bluuebunny/crossref_metadata_2025 huggingface dataset:
Channel Habits and the Development of Successful Customer-Firm Relationships in Services
Authors: Cambra-Fierro Jesús , Melero-Polo Iguácel , Patrício Lia , Sese F. Javier
Published: April 2020

Technology advances have profoundly changed the way customers and service organizations interact, leading to a multitude of service channels. This study investigates consumer habits toward service channels in order to understand the influence of these channel habits on perceptions and intentions (perceived switching costs and attitudinal loyalty) and on consumer behavior (service usage and cross-buy). We empirically test the framework in the financial services industry, and the results reveal that physical store habit increases perceived switching costs and that acquired habits toward the physical store and self-service kiosks have a positive influence on attitudinal loyalty. Perceived switching costs positively affect service usage, and attitudinal loyalty positively influences cross-buy. In addition, habits in each channel lead to an increase in the number of services acquired (cross-buy), but online and self-service kiosks channel habits negatively impact service usage, as the lack of physical presence may increase customer uncertainty. Because habits are built on the frequency and stability of channel usage, firms can manage habits by encouraging frequent interactions under stable contexts. In addition, firms should stimulate customer habits toward the physical store as it is central to the promotion of loyalty and for increasing service usage.
Fatigue, sleep disorders, anaemia and pain in the multiple sclerosis prodrome
Authors: Yusuf Fardowsa LA , Wijnands José MA , Kingwell Elaine , Zhu Feng , Evans Charity , Fisk John D , Zhao Yinshan , Sutherland Jason M , Patrick David M , Marrie Ruth Ann , Tremlett Helen
Published: April 2020

Background:

There is increasing evidence of prodromal multiple sclerosis (MS).

Objective:

The aim of this study was to determine whether fatigue, sleep disorders, anaemia or pain form part of the MS prodrome.

Methods:

This population-based matched cohort study used linked administrative and clinical databases in British Columbia, Canada. The odds of fatigue, sleep disorders, anaemia and pain in the 5 years preceding the MS cases’ first demyelinating claim or MS symptom onset were compared with general population controls. The frequencies of physician visits for these conditions were also compared. Modifying effects of age and sex were evaluated.

Results:

MS cases/controls were assessed before the first demyelinating event (6863/31,865) or MS symptom onset (966/4534). Fatigue (adj.OR: 3.37; 95% CI: 2.76–4.10), sleep disorders (adj.OR: 2.61; 95% CI: 2.34–2.91), anaemia (adj.OR: 1.53; 95% CI: 1.32–1.78) and pain (adj.OR: 2.15; 95% CI: 2.03–2.27) during the 5 years preceding the first demyelinating event were more frequent among cases, and physician visits increased for cases relative to controls. The association between MS and anaemia was greater for men; that between MS and pain increased with age. Pre-MS symptom onset, sleep disorders (adj.OR: 1.72; 95% CI: 1.12–2.56) and pain (adj.OR: 1.53; 95% CI: 1.32–1.76) were more prevalent among cases.

Conclusion:

Fatigue, sleep disorders, anaemia and pain were elevated before the recognition of MS. The relative anaemia burden was higher in men and pain more evident among older adults.
Theorising French neoliberalism: The technocratic elite, decentralised collective bargaining and France’s ‘passive neoliberal revolution’
Authors: Masquelier Charles
Published: April 2020

Despite experiencing an early and protracted neoliberal transformation, France has exhibited an acutely ambiguous stance towards neoliberal practice. This is illustrated by, for example, regular nationwide protests opposed to policies with an overtly neoliberal flavour, or the coexistence of heavy taxation and a profound financialisation of its economy. This article seeks to explain why neoliberalism successfully developed in France, despite such an ambiguity. The focus will be placed on the transformation of labour relations, which will reveal the important role played by both the technocratic elite and firm-level negotiations in legitimating neoliberal practice. It will be argued that while several relevant sociological explanations offer some valuable insights for making sense of neoliberalism’s successful development in France, Antonio Gramsci’s concept of ‘passive revolution’ provides a very fruitful basis upon which to capture the singularity of the French case.
An interactive system for humanoid robot SHFR-III
Authors: Ke Xianxin , Cao Bin , Bai Jiaojiao , Zhang Wenzhen , Zhu Yujiao
Published: April 2020

The natural interaction between human and robot is full of challenges but indispensable. In this article, a human–robot interactive system is designed for humanoid robot SHFR-III. The system consists of three subsystems: multi-sensor positioning subsystem, emotional interaction subsystem, and dialogue subsystem. The multi-sensor positioning subsystem is designed to improve the positioning accuracy, the emotional interaction subsystem uses bimodal emotional recognition model and fuzzy emotional decision-making model to realize the emotion recognition and expression feedback to the interactive objects, and the dialogue subsystem with personal information can complete the response consistent with the default information and avoid conflicting responses .The experimental results show that the multi-sensor positioning subsystem has good environmental adaptability and positioning accuracy, the emotional interaction subsystem can achieve human-like emotional feedback, and the dialogue subsystem can achieve more natural, logical, and consistent responses.
A novel short-frequency slip fault energy distribution-based demodulation technique for gear diagnosis and prognosis
Authors: Tong Shuiguang , Huang Yuanyuan , Tong Zheming , Cong Feiyun
Published: April 2020

To conduct diagnosis and prognosis of gears, this paper introduces a novel short-frequency slip fault energy distribution-based demodulation method. As an essential step of the method, the resonance-based sparse signal decomposition algorithm is firstly employed to obtain the high-resonance part from the raw gear fault signal. To deal with the difficulty in determining the resonance frequency band, we establish a multi-input signal-output model to describe the signal components acquired from a faulty gear. Based on it, the short-frequency slip fault energy distribution graph is defined to locate the center frequency. Besides, the maximum amplitude in the short-frequency slip fault energy distribution graph can be used as a health indicator for prognosis, which is named as fault-induced resonance energy ratio. The effectiveness of the proposed method is validated with both simulated signal and test data. The positive results achieved in both experiments show the perfect property of the methodology for gear fault detection with high noise, especially when the fault is incipient. In addition, by comparing the fault-induced resonance energy ratio values of faulty gears with different severity, it is proved to be a reliable health indicator for gear prognostic.
Antioxidative and Neuroprotective Effects of the Cytochalasans From Endophytes
Authors: Shen Li , Ju Jing-Jing , Liu Qin , Wang Sha-Sha , Meng Hao , Ge Xiao-Qun , Huang Wu-Yang
Published: April 2020

Six 10-indolyl-cytochalasans, chaetoglobosin F (1), chaetoglobosin Fex (2), chaetoglobosin E (3), cytoglobosin A (4), penochalasin C (5), and isochaetoglobosin D (6), and 2 10-phenyl-cytochalasans, cytochalasin H (7) and 18-methoxycytochalasin J (8) were isolated from 2 plant endophytes, Chaetomiun globosum WQ and Phomopsis sp. IFB-E060, respectively. These cytochalasans were investigated with radical-scavenging activity assay and hydrogen peroxide (H2O2)/ N-methyl-4-phenylpyridinium iodide (MPP+)-induced pheochromocytoma cell line 12 (PC12) cell models, respectively. Results showed that 7 compounds had antioxidative effects except 5, with scavenging 2,2-diphenyl-1-picrylhydrazyl radical effect 1 = 6= 7 > 2> 4 = 3 > 8 and scavenging 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical effect 1 = 6 = 7 > 2 > 3 > 8 > 4. They could also inhibit H2O2/MPP+-induced damage in PC12 cells by increasing cell viability and decreasing lactate dehydrogenase release. Compounds 1, 6, and 7 exhibited the strongest antioxidative potencies, which are more potent than vitamin E. Additionally, antioxidative and neuroprotective effects of 1∼8 showed some structure–activity relationship. These findings would be useful for looking for cytochalasin-related new lead compounds or drugs to prevent and treat Parkinson’s disease.
Making sense of climate: A meta-analytic extension of the competing values framework
Authors: Beus Jeremy M. , Solomon Shelby J. , Taylor Erik C. , Esken Candace A.
Published: April 2020

Organizational climate research has surged recently, but the disbursement of research contributions across domains has made it difficult to draw conclusions about climate and its connections with performance. To make sense of the climate literature, we used the competing values framework (CVF) to classify domain-specific climates into four climate types (clan, adhocracy, hierarchy, and market climates). We did so by conceptually linking domain-specific climates that are manifestations of the same underlying strategic values. We then conducted meta-analyses to examine the magnitudes, mechanisms, and moderators of the individual and group-level associations between the CVF climates and performance. These meta-analyses revealed positive climate–performance associations for each climate type and supported job attitudes as a common mediator. We also examined several methodological moderators of climate–performance relationships, testing the source of climate and performance measures, the temporal assessment of these constructs, and the level of within-group agreement in climate measures as possible boundary conditions.
Mapping to Quality of Life and Capability Measures in Cataract Surgery Patients: From Cat-PROM5 to EQ-5D-3L, EQ-5D-5L, and ICECAP-O Using Mixture Modelling
Authors: Dixon Padraig , Hollingworth William , Sparrow John
Published: April 2020

Objectives. Cataract is a prevalent and potentially blinding eye condition. Cataract surgery, the only proven treatment for this condition, is a very frequently undertaken procedure. The objective of this analysis was to develop a mapping algorithm that could be used to predict quality of life and capability scores from the Cat-PROM5, a newly developed, validated patient-reported outcome measure for patients undergoing cataract surgery. Methods. We estimated linear models and adjusted limited dependent variable mixture models. Data were taken from the Predict-CAT cohort of up to 1181 patients undergoing cataract surgery at two sites in England. The Cat-PROM5 was mapped to two quality of life measures (EQ-5D-3L and EQ-5D-5L) and one capability measure (ICECAP-O). All patients reported ICECAP-O and one or other of the EQ-5D measures both before and after cataract surgery. Model performance was assessed using likelihood statistics, graphical inspections of model fit, and error measurements. Results. Adjusted limited dependent variable mixture models dominated linear models on all performance criteria. Mixture models offered very good fit. Three component models that allowed component membership to be a function of covariates (age, sex, and diabetic status depending on specification and outcome measure) and which conditioned on covariates offered the best performance in almost all cases. An exception was the EQ-5D-5L post-surgery for which a two-component model was selected. Conclusions. Mapping from Cat-PROM5 to quality of life and capability measures using adjusted limited dependent variable mixture models is feasible, and the estimates can be used to support cost-effectiveness analysis in relation to cataract care. Mixture models performed strongly for both quality of life outcomes and capability outcomes.
Assessing the Vulnerability of Megaprojects Using Complex Network Theory
Authors: Guo Ning , Guo Peng , Madhavan Ravi , Zhao Jing , Liu Yang
Published: April 2020

This article examines the vulnerability of megaprojects using complex network theory. The megaproject is first abstracted as a weighted directed network, after which a novel vulnerability metric is designed to quantify megaproject vulnerability. The proposed approach is then applied to a megaproject, which demonstrates its effectiveness in assessing vulnerability and identifying critical projects. Several protection strategies are finally proposed to enhance megaproject management. Overall, this study proposes a quantitative method to assess megaproject vulnerability that can reduce vulnerability and assist in the development of proactive risk approaches to ensure successful megaproject completion.
Oxybismutomicrolite, a new pyrochlore-supergroup mineral from the Malkhan pegmatite field, Central Transbaikalia, Russia
Authors: Kasatkin Anatoly V. , Britvin Sergey N. , Peretyazhko Igor S. , Chukanov Nikita V. , Škoda Radek , Agakhanov Atali A.
Published: April 2020

Oxybismutomicrolite, ideally [(Bi3+,#)2]Σ4+Ta2O6O, where # = subordinate substituents, such as Na+, Ca2+ and vacancy (□), is a microlite-group, pyrochlore-supergroup mineral discovered at the Solnechnaya (‘Sunny’) pegmatite vein, Malkhan pegmatite field, Zabaykalskiy Kray, Central Transbaikalia, Russia. It forms rough octahedral crystals up to 1 mm across and equant grains up to 2 mm across embedded in an albite–lepidolite–elbaite complex. Other associated minerals are Bi-rich fluornatromicrolite, bismutotantalite and stibiotantalite. The new mineral is black, with resinous lustre; the streak is greyish white. It is non-fluorescent under ultraviolet light. Oxybismutomicrolite is brittle, with Mohs’ hardness of ~5. Cleavage is not observed, fracture is uneven. Dmeas. = 6.98(2) g/cm3 and Dcalc. = 7.056 g/cm3. The mineral is optically isotropic. The mean refractive index calculated from the Gladstone–Dale equation is 2.184. The infrared spectrum shows the absence of H2O molecules and OH groups. The chemical composition is (electron microprobe, wt.%): Na2O 3.45, CaO 2.88, MnO 0.31, PbO 0.76, Bi2O3 29.81, ThO2 0.18, TiO2 3.89, SnO2 1.77, Nb2O5 4.50, Ta2O5 51.08, F 1.17, O = F –0.49, total 99.31. The empirical formula, on the basis of 2 cations at the B site, is (Bi0.79Na0.68Ca0.32Mn0.03Pb0.02□0.16)Σ2.00(Ta1.42Ti0.30Nb0.21Sn0.07)Σ2.00O6.00(O0.52F0.38□0.10)Σ1.00. The crystal structure refinement (R = 0.019) gave the following data: cubic, Fd–3m, a = 10.4746(11) Å, V = 1149.2(4) Å3 and Z = 8. The eight strongest lines of the powder X-ray diffraction pattern [d, Å(I, %)(hkl)] are: 6.051(12)(111), 3.160(10)(311), 3.026(100)(222), 2.621(32)(400), 1.854(33)(440), 1.581(27)(622), 1.514(7)(444) and 1.203(7)(662). Type material is deposited in the collections of the Fersman Mineralogical Museum of the Russian Academy of Sciences, Moscow, Russia, registration number 5409/1.
Daratumumab in light chain deposition disease: rapid and profound hematologic response preserves kidney function
Authors: Milani Paolo , Basset Marco , Curci Paola , Foli Andrea , Rizzi Rita , Nuvolone Mario , Guido Raffaella , Gesualdo Loreto , Specchia Giorgina , Merlini Giampaolo , Palladini Giovanni
Published: April 2020

Key Points

Daratumumab is effective in treated light chain deposition disease. Daratumumab can prevent progression of renal failure in these patients.
The relation between peak metamorphic temperatures and subsequent cooling during continent–continent collision (western Central Alps, Switzerland)
Authors: Berger Alfons , Engi Martin , Erne-Schmid Silja , Glotzbach Christoph , Spiegel Cornelia , de Goede Rick , Herwegh Marco
Published: April 2020

The maximum temperature (Tmax) and subsequent exhumation reflect the relations between advective and conductive heat transport, which in turn depend on the tectonic evolution. To unravel these relations in an orogen, precise Tmaxdata need to be combined with relative time information for the displacements of adjacent units. We present new Tmaxdata based on Raman spectroscopy of carbonaceous material (RSCM) and zircon fission track (FT) data, which are combined with previous data and then discussed jointly. We follow this approach in the Central Alps at the western edge of the Lepontine dome. Our analysis indicates two main tectono-metamorphic domains in this area: domain A comprises the Lower Helvetic units involving the Aar Massif; domain B is situated south of the Helvetic main thrust, in the footwall of the Simplon line. In domain A, thrusted Helvetic units were overprinted mainly by reverse faulting in the Aar Massif. The thermal evolution is related to the inversion of the former Doldenhorn basin. Tectonic transport during inversion brought into contact units with substantially different Tmax. Temperature gradients were then reduced by conductive heat transfer, but thermal overprinting during cooling involved subsequent vertical movements as well. Zircon FT data yield apparent ages between 12 and 18 Ma in the external part, but 8–9 Ma in the internal part of the Aar Massif. The youngest ages are taken as the cooling at a given temperature, whereas the other data are discussed as being only partially resetted along a temperature path in the partial annealing zone of the zircon FT. When combined with age data for Tmaxand apatite FT data from the literature, the youngest group exhibits exhumation rates between 0.5 and 1.2 km/Ma in the time range between 20 Ma and today. In all of domain B, Tmaxwas significantly higher than in domain A. In domain B the estimated rates of exhumation are 0.8–1.0 km/Ma for the post-20 Ma time interval. Despite of different temperature evolution, the exhumation rates are similar in both domains. The study shows the necessity to combine detailed tectonic data to interpret the T–t evolution of such an area.
Association between FCGR2A rs1801274 and MUC5B rs35705950 variations and pneumonia susceptibility
Authors: Shi Xueshu , Ma Yue , Li Haiyan , Yu Huanxin
Published: April 2020

Background

Herein, we collected currently published data to comprehensively evaluate the impact of the FCGR2A (Fc fragment of IgG receptor IIa) rs1801274 and MUC5B (mucin 5B, oligomeric mucus/gel-forming) rs35705950 variations on susceptibility to pneumonia diseases.

Methods

We retrieved case-control studies from three online databases and applied the statistical approach of meta-analysis for a series of pooling analyses.

Results

A total of fourteen case-control studies were included for FCGR2A rs1801274; while thirty-one case-control studies were included for MUC5B rs35705950. No significant difference between pneumonia cases and controls for FCGR2A rs1801274 was found. However, MUC5B rs35705950 was significantly associated with pneumonia susceptibility in the whole population under the genetic models of allelic T vs. G [OR (odds ratio) =3.78], carrier T vs. G (OR = 3.31), TT vs. GG (OR = 13.66), GT vs. GG (OR = 4.78), GT + TT vs. GG (OR = 5.05), and TT vs. GG + GT (OR = 6.47) (all P < 0.001, Bonferroni-adjusted P < 0.006; false discovery rate-adjusted P < 0.0010). Furthermore, we observed a similar positive result for subgroup analyses of “Caucasian”, “Asian”, “population-based control”, and “idiopathic pulmonary fibrosis”.

Conclusions

MUC5B rs35705950, but not FCGR2A rs1801274, increases susceptibility to clinical pneumonia, especially to idiopathic pulmonary fibrosis, in both the Caucasian and Asian populations.
Tranexamic acid is associated with reduced mortality, hemorrhagic expansion, and vascular occlusive events in traumatic brain injury – meta-analysis of randomized controlled trials
Authors: July Julius , Pranata Raymond
Published: April 2020

Background

This systematic review and meta-analysis aimed to synthesize the latest evidence on the efficacy and safety of tranexamic acid (TXA) on traumatic brain injury (TBI).

Methods

We performed a systematic literature search on topics that compared intravenous TXA to placebo in patients with TBI up until January 2020 from several electronic databases.

Results

There were 30.522 patients from 7 studies. Meta-analysis showed that TXA was associated with reduced mortality (RR 0.92 [0.88, 0.97], p = 0.002; I2: 0%) and hemorrhagic expansion (RR 0.79 [0.64, 0.97], p = 0.03; I2: 0%). Both TXA and control group has a similar need for neurosurgical intervention (p = 0.87) and unfavourable Glasgow Outcome Scale (GOS) (p = 0.59). The rate for vascular occlusive events (p = 0.09), and its deep vein thrombosis subgroup (p = 0.23), pulmonary embolism subgroup (p = 1), stroke subgroup (p = 0.38), and myocardial infarction subgroup (p = 0.15) were similar in both groups. Subgroup analysis on RCTs with low risk of bias showed that TXA was associated with reduced mortality and hemorrhagic expansion. TXA was associated with reduced vascular occlusive events (RR 0.85 [0.73, 0.99], p = 0.04; I2: 4%). GRADE was performed for the RCT with low risk of bias subgroup, it showed a high certainty of evidence for lower mortality, less hemorrhage expansion, and similar need for neurosurgical intervention in TXA group compared to placebo group.

Conclusion

TXA was associated with reduced mortality and hemorrhagic expansion but similar need for neurosurgical intervention and unfavorable GOS. Vascular occlusive events were slightly lower in TXA group on subgroup analysis of RCTs with low risk of bias.
Sleep quality improves during treatment with repetitive transcranial magnetic stimulation (rTMS) in patients with cocaine use disorder: a retrospective observational study
Authors: Gómez Pérez Luis J. , Cardullo Stefano , Cellini Nicola , Sarlo Michela , Monteanni Tommaso , Bonci Antonello , Terraneo Alberto , Gallimberti Luigi , Madeo Graziella
Published: April 2020

Background

Sleep disturbance is a prominent and common complaint in people with cocaine use disorder (CUD), either during intake or withdrawal. Repetitive transcranial magnetic stimulation (rTMS) has shown promise as a treatment for CUD. Thus, we evaluated the relationship between self-perceived sleep quality and cocaine use pattern variables in outpatients with CUD undergoing an rTMS protocol targeted at the left dorsolateral prefrontal cortex.

Methods

This is a retrospective observational study including 87 patients diagnosed with CUD according to the DSM-5 criteria. Scores in Pittsburgh Sleep Quality Index (PSQI), Cocaine Craving Questionnaire (CCQ), Beck Depression Inventory-II (BDI-II), Self-rating Anxiety Scale (SAS), and Symptoms checklist 90-Revised (outcome used: Global Severity Index, GSI) were recorded at baseline, and after 5, 30, 60, and 90 days of rTMS treatment. Cocaine use was assessed by self-report and regular urine screens.

Results

Sleep disturbances (PSQI scores> 5) were common in patients at baseline (mean ± SD; PSQI score baseline: 9.24 ± 3.89; PSQI> 5 in 88.5% of patients). PSQI scores significantly improved after rTMS treatment (PSQI score Day 90: 6.12 ± 3.32). Significant and consistent improvements were also seen in craving and in negative-affect symptoms compared to baseline. Considering the lack of a control group, in order to help the conceptualization of the outcomes, we compared the results to a wait-list group (n = 10). No significant improvements were observed in the wait-list group in any of the outcome measures.

Conclusions

The present findings support the therapeutic role of rTMS interventions for reducing cocaine use and accompanying symptoms such as sleep disturbance and negative-affect symptoms.

Trial registration

ClinicalTrials.gov.NCT03733821.
Physical activity in paid work time for desk-based employees: a qualitative study of employers’ and employees’ perspectives
Authors: Ryde Gemma C. , Atkinson Patricia , Stead Martine , Gorely Trish , Evans Josie M. M.
Published: April 2020

Background

Poor physical and mental health of employees create significant problems in the workplace. Physical activity (PA) has been shown as an effective strategy for preventing and treating numerous physical and mental health issues as well as work performance outcomes. However, there are many barriers to taking part in PA (such as lack of time) with participation rates typically low. Providing PA in paid work time might be a way to overcome these issues, yet employers’ and employees’ opinions of this concept are unknown. The aim of this study was to explore employee and employer perspectives of PA in paid work time.

Methods

Workplaces were recruited through existing contacts on the research team. Focus groups and interviews were conducted with employees and managers at one University and two executive non-departmental public bodies in central Scotland with mainly desk-based employees. Both managers and employees were involved to gain perspectives throughout the organisational hierarchy and were interviewed separately to reduce social desirability bias. All discussions were digitally recorded and transcribed verbatim. Data were analysed thematically for both managers and employees but due to significant overlap in themes between the groups, these are reported together in the results.

Results

Three out of five organisations approached took part in this qualitative study. Two individual interviews were held with strategic managers, five focus groups with middle managers (n = 16) and nine with employees (n = 45). Benefits were anticipated by managers and employees for both employees themselves and the organisation and included improved mental health, productivity and more favourable perceptions of the employer. Despite these widely acknowledged benefits, significant barriers were identified and included the structure and nature of the working day (high workload, front line job requirements), workplace culture and norms (resentment from colleagues, no break culture) and organisational concerns (cost of lost time, public perceptions).

Conclusion

This study suggests that there are significant barriers to PA in paid work time. Whilst numerous anticipated benefits were conveyed by both employees and managers, PA in paid work time is unlikely to become common place until changes in attitudes and the culture towards movement at work occur.
Socio-demographic characteristics and associated factors influencing cervical cancer screening among women attending in St. Paul’s Teaching and Referral Hospital, Ethiopia
Authors: Woldetsadik Abebe Belete , Amhare Abebe Feyissa , Bitew Sintayehu Tsegaye , Pei Leilei , Lei Jian , Han Jing
Published: April 2020

Background

In Ethiopia, cervical cancer is the second most frequent cancer among women aged 15 to 44 years old. Cervical cancer screening is an effective measure to enhance the early detection of cervical cancer for prevention. However, the magnitude of cervical cancer screening is less than 1%. This study aimed to determine the influence of sociodemographic characteristics and related factors on screening.

Method

A hospital-based cross-sectional study has been conducted from July to September 2017. Data have been collected using interviewer-administered questioner among 425 women (18–49 years age) who visited the family health department at St. Paul’s Hospital. Descriptive statistics, chi-square, univariate and multivariate logistic regression were used for data analysis.

Result

Of the 425 study participants, only 12.2% of women have been screened within the past 3 years. Women in the age range of 40–49 years old were more likely to be screened (36.1%) than women age 18–29 years (8%). Women living in urban were more likely to be screened (15.9%) than women living in rural (3.9%). Other factors including low monthly income, unlikely chance of having cancer, lack of knowledge, and fear test outcome were significantly associated with the low uptake of screening.

Conclusion

This study revealed that the uptake of cervical cancer screening was low. Women in the potential target population of cervical cancer screening were just a proportion of all studied age groups and screening in them was more common than in younger women. Besides, rural residence, low monthly income, and lack of knowledge were important predictors for low utilization of cervical cancer screening practice.
Health and economic burden of respiratory syncytial virus (RSV) disease and the cost-effectiveness of potential interventions against RSV among children under 5 years in 72 Gavi-eligible countries
Authors: Li Xiao , Willem Lander , Antillon Marina , Bilcke Joke , Jit Mark , Beutels Philippe
Published: April 2020

Background

Respiratory syncytial virus (RSV) frequently causes acute lower respiratory infection in children under 5, representing a high burden in Gavi-eligible countries (mostly low-income and lower-middle-income). Since multiple RSV interventions, including vaccines and monoclonal antibody (mAb) candidates, are under development, we aim to evaluate the key drivers of the cost-effectiveness of maternal vaccination and infant mAb for 72 Gavi countries.

Methods

A static Multi-Country Model Application for RSV Cost-Effectiveness poLicy (MCMARCEL) was developed to follow RSV-related events monthly from birth until 5 years of age. MCMARCEL was parameterised using country- and age-specific demographic, epidemiological, and cost data. The interventions’ level and duration of effectiveness were guided by the World Health Organization’s preferred product characteristics and other literature. Maternal vaccination and mAb were assumed to require single-dose administration at prices assumed to align with other Gavi-subsidised technologies. The effectiveness and the prices of the interventions were simultaneously varied in extensive scenario analyses. Disability-adjusted life years (DALYs) were the primary health outcomes for cost-effectiveness, integrated with probabilistic sensitivity analyses and Expected Value of Partially Perfect Information analysis.

Results

The RSV-associated disease burden among children in these 72 countries is estimated at an average of 20.8 million cases, 1.8 million hospital admissions, 40 thousand deaths, 1.2 million discounted DALYs, and US$611 million discounted direct costs. Strategy ‘mAb’ is more effective due to its assumed longer duration of protection versus maternal vaccination, but it was also assumed to be more expensive. Given all parameterised uncertainty, the optimal strategy of choice tends to change for increasing willingness to pay (WTP) values per DALY averted from the current situation to maternal vaccination (at WTP > US$1000) to mAB (at WTP > US$3500). The age-specific proportions of cases that are hospitalised and/or die cause most of the uncertainty in the choice of optimal strategy. Results are broadly similar across countries.

Conclusions

Both the maternal and mAb strategies need to be competitively priced to be judged as relatively cost-effective. Information on the level and duration of protection is crucial, but also more and better disease burden evidence—especially on RSV-attributable hospitalisation and death rates—is needed to support policy choices when novel RSV products become available.
A novel fungal beta-propeller phytase from nematophagous Arthrobotrys oligospora: characterization and potential application in phosphorus and mineral release for feed processing
Authors: Hou Xianjuan , Shen Zhen , Li Na , Kong Xiaowei , Sheng Kangliang , Wang Jingmin , Wang Yongzhong
Published: April 2020

Phytases are widely utilized in feed industry to increase the utilization of phosphorus, minerals, and amino acids for improvement of animal and human nutrition. At present, all known β-propeller phytases (BPP) have been generated from bacteria, particularly Bacillus. In this work we report for the first time a new fungal-derived BPP phytase. We identified a phytase highly differentially expressed only in the parasitic stage of a nematophagous fungus, Arhtrobotrys oliogospora, during the development of the 3D traps. We found that this phytase was homologous to the known bacterial BPP phytase, thus we referred the new phytase to Aophytase. The heterologous expression of codon-optimized Aophytase gene in Pichia pastoris was successfully investigated to yield recombinant Aophytase (r-Aophytase) with high specific enzyme activity of 74.71 U/mg, much higher than those of recombinant BPP phytases derived bacteria. The kinetic parameters of the r-Aophytase, the optimum pH and temperature, as well as the effects of surfactant, EDTA and different ions on its enzyme activity were further investigated. The potential utilization of r-Aophytase in feed processing was finally explored. We found that the optimal pH value was about 7.5, and the optimal temperature was 50 °C.; r-Aophytase significantly increased the release of inorganic phosphorus from soybean meal, and improved the release of soluble minerals from the durum wheat flour and finger millet flour. The findings indicate its potential utilization in the feed processing to ameliorate nutritional value of cereals and animal feed in the future.
The effects of statins on dental and oral health: a review of preclinical and clinical studies
Authors: Tahamtan Shabnam , Shirban Farinaz , Bagherniya Mohammad , Johnston Thomas P. , Sahebkar Amirhossein
Published: April 2020

The statin family of drugs are safe and effective therapeutic agents for the treatment of arteriosclerotic cardiovascular disease (CVD). Due to a wide range of health benefits in addition to their cholesterol lowering properties, statins have recently attracted significant attention as a new treatment strategy for several conditions, which are not directly related to normalizing a lipid profile and preventing CVD. Statins exert a variety of beneficial effects on different aspects of oral health, which includes their positive effects on bone metabolism, their anti-inflammatory and antioxidant properties, and their potential effects on epithelization and wound healing. Additionally, they possess antimicrobial, antiviral, and fungicidal properties, which makes this class of drugs attractive to the field of periodontal diseases and oral and dental health. However, to the best of our knowledge, there has been no comprehensive study to date, which has investigated the effects of statin drugs on different aspects of dental and oral health. Therefore, the primary objective of this paper was to review the effect of statins on dental and oral health. Results of our extensive review have indicated that statins possess remarkable and promising effects on several aspects of dental and oral health including chronic periodontitis, alveolar bone loss due to either extraction or chronic periodontitis, osseointegration of implants, dental pulp cells, orthodontic tooth movement, and orthodontic relapse, tissue healing (wound/bone healing), salivary gland function, and finally, anti-cancer effects. Hence, statins can be considered as novel, safe, inexpensive, and widely-accessible therapeutic agents to improve different aspects of dental and oral health.
Benefits and limitations of humanized mice in HIV persistence studies
Authors: Marsden Matthew D.
Published: April 2020

Significant advances in the treatment of HIV infection have been made in the last three decades. Antiretroviral therapy (ART) is now potent enough to prevent virus replication and stop disease progression. However, ART alone does not cure the infection, primarily because HIV can persist in stable long-term reservoir cells including latently-infected CD4 + T cells. A central goal of the HIV research field is to devise strategies to eliminate these reservoirs and thereby develop a cure for HIV. This requires robust in vivo model systems to facilitate both the further characterization of persistent HIV reservoirs and evaluation of methods for eliminating latent virus. Humanized mice have proven to be versatile experimental models for studying many basic aspects of HIV biology. These models consist of immunodeficient mice transplanted with human cells or tissues, which allows development of a human immune system that supports robust infection with HIV. There are many potential applications for new generations of humanized mouse models in investigating HIV reservoirs and latency, but these models also involve caveats that are important to consider in experimental design and interpretation. This review briefly discusses some of the key strengths and limitations of humanized mouse models in HIV persistence studies.
Analysis of reassortant and intragenic recombination in Cypovirus
Authors: Zhang Zhendong , Li Ning , Hou Chengxiang , Gao Kun , Tang Xudong , Guo Xijie
Published: April 2020

Cypoviruses (CPVs) are RNA viruses with segmented double-stranded genome and major pathogens of various insects, including economic insects like silkworms and pest insects for agricultural crops and forests. Genome reassortment and recombination are common phenomenon for viruses as a mechanism to expand host range and increase virulence. In the present study, we analyzed the reassortant and recombination events for CPVs. The results showed that two genome segments (S1 and S4) of BmCPV1-YN shared higher nucleotide identity with the corresponding segment of BmCPV1-I while others were all more closely to BmCPV1-SZ, suggesting BmCPV1-YN was originated from reassortant events between BmCPV1-I and BmCPV1-SZ. Recombination analyses revealed that S6 of BmCPV1-YN was a recombinant segment derived from BmCPV1-I and BmCPV1-SZ, and S10 of DpCPV1 was a recombinant segment emerged from BmCPV1-I and LdCPV1. Our findings provide the evidence for the fact that CPVs could undergo reassortant and recombinant events and enrich the knowledge about etiology and molecular epidemiology of CPVs.
Comparison of CTVHR and organs at risk contours between TRUS and MR images in IB cervical cancers: a proof of concept study
Authors: Mendez Lucas C. , Ravi Ananth , Martell Kevin , Raziee Hamid , Alayed Yasir , Wronski Matt , Paudel Moti , Barnes Elizabeth , Taggar Amandeep , Wong C. S. , Leung Eric
Published: April 2020

Purpose

To compare CTVHR and OAR dimensions and inter-rater agreement between magnetic resonance (MR) and trans-rectal ultrasound (TRUS) images in IB cervical cancer patients.

Methods

IB cervical cancer patients treated with (chemo)radiotherapy plus MR-guided brachytherapy (BT) were prospectively enrolled in this study. Radiation oncologists contoured CTVHR and OARs in pre-BT MR images (MRI) and intra-operative TRUS images. These contours were subsequently compared in regard to volume and dimension. Contour inter-rater agreement analysis was also investigated using kappa index (KI). Stata 15.0 was used for statistical analysis and a p-value < 0.05 was considered statistically significant.

Results

TRUS CTVHR volumes were statistically smaller than the respective MRI contoured volumes. TRUS CTVHR thickness was found to be consistently smaller than MRI contours in all patients. No statistical difference was seen in width and height between the two different imaging modalities. MRI contours had a median KI of 0.66 (range: 0.56–0.77) while TRUS-based contours had a median KI of 0.64 (range: 0.47–0.77). Bladder and rectum had very satisfactory KI in both imaging modalities. Vaginal contours had moderate agreement in MR (0.52) and in TRUS images (0.58).

Conclusion

TRUS images allow good visualization of CTVHR and OARs in IB cervical cancer patients. Inter-rater contour variability was comparable between TRUS and MR images. TRUS is a promising modality on its own for image-guided BT.
Impact of adjuvant radiotherapy on the survival of women with optimally resected stage III endometrial cancer in the era of modern radiotherapy: a retrospective study
Authors: Chen Jenny Ling-Yu , Huang Yu-Sen , Huang Chao-Yuan , Hsu Che-Yu , Lan Keng-Hsueh , Cheng Wen-Fang , Kuo Sung-Hsin
Published: April 2020

Background

The optimal adjuvant treatment for stage III endometrial cancer in the era of modern radiotherapy remains undefined. We investigated the benefit of adjuvant radiotherapy for women who underwent optimal resection for stage III endometrial cancer in the era of modern radiotherapy.

Methods

We retrospectively reviewed patients with endometrial cancer who were treated between 2010 and 2018. Adjuvant treatment included radiotherapy by modern radiotherapy techniques (intensity-modulated or volumetric modulated arc radiotherapy), chemotherapy, or both. Recurrence-free survival (RFS) and overall survival (OS) were calculated using the Kaplan-Meier method and analyzed via multivariate Cox proportional hazards models.

Results

One hundred sixty-one patients were initially included (52, 9, and 100 with stages IIIA, IIIB, and IIIC cancer, respectively); 154 patients (96%) received adjuvant therapy. Such adjuvant treatment was associated with improved RFS (p = 0.014) and OS (p = 0.044) over surgery alone. Adjuvant radiotherapy by modern radiotherapy techniques led to low incidence of acute (25%) and chronic (7%) grade ≥ 2 gastrointestinal toxicity. On univariate analysis, non-endometrioid histology and grade 3 status were associated with higher risks of tumor recurrence and death, whereas adjuvant radiotherapy alone or in combination chemotherapy reduced their risks. On multivariate analysis, non-endometrioid histology was associated with increased recurrence (hazard ratio [HR], 2.95; p = 0.009), whereas adjuvant radiotherapy alone or with chemotherapy was associated with lower recurrence (HR, 0.62; p = 0.042). Patients > 60 years of age (p = 0.038) as well as those with endometrioid histology (p = 0.045), lymphovascular space invasion (p = 0.031), and ≥ 2 positive lymph nodes (p = 0.044) benefited most from adjuvant radiotherapy.

Conclusions

Modern adjuvant radiotherapy (intensity-modulated or volumetric modulated arc radiotherapy) alone or with chemotherapy should be considered for women with optimally resected stage III endometrial cancer.

Trial registration

ClinicalTrials.gov, NCT04251676. Registered 24 January 2020. Retrospectively registered.
MicroRNA-1202 plays a vital role in osteoarthritis via KCNQ1OT1 has-miR-1202-ETS1 regulatory pathway
Authors: Liu Changzeng , Gao Jianming , Su Guangyan , Xiang Yang , Wan Le
Published: April 2020

Background

This study aimed to explore the molecular mechanism of osteoarthritis (OA) and provide information about new genes as potential targets for OA treatment.

Methods

Gene expression profile of GSE105027, including 12 OA serum samples (OA group) and 12 healthy serum samples (ctrl group), was downloaded. The differentially expressed miRNAs (DEMs) as well as miRNA-mRNAs interactions were investigated, followed by function and pathway investigation. Then the protein-protein interaction (PPI) network was performed. Furthermore, the long non-coding RNA (lncRNA)-miRNA-mRNA interactions (competing endogenous RNAs, ceRNAs) were investigated.

Results

A total of 17 downregulated miRNAs were revealed between OA and ctrl groups. These DEMs such as has-miR-1202 were mainly enriched in GO functions like histone acetyltransferase binding and KEGG pathways like cellular senescence. The integrated PPI network analysis showed that has-miR-1202, has-miR-33b-3p, has-miR-940, has-miR-4284, and has-miR-4281 were 5 downregulated miRNAs in this network. Furthermore, the lncRNA-miRNA-mRNA interactions such as KCNQ1OT1-has-miR-1202-ETS1 were revealed in the present ceRNA network.

Conclusion

Key DEMs such as miR-33b-3p, miR-940, and miR-1202 may be involved in OA. miR-1202 may regulate OA development via histone acetyltransferase pathway binding function and cellular senescence pathway. Furthermore, KCNQ1OT1-has-miR-1202-ETS1 might be vital for the process of OA.
Identification of a novel Shank2 transcriptional variant in Shank2 knockout mouse model of autism spectrum disorder
Authors: Lee Yong-Seok , Yu Nam-Kyung , Chun Jeewan , Yang Jung-eun , Lim Chae-Seok , Kim Hyopil , Park Gaeun , Lee Jin-A , Lee Kyungmin , Kaang Bong-Kiun , Lee Jae-Hyung
Published: April 2020

Autism spectrum disorder (ASD) is a group of neurodevelopmental disorders that are highly heterogeneous in clinical symptoms as well as etiologies. Mutations in SHANK2 are associated with ASD and accordingly, Shank2 knockout mouse shows ASD-like behavioral phenotypes, including social deficits. Intriguingly, two lines of Shank2 knockout (KO) mouse generated by deleting different exons (exon 6–7 or exon 7) showed distinct cellular phenotypes. Previously, we compared gene expressions between Shank2 KOs lacking exon 6–7 (e6–7 KO) and KOs lacking exon 7 (e7 KO) by performing RNA-seq. In this study, we expanded transcriptomic analyses to identify novel transcriptional variants in the KO mice. We found prominent expression of a novel exon (exon 4′ or e4’) between the existing exons 4 and 5 in the Shank2 e6–7 KO model. Expression of the transcriptional variant harboring this novel exon was confirmed by RT-PCR and western blotting. These findings suggest that the novel variant may function as a modifier gene, which contributes to the differences between the two Shank2 mutant lines. Furthermore, our result further represents an example of genetic compensation that may lead to phenotypic heterogeneity among ASD patients with mutations in the same gene.
Cullin-7 (CUL7) is overexpressed in glioma cells and promotes tumorigenesis via NF-κB activation
Authors: Xu Jianye , Zhang Zongpu , Qian Mingyu , Wang Shaobo , Qiu Wei , Chen Zihang , Sun Zhongzheng , Xiong Ye , Wang Chaochao , Sun Xiaopeng , Zhao Rongrong , Xue Hao , Li Gang
Published: April 2020

Background

Cullin-7 (CUL7) is a member of the DOC domain-containing cullin family and is involved in the regulation of cell transformation. However, the clinical significance, potential mechanism and upstream regulators of CUL7 in malignant gliomas remain to be determined.

Methods

Expression level data and clinical information were obtained via the Cancer Genome Atlas (TCGA) database, the Chinese Glioma Genome Atlas (CGGA) database, immunohistochemistry (IHC) and western blot analysis. Gene set enrichment analysis (GSEA) was used to explore the potential molecular mechanisms of CUL7. RNA silencing was performed using siRNA or lentiviral constructs in U87MG and U251 glioma cell lines and GSC267 glioma stem cells. CUL7 overexpression was performed using the GV141-CUL7 plasmid construct. In addition, overexpression of miR-3940-5p was performed and validated by quantitative real-time PCR (qRT-PCR). Cells were characterized in vitro or in vivo to evaluate their molecular status, cell proliferation, invasion, and migration by Cell Counting Kit (CCK)-8, EdU, flow cytometry, colony formation, Transwell and 3D tumour spheroid invasion assays. Coimmunoprecipitation (co-IP) and western blotting were performed to test the mechanisms of activation of the NF-κB signalling pathway.

Results

High CUL7 expression was associated with a high tumour grade, a mesenchymal molecular glioma subtype and a poor prognosis in patients. Gene silencing of CUL7 in U87MG and U251 cells significantly inhibited tumour growth, invasion and migration in vitro and in vivo. Western blot analysis revealed that cyclin-dependent kinase inhibitors and epithelial-mesenchymal transition (EMT) molecular markers changed under CUL7 silencing conditions. In contrast, CUL7 overexpression promoted tumour growth, invasion and migration. Gene set enrichment analysis (GSEA) and western blot analysis revealed that CUL7 was positively associated with the NF-κB pathway. Moreover, with coimmunoprecipitation assays, we discovered that CUL7 physically associated with MST1, which further led to ubiquitin-mediated MST1 protein degradation, which promoted activation of the NF-κB signalling pathway. Finally, CUL7 was found to be downregulated by miR-3940-5p, which suppressed the development of gliomas.

Conclusions

Our findings indicate that CUL7 plays a significant role in promoting tumorigenesis via NF-κB activation and that it can be negatively regulated by miR-3940-5p in human gliomas. Furthermore, CUL7 might be a candidate molecular target for the treatment of glioma.