bluuebunny/crossref_metadata_2025 huggingface dataset:
Urinary angiotensin-converting enzyme 2 and its activity in cats with chronic kidney disease
Authors: Kuo Tzu-Chien , Hsu Wei-Li , Wu Vin-Cent , Jan Tong-Rong , Tsai Pei-Shiue Jason , Lee Ya-Jane
Published: May 2024

Introduction

Angiotensin-converting enzyme 2 (ACE2) played an important role in the renin-angiotensin-aldosterone system (RAAS) and it was proved to be renoprotective in renal disease. Urinary angiotensin-converting enzyme 2 (uACE2) has been shown to reflect renal injury in human and experimental studies, but its role in feline kidney disease remains unknown.

Aims

Our objectives involve comparing uACE2 concentrations and activities in cats across CKD stages with healthy controls, investigating the relationship between uACE2 concentrations, activities, and clinicopathological data in feline CKD patients, and assessing the predictive abilities of both for CKD progression.

Methods

A retrospective, case–control study. The concentration and activity of uACE2 were measured by commercial ELISA and fluorometric assay kits, respectively. The concentration was adjusted to give uACE2 concentration-to-creatinine ratios (UACCRs).

Results

In total, 67 cats consisting of 24 control and 43 chronic kidney disease (CKD), including 24 early-stage CKD and 19 late-stage CKD, were enrolled in this study. UACCR values were significantly higher in both early-stage (2.100 [1.142–4.242] x 10−6) and late-stage feline CKD (4.343 [2.992–5.0.71] x 10−6) compared to healthy controls (0.894 [0.610–1.076] x 10−6; p < 0.001), and there was also significant difference between-early stage group and late-stage group (p = 0.026). Urinary ACE2 activity (UAA) was significantly lower in CKD cats (1.338 [0.644–2.755] x pmol/min/ml) compared to the healthy cats (7.989 [3.711–15.903] x pmol/min/ml; p < 0.001). UACCR demonstrated an independent, positive correlation with BUN (p < 0.001), and UAA exhibited an independent, negative correlation with plasma creatinine (p < 0.001). Both UACCR and UAA did not yield significant results in predicting CKD progression based on the ROC curve analysis.

Conclusion and clinical importance

uACE2 concentration and activity exhibit varying changes as renal function declines, particularly in advanced CKD cats.
Neonatal vitamin A supplementation improves sheep fertility potential
Authors: Li Yating , Song Pengkang , Zhao Jiamin , Zhang Weipeng , Liu Xiangdong , Lv Xiaoyang , Zhao Junxing
Published: May 2024

This study aimed to explore the effects of neonatal vitamin A (VA) supplementation on testis development and spermatogenesis. A total of 32 newborn lambs were intramuscularly injected with corn oil (control group) or corn oil + 2500 IU/kg BW VA (VA group). They were slaughtered and sampled at 3 weeks and 8 months of age to analyze spermatogenesis, cell proliferation, hormone secretion, antioxidant status of the testis, and adult sheep sperm parameters. Compared with the control group, the expression of spermatogonial differentiation-related genes in VA group was up-regulated (P < 0.05). Testis weight, seminiferous tubule diameter, number of spermatogonium and spermatocyte, and sperm density increased significantly in VA group at 8 months of age (P < 0.05). Neonatal VA injection upregulated the expression of the cell proliferation marker PCNA and cell cycle-related genes in the testis (P < 0.05). VA increased the concentrations of testosterone (T), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) in the serum and upregulated steroidogenesis-related genes in the testis (P < 0.05). The antioxidant levels in the VA group were maintained at high levels. The total antioxidant capacity (T-AOC), antioxidant enzyme content and antioxidant-related genes were increased in the testis (P < 0.05). Furthermore, neonatal VA injection activated retinoic acid (RA) signaling to maintain the blood-testosterone barrier (BTB) in the testis of 3-week-old sheep. AMP-activated protein kinase (AMPK) and protein kinase B (AKT) signaling were also modulated in the sheep testis (P < 0.05). Taken together, VA supplementation in newborn rams promotes testis development and spermatogenesis to improve fertility.
Genetic diversity and phylogeographic dynamics of avihepadnavirus: a comprehensive full-length genomic view
Authors: Sikandar Muhammad , Shah Pir Tariq , Xing Li
Published: May 2024

Avihepadnavirus is a genus of the Hepadnaviridae family. It primarily infects birds, including species of duck, geese, cranes, storks, and herons etc. To understand the genetic relatedness and evolutionary diversity among avihepadnavirus strains, a comprehensive analysis of the available 136 full-length viral genomes (n = 136) was conducted. The genomes were classified into two major genotypes, i.e., GI and GII. GI viruses were further classified into 8 sub-genotypes including DHBV-I (duck hepatitis B virus-I), DHBV-II (Snow goose Hepatitis B, SGHBV), DHBV-III, RGHBV (rossgoose hepatitis B virus), CHBV (crane hepatitis B virus), THBV (Tinamou hepatitis B virus), STHBV (stork hepatitis B virus), and HHBV (Heron hepatitis B virus). DHBV-I contains two sub-clades DHBV-Ia and DHBV-Ib. Parrot hepatitis B virus (PHBV) stains fall into GII which appeared as a separate phylogenetic branch/clade. All the subtypes of viruses in GI and GII seem to be genetically connected with viruses of DHBV-I by multiple mutational steps in phylogeographic analysis. Furthermore, 16 potential recombination events among different sub-genotypes in GI and one in GII were identified, but none of which is inter-genotypic between GI and GII. Overall, the results provide a whole picture of the genetic relatedness of avihepadnavirus strains, which may assist in the surveillance of virus spreading.
Organizational Practices’ Role in Managing Open Innovation and Business Performance
Authors: Rabie Nada , Moustafa Ayman , Ghaithi Fatima Al
Published: May 2024

Given the ever-changing world of technological advances, and due to the fact that business entities strive for efficiency and cost reduction, open innovation (OI) has become the focus of academic and scholarly discussions. Furthermore, to increase their competitiveness, small and medium enterprises (SMEs) have started implementing OI practices. This study aims to investigate the impact of SMEs’ internal organizational practices on OI and the impact of the latter on SMEs’ business performance. This quantitative study, which was based on gathering insights from SMEs, sought to answer two research questions related to the effects of organizational practices on the adoption and management of OI processes in SMEs and the role of OI in accelerating the business performance of SMEs. The findings revealed that not all SMEs’ internal organizational practices have a positive impact on both OI and SMEs’ business performance. This study is among the earliest studies in the UAE and GCC region to explore the impact of specific internal organizational practices on SME OI adoption and its business performance. The present study contributes theoretically and practically to OI literature and assists SME managers in evaluating their internal organizational practices’ suitability for OI adoption.
E-Commerce Participation, Subjective Norms and Grassland Utilization Pressure: An Empirical Evidence of Herdsmen in Inner Mongolia, China
Authors: Tian Mingjun , Wu Yunhua
Published: May 2024

The general requirements of China’s rural revitalization strategy are industrial prosperity, ecological livability and rich life. However, the traditional livestock breeding model has struggled to balance the dual requirements of production development and ecological protection, and it is urgent to inject new impetus and explore new development paths. At present, e-commerce has become a bridge between pastoral areas and cities, herdsmen and consumers. E-commerce participation is not only an important starting point for herdsmen to increase the added value and profit space of livestock products, but also an effective way to change the original breeding behavior based on the premise of destroying grassland. Therefore, this paper presents an in-depth study on the issue of e-commerce enabling grassland ecological restoration, aiming to provide more scientific and effective guidance for e-commerce to be used to achieve a win–win situation in economy and ecology. Therefore, based on the data of 271 herdsmen in pastoral areas of Inner Mongolia, we used the OLS model and the two-stage least squares (2SLS) method to identify the direct impact of herdsmen’s e-commerce participation on grassland utilization pressure. The empirical results show that e-commerce participation can significantly decrease the grassland utilization pressure. The conclusion was still valid after alleviating endogeneity and conducting a robustness test. The results of a mechanism analysis suggest that the reduction effect of e-commerce participation on grassland utilization pressure is mainly due to price incentive, reputation incentive and place identity. Subjective norms can strengthen the inhibitory effect of e-commerce participation on grassland utilization pressure. Furthermore, a heterogeneity analysis demonstrates that e-commerce participation has a better, decreased impact on the grassland utilization pressure on the banners of China’s rural e-commerce demonstration county program. Under a counterfactual assumption, if herdsmen who can participate in e-commerce choose not to do this, their grassland utilization pressure will increase.
Design and Parameter Optimization of a Dual-Disc Trenching Device for Ecological Tea Plantations
Authors: Chen Weixiang , Ren Jinbo , Huang Weiliang , Chen Longbin , Weng Wuxiong , Chen Chongcheng , Zheng Shuhe
Published: May 2024

This paper addresses challenges in the application of existing colters in Chinese ecological tea plantations due to abundant straw roots and insufficient tillage depth. Aligned with the agronomic requirements of hilly eco-tea plantations, our study optimizes the structural advantages of the joint use of rotary tillage blades and double-disc colters to design an efficient trenching device. Our investigation explores the motion characteristics of a double-disc colter during deep trenching operations, in conjunction with rotary tillage blades. Employing discrete element method (DEM) simulations, this paper aims to minimize the working resistance and enhance the tillage depth stability. Single-factor experiments are conducted to determine the impact of key structural parameters on the tillage depth stability and working resistance. The optimal parameters are determined as a relative height of 80 mm to 120 mm, a 280 mm to 320 mm diameter for the double-disc colter, and a 10° to 14° angle between the two discs. The central composite design method is used to optimize the structural parameters of the double-disc colter. The results indicate that when the relative height is 82 mm, the diameter of the double-disc colter is 297 mm, and the angle between the two discs is 14°, the tillage depth stability performance reaches 91.64%. With a working resistance of merely 93.93 N, the trenching device achieves optimal operational performance under these conditions. Field validation testing shows a tillage depth stability coefficient of 92.37% and a working resistance of 104.2 N. These values deviate by 0.73% and 10.93%, respectively, from the simulation results, confirming the reliability of the simulation model. A field validation test further confirms that the operational performance of the colter aligns with the agronomic requirements of ecological tea plantations, offering valuable insights for research on trenching devices in such environments.
Deep Learning Pricing of Processing Firms in Agricultural Markets
Authors: Khalili Hamed
Published: May 2024

The pricing behavior of agricultural processing firms in input markets has large impacts on farmers’ and processors’ prosperity as well as the overall market structure. Despite analytical approaches to food processors’ pricing in agricultural input markets, the need for models to represent complex market features is urgent. Agent-based models (ABMs) serve as computational laboratories to understand complex markets emerging from autonomously interacting agents. Yet, individual agents within ABMs must be equipped with intelligent learning algorithms. In this paper, we propose supervised and unsupervised learning agents to simulate the pricing behavior of firms in agricultural markets’ ABMs. Supervised learning firms are pre-trained to accurately best respond to their competitors and are deemed to result in the market Nash equilibria. Unsupervised learning firms play a course of pricing interaction with their competitors without any pre-knowledge but based on deep reinforcement learning. The simulation results show that unsupervised deep learning firms are capable of approximating the pricing equilibria obtained by the supervised firms in different spatial market settings. Optimal discriminatory and uniform delivery pricing emerges in agricultural input markets with the high and intermediary importance placed on space. Free on board pricing emerges in agricultural input markets with small importance placed on space.
Transcriptome Analysis and Validation of Anthracnose Resistance Genes in Walnut Varieties
Authors: Li Xiuzhen , Wang Yuman , Zhao Long , Ding Wenxuan , Chen Sudan , Li Xueqiang , Li Peijie
Published: May 2024

Anthracnose is extremely detrimental to walnut production, because it seriously affects its yield. Therefore, this study aimed to identify anthracnose resistance genes in walnuts, to lay the foundation for cultivating novel anthracnose-resistant walnut varieties. In this study, the fruits of walnut varieties “Qinyou 1” and “Qinyou 2”, exhibiting relatively weak resistance to anthracnose, were used for transcriptome sequencing and identifying differentially expressed genes (DEGs). Thereafter, we used a quantitative real-time polymerase chain reaction to verify the reliability of the transcriptome data using the walnut varieties Qinyou 1, Qinyou 2, Qinyou 3, Liaohe 1, and Diamond 1. We identified 6326 DEGs post-infection in Qinyou 1, of which 3065 were upregulated and 3261 were downregulated. We also found 2055 DEGs (969 upregulated and 1086 downregulated genes) in the two varieties, after 6 days of anthracnose infection. Based on GO and KEGG enrichment analyses of the transcriptome data, we noted that the DEGs were primarily involved in metabolic processes, cell composition, cell structure, catalytic activity, and binding activity. Furthermore, KEGG functional enrichment analysis showed that the amino acid biosynthesis pathway and plant–pathogen interaction may be activated under anthracnose infection. We also noted that the expression levels of anthracnose resistance genes in different walnut varieties were significantly different. Thus, the findings of this study provide important genetic resources and molecular markers for the cultivation of novel anthracnose-resistant walnut varieties.
Fertilising Maize with Bio-Based Mineral Fertilisers Gives Similar Growth to Conventional Fertilisers and Does Not Alter Soil Microbiome
Authors: Barquero Marcia , Cazador Cinta , Ortiz-Liébana Noemí , Zotti Maurizio , Brañas Javier , González-Andrés Fernando
Published: May 2024

The production of mineral fertilisers relies heavily on mineral deposits that are becoming depleted or is based on processes that are highly energy demanding. In this context, and in line with the circular economy and the European Green Deal, the recovery of nitrogen (N), phosphorus (P), and potassium (K) from organic wastes using chemical technologies is an important strategy to produce secondary raw materials for incorporation into mineral fertilisers, partially replacing the traditional sources of N, P, and K. However, there are very few studies on the agronomic and environmental effects of such substitution. The aim of this work was to evaluate plant growth under microcosm conditions and the effect on the soil microbiome of mineral fertilisers in which part of the N, P, or K content comes from bio-based materials (BBMFs), namely ash, struvite, and a patented chemical process. The crop was maize, and a metataxonomic approach was used to assess the effect on the soil microbiome. The BBMF treatments were compared with a control treated with a conventional mineral fertiliser. The conventional fertiliser performed significantly better than the bio-based fertilisers in terms of maize biomass production at the first sampling point 60 days after sowing (DAS), but at the last sampling point, 90 DAS, the BBMFs showed comparable or even better biomass production than the conventional one. This suggests that BBMFs may have a slightly slower nutrient release rate. The use of fertiliser, whether conventional or BBMF, resulted in a significant increase in microbiome biodiversity (Shannon index), while it did not affect species richness. Interestingly, the use of fertilisers modulated the composition of the bacterial community, increasing the abundance of beneficial bacterial taxa considered to be plant-growth-promoting bacteria, without significant differences between the conventional mineral fertilisers and the BBMFs. The predominance of PGPRs in the rhizosphere of crops when BBMFs are used could be part of the reason why BBMFs perform similarly or even better than conventional fertilisers, even if the rate of nutrient release is slower. This hypothesis will be tested in future field trials. Thus, BBMFs are an interesting option to make the food chain more sustainable.
Analysis of Microbial Diversity in Rhizosphere Soil of Panax notoginseng under Different Water and Microbial Fertilizer Conditions
Authors: Yao Leilei , Kong Lei , Yang Qiliang , Nian Hongjuan , Liang Jiaping
Published: May 2024

Panax notoginseng is a highly regarded medicinal plant that has obstacles associated with continuous cropping. Understanding soil microorganisms is crucial, as they play a major role in this regard. However, soil microorganisms are affected by multiple factors; therefore, we need to conduct more in-depth research. This study investigated the combined effects of irrigation and microbial fertilizer treatments (J1F1, J1F2, J2F1, J2F2, J3F1, J3F2, and CK) on the diversity of bacterial and fungal microbial communities in the rhizosphere of Panax notoginseng. The bacterial 16S rRNA genes and fungal internal transcribed spacer (ITS) sequences were sequenced using Illumina HiSeq. The results showed that, without microbial fertilizer (CK), the microbial community abundance and diversity were significantly lower than in the other treatments; moreover, among the microbial fertilizer treatments, the microbial abundance in F1 was higher than that in F2. Under the same microbial fertilizer application, the incidence rate of Panax notoginseng root rot was J2 > J1 > J3, and the yield of Panax notoginseng was J3 > J2 > J1. Under the same irrigation conditions, the incidence rate of Panax notoginseng root rot was F1 > F2, and the yield of Panax notoginseng was F2 > F1. This study provides important guidance for Panax notoginseng plant microbiota and sustainable agriculture.
The Impact of Microplastic Concentration and Particle Size on the Germination and Seedling Growth of Pisum sativum L.
Authors: Li Yibo , Yang Genshen , Yu Chen , Lei Xiaoting , Xing Xuguang , Ma Xiaoyi , Sun Yan
Published: May 2024

Hydroponic experiments were conducted to investigate the effects of varying particle sizes (5 μm, 0.1 μm, and 0.08 μm) and concentrations (0, 50, 100, 200, 500, 1000, and 2000 mg/L) of polystyrene plastic microspheres (PS-MPs) on the germination and growth of P. sativum seeds in order to investigate the effects of the microplastic environment on crop seed germination and seedling growth. The findings demonstrated that PS-MPs significantly harmed P. sativum seed germination. Specifically, low concentrations or high particle sizes weakened or promoted P. sativum seed germination, indicating varying susceptibilities to PS-MP treatment at different particle sizes. The strongest inhibitory effect on growth was observed under small particle size (0.08 μm) and high concentration (2000 mg/L). Stress had less of an impact on P. sativum in environments with low concentrations of PS-MPs, but in environments with medium and high concentrations, physiological and biochemical indicators like germination rate, stem length, and root length were significantly impacted. Furthermore, the particle size and concentration of PS-MPs had an impact on the growth effect of P. sativum seedlings. The indices of P. sativum seed germination were significantly impacted by the particle size of PS-MPs, despite the fact that PS-MP concentration was low. When the concentration of PS-MPs is low, the effects of varying PS-MP particle sizes on seed germination and root length are as follows: 0.1 μm > 5 μm > 0.08 μm. The concentration of PS-MPs had a significant impact on how it affected the buildup of dry matter and photosynthetic pigment.
Seedling-YOLO: High-Efficiency Target Detection Algorithm for Field Broccoli Seedling Transplanting Quality Based on YOLOv7-Tiny
Authors: Zhang Tengfei , Zhou Jinhao , Liu Wei , Yue Rencai , Yao Mengjiao , Shi Jiawei , Hu Jianping
Published: May 2024

The rapid and accurate detection of broccoli seedling planting quality is crucial for the implementation of robotic intelligent field management. However, existing algorithms often face issues of false detections and missed detections when identifying the categories of broccoli planting quality. For instance, the similarity between the features of broccoli root balls and soil, along with the potential for being obscured by leaves, leads to false detections of “exposed seedlings”. Additionally, features left by the end effector resemble the background, making the detection of the “missed hills” category challenging. Moreover, existing algorithms require substantial computational resources and memory. To address these challenges, we developed Seedling-YOLO, a deep-learning model dedicated to the visual detection of broccoli planting quality. Initially, we designed a new module, the Efficient Layer Aggregation Networks-Pconv (ELAN_P), utilizing partial convolution (Pconv). This module serves as the backbone feature extraction network, effectively reducing redundant calculations. Furthermore, the model incorporates the Content-aware ReAssembly of Features (CARAFE) and Coordinate Attention (CA), enhancing its focus on the long-range spatial information of challenging-to-detect samples. Experimental results demonstrate that our Seedling-YOLO model outperforms YOLOv4-tiny, YOLOv5s, YOLOv7-tiny, and YOLOv7 in terms of speed and precision, particularly in detecting ‘exposed seedlings’ and ‘missed hills’-key categories impacting yield, with Average Precision (AP) values of 94.2% and 92.2%, respectively. The model achieved a mean Average Precision of 0.5 (mAP@0.5) of 94.3% and a frame rate of 29.7 frames per second (FPS). In field tests conducted with double-row vegetable ridges at a plant spacing of 0.4 m and robot speed of 0.6 m/s, Seedling-YOLO exhibited optimal efficiency and precision. It achieved an actual detection precision of 93% and a detection efficiency of 180 plants/min, meeting the requirements for real-time and precise detection. This model can be deployed on seedling replenishment robots, providing a visual solution for robots, thereby enhancing vegetable yield.
Research on a Biofilter for a Typical Application Scenario in China: Treatment of Pesticide Residue Wastewater in Orchards
Authors: Zeng Jin , Yuan Quanchun , Xu Wenzhi , Li Hailong , Li Menghui , Lei Xiaohui , Wang Wei , Lin Qiang , Li Xue , Xu Rui , Lyu Xiaolan
Published: May 2024

To reduce pesticide pollution and promote sustainable agricultural development in China, we designed a pilot-scale biofilter system to treat residual imidacloprid wastewater in an orchard. The biofilter system demonstrated a high rate of removal of imidacloprid from the biodegradation wastewater, with removal rates from the outlet exceeding 99% at different concentrations of pesticides. Among environmental factors, imidacloprid concentration at the inlet and biomixture significantly affected the activity of imidacloprid-degrading bacteria. The dominant microbial communities during the stable operation of the biofilter system included Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes at the phylum level and Bacillus, Methylobacter, and unclassified_f__Microbacteriaceae at the genus level. In future initiatives to improve biofilter performance and applicability, increasing attention should be paid to the dominant microbial communities, the number of biofilter units, and important environmental factors. Orchard workers in China should improve the existing treatment of residual pesticide wastewater to mitigate agricultural non-point source pollution.
Optimizing Maize Yield and Resource Efficiency Using Surface Drip Fertilization in Huang-Huai-Hai: Impact of Increased Planting Density and Reduced Nitrogen Application Rate
Authors: Wu Liqian , Zhang Guoqiang , Yan Zhenhua , Gao Shang , Xu Honggen , Zhou Jiaqiang , Li Dianjun , Liu Yi , Xie Ruizhi , Ming Bo , Xue Jun , Hou Peng , Li Shaokun , Wang Keru
Published: May 2024

Improving crop yield and resource utilization efficiency is essential for agricultural productivity. In the Huang-Huai-Hai maize region of China, optimizing planting density, nitrogen (N) application, and fertilization methods are key strategies for enhancing maize yield and N use efficiency. However, traditional approaches have often hindered these improvements. To address this issue, we conducted a study in Baoding, Hebei, from 2022 to 2023, focusing on planting density, the N application rate, and the fertilization method on grain yield, N use efficiency, water use efficiency (WUE), and economic benefits. The trial involved two planting densities: 6.0 × 104 plants ha−1 (D1, typical local density) and 9.0 × 104 plants ha−1 (D2). Five N application rates were tested: 0 (N0), 120 kg ha−1 (N1), 180 kg ha−1 (N2), 240 kg ha−1 (N3), and 300 kg ha−1 (N4). The control treatment (D1N4) utilized the local planting density and traditional fertilization methods. Our findings revealed a positive correlation between the maize yield and N application rate, with the maximum yields (13.78–13.88 t ha−1), high WUE (24.42–29.85 kg m−3), agronomic efficiency of N (AEN) (18.11–19.00 kg kg−1), and economic benefits (2.44 × 104–2.47 × 104 CNY ha−1) observed with D2N3 and surface drip fertilization. This was significantly higher than the yield and resource efficiency of traditional fertilization methods and saved fertilizer and production costs. Therefore, adopting surface drip fertilization, adjusting planting density, and optimizing N application rates proved effective in enhancing maize yield and resource utilization efficiency in the Huang-Huai-Hai maize region.
Identification of Molecular Profile of Ear Fibroblasts Derived from Spindle-Transferred Holstein Cattle with Ooplasts from Taiwan Yellow Cattle under Heat Stress
Authors: Lee Yu-Ju , Lee Jai-Wei , Huang Chao-Wei , Yang Kuo-Tai , Peng Shao-Yu , Yu Chi , Lee Yen-Hua , Lai I-Ling , Shen Perng-Chih
Published: May 2024

Global warming has a significant impact on the dairy farming industry, as heat stress causes reproductive endocrine imbalances and leads to substantial economic losses, particularly in tropical–subtropical regions. The Holstein breed, which is widely used for dairy production, is highly susceptible to heat stress, resulting in a dramatic reduction in milk production during hot seasons. However, previous studies have shown that cells of cows produced from reconstructed embryos containing cytoplasm (o) from Taiwan yellow cattle (Y) have improved thermotolerance despite their nuclei (n) being derived from heat-sensitive Holstein cattle (H). Using spindle transfer (ST) technology, we successfully produced ST-Yo-Hn cattle and proved that the thermotolerance of their ear fibroblasts is similar to that of Y and significantly better than that of H (p < 0.05). Despite these findings, the genes and molecules responsible for the different sensitivities of cells derived from ST-Yo-Hn and H cattle have not been extensively investigated. In the present study, ear fibroblasts from ST-Yo-Hn and H cattle were isolated, and differentially expressed protein and gene profiles were compared with or without heat stress (hs) (42 °C for 12 h). The results revealed that the relative protein expression levels of pro-apoptotic factors, including Caspase-3, -8, and -9, in the ear fibroblasts from the ST-Yo-Hn-hs group were significantly lower (p < 0.05) than those from the H-hs group. Conversely, the relative expression levels of anti-apoptotic factors, including GNA14 protein and the CRELD2 and PRKCQ genes, were significantly higher (p < 0.05) in the ear fibroblasts from the ST-Yo-Hn-hs group compared to those from the H-hs group. Analysis of oxidative phosphorylation-related factors revealed that the relative expression levels of the GPX1 gene and Complex-I, Complex-IV, CAT, and PGLS proteins were significantly higher (p < 0.05) in the ear fibroblasts from the ST-Yo-Hn-hs group compared to those from the H-hs group. Taken together, these findings suggest that ear fibroblasts from ST-Yo-Hn cattle have superior thermotolerance compared to those from H cattle due to their lower expression of pro-apoptotic factors and higher expression of oxidative phosphorylation and antioxidant factors. Moreover, this improved thermotolerance is attributed, at least partially, to the cytoplasm derived from more heat-tolerant Y cattle. Hence, using ST technology to produce more heat-tolerant H cattle containing Y cytoplasm could be a feasible approach to alleviate the negative impacts of heat stress on dairy cattle in tropical–subtropical regions.
A Qualitative Review of Patient Feedback for the OPAT (Outpatient Antimicrobial Therapy) Service in Bristol
Authors: Soni Shuchita , Harding Irasha , Jones Carys , Wade Sue , Norton Jenna , Pollock Jennifer Siobhan
Published: May 2024

Outpatient parenteral antimicrobial therapy (OPAT) aims to deliver intravenous antimicrobials to medically stable patients with complex infections outside of a hospital setting. There is good evidence to demonstrate the safety and efficacy of OPAT in the literature. Anecdotally, the feedback from patients has been positive, but only a few studies evaluate this topic in detail. The aim of this qualitative study was to examine patients’ experiences with and feedback on the OPAT service in Bristol, United Kingdom, which was established in 2021. A total of 92 patient feedback surveys were reviewed retrospectively, and thematic analysis was undertaken. Feedback from OPAT patients in our centre was overwhelmingly positive. The key themes identified were benefits to the patients, their friends, and family, and positive feedback about OPAT staff. The mean overall satisfaction score for OPAT was 9.6 out of 10. Areas to improve included communication between the OPAT and parent teams, improving OPAT capacity, and expansion of the service.
Pointwise Nonparametric Estimation of Odds Ratio Curves with R: Introducing the flexOR Package
Authors: Azevedo Marta , Meira-Machado Luís , Gude Francisco , Araújo Artur
Published: May 2024

The analysis of odds ratio curves is a valuable tool in understanding the relationship between continuous predictors and binary outcomes. Traditional parametric regression approaches often assume specific functional forms, limiting their flexibility and applicability to complex data. To address this limitation and introduce more flexibility, several smoothing methods may be applied, and approaches based on splines are the most frequently considered in this context. To better understand the effects that each continuous covariate has on the outcome, results can be expressed in terms of splines-based odds ratio (OR) curves, taking a specific covariate value as reference. In this paper, we introduce an R package, flexOR, which provides a comprehensive framework for pointwise nonparametric estimation of odds ratio curves for continuous predictors. The package can be used to estimate odds ratio curves without imposing rigid assumptions about their underlying functional form while considering a reference value for the continuous covariate. The package offers various options for automatically choosing the degrees of freedom in multivariable models. It also includes visualization functions to aid in the interpretation and presentation of the estimated odds ratio curves. flexOR offers a user-friendly interface, making it accessible to researchers and practitioners without extensive statistical backgrounds.
Reflection and Refraction: Multivalent Social Realism in the Work of Joaquín Sorolla
Authors: Vorsanger Rachel
Published: May 2024

Joaquin Sorolla’s Social Realist work Sad Inheritance! provides the grounds for this cross-sectional case study into Social Realism in Spain, Spanish politics at the turn of the twentieth century, and affect theory in art. By formally analyzing this work, presenting its differing receptions in France and Spain, and discussing the identity crisis that Spain experienced at the end of the twentieth century, all within the frame of Jill Bennett’s conception of practical aesthetics and affect in art, this article will show how Sorolla produced an image that had differing valences of affect depending on the context in which it was viewed. Through his singular pictorial strategies, Sorolla successfully created an image that was political and sentimental, controversial and appealing, fraught with emotion, and ultimately affective.
Drought Stress Might Induce Sexual Spatial Segregation in Dioecious Populus euphratica—Insights from Long-Term Water Use Efficiency and Growth Rates
Authors: Zhou Honghua , Ye Zhaoxia , Yang Yuhai , Zhu Chenggang
Published: May 2024

P. euphratica stands as the pioneering and dominant tree within desert riparian forests in arid and semi-arid regions. The aim of our work was to reveal why dioecious P. euphratica in natural desert riparian forests in the lower Tarim River exhibits sexual spatial distribution differences combined with field investigation, tree ring techniques, isotope analysis techniques, and statistical analyses. The results showed that P. euphratica was a male-biased population, with the operational sex ratio (OSR) exhibiting spatial distribution differences to variations in drought stress resulting from groundwater depth change. The highest OSR was observed under mild drought stress (groundwater depth of 6–7 m), and it was reduced under non-drought stress (groundwater depth below 6 m) or severe drought stress (groundwater depth exceeding 7 m). As drought stress escalated, the degradation and aging of the P. euphratica forest became more pronounced. Males exhibited significantly higher growth rates and WUEi than females under mild drought stress. However, under severe drought stress, males’ growth rates significantly slowed down, accompanied by significantly lower WUEi than in females. This divergence determined the sexual spatial segregation of P. euphratica in the natural desert riparian forests of the lower Tarim River. Furthermore, the current ecological water conveyance project (EWCP) in the lower Tarim River was hard to fundamentally reverse the degradation and aging of the P. euphratica forest due to inadequate population regeneration. Consequently, we advocated for an optimized ecological water conveyance mode to restore, conserve, and rejuvenate natural P. euphratica forests.
Changes of Solitude Behaviors among College Students: A Latent Transition Analysis
Authors: Liu Tour , Wan Fuyu , Lu Xurong
Published: May 2024

Solitude behaviors encompass four types: positive solitude, eccentricity, social avoidance, and loneliness. These four types of solitude behaviors are not entirely independent but can co-occur within individuals. Thus, the purpose of this study was to explore latent classes of solitude behaviors, their developmental patterns, and relevant influencing factors among college students. The Solitude Behavior Scale—Short Version was administered to a sample of college students. A total of 417 Chinese students completed a three-time longitudinal paper questionnaire. The data analysis was performed using Mplus 8.0 and SPSS 26.0. Harman’s single-factor test, latent class analysis (LCA), and latent transition analysis (LTA) were employed for subsequent analysis. The results revealed three classes: low solitude, moderate solitude, and high solitude, which exhibited temporal changes. Social avoidance and loneliness could facilitate transitions between high solitude and moderate solitude. Females and first-grade students exhibited higher transition probabilities than males and students not in the first grade. The incidence of moderate solitude in the not-first-grade group was significantly higher than that in the first-grade group. Finally, this study offers new insights into the dynamics of solitude behaviors and their association with gender and age.
Targeting Interactions between Fibroblasts and Macrophages to Treat Cardiac Fibrosis
Authors: Yang Bo , Qiao Yan , Yan Dong , Meng Qinghang
Published: May 2024

Excessive extracellular matrix (ECM) deposition is a defining feature of cardiac fibrosis. Most notably, it is characterized by a significant change in the concentration and volume fraction of collagen I, a disproportionate deposition of collagen subtypes, and a disturbed ECM network arrangement, which directly affect the systolic and diastolic functions of the heart. Immune cells that reside within or infiltrate the myocardium, including macrophages, play important roles in fibroblast activation and consequent ECM remodeling. Through both direct and indirect connections to fibroblasts, monocyte-derived macrophages and resident cardiac macrophages play complex, bidirectional, regulatory roles in cardiac fibrosis. In this review, we discuss emerging interactions between fibroblasts and macrophages in physiology and pathologic conditions, providing insights for future research aimed at targeting macrophages to combat cardiac fibrosis.
Cholesterol-Modified Anti-Il6 siRNA Reduces the Severity of Acute Lung Injury in Mice
Authors: Chernikov Ivan V. , Bachkova Irina K. , Sen’kova Aleksandra V. , Meschaninova Mariya I. , Savin Innokenty A. , Vlassov Valentin V. , Zenkova Marina A. , Chernolovskaya Elena L.
Published: May 2024

Small interfering RNA (siRNA) holds significant therapeutic potential by silencing target genes through RNA interference. Current clinical applications of siRNA have been primarily limited to liver diseases, while achievements in delivery methods are expanding their applications to various organs, including the lungs. Cholesterol-conjugated siRNA emerges as a promising delivery approach due to its low toxicity and high efficiency. This study focuses on developing a cholesterol-conjugated anti-Il6 siRNA and the evaluation of its potency for the potential treatment of inflammatory diseases using the example of acute lung injury (ALI). The biological activities of different Il6-targeted siRNAs containing chemical modifications were evaluated in J774 cells in vitro. The lead cholesterol-conjugated anti-Il6 siRNA after intranasal instillation demonstrated dose-dependent therapeutic effects in a mouse model of ALI induced by lipopolysaccharide (LPS). The treatment significantly reduced Il6 mRNA levels, inflammatory cell infiltration, and the severity of lung inflammation. IL6 silencing by cholesterol-conjugated siRNA proves to be a promising strategy for treating inflammatory diseases, with potential applications beyond the lungs.
Effects of Chronic Inflammatory Activation of Murine and Human Arterial Endothelial Cells at Normal Lipoprotein and Cholesterol Levels In Vivo and In Vitro
Authors: Mussbacher Marion , Basílio José , Belakova Barbora , Pirabe Anita , Ableitner Elisabeth , Campos-Medina Manuel , Schmid Johannes A.
Published: May 2024

The activation of endothelial cells is crucial for immune defense mechanisms but also plays a role in the development of atherosclerosis. We have previously shown that inflammatory stimulation of endothelial cells on top of elevated lipoprotein/cholesterol levels accelerates atherogenesis. The aim of the current study was to investigate how chronic endothelial inflammation changes the aortic transcriptome of mice at normal lipoprotein levels and to compare this to the inflammatory response of isolated endothelial cells in vitro. We applied a mouse model expressing constitutive active IκB kinase 2 (caIKK2)—the key activator of the inflammatory NF-κB pathway—specifically in arterial endothelial cells and analyzed transcriptomic changes in whole aortas, followed by pathway and network analyses. We found an upregulation of cell death and mitochondrial beta-oxidation pathways with a predicted increase in endothelial apoptosis and necrosis and a simultaneous reduction in protein synthesis genes. The highest upregulated gene was ACE2, the SARS-CoV-2 receptor, which is also an important regulator of blood pressure. Analysis of isolated human arterial and venous endothelial cells supported these findings and also revealed a reduction in DNA replication, as well as repair mechanisms, in line with the notion that chronic inflammation contributes to endothelial dysfunction.
Neuroretinal Cell Culture Model as a Tool for the Development of New Therapeutic Approaches for Oxidative Stress-Induced Ocular Diseases, with a Focus on Glaucoma
Authors: Fomo Kristian Nzogang , Perumal Natarajan , Manicam Caroline , Pfeiffer Norbert , Grus Franz H.
Published: May 2024

Glaucoma is a heterogeneous group of optic neuropathies characterized by a progressive degeneration of the retinal ganglion cells (RGCs), leading to irreversible vision loss. Nowadays, the traditional therapeutic approach to glaucoma consists of lowering the intraocular pressure (IOP), which does not address the neurodegenerative features of the disease. Besides animal models of glaucoma, there is a considerable need for in vitro experimental models to propose new therapeutic strategies for this ocular disease. In this study, we elucidated the pathological mechanisms leading to neuroretinal R28 cell death after exposure to glutamate and hydrogen peroxide (H2O2) in order to develop new therapeutic approaches for oxidative stress-induced retinal diseases, including glaucoma. We were able to show that glutamate and H2O2 can induce a decrease in R28 cell viability in a concentration-dependent manner. A cell viability of about 42% was found after exposure to 3 mM of glutamate and about 56% after exposure to 100 µM of H2O2 (n = 4). Label-free quantitative mass spectrometry analysis revealed differential alterations of 193 and 311 proteins in R28 cells exposed to 3 mM of glutamate and 100 µM of H2O2, respectively (FDR < 1%; p < 0.05). Bioinformatics analysis indicated that the protein changes were associated with the dysregulation of signaling pathways, which was similar to those observed in glaucoma. Thus, the proteomic alteration induced by glutamate was associated with the inhibition of the PI3K/AKT signaling pathway. On the other hand, H2O2-induced toxicity in R28 cells was linked to the activation of apoptosis signaling and the inhibition of the mTOR and ERK/MAPK signaling pathways. Furthermore, the data show a similarity in the inhibition of the EIF2 and AMPK signaling pathways and the activation of the sumoylation and WNT/β-catenin signaling pathways in both groups. Our findings suggest that the exposure of R28 cells to glutamate and H2O2 could induce glaucoma-like neurodegenerative features and potentially provide a suitable tool for the development of new therapeutic strategies for retinal diseases.
Public Perceptions of Rotator Cuff Tears
Authors: Devarasetty V. V. N. Manohar , Kuhn John E. , Bowman Eric N.
Published: May 2024

(1) Background: Full-thickness rotator cuff tears (RCTs) impact 25% of those over 60 and 50% over 80; however, minimal data exists on public understanding; (2) Methods: The primary outcome was to determine the public’s baseline understanding of RCTs utilizing a 36-question survey regarding anatomy and function, risk factors, diagnosis and treatment options, and expectations. Secondarily, we evaluated the effect of an educational video and informational handout created by the authors to improve understanding. Participants ≥ 18 years were recruited from the senior author’s clinic and online discussion platforms over a 5-month period; (3) Results: Baseline surveys were completed by 382 individuals: 56% men, 64% Caucasian, 27% with at least a master’s degree, and 56% with very little or no RCT knowledge. Mean correct answer scores improved from 47% to 68% posteducational intervention (p < 0.001). Males, higher education level, healthcare experience, and a higher self-rated understanding of RCTs were significantly correlated with higher survey performance (p < 0.001); (4) Conclusions: The public’s knowledge of RCTs at baseline was poor, with demographic factors correlating with survey performance. The educational intervention effectively enhanced participants’ understanding. By focusing on common misconceptions, this data can help clinicians align patient expectations and enhance patient outcomes.
The Correlation between Maternal and Neonatal Vit D (25(OH)D) Levels in Greece: A Cross-Sectional Study
Authors: Kokkinari Artemisia , Dagla Maria , Antoniou Evangelia , Lykeridou Aikaterini , Kyrkou Giannoula , Bagianos Kostas , Iatrakis Georgios
Published: May 2024

Background: Few studies have correlated maternal and neonatal Vit D (25(OH)D) levels at birth in Greece. We investigated this potential association, taking into account the administration or not of low doses (400–800 IU) of prenatal Vit D supplements. Our study contributes evidence not only to the small amount of existing literature regarding the above correlation, but also to the topic of maternal and neonatal vitamin D deficiency (VDD) during pregnancy in Mediterranean countries, such as Greece. Methods: A cross-sectional study was conducted on 248 neonates and their mothers from September 2019 to January 2022. Blood samples of 25(OH)D were studied at the time of delivery. Frequency counts and percentages were registered, and logistic regression was used to investigate the independent factors associated with maternal Vit D status. The Chi-square test and the Pearson coefficient were used to demonstrate a possible association between maternal and neonatal 25(OH)D levels. Results: Our findings show a high prevalence of VDD in Greek women and their newborns at birth. This was observed not only in women who did not receive Vit D supplements, but also in all the study groups, especially in the autumn and winter months. We observed that mothers who received low doses (400–800 IU) of prenatal Vit D supplements increased both their own 25(OH)D concentrations and those of their newborns; however, the latter did not seem to be completely covered by the prenatal administration of Vit D because, although their 25(OH)D concentrations increased, they never reached sufficient 25(OH)D levels, unlike their mothers who reached sufficient concentrations. Conclusions: Overall, this study highlights the strong association between maternal and neonatal 25(OH)D concentrations at the end of gestation. However, neonates tended to show even lower 25(OH)D concentrations relative to maternal 25(OH)D concentrations. The same phenomenon was observed irrespective of the administration of Vit D supplements during pregnancy. Moreover, this is what was observed concerning the administration of formulations with 400–800 IU of Vit D, which the doctors in our clinic used in the present study. In any case, more clinical studies related to the administration of higher doses of Vit D supplementation to pregnant women would lead to more reliable conclusions. Without a doubt, the measurement of maternal vitamin D status during pregnancy provides opportunities for preventive and therapeutic interventions in the mother–infant pair.