bluuebunny/crossref_metadata_2025 huggingface dataset:
Symmetry reduction in a hyperpolarization-activated homotetrameric ion channel
Authors: Dickinson Miles Sasha , Gupta Meghna , Pourmal Sergei , Bi Maxine , Stroud Robert M.
Published: September 2021

Plants obtain nutrients from the soil via transmembrane transporters and channels in their root hairs, from which ions radially transport in towards the xylem for distribution across the plant body. We determined structures of the hyperpolarization-activated channel AKT1 from Arabidopsis thaliana, which mediates K+ uptake from the soil into plant roots. These structures of AtAKT1 embedded in lipid nanodiscs show that the channel undergoes a reduction of C4 to C2 symmetry, possibly to regulate its electrical activation.
Did the COVID-19 pandemic result in more family physicians stopping practice? Results from Ontario, Canada
Authors: Kiran Tara , Green Michael E. , Wu Fangyun C. , Kopp Alexander , Latifovic Lidija , Frymire Eliot , Glazier Richard H.
Published: September 2021

Purpose

To understand changes in family physician practice patterns and whether more family physicians stopped working during the COVID-19 pandemic compared to previous years.

Methods

We analyzed administrative data from Ontario, Canada two ways: cross-sectional and longitudinal. First, we identified the percentage and characteristics of all family physicians who had a minimum of 50 billing days in 2019 but no billings during the first six months of the pandemic. Second, for each year from 2010 to 2020, we calculated the percentage of physicians who billed for services in the first quarter of the calendar year but submitted no bills between April and September of the given year.

Results

We found 3.1% of physicians working in 2019 (N=385/12,247) reported no billings in the first six months of the pandemic. Compared with other family physicians, a higher portion were age 75 or older (13.0% vs. 3.4%, p<0.001), had fee-for-service reimbursement (38% vs 25%, p<0.001), and had a panel size under 500 patients (40% vs 25%, p<0.001). Between 2010 and 2019, an average of 1.6% of physicians who practiced in the first quarter had no billings in each of the second and third quarters of the calendar year compared to 3.0% in 2020 (p<0.001).

Conclusions

Approximately twice as many family physicians stopped work in Ontario, Canada during COVID-19 compared to previous years, but the absolute number was small and those who did had smaller patient panels. More research is needed to understand the impact on primary care attachment and access to care.
Crowding within synaptic junctions influence the degradation of adenoside nucleotides by CD39 and CD73 ectonucleotidases
Authors: Rahmaninejad Hadi , Pace Tom , Kekenes-Huskey Peter
Published: September 2021

1

Synapsed cells can communicate using exocytosed nucleotides like adenosine triphosphate (ATP). Ectonucleotidases localized to a synaptic junction degrade such nucleotides into metabolites like adenosine monophosphate (AMP) or adenosine, oftentimes in a sequential manner. CD39 and CD73 are a representative set of coupled ectonucleotidases, where CD39 first converts ATP and adenosine diphosphate (ADP) into AMP, after which the AMP product is dephosphorylated into adenosine by CD73. Hence, CD39/CD73 help shape cellular responses to extracellular ATP. In a previous study [1] we demonstrated that the rates of coupled CD39/CD73 activity within synapse-like junctions are strongly controlled by the enzymes’ co-localization, their surface charge densities, and the electrostatic potential of the surrounding cell membranes. In this study, we demonstrate that crowders within a synaptic junction, which can include globular proteins like cytokines and membrane-bound proteins, impact coupled CD39/CD73 electronucleotidase activity and in turn, the availability of intrasynapse ATP. Specifically, we simulated a spatially-explicit, reaction-diffusion model for the coupled conversion of ATP→AMP and AMP→adenosine in a model synaptic junction with crowders via the finite element method. Our modeling results suggest that the association rate for ATP to CD39 is strongly influenced by the density of intrasynaptic protein crowders, as increasing crowder density suppressed ATP association kinetics. Much of this suppression can be rationalized based on a loss of configurational entropy. The surface charges of crowders can further influence the association rate, with the surprising result that favorable crowder/nucleotide electrostatic interactions can yield CD39 association rates that are faster than crowder-free configurations. However, attractive crowder/nucleotide interactions decrease the rate and efficiency of adenosine production, which in turn increases the availability of ATP and AMP within the synapse relative to crowder-free configurations. These findings highlight how CD39/CD73 ectonucleotidase activity, electrostatics and crowding within synapses influence the availability of nucleotides for intercellular communication.
The Incubascope : a simple, compact and large field of view microscope for long-term imaging inside an incubator
Authors: Badon Amaury , Andrique Laetitia , Mombereau Amaël , Rivet Louis , Boyreau Adeline , Nassoy Pierre , Recher Gaëlle
Published: September 2021

Optical imaging has rapidly evolved in the last decades. Sophisticated microscopes allowing optical sectioning for 3D imaging or sub-diffraction resolution are available. Due to price and maintenance issues, these microscopes are often shared between users in facilities. Consequently, long term access is often prohibited and does not allow to monitor slowly evolving biological systems or to validate new models like organoids. Preliminary coarse long-term data that do not require acquisition of terabytes of high-resolution images are important as a first step. By contrast with expansive all-in-one commercialized stations, standard microscopes equipped with incubator stages offer a more cost-effective solution despite imperfect long run atmosphere and temperature control.

Here, we present the Incubascope, a custom-made compact microscope that fits into a table-top incubator. It is cheap and simple to implement, user-friendly and yet provides high imaging performances. The system has a field of view of 5.5 × 8 mm2, a 3 μm resolution, a 10 frames/second acquisition rate, and is controlled with a Python-based graphical interface. We exemplify the capabilities of the Incubascope on biological applications such as the hatching of Artemia salina eggs, the growth of the slime mold Physarum polycephalum and of encapsulated spheroids of mammalian cells.
Role of Disulfide Bonds in Membrane Partitioning of a Viral Peptide
Authors: Sikdar Samapan , Banerjee Manidipa , Vemparala Satyavani
Published: September 2021

The importance of disulfide bond in mediating viral peptide entry into host cells is well known. In the present work, we elucidate the role of disulfide (SS) bond in partitioning mechanism of membrane active Hepatitis A Virus-2B (HAV-2B) peptide, which harbours three cysteine residues promoting formation of multiple SS-bonded states. The inclusion of SS-bond not only results in a compact conformation but also induces distorted α-helical hairpin geometry in comparison to SS-free state, resulting in reduced hydrophobic exposure. Owing to this, the partitioning of HAV-2B peptide is completely or partly abolished. In a way, the disulfide bond regulates the partitioning of HAV-2B peptide, such that the membrane remodelling effects of this viral peptide are significantly reduced. The current findings may have potential implications in drug designing, targeting the HAV-2B protein by promoting disulfide bond formation within its membrane active region.
Beet taproot plasma membrane sugar transport revisited
Authors: Reyer Antonella , Bazihizina Nadia , Jaślan Justyna , Scherzer Sönke , Schäfer Nadine , Jaślan Dawid , Becker Dirk , Müller Thomas D. , Pommerrenig Benjamin , Neuhaus H. Ekkehard , Marten Irene , Hedrich Rainer
Published: September 2021

Sugar beet (Beta vulgaris) is the major sugar-producing crop in Europe and Northern America, as the taproot stores sucrose at a concentration of around 20%. Genome sequence analysis together with biochemical and electrophysiological approaches led to the identification and characterization of the TST sucrose transporter driving vacuolar sugar accumulation in the taproot. However, the sugar transporters mediating sucrose uptake across the plasma membrane of taproot parenchyma cells remained unknown. As with glucose, sucrose stimulation of taproot parenchyma cells caused inward proton fluxes and plasma membrane depolarization, indicating a sugar/proton symport mechanism. To decipher the nature of the corresponding proton-driven sugar transporters, we performed transcriptomic taproot profiling and identified the cold-induced PMT5a and STP13 transporters. When expressed inXenopus laevisoocytes, BvPMT5a was characterized as a voltage- and H+-driven low-affinity glucose transporter, which does not transport sucrose. In contrast, BvSTP13 operated as a high-affinity H+/sugar symporter, transporting glucose better than sucrose, and being more cold-tolerant than BvPMT5a. Modeling of the BvSTP13 structure with bound mono- and disaccharides suggests plasticity of the binding cleft to accommodate the different saccharides. The identification of BvPMT5a and BvSTP13 as taproot sugar transporters could improve breeding of sugar beet to provide a sustainable energy crop.

Significance Statement

In vivoelectrophysiological studies with sugar beet taproots provide clear evidence for proton-coupled glucose and sucrose uptake into taproot parenchyma cells. In search for the molecular entities, the taproot-expressed BvPMT5a and BvSTP13 carriers were studied in detail, because they mediate proton-driven import of glucose and sucrose and thus provide proper candidates for sugar beet plasma membrane sugar-proton cotransporters.
Cryptococcus neoformans releases proteins during intracellular residence that affect the outcome of the fungal-macrophage interaction
Authors: Jung Eric H. , Park Yoon-Dong , Dragotakes Quigly , Ramirez Lia Sanchez , Smith Daniel Q. , Dziedzic Amanda , Williamson Peter R. , Jedlicka Anne , Casadevall Arturo , Coelho Carolina
Published: September 2021

Cryptococcus neoformans is a facultative intracellular pathogen that can replicate and disseminate in mammalian macrophages. In this study, we analyzed fungal proteins identified in murine macrophage-like cells after infection with C. neoformans. To accomplish this, we developed a protocol to identify proteins released from cryptococcal cells inside macrophage-like cells; we identified 127 proteins of fungal origin in infected macrophage-like cells. Among the proteins identified was urease, a known virulence factor, and others such as transaldolase and phospholipase D, which have catalytic activities that could contribute to virulence. This method provides a straightforward methodology to study host-pathogen interactions. We chose to study further Yor1, a relatively uncharacterized protein belonging to the large family of ATP binding cassette transporter (ABC transporters). These transporters belong to a large and ancient protein family found in all extant phyla. While ABC transporters have an enormous diversity of functions across varied species, in pathogenic fungi they are better studied as drug efflux pumps. Analysis of C. neoformans yor1Δ strains revealed defects in non-lytic exocytosis and capsule size, when compared to wild-type strains. We detected no difference in growth rates, cell body size and vesicle secretion. Our results indicate that C. neoformans releases a large suite of proteins during macrophage infection, some of which can modulate fungal virulence and are likely to affect the fungal-macrophage interaction.
High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning
Authors: Getz Angela M. , Ducros Mathieu , Breillat Christelle , Lampin-Saint-Amaux Aurélie , Daburon Sophie , François Urielle , Nowacka Agata , Fernández-Monreal Mónica , Hosy Eric , Lanore Frédéric , Zieger Hanna , Sainlos Matthieu , Humeau Yann , Choquet Daniel
Published: September 2021

Regulation of synaptic neurotransmitter receptor content is a fundamental mechanism for tuning synaptic efficacy during experience-dependent plasticity and behavioral adaptation. However, experimental approaches to track and modify receptor movements in integrated experimental systems are limited. Exploiting AMPA-type glutamate receptors (AMPAR) as a model, we generated a knock-in mouse expressing the biotin acceptor peptide (AP) tag on the GluA2 extracellular N-terminus. Cell-specific introduction of biotin ligase allows the use of monovalent or tetravalent avidin variants to respectively monitor or manipulate the surface mobility of endogenous AMPAR containing biotinylated AP-GluA2 in neuronal subsets. AMPAR immobilization precluded the expression of long-term potentiation and formation of contextual fear memory, allowing for target-specific control of the expression of synaptic plasticity and animal behavior. The AP tag knock-in model offers unprecedented access to resolve and control the spatiotemporal dynamics of endogenous receptors, and opens new avenues to study the molecular mechanisms of synaptic plasticity and learning.
Over-expression of the brassinosteroid gene TaDWF4 increases wheat productivity under low and sufficient nitrogen through enhanced carbon assimilation
Authors: Milner Matthew J. , Swarbreck Stéphanie M. , Craze Melanie , Bowden Sarah , Griffiths Howard , Bentley Alison R. , Wallington Emma J.
Published: September 2021

There is a strong pressure to reduce nitrogen (N) fertiliser inputs while maintaining or increasing current cereal crop yields. Brassinosteroids, (BR), are a group of phytohormones essential for plant growth and development, that have been demonstrated to regulate several agronomic traits. DWF4 encodes a cytochrome P450 that catalyses a rate-limiting step in BR synthesis. We show that overexpression of the dominant shoot expressed homoeologue TaDWF4-B in wheat can increase plant productivity by up to 105% under a range of N levels on marginal soils, resulting in increased N use efficiency (NUE). We show that a two to four-fold increase in TaDWF4 transcript levels enhances the responsiveness of genes regulated by N. The productivity increases seen were primarily due to the maintenance of photosystem II operating efficiency and carbon assimilation in plants when grown under limiting N conditions and not an overall increase in photosynthesis capacity. The increased biomass production and yield per plant in TaDWF4 OE lines could be linked to modified carbon partitioning and changes in expression pattern of the growth regulator Target Of Rapamycin, offering a route towards breeding for sustained yield and lower N inputs.
miR2105 regulates ABA biosynthesis via OsbZIP86-OsNCED3module to contribute to drought tolerance in rice
Authors: Gao Weiwei , Li Mingkang , Yang Songguang , Gao Chunzhi , Su Yan , Zeng Xuan , Jiao Zhengli , Xu Weijuan , Zhang Mingyong , Xia Kuaifei
Published: September 2021

Induced abscisic acid (ABA) biosynthesis plays an important role in plant tolerance to abiotic stresses, including drought, cold and salinity. However, regulation pathway of the ABA biosynthesis in response to stresses is unclear. Here, we identified a rice miRNA, osa-miR2105 (miR2105), which plays a crucial role in ABA biosynthesis under drought stress. Analysis of expression, transgenic rice and cleavage site showed thatOsbZIP86is a target gene of miR2105. Subcellular localization and luciferase activity assays showed that OsbZIP86 is a nuclear transcription factor.In vivoandin vitroanalyses showed that OsbZIP86 directly binds to the promoter ofOsNCED3, and interacts with OsSAPK10, resulting in enhanced-expression ofOsNCED3. Transgenic rice plants with knock-down of miR2105 or overexpression ofOsbZIP86showed higher ABA content, more tolerance to drought, a lower rate of water loss, more stomatal closure than wild type rice ZH11 under drought stress. These rice plants showed no penalty with respect to agronomic traits under normal conditions. By contrast, transgenic rice plants with miR2105 overexpression,OsbZIP86downregulation, orOsbZIP86knockout displayed less tolerance to drought stress and other phenotypes. Collectively, our results show that a regulatory network of ‘miR2105-OsSAPK10/OsbZIP86-OsNCED3’ control ABA biosynthesis in response to drought stress.

One-sentence summary

‘miR2105-OsbZIP86-OsNCED3’ module plays crucial role in mediating ABA biosynthesis to contribute to drought tolerance with no penalty with respect to agronomic traits under normal conditions.
Investigation of the impact of stool collection methods on the metabolomics analysis/profiles of infant fecal samples
Authors: Homann Chiara-Maria , Dizzell Sara , Azab Sandi M. , Hutton Eileen K. , Morrison Katherine M. , Stearns Jennifer C.
Published: September 2021

Metabolomic studies are important to understand microbial metabolism and interaction between the host and the gut microbiome. Although there have been efforts to standardize sample processing in metabolomic studies, infant samples are mostly disregarded. In birth cohort studies, the use of diaper liners is prevalent and its impact on fecal metabolic profile remains untested. In this study, we compared metabolite profiles of fecal samples collected as solid stool and those collected from stool saturated liner. One infant’s stool sample was collected in triplicate for solid stool and stool saturated liner. Comprehensive metabolomics analysis of the fecal samples was performed using NMR, UPLC and DI-MS. The total number, identities and concentrations of the metabolites were determined and compared between stool sample collection methods (stool vs. liner). The number and identity of metabolites did not differ between collection methods for NMR and DI-MS when excluding metabolites with a coefficient of variation (CV) > 40%. NMR analysis demonstrated lowest bias between collection methods, and lowest technical precision between triplicates of the same method followed by DI-MS then UPLC. Concentrations of many metabolites from stool and stool saturated liner differed significantly as revealed by Bland-Altman plots and t-tests. Overall, a mean bias of 10.2% in the Bland-Altman analysis was acceptable for some metabolites confirming mutual agreement but not for others with a wide range of bias (−97-117%). Consequently, stool and stool-saturated liner could be used interchangeably only for some select metabolite classes e.g. amino acids. Differences between the metabolomic profiles of solid stool samples and stool saturated liner samples for some important molecules e.g., ethanol, fumarate, short chain fatty acids and bile acids, indicate the need for standardization in stool collection method for metabolomic studies performed in infants.
Selective Microautophagy of Proteasomes is Initiated by ESCRT-0 and Is Promoted by Proteasome Ubiquitylation
Authors: Li Jianhui , Hochstrasser Mark
Published: September 2021

The proteasome is central to proteolysis by the ubiquitin-proteasome system under normal growth conditions but is itself degraded through macroautophagy under nutrient stress. A recently described AMPK (AMP-activated protein kinase)-regulated ESCRT (endosomal sorting complex required for transport)-dependent microautophagy pathway also regulates proteasome trafficking and degradation in low glucose conditions in yeast. Aberrant proteasomes are more prone to microautophagy, suggesting the ESCRT system fine-tunes proteasome quality control under low glucose stress. Here we uncover additional features of the selective microautophagy of proteasomes. Genetic or pharmacological induction of aberrant proteasomes is associated with increased mono- or oligo-ubiquitylation of proteasome components, which appear to be recognized by ESCRT-0. AMPK controls this pathway in part by regulating the trafficking of ESCRT-0 to the vacuole surface, which also leads to degradation of the Vps27 subunit of ESCRT-0. The Rsp5 ubiquitin ligase contributes to proteasome subunit ubiquitylation, and multiple ubiquitin-binding elements in Vps27 are involved in their recognition. We propose that ESCRT-0 at the vacuole surface recognizes ubiquitylated proteasomes and initiates their microautophagic elimination during glucose depletion.

Saummary statement

ESCRT-0 selectively targets aberrant proteasomes for microautophagy by recognition of proteasome ubiquitylation status to fine-tune proteasome quality control under low glucose conditions.
The nuclear transport factor CSE1 drives macronuclear volume increase and macronuclear node coalescence inStentor coeruleus
Authors: McGillivary Rebecca M. , Sood Pranidhi , Hammar Katherine , Marshall Wallace F.
Published: September 2021

The giant ciliate,Stentor coeruleus, provides a unique opportunity to study nuclear shape because its macronucleus undergoes a rapid, dramatic, and developmentally regulated shape change. During a 2 hour time period within cell division and regeneration, the 400 um long moniliform macronucleus coalesces into a single mass, elongates into a vermiform shape, and then renodulates, returning to its original beads-on-a-string morphology.1Previous work from the 1960’s - 1980’s demonstrated that the macronuclear shape change is a highly regulated part of cell division and regeneration2,3, but the molecular pathways driving these changes are unknown. With the recent availability of a sequencedStentorgenome, a transcriptome during regeneration, and molecular tools like RNAi4–6, it is now possible to investigate the molecular mechanisms that drive macronuclear shape change. We found that the volume of the macronucleus increases during coalescence, suggesting an inflation-based mechanism. When the nuclear transport factor, CSE1, is knocked down by RNAi, the shape and volume changes of the macronucleus are attenuated, and nuclear morphology is altered. CSE1 protein undergoes a dynamic relocalization correlated with nuclear shape changes, being mainly cytoplasmic prior to nuclear coalescence, and accumulating inside the macronucleus during coalescence. At the end of regeneration, CSE1 is degraded during the period when the macronucleus returns to its pre-coalescence volume. We propose a model in which nuclear transport via CSE1 increases the volume of the macronucleus, thereby decreasing the surface to volume ratio and driving coalescence of the many nodes into a single mass.
Design of Peptide Vaccine for COVID19: CD8+ and CD4+ T cell epitopes from SARS-CoV-2 open-reading-frame protein variants
Authors: Parn Simone , Jabbour Gabriel , Nguyenkhoa Vincent , Dakshanamurthy Sivanesan
Published: September 2021

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, has challenged public health at an unprecedented scale which has led to a dramatic loss of human life worldwide. To design a protective vaccine against SARS-CoV-2, it is necessary to understand which SARS-CoV-2 specific epitopes can elicit a T cell response and provide protection across a broad population. In this study, PLpro and RdRp, two immunogenic non-structural proteins from an immunodominant gene region ORF1ab, as well as ORF3a and ORF9b are identified as potential vaccine targets against SARS-CoV-2. To select top epitopes for vaccine design, we used various clinical properties, such as antigenicity, allergenicity, toxicity and IFN-y secretion. The analysis of CD8 and CD4 T cell epitopes revealed multiple potential vaccine constructs that cover a high percentage of the world population. We identified 8 immunogenic, antigenic, non-allergenic, non-toxic, stable and IFN-y inducing CD8 proteins for nsp3, 4 for nsp12, 11 for ORF3a and 3 for ORF9b that are common across four lineages of variants of concern – B.1.1.7, P.1, B.1.351 and B.1.617.2, which protect 98.12%, 87.08%, 96.07% and 63.8% of the world population, respectively. We also identified variant specific T cell epitopes that could be useful in targeting each variant strain separately. Including the prediction of mouse MHC affinity towards our top CD8 epitopes, our study revealed a total of 3 immunogenic, antigenic, non-allergenic, non-toxic, stable and IFN-y inducing CD8 epitopes overlapping with 6 antigenic, non-allergenic, non-toxic, stable and IFN-y inducing CD4 epitopes across all four variants of concern which can effectively be utilized in pre-clinical studies. The landscape of SARS-CoV-2 T cell epitopes that we identified can help lead SARS-CoV-2 vaccine development as well as epitope-based peptide vaccine research in the future.
Association of RACK1 protein with ribosomes in Plasmodium falciparum
Authors: Erath Jessey , Djuranovic Sergej
Published: September 2021

The receptor for activated C-kinase 1 (RACK1), a highly conserved eukaryotic protein, is known to have many, varying biological roles and functions. Previous work has established RACK1 as a ribosomal protein, with defined regions important for binding ribosomes in both human and yeast cells. In Plasmodium falciparum, RACK1 has been shown to be required for parasite growth, however conflicting evidence has been presented about the RACK1 ribosome binding and its role in mRNA translation. Given the importance of RACK1 as a regulatory component of mRNA translation and ribosome quality control, the case could be made in parasites for either of the binary options: bound or unbound to the ribosome. Here we used bioinformatics and transcription analyses to describe P. falciparum RACK1 protein. Based on homology modeling and structural analyses, we generated a model of P. falciparum RACK1 protein. We created and explored mutant and chimeric human and P. falciparum RACK1 proteins binding properties to the human and P. falciparum ribosome. Wild type, chimeric and mutant RACK1 proteins suggest different binding characteristics for P. falciparum and human RACK1 proteins and different regions being involved in their ribosome association. The ribosomal binding of RACK1 variants in human and parasite cells shown here demonstrates that although RACK1 proteins have highly conserved sequences and structures across species, ribosomal binding is affected by species specific alterations to the protein. In conclusion, we show that in the case of P. falciparum, contrary to the structural data, RACK1 is found to be bound to ribosomes and in actively translating polysomes of P. falciparum cells.
Visuospatial information foraging describes search behavior in learning latent environmental features
Authors: Barack David L , Bakkour Akram , Shohamy Daphna , Salzman C Daniel
Published: September 2021

In the real world, making sequences of decisions to achieve goals often depends upon the ability to learn aspects of the environment that are not directly perceptible. Learning these so-called latent features requires seeking information about them, a process distinct from learning about near-term reward contingencies. Prior efforts to study latent feature learning often use single decisions, use few features, and fail to distinguish between reward-seeking and informationseeking. To overcome this, we designed a task in which humans and monkeys made a series of choices to search for shapes hidden on a grid. Reward and information outcomes from uncovering parts of shapes were not perfectly correlated and their effects could be disentangled. Members of both species adeptly learned the shapes and preferred to select informative tiles earlier in trials than rewarding ones, searching a part of the grid until their outcomes dropped below the average information outcome–a pattern consistent with foraging behavior. In addition, how quickly humans learned the shapes was predicted by how well their choice sequences matched the foraging pattern. This adaptive search for information may underlie the ability in humans and monkeys to learn latent features to support goal-directed behavior in the long run.
Epithelial Plasticity and Innate Immune Activation Promote Lung Tissue Remodeling following Respiratory Viral Infection
Authors: Beppu Andrew K. , Zhao Juanjuan , Yao Changfu , Carraro Gianni , Israely Edo , Coelho Anna Lucia , Hogaboam Cory M , Parks William C. , Kolls Jay K. , Stripp Barry R.
Published: September 2021

Epithelial plasticity has been suggested in lungs of mice following genetic depletion of stem cells but is of unknown physiological relevance. Viral infection and chronic lung disease share similar pathological features of stem cell loss in alveoli, basal cell (BC) hyperplasia in small airways, and innate immune activation, that contribute to epithelial remodeling and loss of lung function. We show that a novel subset of distal airway secretory cells, intralobar serous (IS) cells, are activated to assume BC fates following influenza virus infection. Injury-induced hyperplastic BC (hBC) differ from pre-existing BC by high expression of IL-22Ra1 and undergo IL-22-dependent expansion for colonization of injured alveoli. Resolution of virus-elicited inflammation resulted in BC>IS re-differentiation in repopulated alveoli, and increased local expression of antimicrobial factors, but failed to replace normal alveolar epithelium. Epithelial plasticity therefore protects against mortality from acute respiratory viral infection but results in distal lung remodeling and loss of lung function.
Applying systematic bibliometric methods to track a journal’s impact and review its knowledge contribution
Authors: Anand Amitabh , Tripathi Ritu , Karumathil Anjana , Kalra Tanvika
Published: September 2021

Purpose

Advanced bibliometric methods have emerged as key tools in mapping the history and trends of a discipline. This paper aims to demonstrate on applying various bibliometric methods to track a journal’s impact and review its knowledge contribution. In doing so, the authors take the case ofIIMB Management Review (IMR)journal focused on management discipline, in consideration of its 10 years of publication presence.

Design/methodology/approach

Using bibliometric and Scopus metric methods, the authors map and analyze the productivity of IMR Journal and map its knowledge contributions.

Findings

The authors identify the IMR journal’s impact, its growth, the most prolific authors/affiliations, key research hotspots, cross-country collaboration and emerging trends over the past decade.

Originality/value

A 10-year longitudinal review helps the target group identify the main themes. It also provides key empirical insights to the journal editorial board and library managers for future planning and growth of the journal.
Predictors of e-publishing adoption in environments of uncertainty
Authors: Ifeduba Emmanuel Chukwunonye
Published: September 2021

Purpose

Many developing environments are characterised by uncertainties and research on how these uncertainties impact development in different industries is on-going. However, there is hardly any empirical examination of how this phenomenon impacts innovation adoption in the publishing industry, notwithstanding that the education industry largely depends on publishing. This study aims to interrogate this phenomenon with a view to describing clearly the factors that influence e-publishing innovation adoption in environments of uncertainty.

Design/methodology/approach

E-publishing data were collected from 79 websites whereas 109 firms filled out a questionnaire both online and offline. Four interviews were conducted and data were analysed using the SPSS to compute frequencies, percentages and correlates of digital publishing innovation adoption.

Findings

Book piracy and curriculum uncertainty were found to play greater influential roles in the adoption of e-publishing; and though they both correlated positively with e-publishing adoption, only book piracy has a significant predictive value in the adoption of e-publishing.

Originality/value

The results of this study shed light on the predictors of digital publishing adoption and should help interested publishers and scholars in environments of uncertainty to understand why efforts should be intensified to pursue copyright protection and enforcement.
Entropy generation analysis on unsteady flow of Maxwell nanofluid over the stretched wedge with Cattaneo-Christov double diffusion
Authors: Bai Yu , Fang Huiling , Zhang Yan
Published: September 2021

Purpose

This paper aims to present the effect of entropy generation on the unsteady flow of upper-convected Maxwell nanofluid past a wedge embedded in a porous medium in view of buoyancy force. Cattaneo-Christov double diffusion theory simulates the processes of energy phenomenon and mass transfer. Meanwhile, Brownian motion, thermophoresis and convective boundary conditions are discussed to further visualize the heat and mass transfer properties.

Design/methodology/approach

Coupled ordinary differential equations are gained by appropriate similar transformations and these equations are manipulated by the Homotopy analysis method.

Findings

The result is viewed that velocity distribution is a diminishing function with boosting the value of unsteadiness parameter. Moreover, fluid friction irreversibility is dominant as the enlargement in Brinkman number. Then controlling the temperature and concentration difference parameters can effectively regulate entropy generation.

Originality/value

This paper aims to address the effect of entropy generation on unsteady flow, heat and mass transfer of upper-convected Maxwell nanofluid over a stretched wedge with Cattaneo-Christov double diffusion, which provides a theoretical basis for manufacturing production.
Clustering the countries for quantifying the status of Covid-19 through time series analysis
Authors: Erandathi Madurapperumage , Chung Wang William Yu , Hsieh Chih-Chia
Published: September 2021

Purpose

This study aims to use financial stability and health facilities of countries, to cluster them for making a more consensus environment for manifesting the status of Covid-19 in a justifiable manner. The scarcity of the categorisation of the countries of the world in a common platform, and the requirement of manifesting the pandemic status such as Covid-19 in a justifiable manner create the demanding requirement. This study mainly focusses on assisting to generate a liable manifesto to criticise the span of viral infection of the severe acute respiratory syndrome coronavirus-2 over the globe.

Design/methodology/approach

Data for this study has been gathered from official websites of the World Bank, and the world in data. The Louvain clustering method has been used to cluster the countries based on their financial strength and health facilities. The resulted clusters are visualised using Silhouette plots. The anomalies of the clusters had been used to quantify the pandemic situation. The status of Covid-19 has been manifested with the time series analysis through python programming.

Findings

The countries of the world have been clustered into seven, where developed countries divided into three clusters and the countries with transition economies and developing clustered together into four clusters. The time series analysis of recognised anomalies of the clusters assist to monitor the government responses and analyse the efficiency of used safety measures against the pandemic.

Originality/value

This study’s resulted clusters are highly valuable as a division of countries of the whole world for evaluating the health systems and for the regional levels. Further, the results of time series analysis are beneficial in monitoring the government responses and analysing the efficiency of used safety measures against the pandemic.
Determinants of instant messaging use for communication with hotels: a policy-capturing study
Authors: Lei Sut Ieng , Kirillova Ksenia , Wang Dan , Xiao Chuan
Published: September 2021

Purpose

Mobile instant messaging (IM) has been increasingly adopted by hotels to communicate with customers. This study aims to explore communication between hotels and customers and identifies the factors that affect hotel customers’ intention to use mobile IM to communicate with hotels.

Design/methodology/approach

A two-stage exploratory sequential mixed-method design, which combines semi-structured interview and policy-capturing method was applied.

Findings

The findings indicate that customers are more likely to use mobile IM to communicate with hotels for non-urgent matters; before and after a stay; and if customers are accustomed to using mobile IM for work and non-work purposes in daily life.

Research limitations/implications

This study goes beyond traditional theories to capturing communication-related factors that affect customers’ IM use in a hotel context.

Practical implications

The findings indicate why hotel managers should avoid relying on IM as the dominant communication channel.

Originality/value

This is among one of the first studies that explore customers’ communication needs and communication media choice in hotels.
A systematic review of work-life integration and role of flexible work arrangements
Authors: Kumar Sourabh , Sarkar Sankersan , Chahar Bhawna
Published: September 2021

Purpose

The growing demands of work and life have shifted the concept of work-life balance to work-life integration (WLI). The success of integration depends upon the flexibility to perform the duties. This paper aims to explore the factors that affect WLI and the role of flexible work arrangements (FWAs) in the process of WLI.

Design/methodology/approach

Systematic literature review was used to explore the concept of WLI and FWAs. A bibliometric analysis was carried out with Bibexcel and VoSviewer.

Findings

This paper explained the organizational and personal factors that create the demand for WLI. The FWAs, perceived flexibility, technology and self-efficacy have important roles in WLI. The result of WLI can be enrichment or strain, depends upon how effectively the work-life domains are integrated.

Originality/value

This paper explores the work-life from both personal and organizational views. The findings of this paper will be useful to design the organizational policies and work arrangements that match the requirements of employees and organizations. This paper helps to develop the future research agenda of investigating the relations of WLI to performance, organizational policies and personal factors.
The ageing prisoner population: demographic shifts in Australia and implications for the economic and social costs of health care
Authors: Ginnivan Natasha Ann , Chomik Rafal , Hwang Ye In (Jane) , Piggott John , Butler Tony , Withall Adrienne
Published: September 2021

Purpose

The Australian prisoner population has experienced a dramatic increase in the number of older inmates over the past decade, consistent with the greying of the prisoner population that is being observed worldwide. Reviews suggest the need for further evidence and practice outside of the USA. This paper aims to review and discuss the cost and social implications of the rising health-care needs of this population in Australia.

Design/methodology/approach

A review of international research and policies is presented, as well as the results of basic economic modelling relating to the expected rise in health-care costs of the ageing prisoner population in Australia.

Findings

Taking into consideration the continued rise in incarceration rates, the calculations show that the health costs of prisoners could increase by anywhere between 17% and 90% depending on whether the increase of older prisoners continues as it has in the past decade. These trends are likely to continue over the next decade and will result in higher health costs of prisons under a number of different imprisonment scenarios. Policy responses in Australia have been slow so far, with most initiatives being undertaken in the USA with promising results.

Practical implications

The authors suggest that in the absence of a coordinated policy response, covering a range of interventions, costs will continue to increase, particularly as this population continues to age more rapidly than the general population due to an accumulation of risk factors. Well-conceived interventions would be a worthwhile investment from both financial and social perspectives.

Originality/value

To the best of the authors’ knowledge, this is the first commentary to acknowledge this rising public health issue and to both review and model its implications for the future.
Design framework for a lean warehouse – a case study-based approach
Authors: Raghuram P. , Arjunan Mahesh Kumar
Published: September 2021

Purpose

This purpose of this study is to develop a simple framework for designing a warehouse incorporating lean principles. Multiple objectives like resource planning, material handling, storage, inventory management, including internal and external logistics, are considered.

Design/methodology/approach

A design procedure to incorporate lean principles for designing a warehouse for a complex multi-model production line has been proposed. The preferred standards and factors affecting warehouse design, the inputs and outputs of process flow characteristics, are incorporated into the design. Current and future state value stream mappings are drawn to bring out the challenges in the value flow.

Findings

The framework for designing a lean warehouse have been implemented and validated in a heavy machinery manufacturer. This framework will ease the work of the future lean-based warehouse designers to apply simple step-by-step processes to achieve the goal with the nearest accuracy. The steps followed can be summarized as defining the lean processes, making the lean process as the design base, collecting inputs like stock-keeping unit master, inventory and space details, and building the lean warehouse design with the step-by-step processes.

Practical implications

Practical tips on warehouse design have been explained focusing on the part volume, quantity handled, inventory and throughput. This will assist the practitioners in designing a lean warehouse and leading to an improved operational performance.

Originality/value

A simplified design procedure for designing a lean warehouse, along with a real-time case study has been enumerated in detail. Effective use of space and resources with lean tools and techniques lead to better storage and picking efficiency resulting in an overall reduction in cost.
Synthesis and use of TMP ester biolubricant derived from cottonseed oil in SI engine
Authors: Khan Muhammad Bilal , Zahid Rehan , Kazim Ali Hussain , Javed Khalid
Published: September 2021

Purpose

Depleting reserves of crude oils and their adverse environmental effects have shifted focus toward environment friendly and biobased lubricant base oils. Natural oils and fats act as good lubricants but they have low oxidation and thermal stability which makes them unsuitable for modern day uses. This paper aims to produce trimethylolpropane ester biolubricant from cottonseed oil and study the effects of its use in spark ignition (SI) engines.

Design/methodology/approach

In this work, cottonseed oil is converted to TMP lubricant by a two-step based catalyzed esterification. The lubricants thermophysical properties are then analyzed and a 20% blend with synthetic poly-alpha olefin is used in an spark ignition engine.

Findings

The produced lubricant has viscosity @100oC of 4.91 cSt, a viscosity index of 230 and a flash point of 202oC. When used as a 20% blend in a petrol engine, the rate of oil deterioration was reduced by 18%, however, the overall wear increased by 6.7%. However, this increase is offset by its improved environmental impacts.

Originality/value

In its current state, such a biolubricant can be used as an additive to most commercially available lubricants to improve oil deterioration characteristics and environmental impact. However, further work on improving biolubricant’s wear characteristics is needed for the complete replacement of mineral oil-based lubricants.
Co-exploitation (or co-exploration) vs go it alone? The role of alliance learning capability on firm performance
Authors: Solís-Molina Miguel , Hernández-Espallardo Miguel , Rodríguez-Orejuela Augusto
Published: September 2021

Purpose

This study aims to analyze the moderating role of a firm’s alliance learning capability. The aim is to investigate the comparative performance of developing exploitation (or exploration) activities in collaboration with others vs adopting a go-it-alone posture.

Design/methodology/approach

The authors compare high levels of co-exploitation (or co-exploration) that represent the collaboration stance vs low levels of co-exploitation (or co-exploration) that characterize the go-it-alone posture. Data were collected using a sample of 262 manufacturing firms that developed exploitation-based innovations and 239 exploration-based innovations. Regression models were used to test the hypotheses.

Findings

Empirical results suggest that the best performance is reached by firms that exploit or explore collaborating with others at high levels of alliance learning capability. In contrast, firms perform better by going alone in exploitation activities at low levels of alliance learning capability.

Practical implications

Firms may complement internal efforts of exploitation or exploration by co-developing knowledge with other organizations for higher performance. However, collaborating with others is not free of drawbacks, and, under certain circumstances, the go-it-alone strategy is more convenient.

Originality/value

This paper provides evidence of the role of a firm’s alliance learning capability in determining the differential performance of carrying on exploitation or exploration activities in collaboration with others vs adopting a go-it-alone stance. Thus, it offers an alternative perspective in the literature on organizational learning and innovation management, in contrast with the exploitation and exploration balanced perspective of ambidexterity, by explaining how alliance learning capability fosters firm performance combining exploitation or exploration at organizational and inter-organizational levels.
Trust in the academy: a conceptual framework for understanding trust on academic web profiles
Authors: Francke Helena
Published: September 2021

Purpose

Institutional and commercial web profiles that provide biobibliographic information about researchers are used for promotional purposes but also as information sources. In the latter case, the profiles' (re)presentations of researchers may be used to assess whether a researcher can be trusted. The article introduces a conceptual framework of how trust in researchers may be formed based on how the researchers' experiences and achievements are mobilized on the profiles to tell a multifaceted story of the “self.”

Design/methodology/approach

The framework is an analytical product which draws on theories of trust as well as on previous research focused on academic web profiles and on researchers' perceptions of trust and credibility. Two dimensions of trust are identified as central to the theoretical construction of trust, namely competence and trustworthiness.

Findings

The framework outlines features of profile content and narrative that may influence the assessment of the profile and of the researcher's competence and trustworthiness. The assessment is understood as shaped by the frames of interpretation available to a particular audience.

Originality/value

The framework addresses the lack of a trust perspective in previous research about academic web profiles. It provides an analysis of how potential trust in the researcher may be formed on the profiles. An innovative contribution is the acknowledgement of both qualitative and quantitative indicators of trustworthiness and competence, including the richness of the story told about the “self.”