bluuebunny/crossref_metadata_2025 huggingface dataset:
Determination of Seed Volume Based on Selected Seed Dimensions
Authors: Kaliniewicz Zdzisław , Choszcz Dariusz , Lipiński Adam
Published: September 2022

The volume coefficient, which denotes a simple relationship between selected seed dimensions and seed volume, can be used to facilitate volume calculations in individual seeds, in particular in species with a complex seed shape. For this reason, seed thickness, width, and length were measured in nine species of forest trees and shrubs. The volume of seeds belonging to each plant species was determined by pycnometry, and the results were used to calculate 10 volume coefficients based on different combinations of basic seed dimensions. The calculated coefficients had different values, and they were lowest when volume was determined based on the cube of seed length and highest when volume was determined based on the cube of seed thickness. In a formula based on all three basic dimensions, the calculated volume coefficient ranged from 0.376 to 0.537, and Cornus macrophylla, Picea abies, and Cornus sanguinea seeds most closely resembled an ellipsoid. When seed volume was determined with the use of two basic dimensions, formulas based on the square of the smaller dimension produced somewhat smaller errors in individual seeds. In turn, seed thickness should be used in formulas that rely on a single dimension. Seed volume coefficients were most strongly correlated with the sphericity index, which indicates that this parameter can be used to estimate their values. The sphericity index was most strongly correlated with volume coefficients; the strongest correlations were observed for volume coefficients calculated based on the square of the seed length and seed width, and the cube of the seed length.
A Quasi-Quadratic Inverse Scattering Approach to Detect and Localize Metallic Bars within a Dielectric
Authors: Brancaccio Adriana
Published: September 2022

The inverse scattering problem related to the localization of metal bars embedded within a finite-dimensional dielectric was studied in two-dimensional geometry. The dielectric was placed in air and illuminated from the outside using a linear microwave source and a multi-monostatic configuration. The discontinuity at the interface between the air and the dielectric causes reflections that are neglected if a simple linear Born approximation of scattering is assumed. Herein, a new formulation was proposed based on a quadratic approximation of the scattering equation. The formulation maintained the interaction between the metal bars and the dielectric edge, whereas the mutual coupling between the bars was neglected. By exploiting the knowledge of the permittivity of the dielectric and the shape of its section, a relatively simple approximate expression for the scattered field was derived, which allowed for formulation of an inverse linear problem. Numerical examples demonstrated the feasibility of this approach.
High Speed Decoding for High-Rate and Short-Length Reed–Muller Code Using Auto-Decoder
Authors: Cho Hyun Woo , Song Young Joon
Published: September 2022

In this paper, we show that applying a machine learning technique called auto-decoder (AD) to high-rate and short length Reed–Muller (RM) decoding enables it to achieve maximum likelihood decoding (MLD) performance and faster decoding speed than when fast Hadamard transform (FHT) is applied in additive white Gaussian noise (AWGN) channels. The decoding speed is approximately 1.8 times and 125 times faster than the FHT decoding for R(1,4) and R(2,4), respectively. The number of nodes in the hidden layer of AD is larger than that of the input layer, unlike the conventional auto-encoder (AE). Two ADs are combined in parallel and merged together, and then cascaded to one fully connected layer to improve the bit error rate (BER) performance of the code.
A Phase Correlation Anti-Noise Displacement Estimation Method Based on PSD Phase Screening
Authors: Zhang Yunsheng , Yang Zhen , Xing Lei , Chen Siyang , Dai Wujiao , Chai Jiaxin
Published: September 2022

When using digital images to obtain the displacement of a structure, noise is always a factor that greatly affects the accuracy of the displacement results. To overcome this problem, a phase-correlation improvement method, based on phase filtering by phase standard deviation (PSD), is proposed. We use PSD to remove the severe noise interference area in the cross-power spectrum matrix, then the singular value decomposition (SVD) is used to convert the two-dimensional phase to one-dimensional to reduce the difficulty of phase unwrapping. Finally, straight-line fitting is used to obtain the high-precision displacement results. Experiments were carried out using both simulated data and real data. For the simulated data, the absolute accuracy of the proposed method is around 0.3 pixels, while, for the real data, the absolute accuracy of the proposed method is 0.018 mm. The experimental results show that the method can effectively resist noise interference and still obtain accurate displacement estimation results in the case of severe noise.
Pyramid Spatial-Temporal Graph Transformer for Skeleton-Based Action Recognition
Authors: Chen Shuo , Xu Ke , Jiang Xinghao , Sun Tanfeng
Published: September 2022

Although graph convolutional networks (GCNs) have shown their demonstrated ability in skeleton-based action recognition, both the spatial and the temporal connections rely too much on the predefined skeleton graph, which imposes a fixed prior knowledge for the aggregation of high-level semantic information via the graph-based convolution. Some previous GCN-based works introduced dynamic topology (vertex connection relationships) to capture flexible spatial correlations from different actions. Then, the local relationships from both the spatial and temporal domains can be captured by diverse GCNs. This paper introduces a more straightforward and more effective backbone to obtain the spatial-temporal correlation between skeleton joints with a local-global alternation pyramid architecture for skeleton-based action recognition, namely the pyramid spatial-temporal graph transformer (PGT). The PGT consists of four stages with similar architecture but different scales: graph embedding and transformer blocks. We introduce two kinds of transformer blocks in our work: the spatial-temporal transformer block and joint transformer block. In the former, spatial-temporal separated attention (STSA) is proposed to calculate the connection of the global nodes of the graph. Due to the spatial-temporal transformer block, self-attention can be performed on skeleton graphs with long-range temporal and large-scale spatial aggregation. The joint transformer block flattens the tokens in both the spatial and temporal domains to jointly capture the overall spatial-temporal correlations. The PGT is evaluated on three public skeleton datasets: the NTU RGBD 60, NTU RGBD 120 and NW-UCLA datasets. Better or comparable performance with the state of the art (SOTA) shows the effectiveness of our work.
Prediction of Urban Thermal Environment Based on Multi-Dimensional Nature and Urban Form Factors
Authors: Wang Yueyao , Liang Ze , Ding Jiaqi , Shen Jiashu , Wei Feili , Li Shuangcheng
Published: September 2022

The urban thermal environment is affected by multiple urban form and natural environment factors; research on the accurate prediction of the urban thermal environment, considering the interaction among different urban environmental factors, is still lacking. The development of a machine learning model provides a good means of solving complex problems. This study aims to clarify the relationship between urban environmental variables and the urban thermal environment through high-precision machine learning models as well as provide scenarios of future urban thermal environment developments. We defined an urban thermal environment index (UTEI), considering twelve urban form and natural indicators sourced from the remote sensing data of 150 cities in the Jing-Jin-Ji region from 2000 to 2015. We achieved accurate predictions of UTEI through training a gradient-boosted regression trees model. By unpacking the model, we found that the contribution rate of elevation (ELEV) was the highest. Among all the urban form indicators, the elongation index (ELONG), urban population (POP), nighttime light intensity (NLI), urban area size (AREA), and urban shape index (SHAPE) also had high contributions. We set up five scenarios to simulate the possible impact of different urban form factors on the overall urban thermal environment quality in the region. Under extremely deteriorated patterns that do not control urban expansion and vegetation reduction, the average UTEI could be as high as 0.55–0.76 °C in summer and 0.24–0.29 °C in winter, yet in the extremely optimized situation, UTEI decreased by 0.69 °C in summer and 0.56 °C in winter. Results showed that better urban form improves the quality of urban environments and can provide important insights for urban planners to mitigate urban heat island problems.
Cutting Cement Industry CO2 Emissions through Metakaolin Use in Construction
Authors: Abiodun Yetunde Oyebolaji , Olanrewaju Oludolapo Akanni , Gbenebor Oluwashina Philips , Ochulor Ezenwanyi Fidelia , Obasa Dumebi Victoria , Adeosun Samson Oluropo
Published: September 2022

Cement production is one of the most important industries on the planet, and humans have relied on is use dating back to the dawn of civilization. Cement manufacturing has increased at an exponential rate, reaching 3 billion metric tons in 2015, representing a 6.3% annual growth rate and accounting for around 5–8% of global carbon dioxide (CO2) emissions. Geopolymer materials, which are inorganic polymers made from a wide range of aluminosilicate powders, such as metakaolin, fly ash, and blast furnace or steel slags, have also been elicited for use due to concerns about the high energy consumption and CO2 emissions connected with cement and concrete manufacturing. This study focused on the mechanical and durability properties of metakaolin in concrete production. X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive spectroscopy (EDS) analyses were used to confirm the characteristics of kaolin and metakaolin. The results showed that 15 wt.% metakaolin can be used to partially replace cement, and that metakaolin, when synthesized with alkaline activators, can also be utilized as a geopolymer to totally replace cement in concrete production. For predicting the compressive strength of different concrete mixtures, few practical models have been presented. This research has shed light on the possibility of utilizing ecologically friendly materials in the building, construction, and transportation sectors to decrease carbon dioxide emissions.
Developing the New Thermal Climate Zones of China for Building Energy Efficiency Using the Cluster Approach
Authors: Bai Lujian , Song Bing , Yang Liu
Published: September 2022

Climate is a key element in building design. The determination of adequate climate zoning is crucial for achieving building energy efficiency and reduced carbon emissions. In this study, a cluster analysis method was applied to develop new thermal climate zones for building envelope thermal design in China. In total, three different cluster analysis methods, including k-means, average-linkage, and Ward’s clustering, were considered. The analysis indicated that the average-linkage clustering was more appropriate for this study, and the elbow method could not accurately determine the best number of categories of average-linkage clustering. Further analysis showed that the unsupervised cluster processes may generate an unavoidable redundancy category and, to obtain precise results, supervision may be necessary in some contexts. Finally, China was classified into 10 climate zones. The North China plain and Qinghai–Tibet plateau are classified into two independent climate zones, and Turpan and Bayanbulak were classified into two newly defined climate zones different from their surrounding area in the new definition. Quantitative analysis indicated that the new zones were more precise when compared to the current ones, which can provide more precise climate information and contribute to formulating more precise standards and policies related to the thermal design of building envelopes in the future.
Controllability Results for First Order Impulsive Fuzzy Differential Systems
Authors: Liu Rui , Fečkan Michal , O’Regan Donal , Wang Jinrong
Published: September 2022

In this paper, we investigate the controllability of first-order impulsive fuzzy differential equations. Using the direct construction method, the controllability of first-order linear impulsive fuzzy differential equations is considered with a<0, the (c1) solution, and a<0, the (c2) solution, respectively.In addition, by employing the Banach fixed-point theorem, the controllability of first-order nonlinear impulsive fuzzy differential equations is studied. Finally, examples are presented to illustrate our theoretical results.
Virtual Scoliosis Surgery Using a 3D-Printed Model Based on Biplanar Radiographs
Authors: Courvoisier Aurélien , Cebrian Antonio , Simon Julien , Désauté Pascal , Aubert Benjamin , Amabile Célia , Thiébaut Lucie
Published: September 2022

The aim of this paper is to describe a protocol that simulates the spinal surgery undergone by adolescents with idiopathic scoliosis (AIS) by using a 3D-printed spine model. Patients with AIS underwent pre- and postoperative bi-planar low-dose X-rays from which a numerical 3D model of their spine was generated. The preoperative numerical spine model was subsequently 3D printed to virtually reproduce the spine surgery. Special consideration was given to the printing materials for the 3D-printed elements in order to reflect the radiopaque and mechanical properties of typical bones most accurately. Two patients with AIS were recruited and operated. During the virtual surgery, both pre- and postoperative images of the 3D-printed spine model were acquired. The proposed 3D-printing workflow used to create a realistic 3D-printed spine suitable for virtual surgery appears to be feasible and reliable. This method could be used for virtual-reality scoliosis surgery training incorporating 3D-printed models, and to test surgical instruments and implants.
Normothermic Ex Vivo Liver Platform Using Porcine Slaughterhouse Livers for Disease Modeling
Authors: Krüger Melanie , Ruppelt Alicia , Kappler Benjamin , Van Soest Elke , Samsom Roos Anne , Grinwis Guy C. M. , Geijsen Niels , Helms J. Bernd , Stijnen Marco , Kock Linda M. , Rasponi Marco , Kooistra Hans S. , Spee Bart
Published: September 2022

Metabolic and toxic liver disorders, such as fatty liver disease (steatosis) and drug-induced liver injury, are highly prevalent and potentially life-threatening. To allow for the study of these disorders from the early stages onward, without using experimental animals, we collected porcine livers in a slaughterhouse and perfused these livers normothermically. With our simplified protocol, the perfused slaughterhouse livers remained viable and functional over five hours of perfusion, as shown by hemodynamics, bile production, indocyanine green clearance, ammonia metabolism, gene expression and histology. As a proof-of-concept to study liver disorders, we show that an infusion of free fatty acids and acetaminophen results in early biochemical signs of liver damage, including reduced functionality. In conclusion, the present platform offers an accessible system to perform research in a functional, relevant large animal model while avoiding using experimental animals. With further improvements to the model, prolonged exposure could make this model a versatile tool for studying liver diseases and potential treatments.
Fucoidan from Marine Macroalgae: Biological Actions and Applications in Regenerative Medicine, Drug Delivery Systems and Food Industry
Authors: Anisha Grace Sathyanesan , Padmakumari Savitha , Patel Anil Kumar , Pandey Ashok , Singhania Reeta Rani
Published: September 2022

The marine macroalgae produce a collection of bioactive polysaccharides, of which the sulfated heteropolysaccharide fucoidan produced by brown algae of the class Phaeophyceae has received worldwide attention because of its particular biological actions that confer nutritional and health benefits to humans and animals. The biological actions of fucoidan are determined by their structure and chemical composition, which are largely influenced by the geographical location, harvest season, extraction process, etc. This review discusses the structure, chemical composition and physicochemical properties of fucoidan. The biological action of fucoidan and its applications for human health, tissue engineering, regenerative medicine and drug delivery are also addressed. The industrial scenario and prospects of research depicted would give an insight into developing fucoidan as a commercially viable and sustainable bioactive material in the nutritional and pharmacological sectors.
Dental Paleobiology in a Juvenile Neanderthal (Combe-Grenal, Southwestern France)
Authors: Garralda María Dolores , Weiner Steve , Arensburg Baruch , Maureille Bruno , Vandermeersch Bernard
Published: September 2022

Combe-Grenal site (Southwest France) was excavated by F. Bordes between 1953 and 1965. He found several human remains in Mousterian levels 60, 39, 35 and especially 25, corresponding to MIS 4 (~75–70/60 ky BP) and with Quina Mousterian lithics. One of the fossils found in level 25 is Combe-Grenal IV, consisting of a fragment of the left corpus of a juvenile mandible. This fragment displays initial juvenile periodontitis, and the two preserved teeth (LLP4 and LLM1) show moderate attrition and dental calculus. The SEM tartar analysis demonstrates the presence of cocci and filamentous types of bacteria, the former being more prevalent. This result is quite different from those obtained for the two adult Neanderthals Kebara 2 and Subalyuk 1, where more filamentous bacteria appear, especially in the Subalyuk 1 sample from Central Europe. These findings agree with the available biomedical data on periodontitis and tartar development in extant individuals, despite the different environmental conditions and diets documented by numerous archeological, taphonomical and geological data available on Neanderthals and present-day populations. New metagenomic analyses are extending this information, and despite the inherent difficulties, they will open important perspectives in studying this ancient human pathology.
Conservation Biology and Reproduction in a Time of Developmental Plasticity
Authors: Holt William V. , Comizzoli Pierre
Published: September 2022

The objective of this review is to ask whether, and how, principles in conservation biology may need to be revisited in light of new knowledge about the power of epigenetics to alter developmental pathways. Importantly, conservation breeding programmes, used widely by zoological parks and aquariums, may appear in some cases to reduce fitness by decreasing animals’ abilities to cope when confronted with the ‘wild side’ of their natural habitats. Would less comfortable captive conditions lead to the selection of individuals that, despite being adapted to life in a captive environment, be better able to thrive if relocated to a more natural environment? While threatened populations may benefit from advanced reproductive technologies, these may actually induce undesirable epigenetic changes. Thus, there may be inherent risks to the health and welfare of offspring (as is suspected in humans). Advanced breeding technologies, especially those that aim to regenerate the rarest species using stem cell reprogramming and artificial gametes, may also lead to unwanted epigenetic modifications. Current knowledge is still incomplete, and therefore ethical decisions about novel breeding methods remain controversial and difficult to resolve.
Progesterone as an Anti-Inflammatory Drug and Immunomodulator: New Aspects in Hormonal Regulation of the Inflammation
Authors: Fedotcheva Tatiana A. , Fedotcheva Nadezhda I. , Shimanovsky Nikolai L.
Published: September 2022

The specific regulation of inflammatory processes by steroid hormones has been actively studied in recent years, especially by progesterone (P4) and progestins. The mechanisms of the anti-inflammatory and immunomodulatory P4 action are not fully clear. The anti-inflammatory effects of P4 can be defined as nonspecific, associated with the inhibition of NF-κB and COX, as well as the inhibition of prostaglandin synthesis, or as specific, associated with the regulation of T-cell activation, the regulation of the production of pro- and anti-inflammatory cytokines, and the phenomenon of immune tolerance. The specific anti-inflammatory effects of P4 and its derivatives (progestins) can also include the inhibition of proliferative signaling pathways and the antagonistic action against estrogen receptor beta-mediated signaling as a proinflammatory and mitogenic factor. The anti-inflammatory action of P4 is accomplished through the participation of progesterone receptor (PR) chaperones HSP90, as well as immunophilins FKBP51 and FKBP52, which are the validated targets of clinically approved immunosuppressive drugs. The immunomodulatory and anti-inflammatory effects of HSP90 inhibitors, tacrolimus and cyclosporine, are manifested, among other factors, due to their participation in the formation of an active ligand–receptor complex of P4 and their interaction with its constituent immunophilins. Pharmacological agents such as HSP90 inhibitors can restore the lost anti-inflammatory effect of glucocorticoids and P4 in chronic inflammatory and autoimmune diseases. By regulating the activity of FKBP51 and FKBP52, it is possible to increase or decrease hormonal signaling, as well as restore it during the development of hormone resistance. The combined action of immunophilin suppressors with steroid hormones may be a promising strategy in the treatment of chronic inflammatory and autoimmune diseases, including endometriosis, stress-related disorders, rheumatoid arthritis, and miscarriages. Presumably, the hormone receptor- and immunophilin-targeted drugs may act synergistically, allowing for a lower dose of each.
Molecular Imaging and Quantification of Smooth Muscle Cell and Aortic Tissue Calcification In Vitro and Ex Vivo with a Fluorescent Hydroxyapatite-Specific Probe
Authors: Greco Anna , Herrmann Jaqueline , Babic Milen , Gummi Manasa Reddy , van der Giet Markus , Tölle Markus , Schuchardt Mirjam
Published: September 2022

Vessel calcification is characterized by the precipitation of hydroxyapatite (HAP) in the vasculature. Currently, no causal therapy exists to reduce or prevent vessel calcification. Studying the underlying pathways within vascular smooth muscle cells and testing pharmacological intervention is a major challenge in the vascular research field. This study aims to establish a rapid and efficient working protocol for specific HAP detection in cells and tissue using the synthetic bisphosphonate fluorescence dye OsteoSense™. This protocol facilitates especially early quantification of the fluorescence signal and permits co-staining with other markers of interest, enabling smaller experimental set-ups with lesser primary cells consumption and fast workflows. The fluorescence-based detection of vascular calcification with OsteoSense™ combines a high specificity with improved sensitivity. Therefore, this methodology can improve research of the pathogenesis of vascular calcification, especially for testing the therapeutic benefit of inhibitors in the case of in vitro and ex vivo settings.
Fertility Preservation and Reproductive Potential in Transgender and Gender Fluid Population
Authors: Choi Ji Young , Kim Tae Jin
Published: September 2022

The gender diverse and transgender community is a minor patient group that is encountered with increasing frequency in the clinical setting, attributed to the improved awareness and access to medical facilities. Partial impairment to permanent elimination of fertility potential and outcomes depending on the treatment modality usually is a result of gender-affirming therapy, which includes both hormone therapy and surgical intervention. Although seldom conducted in the clinical field, transgender patients should be counseled on their fertility preservation options prior to medical and surgical gender transition. There is relatively limited data and clinical information regarding fertility preservation for transgender individuals. Current treatment regimens are based on protocols from fertility preservation after oncological treatments. Major barriers for the transgender population exist due to the lack of information provided and clinical narrative that is not familiar to the physician or health care provider, although there are various options for fertility preservation. A deeper understanding of this clinical agenda and the mandatory processes will ultimately result in a much more comprehensive and specific care for transgender individuals who are in great need for fertility counseling or treatment options that concern fertility preservation. In this review, current clinical approaches will be summarized and fertility preservation options along with ongoing and future clinical trials in fertility preservation for transgender individuals will be thoroughly reviewed.
Analysis of Risk Factors for Phonation Disorders after Thyroid Surgery
Authors: Głód Mateusz , Marciniak Dominik , Kaliszewski Krzysztof , Sutkowski Krzysztof , Rudnicki Jerzy , Bolanowski Marek , Wojtczak Beata
Published: September 2022

Phonation disorders after thyroidectomy are among the most common complications and occur as a result of recurrent laryngeal nerve (RLN) injury. The multivariate analysis of risk factors for phonation disorders after thyroidectomy was assessed. A group of 830 patients with 1500 RLNs at risk of injury during thyroidectomy were analyzed retrospectively. The impact of the method of RLN identification, age, sex, BMI, kind of thyroid surgery, pathology, surgeon’s experience and thyroid volume on vocal cord paralysis was analyzed. We found that the retrosternal goiter and the volume above 100 mL were the most important risk factors for both transient and permanent paralysis. Thyroid cancer had a statistically significant impact on the increase in permanent paralysis, while this indication had practically no impact on transient paralysis. Among patients over 65 years with obesity, the probability of transient complications approximately doubled, with no effect on the permanent paralysis. Men were approximately 1.7 times more likely to develop any type of phonation disorder. Secondary operations more than doubled the risk of transient and permanent vocal cord paralysis. Thyroidectomy with only visual RLN identification was associated with a risk of both transient and permanent vocal cord paralysis almost two times higher, compared to neuromonitoring.
Association of Hypernatremia with Immune Profiles and Clinical Outcomes in Adult Intensive Care Unit Patients with Sepsis
Authors: Lin Chiung-Yu , Chen Yu-Mu , Tsai Yi-Hsuan , Hung Kai-Yin , Fang Ying-Tang , Chang Yu-Ping , Tsai Meng-Yun , Wu Hsuan-Feng , Lin Meng-Chih , Fang Wen-Feng
Published: September 2022

Both hypernatremia and an abnormal immune response may increase hospital mortality in patients with sepsis. This study examined the association of hypernatremia with abnormal immune response and mortality in 520 adult patients with sepsis in an intensive care unit (ICU). We compared the mortality and ex vivo lipopolysaccharide (LPS)-induced inflammatory response differences among patients with hyponatremia, eunatremia, and hypernatremia, as well as between patients with acquired hypernatremia on ICU day 3 and those with sustained eunatremia over first three ICU days. Compared with eunatremia or hyponatremia, hypernatremia led to higher 7 day, 14 day, 28 day, and hospital mortality rates (p = 0.030, 0.009, 0.010, and 0.033, respectively). Compared with sustained eunatremia, acquired hypernatremia led to higher 7, 14, and 28 day mortality rates (p = 0.019, 0.042, and 0.028, respectively). The acquired hypernatremia group nonsignificantly trended toward increased hospital mortality (p = 0.056). Day 1 granulocyte colony-stimulating factor (G-CSF) and tumor necrosis factor (TNF) α levels were relatively low in patients with hypernatremia (p = 0.020 and 0.010, respectively) but relatively high in patients with acquired hypernatremia (p = 0.049 and 0.009, respectively). Thus, in ICU-admitted septic patients, hypernatremia on admission and in ICU-acquired hypernatremia were both associated with higher mortality. The higher mortality in patients with hypernatremia on admission was possibly related to the downregulation of G-CSF and TNF-α secretion after endotoxin stimulation. Compared to sustained eunatremia, acquired hypernatremia showed immunoparalysis at first and then hyperinflammation on day 3.
Effects of Magnesium Oxide Nanoparticles Incorporation on Shear Bond Strength and Antibacterial Activity of an Orthodontic Composite: An In Vitro Study
Authors: Rangrazi Abdolrasoul , Daneshmand Maryam Sadat , Ghazvini Kiarash , Shafaee Hooman
Published: September 2022

This study aimed to evaluate the effects of magnesium oxide (MgO) nanoparticle (NP) incorporation on shear bond strength (SBS) and antibacterial property of orthodontic composites. A total of 100 mounted premolar teeth were randomly divided into five groups. In group 1 (control), the brackets were bonded to the teeth using the GC Ortho Connect orthodontic composite, while the brackets of groups 2 to 5 were bonded by the GC Ortho Connect orthodontic composite that contained 0.5%, 1%, 2%, and 4% weight percentages (w/w) of MgO NPs, respectively and then the SBS was measured. In the following, we evaluated the antibacterial properties of the MgO NP-containing composite on Streptococcus mutans (S. mutans) bacteria by the direct contact test method. According to results, there were no significant changes in the SBS as the MgO NP concentration was increased up to 1%, while the SBSs of the 2% and 4% MgO NPs were decreased when compared to the other three groups. The outcomes of the direct contact test indicated the case of 1% as being the minimum ratio of MgO NPs, which almost caused the entire annihilation of the S. mutans bacteria. In conclusion, the orthodontic composite containing 1% MgO NPs can display a significant antibacterial effect against S. mutans bacteria without inducing any negative effect on the SBS.
The Utilization of Tunable Transducer Elements Formed by the Manipulation of Magnetic Beads with Different Sizes via Optically Induced Dielectrophoresis (ODEP) for High Signal-to-Noise Ratios (SNRs) and Multiplex Fluorescence-Based Biosensing Applications
Authors: Yang Chia-Ming , Yu Jian-Cyun , Chu Po-Yu , Hsieh Chia-Hsun , Wu Min-Hsien
Published: September 2022

Magnetic beads improve biosensing performance by means of their small volume and controllability by magnetic force. In this study, a new technique composed of optically induced dielectrodphoresis (ODEP) manipulation and image processing was used to enhance the signal-to-noise ratio of the fluorescence for stained magnetic beads. According to natural advantages of size-dependent particle isolation by ODEP manipulation, biomarkers in clinical samples can be easily separated by different sizes of magnetic beads with corresponding captured antibodies, and rapidly distinguished by separated location of immunofluorescence. To verify the feasibility of the concept, magnetic beads with three different diameters, including 21.8, 8.7, and 4.2 μm, were easily separated and collected into specific patterns in the defined target zone treated as three dynamic transducer elements to evaluate fluorescence results. In magnetic beads with diameter of 4.2 μm, the lowest signal-to-noise ratio between stained and nonstained magnetic beads was 3.5. With the help of ODEP accumulation and detection threshold setting of 32, the signal-to-noise ratio was increased to 77.4, which makes this method more reliable. With the further optimization of specific antibodies immobilized on different-size magnetic beads in the future, this platform can be a potential candidate for a high-efficiency sensor array in clinical applications.
High-Sensitive FAM Labeled Aptasensor Based on Fe3O4/Au/g-C3N4 for the Detection of Sulfamethazine in Food Matrix
Authors: Yan Xueling , Yang Lulan , Tang Jiaming , Wen Xu , Chen Xingyue , Zheng Xiaoling , Chen Lingling , Li Jiaqi , Le Tao
Published: September 2022

In this study, we developed a fluorescent aptasensor based on Fe3O4/Au/g-C3N4 and a FAM-labeled aptamer (FAM-SMZ1S) against sulfamethazine (SMZ) for the specific and sensitive detection of SMZ in food matrix. The FAM-SMZ1S was adsorbed by the Fe3O4/Au/g-C3N4 via π–π stacking and electrostatic adsorption, serving as a basis for the ultrasensitive detection of SMZ. Molecular dynamics was used to explain the reasons why SMZ1S and SMZ were combined. This aptasensor presented sensitive recognition performance, with a limit of detection of 0.16 ng/mL and a linear range of 1–100 ng/mL. The recovery rate ranged from 91.6% to 106.8%, and the coefficient of variation (CV) ranged from 2.8% to 13.4%. In addition, we tested the aptasensor for the monitoring of SMZ in various matrix samples, and the results were well-correlated (R2 ≥ 0.9153) with those obtained for HPLC detection. According to these results, the aptasensor was sensitive and accurate, representing a potentially useful tool for the detection of SMZ in food matrix.
Experimental Induction of Intracranial Aneurysms in Rats: A New Model Utilizing a Genetic Modification within the EDNRA Gene
Authors: Lampmann Tim , Borger Valeri , Konczalla Jürgen , Gispert Suzana , Auburger Georg , Vatter Hartmut , Güresir Erdem
Published: September 2022

The rupture of an intracranial aneurysm (IA) leads to life-threatening subarachnoid hemorrhage. Aside from well-established risk factors, recently published genome-wide association studies of IA revealed the strong association of a common variant near the endothelin receptor type A (EDNRA) gene with IA risk. However, the role of EDNRA in the pathogenesis of IA remains unclear. The aim of this study was to investigate the influence of a genetic modification within the EDNRA gene on IA pathogenesis in a novel in vivo model. Adult wild-type Sprague–Dawley rats (WT rats) and genetically modified rats (EDNRA rats) were used for the induction of IA using arterial hypertension (HT). Animals were stratified into four groups: WT rats without (WT_CTL) and with induction of HT (WT + HT), as well as EDNRA rats without (EDNRA_CTL) and with induction of HT (EDNRA + HT). Blood pressure (BP) was observed for 12 weeks. After the observation period, cerebral arteries were analyzed for morphological (i.e., aneurysmal) changes as well as histological and functional changes by immunofluorescence and functional investigation. In the groups of rats with induction of HT, BP was higher in EDNRA + HT compared with that in WT + HT. No IAs were observed in WT_CTL and EDNRA_CTL but were found in WT + HT and EDNRA + HT. There was no histological difference in the immunofluorescence of EDNRA between all groups. Contractility and potency of endothelin-1 differed between the groups in functional investigation. In summary, we created a new model that is suitable for further studies for better understanding of the role of EDNRA in IA pathogenesis.
Fabrication and Characterization of Cement-Based Hybrid Concrete Containing Coir Fiber for Advancing Concrete Construction
Authors: Katman Herda Yati Binti , Khai Wong Jee , Bheel Naraindas , Kırgız Mehmet Serkan , Kumar Aneel , Benjeddou Omrane
Published: September 2022

Nowadays, the incorporation of natural fiber, such as coir fiber, to high-strength concrete has sparked a lot of attention in the construction materials industry. This is because coir fibers are significantly cheaper and more widely accessible than synthetic fibers. Natural fibers such as bamboo, flax, hemp, and coir have distinct microstructures and chemical compositions from cement-based materials. The physical and mechanical properties of natural fiber, such as coir fiber, are significantly correlated with fiber concentration and cellulose component. However, coir fiber has high stretching to failure, while bamboo, flax, and hemp fibers are very resistant to stress and increase stiffness. Based on these distinctive fiber qualities, it is anticipated that coir fiber would facilitate the development of cement-based materials for advanced concrete building applications. In this paper, coir fiber-reinforced cement-based concretes were evaluated in terms of workability, compressive strength, flexural strength, splitting tensile strength, modulus of elasticity, and permeability. The relationship between strength and fiber content was analyzed to understand the impact of coir fiber on the properties of coir fiber-reinforced cement-based concrete. Based on the results obtained, it is determined that 2% coir fiber modification offers the highest compressive strength, splitting tensile strength, and flexural strength. Moreover, the modulus of elasticity is increased, and the permeability is plummeted by the volume fractions of coir fiber 1%, 2%, and 3% because the blending of coir fiber has a bridging and dispersing mechanism of the force-carrying capacity in concrete. In conclusion, coir fiber might be a viable choice for improving the strength and durability of concrete. Therefore, the sparing use of coir fiber presented in this research can be implemented for the manufacturing of concrete in the future.
Extracellular Vesicles: A Novel Tool in Nanomedicine and Cancer Treatment
Authors: Stavrou Aikaterini , Ortiz Angelica
Published: September 2022

Extracellular vesicles are membrane-bound vesicles released by cells to mediate intercellular communication and homeostasis. Various external stimuli as well as inherent abnormalities result in alterations in the extracellular vesicle milieu. Changes to cells result in alterations in the content of the extracellular vesicle biogenesis, which may affect proximal and distal cells encountering these altered extracellular vesicles. Therefore, the examination of changes in the extracellular vesicle signature can be used to follow disease progression, reveal possible targets to improve therapy, as well as to serve as mediators of therapy. Furthermore, recent studies have developed methods to alter the cargo of extracellular vesicles to restore normal function or deliver therapeutic agents. This review will examine how extracellular vesicles from cancer cells differ from normal cells, how these altered extracellular vesicles can contribute to cancer progression, and how extracellular vesicles can be used as a therapeutic agent to target cancer cells and cancer-associated stroma. Here we present extracellular vesicles as a novel tool in nanomedicine.
Inhibition of KRAS, MEK and PI3K Demonstrate Synergistic Anti-Tumor Effects in Pancreatic Ductal Adenocarcinoma Cell Lines
Authors: Ma Yixuan , Schulz Benjamin , Trakooljul Nares , Al Ammar Moosheer , Sekora Anett , Sender Sina , Hadlich Frieder , Zechner Dietmar , Weiss Frank , Lerch Markus , Jaster Robert , Junghanss Christian , Murua Escobar Hugo
Published: September 2022

Kirsten rat sarcoma virus (KRAS) mutations are widespread in pancreatic ductal adenocarcinoma (PDAC) and contribute significantly to tumor initiation, progression, tumor relapse/resistance, and prognosis of patients. Although inhibitors against KRAS mutations have been developed, this therapeutic approach is not routinely used in PDAC patients. We investigated the anti-tumor efficacy of two KRAS inhibitors BI-3406 (KRAS::SOS1 inhibitor) and sotorasib (KRAS G12C inhibitor) alone or in combination with MEK1/2 inhibitor trametinib and/or PI3K inhibitor buparlisib in seven PDAC cell lines. Whole transcriptomic analysis of combined inhibition and control groups were comparatively analyzed to explore the corresponding mechanisms of inhibitor combination. Both KRAS inhibitors and corresponding combinations exhibited cytotoxicity against specific PDAC cell lines. BI-3406 enhance the efficacy of trametinib and buparlisib in BXPC-3, ASPC-1 and MIA PACA-2, but not in CAPAN-1, while sotorasib enhances the efficacy of trametinib and buparlisib only in MIA PACA-2. The whole transcriptomic analysis demonstrates that the two triple-inhibitor combinations exert antitumor effects by affecting related cell functions, such as affecting the immune system, cell adhesion, cell migration, and cytokine binding. As well as directly involved in RAF/MEK/ERK pathway and PI3K/AKT pathway affect cell survival. Our current study confirmed inhibition of KRAS and its downstream pathways as a potential novel therapy for PDAC and provides fundamental data for in vivo evaluations.
Utility of the Diffusion Weighted Sequence in Gynecological Imaging: Review Article
Authors: Bonde Apurva , Andreazza Dal Lago Eduardo , Foster Bryan , Javadi Sanaz , Palmquist Sarah , Bhosale Priya
Published: September 2022

Functional imaging with diffusion-weighted imaging (DWI) is a complementary tool to conventional diagnostic magnetic resonance imaging sequences. It is being increasingly investigated to predict tumor response and assess tumor recurrence. We elucidate the specific technical modifications of DWI preferred for gynecological imaging, including the different b-values and planes for image acquisition. Additionally, we discuss the problems and potential pitfalls encountered during DWI interpretation and ways to overcome them. DWI has a wide range of clinical applications in malignant and non-malignant gynecological conditions. It provides supplemental information helpful in diagnosing and managing tubo-ovarian abscess, uterine fibroids, endometriosis, adnexal torsion, and dermoid. Similarly, DWI has diverse applications in gynecological oncology in diagnosis, staging, detection of recurrent disease, and tumor response assessment. Quantitative evaluation with apparent diffusion coefficient (ADC) measurement is being increasingly evaluated for correlation with various tumor parameters in managing gynecological malignancies aiding in preoperative treatment planning. Newer advanced DWI techniques of diffusion tensor imaging (DTI) and whole body DWI with background suppression (DWIBS) and their potential uses in pelvic nerve mapping, preoperative planning, and fertility-preserving surgeries are briefly discussed.
CAR-T-Cell Therapy for Solid Tumors Positive for Fibronectin Extra Domain B
Authors: Zhang Zhijie , Liu Chang , Yang Zhe , Yin Hongping
Published: September 2022

(1) Background: The lack of specific targets has slowed the progress of CAR-T in treating solid tumors. Recent studies have revealed that EDB-FN (fibronectin extra domain B) may be an effective target for CAR-T treatment of solid tumors; EDB-FN is expressed in tumor and embryonic tissues, and antibody–cytokine fusion proteins targeting EDB-FN have been developed. However, the therapeutic effects of BBz CAR-engineered T-cells targeting EDB-FN in solid tumors have not been evaluated. (2) Results: In this study, we constructed a BBz CAR construct targeting EDB-FN, and the CAR molecule was expressed on the surface of T-cells by lentiviral transduction. In vitro, CAR-T-cells can be activated to express perforin and granzyme and lyse EDB-positive cells (U-87 MG cells, A549 cells, and HUVECs) and have no toxicity to EDB-negative cells (MCF-7). Compared to T-cells, CAR-T-cells can release cytokines after coculture with EDB-positive cell lines. In vivo, CAR-T-cells inhibited the progression of U-87 MG subcutaneous tumors and significantly reduced the blood vessel density in tumor tissue compared to T-cells, without obvious toxicity to mouse tissues and organs. Furthermore, CAR-T-cells overexpressing BiTE targeting EDB-FN can significantly improve their antitumor activity in vitro. (3) Conclusions: These results demonstrate that CAR-T-cells have specific antitumor and angiogenic activities in vivo and in vitro, suggesting that EDB-FN may be a potential solid tumor target for CAR-T therapy.
Paper-Based Detection Device for Microenvironment Examination: Measuring Neurotransmitters and Cytokines in the Mice Acupoint
Authors: Hsiao I-Han , Liao Hsien-Yin , Cheng Chao-Min , Yen Chia-Ming , Lin Yi-Wen
Published: September 2022

(1) Background: The medical practice of acupuncture involves the insertion of a specialized stainless needle into a specific body point, often called an acupoint, to initiate a perceived phenomenon of de-qi sensation. Therefore, the term “de-qi” describes bodily sensations experienced by the recipient during acupuncture, which may include feelings of soreness, heaviness, fullness, numbness, and migration. However, while acupuncture treatments reportedly result in acupoint activation and an increased release of neurotransmitters or cytokines, detecting these substances released into the acupoint microenvironment is often missed or delayed in clinical and basic practice. (2) Methods: To address this situation, we employed a paper-based enzyme-linked immunosorbent assay method to examine acupoint environmental changes using minute volumes of easily accessible acupoint fluids. (3) Results: Our results indicated that while levels of adenosine triphosphate (ATP), interleukin-1β, interleukin-6, glutamate, substance P, and histamine were all increased in the experimental group following electroacupuncture (EA) treatment, contrary results were observed in the sham EA and transient receptor potential vanilloid 1 (Trpv1−/−) groups. Subsequently, TRPV1 and its associated molecules were augmented in mouse dorsal root ganglion, spinal cord, thalamus, and the somatosensory cortex, then examined by Western blotting and immunofluorescence techniques. Investigations revealed that these elevations were still unobserved in the sham EA or EA in the Trpv1−/− groups. Furthermore, results showed that while administering ATP could mimic EA function, it could be reversed by the ATP P2 receptor antagonist, suramin. (4) Conclusions: Our data provide novel information, indicating that changes in neurotransmitter and cytokine levels can offer insight into acupuncture mechanisms and clinical targeting.
Sugarcane Streak Mosaic Virus P1 Attenuates Plant Antiviral Immunity and Enhances Potato Virus X Infection in Nicotiana benthamiana
Authors: Zhang Kun , Xu Xiaowei , Guo Xiao , Ding Shiwen , Gu Tianxiao , Qin Lang , He Zhen
Published: September 2022

The sugarcane streak mosaic virus (SCSMV) is the most important disease in sugarcane produced in southern China. The SCSMV encoded protein 1 (P1SCSMV) is important in disease development, but little is known about its detailed functions in plant–virus interactions. Here, the differential accumulated proteins (DAPs) were identified in the heterologous expression of P1SCSMV via a potato virus X (PVX)-based expression system, using a newly developed four-dimensional proteomics approach. The data were evaluated for credibility and reliability using qRT-RCR and Western blot analyses. The physiological response caused by host factors that directly interacted with the PVX-encoded proteins was more pronounced for enhancing the PVX accumulation and pathogenesis in Nicotiana benthamiana. P1SCSMV reduced photosynthesis by damaging the photosystem II (PSII). Overall, P1SCSMV promotes changes in the physiological status of its host by up- or downregulating the expression of host factors that directly interact with the viral proteins. This creates optimal conditions for PVX replication and movement, thereby enhancing its accumulation levels and pathogenesis. Our investigation is the first to supply detailed evidence of the pathogenesis-enhancing role of P1SCSMV, which provides a deeper understanding of the mechanisms behind virus–host interactions.