bluuebunny/crossref_metadata_2025 huggingface dataset:
COVID-19 knowledge, attitude, and preventive practices among government and private bank workers in Ethiopia
Authors: Hassen Seada , Adane Metadel
Published: October 2023

Background

Bank workers are among the many service-sector employees who are at risk of COVID-19 infection. Individual’s adherence to control measures is affected by their COVID-19 knowledge, attitudes, and practices (KAP). Since KAP is an important cognitive key in public health prevention and promotion, this study aimed to identify COVID-19 KAP-related gaps among bank workers in Dessie City, Ethiopia and to guide banks and health authorities in taking corrective actions.

Methods

An institution-based cross-sectional study was conducted from January 1st to 30th, 2021 among 413 bank workers. A binary logistic regression was applied to determine association of independent variables with outcome variables using three different models. Variables at 95% confidence interval (CI) with a p < 0.25 from bivariate analysis were transported to three different multivariable logistic regression models and then variables with a p-value of 0.05 from the multivariable analysis of each model were declared as significantly associated with the outcome variables.

Results

The results of this study show that 84.7% [95% CI: 81.1–88.1] of bank workers had good knowledge, 50.4% positive attitude, and 50.6% [95% CI: 45.8–55.0] good practice towards prevention of COVID-19. The only variable significantly associated with knowledge in this study was positive attitude (AOR = 8.89; 95%CI: 3.34–23.64). Being ≥35 years old (AOR = 2.46; 95%CI: 1.25–4.84) and getting information on COVID-19 (AOR = 3.81; 95%CI: 1.84–7.91) were among factors significantly associated with attitude towards COVID-19 prevention, whereas being female and ≥ 35 years old (AOR = 2.56; 95%CI: 1.29–5.06) and (AOR = 2.73; 95% CI: 1.15–6.51), respectively were factors associated with good preventive practice towards COVID-19.

Conclusion and recommendation

Considering those significant factors responsible for determining COVID-19 KAP level of respondents, health education program and information dissemination are recommended, including appropriate strategies by policy makers and bank managers to develop effective interventions for COVID-19 transmission in banks.
Perceptions of Chinese hospital leaders on joint commission international accreditation: a qualitative study
Authors: Zhang HongFan , Huang Siou-Tang , Bittle Mark J. , Engineer Lilly , Chiu Herng-Chia
Published: October 2023

Background

Joint Commission International (JCI) accreditation plays a significant role in improving the quality of care and patient safety worldwide. Hospital leadership is critical in making international accreditation happen with successful implementation. Little is known about how Chinese hospital leaders experienced and perceived the impact of JCI accreditation. This paper is the first study to explore the perceptions of hospital leaders toward JCI accreditation in China.

Methods

Qualitative semi-structured interviews were used to explore the perceptions of the chief operating officers, the chief medical officers, and the chief quality officers in five JCI-accredited hospitals in China. Thematic analysis was used to analyze the interview transcripts and identify the main themes.

Results

Fifteen hospital leaders participated in the interviews. Three themes emerged from the analysis, namely the motivations, challenges, and benefits related to pursuing and implementing JCI accreditation. The qualitative study found that eight factors influenced hospital leadership to pursue JCI accreditation, five challenges were identified with implementing JCI standards, and eight benefits emerged from the leadership perspective.

Conclusion

Pursuing JCI accreditation is a discretionary decision by the hospital leadership. Participants were motivated by prevalent perceptions that JCI requirements would be used as a management tool to improve the quality of care and patient safety in their hospitals. These same organizational leaders identified challenges associated with implementing and sustaining JCI accreditation. The significant challenges were a clear understanding of the foreign accreditation standards, making staff actively participate in JCI processes, and changing staff behaviors accordingly. The top 5 perceived benefits to JCI accreditation from the leaders’ perspective were improved leadership and hospital safety, improvements in the care processes, and the quality of care and the learning culture improved. Other perceived benefits include enhanced reputation, better cost containment, and a sense of pride in the staff in JCI-accredited hospitals.
Is the happiness of Chinese truly the highest in the world? The impact of basic public services on happiness
Authors: Mu Na , Li Shaoting , Wang Zhengbing
Published: October 2023

Based on the survey report by the United Nations Sustainable Development Solutions Network (SDSN) and Ipsos Group, the world ranking of Chinese people’s happiness shows a significant gap. This study attempts to analyze the subjective well-being of Chinese residents through public database from the China Household Finance Survey Center in 2017. An ordered Probit model is constructed to investigate the impact of non-monetary factors, specifically basic public services, on the subjective well-being of Chinese people. The results indicate that: (1) The subjective well-being of Chinese residents is found to be lower than what the survey report indicated. (2) Basic public services have a significant positive impact on residents’ happiness. (3) Social trust played a moderating role, positively influencing the relationship between basic public services and residents’ happiness. (4) The impact of basic public services on happiness varied significantly depending on factors such as age, registered residence, and places of residence. To enhance the happiness of Chinese residents, it is recommended to focus on improving the equalization of basic public services and establishing a robust basic public service system. These measures can effectively contribute to the overall well-being and happiness of the population.
Sex differences and athletic performance. Where do trans individuals fit into sports and athletics based on current research?
Authors: Oberlin D. J.
Published: October 2023

There are well known sex differences in parameters of physical fitness/performance due to changes occurring during sexual development. Thus, many sport and athletic events have regulations separating male and female participants. However, the inclusion or exclusion of transgender individuals in athletics has recently received outsized attention despite relatively few cases of transgender athletes. When determining which athletic gender category trans individuals should be permitted to compete in, it is important to understand the level of physical fitness/performance these individuals possess relative to their cisgender counterparts. Unfortunately, there are few studies investigating this topic, and several complications that confound this research. The current review seeks to discuss sex and gender as concepts, review sex differences in fitness/performance and how they develop, and then, consider how current evidence suggests that trans individuals compare to cis individuals. Finally, this review seeks to offer considerations for whether trans individuals should be excluded from sports and athletics, and how future research should proceed to better understand this marginalized population.
Inactivation of Avian Influenza Virus Aerosol Using Membrane-Less Electrolyzed Water Spraying
Authors: Yang Shinhao , Chuang Chi-Yu , Huang Hsiao-Chien , Fang Wei
Published: October 2023

Avian influenza virus (AIV) can have a serious impact on both human and animal health. In this study, we used an environmentally controlled chamber and a hemagglutination assay (HA) to evaluate the ability of membrane-less electrolyzed water (MLEW) spraying to inactivate H6N1 AIV aerosol. MLEW was generated by electrolyzing sodium chloride solution, and then sprayed into the chamber at free available chlorine (FAC) concentrations of 50, 100 and 200 mg/L by means of high-pressure air pumping to inactivate airborne H6N1 AIV aerosols individually. The results showed that MLEW spraying effectively neutralized H6N1 AIV aerosol. In addition, the virucidal ability of MLEW increased as the FAC concentration increased. Five minutes after MLEW spraying at an FAC concentration of 200 mg/L, the H6N1 viral HA titer decreased from 24 to 20. Our work provides important new evidence of the value of spraying with MLEW disinfectant to protect against AIV, which may be further applied for indoor decontamination purposes to promote animal and human health.
Electrical Distribution System LCA for Future Regional Aircraft—Preliminary Definition of Methodology
Authors: Izquierdo Daniel , Casas Gabriel , Garriga Ana G. , Castro Ignacio , Zieminska-Stolarska Aleksandra , Sobulska Mariia , Zbicinski Ireneusz
Published: October 2023

The Hybrid Electric Regional Aircraft Distribution Technologies (HECATE) Clean Aviation project will mature and develop breakthrough technologies and perform scalability and impact analysis to ensure safe and power-dense technologies that will enable Entry Into Service (EIS) of hybrid-electric regional aircraft by 2035. Along the project, a circular economy approach in future aircraft will be ensured through the use of Life Cycle Assessment (LCA), performing this type of assessment on the overall electrical system and primary/secondary distribution and conversion technologies, helping to be in line with long-term environmental roadmaps such as Flightpath 2050. This communication includes a description of the HECATE activities and how LCA will be applied to the future Regional Aircraft Electrical Distribution System.
A Low-Cost Relative Positioning Method for UAV/UGV Coordinated Heterogeneous System Based on Visual-Lidar Fusion
Authors: Luo Haojun , Wen Chih-Yung
Published: October 2023

Unmanned Ground Vehicles (UGVs) and Unmanned Aerial Vehicles (UAVs) are commonly used for various purposes, and their cooperative systems have been developed to enhance their capabilities. However, tracking and interacting with dynamic UAVs poses several challenges, including limitations of traditional radar and visual systems, and the need for the real-time monitoring of UAV positions. To address these challenges, a low-cost method that uses LiDAR (Light Detection and Ranging) and RGB-D cameras to detect and track UAVs in real time has been proposed. This method relies on a learning model and a linear Kalman filter, and has demonstrated satisfactory estimation accuracy using only CPU (Central Processing Unit)- in GPS (Global Positioning System)-denied environments without any prior information.
Experimental Study on Optimum Design of Aircraft Icing Detection Based on Large-Scale Icing Wind Tunnel
Authors: Ding Liang , Yi Xian , Hu Zhanwei , Guo Xiangdong
Published: October 2023

Icing detection is the premise and basis for the operation of aircraft icing protection system, and is the primary issue in flight safety assurance. At present, there is a lack of research methods and design reference for the layout optimization of ice detectors. Therefore, in order to simulate the real icing environment encountered by the aircraft more accurately, a large-scale icing wind tunnel was used to carry out experimental research on the icing characteristics of the sensor probes. A closed-loop experimental method including the typical condition selection, sensor array interference examination and ice shape repeatability verification was initially proposed. A stepwise optimization process and a sensitivity analysis on ambient conditions were combined to determine the optimal distribution for sensor installation. It is found that the water collection coefficient on the cylinder surface of the probe first increases and then decreases along the axial direction, reaching the extreme value at a certain position. The height of this extreme point will gradually increase with the development of the wall boundary layer, showing a variation range of 2~30 mm. Improper design may cause the sensor probe to fail to capture the point with the maximum icing thickness, affecting the sensitivity of icing detection. In addition, each probe position has different sensitivity to changes in flow parameters; the points with larger icing mass and lower sensitivity to changes in attack angle will have better detection effect. The measured data and analysis in the present work can provide a basis for the accurate design of icing sensor probes.
Application of a Performance-Improvement Method for Small-Size Axial Flow Turbines
Authors: Nicoara Razvan , Crunteanu Daniel , Vilag Valeriu
Published: October 2023

As a main component of most gas-turbine engines, the axial flow turbines have been in a process of continuous improvement, reaching high efficiencies and reliability. A well-known drawback of these systems is the rapid decrease in performance when operating at lower than nominal conditions. Thus, a novel performance-enhancement method for axial turbines operating at partial loads has been previously proposed and numerically characterized. In this paper, one applies the aforementioned method for a smaller size axial flow turbine, part of a gas-generator assembly for a microjet engine, to determine, by the use of CFD analysis, the influence of the system at different partial regimes across the working line. A logical scheme based on iterative steps and multiple numerical simulations is also used to determine the engine response to the injection of compressor bleed air into the turbine passages. The results show, as determined in the previous study, that the generated power can be increased for all partial regimes, with the influence being more noticeable at higher regimes, leading to a reduction in fuel consumption in order to achieve the same regimes.
Dynamic Analysis of Neuron Models
Authors: Wang Yiqiao , Ding Guanghong , Yao Wei
Published: October 2023

Based on the Hodgkin–Huxley theory, this paper establishes several nonlinear system models, analyzes the models’ stability, and studies the conditions for repetitive discharge of neuronal membrane potential. Our dynamic analysis showed that the main channel currents (the fast transient sodium current, the potassium delayed rectifier current, and the fixed leak current) of a neuron determine its dynamic properties and that the GHK formula will greatly widen the stimulation current range of the repetitive discharge condition compared with the Nernst equation. The model including the change in ion concentration will lead to spreading depression (SD)-like depolarization, and the inclusion of a Na-K pump will weaken the current stimulation effect by decreasing the extracellular K accumulation. The results indicate that the Hodgkin–Huxley model is suitable for describing the response to initial stimuli, but due to changes in ion concentration, it is not suitable for describing the response to long-term stimuli.
Crafting Recognition: Understanding Gendered and Ethnicised Experiences in an Arts-Based Integration Project
Authors: Conard Stella Grace , Horton Elena
Published: October 2023

In Denmark, heightened public interest surrounding migration politics has become embodied in the arts, leading to the development of migration-related arts projects. In this study we explore the experiences of women taking part in an arts-based integration project designed for migrant and Danish women to knit, sew, and crochet in female company, with a view to professionalise their handicrafts. Our findings, which are grounded in ethnographic fieldwork as well as interviews with members of the group, demonstrate how handicraft acts as a prism through which categories such as gender, class, and ethnicity are negotiated within the project. We found that group members’ national and cultural backgrounds shaped their different expectations and experiences in the project. The roles they occupied and their self-perception within the group were also shaped by other factors, such as their family status, their state of employment, and whether handicraft was more of a ‘hobby’ or a source of income. The study makes a case for appreciating the importance of social recognition. Understanding how these women perceived their own and each other’s work becomes a magnifier of the socio-political context in which the integration project is situated. Artistic practice both enabled members to respond to an integration and refugee discourse, while simultaneously positioning them within such frameworks.
Performance Analysis of the Liquid Cooling System for Lithium-Ion Batteries According to Cooling Plate Parameters
Authors: You Nayoung , Ham Jeonggyun , Shin Donghyeon , Cho Honghyun
Published: October 2023

In this study, the effects of battery thermal management (BTM), pumping power, and heat transfer rate were compared and analyzed under different operating conditions and cooling configurations for the liquid cooling plate of a lithium-ion battery. The results elucidated that when the flow rate in the cooling plate increased from 2 to 6 L/min, the average temperature of the battery module decreased from 53.8 to 50.7 °C, but the pumping power increased from 0.036 to 0.808 W. In addition, an increase in the width of the cooling channel and number of channels resulted in a decrease in the average temperature of the battery module and a reduction in the pumping power. The most influential variable for the temperature control of the battery was an increase in the flow rate. In addition, according to the results of the orthogonal analysis, an increase in the number of cooling plate channels resulted in the best cooling performance and reduced pumping power. Based on this, a cooling plate with six channels was applied to both the top and bottom parts, and the top and bottom cooling showed sufficient cooling performance in maintaining the average temperature of the battery module below 45 °C.
Development of Novel Polysaccharide Membranes for Guided Bone Regeneration: In Vitro and In Vivo Evaluations
Authors: Ahmed Omar Naïma , Roque Jéssica , Galvez Paul , Siadous Robin , Chassande Olivier , Catros Sylvain , Amédée Joëlle , Roques Samantha , Durand Marlène , Bergeaut Céline , Bidault Laurent , Aprile Paola , Letourneur Didier , Fricain Jean-Christophe , Fenelon Mathilde
Published: October 2023

Introduction: Guided bone regeneration (GBR) procedures require selecting suitable membranes for oral surgery. Pullulan and/or dextran-based polysaccharide materials have shown encouraging results in bone regeneration as bone substitutes but have not been used to produce barrier membranes. The present study aimed to develop and characterize pullulan/dextran-derived membranes for GBR. Materials and methods: Two pullulan/dextran-based membranes, containing or not hydroxyapatite (HA) particles, were developed. In vitro, cytotoxicity evaluation was performed using human bone marrow mesenchymal stem cells (hBMSCs). Biocompatibility was assessed on rats in a subcutaneous model for up to 16 weeks. In vivo, rat femoral defects were created on 36 rats to compare the two pullulan/dextran-based membranes with a commercial collagen membrane (Bio-Gide®). Bone repair was assessed radiologically and histologically. Results: Both polysaccharide membranes demonstrated cytocompatibility and biocompatibility. Micro-computed tomography (micro-CT) analyses at two weeks revealed that the HA-containing membrane promoted a significant increase in bone formation compared to Bio-Gide®. At one month, similar effects were observed among the three membranes in terms of bone regeneration. Conclusion: The developed pullulan/dextran-based membranes evidenced biocompatibility without interfering with bone regeneration and maturation. The HA-containing membrane, which facilitated early bone regeneration and offered adequate mechanical support, showed promising potential for GBR procedures.
Calcium Prevents Enhanced Degradation of Factor VIII in the Condition of Motion
Authors: Cohen Haim , Keren-Politansky Anat , Crispel Yonatan , Yanovich Chen , Asayag Keren , Nadir Yona
Published: October 2023

Background: Hemophilia A and B induce recurrent bleeding episodes, mainly in skeletal muscles and joints that are in intermittent motion. We have previously demonstrated that intermittent motion contributes to increased degradation of factors VIII and IX. Objectives: Given that calcium ions are known to enhance factor VIII–von Willebrand factor (vWF) interaction, the present study has investigated the role of these ions on factors VIII and IX in the condition of motion. Methods: The effects of calcium ions were assessed using purified proteins via Western blot, factor VIII activity, immunocytochemistry, and in Institute of Cancer Research (ICR) mice with no specific genetic background. Results: Calcium was found to prevent degradation of plasma-derived factor VIII but not that of factor IX, during intermittent motion. Calcium levels in the microcirculation of mouse striated muscles were elevated following movement, enabling prevention of factor VIII degradation in normal physiology. Calcium supplementation in drinking water increased factor VIII levels in blood and striated muscles of ICR mice during movement. Conclusions: calcium ions decrease factor VIII degradation in the condition of motion. Further research on the impact of calcium salt oral supplementation on hemophilia patients is warranted.
Hope and Challenges: Immunotherapy in EGFR-Mutant NSCLC Patients
Authors: Yan Dan
Published: October 2023

EGFR tyrosine kinase inhibitors (TKIs) are the preferred initial treatment for non-small cell lung cancer (NSCLC) patients harboring sensitive EGFR mutations. Sadly, remission is transient, and no approved effective treatment options are available for EGFR-TKI-advanced EGFR-mutant NSCLCs. Although immunotherapy with immune checkpoint inhibitors (ICIs) induces sustained cancer remission in a subset of NSCLCs, ICI therapy exhibits limited activity in most EGFR-mutant NSCLCs. Mechanistically, the strong oncogenic EGFR signaling in EGFR-mutant NSCLCs contributes to a non-inflamed tumor immune microenvironment (TIME), characterized by a limited number of CD8+ T cell infiltration, a high number of regulatory CD4+ T cells, and an increased number of inactivated infiltrated T cells. Additionally, EGFR-mutant NSCLC patients are generally non-smokers with low levels of PD-L1 expression and tumor mutation burden. Promisingly, a small population of EGFR-mutant NSCLCs still durably respond to ICI therapy. The hope of ICI therapy from pre-clinical studies and clinical trials is reviewed in EGFR-mutant NSCLCs. The challenges of application ICI therapy in EGFR-mutant NSCLCs are also reviewed.
A Systematic Review of Deep-Learning Methods for Intracranial Aneurysm Detection in CT Angiography
Authors: Bizjak Žiga , Špiclin Žiga
Published: October 2023

Background: Subarachnoid hemorrhage resulting from cerebral aneurysm rupture is a significant cause of morbidity and mortality. Early identification of aneurysms on Computed Tomography Angiography (CTA), a frequently used modality for this purpose, is crucial, and artificial intelligence (AI)-based algorithms can improve the detection rate and minimize the intra- and inter-rater variability. Thus, a systematic review and meta-analysis were conducted to assess the diagnostic accuracy of deep-learning-based AI algorithms in detecting cerebral aneurysms using CTA. Methods: PubMed (MEDLINE), Embase, and the Cochrane Library were searched from January 2015 to July 2023. Eligibility criteria involved studies using fully automated and semi-automatic deep-learning algorithms for detecting cerebral aneurysms on the CTA modality. Eligible studies were assessed using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines and the Quality Assessment of Diagnostic Accuracy Studies 2 (QUADAS-2) tool. A diagnostic accuracy meta-analysis was conducted to estimate pooled lesion-level sensitivity, size-dependent lesion-level sensitivity, patient-level specificity, and the number of false positives per image. An enhanced FROC curve was utilized to facilitate comparisons between the studies. Results: Fifteen eligible studies were assessed. The findings indicated that the methods exhibited high pooled sensitivity (0.87, 95% confidence interval: 0.835 to 0.91) in detecting intracranial aneurysms at the lesion level. Patient-level sensitivity was not reported due to the lack of a unified patient-level sensitivity definition. Only five studies involved a control group (healthy subjects), whereas two provided information on detection specificity. Moreover, the analysis of size-dependent sensitivity reported in eight studies revealed that the average sensitivity for small aneurysms (<3 mm) was rather low (0.56). Conclusions: The studies included in the analysis exhibited a high level of accuracy in detecting intracranial aneurysms larger than 3 mm in size. Nonetheless, there is a notable gap that necessitates increased attention and research focus on the detection of smaller aneurysms, the use of a common test dataset, and an evaluation of a consistent set of performance metrics.
Effects of Schlemm’s Canal Suture Implantation Surgery and Pilocarpine Eye Drops on Trabecular Meshwork Pulsatile Motion
Authors: Sang Qing , Du Rong , Xin Chen , Wang Ningli
Published: October 2023

The trabecular meshwork is an important structure in the outflow pathway of aqueous humor, and its movement ability directly affects the resistance of aqueous humor outflow, thereby affecting the steady state of intraocular pressure (IOP). (1) Objective: The purpose of this study was to preliminarily estimate the effects of pilocarpine eye drops and trabeculotomy tunneling trabeculoplasty (3T) on trabecular meshwork (TM) pulsatile motion via phase-sensitive optical coherence tomography (Phs-OCT). (2) Method: In a prospective single-arm study, we mainly recruited patients with primary open-angle glaucoma who did not have a history of glaucoma surgery, and mainly excluded angle closure glaucoma and other diseases that may cause visual field damage. The maximum velocity (MV) and cumulative displacement (CDisp) of the TM were quantified via Phs-OCT. All subjects underwent Phs-OCT examinations before and after the use of pilocarpine eye drops. Then, all subjects received 3T surgery and examinations of IOP at baseline, 1 day, 1 week, 1 month, 3 months, and 6 months post-surgery. Phaco-OCT examinations were performed at 3 and 6 months post-surgery, and the measurements were compared and analyzed. (3) Results: The MV of TM before and after the use of pilocarpine eye drops was 21.32 ± 2.63 μm/s and 17.00 ± 2.43 μm/s. The CDisp of TM before and after the use of pilocarpine eye drops was 0.204 ± 0.034 μm and 0.184 ± 0.035 μm. After the use of pilocarpine eye drops, both the MV and CDisp significantly decreased compared to those before use (p < 0.001 and 0.013, respectively). The IOP decreased from baseline at 22.16 ± 5.23 mmHg to 15.85 ± 3.71 mmHg after 3 months post-surgery and from 16.33 ± 2.51 mmHg at 6 months post-surgery, showing statistically significant differences (p < 0.001). The use of glaucoma medication decreased from baseline at 3.63 ± 0.65 to 1.17 ± 1.75 at 3 months and 1.00 ± 1.51 at 6 months post-surgery; the differences were statistically significant (p < 0.001). Additionally, there was no statistically significant difference in the MV between 3 and 6 months after surgery compared to baseline (p = 0.404 and 0.139, respectively). Further, there was no statistically significant difference in the CDisp between 3 and 6 months after surgery compared to baseline (p = 0.560 and 0.576, respectively) (4) Conclusions: After the preliminary study, we found that pilocarpine eye drops can attenuate TM pulsatile motion, and that 3T surgery may reduce IOP without affecting the pulsatile motion status of the TM.
Extended-Spectrum β-Lactamases (ESBL): Challenges and Opportunities
Authors: Husna Asmaul , Rahman Md. Masudur , Badruzzaman A. T. M. , Sikder Mahmudul Hasan , Islam Mohammad Rafiqul , Rahman Md. Tanvir , Alam Jahangir , Ashour Hossam M.
Published: October 2023

The rise of antimicrobial resistance, particularly from extended-spectrum β-lactamase producing Enterobacteriaceae (ESBL-E), poses a significant global health challenge as it frequently causes the failure of empirical antibiotic therapy, leading to morbidity and mortality. The E. coli- and K. pneumoniae-derived CTX-M genotype is one of the major types of ESBL. Mobile genetic elements (MGEs) are involved in spreading ESBL genes among the bacterial population. Due to the rapidly evolving nature of ESBL-E, there is a lack of specific standard examination methods. Carbapenem has been considered the drug of first choice against ESBL-E. However, carbapenem-sparing strategies and alternative treatment options are needed due to the emergence of carbapenem resistance. In South Asian countries, the irrational use of antibiotics might have played a significant role in aggravating the problem of ESBL-induced AMR. Superbugs showing resistance to last-resort antibiotics carbapenem and colistin have been reported in South Asian regions, indicating a future bleak picture if no urgent action is taken. To counteract the crisis, we need rapid diagnostic tools along with efficient treatment options. Detailed studies on ESBL and the implementation of the One Health approach including systematic surveillance across the public and animal health sectors are strongly recommended. This review provides an overview of the background, associated risk factors, transmission, and therapy of ESBL with a focus on the current situation and future threat in the developing countries of the South Asian region and beyond.
Various Biomimetics, Including Peptides as Antifungals
Authors: Efremenko Elena , Aslanli Aysel , Stepanov Nikolay , Senko Olga , Maslova Olga
Published: October 2023

Biomimetics, which are similar to natural compounds that play an important role in the metabolism, manifestation of functional activity and reproduction of various fungi, have a pronounced attraction in the current search for new effective antifungals. Actual trends in the development of this area of research indicate that unnatural amino acids can be used as such biomimetics, including those containing halogen atoms; compounds similar to nitrogenous bases embedded in the nucleic acids synthesized by fungi; peptides imitating fungal analogs; molecules similar to natural substrates of numerous fungal enzymes and quorum-sensing signaling molecules of fungi and yeast, etc. Most parts of this review are devoted to the analysis of semi-synthetic and synthetic antifungal peptides and their targets of action. This review is aimed at combining and systematizing the current scientific information accumulating in this area of research, developing various antifungals with an assessment of the effectiveness of the created biomimetics and the possibility of combining them with other antimicrobial substances to reduce cell resistance and improve antifungal effects.
Elucidating the Influence of Lipid Composition on Bilayer Perturbations Induced by the N-Terminal Region of the Huntingtin Protein
Authors: Gamage Yasith Indigahawela , Pan Jianjun
Published: October 2023

Understanding the membrane interactions of the N-terminal 17 residues of the huntingtin protein (HttN) is essential for unraveling its role in cellular processes and its impact on huntingtin misfolding. In this study, we used atomic force microscopy (AFM) to examine the effects of lipid specificity in mediating bilayer perturbations induced by HttN. Across various lipid environments, the peptide consistently induced bilayer disruptions in the form of holes. Notably, our results unveiled that cholesterol enhanced bilayer perturbation induced by HttN, while phosphatidylethanolamine (PE) lipids suppressed hole formation. Furthermore, anionic phosphatidylglycerol (PG) and cardiolipin lipids, along with cholesterol at high concentrations, promoted the formation of double-bilayer patches. This unique structure suggests that the synergy among HttN, anionic lipids, and cholesterol can enhance bilayer fusion, potentially by facilitating lipid intermixing between adjacent bilayers. Additionally, our AFM-based force spectroscopy revealed that HttN enhanced the mechanical stability of lipid bilayers, as evidenced by an elevated bilayer puncture force. These findings illuminate the complex interplay between HttN and lipid membranes and provide useful insights into the role of lipid composition in modulating membrane interactions with the huntingtin protein.
Bacterial Proteases as Potentially Exploitable Modulators of SARS-CoV-2 Infection: Logic from the Literature, Informatics, and Inspiration from the Dog
Authors: Lushington Gerald H. , Linde Annika , Melgarejo Tonatiuh
Published: October 2023

(1) Background: The COVID-19 pandemic left many intriguing mysteries. Retrospective vulnerability trends tie as strongly to odd demographics as to exposure profiles, genetics, health, or prior medical history. This article documents the importance of nasal microbiome profiles in distinguishing infection rate trends among differentially affected subgroups. (2) Hypothesis: From a detailed literature survey, microbiome profiling experiments, bioinformatics, and molecular simulations, we propose that specific commensal bacterial species in the Pseudomonadales genus confer protection against SARS-CoV-2 infections by expressing proteases that may interfere with the proteolytic priming of the Spike protein. (3) Evidence: Various reports have found elevated Moraxella fractions in the nasal microbiomes of subpopulations with higher resistance to COVID-19 (e.g., adolescents, COVID-19-resistant children, people with strong dietary diversity, and omnivorous canines) and less abundant ones in vulnerable subsets (the elderly, people with narrower diets, carnivorous cats and foxes), along with bioinformatic evidence that Moraxella bacteria express proteases with notable homology to human TMPRSS2. Simulations suggest that these proteases may proteolyze the SARS-CoV-2 spike protein in a manner that interferes with TMPRSS2 priming.
Blockchain Technology Application in Security: A Systematic Review
Authors: Moosavi Nazanin , Taherdoost Hamed
Published: October 2023

Blockchain technology is a promising technology that attracts popularity among researchers. However, it was first introduced with digital currencies, particularly Bitcoin, but nowadays, it is also known as one of the most frequently used techniques for securing networks. This systematic review research identifies studies that use blockchain for their security challenges. In addition, different fields in blockchain usage, blockchain categorization type, consensus mechanism, smart contract usage, and integration with other software-based algorithms are also investigated. Our results maintain that the Internet of Things (IoT) is the main field in which blockchain provides security.
Effectiveness of Vestibular Rehabilitation for Patients with Degenerative Cerebellar Ataxia: A Retrospective Cohort Study
Authors: Heusel-Gillig Lisa L. , Hall Courtney D.
Published: October 2023

Many patients with cerebellar ataxia have dizziness caused by oculomotor or peripheral vestibular deficits; however, there is little evidence supporting the use of vestibular rehabilitation for this population. The purpose of this study was to determine whether patients with degenerative cerebellar diseases improve following rehabilitation including vestibular exercises. A secondary aim was to identify variables associated with the outcomes. A retrospective chart review identified 42 ambulatory patients (23 men and 19 women; mean age = 54.5 ± 14.4 years) with cerebellar degeneration. Fourteen patients had ataxia only, twenty had ataxia and oculomotor abnormalities, and eight had ataxia with oculomotor and peripheral vestibular deficits. Patients received customized physical therapy including balance and gait training, as well as gaze stabilization and habituation exercises for vestibular hypofunction and motion-provoked dizziness. Primary outcome measures (Activities-specific Balance Confidence Scale, Tinetti Performance Oriented Mobility Assessment, Dynamic Gait index, and Sensory Organization Test) were evaluated at baseline and discharge. Patients improved (p < 0.05) on all outcome measures. Patients with vestibular deficits were seen for more visits compared to those with gait ataxia only (7.1 vs. 4.8 visits). This study provides evidence that patients with degenerative cerebellar disease improve in balance confidence, fall risk and sensory integration with therapy that includes vestibular rehabilitation.
High Variability Periods in the EEG Distinguish Cognitive Brain States
Authors: Parameshwaran Dhanya , Thiagarajan Tara C.
Published: October 2023

Objective: To describe a novel measure of EEG signal variability that distinguishes cognitive brain states. Method: We describe a novel characterization of amplitude variability in the EEG signal termed “High Variability Periods” or “HVPs”, defined as segments when the standard deviation of a moving window is continuously higher than the quartile cutoff. We characterize the parameter space of the metric in terms of window size, overlap, and threshold to suggest ideal parameter choice and compare its performance as a discriminator of brain state to alternate single channel measures of variability such as entropy, complexity, harmonic regression fit, and spectral measures. Results: We show that the average HVP duration provides a substantially distinct view of the signal relative to alternate metrics of variability and, when used in combination with these metrics, significantly enhances the ability to predict whether an individual has their eyes open or closed and is performing a working memory and Raven’s pattern completion task. In addition, HVPs disappear under anesthesia and do not reappear in early periods of recovery. Conclusions: HVP metrics enhance the discrimination of various brain states and are fast to estimate. Significance: HVP metrics can provide an additional view of signal variability that has potential clinical application in the rapid discrimination of brain states.
Do Laparoscopic Approaches Ensure Oncological Safety and Prognosis for Serosa-Exposed Colon Cancer? A Comparative Study against the Open Approach
Authors: Seo Ji-Hyun , Park In-Ja
Published: October 2023

The adoption of laparoscopic surgery in the management of serosa-exposed colorectal cancer has raised concerns. This study aimed to investigate whether laparoscopic surgery is associated with an increased risk of postoperative recurrence in patients undergoing resection for serosa-exposed colon cancer. A retrospective analysis was conducted on a cohort of 315 patients who underwent curative resection for pathologically confirmed T4a colon cancer without distant metastases at the Asan Medical Center between 2006 and 2015. Patients were categorized according to the surgical approach method: laparoscopic surgery (MIS group) versus open surgery (Open group). Multivariate analysis was employed to identify risk factors associated with overall survival (OS) and disease-free survival (DFS). The MIS group included 148 patients and the Open group had 167 patients. Of the total cohort, 106 patients (33.7%) experienced recurrence during the follow-up period. Rates, patterns, and time to recurrence were not different between groups. The MIS group (55.8%) showed more peritoneal metastasis compared to the Open group (44.4%) among recurrence sites, but it was not significant (p = 0.85). There was no significant difference in the five-year OS (73.5% vs. 78.4% p = 0.374) or DFS (62.0% vs. 64.6%; p = 0.61) between the Open and MIS groups. Age and the pathologic N stage were independently associated with OS, and the pathologic N stage was the only associated risk factor for DFS. The laparoscopic approach for serosa-exposed colon cancer did not compromise the DFS and OS. This study provides evidence that laparoscopic surgery does not compromise oncologic outcomes of patients with T4a colon cancer although peritoneal seeding is the most common type of disease failure of serosa-exposed colon cancer.
Recent Advances in g-C3N4 Photocatalysts: A Review of Reaction Parameters, Structure Design and Exfoliation Methods
Authors: Pei Junxiang , Li Haofeng , Zhuang Songlin , Zhang Dawei , Yu Dechao
Published: October 2023

Graphitized carbon nitride (g-C3N4), as a metal-free, visible-light-responsive photocatalyst, has a very broad application prospect in the fields of solar energy conversion and environmental remediation. The g-C3N4 photocatalyst owns a series of conspicuous characteristics, such as very suitable band structure, strong physicochemical stability, abundant reserves, low cost, etc. Research on the g-C3N4 or g-C3N4-based photocatalysts for real applications has become a competitive hot topic and a frontier area with thousands of publications over the past 17 years. In this paper, we carefully reviewed the recent advances in the synthesis and structural design of g-C3N4 materials for efficient photocatalysts. First, the crucial synthesis parameters of g-C3N4 were fully discussed, including the categories of g-C3N4 precursors, reaction temperature, reaction atmosphere and reaction duration. Second, the construction approaches of various nanostructures were surveyed in detail, such as hard and soft template, supramolecular preorganization and template-free approaches. Third, the characteristics of different exfoliation methods were compared and summarized. At the end, the problems of g-C3N4 materials in photocatalysis and the prospect of further development were disclosed and proposed to provide some key guidance for designing more efficient and applicable g-C3N4 or g-C3N4-based photocatalysts.
Machine Learning Analysis of Alzheimer’s Disease Single-Cell RNA-Sequencing Data across Cortex and Hippocampus Regions
Authors: Krokidis Marios G. , Vrahatis Aristidis G. , Lazaros Konstantinos , Skolariki Konstantina , Exarchos Themis P. , Vlamos Panagiotis
Published: October 2023

Advancements in molecular biology have revolutionized our understanding of complex diseases, with Alzheimer’s disease being a prime example. Single-cell sequencing, currently the most suitable technology, facilitates profoundly detailed disease analysis at the cellular level. Prior research has established that the pathology of Alzheimer’s disease varies across different brain regions and cell types. In parallel, only machine learning has the capacity to address the myriad challenges presented by such studies, where the integration of large-scale data and numerous experiments is required to extract meaningful knowledge. Our methodology utilizes single-cell RNA sequencing data from healthy and Alzheimer’s disease (AD) samples, focused on the cortex and hippocampus regions in mice. We designed three distinct case studies and implemented an ensemble feature selection approach through machine learning, also performing an analysis of distinct age-related datasets to unravel age-specific effects, showing differential gene expression patterns within each condition. Important evidence was reported, such as enrichment in central nervous system development and regulation of oligodendrocyte differentiation between the hippocampus and cortex of 6-month-old AD mice as well as regulation of epinephrine secretion and dendritic spine morphogenesis in 15-month-old AD mice. Our outcomes from all three of our case studies illustrate the capacity of machine learning strategies when applied to single-cell data, revealing critical insights into Alzheimer’s disease.
Pearl Millet-Cowpea Forage Mixture Planting Arrangement Influences Mixture Yield and Nutritive Value in Semiarid Regions
Authors: Lauriault Leonard M. , Darapuneni Murali K. , Martinez Gasper K.
Published: October 2023

Pearl millet (Pennisetum glaucum (L.) R. Br.) and cowpea (Vigna unguiculata L. Walp.) are well-adapted to semiarid regions. A two-year study at New Mexico State University’s Rex E. Kirksey Agricultural Science Center at Tucumcari, NM, USA, compared monoculture pearl millet and cowpea with their mixtures in various row arrangements in four randomized complete blocks each year. Treatments included monoculture pearl millet (millet) and cowpea (cowpea), pearl millet and cowpea mixture planted in the same row (millet–cowpea), the species planted in alternate rows (millet–cowpea 1:1), the species planted in two adjacent rows alternating between species (millet–cowpea 2:2), and the species planted in four adjacent rows alternating between species (millet–cowpea 4:4), Mixture neutral detergent fiber (NDF) was reduced i millet–cowpea 1:1 and millet–cowpea 2:2 compared to millet (673, 662, 644, 646, and 666 g NDF kg−1 for millet, millet–cowpea, millet–cowpea 1:1, millet–cowpea 2:2, and millet–cowpea 4:4, respectively, LSD = 18, p ≤ 0.05). Crude protein tended to be increased in millet–cowpea 2:2. Based on these results two rows of cowpea alternated with two rows of pearl millet, all spaced at 15 cm and harvesting for hay at the pearl millet boot stage likely optimizes the compromise of DM yield and the nutritive value of the mixture.
Re-Evaluating Chemotherapy Dosing Strategies for Ovarian Cancer: Impact of Sarcopenia
Authors: Shah Rushi , Polen-De Clarissa , McGree Michaela , Fought Angela , Kumar Amanika
Published: October 2023

We investigated the impact of sarcopenia on adjuvant chemotherapy dosing in advanced epithelial ovarian cancer (EOC). The chemotherapy dosing and toxicity of 173 eligible patients who underwent cytoreductive surgery and adjuvant chemotherapy at a single institution were analyzed. Patients with a skeletal muscle index less than 39 cm2/m2 measured on a CT scan were considered sarcopenic. Sarcopenic and non-sarcopenic patients were compared with regard to relative dose intensity (RDI), completion of scheduled chemotherapy, toxicity, and survival. A total of 62 (35.8%) women were sarcopenic. Sarcopenic women were less likely to complete at least six cycles of chemotherapy (83.9% vs. 95.5%, p = 0.02). The mean RDI for both carboplatin (80.4% vs. 89.4%, p = 0.03) and paclitaxel (91.9% vs. 104.1%, p = 0.03) was lower in sarcopenic patients compared to non-sarcopenic patients. Despite these differences in chemotherapy, there was no difference in neutropenia or median overall survival (3.99 vs. 4.57 years, p = 0.62) between the sarcopenic and non-sarcopenic women, respectively. This study highlights the importance of considering lean body mass instead of body weight or surface area in chemotherapy dosing formulas for sarcopenic women with advanced EOC. Further research is needed to optimize chemotherapy strategies based on individual body composition, potentially leading to improved dosing strategies in this population.
Possible Reasons Affecting Different Phytophthora infestans Populations in Tomato and Potato Isolates in Thailand
Authors: Srisawad Nattaya , Petchaboon Kamonsiri , Sraphet Supajit , Tappiban Piengtawan , Triwitayakorn Kanokporn
Published: October 2023

Late blight, caused by the oomycete Phytophthora infestans, is one of the most important diseases affecting tomato and potato production worldwide. In Thailand, the disease is widespread in the north and northeast, especially in the Chiang-Mai and Tak provinces. The mating type, metalaxyl sensitivity, mitochondrial DNA (mtDNA) haplotype, RG57 fingerprinting, and microsatellite were used to characterize the P. infestans populations. The study revealed that the P. infestans of tomato isolates in Thailand are of the same lineage as those from 1994 until 2002. The clonal lineages that were found in the potato populations have changed since 1994. The changes in P. infestans isolates in the potato populations have likely been the result of the import of seed potatoes to Thailand. Furthermore, the P. infestans populations in potatoes show resistance to metalaxyl, whereas those from tomato isolates show sensitivity to fungicides. The reasons for the different responses can be attributed to (i) the use of metalaxyl, (ii) the host preferences of P. infestans, and (iii) the migration of new genotypes from infected potato seeds.