bluuebunny/crossref_metadata_2025 huggingface dataset:
Core Bacterial Taxa Determine Formation of Forage Yield in Fertilized Soil
Authors: Wang Xiangtao , Zhao Ningning , Li Wencheng , Pu Xin , Xu Peng , Wang Puchang
Published: August 2024

Understanding the roles of core bacterial taxa in forage production is crucial for developing sustainable fertilization practices that enhance the soil bacteria and forage yield. This study aims to investigate the impact of different fertilization regimes on soil bacterial community structure and function, with a particular focus on the role of core bacterial taxa in contributing to soil nutrient content and enhancing forage yield. Field experiments and high-throughput sequencing techniques were used to analyze the soil bacterial community structure and function under various fertilization regimes, including six treatments, control with no amendment (CK), double the standard rate of organic manure (T01), the standard rate of organic manure with nitrogen input equal to T04 (T02), half the standard rate of inorganic fertilizer plus half the standard rate of organic manure (T03), the standard rate of inorganic fertilizer reflecting local practice (T04), and double the standard rate of inorganic fertilizer (T05). The results demonstrated that organic manure treatments, particularly T01, significantly increased the forage yield and the diversity of core bacterial taxa. Core taxa from the Actinomycetota, Alphaproteobacteria, and Gammaproteobacteria classes were crucial in enhancing the soil nutrient content, directly correlating with forage yield. Fertilization significantly influenced functions relating to carbon and nitrogen cycling, with core taxa playing central roles. The diversity of core microbiota and soil nutrient levels were key determinants of forage yield variations across treatments. These findings underscore the critical role of core bacterial taxa in agroecosystem productivity and advocate for their consideration in fertilization strategies to optimize forage yield, supporting the shift towards sustainable agricultural practices.
Nutritional Management of Patients with Fatty Acid Oxidation Disorders
Authors: Peña-Quintana Luis , Correcher-Medina Patricia
Published: August 2024

Treatment of fatty acid oxidation disorders is based on dietary, pharmacological and metabolic decompensation measures. It is essential to provide the patient with sufficient glucose to prevent lipolysis and to avoid the use of fatty acids as fuel as far as possible. Dietary management consists of preventing periods of fasting and restricting fat intake by increasing carbohydrate intake, while maintaining an adequate and uninterrupted caloric intake. In long-chain deficits, long-chain triglyceride restriction should be 10% of total energy, with linoleic acid and linolenic acid intake of 3–4% and 0.5–1% (5/1–10/1 ratio), with medium-chain triglyceride supplementation at 10–25% of total energy (total MCT+LCT ratio = 20–35%). Trihepatnoin is a new therapeutic option with a good safety and efficacy profile. Patients at risk of rhabdomyolysis should ingest MCT or carbohydrates or a combination of both 20 min before exercise. In medium- and short-chain deficits, dietary modifications are not advised (except during exacerbations), with MCT contraindicated and slow sugars recommended 20 min before any significant physical exertion. Parents should be alerted to the need to increase the amount and frequency of carbohydrate intake in stressful situations. The main measure in emergency hospital treatment is the administration of IV glucose. The use of carnitine remains controversial and new therapeutic options are under investigation.
Characteristics of Patients Lost to Follow-up after Bariatric Surgery
Authors: Krietenstein Laura , Koschker Ann-Cathrin , Miras Alexander Dimitri , Kollmann Lars , Gruber Maximilian , Dischinger Ulrich , Haubitz Imme , Fassnacht Martin , Warrings Bodo , Seyfried Florian
Published: August 2024

After bariatric surgery lifelong follow-up is recommended. Evidence of the consequences and reasons for being lost to follow-up (LTFU) is sparse. In this prospective study follow-up data of all patients who underwent bariatric surgery between 2008 and 2017 at a certified obesity centre were investigated. LTFU patients were evaluated through a structured telephone interview. Overall, 573 patients (female/male 70.9%/29.1%), aged 44.1 ± 11.2 years, preoperative BMI 52.1 ± 8.4 kg/m2 underwent bariatric surgery. Out of these, 33.2% had type 2 diabetes mellitus and 74.4% had arterial hypertension. A total of 290 patients were LTFU, of those 82.1% could be reached. Baseline characteristics of patients in follow-up (IFU) and LTFU were comparable, but men were more often LTFU (p = 0.01). Reported postoperative total weight loss (%TWL) and improvements of comorbidities were comparable, but %TWL was higher in patients remaining in follow-up for at least 2 years (p = 0.013). Travel issues were mentioned as the main reason for being LTFU. A percentage of 77.6% of patients reported to regularly supplement micronutrients, while 71.0% stated regular monitoring of their micronutrient status, mostly by primary care physicians. Despite comparable reported outcomes of LTFU to IFU patients, the duration of the in-centre follow-up period affected %TWL. There is a lack of sufficient supplementation and monitoring of micronutrients in a considerable number of LTFU patients.
A Retrospective Study of Genetic Characterization in Suspected Visceral Leishmaniasis Cases in Greece, 2005 to 2020
Authors: Evangelidou Maria , Makka Sofia , Papadogiannaki Ioanna , Koutantou Myrto , Tegos Nikolaos , Mpimpa Anastasia , Patsoula Eleni , Angelakis Emmanouil
Published: August 2024

Leishmania infantum is considered the predominant Leishmania species responsible for visceral leishmaniasis (VL) in Greece but limited molecular-typing-based studies have been performed so far. We retrospectively analyzed data and serum samples collected from 3661 individuals suspected for VL in a sixteen-year period, from 2005 to 2020, to study the seasonality and demographic characteristics of VL cases and to define the L. infantum genotypes circulating in the country. Serum samples were tested with immunofluorescence assay and/or molecular assay. qPCR Leishmania-positive samples were subjected to genotypic analysis based on polymorphisms in 12 microsatellite regions of the internal transcribed spacers (ITSs) 1 and 2. We diagnosed 219 definite (6%, sample with a positive molecular assay and/or antibody titer ≥ 1:400) and 230 probable (6.3%, sample with antibody titer between 1:100 and 1:200) VL cases. Data analysis revealed that amongst VL-definite cases, the age group (≥65) constitutes the most affected factor, since 36.9% of the VL cases belonged to this age group. Amongst the VL definite cases, the most frequently reported symptoms were fever (83%), splenomegaly (49%), and hepatomegaly (40%), but this was not the case for immunocompromised patients that developed non-typical symptoms of leishmaniasis. Although no statistically significant differences in the overall seasonality of VL cases were observed, February and June showed a significantly higher proportion of VL cases compared to August and December. Genotyping of ITS1 and ITS2 regions revealed that all VL cases belong to ITS type A of L. infantum. Our study provides epidemiological information on VL and demonstrates for the first time, providing genotypic data, the circulation of ITS type A L. infantum in Greece.
Large-Scale Serological Survey of Crimean-Congo Hemorrhagic Fever Virus and Rift Valley Fever Virus in Small Ruminants in Senegal
Authors: Gahn Marie Cicille Ba , Diouf Gorgui , Cissé Ndjibouyé , Ciss Mamadou , Bordier Marion , Ndiaye Mbengué , Bakhoum Mame Thierno , Djiba Mamadou Lamine , Brown Corrie , Faburay Bonto , Fall Assane Gueye , Lo Modou Moustapha
Published: August 2024

Crimean-Congo hemorrhagic fever (CCHF) and Rift Valley fever (RVF) are among the list of emerging zoonotic diseases that require special attention and priority. RVF is one of the six priority diseases selected by the Senegalese government. Repeated epidemic episodes and sporadic cases of CCHF and RVF in Senegal motivated this study, involving a national cross-sectional serological survey to assess the distribution of the two diseases in this country throughout the small ruminant population. A total of 2127 sera from small ruminants (goat and sheep) were collected in all regions of Senegal. The overall seroprevalence of CCHF and RVF was 14.1% (IC 95%: 12.5–15.5) and 4.4% (95% CI: 3.5–5.3), respectively. The regions of Saint-Louis (38.4%; 95% CI: 30.4–46.2), Kolda (28.3%; 95% CI: 20.9–35.7), Tambacounda (22.2%; 95% CI: 15.8–28.6) and Kédougou (20.9%; 95% CI: 14.4–27.4) were the most affected areas. The risk factors identified during this study show that the age, species and sex of the animals are key factors in determining exposure to these two viruses. This study confirms the active circulation of CCHF in Senegal and provides important and consistent data that can be used to improve the surveillance strategy of a two-in-one health approach to zoonoses.
Advances in Engineering Circular RNA Vaccines
Authors: Zhang Zhongyan , Fu Yuanlei , Ju Xiaoli , Zhang Furong , Zhang Peng , He Meilin
Published: August 2024

Engineered circular RNAs (circRNAs) are a class of single-stranded RNAs with head-to-tail covalently linked structures that integrate open reading frames (ORFs) and internal ribosome entry sites (IRESs) with the function of coding and expressing proteins. Compared to mRNA vaccines, circRNA vaccines offer a more improved method that is safe, stable, and simple to manufacture. With the rapid revelation of the biological functions of circRNA and the success of Severe Acute Respiratory Coronavirus Type II (SARS-CoV-2) mRNA vaccines, biopharmaceutical companies and researchers around the globe are attempting to develop more stable circRNA vaccines for illness prevention and treatment. Nevertheless, research on circRNA vaccines is still in its infancy, and more work and assessment are needed for their synthesis, delivery, and use. In this review, based on the current understanding of the molecular biological properties and immunotherapeutic mechanisms of circRNA, we summarize the current preparation methods of circRNA vaccines, including design, synthesis, purification, and identification. We discuss their delivery strategies and summarize the challenges facing the clinical application of circRNAs to provide references for circRNA vaccine-related research.
Employing Machine Learning-Based QSAR for Targeting Zika Virus NS3 Protease: Molecular Insights and Inhibitor Discovery
Authors: Altayb Hisham N. , Alatawi Hanan Ali
Published: August 2024

Zika virus infection is a mosquito-borne viral disease that has become a global health concern recently. Zika virus belongs to the Flavivirus genus and is primarily transmitted by Aedes mosquitoes. Prevention of Zika virus infection involves avoiding mosquito bites by using repellent, wearing protective clothing, and staying in screened areas, especially for pregnant women. Treatment focuses on managing symptoms with rest, fluids, and acetaminophen, with close monitoring for pregnant women. Currently, there is no specific antiviral treatment or vaccine for the Zika virus, highlighting the importance of prevention strategies to control its spread. Therefore, in this study, the Zika virus non-structural protein NS3 was targeted to inhibit Zika infection by identifying the novel inhibitor through an in silico approach. Here, 2864 natural compounds were screened using a machine learning-based QSAR model, and later docking was performed to select the potential target. Subsequently, Tanimoto similarity and clustering were performed to obtain the potential target. The three most potential compounds were obtained: (a) 5297, (b) 432449, and (c) 85137543. The protein–ligand complex’s stability and flexibility were then investigated by dynamic modelling. The 300 ns simulation showed that 5297 exhibited the steadiest deviation and constant creation of hydrogen bonds. Compared to the other compounds, 5297 demonstrated a superior binding free energy (ΔG = −20.81 kcal/mol) with the protein when the MM/GBSA technique was used. The study determined that 5297 showed significant therapeutic potential and justifies further experimental investigation as a possible inhibitor of the NS2B-NS3 protease target implicated in Zika virus infection.
Advances in Intrathecal Nanoparticle Delivery: Targeting the Blood–Cerebrospinal Fluid Barrier for Enhanced CNS Drug Delivery
Authors: Madadi Ahmad Khalid , Sohn Moon-Jun
Published: August 2024

The blood–cerebrospinal fluid barrier (BCSFB) tightly regulates molecular exchanges between the bloodstream and cerebrospinal fluid (CSF), creating challenges for effective central nervous system (CNS) drug delivery. This review assesses intrathecal (IT) nanoparticle (NP) delivery systems that aim to enhance drug delivery by circumventing the BCSFB, complementing approaches that target the blood–brain barrier (BBB). Active pharmaceutical ingredients (APIs) face hurdles like restricted CNS distribution and rapid clearance, which diminish the efficacy of IT therapies. NPs can be engineered to extend drug circulation times, improve CNS penetration, and facilitate sustained release. This review discusses key pharmacokinetic (PK) parameters essential for the effectiveness of these systems. NPs can quickly traverse the subarachnoid space and remain within the leptomeninges for extended periods, often exceeding three weeks. Some designs enable deeper brain parenchyma penetration. Approximately 80% of NPs in the CSF are cleared through the perivascular glymphatic pathway, with microglia-mediated transport significantly contributing to their paravascular clearance. This review synthesizes recent progress in IT-NP delivery across the BCSFB, highlighting critical findings, ongoing challenges, and the therapeutic potential of surface modifications and targeted delivery strategies.
Searching for Novel HDAC6/Hsp90 Dual Inhibitors with Anti-Prostate Cancer Activity: In Silico Screening and In Vitro Evaluation
Authors: Pinzi Luca , Belluti Silvia , Piccinini Isabella , Imbriano Carol , Rastelli Giulio
Published: August 2024

Prostate cancer (PCA) is one of the most prevalent types of male cancers. While current treatments for early-stage PCA are available, their efficacy is limited in advanced PCA, mainly due to drug resistance or low efficacy. In this context, novel valuable therapeutic opportunities may arise from the combined inhibition of histone deacetylase 6 (HDAC6) and heat shock protein 90 (Hsp90). These targets are mutually involved in the regulation of several processes in cancer cells, and their inhibition is demonstrated to provide synergistic effects against PCA. On these premises, we performed an extensive in silico virtual screening campaign on commercial compounds in search of dual inhibitors of HDAC6 and Hsp90. In vitro tests against recombinant enzymes and PCA cells with different levels of aggressiveness allowed the identification of a subset of compounds with inhibitory activity against HDAC6 and antiproliferative effects towards LNCaP and PC-3 cells. None of the candidates showed appreciable Hsp90 inhibition. However, the discovered compounds have low molecular weight and a chemical structure similar to that of potent Hsp90 blockers. This provides an opportunity for structural and medicinal chemistry optimization in order to obtain HDAC6/Hsp90 dual modulators with antiproliferative effects against prostate cancer. These findings were discussed in detail in the study.
Unlocking Cholesterol Metabolism in Metabolic-Associated Steatotic Liver Disease: Molecular Targets and Natural Product Interventions
Authors: Li Xiaoxiao , Li Meng
Published: August 2024

Metabolic-associated steatotic liver disease (MASLD), the hepatic manifestation of metabolic syndrome, represents a growing global health concern. The intricate pathogenesis of MASLD, driven by genetic, metabolic, epigenetic, and environmental factors, leads to considerable clinical variability. Dysregulation of hepatic lipid metabolism, particularly cholesterol homeostasis, is a critical factor in the progression of MASLD and its more severe form, metabolic dysfunction-associated steatohepatitis (MASH). This review elucidates the multifaceted roles of cholesterol metabolism in MASLD, focusing on its absorption, transportation, biosynthesis, efflux, and conversion. We highlight recent advancements in understanding these processes and explore the therapeutic potential of natural products such as curcumin, berberine, and resveratrol in modulating cholesterol metabolism. By targeting key molecular pathways, these natural products offer promising strategies for MASLD management. Finally, this review also covers the clinical studies of natural products in MASLD, providing new insights for future research and clinical applications.
Integrated Effects of Soil Moisture on Wheat Hydraulic Properties and Stomatal Regulation
Authors: Wang Lijuan , Zhang Yanqun , Luo Dandan , Hu Xinlong , Feng Pancen , Mo Yan , Li Hao , Gong Shihong
Published: August 2024

The development of water-saving management relies on understanding the physiological response of crops to soil drought. The coordinated regulation of hydraulics and stomatal conductance in plant water relations has steadily received attention. However, research focusing on grain crops, such as winter wheat, remains limited. In this study, three soil water supply treatments, including high (H), moderate (M), and low (L) soil water contents, were conducted with potted winter wheat. Leaf water potential (Ψleaf), leaf hydraulic conductance (Kleaf), and stomatal conductance (gs), as well as leaf biochemical parameters and stomatal traits were measured. Results showed that, compared to H, predawn leaf water potential (ΨPD) significantly reduced by 48.10% and 47.91%, midday leaf water potential (ΨMD) reduced by 40.71% and 43.20%, Kleaf reduced by 64.80% and 65.61%, and gs reduced by 21.20% and 43.41%, respectively, under M and L conditions. Although gs showed a significant difference between M and L, Ψleaf and Kleaf did not show significant differences between these treatments. The maximum carboxylation rate (Vcmax) and maximum electron transfer rate (Jmax) under L significantly decreased by 23.11% and 28.10%, stomatal density (SD) and stomatal pore area index (SPI) under L on the abaxial side increased by 59.80% and 52.30%, respectively, compared to H. The leaf water potential at 50% hydraulic conduction loss (P50) under L was not significantly reduced. The gs was positively correlated with ΨMD and Kleaf, but it was negatively correlated with abscisic acid (ABA) and SD. A threshold relationship between gs and Kleaf was observed, with rapid and linear reduction in gs occurring only when Kleaf fell below 8.70 mmol m−2 s−1 MPa−1. Our findings demonstrate that wheat leaves adapt stomatal regulation strategies from anisohydric to isohydric in response to reduced soil water content. These results enrich the theory of trade-offs between the carbon assimilation and hydraulic safety in crops and also provide a theoretical basis for water management practices based on stomatal regulation strategies under varying soil water conditions.
Stigma and Glume Characteristics Synergistically Determine the Stigma Exsertion Rate in Thermo-Photo-Sensitive Genic Male Sterile Wheat
Authors: Li Hongsheng , Yang Zhonghui , Li Shaoxiang , Elfanah Ahmed M. S. , Abdelkhalik Sedhom , Tang Xiong , Yin Jian , Ding Mingliang , Liu Kun , Yang Mujun , Wang Xiue
Published: August 2024

Wheat hybrids have been widely demonstrated to have remarkable heterosis or hybrid vigor in increasing yield potential and stability since the 1960s. Two-line hybrid wheat can achieve higher yields than local varieties, especially in marginal environments. However, the commercial application of hybrid wheat is hindered by higher seed costs, primarily due to lower yields in hybrid seed production. Stigma exsertion has been verified as a decisive factor in increasing rice’s hybrid seed yield, but more investigation is needed in hybrid wheat breeding and production. In this study, four thermo-photo-sensitive genic male sterile lines, including K41S, K64S, K66S, and K68S, with different stigma exsertion rates (SERs) were used to compare the differences in floral architecture relative to stigma exsertion over two growing seasons. The results revealed that the K41S and K64S exhibited a relatively higher SER at 21.87% and 22.81%, respectively. No exserted stigma was observed in K66S, and K68S had an SER of only 0.82%. This study found that the stigma length, glume width and the length–width ratio of the glume were significantly correlated with the SER, with correlation coefficients of 0.46, −0.46 and 0.60, respectively. Other stigma features such as the branch angle, stretch width and hairbrush length, as well as the glume length, also had a weakly positive correlation with SER (r = 0.09–0.27). For K41S and K64S, the SER was significantly affected by the differences in the stigma branch angle and stigma stretch width among florets. A cross-pollination survey showed that the out-crossing ability of florets with an exserted stigma was about three times as high as that of florets with a non-exserted stigma. As a result, the stigma-exserted florets that accounted for 21.87% and 22.81% of the total florets in K41S and K64S produced 46.80% and 48.53% of the total cross-pollinated seeds in both sterile lines. These findings suggest that a longer stigma combined with a slender glume appears to be the essential floral feature of stigma exsertion in sterile wheat lines. It is expected that breeding and utilizing sterile lines with a higher SER would be a promising solution to cost-effective hybrid wheat seed production.
The Effects of Soil Acidity and Aluminium on the Root Systems and Shoot Growth of Lotus pedunculatus and Lupinus polyphyllus
Authors: Bell Lucy E. , Moir Jim L. , Black Alistair D.
Published: August 2024

Lotus pedunculatus (lotus) and Lupinus polyphyllus (Russell lupin) persist in the upland grasslands of New Zealand, where soil acidity and associated aluminium (Al) toxicity impede conventional pasture legumes. This experiment investigated the response of lotus and Russell lupin to soil acidity and Al. The species were sown in 20 cm tall 1.2 L pots of acidic upland soil. A mass of 4.5 or 6.7 g lime (CaCO3)/L was added to either the top or bottom or both soil horizons (0–9 cm and 9–18 cm), resulting in six treatments across six randomised blocks in a glasshouse. The soil pH was 4.4, 4.9, and 5.4; the exchangeable Al concentrations were 24, 2.5, and 1.5 mg/kg for 0, 4.5, and 6.7 g lime/L. At 16 weeks post-sowing, the plants were divided into shoots and roots at 0–9 cm and 9–18 cm. Root morphology, shoot and root dry matter (DM), shoot nitrogen (N), and nodulation were measured. The total plant DM and shoot-to-root DM ratio were higher, and the shoot %N was lower for the lotus plants than the Russell lupin plants for the various lime rates (13.2 vs. 2.9 g plant−1, 5.6 vs. 1.6, and 2.4 vs. 3.3%, p < 0.05). No response to lime in terms of total DM or total root morphology parameters was exhibited in either species (p > 0.05). Root morphology adjustments in response to acidity between soil horizons were not observed. The results indicated that lotus and Russell lupin are tolerant to high soil acidity (pH 4.4–5.4) and exchangeable Al (1.5–24 mg kg−1), highlighting their considerable adaptation to grasslands with acidic soils.
Regionwide and Nationwide Floristic Richness Reveal Vascular Plant Diversity in Central Asia
Authors: Ma Suliya , Li Wenjun , Tojibaev Komiljon Sh. , Turginov Orzimat , Yang Weikang , Ma Keping
Published: August 2024

Central Asia (CA) is located in the interior of the Eurasian continent and consists of five countries—Kazakhstan, Kyrgyzstan, Tajikistan, Turkmenistan, and Uzbekistan. It contains the largest concentration of temperate deserts and mountains of CA biodiversity hotspots. However, regionwide floristic diversity is sorely lacking, and nationwide floristic diversity is seriously outdated in this region. Using the data collected by the Mapping Asia Plants (MAP) project, we describe and analyze the diverse floristic characteristics of plant diversity in CA at both the regional and national levels, including the dominant families and genera, endemic taxa, and floristic similarity. The results allow the compilation of a new checklist of vascular plants in CA, including 9643 taxa (1198 genera within 139 families) and 3409 endemic taxa (414 genera in 66 families). We confirm that there are 5695, 4036, 4542, 3005, and 4222 species of vascular plants within the CA countries, of which 532, 326, 505, 175, and 301 species are endemic taxa in Kazakhstan, Kyrgyzstan, Tajikistan, Turkmenistan, and Uzbekistan, respectively. The region’s biodiversity is notable for its high degree of endemism—up to 35.35%—which contributes to the floristic uniqueness and the irreplaceability of CA. Tajikistan, encompassing the most dominant area of the CA mountains, has the highest species density (3.19/100 km2) and endemism (11.12%) among the five countries. Neighboring countries such as Tajikistan and Uzbekistan, and Kazakhstan and Kyrgyzstan share more species in common, while Turkmenistan has less species overlap with the other four countries. Trends in endemic and total taxa are consistent. This comprehensive inventory is novel, revealing CA’s plant diversity in two dimensions and providing a solid foundation for subsequent research that will be beneficial to the transboundary conservation and sustainable use of plant resources in CA.
Green Synthesis of Yttrium Derivatives Nanoparticles Using Pine Needle Leaf Extract: Characterization, Docking, Antibacterial, and Antioxidant Potencies
Authors: Darwich Nourhane A. , Mezher Malak , Abdallah Alaa M. , El-Sayed Ahmed F. , El Hajj Rana , Hamdalla Taymour A. , Khalil Mahmoud I.
Published: August 2024

Green nanoparticles are synthesized using environmentally friendly methods, and natural materials hold significant importance. This makes the process environmentally sustainable and reduces the production of harmful waste by-products. Green nanoparticles exhibit reduced toxicity which is crucial for biomedical applications. The current study suggested that yttrium nanoparticles (YNPs) should be synthesized, characterized, and evaluated for their diverse biological applications due to the rise in antibacterial resistance. The YNPs were prepared using a pine needle leaf extract (PNLE). The structural and morphological features have been investigated using X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), photoluminescence (PL), Fourier transform infrared spectroscopy (FTIR), ultraviolet–visible spectroscopy (UV–vis), and vibrating sample magnetometry (VSM). The XRD pattern demonstrated the presence of yttrium oxide and yttrium nitrate phases. The crystallite size and particle size of the synthesized YNPs measured 1.696 nm and 24.55 nm, respectively. The XPS peaks showed two components with binding energies at 530.940 eV and 532.18 eV due to the bond between O–Y and OH–Y, respectively. Additionally, the ferromagnetic nature of the YNPs was confirmed by VSM analysis. The YNPs were tested for antibacterial activity on six uropathogenic bacteria (S. aureus, S. haemolyticus, E. faecalis, E. coli, K. pneumonia, and P. aeruginosa) using the microdilution assays, to find the minimum inhibitory concentration (MIC) as well as the minimum bactericidal concentration (MBC), the agar well diffusion assay, and antibiofilm screening assays, where they showed bacteriostatic action against all isolates (0.5–1 mg/mL MIC) and significant inhibition of biofilm formation (80% inhibition rate). The antioxidant capacity assessed by 1,1, diphenyl-2-picrylhydrazyl (DPPH) radical scavenging revealed 50% DPPH scavenging. Moreover, docking studies exhibited that YNPs inhibit crucial bacterial enzymes, including DNA gyrase, penicillin-binding proteins, carbapenemase, LasR-binding protein, and dihydropteroate synthase. These findings may explain the mechanisms responsible for the observed antibacterial effects of YNPs. Overall, these findings underscore YNPs as promising candidates for antioxidant and antibacterial applications.
First Release of the Optimal Cloud Analysis Climate Data Record from the EUMETSAT SEVIRI Measurements 2004–2019
Authors: Bozzo Alessio , Doutriaux-Boucher Marie , Jackson John , Spezzi Loredana , Lattanzio Alessio , Watts Philip D.
Published: August 2024

Clouds are key to understanding the atmosphere and climate, and a long series of satellite observations provide invaluable information to study their properties. EUMETSAT has published Release 1 of the Optimal Cloud Analysis (OCA) Climate Data Record (CDR), which provides a homogeneous time series of cloud properties of up to two overlapping layers, together with uncertainties. The OCA product is derived using the 15 min Spinning Enhanced Visible and Infrared Imager (SEVIRI) measurements onboard Meteosat Second Generation (MSG) in geostationary orbit and covers the period from 19 January 2004 until 31 August 2019. This paper presents the validation of the OCA cloud-top pressure (CTP) against independent lidar-based estimates and the quality assessment of the cloud optical thickness (COT) and cloud particle effective radius (CRE) against a combination of products from satellite-based active and passive instruments. The OCA CTP is in good agreement with the CTP sensed by lidar for low thick liquid clouds and substantially below in the case of high ice clouds, in agreement with previous studies. The retrievals of COT and CRE are more reliable when constrained by solar channels and are consistent with other retrievals from passive imagers. The resulting cloud properties are stable and homogeneous over the whole period when compared against similar CDRs from passive instruments. For CTP, the OCA CDR and the near-real-time OCA products are consistent, allowing for the use of OCA near-real time products to extend the CDR beyond August 2019.
A Novel Intelligent Fault Diagnosis Method for Bearings with Multi-Source Data and Improved GASA
Authors: Hu Qingming , Fu Xinjie , Guan Yanqi , Wu Qingtao , Liu Shang
Published: August 2024

In recent years, single-source-data-based deep learning methods have made considerable strides in the field of fault diagnosis. Nevertheless, the extraction of useful information from multi-source data remains a challenge. In this paper, we propose a novel approach called the Genetic Simulated Annealing Optimization (GASA) method with a multi-source data convolutional neural network (MSCNN) for the fault diagnosis of rolling bearing. This method aims to identify bearing faults more accurately and make full use of multi-source data. Initially, the bearing vibration signal is transformed into a time–frequency graph using the continuous wavelet transform (CWT) and the signal is integrated with the motor current signal and fed into the network model. Then, a GASA-MSCNN fault diagnosis method is established to better capture the crucial information within the signal and identify various bearing health conditions. Finally, a rolling bearing dataset under different noisy environments is employed to validate the robustness of the proposed model. The experimental results demonstrate that the proposed method is capable of accurately identifying various types of rolling bearing faults, with an accuracy rate reaching up to 98% or higher even in variable noise environments. The experiments reveal that the new method significantly improves fault detection accuracy.
The Gaussian Plume Model Equation for Atmospheric Dispersion Corrected for Multiple Reflections at Parallel Boundaries: A Mathematical Rewriting of the Model and Some Numerical Testing
Authors: Micallef Alfred , Micallef Christopher
Published: August 2024

The well-known Gaussian plume model has proven to be very useful in simulating the atmospheric dispersion of air pollutants (both gaseous and particulates). Nevertheless, the nature of the model presents problems in the actual computation of concentrations when the plume is confined between two parallel boundaries due to the occurrence of multiple reflections. The ground and temperature inversion lid (especially, when the inversion layer is at low levels in the atmosphere) with a chimney stack releasing the effluent below the latter, is one contextual example of horizontal parallel reflecting boundaries. A second example is buildings confining a roadway on either side, with motor vehicles emitting pollution within the street canyon (or urban notch). In such cases, multiple reflections should be accounted for, otherwise the model underpredicts the resulting concentration. This paper presents a mathematical rewriting of the Gaussian plume model equation corrected for multiple reflections when the pollution source is confined between parallel boundaries. The obtained result is most appropriate when the parallel boundaries are rigid, and near-complete reflection is achieved, e.g., street canyon environment (second quoted example). It is worth noting that the relevant mathematical derivations and definitions are all included in the paper to facilitate reading and to ensure comprehensiveness in the presentation. Additionally, the outcome of some preliminary numerical testing is presented. The latter indicates that the new formulation is mathematically stable and yields interesting results. Further numerical investigation and experimental evaluation are merited.
Technoeconomic Assessment of Phosphoric Acid and Rare Earth Element Recovery from Phosphoric Acid Sludge
Authors: Jang Gyoung Gug , Thompson Joshua A. , Meyer Pimphan Aye , Zhang Patrick , Shen Ziheng , Tsouris Costas
Published: August 2024

Sustainability faces many challenges, including the availability of materials necessary for technological advancement. Rare earth elements (REEs), for example, are key materials for several manufacturing industries that can unlock renewable energy and sustainable development. In this study, a decanter centrifuge has been employed to successfully separated phosphoric acid and REE-containing particles from phosphoric acid sludge with concentrations ranging from 1000 to 2200 ppm REEs. Operating efficiently with up to 35 wt.% solids, the centrifuge was demonstrated to achieve approximately 95% phosphoric acid recovery and 90% REE recovery in a single pass, eliminating the need for additional processing steps. This breakthrough supports a proposed rare earth oxide (REO) recovery process integrating phosphoric acid (PA), elemental phosphorus (P4), and REO into two potential pathways: PA-REO and PA-P4-REO. These processes aim to reintroduce recovered phosphoric acid into the main product to significantly increase output and revenue. Post-separation, phosphorus-rich particles can be converted to P4, while REE-containing solids undergo further treatment including acid leaching, extraction/stripping, precipitation, and calcination to produce a marketable REO material. Technoeconomic analysis indicates promising profitability, with the PA-REO process showing a delta net present value (∆NPV) of USD 441.8 million over a 12-year period and expected return within a year of construction, while the PA-P4-REO process yields a ∆NPV of USD 178.7 million over a 12-year return period. Both pathways offer robust financial prospects and demonstrate the feasibility of commercial-scale REO recovery from phosphoric acid sludge, offering an economically feasible approach to produce REEs for future sustainable development challenges related to sustainability.
Enhancing Forest Conservation Efforts: Mapping of High Conservation Value Forests in the Republic of Moldova
Authors: Talpă Nicolae , Lozan Aurel , Hălălișan Aureliu-Florin , Sfeclă Victor , Popa Bogdan
Published: August 2024

The research aims to identify potential High Conservation Value Forests (HCVFs) in Moldova, seen as crucial for biodiversity and providing long-term benefits. Despite the fact that forests only cover 11% of the Moldova’s territory, they account for more than 80% of the country’s biological diversity and have social, economic, climate, hydrological, and erosion-control benefits. The methodology involved mainly developing identification criteria of these forests, according to the approach developed by FSC, and processing the existing cartographic data using GIS techniques. The findings reveal that almost one-half of Moldova’s forests have potential for HCVFs: approximately 175,500 ha of forest land, accounting for 47.3% of the total forested area, were identified as potential HCVFs. Most of these forests are owned and managed by the state. An important outcome of this research is to contribute to the protection of valuable forest ecosystems and establish discussion platforms with stakeholders for their future conservation and long-term resource management. Forest managers, landscape planners, and beneficiaries of forest ecosystem services can use the findings to align management plans, prioritize conservation, and promote sustainable land use. Proper implementation of HCVF principles can attract donors and investors to support Moldova’s forestry sector.
Does “Dual Credit Policy” Really Matter in Corporate Competitiveness?
Authors: Liang Liang , Mei Qian , Li Chengjiang
Published: August 2024

Developing the new energy vehicle (NEV) industry significantly reduces pollutant emissions in the transportation sector, promotes high-quality carbon peaks, and reduces dependence on oil imports. Industrial policies also support the NEV industry, constantly enhancing its international competitiveness. The Dual Credit Policy, implemented in 2017, has pressured automotive manufacturers to transform their production models, reduce the output of traditional fuel vehicles, and increase the production of NEV. This study analyzes the effects of the Dual Credit Policy on corporate competitiveness before and after implementing it, using listed companies in China’s NEV industry as the research subjects. The results indicate that the Dual Credit Policy significantly enhanced corporate competitiveness through substantial innovation. Additionally, the difference-in-differences (DID) model results reveal that the policy’s promotional effect is more pronounced in traditional vehicle companies due to higher pressure. Heterogeneity tests show that the policy has a more significant effect on state-owned enterprises and that regional marketization differences lead to a greater promotional impact on enterprises in the central and eastern regions.
The Role of Campus Green Space for Residents: Based on Supply–Demand of Recreation Services
Authors: Zhang Zhengkai , Hao Manyuan , Mao Yuyang , Chen Siwei
Published: August 2024

The campus is an important place for recreational activities among surrounding residents, which can alleviate the shortage of urban public green space (PGS). However, evidence is lacking on how much campus green space is complementary to the supply and demand of PGS. For this purpose, we chose Yangling, a university town in western China, as the study area. We compared perceived accessibility and the coupling coordination degree (CCD) of the supply and demand of PGSs for residents with different purposes before and after the COVID-19 pandemic, when the campuses were closed or reopened. After the campus reopened, the number of blocks that were able to reach their most frequently visited green spaces within 5 min had increased from zero to one (walking) and two to four (by bicycle). The proportion of blocks with a high level of CCD had increased from 28.6% to 42.9%. The reopening of campuses can significantly increase the perceived accessibility of PGSs within 5 min and 15 min by walking and bicycle, respectively, in central urban communities, but it was not significant in suburban communities. It also effectively improved the PGS supply of the block where it is located. For residents who aim to exercise, walk, go sightseeing, and communicate, its role was similar overall. For residents who aim to play with children, the campus green spaces could not bring significant improvements. In order to enhance the complementary role of green space on campus, it is important to improve its accessibility for adjacent blocks, increase inside footpath density, and add child-friendly facilities.
Rural Industry Revitalization Can Be Energized by Land Transfer: A Case Study in Guangxi Zhuang Autonomous Region, China, 2013–2022
Authors: Yu Yaqun , Lang Tao , Pan Min , Dai Qiming , Liu Youshun , Huang Yanjing , Lu Xueming , Sun Luyi , Wang Ziyou
Published: August 2024

The Chinese government has vigorously promoted the transfer of land use rights, aiming to promote the scale, intensification, and efficiency of agricultural operations and achieve rural industry revitalization (RIR). However, whether and how land transfer energizes RIR remains unclear. Taking five representative cities (Nanning, Guigang, Baise, Fangchenggang, and Guilin) in Guangxi Zhuang Autonomous Region as a case study, we quantificationally characterized RIR during 2013–2022 using eleven variables and land transfer using three difference indices. We evaluated the contribution of land transfer to RIR and compared it among cities. Results showed that all five cities in Guangxi displayed an increasing trend of land transfer and RIR over the last decade. The increase in RIR was largely contributed by the improvement in infrastructure construction and industry convergence, and, to a lesser extent, by the enhancement in agricultural production efficiency. There was a strong city-specific correlation between RIR and the land transfer indices, indicating a beneficial role of land transfer in RIR. Structural equation modeling further indicated that land transfer promoted RIR, likely through facilitating infrastructure construction, enhancing industry convergence, and improving agricultural production efficiency. These results provide strong evidence that the transfer of land use rights can promote RIR and practical reference for advancing farmers’ well-being and the overall goal of rural revitalization in the future. The framework and the methodology proposed here are applicable elsewhere in China and other countries where scattering allocation of land resources represents a key limitation to agricultural production and economic development.
Research on a Low-Carbon Optimization Strategy for Regional Power Grids Considering a Dual Demand Response of Electricity and Carbon
Authors: Ma Famei , Ying Liming , Cui Xue , Yu Qiang
Published: August 2024

Considering the characteristics of the power system, where “the source moves with the load”, the load side is primarily responsible for the carbon emissions of the regional power grid. Consequently, users’ electricity consumption behavior has a significant impact on system carbon emissions. Therefore, this paper proposes a multi-objective bi-level optimization strategy for source-load coordination, considering dual demand responses for both electricity and carbon. The upper layer establishes a multi-objective low-carbon economic dispatch model for power grid operators, aiming to minimize the system’s total operating cost, the total direct carbon emissions of the power grid, and the disparity in regional carbon emissions. In the lower layer, a low-carbon economic dispatch model for load aggregators is established to minimize the total cost for load aggregators. To obtain the dynamic carbon emission factor signal, a complex power flow tracking method that considers the power supply path is proposed, and a carbon flow tracking model is established. NSGA-II is used to obtain the Pareto optimal frontier set for the upper model, and the ‘optimal’ scheme is determined based on the fuzzy satisfaction decision. The example analysis demonstrates that the interactive carbon reduction effect under the guidance of dual signals is the most effective. This approach fully exploits the carbon reduction potential of the flexible load, enhancing both the economic efficiency and low-carbon operation of the system.
Sustainable Management Practices of State-Owned Forest Farms in Subtropical Regions: Case Study of S Forest Farm in China
Authors: Zhu Lili , Miao Fengtao
Published: August 2024

The construction quality of state-owned forest farms is crucial to the overall development of ecological civilization. Evaluating the development status of these forest farms is essential. Based on field research and existing studies, this paper uses the S (S is a Mandarin Pinyin abbreviation for the name of the forest farm, to maintain anonymity) state-owned forest farm in Fujian Province, a subtropical region, as a case study. The Delphi method and the analytic hierarchy process (AHP) are employed to determine the weight values of various standard indicators. Compared to previous research, this study finds that the sustainability of human resources and socioeconomic factors in forest farms also play a significant role in achieving sustainable management of state-owned forest farms. By collecting indicator values and reference values, an evaluation of the sustainable management status of the S state-owned forest farm is conducted, revealing that its sustainable management status is at a “good” level. However, there are still problems in forest classification, the ratio of plantation to natural forest, pest outbreaks, mechanization of forest production, employee incentives, tourism, and forest certification, which constrain sustainable management. This study proposes strategies to optimize sustainable management from forest production operations and management perspectives.
Mapping Circular Economy in Portuguese SMEs
Authors: Carreira Rui Jorge , Ferreira José Vasconcelos , Ramos Ana Luísa
Published: August 2024

The transition from a linear to a circular economy is an increasingly popular solution to the dual problems of scarcity of virgin resources at the point of extraction and the exponential growth of waste at the point of disposal. The linear economy adheres to a model wherein natural resources are extracted, transformed into products, utilized, and ultimately discarded as waste. In contrast, the circular economy is designed to promote sustainability. This is achieved by reducing the use of raw materials, waste, reuse, recycling, and recovery of materials at the end of the useful life of products. The result is a closed and regenerative cycle that preserves natural resources and minimizes environmental impacts. The manner in which each country and company responds to this reality is markedly disparate. This research endeavors to ascertain the strategies employed by Portuguese SMEs in addressing this challenge. A review of the literature was conducted to ascertain what has been published on this subject. However, the results of this review indicate that the existing literature does not fully address the intended topic. The articles identified in the literature review only partially address this issue, focus on other geographical regions, or in some cases, are dedicated to specific business sectors. In light of the dearth of research on this topic, we turned to the Portuguese context, employing the Grounded Theory methodology to conduct semi-structured interviews with 34 SMEs. Upon analysis of the results, it became evident that the circular economy is not yet a prerequisite for consumer choice. Portuguese legislation does not facilitate the implementation of the circular economy, consumers are not yet engaged with the issue itself, companies face challenges in communicating their practices, and there is a perceived lack of credibility by consumers. Additionally, the cost–benefit ratio is not always favorable for such practices.