bluuebunny/crossref_metadata_2025 huggingface dataset:
Measuring Software Modularity Based on Software Networks
Authors: Xiang Yiming , Pan Weifeng , Jiang Haibo , Zhu Yunfang , Li Hao
Published: March 2019

Modularity has been regarded as one of the most important properties of a successful software design. It has significant impact on many external quality attributes such as reusability, maintainability, and understandability. Thus, proposing metrics to measure the software modularity can be very useful. Although several metrics have been proposed to characterize some modularity-related attributes, they fail to characterize software modularity as a whole. A complex network uses network models to abstract the internal structure of complex systems, providing a general way to analyze complex systems as a whole. In this paper, we introduce the complex network theory into software engineering and employ modularity, a metric widely used in the field of community detection in complex network research, to measure software modularity as a whole. First, a specific piece of software is represented by a software network, feature coupling network (FCN), where methods and attributes are nodes, couplings between methods and attributes are edges, and the weight on the edges denotes the coupling strength. Then, modularity is applied to the FCN to measure software modularity. We apply the Weyuker’s criteria which is widely used in the field of software metrics, to validate the modularity as a software metric theoretically, and also perform an empirical evaluation using open-source Java software systems to show its effectiveness as a software metric to measure software modularity.
CDCS: Cluster-Based Distributed Compressed Sensing to Facilitate QoS Routing in Cognitive Video Sensor Networks
Authors: Shen Hang , Li Lingli , Wang Tianjing , Bai Guangwei
Published: March 2019

Compressed sensing based in-network compression methods which minimize data redundancy are critical to cognitive video sensor networks. However, most existing methods require a large number of sensors for each measurement, resulting in significant performance degradation in energy efficiency and quality-of-service satisfaction. In this paper, a cluster-based distributed compressed sensing scheme working together with a quality-of-service aware routing framework is proposed to deliver visual information in cognitive video sensor networks efficiently. First, the correlation among adjacent video sensors determines the member nodes that participate in a cluster. On this basis, a sequential compressed sensing approach is applied to determine whether enough measurements are obtained to limit the reconstruction error between decoded signals and original signals under a specified reconstruction threshold. The goal is to maximize the removal of unnecessary traffic without sacrificing video quality. Lastly, the compressed data is transmitted via a distributed spectrum-aware quality-of-service routing scheme, with an objective of minimizing energy consumption subject to delay and reliability constraints. Simulation results demonstrate that the proposed approach can achieve energy-efficient data delivery and reconstruction accuracy of visual information compared with existing quality-of-service routing schemes.
On the Use of PDF-3D to Overcome Spatial Visualization Difficulties Linked with Ternary Phase Diagrams
Authors: Vergara Diego , Rubio Manuel Pablo , Lorenzo Miguel
Published: March 2019

Despite the interesting applications that the PDF-3D offers in teaching, especially for subjects related to spatial comprehension difficulties, such a didactic tool is not well known in the education sector. Thus, a proposal of using PDF-3D in engineering studies is presented in this paper, specifically, in the field of teaching ternary phase diagrams (TPDs). The didactic resource—easy to design and easy to use—allows students to overcome spatial visualization difficulties linked with TPDs. According to students’ opinions, the PDF-3D is an effective tool to use in any topic related to spatial difficulties and, in addition, is a friendly and easy-to-use tool. This fact and the simplicity of designing a PDF-3D make it a useful tool for educational aims.
An Efficient Pilot Assignment Scheme for Addressing Pilot Contamination in Multicell Massive MIMO Systems
Authors: Al-hubaishi Ahmed S. , Noordin Nor Kamariah , Sali Aduwati , Subramaniam Shamala , Mohammed Mansoor Ali
Published: March 2019

The reuse of the same pilot group across cells to address bandwidth limitations in a network has resulted in pilot contamination. This causes severe inter-cell interference at the targeted cell. Pilot contamination is associated with multicell massive multiple-input multiple-output (MIMO) systems which degrades the system performance even when extra arrays of antennas are added to the network. In this paper, we propose an efficient pilot assignment (EPA) scheme to address this issue by maximizing the minimum uplink rate of the target cell’s users. To achieve this, we exploit the large-scale characteristics of the fading channel to minimize the amount of outgoing inter-cell interference at the target cell. Results from the simulation show that the EPA scheme outperforms both the conventional and the smart pilot assignment (SPA) schemes by reducing the effect of inter-cell interference. These results, show that the EPA scheme has significantly improved the system performance in terms of achievable uplink rate and cumulative distribution function (CDF) for both signal-to-interference-plus-noise ratio (SINR), and uplink rate.
Comparison of MOCVD and MBE Regrowth for CAVET Fabrication
Authors: Kotzea Simon , Witte Wiebke , Godejohann Birte-Julia , Marx Mathias , Heuken Michael , Kalisch Holger , Aidam Rolf , Vescan Andrei
Published: March 2019

In this paper, we demonstrate the fabrication of current aperture vertical electron transistors (CAVET) realized with two different epitaxial growth methods. Templates with a p-GaN current blocking layer (CBL) were deposited by metal organic chemical vapor deposition (MOCVD). Channel and barrier layers were then regrown by either molecular beam epitaxy (MBE) or MOCVD. Scanning electron microscope (SEM) images and atomic force microscope (AFM) height profiles are used to identify the different regrowth mechanisms. We show that an AlN interlayer below the channel layer was able to reduce Mg diffusion during the high temperature MOCVD regrowth process. For the low-temperature MBE regrowth, Mg diffusion was successfully suppressed. CAVET were realized on the various samples. The devices suffer from high leakage currents, thus further regrowth optimization is needed.
Software Architectures for Smart Grid System—A Bibliographical Survey
Authors: Ananthavijayan Ramesh , Karthikeyan Shanmugam Prabhakar , Padmanaban Sanjeevikumar , Holm-Nielsen Jens Bo , Blaabjerg Frede , Fedak Viliam
Published: March 2019

Smart grid software interconnects multiple Engineering disciplines (power systems, communication, software and hardware technology, instrumentation, big data, etc.). The software architecture is an evolving concept in smart grid systems, in which system architecture development is a challenging process. The architecture has to realize the complex legacy power grid systems and cope with current Information and Communication Technologies (ICT). The distributed generation in a smart grid environment expects the software architecture to be distributed and to enable local control. Smart grid architecture should also be modular, flexible, and adaptable to technology upgrades. In this paper, the authors have made a comprehensive review of architectures for smart grids. An in depth analysis of layered and agent-based architectures based on the National Institute of Standards and Technology (NIST) conceptual model is presented. Also presented is a set of smart grid Reference Architectures dealing with cross domain technology.
Numerical Investigation of Fracture Compressibility and Uncertainty on Water-Loss and Production Performance in Tight Oil Reservoirs
Authors: Liao Kai , Zhang Shicheng , Ma Xinfang , Zou Yushi
Published: March 2019

Multi-stage hydraulic fracturing along with horizontal wells are widely used to create complex fracture networks in tight oil reservoirs. Analysis of field flowback data shows that most of the fracturing fluids are contained in a complex fracture network, and fracture-closure is the main driving mechanism during early clean up. At present, the related fracture parameters cannot be accurately obtained, so it is necessary to study the impacts of fracture compressibility and uncertainty on water-loss and the subsequent production performance. A series of mechanistic models are established by considering stress-dependent porosity and permeability. The impacts of fracture uncertainties, such as natural fracture density, proppant distribution, and natural fracture heterogeneity on flowback and productivity are quantitatively assessed. Results indicate that considering fracture closure during flowback can promote water imbibition into the matrix and delay the oil breakthrough time compared with ignoring fracture closure. With the increase of natural fracture density, oil breakthrough time is advanced, and more water is retained underground. When natural fractures connected with hydraulic fractures are propped, well productivity will be enhanced, but proppant embedment can cause a loss of oil production. Additionally, the fracture network with more heterogeneity will lead to the lower flowback rate, which presents an insight in the role of fractures in water-loss.
A Power Converter Decoupled from the Resonant Network for Wireless Inductive Coupling Power Transfer
Authors: Chen Lin , Hong Jianfeng , Guan Mingjie , Wu Wei , Chen Wenxiang
Published: March 2019

In a traditional inductive coupling power transfer (ICPT) system, the converter and the resonant network are strongly coupled. Since the coupling coefficient and the parameters of the resonant network usually vary, the resonant network easily detunes, and the system efficiency, power source capacity, power control, and soft switching conditions of the ICPT system are considerably affected. This paper presents an ICPT system based on a power converter decoupled from the resonant network. In the proposed system, the primary inductor is disconnected from the resonant network during the energy injection stage. After storing a certain amount of energy, the primary inductor is reconnects with the resonant network. Through this method, the converter can be decoupled from the resonant network, and the resonant network can be tuned under various coupling coefficients. Theoretical analysis was explored first. Simulations and experimental work are carried out to verify the theoretical analysis. The results show that the proposed ICPT system has the virtues of low power source capacity, independent power control, and soft switching operation under different coupling coefficients.
Novel Design of Primary Optical Elements Based on a Linear Fresnel Lens for Concentrator Photovoltaic Technology
Authors: Pham Thanh Tuan , Vu Ngoc Hai , Shin Seoyong
Published: March 2019

In this paper, we present a design and optical simulation of a novel linear Fresnel lens. The lens can be applied to a concentrator photovoltaic (CPV) system as a primary optical element (POE) to increase the concentration ratio and improve the uniformity of irradiance distribution over the receiver. In addition, the CPV system can use the proposed lens as a concentrator without involving a secondary optical element (SOE). The designed lens, which is a combination of two linear Fresnel lenses placed perpendicular to each other, can collect and distribute the direct sunlight on two dimensions. The lens is first designed in the MATLAB program, based on the edge ray theorem, Snell’s law, and the conservation of the optical path length, and then drawn in three dimensions (3D) by using LightToolsTM. Furthermore, in order to optimize the structure and investigate the performance of the lens, the ray tracing and the simulation are also performed in LightToolsTM. The results show that the newly designed lens can achieve a high concentration ratio of 576 times, a high optical efficiency of 82.4%, an acceptable tolerance of 0.84°, and high uniform irradiance of around 77% for both horizontal and vertical investigation lines over the receiver.
A Nonlinear Analytical Algorithm for Predicting the Probabilistic Mass Flow of a Radial District Heating Network
Authors: Sun Guoqiang , Wang Wenxue , Wu Yi , Hu Wei , Yang Zijun , Wei Zhinong , Zang Haixiang , Chen Sheng
Published: March 2019

This paper develops a nonlinear analytical algorithm for predicting the probabilistic mass flow of radial district heating networks based on the principle of heat transfer and basic pipe network theory. The use of a nonlinear mass flow model provides more accurate probabilistic operation information for district heating networks with stochastic heat demands than existing probabilistic power flow analytical algorithms based on a linear mass flow model. Moreover, the computation is efficient because our approach does not require repeated nonlinear mass flow calculations. Test results on a 23-node district heating network case indicate that the proposed approach provides an accurate and efficient estimation of probabilistic operation conditions.
Research on Network Intrusion Detection Based on Incremental Extreme Learning Machine and Adaptive Principal Component Analysis
Authors: Gao Jianlei , Chai Senchun , Zhang Baihai , Xia Yuanqing
Published: March 2019

Recently, network attacks launched by malicious attackers have seriously affected modern life and enterprise production, and these network attack samples have the characteristic of type imbalance, which undoubtedly increases the difficulty of intrusion detection. In response to this problem, it would naturally be very meaningful to design an intrusion detection system (IDS) to effectively and quickly identify and detect malicious behaviors. In our work, we have proposed a method for an IDS-combined incremental extreme learning machine (I-ELM) with an adaptive principal component (A-PCA). In this method, the relevant features of network traffic are adaptively selected, where the best detection accuracy can then be obtained by I-ELM. We have used the NSL-KDD standard dataset and UNSW-NB15 standard dataset to evaluate the performance of our proposed method. Through analysis of the experimental results, we can see that our proposed method has better computation capacity, stronger generalization ability, and higher accuracy.
Antioxidant Activity, Total Polyphenol, Flavonoid and Tannin Contents of Fermented Green Coffee Beans with Selected Yeasts
Authors: Haile Mesfin , Kang Won Hee
Published: March 2019

We examined the antioxidant activity, total polyphenol content (TPC), total flavonoid content (TFC), total tannin content (TTC) and physical characteristics of green coffee beans fermented with selected yeasts. There was no significant (p > 0.05) interaction effect between yeast-fermented coffee extracts and duration of fermentation on antioxidant activity (oxygen radical absorbance capacity [ORAC] and superoxide dismutase-like [SOD-like] activity). However, the mean of the antioxidant activity (ORAC and SOD-like activity) significantly (p < 0.05) increased in the fermented coffee extracts compared to unfermented coffee. There were significant (p < 0.05) interaction effects between yeast-fermented coffee extracts and duration of fermentation (24 h and 48 h) on the TPC, TFC, TTC and pH of the fermented solution and on the colors of the ground-roasted coffee. The TPC showed a pattern of increase in samples Ferm-1 and Ferm-3 as fermentation time increased from 24 h to 48 h. However, a decreasing TPC trend was observed in Ferm-2 as the number of fermentation hours increased from 24 to 48. The fermented coffee beans had a significantly higher flavonoid content than the unfermented coffee beans, while fermentation significantly decreased the tannin content compared to that in unfermented coffee.
Quasi-Static and Low-Velocity Impact Behavior of Intraply Hybrid Flax/Basalt Composites
Authors: Sarasini Fabrizio , Tirillò Jacopo , Ferrante Luca , Sergi Claudia , Russo Pietro , Simeoli Giorgio , Cimino Francesca , Ricciardi Maria Rosaria , Antonucci Vincenza
Published: March 2019

In an attempt to increase the low-velocity impact response of natural fiber composites, a new hybrid intraply woven fabric based on flax and basalt fibers has been used to manufacture laminates with both thermoplastic and thermoset matrices. The matrix type (epoxy or polypropylene (PP) with or without a maleated coupling agent) significantly affected the absorbed energy and the damage mechanisms. The absorbed energy at perforation for PP-based composites was 90% and 50% higher than that of epoxy and compatibilized PP composites, respectively. The hybrid fiber architecture counteracted the influence of low transverse strength of flax fibers on impact response, irrespective of the matrix type. In thermoplastic laminates, the matrix plasticization delayed the onset of major damage during impact and allowed a better balance of quasi-static properties, energy absorption, peak force, and perforation energy compared to epoxy-based composites.
Preliminary Monitoring of Praziquantel in Water and Sediments at a Japanese Amberjack (Seriola quinqueradiata) Aquaculture Site
Authors: Ido Atsushi , Kanemaru Motohisa , Tanioka Yoshiharu
Published: March 2019

Praziquantel (PZQ), an active compound against Platyhelminthes, is an essential anthelmintic for the aquaculture industry. However, there are few reports of the environmental risks of PZQ use in aquaculture. In this study, we monitored PZQ in water and sediment at an aquaculture site of Japanese amberjack, also called yellowtail (Seriola quinqueradiata). Although PZQ was detected in water during treatment and 3 days post-treatment, PZQ levels were below the detectable limit in water 60 m from the net pen during the treatment, and in all sediment samples. In this preliminary study, we could not detect residue of PZQ from sediments in the aquaculture site, and no evidence about environmental effect of PZQ administration was obtained.
Effect of the Plastein Reaction in Presence of Extrinsic Amino Acids on the Protective Activity of Casein Hydrolysate against Ethanol-Induced Damage in HHL-5 Cells
Authors: Bo Li-Ying , Pang Jia-Nan , Song Chun-Li , Li Tie-Jing
Published: March 2019

Casein hydrolysates (CH) were prepared using papain and modified by the plastein reaction (CH-P) in the presence of extrinsic phenylalanine (CH-P-Phe) or tryptophan (CH-P-Trp). The in vitro protective activity of CH and its modified products against ethanol-induced damage in HHL-5 cells was investigated. The results showed that the modification by the plastein reaction reduced the amino group content of CH. However, the modification by the plastein reaction in the presence of extrinsic amino acids could enhance the antioxidant, proliferative, cell cycle arresting, and anti-apoptosis activity of CH. Biological activities of CH and its modified products in the HHL-5 cells varied depending on the hydrolysate concentration (1, 2, and 3 mg/mL) and treatment time (24, 48, and 72 h). Generally, higher biological activities were found after cell treatment with CH or its modified products at concentration of 2 mg/mL for 48 h compared to other treatments. In addition, CH modified in the presence of tryptophan (CH-P-Trp) showed higher biological activity than that modified in the presence of phenylalanine (CH-P-Phe). Based on the obtained results, it can be concluded that casein hydrolysates with enhanced biological activity and potential health benefits can be produced by papain and the plastein reaction with the incorporation of extrinsic amino acids.
VvSWEET10 Mediates Sugar Accumulation in Grapes
Authors: Zhang Zhan , Zou Luming , Ren Chong , Ren Fengrui , Wang Yi , Fan Peige , Li Shaohua , Liang Zhenchang
Published: March 2019

Sugar accumulation is a critical event during grape berry ripening that determines the grape market values. Berry cells are highly dependent on sugar transporters to mediate cross-membrane transport. However, the role of sugar transporters in improving sugar accumulation in berries is not well established in grapes. Herein we report that a Sugars Will Eventually be Exported Transporter (SWEET), that is, VvSWEET10, was strongly expressed at the onset of ripening (véraison) and can improve grape sugar content. VvSWEET10 encodes a plasma membrane-localized transporter, and the heterologous expression of VvSWEET10 indicates that VvSWEET10 is a hexose-affinity transporter and has a broad spectrum of sugar transport functions. VvSWEET10 overexpression in grapevine calli and tomatoes increased the glucose, fructose, and total sugar levels significantly. The RNA sequencing results of grapevine transgenic calli showed that many sugar transporter genes and invertase genes were upregulated and suggest that VvSWEET10 may mediate sugar accumulation. These findings elucidated the role of VvSWEET10 in sugar accumulation and will be beneficial for the improvement of grape berry quality in the future.
Nomadic Narrative in Charlotte Brontë’s Villette
Authors: Kim Jungah
Published: March 2019

Various critics have examined Charlotte Brontë’s Villette’s missing ending as a proof of Lucy Snowe’s unreliability in leaving the narrative purposefully ambiguous to escape her possible negative ending. I, however, interpret the ending as one of the ways in which she actively and positively refuses the concept of closure, and rather, creates, what I would call, a nomadic narrative. Nomadic narrative is term I coined based on the idea of Rosi Braidotti’s nomadic theory and Georg Lukács’s The Theory of the Novel to re-imagine Lucy’s narration and narrative, not as a concealment, but as an embracement of her nomadic subjectivity and acknowledgement that she has no true end. I further argue that nomadic narrative is a narrative that fractures and recreates itself through its gaps and rewritten portions, gaining its own sense of agency. Unlike narratives that only fixate on protagonists, nomadic narrative becomes an open and posthuman space that allows the incorporation of nonhuman subjects.
Influence of Work on Elevated Blood Pressure in Hispanic Adolescents in South Texas
Authors: Shipp Eva M. , Cooper Sharon P. , Jiang Luohua , Trueblood Amber B. , Ross Jennifer
Published: March 2019

Literature supports an association between work and cardiovascular disease in adults. The objective of this study was to examine the relationship between current work status and elevated blood pressure in Hispanic adolescents. Participants were students in Hidalgo County, located along the Texas-Mexico border. Participants enrolled in the cohort study in ninth grade with assessments completed once a year for up to three years. Participants completed a self-report survey, while staff measured height, weight, waist circumference, blood pressure, and were screened for acanthosis nigricans. A generalized linear regression model with a logit link function was constructed to assess current work status and elevated blood pressure. Of the 508 participants, 29% had elevated blood pressure, which was associated with being male and other chronic disease indicators (e.g., acanthosis nigricans, overweight/obesity). The mean probability for elevated blood pressure was higher among currently working adolescents compared to those who were not. Findings were statistically significant (p < 0.05) at baseline. The findings illustrate that a large proportion of adolescents along the Texas-Mexico border may have elevated blood pressure and that working may be associated with it. Subsequent research is needed to confirm these findings, as well as to identify the mechanism for how work may increase hypertension in adolescents.
Spatiotemporal Pattern of Fine Particulate Matter and Impact of Urban Socioeconomic Factors in China
Authors: Shi Tuo , Liu Miao , Hu Yuanman , Li Chunlin , Zhang Chuyi , Ren Baihui
Published: March 2019

Frequent hazy weather has been one of the most obvious air problems accompanying China’s rapid urbanization. As one of the main components of haze pollution, fine particulate matter (PM2.5), which severely affects environmental quality and people’s health, has attracted wide attention. This study investigated the PM2.5 distribution, changing trends and impact of urban factors based on remote-sensing PM2.5 concentration data from 2000 to 2015, combining land-use data and socioeconomic data, and using the least-squares method and structural equation model (SEM). The results showed that the high concentration of PM2.5 in China was mainly concentrated in the eastern part of China and Sichuan Province. The trends of the PM2.5 concentration in eastern part and Northeast China, Sichuan, and Guangxi Provinces were positive. Meanwhile, the ratios of increasing trends were strongest in built-up land and agricultural land, and the decreasing trends were strongest in forest and grassland, but the overall trends were still growing. The SEM results indicated that economic factors contributed most to PM2.5 pollution, followed by demographic factors and spatial factors. Among all observed variables, the secondary industrial GDP had the highest impact on PM2.5 pollution. Based on the above results, PM2.5 pollution remains an important environmental issue in China at present and even in the future. It is necessary for decision-makers to make actions and policies from macroscopic and microscopic, long-term and short-term aspects to reduce pollution.
Characteristics of Canteens at Elementary Schools, Upper Secondary Schools and Workplaces that Comply with Food Service Guidelines and Have a Greater Focus on Food Waste
Authors: Lassen Anne , Christensen Lene , Spooner Max , Trolle Ellen
Published: March 2019

Policy actions to improve the nutritional environment include the provision of official food service guidelines. This study aimed to examine compliance with food service guidelines for hot meals as well as self-evaluated focus on food waste reduction across settings, i.e., elementary schools, upper secondary schools and workplaces, and different canteen characteristics. The same five criteria for hot meals were applied for all settings with regard to serving of fruit and vegetables, fish, wholegrain product and high fat meat and dairy products. A self-administered questionnaire survey was conducted as a cross-sectional study among 680 Danish canteens. Canteens having a high degree of organic food procurement were more likely to comply with the five criteria for hot meals combined (OR 2.00 (Cl 1.13,3.53)). Also, the use of organic food together with having a meal policy was associated with reported focus on food waste reduction (OR 1.91 (CI 1.12,3.25) and 1.84 (Cl 1.31,2.59), respectively). Compliance with individual criteria varied across settings with elementary schools being more likely to comply with criteria on, e.g., maximum serving of non-wholegrain products, whereas workplaces were more likely to comply with criteria on, e.g., minimum fruit and vegetable content and serving of fish. In addition, specific characteristics, e.g., serving system, were found to predict compliance with some of the criteria. These findings highlight the need to address differences in canteen characteristics when planning implementation support for both guideline and food waste reduction initiatives.
MicroRNA Let-7d-3p Contributes to Cardiac Protection via Targeting HMGA2
Authors: Wong Lee Lee , Saw Eng Leng , Lim Jia Yuen , Zhou Yue , Richards Arthur Mark , Wang Peipei
Published: March 2019

We tested the hypothesis that Let-7d-3p contributes to cardiac cell protection during hypoxic challenge. Myoblast H9c2 cells and primary neonatal rat ventricular cardiomyocytes (NRVM) were transfected with five selected miRNA mimics. Both cell lines were subjected to 0.2% oxygen hypoxia. The protective effects of these miRNAs were determined by assessment of cell metabolic activity by CCK8 assay and measurement of lactate dehydrogenase (LDH) release as a marker of cell injury. Apoptosis and autophagy flux were assessed by Annexin V/7-AAD double staining and the ratio of LC3 II/I with Baf-A1 treatment, an autophagy flux inhibitor, respectively. Luciferase-reporter assay, RT-qPCR and Western blots were performed to identify the changes of relevant gene targets. Among five miRNA mimic transfections, Let-7d-3p increased CCK8 activity, and decreased LDH release in both H9c2 and NRVM during hypoxia. Apoptosis was significantly reduced in H9c2 cells transfected with Let-7d-3p mimic. Autophagy and autophagy flux were not affected. In silico, mRNAs of HMGA2, YY1, KLF9, KLF12, and MEX3C are predicted targets for Let-7d-3p. Luciferase-reporter assay confirmed that Let-7d-3p bound directly to the 3’-UTR region of HMGA2, MEX3C, and YY1, the down-regulations of these mRNAs were verified in both H9c2 and NRVM. The protein expression of HMGA2, but not others, was downregulated in H9c2 and NRVM. It is known that HMGA2 is a strong apoptosis trigger through the blocking of DNA repair. Thus, we speculate that the anti-apoptotic effects of Let-7d-3p mimic during hypoxia challenge are due to direct targeting of HMGA2.
The BET Bromodomain Inhibitor I-BET-151 Induces Structural and Functional Alterations of the Heart Mitochondria in Healthy Male Mice and Rats
Authors: Piquereau Jérôme , Boet Angèle , Péchoux Christine , Antigny Fabrice , Lambert Mélanie , Gressette Mélanie , Ranchoux Benoît , Gambaryan Natalia , Domergue Valérie , Mumby Sharon , Montani David , Adcock Ian M. , Humbert Marc , Garnier Anne , Rucker-Martin Catherine , Perros Frédéric
Published: March 2019

The bromodomain and extra-terminal domain family inhibitors (BETi) are a promising new class of anticancer agents. Since numerous anticancer drugs have been correlated to cardiomyopathy, and since BETi can affect non-cancerous tissues, we aimed to investigate in healthy animals any ultrastructural BETi-induced alterations of the heart as compared to skeletal muscle. Male Wistar rats were either treated during 3 weeks with I-BET-151 (2 or 10 mg/kg/day) (W3) or treated for 3 weeks then allowed to recover for another 3 weeks (W6) (3-weeks drug washout). Male C57Bl/6J mice were only treated during 5 days (50 mg/kg/day). We demonstrated the occurrence of ultrastructural alterations and progressive destruction of cardiomyocyte mitochondria after I-BET-151 exposure. Those mitochondrial alterations were cardiac muscle-specific, since the skeletal muscles of exposed animals were similar in ultrastructure presentation to the non-exposed animals. I-BET-151 decreased the respiration rate of heart mitochondria in a dose-dependent manner. At the higher dose, it also decreased mitochondrial mass, as evidenced by reduced right ventricular citrate synthase content. I-BET-151 reduced the right and left ventricular fractional shortening. The concomitant decrease in the velocity-time-integral in both the aorta and the pulmonary artery is also suggestive of an impaired heart function. The possible context-dependent cardiac side effects of these drugs have to be appreciated. Future studies should focus on the basic mechanisms of potential cardiovascular toxicities induced by BETi and strategies to minimize these unexpected complications.
The First Transcriptome Assembly of Yenyuan Stream Salamander (Batrachuperus yenyuanensis) Provides Novel Insights into Its Molecular Evolution
Authors: Xiong Jianli , Lv Yunyun , Huang Yong , Liu Qiangqiang
Published: March 2019

The Yenyuan stream salamander (Batrachuperus yenyuanensis) has been previously evaluated with regards to phylogeny, population genetics, and hematology, but genomic information is sparse due to the giant genome size of salamanders which contain highly repetitive sequences, thus resulting in the lack of a complete reference genome. This study evaluates the encoding genetic sequences and provides the first transcriptome assembly of Yenyuan stream salamander based on mixed samples from the liver, spermary, muscle and spleen tissues. Using this transcriptome assembly and available encoding sequences from other vertebrates, the gene families, phylogenetic status, and species divergence time were compared or estimated. A total of 13,750 encoding sequences were successfully obtained from the transcriptome assembly of Yenyuan stream salamander, estimated to contain 40.1% of the unigenes represented in tetrapod databases. A total of 88.79% of these genes could be annotated to a biological function by current databases. Through gene family clustering, we found multiple possible isoforms of the Scribble gene—whose function is related to regeneration—based on sequence similarity. Meanwhile, we constructed a robust phylogenetic tree based on 56 single-copy orthologues, which indicates that based on phylogenetic position, the Yenyuan stream salamander presents the closest relationship with the Chinese giant salamander (Andrias davidianus) of the investigated vertebrates. Based on the fossil-calibrated phylogeny, we estimated that the lineage divergence between the ancestral Yenyuan stream salamander and the Chinese giant salamander may have occurred during the Cretaceous period (~78.4 million years ago). In conclusion, this study not only provides a candidate gene that is valuable for exploring the remarkable capacity of regeneration in the future, but also gives an interesting insight into the understanding of Yenyuan stream salamander by this first transcriptome assembly.
Exosome-Mediated Delivery of Inducible miR-423-5p Enhances Resistance of MRC-5 Cells to Rabies Virus Infection
Authors: Wang Jingyu , Teng Yawei , Zhao Guanshu , Li Fang , Hou Ali , Sun Bo , Kong Wei , Gao Feng , Cai Linjun , Jiang Chunlai
Published: March 2019

The human diploid cell line Medical Research Council -5 (MRC-5) is commonly utilized for vaccine development. Although a rabies vaccine developed in cultured MRC-5 cells exists, the poor susceptibility of MRC-5 cells to the rabies virus (RABV) infection limits the potential yield of this vaccine. The underlying mechanism of MRC-5 cell resistance to RABV infection remains unknown. In this study, we demonstrate that viral infection increased exosomal release from MRC-5 cells; conversely, blocking exosome release promoted RABV infection in MRC-5 cells. Additionally, RABV infection up-regulated microRNA (miR)-423-5p expression in exosomes, resulting in feedback inhibition of RABV replication by abrogating the inhibitory effect of suppressor of cytokine signaling 3 (SOCS3) on type I interferon (IFN) signaling. Furthermore, intercellular delivery of miR-423-5p by exosomes inhibited RABV replication in MRC-5 cells. We also show that RABV infection increased IFN-β production in MRC-5 cells and that blocking the type I IFN receptor promoted RABV infection. In conclusion, MRC-5 cells were protected from RABV infection by the intercellular delivery of exosomal miR-423-5p and the up-regulation of IFN-β. These findings reveal novel antiviral mechanisms in MRC-5 cells against RABV infection. miR-423-5p, exosomes, and IFN signaling pathways may therefore be potential targets for improving MRC-5 cell-based rabies vaccine production.
Co-Amended Synergistic Interactions between Arbuscular Mycorrhizal Fungi and the Organic Substrate-Induced Cucumber Yield and Fruit Quality Associated with the Regulation of the AM-Fungal Community Structure under Anthropogenic Cultivated Soil
Authors: Ali Ahmad , Ghani Muhammad Imran , Ding Haiyan , Fan Yang , Cheng Zhihui , Iqbal Muhammad
Published: March 2019

Monotonous cucumber double-cropping systems under plastic greenhouse vegetable cultivation (PGVC) previously intensified by long-term anthropogenic activities and manipulative treatments leads to a crop productivity reduction and soil biota disturbances. In this study, the role of the indigenous arbuscular mycorrhizal strain (AM: Glomus versiforme L.) and organic substrate (GS: Garlic stalk) application were assessed for plant microbe interaction and crop productivity feedback in a greenhouse (2016–2018) under a cultivated Anthrosol characterized as a replanted degraded soil. We found that repetitively adding AM inocula with organic substrates (GS) improved the cucumber growth and physiology. The useful trait of AM symbiosis with C-amended organic substrates preferentially manifested as increased root colonization, hyphal density proliferation, AM sporulation, root activity, and suppressed Fusarium incidence. The post AM development further prevailed the synergistic interaction, and the co-inoculation effect resulted in an increase in fruit nutrition uptake, seasonal cucumber yield and fruit quality attributes. Illumina MiSeq analysis of the 18S rRNA gene amplicons revealed that the dominant AM genera that are particularly enriched with the Glomus taxon may be important ecological drivers associated with plant productivity and fruit quality characteristics. These results suggest that the AM-organic substrate association might be a pragmatic option for use as an economic and efficient biological resource and as a newly-sustainable plant microbe mediator to enhance the regional ecosystem services and plant productivity of the anthropogenic PGVC of this region.
Adiponectin in Myopathies
Authors: Gamberi Tania , Magherini Francesca , Fiaschi Tania
Published: March 2019

In skeletal muscle, adiponectin has varied and pleiotropic functions, ranging from metabolic, anti-inflammatory, insulin-sensitizing to regenerative roles. Despite the important functions exerted by adiponectin, the study of the hormone in myopathies is still marginal. Myopathies include inherited and non-inherited/acquired neuromuscular pathologies characterized by muscular degeneration and weakness. This review reports current knowledge about adiponectin in myopathies, regarding in particular the role of adiponectin in some hereditary myopathies (as Duchenne muscular dystrophy) and non-inherited/acquired myopathies (such as idiopathic inflammatory myopathies and fibromyalgia). These studies show that some myopathies are characterized by decreased concentration of plasma adiponectin and that hormone replenishment induces beneficial effects in the diseased muscles. Overall, these findings suggest that adiponectin could constitute a future new therapeutic approach for the improvement of the abnormalities caused by myopathies.
A Novel Network-Based Computational Model for Prediction of Potential LncRNA–Disease Association
Authors: Liu Yang , Feng Xiang , Zhao Haochen , Xuan Zhanwei , Wang Lei
Published: March 2019

Accumulating studies have shown that long non-coding RNAs (lncRNAs) are involved in many biological processes and play important roles in a variety of complex human diseases. Developing effective computational models to identify potential relationships between lncRNAs and diseases can not only help us understand disease mechanisms at the lncRNA molecular level, but also promote the diagnosis, treatment, prognosis, and prevention of human diseases. For this paper, a network-based model called NBLDA was proposed to discover potential lncRNA–disease associations, in which two novel lncRNA–disease weighted networks were constructed. They were first based on known lncRNA–disease associations and topological similarity of the lncRNA–disease association network, and then an lncRNA–lncRNA weighted matrix and a disease–disease weighted matrix were obtained based on a resource allocation strategy of unequal allocation and unbiased consistence. Finally, a label propagation algorithm was applied to predict associated lncRNAs for the investigated diseases. Moreover, in order to estimate the prediction performance of NBLDA, the framework of leave-one-out cross validation (LOOCV) was implemented on NBLDA, and simulation results showed that NBLDA can achieve reliable areas under the ROC curve (AUCs) of 0.8846, 0.8273, and 0.8075 in three known lncRNA–disease association datasets downloaded from the lncRNADisease database, respectively. Furthermore, in case studies of lung cancer, leukemia, and colorectal cancer, simulation results demonstrated that NBLDA can be a powerful tool for identifying potential lncRNA–disease associations as well.
Crop Pollen Development under Drought: From the Phenotype to the Mechanism
Authors: Yu Jing , Jiang Mengyuan , Guo Changkui
Published: March 2019

Drought stress induced pollen sterility is a harmful factor that reduces crop yield worldwide. During the reproductive process, the meiotic stage and the mitotic stage in anthers are both highly vulnerable to water deficiency. Drought at these stages causes pollen sterility by affecting the nature and structure of the anthers, including the degeneration of some meiocytes, disorientated microspores, an expanded middle layer and abnormal vacuolizated tapeta. The homeostasis of the internal environment is imbalanced in drought-treated anthers, involving the decreases of gibberellic acid (GA) and auxin, and the increases of abscisic acid (ABA), jasmonic acid (JA) and reactive oxygen species (ROS). Changes in carbohydrate availability, metabolism and distribution may be involved in the effects of drought stress at the reproductive stages. Here, we summarize the molecular regulatory mechanism of crop pollen development under drought stresses. The meiosis-related genes, sugar transporter genes, GA and ABA pathway genes and ROS-related genes may be altered in their expression in anthers to repair the drought-induced injures. It could also be that some drought-responsive genes, mainly expressed in the anther, regulate the expression of anther-related genes to improve both drought tolerance and anther development. A deepened understanding of the molecular regulatory mechanism of pollen development under stress will be beneficial for breeding drought-tolerant crops with high and stable yield under drought conditions.
Evaluation of the Biocompatibility of CS-Graphene Oxide Compounds In Vivo
Authors: López Tenorio Diego , Valencia Carlos H. , Valencia Cesar , Zuluaga Fabio , Valencia Mayra E. , Mina José H. , Grande Tovar Carlos David
Published: March 2019

In the last few years, graphene oxide (GO) has gained considerable importance in scaffold preparation for tissue engineering due to the presence of functional groups that allow the interaction between the extracellular matrix and the components of the cellular membrane. The interaction between GO and chitosan (CS) can not only improve the biomechanical properties of the scaffold but also generate a synergistic effect, facilitating tissue recovery. In vivo studies on GO are scarce; therefore, biocompatibility tests on CS-GO scaffolds and bone regeneration experiments on critical size defects were carried out on Wistar rats. Scaffolds made of CS, CS-GO 0.5%, and CS-GO 1% were prepared and implanted on Wistar rats cranial bones for three months. Scaffold samples were analyzed through histochemistry and scanning electron microscopy. The analysis performed showed reabsorption of the material by phagocytic activity and new bone formation. The CS-GO 0.5% formulation gave the best performance in bone regeneration, with excellent biocompatibility. These results show the potential of this compound for tissue regeneration opening and medical applications.
Spare Parts from Discarded Materials: Fetal Annexes in Regenerative Medicine
Authors: Gaggi Giulia , Izzicupo Pascal , Di Credico Andrea , Sancilio Silvia , Di Baldassarre Angela , Ghinassi Barbara
Published: March 2019

One of the main aims in regenerative medicine is to find stem cells that are easy to obtain and are safe and efficient in either an autologous or allogenic host when transplanted. This review provides an overview of the potential use of the fetal annexes in regenerative medicine: we described the formation of the annexes, their immunological features, the new advances in the phenotypical characterization of fetal annexes-derived stem cells, the progressions obtained in the analysis of both their differentiative potential and their secretoma, and finally, the potential use of decellularized fetal membranes. Normally discarded as medical waste, the umbilical cord and perinatal tissue not only represent a rich source of stem cells but can also be used as a scaffold for regenerative medicine, providing a suitable environment for the growth and differentiation of stem cells.
Physical Activity Alleviates Cognitive Dysfunction of Alzheimer’s Disease through Regulating the mTOR Signaling Pathway
Authors: Kou Xianjuan , Chen Dandan , Chen Ning
Published: March 2019

Alzheimer’s disease (AD) is one of the most common aging-related progressive neurodegenerative disorders, and can result in great suffering for a large portion of the aged population. Although the pathogenesis of AD is being elucidated, the exact mechanisms are still unclear, thereby impeding the development of effective drugs, supplements, and other interventional strategies for AD. In recent years, impaired autophagy associated with microRNA (miRNA) dysfunction has been reported to be involved in aging and aging-related neurodegenerative diseases. Therefore, miRNA-mediated regulation for the functional status of autophagy may become one of the potent interventional strategies for AD. Mounting evidence from in vivo AD models has demonstrated that physical activity can exert a neuroprotective role in AD. In addition, autophagy is strictly regulated by the mTOR signaling pathway. In this article, the regulation of the functional status of autophagy through the mTOR signaling pathway during physical activity is systematically discussed for the prevention and treatment of AD. This concept will be beneficial to developing novel and effective targets that can create a direct link between pharmacological intervention and AD in the future.
Electronic Effects of the Substituents on Relaxometric and CEST Behaviour of Ln(III)-DOTA-Tetraanilides
Authors: Lagostina Valeria , Leone Loredana , Carniato Fabio , Digilio Giuseppe , Tei Lorenzo , Botta Mauro
Published: March 2019

Three different 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetamide (DOTAM) derivatives bearing as amide N-substituents phenyl, p-methoxyphenyl and p-ethylbenzoate groups were synthesized and the 1H and 17O NMR relaxometric behaviour of the Gd(III)-chelates and chemical exchange saturation transfer (CEST) effect of the Eu(III) complexes were evaluated. The electronic properties of the substituents were shown to strongly influence the coordinated water exchange rate (kex), resulting in five times faster kex for the electron donating phenylmethoxy group compared to the electron withdrawing ethylbenzoate group.
Low-Temperature Ordering in the Cluster Compound (Bi8)Tl[AlCl4]3
Authors: Knies Maximilian , Kaiser Martin , Lê Anh Mai , Efimova Anastasia , Doert Thomas , Ruck Michael
Published: March 2019

The reaction of Bi, BiCl3, and TlCl in the ionic liquid [BMIm]Cl·4AlCl3 (BMIm = 1-n-butyl-3-methylimidazolium) at 180 °C yielded air-sensitive black crystals of (Bi8)Tl[AlCl4]3. X-ray diffraction on single crystals at room temperature revealed a structure containing [ Tl ( AlCl 4 ) 3 ] ∞ 1 2 − strands separated by isolated Bi82+ square antiprisms. The thallium(I) ion is coordinated by twelve Cl− ions of six [AlCl4]− groups, resulting in a chain of face-sharing [TlCl12]11− icosahedra. The Bi82+ polycation is disordered, simulating a threefold axis through its center and overall hexagonal symmetry (space group P63/m). Slowly cooling the crystals to 170 K resulted in increased order in the Bi8 cluster orientations. An ordered structure model in a supercell with a’ = 2a, b’ = 2b, c’ = 3c and the space group P65 was refined. The structure resembles a hexagonal perovskite, with complex groups in place of simple ions.