bluuebunny/crossref_metadata_2025 huggingface dataset:
National Identities in Troubled Times: Germany and Southern European Countries after the Great Recession
Authors: Ruiz Jiménez Antonia María , Aquino Llinares Nieves , Ferri Fuentevilla Elena
Published: April 2021

This article aims to elucidate the effects of the Great Recession and the retrenchment of welfare on national identity in several European countries. While different authors have observed that good economic performance, redistribution, and the growth of welfare strengthen countries as political communities of solidarity, there is much less empirical evidence regarding the consequences of an economic crisis for national identity. To investigate these consequences, we focus on a set of countries where the 2008 Great Recession resulted in different impacts, namely, Germany and four countries in Southern Europe (Italy, Spain, Portugal, and Greece). We use secondary quantitative data from Eurobarometer surveys to test aggregated and individual hypotheses relating to both the size and direction of the Great Recession’s effects on national identity. Our results suggest that the roles and impacts of economic variables may be different depending on the relative economic performance of a country within its own context. It seems easier to confirm that good economic performance, in relative terms, might strengthen national identity than proving that poor economic performance will weaken national identity. Even if no definitive empirical evidence can be given at this point, our data suggest a rationalization or compensation mechanism such that citizens look for where to anchor their strong national identities after they have decided on them. If an economy is performing well, then it would become a good anchorage for holding a strong national identity; however, if an economy is not performing well, then economic factors will cease to be a fundamental element for national identity holders.
The Role of Place of Delivery in Preventing Neonatal and Infant Mortality Rate in India
Authors: Das Ujjwal , Chaplot Barkha , Azamathulla Hazi Mohammad
Published: April 2021

Skilled birth attendance and institutional delivery have been advocated for reducing maternal, neonatal mortality and infant mortality (NMR and IMR). This paper examines the role of place of delivery with respect to neo-natal and infant mortality in India using four rounds of the Indian National Family Health Survey conducted in 2015–2016. The place of birth has been categorized as “at home” or “public and private institution.” The role of place of delivery on neo-natal and infant mortality was examined by using multivariate hazard regression models adjusted for clus-tering and relevant maternal, socio-economic, pregnancy and new-born characteristics. There were 141,028 deliveries recorded in public institutions and 54,338 in private institutions. The esti-mated neonatal mortality rate in public and private institutions during this period was 27 and 26 per 1000 live births respectively. The study shows that when the mother delivers child at home, the chances of neonatal mortality risks are higher than the mortality among children born at the health facility centers. Regression analysis also indicates that a professionally qualified provider′s antenatal treatment and assistance greatly decreases the risks of neonatal mortality. The results of the study illustrate the importance of the provision of institutional facilities and proper pregnancy in the prevention of neonatal and infant deaths. To improve the quality of care during and imme-diately after delivery in health facilities, particularly in public hospitals and in rural areas, accel-erated strengthening is required.
Dietary Exposures and Intake Doses to Bisphenol A and Triclosan in 188 Duplicate-Single Solid Food Items Consumed by US Adults
Authors: Morgan Marsha K. , Clifton Matthew S.
Published: April 2021

Few data exist on bisphenol A (BPA) or triclosan (TCS) residue levels in foods consumed by adults in everyday settings. In a further analysis of study data, the objectives were to determine BPA and TCS residue concentrations in duplicate-single solid food items consumed by adults and to estimate dietary exposure and intake doses per food item. A convenience sample of 50 adults was recruited in North Carolina (2009–2011). Participants completed 24 h food diaries and collected 24 h duplicate-diet solid food samples consumed on days 1 and 2 during sampling weeks 1, 2, and 6. A total of 188 of the collected 776 duplicate-diet solid food samples contained a single, solid food item. BPA and TCS residue levels were quantified in the 188 food items using GC–MS. BPA and TCS were detected in 37% and 58% of these food items, respectively. BPA concentrations were highest in a cheese and tomato sandwich (104 ng/g), whereas the highest TCS concentrations were in a burrito (22.1 ng/g). These chemicals co-occurred in 20% of the samples (maximum = 54.7 ng/g). Maximum dietary intake doses were 429 ng/kg/day for BPA in a vegetable soup with tortilla sample and 72.0 ng/kg/day for TCS in a burrito sample.
Evaluation of a Human–Machine Interface for Motion Sickness Mitigation Utilizing Anticipatory Ambient Light Cues in a Realistic Automated Driving Setting
Authors: Hainich Rebecca , Drewitz Uwe , Ihme Klas , Lauermann Jan , Niedling Mathias , Oehl Michael
Published: April 2021

Motion sickness (MS) is a syndrome associated with symptoms like nausea, dizziness, and other forms of physical discomfort. Automated vehicles (AVs) are potent at inducing MS because users are not adapted to this novel form of transportation, are provided with less information about the own vehicle’s trajectory, and are likely to engage in non-driving related tasks. Because individuals with an especially high MS susceptibility could be limited in their use of AVs, the demand for MS mitigation strategies is high. Passenger anticipation has been shown to have a modulating effect on symptoms, thus mitigating MS. To find an effective mitigation strategy, the prototype of a human–machine interface (HMI) that presents anticipatory ambient light cues for the AV’s next turn to the passenger was evaluated. In a realistic driving study with participants (N = 16) in an AV on a test track, an MS mitigation effect was evaluated based on the MS increase during the trial. An MS mitigation effect was found within a highly susceptible subsample through the presentation of anticipatory ambient light cues. The HMI prototype was proven to be effective regarding highly susceptible users. Future iterations could alleviate MS in field settings and improve the acceptance of AVs.
Antithrombotic Management and Long-Term Outcomes of Patients with Atrial Fibrillation. Insights from CRAFT Trial
Authors: Balsam Paweł , Lodziński Piotr , Gawałko Monika , Kraj Leszek , Śliwczyński Andrzej , Maciejewski Cezary , Krzowski Bartosz , Tymińska Agata , Ozierański Krzysztof , Grabowski Marcin , Bednarski Janusz , Opolski Grzegorz
Published: April 2021

Background: We aimed to compare long-term outcomes in Polish patients with atrial fibrillation (AF) according to oral anticoagulation (OAC) type and to evaluate the predictive value of common thromboembolic and bleeding risk scores. Methods: Data from the CRAFT trial (NCT02987062) were included. The primary study endpoint was major adverse event (MAE; all-cause death, thromboembolic and hemorrhagic event) during the mean four-year follow-up period. Results: Out of 2983 patients with available follow-up data, 1686 (56%) were prescribed with vitamin K antagonist (VKA), 891 (30%) with rivaroxaban and 406 (14%) with dabigatran. Predominance of elderly and female patients with previous history of thromboembolic and hemorrhagic events was observed within rivaroxaban (vs. other OAC) group. Higher rate of MAEs and its components was observed in patients on VKA followed by rivaroxaban as compared to patients on dabigatran (43% vs. 42% vs. 31%, p < 0.01). After group matching based on clinical characteristics, higher risk of hemorrhagic events in VKA (vs. dabigatran) and rivaroxaban (vs. dabigatran) group were observed. The available thromboembolic (CHA2DS2-VASs, ATRIA, R2CHADS2) and bleeding (HAS-BLED, ATRIA, ORBIT) risk scores showed poor prediction value. Conclusions: Despite no difference in the thromboembolic event rate, treatment with VKA and rivaroxaban was associated with a significant increase in the risk of hemorrhagic events.
Role of Proteasomes in Inflammation
Authors: Goetzke Carl Christoph , Ebstein Frédéric , Kallinich Tilmann
Published: April 2021

The ubiquitin–proteasome system (UPS) is involved in multiple cellular functions including the regulation of protein homeostasis, major histocompatibility (MHC) class I antigen processing, cell cycle proliferation and signaling. In humans, proteasome loss-of-function mutations result in autoinflammation dominated by a prominent type I interferon (IFN) gene signature. These genomic alterations typically cause the development of proteasome-associated autoinflammatory syndromes (PRAAS) by impairing proteasome activity and perturbing protein homeostasis. However, an abnormal increased proteasomal activity can also be found in other human inflammatory diseases. In this review, we cast a light on the different clinical aspects of proteasomal activity in human disease and summarize the currently studied therapeutic approaches.
Bidirectional Association between Metabolic Control in Type-2 Diabetes Mellitus and Periodontitis Inflammatory Burden: A Cross-Sectional Study in an Italian Population
Authors: Romano Federica , Perotto Stefano , Mohamed Sara Elamin Osman , Bernardi Sara , Giraudi Marta , Caropreso Paola , Mengozzi Giulio , Baima Giacomo , Citterio Filippo , Berta Giovanni Nicolao , Durazzo Marilena , Gruden Gabriella , Aimetti Mario
Published: April 2021

This study assessed the periodontal conditions of type 2 diabetes (T2DM) patients attending an Outpatient Center in North Italy and explored the associations between metabolic control and periodontitis. Periodontal health of 104 T2DM patients (61 men and 43 women, mean age of 65.3 ± 10.1 years) was assessed according to CDC/AAP periodontitis case definitions and Periodontal Inflamed Surface Area (PISA) Index. Data on sociodemographic factors, lifestyle behaviors, laboratory tests, and glycated hemoglobin (HbA1c) levels were collected by interview and medical records. Poor glycemic control (HbA1c ≥ 7%), family history of T2DM, and C-reactive protein levels were predictors of severe periodontitis. An increase in HbA1c of 1% was associated with a rise in PISA of 89.6 mm2. On the other hand, predictors of poor glycemic control were severe periodontitis, waist circumference, unbalanced diet, and sedentary lifestyle. A rise in PISA of 10 mm2 increased the odds of having HbA1c ≥ 7% by 2%. There is a strong bidirectional connection between periodontitis and poor glycemic control. The inflammatory burden posed by periodontitis represents the strongest predictor of poor glycemic control.
Clinical Characteristics of Primary Repair for Perforated Peptic Ulcer: 10-Year Experience in a Single Center
Authors: Choi Yun-Suk , Heo Yoon-Seok , Yi Jin-Wook
Published: April 2021

Background: Perforated peptic ulcer (PPU) is a disease whose incidence is decreasing. However, PPU still requires emergency surgery. The aim of this study was to review the clinical characteristics of patients who received primary repair for PPU and identify the predisposing factors associated with severe complications. Method: From January 2011 to December 2020, a total of 75 patients underwent primary repair for PPU in our hospital. We reviewed the patients’ data, including general characteristics and perioperative complications. Surgical complications were evaluated using the Clavien-Dindo Classification (CDC) system, with which we classified patients into the mild complication (CDC 0–III, n = 61) and severe complication (CDC IV–V, n = 14) groups. Result: Fifty patients had gastric perforation, and twenty-five patients had duodenal perforation. Among surgical complications, leakage or fistula were the most common (5/75, 6.7%), followed by wound problems (4/75, 5.3%). Of the medical complications, infection (9/75, 12%) and pulmonary disorder (7/75, 9.3%) were common. Eight patients died within thirty days after surgery (8/75, 10.7%). Liver cirrhosis was the most significant predisposing factor for severe complications (HR = 44.392, p = 0.003). Conclusion: PPU is still a surgically important disease that has significant mortality, above 10%. Liver cirrhosis is the most important underlying disease associated with severe complications.
Effect of Physiotherapy Treatment in the Autonomic Activation and Pain Perception in Male Patients with Non-Specific Subacute Low Back Pain
Authors: Abuín-Porras Vanesa , Clemente-Suárez Vicente Javier , Jaén-Crespo Gonzalo , Navarro-Flores Emmanuel , Pareja-Galeano Helios , Romero-Morales Carlos
Published: April 2021

Introduction: Physiotherapy treatment is a common intervention for low back pain (LBP) patients. These interventions have been related to physiological effects in the central nervous system. Thus, the aim of this study was to analyze the effect of physiotherapy treatment in patients with LBP in the autonomic nervous system activation and subjective pain perception of patients. Methods: A total of 30 male subjects diagnosed with non-specific subacute LBP received a 50 min session consisting of (a) a manual therapy based on joint mobilization and soft tissues techniques in the lumbo-pelvic area, (b) a stretching program, and (c) motor control exercises of the core muscles. The autonomic modification of participants was assessed prior to and after the physiotherapy treatment. Results: Heart rate variability (HRV) analysis reported a significant increase in average RR (p = 0.001), RMSSD (p = 0.008), LRMSSD (p = 0.001), SDNN (p = 0.005), and PNN50 (p = 0.024) after the session. Frequency-domain measures showed a significant increase in LF (p = 0.030) and HF (p = 0.014), and a decrease in LF/HF ratio (p = 0.046). A significant decrease was found in minimum HR values (p = 0.001) and average HR (p = 0.001). Moreover, maximal HR decreased its value from 116.7 ± 26.1 to 113.7 ± 40.8 after intervention. In addition, subjective pain perception (VAS scores) was significantly lower (p = 0.001) in the post-session assessment. Conclusions: Physiotherapy treatment produced an increase in parasympathetic nervous system activation and a decrease in subjective pain perception in non-specific subacute LBP patients.
Radiological and Clinical Efficacy of Intra-Arterial 90Y-DOTATATE in Patients with Unresectable, Progressive, Liver Dominant Neuroendocrine Neoplasms
Authors: Kolasińska-Ćwikła Agnieszka , Nowicki Mirosław , Sankowski Artur , Pałucki Jakub , Buscombe John , Glinka Lidia , Ćwikła Jarosław
Published: April 2021

This study was performed to determine if intra-arterial (i.a.) administration of 90Y DOTATATE can provide an effective and safe alternative to the accepted standard for i.v. of peptide receptor radionuclide therapy (PRRT) in liver-dominant metastases of gastrointestinal pancreatic neuroendocrine neoplasm (GEP-NEN). A single site, prospective, preliminary case series study included 39 patients with histologically proven liver-dominant NEN. PRRT in the form of 1.15GBq 90Y DOTATATE was given selectively into the liver via radiological catheterization of the hepatic artery, up to four times. The endpoint was radiological response (RECIST). Secondary endpoints assessed clinical well-being post-treatment, progression-free survival (PFS), overall survival (OS), and toxicity. Partial response (PR) was noted in 13% of subjects six weeks post-therapy, increasing to 24% at six months and dropping to 13% at 36 months. Disease progression (DP) was not seen at six weeks, was 5% at six months, and 47% at 36 months. Clinical response based on PS seen in 74% of patients at six weeks, 69% at six months, and 39% at 36 months had PFS and OS, respectively, of 22.7 months and 38.2 months. There was no difference in OS/PFS between those with RECIST PR and SD. One patient had significant toxicity (3%). Use of i.a. PRRT appears to be safe and effective in treating patients with liver-dominant NEN. In addition, the best OS (51 vs. 22 months) was seen when i.a. was used as an upfront treatment of bulky GEP-NEN liver metastases and not after i.v. 90Y DOTATATE. The use of i.a. 90Y DOTATATE PRRT appears to be safe and effective in treating patients with liver-dominant NEN.
Advances in Fabricating the Electrospun Biopolymer-Based Biomaterials
Authors: Wilk Sebastian , Benko Aleksandra
Published: April 2021

Biopolymers formed into a fibrous morphology through electrospinning are of increasing interest in the field of biomedicine due to their intrinsic biocompatibility and biodegradability and their ability to be biomimetic to various fibrous structures present in animal tissues. However, their mechanical properties are often unsatisfactory and their processing may be troublesome. Thus, extensive research interest is focused on improving these qualities. This review article presents the selection of the recent advances in techniques aimed to improve the electrospinnability of various biopolymers (polysaccharides, polynucleotides, peptides, and phospholipids). The electrospinning of single materials, and the variety of co-polymers, with and without additives, is covered. Additionally, various crosslinking strategies are presented. Examples of cytocompatibility, biocompatibility, and antimicrobial properties are analyzed. Special attention is given to whey protein isolate as an example of a novel, promising, green material with good potential in the field of biomedicine. This review ends with a brief summary and outlook for the biomedical applicability of electrospinnable biopolymers.
State-of-the-Art Methods to Improve Energy Efficiency of Ships
Authors: Hüffmeier Johannes , Johanson Mathias
Published: April 2021

Generating energy efficiency through behavioural change requires not only understanding and empathy with user interests and needs but also the fostering of energy saving awareness, a technique and framework that supports operators and ship owners. There is strong potential to make use of different technical solutions to increase energy efficiency, but many cost-efficient solutions relate to carrot-and-stick incentives for operators to minimise energy consumption. These incentives range from voyage planning with weather routing eco-driving bonus, to torque limitations and changes in company policies, all of which demonstrate that the operators’ on-board importance for the energy consumption has been identified. Data collection will allow operators to make better decisions in the lifecycle of the ship from knowledge-driven design to operation, redesign and lifetime extension. Various systems are available for data acquisition, storage and analysis, some of which are delivered by well-known marine suppliers while others are stand-alone systems. The lack of standardization for data capture, transmission and analysis is a challenge, so systematic improvement is required in shipping companies to achieve energy savings. When these are achieved, they will be the result of customer requirements, cost pressure or individual driving forces in the companies. The potential energy savings, brought up in interviews, shows up to 35% on specific routes and up to 60% in specific maneuvers. These savings will be made feasible by operators and crews being involved in the decision-making process.
Two-Channel System Dynamics of the Outer Weser Estuary—A Modeling Study
Authors: Gundlach Jannek , Zorndt Anna , van Prooijen Bram C. , Wang Zheng Bing
Published: April 2021

In this paper, we unravel the mechanisms responsible for the development of the two-channel system in the Outer Weser Estuary. A process-based morphodynamic model is built based on a flat-bed approach using simplified boundary conditions and accelerated morphological development. The results are analyzed in two steps: first, by checking for morphodynamic equilibrium in the simulations and second, by applying a newly developed method that interprets simulations based on categorization of the two-channel system and cross-sectional correlation analysis. All simulations reach a morphodynamic equilibrium and develop two channels that vary considerably over time and between the simulations. Variations can be found in the location and depth of the two channels, the development of the dominant channel over time and the alteration in the dominance pattern. The conclusions are that the development of the two-channel system is mainly caused by the tides and the basin geometry. Furthermore, it is shown that the alternation pattern and period are dependent on the dominance of the tides compared to the influence of river discharge.
A Novel Hybrid Fuel Consumption Prediction Model for Ocean-Going Container Ships Based on Sensor Data
Authors: Hu Zhihui , Zhou Tianrui , Osman Mohd Tarmizi , Li Xiaohe , Jin Yongxin , Zhen Rong
Published: April 2021

Accurate, reliable, and real-time prediction of ship fuel consumption is the basis and premise of the development of fuel optimization; however, ship fuel consumption data mainly come from noon reports, and many current modeling methods have been based on a single model; therefore they have low accuracy and robustness. In this study, we propose a novel hybrid fuel consumption prediction model based on sensor data collected from an ocean-going container ship. First, a data processing method is proposed to clean the collected data. Secondly, the Bayesian optimization method of hyperparameters is used to reasonably set the hyperparameter values of the model. Finally, a hybrid fuel consumption prediction model is established by integrating extremely randomized tree (ET), random forest (RF), Xgboost (XGB) and multiple linear regression (MLR) methods. The experimental results show that data cleaning, the size of the dataset, marine environmental factors, and hyperparameter optimization can all affect the accuracy of the model, and the proposed hybrid model provides better predictive performance (higher accuracy) and greater robustness (smaller standard deviation) as compared with a single model. The proposed hybrid model should play a significant role in ship fuel consumption real-time monitoring, fault diagnosis, energy saving and emission reduction, etc.
Good Practices in Updating Land Information Systems that Used Unconventional Approaches in Systematic Land Registration
Authors: Biraro Mireille , Zevenbergen Jaap , Alemie Berhanu Kefale
Published: April 2021

To properly govern people-to-land relationships, there is a need to formally recognize land rights, and for this to bring recognizable societal change, the established Land Information System (LIS) has to be updated continuously. Though existing literature suggests different parameters to consider when updating an LIS, little is said on how countries are doing this, especially when unconventional approaches through systematic land registration were initially used. This paper comes up with recommendable good practices where the suggested needs for updating land records were made workable. Nine countries with similar data collection procedures for the initial registration were selected based on literature study; questionnaires designed and distributed to LIS experts from each country using internet; and the collected data were analyzed qualitatively. Fortunately, all the case countries possess infrastructure supporting land records updating (procedures, mobilization means, institutional and legal frameworks, and so on). For the majority, the systems are simplified; registration fees are reasonable; services are decentralized; the database is accessible by citizens and local officers; staff are trained; the system effectiveness is assessed; and the political support is offered. However, the procedures are long, data sharing is inexistent, financial and technical sustainability is uncertain, and many different institutions are involved in the registration. Whilst updating used to appear as a forgotten activity, good practices now exist.
New Radical Cation Salts Based on BDH-TTP Donor: Two Stable Molecular Metals with a Magnetic [ReF6]2− Anion and a Semiconductor with a [ReO4]− Anion
Authors: Kushch Nataliya D. , Shilov Gennady V. , Buravov Lev I. , Yagubskii Eduard B. , Zverev Vladimir N. , Canadell Enric , Yamada Jun-ichi
Published: April 2021

Three radical cation salts of BDH-TTP with the paramagnetic [ReF6]2− and diamagnetic [ReO4]− anions have been synthesized: κ-(BDH-TTP)4ReF6 (1), κ-(BDH-TTP)4ReF6·4.8H2O (2) and pseudo-κ″-(BDH-TTP)3(ReO4)2 (3). The crystal and band structures, as well as the conducting properties of the salts, have been studied. The structures of the three salts are layered and characterized by alternating κ-(1, 2) and κ″-(3) type organic radical cation layers with inorganic anion sheets. Similar to other κ-salts, the conducting layers in the crystals of 1 and 2 are formed by BDH-TTP dimers. The partial population of positions of Re atoms and disorder in the anionic layers of 1–3 are their distinctive features. Compounds 1 and 2 show the metallic character of conductivity down to low temperatures, while 3 is a semiconductor. The ac susceptibility of crystals 1 was investigated in order to test the possible slow relaxation of magnetization associated with the [ReF6]2− anion.
Non-Stationary Model of Cerebral Oxygen Transport with Unknown Sources
Authors: Kovtanyuk Andrey , Chebotarev Alexander , Turova Varvara , Sidorenko Irina , Lampe Renée
Published: April 2021

An inverse problem for a system of equations modeling oxygen transport in the brain is studied. The problem consists of finding the right-hand side of the equation for the blood oxygen transport, which is a linear combination of given functionals describing the average oxygen concentration in the neighborhoods of the ends of arterioles and venules. The overdetermination condition is determined by the values of these functionals evaluated on the solution. The unique solvability of the problem is proven without any smallness assumptions on the model parameters.
Characteristics of a Plasticized PVA-Based Polymer Electrolyte Membrane and H+ Conductor for an Electrical Double-Layer Capacitor: Structural, Morphological, and Ion Transport Properties
Authors: Brza Mohamad , Aziz Shujahadeen , Anuar Hazleen , Alshehri Saad , Ali Fathilah , Ahamad Tansir , Hadi Jihad
Published: April 2021

Poly (vinyl alcohol) (PVA)-based solid polymer electrolytes doped with ammonium thiocyanate (NH4SCN) and glycerol were fabricated using a solution casting method. Lithium-based energy storage devices are not environmentally friendly materials, and they are toxic. Thus, proton-conducting materials were used in this work as they are harmless and are smaller than lithium. The interaction between PVA and the electrolyte elements was shown by FTIR analysis. The highest conductivity of 1.82 × 10−5 S cm−1 was obtained by the highest-conducting plasticized system (PSP_2) at room temperature. The mobility, diffusion coefficient, and number density of anions and cations were found to increase with increasing glycerol. FESEM was used to investigate the influence of glycerol on film morphology. TNM showed that the cations and anions were the main charge carriers. LSV showed that the electrochemical stability window of the PSP_2 system was 1.99 V. The PSP_2 system was applied in the preparation of an electrical double layer capacitor device. The shape of the cyclic voltammetry (CV) curve was nearly rectangular with no Faradaic peaks. From the galvanostatic charge-discharge analysis, the power density, energy density, and specific capacitance values were nearly constant beyond the first cycle at 318.73 W/Kg, 2.06 Wh/Kg, and 18.30 F g−1, respectively, for 450 cycles.
Actin and Microtubules Differently Contribute to Vacuolar Targeting Specificity during the Export from the ER
Authors: De Caroli Monica , Barozzi Fabrizio , Renna Luciana , Piro Gabriella , Di Sansebastiano Gian-Pietro
Published: April 2021

Plants rely on both actin and microtubule cytoskeletons to fine-tune sorting and spatial targeting of membranes during cell growth and stress adaptation. Considerable advances have been made in recent years in the comprehension of the relationship between the trans-Golgi network/early endosome (TGN/EE) and cytoskeletons, but studies have mainly focused on the transport to and from the plasma membrane. We address here the relationship of the cytoskeleton with different endoplasmic reticulum (ER) export mechanisms toward vacuoles. These emergent features of the plant endomembrane traffic are explored with an in vivo approach, providing clues on the traffic regulation at different levels beyond known proteins’ functions and interactions. We show how traffic of vacuolar markers, characterized by different vacuolar sorting determinants, diverges at the export from the ER, clearly involving different components of the cytoskeleton.
A Unified Adjustment Model for Gaussian Pulse Welding on Aluminum Alloys
Authors: Zhu Qiang , Yao Ping , Yu Xiaoyan , Xie Bin , Xue Jiaxiang , Hu Haibiao
Published: April 2021

To solve the challenge of welding aluminum alloys, a unified adjustment model for Gaussian pulse welding is established. This model can achieve improved welding performance by adjusting the base current of the weak pulse group within a specific range of average welding current inputs. The flat overlaying welding is carried out on the base material: 6061 aluminum alloys with thicknesses of 2 mm, 3 mm, and 5 mm. A stable welding process, indicated by reduced spatter, is produced, with a soft arc sound and good repeatability in the waveforms of the real-time current and voltage. The weld has a shiny surface and regular fish scale ripples. Metallographic analysis shows that the fusion line is clear, and there are no visible defects, while the weld zone has fine dendritic structures. The tensile test results indicate that fractures occur in the heat-affected zone, and that the tensile strength reaches about 68% of that of the base metal.
Immune Transcriptome of Cells Infected with Enterovirus Strains Obtained from Cases of Autoimmune Thyroid Disease
Authors: Poma Anello Marcello , Hammerstad Sarah Salehi , Genoni Angelo , Basolo Alessio , Dahl-Jorgensen Knut , Toniolo Antonio
Published: April 2021

Background: Hashimoto’s thyroiditis and Graves’ disease are autoimmune thyroid disorders (AITD) of unknown origin. Enterovirus (EV) infection of thyroid cells has been implicated as a possible initiator of cell damage and of organ-specific autoimmunity. We asked whether persistent infection of human epithelial cells with EV strains obtained from thyroid tissue of AITD patients could be associated with transcriptional changes capable of fostering immunopathology. Methods: EV isolates obtained from thyroid tissue of AITD cases were used to infect the AV3 epithelial cell line. AV3 cells incubated with a virus-free medium from thyroid tissue of subjects without evidence of thyroid autoimmunity were used as uninfected controls. Transcripts of immune-related genes were compared in infected vs. uninfected cells. Results: The EV genome and antigens were detected only in the cells exposed to AITD-derived virus isolates, not in control cells. Persistent EV infection, while suppressing transcription of several type I IFN and cytokine determinants, was associated with enhanced transcription of NFKB1/RELA, IFNAR1, JAK1/STAT1, i.e., the determinants that play key immunologic roles. Infection also led to upregulation of the CCL2 chemokine and the IL-18 pro-inflammatory interleukin. Conclusion: As in the case of EV strains obtained from autoimmune diabetes, results show that the EV strains that are present in the thyroid of AITD cases do repress IFN and cytokine pathways. JAK1/STAT1 upregulation supports activation of TLR pathways and aberrant T cell signaling. In the early phases of AITD, our results highlight the potential benefit of interventions aimed at blocking the viral infection and easing the inflammatory response.
Activation of the JNK/MAPK Signaling Pathway by TGF-β1 Enhances Neonatal Fc Receptor Expression and IgG Transcytosis
Authors: Qian Shaoju , Li Chenxi , Liu Xi , Jia Xiangchao , Xiao Yuncai , Li Zili
Published: April 2021

The neonatal Fc receptor (FcRn) transports maternal immunoglobulin G (IgG) to the foetus or newborn and protects the IgG from degradation. FcRn is expressed in several porcine tissues and cell types and its expression levels are regulated by immune and inflammatory events. IPEC-J2 cells are porcine intestinal columnar epithelial cells that were isolated from neonatal piglet mid-jejunum. We hypothesized that transforming growth factor β1 (TGF-β1) upregulated pFcRn expression in IPEC-J2 cells. To test this hypothesis, we treated IPEC-J2 cells with TGF-β1 and demonstrated that porcine FcRn (pFcRn) expression was significantly increased. SP600125, a specific mitogen-activated protein kinase (MAPK) inhibitor, reduced TGF-β1-induced pFcRn expression in IPEC-J2 cells. We performed luciferase reporter assays and showed that the c-JUN sensitive region of the pFcRn promoter gene was located between positions −1215 and −140. The c-JUN sequence, in combination with the pFcRn promoter, regulated luciferase reporter activity in response to TGF-β1 stimulation. Chromatin immunoprecipitation confirmed that there were three c-JUN binding sites in the pFcRn promoter. Furthermore, in addition to increased pFcRn expression, TGF-β1 also enhanced IgG transcytosis in IPEC-J2 cells. In summary, our data showed that the modulation of JNK/MAPK signaling by TGF-β1 was sufficient to upregulate pFcRn expression.
Ambroxol Treatment Suppresses the Proliferation of Chlamydia pneumoniae in Murine Lungs
Authors: Kókai Dávid , Paróczai Dóra , Virok Dezső Peter , Endrész Valéria , Gáspár Renáta , Csont Tamás , Bozó Renáta , Burián Katalin
Published: April 2021

Ambroxol (Ax) is used as a mucolytics in the treatment of respiratory tract infections. Ax, at a general dose for humans, does not alter Chlamydia pneumoniae growth in mice. Therefore, we aimed to investigate the potential anti-chlamydial effect of Ax at a concentration four timed higher than that used in human medicine. Mice were infected with C. pneumoniae and 5-mg/kg Ax was administered orally. The number of recoverable C. pneumoniae inclusion-forming units (IFUs) in Ax-treated mice was significantly lower than that in untreated mice. mRNA expression levels of several cytokines, including interleukin 12 (IL-12), IL-23, IL-17F, interferon gamma (IFN-γ), and surfactant protein (SP)-A, increased in infected mice treated with Ax. The IFN-γ protein expression levels were also significantly higher in infected and Ax-treated mice. Furthermore, the in vitro results suggested that the ERK 1/2 activity was decreased, which is essential for the C. pneumoniae replication. SP-A and SP-D treatments significantly decreased the number of viable C. pneumoniae IFUs and significantly increased the attachment of C. pneumoniae to macrophage cells. Based on our results, a dose of 5 mg/kg of Ax exhibited an anti-chlamydial effect in mice, probably an immunomodulating effect, and may be used as supporting drug in respiratory infections caused by C. pneumoniae.
Revealing the Exciton Fine Structure in Lead Halide Perovskite Nanocrystals
Authors: Hou Lei , Tamarat Philippe , Lounis Brahim
Published: April 2021

Lead-halide perovskite nanocrystals (NCs) are attractive nano-building blocks for photovoltaics and optoelectronic devices as well as quantum light sources. Such developments require a better knowledge of the fundamental electronic and optical properties of the band-edge exciton, whose fine structure has long been debated. In this review, we give an overview of recent magneto-optical spectroscopic studies revealing the entire excitonic fine structure and relaxation mechanisms in these materials, using a single-NC approach to get rid of their inhomogeneities in morphology and crystal structure. We highlight the prominent role of the electron-hole exchange interaction in the order and splitting of the bright triplet and dark singlet exciton sublevels and discuss the effects of size, shape anisotropy and dielectric screening on the fine structure. The spectral and temporal manifestations of thermal mixing between bright and dark excitons allows extracting the specific nature and strength of the exciton–phonon coupling, which provides an explanation for their remarkably bright photoluminescence at low temperature although the ground exciton state is optically inactive. We also decipher the spectroscopic characteristics of other charge complexes whose recombination contributes to photoluminescence. With the rich knowledge gained from these experiments, we provide some perspectives on perovskite NCs as quantum light sources.
Stakeholder Views on the Idea of Medicines Reuse in the UK
Authors: Donyai Parastou , McCrindle Rachel , Hui Terence K. L. , Sherratt R. Simon
Published: April 2021

People’s views about medicines reuse are being examined in a handful of qualitative studies and this commentary adds to that work by drawing on our own discussions with groups of stakeholders in the UK in the past two years. The reuse of medicines within the community pharmacy setting is not permitted in the UK but our multidisciplinary team anticipates that this position will change in the coming years as medication shortages and worries about environmental waste and financial losses from the destruction of unused medicines are brought to the fore. Indeed, for many stakeholders, the issue of waste is a strong feature of conversations about medicines reuse. In addition to this, stakeholders identify the numerous barriers to medicines reuse in the UK. This includes the current uncertainty about the quality of unused medicines returned to pharmacies, which could otherwise be reused. However, stakeholders have also been very willing to propose solutions to a range of existing barriers. Our commentary draws on stakeholder meetings to elaborate the range of views about medicines reuse within a UK context. The challenge is to move forward from these views to advance the technologies that will facilitate medicines reuse practically as well as legally.
Characterizing Proton-Proton Collisions at the Large Hadron Collider with Thermal Properties
Authors: Sahu Dushmanta , Sahoo Raghunath
Published: April 2021

High-multiplicity proton-proton (pp) collisions at the Large Hadron Collider (LHC) energies have created a new domain of research to look for a possible formation of quark–gluon plasma in these events. In this paper, we estimate various thermal properties of the matter formed in pp collisions at the LHC energies, such as mean free path, isobaric expansivity, thermal pressure, and heat capacity using a thermodynamically consistent Tsallis distribution function. Particle species-dependent mean free path and isobaric expansivity are studied as functions of final state charged particle multiplicity for pp collisions at the center-of-mass energy s = 7 TeV. The effects of degree of non-extensivity, baryochemical potential, and temperature on these thermal properties are studied. The findings are compared with the theoretical expectations.
Effect of Different Ratios of Blue and Red LED Light on Brassicaceae Microgreens under a Controlled Environment
Authors: Brazaitytė Aušra , Miliauskienė Jurga , Vaštakaitė-Kairienė Viktorija , Sutulienė Rūta , Laužikė Kristina , Duchovskis Pavelas , Małek Stanisław
Published: April 2021

The consumption of microgreens has increased due to their having higher levels of bioactive compounds and mineral nutrients than mature plants. The lighting conditions during the cultivation of microgreens, if optimally selected, can have a positive effect by further increasing their nutritional value. Thus, our study aimed to determine the changes in mineral nutrients contents of Brassicaceae microgreens depending on different blue–red (B:R) light ratios in light-emitting diode (LED) lighting and to evaluate their growth and nutritional value according to different indexes. Experiments were performed in controlled environment growth chambers at IH LRCAF, 2020. Microgreens of mustard (Brassica juncea ‘Red Lace’) and kale (Brassica napus ‘Red Russian’) were grown hydroponically under different B:R light ratios: 0%B:100%R, 10%B:90%R, 25%B:75%R, 50%B:50%R, 75%B:25%R, and 100%B:0%R. A 220 μmol m−2 s−1 total photon flux density (TPFD), 18 h photoperiod, 21/17 ± 2 °C temperature and 60% ± 5% relative humidity in the growth chamber were maintained during cultivation. We observed that an increasing percentage of blue light in the LED illumination spectrum during growth was associated with reduced elongation in the microgreens of both species and had a positive effect on the accumulation of mostly macro- and micronutrients. However, different B:R light ratios indicate a species-dependent response to changes in growth parameters such as leaf area, fresh and dry mass, and optical leaf indexes such as for chlorophyll, flavonol, anthocyanin, and carotenoid reflectance.
Breeding of High Cooking and Eating Quality in Rice by Marker-Assisted Backcrossing (MABc) Using KASP Markers
Authors: Kim Me-Sun , Yang Ju-Young , Yu Ju-Kyung , Lee Yi , Park Yong-Jin , Kang Kwon-Kyoo , Cho Yong-Gu
Published: April 2021

The primary goals of rice breeding programs are grain quality and yield potential improvement. With the high demand for rice varieties of premium cooking and eating quality, we developed low-amylose content breeding lines crossed with Samgwang and Milkyqueen through the marker-assisted backcross (MABc) breeding program. Trait markers of the SSIIIa gene referring to low-amylose content were identified through an SNP mapping activity, and the markers were applied to select favorable lines for a foreground selection. To rapidly recover the genetic background of Samgwang (recurrent parent genome, RPG), 386 genome-wide markers were used to select BC1F1 and BC2F1 individuals. Seven BC2F1 lines with targeted traits were selected, and the genetic background recovery range varied within 97.4–99.1% of RPG. The amylose content of the selected BC2F2 grains ranged from 12.4–16.8%. We demonstrated the MABc using a trait and genome-wide markers, allowing us to efficiently select lines of a target trait and reduce the breeding cycle effectively. In addition, the BC2F2 lines confirmed by molecular markers in this study can be utilized as parental lines for subsequent breeding programs of high-quality rice for cooking and eating.
Investigating the Invasion Pattern of the Alien Plant Solanum elaeagnifolium Cav. (Silverleaf Nightshade): Environmental and Human-Induced Drivers
Authors: Krigas Nikos , Tsiafouli Maria A. , Katsoulis Georgios , Votsi Nefta-Eleftheria , van Kleunen Mark
Published: April 2021

Invasive alien plant species have impacts on nature conservation, ecosystem services and agricultural production. To identify environmental and human-related drivers of the invasion of Solanum elaeagnifolium (Solanaceae)—one of the worst alien invasive plants worldwide—we conducted an extensive drive-by survey across the Greek territory (presence/absence data; all national major multilane highways; 12–25% of the remaining road network; driven 3–5 times during 2000–2020). These data were linked in GIS with (i) physical environmental attributes (elevation, climate, soil properties) and (ii) type and intensity of human-related activities (land uses, settlements and road type). Compared to previous records, our survey showed that the range of S. elaeagnifolium increased by 1750% during the last decades, doubling its main distribution centers and reaching higher elevations. Our study revealed that the presence of S. elaeagnifolium is associated with (i) higher maximum temperatures and precipitation in summer and low precipitation in winter, as well as with (ii) soil disturbance related to agricultural activities, settlements and road networks, thus facilitating its spread mainly at low altitudes. Our study elucidates the current invasion pattern of S. elaeagnifolium and highlights the urgent need for its widespread monitoring, at least in the noninvaded areas in Greece that have been surveyed in this study. Preventative measures and integrative initiatives should be implemented quickly, and urgently incorporated into current agricultural, road network and conservation-management regimes.
Effects of the Combinations of Rhizobacteria, Mycorrhizae, and Seaweed, and Supplementary Irrigation on Growth and Yield in Wheat Cultivars
Authors: Vafa Z. Najafi , Sohrabi Y. , Sayyed R. Z. , Luh Suriani Ni , Datta Rahul
Published: April 2021

Wheat is a staple food consumed by the majority of people in the world and its production needs to be doubled to feed the growing population. On the other hand, global wheat productivity is greatly affected due to drought and low fertility of soil under arid and semi-arid regions. Application of supplementary irrigation and plant growth-promoting rhizobacteria (PGPR) has been suggested as sustainable measures to combat drought stress and to improve soil fertility and, hence, crop yield. This research was undertaken to study the effect of supplementary irrigation together with a combination of various PGPR on the growth and yield of two wheat cultivars, namely Sardari and Sirvan. The results of variance analysis (mean of squares) showed that the effect of irrigation, cultivar, and irrigation and biofertilizer and irrigation on height, spike length, seed/spike, and numbers of spikes/m2, 1000-seed weight, and grain yield were significant at 1% probability level. The effect of cultivar and irrigation interactions showed that the highest grain yield was obtained in a treatment with two additional irrigations in Sirvan cultivar (5015.0 kg/ha) and Sardari (4838.9 kg/ha) as compared to the 3598 kg/ha and 3598.3 kg/h grain yield in Sirvan and Sardari cultivars with similar treatment, but without irrigation, i.e., dryland farming. Drought conditions significantly affected the wheat grain yield while supplementary irrigation resulted in 39.38% and 34.48% higher yields in Sirvan and Sardari cultivars.
Comparison of Phenolic and Flavonoid Compound Profiles and Antioxidant and α-Glucosidase Inhibition Properties of Cultivated Soybean (Glycine max) and Wild Soybean (Glycine soja)
Authors: Chen Qianru , Wang Xianxian , Yuan Xiaolong , Shi John , Zhang Chengsheng , Yan Ning , Jing Changliang
Published: April 2021

Wild soybean (Glycine soja Sieb.et Zucc; WS) has been used as a traditional food in China for many years and contains significantly higher levels of isoflavones than cultivated soybean (Glycine max; CS), but the secondary metabolites, including flavonoids and the phenolic composition differences between them, remain unclear. The results showed that WS possessed significantly higher total phenolic and flavonoid content and exhibited better antioxidant and α-glucosidase inhibition activities as well as excellent protective effects against H2O2-induced oxidative injury in a human endothelial cell line. Through metabolomic analysis, 642 metabolites were identified, and 238 showed differential expression, with 151 upregulated and 87 downregulated. A total of 79 flavonoid compounds were identified, 42 of which were upregulated in WS. 2′-Hydroxygenistein, garbanzol, protocatechuic aldehyde, ligustilide, and resveratrol were the most discriminated compounds in WS. The metabolic pathway analysis of differential metabolites related to the biosynthesis of flavonoids and phenolic acids were the biosynthesis of phenylpropanoids, flavonoids, isoflavonoids, flavones, and flavonols. This study substantially elucidated differences in the content of flavonoids and biological activities between WS and CS, which is useful information for the effective utilization of these two black soybean species in food processing.