bluuebunny/crossref_metadata_2025 huggingface dataset:
Pengaruh Pemberian Pendidikan Kesehatan Terhadap Tingkat Pengetahuan Tentang Cardiac Arrest Dan Bantuan Hidup Dasar Tim Komunitas Gabungan Emergency
Authors: Khalilati Noor , Ruslinawati Ruslinawati , Nazarudin Akhmad
Published: December 2022

Latar Belakang: Kurangnya pengetahuan masyarakat tentang Cardiac Arrest dan Bantuan Hidup Dasar dalam melakukan pertolongan kegawatdaruratan sehingga pengetahuan masyarakat tentang Cardiac Arrest dan Bantuan Hidup Dasar dirasa perlu dikaji karena kondisi gawat darurat dapat terjadi kapan saja dan dimana saja.Tujuan: Untuk mengetahui pengaruh pemberian pendidikan kesehatan tentang cardiac arrest dan bantuan hidup dasar terhadap tingkat pengetahuan Tim Komunitas Gabungan Emergency (KGE).Metode: Desain penelitian yaitu quasy experiment with one group pre-posttest dan menggunakan lembar kuesioner untuk mendapatkan data dari responden. Sampel berjumlah 30 responden dengan menggunakan teknik probability sampling.Hasil: Penelitian didapatkan rata-rata nilai pretest 72,33 dan nilai posttest 87,50 dan di dapat nilai p-value = 0,000 (α 0,05).Simpulan:Terdapat pengaruh pendidikan kesehatan tentang Cardiac Arrest dan Bantuan Hidup Dasar terhadap tingkat pengetahuan Tim Komunitas Gabungan Emergency HSU. Peranan relawan sebagai penemu pertama sangat berpengaruh. Sehingga dirasa perlu untuk mengikuti pendidikan kesehatan berupa seminar atau pelatihan yang berkaitan dengan Cardiac Arrest dan Bantuan Hidup Dasar secara berkala tiap tahun. Kata Kunci: Pengetahuan, Cardiac Arrest, Bantuan Hidup Dasar, Komunitas Gabungan emergency (KGE) The Effect of Providing Health Education on the Level of Knowledge About Cardiac Arrest and Basic Life Support Emergency Community TeamBackground: Public acknowledgement about Cardiac Arrest and Basic Life Support in providing emergency assistance so that public knowledge about Cardiac Arrest and Basic Life Support is deemed necessary because emergency conditions can occur anytime and anywhereObjective: To determine the effect of providing health education about cardiac arrest and basic life support on the level of knowledge of the KGE TeamMethods: This research using a quasi-experiment design with one group pre-posttest and uses a questionnaire sheet to obtain data from respondents. 30 respondents sample use for this research with probability sampling techniqueResult: This research obtained an average pretest value of 72.33 and a posttest value of 87.50 and the p-value = 0.000 (α 0.05)Conclusion: There is an effect of health education on Cardiac Arrest and Basic Life Support on the level of knowledge of the HSU Emergency Joint Community Team. The role of volunteers as the first inventors is very influential. It is necessary to attend health education in the form of seminars or training related to Cardiac Arrest and Basic Life Support on a regular basis every year. Keywords: Knowledge, Cardiac Arrest, BLS, KGE Team
Efektifitas Metode Pengkajian Siriraj Stroke Score (SSS) dan National Institute of Health Stroke Scale (NIHSS) dalam Penetapan Diagnosa Keperawatan Aktual pasien Stroke di Ruang IGD
Authors: Mohtar M. Sobirin , Rahman Subhannur , Apriannor Ahmad , Auliyah Gusti Restu
Published: December 2022

Latar Belakang: Stroke adalah penyakit pada otak berupa gangguan fungsi syaraf fokal disebabkan oleh terganggunya peredaran darah pada otak dan menimbulkan gejala-gejala berupa defisit neurologis. Prevalensi stroke di Indonesia mengalami kenaikan dari 7% menjadi 10,9%. Provinsi Kalimantan selatan menempati urutan ketiga dengan prevalensi 12,7 % permil. Seiring meningkatnya jumlah penderita stroke, perlu pengkajian berfokus pada neuorologi dan bersifat darurat.Tujuan : Penelitian ini bertujuan untuk menganalisis efektifitas metode pengkajian sss dan nihss dalam penetapan jumlah diagnosa keperawatan aktual dan kecepatan waktu penetapan diagnosa pasien stroke di Ruang IGD.Metode : Penelitian ini menggunakan pendekatan pra-eksperimen (the one shot case study).Hasil : Hasil penelitian menggunakan analisis uji korelasi sperman pada metode SSS menunjukkan durasi pengkajian p=0,000, jumlah diagnosa p=0,000 dan durasi penetapan p=0,000 Î± (0,05). Sedangkan NIHSS menunjukkan durasi pengkajian p=0,000, jumlah diagnosa p=0,887 dan durasi penetapan p=0,000 Î± (0,05). Sehingga ada hubungan metode pengkajian SSS dan NIHSS terhadap penetapan diagnosa keperawatan aktual. Sedangkan hasil tingkat keeratan yang dianalisis menggunakan koefisien korelasi bahwa terdapat hubungan yang sangat kuat adalah metode NIHSS terhadap lama durasi penetapan (r=0,858). Terdapat hubungan yang sama kuatnya adalah metode SSS dan NIHSS terhadap lama durasi pengkajian (r=0,653) namun lebih tinggi nilai SSS (r=0,696) sedangkan lama durasi penetapan SSS (r=0,696). Terdapat hubungan yang cukup adalah metode SSS terhadap jumlah diagnosa yang ditetapkan (r=0,495). Tidak terdapat hubungan, sehingga hubungan menjadi tidak berarti terjadi pada metode NIHSS terhadap jumlah diagnosaÂ  (r=-0,027).Kesimpulan : Metode pengkajian SSS dan NIHSS efektif dalam menetapkan diagnosa keperawatan aktual pada pasien stroke di Ruang IGD, namun secara durasi pengkajian dan penetapan jumlah diagnosa lebih efektif SSS. sedangkan dari durasi penetapan diagnosa keperawatan lebih efektif NIHSS.
HUBUNGAN KEHADIRAN KELUARGA DENGAN TINGKAT KECEMASAN PERAWAT SAAT RESUSITASI DI RUMAH SAKIT BANJARMASIN
Authors: Luqyana Atika , Julianto Julianto
Published: December 2022

A life-saving medical action can bring anxiety, because there is a threat of bodily integrity, emergency response measures always prioritize patient safety, where the presence of a patient's family who sees a nurse's actions while taking a nurse's resuscitation must act quickly and eventually feel anxious caused by anticipation of misconduct. This study aimed to determine the relationship of nurse anxiety levels when resuscitation with the presence of the family beside the patient. This study used a cross sectional design. The population is all nurses who are in the ICU with a sample taken with a total sampling technique of 30 people. Data analysis using Spearman rank. The results of the study found that most nurses experienced mild anxiety, there was a family relationship with the anxiety level of nurses when resuscitation in the ICU Hospital Dr. H. Moch Ansari Saleh Banjarmasin with the strength of a moderate relationship. It is expected that as nurses who will carry out nursing actions can overcome and reduce the sense of anxiety of nurses.

Suatu tindakan medis menyelamatkan jiwa dapat mendatangkan kecemasan, karena terdapat ancaman integritas tubuh, tindakan penanggulangan kegawatdaruratan selalu mengutamakan keselamat pasien, dimana kehadiran keluarga pasien yang melihat tindakan perawat saat melakukan tindakan resusitasi perawat harus bertindak cepat dan akhirnya cenderung merasa cemas yang disebabkan oleh antisipasi terhadap kesalahan tindakan. Penelitian ini bertujuan untuk mengetahui hubungan tingkat kecemasan perawat saat tindakan resusitasi dengan kehadiran keluarga di samping pasien. Penelitian ini menggunakan desain cross sectional. Populasi adalah semua perawat yang berada di Ruang ICU dengan sampel diambil dengan tehnik total sampling berjumlah 30 orang. Analisis data menggunakan spearman rank. Hasil didapatkan bahwa sebagian besar perawat mengalami kecemasan ringan, ada hubungan kehadiran keluarga dengan tingkat kecemasan perawat saat tindakan resusitasi di Ruang ICU RSUD Dr. H. Moch Ansari Saleh Banjarmasin dengan kekuatan hubungan yang sedang. Diharapkan Sebagai perawat yang akan melakukan tindakan keperawatan dapat mengatasi serta mengurangi rasa kecemasan perawat.
HUBUNGAN BODYSHAMING DENGAN KONSEP DIRI REMAJA DIPONDOK PESANTREN DARUL HIJRAH PUTRI MARTAPURA
Authors: Anwari Muhammad , Hafizah Annisa
Published: December 2022

Background: The incidence of stroke is quite high each year and the problem can have to more serious Body shaming is an act or practice of trusting someone by making derogatory comments or criticizing their body shape or size when discussing themselves, which includes one's perception of themselves, feelings, beliefs, and values ​​related to themselves. Adolescence is the most important period of the environment and peers, it is the factors that influence its formation, the attitude towards self-concept which is an important aspect in adolescents. The existence of interactions to the search for self-identity in adolescents will certainly be found in things that are negative or undesirable. For example, from peers, who carry out acts of intimidation to self-harm adolescents will certainly affect the process of development. Objective: The purpose of this study was to study the relationship with teenagers at Darul Hijrah Putri Martapura Islamic Boarding School. Method: This research design uses Cross-Sectional and uses the Pearson Product Moment statistical test. The sample was determined by a Simple Random Sampling technique with 157 people out of 258 people. Result: The results of statistical analysis of the relationship between body shaming with self-concept get a value of 0.657 meaning the variable has a strong relationship. Conclusion: There is a relationship between body shaming with self-concept in adolescents at Darul Hijrah Putri Martapura Islamic Boarding School in 2020.

Latar belakang: komentar mengejek atau kritis tentang bentuk atau ukuran tubuh mereka sedangkan konsep diri adalah keseluruhan gambaran diri, yang meliputi persepsi seseorang tentang dirinya, perasaan, keyakinan dan nilai-nilai yang berhubungan dengan dirinya. Masa remaja merupakan masa yang paling banyak dipengaruhi oleh lingkungan dan teman-teman sebaya, itu lah yang menjadi faktor-faktor yang mempengaruhi terbentuknya kepribadian, sikap hingga konsep diri yang menjadi aspek penting dalam diri remaja. Adanya interaksi hingga pencarian identitas diri pada remaja, tentu akan ditemukan hal-hal yang negatif atau yang tidak diinginkan. Misalkan saja dari teman sebaya, yang melakukan tindakan intimidasi hingga merugikan diri remaja tentu itu akan berpengaruh pada proses perkembangannya. Tujuan: Penelitian ini untuk mengetahui hubungan dengan konsep diri pada remaja di Pondok Pesantren Darul Hijrah Putri Martapura. Metode: Desain penelitian ini menggunakan Cross Sectional dan menggunakan uji statistik Pearson Product Moment. Sample ditentukan dengan teknik Simple Random Sampling sebanyak 157 orang dari jumlah 258 orang. Hasil: Hasil analisis statistic hubungan antara body shaming dengan konsep diri mendapatkan hasil nilai korelasi sebesar 0,657 artinya kedua variabel memiliki hubungan yang kuat. Kesimpulan: Ada hubungan antara body shaming dengan konsep diri pada remaja di Sekolah Menengah Atas Pondok Pesantren Darul Hijrah Putri Martapura Tahun 2020.
ANALISIS KARAKTERISTIK PASIEN YANG BERHUBUNGAN DENGAN KUALITAS HIDUP PASIEN GAGAL JANTUNG DI RUMAH SAKIT UMUM DAERAH ULIN BANJARMASIN
Authors: Aprilia Hanura
Published: December 2022

Background: Heart failure disease cause a decreasing of physic and psychologic function, failed in doing nursing principals of client with health failure could decrease the life quality, useless hospitalization and faster death. Objective: The aims of this study was to analyze caractheristics patient related the quality of life of patients with heart failure. Methods: This study used the analytic correlation with cross-sectional design. The number of samples in this study there are 100 respondents. Result: In the result of this study are the characteristic of Respondent have relation to the quality of Life in Patient with Heart Failure in physical functional of the patient with p value 0,000 < α with OR: 10.578 (4.124-27.133)

Latar Belakang: Penyakit Gagal Jantung menyebabkan penurunan fungsi fisik dan psikologis, kegagalan dalam melaksanakan prinsip-prinsip perawatan pasien gagal jantung dapat mengakibatkan kualitas hidup menurun, hospitalisasi yang sia-sia dan kematian lebih cepat. Tujuan: Untuk mengetahui karakteristik responden yang berhubungan dengan kualitas hidup pasien penyakit gagal jantung. Metode: Penelitian ini menggunakan analitik korelasi dengan desain cross sectional. Jumlah sampel dalam penelitian ini ada 100 responden. Hasil: Pada hasil penelitian ini bahwa karakteristik responden yang mempengaruhi kualitas hidup pasien gagal jantung adalah fungsi fisik dengan p-value 0.000 ≤ α dengan OR: 10.578 (4.124-27.133)
PENGARUH TERAPI PIJAT KAKI TERHADAP STATUS HEMODINAMIK PADA PASIEN TERPASANG VENTILATOR DI INTENSIVE CARE UNIT (ICU) RSUD ULIN BANJARMASIN
Authors: Daud Izma , Sari Revina Nurul
Published: December 2022

Ketidakstabilan hemodinamik pasien yang dirawat di ruang Intensive care unit (ICU). Faktor penyebabnya karena stressor yang berasal dari aspek fisiologis, psikologis, maupun lingkungan. Terapi yang diberikan untuk pasien di ICU didominasi oleh terapi farmakologi. Sementara berdasarkan teori keperawatan holistik, asuhan perawatan pada pasien dapat dioptimalkan dengan terapi komplementer seperti foot massage. Rancangan Penelitian menggunakan pra eksperimen dengan metode one group pre-post test design. Sampel penelitian berjumlah 31 responden dengan menggunakan teknik purposive sampling. Pada penelitian dianalisis menggunakan Uji Wilcoxon menunjukkan terdapat pengaruh foot massage secara signifikan terhadap penurunan tekanan darah sistol 0.020 (<0.05), tekanan darah diastol 0.025 (<0.05), MAP 0.013 (<0.05), nadi 0.034 (<0.05), respirasi 0.001(<0.05) namun tidak terdapat pengaruh foot massage secara signifikan terhadap peningkatan saturasi oksigen 0.317 (>0.05). Foot massage dapat memperlancar sirkulasi darah, tekanan pada otot secara bertahap dapat mengendurkan ketegangan sehingga membantu memperlancar aliran darah ke jantung

Hemodynamic instability of patients treated in the Intensive Care Unit (ICU). The cause factor is because of stressors that come from physiological, psychological, and environmental aspects. Therapy given to patients in the ICU is dominated by pharmacological therapy. While based on holistic nursing theory, care for patients can be optimized with complementary therapies such as foot massage. The research design uses pre-experimental with one group pre-post test design method. The research sample of 31 respondents using a purposive sampling technique. In the study analyzed using the Wilcoxon Test showed that there was a significant influence of foot massage on the decrease in systolic blood pressure 0.020 (<0.05), diastolic blood pressure 0.025 (<0.05), MAP 0.013 (<0.05), pulse 0.034 (<0.05), respiration 0.001 ( <0.05) but there was no significant effect of foot massage on increasing oxygen saturation by 0.317 (> 0.05). A Foot massage can accelerate blood circulation, pressure on the muscles can gradually relax the tension so that helps accelerate blood flow to the heart
LITERATURE REVIEW: EFEKTIFITAS TEMULAWAK (CURCUMA XANTHORRIZA ROBX) TERHADAP PENINGKATAN BERAT BADAN PADA ANAK
Authors: Raziansyah Raziansyah
Published: December 2022

Latar Belakang: Di Indonesia prevalensi gizi kurang dan gizi buruk anak sebanyak 19,6%, hal ini menjadi tantangan besar sehingga memerlukan perhatian dalam peningkatan konsumsi makan pada anak, sebagai indikatornya adalah berat badan. Salah satu cara adalah dengan komsumsi jamu herbal yaitu temulawak yang secara empiris banyak digunakan sebagai obat tunggal maupun campuran untuk meningkatkan nafsu makan dan berat badan. Tujuan penelitian ini adalah untuk mengetahui efektifitas temulawak (curcuma xanthorriza robx) terhadap peningkatan berat badan pada anak. Metode: Database dikumpulkan melalui penelusuran google scholar, data dikumpulkan dalam kurun waktu tahun 2015-2020 dengan kriteria inklusi penelitian dengan responden anak usia dibawah 9 tahun. Hasil: Didapatkan data 3 artikel dari 53 hasil penelusuran. Kesimpulan: Rimpang temulawak merupakan bahan pembuatan obat tradisional yang paling utama. Khasiat temulawak secara empiris sebagai peningkat aktivitas nafsu makan dan berat badan anak, selain itu juga temulawak juga dapat meningkatkan sistem kekebalan tubuh, Temulawak juga memiliki efek farmakologis zat aktif sebagai anti-inflamasi (anti peradangan) dan menghambat edema (pembengkakan).
HUBUNGAN KOMUNIKASI INTERPROFESIONAL DENGAN KOLABORASI PERAWAT-DOKTER DI IRNA RSUD H. DAMANHURI BARABAI
Authors: Murdiany Noor Anna
Published: December 2022

Latar Belakang: Komunikasi interprofessional adalah keterampilan penting yang dapat meningkatkan fungsi tim yang berkualitas tinggi, dalam perawatan pasien dengan  melibatkan beberapa disiplin ilmu seperti dokter, perawat dan tim kesehatan lainnya. Kolaborasi merupakan sebuah bentuk kerjasama antar tim tenaga kesehatan dari latar belakang pendidikan yang berbeda-beda untuk memberikan kualitas pelayanan yang terbaik kepada pasien dan keluarga pasien.

Tujuan: Mengetahui hubungan komunikasi interprofesional dengan kolaborasi perawat-dokter di IRNA RSUD H. Damanhuri Barabai.

Metode: Desain penelitian menggunakan cross sectional dengan teknik total sampling. Instrumen penelitian menggunakan kuesioner komunikasi interprofesional dan kolaborasi perawat-dokter yang sudah di uji validitas dan reliabilitas. Data dianalisis menggunakan uji Chi Square.

Kesimpulan: Ada hubungan komunikasi interprofesional dengan kolaborasi perawat-dokter Di IRNA RSUD H. Damanhuri Barabai. Diharapkan perawat dan dokter meningkatkankan kemampuan dalam berkomunikasi dan berkolaborasi guna meningkatkan mutu layanan kesehatan.

Kata kunci: Komunikasi Interprofesional, Kolaborasi, perawat-dokter.
EFEKTVITAS TERAPI AIR PUTIH TERHADAP KADAR GULA DARAH PADA PASIEN DIABETES MELITUS TIPE 2 STUDI: NARRATIVE REVIEW
Authors: Hikmah Noor , Tasalim Rian , Dhea Manto Onieqie Ayu
Published: December 2022

Latar Belakang: Diabetes melitus merupakan penyakit yang ditandai dengan tingginya gula darah. Salah satu cara pengendalian kenaikan gula darah pada pasien diabetes melitus yaitu dengan Hydroterapy. Hydroterapy ini dilakukan untuk pemecahan gula dalam darah dan membuang racun melalui keringat dan urin. Penelitian ini bertujuan menganalisis efektivitas hydroterapy dengan kadar gula pada pasien diabetes melitus.

Metode: Rancangan penelitian narrative review. Pencarian narrative mengunakan database yaitu Google Scholar, Pubmed, Elsevier, Biomed Central, dan Portal Garuda. Kriteria jurnal yang digunakan disaring berdasarkan judul literatur, abstrak dan kata kunci atau keyword yaitu Hydroterapy , kadar gula, diabetes melitus tipe 2.

Hasil: Telaah dari 8 jurnal menunjukkan bahwa terapi minum air putih efektif menurunkan kadar gula dalam darah pasien diabetes melitus tipe 2

Kesimpulan: Narrative ini menunjukan bahwa dapat meningkatkan pengetahuan pasien diabetes melitus tipe 2 dengan memberikan informasi mengenai manfaat dari konsumsi air putih yang cukup sebagai terapi kompelementer.
PENGARUH PEMBERIAN PENDIDIKAN KESEHATAN PROGRAM ISI PIRINGKU TERHADAP TINGKAT PENGETAHUAN IBU HAMIL
Authors: Suwandewi Alit , Hiryadi Hiryadi , Rahayu Miranti
Published: December 2022

Status gizi adalah ekspresi suatu keadaan keseimbangan dalam bentuk variable tertentu atau perwujudan dari nutriture dalam bentuk variable tertentu. Status gizi sangatlah penting untuk ibu hamil karena berpengaruh sangat besar terhadap perkembang dan pertumbuhan janin. Tujuan penelitian ini untuk mengetahui pengaruh dari pemberian pendidikan kesehatan program isi piring terhadap tingkat pengetahuan ibu hamil di wilayah kerja puskesmas pekauman. Metode penelitian ini adalah Pra Experimental design menggunakan rancangan One Group Pre test - Post test dilakukan pada bulan juni 2021 dengan intervensi pemberian pendidikan kesehatan program isi piringku. Data di ambil menggunakan kuesioner dengan dianalisis dengan menggunakan Uji Statistic Wilcoxon Test. Sampel dalam penelitian ini sebanyak 65 ibu hamil di Wilayah Kerja Puskesmas Pekauman. Teknik pengambilan sampel adalah purposive sampling. Hasil dari penelitian pada tingkat pengatahuan ibu hamil sebelum dan sesudah didapat nilai P value 0.000 < 0.05 dengan demikian dapat disimpulkan bahwa terdapat pengaruh pemberian pendidikan kesehatan program isi piringku terhadap tingkat pengetahuan ibu hamil. Ibu hamil diharapkan dapat meningkatkan informasi terkait program gizi seimbang (isi piringku) dan memahami mengenai porsi makan sehari agar ibu dan bayi sehat dan dapat mencegah anemia pada kehamilan. Bagi pelayanan kesehatan diharapkan untuk lebih meningkatkan pelayanan kesehatan khususnya menyediakan pendidikan kesehatan program isi piringku bagi ibu hamil
HUBUNGAN ANTARA TINGKAT PENGETAHUAN DAN MOTIVASI PERAWAT TERHADAP PELAKSANAAN EWS DI RSUD X
Authors: . Julianto
Published: December 2022

Latar Belakang: Kematian yang tinggi di rumah sakit dapat dicegah dengan pertolongan cepat dan tepat. Di dunia telah diperkenalkan sistem skoring pendeteksian dini dengan penerapan Early Warning Score (EWS). Keberhasilan EWS dalam menurunkan angka kejadian henti jantung dipengaruhi oleh implementasi yang baik dari instrumen EWS. Implementasi yang baik dipengaruhi beberapa faktor diantaranya pengetahuan dan motivasi. Tujuan: Tujuan penelitian yaitu menganalisis hubungan antara tingkat pengetahuan dan motivasi perawat terhadap pelaksanaan EWS di Rumah Sakit. Metode: Penelitian ini menggunakan metode Cross Sectional, populasi perawat rawat inap Rumah Sakit, jumlah sampel 43 orang, Teknik Total Sampling. Instrumen kuesioner dan lembar observasi dan menggunakan uji Spearman Rank. Hasil: Hasil didapatkan tingkat pengetahuan baik 31 orang (72%), motivasi perawat cukup (93,1%) dan pelaksanaan EWS baik dan cukup 40 orang (95,4%). Ada hubungan antara tingkat pengetahuan dan motivasi perawat terhadap pelaksanaan EWS di Rumah Sakit. Tingkat pengetahuan P = 0,018 (p< α) dengan nilai r=0,358, tingkat motivasi P=0,042 (p< α) dengan nilai r=0,312. Kesimpulan: Pemberian pelatihan EWS secara berkala dan juga pemberian reward penghargaan maupun peningkatan insentif bagi perawat, sangat baik untuk meningkatkan pengetahuan dan motivasi sehingga pelaksanaan EWS dapat selalu maksimal.
PENGARUH FOCUS GROUP DISCUSSION (FGD) TERHADAP PENGERAPAN 3M (MENCUCI TANGAN, MEMAKAI MASKER DAN MENJAGA JARAK)
Authors: Harun Lukman
Published: December 2022

Latar Belakang: Awal tahun 2020, dunia digemparkan dengan merabaknya virus baru yaitu coronavirus jenis baru (SARS-CoV-2) dan penyakitnya disebut Coronavirus disease 2019 (COVID-19). Diketahui, asal mula virus ini berasal dari  Wuhan, Tiongkok. Masuknya virus corona di Indonesia membawa dampak besar bagi kehidupan masyarakat, penyebaran virus Corona atau COVID-19 di Indonesia harus kita cegah dengan semaksimal mungkin. Salah satu cara utamanya adalah dengan menerapkan perilaku hidup disiplin. Maka, dengan itu kita harus selalu melakukan langkah 3M yaitu memakai masker, mencuci tangan, menjaga jarak, sebagai upaya mencegah sekaligus memutus rantai penularan COVID-19. Salah satu metode dalam melakukan pendidikan kesehatan yaitu dengan focus group discussion (FGD). Untuk mengetahui pengaruh focus group discussion (FGD) terhadap penerapan 3M  (Mencuci Tangan, Memakai Masker dan Menjaga Jarak)

Metode: Desain peneitian ini adalah Quasi Eksperimental dengan populasi masyarakat yang tinggal dikelurahan pemurus Banjarmasin. Sampel penelitian adalah 30 responden Desain penelitian one group pre-test and post-test. Variabel independen Focus Grup Discussion (FGD) dan variabel dependen penerapan 3M dianlisis dengan Uji Wilcoxon. Hasil penelitian menunjukan Hasil uji statistik Wilcoxon menunjukan signifikan yakni sebesar 0,000 yang lebih kecil dari 0,05 sebagai taraf yang telah ditentukan (p < α) dan dapat dinyatakan terdapat pengaruh yang sangat signifikan antara FGD dengan penerapan 3M pada Kelurahan Pemurus Dalam Banjarmasin

Kesimpulan: hasil penelitian ini didapatkan jika ada pengaruh yang sangat signifikan antara FGD dengan penerapan 3M pada Kelurahan Pemurus Dalam Banjarmasin
Sumoylation-deficient phosphoglycerate mutase 2 impairs myogenic differentiation
Authors: Zhang Yi , Beketaev Ilimbek , Ma Yanlin , Wang Jun
Published: December 2022

Phosphoglycerate mutase 2 (PGAM2) is a critical glycolytic enzyme that is highly expressed in skeletal muscle. In humans, naturally occurring mutations in Phosphoglycerate mutase 2 have been etiologically linked to glycogen storage disease X (GSDX). Phosphoglycerate mutase 2 activity is regulated by several posttranslational modifications such as ubiquitination and acetylation. Here, we report that Phosphoglycerate mutase 2 activity is regulated by sumoylation—a covalent conjugation involved in a wide spectrum of cellular events. We found that Phosphoglycerate mutase 2 contains two primary SUMO acceptor sites, lysine (K)49 and K176, and that the mutation of either K to arginine (R) abolished Phosphoglycerate mutase 2 sumoylation. Given that K176 is more highly evolutionarily conserved across paralogs and orthologs than K49 is, we used the CRISPR-mediated homologous recombination technique in myogenic C2C12 cells to generate homozygous K176R knock-in cells (PGAM2K176R/K176R). Compared with wild-type (WT) C2C12 cells, PGAM2K176R/K176R C2C12 cells exhibited impaired myogenic differentiation, as indicated by decreased differentiation and fusion indexes. Furthermore, the results of glycolytic and mitochondrial stress assays with the XF96 Extracellular Flux analyzer revealed a reduced proton efflux rate (PER), glycolytic PER (glycoPER), extracellular acidification rate (ECAR), and oxygen consumption rate (OCR) in PGAM2K176R/K176R C2C12 cells, both at baseline and in response to stress. Impaired mitochondrial function was also observed in PGAM2K176R/K176R P19 cells, a carcinoma cell line. These findings indicate that the PGAM2-K176R mutation impaired glycolysis and mitochondrial function. Gene ontology term analysis of RNA sequencing data further revealed that several downregulated genes in PGAM2K176R/K176R C2C12 cells were associated with muscle differentiation/development/contraction programs. Finally, PGAM2 with either of two naturally occurring missense mutations linked to GSDX, E89A (conversion of glutamic acid 89 to alanine) or R90W (conversion of arginine 90 to tryptophan), exhibited reduced Phosphoglycerate mutase 2 sumoylation. Thus, sumoylation is an important mechanism that mediates Phosphoglycerate mutase 2 activity and is potentially implicated in Phosphoglycerate mutase 2 mutation-linked disease in humans.
Transcriptional regulation of Satb1 in mouse trophoblast stem cells
Authors: Yu Wei , Chakravarthi V. Praveen , Borosha Shaon , Dilower Iman , Lee Eun Bee , Ratri Anamika , Starks Rebekah R. , Fields Patrick E. , Wolfe Michael W. , Faruque M. Omar , Tuteja Geetu , Rumi M. A. Karim
Published: December 2022

SATB homeobox proteins are important regulators of developmental gene expression. Among the stem cell lineages that emerge during early embryonic development, trophoblast stem (TS) cells exhibit robust SATB expression. Both SATB1 and SATB2 act to maintain the trophoblast stem-state. However, the molecular mechanisms that regulate TS-specific Satb expression are not yet known. We identified Satb1 variant 2 as the predominant transcript in trophoblasts. Histone marks, and RNA polymerase II occupancy in TS cells indicated an active state of the promoter. A novel cis-regulatory region with active histone marks was identified ∼21 kbp upstream of the variant 2 promoter. CRISPR/Cas9 mediated disruption of this sequence decreased Satb1 expression in TS cells and chromosome conformation capture analysis confirmed looping of this distant regulatory region into the proximal promoter. Scanning position weight matrices across the enhancer predicted two ELF5 binding sites in close proximity to SATB1 sites, which were confirmed by chromatin immunoprecipitation. Knockdown of ELF5 downregulated Satb1 expression in TS cells and overexpression of ELF5 increased the enhancer-reporter activity. Interestingly, ELF5 interacts with SATB1 in TS cells, and the enhancer activity was upregulated following SATB overexpression. Our findings indicate that trophoblast-specific Satb1 expression is regulated by long-range chromatin looping of an enhancer that interacts with ELF5 and SATB proteins.
Virus-like particle – mediated delivery of the RIG-I agonist M8 induces a type I interferon response and protects cells against viral infection
Authors: Palermo Enrico , Alexandridi Magdalini , Di Carlo Daniele , Muscolini Michela , Hiscott John
Published: December 2022

Virus-Like Particles (VLPs) are nanostructures that share conformation and self-assembly properties with viruses, but lack a viral genome and therefore the infectious capacity. In this study, we produced VLPs by co-expression of VSV glycoprotein (VSV-G) and HIV structural proteins (Gag, Pol) that incorporated a strong sequence-optimized 5’ppp-RNA RIG-I agonist, termed M8. Treatment of target cells with VLPs-M8 generated an antiviral state that conferred resistance against multiple viruses. Interestingly, treatment with VLPs-M8 also elicited a therapeutic effect by inhibiting ongoing viral replication in previously infected cells. Finally, the expression of SARS-CoV-2 Spike glycoprotein on the VLP surface retargeted VLPs to ACE2 expressing cells, thus selectively blocking viral infection in permissive cells. These results highlight the potential of VLPs-M8 as a therapeutic and prophylactic vaccine platform. Overall, these observations indicate that the modification of VLP surface glycoproteins and the incorporation of nucleic acids or therapeutic drugs, will permit modulation of particle tropism, direct specific innate and adaptive immune responses in target tissues, and boost immunogenicity while minimizing off-target effects.
Identification of PTPN22 as a potential genetic biomarker for abdominal aortic aneurysm
Authors: Ruan Peng , Gao Lei , Jiang Hui , Chu Tianshu , Ge Jianjun , Kong Xiang
Published: December 2022

Abdominal aortic aneurysm (AAA) is a severe life-threatening disease that is generally asymptomatic and is diagnosed at a very late stage. The genetic component underpinning AAA is considerable, with an estimated heritability of up to 70%. Therefore, identifying genetic biomarkers for AAA is valuable for predicting high-risk populations. We used integrative bioinformatics and cellular AAA model-based validation to reveal that the gene encoding protein tyrosine phosphatase non-receptor type 22 (PTPN22) may be a potentially useful diagnostic biomarker for AAA. Integrative bioinformatics analyses of clinical specimens showed that PTPN22 expression was consistently upregulated in aortic tissues and peripheral blood mononuclear cells (PBMCs) derived from patients with AAA. Moreover, transcriptomics data revealed that PTPN22 is a potential biomarker for AAA with limited diagnostic value in patients with thoracic aortic aneurysm/dissection. Single-cell RNA sequencing-based findings further highlight PTPN22 expression in aortic immune cells and vascular smooth muscle cells (VSMCs) is consistently upregulated in patients with AAA. A cellular AAA model was eventually employed to verify the increase in PTPN22 expression. Collectively, the results indicate that PTPN22 could be a potentially useful diagnostic biomarker for AAA.
Evolution of marine gravel dunes on the open shelf under multi-directional currents conditions
Authors: Ribó Marta , Watson Sally J. , Macdonald Helen , Strachan Lorna J.
Published: December 2022

On inner continental shelves, a variety of coarse grained bedforms, such as gravel dunes, are shaped by hydrodynamic and morphodynamic processes. The formation and evolution of bedforms reflect a balance between seabed and coastal morphology, sediment type and availability, and regional hydrodynamics. Yet, observing bedform evolution directly in the marine environment is rare, mostly due to the lack of repeat seafloor mapping surveys. In this study we use repeat bathymetry from 3 surveys over 4 years from the western Cook Strait/Te Moana-o-Raukawakawa region, New Zealand/Aotearoa. We integrate seabed morphology characterisation with sediment classification and regional hydrodynamic modelling, to investigate the evolution of gravel dunes under multi-directional current conditions. The repeat seafloor mapping reveals morphological changes to plan-view dune geometry and bifurcation of crestlines, with maximum observed vertical changes up to 3 m at water depths between 60 and 80 m. However, no dune migration was detected. Our hydrodynamic model shows that the most prominent morphological changes over the gravel dunes are spatially correlated with eddy formation, and high multi-directional near-bottom currents, reaching maximum speeds of ∼4 m s−1 and bottom stress of >25 N m−2 in each tidal cycle. We demonstrate that the average hydrodynamic conditions in this region are capable of mobilising coarse-grained sediment (i.e., sand to gravel), indicating that the observed morphological changes over multi-year time scales are a result of continuous remobilisation by currents, rather than extreme or storm events. Our findings demonstrate the highly dynamic nature of the seabed in Cook Strait, and the need for regular, repeat mapping surveys to ensure up-to-date seabed morphology information.
Health-promoting leadership in education: Relevance to teachers in township schools
Authors: Kwatubana Siphokazi
Published: December 2022

Teachers in township schools are vulnerable because of the low socio-economic conditions of the communities in which their schools are located. Such factors coupled with personal challenges can have a negative impact on their health. The aim of this research was to investigate the value of health-promoting leadership (HPL) in township schools. A qualitative approach was followed to gather information about the latter. Data were gathered by means of diary keeping, recording and individual interviews. Sixteen participants participated in the research and were engaged in the research for 4 months. A total of 32 interviews were conducted; each participant was interviewed twice. The findings revealed that HPL was perceived as relevant to the participating schools as it enhanced healthy working conditions, such as a health-promoting culture, health awareness and close working relationships (community). It is recommended that more attention be given to HPL, focusing on physical environments and safety and providing social support and health awareness programs to enhance teachers’ physical and mental health.
Infertile human endometrial organoid apical protein secretions are dysregulated and impair trophoblast progenitor cell adhesion
Authors: Zhou Wei , Barton Siena , Cui Jinwei , Santos Leilani L. , Yang Guannan , Stern Catharyn , Kieu Violet , Teh Wan Tinn , Ang Catarina , Lucky Tarana , Sgroi Joseph , Ye Louie , Dimitriadis Evdokia
Published: December 2022

Introduction

Embryo implantation failure leads to infertility. As an important approach to regulate implantation, endometrial epithelial cells produce and secrete factors apically into the uterine cavity in the receptive phase to prepare the initial blastocyst adhesion and implantation. Organoids were recently developed from human endometrial epithelium with similar apical-basal polarity compared to endometrial gland making it an ideal model to study endometrial epithelial secretions.

Methods

Endometrial organoids were established using endometrial biopsies from women with primary infertility and normal fertility. Fertile and infertile organoids were treated with hormones to model receptive phase of the endometrial epithelium and intra-organoid fluid (IOF) was collected to compare the apical protein secretion profile and function on trophoblast cell adhesion.

Results

Our data show that infertile organoids were dysregulated in their response to estrogen and progesterone treatment. Proteomic analysis of organoid apical secretions identified 150 dysregulated proteins between fertile and infertile groups (>1.5-fold change). Trophoblast progenitor spheroids (blastocyst surrogates) treated with infertile organoid apical secretions significantly compromised their adhesion to organoid epithelial cell monolayers compared to fertile group (P < 0.0001).

Discussion

This study revealed that endometrial organoid apical secretions alter trophoblast cell adhesiveness relative to fertility status of women. It paves the way to determine the molecular mechanisms by which endometrial epithelial apical released factors regulate blastocyst initial attachment and implantation.
Serum spexin differed in newly diagnosed type 2 diabetes patients according to body mass index and increased with the improvement of metabolic status
Authors: Gu Liping , Yan Shuai , Huang Yunhong , Yang Jiaying , Peng Yongde , Wang Yufan
Published: December 2022

Objective

The aim of this study was to explore serum spexin levels in newly diagnosed type 2 diabetes mellitus (T2DM) patients with different body mass indexes (BMIs) and to investigate the changes of spexin after improvement of metabolic indicators.

Methods

A total of 323 newly diagnosed T2DM patients from national Metabolic Management Center (MMC) in Shanghai General Hospital were recruited. T2DM patients were categorized into three groups: diabetes with obesity group (DM-OB group, BMI≥28 kg/m2, n=89), diabetes with overweight group (DM-OV group, 24≤BMI<28 kg/m2, n=161), and diabetes with normal weight group (DM-NW group, 18≤BMI<24 kg/m2, n=73). In addition, 41 volunteers with normal glucose tolerance (NGT) were used as controls. Spexin and metabolic parameters were compared at baseline, and changes after MMC follow-up in 100 DM patients were investigated.

Results

In the DM-OB group, the level of spexin was significantly lower than that in the DM-OV group and the DM-NW group (P < 0.01). Spexin was significantly negatively related to body mass index (BMI, β=-0.214, P<0.001), waist circumference (β=-0.249, P<0.001), visceral fat area (VFA, β=-0.214, P<0.001), and subcutaneous fat area (SFA, β=-0.265, P<0.001) after adjustment for age and sex. Among all the metabolic indicators, the decline in BMI in the DM-OB group was the most obvious among those in the three groups (-3.7 ± 0.8 kg/m2 vs. -0.9 ± 0.3 kg/m2 vs. 0.7 ± 0.6 kg/m2, P<0.01) after one year of MMC standardized management. The serum spexin level in the DM-OB group increased the most (1.00 ± 0.10 ng/mL vs. 0.49 ± 0.06 ng/mL in DM-OV group and 0.58 ± 0.09 ng/mL in DM-NW group, P < 0.001).

Conclusions

Serum spexin differed in newly diagnosed T2DM patients according to BMI and was lowest in the DM-OB group. With the improvement of metabolic indicators, especially the decline in BMI, serum spexin increased significantly after MMC management.
A cost-efficient DC active load laboratory solution
Authors: Santoro Danilo , Delmonte Nicola , Cova Paolo , Toscani Andrea
Published: December 2022

The increased use of DC renewable energy resources and DC storage systems, combined with the necessary reduction of energy waste, is boosting the development of DC smart grids. In this scenario, DC load emulation is of great importance. From the hardware point of view, DC buses stability of smart grids and the different DC/DC converter topologies must be tested. From the software point of view, smart grid strategies and job schedulers must be tested with different power absorption profiles. Moreover, DC load emulation can be useful for many other purposes, such as battery characterization, power supply testing, photovoltaic I-V curve measurements, etc. In this work, a cost-efficient DC Active Load (AL) solution is proposed. The principle of the circuit topology is a buck-boost-derived converter. This solution can be designed and tested considering the required voltage, current, and maximum input power. Both simulation and experimental results are shown on a 400 W size prototype. Thermal and electrical results validate the simulation model and the AL feasibility.
Nest-site choice by loggerhead sea turtles as a risk-management adaptation to offset hatching failure by unpredictable storms and predators
Authors: Cassill Deby L. , Watkins Alison
Published: December 2022

Introduction

Along the coasts of Florida, United States, the nesting season of the loggerhead sea turtle, Caretta caretta, overlaps with the hurricane season. Nesting loggerhead females do not extend parental protection beyond depositing eggs in sandy, excavated nests in locations that provide a viable range of temperatures, moisture, and respiratory gas exchange. Thereafter, a female’s clutches are subjected to the uncertainties of desiccation, predation, flooding, or beach erosion.

Methods

Here, we used data from a 1996-2004 study of 94 tagged loggerhead females nesting on a small barrier island off the Gulf Coast of south Florida, United States. We tested the hypothesis that the distribution of nest sites by loggerhead females was a randomized response to unpredictable hatching failure.

Results

We show that nest site choice accounted for 19.2% of variation in hatching success whereas breeding year and breeding month accounted for the remaining 81.8% of variation in hatching success. We show that the emergence site along the beach-length axis, nest site choice along the beach-width axis, and distances between nest locations did not fit a uniform-random distribution or a normal distribution. Instead, we show that loggerhead females employed a “Goldilocks” distribution in which nest sites were “neither too clustered nor too dispersed.” Moreover, loggerhead females selected nest sites with limited overlap with nest sites from previous breeding seasons.

Discussion

We propose that nest site choice by this population of loggerhead females constitutes a significant maternal risk-management adaptation that deserves thoughtful consideration as we continue to assess the impacts of climate change on the future of loggerhead sea turtles.
EPC infusion ameliorates acute graft-versus-host disease-related endothelial injury after allogeneic bone marrow transplantation
Authors: Wang Weiwei , Ye Yali , Du Yuwei , Xu Zhengqing , Yuan Ke , Wang Yizhou , Adzraku Seyram Yao , Li Yue , Xu Kailin , Qiao Jianlin , Ju Wen , Zeng Lingyu
Published: December 2022

Introduction

Graft-versus-host disease (GVHD) damages vascular endothelium. Endothelial progenitor cell (EPC) can differentiate to endothelial cell and promote angiogenesis, but its role in endothelial damage in GVHD is unclear.

Methods

In this study, we intend to assess whether EPC infusion promotes the repair of endothelial injury in GVHD mouse model. Male BALB/c mice were randomly divided into 5 groups: control group, total body irradiation group (TBI group), allogeneic bone marrow transplantation group (Allo-BMT group), acute graft versus host disease group (GVHD group), EPC infusion group (GVHD+EPC group) followed by analysis of mice survival, acute GVHD (aGVHD) score, T cell infiltration by immunofluorescence, as well as continuity of vascular endothelium in liver.

Results

Compared with Allo-BMT group, the clinical and pathological score of aGVHD mice were higher. On day 21 after transplantation, a large number of mononuclear cell infiltrations were seen in the target tissues of aGVHD mice and mice died within 30 days. In addition, aGVHD group also presented increased subendothelial infiltration of CD3+ T cells in the liver, decreased VE-cadherin expression and elevated major histocompatibility complex (MHC) II molecule expression in the endothelium. Moreover, expression of MHC-II molecule increased in endothelial cell after irradiation injury and LPS stimulation, indicating abnormally activated endothelial cell with antigen-presenting function. Interestingly, infusion of EPC reduced the clinical and pathological score of aGVHD, decreased infiltration of mononuclear cells, improved survival as well as upregulated VE-cadherin and downregulated MHC-II molecule.

Discussion

EPC infusion can mobilize to affected endothelium to decrease the infiltration of T cells and pathological endothelial activation contributing to ameliorating the damage of endothelium. EPC infusion combined with bone marrow transplantation might be a perspective strategy for the prevention and treatment of aGVHD.
mRNA vaccines for cancer immunotherapy
Authors: Vishweshwaraiah Yashavantha L. , Dokholyan Nikolay V.
Published: December 2022

Immunotherapy has emerged as a breakthrough strategy in cancer treatment. mRNA vaccines are an attractive and powerful immunotherapeutic platform against cancer because of their high potency, specificity, versatility, rapid and large-scale development capability, low-cost manufacturing potential, and safety. Recent technological advances in mRNA vaccine design and delivery have accelerated mRNA cancer vaccines’ development and clinical application. In this review, we present various cancer vaccine platforms with a focus on nucleic acid vaccines. We discuss rational design and optimization strategies for mRNA cancer vaccine development. We highlight the platforms available for delivery of the mRNA vaccines with a focus on lipid nanoparticles (LNPs) based delivery systems. Finally, we discuss the limitations of mRNA cancer vaccines and future challenges.
Altered serum human cytomegalovirus microRNA levels are common and closely associated with the inflammatory status in patients with fever
Authors: Wang Cheng , Zhu Yunhua , Chen Penglu , Wang Chen , Zhou Wanqing , Zhang Cuiping , Wang Jing , Chen Xi , Ding Meng , Zhang Chunni , Wang Jun-Jun , Zhang Chen-Yu
Published: December 2022

Background

Fever has a complicated etiology, and diagnosing its causative factor is clinically challenging. Human cytomegalovirus (HCMV) infection causes various diseases. However, the clinical relevance, prevalence, and significance of HCMV microRNAs (miRNA) in association with fever remain unclear. In the present study, we analyzed the HCMV miRNA expression pattern in the serum of patients with fever and evaluate its clinical associations with occult HCMV infection status in immune disorders.

Methods

We included serum samples from 138 patients with fever and 151 age-gender-matched controls in this study. First, the serum levels of 24 HCMV miRNAs were determined using a hydrolysis probe-based stem-loop quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay in the training set. The markedly altered miRNAs were verified in the validation and testing sets. The serum HCMV IgG/IgM and DNA titers in the testing cohort were also assessed using enzyme-linked immunosorbent assay (ELISA) and RT-qPCR, respectively.

Results

The majority of HCMV miRNAs were markedly upregulated in the serum of fever patients. We selected the five most significantly altered HCMV miRNAs: hcmv-miR-US4-3p, hcmv-miR-US29-3p, hcmv-miR-US5-2-3p, hcmv-miR-UL112-3p, and hcmv-miR-US33-3p for validation. These miRNAs were also significantly elevated in the serum of fever patients in the validation and testing sets compared with the controls. Logistic regression analysis revealed that the five miRNAs were novel potential risk factors for fever. Notably, the serum levels of four of the five confirmed HCMV miRNAs were significantly associated with blood C-reaction protein concentrations. Moreover, the five HCMV miRNA levels were closely correlated with the HCMV DNA titers in the testing cohort.

Conclusion

HCMV infection and activation are common in fever patients and could be novel risk factors for fever. These differentially expressed HCMV miRNAs could enable HCMV activation status monitoring in immune disorders.