bluuebunny/crossref_metadata_2025 huggingface dataset:
BITOUR: A Business Intelligence Platform for Tourism Analysis
Authors: Bustamante Alexander , Sebastia Laura , Onaindia Eva
Published: November 2020

Integrating collaborative data in data-driven Business Intelligence (BI) system brings an opportunity to foster the decision-making process towards improving tourism competitiveness. This article presents BITOUR, a BI platform that integrates four collaborative data sources (Twitter, Openstreetmap, Tripadvisor and Airbnb). BITOUR follows a classical BI architecture and provides functionalities for data transformation, data processing, data analysis and data visualization. At the core of the data processing, BITOUR offers mechanisms to identify tourists in Twitter, assign tweets to attractions and accommodation sites from Tripadvisor and Airbnb, analyze sentiments in opinions issued by tourists, and all this using geolocation objects in Openstreetmap. With all these ingredients, BITOUR enables data analysis and visualization to answer questions like the most frequented places by tourists, the average stay length or the view of visitors of some particular destination.
Regulation of T-cell Receptor Gene Expression by Three-Dimensional Locus Conformation and Enhancer Function
Authors: Rodríguez-Caparrós Alonso , Álvarez-Santiago Jesús , del Valle-Pastor María Jesús , Suñé Carlos , López-Ros Jennifer , Hernández-Munain Cristina
Published: November 2020

The adaptive immune response in vertebrates depends on the expression of antigen-specific receptors in lymphocytes. T-cell receptor (TCR) gene expression is exquisitely regulated during thymocyte development to drive the generation of αβ and γδ T lymphocytes. The TCRα, TCRβ, TCRγ, and TCRδ genes exist in two different configurations, unrearranged and rearranged. A correctly rearranged configuration is required for expression of a functional TCR chain. TCRs can take the form of one of three possible heterodimers, pre-TCR, TCRαβ, or TCRγδ which drive thymocyte maturation into αβ or γδ T lymphocytes. To pass from an unrearranged to a rearranged configuration, global and local three dimensional (3D) chromatin changes must occur during thymocyte development to regulate gene segment accessibility for V(D)J recombination. During this process, enhancers play a critical role by modifying the chromatin conformation and triggering noncoding germline transcription that promotes the recruitment of the recombination machinery. The different signaling that thymocytes receive during their development controls enhancer activity. Here, we summarize the dynamics of long-distance interactions established through chromatin regulatory elements that drive transcription and V(D)J recombination and how different signaling pathways are orchestrated to regulate the activity of enhancers to precisely control TCR gene expression during T-cell maturation.
Analysis of miR-9-5p, miR-124-3p, miR-21-5p, miR-138-5p, and miR-1-3p in Glioblastoma Cell Lines and Extracellular Vesicles
Authors: Zottel Alja , Šamec Neja , Kump Ana , Raspor Dall’Olio Lucija Raspor , Pužar Dominkuš Pia , Romih Rok , Hudoklin Samo , Mlakar Jernej , Nikitin Daniil , Sorokin Maxim , Buzdin Anton , Jovčevska Ivana , Komel Radovan
Published: November 2020

Glioblastoma (GBM), the most common primary brain tumor, is a complex and extremely aggressive disease. Despite recent advances in molecular biology, there is a lack of biomarkers, which would improve GBM’s diagnosis, prognosis, and therapy. Here, we analyzed by qPCR the expression levels of a set of miRNAs in GBM and lower-grade glioma human tissue samples and performed a survival analysis in silico. We then determined the expression of same miRNAs and their selected target mRNAs in small extracellular vesicles (sEVs) of GBM cell lines. We showed that the expression of miR-21-5p was significantly increased in GBM tissue compared to lower-grade glioma and reference brain tissue, while miR-124-3p and miR-138-5p were overexpressed in reference brain tissue compared to GBM. We also demonstrated that miR-9-5p and miR-124-3p were overexpressed in the sEVs of GBM stem cell lines (NCH421k or NCH644, respectively) compared to the sEVs of all other GBM cell lines and astrocytes. VIM mRNA, a target of miR-124-3p and miR-138-5p, was overexpressed in the sEVs of U251 and U87 GBM cell lines compared to the sEVs of GBM stem cell line and also astrocytes. Our results suggest VIM mRNA, miR-9-5p miRNA, and miR-124-3p miRNA could serve as biomarkers of the sEVs of GBM cells.
Design, Synthesis, and In Vitro/In Vivo Anti-Cancer Activities of Novel (20S)-10,11-Methylenedioxy-Camptothecin Heterocyclic Derivatives
Authors: Dai Xiufen , Wu Guanzhao , Zhang Yixuan , Zhang Xiaomin , Yin Ruijuan , Qi Xin , Li Jing , Jiang Tao
Published: November 2020

A novel camptothecin analogue, (20S)-10,11-methylenedioxy-camptothecin (FL118), has been proven to show significant antitumor efficacy for a wide variety of solid tumors. However, the further development of FL118 is severely hindered due to its extremely poor water solubility and adverse side effects. Here, two series of novel 20-substituted (20S)-10,11-methylenedioxy-camptothecin coupled with 5-substituted uracils and other heterocyclic rings through glycine were synthesized. All the derivatives showed superior cytotoxic activities in vitro with IC50 values in the nanomolar range. Among them, 12e displayed higher cytotoxic activities in several cancer cell lines with better water solubility than FL118. Our results further showed that, like FL118, 12e inhibited cell proliferation resulting from cell cycle arrest and apoptosis by blocking the anti-apoptotic gene transcription of survivin, Mcl-1, Bcl-2, and XIAP in both A549 cells and NCI-H446 cells. Furthermore, 12e did not show any inhibitory activity on Topo I, which is involved in hematopoietic toxicity. In vivo, 12e showed similar antitumor efficacy to FL118 but lower toxicity. Our findings indicate that 12e is a promising therapeutic agent for cancer treatment, and the core structure of FL118 represents a promising platform to generate novel FL118-based antitumor drugs.
Roles of Non-Coding RNAs in Response to Nitrogen Availability in Plants
Authors: Fukuda Makiha , Fujiwara Toru , Nishida Sho
Published: November 2020

Nitrogen (N) is an essential nutrient for plant growth and development; therefore, N deficiency is a major limiting factor in crop production. Plants have evolved mechanisms to cope with N deficiency, and the role of protein-coding genes in these mechanisms has been well studied. In the last decades, regulatory non-coding RNAs (ncRNAs), such as microRNAs (miRNAs), small interfering RNAs (siRNAs), and long ncRNAs (lncRNAs), have emerged as important regulators of gene expression in diverse biological processes. Recent advances in technologies for transcriptome analysis have enabled identification of N-responsive ncRNAs on a genome-wide scale. Characterization of these ncRNAs is expected to improve our understanding of the gene regulatory mechanisms of N response. In this review, we highlight recent progress in identification and characterization of N-responsive ncRNAs in Arabidopsis thaliana and several other plant species including maize, rice, and Populus.
A Subset of Purposeless Oral Movements Triggered by Dopaminergic Agonists Is Modulated by 5-HT2C Receptors in Rats: Implication of the Subthalamic Nucleus
Authors: Lagière Mélanie , Bosc Marion , Whitestone Sara , Benazzouz Abdelhamid , Chagraoui Abdeslam , Millan Mark J. , De Deurwaerdère Philippe
Published: November 2020

Dopaminergic medication for Parkinson’s disease is associated with troubling dystonia and dyskinesia and, in rodents, dopaminergic agonists likewise induce a variety of orofacial motor responses, certain of which are mimicked by serotonin2C (5-HT2C) receptor agonists. However, the neural substrates underlying these communalities and their interrelationship remain unclear. In Sprague-Dawley rats, the dopaminergic agonist, apomorphine (0.03–0.3 mg/kg) and the preferential D2/3 receptor agonist quinpirole (0.2–0.5 mg/kg), induced purposeless oral movements (chewing, jaw tremor, tongue darting). The 5-HT2C receptor antagonist 5-methyl-1-[[2-[(2-methyl-3-pyridyl)oxyl]-5-pyridyl]carbamoyl]-6-trifluoromethylindone (SB 243213) (1 mg/kg) reduced the oral responses elicited by specific doses of both agonists (0.1 mg/kg apomorphine; 0.5 mg/kg quinpirole). After having confirmed that the oral bouts induced by quinpirole 0.5 mg/kg were blocked by another 5-HT2C antagonist (6-chloro-5-methyl-1-[6-(2-methylpiridin-3-yloxy)pyridine-3-yl carbamoyl] indoline (SB 242084), 1 mg/kg), we mapped the changes in neuronal activity in numerous sub-territories of the basal ganglia using c-Fos expression. We found a marked increase of c-Fos expression in the subthalamic nucleus (STN) in combining quinpirole (0.5 mg/kg) with either SB 243213 or SB 242084. In a parallel set of electrophysiological experiments, the same combination of SB 243213/quinpirole produced an irregular pattern of discharge and an increase in the firing rate of STN neurons. Finally, it was shown that upon the electrical stimulation of the anterior cingulate cortex, quinpirole (0.5 mg/kg) increased the response of substantia nigra pars reticulata neurons corresponding to activation of the “hyperdirect” (cortico-subthalamonigral) pathway. This effect of quinpirole was abolished by the two 5-HT2C antagonists. Collectively, these results suggest that induction of orofacial motor responses by D2/3 receptor stimulation involves 5-HT2C receptor-mediated activation of the STN by recruitment of the hyperdirect (cortico-subthalamonigral) pathway.
Combined Effects of Confinement and Macromolecular Crowding on Protein Stability
Authors: Ross Murial L. , Kunkel Jeffrey , Long Steven , Asuri Prashanth
Published: November 2020

Confinement and crowding have been shown to affect protein fates, including folding, functional stability, and their interactions with self and other proteins. Using both theoretical and experimental studies, researchers have established the independent effects of confinement or crowding, but only a few studies have explored their effects in combination; therefore, their combined impact on protein fates is still relatively unknown. Here, we investigated the combined effects of confinement and crowding on protein stability using the pores of agarose hydrogels as a confining agent and the biopolymer, dextran, as a crowding agent. The addition of dextran further stabilized the enzymes encapsulated in agarose; moreover, the observed increases in enhancements (due to the addition of dextran) exceeded the sum of the individual enhancements due to confinement and crowding. These results suggest that even though confinement and crowding may behave differently in how they influence protein fates, these conditions may be combined to provide synergistic benefits for protein stabilization. In summary, our study demonstrated the successful use of polymer-based platforms to advance our understanding of how in vivo like environments impact protein function and structure.
CSF Biomarkers Reflecting Protein Pathology and Axonal Degeneration Are Associated with Memory, Attentional, and Executive Functioning in Early-Stage Parkinson′s Disease
Authors: Oosterveld Linda P. , Kuiper Tessa I. , Majbour Nour K. , Verberk Inge M. W. , van Dijk Karin D. , Twisk Jos W. R. , El-Agnaf Omar M. , Teunissen Charlotte E. , Weinstein Henry C. , Klein Martin , Berendse Henk W. , van de Berg Wilma D. J.
Published: November 2020

In early-stage Parkinson′s disease (PD), cognitive impairment is common, and a variety of cognitive domains including memory, attention, and executive functioning may be affected. Cerebrospinal fluid (CSF) biomarkers are potential markers of cognitive functioning. We aimed to explore whether CSF α-synuclein species, neurofilament light chain, amyloid-β42, and tau are associated with cognitive performance in early-stage PD patients. CSF levels of total-α-synuclein and phosphorylated-α-synuclein, neurofilament light chain, amyloid-β42, and total-tau and phosphorylated-tau were measured in 26 PD patients (disease duration ≤5 years and Hoehn and Yahr stage 1–2.5). Multivariable linear regression models, adjusted for age, gender, and educational level, were used to assess the relationship between CSF biomarker levels and memory, attention, executive and visuospatial function, and language performance scores. In 26 early-stage PD patients, attention and memory were the most commonly affected domains. A higher CSF phosphorylated-α-synuclein/total-α-synuclein ratio was associated with better executive functioning (sβ = 0.40). Higher CSF neurofilament light was associated with worse memory (sβ = −0.59), attentional (sβ = −0.32), and executive functioning (sβ = −0.35). Reduced CSF amyloid-β42 levels were associated with poorer attentional functioning (sβ = 0.35). Higher CSF phosphorylated-tau was associated with worse language functioning (sβ = −0.33). Thus, CSF biomarker levels, in particular neurofilament light, were related to the most commonly affected cognitive domains in early-stage PD. This indicates that CSF biomarker levels may identify early-stage PD patients who are at an increased risk of developing cognitive impairment.
Impact of Trail Running Races on Blood Viscosity and Its Determinants: Effects of Distance
Authors: Robert Mélanie , Stauffer Emeric , Nader Elie , Skinner Sarah , Boisson Camille , Cibiel Agnes , Feasson Léonard , Renoux Céline , Robach Paul , Joly Philippe , Millet Guillaume Y. , Connes Philippe
Published: November 2020

Blood rheology is a key determinant of tissue perfusion at rest and during exercise. The present study investigated the effects of race distance on hematological, blood rheological, and red blood cell (RBC) senescence parameters. Eleven runners participated in the Martigny–Combes à Chamonix 40 km race (MCC, elevation gain: 2300 m) and 12 others in the Ultra-Trail du Mont Blanc (UTMB, 171 km, elevation gain: 10,000 m). Blood samples were collected before and after the races. After the UTMB, the percentage of RBC phosphatidylserine (PS) exposure was not affected while RBC CD235a levels decreased and RBC-derived microparticles increased. In contrast, after the MCC, RBC PS exposure increased, while RBC CD235a and RBC-derived microparticles levels were not affected. The free hemoglobin and hemolysis rate did not change during the races. RBC aggregation and blood viscosity at moderate shear rates increased after the MCC. RBC deformability, blood viscosity at a high shear rate, and hematocrit decreased after the UTMB but not after the MCC. Our results indicate that blood rheology behavior is different between a 40 km and a 171 km mountain race. The low blood viscosity after the ultra-marathon might facilitate blood flow to the muscles and optimize aerobic performance.
Management of Brain and Leptomeningeal Metastases from Breast Cancer
Authors: Pellerino Alessia , Internò Valeria , Mo Francesca , Franchino Federica , Soffietti Riccardo , Rudà Roberta
Published: November 2020

The management of breast cancer (BC) has rapidly evolved in the last 20 years. The improvement of systemic therapy allows a remarkable control of extracranial disease. However, brain (BM) and leptomeningeal metastases (LM) are frequent complications of advanced BC and represent a challenging issue for clinicians. Some prognostic scales designed for metastatic BC have been employed to select fit patients for adequate therapy and enrollment in clinical trials. Different systemic drugs, such as targeted therapies with either monoclonal antibodies or small tyrosine kinase molecules, or modified chemotherapeutic agents are under investigation. Major aims are to improve the penetration of active drugs through the blood–brain barrier (BBB) or brain–tumor barrier (BTB), and establish the best sequence and timing of radiotherapy and systemic therapy to avoid neurocognitive impairment. Moreover, pharmacologic prevention is a new concept driven by the efficacy of targeted agents on macrometastases from specific molecular subgroups. This review aims to provide an overview of the clinical and molecular factors involved in the selection of patients for local and/or systemic therapy, as well as the results of clinical trials on advanced BC. Moreover, insight on promising therapeutic options and potential directions of future therapeutic targets against BBB and microenvironment are discussed.
A Biochemical Platform to Define the Relative Specific Activity of IDUA Variants Identified by Newborn Screening
Authors: Yu Seok-Ho , Pollard Laura , Wood Tim , Flanagan-Steet Heather , Steet Richard
Published: November 2020

The lysosomal storage disorder, mucopolysaccharidosis I (MPSI), results from mutations in IDUA, the gene that encodes the glycosaminoglycan-degrading enzyme α-L-iduronidase. Newborn screening efforts for MPSI have greatly increased the number of novel IDUA variants identified, but with insufficient experimental evidence regarding their pathogenicity, many of these variants remain classified as variants of uncertain significance (VUS). Defining pathogenicity for novel IDUA variants is critical for decisions regarding medical management and early intervention. Here, we describe a biochemical platform for the characterization of IDUA variants that relies on viral delivery of IDUA DNA into IDUA-deficient HAP1 cells and isolation of single cell expression clones. The relative specific activity of wild-type and variant α-iduronidase was determined using a combination of Western blot analysis and α-iduronidase activity assays. The specific activity of each variant enzyme was consistent across different single cell clones despite variable IDUA expression and could be accurately determined down to 0.05–0.01% of WT α-iduronidase activity. With this strategy we compared the specific activities of known pseudodeficiency variants (p.His82Gln, p.Ala79Thr, p.Val322Glu, p.Asp223Asn) or pathogenic variants (p.Ser633Leu, p.His240Arg) with variants of uncertain significance (p.Ser586Phe, p.Ile272Leu). The p.Ser633Leu and p.His240Arg variants both show very low activities consistent with their association with Scheie syndrome. In our experiments, however, p.His240Arg exhibited a specific activity five times higher than p.Ser633Leu in contrast to other reports showing equivalent activity. Cell clones expressing the p.Ser586Phe and p.Ile272Leu variants had specific activities in the range of other pseudodeficiency variants tested. Our findings show that pseudodeficiency and pathogenic variants can be distinguished from each other with regard to specific activity, and confirms that all the pseudodeficiency variants variably reduce α-iduronidase activity. We envision this platform will be a valuable resource for the rigorous assessment of the novel IDUA variants emerging from the expansion of newborn screening efforts.
Automated Construction Progress and Quality Monitoring for Commercial Buildings with Unmanned Aerial Systems: An Application Study from Switzerland
Authors: Kielhauser Clemens , Renteria Manzano Raul , Hoffman Jeffrey J. , Adey Bryan T.
Published: November 2020

Unmanned Aerial Systems (UASs), which have become a key tool in remote sensing in recent years, have also rapidly entered the practice of Architecture, Engineering, Construction, and Operations (AECO). This paper presents an application study of a methodology for monitoring construction progress and quality using real-time data from a commercial building during the execution phase and the results of an analysis of the costs and benefits of monitoring construction progress and quality with and without a UAS. The methodology used consists of three parts: (1) automated data collection at the construction site, (2) data processing, in which the collected data are processed to generate the outputs necessary for the data analysis, and (3) data analysis to monitor construction progress and quality. The method is based on the detection of structural elements, combined with four principles: the sectional approach, the calculation of the concrete volume, the height-distance measurement, and the detection of defects by visual comparisons. The cost-benefit analysis considers three cases: monitoring of progress (1) by the construction company using the UAS, (2) by external contractors using the UAS, and (3) by the construction company without using the UAS. We show that the in-house operation of the UAS is associated with medium costs. However, a return on investment can be achieved quickly if the strategy for the operation of the UAS is clearly defined. In summary, the use of a UAS for the automated monitoring of the construction progress and quality of commercial buildings is practicable, which can quickly lead to a return on investment. We also show that there is great potential for further improvements.
Development of a Tet-On Inducible Expression System for the Anhydrobiotic Cell Line, Pv11
Authors: Tokumoto Shoko , Miyata Yugo , Usui Kengo , Deviatiiarov Ruslan , Ohkawa Takahiro , Kondratieva Sabina , Shagimardanova Elena , Gusev Oleg , Cornette Richard , Itoh Masayoshi , Hayashizaki Yoshihide , Kikawada Takahiro
Published: November 2020

The Pv11 cell line established from an African chironomid, Polypedilum vanderplanki, is the only cell line tolerant to complete desiccation. In Pv11 cells, a constitutive expression system for Pv11 cells was previously exploited and several reporter genes were successfully expressed. Here we report the identification of an effective minimal promoter for Pv11 cells and its application to the Tet-On inducible expression system. First, using a luciferase reporter assay, we showed that a 202 bp deletion fragment derived from the constitutively active 121-promoter functions in Pv11 cells as an appropriate minimal promoter with the Tet-On inducible expression system. The AcGFP1 (Aequorea coerulescens green fluorescent protein) was also successfully expressed in Pv11 cells using the inducible system. In addition to these reporter genes, the avian myeloblastosis virus reverse transcriptase α subunit (AMV RTα), which is one of the most widely commercially available RNA-dependent DNA polymerases, was successfully expressed through the inducible expression system and its catalytic activity was verified. These results demonstrate the establishment of an inducible expression system in cells that can be preserved in the dry state and highlight a possible application to the production of large and complex proteins.
Culturing-Enriched Metabarcoding Analysis of the Oryctes rhinoceros Gut Microbiome
Authors: Shelomi Matan , Chen Ming-Ju
Published: November 2020

Wood-feeding insects should have a source of enzymes like cellulases to digest their food. These enzymes can be produced by the insect, or by microbes living in the wood and/or inside the insect gut. The coconut rhinoceros beetle, Oryctes rhinoceros, is a pest whose digestive microbes are of considerable interest. This study describes the compartments of the O. rhinoceros gut and compares their microbiomes using culturing-enriched metabarcoding. Beetle larvae were collected from a coconut grove in southern Taiwan. Gut contents from the midgut and hindgut were plated on nutrient agar and selective carboxymethylcellulose agar plates. DNA was extracted from gut and fat body samples and 16S rDNA metabarcoding performed to identify unculturable bacteria. Cellulase activity tests were performed on gut fluids and microbe isolates. The midgut and hindgut both showed cellulolytic activity. Bacillus cereus, Citrobacter koseri, and the cellulolytic fungus Candida xylanilytica were cultured from both gut sections in most larvae. Metabarcoding did not find Bacillus cereus, and found that either Citrobacter koseri or Paracoccus sp. were the dominant gut microbes in any given larva. No significant differences were found between midgut and hindgut microbiomes. Bacillus cereus and Citrobacter koseri are common animal gut microbes frequently found in Oryctes rhinoceros studies while Candida xylanilytica and the uncultured Paracoccus sp. had not been identified in this insect before. Some or all of these may well have digestive functions for the beetle, and are most likely acquired from the diet, meaning they may be transient commensalists rather than obligate mutualists. Broader collection efforts and tests with antibiotics will resolve ambiguities in the beetle–microbe interactions.
Impact of the Invasive Argentine Ant in Citrus Agroecosystems: Effects on the Diversity and Frequency of Native Ant Species Foraging on Tree Canopy
Authors: Zina Vera , Branco Manuela , Franco José Carlos
Published: November 2020

The invasion of the Argentine ant, Linepithema humile (Mayr) (Hymenoptera, Formicidae) can alter the entire ecosystem with serious impacts on the native community structure (e.g., ant diversity) and processes (e.g., trophic interactions) leading to biodiversity loss and pest outbreaks. Most studies addressing these impacts have been conducted in natural or semi-natural areas, few are those conducted in agricultural ecosystems, such as citrus orchards. These are dominant agricultural ecosystems in Mediterranean landscapes. Furthermore, most studies have been conducted in a short span, not evidencing seasonal fluctuations. In this work, we assessed the ecological impact of the Argentine ant on the native ant communities in citrus orchards, in the region of Algarve, southern Portugal. By using principal response curve, we compared seasonal variation on ant assemblages in invaded and uninvaded citrus orchards foraging on tree canopy from a two-year sampling. The Argentine ant had a marked negative impact on the native ant community foraging on citrus canopy. In the uninvaded orchards, the native ant community had a rich assemblage composed of 16 ant species, in its majority (72%) controlled by the dominant species Lasius grandis Forel, Tapinoma nigerrimum (Nylander) and/or Pheidole pallidula (Nylander). In the invaded orchards, the native ant community was poorer and highly modified, mostly dominated by the Argentine ant (80%). Apparently, the only native ant species not affected by the presence of the Argentine ant was Plagiolepis pygmaea (Latreille). A significant negative effect was found between the proportion of infested trees by L. humile and the number of native ant species per orchard. Differences in the native ant community in the invaded and uninvaded orchards persisted over seasons and years. However, negative impacts were higher in the spring and summer, and less pronounced in the autumn. We discuss implications for citrus pest management.
RNA Interference-Based Silencing of the Chitin Synthase 1 Gene for Reproductive and Developmental Disruptions in Panonychus citri
Authors: Ali Muhammad Waqar , Khan Muhammad Musa , Song Fang , Wu Liming , He Ligang , Wang Zhijing , Zhang Zhen-yu , Zhang Hongyu , Jiang Yingchun
Published: November 2020

Chitin synthase 1 (CHS1) is an essential gene regulating chitin during different developmental stages of arthropods. In the current study, we explored for the first time the role of CHS1 gene regulation in the citrus red mite, Panonychus citri (McGregor) (Acari: Tetranychidae), by silencing its expression using (RNA interference) RNAi-based strategies. The results reveal that P. citri tested in different developmental stages, including larvae, protonymphs, deutonymphs, and adults fed on sweet orange leaves dipped in various concentrations (200, 400, 600, and 800 ng/μL) of dsRNA-PcCHS1, resulted in a continuous reduction in their gene expression, and the extent of transcript knockdown was positively correlated with the concentration of dsRNA. Concentration–mortality response assays revealed a mortality of more than 50% among all the studied developmental stages, except for adulthood. Furthermore, the target gene dsRNA-PcCHS1 treatment of larvae, protonymphs, deutonymphs, and females at a treatment rate of 800 ng/mL of dsRNA significantly decreased the egg-laying rates by 48.50%, 43.79%, 54%, and 39%, respectively, and the hatching rates were also considerably reduced by 64.70%, 70%, 64%, and 52.90%, respectively. Moreover, using the leaf dip method, we found that the RNA interference effectively reduced the PcCHS1 transcript levels by 42.50% and 42.06% in the eggs and adults, respectively. The results of this study demonstrate that the RNAi of PcCHS1 can dramatically reduce the survival and fecundity of P. citri, but the dsRNA concentrations and developmental stages can significantly influence the RNAi effects. These findings indicate the potential utility of the PcCHS1 gene in causing developmental irregularities, which could aid in the development of effective and novel RNAi-based strategies for controlling P. citri.
Comparison of Toxicological Bioassays for Whiteflies
Authors: Sparks Tanner C. , Riley David G. , Simmons Alvin M. , Guo Liangzhen
Published: November 2020

Two Bemisia tabaci populations from Georgia and Florida, USA, were tested for their response to insecticides across different toxicological bioassay methods. Five insecticides in four Insecticide Resistance Action Committee (IRAC) groups (imidacloprid (4A), dinotefuran (4A), flupyradifurone (4D), pyriproxyfen (7C) and cyantraniliprole (28)), were evaluated against a water check. The routes of application to the plant used were either leaf drench or (systemic) root drench. The four different whitefly bioassay methodologies tested were two published IRAC methods, a clip cage method, and a new tube method. A split–split experimental design was used to assess any interactions between application route, bioassay method and insecticide treatment. Application route had no significant effect on efficacy. However, bioassay method affected overall whitefly mortality, with the dish method having reduced mortality compared to other methods, except for the clip cage method. High rates of cyantraniliprole, dinotefuran and flupyradifurone insecticides resulted in the highest incidence of adult whitefly mortality. Significant interactions relative to percent adult mortality were found between the insecticide and bioassay method for both populations assayed. The clip cage method was more sensitive in terms of dose mortality response followed by the cup and tube methods. The dish method was the least responsive to insecticide dose. Other interactions are discussed.
Prognostic Value of Elevated Copeptin and High-Sensitivity Cardiac Troponin T in Patients with and without Acute Coronary Syndrome: The ConTrACS Study
Authors: Waldsperger Hanna , Biener Moritz , Stoyanov Kiril M. , Vafaie Mehrshad , Katus Hugo A. , Giannitsis Evangelos , Mueller-Hennessen Matthias
Published: November 2020

Aims: We aimed to assess the prognostic role of copeptin in patients presenting to the emergency department with acute symptoms and increased high-sensitivity cardiac troponin T. Methods: A total of 3890 patients presenting with acute symptoms to the emergency department of Heidelberg University Hospital were assessed for increased hs-cTnT (>14 ng/L) from three cohorts: the Heidelberg Acute Coronary Syndrome (ACS) Registry (n = 2477), the BIOPS Registry (n = 320), and the ACS OMICS Registry (n = 1093). In a pooled analysis, 1956 patients remained, comprising of 1600 patients with ACS and 356 patients with non-ACS. Results: Median follow-up was 1468 days in the ACS cohort and 709 days in the non-ACS cohort. Elevated copeptin levels (>10 pmol/L) were found in 1174 patients (60.0%) in the entire cohort (58.1% in ACS and 68.5% in non-ACS, respectively) and mortality rates were significantly higher than in patients with normal copeptin levels (29.0% vs. 10.7%, p < 0.001). In a multivariate Cox regression, elevated copeptin was independently associated with all-cause death in the ACS (HR = 1.7, 1.3–2.3, p = 0.002) and non-ACS cohort (HR = 2.7, 1.4–5.0, p = 0.0018). Conclusion: Copeptin may aid in identifying patients at risk for adverse outcomes in patients with increased levels of hs-cTnT in ACS patients and in non-ACS conditions.
Effect of Excessive Body Weight and Psoriasis in Women Undergoing ICSI Procedure and State of Health of the Newborn
Authors: Wdowiak-Filip Anita , Wdowiak Artur , Raczkiewicz Dorota , Bartosińska Joanna , Bojar Iwona
Published: November 2020

Excessive body weight and some concomitant diseases, such as psoriasis, accompany women treated due to infertility by intracytoplasmic sperm injection (ICSI). This study is aimed to assess effect of obesity and psoriasis on quality of egg cells, embryos, course of pregnancy, and state of a newborn after treatment with ICSI. A total of 140 women were included into the study (110 healthy women and 30 with psoriasis). Among healthy women, BMI negatively correlated with total recovery rate, total oocyte score, blastocyst formation rate (BFR) and amount and quality of blastocysts (r < 0, p < 0.001). The relationships were similar in psoriasis, however apart from average blastocyst quality (p = 0.17) and BFR (p = 0.352). In healthy patients, BMI negatively correlated with gestational age at delivery (r = −0.444, p = 0.010) and APGAR (r = −0.481, p = 0.005). An excess of adipose tissue exerts an unfavourable effect on female reproductive functions, especially with a simultaneous burden of psoriasis. Excessive body weight is conducive to development of gestational diabetes and shortens the duration of pregnancy. The burden of psoriasis in combination with excessive body weight has an impact on the risk of occurrence of intrauterine growth restriction of foetus. Overweight and obesity negatively affect the state of a newborn, measured using APGAR scale.
Longitudinal Asthma Patterns in Italian Adult General Population Samples: Host and Environmental Risk Factors
Authors: Maio Sara , Baldacci Sandra , Simoni Marzia , Angino Anna , La Grutta Stefania , Muggeo Vito , Fasola Salvatore , Viegi Giovanni ,   on behalf of the AGAVE Pisa Group
Published: November 2020

Background: Asthma patterns are not well established in epidemiological studies. Aim: To assess asthma patterns and risk factors in an adult general population sample. Methods: In total, 452 individuals reporting asthma symptoms/diagnosis in previous surveys participated in the AGAVE survey (2011–2014). Latent transition analysis (LTA) was performed to detect baseline and 12-month follow-up asthma phenotypes and longitudinal patterns. Risk factors associated with longitudinal patterns were assessed through multinomial logistic regression. Results: LTA detected four longitudinal patterns: persistent asthma diagnosis with symptoms, 27.2%; persistent asthma diagnosis without symptoms, 4.6%; persistent asthma symptoms without diagnosis, 44.0%; and ex -asthma, 24.1%. The longitudinal patterns were differently associated with asthma comorbidities. Persistent asthma diagnosis with symptoms showed associations with passive smoke (OR 2.64, 95% CI 1.10–6.33) and traffic exposure (OR 1.86, 95% CI 1.02–3.38), while persistent asthma symptoms (without diagnosis) with passive smoke (OR 3.28, 95% CI 1.41–7.66) and active smoke (OR 6.24, 95% CI 2.68–14.51). Conclusions: LTA identified three cross-sectional phenotypes and their four longitudinal patterns in a real-life setting. The results highlight the necessity of a careful monitoring of exposure to active/passive smoke and vehicular traffic, possible determinants of occurrence of asthma symptoms (with or without diagnosis). Such information could help affected patients and physicians in prevention and management strategies.
Enhanced Contactless Vital Sign Estimation from Real-Time Multimodal 3D Image Data
Authors: Zhang Chen , Gebhart Ingo , Kühmstedt Peter , Rosenberger Maik , Notni Gunther
Published: November 2020

The contactless estimation of vital signs using conventional color cameras and ambient light can be affected by motion artifacts and changes in ambient light. On both these problems, a multimodal 3D imaging system with an irritation-free controlled illumination was developed in this work. In this system, real-time 3D imaging was combined with multispectral and thermal imaging. Based on 3D image data, an efficient method was developed for the compensation of head motions, and novel approaches based on the use of 3D regions of interest were proposed for the estimation of various vital signs from multispectral and thermal video data. The developed imaging system and algorithms were demonstrated with test subjects, delivering a proof-of-concept.
A Reappraisal of the Threshold Hypothesis of Creativity and Intelligence
Authors: Weiss Selina , Steger Diana , Schroeders Ulrich , Wilhelm Oliver
Published: November 2020

Intelligence has been declared as a necessary but not sufficient condition for creativity, which was subsequently (erroneously) translated into the so-called threshold hypothesis. This hypothesis predicts a change in the correlation between creativity and intelligence at around 1.33 standard deviations above the population mean. A closer inspection of previous inconclusive results suggests that the heterogeneity is mostly due to the use of suboptimal data analytical procedures. Herein, we applied and compared three methods that allowed us to handle intelligence as a continuous variable. In more detail, we examined the threshold of the creativity-intelligence relation with (a) scatterplots and heteroscedasticity analysis, (b) segmented regression analysis, and (c) local structural equation models in two multivariate studies (N1 = 456; N2 = 438). We found no evidence for the threshold hypothesis of creativity across different analytical procedures in both studies. Given the problematic history of the threshold hypothesis and its unequivocal rejection with appropriate multivariate methods, we recommend the total abandonment of the threshold.
Evaluation of a Dynamic Bioremediation System for the Removal of Metal Ions and Toxic Dyes Using Sargassum Spp.
Authors: López-Miranda J. Luis , Silva Rodolfo , Molina Gustavo A. , Esparza Rodrigo , Hernandez-Martinez A. R. , Hernández-Carteño J. , Estévez Miriam
Published: November 2020

This work presents the results obtained in the design and manufacture of a simple, economic and ecological filter based on Sargassum spp. (Sspp), consisting of the species S. natans and S. fluitans, for the elimination of organic and inorganic toxic substances. The main objective is to make use of Sspp, as the massive amounts of this alga arriving at the Mexican Caribbean coast have caused serious problems over recent years. The toxic substances treated were organic dyes (methyl blue, methyl orange and methyl red) and the metal ion, lead (II). To obtain optimal removal conditions, grinding of the Sspp used, its mass and humidity were evaluated. In the design of the filter the area, flow rate and the number of layers were evaluated. Removal rates of almost 100%, 65% and 25% were obtained for methylene blue, methyl red and methyl orange respectively, and in the case of lead (II), values up to 95% were obtained. After the tests, the Sspp was characterized, using Fourier Transform Infrared (FTIR) spectroscopy and scanning electron microscopy, showing the presence of the dyes and the ionic species. These results demonstrate the efficiency of the dynamic Sspp-based filtration system proposed, which can be industrially scaled for the treatment of water contaminated with these kinds of substances.
Dynamic Load and Strength Determination of Carrying Structure of Wagons Transported by Ferries
Authors: Lovska Alyona , Fomin Oleksij , Píštěk Václav , Kučera Pavel
Published: November 2020

A rapid development of economic relations between states has required an introduction of combined rail/ferry transportation. A feature of this type of transportation is the possibility of wagons traveling by sea on specially equipped ships—railway ferries. However, the transportation of wagons by sea is accompanied by damage to their structures. This follows from the fact that the wagons are not adapted to this type of transportation. In addition, the design of wagons does not consider the loads that can act on them during transportation by sea. In this connection, it is important to study the dynamic loading and strength of wagons during their transportation by sea. The current work investigated into dynamic load on the carrying structure of wagons transported by ferries using mathematical and computer modelling and defined accelerations on the wagon body. The adequacy of the models was checked with an F-test. The results of strength calculation for the carrying structure of a wagon made it possible to conclude that a typical fixation diagram does not provide the admissible range of strength parameters. The current work proposed an improved fixation diagram for cars on the deck with the viscous binder. The solution was substantiated by the results of the mathematical modelling of dynamic forces, strength, and stability of the carrying structure of a wagon on the deck. The results of the work will contribute to the creation of recommendations for the transportation of wagons on ferries. The research can contribute to safe transportation of wagons by ferries, and higher efficiency of the combined transportation
Really Onychocellids? Revisions and New Findings Increase the Astonishing Bryozoan Diversity of the Mediterranean Sea
Authors: Rosso Antonietta , Gerovasileiou Vasilis , Di Martino Emanuela
Published: November 2020

Investigation of bryozoan faunas collected in two submarine caves in Lesvos Island, Aegean Sea revealed a great number of colonies of three species currently assigned to the cheilostome family Onychocellidae: Onychocella marioni Jullien, 1882, O. vibraculifera Neviani, 1895, and Smittipora disjuncta Canu & Bassler, 1930. All species were first described and subsequently recorded on several occasions, from the Mediterranean Sea, particularly from the Aegean Sea. The availability of this material provided the basis for more detailed observations and first scanning electron microscopy (SEM) study of some diagnostic characters, including ovicells and ancestrulae, for the well-known species, as well as a few colonies of a species left in open nomenclature (i.e., Onychocellidae sp. 1) in previous works. In this paper we (i) update the descriptions of these four species; (ii) resurrect the species Floridinella arculifera Canu & Bassler, 1927, which was previously synonymised with Caleschara minuta (Maplestone, 1909), suggesting for it the new combination Tretosina arculifera; (iii) and introduce the new genus Bryobifallax for S. disjuncta.
A New Experimental Study and SPH Comparison for the Sequential Dam-Break Problem
Authors: Kocaman Selahattin , Dal Kaan
Published: November 2020

The floods following the event of a dam collapse can have a significant impact on the downstream environment and ecology. Due to the limited number of real-case data for dam-break floods, laboratory experiments and numerical models are used to understand the complex flow behavior and to analyze the impact of the dam-break wave for different scenarios. In this study, a newly designed experimental campaign was conducted for the sequential dam-break problem in a rectangular channel with a steep slope, and the obtained results were compared against those of a particle-based numerical model. The laboratory tests permitted a better understanding of the physical process, highlighting five successive stages observed in the downstream reservoirs: dam-break wave propagation, overtopping, reflection wave, run-up, and oscillations. Experimental data were acquired using a virtual wave probe based on an image processing technique. A professional camera and a smartphone camera were used to obtain the footage of the experiment to examine the effect of the resolution and frame rate on image processing. The numerical results were obtained through the Smoothed Particle Hydrodynamics (SPH) method using free DualSPHysics software. The experimental and numerical results were in good agreement generally. Hence, the presented data can be used as a benchmark in future studies to validate the SPH and other Computational Fluid Dynamics (CFD) methods.
Posidonia oceanica as a Source of Chromophoric Dissolved Organic Matter for the Oligotrophic NW Mediterranean Coast
Authors: Iuculano Francesca , Duarte Carlos M. , Otero Jaime , Álvarez-Salgado Xosé Antón , Agustí Susana
Published: November 2020

Posidonia oceanica is a well-recognized source of dissolved organic matter (DOM) derived from exudation and leaching of seagrass leaves, but little is known about its impact on the chromophoric fraction of DOM (CDOM). In this study, we monitored for two years the optical properties of CDOM in two contrasting sites in the Mallorca Coast (Balearic Islands). One site was a rocky shore free of seagrass meadows, and the second site was characterized by the accumulation of non-living seagrass material in the form of banquettes. On average, the integrated color over the 250–600 nm range was almost 6-fold higher in the beach compared with the rocky shore. Furthermore, the shapes of the CDOM spectra in the two sites were also different. A short incubation experiment suggested that the spectral differences were due to leaching from P. oceanica leaf decomposition. Furthermore, occasionally the spectra of P. oceanica was distorted by a marked absorption increase at wavelength < 265 nm, presumably related to the release of hydrogen sulfide (HS−) associated with the anaerobic decomposition of seagrass leaves within the banquettes. Our results provide the first evidence that P. oceanica is a source of CDOM to the surrounding waters.
Performance of an Array of Oscillating Water Column Devices in Front of a Fixed Vertical Breakwater
Authors: Konispoliatis Dimitrios N.
Published: November 2020

The present study explores the performance of an array of cylindrical oscillating water column (OWC) devices, having a vertical symmetry axis, placed in front of a bottom seated, surface piercing, vertical breakwater. The main goal of this study is the investigation of a possible increase in the power efficiency of an OWC array by applying, in the vicinity of the array, a barrier to the wave propagation, aiming at amplifying the scattered and reflected waves originating from the presence of the devices and the wall. To cope with the set goal, a theoretical analysis is presented in the framework of linear potential theory, based on the solution of the proper diffraction, and pressure-radiation problems in the frequency domain, using the image theory, the matched axisymmetric eigenfunction expansion formulation, and the multiple scattering approach. Numerical results are presented and discussed in terms of the expected power absorption by the OWCs comparing different array’s characteristics i.e.,: (a) angle of incidence of the incoming wave train; (b) distances from the breakwater; and (c) geometric characteristics of the different arrangements. The results show that compared to the isolated OWC array (i.e., no presence of the wall), the power efficiency of the OWCs in front of a breakwater is amplified at specific frequency ranges.