bluuebunny/crossref_metadata_2025 huggingface dataset:
Ageing of Al-Mn-Cu-Be Alloys for Stimulating Precipitation of Icosahedral Quasicrystals
Authors: Bončina Tonica , Albu Mihaela , Zupanič Franc
Published: July 2020

In this work, the ageing of some Al-Mn-Cu-Be alloys was investigated in the temperature range in which predominantly icosahedral quasicrystalline (IQC) precipitates can form. The alloys were cast into a copper mould, directly aged (T5 heat treatment) between 300 and 440 °C for different times. Afterwards, they were examined using scanning and transmission electron microscopy, X-ray diffraction and hardness testing. The main aim of the work was to determine the conditions at which a high number density of spherical icosahedral quasicrystalline precipitates can form. The highest number density of IQC precipitates was obtained at 300 °C after prolonged ageing. The spheroidal precipitates had a diameter less than 20 nm. The size of IQC precipitates increased with the increasing temperature, and in addition, decagonal quasicrystalline precipitates appeared. The time to maximum hardness decreased strongly with increasing ageing temperature. The IQC precipitates can form in a fairly broad temperature range in Al-Mn-Cu-Be alloys and that by varying ageing temperature and duration, rather different distributions of precipitates can be obtained. The presence of precipitates caused rather strong aluminium alloys and fast work hardening during initial plastic deformation.
Fracture–Mechanical Assessment of the Effect of Defects on the Fatigue Lifetime and Limit in Cast and Additively Manufactured Aluminum–Silicon Alloys from HCF to VHCF Regime
Authors: Tenkamp Jochen , Awd Mustafa , Siddique Shafaqat , Starke Peter , Walther Frank
Published: July 2020

Aluminum–silicon alloys are commonly used in die-cast and additively manufactured (AM) light-weight components due to their good processability and high strength-to-weight ratio. As both processing routes lead to the formation of defects such as gas and shrinkage porosity, a defect-sensitive design of components is necessary for safe application. This study deals with the fatigue and crack propagation behavior of die-cast alloy AlSi7Mg0.3 and additively manufactured alloy AlSi12 and its relation to process-induced defects. The different porosities result in significant changes in the fatigue stress-lifetime (S–N) curves. Therefore, the local stress intensity factors of crack-initiating defects were determined in the high and very high cycle fatigue regime according to the fracture mechanics approach of Murakami. Through correlation with fatigue lifetime, the relationship of stress intensity factor (SIF) and fatigue lifetime (N) could be described by one power law (SIF–N curve) for all porosities. The relationship between fatigue limit and defect size was further investigated by Kitagawa–Takahashi (KT) diagrams. By using El Haddad’s intrinsic crack length, reliable differentiation between fracture and run out of the cast and AM aluminum alloys could be realized. SIF–N curves and KT diagrams enable a reliable fatigue design of cast and AM aluminum alloys for a finite and infinite lifetime.
Computing the Growth of Small Cracks in the Assist Round Robin Helicopter Challenge
Authors: Jones Rhys , Peng Daren , Raman R.K. Singh , Huang Pu
Published: July 2020

Sustainment issues associated with military helicopters have drawn attention to the growth of small cracks under a helicopter flight load spectrum. One particular issue is how to simplify (reduce) a measured spectrum to reduce the time and complexity of full-scale helicopter fatigue tests. Given the costs and the time scales associated with performing tests, a means of computationally assessing the effect of a reduced spectrum is desirable. Unfortunately, whilst there have been a number of studies into how to perform a damage tolerant assessment of helicopter structural parts there is currently no equivalent study into how to perform the durability analysis needed to determine the economic life of a helicopter component. To this end, the present paper describes a computational study into small crack growth in AA7075-T7351 under several (reduced) helicopter flight load spectra. This study reveals that the Hartman-Schijve (HS) variant of the NASGRO crack growth equation can reasonably accurately compute the growth of small naturally occurring cracks in AA7075-T7351 under several simplified variants of a measured Black Hawk flight load spectra.
Effect of Thermomechanical Processing on Fatigue Behavior in Solid-State Additive Manufacturing of Al-Mg-Si Alloy
Authors: Rutherford Ben A. , Avery Dustin Z. , Phillips Brandon J. , Rao Harish M. , Doherty Kevin J. , Allison Paul G. , Brewer Luke N. , Jordon J. Brian
Published: July 2020

This work presents, for the first time, an in-depth investigation of the structure–property–fatigue relationships of an Al-Mg-Si alloy (AA6061) processed via additive friction stir-deposition (AFS-D). As industry focus continues to shift for more efficient and lightweight structures, quantitative studies on the cyclic performance of additively manufactured materials are needed. In this study, the AFS-D processed AA6061-T6 was machined into specimens in two orthogonal orientations and subjected to monotonic and strain-controlled fatigue testing. The microstructural features of as-deposited AA6061 exhibited evidence of dynamic recrystallization and grain refinement. In addition, significant reduction in the intermetallic particles was observed after AFS-D processing. The fatigue results demonstrate that the as-deposited material, particularly the longitudinal direction, exhibited similar fatigue performance to wrought AA6061-T6 in both low-cycle and high-cycle fatigue regimes, which is a promising result for additively manufactured material in the as-deposited condition. By contrast, the as-deposited build direction orientation possessed slightly lower fatigue resistance than the wrought feedstock material. The AFS-D material was observed to exhibit different damage mechanisms from porosity-based damage mechanisms observed in fusion-based additively manufactured materials. Lastly, a microstructure-sensitive fatigue model was employed to capture the fatigue effects of the AFS-D processing on the AA6061.
Find the Needle in the Haystack, Then Find It Again: Replication and Validation in the ‘Omics Era
Authors: Perng Wei , Aslibekyan Stella
Published: July 2020

Advancements in high-throughput technologies have made it feasible to study thousands of biological pathways simultaneously for a holistic assessment of health and disease risk via ‘omics platforms. A major challenge in ‘omics research revolves around the reproducibility of findings—a feat that hinges upon balancing false-positive associations with generalizability. Given the foundational role of reproducibility in scientific inference, replication and validation of ‘omics findings are cornerstones of this effort. In this narrative review, we define key terms relevant to replication and validation, present issues surrounding each concept with historical and contemporary examples from genomics (the most well-established and upstream ‘omics), discuss special issues and unique considerations for replication and validation in metabolomics (an emerging field and most downstream ‘omics for which best practices remain yet to be established), and make suggestions for future research leveraging multiple ‘omics datasets.
Lactobacillus reuteri DSM 17938 as a Novel Topical Cosmetic Ingredient: A Proof of Concept Clinical Study in Adults with Atopic Dermatitis
Authors: Butler Éile , Lundqvist Christoffer , Axelsson Jakob
Published: July 2020

Atopic Dermatitis (AD) is a chronically relapsing skin condition characterized by dry, itchy, and inflamed skin where sufferers can frequently be subject to infections. Probiotics are known to be potent immune-modulators, and live Lactobacillus reuteri DSM 17938 has shown to be anti-inflammatory but also to possess antimicrobial and barrier function properties. This study aimed to investigate and compare two investigational ointment products (topical probiotic and control) for cutaneous acceptability, safety, and efficacy under normal conditions of use, in adult subjects with atopic dermatitis. The products were applied twice daily for 8 weeks, and cutaneous acceptability, SCORAD index, local SCORAD, and adverse events were evaluated after 4 and 8 weeks of treatment. At the end of the observations, it was demonstrated that both the probiotic-containing and probiotic-free ointments were both cutaneously acceptable and safe. It importantly showed a statistically and clinically significant improvement of the SCORAD index and local SCORAD in adult subjects with AD after 4 and 8 weeks of continuous use. In conclusion, we show evidence that the probiotic product, containing live L. reuteri DSM 17938 as an extra ingredient, is safe and promising as a novel topical cosmetic ointment and with further testing could be a standard topical product for the management of atopic dermatitis or other disorders associated with the skin.
Bacillus subtilis HU58 and Bacillus coagulans SC208 Probiotics Reduced the Effects of Antibiotic-Induced Gut Microbiome Dysbiosis in an M-SHIME® Model
Authors: Marzorati Massimo , Van den Abbeele Pieter , Bubeck Sarah S. , Bayne Thomas , Krishnan Kiran , Young Aicacia , Mehta Dilip , DeSouza Anselm
Published: July 2020

Benefits associated with probiotic use have been reported; however, the mechanisms behind these benefits are poorly understood. The effects of a probiotic formulation (MegaDuo™) containing Bacillus coagulans SC208 and Bacillus subtilis HU58 on intestinal permeability and immune markers was assessed using a combination of the in vitro gut model, the mucosal simulator of the human intestinal microbial ecosystem (M-SHIME®), and an in vitro inflammatory bowel disease-like Caco-2/THP1 co-culture model in both healthy and antibiotic-induced dysbiosis conditions. Established M-SHIME® proximal colon vessels were treated with/without clindamycin (1 week) and then with/without daily MegaDuo™ treatment (2 weeks). The mucosal and luminal microbial communities were sampled weekly. Suspensions were removed from the proximal colon vessels after 1 and 2 weeks of MegaDuo™ treatment and added to the co-culture system. Transepithelial resistance (membrane barrier function), cytokine/chemokine release, and NFκB activity were then measured. Under conditions of antibiotic-induced dysbiosis, suspensions from MegaDuo™ treated vessels showed reduced gut membrane barrier damage and decreased levels of TNFα and IL-6 compared with suspensions from untreated vessels; no appreciable differences were observed under healthy conditions. MegaDuo™ treatment had no effect on NFκB activity of THP1-Blue™ cells. The potential benefits of MegaDuo™ treatment appeared most evident after 2 weeks of treatment.
Construction of a High-Expression System in Bacillus through Transcriptomic Profiling and Promoter Engineering
Authors: Miao Cui-Cui , Han Lin-Li , Lu Yan-Bing , Feng Hong
Published: July 2020

Bacillus subtilis is an ideal host for secretion and expression of foreign proteins. The promoter is one of the most important elements to facilitate the high-level production of recombinant protein. To expand the repertoire of strong promoters for biotechnological applications in Bacillus species, 14 highly transcribed genes based on transcriptome profiling of B. pumilus BA06 were selected and evaluated for their promoter strength in B. subtilis. Consequently, a strong promoter P2069 was obtained, which could drive the genes encoding alkaline protease (aprE) and green fluorescent protein (GFP) to express more efficiency by an increase of 3.65-fold and 18.40-fold in comparison with the control promoter (PaprE), respectively. Further, promoter engineering was applied to P2069, leading to a mutation promoter (P2069M) that could increase GFP expression by 3.67-fold over the wild-type promoter (P2069). Moreover, the IPTG-inducible expression systems were constructed using the lac operon based on the strong promoters of P2069 and P2069M, which could work well both in B. subtilis and B. pumilus. In this study, highly efficient expression system for Bacillus was constructed based on transcriptome data and promoter engineering, which provide not only a new option for recombinant expression in B. subtilis, but also novel genetic tool for B. pumilus.
Immune Transcriptome of Cells Infected with Enterovirus Strains Obtained from Cases of Type 1 Diabetes
Authors: Poma Anello Marcello , Genoni Angelo , Broccolo Francesco , Denaro Maria , Pugliese Alberto , Basolo Fulvio , Toniolo Antonio
Published: July 2020

Enterovirus (EV) infection of insulin-producing pancreatic beta cells is associated with type 1 diabetes (T1D), but little is known about the mechanisms that lead the virus to cause a persistent infection and, possibly, to induce beta cell autoimmunity. A cell line susceptible to most enterovirus types was infected with EV isolates from cases of T1D and, for comparison, with a replication-competent strain of coxsackievirus B3. The transcription of immune-related genes and secretion of cytokines was evaluated in infected vs. uninfected cells. Acutely infected cells showed the preserved transcription of type I interferon (IFN) pathways and the enhanced transcription/secretion of IL6, IL8, LIF, MCP1, and TGFB1. On the other hand, infection by defective EV strains obtained from diabetic subjects suppressed IFN pathways and the transcription of most cytokines, while enhancing the expression of IL8, IL18, IL32, and MCP1. IL18 and IL32 are known for their pathogenic role in autoimmune diabetes. Thus, the cytokine profile of AV3 cells infected by diabetes-derived EV strains closely matches that observed in patients at the early stages of T1D. The concordance of our results with clinically verified information reinforces the hypothesis that the immune changes observed in type 1 diabetic patients are due to a hardly noticeable virus infection.
Structures of the Skin Microbiome and Mycobiome Depending on Skin Sensitivity
Authors: Keum Hye Lim , Kim Hanbyul , Kim Hye-Jin , Park Taehun , Kim Seoyung , An Susun , Sul Woo Jun
Published: July 2020

Sensitive skin (SS) syndrome is a globally widespread, self-diagnosed discomfort characterized by subjective complaints. Although the skin microbiome is considered important in skin health, the relationship between the skin microbiome and skin sensitivity is still unknown. Here, we aimed to (i) investigate whether the microbiome and mycobiome of SS are distinct from those of non-sensitive skin (NS), and (ii) define the characteristics of the skin microbiome associated with skin sensitivity. A total of 42 Korean women subjects were recruited (SS, n = 23; NS, n = 19) and the microbiome/mycobiome of their right facial cheeks were analyzed. We identified the differential microbiome and mycobiome structures between SS and NS. The mycobiome of SS was more phylogenetically diverse than that of NS. Lactobacillus and Mucor racemosus were more abundant on SS than NS, whereas Malassezia restricta was less abundant. Interestingly, both skin microbiome and mycobiome varied according to the perceived skin sensitivities of the subjects. This study suggests that the skin microbiome and mycobiome are associated with skin sensitivity. Accordingly, it lays the foundation for developing microbiome-based cosmetics or remedies for individuals suffering from SS syndrome.
Current Evidence on the Ocular Surface Microbiota and Related Diseases
Authors: Petrillo Francesco , Pignataro Danilo , Lavano Maria Annunziata , Santella Biagio , Folliero Veronica , Zannella Carla , Astarita Carlo , Gagliano Caterina , Franci Gianluigi , Avitabile Teresio , Galdiero Marilena
Published: July 2020

The ocular surface microbiota refers to the resident non-pathogenic microorganisms that colonize conjunctiva and cornea. Several studies have shown that ocular surface epithelial cells can respond selectively to specific components of ocular pathogenic bacteria by producing pro-inflammatory cytokines and, in contrast, they do not respond to non-pathogenic bacteria, thus supporting the colonization by a real microbiota. However, the analysis of the ocular microbiome composition is essential for understanding the pathophysiology of various ophthalmic diseases. In this scenario, the first studies, which used microbiological culture techniques, reported a less diverse profile of the ocular microbiota compared with that recently discovered using new molecular-based methods. Indeed, until a few years ago, the microbiota of the ocular surface appeared to be dominated by Gram-positive and a few Gram-negative bacteria, as well as some fungal strains. In contrast, genomics has nowadays detected a remarkable diversity in the ocular surface microorganisms. Furthermore, recent studies suggest that the microbiota of other areas of the body, such as the gut and oral microbiota, are involved in the pathophysiology of several ophthalmic diseases. The aim of the present study is to highlight the current evidence on the ocular surface microbiota to better understand it and to investigate its potential role in the development of ophthalmic diseases.
Revealing the Potential of Lipid and β-Glucans Coproduction in Basidiomycetes Yeast
Authors: Byrtusová Dana , Shapaval Volha , Holub Jiří , Šimanský Samuel , Rapta Marek , Szotkowski Martin , Kohler Achim , Márová Ivana
Published: July 2020

Beta (β)–glucans are polysaccharides composed of D-glucose monomers. Nowadays, β-glucans are gaining attention due to their attractive immunomodulatory biological activities, which can be utilized in pharmaceutical or food supplementation industries. Some carotenogenic Basidiomycetes yeasts, previously explored for lipid and carotenoid coproduction, could potentially coproduce a significant amount of β–glucans. In the present study, we screened eleven Basidiomycetes for the coproduction of lipids and β–glucans. We examined the effect of four different C/N ratios and eight different osmolarity conditions on the coproduction of lipids and β–glucans. A high-throughput screening approach employing microcultivation in microtiter plates, Fourier Transform Infrared (FTIR) spectroscopy and reference analysis was utilized in the study. Yeast strains C. infirmominiatum CCY 17-18-4 and R. kratochvilovae CCY 20-2-26 were identified as the best coproducers of lipids and β-glucans. In addition, C. infirmominiatum CCY 17-18-4, R. kratochvilovae CCY 20-2-26 and P. rhodozyma CCY 77-1-1 were identified as the best alternative producers of β-glucans. Increased C/N ratio led to increased biomass, lipid and β-glucans production for several yeast strains. Increased osmolarity had a negative effect on biomass and lipid production while the β-glucan production was positively affected.
Methodical Considerations and Resistance Evaluation against F. graminearum and F. culmorum Head Blight in Wheat. The Influence of Mixture of Isolates on Aggressiveness and Resistance Expression
Authors: Mesterhazy Akos , Gyorgy Andrea , Varga Monika , Toth Beata
Published: July 2020

In resistance tests to Fusarium head blight (FHB), the mixing of inocula before inoculation is normal, but no information about the background of mixing was given. Therefore, four experiments (2013–2015) were made with four independent isolates, their all-possible (11) mixtures and a control. Four cultivars with differing FHB resistance were used. Disease index (DI), Fusarium damaged kernels (FDK) and deoxynivalenol (DON) were evaluated. The isolates used were not stable in aggressiveness. Their mixtures did not also give a stable aggressiveness; it depended on the composition of mix. The three traits diverged in their responses. After the mixing, the aggressiveness was always less than that of the most pathogenic component was. However, in most cases it was significantly higher than the arithmetical mean of the participating isolates. A mixture was not better than a single isolate was. The prediction of the aggressiveness level is problematic even if the aggressiveness of the components was tested. Resistance expression is different in the mixing variants and in the three traits tested. Of them, DON is the most sensitive. More reliable resistance and toxin data can be received when instead of one more independent isolates are used. This is important when highly correct data are needed (genetic research or cultivar registration).
The Significance of Bacillus spp. in Disease Suppression and Growth Promotion of Field and Vegetable Crops
Authors: Miljaković Dragana , Marinković Jelena , Balešević-Tubić Svetlana
Published: July 2020

Bacillus spp. produce a variety of compounds involved in the biocontrol of plant pathogens and promotion of plant growth, which makes them potential candidates for most agricultural and biotechnological applications. Bacilli exhibit antagonistic activity by excreting extracellular metabolites such as antibiotics, cell wall hydrolases, and siderophores. Additionally, Bacillus spp. improve plant response to pathogen attack by triggering induced systemic resistance (ISR). Besides being the most promising biocontrol agents, Bacillus spp. promote plant growth via nitrogen fixation, phosphate solubilization, and phytohormone production. Antagonistic and plant growth-promoting strains of Bacillus spp. might be useful in formulating new preparations. Numerous studies of a wide range of plant species revealed a steady increase in the number of Bacillus spp. identified as potential biocontrol agents and plant growth promoters. Among different mechanisms of action, it remains unclear which individual or combined traits could be used as predictors in the selection of the best strains for crop productivity improvement. Due to numerous factors that influence the successful application of Bacillus spp., it is necessary to understand how different strains function in biological control and plant growth promotion, and distinctly define the factors that contribute to their more efficient use in the field.
C3 Opsonization of Anthrax Bacterium and Peptidoglycan Supports Recognition and Activation of Neutrophils
Authors: Popescu Narcis I. , Keshari Ravi S. , Cochran Jackie , Coggeshall K. Mark , Lupu Florea
Published: July 2020

Neutrophils are the most abundant innate cell population and a key immune player against invading pathogens. Neutrophils can kill both bacterium and spores of Bacillus anthracis, the causative anthrax pathogen. Unlike interactions with professional phagocytes, the molecular recognition of anthrax by neutrophils is largely unknown. In this study, we investigated the role of complement C3 deposition on anthrax particles for neutrophil recognition of bacterium and/or its cell wall peptidoglycan, an abundant pathogen-associated molecular pattern that supports anthrax sepsis. C3 opsonization and recognition by complement receptors accounted for 70–80% of the affinity interactions between neutrophils and anthrax particles at subphysiologic temperatures. In contrast, C3 supported up to 50% of the anthrax particle ingestion under thermophysiologic conditions. Opsonin-dependent low affinity interactions and, to a lower extent, opsonin-independent mechanisms, provide alternative entry routes. Similarly, C3 supported 58% of peptidoglycan-induced degranulation and, to a lower extent, 23% of bacterium-induced degranulation. Interestingly, an opsonin independent mechanism mediated by complement C5, likely through C5a anaphylatoxin, primes azurophilic granules in response to anthrax particles. Overall, we show that C3 deposition supports anthrax recognition by neutrophils but is dispensable for pathogen ingestion and neutrophil degranulation, highlighting immune recognition redundancies that minimize the risk of pathogen evasion.
Metabarcoding Analyses of Gut Microbiota of Nile Tilapia (Oreochromis niloticus) from Lake Awassa and Lake Chamo, Ethiopia
Authors: Bereded Negash Kabtimer , Curto Manuel , Domig Konrad J. , Abebe Getachew Beneberu , Fanta Solomon Workneh , Waidbacher Herwig , Meimberg Harald
Published: July 2020

The Nile tilapia (Oreochromis niloticus) gut harbors a diverse microbial community; however, their variation across gut regions, lumen and mucosa is not fully elucidated. In this study, gut microbiota of all samples across gut regions and sample types (luminal content and mucosa) were analyzed and compared from two Ethiopian lakes. Microbiota were characterized using 16S rRNA Illumina MiSeq platform sequencing. A total of 2061 operational taxonomic units (OTUs) were obtained and the results indicated that Nile tilapia from Lake Chamo harbored a much more diversified gut microbiota than Lake Awassa. In addition, the gut microbiota diversity varied significantly across the gut region based on the Chao1, Shannon and Simpson index. The microbiome analyses of all samples in the midgut region showed significantly higher values for alpha diversity (Chao 1, Shannon and Simpson). Beta diversity analysis revealed a clear separation of samples according to sampling areas and gut regions. The most abundant genera were Clostridium_sensu_stricto and Clostridium_XI genera across all samples. Between the two sampling lakes, two phyla, Phylum Fusobacteria and Cyanobacteria, were found to be significantly different. On the other hand, six phyla (Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Proteobacteria and Cyanobacteria) were significantly different across gut regions. In this study, we found that all samples shared a large core microbiota, comprising a relatively large number of OTUs, which was dominated by Proteobacteria, Firmicutes, Cyanobacteria, Fusobacteria and Actinobacteria. This study has established the bases for future large-scale investigations of gut microbiota of fishes in Ethiopian lakes.
Bioactivity of Serratiochelin A, a Siderophore Isolated from a Co-Culture of Serratia sp. and Shewanella sp.
Authors: Schneider Yannik , Jenssen Marte , Isaksson Johan , Hansen Kine Østnes , Andersen Jeanette Hammer , Hansen Espen H.
Published: July 2020

Siderophores are compounds with high affinity for ferric iron. Bacteria produce these compounds to acquire iron in iron-limiting conditions. Iron is one of the most abundant metals on earth, and its presence is necessary for many vital life processes. Bacteria from the genus Serratia contribute to the iron respiration in their environments, and previously several siderophores have been isolated from this genus. As part of our ongoing search for medicinally relevant compounds produced by marine microbes, a co-culture of a Shewanella sp. isolate and a Serratia sp. isolate, grown in iron-limited conditions, was investigated, and the rare siderophore serratiochelin A (1) was isolated with high yields. Compound 1 has previously been isolated exclusively from Serratia sp., and to our knowledge, there is no bioactivity data available for this siderophore to date. During the isolation process, we observed the degradation product serratiochelin C (2) after exposure to formic acid. Both 1 and 2 were verified by 1-D and 2-D NMR and high-resolution MS/MS. Here, we present the isolation of 1 from an iron-depleted co-culture of Shewanella sp. and Serratia sp., its proposed mechanism of degradation into 2, and the chemical and biological characterization of both compounds. The effects of 1 and 2 on eukaryotic and prokaryotic cells were evaluated, as well as their effect on biofilm formation by Staphylococcus epidermidis. While 2 did not show bioactivity in the given assays, 1 inhibited the growth of the eukaryotic cells and Staphylococcus aureus.
Characterization of the Role of a Non-GPCR Membrane-Bound CFEM Protein in the Pathogenicity and Germination of Botrytis cinerea
Authors: Arya Gulab Chand , Srivastava Dhruv Aditya , Pandaranayaka Eswari P. J. , Manasherova Ekaterina , Prusky Dov Bernard , Elad Yigal , Frenkel Omer , Dvir Hay , Harel Arye
Published: July 2020

The necrotrophic fungus Botrytis cinerea, is considered a major cause of postharvest losses in a wide range of crops. The common fungal extracellular membrane protein (CFEM), containing a conserved eight-cysteine pattern, was found exclusively in fungi. Previous studies in phytopathogenic fungi have demonstrated the role of membrane-bound and secreted CFEM-containing proteins in different aspects of fungal virulence. However, non-G protein-coupled receptor (non-GPCR) membrane CFEM proteins have not been studied yet in phytopathogenic fungi. In the present study, we have identified a non-GPCR membrane-bound CFEM-containing protein, Bcin07g03260, in the B. cinerea genome, and generated deletion mutants, ΔCFEM-Bcin07g03260, to study its potential role in physiology and virulence. Three independent ΔCFEM-Bcin07g03260 mutants showed significantly reduced progression of a necrotic lesion on tomato (Solanum lycopersicum) leaves. Further analysis of the mutants revealed significant reduction (approximately 20–30%) in conidial germination and consequent germ tube elongation compared with the WT. Our data complements a previous study of secreted ΔCFEM1 mutants of B. cinerea that showed reduced progression of necrotic lesions on leaves, without effect on germination. Considering various functions identified for CFEM proteins in fungal virulence, our work illustrates a potential new role for a non-GPCR membrane CFEM in pathogenic fungi to control virulence in the fungus B. cinerea.
Biodiversity of Algae and Cyanobacteria in Biological Soil Crusts Collected Along a Climatic Gradient in Chile Using an Integrative Approach
Authors: Samolov Elena , Baumann Karen , Büdel Burkhard , Jung Patrick , Leinweber Peter , Mikhailyuk Tatiana , Karsten Ulf , Glaser Karin
Published: July 2020

Biocrusts are associations of various prokaryotic and eukaryotic microorganisms in the top millimeters of soil, which can be found in every climate zone on Earth. They stabilize soils and introduce carbon and nitrogen into this compartment. The worldwide occurrence of biocrusts was proven by numerous studies in Europe, Africa, Asia and North America, leaving South America understudied. Using an integrative approach, which combines morphological and molecular characters (small subunit rRNA and ITS region), we examined the diversity of key biocrust photosynthetic organisms at four sites along the latitudinal climate gradient in Chile. The most northern study site was located in the Atacama Desert (arid climate), followed by open shrubland (semiarid climate), a dry forest region (Mediterranean climate) and a mixed broad leaved-coniferous forest (temperate climate) in the south. The lowest species richness was recorded in the desert (18 species), whereas the highest species richness was observed in the Mediterranean zone (40 species). Desert biocrusts were composed exclusively of single-celled Chlorophyta algae, followed by cyanobacteria. Chlorophyta, Streptophyta and cyanobacteria dominated semiarid biocrusts, whereas Mediterranean and temperate Chilean biocrusts were composed mostly of Chlorophyta, Streptophyta and Ochrophyta. Our investigation of Chilean biocrust suggests high biodiversity of South American biocrust phototrophs.
Development of Ceramic Materials for the Manufacture of Bricks with Stone Cutting Sludge from Granite
Authors: Terrones-Saeta Juan María , Suárez-Macías Jorge , Corpas-Iglesias Francisco Antonio , Korobiichuk Valentyn , Shamrai Volodymyr
Published: July 2020

The manufacture of bricks for building purposes consumes large quantities of virgin materials, such as clay. On the other hand, the ornamental stone processing industry produces a huge amount of stone cutting sludge in its process. Therefore, this study presents the development of ceramic materials for the manufacture of bricks with stone cutting sludges, more specifically from granite. For this purpose, the physical properties of the stone cutting sludge and the chemical composition were mainly analyzed. Subsequently, different groups of ceramic samples were conformed and sintered with various combinations of clay and of stone cutting sludges. The conformed samples were evaluated with different physical tests and with the compressive strength test. The addition of stone cutting sludges to the ceramics reflected the creation of a material with lower density and higher porosity. The compressive strength of the different groups reflected a maximum allowable percentage of stone cutting sludges incorporation of 70%. Therefore, ceramic materials were developed with stone cutting sludges, developing a sustainable, lighter material with acceptable mechanical and physical characteristics. Avoiding the deposition of a polluting waste in a landfill and at the same time avoiding the extraction of new virgin materials.
Tellurium Minerals: Structural and Chemical Diversity and Complexity
Authors: Krivovichev Vladimir G. , Krivovichev Sergey V. , Charykova Marina V.
Published: July 2020

The chemical diversity and complexity of tellurium minerals were analyzed using the concept of mineral systems and Shannon informational entropy. The study employed data for 176 Te mineral species known today. Tellurium minerals belong to six mineral systems in the range of one-to-six species-defining elements. For 176 tellurium minerals, only 36 chemical elements act as essential species-defining constituents. The numbers of minerals of main elements are calculated as follows (the number of mineral species is given in parentheses): O (89), H (48), Cu (48), Pb (43), Bi (31), S (29), Ag (20), Fe (20), Pd (16), Cl (13), and Zn (11). In accordance with their chemistry, all Te minerals are classified into five types of mineral systems: tellurium, oxides, tellurides and intermetalides, tellurites, and tellurates. A statistical analysis showed positive relationships between the chemical, structural, and crystallochemical complexities and the number of essential species-defining elements in a mineral. A positive statistically significant relationship between chemical and structural complexities was established. It is shown that oxygen-free and oxygen-bearing Te minerals differ sharply from each other in terms of chemical and structural complexity, with the first group of minerals being simpler than the second group. The oxygen-free Te minerals (tellurium, tellurides, and intermetallides) are formed under reducing conditions with the participation of hydrothermal solutions. The most structurally complex oxygen-bearing Te minerals originate either from chemical weathering and the oxidation of ore deposits or from volcanic exhalations (Nabokoite).
CO2 Hydrogenation over Unsupported Fe-Co Nanoalloy Catalysts
Authors: Calizzi Marco , Mutschler Robin , Patelli Nicola , Migliori Andrea , Zhao Kun , Pasquini Luca , Züttel Andreas
Published: July 2020

The thermo-catalytic synthesis of hydrocarbons from CO2 and H2 is of great interest for the conversion of CO2 into valuable chemicals and fuels. In this work, we aim to contribute to the fundamental understanding of the effect of alloying on the reaction yield and selectivity to a specific product. For this purpose, Fe-Co alloy nanoparticles (nanoalloys) with 30, 50 and 76 wt% Co content are synthesized via the Inert Gas Condensation method. The nanoalloys show a uniform composition and a size distribution between 10 and 25 nm, determined by means of X-ray diffraction and electron microscopy. The catalytic activity for CO2 hydrogenation is investigated in a plug flow reactor coupled with a mass spectrometer, carrying out the reaction as a function of temperature (393–823 K) at ambient pressure. The Fe-Co nanoalloys prove to be more active and more selective to CO than elemental Fe and Co nanoparticles prepared by the same method. Furthermore, the Fe-Co nanoalloys catalyze the formation of C2-C5 hydrocarbon products, while Co and Fe nanoparticles yield only CH4 and CO, respectively. We explain this synergistic effect by the simultaneous variation in CO2 binding energy and decomposition barrier as the Fe/Co ratio in the nanoalloy changes. With increasing Fe content, increased activation temperatures for the formation of CH4 (from 440 K to 560 K) and C2-C5 hydrocarbons (from 460 K to 560 K) are observed.
Magnetic Mg-Fe/LDH Intercalated Activated Carbon Composites for Nitrate and Phosphate Removal from Wastewater: Insight into Behavior and Mechanisms
Authors: Alagha Omar , Manzar Mohammad Saood , Zubair Mukarram , Anil Ismail , Mu’azu Nuhu Dalhat , Qureshi Aleem
Published: July 2020

This experimental work focused on the synthesis, characterization, and testing of a unique, magnetically separable, and eco-friendly adsorbent composite material for the advanced treatment and efficient removal of nitrate and phosphate pollutants from wastewater. The MgAl-augmented double-layered hydroxide (Mg-Fe/LDH) intercalated with sludge-based activated carbon (SBAC-MgFe) composites were characterized by FT-IR, XRD, BET, VSM, SEM, and TEM techniques, revealing homogeneous and efficient dispersion of MgFe/LDH within the activated carbon (AC) matrix, a highly mesoporous structure, and superparamagnetic characteristics. The initial solution pH, adsorbent dose, contact time, and temperature parameters were optimized in order to reach the best removal performance for both pollutants. The maximum adsorption capacities of phosphate and nitrate were found to be 110 and 54.5 mg/g, respectively. The competition between phosphate and coexisting ions (Cl−, CO32−, and SO42−) was studied and found to be remarkably lower in comparison with the nitrate adsorption. The adsorption mechanisms were elucidated by kinetic, isotherm, thermodynamic modeling, and post-adsorption characterizations of the composite. Modeling and mechanistic studies demonstrated that physisorption processes such as electrostatic attraction and ion exchange mainly governed the nitrate and phosphate adsorption. The composite indicated an outstanding regeneration performance even after five sequences of adsorption/desorption cycles. The fabricated composite with magnetically separable characteristics can be used as a promising adsorbent for the removal of phosphate and nitrate pollutants from wastewater.
3D Networks of Ge Quantum Wires in Amorphous Alumina Matrix
Authors: Basioli Lovro , Tkalčević Marija , Bogdanović-Radović Iva , Dražić Goran , Nadazdy Peter , Siffalovic Peter , Salamon Krešimir , Mičetić Maja
Published: July 2020

Recently demonstrated 3D networks of Ge quantum wires in an alumina matrix, produced by a simple magnetron sputtering deposition enables the realization of nanodevices with tailored conductivity and opto-electrical properties. Their growth and ordering mechanisms as well as possibilities in the design of their structure have not been explored yet. Here, we investigate a broad range of deposition conditions leading to the formation of such quantum wire networks. The resulting structures show an extraordinary tenability of the networks’ geometrical properties. These properties are easily controllable by deposition temperature and Ge concentration. The network’s geometry is shown to retain the same basic structure, adjusting its parameters according to Ge concentration in the material. In addition, the networks’ growth and ordering mechanisms are explained. Furthermore, optical measurements demonstrate that the presented networks show strong confinement effects controllable by their geometrical parameters. Interestingly, energy shift is the largest for the longest quantum wires, and quantum wire length is the main parameter for control of confinement. Presented results demonstrate a method to produce unique materials with designable properties by a simple self-assembled growth method and reveal a self-assembling growth mechanism of novel 3D ordered Ge nanostructures with highly designable optical properties.
Dielectrophoretic Crossover Frequency of Single Particles: Quantifying the Effect of Surface Functional Groups and Electrohydrodynamic Flow Drag Force
Authors: Lu Yun-Wei , Sun Chieh , Kao Ying-Chuan , Hung Chia-Ling , Juang Jia-Yang
Published: July 2020

We present a comprehensive comparison of dielectrophoretic (DEP) crossover frequency of single particles determined by various experimental methods and theoretical models under the same conditions, and ensure that discrepancy due to uncertain or inconsistent material properties and electrode design can be minimized. Our experiment shows that sulfate- and carboxyl-functionalized particles have higher crossover frequencies than non-functionalized ones, which is attributed to the electric double layer (EDL). To better understand the formation of the EDL, we performed simulations to study the relationship between initial surface charge density, surface ion adsorption, effective surface conductance, and functional groups of both functionalized and nonfunctionalized particles in media with various conductivities. We also conducted detailed simulations to quantify how much error may be introduced if concurrent electrohydrodynamic forces, such as electrothermal and electro-osmotic forces, are not properly avoided during the crossover frequency measurement.
Effects of Different TiO2 Nanoparticles Concentrations on the Physical and Antibacterial Activities of Chitosan-Based Coating Film
Authors: Xing Yage , Li Xuanlin , Guo Xunlian , Li Wenxiu , Chen Jianwen , Liu Qian , Xu Qinglian , Wang Qin , Yang Hua , Shui Yuru , Bi Xiufang
Published: July 2020

In this investigation, the effect of different concentrations of titanium dioxide (TiO2) nanoparticles (NPs) on the structure and antimicrobial activity of chitosan-based coating films was examined. Analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) revealed that the modified TiO2 NPs were successfully dispersed into the chitosan matrix, and that the roughness of the chitosan-TiO2 nanocomposites were significantly reduced. Moreover, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analyses indicated that the chitosan interacted with TiO2 NPs and possessed good compatibility, while a thermogravimetric analysis (TGA) of the thermal properties showed that the chitosan-TiO2 nanocomposites with 0.05% TiO2 NPs concentration had the best thermal stability. The chitosan-TiO2 nanocomposite exhibited an inhibitory effect on the growth of Escherichia coli and Staphylococcus aureus. This antimicrobial activity of the chitosan-TiO2 nanocomposites had an inhibition zone ranging from 9.86 ± 0.90 to 13.55 ± 0.35 (mm). These results, therefore, indicate that chitosan-based coating films incorporated with TiO2 NPs might become a potential packaging system for prolonging the shelf-life of fruits and vegetables.
Differential Pulse Voltammetric Electrochemical Sensor for the Detection of Etidronic Acid in Pharmaceutical Samples by Using rGO-Ag@SiO2/Au PCB
Authors: Panneer Selvam Sathish , Chinnadayyala Somasekhar R. , Cho Sungbo , Yun Kyusik
Published: July 2020

An rGO-Ag@SiO2 nanocomposite-based electrochemical sensor was developed to detect etidronic acid (EA) using the differential pulse voltammetric (DPV) technique. Rapid self-assembly of the rGO-Ag@SiO2 nanocomposite was accomplished through probe sonication. The developed rGO-Ag@SiO2 nanocomposite was used as an electrochemical sensing platform by drop-casting on a gold (Au) printed circuit board (PCB). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) confirmed the enhanced electrochemical active surface area (ECASA) and low charge transfer resistance (Rct) of the rGO-Ag@SiO2/Au PCB. The accelerated electron transfer and the high number of active sites on the rGO-Ag@SiO2/Au PCB resulted in the electrochemical detection of EA through the DPV technique with a limit of detection (LOD) of 0.68 μM and a linear range of 2.0–200.0 μM. The constructed DPV sensor exhibited high selectivity toward EA, high reproducibility in terms of different Au PCBs, excellent repeatability, and long-term stability in storage at room temperature (25 °C). The real-time application of the rGO-Ag@SiO2/Au PCB for EA detection was investigated using EA-based pharmaceutical samples. Recovery percentages between 96.2% and 102.9% were obtained. The developed DPV sensor based on an rGO-Ag@SiO2/Au PCB could be used to detect other electrochemically active species following optimization under certain conditions.
Oxygen Pressure Influence on Properties of Nanocrystalline LiNbO3 Films Grown by Laser Ablation
Authors: Vakulov Zakhar , Zamburg Evgeny , Khakhulin Daniil , Geldash Andrey , Golosov Dmitriy A. , Zavadski Sergey M. , Miakonkikh Andrey V. , Rudenko Konstantin V. , Dostanko Anatoliy P. , He Zhubing , Ageev Oleg A.
Published: July 2020

Energy conversion devices draw much attention due to their effective usage of energy and resulting decrease in CO2 emissions, which slows down the global warming processes. Fabrication of energy conversion devices based on ferroelectric and piezoelectric lead-free films is complicated due to the difficulties associated with insufficient elaboration of growth methods. Most ferroelectric and piezoelectric materials (LiNbO3, BaTiO3, etc.) are multi-component oxides, which significantly complicates their integration with micro- and nanoelectronic technology. This paper reports the effect of the oxygen pressure on the properties of nanocrystalline lithium niobate (LiNbO3) films grown by pulsed laser deposition on SiO2/Si structures. We theoretically investigated the mechanisms of LiNbO3 dissociation at various oxygen pressures. The results of x-ray photoelectron spectroscopy study have shown that conditions for the formation of LiNbO3 films are created only at an oxygen pressure of 1 × 10−2 Torr. At low residual pressure (1 × 10−5 Torr), a lack of oxygen in the formed films leads to the formation of niobium oxide (Nb2O5) clusters. The presented theoretical and experimental results provide an enhanced understanding of the nanocrystalline LiNbO3 films growth with target parameters using pulsed laser deposition for the implementation of piezoelectric and photoelectric energy converters.
Improved Thermophysical Properties and Energy Efficiency of Aqueous Ionic Liquid/MXene Nanofluid in a Hybrid PV/T Solar System
Authors: Das Likhan , Habib Khairul , Saidur R. , Aslfattahi Navid , Yahya Syed Mohd , Rubbi Fazlay
Published: July 2020

In recent years, solar energy technologies have developed an emerging edge. The incessant research to develop a power source alternative to fossil fuel because of its scarcity and detrimental effects on the environment is the main driving force. In addition, nanofluids have gained immense interest as superior heat transfer fluid in solar technologies for the last decades. In this research, a binary solution of ionic liquid (IL) + water based ionanofluids is formulated successfully with two dimensional MXene (Ti3C2) nano additives at three distinct concentrations of 0.05, 0.10, and 0.20 wt % and the optimum concentration is used to check the performance of a hybrid solar PV/T system. The layered structure of MXene and high absorbance of prepared nanofluids have been perceived by SEM and UV–vis respectively. Rheometer and DSC are used to assess the viscosity and heat capacity respectively while transient hot wire technique is engaged for thermal conductivity measurement. A maximum improvement of 47% in thermal conductivity is observed for 0.20 wt % loading of MXene. Furthermore, the viscosity is found to rise insignificantly with addition of Ti3C2 by different concentrations. Conversely, viscosity decreases substantially as the temperature increases from 20 °C to 60 °C. However, based on their thermophysical properties, 0.20 wt % is found to be the optimum concentration. A comparative analysis in terms of heat transfer performance with three different nanofluids in PV/T system shows that, IL+ water/MXene ionanofluid exhibits highest thermal, electrical, and overall heat transfer efficiency compared to water/alumina, palm oil/MXene, and water alone. Maximum electrical efficiency and thermal efficiency are recorded as 13.95% and 81.15% respectively using IL + water/MXene, besides that, heat transfer coefficients are also noticed to increase by 12.6% and 2% when compared to water/alumina and palm oil/MXene respectively. In conclusion, it can be demonstrated that MXene dispersed ionanofluid might be great a prospect in the field of heat transfer applications since they can augment the heat transfer rate considerably which improves system efficiency.