bluuebunny/crossref_metadata_2025 huggingface dataset:
Biological Activities of Some Isoquinoline Alkaloids from Fumaria schleicheri Soy. Will.
Authors: Păltinean Ramona , Ielciu Irina , Hanganu Daniela , Niculae Mihaela , Pall Emoke , Angenot Luc , Tits Monique , Mocan Andrei , Babotă Mihai , Frumuzachi Oleg , Tămaş Mircea , Crişan Gianina , Frederich Michel
Published: May 2022

Fumaria schleicheri Soy. Will. is a species belonging to the Papaveraceae family, being widespread in East-Central and Southern Europe. As with numerous other species of the genus, it is used in traditional medicine for the treatment of hepatobiliary and digestive disorders. The aim of the present study consisted of the evaluation of its alkaloid content and the assessment of its in vitro antioxidant, anti-cholinesterase and cytotoxic potential. Total alkaloid content in the composition of the species was quantified by a spectrophotometrical method and they were individually identified and quantified by HPLC-DAD. The antioxidant capacity was investigated by the DPPH and FRAP methods, while the anti-cholinesterase activity was assessed by an adapted Ellman’s method. The in vitro cytotoxic activity was evaluated on BJ human fibroblasts and DLD-1 human colon adenocarcinoma cell lines. Results showed the presence of bicuculline, protopine, chelidonine, stylopine and sanguinarine, among which bicuculline, protopine, stylopine and sanguinarine were quantified, while the antioxidant and anti-cholinesterase assays showed valuable potentials. No cytotoxic effect was observed on BJ cell lines and selective cytotoxicity was expressed towards tumoral cells. In this context, F. schleicheri appears as an important medicinal species with significant potential of substitution with the officinal species.
Accelerated Genetic Gains in Early-Maturing Maize Hybrids following Three Periods of Genetic Enhancement for Grain Yield under Low and High Soil-Nitrogen Environments
Authors: Badu-Apraku Baffour , Fakorede Morakinyo Abiodun Bamidele , Abubakar Adamu Masari
Published: May 2022

Maize (Zea mays L.) is an important staple, as well as cash crop, in sub-Saharan Africa (SSA). However, its production is severely constrained by low soil nitrogen (low N). Fifty-four early-maturing hybrids developed during three breeding periods, (2008–2010, 2011–2013 and 2014–2016) were evaluated under low (30 kg ha−1) and high (120 kg ha−1) soil nitrogen (N) in Ile-Ife and Mokwa, Nigeria, from 2017 to 2019. The study was designed to (i) determine the genetic gains in grain yield of the early-maturing maize hybrids developed during the three breeding periods, (ii) determine the relationship between grain yield and other agronomic traits and (iii) identify the highest-yielding and most stable hybrids under low- and high-N environments. The 54 hybrids were evaluated using a 6 × 9 lattice design with three replications. Mean squares for hybrids were significant for measured traits under low- and high-N environments, except the mean squares for stalk lodging and EPP under low N. Annual genetic gains in grain yield were 75 kg ha−1 year−1 (2.91%) and 55 kg ha−1 year−1 (1.33%) under low- and high-N environments, respectively, indicating that substantial gains were achieved in the genetic enhancement of the early-maturing hybrids. The hybrids TZdEI 314 × TZdEI 105, TZdEI 378 × TZdEI 173, ENT 12 × TZEI 48 and TZdEI 352 × TZdEI 315 were identified as the highest-yielding and most stable across test environments and should be tested extensively on farms and commercialized in SSA.
Glycine Betaine and β-Aminobutyric Acid Mitigate the Detrimental Effects of Heat Stress on Chinese Cabbage (Brassica rapa L. ssp. pekinensis) Seedlings with Improved Photosynthetic Performance and Antioxidant System
Authors: Quan Jin , Zheng Weiwei , Wu Meifang , Shen Zhuojun , Tan Jingru , Li Zewei , Zhu Biao , Hong Seung-Beom , Zhao Yanting , Zhu Zhujun , Zang Yunxiang
Published: May 2022

Heat stress is one of the major abiotic factors that limit the growth, development, and productivity of plants. Both glycine betaine (GB) and β-aminobutyric acid (BABA) have received considerable attention due to their roles in stimulating tolerance to diverse abiotic stresses. In order to understand how GB and BABA biostimulants alleviate heat stress in a cool-weather Chinese cabbage (Brassica rapa L. ssp. pekinensis) plant, we investigated the GB- and BABA-primed heat-stressed plants in terms of their morpho-physiological and biochemical traits. Priming with GB (15 mM) and BABA (0.2 mM) was conducted at the third leaf stage by applying foliar sprays daily for 5 days before 5 days of heat stress (45 °C in 16 h light/35 °C in 8 h dark) on Chinese cabbage seedlings. The results indicate that GB and BABA significantly increased chlorophyll content, and the parameters of both gas exchange and chlorophyll fluorescence, of Chinese cabbage under heat stress. Compared with the unprimed heat-stressed control, the dry weights of GB- and BABA-primed plants were significantly increased by 36.36% and 45.45%, respectively. GB and BABA priming also greatly mitigated membrane damage, as indicated by the reduction in malondialdehyde (MDA) and electrolyte leakage through the elevation of proline content, and increased activity levels of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX). Taken together, GB and BABA have great potential to enhance the thermotolerance of Chinese cabbage through higher photosynthesis performance, osmoprotection, and antioxidant enzyme activity.
Chemometric Analysis Based on GC-MS Chemical Profiles of Three Stachys Species from Uzbekistan and Their Biological Activity
Authors: Gad Haidy A. , Mukhammadiev Elbek A. , Zengen Gokhan , Musayeib Nawal M. Al , Hussain Hidayat , Bin Ware Ismail , Ashour Mohamed L. , Mamadalieva Nilufar Z.
Published: May 2022

The chemical composition of the essential oils (EOs) of Stachys byzantina, S. hissarica and S. betoniciflora growing in Uzbekistan were determined, and their antioxidant and enzyme inhibitory activity were assessed. A gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of 143 metabolites accounting for 70.34, 76.78 and 88.63% of the total identified components of S. byzantina, S. hissarica and S. betoniciflora, respectively. Octadecanal (9.37%) was the most predominant in S. betoniciflora. However, n-butyl octadecenoate (4.92%) was the major volatile in S. byzantina. Benzaldehyde (5.01%) was present at a higher percentage in S. hissarica. A chemometric analysis revealed the ability of volatile profiling to discriminate between the studied Stachys species. The principal component analysis plot displayed a clear diversity of Stachys species where the octadecanal and benzaldehyde were the main discriminating markers. The antioxidant activity was evaluated in vitro using 2,2-diphenyl-1-picryl-hydrazyl (DPPH), 2,2-azino bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), cupric reducing antioxidant capacity (CUPRAC), ferric reducing power (FRAP), chelating and phosphomolybdenum (PBD). Moreover, the ability of the essential oils to inhibit both acetyl/butyrylcholinesterases (AChE and BChE), α-amylase, α-glucosidase and tyrosinase was assessed. The volatiles from S. hissarica exhibited the highest activity in both the ABTS (226.48 ± 1.75 mg Trolox equivalent (TE)/g oil) and FRAP (109.55 ± 3.24 mg TE/g oil) assays. However, S. betoniciflora displayed the strongest activity in the other assays (174.94 ± 0.20 mg TE/g oil for CUPRAC, 60.11 ± 0.36 mg EDTA equivalent (EDTAE)/g oil for chelating and 28.24 ± 1.00 (mmol TE/g oil) for PBD. Regarding the enzyme inhibitory activity, S. byzantina demonstrated the strongest AChE (5.64 ± 0.04 mg galantamine equivalent (GALAE)/g oil) and tyrosinase inhibitory (101.07 ± 0.60 mg kojic acid equivalent (KAE)/g) activity. The highest activity for BChE (11.18 ± 0.19 mg GALAE/g oil), amylase inhibition (0.76 ± 0.02 mmol acarbose equivalent (ACAE)/g oil) and glucosidase inhibition (24.11 ± 0.06 mmol ACAE/g oil) was observed in S. betoniciflora. These results showed that EOs of Stachys species could be used as antioxidant, hypoglycemic and skincare agents.
Effects of Chilling Stress on Morphological, Physiological, and Biochemical Attributes of Silage Corn Genotypes during Seedling Establishment
Authors: Wu Jiaxu , Nadeem Muhammad , Galagedara Lakshman , Thomas Raymond , Cheema Mumtaz
Published: May 2022

Chilling stress is one of the major abiotic stresses which hinder seedling emergence and growth. Herein, we investigated the effects of chilling/low temperature stress on the morphological, physiological, and biochemical attributes of two silage corn genotypes during the seedling establishment phase. The experiment was conducted in a growth chamber, and silage corn seedlings of Yukon-R and A4177G-RIB were grown at optimum temperature up to V3 stage and then subjected to five temperature regimes (25 °C as control, 20 °C, 15 °C, 10 °C, and 5 °C) for 5 days. After the temperature treatment, the morphological, physiological, and biochemical parameters were recorded. Results indicated that temperatures of 15 °C and lower significantly affected seedling growth, photosynthesis system, reactive oxygen species (ROS) accumulation, and antioxidant enzyme activities. Changes in seedlings’ growth parameters were in the order of 25 °C > 20 °C > 15 °C > 10 °C > 5 °C, irrespective of genotypes. The chlorophyll content, photosynthetic rate, and maximal photochemical efficiency of PS-II (Fv/Fm) were drastically decreased under chilling conditions. Moreover, chilling stress induced accumulation of hydrogen peroxide (H2O2)and malonaldehyde (MDA) contents. Increased proline content and enzymatic antioxidants, including superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxide (APX), were found to alleviate oxidative damage under chilling stress. However, the genotype of Yukon-R exhibited better adaption to chilling stress than A4177G3-RIB. Yukon-R showed significantly higher proline content and enzymatic antioxidant activities than A4177G3-RIB under severe chilling conditions (temperature ≤ 10 °C). Similarly, Yukon-R expressed low temperature-induced ROS accumulation. Furthermore, the interaction effects were found between temperature treatment and genotype on the ROS accumulation, proline content and antioxidant enzyme activities. In summary, the present study indicated that Yukon-R has shown better adaptation and resilience against chilling temperature stress, and therefore could be considered a potential candidate genotype to be grown in the boreal climate.
Anti-Inflammatory and Pro-Regenerative Effects of Hyaluronan-Chitlac Mixture in Human Dermal Fibroblasts: A Skin Ageing Perspective
Authors: Donato Alice , Belluzzi Elisa , Mattiuzzo Elena , Venerando Rina , Cadamuro Massimiliano , Ruggieri Pietro , Vindigni Vincenzo , Brun Paola
Published: May 2022

Inflammation and the accumulation of reactive oxygen species (ROS) play an important role in the structural and functional modifications leading to skin ageing. The reduction of inflammation, cellular oxidation and dermal extracellular matrix (ECM) alterations may prevent the ageing process. The aim of this study is to investigate the expression of pro-inflammatory markers and ECM molecules in human dermal fibroblasts derived from young and middle-aged women and the effects of lactose-modified chitosan (Chitlac®, CTL), alone or in combination with mid-MW hyaluronan (HA), using an in vitro model of inflammation. To assess the response of macrophage-induced inflamed dermal fibroblasts to HA and CTL, changes in cell viability, pro-inflammatory mediators, MMPs and ECM molecules expression and intracellular ROS generation are analysed at gene and protein levels. The expression of pro-inflammatory markers, galectins, MMP-3 and ECM molecules is age-related. CTL, HA and their combination counteracted the oxidative damage, stimulating the expression of ECM molecules, and, when added to inflamed cells, restored the baseline levels of IL-1β, TNF-α, GAL-1, GAL-3 and MMP-3. In conclusion, HA and CTL mixture attenuated the macrophage-induced inflammation, inhibited the MMP-3 expression, exhibited the anti-oxidative effects and exerted a pro-regenerative effect on ECM.
Elastomer–Hydrogel Systems: From Bio-Inspired Interfaces to Medical Applications
Authors: Demirci Gokhan , Niedźwiedź Malwina J. , Kantor-Malujdy Nina , El Fray Miroslawa
Published: May 2022

Novel advanced biomaterials have recently gained great attention, especially in minimally invasive surgical techniques. By applying sophisticated design and engineering methods, various elastomer–hydrogel systems (EHS) with outstanding performance have been developed in the last decades. These systems composed of elastomers and hydrogels are very attractive due to their high biocompatibility, injectability, controlled porosity and often antimicrobial properties. Moreover, their elastomeric properties and bioadhesiveness are making them suitable for soft tissue engineering. Herein, we present the advances in the current state-of-the-art design principles and strategies for strong interface formation inspired by nature (bio-inspiration), the diverse properties and applications of elastomer–hydrogel systems in different medical fields, in particular, in tissue engineering. The functionalities of these systems, including adhesive properties, injectability, antimicrobial properties and degradability, applicable to tissue engineering will be discussed in a context of future efforts towards the development of advanced biomaterials.
Multilayer Packaging in a Circular Economy
Authors: Schmidt Jannick , Grau Laura , Auer Maximilian , Maletz Roman , Woidasky Jörg
Published: May 2022

Sorting multilayer packaging is still a major challenge in the recycling of post-consumer plastic waste. In a 2019 Germany-wide field study with 248 participants, lightweight packaging (LWP) was randomly selected and analyzed by infrared spectrometry to identify multilayer packaging in the LWP stream. Further investigations of the multilayer packaging using infrared spectrometry and microscopy were able to determine specific multilayer characteristics such as typical layer numbers, average layer thicknesses, the polymers of the outer and inner layers, and typical multilayer structures for specific packaged goods. This dataset shows that multilayer packaging is mainly selected according to the task to be fulfilled, with practically no concern for its end-of-life recycling properties. The speed of innovation in recycling processes does not keep up with packaging material innovations.
Assessment of the Refractive Index and Extinction Coefficient of Graphene-Poly(3-hexylthiophene) Nanocomposites
Authors: Velasco Davoise Lara , Peña Capilla Rafael , Díez-Pascual Ana M.
Published: May 2022

Poly(3-hexylthiophene) (P3HT) is one of the most attractive polymeric donor materials used in organic solar cells because of its high electrical conductivity and solubility in various solvents. However, its carrier mobility is low when compared to that of inorganic semiconductors; hence, the incorporation of appropriate nanomaterials to improve its electrical mobility and optical properties are pursued. In this work, a review of the changes in electrical conductivity, bandgap, hole collection properties and carrier mobility of P3HT when adding graphene (G) is presented. The main aim is to assess how the addition of different G contents influences the optical constants: refractive index (n) and extinction coefficient (k). The values of n and k as a function of the wavelength for six P3HT/G nanocomposites with G loadings in the range of 0.1–5 wt% have been fitted to two different models, Forouhi Bloomer and Cauchy, showing very good agreement between the experimental and the theoretical values. Furthermore, a rule of mixtures was successfully applied to calculate n using mass fraction instead of volume fraction, with errors lower than 6% for all the nanocomposites studied.
Influence of Elevated Temperature on the Mechanical Properties of Hybrid Flax-Fiber-Epoxy Composites Incorporating Graphene
Authors: Oun Amer , Manalo Allan , Alajarmeh Omar , Abousnina Rajab , Gerdes Andreas
Published: May 2022

Natural fibers are now becoming widely adopted as reinforcements for polymer matrices to produce biodegradable and renewable composites. These natural composites have mechanical properties acceptable for use in many industrial and structural applications under ambient temperatures. However, there is still limited understanding regarding the mechanical performance of natural fiber composites when exposed to in-service elevated temperatures. Moreover, nanoparticle additives are widely utilized in reinforced composites as they can enhance mechanical, thermal, and physical performance. Therefore, this research extensively investigates the interlaminar shear strength (ILSS) and flexural properties of flax fiber composites with graphene at different weight percentages (0%, 0.5%, 1%, and 1.5%) and exposed to in-service elevated temperatures (20, 40, 60, 80, and 100 °C). Mechanical tests were conducted followed by microscopic observations to analyze the interphase between the flax fibers and epoxy resin. The results showed that a significant improvement in flexural strength, modulus, and interlaminar shear strength of the composites was achieved by adding 0.5% of graphene. Increasing the graphene to 1.0% and 1.5% gradually decreased the enhancement in the flexural and ILSS strength. SEM observations showed that voids caused by filler agglomeration were increasingly formed in the natural fiber reinforced composites with the increase in graphene addition.
Analysis of the Warpage Phenomenon of Micro-Sized Parts with Precision Injection Molding by Experiment, Numerical Simulation, and Grey Theory
Authors: Lin Wei-Chun , Fan Fang-Yu , Huang Chiung-Fang , Shen Yung-Kang , Wang Hao
Published: May 2022

In this study, we determined the effects of design and processing parameters of precision injection molding (PIM) to minimize warpage phenomena of micro-sized parts using various plastics (polyoxymethylene (POM), acrylonitrile-butadiene-styrene (ABS), polypropylene (PP), polyamide (PA), and ABS+ polycarbonate (PC)). We applied a numerical simulation (Moldflow) to determine the runner’s balance in multi-cavities of the micro-sized part and simulate the warpage phenomenon of micro-parts with PIM. We used simulation data to fabricate a steel mold by computer numerical control (CNC) machining. In this, we study manufactured a micro-sized part and measured its warpage value using various PIM process parameters (melt temperature, mold temperature, injection pressure, and filling time). In order to obtain optimal results (i.e., minimum warpage), we employed the Taguchi method and grey theory to discern the influence of each process parameter on PIM. Finally, we determined that the most significant PIM process parameter influencing the warpage phenomenon of micro-sized parts was the mold temperature, regardless of whether in terms of the experimental results, numerical simulations, or grey theory. The PA material had the most suitable properties for application for micro-sized parts, regardless of whether in terms of experimental results, numerical simulations, or grey theory for PIM. This study also illustrates that micro-sized parts can be fabricated by PIM without the use of micro-injection molding, and we determined that the mold temperature required for molding does not need to be higher than the glass-transition temperature of the material.
Water-Triggered Self-Healing Composite Coating: Fabrication and Anti-Corrosion Application
Authors: Hao Zhentao , Chen Si , Chen Zhiwei , Lin Zhifeng , Li Weihua
Published: May 2022

Self-healing coatings formulated by stimuli-responsive container technology are regarded as a prospective strategy for long-term corrosion protection. However, such types of coatings suffer from low coating adaptability and delays in corrosion protection because the occurrence of corrosion is prior to the release of healants from containers. Herein, we took advantage of the easy hydrolysis of MOF-199 for water-induced self-healing properties. Mixed corrosion inhibitors were loaded into MOF-199 and then incorporated into acrylic coating. The water sensitivity of MOF-199 was investigated and EIS tests were used to evaluate the self-healing performance. Due to the collapse of the porous MOF-199 structure, corrosion inhibitors could be released from MOF-199 with the invasion of water into acrylic coating. The corrosion resistance performance of damaged self-healing coating gradually increased. The metal exposed to artificial defects was well protected due to a barrier formed by corrosion inhibitors. Owing to these merits, this self-healing coating is recommended for use in various fields of engineering for corrosion resistance.
Influence of Ionomer and Cyanuric Acid on Antistatic, Mechanical, Thermal, and Rheological Properties of Extruded Carbon Nanotube (CNT)/Polyoxymethylene (POM) Nanocomposites
Authors: Yun Sang-Seok , Shin Dong-hyeok , Jang Keon-Soo
Published: May 2022

The electrical properties of carbon-based filler-embedded polymer nanocomposites are essential for various applications such as antistatic and electromagnetic interference (EMI) applications. In this study, the impact of additives (i.e., ethylene-co-acid-co-sodium acid copolymer-based ionomer and cyanuric acid) on the antistatic, mechanical, thermal, and rheological properties of extruded multiwalled carbon nanotube (MWCNT)/polyoxymethylene (POM) nanocomposites were systematically investigated. The effects of each additive and the combination of additives were examined. Despite a slight reduction in mechanical properties, the incorporation of ionomer (coating on CNTs) and/or cyanuric acid (π-π interaction between CNTs and cyanuric acid) into the POM/CNT nanocomposites improved the CNT dispersity in the POM matrix, thereby enhancing electrical properties such as the electrical conductivity (and surface resistance) and electrical conductivity monodispersity. The optimum composition for the highest electrical properties was determined to be POM/1.5 wt% CNT/3.0 wt% ionomer/0.5 wt% cyanuric acid. The nanocomposites with tunable electrical properties are sought after, especially for antistatic and EMI applications such as electronic device-fixing jigs.
Enzymatic Degradation of the Most Common Aliphatic Bio-Polyesters and Evaluation of the Mechanisms Involved: An Extended Study
Authors: Rosato Antonella , Romano Angela , Totaro Grazia , Celli Annamaria , Fava Fabio , Zanaroli Giulio , Sisti Laura
Published: May 2022

Commercial hydrolytic enzymes belonging to different subclasses (several lipases, proteinase k, cutinase) were investigated for their ability to degrade different aliphatic polyesters, i.e., poly(butylene succinate) (PBS), poly(butylene succinate-co-adipate) (PBSA), two poly(caprolactone), having two different molecular weights, poly(lactic acid) (PLA) and poly(propylene carbonate) (PPC). The enzyme screening was first carried out by investigating the capacity of fully degrading the target polymers in 24 h, then weight loss measurements of selected polyesters and target enzymes were performed. Solid residues after enzyme degradation were characterized by proton nuclear magnetic resonance (1H NMR), gel permeation chromatography (GPC), infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC) and thermogravimetry (TGA). Liquid fractions were studied via GPC, 1H NMR and high-performance liquid chromatography (HPLC). PCL and PBSA were found to be the most biodegradable polyesters, under the conditions used in this study. PBS was fully degraded only by cutinase, whereas none of the tested enzymes were able to completely degrade PLA and PPC, in the conditions assessed here. Cutinase exhibited the highest hydrolytic activity on PBSA, while lipase from Candida sp. (CALB) on low molecular weight PCL. Chemical analyses on residual solids showed that the enzymatic degradation occurred homogeneously from the surface through an erosion mechanism and did not significantly affect the macromolecular structure and thermal stability. Cleaving action mode for each enzyme (endo- and/or exo-type) on the different polyesters were also proposed based on the evaluation of the degradation products in the liquid fraction.
Effect of Stability of Two-Dimensional (2D) Aminoethyl Methacrylate Perovskite Using Lead-Based Materials for Ammonia Gas Sensor Application
Authors: Mat Yunin Muhamad Yuzaini Azrai , Mohd Adenam Norfatihah , Khairul Wan M. , Yusoff Abdul Hafidz , Adli Hasyiya Karimah
Published: May 2022

Changes in physical properties of (H2C=C(CH3)CO2CH2CH2NH3)2PbI2Cl2 and (H2C=C(CH3)CO2CH2CH2NH3)2Pb(NO3)2Cl2 (2D) perovskite materials from iodide-based (I-AMP) and nitrate-based (N-AMP) leads were investigated at different durations (days) for various storage conditions. UV-Vis spectra of both samples showed an absorption band of around λmax 420 nm due to the transition of n to π* of ethylene (C=C) and amine (NH2). XRD perovskite peaks could be observed at approximately 25.35° (I-AMP) and 23.1° (N-AMP). However, a major shift in I-AMP and dramatic changes in the crystallite size, FHWM and crystallinity percentage highlighted the instability of the iodide-based material. In contrast, N-AMP showed superior stability with 96.76% crystallinity even at D20 under the S condition. Both materials were exposed to ammonia (NH3) gas, and a new XRD peak of ammonium lead iodide (NH4PbI3) with a red-shifted perovskite peak (101) was observed for the case of I-AMP. Based on the FWHM, crystallite size, crystallinity and lattice strain analysis, it can be concluded N-AMP’s stability was maintained even after a few days of exposure to the said gases. These novel nitrate-based lead perovskite materials exhibited great potential for stable perovskite 2D materials and recorded less toxicity compared to famous lead iodide (PbI2) material.
Effect of PMMA Coupling Layer in Enhancing the Ultrasonic Weld Strength of Novel Room Temperature Curable Acrylic Thermoplastic to Epoxy Based Composites
Authors: Gohel Goram , Soh Chun Zhi , Leong Kah Fai , Gerard Pierre , Bhudolia Somen K.
Published: May 2022

The joining of composites can be performed in an extremely short time with more energy-efficient ultrasonic welding techniques. The current research investigated the performance optimization of ultrasonic welding of carbon/Elium® composite to carbon/epoxy composite using a polymethyl methacrylate (PMMA) coupling interlayer. The weld strength was quantified by static lap shear strength (LSS) testing. A new methodology was used by creating a PMMA coupling layer on the epoxy composite adherend to achieve an improved interphase and thus enhance the weld properties. The LSS of Elium (EL)-Epoxy (EP) _0.25_0.25 was found to be 190% higher compared to that of EL-EP, confirming the effectiveness of the strategy used for creating an interlayer thermoplastic coupling layer. The time required for welding was optimized to be 2s as compared to 10 min required for adhesive bonding. Scanning electron microscopic images of epoxy and PMMA/Elium matrix interphase were observed to have a rough surface and remained largely unaffected by welding. There was an interphase change further away from the interphase to a rougher texture. There was little to no effect on the penultimate layer on the weld strength, as no interphase change could be observed after welding. Fractography investigation revealed shear cusps, matrix plastic deformation, fiber imprints, fiber pull-out, and good adhesion between matrix and fiber, features seen for configuration with maximum LSS. The current research findings present a way to join Elium® with epoxy composites that could be used in applications that require a selective strengthening, such as in sporting goods and consumer products. Furthermore, a detailed investigation is ongoing to use different filler particles and coupling layers to reach the maximum welding performance.
Aquaponics-Derived Tilapia Skin Collagen for Biomaterials Development
Authors: Gallo Nunzia , Natali Maria Lucia , Quarta Alessandra , Gaballo Antonio , Terzi Alberta , Sibillano Teresa , Giannini Cinzia , De Benedetto Giuseppe Egidio , Lunetti Paola , Capobianco Loredana , Blasi Federica Stella , Sicuro Alessandro , Corallo Angelo , Sannino Alessandro , Salvatore Luca
Published: May 2022

Collagen is one of the most widely used biomaterials in health-related sectors. The industrial production of collagen mostly relies on its extraction from mammals, but several issues limited its use. In the last two decades, marine organisms attracted interest as safe, abundant, and alternative source for collagen extraction. In particular, the possibility to valorize the huge quantity of fish industry waste and byproducts as collagen source reinforced perception of fish collagen as eco-friendlier and particularly attractive in terms of profitability and cost-effectiveness. Especially fish byproducts from eco-sustainable aquaponics production allow for fish biomass with additional added value and controlled properties over time. Among fish species, Oreochromis niloticus is one of the most widely bred fish in large-scale aquaculture and aquaponics systems. In this work, type I collagen was extracted from aquaponics-raised Tilapia skin and characterized from a chemical, physical, mechanical, and biological point of view in comparison with a commercially available analog. Performed analysis confirmed that the proprietary process optimized for type I collagen extraction allowed to isolate pure native collagen and to preserve its native conformational structure. Preliminary cellular studies performed with mouse fibroblasts indicated its optimal biocompatibility. All data confirmed the eligibility of the extracted Tilapia-derived native type I collagen as a biomaterial for healthcare applications.
Evaluation of Inhibitory Activities of Sophora flavescens and Angelica gigas Nakai Root Extracts against Monoamine Oxidases, Cholinesterases, and β-Secretase
Authors: Park Jong Eun , Mun Seul-Ki , Yee Sung-Tae , Kim Hoon
Published: May 2022

In this study, Sophora flavescens (SF) from Yeongcheon (YSF) and Mt. Jiri (JiSF), and Angelica gias (AG) from Yeongcheon (YAG), Mt. Jiri (JiAG), and Jecheon (JeAG) were extracted using three concentrations of ethanol, 95% (95Et), 70% (70Et), and 50% (50Et), and hot water (DW) to evaluate the inhibitions of monoamine oxidases (MAOs; MAO-A and B), cholinesterases (ChEs; AChE and BChE) and β-secretase (BACE1) for targeting depression and neurodegenerative diseases. There were no significant differences in constituent compounds depending on herbal origins, except that YSF-95Et and JiSF-95Et showed a distinct non-polar spot upper maackiain position, and JiAG and JeAG showed a higher amount of decursin than YAG. Ethanolic YAG and JeAG extracts showed the highest MAO-A inhibition, and YSF-95Et mostly inhibited MAO-B. JiSF-95Et showed the highest AChE inhibition and YSF-70Et, JiSF-95Et, and -70Et showed the highest BChE inhibition. Interestingly, ethanolic AG extracts showed extremely potent BACE1 inhibition, especially for JiAG-95Et and JeAG-50Et, whereas there have been no reports about BACE1 inhibition of decursin, the major compound, or AG extracts in other studies. All extracts were nontoxic to MDCK and SH-SY5Y with a low toxicity to HL-60. The results showed a different pattern of inhibitory activities of the extracts toward target enzymes depending on the origins, and multi-target abilities, especially for MAO-B and BChE by YSF-95Et, for AChE and BChE by JiSF-95Et, and for MAO-B and BACE1 by JiAG-95Et. It is suggested that those extracts are potential candidates for finding novel compounds with multi-target inhibitory activities, and herbal origin is an important factor to be considered in selection of the plants.
Polyethylenimine/cGAMP Nanocomplexes for STING-Mediated Cancer Immunotherapy: Formulation and Characterization Using Orthogonal Techniques
Authors: Petrovic Marija , Borchard Gerrit , Jordan Olivier
Published: May 2022

Cyclic GMP-AMP (cGAMP) has lately been extensively investigated in cancer immunotherapy due its activation of the innate immunity stimulation of interferon genes (STING) pathway within antigen presenting cells (APC) leading to an increase in tumor specific CD8+ T cells. As negatively charged dinucleotides are prone to enzymatic degradation before being taken up by APC, there is a need for an appropriate carrier. Therefore, polyethylenimine (PEI), a gold standard for oligonucleotide delivery, was selected. Molecular weight, type of PEI and N/P ratio between PEI/cGAMP were investigated in terms of toxicity, efficacy and physicochemical properties of the nanocomplexes (NCs) such as size, zeta potential and shape. Due to lack of nano-medicine regulations and the need for a case-by case assessment, here we examine these parameters by several orthogonal methods, such as dynamic light scattering (DLS), nanoparticle tracking analysis (NTA) and online asymmetric flow field flow fractionation (AF4) connected to DLS. N/P ratio of 2/1 ratio using linear PEI 25 kDa resulted in larger, positively charged particles of elongated shape, which were shown to have the best toxicity/efficacy ratio among different PEIs and ratios tested.
Integrating Triple Bottom Line in Sustainable Chemical Supplier Selection: A Compromise Decision-Making-Based Spherical Fuzzy Approach
Authors: Wang Chia-Nan , Chou Chien-Chang , Dang Thanh-Tuan , Nguyen Hoang-Phu , Nguyen Ngoc-Ai-Thy
Published: May 2022

As a consequence of increased awareness of environmental preservation and the associated rigorous regulations, the adoption of sustainable practices has become a crucial element for corporate organizations in regard to their supply chains. In the chemical industry, which is characterized by high risks, high pollution, and high efficiency, these characteristics can help businesses analyze their long-term development and sustainability. The goal of this research is to analyze and choose possible suppliers based on their sustainability performance in the chemical sector. A methodology based on multi-criteria decision making (MCDM) is proposed for this evaluation, using spherical fuzzy analytical hierarchy process (SF-AHP) and combined compromise solution (CoCoSo) methods, in which the novel spherical fuzzy sets theory is employed to present the ambiguous linguistic preferences of experts. In the first stage, an evaluation criteria system is identified through literature review and experts’ opinions. The SF-AHP is used to determine the criteria weights, while the CoCoSo method is utilized to select the right sustainable supplier. A case study in the chemical industry in Vietnam is presented to demonstrate the effectiveness of the proposed approach. From the SF-AHP findings, “equipment system and technology capability”, “flexibility and reliability”, “logistics cost”, “green materials and technologies”, and “on-time delivery” were ranked as the five most important criteria. From the CoCoSo analysis, Vietnam National Chemical Group (CHE-05) was found to be the best supplier. A sensitivity study and a comparison analysis of methods were also conducted to verify the robustness of the proposed model, and the priority rankings of the best suppliers were very similar. To the best of our knowledge, this is the first study that has proposed SF-AHP and CoCoSo to prioritize SSS evaluation criteria and determine the best alternatives. The suggested method and findings can be used to make well-informed decisions that help businesses to achieve supply chain sustainability, capture opportunities, and maintain competitiveness through reconfiguring resources. The method could be useful for case studies in other countries and for other sustainability problems.
Insights on Proteomics-Driven Body Fluid-Based Biomarkers of Cervical Cancer
Authors: Mukherjee Amrita , Pednekar Chinmayi Bhagwan , Kolke Siddhant Sujit , Kattimani Megha , Duraisamy Subhiksha , Burli Ananya Raghu , Gupta Sudeep , Srivastava Sanjeeva
Published: May 2022

Cervical cancer is one of the top malignancies in women around the globe, which still holds its place despite being preventable at early stages. Gynecological conditions, even maladies like cervical cancer, still experience scrutiny from society owing to prevalent taboo and invasive screening methods, especially in developing economies. Additionally, current diagnoses lack specificity and sensitivity, which prolong diagnosis until it is too late. Advances in omics-based technologies aid in discovering differential multi-omics profiles between healthy individuals and cancer patients, which could be utilized for the discovery of body fluid-based biomarkers. Body fluids are a promising potential alternative for early disease detection and counteracting the problems of invasiveness while also serving as a pool of potential biomarkers. In this review, we will provide details of the body fluids-based biomarkers that have been reported in cervical cancer. Here, we have presented our perspective on proteomics for global biomarker discovery by addressing several pertinent problems, including the challenges that are confronted in cervical cancer. Further, we also used bioinformatic methods to undertake a meta-analysis of significantly up-regulated biomolecular profiles in CVF from cervical cancer patients. Our analysis deciphered alterations in the biological pathways in CVF such as immune response, glycolytic processes, regulation of cell death, regulation of structural size, protein polymerization disease, and other pathways that can cumulatively contribute to cervical cancer malignancy. We believe, more extensive research on such biomarkers, will speed up the road to early identification and prevention of cervical cancer in the near future.
Are Ashes All That Is Left? Grace Jantzen’s Aesthetics and the Beauty of Biodiversity
Authors: Dean Dorothy C.
Published: May 2022

As the climate crisis continues to worsen and it becomes apparent that the earth faces its sixth mass extinction event, it is more important than ever to find an alternative to the disordered thinking that prevents meaningful environmental reform in nations of the Global North with large carbon footprints such as the United States. Informed by affect theory, I revisit Grace Jantzen’s late work on death and beauty in the context of biodiversity to develop the beginnings of a theological affect of responsiveness to ecological beauty. Juxtaposing Jantzen’s theory of the displacement of beauty with Kevin O’Brien’s theological ethics of biodiversity, I suggest that biodiversity can be key to an ecotheology that combats human exceptionalism and prioritizes responsiveness to beauty. I contend that an aesthetics of natality requires responsiveness to the beauty of biodiversity in order to combat both human exceptionalism and the culture of necrophilia that Jantzen critiqued. Ultimately, I conclude that beauty, natality, and biodiversity may be able to inform an ecological theology centered on nonexceptional theological affects.
Finding Rhythm for Multicultural Worship: Heartful Indwelling with God and God’s Creation
Authors: Seonwoo Hyuk
Published: May 2022

Justice seeking is an indispensable component of multicultural worship. How can multicultural worship help the faith community worship God heartfully, indwelling with God, one another, and creation? In this article, the Tai Chi rhythm of “loosen-empty-push” will be employed as both a metaphoric and embodied way of integration and mutual indwelling in heartful worship. For example, in the rhythm of multicultural worship, we humbly recognize our brokenness and vulnerability while being receptive to God’s initiative love and grace (loosen). We continue to learn how to let go and surrender ourselves to the works of the Holy Spirit, also becoming part of a multicultural and mutually embracing body of Christ, whose entire life embodied God’s reconciling and self-giving love (empty). One of the characteristics of multicultural worship is solidarity with others including God’s creation through worshipers’ daily compassionate, justice-seeking lives (push).
Initial In-Flight Spectral Calibration of the Near-Infrared Spectra Acquired by the MarSCoDe Onboard the Zhurong Rover
Authors: Wu Bing , Liu Chengyu , Xu Rui , Lin Honglei , Xu Xuesen , Yan Wei , Tan Yongjian , Liu Bin , Ren Xin , Xu Weiming , Liu Xiangfeng , Zhang Zhenqiang , Yang Benyong , He Zhiping , Shu Rong
Published: May 2022

The Zhurong rover successfully landed in southern Utopia Planitia as part of the Tianwen-1 mission on 15 May 2021. One of the objectives of the Mars Surface Composition Detector (MarSCoDe) onboard the rover is to investigate mineral compositions on the Martian surface by measuring the near-infrared reflectance spectra. Before conducting spectral interpretation, in-flight data calibration is a crucial step due to the significant differences between the laboratory and the Martian environment. The work of the MarSCoDe spectrometer is based on an acousto-optic tunable filter (AOTF). The temperature variation of the AOTF could induce wavelength offset, making mineral identification uncertain. We first analyzed the viewing geometry of the spectral measurements of the calibration targets on the Mars rover according to its attitude to identify the anomalous data. The wavelength offsets were then determined by fitting the absorption positions of CO2 at ~1400 and ~2000 nm, representing the primary composition of the Martian atmosphere. The results showed 2–8 nm wavelength offsets, which correlated well with AOTF temperatures. The artifacts were removed in the wavelength-corrected reflectance spectra, which is critical to identify the material types on Mars, especially water-related minerals.
Spatial and Seasonal Variations of the Island Mass Effect at the Sub-Antarctic Prince Edward Islands Archipelago
Authors: Lamont Tarron , Toolsee Tesha
Published: May 2022

At the sub-Antarctic Prince Edward Islands (PEIs) in the Southern Ocean, the Island Mass Effect (IME) plays an important role in maintaining an ecosystem able to support diverse biological communities; however, limited in situ sampling has severely constrained our understanding of it. As such, our study used satellite chlorophyll a (chla) to provide the first detailed characterisation of the spatial extent and seasonal variability of the IME at the PEIs. Seasonal surface chla variations were remarkable, with localised increases observed from mid-austral spring to the end of autumn (October to May). In contrast, during June to September, there were no distinguishable differences between chla at the PEIs and that further afield. Seasonal chla changes were significantly correlated with higher light levels, warmer waters, and shallow upper mixed layer depths reflecting enhanced water column stability during summer and autumn, with the opposite pattern in winter and spring. The IME extended northeast of the islands and remained spatially distinct from elevated chla around the northern branch of the sub-Antarctic Front and the southern branch of the Antarctic Polar Front. From December to February, the IME was spatially connected to the island shelf. In contrast, during March–May and in October, higher chla was observed only to the northeast, some distance away from the islands, suggesting a delayed IME, which has not previously been observed at the PEIs. The clear association of this higher chla with the weak mean geostrophic circulation northeast of the islands suggested retention and accumulation of nutrients and phytoplankton biomass, which was likely aided by wind-driven northeastward transport of water from the shelf. Climatological mean chla to the northeast was generally higher than that on the PEI shelf, and further research is required to determine the importance of this region to ecosystem functioning at the islands.
Analysis and Correction of Antenna Pattern Effects in AMAO Spaceborne SAR Images
Authors: Huang Jianjun , Chen Jie , Guo Yanan , Wang Pengbo
Published: May 2022

Azimuthal multi-angle observation (AMAO) is a novel spaceborne synthetic aperture radar (SAR) technique proposed recently, which significantly improves the acquisition performance of target information. It has highly flexible antenna beams with powerful beam-steering capability while affecting the consistency of radiometric measurements, especially in the high-squint case. This paper mainly focuses on the antenna pattern effect analysis and correction method of AMAO spaceborne SAR. The antenna pattern characteristics of the squint steering beam are first analyzed. On this basis, an analytical model is developed to quantify the antenna pattern effects in AMAO SAR images. Different from the conventional approach that corrects the elevation antenna pattern, an improved antenna pattern correction method, which accounts for the spatial variation in the two-dimensional antenna pattern, is then proposed for AMAO spaceborne SAR. Finally, experiments are conducted to verify the proposed correction method. The results show that the newly proposed method considering the spatial-variant antenna pattern has higher performance than the conventional approach using a reference elevation pattern.
Aircraft and Satellite Observations of Vortex Evolution and Surface Wind Asymmetry of Concentric Eyewalls in Hurricane Irma
Authors: Hua Han , Zhang Biao , Zhang Guosheng , Perrie William , Chen Changlin , Li Yuanben
Published: May 2022

We compare the vortex evolutions of eyewall replacement cycles (ERCs) between the sea-surface and the free-atmosphere levels and investigate the asymmetric structure of concentric eyewalls (CEs) by examining a combination of aircraft observations and surface wind fields derived from C-band spaceborne synthetic aperture radar (SAR) images during Hurricane Irma (2017) from 4 September 2017 to 8 September 2017. A total of 116 radial wind profiles measured by an aircraft were collected and showed that ERCs occur at both the sea-surface and the free-atmosphere levels. The outer eyewall was shown to form at the free atmospheric level (~3 km) with a narrow structure at the sea-surface level and an outward tilt with height in the cross-section. In our study, four ERC events were determined from wind profile parameters fitted by a modified Rankine vortex model, which was validated by 328 radial legs collected from six hurricanes. The outer eyewall did not replace the inner eyewall at the sea-surface level in the third ERC, due to the maintenance of a short duration and intense original eyewall. Additionally, Irma’s intensity weakened during the fourth ERC rather than re-intensified, because of the generation of a third wind maximum outside the secondary eyewall. Comparisons of five SAR-derived surface wind fields in Irma and another two hurricane cases illustrated that the location of the secondary eyewall generation is a key point in the interpretation of anomaly intensity changes in the fourth ERC.