bluuebunny/crossref_metadata_2025 huggingface dataset:
Transfection by Electroporation of Cancer and Primary Cells Using Nanosecond and Microsecond Electric Fields
Authors: Radzevičiūtė Eivina , Malyško-Ptašinskė Veronika , Novickij Jurij , Novickij Vitalij , Girkontaitė Irutė
Published: June 2022

Gene transfer into primary immune cells as well as into cell lines is essential for scientific and therapeutical applications. One of the methods used for gene transfer is electroporation (EP). EP is a method where a pulsed electric field (PEF) causes a highly transient permeability of the targeted cell membrane. In this work, we present the electrotransfection of CHO-K1, 4T1 cell lines, and primary murine DCs with detectable protein-encoding plasmids in the sub-microsecond range. Microsecond (µs)- and nanosecond (ns)-range pulsed electric field transfection protocols were used. The efficiency of electrotransfection was evaluated using green fluorescent protein (GFP)-encoding plasmids (4.7 kbp; p-EGFP-N1) and plasmids expressing a firefly luciferase and red fluorescent protein (tdTomato) (8.5 kbp; pcDNA3.1(+)/Luc2 = tdT)). It was shown that the used nsPEFs protocol (7 kV/cm × 300 ns × 100, 1 MHz) ensured a better transfection efficiency than µsPEFs (1.2 kV/cm × 100 µs × 8, 1 Hz). Plasmid size and concentration had a strong impact on the cell transfection efficiency too. We also showed that there were no significant differences in transfection efficiency between immature and mature DCs. Finally, the nsPEF protocols were successfully applied for the stable transfection of the CHO-K1 cell line with the linearized pcDNA3.1(+)/Luc2 = tdT plasmid. The results of the study are applicable in gene therapy and DNA vaccination studies for the derivation of optimal electrotransfection conditions.
The Effect of Pregnancy and Inflammatory Bowel Disease on the Pharmacokinetics of Drugs Related to Inflammatory Bowel Disease—A Systematic Literature Review
Authors: Wiersma Thomas K. , Visschedijk Marijn C. , de Boer Nanne K. , Lub-de Hooge Marjolijn N. , Prins Jelmer R. , Touw Daan J. , Mian Paola
Published: June 2022

Due to ethical and practical reasons, a knowledge gap exists on the pharmacokinetics (PK) of inflammatory bowel disease (IBD)-related drugs in pregnant women with IBD. Before evidence-based dosing can be proposed, insight into the PK has to be gained to optimize drug therapy for both mother and fetus. This systematic review aimed to describe the effect of pregnancy and IBD on the PK of drugs used for IBD. One aminosalicylate study, two thiopurine studies and twelve studies with biologicals were included. Most drugs within these groups presented data over multiple moments before, during and after pregnancy, except for mesalazine, ustekinumab and golimumab. The studies for mesalazine, ustekinumab and golimumab did not provide enough data to demonstrate an effect of pregnancy on concentration and PK parameters. Therefore, no evidence-based dosing advice was given. The 6-thioguanine nucleotide levels decreased during pregnancy to 61% compared to pre-pregnancy levels. The potentially toxic metabolite 6-methylmercaptopurine (6-MMP) increased to maximal 209% of the pre-pregnancy levels. Although the PK of the thiopurines changed throughout pregnancy, no evidence-based dosing advice was provided. One study suggested that caution should be exercised when the thiopurine dose is adjusted, due to shunting 6-MMP levels. For the biologicals, infliximab levels increased, adalimumab stayed relatively stable and vedolizumab levels tended to decrease during pregnancy. Although the PK of the biologicals changed throughout pregnancy, no evidence-based dosing advice for biologicals was provided. Other drugs retrieved from the literature search were mesalazine, ustekinumab and golimumab. We conclude that limited studies have been performed on PK parameters during pregnancy for drugs used in IBD. Therefore, more extensive research to determine the values of PK parameters is warranted. After gathering the PK data, evidence-based dosing regimens can be developed.
A Hybrid of Amodiaquine and Primaquine Linked by Gold(I) Is a Multistage Antimalarial Agent Targeting Heme Detoxification and Thiol Redox Homeostasis
Authors: De Souza Pereira Caroline , Quadros Helenita Costa , Aboagye Samuel Yaw , Fontinha Diana , D’Alessandro Sarah , Byrne Margaret Elizabeth , Gendrot Mathieu , Fonta Isabelle , Mosnier Joel , Moreira Diogo Rodrigo M. , Basilico Nicoletta , Williams David L. , Prudêncio Miguel , Pradines Bruno , Navarro Maribel
Published: June 2022

Hybrid-based drugs linked through a transition metal constitute an emerging concept for Plasmodium intervention. To advance the drug design concept and enhance the therapeutic potential of this class of drugs, we developed a novel hybrid composed of quinolinic ligands amodiaquine (AQ) and primaquine (PQ) linked by gold(I), named [AuAQPQ]PF6. This compound demonstrated potent and efficacious antiplasmodial activity against multiple stages of the Plasmodium life cycle. The source of this activity was thoroughly investigated by comparing parasite susceptibility to the hybrid’s components, the annotation of structure–activity relationships and studies of the mechanism of action. The activity of [AuAQPQ]PF6 for the parasite’s asexual blood stages was influenced by the presence of AQ, while its activity against gametocytes and pre-erythrocytic parasites was influenced by both quinolinic components. Moreover, the coordination of ligands to gold(I) was found to be essential for the enhancement of potency, as suggested by the observation that a combination of quinolinic ligands does not reproduce the antimalarial potency and efficacy as observed for the metallic hybrid. Our results indicate that this gold(I) hybrid compound presents a dual mechanism of action by inhibiting the beta-hematin formation and enzymatic activity of thioredoxin reductases. Overall, our findings support the potential of transition metals as a dual chemical linker and an antiplasmodial payload for the development of hybrid-based drugs.
All-Dielectric Structural Colors with Lithium Niobate Nanodisk Metasurface Resonators
Authors: Zhou Yuting , Wang Qingyu , Ji Zhiqiang , Zeng Pei
Published: June 2022

Lithium niobate (LN) is a promising optical material, its micro–nano structures have been applied to fields such as photonic crystals, nonlinear optics, optical waveguides, and so on. At present, lithium niobate structural colors are rarely studied. Although the nanograting structure was researched, it has such large full width at half-maximum (fwhm) that it cannot achieve red, green, or blue pixels or other high-saturation structural colors, thus, its color printing quality is poor. In this paper, we design and simulate lithium niobate nanodisk metasurface resonators (LNNDMRs), which are based on Mie magnetic dipole (MD) and electric dipole (ED) resonances. In addition, the resonators yield very narrow reflection peaks and high reflection efficiencies with over 80%, especially the reflection peaks of red, green, and blue pixels with fwhm around 11 nm, 9 nm, and 6 nm, respectively. Moreover, output colors of different array cells composed of single nanodisk in finite size are displayed, which provides a theoretical basis for their practical applications. Therefore, LNNDMRs pave the way for high-efficiency, compact photonic display devices based on lithium niobate.
Geometric Progression of Optical Vortices
Authors: Kotlyar Victor , Kovalev Alexey , Kozlova Elena , Savelyeva Alexandra , Stafeev Sergey
Published: June 2022

We study coaxial superpositions of Gaussian optical vortices described by a geometric progression. The topological charge (TC) is obtained for all variants of such superpositions. The TC can be either integer or half-integer in the initial plane. However, it always remains integer when the light field propagates in free space. In the general case, the geometric progression of optical vortices (GPOV) has three integer parameters and one real parameter, values which define its TC. The GPOV does not conserve its intensity structure during propagation in free space. However, the beam can have the intensity lobes whose number is equal to one of the family parameters. If the real GPOV parameter is equal to one, then all angular harmonics in the superposition are of the same energy. In this case, the TC of the superposition is equal to the order of the average angular harmonic in the progression. Thus, if the first angular harmonic in the progression has the TC of k and the last harmonic has the TC of n, then the TC of the entire superposition in the initial plane is equal to (n + k)/2, but the TC is equal to n during propagation. The experimental results on generating of the GPOVs by a spatial light modulator are in a good agreement with the simulation results.
SNR Enhancement of Direct Absorption Spectroscopy Utilizing an Improved Particle Swarm Algorithm
Authors: Zhang Lin , Li Yanfang , Wei Yubin , Wang Zhaowei , Zhang Tingting , Gong Weihua , Zhang Qinduan
Published: June 2022

A noise elimination method based on an improved particle swarm algorithm is applied to direct absorption spectroscopy. The algorithm combines the theory of spectral line shape to calculate a fitness function according to the original spectra. Comparing the particles and the fitness function to calculate the updating direction, and position of particles, the iterative update finally finds the optimal solution. The algorithm is applied to direct absorption spectroscopy to measure methane; compared with the signal without algorithm processing, the signal-to-noise ratio (SNR) is improved by 4.17 times, and the minimum detection limit in the experiment is 15.3 ppb. R2 = 0.9999 is calculated in the calibration experiment, and the error is less than 0.1 ppm in the repeatability experiment of constant methane at 2 ppm concentration.
Investigating the Effect of the Interaction of Maize Inducer and Donor Backgrounds on Haploid Induction Rates
Authors: Uliana Trentin Henrique , Batîru Grigorii , Frei Ursula Karoline , Dutta Somak , Lübberstedt Thomas
Published: June 2022

Doubled haploid technology is a feasible, fast, and cost-efficient way of producing completely homozygous lines in maize. Many factors contribute to the success of this system including the haploid induction rate (HIR) of inducer lines, the inducibility of donor background, and environmental conditions. Sixteen inducer lines were tested on eight different genetic backgrounds of five categories in different environments for the HIR to determine possible interaction specificity. The HIR was assessed using the R1-nj phenotype and corrected using the red root marker or using a gold-standard test that uses plant traits. RWS and Mo-17-derived inducers showed higher average induction rates and the commercial dent hybrid background showed higher inducibility. In contrast, sweet corn and flint backgrounds had a relatively lower inducibility, while non-stiff stalk and stiff stalk backgrounds showed intermediate inducibility. For the poor-performing donors (sweet corn and flint), there was no difference in the HIR among the inducers. Anthocyanin inhibitor genes in such donors were assumed to have increased the misclassification rate in the F1 fraction and, hence, result in a lower HIR.
Identification and Characterization of Colletotrichum fructicola and Colletotrichum siamense Causing Anthracnose on Luffa Sponge Gourd in China
Authors: Li Ping , Zhu Jun-Zi , Li Xiao-Gang , Zhong Jie
Published: June 2022

Luffa sponge gourd (Luffa cylindrica) is an important cucurbitaceous vegetable and is known as the source of loofah. From 2020 to 2021, a leaf disease occurred on luffa leaves in the Hunan Province of China. Symptoms were displayed as oval to irregular chlorotic lesions surrounded by yellow halos. The pathogens were isolated from the affected leaves. According to morphological characterization and molecular identification using multi-locus phylogenetic analysis of the internal transcribed spacer (ITS), actin (ACT), chitin synthase (CHS-1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-tubulin (TUB2), and partial mating type (Mat1-2) gene (ApMAT) regions, the pathogens were identified as two Colletotrichum species: Colletotrichum fructicola and C. siamense. Koch’s postulates were identified by a pathogenicity test and re-confirmation. To the best of our knowledge, C. fructicola and C. siamense are two new species associated with luffa sponge gourd anthracnose.
Deverra triradiata Hochst. ex Boiss. from the Northern Region of Saudi Arabia: Essential Oil Profiling, Plant Extracts and Biological Activities
Authors: Guetat Arbi , Abdelwahab Abdelrahman T. , Yahia Yassine , Rhimi Wafa , Alzahrani A. Khuzaim , Boulila Abdennacer , Cafarchia Claudia , Boussaid Mohamed
Published: June 2022

Devrra triradiata Hochst. ex Boiss is an occasional plant species in the Northern region of Saudi Arabia. The shrub is favored on sandy desert wadis, gypsaceous substrate, and sandy gravel desert. In folk medicine, the plant is used for many purposes; to relieve stomach pains, against intestinal parasites, and for the regulation of menstruation. The present study describes the chemical composition of the essential oils (EOs) of different plant parts of D. triradiata. In vivo and in vitro biological activities of plant extracts and essential oils were also studied. Phenylpropanoids, elemicin (flowers: 100%), dillapiole (Stems: 82.33%; and seeds: 82.61%), and apiol (roots: 72.16%) were identified as the major compounds. The highest antioxidant activity was recorded for the EOs of roots and stems (IC50 = 0.282 µg/mL and 0.706 µg/mL, respectively). For plant extracts, ethyl acetate showed the highest antioxidant activities (IC50 = 2.47 and 3.18 µg/mL). EOs showed high antifungal activity against yeasts with low azole susceptibilities (i.e., Malassezia spp. and Candida krusei). The MIC values of EOs ranged between 3.4 mg/mL and 56.4 mg/mL. The obtained results also showed phytotoxic potential of plant extracts both on the germination features of Triticum aestivum seeds and the vegetative growth of seedlings.
Potato Cultivar Identification in South Africa Using a Custom SNP Panel
Authors: Gazendam Inge , Mojapelo Pinkie , Bairu Michael W.
Published: June 2022

DNA fingerprinting is a molecular technique applied to identify genetic differences between plant cultivars or lines and is used for genetic purity testing. The suitability of single nucleotide polymorphism (SNP) panels for the fingerprinting of tetraploid potato were investigated as a new high throughput, objective, and cost-effective method instead of simple sequence repeats (SSRs) and polyacrylamide gel electrophoresis (PAGE). One-hundred and ninety (190) potato cultivars, including various cultivars currently important in South Africa, were genotyped at 500 SNP positions utilising SeqSNP by LGC Biosearch Technologies. An optimal panel of 25 SNP markers was identified that could discriminate between South African potato cultivars on genetic allele dosage. The genotypes of these SNPs were validated on selected potato genotypes using KASP (Kompetitive Allele Specific PCR) SNP assays. A database of SNP genotype profiles was compiled for all the entries of the germplasm database. The panel of 21 successful SNPs accurately identified the unique potato cultivars in the database. The KASP SNP assays of the successful SNP panel are therefore available for potato DNA fingerprinting as new germplasm, or purity test requests are submitted to ARC-VIMP. This panel provides an objective method for assigning putative cultivar identity to unknown samples submitted for fingerprinting.
The Involvement of Antioxidant Enzyme System, Nitrogen Metabolism and Osmoregulatory Substances in Alleviating Salt Stress in Inbred Maize Lines and Hormone Regulation Mechanisms
Authors: Wang Mingquan , Gong Shichen , Fu Lixin , Hu Guanghui , Li Guoliang , Hu Shaoxin , Yang Jianfei
Published: June 2022

Salt stress inhibited the growth of maize. B46 and NC236 were chosen as materials and NaCl concentrations (0, 55, 110, 165, and 220 mmol L−1) were set. We found the activities of SOD, POD, CAT, APX, GR, MDHAR, and DHAR decreased under NaCl stress. Compared with NC236, the contents of AsA and GSH, AsA/DHA and GSH/GSSG of B46 decreased. The content of O2−, H2O2, MDA, and EL of B46 increased. The contents of NO3− and NO2− decreased, while the content of NH4+ increased under high NaCl concentration. The activities of NR and NiR decreased, while the activities of GS and GOGAT increased first and then decreased. For B46 and NC236, the maximum of NADH-GDH and NAD-GDH appeared at 165 and 110 mmol L−1 NaCl concentration, respectively. Compared with B46, and the GOT and GPT activities of NC236 increased first and then decreased. With the increase of NaCl concentration, the contents of proline, soluble protein, and soluble sugar were increased. The Na+ content of B46 and NC236 increased, and the K+ content and K+/Na+ decreased. Compared with NC236, B46 had higher IAA content in leaf, higher Z + ZR content in leaf and root, and lower ABA content in leaf and root.
Ecophysiological Responses of Tall Wheatgrass Germplasm to Drought and Salinity
Authors: Borrajo Celina I. , Sánchez-Moreiras Adela M. , Reigosa Manuel J.
Published: June 2022

Tall wheatgrass (Thinopyrum ponticum (Podp.) Barkworth and D.R. Dewey) is an important, highly salt-tolerant C3 forage grass. The objective of this work was to learn about the ecophysiological responses of accessions from different environmental origins under drought and salinity conditions, to provide information for selecting superior germplasm under combined stress in tall wheatgrass. Four accessions (P3, P4, P5, P9) were irrigated using combinations of three salinity levels (0, 0.1, 0.3 M NaCl) and three drought levels (100%, 50%, 30% water capacity) over 90 days in a greenhouse. The control treatment showed the highest total biomass, but water-use efficiency (WUE), δ13C, proline, N concentration, leaf length, and tiller density were higher under moderate drought or/and salinity stress than under control conditions. In tall wheatgrass, K+ functions as an osmoregulator under drought, attenuated by salinity, and Na+ and Cl− function as osmoregulators under salinity and drought, while proline is an osmoprotector under both stresses. P3 and P9, from environments with mild/moderate stress, prioritized reproductive development, with high evapotranspiration and the lowest WUE and δ13C values. P4 and P5, from more stressful environments, prioritized vegetative development through tillering, showing the lowest evapotranspiration, the highest δ13C values, and different mechanisms for limiting transpiration. The δ13C value, leaf biomass, tiller density, and leaf length had high broad-sense heritability (H2), while the Na+/K+ ratio had medium H2. In conclusion, the combined use of the δ13C value, Na+/K+ ratio, and canopy structural variables can help identify accessions that are well-adapted to drought and salinity, also considering the desirable plant characteristics. Tall wheatgrass stress tolerance could be used to expand forage production under a changing climate.
Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic Brassica Allohexaploids
Authors: Ji Chengyan , Tian Zhaoran , Liu Yue , Shi Gongyao , Tian Baoming , Chen Weiwei , Xie Zhengqing , Han Xingzhou , Liang Niannian , Wei Fang , Wei Xiaochun
Published: June 2022

Polyploidy plays a crucial role in plant evolution and speciation. The development of male and female gametes is essential to the reproductive capacity of polyploids, but their gene expression pattern has not been fully explored in newly established polyploids. The present study aimed to reveal a detailed atlas of gene expression for gamete development in newly synthetic Brassica allohexaploids that are not naturally existing species. Comparative transcriptome profiling between developing anthers (staged from meiosis to mature pollen) and ovules (staged from meiosis to mature embryo sac) was performed using RNA-Seq analysis. A total of 8676, 9775 and 4553 upregulated differentially expressed genes (DEGs) were identified for the development of both gametes, for male-only, and for female-only gamete development, respectively, in the synthetic Brassica allohexaploids. By combining gene ontology (GO) biological process analysis and data from the published literature, we identified 37 candidate genes for DNA double-strand break formation, synapsis and the crossover of homologous recombination during male and female meiosis and 51 candidate genes for tapetum development, sporopollenin biosynthesis and pollen wall development in male gamete development. Furthermore, 23 candidate genes for mitotic progression, nuclear positioning and cell specification and development were enriched in female gamete development. This study lays a good foundation for revealing the molecular regulation of genes related to male and female gamete development in Brassica allohexaploids and provides more resourceful genetic information on the reproductive biology of Brassica polyploid breeding.
Mercury in Temperate Forest Soils and Suspended Sediments in the Connecticut River, Merrimack River, and Thames River Watersheds, USA
Authors: Richardson Justin , Mischenko Ivan , Butler Mark
Published: June 2022

Quantifying the sequestration in forest soils and watershed transport of Hg is key to reducing exposure of aquatic food chains to the toxic pollutant. We explored if forest soils, proximity in human developments, or watershed characteristics could shed light on sediment driven Hg transport in the Connecticut River, Merrimack River, and Thames River watersheds, in the northeastern USA. We sampled 48 upland forest soils and 32 riparian forest soils and collected bottled and trapped suspended sediments from April 2020 to November 2020 across the three watersheds. Forest soil Hg concentrations were greater in riparian forest soils (median = 153 ng g−1) than in upland forest soils (median = 71 ng g−1) across all three watersheds and developed sites near urbanization had higher Hg concentrations in the Merrimack River watershed (median = 407 ± 119 ng g−1). The Connecticut River had the highest suspended sediment Hg export rate but when normalized by total area of the watershed, the Merrimack River had 0.19 kg km−2 month−1 while the Connecticut River had 0.13 kg km−2 month−1 and 0.04 km−2 for the Thames River. Our findings suggest that riparian forest soils sequester while suspended sediments transport historical Hg pollution within the Merrimack River Watershed.
Characterization and Performance Enhancement of Cement-Based Thermoelectric Materials
Authors: Jani Ruchita , Holmes Niall , West Roger , Gaughan Kevin , Liu Xiaoli , Qu Ming , Orisakwe Esther , Stella Lorenzo , Kohanoff Jorge , Yin Hongxi , Wojciechowski Bartlomiej
Published: June 2022

Thermoelectric materials enable the direct conversion of thermal to electrical energy. One application of this is ambient heat energy harvesting where relatively stable temperature gradients existing between the inside and outside of a building could be utilized to produce electricity. Buildings can thus change from energy consumers to energy generators. This could ultimately help reduce the surface temperatures and energy consumption of buildings, especially in urban areas. In this paper, research work carried out on developing and characterizing a cement-based thermoelectric material is presented. Cement-based samples are doped with different metal oxides (Bi2O3 and Fe2O3) to enhance their thermoelectric properties, which are defined through their Seebeck coefficient, electrical conductivity and thermal conductivity. The study also discusses the positive impact of moisture content on the electrical conductivity
Adsorption of Cationic Pollutants from Water by Cotton Rope Coated with Cyclodextrin Polymers
Authors: Martwong Ekkachai , Sukhawipat Nathapong , Junthip Jatupol
Published: June 2022

The contamination from perilous organic compounds (pesticide and dyes) in water generates a significant problem for the environment and humans. A modified textile was prepared by a coating of anionic cyclodextrin polymer, obtained from the cross-linking between citric acid and β-cyclodextrin in the presence of poly (vinyl alcohol), on the cotton cord for cationic pollutant removal from an aqueous solution. Its physicochemical properties were also characterized by gravimetry, titration, stereomicroscopy, SEM, TGA, 13C NMR, and ATR-FTIR. The CC2 system exhibited 79.2% coating yield, 1.12 mmol/g COOH groups, 91.3% paraquat (PQ) removal, 97.0% methylene blue (MB) removal, and 98.3% crystal violet (CV) removal for 25 mg/L of initial concentration. The kinetics was fitted to the pseudo-second-order model using 6 h of contact time. The isotherm was suitable for the Langmuir isotherm with a maximum adsorption of 26.9 mg/g (PQ), 23.7 mg/g (MB), and 30.3 mg/g (CV). After 120 h of contact time in water and 5% v/v of HCI in ethanol, the weight loss was 7.5% and 5.6%, respectively. Finally, the recyclability performance reached 84.8% (PQ), 95.2% (MB), and 96.9% (CV) after five reuses.
Effects of Fucoidan Powder Combined with Mineral Trioxide Aggregate as a Direct Pulp-Capping Material
Authors: Kim Mijoo , Hayashi Marc , Yu Bo , Lee Thomas K. , Kim Reuben H. , Jo Deuk-Won
Published: June 2022

The development of direct pulp-capping materials with favorable biological and structural properties is an important goal in restorative dentistry. Fucoidan is a sulfated, fucose-containing polysaccharide obtained from brown seaweed, with a wide range of applications; however, its use as a direct pulp-capping material has not been examined. This study aimed to evaluate the mechanical, physical, and biological effects of fucoidan combined with conventional mineral trioxide aggregate (MTA) for direct pulp capping. The capping materials were created using Portland cement (80 wt%) and zirconium oxide (20 wt%) as base components, compared with base components plus 5 wt% fucoidan (PZF5) and base components plus 10 wt% fucoidan (PZF10). The initial and final setting time, compressive strength, chemical components, cell viability, adhesion, migration, osteogenesis, and gene expression were analyzed. Fucoidan significantly reduced the initial and final setting time, regardless of quantity. However, the compressive strength was lower for PZF5. Sulfur levels increased with fucoidan. The biological activity improved, especially in the PZF5 group. Cell migration, Alizarin Red S staining, and alkaline phosphatase activity were upregulated in the PZF5 group. Fucoidan is a useful regenerative additive for conventional pulp-capping materials because it reduces the setting time and improves cell migration and osteogenic ability.
Principles and Guidelines for In-Line Viscometry in Cereal Extrusion
Authors: Dalle Fratte Elia , D’hooge Dagmar R. , Eeckhout Mia , Cardon Ludwig
Published: June 2022

In the food industry, extrusion cooking finds numerous applications thanks to its high productivity and nutrient retention. More specifically, cereal extrusion, e.g., for savory snacks and breakfast products has an important market share. For such applications, rheology, which addresses viscous and elastic contributions, plays an important role in developing, optimizing, and controlling the extrusion manufacturing technique. In this context, conventional off-line rheometers are not ideal for providing data, as the goal is to replicate the exact thermomechanical history to which the food is subjected in the extrusion process. Hence, to achieve reliable analyses, in-line viscometers that have mostly been tested using oil-based polymers were introduced. Biopolymers (e.g., starch), however, are highly sensitive to both heat and mechanical degradation, and the viscometer design has to be adapted accordingly to produce an accurate measurement. Alongside a discussion of the different designs available, this review will address the most common methodologies for measuring the steady shear viscosity, extensional viscosity, and the first normal stress difference for food applications, providing researchers in the biopolymer and food engineering fields with a general introduction to this emerging topic.
Polymers Based on PLA from Synthesis Using D,L-Lactic Acid (or Racemic Lactide) and Some Biomedical Applications: A Short Review
Authors: de França Juliene Oliveira Campos , da Silva Valadares Deborah , Paiva Mateus Freitas , Dias Sílvia Cláudia Loureiro , Dias José Alves
Published: June 2022

Poly(lactic acid) (PLA) is an important polymer that is based on renewable biomass resources. Because of environmental issues, more renewable sources for polymers synthesis have been sought for industrial purposes. In this sense, cheaper monomers should be used to facilitate better utilization of less valuable chemicals and therefore granting more sustainable processes. Some points are raised about the need to study the total degradability of any PLA, which may require specific composting conditions (e.g., temperature, type of microorganism, adequate humidity and aerobic environment). Polymerization processes to produce PLA are presented with an emphasis on D,L-lactic acid (or rac-lactide) as the reactant monomer. The syntheses involving homogeneous and heterogeneous catalytic processes to produce poly(D,L-Lactic acid) (PDLLA) are also addressed. Additionally, the production of blends, copolymers, and composites with PDLLA are also presented exemplifying different preparation methods. Some general applications of these materials mostly dedicated to the biomedical area over the last 10–15 years will be pointed out.
Research on Tensile Properties of Carbon Fiber Composite Laminates
Authors: Wang Jiayi , Chen Lifeng , Shen Wei , Zhu Lvtao
Published: June 2022

In order to study the thread tensile performance of carbon fiber composite laminates, the connection between the test piece, connecting bolts, bushings, and the composite matrix, was leveraged for loading, and combined with an ultra-sound scanning imaging system, experiments were carried out on the dynamic response to record the failure behavior of the laminate structure of equal thickness. The effects of different pull-off loading strengths on the dynamic failure process, deformation profile, midpoint deformation, failure mode, and energy dissipation ratio of the thread were studied. The results show that (1) with the increase in pull-off strength, the response speed of mid-point deformation increases, the thread deformation mode changes from overall deformation to partial deformation, and the localized effect increases, accompanied by severe matrix and fiber fracture failure; (2) the thread energy dissipation ratio ascends with increasing pull-off strength and exhibits three distinct stages, i.e., elastic deformation, central fracture, and complete failure, which are directly related to the structural failure mode; (3) the failure load increases with the increment of the thickness of the laminate, and the maximum failure surface of the specimen will move from the upper layer of the laminate to the lower layer along the thickness direction; (4) the deformation velocity of the midpoint augments with the increase in the tensile rate, which can be included as a factor to assess the tensile properties of carbon fiber composites.
Characterizing the Bounce and Separation Dynamics of Janus Drop on Macrotextured Surface
Authors: Choi WooSeok , Yun Sungchan
Published: June 2022

Janus drops are thermodynamically stable when a high-viscosity fluid is imposed on a low-viscosity fluid. To understand physical mechanisms in Janus drop impact on macrotextured surfaces, several challenges in finding parameters or strategies still remain. Here, this study investigates the asymmetric bounce and separation of impinging Janus drops on non-wettable surfaces decorated with a macroridge to explore the effect of the drop size, viscosity ratio, and ridge size on the dynamics. Through numerical simulations, we determine the threshold Weber number, above which separation occurs, by varying drop diameters and viscosity ratios of the Janus drops. We investigate the initial bouncing directions of separated drops as a function of the impact velocity and viscosity ratio. We also predict how the separation efficiency is affected by the ridge’s height and width. The asymmetric impact dynamics of Janus drops on macrotextured surfaces can provide new strategies to control drop bouncing in applications, such as liquid separation and purification.
PEEK in Fixed Dental Prostheses: Application and Adhesion Improvement
Authors: Wang Biyao , Huang Minghao , Dang Pengrui , Xie Jiahui , Zhang Xinwen , Yan Xu
Published: June 2022

Polyetheretherketone (PEEK) has been widely applied in fixed dental prostheses, comprising crowns, fixed partial dentures, and post-and-core. PEEK’s excellent mechanical properties facilitate better stress distribution than conventional materials, protecting the abutment teeth. However, the stiffness of PEEK is not sufficient, which can be improved via fiber reinforcement. PEEK is biocompatible. It is nonmutagenic, noncytotoxic, and nonallergenic. However, the chemical stability of PEEK is a double-edged sword. On the one hand, PEEK is nondegradable and intraoral corrosion is minimized. On the other hand, the inert surface makes adhesive bonding difficult. Numerous strategies for improving the adhesive properties of PEEK have been explored, including acid etching, plasma treatment, airborne particle abrasion, laser treatment, and adhesive systems.
Synthesis and Characterization of Green ZnO@polynaniline/Bentonite Tripartite Structure (G.Zn@PN/BE) as Adsorbent for As (V) Ions: Integration, Steric, and Energetic Properties
Authors: Abdel Salam Mohamed , Mokhtar Mohamed , Albukhari Soha M. , Baamer Doaa F. , Palmisano Leonardo , Jaremko Mariusz , Abukhadra Mostafa R.
Published: June 2022

A green ZnO@polynaniline/bentonite composite (G.Zn@PN/BE) was synthesized as an enhanced adsorbent for As (V) ions. Its adsorption properties were assessed in comparison with the integrated components of bentonite (BE) and polyaniline/bentonite (PN/BE) composites. The G.Zn@PN/BE composite achieved an As (V) retention capacity (213 mg/g) higher than BE (72.7 mg/g) and PN/BE (119.8 mg/g). The enhanced capacity of G.Zn@PN/BE was studied using classic (Langmuir) and advanced equilibrium (monolayer model of one energy) models. Considering the steric properties, the structure of G.Zn@PN/BE demonstrated a higher density of active sites (Nm = 109.8 (20 °C), 108.9 (30 °C), and 67.8 mg/g (40 °C)) than BE and PN/BE. This declared the effect of the integration process in inducing the retention capacity by increasing the quantities of the active sites. The number of adsorbed As (V) ions per site (1.76 up to 2.13) signifies the retention of two or three ions per site by a multi-ionic mechanism. The adsorption energies (from −3.07 to −3.26 kJ/mol) suggested physical retention mechanisms (hydrogen bonding and dipole bonding forces). The adsorption energy, internal energy, and free enthalpy reflected the exothermic, feasible, and spontaneous nature of the retention process. The structure is of significant As (V) uptake capacity in the existence of competitive anions or metal ions.
Chitosan: A Sustainable Material for Multifarious Applications
Authors: Hameed Abdul Zubar , Raj Sakthivel Aravind , Kandasamy Jayakrishna , Baghdadi Majed Abubakr , Shahzad Muhammad Atif
Published: June 2022

Due to the versatility of its features and capabilities, chitosan generated from marine crustacean waste is gaining importance and appeal in a wide variety of applications. It was initially used in pharmaceutical and medical applications due to its antibacterial, biocompatible, and biodegradable properties. However, as the demand for innovative materials with environmentally benign properties has increased, the application range of chitosan has expanded, and it is now used in a variety of everyday applications. The most exciting aspect of the chitosan is its bactericidal properties against pathogens, which are prevalent in contaminated water and cause a variety of human ailments. Apart from antimicrobial and water filtration applications, chitosan is used in dentistry, in water filtration membranes to remove metal ions and some heavy metals from industrial effluents, in microbial fuel cell membranes, and in agriculture to maintain moisture in fruits and leaves. It is also used in skin care products and cosmetics as a moisturizer, in conjunction with fertilizer to boost plant immunity, and as a bi-adhesive for bonding woods and metals. As it has the capacity to increase the life span of food items and raw meat, it is an unavoidable component in food packing and preservation. The numerous applications of chitosan are reviewed in this brief study, as well as the approaches used to incorporate chitosan alongside traditional materials and its effect on the outputs.
Recent Advancements in Cyclodextrin-Based Adsorbents for the Removal of Hazardous Pollutants from Waters
Authors: Syeda Shan E. Zehra , Nowacka Dominika , Khan Muhammad Shahzeb , Skwierawska Anna Maria
Published: June 2022

Water is an essential substance for the survival on Earth of all living organisms. However, population growth has disturbed the natural phenomenon of living, due to industrial growth to meet ever expanding demands, and, hence, an exponential increase in environmental pollution has been reported in the last few decades. Moreover, water pollution has drawn major attention for its adverse effects on human health and the ecosystem. Various techniques have been used to treat wastewater, including biofiltration, activated sludge, membrane filtration, active oxidation process and adsorption. Among the mentioned, the last method is becoming very popular. Moreover, among the sorbents, those based on cyclodextrin have gained worldwide attention due to their excellent properties. This review article overviewed recent contributions related to the synthesis of Cyclodextrin (CD)-based adsorbents to treat wastewater, and their applications, especially for the removal of heavy metals, dyes, and organic pollutants (pharmaceuticals and endocrine disruptor chemicals). Furthermore, new adsorption trends and trials related to CD-based materials are also discussed regarding their regenerative potential. Finally, this review could be an inspiration for new research and could also anticipate future directions and challenges associated with CD-based adsorbents.
Synthesis and Characterization of Thiol-Functionalized Polynorbornene Dicarboximides for Heavy Metal Adsorption from Aqueous Solution
Authors: Onchi Alejandro , Corona-García Carlos , Santiago Arlette A. , Abatal Mohamed , Soto Tania E. , Alfonso Ismeli , Vargas Joel
Published: June 2022

The contamination of water resources with heavy metals is a very serious concern that demands prompt and effective attention due to the serious health risks caused by these contaminants. The synthesis and ring-opening metathesis polymerization (ROMP) of norbornene dicarboximides bearing thiol pendant groups, specifically, N-4-thiophenyl-exo-norbornene-5,6-dicarboximide (1a), N-4-(methylthio)phenyl-exo-norbornene-5,6-dicarboximide (1b) and N-4-(trifluoromethylthio)phenyl-exo-norbornene-5,6-dicarboximide (1c), as well as their assessment for the removal of heavy metals from aqueous systems, is addressed in this work. The polymers were characterized by NMR, SEM and TGA, among others. Single and multicomponent aqueous solutions of Pb2+, Cd2+ and Ni2+ were employed to perform both kinetic and isothermal adsorption studies taking into account several experimental parameters, for instance, the initial metal concentration, the contact time and the mass of the polymer. In general, the adsorption kinetic data fit the pseudo-second-order model more efficiently, while the adsorption isotherms fit the Freundlich and Langmuir models. The maximum metal uptakes were 53.7 mg/g for Pb2+, 43.8 mg/g for Cd2+ and 29.1 mg/g for Ni2+ in the SH-bearing polymer 2a, 46.4 mg/g for Pb2+, 32.9 mg/g for Cd2+ and 27.1 mg/g for Ni2+ in the SCH3-bearing polymer 2b and 40.3 mg/g for Pb2+, 35.9 mg/g for Cd2+ and 27.8 mg/g for Ni2+ in the SCF3-bearing polymer 2c, correspondingly. The better performance of polymer 2a for the metal uptake was ascribed to the lower steric hindrance and higher hydrophilicity imparted by –SH groups to the polymer. The results show that these thiol-functionalized polymers are effective adsorbents of heavy metal ions from aqueous media.
Modulation of Molecular Structure and Mechanical Properties of κ-Carrageenan-Gelatin Hydrogel with Multi-Walled Carbon Nanotubes
Authors: Gubaidullin Aidar , Makarova Anastasiya , Derkach Svetlana , Voron’ko Nicolai , Kadyirov Aidar , Ziganshina Sufia , Salnikov Vadim , Zueva Olga , Zuev Yuri
Published: June 2022

Hydrogels, three-dimensional hydrophilic water-insoluble polymer networks having mechanical properties inherent for solids, have attracted continuous research attention over a long time period. Here, we studied the structure and properties of hydrogel based on gelatin, κ-carrageenan and CNTs using the combination of SAXS, PXRD, AFM microscopy, SEM and rheology methods. We have shown that the integration of polysaccharide and protein in the composite hydrogel leads to suppression of their individual structural features and homogenization of two macromolecular components into a single structural formation. According to obtained SAXS results, we observed the supramolecular complex, which includes both polysaccharide and protein components associated with each other. It was determined that hydrogel structure formed in the initial solution state (dispersion) retains hydrogel supramolecular structure under its cooling up to gel state. The sizes of dense cores of these polyelectrolyte complexes (PEC) slightly decrease in the gel state in comparison with PEC water dispersion. The introduction of CNTs to hydrogel does not principally change the type of supramolecular structure and common structural tendencies observed for dispersion and gel states of the system. It was shown that carbon nanotubes embedded in hydrogel act as the supplementary template for formation of the three-dimensional net, giving additional mechanical strengthening to the studied system.
In Vitro Tissue Reconstruction Using Decellularized Pericardium Cultured with Cells for Ligament Regeneration
Authors: Suzuki Mika , Kimura Tsuyoshi , Yoshida Yukina , Kobayashi Mako , Hashimoto Yoshihide , Takahashi Hironobu , Shimizu Tatsuya , Anzai Shota , Nakamura Naoko , Kishida Akio
Published: June 2022

Recent applications of decellularized tissues have included the ectopic use of their sheets and powders for three-dimensional (3D) tissue reconstruction. Decellularized tissues are fabricated with the desired functions to employ them to a target tissue. The aim of this study was to develop a 3D reconstruction method using a recellularized pericardium to overcome the difficulties in cell infiltration into tight and dense tissues, such as ligament and tendon tissues. Decellularized pericardial tissues were prepared using the high hydrostatic pressurization (HHP) and surfactant methods. The pericardium consisted of bundles of aligned fibers. The bundles were slightly disordered in the surfactant decellularization method compared to the HHP decellularization method. The mechanical properties of the pericardium were maintained after the HHP and surfactant decellularizations. The HHP-decellularized pericardium was rolled up into a cylindrical formation. Its mechanical behavior was similar to that of a porcine anterior cruciate ligament in tensile testing. NIH3T3, C2C12, and mesenchymal stem cells were adhered with elongation and alignment on the HHP- and surfactant-decellularized pericardia, with dependences on the cell type and decellularization method. When the recellularized pericardium was rolled up into a cylinder formation and cultured by hanging circulation for 2 days, the cylinder formation and cellular elongation and alignment were maintained on the decellularized pericardium, resulting in a layer structure of cells in a cross-section. According to these results, the 3D-reconstructed decellularized pericardium with cells has the potential to be an attractive alternative to living tissues, such as ligament and tendon tissues.
Development of Aloe Vera-Green Banana Saba-Curcumin Composite Film for Colorimetric Detection of Ferrum (II)
Authors: Vonnie Joseph Merillyn , Jing Ting Bong , Rovina Kobun , Erna Kana Husna , Felicia Wen Xia Ling , Nur ‘Aqilah Nasir Md , Abdul Wahab Roswanira
Published: June 2022

This study was performed to develop and characterize a bio-film composed of Aloe vera (Aloe barbadensis), green banana Saba (Musa acuminata x balbisiana), and curcumin for the detection of Fe2+ ions. Cross-linking interaction between banana starch-aloe vera gel and banana starch-curcumin enhanced l the sensing performance of the composite film towards divalent metal ions of Fe2+. The morphological structure of the Aloe vera-banana starch-curcumin composite revealed a smooth and compact surface without cracks and some heterogeneity when observed under Scanning Electron Microscopy (SEM). The thickness, density, color property, opacity, biodegradation, moisture content, water-solubility, water absorption, swelling degree, and water vapor permeability of bio-films were measured. The incorporation of aloe vera gel and curcumin particles onto the banana starch film has successfully improved the film properties. The formation of the curcumin-ferrum (II) complex has triggered the film to transform color from yellow to greenish-brown after interaction with Fe2+ ions that exhibit an accuracy of 101.11% within a swift reaction time. Good linearity (R2 = 0.9845) of response on colorimetric analysis was also obtained in Fe2+ ions concentration that ranges from 0 to 100 ppm, with a limit of detection and quantification found at 27.84 ppm and 92.81 ppm, respectively. In this context, the film was highly selective towards Fe2+ ions because no changes of color occur through naked eye observation when films interact with other metal ions, including Fe3+, Pb2+, Ni2+, Cd2+, and Cu2+. Thus, these findings encourage curcumin-based starch films as sensing materials to detect Fe2+ ions in the field of food and agriculture.
Influence of Atmospheric Conditions on Mechanical Properties of Polyamide with Different Content of Recycled Material in Selective Laser Sintering
Authors: Pilipović Ana , Ilinčić Petar , Bakić Ante , Kodvanj Janoš
Published: June 2022

The price of material is an important factor when selecting the additive polymer procedure. In selective laser sintering (SLS), the price can be reduced by the recycling of material, i.e., with different shares of original and recycled material, as well as by the orientation of the product during manufacturing. Numerous tests warn that orientation in the direction of z axis should be as low as possible to reduce the total price of the product. The product also has to satisfy the influence of atmospheric conditions to which it is exposed during its lifetime, i.e., UV radiation and humid environment. UV light, with sun being its most common source, and average humidity in different parts of the world can be approximately from 20% to 90%, depending on time, day and geographic location. In this work, the test specimens have been made of original, mixed and 100% recycled material and then exposed to the influences of UV radiation and water absorption. After having been exposed to atmospheric conditions for a longer time, the mechanical properties of the polyamide products made by selective laser sintering were tested. The results show that exposure to UV radiation reduces tensile elongation at all ratios of recycled material and orientation of 70–90% except in the z direction, while in flexural deformation it is the other way around. The effect of water was observed only between the 7th–14th day of absorption with a decrease in strength until the deformation did not change.