bluuebunny/crossref_metadata_2025 huggingface dataset:
Environmental Challenges in the Residential Sector: Life Cycle Assessment of Mexican Social Housing
Authors: Gámez-García Diana Carolina , Saldaña-Márquez Héctor , Gómez-Soberón José Manuel , Arredondo-Rea Susana Paola , Gómez-Soberón María Consolación , Corral-Higuera Ramón
Published: July 2019

Social Housing (SH) in Mexico has a potentially important role in reducing both the emission of greenhouse gases and the use of non-renewable resources, two of the main challenges facing not only Mexico but the planet as a whole. This work assesses the environmental impact generated by the embodied stages of a typical SH throughout its life cycle (cradle to grave), by means of a Life Cycle Assessment (LCA). Two types of envelope and interior walls and three types of windows are compared. It was found that SH emits 309 kg CO2 eq/m2 and consumes 3911 MJ eq/m2 in the product stages (A1 to A3) and construction process (A4 to A5); the most important stages are those referring to the products, namely, A1 to A3, B4 (replacement) and B2 (maintenance). Additionally, benefits were found in the use of lightweight and thermal materials, such as concrete blocks lightened with pumice or windows made of PVC or wood. Although the use of LCA is incipient in the housing and construction sector in Mexico, this work shows how its application is not only feasible but recommended as it may become a basic tool in the search for sustainability.
Power Production Estimates from Geothermal Resources by Means of Small-Size Compact Climeon Heat Power Converters: Case Studies from Portugal (Sete Cidades, Azores and Longroiva Spa, Mainland)
Authors: Trota António , Ferreira Pedro , Gomes Luis , Cabral João , Kallberg Peter
Published: July 2019

Renewable forms of energy are increasingly penetrating the electricity market, particularly, geothermal energy. A wide range of resource temperatures and fluid quality are converted mostly using traditional binary power plants and, recently, using Climeon modular units. Portuguese natural geothermal resources are far from precise estimations. Despite the parameter uncertainties, electric power resource estimations of two natural geothermal reservoirs are presented: a volcanic sourced heated high-enthalpy geothermal reservoir in Sete Cidades, São Miguel Island, Azores; and a low-enthalpy geothermal reservoir linked to a fractured zone in a granitic setting in Longroiva, in the northern part of the Portuguese mainland. Based on the volumetric method, we assessed the power potential of geothermal resources in Sete Cidades and Longroiva using a probabilistic methodology—Monte Carlo simulation. The average reserve estimations for Climeon module were 5.66 MWe and 0.64 MWe for Sete Cidades and Longroiva, respectively. This figure was by far higher when compared to traditional binary technology; those differences were mostly attributed to distinct conversions efficiency factors.
The Influence of Selected Plant Essential Oils on Morphological and Physiological Characteristics in Pseudomonas Orientalis
Authors: Leja Katarzyna , Szudera-Kończal Kamila , Świtała Ewa , Juzwa Wojciech , Kowalczewski Przemysław , Czaczyk Katarzyna
Published: July 2019

The aim of this work was to estimate the antibacterial activity of selected essential oils on Pseudomonas orientalis strains isolated from foods. An attempt was also made to identify the mechanisms of the action of the plant oils. Classical methods of assessment of the effectiveness of antimicrobial activity of oils were linked with flow cytometry. It was observed that bergamot, lemongrass, bitter orange, juniper, and black pepper oils have bacteriostatic effect against P. orientalis P49. P. orientalis P110 is sensitive to lime, lemongrass, juniper, rosemary, and black pepper oils. Additionally, plant oils with biostatic effect on P. orientalis limited the intracellular metabolic activity of cells; this was closely linked with the ability of plant oils’ bioactive components to interact with bacteria cell membrane, causing the release of membrane proteins. As a result, the selective permeability of the cell membranes were damaged and the bacterial shape was transformed to coccoid in form.
Climate and Cultural Heritage: The Case Study of “Real Sito di Carditello”
Authors: Spagnuolo Antonio , Vetromile Carmela , Masiello Antonio , Alberghina Maria Francesca , Schiavone Salvatore , Lubritto Carmine
Published: July 2019

A monitoring campaign performed on the Real Site of Carditello (found in the commune of San Tammaro, province of Caserta, Italy) was aimed to evaluate the parameters related to air quality and then to control and prevent the degradation risk. The analyses were carried out in the context of the project “Innovative methods and technologies for the conservation, valorisation and use of cultural heritage: environmental and archaeological analyses”, within the CULTURA CREA-MIBAC program, which aims to provide innovative methods for conservation and enhancement of cultural heritage through environmental and archaeometric analyses. Preliminarily, using field equipment, non-destructive investigations were carried out to identify constituent, restoration, and/or degradation materials belonging to the wall paintings on the main floor and to assess their conservation state under the current environmental conditions. Environmental monitoring was performed both outdoor (to assess the effects of atmospheric pollution and changes in the climate of the structure) and indoor the rooms of the site (to evaluate the conditions of conservation and fruition). The collected data are reported in a web platform, where one can check the real time monitoring data. The platform integrates both environmental data and information regarding the "health status" of site in order to provide a detailed analysis useful for its protection.
The Main Drivers of Wetland Changes in the Beijing-Tianjin-Hebei Region
Authors: Zhang Liyun , Zhen Quan , Cheng Min , Ouyang Zhiyun
Published: July 2019

Wetlands are the most threatened ecosystem in China, and wetland conservation is a national priority because of their importance for water security, flood mitigation, and biodiversity conservation. A goal has been established for the Beijing-Tianjin-Hebei Region (BTH) to recover 340 km2 of wetlands by 2020. To guide restoration and protection efforts, policymakers need information on the trends of wetland loss, conversion of wetlands, and their associated human drivers. The main drivers of changes in different wetland types in the BTH were identified and quantified from 2000 to 2015. In 2015, there was 6264.07 km2 less wetland area than in 2000, with the remaining wetlands primarily located in Hebei and Tianjin. Reservoirs/ponds were the most abundant wetland type, followed by herbaceous swamps, rivers, canals and channels, and then lakes as the least represented. There were continuous losses of wetlands from 2000 to 2015, with marked decreases for rivers (30.48%), channels/canals (23.30%), and herbaceous swamps (16.12%). However, there was an increase in the area of lakes and reservoirs/ponds, with increases of 54.96% and 3.47%, respectively. The largest changes in natural wetlands were due to agricultural production followed by artificialization and grassland expansion. The driving forces of the observed changes were specific to each local region. According to an aggregated boosted trees (ABT) analysis, gross farm production, total aquatic products, and irrigated area were the top three drivers of the decrease in natural wetlands, which agreed with the main patterns of change in the BTH. The purpose of this study was to provide guidance for policy makers working to meet the 2020 BTH wetland recovery target. Recommendations were provided at the provincial level, including water transfers across provincial boundaries, the control of agricultural expansion, exploration of species-specific irrigation deficits, a reduction in the artificialization of land surfaces, the development of a sustainable intensified aquaculture model, and the promotion of awareness of wetland importance among local people.
Attention Restoration Space on a University Campus: Exploring Restorative Campus Design Based on Environmental Preferences of Students
Authors: Lu Ming , Fu Jingwan
Published: July 2019

Students studying for a long time frequently suffer from attentional fatigue; however, campuses lack specific spaces in which to restore attention. This study aimed to explore the significant perceptual factors related to student selection of landscape types that they perceive as most relaxing on a university campus. To understand the design factors of an attention restoration space, this study examined the preference of students regarding restorative environments on university campuses at six universities in northeastern China using a questionnaire survey (n = 360). Place-mapping revealed the spatial characteristics of the preferences of students for relaxing in the available space. The primary perceptual factors were obtained using correlation analysis and keyword frequency. A relationship model of landscape types and perceptual factors was established using categorical regression (CATREG). Results showed that waterfront spaces have the optimal perceived attention restoration effect, followed by vegetation spaces, courtyard spaces and square spaces. Visibility, accessibility, comfort, recognition and sense of belonging are significant perceptual factors that should be first considered. Moreover, the optimal selection of design factors depends on the interaction of landscape types and perceptual factors. The design implications may assist designers to gain a new perspective on student requirements for a healthy environment.
Regionalization Analysis and Mapping for the Source and Sink of Tourist Flows
Authors: Gu Qiushi , Zhang Haiping , Chen Min , Chen Chongcheng
Published: July 2019

At present, population mobility for the purpose of tourism has become a popular phenomenon. As it becomes easier to capture big data on the tourist digital footprint, it is possible to analyze the respective regional features and driving forces for both tourism sources and destination regions at a macro level. Based on the data of tourist flows to Nanjing on five short-period national holidays in China, this study first calculated the travel rate of tourist source regions (315 cities) and the geographical concentration index of the visited attractions (51 scenic spots). Then, the spatial autocorrelation metrics index was used to analyze the global autocorrelation of the travel rates of tourist source regions and the geographical concentration index of the tourist destinations on five short-term national holidays. Finally, a heuristic unsupervised machine-learning method was used to analyze and map tourist sources and visited attractions by adopting the travel rate and the geographical concentration index accordingly as regionalized variables. The results indicate that both source and sink regions expressed distinctive regional differentiation patterns in the corresponding regional variables. This study method provides a practical tool for analyzing regionalization of big data in tourist flows, and it can also be applied to other origin-destination (OD) studies.
Polymorphism in the Promoter Region of NFE2L2 Gene Is a Genetic Marker of Susceptibility to Cirrhosis Associated with Alcohol Abuse
Authors: Nunes dos Santos Kemper , Florentino Rodrigo M. , França Andressa , Lima Filho Antônio Carlos Melo , Santos Marcone Loiola dos , Missiaggia Dabny , Fonseca Matheus de Castro , Brasil Costa Igor , Vidigal Paula Vieira Teixeira , Nathanson Michael H. , Lemos Fernanda de Oliveira , Leite M. Fatima
Published: July 2019

Alcoholic liver disease (ALD) is a highly prevalent spectrum of pathologies caused by alcohol overconsumption. Morbidity and mortality related to ALD are increasing worldwide, thereby demanding strategies for early diagnosis and detection of ALD predisposition. A potential candidate as a marker for ALD susceptibility is the transcription factor nuclear factor erythroid-related factor 2 (Nrf2), codified by the nuclear factor erythroid 2-related factor 2 gene (NFE2L2). Nrf2 regulates expression of proteins that protect against oxidative stress and inflammation caused by alcohol overconsumption. Here, we assessed genetic variants of NFE2L2 for association with ALD. Specimens from patients diagnosed with cirrhosis caused by ALD were genotyped for three NFE2L2 single nucleotide polymorphisms (SNP) (SNPs: rs35652124, rs4893819, and rs6721961). Hematoxylin & eosin and immunohistochemistry were performed to determine the inflammatory score and Nrf2 expression, respectively. SNPs rs4893819 and rs6721961 were not specifically associated with ALD, but analysis of SNP rs35652124 suggested that this polymorphism predisposes to ALD. Furthermore, SNP rs35652124 was associated with a lower level of Nrf2 expression. Moreover, liver samples from ALD patients with this polymorphism displayed more severe inflammatory activity. Together, these findings provide evidence that the SNP rs35652124 variation in the Nrf2-encoding gene NFE2L2 is a potential genetic marker for susceptibility to ALD.
Molecular Mechanisms of p63-Mediated Squamous Cancer Pathogenesis
Authors: Moses Michael A. , George Andrea L. , Sakakibara Nozomi , Mahmood Kanwal , Ponnamperuma Roshini M. , King Kathryn E. , Weinberg Wendy C.
Published: July 2019

The p63 gene is a member of the p53/p63/p73 family of transcription factors and plays a critical role in development and homeostasis of squamous epithelium. p63 is transcribed as multiple isoforms; ΔNp63α, the predominant p63 isoform in stratified squamous epithelium, is localized to the basal cells and is overexpressed in squamous cell cancers of multiple organ sites, including skin, head and neck, and lung. Further, p63 is considered a stem cell marker, and within the epidermis, ΔNp63α directs lineage commitment. ΔNp63α has been implicated in numerous processes of skin biology that impact normal epidermal homeostasis and can contribute to squamous cancer pathogenesis by supporting proliferation and survival with roles in blocking terminal differentiation, apoptosis, and senescence, and influencing adhesion and migration. ΔNp63α overexpression may also influence the tissue microenvironment through remodeling of the extracellular matrix and vasculature, as well as by enhancing cytokine and chemokine secretion to recruit pro-inflammatory infiltrate. This review focuses on the role of ΔNp63α in normal epidermal biology and how dysregulation can contribute to cutaneous squamous cancer development, drawing from knowledge also gained by squamous cancers from other organ sites that share p63 overexpression as a defining feature.
Task Assignment Algorithm Based on Trust in Volunteer Computing Platforms
Authors: Xu Ling , Qiao Jianzhong , Lin Shukuan , Qi Ruihua
Published: July 2019

In volunteer computing (VC), the expected availability time and the actual availability time provided by volunteer nodes (VNs) are usually inconsistent. Scheduling tasks with precedence constraints in VC under this situation is a new challenge. In this paper, we propose two novel task assignment algorithms to minimize completion time (makespan) by a flexible task assignment. Firstly, this paper proposes a reliability model, which uses a simple fuzzy model to predict the time interval provided by a VN. This reliability model can reduce inconsistencies between the expected availability time and actual availability time. Secondly, based on the reliability model, this paper proposes an algorithm called EFTT (Earliest Finish Task based on Trust, EFTT), which can minimize makespan. However, EFTT may induce resource waste in task assignment. To make full use of computing resources and reduce task segmentation rate, an algorithm IEFTT (improved earliest finish task based on trust, IEFTT) is further proposed. Finally, experimental results verify the efficiency of the proposed algorithms.
The Role of the Federal Reserve in the U.S. Housing Crisis: A VAR Analysis with Endogenous Structural Breaks
Authors: Barari Mahua , Kundu Srikanta
Published: July 2019

This paper reexamines the role of the Federal Reserve in triggering the recent housing crisis. Specifically, we explore if the relationship between the federal funds rate and the housing variables underwent structural changes in the wake of the housing crisis. Using quarterly data spanning 1960–2017, we estimate a VAR model involving federal funds rate, real GDP growth and a housing variable (captured by house price inflation or residential investment share or housing starts) and conduct time series analysis for the pre- and post-crisis periods. While previous studies mostly set break-dates based on events known a priori to split the full sample to subsamples, we endogenously determine structural break points occurring at multiple unknown dates. Our Granger causality analysis indicates that the federal funds rate did not cause house price inflation, although it caused residential investment share and housing starts in the pre-crisis period. In the post-crisis period, the real GDP growth caused residential investment and housing starts while house price inflation had a momentum of its own. Our impulse response and forecast error variance decomposition analysis reinforce these results. Overall, our findings suggest that housing volume fluctuates more than house prices over the business cycle.
Comparison of Meshing Strategies in THR Finite Element Modelling
Authors: Ruggiero Alessandro , D’Amato Roberto , Affatato Saverio
Published: July 2019

In biomechanics and orthopedics, finite element modelling allows simulating complex problems, and in the last few years, it has been widely used in many applications, also in the field of biomechanics and biotribology. As is known, one crucial point of FEM (finite element model) is the discretization of the physical domain, and this procedure is called meshing. A well-designed mesh is necessary in order to achieve accurate results with an acceptable computational effort. The aim of this work is to test a finite element model to simulate the dry frictionless contact conditions of a hip joint prosthesis (a femoral head against an acetabular cup) in a soft bearing configuration by comparing the performances of 12 common meshing strategies. In the simulations, total deformation of the internal surface of the cup, contact pressure, and the equivalent von Mises stress are evaluated by using loads and kinematic conditions during a typical gait, obtained from a previous work using a musculoskeletal multibody model. Moreover, accounting for appropriate mesh quality metrics, the results are discussed, underlining the best choice we identified after the large amount of numerical simulations performed.
What are the Main Sensor Methods for Quantifying Pesticides in Agricultural Activities? A Review
Authors: Zamora-Sequeira Roy , Starbird-Pérez Ricardo , Rojas-Carillo Oscar , Vargas-Villalobos Seiling
Published: July 2019

In recent years, there has been an increase in pesticide use to improve crop production due to the growth of agricultural activities. Consequently, various pesticides have been present in the environment for an extended period of time. This review presents a general description of recent advances in the development of methods for the quantification of pesticides used in agricultural activities. Current advances focus on improving sensitivity and selectivity through the use of nanomaterials in both sensor assemblies and new biosensors. In this study, we summarize the electrochemical, optical, nano-colorimetric, piezoelectric, chemo-luminescent and fluorescent techniques related to the determination of agricultural pesticides. A brief description of each method and its applications, detection limit, purpose—which is to efficiently determine pesticides—cost and precision are considered. The main crops that are assessed in this study are bananas, although other fruits and vegetables contaminated with pesticides are also mentioned. While many studies have assessed biosensors for the determination of pesticides, the research in this area needs to be expanded to allow for a balance between agricultural activities and environmental protection.
Carbon Nanotube and Cellulose Nanocrystal Hybrid Films
Authors: Jiang Mingzhe , Seney Robert , Bayliss Paul Charles , Kitchens Christopher L.
Published: July 2019

The use of cellulose nanocrystals (CNC) in high performance coatings is attractive for micro-scale structures or device fabrication due to the anisotropic geometry, however CNC are insulating materials. Carbon nanotubes (CNT) are also rod-shaped nanomaterials that display high mechanical strength and electrical conductivity. The hydrophobic regions of surface-modified CNC can interact with hydrophobic CNT and aid in association between the two anisotropic nanomaterials. The long-range electrostatic repulsion of CNC plays a role in forming a stable CNT and CNC mixture dispersion in water, which is integral to forming a uniform hybrid film. At concentrations favorable for film formation, the multiwalled nanotubes + CNC mixture dispersion shows cellular network formation, indicating local phase separation, while the single-walled nanotube + CNC mixture dispersion shows schlieren texture, indicating liquid crystal mixture formation. Conductive CNT + CNC hybrid films (5–20 μm thick) were cast on glass microscope slides with and without shear by blade coating. The CNT + CNC hybrid films electrical conductivity increased with increasing CNT loadings and some anisotropy was observed with the sheared hybrid films, although to a lesser extent than what was anticipated. Percolation models were applied to model the hybrid film conductivity and correlate with the hybrid film microstructure.
Magnetic Polyurea Nano-Capsules Synthesized via Interfacial Polymerization in Inverse Nano-Emulsion
Authors: Natour Suzana , Levi-Zada Anat , Abu-Reziq Raed
Published: July 2019

Polyurea (PU) nano-capsules have received voluminous interest in various fields due to their biocompatibility, high mechanical properties, and surface functionality. By incorporating magnetic nanoparticle (MNPs) into the polyurea system, the attributes of both PU and MNPs can be combined. In this work, we describe a facile and quick method for preparing magnetic polyurea nano-capsules. Encapsulation of ionic liquid-modified magnetite nanoparticles (MNPs), with polyurea nano-capsules (PU NCs) having an average size of 5–20 nm was carried out through interfacial polycondensation between amine and isocyanate monomers in inverse nano-emulsion (water-in-oil). The desired magnetic PU NCs were obtained utilizing toluene and triple-distilled water as continuous and dispersed phases respectively, polymeric non-ionic surfactant cetyl polyethyleneglycol/polypropyleneglycol-10/1 dimethicone (ABIL EM 90), diethylenetriamine, ethylenediamine diphenylmethane-4,4′-diisocyanate, and various percentages of the ionic liquid-modified MNPs. High loading of the ionic liquid-modified MNPs up to 11 wt% with respect to the dispersed aqueous phase was encapsulated. The magnetic PU NCs were probed using various analytical instruments including electron microscopy, infrared spectroscopy, X-ray diffraction, and nuclear magnetic spectroscopy. This unequivocally manifested the successful synthesis of core-shell polyurea nano-capsules even without utilizing osmotic pressure agents, and confirmed the presence of high loading of MNPs in the core.
Diallyl Disulfide Induces Apoptosis and Autophagy in Human Osteosarcoma MG-63 Cells through the PI3K/Akt/mTOR Pathway
Authors: Yue Ziqi , Guan Xin , Chao Rui , Huang Cancan , Li Dongfang , Yang Panpan , Liu Shanshan , Hasegawa Tomoka , Guo Jie , Li Minqi
Published: July 2019

Diallyl disulfide (DADs), a natural organic compound, is extracted from garlic and scallion and has anti-tumor effects against various tumors. This study investigated the anti-tumor activity of DADs in human osteosarcoma cells and the mechanisms. MG-63 cells were exposed to DADs (0, 20, 40, 60, 80, and 100 μM) for different lengths of time (24, 48, and 72 h). The CCK8 assay results showed that DADs inhibited osteosarcoma cell viability in a dose-and time-dependent manner. FITC-Annexin V/propidium iodide staining and flow cytometry demonstrated that the apoptotic ratio increased and the cell cycle was arrested at the G2/M phase as the DADs concentration was increased. A Western blot analysis was employed to detect the levels of caspase-3, Bax, Bcl-2, LC3-II/LC3-I, and p62 as well as suppression of the mTOR pathway. High expression of LC3-II protein revealed that DADs induced formation of autophagosome. Furthermore, DADs-induced apoptosis was weakened after adding 3-methyladenine, demonstrating that the DADs treatment resulted in autophagy-mediated death of MG-63 cells. In addition, DADs depressed p-mTOR kinase activity, and the inhibited PI3K/Akt/mTOR pathway increased DADs-induced apoptosis and autophagy. In conclusion, our results reveal that DADs induced G2/M arrest, apoptosis, and autophagic death of human osteosarcoma cells by inhibiting the PI3K/Akt/mTOR signaling pathway.
Solidago graminifolia L. Salisb. (Asteraceae) as a Valuable Source of Bioactive Polyphenols: HPLC Profile, In Vitro Antioxidant and Antimicrobial Potential
Authors: Toiu Anca , Vlase Laurian , Vodnar Dan Cristian , Gheldiu Ana-Maria , Oniga Ilioara
Published: July 2019

Solidago species are often used in traditional medicine as anti-inflammatory, diuretic, wound-healing and antimicrobial agents. Still, the bioactive compounds and biological activities of some species have not been studied. The present work aimed to investigate the polyphenolic profile and the biological properties of Solidago graminifolia L. Salisb., a poorly explored medicinal plant. The hydroalcoholic extracts from aerial parts were evaluated for total phenolic content (TPC), total flavonoid content (TFC) and the polyphenolic compounds were investigated by HPLC-MS. The antioxidant potential in vitro was determined using DPPH and FRAP assays. Antibacterial and antifungal effects were evaluated by dilution assays and MIC, MBC and MFC were calculated. The results showed that Solidago graminifolia aerial parts contain an important amount of total phenolics (192.69 mg GAE/g) and flavonoids (151.41 mg RE/g), with chlorogenic acid and quercitrin as major constituents. The hydroalcoholic extracts showed promising antioxidant and antimicrobial potential, with potent antibacterial activity against Staphylococcus aureus and important antifungal effect against Candida albicans and C. parapsilosis. The obtained results indicated that the aerial parts of Solidago graminifolia could be used as novel resource of phytochemicals in herbal preparations with antioxidant and antimicrobial activities.
Anti-Diabetic Effect of a Shihunine-Rich Extract of Dendrobium loddigesii on 3T3-L1 Cells and db/db Mice by Up-Regulating AMPK–GLUT4–PPARα
Authors: Li Xue-Wen , Huang Meixiang , Lo Kakei , Chen Wei-Li , He Ying-Yan , Xu Yongli , Zheng Huizhen , Hu Haiyan , Wang Jun
Published: July 2019

The stems of Dendrobium loddigesii, a Chinese herb, are often used to treat diabetes and its polar extract is rich in shihunine, a water-soluble Orchidaceae alkaloid, but little is known about the anti-diabetes effects and mechanism of shihunine. This study investigated the anti-diabetic effect of a shihunine-rich extract of D. loddigesii (DLS) based on 3T3-L1 cells and db/db mice. The underlying mechanisms were primarily explored using Western blot analysis and immunohistochemical staining. The 3T3-L1 cell experiments showed that DLS can reduce the intracellular accumulation of oil droplets as well as triglycerides (p < 0.001) and promote the 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2deoxyglucose (2-NBDG) uptake of 3T3-L1 cells (p < 0.001). The animal experiments confirmed that after 8 weeks of DLS treatment, the body weight, fasting blood sugar, and serum lipid levels of mice were significantly lowered, and the oral glucose tolerance test and serum insulin level were significantly improved compared to the no-treatment diabetes mellitus group. Further histomorphology observation led to the conclusion that the quantities of islet cells were significantly increased and the increase in adipose cell size was significantly suppressed. The immunohistochemical test of pancreatic tissue revealed that DLS inhibited the expression of cleaved cysteine aspartic acid-specific protease 3 (cleaved caspase-3). Western blot experiments showed that DLS had agonistic effects on adenosine monophosphate (AMP)-activated protein kinase phosphorylation (p-AMPK) and increased the expression levels of peroxisome proliferator-activated receptor α (PPARα) and glucose transporter 4 (GLUT4) in liver or adipose tissues. These data suggest that the shihunine-rich extract of D. loddigesii is an anti-diabetic fraction of D. loddigesii. Under our experimental condition, DLS at a dose of 50 mg/kg has good anti-diabetic efficacy.
Discrimination of Natural Mature Acacia Honey Based on Multi-Physicochemical Parameters Combined with Chemometric Analysis
Authors: Ma Tianchen , Zhao Haoan , Liu Caiyun , Zhu Min , Gao Hui , Cheng Ni , Cao Wei
Published: July 2019

Honey maturity is an important factor in evaluating the quality of honey. We established a method for the identification of natural mature acacia honey with eighteen physicochemical parameters combined with chemometric analysis. The analysis of variance showed significant differences between mature and immature acacia honey in physicochemical parameters. The principal component analysis explained 82.64% of the variance among samples, and indicated that total phenolic content, total protein content, and total sugar (glucose, fructose, sucrose) were the major variables. The cluster analysis and orthogonal partial least squares-discriminant analysis demonstrated that samples were grouped in relation to the maturity coinciding with the results of the principal component analysis. Meanwhile, the 35 test samples were classified with 100% accuracy with the method of multi-physicochemical parameters combined with chemometric analysis. All the results presented above proved the possibility of identifying mature acacia honey and immature acacia honey according to the chemometric analysis based on the multi-physicochemical parameters.
The Effect of Hybridized Carbon Nanotubes, Silica Nanoparticles, and Core-Shell Rubber on Tensile, Fracture Mechanics and Electrical Properties of Epoxy Nanocomposites
Authors: Bajpai Ankur , Carlotti Stéphane
Published: July 2019

The paper investigates the effect of adding a combination of rigid nanoparticles and core-shell rubber nanoparticles on the tensile, fracture mechanics, electrical and thermo-mechanical properties of epoxy resins. SiO2 nanoparticles, multi-walled carbon nanotubes (MWCNT’s), as rigid nanofillers, and core-shell rubber (CSR) nanoparticles, as soft nanofillers were used with bisphenol-A-based epoxy resin. Further, the rigid fillers were added systematically with core-shell rubber nanoparticles to investigate the commingled effect of rigid nanofillers and soft CSR nanoparticles. The resulting matrix will be broadly evaluated by standard methods to quantify tensile, fracture mechanics, electrical, and thermal properties. The results show that the electrical conductivity threshold is obtained at 0.075 wt. % for MWCNT-modified systems. For hybrid systems, the maximum increase of fracture toughness (218%) and fracture energy (900%) was obtained for a system containing 5 wt. % of CSR and 10 wt. % of SiO2. The analysis of the fracture surfaces revealed the information about existing toughening micro-mechanisms in the nanocomposites.
Agrp-Specific Ablation of Scly Protects against Diet-Induced Obesity and Leptin Resistance
Authors: Torres Daniel J. , Pitts Matthew W. , Hashimoto Ann C. , Berry Marla J.
Published: July 2019

Selenium, an essential trace element known mainly for its antioxidant properties, is critical for proper brain function and regulation of energy metabolism. Whole-body knockout of the selenium recycling enzyme, selenocysteine lyase (Scly), increases susceptibility to metabolic syndrome and diet-induced obesity in mice. Scly knockout mice also have decreased selenoprotein expression levels in the hypothalamus, a key regulator of energy homeostasis. This study investigated the role of selenium in whole-body metabolism regulation using a mouse model with hypothalamic knockout of Scly. Agouti-related peptide (Agrp) promoter-driven Scly knockout resulted in reduced weight gain and adiposity while on a high-fat diet (HFD). Scly-Agrp knockout mice had reduced Agrp expression in the hypothalamus, as measured by Western blot and immunohistochemistry (IHC). IHC also revealed that while control mice developed HFD-induced leptin resistance in the arcuate nucleus, Scly-Agrp knockout mice maintained leptin sensitivity. Brown adipose tissue from Scly-Agrp knockout mice had reduced lipid deposition and increased expression of the thermogenic marker uncoupled protein-1. This study sheds light on the important role of selenium utilization in energy homeostasis, provides new information on the interplay between the central nervous system and whole-body metabolism, and may help identify key targets of interest for therapeutic treatment of metabolic disorders.
Dietary Intake by Food Source and Eating Location in Low- and Middle-Income Chilean Preschool Children and Adolescents from Southeast Santiago
Authors: Rebolledo Natalia , Reyes Marcela , Corvalán Camila , Popkin Barry M. , Smith Taillie Lindsey
Published: July 2019

Background: Food source and eating location are important factors associated with the quality of dietary intake. In Chile the main food sources and eating locations of preschool children and adolescents and how these relate to dietary quality are unknown. Methods: We analyzed 24 h dietary recalls collected in 2016 from low- and middle-income Chilean preschool children (3–6 years, n = 839) and adolescents (12–14 years, n = 643) from southeastern Santiago. Surveys collected the food source and eating location for each food reported during the recall. We estimated the mean intake of calories and key nutrients of concern, such as saturated fats, total sugars, and sodium, by food source and eating location. Results: Foods obtained and eaten at home contributed the greatest proportion of total calories and the key nutrients of concern. Foods obtained at home tended to have lower caloric densities but higher sugar and sodium densities than foods obtained away from home in both age groups. With regard to location, for preschool children foods consumed at home had lower caloric and sugar densities than foods eaten at school, while for adolescents foods consumed at home had lower caloric, saturated fat, and sugar densities than foods eaten at school. For both children and adolescents, home was the primary source of sugar-sweetened beverages (SSBs) calories. SSBs were important calorie contributors among foods across all settings, but the highest absolute amount of calories from these beverages was consumed at home. Conclusions: While most of Chilean youths’ calories and key nutrients of concern are obtained and consumed at home, these foods tended to have lower caloric densities than foods obtained and consumed away from home. Home was the main food source for SSBs, but the relative consumption of these beverages was high in all eating locations. More research will be needed to inform and evaluate policies and interventions to improve children’s dietary quality across settings.
Feeding-Related Knowledge, Attitudes, and Practices among Grandparents in Singapore
Authors: Tan Bernadette Q.M. , Hee Jia Min , Yow Ka Shing , Sim Xueling , Asano Miho , Chong Mary Foong-Fong
Published: July 2019

Childhood obesity is a growing concern worldwide. Though multifactorial, the family environment exerts significant influence on children’s eating habits. Grandparents are increasingly involved as caregivers and they can significantly influence their grandchildren’s eating habits. Yet, literature on this topic is lacking. This exploratory sequential mixed methods study (qualitative interview and interviewer-administered questionnaire) aims to understand grandparents’ knowledge, attitudes, and practices on the feeding of their grandchildren in Singapore. A total of 11 interview participants and 396 questionnaire respondents with at least one grandchild, aged 12 years and below were included. Qualitative interviews informed the questionnaire development. Responses to interview questions about knowledge, attitudes, and practices revealed sub-themes such as knowledge on the impact of feeding, attitude toward feeding role, and challenges to feeding. Of the 396 participants, 35% were primary caregivers (defined as the person who spends the most time with the grandchild and performs most of the caregiving tasks). Nutritional knowledge was fair (median score 5/8), with misconceptions centered around healthy feeding practices. Grandparents who were primary caregivers, female, Malay, and younger than 70 years old believed that they played an important role in feeding their grandchild (p < 0.05). Overall, 47.2% of the grandparents rarely or never set a maximum limit on the amount of unhealthy food eaten, of which 77.1% are non-primary caregivers. In comparison, primary caregivers tend to set a maximum limit to the amount of unhealthy food their grandchildren eat and choose a wide variety of food (p < 0.05). These findings support the need for further improvement of grandparents’ feeding knowledge and practices as part of tackling childhood obesity.
Synthesis and Preclinical Evaluation of Radio-Iodinated GRPR/PSMA Bispecific Heterodimers for the Theranostics Application in Prostate Cancer
Authors: Abouzayed Ayman , Yim Cheng-Bin , Mitran Bogdan , Rinne Sara S. , Tolmachev Vladimir , Larhed Mats , Rosenström Ulrika , Orlova Anna
Published: July 2019

Gastrin-releasing peptide receptor (GRPR) and prostate-specific membrane antigen (PSMA) are overexpressed in most prostate cancers. GRPR expression is higher in early stages while PSMA expression increases with progression. The possibility of targeting both markers with a single theranostics radiotracer could improve patient management. Three GRPR/PSMA-targeting bispecific heterodimers (urea derivative PSMA-617 and bombesin-based antagonist RM26 linked via X-triazolyl-Tyr-PEG2, X = PEG2 (BO530), (CH2)8 (BO535), none (BO536)) were synthesized by solid-phase peptide synthesis. Peptides were radio-iodinated and evaluated in vitro for binding specificity, cellular retention, and affinity. In vivo specificity for all heterodimers was studied in PC-3 (GRPR-positive) and LNCaP (PSMA-positive) xenografts. [125I]I-BO530 was evaluated in PC-3pip (GRPR/PSMA-positive) xenografts. Micro single-photon emission computed tomography/computed tomography (microSPECT/CT) scans were acquired. The heterodimers were radiolabeled with high radiochemical yields, bound specifically to both targets, and demonstrated high degree of activity retention in PC-3pip cells. Only [125I]I-BO530 demonstrated in vivo specificity to both targets. A biodistribution study of [125I]I-BO530 in PC-3pip xenografted mice showed high tumor activity uptake (30%–35%ID/g at 3 h post injection (pi)). Activity uptake in tumors was stable and exceeded all other organs 24 h pi. Activity uptake decreased only two-fold 72 h pi. The GRPR/PSMA-targeting heterodimer [125I]I-BO530 is a promising agent for theranostics application in prostate cancer.
Controlled Shape and Porosity of Polymeric Colloids by Photo-Induced Phase Separation
Authors: Hadad Elad , Edri Eitan , Shpaisman Hagay
Published: July 2019

The shape and porosity of polymeric colloids are two properties that highly influence their ability to accomplish specific tasks. For micro-sized colloids, the control of both properties was demonstrated by the photo-induced phase separation of droplets of NOA81—a thiol-ene based UV-curable adhesive—mixed with acetone, water, and polyethylene glycol. The continuous phase was perfluoromethyldecalin, which does not promote phase separation prior to UV activation. A profound influence of the polymer concentration on the particle shape was observed. As the photo-induced phase separation is triggered by UV radiation, polymerization drives the extracted solution out of the polymeric matrix. The droplets of the extracted solution coalesce until they form a dimple correlated to the polymer concentration, significantly changing the shape of the formed solid colloids. Moreover, control could be gained over the porosity by varying the UV intensity, which governs the kinetics of the reaction, without changing the chemical composition; the number of nanopores was found to increase significantly at higher intensities.
Adsorption of Vi Capsular Antigen of Salmonella Typhi in Chitosan–Poly (Methacrylic Acid) Nanoparticles
Authors: da Silva Raimundo Lopes , da Silva Jaqueline Rodrigues , Júnior Anivaldo Pereira Duarte , Marinho Patrícia Santana Barbosa , Santos Lourivaldo Silva , Teixeira Francisco Martins , Júnior José Otávio Carréra Silva , Costa Roseane Maria Ribeiro
Published: July 2019

The development of a nanoparticulate system for the carrier antigen is now an important tool in the vaccination process, since a smaller number of doses is necessary for effective immunization. Thus, in this work a nanoparticulate system using polymers of chitosan and poly (methacrylic acid) (CS–PMAA) to adsorb the Vi antigen of Salmonella Typhi was developed. CS–PMAA nanoparticles with different proportions of chitosan and poly (methacrylic acid) were obtained and reached sizes from 123.9 ± 2.48 to 234.9 ± 2.66 nm, and spherical shapes were seen in transmission microscopy. At pH 7.2, the nanoparticles had a cationic surface charge that contributed to the adsorption of the Vi antigen. Qualitative analyses of the isolated Vi antigen were performed using Fourier-transform infrared spectroscopy, which indicated the presence of all the characteristic bands of the capsular polysaccharide, and nuclear magnetic resonance, which showed signals for the five hydrogens and the N-acetyl and O-acetyl groups which are characteristic of the Vi antigen structure. In the adsorption kinetics study, the Vi capsular antigen, contained in a phosphate buffer solution of pH 7.2, experienced 55% adsorption on the 1–1% (CS–PMAA) nanoparticles. The adsorption kinetics results showed the ability of the nanoparticulate system to adsorb the Vi antigen.
Spatially Engraving Morphological Structure on a Polymeric Surface by Ion Beam Milling
Authors: Sun Ansu , Wang Ding , Zhou Honghao , Li Yifan , Connor Chris , Kong Jie , Sun Jining , Xu Ben Bin
Published: July 2019

Polymer surface patterning and modification at the micro/nano scale has been discovered with great impact in applications such as microfluidics and biomedical technologies. We propose a highly efficient fabricating strategy, to achieve a functional polymer surface, which has control over the surface roughness. The key development in this fabrication method is the polymer positive diffusion effect (PDE) for an ion-bombarded polymeric hybrid surface through focused ion beam (FIB) technology. The PDE is theoretically explored by introducing a positive diffusion term into the classic theory. The conductivity-induced PDE constant is discussed as functions of substrates conductivity, ion energy and flux. The theoretical results agree well with the experiential results on the conductivity-induced PDE, and thus yield good control over roughness and patterning milling depth on the fabricated surface. Moreover, we demonstrate a controllable surface wettability in hydrophobic and superhydrophobic surfaces (contact angles (CA) range from 108.3° to 150.8°) with different CA hysteresis values ranging from 31.4° to 8.3°.
Purification of Polybutylene Terephthalate by Oligomer Removal Using a Compressed CO2 Antisolvent
Authors: Yu Wen , Huang Jian-Hao , Tan Chung-Sung
Published: July 2019

In this study, the cyclic oligomers in the highly chemically resistant polyester polybutylene terephthalate (PBT) were effectively removed using a compressed CO2 antisolvent technique in which 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) was used as the solvent. In addition to the oligomers, tetrahydrofuran was completely removed because of its low molecular weight and liquid state. The effects of the operating variables, including temperature, pressure, and the PBT concentration in HFIP, on the degree of removal of the oligomers were systematically studied using experimental design and the response surface methodology. The most appropriate operating conditions for the purification of PBT were 8.3 MPa and 23.4 °C when using 4.5 wt % PBT in HFIP. Under these conditions, the cyclic trimers and dimers could be removed by up to 81.4% and 95.7%, respectively, in a very short operating time.
Impacts of Land-Use Changes on Soil Erosion in Water–Wind Crisscross Erosion Region of China
Authors: Wang Jie , Zhang Weiwei , Zhang Zengxiang
Published: July 2019

Soil erosion affects food production, biodiversity, biogeochemical cycles, hydrology, and climate. Land-use changes accelerated by intensive human activities are a dominant anthropogenic factor inducing soil erosion globally. However, the impacts of land-use-type changes on soil erosion dynamics over a continuous period for constructing a sustainable ecological environment has not been systematically quantified. This study investigates the spatial–temporal dynamics of land-use change and soil erosion across a specific area in China with water–wind crisscross erosion during three periods: 1995–1999, 2000–2005, and 2005–2010. We analyzed the impacts of each land-use-type conversion on the intensity changes of soil erosion caused by water and wind, respectively. The major findings include: (1) land-use change in the water–wind crisscross erosion region of China was characterized as cultivated land expansion at the main cost of grassland during 1995–2010; (2) the strongest land-use change moved westward in space from the central Loess Plateau area in 1995–2005 to the western piedmont alluvial area in 2005–2010; (3) soil erosion area is continuously increasing, but the trend is declining from the late 1990s to the late 2000s; (4) the soil conservation capability of land-use types in water–wind crisscross erosion regions could be compiled from high to low as high coverage grasslands, medium coverage grasslands, paddy, drylands, low coverage grasslands, built-up lands, unused land of sandy lands, the Gobi Desert, and bare soil. These findings could provide some insights for executing reasonable land-use approaches to balance human demands and environment sustainability.
RTK with the Assistance of an IMU-Based Pedestrian Navigation Algorithm for Smartphones
Authors: Niu Zun , Nie Ping , Tao Lin , Sun Junren , Zhu Bocheng
Published: July 2019

Real-time kinematic (RTK) technique is widely used in modern society because of its high accuracy and real-time positioning. The appearance of Android P and the application of BCM47755 chipset make it possible to use single-frequency RTK and dual-frequency RTK on smartphones. The Xiaomi Mi 8 is the first dual-frequency Global Navigation Satellite System (GNSS) smartphone equipped with BCM47755 chipset. However, the performance of RTK in urban areas is much poorer compared with its performance under the open sky because the satellite signals can be blocked by the buildings and trees. RTK can't provide the positioning results in some specific areas such as the urban canyons and the crossings under an overpass. This paper combines RTK with an IMU-based pedestrian navigation algorithm. We utilize attitude and heading reference system (AHRS) algorithm and zero velocity update (ZUPT) algorithm based on micro electro mechanical systems (MEMS) inertial measurement unit (IMU) in smartphones to assist RTK for the sake of improving positioning performance in urban areas. Some tests are carried out to verify the performance of RTK on the Xiaomi Mi 8 and we respectively assess the performances of RTK with and without the assistance of an IMU-based pedestrian navigation algorithm in urban areas. Results on actual tests show RTK with the assistance of an IMU-based pedestrian navigation algorithm is more robust and adaptable to complex environments than that without it.