bluuebunny/crossref_metadata_2025 huggingface dataset:
Pressure Sensitivity Enhancement of Porous Carbon Electrode and Its Application in Self-Powered Mechanical Sensors
Authors: Dai Keren , Wang Xiaofeng , You Zheng , Zhang He
Published: January 2019

Microsystems with limited power supplies, such as electronic skin and smart fuzes, have a strong demand for self-powered pressure and impact sensors. In recent years, new self-powered mechanical sensors based on the piezoresistive characteristics of porous electrodes have been rapidly developed, and have unique advantages compared to conventional piezoelectric sensors. In this paper, in order to optimize the mechanical sensitivity of porous electrodes, a material preparation process that can enhance the piezoresistive characteristics is proposed. A flexible porous electrode with superior piezoresistive characteristics and elasticity was prepared by modifying the microstructure of the porous electrode material and adding an elastic rubber component. Furthermore, based on the porous electrode, a self-powered pressure sensor and an impact sensor were fabricated. Through experimental results, the response signals of the sensors present a voltage peak under such mechanical effects and the sensitive signal has less clutter, making it easy to identify the features of the mechanical effects.
Laser Treatments for Improving Electrical Conductivity and Piezoresistive Behavior of Polymer–Carbon Nanofiller Composites
Authors: Caradonna Andrea , Badini Claudio , Padovano Elisa , Veca Antonino , De Meo Enea , Pietroluongo Mario
Published: January 2019

The effect of carbon nanotubes, graphene-like platelets, and another carbonaceous fillers of natural origin on the electrical conductivity of polymeric materials was studied. With the aim of keeping the filler content and the material cost as low as possible, the effect of laser surface treatments on the conductivity of polymer composites with filler load below the percolation threshold was also investigated. These treatments allowed processing in situ conductive tracks on the surface of insulating polymer-based materials. The importance of the kinds of fillers and matrices, and of the laser process parameters was studied. Carbon nanotubes were also used to obtain piezoresistive composites. The electrical response of these materials to a mechanical load was investigated in view of their exploitation for the production of pressure sensors and switches based on the piezoresistive effect. It was found that the piezoresistive behavior of composites with very low filler concentration can be improved with proper laser treatments.
Fabrication of Adhesive Resistance Surface with Low Wettability on Ti6Al4V Alloys by Electro-Brush Plating
Authors: Dong Xiaojuan , Meng Jianbing , Zhou Haian , Xu Rufeng , Bai Xue , Zhang Haiyun
Published: January 2019

Anti-adhesive Ni coatings with low wettability were successfully fabricated on Ti6Al4V substrates via an electro-brush plating method, and subsequently modified with a fluoroalkylsilane (FAS) film. The surface morphology, chemical compositions, and wettability of the as-prepared coatings were measured using scanning electron microscopy (SEM), X-ray diffractometer (XRD), Fourier transform infrared spectrophotometry (FTIR), and contact angle measurements. The results showed that the surface of Ti6Al4V substrate was endowed with flower-like structures. Each flower-like cluster was constituted by a large number of Ni ions. After surface modification of FAS, the as-prepared Ti6Al4V surface had a water contact angle as high as 151.5°, a sliding angle close to 2.1°, and a solid surface energy as low as 0.97 mJ/m2. Potentiodynamic polarization tests showed that the Ni coating could provide a stable corrosion protection. In addition, the effects of processing conditions, such as working voltage, relative velocity, electrolyte concentration, and processing time, were investigated. The mechanism of the adhesive resistance was proposed, and the low wettability of Ti6Al4V surfaces was explained by Cassie–Baxter model. As a result, it was necessary to reduce the fraction of the solid–liquid interface in order to achieve anti-adhesive surface.
Microswimmer Propulsion by Two Steadily Rotating Helical Flagella
Authors: Shum Henry
Published: January 2019

Many theoretical studies of bacterial locomotion adopt a simple model for the organism consisting of a spheroidal cell body and a single corkscrew-shaped flagellum that rotates to propel the body forward. Motivated by experimental observations of a group of magnetotactic bacterial strains, we extended the model by considering two flagella attached to the cell body and rotating about their respective axes. Using numerical simulations, we analyzed the motion of such a microswimmer in bulk fluid and close to a solid surface. We show that positioning the two flagella far apart on the cell body reduces the rate of rotation of the body and increases the swimming speed. Near surfaces, we found that swimmers with two flagella can swim in relatively straight trajectories or circular orbits in either direction. It is also possible for the swimmer to escape from surfaces, unlike a model swimmer of similar shape but with only a single flagellum. Thus, we conclude that there are important implications of swimming with two flagella or flagellar bundles rather than one. These considerations are relevant not only for understanding differences in bacterial morphology but also for designing microrobotic swimmers.
Comparing Schwertmannite and Hydrobasaluminite Dissolution in Ammonium Oxalate (pH 3.0): Implications for Metal Speciation Studies by Sequential Extraction
Authors: Sánchez-España Javier , Reyes Jesús
Published: January 2019

The “poorly crystalline iron oxy-hydroxides” are one of the most reactive and environmentally important fractions in soils and sediments due to the association of many toxic elements associated with these minerals. The metal content of this fraction in sequential extraction procedures is usually evaluated by dissolution in ammonium oxalate ([NH4]2C2O4·H2O) at pH 3.0 and 25 °C. Such chemical treatment, however, may also dissolve other mineral phases of comparable reactivity, which can lead to wrong interpretations of mineral carriers for specific metals. In this study, we compare the dissolution kinetics of schwertmannite and hydrobasaluminite, two minerals of comparable crystallinity and reactivity that play a major role in the mobility of many trace metals in waters and sediments affected by acid mine drainage (AMD). We first synthesized these two minerals in the laboratory by partial neutralization of two different metal-rich mine waters, and then we applied the standard protocol of ammonium oxalate dissolution to different specimens; the solutions were periodically sampled at intervals of 2, 5, 10, 15, 30 and 60 min to compare (i) the kinetics of mineral dissolution, and (ii) the metals released during dissolution of these two minerals. The results indicate a very similar kinetics of mineral dissolution, though hydrobasaluminite exhibited a faster rate. Some toxic elements such as As, Cr or V were clearly bonded to schwertmannite, while many others such as Cu, Zn, Si, Co, Ni and Y were clearly linked to hydrobasaluminite. These results suggest that studies linking the mobility of many elements with the Fe cycle in AMD-affected soils and sediments could be inaccurate, since these elements could actually be associated with Al minerals of poor crystallinity. The step of ammonium oxalate dissolution in sequential extraction studies should be best described with a more general term such as “low-crystallinity oxy-hydroxides”.
A Computer-Vision-Guided Robot Arm for Automatically Placing Grids in Pioloform Film Preparation
Authors: Peurla Markus , Hänninen Pekka E. , Eskelinen Eeva-Liisa
Published: January 2019

Preparing pioloform/formvar support films on transmission electron microscopy (TEM) grids is a routine laboratory procedure in practically all electron microscopy units. In current practice, these grids are manually placed on the support film one by one using special tweezers, a process requiring a steady hand. The work is often ergonomically awkward to continue for a longer period of time. In this article, we describe a low-cost, computer vision-guided robot arm that automatically places the grids on the film. The success rate of the prototype robot is 90%, which is comparable to an experienced laboratory technician.
Turn-On Fluorescence Aptasensor on Magnetic Nanobeads for Aflatoxin M1 Detection Based on an Exonuclease III-Assisted Signal Amplification Strategy
Authors: Zhang Fuyuan , Liu Linyang , Ni Shengnan , Deng Jiankang , Liu Guo-Jun , Middleton Ryan , Inglis David W. , Wang Shuo , Liu Guozhen
Published: January 2019

In order to satisfy the need for sensitive detection of Aflatoxin M1 (AFM1), we constructed a simple and signal-on fluorescence aptasensor based on an autocatalytic Exonuclease III (Exo III)-assisted signal amplification strategy. In this sensor, the DNA hybridization on magnetic nanobeads could be triggered by the target AFM1, resulting in the release of a single-stranded DNA to induce an Exo III-assisted signal amplification, in which numerous G-quadruplex structures would be produced and then associated with the fluorescent dye to generate significantly amplified fluorescence signals resulting in the increased sensitivity. Under the optimized conditions, this aptasensor was able to detect AFM1 with a practical detection limit of 9.73 ng kg−1 in milk samples. Furthermore, the prepared sensor was successfully used for detection of AFM1 in the commercially available milk samples with the recovery percentages ranging from 80.13% to 108.67%. Also, the sensor performance was evaluated by the commercial immunoassay kit with satisfactory results.
Computational Investigation of Tuning the Electron-Donating Ability in Metal-Free Organic Dyes Featuring an Azobenzene Spacer for Dye-Sensitized Solar Cells
Authors: Rashid Md Al Mamunur , Hayati Dini , Kwak Kyungwon , Hong Jongin
Published: January 2019

A series of donor–π-conjugated spacer–acceptor (D–π–A) organic dyes featuring an azobenzene spacer were designed as chromic dyes and investigated computationally. The electron-donating strength was modified by introducing electron-donating units to the donor side. In particular, the trans–cis isomerization of the azobenzene-based dyes and its effect on the optical and electronic properties were further scrutinized. In both trans and cis conformers, a gradual increase in electron-donating strength promoted the natural charge separation between donor and acceptor moieties, thereby allowing the absorption of a longer wavelength of visible light. Importantly, the conformational change of the azobenzene bridge resulted in different absorption spectra and light-harvesting properties. The azobenzene-based dyes will open up a new research path for chromic dye-sensitized solar cells.
Prevention of Rotavirus Diarrhea in Suckling Rats by a Specific Fermented Milk Concentrate with Prebiotic Mixture
Authors: Rigo-Adrover Maria del Mar , Knipping Karen , Garssen Johan , van Limpt Kees , Knol Jan , Franch Àngels , Castell Margarida , Rodríguez-lagunas Maria J. , Pérez-Cano Francisco J.
Published: January 2019

Several microbial modulatory concepts, such as certain probiotics and prebiotics, confer protection against gastrointestinal infections, among which is acute diarrhea caused by the rotavirus (RV). Other microbiota modulators, such as postbiotics, produced during fermentation, might also have the potential to counteract RV infection. In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture—short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)—with potential to impact the intestinal microbiota composition was tested. An RV infected rat model was used to evaluate the amelioration of the infectious process and the improvement of the immune response induced by the fermented milk with prebiotic mixture. The dietary intervention caused a reduction in the clinical symptoms of diarrhea, such as severity and incidence. Furthermore, a modulation of the immune response was observed, which might enhance the reduction of the associated diarrhea. In addition, the fermented milk with prebiotic mixture was able to bind the virus and reduce its clearance. In conclusion, the postbiotic components in the fermented milk in combination with the prebiotics used here showed protective properties against RV infection.
Analysis of the Lipolytic Activity of Whole-Saliva and Site-Specific Secretions from the Oral Cavity of Healthy Adults
Authors: Lai Weng Yuen Willy , Chua Jocelyn Wei Min , Gill Saloni , Brownlee Iain A.
Published: January 2019

It is currently unclear how the process of fat digestion occurs in the mouth of humans. This pilot study therefore aimed to quantify the levels of lipolytic activity at different sites of the mouth and in whole saliva. Samples of whole saliva and from 4 discrete sites in the oral cavity were collected from 42 healthy adult participants. All samples were analyzed for lipolytic activity using two different substrates (olive oil and the synthetic 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6’-methylresorufin) ester (DGGR)). Bland–Altman analyses suggested that the two assays gave divergent results, with 91% and 23% of site-specific and 40% and 26% of whole-saliva samples testing positive for lipolytic activity, respectively. Non-parametric multiple comparisons tests highlighted that median (IQR) of lipolytic activity (tested using the olive oil assay) of the samples from the parotid 20.7 (11.7–31.0) and sublingual 18.4 (10.6–47.2) sites were significantly higher than that of whole saliva 0.0 (0.0–35.7). In conclusion, lipolysis appears to occur in the oral cavity of a proportion of individuals. These findings give a preliminary indication that lipolytic agent activity in the oral cavity may be substrate-specific but do not discount that the enzyme is from sources other than oral secretions (e.g., microbes, gastric reflux).
Carotenoid Content in Breastmilk in the 3rd and 6th Month of Lactation and Its Associations with Maternal Dietary Intake and Anthropometric Characteristics
Authors: Zielinska Monika A. , Hamulka Jadwiga , Wesolowska Aleksandra
Published: January 2019

Carotenoids are diet-dependent milk components that are important for the visual and cognitive development of an infant. This study determined β-carotene, lycopene and lutein + zeaxanthin in breastmilk and its associations with dietary intake from healthy Polish mothers in the first six months of lactation. Concentrations of carotenoids in breastmilk were measured by HPLC (high-performance liquid chromatography) (first, third, sixth month of lactation) and dietary intake was assessed based on a three-day dietary record (third and sixth month of lactation). The average age of participants (n = 53) was 31.4 ± 3.8 years. The breastmilk concentrations of carotenoids were not changed over the progress of lactation. Lycopene was a carotenoid with the highest content in breastmilk (first month 112.2 (95% CI 106.1–118.3)—sixth month 110.1 (103.9–116.3) nmol/L) and maternal diet (third month 7897.3 (5465.2–10329.5) and sixth month 7255.8 (5037.5–9474.1) µg/day). There was a positive correlation between carotenoids in breastmilk and dietary intake (lycopene r = 0.374, r = 0.338; lutein + zeaxanthin r = 0.711, r = 0.726, 3rd and 6th month, respectively) and an inverse correlation with maternal BMI in the third month of lactation (β-carotene: r = −0.248, lycopene: r = −0.286, lutein + zeaxanthin: r = −0.355). Adjusted multivariate regression models confirmed an association between lutein + zeaxanthin intake and its concentration in breastmilk (third month: β = 0.730 (0.516–0.943); 6th: β = 0.644 (0.448–0.840)). Due to the positive associations between dietary intake and breastmilk concentrations, breastfeeding mothers should have a diet that is abundant in carotenoids.
Body Fat Percentage, Body Mass Index, Fat Mass Index and the Ageing Bone: Their Singular and Combined Roles Linked to Physical Activity and Diet
Authors: Tomlinson David J. , Erskine Robert M. , Morse Christopher I. , Onambélé Gladys L.
Published: January 2019

This study took a multi-analytical approach including group differences, correlations and unit-weighed directional z-score comparisons to identify the key mediators of bone health. A total of 190 participants (18–80 years) were categorized by body fat%, body mass index (BMI) and fat mass index (FMI) to examine the effect of differing obesity criteria on bone characteristics. A subset of 50 healthy-eating middle-to-older aged adults (44–80 years) was randomly selected to examine any added impact of lifestyle and inflammatory profiles. Diet was assessed using a 3-day food diary, bone mineral density (BMD) and content (BMC) by dual energy x-ray absorptiometry in the lumbar, thoracic, (upper and lower) appendicular and pelvic areas. Physical activity was assessed using the Baecke questionnaire, and endocrine profiling was assessed using multiplex luminometry. Obesity, classed via BMI, positively affected 20 out of 22 BMC- and BMD-related outcome measures, whereas FMI was associated with 14 outcome measures and adiposity only modulated nine out of 22 BMC- and BMD-related outcome measures. Whilst bivariate correlations only linked vitamin A and relative protein intake with BMD, the Z-score composite summary presented a significantly different overall dietary quality between healthy and osteopenic individuals. In addition, bivariate correlations from the subset revealed daily energy intake, sport-based physical activity and BMI positive mediators of seven out of 10 BMD sites with age and body fat% shown to be negative mediators of bone characteristics. In conclusion, whilst BMI is a good indicator of bone characteristics, high body fat% should also be the focus of osteoporosis risk with ageing. Interestingly, high BMI in conjunction with moderate to vigorous activity supplemented with an optimal diet (quality and quantity) are identified as positive modulators of bone heath.
Dietary Protein, Kidney Function and Mortality: Review of the Evidence from Epidemiological Studies
Authors: Bilancio Giancarlo , Cavallo Pierpaolo , Ciacci Carolina , Cirillo Massimo
Published: January 2019

The World Health Organization recommends a minimum requirement of 0.8 g/day protein/kg ideal weight. Low protein diets are used against kidney failure progression. Efficacy and safety of these diets are uncertain. This paper reviews epidemiological studies about associations of protein intake with kidney function decline and mortality. Three studies investigated these associations; two reported data on mortality. Protein intake averaged >60 g/day and 1.2 g/day/kg ideal weight. An association of baseline protein intake with long-term kidney function decline was absent in the general population and/or persons with normal kidney function but was significantly positive in persons with below-normal kidney function. Independent of kidney function and other confounders, a J-curve relationship was found between baseline protein intake and mortality due to ≈35% mortality excess for non-cardiovascular disease in the lowest quintile of protein intake, a quintile where protein intake averaged <0.8 g/day/kg ideal weight. Altogether, epidemiological evidence suggests that, in patients with reduced kidney function, protein intakes of ≈0.8 g/d/kg ideal weight could limit kidney function decline without adding non-renal risks. Long-term lower protein intake could increase mortality. In most patients, an intake of ≈0.8 g/day/kg would represent a substantial reduction of habitual intake considering that average intake is largely higher.
Acknowledgement to Reviewers of Particles in 2018
Authors:   Particles Editorial Office
Published: January 2019

Rigorous peer-review is the corner-stone of high-quality academic publishing [...]
Emergence and Spread of Extended Spectrum β-Lactamase Producing Enterobacteriaceae (ESBL-PE) in Pigs and Exposed Workers: A Multicentre Comparative Study between Cameroon and South Africa
Authors: Founou Luria Leslie , Founou Raspail Carrel , Ntshobeni Noyise , Govinden Usha , Bester Linda Antoinette , Chenia Hafizah Yousuf , Djoko Cyrille Finyom , Essack Sabiha Yusuf
Published: January 2019

Extended spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) represent a significant public health concern globally and are recognized by the World Health Organization as pathogens of critical priority. However, the prevalence of ESBL-PE in food animals and humans across the farm-to-plate continuum is yet to be elucidated in Sub-Saharan countries including Cameroon and South Africa. This work sought to determine the risk factors, carriage, antimicrobial resistance profiles and genetic relatedness of extended spectrum β-lactamase producing Enterobacteriaceae (ESBL-PE) amid pigs and abattoir workers in Cameroon and South Africa. ESBL-PE from pooled samples of 432 pigs and nasal and hand swabs of 82 humans were confirmed with VITEK 2 system. Genomic fingerprinting was performed by ERIC-PCR. Logistic regression (univariate and multivariate) analyses were carried out to identify risk factors for human ESBL-PE carriage using a questionnaire survey amongst abattoir workers. ESBL-PE prevalence in animal samples from Cameroon were higher than for South Africa and ESBL-PE carriage was observed in Cameroonian workers only. Nasal ESBL-PE colonization was statistically significantly associated with hand ESBL-PE (21.95% vs. 91.67%; p = 0.000; OR = 39.11; 95% CI 2.02–755.72; p = 0.015). Low level of education, lesser monthly income, previous hospitalization, recent antibiotic use, inadequate handwashing, lack of training and contact with poultry were the risk factors identified. The study highlights the threat posed by ESBL-PE in the food chain and recommends the implementation of effective strategies for antibiotic resistance containment in both countries.
Intestinal Drug Absorption Enhancement by Aloe vera Gel and Whole Leaf Extract: In Vitro Investigations into the Mechanisms of Action
Authors: Haasbroek Anja , Willers Clarissa , Glyn Matthew , du Plessis Lissinda , Hamman Josias
Published: January 2019

The co-administration of absorption enhancing agents with macromolecular drugs (e.g., protein and peptide drugs) has been identified as a means to improve the oral bioavailability of these drugs. Absorption-enhancing agents of natural origins have received a great deal of attention due to their sustainable production, in support of green chemistry. In previous studies, certain parts of the Aloe vera leaf (e.g., gel and whole leaf extract) have shown a potential to enhance drug permeation across the intestinal epithelial barrier. The mechanism of the drug-absorption-enhancement action and the capacity for absorption-enhancement of the A. vera gel and whole leaf, were investigated in this study. A clear decrease in transepithelial electrical resistance (TEER) of Caco-2 cell monolayers exposed to A. vera gel and wholeleaf extract, in various concentrations, indicated the opening of tight junctions between the epithelial cells. The transport of Fluorescein isothiocyanate (FITC)-dextran, with a molecular weight of 4 kDa (FD-4), could be enhanced across the Caco-2 cell monolayers, by the A. vera gel and whole-leaf extract, but not the FITC-dextran with larger molecular weights (i.e., 10, 20, and 40 kDa), which indicated a limited drug absorption enhancement capacity, in terms of the molecular size. Accumulation of FD-4 between the Caco-2 cells (and not within the cells), after treatment with the A. vera gel and whole-leaf extract was shown with a confocal laser scanning microscopy (CLSM) imaging, indicating that the paracellular transport of FD-4 occurred after the interaction of the A. vera gel and whole-leaf extract, with the epithelial cell monolayers. Furthermore, changes in the F-actin distribution in the cytoskeleton of the Caco-2 cell monolayers was observed by means of a fluorescence staining, which confirmed tight junction modulation as the mechanism of action for the absorption enhancement effect of the A. vera gel and whole-leaf extract.
Comparative Analysis of Protein Quantification Methods for the Rapid Determination of Protein Loading in Liposomal Formulations
Authors: Hussain Maryam T. , Forbes Neil , Perrie Yvonne
Published: January 2019

Advances in manufacturing processes provide the ability for the high throughput production of liposomes containing a range of moieties, from small molecules to large biologicals (including proteins and nucleic acids for prophylactic and therapeutic applications). Whilst rapid quantification methods for small molecules are generally well established, the ability to rapidly quantify liposomal entrapment of proteins is limited. Indeed, most standard protein quantification techniques (including the BCA assay and Reverse phase-high performance liquid chromatography (RP-HPLC)) measure protein encapsulation indirectly, by measuring the amount of non-incorporated drug, and subtracting from the initial amount of protein added. However, this can give inaccurate and misrepresentative results. To address this, we have developed a range of methods to directly quantify protein entrapment within liposomes. The encapsulation efficiency within neutral, anionic and cationic liposome formulations was determined by three techniques; BCA assay, RP-HPLC and HPLC coupled to an evaporative light scattering detector, (HPLC-ELSD). All three methods are reliable for the quantification of protein, with linear responses and correlation coefficients of 0.99, and LOQ for all three methods being less than 10 µg/mL. Here within, we provide three methods for the rapid and robust quantification of protein loading within liposomal (and other bilayer) vesicle systems.
Layer-by-Layer Assembly and Electrochemical Study of Alizarin Red S-Based Thin Films
Authors: Ma Wei , Zhang Yanpu , Li Fei , Kou Donghui , Lutkenhaus Jodie L.
Published: January 2019

Electroactive organic dyes incorporated in layer-by-layer (LbL) assemblies are of great interest for a variety of applications. In this paper, Alizarin Red S (ARS), an electroactive anthraquinone dye, is employed to construct LbL (BPEI/ARS)n films with branched poly(ethylene imine) (BPEI) as the complementary polymer. Unconventional LbL methods, including co-adsorption of ARS and poly(4-styrene sulfonate) (PSS) with BPEI to assemble (BPEI/(ARS+PSS))n, as well as pre-complexation of ARS with BPEI and further assembly with PSS to fabricate ((BPEI+ARS)/PSS)n, are designed for investigation and comparison. Film growth patterns, UV–Vis spectra and surface morphology of the three types of LbL assemblies are measured and compared to reveal the formation mechanism of the LbL films. Electrochemical properties including cyclic voltammetry and spectroelectrochemistry of (BPEI/ARS)120, (BPEI/(ARS+PSS))120 and ((BPEI+ARS)/PSS)120 films are studied, and the results show a slight color change due to the redox reaction of ARS. ((BPEI+ARS)/PSS)120 shows the best stability among the three samples. It is concluded that the manner of dye- incorporation has a great effect on the electrochemical properties of the resultant films.
Influence of the Characteristics of Expandable Graphite on the Morphology, Thermal Properties, Fire Behaviour and Compression Performance of a Rigid Polyurethane Foam
Authors: Acuña Pablo , Li Zhi , Santiago-Calvo Mercedes , Villafañe Fernando , Rodríguez-Perez Miguel Ángel , Wang De-Yi
Published: January 2019

Three types of expandable graphite (EG) differing in particle size and expansion volume, are compared as flame retardant additives to rigid polyurethane foams (RPUFs). In this paper we discuss microstructure, thermal stability, fire behavior, and compression performance. We find that ell size distributions were less homogeneous and cell size was reduced. Furthermore, thermal conductivity increased along with EG loading. Thermogravimetric analysis (TGA) showed that EG only increased residue yield differently. The results indicate that a higher expansion of EG increased the limiting oxygen index (LOI) value, whereas a bigger particle size EG improved the rating of the vertical burning test (UL94). Results from the cone calorimeter test showed that a bigger particle size EG effectively reduced peak of heat release rate (pHRR). Furthermore, a higher expansion, led to a decrease in smoke production (TSP). The combination of both characteristics gives extraordinary results. The physical–mechanical characterization of the EG/RPUF foams revealed that their compression performance decreased slightly, mostly due to the effect of a bigger size EG.
Towards Detection of Glycoproteins Using Molecularly Imprinted Nanoparticles and Boronic Acid-Modified Fluorescent Probe
Authors: Jiang Lingdong , Lu Rui , Ye Lei
Published: January 2019

Glycoproteins represent a group of important biomarkers for cancer and other life-threatening diseases. Selective detection of specific glycoproteins is an important step for early diagnosis. Traditional glycoprotein assays are mostly based on lectins, antibodies, and enzymes, biochemical reagents that are costly and require special cold chain storage and distribution. To address the shortcomings of the existing glycoprotein assays, we propose a new approach using protein-imprinted nanoparticles to replace the traditional lectins and antibodies. Protein-imprinted binding sites were created on the surface of silica nanoparticles by copolymerization of dopamine and aminophenylboronic acid. The imprinted nanoparticles were systematically characterized by dynamic light scattering, scanning and transmission electron microscopy, thermogravimetric analysis, Fourier transform infrared spectroscopy, and elemental analysis. A boronic acid-modified fluorescent probe was used to detect the target glycoprotein captured by the imprinted nanoparticles. Using horseradish peroxidase as a model glycoprotein, we demonstrated that the proposed method can be applied to detect target protein containing multiple glycosylation sites. Because of their outstanding stability and low cost, imprinted nanoparticles and synthetic probes are attractive replacements of traditional biochemical reagents to develop simpler, faster, and more cost-effective analytical methods for glycoproteins.
Biocompatibility of Small-Diameter Vascular Grafts in Different Modes of RGD Modification
Authors: Antonova Larisa V. , Silnikov Vladimir N. , Sevostyanova Victoria V. , Yuzhalin Arseniy E. , Koroleva Lyudmila S. , Velikanova Elena A. , Mironov Andrey V. , Godovikova Tatyana S. , Kutikhin Anton G. , Glushkova Tatiana V. , Serpokrylova Inna Yu. , Senokosova Evgeniya A. , Matveeva Vera G. , Khanova Mariam Yu. , Akentyeva Tatiana N. , Krivkina Evgeniya O. , Kudryavtseva Yulia A. , Barbarash Leonid S.
Published: January 2019

Modification with Arg-Gly-Asp (RGD) peptides is a promising approach to improve biocompatibility of small-calibre vascular grafts but it is unknown how different RGD sequence composition impacts graft performance. Here we manufactured 1.5 mm poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(ε-caprolactone) grafts modified by distinct linear or cyclic RGD peptides immobilized by short or long amine linker arms. Modified vascular prostheses were tested in vitro to assess their mechanical properties, hemocompatibility, thrombogenicity and endothelialisation. We also implanted these grafts into rat abdominal aortas with the following histological examination at 1 and 3 months to evaluate their primary patency, cellular composition and detect possible calcification. Our results demonstrated that all modes of RGD modification reduce ultimate tensile strength of the grafts. Modification of prostheses does not cause haemolysis upon the contact with modified grafts, yet all the RGD-treated grafts display a tendency to promote platelet aggregation in comparison with unmodified counterparts. In vivo findings identify that cyclic Arg-Gly-Asp-Phe-Lys peptide in combination with trioxa-1,13-tridecanediamine linker group substantially improve graft biocompatibility. To conclude, here we for the first time compared synthetic small-diameter vascular prostheses with different modes of RGD modification. We suggest our graft modification regimen as enhancing graft performance and thus recommend it for future use in tissue engineering.
Viability of the Use of Leachates from a Mechanical Biological Municipal Solid Waste Treatment Plant as Fertilizers
Authors: Cardoso Jonathan , Gomes Helder T. , Brito Paulo
Published: January 2019

The main environmental issue associated with compost production is the production of a liquid leachate. Leachate from municipal wastes contains carbon, nitrogen, phosphorus, potassium and trace elements that can be used as nutrients by plants. The advantages of the use of organic wastes such as compost leachate as fertilizers are evident. Their use would reduce the consumption of commercial fertilizers, which need, with their production, high cost and energy. This work aims to determine the physical and chemical properties of a specific leachate with a variable composition, collected from the composting line of a mechanical and biological treatment facility. The goal is to assess if the leachates can be used as a potential source for fertilizers, and thus develop and design a sequence of processes which could effectively convert the leachates to commercial fertilizers according to the requirements of the proposal of regulation of the European Parliament of 2016 for fertilizers. Preliminary results show that the leachate samples qualitatively meet the requirements established for the composition of commercial fertilizers, especially organo-mineral fertilizers. Furthermore, there is no production cost of leachate as a raw material. The results show that the leachate is characterized by manageable concentrations of heavy metals which can be removed by adsorption processes, and it presents suitable amounts of organic carbon after a water removal procedure. However, the establishment of the conditions for suitable conversion processes are still under investigation considering the high composition variability due to factors like storage and environmental conditions.
The Fullness of Time: Kierkegaardian Themes in Dreyer’s Ordet
Authors: Watts Daniel
Published: January 2019

I offer an approach to Dreyer’s film Ordet as a contribution to the phenomenology of a certain kind of religious experience. The experience in question is one of a moment that disrupts the chronological flow of time and that, in the lived experience of it, is charged with eternal significance. I propose that the notoriously divisive ending of Ordet reflects an aim to provide the film’s viewers with an experience of this very sort. I draw throughout on some central ideas in Kierkegaard’s work, especially his category of ‘the moment.’
Religion without God? Approaches to Theological Reference in Modern and Contemporary Jewish Thought
Authors: Fisher Cass
Published: January 2019

Recent scholarship on both ancient and modern Judaism has criticized the identification of Judaism as a religion. From the perspective of the modern period, what has remained unaddressed is the very peculiar religion that Jewish philosophers and theologians have formed. Numerous scholars with varying philosophical and religious commitments depict Judaism as a religion in which belief plays a negligible role and reference to God is tenuous if not impossible. This article charts three trends in modern and contemporary Jewish thought on the subject of theological reference: restricted referentialism, ostensive referentialism, and theological referentialism. The article concludes by discussing new developments in the theory of reference that can further the work of the theological referentialists and help revitalize Jewish theology.
Model Simulation and Prediction of Decadal Mountain Permafrost Distribution Based on Remote Sensing Data in the Qilian Mountains from the 1990s to the 2040s
Authors: Zhao Shangmin , Zhang Shifang , Cheng Weiming , Zhou Chenghu
Published: January 2019

Based on the results of remote sensing data interpretation, this paper aims to simulate and predict the mountain permafrost distribution changes affected by the mean decadal air temperature (MDAT), from the 1990s to the 2040s, in the Qilian Mountains. A bench-mark map is visually interpreted to acquire a mountain permafrost distribution from the 1990s, based on remote sensing images. Through comparison and estimation, a logistical regression model (LRM) is constructed using the bench-mark map, topographic and land coverage factors and MDAT data from the 1990s. MDAT data from the 2010s to the 2040s are predicted according to survey data from meteorological stations. Using the LRM, MDAT data and the factors, the probabilities (p) of decadal mountain permafrost distribution from the 1990s to the 2040s are simulated and predicted. According to the p value, the permafrost distribution statuses are classified as ‘permafrost probable’ (p > 0.7), ‘permafrost possible’ (0.7 ≥ p ≥ 0.3) and ‘permafrost improbable’ (p < 0.3). From the 1990s to the 2040s, the ‘permafrost probable’ type mainly degrades to that of ‘permafrost possible’, with the total area degenerating from 73.5 × 103 km2 to 66.5 × 103 km2. The ‘permafrost possible’ type mainly degrades to that of ‘permafrost impossible’, with a degradation area of 6.5 × 103 km2, which accounts for 21.3% of the total area. Meanwhile, the accuracy of the simulation results can reach about 90%, which was determined by the validation of the simulation results for the 1990s, 2000s and 2010s based on remote sensing data interpretation results. This research provides a way of understanding the mountain permafrost distribution changes affected by the rising air temperature rising over a long time, and can be used in studies of other mountains with similar topographic and climatic conditions.
Reduce Calibration Time in Motor Imagery Using Spatially Regularized Symmetric Positives-Definite Matrices Based Classification
Authors: Singh Amardeep , Lal Sunil , Guesgen Hans W.
Published: January 2019

Electroencephalogram (EEG) based motor imagery brain–computer interface (BCI) requires large number of subject specific training trials to calibrate the system for a new subject. This results in long calibration time that limits the BCI usage in practice. One major challenge in the development of a brain–computer interface is to reduce calibration time or completely eliminate it. To address this problem, existing approaches use covariance matrices of electroencephalography (EEG) trials as descriptors for decoding BCI but do not consider the geometry of the covariance matrices, which lies in the space of Symmetric Positive Definite (SPD) matrices. This inevitably limits their performance. We focus on reducing calibration time by introducing SPD based classification approach. However, SPD-based classification has limited applicability in small training sets because the dimensionality of covariance matrices is large in proportion to the number of trials. To overcome this drawback, our paper proposes a new framework that transforms SPD matrices in lower dimension through spatial filter regularized by prior information of EEG channels. The efficacy of the proposed approach was validated on the small sample scenario through Dataset IVa from BCI Competition III. The proposed approach achieved mean accuracy of 86.13 % and mean kappa of 0.72 on Dataset IVa. The proposed method outperformed other approaches in existing studies on Dataset IVa. Finally, to ensure the robustness of the proposed method, we evaluated it on Dataset IIIa from BCI Competition III and Dataset IIa from BCI Competition IV. The proposed method achieved mean accuracy 92.22 % and 81.21 % on Dataset IIIa and Dataset IIa, respectively.
SWIR AOTF Imaging Spectrometer Based on Single-pixel Imaging
Authors: Zhao Huijie , Xu Zefu , Jiang Hongzhi , Jia Guorui
Published: January 2019

An acousto-optic tunable filter (AOTF) is a new type of mono-wavelength generator, and an AOTF imaging spectrometer can obtain spectral images of interest. However, due to the limitation of AOTF aperture and acceptance angle, the light passing through the AOTF imaging spectrometer is weak, especially in the short-wave infrared (SWIR) region. In weak light conditions, the noise of a non-deep cooling mercury cadmium telluride (MCT) detector is high compared to the camera response. Thus, effective spectral images cannot be obtained. In this study, the single-pixel imaging (SPI) technique was applied to the AOTF imaging spectrometer, which can obtain spectral images due to the short-focus lens that collects light into a small area. In our experiment, we proved that the irradiance of a short-focus system is much higher than that of a long-focus system in relation to the AOTF imaging spectrometer. Then, an SPI experimental setup was built to obtain spectral images in which traditional systems cannot obtain. This work provides an efficient way to detect spectral images from 1000 to 2200 nm.
Behavioral Pedestrian Tracking Using a Camera and LiDAR Sensors on a Moving Vehicle
Authors: Dimitrievski Martin , Veelaert Peter , Philips Wilfried
Published: January 2019

In this paper, we present a novel 2D–3D pedestrian tracker designed for applications in autonomous vehicles. The system operates on a tracking by detection principle and can track multiple pedestrians in complex urban traffic situations. By using a behavioral motion model and a non-parametric distribution as state model, we are able to accurately track unpredictable pedestrian motion in the presence of heavy occlusion. Tracking is performed independently, on the image and ground plane, in global, motion compensated coordinates. We employ Camera and LiDAR data fusion to solve the association problem where the optimal solution is found by matching 2D and 3D detections to tracks using a joint log-likelihood observation model. Each 2D–3D particle filter then updates their state from associated observations and a behavioral motion model. Each particle moves independently following the pedestrian motion parameters which we learned offline from an annotated training dataset. Temporal stability of the state variables is achieved by modeling each track as a Markov Decision Process with probabilistic state transition properties. A novel track management system then handles high level actions such as track creation, deletion and interaction. Using a probabilistic track score the track manager can cull false and ambiguous detections while updating tracks with detections from actual pedestrians. Our system is implemented on a GPU and exploits the massively parallelizable nature of particle filters. Due to the Markovian nature of our track representation, the system achieves real-time performance operating with a minimal memory footprint. Exhaustive and independent evaluation of our tracker was performed by the KITTI benchmark server, where it was tested against a wide variety of unknown pedestrian tracking situations. On this realistic benchmark, we outperform all published pedestrian trackers in a multitude of tracking metrics.
Promoting Stair Climbing as an Exercise Routine among Healthy Older Adults Attending a Community-Based Physical Activity Program
Authors: Hongu Nobuko , Shimada Mieko , Miyake Rieko , Nakajima Yusuke , Nakajima Ichirou , Yoshitake Yutaka
Published: January 2019

Stair climbing provides a feasible opportunity for increasing physical activity (PA) in daily living. The purpose of this study was to examine the daily walking and stair-climbing steps among healthy older adults (age: 74.0 ± 4.9 years; Body Mass Index (BMI): 22.3 ± 2.5 kg/m2). Participants (34 females and 15 males) attended a weekly 6-month community-based PA program. During the entire program period, daily walking and stair-climbing steps were recorded using a pedometer (Omron, HJA-403C, Kyoto, Japan). Before and after the 6-month program, height, body weight and leg muscle strength were assessed. After the 6-month program, the mean walking and stair-climbing steps in both women and men increased significantly (p ≤ 0.01). Daily stair-climbing steps increased about 36 steps in women and 47 steps in men. At the end of 6 months, only male participants had significant correlation between the number of stair steps and leg muscle strength (r = 0.428, p = 0.037). This study reported that healthy older adults attending the community-based PA program had regular stair-climbing steps during daily living. Promoting stair climbing as an exercise routine was feasible to increase their walking and stair-climbing steps.
Towards Sustainable Wood-Based Energy: Evaluation and Strategies for Mainstreaming Sustainability in the Sector
Authors: Szulecka Julia
Published: January 2019

Bioenergy, mostly from wood biomass, is now widely seen as an important element in the efforts to tame dangerous climate change. At the same time, foresters and development specialists note that wood-based energy production can contribute to rural development. However, to deliver on these two goals without generating negative side effects, wood-based energy has to be sustainable, while currently, the sector is developing rapidly in ways that are technologically advanced, with questionable sustainability. How can sustainability be achieved in bioenergy production, to make it a viable element of climate change mitigation, adaptation, and rural development? Arguing for the need to mainstream sustainability thinking into wood-based energy production, the article draws on a critical literature review to identify four different levels of sustainability in the existing research on bioenergy from wood. It shows two possible strategies for integrating sustainability in wood bioenergy production. A top-down approach draws on global forestry governance instruments, while a bottom-up approach uses best-practices in forest plantations for bioenergy purposes, as illustrated by a case study from rural Paraguay. Using aggregated and visualized sustainability indicators, the article exemplifies what sustainable bioenergy production means in more tangible terms.
Climate Change and Healthcare Sustainability in the Agincourt Sub-District, Kruger to Canyons Biosphere Region, South Africa
Authors: dos Santos Monika , Howard David , Kruger Pieter , Banos Arnaud , Kornik Saul
Published: January 2019

As low-income communities are most vulnerable to climate-associated health concerns, access to healthcare will increase in importance as a key priority in South Africa. This study explores healthcare sustainability in the Agincourt sub-district, Kruger to Canyons Biosphere Region in Mpumalanga, South Africa. A rapid assessment and response methodology (RAR) was implemented, which includes the examination of previous studies conducted in the sub-district, the mapping of healthcare facilities in the area, and the implementation of a facility infrastructure and workforce capacity investigation by means of key informant (KI) interviews at eight healthcare facilities. Findings indicate that the greatest need across the facilities relate to access to medical doctors and pharmacists. None of the facilities factored climate associations with health into their clinical care strategies. The necessity to train healthcare facility staff on aspects related to climate change, health, and sustainability is highlighted. Environmental health practitioners should also be incorporated in grassroots community climate adaptation strategies. Outcomes further indicate the need for the advancement of integrated healthcare and climate adaptation strategies that focus on strengthening healthcare systems, which may include novel technological approaches such as telemedicine. Policy makers need to be proactive and pre-emptive in finding and improving processes and models to render healthcare services prepared for climate change.