bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative effectiveness of non-sedating antihistamines for patients with chronic spontaneous urticaria: a systematic review and network meta-analysis
Authors: QI FEI , TAN Yaqi , YAO Amin , FAN Jie , ZHAO Siqi , YANG Xutong , HE Yanling
Published: March 2022

Background Chronic spontaneous urticaria (CSU) is a kind of urticaria
that occurs independently without any exogenous stimulus. Non-sedating
H1-antihistamine is recognized as the first-line treatment option for
CSU. Objective To access the comparative efficacy of non-sedating
antihistamines in the treatment of CSU and to provide a relative ranking
of these treatments. Methods We enrolled randomized controlled trials
(RCTs) that compared a single non-sedating antihistamine with placebo or
anther single non-sedating antihistamine through searching databases:
CENTRAL, MEDLINE, and EMBASE; as well as the trial registries: ISRCTN,
ClinicalTrials. gov, ChiCTR, and ICTRP, etc. Two investigators evaluated
the eligibility, extracted the data, and assess the risk of bias of
included trials independently. A Bayesian network meta-analysis was
performed. Results We identified 42 RCTs with 8164 participants.
Twenty-six studies were included for the further network meta-analysis.
The odds ratio (95% credible intervals) of total overall symptoms
relief after 4-week treatment, the top three ordered from the most to
least effective non-sedating antihistamines were: loratadine 15.0 (2.9,
14.0), mizolastine 14.0 (2.9, 75.0), cetirizine 13.0 (4.4, 44.0).
Conclusions All enrolled non-sedating antihistamines were found to show
superiority over placebo in clinical outcomes in patients with CSU.
Loratadine, mizolastine, and cetirizine are the most efficacious drugs.
More head-to-head trials in a large number of patients are needed to
perform in the future.
Effect of Antimicrobial Stewardship Program on Carbapenems Consumption and Klebsiella Resistance in the Intensive Care Units
Authors: Elsawah Hozaifa , Samir Ahmed , Elrazzaz Mahmoud , Ramadan Abdallah , Elnaggar Amr , Taema Khaled
Published: March 2022

Introduction: The prevalence of carbapenem-resistant Klebsiella strains
and their related mortality are increasing worldwide. These pandemics
impose optimizing antibiotic consumption through the application of the
antibiotic stewardship programs. We aimed to evaluate the impact of
applying antibiotic stewardship programs on carbapenem consumption and
the klebsiella resistance pattern in critically ill patients of a large
Egyptian hospital. Method: We retrospectively evaluated critically ill
patients with isolated Klebsiella species from the ICU of Elaraby
hospital, Egypt during the period from April 2017 to January 2019. We
collected data related to carbapenem consumption (expressed as defined
daily dose /1000 patient-days) and Klebsiella clinical isolates and
their antimicrobial susceptibility pattern. Based on Klebsiella
sensitivity, as sensitive to ceftriaxone and cefotaxime, resistant to
ceftriaxone/cefotaxime, or resistance to meropenem/imipenem, Klebsiella
isolates were classified as grades 1, 2, or 3, respectively. Our primary
outcome was the change in carbapenem consumption after implementing the
program, while the secondary outcomes were the change in the incidence
of carbapenem-resistant Klebsiella. Results: The study included 205
patients with isolated Klebsiella species during the study period. The
antibiotic stewardship program started in March 2018. Out of the 205
patients, 61 patients (29.8%) represented the pre-intervention sample,
and 144 patients (70.2%) represented the post-intervention sample.
Applying the antibiotic stewardship program was associated with a
significant decrease in the carbapenem consumption from 38.9 to 26.6
defined daily dose /1000 patient-days (P=0.02). The incidence of
carbapenem-resistant Klebsiella was decreased from 85.25% of total
Klebsiella isolates to 48.6% (P<0.001). Klebsiella species
were more likely to be in a lower category of resistance after applying
the program with an odds ratio (OR) = 6.3 (2.88-13.73) using ordinal
logistic regression. Conclusion: Applying the antibiotic stewardship
program could reduce the unnecessary carbapenems use in the ICU with a
subsequent decrease in the emergence of the Klebsiella resistant
strains.
Fuzzy-I-Supra Compactness and Fuzzy-I-Supra Connectedness
Authors: Abbas fadhil
Published: March 2022

In this paper, we introduce the concept of fuzzy ideal supra compactness
and fuzzy ideal supra connectednessen in fuzzy supra topological spaces
and study some of the properties and characterizations of fuzzy ideal
supra compactness and fuzzy ideal supra connectednessen.
“Genital tuberculosis: is a key player for ectopic pregnancy in India: A cross-sectional observational study.”
Authors: Hooda Ruchi , Malik Sunita , Nair Deepthi
Published: March 2022

Background: Tuberculosis remains a major health problem in developing
countries and genital tuberculosis is responsible for affecting a
considerable number of women who presented in different manners as
chronic pelvic pain, pelvic inflammatory infection, infertility and
ectopic pregnancy. The average world-wide incidence of female genital
tuberculosis in infertile population has been reported as 5-10%, with
the range varying between <1% in USA and about 10% in India.
Objective: Present study is an attempt to find out the prevalence and
relationship of Genital tuberculosis(GTB) and ectopic pregnancy. Design:
It was a cross sectional case control study. Setting and population: The
study was conducted in department of Obstetrics and Gynecology in
association with Department of Microbiology, VMMC & Safdarjung
Hospital, and New Delhi including: • Case: Women (n=95) presenting with
ectopic pregnancy. • Control group: Women (n=95) subjected to tubal
ligation. Methods: After thorough history and examination, tubal sample
is collected from case and control group. Main outcome measures:
Prevalence of ectopic pregnancy with genital tuberculosis Results: The
prevalence of the genital tuberculosis in general population was 1.1%
and with ectopic pregnancy was 16.8%, that was quite significant so
genital tuberculosis should be kept in mind and punctiliously
anticipated in cases of ectopic pregnancy to prevent the countercoups of
genital tuberculosis. Conclusion: It is inferred that the prevalence of
ectopic pregnancy is higher among the women who had genital tuberculosis
and Funding : No funding received from any agency. Key Words: genital
tuberculosis, ectopic pregnancy, infertility, maternal morbidity
Effect of mixed-mode and surface-modified column chromatography on virus filtration performance
Authors: Shirataki Hironobu , Yokoyama Yoshiro , Oguri Ryota
Published: March 2022

Virus filtration is a critical process in the production of
biotherapeutics and drug products derived by plasma fractionation. The
processing steps upstream of virus removal filtration impact the
filterability (throughput and flux) of process solutions. We processed
mAb and plasma IgG spiked with aggregate by chromatography resins and
examined the filterability of the output on a virus filter (Planova
BioEX). The greatly reduced filterability for protein solutions with
aggregate was improved by processing with specific chromatography
resins. For mAb, mixed-mode AEX effectively reduced aggregate content
and significantly improved filterability. Mixed-mode AEX was also
effective for reducing aggregates in plasma IgG but modified CEX showed
even greater improvement in filterability. The results clearly show that
virus filter performance can be optimized by careful choice of column
chromatography. Finally, applying the throughput and flux from the virus
filter to four classical clogging models showed that mAb with aggregate
was best fit to the standard blocking model and plasma IgG with
aggregate was best fit to the complete blocking model, suggesting that
differences in solution properties result in different clogging
mechanisms.
Left bundle branch area pacing: Electrocardiographic features
Authors: DAS ASIT , Mandal Aniruddha , Chatterjee Suman
Published: March 2022

Background Left bundle branch (LBB) area pacing emerged as a promising
alternative to His bundle (HB) pacing in difficult cases of
physiological pacing and failed cases of cardiac resynchronization. So,
it is important to understand ECG features of LBB area pacing in various
subsets of patients. Objective We wanted to find out different
morphological patterns and characteristic ECG features of LBB area
pacing. Method Medtronic 3830 pacing lead was used to pierce the
interventricular septum 1-2 cm distal towards the RV cavity to a
previously placed electrophysiology catheter at distal HB region to
reach the LBB area in the right anterior oblique (RAO) 30 degree
projection. We observed paced QRS morphology in lead V1 and paced QRS
duration. Results We have analyzed ECG features of 60 patients who had
undergone LBB area pacing and 60 patients with RV apical pacing. LBB
area pacing resulted in narrower paced QRS complex than conventional RV
apical pacing. In patients with baseline LBBB QRS shortening from LBB
area pacing was more in comparison to patients with RBBB (34.45 ±8.07 ms
vs. 19.78±10.24 ms; p value 0.004). Paced QRS morphological pattern in
lead V1 was most commonly qR pattern followed by Qr pattern. Conclusions
LBB area pacing results in narrower paced QRS duration than RV apical
pacing. The morphological pattern is most commonly a qR or Qr pattern in
lead V1. Patients with baseline RBBB showed lesser paced QRS shortening
in comparison to patients with baseline LBBB.
Local injection of diluted vasopressin and aqua dissection at the time of modified Shirodkar cerclage: Description of a safe and efficient technique
Authors: Rishard Mohamed
Published: March 2022

Many modifications have been incorporated to original Shirodkar
technique to make the procedure an easy one whilst maintaining its
efficacy. Anecdotal evidence suggest that use of vasoconstrictors before
dissecting bladder base during Shirodkar method could be helpful. We
performed aqua dissection using diluted vasopressin during modified
Shirodkar cerclage in selected patients who required modified Shirodkar
method. Our experience shows that judicious use local injection of
diluted vasopressin to perform aqua dissection during modified Shirodkar
is a safe and efficacious method and would increase the overall quality
of the technique by reducing the blood loss and enhancing plane of
dissection.
LIFU-augmented Multifunctional Nanoparticles for Integrating Ultrasound Imaging and Synergistic Therapy of Metastatic Breast Cancer
Authors: Zhang Qian , Wang Wen , Shen Hongyuan , Tao Hongyu , Wu Yating , Ma Liyuan , Yang Guangfei , Chang Ruijiao , Wang Jiaxing , Zhang Hanfei , Wang Chenyu , Zhang Furong , Qi Jiaojiao , Mi Chengrong
Published: March 2022

The metastasis of breast cancer is believed to have a negative effect on
its prognosis. Benefiting from the remarkable deep-penetrating and
non-invasive characteristics, sonodynamic therapy (SDT) demonstrates a
whole series of potential leading to cancer treatment. To relieve the
limitation of monotherapy, a multifunctional nanoplatform has been
explored to realize the synergistic treatment efficiency. Herein, we
establish a novel multifunctional nano-system which encapsulates chlorin
e6 (Ce6, for SDT), perfluoropentane (PFP, for ultrasound imaging), and
docetaxel (DTX, for chemotherapy) in a well-designed PLGA core-shell
structure. The synergistic nanoparticles (CPDP NPs) featured with
excellent biocompatibility and stability which primarily enables its
further application. Upon low intensity focused ultrasound (LIFU)
irradiation, the enhanced ultrasound imaging could be revealed both in
vitro and in vivo. More importantly, combined with LIFU, the
nanoparticle exhibits intriguing antitumor capability through Ce6
induced cytotoxic reactive oxygen species (ROS) as well as DTX releasing
to generate a concerted therapeutic efficiency. Furthermore, this
treating strategy actives a strong anti-metastasis capability by which
lung metastatic nodules have been significantly reduced. The results
indicate that the SDT-oriented nanoplatform combined with chemotherapy
could be provided as a promising approach in elevating effective
synergistic therapy and suppressing lung metastasis of breast cancer.
A REVIEW ON THE BIOACTIVITY AND POTENTIAL HEALTH BENEFITS OF VITEX AGNUS‐CASTUS LINN
Authors: Basharat Shahnai , Abid Fatima , Iftikhar Faiza , Wahid Aiman , Gilani Muhammad , Ashraf Anum , Masood Sara , Jaffar Madiha , Noreen Sana , Nisar Tehreem
Published: March 2022

Vitex agnus-castus Linn referred as a small decides tree commonly known
as the chaste tree. The fruits of this tree have been used as a
therapeutic agent for over 2500 years in ancient Egypt, Greece, Iran,
and Rome. The Vitex Agnus belongs to the family of Verbenaceae which
encompasses 250 species from all over the world and Vitex is the largest
genus of this family. Vitex has many therapeutic and medicinal benefits
the German health authorities have approved the utilization of V.agnus
castus for treating irregularities of menstruation among females and
also associated disorders, mastalgia, and menopausal disorders. Evidence
based studies have proved multiple therapeutic and pharmaceutical
benefits of this medicinal plant which includes antioxydant,
anti-cancer, immunomodulatory antimicrobial, anti-inflammatory,
anti-fungal, insect repellent, fracture healing, osteopenic, epileptic,
anti-diabetic and it also prevents from nonalcoholic fatty liver
disorders. The literature also suggests that there is no drug
interaction associated with Vitex. Moreover, the use of V. agnus-castus
is contraindicated (or ought to be abstained from) during pregnancy
because of hypothetical sentiments and cell examines that displayed
progesteronic and estrogenic properties of the plant. This review gives
the documentation to researchers to utilise the properties of V.
agnus-castus as a powerful natural medication and its wellbeing and
satisfactory measurement are examined. Progressed preclinical and
clinical examinations for adequately evaluating the security and helpful
viability of V. agnus-castus are suggested.
Levosulpiride associated neuroleptic malignant syndrome in an elderly patient: A tale of confusing brand names
Authors: Kaur Upinder , Acharya Kumudini , Singh Amit , Gambhir Indrajeet , Chakrabarti Sankha Shubhra
Published: March 2022

Neuroleptic malignant syndrome(NMS) is a lethal idiosyncratic reaction
characterized by fever, rigidity, altered sensorium and autonomic
disturbances. Antidopaminergic drugs such as haloperidol, dopamine
depletors like reserpine and atypical antipsychotics such as
risperidone, are the major drugs implicated in NMS. Levosulpiride
belongs to the benzamide class of antiemetics and is a blocker of
dopamine(D2) receptors. The drug is available in India, Korea and some
European countries. Here we present an interesting case of Levosulpiride
associated neuroleptic malignant syndrome in an elderly female. The case
also reflects how a minor difference in the brand names can produce a
major debilitating cascade in geriatric patients.
Early Report of BCG Group Against COVID-19: Initial Results of the Volunteer BCG Vaccination
Authors: Ozkaya Sevket , Karaman Irem , Uzar Tugce , Dirican Adem
Published: March 2022

Objective: This article aimed to report the potential protective effect
of BCG vaccine against COVID-19 by presenting the follow-up report of
individuals who voluntarily receive BCG vaccination (BCG group). In this
direction, preliminary data from BCG group regarding participants’
SARS-CoV-2 infection status, and the side effects with complications of
BCG vaccination is reported. Method: A total of 84 health professionals
and their relatives who voluntarily received BCG vaccine included in the
study. Participants completed an online questionnaire that consists of
14 questions regarding their characteristics, SARS-CoV-2 infection
status, and effects of BCG. Results: 63 participants were healthcare
workers, and 21 participants were relatives of a healthcare worker.
81(96.4%) participants stated that they consider themselves in the
high-risk group for getting infected with SARS-CoV-2. 69(82.1%)
participants declared that they had been in the same environment and
contacted with a COVID-19 patient. 82(97.6%) participants had at least
one BCG scar from childhood. 21(25%) participants declared one
complication after BCG vaccination. As a complication, 8(38.1%)
participants had local non-permanent sterile abscess, and 13(61.9%) of
them had weakness, joint pain, and subfebrile fever. 31(36.9%)
participants had a PCR test for COVID-19 at least 4 weeks after BCG
revaccination, and the results of all 31 participants were negative.
82(97.6%) participants declared that they felt more protected against
COVID-19 after BCG vaccination. Conclusion: Results from this study
suggest that it is beneficial to administer BCG vaccine to protect the
high-risk group against COVID-19 with no side effects and risks.
The Identification of Novel Inhibitors of Human Angiotensin-converting Enzyme 2 and Main Protease of Sars-Cov-2: A Combination of in silico Methods for Treatment of COVID-19
Authors: Zarezade Vahid , Rezaei Hamzeh , Shakerinezhad Ghodratollah , Safavi Arman , Nazeri Zahra , Veisi Ali , Azadbakht Omid , Hatami Mahdi , Sabaghan Mohamad , Shajirat Zeinab , Zarghooni Maryam
Published: March 2022

The angiotensin-converting enzyme 2 (ACE2) and main protease (MPro), are
the putative drug candidates for the severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2). In this study, we performed 3D-QSAR
pharmacophore modeling and screened 1264479 ligands gathered from
Pubchem and Zinc databases. Following the calculation of the ADMET
properties, molecular docking was carried out. Moreover, de novo ligand
design was performed. MD simulation was then applied to survey the
behavior of the ligand-protein complexes. Furthermore, MMPBSA was
utilized to re-estimate the binding affinities. Then, a free energy
landscape was used to find the most stable conformation of the
complexes. Finally, the hybrid QM-MM method was carried out for the
precise calculation of the energies. The Hypo1 pharmacophore model was
selected as the best model. Our docking results indicate that the
compounds ZINC12562757 and 112260215 were the best potential inhibitors
of the ACE2 and MPro, respectively. Furthermore, the Evo_1 compound
enjoys the highest docking energy among the designed de novo ligands.
Results of RMSD, RMSF, H-bond and DSSP analyses have demonstrated that
the lead compounds preserve the stability of the complexes’ conformation
during the MD simulation. MMPBSA data confirmed the molecular docking
results. The results of QM-MM showed that Evo_1 has a stronger
potential for specific inhibition of MPro, as compared to the 112260215
compound. In conclusion, our results showed that the de novo designed
Evo_1 compound has the potential to be used as a drug for the treatment
of COVID-19; however, further in vitro and in vivo validations are
required.
The efficacy and safety of high-power ablation compared to conventional power for pulmonary vein isolation in patients with atrial fibrillation: a systematic review and meta-analysis
Authors: Song Yuanbin , Li Jiabei , Zhang Yi , Zhang Bo , Tan Hu
Published: March 2022

Aims This study aimed to compare the efficacy and safety of high-power
(HP) ablation to conventional power (CP) in patients undergoing
pulmonary vein isolation (PVI) for atrial fibrillation (AF). Methods and
Results A systematic literature search was performed using the Medline
and EMBASE databases up to February 2020 without language restrictions.
Studies comparing HP (≥ 50W) with CP (≤ 40W) radiofrequency ablation
(RFA) for circumferential PVI were included. The primary efficacy
endpoint was the rate of freedom from AF recurrence. The first-class
isolation rate, the percentage of acute pulmonary vein reconnections
(PVR), RFA time, and procedure time were assessed as secondary efficacy
outcomes. Safety was measured as the incidence of complications. In
total, 10 eligible studies (2912 patients) were included. The rate of
freedom from AF recurrence was similar between the two groups (odds
ratio (OR): 1.34, 95% confidence interval (CI): 0.94－1.91; P = 0.10).
The first-class isolation rate was significantly higher in the HP group
(OR: 2.65, 95% CI: 1.72－4.09; P < 0.001). The HP group
showed significantly less acute PVR (OR: 0.19, 95% CI: 0.07－0.49; P
< 0.001). RFA time and procedural time were significantly
shorter in the HP group. The percentage of pericardial tamponade was
similar in both groups. Conclusions RFA with HP did not improve the rate
of freedom from AF recurrence, but significantly shortened RFA time and
procedure time with a significantly higher rate of first-class PVI and
lower rate of acute PVR. The HP ablation strategy demonstrated a
comparable safety profile to CP ablation.
Pregnancy drug use in Beijing district during 2016-2018: an analysis of data from Beijing Prescription Evaluation System
Authors: Du Boran , Shi Xiangjun , Song Meijing , Yue Xiaolin , Bian Baosheng , Cao Qingheng , Yin Chenghong , Feng Xin
Published: March 2022

Aims: To investigate the characteristics of prescriptions during
pregnancy from Beijing Prescription Comment System from 2016 to 2018.
Methods: Data was extracted and selected in the system with key words
according to inclusion criteria. We examined patterns and assessed risks
with FDA category categorization. Factors associated to D/X, C, N/A
categories were explored through multivariate logistic regression. We
re-classified the drugs with Briggs categorization of B, C, D, N/A
categories. Results: From 5,446,561 prescriptions of Beijing
Prescription Comment System, 647 varieties of chemical or biomedical
drugs with 112,566 prescriptions were finally included. The
prescriptions majorly distributed on patients from 25 to 35 years
(68.17%), in 1st and 2nd trimesters (75.05%). Drugs in FDA category
were followed by B (23.83%), C (13.37%), X (6.17%), A (3.26%), D
(1.7%), while 51.68% not available. In D/X categories, estrogens,
assisted reproductive drugs and imaging testing drugs were most. Within
X category, prescriptions showed an increase in women under 25 years
from 216 to 2018. In drugs of D/X categories, gestational week under 12
weeks (OR=3.259, 95%CI, 1.441-7.37) matters most among risk factors of
specialized hospitals, gestational age between 30 and 35, and
non-obstetrics and gynecology departments. Under Briggs categorization,
FDA category C contains more risk drugs in animal or human tests
(74.25%) according to Briggs categorization. Conclusions: This research
explored exposure of pregnant women to potential risk drugs by FDA
category and Briggs categorization. Main characteristics and risk
factors revealed by this study should be monitored in the ongoing
studies on pregnancy drug safety.
Parametric Analysis of the Heat transfer behavior of the Nano-particle ionic-Liquid Flow between Concentric Cylinders
Authors: Shah Rehan Ali , Ullah Hidayat , Khan Muhammad , Khan Aamir
Published: March 2022

The paper urges to investigate the enhanced viscous behavior and the
heat transfer phenomenon of the unsteady ionic-nano-liquid squeezing
flow between the two infinite parallel concentric cylinders. The
physical scenario is transformed into a partial differential equation
system by the modified Navier stokes equation, the Poisson equation, the
Nernst-Plank equations, and the energy equation. The PDE’s system is
transformed into the coupled ODE’s system by opting for
suitable/applicable transformation. The solution of the coupled
differential equations system is carried out by the Parametric
continuation method (PCM)and BVP4c Matlab based methods. The effect of
the squeeze number, the nanoparticle volume fraction, the Prandtl
number, Schmidt number, and the heat source parameter on nano-ionic
liquid flow, heat transfer rate, and other physical variables are
tabulated, graphed, and discussed. The analysis of the Nusselt number
for Cu-water, Al2O3-water, and TiO2- water in response to the Prandtl
number(Pr), the Squeeze number(S), and the heat source parameter(Hs)
concludes that the Cu-nanoparticle is the best choice for the heat
transfer function through the contracting flow channel with the
increasing heat transfer parameter(Hs). It is also noticed that the
thermal boundary layer tends to decrease for the increasing Prandtl
number(Pr) in Cu-water, Al2O3-water, and TiO2-water, which indicates
that the convection mode of heat transfer is not efficient in this whole
flow set-up. Applications of this study involved in the demands to
understand the interfacial electrokinetic phenomena in the liquid
transport processes which increase significantly as various
Micro-Electro-Mechanical Systems (MEMS) and MicroFluidic devices involve
liquid transport processes.
Prediction of Gestational Age: A Comparison of Regression Models
Authors: Das Kalyan , Das Anisha , Sultana Aysha , Khan M. Salauddin , Biswas M. Haider Ali
Published: March 2022

Aim: The main objective is to incorporate the major foetal parameters –
biparietal diameter, head circumference, abdominal circumference and
femur length for prediction of gestational age through ultrasonography
between 10th and 42nd weeks of gestation and try to do a simultaneous
comparative study with gestational age predicted by last menstrual
period. Methods: The study has been conducted particularly on the
population of Bangladesh. It has been done on 229 Bangladeshi women who
had usual singleton foetuses, with evidence of menstrual dates by
sonography before fourteen weeks of gestation. Foetal anatomical
structures have been scanned and measured at the time of sonographic
inspection. For each patient, in addition to the four foetal parameters
such as Biparietal Diameter (BPD), Head Circumference (HC), Abdominal
Circumference (AC) and Femur Length (FL), the other parameters like
Gestational Age (GA) by Last Menstrual Period (LMP) as well as by
Ultrasonography (USG) have been recorded. Here we have adopted
non-linear regression models in order to predict the response on
gestational age. Usually, different modelling methods have been used for
this purpose. Results: The logarithmic models normally presented better
results if gestational age was predicted based on a single parameter
than polynomial models whereas if all predictor variables were
considered together, then Nernst model may turn out to be the best one.
Also, it was seen that the accuracy level of gestational age predicted
by ultrasonography was slightly more accurate than that determined by
last menstrual period. Conclusions: There is a high degree of
association among the different foetal parameters. Further, there is a
high degree of association between the gestational ages by LMP and that
by USG. Prediction of gestational ages by USG technique gives a good
degree of accuracy and hence can be a reliable technique for estimation
of gestational ages.
STUDY TO ESTABLISH GENETIC ASSOCIATION OF CARDIAC CONDUCTION DEFECT IN INDIAN PATIENTS UNDERGOING PACEMAKER IMPLANTATION
Authors: GARDE SWAPNIL , BHATTACHARYYA PRANAB , IDRIS MOHAMMAD , HAZARIKA GAUTAM , DABU ROMAR
Published: March 2022

AIMS: To study the genetic association of cardiac conduction defects
(CCD) by evaluating Single nucleotide polymorphism(SNP) in genes of
SCN1B and KCNJ2 and to evaluate baseline characteristics between cases
and controls. METHODS AND RESULTS: Case group consisted of 81
individuals with diagnosis of conduction disturbances who underwent
permanent pacemaker implantation. . The control group consisted of 79
unrelated individuals above 18 years of age of the local population not
having a present or past personal or family history (first degree
relatives) of any cardiac ailment especially cardiac conduction defects.
Isolation of genomic Deoxyribonucleic acid(DNA) from all samples was
done, Genomic DNA was checked to ensure the presence of intact DNA .
SCN1B : SNP rs55742440 have no bearing on the protein except in
producing a splice variant . SNP rs67701503 does not lie in the splice
site region, not having any significance in the regulation of the gene
as well. NetGene2 analysis of SNP rs67486287 negates its presence in the
splice site. KCNJ2 :SNP rs199473653 is leading to a missense amino acid
change resulting in homozygous GG variant found in almost equal
frequency in both groups. SNP rs199473653 gene has not been reported as
a disease-causing mutation. CONCLUSION:The alteration of nucleotide in
SCN1B intron (SNP rs55742440, rs67701503, rs 67486287) between cases and
controls was found to have no odds of affecting the outcome of CCD.
There was no variation or alteration in nucleotide bases of KCNJ2 (SNP
rs786205813, rs199473653) between the groups.
Existence of solution of infinite systems of nonlinear fractional integral equation of two variables in $C(I\times I,m(\Delta_{v}^{u}, \phi,p))$
Authors: Kayvanloo Hojjatollah Amiri , allahyari Reza , allahyari Asghar
Published: March 2022

The aim of the present paper, is to investigate the solvability of
infinite systems of nonlinear fractional integral equation of two
variables in $C(I\times
I,m(\Delta_{v}^{u}, \phi,p))$ by
using the Hausdorff measure of noncompactness with the help of
Meir–Keeler condensing operators. We also provide an illustrative
example in support of our existence theorems.
Soil water dynamics under vegetation based on a contrastive experiment between vegetated and non-vegetated sites
Authors: Zhao Ming , Wang wenke , Ma Zhitong , Wang Qiangmin , Wang Zhoufeng , Chen li , Fu Bowen
Published: March 2022

Vegetation plays an active role in soil water dynamics and water balance
in atmosphere-soil-vegetation-groundwater system. Therefore, we
characterised soil water transportation at depths of 0-4.0 m, induced by
root water uptake of groundwater-dependent Salix across a whole growth
stage, as well as taking place in non-vegetated soil, in the semi-arid
Ordos basin of China. The results show that: soil moisture content
showed clear heterogeneity under the combined influence of evaporation,
rainfall and root water uptake. Thus, the soil profile was divided into
the climate impacted layer, the transitional layer, and groundwater
supporting layer. Root water uptake increased the variability of soil
water in the vadose zone and changed the classification structure. The
impacts of different rainfall regimes on soil moisture and vegetation
response at the individual plant scale were systematically analysed. The
rainwater infiltration hysteresis as connected to rainfall intensity,
soil depth and the roots-system preferential channel were confirmed, as
was the canopy-shading effect. Further, an exponential-logarithmic
normal composite root water uptake distribution was obtained, via an
inverse method. Of the accumulated 356.3 precipitation in 2016, the
annual soil water increase was 97.9 mm for bare site, increased by
10.2% in total, accounting for 25.5% of the precipitation of that
year. Conversely, for vegetated site, annual soil water decrease
registered at 254.40 mm, decreased by 28.3% in total. The contrastive
experiment indicates that root water uptake processes change the soil
water flow field and aggravate water scarcity, resulting in soil
desiccation in the deep local soil layers and groundwater depletion. The
dried soil layers blocked water interchange, which is extremely
detrimental to ecohydrological processes. Our results provide a
scientific basis within which the hydrodynamic processes of vegetation
in semi-arid regions may better be understood and inspire research to
find a balance between groundwater management and vegetation replanting.
Flu-IV score: a predictive tool for assessing the risk of invasive mechanical ventilation in patients with influenza-related pneumonia
Authors: Liang Chen , Han xiudi , Li Yanli , Zhang Chunxiao , Xing Xiqian
Published: March 2022

Aim: To develop an assessment tool to predict invasive mechanical
ventilation (IMV) among influenza-related pneumonia (Flu-p) patients
within 14 days of admission. Methods: In total, 1107 Flu-p patients from
five teaching hospitals were retrospectively enrolled from January 2012
- December 2019 and used to develop a predictive model. Results:
Overall, 10.6% (117/1107) of patients underwent IMV within 14 days of
admission. Multivariate regression analyses revealed that the following
factors were associated with IMV: early neuraminidase inhibitor use (-2
points), lymphocytes < 0.8×109/L (1 points), multi-lobar
infiltrates (1 point), age ≥ 65 years old (2 points), systemic
corticosteroid use (2 points), PaO2/FiO2 < 300 mmHg (2
points), respiratory rate ≥ 30 breaths/min (3 points), and arterial PH
< 7.35 (3 points). A total score of six points was used to
identify patients at risk of IMV. This model had a sensitivity of
88.79%, a specificity of 87.55%, and exhibited better predictive
performance than the ROX index (AUROC = 0.927 vs 0.688, p <
0.001), modified ROX index (AUROC = 0.927 vs 0.747, p <
0.001), and HACOR scale (AUROC = 0.927 vs 0.524, p < 0.001).
Conclusions: Flu-IV scores can be used to reliably predict 14-day IMV
rates in Flu-p patients.
Successful treatment of acute myocardial infarction induced by Left bundle branch pacing: a case report
Authors: Gao Bingbing , Li Li , Han Xuebin , An Jian , Li Tianliang , Li Xin , Guo Caixia , Zhang Jie
Published: March 2022

Acute myocardial infarction is mostly caused by coronary atherosclerotic
stenosis or thrombosis. Myocardial infarction directly caused by left
fascicular pacing is rarely reported.We reported a 60-year-old male
patient who accepted left bundle branch pacing (LBBP). After operation,
this patient experienced acute myocardial infarction (AMI), Adams-stokes
syndrome and was treated successfully at last.
A Case of Quadricuspid Aortic Valve Regurgitation: The Cardiac Clover
Authors: Dayco John , Cardozo Shaun
Published: March 2022

A quadricuspid aortic valve is a very rare congenital heart condition
that can present as aortic regurgitation in the 5th and 6th decade of
life. The following case report will describe a patient who presented
with symptoms of severe aortic regurgitation and was found to have a
quadricuspid aortic valve on echocardiography. The case will describe
the clinical manifestations in which the patient presented and the
subsequent diagnosis of the quadricuspid aortic valve. The rationale for
surgical approach will also be discussed, along with the patient’s
clinical response.
How to cope with drought and not die trying: soluble sugars and drought acclimation across tree species with contrasting niche breadth
Authors: Fajardo Alex , Piper Frida
Published: March 2022

Worldwide drought events causing tree growth decline and mortality are
altering the carbon (C) balance of forest ecosystems. One unexplored
aspect of trees’ vulnerability is whether responses to drought may be
linked to species’ niche breadth. Using the most severe 2015-2016 El
Niño drought event in the last 70 years in Patagonia, we determined pre-
and post-drought growth, C reserves, wood isotopes, and vessel diameter
in eight angiosperm tree species of contrasting niche breadth across a
sharp precipitation gradient in southern Chile. All species responded in
unison after the drought with a non-water-conservative response,
maintaining growth and C reserves, decreasing δ13C, and increasing both
vessel diameter and the soluble sugars:starch ratio relative to
pre-drought. We unequivocally showed a functional coordination of
organisms’ vital traits, a drought-induced acclimation based on starch
conversion into soluble sugars in all of the tree species we examined,
regardless of their niche breadth and habitat preference.
An Assessment of Relation of Environmental Parameters and COVID-19 transmission at the early stage during March-May 2020 in India
Authors: GOUDA KRUSHNA , Benke Mahendra , SINGH PRIYA , KUMARI Reshama , P Nikhilasuma , Agnihotri Geeta , JOSHI SNEH , S HIMESH
Published: March 2022

The Corona virus disease 2019 (COVID-19) mainly caused by the novel
severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) became a
global pandemic by March 2020. Actual there is no strong evidence of
weather and COVD-19 spread relation as it is a new virus. This study is
mainly focussed on the tropical weather impact on the spatio-temporal
spread of COVID-19 during the early stages i.e. March-May 2020 in India,
which is a large country where the disease has shown an exponential
growth. This study is an attempt to assess the relationship of major
environmental parameters like solar radiation, air temperature and
humidity with the positive cases of COVID-19 for the period March-May
2020 which is the summer season or pre-monsoon season over India. The
time series and significant correlation analysis at daily, weekly scale
and the spatial analysis of weather and COVID-19 cases are presented.
The results show significant correlation of solar radiation and
atmospheric temperature with COVID-19 cases both at daily and weekly
scale in India whereas humidity has low correlation in the study period.
But the temperature humidity index (THI) a measure of the thermal stress
shows positive correlation with the disease spread. These results can be
a good input for developing the integrated modelling framework for the
COVID-19 forecasting using state of art numerical weather prediction
model and disease process modelling.
Trauma and pregnancy: is flow cytometry detection and quantification of fetal red blood cells useful? A retrospective cohort study
Authors: Rottenstreich Misgav , Meir Reut , Glick Itamar , Alexandroni Heli , Schwarz Alon , Broide Ellen , Grisaru-Granovsky Sorina , Sela Hen
Published: March 2022

Objective: To assess whether positive flow cytometry quantification of
fetal red blood cells is associated with adverse outcomes in cases of
mild trauma during pregnancy. Design: A retrospective computerized
database cohort. Population: Pregnant women with viable gestation
involved in trauma who underwent flow cytometry. Flow cytometry was
considered positive (≥0.03/≥30 ml). Methods: A univariate analysis was
followed by a multivariate analysis. Main outcome measures: Composite
adverse maternal and neonatal outcome was defined as one or more of the
following: intrauterine fetal death, placental abruption, pre-term birth
< 37 weeks of gestation, immediate premature rupture of the
membranes, and immediate delivery following trauma. Results: During the
study 1023 women met inclusion and exclusion criteria. Among the cohort,
119 women (11.6%) had positive flow cytometry (≥0.03/≥30 ml) with
median result of 0.03 [0.03-0.04], while 904 women (88.4%) had
negative flow cytometry test result (≤0.03/≤30 ml) with median result of
0.01 [0.01-0.02]. Composite adverse outcome occurred in 8% of the
women, with no difference in the groups with vs. without positive flow
cytometry (4.2% vs. 8.5%; p=0.1). Positive flow cytometry was not
associated with any adverse maternal or neonatal outcome. This was
confirmed on a multivariate analysis. Conclusions: Flow cytometry result
is not related to adverse maternal and fetal/neonatal outcome of women
involved in minor trauma during pregnancy. We suggest that flow
cytometry should not be routinely assessed in pregnant women involved in
minor trauma.
Development of Daphnia magna SSR markers and genetic diversity analysis based on RAD-seq technology
Authors: Shengman Zhang , Zheng Haisu , Wu Meiqin , Tan Meng , Chen Siwei , Han Zheng , Liu Jinlin , He Peimin , He Wenhui
Published: March 2022

Daphnia magna belongs to the Cladocera order and plays an important role
in the water ecosystem. With the intensification of water pollution, the
wild population of D. magna has declined rapidly in recent years, and
insufficient molecular markers have limited effective research and
conservation of this species. In our research, 26 novel microsatellite
(SSR) markers were developed in an artificially domesticated of D. magna
and 12 wild population of D. magna using restriction site-associated DNA
sequencing (RAD-seq). The results showed that the observed
heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.083
to 0.999 and 0.085 to 0.862, respectively. The PIC ranged from 0.368 to
0.805. These results indicate that the developed SSR marker is highly
polymorphic. Nei’s genetic identity (H) ranged from 0.0926 to 0.3462,
with a mean of 0.2233. Shannon’s Information index (I) ranged from
0.1333 to 0.4799, with an average of 0.3073; Shanxi province had the
highest value and Hunan province had the lowest. Genetic distance and
Nei’s genetic identity analysis, NJ tree diagram analysis, and PCoA
analysis were conducted on populations of Daphnia magna from different
regions. An analysis of population genetic structure divided the test D.
magna samples into two major groups. There was no absolute correlation
between the gene distribution of D. magna and its geographic origin.
These results indicate that the genetic diversity of D. magna is rich,
and the genetic structure of D. magna differs considerably in different
regions. These research results and the newly developed polymorphic SSR
markers for D. magna are of great significance in terms of the genetic
breeding of D. magna, identification of wild and artificially
domesticated species and conservation genetics research.
Toxic study on the new insulating gas trifluoromethanesulfonyl fluoride: a substitute to SF6
Authors: Zhang Lei , Fan Guozhi , Peng Ruichao , Gao Zhanyang , Luo Yunbai , Song Guangsen , Wang Yi
Published: March 2022

It has been proven that the gas trifluoromethanesulfonyl fluoride
(CF3SO2F) has good dielectric performance and potential to replace
strong greenhouse gas sulfur hexafluoride (SF6), which is the most
commonly used insulating gas in electrical engineering area. In this
paper, acute toxic gas inhalation test was carried out. The results
showed that the lethal concentration 50% (LC50) for female rats was
27.1 g/m3 and 23.3 g/m3 for male rats. The behavioral and vital sign
changes in the rats were recorded. Pathological sections of the main
organs revealed that the heart, lungs, spleen and eyes suffered the most
damage from the gas. This research also provides scientific suggestions
for the protection in practical engineering.
Gastrin/CCK2R alleviates mucus barrier loss via β-arrestin1/NF-κBp65 signaling in ulcerative colitis.
Authors: Tan Siwei , Peng Xiuying , Liu Xianzhi , Tan Shuyan , Huang Xiaoli , Pan Xuemei , Jiang Jie , Liu Huiling , Tao Jin , Wu Bin
Published: March 2022

Background and Purpose: The defective colonic mucus barrier is a feature
of ulcerative colitis (UC) that enables increased bacterial contact with
the epithelium, which triggers mucosal damage, and gastrin has been
reported to be able to promote healing through the cholecystokinin 2
receptor (CCK2R) signaling to increase epithelial regeneration and
protect against colonic injury. However, the role of gastrin in UC
remains unclear. Experimental Approach: Colonic samples from human
sections and mouse models using β-arrestin1 wild-type
(β-arr1-WT) and β-arrestin1 knockout (β-arr1-KO)
littermates, intestinal epithelial cells specific NF-κBp65
deletion (NF-κBp65) and wild-type
(NF-κBp65) mice were analyzed. The
mucosal injury, goblet cells status, MUC2 expression and bacteria
penetration/colonisation were examined, and the effect of gastrin in
colitis was also investigated. Key Results: We demonstrate that mucus
barrier loss and bacterial colonisation of the crypts were observed in
colitis, and exogenous gastrin could restore the mucus barrier, reduce
bacterial colonisation of the colonic crypts and alleviate colitis via
CCK2R. Furthermore, targeting CCK2R by YF476, β-arrestin1
(β-arr1) deletion or intestinal epithelial NF-κBp65
deficiency breached gastrin-mediated mucus barrier restoration and
mucosal protection in colitis. Conclusion and Implications: These data
demonstrate that gastrin alleviates mucus barrier loss and bacterial
colonisation of the colonic crypts via CCK2R/β-arr1/NF-κBp65 signaling
in colitis, and this network may be a potential therapeutic target for
UC.
Effect of nitrous oxide use on external cephalic version success rate; A systematic review and meta-analysis
Authors: Massalha Manal , Izhaki Ido , Salim Raed
Published: March 2022

Background Several adjuvant interventions have been evaluated for
improving the success rate of external cephalic version (ECV) and reduce
the rate of cesarean delivery (CD). Evidence regarding the effect of
Nitrous oxide (NO) is limited with inconsistent results on pain score
and success rate. Objective To examine the effect of inhaled NO on the
success rate and pain score for women undergoing ECV. Search strategy
MEDLINE, EMBASE, PubMed, Ovid Medline, ClinicalTrials.gov, the Cochrane
Library and Google Scholars from inception till June 2020. Selection
criteria Peer-review studies that examined the success rate of inhaled
NO use during ECV attempt compared with or without the use of other
analgesic agents were eligible for inclusion. Data collection and
analysis All ascertained records were screened independently for
eligibility by two authors. The study quality scores were evaluated as a
source of heterogeneity by ﬁtting meta-regression models to the
individual study effect sizes. Results Of the 186 records, two
randomized trials and one prospective cohort study (434 in the NO group
and 286 in the controls) were deemed adequate for meta-analysis. ECV
success rate did not differ significantly between the NO and the
controls group (p=0.825; OR 1.036; 95% CI, 0.756-1.419). In addition,
the use of NO did not affect pain scores (p=0.457; OR 0.759; 95% CI,
-1.240-2.759) and there was no difference in the incidence of CD
(p=0.943; OR 1.013; 95% CI, 0.703-1.46). Conclusion Use of NO during
ECV is not associated with increase in ECV success rate and does not
affect pain score.
Is it a myocardial hematoma or a pseudoaneurysm of left ventricle? Analysis of a rare complication of radiofrequency catheter ablation of the heart
Authors: Zhang minyu , Gao Yu , Wei Wei , Pan Tao , Wang jianhua
Published: March 2022

This is a case of left ventricular pseudoaneurysm with intratumoral
thrombus resulting from cardiac radiofrequency surgery. Due to the small
size of the tumor, the patient recovered after half a year. This paper
introduces in detail the whole process of echocardiography follow-up of
pseudo ventricular aneurysm, and emphasizes that bedside
echocardiography should be carried out actively when patients have
uncomfortable symptoms during the operation.
Alternative Position of Cannulae in Veno-venous Extracorporeal Membrane Oxygenation
Authors: Go Ui Won , Choi Chang Hyu , Park Chul Hyun , Lee Seok In
Published: March 2022

Optimal positioning of drainage and return cannulae is critically
important for minimizing the recirculation in patients with respiratory
failure requiring veno-venous extracorporeal membrane oxygenation (VV
ECMO). If a drainage cannula is positioned at a low level of right
atrium through the femoral vein, flow disturbance due to drainage
chattering can occur when the intravascular volume is insufficient, or
intrathoracic or intraabdominal pressure is increased. We report a case
in which drainage chattering was eliminated and recirculation were
decreased by repositioning the drainage and return cannula in a patient
with VV ECMO flow disturbance when intrathoracic pressure was increased.