bluuebunny/crossref_metadata_2025 huggingface dataset:
Nanoformulations-Based Metronomic Chemotherapy: Mechanism, Challenges, Recent Advances, and Future Perspectives
Authors: Panthi Vijay Kumar , Dua Kamal , Singh Sachin Kumar , Gupta Gaurav , Hansbro Philip M. , Paudel Keshav Raj
Published: April 2023

Cancer-related death is a significant health and economic burden worldwide, and some conventional chemotherapy is associated with limited effectiveness in completely curing various cancers, severe adverse effects, and destruction of healthy cells. To overcome the complications associated with conventional treatment, metronomic chemotherapy (MCT) is extensively suggested. In this review, we aim to highlight the importance of MCT over conventional chemotherapeutic approach with emphasis on nanoformulations-based MCT, their mechanism, challenges, recent advances, and future perspectives. Nanoformulations-based MCT revealed remarkable antitumor activity in both preclinical and clinical settings. For example, the metronomic scheduling of oxaliplatin-loaded nanoemulsion and polyethylene glycol-coated stealth nanoparticles incorporating paclitaxel were proven very effective in tumor-bearing mice and rats, respectively. Additionally, several clinical studies have demonstrated the benefit of MCT with acceptable tolerance. Moreover, metronomic might be a promising treatment strategy for improving cancer care in low- and middle-income nations. However, an appropriate alternative to a metronomic regimen for an individual ailment, suitable combinational delivery and scheduling, and predictive biomarkers are certain parts that remain unanswered. Further clinical-based comparative research studies are mandatory to be performed before entailing this treatment modality in clinical practice as alternative maintenance therapy or in place of transferring to therapeutic management.
Versatile Solid Modifications of Multicomponent Pharmaceutical Salts: Novel Metformin–Rhein Salts Based on Advantage Complementary Strategy Design
Authors: Yu Mingchao , Liang Meidai , An Qi , Wang Wenwen , Zhang Baoxi , Yang Shiying , Zhou Jian , Yang Xiuying , Yang Dezhi , Zhang Li , Du Guanhua , Lu Yang
Published: April 2023

This study aimed to develop an effective treatment for diabetes and diabetic complications, based on the advantage complementary strategy of drug–drug salt, by designing and synthesizing the multicomponent molecular salts containing metformin (MET) and rhein (RHE). Finally, the salts of MET–RHE (1:1), MET–RHE–H2O (1:1:1), MET–RHE–ethanol–H2O (1:1:1:1), and MET–RHE–acetonitrile (2:2:1) were obtained, indicating the polymorphism of salts formed by MET and RHE. The structures were analyzed by the combination of characterization experiments and theoretical calculation, and the formation mechanism of polymorphism was discussed. The obtained results of in vitro evaluation showed that MET–RHE had a similar hygroscopicity with metformin hydrochloride (MET·HCl), and the solubility of the component of RHE increased by approximately 93 times, which laid a foundation for improving the bioavailability of MET and RHE in vivo. The evaluation of hypoglycemic activity in mice (C57BL/6N) indicated that MET–RHE exhibited better hypoglycemic activity than the parent drugs and the physical mixtures of MET and RHE. The above findings demonstrate that this study achieved the complementary advantages of MET and RHE through the multicomponent pharmaceutical salification technique, and provides new possibilities for the treatment of diabetic complications.
Recombinant Proteins for Assembling as Nano- and Micro-Scale Materials for Drug Delivery: A Host Comparative Overview
Authors: Corchero José Luis , Favaro Marianna T. P. , Márquez-Martínez Merce , Lascorz Jara , Martínez-Torró Carlos , Sánchez Julieta M. , López-Laguna Hèctor , de Souza Ferreira Luís Carlos , Vázquez Esther , Ferrer-Miralles Neus , Villaverde Antonio , Parladé Eloi
Published: April 2023

By following simple protein engineering steps, recombinant proteins with promising applications in the field of drug delivery can be assembled in the form of functional materials of increasing complexity, either as nanoparticles or nanoparticle-leaking secretory microparticles. Among the suitable strategies for protein assembly, the use of histidine-rich tags in combination with coordinating divalent cations allows the construction of both categories of material out of pure polypeptide samples. Such molecular crosslinking results in chemically homogeneous protein particles with a defined composition, a fact that offers soft regulatory routes towards clinical applications for nanostructured protein-only drugs or for protein-based drug vehicles. Successes in the fabrication and final performance of these materials are expected, irrespective of the protein source. However, this fact has not yet been fully explored and confirmed. By taking the antigenic RBD domain of the SARS-CoV-2 spike glycoprotein as a model building block, we investigated the production of nanoparticles and secretory microparticles out of the versions of recombinant RBD produced by bacteria (Escherichia coli), insect cells (Sf9), and two different mammalian cell lines (namely HEK 293F and Expi293F). Although both functional nanoparticles and secretory microparticles were effectively generated in all cases, the technological and biological idiosyncrasy of each type of cell factory impacted the biophysical properties of the products. Therefore, the selection of a protein biofabrication platform is not irrelevant but instead is a significant factor in the upstream pipeline of protein assembly into supramolecular, complex, and functional materials.
Levofloxacin HCl-Incorporated Zein-Based Solvent Removal Phase Inversion In Situ Forming Gel for Periodontitis Treatment
Authors: Senarat Setthapong , Rojviriya Catleya , Puyathorn Napaphol , Lertsuphotvanit Nutdanai , Phaechamud Thawatchai
Published: April 2023

Zein is composed of nonpolar amino acids and is a water-insoluble protein used as the matrix-forming agent of localized in situ forming gel (ISG). Therefore, this study prepared solvent removal phase inversion zein-based ISG formulations to load levofloxacin HCl (Lv) for periodontitis treatment using dimethyl sulfoxide (DMSO) and glycerol formal (GF) as the solvents. Their physicochemical properties were determined, including viscosity, injectability, gel formation, and drug release. The topography of dried remnants after drug release was revealed using a scanning electron microscope and X-ray computed microtomography (μCT) to investigate their 3D structure and % porosity. The antimicrobial activities were tested against Staphylococcus aureus (ATCC 6538), Escherichia coli ATCC 8739, Candida albicans ATCC 10231, and Porphyromonas gingivalis ATCC 33277 with agar cup diffusion. Increasing zein concentration or using GF as the solvent notably enhanced the apparent viscosity and injection force of the zein ISG. However, its gel formation slowed due to the dense zein matrix barrier’s solvent exchange: the higher loaded zein or utilization of GF as an ISG solvent prolonged Lv release. The SEM and μCT images revealed the scaffold of dried ISG in that their % porosity corresponded with their phase transformation and drug release behavior. In addition, the sustainability of drug diffusion promoted a smaller antimicrobial inhibition clear zone. Drug release from all formulations was attained with minimum inhibitory concentrations against pathogen microbes and exhibited a controlled release over 7 days. Lv-loaded 20% zein ISG using GF as a solvent exhibited appropriate viscosity, Newtonian flow, acceptable gel formation and injectability, and prolonged Lv release over 7 days with efficient antimicrobial activities against various test microbes; thus, it is the potential ISG formulation for periodontitis treatment. Consequently, the Lv-loaded solvent removal zein-based ISGs proposed in this investigation offer promising potential as an efficacious drug delivery system for periodontitis treatment by local injection.
Innovative Strategies for Hair Regrowth and Skin Visualization
Authors: Mai Qiuying , Han Yanhua , Cheng Guopan , Ma Rui , Yan Zhao , Chen Xiaojia , Yu Guangtao , Chen Tongkai , Zhang Shu
Published: April 2023

Today, about 50% of men and 15–30% of women are estimated to face hair-related problems, which create a significant psychological burden. Conventional treatments, including drug therapy and transplantation, remain the main strategies for the clinical management of these problems. However, these treatments are hindered by challenges such as drug-induced adverse effects and poor drug penetration due to the skin’s barrier. Therefore, various efforts have been undertaken to enhance drug permeation based on the mechanisms of hair regrowth. Notably, understanding the delivery and diffusion of topically administered drugs is essential in hair loss research. This review focuses on the advancement of transdermal strategies for hair regrowth, particularly those involving external stimulation and regeneration (topical administration) as well as microneedles (transdermal delivery). Furthermore, it also describes the natural products that have become alternative agents to prevent hair loss. In addition, given that skin visualization is necessary for hair regrowth as it provides information on drug localization within the skin’s structure, this review also discusses skin visualization strategies. Finally, it details the relevant patents and clinical trials in these areas. Together, this review highlights the innovative strategies for skin visualization and hair regrowth, aiming to provide novel ideas to researchers studying hair regrowth in the future.
Pediococcus acidilactici pA1c® Improves the Beneficial Effects of Metformin Treatment in Type 2 Diabetes by Controlling Glycaemia and Modulating Intestinal Microbiota
Authors: Cabello-Olmo Miriam , Oneca María , Urtasun Raquel , Pajares María J. , Goñi Saioa , Riezu-Boj José I. , Milagro Fermín I. , Ayo Josune , Encio Ignacio J. , Barajas Miguel , Araña Miriam
Published: April 2023

Type 2 diabetes (T2D) is a complex metabolic disease, which involves maintained hyperglycemia, mainly due to the development of an insulin resistance process. Metformin administration is the most prescribed treatment for diabetic patients. In a previously published study, we demonstrated that Pediococcus acidilactici pA1c® (pA1c) protects from insulin resistance and body weight gain in HFD-induced diabetic mice. The present work aimed to evaluate the possible beneficial impact of a 16-week administration of pA1c, metformin, or the combination of pA1c and metformin in a T2D HFD-induced mice model. We found that the simultaneous administration of both products attenuated hyperglycemia, increased high-intensity insulin-positive areas in the pancreas and HOMA-β, decreased HOMA-IR and also provided more beneficial effects than metformin treatment (regarding HOMA-IR, serum C-peptide level, liver steatosis or hepatic Fasn expression), and pA1c treatment (regarding body weight or hepatic G6pase expression). The three treatments had a significant impact on fecal microbiota and led to differential composition of commensal bacterial populations. In conclusion, our findings suggest that P. acidilactici pA1c® administration improved metformin beneficial effects as a T2D treatment, and it would be a valuable therapeutic strategy to treat T2D.
Phase-Derived Ranging Based Fiber Transfer Delay Measurement Using a Composite Signal for Distributed Radars with Fiber Networks
Authors: Sun Qiang , Liu Chenyu , Yang Jiyao , Liu Jianwei , Dong Jingwen , Li Wangzhe
Published: April 2023

Fiber transfer delay (FTD) variations influence the coherence of distributed radars with fiber networks, resulting in a performance degradation in target detecting and imaging. To measure and compensate for the variation, a phase-derived ranging based FTD measurement using a composite signal is proposed. The composite signal comprises a sinusoidal component and a linear frequency modulation (LFM) component. As the composite signal passes through a fiber under test (FUT), the sinusoidal component generates a phase shift that corresponds to the FTD. The phase shift can be represented by two parameters: the number of complete periods of 2π that can be estimated by using the LFM component, and a phase shift less than 2π that be measured employing the sinusoidal component. When using the proposed measurement system to measure FTD variations in a distributed radar, only an additional sinusoidal component is needed, which minimizes interference with radar signals. Moreover, the proposed measurement system can share core function modules such as signal generation and process modules with distributed radars, which enhances the compatibility and reduces the overall complexity. Experiments are carried out to measure a variable optical delay line and a long optical fiber. The experiment results verify the feasibility of the measurement system and show that a measurement range of more than 15 km, an accuracy of ±0.1 ps and a measurement time of 105 ms can be achieved.
Optical and Mechanical Performance and Feasibility Analysis of Meter-Level Corrector Lenses for Survey Telescope
Authors: Cheng Xue , Liu Changhua
Published: April 2023

A prime-focus optical system is a common type of large aperture survey telescope with a small obscuration ratio, high transmittance, and simple structure. As the detection capabilities and search efficiency of the telescope are improved, the aperture of the primary mirror becomes larger. The primary mirror aperture of a high-performance survey telescope has a four-meter scale, and the aperture of the corrector lenses of a corresponding prime-focus optical system can reach a one-meter scale. In this study, optical surface shape changes due to gravity were studied via an integrated optomechanical analysis. The Zernike polynomial was used to characterize the changed optical surface. The influence of optical surface variations on the wavefront and imaging quality of the optical system was analyzed by calculating the optical path differences. From the perspective of optical aberration, the optical properties of meter-level corrector lenses were quantitatively analyzed. The effect of the meter-level corrector lenses on imaging was explained in principle, and the feasibility of this optical scheme was verified.
Effect of HIFU-Induced Thermal Ablation in Numerical Breast Phantom
Authors: Yadav Sumit Kumar , Paul Souradip , Singh Mayanglambam Suheshkumar
Published: April 2023

Breast cancer is a leading cause of cancer-related deaths in women, and treatment involved invasive surgery such as lumpectomy. In the last decade, a non-invasive, non-contact high-intensity focused ultrasound (HIFU) therapy was developed for treatment with promising results. However, its success rate depends on patient selection, tissue heterogeneities, HIFU operational parameters, and even imaging techniques. In this emerging field, computer simulations can provide us with a much-needed platform to learn, test, and deduce results virtually before conducting experiments. In this study, we used three different classes of anatomically realistic numerical breast phantoms from clinical contrast-enhanced magnetic resonance imaging (MRI) data, including scattered-, heterogeneous-, and extremely dense-type breasts. Upon assigning the appropriate acoustic and optical parameters to the tissues within, we simulated HIFU propagation by using the k-Wave toolbox in MATLAB and compared the changes introduced in the three types of breasts. It was found that scattered-type breast was best-suited for HIFU therapy. Furthermore, we simulated light-beam propagation with the ValoMC toolbox in MATLAB after introducing the lesion to compare the distribution of the initial pressure generated via the photoacoustic effect. This simulation study will be of significant clinical impact, especially in the study and management of HIFU-based treatments, which are individual/tissue-selective in nature.
Wideband Airy Beam Generation Using Reflective Metasurfaces with Both Phase and Amplitude Modulation
Authors: Wang Zhenai , Lang Tingting , Qiu Yanqing
Published: April 2023

Airy beams are an intriguing type of non-diffraction wave packet that can exist in one-dimensional (1D) curved orbital plane systems. These beams have gained significant attention due to their unique properties, including non-diffraction, self-healing, and self-bending. In this study, we propose a method for generating high-efficiency and wideband Airy beams in microwave sections with reflective geometry, which is challenging to achieve due to the complexity of the Airy beam’s function and generation structure. Our approach involves both phase modulation and amplitude modulation. The designed metasurfaces were numerically simulated to obtain the most significant features of the microwave Airy beam. In addition, they are well in agreement with those predicted by theory.
Jambu Flower Extract (Acmella oleracea) Increases the Antioxidant Potential of Beer with a Reduced Alcohol Content
Authors: da Silva Suelem Paixão , Fernandes José Augusto Lacerda , Santos Alberdan Silva , Ferreira Nelson Rosa
Published: April 2023

Craft beers with different sensory perceptions have received the attention of more demanding consumers. In this sense, the application of plant extracts as brewing adjuncts is being increasingly studied. Allied with these perspectives is the consumption of lower alcoholic beverages, which also represents the desire for a market niche that has been growing gradually. The present work aimed to produce craft lager beer with the addition of plant extract and reduced alcohol content by partial replacement of malt with malt bagasse. The physical-chemical analyzes of the beer produced showed that it was possible to reduce the alcohol content by 40.5% compared to the control sample. In addition, an extract of Acmella oleracea (Jambu) obtained by supercritical extraction was added to increase the beer’s antioxidant capacity. The ABTS, DPPH, and ORAC methods evaluated the antioxidant capacity. These assays were performed again after six months of storage. The quantification and identification of the significant substance in the extract (spilanthol) were performed using Gas Chromatography (GC-FID), Thin Layer Chromatography (TLC), and Attenuated Total Reflectance Infrared Spectroscopy (FTIR-ATR). The results showed significant increases in antioxidant activity compared to the sample without extract. This positive aspect opens a perspective for using jambu flower extract as a prominent antioxidant adjunct in beer.
Antibiotic-Potentiating Activity of the Schinus terebinthifolius Raddi Essential Oil against MDR Bacterial Strains
Authors: Costa da Silva Maria Milene , Bezerra de Araújo Neto José , Lucas dos Santos Antonia Thassya , de Morais Oliveira-Tintino Cícera Datiane , de Araújo Ana Carolina Justino , Freitas Priscilla Ramos , da Silva Luiz Everson , do Amaral Wanderlei , Deschamps Cícero , de Azevedo Francisco Roberto , Gonçalves Lima Clara Mariana , Golubkina Nadezhda , Calixto-Júnior João Tavares , Ribeiro-Filho Jaime , Coutinho Henrique Douglas Melo , Caruso Gianluca , Tintino Saulo Relison
Published: April 2023

Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus are the primary bacteria that cause clinical infections, such as urinary and intestinal infections, pneumonia, endocarditis, and sepsis. Bacterial resistance is an innate natural occurrence in microorganisms, resulting from mutations or the lateral exchange of genetic material. This serves as evidence for the association between drug consumption and pathogen resistance. Evidence has demonstrated that the association between conventional antibiotics and natural products is a promising pharmacological strategy to overcome resistance mechanisms. Considering the large body of research demonstrating the significant antimicrobial activities of Schinus terebinthifolius Raddi, the present study aimed to evaluate the chemical composition and antibiotic-enhancing effects of Schinus terebinthifolius Raddi essential oil (STEO) against the standard and multidrug-resistant strains of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The STEO was extracted by hydrodistillation using a Clevenger-type vacuum rotary evaporator. The Minimum Inhibitory Concentration (MIC) of the STEO was assessed by the microdilution method to evaluate the antibacterial activity. The antibiotic-enhancing activity of the essential oil was assessed by determining the MIC of antibiotics in the presence of a sub-inhibitory concentration (MIC/8) of the natural product. The GC-MS analysis revealed alpha-pinene (24.3%), gamma-muurolene (16.6%), and myrcene (13.7%) as major constituents of the STEO. The STEO potentiated the enhanced antibacterial activity of norfloxacin and gentamicin against all the strains and increased the action of penicillin against the Gram-negative strains. Therefore, it is concluded that although the STEO does not exhibit clinically effective antibacterial activity, its association with conventional antibiotics results in enhanced antibiotic activity.
Performance of Plant-Growth-Promoting Rhizobacteria (PGPR) Isolated from Sandy Soil on Growth of Tomato (Solanum lycopersicum L.)
Authors: Rehan Medhat , Al-Turki Ahmad , Abdelmageed Adil H. A. , Abdelhameid Noha M. , Omar Ayman F.
Published: April 2023

The plant-growth-promoting rhizobacteria (PGPR) in the rhizosphere affect plant growth, health, and productivity, as well as soil-nutrient contents. They are considered a green and eco-friendly technology that will reduce chemical-fertilizer usage, thereby reducing production costs and protecting the environment. Out of 58 bacterial strains isolated in Qassim, Saudi Arabia, four strains were identified by the 16S rRNA as the Streptomyces cinereoruber strain P6-4, Priestia megaterium strain P12, Rossellomorea aquimaris strain P22-2, and Pseudomonas plecoglossicida strain P24. The plant-growth-promoting (PGP) features of the identified bacteria involving inorganic phosphate (P) solubilization, the production of indole acetic acid (IAA), and siderophore secretion were assessed in vitro. Regarding the P solubilization, the previous strains’ efficacy reached 37.71%, 52.84%, 94.31%, and 64.20%, respectively. The strains produced considerable amounts of IAA (69.82, 251.70, 236.57, and 101.94 µg/mL) after 4 days of incubation at 30 °C. Furthermore, the rates of siderophore production reached 35.51, 26.37, 26.37, and 23.84 psu, respectively, in the same strains. The application of the selected strains in the presence of rock phosphate (RP) with tomato plants under greenhouse conditions was evaluated. The plant growth and P-uptake traits positively and significantly increased in response to all the bacterial treatments, except for some traits, such as plant height, number of leaves, and leaf DM at 21 DAT, compared to the negative control (rock phosphate, T2). Notably, the P. megaterium strain P12 (T4), followed by R. aquimaris strain P22-2 (T5), revealed the best values related to plant height (at 45 DAT), number of leaves per plant (at 45 DAT), root length, leaf area, leaf-P uptake, stem P uptake, and total plant P uptake compared to the rock phosphate. The first two components of the PCA (principal component analysis) represented 71.99% (PCA1 = 50.81% and PCA2 = 21.18%) of the variation at 45 DAT. Finally, the PGPR improved the vegetative-growth traits of the tomato plants through P solubilization, IAA, and siderophore production, and ameliorated the availability of nutrients. Thus, applying in PGPR in sustainable agriculture will potentially reduce production costs and protect the environment from contamination by chemical fertilizers and pesticides.
Antitumor and Antioxidant Activities of In Vitro Cultivated and Wild-Growing Clinopodium vulgare L. Plants
Authors: Petrova Maria , Dimitrova Lyudmila , Dimitrova Margarita , Denev Petko , Teneva Desislava , Georgieva Ani , Petkova-Kirova Polina , Lazarova Maria , Tasheva Krasimira
Published: April 2023

Clinopodium vulgare L. is a valuable medicinal plant used for its anti-inflammatory, antibacterial and wound-healing properties. The present study describes an efficient protocol for the micropropagation of C. vulgare and compares, for the first time, the chemical content and composition and antitumor and antioxidant activities of extracts from in vitro cultivated and wild-growing plants. The best nutrient medium was found to be Murashige and Skoog (MS) supplemented with 1 mg/L BAP and 0.1 IBA mg/L, yielding on average 6.9 shoots per nodal segment. Flower aqueous extracts from in vitro plants had higher total polyphenol content (29,927.6 ± 592.1 mg/100 g vs. 27,292.8 ± 85.3 mg/100 g) and ORAC antioxidant activity (7281.3 ± 82.9 µmol TE/g vs. 7246.3 ± 62.4 µmol TE/g) compared to the flowers of wild plants. HPLC detected qualitative and quantitative differences in phenolic constituents between the in vitro cultivated and wild-growing plants’ extracts. Rosmarinic acid was the major phenolic constituent, being accumulated mainly in leaves, while neochlorogenic acid was a major compound in the flowers of cultivated plants. Catechin was found only in cultivated plants, but not in wild plants or cultivated plants’ stems. Aqueous extracts of both cultivated and wild plants showed significant in vitro antitumor activity against human HeLa (cervical adenocarcinoma), HT-29 (colorectal adenocarcinoma) and MCF-7 (breast cancer) cell lines. The best cytotoxic activity against most of the cancer cell lines, combined with the least detrimental effects on a non-tumor human keratinocyte cell line (HaCaT), was shown by the leaf (250 µg/mL) and flower (500 µg/mL) extracts of cultivated plants, making cultivated plants a valuable source of bioactive compounds and a suitable candidate for anticancer therapy.
Effects of Multi-Walled Carbon Nanotubes and Nano-Silica on Root Development, Leaf Photosynthesis, Active Oxygen and Nitrogen Metabolism in Maize
Authors: Hao Yubo , Yu Yang , Sun Guangyan , Gong Xiujie , Jiang Yubo , Lv Guoyi , Zhang Yiteng , Li Liang , Zhao Yang , Sun Dan , Gu Wanrong , Qian Chunrong
Published: April 2023

Carbon nanotubes (MWCNTs) and nano-silica (nano-SiO2) are widely used in the field of life science because of their special physical and chemical properties. In this study, the effects of different concentrations of MWCNTs (0 mg·L−1, 200 mg·L−1, 400 mg·L−1, 800 mg·L−1 and 1200 mg·L−1) and nano-SiO2 (0 mg·L−1, 150 mg·L−1, 800 mg·L−1, 1500 mg·L−1 and 2500 mg·L−1) on maize seedling growth and relative mechanisms were explored. The main results are as follows: MWCNTs and nano-SiO2 can promote the growth of maize seedlings, and promote plant height, root length, the dry and fresh weight of seedlings, root–shoot ratio and so on. The ability to accumulate dry matter increased, the relative water content of leaves increased, the electrical conductivity of leaves decreased, the stability of cell membranes improved and the water metabolism ability of maize seedlings increased. The treatment of MWCNTs with 800 mg·L−1 and nano-SiO2 with 1500 mg·L−1 had the best effect on seedling growth. MWCNTs and nano-SiO2 can promote the development of root morphology, increase root length, root surface area, average diameter, root volume and total root tip number and improve root activity, so as to improve the absorption capacity of roots to water and nutrition. After MWCNT and nano-SiO2 treatment, compared with the control, the contents of O2·− and H2O2 decreased, and the damage of reactive oxygen free radicals to cells decreased. MWCNTs and nano-SiO2 can promote the clearance of reactive oxygen species and maintain the complete structure of cells, so as to slow down plant aging. The promoting effect of MWCNTs treated with 800 mg·L−1 and nano-SiO2 treated with 1500 mg·L−1 had the best effect. After treatment with MWCNTs and nano-SiO2, the activities of key photosynthesis enzymes PEPC, Rubisco, NADP-ME, NADP-MDH and PPDK of maize seedlings increased, which promoted the opening of stomata, improved the fixation efficiency of CO2, improved the photosynthetic process of maize plants and promoted plant growth. The promoting effect was the best when the concentration of MWCNTs was 800 mg·L−1 and the concentration of nano-SiO2 was 1500 mg·L−1. MWCNTs and nano-SiO2 can increase the activities of the enzymes GS, GOGAT, GAD and GDH related to nitrogen metabolism in maize leaves and roots, and can increase the content of pyruvate, so as to promote the synthesis of carbohydrates and the utilization of nitrogen and promote plant growth.
Potential Role of Biochar and Silicon in Improving Physio-Biochemical and Yield Characteristics of Borage Plants under Different Irrigation Regimes
Authors: Farouk Saad , AL-Huqail Arwa Abdulkreem , El-Gamal Seham M. A.
Published: April 2023

Silicon (Si) and biochar (Bc) are key signaling conditioners that improve plant metabolic processes and promote drought tolerance. However, the specific role of their integrative application under water restrictions on economical plants is not yet well understood. Two field experiments throughout 2018/2019 and 2019/2020 were conducted to examine the physio-biochemical modifications and yield attributes of borage plants mediated by Bc (9.52 tons ha−1) and/or Si (300 mg L−1) under different irrigation regimes (100, 75, and 50% of crop evapotranspiration). Catalase (CAT) and peroxidase (POD) activity; relative water content, water, and osmotic potential; leaf area per plant and yield attributes; and chlorophyll (Chl) content, Chla/chlorophyllidea (Chlida), and Chlb/Chlidb were considerably reduced within the drought condition. On the other hand, oxidative biomarkers, as well as organic and antioxidant solutes, were increased under drought, associated with membrane dysfunction, superoxide dismutase (SOD) activation, and osmotic adjustment (OA) capacity as well as a hyperaccumulation of porphyrin intermediates. Supplementation of Bc and Si lessens the detrimental impacts of drought on several plant metabolic processes associated with increasing leaf area and yield attributes. Their application under normal or drought conditions significantly elicited the accumulation of organic and antioxidant solutes as well as the activation of antioxidant enzymes, followed by lessening the formation of free radical oxygen and mitigating oxidative injuries. Moreover, their application maintained water status and OA capacity. Si and/or Bc treatment reduced protoporphyrin, magnesium-protoporphyrin, and protochlorophyllide while increasing Chla and Chlb assimilation and boosting the ratio of Chla/Chlida and Chlb/Chlidb, resulting in a rise in leaf area per plant and yield components following these modifications. These findings highlight the significance of Si and/or Bc as (a) stress-signaling molecule(s) in regulating defensive systems in drought-affected borage plants by boosting antioxidant aptitude, regulating water status, and accelerating chlorophyll assimilation, thus leading to increasing leaf area and productivity.
Application of Chitosan-Based Molecularly Imprinted Polymer in Development of Electrochemical Sensor for p-Aminophenol Determination
Authors: Mulyasuryani Ani , Prananto Yuniar Ponco , Fardiyah Qonitah , Widwiastuti Hanandayu , Darjito Darjito
Published: April 2023

Molecularly Imprinted Polymers (MIPs) have specific recognition capabilities and have been widely used for electrochemical sensors with high selectivity. In this study, an electrochemical sensor was developed for the determination of p-aminophenol (p-AP) by modifying the screen-printed carbon electrode (SPCE) with chitosan-based MIP. The MIP was made from p-AP as a template, chitosan (CH) as a base polymer, and glutaraldehyde and sodium tripolyphosphate as the crosslinkers. MIP characterization was conducted based on membrane surface morphology, FT-IR spectrum, and electrochemical properties of the modified SPCE. The results showed that the MIP was able to selectively accumulate analytes on the electrode surface, in which MIP with glutaraldehyde as a crosslinker was able to increase the signal. Under optimum conditions, the anodic peak current from the sensor increased linearly in the range of 0.5–35 µM p-AP concentration, with sensitivity of (3.6 ± 0.1) µA/µM, detection limit (S/N = 3) of (2.1 ± 0.1) µM, and quantification limit of (7.5 ± 0.1) µM. In addition, the developed sensor exhibited high selectivity with an accuracy of (94.11 ± 0.01)%.
Clove Essential Oil Pickering Emulsions Stabilized with Lactoferrin/Fucoidan Complexes: Stability and Rheological Properties
Authors: Xi Xiaohong , Wei Zihao , Xu Yanan , Xue Changhu
Published: April 2023

Although studies have shown that lactoferrin (LF) and fucoidan (FD) can be used to stabilize Pickering emulsions, there have been no studies on the stabilization of Pickering emulsions via the use of LF–FD complexes. In this study, different LF–FD complexes were obtained by adjusting the pH and heating the LF and FD mixture while using different mass ratios, and the properties of the LF–FD complexes were investigated. The results showed that the optimal conditions for preparing the LF–FD complexes were a mass ratio of 1:1 (LF to FD) and a pH of 3.2. Under these conditions, the LF–FD complexes not only had a uniform particle size of 133.27 ± 1.45 nm but also had good thermal stability (the thermal denaturation temperature was 110.3 °C) and wettability (the air-water contact angle was 63.9 ± 1.90°). The concentration of the LF–FD complexes and the ratio of the oil phase influenced the stability and rheological properties of the Pickering emulsion such that both can be adjusted to prepare a Pickering emulsion with good performance. This indicates that LF–FD complexes represent promising applications for Pickering emulsions with adjustable properties.
Disinfection and Isotonic Drinks’ Influence on Hardness and Color Stability of Ethylene-Vinyl-Acetate Copolymer Mouthguards Used in Martial Arts: An In Vitro Study
Authors: Mańka-Malara Katarzyna , Szerszeń Marcin , Górski Bartłomiej , Tanabe Gen , Ueno Toshiaki , Mierzwińska-Nastalska Elżbieta
Published: April 2023

This in vitro study aimed to evaluate the hardness and color change of an ethylene-vinyl-acetate copolymer (EVA) material for mouthguards after exposition to different cleaning agent solutions and isotonic drinks. Four hundred samples were prepared and divided into four equinumerous groups (n = 100), in which there were 25 samples from each color of EVA (red, green, blue and white). The hardness, using the digital durometer, and the color coordinates (CIE L*a*b*), using the digital colorimeter, were measured before the first exposition and after 3 months of exposition to spray disinfection and incubation in the oral cavity temperature, or immersion in isotonic drinks. The values of Shore A hardness (HA) and color change (ΔE—calculated by Euclidean distance) were statistically analyzed using the Kolmogorov–Smirnov test, multiple comparison ANOVA/Kruskal–Wallis and appropriate post-hoc tests. Statistically significant changes in color and hardness between the tested groups were demonstrated after the use of agents predestined for disinfecting the surface of mouthguards on the tested samples. There were no statistically significant differences in color and hardness between the groups immersed in isotonic sport drinks potentially consumed by competitors practicing combat sports using mouthguards. Despite the changes in color and hardness after the use of disinfectants, the deviations were minor and limited to specific colors of the EVA plates. The intake of isotonic drinks practically did not change either the color or the hardness of the samples, regardless of the tested color of the EVA plates.
Proposal and Design of Flexible All-Polymer/CIGS Tandem Solar Cell
Authors: Alanazi Tarek I. , El Sabbagh Mona
Published: April 2023

Tandem solar cells (TSCs) have attracted prodigious attention for their high efficiency, which can surmount the Shockley–Queisser limit for single-junction solar cells. Flexible TSCs are lightweight and cost-effective, and are considered a promising approach for a wide range of applications. In this paper, a numerical model, based on TCAD simulation, is presented to assess the performance of a novel two-terminal (2T) all-polymer/CIGS TSC. To confirm the model, the obtained simulation results were compared with standalone fabricated all-polymer and CIGS single solar cells. Common properties of the polymer and CIGS complementary candidates are their non-toxicity and flexibility. The initial top all-polymer solar cell had a photoactive blend layer (PM7:PIDT), the optical bandgap of which was 1.76 eV, and the initial bottom cell had a photoactive CIGS layer, with a bandgap of 1.15 eV. The simulation was then carried out on the initially connected cells, revealing a power conversion efficiency (PCE) of 16.77%. Next, some optimization techniques were applied to enhance the tandem performance. Upon treating the band alignment, the PCE became 18.57%, while the optimization of polymer and CIGS thicknesses showed the best performance, reflected by a PCE of 22.73%. Moreover, it was found that the condition of current matching did not necessarily meet the maximum PCE condition, signifying the essential role of full optoelectronic simulations. All TCAD simulations were performed via an Atlas device simulator, where the light illumination was AM1.5G. The current study can offer design strategies and effective suggestions for flexible thin-film TSCs for potential applications in wearable electronics.
Design and Characterization of Baricitinib Incorporated PLA 3D Printed Pills by Fused Deposition Modeling: An Oral Pill for Treating Alopecia Areata
Authors: Ahmed Mohammed Muqtader , Fatima Farhat , Alnami Aisha , Alsenaidy Mohammad , Aodah Alhussain H. , Aldawsari Mohammed F. , Almutairy Bjad , Anwer Md. Khalid , Jafar Mohammed
Published: April 2023

This study aimed to develop three-dimensional (3D) baricitinib (BAB) pills using polylactic acid (PLA) by fused deposition modeling. Two strengths of BAB (2 and 4% w/v) were dissolved into the (1:1) PEG-400 individually, diluting it with a solvent blend of acetone and ethanol (27.8:18:2) followed by soaking the unprocessed 200 cm~6157.94 mg PLA filament in the solvent blend acetone—ethanol. FTIR spectrums of the 3DP1 and 3DP2 filaments calculated and recognized drug encapsulation in PLA. Herein, 3D-printed pills showed the amorphousness of infused BAB in the filament, as indicated by DSC thermograms. Fabricated pills shaped like doughnuts increased the surface area and drug diffusion. The releases from 3DP1 and 3DP2 were found to be 43.76 ± 3.34% and 59.14 ± 4.54% for 24 h. The improved dissolution in 3DP2 could be due to the higher loading of BAB due to higher concentration. Both pills followed Korsmeyer–Peppas’ order of drug release. BAB is a novel JAK inhibitor that U.S. FDA has recently approved to treat alopecia areata (AA). Therefore, the proposed 3D printed tablets can be easily fabricated with FDM technology and effectively used in various acute and chronic conditions as personalized medicine at an economical cost.
Mechanical, Thermal, and Fire Retardant Properties of Rice Husk Biochar Reinforced Recycled High-Density Polyethylene Composite Material
Authors: Shah Atta ur Rehman , Imdad Anas , Sadiq Atiya , Malik Rizwan Ahmed , Alrobei Hussein , Badruddin Irfan Anjum
Published: April 2023

This study concentrated on the influence of rice husk biochar on the structural, thermal, flammable, and mechanical properties of recycled high-density polyethylene (HDPE). The percentage of rice husk biochar with recycled HDPE was varied between 10% and 40%, and the optimum percentages were found for the various properties. Mechanical characteristics were evaluated in terms of the tensile, flexural, and impact properties. Similarly, the flame retardancy of the composites was observed by means of horizontal and vertical burning tests (UL-94 tests), limited oxygen index, and cone calorimetry. The thermal properties were characterized using thermogravimetric analysis (TGA). For detailed characterization, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) tests were performed, to elaborate on the variation in properties. The composite with 30% rice husk biochar demonstrated the maximum increase in tensile and flexural strength, i.e., 24% and 19%, respectively, compared to the recycled HDPE, whereas the 40% composite showed a 22.5% decrease in impact strength. Thermogravimetric analysis revealed that the 40% rice husk biochar reinforced composite exhibited the best thermal stability, due to having the highest amount of biochar. In addition, the 40% composite also displayed the lowest burning rate in the horizontal burning test and the lowest V-1 rating in the vertical burning test. The 40% composite material also showed the highest limited oxygen index (LOI), whereas it had the lowest peak heat release rate (PHRR) value (52.40% reduced) and total heat release rate (THR) value (52.88% reduced) for cone calorimetry, when compared with the recycled HDPE. These tests proved that rice husk biochar is a significant additive for enhancing the mechanical, thermal, and fire-retardant properties of recycled HDPE.
Dynamic Processes and Mechanical Properties of Lipid–Nanoparticle Mixtures
Authors: Pan Fan , Sun Lingling , Li Shiben
Published: April 2023

In this study, we investigate the dynamic processes and mechanical properties of lipid nanoparticle mixtures in a melt via dissipation particle dynamic simulation. By investigating the distribution of nanoparticles in lamellar and hexagonal lipid matrices in equilibrium state and dynamic processes, we observe that the morphology of such composites depends not only on the geometric features of the lipid matrix but also on the concentration of nanoparticles. The dynamic processes are also demonstrated by calculating the average radius of gyration, which indicates the isotropic conformation of lipid molecules in the x–y plane and that the lipid chains are stretched in the z direction with the addition of nanoparticles. Meanwhile, we predict the mechanical properties of lipid–nanoparticle mixtures in lamellar structures by analyzing the interfacial tensions. Results show that the interfacial tension decreased with the increase in nanoparticle concentration. These results provide molecular-level information for the rational and a priori design of new lipid nanocomposites with ad hoc tailored properties.
Atomistic Simulation of Flow-Induced Microphase Separation and Crystallization of an Entangled Polyethylene Melt Undergoing Uniaxial Elongational Flow and the Role of Kuhn Segment Extension
Authors: Nafar Sefiddashti Mohammad Hadi , Edwards Brian J. , Khomami Bamin
Published: April 2023

Atomistic simulations of the linear, entangled polyethylene C1000H2002 melt undergoing steady-state and startup conditions of uniaxial elongational flow (UEF) over a wide range of flow strength were performed using a united-atom model for the atomic interactions between the methylene groups constituting the polymer macromolecules. Rheological, topological, and microstructural properties of these nonequilibrium viscoelastic materials were computed as functions of strain rate, focusing on regions of flow strength where flow-induced phase separation and flow-induced crystallization were evident. Results of the UEF simulations were compared with those of prior simulations of planar elongational flow, which revealed that uniaxial and planar flows exhibited essentially a universal behavior, although over strain rate ranges that were not completely equivalent. At intermediate flow strength, a purely configurational microphase separation was evident that manifested as a bicontinuous phase composed of regions of highly stretched molecules that enmeshed spheroidal domains of relatively coiled chains. At high flow strength, a flow-induced crystallization (FIC) occurred, producing a semicrystalline material possessing a high degree of crystallinity and primarily a monoclinic lattice structure. This FIC phase formed at a temperature (450 K) high above the quiescent melting point (≈400 K) and remained stable after cessation of flow for temperature at or below 435 K. Careful examination of the Kuhn segments constituting the polymer chains revealed that the FIC phase only formed once the Kuhn segments had become essentially fully extended under the UEF flow field. Thermodynamic properties such as the heat of fusion and heat capacity were estimated from the simulations and found to compare favorably with experimental values.
Enthalpic Determination of the Interaction of Modified Activated Carbons with Benzene and Hexane as Pure Solvents and Binary Mixtures
Authors: Hernández-Monje Diana , Giraldo Liliana , Serafin Jarosław , Moreno-Piraján Juan Carlos
Published: April 2023

Three activated carbons with different physicochemical characteristics are prepared and their N2 isotherms at 77 K and CO2 isotherms at 273 K are determined. The energetic interaction between three activated carbons with different physicochemical properties, and two hydrocarbons as pure liquids and as binary mixtures at different concentrations, was characterized by determining the enthalpy of immersion, in order to evaluate the effect of adsorbents and adsorbates, as well as that of the addition of another adsorbate to the system. For the pure solvents, the enthalpy of immersion (ΔHi) is higher for the aromatic compound (−94.98 to −128.80 J g−1) than for the aliphatic compound (−16.36 to −53.35 J g−1); for the mixtures, the values are between −36.39 and −98.37 J g−1, where the interaction increases with the solid that was subjected to thermal treatment and presented the lowest content of surface oxygenated groups, while the energetic parameter decreases with the solid that presents chemical modification with nitric acid, behavior that is evident in the pure solvents and in the mixtures. Activated carbons have a CO2 adsorption between 3.43 and 3.79 mmol g−1.
Study on Mechanism and Verification of Columnar Penetration Grouting of Time-Varying Newtonian Fluids
Authors: Zhao Xuguang , Yang Zhiquan , Meng Xiangrui , Wang Shaobin , Li Rui , Xu Hanhua , Wang Xiangpeng , Ye Changwen , Xiang Tianbing , Xu Wanzhong , Chen Junzhi , Wang Ping , Yuan Jinke , Li Xiaohui
Published: April 2023

Penetration grouting technology is an important technical means to improve the mechanical properties of gravel soil layers, and the time-varying characteristics of Newtonian fluid viscosity have an important influence on the morphology and effect of penetration grouting. However, these time-varying properties are not considered in the current research on the mechanism of Newtonian fluid penetration grouting. In this paper, by studying the basic rheological equation of Newtonian fluids and its dynamic viscosity time-varying law, the penetration motion equation of viscosity time-varying Newtonian fluids is discussed, by means of theoretical analysis and experimental research. Based on this, the time-varying viscosity Newtonian fluid columnar penetration grouting diffusion mechanism (TVNCPGDM) equation is derived, the application scope of the equation is analyzed and a grouting experiment is designed to verify it. The results show that the theoretical value of the grouting diffusion radius calculated by the TVNCPGDM equation, is closer to the experimental value than that obtained by the equation of columnar penetration grouting without considering the viscosity time-varying Newtonian fluid, with a 12.9% improvement in accuracy. This shows that the TVNCPGDM equation derived in this paper, can better reflect the diffusion law and diffusion morphology of column penetration grouting of Newtonian fluid, which changes with time in the injected medium; and the diffusion radius obtained for penetration grouting is more in line with the actual grouting engineering demands. The research results can provide some theoretical guidance for the actual grouting of loose gravel soil layers.