bluuebunny/crossref_metadata_2025 huggingface dataset:
Women care in situations of sexual violence: an integrative literature review
Authors: Trentin Daiane , Vargas Mara Ambrosina de Oliveira , Lino Monica Motta , Leal Sandra Maria Cezar , Ferreira Micheli Leal , Saioron Isabela
Published: October 2019

Abstract Objective: to identify Brazilian and international scientific evidence about women in situation of sexual violence service by a multidisciplinary health team. Method: an integrative literature review carried out in four databases and in a digital library, with the following inclusion criteria: type of study, language and temporal cut. The final sample consisted of 34 studies. Results: nine categories emerged: service network; teamwork; health professional in the service network; qualification and training; comprehensiveness; protocols; services; access to services and support from managers. They were organized into potentialities, demands and weaknesses, constituting elements necessary for assistance effectiveness by the multidisciplinary team to women in situation of sexual violence. Conclusion an Implications for practice: despite the potential of providing women care in situations of sexual violence, weaknesses stand out as an incipient and inexistent articulation of the intersectoral care network, generating demands for the networks’ construction, articulation and sustainability. In addition, professional qualification and support of managers in the performance of public policies make possible care comprehensiveness, updates and service evidence.
ICMS Ecológico e desmatamento nos municípios prioritários do estado do Pará
Authors: Tupiassu Lise , Fadel Luiz Paulo de Sousa Leão , Gros-Désormeaux Jean-Raphaël
Published: October 2019

Resumo O ICMS Ecológico consiste no dimensionamento, segundo critérios ambientais, de parte dos repasses da parcela do Imposto sobre Circulação de Mercadorias e Serviços que cabe aos municípios. A política se presta à compensação e incentivo de ações favoráveis ao meio ambiente e foi introduzida no Pará com o objetivo explícito de contribuir para a redução das altas taxas de desmatamento, a partir do fomento à atuação municipal nesse propósito. Este estudo pretende verificar se os efeitos redistributivos iniciais operados pela política ocorreram em consonância com a finalidade almejada. Realizou-se análise da destinação dos recursos recebidos em razão da política, dos gastos municipais com gestão ambiental e da ingerência territorial municipal para compreender o impacto da introdução do critério ecológico de repartição sobre os municípios com altas taxas de desmatamento incluídos na Lista de Municípios Prioritários do Ministério do Meio Ambiente. Os resultados indicam que os municípios prioritários paraenses foram beneficiados com a introdução do ICMS Ecológico no estado sem que a política tenha implicado maior engajamento municipal no combate ao desmatamento. Conclui-se que houve certa incoerência entre os efeitos redistributivos operados pelos ICMS Ecológico paraense e o objetivo de redução do desmatamento, bem como com a base teórica da política.
PHYSICAL ACTIVITY IN DAILY LIFE, EXERCISE CAPACITY AND QUALITY OF LIFE IN PATIENTS WITH CROHN’S DISEASE ON INFLIXIMAB-INDUCED REMISSION: A PRELIMINARY STUDY
Authors: CABALZAR Andrea Lemos , AZEVEDO Felipe Meirelles de , LUCCA Fernando de Azevedo , REBOREDO Maycon de Moura , MALAGUTI Carla , CHEBLI Júlio Maria Fonseca
Published: October 2019

ABSTRACT BACKGROUND: Physical activity in daily life and exercise capacity have not been assessed in patients with Crohn’s disease to date. OBJECTIVE: To evaluate the physical activity in daily life, exercise capacity, quality of life, and prevalence of mood disorders in patients with moderate-to-severe Crohn’s disease on infliximab-induced remission and the possible associations among variables. METHODS: A cross-sectional preliminary study was conducted. Twenty-six patients with Crohn’s disease and 20 controls were selected. Participants underwent evaluation of physical activity in daily life (triaxial accelerometer), exercise capacity (shuttle walk test), handgrip strength, quality of life, and presence of mood disorders. RESULTS: The number of steps taken (7446±3081 vs 7898±2487), active time (80.6±42 vs 89.7±24.3min), shuttle walk test distance [665 (405) vs 710 (409) m] and handgrip strength [31 (15) vs 29 (20) kgf did not show any difference between the patients with Crohn’s disease and the controls. The time spent lying down [95.8 (68.8) vs 60.9 (74.7) min] was greater and some domains of the quality of life were superior in the patients with Crohn’s disease. No correlation was observed between the physical activity in daily life and quality of life or presence of mood disorders in patients with Crohn’s disease. CONCLUSION: Patients with Crohn’s disease on infliximab-induced remission, despite to more time spent lying down, they have the same level of physical activity in daily life and exercise capacity min compared with the controls.
NUTRITIONAL INDICATORS OF MALNUTRITION IN HOSPITALIZED PATIENTS
Authors: LEANDRO-MERHI Vânia Aparecida , COSTA Caroline Lobo , SARAGIOTTO Laiz , AQUINO José Luiz Braga de
Published: October 2019

ABSTRACT BACKGROUND: Malnutrition is associated with clinical factors, including longer hospital stay, increased morbidity and mortality and hospital costs. OBJECTIVE: To investigate the prevalence of malnutrition using different nutritional indicators and to identify factors that contribute to malnutrition in hospitalized patients. METHODS: We investigated anthropometric, laboratory standards, nutritional risk screening (NRS), subjective global assessment (SGA), mini nutritional assessment and habitual energy consumption (HEC). Chi-square, Fisher’s exact test, Mann-Whitney test and univariate and multiple Cox regression analysis were used, at 5% significance level. RESULTS: It was found 21.01% of malnourished individuals by ASG; a total of 34.78% with nutritional risk according to NRS and 11.59% with low weight (BMI). There was no statistically significant difference in the prevalence of malnutrition by ASG (P=0.3344) and nutritional risk by NRS (P=0.2286), among the types of disorders. Patients with nutritional risk were of higher median age (64.5 vs 58.0 years; P=0.0246) and had lower median values of HEC (1362.1 kcal vs 1525 kcal, P=0.0030), of calf circumference (32.0 cm vs 33.5 cm, P=0.0405) of lymphocyte count (1176.5 cell/mm3 vs 1760.5 cell/ mm3, P=0.0095); and higher percentage of low body weight according to the BMI (22.9% vs 5.6%; P=0.0096). Lymphocyte count was associated with nutritional risk (P=0.0414; HR= 1.000; IC95%= 0.999; 1.000). CONCLUSION: NRS was more sensitive than other indicators in the diagnosis of malnutrition. Patients at risk were older and had lower HEC values, calf circumference, BMI and lymphocyte count. Low lymphocyte count was considered a factor associated with nutritional risk by the NRS.
A produção científica dos coordenadores do Grupo de Trabalho Currículo da Associação Nacional de Pós-Graduação e Pesquisa em Educação (ANPEd): opções teórico-metodológicas
Authors: Matos Cleide Carvalho
Published: October 2019

RESUMO Este artigo analisa as perspectivas teórico-metodológicas da produção científica dos coordenadores do Grupo de Trabalho (GT) 12 (Currículo) da Associação Nacional de Pós-Graduação e Pesquisa em Educação (ANPEd) (2000 a 2010) e os impactos dessa produção nos artigos científicos publicados no referido GT. Encontramos em Bourdieu (1983, 2004) a ancoragem para a consecução da análise. A maior parte da produção científica examinada tem como quadro de referências a perspectiva pós-crítica. No interior desse campo científico - assimetricamente organizado e estruturado por grupos detentores de maior ou menor capital social e, portanto, em constante disputa pela hegemonia científica -, tal perspectiva alcançou, no período analisado, considerável aceitação. A análise permitiu concluir que os coordenadores do GT são referências para o campo, influenciando o desenvolvimento de novas pesquisas, que oxigenam a produção do conhecimento.
ANPEd: rumo a meio século de lutas por educação e democracia
Authors: Souza Maria Antônia de
Published: October 2019

RESUMO O objetivo deste artigo é problematizar o papel da Associação Nacional de Pós-Graduação e Pesquisa em Educação (ANPEd) no conjunto das lutas por educação e democracia. A associação tem sido resistência a políticas conservadoras e privatistas na educação e mediadora na construção de políticas públicas efetivamente democráticas. O trabalho resulta de análise documental de boletins, de conteúdos dos grupos de trabalho (GTs) e de notícias divulgadas na página da associação na web. A luta por educação e emancipação humana está presente na prática política da ANPEd, que, por sua vez, se estabelece como voz coletiva de resistência a pautas hegemônicas. O movimento de constituição dos GTs evidencia o posicionamento político da associação, ao mesmo tempo em que revela a constituição de objetos de investigação por meio das práticas sociais.
Boletins da ANPEd: das possibilidades de problematização das questões educacionais para além dos espaços acadêmicos
Authors: Ferreira Helena Maria , Lima Francine de Paulo Martins , Villarta-Neder Marco Antonio
Published: October 2019

RESUMO O presente artigo busca caracterizar o papel de boletins publicados pela Associação Nacional de Pós-Graduação e Pesquisa em Educação (ANPEd) para a qualificação do debate em educação. Para tal, elegemos como objeto de análise 10 boletins publicados pela ANPEd nos anos 2018 e 2019. Foi possível constatar que a associação demonstra, na organização dos boletins, um enfrentamento ético e político às questões que dizem respeito ao contexto da educação brasileira. Nesse posicionamento, enquanto ato pelo qual se responsabiliza perante a educação brasileira, os boletins configuram-se em uma abordagem que, por meio de enunciados que dialogam com saberes, práticas e dizeres, ultrapassa a dimensão de divulgar e/ou informar. Isso representa uma concepção do fazer acadêmico como um compromisso indissociável entre a produção de saberes especializados, o diálogo desses saberes com outros saberes produzidos e circulantes no interior da sociedade e as fronteiras, no âmbito educacional, entre o conhecer e o agir.
Why has sustainability advanced in management schools? A sociological explanation
Authors: Lessa Bruno de Souza , Souza Ana Clara Aparecida Alves de , Lopes Fernando Dias
Published: October 2019

Abstract This theoretical essay aims to provide a sociological explanation for the increasing importance of sustainability principles and practices in Management Schools. It does so by approximating two theoretical perspectives. First, from a macrosociological standpoint, it employs some of Pierre Bourdieu’s core concepts and his theory of the State to interpret how sustainability-related principles and practices have advanced in Management Schools, theorizing about the context of developed countries. Second, it changes the focus onto a microsociological perspective to analyze how these advances have been taking place in Brazil. In order to do so, it utilizes Bernard Lahire’s conceptual apparatus and his sociology at an individual scale. Therefore, this essay supports a double-folded argument. On the one hand, it claims that in developed countries the State has been the protagonist for the advancement of sustainability in Management Schools, since the State has the power over monopolies that are fundamental to engender social changes at national level. On the other hand, when its focus is shifted onto Brazilian reality, it argues that the State has a smaller participation regarding the advancements of sustainability-related practices and principles in Management Schools. Thus, the essay claims it is necessary to change the focus to the importance of key individuals, who have been essential for such advances.
Chromosome 3DL Arm Assembly and Annotation v2
Authors: Lu Fu-Hao , McKenzie Neil , Gardiner Laura-Jayne , Luo Ming-Cheng , Hall Anthony , W Bevan Michael
Published: October 2019

This protocol descriobed the detailed procedures how we made the chromosome 3D long arm pseudomolecule in hexaploid wheat (Triticum aestivum, AABBDD).
Transcription Analyses v1
Authors: Lu Fu-Hao , McKenzie Neil , Gardiner Laura-Jayne , Luo Ming-Cheng , Hall Anthony , W Bevan Michael
Published: October 2019

This protocol describes how we preccessed the RNAseq data of wheat and Aegilops tauschii.
ORTAOKUL ÖĞRENCİLERİNİN SALDIRGANLIK DURUMLARI VE OYNADIKLARI BİLGİSAYAR OYUNLARI
Authors: Evgin Derya , (ışık) Çalışkan Zehra , Kaplan Bahriye , Caner Nuray
Published: October 2019

GİRİŞ: Bu araştırma, ortaokul öğrencilerinin şiddet davranışları ve
oynadıkları bilgisayar oyunlarını belirlemek amacıyla yapılmıştır. YÖNTEM: Kesitsel tanımlayıcı tipte olan çalışma
239 ortaokul öğrencisi ile yapılmıştır. Çalışma için kurum, ebeveyn ve öğrenci
onamı alınmış, veriler anket formu ve Saldırganlık Ölçeği (SÖ) ile yüz-yüze toplanmıştır. Verilerin
değerlendirilmesinde tanımlayıcı
istatistikler ve ki-kare testi kullanılmıştır. BULGULAR: Araştırmaya
katılan öğrencilerin Öğrencilerin % 43.9’u son bir yıl içinde şiddete maruz
kaldığını, %42.3’ü de şiddet uyguladığını ifade etmişlerdir. Öğrenciler en çok
itilme, tekmelenme, tokatlanma (%79.5), bir alet ile saldırma (%79.1), tehdit
edilmeyi (%75.7), en az ise lakap takılmasını (%40.2) şiddet olarak
değerlendirmişlerdir. Ayrıca öğrencilerin % 88.7’ sinin evinde bilgisayar
olduğu, %85.8’ inin bilgisayar oyunu oynadığı ve %10.7’ sinin 2 saatten daha
uzun süre bilgisayarda oyun oynadığı belirlenmiştir. Kız öğrencilerin %39.8’ i
bilgisayarda zeka ve mantık oyunları oynarken, erkek öğrencilerin %61.7’ sinin
savaş ve strateji oyunları oynadıkları bulunmuştur. Öğrencilerin saldırganlık
ölçeğinden aldıkları toplam puanların ortalamasının 80.1±2.53 olduğu bulunmuştur.
Sınıf düzeyi arttıkça saldırganlık düzeyinde artış görülmektedir (p<
0.05).  Saldırganlık düzeyi yüksek olan
öğrencilerin daha çok (% 73.3) savaş ve strateji oyunları oynadıkları tespit
edilmiştir ( p<0.05). 

SONUÇ: Bu sonuçlar
doğrultusunda; ailelere; şiddet, şiddet davranışları ve bilgisayar oyunlarının
çocuğa yönelik etkileriyle ilgili disiplinler arası işbirliği yapılarak
eğitimler verilmesi önerilmektedir.
Prevalencia y determinantes de tuberculosis pulmonar en un centro carcelario de Cali Colombia
Authors: Muegues Sirley Isabel , Núñez Constanza , Luna Lucy , Pacheco Robinson
Published: October 2019

Antecedentes: La Tuberculosis Pulmonar (TBp) es un problema de salud pública en el mundo y se exacerba en los centros carcelarios.

Objetivo: Determinar prevalencia y factores de riesgo de la trasmisión de TBp en un centro carcelario de Cali, Colombia, 2013 - 2014.

Métodos: Estudio de casos y controles. El registro institucional de la cárcel de Villahermosa, reportó 5.815 personas privadas de la libertad en el periodo del estudio; de estos 98 fueron casos y 98 controles.

Resultados: La prevalencia de TBp fue de 1.7%. La mediana de edad 29.8 años (RIQ 23.0-33.5), la etnia predominante fue la mestiza con 55% (108/196), estado civil soltero 57% (112/196), nivel educativo secundaria 75% (129/196), nivel socioeconómico dos, 71% (138/196), aseguramiento subsidiado 99% (194/196) y ocupación en construcción 71% (123/196). La probabilidad de desarrollar TBp en la cárcel de Villahermosa es explicada por desnutrición (OR = 17.5 IC 95% 4.06-76.8,), y la infección con VIH (OR= 8.9 IC 95% 1.07-74.2).

Conclusiones: La dinámica de la trasmisión de la TBp está determinada no solo por las condiciones de salud de los individuos, sino, además, por las características del entorno donde estos se interactúan como seres sociales. Los programas de control de TB deben realizar análisis de indicadores en los centros carcelarios de manera independiente al de la población general a fin de implementar estrategias de prevención y control enfocadas en la gestión del riesgo.
Overview of this issue
Authors: Barham Lyn , Stewart Michelle
Published: October 2019

Not available
Plan: Me – a practical tool for career decision making
Authors: Dallison Katie J
Published: October 2019

Due to the realities of modern career service provision within most universities, clients attend short (often one-off) career interventions. Hence, practitioners require simple, adaptable tools that are underpinned by career theory and can be explained easily, and empower clients to progress through their career journey independently. This paper explains a tool that has been developed from theory and through practice, and is now positioned to become the subject of further research and formal evaluation. This tool, referred to in the article as ‘Plan: Me’, takes a holistic approach to career decision making, integrating goal setting and allowing clients to map out a process of how they move themselves forward.
Aromatic complexity in Verdicchio wines: a case study
Authors: Carlin Silvia , Vrhovsek Urska , Lonardi Andrea , Landi Lorenzo , Mattivi Fulvio
Published: October 2019

Aim: Verdicchio is a white wine grape variety that has been cultivated for hundreds of years in the Marche region of central Italy. Verdicchio is used to produce all kinds of dry, sweet and sparkling wines, some of which can be aged for ten or more years. This study aimed to extend knowledge of the volatile profile of Verdicchio wines and the recognition and detection of odorous molecules. We considered wines produced in multiple vintages from some of the best Cru from the Marche region, in the Castelli di Jesi Classico area.Methods and results: Two data sets were considered: a vertical collection that included wines from different vintages, same variety, different production areas vinified by the same winery and a horizontal collection of wines from the 2016 harvest, considering different production areas, harvest times and clones produced by the same winery. Samples were analysed with GC×GC-ToF-MS, GC-MS-MS and GC-O. Comprehensive profiling with more than 1000 compounds allowed the wines produced in different areas to be separated. By GC-O analysis 48 main odorants were found. This survey led to the identification of 3-methyl-2,4-nonanedione (3-MND) that impart an anise note and an interesting content in methyl salicylate as a possible key odorant characteristic in Verdicchio.Conclusion: The volatile profile of different Verdicchio wines from the best production areas was investigated in detail. This work confirms that it is possible to obtain wines with very different characteristics from this variety of grapes, producing premium wines with a distinctive pattern of volatiles, reproducible across several vintages and variable depending on the different location of the vineyard and winemaking techniques. Young wines are characterised by fruity, thiolic notes, while wines aged for longer are distinguished by their norisoprenoids content and by anise and balsamic notes, which can be attributed to the presence of 3-MND and to methyl salicylate released by precursors. With a derivatisation GC-MS-MS method it was possible to quantify 3-MND in the range of 10–50 ng L−1. With the use of GC-O, 48 potentially odour-active compounds were found for this wine. Analysis of the compounds after hydrolysis confirmed that a high amount of methyl salicylate characterised this variety. Methyl salicylate has a balsamic note from wintergreen oil that is often perceived in aged Verdicchio tasting.
Avaliação do crescimento inicial de Jatropha curcas L. sob diferentes doses de fósforo aplicados na base
Authors: Arruda Rejanne Lima , Queiroz Poliana Alves de , Costa Neumárcio Vilanova da , Saraiva Althiéris de Souza , Erasmo Eduardo Andrea Lemus
Published: October 2019

This study aimed to analyze the influence of different doses of phosphorus (P2O5) applied at the base on the initial growth of Jatropha curcas L. The experimental randomized blocks design was used with four replications. The treatments consisted of the following doses of phosphorus fertilization: 0, 50, 100, 150 and 200 g plant-1 of P2O5 at 36, 60, 71, 85, 106, 140, 177, 199, 235 and 263 days after seedlings transplanting. Growth parameters evaluated were as follows: plant height, stem diameter, number of primary and secondary branches, number of inflorescences, fruits number, leaf area and seed yield. The P2O5 levels influenced the early growth of the plants. For most of the evaluated characteristics (plant height, number of primary branches, inflorescences, number of fruits), a dose of 150 g plant-1 was the one that promoted greater increase to 140 DAT. Due to the high genetic variability among plants, and the fact that scientists in the field of genetic improvement are working for the development of cultivars with desirable agronomic characteristics, it is necessary to conduct further studies with phosphate fertilizer for the cultivation of Jatropha.
Как сделать тавтологии ясными?
Authors: Боброва Ангелина Сергеевна
Published: October 2019

В статье показывается, каким образом первый раздел теории экзистенциальных графов Ч. Пирса отвечает на вопрос Л. Витгенштейна: «Как должна быть устроена система знаков, чтобы каждая тавтология распознавалась в ней одним и тем же способом?» Теория экзистенциальных графов или теория графов – диаграмматическая логическая система, базовой единицей которой является диаграмма (внешне похожая на диаграммы Эйлера). Ее первый раздел, альфа-графы, примерно соотносится с пропозициональным фрагментом классической логики. Синтаксис теории нагляден, точнее, он иконичен, а потому иконичным оказывается и решение задачи Витгенштейна. Чтобы определить тип формулы, не требуется никаких преобразований. Тавтологии наблюдемы. Возможность усматривать тавтологии объясняется не только диаграмматическими особенностями синтаксиса, но и его минимальностью. Единственным знаком теории (первый раздел) является разрез (контур упомянутой круговой диаграммы): размещение разрезов рядом друг с другом, внутри друг друга порождает не нового вида знаки, а различного вида графы. Разрез выполняет техническую и логическую функции. В этом смысле теория графов оказывается лаконичнее теорий с NAND- или NOR-операторами. В свете рассуждений о тавтологиях в статье затрагивается вопрос эволюции разреза. Разрез, который при самом простом толковании понимается как негация, представляет собой вырожденную импликацию. Именно импликация, а не негация, конъюнкция или дизъюнкция оказывается первичным знаком теории. На первый взгляд такое решение может показаться странным: импликация – самая сложная для понимания логическая операция. Вместе с тем именно импликация подчеркивает фундаментальную роль логического следования, отражает его основные свойства (антисимметричность и транзитивность).
Жан ван Хейеноорт как историк логики
Authors: Бажанов Валентин Александрович
Published: October 2019

В статье предпринимается попытка достаточно лаконичного обзора жизни и творчества Жана ван Хейеноорта (1912–1986) в области истории логики. Приводится информация биографического характера, в которой отмечается отношение ученого к марксизму и его тесное в определенный период сотрудничество с Л.Д. Троцким, разочарование в ключевых принципах марксистской доктрины и ее практической реализации, но сохранение в целом интереса к этому политическому течению. На основании некоторых неопубликованных материалов, полученных из архива американской математики в Остине, штат Техас, описываются работы ван Хейеноорта в области истории логики. Отмечается, что математическое образование он получил как геометр и тополог, но его интересы перенеслись в логику, которая по природе своей доказательности и канонам строгости рассуждений была близка геометрии. Особый акцент в статье делается на обстоятельствах работы над фундаментальной антологией развития логической мысли "От Фреге до Гёделя", составленной и прокомментированной ван Хейеноортом и рядом его коллег. Обращается внимание на то, что вне поля зрения антологии осталось алгебраическое направление развития математической логики. Описываются труды ван Хейеноорта, относящиеся к теореме о неполноте и первым двум томам собрания сочинений К. Гёделя в начале 1980-х годов, прервавшиеся в результате гибели ван Хейеноорта. Приводятся оценки его коллег работ ван Хейеноорта по истории логики.
A clinical scoring system for pediatric hand-foot-mouth disease
Authors: Huang Hui , Deng Li , Jia Liping , Zhu Runan
Published: October 2019

Background:The aim of the present study was to develop a clinical scoring system for the diagnosis of hand-foot-mouth disease (HFMD) with improved accuracy.Methods: A retrospectiveanalysis was performed on standardized patienthistory and clinicalexamination data obtained from 1435 pediatric patients under the age of three years who presented with acute rash illness and underwent enterovirus nucleicaciddetection. Patients were then divided into the HFMD (1094 patients) group or non-HFMD (341 patients) group based on a positive or a negative result from the assay, respectively. We then divided the data into a training set (1004 cases, 70%) and a test set (431 cases, 30%) using a random number method. Multivariate logistic regression was performed on 15 clinical variables (e.g. age, exposure history, number of rash spots in a single body region) to identify variables highly predictive of a positive diagnosisin the training set.Using the variables with high impact on the diagnostic accuracy, we generated a scoring system for predicting HFMDand subsequently evaluated this system in the test set.Results: Using the logistic model, we identified seven clinical variables (age, exposure history,and rash density at specific regions of the body) to be included into the scoring system. The final scores ranged from −5 to 24 (higher scores positively predicted HFMD diagnosis).Through our training set,a cutoff score of 7resulted ina sensitivity of 0.76 and specificity of 0.68. The area under the receiver operating characteristiccurve(AUC)was 0.804 (95% confidence interval [CI]: 0.773–0.835)(P < 0.001). Using the test set, we obtained an AUC of 0.76 (95% CI: 0.710–0.810) with a sensitivity of 0.76 and a specificity of 0.62. These results from the test set were consistent with those from the training set.Conclusions:This study establishes an objective scoring system for the diagnosis of typical and atypical HFMD using measures accessible through routine clinical encounters. Due to the accuracy and sensitivity achieved by this scoring system, it can be employed as a rapid, low-cost method for establishing diagnoses in children with acute rash illness.
Genome Wide Search to Identify Reference Genes candidates for Gene Expression Analysis in Gossypium hirsutum
Authors: K Smitha P , K Vishnupriyan , Kar Ananya S. , M Anil Kumar , Bathula Christopher , N Chandrashekara K , Dhar Sujan K , Das Manjula
Published: October 2019

Background: Cotton is one of the most important commercial crops as the source of natural fiber, oil and fodder. To protect it from harmful pest populations number of newer transgenic lines have been developed. For quick expression checks in successful agriculture qPCR (quantitative polymerase chain reaction) have become extremely popular. The selection of appropriate reference genes plays a critical role in the outcome of such experiments as the method quantifies expression of the target gene in comparison with the reference. Traditionally most commonly used reference genes are the “ house-keeping genes”, involved in basic cellular processes. However, expression levels of such genes often vary in response to experimental conditions, forcing the researchers to validate the reference genes for every experimental platform. This study presents a data science driven unbiased genome-wide search for the selection of reference genes by assessing variation of >50,000 genes in a publicly available RNA- seq dataset of cotton species Gossypium hirsutum .
Result: Five genes ( TMN5, TBL6, UTR5B, AT1g65240 and CYP76B6 ) identified by data-science driven analysis, along with two commonly used reference genes found in literature ( PP2A1 and UBQ14 ) were taken through qPCR in a set of 33 experimental samples consisting of different tissues (leaves, square, stem and root), different stages of leaf (young and mature) and square development (small, medium and large) in both transgenic and non-transgenic plants. Expression stability of the genes was evaluated using four algorithms - geNorm , BestKeeper , NormFinder and RefFinder.
Conclusion: Based on the results we recommend the usage of TMN5 and TBL6 as the optimal candidate reference genes in qPCR experiments with normal and transgenic cotton plant tissues. AT1g65240 and PP2A1 can also be used if expression study includes squares. This study, for the first time successfully displays a data science driven genome-wide search method followed by experimental validation as a method of choice for selection of stable reference genes over the selection based on function alone.
Evaluation of vision-related quality of life with autologous internal limiting membrane transplantation for refractory macular hole.
Authors: yuan dongqing , Zhang Weiwei , Yuan Songtao , Xie Ping , Liu Qinghuai
Published: October 2019

Background: To evaluate the vision-related quality of life of vitrectomy combined with autologous internal limiting membrane(ILM) transplantation for refractory macular hole． Methods: A prospective and nonrandomized clinical study was carried out. There were fourty eyes with refractory macular hole included and all eyes received 23G vitrectomy and ILM peeling with autologous ILM transplantation. Preoperative and postoperative basic conditions were recorded, including best corrected visual acuity (BCVA), intraocular pressure, central retinal thickness (CRT) measurement by Optical coherence tomography (OCT) examination, macular hole index (MHI) and operative complications. The Chinese version of visual-related quality of life scale -25 (CVRQoL-25) was used to evaluate the visual related quality of life of patients after operation. The correlation between the quality of life and the postoperative visual acuity and the size of the macular hole before operation was tested by Spearman rank correlation test. Results: All patients were followed up for three months after surgery, and 38 patients achieved anatomical closure. The mean postoperative logMAR BCVA was 1.09±0.33, which has significantly improved than that before operation (P=0.000). The vision-related quality of life of patients after surgery was closely related to the macular hole index (r=0.375, P=0.017), but was negatively correlated with the best corrected visual acuity before and after surgery (r=-0.495, P=0.001; r=-0.760，P=0.000). It was also found that the vision-related quality of life of patients positively correlated with the postoperative CRT ( r=0.414，P=0.008). Conclusions: The anatomical structure of refractory macular hole patients with ILM peeling combined with autologous ILM transplantation was largely reduced, and the visual acuity of the patients improved significantly. Meanwhile, the vision-related quality of life was significantly improved after surgery. Trial Registration: ChiCTR-INR-16008660, date of registration: 2016/06/17
A meta-analysis of comparison of proximal gastrectomy with double-tract reconstruction and total gastrectomy for proximal early gastric cancer
Authors: Li Shengnan , Gu Lihu , Shen Zefeng , Mao Danyi , Khadaroo Parikshit Asutosh , Su Hui
Published: October 2019

Background In theory, proximal gastrectomy with double-tract reconstruction (PG-DT) was superior to total gastrectomy (TG) in hematologic and nutritional outcomes. However, its clinical effects in proximal early gastric cancer (EGC) have been controversial. Methods The purpose of this study was to investigate the outcomes of laparoscopic proximal gastrectomy with double-tract reconstruction (LPG-DT) for proximal EGC. For this systematic review and meta-analysis, we searched for articles published before December of 2018 in the following databases: PubMed, Web of Science, EBSCO, Medline, and Cochrane Library. Results The results showed no significant difference in the anastomotic stenosis (OR=0.91, 95%CI=0.33-2.50, p=0.85) and reflux esophagitis (OR=1.87, 95%CI=0.62-5.65, p=0.27) between LPG-DT and laparoscopic total gastrectomy (LTG). The vitamin B12 supplementation rate in the LPG-DT group was lower than the LTG group (OR=0.06, 95%Cl=0.01-0.59, p=0.02). Conclusions Due to comparable clinical effect, PG-DT is comparable to TG for patients with proximal EGC. In addition, LPG-DT not only appears superior to TG in terms of preventing vitamin B12 deficiency, but also does not increase the risk of anastomotic stricture and reflux esophagitis.
The effect of vitamin D supplementation on the glycemic control of pre-diabetic Qatari patients in a randomized control trial.
Authors: Thani Mohammed Al , Sadoun Eman , Sofroniou Angeliki , Jayyousi Amin , Baagar Khaled Ahmed Mohamed , Hammaq Abdulla Al , Vinodson Benjamin , Akram Hammad , Bhatti Zaid Shakoor , Nasser Heba Samir , Leventakou Vasiliki
Published: October 2019

Background: Vitamin D deficiency is associated with indicators of pre-diabetes including, insulin resistance, β-cell dysfunction and elevated plasma glucose with controversial findings from current trials. This study aims to investigate the long-term effect of vitamin D on glucose metabolism and insulin sensitivity in pre-diabetic and highly vitamin-deficient subjects. Methods: 132 participants were randomized to 30,000 IU vitamin D weekly for 6 months. Participants underwent oral glucose tolerance test (OGTT) at 3-month intervals to determine the change in plasma glucose concentration at 2h after 75g OGTT (2hPCG). Secondary measurements included glycated hemoglobin, fasting plasma glucose and insulin, post-prandial insulin, indices of insulin sensitivity (HOMA-IR, Matsuda Index), β-cell function (HOMA-β, glucose and insulin area under the curve (AUC), disposition and insulinogenic indices), and lipid profile. Results: A total of 57 (vitamin D) and 75 (placebo) subjects completed the study. Mean baseline serum 25(OH) D levels were 17.0 ng/ml and 14.9 ng/ml for placebo and vitamin D group, respectively. No significant differences were observed for 2hPC glucose or insulin sensitivity indices between groups. HOMA-β significantly decreased in the vitamin D group, while area under curve for glucose and insulin showed a significant reduction in β-cell function in both groups. Additionally, HOMA-β was found to be significantly different between control and treatment group and significance persisted after adjusting for confounding factors. Conclusion: Vitamin D supplementation in a pre-diabetic and severely vitamin-deficient population had no effect on glucose tolerance or insulin sensitivity. The observed reduction in β-cell function in both placebo and vitamin D groups could be attributed to factors other than supplementation. Trial registration: NCT02098980, 28/03/2014 (www.clinicaltrials.gov).
A lil3 chlp double mutant with exclusive accumulation of geranylgeranyl chlorophyll displays a lethal phenotype in rice
Authors: Li Chunmei , Liu Xin , Pan Jihong , Guo Jia , Wang Qian , Chen Congping , Li Na , Zhang Kuan , Yang Bin , Sun Changhui , Deng Xiaojian , Wang Pingrong
Published: October 2019

Background:
Phytyl residues are the common side chains of chlorophyll (Chl) and tocopherols. Geranylgeranyl reductase (GGR), which is encoded by CHLP gene, is responsible for phytyl biosynthesis. The light-harvesting like protein LIL3 was suggested to be required for stability of GGR and protochlorophyllide oxidoreductase in Arabidopsis.
Results:
In this study, we isolated a yellow-green leaf mutant, 637ys , in rice ( Oryza sativa ). The mutant accumulated majority of Chls with unsaturated geranylgeraniol side chains and displayed a yellow-green leaf phenotype through the whole growth period. The development of chloroplasts was suppressed, and the major agronomic traits, especially No. of productive panicles per plant and of spikelets per panicle, dramatically decreased in 637ys . Besides, the mutant exhibited to be sensitive to light intensity and deficiency of tocopherols without obvious alteration in tocotrienols in leaves and grains. Map-based cloning and complementation experiment demonstrated that a point mutation on the OsLIL3 gene accounted for the mutant phenotype of 637ys . OsLIL3 is mainly expressed in green tissues, and its encoded protein is targeted to the chloroplast. Furthermore, the 637ys 502ys ( lil3 chlp ) double mutant exclusively accumulated geranylgeranyl Chl and exhibited lethality at the three-leaf stage.
Conclusions:
We identified the OsLIL3 gene through a map-based cloning approach. Meanwhile, we demonstrated that OsLIL3 is of extreme importance to the function of OsGGR, and that the complete replacement of phytyl side chain of chlorophyll by geranylgeranyl chain could be fatal to plant survival in rice.
Associations of serum low-density lipoprotein and systolic blood pressure levels with type 2 diabetic patients with and without peripheral neuropathy: Systemic review, meta-analysis and meta-regression analysis of observational studies
Authors: Naqvi Syed Shah Zaman Haider , Author) Saber imani(Former Corresponding , Hosseinifard Hossein , Wen QingLian , Shahzad M. Naveed , Ijaz Iqra , Deng Youcai , Guo Man , Author) Yong Xu(New Corresponding
Published: October 2019

Background:Compositional abnormalities in lipoproteins and cardiovascular risk factors play an important role in the progression of diabetic peripheral neuropathy (DPN). This systematic review aimed to estimate the predicting value of low-density lipoprotein (LDL) and systolic blood pressure (SBP) level in type-2 diabetes mellitus (T2DM) patients with and without peripheral neuropathy. We also tried to determine whether LDL and SBP are associated with an increased collision risk of DPN.
Materials and Methods:A systematic search was conducted for eligible publications which explored the LDL and SBP level in T2DM patients with and without peripheral neuropathy. The quality of the included studies was assessed by the QUADAS-2 tool. The standardized mean difference (SMD) with 95% CI of LDL and SBP level were pooled to assess the correlation between LDL and SBP level with DPN. We performed random effects meta-regression analyses to investigate factors associated with an increased collision risk of DPN.
Results:There was a significant association between LDL and SBP with poor prognosis of DPN in those included studies (I2= 88.1% and I2= 84.9%, respectively, Both P < 0.001). European T2DM patients have higher serum level of LDL in compare with the European DPN patients (SMD = 0.16, 95 % CI: -0.06 - 0.38; P <0.001). SBP level was associated with a 2.6-fold decrease in non-DPN patients of T2DM (SMD = -2.63, 95% CI: -4.00 - -1.27, P <0.001). Old age European T2DM patients have significantly high risk for diabetes drivers. Furthermore, the results of the case-control study design model are more precise to show the accuracy of SBP in Asian T2DM patients.
Conclusion:Our finding supports the LDL and SBP status could be associated with increased risk of peripheral neuropathy in T2DM patients.
Nutritional deficiency and associated factors among new pulmonary tuberculosis patients of Bale Zone Hospitals, Southeast Ethiopia
Authors: Hussien Bedru , Hussen Mohammedaman Mama , Seid Abdulwahhab , Hussen Abduljewad
Published: October 2019

Objective
Tuberculosis remains deadliest communicable diseases responsible for ill health among millions of people each year and is associated with malnutrition. Addressing nutritional deficiency in Tuberculosis patients is an important aspect of Tuberculosis control programme. Therefore, the aim was to assess the magnitude of nutritional deficiency and associated factors among new pulmonary tuberculosis patients of Bale Zone Hospitals, South-east Ethiopia, 2018. Cross-sectional study design was utilized. Data collection was carried out using structured questionnaires and anthropometric measurements. Body mass index was calculated to determine nutritional deficiency. Crude and adjusted odds ratios together with their corresponding 95% confidence intervals were computed. P-value < 0.05 was considered to declare a result as statistically associated.
Results
Prevalence of nutritional deficiency was 63.2 %. The mean Body mass index for all enrolled participants was 17.86 kg/m2. Employment status of the patients, p-value 0.012 (AOR = 1.82; 95 % CI = 1.14, 2.89) and Khat chewing, p-value 0.02 (AOR = 0.43; 95 % CI = 0.23, 0.85) were factors independently associated with Nutritional Deficiency. Prevalence of Nutritional Deficiency was found to be high. Nutritional support for the needy, regular nutritional assessment and dietary counseling are necessary for better treatment outcome and effective Tuberculosis control programme.
Protective and Therapeutic Effects of Nanoliposomal Quercetin on Acute Liver Injury in Rats
Authors: Liu Xiangyan , Zhang Yang , Liu Ling , Pan Yifeng , Hu Yu , Yang Pu , liao mingmei
Published: October 2019

Background Quercetin, a pigment (flavonoid) found in many plants and foods, has good effects on protecting liver function but poor solubility and bioavailability in vivo. A drug delivery system can improve the accumulation and bioavailability of quercetin in liver. Objective In this study, we used liposomal nanoparticles to entrap quercetin and evaluated its protective and therapeutic effects on drug-induced liver injury in rats. Design The nanoliposomal quercetin was prepared by a thin film evaporation-high pressure homogenization method and characterized by morphology, particle size and drug content. Acute liver injury was induced in rats by composite factors, including carbon tetrachloride injection, high-fat corn powder intake and ethanol drinking. After pure quercetin or nanoliposomal quercetin treatment, liver function was evaluated by detecting serum levels of glutamic-pyruvic transaminase (GPT), glutamic-oxal acetic transaminase (GOT) and direct bilirubin (DBIL). Histology of injured liver tissues was evaluated by hematoxylin and eosin staining. Results and discussion On histology, liposomal nanoparticles loading quercetin were evenly distributed spherical particles. The nanoliposomal quercetin showed high bioactivity and bioavailability in rat liver and markedly attenuated the liver index and pathologic changes in injured liver tissue. With nanoliposomal quercetin treatment, the serum levels of GPT, GOT and DBIL were significantly better than treated with pure quercetin. Using liposomal nanoparticles to entrap quercetin might be an effective strategy to reduce hepatic injury and protect hepatocytes against damage. Conclusions Liposomal nanoparticles may improve the solubility and bioavailability of quercetin in liver. Furthermore, nanoliposomal quercetin could effectively protect rats against acute liver injury and may be a new hepatoprotective and therapeutic agent for patients with liver diseases.
Gene signature characteristic of elevated stromal infiltration and activation is associated with increased risk of hematogenous and lymphatic metastasis in serous ovarian cancer
Authors: Yue Huiran , Wang Jieyu , Chen Ruifang , Hou Xiaoman , Li Jun , Lu Xin
Published: October 2019

Background The clinical significance of hematogenous and lymphatic metastasis in ovarian cancer has been increasingly addressed, as it plays an imperative role in the formation of both intraperitoneal and distant metastases. Our objective is to identify the key molecules and biological processes potentially related to this relatively novel metastatic route in serous ovarian cancer.Methods Since lymphovascular space invasion (LVSI) is considered as the first step of hematogenous and lymphatic dissemination, we developed a gene signature mainly based on the transcriptome profiles with available information on LVSI status in the Cancer Genome Atlas (TCGA) dataset. We then explored the underlying biological rationale and prognostic value of the identified gene signature using multiple public databases.Results We observe that primary tumors with increased risk of hematogenous and lymphatic metastasis highly express a panel of genes, namely POSTN, LUM, THBS2, COL3A1, COL5A1, COL5A2, FAP1 and FBN1. The identified geneset is characterized by enhanced deposition of extracellular matrix and extensive stromal activation. Mechanistically, both the recruitment and the activation of stromal cells, especially fibroblasts, are closely associated with lymphovascular metastasis. Survival analysis further reveals that the elevated expression of the identified genes correlates to cancer progression and poor prognosis in patients with serous ovarian cancer.Conclusions Our findings indicate that tumor stroma supports the hematogenous and lymphatic spread of ovarian cancer, increasing tumor invasiveness and ultimately resulting in worse survival. Thus stroma-targeted therapies may improve the clinical outcomes in combination with cytoreductive surgery and chemotherapy.
Acupuncture for patients with insomnia disorder protocol for a resting-state functional magnetic resonance imaging study
Authors: Guo Jing , Yu Siyi , Liu Chunhong , Wang Guiling , li Bin
Published: October 2019

Background Insomnia is among the most prevalent sleep-related disorders. Previous researches suggest insomnia disorder is associated with brain hyperarousal state manifested as abnormal regional brain activity and resting-state functional connectivity (rs-FC). Acupuncture is considered to be beneficial to improve sleep quality and modulate hyperarousal state. Its underlying neurobiological bases remain poorly understood. The purpose of the trial is to investigate the effectiveness and potential neural mechanism in acupuncture treatment for insomnia disorder using neuropsychological measurements and resting-state functional magnetic resonance imaging (rs-fMRI). Methods /Design This study is a randomized, patient-assessor-blinded trial. A total of 60 eligible patients with insomnia disorder will be randomized in a ratio of 1:1 to the real acupuncture group and sham acupuncture group. Acupuncture intervention will be administered to all patients three times a week for 4 weeks, followed up for 8 weeks. 30 age- and sex-matched healthy good sleepers will be recruited as healthy control group without any treatment intervention. All participants will undergo neuropsychological and rs-fMRI evaluations. The primary outcome includes the change of Pittsburgh Sleep Quality Index (PSQI) scores. The secondary outcome includes Hyperarousal scale (HAS),rs-fMRI measurements, Fatigue scale-14 (FS-14), Hamilton depression scale (HAMD), Hamilton anxiety scale (HAMA), a sleep diary, and actigraph test. All outcomes will be evaluated at baseline, post-treatment period and follow-up. The main analyses will be carried out on the basis of the intention-to-treat principle. Discussion The results of the trial will contribute to the efficacy and central mechanism of acupuncture treating insomnia disorder.