bluuebunny/crossref_metadata_2025 huggingface dataset:
Flexible terahertz gas sensing platform based on substrate-integrated hollow waveguides and an opto-electronic light source
Authors: Theiner Dominik , Limbacher Benedikt , Jaidl Michael , Ertl Marie , Hlavatsch Michael , Unterrainer Karl , Mizaikoff Boris , Darmo Juraj
Published: March 2023

We report on a flexible platform for molecular sensing in the terahertz range. Merging the well-established technologies of near-infrared electro-optic modulation and photomixing realizes a spectrally adaptable terahertz source, which is combined with a new generation of compact gas cells, so-called substrate-integrated hollow waveguides (iHWGs). iHWGs have been developed in the mid-infrared and provide flexibility in the design of the optical absorption path. Here, we demonstrate its suitability for the terahertz domain by presenting its low propagation losses and by measuring rotational transitions of nitrious oxide (N2O). A fast frequency sideband modulation technique results in substantially reduced measurement times and increased accuracy compared to a standard wavelength tuning method.
Spectrally flat mid-infrared supercontinuum pumped by a high power 2 µm noise-like pulse
Authors: Zhu Xiran , Zhao Desheng , Zhang Bin , Yang Linyong , Yao Jinmei , Yang Yukun , Liu Shuailin , Hou Jing
Published: March 2023

We demonstrate spectrally flat high-power mid-infrared supercontinuum (MIR SC) generation with record-breaking power of 33.1 W and power conversion efficiency of 75.06%. It is pumped by a 2 µm master oscillator power amplifier system consisting of a figure-8 mode-locked noise-like pulse seed laser and dual-stage Tm-doped fiber amplifiers with repetition rate of 4.08 MHz. Through cascading a piece of ZBLAN fiber with 13.5 µm large core diameter by direct-low-loss fusion splicing, SCs with spectral ranges of 1.9–3.68 µm, 1.9–3.84 µm, 1.9–4.02 µm and average powers of 33.1 W, 29.8 W, 25.9 W are generated. To the best of our knowledge, all of them have achieved the highest output power under the same condition of MIR spectrum range. This high-power all-fiber MIR SC laser system has relatively simple architecture, high efficiency and flat spectrum, demonstrating the advantages of 2 µm noise-like pulse pump in high-power MIR SC generation.
Polarisation compensation in non-planar image-rotating OPO ring resonators
Authors: Piotrowski Marcin , Schellhorn Martin , Spindler Gerhard , Medina Manuel A. , Müller Christian W. , Bigotta Stefano , Hildenbrand-Dhollande Anne
Published: March 2023

Non-planar image-rotating OPO ring resonators necessitate polarisation compensation in contrast to their planar counterparts. This is essential for maintaining phase matching conditions for non-linear optical conversion in the resonator during each cavity round trip. In this study, we examine the polarisation compensation and its impact on the performance of two types of non-planar resonators: RISTRA with a π2 image rotation and FIRE with a fractional image rotation (π2 fraction). The RISTRA is insensitive to mirror phase shifts, while the FIRE has a more complex dependence of polarisation rotation on mirror phase shifts. There has been debate over whether a single birefringent element can provide adequate polarisation compensation for non-planar resonators beyond RISTRA-type. Our results show that under certain experimentally feasible conditions, even FIRE resonators can achieve adequate polarisation compensation with a single half-wave plate. We validate our theoretical analysis through numerical simulations and experimental studies of OPO output beam polarisation using ZnGeP2 non-linear crystals.
Frequency-doubled Q-switched 4 × 4 multicore fiber laser system
Authors: Aleshire Christopher , Eichner Timo , Steinkopff Albrecht , Klenke Arno , Jauregui Cesar , Palmer Guido , Kuhn Stefan , nold Johannes , Haarlammert Nicoletta , Leemans Wim P. , Schreiber Thomas , Maier Andreas R. , Limpert Jens
Published: March 2023

Frequency doubling of a Q-switched Yb-doped rod-type 4 × 4 multicore fiber (MCF) laser system is reported. A second harmonic generation (SHG) efficiency of up to 52% was achieved with type I non-critically phase-matched lithium triborate (LBO), with a total SHG pulse energy of up to 17 mJ obtained at 1 kHz repetition rate. The dense parallel arrangement of amplifying cores into a shared pump cladding enables a significant increase in the energy capacity of active fibers. The frequency-doubled MCF architecture is compatible with high-repetition-rate and high-average-power operation and may provide an efficient alternative to bulk solid-state systems as pump sources for high-energy titanium-doped sapphire lasers.
High-Q Fano resonances in diamond nanopillars
Authors: Bonino Vittorio , Angelini Angelo
Published: March 2023

We report on the optical behaviour of a nanostructured diamond surface on a glass substrate. The numerical model reveals that a simple geometrical pattern sustains Fano-like resonances with a Q-factor as high as 3.5 · 105 that can be excited by plane waves impinging normally on the surface. We show that the geometrical parameters of the nanopillars affect both the resonant frequency and the line shape. The nanostructured surface can be straightforwardly used as a refractive index sensor with high sensitivity and linearity. Our findings show that diamond-based meta-surfaces are a valuable nanophotonic platform to control light propagation at the nanoscale, enabling large field enhancement within the nanoresonators that can foster both linear and nonlinear effects.
Expression of β-Catenin, E-Cadherin, and α-Smooth Muscle Actin in Basal Cell Carcinoma Before Photodynamic Therapy in Non-recurrent and Recurrent Tumors: Exploring the Ability of Predicting Photodynamic Therapy Outcome
Authors: Mørk Erik , Mjønes Patricia , Foss Olav A. , Bachmann Ingeborg M. , Christensen Eidi
Published: March 2023

Photodynamic therapy (PDT) is an effective and cosmetically beneficial treatment of low-risk basal cell carcinomas (BCCs). To optimize PDT response, it is important to correctly select tumors. We sought to find markers that could identify such tumors beyond contributions from clinical and histological examination. Studies have shown that β-catenin, E-cadherin, and α-smooth muscle actin (SMA) expression can indicate BCC aggressiveness/BCC invasiveness. We wanted to use these markers in an explorative study to investigate whether they were differently expressed among non-recurring compared with recurring BCCs, to evaluate their ability of predicting PDT outcome. Fifty-two BCCs were stained with antibodies against β-catenin, E-cadherin, and α-SMA, and evaluated using immunoreactive score (IRS), subcellular localization, and stromal protein expression. Results showed that IRS of E-cadherin was significantly different among recurring compared with non-recurring BCCs and with area under a receiver operating characteristic curve of 0.71 (95% confidence interval: 0.56–0.86, p=0.025). Stromal β-catenin expression significantly increased among recurring BCCs. Some recurring BCCs had intense expression in the deep invading tumor edge. In conclusion, E-cadherin, and stromal and deep edge β-catenin expression were most prominent in BCCs that recurred post-PDT, suggesting they could potentially predict PDT outcome. Further studies are needed to investigate whether these results are of clinical value:
Multiplex Immunofluorescence Image Quality Checking Using DAPI Channel–referenced Evaluation
Authors: Jiang Jun , Moore Raymond , Jordan Clarissa E. , Guo Ruifeng , Maus Rachel L. , Liu Hongfang , Goode Ellen , Markovic Svetomir N. , Wang Chen
Published: March 2023

Multiplex immunofluorescence (MxIF) images provide detailed information of cell composition and spatial context for biomedical research. However, compromised data quality could lead to research biases. Comprehensive image quality checking (QC) is essential for reliable downstream analysis. As a reliable and specific staining of cell nuclei, 4′,6-diamidino-2-phenylindole (DAPI) signals were used as references for tissue localization and auto-focusing across MxIF staining–scanning–bleaching iterations and could potentially be reused for QC. To confirm the feasibility of using DAPI as QC reference, pixel-level DAPI values were extracted to calculate signal fluctuations and tissue content similarities in staining–scanning–bleaching iterations for identifying quality issues. Concordance between automatic quantification and human experts’ annotations were evaluated on a data set consisting of 348 fields of view (FOVs) with 45 immune and tumor cell markers. Cell distribution differences between subsets of QC-pass vs QC-failed FOVs were compared to investigate the downstream effects. Results showed that 87.3% FOVs with tissue damage and 73.4% of artifacts were identified. QC-failed FOVs showed elevated regional gathering in cellular feature space compared with the QC-pass FOVs. Our results supported that DAPI signals could be used as references for MxIF image QC, and low-quality FOVs identified by our method must be cautiously considered for downstream analyses.
Topological descriptors of the parameter region of multistationarity: Deciding upon connectivity
Authors: Telek Máté László , Feliu Elisenda
Published: March 2023

Switch-like responses arising from bistability have been linked to cell signaling processes and memory. Revealing the shape and properties of the set of parameters that lead to bistability is necessary to understand the underlying biological mechanisms, but is a complex mathematical problem. We present an efficient approach to address a basic topological property of the parameter region of multistationary, namely whether it is connected. The connectivity of this region can be interpreted in terms of the biological mechanisms underlying bistability and the switch-like patterns that the system can create. We provide an algorithm to assert that the parameter region of multistationarity is connected, targeting reaction networks with mass-action kinetics. We show that this is the case for numerous relevant cell signaling motifs, previously described to exhibit bistability. The method relies on linear programming and bypasses the expensive computational cost of direct and generic approaches to study parametric polynomial systems. This characteristic makes it suitable for mass-screening of reaction networks. Although the algorithm can only be used to certify connectivity, we illustrate that the ideas behind the algorithm can be adapted on a case-by-case basis to also decide that the region is not connected. In particular, we show that for a motif displaying a phosphorylation cycle with allosteric enzyme regulation, the region of multistationarity has two distinct connected components, corresponding to two different, but symmetric, biological mechanisms.
A genome sequence for Biomphalaria pfeifferi, the major vector snail for the human-infecting parasite Schistosoma mansoni
Authors: Bu Lijing , Lu Lijun , Laidemitt Martina R. , Zhang Si-Ming , Mutuku Martin , Mkoji Gerald , Steinauer Michelle , Loker Eric S.
Published: March 2023

Background

Biomphalaria pfeifferiis the world’s most widely distributed and commonly implicated vector snail species for the causative agent of human intestinal schistosomiasis,Schistosoma mansoni. In efforts to controlS.mansonitransmission, chemotherapy alone has proven insufficient. New approaches to snail control offer a way forward, and possible genetic manipulations of snail vectors will require new tools. Towards this end, we here offer a diverse set of genomic resources for the important African schistosome vector,B.pfeifferi.

Methodology/Principal findings

Based largely on PacBio High-Fidelity long reads, we report a genome assembly size of 772 Mb forB.pfeifferi(Kenya), smaller in size than known genomes of other planorbid schistosome vectors. In a total of 505 scaffolds (N50 = 3.2Mb), 430 were assigned to 18 large linkage groups inferred to represent the 18 known chromosomes, based on whole genome comparisons withBiomphalaria glabrata. The annotatedB.pfeifferigenome reveals a divergence time of 3.01 million years withB.glabrata, a South American species believed to be similar to the progenitors ofB.pfeifferiwhich undertook a trans-Atlantic colonization < five million years ago.

Conclusions/Significance

The genome for this preferentially self-crossing species is less heterozygous than related species known to be preferential out-crossers; its smaller genome relative to congeners may similarly reflect its preference for selfing. Expansions of gene families with immune relevance are noted, including theFReDgene family which is far more similar in its composition toB.glabratathan toBulinus truncatus, a vector forSchistosoma haematobium. Provision of this annotated genome will help better understand the dependencies of trematodes on snails, enable broader comparative insights regarding factors contributing to susceptibility/ resistance of snails to schistosome infections, and provide an invaluable resource with respect to identifying and manipulating snail genes as potential targets for more specific snail control programs.
Knockout of ribosomal protein RpmJ leads to zinc resistance in Escherichia coli
Authors: Shirakawa Riko , Ishikawa Kazuya , Furuta Kazuyuki , Kaito Chikara
Published: March 2023

Zinc is an essential metal for cells, but excess amounts are toxic. Other than by regulating the intracellular zinc concentration by zinc uptake or efflux, the mechanisms underlying bacterial resistance to excess zinc are unknown. In the present study, we searched for zinc-resistant mutant strains from the Keio collection, a gene knockout library of Escherichia coli, a model gram-negative bacteria. We found that knockout mutant of RpmJ (L36), a 50S ribosomal protein, exhibited zinc resistance. The rpmJ mutant was sensitive to protein synthesis inhibitors and had altered translation fidelity, indicating ribosomal dysfunction. In the rpmJ mutant, the intracellular zinc concentration was decreased under excess zinc conditions. Knockout of ZntA, a zinc efflux pump, abolished the zinc-resistant phenotype of the rpmJ mutant. RNA sequence analysis revealed that the rpmJ mutant exhibited altered gene expression of diverse functional categories, including translation, energy metabolism, and stress response. These findings suggest that knocking out RpmJ alters gene expression patterns and causes zinc resistance by lowering the intracellular zinc concentration. Knockouts of other ribosomal proteins, including RplA, RpmE, RpmI, and RpsT, also led to a zinc-resistant phenotype, suggesting that deletion of ribosomal proteins is closely related to zinc resistance.
Adapting waterpipe-specific pictorial health warning labels to the Tunisian context using a mixed method approach
Authors: Ben Mansour Nadia , Rejaibi Salsabil , Sassi Mahfoudh Asma , Ben Youssef Sarra , Ben Romdhane Habiba , Schmidt Michael , D. Ward Kenneth , Maziak Wasim , Asfar Taghrid
Published: March 2023

Background

Waterpipe (WP) use is rapidly increasing among young people worldwide due to the widespread misperception that it is safer than cigarette smoking. Health warning labels (HWLs) can effectively communicate tobacco-related health risks but have yet to be developed for WP. This study aimed to optimize and adapt a set of 16 pictorial WP-specific HWLs, developed by an international Delphi study, to the Tunisian context. HWLs were grouped into four themes: WP health risks, WP harm to others, WP-specific harms, and WP harm compared to cigarettes.

Methods

Using a mixed method approach, we conducted ten focus groups combined with a survey among young WP users and nonusers (N = 63; age 18–34 years). In the survey, participants rated the HWLs on several communication outcomes (e.g., reaction, harm perception, effectiveness) and were then instructed to view all HWLs in each theme and rank them in the order of overall perceived effectiveness, from the most to the least effective. Afterward, participants provided in-depth feedback on HWLs and avenues for improvement. Mean effectiveness rating scores and percentages of participants’ top-ranked HWLs were calculated. Discussions were audio-taped, transcribed verbatim, and analyzed thematically.

Results

The top-ranked HWLs were those showing oral cancers, orally transmitted diseases, and a sick child. Focus group discussion illustrated that these selections were based on participants’ reactions to the direct impact of WP on a person’s physical appearance and evoking guilt over children’s exposure to WP smoke. Suggestions for improvement highlighted the need to use the local dialect and more affirmative statements (e.g., avoiding "may" or "can").

Conclusions

This study is the first in North Africa to attempt to advance HWLs policy as the World Health Organization recommended. The results of this study can be used as a basis for implementing WP-specific health messages in the Eastern Mediterranean Region.
Impact of new talent settlement policy on housing prices: Evidence from 70 large and medium-sized Chinese cities
Authors: Zhang Liguo , Li Ying , Kung Chih-Chun , Wu Bingcheng , Zhang Cheng
Published: March 2023

Since 2017, Chinese cities have set off a wave of talent migration, with major cities joining the talent war and issuing new talent settlement policies that might stimulate the real estate market through the inflow and outflow of human capital. However, the effects of new talent settlement policies on housing prices have not been extensively studied. This study used a difference-in-differences model to examine the causal effects of new talent settlement policies on housing prices in China based on data from 70 large and medium-sized cities. The results showed that new talent settlement policies had positive effects on housing prices, and the effects revealed pronounced regional heterogeneity: they were more significant in the eastern region, first-tier, and new first-tier cities, and varied across major migration zones. Further, the varying policy tools in the new talent settlement policies had disparate effects on housing prices. Thus, we recommend that new talent settlement policies must be coordinated with the goals of real estate regulation and reasonable regional standards, and that the policy tools should be tailored according to the actual conditions of cities.
The role of science in a crisis: Talks by political leaders during the COVID-19 pandemic
Authors: Loner Enzo , Fattorini Eliana , Bucchi Massimiano
Published: March 2023

During the COVID-19 pandemic, science has been prominently featured in institutional communication and political agendas as never before. Governments substantially relied on scientific experts to analyze pandemic trends, develop anti-COVID-19 vaccines and adopt containment strategies. In this paper, we analyze speeches by three political leaders–Boris Johnson (Prime Minister, UK), Sergio Mattarella (President of the Republic, Italy), and Ursula von der Leyen (President, European Commission)–between February 20, 2020, and February 20, 2022, to identify how science was addressed and framed. The results of the quantitative and qualitative exploration of the speeches highlight three main ways in which political leaders view science: a national pride narrative–i.e., science as an instrument and indicator of national pride and international standing of the country; an ethical narrative–i.e., science as an agent of social growth; an integration narrative–i.e., science as a driving force of both European integration and stronger collaboration between knowledge production and industry. The predominant narrative varies in relation to the political leaders’ different institutional contexts and roles.
Reward systems for cohort data sharing: An interview study with funding agencies
Authors: Devriendt Thijs , Shabani Mahsa , Borry Pascal
Published: March 2023

Data infrastructures are being constructed to facilitate cohort data sharing. These infrastructures are anticipated to increase the rate of data sharing. However, the lack of data sharing has also been framed as being the consequence of the lack of reputational or financial incentives for sharing. Some initiatives try to confer value onto data sharing by making researchers’ individual contributions to research visible (i.e., contributorship) or by quantifying the degree to which research data has been shared (e.g., data indicators). So far, the role of downstream evaluation and funding distribution systems for reputational incentives remains underexplored. This interview study documents the perspectives of members of funding agencies on, amongst other elements, incentives for data sharing. Funding agencies are adopting narrative CVs to encourage evaluation of diverse research outputs and display diversity in researchers’ profiles. This was argued to diminish the focus on quantitative indicators of scientific productivity. Indicators related to open science dimensions may be reintroduced if they are fully developed. Shifts towards contributorship models for research outputs are seen as complementary to narrative review.
Identification of two unannotated miRNAs in classic Hodgkin lymphoma cell lines
Authors: Ustaszewski Adam , Paczkowska Julia , Janiszewska Joanna , Bernhart Stephan H. , Bein Julia , Russiñol Núria , Hansmann Martin-Leo , Chapaprieta Vicente , Martín-Subero José I. , Siebert Reiner , Hartmann Sylvia , Giefing Maciej
Published: March 2023

MicroRNAs (miRNAs) are small non coding RNAs responsible for posttranscriptional regulation of gene expression. Even though almost 2000 precursors have been described so far, additional miRNAs are still being discovered in normal as well as malignant cells. Alike protein coding genes, miRNAs may acquire oncogenic properties in consequence of altered expression or presence of gain or loss of function mutations. In this study we mined datasets from miRNA expression profiling (miRNA-seq) of 7 classic Hodgkin Lymphoma (cHL) cell lines, 10 non-Hodgkin lymphoma (NHL) cell lines and 56 samples of germinal center derived B-cell lymphomas. Our aim was to discover potential novel cHL oncomiRs not reported in miRBase (release 22.1) and expressed in cHL cell lines but no other B-cell lymphomas. We identified six such miRNA candidates in cHL cell lines and verified the expression of two of them encoded at chr2:212678788–212678849 and chr5:168090507–168090561 (GRCh38). Interestingly, we showed that one of the validated miRNAs (located in an intron of the TENM2 gene) is expressed together with its host gene. TENM2 is characterized by hypomethylation and open chromatin around its TSS in cHL cell lines in contrast to NHL cell lines and germinal centre B-cells respectively. It indicates an epigenetic mechanism responsible for aberrant expression of both, the TENM2 gene and the novel miRNA in cHL cell lines. Despite the GO analysis performed with the input of the in silico predicted novel miRNA target genes did not reveal ontologies typically associated with cHL pathogenesis, it pointed to several interesting candidates involved in i.e. lymphopoiesis. These include the lymphoma related BCL11A gene, the IKZF2 gene involved in lymphocyte development or the transcription initiator GTF2H1.
Physiotherapists’ practice patterns for the diagnosis and management of patients with chronic contracted frozen shoulder in the United Arab Emirates
Authors: Alhammadi Mariam J. , Hegazy Fatma A.
Published: March 2023

Background

Adhesive capsulitis or contracted shoulder, known as frozen shoulder, is a persistent painful condition that may last for more than three months. It is a common disease-causing morbidity that causes pain and loss of shoulder range of motion. Physical therapy is advocated for the restoration of a pain-free state and normal use of the upper extremity, along with other interventions.

Purpose

This study aimed to explore the level of current clinical practice for managing chronic contracted frozen shoulder (CCFS) among physiotherapy professionals in the United Arab Emirates (UAE) compared to well–established evidence-based practices, and to identify the most common therapy practiced in UAE to manage CCFS.

Method

This study was based on a cross-sectional quantitative analysis using an adapted questionnaire. The main themes of questions were the presence of a special interest in CCFS, management options, symptoms, diagnosis, referral, and best physiotherapy intervention recommendations. The results were analyzed using simple descriptive analyses, such as frequency, mean, and percentage of total responses; additionally, thematic and content analyses were performed for open-ended questions.

Results

Overall, 101 physiotherapy professionals participated in the survey: 62% female and 38% male; 59% were bachelors- and 36% masters-degree holders, respectively. In the closed ended question regarding the interest in CCFS, male physiotherapists (PTs) were more interested than females (82% vs. 68%). For the most common indication of CCFS, 76% of the participants selected “Limitation of movement" as the main indication. However, only 42% confirmed the presence of clinical protocols in their employment setting. In their opinion, the most effective therapies were patient education, superficial heat or cold, manual joint mobilization, and sustained stretching exercises.

Conclusion

A well-established professional competence exists among physiotherapists in the UAE to manage and treat patients with CCFS. The findings showed sufficient standard, theoretical, and practical knowledge among the study groups.
Improved rock phosphate dissolution from organic acids is driven by nitrate assimilation of bacteria isolated from nitrate and CaCO3-rich soil
Authors: Garcia-Sanchez Mercedes , Bertrand Isabelle , Barakat Abdellatif , Zeroual Youssef , Oukarroum Abdallah , Plassard Claude
Published: March 2023

Until now, the solubilization capacities of insoluble mineral P by soil microorganisms have been screened in vitro with media containing NH4+ as a nitrogen source. This presence of NH4+ will lead to an acidification of the medium responsible for the solubilization of the insoluble P. However, besides proton release, the production of organic acids can play a very important role in the release of free P. This physiological mechanism can largely depend on the source of nitrogen (NH4+vs NO3-) assimilated by the bacteria but the influence of the N source on the production of organic acids has yet to be studied. Our aim was to investigate if the N source assimilated by bacteria and the soil characteristics such as the dominant N source (NH4+vs NO3-) and CaCO3 contents might influence the bacterial capacities to solubilize rock phosphate. To fill this objective, we screened the capacity of bacteria isolated from 3 soils to solubilize rock phosphate in vitro in presence of NH4+or NO3-. Then, we selected the most efficient bacterial strains to identify and quantify the release of organic anions into the medium. Among the two hundred and forty-three bacterial strains isolated from the 3 soils, nine and seven isolates were identified with the highest % rock phosphate-solubilization values with NH4+ or NO3- as the sole N-source. Only one strain was able to release free Pi with NH4+ or NO3- as the sole N-source. The most predominant organic acids released by almost all isolates were gluconic acid, lactic acid, glycolic acid, acetic acid, formic acid and pyruvic acid regardless the N-source. However, with NO3- as source of N, the highest concentrations on those acids were found together with the highest release of free Pi into the medium. Molecular analysis of 16S rRNA indicated that almost all strains belonged to Bacillus and Paenibacillus genera. The PCA analysis between soil properties and bacterial capacities to release organic acids and free Pi also revealed that soil factors such as CaCO3 and soil NO3- content positively influenced the release of organic acids by bacteria grown in vitro. Our results concluded that the bacterial rock phosphate-solubilization was intimately related to organic acids production which in turn seemed to be driven by the assimilation of NO3- by bacteria. Therefore, the N-source might be considered a key factor to take into consideration during the screening and selection of suitable strains involved in the P-solubilization.
Factors correlated with pain after total knee arthroplasty: A systematic review and meta-analysis
Authors: Olsen Unni , Lindberg Maren Falch , Rose Christopher , Denison Eva , Gay Caryl , Aamodt Arild , Brox Jens Ivar , Skare Øystein , Furnes Ove , Lee Kathryn A. , Lerdal Anners
Published: March 2023

Main objective

Systematically review and synthesize preoperative and intraoperative factors associated with pain after total knee arthroplasty (TKA) in patients with osteoarthritis.

Methods

Based on a peer-reviewed protocol, we searched Medline, Embase, CINAHL, Cochrane Library, and PEDro for prospective observational studies (January 2000 to February 2023) investigating factors associated with pain after TKA. The primary outcome was pain twelve months after TKA. Pain at three and six months were secondary outcomes. Multivariate random-effects meta-analyses were used to estimate mean correlation (95% CIs) between factors and pain. Sensitivity analysis was performed for each risk of bias domain and certainty of evidence was assessed.

Results

Of 13,640 studies, 29 reports of 10,360 patients and 61 factors were analysed. The mean correlation between preoperative factors and more severe pain at twelve months was estimated to be 0.36 (95% CI, 0.24, 0.47; P < .000; moderate-certainty evidence) for more catastrophizing, 0.15 (95% CI; 0.08, 0.23; P < .001; moderate-certainty evidence) for more symptomatic joints, 0.13 (95% CI, 0.06, 0.19; P < .001; very low-certainty evidence) for more preoperative pain. Mean correlation between more severe radiographic osteoarthritis and less pain was -0.15 (95% CI; -0.23, -0.08; P < .001; low-certainty evidence). In sensitivity analysis, the estimated correlation coefficient for pain catastrophizing factor increased to 0.38 (95% CI 0.04, 0.64). At six and three months, more severe preoperative pain was associated with more pain. Better preoperative mental health was associated with less pain at six months.

Conclusion and relevance

More pain catastrophizing, more symptomatic joints and more pain preoperatively were correlated with more pain, while more severe osteoarthritis was correlated with less pain one year after TKA. More preoperative pain was correlated with more pain, and better mental health with less pain at six and three months. These findings should be further tested in predictive models to gain knowledge which may improve TKA outcomes.
The spatial and temporal variation of fine particulate matter pollution in Ethiopia: Data from the Atmospheric Composition Analysis Group (1998–2019)
Authors: Shiferaw Ashenafie Bereded , Kumie Abera , Tefera Worku
Published: March 2023

Background

Evidence suggests ambient fine particulate matter (PM2.5) is a risk factor for cardiovascular diseases, lung cancer morbidity and mortality, and all-cause mortality. Countries that implement strong policies are able to reduce ambient PM2.5 concentration. In Ethiopia, however, PM2.5 monitoring stations, laboratory technicians, and equipment are staggeringly limited. In this study, the spatial and temporal variation of PM2.5 in Ethiopia was assessed.

Methods

Satellite-based PM2.5 estimates, from the year 1998 to 2019, by Atmospheric Composition Analysis Group (ACAG) at a spatial resolution of 0.01° X 0.01° was used. The annual mean PM2.5 concentration for all administrative regions and zones in Ethiopia was extracted. The average mean from the twenty-two years was also calculated. The trend of PM2.5 concentration was graphed and quantitatively calculated using the Mann-Kendall test. The slope of the change over time was estimated using the Theil-Sen slope. At the zonal administration level, for the average annual mean, spatial dependency using univariate Global Moran’s I and clustering and outlier tests using Anselin Local Moran’s were performed.

Results

The country’s average annual mean PM2.5 concentration was 17 μgm-3. The Afar region had the highest concentration, 27.9 μgm-3. The Mann-Kendall S was positive and significant at p<0.001. The spatial distribution of satellite-based ambient PM2.5 concentration was non-random. Significant highest value clustering of ambient total PM2.5 concentration exists in the Afar, Eastern Tigray, and Eastern and Southeastern Amhara while the significant lowest value dispersing was observed in the Southern Oromia and Somali region.

Conclusion

At the national and regional levels, the annual mean ambient PM2.5 concentration is beyond the World Health Organization (WHO)-recommended level. The ambient PM2.5 concentration distribution is spatially dependent and significantly clustered in space. Installation of additional ground-based PM2.5 monitoring devices, particularly in regions where PM2.5 concentration is higher, is recommended. Validating satellite-based PM2.5 data with ground-based measurements in the country is also advised.
PSMD11 modulates circadian clock function through PER and CRY nuclear translocation
Authors: Cal-Kayitmazbatir Sibel , Francey Lauren J. , Lee Yool , Liu Andrew C. , Hogenesch John B.
Published: March 2023

The molecular circadian clock is regulated by a transcriptional translational feedback loop. However, the post-translational control mechanisms are less understood. The NRON complex is a large ribonucleoprotein complex, consisting of a lncRNA and several proteins. Components of the complex play a distinct role in regulating protein phosphorylation, synthesis, stability, and translocation in cellular processes. This includes the NFAT and the circadian clock pathway. PSMD11 is a component of the NRON complex and a lid component of the 26S proteasome. Among the PSMD family members, PSMD11 has a more specific role in circadian clock function. Here, we used cell and biochemical approaches and characterized the role of PSMD11 in regulating the stability and nuclear translocation of circadian clock proteins. We used size exclusion chromatography to enrich the NRON complex in the cytosolic and nuclear fractions. More specifically, PSMD11 knockdown affected the abundance of PER2 and CRY2 proteins and the nuclear translocation of CRY1. This changed the relative abundance of CRY1 and CRY2 in the nucleus. Thus, this work defines the role of PSMD11 in the NRON complex regulating the nuclear translocation of circadian repressors, thereby enabling cellular circadian oscillations.
Prevalence of undernutrition and associated factors among adults taking antiretroviral therapy in sub-Saharan Africa: A systematic review and meta-analysis
Authors: Seid Awole , Seid Omer , Workineh Yinager , Dessie Getenet , Bitew Zebenay Workneh
Published: March 2023

Background

Undernutrition (Body Mass Index < 18.5 kg/m2) is a common problem and a major cause of hospital admission for patients living with HIV. Though sub-Saharan Africa is the most commonly affected region with HIV and malnutrition, a meta-analysis study that estimates the prevalence and correlates of undernutrition among adults living with HIV has not yet been conducted. The objective of this study was to determine the pooled prevalence of undernutrition and associated factors among adults living with HIV/AIDS in sub-Saharan Africa.

Methods

Studies published in English were searched systematically from databases such as PubMed, Google Scholar, and gray literature, as well as manually from references in published articles. Observational studies published from 2009 to November 2021 were included. The data extraction checklist was prepared using Microsoft Excel and includes author names, study area, publication year, sample size, prevalence/odds ratio, and confidence intervals. The results were presented and summarized in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) standard. Heterogeneity was investigated using the Q test, I2, τ2, τ and predictive interval. STATA version 17 was used to analyze the data. A meta-analysis using a random-effects model was used to determine the overall prevalence and adjusted odds ratio. The study has been registered in PROSPERO with a protocol number of CRD42021268603.

Results

In this study, a total of 44 studies and 22,316 participants were included. The pooled prevalence of undernutrition among adult people living with HIV (PLWHIV) was 23.72% (95% CI: 20.69–26.85). The factors associated with undernutrition were participants’ age (AOR = 0.5, 95% CI: 0.29–0.88), gender (AOR = 2.08, 95% CI: 0.22–20.00), World Health Organization (WHO) clinical stage (AOR = 3.25, 95% CI: 2.57–3.93), Cluster of Differentiation 4 (CD4 count) (AOR = 1.94, 95% CI: 1.53–2.28), and duration of ART (AOR = 2.32, 95% CI: 1.6–3.02).

Conclusion

The pooled prevalence of undernutrition among adult PLWHIV in sub-Saharan Africa remained high. WHO clinical stage, CD4 count, duration of ART treatment, age, and sex were found to be the factors associated with undernutrition. Reinforcing nutrition counseling, care, and support for adults living with HIV is recommended. Priority nutritional screening and interventions should be provided for patients with advanced WHO clinical stages, low CD4 counts, the male gender, younger age groups, and ART beginners.
Recommendations for an exercise intervention and core outcome set for older patients after hospital discharge: Results of an international Delphi study
Authors: Aarden Jesse J. , Major Mel E. , Aghina Claartje M. W. , Esch Martin van der , Buurman Bianca M. , Engelbert Raoul H. H. , Schaaf Marike van der
Published: March 2023

For older adults, acute hospitalization is a high-risk event with poor health outcomes, including functional decline. In absence of practical guidelines and high quality randomized controlled trials, this Delphi study was conducted. The aim of this study was to obtain consensus on an exercise intervention program, a core outcome set (COS) and handover information to prevent functional decline or restore physical function in acutely hospitalized older patients transitioning from hospital to home. An internal panel of experts in the field of exercise interventions for acutely hospitalized older adults were invited to join the study. In the Delphi study, relevant topics were recognized, statements were formulated and ranked on a 9-point Likert scale in two additional rounds. To reaching consensus, a score of 7–9 was classified as essential. Results were expressed as median and semi-interquartile range (SIQR), and consensus threshold was set at SIQR≤0.5. Fifteen international experts from eight countries participated in the panel. The response rate was 93%, 93% and 80% for the three rounds respectively. After three rounds, consensus was reached on 167 of the 185 (90.3%) statements, of which ninety-five (51.4%) were ranked as essential (median Likert-score ≥7.0, SIQR ≤0.5). This Delphi study provides starting points for developing an exercise intervention, a COS and handover information. The results of this Delphi study can assist physical therapists to provide a tailored exercise intervention for older patients with complex care needs after hospital discharge, to prevent functional decline and/or restore physical function.
HIV retesting for pregnant and breastfeeding women across maternal child health services in Nampula, Mozambique
Authors: Teasdale Chloe A. , Choy Michelle , Tsiouris Fatima , De Gusmao Eduarda Pimentel , Banqueiro Etelvino C. P. , Couto Aleny , Tibana Kwalila , Flowers Nicole , Urso Marilena , Vitale Mirriah , Abrams Elaine J.
Published: March 2023

Background

Repeat HIV testing during pregnancy and breastfeeding identifies women with incident infections, those living with HIV who have been lost to care, and infants at risk for HIV infection. We report data from repeat testing for women in maternal and child health (MCH) services at 10 health facilities in Mozambique.

Methods

Routinely collected data from health facility registers are reported from April-November 2019. From antenatal care (ANC), we report numbers and proportions of women eligible for retesting; returned for care when retesting eligible; retested; and HIV-positive (HIV+) at retesting. From child welfare clinics (CWC), we report mothers retested; tested HIV-positive; HIV+ mothers linked to ART services; HIV-exposed infants (HEI) tested for HIV with polymerase chain reaction (PCR) tests; HEI testing PCR positive; PCR-positive infants linked to care.

Results

In ANC, 28,233 pregnant women tested HIV-negative at first ANC visit, 40.7% had a follow-up visit when retesting eligible, among whom 84.8% were retested and 0.3%(N = 26) tested HIV+. In CWC, 26,503 women were tested; 0.8%(N = 212) tested HIV+ and 74.1%(N = 157) of HIV+ women were linked to care. Among 157 HEI identified in CWC, 68.4%(N = 145) received PCR testing and 19.3%(N = 28) tested positive.

Conclusion

In ANC, less than half of pregnant women eligible for retesting returned for follow-up visits, and test positivity was low among women retested in ANC and CWC. In CWC, linkage to infant testing was poor and almost 20% of HEI were PCR-positive. Implementing retesting for pregnant and breastfeeding women is challenging due to high numbers of women and low testing yield.
Field assessment of BinaxNOW antigen tests as COVID-19 treatment entry point at a community testing site in San Francisco during evolving omicron surges
Authors: Schrom John , Marquez Carina , Wang Chung-Yu , Saxena Aditi , Mitchell Anthea M. , Ribeiro Salu , Pilarowski Genay , Nakamura Robert , Rojas Susana , Black Douglas , Contreras Oseguera Maria G. , Diaz Edgar Castellanos , Payan Joselin , Rojas Susy , Jones Diane , Tulier-Laiwa Valerie , Zavaleta Aleks , Martinez Jacqueline , Chamie Gabriel , Glaser Carol , Jacobson Kathy , Petersen Maya , DeRisi Joseph , Havlir Diane V.
Published: March 2023

COVID-19 oral treatments require initiation within 5 days of symptom onset. Although antigen tests are less sensitive than RT-PCR, rapid results could facilitate entry to treatment. We collected anterior nasal swabs for BinaxNOW and RT-PCR testing and clinical data at a walk-up, community site in San Francisco, California between January and June 2022. SARS-CoV-2 genomic sequences were generated from positive samples and classified according to subtype and variant. Monte Carlo simulations were conducted to estimate the expected proportion of SARS-CoV-2 infected persons who would have been diagnosed within 5 days of symptom onset using RT-PCR versus BinaxNOW testing. Among 25,309 persons tested with BinaxNOW, 2,799 had concomitant RT-PCR. 1137/2799 (40.6%) were SARS-CoV-2 RT-PCR positive. We identified waves of predominant omicron BA.1, BA.2, BA.2.12, BA.4, and BA.5 among 720 sequenced samples. Among 1,137 RT-PCR positive samples, 788/1137 (69%) were detected by BinaxNOW; 94% (669/711) of those with Ct value <30 were detected by BinaxNOW. BinaxNOW detection was consistent over lineages. In analyses to evaluate entry to treatment, BinaxNOW detected 81.7% (361/442, 95% CI: 77–85%) of persons with COVID-19 within 5 days of symptom onset. In comparison, RT-PCR (24-hour turnaround) detected 84.2% (372/442, 95% CI: 80–87%) and RT-PCR (48-hour turnaround) detected 67.0% (296/442, 95% CI: 62–71%) of persons with COVID-19 within 5 days of symptom onset. BinaxNOW detected high viral load from anterior nasal swabs consistently across omicron sublineages emerging between January and June of 2022. Simulations support BinaxNOW as an entry point for COVID-19 treatment in a community field setting.
Potent NKT cell ligands overcome SARS-CoV-2 immune evasion to mitigate viral pathogenesis in mouse models
Authors: Lu Hongjia , Liu Zhewei , Deng Xiangxue , Chen Siyang , Zhou Ruiting , Zhao Rongqi , Parandaman Ramya , Thind Amarjot , Henley Jill , Tian Lei , Yu Jianhua , Comai Lucio , Feng Pinghui , Yuan Weiming
Published: March 2023

One of the major pathogenesis mechanisms of SARS-CoV-2 is its potent suppression of innate immunity, including blocking the production of type I interferons. However, it is unknown whether and how the virus interacts with different innate-like T cells, including NKT, MAIT and γδ T cells. Here we reported that upon SARS-CoV-2 infection, invariant NKT (iNKT) cells rapidly trafficked to infected lung tissues from the periphery. We discovered that the envelope (E) protein of SARS-CoV-2 efficiently down-regulated the cell surface expression of the antigen-presenting molecule, CD1d, to suppress the function of iNKT cells. E protein is a small membrane protein and a viroporin that plays important roles in virion packaging and envelopment during viral morphogenesis. We showed that the transmembrane domain of E protein was responsible for suppressing CD1d expression by specifically reducing the level of mature, post-ER forms of CD1d, suggesting that it suppressed the trafficking of CD1d proteins and led to their degradation. Point mutations demonstrated that the putative ion channel function was required for suppression of CD1d expression and inhibition of the ion channel function using small chemicals rescued the CD1d expression. Importantly, we discovered that among seven human coronaviruses, only E proteins from highly pathogenic coronaviruses including SARS-CoV-2, SARS-CoV and MERS suppressed CD1d expression, whereas the E proteins of human common cold coronaviruses, HCoV-OC43, HCoV-229E, HCoV-NL63 and HCoV-HKU1, did not. These results suggested that E protein-mediated evasion of NKT cell function was likely an important pathogenesis factor, enhancing the virulence of these highly pathogenic coronaviruses. Remarkably, activation of iNKT cells with their glycolipid ligands, both prophylactically and therapeutically, overcame the putative viral immune evasion, significantly mitigated viral pathogenesis and improved host survival in mice. Our results suggested a novel NKT cell-based anti-SARS-CoV-2 therapeutic approach.