such as biopolymers and biomass, have unique properties such as a lightweight structure, excellent thermal insulation, and biodegradability. The manuscript addresses the innovative integration of bio-based aerogels with various other materials such as nanoparticles, polymers, and additives to improve their mechanical, barrier, and functional properties for packaging applications. It critically analyzes recent advances in hybridization strategies and highlights their impact on the overall performance and sustainability of packaging materials. In addition, the article identifies the key challenges and future prospects associated with the development and commercialization of hybrid bio-based aerogel packaging materials. The synthesis of this knowledge is intended to contribute to ongoing efforts to create environmentally friendly alternatives that address the current problems associated with conventional packaging while promoting a deeper understanding of the potential of hybrid materials for sustainable packaging solutions.
Fabrication of Tough Double-Network Hydrogels from Highly Cross-Linked Brittle Neutral Networks Using Alkaline Hydrolysis
Authors: Shams Es-haghi S. , Weiss R. A.
Published: December 2023

This paper describes a simple method to synthesize tough hydrogels from a highly cross-linked neutral network. It was found that applying alkaline hydrolysis to a highly cross-linked hydrogel synthesized from acrylamide (AAm) can increase its swelling ratio dramatically. Double-network (DN) hydrogels synthesized from polymerization of loosely cross-linked AAm networks inside a highly cross-linked AAm gel were not tough. However, repeating the same recipes with a second polymerization step to synthesize a DN hydrogel from a hydrolyzed highly cross-linked AAm gel resulted in tough hydrogels. Those gels exhibited finite tensile behavior similar to that of conventional DN hydrogels. Moreover, craze-like patterns were observed during tensile loading of a DN hydrogel synthesized from a hydrolyzed highly cross-linked first network and a loosely cross-linked second network. The patterns remained in the gel even after strain hardening at high stretch ratios. The craze-like pattern formation was suppressed by increasing the concentration of cross-linking monomer in the second polymerization step. Crack propagation in DN hydrogels synthesized using hydrolysis was also studied by applying a tensile load on notched specimens.
Amateur Family Genealogists Researching Their Family History: A Scoping Review of Motivations and Psychosocial Impacts
Authors: Mitchell Barbara A. , Kim Boah
Published: December 2023

A rapidly rising number of people are engaging in family genealogical research and have purchased home-based DNA testing kits due to increased access to online resources and consumer products. The purpose of this systematic scoping review is to identify and elucidate the motivations (i.e., pathways, reasons for conducting family history research) and the consequences (i.e., psychosocial impacts) of participating in this activity by amateur (unpaid) family genealogists. Studies published from January 2000 to June 2023 were included in our review, using the PRISMA methodology outlined by the Joanna Briggs Institute’s (JBI) Reviewer Manual. A total of 1986 studies were identified using selected keywords and electronic databases. A full-text review was conducted of 73 studies, 26 of which met our eligibility criteria. The multiple dominant themes that emerged from the data analysis are organized into five categories: (1) the motivations for practicing family history research, (2) emotional responses to family secrets and previously unknown truths, (3) impacts on relationship with the family of origin and other relatives, (4) impacts on personal identity (including ethnic/racialized and family/social), and (5) identity exploration and reconstruction. Finally, these themes are connected to broader theoretical/conceptual linkages, and further, an agenda for future research inquiry is developed.
Geogrid-Enhanced Modulus and Stress Distribution in Clay Soil
Authors: Chen Qiming
Published: December 2023

A series of laboratory and large-scale field model footing tests were conducted to assess the modulus and stress distribution behavior of a clayey soil foundation, both with/without geogrid reinforcement, deviating from the conventional approach of evaluating the strength performance, such as bearing capacity. The modulus was evaluated at three settlement ratios of s/B = 1, 3, and 5%, while the stress distribution angle α was estimated at three applied surface pressures of 234 kPa, 468 kPa, and 936 kPa. The results indicated a stiffer load-settlement response when geogrid reinforcement was included. The modulus of reinforced clayey soil remained nearly constant for test sections with the same reinforced ratio, with the modulus improvement increasing as the reinforced ratio (Rr) increased. The modulus improvement also increased with the settlement ratio (s/B). These results demonstrated that the stress distribution improvement decreased as the surface pressure increased. Generally, both the modulus and stress distribution improvement exhibited an increase with an increase in the tensile modulus of the geogrid. While laboratory model tests consistently provided a higher improvement in the modulus than large-scale field model tests in this study due to a higher reinforced ratio, the stress distribution improvement was similar for both.
Perception of Paternal Postpartum Depression among Healthcare Professionals: A Qualitative Study
Authors: Essadek Aziz , Marie Alix , Rioux Michel-Alexandre , Corruble Emmanuelle , Gressier Florence
Published: December 2023

The pathway to parenthood constitutes a fundamental and transformative stage in every individual’s life. While postpartum depression in mothers has been increasingly studied and acknowledged, paternal postpartum depression (PPD) has garnered only moderate research attention. This study aims to delve into the comprehension and knowledge of healthcare professionals who may encounter men suffering from postpartum depression. Within the framework of this qualitative research, we conducted six semi-structured interviews with various healthcare professionals. The data were subjected to interpretative phenomenological analysis, revealing the following themes: (1) the professionals’ uncertainty in the face of paternal PPD; (2) the context and timing of healthcare professionals’ involvement appeared unsuited for detecting paternal PPD; (3) the experiences of fathers were found not to be shared with healthcare professionals due to their inhibitions and avoidance reactions; (4) the social representation of the role of fathers influenced professionals in their considerations of this aspect. Strengthening the training and confidence of healthcare professionals in France would lead to an enhancement in the screening and management of paternal PPD. Additionally, the healthcare system should better organize postnatal support to enable caregivers to be more available during the peak of depression occurrence.
Wellness Perception of South Korean Elementary School Students during the COVID-19 Endemic
Authors: Seo Yongsuk , Lee Eui-Jae , Kim Jin-Young , Yoo Jung In , Youn Hyun-su
Published: December 2023

This study aimed to analyze health management awareness among South Korean elementary school students in COVID-19 endemic areas. Methods. Using convenience sampling, 675 South Korean elementary school students (age 11–12 years old) were selected as participants in July 2023. Data for the study were collected via online and offline surveys between July and August 2023. The collected data were subjected to frequency, reliability, and multicollinearity analyses, independent sample t-tests, and importance-performance analysis (IPA). Results. The findings indicated the following: (1) There was no significant difference in health management performance between male and female children. (2) Children who had not experienced COVID-19 infection, had a higher level of “hygiene management” performance. (3) Among children who did not wear masks during physical activity, “mental health management” and “physical activity management” performance were higher, while “hygiene management” performance was lower. (4) The IPA matrix analysis revealed that, compared to the COVID-19 pandemic period, “physical activity management”, “dietary habit management”, and “sleep management” still required improvement, while “hygiene management” and “disease management” appeared to have decreased due to the relaxation of epidemic control efforts. Conclusion. As per the study’s findings, schools, local communities, and families should make efforts to develop and implement preventive and individualized health management programs that consider the individual characteristics of their children.
Whole-Body Cryostimulation in Functional Neurological Disorders: A Case Report
Authors: Verme Federica , Fontana Jacopo Maria , Piterà Paolo , Alito Angelo , Saffioti Silvia , Baccalaro Gabriele , Zebellin Giuliano , Capodaglio Paolo
Published: December 2023

Functional neurological disorders (FNDs) are complex disabling conditions requiring a multiple rehabilitation intervention. Here, we propose a new use of whole-body cryostimulation (WBC) that was implemented in a multidisciplinary rehabilitation programme in a wheelchair-ridden woman diagnosed with FND and other comorbidities. WBC is a promising adjuvant treatment in various conditions of rehabilitation interest, mainly because of its wide range of rapid effects, from anti-inflammatory to pain and autonomic modulating effects. The 4-week program included physiotherapy, nutritional intervention, psychological support, and WBC (−110 °C for 2 min). Questionnaires to assess disease impact, pain level, fatigue and sleep quality were administered. At discharge, improvements in body composition, haematological biomarkers, physical performance, and questionnaire scores were observed. The patient was able to walk independently with a walker for medium distances and reported unprecedented improvements, particularly in functional parameters and questionnaire scores. Although the extent to which WBC per se contributed to the measured improvements cannot be ascertained, subjective reports and our clinical observations indicate that WBC, the only intervention not previously experienced by the patient, acted as a booster for the rehabilitation interventions. Further research will be necessary to rule out any possible placebo effect and to confirm the effects of WBC on FND.
Self-Reported Health Inequalities among Older Adults in Saudi Arabia
Authors: Al-Hanawi Mohammed Khaled
Published: December 2023

Considering the rising life expectancy, the growing population of older adults poses challenges in providing adequate healthcare services. Self-reported health is an important indicator of overall health, predicting morbidity and mortality. This study investigated self-reported health inequalities among older adults in Saudi Arabia and the underlying factors contributing to establishing such inequalities. The study utilized data from the 2018 Saudi Family Health Survey, focusing on 2023 respondents aged ≥60 years with complete data. Univariate, bivariate, and multivariate logistic regression analyses were employed to explore socio-economic factors linked to health inequalities. Additionally, concentration curves and indices were used to assess the magnitude of health inequalities among older adults. The findings indicate a higher prevalence of self-reported poor health among respondents aged ≥70 years and those with chronic diseases. Age, education, income level, marital status, and insurance coverage were other factors significantly linked to reporting poor health. Inequality analysis revealed a concentration of poor health among less educated individuals (concentration index = −0.261, p < 0.01). Both income- and education-based indices highlighted a concentration of poor health among men with lower income and education levels. Addressing healthcare inequalities among older adults requires targeted policy efforts, focusing on those aged ≥70, unmarried individuals, those without insurance coverage, those with chronic illnesses, and those with lower education levels. Targeted interventions for these groups can address their unique healthcare needs and promote equitable health outcomes.
Use of LiDAR Technology for the Study and Analysis of Construction Phases and Deformations in the Gothic Church of Biar (Spain)
Authors: Huesca-Tortosa Jose Antonio , Spairani-Berrio Yolanda , Saura-Gómez Pascual
Published: December 2023

This work provides new data on the church of Nuestra Señora de la Asunción in Biar, combining the investigation of documentary sources with the analysis of the geometry obtained using LiDAR technology and the study of stone materials. This monument has a Proto-Renaissance façade of great architectural value, as it was the first building in the province of Alicante, in Spain, to use Renaissance decorations at the beginning of the 16th century. Its main façade reflects the four centuries of its construction from the 15th to the 18th centuries. The building has been digitised using LiDAR technology and photogrammetry. The graphic representation of the point cloud obtained from the aforementioned techniques makes it possible to study deformations in colour gradient with respect to a reference plane. The results obtained after the analysis of these data show that the method used in this work has served to detect and corroborate the constructive evolutions of the church obtained from the documentary sources investigated. This work serves as an example for similar works, proposing the incorporation of the analysis of anomalies in the geometry of the facings as a new variable that should be considered to complement the rest of the usual studies, bringing to light deformations and irregularities that at first sight may go unnoticed.
Viticultural Climate Indexes and Their Role in The Prediction of Anthocyanins and Other Flavonoids Content in Seedless Table Grapes
Authors: Crupi Pasquale , Alba Vittorio , Gentilesco Giovanni , Gasparro Marica , Ferrara Giuseppe , Mazzeo Andrea , Coletta Antonio
Published: December 2023

Background: Viticulture bioclimatic indexes like the Heliothermal Index (HI), Cool Night Index (CI), and Dryness Index (DI), can be used to assess the influence of climate on grapes’ quality. Methods: HI, CI, and DI + total seasonal irrigation were utilized to assess the effect of climate on the flavonoids content and composition of two Vitis vinifera seedless varieties, ‘Summer Royal’ and ‘Crimson Seedless’, both grown in Apulia (Southern Italy). Results: The flavonoids content was significantly affected by variety and climate conditions on the base of HI, CI, and DI + total seasonal irrigation. Factor analysis applied to climate indexes and flavonoids showed that anthocyanins and flavonols were negatively and positively correlated to CI in both varieties, respectively. Additionally, warmer night temperatures determined higher fla-van-3-ols. HI increase promoted anthocyanins, flavonols, and flavan-3-ols content in Crimson Seedless, whilst it induced an opposite trend in Summer Royal. Finally, DI + total seasonal irrigation showed to be positively linked to flavonols content and negatively linked to anthocyanins content just in the case of Crimson Seedless. Significant regression models were also determined between climate indexes and productive parameters (i.e., yield, TSS, TA, pH, bunch, and berry weight). Conclusions: Climate indexes HI, CI, and DI + total seasonal irrigation showed an effect on quality grape parameters like flavonoids and contributed to building predictive models when new climatic zones are going to be evaluated for the production of table grapes.
Insights from a Patent Portfolio Analysis on Sensor Technologies for Measuring Fruit Properties
Authors: Kevrešan Žarko , Mastilović Jasna , Kukolj Dragan , Ubiparip Samek Dragana , Kovač Renata , Đerić Marina , Bajić Aleksandra , Ostojić Gordana , Stankovski Stevan
Published: December 2023

A patent portfolio focusing on sensors for the measurement of fruit properties was generated and analyzed with the aim of contributing to a better understanding of the trends in the development and application of sensors intended for measuring fruit properties and their changes. A patent portfolio of 189 patents, utility models and patent applications was formed. Three groups of patents were identified: (i) sensor-based measurement of individual parameters, (ii) multisensor solutions for the simultaneous monitoring of multiple relevant aspects and (iii) solutions integrating sensor-derived data with artificial intelligence tools and techniques. The analysis of the patent portfolio pointed out the main driving forces of technology strengthening in the field of fruit property measurement. The development of sensing technologies enables the real-time, rapid and cost-effective determination of ever-increasing and more sophisticated sets of fruit properties and environmental conditions. Solutions integrating different sensing technologies into multisensor systems for monitoring fruit quality, ripening or freshness as holistic concepts opens avenues for the introduction of a new approach to fresh produce management. Increasing numbers of solutions introducing the application of artificial intelligence tools such as computer vision, machine learning and deep learning into the fresh produce supply chain contribute to the possibilities of substituting human decision-making at points of relevance for fresh produce management with optimal evidence-based solutions.
A Scoping Review of Urban Planning Decision Support Tools and Processes That Account for the Health, Environment, and Economic Benefits of Trees and Greenspace
Authors: Tefera Yonatal , Soebarto Veronica , Bishop Courtney , Kandulu John , Williams Carmel
Published: December 2023

Compelling evidence shows that trees and greenspaces positively impact human well-being and the environment and offer economic benefits. Nevertheless, there exists a knowledge gap regarding the extent to which this evidence is efficiently incorporated into existing urban planning decision-making processes. This scoping review identified the extent to which urban planning decision-making frameworks, models, and tools consider the health, environmental, and economic benefits of trees and greenspace. Out of 28 reviewed studies, 11 (39%) reported on frameworks, models, and tools that take into account the health, environmental, and economic dimensions of trees and greenspace. Additionally, seven studies provided comprehensive coverage of at least one of the three key dimensions. However, none of the decision support frameworks, models, or tools comprehensively integrated all three dimensions, with only two tools (7%) scoring above 50% (five or more out of nine) in terms of comprehensiveness. This review highlights the urgent need to incorporate the true economic and monetary values of the health and environmental benefits of trees and greenspace to inform urban development decision making.
Silicon-Selenium Interplay Imparts Cadmium Resistance in Wheat through an Up-Regulating Antioxidant System
Authors: Manzoor Maria , Abdalla Muna Ali , Hussain Md Arif , Mühling Karl Hermann
Published: December 2023

Cadmium (Cd), being a highly toxic heavy metal, significantly impacts plant growth and development by altering nutrient uptake and causing oxidative and structural damage, resulting in reduced yield. To combat Cd toxicity and accumulation in wheat, it was hypothesized that co-application of Selenium (Se) and Silicon (Si) can reduce the adverse effect of Cd and regulate Cd resistance while improving Se fortification in wheat. Therefore, this study evaluated the comparative effect of Se and Si on the growth and antioxidant defense systems of wheat plants grown in a hydroponic setup. Briefly, the plants were acclimatized to the hydroponic solution for 1 week and then exposed to 10 µmol Cd. Afterwards, the treatments, including 0.2 mmol Si and 1.5 µmol Se, were applied as a root and foliar application, respectively. Plants supplemented with both Se and Si showed improved biomass and other physiological growth attributes, and this response was associated with improved activity/contents of antioxidants, including glutathione (GSH) content, glutathione reductase (GR), ascorbate peroxidase (APX), and catalase (CAT), with related lowering of hydrogen peroxide, malondialdehyde content, and structural damages. Moreover, by Se + Si supplementation, a decrease in total S levels in plant tissues was observed, whereas an increase in total protein concentration and GSH indicated a different and novel mechanism of Cd tolerance and S homeostasis in the plant. It was observed that Si was more involved in significantly reducing Cd translocation by stabilizing Cd in the root and reducing its content in the soluble fraction in both the root and shoot. Whereas Se was found to play the main role in reducing the oxidative damage caused by Cd, and the effect was more profound in the shoot. In addition, this study also observed a positive correlation between Si and Se for relative uptake, which had not been reported earlier. Our findings show that the Se and Si doses together benefit growth regulation and nutrient uptake; additionally, their combinations support the Cd resistance mechanism in wheat through upregulation of the antioxidant system and control of Cd translocation and subcellular distribution, ultimately contributing to the nutritional quality of wheat produced. Thus, it is concluded that the co-application of Se and Si has improved the nutritional quality while reducing the Cd risk in wheat and therefore needs to be employed as a potential strategy to ensure food safety in a Cd-contaminated environment.
MS Identification of Blood Plasma Proteins Concentrated on a Photocrosslinker-Modified Surface
Authors: Gordeeva Arina I. , Valueva Anastasia A. , Rybakova Elizaveta E. , Ershova Maria O. , Shumov Ivan D. , Kozlov Andrey F. , Ziborov Vadim S. , Kozlova Anna S. , Zgoda Victor G. , Ivanov Yuri D. , Ilgisonis Ekaterina V. , Kiseleva Olga I. , Ponomarenko Elena A. , Lisitsa Andrey V. , Archakov Alexander I. , Pleshakova Tatyana O.
Published: December 2023

This work demonstrates the use of a modified mica to concentrate proteins, which is required for proteomic profiling of blood plasma by mass spectrometry (MS). The surface of mica substrates, which are routinely used in atomic force microscopy (AFM), was modified with a photocrosslinker to allow “irreversible” binding of proteins via covalent bond formation. This modified substrate was called the AFM chip. This study aimed to determine the role of the surface and crosslinker in the efficient concentration of various types of proteins in plasma over a wide concentration range. The substrate surface was modified with a 4-benzoylbenzoic acid N-succinimidyl ester (SuccBB) photocrosslinker, activated by UV irradiation. AFM chips were incubated with plasma samples from a healthy volunteer at various dilution ratios (102X, 104X, and 106X). Control experiments were performed without UV irradiation to evaluate the contribution of physical protein adsorption to the concentration efficiency. AFM imaging confirmed the presence of protein layers on the chip surface after incubation with the samples. MS analysis of different samples indicated that the proteomic profile of the AFM-visualized layers contained common and unique proteins. In the working series of experiments, 228 proteins were identified on the chip surface for all samples, and 21 proteins were not identified in the control series. In the control series, a total of 220 proteins were identified on the chip surface, seven of which were not found in the working series. In plasma samples at various dilution ratios, a total of 146 proteins were identified without the concentration step, while 17 proteins were not detected in the series using AFM chips. The introduction of a concentration step using AFM chips allowed us to identify more proteins than in plasma samples without this step. We found that AFM chips with a modified surface facilitate the efficient concentration of proteins owing to the adsorption factor and the formation of covalent bonds between the proteins and the chip surface. The results of our study can be applied in the development of highly sensitive analytical systems for determining the complete composition of the plasma proteome.
Loop-Mediated Isothermal Amplification of DNA (LAMP) as an Alternative Method for Determining Bacteria in Wound Infections
Authors: Gieroń Monika , Żarnowiec Paulina , Zegadło Katarzyna , Gmiter Dawid , Czerwonka Grzegorz , Kaca Wiesław , Kręcisz Beata
Published: December 2023

The increasing number of patients with chronic wounds requires the development of quick and accurate diagnostics methods. One of the key and challenging aspects of treating ulcers is to control wound infection. Early detection of infection is essential for the application of suitable treatment methods, such as systemic antibiotics or other antimicrobial agents. Clinically, the most frequently used method for detecting microorganisms in wounds is through a swab and culture on appropriate media. This test has major limitations, such as the long bacterial growth time and the selectivity of bacterial growth. This article presents an overview of molecular methods for detecting bacteria in wounds, including real-time polymerase chain reaction (rtPCR), quantitative polymerase chain reaction (qPCR), genotyping, next-generation sequencing (NGS), and loop-mediated isothermal amplification (LAMP). We focus on the LAMP method, which has not yet been widely used to detect bacteria in wounds, but it is an interesting alternative to conventional detection methods. LAMP does not require additional complicated equipment and provides the fastest detection time for microorganisms (approx. 30 min reaction). It also allows the use of many pairs of primers in one reaction and determination of up to 15 organisms in one sample. Isothermal amplification of DNA is currently the easiest and most economical method for microbial detection in wound infection. Direct visualization of the reaction with dyes, along with omitting DNA isolation, has increased the potential use of this method.
Antileishmanial Activity, Toxicity and Mechanism of Action of Complexes of Sodium Usnate with Lanthanide Ions: Eu(III), Sm(III), Gd(III), Nd(III), La(III) and Tb(III)
Authors: da Silva Fernanda , Rizk Yasmin Silva , das Neves Amarith Rodrigues , Lourenço Estela Mariana Guimarães , Ferreira Alda Maria Teixeira , Monteiro Melquisedeque Mateus , de Lima Dênis Pires , Perdomo Renata Trentin , Bonfá Iluska Senna , Toffoli-Kadri Mônica Cristina , Duarte Adriana Pereira , Nunes Daniel Mendes , Martines Marco Antonio Utrera , Piranda Eliane Mattos , de Arruda Carla Cardozo Pinto
Published: December 2023

Leishmaniases are neglected diseases with limited therapeutic options. Diffuse cutaneous leishmaniasis can occur in Brazil due to Leishmania amazonensis. This study details the antileishmanial activity and cytotoxicity of complexes of sodium usnate (SAU) with lanthanide ions ([LnL3 (H2O)x] (Ln = La(III), Nd(III), Gd(III), Tb(III), Eu(III) and Sm(III); L = SAU). All lanthanide complexes were highly active and more potent than SAU against L. amazonensis promastigotes and intracellular amastigotes (Pro: IC50 < 1.50 μM; Ama: IC50 < 7.52 μM). EuL3·3H2O and NdL3·3H2O were the most selective and effective on intracellular amastigotes, with a selectivity index of approximately 7.0. In silico predictions showed no evidence of mutagenicity, tumorigenicity or irritation for all complexes. Treatment with EuL3·3H2O triggered NO release even at the lowest concentration, indicating NO production as a mechanism of action against the parasite. Incubating promastigotes with the lanthanide complexes, particularly with SmL3·4H2O and GdL3·3H2O, led to a change in the mitochondrial membrane potential, indicating the ability of these complexes to target this essential organelle. The same complexes caused cell death through cell membrane disruption, but their relationship with early or late apoptotic processes remains unclear. Thus, the inclusion of lanthanide ions in SAU improves selectivity with a promising mechanism of action targeting the mitochondria.
Exogenous IL-17A Alleviates Social Behavior Deficits and Increases Neurogenesis in a Murine Model of Autism Spectrum Disorders
Authors: Willinger Yehoshua , Friedland Cohen Daniella R. , Turgeman Gadi
Published: December 2023

Among the proposed mechanisms for autism spectrum disorders (ASD) is immune dysregulation. The proinflammatory cytokine Interleukine-17A (IL-17A) was shown to play a key role in mediating immune-related neurodevelopmental impairment of social behavior. Nevertheless, post-developmental administration of IL-17A was found to increase social behavior. In the present study, we explored the effect of post-developmental administration of IL-17A on ASD-like behaviors induced by developmental exposure to valproic acid (VPA) at postnatal day 4. At the age of seven weeks, VPA-exposed mice were intravenously injected twice with recombinant murine IL-17A (8 μg), and a week later, they were assessed for ASD-like behavior. IL-17A administration increased social behavior and alleviated the ASD-like phenotype. Behavioral changes were associated with increased serum levels of IL-17 and Th17-related cytokines. Exogenous IL-17A also increased neuritogenesis in the dendritic tree of doublecortin-expressing newly formed neurons in the dentate gyrus. Interestingly, the effect of IL-17A on neuritogenesis was more noticeable in females than in males, suggesting a sex-dependent effect of IL-17A. In conclusion, our study suggests a complex role for IL-17A in ASD. While contributing to its pathology at the developmental stage, IL-17 may also promote the alleviation of behavioral deficits post-developmentally by promoting neuritogenesis and synaptogenesis in the dentate gyrus.
Collagen-like Osteoclast-Associated Receptor (OSCAR)-Binding Motifs Show a Co-Stimulatory Effect on Osteoclastogenesis in a Peptide Hydrogel System
Authors: Vitale Mattia , Ligorio Cosimo , Richardson Stephen M. , Hoyland Judith A. , Bella Jordi
Published: December 2023

Osteoclastogenesis, one of the dynamic pathways underlying bone remodelling, is a complex process that includes many stages. This complexity, while offering a wealth of therapeutic opportunities, represents a substantial challenge in unravelling the underlying mechanisms. As such, there is a high demand for robust model systems to understand osteoclastogenesis. Hydrogels seeded with osteoclast precursors and decorated with peptides or proteins mimicking bone’s extracellular matrix could provide a useful synthetic tool to study pre-osteoclast-matrix interactions and their effect on osteoclastogenesis. For instance, fibrillar collagens have been shown to provide a co-stimulatory pathway for osteoclastogenesis through interaction with the osteoclast-associated receptor (OSCAR), a regulator of osteoclastogenesis expressed on the surface of pre-osteoclast cells. Based on this rationale, here we design two OSCAR-binding peptides and one recombinant OSCAR-binding protein, and we combine them with peptide-based hydrogels to study their effect on osteoclastogenesis. The OSCAR-binding peptides adopt the collagen triple-helical conformation and interact with OSCAR, as shown by circular dichroism spectropolarimetry and surface plasmon resonance. Furthermore, they have a positive effect on osteoclastogenesis, as demonstrated by appropriate gene expression and tartrate-resistant acid phosphatase staining typical of osteoclast formation. Combination of the OSCAR-binding peptides or the OSCAR-binding recombinant protein with peptide-based hydrogels enhances osteoclast differentiation when compared to the non-modified hydrogels, as demonstrated by multi-nucleation and by F-actin staining showing a characteristic osteoclast-like morphology. We envisage that these hydrogels could be used as a platform to study osteoclastogenesis and, in particular, to investigate the effect of costimulatory pathways involving OSCAR.
Electrocardiographic Changes with Age in Japanese Patients with Noonan Syndrome
Authors: Ichikawa Yasuhiro , Kuroda Hiroyuki , Ikegawa Takeshi , Kawai Shun , Ono Shin , Kim Ki-Sung , Yanagi Sadamitsu , Kurosawa Kenji , Aoki Yoko , Iwamoto Mari , Ueda Hideaki
Published: December 2023

Little information is available on age-related electrocardiographic changes in patients with Noonan syndrome. This single-center study evaluated the electrocardiograms of patients with Noonan syndrome. We divided the patients (n = 112; electrocardiograms, 256) into four groups according to age: G1 (1 month–1 year), G2 (1–6 years), G3 (6–12 years), and G4 (>12 years). Typical Noonan syndrome-related electrocardiographic features such as left-axis deviation, abnormal Q wave, wide QRS complex, and small R wave in precordial leads were detected. A high percentage of QRS axis abnormalities was found in all groups. Significant differences in right-axis deviation (RAD) were noted among the groups: 56.5% of G1 patients showed RAD compared with 33.3% of G2, 21.1% of G3, and 19.2% of G4 patients. The small R was also significantly different among the groups: 32.6% of G1 patients showed a small R wave compared with 14.9% of G2, 8.5% of G3, and 15.4% of G4 patients. Of the 53 patients with Noonan syndrome aged 1 month to 2 years, 18 had T-positive V1 with a higher prevalence of pulmonary stenosis and cardiac interventions. QRS axis abnormalities, small R in V6, and T-positive V1 could help diagnose Noonan syndrome in infants or young children.
Can Concurrent Fibrate Use Reduce Cardiovascular Risks among Moderate Chronic Kidney Disease Patients Undergoing Statin Therapy? A Cohort Study
Authors: Ma Li-Yi , Fan Pei-Chun , Chen Chao-Yu , Tu Yi-Ran , Hsiao Ching-Chung , Yen Chieh-Li , Chang Chih-Hsiang
Published: December 2023

The role of fibrates in treating hypertriglyceridemia in chronic kidney disease (CKD) patients to prevent cardiovascular disease (CVD) has been insufficiently investigated. Since statin is considered the first-line treatment for dyslipidemia in CKD patients, this study aims to evaluate the role of concurrent fibrate therapy with statins among moderate CKD patients. We recruited CKD3 patients from the Chang Gung Research Database who were receiving statin treatment but had not previously been administered ezetimibe or niacin. The participants were divided into two groups based on their use of fibrates (fibrate group) or those with triglyceride levels >200 mg/dL without fibrate treatment (non-fibrate group). The fibrate group (n = 954) only exhibited a significantly lower incidence of AMI (4.4% vs. 5.4%, HR: 0.77, 95% CI: 0.61 to 0.98). The risk of major adverse cardiovascular and cerebrovascular events (14.7% vs. 15.6%, HR: 0.91, 95% CI: 0.72 to 1.15) and all-cause mortality (5.7% vs. 6.1%, HR: 0.91, 95% CI: 0.63 to 1.30) did not significantly differ between the fibrate group and the non-fibrate group (n = 2358). In moderate CKD patients, combining fibrate therapy with statins may not offer additional cardiovascular protection compared to statin alone.
Advances in MRI-Based Assessment of Rectal Cancer Post-Neoadjuvant Therapy: A Comprehensive Review
Authors: Miranda Joao , Causa Andrieu Pamela , Nincevic Josip , Gomes de Farias Lucas de Padua , Khasawneh Hala , Arita Yuki , Stanietzky Nir , Fernandes Maria Clara , De Castria Tiago Biachi , Horvat Natally
Published: December 2023

Rectal cancer presents significant diagnostic and therapeutic challenges, with neoadjuvant therapy playing a pivotal role in improving resectability and patient outcomes. MRI serves as a critical tool in assessing treatment response. However, differentiating viable tumor tissue from therapy-induced changes on MRI remains a complex task. In this comprehensive review, we explore treatment options for rectal cancer based on resectability status, focusing on the role of MRI in guiding therapeutic decisions. We delve into the nuances of MRI-based evaluation of treatment response following neoadjuvant therapy, paying particular attention to emerging techniques like radiomics. Drawing from our insights based on the literature, we provide essential recommendations for post-neoadjuvant therapy management of rectal cancer, all within the context of MRI-based findings.
Contralateral Snare Cannulation vs. Retrograde Gate Cannulation during Endovascular Aortic Repair in Difficult Iliac Artery Anatomy: A Single Center Experience
Authors: Sena Giuseppe , Montemurro Rossella , Pezzo Francesco , Gioffrè Rosario , Gallelli Giuseppe , Rubino Paolo
Published: December 2023

Objective: Endovascular aneurysm repair is well established as the gold standard in treating abdominal aortic aneurysms. Generally, endovascular repair is performed using a bi or trimodular stent graft, requiring placement of a contralateral iliac limb. Deployment of the contralateral iliac limb requires retrograde gate cannulation of the endograft main body contralateral limb. This step represents the crucial point of a standard endovascular repair procedure and can become challenging, especially in the case of high iliac tortuosity. This study compares the procedural times between the retrograde gate cannulation and the contralateral snare cannulation to demonstrate the possibility of directly performing the contralateral snare cannulation in the case of a complex iliac anatomy assessed by the iliac tortuosity index. Methods: One hundred and forty-eight patients with infrarenal abdominal aortic aneurysms who underwent endovascular aneurysm repair from 2017 to 2022 were analyzed retrospectively. Cannulation times between retrograde gate cannulation and contralateral snare cannulation were compared for each degree of iliac tortuosity. The degree of iliac tortuosity was assessed through the iliac tortuosity index. Cannulation times were detected from inserting the wire into the introducer to passing through the radio-opaque gate markers. Results: The cannulation times were 2.94 min for the retrograde gate cannulation group and 3.15 min for the contralateral snare cannulation group, respectively, with no statistically significant differences (p = 0.33). Overall cannulation times were 2.98 min. For the iliac tortuosity index grade 0, the cannulation times were 2.71 min for the retrograde gate cannulation group and 3.85 min for the contralateral snare cannulation group, respectively, with a significant difference in favor of the retrograde gate cannulation group (p < 0.0001). For the iliac tortuosity index grade 1, the cannulation times were 2.74 min for the retrograde gate cannulation group and 2.8 min for the contralateral snare cannulation group, respectively, with no statistically significant differences (p = 0.63). Regarding the iliac tortuosity index grades 2 and 3, the cannulation times were 3.01 and 4.93 min for the retrograde gate cannulation group and 2.71 and 3.28 min for the contralateral snare cannulation group, respectively. The first group’s times were significantly higher than the second group’s (p = 0.01 and p = 0.0001). Conclusions: In patients with infrarenal abdominal aortic aneurysms undergoing endovascular aortic repair, the gate cannulation times were significantly shorter for the contralateral snare cannulation method than the retrograde gate cannulation method in the iliac tortuosity index grades 2 and 3. Therefore, performing the contralateral snare cannulation method would be appropriate.
Minimum Clinically Important Difference (MCID) and Patient Acceptable Symptomatic State (PASS) Applied to the SF-36 in Patients Who Underwent Arthroscopic Rotator Cuff Repair
Authors: Longo Umile Giuseppe , De Salvatore Sergio , Piergentili Ilaria , Lalli Alberto , Bandini Benedetta , Denaro Vincenzo
Published: December 2023

The 36-Item Short-Form Health Survey questionnaire (SF-36) is a reliable tool to assess the health-related quality of life of patients. If a mean difference between pre-operative evaluation and final follow-up is found to be statistically significant, then the change in score is not random. However, a statistically significant mean change may not correspond to a clinical amelioration for the patient or mean that the patient’s state of health is to be considered acceptable. For this reason, interest in the concepts of minimal clinically important difference (MCID) and patient acceptable symptomatic state (PASS) has grown within recent years. The goal of the present work of research was to determine the MCID and PASS values for the SF-36 in patients who received rotator cuff repair (RCR). Forty-six patients (18 women and 28 men, mean age 58.5 ± 12.9) previously diagnosed with rotator cuff disease were enrolled. All of these patients underwent RCR. They were evaluated pre-operatively and six months after the surgical intervention as a final follow-up. The SF-36 questionnaire was assessed at each evaluation. The MCID cut-offs of the total, physical, and mental dimensions of the SF-36 for patients who underwent RCR were 23.1, 32.5, and 18.1, respectively. A 23.1 improvement in the SF-36 score at six months following RCR can be correlated with patients having reached a clinically significant improvement in health status. If 81.9 or more is attained in the SF-36 score after surgical repair, the symptom state can be judged as satisfactory by the majority of patients.