bluuebunny/crossref_metadata_2025 huggingface dataset:
Serum Markers of Refractoriness and Enteropathy-Associated T-Cell Lymphoma in Coeliac Disease
Authors: Lenti Marco Vincenzo , Aronico Nicola , Giuffrida Paolo , Antoci Valentina , Santacroce Giovanni , Vanoli Alessandro , Klersy Catherine , Corazza Gino Roberto , Di Sabatino Antonio
Published: May 2021

The persistence or recurrence of symptoms in patients with coeliac disease (CD), despite a gluten-free diet (GFD), must prompt further work-up for excluding refractory CD (RCD). The aim of this study was to assess the accuracy of serum markers in predicting refractoriness in CD patients. This study included 72 patients affected by CD followed-up at our center, namely 49 uncomplicated CD before and after GFD and 23 RCD. Serum levels of chromogranin A (CgA) and β2-microglobuline were measured at baseline and at follow-up (median time of 13 months) in each group of patients. Cut-off points for each marker were estimated to differentiate RCD from uncomplicated CD patients. Serum levels of CgA and β2-microglobuline were significantly higher in patients with RCD compared to uncomplicated CD (p < 0.001), both at baseline and at follow-up, with no significant difference between RCD type 1 and type 2. The estimated cut-off point for CgA was 90.2 ng/mL (sensitivity 83%, specificity 100%), while for β2-microglobuline it was 696 mcg/L (sensitivity 100%, specificity of 100%). To conclude, CgA and β2-microglobuline could be useful serological markers of refractoriness in CD, with the ability to discriminate those patients who should undergo upper gastrointestinal endoscopy for making a definite diagnosis.
Longitudinal Evaluation of PD-L1 Expression on Circulating Tumor Cells in Non-Small Cell Lung Cancer Patients Treated with Nivolumab
Authors: Ikeda Mio , Koh Yasuhiro , Teraoka Shunsuke , Sato Koichi , Oyanagi Jun , Hayata Atsushi , Tokudome Nahomi , Akamatsu Hiroaki , Ozawa Yuichi , Endo Katsuya , Higuchi Masayuki , Nakanishi Masanori , Ueda Hiroki , Yamamoto Nobuyuki
Published: May 2021

Although programmed death-ligand 1 (PD-L1) expression on tumor tissue is a validated predictive biomarker for a PD-1 pathway blockade in non-small cell lung cancer (NSCLC), longitudinal changes in its expression during treatment remains elusive. Circulating tumor cells (CTCs) are assumed to reflect the transition of characteristics of the primary tumor undergoing anticancer treatment. Here, we sequentially evaluated the PD-L1 expression on CTCs in NSCLC patients treated with nivolumab. Forty-five patients were enrolled, and CTCs were enriched from 3 mL of peripheral blood using a microcavity array system at baseline and weeks 4, 8, 12, and 24 or until progressive disease. The effective responses to therapy were compared between patients without progressive disease (PD) at week 8 (i.e., non-PD patients) and in those with PD between weeks 4 and 8 (PD patients) in terms of increased vs. decreased or equal CTC status at week 8 (for non-PD patients) or at the point of PD (for PD patients) compared to the baseline. Significantly more non-PD patients were classified as decreased or equal in number and proportion to PD-L1-positive CTCs among the detected CTCs (PD-L1 positivity rates) (p < 0.05). Moreover, progression-free survival was significantly longer in patients with ≥7.7% PD-L1 positivity rates (n = 8) than in those with <7.7% rates (n = 8; p < 0.01) at week 8. These results suggest the predictive significance of the early evaluation of PD-L1 expression on CTCs for maintaining the benefits from nivolumab treatment.
Recent Advances in Implantation-Based Genetic Modeling of Biliary Carcinogenesis in Mice
Authors: Izumiya Masashi , Kato Shingo , Hippo Yoshitaka
Published: May 2021

Epithelial cells in the biliary system can develop refractory types of cancers, which are often associated with inflammation caused by viruses, parasites, stones, and chemicals. Genomic studies have revealed recurrent genetic changes and deregulated signaling pathways in biliary tract cancer (BTC). The causal roles have been at least partly clarified using various genetically engineered mice. Technical advances in Cre-LoxP technology, together with hydrodynamic tail injection, CRISPR/Cas9 technology, in vivo electroporation, and organoid culture have enabled more precise modeling of BTC. Organoid-based genetic modeling, combined with implantation in mice, has recently drawn attention as a means to accelerate the development of BTC models. Although each model may not perfectly mimic the disease, they can complement one another, or two different approaches can be integrated to establish a novel model. In addition, a comparison of the outcomes among these models with the same genotype provides mechanistic insights into the interplay between genetic alterations and the microenvironment in the pathogenesis of BTCs. Here, we review the current status of genetic models of BTCs in mice to provide information that facilitates the wise selection of models and to inform the future development of ideal disease models.
Expression of NK Cell Receptor Ligands on Leukemic Cells Is Associated with the Outcome of Childhood Acute Leukemia
Authors: Martínez-Sánchez María Victoria , Fuster José Luis , Campillo José Antonio , Galera Ana María , Bermúdez-Cortés Mar , Llinares María Esther , Ramos-Elbal Eduardo , Pascual-Gázquez Juan Francisco , Fita Ana María , Martínez-Banaclocha Helios , Galián José Antonio , Gimeno Lourdes , Muro Manuel , Minguela Alfredo
Published: May 2021

Acute leukemia is the most common malignancy in children. Most patients are cured, but refractory/relapsed AML and ALL are the first cause of death from malignancy in children. Maintenance chemotherapy in ALL has improved survival by inducing leukemic cell apoptosis, but immune surveillance effectors such as NK cells might also contribute. The outcome of B-ALL (n = 70), T-ALL (n = 16), and AML (n = 16) pediatric patients was evaluated according to leukemic cell expression of ligands for activating and inhibiting receptors that regulate NK cell functioning. Increased expression of ULBP-1, a ligand for NKG2D, but not that of CD112 or CD155, ligands for DNAM-1, was associated with poorer 5-year event-free survival (5y-EFS, 77.6% vs. 94.9%, p < 0.03). Reduced expression of HLA-C on leukemic cells in patients with the KIR2DL1/HLA-C*04 interaction was associated with a higher rate of relapse (17.6% vs. 4.4%, p = 0.035) and lower 5y-EFS (70.6% vs. 92.6%, p < 0.002). KIR2DL1/HLA-C*04 interaction was an independent predictive factor of events (HR = 4.795, p < 0.005) or death (HR = 6.731, p < 0.005) and might provide additional information to the current risk stratification. Children who carry the KIR2DL1/HLA-C*04 interaction were refractory to current chemotherapy treatments, including allogeneic stem cell transplantation; therefore, they should be considered as candidates for alternative biological therapies that might offer better results.
Diet-Induced Obesity Impairs Outcomes and Induces Multi-Factorial Deficiencies in Effector T Cell Responses Following Anti-CTLA-4 Combinatorial Immunotherapy in Renal Tumor-Bearing Mice
Authors: Turbitt William J. , Boi Shannon K. , Gibson Justin T. , Orlandella Rachael M. , Norian Lyse A.
Published: May 2021

Associations between modifiable factors and the efficacy of cancer immunotherapies remain uncertain. We found previously that diet-induced obesity (DIO) reduces the efficacy of an immunotherapy consisting of adenovirus-encoded TRAIL plus CpG oligonucleotide (AdT/CpG) in mice with renal tumors. To eliminate confounding effects of diet and determine whether outcomes could be improved in DIO mice, we evaluated AdT/CpG combined with anti-CTLA-4 in diet-matched, obese-resistant (OB-RES) versus DIO tumor-bearing mice. Therapy-treated OB-RES mice displayed effective renal tumor control and sustained CD4+ and CD8+ T cell responses. In contrast, therapy-treated DIO mice exhibited progressive tumor outgrowth and blunted T cell responses, characterized by reduced intratumoral frequencies of IFNγ+ CD4+ and CD8+ T cells. Weak effector T cell responses in therapy-treated DIO mice were accompanied by low intratumoral concentrations of the T cell chemoattractant CCL5, heightened concentrations of pro-tumorigenic GM-CSF, and impaired proliferative capacity of CD44+CD8+ T cells in tumor-draining lymph nodes. Our findings demonstrate that in lean mice with renal tumors, combining in situ T cell priming upstream of anti-CTLA-4 enhances outcomes versus anti-CTLA-4 alone. However, host obesity is associated with heightened immunotherapy resistance, characterized by multi-factorial deficiencies in effector CD4+ and CD8+ T cell responses that extend beyond the tumor microenvironment.
The Role of p53 Dysfunction in Colorectal Cancer and Its Implication for Therapy
Authors: Michel Maurice , Kaps Leonard , Maderer Annett , Galle Peter R. , Moehler Markus
Published: May 2021

Colorectal cancer (CRC) is one of the most common and fatal cancers worldwide. The carcinogenesis of CRC is based on a stepwise accumulation of mutations, leading either to an activation of oncogenes or a deactivation of suppressor genes. The loss of genetic stability triggers activation of proto-oncogenes (e.g., KRAS) and inactivation of tumor suppression genes, namely TP53 and APC, which together drive the transition from adenoma to adenocarcinoma. On the one hand, p53 mutations confer resistance to classical chemotherapy but, on the other hand, they open the door for immunotherapy, as p53-mutated tumors are rich in neoantigens. Aberrant function of the TP53 gene product, p53, also affects stromal and non-stromal cells in the tumor microenvironment. Cancer-associated fibroblasts together with other immunosuppressive cells become valuable assets for the tumor by p53-mediated tumor signaling. In this review, we address the manifold implications of p53 mutations in CRC regarding therapy, treatment response and personalized medicine.
Targeting the NLRP3 Inflammasome as a New Therapeutic Option for Overcoming Cancer
Authors: Missiroli Sonia , Perrone Mariasole , Boncompagni Caterina , Borghi Chiara , Campagnaro Alberto , Marchetti Francesco , Anania Gabriele , Greco Pantaleo , Fiorica Francesco , Pinton Paolo , Giorgi Carlotta
Published: May 2021

Inflammasomes are multiprotein complexes that regulate the maturation and secretion of the proinflammatory cytokines interleukin-1beta (IL-1β and interleukin-18 (IL-18) in response to various intracellular stimuli. As a member of the inflammasomes family, NLRP3 is the most studied and best characterized inflammasome and has been shown to be involved in several pathologies. Recent findings have made it increasingly apparent that the NLRP3 inflammasome may also play a central role in tumorigenesis, and it has attracted attention as a potential anticancer therapy target. In this review, we discuss the role of NLRP3 in the development and progression of cancer, offering a detailed summary of NLRP3 inflammasome activation (and inhibition) in the pathogenesis of various forms of cancer. Moreover, we focus on the therapeutic potential of targeting NLRP3 for cancer therapy, emphasizing how understanding NLRP3 inflammasome-dependent cancer mechanisms might guide the development of new drugs that target the inflammatory response of tumor-associated cells.
Plasma Protein Biomarkers Associated with Higher Ovarian Cancer Risk in BRCA1/2 Carriers
Authors: Ahn Hee-Sung , Ho Jung Yoon , Yu Jiyoung , Yeom Jeonghun , Lee Sanha , Hur Soo Young , Jung Yuyeon , Kim Kyunggon , Choi Youn Jin
Published: May 2021

Ovarian cancer (OC) is the most lethal gynecologic malignancy and in-time diagnosis is limited because of the absence of effective biomarkers. Germline BRCA1/2 genetic alterations are risk factors for hereditary OC; risk-reducing salpingo-oophorectomy (RRSO) is pursued for disease prevention. However, not all healthy carriers develop the disease. Therefore, identifying predictive markers in the BRCA1/2 carrier population could help improve the identification of candidates for preventive RRSO. In this study, plasma samples from 20 OC patients (10 patients with BRCA1/2 wild type (wt) and 10 with the BRCA1/2 variant (var)) and 20 normal subjects (10 subjects with BRCA1/2wt and 10 with BRCA1/2var) were analyzed for potential biomarkers of hereditary OC. We applied a bottom-up proteomics approach, using nano-flow LC-MS to analyze depleted plasma proteome quantitatively, and potential plasma protein markers specific to the BRCA1/2 variant were identified from a comparative statistical analysis of the four groups. We obtained 1505 protein candidates from the 40 subjects, and SPARC and THBS1 were verified by enzyme-linked immunosorbent assay. Plasma SPARC and THBS1 concentrations in healthy BRCA1/2 carriers were found to be lower than in OC patients with BRCA1/2var. If plasma SPARC concentrations increase over 337.35 ng/mL or plasma THBS1 concentrations increase over 65.28 μg/mL in a healthy BRCA1/2 carrier, oophorectomy may be suggested.
Berberine Improves Cognitive Impairment by Simultaneously Impacting Cerebral Blood Flow and β-Amyloid Accumulation in an APP/tau/PS1 Mouse Model of Alzheimer’s Disease
Authors: Ye Chenghui , Liang Yubin , Chen Ying , Xiong Yu , She Yingfang , Zhong Xiaochun , Chen Hongda , Huang Min
Published: May 2021

Alzheimer’s disease (AD) is accompanied by β-amyloid (Aβ), neurofibrillary tangles, and neuron cell death, and is one of the most commonly occurring diseases among the elderly. The pathology of AD is complex, involving Aβ overproduction and accumulation, tau hyperphosphorylation, and neuronal loss. In addition, chronic cerebral hypoperfusion (CCH) is ubiquitous in the AD patients and plans a pivotal role in triggering and exacerbating the pathophysiological progress of AD. The goal of this study was to investigate the neuroprotective properties of berberine (BBR) and the underlying mechanism. During the study, BBR was administrated to treat the triple-transgenic mouse model of Alzheimer’s disease (3×Tg AD). To thoroughly evaluate the effects of the BBR administration, multiple manners were utilized, for instance, 3D arterial spin labeling technique, Morris water maze assay, immunofluorescence staining, TUNEL assay, laser speckle contrast imaging, western blotting, etc. The results showed that BBR ameliorated cognitive deficits in 3×Tg AD mice, reduced the Aβ accumulation, inhibited the apoptosis of neurons, promoted the formation of microvessels in the mouse brain by enhancing brain CD31, VEGF, N-cadherin, Ang-1. The new vessels promoted by BBR were observed to have a complete structure and perfect function, which in turn promoted the recovery of cerebral blood flow (CBF). In general, berberine is effective to 3×Tg AD mice, has a neuroprotective effect, and is a candidate drug for the multi-target prevention and treatment of AD.
Anthracyclins Increase PUFAs: Potential Implications in ER Stress and Cell Death
Authors: Balgoma David , Kullenberg Fredrik , Calitz Carlemi , Kopsida Maria , Heindryckx Femke , Lennernäs Hans , Hedeland Mikael
Published: May 2021

Metabolic and personalized interventions in cancer treatment require a better understanding of the relationship between the induction of cell death and metabolism. Consequently, we treated three primary liver cancer cell lines with two anthracyclins (doxorubicin and idarubin) and studied the changes in the lipidome. We found that both anthracyclins in the three cell lines increased the levels of polyunsaturated fatty acids (PUFAs) and alkylacylglycerophosphoethanolamines (etherPEs) with PUFAs. As PUFAs and alkylacylglycerophospholipids with PUFAs are fundamental in lipid peroxidation during ferroptotic cell death, our results suggest supplementation with PUFAs and/or etherPEs with PUFAs as a potential general adjuvant of anthracyclins. In contrast, neither the markers of de novo lipogenesis nor cholesterol lipids presented the same trend in all cell lines and treatments. In agreement with previous research, this suggests that modulation of the metabolism of cholesterol could be considered a specific adjuvant of anthracyclins depending on the type of tumor and the individual. Finally, in agreement with previous research, we found a relationship across the different cell types between: (i) the change in endoplasmic reticulum (ER) stress, and (ii) the imbalance between PUFAs and cholesterol and saturated lipids. In the light of previous research, this imbalance partially explains the sensitivity to anthracyclins of the different cells. In conclusion, our results suggest that the modulation of different lipid metabolic pathways may be considered for generalized and personalized metabochemotherapies.
Small-RNA Sequencing Reveals Altered Skeletal Muscle microRNAs and snoRNAs Signatures in Weanling Male Offspring from Mouse Dams Fed a Low Protein Diet during Lactation
Authors: Kanakis Ioannis , Alameddine Moussira , Folkes Leighton , Moxon Simon , Myrtziou Ioanna , Ozanne Susan E. , Peffers Mandy J. , Goljanek-Whysall Katarzyna , Vasilaki Aphrodite
Published: May 2021

Maternal diet during gestation and lactation affects the development of skeletal muscles in offspring and determines muscle health in later life. In this paper, we describe the association between maternal low protein diet-induced changes in offspring skeletal muscle and the differential expression (DE) of small non-coding RNAs (sncRNAs). We used a mouse model of maternal protein restriction, where dams were fed either a normal (N, 20%) or a low protein (L, 8%) diet during gestation and newborns were cross-fostered to N or L lactating dams, resulting in the generation of NN, NL and LN offspring groups. Total body and tibialis anterior (TA) weights were decreased in weanling NL male offspring but were not different in the LN group, as compared to NN. However, histological evaluation of TA muscle revealed reduced muscle fibre size in both groups at weaning. Small RNA-sequencing demonstrated DE of multiple miRs, snoRNAs and snRNAs. Bioinformatic analyses of miRs-15a, -34a, -122 and -199a, in combination with known myomiRs, confirmed their implication in key muscle-specific biological processes. This is the first comprehensive report for the DE of sncRNAs in nutrition-associated programming of skeletal muscle development, highlighting the need for further research to unravel the detailed molecular mechanisms.
Sex Hormone Receptor Signaling in Bladder Cancer: A Potential Target for Enhancing the Efficacy of Conventional Non-Surgical Therapy
Authors: Ide Hiroki , Miyamoto Hiroshi
Published: May 2021

There have been critical problems in the non-surgical treatment for bladder cancer, especially residence to intravesical pharmacotherapy, including BCG immunotherapy, cisplatin-based chemotherapy, and radiotherapy. Recent preclinical and clinical evidence has suggested a vital role of sex steroid hormone-mediated signaling in the progression of urothelial cancer. Moreover, activation of the androgen receptor and estrogen receptor pathways has been implicated in modulating sensitivity to conventional non-surgical therapy for bladder cancer. This may indicate the possibility of anti-androgenic and anti-estrogenic drugs, apart from their direct anti-tumor activity, to function as sensitizers of such conventional treatment. This article summarizes available data suggesting the involvement of sex hormone receptors, such as androgen receptor, estrogen receptor-α, and estrogen receptor-β, in the progression of urothelial cancer, focusing on their modulation for the efficacy of conventional therapy, and discusses their potential of overcoming therapeutic resistance.
The Amine Group as Halogen Bond Acceptor in Cocrystals of Aromatic Diamines and Perfluorinated Iodobenzenes
Authors: Uran Erik , Fotović Luka , Bedeković Nikola , Stilinović Vladimir , Cinčić Dominik
Published: May 2021

In order to study the proclivity of primary amine groups to act as halogen bond acceptors, three aromatic diamines (p-phenylenediamine (pphda), benzidine (bnzd) and o-tolidine (otol)) were cocrystallised with three perfluorinated iodobenzenes (1,4-tetrafluorodiiodobenzene (14tfib), 1,3-tetrafluorodiiodobenzene (13tfib) and 1,3,5-trifluorotriiodobenzene (135tfib)) as halogen bond donors. Five cocrystals were obtained: (pphda)(14tfib), (bnzd)(13tfib)2, (bnzd)(135tfib)4, (otol)(14tfib) and (otol)(135tfib)2. In spite of the variability of both stoichiometries and structures of the cocrystals, in all the prepared cocrystals the amine groups form exclusively I···N halogen bonds, while the amine hydrogen atoms participate mostly in N–H⋯F contacts. The preference of the amine nitrogen atom toward the halogen bond, as opposed to the hydrogen bond (with amine as a donor), is rationalised by means of computed hydrogen and halogen bond energies, indicating that the halogen bond energy between a simple primary amine (methylamine) and a perfluorinated iodobenzene (pentafluoroiodobenze ne) is ca. 15 kJ mol−1 higher than the energy of the (H)NH∙∙∙NH2 hydrogen bond between two amine molecules.
Helium Effects on the Mechanical Properties of Nanocrystalline Fe: Based on Molecular Dynamics
Authors: Xu Chunping , Yang Dongyan
Published: May 2021

A molecular dynamics (MD) simulation study was performed to investigate the effects of helium (He) on the mechanical properties of nanocrystalline body-centered cubic iron (BCC Fe). Simulated X-ray diffraction (XRD) was used to explore the relationship between the generation of cracks and the change of the crystal structure in nanocrystalline BCC Fe during tensile deformation. It is observed that the peak stress and the elastic modulus decrease with increasing concentration of He atoms, which are introduced into the grain boundary (GB) region of nanocrystalline Fe. The generation and connection of intergranular cracks are enhanced by He atoms. Significant peak separation, which is associated with the generation of cracks, is found in the simulated XRD patterns of nanocrystalline Fe during the tensile process. The lower diffraction angle of the {211}′ peak suggests a more serious lattice distortion during loading. For all nanocrystalline Fe deformed to 6% strain, the degree and fraction of the lattice distortion increases with the increasing loading stress.
Pulse Coupled Neural Network-Based Multimodal Medical Image Fusion via Guided Filtering and WSEML in NSCT Domain
Authors: Li Liangliang , Ma Hongbing
Published: May 2021

Multimodal medical image fusion aims to fuse images with complementary multisource information. In this paper, we propose a novel multimodal medical image fusion method using pulse coupled neural network (PCNN) and a weighted sum of eight-neighborhood-based modified Laplacian (WSEML) integrating guided image filtering (GIF) in non-subsampled contourlet transform (NSCT) domain. Firstly, the source images are decomposed by NSCT, several low- and high-frequency sub-bands are generated. Secondly, the PCNN-based fusion rule is used to process the low-frequency components, and the GIF-WSEML fusion model is used to process the high-frequency components. Finally, the fused image is obtained by integrating the fused low- and high-frequency sub-bands. The experimental results demonstrate that the proposed method can achieve better performance in terms of multimodal medical image fusion. The proposed algorithm also has obvious advantages in objective evaluation indexes VIFF, QW, API, SD, EN and time consumption.
An Analog Voltage Similarity Circuit with a Bell-Shaped Power Consumption
Authors: Azadmehr Mehdi , Marchetti Luca , Berg Yngvar
Published: May 2021

This paper presents a voltage similarity circuit (bump circuit) based on a novel voltage correlator. The proposed circuit is characterized by a power consumption which depends on the similarity between the two inputs. The sensitivity of the bump circuit and the power consumption are at the highest values when the inputs are equal. As the similarity between the input voltages decreases, the total current consumption decreases with a bell-shaped behavior. The proposed bump circuit is very simple in design, made of a new voltage correlator circuit in combination with a differential pair and mimics the behavior of the classical bump circuit. The voltage correlator was implemented using AMS−350nm CMOS technology. Simulation and measurement results suggests that a low power consumption may be achieved if the circuit is used in applications where the input signals have large dissimilarity for most of the circuit operation.
A Short-Term Residential Load Forecasting Model Based on LSTM Recurrent Neural Network Considering Weather Features
Authors: Wang Yizhen , Zhang Ningqing , Chen Xiong
Published: May 2021

With economic growth, the demand for power systems is increasingly large. Short-term load forecasting (STLF) becomes an indispensable factor to enhance the application of a smart grid (SG). Other than forecasting aggregated residential loads in a large scale, it is still an urgent problem to improve the accuracy of power load forecasting for individual energy users due to high volatility and uncertainty. However, as an important variable that affects the power consumption pattern, the influence of weather factors on residential load prediction is rarely studied. In this paper, we review the related research of power load forecasting and introduce a short-term residential load forecasting model based on a long short-term memory (LSTM) recurrent neural network with weather features as an input.
Turkmenistan’s Gas Sector Development Scenarios Based on Econometric and SWOT Analysis
Authors: Iwaszczuk Natalia , Wolak Jacek , Iwaszczuk Aleksander
Published: May 2021

Turkmenistan is currently one of the key suppliers in the global natural gas market, and it is important to examine its competitive position in the world. The aim of this article is to forecast the development strategy of its gas sector. To achieve this aim, the authors first examined: (1) Turkmenistan’s position in the global gas market and the structure of its oil and gas sector; (2) the share of gas in Turkmenistan’s exports. They then conducted an analysis of the causal relationship between FDI and GDP. The above research made it possible to conduct a comprehensive SWOT analysis. Based on the SWOT analysis, three sector development scenarios were developed: pessimistic, realistic, and optimistic. On their basis, the gas sector in Turkmenistan can build strategies for its development, considering the impact of market and non-market factors.
Field Study on the Thermal Performance of Vacuum Tube Solar Collectors in the Climate Conditions of Western Norway
Authors: Popsueva Victoria , Lopez Andrés Franklin Olivares , Kosinska Anna , Nikolaev Oleg , Balakin Boris V.
Published: May 2021

A significant part of energy consumption in Northern countries goes to heating. There is no consensus about the most efficient source of renewable heat there. This paper presents a field study for a 7.8 m2 vacuum tube solar collector facility that is conservatively located in the cloudy and cold climate conditions of western Norway. We analyse a year-long operation by examining the rig’s statistics. We show that in Nordic latitudes with rainy climate conditions, a domestic solar hot water system can produce 2200 kWhth/y at a thermal efficiency of up to 72%. The average amount of heat produced by the collectors was up to 14.7 kWhth/d. This was enough to sustain the domestic hot water demand in an average Norwegian household for 6 months with a short period of auxiliary heating. In conclusion, we calculated that a 3× upscaled area facility would deliver over 25 kWhth covering six months of total heat consumption. The payback period for the facility is 12 years.
Increased Internal Combustion Engine Efficiency with Optimized Valve Timings in Extended Stroke Operation
Authors: Omanovic Andyn , Zsiga Norbert , Soltic Patrik , Onder Christopher
Published: May 2021

Spark-ignited internal combustion engines are known to exhibit a decreased brake efficiency in part-load operation. Similarly to cylinder deactivation, the x-stroke operation presented in this paper is an adjustable form of skip-cycle operation. It is an effective measure to increase the efficiency of an internal combustion engine, which has to be equipped with a variable valve train to enable this feature. This paper presents an optimization procedure for the exhaust valve timings applicable to any valid stroke operation number greater than four. In the first part, the gas spring operation, during which all gas exchange valves are closed, is explained, as well as how it affects the indicated efficiency and the blow-by mass flow. In the second part, a simulation model with variable valve timings, parameterized with measurement data obtained on the engine test, is used to find the optimal valve timings. We show that in 12-stroke operation and with a cylinder load of 5 Nm, an indicated efficiency of 34.3% is achieved. Preloading the gas spring with residual gas prevents oil suction and thus helps to reduce hydrocarbon emissions. Measurements of load variations in 4-, 8-, and 12-stroke operations show that by applying an x-stroke operation, the indicated efficiency remains high and the center of combustion remains optimal in the range of significantly lower torque outputs.
COVID-19 and the Environment, Review and Analysis
Authors: Lipfert Frederick W. , Wyzga Ronald E.
Published: May 2021

We reviewed studies linking COVID-19 cases and deaths with the environment, focusing on relationships with air pollution. We found both short- and long-term observational relationships with a range of regulated pollutants, although only two studies considered both cases (i.e., infections) and deaths within a common analytical framework. Most of these studies were limited to a few months of the pandemic period. Statistically significant relationships were found more often for PM2.5 and NO2 than for other regulated pollutants, but no rationale was suggested for such short-term relationships; latency was seldom considered for long-term relationships. It was also unclear whether confounding had been adequately controlled in either type of study. Studies of air quality improvement following lockdowns found more robust relationships with local (CO, NO2) rather than regional (PM2.5, O3) pollutants, but meteorological confounding was seldom considered. Only one of seven studies of airborne virus transmission reported actual measurements. Overall, we found the existing body of literature to be more suggestive than definitive. Due to these various deficiencies, we assembled a new state-level database of cumulative COVID-19 cases and deaths through March 2021 with a range of potential predictor variables and performed linear regression analyses on various combinations. As single predictors, we found significant (p < 0.05) relationships between cumulative cases and household crowding (+), education (−), face-mask usage (−), or voting Republican (+). For cumulative deaths, we found significant relationships with education (−), black race (+), or previous levels of PM2.5 (+). NOx (+), and elemental carbon (EC, +). We found no relationships between long-term air quality and cumulative COVID-19 cases. Our associations linking air pollution with COVID-19 mortality were not statistically different from those for all-cause mortality in previous studies. In multiple mortality regressions combining air pollution, race, and education, NOx and EC remained significant but PM2.5 did not. We concluded that the current worldwide emphasis on PM2.5 is misplaced. We predicted air pollutant effects of a few percentage points, but individual differences between races, political identification, and post-graduate education were of the order of factors of 2 to 4. In general, the factors predicting infection were personal and related to COVID-19 exposure, while those predicting subsequent mortality tended to be more situational and related to geography. Overall, we concluded that how you live is more important than where you live.
Comparative Efficacy Trials with Two Different Bacillus thuringiensis Serovar kurstaki Strains against Gypsy Moth in Mediterranean Cork Oak Forests
Authors: Olivieri Maurizio , Mannu Roberto , Ruiu Luca , Ruiu Pino A. , Lentini Andrea
Published: May 2021

The efficacy of two formulations (Foray® 76B AVIO and Rapax® AS AIR) containing different Bacillus thuringiensis kurstaki (Btk) strains (ABTS-351 and EG-2348, respectively) was evaluated against Lymantria dispar larval populations in cork oak forests in Sardinia (Italy), in 2018 and 2019. The experimental design involved the following treatments: (I) untreated control; (II) Foray® 76B at the dose of 2.0 L/ha; (III) Foray® 76B at the dose of 2.5 L/ha; (IV) Rapax® AS AIR at the dose of 2.0 L/ha. Aerial applications were carried out using a helicopter equipped with four electronic rotary atomizers adjusted to sprinkle 160 micron-sized drops. Btk efficacy was evaluated by assessing the larval density reduction 7, 14, and 21 days after the application in each experimental plot in comparison with an untreated check. In addition to field surveys, the mortality of second and third instar larval samples, randomly collected from each plot after treatment and fed with foliage from the same plot, was determined in the laboratory. All Btk treatments were similarly effective, and no differences in larval density reduction among Btk strains and doses were found in either year. Twenty-one days after application, the average larval density reduction in the field was approximately 70% in all treated plots in 2018, whereas in 2019 it reached 80% only in areas treated with Foray 76B at 2.5 L/ha. Laboratory observations showed that all Btk-based products were effective against gypsy moth larvae, with significant differences in mortality between untreated control and the different Btk treatments. Our results shed light on the possibility of alternating different Btk strains for resistance management purposes and of applying lower doses than labeled, in order to achieve cost savings for product shipment and distribution and to reduce the environmental impact.
A Novel Phenotype of Junctional Epidermolysis Bullosa with Transient Skin Fragility and Predominant Ocular Involvement Responsive to Human Amniotic Membrane Eyedrops
Authors: Castiglia Daniele , Fortugno Paola , Condorelli Angelo Giuseppe , Barresi Sabina , De Luca Naomi , Pizzi Simone , Neri Iria , Graziano Claudio , Trojan Diletta , Ponzin Diego , Rossi Sabrina , Zambruno Giovanna , Tartaglia Marco
Published: May 2021

Junctional epidermolysis bullosa (JEB) is a clinically and genetically heterogeneous skin fragility disorder frequently caused by mutations in genes encoding the epithelial laminin isoform, laminin-332. JEB patients also present mucosal involvement, including painful corneal lesions. Recurrent corneal abrasions may lead to corneal opacities and visual impairment. Current treatments are merely supportive. We report a novel JEB phenotype distinguished by the complete resolution of skin fragility in infancy and persistent ocular involvement with unremitting and painful corneal abrasions. Biallelic LAMB3 mutations c.3052-5C>G and c.3492_3493delCG were identified as the molecular basis for this phenotype, with one mutation being a hypomorphic splice variant that allows residual wild-type laminin-332 production. The reduced laminin-332 level was associated with impaired keratinocyte adhesion. Then, we also investigated the therapeutic power of a human amniotic membrane (AM) eyedrop preparation for corneal lesions. AM were isolated from placenta donors, according to a procedure preserving the AM biological characteristics as a tissue, and confirmed to contain laminin-332. We found that AM eyedrop preparation could restore keratinocyte adhesion in an in vitro assay. Of note, AM eyedrop administration to the patient resulted in long-lasting remission of her ocular manifestations. Our findings suggest that AM eyedrops could represent an effective, non-invasive, simple-to-handle treatment for corneal lesions in patients with JEB and possibly other EB forms.
UbiComb: A Hybrid Deep Learning Model for Predicting Plant-Specific Protein Ubiquitylation Sites
Authors: Siraj Arslan , Lim Dae Yeong , Tayara Hilal , Chong Kil To
Published: May 2021

Protein ubiquitylation is an essential post-translational modification process that performs a critical role in a wide range of biological functions, even a degenerative role in certain diseases, and is consequently used as a promising target for the treatment of various diseases. Owing to the significant role of protein ubiquitylation, these sites can be identified by enzymatic approaches, mass spectrometry analysis, and combinations of multidimensional liquid chromatography and tandem mass spectrometry. However, these large-scale experimental screening techniques are time consuming, expensive, and laborious. To overcome the drawbacks of experimental methods, machine learning and deep learning-based predictors were considered for prediction in a timely and cost-effective manner. In the literature, several computational predictors have been published across species; however, predictors are species-specific because of the unclear patterns in different species. In this study, we proposed a novel approach for predicting plant ubiquitylation sites using a hybrid deep learning model by utilizing convolutional neural network and long short-term memory. The proposed method uses the actual protein sequence and physicochemical properties as inputs to the model and provides more robust predictions. The proposed predictor achieved the best result with accuracy values of 80% and 81% and F-scores of 79% and 82% on the 10-fold cross-validation and an independent dataset, respectively. Moreover, we also compared the testing of the independent dataset with popular ubiquitylation predictors; the results demonstrate that our model significantly outperforms the other methods in prediction classification results.
Acute Effects of Handheld Loading on Standing Broad Jump in Youth Athletes
Authors: Tai Wei-Hsun , Tang Ray-Hsien , Huang Chen-Fu , Lo Shin-Liang , Sung Yu-Chi , Peng Hsien-Te
Published: May 2021

The study aimed to investigate the acute effects of handheld loading on standing broad jump (SBJ) performance and biomechanics. Fifteen youth male athletes (mean age: 14.7 ± 0.9 years; body mass: 59.3 ± 8.0 kg; height: 1.73 ± 0.07 m) volunteered to participate in the study. Participants were assigned to perform SBJ with and without 4 kg dumbbells in a random order. Kinematic and kinetic data were collected using 10 infrared high-speed motion-capture cameras at a 250 Hz sampling rate and two force platforms at a 1000 Hz sampling rate. A paired t-test was applied to all variables to determine the significance between loading and unloading SBJs. Horizontal distance (p < 0.001), take-off distance (p = 0.001), landing distance (p < 0.001), horizontal velocity of center of mass (CoM; p < 0.001), push time (p < 0.001), vertical impulse (p = 0.003), and peak horizontal and vertical ground reaction force (GRF; p < 0.001, p = 0.017) were significantly greater in loading SBJ than in unloading SBJ. The take-off vertical velocity of CoM (p = 0.001), take-off angle (p < 0.001), peak knee and hip velocity (p < 0.001, p = 0.007), peak ankle and hip moment (p = 0.006, p = 0.011), and peak hip power (p = 0.014) were significantly greater in unloading SBJ than in loading SBJ. Conclusions: Acute enhancement in SBJ performance was observed with handheld loading. The present findings contribute to the understanding of biomechanical differences in SBJ performance with handheld loading and are highly applicable to strength and conditioning training for athletes.
Alcohol Use and Its Affordability in Adolescents in Slovakia between 2010 and 2018: Girls Are Less Adherent to Policy Measures
Authors: Ochaba Róbert , Baška Tibor , Bašková Martina
Published: May 2021

Background: The article analyzes selected indicators of alcohol use (weekly use, drunkenness within last month) and the ability of adolescents to buy alcohol in Slovakia between 2010 and 2018. Methods: Health Behavior in School-Aged Children (HBSC) is a cross-sectional questionnaire study. A standardized uniform questionnaire was used to create a representative sample of 15-year-old adolescents. Two surveys carried out in Slovakia in 2010 (n = 1568) and 2018 (n = 1298) were analyzed. Results: Weekly alcohol use and drunkenness declined only in boys, not in girls. Affordability of alcohol (not being prevented from buying it) declined among weekly drinking boys (from 60.4 to 34.1%) but remained almost unchanged in girls from a higher socioeconomic group compared to those from a lower one (57.9% vs. 30.6% in 2018). Conclusions: Affordability of alcohol in boys decreased with a decline in alcohol use, corresponding with implemented legislative measures. However, it remained unchanged in girls from a higher socioeconomic group.
Socioeconomic and Behavioral Correlates of COVID-19 Infections among Hospital Workers in the Greater Jakarta Area, Indonesia: A Cross-Sectional Study
Authors: Bella Adrianna , Akbar Mochamad Thoriq , Kusnadi Gita , Herlinda Olivia , Regita Putri Aprilia , Kusuma Dian
Published: May 2021

(1) Background: because of close contacts with COVID-19 patients, hospital workers are among the highest risk groups for infection. This study examined the socioeconomic and behavioral correlates of COVID-19 infection among hospital workers in Indonesia, the country hardest-hit by the disease in the Southeast Asia region. (2) Methods: we conducted a cross-sectional study, which collected data from 1397 hospital staff from eight hospitals in the Greater Jakarta area during April–July 2020. The data was collected using an online self-administered questionnaire and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) tests. We employed descriptive statistics and adjusted and unadjusted logistic regressions to analyze the data of hospital workers as well as the subgroups of healthcare and non-healthcare workers. (3) Results: from a total of 1397 hospital staff in the study, 22 (1.6%) were infected. In terms of correlates, being a healthcare worker (adjusted odds ratio (AOR) = 8.31, 95% CI 1.27–54.54) and having a household size of more than five (AOR = 4.09, 1.02–16.43) were significantly associated with a higher risk of infection. On the other hand, those with middle- and upper-expenditure levels were shown to have a lower risk of infection (AOR = 0.06, 0.01–0.66). Behavioral factors associated with COVID-19 infection among healthcare and non-healthcare workers included knowledge of standard personal protective equipment (PPE) (AOR = 0.08, 0.01–0.54) and application of the six-step handwashing technique (AOR = 0.32, 0.12–0.83). (4) Conclusion: among hospital staff, correlates of COVID-19 infection included being a healthcare worker, household size, expenditure level, knowledge and use of PPE, and application of appropriate hand washing techniques.
Does Regular Dancing Improve Static Balance?
Authors: Stawicki Przemysław , Wareńczak Agnieszka , Lisiński Przemysław
Published: May 2021

The aim of the study was to compare the static balance of dancers and non-dancers in a bipedal and unipedal stance. Twenty-three female dancers (mean age: 21.3 ± 1.7) and 24 age and sex-matched subjects (mean age 22.3 ± 1.0) participated in this study. A force platform was used to assess balance. The tests on the balance platform were performed in several positions with different foot placement, such as normal standing (NS) eyes open and eyes closed positions, semi-tandem position (ST), tandem position (TP), and one-leg standing (1L) eyes open and eyes closed position. Significant differences in balance between the dancers and the control group, especially in the tandem position and one-leg standing position with eyes closed were found. We observed higher results for the velocity of the COP in the frontal plane in the TP with a dominant limb in front (p = 0.04) and higher results for the velocity of the COP in the frontal plane (p = 0.01) and in the sagittal plane (p < 0.01) in the TP with a dominant limb in front in the control group. We also observed significant differences between groups in the mean velocity of COP sway in the sagittal plane in the 1 L position with eyes closed (p = 0.04). We concluded that dancing regularly for several years improves static balance.
Gynecology Meets Big Data in the Disruptive Innovation Medical Era: State-of-Art and Future Prospects
Authors: Khamisy-Farah Rola , Furstenau Leonardo B. , Kong Jude Dzevela , Wu Jianhong , Bragazzi Nicola Luigi
Published: May 2021

Tremendous scientific and technological achievements have been revolutionizing the current medical era, changing the way in which physicians practice their profession and deliver healthcare provisions. This is due to the convergence of various advancements related to digitalization and the use of information and communication technologies (ICTs)—ranging from the internet of things (IoT) and the internet of medical things (IoMT) to the fields of robotics, virtual and augmented reality, and massively parallel and cloud computing. Further progress has been made in the fields of addictive manufacturing and three-dimensional (3D) printing, sophisticated statistical tools such as big data visualization and analytics (BDVA) and artificial intelligence (AI), the use of mobile and smartphone applications (apps), remote monitoring and wearable sensors, and e-learning, among others. Within this new conceptual framework, big data represents a massive set of data characterized by different properties and features. These can be categorized both from a quantitative and qualitative standpoint, and include data generated from wet-lab and microarrays (molecular big data), databases and registries (clinical/computational big data), imaging techniques (such as radiomics, imaging big data) and web searches (the so-called infodemiology, digital big data). The present review aims to show how big and smart data can revolutionize gynecology by shedding light on female reproductive health, both in terms of physiology and pathophysiology. More specifically, they appear to have potential uses in the field of gynecology to increase its accuracy and precision, stratify patients, provide opportunities for personalized treatment options rather than delivering a package of “one-size-fits-it-all” healthcare management provisions, and enhance its effectiveness at each stage (health promotion, prevention, diagnosis, prognosis, and therapeutics).
Short-Time Recurrences of Plasmodium vivax Malaria as a Public Health Proxy for Chloroquine-Resistance Surveillance: A Spatio-Temporal Study in the Brazilian Amazon
Authors: Balieiro Antonio A. S. , Siqueira Andre M. , Melo Gisely C. , Monteiro Wuelton M. , Sampaio Vanderson S. , Mueller Ivo , Lacerda Marcus V. G. , Villela Daniel A. M.
Published: May 2021

In Brazil, malaria caused by Plasmodium vivax presents control challenges due to several reasons, among them the increasing possibility of failure of P. vivax treatment due to chloroquine-resistance (CQR). Despite limited reports of CQR, more extensive studies on the actual magnitude of resistance are still needed. Short-time recurrences of malaria cases were analyzed in different transmission scenarios over three years (2005, 2010, and 2015), selected according to malaria incidence. Multilevel models (binomial) were used to evaluate association of short-time recurrences with variables such as age. The zero-inflated Poisson scan model (scanZIP) was used to detect spatial clusters of recurrences up to 28 days. Recurrences compose less than 5% of overall infection, being more frequent in the age group under four years. Recurrences slightly increased incidence. No fixed clusters were detected throughout the period, although there are clustering sites, spatially varying over the years. This is the most extensive analysis of short-time recurrences worldwide which addresses the occurrence of P. vivax CQR. As an important step forward in malaria elimination, policymakers should focus their efforts on young children, with an eventual shift in the first line of malaria treatment to P. vivax.