bluuebunny/crossref_metadata_2025 huggingface dataset:
Information Capacity of Positron Emission Tomography Scanners
Authors: Michail Christos , Karpetas George , Kalyvas Nektarios , Valais Ioannis , Kandarakis Ioannis , Agavanakis Kyriakos , Panayiotakis George , Fountos George
Published: December 2018

Background: The aim of the present study was to assess the upper information content bound of positron emission tomography (PET) images, by means of the information capacity (IC). Methods: The Geant4 Application for the Tomographic Emission (GATE) Monte Carlo (MC) package was used, and reconstructed images were obtained by using the software for tomographic image reconstruction (STIR). The case study for the assessment of the information content was the General Electric (GE) Discovery-ST PET scanner. A thin-film plane source aluminum (Al) foil, coated with a thin layer of silica and with a 18F-fludeoxyglucose (FDG) bath distribution of 1 MBq was used. The influence of the (a) maximum likelihood estimation-ordered subsets-maximum a posteriori probability-one step late (MLE-OS-MAP-OSL) algorithm, using various subsets (1 to 21) and iterations (1 to 20) and (b) different scintillating crystals on PET scanner’s performance, was examined. The study was focused on the noise equivalent quanta (NEQ) and on the single index IC. Images of configurations by using different crystals were obtained after the commonly used 2-dimensional filtered back projection (FBP2D), 3-dimensional filtered back projection re-projection (FPB3DRP) and the (MLE)-OS-MAP-OSL algorithms. Results: Results shown that the images obtained with one subset and various iterations provided maximum NEQ values, however with a steep drop-off after 0.045 cycles/mm. The single index IC data were maximized for the range of 8–20 iterations and three subsets. The PET scanner configuration incorporating lutetium orthoaluminate perovskite (LuAP) crystals provided the highest NEQ values in 2D FBP for spatial frequencies higher than 0.028 cycles/mm. Bismuth germanium oxide (BGO) shows clear dominance against all other examined crystals across the spatial frequency range, in both 3D FBP and OS-MAP-OSL. The particular PET scanner provided optimum IC values using FBP3DRP and BGO crystals (2.4829 bits/mm2). Conclusions: The upper bound of the image information content of PET scanners can be fully characterized and further improved by investigating the imaging chain components through MC methods.
Using Recurrent Procedures in Adaptive Control System for Identify the Model Parameters of the Moving Vessel on the Cross Slipway
Authors: Rudakova Hanna , Polyvoda Oksana , Omelchuk Anton
Published: December 2018

The article analyses the problems connected with ensuring the coordinated operation of slipway drives that arise during the launch of a ship. The dynamic model of load of the electric drive of the ship’s cart is obtained taking into account the peculiarities of the construction of the ship-lifting complex, which allows us to analyse the influence of external factors and random influences during the entire process of launching the ship. A linearized mathematical model of the dynamics of a complex vessel movement in the process of descent in the space of states is developed, which allows us to identify the mode of operation of the multi-drive system, taking into account its structure. The analysis of application efficiency of recurrent methods for identification (stochastic approximation and least squares) of the linearized model parameters in the space of states is carried out. A decision support system has been developed in the automated system of operational control by the module for estimating the situation and the control synthesis to ensure a coherent motion of a complex ship-carts object in a two-phase environment.
SolarView: Georgia Solar Adoption in Context
Authors: Tidwell Jacqueline Hettel , Tidwell Abraham , Nelson Steffan , Hill Marcus
Published: December 2018

The local-national gap is a problem currently plaguing the adoption of emerging technologies targeted at resolving energy transition issues that are characterized by disparities in the adoption of innovations and policies on a local level in response to national policy implementation. These disparities reflect a complex system of technical, economic, social, political, and ecological factors linked to the perceptions held by communities and how they see energy development and national/global policy goals. This dataset is an attempt to bridge the local-national gap regarding solar PV adoption in the State of Georgia (U.S.) by aggregating variables from seven different publicly-available sources. The objective of this activity was to design a resource that would help researchers interested in the context underlying solar adoption on the local scale of governance (e.g., the county level). The SolarView database includes information necessary for informing policy-making activities such as solar installation information, a historical county zip code directory, county-level census data, housing value indexes, renewable energy incentive totals, PV rooftop suitability percentages, and utility rates. As this is a database from multiple sources, incomplete data entries are noted.
Assessment of Residents’ Exposure to Leisure Noise in Málaga (Spain)
Authors: Asensio Cesar , Gasco Luis , De Arcas Guillermo , López Juan Manuel , Alonso Jesus
Published: December 2018

Leisure noise is a continual source of complaints from residents of affected areas, and its management poses a difficult challenge for local authorities, especially in tourist destinations, such as Málaga. The city council of this city has set a goal of mitigating the noise produced by leisure activities. In 2015, it began mitigation actions, starting with the assessment of the noise through a temporal monitoring campaign in two specific areas of the city where leisure activities are prevalent. Beyond the objective assessment of the noise levels through measurements, the research team programmed several communication actions (a) to improve the trust, visibility, and reliability of the noise-monitoring process through press and social networks and (b) to gather the subjective response to noise from residents in the affected areas. The results obtained were very helpful to raise awareness among stakeholders and to support the planning and prioritization of further noise mitigation actions. Furthermore, the research team aimed to analyze the long-term noise indicators and the time-based patterns of noise in different areas, trying to establish conclusions that can be helpful for other areas of the city and testing the applicability of previous leisure noise models for the city of Málaga. The results showed that the noise levels in the leisure areas in Málaga are quite high at night (Ln over 60 dBA in almost every location), especially during weekend nights, where we observed locations with noise levels over 75–80 dBA until late hours of the night. We also made an analysis of the leisure noise models proposed in previous investigations and their performance in the case of Málaga.
This Is How We Do It: How Social Norms and Social Identity Shape Decision Making under Uncertainty
Authors: Lipari Francesca
Published: December 2018

The current study aims to investigate how the presence of social norms defines belief formation on future changes in social identity (i.e., diachronic identity), and how those beliefs affect individual decisions under uncertainty. The paper proposes a theoretical model in which individuals have preferences over their own attributes and over specific information structures. The individual preferences are motivated by the presence of social norms. The norms, while establishing the socially acceptable attributes of an individual identity, also drive individuals’ preferences for information acquisition or avoidance. The model incorporates social norms as empirical expectations and provides a prior dependent theory that allows for prior-dependent information attitudes. Firstly, the model implies that decisions are mitigated by socially grounded behavioral and cognitive biases; and secondly, that it can create an incentive to avoid information, even when the latter is useful, free, and independent of strategic considerations. These biases bring out individual trade-offs between the accuracy of decision making and self-image motivated by social conformity. The two behavioral motivations are represented through a game of an intra-personal model of choice under uncertainty in which self-deception and memory manipulation mechanisms are used to overcome the individuals’ internal trade-off.
Repetitive Fragile Sites: Centromere Satellite DNA as a Source of Genome Instability in Human Diseases
Authors: Black Elizabeth M. , Giunta Simona
Published: December 2018

Maintenance of an intact genome is essential for cellular and organismal homeostasis. The centromere is a specialized chromosomal locus required for faithful genome inheritance at each round of cell division. Human centromeres are composed of large tandem arrays of repetitive alpha-satellite DNA, which are often sites of aberrant rearrangements that may lead to chromosome fusions and genetic abnormalities. While the centromere has an essential role in chromosome segregation during mitosis, the long and repetitive nature of the highly identical repeats has greatly hindered in-depth genetic studies, and complete annotation of all human centromeres is still lacking. Here, we review our current understanding of human centromere genetics and epigenetics as well as recent investigations into the role of centromere DNA in disease, with a special focus on cancer, aging, and human immunodeficiency–centromeric instability–facial anomalies (ICF) syndrome. We also highlight the causes and consequences of genomic instability at these large repetitive arrays and describe the possible sources of centromere fragility. The novel connection between alpha-satellite DNA instability and human pathological conditions emphasizes the importance of obtaining a truly complete human genome assembly and accelerating our understanding of centromere repeats’ role in physiology and beyond.
Mechanisms of DNA Methyltransferase Recruitment in Mammals
Authors: Laisné Marthe , Gupta Nikhil , Kirsh Olivier , Pradhan Sriharsa , Defossez Pierre-Antoine
Published: December 2018

DNA methylation is an essential epigenetic mark in mammals. The proper distribution of this mark depends on accurate deposition and maintenance mechanisms, and underpins its functional role. This, in turn, depends on the precise recruitment and activation of de novo and maintenance DNA methyltransferases (DNMTs). In this review, we discuss mechanisms of recruitment of DNMTs by transcription factors and chromatin modifiers—and by RNA—and place these mechanisms in the context of biologically meaningful epigenetic events. We present hypotheses and speculations for future research, and underline the fundamental and practical benefits of better understanding the mechanisms that govern the recruitment of DNMTs.
Receive Beam-Steering and Clutter Reduction for Imaging the Speed-of-Sound Inside the Carotid Artery
Authors: Kuriakose Maju , Muller Jan-Willem , Stähli Patrick , Frenz Martin , Jaeger Michael
Published: December 2018

Handheld imaging of the tissue’s speed-of-sound (SoS) is a promising multimodal addition to diagnostic ultrasonography for the examination of tissue composition. Computed ultrasound tomography in echo mode (CUTE) probes the spatial distribution of SoS, conventionally via scanning the tissue under a varying angle of ultrasound transmission, and quantifying—in a spatially resolved way—phase variations of the beamformed echoes. So far, this technique is not applicable to imaging the lumen of vessels, where blood flow and tissue clutter inhibit phase tracking of the blood echoes. With the goal to enable the assessment of atherosclerotic plaque composition inside the carotid artery, we propose two modifications to CUTE: (a) use receive (Rx) beam-steering as opposed to transmit (Tx) beam-steering to increase acquisition speed and to reduce flow-related phase decorrelation, and (b) conduct pairwise subtraction of data obtained from repetitions of the scan sequence, to highlight blood echoes relative to static echo clutter and thus enable the phase tracking of blood echoes. These modifications were tested in a phantom study, where the echogenicity of the vessel lumen was chosen to be similar to the one of the background medium, which allows a direct comparison of SoS images obtained with the different techniques. Our results demonstrate that the combination of Rx-steering with the subtraction technique results in an SoS image of the same quality as obtained with conventional Tx-steering. Together with the improved acquisition speed, this makes the proposed technique a key step towards successful imaging of the SoS inside the carotid artery.
Biosynthesis and Secretion of Human Tissue Kallikrein in Transgenic Chlamydomonas reinhardtii
Authors: Chen Jun , Wu Jinxia , Wu Qingyu , Hu Zhangli
Published: December 2018

The green alga Chlamydomonas reinhardtii was recently been shown to be an effective bio-manufacturing platform for the production of recombinant proteins. The advantage of using C. reinhardtii is that it is fast to grow, inexpensive to culture, and relatively safe. However, the expression of foreign proteins is always low and difficult to purify in C. reinhardtii. Human kallikrein has the potential to be developed into certain drugs, like insulin. Therefore, its biosynthesis is important to drug development. In this study, we synthesized the sg gene, a signal peptide sequence of alkaline phosphatase, and inserted it into a pH124 plasmid, which contains a HSP70A-RBCS2 promoter and a RBCS2 terminator. Then, we inserted the human kallikrein gene klk1 behind the sg sequence to make a pHsgk124 vector. The pHsgk124 were transferred into a cell-wall deficient strain of C. reinhardtii, cc-503, by using the glass bead method. Southern blot analysis showed that sg and klk1 were incorporated into genes of the transgenic C. reinhardtii. RT-PCR analysis showed that it had an active transcription and its expression increased three times under heat stress. Western blot analyses of proteins inside and outside cells (in the culture medium) showed that klk1 was expressed in the cell and the resulting protein was secreted into medium. An enzyme activity assay showed that the recombinant protein had the ability to hydrolyze the specific substrate H-D-Val-Leu-Arg-Pna. In conclusion, we successfully bioengineered C. reinhardtii to produce and secrete human kallikrein protein, which has important biomedical implications.
Effects of Maternal Decisional Authority and Media Use on Vaccination for Children in Asian Countries
Authors: Sohn Minsung , Lin Leesa , Jung Minsoo
Published: December 2018

Background and objectives: It is now accepted that vaccination is a critical public health strategy in preventing child morbidity and mortality. Understanding factors that promote vaccination is a critical first step. The objective of this study was to investigated associations of maternal decisional authority and media use on vaccination for children in six South and Southeast Asian countries. Materials and Methods: Data come from demographic and health surveys conducted in Bangladesh, Cambodia, Indonesia, Nepal, Pakistan, and the Philippines between 2011 and 2014 (N = 45,168 women). Main outcome variables were four types of basic vaccination for children. Independent variables were maternal decisional authority and media use. Hierarchical multivariable regression analyses were performed to examine associations. Results: Children of mothers who had more decisional authority were more likely to be vaccinated compared to those participants who did not have such authority. The likelihood to have their children vaccinated was higher among women who frequently used media than those who did not use media. Conclusions: Maternal decisional authority and media use are related to improved vaccination for children. To increase vaccination rates in developing countries in South and Southeast Asia, programs and policies that promote maternal decisional authority and the use of media for health need to be implemented to help families and local communities.
Evaluation Study on Iterative Inverse Modeling Procedure for Determining Post-Necking Hardening Behavior of Sheet Metal at Elevated Temperature
Authors: Mei Han , Lang Lihui , Liu Kangning , Yang Xiaoguang
Published: December 2018

The identification of the post-necking strain hardening behavior of metal sheet is important for finite element analysis procedures of sheet metal forming process. The inverse modeling method is a practical way to determine the hardening curve to large strains. This study is thus focused on the evaluation of the inverse modeling method using a novel material performance test. In this article, hot uniaxial tensile test of a commercially pure titanium sheet with rectangular section was first conducted. Utilizing the raw data from the tensile test, the post-necking hardening behavior of the material is determined by a FE-based inverse modeling procedure. Then the inverse method is compared with some classical hardening models. In order to further evaluate the applicability of the inverse method, biaxial tensile test at elevated temperatures was performed using a special designed cruciform specimen. The cruciform specimen could guarantee that the maximum equi-biaxial deformation occurs in the center section. By using the inverse modeling procedure, the hardening curves under biaxial stress state are able to be extracted. Finally the stress-strain curves obtained from the two experiments are compared and analysis studies are provided.
Recent Advances in AIV Biosensors Composed of Nanobio Hybrid Material
Authors: Lee Taek , Ahn Jae-Hyuk , Park Sun Yong , Kim Ga-Hyeon , Kim Jeonghyun , Kim Tae-Hyung , Nam Inho , Park Chulhwan , Lee Min-Ho
Published: December 2018

Since the beginning of the 2000s, globalization has accelerated because of the development of transportation systems that allow for human and material exchanges throughout the world. However, this globalization has brought with it the rise of various pathogenic viral agents, such as Middle East respiratory syndrome coronavirus (MERS-CoV), severe acute respiratory syndrome coronavirus (SARS-CoV), Zika virus, and Dengue virus. In particular, avian influenza virus (AIV) is highly infectious and causes economic, health, ethnical, and social problems to human beings, which has necessitated the development of an ultrasensitive and selective rapid-detection system of AIV. To prevent the damage associated with the spread of AIV, early detection and adequate treatment of AIV is key. There are traditional techniques that have been used to detect AIV in chickens, ducks, humans, and other living organisms. However, the development of a technique that allows for the more rapid diagnosis of AIV is still necessary. To achieve this goal, the present article reviews the use of an AIV biosensor employing nanobio hybrid materials to enhance the sensitivity and selectivity of the technique while also reducing the detection time and high-throughput process time. This review mainly focused on four techniques: the electrochemical detection system, electrical detection method, optical detection methods based on localized surface plasmon resonance, and fluorescence.
Manufacturing Signatures of Injection Molding and Injection Compression Molding for Micro-Structured Polymer Fresnel Lens Production
Authors: Loaldi Dario , Quagliotti Danilo , Calaon Matteo , Parenti Paolo , Annoni Massimiliano , Tosello Guido
Published: December 2018

Injection compression molding (ICM) provides enhanced optical performances of molded polymer optics in terms of birefringence and transmission of light compared to Injection molding (IM). Nevertheless, ICM requires case-dedicated process optimization to ensure that the required high accuracy geometrical replication is achieved, particularly especially in the case of surface micro-features. In this study, two factorial designs of experiments (DOE) were carried out to investigate the replication capability of IM and ICM on a micro structured Fresnel lens. A laser scanning confocal microscope was employed for the quality control of the optical components. Thus, a detailed uncertainty budget was established for the dimensional measurements of the replicated Fresnel lenses, considering specifically peak-to-valley (PV) step height and the pitch of the grooves. Additional monitoring of injection pressure allowed for the definition of a manufacturing signature, namely, the process fingerprint for the evaluation of the replication fidelity under different process conditions. Moreover, considerations on the warpage of parts were related to a manufacturing signature of the molding processes. At last, the global part mass average and standard deviation were measured to correlate local geometrical replication performances with global part quality trends.
Using Factor Analysis to Determine the Interrelationships between the Engineering Properties of Aggregates from Igneous Rocks in Greece
Authors: Giannakopoulou Panagiota P. , Petrounias Petros , Tsikouras Basilios , Kalaitzidis Stavros , Rogkala Aikaterini , Hatzipanagiotou Konstantin , Tombros Stylianos F.
Published: December 2018

This paper investigates the interrelationships between the engineering properties of igneous aggregate rocks from Greece with the aid of the R-mode factor analysis. The collected samples represent mafic and ultramafic rocks from the ophiolite complexes of Gerania, Guevgueli, Veria-Naousa, and Edessa as well as intermediate-acidic rocks from the surrounding areas of the complexes. Factor analysis verifies the important interdependences among the engineering parameters like physical, mechanical, geometrical, and physicochemical properties by giving statistical significance. Variations of the petrographic characteristics of the investigated rocks influence their engineering properties as well as the interdependence among them. Factor 1, which is the most representative one (~36% of the total variance), shows interdependences between certain physical, mechanical, physicochemical properties such as total porosity (nt) with moisture content (w), nt with the Los Abrasion value (LA), and the uniaxial compressive strength (UCS) with point load index Is(50). Additionally, the second factor (~27% of the total data variability) correlates physical properties such as w, nt, physicochemical properties such as the methylene blue test (MBF), mechanical properties such as UCS, Is(50), and loss on ignition (LOI), which highlights the effect of mineralogy on these properties. Lastly, Factor 3 (~14% of the total data variability) expresses the interdependence of the flakiness index (IF), which is an elongation index (IE) relative to their alteration (LOI).
A Novel Method to Limit the Adverse Effect of Fine Serpentine on the Flotation of Pyrite
Authors: Liu Dezhi , Zhang Guofan , Chen Yanfei , Chen Wei , Gao Yawen
Published: December 2018

A novel method to limit the adverse effect of fine serpentine on the flotation of pyrite was investigated in this paper. The flotation results showed that coarser serpentine possessed a weaker depression effect on the pyrite flotation process, and the use of KAl(SO4)2·12H2O could efficiently limit the detrimental effect of fine serpentine on pyrite with a maximum increase of pyrite recovery from 14% to 86% at pH 9.0. The results of particle size measurements and rheological measurements exhibited that the addition of KAl(SO4)2·12H2O increased the particle size of serpentine buta hrdly affected the particle size of pyrite, then limited the formation of serpentine-pyrite aggregates. Adsorption test results showed that the adsorption density of potassium butyl xanthate (PBX) onto pyrite regained with the addition of KAl(SO4)2·12H2O, thereby achieving good flotation improvement. It can be concluded that KAl(SO4)2·12H2O is likely to be an effective pyrite flotation reagent, especially in the presence of fine serpentine.
UPLC-MS/MS of Atractylenolide I, Atractylenolide II, Atractylenolide III, and Atractyloside A in Rat Plasma after Oral Administration of Raw and Wheat Bran-Processed Atractylodis Rhizoma
Authors: Xu Shizhao , Qi Xiaojie , Liu Yuqiang , Liu Yuhan , Lv Xin , Sun Jianzhi , Cai Qian
Published: December 2018

Atractylodis Rhizoma is the dried rhizome of Atractylodes lancea (Thunb.) DC. or Atractylodes chinensis (DC.) Koidz and is often processed by stir-frying with wheat bran to reduce its dryness and increase its spleen tonifying activity. However, the mechanism by which the processing has this effect remains unknown. To explain the mechanism based on the pharmacokinetics of the active compounds, a rapid, sensitive ultra-performance liquid chromatography-tandem mass spectrometry method was developed to analyze atractylenolides I, II, and III, and atractyloside A simultaneously in rat plasma after oral administration of raw and processed Atractylodis Rhizoma. Acetaminophen was used as the internal standard and the plasma samples were pretreated with methanol. Positive ionization mode coupled with multiple reaction monitoring mode was used to analyze the four compounds. The method validation revealed that all the calibration curves displayed good linear regression over the concentration ranges of 3.2–350, 4–500, 4–500, and 3.44–430 ng/mL for atractylenolides I, II, and III, and atractyloside A, respectively. The relative standard deviations of the intra- and inter-day precisions of the four compounds were less than 6% with accuracies (relative error) below 2.38%, and the extraction recoveries were more than 71.90 ± 4.97%. The main pharmacokinetic parameters of the four compounds were estimated with Drug and Statistics 3.0 and the integral pharmacokinetics were determined based on an area under the curve weighting method. The results showed that the integral maximum plasma concentration and area under the curve increased after oral administration of processed Atractylodis Rhizoma.
Supercritical CO2 Extraction of Eruca sativa Using Cosolvents: Phytochemical Composition by LC-MS Analysis
Authors: Sut Stefania , Boschiero Irene , Solana Miriam , Malagoli Mario , Bertucco Alberto , Dall’Acqua Stefano
Published: December 2018

Background: Eruca sativa Mill. is a good source of glucosinolates (GLS), phenolic compounds and unsaturated fatty acids, being a valuable material for the production of functional-foods or nutraceutical ingredients. Extraction by supercritical CO2 (SCO2) can be used and the limitations due to the apolar nature of CO2 can be overcome using co-solvents. In this paper different cosolvents and conditions were used for SCO2 extraction and the composition of the obtained extracts was studied by LC-MS. Results: Water resulted the ideal co-solvent, allowing the extraction of glucosinolates in comparable amounts to the classical procedure with boiling water, as it can be carried out at mild temperatures (45 °C vs. >100 °C). Increasing the pressure improved the GLS extraction. On the other hand polyphenol extraction under the studied conditions was not influenced by pressure and temperature variations. The in vitro antioxidant effect of the obtained extracts was also measured, showing significant activity in the DPPH and FC tests. Conclusions: The GLS, flavonoids and lipids composition of the obtained extracts was studied, showing the presence of numerous antioxidant constituents useful for nutraceutical applications. The extraction method using SCO2 and water as co-solvent presents advantages in terms of safety because these solvents are generally recognised as safe. Water as cosolvent at 8% resulted useful for the extraction of both glucosinolates and phenolics in good amount and is environmentally acceptable as well as safe for food production.
Assessment of Antioxidant Capacity and Putative Healthy Effects of Natural Plant Products Using Soybean Lipoxygenase-Based Methods. An Overview
Authors: Soccio Mario , Laus Maura , Flagella Zina , Pastore Donato
Published: December 2018

In the last decades, increasing demand of antioxidant-rich foods and growing interest in their putative role in prevention of degenerative diseases have promoted development of methods for measuring Antioxidant Capacity (AC). Nevertheless, most of these assays use radicals and experimental conditions far from the physiological ones, and are able to estimate only one or a few antioxidant mechanisms. On the other hand, the novel LOX/RNO and LOX–FL methods, based on secondary reactions between the soybean lipoxygenase (LOX)-1 isoenzyme and either 4-nitroso-N,N-dimethylaniline (RNO) or fluorescein (FL), may provide a more comprehensive AC evaluation. In fact, they are able to detect simultaneously many antioxidant functions (scavenging of some physiological radical species, iron ion reducing and chelating activities, inhibition of the pro-oxidant apoenzyme) and to highlight synergism among phytochemicals. They are applied to dissect antioxidant properties of several natural plant products: food-grade antioxidants, cereal and pseudocereal grains, grain-derived products, fruits. Recently, LOX–FL has been used for ex vivo AC measurements of human blood samples after short- and long-term intakes of some of these foods, and the effectiveness in improving serum antioxidant status was evaluated using the novel Antioxidant/Oxidant Balance (AOB) parameter, calculated as an AC/Peroxide Level ratio. An overview of data is presented.
Determination of Milk Products in Ceramic Vessels of Corded Ware Culture from a Late Eneolithic Burial
Authors: Kučera Lukáš , Peška Jaroslav , Fojtík Pavel , Barták Petr , Sokolovská Diana , Pavelka Jaroslav , Komárková Veronika , Beneš Jaromír , Polcerová Lenka , Králík Miroslav , Bednář Petr
Published: December 2018

In this study, a soil from two ceramic vessels belonging to Corded Ware culture, 2707–2571 B.C., found in a cremation grave discovered in Central Moravia, Czech Republic, was analyzed using matrix-assisted laser desorption/ionization–mass spectrometry (MALDI–MS) combined with advanced statistical treatment (principal component analysis, PCA, and orthogonal projection to latent structures discriminant analysis, OPLS-DA) and by enzyme-linked immunosorbent assay (ELISA). MALDI–MS revealed the presence of triacylglycerols in both vessels. This analytical technique was used for the analysis of the soil content from archaeological ceramic vessels for the first time. Targeted ELISA experiments consequently proved the presence of milk proteins in both ceramic vessels. These results represent the first direct evidence of the use of milk or dairy products in the Eneolithic period in Moravian Corded Ware Culture and help to better understand the diet habits and living conditions of Eneolithic populations in Central Europe.
Dietary Lignans: Definition, Description and Research Trends in Databases Development
Authors: Durazzo Alessandra , Lucarini Massimo , Camilli Emanuela , Marconi Stefania , Gabrielli Paolo , Lisciani Silvia , Gambelli Loretta , Aguzzi Altero , Novellino Ettore , Santini Antonello , Turrini Aida , Marletta Luisa
Published: December 2018

The study aims to communicate the current status regarding the development and management of the databases on dietary lignans; within the phytochemicals, the class of the lignan compounds is of increasing interest because of their potential beneficial properties, i.e., anticancerogenic, antioxidant, estrogenic, and antiestrogenic activities. Furthermore, an introductory overview of the main characteristics of the lignans is described here. In addition to the importance of the general databases, the role and function of a food composition database is explained. The occurrence of lignans in food groups is described; the initial construction of the first lignan databases and their inclusion in harmonized databases at national and/or European level is presented. In this context, some examples of utilization of specific databases to evaluate the intake of lignans are reported and described.
Effects of Toona sinensis Leaf Extract and Its Chemical Constituents on Xanthine Oxidase Activity and Serum Uric Acid Levels in Potassium Oxonate-Induced Hyperuricemic Rats
Authors: Yuk Heung Joo , Lee Young-Sil , Ryu Hyung Won , Kim Seung-Hyung , Kim Dong-Seon
Published: December 2018

Toona sinensis leaf is used as a seasonal vegetable in Korea. A 70% ethanol extract of these leaves exhibited potent xanthine oxidase (XO) inhibition, with a 50% inhibitory concentration (IC50) of 78.4 µM. To investigate the compounds responsible for this effect, bioassay-guided purification led to the isolation of five constituents, identified as quercetin-3-O-rutinoside, quercetin-3-O-β-d-glucopyranoside, 1,2,3,4,6-penta-O-galloyl-β-d-glucopyranose (compound 3), quercetin-3-O-α-l-rhamnopyranoside, and kaempferol-3-O-α-l-rhamnopyranoside. Compound 3 showed the most potent inhibition of XO, with an IC50 of 2.8 µM. This was similar to that of allopurinol (IC50 = 2.3 µM), which is used clinically to treat hyperuricemia. Kinetic analyses found that compound 3 was a reversible noncompetitive XO inhibitor. In vivo, the T. sinensis leaf extract (300 mg/kg), or compound 3 (40 mg/kg), significantly decreased serum uric acid levels in rats with potassium oxonate-induced hyperuricemia. Furthermore, ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry analysis identified a high level of compound 3 in the leaf extract. These findings suggest that T. sinensis leaves could be developed to produce nutraceutical preparations.
Spectrum-Effect Relationships between High-Performance Liquid Chromatography (HPLC) Fingerprints and the Antioxidant and Anti-Inflammatory Activities of Collagen Peptides
Authors: Wang Junwen , Luo Dan , Liang Ming , Zhang Ting , Yin Xiquan , Zhang Ying , Yang Xiangliang , Liu Wei
Published: December 2018

A total of 13 batches of collagen peptide samples were extracted, isolated, and purified from chicken sternal cartilage under various process parameters. The fingerprint profiles of 13 batches of collagen peptides were established by high-performance liquid chromatography (HPLC). In addition, the amino acid profiles and molecular weight distributions of collagen peptides were investigated. The in vitro antioxidant activities of the peptide samples were measured using the 2,2′-Azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) assay, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, the ferric-reducing antioxidant power (FRAP) assay and an assay of the oxidative damage induced by hydrogen peroxide (H2O2) in the degenerative cartilage cells from the knee joint of rat C518 (C518 cell line). The anti-inflammatory activities of the peptide samples were assessed by measuring the inflammatory responses induced by lipopolysaccharides (LPS) in C518 cells. Subsequently, the spectrum-effect relationships between HPLC fingerprints and the antioxidant and anti-inflammatory activities of collagen peptides were investigated using grey relational analysis (GRA). Fifteen common peaks were obtained from the HPLC fingerprints of collagen peptides. Each collagen peptide sample had a characteristic set of amino acid types and contents. All of the hydrolysates of the collagen peptides were primarily composed of fractions II (500–1000 Da) and III (1000–3000 Da). Collagen peptides exhibited good scavenging activity on ABTS radical, DPPH radical, and ferric-reducing antioxidant power. Collagen peptides were also effective against H2O2-induced cellular oxidative damage in C518 cells. The antioxidant activity of collagen peptides was due to the low molecular weight and the presence of antioxidant and hydrophobic amino acid residues within its sequence. Collagen peptides significantly inhibited the secretion of inflammatory cytokines IL-1β, TNF-α, and PGE2 in C518 cells. The anti-inflammatory activity of collagen peptides may include increased synthesis of the key components of extracellular matrix (ECM) and inhibited apoptosis of chondrocytes. The GRA results showed that peaks 2, 3, and 8 were the main components contributing to the antioxidant activity of the collagen peptides, whereas peaks 11 and 14 were the main components contributing to the anti-inflammatory activity of the collagen peptides. The components of peaks 8 and 14 were identified as GPRGPPGPVGP and VAIQAVLSLYASGR by UPLC-MS/MS. Those identified collagen peptides offer a potential therapeutic strategy for the treatment of osteoarthritis (OA) due to their antioxidative stress and due to them disturbing the catabolism and anabolism processes in arthrodial cartilage.
Vibrational Spectroscopy for Cocrystals Screening. A Comparative Study
Authors: Rodrigues Marisa , Lopes João , Sarraguça Mafalda
Published: December 2018

A recurrent problem faced by the pharmaceutical industry when formulating drug products concerns poorly soluble drugs, which, despite having desirable pharmacological activity, present limited bioavailability. Cocrystallization is growing up as a possible approach to tackle this problem. Cocrystals are crystalline materials comprising at least two components, solid at room temperature, and held together by non-covalent bonds. The increasing interest in these compounds is steadily demanding faster, simpler, and more reliable methods for the task of screening new cocrystals. This work aims at comparing the performance of three vibrational spectroscopy techniques (mid infrared, near infrared, and Raman spectroscopy) for cocrystals screening. Presented results are based on hydrochlorothiazide, a poorly soluble drug belonging to class IV of the Biopharmaceutical Classification System. The implemented cocrystal screening procedure tested six coformers (all considered safe for human administration) added according to a drug:coformer ratio of 1:1 and 1:2 and seven solvents with different polarity. The screening method chosen was based on slurry cocrystallization performed by sonication (ultrasound assisted) in a 96-well plate. Results show that all evaluated vibrational spectroscopy techniques provided important information regarding cocrystal formation, including information on the groups involved in the cocrystallization and purity, and can be used for the screening task.
The High Density Polyethylene Composite with Recycled Radiation Cross-Linked Filler of rHDPEx
Authors: Manas David , Manas Miroslav , Mizera Ales , Stoklasek Pavel , Navratil Jan , Sehnalek Stanislav , Drabek Pavel
Published: December 2018

This article discusses the possibilities of using radiation cross-linked high density polyethylene (HDPEx) acting as a filler in the original high density polyethylene (HDPE) matrix. The newly created composite is one of the possible answers to questions relating to the processing of radiation cross-linked thermoplastics. Radiation cross-linked networking is—nowadays, a commonly used technology that can significantly modify the properties of many types of thermoplastics. This paper describes the influence of the concentration of filler, in the form of grit or powder obtained by the grinding/milling of products/industrial waste from radiation cross-linked high density polyethylene (rHDPEx) on the mechanical and processing properties and the composite structure. It was determined that, by varying the concentration of the filler, it is possible to influence the mechanical behaviour of the composite. The mechanical properties of the new composite—measured at room temperature, are generally comparable or better than the same properties of the original thermoplastic. This creates very good assumptions for the effective and economically acceptable, processing of high density polyethylene (rHDPEx) waste. Its processability however, is limited; it can be processed by injection moulding up to 60 wt %.
Thermoplastic Processing of PLA/Cellulose Nanomaterials Composites
Authors: Mokhena T. C. , Sefadi J. S. , Sadiku E. R. , John M. J. , Mochane M. J. , Mtibe A.
Published: December 2018

Over the past decades, research has escalated on the use of polylactic acid (PLA) as a replacement for petroleum-based polymers. This is due to its valuable properties, such as renewability, biodegradability, biocompatibility and good thermomechanical properties. Despite possessing good mechanical properties comparable to conventional petroleum-based polymers, PLA suffers from some shortcomings such as low thermal resistance, heat distortion temperature and rate of crystallization, thus different fillers have been used to overcome these limitations. In the framework of environmentally friendly processes and products, there has been growing interest on the use of cellulose nanomaterials viz. cellulose nanocrystals (CNC) and nanofibers (CNF) as natural fillers for PLA towards advanced applications other than short-term packaging and biomedical. Cellulosic nanomaterials are renewable in nature, biodegradable, eco-friendly and they possess high strength and stiffness. In the case of eco-friendly processes, various conventional processing techniques, such as melt extrusion, melt-spinning, and compression molding, have been used to produce PLA composites. This review addresses the critical factors in the manufacturing of PLA-cellulosic nanomaterials by using conventional techniques and recent advances needed to promote and improve the dispersion of the cellulosic nanomaterials. Different aspects, including morphology, mechanical behavior and thermal properties, as well as comparisons of CNC- and CNF-reinforced PLA, are also discussed.
Feminisms and the Study of Religion in the 21st Century
Authors: Juschka Darlene M.
Published: December 2018

The articles included in this special issue on feminisms and the study of systems of belief and practice are arranged in three large sections that speak to the general orientation of the papers within the section. [...]
The Constituent Year Effect: Relative Age Disparities in Australian Masters Track and Field Athletic Participation
Authors: Medic Nikola , Lares Jasmine , Young Bradley W.
Published: December 2018

The constituent year effect, a source of relative age disparities, in masters sport has been demonstrated mainly amongst North American samples. Thus, the purpose of this study was to examine whether a participation-related constituent year effect exists among athletes (n = 6492) competing in Australian Masters Athletics competitions between 2000 and 2014. The results indicated that a participation-related constituent year effect was observed as the likelihood of participating was significantly higher for masters athletes in their first and second constituent year of any five-year age category (p < 0.0001) and was lower when they were in the fourth or fifth constituent year. The results also indicated this effect is influenced by gender and age. Specifically, the effect was significant for both male (p < 0.0001) and female (p < 0.001) masters athletes; as well during the third, sixth, seventh, and eighth + decades of life (all ps < 0.001). These data demonstrate that despite masters sport being an avenue for promotion of participation and overall health, there is potential for improving how competitive organizational strategies are implemented given the recurring intermittent patterns of participation associated with five-year age brackets which are likely to compromise benefits.
Geochemical Evolution of Fluoride and Implication for F− Enrichment in Groundwater: Example from the Bilate River Basin of Southern Main Ethiopian Rift
Authors: Haji Muhammed , Wang Dongdong , Li Lu , Qin Dajun , Guo Yi
Published: December 2018

Groundwater is the most important source of drinking water. Fluoride was found in high concentrations in the groundwater from deep wells of the water supply in the southern main Ethiopian rift. The high concentration of fluoride is dominantly geogenic rather than anthropogenic in origin, as the agricultural area was not found to be contaminated with NO3−. Knowledge of fluoride enrichment will help to provide management plans for developing deep groundwater and minimizing the health risks of exposure to fluoride. The chemical processes of fluoride were investigated in the waters in the Bilate River basin using hydrochemical and isotopic tools. The F− concentration ranged from 0.5 to 1.29 mg/L in water from shallow wells and from 0.48 to 5.61 mg/L in water from deep wells. Seventy percent of deep well samples had F− > 1.5 mg/L higher than the World Health Organization potable guideline. The high fluoride concentration in the groundwater was mainly situated in the rift valley of the Bilate River basin, in contrast with low F− groundwater in the highland. The concentration of fluoride was lowest in Ca-Mg-HCO3 type groundwater and highest in Na-HCO3 type groundwater. Moreover, F− was positively correlated with HCO3−, Na+, Na+/Ca2+ and pH in groundwater and Na+/Ca2+ ratios were increased along the flow path. Hydrogeological, hydrodynamic and hydrochemical conditions are responsible for fluoride accumulation in the deep aquifers. Strong dynamic flow in highland areas flush away weathered chemical components (e.g., F−). Thus, surficial weathering is not a major controlling factor for high concentrations of Fluoride in deep groundwater but the combination of silicate hydrolysis and ion exchange mainly control fluoride enrichment in stagnant flow environments.
Using Adjacent Buoy Information to Predict Wave Heights of Typhoons Offshore of Northeastern Taiwan
Authors: Wei Chih-Chiang , Hsieh Chia-Jung
Published: December 2018

In the northeastern sea area of Taiwan, typhoon-induced long waves often cause rogue waves that endanger human lives. Therefore, having the ability to predict wave height during the typhoon period is critical. The Central Weather Bureau maintains the Longdong and Guishandao buoys in the northeastern sea area of Taiwan to conduct long-term monitoring and collect oceanographic data. However, records have often become lost and the buoys have suffered other malfunctions, causing a lack of complete information concerning wind-generated waves. The goal of the present study was to determine the feasibility of using information collected from the adjacent buoy to predict waves. In addition, the effects of various factors such as the path of a typhoon on the prediction accuracy of data from both buoys are discussed herein. This study established a prediction model, and two scenarios were used to assess the performance: Scenario 1 included information from the adjacent buoy and Scenario 2 did not. An artificial neural network was used to establish the wave height prediction model. The research results demonstrated that (1) Scenario 1 achieved superior performance with respect to absolute errors, relative errors, and efficiency coefficient (CE) compared with Scenario 2; (2) the CE of Longdong (0.802) was higher than that of Guishandao (0.565); and (3) various types of typhoon paths were observed by examining each typhoon. The present study successfully determined the feasibility of using information from the adjacent buoy to predict waves. In addition, the effects of various factors such as the path of a typhoon on the prediction accuracy of both buoys were also discussed.