bluuebunny/crossref_metadata_2025 huggingface dataset:
Shear Stress‐Induced Total Blood Trauma in Multiple Species
Authors: Chan Chris H. H. , Pieper Ina Laura , Robinson Christian R. , Friedmann Yasmin , Kanamarlapudi Venkateswarlu , Thornton Catherine A.
Published: July 2017

The common complications in heart failure patients with implanted ventricular assist devices (VADs) include hemolysis, thrombosis, and bleeding. These are linked to shear stress‐induced trauma to erythrocytes, platelets, and von Willebrand factor (vWF). Novel device designs are being developed to reduce the blood trauma, which will need to undergo in vitro and in vivo preclinical testing in large animal models such as cattle, sheep, and pig. To fully understand the impact of device design and enable translation of preclinical results, it is important to identify any potential species‐specific differences in the VAD‐associated common complications. Therefore, the purpose of this study was to evaluate the effects of shear stress on cells and proteins in bovine, ovine, and porcine blood compared to human. Blood from different species was subjected to various shear rates (0–8000/s) using a rheometer. It was then analyzed for complete blood counts, hemolysis by the Harboe assay, platelet activation by flow cytometry, vWF structure by immunoblotting, and function by collagen binding activity ELISA (vWF : CBA). Overall, increasing shear rate caused increased total blood trauma in all tested species. This analysis revealed species‐specific differences in shear‐induced hemolysis, platelet activation, and vWF structure and function. Compared to human blood, porcine blood was the most resilient and showed less hemolysis, similar blood counts, but less platelet activation and less vWF damage in response to shear. Compared to human blood, sheared bovine blood showed less hemolysis, similar blood cell counts, greater platelet activation, and similar degradation of vWF structure, but less impact on its activity in response to shear. The shear‐induced effect on ovine blood depended on whether the blood was collected via gravity at the abattoir or by venepuncture from live sheep. Overall, ovine abattoir blood was the least resilient in response to shear and bovine blood was the most similar to human blood. These results lay the foundations for developing blood trauma evaluation standards to enable the extrapolation of in vitro and in vivo animal data to predict safety and biocompatibility of blood‐handling medical devices in humans. We advise using ovine venepuncture blood instead of ovine abattoir blood due to the greater overall damage in the latter. We propose using bovine blood for total blood damage in vitro device evaluation but multiple species could be used to create a full understanding of the complication risk profile of new devices. Further, this study highlights that choice of antibody clone for evaluating platelet activation in bovine blood can influence the interpretation of results from different studies.
Privilege and Marginality: How Group Identification and Personality Predict Right‐ and Left‐Wing Political Activism
Authors: Blankenship Benjamin T. , Frederick Jennifer K. , Savaş Özge , Stewart Abigail J. , Montgomery Samantha
Published: July 2017

In two studies, we examine how different processes might underlie the political mobilization of individuals with marginalized versus privileged identities for left‐wing activism (LWA) versus right‐wing activism (RWA). In the first study, with a sample of 244 midlife women, we tested the hypotheses that endorsement of system justification beliefs and social identities were direct predictors of political activism, and that system justification beliefs moderated the mobilization of social identities for activism on both the left and the right. We found that system justification predicted RWA only among those who felt close to privileged groups; the parallel reverse effect did not hold for LWA, though rejection of system‐justifying beliefs was an important direct predictor. In Study 2, we replicated many of these findings with a sample of 113 college students. In addition, we tested and confirmed the hypothesis that LWA is predicted by openness to experience and is unrelated to RWA, but not that openness plays a stronger role among those with marginalized identities. These two studies together support our overall hypothesis that different personality processes are involved with political mobilization of privileged and marginalized individuals on the right and the left.
Contra‐Associational Strategy in a Hybrid Regime: Ecuador, 2007–2015
Authors: CONAGHAN CATHERINE M.
Published: July 2017

Over the last decade, political scientists have identified Ecuador as one of Latin America's hybrid regimes. This article examines how President Rafael Correa combined legal reforms, bureaucratic controls and other policies in a contra‐associational strategy aimed at extending executive control over civil society. While the strategy significantly altered the operational environment for civil society groups, it did not completely strip them of their capacity to oppose the regime. Ecuador's experience underscores the ambiguity at play in hybrids; in pursuit of regime legitimation, regimes must cede some space to opponents while simultaneously sabotaging civil liberties.
Trimethylamine N‐oxide: breathe new life
Authors: Subramaniam Saravanan , Fletcher Craig
Published: July 2017

Association between elevated levels of systemic trimethylamine N‐oxide (TMAO) and increased risk for adverse cardiovascular events have been proposed in recent years. Increasing experimental and clinical evidence in the last decade has implicated TMAO as an important contributor to the pathogenesis of cardiovascular diseases. TMAO, the oxygenated product of trimethylamine (TMA), belongs to the class of amine oxides. Most of the TMA derived from the metabolism of choline and L‐carnitine by gut bacteria is absorbed into the bloodstream and gets rapidly oxidized to TMAO by the hepatic enzyme, flavin‐containing monooxgenase‐3. Here, we discussed the biosynthesis of TMAO and clinical studies that have assessed TMAO as a biomarker for various cardiovascular and other diseases such as kidney failure, thrombosis, atherosclerosis, obesity, diabetes and cancer. We also summarized the interaction of TMAO with synthetic and traditional molecules that together affect circulating TMAO levels.

Linked Articles

This article is part of a themed section on Spotlight on Small Molecules in Cardiovascular Diseases. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v175.8/issuetoc
Association between coffee consumption and all‐sites cancer incidence and mortality
Authors: Sado Junya , Kitamura Tetsuhisa , Kitamura Yuri , Sobue Tomotaka , Nishino Yoshikazu , Tanaka Hideo , Nakayama Tomio , Tsuji Ichiro , Ito Hidemi , Suzuki Takaichiro , Katanoda Kota , Tominaga Suketami ,   the Three‐Prefecture Cohort Study Group
Published: July 2017

The preventive effect of coffee on cancer at different sites has been reported, but the effect on all‐sites cancer incidence has not been extensively investigated. We evaluated the association between frequency of coffee consumption and risk of all‐sites cancer incidence and mortality among 39 685 men and 43 124 women (age 40–79 years, at baseline), in the Three‐Prefecture Cohort Study. The association between frequency of coffee consumption and risk of all‐sites cancer incidence and mortality was assessed by a Cox proportional hazards regression model, adjusted for potential confounders. During 411 341 person‐years among men and 472 433 person‐years among women, a total of 4244 men and 2601 women developed cancer at different sites and a total of 3021 men and 1635 women died of cancer at different sites. We showed an inverse association between frequency of coffee consumption and all‐sites cancer incidence in both men and women. Comparing participants who consumed coffee with those who never drank coffee, the adjusted hazard ratios (95% confidential interval) for all‐sites cancer incidence was 0.74 (0.62–0.88) for coffee consumption of ≥5 cups/day in men (P for trend < 0.001) and 0.76 (0.58–1.02) in women (P for trend = 0.020). Coffee consumption frequency was inversely associated with mortality from all‐sites cancer. In this population, increasing coffee consumption resulted in a decreased risk of all‐sites cancer incidence and mortality.
Lack of interleukin‐6 in the tumor microenvironment augments type‐1 immunity and increases the efficacy of cancer immunotherapy
Authors: Ohno Yosuke , Toyoshima Yujiro , Yurino Hideaki , Monma Norikazu , Xiang Huihui , Sumida Kentaro , Kaneumi Shun , Terada Satoshi , Hashimoto Shinichi , Ikeo Kazuho , Homma Shigenori , Kawamura Hideki , Takahashi Norihiko , Taketomi Akinobu , Kitamura Hidemitsu
Published: July 2017

Conquering immunosuppression in tumor microenvironments is crucial for effective cancer immunotherapy. It is well known that interleukin (IL)‐6, a pleiotropic cytokine, is produced in the tumor‐bearing state. In the present study, we investigated the precise effects of IL‐6 on antitumor immunity and the subsequent tumorigenesis in tumor‐bearing hosts. CT26 cells, a murine colon cancer cell line, were intradermally injected into wild‐type and IL‐6‐deficient mice. As a result, we found that tumor growth was decreased significantly in IL‐6‐deficient mice compared with wild‐type mice and the reduction was abrogated by depletion of CD8+ T cells. We further evaluated the immune status of tumor microenvironments and confirmed that mature dendritic cells, helper T cells and cytotoxic T cells were highly accumulated in tumor sites under the IL‐6‐deficient condition. In addition, higher numbers of interferon (IFN)‐γ‐producing T cells were present in the tumor tissues of IL‐6‐deficient mice compared with wild‐type mice. Surface expression levels of programmed death‐ligand 1 (PD‐L1) and MHC class I on CT26 cells were enhanced under the IL‐6‐deficient condition in vivo and by IFN‐γ stimulation in vitro. Finally, we confirmed that in vivo injection of an anti‐PD‐L1 antibody or a Toll‐like receptor 3 ligand, polyinosinic‐polycytidylic acid, effectively inhibited tumorigenesis under the IL‐6‐deficient condition. Based on these findings, we speculate that a lack of IL‐6 produced in tumor‐bearing host augments induction of antitumor effector T cells and inhibits tumorigenesis in vivo, suggesting that IL‐6 signaling may be a promising target for the development of effective cancer immunotherapies.
Regulation of spindle and kinetochore‐associated protein 1 by antitumor miR‐10a‐5p in renal cell carcinoma
Authors: Arai Takayuki , Okato Atsushi , Kojima Satoko , Idichi Tetsuya , Koshizuka Keiichi , Kurozumi Akira , Kato Mayuko , Yamazaki Kazuto , Ishida Yasuo , Naya Yukio , Ichikawa Tomohiko , Seki Naohiko
Published: July 2017

Analysis of our original microRNA (miRNA) expression signature of patients with advanced renal cell carcinoma (RCC) showed that microRNA‐10a‐5p (miR‐10a‐5p) was significantly downregulated in RCC specimens. The aims of the present study were to investigate the antitumor roles of miR‐10a‐5p and the novel cancer networks regulated by this miRNA in RCC cells. Downregulation of miR‐10a‐5p was confirmed in RCC tissues and RCC tissues from patients treated with tyrosine kinase inhibitors (TKI). Ectopic expression of miR‐10a‐5p in RCC cell lines (786‐O and A498 cells) inhibited cancer cell migration and invasion. Spindle and kinetochore‐associated protein 1 (SKA1) was identified as an antitumor miR‐10a‐5p target by genome‐based approaches, and direct regulation was validated by luciferase reporter assays. Knockdown of SKA1 inhibited cancer cell migration and invasion in RCC cells. Overexpression of SKA1 was observed in RCC tissues and TKI‐treated RCC tissues. Moreover, analysis of The Cancer Genome Atlas database demonstrated that low expression of miR‐10a‐5p and high expression of SKA1 were significantly associated with overall survival in patients with RCC. These findings showed that downregulation of miR‐10a‐5p and overexpression of the SKA1 axis were highly involved in RCC pathogenesis and resistance to TKI treatment in RCC.
Measuring socioeconomic inequalities in the use of dental care services among older adults in Brazil
Authors: Bof de Andrade Fabíola , Drumond Andrade Flávia Cristina , Noronha Kenya
Published: July 2017

Objectives

The primary objectives are to assess socioeconomic inequality in the use of dental care among older Brazilian adults and to analyse the extent to which certain determinants contribute to that inequality.

Methods

A cross‐sectional study using data from the National Oral Health Survey conducted in 2010. All individuals answered a structured questionnaire containing questions on their use of dental care and socioeconomic conditions and underwent a clinical oral examination by a dentist. Concentration indices were decomposed to determine the contribution of socioeconomic factors to inequalities.

Results

Being in the fifth wealth, quintile was associated with higher odds of having recently visited a dentist (reference: 1st quintile, odds‐ratio (OR) 2.26, 95% confidence interval (CI) 1.51‐3.38). In addition, being in the top two quintiles of wealth was negatively associated with the use of public dental services. Having eight or more years of schooling was associated with higher odds of both having a recent dental visit and receiving preventive care (relative to having 0‐3 years of education), and negatively associated with using public dental services. Results indicate pro‐rich inequalities in recent dental visits and preventive dental care. Further, there was a pro‐poor inequality in the use of public dental care services.

Conclusions

The recent use of dental care and the use of preventive care are disproportionately concentrated among wealthier older adults, whereas the use of public services is more common among poorer individuals. Wealth inequalities in dental care use were mainly explained by socioeconomic factors, such as wealth and education, rather than oral health factors, such as needing treatment or a dental prosthesis.
Input and Age‐Dependent Variation in Second Language Learning: A Connectionist Account
Authors: Janciauskas Marius , Chang Franklin
Published: July 2017

Language learning requires linguistic input, but several studies have found that knowledge of second language (L2) rules does not seem to improve with more language exposure (e.g., Johnson & Newport, 1989). One reason for this is that previous studies did not factor out variation due to the different rules tested. To examine this issue, we reanalyzed grammaticality judgment scores in Flege, Yeni‐Komshian, and Liu's (1999) study of L2 learners using rule‐related predictors and found that, in addition to the overall drop in performance due to a sensitive period, L2 knowledge increased with years of input. Knowledge of different grammar rules was negatively associated with input frequency of those rules. To better understand these effects, we modeled the results using a connectionist model that was trained using Korean as a first language (L1) and then English as an L2. To explain the sensitive period in L2 learning, the model's learning rate was reduced in an age‐related manner. By assigning different learning rates for syntax and lexical learning, we were able to model the difference between early and late L2 learners in input sensitivity. The model's learning mechanism allowed transfer between the L1 and L2, and this helped to explain the differences between different rules in the grammaticality judgment task. This work demonstrates that an L1 model of learning and processing can be adapted to provide an explicit account of how the input and the sensitive period interact in L2 learning.
Correlation‐based classifier combination in the field of pattern recognition
Authors: Singh Pawan Kumar , Sarkar Ram , Nasipuri Mita
Published: July 2017

Classifier combination methods have proved to be an effective tool to increase the performance of classification techniques that can be used in any pattern recognition applications. Despite a significant number of publications describing successful classifier combination implementations, the theoretical basis is still not matured enough and achieved improvements are inconsistent. In this paper, we propose a novel statistical validation technique known as correlation‐based classifier combination technique for combining classifier in any pattern recognition problem. This validation has significant influence on the performance of combinations, and their utilization is necessary for complete theoretical understanding of combination algorithms. The analysis presented is statistical in nature but promises to lead to a class of algorithms for rank‐based decision combination. The potentials of the theoretical and practical issues in implementation are illustrated by applying it on 2 standard datasets in pattern recognition domain, namely, handwritten digit recognition and letter image recognition datasets taken from UCI Machine Learning Database Repository (http://www.ics.uci.edu/_mlearn). An empirical evaluation using 8 well‐known distinct classifiers confirms the validity of our approach compared to some other combinations of multiple classifiers algorithms. Finally, we also suggest a methodology for determining the best mix of individual classifiers.
863. BERBERIS ZHAOTONGENSIS
Authors: Harber Julian
Published: July 2017

A new species of Berberis, in sect. Wallichianae, Berberis zhaotongensis Harber is described and illustrated.
866. PASSIFLORA MACROPHYLLA
Authors: Vanderplank John , Edwards Sara , Hilgenhof Rebecca , Rodriguez Carlos Magdalena
Published: July 2017

Passiflora macrophylla Spruce ex Mast., a small weak tree from Ecuador and Colombia, is described and illustrated. Its history and cultivation are discussed.
THE ENIGMA OF PLEIONE ‘FRANK KINGDON WARD’
Authors: Cribb Phillip , Butterfield Ian
Published: July 2017

Pleione ‘Frank Kingdon Ward’ first appeared in collections in the 1950s and has persisted. It has been considered to be a form of the spring flowered Pleione humilis but cytological examination shows it to be a triploid hybrid. Its identity is examined here and a formal hybrid name is proposed for it.
Evaluation of a professional development training programme for mental health clinicians specializing in early psychosis
Authors: Macneil Craig , Foster Frances , Nicoll Amanda , Osman Helen , Monfries Richard , Cotton Sue
Published: July 2017

The Early Psychosis Prevention and Intervention Centre Statewide Services (ESW) team provides training for multidisciplinary clinicians that specialise in early psychosis across the State of Victoria, Australia. The aim of this paper is to describe the 4‐phase approach utilised by ESW to prepare for and deliver workshops, to report on participants’ ratings of the ESW workshops, and to make recommendations for other trainers of early psychosis clinicians. Between March 2009 and September 2014, ESW provided 85 training workshops that had a strong focus on evidence‐based approaches and international guidelines, and utilized clinical examples of early psychosis interventions. At the conclusion of each workshop, participants were asked to complete a questionnaire that comprised qualitative and quantitative elements. These assessed perceived trainer knowledge, learning, interactivity and specific topic feedback. The focus of this paper will be on describing the quantitative data resulting from these questionnaires. A total of 1708 clinicians provided feedback on the 85 workshops. There was a high level of compliance, with 83.0% of workshop participants completing the questionnaires. Feedback was positive across all areas, with the 2 areas that were most highly endorsed being that presenters “appeared to know their subject matter well” (endorsed by 98.4% of participants) and that “topics were explained well” (endorsed by 96.8% of participants). Training for early psychosis clinicians that focusses on core clinical topics, is well planned, incorporates feedback from previous training, and is based on adult learning principles, is likely to be effective and well received by early psychosis clinicians.
Multivariate mapping of soil with structural equation modelling
Authors: Angelini M. E. , Heuvelink G. B. M. , Kempen B.
Published: July 2017

In a previous study we introduced structural equation modelling (SEM) for digital soil mapping in the Argentine Pampas. An attractive property of SEM is that it incorporates pedological knowledge explicitly through a mathematical implementation of a conceptual model. Many soil processes operate within the soil profile; therefore, SEM might be suitable for simultaneous prediction of soil properties for multiple soil layers. In this way, relations between soil properties in different horizons can be included that might result in more consistent predictions. The objectives of this study were therefore to apply SEM to multi‐layer and multivariate soil mapping, and to test SEM functionality for suggestions to improve the modelling. We applied SEM to model and predict the lateral and vertical distribution of the cation exchange capacity (CEC), organic carbon (OC) and clay content of three major soil horizons, A, B and C, for a 23 000‐km2 region in the Argentine Pampas. We developed a conceptual model based on pedological hypotheses. Next, we derived a mathematical model and calibrated it with environmental covariates and soil data from 320 soil profiles. Cross‐validation of predicted soil properties showed that SEM explained only marginally more of the variance than a linear regression model. However, assessment of the covariation showed that SEM reproduces the covariance between variables much more accurately than linear regression. We concluded that SEM can be used to predict several soil properties in multiple layers by considering the interrelations between soil properties and layers.

Highlights

We tested structural equation modelling (SEM) for multi‐layer and multivariate soil mapping.

SEM models soil property covariation better than multiple linear regression.

The SEM re‐specification step improves prediction accuracy.

SEM supports learning about soil processes from data.
The rove beetle Drusilla sparsa (Coleoptera: Staphylinidae) is a myrmecophilous species associated with a myrmicine ant, Crematogaster osakensis (Hymenoptera: Formicidae)
Authors: Ikeshita Yohei , Taniguchi Kentaro , Kitagawa Yuji , Maruyama Munetoshi , Ito Fuminori
Published: July 2017

The rove beetle genus Drusilla includes some myrmecophilous species. The Japanese species Drusilla sparsa (Sharp, 1874) has been regarded as a non‐myrmecophilous beetle. In Kagawa Prefecture, Shikoku Island, western Japan, however, we often observed that D. sparsa adults were walking in the vicinity of foraging workers of the myrmicine ant Crematogaster osakensis Forel, 1990. The body color of the beetle is similar to C. osakensis as in other myrmecophilous beetles found near the trails of the host ants. To examine whether D. sparsa is myrmecophilous, we investigated the distribution of D. sparsa and C. osakensis in the field, as well as their behavior including prey preference of the beetle in the laboratory. Drusilla sparsa beetles were collected only in sites where C. osakensis ants occurred. When the beetles encountered the ant workers, they bent the abdominal tip toward the ants. The ants licked the abdominal tip, and then the beetles usually walked away. Such behavioral reaction of the ants was not observed when the beetles encountered workers of the formicine ant Nylanderia flavipes (Smith, 1874) that continuously attacked the beetles. Drusilla sparsa preferred to feed on dead workers of C. osakensis even when other ants were available as food, indicating that D. sparsa is a myrmecophilous species associated with C. osakensis. Crematogaster osakensis was frequently found in the stomach in the ant predator, the Japanese treefrog Hyla japonica Günther, 1859. Thus, the significance of body color similarity between the host ants and beetles is not a case of Batesian mimicry.
Voxel‐based magnetic resonance image postprocessing in epilepsy
Authors: Martin Pascal , Winston Gavin P. , Bartlett Philippa , de Tisi Jane , Duncan John S. , Focke Niels K.
Published: July 2017

Objective

Although the general utility of voxel‐based processing of structural magnetic resonance imaging (MRI) data for detecting occult lesions in focal epilepsy is established, many differences exist among studies, and it is unclear which processing method is preferable. The aim of this study was to compare the ability of commonly used methods to detect epileptogenic lesions in magnetic resonance MRI‐positive and MRI‐negative patients, and to estimate their diagnostic yield.

Methods

We identified 144 presurgical focal epilepsy patients, 15 of whom had a histopathologically proven and MRI‐visible focal cortical dysplasia; 129 patients were MRI negative with a clinical hypothesis of seizure origin, 27 of whom had resections. We applied four types of voxel‐based morphometry (VBM), three based on T1 images (gray matter volume, gray matter concentration, junction map [JM]) and one based on normalized fluid‐attenuated inversion recovery (nFSI). Specificity was derived from analysis of 50 healthy controls.

Results

The four maps had different sensitivity and specificity profiles. All maps showed detection rates for focal cortical dysplasia patients (MRI positive and negative) of >30% at a strict threshold of p < 0.05 (family‐wise error) and >60% with a liberal threshold of p < 0.0001 (uncorrected), except for gray matter volume (14% and 27% detection rate). All maps except nFSI showed poor specificity, with high rates of false‐positive findings in controls. In the MRI‐negative patients, absolute detection rates were lower. A concordant nFSI finding had a significant positive odds ratio of 7.33 for a favorable postsurgical outcome in the MRI‐negative group. Spatial colocalization of JM and nFSI was rare, yet showed good specificity throughout the thresholds.

Significance

All VBM variants had specific diagnostic properties that need to be considered for an adequate interpretation of the results. Overall, structural postprocessing can be a useful tool in presurgical diagnostics, but the low specificity of some maps has to be taken into consideration.
Male territorial aggression does not drive conformity to local vocal culture in a passerine bird
Authors: Parra Marcelina , Dalisio Anthony C. , Jensen William E. , Parker Timothy H.
Published: July 2017

In many songbird species, young individuals learn songs from neighbors and then settle nearby, thus creating neighborhoods of conformity to local vocal culture. In some species, individuals appear to postpone song learning until after dispersal, possibly to facilitate conformity to local dialects. Despite decades of study, we still lack a consensus regarding the selective pressures driving this delayed song learning. Two common hypothetical benefits to conformity, and thus delayed song learning, are rooted in territorial interactions; individuals preferentially produce local song either to avoid detection as new arrivals (deceptive mimicry) or to be more effectively recognized as conspecific territory holders. The dickcissel (Spiza americana) is an ideal species in which to study these hypotheses. Males of this species appear to delay song learning until they arrive at their first adult territory, each individual sings a single song type, and conformity to the local song culture is high. Using playback, we contradicted both of the territorial hypotheses described above; male dickcissels did not respond differentially to local vs foreign song playback treatment. We are confident in this lack of difference because dickcissels clearly responded less strongly to a third treatment, neighbor song, than to the other two treatments, demonstrating sufficient power in our experimental design (and providing the first evidence of the dear‐enemy effect in dickcissels). Our results raise the question of why dickcissels respond equally aggressively to both local and foreign songs when other bird species often show reduced aggression toward foreign song. If reduced aggression to foreign song is not ubiquitous in species that achieve conformity through delayed learning, then selection from aggressive territorial interaction seems unlikely to be a general explanation for such delayed learning. Reduced aggression in response to foreign songs in other species may be due to reduced exposure to the stimulus of foreign song or to different cost‐benefit trade‐offs when responding to songs that deviate from the local average.
What is driving male mate preference evolution in Jamaican field crickets?
Authors: Bertram Susan M. , Harrison Sarah J. , Ferguson Genevieve L. , Thomson Ian R. , Loranger Michelle J. , Reifer Mykell L. , Corlett Deborah H. , Gowaty Patricia Adair
Published: July 2017

Male mating preferences are often a neglected aspect of studies on sexual selection. Male mating preferences may evolve if they provide males with direct‐fitness benefits such as increased opportunity to fertilize more eggs or indirect‐fitness benefits such as enhanced offspring survival. We tested these ideas using Jamaican field crickets, Gryllus assimilis, previously shown to exhibit male mating preferences. We randomly mated males to either their preferred or non‐preferred potential mates and then asked whether mating treatment influenced egg oviposition or offspring viability. Preferred females were not significantly more fecund and did not produce more viable eggs or offspring than non‐preferred females. Male mate preferences were therefore inconsistent with both the direct‐ and indirect‐fitness benefits hypotheses under the conditions of our experiment. Our null results leave us with an open question about what is driving the evolution of mating preferences in male crickets. Future research should explore the whether the offspring of preferred females are more attractive, have stronger immune systems, and/or experience higher adult longevity.
Personality predicts ectoparasite abundance in an asocial sciurid
Authors: Bohn Shelby J. , Webber Quinn M. R. , Florko Katie R. N. , Paslawski Katlyn R. , Peterson Amelia M. , Piche Julia E. , Menzies Allyson K. , Willis Craig K. R.
Published: July 2017

Parasitism is a consequence of complex interactions between host, parasite, and their shared environment, and host behavior can influence parasite risk. Animal personality (i.e., consistent behavioral differences that are repeatable across time and context) can influence parasitism with more explorative individuals typically hosting greater parasite loads. Host “sociality” is known to impact parasite risk with more social individuals typically at higher risk of acquiring or transmitting parasites, but other behaviors could also be important. We quantified personality in least chipmunks (Tamias minimus), including repeatability of behavioral traits, and determined whether these personality traits affected ectoparasite prevalence and abundance. We measured personality using standardized hole‐board tests and quantified ectoparasitism of 39 least chipmunks over 2 years at a site in southeastern Manitoba, Canada. We found that activity and exploration were repeatable within the context of the hole‐board test for least chipmunks, which suggests that these traits reflect personality. More exploratory individuals hosted a greater abundance of ectoparasites compared to less exploratory individuals. Our results are consistent with past studies implicating personality as a factor in host–parasite dynamics and suggest that exploration may be an important behavioral correlate of parasite acquisition.
Amphibian species traits, evolutionary history and environment predict Batrachochytrium dendrobatidis infection patterns, but not extinction risk
Authors: Greenberg Dan A. , Palen Wendy J. , Mooers Arne Ø.
Published: July 2017

The fungal pathogen Batrachochytrium dendrobatidis (B. dendrobatidis) has emerged as a major agent of amphibian extinction, requiring conservation intervention for many susceptible species. Identifying susceptible species is challenging, but many aspects of species biology are predicted to influence the evolution of host resistance, tolerance, or avoidance strategies towards disease. In turn, we may expect species exhibiting these distinct strategies to differ in their ability to survive epizootic disease outbreaks. Here, we test for phylogenetic and trait‐based patterns of B. dendrobatidis infection risk and infection intensity among 302 amphibian species by compiling a global data set of B. dendrobatidis infection surveys across 95 sites. We then use best‐fit models that associate traits, taxonomy and environment with B. dendrobatidis infection risk and intensity to predict host disease mitigation strategies (tolerance, resistance, avoidance) for 122 Neotropical amphibian species that experienced epizootic B. dendrobatidis outbreaks, and noted species persistence or extinction from these events. Aspects of amphibian species life history, habitat use and climatic niche were consistently linked to variation in B. dendrobatidis infection patterns across sites around the world. However, predicted B. dendrobatidis infection risk and intensity based on site environment and species traits did not reveal a consistent pattern between the predicted host disease mitigation strategy and extinction outcome. This suggests that either tolerant or resistant species may have no advantage in ameliorating disease during epizootic events, or that other factors drive the persistence of amphibian populations during chytridiomycosis outbreaks. These results suggest that using a trait‐based approach may allow us to identify species with resistance or tolerance to endemic B. dendrobatidis infections, but that this approach may be insufficient to ultimately identify species at risk of extinction from epizootics.
Application of continuum damage mechanics to vibration fatigue life prediction
Authors: Li Yi , Sun Bing , Fang Jie , Liu Xue‐Feng , Liang Tong , Cai Guo‐Biao
Published: July 2017

It is pivotal to predict the multiaxial vibration fatigue life during mechanical structural dynamics design. An algorithm of the finite element method implementation for multiaxial high cycle fatigue life evaluation is proposed, on the basis of elastic evolution model of continuum damage mechanics. By considering structural dynamic characteristics, namely, resonant frequencies and mode shapes, this algorithm includes a modal analysis and harmonic analysis, which makes this different from existing fatigue life prediction methods. A 10% decrease in the resonant frequency is regarded as the failure criterion. A critical damage value was obtained, which indicates mesocrack initiation fulfilment. To validate the effectiveness of the algorithm, auto‐phase sine resonance track‐and‐dwell experiments were conducted on notched cantilever beams made of Ti‐6Al‐4V alloy. The life predictions are conservative and in good agreements with the experimental results, which are mainly distributed within a scatter band of 2. This investigation could provide technical support for structural dynamics design and the analysis of reusable spacecraft.
A weight function method for mixed modes hole‐edge cracks
Authors: Xu W. , Wu X.R. , Yu Y. , Li Z.H.
Published: July 2017

A weight function approach is proposed to calculate the stress intensity factor and crack opening displacement for cracks emanating from a circular hole in an infinite sheet subjected to mixed modes load. The weight function for a pure mode II hole‐edge crack is given in this paper. The stress intensity factors for a mixed modes hole‐edge crack are obtained by using the present mode II weight function and existing mode I Green (weight) function for a hole‐edge crack. Without complex derivation, the weight functions for a single hole‐edge crack and a centre crack in infinite sheets are used to study 2 unequal‐length hole‐edge cracks. The stress intensity factor and crack opening displacement obtained from the present weight function method are compared well with available results from literature and finite element analysis. Compared with the alternative methods, the present weight function approach is simple, accurate, efficient, and versatile in calculating the stress intensity factor and crack opening displacement.
Spatial evaluation of Indonesia's 2015 fire‐affected area and estimated carbon emissions using Sentinel‐1
Authors: Lohberger Sandra , Stängel Matthias , Atwood Elizabeth C. , Siegert Florian
Published: July 2017

Fires raged once again across Indonesia in the latter half of 2015, creating a state of emergency due to poisonous smoke and haze across Southeast Asia as well as incurring great financial costs to the government. A strong El Niño‐Southern Oscillation (ENSO) led to drought in many parts of Indonesia, resulting in elevated fire occurrence comparable with the previous catastrophic event in 1997/1998. Synthetic Aperture Radar (SAR) data promise to provide improved detection of land use and land cover changes in the tropics as compared to methodologies dependent upon cloud‐ and haze‐free images. This study presents the first spatially explicit estimates of burned area across Sumatra, Kalimantan, and West Papua based on high‐resolution Sentinel‐1A SAR imagery. Here, we show that 4,604,569 hectares (ha) were burned during the 2015 fire season (overall accuracy 84%), and compare this with other existing operational burned area products (MCD64, GFED4.0, GFED4.1s). Intersection of burned area with fine‐scale land cover and peat layer maps indicates that 0.89 gigatons carbon dioxide equivalents (Gt CO2e) were released through the fire event. This result is compared to other estimates based on nonspatially explicit thermal anomaly measurements or atmospheric monitoring. Using freely available SAR C‐band data from the Sentinel mission, we argue that the presented methodology is able to quickly and precisely detect burned areas, supporting improvement in fire control management as well as enhancing accuracy of emissions estimation.
Predicted factors for older Taiwanese to be healthy octogenarians: Results of an 18‐year national cohort study
Authors: Hsu Wei‐Chung , Tsai Alan C. , Chen Yu‐Chia , Wang Jiun‐Yi
Published: July 2017

Aim

To identify factors that predict the 62–69 years old Taiwanese to be healthy octogenarians.

Methods

We analyzed the 1989 (baseline), and 2003 and 2007 (end‐point) datasets of the Taiwan Longitudinal Survey on Aging, a national cohort study. A total of 1977 participants aged 62–69 years at baseline were tracked for 14–18 years. The outcome measure was “being healthy octogenarians”, defined as participants who were aged ≥80 years, free from activities of daily living dependency, depressive symptoms or cognitive impairment, and able to provide social support. A logistic regression model was used to identify the predictors.

Results

The results showed that higher educational level, conjugal living, absence of smoking or betel quid chewing, moderate alcohol drinking, routine physical activity, more leisure activities, no hypertension, no diabetes, sleeping well and satisfied with economic condition were the positive predictors for becoming a healthy octogenarian.

Conclusions

Using a multidimensional criterion, the present study identified a list of factors in predicting older Taiwanese becoming healthy octogenarians. The findings highlight the need to identify potential factors for various populations. Many of the predictors are modifiable factors. The present results would be valuable for planning effective health promotion strategies to achieve healthy aging for older adults. Geriatr Gerontol Int 2017; 17: 2579–2585.
Digital image analysis of HER2 immunohistochemistry in gastric‐ and oesophageal adenocarcinoma: a validation study on biopsies and surgical specimens
Authors: Koopman Timco , de Bock Geertruida H , Buikema Henk J , Smits Maria M , Louwen Maarten , Hage Mariska , Imholz Alex L T , van der Vegt Bert
Published: July 2017

Aims

To test the validity of diagnostics incorporating digital image analysis (DIA) for human epidermal growth factor 2 (HER2) immunohistochemistry (IHC) in gastro‐oesophageal adenocarcinomas, as an alternative to current standard diagnostics using manual scoring.

Methods and results

We included 319 consecutive gastro‐oesophageal adenocarcinomas (232 biopsies and 87 surgical specimens). DIA was applied to determine HER2 IHC classification, using both standard breast cancer (BC) and modified gastro‐oesophageal cancer (GEC) cut‐offs. Consensus manual scores were established by four independent observers. Chromogenic in‐situ hybridization (CISH) was performed on all 2+ cases by manual scoring, DIA or both. HER2 status was considered positive in 3+ and CISH‐positive 2+ cases. Overall agreement between DIA and consensus manual scores was 76.5% (weighted κ = 0.66, BC cut‐offs) and 85.6% (weighted κ = 0.80, GEC cut‐offs). Agreement was similar for biopsies and surgical specimens. All disagreement occurred in the manual IHC equivocal cases. DIA resulted in a reduction of 2+ cases: 75.8% with BC cut‐offs and 46.5% with GEC cut‐offs. HER2 status was positive in 48 cases (15%) with standard diagnostics and DIA using GEC cut‐offs, and 46 cases (14.4%) using BC cut‐offs (all with CISH in 2+ cases). Considering standard diagnostics as a reference, DIA showed 93.8% sensitivity and 99.6% specificity (BC cut‐offs) or 97.9% sensitivity and 99.6% specificity (GEC cut‐offs).

Conclusions

DIA is a reliable and feasible alternative to manual HER2 IHC scoring in gastro‐oesophageal adenocarcinoma, both in biopsies and surgical specimens, leading to a reduction of 2+ cases for which subsequent ISH testing is required.
Intratumoral stromal morphometry predicts disease recurrence but not response to 5‐fluorouracil—results from the QUASAR trial of colorectal cancer
Authors: Hutchins Gordon G A , Treanor Darren , Wright Alexander , Handley Kelly , Magill Laura , Tinkler‐Hundal Emma , Southward Katie , Seymour Matthew , Kerr David , Gray Richard , Quirke Philip ,   the QUASAR trial collaborators and the UK National Cancer Research Institute Colorectal Cancer Clinical Studies Group
Published: July 2017

Aims

The biological importance of tumour‐associated stroma is becoming increasingly apparent, but its clinical utility remains ill‐defined. For stage II/Dukes B colorectal cancer (CRC), clinical biomarkers are urgently required to direct therapeutic options. We report here prognostic/predictive analyses, and molecular associations, of stromal morphometric quantification in the Quick and Simple and Reliable (QUASAR) trial of CRC.

Methods and results

Relative proportions of tumour epithelium (PoT) or stroma (PoS) were morphometrically quantified on digitised haematoxylin and eosin (H&E) sections derived from 1800 patients enrolled in QUASAR, which randomised 3239 (91% stage II) CRC patients between adjuvant fluorouracil/folinic acid (FUFA) chemotherapy and observation. The prognostic and predictive values of PoT/PoS measurements were determined by the use of stratified log‐rank analyses. A high proportion of tumour stroma (≥50%) was associated with an increased recurrence risk: 31.3% (143/457) recurrence for ≥50% versus 21.9% (294/1343) for <50% [rate ratio (RR) 1.62; 95% confidence interval (CI) 1.30–2.02; P < 0.0001]. Of patients with stromal proportions of ≥65%, 40% (46/115) had recurrent disease within 10 years. The adverse prognostic effect of a high stromal proportion was independent of established prognostic variables, and was maintained in stage II/Dukes B patients (RR 1.62; 95% CI 1.26–2.08; P = 0.0002). KRAS mutation in the presence of a high stromal proportion augmented recurrence risk (RR 2.93; 95% CI 1.87–4.59; P = 0.0005). Stromal morphometry did not predict response to FUFA chemotherapy.

Conclusions

Simple digital morphometry applied to a single representative H&E section identifies CRC patients with a >50% higher risk of disease recurrence. This technique can reliably partition patients into subpopulations with different risks of tumour recurrence in a simple and cost‐effective manner. Further prospective validation is warranted.
Pyroelectric performance of porous Ba0.85Sr0.15TiO3 ceramics
Authors: Srikanth Keshavmurthy Subramanian , Singh Vinay Pratap , Vaish Rahul
Published: July 2017

Porous Ba0.85Sr0.15TiO3 (BST) ferroelectric ceramics were synthesized by sintering compacts consisting of BST and Poly(methyl methacrylate) (PMMA) (2%, 4%, 6%, 8%, and 10% by wt.) as pore‐forming agent. Systematic investigation of role of porosity on microstructural, dielectric, and pyroelectric performance is carried out. It is observed that as PMMA increased from 0% to 10%, the porosity increased from 7.5% to 29.5%. Dielectric constant decreases and dielectric loss increases with increasing PMMA content at fixed frequency. When porosity reached 29.5%, the relative dielectric constant of the BST composition decreased by more than 78% (from 3500 to 750) at 10 kHz and 300 K. Porosity leads to a significant reduction in dielectric constant and volume‐specific heat capacity, which are of great interest for improving pyroelectric figure of merits (FOMs). Further, the pyroelectric figure of merits for current responsivity (Fi), voltage responsivity (Fv), detectivity (Fd), and energy harvesting (Fe and Fe*) are calculated for all investigated compositions. Compared with dense ceramic, porous BST (10% PMMA) showed an improvement of Fd by 19%, Fe by 61%, and Fe* by 98%. Fv increased by 210% at 300 K/10 kHz. All of these advancements are favorable for the development of pyroelectric devices.
Newborns' Face Recognition: The Role of Facial Movement
Authors: Leo Irene , Angeli Valentina , Lunghi Marco , Dalla Barba Beatrice , Simion Francesca
Published: July 2017

This study investigates the role of dynamic information in identity face recognition at birth. More specifically we tested whether semi‐rigid motion, conveyed by a change in facial expression, facilitates identity recognition. In Experiment 1, two‐day‐old newborns, habituated to a static happy or fearful face (static condition) have been tested using a pair of novel and familiar identity static faces with a neutral expression. Results indicated that newborns manifest a preference for the familiar stimulus, independently of the facial expression. In contrast, in Experiment 2 newborns habituated to a semi‐rigid moving face (dynamic condition) manifest a preference for the novel face, independently of the facial expression. In Experiment 3, a multistatic image of a happy face with different degrees of intensity was utilized to test the role of different amount of static pictorial information in identity recognition (multistatic image condition). Results indicated that newborns were not able to recognize the face to which they have been familiarized. Overall, results clearly demonstrate that newborns' face recognition is strictly dependent on the learning conditions and that the semi‐rigid motion conveyed by facial expressions facilitates identity recognition since birth.