bluuebunny/crossref_metadata_2025 huggingface dataset:
The Combination of Gold and Silver Food Nanoparticles with Gluten Peptides Alters the Autophagic Pathway in Intestinal Crypt-like Cells
Authors: Mancuso Clara , Tremblay Eric , Gnodi Elisa , Jean Steve , Beaulieu Jean-François , Barisani Donatella
Published: August 2023

Metallic nanoparticles (mNPs) are widely used as food additives and can interact with gliadin triggering an immune response, but evaluation of the effects on crypts, hypertrophic in celiac subjects, is still lacking. This study evaluated the effects of gold and silver mNPs in combination with gliadin on crypt-like cells (HIEC-6). Transmission electron microscopy (TEM) was used to evaluate gliadin-mNP aggregates in cells. Western blot and immunofluorescence analysis assessed autophagy-related molecule levels (p62, LC3, beclin-1, EGFR). Lysosome functionality was tested with acridine orange (AO) and Magic Red assays. TEM identified an increase in autophagic vacuoles after exposure to gliadin + mNPs, as also detected by significant increments in LC3-II and p62 expression. Immunofluorescence confirmed the presence of mature autophagosomes, showing LC3 and p62 colocalization, indicating an altered autophagic flux, further assessed with EGFR degradation, AO and Magic Red assays. The results showed a significant reduction in lysosomal enzyme activity and a modest reduction in acidity. Thus, gliadin + mNPs can block the autophagic flux inducing a lysosomal defect. The alteration of this pathway, essential for cell function, can lead to cell damage and death. The potential effects of this copresence in food should be further characterized to avoid a negative impact on celiac disease subjects.
Complete Chloroplast Genomes and Phylogenetic Relationships of Bougainvillea spectabilis and Bougainvillea glabra (Nyctaginaceae)
Authors: Zhang Huihui , Huang Tao , Zhou Qi , Sheng Qianqian , Zhu Zunling
Published: August 2023

Bougainvillea L. (Nyctaginaceae) is a South American native woody flowering shrub of high ornamental, economic, and medicinal value which is susceptible to cold damage. We sequenced the complete chloroplast (cp) genome of B. glabra and B. spectabilis, two morphologically similar Bougainvillea species differing in cold resistance. Both genomes showed a typical quadripartite structure consisting of one large single-copy region, one small single-copy region, and two inverted repeat regions. The cp genome size of B. glabra and B. spectabilis was 154,520 and 154,542 bp, respectively, with 131 genes, including 86 protein-coding, 37 transfer RNA, and 8 ribosomal RNA genes. In addition, the genomes contained 270 and 271 simple sequence repeats, respectively, with mononucleotide repeats being the most abundant. Eight highly variable sites (psbN, psbJ, rpoA, rpl22, psaI, trnG-UCC, ndhF, and ycf1) with high nucleotide diversity were identified as potential molecular markers. Phylogenetic analysis revealed a close relationship between B. glabra and B. spectabilis. These findings not only contribute to understanding the mechanism by which the cp genome responds to low-temperature stress in Bougainvillea and elucidating the evolutionary characteristics and phylogenetic relationships among Bougainvillea species, but also provide important evidence for the accurate identification and breeding of superior cold-tolerant Bougainvillea cultivars.
Genetic Variation and Sensory Perception of a Pediatric Formulation of Ibuprofen: Can a Medicine Taste Too Good for Some?
Authors: Mennella Julie A. , Kan Mengyuan , Lowenthal Elizabeth D. , Saraiva Luis R. , Mainland Joel D. , Himes Blanca E. , Pepino M. Yanina
Published: August 2023

There is wide variation in how individuals perceive the chemosensory attributes of liquid formulations of ibuprofen, encompassing both adults and children. To understand personal variation in the taste and chemesthesis properties of this medicine, and how to measure it, our first scientific strategy centered on utilizing trained adult panelists, due to the complex and time-consuming psychophysical tasks needed at this initial stage. We conducted a double-blind cohort study in which panelists underwent whole-genome-wide genotyping and psychophysically evaluated an over-the-counter pediatric medicine containing ibuprofen. Associations between sensory phenotypes and genetic variation near/within irritant and taste receptor genes were determined. Panelists who experienced the urge to cough or throat sensations found the medicine less palatable and sweet, and more irritating. Perceptions varied with genetic ancestry; panelists of African genetic ancestry had fewer chemesthetic sensations, rating the medicine sweeter, less irritating, and more palatable than did those of European genetic ancestry. We discovered a novel association between TRPA1 rs11988795 and tingling sensations, independent of ancestry. We also determined for the first time that just tasting the medicine allowed predictions of perceptions after swallowing, simplifying future psychophysical studies on diverse populations of different age groups needed to understand genetic, cultural–dietary, and epigenetic factors that influence individual perceptions of palatability and, in turn, adherence and the risk of accidental ingestion.
Reactive Oxygen Species: A Crosslink between Plant and Human Eukaryotic Cell Systems
Authors: Guo Wei , Xing Yadi , Luo Xiumei , Li Fuguang , Ren Maozhi , Liang Yiming
Published: August 2023

Reactive oxygen species (ROS) are important regulating factors that play a dual role in plant and human cells. As the first messenger response in organisms, ROS coordinate signals in growth, development, and metabolic activity pathways. They also can act as an alarm mechanism, triggering cellular responses to harmful stimuli. However, excess ROS cause oxidative stress-related damage and oxidize organic substances, leading to cellular malfunctions. This review summarizes the current research status and mechanisms of ROS in plant and human eukaryotic cells, highlighting the differences and similarities between the two and elucidating their interactions with other reactive substances and ROS. Based on the similar regulatory and metabolic ROS pathways in the two kingdoms, this review proposes future developments that can provide opportunities to develop novel strategies for treating human diseases or creating greater agricultural value.
BRD4 Inhibition Enhances the Antitumor Effects of Radiation Therapy in a Murine Breast Cancer Model
Authors: Kim Seongmin , Jeon Seung Hyuck , Han Min Guk , Kang Mi Hyun , Kim In Ah
Published: August 2023

Bromodomain-containing protein 4 (BRD4) is an intracellular protein that regulates expression of various cellular functions. This study investigated whether BRD4 inhibition can alter the immunomodulatory and antitumor effects of radiation therapy (RT). A murine breast cancer cell line was implanted into BALB/c mice. The dual-tumor model was used to evaluate the abscopal effects of RT. A total of 24 Gy was delivered and BRD4 inhibitor was injected intravenously. Tumor size was measured, and in vivo imaging was performed to evaluate tumor growth. Flow cytometry and immunohistochemistry were performed to examine immunologic changes upon treatment. The combination of BRD4 inhibitor and RT significantly suppressed tumor growth compared to RT alone. BRD4 inhibitor reduced the size of the unirradiated tumor, indicating that it may induce systemic immune responses. The expression of HIF-1α and PD-L1 in the tumor was significantly downregulated by the BRD4 inhibitor. The proportion of M1 tumor-associated macrophages (TAMs) increased, and the proportion of M2 TAMs decreased upon BRD4 inhibition. BRD4 inhibitor expanded CD4+ and CD8+ T cell populations in the tumor microenvironment. Additionally, splenic monocytic myeloid derived suppressor cells, which were increased by RT, were reduced upon the addition of BRD4 inhibitor. Therefore, the addition of BRD4 inhibitor significantly enhanced the systemic antitumor responses of local RT.
Graph-Based Extractive Text Summarization Sentence Scoring Scheme for Big Data Applications
Authors: Verma Jai Prakash , Bhargav Shir , Bhavsar Madhuri , Bhattacharya Pronaya , Bostani Ali , Chowdhury Subrata , Webber Julian , Mehbodniya Abolfazl
Published: August 2023

The recent advancements in big data and natural language processing (NLP) have necessitated proficient text mining (TM) schemes that can interpret and analyze voluminous textual data. Text summarization (TS) acts as an essential pillar within recommendation engines. Despite the prevalent use of abstractive techniques in TS, an anticipated shift towards a graph-based extractive TS (ETS) scheme is becoming apparent. The models, although simpler and less resource-intensive, are key in assessing reviews and feedback on products or services. Nonetheless, current methodologies have not fully resolved concerns surrounding complexity, adaptability, and computational demands. Thus, we propose our scheme, GETS, utilizing a graph-based model to forge connections among words and sentences through statistical procedures. The structure encompasses a post-processing stage that includes graph-based sentence clustering. Employing the Apache Spark framework, the scheme is designed for parallel execution, making it adaptable to real-world applications. For evaluation, we selected 500 documents from the WikiHow and Opinosis datasets, categorized them into five classes, and applied the recall-oriented understudying gisting evaluation (ROUGE) parameters for comparison with measures ROUGE-1, 2, and L. The results include recall scores of 0.3942, 0.0952, and 0.3436 for ROUGE-1, 2, and L, respectively (when using the clustered approach). Through a juxtaposition with existing models such as BERTEXT (with 3-gram, 4-gram) and MATCHSUM, our scheme has demonstrated notable improvements, substantiating its applicability and effectiveness in real-world scenarios.
Early versus Delayed Surgery in Patients with Left-Sided Infective Endocarditis and Stroke
Authors: Kremer Jamila , Jahn Joshua , Klein Sabrina , Farag Mina , Borst Tobias , Karck Matthias
Published: August 2023

Background: Timing of surgery remains controversial in patients with infective endocarditis and stroke. Guidelines on infective endocarditis suggest delaying surgery for up to 4 weeks. However, with early heart failure due to progression of the infection or recurrent septic embolism, urgent surgery becomes imperative. Methods: Out of 688 patients who were surgically treated for left-sided infective endocarditis, 187 presented with preoperative neurological events. The date of cerebral stroke onset was documented in 147 patients. The patients were stratified according to timing of surgery: 61 in the early group (0–7 days) vs. 86 in the delayed group (>7 days). Postoperative neurological outcome was assessed by the modified Rankin Scale. Results: Preoperative sepsis was more prevalent in patients with preoperative neurological complications (46.0% vs. 29.5%, p < 0.001). Patients with haemorrhagic stroke were operated on later (19.8% vs. 3.3%, p = 0.003). Postoperative cerebrovascular accidents were comparable between both groups (p = 0.13). Overall, we observed good neurological outcomes (p = 0.80) and a high recovery rate, with only 5% of cases showing neurological deterioration after surgery (p = 0.29). In-hospital mortality and long-term survival were not significantly different in the early and delayed surgery groups (log-rank, p = 0.22). Conclusions: Early valve surgery in high-risk patients with infective endocarditis and stroke can be performed safely and is not associated with worse outcomes.
The Associations between Cognitive Function, Depressive Symptoms, and Contact with Adult Children in Older Couples
Authors: Min Jin-young , Kim Beom , Min Kyoung-bok
Published: August 2023

The aim of this study was to investigate the association between cognitive function and depressive symptoms in older couples while also examining the relationship between cognitive performance and the frequency of contact with adult children. A total of 96 couples volunteered for this study and provided their informed consent at enrollment. Participants completed a neuropsychological test battery consisting of five cognitive domains: attention, language and related functions, visuospatial functions, memory, and frontal/executive functions. Symptoms of depression were assessed using the short version of the Geriatric Depression Scale. The number of contacts with children was categorized into ≥1 per month and <1 per month. We found that the cognitive functions of husbands with depressed wives were significantly lower in the frontal/executive functions. In contrast, the wives’ cognitive performance was not associated with the husbands’ depressive symptoms. For couples who had contact with their adult children less than once a month, the odds of the husbands with lower cognitive performance were significantly higher, which was reflected in their scores in visuospatial and executive functions. Among older married couples, the cognitive functions of husbands may be influenced more by their wives’ mental health and degree of contact with their adult children. This infers that wives and offspring may act as a buffer against the cognitive impairment of older married men.
Intrauterine Tamponade Balloon for Management of Severe Postpartum Haemorrhage: Does Early Insertion Change the Outcome? A Retrospective Study on Blood Loss
Authors: Futcher Françoise , Moufawad Graziella , Centini Gabriele , Hayek Jad , Tarchichi Jana , Bakar Joseph , Habib Nassir
Published: August 2023

The French College of Gynecologists and Obstetricians (CNGOF) recommends the use of intrauterine tamponade balloon (IUTB) in postpartum haemorrhage for bleeding that is refractory after sulprostone before either surgery or interventional radiology. However, the elapsed time between uterotonic drug injection and the insertion of intrauterine tamponade balloon was not reliably assessed. Objective: To evaluate the role of the timing of IUTB insertion and to assess the correlation between the time of insertion and outcome. Methods: A retrospective study in two tertiary care centres, including patients transferred for severe PPH management. Results: A total of 81 patients were included: 52 patients with IUTB inserted before 15 min (group 1) and 29 patients with IUTB inserted after 15 min (group 2). The mean volume of blood loss in the group of patients with IUTB inserted before 15 min was significantly lower than in group of patients with IUTB set after 15 min. Conclusion: An IUTB could be inserted simultaneously with a uterotonic agent, within 15 min and not after 15 min as suggested by local guidelines, but further prospective studies are required to confirm this.
Hemi-Ultrathin Descemet Stripping Automated Endothelial Keratoplasty (Hemi-UT-DSAEK) Using Pediatric Donor Corneas: A Case Series
Authors: Leon Pia , Francescutti Lorena , Gentile Pietro , Birattari Federica , Ponzin Diego , Camposampiero Davide , Franch Antonella , Parekh Mohit
Published: August 2023

Objective: We sought to evaluate the clinical outcomes of hemi-UT-DSAEK grafts from the pediatric donor corneas of patients affected by Fuchs Endothelial Corneal Dystrophy (FECD). Methods: A prospective, interventional case series was conducted at the Ophthalmology Department of Venice Civil Hospital and the Veneto Eye Bank Foundation (Venice, Italy). Six eyes of six patients affected by FECD received large-diameter, semicircular hemi-UT-DSAEK grafts obtained from three pediatric donor corneas using the standard pull-through method. Endothelial cell density (ECD), central corneal thickness (CCT), best-corrected visual acuity (BCVA) and intraoperative and postoperative complications were recorded at different time intervals up to 12 months. Results: The average donor age was 64.6 ± 8.6 years, and the pre-operative ECD was 3266 ± 225 cells/mm2. At 12 months postoperatively, the average ECD was 1376 ± 509 cells/mm2 with a mean decrease of 56.8 ± 19.1% from the preoperative donor count. At 12 months, four out of six eyes had significantly improved and reached a BCVA of ≥20/25 (Snellen equivalent). The mean CCT significantly decreased from 788 ± 138 μm before surgery to 576 ± 30 μm at 12 months postoperatively (p < 0.01). Conclusions: Hemi-UT-DSAEK grafts using pediatric donor corneas are surgically feasible and can provide similar clinical outcomes compared to conventional UT-DSAEK. Transplanting pediatric donor tissues with high ECD into two patients could potentially increase the donor tissue pool to treat endothelial disease.
Association between Non-Steroidal Anti-Inflammatory Drug Use and Major Cardiovascular Outcomes in Patients with Acute Coronary Syndrome in the Arabian Gulf
Authors: Al-Zakwani Ibrahim , Al-Maqbali Juhaina Salim , AlMahmeed Wael , AlRawahi Najib , Al-Asmi Abdullah , Zubaid Mohammad
Published: August 2023

Objectives: Studies on the association between non-steroidal anti-inflammatory drugs (NSAIDs) and major adverse cardiovascular events (MACE) in the Arabian Gulf are scarce. The aim of this study was to evaluate the association between NSAIDs use and MACE in acute coronary syndrome (ACS) patients in the Arabian Gulf region. Methods: Data were analyzed from 3007 consecutive patients diagnosed with ACS admitted to 29 hospitals in four Arabian Gulf countries from January 2012 to January 2013, as well as being on prior NSAIDs use during the index admission. The MACE included stroke/transient ischemic attacks (TIAs), myocardial infarction (MI), all-cause mortality and readmissions for cardiac reasons. Results: The overall mean age of the cohort was 62 ± 12 years, and 9.6% (n = 290) of the patients were on prior NSAID use during the index admission. At 12-months follow-up, after adjusting for confounding factors, patients on NSAIDs were significantly more likely to have had MACE (adjusted OR (aOR), 1.89; 95% confidence interval (CI): 1.44–2.48; p < 0.001). Specifically, the higher event rates observed were stroke/TIA (aOR, 2.50; 95% CI: 1.51–4.14; p < 0.001) and readmissions for cardiac reasons (aOR, 2.09; 95% CI: 1.59–2.74; p < 0.001), but not MI (aOR, 1.26; 95% CI: 0.80–1.99; p = 0.320) and all-cause mortality (aOR, 0.79; 95% CI: 0.46–1.34; p = 0.383). Conclusions: NSAIDs use was associated with significant stroke/TIA events as well as readmissions for cardiac reasons. However, NSAIDs were not associated with increased MIs or all-cause mortality rates in patients with ACS in the Arabian Gulf.
Testicular Evaluation Using Shear Wave Elastography (SWE) in Patients with Varicocele
Authors: Baleato-Gonzalez Sandra , Osorio-Vazquez Iria , Flores-Ríos Enrique , Santiago-Pérez María Isolina , Laguna-Reyes Juan Pablo , Garcia-Figueiras Roberto
Published: August 2023

Purpose: To assess the possible influence of the presence of varicocele on the quantification of testicular stiffness. Methods: Ultrasound with shear wave elastography (SWE) was performed on 48 consecutive patients (96 testicles) referred following urology consultation for different reasons. A total of 94 testes were studied and distributed in three groups: testes with varicocele (group A, n = 19), contralateral normal testes (group B; n = 13) and control group (group C, n = 62). Age, testicular volume and testicular parenchymal tissue stiffness values of the three groups were compared using the Kruskal–Wallis test. Results: The mean age of the patients was 42.1 ± 11.1 years. The main reason for consultation was infertility (64.6%). The mean SWE value was 4 ± 0.4 kPa (kilopascal) in group A, 4 ± 0.5 kPa in group B and 4.2 ± 0.7 kPa in group C or control. The testicular volume was 15.8 ± 3.8 mL in group A, 16 ± 4.3 mL in group B and 16.4 ± 5.9 mL in group C. No statistically significant differences were found between the three groups in terms of age, testicular volume and tissue stiffness values. Conclusion: Tissue stiffness values were higher in our control group (healthy testicles) than in patients with varicocele.
Clinical Validation Benchmark Dataset and Expert Performance Baseline for Colorectal Polyp Localization Methods
Authors: Sánchez-Peralta Luisa F. , Glover Ben , Saratxaga Cristina L. , Ortega-Morán Juan Francisco , Nazarian Scarlet , Picón Artzai , Pagador J. Blas , Sánchez-Margallo Francisco M.
Published: August 2023

Colorectal cancer is one of the leading death causes worldwide, but, fortunately, early detection highly increases survival rates, with the adenoma detection rate being one surrogate marker for colonoscopy quality. Artificial intelligence and deep learning methods have been applied with great success to improve polyp detection and localization and, therefore, the adenoma detection rate. In this regard, a comparison with clinical experts is required to prove the added value of the systems. Nevertheless, there is no standardized comparison in a laboratory setting before their clinical validation. The ClinExpPICCOLO comprises 65 unedited endoscopic images that represent the clinical setting. They include white light imaging and narrow band imaging, with one third of the images containing a lesion but, differently to another public datasets, the lesion does not appear well-centered in the image. Together with the dataset, an expert clinical performance baseline has been established with the performance of 146 gastroenterologists, who were required to locate the lesions in the selected images. Results shows statistically significant differences between experience groups. Expert gastroenterologists’ accuracy was 77.74, while sensitivity and specificity were 86.47 and 74.33, respectively. These values can be established as minimum values for a DL method before performing a clinical trial in the hospital setting.
AI for Psychometrics: Validating Machine Learning Models in Measuring Emotional Intelligence with Eye-Tracking Techniques
Authors: Wang Wei , Kofler Liat , Lindgren Chapman , Lobel Max , Murphy Amanda , Tong Qiwen , Pickering Kemar
Published: August 2023

AI, or artificial intelligence, is a technology of creating algorithms and computer systems that mimic human cognitive abilities to perform tasks. Many industries are undergoing revolutions due to the advances and applications of AI technology. The current study explored a burgeoning field—Psychometric AI, which integrates AI methodologies and psychological measurement to not only improve measurement accuracy, efficiency, and effectiveness but also help reduce human bias and increase objectivity in measurement. Specifically, by leveraging unobtrusive eye-tracking sensing techniques and performing 1470 runs with seven different machine-learning classifiers, the current study systematically examined the efficacy of various (ML) models in measuring different facets and measures of the emotional intelligence (EI) construct. Our results revealed an average accuracy ranging from 50–90%, largely depending on the percentile to dichotomize the EI scores. More importantly, our study found that AI algorithms were powerful enough to achieve high accuracy with as little as 5 or 2 s of eye-tracking data. The research also explored the effects of EI facets/measures on ML measurement accuracy and identified many eye-tracking features most predictive of EI scores. Both theoretical and practical implications are discussed.
A Benchmark for Maritime Object Detection with Centernet on an Improved Dataset, ABOships-PLUS
Authors: Iancu Bogdan , Winsten Jesper , Soloviev Valentin , Lilius Johan
Published: August 2023

Object detection from waterborne imagery is an essential aspect in maritime traffic management, navigation safety and coastal security. Building efficient autonomous systems, which can take decisions in critical situations, requires an integration of complex object detectors in real time. Object detectors trained on generic datasets often give unsatisfactory results in complex scenarios like the maritime environment, since only a fraction of their images contain maritime vessels. Publicly available domain-specific datasets are scarce, and they are limited in the number of images and annotations. Compared to object detection in applications such as autonomous vehicles, maritime vessel detection is considerably reduced in computer vision research. This creates a deficit in exhaustive benchmarking studies for maritime detection datasets. To bridge this gap, we relabel the ABOships dataset and benchmark a state-of-the-art center-based detector, Centernet, on the newly relabeled dataset, ABOships-PLUS. We explore its performance under different feature extractors, and investigate the effect of object size and inter-class variation on detection accuracy. The reported benchmarking illustrates that the ABOships-PLUS dataset is adequate to use in supervised domain adaptation. The experimental results show that Centernet with DLA (Deep Layer Aggregation) as a feature extractor achieved the highest accuracy in detecting maritime objects overall (with mean average precision 74.4%).
The Struggle for News Value in the Digital Era
Authors: Harcup Tony
Published: August 2023

News has long been a contested concept but in the digital era it has become increasingly fractured and multidimensional. This discursive article explores some of the ways in which the news has been disrupted by technological and economic tensions and argues that the social value of news is worth articulating and, where necessary, struggling for. News values have never been universal or unproblematic, and the tension between commercial and social ways of valuing news is intensified today. News values are not fixed and must be open to critique as to how they are meeting citizens’ needs. Societally useful news may be at risk of being marginalized as news organizations struggle to survive, but it is not inevitable that disruption and digitization should undermine journalistic ethics and the social value of news. In arguing that scholars ought to approach news more holistically, to defend it as well as critique it, the article attempts to synthesize what typically appear as discrete approaches to studying news. The article concludes that, if the social value of news is not to suffer further diminution, there is a need to view news through a lens of struggle; a struggle in which journalists, audiences, scholars and, indeed, all citizens have a part to play.
Research on Vegetation Ecological Security in Arid Region Mountain Front River Valleys Based on Ecological Water Consumption and Water Demand
Authors: Dong Xiangshou , Hu Shihang , Yuan Quanzhi , Kou Yaowen , Li Shujun , Deng Wei , Ren Ping
Published: August 2023

The central region of the Eurasian continent is widely affected by arid conditions, but the valleys in front of the mountains nurture ecosystems consisting of forests, shrubs, and grasslands. Preserving the ecological balance in these arid valley areas is an essential aspect of water resource planning and management. This study utilizes calculations of vegetation’s ecological water consumption and water requirements to quantitatively simulate groundwater levels. These simulated levels are then compared with the threshold depth suitable for vegetation, ultimately leading to the development of an ecological security assessment method for valley areas. The results show the following: (1) During 30 years, the water demand of river valley vegetation increased slowly, and the overall stability is about 4.82 × 108 m3. Among them, the ecological water demand of grassland is the largest. The water demand from June to August is about 68% of the whole year. (2) The results indicate that over a period of 30 years, the groundwater levels in the valley area have shown a gradual decline. The rate of decline in groundwater levels is approximately twice as fast in areas farther away from the river compared to areas closer to the river. The decline in groundwater levels typically begins in May each year. During the period of valley flooding in June, there is a temporary rise in water levels, followed by a continued decline afterwards. (3) The study area has a significant proportion of groundwater suitable areas, accounting for approximately 65% on average annually. Over the course of 30 years, the area experiencing groundwater deficiency has increased from 31% to 37%. (4) Over the past 30 years, the ratio of annual vegetation water consumption to water demand in the river valley has been slowly decreasing, and the vegetation growth status has changed from good growth to normal growth. (5) In the past 30 years, the area of ecological quality areas has decreased significantly, and most of them have been transformed into general areas. The area of ecologically fragile areas is increasing, and the area of fencing protected areas is slowly declining.
Spatial Properties of Soil Physical Quality Index S in Black Soil Croplands under Permanent Gully Erosion
Authors: He Yangbo , Song Xingsheng , Li Xinyue , Gao Yuhao , Yang Jingde , Chen Junxi , Chen Jiazhou , Cai Chongfa
Published: August 2023

Soil physical quality (SPQ) is a limiting factor affecting crop production. However, the impact of gully erosion on the SPQ index S, defined by Dexter as the inflection point of the soil water retention curve (SWRC), remains unclear, especially when considering different latitudinal regions. This study aimed to apply Dexter’s S-theory to evaluate the distribution of index S in black soils adjacent to various gully positions and investigate its relationship with bulk density (Bd), soil organic matter (SOM), and particle percentage. Soil properties (SWRC, Bd, SOM, and particle percentage) from nine gullies in croplands in three latitudinal regions (Harbin, Hailun, and Nenjiang in Heilongjiang province) were determined at the gully edge (GE0) and 50 m beyond the edge into the croplands (GE50) at the following gully units: head, mid-upper, middle, mid-lower, tail, and conjunctions between main gully and gully branch. The S-index was calculated using parameters such as n, θs, and θr, with SWRC data fitted into the van Genuchten model. The results showed spatial variations in the S-index across latitudinal regions, with slightly higher S-values in Harbin than in Hailun and Nenjiang. The S-index also showed noticeable differences at GE0 and GE50 and at the junctions between the main gully and its branches. Approximately 51% of the samples at GE0 and 28.2% of the samples at GE50 had S-values below 0.035, which Dexter proposed as the boundary between good and poor SPQ, indicating a degradation of SPQ at the gully-surrounding areas. A decreased S-index in the gully vicinity was significantly (p < 0.05) associated with increased bulk density (1.33 vs. 1.21 g cm−3 for GE0 and GE50) and decreased SOM (36.80 vs. 39.36 g kg−1 for GE0 and GE50). In summary, this study indicates that gully erosion affects the farmland S-index at the gully-surrounding areas through SOM and Bd. Accordingly, measures suited to the increase in the S-index of the gully-surrounding areas may be implemented to maximize the crop yield of farmlands.
Impact of Land Cover on Wind Erosion in Arid Regions: A Case Study in Southern Tunisia
Authors: Labiadh Mohamed Taieb , Rajot Jean Louis , Sekrafi Saâd , Ltifi Mohsen , Attoui Badie , Tlili Abderrazak , Hlel Moustapha , Bergametti Gilles , des Tureaux Thierry Henry , Bouet Christel
Published: August 2023

Wind erosion is a worldwide phenomenon for which several recent studies have shown that the intensity is expected to evolve because of climate and land use changes. Identifying the areas where wind erosion is the most active and its associated drivers may help to define efficient solutions to protect the environment from this hazard. The south of Tunisia is a region highly prone to wind erosion and presents a variety of landscapes and land uses in a relatively small area. Thus, from November 2012 to June 2016, six sites were instrumented to monitor wind erosion in the most extended land uses existing in southern Tunisia. The main results are that wind erosion: (i) is nil in the oasis, (ii) is weak (<120 kg m−1 yr−1) in the olive grove even if the surface is be ploughed up to eight times a year, (iii) is moderate (~1000 kg m−1 yr−1) in the barley field, for which the sowing date is a driving parameter of wind erosion seasonality, (iv) can occur in the Chott El Jerid except when it is flooded, (v) is the most intense in rangelands on sand (~2500 kg m−1 yr−1) and on flatbeds (>3000 kg m−1 yr−1).
Role of Functional Neuroimaging with 123I-MIBG and 123I-FP-CIT in De Novo Parkinson’s Disease: A Multicenter Study
Authors: De Feo Maria Silvia , Frantellizzi Viviana , Locuratolo Nicoletta , Di Rocco Arianna , Farcomeni Alessio , Pauletti Caterina , Marongiu Andrea , Lazri Julia , Nuvoli Susanna , Fattapposta Francesco , De Vincentis Giuseppe , Spanu Angela
Published: August 2023

Background: Parkinson’s disease is a progressive neurodegenerative disorder, with incidence and prevalence rates of 8–18 per 100,000 people per year and 0.3–1%, respectively. As parkinsonian symptoms do not appear until approximately 50–60% of the nigral DA-releasing neurons have been lost, the impact of routine structural imaging findings is minimal at early stages, making Parkinson’s disease an ideal condition for the application of functional imaging techniques. The aim of this multicenter study is to assess whether 123I-FP-CIT (DAT-SPECT), 123I-MIBG (mIBG-scintigraphy) or an association of both exams presents the highest diagnostic accuracy in de novo PD patients. Methods: 288 consecutive patients with suspected diagnoses of Parkinson’s disease or non- Parkinson’s disease syndromes were analyzed in the present Italian multicenter retrospective study. All subjects were de novo, drug-naive patients and met the inclusion criteria of having undergone both DAT-SPECT and mIBG-scintigraphy within one month of each other. Results: The univariate analysis including age and both mIBG-SPECT and DAT-SPECT parameters showed that the only significant values for predicting Parkinson’s disease in our population were eH/M, lH/M, ESS and LSS obtained from mIBG-scintigraphy (p < 0.001). Conclusions: mIBG-scintigraphy shows higher diagnostic accuracy in de novo Parkinson’s disease patients than DAT-SPECT, so given the superiority of the MIBG study, the combined use of both exams does not appear to be mandatory in the early phase of Parkinson’s disease.
Challenges towards the Sustainability and Enhancement of the Indian Sundarban Mangrove’s Blue Carbon Stock
Authors: Chanda Abhra , Akhand Anirban
Published: August 2023

The Sundarban is the world’s largest contiguous mangrove forest and stores around 26.62 Tg of blue carbon. The present study reviewed the factors causing a decline in its blue carbon content and poses a challenge in enhancing the carbon stock of this region. This review emphasized that recurrent tropical cyclones, soil erosion, freshwater scarcity, reduced sediment load into the delta, nutrient deficiency, salt-stress-induced changes in species composition, mangrove clearing, and anthropogenic pollution are the fundamental drivers which can potentially reduce the total blue carbon stock of this region. The southern end of the Ganges–Brahmaputra–Meghna Delta that shelters this forest has stopped its natural progradation due to inadequate sediment flow from the upper reaches. Growing population pressure from the north of the Sundarban Biosphere Reserve and severe erosion in the southern end accentuated by regional sea-level rise has left minimal options to enhance the blue carbon stock by extending the forest premises. This study collated the scholarly observations of the past decades from this region, indicating a carbon sequestration potential deterioration. By collecting the existing knowledge base, this review indicated the aspects that require immediate attention to stop this ecosystem’s draining of the valuable carbon sequestered and, at the same time, enhance the carbon stock, if possible. This review provided some key recommendations that can help sustain the blue carbon stock of the Indian Sundarban. This review stressed that characterizing the spatial variability of blue carbon with more sampling points, catering to the damaged trees after tropical cyclones, estuarine rejuvenation in the upper reaches, maintaining species diversity through afforestation programs, arresting coastal erosion through increasing sediment flow, and combating marine pollution have become urgent needs of the hour. The observations synthesized in this study can be helpful for academics, policy managers, and decision makers willing to uphold the sustainability of the blue carbon stock of this crucial ecosystem.
Identification and Functional Characterization of Mutation in FYCO1 in Families with Congenital Cataract
Authors: Ullah Muhammad Ikram , Rehman Zaira , Dad Rubina , Alsrhani Abdullah , Shakil Muhammad , Ghanem Heba Bassiony , Alameen Ayman Ali Mohammed , Elsadek Mohamed Farouk , Eltayeb Lienda Bashier , Ullah Sajjad , Atif Muhammad
Published: August 2023

Congenital cataract (CC) causes a third of the cases of treatable childhood blindness worldwide. CC is a disorder of the crystalline lens which is established as clinically divergent and has complex heterogeneity. This study aimed to determine the genetic basis of CC. Whole blood was obtained from four consanguineous families with CC. Genomic DNA was extracted from the blood, and the combination of targeted and Sanger sequencing was used to identify the causative gene. The mutations detected were analyzed in silico for structural and protein–protein interactions to predict their impact on protein activities. The sequencing found a known FYCO1 mutation (c.2206C>T; p.Gln736Term) in autosomal recessive mode in families with CC. Co-segregation analysis showed affected individuals as homozygous and carriers as heterozygous for the mutation and the unaffected as wild-type. Bioinformatics tools uncovered the loss of the Znf domain and structural compactness of the mutant protein. In conclusion, a previously reported nonsense mutation was identified in four consanguineous families with CC. Structural analysis predicted the protein as disordered and coordinated with other structural proteins. The autophagy process was found to be significant for the development of the lens and maintenance of its transparency. The identification of these markers expands the scientific knowledge of CC; the future goal should be to understand the mechanism of disease severity. Ascertaining the genetic etiology of CC in a family member facilitates establishing a molecular diagnosis, unlocks the prospect of prenatal diagnosis in pregnancies, and guides the successive generations by genetic counseling.
Recent Advances in Intraoperative Brainstem Auditory Evoked Potential Monitoring during Microvascular Decompression Surgery for Hemifacial Spasm
Authors: Park Sang-Ku , Lee Hyun Seok , Cho Kyung Rae , Park Kwan
Published: August 2023

Brainstem auditory evoked potential (BAEP) testing during microvascular decompression (MVD) is very important in the treatment of hemifacial spasm (HFS). The reason for this is that the vestibulocochlear nerve is located immediately next to the facial nerve, so the vestibulocochlear nerve may be affected by manipulation during surgery. BAEP testing for detecting vestibulocochlear nerve damage has been further developed for use during surgery. In most HFS patients with normal vestibulocochlear nerves, the degree of vestibulocochlear nerve damage caused by surgery is well-reflected in the BAEP test waveforms. Therefore, real-time testing is the best way to minimize damage to the vestibulocochlear nerve. The purpose of this study was to review the most recently published BAEP test waveforms that were obtained during MVD surgery to determine the relationship between vestibulocochlear nerve damage and BAEP waveforms.
Age and Sex-Related Differences in Teicoplanine Isoform Concentrations in SARS-CoV-2 Patients
Authors: Allegra Sarah , Chiara Francesco , Zanatta Marina , Mengozzi Giulio , Puccinelli Maria Paola , De Francia Silvia
Published: August 2023

Teicoplanin, a glycopeptide antibiotic commonly used to treat bacterial infections, was discovered to be active in vitro against SARS-CoV-2. The aim of this study was to assess the levels of teicoplanin and its components in a cohort of adult and pediatric SARS-CoV-2 patients, evaluating the effect of sex and age on analyte concentrations. The levels of AST, ALT and leukocytes were shown to be higher in females, while the C reactive protein was higher in males. Evaluating the absence/presence of teicoplanin isoforms, we observed that A2-2_3 is the only one consistently present in pediatrics and adults. In adult men and all pediatrics, A2-4_5 is always present. In pediatrics, except for A3-1, median isoform concentrations were higher in females; on the contrary, in adult patients, males showed higher levels. This is the first study to describe levels of teicoplanin isoforms in SARS-CoV-2 infected patients in males and females, and pediatrics and adults, despite the small sample size of our cohort. The observed results imply that additional testing, via therapeutic drug monitoring, may be helpful to more effectively manage infections, particularly those caused by the most recent viruses.
Frictional Characteristic Curves of Ground Surfaces in Lubricated Sliding
Authors: Parameswaran Gopakumar , Jayaram Vikram , Kailas Satish V.
Published: August 2023

The key objective of the presented study was to use a commercially feasible and scalable approach to modifying surfaces to reduce friction. In an industrial setting, surface grinding is commercially viable and scalable as compared to other surface modifying processes like laser surface texturing, plasma, or ion beam milling. Frictional force plots are generated from the lubricated contact interface between a flat-faced aluminum pin and a reciprocating stainless steel countersurface driven by a scotch yoke follower mechanism. Using a surface grinder and selecting coated abrasive sheets, different stainless steel surface specimens, classified as P320, P1200, and mirror were prepared and tested in this study. The frictional force encountered by the pin was recorded using a data acquisition system at discrete intervals in the reciprocating path and averaged along the sliding cycles. The shape of the frictional force plots thus generated were found to be different from each other. Various mechanisms of friction prevalent at the contact were presumed to influence the shape of these frictional plots. These mechanisms were tested by varying the sliding speeds, lubricating oil viscosities, and using tribofilm-forming additives. We used Group 1 base oil of two different viscosities in our tests. At lower speeds, the frictional force plot for the mirror-finished surface seemed to conform to the Stribeck curve, while in the same scale of reference, the P1200 surface had a force plot that was nearly flat and of very low magnitude. At the contact interface, there seemed to be a fine balance existing between adhesion and abrasion phenomena, while oil retention was promoted to achieve extremely low sliding friction.
The Lymphatic System—A Surgeon’s Point of View
Authors: Almeida Alexandre , Ofir Hagit , Zeltzer Assaf A.
Published: August 2023

Lymphedema is a chronic and debilitating disease that affects up to 250 million patients worldwide. Recent advances in understanding its pathophysiology, along with improved diagnosis and microsurgical techniques, have enhanced our ability to cope with the challenging task of treating this disease. This review provides an overview of the disease from a surgeon’s point of view, including existing imaging modalities used for preoperative assessment, as well as surgical procedures used in its treatment. The advantages and drawbacks of various existing modalities used for the pre- or intraoperative assessment of lymphatic vessels are discussed. Lymphedema treatment has shifted from palliative debulking procedures (liposuction and direct excision) to those aimed at restoring lymphatic flow and countering the pathophysiology of the disease (lymphaticovenous anastomosis and vascularized lymph node transfer). A combination of both approaches can result in a synergistic benefit for patients and is discussed in this review. Despite recent advances, some controversies persist, and further studies are needed to better define surgical treatment algorithms.
Nitrate-Nitrogen Adsorption Characteristics and Mechanisms of Various Garden Waste Biochars
Authors: Yao Jingjing , Wang Zhiyi , Liu Mengfan , Bai Bing , Zhang Chengliang
Published: August 2023

Nitrate-nitrogen (NO3−–N) removal and garden waste disposal are critical concerns in urban environmental protection. In this study, biochars were produced by pyrolyzing various garden waste materials, including grass clippings (GC), Rosa chinensis Jacq. branches (RC), Prunus persica branches (PP), Armeniaca vulgaris Lam. branches (AV), Morus alba Linn. sp. branches (MA), Platycladus orientalis (L.) Franco branches (PO), Pinus tabuliformis Carrière branches (PT), and Sophorajaponica Linn. branches (SL) at three different temperatures (300 °C, 500 °C, and 700 °C). These biochars, labeled as GC300, GC500, GC700, and so on., were then used to adsorb NO3−–N under various conditions, such as initial pH value, contact time, initial NO3−–N concentration, and biochar dosage. Kinetic data were analyzed by pseudo-first-order and pseudo-second-order kinetic models. The equilibrium adsorption data were evaluated by Langmuir, Freundlich, Temkin and Dubinin–Radushkevich models. The results revealed that the biochar yields varied between 14.43% (PT700) and 47.09% (AV300) and were significantly influenced by the type of garden waste and decreased with increasing pyrolysis temperature, while the pH and ash content showed an opposite trend (p < 0.05). The efficiency of NO3−–N removal was significantly influenced by the type of feedstock, preparation process, and adsorption conditions. Higher pH values had a negative influence on NO3−–N adsorption, while longer contact time, higher initial concentration of NO3−–N, and increased biochar dosage positively affected NO3−–N adsorption. Most of the kinetic data were better fitted to the pseudo-second-order kinetic model (0.998 > R2 > 0.927). Positive b values obtained from the Temkin model indicated an exothermic process of NO3−–N adsorption. The Langmuir model provided better fits for more equilibrium adsorption data than the Freundlich model, with the maximum NO3−–N removal efficiency (62.11%) and adsorption capacity (1.339 mg·g−1) in PO700 under the conditions of pH = 2, biochar dosage = 50 mg·L−1, and a reaction time of 24 h. The outcomes of this study contribute valuable insights into garden waste disposal and NO3−–N removal from wastewater, providing a theoretical basis for sustainable environmental management practices.