bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of the Effects of Ag, Cu, ZnO and TiO2 Nanoparticles on the Expression Level of Oxidative Stress-Related Genes and the Activity of Antioxidant Enzymes in Escherichia coli, Bacillus cereus and Staphylococcus epidermidis
Authors: Metryka Oliwia , Wasilkowski Daniel , Mrozik Agnieszka
Published: April 2022

Although the molecular response of bacteria exposed to metal nanoparticles (NPs) is intensively studied, many phenomena related to their survival, metal uptake, gene expression and protein production are not fully understood. Therefore, this work aimed to study Ag-NPs, Cu-NPs, ZnO-NPs and TiO2-NPs-induced alterations in the expression level of selected oxidative stress-related genes in connection with the activity of antioxidant enzymes: catalase (CAT), peroxidase (PER) and superoxide dismutase (SOD) in Escherichia coli, Bacillus cereus and Staphylococcus epidermidis. The methodology used included: the extraction of total RNA and cDNA synthesis, the preparation of primers for selected housekeeping and oxidative stress genes, RT-qPCR reaction and the measurements of CAT, PER and SOD activities. It was established that the treatment of E. coli and S. epidermidis with NPs resulted mainly in the down-regulation of targeted genes, whilst the up-regulation of genes was confirmed in B. cereus. The greatest differences in the relative expression levels of tested genes occurred in B. cereus and S. epidermidis treated with TiO2-NPs, while in E. coli, they were observed under ZnO-NPs exposure. The changes found were mostly related to the expression of genes encoding proteins with PER and CAT-like activity. Among NPs, ZnO-NPs and Cu-NPs increased the activity of antioxidants in E. coli and B. cereus. In turn, TiO2-NPs had a major effect on enzymes activity in S. epidermidis. Considering all of the collected results for tested bacteria, it can be emphasised that the impact of NPs on the antioxidant system functioning was dependent on their type and concentration.
Extracellular Vesicles as an Index for Endothelial Injury and Cardiac Dysfunction in a Rodent Model of GDM
Authors: Kereliuk Stephanie M. , Xiao Fengxia , Burger Dylan , Dolinsky Vernon W.
Published: April 2022

Gestational diabetes mellitus (GDM) increases risk of adverse pregnancy outcomes and maternal cardiovascular complications. It is widely believed that maternal endothelial dysfunction is a critical determinant of these risks, however, connections to maternal cardiac dysfunction and mechanisms of pathogenesis are unclear. Circulating extracellular vesicles (EVs) are emerging biomarkers that may provide insights into the pathogenesis of GDM. We examined the impact of GDM on maternal cardiac and vascular health in a rat model of diet-induced obesity-associated GDM. We observed a >3-fold increase in circulating levels of endothelial EVs (p < 0.01) and von Willebrand factor (p < 0.001) in GDM rats. A significant increase in mitochondrial DNA (mtDNA) within circulating extracellular vesicles was also observed suggesting possible mitochondrial dysfunction in the vasculature. This was supported by nicotinamide adenine dinucleotide deficiency in aortas of GDM mice. GDM was also associated with cardiac remodeling (increased LV mass) and a marked impairment in maternal diastolic function (increased isovolumetric relaxation time [IVRT], p < 0.01). Finally, we observed a strong positive correlation between endothelial EV levels and IVRT (r = 0.57, p < 0.05). In summary, we observed maternal vascular and cardiac dysfunction in rodent GDM accompanied by increased circulating endothelial EVs and EV-associated mitochondrial DNA. Our study highlights a novel method for assessment of vascular injury in GDM and highlights vascular mitochondrial injury as a possible therapeutic target.
RNA and Protein Determinants Mediate Differential Binding of miRNAs by a Viral Suppressor of RNA Silencing Thus Modulating Antiviral Immune Responses in Plants
Authors: Pertermann Robert , Golbik Ralph Peter , Tamilarasan Selvaraj , Gursinsky Torsten , Gago-Zachert Selma , Pantaleo Vitantonio , Thondorf Iris , Behrens Sven-Erik
Published: April 2022

Many plant viruses express suppressor proteins (VSRs) that can inhibit RNA silencing, a central component of antiviral plant immunity. The most common activity of VSRs is the high-affinity binding of virus-derived siRNAs and thus their sequestration from the silencing process. Since siRNAs share large homologies with miRNAs, VSRs like the Tombusvirus p19 may also bind miRNAs and in this way modulate cellular gene expression at the post-transcriptional level. Interestingly, the binding affinity of p19 varies considerably between different miRNAs, and the molecular determinants affecting this property have not yet been adequately characterized. Addressing this, we analyzed the binding of p19 to the miRNAs 162 and 168, which regulate the expression of the important RNA silencing constituents Dicer-like 1 (DCL1) and Argonaute 1 (AGO1), respectively. p19 binds miRNA162 with similar high affinity as siRNA, whereas the affinity for miRNA168 is significantly lower. We show that specific molecular features, such as mismatches and ‘G–U wobbles’ on the RNA side and defined amino acid residues on the VSR side, mediate this property. Our observations highlight the remarkable adaptation of VSR binding affinities to achieve differential effects on host miRNA activities. Moreover, they show that even minimal changes, i.e., a single base pair in a miRNA duplex, can have significant effects on the efficiency of the plant antiviral immune response.
Androgens Upregulate Pathogen-Induced Placental Innate Immune Response
Authors: Vancolen Seline , Ayash Taghreed , Segura Mariela , Allard Marie-Julie , Robaire Bernard , Sébire Guillaume
Published: April 2022

Group B Streptococcus (GBS) is a leading cause of placental infection, termed chorioamnionitis. Chorioamnionitis is associated with an increased risk of neurobehavioral impairments, such as autism spectrum disorders, which are more prominent in males than in female offspring. In a pre-clinical model of chorioamnionitis, a greater inflammatory response was observed in placenta associated with male rather than female fetuses, correlating with the severity of subsequent neurobehavioral impairments. The reason for this sex difference is not understood. Our hypothesis is that androgens upregulate the placental innate immune response in male fetuses. Lewis dams were injected daily from gestational day (G) 18 to 21 with corn oil (vehicle) or an androgen receptor antagonist (flutamide). On G 19, dams were injected with saline (control) or GBS. Maternal, fetal sera and placentas were collected for protein assays and in situ analyses. Our results showed that while flutamide alone had no effect, a decrease in placental concentration of pro-inflammatory cytokines and infiltration of polymorphonuclear cells was observed in flutamide/infected compared to vehicle/infected groups. These results show that androgens upregulate the placental innate immune response and thus may contribute to the skewed sex ratio towards males observed in several developmental impairments resulting from perinatal infection/inflammation.
Optimization of Fibrin Scaffolds to Study Friction in Cultured Mesothelial Cells
Authors: Bodega Francesca , Sironi Chiara , Zocchi Luciano , Porta Cristina
Published: April 2022

To study the friction of cell monolayers avoiding damage due to stress concentration, cells can be cultured on fibrin gels, which have a structure and viscoelasticity similar to that of the extracellular matrix. In the present research, we studied different gel compositions and surface coatings in order to identify the best conditions to measure friction in vitro. We examined the adhesion and growth behavior of mesothelial cell line MET-5A on fibrin gels with different fibrinogen concentrations (15, 20, and 25 mg/mL) and with different adhesion coatings (5 μg/mL fibronectin, 10 μg/mL fibronectin, or 10 μg/mL fibronectin + 10 μg/mL collagen). We also investigated whether different substrates influenced the coefficient of friction and the ability of cells to stick to the gel during sliding. Finally, we studied the degradation rates of gels with and without cells. All substrates tested provided a suitable environment for the adherence and proliferation of mesothelial cells, and friction measurements did not cause significant cell damage or detachment. However, in gels with a lower fibrinogen concentration, cell viability was higher and cell detachment after friction measurement was lower. Fibrinolysis was negligible in all the substrates tested.
Transcriptomic Profiling of Peripheral Edge of Lesions to Elucidate the Pathogenesis of Psoriasis Vulgaris
Authors: Boonpethkaew Suphagan , Meephansan Jitlada , Jumlongpim Onjira , Tangtanatakul Pattarin , Soonthornchai Wipasiri , Wongpiyabovorn Jongkonnee , Vipanurat Ratchanee , Komine Mayumi
Published: April 2022

Elucidating transcriptome in the peripheral edge of the lesional (PE) skin could provide a better understanding of the molecules or signalings that intensify inflammation in the PE skin. Full-thickness biopsies of PE skin and uninvolved (UN) skin were obtained from psoriasis patients for RNA-seq. Several potential differentially expressed genes (DEGs) in the PE skin compared to those in the UN skin were identified. These DEGs enhanced functions such as angiogenesis, growth of epithelial tissue, chemotaxis and homing of cells, growth of connective tissues, and degranulation of myeloid cells beneath the PE skin. Moreover, the canonical pathways of IL-17A, IL-6, and IL-22 signaling were enriched by the DEGs. Finally, we proposed that inflammation in the PE skin might be driven by the IL-36/TLR9 axis or IL-6/Th17 axis and potentiated by IL-36α, IL-36γ, IL-17C, IL-8, S100A7, S100A8, S100A9, S100A15, SERPINB4, and hBD-2. Along with IL-36α, IL-17C, and IκBζ, ROCK2 could be an equally important factor in the pathogenesis of psoriasis, which may involve self-sustaining circuits between innate and adaptive immune responses via regulation of IL-36α and IL-36γ expression. Our finding provides new insight into signaling pathways in PE skin, which could lead to the discovery of new psoriasis targets.
Preservation of Organs to Be Transplanted: An Essential Step in the Transplant Process
Authors: Lepoittevin Maryne , Giraud Sébastien , Kerforne Thomas , Barrou Benoit , Badet Lionel , Bucur Petru , Salamé Ephrem , Goumard Claire , Savier Eric , Branchereau Julien , Battistella Pascal , Mercier Olaf , Mussot Sacha , Hauet Thierry , Thuillier Raphael
Published: April 2022

Organ transplantation remains the treatment of last resort in case of failure of a vital organ (lung, liver, heart, intestine) or non-vital organ (essentially the kidney and pancreas) for which supplementary treatments exist. It remains the best alternative both in terms of quality-of-life and life expectancy for patients and of public health expenditure. Unfortunately, organ shortage remains a widespread issue, as on average only about 25% of patients waiting for an organ are transplanted each year. This situation has led to the consideration of recent donor populations (deceased by brain death with extended criteria or deceased after circulatory arrest). These organs are sensitive to the conditions of conservation during the ischemia phase, which have an impact on the graft’s short- and long-term fate. This evolution necessitates a more adapted management of organ donation and the optimization of preservation conditions. In this general review, the different aspects of preservation will be considered. Initially done by hypothermia with the help of specific solutions, preservation is evolving with oxygenated perfusion, in hypothermia or normothermia, aiming at maintaining tissue metabolism. Preservation time is also becoming a unique evaluation window to predict organ quality, allowing repair and/or optimization of recipient choice.
Quantification of 8-oxoG in Plant Telomeres
Authors: Castillo-González Claudia , Barbero Barcenilla Borja , Young Pierce G. , Hall Emily , Shippen Dorothy E.
Published: April 2022

Chemical modifications in DNA impact gene regulation and chromatin structure. DNA oxidation, for example, alters gene expression, DNA synthesis and cell cycle progression. Modification of telomeric DNA by oxidation is emerging as a marker of genotoxic damage and is associated with reduced genome integrity and changes in telomere length and telomerase activity. 8-oxoguanine (8-oxoG) is the most studied and common outcome of oxidative damage in DNA. The G-rich nature of telomeric DNA is proposed to make it a hotspot for oxidation, but because telomeres make up only a tiny fraction of the genome, it has been difficult to directly test this hypothesis by studying dynamic DNA modifications specific to this region in vivo. Here, we present a new, robust method to differentially enrich telomeric DNA in solution, coupled with downstream methods for determination of chemical modification. Specifically, we measure 8-oxoG in Arabidopsis thaliana telomeres under normal and oxidative stress conditions. We show that telomere length is unchanged in response to oxidative stress in three different wild-type accessions. Furthermore, we report that while telomeric DNA comprises only 0.02–0.07% of the total genome, telomeres contribute between 0.2 and 15% of the total 8-oxoG. That is, plant telomeres accumulate 8-oxoG at levels approximately 100-fold higher than the rest of the genome under standard growth conditions. Moreover, they are the primary targets of further damage upon oxidative stress. Interestingly, the accumulation of 8-oxoG in the chromosome body seems to be inversely proportional to telomere length. These findings support the hypothesis that telomeres are hotspots of 8-oxoG and may function as sentinels of oxidative stress in plants.
Small GTPases and Their Regulators: A Leading Road toward Blood Vessel Development in Zebrafish
Authors: Urade Ritesh , Chiu Yan-Hui , Chiu Chien-Chih , Wu Chang-Yi
Published: April 2022

Members of the Ras superfamily have been found to perform several functions leading to the development of eukaryotes. These small GTPases are divided into five major subfamilies, and their regulators can “turn on” and “turn off” signals. Recent studies have shown that this superfamily of proteins has various roles in the process of vascular development, such as vasculogenesis and angiogenesis. Here, we discuss the role of these subfamilies in the development of the vascular system in zebrafish.
Reactive and Senescent Astroglial Phenotypes as Hallmarks of Brain Pathologies
Authors: Lazic Andrijana , Balint Vanda , Stanisavljevic Ninkovic Danijela , Peric Mina , Stevanovic Milena
Published: April 2022

Astrocytes, as the most abundant glial cells in the central nervous system, are tightly integrated into neural networks and participate in numerous aspects of brain physiology and pathology. They are the main homeostatic cells in the central nervous system, and the loss of astrocyte physiological functions and/or gain of pro-inflammatory functions, due to their reactivation or cellular senescence, can have profound impacts on the surrounding microenvironment with pathological outcomes. Although the importance of astrocytes is generally recognized, and both senescence and reactive astrogliosis have been extensively reviewed independently, there are only a few comparative overviews of these complex processes. In this review, we summarize the latest data regarding astrocyte reactivation and senescence, and outline similarities and differences between these phenotypes from morphological, functional, and molecular points of view. A special focus has been given to neurodegenerative diseases, where these phenotypic alternations of astrocytes are significantly implicated. We also summarize current perspectives regarding new advances in model systems based on astrocytes as well as data pointing to these glial cells as potential therapeutic targets.
Allopregnanolone Promotes Migration and Invasion of Human Glioblastoma Cells through the Protein Tyrosine Kinase c-Src Activation
Authors: Zamora-Sánchez Carmen J. , Bello-Alvarez Claudia , Rodríguez-Dorantes Mauricio , Camacho-Arroyo Ignacio
Published: April 2022

Glioblastomas (GBs) are the most aggressive and common primary malignant brain tumors. Steroid hormone progesterone (P4) and its neuroactive metabolites, such as allopregnanolone (3α-THP) are synthesized by neural, glial, and malignant GB cells. P4 promotes cellular proliferation, migration, and invasion of human GB cells at physiological concentrations. It has been reported that 3α-THP promotes GB cell proliferation. Here we investigated the effects of 3α-THP on GB cell migration and invasion, the participation of the enzymes involved in its metabolism (AKR1C1-4), and the role of the c-Src kinase in 3α-THP effects in GBs. 3α-THP 100 nM promoted migration and invasion of U251, U87, and LN229 human-derived GB cell lines. We observed that U251, LN229, and T98G cell lines exhibited a higher protein content of AKR1C1-4 than normal human astrocytes. AKR1C1-4 silencing did not modify 3α-THP effects on migration and invasion. 3α-THP activated c-Src protein at 10 min (U251 cells) and 15 min (U87 and LN229 cells). Interestingly, the pharmacological inhibition of c-Src decreases the promoting effects of 3α-THP on cell migration and invasion. Together, these data indicate that 3α-THP promotes GB migration and invasion through c-Src activation.
Stress-Induced Depression and Alzheimer’s Disease: Focus on Astrocytes
Authors: Dolotov Oleg V. , Inozemtseva Ludmila S. , Myasoedov Nikolay F. , Grivennikov Igor A.
Published: April 2022

Neurodegenerative diseases and depression are multifactorial disorders with a complex and poorly understood physiopathology. Astrocytes play a key role in the functioning of neurons in norm and pathology. Stress is an important factor for the development of brain disorders. Here, we review data on the effects of stress on astrocyte function and evidence of the involvement of astrocyte dysfunction in depression and Alzheimer’s disease (AD). Stressful life events are an important risk factor for depression; meanwhile, depression is an important risk factor for AD. Clinical data indicate atrophic changes in the same areas of the brain, the hippocampus and prefrontal cortex (PFC), in both pathologies. These brain regions play a key role in regulating the stress response and are most vulnerable to the action of glucocorticoids. PFC astrocytes are critically involved in the development of depression. Stress alters astrocyte function and can result in pyroptotic death of not only neurons, but also astrocytes. BDNF-TrkB system not only plays a key role in depression and in normalizing the stress response, but also appears to be an important factor in the functioning of astrocytes. Astrocytes, being a target for stress and glucocorticoids, are a promising target for the treatment of stress-dependent depression and AD.
Membrane Lipid Replacement with Glycerolphospholipids Slowly Reduces Self-Reported Symptom Severities in Chemically Exposed Gulf War Veterans
Authors: Nicolson Garth L. , Breeding Paul C.
Published: April 2022

Background: Chemically exposed veterans of the 1991 Gulf War have few options for treatment of conditions and symptoms related to their chemical exposures. Membrane Lipid Replacement (MLR) with oral membrane glycerolphospholipids is a safe and effective method for slowly removing hydrophobic organic molecules from tissues, while enhancing mitochondrial function and decreasing the severity of certain signs and symptoms associated with multi-symptom illnesses. Methods: A preliminary open-label study utilizing 20 male veterans who were deployed to combat areas, exposed to environmental toxic chemicals and subsequently diagnosed with Gulf War Illnesses (GWI) were utilized. These subjects took 6 g per day oral glycerolphospholipids for 6 months, and the severities of over 100 signs and symptoms were self-reported at various times using illness survey forms. Results: In the sixteen patients that fully complied and completed the study, there were gradual and significant reductions of symptom severities in categories related to fatigue, pain, musculoskeletal, nasopharyngeal, breathing, vision, sleep, balance, and urinary, gastrointestinal and chemical sensitivities. There were no adverse incidents during the study, and the all-natural oral study supplement was extremely well tolerated. Conclusions: MLR with oral glycerolphospholipids appears to be a simple, safe and potentially effective method of slowly reducing the severities of multiple symptoms in chemically exposed veterans.
Correlation of Anti-HLA IgA Alloantibodies and Fc Receptor Motives with Kidney Allograft Survival
Authors: Arnold Marie-Luise , Steffen Ulrike , Wiesener Michael , Bach Christian , Spriewald Bernd M. , Lindemann Monika
Published: April 2022

Immunoglobulin A (IgA) is the most abundant antibody isotype in humans and anti-HLA IgA was found in sera of transplant recipients. Focusing on patients awaiting kidney re-transplantation, we tested the impact of anti-HLA-class I/II IgA antibodies on graft survival. We analyzed 276 patients with and 238 without allograft failure. Eight motives of the Fcα receptor (FCAR) and Fcγ receptor were analyzed in patients with allograft failure. The distribution of anti-HLA IgA1/A2 and IgG antibodies differed significantly (p < 0.0001) between both patient groups, and IgA1 plus IgA2 antibodies were more abundant in patients with allograft failure. Allograft survival was significantly impaired if anti-HLA-class I plus II IgA was present, in the first 105 months (9 years) of follow-up (median of 43 vs. >105 months, p = 0.007). Patients with anti-HLA IgA and IgG vs. anti-HLA IgG only had a significantly shorter allograft survival within that follow-up period (88 vs. >105 months, p = 0.008). Moreover, allograft survival was shorter (p = 0.02) in carriers of GG vs. AA + AG genotypes of FCAR rs16986050. Thus, the presence of anti-HLA IgA plus IgG vs. IgG only was associated with shorter kidney allograft survival and FCAR motives may impact on graft survival.
Evaluating the Performance of Lateritic Soil Stabilized with Cement and Biomass Bottom Ash for Use as Pavement Materials
Authors: Iyaruk Arsit , Promputthangkoon Panu , Lukjan Arun
Published: April 2022

From the perspective of sustainable waste management and its environmental impact, waste biomass bottom ash (BA) remains problematic and challenging to use as a recycling material for civil engineering infrastructures. This study evaluated the performance of lateritic soil (LS), stabilized with cement and biomass BA, as a subbase material. BA has been considered a replacement material in LS prior to the introduction of hydraulic cement stabilization means. The geotechnical engineering tests comprised the modified Proctor test, the California Bearing Ratio (CBR) test, and the unconfined compression test. X-ray fluorescence (XRF) and X-ray diffraction (XRD) tests were conducted to investigate the mineralogical properties of the stabilized soil samples. The leachate test was performed with a permeability mold to measure the release of heavy metals. Finally, the benefits of using the stabilized subbase material were assessed using the mechanistic–empirical (M–E) pavement design approach. Based on the results obtained, the strength and stiffness characteristics of the stabilized soils indicate that the efficiency of the mix satisfied the Thailand highway specification. The admixture of 80% BA and 5% cement is suggested for use as a soil–cement subbase material for flexible pavements, due to its good engineering and environmental properties. The results of the M–E design demonstrate the effectiveness of the stabilized soil presented herein. The study’s outcomes are predicted to promote the utilization of waste BA as a promising pavement material.
Serum Leucine-Rich Alpha-2 Glycoprotein in Quiescent Crohn’s Disease as a Potential Surrogate Marker for Small-Bowel Ulceration detected by Capsule Endoscopy
Authors: Omori Teppei , Sasaki Yu , Koroku Miki , Murasugi Shun , Yonezawa Maria , Nakamura Shinichi , Tokushige Katsutoshi
Published: April 2022

Background: Small bowel (SB) lesions in quiescent Crohn’s disease (CD) are sometimes not identified by clinical activity or existing markers. We investigated the usefulness of a novel biomarker, leucine-rich α2-glycoprotein (LRG), for screening for the presence of SB ulcerative lesions detected by small-bowel capsule endoscopy (SBCE). Methods: We examined patients with a Crohn’s Disease Activity Index (CDAI) value < 150 and a C-reactive protein (CRP) value < 0.5 mg/dL with SB or SB colonic CD. The presence of small-bowel ulcerative lesions (≥0.5 cm) was grouped by SBCE results, and we then compared the groups’ LRG value to establish a cutoff value for screening for the presence of lesions. Results: In 40 patients with CD, the LRG values differed significantly between the patients with and without SB ulcerative lesions (Ul + 14.1 (2.1–16.5) μg/mL vs. Ul − 12.3 (9.3–13.5) μg/mL; p = 0.0105). The respective cutoff LRG values for the presence of SB ulcerative lesions was 14 μg/mL (areas under the ROC curve 0.77), with sensitivity 63.6%, specificity 82.8%, positive predictive values 58.3%, negative predictive values 85.7%, and accuracy 78%. Conclusion: These results indicate that LRG may be useful in predicting the presence of SB inflammation associated in patients with CD with CRP < 0.5 mg/dL and CDAI < 150, and in selecting patients for SBCE.
Prostatic Abscess Caused by Klebsiella pneumoniae: A 6-Year Single-Center Study
Authors: Hwang Joo-Hee , Hwang Jeong-Hwan , Lee Seung Yeob , Lee Jaehyeon
Published: April 2022

Hypervirulent Klebsiella pneumoniae (hvKp) is an important strain that can cause multiple organ infections. Although hvKp infection cases are increasing, there is limited information on the prostatic abscesses caused by K. pneumoniae. Furthermore, the clinical significance of hvKp associated with K1 or K2 capsular types or virulence genes in prostatic abscesses remains unclear. Therefore, we aimed to elucidate the clinical and microbiological characteristics of prostatic abscesses caused by K. pneumoniae in relation to various virulence genes. A retrospective study was performed at a 1200-bed tertiary hospital between January 2014 and December 2019. Patients diagnosed with prostatic abscesses with K. pneumoniae isolated from blood, urine, pus, or tissue cultures were enrolled in this study. Our results demonstrate that 30.3% (10/33) of the prostatic abscesses were caused by K. pneumoniae. All strains isolated from patients with prostatic abscesses due to K. pneumoniae were the K1 capsular type, and eight patients (80.0%) carried rmpA and iutA genes that identified hvKp. These findings suggest that hvKp is an important pathogen in prostatic abscesses. Therefore, when treating patients with K. pneumoniae prostatic abscesses, attention should be paid to the characteristics of hvKp, such as bacteremia, multiorgan abscess formation, and metastatic spread.
Disturbance Observer-Based Double-Loop Sliding-Mode Control for Trajectory Tracking of Work-Class ROVs
Authors: Huang Bolun , Yang Qi
Published: April 2022

The open-frame structure of work-class ROVs results in significant model uncertainties, and its motion is strongly disturbed by the umbilical cable. To address these problems, this article developed a nonlinear disturbance observer-based super-twisting double-loop sliding-mode control (NDO-STDSMC) method to achieve trajectory tracking control of work-class ROVs with system uncertainties and external disturbances. First, a new outer-loop controller with a novel reaching law is designed to increase the convergence rate compared with the existing double-loop sliding-mode control (DSMC). Second, an inner-loop controller that combines the advantages of the super-twisting sliding-mode scheme is proposed to guarantee the tracking error converges to zero in finite time. Then, a nonlinear disturbance observer is designed to estimate and compensate for the system uncertainties and external disturbances. The stability of the overall control system is proven by the Lyapunov approach. Finally, comprehensive simulation studies on trajectory tracking control of work-class ROVs are provided to demonstrate the efficiency of the proposed NDO-STDSMC method and its superiority over existing DSMC and STDSMC methods.
Diagnosing Onychomycosis: What’s New?
Authors: Gupta Aditya K. , Hall Deanna C. , Cooper Elizabeth A. , Ghannoum Mahmoud A.
Published: April 2022

An overview of the long-established methods of diagnosing onychomycosis (potassium hydroxide testing, fungal culture, and histopathological examination) is provided followed by an outline of other diagnostic methods currently in use or under development. These methods generally use one of two diagnostic techniques: visual identification of infection (fungal elements or onychomycosis signs) or organism identification (typing of fungal genus/species). Visual diagnosis (dermoscopy, optical coherence tomography, confocal microscopy, UV fluorescence excitation) provides clinical evidence of infection, but may be limited by lack of organism information when treatment decisions are needed. The organism identification methods (lateral flow techniques, polymerase chain reaction, MALDI-TOF mass spectroscopy and Raman spectroscopy) seek to provide faster and more reliable identification than standard fungal culture methods. Additionally, artificial intelligence methods are being applied to assist with visual identification, with good success. Despite being considered the ‘gold standard’ for diagnosis, clinicians are generally well aware that the established methods have many limitations for diagnosis. The new techniques seek to augment established methods, but also have advantages and disadvantages relative to their diagnostic use. It remains to be seen which of the newer methods will become more widely used for diagnosis of onychomycosis. Clinicians need to be aware of the limitations of diagnostic utility calculations as well, and look beyond the numbers to assess which techniques will provide the best options for patient assessment and management.
Levodopa-Carbidopa Intestinal Gel Improves Symptoms of Orthostatic Hypotension in Patients with Parkinson’s Disease—Prospective Pilot Interventional Study
Authors: Stanková Simona , Straka Igor , Košutzká Zuzana , Valkovič Peter , Minár Michal
Published: April 2022

Parkinson’s disease (PD) is currently considered progressive neurodegeneration of both the central and peripheral nervous systems. Widespread neuropathological changes lead to a complex clinical presentation with typical motor (hypokinesia, tremor, and rigidity) and various nonmotor symptoms. Orthostatic hypotension is one of the most disabling nonmotor features contributing to increased morbidity and mortality and decreased quality of life (QoL). Our study aimed to disclose the effect of a continuous infusion of levodopa-carbidopa intestinal gel (LCIG) on symptoms of orthostatic hypotension. Nine patients indicated for LCIG and eight matched patients on optimized medical treatment (OMT) were examined with scales for orthostatic symptoms (SCOPA-AUT), nonmotor symptoms and motor fluctuations (MDS-UPDRS), and QoL (PDQ39) at both baseline and after six months. The scores of “light-headedness after standing” and “fainting” decreased in the LCIG group compared to the OMT group. Treatment with LCIG was associated with a significantly higher decrease in the score of “light-headedness after standing”. Change in the PDQ39 correlated positively with fluctuation improvement and with change in the scores of both “light-headedness” and “fainting”. LCIG treatment improved symptoms of orthostatic hypotension in patients with PD mainly by a reduction in motor complications. Decreased severity in both motor and nonmotor fluctuations was connected also with improved QoL. Continuous treatment with LCIG should be considered not only in the case of severe motor fluctuation but also in patients with nonmotor fluctuations responsive to dopaminergic treatment.
Modified Geometry of 106Ru Asymmetric Eye Plaques to Improve Dosimetric Calculations in Ophthalmic Brachytherapy
Authors: Miras Héctor , Terrón José Antonio , Bertolet Alejandro , Leal Antonio
Published: April 2022

Ru/Rh asymmetric plaques for ophthalmic brachytherapy have special geometric designs with a cutout intended to prevent irradiation of critical ocular structures proximal to the tumor. In this work, we present new geometric models for PENELOPE+PenEasy Monte Carlo simulations of these applicators, differing from the vendor-reported geometry, that better match their real geometry to assess their dosimetric impact. Simulation results were benchmarked to experimental dosimetric data from radiochromic film measurements, data provided by the manufacturer in the calibration certificates, and other experimental results published in the literature, obtaining, in all cases, better agreement with the modified geometries. The clinical impact of the new geometric models was evaluated by simulating real clinical cases using patient-specific eye models. The cases calculated using the modified geometries presented higher doses to the critical structures proximal to the cutout region. The modified geometric models presented in this work provide a more accurate representation of the asymmetric plaques, greatly improving the agreement between Monte Carlo calculations and experimental measurements. Lack of consideration of accurate geometric models has been shown to be translated into notable increases in dose to organs at risk in clinical cases.
Optimization of Preoperative Lymph Node Staging in Patients with Muscle-Invasive Bladder Cancer Using Radiomics on Computed Tomography
Authors: Starmans Martijn P. A. , Ho Li Shen , Smits Fokko , Beije Nick , de Kruijff Inge , de Jong Joep J. , Somford Diederik M. , Boevé Egbert R. , te Slaa Ed , Cauberg Evelyne C. C. , Klaver Sjoerd , van der Heijden Antoine G. , Wijburg Carl J. , van de Luijtgaarden Addy C. M. , van Melick Harm H. E. , Cauffman Ella , de Vries Peter , Jacobs Rens , Niessen Wiro J. , Visser Jacob J. , Klein Stefan , Boormans Joost L. , van der Veldt Astrid A. M.
Published: April 2022

Approximately 25% of the patients with muscle-invasive bladder cancer (MIBC) who are clinically node negative have occult lymph node metastases at radical cystectomy (RC) and pelvic lymph node dissection. The aim of this study was to evaluate preoperative CT-based radiomics to differentiate between pN+ and pN0 disease in patients with clinical stage cT2-T4aN0-N1M0 MIBC. Patients with cT2-T4aN0-N1M0 MIBC, of whom preoperative CT scans and pathology reports were available, were included from the prospective, multicenter CirGuidance trial. After manual segmentation of the lymph nodes, 564 radiomics features were extracted. A combination of different machine-learning methods was used to develop various decision models to differentiate between patients with pN+ and pN0 disease. A total of 209 patients (159 pN0; 50 pN+) were included, with a total of 3153 segmented lymph nodes. None of the individual radiomics features showed significant differences between pN+ and pN0 disease, and none of the radiomics models performed substantially better than random guessing. Hence, CT-based radiomics does not contribute to differentiation between pN+ and pN0 disease in patients with cT2-T4aN0-N1M0 MIBC.
Were Culture and Heritage Important for the Resilience of Tourism in the COVID-19 Pandemic?
Authors: Jurlin Krešimir
Published: April 2022

The unprecedented impact of the COVID-19 on the world tourism is clear and obvious. Still, modelling the impact on individual countries faces many problems from data availability to the multitude of underlying variables rather difficult to capture. This study used simple and multiple regression to research possible effects of the recent pandemic to the fall in the volume of tourism in 20 European countries, throughout the 20-month period. The results of this study were rather surprising showing that the relative fall in tourism cannot be explained only by incidence of COVID-19 by countries, while in multiple regression by adding the variables of distance of travel and composition of tourism by facilities coefficients of determination were very low. Adding variables of natural and cultural heritage as well as of cultural activities somewhat improved the baseline model with the best fitting variable of culture visits adding 11.8 percentage points to the explanatory power of the model, while culture employment and culture consumption added a possibly important 5.6 and 2.6 points, respectively. Although these findings are in line with recent literature of resilience and changes in tourism due to pandemic, a more thorough research is needed to further investigate these relations.
What’s Different about Bank Holding Companies?
Authors: Chami Ralph , Cosimano Thomas F. , Ma Jun , Rochon Celine
Published: April 2022

We develop a dynamic model of a BHC that encompasses both a trading desk and a loan desk, and explore the role of risk attitude and overleveraging by the trading desk. We trace the impact of monetary policy and market innovations on bank behavior in the presence of Basel III type regulations. We show that the value of the BHC is enhanced by operating both desks. We explore alternative regulatory remedies to ongoing efforts to ring-fence the proprietary trading business, and show that regulations that target bank governance can mitigate possible rogue trading and the overleveraging problem.
Primary and Secondary Progressive Aphasia in Posterior Cortical Atrophy
Authors: Brodeur Catherine , Belley Émilie , Deschênes Lisa-Marie , Enriquez-Rosas Adriana , Hubert Michelyne , Guimond Anik , Bilodeau Josée , Soucy Jean-Paul , Macoir Joël
Published: April 2022

Background: Posterior cortical atrophy (PCA) is a clinico-radiological syndrome characterized by a progressive decline in visuospatial/visuoperceptual processing. PCA is accompanied by the impairment of other cognitive functions, including language abilities. Methods: The present study focused on three patients presenting with language complaints and a clinical profile that was compatible with PCA. In addition to neurological and neuroimaging examinations, they were assessed with comprehensive batteries of neuropsychological and neurolinguistic tests. Results: The general medical profile of the three patients is consistent with PCA, although they presented with confounding factors, making diagnosis less clear. The cognitive profile of the three patients was marked by Balint and Gerstmann’s syndromes as well as impairments affecting executive functions, short-term and working memory, visuospatial and visuoperceptual abilities, and sensorimotor execution abilities. Their language ability was characterized by word-finding difficulties and impairments of sentence comprehension, sentence repetition, verbal fluency, narrative speech, reading, and writing. Conclusions: This study confirmed that PCA is marked by visuospatial and visuoperceptual deficits and reported evidence of primary and secondary language impairments in the three patients. The similarities of some of their language impairments with those found in the logopenic variant of primary progressive aphasia is discussed from neurolinguistic and neuroanatomical points of view.
An Insight into the Novel Immunotherapy and Targeted Therapeutic Strategies for Hepatocellular Carcinoma and Cholangiocarcinoma
Authors: Trifylli Eleni-Myrto , Koustas Evangelos , Papadopoulos Nikolaos , Sarantis Panagiotis , Aloizos Georgios , Damaskos Christos , Garmpis Nikolaos , Garmpi Anna , Karamouzis Michalis V.
Published: April 2022

Hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA) constitute highly malignant forms of primary liver cancers. Hepatocellular and bile duct carcinogenesis is a multiplex process, caused by various genetic and epigenetic alterations, the influence of environmental factors, as well as the implication of the gut microbiome, which was undervalued in the previous years. The molecular and immunological analysis of the above malignancies, as well as the identification of the crucial role of intestinal microbiota for hepatic and biliary pathogenesis, opened the horizon for novel therapeutic strategies, such as immunotherapy, and enhanced the overall survival of cancer patients. Some of the immunotherapy strategies that are either clinically applied or under pre-clinical studies include monoclonal antibodies, immune checkpoint blockade, cancer vaccines, as well as the utilization of oncolytic viral vectors and Chimeric antigen, receptor-engineered T (CAR-T) cell therapy. In this current review, we will shed light on the recent therapeutic modalities for the above primary liver cancers, as well as on the methods for the enhancement and optimization of anti-tumor immunity.
Effect of Pre-Oxidation Treatment on Corrosion Resistance of FeCoSiBPC Amorphous Alloy
Authors: Zhang Qian , Hui Xidong , Li Zongzhen , Zhang Guangqiang , Lin Jingcheng , Li Xuelian , Zheng Wei , Cao Xia , Zhou Shaoxiong
Published: April 2022

In this paper, the corrosion resistance of FeCoSiBPC amorphous alloy after pre-oxidation and non-oxidation heat treatment is investigated. The corrosion behaviors of Fe80Co3Si3B10P1C3 amorphous alloys in 1 mol/L NaCl solution were investigated by the electrochemical workstation. The pre-oxidation heat treatment can improve the corrosion resistance of FeCoSiBPC amorphous alloy through an increase in the Ecorr value from −0.736 to −0.668 V, which makes it easy to reach a passive state. The corroded morphology and products of amorphous alloys were tested by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The SEM/TEM analysis showed that, after pre-oxidation treatment, the oxide layer was divided into two layers: the inner layer was amorphous, the outer layer appeared crystalline, and the main oxide was Fe2O3. During the oxidation process, Co and P elements diffused from the inner layer to the outer layer, forming phosphorus and cobalt oxides with high corrosion resistance on the surface of the ribbon, thereby improving the corrosion resistance of the ribbon.
Study on the Cooling Effect of Asphalt Pavement Blended with Composite Phase Change Materials
Authors: Dai Ming , Wang Shiwan , Deng Jianbo , Gao Zhijie , Liu Zhiyun
Published: April 2022

To explore the cooling effect of phase change materials (PCM) on asphalt pavement, a numerical model of the coupled heat transfer process of a typical monolithic subgrade of the G7 Expressway in the eastern Tianshan mountain area was developed. Three types of paraffin materials (OP55E, OP52E, OP47E) were mixed in a 4:3:3 volume ratio and blended into the asphalt upper layer and overall asphalt layer at volume ratios of 5%, 10%, 15% and 20%. The cooling effect of different PCM addition schemes was simulated and analyzed, and the frequency and duration of asphalt pavement high temperature operation status were also measured. The results showed that: (1) Th addition of PCM in the asphalt layer can effectively reduce the frequency of pavement high temperature rutting damage. The number of days and average daily duration of high temperature on the road surface were both reduced. (2) The cooling effect was positively correlated with the PCM volume mixing ratio, and the temperature drop of the pavement also increased with the increase of the PCM blending ratio. As the PCM mixing ratio increased from 5% to 20%, the initial 75 °C pavement cooled by 1.49 °C and 4.66 °C, respectively, and the number of days and hours of pavement temperature over 70 °C decreased to 4 days and 3.3 h, respectively. (3) The cooling effect of the asphalt upper layer PCM scheme was greater at a small mixing ratio (5%), whereas the performance of the overall asphalt layer PCM blended scheme was effectively promoted by increasing the equivalent heat capacity of system under the large mixing ratio (20%).
The Effect of Heat Input, Annealing, and Deformation Treatment on Structure and Mechanical Properties of Electron Beam Additive Manufactured (EBAM) Silicon Bronze
Authors: Filippov Andrey , Shamarin Nikolay , Moskvichev Evgeny , Savchenko Nikolai , Kolubaev Evgeny , Khoroshko Ekaterina , Tarasov Sergei
Published: April 2022

Electron beam additive wire-feed manufacturing of Cu-3wt.%S-0.8wt.%Mn bronze thin wall on a stainless steel substrate has been carried out at heat input levels of 0.19, 0.25, and 0.31 kJ/mm. The microstructures of as-deposited metal ranged from low aspect ratio columnar with equiaxed grain layers to zig-zagged and high aspect ratio columnar, as depended on the heat input. Post-deposition annealing at 900 °C for 6 h resulted in recrystallization of the high aspect ratio columnar grains with further grain growth by boundary migration. Pre-deformation by 10% thickness reduction and then annealing at 900 °C for 6 h also allowed obtaining recrystallized grain structures with less fraction of twin boundaries but higher fraction of high-angle ones, as compared to those of only annealed sample. Pre-deformation and ensuing annealing allowed simultaneous increasing of the ultimate tensile strength and strain-to-fracture.
Durability and Improvement of Cement-Based Revetment Materials Serving in Subtidal, Intertidal, and Supratidal Environments
Authors: Sun Rui , Wang Dongmin , Wang Yiren , Zhang Lei , Gu Yue
Published: April 2022

To improve the durability of cement-based revetment materials serving in different positions relative to the water level, slag powder and polypropylene fibers were added into cement to prepare paste, mortar, and concrete. Based on three simulated experiments of high-humidity air, dry–wet cycles-coupled chloride erosion, and complete immersion-coupled chloride erosion, the half-year durability of cement-based revetment materials was investigated. An abundant amount of Ettringite containing chloride was formed in the pores of the cement, and its formation was accelerated by dry–wet cycles. Replacing 30% of cement by slag powder and adding 0.1 vol.% of polypropylene fibers helped concrete in the intertidal zone to obtain a compressive strength of 47.58 MPa after erosion, equal to 159% of the reference. Slag powder was found to induce cement to form Friedel’s salt and C-S-H with a more amorphous structure, increasing its chemical binding ability and physical adsorption ability to chloride ions, and reduce the chloride ions’ penetration depth of concrete from 22.5 to 12.6 mm. Polypropylene fibers controlled the direction of surface cracks to be perpendicular to the specimen’s sides. These findings lay a foundation for the design of high-durability cement-based revetment materials serving in costal environments.