bluuebunny/crossref_metadata_2025 huggingface dataset:
Entrepreneurial Stewardship: Why Some Profits Should Be Used to Benefit Others
Authors: Lee Jooho
Published: March 2020

Entrepreneurs should act as stewards of entrepreneurial rent. Entrepreneurial rent is the difference between the ex post value of a venture and its ex ante costs. It is the result of competition among buyers and sellers within the market process rather than the sole efforts of the entrepreneur. As a result, entrepreneurs should allocate entrepreneurial rent for the benefit of other market participants rather than consuming it for themselves. The moral obligation to steward entrepreneurial rent is consistent with traditional bases of property rights and the norm of social welfare maximization, and it applies to corporations and their shareholders, as well as individual entrepreneurs.
Organic selenium supplement partially alleviated diquat-induced oxidative insults and hepatic metabolic stress in nursery pigs
Authors: Doan Nicole , Liu Yanhong , Xiong Xia , Kim Kwangwook , Wu Zhaohai , Bravo David M. , Blanchard Alexandra , Ji Peng
Published: March 2020

The study investigated antioxidant effects of Se on resilience to diquat-induced oxidative stress in nursery pigs. Thirty-five weaned pigs were individually housed and randomly assigned to one of the five treatments. Pigs were (1) fed a basal diet and intraperitoneally injected with sterile saline (negative control), (2) fed the basal diet and injected with diquat solution (positive control, PC), or fed the basal diet supplemented with 0·3 mg Se/kg as (3) sodium selenite (SS), (4) soyabean protein-chelated Se (SC) or (5) selenised yeast (SY) and intraperitoneally injected with diquat. Pigs were fed the experimental diets for 17 d and injected with diquat at 10 mg/kg body weight or saline on the 11th day of the study (day 0 post-injection (PI)). Diquat exposure induced acute stress and innate immune activation (P< 0·05) at 6 h PI and compromised (P< 0·05) plasma glutathione peroxidase activity on day 2 PI, which was accompanied by an increase in plasma malondialdehyde at 6 h and day 2 PI (P< 0·10). Organic Se, particularly SY, enhanced (P< 0·05) endogenous antioxidant activity in various aspects compared with the PC group. The growth rate and feed intake from day 0 to day 7 PI were significantly lower in the PC, SS and SC groups than the NC group (P< 0·05). Untargeted metabolomics analysis revealed that twenty-two hepatic metabolites (false discovery rate < 0·15) associated with lipid and cellular antioxidant metabolism were altered by diquat. SY restored hepatic metabolic profiles in some but not all samples.
Otolaryngology residents’ perceptions of endoscopic ear surgery during surgical training
Authors: Ng C L , Ong W , Ngo R Y S
Published: March 2020

Background

The introduction of endoscopic ear surgery has implications for the training of otolaryngology residents.

Objectives

To report on the status of endoscopic ear surgery and assess the effects of this new technology on otolaryngology training in Singapore, from the residents’ perspective.

Methods

An anonymous survey was conducted amongst all Singaporean otolaryngology residents. Residents’ exposure to, and perceptions of, endoscopic ear surgery were assessed.

Results

Residents from institutions that practise endoscopic ear surgery were more positive regarding its efficacy in various otological surgical procedures. Of residents in programmes with exposure to endoscopic ear surgery, 82.4 per cent felt that its introduction had adversely affected their training, with 88.3 per cent of residents agreeing that faculty members’ learning of endoscopic ear surgery had decreased their hands-on surgical load. Both groups expressed desire for more experience with endoscopy.

Conclusion

The majority of residents view endoscopic ear surgery as an expanding field with a potentially negative impact on their training. Mitigating measures should be implemented to minimise its negative impact on residents’ training.
Reading Differences: The Odyssey and Juxtaposition
Authors: Goldhill Simon
Published: March 2020

This article comprises a discussion of four separate passages in Homer and some of the critical problems which each involves. My intention is not to produce a blueprint or set of rules for the interpretation of Homer, but rather — a more limited aim — to increase attention to the complex texture of the poetry of theOdyssey, and to the need for a critical practice alive to such complexity. The four passages are the speech of Amphimedon's ghost; the recognition scene between Odysseus and Argus; the story telling of Menelaus and Helen; and, finally, Odysseus' first speech to Nausicaa. Each passage opens questions about how Homer is read, and, in particular, about how what is often referred to as Homer's juxtapositional technique interrelates with the role of the reader in the activity of interpretation.
Marketization and Its Discontents: Unveiling the Impacts of Foundation-led Venture Philanthropy on Grassroots NGOs in China
Authors: Lai Weijun , Spires Anthony J.
Published: March 2020

Although the Chinese state has an outsized influence on shaping civil society in China, extant literature has generally overlooked the increasing role of the market in its non-governmental organization (NGO) development. This paper examines the marketization of Chinese civil society through an ethnographic investigation of funding relationships between domestic Chinese philanthropic foundations and grassroots NGOs. Two case studies of foundation venture philanthropy projects show that businesspeople, through their intensive involvement in foundation-led funding programmes, are introducing strong market influences to the non-profit sector. Notwithstanding the attraction of foundation funding, many NGOs decry the negative side effects of non-profit marketization. We argue that NGOs in this context risk being transformed into social product providers and resource-chasing machines, detracting from the self-directed social missions that many NGO leaders see as their original calling. These observations on emergent NGO–foundation relationships also reflect participants’ increasing uncertainty about the direction of Chinese civil society development.
Plague, pumas and potential zoonotic exposure in the Greater Yellowstone Ecosystem
Authors: Elbroch L Mark , Vickers T Winston , Quigley Howard B
Published: March 2020

We tested for plague (Yersinia pestis) in a puma population in the Greater Yellowstone Ecosystem (GYE) over 9 years, overlapping a case when a boy in the area became infected with plague. Antibodies to Y. pestis were detected in 8 of 17 (47%) pumas tested by complement-enzyme-linked immunosorbent assay, and the organism itself was detected in 4 of 11 (36%) pumas tested after necropsy. Neither puma sex nor age was significantly associated with Y. pestis exposure or mortality, although our sample size was small. The overall prevalence of exposure we recorded was similar to that found along the western slope of Colorado, which is adjacent to the Four Corners region, a known plague hotspot in the USA. This suggests that: (1) Y. pestis may be present at higher levels in the GYE than previously assumed; (2) plague is a significant source of mortality for local pumas (6.6% of sub-adult and adult mortality); and (3) pumas may be a useful sentinel for potential risk of plague exposure to humans throughout the West. We would also emphasize that hunters and others handling pumas in this region should be made aware of the possibility of exposure.
Dry forest is more threatened but less protected than evergreen forest in Ecuador’s coastal region
Authors: Rivas Carlos A , Navarro-Cerillo Rafael M , Johnston Jon C , Guerrero-Casado José
Published: March 2020

The Ecuadorian coast has two main types of forests, which are differentiated by their phenology: dry forests are deciduous and more humid forests are evergreen. Less of the dry forests on the Ecuadorian coast is protected (13% of its area) than the evergreen forests (28%), and the area devoted to the protection of dry forests (1069 km2) is substantially less than the area devoted to the protection of evergreen forests (2800 km2). Yet the conservation status of dry forests is more critical, with 83% of their area classified as having very low connectivity, 70% as being highly fragile and 86% as being highly threatened. In addition, the dry forests have fewer protected areas than the evergreen forests. It is therefore necessary to increase the protection of deciduous ecosystems as part of a comprehensive national strategy because they support high levels of biodiversity and many endemic species.
Potential distribution of a climate sensitive species, the White-winged Snowfinch Montifringilla nivalis in Europe
Authors: BRAMBILLA MATTIA , RESANO-MAYOR JAIME , ARLETTAZ RAPHAËL , BETTEGA CHIARA , BINGGELI ANAÏS , BOGLIANI GIUSEPPE , BRAUNISCH VERONIKA , CELADA CLAUDIO , CHAMBERLAIN DAN , CHIFFARD CARRICABURU JULES , DELGADO MARIA DEL MAR , FONTANILLES PHILIPPE , KMECL PRIMOŽ , KORNER FRÄNZI , LINDNER ROBERT , PEDRINI PAOLO , PÖHACKER JAKOB , RUBINIČ BORUT , SCHANO CHRISTIAN , SCRIDEL DAVIDE , STRINELLA ELISEO , TEUFELBAUER NORBERT , DE GABRIEL HERNANDO MIGUEL
Published: March 2020

The White-winged Snowfinch Montifringilla nivalis nivalis is assumed to be highly threatened by climate change, but this high elevation species has been little studied and the current breeding distribution is accurately known only for a minor portion of its range. Here, we provide a detailed and spatially explicit identification of the potentially suitable breeding areas for the Snowfinch. We modelled suitable areas in Europe and compared them with the currently known distribution. We built a distribution model using 14,574 records obtained during the breeding period that integrated climatic, topographic and land-cover variables, working at a 2-km spatial resolution with MaxEnt. The model performed well and was very robust; average annual temperature was the most important occurrence predictor (optimum between c.-3°C and 0°; unsuitable conditions below -10° and above 5°). The current European breeding range estimated by BirdLife International was almost three times greater than that classified as potentially suitable by our model. Discrepancies between our model and the distribution estimated by BirdLife International were particularly evident in eastern Europe, where the species is poorly monitored. Southern populations are likely more isolated and at major risk because of global warming. These differences have important implications for the supposed national responsibility for conservation of the species and highlight the need for new investigations on the species in the eastern part of its European range.
Computing knowledge in equational extensions of subterm convergent theories
Authors: Erbatur Serdar , Marshall Andrew M. , Ringeissen Christophe
Published: March 2020

We study decision procedures for two knowledge problems critical to the verification of security protocols, namely the intruder deduction and the static equivalence problems. These problems can be related to particular forms of context matching and context unification. Both problems are defined with respect to an equational theory and are known to be decidable when the equational theory is given by a subterm convergent term rewrite system (TRS). In this work, we extend this to consider a subterm convergent TRS defined modulo an equational theory, like Commutativity. We present two pairs of solutions for these important problems. The first solves the deduction and static equivalence problems in rewrite systems modulo shallow theories such as Commutativity. The second provides a general procedure that solves the deduction and static equivalence problems in subterm convergent systems modulo syntactic permutative theories, provided a finite measure is ensured. Several examples of such theories are also given.
Research Infrastructures in Europe: The Hype and the Field
Authors: Hallonsten Olof
Published: March 2020

Research Infrastructures (RIs) are tools for scientific research that have received increased attention in science policy in Europe in recent years, including the launch of specific governance bodies and a structured process of prioritization and organization of RI projects in the making. But there is no commonly accepted definition of what RIs are, and the category is both very varied and lacks historical roots. This article provides a historical contextualization of this policy area and discusses, in some detail, different ways of categorizing the 60 RIs identified by EU-level governance bodies as strategically important for Europe as a whole. Showing that the concept of RIs is a political construct with little analytical value for assessing the role and function of RIs in science and innovation systems, the article paves the way for more conceptually and analytically stringent studies of the politics of RIs in Europe.
LitMod2D_2.0: An Improved Integrated Geophysical‐Petrological Modeling Tool for the Physical Interpretation of Upper Mantle Anomalies
Authors: Kumar Ajay , Fernàndez Manel , Jiménez‐Munt Ivone , Torne Montserrat , Vergés Jaume , Afonso Juan Carlos
Published: March 2020

LitMod2D integrates geophysical and petrological data sets to produce the thermal, density, and seismic velocity structure of the lithosphere and upper mantle. We present a new LitMod2D_2.0 package with improvements focused on (i) updated anelastic attenuation correction for anharmonic seismic velocities, (ii) chemical composition in the sublithospheric mantle, and (iii) incorporation of sublithospheric mantle anomalies. Sublithospheric mantle anomalies can be defined with different chemical composition, temperature, seismic velocities, and a combination of them, allowing the application of LitMod2D_2.0 to regions affected by mantle upwelling, subduction, delamination, and metasomatism. We demonstrate the potential application of LitMod2D_2.0 to such regions and the sensitivity of thermal and compositional anomalies on density and seismic velocities through synthetic models. Results show nonlinearity between the sign of thermal and seismic velocity anomalies, and that S wave velocities are more sensitive to temperature whereas P wave velocities are to composition. In a synthetic example of subduction, we show the sensitivity of sublithospheric mantle anomalies associated with the slab and the corner flow on surface observables (elevation, geoid height, and gravity anomalies). A new open‐source graphic user interface is incorporated in the new package. The output of the code is simplified by writing only the relevant physical parameters (temperature, pressure, material type, density, and seismic velocities) to allow the user using predefined post‐processing codes from a toolbox (flexure, mineral assemblages, synthetic passive seismological data, and tomography) or designing new ones. We demonstrate a post‐processing example calculating synthetic seismic tomography, Rayleigh surface‐wave dispersion curves, and P wave receiver functions from the output file of LitMod2D_2.0.
Estimation of the Critical Infiltration Rate for Air Compression During Infiltration
Authors: Wei Yunbo , Chen Kouping , Wu Jichun
Published: March 2020

During intense rainfall or flood irrigation, air may be compressed below the wetting front in the unsaturated zone, which reduces infiltration and thereby leads to excessive runoff and soil erosion. However, estimation of the critical infiltration rate (Icrit) that leads to air compression remains difficult. In this study, the critical infiltration rate for air compression was investigated experimentally and analytically. During infiltration experiments, significant air compression was observed as the infiltration rate excessed some critical rate for the working sands. And this measured critical rate increased as particle size of the sand increased. An innovative analytical approach was then proposed to estimate the values of Icrit using the parameters of the sands. When taking the impedance of air into consideration, the estimated Icrit matched well with the measured Icrit in the experiments. Based on the analytical approach, an equation was proposed to predict Icrit for a porous medium. According to the equation, the value of Icrit was determined by the permeability and pore size distribution of the porous medium. It was found that Icrit increased with the permeability of the porous medium, and also increased as the pore size of the medium became more uniform.
Thank You to Our 2019 Reviewers
Authors: Schildgen Taylor , Aitchison Jonathan , Jolivet Laurent , Rusmore Margi
Published: March 2020

Peer review is an essential component of scientific publishing, ensuring that our science is well communicated, accurately documented, appropriately placed in the context of prior work, and effectively archived for future usage, all within a timeframe that ensures the highest possible impact. The 200 papers published in Tectonics in 2019 benefitted from the careful scrutiny and constructive criticism drawn from the expertise of 728 reviewers, who provided a total of 1,082 reviews. These papers have helped to elucidate the evolution, structure, and deformation of Earth's lithosphere across a range of geologic to recent timescales. To all of the members of our community who have volunteered their time and efforts toward this collective goal, we thank you for helping Tectonics produce the high quality output that has helped us maintain a prominent position in scientific publishing for years, and for the spirit of teamwork that makes the peer review process an asset in our community.
Long noncoding RNA TRPM2-AS acts as a microRNA sponge of miR-612 to promote gastric cancer progression and radioresistance
Authors: Xiao Jian , Lin Linling , Luo Dakui , Shi Liang , Chen Wangwang , Fan Hao , Li Zengliang , Ma Xiang , Ni Peidong , Yang Li , Xu Zekuan
Published: March 2020

Long noncoding RNAs (lncRNAs) are emerging as important regulators of tumorigenesis and are frequently dysregulated in cancers. Here, we identify a critical lncRNA TRPM2-AS which is aberrantly expressed in gastric cancer (GC) tissues by screening The Cancer Genome Atlas Program(TCGA) database of GC cohort, and its upregulation is clinically associated with advanced pathologic stages and poor prognosis in GC patients. Silencing TRPM2-AS inhibits the proliferation, metastasis and radioresistance of GC cell whereas ectopic expression of TRPM2-AS significantly improves the progression of GC cell in multiple experiments. Mechanistically, TRPM2-AS serves as a microRNA sponge or a competitive endogenous RNA (ceRNA) for tumor suppressive microRNA miR-612 and consequently modulates the derepression of IGF2BP1 and FOXM1. Moreover, induced upregulation of IGF2BP1 subsequently increases the expression of c-Myc and promotes GC cell progression. Meanwhile, TRPM2-AS promotes the radioreistance of GC cell through enhancing the expression of FOXM1 as well. Thus, our findings support a new regulatory axis between TRPM2-AS, miR-612, IGF2BP1, or FOXM1 which serve as crucial effectors in GC tumorigenesis and malignant development, suggesting a promising therapeutic and diagnostic direction for GC.
Dual metabolic reprogramming by ONC201/TIC10 and 2-Deoxyglucose induces energy depletion and synergistic anti-cancer activity in glioblastoma
Authors: Pruss Maximilian , Dwucet Annika , Tanriover Mine , Hlavac Michal , Kast Richard Eric , Debatin Klaus-Michael , Wirtz Christian Rainer , Halatsch Marc-Eric , Siegelin Markus David , Westhoff Mike-Andrew , Karpel-Massler Georg
Published: March 2020

Background

Dysregulation of the metabolome is a hallmark of primary brain malignancies. In this work we examined whether metabolic reprogramming through a multi-targeting approach causes enhanced anti-cancer activity in glioblastoma.

Methods

Preclinical testing of a combined treatment with ONC201/TIC10 and 2-Deoxyglucose was performed in established and primary-cultured glioblastoma cells. Extracellular flux analysis was used to determine real-time effects on OXPHOS and glycolysis. Respiratory chain complexes were analysed by western blotting. Biological effects on tumour formation were tested on the chorioallantoic membrane (CAM).

Results

ONC201/TIC10 impairs mitochondrial respiration accompanied by an increase of glycolysis. When combined with 2-Deoxyglucose, ONC201/TIC10 induces a state of energy depletion as outlined by a significant decrease in ATP levels and a hypo-phosphorylative state. As a result, synergistic anti-proliferative and anti-migratory effects were observed among a broad panel of different glioblastoma cells. In addition, this combinatorial approach significantly impaired tumour formation on the CAM.

Conclusion

Treatment with ONC201/TIC10 and 2-Deoxyglucose results in a dual metabolic reprogramming of glioblastoma cells resulting in a synergistic anti-neoplastic activity. Given, that both agents penetrate the blood–brain barrier and have been used in clinical trials with a good safety profile warrants further clinical evaluation of this therapeutic strategy.
Mutations in the coat complex II component SEC23B promote colorectal cancer metastasis
Authors: Yang Chunyuan , Chen Nan , Li Xiang , Lu Dan , Hou Zhiyuan , Li Yuhua , Jin Yan , Gu Jin , Yin Yuxin
Published: March 2020

Metastasis is the leading cause of death for colorectal cancer (CRC). However, the protein transport process involved in CRC metastasis remains unclear. In this report, we use whole-exome sequencing and bioinformatics analysis to identify somatic mutations in CRC samples and found mutations of the protein transport gene Sec23 homolog B (SEC23B) in patients with metachronous liver metastasis. We show that deletion of SEC23B suppresses the membrane localization of adhesion proteins and augments cell mobility. SEC23B mutations either cause a premature stop (C649T) or impair its protein transport activity (C1467G and T488C + G791A + G2153A). Furthermore, SEC23B mutations inhibit the transport of epithelial cell adhesion molecule (EPCAM) and CD9 molecule, thereby attenuating cell adhesion and promoting invasiveness both in vitro and in vivo. Taken together, these data demonstrate the important impact of SEC23B mutations on metastasis, and we propose that SEC23B is a potential suppressor of CRC metastasis.
Reference values of body composition parameters and visceral adipose tissue (VAT) by DXA in adults aged 18–81 years—results from the LEAD cohort
Authors: Ofenheimer Alina , Breyer-Kohansal Robab , Hartl Sylvia , Burghuber Otto C. , Krach Florian , Schrott Andrea , Wouters Emiel F. M. , Franssen Frits M. E. , Breyer Marie-Kathrin
Published: March 2020

Background

Increasing attention has been drawn on the assessment of body composition phenotypes, since the distribution of soft tissue influences cardio-metabolic risk. Dual-energy X-ray absorptiometry (DXA) is a validated technique to assess body composition. European reference values from population-based cohorts are rare.

Aims

To provide age- and sex-related reference values of body composition parameters and visceral adipose tissue (VAT) mass, and for lean mass index (LMI) with regard to fat mass index (FMI) quantities and BMI categories.

Methods

GE-Lunar Prodigy DXA scans of 10.894 participants, aged 18–81 years, recruited from 2011 to 2019 by the Austrian LEAD study, a population-based cohort study, have been used to construct reference curves using the LMS method. Parameters assessed are FMI, LMI, appendicular LMI, fat mass ratios android/gynoid and trunk/limbs, and VAT.

Results

All lean mass and fat mass parameters indicating central fat accumulation were higher in men, whereas other fat mass indices were higher in women. LMI differed between each FMI subgroup (low vs. normal, low vs. high, normal vs. high), and BMI category in all ages and LMI increased with FMI and BMI classes. VAT mass was higher in men compared with women and increased across all age groups within both sexes.

Conclusion

The present study provides age- and sex-related reference values for European adults aged 18–81 years for body composition parameters and VAT mass for Lunar Prodigy DXA. In addition, this study reports LMI reference values with regard to fat mass quantities, showing a positive association with increasing FMI percentiles and BMI categories.
Parentage and relatedness reconstruction in Pinus sylvestris using genotyping-by-sequencing
Authors: Hall David , Zhao Wei , Wennström Ulfstand , Andersson Gull Bengt , Wang Xiao-Ru
Published: March 2020

Estimating kinship is fundamental for studies of evolution, conservation, and breeding. Genotyping-by-sequencing (GBS) and other restriction based genotyping methods have become widely applied in these applications in non-model organisms. However, sequencing errors, depth, and reproducibility between library preps could potentially hinder accurate genetic inferences. In this study, we tested different sets of parameters in data filtering, different reference populations and eight estimation methods to obtain a robust procedure for relatedness estimation in Scots pine (Pinus sylvestrisL.). We used a seed orchard as our study system, where candidate parents are known and pedigree reconstruction can be compared with theoretical expectations. We found that relatedness estimates were lower than expected for all categories of kinship estimated if the proportion of shared SNPs was low. However, estimates reached expected values if loci showing an excess of heterozygotes were removed and genotyping error rates were considered. The genetic variance-covariance matrix (G-matrix) estimation, however, performed poorly in kinship estimation. The reduced relatedness estimates are likely due to false heterozygosity calls. We analyzed the mating structure in the seed orchard and identified a selfing rate of 3% (including crosses between clone mates) and external pollen contamination of 33.6%. Little genetic structure was observed in the sampled Scots pine natural populations, and the degree of inbreeding in the orchard seed crop is comparable to natural stands. We illustrate that under our optimized data processing procedure, relatedness, and genetic composition, including level of pollen contamination within a seed orchard crop, can be established consistently by different estimators.
Transient genome-wide interactions of the master transcription factor NLP7 initiate a rapid nitrogen-response cascade
Authors: Alvarez José M. , Schinke Anna-Lena , Brooks Matthew D. , Pasquino Angelo , Leonelli Lauriebeth , Varala Kranthi , Safi Alaeddine , Krouk Gabriel , Krapp Anne , Coruzzi Gloria M.
Published: March 2020

Dynamic reprogramming of gene regulatory networks (GRNs) enables organisms to rapidly respond to environmental perturbation. However, the underlying transient interactions between transcription factors (TFs) and genome-wide targets typically elude biochemical detection. Here, we capture both stable and transient TF-target interactions genome-wide within minutes after controlled TF nuclear import using time-series chromatin immunoprecipitation (ChIP-seq) and/or DNA adenine methyltransferase identification (DamID-seq). The transient TF-target interactions captured uncover the early mode-of-action of NIN-LIKE PROTEIN 7 (NLP7), a master regulator of the nitrogen signaling pathway in plants. These transient NLP7 targets captured in root cells using temporal TF perturbation account for 50% of NLP7-regulated genes not detectably bound by NLP7 in planta. Rapid and transient NLP7 binding activates early nitrogen response TFs, which we validate to amplify the NLP7-initiated transcriptional cascade. Our approaches to capture transient TF-target interactions genome-wide can be applied to validate dynamic GRN models for any pathway or organism of interest.
Post-translational protein modifications in schizophrenia
Authors: Mueller Toni M. , Meador-Woodruff James H.
Published: March 2020

Research investigating the pathophysiology of schizophrenia has not yet precisely defined the molecular phenotype of this disorder. Many studies have investigated cellular dysfunction by examining expression levels of molecular targets in postmortem patient brain; however, inconsistencies between transcript and protein measures in schizophrenia are common in the field and represent a challenge to the identification of a unified model of schizophrenia pathogenesis. In humans, >4800 unique proteins are expressed, and the majority of these are modified by glycans and/or lipids. Estimates indicate ~70% of all eukaryotic proteins are modified by at least one type of glycosylation, while nearly 20% of all proteins are known to be lipid-modified. Protein post-translational modification (PTM) by glycosylation and lipidation rely on the spatiotemporal colocalization of enzyme, substrate, and glycan or lipid donor molecule and do not require an upstream “blueprint” or specialized processing machinery for synthesis. Glycan and lipid PTMs can thus facilitate cellular adaptation to environmental signals more rapidly than changes of gene or protein expression, and can significantly impact the localization, function, and interactions of modified substrates, though relatively few studies in schizophrenia have evaluated the PTM status of target proteins. A growing body of literature reports glycosylation and lipidation abnormalities in schizophrenia brain as well as in patient peripheral fluids. In this review, we explain the functional significance of key glycan and lipid PTMs and summarize current findings associated with abnormal glycosylation and lipidation in this illness.
Characterisation of serum progesterone and progesterone-induced blocking factor (PIBF) levels across trimesters in healthy pregnant women
Authors: Lim Mark Kit , Ku Chee Wai , Tan Thiam Chye , Lee Yin Hao Justin , Allen John Carson , Tan Nguan Soon
Published: March 2020

Progesterone-induced blocking factor (PIBF), which plays an important role in maintaining healthy pregnancies, has shown great promise as a prognostic biomarker for threatened miscarriage. To better characterise the physiological trends of progesterone and PIBF, we analysed serum progesterone and PIBF concentrations in healthy non-pregnant and pregnant women across trimesters. We saw increasing concentrations of progesterone and PIBF in pregnant women with advancing trimesters. The serum progesterone and PIBF percentiles across gestational age in healthy pregnancies can be used as a guide for the formulation of reference ranges. We also demonstrated a significant positive correlation between progesterone and PIBF levels. This study demonstrates increasing progesterone and PIBF concentrations in later trimesters and underscores the importance of progesterone and PIBF in healthy pregnancies. Characterisation of progesterone and PIBF across gestational age in healthy pregnant women may help to prognosticate pregnancy viability and support further research into the importance of progesterone and PIBF in the maintenance of healthy pregnancies.
Nutrients, minerals, pigments, phytochemicals, and radical scavenging activity in Amaranthus blitum leafy vegetables
Authors: Sarker Umakanta , Oba Shinya
Published: March 2020

A. blitum is good sources of abundant natural antioxidant phytopigments such as anthocyanin, betalain, betaxanthin, and betacyanin and antioxidant phytochemicals of interest in the food industry. The chances of utilizing amaranth pigments and phytochemicals had been evaluated for extracting colorful juice as drink purposes. Hence, the presence of nutrients, phytopigments, phytochemicals, and radical scavenging activity of selected A. blitum leafy vegetables were evaluated. Leaves of A. blitum have considerable fiber, moisture, protein, and carbohydrates. It has considerable magnesium, calcium, potassium (30.42, 24.74, 10.24 mg g−1), zinc, iron, copper, manganese, (878.98, 1153.83, 26.13, 207.50 µg g−1), phytopigments such as chlorophyll a, chlorophyll ab, chlorophyll b, (63.69, 90.60, 29.32 mg 100 g−1), betalain, betaxanthin, betacyanin (112.01, 58.38, 53.63 µg 100 g−1), vitamin C (1848.15 µg g−1), total carotenoids, β-carotene (1675.38, 1281.66 µg g−1), TPC, TFC (253.45 GAE and 162.97 RE µg g−1 DW), and TAC (29.46, 55.72 µg g−1 DW in Tolax equivalent DPPH and ABTS+ radical scavenging capacity) in A. blitum. The accessions DS3, DS6, DS8, and DS12 exhibited the highest TAC in Trolox equivalent DPPH and ABTS+ radical scavenging capacity, flavonoids, and considerable phytopigments. These accessions had excellent antioxidant profiles along with high yielding potentiality. Hence, A. blitum provides an excellent source of proximate, phenolics, minerals, flavonoids, vitamins, and phytopigments to address the nutritional and antioxidant deficiency in daily diet.
Identification and sequence analyses of the gliding machinery proteins from Mycoplasma mobile
Authors: Tulum Isil , Kimura Kenta , Miyata Makoto
Published: March 2020

Mycoplasma mobile, a fish pathogen, exhibits its own specialized gliding motility on host cells based on ATP hydrolysis. The special protein machinery enabling this motility is composed of surface and internal protein complexes. Four proteins, MMOBs 1630, 1660, 1670, and 4860 constitute the internal complex, including paralogs of F-type ATPase/synthase α and β subunits. In the present study, the cellular localisation for the candidate gliding machinery proteins, MMOBs 1620, 1640, 1650, and 5430 was investigated by using a total internal reflection fluorescence microscopy system after tagging these proteins with the enhanced yellow fluorescent protein (EYFP). The M. mobile strain expressing a fusion protein MMOB1620-EYFP exhibited reduced cell-binding activity and a strain expressing MMOB1640 fused with EYFP exhibited increased gliding speed, showing the involvement of these proteins in the gliding mechanism. Based on the genomic sequences, we analysed the sequence conservativity in the proteins of the internal and the surface complexes from four gliding mycoplasma species. The proteins in the internal complex were more conserved compared to the surface complex, suggesting that the surface complex undergoes modifications depending on the host. The analyses suggested that the internal gliding complex was highly conserved probably due to its role in the motility mechanism.
Drone Surveys Do Not Increase Colony-wide Flight Behaviour at Waterbird Nesting Sites, But Sensitivity Varies Among Species
Authors: Barr Jared R. , Green M. Clay , DeMaso Stephen J. , Hardy Thomas B.
Published: March 2020

The popularity of using unmanned aerial vehicles (UAVs) to survey colonial waterbirds has increased in the past decade, but disturbance associated with this bourgeoning technology requires further study. Disturbance was investigated by conducting aerial surveys with a consumer-grade quadcopter (DJI Phantom 3), while concurrently recording behavioural reactions on video. Surveys of mixed-species waterbird colonies (1-6 species per colony) were flown in horizontal transects at heights of 122, 91, 61, and 46 m, which is a typical range for collecting aerial imagery and producing high-resolution mosaicked orthophotos of nesting bird sites. An upper limit of 122 m was used due to local regulations prohibiting higher-altitude flights without federal authorization. Behavioural reactions were tallied every minute and a disturbance score was calculated for each sampling period. When compared to control periods, we found no evidence that colony-wide escape (i.e., flight) behaviour increased during drone flights, at any altitude flown. However, disturbance score increased significantly by 53% for surveys at 46 m. Some species were more sensitive to surveys than others. Laughing Gulls, in particular, exhibited a significant (125%) increase in escape behaviour for surveys at 91 m. Our results indicate when used in a capacity to gather high-resolution imagery for estimating breeding pairs, UAV surveys affected some species more than others, but severe reactions did not appear to increase for mixed-species colonies as a whole. Further study on safe operating thresholds is essential, especially at local and regional scales.
CVD-Grown Carbon Nanotube Branches on Black Silicon Stems for Ultrahigh Absorbance in Wide Wavelength Range
Authors: Phan Thanh Luan , Yu Woo Jong
Published: March 2020

We report a black silicon-carbon nanotube (bSi-CNT) hybrid structure for ultrahigh absorbance at wide spectral range of wavelength (300–1200 nm). CNTs are densely grown on entire bSi stems by chemical vapor deposition (CVD) through uniformly coating Fe catalyst. The bSi-CNT not only increases the surface roughness for enhancing the light suppression, but also allows the absorption of light in a wide wavelength range over the Si band gap (>1000 nm owing to 1.1 eV) due to the small band gap of CNT (0.6 eV). At short wavelength below Si band gap (<1000 nm), the absorbance of bSi-CNT shows average of 98.1%, while bSi shows 89.4%, which is because of high surface roughness of bSi-CNT that enhancing the light trapping. At long wavelength over Si band gap, the absorbance of bSi-CNT was maintained to 96.3% because of the absorption in CNT, while absorbance of bSi abruptly reduces with increase wavelength. Especially, the absorbance of bSi-CNT was showed 93.5% at 1200 nm, which is about 30~90% higher than previously reported bSi. Simple growth of CNTs on bSi can dramatically enhances the absorbance without using any antireflection coating layer. Thus, this study can be employed for realizing high efficiency photovoltaic, photocatalytic applications.
Hepatic stellate cell hypertrophy is associated with metabolic liver fibrosis
Authors: Hoffmann Céline , Djerir Nour El Houda , Danckaert Anne , Fernandes Julien , Roux Pascal , Charrueau Christine , Lachagès Anne-Marie , Charlotte Frédéric , Brocheriou Isabelle , Clément Karine , Aron-Wisnewsky Judith , Foufelle Fabienne , Ratziu Vlad , Hainque Bernard , Bonnefont-Rousselot Dominique , Bigey Pascal , Escriou Virginie
Published: March 2020

Hepatic fibrosis is a major consequence of chronic liver disease such as non-alcoholic steatohepatitis which is undergoing a dramatic evolution given the obesity progression worldwide, and has no treatment to date. Hepatic stellate cells (HSCs) play a key role in the fibrosis process, because in chronic liver damage, they transdifferentiate from a “quiescent” to an “activated” phenotype responsible for most the collagen deposition in liver tissue. Here, using a diet-induced liver fibrosis murine model (choline-deficient amino acid-defined, high fat diet), we characterized a specific population of HSCs organized as clusters presenting simultaneously hypertrophy of retinoid droplets, quiescent and activated HSC markers. We showed that hypertrophied HSCs co-localized with fibrosis areas in space and time. Importantly, we reported the existence of this phenotype and its association with collagen deposition in three other mouse fibrosis models, including CCl4-induced fibrosis model. Moreover, we have also shown its relevance in human liver fibrosis associated with different etiologies (obesity, non-alcoholic steatohepatitis, viral hepatitis C and alcoholism). In particular, we have demonstrated a significant positive correlation between the stage of liver fibrosis and HSC hypertrophy in a cohort of obese patients with hepatic fibrosis. These results lead us to conclude that hypertrophied HSCs are closely associated with hepatic fibrosis in a metabolic disease context and may represent a new marker of metabolic liver disease progression.
National Institutes of Health Stroke Scale (NIHSS) on admission predicts acute symptomatic seizure risk in ischemic stroke: a population-based study involving 135,117 cases
Authors: Zöllner Johann Philipp , Misselwitz Björn , Kaps Manfred , Stein Marco , Konczalla Jürgen , Roth Christian , Krakow Karsten , Steinmetz Helmuth , Rosenow Felix , Strzelczyk Adam
Published: March 2020

The National Institutes of Health Stroke Scale (NIHSS) score is the most frequently used score worldwide for assessing the clinical severity of a stroke. Prior research suggested an association between acute symptomatic seizures after stroke and poorer outcome. We determined the frequency of acute seizures after ischemic stroke in a large population-based registry in a central European region between 2004 and 2016 and identified risk factors for acute seizures in univariate and multivariate analyses. Additionally, we determined the influence of seizures on morbidity and mortality in a matched case–control design. Our analysis of 135,117 cases demonstrated a seizure frequency of 1.3%. Seizure risk was 0.6% with an NIHSS score at admission <3 points and increased up to 7.0% with >31 score points. Seizure risk was significantly higher in the presence of acute non-neurological infections (odds ratio: 3.4; 95% confidence interval: 2.8–4.1). A lower premorbid functional level also significantly increased seizure risk (OR: 1.7; 95%CI: 1.4–2.0). Mortality in patients with acute symptomatic seizures was almost doubled when compared to controls matched for age, gender, and stroke severity. Acute symptomatic seizures increase morbidity and mortality in ischemic stroke. Their odds increase with a higher NIHSS score at admission.
Toward Low-Cost All-Organic and Biodegradable Li-Ion Batteries
Authors: Delaporte N. , Lajoie G. , Collin-Martin S. , Zaghib K.
Published: March 2020

This work presents an alternative method for fabricating Li-ion electrodes in which the use of aluminum/copper current collectors and expensive binders is avoided. Low-cost natural cellulose fibers with a 2-mm length are employed as binder and support for the electrode. The objective of this method is to eliminate the use of heavy and inactive current collector foils as substrates and to replace conventional costly binders with cellulose fibers. Moreover, no harmful solvents, such as N-methylpyrrolidone, are employed for film fabrication. Water-soluble carbons are also utilized to reduce the preparation time and to achieve a better repartition of carbon in the electrode, thus improving the electrochemical performance. Flexible and resistant LiFePO4(LFP), Li4Ti5O12(LTO), organic 3,4,9,10-perylenetetracarboxylic dianhydride (PTCDA), and graphite electrodes are obtained with active mass loadings similar to those obtained by the current casting method. The initial discharge capacity of approximately 130 mAh·g−1at 2 C is obtained for an LFP/LTO paper battery with an approximately 91.6% capacity retention after 1000 cycles. An all-organic prelithiated PTCDA/graphite cell without a transition metal is prepared and electrochemically tested. It is one of the first self-standing batteries that is composed of organic redox active molecules and biodegradable components reported in literature.
Relating Natural Language Aptitude to Individual Differences in Learning Programming Languages
Authors: Prat Chantel S. , Madhyastha Tara M. , Mottarella Malayka J. , Kuo Chu-Hsuan
Published: March 2020

This experiment employed an individual differences approach to test the hypothesis that learning modern programming languages resembles second “natural” language learning in adulthood. Behavioral and neural (resting-state EEG) indices of language aptitude were used along with numeracy and fluid cognitive measures (e.g., fluid reasoning, working memory, inhibitory control) as predictors. Rate of learning, programming accuracy, and post-test declarative knowledge were used as outcome measures in 36 individuals who participated in ten 45-minute Python training sessions. The resulting models explained 50–72% of the variance in learning outcomes, with language aptitude measures explaining significant variance in each outcome even when the other factors competed for variance. Across outcome variables, fluid reasoning and working-memory capacity explained 34% of the variance, followed by language aptitude (17%), resting-state EEG power in beta and low-gamma bands (10%), and numeracy (2%). These results provide a novel framework for understanding programming aptitude, suggesting that the importance of numeracy may be overestimated in modern programming education environments.
A model study of terraced riverbeds as novel ecosystems
Authors: Yizhaq Hezi , Shachak Moshe , Meron Ehud
Published: March 2020

Riverbed terracing has been introduced in ancient times to retain water and soil, to reduce hydrological connectivity and erosion and to increase primary and secondary productivity of agro-ecological systems. These presently abandoned human-made landscapes have become novel ecosystems and a potential source of ecosystem services to humans in drylands. We use a mathematical-modeling approach to study factors that regulate terraced riverbeds and affect community and ecosystem attributes such as productivity, functional diversity and resilience to droughts. We introduce a model that captures the relationships between rainfall pattern, runoff coupling between adjacent terraces, and vegetation growth, taking into account competition for water and light. We found that a large number of weak rainfall events results in lower total biomass and functional diversity across the terraced riverbed compared with a few strong rainfall events. We further analyzed the filtering of species traits from pools of functional groups that make different tradeoffs between investment in above-ground biomass to capture canopy resources and investment in below-ground biomass to capture soil resources. Pools characterized by concave tradeoffs give rise to higher functional diversity, lower biomass production and lower resilience to droughts, as compared with convex pools. New empirical studies are needed to test these model predictions.
Substrate Specific Inhibitor Designed against the Immunomodulator GMF-beta Reversed the Experimental Autoimmune Encephalomyelitis
Authors: Vattathara Jane Jose , Prakash Ohm , Subhramanian Sunitha , Satheeshkumar Madathiparambil Kumaran , Xavier Tessy , Anil Meenakshi , Pillai Gopal S. , Anandakuttan Anandkumar , Radhakrishnan Sureshkumar , Sivanarayanan T. B. , AKK Unni , Mohan Chethampadi Gopi , Menon Krishnakumar N.
Published: March 2020

The concept of substrate inhibition to prevent its phosphorylation has potential in drug discovery and is envisioned to treat the autoimmune disorder multiple sclerosis (MS). Glia maturation factor-β (GMF-β) Ser83 phosphorylation by protein kinase A (PKA) is pivotal in the activation of GMF-β-p38MAPK-NFκB biochemical pathway towards proinflammatory response induction in experimental autoimmune encephalomyelitis (EAE). Using structure-based drug design, we identified the small molecule inhibitor 1-H-indazole-4yl methanol (GMFBI.1) that specifically blocked Ser83 phosphorylation site on GMF-β substrate. Usingin vitroandin vivotechniques, molecular mechanism of action of GMFBI.1’s direct interaction with GMF-β substrate and prevention of its Ser83 phosphorylation was established. GMFBI.1 down regulated p38MAPK phosphorylation and NFκB expression essential for proinflammatory response. Further, GMFBI.1 administration at peak of EAE reversed clinical symptoms, immunopathology, proinflammatory cytokine response and up regulated the anti-inflammatory cytokines. Present strategy of substrate inhibition against the key immunomodulatory target has immense therapeutic potential in MS.