bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Oilseed Rape Seed Treatment with Imidacloprid on Survival, Feeding Behavior, and Detoxifying Enzymes of Mustard Aphid, Lipaphis erysimi
Authors: Huang Fang , Hao Zhongping , Yan Fengming
Published: May 2019

Imidacloprid application, as a seed coating agent on oilseed rape, is recommended to control mustard aphid, Lipaphis erysimi (Kaltenbach) (Hemiptera: Aphididae). In this study, responses of L. erysimi were investigated, including survival, feeding behavior, and detoxifying enzymes, on the oilseed rape seedlings grown from seeds coated with imidacloprid at rates of 6, 12, or 18 g active ingredient (a.i.)/kg seed. The results showed that the aphids’ survival rate, together with that of the progeny of the survivors, on the seed-treated seedlings significantly decreased. This indicates that the aphid population in fields can be suppressed effectively. The electrical penetration graph (EPG) technique was used to record aphid feeding behaviors on two-, four-, and six-leaf stages of oilseed rape seedlings that had been seed-coated with imidacloprid, and individual responses were revealed during the aphid feeding behavior. On the plants at the two-leaf stage, aphid feeding behaviors were influenced, showing decreased frequency of stylet penetration into the leaf (probe) or into the mesophyll cells (potential drops, pds for short), and shortened duration of stylet event in the leaf (probe) or in the phloem. On the plants at the four- and six-leaf stages, these impacts of imidacloprid were weakened; however, the saliva secretion duration in phloem was shortened to less than 5 min in all imidacloprid treatments. The activity of mixed-function oxidase in aphids maintained on the treated seedlings with imidacloprid was elevated. In conclusion, imidacloprid could be used as a seed coating agent for aphid control, but chemical resistance in aphids should not be ignored.
Prognostic Impact of Low Skeletal Muscle Mass on Major Adverse Cardiovascular Events in Coronary Artery Disease: A Propensity Score-Matched Analysis of a Single Center All-Comer Cohort
Authors: Kang Dong Oh , Park So Yeon , Choi Byoung Geol , Na Jin Oh , Choi Cheol Ung , Kim Eung Ju , Rha Seung-Woon , Park Chang Gyu , Hong Suk-Joo , Seo Hong Seog
Published: May 2019

The impact of sarcopenia on atherosclerotic cardiovascular disease remains unclear. We aimed to investigate the prognostic impact of sarcopenia on coronary artery disease (CAD). A total of 475 patients with CAD who underwent successful percutaneous coronary intervention (PCI) and computed tomography (CT) were enrolled. The cross-sectional area of skeletal muscle at the first lumbar (L1) vertebral level was measured, and sex-specific cut-off values of L1 skeletal muscle index (L1 SMI; male <31.00 cm2/m2, female <25.00 cm2/m2) were obtained. The primary outcome was 3-year all-cause mortality and the secondary outcome was 3-year major adverse cardiovascular events (MACEs). Low L1 SMI was present in 141 (29.7%) of 475 patients. The incidence of all-cause mortality (23.7% vs. 5.9%, p < 0.001) and MACEs (39.6% vs. 11.8%, p < 0.001) was significantly higher in patients with low L1 SMI than in those with high L1 SMI. In multivariate analysis, low L1 SMI was an independent predictor of higher risk of all-cause mortality (hazard ratio (HR): 4.07; 95% confidence interval (CI): 1.95–8.45; p < 0.001) and MACEs (HR: 3.76; 95% CI: 2.27–6.23; p < 0.001). These findings remained consistent after propensity score-matched analysis with 91 patient pairs (C-statistic = 0.848). CT-diagnosed low skeletal muscle mass is a powerful predictor of adverse outcomes in patients with CAD undergoing PCI.
Quantitative Analysis of an Intraoperative Digitalized Esophageal Heart Sound Signal to Speculate on Perturbed Cardiovascular Function
Authors: Moon Young-Jin , Kim Sung-Hoon , Park Yong-Seok , Kim Jae-Man , Hwang Gyu-Sam
Published: May 2019

Although visualization of heart sounds, known as phonocardiography, provides valuable information on cardiovascular hemodynamics, its use has not been widely encouraged due to the scarcity of information on its interpretation. In the present study, using the intraoperative phonocardiogram recorded by an esophageal stethoscope, we quantitatively evaluated the time and frequency domains of modulation of the heart sounds components and their association with left ventricular contractility and systemic vascular resistance under the effects of various cardiovascular drugs. We analyzed 29 pairs of intraoperative digitalized phonocardiographic signals and their corresponding hemodynamic data before and after cardiovascular drug administration (ephedrine, esmolol, phenylephrine, and/or nicardipine) in 17 patients who underwent liver transplantation. The S1 and S2 components of the heart sounds (the first and second heart sounds, respectively) were identified and their modulation in time and frequency domains was analyzed. As an index of cardiovascular function, systolic tissue Doppler wave velocity (TDI S’), maximal dP/dt from the arterial waveform, and systemic vascular resistance were simultaneously evaluated. Ephedrine/esmolol and phenylephrine/nicardipine primarily affected the S1 and S2 components of the heart sounds, respectively. This result implies that the intraoperative phonocardiogram may have the potential to be useful in detecting the changes in contractility and afterload that commonly occur in patients receiving anesthesia. In an era of constant need for noninvasive hemodynamic assessment, phonocardiography has the potential for use as a novel and informative tool for monitoring of hemodynamic function.
Influence Mechanism of Different Flow Patterns on the Softening of Red-Bed Soft Rock
Authors: Liu Zhen , He Xinfu , Zhou Cuiying
Published: May 2019

As a typical representative of red beds, the softening and disintegration of red sandstone when it encounters water is an important cause of initiated engineering disasters. However, research on the softening of this kind of rock has mainly focused on the still water–rock interaction. There is still a lack of quantitative analysis and a mechanistic explanation for the basic experimental study of dynamic water–rock interactions. Therefore, based on the independently developed multifunctional open channel hydraulic test equipment, the still water was used as the reference by designing the saturation test of red sandstone under two typical flow patterns—laminar flow and turbulent flow—and combined with a three-dimensional numerical simulation; specifically, the chemical, physical and mechanical effects of different flow patterns on the softening of red sandstone are discussed, and the mechanism of the influence of different flow patterns on the softening of red sandstone was further revealed. The results show that under different flow patterns, as the flow of water increased, the alkalinity of the circulating solution became stronger, the speed of stabilization of the ion concentration became faster, the development of the microscopic structure of the corresponding rock became higher and the decrease in mechanical strength became greater. The flow state affects the processes of rock softening and breaking by acting on the rock from the three aspects of chemistry, physics and mechanics. The study makes up for the deficiency of the quantitative analysis index of rock softening under dynamic water conditions and further improves the influence mechanism of different flow patterns on soft rock softening in red beds under dynamic water conditions. This research also provides a specific method for the protection of estuarine and coastal bank slopes with rich red-bed soft rock dissection under different flow patterns.
Estimation of Coastal Currents Using a Soft Computing Method: A Case Study in Galway Bay, Ireland
Authors: Ren Lei , Miao Jianming , Li Yulong , Luo Xiangxin , Li Junxue , Hartnett Michael
Published: May 2019

In order to obtain forward states of coastal currents, numerical models are a commonly used approach. However, the accurate definition of initial conditions, boundary conditions and other model parameters are challenging. In this paper, a novel application of a soft computing approach, random forests (RF), was adopted to estimate surface currents for three analysis points in Galway Bay, Ireland. Outputs from a numerical model and observations from a high frequency radar system were used as inputs to develop soft computing models. The input variable structure of soft computing models was examined in detail through sensitivity experiments. High correlation of surface currents between predictions from RF models and radar data indicated that the RF algorithm is a most promising means of generating satisfactory surface currents over a long prediction period. Furthermore, training dataset lengths were examined to investigate influences on prediction accuracy. The largest improvement for zonal and meridional surface velocity components over a 59-h forecasting period was 14% and 37% of root mean square error (RMSE) values separately. Results indicate that the combination of RF models with a numerical model can significantly improve forecasting accuracy for surface currents, especially for the meridional surface velocity component.
Mathematical Analysis of a Prototypical Autocatalytic Reaction Network
Authors: Skorb Ekaterina V. , Semenov Sergey N.
Published: May 2019

Network autocatalysis, which is autocatalysis whereby a catalyst is not directly produced in a catalytic cycle, is likely to be more common in chemistry than direct autocatalysis is. Nevertheless, the kinetics of autocatalytic networks often does not exactly follow simple quadratic or cubic rate laws and largely depends on the structure of the network. In this article, we analyzed one of the simplest and most chemically plausible autocatalytic networks where a catalytic cycle is coupled to an ancillary reaction that produces the catalyst. We analytically analyzed deviations in the kinetics of this network from its exponential growth and numerically studied the competition between two networks for common substrates. Our results showed that when quasi-steady-state approximation is applicable for at least one of the components, the deviation from the exponential growth is small. Numerical simulations showed that competition between networks results in the mutual exclusion of autocatalysts; however, the presence of a substantial noncatalytic conversion of substrates will create broad regions where autocatalysts can coexist. Thus, we should avoid the accumulation of intermediates and the noncatalytic conversion of the substrate when designing experimental systems that need autocatalysis as a source of positive feedback or as a source of evolutionary pressure.
Exploiting Plasma Exposed, Natural Surface Nanostructures in Ramie Fibers for Polymer Composite Applications
Authors: Hamad Sameer F. , Stehling Nicola , Hayes Simon A. , Foreman Joel P. , Rodenburg C.
Published: May 2019

Nanoscale surface morphology of plant fibers has important implications for the interfacial bonding in fiber-polymer composites. In this study, we investigated and quantified the effect of plasma-surface modification on ramie plant fibers as a potential tool for simple and efficient surface modification. The extensive investigation of the effects of plasma treatment of the fiber surface nano-morphology and its effect on the fiber-polymer interface was performed by Low-Voltages Scanning Electron Microscopy (LV-SEM), infrared spectroscopy (FT-IR) analysis, fiber-resin angle measurements and mechanical (tensile) testing. The LV-SEM imaging of uncoated plasma treated fibers reveals nanostructures such as microfibrils and elementary fibrils and their importance for fiber mechanical properties, fiber wettability, and fiber-polymer matrix interlocking which all peak at short plasma treatment times. Thus, such treatment can be an effective in modifying the fiber surface characteristics and fiber-polymer matrix interlocking favorably for composite applications.
Effect of Early Age-Curing Methods on Drying Shrinkage of Alkali-Activated Slag Concrete
Authors: Cai Yuxin , Yu Linwen , Yang Yong , Gao Yang , Yang Changhui
Published: May 2019

Drying shrinkage of alkali-activated slag concrete (AASC) is significantly greater than that of concrete made with ordinary Portland cement (OPC). It limits the large-scale application of AASC in field engineering. This study investigates the effect of early age-curing methods, including water curing, curing in elevated-temperature water, and CO2 curing, on drying shrinkage of AASC. Scanning electron microscopy (SEM), X-ray diffraction (XRD), thermogravimetric (TG-DTG), and mercury intrusion porosimetry (MIP) were carried out to analyze the composition and microstructure of hydration products, to provide deeper understanding of drying shrinkage of AASC. The results show that water curing decreased drying shrinkage of both C30 and C50 AASC moderately compared to air curing, while it was more effective for C30 AASC. Curing in water of elevated temperature and CO2 curing were very beneficial to mitigate drying shrinkage of AASC. Heat curing decreased drying shrinkage of AASC up to 80%. SEM and TG-DTG results show that denser microstructure formed because of the accelerated hydration, resulting in lower porosity and lower proportion of pores smaller than 25 nm that contributed to the reduction of drying shrinkage. In addition, under high-temperature curing, most autogenous shrinkage of AASC occurred in the first few days because hydration was accelerated. After measurement of drying shrinkage was started, recorded autogenous shrinkage of AASC cured in elevated-temperature water should be much less than that of AASC cured at normal temperature. It is another important reason for the reduction of drying shrinkage. Carbonation occurring in the CO2 curing period led to the decalcification of C-(A)-S-H gel; it coarsened the pore-size distribution and decreased the total porosity. Therefore, drying shrinkage of C30 and C50 AASC was declined by 49% and 53% respectively.
Tuning the Hydrophobicity of a Hydrogel Using Self-Assembled Domains of Polymer Cross-Linkers
Authors: Kim Hee-Jin , Cho Sungwoo , Oh Seung Joo , Shin Sung Gyu , Ryu Hee Wook , Jeong Jae Hyun
Published: May 2019

Hydrogels incorporated with hydrophobic motifs have received considerable attention to recapitulate the cellular microenvironments, specifically for the bio-mineralization of a 3D matrix. Introduction of hydrophobic molecules into a hydrogel often results in irregular arrangement of the motifs, and further phase separation of hydrophobic domains, but limited efforts have been made to resolve this challenge in developing the hydrophobically-modified hydrogel. Therefore, this study presents an advanced integrative strategy to incorporate hydrophobic domains regularly in a hydrogel using self-assembled domains formed with polymer cross-linkers, building blocks of a hydrogel. Self-assemblies formed by polymer cross-linkers were examined as micro-domains to incorporate hydrophobic motifs in a hydrogel. The self-assembled structures in a pre-gelled solution were confirmed with the fluorescence analysis and the hydrophobicity of a hydrogel could be tuned by incorporating the hydrophobic chains in a controlled manner. Overall, the results of this study would greatly serve to tuning performance of a wide array of hydrophobically-modified hydrogels in drug delivery, cell therapies and tissue engineering.
Effect of Simplified Bonding on Shear Bond Strength between Ceramic Brackets and Dental Zirconia
Authors: Ju Ga-Youn , Oh Soram , Lim Bum-Soon , Lee Hyun-Seung , Chung Shin Hye
Published: May 2019

The aim of this study was to evaluate the long term stability of shear bond strength (SBS) when 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP) containing universal adhesive was used in the ceramic bracket bonding on dental zirconia. Twenty human maxillary incisors were collected. The ceramic bracket was bonded on the buccal enamel surface after the acid-etching and orthodontic primer application (Group CON). Sixty zirconia specimens were sintered, sandblasted and divided into three experimental groups; group CP—ceramic primer followed by an orthodontic primer; group U—universal adhesive; group CU—ceramic primer followed by a universal adhesive. For each specimen, the bracket was bonded onto the treated surface with composite resin (Transbond XT, 3M ESPE). The SBS tested before (CON0, CP0, U0, CU0) and after the artificial aging (CON1, CP1, U1, CU1). The data were statistically analyzed with the Kruskal–Wallis test at a significance level of 0.05. The mean SBS of CON0, CP0, U0 and CU0 were within the clinically acceptable range without significant differences. After the aging process, SBS decreased in all groups. Among the aged groups, CP1 showed the highest SBS. Based on the results, when bonding ceramic brackets to a dental zirconia surface, we can conclude that ceramic primer used with an orthodontic primer, rather than using a universal adhesive, is recommended.
A Single-Stage Manufacturing Model with Imperfect Items, Inspections, Rework, and Planned Backorders
Authors: Wook Kang Chang , Ullah Misbah , Sarkar Mitali , Omair Muhammad , Sarkar Biswajit
Published: May 2019

Each industry prefers to sell perfect products in order to maintain its brand image. However, due to a long-run single-stage production system, the industry generally obtains obstacles. To solve this issue, a single-stage manufacturing model is formulated to make a perfect production system without defective items. For this, the industry decides to stop selling any products until whole products are ready to fulfill the order quantity. Furthermore, manufacturing managers prefer product qualification from the inspection station especially when processes are imperfect. The purpose of the proposed manufacturing model considers that the customer demands are not fulfilled during the production phase due to imperfection in the process, however customers are satisfied either at the end of the inspection process or after reworking the imperfect products. Rework operation, inspection process, and planned backordering are incorporated in the proposed model. An analytical approach is utilized to optimize the lot size and planned backorder quantities based on the minimum average cost. Numerical examples are used to illustrate and compare the proposed model with previously developed models. The proposed model is considered more beneficial in comparison with the existing models as it incorporates imperfection, rework, inspection rate, and planned backorders.
Preliminary Analysis of a Fully Ceramic Microencapsulated Fuel Thermal–Mechanical Performance
Authors: Chen Ping , Qiu Suizheng , Liu Shichao , Zhou Yi , Xin Yong , Gao Shixin , Qiu Xi , Lu Huaiyu
Published: May 2019

In this paper, a two-dimensional characteristic unit was used to simulate the thermal–mechanical performance of a fully ceramic microencapsulated (FCM) fuel pellet, and the criterion of FCM structure integrity was discussed. FCM structure integrity can be reflected though the integrity of the silicon carbide (SiC) matrix or SiC layers because of the excellent fission retention capability of SiC ceramics. The maximum temperature of the SiC matrix under normal conditions of the pressure water reactor (PWR) environment was about 1390 K, which was lower than the decomposition point of SiC. The maximum hoop stress of the SiC matrix, especially the inner part, was up to about 1200 MPa, and the hoop stress of the non-fuel region part was lower than the inner part, which can be attributed to the deformation of tristructural-isotopic (TRISO) particles. The hoop stress of the SiC layers at the end of life was only about 180 MPa, which is much lower than the strength of the chemical vapor deposition (CVD)-SiC. The failure probability of the SiC layer was lower than 9 × 10−5; thus, the integrity of SiC layers and the fission retention capability were maintained. The structure integrity of FCM fuel was broken because the SiC matrix cracked.
A Testing Coverage Model Based on NHPP Software Reliability Considering the Software Operating Environment and the Sensitivity Analysis
Authors: Song Kwang Yoon , Chang In Hong , Pham Hoang
Published: May 2019

We have been attempting to evaluate software quality and improve its reliability. Therefore, research on a software reliability model was part of the effort. Currently, software is used in various fields and environments; hence, one must provide quantitative confidence standards when using software. Therefore, we consider the testing coverage and uncertainty or randomness of an operating environment. In this paper, we propose a new testing coverage model based on NHPP software reliability with the uncertainty of operating environments, and we provide a sensitivity analysis to study the impact of each parameter of the proposed model. We examine the goodness-of-fit of a new testing coverage model based on NHPP software reliability and other existing models based on two datasets. The comparative results for the goodness-of-fit show that the proposed model does significantly better than the existing models. In addition, the results for the sensitivity analysis show that the parameters of the proposed model affect the mean value function.
Influence of Austenite Grain Size on Mechanical Properties after Quench and Partitioning Treatment of a 42SiCr Steel
Authors: Härtel Sebastian , Awiszus Birgit , Graf Marcel , Nitsche Alexander , Böhme Marcus , Wagner Martin F.-X. , Jirkova Hana , Masek Bohuslav
Published: May 2019

This paper examines how the initial austenite grain size in quench and partitioning (Q-P) processes influences the final mechanical properties of Q-P steels. Differences in austenite grain size distribution may result, for example, from uneven heating rates of semi-finished products prior to a forging process. In order to quantify this influence, a carefully defined heat treatment of a cylindrical specimen made of the Q-P-capable 42SiCr steel was performed in a dilatometer. Different austenite grain sizes were adjusted by a pre-treatment before the actual Q-P process. The resulting mechanical properties were determined using the upsetting test and the corresponding microstructures were analyzed by scanning electron microscopy (SEM). These investigations show that a larger austenite grain size prior to Q-P processing leads to a slightly lower strength as well as to a coarser martensitic microstructure in the Q-P-treated material.
All You Need to Know about the Kinetics of Thermally Stimulated Reactions Occurring on Cooling
Authors: Liavitskaya Tatsiana , Vyazovkin Sergey
Published: May 2019

In this tutorial overview article the authors share their original experience in studying the kinetics of thermally stimulated reactions under the conditions of continuous cooling. It is stressed that the kinetics measured on heating is similar to that measured on cooling only for single-step reactions. For multi-step reactions the respective kinetics can differ dramatically. The application of an isoconversional method to thermogravimetry (TGA) or differential scanning calorimetry (DSC) data allows one to recognize multi-step kinetics in the form of the activation energy that varies with conversion. Authors’ argument is supported by theoretical considerations as well as by experimental examples that include the reactions of thermal decomposition and crosslinking polymerization (curing). The observed differences in the kinetics measured on heating and cooling ultimately manifest themselves in the Arrhenius plots of the opposite curvatures, which means that the heating kinetics cannot be used to predict the kinetics on cooling. The article provides important background knowledge necessary for conducting successful kinetic studies on cooling. It includes a practical advice on optimizing the parameters of cooling experiments as well as on proper usage of kinetic methods for analysis of obtained data.
Feasibility of Utilizing Stable-Isotope Dimethyl Labeling in Liquid Chromatography–Tandem Mass Spectrometry-Based Determination for Food Allergens—Case of Kiwifruit
Authors: Shih Yi-Chen , Hsiao Jhih-Ting , Sheu Fuu
Published: May 2019

Stable-isotope dimethyl labeling is a highly reactive and cost-effective derivatization procedure that could be utilized in proteomics analysis. In this study, a liquid chromatography– tandem mass spectrometry in multiple reaction monitoring mode (LC-MS-MRM) platform for the quantification of kiwi allergens was first developed using this strategy. Three signature peptides for target allergens Act d 1, Act d 5, and Act d 11 were determined and were derivatized with normal and deuterated formaldehyde as external calibrants and internal standards, respectively. The results showed that sample preparation with the phenol method provided comprehensive protein populations. Recoveries at four different levels ranging from 72.5–109.3% were achieved for the H-labeled signature peptides of Act d 1 (SPA1-H) and Act d 5 (SPA5-H) with precision ranging from 1.86–9.92%. The limit of quantification (LOQ) was set at 8 pg mL−1 for SPA1-H and at 8 ng mL−1 for SPA5-H. The developed procedure was utilized to analyze seven kinds of hand-made kiwi foods containing 0.0175–0.0515 mg g−1 of Act d 1 and 0.0252–0.0556 mg g−1 of Act d 5. This study extended the applicability of stable-isotope dimethyl labeling to the economical and precise determination of food allergens and peptides.
Cylindrical Layered Bone Scaffolds with Anisotropic Mechanical Properties as Potential Drug Delivery Systems
Authors: Di Filippo Maria Francesca , Amadori Sofia , Casolari Sonia , Bigi Adriana , Dolci Luisa Stella , Panzavolta Silvia
Published: May 2019

3D cylindrical layered scaffolds with anisotropic mechanical properties were prepared according to a new and simple method, which involves gelatin foaming, deposition of foamed strips, in situ crosslinking, strip rolling and lyophilization. Different genipin concentrations were tested in order to obtain strips with different crosslinking degrees and a tunable stability in biological environment. Before lyophilization, the strips were curled in a concentric structure to generate anisotropic spiral-cylindrical scaffolds. The scaffolds displayed significantly higher values of stress at break and of the Young modulus in compression along the longitudinal than the transverse direction. Further improvement of the mechanical properties was achieved by adding strontium-substituted hydroxyapatite (Sr-HA) to the scaffold composition and by increasing genipin concentration. Moreover, composition modulated also water uptake ability and degradation behavior. The scaffolds showed a sustained strontium release, suggesting possible applications for the local treatment of abnormally high bone resorption. This study demonstrates that assembly of layers of different composition can be used as a tool to obtain scaffolds with modulated properties, which can be loaded with drugs or biologically active molecules providing properties tailored upon the needs.
Inhibitory Mechanisms of DHA/CQ on pH and Iron Homeostasis of Erythrocytic Stage Growth of Plasmodium falciparum
Authors: Tang Tian , Xu Wenhui , Ma Ji , Wang Huajing , Cui Zhao , Jiang Tingliang , Li Canghai
Published: May 2019

Malaria is an infectious disease caused by Plasmodium group. The mechanisms of antimalarial drugs DHA/CQ are still unclear today. The inhibitory effects (IC50) of single treatments with DHA/CQ or V-ATPase inhibitor Baf-A1 or combination treatments by DHA/CQ combined with Baf-A1 on the growth of Plasmodium falciparum strain 3D7 was investigated. Intracellular cytoplasmic pH and labile iron pool (LIP) were labeled by pH probe BCECF, AM and iron probe calcein, AM, the fluorescence of the probes was measured by FCM. The effects of low doses of DHA (0.2 nM, 0.4 nM, 0.8 nM) on gene expression of V-ATPases (vapE, vapA, vapG) located in the membrane of DV were tested by RT-qPCR. DHA combined with Baf-A1 showed a synergism effect (CI = 0.524) on the parasite growth in the concentration of IC50. Intracellular pH and irons were effected significantly by different doses of DHA/Baf-A1. Intracellular pH was decreased by CQ combined with Baf-A1 in the concentration of IC50. Intracellular LIP was increased by DHA combined with Baf-A1 in the concentration of 20 IC50. The expression of gene vapA was down-regulated by all low doses of DHA (0.2/0.4/0.8 nM) significantly (p < 0.001) and the expression of vapG/vapE were up-regulated by 0.8 nM DHA significantly (p < 0.001). Interacting with ferrous irons, affecting the DV membrane proton pumping and acidic pH or cytoplasmic irons homeostasis may be the antimalarial mechanism of DHA while CQ showed an effect on cytoplasmic pH of parasite in vitro. Lastly, this article provides us preliminary results and a new idea for antimalarial drugs combination and new potential antimalarial combination therapies.
Ganoderma lucidum Extract Reduces the Motility of Breast Cancer Cells Mediated by the RAC–Lamellipodin Axis
Authors: Acevedo-Díaz Ariana , Ortiz-Soto Gabriela , Suárez-Arroyo Ivette J. , Zayas-Santiago Astrid , Martínez Montemayor Michelle M.
Published: May 2019

Breast cancer (BC) is the second leading cause of cancer death among women worldwide. The main cause of BC morbidity and mortality is the invasiveness capacity of cancer cells that may lead to metastasis. Here, we aimed to investigate the therapeutic efficacy of Ganoderma lucidum extract (GLE)—a medicinal mushroom with anticancer properties—on BC motility via the Rac/Lamellipodin pathway. GLE treatment effects were tested on MDA-MB-231 breast cancer cells. The effects were tested on cell viability, migration and invasion. Pulldowns, immunoblotting, and immunofluorescence were used to measure Rac activity and the expression of proteins involved in cell migration and in lamellipodia formation, respectively. As a result, GLE suppressed BC cell viability, migration, and invasion capacity. GLE impaired Rac activity, as well as downregulated Lamellipodin, ENA/VASP, p-FAK (Tyr925), Cdc42, and c-Myc expression. Lamellipodia formation was significantly reduced by GLE. In conclusion, we demonstrate that GLE reduces Rac activity and downregulates signaling molecules involved in lamellipodia formation. These novel findings serve as basis for further studies to elucidate the potential of GLE as a therapeutic agent regulating the Rac/Lamellipodin pathway in BC metastasis.
Flexible Organic Thermoelectric Materials and Devices for Wearable Green Energy Harvesting
Authors: Zhang Yinhang , Park Soo-Jin
Published: May 2019

In the past few decades, organic thermoelectric materials/devices, which can exhibit remarkable potential in green energy conversion, have drawn great attention and interest due to their easy processing, light weight, intrinsically low thermal conductivity, and mechanical flexibility. Compared to traditional batteries, thermoelectric materials have high prospects as alternative power generators for harvesting green energy. Although crystalline inorganic semiconductors have dominated the fields of thermoelectric materials up to now, their practical applications are limited by their intrinsic fragility and high toxicity. The integration of organic polymers with inorganic nanoparticles has been widely employed to tailor the thermoelectric performance of polymers, which not only can combine the advantages of both components but also display interesting transport phenomena between organic polymers and inorganic nanoparticles. In this review, parameters affecting the thermoelectric properties of materials were briefly introduced. Some recently developed n-type and p-type thermoelectric films and related devices were illustrated along with their thermoelectric performance, methods of preparation, and future applications. This review will help beginners to quickly understand and master basic knowledge of thermoelectric materials, thus inspiring them to design and develop more efficient thermoelectric devices.
Distinct and Quantitative Validation Method for Predictive Process Modeling with Examples of Liquid-Liquid Extraction Processes of Complex Feed Mixtures
Authors: Schmidt Axel , Strube Jochen
Published: May 2019

As of today, industrial process development for liquid-liquid extraction and scale-up of extraction columns is based on an experimental procedure that requires tests in pilot-scale. This methodology consumes large amounts of material and time and the utilized scale-up equations are crude estimates including considerable safety margins. This approach is practical for well-known systems or low-value products coupled with high production scale, where such a scale-up methodology has less impact on the overall profitability. However, for new high-value products in biologics manufacturing, a process development based on process understanding and the use of validated process models is imperative. Therefore, a distinct and quantitative validation workflow for liquid-liquid extraction modeling is presented on the example of two complex feed mixtures. Monte-Carlo simulations based on the presented model parameter determination concept result for both examples in prediction accuracy comparable to the experiments and prediction precision within the deviation of the respective experiments. Identification of statistically significant parameters is demonstrated. The presented methodology for model validation will support the implementation of liquid-liquid extraction in the manufacturing of new high value biological products in regulated industries by providing a workflow to derive a Quality-by-Design compatible process model.
Revisiting Calibration of the Solvency II Standard Formula for Mortality Risk: Does the Standard Stress Scenario Provide an Adequate Approximation of Value-at-Risk?
Authors: Gylys Rokas , Šiaulys Jonas
Published: May 2019

The primary objective of this work is to analyze model based Value-at-Risk associated with mortality risk arising from issued term life assurance contracts and to compare the results with the capital requirements for mortality risk as determined using Solvency II Standard Formula. In particular, two approaches to calculate Value-at-Risk are analyzed: one-year VaR and run-off VaR. The calculations of Value-at-Risk are performed using stochastic mortality rates which are calibrated using the Lee-Carter model fitted using mortality data of selected European countries. Results indicate that, depending on the approach taken to calculate Value-at-Risk, the key factors driving its relative size are: sensitivity of technical provisions to the latest mortality experience, volatility of mortality rates in a country, policy term and benefit formula. Overall, we found that Solvency II Standard Formula on average delivers an adequate capital requirement, however, we also highlight particular situations where it could understate or overstate portfolio specific model based Value-at-Risk for mortality risk.
A Semi-Supervised Monocular Stereo Matching Method
Authors: Zhang Zhimin , Qiao Jianzhong , Lin Shukuan
Published: May 2019

Supervised monocular depth estimation methods based on learning have shown promising results compared with the traditional methods. However, these methods require a large number of high-quality corresponding ground truth depth data as supervision labels. Due to the limitation of acquisition equipment, it is expensive and impractical to record ground truth depth for different scenes. Compared to supervised methods, the self-supervised monocular depth estimation method without using ground truth depth is a promising research direction, but self-supervised depth estimation from a single image is geometrically ambiguous and suboptimal. In this paper, we propose a novel semi-supervised monocular stereo matching method based on existing approaches to improve the accuracy of depth estimation. This idea is inspired by the experimental results of the paper that the depth estimation accuracy of a stereo pair as input is better than that of a monocular view as input in the same self-supervised network model. Therefore, we decompose the monocular depth estimation problem into two sub-problems, a right view synthesized process followed by a semi-supervised stereo matching process. In order to improve the accuracy of the synthetic right view, we innovate beyond the existing view synthesis method Deep3D by adding a left-right consistency constraint and a smoothness constraint. To reduce the error caused by the reconstructed right view, we propose a semi-supervised stereo matching model that makes use of disparity maps generated by a self-supervised stereo matching model as the supervision cues and joint self-supervised cues to optimize the stereo matching network. In the test, the two networks are able to predict the depth map directly from a single image by pipeline connecting. Both procedures not only obey geometric principles, but also improve estimation accuracy. Test results on the KITTI dataset show that this method is superior to the current mainstream monocular self-supervised depth estimation methods under the same condition.
Development of an Anti-Idiotypic VHH Antibody and Toxin-Free Enzyme Immunoassay for Ochratoxin A in Cereals
Authors: Zhang Caixia , Zhang Qi , Tang Xiaoqian , Zhang Wen , Li Peiwu
Published: May 2019

Enzyme-linked immunosorbent assay (ELISA) test kits have been widely used for the determination of mycotoxins in agricultural products and foods, however, this test uses toxin standards with high toxicity and carcinogenicity that seriously threaten human health. In this work, the anti-idiotypic nanobody VHH 2-24 was first developed and then, using it as a surrogate standard, a toxin-free enzyme immunoassay for ochratoxin A (OTA) was established. The IC50 value of the VHH 2-24 surrogate standard-based ELISA was 0.097 µg/mL, with a linear range of 0.027–0.653 µg/mL. The average recoveries were tested by spike-and-recovery experiments, and ranged from 81.8% to 105.0%. The accuracy of the developed ELISA for detecting OTA was further verified by using the high performance liquid chromatography (HPLC) method, and an excellent correlation was observed. In summary, the toxin-free ELISA established in this study proves the latent use of the anti-idiotypic VHH as a surrogate calibrator for other mycotoxins and highly toxic small molecule analysis to improve assay properties for highly sensitive analyte determination in agricultural products.
Binding to The Target Cell Surface Is The Crucial Step in Pore Formation of Hemolysin BL from Bacillus cereus
Authors: Jessberger Nadja , Dietrich Richard , Schwemmer Stefanie , Tausch Franziska , Schwenk Valerie , Didier Andrea , Märtlbauer Erwin
Published: May 2019

A major virulence factor involved in Bacillus cereus food poisoning is the three-component enterotoxin hemolysin BL. It consists of the binding component B and the two lytic components L1 and L2. Studying its mode of action has been challenging, as natural culture supernatants additionally contain Nhe, the second three-component enterotoxin, and purification of recombinant (r) Hbl components has been difficult. In this study, we report on pore-forming, cytotoxic, cell binding and hemolytic activity of recently generated rHbl components expressed in E. coli. It is known that all three Hbl components are necessary for cytotoxicity and pore formation. Here we show that an excess of rHbl B enhances, while an excess of rHbl L1 hinders, the velocity of pore formation. Most rapid pore formation was observed with ratios L2:L1:B = 1:1:10 and 10:1:10. It was further verified that Hbl activity is due to sequential binding of the components B - L1 - L2. Accordingly, all bioassays proved that binding of Hbl B to the cell surface is the crucial step for pore formation and cytotoxic activity. Binding of Hbl B took place within minutes, while apposition of the following L1 and L2 occurred immediately. Further on, applying toxin components simultaneously, it seemed that Hbl L1 enhanced binding of B to the target cell surface. Overall, these data contribute significantly to the elucidation of the mode of action of Hbl, and suggest that its mechanism of pore formation differs substantially from that of Nhe, although both enterotoxin complexes are sequentially highly related.
Exploring Public Preferences, Priorities, and Policy Perspectives for Controlling Invasive Mosquito Species in Greece
Authors: Kolimenakis Antonios , Latinopoulos Dionysios , Bithas Kostas , Richardson Clive , Lagouvardos Konstantinos , Stefopoulou Angeliki , Papachristos Dimitrios , Michaelakis Antonios
Published: May 2019

Climate change, urbanization, and financial crisis have created a dramatic mixture of challenges in Southern Europe, increasing further the risks of transmission of new vector-borne diseases. In the last decade, there has been a wide spread of an invasive mosquito species Aedes albopictus, commonly known as the Asian tiger mosquito, in various urban ecosystems of Greece accompanied by greater risks of infectious diseases, higher nuisance levels, and increased expenses incurred for their control. The aim of the present paper is to investigate citizens’ perception of the Aedes albopictus problem and to evaluate various policy aspects related to its control. Findings are based on the conduct of a web-based survey at a national scale and the production of national surveillance maps. Results indicate that citizens are highly concerned with the health risks associated with the new mosquito species and consider public prevention strategies highly important for the confrontation of the problem while, at the same time, surveillance maps indicate a constant intensification of the problem. The spatial patterns of these results are further investigated aiming to define areas (regions) with different: (a) Levels of risk and/or (b) policy priorities. It appears that citizens are aware of the invasive mosquito problem and appear prone to act against possible consequences. Climate change and the complex socio-ecological context of South Europe are expected to favor a deterioration of the problem and an increasing risk of the transmission of new diseases, posing, in this respect, new challenges for policy makers and citizens.
Production and Detection of Light Dark Matter at Jefferson Lab: The BDX Experiment
Authors: De Napoli Marzio
Published: May 2019

The Beam Dump eXperiment (BDX) is a an electron-beam thick-target experiment aimed to investigate the existence of light Dark Matter particles in the MeV-GeV mass region at Jefferson Lab. The experiment will make use of a 10.6 GeV high-intensity electron-beam impinging on the Hall-A beam-dump to produce the Dark Matter particles ( χ ) through the Dark Photon portal. The BDX detector located at ∼20 m from the dump consists of two main components: an electromagnetic calorimeter to detect the signals produced by the χ -electron scattering and a veto system to reject background. The expected signature of the DM (Dark Matter) interaction in the Ecal (Electromagnetic calorimeter) is a ∼GeV electromagnetic shower paired with a null activity in the surrounding active veto counters. Collecting 10 22 electrons on target in 285 days of parasitic run at 65 μ A of beam current, and with an expected background of O(5) counts, in the case of a null discovery, BDX will be able to lower the exclusion limits by one to two orders of magnitude in the parameter space of dark-matter coupling versus mass. This paper describes the experiment and presents a summary of the most significant results achieved thus far, which led to the recent approval of the experiment by JLab-PAC46.
Coping with Floods in Pikine, Senegal: An Exploration of Household Impacts and Prevention Efforts
Authors: Hungerford Hilary , Smiley Sarah , Blair Taylor , Beutler Samantha , Bowers Noel , Cadet Eddy
Published: May 2019

African cities are at increasing risk for disasters, including floods. Pikine, Senegal—located on the outskirts of the Dakar metropolitan region—has experienced regular floods since 2005 due to a rising water table, dense settlement, and inadequate drainage. The goal of this research was to assess household experiences of floods through in-depth qualitative interviews in one area of Pikine. A total of 44 households were interviewed on the economic and health impacts of flooding and their perceptions of flood mitigation strategies. Our research confirmed that floods create substantial economic and health burdens for families and that infrastructure projects have helped, but not solved, the flooding issues. Our research also had some unexpected findings, particularly relating to concerns over drinking water, land tenure and housing prices, and perception of government intervention.
Roles of Hepatitis B Virus Mutations in the Viral Reactivation after Immunosuppression Therapies
Authors: Inoue Jun , Nakamura Takuya , Masamune Atsushi
Published: May 2019

Reactivation of hepatitis B virus (HBV) is a major problem in patients receiving chemotherapy for malignant diseases or immunosuppression therapies. It has been thought that a reduction in the immune responses might result in the reactivation of HBV replication from covalently closed circular DNA (cccDNA) residing in hepatocytes. However, not only the host’s immune status, but also viral mutations have been reported to be associated with reactivation. Especially, several case reports about amino acid mutations in hepatitis B surface antigen (HBsAg) that escape from immune reactions have been reported, and recent reports showed that the frequencies of such mutations are higher than previously expected. In this review, we summarize the characteristics of viral mutations, including immune escape mutations in HBV-reactivated patients, and discuss their significance.
Tropical Andes Radar Precipitation Estimates Need High Temporal and Moderate Spatial Resolution
Authors: Guallpa Mario , Orellana-Alvear Johanna , Bendix Jörg
Published: May 2019

Weather radar networks are an excellent tool for quantitative precipitation estimation (QPE), due to their high resolution in space and time, particularly in remote mountain areas such as the Tropical Andes. Nevertheless, reduction of the temporal and spatial resolution might severely reduce the quality of QPE. Thus, the main objective of this study was to analyze the impact of spatial and temporal resolutions of radar data on the cumulative QPE. For this, data from the world’s highest X-band weather radar (4450 m a.s.l.), located in the Andes of Ecuador (Paute River basin), and from a rain gauge network were used. Different time resolutions (1, 5, 10, 15, 20, 30, and 60 min) and spatial resolutions (0.5, 0.25, and 0.1 km) were evaluated. An optical flow method was validated for 11 rainfall events (with different features) and applied to enhance the temporal resolution of radar data to 1-min intervals. The results show that 1-min temporal resolution images are able to capture rain event features in detail. The radar–rain gauge correlation decreases considerably when the time resolution increases (r from 0.69 to 0.31, time resolution from 1 to 60 min). No significant difference was found in the rain total volume (3%) calculated with the three spatial resolution data. A spatial resolution of 0.5 km on radar imagery is suitable to quantify rainfall in the Andes Mountains. This study improves knowledge on rainfall spatial distribution in the Ecuadorian Andes, and it will be the basis for future hydrometeorological studies.