bluuebunny/crossref_metadata_2025 huggingface dataset:
Unexpected Anisotropy of the Electron and Hole Landé g-Factors in Perovskite CH3NH3PbI3 Polycrystalline Films
Authors: Garcia-Arellano Guadalupe , Trippé-Allard Gaëlle , Campos Thomas , Bernardot Frédérick , Legrand Laurent , Garrot Damien , Deleporte Emmanuelle , Testelin Christophe , Chamarro Maria
Published: April 2022

In this work, we studied, at low temperature, the coherent evolution of the localized electron and hole spins in a polycrystalline film of CH3NH3PbI3 (MAPI) by using a picosecond-photo-induced Faraday rotation technique in an oblique magnetic field. We observed an unexpected anisotropy for the electron and hole spin. We determined the electron and hole Landé factors when the magnetic field was applied in the plane of the film and perpendicular to the exciting light, denoted as transverse ⊥  factors, and when the magnetic field was applied perpendicular to the film and parallel to the exciting light, denoted as parallel  ∥  factors. We obtained |ge,⊥|=2.600 ± 0.004, |ge,∥|=1.604 ± 0.033 for the electron and |gh,⊥|=0.406 ± 0.002, |gh,∥|=0.299 ± 0.007 for the hole. Possible origins of this anisotropy are discussed herein.
A Plasmonic Biosensor Based on Light-Diffusing Fibers Functionalized with Molecularly Imprinted Nanoparticles for Ultralow Sensing of Proteins
Authors: Arcadio Francesco , Seggio Mimimorena , Del Prete Domenico , Buonanno Gionatan , Mendes João , Coelho Luís C. C. , Jorge Pedro A. S. , Zeni Luigi , Bossi Alessandra Maria , Cennamo Nunzio
Published: April 2022

Plasmonic bio/chemical sensing based on optical fibers combined with molecularly imprinted nanoparticles (nanoMIPs), which are polymeric receptors prepared by a template-assisted synthesis, has been demonstrated as a powerful method to attain ultra-low detection limits, particularly when exploiting soft nanoMIPs, which are known to deform upon analyte binding. This work presents the development of a surface plasmon resonance (SPR) sensor in silica light-diffusing fibers (LDFs) functionalized with a specific nanoMIP receptor, entailed for the recognition of the protein human serum transferrin (HTR). Despite their great versatility, to date only SPR-LFDs functionalized with antibodies have been reported. Here, the innovative combination of an SPR-LFD platform and nanoMIPs led to the development of a sensor with an ultra-low limit of detection (LOD), equal to about 4 fM, and selective for its target analyte HTR. It is worth noting that the SPR-LDF-nanoMIP sensor was mounted within a specially designed 3D-printed holder yielding a measurement cell suitable for a rapid and reliable setup, and easy for the scaling up of the measurements. Moreover, the fabrication process to realize the SPR platform is minimal, requiring only a metal deposition step.
Walnut Oil Reduces Aβ Levels and Increases Neurite Length in a Cellular Model of Early Alzheimer Disease
Authors: Esselun Carsten , Dieter Fabian , Sus Nadine , Frank Jan , Eckert Gunter P.
Published: April 2022

(1) Background: Mitochondria are the cells’ main source of energy. Mitochondrial dysfunction represents a key hallmark of aging and is linked to the development of Alzheimer’s disease (AD). Maintaining mitochondrial function might contribute to healthy aging and the prevention of AD. The Mediterranean diet, including walnuts, seems to prevent age-related neurodegeneration. Walnuts are a rich source of α-linolenic acid (ALA), an essential n3-fatty acid and the precursor for n3-long-chain polyunsaturated fatty acids (n3-PUFA), which might potentially improve mitochondrial function. (2) Methods: We tested whether a lipophilic walnut extract (WE) affects mitochondrial function and other parameters in human SH-SY5Y cells transfected with the neuronal amyloid precursor protein (APP695). Walnut lipids were extracted using a Soxhlet Extraction System and analyzed using GC/MS and HPLC/FD. Adenosine triphosphate (ATP) concentrations were quantified under basal conditions in cell culture, as well as after rotenone-induced stress. Neurite outgrowth was investigated, as well as membrane integrity, cellular reactive oxygen species, cellular peroxidase activity, and citrate synthase activity. Beta-amyloid (Aβ) was quantified using homogenous time-resolved fluorescence. (3) Results: The main constituents of WE are linoleic acid, oleic acid, α-linolenic acid, and γ- and δ-tocopherol. Basal ATP levels following rotenone treatment, as well as citrate synthase activity, were increased after WE treatment. WE significantly increased cellular reactive oxygen species but lowered peroxidase activity. Membrane integrity was not affected. Furthermore, WE treatment reduced Aβ1–40 and stimulated neurite growth. (4) Conclusions: WE might increase ATP production after induction of mitochondrial biogenesis. Decreased Aβ1–40 formation and enhanced ATP levels might enhance neurite growth, making WE a potential agent to enhance neuronal function and to prevent the development of AD. In this sense, WE could be a promising agent for the prevention of AD.
A Comprehensive Analysis of Plasma Cytokines and Metabolites Shows an Association between Galectin-9 and Changes in Peripheral Lymphocyte Subset Percentages Following Coix Seed Consumption
Authors: Suzuki Yoshio , Miyahara Taisei , Jinnouchi Minami , Miura Yoshiki , Taka Hikari , Kaga Naoko , Ohara-Takada Akiko
Published: April 2022

We previously reported that healthy adult males who consumed coix seeds for 1 week demonstrated an increased intestinal abundance of Faecalibacterium prausnitzii and altered peripheral lymphocyte subset percentages. However, the mechanism underlining these effects has not been elucidated. Therefore, cytokines and metabolites in plasma obtained in this study are comprehensively analyzed. A total of 56 cytokines and 52 metabolites in the plasma are quantified. Among them, 14 cytokines and 9 metabolites show significant changes in their levels following coix seed consumption. We examine the relationship between these changes and those in peripheral lymphocyte subset percentages and intestinal abundance of F. prausnitzii, which is also considerably altered following coix seed consumption. The galectin-9 concentration considerably decreased after coix seed consumption, and these changes correlate with those in cytotoxic T cells and pan T cells. Therefore, galectin-9 is possibly involved in the changes in peripheral lymphocyte subset percentages induced by coix seed consumption.
Myristica fragrans Extract Regulates Gut Microbes and Metabolites to Attenuate Hepatic Inflammation and Lipid Metabolism Disorders via the AhR–FAS and NF-κB Signaling Pathways in Mice with Non-Alcoholic Fatty Liver Disease
Authors: Zhao Wenyu , Guo Min , Feng Jun , Gu Zhennan , Zhao Jianxin , Zhang Hao , Wang Gang , Chen Wei
Published: April 2022

Recent studies have shown that non-alcoholic fatty liver disease (NAFLD) is closely related to the gut microbiome. Myristica fragrans is widely used as a traditional seasoning and has a therapeutic effect on gastrointestinal diseases. Although previous studies have shown that M. fragrans extracts have anti-obesity and anti-diabetes effects in mice fed a high-fat diet, few studies have determined the active components or the corresponding mechanism in vivo. In this study, for the first time, an M. fragrans extract (MFE) was shown to be a prebiotic that regulates gut microbes and metabolites in mice fed a high-fat diet. Bioinformatics, network pharmacology, microbiome, and metabolomics analyses were used to analyze the nutrient–target pathway interactions in mice with NAFLD. The National Center for Biotechnology Information Gene Expression Omnibus database was used to analyze NAFLD-related clinical data sets to predict potential targets. The drug database and disease database were then integrated to perform microbiome and metabolomics analyses to predict the target pathways. The concentrations of inflammatory factors in the serum and liver, such as interleukin-6 and tumor necrosis factor-α, were downregulated by MFE. We also found that the hepatic concentrations of low-density lipoprotein cholesterol, total cholesterol, and triglycerides were decreased after MFE treatment. Inhibition of the nuclear factor kappa B (NF-κB) pathway and downregulation of the fatty acid synthase (FAS)-sterol regulatory element-binding protein 1c pathway resulted in the regulation of inflammation and lipid metabolism by activating tryptophan metabolite–mediated aryl hydrocarbon receptors (AhR). In summary, MFE effectively attenuated inflammation and lipid metabolism disorders in mice with NAFLD through the NF-κB and AhR–FAS pathways.
Prevalence of New-Onset Otological Symptoms in Patients with Temporomandibular Disorders
Authors: Aldè Mirko , Didier Henri Albert , Giannì Aldo Bruno , Sessa Fabiola , Borromeo Giulia , Didier Alexandre Henri , Barozzi Stefania , Zanetti Diego , Di Berardino Federica
Published: April 2022

The aim of this retrospective study was to evaluate the prevalence of new-onset otological symptoms and the possible associations between tinnitus and oral parafunctional habits among patients with temporomandibular disorders (TMD) who attended a Craniofacial Pain Outpatient and a Dentistry Clinic. The medical reports and charts of patients who experienced TMD between 1 February 2016 and 31 December 2017 were reviewed, in order to evaluate the prevalence of new-onset aural fullness, vertigo and tinnitus. Tinnitus was also analyzed in more detail to evaluate possible associations with parafunctional habits. A total of 400 patients (301 females, 99 males) met the inclusion criteria, with a median age of 39.6 ± 15.6 years. Overall, new-onset otological symptoms were reported by 304 (76%) subjects with TMD. Among otological symptoms, aural fullness was the most common (n = 133, 33.3%), followed by tinnitus (n = 92, 23%) and vertigo (n = 79, 19.8%). No significant correlations were found between tinnitus and bruxism (p = 0.28), clenching (p = 0.11), nail-biting (p = 0.96), sleeping prone (p = 0.27), chewing gum (p = 0.99) and talking for a long time (p = 0.42). The present study suggests that all patients with TMD should be investigated for new-onset otological symptoms, regardless of oral parafunctional habits. Early diagnosis would allow to plan personalized and appropriate therapeutic and rehabilitative pathways, minimizing the negative impact due to TMD.
Mycobacterium tuberculosis sensu stricto in African Apes, What Is Its True Health Impact?
Authors: Sanchez Carlos R. , Hidalgo-Hermoso Ezequiel
Published: April 2022

Since the Symposium on Mycobacterial Infections of Zoo Animals held at the National Zoological Park, Smithsonian Institution in 1976, our understanding of tuberculosis (TB) in non-domestic animals has greatly expanded. Throughout the past decades, this knowledge has resulted in improved zoo-habitats and facilities design, stricter biosecurity measures, and advanced diagnostic methods, including molecular techniques, that have significantly decreased the number of clinical disease caused by Mycobacterium tuberculosis in apes under human care settings. In the other hand, exponential growth of human populations has led to human encroachment in wildlife habitat which has resulted in increased inter-species contact and recurrent conflict between humans and wild animals. Although it is widely accepted that non-human primates are susceptible to M. tb infection, opinions differ with regard to the susceptibility to develop disease amongst different taxa. Specifically, some authors suggest that African apes are less susceptible to clinical tuberculosis than other species of primates. The aim of this review article is to evaluate the current scientific literature to determine the actual health impact of disease caused by Mycobacterium tuberculosis and more specifically Mycobacterium tuberculosis sensu stricto in African apes. The literature review included literature databases: Web of Science, Pubmed, Scopus, Wiley, Springer and Science direct, without temporal limit and proceedings of annual conferences in the field of wildlife health. Our general inclusion criteria included information about serological, molecular, pathological (macroscopic and/or microscopic), and clinical evidence of TB in African apes; while our, our more stringent inclusion selection criteria required that in addition to a gross pathology, a molecular test confirmed Mycobacterium tuberculosis sensu stricto as the cause of disease or death. We identified eleven reports of tuberculosis in African apes; of those, only four reports met the more stringent selection criteria that confirmed M. tb sensu stricto in six individuals. All reports that confirmed M. tb sensu stricto originated from zoological collections. Our review suggests that there is little evidence of disease or mortality caused by M. tb in the different species of African apes both under human care and free ranging populations. Additional studies are needed in free-ranging, semi-captive populations (sanctuaries) and animals under human care (zoos and rescue centers) to definitely conclude that this mycobacteria has a limited health effect in African ape species.
Study of Antibodies to Influenza Neuraminidase N2
Authors: Desheva Yulia , Petkova Nadezhda , Smolonogina Tatiana , Donina Svetlana , Go Alexey
Published: April 2022

Humoral immunity to influenza neuraminidase (NA) was evaluated among different groups of people including patients with acute influenza infection and healthy people in different age groups using an enzyme linked lectin assay (ELLA). The amino acid composition of NA of seasonal influenza viruses A/Victoria/361/2011(H3N2) and A/Hong Kong/4801/2014(H3N2) differed by 2%, while cross-reacting neuraminidase-inhibiting (NI) antibodies to them in the same serum samples were detected in 10% of cases. Middle-aged patients born from 1977 to 2000 had a high level of hemagglutination-inhibiting (HI) antibodies to A/Hong Kong/4801/2014(H3N2), but almost no NI antibodies, which may indicate that in the case of a change in the hemagglutinin (HA) subtype, this age group will be susceptible to influenza A/H3N2 viruses. Therefore, it could mean there is a need for priority vaccination of this age group with a vaccine against the appropriate strain. It was shown that after intranasal administration of live influenza vaccine (LAIV) for the 2017–2018 season, serum antibody response was not lower compared to that during natural infection. In older people, antibodies to archival A/H2N2 viruses were detected more often than to modern A/H3N2. Since the conversion of antibodies to HA and NA often did not coincide, antibodies to NA can serve as an additional criterion for assessing the immunogenicity of influenza vaccines.
The Potential of Methocinnamox as a Future Treatment for Opioid Use Disorder: A Narrative Review
Authors: Jordan Colleen G. , Kennalley Amy L. , Roberts Alivia L. , Nemes Kaitlyn M. , Dolma Tenzing , Piper Brian J.
Published: April 2022

The opioid epidemic is an ongoing public health crisis, and the United States health system is overwhelmed with increasing numbers of opioid-related overdoses. Methocinnamox (MCAM) is a novel mu opioid receptor antagonist with an extended duration of action. MCAM has potential to reduce the burden of the opioid epidemic by being used as an overdose rescue treatment and a long-term treatment for opioid use disorder (OUD). The currently available treatments for OUD include naloxone, naltrexone, and methadone. These treatments have certain limitations, which include short duration of action, patient non-compliance, and diversion. A narrative review was conducted using PubMed and Google Scholar databases covering the history of the opioid epidemic, pain receptors, current OUD treatments and the novel drug MCAM. MCAM could potentially be used as both a rescue and long-term treatment for opioid misuse. This is due to its pseudo-irreversible antagonism of the mu opioid receptor, abnormally long duration of action of nearly two weeks, and the possibility of using kappa or delta opioid receptor agonists for pain management during OUD treatment. MCAM’s novel pharmacokinetic and pharmacodynamic properties open a new avenue for treating opioid misuse.
Hadronization and Color Transparency
Authors: Gallmeister Kai , Mosel Ulrich
Published: April 2022

In this paper, the earlier studies by us on the production of hadrons in a nuclear environment are reviewed. A string-breaking model for the initial production of hadrons and a quantum-kinetic Giessen-Boltzmann-Uehling-Uhlenbeck (GiBUU) transport model are used to describe the final state interactions of the newly formed (pre)hadrons. The latter are determined both by the formation times and by the time-development of the hadron–hadron cross section. First, it is shown that only a linear time dependence is able to describe the available hadronizatin data. Then, the results are compared with detailed data from HERMES and Jefferson Laboratory (JLAB) experiments; a rather good agreement is reached for all reactions, studied without any tuning of parameters. Predictions of spectra for pions and kaons for JLAB experiments at 12 GeV are also repeated. Finally, the absence of color transparency (CT) effects in the recent experiment on proton transparencies in quasi-elastic (QE) scattering events on nuclei is discussed. We propose to look instead for CT effects on protons in semi-inclusive deep inelastic scattering (SIDIS) events.
New Insights into the Genomic Structure of Avena L.: Comparison of the Divergence of A-Genome and One C-Genome Oat Species
Authors: Gnutikov Alexander A. , Nosov Nikolai N. , Loskutov Igor G. , Blinova Elena V. , Shneyer Viktoria S. , Probatova Nina S. , Rodionov Alexander V.
Published: April 2022

We used next-generation sequencing analysis of the 3′-part of 18S rDNA, ITS1, and a 5′-part of the 5.8S rDNA region to understand genetic variation among seven diploid A-genome Avena species. We used 4–49 accessions per species that represented the As genome (A. atlantica, A. hirtula, and wiestii), Ac genome (A. canariensis), Ad genome (A. damascena), Al genome (A. longiglumis), and Ap genome (A. prostrata). We also took into our analysis one C-genome species, A. clauda, which previously was found to be related to A-genome species. The sequences of 169 accessions revealed 156 haplotypes of which seven haplotypes were shared by two to five species. We found 16 ribotypes that consisted of a unique sequence with a characteristic pattern of single nucleotide polymorphisms and deletions. The number of ribotypes per species varied from one in A. longiglumis to four in A. wiestii. Although most ribotypes were species-specific, we found two ribotypes shared by three species (one for A. damascena, A. hirtula, and A. wiestii, and the second for A. longiglumis, A. atlantica, and A. wiestii), and a third ribotype shared between A. atlantica and A. wiestii. A characteristic feature of the A. clauda ribotype, a diploid C-genome species, is that two different families of ribotypes have been found in this species. Some of these ribotypes are characteristic of Cc-genome species, whereas others are closely related to As-genome ribotypes. This means that A. clauda can be a hybrid between As- and C-genome oats.
Stochastic Variation in DNA Methylation Modulates Nucleosome Occupancy and Alternative Splicing in Arabidopsis thaliana
Authors: Jabre Ibtissam , Chaudhary Saurabh , Wilson Cornelia M. , Staiger Dorothee , Syed Naeem
Published: April 2022

Plants use complex gene regulatory mechanisms to overcome diverse environmental challenges. For instance, cold stress induces rapid and massive transcriptome changes via alternative splicing (AS) to confer cold tolerance in plants. In mammals, mounting evidence suggests chromatin structure can regulate co-transcriptional AS. Recent evidence also supports co-transcriptional regulation of AS in plants, but how dynamic changes in DNA methylation and the chromatin structure influence the AS process upon cold stress remains poorly understood. In this study, we used the DNA methylation inhibitor 5-Aza-2′-Deoxycytidine (5-aza-dC) to investigate the role of stochastic variations in DNA methylation and nucleosome occupancy in modulating cold-induced AS, in Arabidopsis thaliana (Arabidopsis). Our results demonstrate that 5-aza-dC derived stochastic hypomethylation modulates nucleosome occupancy and AS profiles of genes implicated in RNA metabolism, plant hormone signal transduction, and of cold-related genes in response to cold stress. We also demonstrate that cold-induced remodelling of DNA methylation regulates genes involved in amino acid metabolism. Collectively, we demonstrate that sudden changes in DNA methylation via drug treatment can influence nucleosome occupancy levels and modulate AS in a temperature-dependent manner to regulate plant metabolism and physiological stress adaptation.
Advancement and New Trends in Analysis of Pesticide Residues in Food: A Comprehensive Review
Authors: Wahab Shadma , Muzammil Khursheed , Nasir Nazim , Khan Mohammad Suhail , Ahmad Md Faruque , Khalid Mohammad , Ahmad Wasim , Dawria Adam , Reddy Lingala Kalyan Viswanath , Busayli Abdulrahman Mohammed
Published: April 2022

Food safety is a rising challenge worldwide due to the expanding population and the need to produce food to feed the growing population. At the same time, pesticide residues found in high concentrations in fresh agriculture pose a significant threat to food safety. Presently, crop output is being increased by applying herbicides, fungicides, insecticides, pesticides, fertilizers, nematicides, and soil amendments. A combination of factors, including bioaccumulation, widespread usage, selective toxicity, and stability, make pesticides among the most toxic compounds polluting the environment. They are especially harmful in vegetables and fruits because people are exposed to them. Thus, it is critical to monitor pesticide levels in fruits and vegetables using all analytical techniques available. Any evaluation of the condition of pesticide contamination in fruits and vegetables necessitates knowledge of maximum residue levels (MRLs). We set out the problems in determining various types of pesticides in vegetables and fruits, including the complexity and the diversity of matrices in biological materials. This review examines the different analytical techniques to determine the target analytes that must be isolated before final consumption. Many processes involved determining pesticide residues in fruits and vegetables and their advantages and disadvantages have been discussed with recommendations. Furthermore, MRLs of target pesticide residues in fruit and vegetable samples are discussed in the context of data from the literature. The review also examines MRLs’ impact on the international trade of fruits and vegetables. Accurate, sensitive, and robust analytical procedures are critical to ensuring that pesticide levels in food products are effectively regulated. Despite advances in detection technology, effective sample preparation procedures for pesticide residue measurement in cereals and feedstuffs are still needed. In addition, these methods must be compatible with current analytical techniques. Multi-residue approaches that cover a wide range of pesticides are desired, even though pesticides’ diverse natures, classes, and physio-chemical characteristics make such methods challenging to assemble. This review will be valuable to food analysts and regulatory authorities to monitor the quality and safety of fresh food products.
Immobilization of Collagen on the Surface of a PEEK Implant with Monolayer Nanopores
Authors: Kim Hun , Lee Yang Ho , Kim Nam Kwon , Kang Inn Kyu
Published: April 2022

Polyetheretherketone (PEEK) is the only polymer material that can replace titanium implants in the field of orthopedics. This is because the mechanical properties of PEEK are similar to those of bone, and PEEK has natural radiolucency, chemical stability, and sterilization resistance. Despite these advantages, PEEK has a disadvantage—that it is bio-inert. Therefore, many studies have attempted to change the bio-inertness of PEEK into bioactivity. Among them, a method of forming pores by acid treatment is attracting attention. In this study, an attempt was made to form pores on the surface of PEEK implant using a mixed acid of sulfuric acid and nitric acid. As a result, it was found that the condition when the PEEK surface is in contact with the acid is very important. That is, it was possible to form single-layered nanopores on the surface by contacting PEEK with a mixed acid under ultrasound. Additionally, by immobilizing type I collagen on the porous PEEK surface through dopamine coating, it was possible to obtain collagen-immobilized porous PEEK (P-PEEK-Col) with high compatibility with osteoblasts. This P-PEEK-Col has high potential for use as a bone substitute that promotes bone formation.
Current Options in the Valorisation of Vine Pruning Residue for the Production of Biofuels, Biopolymers, Antioxidants, and Bio-Composites following the Concept of Biorefinery: A Review
Authors: Jesus Meirielly , Romaní Aloia , Mata Fernando , Domingues Lucília
Published: April 2022

Europe is considered the largest producer of wine worldwide, showing a high market potential. Several wastes are generated at the different stages of the wine production process, namely, vine pruning, stalks, and grape marc. Typically, these residues are not used and are commonly discarded. Portugal generates annually approximately 178 thousand metric tons of wine production waste. In this context, the interest in redirecting the use of these residues has increased due to overproduction, great availability, and low costs. The utilization of these lignocellulosic biomasses derived from the wine industry would economically benefit the producers, while mitigating impacts on the environment. These by-products can be submitted to pre-treatments (physical, chemical, and biological) for the separation of different compounds with high industrial interest, reducing the waste of agro-industrial activities and increasing industrial profitability. Particularly, vine-pruning residue, besides being a source of sugar, has high nutritional value and may serve as a source of phenolic compounds. These compounds can be obtained by bioconversion, following a concept of biorefinery. In this framework, the current routes of the valorisation of the pruning residues will be addressed and put into a circular economy context.
Urinary Stent Development and Evaluation Models: In Vitro, Ex Vivo and In Vivo—A European Network of Multidisciplinary Research to Improve Urinary Stents (ENIUS) Initiative
Authors: Buchholz Noor , Budia Alberto , Cruz Julia de la , Kram Wolfgang , Humphreys Owen , Reches Meital , Valero Boix Raquel , Soria Federico
Published: April 2022

Background: When trying to modify urinary stents, certain pre-clinical steps have to be followed before clinical evaluation in humans. Usually, the process starts as an in silico assessment. The urinary tract is a highly complex, dynamic and variable environment, which makes a computer simulation closely reflecting physiological conditions extremely challenging. Therefore, the pre-clinical evaluation needs to go through further steps of in vitro, ex vivo and in vivo assessments. Methods and materials: Within the European Network of Multidisciplinary Research to Improve Urinary Stents (ENIUS), the authors summarized and evaluated stent assessment models in silico, in vitro, ex vivo and in vivo. The topic and relevant sub-topics were researched in a systematic literature search in Embase, Scope, Web of Science and PubMed. Clinicaltrials.gov was consulted for ongoing trials. Articles were selected systematically according to guidelines with non-relevant, non-complete, and non-English or Spanish language articles excluded. Results: In the first part of this paper, we critically evaluate in vitro stent assessment models used over the last five decades, outlining briefly their strengths and weaknesses. In the second part, we provide a step-by-step guide on what to consider when setting up an ex vivo model for stent evaluation on the example of a biodegradable stent. Lastly, the third part lists and discusses the pros and cons of available animal models for urinary stent evaluation, this being the final step before human trials. Conclusions: We hope that this overview can provide a practical guide and a critical discussion of the experimental pre-clinical evaluation steps needed, which will help interested readers in choosing the right methodology from the start of a stent evaluation process once an in silico assessment has been completed. Only a transparent multidisciplinary approach using the correct methodology will lead to a successful clinical implementation of any new or modified stent.
New Method Based on Direct Analysis in Real-Time Coupled with Time-of-Flight Mass Spectrometry (DART-ToF-MS) for Investigation of the Miscibility of Polymer Blends
Authors: AlShehri Mohammed Mousa , ALOthman Zeid A. , Bedjah Hadj Ahmed Ahmed Yassine , Aouak Taieb
Published: April 2022

The miscibility of a series of binary blends such as polystyrene/poly(methyl methacrylate) (PS/PMMA), polystyrene/poly(vinyl chloride)(PS/PVC), poly(vinyl chloride)/poly(polymethyl methacrylate)(PVC/PMMA) and poly(ethylene-co-vinyl alcohol)/poly(lactide-co-glycolide acid) PEVAL/PLGA with equal ratios and poly(ethylene oxide)/poly(hydroxyl propyl methyl cellulose) (PEO/PHPMC) containing 30 and 70 wt% PEO, which were randomly chosen among the widely systems reported in the literature, was investigated by a new method based on a direct analysis in real-time coupled with time-of-flight mass spectrometry (DART-ToF-MS). To reach this goal these pairs of polymers and copolymers were prepared by solvent casting method. As a first step, the DSC technique was undertaken in this work to highlight the published results on the miscibility of these binary systems. The thermogravimetry analysis (TGA) was used to define the optimum decomposition temperature of these blends programmed for the study of miscibility using the DART-ToF-MS technique. The results obtained by this method based on the comparison of the nature of the fragments resulting from the isothermal decomposition of the blend with those of their pure components have been very effective in demonstrating the character of miscibility of these systems. Indeed, it was found that the PS/PMMA-50 and PS/PVC-50 blends were immiscible, PVC/PMMA-50 and PEVAL/PLGA-50 miscible, and the PEO/PHMC partially miscible. This method, which is rapid and uses a very small amount of sample (1–2 mg) can be extended in its application to other blends whose other methods used have shown their limits due to the intrinsic properties of the polymers involved.
Amination of Non-Functional Polyvinyl Chloride Polymer Using Polyethyleneimine for Removal of Phosphorus from Aqueous Solution
Authors: Kim Sok , Park Yun Hwan , Choi Yoon-E
Published: April 2022

The eutrophication of freshwater environments caused by an excess inflow of phosphorus has become a serious environmental issue because it is a crucial factor for the occurrence of harmful algal blooms (HABs) in essential water resources. The adsorptive removal of phosphorus from discharged phosphorus containing effluents has been recognized as one of the most promising solutions in the prevention of eutrophication. In the present study, a polyvinyl chloride (PVC)-polyethyleneimine (PEI) composite fiber (PEI-PVC) was suggested as a stable and recoverable adsorbent for the removal of phosphorus from aqueous phases. The newly introduced amine groups of the PEI-PVC were confirmed by a comparison between the FT-IR and XPS results of the PVC and PEI-PVC. The phosphorus sorption on the PEI-PVC was pH dependent. At the optimum pH for phosphorus adsorption (pH 5), the maximum adsorption capacity of the PEI-PVC fiber was estimated to be 11.2 times higher (19.66 ± 0.82 mg/g) than that of conventional activated carbon (1.75 ± 0.4 mg/g) using the Langmuir isotherm model. The phosphorus adsorption equilibrium of the PEI-PVC was reached within 30 min at pH 5. From the phosphorus-loaded PEI-PVC, 97.4% of the adsorbed amount of phosphorus on the PEI-PVC could be recovered by employing a desorption process using 1M HCl solution without sorbent destruction. The regenerated PEI-PVC through the desorption process maintained a phosphorus sorption capacity almost equal to that of the first use. In addition, consistently with the PVC fiber, the PEI-PVC fiber did not elute any toxic chlorines into the solution during light irradiation. Based on these results, the PEI-PVC fiber can be suggested as a feasible and stable adsorbent for phosphorus removal.
Analysis and Advances in Additive Manufacturing as a New Technology to Make Polymer Injection Molds for World-Class Production Systems
Authors: Lozano Adrian Benitez , Álvarez Santiago Henao , Isaza Carlos Vargas , Montealegre-Rubio Wilfredo
Published: April 2022

The currently growing demand for metallic and polymeric products has undoubtedly changed the rules of manufacturing, enabling customers to more functionally define their products based on their needs. Nowadays, a new technique for rapid tooling, Additive Manufacturing (AM), can create customized products with more complex geometries and short life cycles (flexibility) in order to keep up with the new variables imposed by the manufacturing environment. In the last two decades, the migration from subtractive manufacturing to AM has materialized such products with reduced costs and cycle times. AM has been recently promoted to develop polymer molds for product manufacturing. This paper reviews the main findings in the literature concerning polymer molds created by AM compared to conventional (metal) molds obtained by subtractive manufacturing. Information about specific topics is scarce or nonexistent, for example, about the characterization of the most commonly injected materials and molds used in this type of technology, their mechanical properties (part and mold), designs for all types of geometries, and costs. These aspects are addressed in this literature review, highlighting the advantages of this alternative manufacturing process, which is considered a desirable technology worldwide.
Aluminum Diethylphosphinate-Incorporated Flame-Retardant Polyacrylonitrile Separators for Safety of Lithium-Ion Batteries
Authors: Kang Seok Hyeon , Jeong Hwan Yeop , Kim Tae Ho , Lee Jang Yong , Hong Sung Kwon , Hong Young Taik , Choi Jaewon , So Soonyong , Yoon Sang Jun , Yu Duk Man
Published: April 2022

Herein, we developed polyacrylonitrile (PAN)-based nanoporous composite membranes incorporating aluminum diethylphosphinate (ADEP) for use as a heat-resistant and flame-retardant separator in high-performance and safe lithium-ion batteries (LIBs). ADEP is phosphorus-rich, thermally stable, and flame retardant, and it can effectively suppress the combustibility of PAN nanofibers. Nanofibrous membranes were obtained by electrospinning, and the content of ADEP varied from 0 to 20 wt%. From the vertical burning test, it was demonstrated that the flame retardancy of the composite membranes was enhanced when more than 5 wt% of ADEP was added to PAN, potentially increasing the safety level of LIBs. Moreover, the composite membrane showed higher ionic conductivity and electrolyte uptake (0.83 mS/cm and 137%) compared to those of commercial polypropylene (PP) membranes (Celgard 2400: 0.65 mS/cm and 63%), resulting from interconnected pores and the polar chemical composition in the composite membranes. In terms of battery performance, the composite membrane showed highly stable electrochemical and heat-resistant properties, including superior discharge capacity when compared to Celgard 2400, indicating that the PAN/ADEP composite membrane has the potential to be used as a heat-resistant and flame-retardant separator for safe and high-power LIBs.
The Imperative Need of Metal Salt for the Treatment of Industrial Wastewater via the Synergic Coagulation-Flocculation Method
Authors: Al-Anzi Bader S. , Naik Mehraj-ud-din , Ahmad Mudasir
Published: April 2022

Tile industry wastewater is known to contain a high concentration of TSS and turbidity resulting from various raw materials. In the present study, the effectiveness of the coagulation process on turbidity and TSS removal from Kuwait ceramic tile industry wastewater was investigated using ferric chloride as a coagulant. The experiments were conducted using jar tests to determine the optimum operating conditions of coagulant dosages, pH, and settling time. It was found that the coagulant dosage and medium pH greatly affect the efficiency of the coagulation process. A gradual increase in coagulant dosage from 10 to 50 mg/L increased the efficiency of turbidity removal from 95.6% to 99.5%. The efficiency of the coagulation process was also found to be dependent on pH values, where higher pH improved the efficiency of turbidity removal. It was found that a medium pH of 10, 1 h settling time, and 50 mg/L of coagulant dosage are the optimum process conditions to achieve almost complete removal of turbidity (99.5%) and TSS (99.8%). This study concluded that coagulation might be useful as a primary wastewater treatment process for tile industry wastewater.
Application of Quantum Cognition to Judgments for Medical Decisions
Authors: Yi Sheng , Lu Meijuan , Busemeyer Jerome
Published: April 2022

The psychology of judgment and decision making can provide useful guidance to the task of medical decision making. More specifically, we describe how a new approach to judgment and decisions, based on quantum probability theory, can shed new light on seemingly irrational judgments, as well as indicate ways to ameliorate these judgment errors. Five different types of probability judgment errors that occur in medical decisions are reviewed. For each one, we provide a simple account using theory from quantum cognition. We conclude by drawing the implications of quantum cognition for ameliorating these common medical probability judgment errors.
Growth Development, Physiological Status and Water Footprint Assessment of Nursery Young Olive Trees (Olea europaea L. ‘Konservolea’) Irrigated with Urban Treated Wastewater
Authors: Fotia Konstantina , Nanos George , Barouchas Pantelis , Giannelos Markos , Linardi Aikaterini , Vallianatou Aikaterini , Mpeza Paraskevi , Tsirogiannis Ioannis
Published: April 2022

Application of urban treated wastewater (TWW) has been practiced globally as an alternative irrigation water source in areas where access to safe and abundant freshwater is limited. Water footprint (WF) has been employed over the last decades as a tool for the assessment of the sustainable management of water resources. In the present study, the suitability of TWW for the irrigation of nursery young olive trees (Olea europaea L. ‘Konservolea’), one of the main table olive cultivars in Greece, the second global table olive exporter, was tested and compared to tap water irrigation and application of zeolite on soil. Plant growth and physiological parameters and stress indicators were measured. Additionally, a WF assessment was performed, distinguishing TWW from freshwater (blue water) resources in order to examine the possibility of minimizing the environmental impact through the limitation of freshwater use. Plants irrigated with TWW performed better in most of the growth and physiological parameters measured compared to the other treatments. Stress indicators revealed that TWW did not induce any additional stress. TWW could be used as an irrigation water source for young olive trees for at least a short period during their growth as a safe and sustainable alternate of blue water resources. Additionally, the WF assessment showed that the application of TWW could be a significant blue water saving measure.
On the Evaluation of Both Spatial and Temporal Performance of Distributed Hydrological Models Using Remote Sensing Products
Authors: Nguyen Tam V. , Uniyal Bhumika , Tran Dang An , Pham Thi Bich Thuc
Published: April 2022

Evaluating the spatial and temporal model performance of distributed hydrological models is necessary to ensure that the simulated spatial and temporal patterns are meaningful. In recent years, spatial and temporal remote sensing data have been increasingly used for model performance evaluation. Previous studies, however, have focused on either the temporal or spatial model performance evaluation. In addition, temporal (or spatial) model performance evaluation is often conducted in a spatially (or temporally) lumped approach. Here, we evaluated (1) the temporal model performance evaluation in a spatially distributed approach (spatiotemporal) and (2) the spatial model performance in a temporally distributed approach (temporospatial). We further demonstrated that both spatiotemporal and temporospatial model performance evaluations are necessary since they provide different aspects of the model performance. For this, a case study was developed using the Soil and Water Assessment Tool (SWAT) for the Upper Baitarani catchment in India, and the spatiotemporal and temporospatial model performance was evaluated against three different remotely based actual evapotranspiration (ETa) products (MOD16 A2, SSEBop, and TerraClimate). The results showed that an increase in the spatiotemporal model performance would not necessarily lead to an increase in the temporospatial model performance and vice versa, depending on the evaluation statistics. Overall, this study has highlighted the necessity of a joint spatiotemporal and temporospatial model performance evaluation to understand/improve spatial and temporal model behavior/performance.
Analyzing the Error Pattern of InSAR-Based Mining Subsidence Estimation Caused by Neglecting Horizontal Movements
Authors: Ma Zelin , Yang Zefa , Xing Xuemin
Published: April 2022

It is common to estimate underground mining-induced subsidence from interferometric synthetic aperture radar (InSAR) displacement observations by Neglecting hOrizontal moVements (NOV). Such a strategy would cause large errors in the NOV-estimated subsidence. This issue was proven and the theoretical equation of the resulting errors has been deduced before. However, the systematic analysis of the error pattern (e.g., spatial distribution) and its relationship between some critical influence factors (e.g., lithology of overlying rock strata) is lacking to date. To circumvent this, a method was first presented to assess the errors of the NOV-estimated mining subsidence in this study. Then, the error pattern and the influence factors of the NOV-estimated mining subsidence were discussed. The results suggest that the errors of the NOV-estimated mining subsidence spatially follow a “peak-to-valley” shape, with an absolute “peak-to-valley angle” of 5–15°. In addition, for the same underground mining geometry, the error magnitudes of the NOV-estimated mining subsidence under hard lithology of overlying rock strata are smaller than those under soft lithology, and vice versa. These results would be beneficial to guide the scientific use of the NOV method for understanding the deformation mechanism and controlling the geohazards associated with underground mining and other similar anthropogenic activities.
A Characteristics Set Computation Model for Internal Wavenumber Spectra and Its Validation with MODIS Retrieved Parameters in the Sulu Sea and Celebes Sea
Authors: Yang Yongzeng , Sun Meng , Sun Lina , Xia Changshui , Teng Yong , Cui Xinmei
Published: April 2022

The quasi-linear or nonlinear interactions among different ocean motions dominate the system internal structure and appearance feature presented in spatial and temporal evolution. However, deficiency of the characteristics set computation model for internal wavenumber spectra proves to be a serious barrier to derive interaction mechanisms of internal waves with large or small scale ocean motions. In this study, a characteristics set computation model for internal wavenumber spectra is proposed for complicated offshore environments. The refraction of current shear instability, bottom topography and the reflection at surface and bottom are attentively considered in the complicated characteristics inlaid scheme. Model results are validated with MODIS retrieved internal wave parameters in the Sulu Sea and Celebes Sea. This original characteristics set computation model for internal wavenumber spectra can be used widely and can further improve the understandings of generation, dissipation, nonlinear wave-wave interaction and mixing process of internal waves.
SHAP-Based Interpretable Object Detection Method for Satellite Imagery
Authors: Kawauchi Hiroki , Fuse Takashi
Published: April 2022

There is a growing need for algorithms to automatically detect objects in satellite images. Object detection algorithms using deep learning have demonstrated a significant improvement in object detection performance. However, deep-learning models have difficulty in interpreting the features for inference. This difficulty is practically problematic when analyzing earth-observation images, which are often used as evidence for public decision-making. In addition, for the same reason, it is difficult to set an explicit policy or criteria to improve the models. To deal with these challenges, we introduce a feature attribution method that defines an approximate model and calculates the attribution of input features to the output of a deep-learning model. For the object detection models of satellite images with complex textures, we propose a method to visualize the basis of inference using pixel-wise feature attribution. Furthermore, we propose new methods for model evaluation, regularization, and data selection, based on feature attribution. Experimental results demonstrate the feasibility of the proposed methods for basis visualization and model evaluation. Moreover, the results illustrate that the model using the proposed regularization method can avoid over-fitting and achieve higher performance, and the proposed data selection method allows for the efficient selection of new training data.
Numerical Simulation and Experimental Study of the Drop Impact for a Multiphase System Formed by Two Immiscible Fluids
Authors: Sochan Agata , Lamorski Krzysztof , Bieganowski Andrzej
Published: April 2022

The multiphase splash phenomenon is especially interesting in the context of environmental protection, as it could be a mechanism for transporting various types of pollution. A numerical 3D multiphase transport model was applied to a splash that occurred under the impact of a petrol drop on the water surface. The splash phenomenon in immiscible liquids was simulated using the multiphaseInterFoam solver, i.e., a part of the OpenFOAM computational fluid dynamics software implementing the finite volume method (FVM) for space discretization. Thirteen variants with a variable drop size (3.00–3.60 mm) or drop velocity (3.29–3.44 m/s) were conducted and validated experimentally based on splash images taken by a high-speed camera (2800 fps). Based on the numerical simulation, it was possible to analyse aspects that were difficult or impossible to achieve experimentally due to the limitations of the image analysis method. The aspects included the cavity spread, the jet forming moment, and, notably, the scale of the petroleum contamination spread in the splash effect. The simulations showed that droplets detaching from the crown did not consist of pure water but were mostly a “mixture” of water and petrol or petrol alone. The applied modelling workflow is an efficient way to simulate three-phase splash phenomena.