bluuebunny/crossref_metadata_2025 huggingface dataset:
Green Synthesis of Piper nigrum Copper-based Nanoparticles: In-Silico Study and ADMET Analysis to Assess Their Antioxidant, Antibacterial, and Cytotoxic Effects
Authors: Anusha Y. , Rajesh Nambi , Vani M. Vidya , Khadri Habeeb , Mohammed Arifullah , Riazunnisa Khateef , Moussa Ashaimaa Y.
Published: December 2022

Nano-biotechnology gained popularity and interest among scientists since it allowed for the green manufacturing of nanoparticles by employing plants as reducing agents. This method was safe, cheap, reproducible, and eco-friendly. In this study, the therapeutic potential of Piper nigrum fruit was mixed with the antibacterial activity of metallic copper to produce copper nanoparticles. The synthesis of copper nanoparticles was indicated by a colour change from brown to blue. Physical characterisation of PN-CuNPs was done by using UV-vis spectroscopy, FTIR, SEM, EDX, XRD and Zeta analyser. PN-CuNPs exhibited potential antioxidant, antibacterial and cytotoxic activities. PN-CuNPs showed improved free radical scavenging activity in a concentration dependant manner, reaching a maximum of 92%, 90% and 86% with DPPH, H2O2 and PMA tests. The antibacterial zone of inhibition of PN-CuNPs was the highest against S. aureus (23 mm) and the lowest against E. coli (10 mm), respectively. In vitro cytotoxicity of PN-CuNPs was demonstrated against MCF-7 breast cancer cell lines. The green synthesis of P. nigrum fruit was an excellent approach to produce PN-CuNPs with significant biological properties. Furthermore, more than 50 components of Piper nigrum extract were selected and subjected to in-silico molecular docking using the C-Docker protocol in the binding pockets of glutathione reductase, E. coli DNA gyrase topoisomerase II and EGFR tyrosine to discover their druggability. Pipercyclobutanamide A (26), pipernigramide F (32) and pipernigramide G (33) scored the best Gibbs free energy 50.489, 51.9306, 58.615 Kcal/mol, respectively. The ADMET/TOPKAT analysis confirmed the favourable pharmacokinetics, pharmacodynamics and toxicity profiles of the three promising compounds.
Posture-Induced Changes in Intraocular Pressure after Ab Externo XEN45 Gel-Stent Implantation in Patients with Primary Open-Angle Glaucoma
Authors: Jin Hyung Nam , Nam Jeong Woo , Haowei Zhang , Sung Mi Sun , Park Sang Woo
Published: December 2022

Background: To investigate posture-induced changes in intraocular pressure (IOP) after ab externo XEN45 Gel-Stent implantation in patients with medically uncontrolled primary open-angle glaucoma (POAG).
Methods: This prospective study included thirty-two eyes with POAG that underwent XEN45 Gel-Stent implantation as a standalone procedure using an ab externo approach at Chonnam National University Hospital. IOP was measured sequentially in the sitting position, supine position, and lateral decubitus position (LDP) before and at 1, 2, 3, and 6 months after surgery using an iCare IC200 rebound tonometer. In the LDP, the eye with XEN45 Gel-Stent implantation was in the dependent position.
Results: IOP at each position was significantly reduced after XEN45 Gel-Stent implantation. Posture-induced changes in IOP were maintained during the follow-up. The range of postural IOP changes was reduced at 1 month; however, no significant change was observed after that point compared with baseline levels.
Conclusions: A XEN45 Gel-Stent inserted using the ab externo approach can reduce IOP in various body positions, but seems to have limited effects on posture-induced changes in IOP in patients with POAG.
CCAH: a CLIP-based Cycle Alignment Hashing method for unsupervised vision-text retrieval
Authors: Li Mingyong , Ma Longfei , Li Yewen , Ge Mingyuan
Published: December 2022

Due to the advantages of low storage cost and fast retrieval efficiency,
deep hashing methods are widely used in cross-modal retrieval. Images
are usually accompanied by corresponding text descriptions rather than
labels. Therefore, unsupervised methods have been widely concerned.
However, due to the modal divide and semantic differences, existing
unsupervised methods cannot adequately bridge the modal differences,
leading to suboptimal retrieval results. In this paper, we propose CLIPbased
Cycle Alignment Hashing for unsupervised vision-text retrieval
(CCAH), which aims to exploit the semantic link between the original
features of modalities and the reconstructed features. Firstly we design
a modal cyclic interaction method that aligns semantically within intramodality,
where one modal feature reconstructs another modal feature,
thus taking full account of the semantic similarity between intra-modal
and inter-modal relationships. Secondly, introducing GAT into crossmodal
retrieval tasks. Consider the influence of text neighbour nodes
and add attention mechanisms to capture the global features of text
modalities. Thirdly, Fine-grained extraction of image features using the
CLIP visual coder. Finally, hash encoding is learning through hash functions.
The experiments demonstrate on three widely used datasets that
our proposed CCAH achieves satisfactory results in total retrieval accuracy.
Our code can be found at :https://github.com/CQYIO/CCAH.git.
The lower in vivo osteogenicity of adipose tissue-derived stem cells correlates with a higher innate immune response
Authors: Maroquenne Manon , Bourguignon Marianne , Larochette Nathanael , Hafci Hanane El , Margottin Morgane , Potier Esther , Logeart-Avramoglou Delphine
Published: December 2022

Background: Adipose tissue-derived mesenchymal stem cells (ATSCs) have been used as an alternative to bone marrow-derived mesenchymal stem cells (BMSCs) for bone tissue engineering applications. However, the ability of ATSCs to promote new bone formation remains lower than that of BMSCs. The aim of this study was to investigate the mechanisms underlying osteogenicity differences between human ATSCs and BMSCs in tissue-engineered constructs, focusing on the effects of the innate immune response on this process.
Methods: In vivo bone formation induced by transplanted human BMSCs and ATSCs combined with ceramic granules was evaluated in an ectopic mouse model. Explants were analyzed by micro-computerized tomographic and histology analyses. Kinetic analyses of both the expressed human and murine genes pertaining to osteogenesis and inflammatory response in tissue constructs explanted at 0, 7, 14, and 28 days post-implantation were performed. The gene expression and secretome profiles of pro-inflammatory cytokines/chemokines in both ATSC and BMSC were analyzed.
Results: In contrast to ATSC-containing constructs, which did not induce bone formation, BMSC constructs consistently did so. Implanted BMSCs, concomitantly with host murine progenitors, differentiated into the osteogenic lineage early post-implantation. In contrast, ATSCs differentiated later, when few implanted viable cells remained post implantation, while the host murine cells did not differentiate. Concomitant and transient upregulation of some human and murine inflammatory genes in the ATSC-constructs was also observed early post-implantation. The high level of cytokine production by the ATSCs was confirmed at the gene- and protein-levels before implantation. Analysis of the immune cell recruitment within the constructs post-implantation showed higher numbers of multinuclear giant cells as well as M1 macrophages in the ATSC- constructs than in the BMSC-constructs.
Conclusions: ATSCs are a transient source of inflammatory cytokines promoting a transient immune response post implantation; this milieu correlates with impaired osteogenic differentiation of both the implanted ATSCs and the host osteoprogenitor cells. These data provide new insight regarding use of ATSCs for bone regeneration applications, while acknowledging the substantial potential of BMSCs for bone repair.
Lepidoptera as a tool for human disturbance assessment on biodiversity in Choke Mountains, Ethiopia
Authors: TUJUBA Tesfu Fekensa , Simonetto Anna , (Prof.) Gianni Gilioli , (Prof.) Andrea Sciarretta
Published: December 2022

Background:
Recent studies indicated that frequent anthropogenic disturbances in tropical countries are primary drivers of the reduction in community diversity and local extinction of many insect taxa, including Lepidoptera. Biodiversity assessment provides information on the responses of different taxa to these anthropogenic changes, useful to develop evidence-based conservation strategies to reduce biodiversity loss. We assessed the impact of anthropogenic disturbances on lepidopteran assemblages across five different land uses (forest fragments, crop fields, Pasture land, rural settlement and undisturbed natural forest) in Choke Mountains, Ethiopia. Automatic light traps have been used in 16 sites, in 3 sites manual samplings have been performed. A total of 4,559 moths representing 14 families and 339 species were sampled. The highest diversity was obtained from the Natural Forest (269 species), followed by Forest Fragment (174), Pasture Land (148), Crop Fields (138), and Rural Settlement (136). The highest species-rich families were Geometridae (131 taxa), Erebidae (93 taxa), Tortricidae (40 taxa) and Sphingidae (17 taxa). Values calculated for the Shannon index were high, ranging between 4.78 for Natural Forests and 4.15 for Crop Fields. Simpson index indicated very high values of evenness, with all land use types above 0.97. The highest values of dominance were observed in Crop Field and Pasture Land, with the percentage of the dominant species around 10%. Estimated species richness by Chao-1 was highest within Natural Forest (366), followed by Pasture Land (256), Forest Fragment (241), Rural Settlement (233), and lowest in Crop Field (186), with the highest increase, if compared to the observed number of species, in Pasture Land (+ 73%) and Rural Settlement (+ 71%).
Conclusions:
The results of this study will be useful for guiding conservation management priorities for preventing irreversible biodiversity losses and maintaining ecosystem services provisioning that are essential conditions for the sustainable development of rural communities.
The effect of adding exogenous LH to IVF/ICSI cycles on pregnancy outcomes in elderly women with PCOS: a retrospective cohort study
Authors: Ju Wenhan , Lu Xiaoliu , Zhao Shuai , Song Jingyan , Wu Haicui , Guan Lu , Xiang Shan , Lian Fang
Published: December 2022

Background
Current findings on the necessity of adding exogenous luteinizing hormone (LH) to IVF/ICSI cycles in elderly women(≥ 35 years) with Polycystic ovary syndrome（PCOS）are controversial. This study investigated the clinical efficacy of adding exogenous LH to antagonist regimens in IVF/ICSI-assisted conception cycles for elderly patients with PCOS.
Methods
Clinical data of PCOS patients who underwent IVF/ICSI-assisted conception at Reproductive and Genetic Center, Affiliated Hospital of Shandong University of Traditional Chinese Medicine， between January 1, 2018, and April 30, 2022, were collected for retrospective cohort analysis. Groups 1 (exogenous follicle-stimulating hormone (FSH) only, n = 170) and 2 (exogenous FSH and LH, n = 265) were divided according to whether exogenous LH was added to the gonadotropin-releasing hormone antagonist (GnRH-ant) regimen. controlled ovarian hyperstimulation (COH) and conception outcomes were compared between the two groups， and binary logistic regression analysis was used to examine the association between the addition of exogenous LH and pregnancy outcome.
Results
The number of follicles ≥ 14 mm on trigger day（12.70±8.56 vs 11.08±6.84，P=0.025）, eggs obtained（13.28±9.02 vs 11.31±6.03，P=0.003）, fertilized eggs（10.79±7.87 vs 10.02±5.75，P=0.047）, normally fertilized eggs（8.07±6.47 vs 6.84±4.53，P=0.005）, and high-quality embryos（1.62±2.10 vs 1.01±1.41，P＜0.001）, and the cumulative clinical pregnancy rate（65.9% vs 49.1%，P=0.001）were significantly higher in group 1 than in group 2 (P < 0.05). The binary logistic regression indicated that adding exogenous LH was an independent risk factor for cumulative clinical pregnancy rate (OR = 2.024, P = 0.001).
Conclusion
For most elderly women with PCOS, particularly those with body mass index (BMI) < 25 kg/m2, the addition of exogenous LH during IVF/ICSI cycles resulted in decreased efficacy in assisted conception outcomes.
Effects of Corn oligopeptide on Dexamethasone-induced muscle atrophy in Sprague-Dawley rats
Authors: Wang Yunfeng , Yan Jiuming , Shi Lei , Zhou Zhengming , Guo Jiankui , Wei Yaoyao , Hu Wen
Published: December 2022

Corn oligopeptide (COP) derived from corn gluten meal (CGM) was proved to possess plenty of biological properties. However, there is no scientific evidence to support that COP can ameliorate muscle atrophy. To find a new therapeutic nutritional substrate of muscle atrophy, we investigated the anti-myopathy effects of COP in DEX-inducted rat muscle atrophy model and tried to explore the underlying mechanisms. Co-administration of COP (2.0g/kg.bw) with DEX for 5 weeks reversed DEX-induced reduction in gastrocnemius (Gas) and plantaris (Pla) muscles weight, grip strength, exercise tolerance and myofiber cross sectional area (CSA) of soleus (Sol), up-regulated the protein levels of Phospho-Akt and mTOR that are closely relate to protein synthesis, and down-regulated the expression of FoxO3a, NF-κBp65, Atrogin-1, MuRF-1, LC3 and ULK1 that are the key components of Ubiquitin–proteasome system and Autophagy that activate proteolysis. In addition, according to muscles weight, grip strength, exercise tolerance changing over experiment time, we found the three indexes were all improved at 3rd week. Therefore, we conclude that the optimal duration for COP to improve DEX-induced muscle atrophy might be 3 weeks, and mechanistically, COP activates protein synthesis through Akt/mTOR pathway, while inhibits proteolysis by inactivating the expression of Ubiquitin proteasome system (UPS) and Autophagy.
Risk Constellation Of Hospital Acquired Pressure Injuries In Patients With A Spinal Cord Injury/ Disorder - Focus On Time Since Spinal Cord Injury/ Disorder And Patients’ Age
Authors: Scheel-Sailer Anke , Neuhauser Charlotte , Sailer Clara , Najmanova Klara , Baumberger Michael , Paez-Granados Diego , Schaefer Dirk , Reto Wettstein
Published: December 2022

Study Design: Prospective cohort study.
Objectives: The aim of this study was to evaluate how time since spinal cord injury/disorder (SCI/D) and patients’ age influence risk constellation for hospital acquired pressure injuries (HAPI) in patients with a SCI/D.
Setting: Acute care and rehabilitation clinic specialized in SCI/D.
Methods: We collected patients’ characteristics and 85 risk factors for HAPI development in adults with SCI/D with at least one HAPI during their inpatient stay between August 2018 and December 2019. We analyzed patients’ characteristics and HAPI risk factors using descriptive statistics according to time since SCI/D (<1 year, 1-15 years, >15 years) and patients’ age (18-35 years, 35-65 years, >65 years).
Result: We identified 182 HAPI in 96 patients. Comparing patients with SCI/D <1 year with the other groups, autonomic dysreflexia (p<0.001), abnormal body temperature (p=0.001), hypertensive episode (p=0.005), and pneumonia (p<0.001) occurred more frequently; mean hemoglobin (p<0.001), albumin (p=0.002) and vitamin D levels (p=0.013) were significantly lower, and patients with time since SCI/D <1 year scored fewer points (10-12) on the Braden Scale (p<0.001). Comparing groups per patients’ age, only the SCIPUS score was higher in patients >65 years compared to the other two groups (p=0.002).
Conclusion: Different risk factor constellation seem to be underlying HAPI development with more differences in patients time since SCI/D than patients’ age. Awareness of these differences in risk factor constellation depending on time since SCI/D in these patients might lead to different HAPI prevention strategies.
Impact of hydrogen gas inhalation during therapeutic hypothermia on cerebral hemodynamics and oxygenation in the asphyxiated piglet
Authors: Nakamura Shinji , Nakao Yasuhiro , Yinmon Htun , Mitsuie Tsutomu , Koyano Kosuke , Morimoto Aya , Konishi Yukihiko , Arioka Makoto , Kondo Sonoko , Kato Ikuko , Ohta Kenichi , Yasuda Saneyuki , Miki Takanori , Ueno Masaki , Kusaka Takashi
Published: December 2022

We previously reported the neuroprotective potential of combined hydrogen (H2) gas ventilation therapy and therapeutic hypothermia (TH) by assessing the short-term neurological outcomes and histological findings of 5-day neonatal hypoxic-ischemic (HI) encephalopathy piglets. However, the effects of H2 gas on cerebral circulation and oxygen metabolism and on prognosis were unknown. Here, we used near-infrared time-resolved spectroscopy to compare between combined H2 gas ventilation and TH and TH alone. Piglets were divided into three groups: HI insult with normothermia (NT, n = 10), HI insult with hypothermia (TH, 33.5 ± 0.5°C, n = 8), and HI insult with hypothermia plus H2 ventilation (TH + H2, 2.1–2.7%, n = 8). H2 ventilation and TH were administered and the CBV and ScO2 were recorded for 24 h after the insult. CBV was significantly higher at 24 h after the insult in the TH + H2 group than in the other groups. ScO2 was significantly lower throughout the 24 h after the insult in the TH + H2 group than in the NT group. In conclusion, combined H2 gas ventilation and TH can increase CBV and decrease ScO2, which possibly reflects elevated cerebral blood flow to meet greater oxygen demand for more surviving neurons versus TH alone.
Application of pedicle axis view in percutaneous screw placement for type III fracture dislocation of the sacroiliac joint
Authors: Zhao Zhongzhen , Zheng Guofu , Chu Xiaodong , Wang Shuofan
Published: December 2022

Aim
To investigate the clinical application of pedicle axis view in percutaneous screw placement for type III fracture dislocation of the sacroiliac joint.
Methods
Percutaneous sacroiliac screw fixation was performed in 29 patients with type III fractures of the sacroiliac joint under imaging monitoring by pedicle axis view of the X-ray fluoroscopy (C-arm) after preoperative traction reduction and preoperative preparation. There were 19 males and 10 females, aged from 20 to 75 years old, with a mean age of 42.1 ± 3.4 years.
Results
The operation time was between 44 and 135 minutes, with a mean of 95.5 ± 9.4 minutes; the intraoperative fluoroscopy time was between 15 and 42 seconds, with a mean of 25 ± 4.7 seconds; the intraoperative blood loss was 5–10 ml, with a mean of 7.1 ± 1.3 ml; according to the Matta scoring system, there were 41 cases with excellent outcomes and 4 cases with good outcomes; based on the definition by Neo et al., pedicle screw positions were categorised into 4 grades: grade 0, 41 screws, grade I, 2 screws, grade II, 2 screws, grade III, 0 screws, and grade 0 and I screws were excellent screws, with an excellent rate of 95.6%; Majeed functional score showed excellent in 37 cases and good in 8 cases, with an excellent and good rate of 100%; 29 cases were followed up between 3 and18 months, with a mean of 7.1 ± 1.2 months. All patients achieved anatomical reduction, with accurate screw placement, no bone penetration, no neurovascular injury, and the fractures of patients healed.
Conclusion
Pedicle axis view using fluoroscopy is a convenient and quick fluoroscopy method in percutaneous screw placement for type III fracture dislocation of the sacroiliac joint, with a high success rate, good safety and short fluoroscopy time.
Viral co-infection of oncogenic Human papillomavirus with Epstein–Barr Virus, Human herpesvirus 8 and Herpes Simplex Virus type 2 in malignant cervical cancer
Authors: Sosse Soukayna Alaoui , Tadlaoui Kaoutar Anouar , Benhassou Mustapha , Elkarroumi Mohamed , Elmzibri Mohamed , Ennaji Moulay Mustapha
Published: December 2022

Cervical cancer is considered the fourth most common malignancy and the fifth fatal cancer in women, oncogenic Human papillomavirus are considered a primary cause of development of cervical cancer. It has also been suggested that viral coexistence may also accelerate the progression of cervical lesions to cervical cancer.
This study aims to study the coinfection of Epstein Bar, Herpesvirus 8 and Hepes simplex type 2 infections in women with cervical cancer with the presence of Human papillomavirus and their correlations with the clinicopathologic characteristics of patients. In this study, 73 samples that tested positive for Human papillomavirus in previous study were used for the detection of Epstein Bar, Herpesvirus 8 and Hepes simplex type 2 in tumor tissue using Polymerase Chain Reaction techniques, and the clinical relevance was analyzed statistically. Of the 73 samples (48%) were infected with Epstein Bar, (24.65%) infected with Herpesvirus 8 However, none of the cases were infected with Hepes simplex type 2. The frequency of co-infections was 16.43% of cases. No significant association was found between co-infection and other clinicopathologic features. Therefore, these results represent arguments in favor of the role of Epstein Bar and Herpesvirus 8 among Human papillomavirus positive cases, as potential cofactors in cervical carcinogenesis, which could lead us to develop new therapeutics and preventive vaccines.
Identifying the best PCR enzyme for library amplification in NGS
Authors: Quail Michael , Corton Craig , Uphill James , Keane Jacqueline , Gu Yong
Published: December 2022

Background PCR amplification is a necessary step in many next generation sequencing (NGS) library preparation methods[1] [2]. Whilst many PCR enzymes are developed to amplify single targets efficiently, accurately and with specificity, few are developed to meet the challenges imposed by NGS PCR, namely unbiased amplification of a wide range of different sizes and GC content. As a result PCR amplification during NGS library prep often results in bias toward GC neutral and smaller fragments. As NGS has matured, optimised NGS library prep kits and polymerase formulations have emerged and in this study we have tested a wide selection of available enzymes for both short read Illumina library preparation and long fragment amplification ahead of long-read sequencing. Results We tested over 20 different Hi-fidelity PCR enzymes/NGS amplification mixes on a range of Illumina library templates of varying GC content and composition, and find that both yield and genome coverage uniformity characteristics of the commercially available enzymes varied dramatically. Three enzymes Quantabio RepliQa Hifi Toughmix, Watchmaker Library Amplification Hot Start Master Mix (2X) “Equinox” and Takara Ex Premier were found to give a consistent performance, over all genomes, that mirrored closely that observed for PCR free datasets. We also test a range of enzymes for long read sequencing by amplifying size fractionated S. cerevisiae DNA of average size 21.6 and 13.4kb respectively. Conclusion The enzymes of choice for short read (Illumina) library fragment amplification are Quantabio RepliQa Hifi Toughmix, Watchmaker Library Amplification Hot Start Master Mix (2X) “Equinox” and Takara Ex Premier, with RepliQa also being the best performing enzyme from the enzymes tested for long fragment amplification prior to long read sequencing.
Comparative expression analysis of microRNAs targeting GRAS genes induced by osmotic stress in two contrasting wheat genotypes
Authors: Mishra Shefali , Chaudhary Reeti , Sharma Pradeep
Published: December 2022

Background
MicroRNAs (miRNAs) are important nonprotein-coding genes in plants which participate in in almost all biological processes during abiotic and biotic stresses. Understanding how plants respond to various environmental conditions requires the identification of stress-related miRNAs, and recent years there has been an increased interest in studying miRNA genes and miRNA gene expression. Drought is one of the common environmental stresses limiting plant growth and development. To understand the role of miRNAs in response to osmotic stress, stress-specific miRNAs and their GRAS gene targets were validated.
Results
In this study, we analysed comparative expression patterns of the ten stress-responsive miRNAs and targeting GRAS genes involved in osmotic stress adaptation in order to understand the regulation behaviour of abiotic stress and miRNAs in two contrasting wheat genotype C-306 (drought tolerant) and WL-711 (drought sensitive). Three miRNAs were found to be up-regulated in response to stress, whereas seven miRNAs were found to be down-regulated as a result of the study. In contrast to miRNA, it was also discovered that GRAS genes as their targets were up-regulated during osmotic stress. Furthermore, miR397 and its target GRAS53 were shown to be down-regulated at distinct time intervals while being unaffected by osmotic stress. Variation in the level of expression of studied miRNAs in the presence of target genes provides a likely explanation of miRNA-based abiotic stress regulation. A regulatory network of miRNA and their targets revealed that fourteen miRNA interact with 55 GRAS targets from various subfamilies that contribute in the plant growth and development.
Conclusions
These results provide evidence for temporal and variety-specific differential regulation of miRNAs and their targets in wheat in response to osmotic stress and could help in defining the potential roles of miRNAs in plant adaptation to osmotic stress in future.
Knowledge, attitude, and practice of oxygen administration among nurses working in University of Gondar Comprehensive Specialized Hospital, North Gondar, Northwest Ethiopia, 2022.
Authors: Abitew Kennean
Published: December 2022

The concentration of oxygen in ambient air is 21.00%. It is one of the World Health Organization's lists of essential medications (WHO). However, a high oxygen concentration may alter the lung and result in oxygen poisoning. Surfactant production is impacted by high oxygen concentrations, which also cause alveoli to collapse. To prevent these hazards, the critical care nurse must be able to monitor oxygen administration and gradually reduce supplementary oxygen. One of the issues associated with inadequate understanding, attitude, and practice about oxygen therapy are still delivering appropriate oxygen administration, despite the fact that it is controllable. To provide high-quality treatment, nurses' skills, attitudes, and practices are crucial since they can significantly contribute to preventing respiratory issues caused by a lack of oxygen while hospitalized. Therefore, the purpose of this study was to evaluate nurses' practices, knowledge, and attitudes toward the administration of oxygen in 2022 at the University of Gondar Comprehensive Specialized Hospital in Northwest Ethiopia.Methods: Institutional based cross-sectional study was conducted among nurses working at the University of Gondar comprehensive specialized hospital from May 1 to August 5/2022. A simple random sampling method was used to select study participants and data was collected by presenting self-administrated questionnaires. The data were entered into Epi data version 4.6 and exported into SPSS version 22. Finally, summary statistics were used to describe the study population.Result: A total of 244 respondents have participated with a response rate of 100%. Of the total respondents, 55.3% of participants had good knowledge, 56.1% of study participants had favorable attitudes and 62.7% of participants scored good practice.Conclusion and Recommendation: More than half of the nurses were found to have adequate knowledge, attitude, and practice about oxygen administration. However, it is considerable and not enough. To enhance the knowledge, attitude, and practice of the nurses on oxygen administration more than the current status, the implementation of a comprehensive, systematic, and continuous educational program was recommended.
Production of virus-like particles of porcine circovirus type 2 in baculovirus expression system and its application for antibody detection
Authors: Li Yanwei , Yu Pingping , Bao Yaxuan , Ren Yuwen , Zhao Shaowei , Zhang Xuexian
Published: December 2022

Backgroud
Porcine circovirus type 2 (PCV-2) infection is a growing and persistent threat to the swine industry, and thus the development of serological detection methods for PCV2 is of great necessity for clinical diagnosis, as well as epidemiological investigations. The study aimed to build an indirect enzyme-linked immunosorbent assay (ELISA) to examine antibodies against PCV2 based on virus-like particle (VLP).
Results
Through transmission electron microscopy (TEM), the VLPs were morphologically similar to authentic PCV-2 viruses. Purified VLPs can be detected in immunoblots with PCV-2 antisera, and a predominant protein of approximately 30 kDa was determined by Western blot. The VLPs were shown to have good immunogenicity in mice and stimulated a high level of PCV2-specific antibody titers. The indirect ELISA can detect PCV2 antibody responses in animals had a diagnostic sensitivity and specificity of 98.33% and 93.33% compared to immunofluorescence assay (IFA), respectively. The intra-assay and inter-assay coefficient variations (CVs) within a plate was <10%, and the CV of different ELISA plates was <15%, indicating good repeatability. There was no cross-reaction of this ELISA with antisera against other porcine viruses. A total of 170 serum samples collected from different pig farms in China were tested for anti-PCV2 antibodies, and 151 (88.9%) of the 170 samples were PCV2 antibody positive.
Conclusion
Our findings suggest that this ELISA assay was rapid, specific, and reproducible and can be used for large-scale serological investigations of PCV2 antibodies in pigs.
Polar Photospheric Magnetic Field Evolution and Global Flux Transport
Authors: Petrie Gordon
Published: December 2022

The Sun's polar magnetic fields are of paramount importance in structuring the heliosphere and in seeding the activity cycle, but they are difficult to observe from the ecliptic plane. However, they are formed by observable active-region magnetic flux decay and transport processes. We give an observational description of photospheric flux transport and polar field evolution using the $>40$~yr series (1974-2017) of full-disk line-of-sight photospheric magnetograms from the NSO Kitt Peak Vacuum Telescope (KPVT) 512-channel and Spectromagnetograph (SPMG) and the Synoptic Optical Long-term Investigation of the Sun (SOLIS) Vector Spectro-Magnetograph (VSM). An analytical technique introduced by \citet{Durrantetal04} is adapted to analyze time series of full-disk magnetograms to estimate global photospheric flux transport self-consistently. The major hemispheric flux changes, due to cancellation or transport of leading-polarity flux across the equator, occur around activity maximum each cycle when peak levels of flux emerge $\ge 10^{\circ}$ from the equator. This highlights the efficiency of flux cancellation and transport within each hemisphere, allowing peak levels of leading-polarity flux to reach the equator at this time, letting peak trailing-polarity flux surge poleward. Polar flux evolution follows the hemispheric flux closely each cycle, but with temporal variations dampened by lower-latitude flux cancellation. Cycle 24 was distinguished by large hemispheric flux fluctuations forming a three-part structure: (1) dominant northern activity and north polar flux decrease toward zero; (2) dominant southern activity peak, swift south polar field decrease, polarity reversal, and growth while the north pole was stalled; and (3) southern activity decline, north again dominant, north polar flux growth with reversed polarity. Recent global photospheric field evolution and polar field changes are described using Global Oscillations Network Group (GONG, 2006-present) and Solar Dynamics Observatory Helioseismic and Magnetic Imager (SDO/HMI) line-of-sight magnetograms.
Role of cerebro-uterine ratio in late fetal growth restriction and in hypertensive disorders of pregnancy: A prospective observational study
Authors: Tyagi Shakun , Agarwal Ishita , Naik Sunita , Mala YM
Published: December 2022

Objective: To evaluate the role Cerebro-uterine ratio (CUR) for prediction of adverse perinatal outcomes in pregnancies with late Fetal Growth Restriction (FGR) and Hypertensive Disorders of Pregnancy (HDP)
Study design: Fifty women with FGR, fifty with HDP and 100 normal pregnancies between 34-38 weeks’ gestation were evaluated at a tertiary care centre by Ultrasound Doppler, CPR and CUR were determined and correlated with perinatal outcome.
Results: UtA S/D and PI were significantly higher in cases as compared to controls in both groups (p<0.05). Only CUR was significantly lower in HDP cases compared to controls (p<0.001). CUR showed a higher influence on the occurrence of adverse perinatal outcomes in both groups compared to CPR.
Conclusion: Uterine Artery Doppler is an important predictor of uteroplacental insufficiency. CUR can be a better predictor of adverse perinatal outcomes as compared to CPR in pregnancies complicated by HDP with or without FGR.
An iterative method to solve the nonlinear matrix equation $X^p =A+\sum\limits_{i = 1}^m {M_i^*(B+X^{-1})^{-1}{M_i}} $
Authors: Yu Siting , Peng Jingjing , Li Rui , Tang Zengao , Peng Zhenyun
Published: December 2022

Note: Please see pdf for full title and abstract with equations.
Nonlinear matrix equations $X^p =A+\sum\limits_{i = 1}^m {M_i^*(B+X^{-1})^{-1}{M_i}} $ has extensive applications in control theory, ladder networks, dynamic programming and stochastic filtering. In this paper a fixed point acceleration iteration algorithm is proposed and, based on the basic characteristics of the Thompson distance, the convergence and error estimator of the proposed algorithm are proved. Numerical comparison experiments show that the proposed algorithm is feasible and effective.
Mathematics Subject Classification (2010): 15A24 , 65F30.
Zimmermanne Laband syndrome and infantile systemic hyalinosis: an enigma with two separate terms with overlapping features: case report and literature review
Authors: Owlia Fatemeh , Navabazam Alireza , Akhavan-Karbasi Mohammad-Hasan , Barjoei Mohammad Moein Derakhshan
Published: December 2022

Background Zimmermann-Laband Syndrome (ZLS) and infantile systemic hyalinosis (ISH) are extremely rare genetic disorders characterized by various spectrum manifestations. In spite of other case reports, this case has been reported by oral medicine specialists and oral and maxillofacial surgeons with overlapping features of both syndromes. Case presentation In this study, we reported a 2-year-old female patient with gingival growth and brought about embedded erupted teeth in both jaws. The new aspect of this case report attributed to multiple papulo-nodular skin lesions barely reported by a kind of literature. Surgical excision was performed under general anesthesia. At six months of follow-up after surgery, mastication, and breathing problems were significantly improved. Aesthetic in terms of gingival appearance was ameliorated. Conclusions Although both syndromes remain an enigma for specialists to date because of their ambiguous presentations, the timely diagnosis could be crucial for prognosis and prevention of further serious surcharge. Dentists could have an important role in the diagnosis of rare disorders.
The effects of opioid-free anesthesia with dexmedetomidine and esketamine on postoperative anesthetic-related complications for hip surgery in the elderly – a randomized controlled trial
Authors: Ye Qiuping , Hu Yang , Xing Qijing , Zhang Ye , Wu Yun
Published: December 2022

Background: The routine perioperative use of opioids has recently been questioned due to opioid-related side effects, which can be potentially harmful in geriatric patients. This study aimed to evaluate the effects of opioid-free anesthesia in geriatric patients undergoing hip surgery.Methods: A total of 121 patients, aged 60 years or older, undergoing elective hip surgery were randomized to receive either opioid-free anesthesia with dexmedetomidine and esketamine (OFA group) or balanced anesthesia with opioids (CON group). All patients received a preoperative fascia iliaca block and postoperative patient-controlled analgesia using tramadol. The primary outcome was the incidence of a composite of anesthetic-related complications (nausea and vomiting, hypoxemia, ileus, urinary retention and delirium) within 48 hours postoperatively. The hemodynamics, postoperative pain and quality of life were also assessed.Results: The incidence of composite adverse events was significantly reduced in the OFA group compared with the CON group (35.0% vs. 62.3%, estimated difference: 27.3%, 95% confidence interval: 10.2%–44.4%, P = 0.003). Notably, patients in the OFA group experienced less postoperative nausea and vomiting (P= 0.040), and hypoxemia (P= 0.025) compared with those in the CON group. However, the incidences of postoperative ileus, urinary retention and delirium were comparable between the two groups. Also, patients in the OFA group had less pain in motion at 24 h postoperatively, as well as less risks of intraoperative hypotension and bradycardia (P<0.05). No significant differences in the postoperative quality of life were observed between the two groups.Conclusions: Opioid-free anesthesia with dexmedetomidine and esketamine reduced postoperative anesthetic-related complications and provided improved hemodynamic stability in geriatric patients undergoing hip surgery.Trial registration: Chinese Clinical Trial Registry, retrospectively registered on 05/02/2022 (www.chictr.org.cn; ChiCTR2200056421)
Detection and attribution of changes in river flow and sediment loads in the Nile basin
Authors: Nkwasa Albert , Chawanda Celray James , Schlemm Annika , Ekolu Job , Frieler Katja , Griensven Ann van
Published: December 2022

The Nile basin is the second largest basin in Africa and one of the regions experiencing high climatic diversity with variability and deteriorating water resources. As climate change is affecting most of the hydroclimatic variables across the world, this study assesses whether historical changes in river flow and sediment loads in the Nile basin may be attributed to climatic change. An impact attribution approach is employed by forcing a process-based model with a set of observational and counterfactual climate data for 69 years (1951–2019), from the impact attribution setup of the Inter-Sectoral Impact Model Intercomparison Project (ISIMIP3a). The nonparametric Mann–Kendall test is used to identify trends while long-term mean annual river flow and sediment load simulation differences between a model setup with observational and counterfactual climate data are calculated to allow for quantification of the climate change attribution. Results show a reasonable evidence of a long-term historical increase in river flows and sediment loads of the upper part of the Nile basin, largely attributed to changes in climate. Contrary, there is less impact of climate change on the river flows and sediment loads in the Eastern and downstream parts of the Nile basin. These findings show spatial differences in the sensitivity of impacts of climate on river flows and sediment loads while highlighting the most impacted region in the basin.
WITHDRAWN: Refining Readability by Tackling Redundancy and Time through Modified Rule based Extractive Summarization Approach for Low Resource Indian Languages-Hindi andPunjabi
Authors: Sachdeva Jainy , Jhatta Nitika , Bathla Ashok Kumar
Published: December 2022

A rule based extractive text summarization system is proposed to obtain comprehensible summarized text of input Hindi and Punjabi text documents. The input document to be summarized can be any handwritten text or any text from internet saved in .rtf format. It works on Hindi and Punjabi documents and summarizes text of any length i.e. system summarizes text irrespective of size. The extensive rule based approach is applied consisting preprocessing and processing phases. Each phase consists of rules which are to be followed to get the desired concise summary. In the preprocessing phase, rules of Tokenization, Stop word removal and segmentation are applied whereas in processing phase rules such as Monetary values, Measurement Values, Special Symbols, Equations, Figure Number in sentences are applied. Also, other features such as frequently occurring words and maximum length word are added to make the system output comprehendible. The system is made user friendly by proposing a graphical user interface (GUI). The generated summary by the proposed system is compared with the summary of documents generated by human experts and is evaluated in terms of parameters such as Compression Ratio and Execution Time. Further, accuracy is calculated based on Precision, Recall and F-score. It has been observed that the compression ratio obtained lies in the range of 39–50% for 200, 300 and 400 words paragraphs. The execution time is quite less which do varies with the size of the paragraph entered as the summary size varies. In general, it has been observed that the execution time for 200 words paragraph takes 1519.147 milliseconds, for 300 words paragraph the time is 3022.825 milliseconds whereas for 400 words size paragraph, the time taken is 4023.123 milliseconds. The time is quite less as compared to human generated summary. The accuracy obtained in each case is above 95% which is high as compared to humans and other state of art methods. This shows that the system is robust enough and provides definitive results.
MicroRNA-mediated repression of endocannabinoid CB1 receptor expression contributes to simvastatin-induced skeletal muscle toxicity
Authors: Kalkan Hilal , Panza Elisabetta , Pagano Ester , Ercolano Giuseppe , Moriello Claudia , Piscitelli Fabiana , Capasso Raffaele , Marzo Vincenzo Di , Iannotti Fabio
Published: December 2022

Statins are the most prescribed lipid-lowering agents worldwide. Their use is generally safe, although muscular toxicity occurs in 1 in 10.000 patients. In this study, we explored the role of the endocannabinoid system (ECS) during muscle toxicity induced by simvastatin. In murine C2C12 myoblasts exposed to simvastatin (30 µM), we found that the levels of the endocannabinoids 2-AG and AEA as well the expression of specific miRNAs (mostly miR-152) targeting the endocannabinoid CB1 gene were increased. Rimonabant, a selective CB1 antagonist, exacerbated simvastatin-induced toxicity in myoblasts, while the opposite effect was observed with GAT211, a CB1-positive allosteric modulator. In antagomiR-152-transfected myoblasts, simvastatin toxicity was prevented along with the rescue of CB1 expression. Notably, similar alterations were found in skeletal muscles of C57BL/6J mice treated with simvastatin 20 mg Kg-1 and in primary human myoblasts. In sum, we identified the ECS as a novel mechanism participating in statin-induced myopathy.
SPAG5: the upstream protein of Wnt and the target of curcumin to inhibit hepatocellular carcinoma?
Authors: Li Huiyang , Qin Yanfei , Huang Yiran , Wang Jinquan , Ren Biqiong
Published: December 2022

Background:Curcumin inhibits hepatocellular carcinoma (HCC) related Wnt pathway has been proved and sperm associated antigen 5 (SPAG5) has also been reported to work through Wnt. But there was no report about curcumin inhibiting Wnt by SPAG5 in HCC.Methods:We first confirm the expression of SPAG5 in tissues and cell lines. Then observe the effect of SPAG5 and the regulation of curcumin on SPAG5 and Wnt pathway proteins. Subsequently, knockdown SPAG5 and overexpression of SPAG5, and the effect of curcumin on the expression of SPAG5 and the Wnt pathway protein was observed.Results:The results showed that SPAG5 was highly expressed in cancer tissues. Compared with normal hepatocytes, the expression of SPAG5 in hepatoma cell lines increased significantly at both mRNA and protein levels. Curcumin inhibits the expression of SPAG5 in hepatoma cell lines and inhibits migration and promotes apoptosis. Curcumin inhibits the expression of SPAG5 and Cyclin D1 in overexpression SPAG5 cell lines, but the inhibition of curcumin on Cyclin D1 was weakened after SPAG5 was knockdown.Conclusions:We can infer preliminarily that SPAG5 may be the upstream regulatory protein of Wnt, notably, curcumin may inhibit Wnt by inhibiting SPAG5.
Clinicopathological and molecular features of non-small cell lung cancer without TKI therapy that transforms into small-cell lung cancer
Authors: Gao Qiqi , Xia Mengling , Chen Zhen , Zhang Lixin , Zhou Jie , Wu Shengjun , Chen Xinhua , Zheng Yulong
Published: December 2022

Background
Some patients with NSCLC have been found to transformed to SCLC after treatment, but there is little research about it. This study aimed to determine the clinicopathological features and the molecular pathogenesis of NSCLC patients without TKI therapy that transforms into SCLC.
Methods
We investigated six patients with advanced NSCLC without TKI therapy that were transformed into SCLCs. Whole genome sequencing was applied for six tumor specimens (included NSCLC and SCLC specimens of each patient)acquired from three patients to detect genetic predictors for small-cell transformation. The clinicopathological features of six patients was collected and analyzed. Also, The literature was reviewed in detail to identify similar cases of NSCLC-to-SCLC transformation.
Results
The basic condition of patients was similar to those without transformation. Five patients (with squamous cell carcinoma) were male, and one (with adenocarcinoma) was male; their average age was 64.7 years. Thoracic imaging revealed solid tumours with unclear borders and lobulated texture with uneven densities in six patients. All five tumours were univocal, and they had an average size of 43 mm. (The image information of one patient was not obtained).The average trasformation time was 9.8 months. After SCLC transformation of three patients with WES, the mutation spectrum changed, C > T decreased and C > A increased. Compared with the initial NSCLC, the CNV burden of transformed SCLC decreased significantly. Clonal evolution analysis showed that there are links and also difference between the clones of initial NSCLC and transformed SCLC.
Conclusion
Secondary biopsy is very important for evaluating genetic and histological changes and selecting appropriate treatment after treatment resistance. This importance is not only for patients treated with tyrosine kinase inhibitors, but also for patients treated with chemotherapy and immunotherapy. The mutation rate of p53 in transformed SCLC was similar to de novo SCLC, however, the mutation rate of RB1 in transformed SCLC was lower to de novo SCLC.
Characteristic and potential of the horses origin species strain Enterococcus moraviensis EMo 1-1Nik
Authors: Lauková Andrea , Styková Eva , Focková Valentína , Maďar Marián
Published: December 2022

Nowadays, developed more precisious identification techniques have allowed to identify and validate newer enterococcal species. Among those species, also the species Enterococcus moraviensis was validated, the representat of which was firstly isolated from surface waters. However, in this study, characteristic and bacteriocin potential of E. moraviensis strain EMo 1-1Nik isolated from buccal mucosa of Slovak warm-blood horse breed has been studied. BLASTn analysis allotted EMo 1-1Nik strain to the species E. moraviensis with percentage identity BLASTn 16S rRNA sequence in the strain up to 100% (99.93% similarity with E. moraviensis NR113937.1 strain). The EMo 1-1Nik strain has been provided with GenBank accession number MW326085 (for nucleotide sequence SUB8683960 Seq1). EMo 1-1Nik strain is hemolysis-negative (γ -hemolysis), DNase negative and gelatinase negative; absent of virulence factor genes, low-grade biofilm-positive (0.133 ± 0.36), mostly susceptible to tested antibiotics. Moreover, 60% of EMo1-1Nik colonies were found as bacteriocin-producing against the principal indicator Enterococcus avium EA5. EMo1-1Nik concentrated substance pH 4.5 (CS) showed the inhibitory activity against EA5 strain (200 AU/mL); CSs with pH 6.3 and 7.3 reached inhibitory activity 100 AU/mL against EA5 strain. Oppositelly, EMo 1-1Nik was susceptible to Mundticin EM41/3 (800 AU/mL) produced by fecal horses strain E. mundtii EM41/3. In spite of the preliminary results, it has been shown bacteriocin potential of safe E. moraviensis EMo1-1Nik strain. The additional studies are in processing.