bluuebunny/crossref_metadata_2025 huggingface dataset:
Lewy pathology of the esophagus correlates with the progression of Lewy body disease: a Japanese cohort study of autopsy cases
Authors: Tanei Zen-ichi , Saito Yuko , Ito Shinji , Matsubara Tomoyasu , Motoda Atsuko , Yamazaki Mikihiro , Sakashita Yasuhiro , Kawakami Ito , Ikemura Masako , Tanaka Shinya , Sengoku Renpei , Arai Tomio , Murayama Shigeo
Published: November 2020

Lewy body disease (LBD) is a spectrum of progressive neurodegenerative disorders characterized by the wide distribution of Lewy bodies and neurites in the central and peripheral nervous system (CNS, PNS). Clinical diagnoses include Parkinson’s disease (PD), dementia with Lewy bodies, or pure autonomic failure. All types of LBD are accompanied by non-motor symptoms (NMSs) including gastrointestinal dysfunctions such as constipation. Its relationship to Lewy body-related α-synucleinopathy (Lewy pathology) of the enteric nervous system (ENS) is attracting attention because it can precede the motor symptoms. To clarify the role of ENS Lewy pathology in disease progression, we performed a clinicopathological study using the Brain Bank for Aging Research in Japan. Five-hundred and eighteen cases were enrolled in the study. Lewy pathology of the CNS and PNS, including the lower esophagus as a representative of the ENS, was examined via autopsy findings. Results showed that one-third of older people (178 cases, 34%) exhibited Lewy pathology, of which 78 cases (43.8%) exhibited the pathology in the esophagus. In the esophageal wall, Auerbach’s plexus (41.6%) was most susceptible to the pathology, followed by the adventitia (33.1%) and Meissner’s plexus (14.6%). Lewy pathology of the esophagus was significantly associated with autonomic failures such as constipation (p < 0.0001) and among PNS regions, correlated the most with LBD progression (r = 0.95, p < 0.05). These findings suggest that the propagation of esophageal Lewy pathology is a predictive factor of LBD.
Anger and aggressiveness in obsessive–compulsive disorder (OCD) and the mediating role of responsibility, non-acceptance of emotions, and social desirability
Authors: Cludius Barbara , Mannsfeld Anna K. , Schmidt Alexander F. , Jelinek Lena
Published: November 2020

According to psychodynamic and cognitive models of obsessive–compulsive disorder (OCD), anger and aggression play an important role in the development and maintenance of the disorder. (Sub-) clinical samples with OCD have reported higher anger and anger suppression. Patients with checking-related symptoms of OCD showed a less aggressive self-concept as assessed by an Implicit Association Test (IAT). This study assessed anger and aggressiveness self-concepts in OCD as well as possible mediators of the link between OCD and aggressiveness. A total of 48 patients with OCD and 45 healthy controls were included. Measures included the State-Trait Anger Expression Inventory-II and an aggressiveness self-concept IAT (Agg-IAT). An inflated sense of responsibility, non-acceptance of emotions, and social desirability were tested as mediators. As expected, patients with OCD reported higher trait anger and anger suppression compared to healthy controls. Contrary to hypotheses, the aggressiveness self-concept (Agg-IAT) did not differ between groups. The inflated sense of responsibility mediated the relationship between group and anger suppression. Non-acceptance of negative emotions mediated the relationship between group and trait anger, as well as anger suppression. However, comorbidities and medication may account for some effect in anger suppression. Elevated trait anger and anger suppression in OCD patients could be explained by dysfunctional beliefs or maladaptive emotion regulation strategies. Emotion regulation therapy might help to enhance awareness and acceptance of emotions and possibly improve treatment outcomes.
Baroreflex responses during dry resting and exercise apnoeas in air and pure oxygen
Authors: Taboni Anna , Vinetti Giovanni , Fontolliet Timothée , Grasso Gabriele Simone , Tam Enrico , Moia Christian , Ferretti Guido , Fagoni Nazzareno
Published: November 2020

Purpose

We analysed the characteristics of arterial baroreflexes during the first phase of apnoea (φ1).

Methods

12 divers performed rest and exercise (30 W) apnoeas (air and oxygen). We measured beat-by-beat R-to-R interval (RRi) and mean arterial pressure (MAP). Mean RRi and MAP values defined the operating point (OP) before (PRE-ss) and in the second phase (φ2) of apnoea. Baroreflex sensitivity (BRS, ms·mmHg−1) was calculated with the sequence method.

Results

In PRE-ss, BRS was (median [IQR]): at rest, 20.3 [10.0–28.6] in air and 18.8 [13.8–25.2] in O2; at exercise 9.2[8.4–13.2] in air and 10.1[8.4–13.6] in O2. In φ1, during MAP decrease, BRS was lower than in PRE-ss at rest (6.6 [5.3–11.4] in air and 7.7 [4.9–14.3] in O2, p < 0.05). At exercise, BRS in φ1 was 6.4 [3.9–13.1] in air and 6.7 [4.1–9.5] in O2. After attainment of minimum MAP (MAPmin), baroreflex resetting started. After attainment of minimum RRi, baroreflex sequences reappeared. In φ2, BRS at rest was 12.1 [9.6–16.2] in air, 12.9 [9.2–15.8] in O2. At exercise (no φ2 in air), it was 7.9 [5.4–10.7] in O2. In φ2, OP acts at higher MAP values.

Conclusion

In apnoea φ1, there is a sudden correction of MAP fall via baroreflex. The lower BRS in the earliest φ1 suggests a possible parasympathetic mechanism underpinning this reduction. After MAPmin, baroreflex resets, displacing its OP at higher MAP level; thus, resetting may not be due to central command. After resetting, restoration of BRS suggests re-establishment of vagal drive.
Dynamics Analysis of Active Variable Stiffness Vibration Isolator for Whole-Spacecraft Systems Based on Nonlinear Output Frequency Response Functions
Authors: Xu Kefan , Zhang Yewei , Zhu Yunpeng , Zang Jian , Chen Liqun
Published: November 2020

In order to improve the harsh dynamic environment experienced by heavy rockets during different external excitations, this study presents a novel active variable stiffness vibration isolator (AVS-VI) used as the vibration isolation device to reduce excessive vibration of the whole-spacecraft isolation system. The AVS-VI is composed of horizontal stiffness spring, positive stiffness spring, parallelogram linkage mechanism, piezoelectric actuator, acceleration sensor, viscoelastic damping, and PID active controller. Based on the AVS-VI, the generalized vibration transmissibility determined by the nonlinear output frequency response functions and the energy absorption rate is applied to analyze the isolation performance of the whole-spacecraft system with AVS-VI. The AVS-VI can conduct adaptive vibration suppression with variable stiffness to the whole-spacecraft system, and the analysis results indicate that the AVS-VI is effective in reducing the extravagant vibration of the whole-spacecraft system, where the vibration isolation is decreased up to above 65% under different acceleration excitations. Finally, different parameters of AVS-VI are considered to optimize the whole-spacecraft system based on the generalized vibration transmissibility and the energy absorption rate.
Improving the lubrication and anti-corrosion performance of polyurea grease via ingredient optimization
Authors: Ren Guanlin , Sun Xiaowen , Li Wen , Li Hao , Zhang Lin , Fan Xiaoqiang , Li Dongshan , Zhu Minhao
Published: November 2020

Thickener formulation plays a significant role in the performance characteristics of grease. The polyurea greases (PUGs) were synthesized using mineral oil (500SN) as the base oil, and by regulating the reaction of diphenylmethane diisocyanate (MDI) and different organic amines. The as-prepared PUGs from the reaction of MDI and cyclohexylamine/p-toluidine exhibit the optimum physicochemical and friction-wear properties, confirming that the regulation of thickener formulation can improve the performance characteristics of grease, including friction reduction, wear, corrosion resistance, and load-carrying capacity. The anti-corrosion and lubrication properties of as-prepared PUGs depend on good sealing functions and a boundary lubrication film (synergy of grease-film and tribo-chemical reaction film), as well as their chemical components and structure.
Tribo-informatics: Concept, architecture, and case study
Authors: Zhang Zhinan , Yin Nian , Chen Shi , Liu Chengliang
Published: November 2020

Friction plays a vital role in energy dissipation, device failure, and even energy supply in modern society. After years of research, data and information on tribology research are becoming increasingly available. Because of the strong systematic and multi-disciplinary coupling characteristics of tribology, tribology information is scattered in various disciplines with different patterns, e.g., technical documents, databases, and papers, thereby increasing the information entropy of the system, which is inconducive to the preservation and circulation of research information. With the development of computer and information science and technology, many subjects have begun to be combined with information technology, and multi-disciplinary informatics has been born. This paper describes the combination of information technology with tribology research, presenting the connotation and architecture of tribo-informatics, and providing a case study on implementing the proposed concept and architecture. The proposal and development of tribo-informatics described herein will improve the research efficiency and optimize the research process of tribology, which is of considerable significance to the development of this field.
Émilie Du Châtelet on Illusions
Authors: LASCANO MARCY P.
Published: November 2020

In her Discourse on Happiness, Émilie du Châtelet argues susceptibility to illusion is one of the five ‘great machines of happiness,’ and that ‘we owe most of our pleasures to illusions’ (2009: 349). However, many who read the Discourse find this aspect of her view puzzling and in tension with her claims that we must always seek truth and obey reason. To understand better her claims in the Discourse on Happiness, this article explores Du Châtelet's discussions of illusions in her Foundations of Physics, On Liberty, and the Dissertation on the Nature and Propagation of Fire. I distinguish four types of illusions that Du Châtelet posits and clarify the ways in which these relate to her views on happiness and love in the Discourse and argue that she avoids deceptive or perpetual illusions of happiness through the use of the principle of sufficient reason.
Creating effective academic research teams: Two tools borrowed from business practice
Authors: Brower Holly H. , Nicklas Barbara J. , Nader Michael A. , Trost Lindsay M. , Miller David P.
Published: November 2020

Academic Medical Centers strive to create multidisciplinary research teams to produce impactful science. However, few faculty researchers receive training in “team science,” a well-established concept in business research and practice. Responding to demand for assistance developing effective research teams, the Collaboration and Team Science Program of the Clinical and Translational Science Institute (CTSI) at Wake Forest School of Medicine (WFSM) partnered with faculty from the Wake Forest University (WFU) School of Business with expertise in leadership, management, and team building. We initiated a needs assessment, including a written survey from a diverse set of 42 research scientists as well as semi-structured interviews with 8 researchers. In response to identified needs, we developed training sessions and consultations to teach teams to implement two tools known to enhance team dynamics: (1) Team charter, a document that defines the team’s purpose, goals, roles, and strategies; and (2) Responsible, Accountable, Consulted, Informed (RACI) matrix, a table or spreadsheet that clarifies tasks and accountability. Since 2018, 10 teams and over 100 individuals have attended training sessions and 6 teams received personalized team consults. We describe these tools, present a formal analysis of quantitative results, and highlight the next steps being taken in response to these findings.
Hospital Visitors’ Awareness and Adaptation of Preventive Measures for Middle East Respiratory Syndrome Coronavirus
Authors: Aljarallah Khalid M. , Alrukban Mohammed O. , Alghamdi Yazeed S. , Alanazi Bandar S. , Alturki Khaled E. , Alkhunayfir Hamad A. , Alshammari Madloul J. , Alamri Fahad M. , Hamadi Hanadi Y.
Published: November 2020

Objective:

This study aims to assess the knowledge and awareness, and to identify the practice reflection of knowledge concerning Middle East Respiratory Syndrome (MERS) on hospital visitor’s daily life.

Methods:

A cross-sectional study, conducted in 2 tertiary referral hospitals in Riyadh Saudi Arabia, from February 2015 to February 2016. A total random sample of 305 hospital visitors consented to participate. Data were collected through a self-administered questionnaire consisting of questions regarding awareness and practice of measures to prevent the spread of infection.

Results:

Study showed that participants have a fair knowledge regarding the cause of MERS (N = 228; 74.8%). Nearly half of them (47%) stated that camels are the source of the spread of MERS. Approximately 70% of the participants preferred both sanitization and wearing facemasks as preventive measures for MERS. However, only 3.95% practiced not eating camel products, such as milk and meat.

Conclusions:

Although hospital visitors showed some knowledge and positive awareness in several aspects of MERS awareness, there are weak areas where knowledge and awareness were not up to recommended guidelines. Continued educational programs are needed to improve awareness and knowledge of all the public toward MERS-coronavirus infection. This study may assist in the development of future strategies on preventive measures of the disease.
Divergent trajectories in arithmetic homogeneous spaces of rational rank two
Authors: TAMAM NATTALIE
Published: November 2020

Let G be a semisimple real algebraic group defined over

${\mathbb {Q}}$
,

$\Gamma $
be an arithmetic subgroup of G, and T be a maximal

${\mathbb {R}}$
-split torus. A trajectory in

$G/\Gamma $
is divergent if eventually it leaves every compact subset. In some cases there is a finite collection of explicit algebraic data which accounts for the divergence. If this is the case, the divergent trajectory is called obvious. Given a closed cone in T, we study the existence of non-obvious divergent trajectories under its action in

$G\kern-1pt{/}\kern-1pt\Gamma $
. We get a sufficient condition for the existence of a non-obvious divergence trajectory in the general case, and a full classification under the assumption that

$\mathrm {rank}_{{\mathbb {Q}}}G=\mathrm {rank}_{{\mathbb {R}}}G=2$
.
Articulated trilobite ontogeny: suggestions for a methodological standard
Authors: Hughes Nigel C. , Adrain Jonathan M. , Holmes James D. , Hong Paul S. , Hopkins Melanie J. , Hou Jin-Bo , Minelli Alessandro , Park Tae-Yoon S. , Paterson John R. , Peng Jin , Webster Mark , Zhang Xi-Guang , Zhang Xing-Liang , Fusco Giuseppe
Published: November 2020

In order to maximize the utility of future studies of trilobite ontogeny, we propose a set of standard practices that relate to the collection, nomenclature, description, depiction, and interpretation of ontogenetic series inferred from articulated specimens belonging to individual species. In some cases, these suggestions may also apply to ontogenetic studies of other fossilized taxa.
The role of haematological parameters in patients with COVID-19 and influenza virus infection
Authors: Kazancioglu Sumeyye , Bastug Aliye , Ozbay Bahadir Orkun , Kemirtlek Nizamettin , Bodur Hurrem
Published: November 2020

SARS-CoV-2, the causative agent of coronavirus disease 19 (COVID-19), was identified in Wuhan, China. Since then, the novel coronavirus started to be compared to influenza. The haematological parameters and inflammatory indexes are associated with severe illness in COVID-19 patients. In this study, the laboratory data of 120 COVID-19 patients, 100 influenza patients and 61 healthy controls were evaluated. Lower lymphocytes, eosinophils, basophils, platelets and higher delta neutrophil index (DNI), neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) were found in COVID-19 and influenza groups compared to healthy controls. The eosinophils, lymphocytes and PLR made the highest contribution to differentiate COVID-19 patients from healthy controls (area under the curves (AUCs): 0.819, 0.817 and 0.716, respectively; P-value is <0.0001 for all). The NLR, the optimal cut-off value was 3.58, which resulted in a sensitivity of 30.8 and a specificity of 100 (AUC: 0.677, P < 0.0001). Higher leucocytes, neutrophils, DNI, NLR, PLR and lower lymphocytes, red blood cells, haemoglobin, haematocrit levels were found in severe patients at the end of treatment. Nonsevere patients showed an upward trend for lymphocytes, eosinophils and platelets, and a downward trend for neutrophils, DNI, NLR and PLR. However, there was an increasing trend for eosinophils, platelets and PLR in severe patients. In conclusion, NLR and PLR can be used as biomarkers to distinguish COVID-19 patients from healthy people and to predict the severity of COVID-19. The increasing value of PLR during follow-up may be more useful compared to NLR to predict the disease severity.
Does language switching behavior rely on general executive functions?
Authors: Jylkkä Jussi , Laine Matti , Lehtonen Minna
Published: November 2020

The assumption that everyday language switching trains bilinguals’ executive functions (EF) presupposes that language switching engages domain-general EF. This study examined associations between three types of tasks in a group of Finnish-English late bilinguals: everyday language switching frequency assessed with Ecological Momentary Assessment, language switching performance on a cued bilingual naming task, and EF as measured with Simon, Flanker, and Number-letter tasks. Cued switching performance showed mainly positive associations with EF performance, but the associations between everyday language switching and cued switching performance, and between everyday switching and the EF tasks were largely against our hypotheses. The findings indicate that participants with lower monitoring capacity make more everyday language switches. This speaks against the idea that everyday language switching would facilitate executive functioning. The results suggest that associations between language switching and general EF are more complex than current models assume.
Savarkar before Hindutva: Sovereignty, Republicanism, and Populism in India,c.1900–1920
Authors: Visana Vikram
Published: November 2020

Vinayak Damodar Savarkar was the theorizer of Hindutva (1923)—the project to radically reconfigure India as a Hindu majoritarian state. Assessments of Savarkar's earlierThe Indian War of Independence(1909), a history of the 1857 Indian “Mutiny,” have generally subsumed this tract into the logic of Hindutva. This article offers a reassessment ofThe Indian War of Independenceand situates it within the political and intellectual context offin de sièclewestern India. I suggest that this history of Indian rebellion propagated a novel iteration of Indian popular sovereignty predicated on Hindu–Muslim unity. I read Savarkar as adapting the ideas of Giuseppe Mazzini and Johann Kaspar Bluntschli to challenge what he regarded as the fissiparous logic of late colonial liberalism. Finally, this article argues that Savarkar's account of the mutual constitution of general will and the personalism of sovereignty must be read as a previously unacknowledged instance of Indian populism.
Incentivizing physical activity through activity monitoring interventions in PAD – a pilot study
Authors: Fukaya Eri , Welden Scott , Bukari Abdallah , Khan Zeeshan , Leeper Nicholas , Mohler Emile
Published: November 2020

Summary: Background: There is ample evidence to show that supervised exercise is efficacious and cost effective for improving claudication symptoms in patients with peripheral artery disease (PAD). Home based exercise therapy can be an effective alternative to supervised exercise however, the results of this is variable depending on the level of motivation and engagement of the patient. Patients and methods: We performed a pilot study in 41 patients to determine whether a home based exercise program with the use of an activity tracking device with personalized feedback and financial incentives can increase daily activity, improve walking and sustain engagement in the exercise regimen in patients with PAD. In this randomized pilot study, the patients in the study group were fitted with an activity monitoring device and given behavioral monitoring, motivational updates and feedback regarding their exercise program. This study group was further divided in to two groups. One half of these patients in the study group were also given financial incentives if they reached their set targets. The control group wore the device with no feedback or ability to see their number of steps walked. Results: Results showed that at the end of the 12 week period, patients in the study groups walked more compared to the controls and the financial incentive structure resulted in an additional 38–63% increase in average daily steps. Conclusions: This pilot study revealed that a home-based exercise program with activity monitoring, feedback and financial incentives resulted increased daily steps, 6-minute walking distance and overall compliance with the program in PAD patients with claudication.
A Major Combustion Aerosol Event Had a Negligible Impact on the Atmospheric Ice‐Nucleating Particle Population
Authors: Adams M. P. , Tarn M. D. , Sanchez‐Marroquin A. , Porter G. C. E. , O'Sullivan D. , Harrison A. D. , Cui Z. , Vergara‐Temprado J. , Carotenuto F. , Holden M. A. , Daily M. I. , Whale T. F. , Sikora S. N. F. , Burke I. T. , Shim J.‐U. , McQuaid J. B. , Murray B. J.
Published: November 2020

Clouds containing supercooled water are important for both climate and weather, but our knowledge of which aerosol particle types nucleate ice in these clouds is far from complete. Combustion aerosols have strong anthropogenic sources, and if these aerosol types were to nucleate ice in clouds, they might exert a climate forcing. Here, we quantified the atmospheric ice‐nucleating particle (INP) concentrations during the United Kingdom's annual Bonfire Night celebrations, which are characterized by large amounts of combustion aerosol from bonfires and fireworks. We used three immersion mode techniques covering more than 6 orders of magnitude in INP concentration over the temperature range from −10°C to homogeneous freezing. We found no observable systematic change in the INP concentration on three separate nights, despite more than a factor of 10 increase in aerosol number concentrations, up to a factor of 10 increase in PM10 concentration, and more than a factor of 100 increase in black carbon (BC) mass concentration relative to pre‐event levels. This implies that BC and other combustion aerosol such as ash did not compete with the INPs present in the background air. Furthermore, the upper limit of the ice‐active site surface density, ns(T), of BC generated in these events was shown to be consistent with several other recent laboratory studies, showing a very low ice‐nucleating activity of BC. We conclude that combustion aerosol particles similar to those emitted on Bonfire Night are at most of secondary importance for the INP population relevant for mixed‐phase clouds in typical midlatitude terrestrial locations.
Parameterizing the Impact of Unresolved Temperature Variability on the Large‐Scale Density Field: Part 1. Theory.
Authors: Stanley Z. , Grooms I. , Kleiber W. , Bachman S. D. , Castruccio F. , Adcroft A.
Published: November 2020

Unresolved temperature and salinity fluctuations interact with a nonlinear seawater equation of state to produce significant errors in the ocean model evaluation of the large‐scale density field. It is shown that the impact of temperature fluctuations dominates the impact of salinity fluctuations and that the error in density is, to leading order, proportional to the product of a subgrid‐scale temperature variance and a second derivative of the equation of state. Two parameterizations are proposed to correct the large‐scale density field: one deterministic and one stochastic. Free parameters in both parameterizations are fit using fine‐resolution model data. Both parameterizations are computationally efficient as they require only one additional evaluation of a nonlinear equation at each grid cell. A companion paper will discuss the climate impacts of the parameterizations proposed here.
Beyond Imperviousness: The Role of Antecedent Wetness in Runoff Generation in Urbanized Catchments
Authors: Saadi Mohamed , Oudin Ludovic , Ribstein Pierre
Published: November 2020

Explaining the response of catchments with a mix of pervious and impervious surfaces requires the inclusion of controls other than catchment imperviousness. In this study, we made use of a large sample of 70,227 events in 852 U.S. catchments to analyze the interplay of total impervious area (TIA) and antecedent soil moisture (ASM) in determining the event runoff ratio (RR). First, we investigated the importance of TIA as a spatial control compared to other catchment attributes (describing climate and catchment landscape) in explaining the differences between catchments in terms of median event‐scale RR. Second, we examined the importance of ASM as a temporal control of the variability of RR in comparison with spatial settings and event characteristics. Third, events were grouped into 30 classes of TIA and 30 classes of ASM, and linear regression models were used to analyze the evolution of RR depending on each one of the two independent variables. Results showed the following: (1) Analysis of the importance of space‐varying catchment descriptors highlighted the relevance of TIA as a control of catchment response, whereas ASM showed a stronger ability compared to other event characteristics. (2) ASM played a significant role in urbanized catchments as in rural ones. The absence of abrupt changes in the linear model parameters indicates that the impact of urbanization on the relationship between RR and ASM was relatively gradual. (3) Differences in RR between rural and intensively urbanized catchments were observable not only for dry pre‐event conditions but also for wet short‐term conditions.
Macrophages produce and functionally respond to interleukin-34 in colon cancer
Authors: Franzè Eleonora , Laudisi Federica , Di Grazia Antonio , Marônek Martin , Bellato Vittoria , Sica Giuseppe , Monteleone Giovanni
Published: November 2020

In colorectal cancer (CRC), macrophages represent a major component of the tumor mass and exert mostly functions promoting tumor cell survival, proliferation, and dissemination. Interleukin-34 (IL-34) is a cytokine overproduced by colon cancer (CRC) cells and supposed to make a valid contribution to the growth and diffusion of CRC cells. The biological functions of IL-34 are mediated by the macrophage colony-stimulating factor receptor (M-CSFR-1), which controls monocyte/macrophage differentiation, growth, and survival. We here investigated whether, in CRC, tumor-associated macrophages (TAMs) express M-CSFR-1 and functionally respond to IL-34. By flow-cytometry analysis of tumor-infiltrating cells (TICs) and lamina propria mononuclear cells (LPMCs) isolated from normal, adjacent mucosa of CRC patients, we showed that CD68/HLA-DRII-expressing TICs and LPMCs expressed M-CSFR-1. Both these cell types produced IL-34 even though the expression of the cytokine was more pronounced in TICs as compared to normal LPMCs. Moreover, in CRC samples, there was a positive correlation between IL-34-producing cells and CD68-positive cells. Stimulation of LPMCs and TICs with IL-34 resulted in enhanced expression of CD163 and CD206, two markers of type II-polarized macrophages, and this was evident at both RNA and protein level. In the same cell cultures, IL-34 stimulated expression and production of IL-6, a cytokine known to promote CRC cell growth and diffusion. Finally, knockdown of IL-34 in TICs with specific antisense oligonucleotides with: a specific antisesne oligonucleotide decreased IL-6 production and the number of TAMs producing this cytokine. This is the first to show a positive role of IL-34 in the control of TAMs in CRC, further supporting the notion that IL-34 sustains colon tumorigenesis.
Fractons from frustration in hole-doped antiferromagnets
Authors: Sous John , Pretko Michael
Published: November 2020

Recent theoretical research on tensor gauge theories led to the discovery of an exotic type of quasiparticles, dubbed fractons, that obey both charge and dipole conservation. Here we describe physical implementation of dipole conservation laws in realistic systems. We show that fractons find a natural realization in hole-doped antiferromagnets. There, individual holes are largely immobile, while dipolar hole pairs move with ease. First, we demonstrate a broad parametric regime of fracton behavior in hole-doped two-dimensional Ising antiferromagnets viable through five orders in perturbation theory. We then specialize to the case of holes confined to one dimension in an otherwise two-dimensional antiferromagnetic background, which can be realized via the application of external fields in experiments, and prove ideal fracton behavior. We explicitly map the model onto a fracton Hamiltonian featuring conservation of dipole moment. Manifestations of fractonicity in these systems include gravitational clustering of holes. We also discuss diagnostics of fracton behavior, which we argue is borne out in existing experimental results.
Chi hotspot control of RecBCD helicase-nuclease by long-range intramolecular signaling
Authors: Amundsen Susan K. , Taylor Andrew F. , Smith Gerald R.
Published: November 2020

Repair of broken DNA by homologous recombination requires coordinated enzymatic reactions to prepare it for interaction with intact DNA. The multiple activities of enterobacterial RecBCD helicase-nuclease are coordinated by Chi recombination hotspots (5′ GCTGGTGG 3′) recognized during DNA unwinding. Chi is recognized in a tunnel in RecC but activates the RecB nuclease, > 25 Ǻ away. How the Chi-dependent signal travels this long distance has been unknown. We found a Chi hotspot-deficient mutant in the RecB helicase domain located > 45 Ǻ from both the Chi-recognition site and the nuclease active site. This unexpected observation led us to find additional mutations that reduced or eliminated Chi hotspot activity in each subunit and widely scattered throughout RecBCD. Each mutation alters the intimate contact between one or another pair of subunits in crystal or cryoEM structures of RecBCD bound to DNA. Collectively, these mutations span a path about 185 Ǻ long from the Chi recognition site to the nuclease active site. We discuss these surprising results in the context of an intramolecular signal transduction accounting for many previous observations.
Amalgamation of 3D structure and sequence information for protein–protein interaction prediction
Authors: Jha Kanchan , Saha Sriparna
Published: November 2020

Protein is the primary building block of living organisms. It interacts with other proteins and is then involved in various biological processes. Protein–protein interactions (PPIs) help in predicting and hence help in understanding the functionality of the proteins, causes and growth of diseases, and designing new drugs. However, there is a vast gap between the available protein sequences and the identification of protein–protein interactions. To bridge this gap, researchers proposed several computational methods to reveal the interactions between proteins. These methods merely depend on sequence-based information of proteins. With the advancement of technology, different types of information related to proteins are available such as 3D structure information. Nowadays, deep learning techniques are adopted successfully in various domains, including bioinformatics. So, current work focuses on the utilization of different modalities, such as 3D structures and sequence-based information of proteins, and deep learning algorithms to predict PPIs. The proposed approach is divided into several phases. We first get several illustrations of proteins using their 3D coordinates information, and three attributes, such as hydropathy index, isoelectric point, and charge of amino acids. Amino acids are the building blocks of proteins. A pre-trained ResNet50 model, a subclass of a convolutional neural network, is utilized to extract features from these representations of proteins. Autocovariance and conjoint triad are two widely used sequence-based methods to encode proteins, which are used here as another modality of protein sequences. A stacked autoencoder is utilized to get the compact form of sequence-based information. Finally, the features obtained from different modalities are concatenated in pairs and fed into the classifier to predict labels for protein pairs. We have experimented on the human PPIs dataset and Saccharomyces cerevisiae PPIs dataset and compared our results with the state-of-the-art deep-learning-based classifiers. The results achieved by the proposed method are superior to those obtained by the existing methods. Extensive experimentations on different datasets indicate that our approach to learning and combining features from two different modalities is useful in PPI prediction.
Author Correction: Continuous scanning for Bragg coherent X-ray imaging
Authors: Li Ni , Dupraz Maxime , Wu Longfei , Leake Steven J. , Resta Andrea , Carnis Jérôme , Labat Stéphane , Almog Ehud , Rabkin Eugen , Favre-Nicolin Vincent , Picca Frédéric-Emmanuel , Berenguer Felisa , van de Poll Rim , Hofmann Jan P. , Vlad Alina , Thomas Olivier , Garreau Yves , Coati Alessandro , Richard Marie-Ingrid
Published: November 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Deposits from giant floods in Gale crater and their implications for the climate of early Mars
Authors: Heydari E. , Schroeder J. F. , Calef F. J. , Van Beek J. , Rowland S. K. , Parker T. J. , Fairén A. G.
Published: November 2020

This study reports in-situ sedimentologic evidence of giant floods in Gale crater, Mars, during the Noachian Period. Features indicative of floods are a series of symmetrical, 10 m-high gravel ridges that occur in the Hummocky Plains Unit (HPU). Their regular spacing, internal sedimentary structures, and bedload transport of fragments as large as 20 cm suggest that these ridges are antidunes: a type of sedimentary structure that forms under very strong flows. Their 150 m wavelength indicates that the north-flowing water that deposited them was at least 24 m deep and had a minimum velocity of 10 m/s. Floods waned rapidly, eroding antidune crests, and re-deposited removed sediments as patches on the up-flow limbs and trough areas between these ridges forming the Striated Unit (SU). Each patch of the SU is 50–200 m wide and long and consists of 5–10 m of south-dipping layers. The strike and dip of the SU layers mimic the attitude of the flank of the antidune on which they were deposited. The most likely mechanism that generated flood waters of this magnitude on a planet whose present-day average temperature is − 60 °C was the sudden heat produced by a large impact. The event vaporized frozen reservoirs of water and injected large amounts of CO2 and CH4 from their solid phases into the atmosphere. It temporarily interrupted a cold and dry climate and generated a warm and wet period. Torrential rainfall occurred planetwide some of which entered Gale crater and combined with water roaring down from Mt. Sharp to cause gigantic flash floods that deposited the SU and the HPU on Aeolis Palus. The warm and wet climate persisted even after the flooding ended, but its duration cannot be determined by our study.
Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response
Authors: Bensoussan Nicolas , Dixit Sameer , Tabara Midori , Letwin David , Milojevic Maja , Antonacci Michele , Jin Pengyu , Arai Yuka , Bruinsma Kristie , Suzuki Takeshi , Fukuhara Toshiyuki , Zhurov Vladimir , Geibel Sven , Nauen Ralf , Grbic Miodrag , Grbic Vojislava
Published: November 2020

Comprehensive understanding of pleiotropic roles of RNAi machinery highlighted the conserved chromosomal functions of RNA interference. The consequences of the evolutionary variation in the core RNAi pathway genes are mostly unknown, but may lead to the species-specific functions associated with gene silencing. The two-spotted spider mite, Tetranychus urticae, is a major polyphagous chelicerate pest capable of feeding on over 1100 plant species and developing resistance to pesticides used for its control. A well annotated genome, susceptibility to RNAi and economic importance, make T. urticae an excellent candidate for development of an RNAi protocol that enables high-throughput genetic screens and RNAi-based pest control. Here, we show that the length of the exogenous dsRNA critically determines its processivity and ability to induce RNAi in vivo. A combination of the long dsRNAs and the use of dye to trace the ingestion of dsRNA enabled the identification of genes involved in membrane transport and 26S proteasome degradation as sensitive RNAi targets. Our data demonstrate that environmental RNAi can be an efficient reverse genetics and pest control tool in T. urticae. In addition, the species-specific properties together with the variation in the components of the RNAi machinery make T. urticae a potent experimental system to study the evolution of RNAi pathways.
Marigold flower like structured Cu2NiSnS4 electrode for high energy asymmetric solid state supercapacitors
Authors: Isacfranklin M. , Yuvakkumar R. , Ravi G. , Hong S. I. , Shini Foo , Thambidurai M. , Dang Cuong , Velauthapillai Dhayalan
Published: November 2020

The growth in energy devices and the role of supercapacitors are increasingly important in today’s world. Designing an electrode material for supercapacitors using metals that have high performance, superior structure, are eco-friendly, inexpensive and highly abundant is essentially required for commercialization. In this point of view, quaternary chalcogenide Cu2NiSnS4 with fascinating marigold flower like microstructured electrodes are synthesized using different concentrations of citric acid (0, 0.05 M, 0.1 M and 0.2 M) by employing solvothermal method. The electrode materials physicochemical characteristics are deliberated in detail using the basic characterization techniques. The electrochemical studies revealed better electrochemical performances, in particular, Cu2NiSnS4@0.1 M-CA electrode revealed high 1029 F/g specific capacitance at 0.5 A/g current density. Further, it retained 78.65% capacity over 5000 cycles. To prove the practical applicability, a full-cell asymmetric solid-state device is fabricated, and it delivered 41.25 Wh/Kg and 750 Wh/Kg energy and power density at 0.5 A/g. The optimum citric acid added Cu2NiSnS4 electrode is shown to be a promising candidate for supercapacitor applications.
Aqueous synthesis of highly adsorptive copper–gallic acid metal–organic framework
Authors: Azhar Badril , Angkawijaya Artik Elisa , Santoso Shella Permatasari , Gunarto Chintya , Ayucitra Aning , Go Alchris Woo , Tran-Nguyen Phuong Lan , Ismadji Suryadi , Ju Yi-Hsu
Published: November 2020

A greener route to synthesize mesoporous copper–gallic acid metal–organic framework (CuGA MOF) than the conventional method using harmful DMF solvent was proposed in this study. Various synthesis attempts were conducted by modifying the synthesis conditions to produce CuGA MOF with comparable physical properties to a reference material (DMF-synthesized CuGA NMOF). The independent variables investigated include the molar ratio of NaOH to GA (1.1 to 4.4) and the synthesis temperature (30, 60, 90 °C). It was found that proper NaOH addition was crucial for suppressing the generation of copper oxide while maximizing the formation of CuGA MOF. On the other hand, the reaction temperature mainly affected the stability and adsorption potential of CuGA MOF. Reacting Cu, GA, and NaOH at a molar ratio of 1:1:2.2 and a temperature of 90 °C, produced mesoporous MOF (CuGA 90–2.2) with a surface area of 198.22 m2/g, a pore diameter of 8.6 nm, and a thermal stability of 219 °C. This MOF exhibited an excellent adsorption capacity for the removal of methylene blue (124.64 mg/g) and congo red (344.54 mg/g). The potential usage of CuGA 90–2.2 as a reusable adsorbent was demonstrated by its high adsorption efficiency (> 90%) after 5 adsorption–desorption cycles.
Non-classical crystallisation pathway directly observed for a pharmaceutical crystal via liquid phase electron microscopy
Authors: Cookman J. , Hamilton V. , Hall S. R. , Bangert U.
Published: November 2020

Non-classical crystallisation (NCC) pathways are widely accepted, however there is conflicting evidence regarding the intermediate stages of crystallisation, how they manifest and further develop into crystals. Evidence from direct observations is especially lacking for small organic molecules, as distinguishing these low-electron dense entities from their similar liquid-phase surroundings presents signal-to-noise ratio and contrast challenges. Here, Liquid Phase Electron Microscopy (LPEM) captures the intermediate pre-crystalline stages of a small organic molecule, flufenamic acid (FFA), a common pharmaceutical. High temporospatial imaging of FFA in its native environment, an organic solvent, suggests that in this system a Pre-Nucleation Cluster (PNC) pathway is followed by features exhibiting two-step nucleation. This work adds to the growing body of evidence that suggests nucleation pathways are likely an amalgamation of multiple existing non-classical theories and highlights the need for the direct evidence presented by in situ techniques such as LPEM.
Establishment of a heart-on-a-chip microdevice based on human iPS cells for the evaluation of human heart tissue function
Authors: Abulaiti Mosha , Yalikun Yaxiaer , Murata Kozue , Sato Asako , Sami Mustafa M. , Sasaki Yuko , Fujiwara Yasue , Minatoya Kenji , Shiba Yuji , Tanaka Yo , Masumoto Hidetoshi
Published: November 2020

Human iPS cell (iPSC)-derived cardiomyocytes (CMs) hold promise for drug discovery for heart diseases and cardiac toxicity tests. To utilize human iPSC-derived CMs, the establishment of three-dimensional (3D) heart tissues from iPSC-derived CMs and other heart cells, and a sensitive bioassay system to depict physiological heart function are anticipated. We have developed a heart-on-a-chip microdevice (HMD) as a novel system consisting of dynamic culture-based 3D cardiac microtissues derived from human iPSCs and microelectromechanical system (MEMS)-based microfluidic chips. The HMDs could visualize the kinetics of cardiac microtissue pulsations by monitoring particle displacement, which enabled us to quantify the physiological parameters, including fluidic output, pressure, and force. The HMDs demonstrated a strong correlation between particle displacement and the frequency of external electrical stimulation. The transition patterns were validated by a previously reported versatile video-based system to evaluate contractile function. The patterns are also consistent with oscillations of intracellular calcium ion concentration of CMs, which is a fundamental biological component of CM contraction. The HMDs showed a pharmacological response to isoproterenol, a β-adrenoceptor agonist, that resulted in a strong correlation between beating rate and particle displacement. Thus, we have validated the basic performance of HMDs as a resource for human iPSC-based pharmacological investigations.
The antibacterial and angiogenic effect of magnesium oxide in a hydroxyapatite bone substitute
Authors: Coelho Catarina C. , Padrão Tatiana , Costa Laura , Pinto Marta T . , Costa Paulo C. , Domingues Valentina F. , Quadros Paulo A. , Monteiro Fernando J. , Sousa Susana R.
Published: November 2020

Bone graft infections are serious complications in orthopaedics and the growing resistance to antibiotics is increasing the need for antibacterial strategies. The use of magnesium oxide (MgO) is an interesting alternative since it possesses broad-spectrum antibacterial activity. Additionally, magnesium ions also play a role in bone regeneration, which makes MgO more appealing than other metal oxides. Therefore, a bone substitute composed of hydroxyapatite and MgO (HAp/MgO) spherical granules was developed using different sintering heat-treatment cycles to optimize its features. Depending on the sintering temperature, HAp/MgO spherical granules exhibited distinct surface topographies, mechanical strength and degradation profiles, that influenced the in vitro antibacterial activity and cytocompatibility. A proper balance between antibacterial activity and cytocompatibility was achieved with HAp/MgO spherical granules sintered at 1100 ºC. The presence of MgO in these granules was able to significantly reduce bacterial proliferation and simultaneously provide a suitable environment for osteoblasts growth. The angiogenic and inflammation potentials were also assessed using the in vivo chicken embryo chorioallantoic membrane (CAM) model and the spherical granules containing MgO stimulated angiogenesis without increasing inflammation. The outcomes of this study evidence a dual effect of MgO for bone regenerative applications making this material a promising antibacterial bone substitute.
Klein paradox for bosons, wave packets and negative tunnelling times
Authors: Gutiérrez de la Cal X. , Alkhateeb M. , Pons M. , Matzkin A. , Sokolovski D.
Published: November 2020

We analyse a little known aspect of the Klein paradox. A Klein–Gordon boson appears to be able to cross a supercritical rectangular barrier without being reflected, while spending there a negative amount of time. The transmission mechanism is demonstrably acausal, yet an attempt to construct the corresponding causal solution of the Klein–Gordon equation fails. We relate the causal solution to a divergent multiple-reflections series, and show that the problem is remedied for a smooth barrier, where pair production at the energy equal to a half of the barrier’s height is enhanced yet remains finite.
Genome skimming and NMR chemical fingerprinting provide quality assurance biotechnology to validate Sarsaparilla identity and purity
Authors: Kesanakurti Prasad , Thirugnanasambandam Arunachalam , Ragupathy Subramanyam , Newmaster Steven G.
Published: November 2020

Sarsaparilla is a popular natural health product (NHP) that has been reported to be one of the most adulterated botanicals in the marketplace. Several plausible explanations are documented including economically motivated product substitution, unintentional errors due to ambiguous trade name associated with several different taxa, and wild harvesting of incorrect non-commercial plants. Unfortunately, this includes the case of an adulterant speciesDecalepis hamiltonii,a Red listed medicinal plant species by the International Union for Conservation of Nature (IUCN) and declared as a species with high conservation concern by the National Biodiversity Authority of India (NBA). This study provides validated genomic (genome skimming & DNA probes) and metabolomic (NMR chemical fingerprints) biotechnology solutions to prevent adulteration on both raw materials and finished products. This is also the first use of Oxford Nanopore on herbal products enabling the use of genome skimming as a tool for quality assurance within the supply chain of botanical ingredients. The validation of both genomics and metabolomics approach provided quality assurance perspective for both product identity and purity. This research enables manufactures and retailers to verify their supply chain is authentic and that consumers can enjoy safe, healthy products.