bluuebunny/crossref_metadata_2025 huggingface dataset:
Continuous monitoring of the health status of cement-based structures: electrical impedance measurements and remote monitoring solutions
Authors: Giulietti Nicola , Chiariotti Paolo , Cosoli Gloria , Giacometti Giovanni , Violini Luca , Mobili Alessandra , Pandarese Giuseppe , Tittarelli Francesca , Revel Gian Marco
Published: January 2022

The continuous monitoring of cement-based structures and infrastructures is fundamental to optimize their service life and reduce maintenance costs. In the framework of the EnDurCrete project (GA no. 760639), a remote monitoring system based on electrical impedance measurements was developed. Electrical impedance is measured according to the Wenner’s method, using 4-electrode arrays embedded in concrete during casting, selecting alternating current as excitation, to avoid the polarization of both electrode/material interface and of material itself. With this measurement, it is possible to promptly identify events related to contaminants ingress or damages (e.g. cracks formation). Conductive additions are included in some elements to enhance signal-to-noise ratio, as well as the self-sensing properties of concrete. Specifically, a distributed sensor network was implemented, consisting of measurement nodes installed in the elements to be monitored, then connected to a central hub (RS-232 protocol). Nodes are realized with an embedded unit for electrical impedance measurements (EVAL-AD5940BIOZ board with AD5940 chip, by Analog Device) and a digital thermometer (DS18B20 by Maxim Integrated), enclosed in cabinets filled with an IP68 gel against moist-related problems. Data are available on a Cloud through Wi-Fi network or LTE modem, hence can be accessed remotely via a use-friendly multi-platform interface.
Occupancy grid mapping for rover navigation based on semantic segmentation
Authors: Chiodini Sebastiano , Pertile Marco , Debei Stefano
Published: January 2022

Obstacle mapping is a fundamental building block of the autonomous navigation pipeline of many robotic platforms such as planetary rovers. Nowadays, occupancy grid mapping is a widely used tool for obstacle perception. It foreseen the representation of the environment in evenly spaced cells, whose posterior probability of being occupied is updated based on range sensors measurement. In more classic approaches, the cells are updated to occupied at the point where the ray emitted by the range sensor encounters an obstacle, such as a wall. The main limitation of this kind of methods is that they are not able to identify planar obstacles, such as slippery, sandy, or rocky soils. In this work, we use the measurements of a stereo camera combined with a pixel labeling technique based on Convolution Neural Networks to identify the presence of rocky obstacles in planetary environment. Once identified, the obstacles are converted into a scan-like model. The estimation of the relative pose between successive frames is carried out using ORB-SLAM algorithm. The final step consists of updating the occupancy grid map using the Bayes’ update Rule. To evaluate the metrological performances of the proposed method images from the Martian analogous dataset, the ESA Katwijk Beach Planetary Rover Dataset have been used. The evaluation has been performed by comparing the generated occupancy map with a manually segmented ortomosaic map, obtained by drones’ survey of the area used as reference.
Thermoelasticity and ArUco marker-based model validation of polymer structure: application to the San Giorgio’s bridge inspection robot
Authors: Capponi Lorenzo , Tocci Tommaso , D'Imperio Mariapaola , Abidi Syed Haider Jawad , Scaccia Massimiliano , Cannella Ferdinando , Marsili Roberto , Rossi Gianluca
Published: January 2022

Experimental procedures are often involved in the numerical models validation. To define the behaviour of a structure, its underlying dynamics and stress distributions are generally investigated. In this research, a multi-instrumental and multi-spectral method is proposed in order to validate the numerical model of the Inspection Robot mounted on the new San Giorgio's Bridge on the Polcevera river. An infrared thermoelasticity-based approach is used to measure stress-concentration factors and, additionally, an innovative methodology is implemented to define the natural frequencies of the Robot Inspection structure, based on the detection of ArUco fiducial markers. Established impact hammer procedure is also performed for the validation of the results.
Strategi Membangun Kewirausahaan Santri Menggunakan Model Pendidikan Taxonomi Bloom
Authors: Mun'im Muhammad , Pardiman Pardiman , Supriyanto Supriyanto
Published: January 2022

Pesantren Pesantren ialah diantara dari sekian satu banyak dari lembaga pendidikan yang berada di Indonesia yang di dalamnya mentransfer ilmu agama Islam pada santri-santrinya. selain dibekali ilmu agama santri juga dipercaya mampu menghadapi tantangan zaman, bahkan santri juga bisa untuk membuka lapangan pekerjaan serta menjadi santripreneur yang sukses, dengan diberikan pendidikan kewirausahaan kepada santri. Penelitian ini bertujuan untuk menganalisis bagaimana strategi pendidikan yang diberikan kepada santri beserta implikasinya. Metode penelitian memakai riset kualitatif dengan pendekatan deskriptif serta rancangan multisitus. Metode pengumpulan datanya dalam penelitian ini yaitu menggunakan pewawancaraan, pengobservasani serta pendokumentasian dengan pengecekan keabsahan data yang menggunakan metode triangulasi. Penelitian ini memakai teknik analisis data deskriptif kualitatif dengan mereduksi data, penyajian data verifikasi dan penarikan simpulan. Dari hasil penelitian bersimpulan bahwa dalam membangun entrepreneurship santri di Pondok Pesantren Al-Mawaddah Kudus serta Pesantren Raudlatun Nasyiin Rembang yaitu 1) memberikan pendidikan kewirausahaan secara kognitif yang didalamnya santri diberi pengetahuan dan pemahaman mengenai ilmu kewirausahaan. 2) santri diberikan pendidikan kewirausahaan secara afektif yang didalamnya terdapat pemberian pelatihan, motivasi dan nilai-nilai kewirausahaan. 3) selain dibekali pendidikan kognitif dan afektif santri juga dibekali pendidikan psikomotorik untuk membentuk mental dan keterampilan santri dengan cara praktik langsung dilapangan dengan berbagai kegiatan kewirausahaan seperti menjual barang dan jasa.
Pengaruh Nilai Tukar dan Inflasi pada Reksadana Syariah di Indonesia
Authors: Hartati Yuliana Eva , Kismawadi Early Ridho , Hamid Abdul , Mardhiah Ainun
Published: January 2022

The increasing development of Islamic mutual funds and making a more varied and more promising sharia capital market instrument for investors who want to invest their capital in the Islamic capital market, are able to make Indonesia the largest country in establishing a sharia capital market. However, the development of Islamic mutual funds is not easy to make the largest sharia-based investment container in Indonesia because there are still many factors that can affect the rise and fall of NAVs in Islamic mutual funds that Investment Managers still find difficult to overcome. The purpose of this study is to analyze the factors that influence the development of Islamic mutual funds in Indonesia. In this study the data used is secondary data. Data obtained from various sources, namely part of the Indonesia Stock Exchange (BEI) Annual Report and also a portion of the Sharia Mutual Fund Development Statistics in the Financial Services Authority (OJK) and Bank Indonesia (BI) taken in the third quarter of 2015-Quarterly III 2018. Data were analyzed using multiple linear regression methods and using SPSS software. The results obtained showed that partially Islamic NAV mutual funds were only influenced by inflation and the number of RDS, while the Jakarta Islamic Indeks and the exchange rate did not affect the NAV of Islamic mutual funds. Simultaneously shows that Jakarta Islamic Indeks, exchange rate, inflation and the amount of RDS have a simultaneous influence on the dependent variable (Islamic mutual fund NAV.
Comparative assessment of mortality and lethality rates of newborns at the regional level
Authors: Ivanov D. O. , Yuryev V. K. , Petrenko Yu. V. , Moiseeva K. E. , Mogileva I. I. , Mezhidov K. S. , Alekseeva A. V. , Berezkina E. N. , Harbediya Sh. D. , Daineko M. Yu. , Glushchenko V. A.
Published: January 2022

In order to assess the mortality and lethality rates of newborns in obstetric organizations of the North-Western Federal District in 2013–2019, a comparative analysis of official statistics data was carried out. It was found that in the North-Western Federal District in the period from 2013 to 2019, there was an almost annual decrease in newborn mortality rates. The overall decrease in mortality in maternity care organizations of the Federal District (from 2,5% to 1,9%) was mainly due to a decrease in mortality in obstetric hospitals of the first and second levels (respectively from 3,2% to 1,2% and from 1,7% to 0,7%), while most children died in perinatal centers, where the mortality rate has not changed in recent years (2013 – 4,1%; 2019 – 4,0%). The study showed a decrease in mortality rates in the subjects of the Russian Federation that are part of the federal district, except for the city of St. Petersburg. The average hospital lethality rate of newborns in maternity care organizations of the North-Western Federal District during 2013–2019 was in the range of 1,9%–2,0%, did not change significantly and corresponded to the national average. However, the level of hospital lethality significantly differed in individual subjects of the district – in more than half of them, the level of hospital lethality exceeded the average, while in others it was significantly lower. Thus, the decrease in the mortality and lethality rates of newborns in maternity care organizations indicates an increase in the quality of medical care for children in the North-Western Federal District. dicates an increase in the quality of medical care for children in the North-Western Federal District.
Ecological and hygienic problems in the Arctic territories of intensive industrial activity (review)
Authors: Gudkov A. B. , Degteva G. N. , Shepeleva O. A.
Published: January 2022

The Goal. To analyze the literature on the impact of high latitude climate on human health, as well as consider some aspects of adaptation to Arctic conditions and nutritional issues for the population and shift workers.

Methods. Review of the articles placed in the bibliographic and abstract databases of VINITI, CyberLeninka, RSCI, Scopus, WoS, on the study of environmental and hygienic problems in the Arctic territories, where intense industrial activity is carried out. The following keywords were used: Arctic territories, adaptation, watch, food.

Results. Long-term fundamental research has established that a complex of unfavorable and extreme natural and climatic factors in the Arctic causes two main syndromes in humans – “polar tension” and “northern tissue hypoxia”. Adaptation of new settlers in the Arctic territories proceeds in three stages. The duration of the shift period must have a physiological and hygienic justification and correspond to the duration of the period of stable working capacity. In high latitudes, it is especially important to develop diets and food products for various groups of the population and shift workers in relation to the specifics of their work in the Arctic regions

Conclusions. Among the main environmental and hygienic problems of the Arctic territories, where intense industrial activity is carried out, it is necessary to highlight the tasks of scientific substantiation of rational modes of work and rest of workers in rotational forms of work. Also it’s necessary to define the quantitative and qualitative composition of food rations recommended for nutrition of various groups of the population, in relation to the specifics of production factors.
Developing Interactive E-Book for Early Reading-Writing Stage at Class I Elementary School
Authors: Janawati Desak Putu Anom , Pradnyana Putu Beny , Darmayanti N.W.S.
Published: January 2022

The interactive e-book was developed to improve early reading and writing skills as log of pandemic Covid-19. The type of this research is R&D with the product of this research is the E-Book Interactive reading and writing starters. The questionnaire instrument was used as a research data collection technique. The results of the product validity test show the following results. First, the appearance of the interactive E- book product has an attractive appearance, second, the selection of an attractive typeface and the presentation of animated text in the form of a video embedded in the interactive E-book. Third, to get students involved in learning activities it is necessary to have interaction in learning, interesting content and presentations combbined with learning videos to explain the material interactively
Haloterrigena Gelatinilytica sp. nov., A New Extremely Halophilic Archaeon Isolated From Salt-Lake
Authors: Liu Bing-Bing , Salam Nimaichand , Rao Manik Prabhu Narsing , Cheng Shuang , Xie Yuan-Guo , Wang Lai-You , Zhang Yuan-Ru , Yu Xin-Yuan , Guo Shu-Xian , Li Wen-Jun
Published: January 2022

Two extremely halophilic strains, designated SYSU A558-1T and SYSU A121-1, were isolated from a saline sediment sample collected from Aiding salt lake, China. Cells of strains SYSU A558-1T and SYSU A121-1 were Gram-stain-negative, coccoid, and non-motile. The isolates were aerobic and grew at NaCl concentration of 10-30% (optimum, 20-22%), at 20-55ºC (optimum, 37-42ºC) and at pH 6.5-8.5 (optimum, 7.0-8.0). Cells lysed in distilled water. Major polar lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, disulphated diglycosyl diether-1 and one unidentified glycolipid. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the two strains SYSU A558-1T and SYSU A121-1 were closely related to the membranes of the genus Haloterrigena. Phylogenetic trees based on strains SYSU A558-1T and SYSU A121-1 16S rRNA gene sequence, rpoB' gene sequence and concatenation of 87 protein markers demonstrated a robust clade with Haloterrigena turkmenica, Haloterrigena salifodinae and Haloterrigena salina. The genomic DNA G+C contents of strains SYSU A558-1T and SYSU A121-1 were 65.8 and 65.0%, respectively. Phenotypic, chemotaxonomic characteristics and phylogenetic properties suggested that the two strains SYSU A558-1T and SYSU A121-1 represent a novel species of the genus Haloterrigena, for which the name Haloterrigena gelatinilytica sp. nov. is proposed. The type strain is SYSU A558-1T (= KCTC 4259T = CGMCC 1.15953T).
A Gastrointestinal Metagenomic Study On Mudskippers Supports Their Terrestrial Adaptation
Authors: Dong Bo , Liu Jing , Chen Bing , Huang Yuqi , Ai Peng , You Xinxin , Shi Qiong , Huang Wen
Published: January 2022

-Purpose: The adaptability of blue-spotted mudskipper (Boleophthalmus Periophthalmodon; BP) and giant-fin mudskipper (Periophthalmus magnuspinnatus; PM), has been previously reported at the genome level to explain their amphibious life. However, the roles of GI microbiota in their adaptation to the terrestrial life are worth exploring. -Methods: In this study, we mainly utilized metagenomic data from these two representative mudskippers and typical aquicolous fish species to obtain microbial composition, diversity, abundance and potential functions of GI microbiota for comparisons between amphibious and aquicolous fishes. Meanwhile, we summarized the GI microbiota results of representative seawater fishes, freshwater fishes, amphibians, and terrestrial animals by literature mining for comparing those of the mudskippers. -Result: Interestingly the content for each dominant phylum was strikingly different among BP, PM and aquicolous fishes. We also observed that the profile of GI microbiota in mudskippers owned the typical bacterial families for the terrestrial animals, (freshwater and seawater) fishes, and amphibians at the same time, which is consistent with their life style of water-to-land and freshwater to seawater transition. More interestingly, certain bacteria strains like S24-7, previously thought to be specific in terrestrial animals, were also identified in both BP and PM. -Conclusion: The various composite and diversity of mudskipper GI microflora are therefore considered to conduce to their terrestrial adaptation in these amphibious fishes.
SNHG12 Regulated by KMT2B Participates in the Pathogenesis of Renal Cell Carcinoma via E2F1/CEP55
Authors: Feng Jia-fu , Wang Jun , Xie Gang , Wang Yao-dong , Li Xiao-han , Yang Wen-yu , Yang Yu-wei , Zhang Bin
Published: January 2022

Objective: This study is to investigate the regulation of long non-coding RNA (lncRNA) SNHG12 promoter methylation modification by KMT2B and the mechanism of SNHG12 in the development of renal cell carcinoma (RCC) involving E2F1/CEP55 axis.Methods: TCGA and GEO databases were used to predict the involvement of SNHG12 in RCC. Knockdown of SNHG12/E2F1/CEP55 was performed. Next, SNHG12 expression and other mRNAs were analyzed by RT-qPCR. Subsequently, CCK-8 was used to detect cell proliferation. Wound healing test and Transwell assay was used to detect cell migration and invasion, respectively. The vascularization of HUVEC was explored by in vitro pseudotubule formation. CHIP was used to detect H3K4me3 in SNHG12 promoter region. The binding of E2F1 and CEP55 promoter region was analyzed with CHIP and dual luciferase reporter assay. RIP was used to detect the binding of SNHG12 and E2F1. Finally, the effect of SNHG12 on the tumor formation and angiogenesis of RCC was assessed in nude mouse xenograft model.Results: Bioinformatics analysis showed that SNHG12 was highly expressed in RCC tissues and cells, and it was related to the poor prognosis of RCC patients. SNHG12 knockdown significantly inhibited RCC cell proliferation, migration, and invasion and HUVEC angiogenesis. KMT2B up-regulated SNHG12 through modifying H3K4me3 in its promoter region. In addition, SNHG12 promoted CEP55 expression by recruiting the transcription factor E2F1. Knockdown of SNHG12 blocked E2F1 recruitment, thereby down-regulating the expression of CEP55, and inhibited tumor formation and angiogenesis of RCC cells in nude mice.Conclusion: KMT2B up-regulates SNHG12 via the modification of H3K4me3 in its promoter region. SNHG12 recruits E2F1 to promote the expression of CEP55, and ultimately promotes RCC cell proliferation, migration, and invasion and HUVEC angiogenesis.
Accessory and Cavitated Uterine Mass (ACUM) in an 18-Year-Old Woman: A Case Report and Literature Review
Authors: He HaiJing , Liang XuZhi , Fan Jiangtao
Published: January 2022

Background

Accessory and cavitated uterine mass (ACUM) is a rare uterine anomaly newly recognized as a form of developmental Mullerian anomaly, which represents a non-communicating uterus-like mass within an otherwise normal uterus. It is a benign gynecological disease associated with severe dysmenorrhea and chronic pelvic pain, which is most common in young nullipara women, and sometimes develops in parous women. Clinical manifestations combined with imaging examinations including ultrasonography (USG), magnetic resonance imaging (MRI), and hysterosalpingography (HSG) are the means to establish a correct diagnosis. Medical therapy is only marginally effective, but laparoscopic surgery for complete mass excision is a feasible technique to relieve patient’s symptoms. Our article is aimed to report a case of ACUM in an 18-year-old woman and summarize the diagnostic criteria of ACUM.
Case presentation

: An 18-year-old woman was admitted for severe pain in the right lower abdomen during menstruation, which lasted more than 1 year. The patient was misdiagnosed with focal adenomyosis at our hospital on March 4, 2021. After 4 months, she was diagnosed with ACUM. Once diagnosis as focal adenomyosis, nonsteroidal anti-inflammatory drugs (NSAIDs) and gestrinone were administered to the patient. Following the diagnosis of ACUM, she received laparoscopic surgery. Our follow-up indicated that the symptom was significantly relief without drug therapy after sixty days postoperatively.
Conclusions

Clinical manifestations and imaging examinations are used to establish the diagnosis of ACUM. Medical therapy is only marginally effective, but laparoscopic surgery for complete mass excision is a feasible technique to solve the pain symptom. The prevalence and pathogenesis of ACUM and its reproductive outcomes on patients remain unclear, which calls for more and deeper research to study.
Pilot-Scale Feasibility Study for the Stabilization of Coal Tailings via Microbially-Induced Calcite Precipitation
Authors: Rodin Sarah , Champagne Pascale , Mann Vanessa
Published: January 2022

Sustainable long-term solutions to managing tailings storage facilities (TSFs) are integral for mines to operate in a safe and environmentally responsible manner. The long-term storage of subaqueous tailings can pose significant safety, environmental, and economic risks, therefore alternative containment strategies for maintaining geochemical stability of reactive materials must be explored. In this study, the physical and geochemical stabilization of coal tailings using microbial induced calcite precipitation (MICP) was evaluated at a laboratory pilot scale. Three application techniques simulated commonly used agricultural approaches and equipment that could be deployed for field-scale treatment: spraying on treatment solutions with irrigation sprinklers, mixing tailings and treatment solutions with a rototiller, and distributing treatment solutions via shallow trenches using an excavator ripper. Test cells containing 1.0 x 1.0 x 0.5m of tailings were treated with ureolytic bacteria (Sporosarcina pasteurii) and cementation solutions composed of urea and calcium chloride for 28 days. Penetrometer tests were performed following incubation to evaluate the extent of cementation. The spray-on application method showed the greatest strength improvement, with in an increase in surface strength of more than 50% for the 28-day testing period. The distribution of treatment solution using trenches was found to be less effective and resulted in greater variability in particle size distribution of treated tailings and would not be recommended for use in the field. The use of rototilling equipment provided a homogenous distribution of treatment solution, however, the disruption to the tailings material was less effective for facilitating effective cementation. Bacterial plate counts of soil samples indicated that S. pasteurii cultures remained viable in a tailings environment for 28 days at 18˚C and near-neutral pH. The treatment was also found to stabilize the pH of tailings porewater sampled over the 28-day incubation period, suggesting the potential for the treatment to provide short-term geochemical stability under unsaturated conditions.
Prognostic and Predictive Value of the Pretreatment Albumin-bilirubin Score for Short and Long-term Survival in Patients With Non-Hodgkin Lymphoma-associated Hemophagocytic Lymphohistiocytosis With Hepatic Injuries
Authors: Yin Guangli , Cheng Wanying , Shu Yongqian , Qiu Hongxia , Duan Limin
Published: January 2022

Purpose: This study aimed to assess the prognostic value of pretreatment albumin-bilirubin (ALBI) on short-term mortality (30 days) and long-term (≥ 1 year) survivalThe aim of this study was to construct a prognosis model of non-Hodgkin lymphoma-associated secondary hemophagocytic lymphohistiocytosis (NHL-sHLH) patients with hepatic injuries by the combination of ALBI score and clinical parameters.Material and methods: This retrospective study included 168 NHL-sHLH patients with hepatic injuries between February 1, 2014, and February 1, 2020. Multivariable logistic/Cox models and restricted cubic spline models were conducted to evaluate the relationships between the ALBI score and short- and long-term survival. The predictive performance of the ALBI score was assessed and compared using time-dependent receiver operating characteristic (ROC) analysis.Results: Among 168 adult NHL-sHLH patients, 82 (48.8%) patients died within 30 days after admission, and 144 (85.7%) patients died during the follow-up period. Multivariate logistic regression indicated that ALBI grade could be an independent risk factor for predicting the prognosis of patients with 30-day mortality and overall survival (odds ratios [OR]30 days 5.37, 95% confidence interval 2.41-12.64, P < 0.001; hazard ratios [HR]OS 1.52, 95% confidence interval 1.06-2.18, P = 0.023), respectively. The restricted cubic spline curve displayed a linear and positive relationship between the ALBI score and risk of mortality (P for nonlinearity =0.503). Furthermore, receiver operating characteristic (ROC) curve analysis showed that the area under the curve (AUC) for predicting mortality by integrative analysis of the ALBI score and ferritin was significantly improved compared to the ALBI score (AUC 30 days: 0.820 vs 0.693, P = 0.001; AUC1 year: 0.754 vs 0.681, P = 0.043) or ferritin (AUC30 days: 0.820 vs 0.724, P = 0.005; AUC1 year: 0.754 vs 0.658, P = 0.031) alone.Conclusions: These results suggest that the ALBI score could be a useful indicator of 30-day mortality and overall survival (≥1 year) for NHL-sHLH patients with hepatic injuries, and the combination analysis of the ALBI score and ferritin provides incremental prognostic value for clinical use.
A Mitophagy-Related Gene Signature Associated With Prognosis and Immune Microenvironment in Colorectal Cancer
Authors: Zhang Cong , Zeng Cailing , Xiong Shaoquan , Zhao Zewei , Wu Guoyu
Published: January 2022

Background: Colorectal cancer (CRC) is a heterogeneous disease and one of the most common malignancies in the world. Previous studies have found that mitophagy plays an important role in the progression of colorectal cancer. This study is aimed to investigate the relationship between mitophagy-related genes and the prognosis of patients with CRC.Methods: Gene expression profiles and clinical information of CRC patients were obtained from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Univariate Cox regression and the least absolute shrinkage and selection operator (LASSO) regression analysis were used to establish the prognostic signature composed of mitophagy related genes. Kaplan-Meier curve and receiver operating characteristic (ROC) curve were used to analyze patient survival and verify the predictive accuracy of the signature, respectively. Construction of a nomogram prognostic prediction model was based on risk scores and clinicopathological parameters. Using the Genomics of Drug Sensitivity in Cancer (GDSC) database and Tumor Immune Dysfunction and Exclusion (TIDE) algorithm to estimate the sensitivity of chemotherapy, targeted therapy and immunotherapy. Results: A total of 44 mitophagy-driven genes connected with CRC survival were identified, and prognostic signature was established based on the expression of 10 of them (AMBRA1, ATG14, MAP1LC3A, MAP1LC3B, OPTN, VDAC1, ATG5, CSNK2A2, MFN1, TOMM22). Patients were divided into high-risk and low-risk groups based on the median risk score, and the survival of patients in the high-risk group was significantly shorter than that of the low-risk group among the TCGA cohort (median OS 67.3 months vs not reached, p=0.00059) and two independent cohorts from GEO (median OS in GSE17536: 54.0 months vs not reached, p=0.0082; in GSE245: 7.7 months vs not reached, p=0.025). ROC curve showed that the area under the curves (AUC) of 1-, 3- and 5-year survival were 0.66, 0.66 and 0.64, respectively. Multivariate Cox regression analysis confirmed the independent prognostic value of the signature. Then we constructed a nomogram combining the risk score, age and M stage, which had a concordance index of survival prediction of 0.77 (95% CI=0.71-0.83) and more robust predictive sensitivity and specificity. Results showed that CD8+ T cells, regulatory T cells and activated NK cells were significantly more abundant in the high-risk group. Furthermore, patients in the high-risk group were more sensitive to potential targeted therapies, including Motesanib, ATRA, Olaparib, Selumetinib, AZD8055 and immunotherapy. Conclusion: In conclusion, we constructed and validated a novel mitophagy related gene signature that can be used as an independent prognostic biomarker for CRC, and may lead to better stratification and selection of precise treatment for CRC patients.
Scaling of cellular proteome with ploidy
Authors: Yahya Galal , Menges Paul , Ngandiri Devi , Schulz Daniel , Wallek Andreas , Kulak Nils , Mann Matthias , Cramer Patrick , Savage Van , Raeschle Markus , Storchova Zuzana
Published: January 2022

Ploidy changes are frequent in nature and contribute to evolution, functional specialization and tumorigenesis. Analysis of model organisms of different ploidies revealed that increased ploidy leads to an increase in cell and nuclear volume, reduced proliferation, metabolic changes, lower fitness, and increased genomic instability, but the underlying mechanisms remain poorly understood. To investigate how the gene expression changes with cellular ploidy, we analyzed isogenic series of budding yeasts from 1N to 4N. We show that mRNA and protein abundance scales allometrically with ploidy, with tetraploid cells showing only threefold increase in proteins compared to haploids. This ploidy-specific scaling occurs via decreased rRNA and ribosomal protein abundance and reduced translation. We demonstrate that the Tor1 activity is reduced with increasing ploidy, which leads to rRNA gene repression via a novel Tor1-Sch9-Tup1 signaling pathway. mTORC1 and S6K activity are also reduced in human tetraploid cells and the concomitant increase of the Tup1 homolog Tle1 downregulates the rDNA transcription. Our results revealed a novel conserved mTORC1-S6K-Tup1/Tle1 pathway that ensures proteome remodeling in response to increased ploidy.
Fourth Year Intellectual Disability Student Nurses’ Journey and Future Work Intention: A Qualitative Study.
Authors: Doody Owen , Meskell Pauline , Noonan Maria , Murphy-Tighe Sylvia , Kingston Liz
Published: January 2022

BackgroundThe aim of this qualitative study is to explore the views and experiences of final year BSc intellectual disability nursing students’ journey, future work plans and examine factors influencing their migration intentions following graduation.MethodsA qualitative component of a mixed methods study where focus group interviews were conducted with final year BSc intellectual disability nursing students (n-=10). A topic guide was utilised, and participants were interviewed about their programme, future work plans and migration intentions. An inductive approach was utilised, and data were analysed using a pre-existing framework for initial coding and thematic development. Duffy’s conceptual model of identity transformation provided a structure to analyse the data and map themes onto the conceptual framework.ResultsThe findings were mapped onto the five stages of Duffy’s (2013) conceptual model of identity transformation: Pre-Entry; Reaffirming; Surmounting; Stabilising and Actualising. Findings indicate that further work is required to promote intellectual disability nursing and address professional esteem issues, support for education and professional development, such as providing career guidance opportunities prior to course completion, development of clinical skills within their education programme and support for the professional development of new graduates. Participants identified uncertainty about career opportunities and saw scope for future professional developments opportunities particularly in community-based work.ConclusionThis study has identified that final year intellectual disability nursing students are uncertain about career options and opportunities for intellectual disability nurses in other countries. There is an urgent need for the intellectual disability nursing profession to articulate their practice and advocate for their role and contribution to the care of people with intellectual disability. This study identified a clear need for direction and information regarding intellectual disability nursing roles and career opportunities.
Penicillium Arizonense as a Novel Producer Strain for Mycophenolic Acid and Expression Analysis of Biosynthesizing Gene Clusters
Authors: Ammar Hala , Ezzat Saeid , Elshourbagi Ebrahim , Elshahat Hind
Published: January 2022

A novel potent mycophenolic acid (MPA) producer strain of the genus Penicillium was isolated from refrigerated Mozzarella cheese and identified as P. arizonenseHEWt1. The molecular mechanism of MPA production by this new isolate was our main target. To achieve this objective, we first isolated three MPA overproducer mutants by exposing the wild type to different doses of gamma-rays, and the fermentation conditions for the highest production of MPA by both the wild type and mutants were optimized. Then, orthologs of MPA gene clusters in P. brevicompactum were cloned and predicted from the genome of P. arizonense. Sequencing and bioinformatic analysis proved the presence of a cluster containing five putative genes in the P. arizonense HEWt1 genome ortholog to the MPA cluster, mpaA, mpaC, mpaF, mpaG, and mpaH. All predicted genes displayed 96-97% similarity with the related hypothetical protein of P. arizonense. The genes, mpaG, mpaC, and mpaF. represented 69%, 82%, 84%, respectively, similarity with their orthologous genes in P. brevicompactum, whereas mpaG and mpaA represented 75% and 79%, respectively, similarity to their orthologous genes in P. roqueforti. Gene expression analysis through quantitative rPCR indicated an increase in the transcription value of all annotated genes in the three mutants over the wild type. A highly significant increase in the gene expression of mpaC, mpaF, and mpaH was observed, with 8.4561±1.02, 5.6569±0.87, and 4.6268±0.18-fold increases, respectively, in P. arizonense-MT1 compared with wild-type. These results confirmed the potential participation of these genes in MPA biosynthesis and are the first report regarding the molecular mechanism of MPA production by P. arizonense.
Plinabulin and Pegfilgrastim in Combination for the Prevention of Chemotherapy-Induced Neutropenia in Patients with Breast Cancer (PROTECTIVE-2): A Randomized Trial
Authors: Blayney Douglas , Zhang Qingyuan , Mohanlal Ramon , Du Lihua , Ette Ene , Ogenstad Stephan , Adamchuk Hryhoriy , Sun Tao , Li Hongsheng , Feng Jifen , Huang Lan , Shi Yuan-kai
Published: January 2022

PurposePlinabulin is a non-granulocyte colony-stimulating factor (G-CSF) novel small molecule with both anticancer and myeloprotective effects. Single-agent plinabulin is myeloprotective in the first week of the chemotherapy cycle, and pegfilgrastim in the second week. We assessed the efficacy and safety of the combination of plinabulin and pegfilgrastim for the prevention of chemotherapy-induced neutropenia (CIN) following chemotherapy.MethodsThis randomized, open-label, Phase 2 trial enrolled patients with breast cancer. All received docetaxel 75 mg/m2, doxorubicin 50 mg/m2, and cyclophosphamide 500 mg/m2 on Day 1. In the combined therapy cohort, patients received plinabulin 20 mg/m2 on Day 1 and 1.5, 3, or 6 mg pegfilgrastim on Day 2. The primary objective was to establish the recommended Phase 3 dose (RP3D). Secondary endpoints included absolute neutrophil count (ANC) nadir, relative dose intensity (RDI), and incidence of adverse events including neutropenia and bone pain.ResultsIn total, 115 patients were randomized and evaluated. The combination therapy at the RP3D (plinabulin 20 mg/m2 and pegfilgrastim 6 mg) was well-tolerated and had superior CIN prevention in terms of Grade 4 and Grade 3/4 neutropenia frequency, absolute neutrophil count (ANC) nadir, higher relative dose intensity (RDI), less bone pain, and less toxicity burden when compared with pegfilgrastim 6 mg alone.ConclusionPlinabulin combined with pegfilgrastim at the RP3D (plinabulin 20 mg/m2 Day 1 and pegfilgrastim 6 mg Day 2) had more favorable efficacy, safety, and tolerability profiles and lower bone pain incidence than did pegfilgrastim alone.Trial information Clinical Trial Registration: ClinicalTrials.gov NCT04227990Date registered: January 14, 2020 Retrospectively registered
Köppen–Geiger Climate Clasification in the Pannonian basin According to SSP5-8.5 Scenario
Authors: Ruman Albert , Ruman Anna
Published: January 2022

The Köppen–Geiger climate classification is used to determine climate types in region of Pannonian Basin with data from the sixth phase of the Coupled Model Intercomparison Project. The study covers a period from years 2021 until 2100, and it shows how certain climate types are changing in percentage in thirty-year averages for six periods. In the period 1960-1990 years of the last century, the dominant climate type was warm summer humid continental climate (Dfb) with 98% presences in the region. The results show that the change of this climate type to the humid subtropical climate type (Cfa) began in the first half of the 21st century. The complete dominance of humid subtropical climate type in the most areas of the Pannonian Basin characterized the second half of the 21st century. Also, results show creation of a warm summer Mediterranean climate type (Csa), which according to certain simulations, is present from 10% to 30% on average in the region. In the central part of the region, a cold desert climate type (Bsk) was formed with approximately 6% presences in the region. This creation of climate types in some parts of the region shows that in the second half of 21st century drier and a warmer climate is expected compared to the last century.
Normal product form of two-mode Wigner operator
Authors: He Rui , Liu Xiangyuan , Wei Xiangfei , Wu Congbing
Published: January 2022

In the context of normal product, we use the method of the integration within an ordered product (IWOP) of operators to derive three representations of the two-mode Wigner operator: SU (2) symmetric description, SU (1, 1) symmetric description and polar coordinate form. We find that two-mode Wigner operator has multiple potential degrees of freedom. As the physical meaning of the selected integral variable changes, Wigner operator shows different symmetries. In particular, in the case of polar coordinates, we reveal the natural connection between the two-mode Wigner operator and the entangled state representation.
Designing an Optimal Model of Blood Logistics Management with the Possibility of Return in the Three-Level Blood Transfusion Network
Authors: Ahmadimanesh monireh , Pooya Alireza , Safabakhsh Hamidreza , Sadeghi Sedigheh
Published: January 2022

Inventory managers in the blood supply chain always seek timely and proper response to their customers, which is essential because of the perishability and uncertainty of blood demand and the direct relationship of its presence or non-presence with human life. On the other hand, timely and regular delivery of blood to consumers is vital, as the weakness in delivery and transportation policies results in increased shortages, returns, blood loss and significant decrease in the quality of blood required by patients. Given the significance of this for the blood transfusion network, the paper tried to design a comprehensive and integrated optimal model of blood transfusion network logistics management by blood group to reduce the cost of losses, returns and blood shortages. This model is divided into two parts: Inventory management and routing. A combination of simulation techniques and neural network with several recurrent layers was used to evaluate the optimal inventory management and a multi-objective planning model was designed to determine the delivery and distribution of blood to consumers. The model designed was implemented in Khorasan Razavi Blood Transfusion Network with a main base, six central bases and 54 hospitals. Solving the model led to estimating the f consumer demand, the optimal value of target inventory and re-ordering point of central bases and hospitals, and blood distribution from the supplier to its consumers that decreased the units of blood returned to bases, increased inventory availability, and reduced costs significantly.
Measurement and Mapping of Maternal Health Service Coverage Through a Novel Composite Index: A Sub-National Level Analysis in India
Authors: Kiran Tanvi , Junaid KP , Rajagopal Vineeth , Gupta Madhu , Sharma Divya
Published: January 2022

Background: Expansion of maternal health service coverage is crucial for the survival and wellbeing of both mother and child. To date, limited literature exists on the measurement of maternal health service coverage at the sub-national level in India. The prime objectives of the study were to comprehensively measure the maternal health service coverage by generating a composite index; map India by categorizing it into low, medium and high zones and examine its incremental changes over time. Methods: Utilising a nationally representative time series data of 15 key indicators spread across three domains of antenatal care, intranatal care and postnatal care, we constructed a novel ‘Maternal Health Service Coverage Index’ (MHSI) for 29 states and 5 union territories of India for the base (2017-18) and reference (2019-20) years. Following a rigorous procedure, MHSI scores were generated using both arithmetic mean and geometric mean approach. We categorized India into low, medium and high maternal health coverage zones and further generated geospatial maps to examine the extent and transition of maternal health service coverage from base to reference year.Results: India registered the highest mean percentage coverage (93.66%) for ‘institutional delivery’ and lowest for ‘treatment for obstetric complications’ (9.25%) among all the indicators. Depending on the usage of arithmetic mean and geometric mean approach, the maternal health service coverage index score for India exhibited marginal incremental change (between 0.015- 0.02 index points) in the reference year. West zone exhibited an upward transition in the coverage of maternal health service indicators, while none of the zones recorded a downward movement. States of Mizoram (east zone), Puducherry (south zone) showed a downward transition. Union territories of Dadra & Nagar Haveli (west zone) and Chandigarh (north zone) along with the states of Maharashtra (west zone), Assam, as well as Jharkhand (both from the east & north east zone), showed upward transition.Conclusion: Overall, maternal health service coverage is increasing across India. Our study offers a novel summary measure to comprehensively quantify the coverage of maternal health service, which can momentously help India to identify lagged indicators and low performing regions, thereby warranting the targeted interventions and concentrated programmatic efforts to bolster the maternal health service coverage at the sub-national level.
Evaluation of Psychopathological Conditions, Corona Anxiety, Death Anxiety and Suicide Risk in Hemodialysis Patients During the COVID-19 Pandemic
Authors: Korkut Süleyman
Published: January 2022

Background

Hemodialysis (HD) patients have serious psychopathological symptoms due to COVID-19 pandemic. Therefore, this study aimed to identify the psychopathological conditions among HD patients during the current pandemic and also to provide a theoretical basis for the implementation of effective preventions and psychological interventions.
Methods

This cross-sectional study was conducted with 114 HD patients who were undergoing treatment in the Dialysis Centers of two state hospitals between July and October 2021. Brief symptom inventory, Death anxiety scale, Suicide probability scale and Corona anxiety scale were applied to the participants for the assessment.
Results

In the study, it was determined that HD patients had high levels of psychopathological symptoms. The mean total score was found to be 26.47 ± 20.2 in TDAS, 0.71 ± 0.51 in GSI, 3.31 ± 2.86 in CAS and 65.86 ± 9.72 in SPS. Of the participants 18.4% had high death anxiety levels and 30.7% had corona anxiety. Suicide risk was higher in HD patients. In correlation analysis; psychological distress positively correlated with suicide probability, corona anxiety and death anxiety. On the other hand, there was a positive correlation between corona anxiety and death anxiety.
Conclusions

As a result, it was concluded that HD patients experienced various mental health problems during the COVID-19 pandemic. Psychosocial support and interventions need to be planned by the healthcare system and healthcare providers to help HD patients in managing their disease and related mental health conditions.
A comparative and diachronic analysis of film title translations and appellative effect transfer into Croatian and German
Authors: Gabrić Petar , Brajković Iva , Licchetta Letizia , Ključarić Dorotea Kelčec , Bezuh Juraj
Published: January 2022

Studies of film title translations remain scant to this day. The existing studies mainly focus on investigating the sources of difficulties during the translation process. Although the studies employ different analytical approaches, the conclusion in almost all investigations is that the decisive objective during the translation process is the transfer or production of the appellative effect. This study investigates which strategies are employed during translation into Croatian and German and why, as well as possible diachronic changes in the choice of translation strategies. We created a corpus of 935 film titles from 1923 to 2017 and their translations into Croatian and German, which we first classified as either direct translations, free translations, transcreations or transcriptions, and finally we quantitatively and qualitatively analysed the data. Our results show significant differences between the two subcorpora in the choice of translation strategies and motivation, as well as in the patterns of diachronic change. Furthermore, correlations with specific cultural-historical processes are observed.
Loss of HAS2 Confers Acquired Antiestrogen Resistance By Upregulating Ezrin Expression In ER-Positive Breast Cancer
Authors: Sun Xiaodan , Tang Fen , Liu Yiwen , He Yiqing , Du Yan , Zhang Guoliang , Gao Feng , Yang Cuixia
Published: January 2022

Background: Resistance to endocrine therapy is a major challenge for estrogen receptor-positive (ER+) breast cancer patients, but the underlying mechanisms remain unclear. Methods: Loss of hyaluronan synthase 2 (Has2) in adaptive resistant cells to tamoxifen and fulvestrant was observed by immunblotting assay. CRISPR/Cas9 technology was used to knock out Has2 in MCF7 cells to verify the effect of Has2 on the expression of ER and Ezrin and Akt and MAPK/ERK signaling routes. We utilized an Ezrin small-interfering RNA and Ezrin inhibitor to inhibit Ezrin expression for evaluating Has2 and ERα expression and the Akt/MAPK signaling cascade upon tamoxifen or fulvestrant treatment.Results: In this work, we showed that a Has2-loss state was acquired from adaptive resistance to tamoxifen and fulvestrant in luminal BrCas. Notably, the adapted loss of Has2 induced acquired resistance to antiestrogens in estrogen receptor (ER)-positive breast cancer cells through up-regulating the expression of Ezrin. Furthermore, we found that the loss of Has2 promoted while the consequent increase of Ezrin inhibited ERα expression/activity through the Akt and MAPK/ERK signaling routes, indicating an opposite effect on ERα expression during the development of antiestrogens-resistance. Inhibition of Ezrin reversed Has2 and ERα expression and the Akt/MAPK signaling cascade upon tamoxifen or fulvestrant, suggesting a Has2-Ezrin-ER negative-feedback loop in governing cellular sensitivity to tamoxifen or fulvestrant in luminal-like breast cancer cells. Finally, Knockdown or inhibition of Ezrin restored sensitivity to antiestrogens, implying that Ezrin could be a potential therapeutic target to tackle endocrine resistance. Conclusions: Taken together, our findings provide a direct relationship between ERα and Has2 implicated in resistance to endocrine therapy and a new insight into how ERα-signaling is regulated upon antiestrogens treatment, suggesting a novel therapeutic target for ER-positive breast cancer.