bluuebunny/crossref_metadata_2025 huggingface dataset:
METAMORFOSIS POSMODERN DALAM KUMPULAN CERPEN EQUILIBRIUM KARYA BRAMANTIO
Authors: Aryanto Dwi Mega
Published: June 2020

Kumpulan  Cerpen Bramantio dengan judul Equilibrium memuat permasalahan penanda dan petanda akibat adanya ketidakgrammatikalan di dalam teks. Adanya permasalahan penanda dan petanda tersebut, menimbulkan asumsi adanya makna secara tekstual. Tujuan penelitian ini adalah menjabarkan dan memaknai penanda dan petanda dalam sembilan cerita kumpulan cerpen Equilibrium karya Bramantio. Penelitian ini memanfaatkan teori semiotika Roland Barthes dengan dua tahap analisis. Pertama, analisis secara tekstual terhadap penanda dan petanda di kesembilan cerpen. Kedua, setelah mengetahui tanda dari formulasi penanda dan petanda yang terdapat dalam kesembilan cerpen, kemudian tanda tadi diformulasikan dengan korelasi tanda bagan Barthesian. Berdasarkan tahap analisis, ditemukan adanya kehadiran nilai-nilai posmodern di dalam kesembilan cerpen Equilibrium yang merujuk pada tindakan-tindakan ataupun peristiwa-peristiwa yang dialami oleh para tokoh. Analisis penanda dan petanda Barthesian telah menghasilkan bahwa setiap tokoh di dalam kumpulan cerpen Equilibrium sedang bergejolak dengan diri mereka sendiri yang sekaligus menunjukkan adanya metamorfosis penanda dan petanda untuk mencapai nilai-nilai posmodern.
SPIRITUALITAS DALAM HEKSALOGI NOVEL SUPERNOVA KARYA DEE
Authors: Rosyidah Luthfita Nur
Published: June 2020

ABSTRAKPembahasan penelitian ini akan difokuskan pada tindakan plastisitas spiritualitas dalam serial novel Supernova karya Dee dengan judul Kesatria, Putri, dan Bintang Jatuh; Akar; Petir; Partikel; Gelombang; dan Intelegensi Embun Pagi. Penelitian ini memanfaatkan teori struktur naratif Vladimir Propp, yang terdiri dari dua tahap analisis. Pertama, analisis mengenai identifikasi motif spiritualitas dalam enam novel serial Supernova secara tekstual. Kedua, hasil identifikasi tersebut menunjukkan adanya unsur yang berulang terkait dengan motif spiritualitas yang disebut sebagai fungsi. Fungsi-fungsi tersebut kemudian dirumuskan sesuai dengan urutan kemunculannya dalam setiap novel. Melalui kedua tahap analisis tersebut dapat diperoleh makna dari adanya motif spiritualitas dalam keenam novel serial Supernova. Berdasarkan tahap analisis, ditemukan konsistensi motif spiritulitas dalam keenam novel merupakan sebuah proses pencarian dalam suatu kehidupan, serta merupakan usaha pemenuhan kekosongan diri untuk menemukan makna kehidupan. Plastisitas spiritualitas dalam keenam novel lebih menitikberatkan pada posisi sains sebagai unsur pembangun kesadaran setiap individu. Kata-kata kunci: Propp, fungsi, spiritualitas, plastisitas ABSTRACTThe research will be focused on the act of spirituality plasticity in Dee's Supernova novel series titled Kesatria, Putri, dan Bintang Jatuh; Akar; Petir; Partikel; Gelombang; dan Intelegensi Embun Pagi. This research utilizes the theory of narrative structure Vladimir Propp, which consists of two stages of analysis. First, an analysis of the identification of spirituality motif in six Supernova novel series. Second, the results of the identification indicate a recurring element related to the spirituality motif called function. These functions are then formulated according to the order in which they appear in each novel. Through these two stages of analysis can be obtained the meaning of the existence of a spirituality motif in the six serial Supernova novels. Based on the analysis phase, consistency of spirituality motifs can be found in the six novels as a process searching in life, and it is an attempt to fulfill the self-emptiness to find the meaning of life. Spirituality plasticity in the six novels is more focused on the role of science as an element of building awareness of each individual. Key words: Propp, function, spirituality, plasticity
Toxicidade do nitrito para o camarão Litopenaeus vannamei cultivado em sistemas de água clara e bioflocos
Authors: MELO Fabiana Penalva de , FERREIRA Maria Gabriela Padilha , BRAGA Ítalo Felipe Mascena , CORREIA Eudes De Souza
Published: June 2020

O objetivo deste trabalho foi avaliar a toxicidade do nitrito sobre o crescimento, sobrevivência e quantidade de hemócitos totais na hemolinfa de juvenis do camarão Litopenaeus vannamei (1,60±0,06 g) submetidos a diferentes concentrações de N-nitrito e dois sistemas de cultivo. Os camarões foram distribuídos em 24 unidades experimentais (área de 0,20 m2 e volume útil de 30 L), numa densidade de 75 camarões m-2, em um delineamento experimental inteiramente casualizado, em esquema fatorial 2x2, com quatro concentrações de N-nitrito (0-Controle, 10, 20 e 40 mg N-NO2 L-1) e dois sistemas de cultivo (água clara e bioflocos). Os juvenis de L. vannamei foram alimentados com ração comercial (35% proteína bruta). As concentrações de N-NO2 influenciaram significativamente (P<0,05) o peso final (1,71 a 3,36 g), a sobrevivência (40,7 a 96,3 %) e a taxa de crescimento específico (0,15 a 2,42 % dia-1). A interação entre as concentrações de N-nitrito e os sistemas de cultivo não influenciaram significativamente (P≥0,05) a quantidade total de hemócitos.  Desta forma, é possível cultivar o camarão L. vannamei em concentrações de até 20 mg N-NO2 L-1 por um período de 30 dias sem comprometer o crescimento e a sobrevivência.
Composição das capturas na pesca de atuns e afins em cardumes associados no atlântico oeste equatorial
Authors: SILVA Guelson Batista da , HAZIN Humberto Gomes , MOURATO Bruno Leite , HAZIN Fábio Hissa Vieira , FONTELES-FILHO. Antônio Adauto
Published: June 2020

O presente trabalho teve como objetivo caracterizar a composição das capturas na pesca de atuns e afins em cardumes associados no Atlântico Oeste Equatorial, realizadas por parte da frota sediada no Município de Areia Branca, RN. Para tanto foram acompanhados 109 desembarques nos cais pesqueiros da cidade, oriundos de nove embarcações com comprimento total médio de 13,4 m, no período entre junho de 2010 e maio de 2013. Além disso, também foram realizados nove embarques no período de fevereiro de 2011 a dezembro de 2012, durante os quais foi realizada a biometria das principais espécies capturadas. Os desembarques totalizaram 691.533 kg (6.344,3 kg em média ± desvio padrão de 292,7 kg) de pescado, com predominância de albacora-bandolim e albacora-laje, além de outras espécies como o dourado, agulhão-negro, bonito-listrado, cavala-empinge, peixe-rei e ocorrências esporádicas de outros peixes, como o espadarte e o cangulo.
QUALIDADE DA CARNE MECANICAMENTE SEPARADA (CMS) E DO SURIMI OBTIDOS DE PEIXES DE BAIXO VALOR COMERCIAL
Authors: GUIMARÃES Juliana de Lima Brandão , CALIXTO Flávia Aline Andrade , KELLER Luiz Antônio de Moura , Torrezan Renata , FURTADO Ângela Aparecida Lemos , MESQUITA Eliana de Fátima Marques de
Published: June 2020

Este estudo teve como objetivo analisar a qualidade e as diferenças entre a carne mecanicamente separada (CMS) e surimi obtidos a partir de pargo (Pagrus pagrus) e o tira-vira (Percophis brasiliensis) capturados na categoria "misturaââ‚¬Â da modalidade da pesca de arrasto de Niterói, RJ. Para elaboração do surimi, as CMS de pargo e tira-vira foram submetidas í­Â  lavagem. A caracterização quí­­mica dos produtos foi determinada pelas análises da composição quí­­mica (umidade, proteí­­na,lipí­­deos e cinzas). Para as análises microbiológicas foram realizadas contagens de Estafilococos coagulase positiva, coliformes a 35 Â°C, coliformes termotolerantes e pesquisa de Salmonella sp. Os surimis de pargo e tira-vira apresentaram menores teores proteico (7,84% e 7,34%) e lipí­­dico (0,73% e 0,74%) e maior umidade (91,71% e 90,15%), respectivamente, em comparação í­Â  CMS (P<0,05). A análise microbiológica demonstrou que os produtos estavam dentro do padrão sanitário exigido pela legislação. Com base nos resultados obtidos, conclui-se que os peixes de baixo valor agregado da categoria "misturaââ‚¬Â resultaram em produtos (CMS e surimi) viáveis para o uso na tecnologia do pescado. O produto CMS apresentou-se mais rico nutricionalmente do que o surimi, porém com maior carga bacteriana.
LARVICULTURA DE MOLUSCO DE AREIA CULTIVADO EM DIFERENTES DENSIDADES
Authors: LIMA Priscilla Celes Maciel de , LAVANDER Henrique David , Da Silva Luis Otavio BRITO , GÁLVEZ Alfredo Olivera
Published: June 2020

O desenvolvimento de tecnologias para o cultivo de larvas e pós-larvas em laboratório é uma alternativa para suprir a redução dos estoques naturais de Anomalocardia brasiliana. Com o objetivo de aprimorar a tecnologia de produção de moluscos em laboratório, o presente trabalho avaliou o efeito da densidade de cultivo sobre a sobrevivência e o crescimento de larvas e pós-larvas de A. brasiliana. Foram realizados dois experimentos, no primeiro avaliou-se a densidade no cultivo de larvas véliger, testando as densidades de 2, 6 e 10 larvas mL-1 durante sete dias.No segundo, foram testadas as densidades de 60 e 120 pós-larvas cm-2, durante 38 dias. No cultivo de larvas véliger, os melhores resultados para sobrevivência e crescimento foram encontrados nas densidades de 2 e 6 larvas mL-1. Quando avaliada a densidade no cultivo de pós-larvas, os melhores resultados, tanto para a sobrevivência quanto para o comprimento final, foram obtidos na densidade de 60 larvas cm-2, com 93,81 Â± 0,68% e 2,18Â± 0,18 mm, respectivamente. Dessa forma, para otimizar a produtividade, recomenda-se as densidades de 6 larvas mL-1 (larvas véliger) e de 60 pós-larvas cm-2 para o cultivo de A. brasiliana em laboratório.
DESEMPENHO PRODUTIVO DE JUVENIS DE TAMBAQUI EM TANQUE-REDE, SOB DIFERENTES TAXAS DE ALIMENTAÇÃO
Authors: MACHADO Jefferson José , PORTO Marlos Oliveira , CAVALI Jucilene , NUNES Natalia Netos dos Santos , FERREIRA Elvino , ALMEIDA Aline Ribeiro
Published: June 2020

O estudo foi desenvolvido na Fundação Universidade Federal de Rondônia, na Base de Piscicultura Carlos Eduardo Matiaze, situada no municí­­pio de Presidente Médici/RO com o objetivo de avaliar o desempenho produtivo de juvenis de tambaqui alimentados com diferentes taxas de alimentação em relação ao peso corporal (PC), cultivados em tanquesí rede. Foram utilizados 180 juvenis, com peso médio inicial de 0,033 Kg Â± 0,001 Kg distribuí­­dos em 20 tanques í  rede de 1 m3 dispostos em viveiro de 1000 m2 (20 m x 50 m) e profundidade de 1,64 m. O delineamento experimental foi ao acaso com cinco tratamentos com diferentes taxas de alimentação (TA) e quatro repetições (2, 4, 6, 8 e 10% do PC). Os parí­¢metros avaliados foram comprimento do corpo (m), comprimento da cabeça (m), altura do corpo (m), PC (Kg) e conversão alimentar. A avaliação sensorial do "baby fishââ‚¬Â também foi realizada. Os resultados deste trabalho demostraram que o melhor desempenho produtivo sob as diferentes TA fornecidas diariamente foi a de 6%, nesta taxa foi obtido maior produtividade em relação ao fator tempo. Já a análise sensorial do tambaqui baby fish cultivado em tanques-rede a TA de 10% foi o preferido dos consumidores pela caracterí­­stica macia e suculenta da carne.
COEFICIENTE DE DIGESTIBILIDADE APARENTE DE ALIMENTOS UTILIZADOS EM DIETAS DE TAMBAQUI
Authors: BUZOLLO Hellen , NASCIMENTO Thiago Matias Torres do , SANDRE Lidiane Cristina Gonçalves de , NEIRA Ligia Maria , JOMORI Rosângela Kiyoko , CARNEIRO Dalton José
Published: June 2020

A qualidade da dieta pode ser avaliada quantificando a habilidade dos peixes para digerir os alimentos. Portanto, este estudo teve como objetivo determinar os coeficientes de digestibilidade aparente (CDA) para proteí­­na bruta (PB), extrato etéreo (EE) e energia bruta (EB) de nove ingredientes proteicos e sete ingredientes energéticos mais comumente usados em dietas comerciais para tambaqui. No total, 510 juvenis de tambaqui foram alimentados com dietas-teste contendo uma dieta de referência suplementada com um marcador de digestibilidade inerte (Cr2O3). As análises de digestibilidade mostraram que o glúten de milho apresentou o CDAPB(98,09%) e CDAEB (96,91%) mais alto, enquanto que a levedura de álcool apresentou o menor CDAPB (63,17%) e CDAEB (46,24%). Os ingredientes proteicos foram revelados como excelentes fontes de lipí­­dios (CDAEE > 85,0%), com exceção do glúten de trigo (CDAEE: 63,73%). O milho (94,5%) e o farelo de trigo (86,08%) apresentaram o CDAPB mais alto de todos os ingredientes energéticos. Não foram observadas diferenças significativas no CDAEE dos ingredientes energéticos testados. Dos ingredientes energéticos testados, o óleo de milho (95,7%), óleo de peixe (93,61%), o óleo de soja (93,31%) e o milho (88,70%) apresentaram o CDAEB mais alto. O uso de ingredientes proteicos e energéticos com altos coeficientes de digestibilidade, como os relatados, pode orientar a formulação de dietas de alta qualidade para tambaqui.
The effect of intensive authentic reading tasks on reading performance of academic ielts test takers
Authors: Shobeiry Maria
Published: June 2020

This study aimed at investigating the effect of employing intensive reading (IR) authentic tasks as supplementary materials on reading comprehension development of Academic IELTS test takers within the framework of schema theory and three instructional models of reading strategies suggested by Pearson and Duke (2009). Participants of this study consist of 81 individuals comprising 41 male and 40 female language learners at pre-advanced level of English proficiency with the age range of 25 to 37 among which 42  were categorized as the experimental group which received IR treatment and the rest of 39 ,who are considered as control group, that did not receive IR treatment. The experimental group was further divided into two subgroups which each received the same IR authentic tasks with different instructional methods to teaching reading strategies.  The results of an ANCOVA analysis illustrated a significant positive influence of authentic IR treatments on the development of participants' reading comprehension skills. However, an independent one-way t-test revealed that Pearson's and Duke's (2009) various instructional methods were not significantly influential on participants' final reading proficiency development.
Technical Terms Translation: Translation Techniques and Quality of Automotive Terms in the Yamaha Nmax Owner’s Manual
Authors: Hindami Pradipta Dhaifan
Published: June 2020

In Indonesia, many types of motorcycle are produced and marketed. Most of them are made by foreign manufacturers. The manual books which are printed in foreign languages such as English may cause some problems for the motorcycle owners and users in the country. One of the problems is the translation of the technical terms in the manual books. It is thus challenging to analyze the translation techniques applied in the translation of the automotive technical terms in Yamaha Nmax Owner’s Manual, and the impact of translation techniques on the accuracy, acceptability and readability. Yamaha Nmax is one of the motorcycles with high sales rate in the country. The methods of data collection consist of content analysis and in-depth interview with the raters and respondents. The results show that there are 8 techniques used to translate the technical terms and the use of the techniques result in a translation which is in general accurate, acceptable, and readable. The technique which produces translations with high level of accuracy, acceptability and readability is established equivalent. Meanwhile, generalization tends to produce translations with low level of accuracy, while borrowing produces translations with low level of acceptability and readability.
Medications Available for Weight Reduction in Elective Total Joint Arthroplasty
Authors: Amanatullah Derek F. , Ohanisian Levonti , Bailony Rami
Published: June 2020

» Arthroplasty in patients with elevated body mass index results in increased rates of reoperation, instability, revision, and infection.

» Preoperative weight loss may reduce the complication rate associated with orthopaedic surgery.

» In addition to lifestyle modification, anti-obesity medications are available to help patients to reduce their preoperative weight.

» Currently, there are 6 U.S. Food and Drug Administration (FDA)-approved anti-obesity medications in the United States: phentermine, orlistat, phentermine with topiramate extended release (ER), lorcaserin, sustained release (SR) naltrexone with bupropion, and liraglutide.

» Anti-obesity medications potentially provide a new way to optimize patients before surgery and to ensure successful recovery postoperatively.
Stability and Behavior in Carbonate Cores for New Enhanced-Oil-Recovery Polymers at Elevated Temperatures in Hard Saline Brines
Authors: Seright Randall S. , Wavrik Kathryn E. , Zhang Guoyin , AlSofi Abdulkareem M.
Published: June 2020

The goal of this work was to identify viable polymers for use in the polymer flooding of high-temperature carbonate reservoirs with hard, saline brines. This study extensively examined recent enhanced-oil-recovery (EOR) polymers for stability, including new 2-acrylamido-tertbutylsulfonic acid (ATBS) polymers with a high degree of polymerization, scleroglucan, n-vinylpyrrolidone (NVP)-based polymers, and hydrophobic associative polymers. For each polymer, stability experiments were performed over a 2-year period under oxygen-free conditions (less than 1 ppb) at various temperatures up to 180°C in brines with total dissolved solids (TDS) ranging from 0.69 to 24.4%, including divalent cations from 0.034 to 2.16%. Use of the Arrhenius analysis was a novel feature of this work. This rarely used method allows a relatively rapid assessment of the long-term stability of EOR polymers. Rather than wait years or decades for results from conventional stability studies at the reservoir temperature, reliable estimates of the time-temperature stability relations were obtained within 2 years. Arrhenius analysis was used to project polymer-viscosity half-lives at the target reservoir temperature of 99°C. The analysis suggests that a set of ATBS polymers will exhibit a viscosity half-life over 5 years at 120°C and over 50 years at 99°C, representing a novel finding of this work and a major advance for extending polymer flooding to higher temperatures.

For five polymers that showed potential for application at higher temperatures, corefloods were performed under anaerobic conditions. Another novel feature of this work was that anaerobic floods were performed without using chemical oxygen scavengers, chemical stabilizing packages, or chelating agents (that are feared to alter rock properties). Using carbonate cores and representative conditions, corefloods were performed to evaluate polymer retention, rheology in porous media, susceptibility to mechanical degradation, and the residual resistance factor for each of the polymers at 99°C.
Transcriptome analysis provides insights into the non-methylated lignin synthesis in Paphiopedilum armeniacum seed
Authors: fang lin , Xu Xin , Li Ji , Zheng Feng , Li Mingzhi , Yan Jingwei , Li Yuan , Zhang Xinhua , Li Lin , Ma Guohua , Zhang Aying , Lv Fubin , Wu Kunlin , Zeng Songjun
Published: June 2020

Backgrounds: Paphiopedilum is an important genus of the orchid family Orchidaceae and has high horticultural value. The wild populations are under threat of extinction because of overcollection and habitat destruction. Mature seeds of most Paphiopedilum species are difficult to germinate, which severely restricts their germplasm conservation and commercial production. The factors inhibiting germination are largely unknown.Results: In this study, large amounts of non-methylated lignin accumulated during seed maturation of Paphiopedilum armeniacum (P. armeniacum), which negatively correlates with the germination rate. The transcriptome profiles of P. armeniacum seed at different development stages were compared to explore the molecular clues for non-methylated lignin synthesis. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that a large number of genes associated with phenylpropanoid biosynthesis and phenylalanine metabolism during seed maturation were differentially expressed. Several key genes in the lignin biosynthetic pathway displayed different expression patterns during the lignification process. PAL, 4CL, HCT, and CSE upregulation was associated with C and H lignin accumulation. The expression of CCoAOMT, F5H, and COMT were maintained at a low level or down-regulated to inhibit the conversion to the typical G and S lignin. Quantitative real-time RT-PCR analysis confirmed the altered expression levels of these genes in seeds and vegetative tissues. Conclusions: This work demonstrated the plasticity of natural lignin polymer assembly in seed and provided a better understanding of the molecular mechanism of seed-specific lignification process.
Tumor necrosis as a poor prognostic predictor on postoperative survival of patients with solitary small hepatocellular carcinoma
Authors: Ling Yi-hong , Chen Jie-wei , Wen Shi-hong , Huang Chao-yun , Li Peng , Lu Liang-he , Mei Jie , Li Shao-hua , Wei Wei , Cai Muyan , Guo Rong-ping
Published: June 2020

Background: Small hepatocellular carcinoma (sHCC) is a special subtype of HCC with the maximum tumor diameter ≤ 3 cm and favorable long-term outcomes. Surgical resection or radiofrequency ablation offer the greatest chance for cure; however, many patients still undergo tumor recurrence after primary treatment. So far, there is no clinical applicable method to assess biological aggressiveness in solitary sHCC. Methods: In the present study, we retrospectively evaluated tumor necrosis of 335 patients with solitary sHCC treated with hepatectomy between December 1998 and 2010 from Sun Yat-sen University Cancer Center. Results: In the current study, the presence of tumor necrosis was observed in 157 of 335 (46.9%). Further correlation analysis showed that the presence of tumor necrosis in sHCC was significantly correlated with tumor size and vascular invasion (P = 0.026, 0.003, respectively). The presence of tumor necrosis was associated closely with poorer cancer-specific overall survival (OS) and recurrence-free survival (RFS) as evidenced by univariate (P < 0.001;hazard ratio, 2.821; 95% CI, 1.643-4.842) and multivariate analysis (P = 0.005; hazard ratio, 2.208; 95% CI, 1.272-3.833). More importantly, the combined model by tumor necrosis, vascular invasion and tumor size can significantly stratify the risk for RFS and OS and improve the ability to discriminate sHCC patients’ outcomes (P < 0.0001 for both). Conclusions: Our findings provide evidence that tumor necrosis has the potential to be a parameter for cancer aggressiveness in solitary sHCC. The combined prognostic model may be a useful tool for identifying solitary sHCC patients with worse outcomes.
Aerobic physical activity and muscle-strengthening exercise guideline adherence in Germany: findings from a national sample of 24,016 adults
Authors: Bennie Jason , Cocker Katrien De , Tittlbach Susanne
Published: June 2020

Background The German ‘National Recommendations for Physical Activity and Physical Activity Promotion’ state that adults (≥18 years) should engage in: [i] ≥150 minutes of aerobic moderate-to-vigorous-intensity physical activity/week (MVPA); and [ii] ≥2 days/week of muscle-strengthening exercise (MSE). However, there is limited research on the adherence to these guidelines among German adults. The present purpose was to describe the prevalence and correlates of physical activity guideline adherence among a nationally representative sample of German adults. Methods Data were drawn from the 2014 German Health Update survey, collected via a combination of web-based and mail surveys. Self-reported physical activity levels were assessed using the previously validated European Health Interview Survey Physical Activity Questionnaire. Weighted prevalence levels of the sample meeting the aerobic MVPA (≥150 minutes/week), MSE (≥2 times/week) and combined MVPA-MSE guidelines were calculated. Poisson regressions were used to assess prevalence ratios for physical activity guideline adherence categories across sociodemographic (age, sex, socioeconomic status) and lifestyle-related (self-rated health, BMI) variables. Results Out of 24,016 participants (response rate = 27.6%), aged ≥18 years, 45.3% (95% CI: 44.5-46.0%), 29.4% (95% CI: 28.7-30.1%) and 22.6% (95% CI: 21.9-23.2%) met the aerobic MVPA, MSE and combined guidelines, respectively. Population sub-groups independently less likely to meet the combined guidelines included those with poor self-rated health, low socioeconomic status and those being overweight or obese. Conclusions As almost 80% of German adults do not meet the nationally recommended combined aerobic MVPA-MSE physical activity guidelines, there is a necessity for large-scale public health interventions promoting both aerobic MVPA and MSE.
TANA: efficient approach for predicting protein functions by transferring annotation via alignment networks
Authors: DJEDDI Warith Eddine , YAHIA Sadok BEN , NGUIFO Engelbert MEPHU
Published: June 2020

Background: One of the challenges of the post-genomic era is to provide accurate function annotations for orphan and unannotated protein sequences. With the recent availability of huge PPI networks for many model species, the computational methods revealed a great requirement to elucidate protein function based on many strategies. In this respect, most computational approaches integrate diverse kinds of functional interactions to unveil protein functions by transferring annotations across different species by relying on a similar sequence, structure 2D/3D, amino acid patterns of phylogenetic profiles. Results: In this work, we introduce a new approach, called TANA, for inferring protein functions. The main originality of the introduced approach stands on the function prediction for the unannotated protein by transferring annotation via a network alignment as well as from the direct interaction neighborhood within their PPI networks. In doing so, we are able to discover the functions of proteins that could not be easily described by sequence homology. We assess the performance of our approach using the standard metrics established by the CAFA challenge and highlight a sharp significant improvement over other competitive methods, in particular for predicting molecular functions and cellular components. Conclusions: This research is one of the first attempts that combine sequence and networks-multiple-alignment-based function prediction approaches. We have been able to assess the accuracy of the prediction using pairwise and multiple alignment of the PPI networks for the compared species. Therefore, we recommend using different strategies (i.e. pairwise, multiple, with/without neighborhood networks) especially in situations where the functions of the protein are not known beforehand
Experience of Management of Anorexia Nervosa Patients With Extremely Severe Malnutrition In A Transdisciplinary Eating Disorders Inpatient Unit
Authors: Guinhut Marie , Melchior Jean-Claude , Godart Nathalie , Hanachi Mouna
Published: June 2020

Background: We describe a transdisciplinary medical unit, referral center for the management of anorexia nervosa (AN) adult patients with extremely severe malnutrition. Method: History, organization and activity of the unit are described. Characteristics of all adult AN patients admitted for the first time in the unit between November 1997 and January 2014 and medical care provided are detailed, before discussing the value of such a unit.Results: The unit was established as an independent department in 1997 and has 180 to 200 admissions per year. The patients hospitalized in the department are clinically serious and unstable because of life-threatening somatic complications due to a low BMI (< 13 kg/m²). They are referred on criteria of somatic severity. They often had multiple hospitalizations in other acute care hospitals before being supported in the unit. Among a cohort of 386 adult AN patients admitted for the first time in the unit, mean age was 29.4 (± 11.5) years, mean BMI was 12.7 (± 2.2) kg/m² and 78.2% of patients had already been hospitalized in other hospitals. Intensive medical care, both somatic and psychiatric, are provided thanks to a transdisciplinary therapeutic program, where objectives are to optimize care and enable a therapeutic alliance between patient and caregiver, The goals of treatment are to medically stabilize the patient, to initiate progressive refeeding and to start supportive psychotherapy before being transferred to a psychiatric eating disorder unit.Conclusion: This specialized transdisciplinary unit where physician nutritionists and psychiatrists coordinate medical care together, allow a better understanding and management of extreme malnutrition associated with AN. Thanks to their expertise, care teams are less critical and less rejecting towards patients. Thus, therapeutic alliance could be optimized.
Parental mental health conditions and use of healthcare services in children the first year of life – a register-based, nationwide study
Authors: Heuckendorff Signe , Johansen Martin Nygård , Johnsen Søren Paaske , Overgaard Charlotte , Fonager Kirsten
Published: June 2020

Background Parental mental health conditions have been associated with increased morbidity and use of healthcare services in offspring. Existing studies have not examined different severities of parental mental health conditions, and the impact of paternal mental health has been overlooked.We examined the association between two severities of parental mental health conditions and use of healthcare services for children during the first year of life and explored the impact of both maternal and paternal mental health conditions. Methods This register-based cohort study included all live-born children born in Denmark from 2000-2016. Information on socioeconomics, diagnoses, drug prescriptions, and healthcare contacts was extracted from nationwide public registries. Parents were grouped according to severity of mental condition based on the place of treatment of the mental health condition. Poisson regression analyses were performed to estimate the incidence rate ratio (IRR) of contacts to general practice (GP), out-of-hour medical service, emergency room (ER), and out- and inpatient hospital contacts during the first 12 months of the child’s life.Results The analyses included 964395 children. Twenty percent of the mothers and twelve percent of the fathers were identified with mental health conditions. Paternal mental health conditions were independently associated with increased risk of infant healthcare contacts (GP IRR 1.05 (CI95% 1.04-1.05) and out-of-hour IRR 1.20 (CI95% 1.18-1.21)). Risks were higher for maternal mental health conditions (GP IRR 1.17 (CI95% 1.17-1.18) and out-of-hour IRR 1.38 (CI95% 1.37-1.37)). The risks were even higher if both parents were classified with a mental health condition (GP IRR 1.24 (CI95% 1.23-1.25) and out-of-hour contacts IRR 1.48 (CI95% 1.45-1.51)), including minor mental health condition (GP IRR 1.22 (CI95% 1.22-1.23) and out-of-hour IRR 1.37 (CI95% 1.35-1.39)). This pattern was the same for all types of healthcare contacts.Conclusions Both maternal and paternal mental health conditions, including minor mental health conditions, were associated with increased utilization of healthcare services. Focus on both parents’ mental health conditions (even if minor) may be warranted in service planning.
Modeling Count Data for Healthcare Utilization: an Empirical Study of Outpatient Visits Among Vietnamese Older People
Authors: Le Dung Duc , Leon-Gonzalez Roberto , Matola Joseph Upile
Published: June 2020

Background

Vietnam is undergoing an unprecedented pace of aging process and is expected to experience the fastest aging process in region. Association between increasing age and health deterioration has been well-documented across settings. Consequently, demand for healthcare utilization is rising among older people. However, healthcare utilization, here measured as count data, creates challenges for modeling because such data typically has distributions that are skewed with a large mass at zero. This study compares empirical econometric strategies for the modeling of healthcare utilization (measured as the number of outpatient visits in the last 12 months), and identifies the determinants of healthcare utilization among Vietnamese older people based on the best-fitting model identified.
Methods

Using the Vietnam Household Living Standard Survey in 2006 (N = 2426), nine econometric regression models for count data were examined to identify the best-fitting one. We used model selection criteria; statistical tests; and goodness-of-fit for in-sample model selection. In addition, we conducted 10-fold cross-validation checks to examine reliability of in-sample model selection. Finally, we utilized marginal effects to identify the factors associated with number of outpatient visits among Vietnamese older people based on the best-fitting model identified.
Results

We found strong evidence in favor of hurdle negative binomial model 2 (HNB2) for both in-sample selection and 10-fold cross-validation checks. The marginal effect results of the HNB2 showed that predisposing, enabling, need, and lifestyle factors were significantly associated with number of outpatient visits. The predicted probabilities for each count event showed the distinct trends of healthcare utilization among specific groups: at low count events, women and people in younger age group used more healthcare utilization than did men and their counterparts in older age groups, but a reversed trend was found at higher count events.
Conclusions

The findings here suggest that the HNB2 model should be considered for use in modeling counts of healthcare use. This study’s findings lay the groundwork for future research on the modeling of healthcare utilization in developing countries and those findings could be used to forecast on healthcare demand and making provisions for healthcare costs.
Comparative proteomic analysis of thick-walled ray formation of Haloxylon ammodendron in the Gurbantunggut Desert, China
Authors: Zhou Chaobin , Ding Junjie , Hu Xiaojing , Gong Wei
Published: June 2020

Background

The thick-walled ray cells have been reported in Haloxylon ammodendron for the first time. This study measured the wall thickness of ray cells and performed a proteomic analysis of ray cell wall formation in the xylem of H. ammodendron using isobaric tags for relative and absolute quantitation.
Results

The wall thickness of ray cells in Jinghe (2.85 ± 0.42 µm) was significantly lower than that in Shihezi (3.08 ± 0.44 µm) (P < 0.01). In Shihezi, which has a thicker wall of ray cells than that in Jinghe, 795 differentially expressed proteins were upregulated. Phenylpropanoid biosynthesis, photosynthesis, glycolysis/gluconeogenesis, carbon metabolism, starch and sucrose metabolism, metabolic pathways, etc. promote ray cell wall biosynthesis of the xylem of H. ammodendron by providing substrates or energy. During the process of cell wall biosynthesis in the xylem of H. ammodendron, the nonspecific lipid-transfer protein and beta expansin EXPB2.1 (Mirabilis jalapa] first loosens the cell wall, followed by extension and expansion, and the xyloglucan endotransglycosylase/hydrolase 1 cleaves and links the xyloglucan chains. Then, photosystem I P700 apoprotein A1, reversibly glycosylated polypeptide 1 and GDP-mannose-3′, 5′-epimerase, etc., are involved in cellulose, hemicellulose and pectin biosynthesis of the cell wall by providing components or energy. Finally, the proteins in phenylpropanoid biosynthesis promote the lignification of the ray cell wall and complete the biosynthetic process of the cell wall.
Conclusions

Phenylpropanoid biosynthesis, photosynthesis, glycolysis/gluconeogenesis, carbon metabolism, starch and sucrose metabolism, metabolic pathways, etc. promote ray cell wall biosynthesis of the xylem of H. ammodendron by providing substrates or energy. The results are important for improving the wood mechanical properties of timber plantations.
Bioinformatic Profiling Identifies Prognosis-Related Genes in the Immune Microenvironment of Endometrial Carcinoma
Authors: Cheng Pu , Ma Jiong , Zheng Xia , Zhou Chunxia , Chen Xuejun
Published: June 2020

Background: Endometrial carcinoma (EC) is a common malignancy of female genital system which exhibits a unique immune profile. It is a promising strategy to quantify immune patterns of EC for predicting prognosis and therapeutic efficiency. Here, we attempted to identify the possible immune microenvironment-related prognostic markers of EC. Methods: We obtained the RNA sequencing and corresponding clinical data of EC from TCGA database. Then, 3 immune scores based on the Estimation of STromal and immune cells in MAlignant Tumor tissues using Expression data (ESTIMATE) algorithm were computed. Correlation between above ESTIMATE scores and other immune-related scores, molecular subtypes, prognosis, and gene mutation status (including BRCA and TP53) were further analyzed. Afterwards, gene modules associated with the ESTIMATE scores were screened out through hierarchical clustering analysis and WGCNA. Differentially expressed analysis was performed and genes shared by the most relevant modules were found out. KEGG pathway enrichment analysis was conducted to explore the biological functions of those genes. Survival analysis was carried out to identify prognostic immune-related genes and GSE17025 database was used as external dataset for further validation.Results: The immune-related scores based on ESTIMATE algorithm was closely related to the immune microenvironment of EC. 3 gene modules that had the closest correlations with 3 ESTIMATE scores were obtained. 109 immune-related genes were preliminarily found out and 29 pathways were significantly enriched, most of which were associated with immune response. Univariate survival analysis revealed that there were 14 genes positively associated wicth both OS and PFS. Among which, 11 genes showed marked correlations with ImmuneScore values in GSE17025 database.Conclusion: Our current study profiled the immune status and identified 14 novel immune-related prognostic biomarkers for EC. Our findings may help to investigate the complicated tumor microenvironment and develop novel individualized therapeutic targets for EC.
To Established Hematological Reference Intervals Among Full-Term Newborn Babies at University of Gondar Comprehensive Specialized Hospital, Northwest Ethiopia: A Cross-Sectional Study
Authors: Tiruneh Tegenaw , Kiros Teklehymanot , Getu Sisay
Published: June 2020

Background: Hematological reference intervals are used for medical decision tools for interpretation of numerical test results. Establishing of hematological interval among newborn babies is very important for the diagnosis of malignancy, anemia, bleeding disorders, and various infections. There is no locally established hematological reference intervals in Ethiopia. Thus the aim of this study is to established locally determined hematological reference interval among full-term newborn babies.Methods: A cross sectional study was conducted from February to May 2019 among 151 apparently healthy full-term newborn babies at Gondar University Hospital. About 3ml of cord blood was obtained for analysis of Hematological parameters and determined by using Sysmex KX-21N (Sysmex Corporation Kobe, Japan) automated analyzer. Median, 2.5th and 97.5th percentiles were computed. Result: Males to female’s ratio was almost equal. All hematological parameter had no statistically significant difference between males and females. The delivery types were not influenced its hematological values. Conclusion: Hematological reference interval was established from full-term newborn babies at University of Gondar hospital and was different from other studies in African and Europeans. Therefore, own determined reference value is very important for the clinicians to correctly diagnosis the patients at health facility levels.
Clinical Features of Pregnant Women and Neonates with COVID-19 in Wuhan: a paired comparison study
Authors: Xie Qin , Gan Quan , Hu Jing , Zhou Dong , Cai Shuhan , Su Lianjiu , Li Yunxiang , Li Yiming , Peng Zhiyong
Published: June 2020

Background

Our study aimed to find symptoms unique in pregnancy and to help the early diagnosis in pregnant women and to evaluate the impact of COVID-19 in postpartum women and their newborns.
Methods

Clinical data were reviewed and collected for 11 pregnant women with laboratory-confirmed COVID-19 who were consecutively admitted to Zhongnan Hospital of Wuhan University and Women and Children’s Hospital of Hubei Province, from Jan 26 to Feb 26, 2020.
Results

All the confirmed women didn’t have any exposure history and their early symptoms were mildly elevated temperate and fatigue. The chest CT scans of confirmed women can be atypical manifestations, such as bilateral pleural effusions and slightly increased densities. Eight of eleven confirmed women did not feel anything unusual until abnormalities were found on chest CT scans on admission screening test. All three groups had elevated white blood cell count, neutrophil count and lactate dehydrogenase, and reduced total protein.The infection did not increase the risk for premature delivery, premature rupture of membrane, or comorbidities in pregnancy.
Conclusions

Pregnant women were often asymptomatic and accidentally detected abnormalities on chest CT scan on admission which emphasize the importance of CT scan in prevalent areas of the COVID-19. Even after the laboratory confirmation, the manifestation of the CT scan could be atypical, which alerted the necessity of protection for healthcare workers. The COVID-19 did not increase the risk of complications in pregnant women and their neonates.
Trial registration:

This case series was approved by the institutional ethics board of Zhongnan Hospital of Wuhan University (No. 2020020) and Women and Children’s Hospital of Hubei Province(NO. LW035).
Reduced-dose of Posttransplant Cyclophosphamide and Cotransplantation of Peripheral Blood Stem Cells and Human Umbilical Cord-derived Mesenchymal Stem Cells in Severe Aplastic Anemia
Authors: Zu Yingling , Zhou Jian , Fu Yuewen , Fang Baijun , Liu Xinjian , Zhang Yanli , Yu Fengkuan , Zuo Wenli , Zhou Hu , Gui Ruirui , Li Zhen , Liu Yanyan , Zhao Huifang , Zhang Chengjuan , Song Yongping
Published: June 2020

Background Posttransplant cyclophosphamide (PTCY) as graft-versus-host disease (GVHD) prophylaxis is an effective strategie for patients receiving matched sibling donor hematopoietic stem cell transplantation (MSD-HSCT) and haploidentical HSCT (haplo-HSCT). Methods We evaluated the effectiveness and safety of reduced-dose cyclophosphamide, 20 mg/kg for 13 patients in the MSD-HSCT group and 25 mg/kg for 22 patients in the haplo-HSCT group, on days +3 and +4 combined with cotransplantation of peripheral blood stem cells (PBSCs) and human umbilical cord-derived mesenchymal stem cells (UC-MSCs) for patients with severe aplastic anemia (SAA) retrospectively. Results In the MSD-PTCY group, the times to neutrophil and platelet engraftment were significantly shorter than those in the MSD-control group (P<0.05), 11 (range 10 to 14) days and 12 (range 9 to 15) days. The cumulative incidence of acute GVHD (aGVHD) at day +100 (15.4%) was lower in the MSD-PTCY group than in the MSD-control group(P=0.050). No patient developed chronic GVHD (cGVHD). The 1-year overall survival (OS) and event-free survival (EFS) rates in the MSD-PTCY group were 100% and 92.3%. In the haplo-PTCY group, the times to neutrophil and platelet engraftment were significantly shorter than those in the haplo-control group (P<0.05), 12 (range 9 to 15) days and 11.5 (range 9 to 17) days. The cumulative incidences of aGVHD at day +100 and 1-year cGVHD in the haplo-PTCY group were 31.8% and 18.2%. The 1-year OS and EFS rates in the haplo-PTCY group were 81.8% and 66.9%. Conclusions Reduced-dose PTCY and cotransplantation of PBSCs and UC-MSCs is feasible for patients with SAA, especially for overcoming the high incidences of aGVHD and cGVHD due to PBSCs.
An Investigation of Construct Validity and Responsiveness of the Danish ICECAP-A
Authors: Holst-Kristensen Annette Willemoes , Mitchell Paul Mark , Linton Myles-Jay , Coast Joanna , Fonager Kirsten , Pedersen Kjeld Møller
Published: June 2020

BackgroundThis study aimed to provide the first assessment of construct validity of ICECAP-A in patients with cardiovascular disease, chronic obstructive pulmonary disease and diabetes, and to assess the responsiveness of the measure in this group. MethodData were provided from patients attending rehabilitation in the municipality of Aalborg, Denmark, from March 2018 to March 2019. Patients answered a questionnaire from the healthcare centre and the ICECAP-A at baseline and 12 weeks follow-up. To assess construct validity, a priori hypotheses were developed. Based on these hypotheses, associations between sociodemographic characteristics, ‘general health’, a freedom dimension, and ICECAP-A were analysed through chi-squared tests and Spearman rank correlations for categorical and ordinal variables, respectively. To investigate responsiveness, the anchor-based method was used. Patients were divided into improved, worsened or no change, based on changes between baseline and follow-up on the anchor measures (‘general health’ and ‘freedom’). To quantify responsiveness, both the weighted and un-weighted ICECAP-A scores’ effect sizes, standardised response means and t-tests were used. Findings were explored across different age groups.ResultOf all the hypothesised associations, 16 of 26 (62%) were in the expected direction. The expected relationships were found between ICECAP-A scores and general health and freedom to do things. ICECAP-A was responsive in terms of capturing the effects on general health and the freedom to do things. Differences were found across age groups, with greater responsiveness to change in those aged under 65 years. The item-by-item analysis showed that capability was mainly driven by stability and autonomy.ConclusionThis study has shown that the Danish ICECAP-A is a valid and responsive measure of the effects of an exercise and education-based rehabilitation programme.
Temporal Heterogeneity of ROS1 Fusion and Braf Fusion Conferred Resistance to First-and Third-generation EGFR Tkis: a Case Report.
Authors: Zhang Meng , Huang Yihua , Chen Baorong , Peng Yuna , Lin Jing , Li Yuan , Chen Shuyin , Guan Liya , Yao Guohua , Fang Wenfeng
Published: June 2020

Background: The development of EGFR-tyrosine kinase inhibitors (EGFR-TKIs) have revolutionized the treatment paradigms for non-small cell lung cancer (NSCLC) patients. However, acquired resistance inevitably develops with a median progression-free survival (PFS) less than a year. The advancements in sequencing technologies have significantly promoted the identification of potential resistant mechanisms to targeted therapies.Case presentation: A 39-year-old Chinese male was diagnosed stage IVB NSCLC with EGFR exon 19 deletion (EGFR 19del) and received icotinib but got primary EGFR-TKI resistance caused by ROS1 fusion one month later. Then he received icotinib plus crizotinib and achieved partial response with a profession free survival of 4.8 months. Then he switched to osimertinib plus crizotinib due to the emergence of EGFR T790M mutation. TRIM24-BRAF fusion was detected after osimertinib failure, which might be potential resistant mechanism to osimertinib.Conclusions: This is the first case to confirm crizotinib plus icotinib in patients harboring both EGFR 19del and ROS1 fusion. This case displays the temporal heterogeneity of different resistant mechanisms emerging during disease course, highlighting the importance of dynamic genetic monitoring for better clinical managements.