bluuebunny/crossref_metadata_2025 huggingface dataset:
The VEGF Inhibitor Soluble Fms-like Tyrosine Kinase 1 Does Not Promote AKI-to-CKD Transition
Authors: van Aanhold Cleo C. L. , Koudijs Angela , Dijkstra Kyra L. , Wolterbeek Ron , Bruijn Jan A. , van Kooten Cees , Baelde Hans J.
Published: August 2022

(1) Background: Soluble Fms-like tyrosine kinase 1 (sFLT1) is an endogenous VEGF inhibitor. sFLT1 has been described as an anti-inflammatory treatment for diabetic nephropathy and heart fibrosis. However, sFLT1 has also been related to peritubular capillary (PTC) loss, which promotes fibrogenesis. Here, we studied whether transfection with sFlt1 aggravates experimental AKI-to-CKD transition and whether sFLT1 is increased in human kidney fibrosis. (2) Methods: Mice were transfected via electroporation with sFlt1. After confirming transfection efficacy, mice underwent unilateral ischemia/reperfusion injury (IRI) and were sacrificed 28 days later. Kidney histology and RNA were analyzed to study renal fibrosis, PTC damage and inflammation. Renal sFLT1 mRNA expression was measured in CKD biopsies and control kidney tissue. (3) Results: sFlt1 transfection did not aggravate renal fibrosis, PTC loss or macrophage recruitment in IRI mice. In contrast, higher transfection efficiency was correlated with reduced expression of pro-fibrotic and pro-inflammatory markers. In the human samples, sFLT1 mRNA levels were similar in CKD and control kidneys and were not correlated with interstitial fibrosis or PTC loss. (4) Conclusion: As we previously found that sFLT1 has therapeutic potential in diabetic nephropathy, our findings indicate that sFLT1 can be administered at a dose that is therapeutically effective in reducing inflammation, without promoting maladaptive kidney damage.
Seismic Response of a Two-Story Three-Span Subway Structural Model under High-Accelerated Geotechnical Centrifuge Shaking Table Test
Authors: Zhao Dongdong , Liu Jinbo
Published: August 2022

The seismic response of underground structures such as subway stations is critical. However, the complex underground structure experiments under a dynamic centrifuge shaking table are significantly limited. This study conducts a shaking table test of a two-story three-span underground structure under 50 g gravitational centrifuge acceleration to investigate soil–structure interaction effects (SSI). The test is performed on a sand soil-structural model using a laminar shear box with depths of 2.5 m, and the input motion is a Parkfield wave. The experimental results indicate that the central column of the two-story three-span underground structure is the weak component during the earthquake. In addition, the numerical simulations of the soil–structure system are carried out to study the effect of buried depth and foundation soil type on the seismic response of the underground structures. The experimental and numerical results proved that the performed centrifuge test can reproduce the key seismic response characteristics of the SSI in the prototype underground structure and provided guidelines to design a similar underground structure in the future development of the urban subway systems.
Percutaneous Coronary Angioplasty in Patients with Cancer: Clinical Challenges and Management Strategies
Authors: Doolub Gemina , Mamas Mamas A.
Published: August 2022

The number of cancer survivors in the United States is projected to increase by 31% by 2030. With advances in early screening, diagnosis and therapeutic strategies, a steadily increasing number of patients are surviving cancer. Coronary artery disease (CAD) is now one of the leading causes of death amongst cancer survivors, with the latter group of patients having a higher risk of CAD compared to the general population. Our review covers a range of specific challenges faced by doctors when considering percutaneous coronary interventions (PCI) in cancer patients; clinical outcomes in cancer patients undergoing PCI, as well as some important technical considerations to be made when making decisions regarding the management strategy in this special population of patients.
A Comparative Study of Short-Term Vascular and Stromal Alterations of the Choroid Following Half-Fluence Photodynamic Therapy in Pachychoroid Neovasculopathy and Chronic Central Serous Chorioretinopathy
Authors: Yanık Özge , Demirel Sibel , Batıoğlu Figen , Özmert Emin
Published: August 2022

Background: Central serous chorioretinopathy (CSCR) and pachychoroid neovasculopathy (PNV) are among the pachychoroid spectrum diseases (PSDs). Half-fluence photodynamic therapy (hf-PDT) is one of the effective treatment methods for both diseases. The aim of this study was to compare the effect of hf-PDT on the choroidal structure in CSCR and PNV. Methods: This study included 35 patients with chronic CSCR and 18 patients with PNV. The hf-PDT protocol was applied to all eyes. Before and 3 months after hf-PDT, enhanced-depth optical coherence tomography images were analyzed. The total choroidal area (CA), luminal area (LA), and stromal area (SA) were measured using ImageJ software. Results: Compared with baseline values, 3 months after hf-PDT, the mean CA reduced from 1.398 to 1.197 mm2 (p < 0.001) in the CSCR group and the total CA reduced from 1.050 to 1.000 mm2 (p < 0.021) in the PNV group. The mean percentage changes in CA, LA, and SA values were statistically higher in the chronic CSCR group (13.86%, 13.53%, and 14.11%, respectively) than those in the PNV group (4.61%, 4.02%, and 5.74%; p = 0.001, p = 0.002, and p = 0.031, respectively). Conclusion: CSCR and PNV are thought to be PSDs. However, they differ in choroidal morphological response after hf-PDT, which might be a result of the different structural components of the PNV lesions.
Variously Prepared Zeolite Y as a Modifier of ANFO
Authors: Kuterasiński Łukasz , Wojtkiewicz Agnieszka M. , Sadowska Marta , Żeliszewska Paulina , Napruszewska Bogna D. , Zimowska Małgorzata , Pytlik Mateusz , Biessikirski Andrzej
Published: August 2022

In the presented research, we investigated Ammonium Nitrate Fuel Oil (ANFO), with the addition of variously modified zeolite Y as an attractive explosive. Analysis of both blasting tests and thermodynamic models of blasting properties led to the conclusion that the addition of zeolite Y enhanced the detonation properties of such prepared ANFO via the growth of the detonation pressure, temperature, compression energy, and heat of the explosion. Generally, the modification of ANFO with variously prepared zeolite Y also reduced the volume of (COx + NOx) post-blast fumes. Furthermore, it was found that the ANFO’s velocity of detonation (VOD) could be controlled by the choice of the way of zeolite Y modification. Namely, for zeolite Y without Mg, as well as Mg-Y prepared via the impregnation method, the VOD rose. The opposite effect was observed when ANFO was modified with Mg-Y, obtained from the deposition of Mg over zeolite Y via the ultrasonic-assisted procedure.
Improving the DC Dielectric Properties of XLPE with Appropriate Content of Dicumyl Peroxide for HVDC Cables Insulation
Authors: Ahmed Muneeb , Zhong Lisheng , Li Fei , Xu Nuo , Gao Jinghui
Published: August 2022

In this research, crosslinked polyethylene (XLPE) is developed with selective content of dicumyl peroxide (DCP), and the influence of microstructural properties and chemical composition on the mechanical and direct current (DC) dielectric properties are investigated. The measurements for the microstructural analysis are taken by gel permeation chromatography (GPC), differential scanning calorimetry (DSC), gel content test and Fourier transform infrared (FTIR). The mechanical properties of XLPE are evaluated by hot-set test. The results of microstructural and chemical composition show that the increase in DCP content increases the crosslinking degree from 74.3% to 81.6%, reduces the crystallinity/lamella thickness from 36.8% to 35.5%/7.6–7.1 nm, reduces the average molecular weight between two crosslinks by 0.01 kg/mol and reduces the oxidation level/carbonyl index. The increase in DCP in XLPE samples decreases the permanent elongation from 2.2% to 0% and elongation rate from 300% to 80% of the cable insulation. The rise in DCP content increases the crosslinking degree due to which the DC resistivity and activation energy is increased. The DC breakdown strength at 30–90 °C is increased due to the increase in crosslinking degree and reduction in carbonyl index/oxidation level. The space charge accumulation is measured at 30 °C under 20–60 kV/mm, resulting in less homo-charges and hetero-charges with the increase in DCP. It is proven that the role of appropriate DCP content is vital in increasing the DC dielectric performance, internal material characteristics and mechanical performance of XLPE.
Experiment and Validation of Local Bearing Capacity for Ultra-High-Performance Concrete Confined with Stirrups
Authors: Musa Moneef Mohamed Elobaid , Xiong Xueyu , Zhang Yang
Published: August 2022

Ultra-high-performance concrete (UHPC) has the advantages of high compressive and tensile strength, high bending strength, good durability, remarkable corrosion resistance, and low self-weight. In this study, ten UHPC specimens were designed based on three fundamental parameters, including the ratio of the gross supporting area Ab to the bearing plate area Al (local area aspect ratio Ab/Al), the ratio of core area Acor to the bearing plate area Al (core area aspect ratio Acor/Al,), and the reinforcement ratio pv, to investigate mechanical behaviors and bearing capacity. Failure modes, cracking load, crack propagation, wedge features, the relationship between local compression and deformation, and the local bearing capacity was investigated. Finite element models (FEMs) were built to simulate and validate the observed behavior of the anchorage zone under compressive loading. The experiment results demonstrate that the pv significantly increases the bearing capacity. When the reinforcement ratio increased from 4.5% to 3.7%, the bearing capacity increased by 23%, and the effect of Acor/Al was not obvious. In addition, decreasing the Ab/Al from 11.1 to 6.3 increases the bearing capacity to 19%. Furthermore, a model was proposed to predict the bearing capacity of the UHPC specimens reinforced with stirrups. The calculated values, numerical predictions, and experiment results showed good agreement.
Effect of B on Microstructure and Properties of Surfacing Layer of Austenitic Stainless Steel Flux Cored Wire
Authors: Guo Jianbo , Liu Zhengjun , Su Yunhai
Published: August 2022

In order to study the effect of element B on the corrosion resistance of stainless steel-based flux cored wire surfacing alloy, a stainless steel surfacing layer was prepared on the surface of carbon steel plate by melt electrode gas shielded welding, and then the microstructure, electrochemical corrosion resistance, and wear resistance of the surfacing layer were analyzed. The results show that the surfacing layer of surfacing alloy presents M2B and Fe3(C, B) phases based on austenite. Boride formed in deposited metal has good corrosion resistance. Therefore, adding the proper amount of B can significantly improve the corrosion resistance of the surfacing layer. When the boron content is 2%, the corrosion resistance is the best. The minimum self-corrosion current density is 1.75766 × 10−11 mA·cm2, and the maximum self-corrosion potential is −0.254438 V. Maximum impedance curve radius. At this time, the wear resistance of the surfacing layer is also the best.
A Multiscale Overview of Modelling Rolling Cyclic Fatigue in Bearing Elements
Authors: Abdullah Muhammad U. , Khan Zulfiqar A.
Published: August 2022

During service, bearing components experience rolling cyclic fatigue (RCF), resulting in subsurface plasticity and decay of the parent microstructure. The accumulation of micro strains spans billions of rolling cycles, resulting in the continuous evolution of the bearing steel microstructure. The bearing steel composition, non-metallic inclusions, continuously evolving residual stresses, and substantial work hardening, followed by subsurface softening, create further complications in modelling bearing steel at different length scales. The current study presents a multiscale overview of modelling RCF in terms of plastic deformation and the corresponding microstructural alterations. This article investigates previous models to predict microstructural alterations and material hardening approaches widely adopted to mimic the cyclic hardening response of the evolved bearing steel microstructure. This review presents state-of-the-art, relevant reviews in terms of this subject and provides a robust academic critique to enhance the understanding of the elastoplastic response of bearing steel under non-proportional loadings, damage evolution, and the formation mechanics of microstructural alterations, leading to the increased fatigue life of bearing components. It is suggested that a multidisciplinary approach at various length scales is required to fully understand the micromechanical and metallurgical response of bearing steels widely used in industry. This review will make significant contributions to novel design methodologies and improved product design specifications to deliver the durability and reliability of bearing elements.
Influence of Self-Determination and Social Support on Post-Traumatic Growth among Living Kidney Donors: A Cross-Sectional Study
Authors: Hwang Younghui , Min Kyoungok , Oh Jihyun
Published: August 2022

Background and Objectives: Although many studies have reported that kidney donation is not physically harmful to living kidney donors, there are few studies on the psychological changes that they experience, especially post-traumatic growth. This study aimed to investigate the influence of self-determination and social support on post-traumatic growth among living kidney donors. Materials and Methods: This study used a descriptive, cross-sectional design. Data were collected from 114 living kidney donors who visited the outpatient solid organ transplant center at Seoul National University Hospital. The data were analyzed using the t-test, one-way analysis of variance, and stepwise multiple regression. Results: The results showed that the mean for post-traumatic growth of living kidney donors was 4.24 (0.81), a level higher than the midpoint. The factors affecting total post-traumatic growth were the relatedness of self-determination, the social support of their significant other, and donor type. In particular, the relatedness of self-determination was a significant factor affecting changed perceptions of self, relating to others, and spiritual change, subscales of post-traumatic growth. Additionally, the social support of donors’ significant others was a significant factor affecting relating to others and new possibilities, subscales of post-traumatic growth. Conclusions: Healthcare providers should endeavor to help living kidney donors experience post-traumatic growth, which can be facilitated by improving their self-determination and social support.
Performance Analysis of a CSFH-Based Microgripper: Analytical Modeling and Simulation
Authors: Yallew Teferi Sitotaw , Belfiore Nicola Pio , Bagolini Alvise , Pantano Maria F.
Published: August 2022

Microgrippers are promising tools for micro-manipulation and characterization of cells. In this paper, a biocompatible electro-thermally actuated microgripper with rotary capacitive position sensor is presented. To overcome the limited displacement possibilities usually provided by electrothermal actuators and to achieve the desired tweezers output displacement, conjugate surface flexure hinges (CSFH) are adopted. The microgripper herein reported can in principle manipulate biological samples in the size range between 15 and 120 µm. A kinematics modeling approach based on the pseudo-rigid-body-method (PRBM) is applied to describe the microgripper’s working mechanism, and analytical modeling, based on finite elements method (FEM), is used to optimize the electrothermal actuator design and the heat dissipation mechanism. Finally, FEM-based simulations are carried out to verify the microgripper, the electrothermal actuator and heat dissipation mechanism performance, and to assess the validity of the analytical modeling.
Exploring the Role of DARPP-32 in Addiction: A Review of the Current Limitations of Addiction Treatment Pathways and the Role of DARPP-32 to Improve Them
Authors: Greener Megan R. , Storr Sarah J.
Published: August 2022

We are amidst a global addiction crisis, yet stigmas surrounding addiction counterintuitively prevail. Understanding and appreciating the neurobiology of addiction is essential to dissolve this stigma and for the development of new pharmacological agents to improve upon currently narrow therapeutic options. This review highlights this and evaluates dopamine-and-cAMP-regulated phosphoprotein, Mr 32 kDa (DARPP-32) as a potential target to treat various forms of substance abuse. Despite the proven involvement of DARPP-32 in addiction pathophysiology, no robust investigations into compounds that could pharmacologically modulate it have been carried out. Agents capable of altering DARPP-32 signalling in this way could prevent or reverse drug abuse and improve upon currently substandard treatment options.
The Therapeutic Relevance of Urolithins, Intestinal Metabolites of Ellagitannin-Rich Food: A Systematic Review of In Vivo Studies
Authors: Tow Wai-Kit , Chee Pui-Ying , Sundralingam Usha , Palanisamy Uma Devi
Published: August 2022

The therapeutic effects of food rich in ellagitannins have been established to stem from its microbial metabolite, urolithin. Over the past decade, there has been a growing trend in urolithin research pertaining to its pharmacological properties. The purpose of this systematic review is to collate and synthesise all available data on urolithin’s therapeutic ability, to highlight its potential as a pharmaceutical agent, and prospective direction on future research. Methods: This systematic review was written based on the PRISMA guideline and was conducted across Ovid via Embase, Ovid MEDLINE, Cochrane Central Register for Controlled Trials, and Web of Science Core Collection. Results: A total of 41 animal studies were included in this systematic review based on the appropriate keyword. The included studies highlighted the neuroprotective, anti-metabolic disorder activity, nephroprotective, myocardial protective, anti-inflammatory, and musculoskeletal protection of urolithin A, B, and its synthetic analogue methylated urolithin A. The Sirt1, AMPK, and PI3K/AKT/mTOR signalling pathways were reported to be involved in the initiation of autophagy and mitochondrial biogenesis by urolithin A. Conclusions: This review methodically discusses the therapeutic prospects of urolithins and provides scientific justification for the potential development of urolithin A as a potent natural mitophagy inducer for anti-ageing purposes.
Augmented Expression of the IL3RA/CD123 Gene in MLL/KMT2A-Rearranged Pediatric AML and Infant ALL
Authors: Qazi Sanjive , Uckun Fatih M.
Published: August 2022

Here, we evaluated transcript-level IL3RA/CD123 expression in mixed lineage leukemia 1 (MLL) gene/KMT2A-rearranged (MLL-R+) vs. MLL-R− pediatric AML as well as infant ALL by comparing the archived datasets of the transcriptomes of primary leukemic cells from the corresponding patient populations. Our studies provide unprecedented evidence that IL3RA/CD123 expression exhibits transcript-level amplification in MLL-R+ pediatric AML and infant ALL cells. IL3RA was differentially upregulated in MLL-AF10+ (2.41-fold higher, p-value = 4.4 × 10−6) and MLL-AF6+ (1.83-fold higher, p-value = 9.9 × 10−4) but not in MLL-AF9+ cases compared to other pediatric AML cases. We also show that IL3RA/CD123 expression is differentially amplified in MLL-AF4+ (1.76-fold higher, p-value = 2.1 × 10−4) as well as MLL-ENL+ infant ALL (1.43-fold higher, p-value = 0.055). The upregulated expression of IL3RA/CD123 in MLL-R+ pediatric AML and infant ALL suggests that CD123 may be a suitable target for biotherapy in these high-risk leukemias.
Network Pharmacology of Adaptogens in the Assessment of Their Pleiotropic Therapeutic Activity
Authors: Panossian Alexander , Efferth Thomas
Published: August 2022

The reductionist concept, based on the ligand–receptor interaction, is not a suitable model for adaptogens, and herbal preparations affect multiple physiological functions, revealing polyvalent pharmacological activities, and are traditionally used in many conditions. This review, for the first time, provides a rationale for the pleiotropic therapeutic efficacy of adaptogens based on evidence from recent gene expression studies in target cells and where the network pharmacology and systems biology approaches were applied. The specific molecular targets and adaptive stress response signaling mechanisms involved in nonspecific modes of action of adaptogens are identified.
Licochalcone B, a Natural Autophagic Agent for Alleviating Oxidative Stress-Induced Cell Death in Neuronal Cells and Caenorhabditis elegans Models
Authors: Qu Liqun , Wu Jianhui , Tang Yong , Yun Xiaoyun , Lo Hang Hong , Yu Lu , Li Wenhua , Wu Anguo , Law Betty Yuen Kwan
Published: August 2022

Autophagy has been implicated in the regulation of neuroinflammation and neurodegenerative disorders. Licochalcone B (LCB), a chalcone from Glycyrrhiza inflata, has been reported to have anti-cancer, anti-oxidation and anti-β–amyloid fibrillation effects; however, its effect in autophagy remain un-investigated. In the current study, the potential neuro-protective role of LCB in terms of its anti-oxidative, anti-apoptotic, and autophagic properties upon oxidative stress-induced damage in neuronal cells was investigated. With the production of reactive oxygen species (ROS) as a hallmark of neuroinflammation and neurodegeneration, hydrogen peroxide (H2O2) was adopted to stimulate ROS-induced cell apoptosis in PC-12 cells. Our findings revealed that LCB reduced cell cytotoxicity and apoptosis of PC-12 cells upon H2O2-stimulation. Furthermore, LCB increased the level of the apoptosis-associated proteins caspase-3 and cleaved caspase-3 in H2O2-induced cells. LCB effectively attenuated the level of oxidative stress markers such as MDA, SOD, and ROS in H2O2-induced cells. Most importantly, LCB was confirmed to possess its anti-apoptotic effects in H2O2-induced cells through the induction of ATG7-dependent autophagy and the SIRT1/AMPK signaling pathway. As a novel autophagic inducer, LCB increased the level of autophagy-related proteins LC3–II and decreased p62 in both neuronal cells and Caenorhabditis elegans (C. elegans) models. These results suggested that LCB has potential neuroprotective effects on oxidative damage models via multiple protective pharmacological mechanisms.
The Effect on Hemostasis of Gelatin-Graphene Oxide Aerogels Loaded with Grape Skin Proanthocyanidins: In Vitro and In Vivo Evaluation
Authors: Borges-Vilches Jessica , Aguayo Claudio , Fernández Katherina
Published: August 2022

Using in vitro and in vivo models, this study investigated the hemostatic potential to control bleeding of both unloaded gelatin-graphene oxide aerogels and the same loaded with proanthocyanidins (PAs) from Vitis vinifera grape skin extract. Our results showed that the physicochemical and mechanical properties of the aerogels were not affected by PA inclusion. In vitro studies showed that PA-loaded aerogels increased the surface charge, blood absorption capacity and cell viability compared to unloaded ones. These results are relevant for hemostasis, since a greater accumulation of blood cells on the aerogel surface favors aerogel–blood cell interactions. Although PAs alone were not able to promote hemostasis through extrinsic and intrinsic pathways, their incorporation into aerogels did not affect the in vitro hemostatic activity of these composites. In vivo studies demonstrated that both aerogels had significantly increased hemostatic performance compared to SpongostanTM and gauze sponge, and no noticeable effects of PA alone on the in vivo hemostatic performance of aerogels were observed; this may have been related to its poor diffusion from the aerogel matrix. Thus, PAs have a positive effect on hemostasis when incorporated into aerogels, although further studies should be conducted to elucidate the role of this extract in the different stages of hemostasis.
Biopolymeric Prodrug Systems as Potential Antineoplastic Therapy
Authors: Chis Adriana Aurelia , Arseniu Anca Maria , Morgovan Claudiu , Dobrea Carmen Maximiliana , Frum Adina , Juncan Anca Maria , Butuca Anca , Ghibu Steliana , Gligor Felicia Gabriela , Rus Luca Liviu
Published: August 2022

Nowadays, cancer represents a major public health issue, a substantial economic issue, and a burden for society. Limited by numerous disadvantages, conventional chemotherapy is being replaced by new strategies targeting tumor cells. In this context, therapies based on biopolymer prodrug systems represent a promising alternative for improving the pharmacokinetic and pharmacologic properties of drugs and reducing their toxicity. The polymer-directed enzyme prodrug therapy is based on tumor cell targeting and release of the drug using polymer–drug and polymer–enzyme conjugates. In addition, current trends are oriented towards natural sources. They are biocompatible, biodegradable, and represent a valuable and renewable source. Therefore, numerous antitumor molecules have been conjugated with natural polymers. The present manuscript highlights the latest research focused on polymer–drug conjugates containing natural polymers such as chitosan, hyaluronic acid, dextran, pullulan, silk fibroin, heparin, and polysaccharides from Auricularia auricula.
Crystallization Thermodynamics of α-Lactose Monohydrate in Different Solvents
Authors: Guan Youliang , Yang Zujin , Wu Kui , Ji Hongbing
Published: August 2022

It is common to find that some of the lactose in dairy powders and pharmaceutical tablets is present in the unstable amorphous state. Therefore, their crystallization thermodynamics in different solvents are particularly important. In this paper, the solubility of α-lactose monohydrate (α-LM) in 15 mono-solvents such as ethanol, isopropanol, methanol, 1-propanol, 1-butanol, 2-butanol, isobutanol, 1-pentanol, isoamylol, 1-hexanol, 1-heptanol, 1-octanol, propanoic acid, acetonitrile, and cyclohexanone was evaluated by using the gravimetric method in the temperature ranges from 274.05 K to 323.05 K at constant pressure (1 atm). In the given temperature range, the solubility of α-LM in these solvents increased with the rising of temperature, the highest solubility of α-LM was found in methanol (2.37 × 104), and the lowest was found in 1-hexanol (0.80 × 105). In addition, the increase of α-LM solubility in isopropanol was the largest. The sequence at 298.15 K was: methanol > 1-butanol > isopropanol > ethanol > 1-propanol > 1-heptanol > isobutanol > propionic acid > 1-pentanol > 1-octanol > acetonitrile > isoamylol > 2-butanol > cyclohexanone > 1-hexanol. Solvent effect analysis shows that the properties of α-LM are more important than those of solvents. The Apelblat equation, λh equation, Wilson model, and NRTL model were used to correlate the experimental values. The root-mean-square deviation (RMSD) and relative average deviation (RAD) of all models were less than 2.68 × 10−2 and 1.41 × 10−6, respectively, implying that the fitted values of four thermodynamic models all agreed well with the experimental values. Moreover, the thermodynamic properties of the dissolution process (i.e., dissolution Gibbs free energy (ΔdisG), molar enthalpy (ΔdisH), and molar entropy (ΔdisS)) for α-LM in selected solvents were determined. The results indicate that ΔdisH/(J/mol) (from 0.2551 to 6.0575) and ΔdisS/(J/mol/K) (from 0.0010 to 0.0207) of α-LM in these solvents are all positive, and the values of ΔdisH and ΔdisS. ΔdisG/(J/mol) (from −0.0184 to −0.6380) are all negative. The values were observed to decrease with rising temperatures, implying that α-LM dissolution is an endothermic, entropy-driven, and spontaneous process. The solid–liquid equilibrium data and dissolution thermodynamics of α-LM were obtained, which provide a basis for industrial production.
Role of Nrf2, STAT3, and Src as Molecular Targets for Cancer Chemoprevention
Authors: Ahsan Haseeb , Islam Salman Ul , Ahmed Muhammad Bilal , Lee Young Sup
Published: August 2022

Cancer is a complex and multistage disease that affects various intracellular pathways, leading to rapid cell proliferation, angiogenesis, cell motility, and migration, supported by antiapoptotic mechanisms. Chemoprevention is a new strategy to counteract cancer; to either prevent its incidence or suppress its progression. In this strategy, chemopreventive agents target molecules involved in multiple pathways of cancer initiation and progression. Nrf2, STAT3, and Src are promising molecular candidates that could be targeted for chemoprevention. Nrf2 is involved in the expression of antioxidant and phase II metabolizing enzymes, which have direct antiproliferative action as well as indirect activities of reducing oxidative stress and eliminating carcinogens. Similarly, its cross-talk with NF-κB has great anti-inflammatory potential, which can be utilized in inflammation-induced/associated cancers. STAT3, on the other hand, is involved in multiple pathways of cancer initiation and progression. Activation, phosphorylation, dimerization, and nuclear translocation are associated with tumor cell proliferation and angiogenesis. Src, being the first oncogene to be discovered, is important due to its convergence with many upstream stimuli, its cross-talk with other potential molecular targets, such as STAT3, and its ability to modify the cell cytoskeleton, making it important in cancer invasion and metastasis. Therefore, the development of natural/synthetic molecules and/or design of a regimen that can reduce oxidative stress and inflammation in the tumor microenvironment and stop multiple cellular targets in cancer to stop its initiation or retard its progression can form newer chemopreventive agents.
Lactoferrin Decreases Enterotoxigenic Escherichia coli-Induced Fluid Secretion and Bacterial Adhesion in the Porcine Small Intestine
Authors: Dierick Matthias , Ongena Ruben , Vanrompay Daisy , Devriendt Bert , Cox Eric
Published: August 2022

Enterotoxigenic Escherichia coli (ETEC) infections are one of the most prevalent causes of post-weaning diarrhea in piglets, resulting in morbidity, mortality and elevated use of antibiotics. The emergence and further spread of antimicrobial resistance together with the growing demand for high quality animal protein requires the identification of novel alternatives for antimicrobials. A promising alternative is lactoferrin, as we previously showed that it can both inhibit the growth and degrade bacterial virulence factors of porcine ETEC strains in vitro. Aiming to confirm these findings in vivo, we performed a small intestinal segment perfusion experiment in piglets. Here, we showed that lactoferrin could not only decrease ETEC-induced fluid secretion, but also their ability to colonize the small intestinal epithelium. Furthermore, while ETEC infection induced pro-inflammatory cytokine mRNA expression in this experiment, lactoferrin was not able to counteract these responses. In addition, a bacterial motility assay showed that lactoferrin can reduce the motility of ETEC. Our findings further support the use of lactoferrin as an alternative for antimicrobials and also show its potential for the prevention of ETEC infections in pigs.
Cutting-Edge Delivery Systems and Adjuvants in Tolerogenic Vaccines: A Review
Authors: Puricelli Chiara , Boggio Elena , Gigliotti Casimiro Luca , Stoppa Ian , Sutti Salvatore , Rolla Roberta , Dianzani Umberto
Published: August 2022

Conventional therapies for immune-mediated diseases, including autoimmune disorders, transplant reactions, and allergies, have undergone a radical evolution in the last few decades; however, they are still not specific enough to avoid widespread immunosuppression. The idea that vaccine usage could be extended beyond its traditional immunogenic function by encompassing the ability of vaccines to induce antigen-specific tolerance may revolutionize preventive and therapeutic strategies in several clinical fields that deal with immune-mediated disorders. This approach has been supported by improved data relating to the several mechanisms involved in controlling unwanted immune responses and allowing peripheral tolerance. Given these premises, several approaches have been developed to induce peripheral tolerance against the antigens that are involved in the pathological immune response, including allergens, autoantigens, and alloantigens. Technological innovations, such as nucleic acid manipulation and the advent of micro- and nanoparticles, have further supported these novel preventive and therapeutic approaches. This review focuses on the main strategies used in the development of tolerogenic vaccines, including the technological issues used in their design and the role of “inverse adjuvants”. Even though most studies are still limited to the preclinical field, the enthusiasm generated by their results has prompted some initial clinical trials, and they show great promise for the future management of immune-mediated pathological conditions.
Studies on the Interaction of Poly(phenylene methylene) with Silver(I) and Hexacarbonylchromium(0)
Authors: Guichard Xavier H. , Braendle Andreas , Niederberger Markus , Caseri Walter
Published: August 2022

Complexes of poly(phenylene methylene) (PPM) with silver(I) ions and tricarbonylchromium(0) moieties, respectively, were synthesized. 13C NMR spectra indicate interaction of phenylene groups with silver(I) and chromium(0), and peak broadening implies dynamic behavior of the silver(I) complexes, with all phenylene groups temporarily involved in coordination, in contrast to the chromium complexes. About 5–10% of the phenylene groups are coordinated to metal atoms. 1H NMR and IR spectra, in the case of chromium(0), and the solubility of silver salts in the presence of PPM provide further evidence of coordination. The complexes are soluble in chloroform, but the silver complexes decay in tetrahydrofuran (second-order kinetics were observed in an example). The photoluminescence (fluorescence) of PPM is maintained upon complexation, although coordination of silver(I) seems to favor the so-called blue phase of PPM relative to the green phase by a factor of approximately two in PL spectra. The pronounced absorption of the tricarbonylchromium(0) units interferes with the blue phase, which almost disappears at a concentration of 50 mg/mL in PLE spectra, whereas the emission maximum of the green phase is hardly affected. This leads to a confinement of the emitted wavelength range of PPM. Thus, the perceived optical emission of PPM can be modified by coordinated entities.
Finite Element Simulation and Experimental Research on Uniformity Regulation of Microwave Heating of Composite Materials
Authors: Guan Chenglong , Zhan Lihua , Yao Shunming
Published: August 2022

As an attractive alternative to the traditional autoclave curing process, microwave curing has been widely used for manufacturing high-performance composites. However, the nonuniform temperature distribution during composite curing is the main problem faced by the microwave curing process, which limits its application in the aerospace industry. This paper studied the regulating effects of cavity structure and mechanical optimization methods on the uniformity of the microwave field by numerical analysis and finite element simulation, and an octagonal microwave heating device with multi-microwave generators, mode stirrers and mobile platform was developed independently and the experimental verification were finally carried out. The results showed that compared with the traditional heating device, the T800/602 carbon fiber reinforced composite laminates cured in the regulating device of microwave heating uniformity established in this paper had more uniform temperature field distribution, a more synchronous curing process and lower residual stresses.
Improved Rotational Foam Molding Properties of Tailored Polyethylene Blends with Higher Crystallization Temperature and Viscosity-Temperature Sensitivity
Authors: Chen Xuelian , Huang Qigu
Published: August 2022

Rotational foam molding has attracted more and more attention due to the inexpensive machines required, low residual stresses, and flexible design for special and high-value-added applications. However, it is a great challenge to control cell sizes and morphology because of its coalesce and collapse during prolonged heating or at different temperatures. A novel tailored polyethylene blend with a unique chain structure for rotational foam molding was creatively proposed and demonstrated, and the effects of crystallization temperature and viscosity–temperature sensitivity on foaming were also investigated. The polyethylene blends with few chain branches in the low-molecular-weight part and many chain branches in the high-molecular-weight part effectively improved the crystallization temperature and the viscosity–temperature sensitivity for better prevention of coalesce and collapse during the foam-shaping stage.
A Polarization Stacking Method for Optimizing Time-Series Interferometric Phases of Distributed Scatterers
Authors: Shen Peng , Wang Changcheng , Hu Jun
Published: August 2022

For time-series interferometric phases optimization of distributed scatterers (DSs), the SqueeSAR technology used the phase linking (PL) to extract the equivalent single-master (ESM) interferometric phases from the multilooking time-series coherence matrix. The Cramer–Rao lower bound (CRLB) for the PL describes the highest achievable estimation accuracy of the ESM phases, which depends on the number of looks and the time-series coherence magnitude matrix. With the abundance of time-series polarimetric SAR data, many scholars have studied the coherence magnitude-based polarimetric optimization methods for optimizing the DS’s time-series interferometric phases, for example, the widely-used exhaustive search polarimetric optimization (ESPO) algorithm. However, the traditional polarimetric optimization methods select the boundary extremums of the coherence region (CR) as the optimized complex coherence, which is usually biased from the free-noise one. Currently, in the polarimetric InSAR (PolInSAR) technology, Shen et al. innovatively considered polarimetric information as statistical samples and proposed the total power (TP) coherency matrix construction method for increasing the number of looks and reducing the interferometric phase noise. Therefore, to optimize the time-series interferometric phases for DS, this paper proposes performing a polarization stacking and extending the PolInSAR TP construction to the time-series PolInSAR (TSPolInSAR) data configuration, called the time-series TP (TSTP) method. Simulated and real experiments prove that the new TSTP construction method has better performance and higher efficiency than the single polarimetric and the traditional ESPO algorithms.
Tracking Individual Scots Pine (Pinus sylvestris L.) Height Growth Using Multi-Temporal ALS Data from North-Eastern Poland
Authors: Kozniewski Marcin , Kolendo Łukasz , Ksepko Marek , Chmur Szymon
Published: August 2022

In this study, we analyzed the change in tree height of 2594 Scots pine (Pinus sylvestris L.) trees with respect to the distribution among different forest sites: HCfs—hydrogenic coniferous forest site; MCfs—mineral coniferous forest site; MMfs—mineral mixed forest site. We obtained tree height information from three independent airborne laser scanning (ALS) point clouds acquired in north-eastern Poland over a 5-year interval in 2007, 2012, and 2017 using verified tree crown segments. We performed a comparative analysis of digital terrain models (DTMs) calculated from analyzed point clouds, indicating that the highest elevation differences were observed in the case of data from 2007. The analyses showed that tree growth varies significantly depending on the forest site productivity and the stage of tree development, which was described as initial tree height instead of age—commonly used in such studies. In conclusion, it is possible to indicate the significant information potential of using multitemporal ALS data to track individual tree height changes. These field data, combined with meteorological data, can be successfully used to predict changes in the abundance of stands depending on the forest site productivity. We have built Scots pine growth models for each forest site, which indicates that it is possible to predict the change in the tree stand height.
Multicriteria Accuracy Assessment of Digital Elevation Models (DEMs) Produced by Airborne P-Band Polarimetric SAR Tomography in Tropical Rainforests
Authors: El Hage Mhamad , Villard Ludovic , Huang Yue , Ferro-Famil Laurent , Koleck Thierry , Le Toan Thuy , Polidori Laurent
Published: August 2022

The penetration capability of P-band radar waves through dense vegetation, along with the ability of tomography to separate the contributions of different layers in a vertical reflectivity profile, make P-band radar tomography a promising tool for digital terrain modeling in forested areas, specifically in dense tropical forests under which terrain topography remains poorly known. This paper aims to assess the overall quality of a digital terrain model (DTM) produced using tomographic processing of airborne P-band SAR imagery acquired during the TropiSAR campaign in French Guiana. Many quality descriptors are used to evaluate the quality of this DTM. Position and slope accuracies are computed based on a lidar DTM considered as the reference, and the impact of several parameters on these accuracies is studied, namely, slope, slope orientation, off-nadir angle and local incidence angle. The realism of the landforms is also studied according to geomorphological criteria. The results of this multicriteria accuracy assessment show the high potential of P-band SAR tomography in depicting the topography under forests, despite the intrinsic limitations related to the slant range geometry: the absolute elevation error is around 2 m; the slope is overestimated with an error of about 16°, mainly due to a processing artifact for which easy and direct solutions exist. Indeed, this error is equal to about 3° in flat artifact-free areas. These errors vary depending on the acquisition parameters and the local topography. The shapes are globally well preserved. These results are also discussed in the frame of the upcoming BIOMASS mission developed by the European Space Agency (ESA) and expected to be launched in 2024.
Reconstructing the Three-Dimensional Structure of Loop Current Rings from Satellite Altimetry and In Situ Data Using the Gravest Empirical Modes Method
Authors: Meunier Thomas , Pérez-Brunius Paula , Bower Amy
Published: August 2022

The three-dimensional structure of Gulf of Mexico’s warm-core rings, detaching from the Loop Current, is investigated using satellite altimetry and a large set of ARGO float profiles. Reconstruction of the Loop Current rings (LCRs) vertical structure from sea surface height observations is made possible by the use of the gravest empirical modes method (GEM). The GEMs are transfer functions that associate a value of temperature and salinity for each variable pair {dynamic height; pressure}, and are computed by estimating an empirical relationship between dynamic height and the vertical thermohaline structure of the ocean. Between 1993 and 2021, 40 LCRs were detected in the altimetry and their three-dimensional thermohaline structure was reconstructed, as well as a number of dynamically relevant variables (geostrophic and cyclogeostrophic velocity, relative vorticity, potential vorticity, available potential energy and kinetic energy density, etc.). The structure of a typical LCR was computed by fitting an analytical stream function to the LCRs dynamic height signature and reconstructing its vertical structure with the GEM. The total heat and salt contents and energy of each LCR were computed and their cumulative effect on the Gulf of Mexico’s heat, salt and energy balance is discussed. We show that LCRs have a dramatic impact on these balances and estimate that residual surface heat fluxes of −13 W m−2 are necessary to compensate their heat input, while the fresh water outflow of the Mississippi river approximately compensates for their salt excess input. An average energy dissipation of O [10−10–10−9] W kg−1 would be necessary to balance their energy input.
Characteristics and Formation Conditions of Thin Phytoplankton Layers in the Northern Gulf of Mexico Revealed by Airborne Lidar
Authors: Yang Yichen , Pan Hangkai , Zheng Dekang , Zhao Hongkai , Zhou Yudi , Liu Dong
Published: August 2022

The thin layers in the ocean are temporally-coherent aggregations of phytoplankton with high concentrations at small vertical scales, presenting important hotspots of ecological activity. Lidar could identify thin phytoplankton layers at a large spatial scale due to its capabilities of profile detection with a high efficiency. However, studies that linked thin layers to environmental factors are few, which limits our understanding of the layer formation mechanism. This paper investigates the characteristics and formation conditions of thin phytoplankton layers in the northern Gulf of Mexico using airborne lidar. The results depict that the chlorophyll concentration determines the formation probability of the phytoplankton layer. The layer is mainly formed at concentrations less than 6 mg m−3 and mostly distributed at 2 mg m−3. In addition, layer thicknesses were within 5 m and layer depths were mainly in the range of 10–15 m. Layer depths in the nearshore region were shallower than those in the offshore region. We conclude that the characteristics and formation conditions of the thin phytoplankton layers depend on the nutrients and light that are related to the seabed topography, turbidity, eddies and upwelling. The findings of this paper will enhance the understanding of layer formation mechanisms.
Soil Moisture Estimation Based on Polarimetric Decomposition and Quantile Regression Forests
Authors: Zhang Li , Lv Xiaolei , Wang Rui
Published: August 2022

The measurement of surface soil moisture (SSM) assists in making agricultural decisions, such as precision irrigation and flooding or drought predictions. The critical challenge for SSM estimation in vegetation-covered areas is the coupling between vegetation and surface scattering. This study proposed an SSM estimation method based on polarimetric decomposition and quantile regression forests (QRF) to overcome this problem. Model-based polarimetric decomposition separates volume scattering, double-bounce scattering, and surface scattering, while eigenvalue-based polarimetric decomposition provides additional parameters to describe the scattering mechanism. The combined use of these parameters explains the polarimetric SAR scattering information from multiple perspectives, such as vegetation, surface roughness, and SSM. As different crops differ in morphology and structure, it is essential to investigate the potential of varying polarimetric parameters to estimate SSM in areas covered by different crops. QRF, a regression method applicable to high-dimensional predictor variables, is used to estimate SSM from these parameters. In addition to the SSM estimates, QRF can also provide the predicted uncertainty intervals and quantify the importance of the different parameters in the SSM estimates. The performance of QRF in SSM estimation was tested using data from the soil moisture active passive validation experiment 2012 (SMAPVEX12) and compared with copula quantile regression (CQR). The SSM estimated by the proposed method was consistent with the in situ SSM, with the root-mean-square-error ranging from 0.037 cm3/cm3 to 0.079 cm3/cm3 and correlation coefficients ranging from 0.745 to 0.905. Meanwhile, the method proposed in this study can provide both the uncertainty of SSM estimation and the importance of different polarimetric parameters.