bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Decomposition and Combination Technique for Forecasting Monthly Electricity Consumption
Authors: Zhang Xi , Li Rui
Published: December 2021

With the share of electricity in total final energy consumption increasing quickly, the world is becoming increasingly dependent on electricity, which makes it more and more important to improve the forecasting accuracy of electricity consumption to ensure the normal operation of economic activities. In this paper, a novel decomposition and combination technique to forecast monthly electricity consumption is proposed. First, we use STL decomposition to obtain the trend, season, and residual components of the time series. Second, we use SARIMA, SVR, ANN, and LSTM to forecast trend, season, and residual component, respectively. Third, we use time correlation principle to improve the forecasting accuracy of season component. Fourth, we integrated the residual component predicted by SARIMA, SVR, ANN, and LSTM into a new sequence to improve the forecasting accuracy of residual component. In order to verify the performance of the proposed forecast model, monthly electricity consumption data in China is introduced as an example for empirical analysis. The results show that after STL decomposition, time correlation modification, and residual modification, the forecasting accuracy of each model has been gradually improved. We believe that the proposed forecast model in this paper can also be used to solve other mid- and long-term forecasting problems with obvious seasonal characteristics.
Facile Construction of Hierarchical TiNb2O7/rGO Nanoflower With Robust Charge Storage Properties for Li Ion Batteries via an Esterification Reaction
Authors: Hu Lei , Yang Xulai , Chen Yumeng , Wang Lili , Li Jiajia , Tang Yujie , Zhang Haitao
Published: December 2021

TiNb2O7 (TNO) compound has been pursued tremendously due to its high theoretical capacity, high potential, and excellent cycle stability. Unfortunately, an intrinsic low electronic and ionic conductivity feature has restricted its broad applications in electrochemical energy storage fields. Two-dimensional (2D) nanostructures can effectively shorten Li-ion transport path and enhance charge transfer. Here, hierarchical structure TNO was constructed by using ethanol and acetic acid as particularly important organic chemicals of basic raw materials via a simple solvothermal reaction. Ethanol was found to play a critical role in the formation of 2D sheet structure. Meantime, reduced graphene oxide nanosheets can effectively improve electronic conductivity. As-obtained TiNb2O7 were wrapped further by graphene oxide nanosheets through a flocculation process. Their unique structure is beneficial to the final electrochemical performance. This study not only provides a general approach for the design of novel 2D nanomaterials wrapped by graphene because of the advantage of esterification reaction and flocculation reaction, but also improves the electronic and ionic conductivity simultaneously.
Does Globalization Moderate the Effect of Economic Complexity on CO2 Emissions? Evidence From the Top 10 Energy Transition Economies
Authors: He Kai , Ramzan Muhammad , Awosusi Abraham Ayobamiji , Ahmed Zahoor , Ahmad Mahmood , Altuntaş Mehmet
Published: December 2021

The association between economic complexity (sophisticated economic structure) and carbon emissions has major implications for environmental sustainability. In addition, globalization can be an important tool for attaining environmental sustainability and it may also moderate the association between economic complexity and carbon emissions. Thus, this research examines the effects of economic complexity, economic growth, renewable energy, and globalization on CO2 emissions in the top 10 energy transition economies where renewable energy and globalization have greatly increased over the last 3 decades. Furthermore, this study also evaluates the joint effect of globalization and economic complexity on carbon emissions. Keeping in view the presence of slope heterogeneity and cross-sectional dependence in the data, this research utilized second-generation unit root tests (CIPS and CADF), Westerlund cointegration approach, and CS-ARDL and CCEMG long-run estimators over the period of 1990–2018. The results affirmed the presence of cointegration among the considered variable. Long-run findings revealed that globalization, renewable energy, and economic complexity decrease carbon emissions. Conversely, economic growth increases carbon emissions. Moreover, the joint impact of economic complexity and globalization stimulates environmental sustainability. Based on these findings, the government of these groups of economies should continue to expand the usage of renewable energy. They should also promote interaction with the rest of the world by adopting the policy of opening up.
Microplastics Pollution in Chile: Current Situation and Future Prospects
Authors: Paredes-Osses Esteban , Pozo Karla , Opazo-Capurro Andrés , Bahamonde Paulina , Cabrera-Pardo Jaime R.
Published: December 2021

Millions of tons of plastics enter wild habitats, especially the oceans, every year. Despite extensive efforts, this amount is predicted to increase over in the near future, leading to a catastrophic damage to the environment. Small plastic fragments, including microplastics, are currently widely distributed in different environments and contribute significantly to pollution of the oceans. This problem is particularly poignant in Chile, a country with more than 4,000 km of coastline along the Pacific Ocean home to diverse environments, industrial activities and unique biodiversity. In this review, we compile information regarding microplastics pollution in Chilean environments in terms of transport, distribution and bioaccumulation along the country, societal actions such as environmental policies and education to tackle the plastic problem, and the Trojan effect associated with it. Finally, we identify critical scientific gaps, such as the transport of harmful chemicals and microbial communities associated, and define potential future research directions.
Using Model Analysis to Unveil Hidden Patterns in Tropical Forest Structures
Authors: Picard Nicolas , Mortier Frédéric , Ploton Pierre , Liang Jingjing , Derroire Géraldine , Bastin Jean-François , Ayyappan Narayanan , Bénédet Fabrice , Boyemba Bosela Faustin , Clark Connie J. , Crowther Thomas W. , Engone Obiang Nestor Laurier , Forni Éric , Harris David , Ngomanda Alfred , Poulsen John R. , Sonké Bonaventure , Couteron Pierre , Gourlet-Fleury Sylvie
Published: December 2021

When ordinating plots of tropical rain forests using stand-level structural attributes such as biomass, basal area and the number of trees in different size classes, two patterns often emerge: a gradient from poorly to highly stocked plots and high positive correlations between biomass, basal area and the number of large trees. These patterns are inherited from the demographics (growth, mortality and recruitment) and size allometry of trees and tend to obscure other patterns, such as site differences among plots, that would be more informative for inferring ecological processes. Using data from 133 rain forest plots at nine sites for which site differences are known, we aimed to filter out these patterns in forest structural attributes to unveil a hidden pattern. Using a null model framework, we generated the anticipated pattern inherited from individual allometric patterns. We then evaluated deviations between the data (observations) and predictions of the null model. Ordination of the deviations revealed site differences that were not evident in the ordination of observations. These sites differences could be related to different histories of large-scale forest disturbance. By filtering out patterns inherited from individuals, our model analysis provides more information on ecological processes.
Cooperative Behaviors in Group-Living Spider Mites
Authors: Schausberger Peter , Yano Shuichi , Sato Yukie
Published: December 2021

Cooperative behaviors are evolutionary stable if the direct and/or indirect fitness benefits exceed the costs of helping. Here we discuss cooperation and behaviors akin to cooperation in subsocial group-living species of two genera of herbivorous spider mites (Tetranychidae), i.e., the largely polyphagous Tetranychus spp. and the nest-building Stigmaeopsis spp., which are specialized on grasses, such as bamboo. These spider mites are distributed in patches on various spatial scales, that is, within and among leaves of individual host plants and among individual hosts of single or multiple plant species. Group-living of spider mites is brought about by plant-colonizing foundresses ovipositing at local feeding sites and natal site fidelity, and by multiple individuals aggregating in the same site in response to direct and/or indirect cues, many of which are associated with webbing. In the case of the former, emerging patches are often composed of genetically closely related individuals, while in the case of the latter, local patches may consist of kin of various degrees and/or non-kin and even heterospecific spider mites. We describe and discuss ultimate and proximate aspects of cooperation by spider mites in host plant colonization and exploitation, dispersal, anti-predator behavior, and nesting-associated behaviors and conclude with theoretical and practical considerations of future research on cooperation in these highly rewarding model animals.
Environmental Heterogeneity Determines Diatom Colonisation on Artificial Substrata: Implications for Biomonitoring in Coastal Marine Waters
Authors: Cotiyane-Pondo Phumlile , Bornman Thomas G.
Published: December 2021

Benthic diatoms form an important component of the microphytobenthos and have long been utilised as suitable bioindicators in aquatic systems. However, knowledge on benthic diatom community succession on hard substrata (biofilm) remains understudied in austral marine coastal systems. In this study, we investigated benthic diatom colonisation on artificial substrates (Plexiglass) over a period of 5 weeks at two locations with different physical environments along the warm temperate coast of South Africa. Results revealed relatively similar physico-chemical conditions but highly contrasting diatom community development were observed between the two sites. While there were some shared taxa, site-specific dynamics resulted in significantly different diatom species diversity and richness, facilitated by common (e.g.,Nitzschia ventricosaandCocconeis scutellum) and a large percentage of rarely observed species such asCocconeis testudoandLyrella lyra. A total of 134 species belonging to 44 genera were observed during the study. The overall diatom composition differed spatio-temporally during the experimental period, with the fluctuating species occurrences and abundances highlighting the rapid microalgal species turnover within days, under natural conditions. Environmental variables were shown to have varying influences as drivers of the diatom community descriptors. Multivariate modelling confirmed that study site and the interaction between site and sampling occasion were important predictors of diatom abundances, and the overall observed community composition. The current results suggest that benthic diatoms on artificial substrata could be incorporated as suitable indicators of change along the coastline subject to further investigations, taking into account site-specific differences driven by habitat complexity and environmental variability. The experimental method proved to be efficient and can be implemented to study the response of benthic diatoms to localised nutrient enrichment around the coastline.
The Dimensions and Units of the Population Interaction Coefficients
Authors: Arditi Roger , Tyutyunov Yuri V. , Titova Lyudmila I. , Rohr Rudolf P. , Bersier Louis-Félix
Published: December 2021

We discuss the interpretation and dimensions of the population dynamic parameters that are commonly used to quantify the strength of intraspecific and interspecific interactions. The concept of “interaction strength” is not unequivocal. Its theoretical formalization relies on the generalized Lotka–Volterra model. However, four different ways of parameterizing the model have been proposed in the literature, leading to four different definitions of the term “interaction strength.” In particular, the dimensions of these four definitions are not identical, some of these incorporating explicitly the dimension used to measure the population size. Using an individual-based simulation model as an illustration, we show that, in the latter case, the interaction strength depends implicitly on the habitat size. As a consequence, it is of crucial importance to quantify the population as a density rather than an absolute population abundance. We insist that the dimension of the interaction strength coefficient should not be overlooked and any quantitative estimation must be given with explicit units.
Tropical Dry Forest Resilience to Fire Depends on Fire Frequency and Climate
Authors: Hartung Maximilian , Carreño-Rocabado Geovana , Peña-Claros Marielos , van der Sande Masha T.
Published: December 2021

Wildfires are becoming increasingly frequent and devastating in many tropical forests. Although seasonally dry tropical forests (SDTF) are among the most fire-threatened ecosystems, their long-term response to frequent wildfires remains largely unknown. This study is among the first to investigate the resilience in response to fire of the Chiquitano SDTF in Bolivia, a large ecoregion that has seen an unprecedented increase in fire intensity and frequency in recent years. We used remote sensing data to assess at a large regional and temporal scale (two decades) how fire frequency and environmental factors determine the resilience of the vegetation to fire disturbance. Resilience was measured as the resistance to fire damage and post-fire recovery. Both parameters were monitored for forested areas that burned once (F1), twice (F2), and three times (F3) between 2000 and 2010 and compared to unburned forests. Resistance and recovery were analyzed using time series of the Normalized Burn Ratio (NBR) index derived from Landsat satellite imagery, and climatic, topographic, and a human development-related variable used to evaluate their influence on resilience. The overall resilience was lowest in forests that burned twice and was higher in forests that burned three times, indicating a possible transition state in fire resilience, probably because forests become increasingly adapted during recurrent fires. Climatic variables, particularly rainfall, were most influential in determining resilience. Our results indicate that the Chiquitano dry forest is relatively resilient to recurring fires, has the capacity to recover and adapt, and that climatic differences are the main determinants of the spatial variation observed in resilience. Nevertheless, further research is needed to understand the effect of the higher frequency and intensity of fires expected in the future due to climate change and land use change, which may pose a greater threat to forest resilience.
Trichoderma and Nanotechnology in Sustainable Agriculture: A Review
Authors: Ramírez-Valdespino Claudia A. , Orrantia-Borunda Erasmo
Published: December 2021

Due to their unique properties and functionalities, nanomaterials can be found in different activities as pharmaceutics, cosmetics, medicine, and agriculture, among others. Nowadays, formulations with nano compounds exist to reduce the application of conventional pesticides and fertilizers. Among the most used are nanoparticles (NPs) of copper, zinc, or silver, which are known because of their cytotoxicity, and their accumulation can change the dynamic of microbes present in the soil. In agriculture, Trichoderma is widely utilized as a safe biocontrol strategy and to promote plant yield, making it susceptible to be in contact with nanomaterials that can interfere with its viability as well as its biocontrol and plant growth promotion effects. It is well-known that strains of Trichoderma can tolerate and uptake heavy metals in their bulk form, but it is poorly understood whether the same occurs with nanomaterials. Interestingly, Trichoderma can synthesize NPs that exhibit antimicrobial activities against various organisms of interest, including plant pathogens. In this study, we summarize the main findings regarding Trichoderma and nanotechnology, including its use to synthesize NPs and the consequence that these compounds might have in this fungus and its associations. Moreover, based on these findings we discuss whether it is feasible to develop agrochemicals that combine NPs and Trichoderma strains to generate more sustainable products or not.
Simple Matching Using QIIME 2 and RDP Reveals Misidentified Sequences and an Underrepresentation of Fungi in Reference Datasets
Authors: Eldred Lauren E. , Thorn R. Greg , Smith David Roy
Published: December 2021

Simple nucleotide matching identification methods are not as accurate as once thought at identifying environmental fungal sequences. This is largely because of incorrect naming and the underrepresentation of various fungal groups in reference datasets. Here, we explore these issues by examining an environmental metabarcoding dataset of partial large subunit rRNA sequences of Basidiomycota and basal fungi. We employed the simple matching method using the QIIME 2 classifier and the RDP Classifier in conjunction with the latest releases of the SILVA (138.1, 2020) and RDP (11, 2014) reference datasets and then compared the results with a manual phylogenetic binning approach. Of the 71 query sequences tested, 21 and 42% were misidentified using QIIME 2 and the RDP Classifier, respectively. Of these simple matching misidentifications, more than half resulted from the underrepresentation of various groups of fungi in the SILVA and RDP reference datasets. More comprehensive reference datasets with fewer misidentified sequences will increase the accuracy of simple matching identifications. However, we argue that the phylogenetic binning approach is a better alternative to simple matching since, in addition to better accuracy, it provides evolutionary information about query sequences.
Identifying a Hypoxia-Related Long Non-Coding RNAs Signature to Improve the Prediction of Prognosis and Immunotherapy Response in Hepatocellular Carcinoma
Authors: Tang Pingfei , Qu Weiming , Wang Taoli , Liu Minji , Wu Dajun , Tan Lin , Zhou Hongbing
Published: December 2021

Abstract Background: Both hypoxia and long non-coding RNAs (lncRNAs) contribute to the tumor progression in hepatocellular carcinoma (HCC). We sought to establish a hypoxia-related lncRNA signature and explore its correlation with immunotherapy response in HCC.

Materials and Methods: Hypoxia-related differentially expressed lncRNAs (HRDELs) were identified by conducting the differential gene expression analyses in GSE155505 and The Cancer Genome Atlas (TCGA)- liver hepatocellular carcinoma (LIHC) datasets. The HRDELs landscape in patients with HCC in TCGA-LIHC was dissected by an unsupervised clustering method. Patients in the TCGA-LIHC cohort were stochastically split into the training and testing dataset. The prognostic signature was developed using LASSO (least absolute shrinkage and selection operator) penalty Cox and multivariable Cox analyses. The tumor immune microenvironment was delineated by the single-sample gene set enrichment analysis (ssGSEA) algorithm. The Tumor Immune Dysfunction and Exclusion (TIDE) algorithm was applied to evaluate the predictive value of the constructed signature in immunotherapeutic responsiveness.

Results: A total of 55 HRDELs were identified through integrated bioinformatical analyses in GSE155505 and TCGA-LIHC. Patients in the TCGA-LIHC cohort were categorized into three HRDELs-specific clusters associated with different clinical outcomes. The prognostic signature involving five hypoxia-related lncRNAs (LINC00869, CAHM, RHPN1-AS1, MKLN1-AS, and DUXAP8) was constructed in the training dataset and then validated in the testing dataset and entire TCGA-LIHC cohort. The 5-years AUC of the constructed signature for prognostic prediction reaches 0.705 and is superior to that of age, AJCC stage, and histopathological grade. Patients with high-risk scores consistently had poorer overall survival outcomes than those with low-risk scores irrespective of other clinical parameters status. The low-risk group had more abundance in activated CD8+ T cell and activated B cell and were predicted to be more responsive to immunotherapy and targeted therapy than the high-risk group.

Conclusion: We established a reliable hypoxia-related lncRNAs signature that could accurately predict the clinical outcomes of HCC patients and correlate with immunotherapy response and targeted drug sensitivity, providing new insights for immunotherapy and targeted therapy in HCC.
The Prolactin Family of Hormones as Regulators of Maternal Mood and Behavior
Authors: Georgescu Teodora , Swart Judith M. , Grattan David R. , Brown Rosemary S. E.
Published: December 2021

Transition into motherhood involves profound physiological and behavioral adaptations that ensure the healthy development of offspring while maintaining maternal health. Dynamic fluctuations in key hormones during pregnancy and lactation induce these maternal adaptations by acting on neural circuits in the brain. Amongst these hormonal changes, lactogenic hormones (e.g., prolactin and its pregnancy-specific homolog, placental lactogen) are important regulators of these processes, and their receptors are located in key brain regions controlling emotional behaviors and maternal responses. With pregnancy and lactation also being associated with a marked elevation in the risk of developing mood disorders, it is important to understand how hormones are normally regulating mood and behavior during this time. It seems likely that pathological changes in mood could result from aberrant expression of these hormone-induced behavioral responses. Maternal mental health problems during pregnancy and the postpartum period represent a major barrier in developing healthy mother-infant interactions which are crucial for the child's development. In this review, we will examine the role lactogenic hormones play in driving a range of specific maternal behaviors, including motivation, protectiveness, and mother-pup interactions. Understanding how these hormones collectively act in a mother's brain to promote nurturing behaviors toward offspring will ultimately assist in treatment development and contribute to safeguarding a successful pregnancy.
Examining the Associations Between Online Interactions and Momentary Affect in Depressed Adolescents
Authors: Moukalled Summer H. , Bickham David S. , Rich Michael
Published: December 2021

Concern has been raised over parallel increases in youth depression and online interactive media use over the past two decades. The aim of this study was to determine whether online interactions are associated with users’ affective states. Using ecological momentary assessment, we measured depressed adolescents’ momentary affect during and residual feelings following online interactions with offline friends and family, online friends, and acquaintances/strangers. We found that depressed adolescents use texting services and social networking sites to interact online, most frequently with offline friends and family, followed by online friends. Results of generalized estimating equations showed associations between negative affect and digital interactions with offline friends and family. Participants were less likely to report feeling better after interacting with online friends than after interacting with any other relationship type. Our findings highlight the heterogeneity of depressed adolescents’ online interactions and suggest that their affective experience varies depending on the nature of the relationships they have with those with whom they interact.
Militarisation Under COVID-19: Understanding the Differential Impact of Lockdown on the Forests of Colombia
Authors: Amador-Jiménez Mónica , Millner Naomi
Published: December 2021

Drawing on qualitative analysis and anthropological histories, we argue that deforestation rates in the Inter-Andean Valleys and in the Amazon Belt of Colombia reflect the specific role of the military in different articulations of the political forest along with new connections between conservation and the war on drugs. This paper examines the increase in deforestation in Colombia in 2020 that partially coincided with the “lockdown” imposed to curb the spread of COVID-19. Early media analysis linked this with the redeployment of military forces away from forest protection to impose lockdown restrictions. However, closer investigation reveals significant regional variation in both the reorganisation of military groups, and in the rate at which deforestation has materialised; military presence has increased in some regions, while in others deforestation has increased. To explain this, we unpack the “biopolitical” dimensions of international conservation to show how the specific deployment of military groups in Colombia reflects an interplay between notions of the protection of (species) life, longer colonial histories, and more recent classification of geographies in terms of riskiness and value.
Anti-Angiogenic Agent Combined with Anti-PD-1 Immunotherapy Showed Activity in Patients With Classical Hodgkin Lymphoma Who Have Failed Immunotherapy: A Retrospective Case Report Study
Authors: Yan Zheng , Ma Jialin , Yao Shuna , Yao Zhihua , Wang Haiying , Chu Junfeng , Zhao Shuang , Liu Yanyan
Published: December 2021

Background

PD-1/PD-L1 inhibitor immunotherapy has showed impressive activity in various cancers, especially relapsed/refractory (r/r) classical Hodgkin lymphoma (cHL). However, acquired resistance is inevitable for most patients. Sometimes severe side effects also lead to treatment termination. When immunotherapy failed, alternative treatment options are limited. In the past few years, we have used the anti-angiogenic agent apatinib and PD-1 inhibitor camrelizumab to treat cHL patients who failed prior immunotherapy. In this study, we analyzed the data of these patients.

Patients and Methods

Patients with r/r cHL who had failed immunotherapy and subsequently received apatinib-camrelizumab (AC) combination therapy were included in this study. Patient data were collected from medical records and follow-up system. The efficacy and safety of AC therapy were analyzed.

Results

Seven patients who failed immunotherapy were identified in our database, of which five patients acquired immunotherapy resistance and two patients experienced severe side effects. They received a combination of camrelizumab (200 mg every four weeks) and apatinib (425 mg or 250 mg per day). As of the cut-off date, these patients had received a median of 4 cycles (range, 2 - 31) of treatment. Two (2/7) patients achieved complete response, four (4/7) partial response, and one (1/7) stable disease. The median progression-free survival was 10.0 months (range, 2.0 – 27.8). Low-dose apatinib (250 mg) plus camrelizumab was well tolerated and had no unexpected side effects. Besides, no reactive cutaneous capillary endothelial proliferation was observed in AC-treated patients.

Conclusions

Low dose apatinib plus camrelizumab might be a promising treatment option for r/r cHL patients who have failed immunotherapy. This combination treatment is worthy of further investigation in more patients including solid cancer patients who have failed immunotherapy.
MIR337-3p Enhances Mycobacterial Pathogenicity Involving TLR4/MYD88 and STAT3 Signals, Impairing VDR Antimicrobial Response and Fast-Acting Immunity
Authors: Liang Shanshan , Huang Guixian , Wu Tian , Peng Ying , Liu Xi , Ji Xuejiao , Sha Wei , Wang Feifei , Shen Ling , Shen Hongbo
Published: December 2021

Active form of vitamin D (VitD) enhances human innate immunity against Mycobacterium tuberculosis (Mtb) infection. Our previous studies showed that MIR337-3p was highly expressed in lymphocytes of tuberculosis (TB) patients. Here, we identified the mechanism of MIR337-3p in the regulation of fast-acting anti-TB immunity by inhibiting VitD-dependent antimicrobial response pathways. While high-level MIR337-3p expression was induced by mycobacterial infection in cellular models and mice, TB patients exhibited significantly increased MIR337-3p in CD14+ monocytes/macrophages, innate-like Vγ2+ T cells, and CD8+ lymphocytes containing natural killer (NK)/innate lymphoid cells. MIR337-3p promoted the mycobacterial entry/infection and replication/growth in host target cells: macrophages and lung epithelial cells. Such MIR337-3p-enhanced pathogenicity coincided with the MIR337-3p depression of VitD-dependent antimicrobial response of cytochrome P450, family 27, subfamily b, polypeptide 1 (CYP27B1)/Beta-defensin 4 (DEFB4A)/ cathelicidin antimicrobial peptide CAMP pathways. Surprisingly, single MIR337-3p species could specifically target both the Toll-like receptor 4 (TLR4) and signal transducer and activator of transcription 3 (STAT3) 3′-untranslated regions (UTRs) to depress the TLR4/MYD88 and STAT3 signals and impair either of the two signals inhibiting the VitD-dependent antimicrobial pathways in macrophages. Concurrently, human peripheral blood mononuclear cells (PBMCs) expressing high-level MIR337-3p exhibited a reduced ability of innate cell populations to mount fast-acting cellular immunity against intracellular mycobacterial infection. Furthermore, a higher expression of Mir337-3p after mycobacterial infection of mice coincided with much greater colony-forming unit (CFU) counts in lungs and even the death of infected animals, whereas Mir337-3p inhibitor treatment of infected mice reduced Mir337-3p levels and reversed Mir337-3p-mediated increases in CFU counts. Thus, TB-driven single MIR337-3p species could specifically target/impair both TLR4/MYD88 and STAT3 activation signals, inhibiting VitD-dependent antimicrobial response and fast-acting anti-TB immunity, leading to enhanced pathogenicity.
Viral Induced Effects on a Vulnerable Epithelium; Lessons Learned From Paediatric Asthma and Eosinophilic Oesophagitis
Authors: Watkinson Rebecca L. , Looi Kevin , Laing Ingrid A. , Cianferoni Antonella , Kicic Anthony
Published: December 2021

The epithelium is integral to the protection of many different biological systems and for the maintenance of biochemical homeostasis. Emerging evidence suggests that particular children have epithelial vulnerabilities leading to dysregulated barrier function and integrity, that resultantly contributes to disease pathogenesis. These epithelial vulnerabilities likely developin uteroor in early life due to various genetic, epigenetic and environmental factors. Although various epithelia are uniquely structured with specific function, prevalent allergic-type epithelial diseases in children potentially have common or parallel disease processes. These include inflammation and immune response dysregulation stemming from atypical epithelial barrier function and integrity. Two diseases where aetiology and pathogenesis are potentially linked to epithelial vulnerabilities include Paediatric Asthma and Eosinophilic Oesophagitis (EoE). For example, rhinovirus C (RV-C) is a known risk factor for paediatric asthma development and is known to disrupt respiratory epithelial barrier function causing acute inflammation. In addition, EoE, a prevalent atopic condition of the oesophageal epithelium, is characterised by similar innate immune and epithelial responses to viral injury. This review examines the current literature and identifies the gaps in the field defining viral-induced effects on a vulnerable respiratory epithelium and resulting chronic inflammation, drawing from knowledge generated in acute wheezing illness, paediatric asthma and EoE. Besides highlighting the importance of epithelial structure and barrier function in allergic disease pathogenesis regardless of specific epithelial sub-types, this review focuses on the importance of examining other parallel allergic-type disease processes that may uncover commonalities driving disease pathogenesis. This in turn may be beneficial in the development of common therapeutics for current clinical management and disease prevention in the future.
Acute Imidacloprid Exposure Alters Mitochondrial Function in Bumblebee Flight Muscle and Brain
Authors: Sargent Chloe , Ebanks Brad , Hardy Ian C. W. , Davies T. G. Emyr , Chakrabarti Lisa , Stöger Reinhard
Published: December 2021

Mitochondria are intracellular organelles responsible for cellular respiration with one of their major roles in the production of energy in the form of ATP. Activities with increased energetic demand are especially dependent on efficient ATP production, hence sufficient mitochondrial function is fundamental. In bees, flight muscle and the brain have particularly high densities of mitochondria to facilitate the substantial ATP production required for flight activity and neuronal signalling. Neonicotinoids are systemic synthetic insecticides that are widely utilised against crop herbivores but have been reported to cause, by unknown mechanisms, mitochondrial dysfunction, decreasing cognitive function and flight activity among pollinating bees. Here we explore, using high-resolution respirometry, how the neonicotinoid imidacloprid may affect oxidative phosphorylation in the brain and flight muscle of the buff-tailed bumblebee, Bombus terrestris. We find that acute exposure increases routine oxygen consumption in the flight muscle of worker bees. This provides a candidate explanation for prior reports of early declines in flight activity following acute exposure. We further find that imidacloprid increases the maximum electron transport capacity in the brain, with a trend towards increased overall oxygen consumption. However, intra-individual variability is high, limiting the extent to which apparent effects of imidacloprid on brain mitochondria are shown conclusively. Overall, our results highlight the necessity to examine tissue-specific effects of imidacloprid on respiration and energy production.
Assessing Cephalopods Fisheries in the Strait of Sicily by Using Poor Data Modeling
Authors: Geraci Michele L. , Falsone Fabio , Gancitano Vita , Scannella Danilo , Fiorentino Fabio , Vitale Sergio
Published: December 2021

Cephalopods, including octopuses, squids, and cuttlefishes, are exploited by both bottom trawl and small-scale fisheries (SSF) in most of the Mediterranean areas. Bottom trawl fisheries regard cephalopods as a valuable bycatch, whereas for SSF, they are among the main target species. Cephalopods account for a relatively small proportion of the total landings in the Mediterranean. However, from an economic point of view, four cephalopods, Eledone cirrhosa, Eledone moschata, Octopus vulgaris, and Sepia officinalis, account for approximately 15% of the total landing value. Despite their economic importance, there are very few stock assessments of cephalopods in the Mediterranean because it is difficult to assess them by classical age-based methods, given their short life-cycles, and highly variable growth and recruitment. The production of E. cirrhosa, E. moschata, Illex coindettii, Loligo vulgaris, O. vulgaris, S. officinalis, and Todaropsis eblanae in the waters off the south of Sicily accounts for approximately 8% of the total Mediterranean yield of cephalopods. This study presents the first attempt to assess the state of these cephalopods in the Strait of Sicily by using surplus production models. Since species-wise landing statistics may be unreliable because of their morphological similarity, some octopuses (E. cirrhosa and E. moschata) and ommastrephid squids (I. coindetii and T. eblanae) were assessed combined. Landing data and abundance indices from trawl surveys were used to describe cephalopod stock dynamics through the Bayesian State Space Schaefer model (BSM) and Surplus Production model in Continuous Time (SPiCT) models. As survey data were not considered reliable indicators of their abundance, O. vulgaris, S. officinalis, and L. vulgaris stocks were assessed using the Catch-Maximum Sustainable Yield (CMSY) model. Overall, squid and cuttlefish stocks were observed to be in healthy conditions. However, assessments of octopus stocks indicated that their condition was critical or recovering. Here, we discuss the different stock statuses in the light of evolving fisheries and environmental factors in the area over time. Although cephalopods are not a priority in the current management system of Mediterranean fisheries, the importance of these species in the food web and their relevance for SSF underline their importance and their exploitation status should be periodically evaluated.
Modulation of Gilthead Sea Bream Gut Microbiota by a Bioactive Egg White Hydrolysate: Interactions Between Bacteria and Host Lipid Metabolism
Authors: Naya-Català Fernando , Wiggers Giulia A. , Piazzon M. Carla , López-Martínez Manuel I. , Estensoro Itziar , Calduch-Giner Josep A. , Martínez-Cuesta M. Carmen , Requena Teresa , Sitjà-Bobadilla Ariadna , Miguel Marta , Pérez-Sánchez Jaume
Published: December 2021

This study aimed to highlight the relationship between diet, animal performance and mucosal adherent gut microbiota (anterior intestine) in fish fed plant-based diets supplemented with an egg white hydrolysate (EWH) with antioxidant and anti-obesogenic activity in obese rats. The feeding trial with juveniles of gilthead sea bream (Sparus aurata) lasted 8 weeks. Fish were fed near to visual satiety with a fish meal (FM)/fish oil (FO) based diet (CTRL) or a plant-based diet with/without EWH supplementation. Specific growth rate decreased gradually from 2.16% in CTRL fish to 1.88% in EWH fish due to a reduced feed intake, and a slight impairment of feed conversion ratio. Plant-based diets feeding triggered a hyperplasic inflammation of the anterior intestine regardless of EWH supplementation. However, EWH ameliorated the goblet cell depletion, and the hepatic and intestinal lipid accumulation induced by FM/FO replacement. Illumina sequencing of gut mucosal microbiota yielded a mean of 136,252 reads per sample assigned to 2,117 OTUs at 97% identity threshold. The bacterial diversity was similar in all groups, but a significantly lower richness was found in EWH fish. At the phylum level, Proteobacteria reached the highest proportion in CTRL and EWH fish, whereas Firmicutes were decreased and Actinobacteria increased with the FM/FO replacement. The proportion of Actinobacteria was restored by dietary EWH supplementation, which also triggered a highest amount of Bacteroidetes and Spirochaetes. At a closer look, a widespread presence of Lactobacillales among groups was found. Otherwise, polysaccharide hydrolases secretors represented by Corynebacterium and Nocardioides were increased by the FM/FO replacement, whereas the mucin-degrading Streptococcus was only raised in fish fed the plant-based diet without EWH. In addition, in EWH fish, a higher abundance of Propionibacterium was related to an increased concentration of intestinal propionate. The antagonism of gut health-promoting propionate with cholesterol could explain the inferred underrepresentation of primary bile acid biosynthesis and steroid degradation pathways in the EWH fish microbiota. Altogether, these results reinforce the central role of gut microbiota in the regulation of host metabolism and lipid metabolism in particular, suggesting a role of the bioactive EWH peptides as an anti-obesity and/or satiety factor in fish.
The Diversity and Nitrogen Metabolism of Culturable Nitrate-Utilizing Bacteria Within the Oxygen Minimum Zone of the Changjiang (Yangtze River) Estuary
Authors: He Wenxuan , Liu Sizhen , Jiang Zhichen , Zheng Jinshui , Li Xuegang , Zhang Dechao
Published: December 2021

The nitrogen cycle is an indispensable part of the biogeochemical cycle, and the reactions that occur in the ocean oxygen minimum zone (OMZ) mediate much of the loss of nitrogen from oceans worldwide. Here, nitrate-utilizing bacteria were isolated from the water column at 17 stations within the OMZ of the Changjiang (Yangtze River) Estuary using selective media and a culture-dependent method. The microbial diversity, nitrogen metabolism and nitrate reduction test of culturable heterotrophic bacteria were examined. A total of 164 isolates were obtained; they were mostly affiliated with Proteobacteria (81.1%), Actinobacteria (5.5%), Bacteroidetes (12.3%), and Firmicutes (0.6%). Pseudomonas aeruginosa, Sphingobium naphthae, and Zunongwangia profunda were found at most stations. Among 24 tested representative strains, 8 were positive for nitrate reduction; they belonged to genera Aurantimonas, Halomonas, Marinobacter, Pseudomonas, Thalassospira, and Vibrio. Pseudomonas aeruginosa contained the genes (napAB, norBC, nirS, and nosZ) for complete denitrification and may be responsible for mediating denitrification. 66% representative isolates (16/24) contained genes for reducing nitrate to nitrite (nasA, napAB, or narGHI) and 79% representative isolates (19/24) possessed genes for converting nitrite to ammonia (nirA or nirBD), suggesting that nitrate and nitrite could act as electron acceptors to generate ammonium, subsequently being utilized as a reduced nitrogen source. This study improves our understanding of the microbial diversity within the OMZ of Changjiang Estuary and may facilitate the cultivation and exploitation of bacteria involved in the nitrogen cycle.
Understanding the Dynamics of Ancillary Pelagic Species in the Adriatic Sea
Authors: Angelini Silvia , Armelloni Enrico N. , Costantini Ilaria , De Felice Andrea , Isajlović Igor , Leonori Iole , Manfredi Chiara , Masnadi Francesco , Scarcella Giuseppe , Tičina Vjekoslav , Santojanni Alberto
Published: December 2021

The status of fishery resources in the Mediterranean Sea is critical: most of the fish and shellfish stocks are in overexploitation and only half of them are routinely assessed. This manuscript presents the use of Surplus Production Models (SPMs) as a valid option to increase the number of assessed stocks, with specific attention to the Adriatic basin. Particularly, the stock of European sprat (Sprattus sprattus), Mediterranean horse mackerel (Trachurus mediterraneus), and Atlantic horse mackerel (Trachurus trachurus) living in the Adriatic Sea have been evaluated comparing three SPMs: Catch Maximum Sustainable Yields (CMSY), Stochastic surplus Production model in Continuous Time (SPiCT), and Abundance Maximum Sustainable Yields (AMSY). The different approaches present some variations; however, they generally agree on describing all the stocks close to the reference values for both biomass and fishing mortality in the most recent year. For the European sprat, AMSY results are the most robust model for this species’ survey data allow depicting a clearer picture of the history of this stock. Indeed, for the horse mackerel species, CMSY or SPiCT results are the preferred models, since for these species landings are not negligible. Notwithstanding, age-structured assessments remain the most powerful approach for evaluating the status of resources, but SPMs have proved to be a powerful tool in a data-limited context.
Internal Solitary Waves Observed on the Continental Shelf in the Northern South China Sea From Acoustic Backscatter Data
Authors: Feng Yingci , Tang Qunshu , Li Jian , Sun Jie , Zhan Wenhuan
Published: December 2021

Internal solitary waves (ISWs) are investigated offshore of Guangdong in the northern South China Sea (SCS) using high-frequency acoustic backscatter data of 100 kHz acquired in July 2020. Simultaneous XBT profiles and satellite images are incorporated to understand their propagation, evolution, and dissipation processes in shallow water at depths less than 50 m. The water column structures revealed by acoustic backscatter data and XBT profiles are consistent with a small difference of less than 3 m. A soliton train with apparent vertical and horizontal scales of ∼7 and 100 m, respectively, is captured three times in 20 h in the repeated acoustic sections, which provides spatiotemporal constraints to the solitons. The characteristics of ISW phase speeds are estimated from acoustic backscatter data and satellite data and using theoretical two-layer Korteweg-de Vries (KdV) and extended KdV (eKdV) models. The acoustically observed phase speed of ISWs is approximately 0.4–0.5 m/s, in agreement with the estimates from both satellite data and model results. The shallow solar-heated water in summer (∼10–20 m) lying on the bottom cold water is responsible for the extensive occurrence of ISWs in the study region. ISWs are dissipated at the transition zone between the heated surface water and the upwelled water, forming a wide ISW dissipation zone in the coastal area, as observed from satellites. The acoustic backscatter method could be an effective way to observe ISWs with high resolution in shallow water and thus a potential compensatory technique for imaging the shallow blind zone of so-called seismic oceanography.
Photobleaching and Recovery of Symbiodiniaceae Effrenium voratum SCS01 Reveals Life Form Transformation Under Thermal Stress
Authors: Gong Sanqiang , Li Gang , Jin Xuejie , Qiu Dajun , Liang Jiayuan , Yu Kefu , Tan Yehui , Ma Xiao , Xia Xiaomin
Published: December 2021

Dinoflagellates in the family Symbiodiniaceae contain a number of species and play an important role in the establishment of coral reef ecosystems in oligotrophic marine waters. Effrenium voratum is likely an exclusively free-living and heterotrophic species of Symbiodiniaceae. How this species responds and acclimates to warming is largely unknown. The present study experimentally established the phenotypic landscapes related to the photobleaching and recovery processes of Effrenium voratum SCS01 following thermal stress. We found that thermal stress bleached the plastids of E. voratum SCS01 and caused the cells to become lighter in color. Thereafter, the bleached cells recovered rapidly when they returned to the optimal temperature. The dominant life form of E. voratum SCS01 shifted from mastigote cells to coccoid cells then returned to mastigote cells. Transcriptome analysis revealed that the photobleaching of E. voratum SCS01 was due to increased degradation and decreased biosynthesis of photosynthetic pigments. The thermally induced life form changes were related to the downregulation of genes for cell motility. Our results revealed the mechanism of photobleaching in E. voratum SCS01 and indicated life form transformation as a newly identified survival strategy of Effrenium voratum SCS01 under thermal stress.