bluuebunny/crossref_metadata_2025 huggingface dataset:
Lymphocytes regulate expression of the SARS‐CoV‐2 cell entry factor ACE2 in the pancreas of T2DM patients
Authors: Zhang Peng , Zheng Chang‐Bo , Liu Xiao‐Yu , Zhang Xiaowei , Huang Lingyan , Zeng Xianhai
Published: April 2023

Aims

COVID‐19 patients with type 2 diabetes mellitus (T2DM) show both poorer clinical outcomes and have an increased risk of death. SARS‐CoV‐2 virus infection requires simultaneous expression of the SARS‐CoV‐2 cell entry factors angiotensin‐converting enzyme 2 (ACE2) and transmembrane protease serine type 2 (TMPRSS2) in the same cell. The aim of the study was to explore the underlying mechanisms of a COVID‐19 infection in patients with T2DM.

Methods

The distribution and expression of AEC2 and TMPRSS2 in different pancreatic cell types in clinical samples of T2DM patients and diabetic mouse models were analysed by single‐cell sequencing, bioinformatics analysis and basic experiments.

Results

The results showed that ACE2 and TMPRSS2 are expressed in the ducts of the human pancreas. These findings suggest that SARS‐CoV‐2 can infect ductal cells in vivo through ACE2 and TMPRSS2. T2DM can promote the co‐expression of ACE2 and TMPRSS2 in exocrine ducts, including in the human pancreas. We hypothesize that ACE2 expression levels are associated with increased numbers of lymphocytes in vivo.

Conclusions

Increased blood glucose levels are associated with increased ACE2 expression and an increased number of lymphocytes. At the same time, lymphocytes can promote ACE2 expression.
Young adults' retrospective reports of family cohesion, parental differential treatment, and sibling relationships
Authors: Zhou Weimiao , Woszidlo Alesia
Published: April 2023

Objective

Using young adults' retrospective reports, the current study examines the moderating effects of parental differential treatment (PDT) on the association between family cohesion and sibling relationship quality during adolescence.

Background

Sibling relationships in adolescence carry great implications for individual development. However, little is known about the potential interactions between family cohesion and PDT on sibling affection and hostility.

Method

Retrospective data were collected from 325 young adults (M = 19.50 years, SD = 1.25) who recalled family of origin experiences with parents and a target sibling closest to their age.

Results

Family cohesion was positively associated with sibling affection and negatively associated with sibling hostility. Additionally, fathers' differential control attenuated the relationship between cohesion and sibling affection and both mothers' and fathers' differential affection attenuated the relationship between cohesion and sibling hostility.

Conclusion

Findings on the main effects suggest that perceived family cohesion is a crucial factor that promotes better sibling relationships (i.e., higher affection and lower hostility). However, offspring's perceptions of PDT can affect the relationship between family cohesion and sibling relationship quality, such that extreme levels of parents' (especially fathers') differential affection and control weaken the association. The significant moderation emerged with differences in parental sex (i.e., mother and father) and PDT dimensions (i.e., affection and control).

Implications

To help siblings engage in more affectionate and fewer hostile interactions, parents (especially fathers) should consciously work to provide equal treatment toward their offspring and work on providing more contextual information when they have to show differentiation.
The human phosphorylated pathway: a multienzyme metabolic assembly for l‐serine biosynthesis
Authors: Rabattoni Valentina , Marchesani Francesco , Murtas Giulia , Sacchi Silvia , Mozzarelli Andrea , Bruno Stefano , Peracchi Alessio , Pollegioni Loredano , Campanini Barbara
Published: April 2023

De novo l‐serine biosynthesis in the mammalian astrocytes proceeds via a linear, three‐step pathway (the phosphorylated pathway) catalysed by 3‐phosphoglycerate dehydrogenase (PHGDH), phosphoserine aminotransferase (PSAT) and phosphoserine phosphatase (PSP). The first reaction, catalysed by PHGDH and using the glycolytic intermediate 3‐phosphoglycerate, is strongly shifted towards the reagents, and coupling to the following step by PSAT is required to push the equilibrium towards l‐serine formation; the last step, catalysed by PSP, is virtually irreversible and inhibited by the final product l‐serine. Very little is known about the regulation of the human phosphorylated pathway and the ability of the three enzymes to organise in a complex with potential regulatory functions. Here, the complex formation was investigated in differentiated human astrocytes, by proximity ligation assay, and in vitro on the human recombinant enzymes. The results indicate that the three enzymes co‐localise in cytoplasmic clusters that more stably engage PSAT and PSP. Although in vitro analyses based on native PAGE, size exclusion chromatography and cross‐linking experiments do not show the formation of a stable complex, kinetic studies of the reconstituted pathway using physiological enzyme and substrate concentrations support cluster formation and indicate that PHGDH catalyses the rate‐limiting step while PSP reaction is the driving force for the whole pathway. The enzyme agglomerate assembly of the phosphorylated pathway (the putative ‘serinosome’) delivers a relevant level of sophistication to the control of l‐serine biosynthesis in human cells, a process strictly related to the modulation of the brain levels of d‐serine and glycine, the main co‐agonists of N‐methyl‐d‐aspartate receptors and various pathological states.
Elemental behaviors of γ‐irradiated borosilicate glass as a vitrification model
Authors: Zhang Jiandong , Xia Xiaoyu , Zeng Fanrong , Xi Xiaochong , Zhang Xiaoyang , Pan Yuhe , Sun Yuxi , Jia Wenbao , Peng Haibo
Published: April 2023

Borosilicate glass has been extensively studied due to its unique properties of solidifying high‐level radioactive waste (HLW). However, the responses of borosilicate glass under γ irradiation are not fully understood. In this work, NBS9 and NBS10 glass were irradiated by γ‐rays at absorbed doses of 8 kGy and 800 kGy, respectively. Scanning electronic microscopy, energy dispersive X‐ray, and time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) were used to observe the surface morphology and elemental distributions. The results show that the borosilicate glass remains stable until the absorbed dose was up to 800 kGy. At 800 kGy, the samples precipitate particles composed of Na and O on the surface. Na and B near the surface are significantly reduced under γ‐rays irradiation. The results indicate that the effects of γ irradiation on glass vitrification are obvious with certain accumulated doses. The changes of glass structures and elemental distributions by γ‐ray irradiation are also dependent on glass compositions.
Tissue‐resident memory T cells and lung immunopathology
Authors: Cheon In Su , Son Young Min , Sun Jie
Published: April 2023

Rapid reaction to microbes invading mucosal tissues is key to protect the host against disease. Respiratory tissue‐resident memory T (TRM) cells provide superior immunity against pathogen infection and/or re‐infection, due to their presence at the site of pathogen entry. However, there has been emerging evidence that exuberant TRM‐cell responses contribute to the development of various chronic respiratory conditions including pulmonary sequelae post‐acute viral infections. In this review, we have described the characteristics of respiratory TRM cells and processes underlying their development and maintenance. We have reviewed TRM‐cell protective functions against various respiratory pathogens as well as their pathological activities in chronic lung conditions including post‐viral pulmonary sequelae. Furthermore, we have discussed potential mechanisms regulating the pathological activity of TRM cells and proposed therapeutic strategies to alleviate TRM‐cell‐mediated lung immunopathology. We hope that this review provides insights toward the development of future vaccines or interventions that can harness the superior protective abilities of TRM cells, while minimizing the potential for immunopathology, a particularly important topic in the era of coronavirus disease 2019 (COVID‐19) pandemic.
Evaluation of a theory‐informed implementation of a nursing discharge teaching intervention for older adults
Authors: Pellet Joanie , Weiss Marianne , Mabire Cedric
Published: April 2023

Aims

To evaluate the implementation process of a multi‐site trial of a novel discharge teaching intervention.

Design

Hybrid type 3 trial.

Methods

A discharge teaching intervention for older adults was implemented in medical units from August 2020 to August 2021 with 30 nurse participants. The implementation process was guided by behaviour change frameworks. Outcome data comprised determinants of nurses' behaviours related to teaching and the acceptability, appropriateness, feasibility of the intervention and frequency of teaching activities received by the participants. This study adheres to StaRI and TIDieR reporting guidelines.

Results

Twelve of 18 determinants of nurses' behaviour domains improved post‐implementation. Being trained reinforced nurses' knowledge and skills in patient teaching. Practicing the intervention increased their awareness on the gaps between evidence‐based teaching principles and their actual practice. The intervention was considered acceptable and moderately appropriate and feasible.

Conclusion

A theoretically informed implementation process can influence nurses' perceptions and behaviours related to discharge teaching by targeting specific behaviour domains. Practice change to improve discharge teaching will require organizational support from nursing management.

No Patient or Public Contribution

Although the conceptual foundations of the intervention tested in this study were informed by the priorities and experience of patients, this population was not directly involved in the design and conduct of the study.

Trial registration

ClinicalTrials.gov:NCT04253665.
Local education expenditures and educational inequality in China
Authors: Fan Jianyong , Huang Jian , Sessions John G. , Ye Jingjing
Published: April 2023

We investigate the relationship between education funding and educational inequality across Chinese prefectures. The decentralisation of education in China has created substantial variations in government educational expenditures, both over time and across regions. We propose that these variations relate to the budget preferences of local governors. These are age dependent with younger officials more inclined to invest in large and quantifiable infrastructure projects rather than public service provision. This provides a source of exogenous variation in local fiscal efforts to provide public education and thus permits quasi‐experimental evaluation through instrumental variable identification. Our results suggest that increased education spending is linked with lower educational inequality. Moreover, we find strong evidence of heterogeneity ‐ the magnitude of the effect is diminishing with the degree of local fiscal autonomy.
Climate change‐induced ecosystem disturbance: a review on sclerophyllous and semi‐deciduous forests in Tunisia
Authors: Touhami I. , Rzigui T. , Zribi L. , Ennajah A. , Dhahri S. , Aouinti H. , Elaieb M. T. , Fkiri S. , Ghazghazi H. , Khorchani A. , Candelier K. , Khaldi A. , Khouja M. L.
Published: April 2023

According to the sixth assessment report of the Intergovernmental Panel on Climate Change (IPCC), global climate change is now unequivocal. Tunisia, like many other countries, has been affected by climate changes, including rising temperatures, intense heatwaves, and altered precipitation regimes. Tunisia's mean annual temperatures has risen about +1.4 °C in the twentieth century, with the most rapid warming taking place since the 1970s. Drought represents a primary contributing factor to tree decline and dieback. Long‐term drought can result in reduced growth and health of trees, thereby increasing their susceptibility to insect pests and pathogens. Reported increases in tree mortality point toward accelerating global forest vulnerability under hotter temperatures and longer, more intense droughts. In order to assess the effect of these climate changes on the current state of forest ecosystems in Tunisia and their evolution, an investigative study was required. Here, we review the current state of knowledge on the effects of climate change on sclerophyllous and semi‐deciduous forest ecosystems in Tunisia. Natural disturbance during recent years, as well as the adaptability and resilience of some forest species to climate change, were surveyed. The Standardized Precipitation Evapotranspiration Index (SPEI) is a multi‐scalar drought index based on climate data that has been used to analyse drought variability. The SPEI time scale analysis showed a negative trend over the 1955–2021 period in Tunisian forest regions. In 2021, Tunisia lost 280 km2 of tree cover to fires, which is equivalent to 26% of the total lost area between 2008 and 2021. Changing climate conditions have also affected phenological parameters, with an advance in the start of the green season (SOS) of 9.4 days, a delay at the end of the green season (EOS) of 5 days, with a consequent extended duration of the green season (LOS) by an average of 14.2 days. All of these alarming findings invite us to seek adaptation strategies for forest ecosystems. Adapting forests to climate change is therefore a challenge for scientists as well as policymakers and managers.
Public perspectives on curriculum reform for truth and reconciliation in Canada
Authors: Wotherspoon Terry , Milne Emily
Published: April 2023

The implementation of school reforms to advance reconciliation with Indigenous peoples provides an opportunity to explore what Canadians think is important in framing their identities and values. This paper draws on data from a survey of public perspectives on education for reconciliation activities in two Canadian provinces, Alberta and Saskatchewan. We consider the broader community context within which schools are located. By examining public perspectives, we are able to assess how curricular initiatives related to reconciliation are understood by community members, both as a priority in itself and in relation to other key curricular areas. Informed by critical race theory, our findings suggest that reconciliation is restricted to activities that do not involve extensive change to existing curricular and ideational frameworks. These perspectives exist alongside extensive opposition to reconciliation justified by claims that Indigenous experiences and perspectives are receiving undue attention relative to more pressing educational priorities. Our findings suggest that aims to transform dominant understandings about Canadian history and identity remain far from being fulfilled.
New records of two alien plants, Juncus torreyi (Juncaceae) and Egeria densa (Hydrocharitaceae) in Korea
Authors: JUNG Jongduk , NA Hye Ryun , LEE Kyu Song , CHOI Yeongmin , CHO Woongrae , HYUN Jin-Oh
Published: April 2023

Naturalized populations of two alien plants were newly found, and we describe their morphological characteristics and habitats with photographs. One is a member of Juncaceae, Juncus torreyi Coville, and was newly found at a pool of a beach in Gangwon-do. This rush is native to North America and belongs to the sect. Ozophyllum (subgen. Juncus) according to certain morphological characteristics, such as its racemose inflorescence, the absence of floral bracteole, and unitubular leaves with perfect septa. J. torreyi is easily distinguishable from Korean rushes by its long rhizomes with swollen nodes and globular head with 25–100 flowers. Its introduction into Japan and Europe was reported, but the ecological risk associated with its over-dispersal is not known. The other alien plant is a submerged plant, Egeria densa Planch. (Hydrocharitaceae), which was found in streams in Gyeongsangbuk-do and ditches in the Busan-si area, both of which being in the watershed of the Nakdong River. Egeria densa is similar to Hydrilla verticillata (L.f.) Royle, which is native to Korea. However, it is distinguished from H. verticillata by its larger flowers and lack of overwintering organs. This alien plant is native to South America and was introduced for aquarium gardening and naturalized around the world. Egeria densa is treated as a malignant weed due to its asexual reproduction and rapid growth. Size changes and the number of populations of E. densa must be investigated.
Central and peripheral chemoreflexes in humans with treated hypertension
Authors: Sayegh Ana Luiza C. , Fan Jui‐Lin , Dawes Mathew , Paton Julian F. R. , Fisher James P.
Published: April 2023

Increased peripheral chemoreflex sensitivity is a pathogenic feature of human hypertension (HTN), while both central and peripheral chemoreflex sensitivities are reportedly augmented in animal models of HTN. Herein, we tested the hypothesis that both central and combined central and peripheral chemoreflex sensitivities are augmented in HTN. Fifteen HTN participants (68 ± 5 years; mean ± SD) and 13 normotensives (NT; 65 ± 6 years) performed two modified rebreathing protocols in which the partial pressure of end‐tidal carbon dioxide () progressively increased while the partial pressure of end‐tidal oxygen was clamped at either 150 mmHg (isoxic hyperoxia; central chemoreflex activation) or 50 mmHg (isoxic hypoxia; combined central and peripheral chemoreflex activation). Ventilation (; pneumotachometer) and muscle sympathetic nerve activity (MSNA; microneurography) were recorded, and ventilatory ( vs.  slope) and sympathetic (MSNA vs. slope) chemoreflex sensitivities and recruitment thresholds (breakpoint) were calculated. Global cerebral blood flow (gCBF; duplex Doppler) was measured, and the association with chemoreflex responses was examined. Central ventilatory and sympathetic chemoreflex sensitivities were greater in HTN than NT (2.48 ± 1.33 vs. 1.58 ± 0.42 L min−1 mmHg−1, P = 0.030: 3.32 ± 1.90 vs. 1.77 ± 0.62 a.u. mmHg−1, P = 0.034, respectively), while recruitment thresholds were not different between groups. HTN and NT had similar combined central and peripheral ventilatory and sympathetic chemoreflex sensitivities and recruitment thresholds. A lower gCBF was associated with an earlier recruitment threshold for (R2 = 0.666, P < 0.0001) and MSNA (R2 = 0.698, P = 0.004) during isoxic hyperoxic rebreathing. These findings indicate that central ventilatory and sympathetic chemoreflex sensitivities are augmented in human HTN and perhaps suggest that targeting the central chemoreflex may help some forms of HTN.

image

Key points

In human hypertension (HTN) increased peripheral chemoreflex sensitivity has been identified as a pathogenic feature, and in animal models of HTN, both central and peripheral chemoreflex sensitivities are reportedly augmented.

In this study, the hypothesis was tested that both central and combined central and peripheral chemoreflex sensitivities are augmented in human HTN.

We observed that both central ventilatory and sympathetic chemoreflex sensitivities were augmented in HTN compared to age‐matched normotensive controls, but no difference was found in the combined central and peripheral ventilatory and sympathetic chemoreflex sensitivities.

During central chemoreflex activation, the ventilatory and sympathetic recruitment thresholds were lower in those with lower total cerebral blood flow.

These results indicate a potential contributory role of the central chemoreceptors in the pathogenesis of human HTN and support the possibility that therapeutic targeting of the central chemoreflex may help some forms of HTN.
Periendoscopic management of direct oral anticoagulants: a prospective
cohort study
Authors: Radaelli Franco , Fuccio Lorenzo , Paggi Silvia , Hassan Cesare , Repici Alessandro , Rondonotti Emanuele , Semeraro Rossella , Di Leo Milena , Anderloni Andrea , Amato Arnaldo , Trovato Cristina , Bravi Ivana , Buda Andrea , de Bellis Mario , D’Angelo Valentina , Segato Sergio , Tarantino Ottaviano , Musso Alessandro , Fasoli Renato , Frazzoni Leonardo , Liverani Elisa , Fabbri Carlo , Di Giulio Emilio , Esposito Gianluca , Pigò Flavia , Iannone Andrea , Dentali Francesco
Published: April 2023

Objective

To assess the frequency of adverse events associated with
periendoscopic management of direct oral anticoagulants (DOACs) in
patients undergoing elective GI endoscopy and the efficacy and safety of
the British Society of Gastroenterology (BSG) and European Society of
Gastrointestinal Endoscopy (ESGE) recommendations (NCT
02734316).

Design

Consecutive patients on DOACs scheduled for elective GI endoscopy
were prospectively included. The timing of DOAC interruption and
resumption before and after the procedures were recorded, along with
clinical and procedural data. Procedures were stratified into low-risk
and high-risk for GI-related bleeding, and patients into low-risk and
high-risk for thromboembolic events. Patients were followed-up for 30
days for major and clinically relevant non-major bleeding events
(CRNMB), arterial and venous thromboembolism and death.

Results

Of 529 patients, 38% and 62% underwent high-risk and low-risk
procedures, respectively. There were 45 (8.5%; 95% CI 6.3% to 11.2%)
major or CRNMB events and 2 (0.4%; 95% CI 0% to 1.4%) thromboembolic
events (transient ischaemic attacks). Overall, the incidence of bleeding
events was 1.8% (95% CI 0.7% to 4%) and 19.3% (95% CI 14.1% to 25.4%) in
low-risk and high-risk procedures, respectively. For high-risk
procedures, the incidence of intraprocedural bleeding was similar in
patients who interrupted anticoagulation according to BSG/ESGE
guidelines or earlier (10.3%vs10.8%, p=0.99), with a trend for a lower
risk as compared with those who stopped anticoagulation later
(10.3%vs25%, p=0.07). The incidence of delayed bleeding appeared similar
in patients who resumed anticoagulation according to BSG/ESGE guidelines
or later (6.6%vs7.7%, p=0.76), but it tended to increase when DOAC was
resumed earlier (14.4%vs6.6%, p=0.27). The risk of delayed major
bleeding was significantly higher in patients receiving heparin bridging
than in non-bridged ones (26.6%vs5.9%, p=0.017).

Conclusion

High-risk procedures in patients on DOACs are associated with a
substantial risk of bleeding, further increased by heparin bridging.
Adoption of the BSG/ESGE guidelines in periendoscopic management of
DOACs seems to result in a favourable benefit/risk ratio.

Trial registration number

NCT
02734316; Pre-results.
Robust analyses for radiographic progression in rheumatoid arthritis
Authors: Landewé Robert , Sun Luna , Chen Yun-Fei , Daojun Mo , van der Heijde Desirée
Published: April 2023

Demonstrating inhibition of the structural damage to joints as a statistically significant difference in radiographic progression as measured by the van der Heijde modified Total Sharp Score (mTSS) is a common objective in trials for rheumatoid arthritis treatments. The frequently used analysis of the covariance model with missing data imputed using linear extrapolation (analyses of covariance, ANCOVA+LE) may not be ideal for long-term extension studies or for paediatric studies. The random coefficient (RC) model may represent a better alternative.

A two-arm (active treatment and placebo) setting with a week 44 study period was considered. RC model, ANCOVA+LE and ANCOVA with last observation carried forward imputation were compared under different scenarios in bias, root mean square error (RMSE), power and type I error rate.

The RC model outperformed ANCOVA+LE in metrics measuring bias, RMSE, power and type I error rate under the evaluated scenarios. ANCOVA and RC provide similar performance when there are no missing data. With missing data, RC+observed (OBS) provides similar or better results than ANCOVA+LE in power and bias.

Our simulations support that RC is both a more sensitive and a more precise alternative to the commonly used ANCOVA+LE as a primary method for analysing mTSS in long-term extension and paediatric studies with a higher likelihood of missing data. The RC model can provide a reference at time points with missing data by estimating a slope; mTSS change by one unit change in time. ANCOVA+LE is recommended as a sensitivity analysis.
Post-fire impact behaviour of rubberized alkali-activated slag concrete
Authors: Abubakr Ahmed , Soliman Ahmed , Diab Sameh Hassan
Published: April 2023

Rubberized concrete is eco-friendly concrete with improved impact resistance. However, impact loads may also be combined with fire due to blasting, explosions, and earthquake. Hence, this study investigates the post-fire impact behaviour for rubberized alkali-activated slag (AAS) concrete. Static and dynamic properties of AAS mixtures, incorporating 5%, 10%, and 15% crumb rubber, were evaluated under ambient and after exposure to elevated temperatures (200, 400, and 600 °C). Results reveal that AAS properties' degradations were similar to ordinary Portland cement (OPC) at ambient temperature. At elevated temperatures, the differences in hydration products’ nature between AAS and OPC alternated the performance. The formed low Ca/Si ratio calcium silicate hydrated in the AAS exhibited higher stability at elevated temperatures. AAS with 10% rubber content showed an optimum impact performance. Above 400 °C, degradation of the binder microstructure and voids due to rubber particle decomposition adversely affected the impact performance of AAS. The addition of 0.5% steel tire wire showed a high potential sustainable solution to maintain adequate performance.
Care Workers and Managers’ Experiences of Implementing Infection Control Guidance in an Epidemic Context: A Qualitative Study in the South East of England, during the COVID-19 Prevaccination Era
Authors: Bertini Lavinia , Bogen-Johnston Leanne , Sadhwani Shanu , Middleton Jo , Sharp Rebecca , Wood Wendy , Roland Daniel , Forder Julien , Cassell Jackie A.
Published: April 2023

The national response to COVID-19 has had a severe impact on adult social care settings, with high mortality amongst people receiving and providing care in England. Care workers had to rapidly adapt to new infection control measures to protect themselves, their colleagues, and the people receiving care. Infection control in residential and domiciliary care is always complex, but COVID-19 infection control measures impacted exceptionally on care workers’ working and everyday lives. We undertook qualitative interviews with care workers and managers (n = 10) in residential and domiciliary care for older people in the Southeast England during the first wave of the pandemic to understand their experiences, solutions, and concerns to implement guidance in practice. Data were analysed using framework analysis, and the following eight themes were identified: (1) Increasing visibility and support for the sector; (2) the impact of negative messaging about the sector; (3) feelings of isolation; (4) accessibility and usability of guidance; (5) social care staff as agents in producing and sharing good practice; (6) managing expectations and the impact of conflicting messages in the media; (7) improving communication with hospitals; and (8) problems in the early pandemic. The findings reveal widespread concerns for the marginalisation of the sector in the policy response and the inadequacy of infection control guidance. Guidance would benefit from a better understanding of domiciliary and residential care settings. This might involve the following steps: (a) coproduction of guidance with adult social care stakeholders, including those in direct-care roles and (b) a shift away from a clinical model of infection control towards a more flexible approach that attends to the inherent variability of care settings.
Inhibition of Autophagy Maintains ESC Pluripotency and Inhibits Primordial Germ Cell Formation in Chickens
Authors: Ding Ying , Zhao Juanjuan , Xu Xianshuai , Zuo Qisheng , Zhang Yani , Jin Kai , Han Wei , Li Bichun
Published: April 2023

Autophagy plays an important role in the pluripotency and differentiation of stem cells. Transcriptome data showed that the autophagy genes MAP1LC3A and MAP1LC3B were significantly upregulated in primordial germ cells (PGCs). The Kyoto Encyclopedia of Genes and Genome (KEGG) results showed that the lysosome signaling pathway, which is related to autophagy, was significantly enriched in PGCs. Quantitative RT-PCR, western blotting, and transmission electron microscopy (TEM) results showed that autophagy was expressed in both embryonic stem cells (ESCs) and PGCs but was significantly activated in PGCs. To explore the role of autophagy in the differentiation of chicken ESCs into PGCs, autophagy was activated and inhibited using rapamycin and bafilomycin A1, respectively. Results of qRT-PCR, flow cytometry, and indirect immunofluorescence showed that the efficiency of PGC formation significantly decreased after autophagy inhibition. Our results showed, for the first time, that autophagy plays an indispensable role in the formation of chicken PGCs, which lays the foundation for studying the mechanism of autophagy in chicken PGCs and in bird gene editing and the rescue of endangered birds.
Supplementary Dataset 1 from SWI/SNF Blockade Disrupts PU.1-Directed Enhancer Programs in Normal Hematopoietic Cells and Acute Myeloid Leukemia
Authors: Chambers Courtney , Cermakova Katerina , Chan Yuen San , Kurtz Kristen , Wohlan Katharina , Lewis Andrew Henry , Wang Christiana , Pham Anh , Dejmek Milan , Sala Michal , Loeza Cabrera Mario , Aguilar Rogelio , Nencka Radim , Lacorazza H. Daniel , Rau Rachel E. , Hodges H. Courtney
Published: April 2023

RNA-seq datasets
Table ST7 from ROS Induction Targets Persister Cancer Cells with Low Metabolic Activity in NRAS-Mutated Melanoma
Authors: Eichhoff Ossia M. , Stoffel Corinne I. , Käsler Jan , Briker Luzia , Turko Patrick , Karsai Gergely , Zila Nina , Paulitschke Verena , Cheng Phil F. , Leitner Alexander , Bileck Andrea , Zamboni Nicola , Irmisch Anja , Balazs Zsolt , Tastanova Aizhan , Pascoal Susana , Johansen Pål , Wegmann Rebekka , Mena Julien , Othman Alaa , Viswanathan Vasanthi S. , Wenzina Judith , Aloia Andrea , Saltari Annalisa , Dzung Andreas , Krauthammer Michael , Schreiber Stuart L. , Hornemann Thorsten , Distel Martin , Snijder Berend , Dummer Reinhard , Levesque Mitchell P.
Published: April 2023

Table ST7: Clinical data of the 62 melanoma patient which tumor material was used for pharmacoscopy
Table ST6 from ROS Induction Targets Persister Cancer Cells with Low Metabolic Activity in NRAS-Mutated Melanoma
Authors: Eichhoff Ossia M. , Stoffel Corinne I. , Käsler Jan , Briker Luzia , Turko Patrick , Karsai Gergely , Zila Nina , Paulitschke Verena , Cheng Phil F. , Leitner Alexander , Bileck Andrea , Zamboni Nicola , Irmisch Anja , Balazs Zsolt , Tastanova Aizhan , Pascoal Susana , Johansen Pål , Wegmann Rebekka , Mena Julien , Othman Alaa , Viswanathan Vasanthi S. , Wenzina Judith , Aloia Andrea , Saltari Annalisa , Dzung Andreas , Krauthammer Michael , Schreiber Stuart L. , Hornemann Thorsten , Distel Martin , Snijder Berend , Dummer Reinhard , Levesque Mitchell P.
Published: April 2023

Table ST6: Differential expressed lipids and their FDRs in sensitive vs resistant cells (negative values are upregulated in sensitive cells).
Suppl. Figure S6 from ROS Induction Targets Persister Cancer Cells with Low Metabolic Activity in NRAS-Mutated Melanoma
Authors: Eichhoff Ossia M. , Stoffel Corinne I. , Käsler Jan , Briker Luzia , Turko Patrick , Karsai Gergely , Zila Nina , Paulitschke Verena , Cheng Phil F. , Leitner Alexander , Bileck Andrea , Zamboni Nicola , Irmisch Anja , Balazs Zsolt , Tastanova Aizhan , Pascoal Susana , Johansen Pål , Wegmann Rebekka , Mena Julien , Othman Alaa , Viswanathan Vasanthi S. , Wenzina Judith , Aloia Andrea , Saltari Annalisa , Dzung Andreas , Krauthammer Michael , Schreiber Stuart L. , Hornemann Thorsten , Distel Martin , Snijder Berend , Dummer Reinhard , Levesque Mitchell P.
Published: April 2023

S6: Correlation analysis between MEKi and ROS.
Suppl. Figure S5 from ROS Induction Targets Persister Cancer Cells with Low Metabolic Activity in NRAS-Mutated Melanoma
Authors: Eichhoff Ossia M. , Stoffel Corinne I. , Käsler Jan , Briker Luzia , Turko Patrick , Karsai Gergely , Zila Nina , Paulitschke Verena , Cheng Phil F. , Leitner Alexander , Bileck Andrea , Zamboni Nicola , Irmisch Anja , Balazs Zsolt , Tastanova Aizhan , Pascoal Susana , Johansen Pål , Wegmann Rebekka , Mena Julien , Othman Alaa , Viswanathan Vasanthi S. , Wenzina Judith , Aloia Andrea , Saltari Annalisa , Dzung Andreas , Krauthammer Michael , Schreiber Stuart L. , Hornemann Thorsten , Distel Martin , Snijder Berend , Dummer Reinhard , Levesque Mitchell P.
Published: April 2023

S5: Elevated cytoplasmic ROS induces apoptosis after the treatment with neocuproine
Suppl. Figure S15 from ROS Induction Targets Persister Cancer Cells with Low Metabolic Activity in NRAS-Mutated Melanoma
Authors: Eichhoff Ossia M. , Stoffel Corinne I. , Käsler Jan , Briker Luzia , Turko Patrick , Karsai Gergely , Zila Nina , Paulitschke Verena , Cheng Phil F. , Leitner Alexander , Bileck Andrea , Zamboni Nicola , Irmisch Anja , Balazs Zsolt , Tastanova Aizhan , Pascoal Susana , Johansen Pål , Wegmann Rebekka , Mena Julien , Othman Alaa , Viswanathan Vasanthi S. , Wenzina Judith , Aloia Andrea , Saltari Annalisa , Dzung Andreas , Krauthammer Michael , Schreiber Stuart L. , Hornemann Thorsten , Distel Martin , Snijder Berend , Dummer Reinhard , Levesque Mitchell P.
Published: April 2023

S15: Tumor response curves in mice for ionophoric copper-chelators in combination with binimetinib.
Supplemental Methods from A Systematic Review and Multilevel Regression Analysis Reveals the Comorbidity Prevalence in Cancer
Authors: Vrinzen Cilla E.J. , Delfgou Linn , Stadhouders Niek , Hermens Rosella P.M.G. , Merkx Matthias A.W. , Bloemendal Haiko J. , Jeurissen Patrick P.T.
Published: April 2023

Search strings for the literature search and the quality assesment tools
Supplementary Data from HBx-Induced HSPA8 Stimulates HBV Replication and Suppresses Ferroptosis to Support Liver Cancer Progression
Authors: Wang Yufei , Zhao Man , Zhao Lina , Geng Yu , Li Guanghao , Chen Lin , Yu Jingxuan , Yuan Hongfeng , Zhang Huihui , Yun Haolin , Yuan Ying , Wang Guowen , Feng Jinyan , Xu Liang , Wang Shuai , Hou Chunyu , Yang Guang , Zhang Ningning , Lu Wei , Zhang Xiaodong
Published: April 2023

Supplementary materials and methods, Supplementary figures, Supplementary figure legends, Supplementary tables
Supplementary Table S3 from PGC1α Degradation Suppresses Mitochondrial Biogenesis to Confer Radiation Resistance in Glioma
Authors: Zhao Mengjie , Li Yanhui , Lu Chenfei , Ding Fangshu , Xu Miao , Ge Xin , Li Mengdie , Wang Zhen , Yin Jianxing , Zhang Junxia , Wang Xiefeng , Ge Zehe , Xiao Hong , Xiao Yong , Liu Hongyi , Liu Wentao , Cao Yuandong , Wang Qianghu , You Yongping , Wang Xiuxing , Yang Kun , Shi Zhumei , Qian Xu
Published: April 2023

Table S3. Clinical information of patients
Table S1 from TLE3 Sustains Luminal Breast Cancer Lineage Fidelity to Suppress Metastasis
Authors: Anstine Lindsey J. , Majmudar Parth R. , Aponte Amy , Singh Salendra , Zhao Ran , Weber-Bonk Kristen L. , Abdul-Karim Fadi W. , Valentine Mitchell , Seachrist Darcie D. , Grennel-Nickelson Katelyn E. , Cuellar-Vite Leslie , Sizemore Gina M. , Sizemore Steven T. , Webb Bryan M. , Thompson Cheryl L. , Keri Ruth A.
Published: April 2023

Table S1
S8 Legend from TLE3 Sustains Luminal Breast Cancer Lineage Fidelity to Suppress Metastasis
Authors: Anstine Lindsey J. , Majmudar Parth R. , Aponte Amy , Singh Salendra , Zhao Ran , Weber-Bonk Kristen L. , Abdul-Karim Fadi W. , Valentine Mitchell , Seachrist Darcie D. , Grennel-Nickelson Katelyn E. , Cuellar-Vite Leslie , Sizemore Gina M. , Sizemore Steven T. , Webb Bryan M. , Thompson Cheryl L. , Keri Ruth A.
Published: April 2023

Supplemental Figure 8 Legend
S7 from TLE3 Sustains Luminal Breast Cancer Lineage Fidelity to Suppress Metastasis
Authors: Anstine Lindsey J. , Majmudar Parth R. , Aponte Amy , Singh Salendra , Zhao Ran , Weber-Bonk Kristen L. , Abdul-Karim Fadi W. , Valentine Mitchell , Seachrist Darcie D. , Grennel-Nickelson Katelyn E. , Cuellar-Vite Leslie , Sizemore Gina M. , Sizemore Steven T. , Webb Bryan M. , Thompson Cheryl L. , Keri Ruth A.
Published: April 2023

Supplemental Figure 7
S7 Legend from TLE3 Sustains Luminal Breast Cancer Lineage Fidelity to Suppress Metastasis
Authors: Anstine Lindsey J. , Majmudar Parth R. , Aponte Amy , Singh Salendra , Zhao Ran , Weber-Bonk Kristen L. , Abdul-Karim Fadi W. , Valentine Mitchell , Seachrist Darcie D. , Grennel-Nickelson Katelyn E. , Cuellar-Vite Leslie , Sizemore Gina M. , Sizemore Steven T. , Webb Bryan M. , Thompson Cheryl L. , Keri Ruth A.
Published: April 2023

Supplemental Figure 7 Legend
S4 from TLE3 Sustains Luminal Breast Cancer Lineage Fidelity to Suppress Metastasis
Authors: Anstine Lindsey J. , Majmudar Parth R. , Aponte Amy , Singh Salendra , Zhao Ran , Weber-Bonk Kristen L. , Abdul-Karim Fadi W. , Valentine Mitchell , Seachrist Darcie D. , Grennel-Nickelson Katelyn E. , Cuellar-Vite Leslie , Sizemore Gina M. , Sizemore Steven T. , Webb Bryan M. , Thompson Cheryl L. , Keri Ruth A.
Published: April 2023

Supplemental Figure 4
S2 Legend from TLE3 Sustains Luminal Breast Cancer Lineage Fidelity to Suppress Metastasis
Authors: Anstine Lindsey J. , Majmudar Parth R. , Aponte Amy , Singh Salendra , Zhao Ran , Weber-Bonk Kristen L. , Abdul-Karim Fadi W. , Valentine Mitchell , Seachrist Darcie D. , Grennel-Nickelson Katelyn E. , Cuellar-Vite Leslie , Sizemore Gina M. , Sizemore Steven T. , Webb Bryan M. , Thompson Cheryl L. , Keri Ruth A.
Published: April 2023

Supplemental Figure 2 Legend
S1 Legend from TLE3 Sustains Luminal Breast Cancer Lineage Fidelity to Suppress Metastasis
Authors: Anstine Lindsey J. , Majmudar Parth R. , Aponte Amy , Singh Salendra , Zhao Ran , Weber-Bonk Kristen L. , Abdul-Karim Fadi W. , Valentine Mitchell , Seachrist Darcie D. , Grennel-Nickelson Katelyn E. , Cuellar-Vite Leslie , Sizemore Gina M. , Sizemore Steven T. , Webb Bryan M. , Thompson Cheryl L. , Keri Ruth A.
Published: April 2023

Supplemental Figure 1 Legend
REAGENT or RESOURCE from USP48 Stabilizes Gasdermin E to Promote Pyroptosis in Cancer
Authors: Ren Yidan , Feng Maoxiao , Hao Xiaodong , Liu Xiaoyan , Li Juan , Li Peilong , Gao Jie , Qi Qiuchen , Du Lutao , Wang Chuanxin , Wang Qin , Wang Yunshan
Published: April 2023

Reagent or Resource
Figure S5C from Linking Physical Activity to Breast Cancer via Inflammation, Part 2: The Effect of Inflammation on Breast Cancer Risk
Authors: Lou Makayla W.C. , Drummond Ann E. , Swain Christopher T.V. , Milne Roger L. , English Dallas R. , Brown Kristy A. , van Roekel Eline H. , Skinner Tina L. , Moore Melissa M. , Gaunt Tom R. , Martin Richard M. , Lewis Sarah J. , Lynch Brigid M.
Published: April 2023

Supplementary Figure 5C presents dose-response curves for adiponectin and breast cancer risk, by menopausal status
Figure S5B from Linking Physical Activity to Breast Cancer via Inflammation, Part 2: The Effect of Inflammation on Breast Cancer Risk
Authors: Lou Makayla W.C. , Drummond Ann E. , Swain Christopher T.V. , Milne Roger L. , English Dallas R. , Brown Kristy A. , van Roekel Eline H. , Skinner Tina L. , Moore Melissa M. , Gaunt Tom R. , Martin Richard M. , Lewis Sarah J. , Lynch Brigid M.
Published: April 2023

Supplementary Figure 5B presents forest plots for adiponectin and breast cancer risk, excluding studies where exogenous hormone use status was unknown
Table S1 from High-Throughput Peptide Arrays Identify Potential Diagnostic Autoantibody Signatures in Early-Stage Lung Adenocarcinoma
Authors: Luo Rongrong , Zhong Pei , Li Xiying , Cai Juan , Tao Yimin , Xiong Bangzhu , Zheng Hancheng , Zhang Zhishang , Tang Le , Yao Jiarui , Li Yingrui , Shi Yuankai , Han Xiaohong
Published: April 2023

Corresponding abbreviated number for differential peptides probes in correlation plot.
Figure S5 from High-Throughput Peptide Arrays Identify Potential Diagnostic Autoantibody Signatures in Early-Stage Lung Adenocarcinoma
Authors: Luo Rongrong , Zhong Pei , Li Xiying , Cai Juan , Tao Yimin , Xiong Bangzhu , Zheng Hancheng , Zhang Zhishang , Tang Le , Yao Jiarui , Li Yingrui , Shi Yuankai , Han Xiaohong
Published: April 2023

Figure S5. Correlation between differential autoantibody signatures from Early-LUAD versus BLD and blood routine test. Some of the 143 differential autoantibody signatures positively correlated with RDW.CV.
Figure S2 from High-Throughput Peptide Arrays Identify Potential Diagnostic Autoantibody Signatures in Early-Stage Lung Adenocarcinoma
Authors: Luo Rongrong , Zhong Pei , Li Xiying , Cai Juan , Tao Yimin , Xiong Bangzhu , Zheng Hancheng , Zhang Zhishang , Tang Le , Yao Jiarui , Li Yingrui , Shi Yuankai , Han Xiaohong
Published: April 2023

Corresponding signal of previous reported autoantibodies for early lung cancer detection were evaluated in our study cohort with both HuProtTM protein microarray (detail data not shown) and peptide array. All peptides were aligned to 10 proteins using blastp method, 246 peptides were mapped under the set of e-value of 1 and identity of 90. Accumulated peptide signal was set as autoantibody signal of corresponding antigen protein. Nine autoantibodies had effective testing value in HuprotTM. a.t test P value of autoantibodies signals for Early-LUAD versus NHC from peptide array and HuProtTM detection respectively. Two autoantibodies from peptide array and 2 autoantibodies from HuProtTM showed higher signal in Early-LUAD group under P <0.05. Five autoantibodies from peptide array and 2 autoantibodies from HuProtTM showed higher signal in Early-LUAD group under P < 0.1.b.Volcano plot of peptides signals corresponding to 10 autoantigens. X-axis represents the log10 of the fold change, y-axis represents -log10 (P adjust) for Early-LUAD versus NHC. Significantly up-regulated and down-regulated peptides in Early-LUAD group are shown in light red and blue. Two hundred and forty-six peptides mapped to the 10 autoantibodies were shown in green, and 8 significantly upregulated peptides in Early-LUAD shown in green cross. No intersection between the green mapped signals and the peptides used for classifier model (dark red).
Table S6D from Linking Physical Activity to Breast Cancer Risk via Inflammation, Part 1: The Effect of Physical Activity on Inflammation
Authors: Swain Christopher T.V. , Drummond Ann E. , Milne Roger L. , English Dallas R. , Brown Kristy A. , Lou Makayla W.C. , Boing Leonessa , Bageley Amy , Skinner Tina L. , van Roekel Eline H. , Moore Melissa M. , Gaunt Tom R. , Martin Richard M. , Lewis Sarah J. , Lynch Brigid M.
Published: April 2023

Supplementary Table 6D presents findings of the prospective cohort study
Table S2D from Linking Physical Activity to Breast Cancer Risk via Inflammation, Part 1: The Effect of Physical Activity on Inflammation
Authors: Swain Christopher T.V. , Drummond Ann E. , Milne Roger L. , English Dallas R. , Brown Kristy A. , Lou Makayla W.C. , Boing Leonessa , Bageley Amy , Skinner Tina L. , van Roekel Eline H. , Moore Melissa M. , Gaunt Tom R. , Martin Richard M. , Lewis Sarah J. , Lynch Brigid M.
Published: April 2023

Supplementary Table 2D presents the study characteristics of the prospective cohort study
Figure S3A from Linking Physical Activity to Breast Cancer Risk via Inflammation, Part 1: The Effect of Physical Activity on Inflammation
Authors: Swain Christopher T.V. , Drummond Ann E. , Milne Roger L. , English Dallas R. , Brown Kristy A. , Lou Makayla W.C. , Boing Leonessa , Bageley Amy , Skinner Tina L. , van Roekel Eline H. , Moore Melissa M. , Gaunt Tom R. , Martin Richard M. , Lewis Sarah J. , Lynch Brigid M.
Published: April 2023

Supplementary Figure 3A presents forest plots for physical activity and IL-6, by menopausal status
Suppl Fig 1 from Targeting the Inducible T-cell Costimulator (ICOS) in Patients with Relapsed/Refractory T-follicular Helper Phenotype Peripheral T-cell and Angioimmunoblastic T-cell Lymphoma
Authors: Chavez Julio C. , Foss Francine M. , William Basem M. , Brammer Jonathan E. , Smith Sonali M. , Prica Anca , Zain Jasmine M. , Tuscano Joseph M. , Shah Harsh , Mehta-Shah Neha , Geethakumari Praveen Ramakrishnan , Wang Ben X. , Zantinge Stephanie , Wang Lisa , Zhang Ling , Boutrin Anmarie , Zhao Weiguang , Cheng Lily , Standifer Nathan , Hewitt Lisa , Enowtambong Enowmpey , Shao Weiping , Sharma Shringi , Carlesso Gianluca , Moscow Jeffrey A. , Siu Lillian L.
Published: April 2023

Supplemental Figure 1. CD4 count recovery after end of treatment (by local site collection)
Supplementary Data1 from Tumor Mutational Burden as a Predictor of Survival with Durvalumab and/or Tremelimumab Treatment in Recurrent or Metastatic Head and Neck Squamous Cell Carcinoma
Authors: Wildsmith Sophie , Li Weimin , Wu Song , Stewart Ross , Morsli Nassim , Raja Rajiv , Zhang Qu , Ye Jiabu , He Philip , Shetty Jagdish , Yovine Alejandro , Holoweckyj Nicholas , Real Katia , Walker Jill , Wrona Magdalena , de los Reyes Melissa , Barker Craig , Whiteley Jessica , Haddad Robert , Licitra Lisa , Ferris Robert , Fayette Jérôme , Zandberg Dan P. , Siu Lillian L. , Mesía Ricard
Published: April 2023

Supplementary Data
Figure S2 from Tumor Mutational Burden as a Predictor of Survival with Durvalumab and/or Tremelimumab Treatment in Recurrent or Metastatic Head and Neck Squamous Cell Carcinoma
Authors: Wildsmith Sophie , Li Weimin , Wu Song , Stewart Ross , Morsli Nassim , Raja Rajiv , Zhang Qu , Ye Jiabu , He Philip , Shetty Jagdish , Yovine Alejandro , Holoweckyj Nicholas , Real Katia , Walker Jill , Wrona Magdalena , de los Reyes Melissa , Barker Craig , Whiteley Jessica , Haddad Robert , Licitra Lisa , Ferris Robert , Fayette Jérôme , Zandberg Dan P. , Siu Lillian L. , Mesía Ricard
Published: April 2023

FIG S2. Kaplan-Meier plot of OS in the durvalumab monotherapy, durvalumab plus tremelimumab and SoC treatment arms in the NLR ≤ 7 subgroup (A) and NLR > 7 subgroup (B) in EAGLE.
Figure S1 from Tumor Mutational Burden as a Predictor of Survival with Durvalumab and/or Tremelimumab Treatment in Recurrent or Metastatic Head and Neck Squamous Cell Carcinoma
Authors: Wildsmith Sophie , Li Weimin , Wu Song , Stewart Ross , Morsli Nassim , Raja Rajiv , Zhang Qu , Ye Jiabu , He Philip , Shetty Jagdish , Yovine Alejandro , Holoweckyj Nicholas , Real Katia , Walker Jill , Wrona Magdalena , de los Reyes Melissa , Barker Craig , Whiteley Jessica , Haddad Robert , Licitra Lisa , Ferris Robert , Fayette Jérôme , Zandberg Dan P. , Siu Lillian L. , Mesía Ricard
Published: April 2023

FIG S1. TMB distribution in samples from HAWK and CONDOR (A) and EAGLE (B).
Supplementary Figures 1-8 from ATM and p53 Regulate FOXM1 Expression via E2F in Breast Cancer Epirubicin Treatment and Resistance
Authors: Millour Julie , de Olano Natalia , Horimoto Yoshiya , Monteiro Lara J. , Langer Julia K. , Aligue Rosa , Hajji Nabil , Lam Eric W.-F.
Published: April 2023

Supplementary Figures 1-8 from ATM and p53 Regulate FOXM1 Expression via E2F in Breast Cancer Epirubicin Treatment and Resistance
Supplementary Figure Legend from Predominance of mTORC1 over mTORC2 in the Regulation of Proliferation of Ovarian Cancer Cells: Therapeutic Implications
Authors: Montero Juan Carlos , Chen Xi , Ocaña Alberto , Pandiella Atanasio
Published: April 2023

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Supplementary Figure 2 from Inhibition of Dynamin by Dynole 34-2 Induces Cell Death following Cytokinesis Failure in Cancer Cells
Authors: Chircop Megan , Perera Swetha , Mariana Anna , Lau Hui , Ma Maggie P.C. , Gilbert Jayne , Jones Nigel C. , Gordon Christopher P. , Young Kelly A. , Morokoff Andrew , Sakoff Jennette , O'Brien Terence J. , McCluskey Adam , Robinson Phillip J.
Published: April 2023

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