bluuebunny/crossref_metadata_2025 huggingface dataset:
Degradation of Host Proteins and Apoptosis Induced by Foot-and-Mouth Disease Virus 3C Protease
Authors: Yi Jiamin , Peng Jiangling , Ren Jingjing , Zhu Guoqiang , Ru Yi , Tian Hong , Li Dan , Zheng Haixue
Published: December 2021

Foot-and-mouth disease (FMD), induced by the foot-and-mouth disease virus (FMDV), is a highly contagious disease of cloven-hoofed animals. Previous studies have reported that FMDV 3C protease could degrade multiple host proteins; however, the degradation mechanism mediated by FMDV 3C is still unclear. Here, we found that transient expression of FMDV 3C degraded various molecules in NF-κB signaling in a dose-dependent manner, and the proteolytic activity of FMDV 3C is important for inducing degradation. Additionally, 3C-overexpression was associated with the induction of apoptosis. In this study, we showed that an apoptosis inhibitor CrmA abolished the ability of 3C to degrade molecules in NF-κB signaling. Further experiments using specific caspase inhibitors confirmed the irrelevance of caspase3, caspase8, and caspase9 activity for degradation induced by 3C. Altogether, these results suggest that FMDV 3C induces the widespread degradation of host proteins through its proteolytic activity and that the apoptosis pathway might be an important strategy to mediate this process. Further exploration of the relationship between apoptosis and degradation induced by 3C could provide novel insights into the pathogenic mechanisms of FMDV.
Parasites, Bacteria, and Associated Pathological Changes in the Digestive System of Diurnal and Nocturnal Raptors in Central Italy
Authors: Rossi Giacomo , Terracciano Giuliana , Gherardi Riccardo , Galosi Livio , Perrucci Stefania
Published: December 2021

The knowledge of raptor pathogens and associated lesions may be extremely important to enhancing raptor conservation efforts and reducing pathogen spillover to humans and domesticated animals and vice versa. Parasite infections of the digestive system and associated bacteria and pathological changes were evaluated in deceased diurnal and nocturnal raptors in central Italy. Overall, the prevalence of parasites (nematodes, cestodes, trematodes, acanthocephalans, and protozoa) identified in the examined birds was 72.41%, and most of the positive raptors (71.42%) showed multiple parasite infections. Among bacteria, Salmonella typhimurium, Salmonella enterica subspecies diarizonae, Escherichia coli, Clostridium perfringens, Yersinia enterocolitica, and Pasteurella multocida were identified. The results obtained showed that both parasites and bacteria may cause severe lesions in the digestive system of diurnal and nocturnal raptors; parasites and bacteria may concur in causing these lesions; most severe lesions are caused by the interaction of multiple pathogens, both parasites and bacteria; and the same pathogen taxa are frequently associated with the same pathological changes. This study is the first report of S. typhimurium and S. enterica subspecies diarizonae in Buteo buteo, while Andracantha mergi, Spirocerca spp., Sarcocystis dispersa, Sarcocystis columbae, and Eumonospora spp. were recorded for the first time in Italy.
Provision of Decentralized TB Care Services: A Detect–Treat–Prevent Strategy for Children and Adolescents Affected by TB
Authors: Zawedde-Muyanja Stella , Reuter Anja , Tovar Marco A. , Hussain Hamidah , Loando Mboyo Aime , Detjen Anne K. , Yuen Courtney M.
Published: December 2021

In this review, we discuss considerations and successful models for providing decentralized diagnosis, treatment, and prevention services for children and adolescents. Key approaches to building decentralized capacity for childhood TB diagnosis in primary care facilities include provider training and increased access to child-focused diagnostic tools and techniques. Treatment of TB disease should be managed close to where patients live; pediatric formulations of both first- and second-line drugs should be widely available; and any hospitalization should be for as brief a period as medically indicated. TB preventive treatment for child and adolescent contacts must be greatly expanded, which will require home visits to identify contacts, building capacity to rule out TB, and adoption of shorter preventive regimens. Decentralization of TB services should involve the private sector, with collaborations outside the TB program in order to reach children and adolescents where they first enter the health care system. The impact of decentralization will be maximized if programs are family-centered and designed around responding to the needs of children and adolescents affected by TB, as well as their families.
Dose Administration Aid Service in Community Pharmacies: Characterization and Impact Assessment
Authors: Vicente André , Mónico Beatriz , Lourenço Mónica , Lourenço Olga
Published: December 2021

Adherence to therapies is a primary determinant of treatment success. Lack of medication adherence is often associated with medical and psychosocial issues due to complications from underlying conditions and is an enormous waste of medical resources. Dose Administration Aid Service (DAAS) can be seen as part of the solution, allowing individual medicine doses to be organized according to the dosing schedule determined by the patient’s prescriber. The most recent systematic reviews admit the possibility of a positive impact of this service. In line with this background, the study reported in this paper aimed to characterize DAAS implementation in Portugal and understand the perceptions of pharmacists and owners of community pharmacies regarding the impact of DAAS, preferred methodology types, and State contribution. The study was guided by qualitative description methodology and reported using the consolidated criteria for reporting qualitative research (COREQ) checklist. Data were collected through semi-structured interviews with 18 pharmacists and/or owners of community pharmacies. Using qualitative content analysis, we identified categories that revealed that automated weekly methodology is the preferred methodology, because of its easiness of use and lower cost of preparation. However, the investment cost was felt to be too high by the participants considering the number of potential users for implementation in practice. Participants were also unanimous in recognizing that DAAS has a very positive impact in terms of safety and medication adherence, and the majority agreed that it also helped reduce medication waste. Implications of these findings for medication adherence are discussed.
Does Lewis’ Theory of Causation Permit Time Travel?
Authors: Dowe Phil
Published: December 2021

David Lewis aimed to give an account of causation, and in particular, a semantics for the counterfactuals to which his account appeals, that is compatible with backwards causation and time travel. I will argue that he failed, but not for the reasons that have been offered to date, specifically by Collins, Hall and Paul and by Wasserman. This is significant not the least because Lewis’ theory of causation was the most influential theory over the last quarter of the 20th century; and moreover, Lewis’ spirited defence of time travel in the 1970s has shaped philosophers’ approach to time travel to this day.
Aristotle’s Political Justice and the Golden Ratio between the Three Opposing Criteria for the Distribution of Public Goods among Citizens: Freedom, Wealth and Virtue
Authors: Salamone Maria Antonietta
Published: December 2021

In this article, I interpret Book V of the Nicomachean Ethics in which Aristotle presents a geometrical problem to explain which is the Best Criterion for the Distribution of Political and Economic Rights and Duties among Citizens, starting from the empirical evidence that there are three opposing opinions on which is the fairest distribution criterion: for some it is Freedom (Democrats), for others Wealth (Oligarchs), and for others Virtue (Aristocrats). Against the almost unique and most quoted interpretation of the geometrical problem, I present my mathematical solution, which I arrived at thanks to the Doctrine of the Four Causes and the Theory of the Mean. My thesis is that the Mean Term of Distributive Justice is the Golden Ratio between the opposite criteria of distribution, and the unjust distribution is the one that violates this ratio. This solution allows us to understand what is the Rational Principle at the basis of just distribution: that is, Geometrical Equality as opposed to Arithmetical Equality. Indeed, by applying the geometric figure of the Golden Triangle to the different political constitutions, I show, in line with Politics, that the Best Form of Government is the Aristocratic Politeia, i.e., a mixture of Democracy, Oligarchy and Aristocracy.
Remazol Black Decontamination Study Using a Novel One-Pot Synthesized S and Co Co-Doped TiO2 Photocatalyst
Authors: Dwiyanna Riska , Roto Roto , Wahyuni Endang Tri
Published: December 2021

This study investigated the decolorization of Remazol Black (RBB) using a TiO2 photocatalyst modified by S and Co co-doped TiO2 (S-Co-TiO2) from a single precursor. X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and UV–Vis specular reflectance spectroscopy were used to characterize the photocatalysts. The results revealed that the band-gap energy of the doped and co-doped TiO2 decreased, with the S-Co-TiO2 8% showing the greatest one, and was found to be 2.78 eV while undoped TiO2 was 3.20 eV. The presence of S and Co was also identified through SEM-EDX. An activity study on RBB removal revealed that the S-Co-TiO2 photocatalyst showed the best result compared to undoped TiO2, S-TiO2, and Co-TiO2. The S-Co-TiO2 8% photocatalyst reduced RBB concentration (20 mg L−1) up to 96% after 90 min of visible light irradiation, whereas S-TiO2, Co-TiO2, and undoped TiO2 reduced it to 89%, 56%, and 39%, respectively. A pH optimization study showed that the optimum pH of RBB decolorization by S-Co-TiO2 was 3.0, the optimum mass was 0.6 g L−1, and reuse studies show that S-Co-TiO2 8% has the potential to be used repeatedly to remove colored pollutants. The results obtained indicate that the modification of S, Co co-doped titania synthesized using a single precursor has been successfully carried out and showed excellent characteristics and activity compared to undoped or doped TiO2.
2.58 kW Narrow Linewidth Fiber Laser Based on a Compact Structure with a Chirped and Tilted Fiber Bragg Grating for Raman Suppression
Authors: Tian Xin , Gao Chenhui , Wang Chongwei , Zhao Xiaofan , Wang Meng , Xi Xiaoming , Wang Zefeng
Published: December 2021

We report a high power, narrow linewidth fiber laser based on oscillator one-stage power amplification configuration. A fiber oscillator with a center wavelength of 1080 nm is used as the seed, which is based on a high reflection fiber Bragg grating (FBG) and an output coupling FBG of narrow reflection bandwidth. The amplifier stage adopted counter pumping. By optimizing the seed and amplifier properties, an output laser power of 2276 W was obtained with a slope efficiency of 80.3%, a 3 dB linewidth of 0.54 nm and a signal to Raman ratio of 32 dB, however, the transverse mode instability (TMI) began to occur. For further increasing the laser power, a high-power chirped and tilted FBG (CTFBG) was inserted between the backward combiner and the output passive fiber, experimental results showed that both the threshold of Stimulated Raman scattering (SRS) and TMI increased. The maximum laser power was improved to 2576 W with a signal to Raman ratio of 42 dB, a slope efficiency of 77.1%, and a 3 dB linewidth of 0.87 nm. No TMI was observed and the beam quality factor M2 maintained about 1.6. This work could provide a useful reference for obtaining narrow-linewidth high-power fiber lasers with high signal to Raman ratio.
Quantum Optical Experiments Modeled by Long Short-Term Memory
Authors: Adler Thomas , Erhard Manuel , Krenn Mario , Brandstetter Johannes , Kofler Johannes , Hochreiter Sepp
Published: December 2021

We demonstrate how machine learning is able to model experiments in quantum physics. Quantum entanglement is a cornerstone for upcoming quantum technologies, such as quantum computation and quantum cryptography. Of particular interest are complex quantum states with more than two particles and a large number of entangled quantum levels. Given such a multiparticle high-dimensional quantum state, it is usually impossible to reconstruct an experimental setup that produces it. To search for interesting experiments, one thus has to randomly create millions of setups on a computer and calculate the respective output states. In this work, we show that machine learning models can provide significant improvement over random search. We demonstrate that a long short-term memory (LSTM) neural network can successfully learn to model quantum experiments by correctly predicting output state characteristics for given setups without the necessity of computing the states themselves. This approach not only allows for faster search, but is also an essential step towards the automated design of multiparticle high-dimensional quantum experiments using generative machine learning models.
Simulated Annealing Applied to HIO Method for Phase Retrieval
Authors: Zhang Yicheng , Sun Mingjie
Published: December 2021

Phase retrieval utilizing Fourier amplitudes plays a significant role in image recovery. Iterative phase retrieval algorithms have been developed to retrieve phase information that cannot be recorded by detectors directly. However, iterative algorithms face the problem of being trapped in local minima due to the nonconvexity of phase retrieval, and most existing works addressed this by optimizing in multiple runs parallelly to improve the possibility that one of these could reach the global minimum. Alternatively, we propose in this work to increase the probability of reaching the global minimum with one arbitrary initial distribution by adapting simulated annealing in the standard hybrid input-output (HIO) algorithm. Numerical and experimental results demonstrate that the proposed method reconstructs images with mean square errors 50.12% smaller than those reconstructed by HIO. More importantly, the proposed method can be applied to any HIO-based algorithm with multiple runs to further improve the performance.
Optical Gain in Commercial Laser Diodes
Authors: Vanzi Massimo
Published: December 2021

Optical gain and optical losses are separately measured in commercial laser diodes by simple analysis of spectral and electrical characteristics, and with no special specimen preparation or handling. The aim is to bring device analysis, for characterization and reliability purposes, closer to the intimate physical processes that rule over laser diode operation. Investigation includes resonating and non-resonating optical cavities.
LEO Satellites Constellation-to-Ground QKD Links: Greek Quantum Communication Infrastructure Paradigm
Authors: Ntanos Argiris , Lyras Nikolaos K. , Zavitsanos Dimitris , Giannoulis Giannis , Panagopoulos Athanasios D. , Avramopoulos Hercules
Published: December 2021

Quantum key distribution (QKD) has gained a lot of attention over the past few years, but the implementation of quantum security applications is still challenging to accomplish with the current technology. Towards a global-scale quantum-secured network, satellite communications seem to be a promising candidate to successfully support the quantum communication infrastructure (QCI) by delivering quantum keys to optical ground terminals. In this research, we examined the feasibility of satellite-to-ground QKD under daylight and nighttime conditions using the decoy-state BB84 QKD protocol. We evaluated its performance on a hypothetical constellation with 10 satellites in sun-synchronous Low Earth Orbit (LEO) that are assumed to communicate over a period of one year with three optical ground stations (OGSs) located in Greece. By taking into account the atmospheric effects of turbulence as well as the background solar radiance, we showed that positive normalized secure key rates (SKRs) up to 3.9×10−4 (bps/pulse) can be obtained, which implies that satellite-to-ground QKD can be feasible for various conditions, under realistic assumptions in an existing infrastructure.
A Shock-in-Jet Synchrotron Mirror Model for Blazars
Authors: Böttcher Markus
Published: December 2021

Reinhard Schlickeiser has made groundbreaking contributions to various aspects of blazar physics, including diffusive shock acceleration, the theory of synchrotron radiation, the production of gamma-rays through Compton scattering in various astrophysical sources, etc. This paper, describing the development of a self-consistent shock-in-jet model for blazars with a synchrotron mirror feature, is therefore an appropriate contribution to a Special Issue in honor of Reinhard Schlickeiser’s 70th birthday. The model is based on our previous development of a self-consistent shock-in-jet model with relativistic thermal and non-thermal particle distributions evaluated via Monte-Carlo simulations of diffusive shock acceleration, and time-dependent radiative transport. This model has been very successful in modeling spectral variability patterns of several blazars, but has difficulties describing orphan flares, i.e., high-energy flares without a significant counterpart in the low-frequency (synchrotron) radiation component. As a solution, this paper investigates the possibility of a synchrotron mirror component within the shock-in-jet model. It is demonstrated that orphan flares result naturally in this scenario. The model’s applicability to a recently observed orphan gamma-ray flare in the blazar 3C279 is discussed and it is found that only orphan flares with mild (≲ a factor of 2–3) enhancements of the Compton dominance can be reproduced in a synchrotron-mirror scenario, if no additional parameter changes are invoked.
On the Difference between the Radii of Gluons and Quarks
Authors: Trevisan Luis Augusto , Mirez Carlos , da Silva Djalma Inacio
Published: December 2021

In this paper, in the scope of a non-extensive statistical model for the nucleon’s structure function, the volume of the gluons in the nucleons and the relations among the temperature, T, the parameter “q” of Tsallis statistics, and the scattering energies, Q2, are studied. A system of equations with the usual sum rules are solved for the valence quarks, the experimental results for the polarized structure function, and the estimated carried moments for gluons and quarks. Each state of T and q leads to a set of chemical potentials and different radii for gluons and quarks. We conclude that gluons must occupy a larger volume than the quarks to fit the fraction of the total momentum. A linear function of the temperature with Q2 is obtained as an approach. The obtained range of temperatures is different from the previous models.
Construction of Multiple Guide RNAs in CRISPR/Cas9 Vector Using Stepwise or Simultaneous Golden Gate Cloning: Case Study for Targeting the FAD2 and FATB Multigene in Soybean
Authors: Kim Won-Nyeong , Kim Hye-Jeong , Chung Young-Soo , Kim Hyun-Uk
Published: December 2021

CRISPR/Cas9 is a commonly used technique in reverse-genetics research to knock out a gene of interest. However, when targeting a multigene family or multiple genes, it is necessary to construct a vector with multiple single guide RNAs (sgRNAs) that can navigate the Cas9 protein to the target site. In this protocol, the Golden Gate cloning method was used to generate multiple sgRNAs in the Cas9 vector. The vectors used were pHEE401E_UBQ_Bar and pBAtC_tRNA, which employ a one-promoter/one-sgRNA and a polycistronic-tRNA-gRNA strategy, respectively. Golden Gate cloning was performed with type IIS restriction enzymes to generate gRNA polymers for vector inserts. Four sgRNAs containing the pHEE401E_UBQ_Bar vector and four to six sgRNAs containing the pBAtC_tRNA vector were constructed. In practice, we constructed multiple sgRNAs targeting multiple genes of FAD2 and FATB in soybean using this protocol. These three vectors were transformed into soybeans using the Agrobacterium-mediated method. Using deep sequencing, we confirmed that the T0 generation transgenic soybean was edited at various indel ratios in the predicted target regions of the FAD2 and FATB multigenes. This protocol is a specific guide that allows researchers to easily follow the cloning of multiple sgRNAs into commonly used CRISPR/Cas9 vectors for plants.
Glucosinolates and Isothiocyantes in Processed Rapeseed Determined by HPLC-DAD-qTOF
Authors: Miklavčič Višnjevec Ana , Tamayo Tenorio Angelica , Steenkjær Hastrup Anne Christine , Hansen Natanya Majbritt Louie , Peeters Kelly , Schwarzkopf Matthew
Published: December 2021

Glucosinolates are well known as natural antimicrobials and anticarcinogenic agents. However, these compounds can lose their properties and transform into antinutrients, depending on processing conditions. In addition, the bitterness of some glucosinolate in rapeseed meal can affect the likability of the final product. Therefore, it is important to identify and determine each glucosinolate and its derived form, not just the total glucosinolate content, in order to evaluate the potential of the final rapeseed protein product. This study provides a comprehensive report of the types and quantities of glucosinolates and their derived forms (isothiocyanates) associated with different rapeseed processing conditions. Glucosinolates and isothiocyanates were determined by HPLC-DAD-qTOF. In our study, the enzymatic degradation of glucosinolates by myrosinase was the main factor affecting either glucosinolate or isothiocyanate content. Other factors such as pH seemed to influence the concentration and the presence of glucosinolates. In addition, process parameters, such as extraction time and separation technology, seemed to affect the amount and type of isothiocyanates in the final protein extracts. Overall, both determined intact glucosinolates and their derived forms of isothiocyanates can give different types of biological effects. More studies should be performed to evaluate the impact of glucosinolates and isothiocyanates on human health.
Gymnosperm Resprouting—A Review
Authors: Burrows Geoffrey E.
Published: December 2021

Gymnosperms are generally regarded as poor resprouters, especially when compared to angiosperms and particularly following major disturbance. However, is it this clear-cut? This review investigates two main aspects of gymnosperm resprouting: (i) various papers have provided exceptions to the above generalization—how frequent are these exceptions and are there any taxonomic trends?; and (ii) assuming gymnosperms are poor resprouters are there any anatomical or physiological reasons why this is the case? Five of six non-coniferous gymnosperm genera and 24 of 80 conifer genera had at least one species with a well-developed resprouting capability. This was a wider range than would be expected from the usual observation ‘gymnosperms are poor resprouters’. All conifer families had at least three resprouting genera, except the monospecific Sciadopityaceae. Apart from the aboveground stem, buds were also recorded arising from more specialised structures (e.g., lignotubers, tubers, burls and underground stems). In some larger genera it appeared that only a relatively small proportion of species were resprouters and often only when young. The poor resprouting performance of mature plants may stem from a high proportion of apparently ‘blank’ leaf axils. Axillary meristems have been recorded in a wide range of conifer species, but they often did not form an apical dome, leaf primordia or vascular connections. Buds or meristems that did form often abscised at an early stage. While this review has confirmed that conifers do not resprout to the same degree as angiosperms, it was found that a wide diversity of gymnosperm genera can recover vegetatively after substantial disturbance. Further structural studies are needed, especially of: (i) apparently blank leaf axils and the initial development of axillary meristems; (ii) specialised regeneration structures; and (iii) why high variability can occur in the resprouting capacity within species of a single genus and within genera of the same family.
Adaptogenic Properties of a Phytoecdysteroid-Rich Extract from the Leaves of Spinacia oleracea L.
Authors: Sidorova Yuliya S. , Shipelin Vladimir A. , Petrov Nikita A. , Zorin Sergey N. , Mazo Vladimir K.
Published: December 2021

Increasing the ability of the human body to adapt in conditions of physical or emotional stress is promising from the standpoint of the use of preventive nutrition containing functional food ingredients (FFI) with proven effectiveness in complex physiological in vivo studies. In this work, we developed FFI from spinach leaves (Spinacia oleracea L.) with a high content of polyphenols and adaptogens—phytoecdysteroids. Using in vivo models of increased physical activity and immobilization-induced emotional stress, we evaluated the nonspecific resistance of rats in response to the addition of the developed FFI to the diet. In the acute toxicity experiment, we found no signs of FFI toxicity up to 5000 mg/kg body weight. As a result of the daily 26-day consumption of FFI, we observed an anxiolytic effect in physiological studies. FFI prevented an increase in the content of biogenic amines in the blood, the main markers of the stress system, and had a positive effect on the lipid metabolism of the rats. The obtained results demonstrate a “smoothing” effect on the body’s reaction in response to induced stress conditions.
The Role of DNA Topoisomerase Binding Protein 1 (TopBP1) in Genome Stability in Arabidopsis
Authors: Parra-Nunez Pablo , Cooper Claire , Sanchez-Moran Eugenio
Published: December 2021

DNA topoisomerase II (TOPII) plays a very important role in DNA topology and in different biological processes such as DNA replication, transcription, repair, and chromosome condensation in higher eukaryotes. TOPII has been found to interact directly with a protein called topoisomerase II binding protein 1 (TopBP1) which also seems to have important roles in DNA replication and repair. In this study, we conducted different experiments to assess the roles of TopBP1 in DNA repair, mitosis, and meiosis, exploring the relationship between TOPII activity and TopBP1. We found that topbp1 mutant seedlings of Arabidopsis thaliana were hypersensitive to cisplatin treatment and the inhibition of TOPII with etoposide produced similar hypersensitivity levels. Furthermore, we recognised that there were no significant differences between the WT and topbp1 seedlings treated with cisplatin and etoposide together, suggesting that the hypersensitivity to cisplatin in the topbp1 mutant could be related to the functional interaction between TOPII and TopBP1. Somatic and meiotic anaphase bridges appeared in the topbp1 mutant at similar frequencies to those when TOPII was inhibited with merbarone, etoposide, or ICFR-187. The effects on meiosis of TOPII inhibition were produced at S phase/G2 stage, suggesting that catenanes could be produced at the onset of meiosis. Thus, if the processing of the catenanes is impaired, some anaphase bridges can be formed. Also, the appearance of anaphase bridges at first and second division is discussed.
Molecular Manipulation of the miR396 and miR399 Expression Modules Alters the Response of Arabidopsis thaliana to Phosphate Stress
Authors: Pegler Joseph L. , Nguyen Duc Quan , Oultram Jackson M. J. , Grof Christopher P. L. , Eamens Andrew L.
Published: December 2021

In plant cells, the molecular and metabolic processes of nucleic acid synthesis, phospholipid production, coenzyme activation and the generation of the vast amount of chemical energy required to drive these processes relies on an adequate supply of the essential macronutrient, phosphorous (P). The requirement of an appropriate level of P in plant cells is evidenced by the intricately linked molecular mechanisms of P sensing, signaling and transport. One such mechanism is the posttranscriptional regulation of the P response pathway by the highly conserved plant microRNA (miRNA), miR399. In addition to miR399, numerous other plant miRNAs are also required to respond to environmental stress, including miR396. Here, we exposed Arabidopsis thaliana (Arabidopsis) transformant lines which harbor molecular modifications to the miR396 and miR399 expression modules to phosphate (PO4) starvation. We show that molecular alteration of either miR396 or miR399 abundance afforded the Arabidopsis transformant lines different degrees of tolerance to PO4 starvation. Furthermore, RT-qPCR assessment of PO4-starved miR396 and miR399 transformants revealed that the tolerance displayed by these plant lines to this form of abiotic stress most likely stemmed from the altered expression of the target genes of these two miRNAs. Therefore, this study forms an early step towards the future development of molecularly modified plant lines which possess a degree of tolerance to growth in a PO4 deficient environment.
Soybean Callus—A Potential Source of Tocopherols
Authors: Mureșan Liliana , Clapa Doina , Rusu Teodor , Wang Thomas T. Y. , Park Jae B.
Published: December 2021

In vitro cultures have been used as an effective means to achieve a high level of secondary metabolites in various plants, including soy. In this study, the contents of α-, γ-, and δ- tocopherol were quantified in soybean callus, and their amounts were compared to those of soybeans cultivated using the conventional tillage system with three weed controls (respectively without herbicide and with two variants of herbicide). Soybean callus was produced using Murashige and Skoog 1962 (MS) medium supplemented with 0.1 mg/L 6-Benzylaminopurine (BAP) and 0. 1 mg/L Thidiazuron (TDZ). The highest amount of fresh callus was obtained from soybeans from the conventional tillage system with second weed control (S-metolachlor 960 g/L, imazamox 40 g/L, and propaquizafop 100 g/L) respectively 13,652.4 ± 1177.62 mg. The analyzed tocopherols were in much higher content in soy dry callus than the soybean seeds (5.63 µg/g compared with the 0.35 α-toco in soybean, 47.57 µg/g compared with 18.71 µg/g γ-toco or, 5.56 µg/g compared with 1.74 µg/g β-toco). The highest content of the three analyzed tocopherols was γ -tocopherol, both in callus and soybeans. Furthermore, the data showed that herbicides used in soybean culture significantly influenced both the in vitro callus production and the tocopherol callus content (p ˂ 0.05). Altogether, soybean callus can be an important source of tocopherols, and herbicides significantly influence in vitro callus production and the tocopherol callus content.
Influence of Habitat Types on Diversity and Species Composition of Urban Flora—A Case Study in Serbia
Authors: Glišić Milan , Jakovljević Ksenija , Lakušić Dmitar , Šinžar-Sekulić Jasmina , Vukojičić Snežana , Tabašević Milena , Jovanović Slobodan
Published: December 2021

The aim of this study was to investigate the floristic composition and diversity of seven urban habitat types in 24 Serbian cities with different climatic affiliation. In each of the 24 cities, we selected 1 ha plots representing a habitat from one of the following groups: square, boulevard, residential area with compact and with open building pattern, city park, and sites with early and mid-succession vegetation stages. All vascular plant species that occur spontaneously in these plots were observed. Data on the main climatic characteristics were collected for each plot, and data on the life forms were obtained for each species recorded. Diagnostic species were identified for each habitat type analyzed, and alpha, beta and gamma diversity were calculated. A total of 674 taxa were recorded in the studied area. Significant differences were observed in habitats by diagnostic species and by life form representation. The lowest alpha and gamma diversity and the dominance of therophytes were observed in habitat types with intensive anthropogenic impact, whereas the highest number was recorded in mid-successional sites and residential areas with a compact building pattern. The analysis showed that habitat type influences species composition much more than climate.
Hyperspectral Reflectance Response of Wild Rocket (Diplotaxis tenuifolia) Baby-Leaf to Bio-Based Disease Resistance Inducers Using a Linear Mixed Effect Model
Authors: Pane Catello , Galieni Angelica , Riefolo Carmela , Nicastro Nicola , Castrignanò Annamaria
Published: December 2021

Baby leaf wild rocket cropping systems feeding the high convenience salad chain are prone to a set of disease agents that require management measures compatible with the sustainability-own features of the ready-to-eat food segment. In this light, bio-based disease resistance inducers able to elicit the plant’s defense mechanism(s) against a wide-spectrum of pathogens are proposed as safe and effective remedies as alternatives to synthetic fungicides, to be, however, implemented under practical field applications. Hyperspectral-based proximal sensing was applied here to detect plant reflectance response to treatment of wild rocket beds with Trichoderma atroviride strain TA35, laminarin-based Vacciplant®, and Saccharomyces cerevisiae strain LAS117 cell wall extract-based Romeo®, compared to a local standard approach including synthetic fungicides (i.e., cyprodinil, fludioxonil, mandipropamid, and metalaxyl-m) and a not-treated control. Variability of the spectral information acquired in VIS–NIR–SWIR regions per treatment was explained by three principal components associated with foliar absorption of water, structural characteristics of the vegetation, and the ecophysiological plant status. Therefore, the following model-based statistical approach returned the interpretation of the inducers’ performances at field scale consistent with their putative biological effects. The study stated that compost and laminarin-based treatments were the highest crop impacting ones, resulting in enhanced water intake and in stress-related pigment adjustment, respectively. Whereas plants under the conventional chemical management proved to be in better vigor and health status than the untreated control.
RWLMod—Potential Model to Study Plant Tolerance in Drought Stress Conditions
Authors: Sala Florin , Herbei Mihai Valentin , Rujescu Ciprian
Published: December 2021

Rationale: Water loss by evaporation is a normal physiological process, in order to regulate plant temperature. Under conditions of thermal and water stress, water loss is accelerated compared to normal conditions, and the response of plants is variable. In extreme cases, it can lead to wilting and death of plants. It was found that the phenomenon of water loss behaved as a pattern in different plant species, given by two functions, logistics (first part of water loss) and hyperbola (second part of water loss) in relation to a moment m, at which the rate of water loss (RWL) has reached its maximum value. Method: We studied the water loss process for a series of plant samples on different plant species (Picea abies L., H. Karst; Juniperus communis L.; Pinus silvestris L.; Thuja occidentalis L.; Lamium purpureum L.; Veronica hederifolia L.), measuring the rate of weight loss (RWL) in controlled conditions. The drying of the samples was done in identical conditions (thermo-balance, 100 °C, standard temperature for drying the plant samples) with the real-time recording of the drying time simultaneously with the water loss rate (RWL) from the plant samples. The exposure time varied, depending on each species sample, and was approximately 1000 s. Results: The experimental data was recorded at intervals of every 10 s, during the entire drying period. RWL values varied from 0.024 to 0.054 g/min at the beginning of the drying process and reached maximum values after 70–100 s, having values between 0.258 g/min and 0.498 g/min. During the drying period, this indicator presented different graphic evolutions, difficult to be described with a single function. The first segment was described by a logistic function, and the second was described by a hyperbola, resulting in a model (RWLMod) which described the real phenomenon. This model and theoretical calculation were used to quantify the water loss in a time interval and, compared with empirical dates, no significant differences were observed, which indicated an increased degree of accuracy regarding the use of this model. Recommendation and novelty of work: The novelty of the work is given by the obtained model (RWLMod), which makes possible the description of RWL over the entire time interval, and ensures a good fit with the real data. It recommends the method and model in studies of plant behaviour under stress in relation to different influencing factors.
Foliar Application of Nano, Chelated, and Conventional Iron Forms Enhanced Growth, Nutritional Status, Fruiting Aspects, and Fruit Quality of Washington Navel Orange Trees (Citrus sinensis L. Osbeck)
Authors: El-Gioushy Sherif F. , Ding Zheli , Bahloul Asmaa M. E. , Gawish Mohamed S. , Abou El Ghit Hanan M. , Abdelaziz Adel M. R. A. , El-Desouky Heba S. , Sami Rokayya , Khojah Ebtihal , Hashim Taghred A. , Kheir Ahmed M. S. , Zewail Reda M. Y.
Published: December 2021

Iron (Fe) is required for most metabolic processes, including DNA synthesis, respiration, photosynthesis, and chlorophyll biosynthesis; however, Fe deficiency is common in arid regions, necessitating additional research to determine the most efficient form of absorbance. Nano-fertilizers have characteristics that are not found in their traditional equivalents. This research was implemented on Washington navel orange trees (Citrus sinensis L. Osbeck) to investigate the effect of three iron forms—nano (Fe-NPs), sulfate (FeSO4), and chelated (Fe-chelated)—as a foliar spray on the growth, fruiting aspects, and nutritional status of these trees compared to control. The highest values of the tested parameters were reported when the highest Fe-NPs level and the highest Fe-chelated (EDTA) rate were used. Results obtained here showed that the spraying of the Washington navel orange trees grown under similar environmental conditions and horticulture practices adopted in the current experiment with Fe-NPs (nanoform) and/or Fe-chelated (EDTA) at 0.1% is a beneficial application for enhancing vegetative growth, flower set, tree nutritional status, and fruit production and quality. Application of Fe-NPs and Fe-chelated (EDTA, 0.1%) increased yield by 32.0% and 25% and total soluble solids (TSS) by 18.5% and 17.0%, respectively, compared with control. Spraying Washington navel orange trees with nano and chelated iron could be considered a significant way to improve vegetative growth, fruit production, quality, and nutritional status while also being environmentally preferred in the arid regions.
Gallotannin-Enriched Fraction from Quercus infectoria Galls as an Antioxidant and Inhibitory Agent against Human Glioblastoma Multiforme
Authors: Kamarudin Nur Alisa , Nik Salleh Nik Nur Hakimah , Tan Suat Cheng
Published: December 2021

In recent years, herbal medicine has experienced rapid development in the search for alternative anticancer compounds. Various phytochemicals present in Quercus infectoria (QI) galls have been reported to trigger cytotoxic effects on many types of cancer cells. However, a specific active constituent of QI galls with the potential to inhibit highly invasive stage IV malignant brain tumor, glioblastoma multiforme (GBM), is yet to be discovered. In this study, a two-phase system composed of aqueous soxhlet extraction and methanolic enrichment fractionation was employed to extract an anticancer compound, gallotannin, from the QI galls. This optimized two-phase system successfully generated a fraction (F4) with ~71% gallotannin, verified by the TLC and HPLC assays. Astoundingly, this fraction showed significantly higher (~1.15-fold) antioxidant activities compared to its crude extract, as well as to a commercial synthetic pure gallotannin. The F4 was also found to significantly suppress GBM cell growth, better than the synthetic pure gallotannin and the QI gall crude extract, probably related to its significantly higher antioxidant property. Moreover, the inhibitory effects exerted by the F4 treatment on GBM cells were comparable to the effects of two clinically used chemo-drugs (Temozolomide and Tamoxifen), indicating its high efficiency in combating human cancer. In conclusion, this study pioneered the development of an optimized extraction procedure for enriched yield of the natural gallotannin metabolite from the galls of the QI medicinal plant with high antioxidant potential and inhibitory effects on human GBM cells.
Red Light Enhances the Antioxidant Properties and Growth of Rubus hongnoensis
Authors: Oh Hyeon Eui , Yoon Ara , Park Yoo Gyeong
Published: December 2021

The purpose of this study was to determine the effect of light quality on R.hongnoensis growth, physiology, and antioxidant properties. Five light conditions were employed, including white (control), red (R), blue (B), combined LED of R, green (G), and B at 7:1:2 (RGB), as well as combined LED of R, G, B, and far-red (Fr) at 7:1:2:1 (RGBFr). R light had the greatest growth-promoting effect based on plant height, leaf length, leaf width, stem diameter, and leaf area. However, leaf width and root length exhibited the greatest growth under RGB. The fresh and dry weight of shoots and roots were highest under R and RGB light. Photosynthesis was highest under RGB and lowest under B. Transpiration was highest in RGBFr. Stomatal conductance and photosynthetic water use efficiency were greatest under RGBFr. Total phenol content and radical scavenging activity were highest under R, while total flavonoid content was highest under RGB. Superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) activities were upregulated under W, whereas guaiacol peroxidase (GPX) activity was highest under RGB. The present results suggest that, among the tested light treatments, R light was most conductive for vegetative growth and antioxidant capacity in R. hongnoensis.
Ascorbic Acid Content and Transcriptional Profiling of Genes Involved in Its Metabolism during Development of Petals, Leaves, and Fruits of Orange (Citrus sinensis cv. Valencia Late)
Authors: Alós Enriqueta , Rey Florencia , Gil José Vicente , Rodrigo María Jesús , Zacarias Lorenzo
Published: December 2021

Citrus fruit is one of the most important contributors to the ascorbic acid (AsA) intake in humans. Here, we report a comparative analysis of AsA content and transcriptional changes of genes related to its metabolism during development of petals, leaves and fruits of Valencia Late oranges (Citrus sinensis). Petals of close flowers and at anthesis contained the highest concentration of AsA. In fruits, AsA content in the flavedo reached a maximum at color break, whereas the pulp accumulated lower levels and experienced minor fluctuations during development. AsA levels in leaves were similar to those in the flavedo at breaker stage. The transcriptional profiling of AsA biosynthetic, degradation, and recycling genes revealed a complex and specific interplay of the different pathways for each tissue. The D-galacturonic acid pathway appeared to be relevant in petals, whereas in leaves the L-galactose pathway (GGP and GME) also contributed to AsA accumulation. In the flavedo, AsA content was positively correlated with the expression of GGP of the L-galactose pathway and negatively with DHAR1 gene of the recycling pathway. In the pulp, AsA appeared to be mainly controlled by the coordination among the D-galacturonic acid pathway and the MIOX and GalDH genes. Analysis of the promoters of AsA metabolism genes revealed a number of cis-acting elements related to developmental signals, but their functionalities remain to be investigated.
Influence of Drought and Salt Stress on Durum Wheat Grain Quality and Composition: A Review
Authors: De Santis Michele Andrea , Soccio Mario , Laus Maura Nicoletta , Flagella Zina
Published: December 2021

Durum wheat is a staple crop for the Mediterranean diet because of its adaptability to environmental pressure and for its large use in cereal-based food products, such as pasta and bread, as a source of calories and proteins. Durum wheat whole grains are also highly valued for their peculiar amount of dietary fiber and minerals, as well as bioactive compounds of particular interest for their putative health-beneficial properties, including polyphenols, carotenoids, tocopherols, tocotrienols, and phytosterols. In Mediterranean environments, durum wheat is mostly grown under rainfed conditions, where the crop often experiences environmental stresses, especially water deficit and soil salinity that may induce a hyperosmotic stress. In particular, changes in C and N accumulation due to these abiotic conditions, during grain filling, can influence starch and storage protein amount and composition in durum wheat caryopsis, thus influencing yield and quality traits. Recent advancements regarding the influence of water deficit and salinity stress on durum wheat are critically discussed. In particular, a focus on stress-induced changes in (a) grain protein content and composition in relation to technological and health quality; (b) starch and dietary fiber accumulation and composition; (c) phytochemical composition; (d) health-related grain micronutrient accumulation, such as Fe and Zn.
Licorice (Glycyrrhiza glabra) Extracts-Suitable Pharmacological Interventions for COVID-19? A Review
Authors: Abraham Joji , Florentine Singarayer
Published: December 2021

Even though vaccination has started against COVID-19, people should continue maintaining personal and social caution as it takes months or years to get everyone vaccinated, and we are not sure how long the vaccine remains efficacious. In order to contribute to the mitigation of COVID-19 symptoms, the pharmaceutical industry aims to develop antiviral drugs to inhibit the SARS-CoV-2 replication and produce anti-inflammatory medications that will inhibit the acute respiratory distress syndrome (ARDS), which is the primary cause of mortality among the COVID-19 patients. In reference to these tasks, this article considers the properties of a medicinal plant named licorice (Glycyrrhiza glabra), whose phytochemicals have shown both antiviral and anti-inflammatory tendencies through previous studies. All the literature was selected through extensive search in various databases such as google scholar, Scopus, the Web of Science, and PubMed. In addition to the antiviral and anti-inflammatory properties, one of the licorice components has an autophagy-enhancing mechanism that studies have suggested to be necessary for COVID-19 treatment. Based on reviewing relevant professional and historical literature regarding the medicinal properties of licorice, it is suggested that it may be worthwhile to conduct in vitro and in vivo studies, including clinical trials with glycyrrhizic and glycyrrhetinic acids together with other flavonoids found in licorice, as there is the potentiality to provide natural interventions against COVID-19 symptoms.