bluuebunny/crossref_metadata_2025 huggingface dataset:
Avaliação das Experiências Finalistas e Vencedoras do Prêmio Fundação do Banco do Brasil à Luz da Teoria das Tecnologias Sociais
Authors: Kumasaka Júlia Mitsue Vieira Cruz , Alves Fernanda Salvador
Published: August 2022

A pesquisa sistematizou as informações das experiências finalistas e vencedoras do PFBBTS, para a seguir avaliar se elas se encaixavam nos critérios teóricos definidos pelo Instituto de Tecnologia Social (ITS). Foram analisados documentos, vídeos e website de 113 experiências, de forma quali-quantitativa. A sistematização das informações mostrou que essas experiências são descentralizadas geograficamente no país e já há experiências na América Latina. Sugere-se que as temáticas, ODS e populações-alvo menos abordados sejam estimuladas em prêmios futuros. Porém, notou-se que nem todas as características teóricas foram percebidas nas experiências analisadas, com menor presença daquelas que garantiam a emancipação dos atores e sua participação ativa. Indica-se a possibilidade da Fundação Banco do Brasil rever as características para cadastrar e principalmente conceder o prêmio as experiências. Assim, maior alinhamento entre teoria e prática poderia ser implementado. O artigo apresentou um conceito (com base em diversos autores) de Tecnologia Social e discutiu suas características teóricas, gerando uma compreensão mais detalhada do seu significado, bem como apresentando uma correlação entre elas. Entre recomendações de pesquisas futuras, tem-se: estudos quali e/ou quantitativos acerca dos benefícios (econômicos, sociais e ambientais) da sua implantação, parcerias inter e intrasetorial necessárias para sua efetividade, descrição e análise das características em TS, mapeamento de TS numa mesma região geográfica e atingimento das metas dos ODS e a interrelação deles num mesma Tecnologia Social.
Estudo Morfoanatômico e Atividade Antimicrobiana das Folhas de Miconia ferruginata DC
Authors: Ferreira Heleno Dias , Lima Lucas Augusto Rocha , Rezende Maria Helena , Paula José Realino de , Alves Virgínia Farias , Chaul Luiza Toubas , Fiuza Tatiana de Sousa
Published: August 2022

Miconia feruginata DC. (Melastomataceae) é usada popularmente para tratamento de doenças de pele. Objetivou-se realizar o estudo morfo-anatômico, triagem fitoquímica e avaliar a atividade antimicrobiana de M. feruginata. As folhas foram coletadas em Pirenópolis, Goiás, mensalmente por 12 meses. Uma exsicata foi depositada no Herbário da UFG. A morfo-anatomia e a triagem fitoquímica foram realizadas de acordo com as técnicas convencionais e a atividade antimicrobiana pelo método de microdiluição em caldo. M. ferruginata é uma pequena árvore, com tronco suberoso, folhas simples e oposta; inflorescência em monocásio terminal, flores diclamídeas e com frutos maduros carnosos e arroxeados. Anatomicamente as folhas são hipoestomática, epiderme revestida por cutícula espessa com flanges cuticulares e criptas na face abaxial. Observou-e a presença de heterosídeos flavonoides, saponinas, cumarinas, triterpenos e taninos no pó das folhas. O extrato das folhas apresentou atividade contra Micrococcus luteus (125 µg/mL), M. luteus (250 µg/mL) e Bacillus cereus (250 µg/mL).
A Importância das Feiras para o Fortalecimento da Agricultura Urbana no Município do Rio de Janeiro
Authors: Barbosa Juliana Silva , Antunes Luiz Fernando de Sousa , Ricci Marta dos Santos Freire , Uzeda Mariella Camardelli
Published: August 2022

Com o crescente processo de urbanização, a prática de Agricultura Urbana (AU) vem ganhando destaque no cenário nacional, principalmente em grandes cidades como o Rio de Janeiro, devido à sua capacidade de produzir alimentos, melhorar a qualidade de vida e promover a soberania e segurança alimentar e nutricional (SAN) das populações urbanas. Outro fator importante é a geração de trabalho e renda para as famílias por meio da comercialização do excedente da produção, sendo a venda direta em feiras o canal mais utilizado na AU. A possibilidade de comercializar os alimentos, principalmente em feiras orgânicas/agroecológicas, valoriza e estimula o trabalho dos agricultores urbanos, invisibilizados pelo poder público. O objetivo da pesquisa foi compreender a importância das feiras para a comercialização e o fortalecimento da AU no município do Rio de Janeiro. Para isto, foram escolhidas as técnicas de entrevista e observação participativa na coleta de dados. Ao analisar as entrevistas, foi constatado que a maior parte dos agricultores tem a venda dos alimentos nas feiras como a principal fonte de renda. Segundo a pesquisa, 60% dos entrevistados têm outra fonte de renda e o agricultor que não possui outra fonte de renda, comercializa os alimentos em mais de um local. Dessa forma, pode-se inferir que aqueles agricultores que comercializam em mais de um local, conseguem, através da AU, gerar renda para o sustento da família.
Justiça Ambiental e Ecologia Política: Entre sinergias e Benefícios Mútuos na Direção de Futuros Sociais Desejáveis
Authors: Sardão Maíra , Silva Pedro Gabriel
Published: August 2022

Este artigo reúne os principais debates promovidos pela Justiça Ambiental (JA) e sua aproximação à Ecologia Política (EP). Para tal, observaram-se as perspectivas teóricas que contribuíram para a evolução do conceito de JA, a forma como tem sido definido seu campo de investigação empírico, com destaque para os principais métodos e técnicas utilizados, assim como as sinergias resultantes do seu entrelaçamento com o campo da EP. Busca-se, com isso, entender a complexidade das atuais questões socioambientais e, ao final, identificar futuros caminhos de investigação de realidades assentes na justiça social e ecológica. Neste sentido, optou-se pela revisão sistemática de literatura somada à revisão bola-de-neve. O tratamento bibliométrico permitiu agrupar os artigos pesquisados em quatro principais eixos de investigação, assim segmentados: 1. condições materiais com perspectivas à mudança; 2. relações de poder e resistências ao racional econômico; 3. politização das narrativas da sustentabilidade; 4. ativismo intercultural e emancipação dos atores. Mesmo diante da amplitude e diversificação das frentes teórico-empíricas utilizadas pelos autores dos artigos consultados, a ideia normativa de justiça unida à PE mostrou-se central nos múltiplos caminhos propostos na direção de futuros sociais desejáveis.
Aquicultura como Ferramenta na Educação: Uma Revisão Sistemática
Authors: Vilaça Vinícius Lima Tavares , Dias Thaís Vilas Boas , Navarro Rodrigo Diana
Published: August 2022

A aquicultura vem apresentando um expressivo crescimento tanto no setor alimentício quanto na educação. Tal sistema de produção pode ser utilizado de diversas formas e para várias finalidades. O presente estudo é uma revisão sistemática que aborda a questão de como a aquicultura vem sendo utilizada na educação, bem como seus focos, metodologias, soluções, público alvo e região. Observou-se que a tecnologia esteve muito presente nos trabalhos obtidos, assim como a possibilidade de diversos níveis e estilos educacionais. Também se observou que a extensão rural, a análise de cursos e currículo, vivências, experimentos e projetos de metodologia ativa estiveram presentes nos resultados. As regiões se concentraram nas Américas como um todo, na Europa e Ásia, porém não foram encontrados, através das filtragens estabelecidas, estudos na África ou Oceania. Através dos resultados, constatou-se a importância e possibilidades da aquicultura para educadores e alunos, além de demonstrar a necessidade de mais interações entre essas áreas e a tecnologia.
Efeitos da Urbanização para a Degradação das Áreas de Preservação Permanente: O Caso do Córrego Arareau no Município de Rondonópolis, Mato Grosso
Authors: Silva Samilli Santilli Braga da , Vasconcelos Monalisa Janaya Castelo da Silva , Aguiar Leonardo Orlando Campos de , Macedo Luís Otávio Bau
Published: August 2022

O artigo apresenta um estudo bibliográfico e análise por imagens georreferenciadas sobre os efeitos da urbanização e os impactos gerados para a degradação do corrégo Arareau, no perímetro urbano do município de Rondonópolis, Mato Grosso. O objetivo desta pesquisa foi elucidar as alterações e os possíveis impactos gerados ao córrego Arareau, por conta da falta de planejamento e crescimento desordenado da urbanização no município de Rondonópolis - MT. Os resultados apontam que os efeitos são severos e que existe grande supressão vegetal, ocupação irregular da mata ciliar, erosão e lançamento de carga de esgoto via galeria pluvial ao longo de todo córrego. O estudo aponta a realidade da Áreas de Preservação Permanente (APP) do córrego Arareau no decorrer do processo da expansão urbana, evidencia que o  aumento gradativo e irregular do crescimento urbano ao longo do perímetro da cidade trouxe consequências ambientais para o curso fluvial  e redução da capacidade de suporte para o abastecimento municipal. Constatou-se que a Área de Preservação Permanente (APP) total do córrego Arareau, nos limites do perímetro urbano, do município de Rondonópolis, corresponde a 126 hectares, tendo em vista uma área de proteção de 50 metros, para os cursos d’água que tenham de 10 a 50 metros de largura. Aferiu-se ainda que, desse total, cerca de 31,31 hectares encontram-se degradados (25%). Os resultados do estudo apontam para a necesssidade de uma intervenção urgente e imediata, que possibilite a redução dos impactos ambientais atuais, visando a preservação do ecossistema da área do córrego, responsável pela proteção das áreas de preservação permanente, bem como do corpo hídrico que atualmente encontra-se em condições de reversão. Nesse sentido serão necessários esforços do poder público municipal e mobilização da iniciativa privada, alcançando toda a sociedade civil.
Spatial Structure of National and International Scientific Collaboration in the Brazilian Cerrado Research
Authors: Parreira Micael Rosa , Soares Philip Teles , Nabout João Carlos
Published: August 2022

The number of authors in papers has increased over the years, indicating collaborative trends in Science and Technology. Besides, scientific collaboration is structured at different spatial scales, for example, within or between institutions in the same country or among countries. Here, we evaluate the scientific collaboration patterns at national and international levels in the Cerrado research. We searched all papers about the Cerrado published between 1945 and 2017 in the Web of Science database. We performed network analyses using pairwise distance matrices to create national and international collaboration networks. We also used spatial correlograms to test the effect of geographic distance on scientific collaboration. The number of papers increased over the years (rs = 0.96), where papers with 3-5 authors had the highest growth rate (rs = 0.96). Moreover, authors from geographically closer institutions tend to collaborate more at the national level, while we found no geographic effect on international collaboration. These results show that Brazilian scientists studying the Cerrado have collaborated more over the years regardless of distance, although locally, scientists are still more likely to work with scientists of close institutions within the biome. This collaboration tendency may be associated with the need in science to deal with more complex and multidisciplinary issues, where collaborative studies promote a greater scientific and social impact.
Are Regulatory Standards Enough to Leverage Forest Restoration? A Brazilian Case Study
Authors: Moura Ciro Jose Ribeiro de , Nunes Maria Fernanda S. Quintela da C.
Published: August 2022

Environmental offsetting is a compensation for a deforested or degraded area usually in the form of forest recovery. We provide an update on the ongoing regulatory standards (RS) regarding ecological restoration in Brazil, analyzing the existence of monitoring protocols. The introduction of Environmental Rural Registry (CAR) created by law in 2012 has become the main driver of local RS. The aim of CAR is to guarantee forest cover on rural properties by the force of RS, but it lacks to consider the vegetation structure, functionality, and quality of the vegetation. Currently only four states in Brazil uses a protocol that includes ecological criteria as a measurement of success. The existence of a specific legislation for forest restoration may enhance restoration effectiveness by clarifying the restoration process and regulations to those stakeholders involved in implementing restoration projects.  It is necessary for RS to include diverse technical approaches, providing the opportunity for solutions contemplating local possibilities and conditions.
Análisis de las actividades que proponen dos libros de texto de educación primaria. Un acercamiento comparativo desde la perspectiva de la inferencia informal
Authors:   Universidad Alberto Hurtado, Sánchez Acevedo Nicolás , Ruiz Hernández Blanca ,   Tecnológico de Monterrey
Published: August 2022

La investigación en educación estadística sugiere que algunas de las ideas centrales de la estadística se enseñen desde temprana edad con propuestas como la Inferencia Informal, la cual ha mostrado aportes significativos al aprendizaje de conceptos estadísticos básicos. En esta investigación se realiza un análisis de libros de texto en un nivel educativo clave de la educación primaria en Chile para el desarrollo de pensamiento inferencial: el octavo grado (13 a 14 años). Se analizan las actividades planteadas por dos textos influyentes a nivel nacional a la luz de las componentes de inferencia informal y contexto. Se muestran oportunidades de mejora de estos libros texto centrados en el estudio de la muestra y cómo actividades potencialmente ricas se desaprovechan en el desarrollo del pensamiento inferencial de manera intuitiva e informal. Si bien, algunas actividades muestran una orientación al desarrollo de este tipo de pensamiento, la mayoría de las actividades se centran en el aprendizaje de conceptos descontextualizados.
2,5-Diketopiperazines: A Review of Source, Synthesis, Bioactivity,
Structure, and MS Fragmentation
Authors: Jia Jia , Yao Jianling , Kong Jiao , Yu Axiang , Wei Jing , Dong Ying , Song Ruolan , Shan Dongjie , Zhong Xiangjian , Lv Fang , Fan Qiqi , She Gaimei
Published: August 2022

Background:

2,5-Diketopiperazines (DKPs), also called cyclic dipeptides, are
the simplest peptide derivatives in nature that are formed by the condensation of two amino
acids. They are an important category of bioactive substances with various structures.

Objective:

This review focuses on the natural sources, synthetic processes, biological
properties and MS fragmentation regularity of simple DKPs, in order to provide a reference
for exploring future scientific and therapeutic potentials of these compounds.

Method:

Pertinent information was collected and organized from several electronic scientific
databases (e.g., Web of Science, China Knowledge Resource Integrated, ScienceDirect,
PubMed, Wanfang Data and Google Scholar), PhD and MS dissertations.
There are 107 articles published from the early 20th century to 2021 that were reviewed
in this work.

Results:

DKPs have been obtained from a broad range of natural resources, including fungi,
bacteria, plants, and animals, and have been synthesized by chemical and biological
methods. DKPs have various pharmacological activities, including anticancer, antibacterial,
antithrombotic, neuron protective, analgesic, and other activities. Mass spectrometry
is the most common method for the structural analysis of DKPs. DKPs can be quickly
screened and identified by MS according to the mass spectrum fragmentation pattern.

Conclusion:

As a category of relatively unexplored compounds, DKPs have been demonstrated
to have various bioactivities, especially with antitumor and antibacterial activities.
However, the existing research on DKPs is still in the early stage, and their application in
drug development needs to be further studied.
Next-generation Sequencing for Surveillance of Antimicrobial Resistance
and Pathogenicity in Municipal Wastewater Treatment Plants
Authors: Cuetero-Martínez Yovany , de los Cobos-Vasconcelos Daniel , Aguirre-Garrido José Felix , Lopez-Vidal Yolanda , Noyola Adalberto
Published: August 2022

The World Health Organization (WHO) ranks antimicrobial resistance (AMR)
and various pathogens among the top 10 health threats. It is estimated that by 2050, the
number of human deaths due to AMR will reach 10 million annually. On the other hand,
several infectious outbreaks such as SARS, H1N1 influenza, Ebola, Zika fever, and
COVID-19 have severely affected human populations worldwide in the last 20 years. These
recent global diseases have generated the need to monitor outbreaks of pathogens and
AMR to establish effective public health strategies. This review presents AMR and pathogenicity
associated with wastewater treatment plants (WWTP), focusing on Next Generation
Sequencing (NGS) monitoring as a complementary system to clinical surveillance.
In this regard, WWTP may be monitored at three main points. First, at the inlet (raw
wastewater or influent) to identify a broad spectrum of AMR and pathogens contained in
the excretions of residents served by sewer networks, with a specific spatio-temporal location.
Second, at the effluent, to ensure the elimination of AMR and pathogens in the
treated water, considering the rising demand for safe wastewater reuse. Third, in sewage
sludge or biosolids, their beneficial use or final disposal can represent a significant risk
to public health. This review is divided into two sections to address the importance and
implications of AMR and pathogen surveillance in wastewater and WWTP, based on
NGS. The first section presents the fundamentals of surveillance techniques applied in
WWTP (metataxonomics, metagenomics, functional metagenomics, metaviromics, and
metatranscriptomics). Their scope and limitations are analyzed to show how microbiological
and qPCR techniques complement NGS surveillance, overcoming its limitations.
The second section discusses the contribution of 36 NGS research papers on WWTP
surveillance, highlighting the current situation and perspectives. In both sections, research
challenges and opportunities are presented.
Construction of Circadian Clock Signature for Tumor Microenvironment in Predicting
Survival for Cutaneous Melanoma
Authors: Zeng Ni , Xia Qingyue , Li Yueyue , Ma Liwen , Cheng Yuxin , Chen Yihe , Lu Qian , Lu Zhiyu , Wu Di , Luo Dan
Published: August 2022

Objective:

We explored circadian clock-related genes (CCRG) to establish a risk model and identify
associations with the tumor immune microenvironment in cutaneous melanoma (CM).

Methods:

Circadian clock genes were downloaded from Circadian Gene Database. To explore CM-related
circadian clock genes, we combined multivariate cox regression associated with least absolute shrinkage and
selection operator (LASSO) regression in the Cancer Genome Atlas (TCGA) and validated it in the GSE65904
dataset. Time-dependent receiver operating characteristic curve (ROC) and Kaplan-Meier analysis were calculated
to determine a CCRG risk score model. In addition, the overall survival nomograms of clinicopathological
factors and circadian clock-related gene signatures. Additionally, we evaluated the connection between
circadian clock-related genes with immune checkpoint inhibitors and immune cell infiltration.

Results:

Two circadian clock-related signatures were established. The risk model included SEMA4D (p<0.001,
HR: 0.709, 95% CI: 0.581 to 0.867) and SOD-2 (p=0.009, HR: 0.790, 95% CI: 0.663 to 0.944) in patients with
TCGA melanoma. The risk model was based on two CCRGs enriched in base excision repair, glycosylphosphatidyl
(GPI), and one carbon of the folate pathway. The overall survival was lower in the high-risk group. In addition,
the circadian-clock signature may be able to evaluate the immunotherapy response.

Conclusions:

We developed and validated a circadian signature to characterize the clinical significance and
tumor microenvironment of cutaneous melanoma, revealing that circadian rhythms may impact cutaneous melanoma.
Towards Further Understanding the Role of Curcumin in Wound Healing:
A Systems Biology Approach
Authors: Anukriti  , Dhingra Naveen , Bhardaj Ravindra , Bhardwaj Uma , Dhasmana Anupam
Published: August 2022

Background:

Curcumin, a polyphenolic compound present in the turmeric plant (Curcuma
longa) is well known for its anti-aging, anti-tumor, anti-inflammatory, anti-mutagenic and antioxidative
properties due to which turmeric has been used as a medicinal plant from ages.

Objective:

Our current study aims at finding the most potent targets of curcumin displaying efficient binding
by using various systems biology tools. Around 560 genes related to wound healing are extracted
from PubMed using the combination of words like wound healing, curcumin, Homo sapiens, etc.

Methods:

For the investigation of the mechanism of curcumin interference at the system level, proteinprotein
interaction network (PPIN) of the proteins involved in the wound healing process was generated
using the STRING database. The noise of the data generated in PPIN was removed by modulation of the
network with the help of Molecular Complex Detection (MCODE) and finding the seed proteins. GO
enrichment analysis along with network topology analysis and molecular docking will help in pinpointing
the most important and efficient curcumin binding proteins.

Results:

The findings of this study shows that besides SCR, PPARG and MAPK3, AKT3 is one of the
novel targets for wound healing as the binding affinity of AKT3 is -4.53 Kcal/mol, which is close to SRC
with the highest binding affinity with binding energy of -6.6 Kcal/mol. The binding energy of PPARG
was -6.2 Kcal/mol and for MAPK3 the binding energy was -5.95 Kcal/mol. Besides AKT3, FLT4 and
RPS6KB1 were also the novel targets of curcumin with binding affinities of -4.13 Kcal/mol and -4.04
Kcal/mol. In network analysis, we obtained PIK3R1 as a connector node which acted as a hub node with
highest betweenness score.

Conclusion:

From the results obtained, we can say that curcumin finds its role in all four stages of wound
healing and it also prevents the healing cells from turning into tumors.
Diagnostic Accuracy of F18-fluorodeoxyglucose Positron Emission Tomography-
computed Tomography for the Detection of Non-small Cell
Lung Cancer Recurrence: A Systematic Review and Meta-analysis
Authors: Chen Yunbing , Zhang Deying , Fan Ka
Published: August 2022

Background:

The non-small cell variant of lung cancer (NSCLC) has a poorer prognosis. It is typically diagnosed through non-invasive imaging. Of particular note has been FDG-PET/CT, which has been investigated across various settings with differing results.

Objective:

This study to pool the available information on the diagnostic performance of 18-F FDG PET/CT for detecting NSCLC recurrence.

Methods:

A systematic literature search was conducted across electronic databases for studies published prior to May 2021. The QUADAS tool was applied to assess study quality, and ta meta-analysis was performed to retrieve pooled estimates. Chi squared tests and I2 statistics were used to assess heterogeneity. Egger’s test and funnel plots were used to assess publication bias.

Results:

Literature search yielded 20 studies featuring data on 1,973 patients. The majority of the studies had low bias risk. The pooled sensitivity and specificity were 96% (95% CI: 91%-98%) and 93% (95% CI: 89%-95%), respectively. The LRP and LRN estimates were in the left upper quadrant of the LR scattergram, indicating that F18-FDG PET/CT can be utilized for both confirmation and exclusion.
The AUC was 0.98 (95% CI: 0.92-0.99). Fagan’s nomogram showed that F18-FDG PET/CT had good clinical utility for recurrent NSCLC diagnosis. There was considerable between-study variability (p=0.02). Funnel plot was asymmetrical, indicating the possibility of publication bias.

Conclusion:

This meta-analysis found FDG-PET/CT to be highly accurate for identifying NSCLC recurrence. However, more studies assessing this modality across different patient situations is required to strengthen the argument for changing international guidelines and practices.
Efficacy of Sinopharm Vaccine Among Stem Cell Transplant Recipients
During Two Peaks of Delta and Omicron variants of COVID-19
Authors: Ghasemi Ali , Khodashahi Rozita , Aliakbarian Mohsen , Khaleghi Ebrahim , Razmkhah Baran , Vahdatinia Mehrsa , Far Mahdi Salimi , Bakhti Omolbanin
Published: August 2022

Background:

Considering the dearth of research on the complications of Sinopharm
coronavirus disease 2019 (COVID-19) vaccine in immunocompromised individuals and the lack of
available data on COVID-19 vaccination from Iran.

Objective:

This study aimed to investigate the efficacy of vaccine of Sinopharm COVID-19 vaccine
and its complications in bone marrow transplant (BMT) recipients.

Methods:

This was a retrospective cross-sectional study conducted on 250 patients with BMT who
were referred to Montaserieh Hospital, Mashhad, Iran. Among them, 53 cases who received at least
two doses of Sinopharm COVID-19 vaccine from March to January 2021 were entered in this
study. The data were extracted from a student dissertation (Code:4000370).

Results:

Sinopharm vaccine side effects were reported only in 7.7% of the patients, and shingles
was the only serious side effect of the Sinopharm vaccine, which was observed only in one case.
The results also revealed that Sinopharm COVID-19 vaccine side effects were not related to age or
gender. Infection with the Delta variant of COVID-19 was reported in 7.5% (n=4) and no mortality
was reported among them. Vaccine failure was reported in 39.6% of the cases; however, no mortality
was reported among patients infected with the Omicron variant of COVID-19.

Conclusion:

In summary, it seems that Sinopharm COVID-19 vaccine adverse effects were not serious
among stem cell transplant recipients. However, it may lead to some severe complications in
the population. Vaccine failure against the Delta and Omicron variants of COVID-19 has been reported
among more than one-third of BMT patients; however, no mortality was observed among
BMT patients infected with the new variants of COVID-19.
Identification of Key Prognosis-related microRNAs in Early- and Late-
Stage Gynecological Cancers Based on TCGA Data
Authors: Mekala Venugopala Reddy , Hui-Shan Chiang , Jan-Gowth Chang , Ng Ka-Lok
Published: August 2022

Background:

Gynecological cancers (GCs), mainly diagnosed in the late stages of the disease, remain the leading cause of global mortality in women. microRNAs (miRNAs) have been explored as diagnostic and prognostic biomarkers of cancer. Evaluating miRNA signatures to develop
prognostic models could be useful in predicting high-risk patients with GC. Specifically, the identification of miRNAs associated with different stages of cancer can be beneficial in patients diagnosed with
cancer.

Objective:

This study aimed to identify potential miRNA signatures for constructing optimal prognostic
models in three major GCs using The Cancer Genome Atlas (TCGA) database

Methods:

Stage-specific Differentially Expressed microRNAs (DEmiRs) were identified and validated
in public and in-house expression datasets. Moreover, various bioinformatics investigations were used
to identify potential DEmiRs associated with the disease. All DEmiRs were analyzed using three penalized Cox regression models: lasso, adaptive lasso, and elastic net algorithms. The combined outcomes
were evaluated using Best Subset Regression (BSR). Prognostic DEmiR models were evaluated using
Kaplan–Meier plots to predict risk scores in patients. The biological pathways of the potential DEmiRs
were identified using functional enrichment analysis.

Results:

A total of 65 DEmiRs were identified in the three cancer types; among them, 17 demonstrated
dysregulated expression in public datasets of cervical cancer, and the expression profiles of 9 DEmiRs
were changed in CCLE-OV cells, whereas those of 10 are dysregulated in CCLE-UCEC cells. Additionally, ten miRNA expression profiles were observed to be the same as DEmiRs in three OV cancer
cell lines. Approximately 30 DEmiRs were experimentally validated in particular cancers. Furthermore,
23 DEmiRs were correlated with the overall survival of the patients. The combined analysis of the three
penalized Cox models and BSR analysis predicted eight potential DEmiRs. A total of five models based
on five DEmiRs (hsa-mir-526b, hsa-mir-508, and hsa-mir-204 in CESC and hsa-mir-137 and hsa-mir1251 in UESC samples) successfully differentiated high-risk and low-risk patients. Functional enrichment analysis revealed that these DEmiRs play crucial roles in GCs.

Conclusion:

We report potential DEmiR-based prognostic models to predict the high-risk patients with
GC and demonstrate the roles of miRNA signatures in the early- and late-stage of GCs.
Cognition, Symptomatology, and Community Living Skills in Schizophrenia
Authors: Chien Kuo Michael Chih , Sin Wai Yee , Wong Wai Nga , Michelle Lo Yi To , Elizabeth Tse Uen Ting , Helen Wong Hoi Wai , Elizabeth Tung Ka Yin , Fung Amy Dun-mi
Published: August 2022

Background:

Independence in community living is an important aspect of rehabilitation in schizophrenia. Previous studies investigating relationships of coginitive, symptomatic, and demographic factors in community living ability of schizophrenia showed inconsistent findings. These findings may be due to variations in functional measures used or a heterogenous sample of participants recruited.

Aim:

To investigate the relationships of cognitive, symptomatic and demographic factors with community living skills for people with schizophrenia living in supported living facilities.

Method:

This is a cross-sectional study. Thirty-seven adults with schizophrenia and 32 matched healthy controls were recruited. Both groups completed the Verbal Fluency Test, subtests of Cognistat, and the Color Trail Test. Additional assessments (i.e., Chinese version of St. Louis Inventory of Community Living Skills – SLICLS-C and Positive and Negative Syndrome Scale – PANSS) were used to evaluate participants with schizophrenia. Cognitive profile was compared between the two groups. Correlation analysis was used to explore the relationships of cognitive abilities, symptomatology, and demographic factors with community living skills in schizophrenia.

Results:

Results showed that participants with schizophrenia performed worse than the control group in verbal fluency, visual memory, immediate memory, delayed memory, and executive function tests. Significant moderate correlations between SLICLS-C score and participants’ years of education, positive symptoms, general psychopathology, and PANSS total score were identified.

Discussions and conclusion:

Results indicated cognitive impairments are persistent in schizophrenia who are in remission and have been partially integrated back into the community. Cognitive deficits that people with schizophrenia experience might be stable over most of the course of the illness. Participants’ years of education, positive symptoms, general psychopathology, and PANSS total score might be important moderating variables to include in future investigations related to predicting community living performance in schizophrenia.
Mitochondrial DNA Role in Zika Virus Infection
Authors: Carvalho Fabiana Rabe , Familiar-Macedo Débora , Alice Silva Andrea
Published: August 2022

Zika virus (ZIKV) is a member of the Flavivirus family. ZIKV infection
ranges from asymptomatic to a mild disease in adults. However, in 2015, ZIKV
infection became a public health emergency in the Americas associated with
neurological alterations such as Guillain-Barré syndrome (GBS) in adults and
congenital zika syndrome (CZS). By blocking type I IFN interferon signaling
pathways, ZIKV evades the immune system and infects cells expressing the T cell
immunoglobulin mucin domain-1 (TIM-1) and TAM (Tyro3, AXL, and Mer)
receptors, such as neural progenitor cells. Moreover, ZIKV seems to orchestrate a
process of astrocytic hypoxia that leads to the production of reactive oxygen species
(ROS), mitochondrial DNA (mtDNA) fragmentation, and apoptosis. In recent decades,
the active participation of mitochondria in the immuno-inflammatory response has
been reported in several pathologies. In this context, mtDNA seems to have an essential
role in triggering the innate immune response by activating inflammasomes, activating
the cyclic GMP–AMP synthase (cGAS)–stimulator of interferon genes (STING)
pathway, and also activating toll-like receptors that lead to IFN production and viral
clearance. Here, we present an overview of some mechanisms of inflammatory
response present in ZIKV infection, which contributes to mitochondrial dysfunction,
mtDNA release, and tissue damage.
Factores que determinan las propiedades fisicoquímicas de la miel de abejas: Revisión Sistemática de Literatura
Authors: Campo Barrera Oscar Iván , Hincapié Llanos Gustavo Adolfo
Published: August 2022

La miel de abeja es una compleja mezcla de carbohidratos y de otros compuestos naturales elaborados por diferentes especies del género de abejas Apis, entre ellas, Apis mellífera. La importancia de este suplemento dietético potencial se debe a los beneficios terapéuticos, profilácticos, cosméticos y nutricionales como resultado de sus propiedades fisicoquímicas. Por consiguiente, el objetivo que se planteó en esta propuesta investigativa fue el análisis de los factores que determinan las propiedades fisicoquímicas de la miel de abejas. Para ello, se realizó una revisión sistemática de literatura (rsl) utilizando bases de datos, tales como: Scopus, SciELO y Redalyc. La categoría de análisis que se definió fue las publicaciones científicas relacionadas con los factores que determinan las propiedades fisicoquímicas de la miel de abejas. La información obtenida se organizó en una matriz en Excel, y el procesamiento se hizo en el software de minería de texto Vantage Point; para correlacionar las variables y condensar el análisis de los resultados mediante tablas. Los factores determinantes más importantes encontrados fueron: fuente floral, origen botánico, origen geográfico, néctar, clima, tiempo de maduración, condiciones biofísicas de la región, especie de abeja, procesamiento y condiciones de almacenamiento. En conclusión, estos factores influyen determinantemente sobre los valores promedios reportados de algunas propiedades fisicoquímicas como: acidez libre, actividad de diastasa, azúcares reductores, cenizas, color instrumental Pfund, conductividad eléctrica (ce), hidroximetilfurfural (hmf), humedad, pH, sacarosa, gravedad específica, sólidos solubles totales (sst), proteínas, fructosa/glucosa, fructosa, maltosa, viscosidad, acidez titulable, actividad del agua (aw), sólidos totales y actividad antioxidante, entre otras.
Bilateral Corneal Perforation in a Patient with Chronic Ocular Graft-Versus-Host Disease: A Case Report and Literature Review
Authors:   Department of Translational Medicine, University of Ferrara, Ferrara, Italy, Adamo Ginevra G , D’Angelo Sergio ,   Department of Translational Medicine, University of Ferrara, Ferrara, Italy, Yu Angeli Christy ,   Department of Translational Medicine, University of Ferrara, Ferrara, Italy, Pellegrini Marco ,   Department of Translational Medicine, University of Ferrara, Ferrara, Italy, Bernabei Federico ,   Department of Ophthalmology, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, Paris, France, Scorcia Vincenzo ,   Department of Ophthalmology, University Magna Græcia of Catanzaro, Catanzaro, Italy, Giannaccare Giuseppe ,   Department of Ophthalmology, University Magna Græcia of Catanzaro, Catanzaro, Italy
Published: August 2022

Graft-versus-host disease (GVHD) is a serious complication that may occur in patients receiving allogeneic hematopoietic stem cell transplant (HSCT). GVHD occurs because of the immunological reaction between the donor’s T cells and the recipient’s antigens; GVHD may develop in different tissues, including the eye. Corneal perforation is an uncommon but vision-threatening manifestation of GVHD. We reported the case of a 65-year-old male patient who developed corneal perforation sequentially in both eyes 3 years after receiving HSCT. Conservative treatment with topical steroids and lubricants, bandage contact lens, and lacrimal punctal occlusion surgery resulted in the successful resolution of the corneal perforation with satisfactory visual recovery in the right eye. Therefore, corneal perforation can occur as the presenting manifestation of ocular GVHD. Regular ophthalmological examinations are recommended after HSCT to enable the early diagnosis of ocular GVHD and prompt treatment initiation.
The Development and Use of Chatbots in Public Health: Scoping Review
Authors: Wilson Lee , Marasoiu Mariana
Published: August 2022

Background

Chatbots are computer programs that present a conversation-like interface through which people can access information and services. The COVID-19 pandemic has driven a substantial increase in the use of chatbots to support and complement traditional health care systems. However, despite the uptake in their use, evidence to support the development and deployment of chatbots in public health remains limited. Recent reviews have focused on the use of chatbots during the COVID-19 pandemic and the use of conversational agents in health care more generally. This paper complements this research and addresses a gap in the literature by assessing the breadth and scope of research evidence for the use of chatbots across the domain of public health.

Objective

This scoping review had 3 main objectives: (1) to identify the application domains in public health in which there is the most evidence for the development and use of chatbots; (2) to identify the types of chatbots that are being deployed in these domains; and (3) to ascertain the methods and methodologies by which chatbots are being evaluated in public health applications. This paper explored the implications for future research on the development and deployment of chatbots in public health in light of the analysis of the evidence for their use.

Methods

Following the PRISMA-ScR (Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews) guidelines for scoping reviews, relevant studies were identified through searches conducted in the MEDLINE, PubMed, Scopus, Cochrane Central Register of Controlled Trials, IEEE Xplore, ACM Digital Library, and Open Grey databases from mid-June to August 2021. Studies were included if they used or evaluated chatbots for the purpose of prevention or intervention and for which the evidence showed a demonstrable health impact.

Results

Of the 1506 studies identified, 32 were included in the review. The results show a substantial increase in the interest of chatbots in the past few years, shortly before the pandemic. Half (16/32, 50%) of the research evaluated chatbots applied to mental health or COVID-19. The studies suggest promise in the application of chatbots, especially to easily automated and repetitive tasks, but overall, the evidence for the efficacy of chatbots for prevention and intervention across all domains is limited at present.

Conclusions

More research is needed to fully understand the effectiveness of using chatbots in public health. Concerns with the clinical, legal, and ethical aspects of the use of chatbots for health care are well founded given the speed with which they have been adopted in practice. Future research on their use should address these concerns through the development of expertise and best practices specific to public health, including a greater focus on user experience.
Home-Based Respiratory Physiotherapy and Telephone-Based Psychological Support for COVID-19 Survivors in Peru: Protocol for a Randomized Controlled Trial
Authors: Soriano-Moreno Anderson N , Flores Elaine C , Hartinger Stella M , Mallma Claudia Y , Diaz Arnold A , Gianella Gonzalo E , Galvez-Buccollini Juan A , Coico-Lama Abdiel H , Malaga German , Fajardo Eufemia , Paredes-Angeles Rubí , Otazú-Alfaro Sharlyn , Lescano Andres G , Checkley William ,   WAYRA Trial Investigators
Published: August 2022

Background

Both pulmonary and mental health are affected following hospitalization for COVID-19 pneumonia. Pulmonary rehabilitation therapy has demonstrated benefits in improving mental health, but no validated combined programs that include mental health have been proposed.

Objective

This article presents the design of a trial that aimed to assess whether the participation in a combined rehabilitation program that includes home-based respiratory physiotherapy and telephone-based psychological support is associated with a greater improvement of pulmonary and mental health outcomes 7-12 weeks after COVID-19 hospitalization discharge compared with posthospital usual care provided by a public Peruvian hospital.

Methods

WAYRA (the word for air in the Quechua language) was an open-label, unblinded, two-arm randomized controlled trial. We recruited 108 participants aged 18-75 years who were discharged from the hospital after COVID-19 pneumonia that required >6 liters/minute of supplemental oxygen during treatment. Participants were randomly assigned at a 1:1 ratio to receive the combined rehabilitation program or usual posthospital care provided by a public Peruvian hospital. The intervention consisted of 12 at-home respiratory rehabilitation sessions and 6 telephone-based psychological sessions. The primary outcome was the 6-minute walk distance. Secondary outcomes included lung function, mental health status (depression, anxiety, and trauma), and quality of life. Outcomes were assessed at baseline (before randomization) and at 7 and 12 weeks after hospital discharge to assess the difference between arms.

Results

This study was funded by the Peruvian National Council of Science Technology and Technology Innovation in July 2020. Ethics approval was obtained on September 2, 2020. Recruitment and data collection occurred between October 2020 and June 2021. Results are expected to be published by the end of 2022.

Conclusions

WAYRA was the first randomized controlled trial evaluating combined pulmonary-mental health rehabilitation for hospitalized COVID-19 survivors in resource-limited settings, potentially providing a foundation for the cost-effective scale-up of similar multidisciplinary rehabilitation programs.

Trial Registration

ClinicalTrials.gov NCT04649736; https://clinicaltrials.gov/ct2/show/NCT04649736

International Registered Report Identifier (IRRID)

RR1-10.2196/36001
Answering Hospital Caregivers’ Questions at Any Time: Proof-of-Concept Study of an Artificial Intelligence–Based Chatbot in a French Hospital
Authors: Daniel Thomas , de Chevigny Alix , Champrigaud Adeline , Valette Julie , Sitbon Marine , Jardin Meryam , Chevalier Delphine , Renet Sophie
Published: August 2022

Background

Access to accurate information in health care is a key point for caregivers to avoid medication errors, especially with the reorganization of staff and drug circuits during health crises such as the COVID‑19 pandemic. It is, therefore, the role of the hospital pharmacy to answer caregivers’ questions. Some may require the expertise of a pharmacist, some should be answered by pharmacy technicians, but others are simple and redundant, and automated responses may be provided.

Objective

We aimed at developing and implementing a chatbot to answer questions from hospital caregivers about drugs and pharmacy organization 24 hours a day and to evaluate this tool.

Methods

The ADDIE (Analysis, Design, Development, Implementation, and Evaluation) model was used by a multiprofessional team composed of 3 hospital pharmacists, 2 members of the Innovation and Transformation Department, and the IT service provider. Based on an analysis of the caregivers’ needs about drugs and pharmacy organization, we designed and developed a chatbot. The tool was then evaluated before its implementation into the hospital intranet. Its relevance and conversations with testers were monitored via the IT provider’s back office.

Results

Needs analysis with 5 hospital pharmacists and 33 caregivers from 5 health services allowed us to identify 7 themes about drugs and pharmacy organization (such as opening hours and specific prescriptions). After a year of chatbot design and development, the test version obtained good evaluation scores: its speed was rated 8.2 out of 10, usability 8.1 out of 10, and appearance 7.5 out of 10. Testers were generally satisfied (70%) and were hoping for the content to be enhanced.

Conclusions

The chatbot seems to be a relevant tool for hospital caregivers, helping them obtain reliable and verified information they need on drugs and pharmacy organization. In the context of significant mobility of nursing staff during the health crisis due to the COVID-19 pandemic, the chatbot could be a suitable tool for transmitting relevant information related to drug circuits or specific procedures. To our knowledge, this is the first time that such a tool has been designed for caregivers. Its development further continued by means of tests conducted with other users such as pharmacy technicians and via the integration of additional data before the implementation on the 2 hospital sites.
Partisipasi Anggota Kelompok Wanita Tani (KWT) Dalam Kegiatan Pekarangan Pangan Lestari Mendukung Ketahanan Pangan Rumah Tangga Di Kabupaten Gunungkidul DI Yogyakarta
Authors: Pratama Dian , Witjaksono Roso , Raya Alia Bihrajihant
Published: August 2022

ABSTRACTThe conversion of agricultural land that was still happening caused a decrease in land for food production. Decrease in food production could reduce food availability so that it became a threat to food resilience. The Ministry of Agriculture in 2020 launched the Sustainable Food Garden or Pekarangan Pangan Lestari (P2L) activity which aimed to increased availability, accessibility, and utilization of food. The success of P2L activities was determined by the participation of farmer women’s group members or Kelompok Wanita Tani (KWT) members in these activities. The objectives of the study were to: (1) Describing the participation of farmer women’s group members in P2L activities, and (2) Describing the effect of farmer women’s group members participation in P2L activities on household food resilience.This research was carried out with 6 KWT samples of beneficiaries of P2L activities in 2020 in Gunungkidul Regency which were taken by stratified cluster sampling. Primary data collection was done by direct interviews using a questionnaire to 138 respondents from KWT members taken by random sampling from 6 KWT samples. Data analysis had been done by using descriptive statistic and simple linear regression.The results showed that KWT members often and very often participated of attendance, but KWT members never and rarely participated of money and new ideas in P2L activities. The results of simple linear regression analysis showed that the participation of KWT members had a significant effect on household food resilience, namely the more often the participation of KWT members in P2L activities, the household food resilience of KWT members increases.