bluuebunny/crossref_metadata_2025 huggingface dataset:
Exclusive Enteral Nutrition Orchestrates Immunological Balances as Early as Week 4 in Adult Patients of Crohn’s Disease: A Pilot, Open-Lable Study
Authors: Diao Na , Liu Xinyu , Lin Minzhi , Yang Qingfan , Li Bingyang , Tang Jian , Ding Ni , Gao Xiang , Chao Kang
Published: December 2023

Background and aims: The efficacy and underlying mechanisms of exclusive enteral nutrition (EEN) in adult patients with Crohn’s disease (CD) remain controversial. This study aimed to evaluate the role of EEN in adult patients with CD and to explore the mechanisms from the perspective of immunoregulation. Methods: This is a prospective, open-label pilot study. Active patients with CD were enrolled and prescribed an amino-acid-rich elemental diet for 12 weeks. Dynamic changes in immune cells, including neutrophils, monocytes, T cells and B cells, were detected by flow cytometry. Plasma cytokines were evaluated by ELISA. Results: Twenty adult patients with CD were enrolled. Among them, 1 discontinued treatment due to poor compliance, and 19 patients were included for final analysis. Clinical remission was achieved in 47.37% (9/19), 63.16% (12/19), and 73.68% (14/19) patients at weeks 4, 8, and 12, respectively. Endoscopic remission and transmural healing were achieved in 52.63% (10/19) and 15.79% (3/19) patients at week 12. Notably, there was no significant difference in clinical remission between week 4 and week 8 (p = 0.33) or week 12 (p = 0.09). Furthermore, we observed a rapid reconstitution of immunologic homeostasis as early as week 4. At week 4, both the frequency and activation of neutrophils and monocytes were decreased after EEN therapy. Significant decreases in Th17 cells and naïve B cells, increases in memory B cells, and regulatory B cells were also detected. These changes remained stable at weeks 8 and 12. Conclusions: EEN with an amino-acid-rich elemental diet orchestrated immunological balances and induces clinical remission in adult CD patients as early as week 4, suggesting a 4-week EEN therapy may be feasible and practicable in clinical practice.
A Population-Based Cross-Sectional Study of Paediatric Coeliac Disease in Catalonia Showed a Downward Trend in Prevalence Compared to the Previous Decade
Authors: Arau Beatriz , Dietl Beatriz , Sudrià-Lopez Emma , Ribes Josefa , Pareja Laura , Marquès Teresa , Garcia-Puig Roger , Pujalte Francisco , Martin-Cardona Albert , Fernández-Bañares Fernando , Mariné Meritxell , Farré Carme , Esteve Maria
Published: December 2023

(1) Background: Previous studies showed an increased prevalence and incidence of coeliac disease (CD) over time. The objective is to ascertain whether the CD prevalence in Catalonia (a region of Southern Europe) among children aged 1–5 is as high as previously found in 2004–2009; (2) Methods: From 2013 to 2019, 3659 subjects aged 1–5 years were recruited following the previously used methodology. Factors with a potential impact on CD prevalence were investigated; (3) Results: In 2013–2019, 43/3659 subjects had positive serology, giving a standardised seroprevalence of 12.55/1000 (95% CI: 8.92; 17.40), compared to 23.62 (13.21; 39.40) in 2004–2007. The biopsy-proven crude prevalence was 7.92/1000 (95% CI: 5.50; 11.30), and the crude prevalence based on ESPGHAN criteria was 8.74/1000 (95% CI: 6.20–12.30). In contrast to 2004–2009, we did not find differences in the seroprevalence rates between 1 and 2 years vs. 3 and 4 years of age (age percentage of change −7.0 (−29.5; 22.8) vs. −45.3 (−67.5; −8.0)). Rotavirus vaccination was the most remarkable potential protective factor (48% vs. 9% in 2004–2009; p < 0.0001), but not the time of gluten introduction. (4) Conclusion: The present study did not confirm a worldwide CD prevalence increase and emphasizes the need to perform prevalence studies over time using the same methodology in the same geographical areas.
An Overview of the Influence of Breastfeeding on the Development of Inflammatory Bowel Disease
Authors: Bertin Benjamin , Foligne Benoit , Ley Delphine , Lesage Jean , Beghin Laurent , Morcel Jules , Gottrand Frédéric , Hermann Emmanuel
Published: December 2023

The first 1000 days of life is a critical period that contributes significantly to the programming of an individual’s future health. Among the many changes that occur during this period early in life, there is growing evidence that the establishment of healthy gut microbiota plays an important role in the prevention of both short- and long-term health problems. Numerous publications suggest that the quality of the gut microbiota colonisation depends on several dietary factors, including breastfeeding. In this respect, a relationship between breastfeeding and the risk of inflammatory bowel disease (IBD) has been suggested. IBDs are chronic intestinal diseases, and perinatal factors may be partly responsible for their onset. We review the existence of links between breastfeeding and IBD based on experimental and clinical studies. Overall, despite encouraging experimental data in rodents, the association between breastfeeding and the development of IBD remains controversial in humans, partly due to the considerable heterogeneity between clinical studies. The duration of exclusive breastfeeding is probably decisive for its lasting effect on IBD. Thus, specific improvements in our knowledge could support dietary interventions targeting the gut microbiome, such as the early use of prebiotics, probiotics or postbiotics, in order to prevent the disease.
Extrapulmonary and Drug-Resistant Childhood Tuberculosis: Unveiling the Disease to Adopt the Optimal Treatment Strategy
Authors: Pace Domenico , Corvaglia Francesca , Lisi Catiuscia , Galli Luisa , Chiappini Elena
Published: December 2023

Paediatric tuberculosis (TB) is a substantial threat among infectious diseases, particularly considering the high risk of extrapulmonary tuberculosis (EPTB), severe forms of the disease, and the spreading of drug-resistant strains. Describing the characteristics of children with EPTB and those with drug-resistant tuberculosis (DR-TB) and analysing the role of second-line drugs could facilitate the management of these cases. This retrospective study was conducted on 271 children diagnosed with active TB disease (44 EPTB cases, 9 DR-TB cases), originating from diverse geographic areas, who were referred to the infectious disease unit at Meyer Children’s Hospital, Florence, Italy, from 2006 to 2022. In most patients, the management of therapies was complicated by the impossibility to obtain drug susceptibility testing (DST) results, which improved over the years: 17/154 (11.04%) children had DST results between 2006 and 2013, and 50/117 (42.73%, p < 0.001) between 2014 and 2022. Second-line drugs were not exclusively administered to DR-TB cases, but also to EPTB cases (20/44, 45.45%). Drugs were generally well tolerated; adverse events occurred in 13 children (13/271, 4.80%) and were generally mild and reversable. Therapies were successful in 267 children (98.52%) considered cured, while 4 (1.48%) presented sequelae. Both univariate and multivariate logistic regression analyses were conducted to investigate factors associated with EPTB, DR-TB, and second-line drugs administration. Originating from Asia emerged as a risk factor associated with both EPTB and DR-TB (p = 0.013 and p = 0.045, respectively). The introduction of GeneXpert tests has significantly improved TB diagnosis and the obtaining of DST results. The administration of second-line therapies should be limited primarily to DR-TB cases, but it is possible that these drugs may also be beneficial in selected EPTB cases.
Dynamics of Cardicola spp. Infection in Ranched Southern Bluefin Tuna: First Observation of C. orientalis at Transfer
Authors: Carabott Melissa J. , Power Cecilia , Widdicombe Maree , Rough Kirsten , Nowak Barbara F. , Bott Nathan J.
Published: December 2023

Aporocotylid blood flukes Cardicola forsteri and C. orientalis are an ongoing health concern for the Southern Bluefin Tuna (Thunnus maccoyii, SBT) industry, where infections can lead to morbidity and mortality in ranched SBT populations. This study compared blood fluke infection in SBT from two companies during the 2021 ranching season. Both companies administered the same dosage of praziquantel approximately 5 weeks after transfer, feeding with frozen baitfish daily; the only difference in the company’s practices was that the pontoons were located 2.5 km apart. Infection severity was measured as prevalence and intensity by quantifying adult C. forsteri in SBT heart and copy numbers of C. forsteri and C. orientalis ITS-2 DNA in SBT heart and gills. Data from the 2018 and 2019 harvests of SBT were used to make comparisons with 2021 harvest data. Cardicola orientalis was detected at transfer and no longer detected after treatment with praziquantel. Cardicola spp. were present in 83% of sampled SBT in 2021. Both companies demonstrated similar patterns of infection, and Company A had higher prevalence and intensity of Cardicola spp. infection. Based on C. forsteri ITS-2 DNA, infection intensity at harvest was significantly greater for both companies in 2021 when compared to 2018 and 2019. Continued monitoring of Cardicola spp. in SBT and improvements in diagnostics contribute to our understanding of Cardicola spp. epizootiology and the detection of changes in treatment efficacy.
Regulatory Effect of Ficus carica Latex on Cell Cycle Progression in Human Papillomavirus-Positive Cervical Cancer Cell Lines: Insights from Gene Expression Analysis
Authors: Cakir Muharrem , Bilge Ugur , Ghanbari Arshia , Ashrafi G.
Published: December 2023

Cervical cancer presents a significant global health concern with high-risk human papillomaviruses (HPVs) identified as the main cause of this cancer. Although current treatment methods for cervical cancer can eliminate lesions, preventing metastatic spread and minimizing tissue damage remain a major challenge. Therefore, the development of a safer and innovative therapeutic approach is of the utmost importance. Natural products like fig latex, derived from the Ficus carica tree, have demonstrated promising anti-cancer properties when tested on cervical cancer cell lines. However, the specific mechanisms by which fig latex exerts its effects are still unknown. In this study, we conducted RNA-Seq analysis to explore how fig latex may counteract carcinogenesis in HPV-positive cervical cancer cell lines, namely, CaSki (HPV type 16-positive) and HeLa (HPV type 18-positive). Our results from this investigation indicate that fig latex influences the expression of genes associated with the development and progression of cervical cancer, including pathways related to “Nonsense-Mediated Decay (NMD)”, “Cell Cycle regulation”, “Transcriptional Regulation by TP53”, and “Apoptotic Process”. This selective impact of fig latex on cancer-related pathways suggests a potential novel therapeutic approach for HPV-related cervical cancer.
Discovery of Potent Indolyl-Hydrazones as Kinase Inhibitors for Breast Cancer: Synthesis, X-ray Single-Crystal Analysis, and In Vitro and In Vivo Anti-Cancer Activity Evaluation
Authors: Salama Eid E. , Youssef Mohamed F. , Aboelmagd Ahmed , Boraei Ahmed T. A. , Nafie Mohamed S. , Haukka Matti , Barakat Assem , Sarhan Ahmed A. M.
Published: December 2023

According to data provided by the World Health Organization (WHO), a total of 2.3 million women across the globe received a diagnosis of breast cancer in the year 2020, and among these cases, 685,000 resulted in fatalities. As the incidence of breast cancer statistics continues to rise, it is imperative to explore new avenues in the ongoing battle against this disease. Therefore, a number of new indolyl-hydrazones were synthesized by reacting the ethyl 3-formyl-1H-indole-2-carboxylate 1 with thiosemicarbazide, semicarbazide.HCl, 4-nitrophenyl hydrazine, 2,4-dinitrophenyl hydrazine, and 4-amino-5-(1H-indol-2-yl)-1,2,4-triazole-3-thione to afford the new hit compounds, which were assigned chemical structures as thiosemicarbazone 3, bis(hydrazine derivative) 5, semicarbzone 6, Schiff base 8, and the corresponding hydrazones 10 and 12 by NMR, elemental analysis, and X-ray single-crystal analysis. The MTT assay was employed to investigate the compounds’ cytotoxicity against breast cancer cells (MCF-7). Cytotoxicity results disclosed potent IC50 values against MCF-7, especially compounds 5, 8, and 12, with IC50 values of 2.73 ± 0.14, 4.38 ± 0.23, and 7.03 ± 0.37 μM, respectively, compared to staurosproine (IC50 = 8.32 ± 0.43 μM). Consequently, the activities of compounds 5, 8, and 12 in relation to cell migration were investigated using the wound-healing test. The findings revealed notable wound-healing efficacy, with respective percentages of wound closure measured at 48.8%, 60.7%, and 51.8%. The impact of the hit compounds on cell proliferation was assessed by examining their apoptosis-inducing properties. Intriguingly, compound 5 exhibited a significant enhancement in cell death within MCF-7 cells, registering a notable increase of 39.26% in comparison to the untreated control group, which demonstrated only 1.27% cell death. Furthermore, the mechanism of action of compound 5 was scrutinized through testing against kinase receptors. The results revealed significant kinase inhibition, particularly against PI3K-α, PI3K-β, PI3K-δ, CDK2, AKT-1, and EGFR, showcasing promising activity, compared to standard drugs targeting these receptors. In the conclusive phase, through in vivo assay, compound 5 demonstrated a substantial reduction in tumor volume, decreasing from 106 mm³ in the untreated control to 56.4 mm³. Moreover, it significantly attenuated tumor proliferation by 46.9%. In view of these findings, the identified leads exhibit promises for potential development into future medications for the treatment of breast cancer, as they effectively hinder both cell migration and proliferation.
New Insights into the LANCL2-ABA Binding Mode towards the Evaluation of New LANCL Agonists
Authors: Scarano Naomi , Di Palma Francesco , Origlia Nicola , Musumeci Francesca , Schenone Silvia , Spinelli Sonia , Passalacqua Mario , Zocchi Elena , Sturla Laura , Cichero Elena , Cavalli Andrea
Published: December 2023

The lanthionine synthetase C-like (LANCL) proteins include LANCL2, which is expressed in the central nervous system (CNS) and in peripheral tissues. LANCL2 exhibits glutathionylation activity and is involved in the neutralization of reactive electrophiles. Several studies explored LANCL2 activation as a validated pharmacological target for diabetes and inflammatory bowel disease. In this context, LANCL2 was found to bind the natural product abscisic acid (ABA), whose pre-clinical effectiveness in different inflammatory diseases was reported in the literature. More recently, LANCL2 attracted more attention as a valuable resource in the field of neurodegenerative disorders. ABA was found to regulate neuro-inflammation and synaptic plasticity to enhance learning and memory, exhibiting promising neuroprotective effects. Up until now, a limited number of LANCL2 ligands are known; among them, BT-11 is the only compound patented and investigated for its anti-inflammatory properties. To guide the design of novel putative LANCL2 agonists, a computational study including molecular docking and long molecular dynamic (MD) simulations of both ABA and BT-11 was carried out. The results pointed out the main LANCL2 ligand chemical features towards the following virtual screening of a novel putative LANCL2 agonist (AR-42). Biochemical assays on rat H9c2 cardiomyocytes showed a similar, LANCL2-mediated stimulation by BT-11 and by AR-42 of the mitochondrial proton gradient and of the transcriptional activation of the AMPK/PGC-1α/Sirt1 axis, the master regulator of mitochondrial function, effects that are previously observed with ABA. These results may allow the development of LANCL2 agonists for the treatment of mitochondrial dysfunction, a common feature of chronic and degenerative diseases.
Synthesis, Pharmacological Properties, and Potential Molecular Mechanisms of Antitumor Activity of Betulin and Its Derivatives in Gastrointestinal Cancers
Authors: Madej Marcel , Gola Joanna , Chrobak Elwira
Published: December 2023

Gastrointestinal (GI) cancers are an increasingly common type of malignancy, caused by the unhealthy lifestyles of people worldwide. Limited methods of treatment have prompted the search for new compounds with antitumor activity, in which betulin (BE) is leading the way. BE as a compound is classified as a pentacyclic triterpene of the lupane type, having three highly reactive moieties in its structure. Its mechanism of action is based on the inhibition of key components of signaling pathways associated with proliferation, migration, interleukins, and others. BE also has a number of biological properties, i.e., anti-inflammatory, hepatoprotective, neuroprotective, as well as antitumor. Due to its poor bioavailability, betulin is subjected to chemical modifications, obtaining derivatives with proven enhanced pharmacological and pharmacokinetic properties as a result. The method of synthesis and substituents significantly influence the effect on cells and GI cancers. Moreover, the cytotoxic effect is highly dependent on the derivative as well as the individual cell line. The aim of this study is to review the methods of synthesis of BE and its derivatives, as well as its pharmacological properties and molecular mechanisms of action in colorectal cancer, hepatocellular carcinoma, gastric cancer, and esophageal cancer neoplasms.
Recent Advances in Visible Light Photoinitiating Systems Based on Flavonoids
Authors: Dumur Frédéric
Published: December 2023

The design of biosourced and/or bioinspired photoinitiators is an active research field as it offers a unique opportunity to develop photoinitiating systems exhibiting better biocompatibility as well as reduced toxicity. In this field, flavonoids can be found in numerous fruits and vegetables so these structures can be of interest for developing, in the future, polymerization processes, offering a reduced environmental impact but also better biocompatibility of the polymers. In this review, the different flavonoids reported to date as photoinitiators of polymerization are presented. Over the years, different modifications of the flavonoid scaffold have been examined including the grafting of well-known chromophores, the preparation of Type II photoinitiators or the introduction of photocleavable groups enabling the generation of Type I photoinitiators. Different families of flavonoids have also been investigated, enabling to design of high-performance photoinitiating systems.
Plant Extracellular Vesicles: Current Landscape and Future Directions
Authors: Ambrosone Alfredo , Barbulova Ani , Cappetta Elisa , Cillo Fabrizio , De Palma Monica , Ruocco Michelina , Pocsfalvi Gabriella
Published: December 2023

Plant cells secrete membrane-enclosed micrometer- and nanometer-sized vesicles that, similarly to the extracellular vesicles (EVs) released by mammalian or bacterial cells, carry a complex molecular cargo of proteins, nucleic acids, lipids, and primary and secondary metabolites. While it is technically complicated to isolate EVs from whole plants or their tissues, in vitro plant cell cultures provide excellent model systems for their study. Plant EVs have been isolated from the conditioned culture media of plant cell, pollen, hairy root, and protoplast cultures, and recent studies have gathered important structural and biological data that provide a framework to decipher their physiological roles and unveil previously unacknowledged links to their diverse biological functions. The primary function of plant EVs seems to be in the secretion that underlies cell growth and morphogenesis, cell wall composition, and cell–cell communication processes. Besides their physiological functions, plant EVs may participate in defence mechanisms against different plant pathogens, including fungi, viruses, and bacteria. Whereas edible and medicinal-plant-derived nanovesicles isolated from homogenised plant materials ex vivo are widely studied and exploited, today, plant EV research is still in its infancy. This review, for the first time, highlights the different in vitro sources that have been used to isolate plant EVs, together with the structural and biological studies that investigate the molecular cargo, and pinpoints the possible role of plant EVs as mediators in plant–pathogen interactions, which may contribute to opening up new scenarios for agricultural applications, biotechnology, and innovative strategies for plant disease management.
Institutional Context of Pest Management Science in the Global South
Authors: Wyckhuys Kris A. G. , Hadi Buyung A. R.
Published: December 2023

The natural sciences are receiving increasing attention in the Global South. This timely development may help mitigate global change and quicken an envisioned food system transformation. Yet in order to resolve complex issues such as agrochemical pollution, science ideally proceeds along suitable trajectories within appropriate institutional contexts. Here, we employ a systematic literature review to map the nature of inquiry and institutional context of pest management science in 65 low- and middle-income countries published from 2010 to 2020. Despite large inter-country variability, any given country generates an average of 5.9 publications per annum (range 0–45.9) and individual nations such as Brazil, Kenya, Benin, Vietnam, and Turkey engage extensively in regional cooperation. International development partners are prominent scientific actors in West Africa but are commonly outpaced by national institutions and foreign academia in other regions. Transnational institutions such as the CGIAR represent a 1.4-fold higher share of studies on host plant resistance but lag in public interest science disciplines such as biological control. Despite high levels of scientific abstraction, research conducted jointly with development partners shows real yet marginal improvements in incorporating the multiple (social–ecological) layers of the farming system. Added emphasis on integrative system-level approaches and agroecological or biodiversity-driven measures can extend the reach of science to unlock transformative change.
Machine Learning Analysis of the Impact of Silver Nitrate and Silver Nanoparticles on Wheat (Triticum aestivum L.): Callus Induction, Plant Regeneration, and DNA Methylation
Authors: Türkoğlu Aras , Haliloğlu Kamil , Demirel Fatih , Aydin Murat , Çiçek Semra , Yiğider Esma , Demirel Serap , Piekutowska Magdalena , Szulc Piotr , Niedbała Gniewko
Published: December 2023

The objective of this study was to comprehend the efficiency of wheat regeneration, callus induction, and DNA methylation through the application of mathematical frameworks and artificial intelligence (AI)-based models. This research aimed to explore the impact of treatments with AgNO3 and Ag-NPs on various parameters. The study specifically concentrated on analyzing RAPD profiles and modeling regeneration parameters. The treatments and molecular findings served as input variables in the modeling process. It included the use of AgNO3 and Ag-NPs at different concentrations (0, 2, 4, 6, and 8 mg L−1). The in vitro and epigenetic characteristics were analyzed using several machine learning (ML) methods, including support vector machine (SVM), random forest (RF), extreme gradient boosting (XGBoost), k-nearest neighbor classifier (KNN), and Gaussian processes classifier (GP) methods. This study’s results revealed that the highest values for callus induction (CI%) and embryogenic callus induction (EC%) occurred at a concentration of 2 mg L−1 of Ag-NPs. Additionally, the regeneration efficiency (RE) parameter reached its peak at a concentration of 8 mg L−1 of AgNO3. Taking an epigenetic approach, AgNO3 at a concentration of 2 mg L−1 demonstrated the highest levels of genomic template stability (GTS), at 79.3%. There was a positive correlation seen between increased levels of AgNO3 and DNA hypermethylation. Conversely, elevated levels of Ag-NPs were associated with DNA hypomethylation. The models were used to estimate the relationships between the input elements, including treatments, concentration, GTS rates, and Msp I and Hpa II polymorphism, and the in vitro output parameters. The findings suggested that the XGBoost model exhibited superior performance scores for callus induction (CI), as evidenced by an R2 score of 51.5%, which explained the variances. Additionally, the RF model explained 71.9% of the total variance and showed superior efficacy in terms of EC%. Furthermore, the GP model, which provided the most robust statistics for RE, yielded an R2 value of 52.5%, signifying its ability to account for a substantial portion of the total variance present in the data. This study exemplifies the application of various machine learning models in the cultivation of mature wheat embryos under the influence of treatments and concentrations involving AgNO3 and Ag-NPs.
Biochar Enhances the Resistance of Legumes and Soil Microbes to Extreme Short-Term Drought
Authors: He Kang , Liu Qiangbo , Zhang Jialei , Zhang Guanchu , Li Guolin
Published: December 2023

Short-term drought events occur more frequently and more intensively under global climate change. Biochar amendment has been documented to ameliorate the negative effects of water deficits on plant performance. Moreover, biochar can alter the soil microbial community, soil properties and soil metabolome, resulting in changes in soil functioning. We aim to reveal the extent of biochar addition on soil nutrients and the soil microbial community structure and how this improves the tolerance of legume crops (peanuts) to short-term extreme drought. We measured plant performances under different contents of biochar, set as a gradient of 2%, 3% and 4%, after an extreme experimental drought. In addition, we investigated how soil bacteria and fungi respond to biochar additions and how the soil metabolome changes in response to biochar amendments, with combined growth experiments, high-throughput sequencing and soil omics. The results indicated that biochar increased nitrites and available phosphorus. Biochar was found to influence the soil bacterial community structure more intensively than the soil fungal community. Additionally, the fungal community showed a higher randomness under biochar addition when experiencing short-term extreme drought compared to the bacterial community. Soil bacteria may be more strongly related to soil nutrient cycling in peanut agricultural systems. Although the soil metabolome has been documented to be influenced by biochar addition independent of soil moisture, we found more differential metabolites with a higher biochar content. We suggest that biochar enhances the resistance of plants and soil microbes to short-term extreme drought by indirectly modifying soil functioning probably due to direct changes in soil moisture and soil pH.
Synthesis and Characterization of Cardanol-Based Non-Isocyanate Polyurethane
Authors: Li Yanan , Zhang Bin , Zhao Yuzhuo , Lu Shuai , Fan Donglei , Wang Song , Liu Jie , Tang Tao , Li Sanxi
Published: December 2023

This paper describes the synthesis of NIPU by using cardanol as starting material. A cardanol formaldehyde oligomer was first prepared through the reaction of cardanol and formaldehyde, catalyzed by citric acid. The resulting oligomer was then subjected to epoxidation with m-chloroperbenzoic acid to obtain an epoxide compound, which was subsequently used to fix carbon dioxide (CO2) and form a cyclic carbonate. Using this cyclic carbonate, along with an amine, cardanol-based isocyanate polyurethane (NIPU) was prepared. Different characterization methods, such as Fourier transform infrared spectroscopy (FTIR), proton nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), and thermogravimetric analysis (TGA), were used to confirm the synthesis of the four intermediate products and NIPU in the reaction process. This study highlights the promise of bio-based NIPU as a sustainable alternative in a number of applications while offering insightful information on the synthesis and characterization of the material.
Effects of Different Die Metals on the Performance and Friction and Wear of Composite Materials during the Extrusion Process
Authors: Liu Hong , Wang Chuansheng
Published: December 2023

Extrusion technology is widely utilized in the rubber processing industry, with the extruder serving as the core equipment. As mixed rubber enters the extruder, it undergoes conveyance and plasticization, ultimately forming specific shapes and dimensions upon extrusion. The extruder head is a crucial component, playing a key role in achieving the final product’s required size and shape. Factors such as its structure, materials, and manufacturing processes significantly impact the efficiency, product quality, and sustainability of the extrusion process. However, prolonged operation leads to severe wear of the extruder head, adversely affecting rubber product quality. Additionally, extruder head processing poses challenges, with maintenance and repair being complex procedures. Therefore, exploring a wear-resistant, long-lasting metal material for the extruder head without compromising mixed rubber performance is essential. This study focuses on severely worn extruder head metal materials, comparing wear levels after friction with STELLITE 6 alloy, Hastelloy C-276 alloy, 38CrMoAlA, and tungsten carbide with composite rubber. Results show that compared to the NR/BR composite material after Hastelloy C-276 alloy friction, rubber Payne effect increased by 4.4% (38CrMoAl), 3.2% (STELLITE 6), and 4.6% (tungsten carbide). Similarly, rubber dispersion decreased by 9.4% (38CrMoAl), 4.7% (STELLITE 6), and 9.8% (tungsten carbide). Rolling resistance increased by 18.1% (38CrMoAl), 16% (STELLITE 6), and 23.4% (tungsten carbide). Friction coefficient increased by 3.5% (38CrMoAl), 2.8% (STELLITE 6), and 4.3% (tungsten carbide). Wear volume increased by 39.3% (38CrMoAl), 45.3% (STELLITE 6), and 48.9% (tungsten carbide). Specifically, using Hastelloy C-276 alloy as the extruder head metal material yields the best NR/BR composite material dispersion, highest ten times tear strength, excellent anti-wet skid resistance, and minimum rolling resistance. Conversely, using the other alloys results in varying reductions in the physical and mechanical properties of NR/BR composite materials. This research is crucial for improving rubber product quality and extending extruder head lifespan.
The Potential of Soluble Proteins in High-Moisture Soy Protein–Gluten Extrudates Preparation
Authors: Ning Meng , Ji Yan , Zhang Jinchuang , Pan Hongyang , Chen Jie
Published: December 2023

In this study, the effects of different soluble proteins, including collagen peptides (CP), soy protein hydrolysate (HSPI), whey protein isolate (WPI), sodium caseinate (SC), and egg white protein (EWP), on the structural and mechanical properties of blends containing soy protein isolate (SPI) and wheat gluten (WG) were investigated using high-moisture extrusion. The addition of CP and HSPI resulted in a more pronounced fibrous structure with increased voids, attributing to their plasticizing effect that enhanced polymer chain mobility and reduced viscosity. WPI, SC, and EWP acted as crosslinking agents, causing early crosslink formation and decreased polymer chain mobility. These structural variations directly influenced the tensile properties of the extrudates, with CP displaying the highest anisotropic index. Moreover, the presence of soluble proteins impacts the permeability of the extrudates. These insights shed light on how soluble proteins can be used to modify the properties of SPI-WG blends, making them suitable for meat analogue production.
Preparation and Characterization of PHBV/PCL-Diol Blend Films
Authors: Erceg Tamara , Rackov Sanja , Terek Pal , Pilić Branka
Published: December 2023

Biodegradable thin films based on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(caprolactone diol) (PCL-diol) blend were developed using the solution casting method. PHBV is biodegradable, biocompatible, and produced naturally by bacterial activity, but its use is restricted by high crystallinity and low resistance to thermal degradation with melting temperatures close to degradation thus narrowing the processing window. Solution casting was chosen as a cost-effective method reducing energy consumption and avoiding thermal degradation during processing. The increase in PCL-diol in blend composition (40–60 wt%) enhances the film-forming ability of PHBV and the wettability along with the decrease in the roughness of the resulting materials as revealed by contact angle measurements, scanning electron microscopy (SEM), and atomic force microscopy (AFM). Optimal composition in terms of filmogenity and surface structure has been achieved by the addition of PCL-diol in the amount of 60 wt%. FTIR confirmed the expected chemical structures with no evidence of chemical interactions between the two polymers.
Upcycling of HDPE Milk Bottles into High-Stiffness, High-HDT Composites with Pineapple Leaf Waste Materials
Authors: Amornsakchai Taweechai , Duangsuwan Sorn
Published: December 2023

In the pursuit of sustainability and reduced dependence on new plastic materials, this study explores the upcycling potential of high-density polyethylene (HDPE) milk bottles into high-stiffness, high-heat-distortion-temperature (HDT) composites. Recycled high-density polyethylene (rHDPE) sourced from used milk bottles serves as the composite matrix, while reinforcing fillers are derived from dried pineapple leaves, comprising fibers (PALF) and non-fibrous materials (NFM). A two-roll mixer is employed to prepare rHDPE/NFM and rHDPE/PALF mixtures, facilitating filler alignment in the resulting prepreg. The prepreg is subsequently stacked and pressed into composite sheets. The introduction of PALF as a reinforcing filler significantly enhances the flexural strength and modulus of the rHDPE composite. A 20 wt.% PALF content yields a remarkable 162% increase in flexural strength and a 204% increase in modulus compared to neat rHDPE. The rHDPE/NFM composite also shows improved mechanical properties, albeit to a lesser degree than fiber reinforcement. Both composites exhibit a slight reduction in impact resistance. Notably, the addition of NFM or PALF substantially elevates HDT, raising the HDT values of the composites to approximately 84 °C and 108 °C, respectively, in contrast to the 71 °C HDT of neat rHDPE. Furthermore, the overall properties of both the composites are further enhanced by improving their compatibility through maleic anhydride-modified polyethylene (MAPE) use. Impact fracture surfaces of both composites reveal higher compatibility and clear alignment of NFM and PALF fillers, underscoring the enhanced performance and environmental friendliness of composites produced from recycled plastics reinforced with pineapple leaf waste fillers.
Economical Design of Drip Irrigation Control System Management Based on the Chaos Beetle Search Algorithm
Authors: Zhang Yue , Song Chenchen
Published: December 2023

In the realm of existing intelligent drip irrigation control systems, traditional PID control encounters challenges in delivering satisfactory control outcomes, primarily owing to issues related to non-linearity, time-varying behavior, and hysteresis. In order to solve the problem of the unstable operation of the drip irrigation system in an intelligent irrigation system, this paper proposes chaotic beetle swarm optimization (CBSO) based on the BAS (beetle antennae search) longicorn search algorithm, with inertial weights, variable learning factors, and logistic chaos initialization improving global search capabilities. This was accomplished by formulating the optimization objective, which involved integrating the control input’s time integral term, the square term, and the absolute value of the error. Subsequently, PID parameter tuning was performed. In order to verify the actual effect of the CBSO algorithm on the PID drip irrigation control system, MATLAB was used to simulate and compare PID control optimized by the GA algorithm, PSO algorithm, and BSO (beetle search optimization) algorithm. The results show that PID control based on CBSO optimization has a short response time, small overshoot, and no oscillation in the steady state process. The performance of the controller is improved, which provides a basis for PID parameter setting for a drip irrigation control system.
A Study on the Production Simulation of Coal–Shale Interbedded Coal Measure Superimposed Gas Reservoirs under Different Drainage Methods
Authors: Wang Wenkai , Liu Shiqi , Sang Shuxun , Du Ruibin , Liu Yinghai
Published: December 2023

To study the influence of different drainage methods on the production performance of coal measure gas wells, the interbedded reservoir composed of coal and shale in the Longtan Formation of the Dahebian block was used as the research object. Considering the influence of coal and shale matrix shrinkage, effective stress, and interlayer fluid flow on reservoir properties such as fluid migration behavior and permeability, a fluid–solid coupling mathematical model of coal measure superimposed gas reservoirs was established. Numerical simulations of coal measure gas production under different drainage and production modes were conducted to analyze the evolution of reservoir pressure, gas content in the matrix, permeability, and other characteristic parameters of the superimposed reservoir, as well as differences in interlayer flow. The results showed that, compared to single-layer drainage, cumulative gas production increased by 33% under multi-layer drainage. Both drainage methods involve interlayer energy and substance transfer. Due to the influence of permeability, porosity, and mechanical properties, significant differences exist in reservoir pressure distribution, preferential flow direction, gas content in the matrix, and permeability ratio between coal and shale reservoirs under different drainage and production modes. Multi-layer drainage effectively alleviates the influence of vertical reservoir pressure differences between reservoir layers, facilitates reservoir pressure transmission in shale reservoirs, enhances methane desorption in shale matrices, promotes matrix shrinkage, and induces the rebound of shale reservoir permeability, thus improving overall gas production.
Financial Stress and COVID-19: A Comprehensive Analysis of the Factors Associated with the Pandemic
Authors: Moon Keewon , Heo Wookjae , Lee Jae Min , Grable John E.
Published: December 2023

The COVID-19 pandemic introduced unprecedented challenges for households globally, serving as a precursor to and trigger for financial stress. This study examined the associations across various factors thought to be associated with financial stress (a psychological syndrome) resulting from the COVID-19 pandemic. Using survey data collected in 2019 (n = 997) and 2021 (n = 988), propensity score matching and hierarchical linear modeling were employed to identify the association between financial stress and the pandemic. Results indicated that financial stress increased during the COVID-19 pandemic. Three covariate groups, including financial characteristics, health status, and socio-demographic characteristics, were found to be associated with financial stress levels. The primary contribution of this paper lies in offering a comprehensive understanding of how the dynamics of financial stress evolve with shifting macroeconomic events. This paper serves as a framework to employ a comprehensive financial stress measure and matched samples at various data points. Findings from this study contribute to the existing literature on financial well-being, financial stress, and societal outcomes associated with global health events while providing implications for policy and practice.
Investigation of the Source of Iceland Basin Freshening: Virtual Particle Tracking with Satellite-Derived Geostrophic Surface Velocities
Authors: Furey Heather H. , Foukal Nicholas P. , Anderson Adele , Bower Amy S.
Published: December 2023

In the 2010s, a large freshening event similar to past Great Salinity Anomalies occurred in the Iceland Basin that has since propagated into the Irminger Sea. The source waters of this fresh anomaly were hypothesized to have come from an eastward diversion of the Labrador Current, a finding that has since been supported by recent modeling studies. In this study, we investigate the pathways of the freshwater anomaly using a purely observational approach: particle tracking using satellite altimetry-derived surface velocity fields. Particle trajectories originating in the Labrador Current and integrated forward in time entered the Iceland Basin during the freshening event at nearly twice the frequency observed prior to 2009, suggesting an increased presence of Labrador Current-origin water in the Iceland Basin and Rockall Trough during the freshening. We observe a distinct regime change in 2009, similar to the timing found in the previous modeling papers. These spatial shifts were accompanied by faster transit times along the pathways which led to along-stream convergence and more particles arriving to the eastern subpolar gyre. These findings support the hypothesis that a diversion of relatively fresh Labrador Current waters eastward from the Grand Banks can explain the unprecedented freshening in the Iceland Basin.
A Low Cost Real-Time Transient Recorder for High Voltage Systems
Authors: Grebović Selma , Oprašić Nermin , Fejzić Ajdin , Kartal Harun , Akšamović Abdulah , Konjicija Samim
Published: December 2023

Large-scale incorporation of new energy generation units based on renewable sources, such as wind and photovoltaic power, drastically alters the structure of the power system. Because of the intermittent nature of these sources, switching in grids (connection and disconnection) occurs much more frequently than with conventional sources. As a result, the power system will inevitably experience a large number of transients, which raises questions about the stability of the system and the quality of the electrical energy. Therefore, measuring various types of transients in power system is crucial for stability, power quality, fault analysis, protection design, and insulation design. Transient recorders that are currently used are generally expensive and only suitable for particular locations in power systems. The number of installed transient recorders is insufficient for a comprehensive analysis of problems that may occur. Hence, it is important to have inexpensive and efficient transient recorders that can be installed at multiple points in the power system on various types of objects. It is also essential to have a transient record database with open access, which can be used by researchers to develop new analysis techniques based on artificial intelligence. This paper proposes an inexpensive measurement and acquisition system designed to record transient phenomena on different objects within the power system. The system is designed to use autonomous power, a standardized data acquisition module, a low-budget system for transmitting recorded transient events to the server via mobile network, and a sensor system adapted to the object where transients are recorded. The proposed system is designed to be used for all types of objects in the power system where transients may occur, such as power lines, transmission towers, surge arresters, and transformers. All components of the system are described, and the system is tested under laboratory conditions. The modular nature of the system allows customization to the specifics of the location in power system by choosing appropriate components. The calibration method of the custom designed Rogowski coil is described. The cost analysis of the proposed system and power consumption analysis are performed. The results show that the system’s performance meets application requirements at a low cost.
Enhancing Safety on Construction Sites: A UWB-Based Proximity Warning System Ensuring GDPR Compliance to Prevent Collision Hazards
Authors: Mastrolembo Ventura Silvia , Bellagente Paolo , Rinaldi Stefano , Flammini Alessandra , Ciribini Angelo L. C.
Published: December 2023

Construction is known as one of the most dangerous industries in terms of worker safety. Collisions due the excessive proximity of workers to moving construction vehicles are one of the leading causes of fatal and non-fatal accidents on construction sites internationally. Proximity warning systems (PWS) have been proposed in the literature as a solution to detect the risk for collision and to alert workers and equipment operators in time to prevent collisions. Although the role of sensing technologies for situational awareness has been recognised in previous studies, several factors still need to be considered. This paper describes the design of a prototype sensor-based PWS, aimed mainly at small and medium-sized construction companies, to collect real-time data directly from construction sites and to warn workers of a potential risk of collision accidents. It considers, in an integrated manner, factors such as cost of deployment, the actual nature of a construction site as an operating environment and data protection. A low-cost, ultra-wideband (UWB)-based proximity detection system has been developed that can operate with or without fixed anchors. In addition, the PWS is compliant with the General Data Protection Regulation (GDPR) of the European Union. A privacy-by-design approach has been adopted and privacy mechanisms have been used for data protection. Future work could evaluate the PWS in real operational conditions and incorporate additional factors for its further development, such as studies on the timely interpretation of data.
Electromagnetic Detection System with Magnetic Dipole Source for Near-Surface Detection
Authors: Liao Xian , Xu Zhengyu , Liu Wei , Tai Heng-Ming , Zhou Jie , Ma Xiao , Fu Zhihong
Published: December 2023

This paper proposes a nondestructive, separate transmitter-receiver (TX-RX) electromagnetic measurement system for near-surface detection. Different from the traditional dual-coil integrated design, the proposed transient electromagnetic (TEM) system performs shallow subsurface detection using independent TX coil and movable RX coils. This configuration requires a large primary field so that the far-away secondary field is able to generate reliably induced voltages. To achieve this goal, a bipolar current-pulsed power supply (BCPPS) with a late resonant charging strategy is designed to produce a sufficiently large magnetic moment for the exciting coil with low source interference. The magnetic dipole source (MDS) with a large proportion of weight is separated from the field observation device and does not need to be dragged or transported during the detection process. This setup lowers the weight of the scanning device to 3 kg and greatly improves the measurement efficiency. The results of the laboratory test verify the effectiveness of the separate MDS and RX module system. Field experimental detection further demonstrates that the proposed system can realize highly efficient and shallow surface detection within a 200 m range of the MDS device.