bluuebunny/crossref_metadata_2025 huggingface dataset:
Secure Inter-Frame Space Communications for Wireless LANs
Authors: Lee Il-Gu
Published: June 2018

The internet of things (IoTs) offers a wide range of consumer benefits, from personal portable devices to internet-connected infrastructure. The wireless local area network (WLAN) is one of the potential candidates for IoTs technology to connect billions of smart devices. Long-range WLAN is widely deployed in dense networks as an alternative to cellular networks or satellite internet access because of its low cost, high performance, and existing ecosystem. However, due to the nature of unregulated communications in industrial, scientific, and medical (ISM) bands, WLANs experience interferences from other radios such as radars and frequency hopping devices. Once interference is detected at a WLAN device, the channel is avoided and other channels become crowded. Thus, it degrades network throughput and channel utilization. In this paper, a secure inter-frame space communication system design is proposed for WLANs to share the ISM bands with other radio systems that generate periodic radio signals. The proposed secure inter-frame communication scheme achieves the goal by designing accurate radar detection and secure inter-frame space communication. The simulation results demonstrate that the proposed scheme significantly increases the receiver sensitivity and user datagram protocol throughput.
High-Throughput Incubation and Quantification of Agglutination Assays in a Microfluidic System
Authors: Castro David , Conchouso David , Kodzius Rimantas , Arevalo Arpys , Foulds Ian G.
Published: June 2018

In this paper, we present a two-phase microfluidic system capable of incubating and quantifying microbead-based agglutination assays. The microfluidic system is based on a simple fabrication solution, which requires only laboratory tubing filled with carrier oil, driven by negative pressure using a syringe pump. We provide a user-friendly interface, in which a pipette is used to insert single droplets of a 1.25-µL volume into a system that is continuously running and therefore works entirely on demand without the need for stopping, resetting or washing the system. These assays are incubated by highly efficient passive mixing with a sample-to-answer time of 2.5 min, a 5–10-fold improvement over traditional agglutination assays. We study system parameters such as channel length, incubation time and flow speed to select optimal assay conditions, using the streptavidin-biotin interaction as a model analyte quantified using optical image processing. We then investigate the effect of changing the concentration of both analyte and microbead concentrations, with a minimum detection limit of 100 ng/mL. The system can be both low- and high-throughput, depending on the rate at which assays are inserted. In our experiments, we were able to easily produce throughputs of 360 assays per hour by simple manual pipetting, which could be increased even further by automation and parallelization. Agglutination assays are a versatile tool, capable of detecting an ever-growing catalog of infectious diseases, proteins and metabolites. A system such as this one is a step towards being able to produce high-throughput microfluidic diagnostic solutions with widespread adoption. The development of analytical techniques in the microfluidic format, such as the one presented in this work, is an important step in being able to continuously monitor the performance and microfluidic outputs of organ-on-chip devices.
Matrix of Priorities for the Management of Visitation Impacts on the Geosites of Araripe UNESCO Global Geopark (NE Brazil)
Authors: Guimarães Eduardo , Sá Artur , Gabriel Ronaldo , Moreira Helena , Guimarães Jaqueliny , Bandeira Paulo , Silva João , Soares Rafael , Melo José
Published: June 2018

In order to safeguard the material and immaterial heritage and to foster delightful experiences for visitors, this investigation aimed to define a Matrix of Priorities for management of Visitation Impacts Management on the Geosites of Araripe UNESCO Global Geopark. With a quantitative approach involving the revision of documents, the methods used in this paper determined what the demands for visitation are as well as the impacts of these demands. We identified the offers for activities and occurrences in the geosites. Using this model, we calculated the following variables: (i) evident impacts, (ii) management profile, (iii) visitor demand and (iv) activity zone. We also classified the recommended management actions and presented the priority matrix. We classified the geosites of Ponte de Pedra, Riacho do Meio and Cachoeira de Missão Velha as requiring immediate or priority management action, in view of the high impacts of visitation evidenced. The geosites of Pedra Cariri, Floresta Petrificada and Parque dos Pterossauros were classified as programmed action, highlighting the difficulty of accessing them and the low demand for visitation. The geosites Pontal de Santa Cruz, Colina do Horto and Batateiras presented the best scores and require feedback action. It is emphasized that the deepening the investigations is required in order to produce and interpret the data complexity that guides the management of a dynamic territory such as a UNESCO Global Geopark.
Moho Depth and Crustal Architecture Beneath the Levant Basin from Global Gravity Field Model
Authors: Sampietro Daniele , Mansi Ahmed Hamdi , Capponi Martina
Published: June 2018

The study of the discontinuity between the Earth crust and upper mantle, the so-called Moho, and of the lithospheric architecture in general, has several important applications in exploration geophysics. For instance, it is used to facilitate the inversion of seismic-related data, in order to obtain important information on the sedimentary layers or to study the Earth’s heat flux. In this paper, the Levant crustal structure is being investigated starting from the inversion of gravity disturbances coming from a global geopotential field model based on ESA GOCE satellite mission integrated with seismic derived information. In the considered area, which is of particular interest because of its richness from the resources point of view, the deep crustal structure is still a matter of study due to the presence of a thick sequence of sedimentary layers, deposited within geological eras by the Nile River. Within the current work, the shape of the Oceanic domain in correspondence to the Herodotus Basin and the Cyprus Arc has been clearly defined. Moreover the nature of the Levantine Basin and of the Eratosthenes crust has been investigated by a set of ad hoc tests, finding the presence of continental crust. Finally, the Moho depth and the crustal density distribution have been retrieved. Several localized anomalies, in the Cyprus area, have been identified and modelled too, thus confirming the presence of heavy material, with a thickness up to 10 km, in the sedimentary layer and shallower part of the crust.
Global Warming Can Lead to Depletion of Oxygen by Disrupting Phytoplankton Photosynthesis: A Mathematical Modelling Approach
Authors: Sekerci Yadigar , Petrovskii Sergei
Published: June 2018

We consider the effect of global warming on the coupled plankton-oxygen dynamics in the ocean. The net oxygen production by phytoplankton is known to depend on the water temperature and hence can be disrupted by warming. We address this issue theoretically by considering a mathematical model of the plankton-oxygen system. The model is generic and can account for a variety of biological factors. We first show that sustainable oxygen production by phytoplankton is only possible if the net production rate is above a certain critical value. This result appears to be robust to the details of model parametrization. We then show that, once the effect of zooplankton is taken into account (which consume oxygen and feed on phytoplankton), the plankton-oxygen system can only be stable if the net oxygen production rate is within a certain intermediate range (i.e., not too low and not too high). Correspondingly, we conclude that a sufficiently large increase in the water temperature is likely to push the system out of the safe range, which may result in ocean anoxia and even a global oxygen depletion. We then generalize the model by taking into account the effect of environmental stochasticity and show that, paradoxically, the probability of oxygen depletion may decrease with an increase in the rate of global warming.
Preliminary Data Validation and Reconstruction of Temperature and Precipitation in Central Italy
Authors: Gentilucci Matteo , Barbieri Maurizio , Burt Peter , D’Aprile Fabrizio
Published: June 2018

This study provides a unique procedure for validating and reconstructing temperature and precipitation data. Although developed from data in Middle Italy, the validation method is intended to be universal, subject to appropriate calibration according to the climate zones analysed. This research is an attempt to create shared applicative procedures that are most of the time only theorized or included in some software without a clear definition of the methods. The purpose is to detect most types of errors according to the procedures for data validation prescribed by the World Meteorological Organization, defining practical operations for each of the five types of data controls: gross error checking, internal consistency check, tolerance test, temporal consistency, and spatial consistency. Temperature and precipitation data over the period 1931–2014 were investigated. The outcomes of this process have led to the removal of 375 records (0.02%) of temperature data from 40 weather stations and 1286 records (1.67%) of precipitation data from 118 weather stations, and 171 data points reconstructed. In conclusion, this work contributes to the development of standardized methodologies to validate climate data and provides an innovative procedure to reconstruct missing data in the absence of reliable reference time series.
Revisiting Japan’s Fictional Gardens: An Ecocritical Reading of Nature Imagery in Contemporary Architectural Essays
Authors: Vitorino Catarina
Published: June 2018

This paper inspects editorial production in the field of Japanese contemporary architecture, screening the contents of essays written during the last decade (2007–2010) by four selected authors in which a recurring interplay with nature-related subjects is noticeable. This analysis highlights the diversity and intrinsic individual originality of these books by relating their specific approach with the overall work themes of each architect and discussing the existence of a common ground among them. While investigating their philosophical and conceptual standpoints, the paper also attempts to contextualize these discourses both within the larger context of architectural theory—particularly of early postmodern ecological approaches—sustainable construction, and in the milieu of Japan, where an imprinted notion of harmonious coexistence between nature and culture has long been mystified from abroad and from within. By assessing its motives and influence and finally questioning the existence of a paradox amidst the multiple existing forms of paraenvironmental architecture, it is discussed whether these narratives and practices manage to communicate ecoliteracy with their audience. Architecture’s inherent traits as a visual, perceptive, and cognitive discipline to reflect contemporary environmental conflicts and encourage paradigm change are also highlighted.
Adverse Events Following Immunization in Brazil: Age of Child and Vaccine-Associated Risk Analysis Using Logistic Regression
Authors: Lopes Sílvia R.C. , Perin João L.R. , Prass Taiane S. , Carvalho Sandra Maria D. , Lessa Sérgio C. , Dórea José G.
Published: June 2018

Objective: Vaccines are effective in controlling and eradicating infectious diseases. However, adverse events following immunization (AEFI) can occur in susceptible individuals. The objective of this study was to analyze the Brazilian AEFI database and compare eight vaccines in order to profile risks of AEFIs related to the mandated pediatric schedule of immunization, considering the age and sex of the child, type of vaccine, and reported adverse events. Methods: We analyzed the Brazilian AEFI database integrating reports between 2005 and 2010 for children less than 10-years old immunized with eight mandated vaccines: diphtheria, pertussis, tetanus, Haemophilus influenzae type b (TETRA); diphtheria, tetanus, and pertussis (DTP); Bacillus Calmette–Guerin (BCG); oral poliovirus vaccine (OPV); measles, mumps, and rubella (MMR); oral rotavirus vaccine (ORV); hepatitis B (HB); and yellow fever (YF). We compared the children’s age regarding types of AEFI, evaluated AEFI factors associated with the chance of hospitalization of the child, and estimated the chance of notification of an AEFI as a function of the type of vaccine. In total, 47,105 AEFIs were observed for the mandated vaccines. Results: The highest AEFI rate was for the TETRA vaccine and the lowest was for the OPV vaccine, with 60.1 and 2.3 events per 100,000 inoculations, respectively. The TETRA vaccine showed the highest rate of hypotonic hyporesponsive episode, followed by convulsion and fever. The MMR and YF vaccines were associated with generalized rash. BCG was associated with enlarged lymph glands but showed the largest negative (protective) association with hyporesponsive events and seizures. Compared with children aged 5–9-years old, young children (<1 year) showed significantly higher odds of hospitalization. Conclusions: The Brazilian AEFI registry is useful to compare the magnitude and certain characteristics of adverse events associated with mandated pediatric vaccines.
Quantitative Microbial Risk Assessment and Opportunist Waterborne Infections–Are There Too Many Gaps to Fill?
Authors: Bentham Richard , Whiley Harriet
Published: June 2018

Quantitative microbial risk assessment (QMRA) is a relatively new approach in identifying health risks associated with the ubiquitous presence of pathogens and opportunists in the human environment. The methodology builds on experimental and meta-analytical data to identify measurable factors that contribute to, and can quantify, the likely extent of disease given a particular exposure. Early modelling was particularly focused on food-borne disease, and subsequently water-borne disease, with the emphasis focused on ingestion and its role in enteric disease. More recently, there has been a focus on translating these principles to opportunist waterborne infections (OWI) with primary focus on Legionella spp. Whereas dose and susceptibility are well documented via the ingestion route of exposure there is considerably less certainty regarding both factors when understanding Legionella spp. and other OWI. Many OWI can arise through numerous routes of transmission with greatly differing disease presentations. Routes of Legionella spp. infection do not include ingestion, but rather aspiration and inhalation of contaminated water are the routes of exposure. The susceptible population for OWI is a vulnerable sub-set of the population unlike those associated with enteric disease pathogens. These variabilities in dose, exposure and susceptibility call in to question whether QMRA can be a useful tool in managing risks associated with OWI. Consideration of Legionella spp. as a well-documented subject of research calls into question whether QMRA of OWI is likely to be a useful tool in developing risk management strategies.
Physical Activity, Lifestyle Factors and Oxidative Stress in Middle Age Healthy Subjects
Authors: Carraro Elisabetta , Schilirò Tiziana , Biorci Felicina , Romanazzi Valeria , Degan Raffaella , Buonocore Daniela , Verri Manuela , Dossena Maurizia , Bonetta Sara , Gilli Giorgio
Published: June 2018

Oxidative stress (OS) has been recognized to play a primary role in many acute and chronic diseases. Environmental and lifestyle factors, such as physical activity and dietary intake are involved in the oxidative balance, but their specific influence remains unclear. In order to contribute to a greater characterization of the oxidative status in relation to exercise training and to environmental and lifestyle factors, different biomarkers—pro-oxidant capacity (d-ROMs), anti-oxidant capacity (BAP), radical scavenging activity (DPPH) and DNA damage (8-OHdGuo)—were measured in biological samples of a group of healthy middle aged subjects. The evaluation of the investigated biomarkers highlighted a significant effect of exercise training on OS, measured as d-ROMs and 8OhdGuo, in subjects playing regular physical activity. An association of the OS status measured by DPPH and 8-OhdGuo with the condition of living in urban high traffic areas was also found. Otherwise dietary habits did not reveal any significant effect on OS levels by the investigated biomarkers. As a whole the results obtained in this investigation suggested that a correct lifestyle, with regular physical activity practice, contributes to control the OS status in middle age subjects.
An Overview of Coverage of BCG Vaccination and Its Determinants Based on Data from the Coverage Survey in Zhejiang Province
Authors: Hu Yu , Chen Yaping , Liang Hui , Wang Ying
Published: June 2018

To assess the Bacille Calmette-Guérin (BCG) vaccination coverage in Zhejiang province and to investigate predictors of the BCG vaccination, we used data from the 2017 Zhejiang provincial coverage survey. Demographic and immunization data on the selected children, their mothers, and their families were also collected by using a pre-tested questionnaire. BCG scars were verified among children who were available at the moment of survey. Coverage of BCG and other expanded program on immunization (EPI) vaccines scheduled before the first year of life was calculated. BCG coverage through the scar assessment and timeliness of BCG were also presented. Multivariate analyses of the predictors associated with the BCG vaccination and its timeliness were conducted separately. In total, 1393 children agreed to participate in the survey and presented the immunization cards. Of them, the coverage of BCG was 92.0% and 88.3% received the BCG within the first 28 days after birth. Besides this, 1282 out of the 1393 children were screened for the BCG scars and 97.1% of them had developed the scars. The multivariable logistic regression analyses indicated that hospital delivery, higher maternal education, a mother with no job, and a resident child were positively associated with the higher BCG vaccination coverage and its timely administrations. BCG coverage was optimal and it was administered in a timely manner. The majority of children vaccinated with BCG developed scars. Tailored interventions should be more greatly focused on and targeted to children with the risk factors identified in this study.
A Qualitative Study of the Development of Health Literacy Capacities of Participants Attending a Community-Based Cardiovascular Health Programme
Authors: McKenna Verna B. , Sixsmith Jane , Barry Margaret M.
Published: June 2018

Health literacy is a critical determinant of health, which can empower individuals and lead to engagement in collective health promotion action and is also a crucial component in the self-management of illness. The current study moves beyond a focus on functional health literacy and presents findings from a longitudinal qualitative (LQ) study consisting of three phases. This paper presents findings from the second phase of the study, which assessed the development of health literacy capacities of individuals attending a structured cardiovascular risk reduction programme in Ireland. The study objectives were to: explore perceptions of changes in interactions and information exchange within health consultations; identify the facilitators associated with changes in health literacy capacities; assess developments in engagement with broader contexts for health literacy capacities. A LQ study design was undertaken, which employed repeat interview methodology with 19 participants (aged 36–76 years) 12 weeks after beginning a structured cardiovascular risk reduction programme. Health literacy levels were assessed using the HLS-EU 47 item instrument in phase 1 (68% limited health literacy (HL), 32% adequate health literacy). A semi-structured interview guide, (informed by Sørensen’s conceptual model of health literacy), was used to explore the development of health literacy and to identify changes in knowledge, attitudes and experiences over time. Thematic analysis was used, informed by aspects of Saldaña’s framework for longitudinal qualitative data analysis. All participants reported having acquired increased understanding of issues relevant to their health and self-care. Participants described health literacy capacities that incorporate aspects of all levels of health literacy (functional, interactive and critical). Core themes were identified corresponding to changes in these levels: re-engagement with health information and increased understanding of risk and protective factors (changes in functional health literacy); changes in interactions with healthcare providers (HCP) (changes in interactive health literacy); enhanced psychological insights and understanding the broader determinants of health (changes in critical health literacy). Findings support the development of health literacy capacities across the functional, interactive and critical health literacy domains. Participants are capable of locating responsibility for health beyond the individual level and are making sense of knowledge within their own social contexts. Individuals, regardless of their initial health literacy levels, are capable of engaging with broader issues that can impact on their health and can be supported to develop these critical health literacy capacities.
Mental Health Related Stigma as a ‘Wicked Problem’: The Need to Address Stigma and Consider the Consequences
Authors: Henderson Claire , Gronholm Petra C.
Published: June 2018

Recent reviews on the evidence base for mental health related stigma reduction show that under certain conditions interpersonal contact is effective in promoting more positive attitudes, reduced desire for social distance, and increased stigma related knowledge (knowledge which disconfirms beliefs based on stereotypes). Short-term interventions may have effects that are attenuated over time; longer term programmes may support sustained improvements, but research following up long-term interventions is scarce. However, the effectiveness of these interventions should not obscure the nature of stigma as a social problem. In this article we describe stigma as a ‘wicked problem’ to highlight some implications for intervening against stigma and evaluating these efforts. These include the risks of unintended consequences and the need to continually reformulate the concept of stigma, to ensure that tackling stigma at the structural, interpersonal, and intrapersonal levels become part of the core business of stakeholder organisations. We compare the main targets of anti-stigma programmes with what is known about the sources of stigma and discrimination and their impacts to identify targets for future intervention. In some cases, interventions have been directed at the interpersonal level when structural level intervention is also needed; in others, systematic reviews have not so far identified any interventions.
Using the Healthy Community Assessment Tool: Applicability and Adaptation in the Midwest of Western Australia
Authors: Tsou Christina , Green Charmaine , Gray Gordon , Thompson Sandra Claire
Published: June 2018

Population-based studies have associated poor living conditions with the persistent disparity in the health of Aboriginal and non-Aboriginal Australians. This project assesses the applicability of the Health Community Assessment Tool and its role in improving the environment of a small community in the Midwest of Western Australia (WA). The action research cycles started with the initial reflection on the suitability of the HCAT version 2 for the local community context and whether it was fit-for-purpose. The researcher provided ‘critical companionship’, while the participants of the study were invited to be co-researchers (the Assessors) who critically examined the HCAT and assess the community. The relevant domains to the serviced town (an outer regional community) were pest control and animal management; healthy housing; food supply; community vibrancy, pride and safety; reducing environmental tobacco smoke; and promoting physical activity. The Assessors found the HCAT descriptors mostly aligned with their community context but found some of the items difficult to apply. Based on participant’s suggestions, some of the original scoring scales were reformatted. School attendance and illicit drug use were identified as a key outcome indicator for youth but were missing from the HCAT. The HCAT domains applied helped streamlining core business of agencies in the local community. The face validity of HCAT items were confirmed in this research with minor adjustments to reflect local context. Youth engagement to education is of high community concern and the development of an item would create similar interagency collaborative dialogues.
The Relationship between Air Pollution and Depression in China: Is Neighbourhood Social Capital Protective?
Authors: Wang Ruoyu , Xue Desheng , Liu Ye , Liu Penghua , Chen Hongsheng
Published: June 2018

There is increasing evidence from the developed world that air pollution is significantly related to residents’ depressive symptoms; however, the existence of such a relationship in developing countries such as China is still unclear. Furthermore, although neighbourhood social capital is beneficial for health, whether it is a protective factor in the relationship between health and environment pollution remains unclear. Consequently, we examined the effects of cities’ PM2.5 concentrations on residents’ depressive symptoms and the moderating effects of neighbourhood social capital, using data from the 2016 wave of China Labourforce Dynamics Survey and the real-time remote inquiry website of Airborne Fine Particulate Matter and Air Quality Index. Results showed that PM2.5 concentrations and neighbourhood social capital may increase and decrease respondents’ depressive symptoms, respectively. Notably, neighbourhood social capital decreased the negative effect of PM2.5 concentrations on respondents’ depressive symptoms. These analyses contributed to the understanding of the effect of air pollution on mental health in China and confirmed that neighbourhood social capital were protective factors in the relationship between health and environment hazards.
Vascular Hyperactivity in the Rat Renal Aorta Participates in the Association between Immune Complex-Mediated Glomerulonephritis and Systemic Hypertension
Authors: Pérez-Torres Israel , Moguel-González Bernardo , Soria-Castro Elizabeth , Guarner-Lans Verónica , Avila-Casado María del Carmen , Goes Teresa Imelda Fortoul Vander
Published: June 2018

Introduction: systemic hypertension (SH) involving endothelial dysfunction contributes to immune complex-mediated glomerulonephritis (ICGN). Objective, we demonstrate a relationship between ICGN and SH by analyzing vascular reactivity in renal aortic rings. Methods: 48 male Wistar rats were divided into four groups: (a) control (C); (b) injected with bovine serum albumin (BSA); (c) receiving 200 mg/L NAME (an analog of arginine that inhibits NO production) in drinking water; and (d) receiving BSA and 200 mg/L NAME. Rats were pre-immunized subcutaneously with BSA and Freund’s adjuvant. After 10 days, groups (b) and (c) received 1 mg/mL of BSA in saline intravenous (IV) daily for 35 days. The urine of 24 h was measured at days 0, 15, 30 and 45. Results: vascular reactivity to norepinephrine (NE), acetylcholine (Ach) and NAME were tested. Creatinine clearance, vasodilatation, eNOS and elastic fibers were diminished (p ≤ 0.001). Blood pressure, vasoconstriction, iNOS were increased, and glomerular alterations were observed in groups (b), (c) and (d) when compared to group (a) (p ≤ 0.001). Conclusions: SH contributes to the development of progressive renal disease in ICGN. Alterations of the vascular reactivity are mediated by the endothelium in the renal aorta. Thus, the endothelium plays a determinant role in the production of vasoactive substances such as NO during this process.
Exploring the Determinants of Perceived Risk of Middle East Respiratory Syndrome (MERS) in Korea
Authors: Kim Sunhee , Kim Seoyong
Published: June 2018

The world is turning into a risky society. Although modernization based on the developments in science and technology has increased individuals’ well-being and wealth, the perceived risk toward the complex technological system has increased. In a risky society, social accidents amplify the existing fear among individuals. It is generally assumed that each value, perception, and resource influences the fear of risk. However, very few studies have tested these three factors together within an integrated causal model. Therefore, the present study aimed to examine the determinants that influence the perceived risk in cases of Middle East Respiratory Syndrome (MERS), a deadly epidemic disease, in Korea. Based on the theoretical model, we analyzed the survey data collected from respondents (N = 814) in Korea. After controlling for variables such as sociodemographic characteristics, we examined how three competing factors, i.e., value, perception, and resource, influence the perceived risk of MERS. The analysis showed that trust and vulnerability variables in the perception factor, health state, and perceived knowledge in the resource factor had a significant impact on the perceived risk of MERS.
Acute Lymphoblastic Leukemia with Hypereosinophilia in a Child: Case Report and Literature Review
Authors: Ferruzzi Valentina , Santi Elisa , Gurdo Grazia , Arcioni Francesco , Caniglia Maurizio , Esposito Susanna
Published: June 2018

Background: Hypereosinophilia in children can be primary or secondary. Numerous malignant diseases can cause hypereosinophilia, but it is seldom caused by acute lymphoblastic leukemia (ALL). In the event of protracted hypereosinophilia, it is extremely important to make a correct differential diagnosis. Case presentation: We present the case of an 11-year-old boy of Moroccan origin with ALL with hypereosinophilic onset (eosinophils in peripheral blood, 10,000/µL) in the absence of other signs of neoplastic disease, and compare this case with 61 similar cases in the literature. Following hospital admission, the patient initially presented with headache-caused nocturnal awakenings, evening fever, and cough, and he also lost approximately 7 kg in weight in a month not associated with sweating or itching. We first performed bone marrow aspiration, which showed an increase in eosinophils without cellular morphological abnormalities, and bone marrow immunophenotyping showed that 4.5% of cells had a phenotype compatible with lymphoid blasts. A lumbar puncture was negative. Given the poor marrow involvement, it was necessary to repeat a new bone marrow aspiration two days later, which showed an increase in blasts to 14%. A concomitant bone marrow biopsy showed an infiltration of blasts typical of B-cell ALL equal to 20–30% with associated hypereosinophilia. Cytogenetic analysis showed an hyperdiploid karyotype: 53–55, XY, +X, add(1)(q21q25), +4, +9, +10, +14, +2, +1, +21/46, XY. Conclusions: ALL is one of the possible causes of persistent hypereosinophilia. In patients with ALL and hypereosinophilia, peripheral hypereosinophilia can precede the appearance of blasts. Due to the negative prognosis and the increased risk of complications in these patients, bone marrow aspiration and biopsy are recommended if common causes of secondary hypereosinophilia are excluded.
Evaluation of Arts based Courses within a UK Recovery College for People with Mental Health Challenges
Authors: Stevens Joanna , Butterfield Catherine , Whittington Adrian , Holttum Sue
Published: June 2018

No previous studies have evaluated arts based recovery college courses. Yet arts may assist in personal recovery, as often defined by service users, through social connection and personal meaning. This interdisciplinary study evaluated (i) whether self-reported wellbeing and arts activities increased following arts based recovery college courses, and (ii) how students, peer trainers and artist-trainers understood courses’ impact. The design was mixed-methods. Of 42 service user students enrolling, 39 completed a course and 37 consented to provide data. Of these, 14 completed pre and post course questionnaires on mental wellbeing and 28 on arts participation. Post course focus groups were held with six of eight peer trainers and five of seven artist-trainers, and 28 students gave written feedback. Twenty-four students were interviewed up to three times in the subsequent nine months. There were statistically significant increases in self-reported mental wellbeing and range of arts activities following course attendance. At follow-up 17 of 24 students reported improved mental wellbeing, while seven reported little or no change. Some spoke of increased social inclusion and continuing to use skills learned in the course to maintain wellbeing. Initial in-course experience of ‘artistic growth’ predicted follow-up reports of improvement. Future controlled studies should employ standardized measures of social inclusion and arts participation.
Assessment of Groundwater Nitrate Pollution Potential in Central Valley Aquifer Using Geodetector-Based Frequency Ratio (GFR) and Optimized-DRASTIC Methods
Authors: Shrestha Anil , Luo Wei
Published: June 2018

Groundwater nitrate contamination in the Central Valley (CV) aquifer of California is widespread throughout the valley because of excess nitrogen fertilizer leaching down into the aquifer. The percolation of nitrate depends on several hydrogeological conditions of the valley. Groundwater contamination vulnerability mapping uses hydrogeologic conditions to predict vulnerable areas. This paper presents a new Geodetector-based Frequency Ratio (GFR) method and an optimized-DRASTIC method to generate nitrate vulnerability index values for the CV. The optimized-DRASTIC method combined the individual weights and rating values for Depth to water, Recharge rate, Aquifer media, Soil media, Topography, Impact of vadose zone, and Hydraulic conductivity. The GFR method incorporated the Frequency-Ratio (FR) method to derive rating values and the Geodetector method to derive relative Power of Determinant (PD) values as weights to generate nitrate susceptibility index map. The optimized-DRASTIC method generated very-high to high index values in the eastern part of the CV. The GFR method showed very-high index values in most part of the San Joaquin and Tulare basin. The quantitatively derived rating values and weights in the GFR method improved the vulnerability index and showed better consistency with the observed nitrate contamination pattern than optimized-DRASTIC index, suggesting that GFR is a better method for groundwater contamination vulnerability mapping in the CV aquifer.
Downregulation of α-Melanocyte-Stimulating Hormone-Induced Activation of the Pax3-MITF-Tyrosinase Axis by Sorghum Ethanolic Extract in B16F10 Melanoma Cells
Authors: Lee Da Hyun , Ahn Sung Shin , Kim Jung-Bong , Lim Yoongho , Lee Young Han , Shin Soon Young
Published: June 2018

Ultraviolet irradiation-induced hyperpigmentation of the skin is associated with excessive melanin production in melanocytes. Tyrosinase (TYR) is a key enzyme catalyzing the rate-limiting step in melanogenesis. TYR expression is controlled by microphthalmia-associated transcription factor (MITF) expression. Sorghum is a cereal crop widely used in a variety of foods worldwide. Sorghum contains many bioactive compounds and is beneficial to human health. However, the effects of sorghum in anti-melanogenesis have not been well characterized. In this study, the biological activity of sorghum ethanolic extract (SEE) on α-melanocyte-stimulating hormone (α-MSH)-induced TYR expression was evaluated in B16F10 melanoma cells. SEE attenuated α-MSH-induced TYR gene promoter activity through the downregulation of the transcription factor MITF. We found that paired box gene 3 (Pax3) contributes to the maximal induction of MITF gene promoter activity. Further analysis demonstrated that SEE inhibited α-MSH-induced Pax3 expression. The collective results indicate that SEE attenuates α-MSH-induced TYR expression through the suppression of Pax3-mediated MITF gene promoter activity. Targeting the Pax3-MITF axis pathway could be considered a potential strategy to increase the efficacy of anti-melanogenesis.
Biomimetic Layer-by-Layer Self-Assembly of Nanofilms, Nanocoatings, and 3D Scaffolds for Tissue Engineering
Authors: Zhang Shichao , Xing Malcolm , Li Bingyun
Published: June 2018

Achieving surface design and control of biomaterial scaffolds with nanometer- or micrometer-scaled functional films is critical to mimic the unique features of native extracellular matrices, which has significant technological implications for tissue engineering including cell-seeded scaffolds, microbioreactors, cell assembly, tissue regeneration, etc. Compared with other techniques available for surface design, layer-by-layer (LbL) self-assembly technology has attracted extensive attention because of its integrated features of simplicity, versatility, and nanoscale control. Here we present a brief overview of current state-of-the-art research related to the LbL self-assembly technique and its assembled biomaterials as scaffolds for tissue engineering. An overview of the LbL self-assembly technique, with a focus on issues associated with distinct routes and driving forces of self-assembly, is described briefly. Then, we highlight the controllable fabrication, properties, and applications of LbL self-assembly biomaterials in the forms of multilayer nanofilms, scaffold nanocoatings, and three-dimensional scaffolds to systematically demonstrate advances in LbL self-assembly in the field of tissue engineering. LbL self-assembly not only provides advances for molecular deposition but also opens avenues for the design and development of innovative biomaterials for tissue engineering.
Butterbur Leaves Attenuate Memory Impairment and Neuronal Cell Damage in Amyloid Beta-Induced Alzheimer’s Disease Models
Authors: Kim Namkwon , Choi Jin Gyu , Park Sangsu , Lee Jong Kil , Oh Myung Sook
Published: June 2018

Alzheimer’s disease (AD) is the most prevalent neurodegenerative disease, and is characterized by the accumulation of amyloid beta (Aβ) as a pathological hallmark. Aβ plays a central role in neuronal degeneration and synaptic dysfunction through the generation of excessive oxidative stress. In the present study, we explored whether leaves of Petasites japonicus (Siebold & Zucc.) Maxim. (PL), called butterbur and traditionally used in folk medicine, show neuroprotective action against Aβ25–35 plaque neurotoxicity in vitro and in vivo. We found that PL protected Aβ25–35 plaque-induced neuronal cell death and intracellular reactive oxygen species generation in HT22 cells by elevating expression levels of phosphorylated cyclic AMP response element-binding protein, heme oxygenase-1, and NAD(P)H quinine dehydrogenase 1. These neuroprotective effects of PL were also observed in Aβ25–35 plaque-injected AD mouse models. Moreover, administration of PL diminished Aβ25–35 plaque-induced synaptic dysfunction and memory impairment in mice. These findings lead us to suggest that PL can protect neurons against Aβ25–35 plaque-induced neurotoxicity and thus may be a potential candidate to regulate the progression of AD.
Connexins and Pannexins: Important Players in Tumorigenesis, Metastasis and Potential Therapeutics
Authors: Graham Sheila V. , Jiang Jean X. , Mesnil Marc
Published: June 2018

Since their characterization more than five decades ago, gap junctions and their structural proteins—the connexins—have been associated with cancer cell growth. During that period, the accumulation of data and molecular knowledge about this association revealed an apparent contradictory relationship between them and cancer. It appeared that if gap junctions or connexins can down regulate cancer cell growth they can be also implied in the migration, invasion and metastatic dissemination of cancer cells. Interestingly, in all these situations, connexins seem to be involved through various mechanisms in which they can act either as gap-junctional intercellular communication mediators, modulators of signalling pathways through their interactome, or as hemichannels, which mediate autocrine/paracrine communication. This complex involvement of connexins in cancer progression is even more complicated by the fact that their hemichannel function may overlap with other gap junction-related proteins, the pannexins. Despite this complexity, the possible involvements of connexins and pannexins in cancer progression and the elucidation of the mechanisms they control may lead to use them as new targets to control cancer progression. In this review, the involvements of connexins and pannexins in these different topics (cancer cell growth, invasion/metastasis process, possible cancer therapeutic targets) are discussed.
LAT1-Targeting Thermoresponsive Fluorescent Polymer Probes for Cancer Cell Imaging
Authors: Matsuura Minami , Ohshima Mariko , Hiruta Yuki , Nishimura Tomohiro , Nagase Kenichi , Kanazawa Hideko
Published: June 2018

L-type amino acid transporter 1 (LAT1) is more highly expressed in cancer cells compared with normal cells. LAT1 targeting probes would therefore be a promising tool for cancer cell imaging. In this study, LAT1-targeting thermoresponsive fluorescent polymer probes based on poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide) (P(NIPAAm-co-DMAAm)) were synthesized and their affinity for LAT1 was evaluated. The synthesized polymer probes interacted with LAT1 on HeLa cells, and inhibition of l-[3H]-leucine, one of the substrates for LAT1 uptake, was investigated. l-Tyrosine-conjugated P(NIPAAm-co-DMAAm) inhibited the uptake of l-[3H]-leucine, while P(NIPAAm-co-DMAAm) and l-phenylalanine-conjugated P(NIPAAm-co-DMAAm) did not. This result indicated that l-tyrosine-conjugated polymer has a high affinity for LAT1. The fluorescent polymer probes were prepared by modification of a terminal polymer group with fluorescein-5-maleimide (FL). Above the polymer transition temperature, cellular uptake of the polymer probes was observed because the polymers became hydrophobic, which enhanced the interaction with the cell membrane. Furthermore, quantitative analysis of the fluorescent probe using flow cytometry indicated that l-tyrosine-conjugated P(NIPAAm-co-DMAAm)-FL shows higher fluorescence intensity earlier than P(NIPAAm-co-DMAAm)-FL. The result suggested that cellular uptake was promoted by the LAT1 affinity site. The developed LAT1-targeting thermoresponsive fluorescent polymer probes are expected to be useful for cancer cell imaging.
Current Knowledge on Endocrine Disrupting Chemicals (EDCs) from Animal Biology to Humans, from Pregnancy to Adulthood: Highlights from a National Italian Meeting
Authors: Street Maria Elisabeth , Angelini Sabrina , Bernasconi Sergio , Burgio Ernesto , Cassio Alessandra , Catellani Cecilia , Cirillo Francesca , Deodati Annalisa , Fabbrizi Enrica , Fanos Vassilios , Gargano Giancarlo , Grossi Enzo , Iughetti Lorenzo , Lazzeroni Pietro , Mantovani Alberto , Migliore Lucia , Palanza Paola , Panzica Giancarlo , Papini Anna Maria , Parmigiani Stefano , Predieri Barbara , Sartori Chiara , Tridenti Gabriele , Amarri Sergio
Published: June 2018

Wildlife has often presented and suggested the effects of endocrine disrupting chemicals (EDCs). Animal studies have given us an important opportunity to understand the mechanisms of action of many chemicals on the endocrine system and on neurodevelopment and behaviour, and to evaluate the effects of doses, time and duration of exposure. Although results are sometimes conflicting because of confounding factors, epidemiological studies in humans suggest effects of EDCs on prenatal growth, thyroid function, glucose metabolism and obesity, puberty, fertility, and on carcinogenesis mainly through epigenetic mechanisms. This manuscript reviews the reports of a multidisciplinary national meeting on this topic.
Signal Transduction in Plant–Nematode Interactions
Authors: Ali Muhammad Amjad , Anjam Muhammad Shahzad , Nawaz Muhammad Amjad , Lam Hon-Ming , Chung Gyuhwa
Published: June 2018

To successfully invade and infect their host plants, plant parasitic nematodes (PPNs) need to evolve molecular mechanisms to overcome the defense responses from the plants. Nematode-associated molecular patterns (NAMPs), including ascarosides and certain proteins, while instrumental in enabling the infection, can be perceived by the host plants, which then initiate a signaling cascade leading to the induction of basal defense responses. To combat host resistance, some nematodes can inject effectors into the cells of susceptible hosts to reprogram the basal resistance signaling and also modulate the hosts’ gene expression patterns to facilitate the establishment of nematode feeding sites (NFSs). In this review, we summarized all the known signaling pathways involved in plant–nematode interactions. Specifically, we placed particular focus on the effector proteins from PPNs that mimic the signaling of the defense responses in host plants. Furthermore, we gave an updated overview of the regulation by PPNs of different host defense pathways such as salicylic acid (SA)/jasmonic acid (JA), auxin, and cytokinin and reactive oxygen species (ROS) signaling to facilitate their parasitic successes in plants. This review will enhance the understanding of the molecular signaling pathways involved in both compatible and incompatible plant–nematode interactions.
Mechanism of Salt-Induced Self-Compatibility Dissected by Comparative Proteomic Analysis in Brassica napus L.
Authors: Yang Yong , Liu Zhiquan , Zhang Tong , Zhou Guilong , Duan Zhiqiang , Li Bing , Dou Shengwei , Liang Xiaomei , Tu Jinxing , Shen Jinxiong , Yi Bin , Fu Tingdong , Dai Cheng , Ma Chaozhi
Published: June 2018

Self-incompatibility (SI) in plants genetically prevents self-fertilization to promote outcrossing and genetic diversity. Its hybrids in Brassica have been widely cultivated due to the propagation of SI lines by spraying a salt solution. We demonstrated that suppression of Brassica napus SI from edible salt solution treatment was ascribed to sodium chloride and independent of S haplotypes, but it did not obviously change the expression of SI-related genes. Using the isobaric tags for relative and absolute quantitation (iTRAQ) technique, we identified 885 differentially accumulated proteins (DAPs) in Brassica napus stigmas of un-pollinated (UP), pollinated with compatible pollen (PC), pollinated with incompatible pollen (PI), and pollinated with incompatible pollen after edible salt solution treatment (NA). Of the 307 DAPs in NA/UP, 134 were unique and 94 were shared only with PC/UP. In PC and NA, some salt stress protein species, such as glyoxalase I, were induced, and these protein species were likely to participate in the self-compatibility (SC) pathway. Most of the identified protein species were related to metabolic pathways, biosynthesis of secondary metabolites, ribosome, and so on. A systematic analysis implied that salt treatment-overcoming SI in B. napus was likely conferred by at least five different physiological mechanisms: (i) the use of Ca2+ as signal molecule; (ii) loosening of the cell wall to allow pollen tube penetration; (iii) synthesis of compatibility factor protein species for pollen tube growth; (iv) depolymerization of microtubule networks to facilitate pollen tube movement; and (v) inhibition of protein degradation pathways to restrain the SI response.