bluuebunny/crossref_metadata_2025 huggingface dataset:
Insights into the Global Transcriptome Response of Lentinula edodes Mycelia during Aging
Authors: Gao Qi , Fan Yangyang , Wei Sai , Song Shuang , Guo Yuan , Wang Shouxian , Liu Yu , Yan Dong
Published: March 2023

The spawn of Lentinula edodes and other basidiomycete fungi tend to age with long-term culture. This causes heavy yield losses if aging spawn is used for propagation. In this study, we cultivated dikaryotic L. edodes mycelia in plates for 60 days to produce intrinsic aging phenotypes. We found that intracellular reactive oxygen species levels increased in contrast to mitochondrial depolarization and also observed greater DNA fragmentation with longer culture time. Transcriptome analysis of mycelia at different growth stages revealed pronounced expression differences between short- and long-term cultures. In particular, “phenylalanine, tyrosine, and tryptophan biosynthesis”, “mitophagy and autophagy”, “MAPK signaling pathway”, and “ABC transporter” were among the enriched terms in the mycelial aging process. Weighted correlation network analysis identified LeAtg8, LeHog1, LePbs2, and LemTOR as key genes during aging. Western blotting confirmed that LeATG8 and phosphorylated LeHOG1 protein levels were significantly upregulated in aging mycelia. Our combined analytical approach provides insights into the mechanisms that regulate mycelial aging, indicating that autophagy/mitophagy plays a major role in counteracting the effects of age on mycelial growth development.
Clinical Manifestations of Human Exposure to Fungi
Authors: Oliveira Manuela , Oliveira Diana , Lisboa Carmen , Boechat José , Delgado Luís
Published: March 2023

Biological particles, along with inorganic gaseous and particulate pollutants, constitute an ever-present component of the atmosphere and surfaces. Among these particles are fungal species colonizing almost all ecosystems, including the human body. Although inoffensive to most people, fungi can be responsible for several health problems, such as allergic fungal diseases and fungal infections. Worldwide fungal disease incidence is increasing, with new emerging fungal diseases appearing yearly. Reasons for this increase are the expansion of life expectancy, the number of immunocompromised patients (immunosuppressive treatments for transplantation, autoimmune diseases, and immunodeficiency diseases), the number of uncontrolled underlying conditions (e.g., diabetes mellitus), and the misusage of medication (e.g., corticosteroids and broad-spectrum antibiotics). Managing fungal diseases is challenging; only four classes of antifungal drugs are available, resistance to these drugs is increasing, and no vaccines have been approved. The present work reviews the implications of fungal particles in human health from allergic diseases (i.e., allergic bronchopulmonary aspergillosis, severe asthma with fungal sensitization, thunderstorm asthma, allergic fungal rhinosinusitis, and occupational lung diseases) to infections (i.e., superficial, subcutaneous, and systemic infections). Topics such as the etiological agent, risk factors, clinical manifestations, diagnosis, and treatment will be revised to improve the knowledge of this growing health concern.
Using Risk System Theory to Explore Farmers’ Intentions towards Rural Homestead Transfer: Empirical Evidence from Anhui, China
Authors: Guan Gexin , Zhao Wei
Published: March 2023

In China, rural homesteads are strategic elements for coordinating the people and land resources relationship between urban and rural regions and are powerful means for increasing the property income of farmers. The rural homestead transfer issue has always concerned policymakers. In this paper, the risk system theory framework is employed to identify risk source (risk perception), risk control (the control measures and institutions), and risk receptor (farmers’ characteristics) to explore farmers’ intentions towards rural homestead transfer from the perspective of first- and second-order observation. The results demonstrate that farmers’ intentions are significantly affected by risk source from first-order observation, and risk control and risk receptor from second-order observation. The heterogeneity of outcomes is examined by grouping of ages and regions of sampled farmers. It is suggested that the risk perception needs of farmers should be respected, land tenure of rural homesteads should be tightly protected, and farmers’ characteristics should be of great concern. Moreover, more attention should be paid to idle homesteads, and full advantage taken of rural resources to develop featured rural industries.
The Association between Carbon Emission and Urban Spatial Form—A Study of Zhuhai, China
Authors: Zhang Yiheng , Zhang Shengyong , Gong Yabo
Published: March 2023

Research on carbon emission is an important basis for solving global climate problems, and it is also one of the ways to quantitatively assess the impact of human activities. Cities are one of the main bearing spaces of human activities, and reasonable urban form is conducive to reducing energy consumption in human activities. This paper takes 50 clusters within Zhuhai, China, as the research object, quantifies the landscape spatial form index and social spatial form index of each cluster and establishes the index set of urban spatial form, so as to analyze the influence of different urban spatial form index on carbon emission. The main conclusions are as follows: (1) From the spatial distribution of each index, the urban cluster size and residential building area of each cluster in Zhuhai are generally large, and the distribution is basically consistent with that of densely populated areas. The urban clusters with high dominance are mainly located in the main urban area of Xiangzhou District, and the urban compactness, dispersion and industrial building area are generally high in the west and low in the east. (2) The size of urban clusters, industrial building area and residential building area have a strong promoting effect on carbon emission, while the compactness, dispersion and dominance of urban clusters have a strong inhibiting effect on carbon emission. (3) Based on the above conclusions, the low-carbon urban spatial form optimization strategy should be proposed from three aspects: urban development boundary control, promoting industrial structure transformation and compact urban development.
Shedding Lights on the Extracellular Vesicles as Functional Mediator and Therapeutic Decoy for COVID-19
Authors: Thakur Abhimanyu
Published: March 2023

COVID-19 is an infectious disease caused by the novel coronavirus (SARS-CoV-2) that first appeared in late 2019 and has since spread across the world. It is characterized by symptoms such as fever, cough, and shortness of breath and can lead to death in severe cases. To help contain the virus, measures such as social distancing, handwashing, and other public health measures have been implemented. Vaccine and drug candidates, such as those developed by Pfizer/BioNTech, AstraZeneca, Moderna, Novavax, and Johnson & Johnson, have been developed and are being distributed worldwide. Clinical trials for drug treatments such as remdesivir, dexamethasone, and monoclonal antibodies are underway and have shown promising results. Recently, exosomes have gained attention as a possible mediator of the COVID-19 infection. Exosomes, small vesicles with a size of around 30–200 nm, released from cells, contain viral particles and other molecules that can activate the immune system and/or facilitate viral entry into target cells. Apparently, the role of exosomes in eliciting various immune responses and causing tissue injury in COVID-19 pathogenesis has been discussed. In addition, the potential of exosomes as theranostic and therapeutic agents for the treatment of COVID-19 has been elaborated.
Toward Greening City Logistics: A Systematic Review on Corporate Governance and Social Responsibility in Managing Urban Distribution Centers
Authors: Mansouri Bahareh , Sahu Subhasmita , Ülkü M. Ali
Published: March 2023

Background: The ramifications of climate change are rampant: All stakeholders must act effectively and swiftly. Unsustainable and increased urbanization adds additional strain on combatting environmental degradation. Since the last decade, urban distribution centers (UDCs) have emerged in response to the steep rise in urban freight transportation and its negative impact on city congestion and air quality. Methods: In this paper, we conduct a comprehensive review of the performance of UDCs and investigate its alignment with the corporate governance (CG) and corporate social responsibility (CSR) initiatives, including the shareholders’ governance strategies and policies, as well as environmental, social, and economic measures. Our systematic literature review consists of multiple phases: In the first one, we utilize bibliometric tools to implement a quantitative analysis of the extant literature. Next, a cluster-based network analysis complements this analysis to describe the evolution of research in this area. Results: Our descriptive analysis categorizes existing research on UDCs based on CG- and CSR-compliant themes. We classify pertinent peer-reviewed articles into topical clusters and offer research opportunities related to improving the performance of UDCs. Conclusions: This study aims to stimulate further scholarly inquiry into sustainable city logistics and provides a knowledge-based guide for academicians and practitioners, logistics service providers, policymakers, and customers.
Electrospinning Mo-Doped Carbon Nanofibers as an Anode to Simultaneously Boost Bioelectrocatalysis and Extracellular Electron Transfer in Microbial Fuel Cells
Authors: Wu Xiaoshuai , Li Xiaofen , Shi Zhuanzhuan , Wang Xiaohai , Wang Zhikai , Li Chang Ming
Published: March 2023

The sluggish electron transfer at the interface of microorganisms and an electrode is a bottleneck of increasing the output power density of microbial fuel cells (MFCs). Mo-doped carbon nanofibers (Mo-CNFs) prepared with electrostatic spinning and high-temperature carbonization are used as an anode in MFCs here. Results clearly indicate that Mo2C nanoparticles uniformly anchored on carbon nanowire, and Mo-doped anodes could accelerate the electron transfer rate. The Mo-CNF ΙΙ anode delivered a maximal power density of 1287.38 mW m−2, which was twice that of the unmodified CNFs anode. This fantastic improvement mechanism is attributed to the fact that Mo doped on a unique nanofiber surface could enhance microbial colonization, electrocatalytic activity, and large reaction surface areas, which not only enable direct electron transfer, but also promote flavin-like mediated indirect electron transfer. This work provides new insights into the application of electrospinning technology in MFCs and the preparation of anode materials on a large scale.
Effect of Phosphogypsum Based Filler on the Performance of Asphalt Mortar and Mixture
Authors: Wan Jiuming , Han Tao , Li Kaifei , Shu Suxun , Hu Xiaodi , Gan Wenxia , Chen Zongwu
Published: March 2023

This study introduced phosphogypsum coupled with steel slag powder to prepare the phosphogypsum based filler (PF) for asphalt mixture. Penetration, penetration index, softening point, ductility, equivalent softening point, moisture stability of asphalt mortars with different steel slag powder content, filler-asphalt ratio, and PF content were studied. Mechanical properties of PF based asphalt mortar (P-AM) were then analyzed to determine the optimum steel slag content in PF. Overall desirability method was used to determine the optimum replacement ratio of PF content in limestone filler. Rheological properties of P-AM were also analyzed through dynamic shear rheometer. Volumetric performance, high-temperature performance, low-temperature performance, and moisture stability tests were carried out on PF based AC-20 asphalt mixture. Results showed that P-AM presented the optimum performance when the content of steel slag powder was 23% by mass of phosphogypsum. Fatigue and rutting factor of asphalt mortar were enhanced by PF. The optimum PF content in replacing limestone filler was 75% through overall desirability evaluation. PF developed the high-temperature performance and moisture stability of asphalt mixture. Additionally, volumetric and low-temperature performance were not significantly affected by PF. It is suggested that using PF which is based on phosphogypsum as a filler of asphalt mixture to partially replace traditional limestone filler was adequate.
Investigating the Impact of COVID-19 on E-Learning: Country Development and COVID-19 Response
Authors: Pejić Bach Mirjana , Jaković Božidar , Jajić Ivan , Meško Maja
Published: March 2023

Due to its severity, the outbreak of COVID-19 led to unprecedented levels of social isolation that affected educational institutions, among others. Digital technologies such as cloud computing and video broadcasting helped the adoption of e-learning during the crisis. However, the speed and efficiency of e-learning adoption during the COVID-19 period varied across countries. This paper compares the adoption of e-learning in European countries before and during the COVID-19 pandemic and the relationship between the pandemic, e-learning, and economic development. First, the adoption of e-learning in European countries before and during the pandemic is compared. Second, using fuzzy C-means clustering, homogeneous groups of European countries are formed based on e-learning indicators for the periods before and during the pandemic. Third, GDP per capita is used as an indicator of economic development and severity indices are used as an indicator of the severity of the response to the pandemic to compare the different clusters. The research results show that economically and digitally advanced countries led the adoption of e-learning in both the period before and the period during the pandemic. However, they also responded less strictly to the pandemic. Less-advanced countries responded more strictly to the pandemic, likely due to a lack of healthcare resources, and also fell behind in the adoption of e-learning.
Secret Key Distillation with Speech Input and Deep Neural Network-Controlled Privacy Amplification
Authors: Radomirović Jelica , Milosavljević Milan , Banjac Zoran , Jovanović Miloš
Published: March 2023

We propose a new high-speed secret key distillation system via public discussion based on the common randomness contained in the speech signal of the protocol participants. The proposed system consists of subsystems for quantization, advantage distillation, information reconciliation, an estimator for predicting conditional Renyi entropy, and universal hashing. The parameters of the system are optimized in order to achieve the maximum key distillation rate. By introducing a deep neural block for the prediction of conditional Renyi entropy, the lengths of the distilled secret keys are adaptively determined. The optimized system gives a key rate of over 11% and negligible information leakage to the eavesdropper, while NIST tests show the high cryptographic quality of produced secret keys. For a sampling rate of 16 kHz and quantization of input speech signals with 16 bits per sample, the system provides secret keys at a rate of 28 kb/s. This speed opens the possibility of wider application of this technology in the field of contemporary information security.
Maternal Periodontal Status as a Factor Influencing Obstetrical Outcomes
Authors: Völgyesi Petra , Radnai Márta , Németh Gábor , Boda Krisztina , Bernad Elena , Novák Tibor
Published: March 2023

Background and Objectives: Preterm birth as a complex phenomenon is influenced by numerous endogenic and exogenic factors, although its exact cause often remains obscure. According to epidemiological studies, maternal periodontal diseases, in addition to affecting general health, can also cause adverse pregnancy outcomes. Nonetheless, the existing results in the literature regarding this topic remain controversial. Consequently, our study aimed to determine the connection between poor maternal periodontal status and neonatal birth weight. Materials and Methods: A total of 111 primigravida–primiparous pregnant, healthy women underwent a periodontal examination in the second trimester of their pregnancies. Probing depth (PD) and bleeding on probing (BOP) were determined, and based on these diagnostic measurements, the patients were divided into three subgroups according to their dental status: healthy (H, n = 17), gingivitis (G, n = 67), and periodontitis (P, n = 27). Results: Considering that poor maternal oral status is an influencing factor for obstetrical outcomes, the presence of PD and BOP (characterized by the sulcus bleeding index, SBI) was evaluated. In the case of P, defined as PD ≥ 4 mm in at least one site and BOP ≥ 50% of the teeth, a significant correlation between BOP and a low neonatal birth weight at delivery (p = 0.001) was found. An analysis of the relationship between SBI and gestational age (GA) at the time of the periodontal examination in the different dental status groups showed a significant correlation between these parameters in the G group (p = 0.04). Conclusions: Our results suggest that a worse periodontal status during pregnancy may negatively affect obstetrical outcomes, especially the prematurity rate and newborn weight. Therefore, the importance of periodontal screening to prevent these complications is undeniable.
Health Information Technology Usability Evaluation Scale (Health-ITUES) and User-Experience Questionnaire (UEQ) for 3D Intraoperative Cognitive Navigation (ICON3DTM) System for Urological Procedures
Authors: Checcucci Enrico , Piramide Federico , De Cillis Sabrina , Volpi Gabriele , Piana Alberto , Verri Paolo , Bellin Andrea , Di Dio Michele , Fiori Cristian , Porpiglia Francesco , Amparore Daniele ,   Icon Study Group
Published: March 2023

Backgound and objectives: In recent years, the adoption of 3D models for surgical planning and intraoperative guidance has gained a wide diffusion. The aim of this study was to evaluate the surgeons’ perception and usability of ICON3DTM platform for robotic and laparoscopic urological surgical procedures. Materials and Methods: During the 10th edition of the Techno-Urology Meeting, surgeons and attendees had the opportunity to test the new ICON3DTM platform. The capability of the user to manipulate the model with hands/mouse, the software usability, the quality of the 3D model’s reproduction, and the quality of its use during the surgery were evaluated with the Health Information Technology Usability Evaluation Scale (Health-ITUES) and the User-Experience Questionnaire (UEQ). Results: Fifty-three participants responded to the questionnaires. Based on the answers to the Health-ITUES questionnaire, ICON3DTM resulted to have a positive additional value in presurgical/surgical planning with 43.4% and 39.6% of responders that rated 4 (agree) and 5 (strongly agree), respectively. Regarding the UEQ questionnaire, both mouse and infrared hand-tracking system resulted to be easy to use for 99% of the responders, while the software resulted to be easy to use for 93.4% of the responders. Conclusions: In conclusion, ICON3DTM has been widely appreciated by urologists thanks to its various applications, from preoperative planning to its support for intraoperative decision-making in both robot-assisted and laparoscopic settings.
Incidence and Predicting Factors of Histopathological Features at Robot-Assisted Radical Prostatectomy in the mpMRI Era: Results of a Single Tertiary Referral Center
Authors: Di Mauro Ernesto , Di Bello Francesco , Califano Gianluigi , Morra Simone , Creta Massimiliano , Celentano Giuseppe , Abate Marco , Fraia Agostino , Pezone Gabriele , Marino Claudio , Cilio Simone , Capece Marco , La Rocca Roberto , Imbimbo Ciro , Longo Nicola , Colla’ Ruvolo Claudia
Published: March 2023

Background and Objectives: To describe the predictors of cribriform variant status and perineural invasion (PNI) in robot-assisted radical prostatectomy (RARP) histology. To define the rates of upgrading between biopsy specimens and final histology and their possible predictive factors in prostate cancer (PCa) patients undergoing RARP. Material and Methods: Within our institutional database, 265 PCa patients who underwent prostate biopsies and consecutive RARP at our center were enrolled (2018–2022). In the overall population, two independent multivariable logistic regression models (LRMs) predicting the presence of PNI or cribriform variant status at RARP were performed. In low- and intermediate-risk PCa patients according to D’Amico risk classification, three independent multivariable LRMs were fitted to predict upgrading. Results: Of all, 30.9% were low-risk, 18.9% were intermediate-risk and 50.2% were high-risk PCa patients. In the overall population, the rates of the cribriform variant and PNI at RARP were 55.8% and 71.1%, respectively. After multivariable LRMs predicting PNI, total tumor length in biopsy cores (>24 mm [OR: 2.37, p-value = 0.03], relative to <24 mm) was an independent predictor. After multivariable LRMs predicting cribriform variant status, PIRADS (3 [OR:15.37], 4 [OR: 13.57] or 5 [OR: 16.51] relative to PIRADS 2, all p = 0.01) and total tumor length in biopsy cores (>24 mm [OR: 2.47, p = 0.01], relative to <24 mm) were independent predicting factors. In low- and intermediate-risk PCa patients, the rate of upgrading was 74.4% and 78.0%, respectively. After multivariable LRMs predicting upgrading, PIRADS (PIRADS 3 [OR: 7.01], 4 [OR: 16.98] or 5 [OR: 20.96] relative to PIRADS 2, all p = 0.01) was an independent predicting factor. Conclusions: RARP represents a tailored and risk-adapted treatment strategy for PCa patients. The indication of RP progressively migrates to high-risk PCa after a pre-operative assessment. Specifically, the PIRADS score at mpMRI should guide the decision-making process of urologists for PCa patients.
Morphological and Molecular Characterization of Eimeria spp. Infecting Domestic Poultry Gallus gallus in Riyadh City, Saudi Arabia
Authors: Mares Mohammed M. , Al-Quraishy Saleh , Abdel-Gaber Rewaida , Murshed Mutee
Published: March 2023

Coccidiosis in chickens is one of the major problems in the poultry industry, caused by protozoan parasites of the genus Eimeria. The current study used morphological and molecular characteristics to identify Eimeria spp. infecting domestic chickens (Gallus gallus) in the Riyadh region of Saudi Arabia. In this study, 120 domestic poultry were examined and 30 were found to be infected with oocysts of Eimeria spp. (25%). According to the morphology of the recorded oocysts, five species were found. Eimeria necatrix was the first species discovered, and it was distinguished by oblong, ovoid-shaped oocysts with double-layered walls that measured 20 (23–23) and 17 (16–20) μm. The second species was Eimeria maxima, which had oval- to egg-shaped oocysts with double-layered walls and measurements of 28 (26–29) and 23 (20–24) μm. The third species was Eimeria tenella, characterized by oval-shaped oocysts with double-layered walls and measurements of 21 (20–24) × 17 (16–20) μm. Eimeria praecox was the fourth species that was characterized by spherical-shaped oocysts with single-layered walls and measurements of 21 (19–23) × 20 (19–20) μm. Eimeria acervulina was the last species to have oval-shaped oocysts with double-layered walls and measurements of 20 (18–25) and 17 (14–20) μm. The percentages of infection with Eimeria species were as follows: E. tenella, 10.84%; E. necatrix, 5.84%; E. acervulina, 4.16%; E. maxima, 2.5%; and E. praecox, 1.66%. Nested PCR based on the amplification of internal transcribed spacer I (ITS-I) regions confirmed the presence of the five Eimeria species in the examined fecal samples with their specific amplicon sizes: E. necatrix (383 bp), E. maxima (145 bp), E. tenella (278 bp), E. praecopx (116 bp), and E. acervulina (321 bp).
Comparing Condensed and Hydrolysable Tannins for Mechanical Foaming of Furanic Foams: Synthesis and Characterization
Authors: Eckardt Jonas , Sepperer Thomas , Cesprini Emanuele , Šket Primož , Tondi Gianluca
Published: March 2023

This study examined the potential of hydrolysable tannin in comparison to condensed tannins for the production of furanic foams. The results indicate that chestnut tannin presents lower reactivity and requires a stronger acid for the polymerization. Additionally, foamability and density were found to be dependent on both surfactant concentration and tannin type, allowing lower densities for mimosa tannin and lower thermal conductivities for chestnut-based foams. Mimosa tannin was found to have the highest compression strength, followed by quebracho and chestnut, promising thermal conductivity of around 50 mW/m·K for 300 kg/m3 foams, which suggests that chestnut foams have the potential to performing highly when the density is reduced. Chemical analysis revealed that the methylene moieties of the furanics are non-specific and produces new covalent bonds with nucleophilic substrates: -OH groups and free-positions in the flavonoids. Overall, this study opens new perspectives for the application of hydrolysable tannins in polymer and material science.
Investigating the Interaction of an Anticancer Nucleolipidic Ru(III) Complex with Human Serum Proteins: A Spectroscopic Study
Authors: Riccardi Claudia , Campanella Antonella , Montesarchio Daniela , Del Vecchio Pompea , Oliva Rosario , Paduano Luigi
Published: March 2023

Ruthenium(III) complexes are very promising candidates as metal-based anticancer drugs, and several studies have supported the likely role of human serum proteins in the transport and selective delivery of Ru(III)-based compounds to tumor cells. Herein, the anticancer nanosystem composed of an amphiphilic nucleolipid incorporating a Ru(III) complex, which we named DoHuRu, embedded into the biocompatible cationic lipid DOTAP, was investigated as to its interaction with two human serum proteins thought to be involved in the mechanism of action of Ru(III)-based anticancer drugs, i.e., human serum albumin (HSA) and human transferrin (hTf). This nanosystem was studied in comparison with the simple Ru(III) complex named AziRu, a low molecular weight metal complex previously designed as an analogue of NAMI-A, decorated with the same ruthenium ligands as DoHuRu but devoid of the nucleolipid scaffold and not inserted in liposomal formulations. For this study, different spectroscopic techniques, i.e., Fluorescence Spectroscopy and Circular Dichroism (CD), were exploited, showing that DoHuRu/DOTAP liposomes can interact with both serum proteins without affecting their secondary structures.
Silylated-Acetylated Cyclodextrins as Chiral Sensors for the Enantiodiscrimination of Fluorinated Anesthetics
Authors: Recchimurzo Alessandra , Balzano Federica , Uccello Barretta Gloria , Gherardi Luca , Malanga Milo , Aiello Federica
Published: March 2023

Silylated-acetylated cyclodextrin (CD) derivatives have recently been investigated, via nuclear magnetic resonance (NMR) spectroscopy, as chiral sensors for substrates that are endowed and devoid of fluorine atoms, and the importance of Si-F interaction in the discrimination phenomena has been assessed. Here, the contributions of both superficial interactions and inclusion processes were further evaluated by extending the records to other chiral fluorinated substrates of interest for pharmaceutical applications. Non-equivalences were measured for both the 1H and 19F resonances in equimolar mixtures with the CDs; the promising results also supported the use of chiral sensors in sub-stoichiometric amounts. Finally, the occurrence of inclusion processes was evaluated by analyzing the intermolecular dipolar interactions by means of ROESY (Rotating-frame Overhauser Enhancement Spectroscopy) experiments. The study confirmed that the γCD derivative is the best chiral solvating agent for the fluorinated substrates investigated, likely due to the higher number of silyl moieties that can be involved in Si-F interactions. The contribution of inclusion processes to the enantiodiscrimination was also confirmed by comparison with the α- and β-analogues. Overall, the CD derivatives proved to be able to discriminate fluorinated substrates even when used in sub-stoichiometric amounts.
The Potential Mechanism of Cannabidiol (CBD) Treatment of Epilepsy in Pentetrazol (PTZ) Kindling Mice Uncovered by Multi-Omics Analysis
Authors: Lu Hongyuan , Wang Qinbiao , Jiang Xiaowen , Zhao Yanyun , He Miao , Wei Minjie
Published: March 2023

Cannabidiol (CBD) is the main active ingredient in the cannabis plant used for treating epilepsy and related diseases. However, how CBD ameliorates epilepsy and its effect on the hippocampus remains unknown. Herein, we evaluated how CBD ameliorates seizure degree in pentylenetetrazol (PTZ) induced epilepsy mice after being exposed to CBD (10 mg/kg p.o). In addition, transcriptome and metabolomic analysis were performed on the hippocampus. Our results suggested that CBD could alleviate PTZ-induced seizure, of which the NPTX2, Gprc5c, Lipg, and Stc2 genes were significantly down-regulated in mice after being exposed to PTZ. Transcriptome analysis showed 97 differently expressed genes (CBD) and the PTZ groups. Metabonomic analysis revealed that compared with the PTZ group, 41 up-regulated and 67 down-regulated metabolites were identified in the hippocampus of epileptic mice exposed to CBD. The correlation analysis for transcriptome and metabolome showed that (±) 15-HETE and carnitine C6:0 were at the core of the network and were involved in the positive or negative regulation of the related genes after being treated with CBD. In conclusion, CBD ameliorates epilepsy by acting on the metabolism, calcium signaling pathway, and tuberculosis pathways in the hippocampus. Our study provided a practical basis for the therapeutic potential of treating epilepsy using CBD.
The Unusual Architecture of RNA-Dependent RNA Polymerase (RdRp)’s Catalytic Chamber Provides a Potential Strategy for Combination Therapy against COVID-19
Authors: Metwally Kamel , Abo-Dya Nader E. , Alahmdi Mohammed Issa , Albalawi Maha Z. , Yahya Galal , Aljoundi Aimen , Salifu Elliasu Y. , Elamin Ghazi , Ibrahim Mahmoud A. A. , Sayed Yasien , Fanucchi Sylvia , Soliman Mahmoud E. S.
Published: March 2023

The unusual and interesting architecture of the catalytic chamber of the SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) was recently explored using Cryogenic Electron Microscopy (Cryo-EM), which revealed the presence of two distinctive binding cavities within the catalytic chamber. In this report, first, we mapped out and fully characterized the variations between the two binding sites, BS1 and BS2, for significant differences in their amino acid architecture, size, volume, and hydrophobicity. This was followed by investigating the preferential binding of eight antiviral agents to each of the two binding sites, BS1 and BS2, to understand the fundamental factors that govern the preferential binding of each drug to each binding site. Results showed that, in general, hydrophobic drugs, such as remdesivir and sofosbuvir, bind better to both binding sites than relatively less hydrophobic drugs, such as alovudine, molnupiravir, zidovudine, favilavir, and ribavirin. However, suramin, which is a highly hydrophobic drug, unexpectedly showed overall weaker binding affinities in both binding sites when compared to other drugs. This unexpected observation may be attributed to its high binding solvation energy, which disfavors overall binding of suramin in both binding sites. On the other hand, hydrophobic drugs displayed higher binding affinities towards BS1 due to its higher hydrophobic architecture when compared to BS2, while less hydrophobic drugs did not show a significant difference in binding affinities in both binding sites. Analysis of binding energy contributions revealed that the most favorable components are the ΔEele, ΔEvdw, and ΔGgas, whereas ΔGsol was unfavorable. The ΔEele and ΔGgas for hydrophobic drugs were enough to balance the unfavorable ΔGsol, leaving the ΔEvdw to be the most determining factor of the total binding energy. The information presented in this report will provide guidelines for tailoring SARS-CoV-2 inhibitors with enhanced binding profiles.
Monitoring the Evolution of the Aroma Profile of Lager Beer in Aluminium Cans and Glass Bottles during the Natural Ageing Process by Means of HS-SPME/GC-MS and Multivariate Analysis
Authors: de Lima Ana Carolina , Aceña Laura , Mestres Montserrat , Boqué Ricard
Published: March 2023

Headspace solid-phase microextraction coupled to gas chromatography-mass spectrometry (HS-SPME/GC-MS), sensory evaluation, and multivariate analysis were applied to monitor and compare the evolution of the aromatic profile of a lager beer in different types of containers (aluminum cans and glass bottles) during the natural ageing process. Samples were aged naturally for a year in the absence of light with a controlled temperature of around 14 °C +/− 0.5 °C. The sensory evaluation applied was a blind olfactometric triangle test between canned and bottled samples at different periods of aging: fresh, 6 months, and 11 months. The sensory evaluation showed that the panelists were able to differentiate between samples, except for the fresh samples from the brewery. A total of 34 volatile compounds were identified using the HS-SPME/GC-MS technique for both packaging types in this experiment. The application of multivariate analysis to the GC-MS data showed that the samples could not be differentiated according to the type of packaging but could be differentiated by the ageing time. The results showed that the combination of sensory, HS-SPME-GC-MS, and multivariate analysis seemed to be a valuable tool for monitoring and identifying possible changes in the aroma profile of a beer during its shelf life. Furthermore, the results showed that storing beer under optimal conditions helped preserve its quality during its shelf life, regardless of the type of packaging (aluminum can and glass bottle).
One-Step Microwell Plate-Based Spectrofluorimetric Assay for Direct Determination of Statins in Bulk Forms and Pharmaceutical Formulations: A Green Eco-Friendly and High-Throughput Analytical Approach
Authors: Darwish Ibrahim A. , Darwish Hany W. , Alzoman Nourah Z. , Ali Awadh M.
Published: March 2023

This study describes the development of a one-step microwell spectrofluorimetric assay (MW-SFA) with high sensitivity and throughput for the determination of four statins in their pharmaceutical and formulations (tablets). These statins were pitavastatin (PIT), fluvastatin (FLU), rosuvastatin (ROS) and atorvastatin (ATO). The MW-SFA involves the measurement of the native fluorescence of the statin aqueous solutions. The assay was conducted in white opaque 96-microwell plates, and the fluorescence intensities of the solutions were measured by using a fluorescence microplate reader. The optimum conditions of the assay were established; under which, linear relationships with good correlation coefficients (0.9991–0.9996) were found between the fluorescence intensity and the concentration of the statin drug in a range of 0.2–200 µg mL–1 with limits of detection in a range of 0.1–4.1 µg mL–1. The proposed MW-SFA showed high precision, as the values of the relative standard deviations did not exceed 2.5%. The accuracy of the assay was proven by recovery studies, as the recovery values were 99.5–101.4% (±1.4–2.1%). The assay was applied to the determination of the investigated statins in their tablets. The results were statistically compared with those obtained by a reference method and the results proved to have comparable accuracy and precision of both methods, as evidenced by the t- and F-tests, respectively. The green and eco-friendly feature of the proposed assay was assessed by four different metric tools, and all the results proved that the assay meets the requirements of green and eco-friendly analytical approaches. In addition, ever-increasing miniaturization as handling of large numbers of micro-volume samples simultaneously in the proposed assay gave it a high-throughput feature. Therefore, the assay is a valuable tool for the rapid routine application in the pharmaceutical quality control units for the determination of statins.
Novel Amperometric Mercury-Selective Sensor Based on Organic Chelator Ionophore
Authors: Elsebai Basant , Ghica Mariana Emilia , Abbas Mohammed Nooredeen , Brett Christopher M. A.
Published: March 2023

A novel amperometric sensor for the direct determination of toxic mercury ions, Hg2+, based on the organic chelator ionophore N, N di (2-hydroxy-5-[(4-nitrophenyl)diazenyl]benzaldehyde) benzene-1,2-diamine (NDBD), and multiwalled carbon nanotubes (MWCNT) immobilized on a glassy carbon electrode surface was developed. The parameters influencing sensor performance including the ionophore concentration, the applied potential, and electrolyte pH were optimized. The sensor response to Hg2+ was linear between 1–25 µM with a limit of detection of 60 nM. Interferences from other heavy metal ions were evaluated and the sensor showed excellent selectivity towards Hg2+. The method was successfully applied to the determination of mercury ions in milk and water samples.
Performance Enhancement of Proton Exchange Membrane Fuel Cell through Carbon Nanofibers Grown In Situ on Carbon Paper
Authors: Liu Chang , Li Shang
Published: March 2023

We developed an integrated gas diffusion layer (GDL) for proton exchange membrane (PEM) fuel cells by growing carbon nanofibers (CNFs) in situ on carbon paper via the electro-polymerization of polyaniline (PANI) on carbon paper followed by a subsequent carbonization treatment process. The CNF/carbon paper showed a microporous structure and a significantly increased pore volume compared to commercial carbon paper. By utilizing this CNF/carbon paper in a PEM fuel cell, it was found that the cell with CNF/carbon paper had superior performance compared to the commercial GDL at both high and low humidity conditions, and its power density was as high as 1.21 W cm−2 at 100% relative humidity, which is 26% higher than that of a conventional gas diffusion layer (0.9 W cm−2). The significant performance enhancement was attributed to a higher pore volume and porosity of the CNF/carbon paper, which improved gas diffusion in the GDL. In addition, the superior performance of the cell with CNF/carbon paper at low relative humidity demonstrated that it had better water retention than the commercial GDL. This study provides a novel and facile method for the surface modification of GDLs to improve the performance of PEM fuel cells. The CNF/carbon paper with a microporous structure has suitable hydrophobicity and lower through-plane resistance, which makes it promising as an advanced substrate for GDLs in fuel cell applications.
Understanding the Potential Role of Nanotechnology in Liver Fibrosis: A Paradigm in Therapeutics
Authors: Singh Sukhbir , Sharma Neelam , Shukla Saurabh , Behl Tapan , Gupta Sumeet , Anwer Md. Khalid , Vargas-De-La-Cruz Celia , Bungau Simona Gabriela , Brisc Cristina
Published: March 2023

The liver is a vital organ that plays a crucial role in the physiological operation of the human body. The liver controls the body’s detoxification processes as well as the storage and breakdown of red blood cells, plasma protein and hormone production, and red blood cell destruction; therefore, it is vulnerable to their harmful effects, making it more prone to illness. The most frequent complications of chronic liver conditions include cirrhosis, fatty liver, liver fibrosis, hepatitis, and illnesses brought on by alcohol and drugs. Hepatic fibrosis involves the activation of hepatic stellate cells to cause persistent liver damage through the accumulation of cytosolic matrix proteins. The purpose of this review is to educate a concise discussion of the epidemiology of chronic liver disease, the pathogenesis and pathophysiology of liver fibrosis, the symptoms of liver fibrosis progression and regression, the clinical evaluation of liver fibrosis and the research into nanotechnology-based synthetic and herbal treatments for the liver fibrosis is summarized in this article. The herbal remedies summarized in this review article include epigallocathechin-3-gallate, silymarin, oxymatrine, curcumin, tetrandrine, glycyrrhetinic acid, salvianolic acid, plumbagin, Scutellaria baicalnsis Georgi, astragalosides, hawthorn extract, and andrographolides.
Cytotoxicity against A549 Human Lung Cancer Cell Line via the Mitochondrial Membrane Potential and Nuclear Condensation Effects of Nepeta paulsenii Briq., a Perennial Herb
Authors: Hanif Aqsa , Ibrahim Ahmad H. , Ismail Sidra , Al-Rawi Sawsan S. , Ahmad Jam Nazeer , Hameed Mansoor , Mustufa Ghulam , Tanwir Samina
Published: March 2023

The genus Nepeta belongs to the largest Lamiaceae family, with 300 species, which are distributed throughout the various regions of Africa, Asia, India, and America. Along with other plant families distinguished by their medicinal and therapeutic values, the Nepeta genus of Lameaceae remains relatively valuable. Hence, the phytochemicals of N. paulsenii Briq. were extracted using different plant parts, i.e., leaves, stem, roots, flowers, and the whole plant by using various solvents (ethanol, water, and ethyl acetate), obtaining 15 fractions. Each extract of dried plant material was analyzed by FT-IR and GC-MS to identify the chemical constituents. The cytotoxicity of each fraction was analyzed by MTT assay and mitochondrial membrane potential and nuclear condensation assays against lung cancer cells. Among the ethyl acetate and ethanolic extracts, the flowers showed the best results, with IC50 values of 51.57 μg/mL and 50.58 μg/mL, respectively. In contrast, among the water extracts of the various plant segments, the stem showed the best results, with an IC50 value of 123.80 μg/mL. 5-flourouracil was used as the standard drug, providing an IC50 value of 83.62 μg/mL. The Hoechst 33342 stain results indicated apoptotic features, i.e., chromatin dissolution and broken down, fragmented, and crescent-shaped nuclei. The ethanolic extracts of the flowers showed more pronounced apoptotic effects on the cells. The mitochondrial membrane potential indicated that rhodamine 123 fluorescence signals suppressed mitochondrial potential due to the treatment with the extracts. Again, the apoptotic index of the ethanolic extract of the flowers remained the highest. Hence it can be concluded that the flower part of N. paulsenii Briq. was found to be the most active against the A459 human lung cancer cell line.
Assessment of Lipophilicity Parameters of Antimicrobial and Immunosuppressive Compounds
Authors: Wardecki Dawid , Dołowy Małgorzata , Bober-Majnusz Katarzyna
Published: March 2023

Lipophilicity in addition to the solubility, acid-base character and stability is one of the most important physicochemical parameters of a compound required to assess the ADMET properties (absorption, distribution, metabolism, excretion and toxicity) of a bioactive molecule. Therefore, the subject of this work was to determine the lipophilicity parameters of selected antimicrobial and immunosuppressive compounds such as delafloxacin, linezolid, sutezolid, ceftazidime, everolimus and zotarolimus using thin-layer chromatography in reversed phase system (RP-TLC). The chromatographic parameters of lipophilicity (RMW) for tested compounds were determined on different stationary phases: RP18F254, RP18WF254 and RP2F254 using ethanol, acetonitrile, and propan-2-ol as organic modifiers of mobile phases used. Chromatographically established RMW values were compared with partition coefficients obtained by different computational methods (AlogPs, AClogP, AlogP, MlogP, XlogP2, XlogP3, logPKOWWIN, ACD/logP, milogP). Both cluster and principal component analysis (CA and PCA) of the received results allowed us to compare the lipophilic nature of the studied compounds. The sum of ranking differences analysis (SRD) of all lipophilicity parameters was helpful to select the most effective method of determining the lipophilicity of the investigated compounds. The presented results demonstrate that RP-TLC method may be a good tool in determining the lipophilic properties of studied substances. Obtained lipophilic parameters of the compounds can be valuable in the design of their new derivatives as efficient antimicrobial and immunosuppressive agents.
Biosurfactants: Forthcomings and Regulatory Affairs in Food-Based Industries
Authors: Sharma Deepansh , Singh Deepti , Sukhbir-Singh Gadhwal Monika , Karamchandani Bhoomika M. , Aseri Gajender Kumar , Banat Ibrahim M. , Satpute Surekha K.
Published: March 2023

The terms discussed in this review—biosurfactants (BSs) and bioemulsifiers (BEs)—describe surface-active molecules of microbial origin which are popular chemical entities for many industries, including food. BSs are generally low-molecular-weight compounds with the ability to reduce surface tension noticeably, whereas BEs are high-molecular-weight molecules with efficient emulsifying abilities. Some other biomolecules, such as lecithin and egg yolk, are useful as natural BEs in food products. The high toxicity and severe ecological impact of many chemical-based surfactants have directed interest towards BSs/BEs. Interest in food surfactant formulations and consumer anticipation of “green label” additives over synthetic or chemical-based surfactants have been steadily increasing. BSs have an undeniable prospective for replacing chemical surfactants with vast significance to food formulations. However, the commercialization of BSs/BEs production has often been limited by several challenges, such as the optimization of fermentation parameters, high downstream costs, and low yields, which had an immense impact on their broader adoptions in different industries, including food. The foremost restriction regarding the access of BSs/BEs is not their lack of cost-effective industrial production methods, but a reluctance regarding their potential safety, as well as the probable microbial hazards that may be associated with them. Most research on BSs/BEs in food production has been restricted to demonstrations and lacks a comprehensive assessment of safety and risk analysis, which has limited their adoption for varied food-related applications. Furthermore, regulatory agencies require extensive exploration and analysis to secure endorsements for the inclusion of BSs/BEs as potential food additives. This review emphasizes the promising properties of BSs/BEs, trailed by an overview of their current use in food formulations, as well as risk and toxicity assessment. Finally, we assess their potential challenges and upcoming future in substituting chemical-based surfactants.
High-Branched Organosilicon Epoxy Resin with Low Viscosity, Excellent Toughness, Hydrophobicity, and Dielectric Property
Authors: Yu Min , Chen Zeyuan , Li Jie , Tan Jihuai , Zhu Xinbao
Published: March 2023

Rapidly developing technology places higher demands on materials, thus the simultaneous improvement of materials’ multiple properties is a hot research topic. In this work, a high-branched silicone epoxy resin (QSiE) was synthesized and applied to the curing system of bisphenol A epoxy resin (DGEBA) for modification investigations. When 6 wt% QSiE was added to the system, the viscosity dropped by 51.8%. The mechanical property testing results indicated that QSiE could significantly enhance the material’s toughness while preserving good rigidity. The impact strength was enhanced by 1.31 times when 6wt% of QSiE was introduced. Additionally, the silicon skeleton in QSiE has low surface energy and low polarizability, which could endow the material with good hydrophobic and dielectric properties. This work provided a new idea for the preparation of high-performance epoxy resin additives, and provided a broad prospect for cutting-edge applications of epoxy resins.