bluuebunny/crossref_metadata_2025 huggingface dataset:
Cluster Analysis of Short Sensory Profile Data Reveals Sensory-Based Subgroups in Autism Spectrum Disorder
Authors: Lyons-Warren Ariel M. , Wangler Michael F. , Wan Ying-Wooi
Published: October 2022

Autism spectrum disorder is a common, heterogeneous neurodevelopmental disorder lacking targeted treatments. Additional features include restricted, repetitive patterns of behaviors and differences in sensory processing. We hypothesized that detailed sensory features including modality specific hyper- and hypo-sensitivity could be used to identify clinically recognizable subgroups with unique underlying gene variants. Participants included 378 individuals with a clinical diagnosis of autism spectrum disorder who contributed Short Sensory Profile data assessing the frequency of sensory behaviors and whole genome sequencing results to the Autism Speaks’ MSSNG database. Sensory phenotypes in this cohort were not randomly distributed with 10 patterns describing 43% (162/378) of participants. Cross comparison of two independent cluster analyses on sensory responses identified six distinct sensory-based subgroups. We then characterized subgroups by calculating the percent of patients in each subgroup who had variants with a Combined Annotation Dependent Depletion (CADD) score of 15 or greater in each of 24,896 genes. Each subgroup exhibited a unique pattern of genes with a high frequency of variants. These results support the use of sensory features to identify autism spectrum disorder subgroups with shared genetic variants.
Lung Inflammasome Activation in SARS-CoV-2 Post-Mortem Biopsies
Authors: Baena Carstens Lucas , Campos D’amico Raissa , Fernandes de Moura Karen , Morais de Castro Eduardo , Centenaro Flávia , Silva Barbosa Giovanna , Vieira Cavalcante da Silva Guilherme , Brenny Isadora , Honório D’Agostini Júlio César , Hlatchuk Elisa Carolina , Pissette de Lima Sabrina , Camargo Martins Ana Paula , De Castro Deus Marina , Konzen Klein Carolline , Kubaski Benevides Ana Paula , Nagashima Seigo , Machado-Souza Cleber , Pinho Ricardo A , Pellegrino Baena Cristina , de Noronha Lúcia
Published: October 2022

The inflammasome complex is a key part of chronic diseases and acute infections, being responsible for cytokine release and cell death mechanism regulation. The SARS-CoV-2 infection is characterized by a dysregulated cytokine release. In this context, the inflammasome complex analysis within SARS-CoV-2 infection may prove beneficial to understand the disease’s mechanisms. Post-mortem minimally invasive autopsies were performed in patients who died from COVID-19 (n = 24), and lung samples were compared to a patient control group (n = 11) and an Influenza A virus H1N1 subtype group from the 2009 pandemics (n = 10). Histological analysis was performed using hematoxylin-eosin staining. Immunohistochemical (IHC) staining was performed using monoclonal antibodies against targets: ACE2, TLR4, NF-κB, NLRP-3 (or NALP), IL-1β, IL-18, ASC, CASP1, CASP9, GSDMD, NOX4, TNF-α. Data obtained from digital analysis underwent appropriate statistical tests. IHC analysis showed biomarkers that indicate inflammasome activation (ACE2; NF-κB; NOX4; ASC) were significantly increased in the COVID-19 group (p < 0.05 for all) and biomarkers that indicate cell pyroptosis and inflammasome derived cytokines such as IL-18 (p < 0.005) and CASP1 were greatly increased (p < 0.0001) even when compared to the H1N1 group. We propose that the SARS-CoV-2 pathogenesis is connected to the inflammasome complex activation. Further studies are still warranted to elucidate the pathophysiology of the disease.
Genome-Wide Identification and Characterization of YUCCA Gene Family in Mikania micrantha
Authors: Luo Weigui , Xiao Nian , Wu Feiyan , Mo Beixin , Kong Wenwen , Yu Yu
Published: October 2022

Auxin is a general coordinator for growth and development throughout plant lifespan, acting in a concentration-dependent manner. Tryptophan aminotransferases (YUCCA) family catalyze the oxidative decarboxylation of indole-3-pyruvic acid (IPA) to form indole-3-acetic acid (IAA) and plays a critical role in auxin homeostasis. Here, 18 YUCCA family genes divided into four categories were identified from Mikania micrantha (M. micrantha), one of the world’s most invasive plants. Five highly conserved motifs were characterized in these YUCCA genes (MmYUCs). Transcriptome analysis revealed that MmYUCs exhibited distinct expression patterns in different organs and five MmYUCs showed high expression levels throughout all the five tissues, implying that they may play dominant roles in auxin biosynthesis and plant development. In addition, MmYUC6_1 was overexpressed in DR5::GUS Arabidopsis line to explore its function, which resulted in remarkably increased auxin level and typical elevated auxin-related phenotypes including shortened roots and elongated hypocotyls in the transgenic plants, suggesting that MmYUC6_1 promoted IAA biosynthesis in Arabidopsis. Collectively, these findings provided comprehensive insight into the phylogenetic relationships, chromosomal distributions, expression patterns and functions of the MmYUC genes in M. micrantha, which would facilitate the study of molecular mechanisms underlying the fast growth of M. micrantha and preventing its invasion.
The Roles of Imaging Biomarkers in the Management of Chronic Neuropathic Pain
Authors: Pricope Cosmin Vasilica , Tamba Bogdan Ionel , Stanciu Gabriela Dumitrita , Cuciureanu Magdalena , Neagu Anca Narcisa , Creanga-Murariu Ioana , Dobrovat Bogdan-Ionut , Uritu Cristina Mariana , Filipiuc Silviu Iulian , Pricope Bianca-Mariana , Alexa-Stratulat Teodora
Published: October 2022

Chronic neuropathic pain (CNP) affects around 10% of the general population and has a significant social, emotional, and economic impact. Current diagnosis techniques rely mainly on patient-reported outcomes and symptoms, which leads to significant diagnostic heterogeneity and subsequent challenges in management and assessment of outcomes. As such, it is necessary to review the approach to a pathology that occurs so frequently, with such burdensome and complex implications. Recent research has shown that imaging methods can detect subtle neuroplastic changes in the central and peripheral nervous system, which can be correlated with neuropathic symptoms and may serve as potential markers. The aim of this paper is to review available imaging methods used for diagnosing and assessing therapeutic efficacy in CNP for both the preclinical and clinical setting. Of course, further research is required to standardize and improve detection accuracy, but available data indicate that imaging is a valuable tool that can impact the management of CNP.
Molecular and Cellular Interactions in Pathogenesis of Sporadic Parkinson Disease
Authors: Dolgacheva Lyudmila P. , Zinchenko Valery P. , Goncharov Nikolay V.
Published: October 2022

An increasing number of the population all around the world suffer from age-associated neurodegenerative diseases including Parkinson’s disease (PD). This disorder presents different signs of genetic, epigenetic and environmental origin, and molecular, cellular and intracellular dysfunction. At the molecular level, α-synuclein (αSyn) was identified as the principal molecule constituting the Lewy bodies (LB). The gut microbiota participates in the pathogenesis of PD and may contribute to the loss of dopaminergic neurons through mitochondrial dysfunction. The most important pathogenetic link is an imbalance of Ca2+ ions, which is associated with redox imbalance in the cells and increased generation of reactive oxygen species (ROS). In this review, genetic, epigenetic and environmental factors that cause these disorders and their cause-and-effect relationships are considered. As a constituent of environmental factors, the example of organophosphates (OPs) is also reviewed. The role of endothelial damage in the pathogenesis of PD is discussed, and a ‘triple hit hypothesis’ is proposed as a modification of Braak’s dual hit one. In the absence of effective therapies for neurodegenerative diseases, more and more evidence is emerging about the positive impact of nutritional structure and healthy lifestyle on the state of blood vessels and the risk of developing these diseases.
Linking Nonalcoholic Fatty Liver Disease and Brain Disease: Focusing on Bile Acid Signaling
Authors: Ren Zi-Lin , Li Chang-Xiang , Ma Chong-Yang , Chen Dan , Chen Jia-Hui , Xu Wen-Xiu , Chen Cong-Ai , Cheng Fa-Feng , Wang Xue-Qian
Published: October 2022

A metabolic illness known as non-alcoholic fatty liver disease (NAFLD), affects more than one-quarter of the world’s population. Bile acids (BAs), as detergents involved in lipid digestion, show an abnormal metabolism in patients with NAFLD. However, BAs can affect other organs as well, such as the brain, where it has a neuroprotective effect. According to a series of studies, brain disorders may be extrahepatic manifestations of NAFLD, such as depression, changes to the cerebrovascular system, and worsening cognitive ability. Consequently, we propose that NAFLD affects the development of brain disease, through the bile acid signaling pathway. Through direct or indirect channels, BAs can send messages to the brain. Some BAs may operate directly on the central Farnesoid X receptor (FXR) and the G protein bile acid-activated receptor 1 (GPBAR1) by overcoming the blood–brain barrier (BBB). Furthermore, glucagon-like peptide-1 (GLP-1) and the fibroblast growth factor (FGF) 19 are released from the intestine FXR and GPBAR1 receptors, upon activation, both of which send signals to the brain. Inflammatory, systemic metabolic disorders in the liver and brain are regulated by the bile acid-activated receptors FXR and GPBAR1, which are potential therapeutic targets. From a bile acid viewpoint, we examine the bile acid signaling changes in NAFLD and brain disease. We also recommend the development of dual GPBAR1/FXR ligands to reduce side effects and manage NAFLD and brain disease efficiently.
Granulocytic MDSC with Deficient CCR5 Alleviates Lipogenesis and Inflammation in Nonalcoholic Fatty Liver Disease
Authors: Liao Tzu-Chieh , Huang Jiung-Pang , Tsai Yu-Ting , Shih Wei-Ching , Juan Chi-Chang , Hsieh Po-Shiuan , Hung Li-Man , Yu Chao-Lan
Published: October 2022

C-C chemokine receptor type 5 (CCR5) positively contributes to the pathogenesis of nonalcoholic fatty liver disease (NAFLD), a common metabolic liver disease associated with chronic inflammation. CCR5 signaling also facilitates the immunosuppressive activity of a group of immature myeloid cells known as granulocytic myeloid-derived suppressor cells (g-MDSCs). While both hepatocyte and g-MDSC express CCR5, how CCR5 coordinates these two distinct cell types in the hepatic microenvironment remains largely unknown. Here, we used in vivo and ex vivo approaches to define the molecular details of how CCR5 mediates the crosstalk between hepatocytes and g-MDSCs in a mouse model of NAFLD. Global CCR5-deficient mice exhibited more severe steatosis, increased hepatic gene expression of lipogenesis, and exacerbated liver damage in diet-induced obesity. Either NAFLD or CCR5-deficiency per se is causative for the increase of g-MDSCs. Purified g-MDSCs have a higher survival rate in the fatty liver microenvironment, and blockade of CCR5 significantly decreases g-MDSCs’ expression of anti-inflammatory factors. On the other hand, the null of CCR5 signaling increases hepatocytes’ expression of lipogenic genes in the NAFLD microenvironment. Most importantly, inhibiting g-MDSCs’ CCR5 signaling in the fatty liver microenvironment dramatically reduces STAT3 signaling, lipogenic, and pro-inflammatory gene expression in primary hepatocytes. Adoptive cell transfer experiments further demonstrate that CCR5-deficient g-MDSCs mitigate hepatic lipogenic gene expression without facilitating pro-inflammatory cytokine production and liver damage in NAFLD mice. These results suggest that targeting g-MDSCs’ CCR5 signaling might serve as a potential therapeutic strategy for NAFLD.
Counteracting Immunosuppression in the Tumor Microenvironment by Oncolytic Newcastle Disease Virus and Cellular Immunotherapy
Authors: Schirrmacher Volker , van Gool Stefaan , Stuecker Wilfried
Published: October 2022

An apparent paradox exists between the evidence for spontaneous systemic T cell- mediated anti-tumor immune responses in cancer patients, observed particularly in their bone marrow, and local tumor growth in the periphery. This phenomenon, known as “concomitant immunity” suggests that the local tumor and its tumor microenvironment (TME) prevent systemic antitumor immunity to become effective. Oncolytic Newcastle disease virus (NDV), an agent with inherent anti-neoplastic and immune stimulatory properties, is capable of breaking therapy resistance and immunosuppression. This review updates latest information about immunosuppression by the TME and discusses mechanisms of how oncolytic viruses, in particular NDV, and cellular immunotherapy can counteract the immunosuppressive effect of the TME. With regard to cellular immunotherapy, the review presents pre-clinical studies of post-operative active-specific immunotherapy and of adoptive T cell-mediated therapy in immunocompetent mice. Memory T cell (MTC) transfer in tumor challenged T cell-deficient nu/nu mice demonstrates longevity and functionality of these cells. Graft-versus-leukemia (GvL) studies in mice demonstrate complete remission of late-stage disease including metastases and cachexia. T cell based immunotherapy studies with human cells in human tumor xenotransplanted NOD/SCID mice demonstrate superiority of bone marrow-derived as compared to blood-derived MTCs. Results from clinical studies presented include vaccination studies using two different types of NDV-modified cancer vaccine and a pilot adoptive T-cell mediated therapy study using re-activated bone marrow-derived cancer-reactive MTCs. As an example for what can be expected from clinical immunotherapy against tumors with an immunosuppressive TME, results from vaccination studies are presented from the aggressive brain tumor glioblastoma multiforme. The last decades of basic research in virology, oncology and immunology can be considered as a success story. Based on discoveries of these research areas, translational research and clinical studies have changed the way of treatment of cancer by introducing and including immunotherapy.
Depletion of Arg1-Positive Microglia/Macrophages Exacerbates Cerebral Ischemic Damage by Facilitating the Inflammatory Response
Authors: Li Ting , Zhao Jin , Gao Hao
Published: October 2022

Stroke is a serious worldwide disease that causes death and disability, more than 80% of which is ischemic stroke. The expression of arginase 1 (Arg1), a key player in regulating nitrogen homeostasis, is altered in the peripheral circulation after stroke. Growing evidence indicates that ischemic stroke also induces upregulated Arg1 expression in the central nervous system, especially in activated microglia and macrophages. This implies that Arg1 may affect stroke progression by modulating the cerebral immune response. To investigate the effect of Arg1+ microglia/macrophages on ischemic stroke, we selectively eliminated cerebral Arg1+ microglia/macrophages by mannosylated clodronate liposomes (MCLs) and investigated their effects on behavior, neurological deficits, and inflammatory responses in mice after ischemic stroke. More than half of Arg1+ cells, mainly Arg1+ microglia/macrophages, were depleted after MCLs administration, resulting in a significant deterioration of motility in mice. After the elimination of Arg1+ microglia/macrophages, the infarct volume expanded and neuronal degenerative lesions intensified. Meanwhile, the absence of Arg1+ microglia/macrophages significantly increased the production of pro-inflammatory cytokines and suppressed the expression of anti-inflammatory factors, thus profoundly altering the immune microenvironment at the lesion site. Taken together, our data demonstrate that depletion of Arg1+ microglia/macrophages exacerbates neuronal damage by facilitating the inflammatory response, leading to more severe ischemic injury. These results suggest that Arg1+ microglia/macrophages, as a subpopulation regulating inflammation, is beneficial in controlling the development of ischemia and promoting recovery from injury. Regulation of Arg1 expression on microglia/macrophages at the right time may be a potential target for the treatment of ischemic brain injury.
Atherogenic Indices as a Predictor of Aortic Calcification in Prostate Cancer Patients Assessed Using 18F-Sodium Fluoride PET/CT
Authors: Dai Michelle , Xu Winnie , Chesnais Helene , Anabaraonye Nancy , Parente James , Chatterjee Shampa , Rajapakse Chamith S.
Published: October 2022

A major pathophysiological cause of cardiovascular disease is vascular plaque calcification. Fluorine 18–Sodium Fluoride (18F-NaF) PET/CT can be used as a sensitive imaging modality for detection of vascular calcification. The aim of this study was to find a non-invasive, cost-efficient, and readily available metric for predicting vascular calcification severity. This retrospective study was performed on 36 participants who underwent 18F-NaF fused PET/CT scans. The mean standard uptake values (SUVs) were calculated from manually sectioned axial sections over the aortic arch and thoracic aorta. Correlation analyses were performed between SUVs and calculated atherogenic indices (AIs). Castelli’s Risk Index I (r = 0.63, p < 0.0001), Castelli’s Risk Index II (r = 0.64, p < 0.0001), Atherogenic Coefficient (r = 0.63, p < 0.0001), Atherogenic Index of Plasma (r = 0.51, p = 0.00152), and standalone high-density lipoprotein (HDL) cholesterol (r = −0.53, p = 0.000786) were associated with aortic calcification. AIs show strong association with aortic arch and thoracic aorta calcifications. AIs are better predictors of vascular calcification compared to standalone lipid metrics, with the exception of HDL cholesterol. Clinical application of AIs provides a holistic metric beneficial for enhancing screening and treatment protocols.
Nitric Oxide Metabolic Pathway in Drought-Stressed Nodules of Faba Bean (Vicia faba L.)
Authors: Chammakhi Chaima , Boscari Alexandre , Pacoud Marie , Aubert Grégoire , Mhadhbi Haythem , Brouquisse Renaud
Published: October 2022

Drought is an environmental stress that strongly impacts plants. It affects all stages of growth and induces profound disturbances that influence all cellular functions. Legumes can establish a symbiosis with Rhizobium-type bacteria, whose function is to fix atmospheric nitrogen in organs called nodules and to meet plant nitrogen needs. Symbiotic nitrogen fixation (SNF) is particularly sensitive to drought. We raised the hypothesis that, in drought-stressed nodules, SNF inhibition is partly correlated to hypoxia resulting from nodule structure compaction and an increased O2 diffusion barrier, and that the nodule energy regeneration involves phytoglobin–nitric oxide (Pgb–NO) respiration. To test this hypothesis, we subjected faba bean (Vicia faba L.) plants nodulated with a Rhizobium laguerreae strain to either drought or osmotic stress. We monitored the N2-fixation activity, the energy state (ATP/ADP ratio), the expression of hypoxia marker genes (alcohol dehydrogenase and alanine aminotransferase), and the functioning of the Pgb–NO respiration in the nodules. The collected data confirmed our hypothesis and showed that (1) drought-stressed nodules were subject to more intense hypoxia than control nodules and (2) NO production increased and contributed via Pgb–NO respiration to the maintenance of the energy state of drought-stressed nodules.
In Vivo Assessments of Mesoblastic Nephroma (Ne/De) and Myelomonoblastic Leukaemia (My1/De) Tumour Development in Hypercholesterolemia Rat Models
Authors: Képes Zita , Barkóczi Alexandra , Szabó Judit P. , Kálmán-Szabó Ibolya , Arató Viktória , Garai Ildikó , Árkosy Péter , Jószai István , Deák Ádám , Kertész István , Hajdu István , Trencsényi György
Published: October 2022

Given the rising prevalence of lipid metabolic disorders and malignant diseases, we aimed to establish an in vivo hypercholesterinaemic tumour-bearing rat model for the induction and assessment of these conditions. A normal standard CRLT/N, 2 (baseline),- or 4 (2 + 2, pretreated)-week-long butter and cholesterol rich (BCR) diet was applied to mesoblastic nephroma (Ne/De) and myelomonoblastic leukaemia (My1/De) tumour-bearing and healthy control Long—Evans and Fischer 344 rats. The beginning of chow administration started in parallel with tumour induction and the 2 weeks of pre-transplantation in the baseline and pretreated groups, respectively. Fourteen days post-inoculation, the measurement of lipid parameters and [18F]F-FDG PET/MRI examinations was executed. The comparable lipid status of baseline healthy and tumorous rats proves that regardless of tumour presence, BCR-based hypercholesterolemia was achieved. A higher tumour mass among pretreated tumorous animals was found when compared to the control groups (p < 0.05, p < 0.01). Further, a visually greater [18F]F-FDG accumulation was observed in pretreated BCR tumorous animals; however, the quantitative data (SUVmean: 9.86 ± 0.98, 9.68 ± 1.24; SUVmax: 19.63 ± 1.20; 17.56 ± 3.21 for Ne/De and My1/De, respectively) were not statistically significantly different from those of the CRLT/N tumorous rats (SUVmean: 8.40 ± 1.42, 7.22 ± 1.06 and SUVmax: 15.99 ± 2.22, 12.46 ± 1.96 for control Ne/De and My1/De, respectively). Our model seems to be appropriate for simultaneously investigating hypercholesterolemia and cancer in the same rat.
Solving Decision-Making Problems Using a Measure for Information Values Connected to the Equilibrium Points (IVEP) MCDM Method and Zakeri–Konstantas Performance Correlation Coefficient
Authors: Zakeri Shervin , Konstantas Dimitri
Published: October 2022

In this paper, a new multicriteria decision-making (MCDM) method, called a measure for information values connected to the equilibrium points (IVEP) method, and a new statistical measure for measuring the similarities of performances of MCDM algorithm outputs in a comparison process, called the Zakeri–Konstantas performance correlation coefficient, are introduced. The IVEP method uses Shannon’s entropy as the primary tool to measure the information embedded in the decision matrix in order to evaluate the decision’s options/alternatives for complex decision-making problems with a large number of criteria and alternatives. The second concept that drives the IVEP method is the equilibrium points, which signify the points in a vector space where scores for the decision’s options/alternatives are equilibrated. Instead of using linear functions to compute similarities between the data sets generated by the MCDM algorithms by the calculation of the distance using different methods, the Zakeri–Konstantas performance correlation coefficient focuses on the evaluation of the ranking performance of MCDM methods in an analytic comparison process in order to determine the degree of the similarities. The IVEP method is applied to a real-world decision-making problem—a material selection problem. A comparison analysis was performed on the results obtained from the IVEP, TOPSIS, WPM, COPRAS, and ARAS MCDM methods by the Zakeri–Konstantas performance correlation coefficient and the Hamming distance. The results of both measures revealed that the IVEP algorithm’s outputs have the highest similarity to TOPSIS outputs, among others. Nevertheless, the degree of the similarities is distinct due to the different approaches of the measures used.
Development of a Chatbot for Pregnant Women on a Posyandu Application in Indonesia: From Qualitative Approach to Decision Tree Method
Authors: Puspitasari Indriana Widya , Rinawan Fedri Ruluwedrata , Purnama Wanda Gusdya , Susiarno Hadi , Susanti Ari Indra
Published: October 2022

With the widespread application of digital healthcare, mobile health (mHealth) services are also developing in maternal and child health, primarily through community-based services, such as Posyandu in Indonesia. Patients need media for consultation and decision-making, while health workers are constrained in responding quickly. This study aimed to obtain information from pregnant women and midwives in developing a decision tree model as material for building a semi-automated chatbot. Using an exploratory qualitative approach, semi-structured interviews were conducted through focus group discussions (FGD) with pregnant women (n = 10) and midwives (n = 12) in March 2022. The results showed 38 codes, 15 categories, and 7 subthemes that generated 3 major themes: maternal health education, information on maternal health services, and health monitoring. The decision tree method was applied from these themes based on the needs of users, evidence, and expert sources to ensure quality. In summary, the need to use a semi-automated chatbot can be applied to education about maternal health and monitoring, where severe cases should be provided with non-automated communication with midwives. Applying the decision tree method ensured quality content, supported a clinical decision, and assisted in early detection. Furthermore, future research needs to measure user evaluation.
Advantage of Regional Algorithms for the Chlorophyll-a Concentration Retrieval from In Situ Optical Measurements in the Kara Sea
Authors: Korchemkina Elena , Deryagin Dmitriy , Pavlova Mariia , Kostyleva Anna , Kozlov Igor E. , Vazyulya Svetlana
Published: October 2022

The data of the sea reflectance coefficient were obtained during the cruise of the R/V “Akademik Ioffe” (10 August–8 September 2021) in the Kara Sea beyond the Arctic Circle. A total of 28 measurements of sea reflectance were performed in different conditions ranging from estuarine zones to open ocean. In addition, at 10 stations, water samples were taken, and chlorophyll-a concentrations were determined using the fluorometric method. In situ reflectance data were compared to satellite data (MODIS Aqua/Terra, Sentinel OLCI 3A/B, VIIRS SNPP) obtained within 24 h before and after the measurement. It was shown that, in general, an overestimation of remote sensing reflectance is observed in short-wave channels (412, 443, 469 nm for MODIS; 410, 412.5, 442.5 for OLCI) and underestimation in long-wave channels (708.75, 753.75 nm for OLCI). The obtained in situ chlorophyll-a concentrations were compared with the results of standard models (GIOP, OC2, OC3, OC4), and regional algorithms (semi-analytical MHI, empirical K17) were applied to the in situ and remotely sensed reflectances. In the cases of GIOP and OC4, unrealistic concentrations of chlorophyll-a were obtained. Regional algorithms developed earlier, specifically for the Kara Sea, showed good correlation (0.6 and 0.76) with in situ measurements. Despite the approximately 20% overestimation of chlorophyll-a by both regional algorithms, they can be used to obtain chlorophyll-a concentrations in the Kara Sea in a concentration range of <1 mg/m3.
Broken Ice Photogrammetry in Model-Scale Experiments with Sloped Structure
Authors: Zvyagin Petr , Pavlov Ilia , Zvyagina Tatiana
Published: October 2022

Testing a physical model of an ice-resistant marine structure in an ice tank is one of the methods used for design validation. For a stationary structure design, not only is the possible global ice load of interest but also the processes of creation and evolution of ice rubble in front of the contacting surface. While the load registering technique in model-scale experiments is very well-developed, the photogrammetric analysis of broken ice morphometry and locomotion is not. The photographs taken to illustrate the breaking process do not usually accompany the information necessary for the photogrammetric reconstruction of the scene. This paper outlines a systematic approach to the photogrammetric analysis of the scenes in model-scale conditions. Using this approach, the broken ice dimensions were measured in seven model-scale experiments for which the model of a sloped marine structure was reconstructed. In these experiments, a 700 mm wide slope with an inclination angle of 53° caused an upward flexural failure of the model’s granular ice. Reference global load histories for these experiments are provided. For the first contact episodes, the successful reconstruction of the broken ice mosaic in the polynya showed the insignificant contribution of compressive failure. In continual ice–structure interaction, the morphometry of the ice blocks visible on the slope of the rubble pile and on the surface of the surrounding ice sheet was retrieved from orthorectified video frames. The results were compared with the after-test nadir drone view of the polynya. The error in estimating the top-side area and the maximum linear dimension of the ice block fell into the interval of 0–10%. The morphometric information of the broken ice floes obtained in ice tank experiments with physical models can be used for the improvement of the mechanical models of ice fracture and failure against inclined offshore ice-resistant structures.
Research on the Coordination of Quality Behavior of Supply 3 Chain of E-Commerce Platform under C2B Model of High-Grade E-Commerce Based on Differential Game
Authors: Xu Bin , Zhang Zhouhao , Li Xinqi
Published: October 2022

With the increasing demands of consumers for product grade, the C2B model of high-grade e-commerce emerges as required. In order to solve the problem of coordination and cooperation between e-commerce platforms and manufacturers, and to further develop the C2B model of high-grade e-commerce, this paper studies the coordination of supply chain interests by establishing a differential game model considering product grade factors. By comparing the equilibrium solutions of differential games under different decision-making situations, a cooperative coordination mechanism is proposed. Next, the equilibrium solution is further analyzed by means of numerical simulation. Finally, the influence of several important parameters on the equilibrium solution is discussed through sensitivity analysis. It is found that (1) the supply chain parties have optimal quality behavior in the centralized decision-making situation, and the overall benefit is the greatest. (2) Compared with the Nash non-cooperative game, the optimal quality behavior of the dominant party remains unchanged, and the optimal quality behavior of the following party is enhanced after both parties move from the Stackelberg master-slave game, and the optimal profits of both parties, as well as the overall increase. (3) The cooperative coordination model can coordinate the quality behavior of both sides of the supply chain when the benefit distribution coefficient is within a specific range.
Synthesis and Inhibition of Influenza H1N1 Virus by Indolo-Glycyrrhetic Acid Cyanoesters
Authors: Petrova Anastasiya V. , Belyaevskaya Svetlana V. , Zarubaev Vladimir V.
Published: October 2022

Two new indolo-glycyrrhetic acid derivatives containing cyano-substitutent at C30 have been synthesized, and their anti-influenza activity has been evaluated in vitro. The resulting data demonstrated moderate inhibitory activity against the H1N1 virus with IC50 29 and 23 μM, respectively. The value of SI 18 and 21 confirm low toxicity and potential of new compounds for following research and development of new biological agents.
Effects of Fiber and Surface Treatment on Airport Pavement Concrete against Freeze–Thawing and Salt Freezing
Authors: Xu Lei , Lai Yong , Ma Daoxun , Wang Junjie , Li Molan , Li Le , Gao Zhibin , Liu Yan , He Pukang , Zhang Yi
Published: October 2022

Airport pavement concrete often suffers from freeze–thawing damage in high latitude and cold areas. In addition, the use of aircraft deicer makes the airport pavement concrete suffer from salt-freezing damage. To improve the durability of airport pavement concrete, modified polyester synthetic fiber (FC), cellulose fiber (CF), and basalt fiber (BF) reinforced concrete were prepared in this paper. The mechanical strength, pore structure, and frost resistance (freeze–thawing and salt freezing) of fiber-reinforced concrete were investigated. The effects of the combined action of fiber (fiber type and content) and surface treatment methods (spraying silane and impregnating silane) on the frost resistance of concrete were investigated. The results show that the flexural strength of concrete is positively correlated with the elastic modulus of fiber, but has little effect on the compressive strength. Fiber can reduce mass loss and dynamic modulus loss of concrete subjected to frost damage. FC more effectively improved the frost resistance of concrete than CF. After 30 cycles of salt freezing, the spalling amount of concrete sprayed or soaked with silane was decreased by 65.5% and 55.5%, respectively. Adding fiber and impregnating silane reduced the spalled concrete by up to 70.5%. Spraying silane treatment is better than impregnating silane treatment in enhancing the frost resistance of concrete because a better silane condensation reaction is achieved with spraying silane.
Synthesis of Three-Dimensional Carbon Nanosheets and Its Flux Pinning Mechanisms in C-Doped MgB2 Superconductors
Authors: Zhao Qian , Chen Yun , Qin Baojun , Hu Chunhao , Xia Guoqing , Hao Liang , Ping Xuecheng
Published: October 2022

Three-dimensional carbon nanosheets (3D-CNS) were synthesized by salt template spray-drying method in order to solve the agglomeration of 2D nanocarbon by a traditional mixing method. MgB2 bulks doped with 3D-CNS with molar ratio composition of MgB2−x(3D-CNS)x (x = 0, 0.1 and 0.2) have been prepared by in situ sintering process. The microstructure, critical current density and flux pinning of the sintered samples have been investigated. Differing from the structure in previous studies, the 3D-CNS doping is more efficient for the refinement of the MgB2 grains due to the 3D network structures. The results clearly show that more active C releasing from 3D-CNS at high temperature can provide effective flux pinning centers by the substitution of C for B in MgB2 lattice. Furthermore, the lattice distortion and increased grain boundaries should be responsible for the enhancement of critical current density (Jc) at high magnetic fields as well as the increased irreversible magnetic field (Hirr). However, the positive action in Jc at low field has been extremely offset by the concentration of impurities at MgB2 grain boundaries such as released extra C without substitution and MgO, which is considered to further deteriorate the grain connectivity.
Solid Particle Erosion Studies of Varying Tow-Scale Carbon Fibre-Reinforced Polymer Composites
Authors: Shanmugam Suresh Kumar , Sundaresan Thirumalai Kumaran , Varol Temel , Kurniawan Rendi
Published: October 2022

Solid particle erosion inevitably occurs if a gas–solid or liquid–solid mixture is in contact with a surface, e.g., in pneumatic conveyors. Nowadays, an erosive failure of the component after the usage of a long period has been gaining the interest of the researchers. In this research work, carbon fibre-reinforced polymer (CFRP) composites are prepared by varying the tow sizes of fibres, such as 5k, 10k, and 15k. The prepared composites are subjected to erosion studies by varying the process parameters, such as the impact angle (30, 60, and 90 degrees) and velocity (72, 100, and 129 m/s). The Taguchi orthogonal array design has been employed for the experimental plan and the erosion rate and surface roughness are observed for each run. The changes in the responses are reported for varying process parameters. The higher erodent velocity of 129m/s leads to higher erosion rates and forms poor surface quality. The minimum impact angle of 30 degrees provides higher erosion rates and higher surface roughness than the other impingement angles. Finally, the eroded surface of each sample is examined through microscopic and 3D profilometer images and the erosion mechanism is analysed at different conditions. The eroded particles supplied at lower speeds do not penetrate the composite surface. However, it is well-known that the lower the collision force, the harder the traces on the surface, yet no sign of fibre breaking or pull-out is observed. The passage of erodent particles on the composite caused surface waviness (flow trace), which prevents the surface from degrading.
Bimetallic Thin-Walled Box Beam Thermal Buckling Response
Authors: Simonetti Sandra Kvaternik , Turkalj Goran , Banić Damjan , Lanc Domagoj
Published: October 2022

A beam model for thermal buckling analysis of a bimetallic box beam is presented. The Euler–Bernoulli–Vlasov beam theory is employed considering large rotations but small strains. The nonlinear stability analysis is performed using an updated Lagrangian formulation. In order to account for the thermal effects of temperature-dependent (TD) and temperature-independent (TID) materials, a uniform temperature rise through beam wall thickness is considered. The numerical results for thin-walled box beams are presented to investigate the effects of different boundary conditions, beam lengths and material thickness ratios on the critical buckling temperature and post-buckling responses. The effectiveness and accuracy of the proposed model are verified by means of comparison with a shell model. It is revealed that all of the abovementioned effects are invaluable for buckling analysis of thin-walled beams under thermal load. Moreover, it is shown that the TD solutions give lower values than the TID one, emphasizing the importance of TD materials in beams.
High Strain Rate Quasi-Superplasticity Behavior in an Ultralight Mg-9.55Li-2.92Al-0.027Y-0.026Mn Alloy Fabricated by Multidirectional Forging and Asymmetrical Rolling
Authors: Cao Furong , Shang Huihui , Guo Nanpan , Kong Shuting , Liu Renjie
Published: October 2022

To explore new approaches to severe plastic deformation and the ductility of a multicomponent magnesium–lithium alloy, an ultralight microduplex Mg-9.55Li-2.92Al-0.027Y-0.026Mn alloy was made by novel multidirectional forging and asymmetrical rolling, and the superplasticity behavior was investigated by optical microscope, hot tensile test, and modeling. The average grain size is 1.9 μm in this alloy after multidirectional forging and asymmetrical rolling. Remarkable grain refinement caused by such a forming, which turns the as-cast grain size of 144.68 μm into the as-rolled grain size of 1.9 μm, is achieved. The elongation to failure of 228.05% is obtained at 523 K and 1 × 10−2 s−1, which demonstrates the high strain rate quasi-superplasticity. The maximum elongation to failure of 287.12% was achieved in this alloy at 573 K and 5 × 10−4 s−1. It was found that strain-induced grain coarsening at 523 K is much weaker than the strain-induced grain coarsening at 573 K. Thus, the ductility of 228.05% is suitable for application in high strain rate superplastic forming. The stress exponent of 3 and the average activation energy for deformation of 50.06 kJ/mol indicate that the rate-controlling deformation mechanism is dislocation-glide controlled by pipe diffusion.
Fatigue in an AZ31 Alloy Subjected to Rotary Swaging
Authors: Trojanová Zuzanka , Drozd Zdeněk , Halmešová Kristýna , Džugan Ján , Hofrichterová Tereza , Palček Peter , Minárik Peter , Škraban Tomáš , Nový František
Published: October 2022

The magnesium AZ31 alloy was swaged with rotary pressure with the aim of redefining the microstructure and improving mechanical and fatigue properties. The rotary swaging process and subsequent ageing improved the yield stress in tension and compression. In the present study, the investigation was focused on fatigue behaviour. The samples were cycled in a symmetric regime with a frequency of 35 Hz. A dependence of the stress amplitude on the number of cycles up to the fracture was estimated. The microstructure of the samples and fracture surfaces was analysed with a scanning electron microscope. The fatigue process was influenced by the pronounced texture formed in the swaging process. The fatigue properties of the swaged samples improved substantially—the endurance limit based on 107 cycles was approximately 120 MPa—compared to those of the cast alloy. The analysis of the fracture surfaces showed a transcrystalline fatigue fracture.
Prediction of Structural Behavior of Continuous Reinforced Concrete Beams with Hybrid CFRP-Steel Bars
Authors: Pang Miao , Dong Yi , Liu Xing , Sun Wei , Lou Tiejiong
Published: October 2022

The present investigation aims to identify the flexural performance of two-span concrete beams reinforced with hybrid carbon fiber reinforced polymer (CFRP) and steel bars. By applying a finite element analysis, a comprehensive numerical assessment is performed. The investigated variables are Af/Ar (Af = area of CFRP bars; Ar = total area of CFRP/steel bars), load pattern (symmetrical and unsymmetrical loading) and load type (center-point, third-point and uniform loading). The results show that beams with Af/Ar of 0.25 show 16.0% and 11.3% higher ultimate load at symmetrical and unsymmetrical loading, respectively, than beams with Af/Ar of 0.0 (i.e., beams with steel bars), but the change in ultimate load is not apparent when varying Af/Ar between 0.25 and 1.0. Unsymmetrical loading causes 6.0–15.0% greater deflection capacities than the symmetrical one. When Af/Ar increases from 0.0 to 1.0, moment redistribution at symmetrical loading is decreased significantly by 62%, while the redistribution variation is marginal at unsymmetrical loading. In addition, the applicability of two equations based on the ultimate strain in tensile bars for predicting moment redistribution is evaluated. It is generally shown that these equations can account for the influence of Af/Ar and load type.
Defect Detection of Pipeline Inner Surface Based on Coaxial Digital Image Correlation with Hypercentric Lens
Authors: Qi Jiankang , Xu Mengqiao , Zhang Weiling , Liu Yubo , Dai Xiangjun
Published: October 2022

A coaxial dual-camera digital image correlation system using a hypercentric lens was proposed to determine the defect position in the inner wall of a pipeline under loads. Compared with the traditional dual-camera system, this system ensures that both cameras can capture a 360-degree panoramic image in the same position. Herein, the imaging principle of the system was introduced in detail. In addition, the effectiveness and accuracy of the proposed method were verified through verification and application experiments.
The Impact of Plastic Deformation on the Microstructure and Tensile Strength of Haynes 282 Nickel Superalloy Produced by DMLS and Casting
Authors: Sitek Ryszard , Puchlerska Sandra , Nejman Ilona , Majchrowicz Kamil , Pakieła Zbigniew , Żaba Krzysztof , Mizera Jarosław
Published: October 2022

The article presents the results of research on the influence of plastic deformation on the microstructure and tensile strength of Haynes 282 nickel superalloy produced by direct metal laser sintering (DMLS) and a conventional technique (casting). Samples were tested for dimensional accuracy using a 3D scanner. Then, the samples were subjected to plastic deformation by rolling. The microstructures of the DMLS and the as-cast samples were analysed using a scanning electron microscope. The strength properties of the samples were determined in a static tensile test. Microhardness measurements of the samples were also performed. Based on the analysis of the dimensional accuracy, it was found that the surface quality of the components produced by DMLS is dependent on the input parameters of the 3D printing process. Using the DMLS method, it is possible to produce Haynes 282 with a fine-crystalline microstructure containing dendrites. The fine-crystalline dendritic microstructure and low porosity showed very good tensile strength compared to the as-cast material. It was also found that the increase in the degree of plastic deformation of the as-cast Haynes 282 and the samples produced by the DMLS technique resulted in an increase in the strength of the tested samples, with reduced ductility.
Sol-Gel Synthesis and Characterization of Yttrium-Doped MgFe2O4 Spinel
Authors: Karoblis Dovydas , Mazeika Kestutis , Raudonis Rimantas , Zarkov Aleksej , Kareiva Aivaras
Published: October 2022

In this study, an environmentally friendly sol-gel synthetic approach was used for the preparation of yttrium-doped MgFe2O4. Two series of compounds with different iron content were synthesized and A-site substitution effects were investigated. In the first series, the iron content was fixed and the charge balance was suggested to be compensated by a partial reduction of Fe3+ to Fe2+ or formation of interstitial O2− ions. For the second series of samples, the iron content was reduced in accordance with the substitution level to compensate for the excess of positive charge, which accumulates due to replacing divalent Mg2+ with trivalent Y3+ ions. Structural, morphological and magnetic properties were inspected. It was observed that single-phase compounds can only form when the substitution level reaches 20 mol% of Y3+ ions and iron content is reduced. The coercivity as well as saturation magnetization decreased with the increase in yttrium content. Mössbauer spectroscopy was used to investigate the iron content in both tetrahedral and octahedral positions.
Effect of the Sliding Element Surface Topography on the Oil Film Thickness in EHD Lubrication in Non-Conformal Contact
Authors: Galda Lidia , Sep Jaroslaw , Swirad Slawomir
Published: October 2022

Under hard operating conditions such as high load, low speed and a lack of a sufficient quantity of lubricant, the sliding pairs could suffer serious damage. One of the methods that improves the tribological performance of sliding elements in hard operating conditions is the appropriate surface creation that keeps lubricating substance in cavities. This article presents the results obtained in experimental investigations of the oil film thickness in lubricated non-conformal contact with a different surface topography of the sliding element. The tests were conducted on a ball-on-disc instrument equipped with colorimetric interferometry. Balls of diameter equaled to 19.05 mm were produced from 100 Cr6 steel. To provide hard operating conditions, the glass disc rotated at small speeds in the range of 0.1–0.2 m/s. The tests were carried out at loads of 20 N and 30 N. As a result, in most cases, the highest minimum and average oil film thickness values were obtained when the surface of steel balls was characterized by high negative asymmetry with mainly shallow cavities and some valleys of great depth compared to the height of the peaks. The modified sliding surface that had better performance comprised a comparatively small number of peaks and the curvature of the peaks were large.