bluuebunny/crossref_metadata_2025 huggingface dataset:
Towards Extending Dividend Puzzle Debate: What Motivates Distribution of Corporate Earnings in Tanzania?
Authors: Lotto Josephat
Published: March 2020

This paper investigates the determinants of dividend policy in Tanzania. The study employed a panel data of non-financial firms listed on the Dar es Salaam Stock Exchange (DSE) for the period 2008–2017. The paper reports profitability, liquidity, firm size, leverage, firm growth, previous dividend, and GDP as the major determinants of corporate dividend policy. According to the results, leverage, firm growth, and GDP are negatively related to dividend payout ratio while firm size, profitability, liquidity, and lagged dividend are positively related to dividend policy. More specifically, large-sized firms, highly profitable firms, and firms who paid dividend in previous years are more likely to consider paying dividend. However, payment of dividend will all depend on whether the firm is liquid enough to afford that. On the other hand, high-growth and leveraged firms would not probably consider paying dividend, and will, therefore, opt saving money to finance their expansion and honor their debt obligations. Following these results, corporate managers are advised to consider preferences of investors towards developing corporate dividend policy; to strive paying dividend whenever economically viable (as it signals the firm’s reputation), and to limit excessive borrowing to protect firms from getting into financial meltdown (although borrowing is considered a control tool for agency-related problems).
Spatio-Temporal Analysis of Intense Convective Storms Tracks in a Densely Urbanized Italian Basin
Authors: Sangiorgio Matteo , Barindelli Stefano
Published: March 2020

Intense convective storms usually produce large rainfall volumes in short time periods, increasing the risk of floods and causing damages to population, buildings, and infrastructures. In this paper, we propose a framework to couple visual and statistical analyses of convective thunderstorms at the basin scale, considering both the spatial and temporal dimensions of the process. The dataset analyzed in this paper contains intense convective events that occurred in seven years (2012–2018) in the Seveso-Olona-Lambro basin (North of Italy). The data has been acquired by MeteoSwiss using the Thunderstorm Radar Tracking (TRT) algorithm. The results show that the most favorable conditions for the formation of convective events occur in the early afternoon and during summertime, confirming the key role of the temperature in atmospheric convection. The orography emerged as a driver for convection, which takes place more frequently in mountain areas. The storm paths analysis shows that the predominant direction is from South-West to North-East. Considering storm duration, long-lasting events reach higher values of radar reflectivity and cover more extended areas than short-lasting ones. The results obtained can be exploited for many practical applications including nowcasting, alert systems, and sensors deployment.
Uber Movement Data: A Proxy for Average One-way Commuting Times by Car
Authors: Sun Yeran , Ren Yinming , Sun Xuan
Published: March 2020

Recently, Uber released datasets named Uber Movement to the public in support of urban planning and transportation planning. To prevent user privacy issues, Uber aggregates car GPS traces into small areas. After aggregating car GPS traces into small areas, Uber releases free data products that indicate the average travel times of Uber cars between two small areas. The average travel times of Uber cars in the morning peak time periods on weekdays could be used as a proxy for average one-way car-based commuting times. In this study, to demonstrate usefulness of Uber Movement data, we use Uber Movement data as a proxy for commuting time data by which commuters’ average one-way commuting time across Greater Boston can be figured out. We propose a new approach to estimate the average car-based commuting times through combining commuting times from Uber Movement data and commuting flows from travel survey data. To further demonstrate the applicability of the commuting times estimated by Uber movement data, this study further measures the spatial accessibility of jobs by car by aggregating place-to-place commuting times to census tracts. The empirical results further uncover that 1) commuters’ average one-way commuting time is around 20 min across Greater Boston; 2) more than 75% of car-based commuters are likely to have a one-way commuting time of less than 30 min; 3) less than 1% of car-based commuters are likely to have a one-way commuting time of more than 60 min; and 4) the areas suffering a lower level of spatial accessibility of jobs by car are likely to be evenly distributed across Greater Boston.
Genome-Wide Analysis of Phosphorus Transporter Genes in Brassica and Their Roles in Heavy Metal Stress Tolerance
Authors: Wan Yuanyuan , Wang Zhen , Xia Jichun , Shen Shulin , Guan Mingwei , Zhu Meichen , Qiao Cailin , Sun Fujun , Liang Ying , Li Jiana , Lu Kun , Qu Cunmin
Published: March 2020

Phosphorus transporter (PHT) genes encode H2PO4−/H+ co-transporters that absorb and transport inorganic nutrient elements required for plant development and growth and protect plants from heavy metal stress. However, little is known about the roles of PHTs in Brassica compared to Arabidopsis thaliana. In this study, we identified and extensively analyzed 336 PHTs from three diploid (B. rapa, B. oleracea, and B. nigra) and two allotetraploid (B. juncea and B. napus) Brassica species. We categorized the PHTs into five phylogenetic clusters (PHT1–PHT5), including 201 PHT1 homologs, 15 PHT2 homologs, 40 PHT3 homologs, 54 PHT4 homologs, and 26 PHT5 homologs, which are unevenly distributed on the corresponding chromosomes of the five Brassica species. All PHT family genes from Brassica are more closely related to Arabidopsis PHTs in the same vs. other clusters, suggesting they are highly conserved and have similar functions. Duplication and synteny analysis revealed that segmental and tandem duplications led to the expansion of the PHT gene family during the process of polyploidization and that members of this family have undergone purifying selection during evolution based on Ka/Ks values. Finally, we explored the expression profiles of BnaPHT family genes in specific tissues, at various developmental stages, and under heavy metal stress via RNA-seq analysis and qRT-PCR. BnaPHTs that were induced by heavy metal treatment might mediate the response of rapeseed to this important stress. This study represents the first genome-wide analysis of PHT family genes in Brassica species. Our findings improve our understanding of PHT family genes and provide a basis for further studies of BnaPHTs in plant tolerance to heavy metal stress.
Microgravity and Cell Adherence
Authors: Bauer Johann
Published: March 2020

Cell adhesion is an inevitable precondition for enabling cells to assemble into three-dimensional tissues [...]
DNA Methylation in Neurodegenerative and Cerebrovascular Disorders
Authors: Martínez-Iglesias Olaia , Carrera Iván , Carril Juan Carlos , Fernández-Novoa Lucía , Cacabelos Natalia , Cacabelos Ramón
Published: March 2020

DNA methylation is an epigenetic mechanism by which methyl groups are added to DNA, playing a crucial role in gene expression regulation. The aim of the present study is to compare methylation status of healthy subjects with that of patients with Alzheimer’s, Parkinson’s or Cerebrovascular diseases. We also analyze methylation status of a transgenic Alzheimer’s disease mouse model (3xTg-AD). Our results show that both global methylation (n = 141) and hydroxymethylation (n = 131) levels are reduced in DNA samples from buffy coats of patients with neurodegenerative disorders and age-related cerebrovascular disease. The importance of methylation and hydroxymethylation reduction is stressed by the finding that DNMT3a mRNA levels are also downregulated in buffy coats of patients with Dementia (n = 25). Global methylation is also reduced in brain, liver and serum samples of 3xTg-AD vs. wild type mice, such as DNMT3a mRNA levels that are also decreased in the brain of 3xTg-AD (n = 10). These results suggest that the use of global methylation and hydroxymethylation levels, together with the study of DNMT3a expression, could be useful as a new diagnostic biomarker for these prevalent disorders.
Structures of Human Transglutaminase 2: Finding Clues for Interference in Cross-linking Mediated Activity
Authors: Kim Gi Eob , Park Hyun Ho
Published: March 2020

Human transglutaminase 2 (TGase2) has various functions, including roles in various cellular processes such as apoptosis, development, differentiation, wound healing, and angiogenesis, and is linked to many diseases such as cancer. Although TGase2 has been considered an optimized drug target for the treatment of cancer, fibrosis, and neurodegenerative disorders, it has been difficult to generate TGase2-targeted drugs for clinical use because of the relatively flat and broad active site on TGase2. To design more specific and powerful inhibitors, detailed structural information about TGase2 complexed with various effector and inhibitor molecules is required. In this review, we summarized the current structural studies on TGase2, which will aid in designing drugs that can overcome the aforementioned limitations.
Light and Circadian Signaling Pathway in Pregnancy: Programming of Adult Health and Disease
Authors: Hsu Chien-Ning , Tain You-Lin
Published: March 2020

Light is a crucial environmental signal that affects elements of human health, including the entrainment of circadian rhythms. A suboptimal environment during pregnancy can increase the risk of offspring developing a wide range of chronic diseases in later life. Circadian rhythm disruption in pregnant women may have deleterious consequences for their progeny. In the modern world, maternal chronodisruption can be caused by shift work, jet travel across time zones, mistimed eating, and excessive artificial light exposure at night. However, the impact of maternal chronodisruption on the developmental programming of various chronic diseases remains largely unknown. In this review, we outline the impact of light, the circadian clock, and circadian signaling pathways in pregnancy and fetal development. Additionally, we show how to induce maternal chronodisruption in animal models, examine emerging research demonstrating long-term negative implications for offspring health following maternal chronodisruption, and summarize current evidence related to light and circadian signaling pathway targeted therapies in pregnancy to prevent the development of chronic diseases in offspring.
SIRT2 Affects Primary Cilia Formation by Regulating mTOR Signaling in Retinal Pigmented Epithelial Cells
Authors: Lim Jeaho , Son Juyoung , Ryu Jaewook , Kim Ja-Eun
Published: March 2020

SIRT2, a member of the Class III HDAC family, participates in diverse cellular processes and regulates several pathological conditions. Although a few reports show that SIRT2 regulates the cell cycle, the causes and outcomes of SIRT2-dependent cell proliferation remain unclear. Here, we examined the effects of SIRT2 suppression in human RPE1 cells using siRNA targeting SIRT2, and AK-1, a SIRT2-specific inhibitor. The number of primary cilia in SIRT2-suppressed cells increased under serum-present conditions. Suppressing SIRT2 induced cell cycle arrest at G0/G1 phase by inactivating mammalian target of rapamycin (mTOR) signaling, possibly through mTORC1. Treatment with torin 1, an inhibitor of mTORC1/mTORC2, yielded results similar to those observed after SIRT2 suppression. However, SIRT2 suppression did not affect primary cilia formation or mTOR signaling following serum starvation. This suggests that SIRT2 acts as a critical sensor that links growth factor-dependent signal transduction and primary cilia formation by regulating the cell cycle.
Lineage Decision-Making within Normal Haematopoietic and Leukemic Stem Cells
Authors: Brown Geoffrey , Sánchez Lucía , Sánchez-García Isidro
Published: March 2020

To produce the wide range of blood and immune cell types, haematopoietic stem cells can “choose” directly from the entire spectrum of blood cell fate-options. Affiliation to a single cell lineage can occur at the level of the haematopoietic stem cell and these cells are therefore a mixture of some pluripotent cells and many cells with lineage signatures. Even so, haematopoietic stem cells and their progeny that have chosen a particular fate can still “change their mind” and adopt a different developmental pathway. Many of the leukaemias arise in haematopoietic stem cells with the bulk of the often partially differentiated leukaemia cells belonging to just one cell type. We argue that the reason for this is that an oncogenic insult to the genome “hard wires” leukaemia stem cells, either through development or at some stage, to one cell lineage. Unlike normal haematopoietic stem cells, oncogene-transformed leukaemia stem cells and their progeny are unable to adopt an alternative pathway.
Cancer Diagnosis through SERS and Other Related Techniques
Authors: Blanco-Formoso Maria , Alvarez-Puebla Ramon A.
Published: March 2020

Cancer heterogeneity increasingly requires ultrasensitive techniques that allow early diagnosis for personalized treatment. In addition, they should preferably be non-invasive tools that do not damage surrounding tissues or contribute to body toxicity. In this context, liquid biopsy of biological samples such as urine, blood, or saliva represents an ideal approximation of what is happening in real time in the affected tissues. Plasmonic nanoparticles are emerging as an alternative or complement to current diagnostic techniques, being able to detect and quantify novel biomarkers such as specific peptides and proteins, microRNA, circulating tumor DNA and cells, and exosomes. Here, we review the latest ideas focusing on the use of plasmonic nanoparticles in coded and label-free surface-enhanced Raman scattering (SERS) spectroscopy. Moreover, surface plasmon resonance (SPR) spectroscopy, colorimetric assays, dynamic light scattering (DLS) spectroscopy, mass spectrometry or total internal reflection fluorescence (TIRF) microscopy among others are briefly examined in order to highlight the potential and versatility of plasmonics.
Attacker Behaviour Forecasting Using Methods of Intelligent Data Analysis: A Comparative Review and Prospects
Authors: Doynikova Elena , Novikova Evgenia , Kotenko Igor
Published: March 2020

Early detection of the security incidents and correct forecasting of the attack development is the basis for the efficient and timely response to cyber threats. The development of the attack depends on future steps available to the attackers, their goals, and their motivation—that is, the attacker “profile” that defines the malefactor behaviour in the system. Usually, the “attacker profile” is a set of attacker’s attributes—both inner such as motives and skills, and external such as existing financial support and tools used. The definition of the attacker’s profile allows determining the type of the malefactor and the complexity of the countermeasures, and may significantly simplify the attacker attribution process when investigating security incidents. The goal of the paper is to analyze existing techniques of the attacker’s behaviour, the attacker’ profile specifications, and their application for the forecasting of the attack future steps. The implemented analysis allowed outlining the main advantages and limitations of the approaches to attack forecasting and attacker’s profile constructing, existing challenges, and prospects in the area. The approach for attack forecasting implementation is suggested that specifies further research steps and is the basis for the development of an attacker behaviour forecasting technique.
Standardized Test Procedure for External Human–Machine Interfaces of Automated Vehicles
Authors: Kaß Christina , Schoch Stefanie , Naujoks Frederik , Hergeth Sebastian , Keinath Andreas , Neukum Alexandra
Published: March 2020

Research on external human–machine interfaces (eHMIs) has recently become a major area of interest in the field of human factors research on automated driving. The broad variety of methodological approaches renders the current state of research inconclusive and comparisons between interface designs impossible. To date, there are no standardized test procedures to evaluate and compare different design variants of eHMIs with each other and with interactions without eHMIs. This article presents a standardized test procedure that enables the effective usability evaluation of eHMI design solutions. First, the test procedure provides a methodological approach to deduce relevant use cases for the evaluation of an eHMI. In addition, we define specific usability requirements that must be fulfilled by an eHMI to be effective, efficient, and satisfying. To prove whether an eHMI meets the defined requirements, we have developed a test protocol for the empirical evaluation of an eHMI with a participant study. The article elucidates underlying considerations and details of the test protocol that serves as framework to measure the behavior and subjective evaluations of non-automated road users when interacting with automated vehicles in an experimental setting. The standardized test procedure provides a useful framework for researchers and practitioners.
Organizational Culture as an Indication of Readiness to Implement Industry 4.0
Authors: Ziaei Nafchi Majid , Mohelská Hana
Published: March 2020

Industry 4.0 has managed to attract lots of attention from researchers because of the benefits it has to offer and many studies about Industry 4.0, as well as readiness and maturity models, are available from the technological point of view. Meanwhile, the organizational culture dimension of Industry 4.0 has received little to no attention. The aim of this paper is to find out whether or not the size and type of an organization influence the innovative culture and consequently the readiness of the organization for implementing industry 4.0. Results show that the innovative organizational culture according to the index of organizational culture does not depend on the size of an organization but to some degree depends on the type of the organization.
Photophysical and Electrocatalytic Properties of Rhenium(I) Triazole-Based Complexes
Authors: Comia Adrian , Charalambou Luke , Omar Salem A. E. , Scattergood Paul A. , Elliott Paul I. P. , Sinopoli Alessandro
Published: March 2020

A series of [Re(N^N)(CO)3(Cl)] (N^N = diimine) complexes based on 4-(pyrid-2-yl)-1,2,3-triazole (1), 1-benzyl-4-(pyrimidin-2-yl)-1,2,3-triazole (2), and 1-benzyl-4-(pyrazin-2-yl)-1,2,3-triazole (3) diimine ligands were prepared and their photophysical and electrochemical properties were characterized. The ligand-based reduction wave is shown to be highly sensitive to the nature of the triazole-based ligand, with the peak potential shifting by up to 600 mV toward more positive potential from 1 to 3. All three complexes are phosphorescent in solution at room temperature with λmax ranging from 540 nm (1) to 638 nm (3). Interestingly, the complexes appear to show inverted energy-gap law behaviour (τ = 43 ns for 1 versus 92 ns for 3), which is tentatively interpreted as reduced thermal accessibility of metal-centred (3MC) states from photoexcited metal to ligand charge transfer (3MLCT) states upon stabilisation of the N^N-centred lowest unoccupied molecular orbital (LUMO). The photophysical characterisation, supported by computational data, demonstrated a progressive stabilization of the LUMO from complex 1 to 3, which results in a narrowing of the HOMO–LUMO energy gap (HOMO = highest occupied molecular orbital) across the series and, correspondingly, red-shifted electronic absorption and photoluminescence spectra. The two complexes bearing pyridyl (1) and pyrimidyl (2) moieties, respectively, showed a modest ability to catalyse the electroreduction of CO2, with a peak potential at ca. −2.3 V versus Fc/Fc+. The catalytic wave that is observed in the cyclic voltammograms is slightly enhanced by the addition of water as a proton source.
Blood Digestion by Trypsin-Like Serine Proteases in the Replete Lyme Disease Vector Tick, Ixodes scapularis
Authors: Reyes Jeremiah , Ayala-Chavez Cuauhtemoc , Sharma Arvind , Pham Michael , Nuss Andrew B. , Gulia-Nuss Monika
Published: March 2020

Ixodes scapularis is the major vector of Lyme disease in the Eastern United States. Each active life stage (larva, nymph, and adult) takes a blood meal either for developing and molting to the next stage (larvae and nymphs) or for oviposition (adult females). This protein-rich blood meal is the only food taken by Ixodes ticks and therefore efficient blood digestion is critical for survival. Studies in partially engorged ticks have shown that the initial stages of digestion are carried out by cathepsin proteases within acidic digestive cells. In this study, we investigated the potential role of serine proteases in blood digestion in replete ticks. RNA interference was used for functional analysis and a trypsin-benzoyl-D, L-arginine 4-nitoanilide assay was used to measure active trypsin levels. Hemoglobinolytic activity was determined in vitro, with or without a serine protease inhibitor. Our data suggest that trypsin levels increase significantly after repletion. Knockdown of serine proteases negatively impacted blood feeding, survival, fecundity, levels of active trypsin in the midgut, and resulted in lower hemoglobin degradation. Incubation of midgut extract with a trypsin inhibitor resulted in 65% lower hemoglobin degradation. We provide evidence of the serine proteases as digestive enzymes in fully engorged, replete females. Understanding the digestive profile of trypsin during blood meal digestion in I. scapularis improves our understanding of the basic biology of ticks and may lead to new methods for tick control.
Effects of a Multicomponent Exercise Program in Older Adults with Non-Small-Cell Lung Cancer during Adjuvant/Palliative Treatment: An Intervention Study
Authors: Rosero Ilem D. , Ramírez-Vélez Robinson , Martínez-Velilla Nicolas , Cedeño-Veloz Bernardo Abel , Morilla Idoia , Izquierdo Mikel
Published: March 2020

Clinical intervention studies support the efficacy and safety of exercise programs as a treatment modality for non-small-cell lung cancer (NSCLC) during adjuvant/palliative treatment, but the effectiveness of real-world oncogeriatric services is yet to be established. We aimed to examine the effects of a 10-week structured and individualized multicomponent exercise program on physical/cognitive functioning and mental wellness in elderly patients with NSCLC under adjuvant therapy or palliative treatment. A non-randomized, opportunistic control, longitudinal-design trial was conducted on 26 patients with NSCLC stage I–IV. Of 34 eligible participants, 21 were allocated into two groups: (i) control group (n = 7) received usual medical care; and (ii) intervention group (n =19) received multicomponent program sessions, including endurance, strength, balance, coordination and stretching exercises. Tests included the Short Physical Performance Battery (SPPB), 5-m habitual Gait Velocity Test (GVT), Timed Up & Go Test (TUG), 6-Min Walk Test (6MWT), independence of activities in daily living (IADL), muscular performance, cognitive function, and quality of life, which were measured at baseline and after 10 weeks of the program. Results revealed a significant group×time interaction for SPPB (p = 0.004), 5-m GVT (p = 0.036), TUG (p = 0.007), and muscular performance (chest and leg power; p < 0.001). Similarly, significant changes were observed between groups for cognitive functioning (p = 0.021) and quality of life for EUROQoL 5D (p = 0.006). Our findings confirm that a multicomponent exercise program improves measures of physical/cognitive functioning and quality of life in the elderly with NSCLC under adjuvant therapy or palliative treatment. This is an interesting and important study that adds to our current body of knowledge on the safety of exercise interventions, especially in the elderly with solid tumors.
Effect of Botulinum Toxin A on Scar Healing after Thyroidectomy: A Prospective Double-blind Randomized Controlled Trial
Authors: Bae Dong Sik , Koo Do Hoon , Kim Ji Eun , Cho Jae-mahn , Park Jun-Ook
Published: March 2020

The persistence of neck scarring is a common concern among patients undergoing thyroidectomy. Botulinum toxin A (BTA (Botox)) has been shown to suppress scar enlargement at the incision site. The objective of this study was to evaluate the effect of intraoperative Botox administration on neck scarring after thyroidectomy. A prospective double-blind randomized clinical trial was performed in patients undergoing conventional thyroidectomy. Forty patients were randomly allocated to a Botox or a control group (both, n = 20). The wound was closed after injection into the platysma muscle of 50 U of Botox diluted in 1 mL of normal saline or 1 mL of saline alone. Skin scars were assessed using the modified Stony Brook Scar Evaluation Scale (SBSES) and Manchester Scar Scale (MSS) at 1, 12, and 24 weeks postoperatively. The SBSES and MSS scores of the Botox group were significantly better than those of the control group (p = 0.034 and p = 0.039). At 24 weeks postoperatively, the SBSES and MSS scores were significantly better in the Botox group (p = 0.006 and p = 0.030). BTA injected into the incision site can suppress postoperative scar formation and thereby improve the cosmetic outcome.
Emergent Properties of the HNF4α-PPARγ Network May Drive Consequent Phenotypic Plasticity in NAFLD
Authors: Sahoo Sarthak , Singh Divyoj , Chakraborty Priyanka , Jolly Mohit Kumar
Published: March 2020

Non-alcoholic fatty liver disease (NAFLD) is the most common form of chronic liver disease in adults and children. It is characterized by excessive accumulation of lipids in the hepatocytes of patients without any excess alcohol intake. With a global presence of 24% and limited therapeutic options, the disease burden of NAFLD is increasing. Thus, it becomes imperative to attempt to understand the dynamics of disease progression at a systems-level. Here, we decoded the emergent dynamics of underlying gene regulatory networks that were identified to drive the initiation and the progression of NAFLD. We developed a mathematical model to elucidate the dynamics of the HNF4α-PPARγ gene regulatory network. Our simulations reveal that this network can enable multiple co-existing phenotypes under certain biological conditions: an adipocyte, a hepatocyte, and a “hybrid” adipocyte-like state of the hepatocyte. These phenotypes may also switch among each other, thus enabling phenotypic plasticity and consequently leading to simultaneous deregulation of the levels of molecules that maintain a hepatic identity and/or facilitate a partial or complete acquisition of adipocytic traits. These predicted trends are supported by the analysis of clinical data, further substantiating the putative role of phenotypic plasticity in driving NAFLD. Our results unravel how the emergent dynamics of underlying regulatory networks can promote phenotypic plasticity, thereby propelling the clinically observed changes in gene expression often associated with NAFLD.
Assessment of the Short-Term Effects after High-Inductive Electromagnetic Stimulation of Pelvic Floor Muscles: A Randomized, Sham-Controlled Study
Authors: Ptaszkowski Kuba , Malkiewicz Bartosz , Zdrojowy Romuald , Ptaszkowska Lucyna , Paprocka-Borowicz Malgorzata
Published: March 2020

Background: Physiotherapy should be performed by patients with stress or mixed urinary incontinence (SUI and MUI) to increase the strength and endurance of the pelvic floor muscles (PFMs). A method that can positively affect the pelvic floor is stimulation with high-inductive electromagnetic stimulation (HIES). The aim of the study was to evaluate the PFMs after the application of HIES in women with SUI and MUI by using surface electromyography (sEMG). Methods: This was a prospective, randomized, single-blind study with a sham intervention group. The participants were randomly assigned to the HIES group or sham group. The outcomes were features of the bioelectrical PFM activity assessed using sEMG and endovaginal probes. A single-session intervention in the HIES group included 20 min of HIES with an electromagnetic induction intensity of 2.5 T. Results: In the HIES group, there was a statistically significant difference in the PFM sEMG activity during “contractions” (p < 0.001) and “quick flicks” (p = 0.005). In the intergroup comparison, higher PFM sEMG activity after the intervention (“contraction”) was observed in the HIES group than in the sham group (after: p = 0.047; 1 h after: p = 0.017). Conclusions: The assessed HIES method seems effective for SUI and MUI patients in the short term and shows an advantage over the sham intervention in the assessment of PFM contractions.
Kidney Perfusion as an Organ Quality Assessment Tool—Are We Counting Our Chickens Before They Have Hatched?
Authors: De Beule Julie , Jochmans Ina
Published: March 2020

The final decision to accept an organ for transplantation remains a subjective one. With “poor organ quality” commonly cited as a major reason for kidney discard, accurate, objective, and reliable quality assessment is essential. In an era of increasingly higher-risk deceased donor kidneys, the catch is to accept those where the risk–benefit scale will tip in the right direction. Currently available assessment tools, such as risk-scores predicting outcome and zero-time biopsy, perform unsatisfactory, and assessment options during static cold storage are limited. Kidney perfusion technologies are finding their way into clinical practice, and they bring a new opportunity to assess kidney graft viability and quality, both in hypothermic and normothermic conditions. We give an overview of the current understanding of kidney viability assessment during ex situ kidney perfusion. A pragmatic framework to approach viability assessment is proposed as an interplay of three different compartments: the nephron, the vascular compartment, and the immune compartment. Although many interesting ways to assess kidney injury and function during perfusion have been proposed, none have reached the stage where they can reliably predict posttransplant outcome. Larger well-designed studies and validation cohorts are needed to provide better guidance.
Special Issue: Sleep Bruxism—The Controversial Sleep Movement Activity
Authors: Wieckiewicz Mieszko , Winocur Efraim
Published: March 2020

According to the current approach [1], bruxism is considered as two different behaviours observed during sleep and wakefulness, respectively, and the single definition for bruxism has been replaced by two separate definitions: Sleep bruxism is a masticatory muscle activity during sleep that is characterised as rhythmic (phasic) or non-rhythmic (tonic) and is not a movement disorder or a sleep disorder in otherwise healthy individuals [...]
Delayed Reconstruction of Palatomaxillary Defect Using Fibula Free Flap
Authors: Byun Soo-Hwan , Lim Ho-Kyung , Yang Byoung-Eun , Kim Soung-Min , Lee Jong-Ho
Published: March 2020

Introduction. The objective of this study was to evaluate a surgical technique and to present the results of delayed reconstruction of palatomaxillary defects using fibula free flap (FFF). Methods. A review was conducted for nine patients who underwent palatomaxillary reconstruction using FFF. Primary disease, type of reconstruction, defect area, fibula segment length and number of osteotomies, radiotherapy, and implant installation after FFF reconstruction were analyzed. Results. All nine patients underwent delayed reconstruction. The fibula shaft was osteotomized into two segments in seven patients and three segments in one patient with bilateral Brown’s revised classification IV/d defect. One case was planned by using a computer-aided design computer-aided manufacturing (CAD/CAM) system with a navigation system. The mean length of the grafted fibula bone was 68.06 mm. Dental implant treatment was performed in six patients. Six patients received radiation therapy, and there were no specific complications related to the radiation therapy. In one case, the defect was reconstructed with FFF flow-through from a radial forearm free flap. Conclusion. This clinical study demonstrated that the fibula flap is an ideal donor-free flap in a palatomaxillary defect. Delayed reconstruction using an FFF can reduce the complication and failure rates.
Metabolomic Analysis Reveals Distinct Profiles in the Plasma and Urine Associated with IgE Reactions in Childhood Asthma
Authors: Chiu Chih-Yung , Cheng Mei-Ling , Chiang Meng-Han , Wang Chia-Jung , Tsai Ming-Han , Lin Gigin
Published: March 2020

Several metabolomics studies have identified altered metabolic pathways that are related to asthma. However, an integrative analysis of the metabolic responses across blood and urine for a comprehensive framework of asthma in early childhood remains lacking. Fifty-four age-matched children with asthma (n = 28) and healthy controls (n = 26) were enrolled. Metabolome analysis of the plasma and urine samples was performed using 1H-nuclear magnetic resonance (NMR) spectroscopy coupled with partial least-squares discriminant analysis (PLS-DA). Integrated analysis of blood and urine metabolic profiling related to IgE reactions for childhood asthma was investigated. A significantly higher plasma histidine level was found, in parallel with lower urinary 1-methylnicotinamide and trimethylamine N-oxide (TMAO) levels, in children with asthma compared to healthy controls. Compared to children without allergic sensitization, 11 (92%) plasma metabolites and 8 (80%) urinary metabolites were found to be significantly different in children with IgE and food sensitization respectively. There were significant correlations between the plasma 3-hydroxybutyric acid and excreted volumes of the hydroxy acids, which were strongly correlated to plasma leucine and valine levels. Urine N-phenylacetylglycine, a microbial-host co-metabolite, was strongly correlated with total serum and food allergen-specific IgE levels. Plasma pyruvate and urine valine, leucine, and isoleucine degradation metabolisms were significantly associated with allergic sensitization for childhood asthma. In conclusion, blood and urine metabolome reflect different metabolic pathways in allergic reactions. Plasma pyruvate metabolism to acetic acid appears to be associated with serum IgE production, whereas urine branched-chain amino acid metabolism primarily reflects food allergic reactions against allergies.
Tc-99m TRODAT-1 SPECT is a Potential Biomarker for Restless Leg Syndrome in Patients with End-Stage Renal Disease
Authors: Hou Yi-Chou , Fan Yu-Ming , Huang Ya-Ching , Chen Ruei-Ming , Wang Cheng-Hsu , Lin Yi-Te , Yen Tzung-Hai , Lu Kuo-Cheng , Lin Yuh-Feng
Published: March 2020

Rationales: Restless leg syndrome (RLS) is a common complication in patients with end-stage renal disease (ESRD). However, there is a lack of biomarkers linking uremic RLS to dopaminergic neurons. Previous studies demonstrated that Tc-99m TRODAT-1 SPECT was a biomarker for RLS but the correlation between the physiologic parameter was lacking. Methods: Overall, 32 patients were enrolled in the study and divided into the following 3 groups: (1) control (n = 13), (2) ESRD without RLS (n = 8) and (3) ESRD with RLS (n = 11). All patients had a clinical diagnosis of RLS and received Tc-99m TRODAT-1 SPECT. A subgroup analysis was performed to compare differences between the control and ESRD with RLS groups. Tc-99m TRODAT-1 SPECT was performed and activities in the striatum and occipital areas were measured using manually delineated regions of interest (ROIs) by an experienced nuclear medicine radiologist who was blinded to clinical data. Results: The total ratio of Tc-99m TRODAT SPECT was lower in the ESRD with RLS group (p = 0.046). The uptake ratio of TRODAT negatively correlated with serum parathyroid hormone (r = −0.577, p = 0.015) and ferritin (r = −0.464, p = 0.039) concentrations. However, the uptake positively correlated with the hemoglobin concentration (r = 0.531, p = 0.011). The sensitivity and specificity of the total TRODAT ratio for predicting RLS in the overall population were 95.0% and 67.7%, respectively, at a cutoff value of 0.980 (area under the curve of receiver operating characteristic curve was 0.767, p = 0.024). Conclusion: In patients with ESRD and RLS, Tc-99m TRODAT might be a potential biomarker. Dysregulated hemoglobin, serum parathyroid hormone and serum ferritin concentrations might influence the uptake of the TRODAT ratio.
Semi-Automatic Tool for Vitiligo Detection and Analysis
Authors: Neri Paolo , Fiaschi Michela , Menchini Giovanni
Published: March 2020

Vitiligo vulgaris is an autoimmune disease which causes a strong reduction of the cells producing melanin, which is the main skin pigment. This results in the growth of white patches on patients’ skin, which are more or less visible, depending on the skin phototype. Precise, objective and fast detection of vitiligo patches would be crucial to produce statistically relevant data on huge populations, thus giving an insight on the disease. However, few methods are available in literature. In the present paper, a semi-automatic tool based on image processing to detect facial vitiligo patches is described. The tool requires pictures to be captured under black light illumination, which enhances patches contrast with respect to healthy skin. The user is only required to roughly define the regions of interest and set a global threshold, thus, no specific image-processing skills are required. An adaptive algorithm then automatically discerns between vitiligo and healthy skin pixels. The tools also allow for a statistical data interpretation by overlapping the detected patches of all patients on a face template through an occurrence map. Preliminary results obtained on a small population of 15 patients allowed us to assess the tool’s performance. Patch detection was checked by an experienced dermatologist, who confirmed the detection for all the studied patients, thus supporting the effectiveness of the proposed tool.
Performance Assessment of a Semi-Circular Breakwater through CFD Modelling
Authors: Gomes Ana , Pinho José L. S. , Valente Tiago , Antunes do Carmo José S. , V. Hegde Arkal
Published: March 2020

Coastal defence works, such as breakwaters, are structures that aim to support the action of waves and dissipate their energy. Therefore, they provide conditions for stabilizing the coast, protecting ports, beaches and other coastal infrastructures and ecosystems. Semicircular breakwaters have been applied in different locations around the world due to their aesthetic advantages and high structural performance. Marine structures are subject to hydrodynamic actions normally estimated through physical models. However, these models are complex to implement, involving high costs and long experimental procedures. Thus, alternative methodologies for studying the hydrodynamic performance of these structures are of great use. This work presents the results of the application of a computational fluid dynamics (CFD) tool to study the stability of a perforated semicircular breakwater, based on a rubble mound foundation. The model was validated against experimental results of the critical weight necessary to resist sliding, taking into account the effects of water depth and different characteristics of the waves. A comparison is made between the perforated and the non-perforated solution in terms of the breakwater’s performance to dissipate wave energy. Dissipation conditions of this energy, in the exposed face, are also evaluated in detail, in order to assess the potential of this structure as a biological refuge for marine species. Both solutions show similar performance in terms of results obtained for the wave reflectivity coefficient. The turbulence dissipation on the exposed face of the perforated breakwater is limited to a region of restricted extension around it, which is advantageous in terms of the passage of species into the breakwater.
Utilization of Hydroxyl-Methyl Butyrate, Leucine, Glutamine and Arginine Supplementation in Nutritional Management of Sarcopenia—Implications and Clinical Considerations for Type 2 Diabetes Mellitus Risk Modulation
Authors: Maykish Adeline , Sikalidis Angelos K.
Published: March 2020

While onset characteristics may vary, sarcopenia gradually develops over time as a result of the aging process, leading to muscle loss, disturbance of the muscle to fat ratio, and a variety of negative symptoms undermining the wellbeing, quality of life, and lifespan in the aging population globally. There is evidence that sarcopenia may be a cause and consequence of type 2 diabetes mellitus (T2DM) in the aging population. The importance of nutritional management in the prevention and/or deceleration of sarcopenia is critical, with the main focus placed on the amount and quality of protein intake. Significant efforts are being made towards the development of medical nutrition therapies involving certain amino acids and amino compounds, as well as their combinations, for the improvement in muscle strength, muscle function and protein synthesis. This may reduce hospitalization times and hasten the recovery of patients with sarcopenia. The administration of protocols with varying dose and frequencies, as well as their efficacy, is being investigated. In the work herein, we present and evaluate data derived from human trials regarding the utilization of hydroxyl-methyl butyrate (HMB), L-leucine (Leu), L-glutamine (Gln) and L-arginine (Arg) supplementation for optimal management of sarcopenia in geriatric patients, a topic of significant clinical nutrition interest which may have important implications in T2DM status.
Support Vector Machine Methods and Artificial Neural Networks Used for the Development of Bankruptcy Prediction Models and their Comparison
Authors: Horak Jakub , Vrbka Jaromir , Suler Petr
Published: March 2020

Bankruptcy prediction is always a topical issue. The activities of all business entities are directly or indirectly affected by various external and internal factors that may influence a company in insolvency and lead to bankruptcy. It is important to find a suitable tool to assess the future development of any company in the market. The objective of this paper is to create a model for predicting potential bankruptcy of companies using suitable classification methods, namely Support Vector Machine and artificial neural networks, and to evaluate the results of the methods used. The data (balance sheets and profit and loss accounts) of industrial companies operating in the Czech Republic for the last 5 marketing years were used. For the application of classification methods, TIBCO’s Statistica software, version 13, is used. In total, 6 models were created and subsequently compared with each other, while the most successful one applicable in practice is the model determined by the neural structure 2.MLP 22-9-2. The model of Support Vector Machine shows a relatively high accuracy, but it is not applicable in the structure of correct classifications.
Tough and Low Friction Polyvinyl Alcohol Hydrogels Loaded with Anti-inflammatories for Cartilage Replacement
Authors: Oliveira Andreia Sofia , Schweizer Sara , Nolasco Pedro , Barahona Isabel , Saraiva Jorge , Colaço Rogério , Serro Ana Paula
Published: March 2020

The development of new materials that mimic cartilage and its function is an unmet need that will allow replacing the damaged parts of the joints, instead of the whole joint. Polyvinyl alcohol (PVA) hydrogels have raised special interest for this application due to their biocompatibility, high swelling capacity and chemical stability. In this work, the effect of post-processing treatments (annealing, high hydrostatic pressure (HHP) and gamma-radiation) on the performance of PVA gels obtained by cast-drying was investigated and, their ability to be used as delivery vehicles of the anti-inflammatories diclofenac or ketorolac was evaluated. HHP damaged the hydrogels, breaking some bonds in the polymeric matrix, and therefore led to poor mechanical and tribological properties. The remaining treatments, in general, improved the performance of the materials, increasing their crystallinity. Annealing at 150 °C generated the best mechanical and tribological results: higher resistance to compressive and tensile loads, lower friction coefficients and ability to support higher loads in sliding movement. This material was loaded with the anti-inflammatories, both without and with vitamin E (Vit.E) or Vit.E + cetalkonium chloride (CKC). Vit.E + CKC helped to control the release of the drugs which occurred in 24 h. The material did not induce irritability or cytotoxicity and, therefore, shows high potential to be used in cartilage replacement with a therapeutic effect in the immediate postoperative period.
Pneumatically-Actuated Acoustic Metamaterials Based on Helmholtz Resonators
Authors: Hedayati Reza , Lakshmanan Sandhya
Published: March 2020

Metamaterials are periodic structures which offer physical properties not found in nature. Particularly, acoustic metamaterials can manipulate sound and elastic waves both spatially and spectrally in unpreceded ways. Acoustic metamaterials can generate arbitrary acoustic bandgaps by scattering sound waves, which is a superior property for insulation properties. In this study, one dimension of the resonators (depth of cavity) was altered by means of a pneumatic actuation system. To this end, metamaterial slabs were additively manufactured and connected to a proportional pressure control unit. The noise reduction performance of active acoustic metamaterials in closed- and open-space configurations was measured in different control conditions. The pneumatic actuation system was used to vary the pressure behind pistons inside each cell of the metamaterial, and as a result to vary the cavity depth of each unit cell. Two pressures were considered, P = 0.05 bar, which led to higher depth of the cavities, and P = 0.15 bar, which resulted in lower depth of cavities. The results showed that by changing the pressure from P = 0.05 (high cavity depth) to P = 0.15 (low cavity depth), the acoustic bandgap can be shifted from a frequency band of 150–350 Hz to a frequency band of 300–600 Hz. The pneumatically-actuated acoustical metamaterial gave a peak attenuation of 20 dB (at 500 Hz) in the closed system and 15 dB (at 500 Hz) in the open system. A step forward would be to tune different unit cells of the metamaterial with different pressure levels (and therefore different cavity depths) in order to target a broader range of frequencies.
Synergistic Effects of Graphene/Carbon Nanotubes Hybrid Coating on the Interfacial and Mechanical Properties of Fiber Composites
Authors: Qin Wenzhen , Chen Chao , Zhou Jianping , Meng Jiangyan
Published: March 2020

In this study, the graphene nanoplates (GnPs) and carbon nanotubes (CNTs) are simultaneous deposited on carbon fiber (CF) surface by fiber sizing method. The synergistic effect between GnPs and CNTs in increasing the interfacial and mechanical properties of carbon fiber reinforcement epoxy composites (CFRP) is investigated. The fracture surfaces of the CFRP composites indicated that GnPs/CNTs hybrid coating exhibited the best interfacial and mechanical performance in all coating sample. The interlaminar shear strength of GnPs/CNTs hybrid coated CFRP composites was 90% higher than non-coated CF composites. The flexural and tensile strength of CFRP composites with GnPs/CNTs hybrid coating have an improvement of 52% and 70%, respectively, compared to non-coated CF.
Correction: Falck, R. et al. Microstructure and Mechanical Performance of Additively Manufactured Aluminum 2024-T3/Acrylonitrile Butadiene Styrene Hybrid Joints by AddJoining Technique. Materials 2019, 12, 864
Authors: Falck Rielson , dos Santos Jorge F. , Amancio-Filho Sergio T.
Published: March 2020

The authors wish to make the following correction to the paper [...]