bluuebunny/crossref_metadata_2025 huggingface dataset:
Oral administration of Manuka honey induces IFNγ-dependent resistance to tumor growth that correlates with beneficial modulation of gut microbiota composition
Authors: Masad Razan J. , Idriss Ienas , Mohamed Yassir A. , Al-Sbiei Ashraf , Bashir Ghada , Al-Marzooq Farah , Altahrawi Abeer , Fernandez-Cabezudo Maria J. , Al-Ramadi Basel K.
Published: February 2024

Background

To investigate the potential of Manuka honey (MH) as an immunomodulatory agent in colorectal cancer (CRC) and dissect the underlying molecular and cellular mechanisms.

Methods

MH was administered orally over a 4 week-period. The effect of MH treatment on microbiota composition was studied using 16S rRNA sequencing of fecal pellets collected before and after treatment. Pretreated mice were implanted with CRC cells and followed for tumor growth. Tumors and lymphoid organs were analyzed by flow cytometry (FACS), immunohistochemistry and qRT-PCR. Efficacy of MH was also assessed in a therapeutic setting, with oral treatment initiated after tumor implantation. We utilized IFNγ-deficient mice to determine the importance of interferon signaling in MH-induced immunomodulation.

Results

Pretreatment with MH enhanced anti-tumor responses leading to suppression of tumor growth. Evidence for enhanced tumor immunogenicity included upregulated MHC class-II on intratumoral macrophages, enhanced MHC class-I expression on tumor cells and increased infiltration of effector T cells into the tumor microenvironment. Importantly, oral MH was also effective in retarding tumor growth when given therapeutically. Transcriptomic analysis of tumor tissue highlighted changes in the expression of various chemokines and inflammatory cytokines that drive the observed changes in tumor immunogenicity. The immunomodulatory capacity of MH was abrogated in IFNγ-deficient mice. Finally, bacterial 16S rRNA sequencing demonstrated that oral MH treatment induced unique changes in gut microbiota that may well underlie the IFN-dependent enhancement in tumor immunogenicity.

Conclusion

Our findings highlight the immunostimulatory properties of MH and demonstrate its potential utilization in cancer prevention and treatment.
The significance of Hippo pathway protein expression in oral squamous cell carcinoma
Authors: Amano Yusuke , Matsubara Daisuke , Kihara Atsushi , Yoshimoto Taichiro , Fukushima Noriyoshi , Nishino Hiroshi , Mori Yoshiyuki , Niki Toshiro
Published: February 2024

Introduction

The Hippo pathway consists of mammalian sterile 20-like kinase 1/2 (MST1/2), large tumor suppressor 1/2 (LATS1/2), and yes-associated protein (YAP)1. Herein, we present the first report on the significance of major Hippo pathway protein expression in oral squamous cell carcinoma (OSCC).

Methods

The analyses included oral epithelial dysplasia (OED, n = 7), carcinoma in situ (CIS, n = 14), and oral squamous cell carcinoma (OSCC, n = 109).

Results

Cytoplasmic expression of MST1, LATS1, and LATS2 was low in OED, CIS, and OSCC. The cytoplasmic expression of MST2 was high in OED (5/7 cases), CIS (9/14 cases), and poorly differentiated OSCC (8/8 cases) but was low/lost in a proportion of differentiated OSCC (60/101 cases). The expression of YAP1 was associated with differentiation; low YAP expression was significantly more frequent in well-differentiated OSCC (35/71 cases), compared to moderately and poorly differentiated OSCC (11/38 cases). An infiltrative invasion pattern was associated with a high expression of MST2 and high expression of YAP1. The high expression of YAP1 was associated with features of epithelial-to-mesenchymal transition (EMT), such as the loss of E-cadherin and high expression of vimentin, laminin 5, and Slug. High expression of protein arginine methyltransferase (PRMT) 1 or 5, which positively regulates YAP activity, was associated with the high expression of YAP1 (p < 0.0001).

Conclusion

Among the major Hippo pathway proteins, MST2 displayed a distinctive expression pattern in a significant proportion of differentiated OSCC, suggesting a possible differential role for MST2 depending on the course of OSCC progression. A high YAP1 expression may indicate aggressive OSCC with EMT via PRMTs at the invasive front.
Assembly processes of rhizosphere and phyllosphere bacterial communities in constructed wetlands created via transformation of rice paddies
Authors: Deng Nan , Liu Caixia , Tian Yuxin , Song Qingan , Niu Yandong , Ma Fengfeng
Published: February 2024

Constructed wetlands are an efficient and cost-effective method of restoring degraded wetlands, in which the microorganisms present make a significant contribution to the ecosystem. In this study, we comprehensively investigated the patterns of diversity and assembly processes of 7 types of constructed wetlands at the rhizosphere and phyllosphere levels. The results showed that the rhizosphere communities of the constructed wetlands exhibited a more balanced structure than that of paddy fields, and 5 types of constructed wetland demonstrated higher potential diversity than that of paddy fields. However, the opposite trend was observed for the phyllosphere communities. Analysis of mean nearest taxon difference indicated that both deterministic and stochastic processes affected the establishment of the rhizosphere and phyllosphere communities, and stochastic processes may have had a larger effect. An iCAMP model showed that dispersal limitation was the most important factor (67% relative contribution) in the rhizosphere community, while drift was the most important (47% relative contribution) in the phyllosphere community. Mantel tests suggested that sucrase, average height, top height, total biomass, belowground biomass, maximum water-holding capacity, and capillary porosity were significantly correlated with processes in the rhizosphere community, whereas factors such as the deterministic process, average height, top height, and SOC were significantly correlated with deterministic processes in the phyllosphere community. Our results can assist in the evaluation of artificial restorations, and can provide understanding of the ecological processes of microbial communities, as well as new insights into the manipulation of microorganisms in polluted wetland ecosystems.
Precision needle-punch tumor enrichment from paraffin blocks improves the detection of clinically actionable genomic alterations and biomarkers
Authors: Lin Douglas I. , Huang Richard S. P. , Ladas Ioannis , Keller Rachel B. , Patel Nimesh R. , Lakis Sotirios , Decker Brennan , Janovitz Tyler , Mata Douglas A. , Ross Jeffrey S. , Vergilio Jo-Anne , Elvin Julia A. , Herbst Roy S. , Mack Philip C. , Killian Jonathan K.
Published: February 2024

Background

While many molecular assays can detect mutations at low tumor purity and variant allele frequencies, complex biomarkers such as tumor mutational burden (TMB), microsatellite instability (MSI), and genomic loss of heterozygosity (gLOH) require higher tumor purity for accurate measurement. Scalable, quality-controlled, tissue-conserving methods to increase tumor nuclei percentage (TN%) from tumor specimens are needed for complex biomarkers and hence necessary to maximize patient matching to approved therapies or clinical trial enrollment. We evaluated the clinical utility and performance of precision needle-punch enrichment (NPE) compared with traditional razor blade macroenrichment of tumor specimens on molecular testing success.

Methods

Pathologist-directed NPE was performed manually on formalin-fixed, paraffin embedded (FFPE) blocks. Quality control of target capture region and quantity of residual tumor in each tissue block was determined via a post-enrichment histologic slide recut. Resultant tumor purity and biomarker status were determined by the computational analysis pipeline component of the FDA-approved next-generation sequencing (NGS) assay, FoundationOne®CDx. Following NPE implementation for real-world clinical samples, assay performance and biomarker (MSI, TMB, gLOH) detection were analyzed.

Results

In real-world clinical samples, enrichment rate via NPE was increased to ~50% over a 2.5-year period, exceeding the prior use of razor blade macro-enrichment (<30% of cases) prior to NPE implementation due to proven efficacy in generating high quality molecular results from marginal samples and the ease of use for both pathologist and histotechnologists. NPE was associated with lower test failures, higher computational tumor purity, and higher rates of successful TMB, MSI and gLOH determination when stratified by pre-enriched (incipient) tumor nuclei percentage. In addition, challenging cases in which tumor content was initially insufficient for testing were salvaged for analysis of biomarker status, gene amplification/deletion, and confident mutant or wild-type gene status determination.

Conclusions

Pathologist-directed precision enrichment from tissue blocks (aka NPE) increases tumor purity, and consequently, yields a greater number of successful tests and complex biomarker determinations. Moreover, this process is rapid, safe, inexpensive, scalable, and conserves patient surgical pathology material. NPE may constitute best practice with respect to enriching tumor cells from low-purity specimens for biomarker detection in molecular laboratories.
Attenuation of intestinal ischemia-reperfusion-injury by anesthetics: a potentially protective effect of anesthetic management in experimental studies
Authors: Huang Zhan , Bai Yiping , Chen Ying , Chen Ye , Jiang Yuan , Zhou Jun
Published: February 2024

Intestinal ischemia-reperfusion injury (IRI) is a potentially severe clinical syndrome after major surgical procedures. In addition to causing intestinal mucosa injury, intestinal IRI further damages distant organs, causing the severity of the condition in patients. So far, effective therapy for intestinal IRI is still absent, and the survival rate of the patients is low. Previous experimental studies have shown that some anesthetics can alleviate intestinal IRI and protect organs while exerting their pharmacological effects, indicating that reasonable perioperative anesthesia management may provide potential benefits for patients to avoid intestinal IRI. These meaningful findings drive scholars to investigate the mechanism of anesthetics in treating intestinal IRI in-depth to discuss the possible new clinical uses. In the present mini-review, we will introduce the protective effects of different anesthetics in intestinal IRI to help us enrich our knowledge in this area.
Development of axion haloscopes for high-mass search at CAPP
Authors: Youn SungWoo , Jeong Junu , Semertzidis Yannis K.
Published: February 2024

The axion offers a well-motivated solution to two fundamental questions in modern physics: the strong CP problem and the dark matter mystery. Cavity haloscopes, exploiting resonant enhancement of photon signals, provide the most sensitive searches for axion dark matter in the microwave region. However, current experimental sensitivities are limited to the O(100)μeV range, while recent theoretical predictions for the axion mass favor up to O(102)μeV, suggesting the need of new experimental approaches that are suitable for higher mass regions. CAPP has developed/proposed several haloscopes effective for high-mass axion searches based on new cavity concepts and practical tuning mechanisms. They are characterized by large detection volumes and/or high quality factors at high frequencies, achieved by partitioning a single cavity into multiple cells, exploiting higher-order resonant modes, and constructing dielectric photonic crystal structures. Improving on the dish antenna haloscope scheme, a horn antenna array has also been proposed for volume-efficient broadband search in the THz region. We review these haloscope designs for sensitive search in the high-mass regions and discuss their impacts on future experiments.
Characteristics of bacterial communities in rhizosphere and bulk soil in Fe-deficient citrus growing in coastal saline-alkali land
Authors: Jiang Tianchi , Chen Jiuzhou , Huang Yu , Chang Xiaoyan , Wu Yuping , Liu Gaoping , Wang Runze , Xu Kuan , Lu Lingli , Lin Haizhong , Tian Shengke
Published: February 2024

Aims

Citruses often occur with imbalance in iron nutrition in coastal saline-alkali lands, which severely limits the yield and quality of the fruit. In the rhizosphere, the salt content plays a crucial role in reducing uptake of iron, as well as the activity and abundance of bacteria. However, few studies have explored how salt content affects the effectiveness of iron and the community structure of bacteria across different vertical spatial scales.

Methods

We investigated the citrus rhizosphere (0–30 cm) and bulk (0–60 cm) soil microenvironments of the coastal saline soil were analyzed using the 16S rRNA amplicon and inductively coupled plasma-optical emission spectroscopy.

Results

We found that the nutrient-related elements in the rhizosphere and bulk soil decreased with increasing soil depth, while the salinity-related elements showed the opposite trend. The nutrient-related element content in the rhizosphere was higher than that in the bulk, whereas the salinity-alkaline-related element content was lower than that in the bulk. The structure and diversity of bacterial communities are affected by the rhizosphere and soil depth. In the bulk, there are enriched bacteria such as WB1-A12, Nitrospiraceae and Anaerolineae that are tolerant to salt-alkali stress. In the rhizosphere, bacteria that promote plant nutrient absorption and secretion of iron carriers, such as Pseudomonas, Streptomyces, and Duganella, are prominent.

Conclusions

The soil depth and rhizosphere affect soil nutrients and saline alkali-related factors. Changes in soil depth and rhizosphere determine the structure and diversity of bacterial communities. Rhizosphere enhances iron absorption promoting bacteria to alleviate iron deficiency stress in saline-alkali soils. Our results indicate that citrus roots maybe can resist the stress of iron deficiency in saline-alkali soils by enhancing iron absorption promoting bacteria.
Plant virus diversity in bee and pollen samples from apple (Malus domestica) and sweet cherry (Prunus avium) agroecosystems
Authors: Smadi Malek , Lee Eunseo , Phelan James , Wang Aiming , Bilodeau Guillaume J. , Pernal Stephen F. , Guarna M. Marta , Rott Mike , Griffiths Jonathan S.
Published: February 2024

Introduction

Honey bee (Apis mellifera) pollination is widely used in tree fruit production systems to improve fruit set and yield. Many plant viruses can be associated with pollen or transmitted through pollination, and can be detected through bee pollination activities. Honey bees visit multiple plants and flowers in one foraging trip, essentially sampling small amounts of pollen from a wide area. Here we report metagenomics-based area-wide monitoring of plant viruses in cherry (Prunus avium) and apple (Malus domestica) orchards in Creston Valley, British Columbia, Canada, through bee-mediated pollen sampling.

Methods

Plant viruses were identified in total RNA extracted from bee and pollen samples, and compared with profiles from double stranded RNA extracted from leaf and flower tissues. CVA, PDV, PNRSV, and PVF coat protein nucleotide sequences were aligned and compared for phylogenetic analysis.

Results

A wide array of plant viruses were identified in both systems, with cherry virus A (CVA), prune dwarf virus (PDV), prunus necrotic ringspot virus (PNRSV), and prunus virus F (PVF) most commonly detected. Citrus concave gum associated virus and apple stem grooving virus were only identified in samples collected during apple bloom, demonstrating changing viral profiles from the same site over time. Different profiles of viruses were identified in bee and pollen samples compared to leaf and flower samples reflective of pollen transmission affinity of individual viruses. Phylogenetic and pairwise analysis of the coat protein regions of the four most commonly detected viruses showed unique patterns of nucleotide sequence diversity, which could have implications in their evolution and management approaches. Coat protein sequences of CVA and PVF were broadly diverse with multiple distinct phylogroups identified, while PNRSV and PDV were more conserved.

Conclusion

The pollen virome in fruit production systems is incredibly diverse, with CVA, PDV, PNRSV, and PVF widely prevalent in this region. Bee-mediated monitoring in agricultural systems is a powerful approach to study viral diversity and can be used to guide more targeted management approaches.
Yield prediction in a peanut breeding program using remote sensing data and machine learning algorithms
Authors: Pugh N. Ace , Young Andrew , Ojha Manisha , Emendack Yves , Sanchez Jacobo , Xin Zhanguo , Puppala Naveen
Published: February 2024

Peanut is a critical food crop worldwide, and the development of high-throughput phenotyping techniques is essential for enhancing the crop’s genetic gain rate. Given the obvious challenges of directly estimating peanut yields through remote sensing, an approach that utilizes above-ground phenotypes to estimate underground yield is necessary. To that end, this study leveraged unmanned aerial vehicles (UAVs) for high-throughput phenotyping of surface traits in peanut. Using a diverse set of peanut germplasm planted in 2021 and 2022, UAV flight missions were repeatedly conducted to capture image data that were used to construct high-resolution multitemporal sigmoidal growth curves based on apparent characteristics, such as canopy cover and canopy height. Latent phenotypes extracted from these growth curves and their first derivatives informed the development of advanced machine learning models, specifically random forest and eXtreme Gradient Boosting (XGBoost), to estimate yield in the peanut plots. The random forest model exhibited exceptional predictive accuracy (R2 = 0.93), while XGBoost was also reasonably effective (R2 = 0.88). When using confusion matrices to evaluate the classification abilities of each model, the two models proved valuable in a breeding pipeline, particularly for filtering out underperforming genotypes. In addition, the random forest model excelled in identifying top-performing material while minimizing Type I and Type II errors. Overall, these findings underscore the potential of machine learning models, especially random forests and XGBoost, in predicting peanut yield and improving the efficiency of peanut breeding programs.
“Trust people you’ve never worked with” – A social network visualization of teamwork, cohesion, social support, and mental health in NHS Covid personnel
Authors: Schilling Stefan , Armaou Maria , Morrison Zoe , Carding Paul , Bricknell Martin , Connelly Vincent
Published: February 2024

Background

The unprecedented rapid re-deployment of healthcare workers from different care pathways into newly created and fluid COVID-19 teams provides a unique opportunity to examine the interaction of many of the established non-technical factors for successful delivery of clinical care and teamwork in healthcare settings. This research paper therefore aims to address these gaps by qualitatively exploring the impact of COVID work throughout the pandemic on permanent and deployed personnel’s experiences, their ability to effectively work together, and the effect of social dynamics (e.g., cohesion, social support) on teamwork and mental health.

Methods

Seventy-five interviews were conducted across the UK between March and December 2021 during wave 2 and 3 of COVID-19 with 75 healthcare workers who were either permanent staff on Intensive Care/High Dependency Units used as COVID wards, had been rapidly deployed to such a ward, or had managed such wards. Work Life Balance was measured using the WLB Scale. Interview transcripts were qualitatively coded and thematic codes were compared using network graph modeling.

Results

Using thematic network analysis, four overarching thematic clusters were found, (1) teamwork, (2) organizational support and management, (3) cohesion and social support, and (4) psychological strain. The study has three main findings. First, the importance of social factors for teamwork and mental health, whereby team identity may influence perceptions of preparedness, collaboration and communication, and impact on the collective appraisal of stressful events and work stressors. Secondly, it demonstrates the positive and negative impact of professional roles and skills on the development of teamwork and team identity. Lastly the study identifies the more pronounced negative impact of COVID work on deployed personnel’s workload, mental health, and career intentions, exacerbated by reduced levels of social support during, and after, their deployment.

Conclusion

The thematic network analysis was able to highlight that many of the traditional factors associated with the successful delivery of patient care were impeded by pandemic constraints, markedly influencing personnel’s ability to work together and cope with pandemic work stressors. In this environment teamwork, delivery of care and staff well-being appear to depend on relational and organizational context, social group membership, and psycho-social skills related to managing team identity. While results hold lessons for personnel selection, training, co-location, and organizational support during and after a pandemic, further research is needed into the differential impact of pandemic deployment on HCWs mental health and teamwork.
Faculty versus students: different perceptions of misconducts at university
Authors: Alcalde-Fradejas Nuria , Marzo-Navarro Mercedes , Ramírez-Alesón Marisa
Published: February 2024

Introduction

Academic integrity is a goal to be achieved by university institutions, and student academic behavioral misconduct is a phenomenon to be fought and eradicated. Two of the main problems faced by universities in this area are: (1) the lack of consensus among students and faculty on the seriousness of acts considered academic misconduct; and (2) the difficulty of noticing and controlling certain student behaviors. The main aim of this paper is to assess the importance of these two problems.

Methods

For this purpose, the authors compare, on the one hand, students’ and teachers’ perceptions of the seriousness of different types of dishonest and inappropriate behaviors and, on the other hand, the frequency with which they report that these misconducts occur. Two samples were taken from the responses of students and teachers of the Economics and Business School of the University of Zaragoza. The first consisted of 333 students and the second of 72 teachers. The academic misconducts asked about were grouped into three categories: academic works, exams, and interpersonal relationships in the classroom. Nonparametric tests were used to study the significance of the differences observed in the responses of students and teachers.

Results

Results show that the greatest differences in the assessment of the seriousness of academic misconducts are in the group referring to interpersonal relationships. In terms of frequency, the study reveals that there is a serious problem of moral hazard in some of the behaviors analyzed, since the frequency with which teachers notice these practices is lower than that expressed by students.

Discussion

Based on these results, possible measures to be adopted in universities in order to eradicate the academic misconduct problem are discussed.
Effects of COVID-19 on Autism Spectrum Disorder in Qatar
Authors: Alshaban Fouad A. , Ghazal Iman , Al-Harahsheh Sanaa T. , Lotfy Mustafa , Al-Shammari Hawraa , Al-Faraj Fatema , Thompson I. Richard , Ratto Allison B. , Nasir Assal , Tolefat Mohamed
Published: February 2024

Introduction

The unprecedented impact of the coronavirus pandemic (COVID-19) has had profound implications on the ASD community, including disrupting daily life, increasing stress and emotional dysregulation in autistic children, and worsening individual and family well-being.

Methods

This study used quantitative and qualitative survey data from parents in Qatar (n=271), to understand the impact of the COVID-19 pandemic on autistic children and their families in Qatar. The questionnaire was a combination of open-ended (qualitative) and closed-ended (quantitative) questions to explore patterns in the experiences of the different families, as well as to contrive themes. The survey was created in a way to evaluate the psychological, academic/intervention, economic, and other impacts of the pandemic related measures on a sample of multicultural families residing in the State of Qatar during the peak period of confinement and physical distancing in 2020. Data acquisition involved the utilization of Google Forms. Subsequent quantitative analysis employed the SPSS software and chi-square analysis for numerical examination, enabling the characterization of the studied population and exploration of associations between parental stress levels and variables such as employment status, therapy accessibility, presence of hired assistance, and alterations in their childs skills. Concurrently, qualitative data from written responses underwent thorough categorization, encompassing themes such as emotional isolation, mental or financial challenges, and difficulties in obtaining support.

Results

Parents expressed distress and disturbance in their daily lives, including profound disruptions to their childrens access to treatment, education, and activities. Most parents reported deteriorations in their childrens sleep (69.4%), behavioral regulation (52.8%), and acquired skills across multiple domains (54.2%). Parents also reported decreased access to family and social support networks, as well as decreased quality of clinical and community support. Qualitative analysis of parental responses revealed that child developmental regression was an important source of parental stress.

Discussion and conclusion

The greater impact of the pandemic on autistic children and their families emphasizes the need for accessible and affordable health, education, and family services to manage their special needs.
Are carfentanil and acrylfentanyl naloxone resistant?
Authors: Feasel Michael G. , Moran Theodore S. , Cheng Boyle C. , Averick Saadyah
Published: February 2024

The rapid rise in deaths since 2012 due to opioid poisoning is correlated with the proliferation of potent synthetic opioid agonists such as fentanyl, acrylfentanyl, and carfentanil. The efficacy of frontline antidotes such as naloxone in reversing such poisoning events has been questioned, and the possibility of naloxone-resistant synthetic opioids has been raised. In this manuscript, we applied in vitro techniques to establish the median effective inhibitory concentrations for fentanyl, acrylfentanyl, and carfentanil and subsequently evaluate naloxone’s ability to reverse agonist–receptor interactions.
A study on the factors influencing the intention to receive booster shots of the COVID-19 vaccine in China based on the information frame effect
Authors: Zhu Qizhen , Gao Yunyun , Hu Qingyuan , Hu DeHua , Wu Xusheng
Published: February 2024

Introduction

In the response to and prevention and control of the Novel coronavirus pneumonia, the COVID-19 vaccine does not provide lifelong immunity, and it is therefore important to increase the rate of booster shots of the COVID-19 vaccine. In the field of information health science, research has found that information frames have an impact in changing individual attitudes and health behaviors.

Objective

This study focuses on the effects of different influencing factors on the public’s willingness to receive the booster shots of the COVID-19 vaccine under two information frameworks.

Methods

An online questionnaire was conducted to explore the effects of demographic characteristics, personal awareness, social relationships, risk disclosure, perceived booster vaccination protection rate, and duration of protection under the assumption of an information framework. T test and one-way analysis were used to testing the effect of variables.

Results

(1) The persuasion effect under the gain frame is higher than that under the loss frame (B = 0.863 vs. B = 0.746); (2) There was no significant difference in subjects’ intention of booster vaccination in terms of gender, age, income, occupation, educational background and place of residence. Whether family members received booster vaccination was strongly correlated with their intention of vaccination under the loss framework (p = 0.017, M = 4.63, SD = 0.664). (3) The higher the understanding of COVID-19, the higher the degree of compliance with the government’s COVID-19 prevention and control measures, and the higher the willingness to strengthen vaccination; (4) Risk disclosure has a significant impact on people’s willingness to receive COVID-19 booster shots (M = 2.48, under the loss framework; M = 2.44, under the gain framework); (5) Vaccine protection rate and duration of protection have an impact on people’s willingness to vaccinate. Increased willingness to vaccinate when the protection rate of booster vaccine approaches 90% (M = 4.76, under the loss framework; M = 4.68, under the gain framework). When the vaccine protection period is 2 years, people are more willing to receive a booster vaccine; and the willingness to receive a booster shot is stronger under the loss framework (M = 4.60, SD = 0.721, p = 0.879).

Conclusion

The impact of the information framework on COVID-19 vaccination intentions is different, and the disclosure of relevant health information should focus on the impact of the information framework and content on the public’s behavior toward strengthening vaccination. Therefore, in the face of public health emergencies, public health departments, healthcare institutions, and other sectors can consider adopting the Gainful Information Framework tool to disseminate health information to achieve better persuasion and promote public health behavior change enhancing public health awareness, and promoting universal vaccination.
The relationship between sense of coherence and emotional intelligence as individual health assets for mental health promotion in students and healthcare professionals: a scoping review
Authors: Urtubia-Herrera Valentina , Navarta-Sánchez María Victoria , Palmar-Santos Ana María , Pedraz-Marcos Azucena , García-Gomez Alberto , Luis Elkin O. , Bermejo-Martins Elena
Published: February 2024

Introduction

Workplace Mental health promotion in healthcare sector, is a global priority due to the stress associated with caregiving environments and the increase of mental health problems among health professionals and students. The role of emotional intelligence (EI) and sense of coherence (SOC) have been identified as critical health protectors. However, the relationship between them as well as the underlying mechanisms of these relationships on health benefits in this population is still unclear.

Aim

To synthetize the existing literature on the relationship between emotional intelligence and sense of coherence, as well as their mutual impact on healthcare workers’ and student’s well-being.

Method

A scoping review was conducted following the Joanna Briggs Institute guidelines. A systematic search was conducted in PsyCINFO, CINHAL, SCOPUS and PUBMED databases, using key-terms such as students, health professionals, emotional intelligence, and sense of coherence.

Results

A total of 11 articles were included, with a range of years from 2014 to 2022. Evidence was found to support the positive relationship between sense of coherence and emotional intelligence. The use of EI as a training pathway to improve SOC and health promoting behaviors is suggested. The benefits of intervening on these factors contribute to improved health professionals’ and students’ general well-being and motivation for a better performance, either in their studies or clinical work.

Conclusion

The positive relationship between emotional intelligence and a sense of coherence has direct and indirect benefits on students’ and healthcare professionals’ well-being. Future studies should address longitudinal and experimental analysis to confirm these findings.
Post-COVID-19 pandemic organ donation activities in Germany: a multicenter retrospective analysis
Authors: Englbrecht Jan Sönke , Schrader Daniel , Alders Jörg Benedikt , Schäfer Melanie , Soehle Martin
Published: February 2024

Introduction

The COVID-19 pandemic had a negative impact on the number of solid organ transplantations. After a global decline of 16% in 2020, their numbers subsequently returned to pre-pandemic levels. In contrast, numbers in Germany remained almost constant in 2020 and 2021 but fell by 6.9% in 2022. The reasons for this divergent development are unknown.

Methods

The number of deceased with a severe brain damage, potential and utilized donors after braindeath and the intensive care unit treatment capacity were retrospectively compared for the years 2022 and 2021 at five university hospitals in North Rhine-Westphalia, Germany. Reasons for a donation not utilized were reviewed. To enable a comparison of the results with the whole of Germany and the pre-pandemic period, numbers of potential and utilized donors were extracted from official organ donation activity reports of all harvesting hospitals in Germany for the years 2019–2022.

Results

The numbers of deceased with a severe brain damage (−10%), potential (−9%), and utilized donors after braindeath (−44%), and intensive care unit treatment capacities (−7.2%) were significantly lower in 2022 than 2021. A COVID-19 infection was a rarer (−79%), but donor instability (+44%) a more frequent reason against donation in 2022, whereas preserved brain stem reflexes remained the most frequent reason in both years (54%). Overall numbers of potential and utilized donations in Germany were lower in 2022 than in the pre-pandemic period, but this was mainly due to lower numbers in hospitals of lower care. The number of potential donors in all university hospitals were higher in 2022 but utilized donations still lower than in 2019.

Conclusion

The decrease in potential and utilized donations was a result of reduced intensive care unit treatment capacities and a lower conversion rate at the five university hospitals. A COVID-19 infection did not play a role in 2022. These results indicate that ICU treatment capacities must be restored to increase donations. The lower number of potential donors and the even lower conversion rate in 2022 throughout Germany show that restructuring the organ procurement process in Germany needs to be discussed to increase the number of donations.
Practical guidance to identify and troubleshoot suboptimal DSC-MRI results
Authors: Prah Melissa A. , Schmainda Kathleen M.
Published: February 2024

Relative cerebral blood volume (rCBV) derived from dynamic susceptibility contrast (DSC) perfusion MR imaging (pMRI) has been shown to be a robust marker of neuroradiological tumor burden. Recent consensus recommendations in pMRI acquisition strategies have provided a pathway for pMRI inclusion in diverse patient care centers, regardless of size or experience. However, even with proper implementation and execution of the DSC-MRI protocol, issues will arise that many centers may not easily recognize or be aware of. Furthermore, missed pMRI issues are not always apparent in the resulting rCBV images, potentiating inaccurate or missed radiological diagnoses. Therefore, we gathered from our database of DSC-MRI datasets, true-to-life examples showcasing the breakdowns in acquisition, postprocessing, and interpretation, along with appropriate mitigation strategies when possible. The pMRI issues addressed include those related to image acquisition and postprocessing with a focus on contrast agent administration, timing, and rate, signal-to-noise quality, and susceptibility artifact. The goal of this work is to provide guidance to minimize and recognize pMRI issues to ensure that only quality data is interpreted.
Illegitimate Tasks and Work–Family Conflict as Sequential Mediators in the Relationship between Work Intensification and Work Engagement
Authors: Andrade Cláudia , Neves Paula C.
Published: February 2024

Organizational restructuring and technological developments have been associated with working for longer hours, assuming more responsibilities, and feeling more pressure to reach demanding work targets leading to work intensification. Guided by the framework of the JD Resources and stress-as-offense-to-self theory, the aim of the present study was to explore how work intensification decreases work engagement. We posit that illegitimate tasks and work–family conflict could play a mediator role between work intensification and work engagement. A total of 480 employees in Portugal completed an online survey. The results showed a negative correlation between work intensification, illegitimate tasks, and work–family conflict. Work identification can affect work engagement indirectly through two paths: the separate intermediary effect of illegitimate tasks or work–family conflict and the continuous mediating role of illegitimate tasks and work–family conflict. The results indicate that work intensification decreases work engagement through the role of illegitimate tasks and work–family conflict. This study contributes to the literature on the impacts of work intensification by considering the sequential mediating effect of illegitimate tasks and work–family conflict on work engagement. Although past research has claimed that work intensification negatively impacts work engagement, our results showed that illegitimate tasks and work–family conflict play a critical role in this process.
Enhancing Subsurface Soil Moisture Forecasting: A Long Short-Term Memory Network Model Using Weather Data
Authors: Basir Md. Samiul , Noel Samuel , Buckmaster Dennis , Ashik-E-Rabbani Muhammad
Published: February 2024

Subsurface soil moisture is a primary determinant for root development and nutrient transportation in the soil and affects the tractability of agricultural vehicles. A statistical forecasting model, Vector AutoRegression (VAR), and a Long Short-Term Memory network (LSTM) were developed to forecast the subsurface soil moisture at a 20 cm depth using 9 years of historical weather data and subsurface soil moisture data from Fort Wayne, Indiana, USA. A time series analysis showed that the weather data and soil moisture have a stationary seasonal tendency and demonstrated that soil moisture can be forecasted from weather data. The VAR model estimates volumetric soil moisture of one-day ahead with an R2, MAE (m3m−3), MSE (m6m−6), and RMSE (m3m−3) of 0.698, 0.0561, 0.0046, and 0.0382 for 2021 corn cropping season, whereas the LSTM model using inputs of previous seven days yielded R2, MAE (m3m−3), MSE (m6m−6), and RMSE (m3m−3) of 0.998, 0.00237, 0.00002, and 0.00382, respectively as tested for cropping season of 2020 and 0.973, 0.00368, 0.00003 and 0.00577 as tested for the cropping season of 2021. The LSTM model presents a viable data-driven alternative to traditional statistical models for forecasting subsurface soil moisture.
Lateral Overturning and Backward Rollover of Agricultural Tractors: A Review
Authors: Jang Moon-Kyeong , Kim Seung-Jun , Shin Beom-Soo , Nam Ju-Seok
Published: February 2024

Tractor accidents caused by lateral overturning and backward rollover during agricultural activities and general driving are common. In this study, various research cases were analyzed to identify the factors influencing the lateral overturning and backward rollover of tractors and to examine their static and dynamic stability. Studies on the analysis of the major causes of these incidents and evaluation of tractor safety were compiled. Test methods, including actual tests and simulations, were categorized, and the characteristics of lateral overturning and backward rollover safety of tractors in different studies were examined. Additionally, safety improvement measures were proposed by identifying and summarizing the causes of accidents involving agricultural machinery. Tractor safety was evaluated primarily by conducting actual tractor and simulation tests. These tests were classified into field tests, tests on scale models, spreadsheet programs, and 3D simulation programs. The primary causes of lateral overturning and backward rollover were unstable center of gravity, extremely high driving speed, and ground conditions. Given the considerable number of studies dedicated to evaluating tractor safety, various technologies aimed at preventing lateral overturning and backward rollover incidents are expected to be applied to tractors in the future. The production and testing of safe agricultural machinery are expected to contribute to a reduction in accident rates.
Increasing Planting Density and Optimizing Irrigation to Improve Maize Yield and Water-Use Efficiency in Northeast China
Authors: Shen Dongping , Wang Keru , Zhou Linli , Fang Liang , Wang Zhen , Fu Jiale , Zhang Tingting , Liang Zhongyu , Xie Ruizhi , Ming Bo , Hou Peng , Xue Jun , Li Jianmin , Kang Xiaojun , Zhang Guoqiang , Li Shaokun
Published: February 2024

We investigated the effects of variety, planting density, and irrigation amount on grain yield, water-use efficiency (WUE), and evapotranspiration (ETc). The trial was conducted in Tong Liao, Inner Mongolia, from 2021 to 2022, with compact variety Dika159 (DK159) and conventional variety Zhengdan958 (ZD958) as the test materials. The planting density was set to 6.0 × 104 plants/ha (D1, local farmer planting density) and 9.0 × 104 plants/ha (D2), with five irrigation levels: 450 mm (W450, irrigation amount used by local farmers, CK); 360 mm (W360); 270 mm (W270); 180 mm (W180); and 90 mm (W90). The results indicate that the yield and WUE of variety DK159 increased by 7.48% and 5.00%, compared to ZD958, respectively. Increasing planting density enhanced yield by 13.32–15.57% in maize yield and 9.55–11.47% in WUE. Maize yield exhibited a trend of increasing linearly with the irrigation amount before reaching a plateau, reaching a maximum (16.62–17.39 t/ha) and high WUE (2.45–2.49 kg/m3) with DK159-D2-W270. The highest water consumption intensity occurred during the silking stage to the milk stage for different densities and varieties. The results indicate that selecting compact varieties, increasing planting density, and optimizing irrigation amount through integrated drip irrigation and water fertilizer can effectively improve maize yield and WUE.
Nutritional Enhancement of Polimaize Lines: Integrating Native Mexican Maize Alleles into High-Yield Varieties
Authors: Oyoque-Salcedo Guadalupe , Arias-Martínez Sergio , Gutiérrez-Cárdenas Oscar Giovanni , Montañez-Soto José Luis , Oregel-Zamudio Ernesto , Torres-García Jesús Rubén
Published: February 2024

In this study, we evaluated Polimaize lines, named Polimaize, through the integration of alleles from the Native Blue Corn (NBC-JIQ), a local Mexican maize variety indigenous to the northeast region of Michoacán, Mexico, into elite maize lines curated by the International Maize and Wheat Improvement Center (CIMMYT). This crossbreeding aimed to enhance the nutritional profile of maize, particularly in terms of antioxidants and anthocyanins, which are scarce in elite lines. Our results demonstrated a significant increase in these compounds in the Polimaize lines, with variations due to heterosis. Despite these nutritional improvements, some traits showed decreased concentrations compared to parent lines, notably in sucrose and tryptophan, suggesting a potential trade-off. The study also found significant heritability in amino acids and tryptophan, while hexose sugars showed no substantial heritability. The Polimaize variety exhibited high starch content heritability, comparable to elite lines. Field trials confirmed Polimaize’s promising yield and agronomic traits, highlighting its potential for enhancing consumer health and contributing to sustainable agriculture through enriched crop nutritional quality. This project underscores the value of integrating local race alleles into elite lines, offering genetic diversity in maize cultivation.
Overexpressing ATP Sulfurylase Improves Fe-Deficiency Tolerance in Apple Calli and Tobacco
Authors: Cheng Jiao , Zhang Zhongxing , Gao Yanlong , Dong Yongjuan , Xian Xulin , Li Cailong , Ding Liang , Wang Yanxiu
Published: February 2024

Iron (Fe) deficiency is one of the most common micronutrient deficiencies limiting crop production globally, especially in arid regions due to decreased availability of Fe in alkaline soils. The ATP sulfurylase (ATPS) gene has been reported to participate in regulating various abiotic stresses. Transcriptome data and qRT-PCR analysis revealed that the ATP sulfurylase gene MhATPS1 was notably induced by Fe-deficiency stress. Consequently, MhATPS1 (103410737) was isolated from Malus halliana, and transgenic tobacco and transgenic apple calli were successfully obtained by genetic transformation. Compared with the wild type (WT), transgenic MhATPS1 lines (transgenic tobacco and transgenic apple calli) displayed stronger resistance to Fe-deficiency treatment. To be specific, transgenic plants exhibited better growth, accumulated more Fe2+ content, had higher ferric chelate reductase (FCR) activity, and a greater active oxygen scavenging capacity. Furthermore, transgenic MhATPS1 lines up-regulated the expression of Fe uptake genes under Fe-deficit stress. Additionally, MhATPS1 transgenic lines secreted more H+ content compared to the WT. In summary, these findings indicate that the MhATPS1 gene may play a positive role in Fe-deficiency stress in both tobacco and apple calli.
Prehispanic Arid Zone Farming: Hybrid Flood and Irrigation Systems along the North Coast of Peru
Authors: Caramanica Ari
Published: February 2024

As arid lands expand across the globe, scholars increasingly turn to the archaeological record for examples of sustainable farming in extreme environments. The arid north coast of Peru was the setting of early and intensive irrigation-based farming; it is also periodically impacted by sudden, heavy rainfall related to the El Niño Southern Oscillation (ENSO) phenomenon. While the sociopolitical effects, technologies, and engineering expertise of these irrigation systems have been thoroughly examined and theorized, little is known about how farmers managed periods of water stress. The aim of this study is to test whether arid zone farming was supported by hybrid, intermittent flood and perennial water source systems in the prehispanic past. An arroyo in the Chicama Valley was selected for preliminary data collection, and these data are presented here: (1) drone photography of the arroyo capturing the aftermath of a recent (2023) rain event; and (2) potassium (K) soil test kit results from samples collected near suspected prehispanic check dam features in the same area. The paper combines these data with comparative examples from the literature to suggest that the prehispanic features functioned as water-harvesting infrastructure. The paper concludes that sustainable, arid zone farming can be supported by hybrid, intermittent flood and perennial water source systems.