bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Maternal Exercise on Mice Offspring Development, Pulmonary Hypertension, and Vascular Remodeling in Chronic Hypoxia
Authors: LESLIE ERIC , GONZALEZ BOSC LAURA V. , SPECHT JONATHAN , MCKENNA ZACHARY J. , GRIDLEY REBEKAH , LUNA VINCENT , JONES DAVID T. , LANTZ BENJAMIN J. , MORIWAKI MIKA , HSIAO YU-YU , GIBSON ANN L. , MERMIER CHRISTINE , WILSON SEAN M. , DEYHLE MICHAEL R.
Published: May 2024

Purpose

Chronic, high-altitude hypoxic exposure increases the risk of high-altitude pulmonary hypertension (PH). Emerging evidence shows maternal exercise may improve offspring resistance to disease throughout life. The purpose of this study is to determine if maternal exercise mitigates chronic hypoxic-induced changes in the offspring indicative of high-altitude PH development.

Methods

Female adult C57BL/6J mice were randomly allocated to nonexercise or exercise conditions. Exercise consisted of voluntary running wheel exercise for 4 wk during the perinatal period. Three days after birth, the pups remained at low altitude (normoxia) or were exposed to hypobaric hypoxia of 450 mm Hg to simulate ~4500 m of altitude exposure until 8 wk of age. The study consisted of four groups: hypoxia + nonexercise pregnancy, hypoxia + exercise, or the respective normoxia conditions (normoxia + nonexercise or normoxia + exercise). Offspring body size, motor function, right ventricular systolic pressure (RVSP), and cardiopulmonary morphology were assessed after 8 wk in normoxia or hypoxia.

Results

Both hypoxic groups had smaller body sizes, reduced motor function, increased hematocrit, RVSP, muscularization in medium-sized pulmonary arteries, as well as right ventricular hypertrophy and contractility compared with the normoxic groups (P < 0.05).

Conclusions

Chronic hypoxia simulating 4500 m attenuated growth, lowered motor function, and elicited PH development. Voluntary maternal exercise did not significantly decrease RVSP in the offspring, which aligned with a lack of effect to attenuate abnormal body size and cardiopulmonary development due to chronic hypoxia. These findings are preliminary in nature, and more powered studies through larger group sizes are required to generalize the results to the population.
Genetic Liability to Cardiovascular Disease, Physical Activity, and Mortality: Findings from the Finnish Twin Cohort
Authors: JOENSUU LAURA , WALLER KATJA , KANKAANPÄÄ ANNA , PALVIAINEN TEEMU , KAPRIO JAAKKO , SILLANPÄÄ ELINA
Published: May 2024

Purpose

We investigated whether longitudinally assessed physical activity (PA) and adherence specifically to World Health Organization PA guidelines mitigate or moderate mortality risk regardless of genetic liability to cardiovascular disease (CVD). We also estimated the causality of the PA–mortality association.

Methods

The study used the older Finnish Twin Cohort with 4897 participants aged 33 to 60 yr (54.3% women). Genetic liability to coronary heart disease and systolic and diastolic blood pressure was estimated with polygenic risk scores (PRS) derived from the Pan-UK Biobank (N ≈ 400,000; >1,000,000 genetic variants). Leisure-time PA was assessed with validated and structured questionnaires three times during 1975 to 1990. The main effects of adherence to PA guidelines and the PRS × PA interactions were evaluated with Cox proportional hazards models against all-cause and CVD mortality. A cotwin control design with 180 monozygotic twin pairs discordant for meeting the guidelines was used for causal inference.

Results

During the 17.4-yr (mean) follow-up (85,136 person-years), 1195 participants died, with 389 CVD deaths. PRS (per 1 SD increase) were associated with a 17% to 24% higher CVD mortality risk but not with all-cause mortality except for the PRS for diastolic blood pressure. Adherence to PA guidelines did not show significant independent main effects or interactions with all-cause or CVD mortality. Twins whose activity levels adhered to PA guidelines over a 15-yr period did not have statistically significantly reduced mortality risk compared with their less active identical twin sibling. The findings were similar among high, intermediate, and low genetic risk levels for CVD.

Conclusions

The genetically informed Finnish Twin Cohort data could not confirm that adherence to PA guidelines either mitigates or moderates genetic CVD risk or causally reduces mortality risk.
Enhancing sustainable energy production through biomass gasification gas technology: a review
Authors: Alabi Oluwaseyi O. , Gbadeyan Oluwatoyin Joseph , Towoju Olumide A. , Deenadayalu Nirmala
Published: May 2024

This proposed research investigates the sustainable and innovative use of biomass gasification for generating electricity. Biomass gasification is a versatile and eco-friendly technology that converts organic materials, such as agricultural residues, forestry waste, and even municipal solid waste, into a valuable source of clean energy. This research delves into the various aspects of this technology, including its processes, efficiency, environmental impact, and potential applications in power generation. Biomass gasification gas, often referred to as syngas, presents a promising avenue for addressing the rising energy demand while lowering greenhouse gas emissions and preventing climate change. This research seeks to offer a thorough insight into the principles and practices behind biomass gasification, highlighting its role in the transition towards a sustainable and renewable energy future. The research will investigate the technical and economic feasibility of utilizing biomass gasification gas for electricity generation, examining the benefits, challenges, and opportunities associated with this alternative energy source. By addressing critical issues such as feedstock availability, gasifier technology, gas cleaning processes, and power plant integration, this study seeks to offer valuable insights into the potential of biomass gasification gas as a clean and renewable energy solution.
The attitude of the tsarist authorities towards railway – consequences for the Polish lands (until 1879). Jan Gottlieb Bloch’s “new” perspective
Authors: Pieczewski Andrzej
Published: May 2024

The article presents and analyses Jan Gottlieb Bloch’s views on the influence of tsarist rule on the development of the railway network in the Polish lands under Russian rule1 (up to 1879). The first part of the study is devoted to showing, in general, the tsars’ changing attitude towards the construction of railways in the Russian Empire. The specifics and conditions of railway construction in the Kingdom of Poland are presented. Bloch’s views and research results on the influence of the Russian authorities on the shape of the railway network in the Polish lands are then presented. Iron road projects submitted to the Russian authorities by domestic and foreign entrepreneurs, which did not receive approval, are listed and described. Thus, a picture emerges of “missed opportunities” – railway lines that would have been built had it not been for the specific policy of the Russian authorities. The conclusions of Bloch’s research do not contradict the common view in the literature on the negative influence of the tsarist authorities on the development of railways in the Polish lands under the Russian partition. Bloch’s research, however, goes a step further – it shows a measurable picture of the losses caused by the tsarist policy of a “railway desert”, particularly referring to Polish lands.
The drawing of borders in occupied territories of the former Polish-Lithuanian Commonwealth as an instrument of implementation of German special policies
Authors: Gierowska-Kałłaur Joanna
Published: May 2024

The work aims to present German political concepts implemented in the territories of the former Grand Duchy of Lithuania occupied by the Reich during World War I. Their main contractors were Chief of the General Staff Erich Ludendorff and Field Marshal Paul von Hindenburg. The key objective of this special policy was the maximum weakening of Poles’ self-organisational capabilities.
The German concept of “self-cleansing actions” (Selbstreinigungsaktionen) – formulation, implementation, oblivion, and suppression
Authors: Kuberski Hubert
Published: May 2024

Over a quarter of a century has passed since film documentary director Agnieszka Arnold discovered previously unknown information on the “self-cleansing action” in Jedwabne. The brief period of interest taken in this subject by the media and historians did not bring a thorough explanation of the “Jedwabne complex”. We still know little of how the Germans and Austrians forming part of Einsatzgruppen instigated and implemented, or instead inspired, genocidal Selbstrenigungsaktionen taking place in the rear area of all German army groups (Heeresgruppen). The Romanians adopted a slightly different strategy, although it, too, resulted in the extermination of the Jewish population in the territories recovered after their seizure by the Soviets in 1940. In popular understanding of the issue, the so-called pogroms (although this is not a correct term) occurred only in Podlasie/Podlachia/Podlachien region, Kovno/Kauen/Kaunas, and Lvov/Lemberg/Lviv. In reality, their scope spanned the entirety of the “Bloodlands”, from the General Government to the Caucasus. It is important to note that the term Selbstrenigungsaktionen is practically absent from scholarly discourse (suffice it to type it in an Internet search engine). It is worrying that this genocidal episode of the Shoah goes nearly unstudied – of course, as part of international research initiatives free from accusations of biased exploration.
Analysis of quality control on cassava emping “Super Telur Bu Siti” in Bantul Karang Yogyakarta
Authors: Timur Farradila Caesar Ratna , Fitriani Aprilia
Published: May 2024

Cassava emping is a snack made from mashed cassava mixed with eggs, red chilies, green onions, and celery leaves. This research aims to analyze the quality control of cassava emping products and efforts to improve the production process of "Super Telur Bu Siti" Cassava Emping. Data collection methods in this research are interviews, observation, and literature study. Several problems are caused by facts that need to conform to standards in similar food production processes. From the analysis using affinity diagrams, several factors cause quality problems, namely materials, machines, methods, humans, and the environment. The alternative tree diagram analysis results are efforts or actions to improve the quality of cassava chip products in "Super Telur Bu Siti" Cassava Emping. This research concludes that quality control is carried out based on aspects of raw materials, processes during processing, and final products. The Cassava Emping product "Super Telur Bu Siti" does not meet the quality standard criteria based on the National Standards and the Indonesian Food and Drug Supervisory Agency regarding Good Food Production Methods for Home Industries or CPPB-IRT 2012.
Diel Patterns Hold Promise as an Ecological Trait for Ants
Authors: Ohyama Leo , Booher Douglas B. , Lucky Andrea
Published: May 2024

Ecological traits have flourished in insect-based studies, resulting in a substantial and growing list of measurable traits. One trait that will likely become more attractive as data quality and curation improve is the diel patterns of insect activities. Diel patterns in ants can help better understand vital ecological processes such as competition and invasion biology. Because diel activity has the potential to be an informative trait in ants, we assessed the diel designations of foraging ants across the literature to quantify and assess the variation and sampling extent of this particular trait. We collected diel designations from 104 peer-reviewed scientific articles and quantified these data across important and documented ecological traits. We found that a disproportionate amount of solitary foraging ants were primarily diurnal foragers relative to ants that cooperatively forage. Our data show that diel patterns in foraging vary widely within and across ant genera. Importantly, we highlight the undersampling of this crucial ecological trait, which currently limits its utility. Our efforts highlight the importance of assessing an ecologically important trait’s landscape of reported data.
Mating Behavior of The Small Carpenter Ant, Camponotus yamaokai
Authors: Chiyoda Kazuma , Inoue Kanata , Nishisue Koji , Yamasaki Kazuhisa , Satoh Toshiyuki , Koyama Satoshi
Published: May 2024

Ants are known to show diverse mating behaviors across different species, but detailed reports on such behaviors are limited to only 0.7% of ant species. Therefore, we report on the mating behavior of the alate females and males in the small carpenter ant, Camponotus yamaokai. Its process was classified into two phases: mating attempt, and copulation. Out of 53 pairs we observed, 23 pairs engaged in a total of 36 mating attempts. The mating attempt durations in cases where copulation failed were significantly longer than those leading to copulation. Among these attempts, copulation was observed 10 times in 9 pairs, including multiple mating in one pair. The copulation lasted for 24.0 ± 5.1 seconds (mean ± SD). At the end of copulation, males pulled out their genitalia quickly to avoid potential attacks from the females. This behavior might be a strategy of males to minimize the risk of mortality during a single mating and support them to engage in multiple mating.
Geographical and temporal variations in the provision of registered older people long-term care home places in Wales
Authors: Higgs Gary , Langford Mitchel
Published: May 2024

Deep-seated problems facing the residential and nursing care home sector have been compounded by the impacts of the pandemic, underfunding and the cost-of-living crisis. This study aims to investigate how patterns of access to registered residential and nursing care places changed in the period spanning lockdowns and demonstrate how spatial analytical techniques can be used to examine the potential impacts of ongoing pressures on geographical patterns of access. Findings identify variations in access to provision hidden within aggregate figures that can help stakeholders monitor the current and projected availability of places as part of an overall package of care provision.
Development cooperation and the stratification of lesbian, gay, bi- and transsexual activism: international donors, elite activists and community members during Uganda Pride 2022
Authors: Svensson Jakob , Strand Cecilia
Published: May 2024

Uganda’s infamous state-sanctioned homo-hostility has resulted in intense international attention, development cooperation and Western funding to local lesbian, gay, bi- and transsexual (LGBT+) organisations. However, Western funders and allies in this context are becoming increasingly questioned. Researchers have highlighted the complexities, opportunities and constraints of an increasingly transnational LGBT+ movement, but how is this manifested on the ground in the Global South? Through an inductive and ethnographically inspired study, we set out to explore the Ugandan LGBT+ community and its intra-community relationships and relations with Western funders and allies in the unique setting of Uganda Pride 2022, to which we had rare first-hand access. The results reveal that security concerns, both from outside and within the community, shaped Uganda Pride 2022. The most salient finding is that competition for international funding distorts activists’ relations, as it stratifies the LGBT+ community based on who has access to Western donors and international funders.
Plasmonically engineered nitrogen-vacancy spin readout
Authors: Hapuarachchi Harini , Campaioli Francesco , Jelezko Fedor , Cole Jared H.
Published: May 2024

Ultra-precise readout of single nitrogen-vacancy (NV) spins holds promise for major advancements in quantum sensing, computing, and communication technologies. Here we present a rigorous open quantum theory capable of simultaneously capturing the optical, vibronic, and spin interactions of the negatively charged NV center, both in the presence and absence of plasmonic interaction. Our theory is verified against existing experiments in the literature. We predict orders of magnitude brightness and contrast enhancements in optically detected magnetic resonance (ODMR) and NV spin qubit readout arising from plasmonic interaction. Such optimal enhancements occur in carefully engineered parameter regions, necessitating rigorous modelling prior to experimentation. Our theory equips the community with a tool to identify such regions.
Application limits of the scaling relations for Monte Carlo simulations in diffuse optics. Part 2: results
Authors: Amendola Caterina , Maffeis Giulia , Farina Andrea , Spinelli Lorenzo , Torricelli Alessandro , Pifferi Antonio , Sassaroli Angelo , Fanelli Duccio , Tommasi Federico , Martelli Fabrizio
Published: May 2024

The limits of applicability of scaling relations to generate new simulations of photon migration in scattering media by re-scaling an existing Monte Carlo simulation are investigated both for the continuous wave and the time domain case. We analyzed the convergence properties in various scenarios by numerical methods, trying to derive practical guidelines for the judicious use of this approach, as well as a deeper understanding of the physics behind such relations. In the case of scaling of the absorption coefficient, the convergence is always rigorous both for the forward and inverse problems, relying on the derivatives with respect to the absorption coefficient. Also, the regenerated simulation inherits the very same noise of the original Monte Carlo simulation. In the case of scaling of the scattering coefficient, the situation is more critical. For forward problems, even for just a 10% uniform increase in scattering, appreciable deviations are observed whenever a high number of scattering interactions is involved. We tested a practical criterion based on the number of scattering events in the original simulation to judge the convergence of the scaling factors. For inverse problems, the scaling relations provide accurate regenerated simulations apart from the noise level that is increased with respect to the initial simulation, although anyway lower than the noise level obtained by implementing the direct calculation. The results of this study are important whenever an increase of Monte Carlo code throughput is mandatory, e.g., for fast data analysis of diffuse data, or in machine-learning scenarios, when generating huge datasets is needed.
Prior-free 3D tracking of a fast-moving object at 6667 frames per second with single-pixel detectors
Authors: Zhang Huan , Liu Zonghao , Zhou Mi , Zhang Zibang , Chen Muku , Geng Zihan
Published: May 2024

Real-time tracking and 3D trajectory computation of fast-moving objects is a promising technology, especially in the field of autonomous driving. However, existing image-based tracking methods face significant challenges when it comes to real-time tracking, primarily due to the limitation of storage space and computational resources. Here, we propose a novel approach that enables real-time 3D tracking of a fast-moving object without any prior motion information and at a very low computational cost. To enable 3D coordinate synthesis with a space-efficient optical setup, geometric moment patterns are projected on two non-orthogonal planes with a spatial resolution of 125 μm. Our experiment demonstrates an impressive tracking speed of 6667 frames per second (FPS) with a 20 kHz digital micromirror device (DMD), which is more than 200 times faster than the widely adopted video-based tracking methods. To the best of our knowledge, this is the highest tracking speed record in the field of single-pixel 3D trajectory tracking. This method promotes the development of real-time tracking techniques with single-pixel imaging (SPI).
Structured and disordered regions of Ataxin-2 contribute differently to the specificity and efficiency of mRNP granule formation
Authors: Petrauskas Arnas , Fortunati Daniel L. , Kandi Arvind Reddy , Pothapragada Sai Shruti , Agrawal Khushboo , Singh Amanjot , Huelsmeier Joern , Hillebrand Jens , Brown Georgia , Chaturvedi Dhananjay , Lee Jongbo , Lim Chunghun , Auburger Georg , VijayRaghavan K. , Ramaswami Mani , Bakthavachalu Baskar
Published: May 2024

Ataxin-2 (ATXN2) is a gene implicated in spinocerebellar ataxia type II (SCA2), amyotrophic lateral sclerosis (ALS) and Parkinsonism. The encoded protein is a therapeutic target for ALS and related conditions. ATXN2 (or Atx2 in insects) can function in translational activation, translational repression, mRNA stability and in the assembly of mRNP-granules, a process mediated by intrinsically disordered regions (IDRs). Previous work has shown that the LSm (Like-Sm) domain of Atx2, which can help stimulate mRNA translation, antagonizes mRNP-granule assembly. Here we advance these findings through a series of experiments on Drosophila and human Ataxin-2 proteins. Results of Targets of RNA Binding Proteins Identified by Editing (TRIBE), co-localization and immunoprecipitation experiments indicate that a polyA-binding protein (PABP) interacting, PAM2 motif of Ataxin-2 may be a major determinant of the mRNA and protein content of Ataxin-2 mRNP granules. Experiments with transgenic Drosophila indicate that while the Atx2-LSm domain may protect against neurodegeneration, structured PAM2- and unstructured IDR- interactions both support Atx2-induced cytotoxicity. Taken together, the data lead to a proposal for how Ataxin-2 interactions are remodelled during translational control and how structured and non-structured interactions contribute differently to the specificity and efficiency of RNP granule condensation as well as to neurodegeneration.
Augmenting complex and dynamic performance through mindfulness-based cognitive training: An evaluation of training adherence, trait mindfulness, personality and resting-state EEG
Authors: Dziego Chloe A. , Bornkessel-Schlesewsky Ina , Schlesewsky Matthias , Sinha Ruchi , Immink Maarten A. , Cross Zachariah R.
Published: May 2024

Human performance applications of mindfulness-based training have demonstrated its utility in enhancing cognitive functioning. Previous studies have illustrated how these interventions can improve performance on traditional cognitive tests, however, little investigation has explored the extent to which mindfulness-based training can optimise performance in more dynamic and complex contexts. Further, from a neuroscientific perspective, the underlying mechanisms responsible for performance enhancements remain largely undescribed. With this in mind, the following study aimed to investigate how a short-term mindfulness intervention (one week) augments performance on a dynamic and complex task (target motion analyst task; TMA) in young, healthy adults (n = 40, age range = 18–38). Linear mixed effect modelling revealed that increased adherence to the web-based mindfulness-based training regime (ranging from 0–21 sessions) was associated with improved performance in the second testing session of the TMA task, controlling for baseline performance. Analyses of resting-state electroencephalographic (EEG) metrics demonstrated no change across testing sessions. Investigations of additional individual factors demonstrated that enhancements associated with training adherence remained relatively consistent across varying levels of participants’ resting-state EEG metrics, personality measures (i.e., trait mindfulness, neuroticism, conscientiousness), self-reported enjoyment and timing of intervention adherence. Our results thus indicate that mindfulness-based cognitive training leads to performance enhancements in distantly related tasks, irrespective of several individual differences. We also revealed nuances in the magnitude of cognitive enhancements contingent on the timing of adherence, regardless of total volume of training. Overall, our findings suggest that mindfulness-based training could be used in a myriad of settings to elicit transferable performance enhancements.
Bioreactance assessment of cardiac output lacks reliability for the follow-up of patients with pulmonary hypertension
Authors: Turquier Ségolène , Huot Laure , Lamkhioued Medhi , Subtil Fabien , Traclet Julie , Ahmad Kais , Lestelle François , Chauvelot Louis , Cottin Vincent , Mornex Jean-François
Published: May 2024

Cardiac output (CO) is one of the primary prognostic factors evaluated during the follow-up of patients treated for pulmonary hypertension (PH). It is recommended that it be measured using the thermodilution technique during right heart catheterization. The difficulty to perform iterative invasive measurements on the same individual led us to consider a non-invasive option. The aims of the present study were to assess the agreement between CO values obtained using bioreactance (Starling™ SV) and thermodilution, and to evaluate the ability of the bioreactance monitor to detect patients whose CO decreased by more than 15% during follow-up and, accordingly, its usefulness for patient monitoring. A prospective cohort study evaluating the performance of the Starling™ SV monitor was conducted in patients with clinically stable PH. Sixty patients referred for hemodynamic assessment were included. CO was measured using both the thermodilution technique and bioreactance during two follow-up visits. A total of 60 PH patients were included. All datasets were available at the baseline visit (V0) and 50 of them were usable during the follow-up visit (V1). Median [IQR] CO was 4.20 l/min [3.60–4.70] when assessed by bioreactance, and 5.30 l/min [4.57–6.20] by thermodilution (p<0.001). The Spearman correlation coefficient was 0.51 [0.36–0.64], and the average deviation on Bland-Altman plot was -1.25 l/min (95% CI [-1.48–1.01], p<0.001). The ability of the monitor to detect a variation in CO of more than 15% between two follow-up measurements, when such variation existed using thermodilution, was insufficient for clinical practice (AUC = 0.54, 95% CI [0.33–0.75]).
FPNC Net: A hydrogenation catalyst image recognition algorithm based on deep learning
Authors: Hou Shichao , Zhao Peng , Cui Peng , Xu Hua , Zhang Jinrong , Liu Jian , An Mi , Lin Xinchen
Published: May 2024

The identification research of hydrogenation catalyst information has always been one of the most important businesses in the chemical industry. In order to aid researchers in efficiently screening high-performance catalyst carriers and tackle the pressing challenge at hand, it is imperative to find a solution for the intelligent recognition of hydrogenation catalyst images. To address the issue of low recognition accuracy caused by adhesion and stacking of hydrogenation catalysts, An image recognition algorithm of hydrogenation catalyst based on FPNC Net was proposed in this paper. In the present study, Resnet50 backbone network was used to extract the features, and spatially-separable convolution kernel was used to extract the multi-scale features of catalyst fringe. In addition, to effectively segment the adhesive regions of stripes, FPN (Feature Pyramid Network) is added to the backbone network for deep and shallow feature fusion. Introducing an attention module to adaptively adjust weights can effectively highlight the target features of the catalyst. The experimental results showed that the FPNC Net model achieved an accuracy of 94.2% and an AP value improvement of 19.37% compared to the original CenterNet model. The improved model demonstrates a significant enhancement in detection accuracy, indicating a high capability for detecting hydrogenation catalyst targets
The Temporary Incapacity (TI) register as a complementary system to traditional epidemiological surveillance during the COVID-19 pandemic in Spain
Authors: Lévano Dante Culqui , López Sofía Escalona , Gherasim Alín , Domínguez Jesús Oliva , Rico María Teresa Disdier , Gómez Montserrat García
Published: May 2024

Introduction

During the COVID-19 pandemic, a set of social measures were adopted for the preservation of business activity and the protection of workers. One of these measures was issuing the Temporary Disability (TD) for COVID-19 cases, close contacts, and especially vulnerable workers.

Objetive

This study analyzes whether the TD registry could be used as a complementary source to traditional epidemiological surveillance.

Methods

A longitudinal study of time series was carried out with a cross-correlation analysis of TD and COVID-19 cases reported to the National Epidemiological Surveillance Network (RENAVE). The analysis included six pandemic waves between 10/03/2020 and 31/12/2021 in Spain. Cross-correlation coefficients (r) were calculated using a time lag of -14 days.

Results

During the study period, 2,253,573 TD processes were recorded in Spain and 4,894,802 COVID-19 cases were reported to RENAVE. Significant positive correlations were observed at time lags of -7, -10, and -14, indicating that TD notification preceded RENAVE notification. In the first and sixth pandemic waves, TD notification preceded RENAVE by 12 and 7 days, respectively. Negative correlations between the two series were observed in the second and fourth waves, coinciding with a lower number of reported cases. In the third and fifth waves, TD notification also preceded RENAVE (lags -1, -5 and -14, -7, respectively).

Conclusions

The results confirm the usefulness of TD registry as a complementary system to traditional epidemiological surveillance in Spain, by detecting COVID-19 cases in the 7, 10, and 14 days prior. A better positive correlation is observed in waves where more cases were reported.
Transgender health objectives of training for adult Endocrinology and Metabolism programs: Outcomes of a modified-Delphi study
Authors: Ahmad Tehmina , Schreyer Leighton , Fung Raymond , Yu Catherine
Published: May 2024

Background

Transgender people encounter significant barriers when seeking timely, high-quality healthcare, resulting in unmet medical needs with increased rates of diabetes, asthma, chronic obstructive pulmonary disease, and HIV. The paucity of postgraduate medical education to invest in standardization of transgender health training sustains these barriers, leaving physicians feeling unprepared and averse to provide transgender health care. Closing this education gap and improving transgender healthcare necessitates the development of consensus-built transgender health objectives of training (THOOT), particularly in Adult Endocrinology and Metabolism Residency programs.

Methods

We conducted a two-round modified-Delphi process involving a nationally representative panel of experts, including Adult Endocrinology and Metabolism program directors, physician content experts, residents, and transgender community members, to identify THOOT for inclusion in Canadian Endocrinology and Metabolism Residency programs. Participants used a 5-point Likert scale to assess THOOT importance for curricular inclusion, with opportunities for written feedback. Data was collected through Qualtrics and analyzed after each round.

Findings

In the first Delphi round, panelists reviewed and rated 81 literature extracted THOOT, achieving consensus on all objectives. Following panelists’ feedback, 5 THOOT were added, 9 removed, 34 consolidated into 12 objectives, and 47 were rephrased or retained. In the second Delphi round, panelists assessed 55 THOOT. Consensus was established for 8 THOOT. Program directors’ post-Delphi feedback further consolidated objectives to arrive at 4 THOOT for curriculum inclusion.

Conclusions

To our knowledge, this is the first time a consensus-based approach has been used to establish THOOT for any subspecialty postgraduate medicine program across Canada or the United States. Our results lay the foundation towards health equity and social justice in transgender health medical education, offering a blueprint for future innovations.
Plastid phylogenomics and cytonuclear discordance in Rubioideae, Rubiaceae
Authors: Thureborn Olle , Wikström Niklas , Razafimandimbison Sylvain G. , Rydin Catarina
Published: May 2024

In this study of evolutionary relationships in the subfamily Rubioideae (Rubiaceae), we take advantage of the off-target proportion of reads generated via previous target capture sequencing projects based on nuclear genomic data to build a plastome phylogeny and investigate cytonuclear discordance. The assembly of off-target reads resulted in a comprehensive plastome dataset and robust inference of phylogenetic relationships, where most intratribal and intertribal relationships are resolved with strong support. While the phylogenetic results were mostly in agreement with previous studies based on plastome data, novel relationships in the plastid perspective were also detected. For example, our analyses of plastome data provide strong support for the SCOUT clade and its sister relationship to the remaining members of the subfamily, which differs from previous results based on plastid data but agrees with recent results based on nuclear genomic data. However, several instances of highly supported cytonuclear discordance were identified across the Rubioideae phylogeny. Coalescent simulation analysis indicates that while ILS could, by itself, explain the majority of the discordant relationships, plastome introgression may be the better explanation in some cases. Our study further indicates that plastomes across the Rubioideae are, with few exceptions, highly conserved and mainly conform to the structure, gene content, and gene order present in the majority of the flowering plants.
Zeaxanthin dipalmitate-enriched wolfberry extract improves vision in a mouse model of photoreceptor degeneration
Authors: Chen Xiongmin , Zhang Sensen , Yang Lili , Kong Qihang , Zhang Wenhua , Zhang Jinhong , Hao Xiangfeng , So Kwok-Fai , Xu Ying
Published: May 2024

Zeaxanthin dipalmitate (ZD) is a chemical extracted from wolfberry that protects degenerated photoreceptors in mouse retina. However, the pure ZD is expensive and hard to produce. In this study, we developed a method to enrich ZD from wolfberry on a production line and examined whether it may also protect the degenerated mouse retina. The ZD-enriched wolfberry extract (ZDE) was extracted from wolfberry by organic solvent method, and the concentration of ZD was identified by HPLC. The adult C57BL/6 mice were treated with ZDE or solvent by daily gavage for 2 weeks, at the end of the first week the animals were intraperitoneally injected with N-methyl-N-nitrosourea to induce photoreceptor degeneration. Then optomotor, electroretinogram, and immunostaining were used to test the visual behavior, retinal light responses, and structure. The final ZDE product contained ~30mg/g ZD, which was over 9 times higher than that from the dry fruit of wolfberry. Feeding degenerated mice with ZDE significantly improved the survival of photoreceptors, enhanced the retinal light responses and the visual acuity. Therefore, our ZDE product successfully alleviated retinal morphological and functional degeneration in mouse retina, which may provide a basis for further animal studies for possible applying ZDE as a supplement to treat degenerated photoreceptor in the clinic.
Identification of ligand and receptor interactions in CKD and MASH through the integration of single cell and spatial transcriptomics
Authors: Moreno Jaime , Gluud Lise Lotte , Galsgaard Elisabeth D. , Hvid Henning , Mazzoni Gianluca , Das Vivek
Published: May 2024

Background

Chronic Kidney Disease (CKD) and Metabolic dysfunction-associated steatohepatitis (MASH) are metabolic fibroinflammatory diseases. Combining single-cell (scRNAseq) and spatial transcriptomics (ST) could give unprecedented molecular disease understanding at single-cell resolution. A more comprehensive analysis of the cell-specific ligand-receptor (L-R) interactions could provide pivotal information about signaling pathways in CKD and MASH. To achieve this, we created an integrative analysis framework in CKD and MASH from two available human cohorts.

Results

The analytical framework identified L-R pairs involved in cellular crosstalk in CKD and MASH. Interactions between cell types identified using scRNAseq data were validated by checking the spatial co-presence using the ST data and the co-expression of the communicating targets. Multiple L-R protein pairs identified are known key players in CKD and MASH, while others are novel potential targets previously observed only in animal models.

Conclusion

Our study highlights the importance of integrating different modalities of transcriptomic data for a better understanding of the molecular mechanisms. The combination of single-cell resolution from scRNAseq data, combined with tissue slide investigations and visualization of cell-cell interactions obtained through ST, paves the way for the identification of future potential therapeutic targets and developing effective therapies.
Oculomotor behavior can be adjusted on the basis of artificial feedback signals indicating externally caused errors
Authors: Heins Frauke , Lappe Markus
Published: May 2024

Whether a saccade is accurate and has reached the target cannot be evaluated during its execution, but relies on post-saccadic feedback. If the eye has missed the target object, a secondary corrective saccade has to be made to align the fovea with the target. If a systematic post-saccadic error occurs, adaptive changes to the oculomotor behavior are made, such as shortening or lengthening the saccade amplitude. Systematic post-saccadic errors are typically attributed internally to erroneous motor commands. The corresponding adaptive changes to the motor command reduce the error and the need for secondary corrective saccades, and, in doing so, restore accuracy and efficiency. However, adaptive changes to the oculomotor behavior also occur if a change in saccade amplitude is beneficial for task performance, or if it is rewarded. Oculomotor learning thus is more complex than reducing a post-saccadic position error. In the current study, we used a novel oculomotor learning paradigm and investigated whether human participants are able to adapt their oculomotor behavior to improve task performance even when they attribute the error externally. The task was to indicate the intended target object among several objects to a simulated human-machine interface by making eye movements. The participants were informed that the system itself could make errors. The decoding process depended on a distorted landing point of the saccade, resulting in decoding errors. Two different types of visual feedback were added to the post-saccadic scene and we compared how participants used the different feedback types to adjust their oculomotor behavior to avoid errors. We found that task performance improved over time, regardless of the type of feedback. Thus, error feedback from the simulated human-machine interface was used for post-saccadic error evaluation. This indicates that 1) artificial visual feedback signals and 2) externally caused errors might drive adaptive changes to oculomotor behavior.
Association between red cell distribution width and hypertension: Results from NHANES 1999–2018
Authors: Chen Ying , Hou Xiaoxiao , Zhong Jiaxin , Liu Kai
Published: May 2024

The relationship between red cell distribution width (RDW) and hypertension remains a contentious topic, with a lack of large-scale studies focusing on the adults in the United States. This study aimed to investigate the association between RDW and hypertension among US adults from 1999 to 2018. Methods: Data were derived from the National Health and Nutrition Examination Survey (NHANES) 1999–2018. RDW values were obtained from the Laboratory Data’s Complete Blood Count with 5-part Differential—Whole Blood module. Hypertension data were obtained through hypertension questionnaires and blood pressure measurements. Multivariable weighted logistic regression analyses were conducted to assess the association between RDW and hypertension, followed by subgroup and smooth curve analyses. Results: Compared to the non-hypertensive group, the hypertensive group exhibited higher RDW values (13.33±1.38 vs. 12.95±1.27, P <0.001). After adjusting for covariates, weighted multivariable logistic regression analysis revealed a positive correlation between RDW and hypertension prevalence (OR: 1.17, 95% CI 1.13, 1.21, P <0.001). When RDW was included as a categorical variable, participants in the fourth quartile had the highest risk of hypertension (OR: 1.86, 95% CI 1.70, 2.03, P <0.001). Subgroup analysis showed that, except for age, BMI and weak/failing kidneys, gender, race, education level, smoking, alcohol use, congestive heart failure, and stroke did not significantly influence this correlation (all P-values for interaction >0.05).Smooth curve fitting analysis revealed a reverse J-shaped relationship between RDW and hypertension prevalence, with an inflection point at 12.93%. Conclusion: We first explored the relationship between RDW and hypertension among US adults and discovered a reverse J-shaped association, providing further insights into the relationship between blood cell counts and hypertension and offering a new foundation for hypertension prevention and control.
Perinatal mortality after Chornobyl in contaminated regions of Ukraine
Authors: Körblein Alfred
Published: May 2024

Background

Belarus and Ukraine were the countries most affected by the consequences of the Chornobyl nuclear power plant in 1986. A study of perinatal mortality in Belarus found a highly statistically significant increase in the 1990s in the most contaminated oblast Gomel but no increase during this period in the rest of Belarus. As a possible mechanism to interpret this increase as a late Chornobyl effect, it has been suggested that strontium-90 contained in Chornobyl fallout, incorporated during menarche, impairs the immune system of pregnant women which in turn increases perinatal mortality. In the present study, this hypothesis is tested using data from Ukraine.

Methods

Annual data on perinatal mortality, in the period 1981–2006 were provided by the Ministry of Public Health of Ukraine. Trends in perinatal mortality rates in the most contaminated regions of Ukraine (Kyiv and Zhytomyr oblasts and the city of Kyiv; study region) were compared with rates in the rest of Ukraine (control region). To identify any differences in perinatal mortality trends between the study and control regions, the ratios of perinatal mortality rates in the study region to the rates in the control region were analyzed using the calculated strontium concentration as a predictor.

Results

A trend analysis of perinatal mortality rates in Ukraine revealed two bell-shaped deviations from a long-term exponential trend with maxima at the beginning and end of the 1990s. The same pattern was found in the data from the study and control regions, but the deviations were almost three times higher in the study region than in the control region. An analysis of the ratios of perinatal mortality rates in the study region to the rates in the control region (odds ratios) showed an increase and decrease during the 1990s which was approximated by a lognormal density distribution. The calculated strontium concentration, when used as a predictor, also fitted the data well. Thus, the data from Ukraine confirms the results from Belarus. The analysis of the odds ratios revealed about 1000 excess perinatal deaths in the study region in the period 1990–2004. The corresponding figure for Ukraine as a whole was estimated at 3500 perinatal deaths.

Conclusion

It is hypothesized that the observed increase in perinatal mortality in the 1990s may be a late effect of incorporated strontium-90 on the immune system of pregnant women. The analysis is based on a theoretical model, as no data on strontium concentrations were available; the results should therefore be interpreted with caution. An updated study for Belarus would be desirable to corroborate the results.
Fungal burden, dimorphic transition and candidalysin: Role in Candida albicans-induced vaginal cell damage and mitochondrial activation in vitro
Authors: Spaggiari Luca , Ardizzoni Andrea , Ricchi Francesco , Pedretti Natalia , Squartini Ramos Caterina Alejandra , Squartini Ramos Gianfranco Bruno , Kenno Samyr , De Seta Francesco , Pericolini Eva
Published: May 2024

Candida albicans (C. albicans) can behave as a commensal yeast colonizing the vaginal mucosa, and in this condition is tolerated by the epithelium. When the epithelial tolerance breaks down, due to C. albicans overgrowth and hyphae formation, the generated inflammatory response and cell damage lead to vulvovaginal candidiasis (VVC) symptoms. Here, we focused on the induction of mitochondrial reactive oxygen species (mtROS) in vaginal epithelial cells after C. albicans infection and the involvement of fungal burden, morphogenesis and candidalysin (CL) production in such induction. Bioluminescent (BLI) C. albicans, C. albicans PCA-2 and C. albicans 529L strains were employed in an in vitro infection model including reconstituted vaginal epithelium cells (RVE), produced starting from A-431 cell line. The production of mtROS was kinetically measured by using MitoSOX™ Red probe. The potency of C. albicans to induced cell damage to RVE and C. albicans proliferation have also been evaluated. C. albicans induces a rapid mtROS release from vaginal epithelial cells, in parallel with an increase of the fungal load and hyphal formation. Under the same experimental conditions, the 529L C. albicans strain, known to be defective in CL production, induced a minor mtROS release showing the key role of CL in causing epithelial mithocondrial activation. C. albicans PCA-2, unable to form hyphae, induced comparable but slower mtROS production as compared to BLI C. albicans yeasts. By reducing mtROS through a ROS scavenger, an increased fungal burden was observed during RVE infection but not in fungal cultures grown on abiotic surface. Collectively, we conclude that CL, more than fungal load and hyphae formation, seems to play a key role in the rapid activation of mtROS by epithelial cells and in the induction of cell-damage and that mtROS are key elements in the vaginal epithelial cells response to C. albicans.
Factors associated with oral health care behavior of people with type 2 diabetes mellitus: A hospital-based, cross-sectional study
Authors: Sairat Kamonchanok , Phoosuwan Nitikorn
Published: May 2024

Background

Oral healthcare behavior determines oral health status and the incidence of oral diseases. People with type 2 diabetes mellitus (T2DM) are at-risk of having low oral healthcare behavior and disease-related oral health.

Objective

To investigate the oral health status and factors associated with oral healthcare behavior among people with T2DM in Thailand.

Methods

In total, 401 people with T2DM participated in the study based on their attendance at a non-communicable disease clinic at sub-district health promotion hospitals in Bueng Kan, a north-eastern province in Thailand. A structured questionnaire was used to obtain variables of interest. Linear regression analysis at the 95% confidence interval (CI) was applied.

Results

The majority of participants were female (73.8%). More than three-quarters had more than 20 permanent teeth (77.6%), a decay missing filling tooth index was 10.6 teeth/person. Many participants had four permanent occlusal pairs (69.6%), had tooth decay (74.6%), and some participants had tooth filling (32.2%). Statistically significant factors associated with oral healthcare behavior were: having complications associated with diabetes mellitus (Beta = -0.097, 95%CI = -1.653, -0.046), oral health literacy (Beta = 0.119, 95%CI = 0.009, 0.150), educational level (Beta = 0.123, 95%CI = 0.103, 0.949), oral healthcare attitude (Beta = 0.258, 95%CI = 0.143, 0.333), and oral health services (Beta = 0.430, 95%CI = 0.298, 1.408).

Conclusions

People with T2DM had good oral health status. People with T2DM with low oral health literacy, low attitude, and low level of oral health services were at a higher risk of poor oral healthcare behavior.