bluuebunny/crossref_metadata_2025 huggingface dataset:
Cannabidiol Modulates Neuroinflammatory Markers in a PTSD Model Conducted on Female Rats
Authors: Portugalov Anna , Peled Gaia , Zorin Sharon , Akirav Irit
Published: October 2024

Post-traumatic stress disorder (PTSD) is a debilitating neuropsychiatric condition closely linked to neuroinflammation, with a higher prevalence in women. Cannabidiol (CBD), a non-psychoactive cannabinoid, has shown promise as a potential treatment for PTSD. In this study, we used a PTSD model in which female rats were subjected to a severe foot shock followed by contextual situational reminders (SRs). Testing was conducted one month after exposure. The rats received daily CBD injections for three weeks during the SRs, from days 7 to 28. Two days after the final SR, the rats underwent five extinction trials, followed by the forced swim test (FST). After a five-day rest period, the rats were sacrificed, and brain tissues from the medial prefrontal cortex (mPFC) and ventral subiculum (vSUB) were analyzed for inflammatory markers. Chronic CBD treatment reversed impairments in fear extinction caused by shock and SR. It also reduced learned helplessness in the FST and decreased the upregulation of mPFC-il1β induced by shock and SRs. Additionally, exposure to shock and SRs downregulated mPFC-il6 while upregulating vSUB-il6. CBD treatment further downregulated il6 expression in the vSUB compared to the vehicle groups. Our findings show that CBD effectively inhibited the development of PTSD-like behaviors and suppressed neuroinflammation in the mPFC.
“A Friend Among Strangers” or the Ambiguous Roles of Runx2
Authors: Azarkina Kseniia , Gromova Ekaterina , Malashicheva Anna
Published: October 2024

The transcription factor Runx2 plays a crucial role in regulating osteogenic differentiation and skeletal development. This factor not only controls the expression of genes involved in bone formation, but also interacts with signaling pathways such as the Notch pathway, which are essential for body development. However, studies have produced conflicting results regarding the relationship between Runx2 and the Notch pathway. Some studies suggest a synergistic interaction between these molecules, while others suggest an inhibitory one, for example, the interplay between Notch signaling, Runx2, and vitamin D3 in osteogenic differentiation and bone remodeling. The findings suggest a complex relationship between Notch signaling and osteogenic differentiation, with ongoing research needed to clarify the mechanisms involved and resolve existing contradictions regarding role of Notch in this process. Additionally, there is increasing evidence of contradictory roles for Runx2 in various tissues and organs, both under normal conditions and in pathological states. This diversity of roles makes Runx2 a potential therapeutic target, offering new directions for research. In this review, we have discussed the mechanisms of osteogenic differentiation and the important role of Runx2 in this process. We have also examined its relationship with different signaling pathways. However, there are still many uncertainties and inconsistencies in our current understanding of these interactions. Additionally, given that Runx2 is also involved in numerous other events in various tissues, we have tried to comprehensively examine its functions outside the skeletal system.
The Progression of Symptoms in Post COVID-19 Patients: A Multicentre, Prospective, Observational Cohort Study
Authors: Cornelissen Merel E. B. , Haarman Myrthe M. , Twisk Jos W. R. , Houweling Laura , Baalbaki Nadia , Sondermeijer Brigitte , Beijers Rosanne J. H. C. G. , Gach Debbie , Bloemsma Lizan D. , Maitland-van der Zee Anke H.
Published: October 2024

Background: Although the coronavirus disease 2019 (COVID-19) pandemic is no longer a public health emergency of international concern, 30% of COVID-19 patients still have long-term complaints. A better understanding of the progression of symptoms after COVID-19 is needed to reduce the burden of the post COVID-19 condition. Objective: This study aims to investigate the progression of symptoms, identify patterns of symptom progression, and assess their associations with patient characteristics. Methods: Within the P4O2 COVID-19 study, patients aged 40–65 years were recruited from five Dutch hospitals. At 3–6 and 12–18 months post COVID-19, medical data were collected, and pulmonary function tests were performed. In between, symptoms were assessed monthly with a questionnaire. Latent class mixed modelling was used to identify symptom progression patterns over time, with multinomial logistic regression to examine associations with patient characteristics. Results: Eighty-eight patients (aged 54.4 years, 48.9% males) were included. Three trajectories were identified for fatigue and dyspnoea: decreasing, high persistent, and low persistent. The odds of “decreasing fatigue” was higher for never smokers and participants in the lifestyle intervention and lower for those having a comorbidity. The odds of “decreasing dyspnoea” was higher for moderate COVID-19 patients and lifestyle intervention participants and lower for males, mild COVID-19 patients, and those with a higher age. Conclusions: Three distinct trajectories were identified for fatigue and dyspnoea, delineating patterns of symptom persistence following COVID-19. Sex, age, smoking status, participation in lifestyle interventions and COVID-19 severity were associated with the likelihood of belonging to different trajectories. These findings highlight the heterogeneity of the long-term symptoms experienced by post COVID-19 patients and emphasise the importance of personalised treatment strategies.
Comparative Blood Profiling Based on ATR-FTIR Spectroscopy and Chemometrics for Differential Diagnosis of Patients with Amyotrophic Lateral Sclerosis—Pilot Study
Authors: Tkachenko Kateryna , González-Saíz José M. , Calvo Ana C. , Lunetta Christian , Osta Rosario , Pizarro Consuelo
Published: October 2024

Amyotrophic lateral sclerosis (ALS) is a motor neurodegenerative disease characterized by poor prognosis. Currently, screening and diagnostic methods for ALS remain challenging, often leading to diagnosis at an advanced stage of the disease. This delay hinders the timely initiation of therapy, negatively impacting patient well-being. Additionally, misdiagnosis with other neurodegenerative disorders that present similar profiles often occurs. Therefore, there is an urgent need for a cost-effective, rapid, and user-friendly tool capable of predicting ALS onset. In this pilot study, we demonstrate that infrared spectroscopy, coupled with chemometric analysis, can effectively identify and predict disease profiles from blood samples drawn from ALS patients. The selected predictive spectral markers, which are used in various discriminant models, achieved an AUROC sensitivity of almost 80% for distinguishing ALS patients from controls. Furthermore, the differentiation of ALS at both the initial and advanced stages from other neurodegenerative disorders showed even higher AUROC values, with sensitivities of 87% (AUROC: 0.70–0.97). These findings highlight the elevated potential of ATR-FTIR spectroscopy for routine clinical screening and early diagnosis of ALS.
Perfectionism and Adolescent Athletes’ Burnout: The Serial Mediation of Motivation and Coping Style
Authors: Xu Aiai , Luo Xiaobing , Qiu Xueqing , Lu Changfen
Published: October 2024

Preventing and reducing adolescent athletes’ risk of burnout can help promote long-term sports participation, improve performance, and maintain psychological well-being. The present study examined the associations between perfectionism and burnout among Chinese adolescent athletes and the mediating role of motivation and coping styles. A total of 243 Chinese adolescent athletes (78% boys; Mage = 17.8; SD = 2.62) completed the Sport Multidimensional Perfectionism Scale for China, the Situational Motivation Scale, the Coping Scale for Chinese Athletes, and the Athlete Burnout Questionnaire to assess perfectionism (strivings and concerns), coping styles (problem-focused and emotion-focused), motivation (intrinsic and amotivation), and burnout. Path analyses indicated that intrinsic motivation and coping styles (problem-focused and emotion-focused) serially mediated the relationship between perfectionistic strivings and burnout. Problem-focused coping mediated the relationship between perfectionistic concerns and burnout. These findings contribute to a model of the effect of perfectionism on adolescent athletes’ burnout, provide support for the self-determination theory, and suggest a feasible approach for mitigating burnout in this group.
Lightweight Strategies for Wooden-Structure Buildings Based on Embodied Carbon Emission Calculations for Carbon Reduction
Authors: Zhai Yukun , Li Yunan , Tang Su , Liu Yixuan , Liu Yazhuo
Published: October 2024

To achieve carbon reduction in architecture, this study establishes a carbon emission calculation model for wooden structures based on life cycle assessment (LCA) theory, using the emission factor method. Carbon emission factors involved in the entire life cycle of wooden buildings are identified and calculated for two modern wooden structures at Beijing Forestry University. The results are quantified and compared to analyze the causes of high carbon emissions, and lightweight design strategies for wooden structures are proposed through case studies. The two case buildings consumed 0.36 m3 and 0.29 m3 of wood material per square meter of building area, with carbon emissions of 311.23 kgCO2e/m2 and 292.03 kgCO2e/m2, respectively. During the building life cycle, waste disposal, material production, and material transportation accounted for the highest carbon emissions, accounting for 40%, 25%, and 20%, respectively. This study shows that factors such as the building shape coefficient, structural design, component design, material type, and decoration influence material usage in wooden structures, thereby affecting carbon emissions. Key strategies for reducing embodied carbon include optimizing building shape and structural design, using lightweight materials, and minimizing decoration.
Experimental Study on Mechanical Properties and Stability of Marine Dredged Mud with Improvement by Waste Steel Slag
Authors: Ji Qiaoling , Wang Yingjian , Jia Xiuru , Cheng Yu
Published: October 2024

As marine-dredged mud and waste steel slag in coastal port cities continue to soar, the traditional treatment method of land stockpiling has caused ecological problems. Thus, it is necessary to find a large-scale resource-comprehensive utilization method for dredged mud and waste steel slag. This study uses waste steel slag and composite solidifying agents (cement, lime, fly ash) to physically and chemically improve marine-dredged mud. The physical improvement effect of the particle size and dosage of waste steel slag was studied by the shear strength test under the effect of freeze–thaw cycle. Then, based on the Box–Behnken design of the response surface method, the interaction effects of the solidifying agent components on the unconfined compressive strength were studied. Then, the water stability under dry–wet cycles and a microscopic mechanism were analyzed by XRD and SEM tests. The results show that the waste steel slag with a dosage of 30% and a particle size of 1.18~2.36 mm has the best improvement. The interaction between cement and lime and lime and fly ash has a significant effect on the linear effect and surface effect of 7d unconfined compressive strength, and the strength increases first and then decreases with the increase in its dosage. For the 14d unconfined compressive strength, only the interaction between cement and lime is still significant. The unconfined compressive strength prediction model is established to optimize the mix ratio of the composite solidifying agent. In the water stability, the water stability coefficients of the 7d and 14d tests are 0.68 and 0.95, respectively, and the volume and mass loss rates are all below 1.5%, showing a good performance in dry–wet resistance and durability. Microscopic mechanism analysis shows that waste steel slag provides an ‘anchoring surface’ as a skeleton, which improves the pore structure of dredged mud, and the hydration products generated by the solidifying agent play a role in filling and cementation. The results of the study can provide an experimental and technical basis for the resource engineering of marine-dredged mud and waste steel slag, helping the construction of green low-carbon and resource-saving ports.
Seismic Responses and Overturning Resistance Capacity of Base-Isolated Structures Under the Influence of Pounding Interactions with Adjacent Structures
Authors: Si Shengzhe , Zhang Jingcai , Zhang Chunwei
Published: October 2024

Seismic accelerations and interlayer displacements can be reduced by Laminated Rubber Bearings (LRBs) efficiently. Isolators would amplify the displacement of the superstructure by extending the natural period, thereby reducing acceleration and seismic damage. However, as a result, the risk of pounding with adjacent structures would be raised. This study investigated the seismic responses and overturning resistance capacity of base-isolated structures subjected to pounding against an adjacent structure. Parameter studies were conducted to evaluate the effects of gap size, pounding stiffness, and horizontal stiffness of the isolation layer. Results show that poundings are characterized by intense, short forces causing acceleration spikes, amplifying the overturning coefficient and risk. The overturning risk initially decreases then increases with gap size under pulse-like earthquakes, while wider gaps mitigate effects during non-pulse events. Increased pounding stiffness intensifies poundings, heightening vulnerability. The structure’s overturning resistance initially improves with increased horizontal stiffness of the isolation layer but declines excessively with further stiffness increase.
Novel Application of a Swift-Assembled Support Method with Prefabricated Corrugated Steel for Vertical Shaft Excavation in a Metro Station
Authors: Wang Xingkuo , Zhang Maohui , Wu Shiqian , Lin Yanjun , Song Peng , Fan Wei , Wei Huanwei , Zheng Xiao
Published: October 2024

For the construction of a subway station, temporary vertical shafts were commonly used to facilitate machine operation. In densely urban areas, the requirement of settlement control and environmental impact called for a novel construction method of vertical shafts. In this paper, a novel swift-assembled support (SAS) structure and construction method for vertical shafts of a metro station was proposed, using prefabricated steel components. A comprehensive scheme of full-time monitoring was conducted to evaluate the performance of this novel support structure and ground response. Field monitoring results indicated that the SAS method was able to control the settlement of ground and adjacent buildings. Based on the field measurements, the calculation theory for design parameters were discussed. The active earth pressure yield from the method considering wall movement was closer to the field measurements. All of the local buckling values were both overestimated based on the technical standards’ methods. The calculation methods were thereby adopted carefully to determine the designed loading share ratio of structure components. The advantage of the SAS method, including rapid construction, safety, and lower environmental impacts, were obviously clear.
Comparative Efficacy of Adagrasib and Sotorasib in KRAS G12C-Mutant NSCLC: Insights from Pivotal Trials
Authors: Peng Tzu-Rong , Wu Ta-Wei , Yi Tai-Yung , Wu An-Jan
Published: October 2024

Background: The KRAS G12C mutation, prevalent in various malignancies, including non-small cell lung cancer (NSCLC), represents a unique therapeutic target. Adagrasib and sotorasib, two FDA-approved agents specifically targeting this mutation, have shown promise in clinical trials. This study aims to compare their efficacy in treating KRAS G12C-mutated NSCLC, drawing insights from pivotal clinical trials. Methods: We analyzed data from three key clinical trials: KRYSTAL-1, CodeBreak100, and CodeBreak200. Our methodology involved reconstructing individual patient data from published Kaplan–Meier curves using the IPDfromKM tool (Version 0.1.10). The primary endpoints were progression-free survival (PFS) and overall survival (OS), evaluated through hazard ratios (HRs) and the restricted mean survival time (RMST) method. Results: The HR for PFS favored adagrasib (HR: 0.90 [95% CI: 0.69, 1.19], p = 0.473), suggesting a non-significant trend toward better disease control compared to sotorasib. For OS, the HR was 0.99 [95% CI: 0.75, 1.33] (p = 0.969), indicating no significant difference between the two drugs. RMST analysis supported these findings, with adagrasib showing a consistently higher RMST in PFS at 6, 12, and 18 months. However, OS benefits converged over time, with adagrasib marginally surpassing sotorasib by the 18-month mark. Conclusions: This comprehensive analysis reveals that while adagrasib may offer a slight advantage in PFS, both drugs demonstrate comparable efficacy in OS for KRAS G12C-mutated NSCLC. The subtle differences observed, particularly in PFS, could inform clinical decision-making, emphasizing the need for personalized treatment strategies. Future research should focus on long-term effects and identifying patient subgroups that may benefit more from one drug over the other.
Utility and Safety of 5-ALA Guided Surgery in Pediatric Brain Tumors: A Systematic Review
Authors: Wang Cheng , Yu Ying , Wang Yafei , Yu Jiahua , Zhang Chenran
Published: October 2024

Background: 5-Aminolevulinic acid-guided surgery for adult gliomas has been approved by the European Medicines Agency and the US Food and Drug Administration, becoming a reliable tool for improving gross total resection rates and patient outcomes. This has led several medical centers to explore the off-label use of 5-ALA in the resection of pediatric brain tumors, assessing its efficacy and safety across various tumor types. However, given the differences between children and adults, the appropriateness of 5-ALA use in pediatric populations has not yet been fully established. Methods: We collected eligible publications from Embase, Scopus, PubMed, and Proquest, ultimately selecting 27 studies. Data extraction and retrospective analysis of 249 surgical cases were conducted to determine the current efficacy and safety of 5-ALA in pediatric brain tumors. The fluorescence rate and utility stratified by several clinical features, including WHO grade, tumor classification, and tumor location, were analyzed. Results: Most studies suggest that 5-ALA can enhance tumor identification in high-grade tumors, including glioblastomas and anaplastic astrocytomas. Changes in survival or recurrence rates associated with 5-ALA-guided resection have not been reported. None of the cases reported significant postoperative complications related to the use of 5-ALA. Conclusions: 5-ALA can aid in the resection of high-grade gliomas in pediatric patients. The efficacy of 5-ALA in low-grade gliomas and other tumors may require enhancement with additional tools or modified administration protocols. The safety of 5-ALA has reached a preliminary consensus, although further randomized controlled trials and data on survival and molecular characteristics are needed.
Cutaneous Toxicities of Advanced Treatment for Cutaneous Melanoma: A Prospective Study from a Single-Center Institution
Authors: Venturi Federico , Veronesi Giulia , Scotti Biagio , Dika Emi
Published: October 2024

Background/Objectives: The landscape of advanced melanoma treatments has shifted dramatically in recent years. Target therapy and immunotherapy have changed the management of patients with both metastatic (stage IV according to AJCC 8th ed.) and nodal (stage IIB/C and III) disease. As the use of novel agents has increased, so have the cutaneous toxicities associated with these medications. While most skin reactions are low-grade and can be managed conservatively with topical therapies, high-grade or life-threatening drug reactions can arise during therapy, requiring prompt dermatologic recognition and treatment. Given the survival benefit attributed to these new agents, treating skin toxicity and maintaining a patient’s quality of life is of paramount importance. Methods: We undertook a prospective, monocentric, and descriptive study in Bologna, Italy, including patients referred to the Oncologic Dermatology Unit of IRCCS AOU of Bologna who developed biopsy-proven cutaneous adverse events (AE) under treatment with immunotherapy for cutaneous melanoma with nodal (stage IIB/C, III) and metastatic (stage IV) disease from January 2016 to April 2024. Results: In 202 identified patients, 75 (37.5%) developed skin AEs. Ipilimumab was causal for 48.1% of skin AEs, followed by nivolumab (37%) and pembrolizumab (31.4%). Recorded types of skin AEs included erythematous rash, vitiligo, alopecia, lichenoid, maculopapular, acneiform, urticarial, psoriasiform, granulomatous, eczematous, and severe cutaneous AEs, such as Erythema multiforme/Stevens-Johnson syndrome and bullous autoimmune dermatoses. Most AEs were low-grade [CTCAE 1–2] (97%) and typically occurred after 10 weeks of treatment. Conclusions: This study comprehensively describes skin AEs occurring during systemic treatment with ICIs for cutaneous melanoma at a single center.
The Role of CT and MR Imaging in Stereotactic Body Radiotherapy of the Spine: From Patient Selection and Treatment Planning to Post-Treatment Monitoring
Authors: Azadbakht Javid , Condos Amy , Haynor David , Gibbs Wende , Jabehdar Maralani Pejman , Sahgal Arjun , Chao Samuel , Foote Matthew , Suh John , Chang Eric , Guckenberger Matthias , Mossa-Basha Mahmud , Lo Simon
Published: October 2024

Spine metastases (SMs) are common, arising in 70% of the cases of the most prevalent malignancies in males (prostate cancer) and females (breast cancer). Stereotactic body radiotherapy, or SBRT, has been incorporated into clinical treatment algorithms over the past decade. SBRT has shown promising rates of local control for oligometastatic spinal lesions with low radiation dose to adjacent critical tissues, particularly the spinal cord. Imaging is critically important in SBRT planning, guidance, and response monitoring. This paper reviews the roles of imaging in spine SBRT, including conventional and advanced imaging approaches for SM detection, treatment planning, and post-SBRT follow-up.
Impact of Antibiotic Stewardship on Treatment of Hospitalized Children with Skin and Soft-Tissue Infections
Authors: Brigadoi Giulia , Rossin Sara , Chiusaroli Lorenzo , Demarin Giulia Camilla , Maestri Linda , Tesser Francesca , Matarazzo Martina , Liberati Cecilia , Barbieri Elisa , Giaquinto Carlo , Da Dalt Liviana , Bressan Silvia , Donà Daniele
Published: October 2024

Background: Skin and soft-tissue infections (SSTIs) are common infectious syndromes in children. Overusing broad-spectrum antibiotics has contributed to rising antibiotic resistance, complicating treatment outcomes. To address this issue, antimicrobial stewardship programs (ASPs) have been implemented to optimize antibiotic use. This study evaluated the impact of a multifaceted ASP on antibiotic prescribing practices for SSTIs in a pediatric acute care setting over eight years. Methods: We conducted a quasi-experimental study at the Pediatric Acute Care Unit of the Padua University Hospital, including children admitted with SSTIs from October 2014 to September 2022, to evaluate the impact of a multifaceted ASP implemented in October 2015. The study was divided into three periods: pre-implementation (October 2014–September 2015), post-implementation (October 2015–March 2020), and COVID-19 (April 2020–August 2022). Data on antibiotic prescriptions and microbiological results were collected and analyzed. Results: The implementation of the ASP led to a significant reduction in the use of broad-spectrum antibiotics, particularly third-generation cephalosporins (from 40.4% to 9.8%) and glycopeptides (from 21.1% to 1.6%). There was a notable increase in the prescription of Access antibiotics, from 30% in the pre-implementation to over 60% in the post-implementation and 80% during COVID-19. No increase in the hospital length of stay was observed. Microbiological results showed no significant changes in bacterial profiles over time. Conclusions: The use of the ASP effectively improved antibiotic prescribing practices, reducing reliance on broad-spectrum antibiotics even during the COVID-19 pandemic. These findings highlight the value of ongoing stewardship efforts and suggest the need for similar programs in ambulatory settings to further address antibiotic resistance.
Prevalence, Risk Factors and Antibiotic Resistance of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli in Children Hospitalized with Urinary Tract Infection at King Abdulaziz University Hospital, Jeddah, Saudi Arabia
Authors: Abdelgalil Abobakr , Saeedi Fajr , Metwalli Eilaf , Almutairi Futoon , Felemban Mayar , Albaradei Hadeel , Aseeri Haneen , Mokhtar Jawahir , Baw Wesam , Sayed Mohamed
Published: October 2024

Background/Objectives: We aimed to assess the prevalence and risk factors for acquisition of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) in children admitted with urinary tract infection (UTI) at a tertiary university hospital in Saudi Arabia, as well as to investigate antibiotic resistance patterns. Methods: This retrospective cross-sectional study involved hospitalized children aged 0–14 years from January 2018 to December 2022 with urine cultures that grew E. coli or ESBL-producing E. coli. Data of the antimicrobial susceptibility for isolated bacteria were collected. Results: This study analyzed 242 urine samples obtained from 119 children with E. coli UTIs. Of these, 20.7% (n = 50) were ESBL producers. Previous antibiotic use (last 3 months), prophylactic antibiotic use, prior UTI (last 3 months), recurrent UTIs, and underlying co-morbidities (p = 0.011, <0.001, 0.025, <0.001, and 0.013, respectively) had a significant relationship with increased risk of ESBL E. coli UTIs. Generally, the highest resistance rates in the ESBL-producing isolates were for ampicillin and third-generation cephalosporin. Conversely, all ESBL-positive isolates were sensitive to meropenem, with variable resistance rates to other antibiotics as amikacin, nitrofurantoin, quinolones and trimethoprim/sulfamethoxazole (2%, 8%, 56% and 64%, respectively). Conclusions: There is a high prevalence of ESBL production among children hospitalized with E. coli UTIs. Addressing ESBL UTI risk factors helps to recognize high-risk cases and enhance proper antibiotic use.
Evaluating Educational Patterns and Methods in Infant Sleep Care: Trends, Effectiveness, and Impact in Home Settings—A Systematic Review
Authors: Aggelou Maria , Metallinou Dimitra , Dagla Maria , Vivilaki Victoria , Sarantaki Antigoni
Published: October 2024

Background: Sleep care is crucial for the health and development of infants, with proper sleep patterns reducing the risk of sudden infant death syndrome (SIDS) and other sleep-related incidents. Educational interventions targeting caregivers are essential in promoting safe sleep practices. Methods: This systematic review adhered to PRISMA guidelines, searching databases such as PubMed, MEDLINE, Scopus, and the Cochrane Library. Inclusion criteria focused on studies involving home-based interventions for infants aged 0–12 months, including parental education and behavioral interventions. Exclusion criteria included studies in clinical settings and non-peer-reviewed articles. Data extraction and synthesis were performed by two independent reviewers, using a narrative approach to categorize interventions and outcomes. Results: Twenty-three studies met the inclusion criteria. Key findings indicate that home-based educational interventions, including hospital-based programs, home visits, and mobile health technologies, significantly improve parental knowledge and adherence to safe sleep practices. These interventions also enhance parental satisfaction and contribute positively to infant health outcomes. Conclusions: Educational interventions have demonstrated effectiveness in promoting safe sleep practices among caregivers, particularly in home settings. These interventions, including hospital-based programs, home visits, and digital tools, improve parental knowledge, adherence to guidelines, and overall satisfaction. The impact is evident in the reduction of unsafe sleep behaviors and enhanced infant health outcomes. However, variability in the intervention methods and delivery, cultural contexts, and geographic focus suggest a need for more tailored, long-term, and comprehensive studies. Future research should standardize outcome measures and assess the sustained impact of these educational strategies on infant sleep patterns and caregiver practices over time. This will provide deeper insights into the trends and long-term effectiveness of educational patterns and methods in diverse home environments.
Glacial Lake Outburst Flood Susceptibility Mapping in Sikkim: A Comparison of AHP and Fuzzy AHP Models
Authors: Das Arindam , Singh Suraj Kumar , Kanga Shruti , Sajan Bhartendu , Meraj Gowhar , Kumar Pankaj
Published: October 2024

The Sikkim region of the Eastern Himalayas is highly susceptible to Glacial Lake Outburst Floods (GLOFs), a risk that has increased significantly due to rapid glacial retreat driven by climate change in recent years. This study presents a comprehensive evaluation of GLOF susceptibility in Sikkim, employing Analytic Hierarchy Process (AHP) and Fuzzy Analytic Hierarchy Process (FAHP) models. Key factors influencing GLOF vulnerability, including lake volume, seismic activity, precipitation, slope, and proximity to rivers, were quantified to develop AHP and FAHP based susceptibility maps. These maps were validated using Receiver Operating Characteristic (ROC) curves, with the AHP method achieving an Area Under the Curve (AUC) of 0.92 and the FAHP method scoring 0.88, indicating high predictive accuracy for both models. A comparison of the two approaches revealed distinct characteristics, with FAHP providing more granular insights into moderate-risk zones, while AHP offered stronger predictive capability for high-risk areas. Our results indicated that the expansion of glacial lakes, particularly over the past three decades, has heightened the potential for GLOFs, highlighting the urgent need for continuous monitoring and adaptive risk mitigation strategies in the region. This study, in addition to enhancing our understanding of GLOF risks in Sikkim, also provides a robust framework for assessing and managing these risks in other glacial regions worldwide.
Analysis of Influence of Ultrasonic Shot Peening on Surface Plastic Behavior of Superalloy
Authors: Shi Xihui , Cai Jin , Zhang Liwen , Pan Yuliang , Wu Hao
Published: October 2024

This work focuses on the effects of ultrasonic shot peening (USP) on grain refinement and orientation behavior in the surface region of GH4151 superalloy. The microstructure evolution of the alloy under USP durations were studied. The effects of USP-induced grain refinement, orientation, and dislocation motion behavior were analyzed. The results indicated that during the USP process, the plastic deformation of the surface layer of superalloys is accompanied by changes in grain size and orientation. The random impact of the spheres on the surface area promotes grain refinement and grain rotation, enhancing the randomness of grain orientations and reducing the texture strength and the proportion of “soft” orientation distribution. Over a long period of treatment, a large number of spheres cause the slip planes and slip directions of each grain to rotate due to the additional shear stress from the impact, resulting in relatively consistent plastic deformation on the surface and the enhanced randomness of grain orientations, thus reducing the high texture strength introduced by previous machining processes. The understanding of dislocation pile-up behavior and the relationship between externally applied shear stress, pile-up characteristics, and grain refinement is essential for optimizing the USP process and achieving the desired material properties.
Amantadine-Heparin-Polypyrrole as a Promising Drug Delivery Reservoir with a Biological Approach
Authors: Kulik Sara , Golba Sylwia , Matuła Izabela , Stodolak-Zych Ewa , Kurpanik Roksana
Published: October 2024

There is an urgent need to alleviate the symptoms of neurodegenerative diseases. The presented work includes the use of electrochemical polymerization (CV) to obtain active polypyrrole layers with incorporated molecules of a neurological drug substance—amantadine hydrochloride. The obtained films were characterized chemically, structurally, and functionally in terms of their use as a drug delivery systems which are neurologically active. FTIR spectra were recorded to identify the incorporation of drug substances into the matrix. The obtained results showed that amantadine and heparin were embedded to the polypyrrole matrix. Scanning electron microscopy (SEM) was used to examine the morphology of the films. The films deposited on the steel substrate showed a compact, smooth structure, where there was no visible organized structure. After release, the film became corrugated. Adhesive tests were conducted with the cross-cut Test Method B to determine the mechanical properties, and the results showed that amantadine improves adhesion for steel substrates. The films were potentially stimulated by chronoamperometry, and UV-Vis spectra were registered to calculate the concentration of AMA in the solution after release. The release curves indicate a 95% efficiency of AMA release over the studied time period and protocol. Later, antibacterial properties were tested. The proposed system was able to provide a daily dose of drugs that ensures a therapeutic effect. This is a significant step towards developing systems capable of delivering a wider range of doses, potentially in line with the full spectrum recommended for therapeutic efficacy. The antibacterial properties of the material allows it to be considered as a material with antibacterial potential in the presence of the Staphylococcus aureus (S. aureus) strain. The percentage reduction ratio indicates a 90–100% reduction of bacteria in the suspension.
Electric Stimulation at 448 kHz Modulates Proliferation and Differentiation of Follicle Dermal Papilla Cells
Authors: Martínez-Pascual María Antonia , Sacristán Silvia , Toledano-Macías Elena , Hernández-Bule María Luisa
Published: October 2024

Dermal papilla cells (DPCs) regulate the hair cycle and play important roles in hair growth and regeneration. Alopecia is a pathology caused by a deregulation in the hair cycle phases. Currently, the use of physical therapies such as radiofrequency (RF) as an alternative to pharmacological treatment is increasing. Electrical stimulation by capacitive resistive electrical transfer (CRET) is one of these therapies. The objective of the present study was to analyze the effect of RF-CRET currents on DPCs. Cells were treated with subthermal 448 kHz CRET currents with two different types of signals: standard (CRET-STD) or modulated (CRET-MOD). Viability (XTT Assay), proliferation (Ki67 and ERK1/2), apoptosis (p53 and caspase 3), differentiation (β-catenin and α-SMA), and anagen markers (versican and PPARγ) were analyzed by immunofluorescence and immunoblot. CRET caused effects on the proliferation and survival of DPCs associated with increases in the expression of p-MAPK-ERK1/2, cyclin D1, and decreases in the expression of p53 and caspase 3. Also, CRET caused significant transient increases in the expression of β-catenin, involved in hair growth, and in the expression of anagen phase markers such as versican and PPARγ related to hair follicle maintenance. The present study highlights the ability of treatment with CRET therapy to cause molecular alterations in DPC involved in hair regeneration.
Perspectives of Regenerative Medicine in Dermatology and Cosmetology
Authors: Berardesca Enzo , Cameli Norma
Published: October 2024

Regenerative medicine has revolutionized the field of biological sciences, pharmacology and healthcare, bringing a new approach to treatments of various health conditions. Dermatology and aesthetic medicine are interested in these new scientific approaches as well. From this point of view, many areas of skin health and cosmetology may be involved, but several questions should be answered before these procedures become routine in offices and hospitals. Indeed scientific, ethical, and financial issues still have to be addressed in detail. Nevertheless, ongoing research and technological advancements are expected to further improve therapies. The integration of artificial intelligence and machine learning could enhance the precision of regenerative treatments, making them more effective and accessible in forthcoming years.
Quantification of Replacement Fibrosis in Aortic Stenosis: A Narrative Review on the Utility of Cardiovascular Magnetic Resonance Imaging
Authors: Rajah Megan R. , Doubell Anton F. , Herbst Philip G.
Published: October 2024

Aortic stenosis (AS) is associated with the development of replacement myocardial fibrosis/scar. Given the dose-dependent relationship between scar and clinical outcomes after aortic valve replacement (AVR) surgery, scar quantity may serve as an important risk-stratification tool to aid decision-making on the optimal timing of AVR. Scar is non-invasively assessed and quantified by cardiovascular magnetic resonance (CMR) imaging. Several quantification techniques exist, and consensus on the optimal technique is lacking. These techniques range from a visual manual method to fully automated ones. This review describes the different scar quantification techniques used and highlights their strengths and shortfalls within the context of AS. The two most commonly used techniques in AS include the semi-automated signal threshold versus reference mean (STRM) and full-width half-maximum (FWHM) techniques. The accuracy and reproducibility of these techniques may be hindered in AS by the coexistence of diffuse interstitial fibrosis and the presence of relatively small, non-bright scars. The validation of these techniques against histology, which is the current gold standard for scar quantification in AS, is limited. Based on the best current evidence, the STRM method using a threshold of three standard deviations above the mean signal intensity of remote myocardium is recommended. The high reproducibility of the FWHM technique in non-AS cohorts has been shown and merits further evaluation within the context of AS. Future directions include the use of quantitative T1 mapping for the detection and quantification of scar, as well as the development of serum biomarkers that reflect the fibrotic status of the myocardium in AS.
Role of Apparent Diffusion Coefficient Value and Apparent Diffusion Coefficient Ratio as Prognostic Factors for Prostate Cancer Aggressiveness
Authors: Buss Arvids , Radzina Maija , Liepa Mara , Birkenfelds Edgars , Saule Laura , Miculis Karlis , Mikelsone Madara , Vjaters Egils
Published: October 2024

Background: Prostate cancer is one of the most prevalent cancers in the male population. To determine the aggressiveness of suspected lesions precisely, predictive models are increasingly being developed using quantitative MRI measurements, and particularly the ADC value. This study aimed to determine whether ADC values could be used to establish the aggressiveness of prostate cancer. Methods: A retrospective single-center study included 398 patients with prostate cancer who underwent a multiparametric MRI prior to radical prostatectomy. DWI ADC values were measured (µm2/s) using b values of 50 and 1000. The dominant lesion best visualized on MRI was analyzed. The ADC values of the index lesion and reference tissue were compared to tumor aggressivity according to the Gleason grade groups based on radical prostatectomy results. Statistical analysis was performed using the Mann–Whitney U test, Kruskal–Wallis H test, Spearman’s rank correlation, and ROC curves. Results: A very strong negative correlation (rs = −0.846, p < 0.001) between ADC and GS was found. ROC analysis revealed an AUC of 0.958 and an ADC threshold value of 758 µm2/s in clinically significant prostate cancer diagnoses using the absolute ADC value, with no advantage of using the ADC ratio over the absolute ADC value being identified. Conclusion: DWI ADC values and the calculated ADC ratio have a significant inverse correlation with GS. The findings indicate a strong capability in determining prostate cancer aggressiveness, as well as the possibility of assisting with assigning PI-RADS categories using ADC as quantitative metrics.
Are Extracorporeal Shock Waves Just a Therapeutic Tool?
Authors: Gialanella Bernardo , Comini Laura , Bonometti Gian Pietro , Vanoglio Fabio , Bettinsoli Andrea , Santoro Raffaele , Olivares Adriana , Luisa Alberto
Published: October 2024

Background: Focused extracorporeal shock wave therapy (ESWT) has been successfully used to treat musculoskeletal conditions, but ESWT stimulates nociceptors, causing pain deep in the tissue during treatment. The occurrence of pain during ESWT is a side effect, but it can help identify painful sites and assess minimum (MiTI) and maximum (MaTI) pain thresholds to ESWT pressure stimuli. This topic has received limited attention in literature. Methods: This observational study describes a specific approach to using ESWT to study pain in 71 patients. The approach proposes moving the ESWT transducer head of the device over the entire joint surface, progressively increasing the energy level until the patient experiences pain. Results: In the study, MiTI and MaTI were 0.218 ± 0.090 and 0.416 ± 0.165 mJ/mm2 in the affected joint and 0.282 ± 0.128 and 0.501 ± 0.174 mJ/mm2 in the contralateral homologous healthy joint, being significantly lower in the affected joint (MiTI: p < 0.001 and MaTI: p = 0.003, respectively). ESWT induced pain in 94.37% of the sites with the highest subjective pain and in a greater number of sites (204) than digital pressure (123) (p < 0.001). All sites with digital pressure pain also had ESWT pain. Conclusions: These results suggest that the ESWT device may be useful in investigating pain in musculoskeletal conditions and tailoring therapy.
The Effect of Advanced Platelet-Rich Fibrin Plus (A-PRF+) on Graft Stability in Dental Implants and Alveolar Ridge Augmentation Procedures: A New Low-Speed Standardized Centrifugation Protocol
Authors: Walch Benjamin , Kolk Andreas , Scheibl Dominik , Guarda Maria , Maier Sarah Christine , Denk Lena
Published: October 2024

Background: Platelet-rich fibrin (PRF) is a concentrate derived from autologous blood, containing platelets, fibrin, and growth factors (GF) obtained through centrifugation. PRF can be mixed with bone replacement material to form sticky bone, which is then introduced into the desired area for stabilizing and graft-covering. Depending on the centrifugation protocol, the effectiveness of the end products can vary. This controlled clinical study examines the impact of our established PRF protocol in alveolar augmentation and dental implant placement on vertical bone loss. Materials and Methods: A total of 362 implants were performed in 170 patients at the Department of Oral and Maxillofacial Surgery at the University Hospital Innsbruck between 2018 and 2021. After accounting for lost implants, we retrospectively evaluated a radiological vertical bone loss exceeding 1 mm in the first year as the primary endpoint. Results: The use of PRF was significantly associated with vertical bone loss > 1 mm (OR = 0.32, 95% CI (0.13–0.81), p = 0.016). There were no significant associations between PRF and the number of implants lost, the type of augmentation, or bone loss. Discussion: A-PRF+ sticky bone grafts, when combined with bone graft materials, show reduced resorption rates, indicating their potential to enhance graft stability in oral implantology. Our data indicate that the Medifuge MF 100 (Silfradent srl, Forlì, Italy) is effective in producing A-PRF+.
Periodontitis Provokes Retinal Neurodegenerative Effects of Metabolic Syndrome: A Cross-Sectional Study
Authors: Arslan Hatice , Yorgancilar Nur , Kose Oguz , Aslan Mehmet Gokhan , Altin Ahmet , Bayrakdar Sevda Kurt , Yemenoglu Hatice , Findik Huseyin , Yilmaz Adnan
Published: October 2024

Background: This cross-sectional study aims to investigate the retino-choroidal degenerative effects of periodontitis, metabolic syndrome (Mets), and the combination of these diseases using optical coherence tomography (OCT) measurements. Methods: Ninety-two patients selected according to inclusion criteria were divided into four groups: systemically and periodontally healthy (control), systemically healthy periodontitis (PD), periodontally healthy metabolic syndrome (MetS), and periodontitis and metabolic syndrome combined (PD-MetS). The systemic inflammatory–oxidative effects of periodontitis and MetS were biochemically evaluated using the serum TNF-α level, IL-1β/IL-10 ratio, and oxidative stress index (OSI: TOS/TAS). Retinal (AMT, pRNFLT, and GCL + T) and choroidal (SFCT) morphometric measurements and vascular evaluations (foveal capillary density) were performed via OCT Angio with swept-source technology. Results: Both periodontitis and Mets cause systemic inflammatory stress characterized by significant increases in the IL-1β/IL-10 ratio and OSI (p < 0.05). Compared to the control group, the AMT was significantly thinner in the MetS group, the pRNFLT was significantly thinner in the PD-MetS group, and the SFCT was significantly thinner in both groups (p < 0.05). The GCL+ was slightly thicker in the Mets groups. (p > 0.05) Foveal capillary density did not differ significantly among the groups. (p > 0.05). Conclusions: Periodontitis-related inflammatory stress alone causes changes in retinal and subfoveal choroidal thicknesses that are not statistically significant. On the other hand, when combined with Mets, it may significantly provoke the retinal neurodegenerative effects of this disease.
Accuracy of One-Piece vs. Segmented Three-Dimensional Printed Transfer Trays for Indirect Bracket Placement
Authors: Alyammahi Bayan , Khamis Amar Hassan , Ghoneima Ahmed
Published: October 2024

Objective: To assess the accuracy of three-dimensional (3D) printed one-piece vs. multiple segmented transfer trays for indirect bonding techniques in moderate and severe crowding cases. Methods: Eighty digital maxillary dental models were produced by an extraoral scanner. 3D-printed one-piece and segmented trays were virtually designed utilizing Maestro 3D Ortho Studio® v4 and printed using a NextDent printer. The sample was classified into two groups: Group 1 (moderate crowding) included 40 digital models with a space deficiency of 6–7 mm, and Group 2 (severe crowding) included 40 digital models with a space deficiency of 10 mm. Ortho classic brackets were then placed into the 3D printed models with the aid of the transfer trays, and the models with the final bracket positioning were scanned using iTero scanner. Four measurements were selected on each tooth to perform the analysis. Mann–Whitney and Kruskal–Wallis tests were used for comparisons. A p-value of ≤ 0.05 was considered statistically significant. Results: In the moderate crowding group, statistically significant differences were detected between the one-piece, segmented, and control groups for three measurements (p < 0.001), while the rest of the measurements showed no significant differences (p > 0.05). In the severe crowding group, no significant differences were detected for any of the measurements. Conclusions: One-piece and segmented 3D-printed transfer trays are considered accurate tools for indirect bonding in moderate and severe malocclusion cases. The severity of crowding did not affect the accuracy of bracket transfer in indirect bonding.