bluuebunny/crossref_metadata_2025 huggingface dataset:
Valorising lignocellulosic biomass to high-performance electrocatalysts via anaerobic digestion pretreatment
Authors: Yang Juntao , Tang Songbiao , Mei Wenjie , Chen Yiquan , Yi Weiming , Lv Pengmei , Yang Gaixiu
Published: March 2024

Anaerobic digestion (AD) was initially evaluated as a potential preprocessing method for preparing biomass-based carbon electrocatalysts in this study. The AD pretreatment succeeded in the structural depolymerization and nitrogen enrichment of Hybrid Pennisetum, which provided favorable conditions to achieve efficient and homogeneous nitrogen introduction due to microorganism community enrichment and provided a porous structure by degradation of the biodegradable components. The resulted biochar exhibited improved physiochemical properties including higher specific surface areas, nitrogen content and graphitization degree than that obtained from pyrolyzing raw biomass. These improvements were positively correlated with the AD time and showed to have enhanced the performance in oxygen reduction reaction and practical microbial fuel cell  applications. Amongst the investigated samples, the obtained biochar pretreated by AD for 15 days exhibited the most excellent performance with an onset potential of 0.17 V (VS. saturated calomel electrode) and the maximal power density of 543.2 mW cm−2 assembled in microbial fuel cells. This study suggested applying AD as a new biological pretreatment in the preparation of biomass-based electrocatalysts, and provided a unique pathway for fabricating high-performance biochar-based catalysts by structure optimization and N-containing active sites construction via gentle biological method, thereby providing a cost-effective method to fabricate metal-free catalysts for oxygen reduction reaction.

Graphical Abstract
Selection of Academic Staff Based on a Hybrid Multi-criteria Decision Method Under Neutrosophic Environment
Authors: Paraskevas Antonios , Madas Michael
Published: March 2024

Multi-criteria decision-making (MCDM) is a decision-making process that involves assessing and selecting the best alternative from a group of options based on various criteria or qualities. In this research work, we propose and elucidate the theory of neutrosophic logic, which is unique in its approach to evaluating candidates’ performance in a manner that takes into account significant elements and criteria that are essential for the overall process when dealing with unclear, inaccurate, or incomplete data. We propose a novel hybrid integrated MCDM methodology based upon neutrosophic Delphi (N-Delphi) and neutrosophic AHP (N-AHP) methods, which takes into consideration the importance of each decision-maker and their preferences per evaluation criterion. A new MAXMIN threshold value technique treats the criteria under consideration as the decision alternatives and their score functions as their payoff values, thus reducing unnecessary resources by eliminating unimportant criteria during the personnel selection process.
The network resilience of safety confirmation and social capital during natural disasters: the comparison of the U.S. and Japan
Authors: Kawamoto Kiyomi , Morisugi Masafumi , Yamashita Eric Y.
Published: March 2024

This study discusses safety confirmation systems and how Social Capital (SC) affects the resilience of the safety confirmation network for natural disasters in the U.S. and Japanese society. When a large-scale disaster occurs, people desire to inform their loved ones of their safety status and search for their loved one’s status in a chaotic environment. Safety confirmation is the action of confirming the current status of people in disaster areas by collecting safety, injury, missing, and death information and sending the contact request. First, this study summarized the safety confirmation resources. The U.S. approach focuses on the missing and dead, while the Japanese approach focuses on the survivors and tries to define the remaining people who are missing and dead. Second, the social background that formed the SC was compared. Third, how SC affects the safety confirmation network in and between communities was analyzed. The case areas were Honolulu County in the U.S. and Aichi prefecture in Japan, and 1,324 samples were analyzed by ordinal logistic regression. The safety confirmation network reflected the social and SC structure for each country. The ethnic diversity in the network provided high resilience. Honolulu’s network was more open beyond the community and connected more with others. Residents’ economic network was linked to the safety confirmation network. Aichi’s network was more closed in the community and connected with kinship or neighbor ties. Trust and interaction with others shaped the foundation of the safety confirmation network. Finally, the integrated safety confirmation system was discussed.
American idle: the relative importance of dispositional and environmental predictors of state labor force participation rates in the USA
Authors: McCann Stewart J. H.
Published: March 2024

This is the first study to determine the capacity of state-level IQ and Big Five personality to predict total, male, and female state labor force participation rates (LFPRs). It is based on residents aged 20–64 years in the 48 contiguous American states from 2013 to 2017. Five state environmental variables—White population percent, urban population percent, per capita personal income, political preference, and age distribution—were statistically controlled. Multiple regression analysis revealed that IQ independently predicted total, male, and female LFPRs with β coefficients of 0.67, 0.71, and 0.59, respectively. Neuroticism also independently predicted total LFPRs and male LFPRs with βs of − 0.20 and − 0.29. As well, lower Openness to Experience and higher liberal political preference were associated with higher female LFPRs, producing βs of − 0.37 and 0.47, respectively. No other variables emerged as independent predictors. Regarding total LFPR variance, the six dispositional variables jointly accounted for 23.0% with the five environmental variables controlled and 74.2% without. Corresponding values were 25.9% and 74.3% for male LFPRs, and 19.6% and 66.5% for female LFPRs. With the six dispositional variables controlled, the five environmental variables together could only account for 11.0% in total LFPRs, 10.8% in male LFPRs, and 16.5% in female LFPRs. Spatial autocorrelation was tested and found to be nonsignificant. These previously overlooked dispositional predictors of state LFPRs are especially important given the salience of LFPRs in economic functioning, declines in American LFPRs since 2000, and the 35th-place standing of the USA on LFPRs among nations by 2022.
Mass Gathering Event Medical Preparedness and Response: A Review of Canadian Legislation and Guidelines
Authors: Pigeon Marc-Antoine , Hertelendy Attila , Hart Alexander , Lund Adam , Ranse Jamie , Ciottone Gregory
Published: March 2024

Introduction:

The mass gathering event (MGE) industry is growing globally, including in countries such as Canada. MGEs have been associated with a greater prevalence of injury and illness when compared with daily life events, despite most participants having few comorbidities. As such, adequate health, safety, and emergency medical planning is required. However, there is no single entity regulating these concerns for MGEs, resulting in the responsibility for health planning lying with event organizers. This study aims to compare the legislative requirements for MGE medical response systems in the 13 provinces and territories of Canada.

Methods:

This study is a cross-sectional descriptive analysis of Canadian legislation. Lists of publicly available legislative requirements were obtained by means of the emergency medical services directors and Health Ministries. Descriptive statistics were performed to compare legislation.

Results:

Of the 13 provinces and territories, 10 responded. For the missing 3, a law library review confirmed the absence of specific legislation. Most (n = 6; 60%) provinces and territories referred to provisions in their Public Health laws. Four confirmed that MGE medical response was a municipal or local concern to be addressed by the event organizers.

Conclusions:

No provinces could list specific legislation guiding safety, health, and medical response for an MGE.
Stakeholder perspectives of mental healthcare services in Bangladesh, its challenges and opportunities: a qualitative study
Authors: Koly Kamrun Nahar , Saba Jobaida , Rao Mala , Rasheed Sabrina , Reidpath Daniel D. , Armstrong Stephanie , Gnani Shamini
Published: March 2024

This study explores Bangladesh’s mental health services from an individual- and system-level perspective and provides insights and recommendations for strengthening it’s mental health system. We conducted 13 in-depth interviews and 2 focus group discussions. Thirty-one participants were recruited using a combination of purposive and snowball sampling methods. All interviews and group discussions were audio-recorded and transcribed, and key findings were translated from Bengali to English. Data were coded manually and analysed using a thematic and narrative analysis approach. Stakeholders perceived scarcity of service availability at the peripheral level, shortage of professionals, weak referral systems, lack of policy implementation and regulatory mechanisms were significant challenges to the mental health system in Bangladesh. At the population level, low levels of mental health literacy, high societal stigma, and treatment costs were barriers to accessing mental healthcare. Key recommendations included increasing the number of mental health workers and capacity building, strengthening regulatory mechanisms to enhance the quality of care within the health systems, and raising awareness about mental health. Introducing measures that relate to tackling stigma, mental health literacy as well as building the capacity of the health workforce and governance systems will help ensure universal mental health coverage.
Stable production of fluid jets with vanishing diameters via tip streaming
Authors: Rubio M. , Montanero J.M. , Eggers J. , Herrada M.A.
Published: March 2024

We study numerically the microjetting mode obtained when a fluid is injected through a tube submerged in a uniaxial extensional flow. The steady solution to the full nonlinear Navier–Stokes equations is calculated. We obtain the linear global modes determining the linear stability of the steady solution. For sufficiently large outer viscosity, the flow remains stable for infinitely small values of the injected flow rate. This implies that jets with vanishing diameters can be produced regardless of the jet viscosity and outer flow strength. For a sufficiently small inner-to-outer viscosity ratio, the microjetting instability is associated only with the flow near the entrance of the jet. The tapering meniscus stretches and adopts a slender quasiconical shape. Consequently, the cone tip is exposed to an intense outer flow, which stabilizes the flow in the cone–jet transition region. This work presents the first evidence that fluid jets with arbitrarily small diameters can be stably produced via tip streaming. The results are related to those of a droplet in a uniaxial extensional flow with its equator pinned to an infinitely thin ring. The pinning of the equator drastically affects the droplet stability and breakup.
Activism across Borders: A Human Rights Perspective
Authors: Hurst Mark
Published: March 2024

Daniel Laqua's recent monograph Activism across Borders Since 1870: Causes, Campaigns and Conflicts in and beyond Europe raises a number of pertinent issues for historians of human rights to reflect upon. This article takes the four analytical lenses highlighted by Laqua for assessing transnational activism and applies them to cases of human rights activism in the Cold War and post-Cold War era. In doing so, this article argues that Laqua's framework offers much scope for historians to approach the history of human rights activism with a more critical edge. It also highlights the challenge of retaining an analytical focus on an issue as emotive and complex as human rights, and how Laqua's lenses may offer a practical methodology to do this.
Genetic characterization of the Entamoeba moshkovskii population based on different potential genetic markers
Authors: Sardar Sanjib K. , Ghosal Ajanta , Haldar Tapas , Prasad Akash , Mal Sweety , Saito-Nakano Yumiko , Kobayashi Seiki , Dutta Shanta , Nozaki Tomoyoshi , Ganguly Sandipan
Published: March 2024

Entamoeba moshkovskii, according to recent studies, appears to exert a more significant impact on diarrhoeal infections than previously believed. The efficient identification and genetic characterization of E. moshkovskii isolates from endemic areas worldwide are crucial for understanding the impact of parasite genomes on amoebic infections. In this study, we employed a multilocus sequence typing system to characterize E. moshkovskii isolates, with the aim of assessing the role of genetic variation in the pathogenic potential of E. moshkovskii. We incorporated 3 potential genetic markers: KERP1, a protein rich in lysine and glutamic acid; amoebapore C (apc) and chitinase. Sequencing was attempted for all target loci in 68 positive E. moshkovskii samples, and successfully sequenced a total of 33 samples for all 3 loci. The analysis revealed 17 distinct genotypes, labelled M1–M17, across the tested samples when combining all loci. Notably, genotype M1 demonstrated a statistically significant association with diarrhoeal incidence within E. moshkovskii infection (P = 0.0394). This suggests that M1 may represent a pathogenic strain with the highest potential for causing diarrhoeal symptoms. Additionally, we have identified a few single-nucleotide polymorphisms in the studied loci that can be utilized as genetic markers for recognizing the most potentially pathogenic E. moshkovskii isolates. In our genetic diversity study, the apc locus demonstrated the highest Hd value and π value, indicating its pivotal role in reflecting the evolutionary history and adaptation of the E. moshkovskii population. Furthermore, analyses of linkage disequilibrium and recombination within the E. moshkovskii population suggested that the apc locus could play a crucial role in determining the virulence of E. moshkovskii.
Associations between body weight change and incidence of major depressive disorder in patients with type 2 diabetes mellitus: a nationwide longitudinal follow-up cohort study of 1.1 million
Authors: Kim Eun Young , Lee You-Bin , Lee Gyu-Na , Han Kyungdo , Jeong Su-Min
Published: March 2024

Background

Comorbid depression substantially affects the management of glycemia and diabetes-related complications among patients with type 2 diabetes mellitus. In this study, we sought to determine the association between weight change over 4 years and depression risk among patients with type 2 diabetes mellitus.

Methods

This population-based retrospective cohort study from the National Health Insurance Services of Korea included 1 111 345 patients with type 2 diabetes who were divided into groups according to body weight change over 4 years. Body weight changes were compared with the preceding 4-year period (2005–2008). Depression was defined according to the International Classification of Diseases 10th revision code for depression (F32 and F33) on one or more inpatient or outpatient claims.

Results

During a median follow-up of 7.4 years, 244 081 cases of depression were identified. We observed a U-shaped association between body weight change and depression risk with a higher risk among both groups of weight loss (hazard ratio (HR) 1.17, 95% CI 1.15–1.19 for ⩾ −10%; HR 1.07, 95% CI 1.06–1.08 for −10 to −5%) and weight gain (HR 1.06, 95% CI 1.04–1.08 for ⩾10%; HR 1.02, 95% CI 1.01–1.04 for 5–10%) compared with the stable weight group (−5 to 5%).

Conclusions

A U-shaped association between body weight change and depression risk was observed in this large nationwide cohort study. Our study suggests that patients with type 2 diabetes and weight change, either gain or loss, could be considered a high-risk group for depression.
Retinal microvascular function and incidence and trajectories of clinically relevant depressive symptoms: the Maastricht Study
Authors: van Gennip April C. E. , Gupta Monideepa D. , Houben Alfons J. H. M. , Berendschot Tos T. J. M. , Webers Carroll A. B. , van Greevenbroek Marleen M. J. , van der Kallen Carla J. H. , Koster Annemarie , Wesselius Anke , Eussen Simone J. P. M. , Schalkwijk Casper G. , de Galan Bastiaan E. , Köhler Sebastian , Schram Miranda T. , Stehouwer Coen D. A. , van Sloten Thomas T.
Published: March 2024

Background

Cerebral microvascular dysfunction may contribute to depression via disruption of brain structures involved in mood regulation, but evidence is limited. We investigated the association of retinal microvascular function, a proxy for microvascular function in the brain, with incidence and trajectories of clinically relevant depressive symptoms.

Methods

Longitudinal data are from The Maastricht Study of 5952 participants (59.9 ± 8.5 years/49.7% women) without clinically relevant depressive symptoms at baseline (2010–2017). Central retinal arteriolar equivalent and central retinal venular equivalent (CRAE and CRVE) and a composite score of flicker light-induced retinal arteriolar and venular dilation were assessed at baseline. We assessed incidence and trajectories of clinically relevant depressive symptoms (9-item Patient Health Questionnaire score ⩾10). Trajectories included continuously low prevalence (low, n = 5225 [87.8%]); early increasing, then chronic high prevalence (early-chronic, n = 157 [2.6%]); low, then increasing prevalence (late-increasing, n = 247 [4.2%]); and remitting prevalence (remitting, n = 323 [5.4%]).

Results

After a median follow-up of 7.0 years (range 1.0–11.0), 806 (13.5%) individuals had incident clinically relevant depressive symptoms. After full adjustment, a larger CRAE and CRVE were each associated with a lower risk of clinically relevant depressive symptoms (hazard ratios [HRs] per standard deviation [s.d.]: 0.89 [95% confidence interval (CI) 0.83–0.96] and 0.93 [0.86–0.99], respectively), while a lower flicker light-induced retinal dilation was associated with a higher risk of clinically relevant depressive symptoms (HR per s.d.: 1.10 [1.01–1.20]). Compared to the low trajectory, a larger CRAE was associated with lower odds of belonging to the early-chronic trajectory (OR: 0.83 [0.69–0.99]) and a lower flicker light-induced retinal dilation was associated with higher odds of belonging to the remitting trajectory (OR: 1.23 [1.07–1.43]).

Conclusions

These findings support the hypothesis that cerebral microvascular dysfunction contributes to the development of depressive symptoms.
Understanding sustained adoption of conservation agriculture among smallholder farmers: insights from a sentinel site in Malawi
Authors: Pangapanga-Phiri Innocent , Ngoma Hambulo , Thierfelder Christian
Published: March 2024

Smallholder farming systems need climate-proofing and sustainable intensification practices such as conservation agriculture (CA), are promising options. However, there is a general perception that the adoption of CA systems in southern Africa is low. Sentinel sites, where CA has been promoted for a long time, offer forward-looking new insights. This paper, thus, takes a deep dive at Nkhotakota district of Malawi to understand what could have led to the success of CA promotion and subsequent perceived high adoption. We use survey data from 620 farmers, with 298 farmers sampled from treatment areas – known to have had contact with host farmers and 320 from a control group. Overall, 31% of the farmers in both groups adopted full CA over at least a 2-year period. We also find that about 57% of farmers in the treatment area adopted full CA and only 7% of farmers in the control areas. This highlights that longer-term CA promotion with dedicated extension support can enhance the uptake of CA practices. In essence, this paper offers a different perspective to the current narrative that CA systems are too complex and knowledge intensive to be adopted despite its long-term promotion and significant investments. However, there are some nuances: sustained adoption even in sentinel sites is neither 100% nor persistent over the long term. We find an appreciable adoption decay, showing large declines from highs of 57 and 7% in adoption for at least 2 years for treatment and control, respectively, to 12% in the treatment group and practically zero in the control when we condition full CA adoption to at least 7 years. This means that fewer farmers adopted CA for a longer period and suggests some dis-adoption over time even in sentinel sites. The key adoption enablers in the sentinel sites include the availability of training, dedicated longer-term extension support coupled with farmer experiential learning through demonstration plots managed by host farmers. Based on our findings, there is need to consistently promote CA using farmer-centric approaches that include peer-to-peer learning over long periods. This allows farmers time to experiment with different CA options, enable behavioral and lasting change. At policy level, there is need to build and strengthen farmer groups to facilitate easier access to inputs like leguminous crop seeds for farmers practicing CA and to offer market-smart incentives to induce initial adoption in the short term to facilitate sustained adoption.
Power and alterity: Depictions of the Vascones from antiquity to the middle ages
Authors: Aguirresarobe Asier H.
Published: March 2024

This article undertakes an examination of the origins and evolution of a discourse of alterity against the Vascones –the alleged forefathers of the Basques – and other Western Pyrenean peoples from Antiquity to the Middle Ages. The methodology employed involves the study of literary references made to these peoples, which are then compared to recent scholarly and archeological evidence. Through this analysis, it becomes possible to evaluate the accuracy of these mentions and interpret them within their specific historical context. The results of this research indicate that mentions of the Vascones during this timeframe were mainly polemic in nature and lacked substantial grounding in empirical reality. Instead, it seems that the underlying objective of these narratives of alterity was twofold: to enhance the social and political standing of their authors and to support their claims to political control over the Western Pyrenees. The abandonment of these interpretative repertoires during the tenth century, coinciding with the emergence of the kingdom of Pamplona and the county-duchy of Wasconia, further emphasizes the connection between the display of these tropes and imbalances in political power between the region and its neighbors. The conclusions of this article directly challenge the underpinnings of discourses that depict the ancient Vascones as entirely alien to the political and religious paradigms derived from the Roman and Christian traditions. In so doing, it thus confront narratives about these “ancient Basques” that are prevalent in contemporary Basque cultural production.
Atypical Seasonality of the Silicon Cycle in the Yellow River Estuary and Bohai Sea Revealed by Stable Silicon Isotopes
Authors: Cui Quanchao , Liu Xiaowen , Wang Zhenyan , Sun Weidong , Xiao Yuanyuan , Sun Xiaole
Published: March 2024

Biogeochemical Si cycle in coastal areas is of vital importance due to its close link with the carbon cycle. However, the coastal Si cycle has been heavily perturbated by human activities. In this study, we studied the spatiotemporal distribution of biogenic Si (BSi) and dissolved Si (DSi) combined with stable Si isotopes of DSi (δ30SiDSi) in the Yellow River estuary and Bohai Sea, one of the most populated coastal areas in the world. Over an annual cycle, BSi and DSi concentrations varied from 0 to 43.5 μmol L−1 and from 0.3 to 40 μmol L−1, respectively. This was associated with large δ30SiDSi variations from +0.49 ± 0.22‰ (2sd) in spring to +2.92 ± 0.14‰ in winter, which opposed to observations that summer δ30SiDSi values were usually higher than those in winter. This atypical variation could be attributed to the water‐sediment regulation on the Yellow River occurring every early summer, leading to a strong water mixing pattern and suppressing diatom production in summer. This mixing was further prolonged by extreme autumn rainfall on land. The pulse supply of nutrients subsequently enhanced primary productivity from autumn through winter. In spring, the resuspended seafloor sediments were likely an important DSi source with δ30Si values of <−0.5‰. Our findings suggest that natural Si seasonality has been greatly masked by human activities and climate events in the Bohai Sea. Our study serves as a reference of the Si cycle research endeavors worldwide for revealing the overlaying effect of anthropogenic consequences and natural variability.
Chemistry, Growth, and Fate of the Unique, Short‐Lived (2019–2020) Water Lake at the Summit of Kīlauea Volcano, Hawaii
Authors: Nadeau P. A. , Hurwitz S. , Peek S. , Lerner A. H. , Younger E. F. , Patrick M. R. , Damby D. E. , McCleskey R. B. , Kelly P. J.
Published: March 2024

Less than a year after the 2018 Kīlauea caldera collapse and eruption, water appeared in newly deepened Halemaʻumaʻu crater. The lake—unprecedented in the written record—grew to a depth of ∼50 m before lava from the December 2020 eruption boiled it away. Surface water heightened concerns of potential phreatic or phreatomagmatic explosions but also offered a new means of possibly identifying eruption precursors. The U.S. Geological Survey Hawaiian Volcano Observatory (HVO) monitored the lake via direct visual observation, webcams, thermal imaging, colorimetry, and laser rangefinders. HVO also employed uncrewed aircraft systems to sample the water and measure near‐lake gas composition. The lake's δD and δ18O indicate a groundwater source with substantial evaporation. The initial sample had a salinity (total dissolved solids concentration) of 71,000 mg/L and was rich in sulfate (∼53,000 mg/L), iron (∼500 mg/L), and magnesium (∼10,000 mg/L). Subsequent samples were slightly more dilute. The water's pH (∼4), δ34S (+4.3‰), and surface temperatures (up to 85°C) suggest, rather than significant scrubbing of magmatic volatiles, leaching of basalt and reactions with sulfate minerals resulted in high concentrations of sulfate and other solutes. Thermodynamic modeling and precipitate mineralogy indicate that water composition was controlled by iron oxidation and sulfate dissolution. Although the lake exhibited no detectable precursors before the next eruption, and phreatic or phreatomagmatic explosions did not materialize, our multi‐parameter approach to monitoring yielded an enhanced understanding of the hydrologic, geologic, and magmatic conditions that led to the formation of the unique and short‐lived lake.
Hybrid Theory‐Guided Data Driven Framework for Calculating Irrigation Water Use of Three Staple Cereal Crops in China
Authors: Bo Yong , Li Xueke , Liu Kai , Wang Shudong , Li Dehui , Xu Yu , Wang Mengmeng
Published: March 2024

Current irrigation water use (IWU) estimation methods confront uncertainties warranting further attention, primarily stemming from constraints within model structure and data quality. This study proposes a hybrid framework that integrates multiple machine learning (ML) methods with theory‐guided strategies to calculate IWU for three principal cereal crops within the Chinese agricultural landscape. We generated high resolution time series data sets of evapotranspiration and surface soil moisture (SM) using remote sensing resources. ML techniques, along with the Bayesian three‐cornered hat ensemble, were employed to drive multiple remote sensing‐derived data sets in IWU calculation. We applied two theory‐guided mechanisms to quantify irrigation signals: first, converting original SM values into logarithmic terms, and second, extracting process‐based SM residuals. Proposed framework has been validated at 12 field stations across China, yielding coefficient of determination (R2) ranging from 0.54 to 0.70, and root mean square error (RMSE) spanning 278–335 mm/yr. Our framework demonstrates considerable strength in IWU estimation when compared to reported IWU values form 341 cities across China. Specifically, for rice, wheat, and maize, the R2 values range from 0.78 to 0.83, 0.68 to 0.76, and 0.53 to 0.64, respectively, with corresponding RMSE measuring 0.22–0.25, 0.10–0.12, and 0.11–0.13 km3/yr, respectively. These findings highlight the effectiveness of theory‐guided strategies in discerning irrigation‐related information, thereby improving overall model performance. Attention should be directed toward the uncertainties in evapotranspiration and precipitation products on model performance, which remained modest, with a relative change of less than 5%.
The HIF-1α/PLOD2 axis integrates extracellular matrix organization and cell metabolism leading to aberrant musculoskeletal repair
Authors: Kang Heeseog , Strong Amy L. , Sun Yuxiao , Guo Lei , Juan Conan , Bancroft Alec C. , Choi Ji Hae , Pagani Chase A. , Fernandes Aysel A. , Woodard Michael , Lee Juhoon , Ramesh Sowmya , James Aaron W. , Hudson David , Dalby Kevin N. , Xu Lin , Tower Robert J. , Levi Benjamin
Published: March 2024

While hypoxic signaling has been shown to play a role in many cellular processes, its role in metabolism-linked extracellular matrix (ECM) organization and downstream processes of cell fate after musculoskeletal injury remains to be determined. Heterotopic ossification (HO) is a debilitating condition where abnormal bone formation occurs within extra-skeletal tissues. Hypoxia and hypoxia-inducible factor 1α (HIF-1α) activation have been shown to promote HO. However, the underlying molecular mechanisms by which the HIF-1α pathway in mesenchymal progenitor cells (MPCs) contributes to pathologic bone formation remain to be elucidated. Here, we used a proven mouse injury-induced HO model to investigate the role of HIF-1α on aberrant cell fate. Using single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics analyses of the HO site, we found that collagen ECM organization is the most highly up-regulated biological process in MPCs. Zeugopod mesenchymal cell-specific deletion of Hif1α (Hoxa11-CreERT2; Hif1afl/fl) significantly mitigated HO in vivo. ScRNA-seq analysis of these Hoxa11-CreERT2; Hif1afl/fl mice identified the PLOD2/LOX pathway for collagen cross-linking as downstream of the HIF-1α regulation of HO. Importantly, our scRNA-seq data and mechanistic studies further uncovered that glucose metabolism in MPCs is most highly impacted by HIF-1α deletion. From a translational aspect, a pan-LOX inhibitor significantly decreased HO. A newly screened compound revealed that the inhibition of PLOD2 activity in MPCs significantly decreased osteogenic differentiation and glycolytic metabolism. This suggests that the HIF-1α/PLOD2/LOX axis linked to metabolism regulates HO-forming MPC fate. These results suggest that the HIF-1α/PLOD2/LOX pathway represents a promising strategy to mitigate HO formation.
RNA expression of 6 genes from metastatic mucosal gastric cancer serves as the global prognostic marker for gastric cancer with functional validation
Authors: Suh Yun-Suhk , Lee Jieun , George Joshy , Seol Donghyeok , Jeong Kyoungyun , Oh Seung-Young , Bang Chanmi , Jun Yukyung , Kong Seong-Ho , Lee Hyuk-Joon , Kim Jong-Il , Kim Woo Ho , Yang Han-Kwang , Lee Charles
Published: March 2024

Background

Molecular analysis of advanced tumors can increase tumor heterogeneity and selection bias. We developed a robust prognostic signature for gastric cancer by comparing RNA expression between very rare early gastric cancers invading only mucosal layer (mEGCs) with lymph node metastasis (Npos) and those without metastasis (Nneg).

Methods

Out of 1003 mEGCs, all Npos were matched to Nneg using propensity scores. Machine learning approach comparing Npos and Nneg was used to develop prognostic signature. The function and robustness of prognostic signature was validated using cell lines and external datasets.

Results

Extensive machine learning with cross-validation identified the prognostic classifier consisting of four overexpressed genes (HDAC5, NPM1, DTX3, and PPP3R1) and two downregulated genes (MED12 and TP53), and enabled us to develop the risk score predicting poor prognosis. Cell lines engineered to high-risk score showed increased invasion, migration, and resistance to 5-FU and Oxaliplatin but maintained sensitivity to an HDAC inhibitor. Mouse models after tail vein injection of cell lines with high-risk score revealed increased metastasis. In three external cohorts, our risk score was identified as the independent prognostic factor for overall and recurrence-free survival.

Conclusion

The risk score from the 6-gene classifier can successfully predict the prognosis of gastric cancer.
Suppression of apoptosis impairs phalangeal joint formation in the pathogenesis of brachydactyly type A1
Authors: Leung Adrian On Wah , Poon Andrew Chung Hin , Wang Xue , Feng Chen , Chen Peikai , Zheng Zhengfan , To Michael KaiTsun , Chan Wilson Cheuk Wing , Cheung Martin , Chan Danny
Published: March 2024

Apoptosis occurs during development when a separation of tissues is needed. Synovial joint formation is initiated at the presumptive site (interzone) within a cartilage anlagen, with changes in cellular differentiation leading to cavitation and tissue separation. Apoptosis has been detected in phalangeal joints during development, but its role and regulation have not been defined. Here, we use a mouse model of brachydactyly type A1 (BDA1) with an IhhE95K mutation, to show that a missing middle phalangeal bone is due to the failure of the developing joint to cavitate, associated with reduced apoptosis, and a joint is not formed. We showed an intricate relationship between IHH and interacting partners, CDON and GAS1, in the interzone that regulates apoptosis. We propose a model in which CDON/GAS1 may act as dependence receptors in this context. Normally, the IHH level is low at the center of the interzone, enabling the “ligand-free” CDON/GAS1 to activate cell death for cavitation. In BDA1, a high concentration of IHH suppresses apoptosis. Our findings provided new insights into the role of IHH and CDON in joint formation, with relevance to hedgehog signaling in developmental biology and diseases.
Speaking without vocal folds using a machine-learning-assisted wearable sensing-actuation system
Authors: Che Ziyuan , Wan Xiao , Xu Jing , Duan Chrystal , Zheng Tianqi , Chen Jun
Published: March 2024

Voice disorders resulting from various pathological vocal fold conditions or postoperative recovery of laryngeal cancer surgeries, are common causes of dysphonia. Here, we present a self-powered wearable sensing-actuation system based on soft magnetoelasticity that enables assisted speaking without relying on the vocal folds. It holds a lightweighted mass of approximately 7.2 g, skin-alike modulus of 7.83 × 105 Pa, stability against skin perspiration, and a maximum stretchability of 164%. The wearable sensing component can effectively capture extrinsic laryngeal muscle movement and convert them into high-fidelity and analyzable electrical signals, which can be translated into speech signals with the assistance of machine learning algorithms with an accuracy of 94.68%. Then, with the wearable actuation component, the speech could be expressed as voice signals while circumventing vocal fold vibration. We expect this approach could facilitate the restoration of normal voice function and significantly enhance the quality of life for patients with dysfunctional vocal folds.
Policy and market forces delay real estate price declines on the US coast
Authors: McNamara Dylan E. , Smith Martin D. , Williams Zachary , Gopalakrishnan Sathya , Landry Craig E.
Published: March 2024

Despite increasing risks from sea-level rise (SLR) and storms, US coastal communities continue to attract relatively high-income residents, and coastal property values continue to rise. To understand this seeming paradox and explore policy responses, we develop the Coastal Home Ownership Model (C-HOM) and analyze the long-term evolution of coastal real estate markets. C-HOM incorporates changing physical attributes of the coast, economic values of these attributes, and dynamic risks associated with storms and flooding. Resident owners, renters, and non-resident investors jointly determine coastal property values and the policy choices that influence the physical evolution of the coast. In the coupled system, we find that subsidies for coastal management, such as beach nourishment, tax advantages for high-income property owners, and stable or increasing property values outside the coastal zone all dampen the effects of SLR on coastal property values. The effects, however, are temporary and only delay precipitous declines as total inundation approaches. By removing subsidies, prices would more accurately reflect risks from SLR but also trigger more coastal gentrification, as relatively high-income owners enter the market and self-finance nourishment. Our results suggest a policy tradeoff between slowing demographic transitions in coastal communities and allowing property markets to adjust smoothly to risks from climate change.
RNA modulates hnRNPA1A amyloid formation mediated by biomolecular condensates
Authors: Morelli Chiara , Faltova Lenka , Capasso Palmiero Umberto , Makasewicz Katarzyna , Papp Marcell , Jacquat Raphaël P. B. , Pinotsi Dorothea , Arosio Paolo
Published: March 2024

Several RNA binding proteins involved in membraneless organelles can form pathological amyloids associated with neurodegenerative diseases, but the mechanisms of how this aggregation is modulated remain elusive. Here we investigate how heterotypic protein–RNA interactions modulate the condensation and the liquid to amyloid transition of hnRNPA1A, a protein involved in amyothropic lateral sclerosis. In the absence of RNA, formation of condensates promotes hnRNPA1A aggregation and fibrils are localized at the interface of the condensates. Addition of RNA modulates the soluble to amyloid transition of hnRNPA1A according to different pathways depending on RNA/protein stoichiometry. At low RNA concentrations, RNA promotes both condensation and amyloid formation, and the catalytic effect of RNA adds to the role of the interface between the dense and dilute phases. At higher RNA concentrations, condensation is suppressed according to re-entrant phase behaviour but formation of hnRNPA1A amyloids is observed over longer incubation times. Our findings show how heterotypic nucleic acid–protein interactions affect the kinetics and molecular pathways of amyloid formation.
Remnants of shifting early Cenozoic Pacific lower mantle flow imaged beneath the Philippine Sea Plate
Authors: Fan Jianke , Zhao Dapeng , Li Cuilin , Liu Lijun , Dong Dongdong
Published: March 2024

Seismic anisotropy could provide vital information about the evolution and internal convection of the deep Earth interior. Although previous seismological studies have revealed a wide distribution of seismic anisotropy in the upper portion of the lower mantle beneath many subduction zones, the existence of anisotropy at these depths away from subducted slabs remains debated. Here we use P-wave azimuthal anisotropy tomography to image the crust and mantle down to 1,600-km depth. We find prominent anisotropic patterns in the upper portion of the lower mantle beneath the Philippine Sea Plate. Substantial azimuthal anisotropy with N–S fast-velocity directions occurs at 700–900-km depths. We interpret this azimuthal anisotropy as a remnant of the Pacific lower mantle flow field about 50 million years ago. Two isolated high-velocity anomalies at 700–1,600-km depths may be vestigial pieces of the subducted Izanagi slab with seismic velocity features suggesting a shift in the Pacific lower mantle flow field by about 40 million years ago. Our findings provide seismic evidence for the existence of complex lower mantle flows and deformation mechanisms away from subduction zones.
Classification and automatic scoring of arousal intensity during sleep stages using machine learning
Authors: Han Hyewon , Seong Min Jae , Hyeon Janghun , Joo Eunyeon , Oh Junhyoung
Published: March 2024

Arousal during sleep can result in sleep fragmentation and various physiological effects, impairing cognitive function and raising blood pressure and heart rate. However, the current definition of arousal has limitations in assessing both amplitude and duration, making it challenging to measure sleep fragmentation accurately. Moreover, there is inconsistency among inter-raters in arousal scoring, which renders it susceptible to subjective variability. Therefore, this study aims to identify a highly accurate classifier for each sleep stage by employing optimized feature selection and machine learning models. According to electroencephalography (EEG) signals during the arousal phase, the intensity level was categorized into four levels. For control, the non-arousal cases were used as level 0 and referred as sham arousal, resulting in five arousal intensity levels. Wavelet transform was applied to analyze sleep arousal to extract features from EEG. Based on these features, we classified arousal intensity levels through machine learning algorithms. Due to the different characteristics of EEG in each sleep stage, the classification model was optimized for the four sleep stages. Excluding sham arousals, a total of 13,532 arousal events were used. The lowest intensity in the entire data, level 1, was computed to be 3107, level 2 was 3384, level 3 was 3472, and the highest intensity of level 4 was 3,569. The optimized classification model for each sleep stage achieved an average sensitivity of 82.68%, specificity of 95.68%, and AUROC of 96.30%. The sensitivity of the control, arousal intensity level 0, was 83.07%, a 1.25% increase over the unoptimized model and a 14.22% increase over previous research. This study used machine learning techniques to develop classifiers for each sleep stage, improving the accuracy of arousal intensity classification. The classifiers showed high sensitivity and specificity and revealed the unique characteristics of arousal intensity during different sleep stages. These findings represent a novel approach to arousal research and have implications for developing more accurate predictive models in sleep research.
Compliance with Australian Orthopaedic Association guidelines does not reduce the risk of venous thromboembolism after total hip and knee arthroplasty
Authors: Badge Helen , Churches Tim , Naylor Justine M. , Xuan Wei , Armstrong Elizabeth , Gray Leeanne , Fletcher John , Gosbell Iain , Lin Chung-Wei Christine , Harris Ian A.
Published: March 2024

Preventing avoidable venous-thrombo-embolism (VTE) is a priority to improve patient and service outcomes after total hip and total knee arthroplasty (THA, TKA), but compliance with relevant clinical guidelines varies. This study aims to determine the degree to which prophylaxis was compliant with Australian Orthopaedic Association (AOA) VTE prophylaxis guidelines and whether non-compliance is associated with increased risk of VTE. A prospective multi-centre cohort study of adults with osteoarthritis undergoing primary TKA/THA was completed at 19 high-volume public and private hospitals. Data were collected prior to surgery and for one-year post-surgery. Logistic regression was undertaken to explore associations between non-compliance with AOA VTE prophylaxis guidelines and symptomatic 90-day VTE outcomes. Data were analysed for 1838 participants from 19 sites. The rate of non-compliance with all clinical guideline recommendations was 20.1% (N = 369), with 14.1% (N = 259) non-compliance for risk-stratified prophylaxis, 35.8% (N = 658) for duration, and 67.8% (N = 1246) for other general recommendations. Symptomatic VTE was experienced up to 90-days post-surgery by 48 people (2.6%). Overall guideline non-compliance (AOR = 0.93, 95%CI = 0.4 to 1.3, p = 0.86) was not associated with a lower risk of symptomatic 90-day VTE. Results were consistent when people with high bleeding risk were excluded (AOR = 0.94, 95%CI = 0.44 to 2.34, p = 0.89). Non-compliance with the AOA VTE prophylaxis guidelines was not associated with risk of 90-day VTE after arthroplasty. This counterintuitive finding is concerning and necessitates a rigorous review of the AOA VTE prevention clinical guideline.
Hybrid similarity relation based mutual information for feature selection in intuitionistic fuzzy rough framework and its applications
Authors: Tiwari Anoop Kumar , Saini Rajat , Nath Abhigyan , Singh Phool , Shah Mohd Asif
Published: March 2024

Fuzzy rough entropy established in the notion of fuzzy rough set theory, which has been effectively and efficiently applied for feature selection to handle the uncertainty in real-valued datasets. Further, Fuzzy rough mutual information has been presented by integrating information entropy with fuzzy rough set to measure the importance of features. However, none of the methods till date can handle noise, uncertainty and vagueness simultaneously due to both judgement and identification, which lead to degrade the overall performances of the learning algorithms with the increment in the number of mixed valued conditional features. In the current study, these issues are tackled by presenting a novel intuitionistic fuzzy (IF) assisted mutual information concept along with IF granular structure. Initially, a hybrid IF similarity relation is introduced. Based on this relation, an IF granular structure is introduced. Then, IF rough conditional and joint entropies are established. Further, mutual information based on these concepts are discussed. Next, mathematical theorems are proved to demonstrate the validity of the given notions. Thereafter, significance of the features subset is computed by using this mutual information, and corresponding feature selection is suggested to delete the irrelevant and redundant features. The current approach effectively handles noise and subsequent uncertainty in both nominal and mixed data (including both nominal and category variables). Moreover, comprehensive experimental performances are evaluated on real-valued benchmark datasets to demonstrate the practical validation and effectiveness of the addressed technique. Finally, an application of the proposed method is exhibited to improve the prediction of phospholipidosis positive molecules. RF(h2o) produces the most effective results till date based on our proposed methodology with sensitivity, accuracy, specificity, MCC, and AUC of 86.7%, 90.1%, 93.0% , 0.808, and 0.922 respectively.