bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-environment evaluation of rice genotypes: impact of weather and culm biochemical parameters against sheath blight infection
Authors: Panda Siddharth , R. Naveen kumar , Pavani S. Lalitha , Ganesan Sangeetha , Singh Pawan Kumar , Sah Rameswar Prasad , V. Padmakumar , Subudhi Hatanath , Mahender Anumalla , Anandan Annamalai , Ali Jauhar
Published: October 2023

Introduction

Sheath blight caused by Rhizoctonia solani is one of the major diseases of rice, causing widespread crop losses. The use of semi-dwarf rice varieties in the ongoing nutrient-intensive rice cultivation system has further accentuated the incidence of the disease. An ideal solution to this problem would be identifying a stable sheath blight-tolerant genotype.

Material and methods

A multi-environment evaluation of 32 rice genotypes against sheath blight infection was conducted over six seasons across two locations (Agricultural Research Farm, Institute of Agricultural Sciences, Banaras Hindu University (28.18° N, 38.03° E, and 75.5 masl), for four years during the wet seasons (kharif) from 2015 to 2018 and two seasons at the National Rice Research Institute (20°27’09” N, 85°55’57” E, 26 masl), Cuttack, Odisha, during the dry season (rabi) of 2019 and the kharif of 2019, including susceptible and resistant check. Percent disease index data were collected over 4 weeks (on the 7th, 14th, 21st, and 28th day after infection), along with data on other morphological and physiological traits.

Result and discussion

The resistant genotypes across seasons were the ones with a higher hemicellulose content (13.93-14.64) and lower nitrogen content (1.10- 1.31) compared with the susceptible check Tapaswini (G32) (hemicellulose 12.96, nitrogen 1.38), which might explain the resistant reaction. Three different stability models—additive main effect and multiplicative interaction (AMMI), genotype + genotype x environment (GGE) biplot, and multi-trait stability index (MTSI)—were then used to identify the stable resistant genotypes across six seasons. The results obtained with all three models had common genotypes highlighted as stable and having a low area under the disease progress curve (AUDPC) values. The ideal stable genotypes with low disease incidence were IC 283139 (G19), Tetep (G28), IC 260917 (G4), and IC 277274 (G10), with AUDPC values of 658.91, 607.46, 479.69, and 547.94, respectively. Weather parameters such as temperature, rainfall, sunshine hours, and relative humidity were also noted daily. Relative humidity was positively correlated with the percent disease index.
Multi-trait selection in multi-environments for performance and stability in cassava genotypes
Authors: Sampaio Filho Juraci Souza , Olivoto Tiago , Campos Marcos de Souza , Oliveira Eder Jorge de
Published: October 2023

Genotype-environment interaction (GEI) presents challenges when aiming to select optimal cassava genotypes, often due to biased genetic estimates. Various strategies have been proposed to address the need for simultaneous improvements in multiple traits, while accounting for performance and yield stability. Among these methods are mean performance and stability (MPS) and the multi-trait mean performance and stability index (MTMPS), both utilizing linear mixed models. This study’s objective was to assess genetic variation and GEI effects on fresh root yield (FRY), along with three primary and three secondary traits. A comprehensive evaluation of 22 genotypes was conducted using a randomized complete block design with three replicates across 47 distinct environments (year x location) in Brazil. The broad-sense heritability (H2) averaged 0.37 for primary traits and 0.44 for secondary traits, with plot-based heritability (hmɡ2) consistently exceeding 0.90 for all traits. The high extent of GEI variance (σɡxe2) demonstrates the GEI effect on the expression of these traits. The dominant analytic factor (FA3) accounted for over 85% of the total variance, and the communality (ɧ) surpassed 87% for all traits. These values collectively suggest a substantial capacity for genetic variance explanation. In Cluster 1, composed of remarkably productive and stable genotypes for primary traits, genotypes BRS Novo Horizonte and BR11-34-69 emerged as prime candidates for FRY enhancement, while BRS Novo Horizonte and BR12-107-002 were indicated for optimizing dry matter content. Moreover, MTMPS, employing a selection intensity of 30%, identified seven genotypes distinguished by heightened stability. This selection encompassed innovative genotypes chosen based on regression variance index (Sdi2, R2, and RMSE) considerations for multiple traits. In essence, incorporating methodologies that account for stability and productive performance can significantly bolster the credibility of recommendations for novel cassava cultivars.
Engineering custom morpho- and chemotypes of Populus for sustainable production of biofuels, bioproducts, and biomaterials
Authors: Buell C. Robin , Dardick Christopher , Parrott Wayne , Schmitz Robert J. , Shih Patrick M. , Tsai Chung-Jui , Urbanowicz Breeanna
Published: October 2023

Humans have been modifying plant traits for thousands of years, first through selection (i.e., domestication) then modern breeding, and in the last 30 years, through biotechnology. These modifications have resulted in increased yield, more efficient agronomic practices, and enhanced quality traits. Precision knowledge of gene regulation and function through high-resolution single-cell omics technologies, coupled with the ability to engineer plant genomes at the DNA sequence, chromatin accessibility, and gene expression levels, can enable engineering of complex and complementary traits at the biosystem level. Populus spp., the primary genetic model system for woody perennials, are among the fastest growing trees in temperate zones and are important for both carbon sequestration and global carbon cycling. Ample genomic and transcriptomic resources for poplar are available including emerging single-cell omics datasets. To expand use of poplar outside of valorization of woody biomass, chassis with novel morphotypes in which stem branching and tree height are modified can be fabricated thereby leading to trees with altered leaf to wood ratios. These morphotypes can then be engineered into customized chemotypes that produce high value biofuels, bioproducts, and biomaterials not only in specific organs but also in a cell-type-specific manner. For example, the recent discovery of triterpene production in poplar leaf trichomes can be exploited using cell-type specific regulatory sequences to synthesize high value terpenes such as the jet fuel precursor bisabolene specifically in the trichomes. By spatially and temporally controlling expression, not only can pools of abundant precursors be exploited but engineered molecules can be sequestered in discrete cell structures in the leaf. The structural diversity of the hemicellulose xylan is a barrier to fully utilizing lignocellulose in biomaterial production and by leveraging cell-type-specific omics data, cell wall composition can be modified in a tailored and targeted specific manner to generate poplar wood with novel chemical features that are amenable for processing or advanced manufacturing. Precision engineering poplar as a multi-purpose sustainable feedstock highlights how genome engineering can be used to re-imagine a crop species.
The illusion of inclusion: contextual behavioral science and the Black community
Authors: Faber Sonya C. , Metzger Isha W. , La Torre Joseph , Fisher Carsten , Williams Monnica T.
Published: October 2023

Anti-racism approaches require an honest examination of cause, impact, and committed action to change, despite discomfort and without experiential avoidance. While contextual behavioral science (CBS) and third wave cognitive-behavioral modalities demonstrate efficacy among samples composed of primarily White individuals, data regarding their efficacy with people of color, and Black Americans in particular, is lacking. It is important to consider the possible effects of racial stress and trauma on Black clients, and to tailor approaches and techniques grounded in CBS accordingly. We describe how CBS has not done enough to address the needs of Black American communities, using Acceptance and Commitment Therapy (ACT) and Functional Analytic Psychotherapy (FAP) as examples. We also provide examples at the level of research representation, organizational practices, and personal experiences to illuminate covert racist policy tools that maintain inequities. Towards eradicating existing racism in the field, we conclude with suggestions for researchers and leadership in professional psychological organizations.
Impaired olfactory function in bipolar disorder patients during acute episodes regardless of psychotic symptoms
Authors: Li Yingying , Yuan Huiqian , Liu Xianlin , Su Langjun , Yang Chunhong , Chen Chao , Li Chunyang
Published: October 2023

Background

The aim of this study was to analyze whether the presence of psychotic symptoms affects olfactory function in patients with bipolar disorder (BD). We also compared olfactory function between the period of episode and remission in patients with BD.

Methods

BD patients in the acute phase were tracked to the remission stage. The psychiatric symptoms and social function of the enrolled subjects were assessed using the Hamilton Rating Scale for Depression (HAMD), the Young Mania Rating Scale (YMRS), the Hamilton Rating Scale for Anxiety (HAMA), the Positive and Negative Syndrome Scale (PANSS) and the Global Assessment Function (GAF). Olfactory sensitivity (OS) and olfactory identification (OI) was assessed using the Sniffin’ Sticks test. Differences in OS and OI among the episodic group, the euthymic group, and the healthy control (HC) group were compared. According to whether BD is accompanied by psychotic symptoms, the OS and OI in the BD with psychotic symptoms group (P-BD), the BD without psychotic symptoms group (NP-BD), and the HC group were compared.

Results

The P-BD and NP-BD groups exhibited impaired OI compared with the HC group, but there was no significant difference in OI between the P-BD and NP-BD groups, or in OS among all three groups. All patients with episodic BD had significantly lower OS and OI compared with the HC group. OI in euthymic BD patients was still impaired; however, OS recovered, showing no significant difference compared with that in the HC group.

Conclusion

The results indicate that patients with episodic BD have impaired OS and OI, regardless of psychotic symptoms. OI may be a characteristic marker of BD, and OS may be a state marker that can be used to distinguish between episodic and euthymic BD.
Correlates of residents' enrolment intention toward inclusive commercial health insurance in China: involvement, perceived benefit, perceived sacrifice, and government participation
Authors: Shen Tianyi , Wang Yinuo , Xie Jinping , Han Xu , Shao Rong , Jiang Rong
Published: October 2023

Background

As an application of inclusive finance in health insurance, inclusive commercial health insurance (ICHI) is a new public-private partnership-based health insurance scheme and has been vigorously promoted by the Chinese government in recent years to develop China Multi-level Health Insurance System, a system that aims to seek a mix of public and private sources to provide more affordable financial protection to all levels of society in line with their needs. However, the overall enrolment of ICHI scheme is still at a low level, and little is known about what influences residents' enrolment intentions. The aim of this study was to examine the multidimensional factors influencing residents' behavioral intentions and to develop a multivariate conceptual model to explore the psychographic process in the formation of enrolment intention.

Methods

The empirical data used for model validation were obtained from a cross-sectional study conducted in Nanjing, China, a representative pilot city of ICHI scheme in 2022. Exploratory factor analysis, ANOVA, standard multiple regression, and hierarchical multiple regression were mainly employed for hypothesis testing.

Results

The findings revealed that involvement, perceived benefit, and perceived sacrifice are all crucial psychographic process factors in the formation of residents' enrolment intentions. Government participation positively moderates the influence path of “perceived benefit—enrolment intention” but negatively moderates the path of “perceived sacrifice—enrolment intention”. Moreover, it was discovered that perceived benefit mediates the effect of involvement on enrolment intention, while perceived sacrifice does not.

Conclusions

Improving residents' perceived benefit and involvement degree of the product, as well as reducing their perceived sacrifice, are both key to increasing their enrolment intentions. This study also points out that one of the main dilemmas in the current development of ICHI scheme is the low level of involvement among residents, and that optimizing the product design to make it more relevant to residents' lives is a more beneficial strategy to increase overall involvement.
An extraordinary wave of SARS-CoV2, the youngest and the least serious
Authors: Justribó Elena , Falcon Alberto , Ruiz-Vert Maria José , Porque Juliana , Gros Silvia , Yuguero Oriol
Published: October 2023

The second wave of SARS-CoV2 in our health region began at the end of June 2020 coinciding with the widespread decrease in cases in all areas of Catalonia. This resulted in the relaxation of measures that coincided with the end of the school year and the arrival of seasonal workers for the fruit-picking campaign. This led to a rapid increase in cases that culminated in perimeter confinement of the Segrià in July 2020, the only such measure imposed in the whole of Spain that month. This increase in cases was attributed to the influx of agricultural workers with a change of trend in the age of persons affected that had until then consisted mainly of older people. Simultaneously, in other areas of Catalonia the number of cases had dropped dramatically.
Psychological distress is affected by fear of COVID-19 via lifestyle disruption and leisure restriction among older adults in Japan: a cross-sectional study
Authors: Zenba Yosuke , Kobayashi Akihiro , Imai Tadanori
Published: October 2023

Introduction

Engaging in social activities is an essential component of a healthy lifestyle for community-dwelling older adults. Critically, as with past disasters, there is concern about the effects of long-term activity restrictions due to the coronavirus disease 2019 (COVID-19) pandemic on health of older adults. However, the precise associations between fear of COVID-19, lifestyle satisfaction, leisure activities, and psychological distress are unclear.

Objective

The purpose of this study was to comprehensively determine the associations between fear of COVID-19, lifestyle satisfaction, leisure engagement, and psychological distress among community-dwelling older adults in the context of the ongoing COVID-19 pandemic.

Materials and methods

A questionnaire survey administered by mail was conducted from October 1 to October 15, 2021. The questionnaire included the Fear of COVID-19 Scale, the Lifestyle Satisfaction Scale, the Leisure Activity Scale for Contemporary Older Adults, and the Kessler Psychological Distress Scale-6. Based on previous studies, we developed a hypothetical model for the association between fear of COVID-19, lifestyle satisfaction, leisure engagement, and psychological distress and performed structural equation modeling to assess the relationships between these variables.

Results

Participants included 301 Japanese citizens (23.6% male, 76.4% female), with a mean age of 76.7 ± 4.58 years. Goodness-of-fit from structural equation modeling was generally good. Analysis of standardized coefficients revealed a significant positive relationship between fear of COVID-19 and psychological distress (β = 0.33, p < 0.001) and lifestyle satisfaction and leisure activities (β = 0.35, p < 0.001). We further observed a significant negative relationship between fear of COVID-19 and lifestyle satisfaction (β = −0.23, p < 0.001) and between leisure activities and psychological distress (β = −0.33, p < 0.001).

Conclusion

Fear of COVID-19 is significantly associated with psychological distress, both directly and via its effects on lifestyle satisfaction and leisure activities. That is, not only did fear of COVID-19 directly impact psychological distress of participants, it also affected psychological distress through lifestyle disruption and leisure restriction. This results may be used to better understand how a national emergency that substantially restricts daily life, such as COVID-19 or an earthquake disaster, can affect the psychological health and wellbeing of older, community-dwelling adults.
Playing the pipes: acoustic sensing and machine learning for performance feedback during endotracheal intubation simulation
Authors: Steffensen Torjus L. , Bartnes Barge , Fuglstad Maja L. , Auflem Marius , Steinert Martin
Published: October 2023

Objective: In emergency medicine, airway management is a core skill that includes endotracheal intubation (ETI), a common technique that can result in ineffective ventilation and laryngotracheal injury if executed incorrectly. We present a method for automatically generating performance feedback during ETI simulator training, potentially augmenting training outcomes on robotic simulators.

Method: Electret microphones recorded ultrasonic echoes pulsed through the complex geometry of a simulated airway during ETI performed on a full-size patient simulator. As the endotracheal tube is inserted deeper and the cuff is inflated, the resulting changes in geometry are reflected in the recorded signal. We trained machine learning models to classify 240 intubations distributed equally between six conditions: three insertion depths and two cuff inflation states. The best performing models were cross validated in a leave-one-subject-out scheme.

Results: Best performance was achieved by transfer learning with a convolutional neural network pre-trained for sound classification, reaching global accuracy above 98% on 1-second-long audio test samples. A support vector machine trained on different features achieved a median accuracy of 85% on the full label set and 97% on a reduced label set of tube depth only.

Significance: This proof-of-concept study demonstrates a method of measuring qualitative performance criteria during simulated ETI in a relatively simple way that does not damage ecological validity of the simulated anatomy. As traditional sonar is hampered by geometrical complexity compounded by the introduced equipment in ETI, the accuracy of machine learning methods in this confined design space enables application in other invasive procedures. By enabling better interaction between the human user and the robotic simulator, this approach could improve training experiences and outcomes in medical simulation for ETI as well as many other invasive clinical procedures.
OSH related risks and opportunities for industrial human-robot interaction: results from literature and practice
Authors: Heinold Eva , Funk Miriam , Niehaus Susanne , Rosen Patricia H. , Wischniewski Sascha
Published: October 2023

Robotic systems are an integral component of today’s work place automation, especially in industrial settings. Due to technological advancements, we see new forms of human-robot interaction emerge which are related to different OSH risks and benefits. We present a multifaceted analysis of risks and opportunities regarding robotic systems in the context of task automation in the industrial sector. This includes the scientific perspective through literature review as well as the workers’ expectations in form of use case evaluations. Based on the results, with regards to human-centred workplace design and occupational safety and health (OSH), implications for the practical application are derived and presented. For the literature review a selected subset of papers from a systematic review was extracted. Five systematic reviews and meta-analysis (492 primary studies) focused on the topic of task automation via robotic systems and OSH. These were extracted and categorised into physical, psychosocial and organisational factors based on an OSH-factors framework for advanced robotics developed for the European Agency for Safety and Health at Work (EU-OSHA). To assess the workers’ perspective, 27 workers from three European manufacturing companies were asked about their expectations regarding benefits and challenges of robotic systems at their workplace. The answers were translated and categorised in accordance with the framework as well. The statements, both from literature and the survey were then analysed according to the qualitative content analysis, to gain additional insight into the underlying structure and trends in them. As a result, new categories were formed deductively. The analysis showed that the framework is capable to help categorise both findings from literature and worker survey into basic categories with good interrater reliability. Regarding the proposed subcategories however, it failed to reflect the complexity of the workers’ expectations. The results of the worker evaluation as well as literature findings both predominantly highlight the psychosocial impact these systems may have on workers. Organisational risks or changes are underrepresented in both groups. Workers’ initial expectations lean towards a positive impact.
Reimagining the United States organ procurement and transplant network
Authors: Perakslis Eric , McCourt Brian , Knechtle Stuart
Published: October 2023

The United States system of solid organ transplantation is overseen by the Organ Procurement Transplantation Network (OPTN). Recent announcements from the Health Resources and Services Administration (HRSA) indicate their clear intention to reform the system. We suggest that the original intention of the National Organ Transplant Act (NOTA) to require one entity to oversee transplantation is critical to integrate policy with the complex realities of organ procurement and transplantation practice. We suggest that a contemporary business platform model best captures the appropriate structure for coordinating organ transplantation, as the seamless exchange of organs between related groups is the essential function to facilitate. A business platform framework that includes public and private, academic and industry partners can best accomplish the important goal of equitable and efficient organ transplantation.
Absolute Quantification of Donor-Derived Cell-Free DNA in Pediatric and Adult Patients After Heart Transplantation: A Prospective Study
Authors: Böhmer Jens , Wasslavik Carina , Andersson Daniel , Ståhlberg Anders , Jonsson Marianne , Wåhlander Håkan , Karason Kristjan , Sunnegårdh Jan , Nilsson Staffan , Asp Julia , Dellgren Göran , Ricksten Anne
Published: October 2023

In this prospective study we investigated a cohort after heart transplantation with a novel PCR-based approach with focus on treated rejection. Blood samples were collected coincidentally to biopsies, and both absolute levels of dd-cfDNA and donor fraction were reported using digital PCR. 52 patients (11 children and 41 adults) were enrolled (NCT03477383, clinicaltrials.gov), and 557 plasma samples were analyzed. 13 treated rejection episodes >14 days after transplantation were observed in 7 patients. Donor fraction showed a median of 0.08% in the cohort and was significantly elevated during rejection (median 0.19%, p < 0.0001), using a cut-off of 0.1%, the sensitivity/specificity were 92%/56% (AUC ROC-curve: 0.78). Absolute levels of dd-cfDNA showed a median of 8.8 copies/mL and were significantly elevated during rejection (median 23, p = 0.0001). Using a cut-off of 7.5 copies/mL, the sensitivity/specificity were 92%/43% for donor fraction (AUC ROC-curve: 0.75). The results support the feasibility of this approach in analyzing dd-cfDNA after heart transplantation. The obtained values are well aligned with results from other trials. The possibility to quantify absolute levels adds important value to the differentiation between ongoing graft damage and quiescent situations.
Machine Learning-Based Approach for Predicting Diabetes Employing Socio-Demographic Characteristics
Authors: Rahman Md. Ashikur , Abdulrazak Lway Faisal , Ali Md. Mamun , Mahmud Imran , Ahmed Kawsar , Bui Francis M.
Published: October 2023

Diabetes is one of the fatal diseases that play a vital role in the growth of other diseases in the human body. From a clinical perspective, the most significant approach to mitigating the effects of diabetes is early-stage control and management, with the aim of a potential cure. However, lack of awareness and expensive clinical tests are the primary reasons why clinical diagnosis and preventive measures are neglected in lower-income countries like Bangladesh, Pakistan, and India. From this perspective, this study aims to build an automated machine learning (ML) model, which will predict diabetes at an early stage using socio-demographic characteristics rather than clinical attributes, due to the fact that clinical features are not always accessible to all people from lower-income countries. To find the best fit of the supervised ML classifier of the model, we applied six classification algorithms and found that RF outperformed with an accuracy of 99.36%. In addition, the most significant risk factors were found based on the SHAP value by all the applied classifiers. This study reveals that polyuria, polydipsia, and delayed healing are the most significant risk factors for developing diabetes. The findings indicate that the proposed model is highly capable of predicting diabetes in the early stages.
Noise Prediction and Plasma-Based Control of Cavity Flows at a High Mach Number
Authors: Cai Hongming , Zhang Zhuoran , Li Ziqi , Li Hongda
Published: October 2023

Cavity flows are a prevalent phenomenon in aerospace engineering, known for their intricate structures and substantial pressure fluctuations arising from interactions among vortices. The primary objective of this research is to predict noise levels in high-speed cavity flows at Mach 4 for a rectangular cavity characterized by an aspect ratio of L/D = 7. Moreover, this study delves into the influence of the plasma actuator on noise control within the cavity flow regime. To comprehensively analyze acoustic characteristics and explore effective noise reduction strategies, a computational fluid dynamics technique with the combination of a delayed detached eddy simulation (DDES) and plasma phenomenological model is established. Remarkably, the calculated overall sound pressure level (OASPL) and plasma-induced velocity closely align with the experimental data, validating the reliability of the proposed approach. The results show that the dielectric barrier discharge (DBD) plasma actuator changes the movement range of a dominating vortex in the cavity to affect the OASPL at the point with the maximum noise level. The control of excitation voltage can reduce the cavity noise by 2.27 dB at most, while control of the excitation frequency can only reduce the cavity noise by 0.336 dB at most. Additionally, the increase in excitation frequency may result in high-frequency sound pressure, but the influence is weakened with the increase in the excitation frequency. The findings highlight the potential of the plasma actuator in reducing high-Mach-number cavity noise.
Electromagnetic Performance Analysis of Inhomogeneous Radome Walls Considering Temperature and Ablation
Authors: Dan Yihua , Yang Jian
Published: October 2023

The radome is an important component of the aircraft seeker. The radome of high-speed aircraft usually has ablation, which affects the electromagnetic performance of the radome. Therefore, it is important to study the electromagnetic performance of the radome wall during high-temperature ablation. However, most existing studies mainly consider the influence of temperature and ignore structural changes caused by ablation. To solve the above problems, this paper studies the influence of ablation on the electromagnetic performance of inhomogeneous radome walls (IRW) by considering the structure parameters, thermal expansion characteristics, and dielectric parameters during the ablation. The two typical types of IRW design methods are analyzed, the parameters calculation method in the ablation process is proposed, and the influence of ablation on the two types of IRW is studied. The results give the electromagnetic changing characteristics of the IRW under different ablation conditions. The contribution of this work is to lay a solid theoretical foundation for improving the performance of the radome in the ablation process, which is of great significance.
Application of a Fusion Attention Mechanism-Based Model Combining Bidirectional Gated Recurrent Units and Recurrent Neural Networks in Soil Nutrient Content Estimation
Authors: Wang Huan , Zhang Lixin , Zhao Jiawei
Published: October 2023

Accurately estimating soil nutrient content, including soil organic matter (OM), nitrogen (N), phosphorus (P), and potassium (K) levels, is crucial for optimizing agricultural practices and ensuring sustainable crop production. This paper proposes a model based on a fusion attention mechanism that combines bidirectional gated recurrent units (BiGRU) and recurrent neural networks (RNN) to estimate soil nutrient content. The proposed model integrates the fused attention mechanism with BiGRU and RNN to enhance the accuracy and effectiveness of soil nutrient prediction. The fused attention mechanism captures key features in the input data, while the BiGRU architecture captures both forward and backward contextual information, enabling the model to capture long-term dependencies in the data. The results demonstrate that the proposed Att-BiGRU-RNN model outperforms other constructed models, exhibiting a higher prediction accuracy and robustness. The model shows good estimation capabilities for soil OM, N, P, and K with estimation accuracies (R2) of 0.959, 0.907, 0.921, and 0.914, respectively. The application of this model in soil nutrient estimation has the potential to optimize fertilizer management, enhance soil fertility, and ultimately improve crop yield. Further research can explore the applicability of this model in precision agriculture and sustainable soil management practices, benefiting the agricultural sector and contributing to food security and environmental sustainability.
Impact of Phosphogypsum Application on Fungal Community Structure and Soil Health in Saline–Alkali-Affected Paddy Fields
Authors: Lu Guanru , Feng Zhonghui , Xu Yang , Jin Yangyang , Zhang Guohui , Hu Jiafeng , Yu Tianhe , Wang Mingming , Liu Miao , Yang Haoyu , Li Weiqiang , Liang Zhengwei
Published: October 2023

Modifying saline–alkali soil is crucial for ensuring food security and expanding arable land. Microorganisms play a key role in driving various biochemical processes in agricultural ecosystems. However, limited information exists on the changes in the microbial community and soil structure in soda saline-alkali soil under modified conditions. In this study, we examined the changes in soil physicochemical properties of saline–alkali soil altered by rice planting alone and by combined application of phosphogypsum in the Songnen Plain. The results demonstrated that phosphogypsum significantly improved the soil’s physicochemical properties; it notably reduced salinity and alkalinity while enhancing nutrient structure. Additionally, the utilization efficiency of carbon (C), nitrogen (N), and phosphorus (P) increased. Fungal community diversity also significantly improved, influenced mainly by soil water content (SWC), total organic carbon (TOC), soil organic matter (SOM), total nitrogen (TN) and sodium ion (Na+). TOC, SOM, TN, ESP, and Na+ served as the primary drivers affecting the fungal community. Our findings indicate that combining rice planting with phosphogypsum application effectively modifies saline–alkali soil, regulates fungal community structure, and enhances long-term soil health. Furthermore, the beneficial effects of phosphogypsum on saline–alkali soil persist for persists for several years, largely owing to its role in promoting microbial community growth.
The Responses of Stem and Leaf Functional Traits of Medicago sativa and Bromus inermis to Different Mixed Planting Patterns
Authors: Miao Fuhong , Yu Xiaoxu , Tang Xinkai , Liu Xindi , Tang Wei , Zhao Yanhua , Yang Chao , Xu Yufang , Yang Guofeng , Sun Juan
Published: October 2023

This study investigated the differences in stem and leaf growth characteristics of Medicago sativa and Bromus inermis in the Jiaozhou region of China during 2019–2020 under three different planting modes of the two forages: monoculture, mixed species sowing in the same rows, and mixed species sowing in alternating rows. No special management of the experimental plots was carried out in this study to simulate as much as possible the growth of forages in their natural state. The stem and leaf characteristics influencing the dry matter weight were calculated using grey correlation. These characteristics included leaf length, leaf width, leaf thickness, leaf area, leaf fresh weight, stem length, stem diameter, stem fresh weight, stem–leaf ratio, fresh matter yield, dry matter yield, and protein yield of M. sativa and B. inermis under different sowing methods in different years. The results showed that the weight pattern of the characteristics affecting the yield of M. sativa and B. inermis production was leaf area > stem diameter > leaf length > stem length > leaf width > leaf thickness, leaf area > leaf length > stem length > leaf width > leaf thickness > stem diameter. Considering all the growth factors, the production capacity was ranked as mixed sowing in alternating rows > mixed sowing in same rows > monoculture. Thus, the suitable mode for M. sativa–B. inermis sowing was mixed sowing in alternating rows.
Comparison of Diploid and Triploid Atlantic Salmon (Salmo salar) Physiological Embryonic Development
Authors: Howard Callum , Taylor John F. , Migaud Herve , Gutierrez Alejandro P. , Bekaert Michaël
Published: October 2023

Diploid and triploid Atlantic salmon show distinct physiological differences including heart, brain, and digestive system morphology, propensity for certain deformities, temperature tolerance as eggs and once hatched, and different nutritional requirements. Whilst several studies have looked in detail at the rate of embryogenesis in diploid salmon, no study has compared the rate of embryogenesis between ploidies from fertilisation to hatch. This study based its assessment on a seminal paper by Gorodilov (1996) and used the same techniques to compare the rate at which triploid and diploid embryos developed morphological characteristics. Whilst no significant difference was found, this study provides well-needed justification for the assumption that both ploidies develop at the same rate and gives scientific weight to studies which involve manipulation at these stages of development. Two factors that did differ, however, were the timing of hatch, and mortality. Triploids hatched more quickly than diploids and reached 50% hatch at a significantly earlier point. Triploids also suffered from a significantly higher rate of mortality.
Phenotypic Characterization of Encephalitis in the BRAINS of Badgers Naturally Infected with Canine Distemper Virus
Authors: Espinoza Israel , García Iglesias María José , Oleaga Álvaro , de Garnica García María Gracia , Balseiro Ana
Published: October 2023

Canine distemper virus (CDV) affects a huge diversity of domestic and wild carnivores, with increasing numbers of mortality events worldwide. The local cell-mediated immune response elicited against a natural infection is an important factor in determining the outcome of CDV infection. Therefore, the purposes of this study were to describe the local immune response within the central nervous systems (CNSs) of seven badgers naturally infected with CDV in Asturias (Atlantic Spain) and to determine the phenotype and distribution of microglial cells, T and B lymphocytes, and astrocytes in the foci of gliosis located in the thalamus and cerebellum using immunohistochemistry. The immunohistochemical assessment demonstrated the presence of Iba1-positive microglia and GFAP-positive astrocytes in the foci of gliosis, whereas T (CD3-negative) or B (CD20-negative) lymphocytes in those same lesions were absent. Our results also revealed that the badgers with natural CDV encephalitis presented lesions mostly located in the white matter of the thalamus and cerebellum, suggesting a CDV-specific tropism for the white matter of badger brains in those locations. The knowledge gained in the field of the immunopathogenesis of distemper disease affecting the CNSs of badgers could help to clarify CDV disease patterns in this species.
Amphibians and Reptiles of the Montagne des Français: An Update of the Distribution and Regional Endemicity
Authors: Oninjatovo Radonirina Herizo , Randriamahatantsoa Bernard , Rabibisoa Nirhy H. C. , Raxworthy Christopher John
Published: October 2023

The harmonious landscape of Montagne des Français is a protected area in the far north of Madagascar. Our herpetofauna surveys were conducted on the eastern and western slopes according to habitat variations within the massif for 2014 and 2020. Our research updates the herpetofauna species richness, spatial distribution ranges, and ecological guilds within the protected area. We used direct opportunistic observations, systematic refuge examinations, and pitfall traps with drift fences at three sites to sample animals. Nineteen amphibian and fifty reptile species were recorded during this study. Three amphibian species and one reptile species, in addition to the snake Langaha pseudoalluaudi, last recorded in 2007, were discovered at Montagne des Français. Here, we present a database update for the herpetofauna species from Montagne des Français and provide a specific morphological description of the morphospecies considered as a new extension or localized distribution. This new database can help site managers develop new strategic conservation plans in response to habitat modification.
Wild Ungulates Constitute the Basis of the Diet of the Iberian Wolf in a Recently Recolonized Area: Wild Boar and Roe Deer as Key Species for Its Conservation
Authors: Barja Isabel , Navarro-Castilla Álvaro , Ortiz-Jiménez Lorena , España Ángel , Hinojosa Roberto , Sánchez-Sotomayor David , Iglesias Ángel , España José , Rubio-Sánchez Sergio , Martín-Romero Santiago , Vielva Juan , Horcajada-Sánchez Fernando
Published: October 2023

The Iberian wolf (Canis lupus signatus) is recolonizing historical distribution areas after decades of absence. As in other human-dominated landscapes, finding a balance to protect this species by favoring recolonization and mitigating human–wildlife conflicts is a challenge. Since wolves are often generalist opportunistic predators, we studied their diet composition in central Spain to evaluate the consumption of domestic ungulates and provide reliable data that could help local authorities to deal with the current wolf–cattle ranchers conflict and coexistence. Diet composition (% prey occurrence, % prey ingested biomass) was analyzed through the identification of prey hairs present in 671 scats collected between 2017 and 2021. The wolves fed more on wild ungulates (82% occurrence) than domestic ones (18%). Wild boar (Sus scrofa, 44% occurrence) and roe deer (Capreolus capreolus, 35%) were the most consumed prey. The wolves positively selected these two species. The wolves’ diets varied between seasons, years, and forest regions, but a diet based on wild ungulates predominated over domestic ones. Food niche breadth showed variations depending on seasons and years. Preserving the availability and diversity of wild ungulates may favor reducing livestock attacks and would be an achievable goal that would help to conserve this species and reduce conservation conflicts.
Effects of Different Galacto-Oligosaccharide Supplementation on Growth Performance, Immune Function, Serum Nutrients, and Appetite-Related Hormones in Holstein Calves
Authors: Yu Xin , Ma Fengtao , Dai Haonan , Liu Junhao , Hashem Nesrein M. , Sun Peng
Published: October 2023

Our previous study showed that early supplementation with 10 g/(d·head) of galacto-oligosaccharides (GOS) in newborn Holstein dairy calves reduced the incidence of diarrhea and improved growth performance and mineral absorption. Since the dose of 10 g/(d·head) was the lowest by dose screening in our previous study, the present study was designed to investigate whether a lower amount of GOS has similar effects on growth performance, immune function, serum nutrients in newborn Holstein heifer calves, and to further investigate its effect on appetite-related hormones. Twenty-eight healthy newborn (1 day of age) Holstein heifers with similar average body weight (41.18 ± 1.90 kg) were randomly divided into four groups (n = 7): the control group (CON group), which received heated raw milk, and three experimental groups, which received heated raw milk supplemented with 2.5 (GOS2.5 group), 5 (GOS5 group), and 10 g/(d·head) (GOS10 group) GOS. All heifer calves were fed the same starter for 28 d. Supplementation with GOS linearly increased the final body weight, average daily gain, and feed efficiency in heifer calves (p < 0.01). Compared with the control group, the average daily gain and feed efficiency of heifer calves were significantly higher in the GOS5 and GOS10 groups than in the control group (p < 0.05). Furthermore, supplementation with GOS quadratically enhanced the starter and total average daily feed intake of the heifers (p < 0.01), especially in the GOS2.5 and GOS5 groups, (p < 0.05 vs. CON). The serum concentration of immunoglobulin A was linearly increased by GOS supplementation (p < 0.05), and the levels in the GOS5 and GOS10 groups were significantly higher than those in the CON group. Meanwhile, GOS linearly decreased serum interleukin-1β and interleukin-6 concentrations (p < 0.05). The serum concentration of triglycerides was also linearly decreased (p < 0.05), whereas total protein and blood urea nitrogen were linearly increased (p < 0.05). Supplementation with GOS linearly decreased the serum concentration of leptin (p < 0.05) but increased cholecystokinin and glucagon-like peptide-1 (p < 0.05). Increasing doses of GOS linearly improved serum calcium and copper concentrations (p < 0.01) and quadratically enhanced the concentration of magnesium, which peaked in the GOS5 group (p < 0.05). In conclusion, GOS supplementation reduced the incidence of diarrhea and improved the growth performance and immune function of Holstein heifer calves.
Evaluation of the Role of Veterinarians for Outcomes Related to the Health and Production of Dairy Small Ruminants in Greece
Authors: Lianou Daphne T. , Fthenakis George C.
Published: October 2023

The objective of the present study was to evaluate the potential benefits of veterinarians in improving the health and welfare of dairy sheep and goats by studying the associations of management practices employed in the farms with production- or health-related outcomes in sheep and goat farms in Greece. This work explored associations with ‘professional relationship with a veterinarian’ at 444 small ruminant dairy farms in an investigation performed around Greece, where 106 variables, related to infrastructure, animals, production outcomes, health management, health problems and human resources, were assessed. In 384 (86.5%) farms, a professional relationship with a veterinarian was maintained. The median value of visits made annually by veterinarians to these farms was five. In farms with a professional relationship with a veterinarian, significant differences were found in 24 variables (35.8%) related to management practices and 6 (30.0%) production- or health-related outcomes. In multivariable analysis, the following emerged with a significant association: epg counts in faecal samples (p = 0.014), average annual milk production per ewe/doe (p = 0.015), somatic cell counts in bulk-tank milk (p = 0.037), and annual incidence of clinical mastitis (p = 0.044). Moreover, associations of the characteristics of veterinarians emerged with somatic cell counts in bulk-tank milk: the gender (p < 0.0001) and the age (p = 0.004) of the veterinarians. The results attest that the application of veterinary advice and clinical services in sheep and goat dairy farms contributes to the improved health, production and welfare of animals.
Study on Two-Dimensional Exit Pupil Expansion for Diffractive Waveguide Based on Holographic Volume Grating
Authors: Weng Mianhui , Wang Yizhao , Ren Xuechang , Lu Qinghong
Published: October 2023

Diffraction gratings are becoming a preferred option for waveguide head-mounted in–out coupling devices due to their flexible optical properties and small size and light weight. At present, diffraction waveguide coupling devices for AR head-mounted displays (HMD) have difficulties such as a long development cycle and complicated processing. In this paper, we first establish a set of two-dimensional (2D) grating ray tracing models, based on which we determine the initial architecture of the dual-region two-dimensional exit pupil expansion (2D-EPE) AR-HMD holographic waveguide diffraction system. Second, we propose a honeycomb coupled grating array and optimize the optical energy utilization and brightness uniformity of the holographic waveguide and use a custom dynamic linked library (DLL) function to implement the ray tracing of the 2D grating and add a probabilistic splitting function to the DLL, which reduces the single simulation time from 11.853 min to 1.77 min. We also propose a holographic lithography device composed of holographic optical elements (HOEs) and a method for preparing HOEs. Finally, in order to obtain the diffraction efficiency preoptimized by the above DLL for the uniformity of the exit pupil brightness and light energy utilization, we inverse design with the preparation process parameters as the optimization variables and develop the adaptable electromagnetic calculation program Holo-RCWA. Using Holo-RCWA with nondominated sorting genetic algorithm II (NSGA-II), we inverse design to obtain the process parameters satisfying the diffraction efficiency distribution, and the optimization time of the whole system is reduced from 2–3 days to 10 h. This work is of great significance for AR/VR applications.
Old Is the New: Immersive Explorations in Another Beautiful Country—Moving Images by Chinese American Artists
Authors: Lin Jenny
Published: October 2023

This article explores how diasporic Chinese video artists present familial histories and tales of cross-cultural exchange in the context of an exhibition I am curating, Another Beautiful Country: Moving Images by Chinese American Artists, at the University of Southern California (USC) Pacific Asia Museum. I discuss projects by featured artists Richard Fung and Patty Chang. These artists’ experimental documentaries and performative videos foster deep personal discoveries that defy the late-capitalist obsession with the new as defined by youth, novelty, and the next trend, providing revelatory insights through recuperative engagements with what has come before. In analyzing artworks by Fung and Chang, I also reference related texts by/about artists and historical figures including Walter Benjamin, Anna May Wong, and Zhang Ailing, who emigrated from the People’s Republic of China to the United States in the 1950s and whose special collections in the USC Libraries helped inform the exhibition’s programming. I also interweave my own related familial histories and share some (not-so-new) curatorial ideas for immersing audiences in intercultural art and reflection.