bluuebunny/crossref_metadata_2025 huggingface dataset:
Deep behavioral impairment in the pearl oyster Pinctada radiata exposed to anthropogenic noise and light stress
Authors: Charifi Mohcine , Khalifa Razan , Giraldes Bruno W. , Sow Mohamedou , Hizam Zainab , Carrara Mathieu , Maneux Eric , Hamza Shafeeq , Bassères Anne , Blanc Philippe , Leitão Alexandra , Massabuau Jean-Charles
Published: November 2023

The pearl oyster Pinctada radiata is an iconic species in the Arabian Gulf, which is one of the ecosystems most at risk in the world because of the multiple sources of pollution it faces. Alongside chemical pollution, the Gulf is ranked first with regard to noise and light pollution, and pearl oyster populations are at risk. The impact of these latter types of pollution on marine invertebrates is still poorly known. We used the difference in noise and brightness that can exist between a very quiet room without artificial lighting and a standard laboratory room equipped with a standard aquarium as a testbed to explore the possible impact of noise and light pollution on the behavioral and biological traits of Pinctada radiata without added chemical exposure. During an experiment that lasted 2.5 months, we analyzed their grouping behavior, valve activity, biological rhythm, growth rate and spawning activity. In the standard aquarium kept in the laboratory room, the oysters dispersed instead of regrouping as in their natural environment, regrouping which was observed in the quiet room. They stayed closed longer, the opening amplitude of their valves was systematically lower, and in the closed position, they squeezed their valves more tightly when subjected to noise and light pollution. Their daily opening rhythm was strongly structured by switching the electric light on and off, and females showed significantly less egg-laying behavior. In conclusion, seemingly innocuous human activities can lead to very significant alterations in pearl oyster behavior. We propose that it could have significant effects on populations and ecosystems.
Effect of matrix suction on the shear strength characteristics of reinforced granite residual soil
Authors: Deng Xiangwen , Zhu Zhengyu , Lin Xi yang , Yang Xue qiang
Published: November 2023

Introduction: The soil in geogrid-reinforced structures is typically unsaturated, with the shear strength provided by both the matrix suction and the reinforced body. Traditional structural designs for saturated soils only consider the shear strength provided by the reinforced body, neglecting the part provided by matrix suction. As a result, the design for reinforced structures is biased toward conservatism.

Method: The study examined the matrix suction-provided shear strength in reinforced soils through strain-controlled triaxial and soil-water characteristic curve (SWCC) pressure plate instrumentation. The feasibility of the Schrefler and Khalili unsaturated soil shear strength formulas for predicting shear strength based on matrix suction forces was verified.

Results: The study revealed that the cohesion of saturated reinforced soil exhibits a significant decrease in contrast with unsaturated reinforced soil, with matrix suction serving as a crucial consideration for reinforced structure design.

Discussion: The experimental results confirm the suitability of applying the quasi-cohesion increment theory to reinforced clays. The Khalili formula can be utilized to predict the quasi cohesion of unsaturated reinforced soils with greater accuracy under diverse dry density conditions. The results obtained using post-shear moisture content were closer to the measured values than those using initial moisture content.
Investigation into the mechanism of action of the antimicrobial peptide epilancin 15X
Authors: Wu Chunyu , Lower B. Alexis , Moreira Ryan , Dorantes Darian , Le Tung , Giurgiu Constantin , Shi Yanxiang , van der Donk Wilfred A.
Published: November 2023

Addressing the current antibiotic-resistance challenge would be aided by the identification of compounds with novel mechanisms of action. Epilancin 15X, a lantibiotic produced by Staphylococcus epidermidis 15 × 154, displays antimicrobial activity in the submicromolar range against a subset of pathogenic Gram-positive bacteria. S. epidermidis is a common member of the human skin or mucosal microbiota. We here investigated the mechanism of action of epilancin 15X. The compound is bactericidal against Staphylococcus carnosus as well as Bacillus subtilis and appears to kill these bacteria by membrane disruption. Structure–activity relationship studies using engineered analogs show that its conserved positively charged residues and dehydroamino acids are important for bioactivity, but the N-terminal lactyl group is tolerant of changes. Epilancin 15X treatment negatively affects fatty acid synthesis, RNA translation, and DNA replication and transcription without affecting cell wall biosynthesis. The compound appears localized to the surface of bacteria and is most potent in disrupting the membranes of liposomes composed of negatively charged membrane lipids in a lipid II independent manner. Epilancin 15X does not elicit a LiaRS response in B. subtilis but did upregulate VraRS in S. carnosus. Treatment of S. carnosus or B. subtilis with epilancin 15X resulted in an aggregation phenotype in microscopy experiments. Collectively these studies provide new information on epilancin 15X activity.
Co-exposure of dimethomorph and imidacloprid: effects on soil bacterial communities in vineyard soil
Authors: Chang Jean , Shen Fo-Ting , Lai Wei-An , Liao Chien-Sen , Chen Wen-Ching
Published: November 2023

In Taiwan, the pesticides dimethomorph and imidacloprid are recommended for pest control in vineyards. Therefore, tank-mixing of these two pesticides is usually a routine practice before application. This study analyzed the influence of vineyard soil microbial flora under the recommended and high dosages (100 times the recommended dosage) of dimethomorph and imidacloprid. Individual and combined applications of pesticides were also tested through batches of soil incubation experiments. Four treatments—control (C), dimethomorph (DT), imidacloprid (IM), and mixed application of dimethomorph and imidacloprid (ID)—were used in the experimental design. From the soil metabolism, no significant reaction was observed after 2 months in the recommended dosage group, regardless of whether the pesticides were being applied individually or combined. For the high dosage, imidacloprid showed a higher effect than the co-exposure treatments, showing a possible prolonged effect after its repetitive application. From PCoA analysis, pesticide treatments altered the soil ecology after 2 months, and the effect of imidacloprid can be explicitly observed at high dosages. At the phylum level,Acidobacteriacan indicate pesticide application around the recommended dosage. It was inhibited by ID on day 7 and was augmented by all pesticides on day 63. The effect of the recommended dosage of pesticide mixtures after 2 months of incubation was revealed in the minor familiesGemmataceaeandPirellulaceae, while the high dosage treatments affected both the core and the minor families. Our findings verified the changes in the composition of microbial communities upon pesticide application, which would affect carbon, nitrogen, sulfur, phosphorous cycles, and contaminant removal ability within the vineyard.
Isolation of endophytic fungi from Cotoneaster multiflorus and screening of drought-tolerant fungi and evaluation of their growth-promoting effects
Authors: Lü Zeng-Wei , Liu Hao-Yang , Wang Chen-Lu , Chen Xue , Huang Yi-Xiao , Zhang Miao-Miao , Huang Qiu-Liang , Zhang Guo-Fang
Published: November 2023

In the context of climate change and human factors, the drought problem is a particularly serious one, and environmental pollution caused by the abuse of chemical fertilizers and pesticides is increasingly serious. Endophytic fungi can be used as a protection option, which is ecologically friendly, to alleviate abiotic stresses on plants, promote plant growth, and promote the sustainable development of agriculture and forestry. Therefore, it is of great significance to screen and isolate endophytic fungi that are beneficial to crops from plants in special habitats. In this study, endophytic fungi were isolated from Cotoneaster multiflorus, and drought-tolerant endophytic fungi were screened by simulating drought stress with different concentrations of PEG-6000, and the growth-promoting effects of these drought-tolerant strains were evaluated. A total of 113 strains of endophytic fungi were isolated and purified from different tissues of C. multiflorus. After simulated drought stress, 25 endophytic fungi showed strong drought tolerance. After ITS sequence identification, they belonged to 7 genera and 12 species, including Aspergillus, Fusarium, Colletotrichum, Penicillium, Diaporthe, Geotrichum, and Metarhizium. According to the identification and drought stress results, 12 strains of endophytic fungi with better drought tolerance were selected to study their abilities of dissolving inorganic phosphorus and potassium feldspar powder and producing indole-3-acetic acid (IAA). It was found that the amount of dissolved phosphorus in 7 strains of endophytic fungi was significantly higher than that of CK, and the content of soluble phosphorus was 101.98–414.51 μg. ml−1; 6 endophytic fungi had significantly higher potassium solubilization than CK, and the content of water-soluble potassium ranged from 19.17 to 30.94 mg·l−1; 6 strains have the ability to produce IAA, and the yield of IAA ranged between 0.04 and 0.42 mg. ml−1. This study for the first time identified the existence of endophytic fungi with drought tolerance and growth-promoting function in C. multiflorus, which could provide new direction for plant drought tolerance and growth promotion fungi strain resources. It also provides a theoretical basis for the subsequent application of endophytic fungi of C. multiflorus in agricultural and forestry production to improve plant tolerance.
Effect of pH and medium composition on chain elongation with Megasphaera hexanoica producing C4-C8 fatty acids
Authors: Fernández-Blanco Carla , Veiga María C. , Kennes Christian
Published: November 2023

Introduction

Chain elongation technology, which involves fermentation with anaerobic bacteria, has gained attention for converting short and medium chain substrates into valuable and longer-chain products like medium chain fatty acids (MCFAs). In the recent past, the focus of studies with pure chain elongating cultures was on species of other genera, mainly Clostridium kluyveri. Recently, other chain elongators have been isolated that deserve further research, such as Megasphaera hexanoica.

Methods

In this study, batch studies were performed in bottles with two different media to establish the optimal conditions for growth of M. hexanoica: (a) a medium rich in different sources of nitrogen and (b) a medium whose only source of nitrogen is yeast extract. Also, batch bioreactor studies at pH values of 5.8, 6.5 and 7.2 were set up to study the fermentation of lactate (i.e., electron donor) and acetate (i.e., electron acceptor) by M. hexanoica.

Results and discussion

Batch bottle studies revealed the yeast extract (YE) containing medium as the most promising in terms of production/cost ratio, producing n-caproate rapidly up to 2.62 ± 0.24 g/L. Subsequent bioreactor experiments at pH 5.8, 6.5, and 7.2 confirmed consistent production profiles, yielding C4-C8 fatty acids. A fourth bioreactor experiment at pH 6.5 and doubling both lactate and acetate concentrations enhanced MCFA production, resulting in 3.7 g/L n-caproate and 1.5 g/L n-caprylate. H2 and CO2 production was observed in all fermentations, being especially high under the increased substrate conditions. Overall, this study provides insights into M. hexanoica’s behavior in lactate-based chain elongation and highlights optimization potential for improved productivity.
A novel candidate hepatitis C virus genotype 4 subtype identified by next generation sequencing full-genome characterization in a patient from Saudi Arabia
Authors: Di Stefano Mariantonietta , Ismail Mona H. , Leitner Thomas , Faleo Giuseppina , Alwazzeh Marwan Jabr , Mbisa Jean Lutamyo , Fiore Josè Ramon , Santantonio Teresa Antonia
Published: November 2023

Background and aim

Hepatitis C virus (HCV) infection is a major global public health concern, being a leading cause of chronic liver diseases such as chronic hepatitis, cirrhosis, and hepatocellular carcinoma. The virus is classified into 8 genotypes and 93 subtypes, each displaying distinct geographic distributions. Genotype 4 is the most predominant in the Middle East and Eastern Mediterranean and is associated with high rates of hepatitis C infection worldwide. This study used next-generation sequencing to fully characterize the HCV genome and identify a novel subtype within genotype 4 isolated from a 64-year-old Saudi man diagnosed with hepatitis C.

Methods

We analyzed the complete genome of the 141-HCV isolate using whole-genome sequencing.

Results

Our phylogenetic reconstructions, based on the entire genome of HCV-4 strains, revealed that the 141-HCV isolate formed a distinct group within the genotype 4 classification, providing valuable new insights into the variability of HCV.

Conclusion

This discovery of a previously unclassified HCV subtype within genotype 4 sheds light on the ongoing evolution and diversity of the virus. Such knowledge has significant implications for diagnostic and therapeutic approaches, as different subtypes may exhibit varying drug sensitivities and resistance profiles.
The gut microbiome dysbiosis and regulation by fecal microbiota transplantation: umbrella review
Authors: Zhang Xianzhuo , Luo Xufei , Tian Liang , Yue Ping , Li Mengyao , Liu Kefeng , Zhu Daoming , Huang Chongfei , Shi Qianling , Yang Liping , Xia Zhili , Zhao Jinyu , Ma Zelong , Li Jianlong , Leung Joseph W. , Lin Yanyan , Yuan Jinqiu , Meng Wenbo , Li Xun , Chen Yaolong
Published: November 2023

Background

Gut microbiome dysbiosis has been implicated in various gastrointestinal and extra-gastrointestinal diseases, but evidence on the efficacy and safety of fecal microbiota transplantation (FMT) for therapeutic indications remains unclear.

Methods

The gutMDisorder database was used to summarize the associations between gut microbiome dysbiosis and diseases. We performed an umbrella review of published meta-analyses to determine the evidence synthesis on the efficacy and safety of FMT in treating various diseases. Our study was registered in PROSPERO (CRD42022301226).

Results

Gut microbiome dysbiosis was associated with 117 gastrointestinal and extra-gastrointestinal. Colorectal cancer was associated with 92 dysbiosis. Dysbiosis involving Firmicutes (phylum) was associated with 34 diseases. We identified 62 published meta-analyses of FMT. FMT was found to be effective for 13 diseases, with a 95.56% cure rate (95% CI: 93.88–97.05%) for recurrent Chloridoids difficile infection (rCDI). Evidence was high quality for rCDI and moderate to high quality for ulcerative colitis and Crohn’s disease but low to very low quality for other diseases.

Conclusion

Gut microbiome dysbiosis may be implicated in numerous diseases. Substantial evidence suggests FMT improves clinical outcomes for certain indications, but evidence quality varies greatly depending on the specific indication, route of administration, frequency of instillation, fecal preparation, and donor type. This variability should inform clinical, policy, and implementation decisions regarding FMT.
Influence of symbiotic bacteria on the susceptibility of Plagiodera versicolora to Beauveria bassiana infection
Authors: Liu Mei , Ding Jinli , Lu Min
Published: November 2023

The symbiotic bacterial microbiota of insects has been shown to play essential roles in processes related to physiology, metabolism, and innate immunity. In this study, the symbiotic microbiome of Plagiodera versicolora at different developmental stages was analyzed using 16S rRNA high-throughput sequencing. The result showed that symbiotic bacteria community in P. versicolora was primarily made up of Actinobacteriota, Proteobacteria, Firmicutes, Bacteroidota, and Dependentiae. The bacterial composition among different age individuals were highly diverse, while 65 core genera were distributed in all samples which recommend core bacterial microbiome. The 8 species core bacteria were isolated from all samples, and all of them were classified as Pseudomonas sp. Among them, five species have been proven to promote the vegetable growth of Beauveria bassiana. Moreover, the virulence of B. bassiana against nonaxenic larvae exceeded B. bassiana against axenic larvae, and the introduction of the Pseudomonas sp. to axenic larvae augmented the virulence of fungi. Taken together, our study demonstrates that the symbiotic bacteria of P. versicolora are highly dissimilar, and Pseudomonas sp. core bacteria can promote host infection by entomopathogenic fungus. This result emphasizes the potential for harnessing these findings in the development of effective pest management strategies.
A novel deep learning model for glioma epilepsy associated with the identification of human cytomegalovirus infection injuries based on head MR
Authors: Wang Wei , Li Xuanyi , Ye Lou , Yin Jian
Published: November 2023

Purpose

In this study, a deep learning model was established based on head MRI to predict a crucial evaluation parameter in the assessment of injuries resulting from human cytomegalovirus infection: the occurrence of glioma-related epilepsy. The relationship between glioma and epilepsy was investigated, which serves as a significant indicator of labor force impairment.

Methods

This study enrolled 142 glioma patients, including 127 from Shengjing Hospital of China Medical University, and 15 from the Second Affiliated Hospital of Dalian Medical University. T1 and T2 sequence images of patients’ head MRIs were utilized to predict the occurrence of glioma-associated epilepsy. To validate the model’s performance, the results of machine learning and deep learning models were compared. The machine learning model employed manually annotated texture features from tumor regions for modeling. On the other hand, the deep learning model utilized fused data consisting of tumor-containing T1 and T2 sequence images for modeling.

Results

The neural network based on MobileNet_v3 performed the best, achieving an accuracy of 86.96% on the validation set and 75.89% on the test set. The performance of this neural network model significantly surpassed all the machine learning models, both on the validation and test sets.

Conclusion

In this study, we have developed a neural network utilizing head MRI, which can predict the likelihood of glioma-associated epilepsy in untreated glioma patients based on T1 and T2 sequence images. This advancement provides forensic support for the assessment of injuries related to human cytomegalovirus infection.
Use of AgomiR and AntagomiR technologies to alter satellite cell proliferation in vitro, miRNA expression, and muscle fiber hypertrophy in intrauterine growth-restricted lambs
Authors: Greene M. A. , Worley G. A. , Udoka A. N. S. , Powell R. R. , Bruce T. , Klotz J. L. , Bridges W. C. , Duckett S. K.
Published: November 2023

Introduction: microRNAs (miRNAs) are small non-coding RNAs that work at the posttranscriptional level to repress gene expression. Several miRNAs are preferentially expressed in skeletal muscle and participate in myogenesis. This research was conducted to alter endogenous miRNA expression in skeletal muscle to promote muscle hypertrophy.

Methods: Two experiments were conducted using mimic/agomiR or antagomir technologies to alter miRNA expression and examine changes in myoblast proliferation in vitro (experiment 1) and muscle hypertrophy in vivo (experiment 2). In vitro experiments found that antagomiR-22-3p and mimic-127 increased myoblast proliferation compared to other miRNA treatments or controls. These miRNA treatments, antagomiR-22-3p (ANT22) and agomiR-127 (AGO127), were then used for intramuscular injections in longissimus muscle.

Results and discussion: The use of antagomiR or mimic/agomiR treatments down-regulated or up-regulated, respectively, miRNA expression for that miRNA of interest. Expression of predicted target KIF3B mRNA for miR-127 was up-regulated and ACVR2a mRNA was up-regulated for miR-22-3p. ANT22 injection also up-regulated the major regulator of protein synthesis (mTOR). Proteomic analyses identified 11 proteins for AGO127 and 9 proteins for ANT22 that were differentially expressed. Muscle fiber type and cross-sectional area were altered for ANT22 treatments to transition fibers to a more oxidative state. The use of agomiR and antagomir technologies allows us to alter miRNA expression in vitro and in vivo to enhance myoblast proliferation and alter muscle fiber hypertrophy in IUGR lambs during early postnatal growth.
The dual-path hypothesis for the emergence of anosognosia in Alzheimer’s disease
Authors: Andrade Katia , Guieysse Thomas , Medani Takfarinas , Koechlin Etienne , Pantazis Dimitrios , Dubois Bruno
Published: November 2023

Although neurocognitive models have been proposed to explain anosognosia in Alzheimer’s disease (AD), the neural cascade responsible for its origin in the human brain remains unknown. Here, we build on a mechanistic dual-path hypothesis that brings error-monitoring and emotional processing systems as key elements for self-awareness, with distinct impacts on the emergence of anosognosia in AD. Proceeding from the notion of anosognosia as a dimensional syndrome, varying between a lack of concern about one’s own deficits (i.e., anosodiaphoria) and a complete lack of awareness of deficits, our hypothesis states that (i) unawareness of deficits would result from primary damage to the error-monitoring system, whereas (ii) anosodiaphoria would more likely result from an imbalance between emotional processing and error-monitoring. In the first case, a synaptic failure in the error-monitoring system, in which the anterior and posterior cingulate cortices play a major role, would have a negative impact on error (or deficits) awareness, preventing patients from becoming aware of their condition. In the second case, an impairment in the emotional processing system, in which the amygdala and the orbitofrontal cortex play a major role, would prevent patients from monitoring the internal milieu for relevant errors (or deficits) and assigning appropriate value to them, thus biasing their impact on the error-monitoring system. Our hypothesis stems on two scientific premises. One comes from preliminary results in AD patients showing a synaptic failure in the error-monitoring system along with a decline of awareness for cognitive difficulties at the time of diagnosis. Another comes from the somatic marker hypothesis, which proposes that emotional signals are critical to adaptive behavior. Further exploration of these premises will be of great interest to illuminate the foundations of self-awareness and improve our knowledge of the underlying paths of anosognosia in AD and other brain disorders.
Automatic classification of hyperkinetic, tonic, and tonic-clonic seizures using unsupervised clustering of video signals
Authors: Ojanen Petri , Kertész Csaba , Morales Elizabeth , Rai Pragya , Annala Kaapo , Knight Andrew , Peltola Jukka
Published: November 2023

Introduction

This study evaluated the accuracy of motion signals extracted from video monitoring data to differentiate epileptic motor seizures in patients with drug-resistant epilepsy. 3D near-infrared video was recorded by the Nelli® seizure monitoring system (Tampere, Finland).

Methods

10 patients with 130 seizures were included in the training dataset, and 17 different patients with 98 seizures formed the testing dataset. Only seizures with unequivocal hyperkinetic, tonic, and tonic-clonic semiology were included. Motion features from the catch22 feature collection extracted from video were explored to transform the patients' videos into numerical time series for clustering and visualization.

Results

Changes in feature generation provided incremental discrimination power to differentiate between hyperkinetic, tonic, and tonic-clonic seizures. Temporal motion features showed the best results in the unsupervised clustering analysis. Using these features, the system differentiated hyperkinetic, tonic and tonic-clonic seizures with 91, 88, and 45% accuracy after 100 cross-validation runs, respectively. F1-scores were 93, 90, and 37%, respectively. Overall accuracy and f1-score were 74%.

Conclusion

The selected features of motion distinguished semiological differences within epileptic seizure types, enabling seizure classification to distinct motor seizure types. Further studies are needed with a larger dataset and additional seizure types. These results indicate the potential of video-based hybrid seizure monitoring systems to facilitate seizure classification improving the algorithmic processing and thus streamlining the clinical workflow for human annotators in hybrid (algorithmic-human) seizure monitoring systems.
Routine laboratory parameters to support decision on parenteral nutrition in palliative care
Authors: Kum Lea , Zeilinger Elisabeth L. , Vohla Dagmar , Kitta Anna , Brunevskaya Nadine , Adamidis Feroniki , Ecker Franziska , Masel Eva K. , Mayr-Pirker Brigitte , Meyer Alexa L. , Sturtzel Bärbel , Kreye Gudrun , Unseld Matthias
Published: November 2023

Introduction

Parenteral nutrition (PN) is widely used in palliative care (PC), but there is limited evidence to support its use at the end of life (EOL). This aim of this was to investigate the relationship between routine laboratory parameters and survival in patients receiving PN, and to develop a decision tree model to support clinicians decide whether to start or forgo PN.

Methods

The laboratory parameters of 113 patients with advanced diseases who were admitted to a specialized palliative care unit (PCU) were analyzed at two points in time: T0 = before PN, T1 = two weeks after initiation of PN. Univariate Mann-Whitney U-tests and multivariate linear regression models, as well as a decision tree analysis were computed; all in relation to survival time.

Results

The final regression model was significant with p = 0.001 (adjusted R2 = 0.15) and included two predictors for survival time after PN initiation: the CRP/albumin ratio and urea at T1 (ps = 0.019). Decision tree analysis revealed three important predictors for classification of survival time after PN initiation: CRP, urea, and LDH (all at T0).

Discussion

The decision tree model may help to identify patients likely to benefit from PN, thus supporting the clinical decision whether or not to start PN.
Statutory health insurance-covered pre-exposure prophylaxis in Germany: changing trends in nationwide tenofovir disoproxil/emtricitabine prescriptions during the COVID-19 pandemic
Authors: Prins Henrieke , Dörre Achim , Schmidt Daniel
Published: November 2023

Background: In 2019, Germany introduced a law to reimburse high-incidence populations for pre-exposure prophylaxis (PrEP), prescribed as tenofovir-disoproxil/emtricitabine (TDF/FTC), via statutory health insurance (SHI). We studied changes in TDF/FTC-prescriptions after the implementation of this law and during the COVID-19 pandemic.

Methods: We performed an interrupted time series analysis with monthly prescriptions per defined time period as the outcome. We considered the introduction of SHI-covered PrEP (09/2019) as an interruption, and four COVID-19 waves and two national lockdowns (2020–2021) as explanatory variables. We extrapolated prescriptions had the lockdowns not occurred, and compared this to the actual prescriptions. We performed sub-analyses based on stratification by five federal states with the highest proportion of PrEP users. We assessed the models’ goodness-of-fit based on the adjusted R-squared using RStudio.

Results: The best fitting model included SHI-covered PrEP and the first COVID-19 lockdown (04/2020). The decrease in prescriptions during the first lockdown was significant nationally, and in the five federal states for single-month prescriptions. The first lockdown resulted in reductions of 57.7% (95% prediction interval (PI): 23.0%–92.4%) for single-month prescriptions, while 17.4% (95% PI: 0.28%–34.5%) nationally, and 13.9% (95% PI: -3.67%–31.5%) for 3-month prescriptions.

Conclusion: Introduction of SHI-covered PrEP resulted in a doubling of TDF/FTC-prescriptions nationwide in the first month alone. A drop in prescriptions was most apparent after the first lockdown, and particularly affected PrEP initiations, possibly due to reduced healthcare access and behavioural changes. Ongoing monitoring of TDF/FTC-prescriptions is needed to safeguard access to preventative care such as PrEP and particularly PrEP initiation during public health crises like COVID-19.
Design and test of powerful air-assisted sprayer for high stalk crops
Authors: Miao Youyi , Chen Xiao , Gong Yan , Liu Dejiang , Chen Jian , Wang Guo , Zhang Xiao
Published: November 2023

The canopies of high stalk crops, such as maize, intersect the rows at the later stages of growth, making conventional sprayers unable to enter the field for spraying. Air-assisted sprayers are often used to improve the deposition of droplets inside the canopy. In this study, the sprayer structure, the air-assisted system, and the spraying system were designed. The air-assisted conveyor system characteristics were numerically analyzed, and the wind-field distribution was tested. The wind-field distribution results showed that the near-ground wind speed exceeded 5 m s-1 in the sampling interval from 10 to 35 metres. The wind field covered a concentrated spatial area with a downward pressure trend, resulting in better drift resistance and penetration. Field tests for droplet distribution were conducted at three maize heights to verify the powerful air-assisted sprayer's technical performance and working quality. The test results showed that the droplet deposition and coverage decreased gradually along the range direction, and the top layer had the highest deposition and coverage across the canopy. The upper canopy of 0 to 12 metres range demonstrated a greater extent of coverage and deposition. The peak deposition area expanded from 9 to 33 metres in the lower canopy, with an average value of 3.77 μg cm-2. The droplet coverage within the 30 to 60 metres range only amounted to 15% to 18% of the total coverage.
Assessment of the changes in seed yield and nutritional quality of quinoa grown under rainfed Mediterranean environments
Authors: Matías Javier , Rodríguez María José , Cruz Verónica , Calvo Patricia , Granado-Rodríguez Sara , Poza-Viejo Laura , Fernández-García Nieves , Olmos Enrique , Reguera María
Published: November 2023

Climate change is considered a serious threat to agriculture and food security. It is linked to rising temperatures and water shortages, conditions that are expected to worsen in the coming decades. Consequently, the introduction of more drought-tolerant crops is required. Quinoa (Chenopodium quinoa Willd.) has received great attention worldwide due to the nutritional properties of its seeds and its tolerance to abiotic stress. In this work, the agronomic performance and seed nutritional quality of three quinoa varieties were studied during two consecutive years (2019-2020) under three water environmental conditions of Southwestern Europe (irrigated conditions, fresh rainfed, and hard rainfed) with the goal of determining the impact of rainfed conditions on this crop performance. High precipitations were recorded during the 2020 growing season resulting in similar grain yield under irrigation and fresh rainfed conditions. However, in 2019, significant yield differences with penalties under water-limiting conditions were found among the evaluated environmental conditions. Furthermore, nutritional and metabolomic differences were observed among seeds harvested from different water environments including the progressive accumulation of glycine betaine accompanied by an increase in saponin and a decrease in iron with water limitation. Generally, water-limiting environments were associated with increased protein contents and decreased yields preserving a high nutritional quality despite particular changes. Overall, this work contributes to gaining further knowledge about how water availability affects quinoa field performance, as it might impact both seed yield and quality. It also can help reevaluate rainfed agriculture, as water deficit can positively impact the nutritional quality of seeds.
Genome-wide investigation and expression analysis of OSCA gene family in response to abiotic stress in alfalfa
Authors: Li Xiaohong , Wang Xiaotong , Ma Xuxia , Cai Wenqi , Liu Yaling , Song Wenxue , Fu Bingzhe , Li Shuxia
Published: November 2023

Alfalfa is an excellent leguminous forage crop that is widely cultivated worldwide, but its yield and quality are often affected by drought and soil salinization. Hyperosmolality-gated calcium-permeable channel (OSCA) proteins are hyperosmotic calcium ion (Ca2+) receptors that play an essential role in regulating plant growth, development, and abiotic stress responses. However, no systematic analysis of the OSCA gene family has been conducted in alfalfa. In this study, a total of 14 OSCA genes were identified from the alfalfa genome and classified into three groups based on their sequence composition and phylogenetic relationships. Gene structure, conserved motifs and functional domain prediction showed that all MsOSCA genes had the same functional domain DUF221. Cis-acting element analysis showed that MsOSCA genes had many cis-regulatory elements in response to abiotic or biotic stresses and hormones. Tissue expression pattern analysis demonstrated that the MsOSCA genes had tissue-specific expression; for example, MsOSCA12 was only expressed in roots and leaves but not in stem and petiole tissues. Furthermore, RT–qPCR results indicated that the expression of MsOSCA genes was induced by abiotic stress (drought and salt) and hormones (JA, SA, and ABA). In particular, the expression levels of MsOSCA3, MsOSCA5, MsOSCA12 and MsOSCA13 were significantly increased under drought and salt stress, and MsOSCA7, MsOSCA10, MsOSCA12 and MsOSCA13 genes exhibited significant upregulation under plant hormone treatments, indicating that these genes play a positive role in drought, salt and hormone responses. Subcellular localization results showed that the MsOSCA3 protein was localized on the plasma membrane. This study provides a basis for understanding the biological information and further functional analysis of the MsOSCA gene family and provides candidate genes for stress resistance breeding in alfalfa.
Making meaning of trauma in psychosis
Authors: van Sambeek Nienke , Franssen Gaston , van Geelen Stefan , Scheepers Floortje
Published: November 2023

Background

Finding new meaning and identity in the aftermath of trauma has been identified as a key process of mental health recovery. However, research indicates that this meaning-making process is compromised in people with psychosis. Considering the high prevalence, yet under-treatment of trauma in people with psychosis, it is urgent to gain insight into how their meaning-making process can be supported.

Aim

To gain insight into how people with psychosis make meaning of trauma and identify barriers and facilitators in their meaning-making process.

Methods

Qualitative inquiry of N = 21 interviews transcripts from the Dutch Psychiatry Storybank. We included interviews of people who (a) lived through multiple psychotic episodes, and (b) spontaneously addressed traumatic experiences in a low-structured interview. Storyline analysis was performed to gain insight into the meaning-making of trauma within their self-stories. Psychosocial conceptualizations of narrative identity were used to inform the analysis. A data-validation session with four experts-by-experience was organized to check and improve the quality of our analysis.

Results

We identified four different story types: (1) Psychiatry as the wrong setting to find meaning; (2) The ongoing struggle to get trauma-therapy; (3) Exposure to trauma as a threat to a stable life, and (4) Disclosure as the key to resolving alienation. Each story type comprises a different plot, meaning of trauma withing the self-story, (lack of) integration and barriers and facilitators in the meaning-making process. Overall, barriers in the meaning-making process were mostly situated within mental healthcare and stigma-related. People felt particularly hindered by pessimistic ideas on their capacity to develop self-insight and cope with distress, resulting in limited treatment options. Their process of adaptive meaning-making often started with supportive, non-judgmental relationships with individuals or communities that offered them the safety to disclose trauma and motivated them to engage in a process of self-inquiry and growth.

Conclusion

The outcomes illuminate the social context of the meaning-making challenges that people with psychosis face and illustrate the devastating influence of stigma. Our outcomes offer guidance to remove barriers to adaptive meaning-making in people with psychosis, and can help clinicians to attune to differences in the meaning-making of trauma.
Protocol of a study to benchmark occupational health and safety in Japan: W2S-Ohpm study
Authors: Nagata Tomohisa , Odagami Kiminori , Nagata Masako , Adi Nuri Purwito , Mori Koji
Published: November 2023

We aim to conduct a prospective cohort study to benchmark occupational health and safety in Japan. Here, we describe the detailed protocol for the baseline survey based on the Checklist for Reporting Results of Internet E-Surveys. We conducted the baseline survey for the prospective cohort study in 2022. Our target population was workers in Japan aged 20 years or older, who we sampled to be representative of the Japanese workforce, stratified by sex, age, and region. Among 59,272 registered monitors who answered the initial screening questions, 29,997 completed the survey. After excluding 2,304 invalid responses, we used 27,693 valid participants in our final analysis. The number and mean age of men were 15,201 (55%) and 46 years; those of women were 12,492 (45%) and 45 years. With respect to sex, age, and regional composition, our sampling was representative of Japan’s working population. Our sampling for employment status and industry yielded almost the same proportions as a government-led representative sampling of workers in Japan.
Does frequent tea consumption provide any benefit to cognitive function in older adults? Evidence from a national survey from China in 2018
Authors: Wei Chen , Zhang Jiao , Chen Na , Xu Zhou , Tang Huang
Published: November 2023

Objectives

This present study aims to investigate the effect of tea consumption on cognitive function and examine possible psychosocial mechanisms in older adults.

Participants and methods

The data of this study came from the 2018 wave of the Chinese Longitudinal Healthy Longevity Survey(CLHLS), and a total of 11,910 valid samples were included. We used ordinary least squares (OLS) to explore whether frequent tea consumption had significant effect on the cognitive function of older people. The problem of endogeneity was addressed by using a propensity score matching (PSM). Then we further explored the psychosocial mechanisms of the effect using a stepwise regression approach.

Results

Frequent tea consumption produced a positive effect on Mini-Mental State Examination (MMSE) score (coefficient = 0.340, p < 0.01), and PSM showed similar results. Specifically, the positive effect of green tea (coefficient 0.409, p < 0.01) was significantly greater than the other teas (coefficient 0.261, p < 0.1). Moreover, frequent tea drinkers were 59.7, 74.8, and 81.8% less likely to have severe, moderate and mild cognitive impairment respectively, compared to infrequent tea drinkers (p < 0.01). Levels of depression and sleep quality had partial mediation effect for frequent tea consumption on cognitive function, accounting for 27.6 and 3.5% of the total effect, respectively.

Conclusion

Frequent tea consumption was found to have beneficial effects on cognitive function, especially in older people with green tea intake. Sleep quality and levels of depression partially mediated the association between frequent tea consumption and cognitive function among Chinese older adults.
Turning the promise of multipurpose prevention technologies into a market reality: a commentary
Authors: Dam Anita , Schueller Jane , Peine Kevin J. , Mason Jennifer , Dorward Emily , Vij Ashley
Published: November 2023

The promise of multipurpose prevention technologies (MPTs) for the prevention of HIV and unintended pregnancy are on the horizon. While many are still in clinical development, others are closer to becoming a realistic, accessible option for users, like the dual prevention pill (DPP). Researchers, governments, donors, and implementers will have to collaboratively address systemic challenges to successfully introduce and scale-up MPTs. To ensure the rollout of MPTs is successful, the global community should address user and country-specific needs, coordinate with advocates and policymakers, and set a realistic plan for product introduction and scale-up that considers the needs of both family planning (FP) and HIV programs, while laying the groundwork for future new product introduction. To achieve these aims, global and regional stakeholder coordination should emphasize country-led, person-centered decision-making while addressing: (1) procurement and supply chain barriers; (2) the potential burden on health systems; and (3) the impact on current programs.
The effect of the silvopastoral system on milk production and reproductive performance of dairy cows and its contribution to adaptation to a changing climate in the drylands of Benin (West-Africa)
Authors: Assani Seidou Alassan , Offoumon Oyéniran Thierry La Fronde , Sanni Worogo Sorebou Hilaire , Houaga Isidore , Koara Yarou Amoudawenou , Azalou Maximilien , Adambi Boukari Foukpe Zhairath , Idrissou Yaya , Houinato Marcel , Alkoiret Traoré Ibrahim
Published: November 2023

Knowledge about dairy cows raised in small-scale agroforestry systems in dryland areas is of paramount importance to inform policy and decision making in the dairy production sector in the current context of climate change. The aim of this study was to evaluate the effect of integrated farming systems on daily milk yield and demographic traits of dairy cows in drylands. A study conducted on 447 dairy cows was carried out to compare their milk yield and demographic parameters under different small-scale agroforestry systems in drylands of Benin: traditional silvopasture (TSS); Improved silvopasture (ISS); Small Integrated Agrosilvopasture (SIAS) and Large Integrated Agrosilvopasture (LIAS). The type of cattle farms had a significant effect (p < 0.05) on daily milk yield and demographic traits. Dairy cattle from ISS farms had the highest daily milk yield regardless of the type of dairy cow breed. Demographic traits of herds were significantly (p < 0.05) influenced by the type of dairy cattle farms. The proportion of lactating cows was higher (p < 0.05) in herds of ISS (48.4%) followed by those in TSS and LIAS (36.1 and 25.0% respectively) while SIAS was the lowest in (14.4%). The pre-weaning mortality rate was higher (p < 0.05) in TSS and LIAS farms (18.3 and 17.6 % respectively) compared to SIAS and ISS farms (5.20 and 4.60 % respectively). The fertility rate was higher (p < 0.05) in ISS and SIAS farms (92.3 and 89.6% respectively) compared to TSS and LIAS farms (68.3 and 74.2% respectively). The weaning productivity was higher (p < 0.05) in ISS and SIAS (88.6 and 85.8 % respectively) than in TSS and LIAS farms (66.1 and 67.6 % respectively). This study showed that ISS farms are characterized by higher milk yield and demographic parameters. ISS systems can then be promoted in smallholder cattle farming to improve milk production and reproductive performance of dairy cows in drylands.
Global profiling of the proteome, phosphoproteome, and N-glycoproteome of protoscoleces and adult worms of Echinococcus granulosus
Authors: Wang Zhengrong , Jia Xinyue , Ma Jing , Zhang Yanyan , Sun Yan , Bo Xinwen
Published: November 2023

Introduction

Cystic echinococcosis (CE) is a chronic zoonosis caused by infection with the metacestode of the Echinococcus granulosus. A unique characteristic of E. granulosus protoscolex (PSC) is their ability to develop bidirectionally into an adult worm in the definitive host or a secondary hydatid cyst in the intermediate host. Furthermore, cestodes have a complex life cycle involving different developmental stages; however, the mechanisms underlying this development remain unknown. Several studies have demonstrated that certain matrix proteins undergo posttranslational modifications (PTMs), including phosphorylation and glycosylation, which have important regulatory effects on their functional properties.

Methods

Systematic analyses of the proteome, phosphorylated modified proteome, and glycosylated modified proteome of protoscoleces (PSCs) and adult worms were performed using a proteomic strategy. Data are available via ProteomeXchange with identifier PXD043166.

Results

In total, 6,407 phosphorylation sites and 1757 proteins were quantified. Of these, 2032 phosphorylation sites and 770 proteins were upregulated, and 2,993 phosphorylation sites and 1,217 proteins were downregulated in adult worms compared to PSCs. A total of 612 N-glycosylation sites were identified in the 392 N-glycoproteins. Of these, 355 N-glycosylation sites and 212 N-glycoproteins were quantified. Of these, 90 N-glycosylation sites and 64 N-glycoproteins were upregulated, and 171 N-glycosylation sites and 126 N-glycoproteins were downregulated in adult worms compared to PSCs. GO enrichment analysis indicated that the differentially expressed phosphoproteins were mainly enriched in the regulation of oxidoreduction coenzyme metabolic processes, myelin sheath, and RNA helicase activity, whereas the differentially expressed N-glycoproteins were enriched in the cellular response to unfolded proteins, endoplasmic reticulum lumen, and nucleic acid binding. KEGG enrichment analysis indicated that the differently expressed phosphoproteins were mainly enriched in RNA transport, hypertrophic cardiomyopathy (HCM), glycolysis/gluconeogenesis, HIF-1 signaling pathway and pyruvate metabolism. Differentially expressed N-glycoproteins were enriched in the PI3K-Akt signaling pathway, ECM-receptor interactions, and protein processing in the endoplasmic reticulum.

Discussion

To our knowledge, this study is the first global phosphoproteomic and N-glycoproteomic analysis of E. granulosus, which provides valuable information on the expression characteristics of E. granulosus and provides a new perspective to elucidate the role of protein phosphorylation and N-glycosylation in the development of E. granulosus.