bluuebunny/crossref_metadata_2025 huggingface dataset:
Trophic Structure and Isotopic Niche of Invaded Benthic Communities on Tropical Rocky Shores
Authors: Pires-Teixeira Larissa M. , Neres-Lima Vinicius , Barbosa Plínio C. , Creed Joel C.
Published: December 2024

When a species is introduced in a new location, it is common for it to establish itself when it finds favorable conditions in the receptor community with regard to interspecific interactions with native species. The azooxanthellate corals Tubastraea coccinea and Tubastraea tagusensis are invasive species introduced in the Caribbean Sea, the Gulf of Mexico, and the Brazilian Southwest Atlantic. They are successful competitors for space, have multiple reproductive modes, and have high larval dispersion and recruitment, but studies on food and trophic relationships of the genus Tubastraea are still scarce. In the present study, we used isotopic values of δ13C and δ15N to investigate trophic relationships in rocky shore communities invaded by T. tagusensis and T. coccinea corals under different oceanographic and anthropogenic contexts. Using metrics derived from the isotopic values, we show that invaded communities have a lower degree of trophic diversity, with species characterized by similar trophic ecologies while abiotic factors seem to contribute to the biotic resistance of communities exposed to invasion events. Tubastraea spp. occupy a niche space similar to that occupied by the native community of suspension feeders, sharing resources already consumed by the receptor community, which makes invading corals successful competitors for food.
Nicotinamide Mononucleotide Alleviates Bile Acid Metabolism and Hormonal Dysregulation in Letrozole-Induced PCOS Mice
Authors: Ren Caifang , Zhang Shuang , Ma Jianyu , Huang Junjie , Huang Pan , Qu Mingzi , Zhao Haoyue , Zhou Zhengrong , Gong Aihua
Published: December 2024

Polycystic ovary syndrome (PCOS) involves complex genetic, metabolic, endocrine, and environmental factors. This study explores the effects of nicotinamide mononucleotide (NMN) in a letrozole-induced PCOS mouse model, focusing on metabolic regulation. Letrozole-induced aromatase inhibition elevated androgen and reduced bile acid levels, linking liver dysfunction and gut imbalance to PCOS. Letrozole-treated mice exhibited disrupted estrous cycles, ovarian congestion, and elevated testosterone. NMN intervention alleviated hyperandrogenism, ovarian abnormalities, and bile acid decline but did not fully restore the estrous cycle or improve lipid profiles. Metabolomic analysis showed that NMN partially reversed bile acid and lipid metabolism disturbances. These findings highlight NMN’s protective role in reducing hyperandrogenism and ovarian cyst formation. However, effective PCOS treatment should target liver and gut metabolism, not just ovarian symptoms, to mitigate systemic effects. Bile acid dysregulation may play a key role in PCOS progression and warrants further investigation.
Characteristic Muscle Quality Parameters of Male Largemouth Bass (Micropterus salmoides) Distinguished from Female and Physiological Variations Revealed by Transcriptome Profiling
Authors: Wang Qingchun , Lu Siqi , Tao Yifan , Hua Jixiang , Zhuge Yan , Chen Wenhua , Qiang Jun
Published: December 2024

Male largemouth bass (Micropterus salmoides) are often overlooked because females grow faster. We explored the value of male largemouth bass by comparing muscle nutrition, texture, and transcriptomes between males and females. Females grew faster than males (p < 0.05) because of lipid accumulation. Male fish muscles had higher contents of serine, valine, methionine, arginine, nervonic acid, and α-linolenic acid (p < 0.05), and female fish muscles had higher contents of aspartic acid, glycine, cysteine, leucine, palmitic acid, docosahexaenoic acid, and 11,14,17-eicosatrienoic acid (p < 0.05). Male muscles had a higher concentration of collagen fibers and greater shear force, indicative of a chewier texture. Male muscles had a lighter color, suggesting that they were less susceptible to oxidation and deterioration. Transcriptomic analyses revealed upregulation of lpl, sadb, dgat2, bhmt, tecrb, and hsd3b7, encoding components of amino acid and fatty acid metabolism; and upregulation of akt2, src, and kras, encoding crucial regulators of cellular immunity and homeostasis, in male muscles. Immunity-related pathways, including apoptosis, ErbB signaling, and cellular senescence, were enriched in male fish muscles, indicating heightened immune function. The muscles of male fish have a unique profile and distinctive advantages in terms of nutrition, flavor, texture, and transcriptional regulation.
Lysyl Oxidase Mediates Proliferation and Differentiation in the Esophageal Epithelium
Authors: Kennedy Kanak V. , Wang Joshua X. , McMillan Emily , Zhou Yusen , Teranishi Ryugo , Semeao Ann , Mirchandani Leena , Umeweni Chizoba N. , Dhakal Diya , Baccarella Alyssa , Ishikawa Satoshi , Sasaki Masaru , Itami Takefumi , Harman Adele C. , Joannas Leonel , Karakasheva Tatiana A. , Nakagawa Hiroshi , Muir Amanda B.
Published: December 2024

In homeostatic conditions, the basal progenitor cells of the esophagus differentiate into a stratified squamous epithelium. However, in the setting of acid exposure or inflammation, there is a marked failure of basal cell differentiation, leading to basal cell hyperplasia. We have previously shown that lysyl oxidase (LOX), a collagen crosslinking enzyme, is upregulated in the setting of allergic inflammation of the esophagus; however, its role beyond collagen crosslinking is unknown. Herein, we propose a non-canonical epithelial-specific role of LOX in the maintenance of epithelial homeostasis using 3D organoid and murine models. We performed quantitative reverse transcriptase PCR, Western blot, histologic analysis, and RNA sequencing on immortalized non-transformed human esophageal epithelial cells (EPC2-hTERT) with short-hairpin RNA (shRNA) targeting LOX mRNA in both monolayer and 3D organoid culture. A novel murine model with a tamoxifen-induced Lox knockout specific to the stratified epithelium (K5CreER; Loxfl/fl) was utilized to further define the role of epithelial LOX in vivo. We found that LOX knockdown decreased the proliferative capacity of the esophageal epithelial cells in monolayer culture, and dramatically reduced the organoid formation rate (OFR) in the shLOX organoids. LOX knockdown was associated with decreased expression of the differentiation markers filaggrin, loricrin, and involucrin, with RNA sequencing analysis revealing 1224 differentially expressed genes demonstrating downregulation of pathways involved in cell differentiation and epithelial development. Mice with Lox knockout in their stratified epithelium demonstrated increased basaloid content of their esophageal epithelium and decreased Ki-67 staining compared to the vehicle-treated mice, suggesting reduced differentiation and proliferation in the Lox-deficient epithelium in vivo. Our results demonstrate, both in vivo and in vitro, that LOX may regulate epithelial homeostasis in the esophagus through the modulation of epithelial proliferation and differentiation. Understanding the mechanisms of perturbation in epithelial proliferation and differentiation in an inflamed esophagus could lead to the development of novel treatments that could promote epithelial healing and restore homeostasis.
Sample Preparation for Metabolomic Analysis in Exercise Physiology
Authors: Nakhod Valeriya I. , Butkova Tatiana V. , Malsagova Kristina A. , Petrovskiy Denis V. , Izotov Alexander A. , Nikolsky Kirill S. , Kaysheva Anna L.
Published: December 2024

Metabolomics investigates final and intermediate metabolic products in cells. Assessment of the human metabolome relies principally on the analysis of blood, urine, saliva, sweat, and feces. Tissue biopsy is employed less frequently. Understanding the metabolite composition of biosamples from athletes can significantly improve our knowledge of molecular processes associated with the efficiency of training and recovery. Such knowledge may also lead to new management opportunities. Successful execution of metabolomic studies requires simultaneous qualitative and quantitative analyses of numerous small biomolecules in samples under test. Unlike genomics and proteomics, which do not allow for direct assessment of enzymatic activity, metabolomics focuses on biochemical phenotypes, providing unique information about health and physiological features. Crucial factors in ensuring the efficacy of metabolomic analysis are the meticulous selection and pre-treatment of samples.
Sustainable Lipase Immobilization: Chokeberry and Apple Waste as Carriers
Authors: Jasińska Karina , Nowosad Maksym , Perzyna Aleksander , Bielacki Andrzej , Dziwiński Stanisław , Zieniuk Bartłomiej , Fabiszewska Agata
Published: December 2024

In the modern world, the principles of the bioeconomy are becoming increasingly important. Recycling and reusability play a crucial role in sustainable development. Green chemistry is based on enzymes, but immobilized biocatalysts are still often designed with synthetic polymers. Insoluble carriers for immobilized biocatalysts, particularly those derived from agro-industrial waste such as mesoporous lignocellulosic materials, offer a promising alternative. By using waste materials as support for enzymes, we can reduce the environmental impact of waste disposal and contribute to the development of efficient bioprocessing technologies. The current study aimed to assess the possibility of using apple and chokeberry pomace as carriers for the immobilization of Palatase 20000L (lipase from Rhizomucor miehei). The analysis of lignocellulosic materials revealed that chokeberry pomace has a higher neutral detergent fiber (NDF) and lignin contents than apple pomace. Moreover, Scanning Electron Microscopy (SEM) observations indicated similar compact structures in both pomaces. The lipase activity assays demonstrated that immobilization of lipase from R. miehei onto apple and chokeberry pomace improves their properties, especially the synthetic activity. The findings highlight the potential of utilizing fruit pomaces not only as a source of bioactive compounds but also in enhancing enzyme stability for industrial applications.
Disruption of Sex-Linked Sox3 Causes ZW Female-to-Male Sex Reversal in the Japanese Frog Glandirana rugosa
Authors: Miura Ikuo , Hasegawa Yoshinori , Ito Michihiko , Ezaz Tariq , Ogata Mitsuaki
Published: December 2024

Sox3 is an ancestral homologous gene of the male-determining Sry in eutherian mammals and determines maleness in medaka fish. In the Japanese frog, Glandirana rugosa, Sox3 is located on the Z and W chromosomes. To assess the sex-determining function of Sox3 in this frog, we investigated its expression in gonads during early tadpole development and conducted genome-editing experiments. We found that the Sox3 mRNA levels in the gonads/mesonephroi were much higher in ZW females than that in ZZ males, and that the W-borne allele was dominantly expressed. A higher expression in ZW females preceded the onset of the sexually dimorphic expression of other autosomal sex differentiation genes. The Sox3 protein was detected by immunostaining in the somatic cells of early tadpole gonads around the boundary between the medulla and cortex in ZW females, whereas it was outside the gonads in ZZ males. Disrupting Sox3 using TALEN, which targets two distinct sites, generated sex-reversed ZW males and hermaphrodites, whereas no sex reversal was observed in ZZ males. These results suggest that the sex-linked Sox3 is involved in female determination in the ZZ-ZW sex-determining system of the frog, an exact opposite function to the male determination of medaka Sox3y and eutherian Sry.
Nano-Delivery Revolution: Harnessing Mesenchymal Stem Cell-Derived Exosomes’ Potential for Wound Healing
Authors: Raghav Pawan Kumar , Mann Zoya
Published: December 2024

Stem cell transplantation has proven effective in treating acute and chronic wounds, but its limitations, such as low cellular viability and the need for specialized transportation, highlight the necessity for alternative approaches. This review explores the potential of engineered exosomes, containing identified miRNAs/peptides, as a more stable and efficient cell-free therapy for regenerative medicine, particularly in wound healing. The discussion emphasizes the benefits of exosomes, including their stability, reduced damage, and consistent biological activity, paving the way for innovative applications like lyophilized exosomes, mist spray delivery, and exosome-based scaffolds. The exploration of cell-free therapy in this review holds promising implications for advancing wound-healing strategies.
Therapeutic Approach to Primary Tic Disorders and Associated Psychiatric Comorbidities
Authors: Berzosa-Gonzalez Irene , Martinez-Horta Saul , Pérez-Pérez Jesus , Kulisevsky Jaime , Pagonabarraga Javier
Published: December 2024

Background/Objectives: The treatment of tics and psychiatric comorbidities is crucial when they affect the patient’s well-being and relationships. However, the optimal pharmacological treatment (PT) tailored to each patient’s phenotype remains unclear. The primary objective of this study is to describe the clinical characteristics and treatment received for tics and psychiatric comorbidities in our cohort of children and adult patients with tic disorders. Additionally, a further aim was to quantify the severity of tics, comorbidities and overall severity, and the overall clinical changes observed during the follow-up. Methods: Retrospective descriptive study of patients with tic disorders under follow-up at our Tic Functional Unit from January 2022 to March 2024. Two independent neurologists retrospectively applied the Clinical Global Impression of Change (CGI-C) and the Clinical Global Impression of Severity (CGI-S) scales at baseline and at last assessment. Results: A total of 36 individuals were included (63.8% males, median age = 18 years, IQR 19): 94.4% with Tourette syndrome (TS), 2.8% with chronic tic disorder (CTD), and 2.8% with provisional tic disorder (PTD). A total of 86% had at least one psychiatric comorbidity, the most common being obsessive–compulsive symptomatology (OCS) (52%), anxiety (52%), and attention deficit hyperactivity disorder (ADHD) (35%). At last assessment, 26 patients (72.2%) were on undergoing PT for tics and 3 were receiving additional botulinum toxin. The most used medication for tics were aripiprazole (46.2%) and clonazepam (46.2%), and for psychiatric comorbidities, SSRIs (42.9%), methylphenidate (19%), and benzodiazepines (57.1%). Overall improvement according to the CGI-C scale was mild (CGI-C 3). Children and adolescents showed greater improvement than adults (CGI-C 2 vs. 3; p = 0.005). Aripiprazole and clonazepam produced similar outcomes in reducing CGI-C. Conclusions: We observed a favorable clinical course in patients treated with aripiprazole and clonazepam, which appear to be better than that obtained with other treatments. We consider that clonazepam may be useful as a first-line monotherapy and as an adjuvant for both tics and comorbidities in selected cases.
Psychiatric Disorders and Cognitive Fluctuations in Parkinson’s Disease: Changing Approaches in the First Decades of the 21st Century
Authors: Onofrj Marco , De Rosa Matteo Alessandro , Russo Mirella , Ajdinaj Paola , Calisi Dario , Thomas Astrid , Sensi Stefano Luca
Published: December 2024

Parkinson’s Disease (PD) is a multifaceted neurodegenerative disorder characterized, in addition to the well-recognized motor disturbances, by a complex interplay between cognitive and psychiatric manifestations. We dissect the complex landscape of PD-related psychiatric symptoms, taking into account the impact of functional neurological disorders, somatic delusions, impulse control disorders, and conditions within the bipolar spectrum. The newer entities of somatoform and functional neurological disorders, as well as preexisting bipolar spectrum disorders, are analyzed in detail. Moreover, we emphasize the need for a holistic understanding of PD, wherein the cognitive and psychiatric dimensions are valued alongside motor symptoms. Such an approach aims to facilitate early detection and personalized interventions, and enhance the overall quality of life for individuals suffering from this neurodegenerative disorder.
Advancing Neuropsychological Rehabilitation in Primary Progressive Aphasia Based on Principles of Cognitive Neuroscience: A Scoping Review and Systematic Analysis of the Data
Authors: Gkintoni Evgenia , Michou Emilia
Published: December 2024

Background/Objectives: This systematic review of neuropsychological rehabilitation strategies for primary progressive aphasia will consider recent developments in cognitive neuroscience, especially neuroimaging techniques such as EEG and fMRI, to outline how these tools might be integrated into clinical practice to maximize treatment outcomes. Methods: A systematic search of peer-reviewed literature from the last decade was performed following the PRISMA guidelines across multiple databases. A total of 63 studies were included, guided by predefined inclusion and exclusion criteria, with a focus on cognitive and language rehabilitation in PPA, interventions guided by neuroimaging, and mechanisms of neuroplasticity. Results: Integration of neuroimaging techniques contributes to the increase in the efficacy of interventions with critical information about the neural mechanisms underlying language deficits in the aphasias. Traditional rehabilitation strategies, technology-assisted interventions, and non-invasive brain stimulation techniques hold considerable promise for language improvement. Neuroimaging was also found to be necessary in subtype-specific differentiation toward tailoring therapeutic intervention. Evidence also shows that directed and sustained interventions using neuroplasticity can have long-term effects in managing the symptoms of PPA. Conclusions: The present review underlines the necessity of including cognitive neuroscience techniques within neuropsychological rehabilitation to enhance therapeutic outcomes in PPA. In addition, neuroimaging modalities such as EEG and fMRI are also of great importance in understanding the underlying neurobiology of language disturbances and guiding tailored interventions. Long-term benefits of these approaches should be evaluated, including their applicability in routine clinical practice.
Interoceptive Awareness and Female Orgasm Frequency and Satisfaction
Authors: Dixon Emily , Poerio Giulia L. , Rieger Gerulf , Klabunde Megan
Published: December 2024

Background: The female orgasm is a highly understudied phenomenon that is linked to both wellbeing and relationship satisfaction in women. Although orgasm has been associated with interoception—the sense of the physiological condition of the body—very few studies have directly examined the influence that interoception has on orgasm. Objectives: This study investigates how the subjective experience of one’s interoceptive capacities (called interoceptive awareness) is associated with self-reported orgasm frequency and satisfaction in people who identify as women. Methods: In a dataset of 318 women, orgasm frequency and satisfaction were both rated significantly higher for solitary as compared to partnered sexual experiences. Results: Analysis of how dimensions of interoceptive awareness correlated with orgasm frequency and satisfaction showed that (1) ‘Noticing’ predicted orgasm frequency (but not satisfaction) across both solitary and partnered interactions, (2) ‘Attention Regulation’ predicted greater frequency and satisfaction of solitary orgasm (but not partnered interactions), and (3) ‘Body Trusting’ predicted orgasm satisfaction (but not frequency) across both solitary and partnered contexts. Conclusions: Findings underscore the importance of moving beyond orgasmic dysfunction research by investigating how interoception is associated with healthy—and potentially even optimal—orgasmic functioning in women.
Psychological Treatments for Alexithymia: A Systematic Review
Authors: Tsubaki Kanako , Shimizu Eiji
Published: December 2024

Alexithymia, a psychological condition characterized by emotional suppression, is positively correlated with depression and anxiety and can develop into various mental disorders. Although alexithymia affects 10% of the symptomatic population and 25% of psychiatric patients, there has been a paucity of intervention studies. Even though several effective psychological treatments, including cognitive behavioral therapy (CBT), have been tested in recent years, there is a lack of comprehensive reviews on their efficacy. The objectives of this systematic review were to explore and synthesize findings from recent randomized controlled trials (RCTs) about psychological treatments, with the following inclusion criteria: (1) published from 2010 to 2024; (2) full text being available in English; (3) peer-reviewed journals; and (4) baselines and outcomes measured by TAS-20 and raw data were provided. We excluded non-psychological studies and studies involving mindfulness and DBT. We searched electronic databases (PubMed, PsycInfo, and Google Scholar) and found 18 RCTs and 21 arms for alexithymia, with a combined total of 1251 participants. Fourteen arms (67%) investigated the effect of CBT on alexithymia, including acceptance and commitment therapy (seven arms), behavioral activation therapy (two arms), schema therapy, and compassion-focused therapy. The results indicated that most psychological interventions significantly decreased TAS-20, illustrating a showcase of treatments from each trial with different effect sizes (within-intervention group, ranging from 0.41 to 13.25). However, due to the heterogeneity between the studies, this review study may not be conclusive enough to make each intervention standardized. Further high-quality RCTs with larger sample sizes and more consistent methodologies are needed, and corrective findings from such studies should be applied to produce more robust evidence-based psychological interventions for treating alexithymia.
Neuromodulation Strategies in Lifelong Bipolar Disorder: A Narrative Review
Authors: Bernabei Laura , Leone Beniamino , Hirsch Daniele , Mentuccia Valentina , Panzera Alessia , Riggio Francesco , Sangiovanni Loredana , Piserchia Valentina , Nicolò Giuseppe , Pompili Enrico
Published: December 2024

Bipolar disorder is a debilitating psychiatric condition characterized by recurrent episodes of mania and depression, affecting millions worldwide. While pharmacotherapy remains the cornerstone of treatment, a significant proportion of patients exhibit inadequate response or intolerable side effects to conventional medications. In recent years, neuromodulation techniques have emerged as promising adjunctive or alternative treatments for bipolar disorder. We performed a narrative review, according to the Scale for the Assessment of Narrative Review Articles (SANRA) guidelines, to provide a comprehensive overview of the current literature on neuromodulation interventions in bipolar disorder across the course of lifespan. Specifically, it examines the efficacy, safety, and mechanisms of action of various neuromodulation strategies, including, among others, transcranial magnetic stimulation (TMS), electroconvulsive therapy (ECT), vagus nerve stimulation (VNS), deep brain stimulation (DBS), and it describes the therapeutic experiences across the different ages of illness. Additionally, this review discusses the clinical implications, challenges, and future directions of the integration, in clinical practice, of neuromodulation into the management of bipolar disorder. By synthesizing evidence from different studies, this review aims to inform clinicians, researchers, and stakeholders about the evolving landscape of neuromodulation treatments and their potential role in improving outcomes for individuals with bipolar disorder.
Design Optimization of Energy-Efficient Residential Buildings in Morocco
Authors: Boumlik Karim , Belarbi Rafik , Ahachad Mohammed , Mahdaoui Mustapha , Radoine Hassan , Krarti Moncef
Published: December 2024

In this paper, an optimization-based analysis approach is presented to cost-effectively improve the energy efficiency of residential buildings in Morocco. This study introduces a unique focus on the Moroccan context, where a comprehensive application of energy efficiency optimization has not yet been undertaken. This analysis considers the interactive effects among various energy efficiency measures to determine optimal combinations for designing high-energy performance, as well as net-zero energy, residential buildings for six climate zones in Morocco. In particular, the design analysis approach combines a whole-building simulation with the sequential search technique, providing a novel, integrated cost–benefit analysis that minimizes lifecycle costs (LCC) while maximizing energy savings for each climate zone. This study also includes an unprecedented comparison of optimized designs, reference designs, and current Moroccan building regulations (RTCM), highlighting potential improvements to the existing regulatory framework. While the sequential search method has been applied elsewhere, its specific application to achieve net-zero energy homes in the Moroccan context with comparable LCC is a new contribution. The analysis results show that houses in Morocco can be cost-effectively designed to achieve annual energy savings of 51% for Zone 1, 53% for Zone 2, 60% for Zone 3, 67% for Zone 4, 54% for Zone 5, and 56% for Zone 6 compared to the current construction practices considered as reference designs. Moreover, the results indicate that houses can reach net-zero energy building designs with almost the same LCC as the reference design cases for all the climate zones in Morocco.
Quantifying the Self-Healing Efficiency of Bioconcrete Using Bacillus subtilis Immobilized in Polymer-Coated Lightweight Expanded Clay Aggregates
Authors: Ahmad Izhar , Shokouhian Mehdi , Jenkins Marshell , McLemore Gabrielle L.
Published: December 2024

Concrete is prone to cracking over time, leading to the deterioration of concrete structures. Using the biomineralization capabilities of bacteria, cracks in concrete can be remediated in favorable conditions. In this study, Bacillus subtilis spores were immobilized in three different healing agents, namely lightweight expanded clay aggregates (LECAs), polyvinyl acetate (PVA) fibers, and an air-entraining admixture (AEA). Bacillus subtilis spores, with a turbidity equivalent to a 4 McFarland standard, were used in three different dosages, namely 0.01, 0.1, and 1% (by weight) of cement. Based on the dosage, three groups were developed and each group consisted of a total of nine mixes, which were differentiated based on the method of delivery of the bacterial spores. The specimens were pre-cracked after 7 days, using an embedded steel rod, after being post-tensioned in a universal testing machine. The self-healing efficiency of the concrete was evaluated using ultrasonic pulse velocity testing and surface crack analysis, using ImageJ software, and the self-healing precipitate was analyzed using microstructural tests, namely scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy analysis. The results verified that the self-healing efficiency of the concrete improved with the increase in the bacterial dosage and with an increase in the curing time. LECAs proved to be a promising bacterial carrier, by accommodating the spores and nutrient media over a period of 196 days. PVA fibers helped in bridging the cracks and provided nucleation sites for the bacteria, which enhanced the calcite precipitation. Similarly, the AEA also improved crack healing by encapsulating the spores and sealing cracks up to 0.25 mm, when used in conjunction with LECAs. Furthermore, microstructural tests verified the formation of calcite as a healing product within the cracks in the bioconcrete. The results of this study offer valuable insights for the construction industry, highlighting the ability of bacteria to reduce the deterioration of concrete structures and promoting a sustainable approach that minimizes the need for manual repairs, particularly in hard-to-reach areas.
Impact of Microwave Pre-Curing on Pore Structure and Environmental Performance of Metakaolin- and Fly Ash-Based Geopolymers
Authors: Dong Yanhui , El-Naggar Mohamed R. , Gao Runhui , Li Yefan , Zhao Yixin
Published: December 2024

Microwave technology in geopolymer synthesis offers a transformative, sustainable alternative to traditional methods, enhancing material properties and production efficiency. However, the effects of microwave-induced changes on pore structure and their relationship with mechanical strength and environmental performance, such as heavy metal leachability, are not fully understood. This study investigates the impact of microwave pre-curing on geopolymers, focusing on how microwave power and duration influence their pore structure and environmental performance. A total of 48 mixtures were prepared using sodium silicate and sodium hydroxide as alkali activators, with metakaolin and fly ash as raw materials. The modulus was adjusted to 1.5, and the liquid-to-solid ratio was set at 1.6 for metakaolin and 0.7 for fly ash. Microwave irradiation power settings of 100 W, 300 W, 440 W, 600 W, and 800 W were tested. The heating times ranged from 30 s to 90 s at intervals of 15 s. Our findings reveal that optimal microwave settings (100 watts for 45 s) can significantly enhance mechanical properties, with compressive strengths reaching 15.9 MPa for fly ash-based and 9.094 MPa for metakaolin-based geopolymers. However, excessive microwave energy leads to increased porosity, with adverse effects on structural integrity. Moreover, microwave pre-curing effectively reduces heavy metal leachability. Chromium (III) was used in leaching tests and it was demonstrated that ion concentrations as low as 0.097 mg/L enhance environmental safety. Advanced techniques like Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and X-ray CT were applied for the analysis of the atomic bonding, phases and pore structure of the studied geopolymers along with their ability to withstand compression (MPa). Chromium (III) was encapsulated and its leached concentration was measured by ICP-MS to evaluate the performance of the synthesized geopolymers. These results underscore the need for precise control over microwave irradiation parameters to maximize the benefits while mitigating negative impacts. This study provides valuable insights into the controlled use of microwave technology for geopolymer synthesis, recommending optimal irradiation conditions for improved performance and sustainability and advancing sustainable construction materials. The developed geopolymers show promise for applications in construction, waste stabilization, and heavy metal immobilization, contributing to more sustainable and environmentally friendly materials in these industries.
LCA-TOPSIS Integration for Minimizing Material Waste in the Construction Sector: A BIM-Based Decision-Making
Authors: Yardimci Yigit , Kurucay Emre
Published: December 2024

The construction sector is one of the industries with the highest environmental impact due to resource consumption and waste generation. Material waste exacerbates these impacts by increasing carbon emissions and energy consumption. This study introduces an innovative approach by integrating Life Cycle Assessment (LCA) and the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) to evaluate material waste and environmental impacts simultaneously. By analyzing scenarios of material use in the design and construction phases, this study explores their effects on material efficiency and environmental performance while addressing a notable research gap. Existing studies on the integration of LCA and TOPSIS in evaluating material waste and its environmental impacts remain limited. This research not only demonstrates the applicability of these methods but also contributes to filling this gap. Material waste and efficiency were assessed through Building Information Modeling (BIM), while BIM-LCA integration was used to evaluate environmental impacts. The findings were examined in two stages: LCA and TOPSIS. The TOPSIS analysis considered two scenarios—material waste and environmental impacts. In the first scenario, cast-in-place concrete (5000 psi) and stone and ceramic tiles emerged as priorities. In the second scenario, where carbon emissions and environmental impacts were emphasized, cast-in-place concrete (5000 psi), laminated timber, and stone tiles were identified as critical materials. The results reveal that reducing material waste significantly enhances environmental performance, lowers costs, and promotes sustainability. These findings provide practical insights for developing sustainable strategies in diverse cultural and geographical contexts, particularly for residential projects. The integration of LCA and TOPSIS offers a robust decision-making framework, enabling targeted actions to minimize environmental footprints across all life cycle stages. This study contributes to the literature by providing actionable recommendations for optimizing resource use and improving sustainability in construction practices.
Seismic Behavior Analysis of a 14th Century Anatolian Seljuk Kumbet
Authors: Colak Demirel Burcu Buram , Yardimci Yigit Can , Kurucay Hayrun Nisa
Published: December 2024

The preservation of cultural heritage and the seismic resilience of historic buildings are crucial for maintaining social identity, particularly in earthquake-prone regions. This study focuses on the modeling of Sırçalı Kumbet, a Seljuk monument built in the 14th century in Kayseri province, located in the Central Anatolia region of Turkey, using survey drawings and analysis using the finite element method (FEM) to evaluate its seismic performance. The analysis indicates that linear elastic calculation methods can serve as an initial approach for evaluating such geometrically complex structures. The findings demonstrate that Sırçalı Kumbet exhibits substantial structural rigidity, reducing deformation and enhancing resistance to material fatigue during seismic events. Displacement and stress analyses under G+EQx and G+EQy loading conditions reveal that tensile and compressive stresses remain within acceptable limits, with localized exceedances occurring at specific points, such as cavity corners and wall bases. While these localized stresses are manageable, they highlight areas that require continuous monitoring and potential reinforcement to ensure long-term stability. Additionally, the study suggests that the integration of regular maintenance and targeted reinforcement measures can further improve the monument’s durability and minimize potential damage. This research underscores the essential role of the FEM in bridging the gap between cultural heritage conservation and seismic resilience. It provides a methodological framework for integrating architectural, restoration, and engineering expertise into comprehensive conservation strategies. Future studies should expand this approach to include various building types and material properties to enhance the development of preservation strategies.
Research on the Simplified Calculation Model and Parameter Analysis of Large-Size PBL-Stiffened Steel–Concrete Joints
Authors: Liu Haolin , Du Baisong , Zhou Heying
Published: December 2024

To investigate the design principles and simplified calculation model of large-size PBL-stiffened steel–concrete joints, this study uses a Y-shaped rigid frame-tied arch composite bridge as an engineering background. Based on deformation coordination theory, a combination of theoretical analysis and numerical simulation was employed to derive a simplified calculation model that accounts for boundary conditions such as the stiffness of steel beam end restraints and the local bearing effect of the bearing plate. Parametric analysis of the steel–concrete joint was conducted. The results indicate that the derived simplified calculation model exhibits good accuracy and is suitable for calculating force transfer in various components of the steel–concrete joint under different boundary conditions. Using the simplified model, the effects of parameters such as steel–concrete joint length, connector stiffness, and structural axial stiffness on the axial force transfer in primary force-bearing components (connectors and bearing plates) were studied. The findings reveal that an excessively long steel–concrete joint does not effectively reduce maximum shear force; variations in connector stiffness primarily affect connectors farther from the bearing plate without changing the shear force distribution. Increasing the axial stiffness of the steel structure within a certain range can improve the maximum shear force in connectors, whereas increasing the axial stiffness of the concrete structure has the opposite effect.
A Review of Smart Camera Sensor Placement in Construction
Authors: Tian Wei , Li Hao , Zhu Hao , Wang Yongwei , Liu Xianda , Yang Rongzheng , Xie Yujun , Zhang Meng , Zhu Jun , Wang Xiangyu
Published: December 2024

Cameras, with their low cost and efficiency, are widely used in construction management and structural health monitoring. However, existing reviews on camera sensor placement (CSP) are outdated due to rapid technological advancements. Furthermore, the construction industry poses unique challenges for CSP implementation due to its scale, complexity, and dynamic nature. Previous reviews have not specifically addressed these industry-specific demands. This study aims to fill this gap by analyzing articles from the Web of Science and ASCE databases that focus exclusively on CSP in construction. A rigorous selection process ensures the relevance and quality of the included studies. This comprehensive review navigates through the complexities of camera and environment models, advocating for advanced optimization techniques like genetic algorithms, greedy algorithms, Swarm Intelligence, and Markov Chain Monte Carlo to refine CSP strategies. Simultaneously, Building Information Modeling is employed to consider the progress of construction and visualize optimized layouts, improving the effect of CSP. This paper delves into perspective distortion, the field of view considerations, and the occlusion impacts, proposing a unified framework that bridges practical execution with the theory of optimal CSP. Furthermore, the roadmap for future exploration in the CSP of construction is proposed. This work enriches the study of construction CSP, charting a course for future inquiry, and emphasizes the need for adaptable and technologically congruent CSP approaches amid evolving application landscapes.
Tolerability and Preliminary Outcomes of Adjuvant T-DM1 in HER2-Positive Breast Cancer After Neoadjuvant Therapy: The ATD Study
Authors: Krasniqi Eriseld , Filomeno Lorena , Arcuri Teresa , Di Lisa Francesca Sofia , Astone Antonio , Cutigni Claudia , Foglietta Jennifer , Nunzi Martina , Rossi Rosalinda , Minelli Mauro , Meattini Icro , Visani Luca , Scialino Jacopo , Livi Lorenzo , Moscetti Luca , Marchetti Paolo , Botticelli Andrea , Paris Ida , Pavese Francesco , D’Angelo Tatiana , Sini Valentina , Stani Simonetta , Valerio Maria Rosaria , Grassadonia Antonino , Tinari Nicola , Mazzotta Marco , Vergati Matteo , D’Auria Giuliana , Gamucci Teresa , D’Onofrio Loretta , Gasparro Simona , Roselli Arianna , Fulvi Alberto , Ferretti Gianluigi , Torchia Andrea , Giordano Monica , Greco Filippo , Pantano Francesco , Tonini Giuseppe , Fabbri Agnese , Bria Emilio , Garufi Giovanna , Fiorio Elena , Raffaele Mimma , Pistelli Mirco , Berardi Rossana , Saltarelli Rosa , Kayal Ramy , Ferranti Francesca Romana , Cannita Katia , Irelli Azzurra , D’Ostilio Nicola , De Rossi Costanza , Palumbo Raffaella , Cariello Anna , Sanguineti Giuseppe , Calabrò Fabio , Pizzuti Laura , Barba Maddalena , Botti Claudio , Pelle Fabio , Cappelli Sonia , Cavicchi Flavia , Puccica Ilaria , Villanucci Amedeo , Sperduti Isabella , Ciliberto Gennaro , Vici Patrizia
Published: December 2024

Background/Objectives: HER2-positive breast cancer (HER2+BC) is an aggressive subtype, with neoadjuvant treatment (NAT) aiming to achieve a pathological complete response (pCR) to improve long-term outcomes. Trastuzumab emtansine (T-DM1) has been established as the standard of care in the adjuvant setting for HER2+BC patients who do not obtain pCR. The ATD study aimed to evaluate the real-world tolerability of T-DM1 in this setting. The secondary objective was to assess the effectiveness. Methods: This was a multicenter, retrospective study across 24 Italian oncology centers, including 410 patients with HER2+BC treated with adjuvant T-DM1 following a lack of pCR after NAT. Patient characteristics, NAT regimens, and surgical outcomes were recorded. Tolerability was assessed by documenting adverse events (AEs) according to the CTCAE (v5.0). Preliminary effectiveness was evaluated in terms of relapse-free survival (RFS) and overall survival (OS). Results: Overall, 228 patients (55.6%) experienced at least one AE related to T-DM1, with 4.9% experiencing grade 3 or higher AEs. The most common AEs were hepatotoxicity (18.5%) and thrombocytopenia (17.6%). T-DM1 was discontinued in 10.0% of patients due to toxicity. After a median follow-up of 25 months, 31 relapse events (7.6%) and 22 deaths (5.4%) were reported. The preliminary incidence of RFS and OS events was similar between patients who completed the T-DM1 course and those who discontinued it early. Conclusions: T-DM1 demonstrated a manageable safety profile, and the adverse events were consistent with those reported in randomized trials. The data are not yet sufficient to allow for a formal analysis of RFS and OS, and long-term follow-up is required.
Shaping the Future of Myeloproliferative Neoplasm Therapy: Immune-Based Strategies and Targeted Innovations
Authors: Carturan Alberto , Morè Sonia , Poloni Antonella , Rupoli Serena , Morsia Erika
Published: December 2024

Numerous cutting-edge immunotherapy approaches have been developed for hematological malignancies, such as immune-checkpoint inhibitors for lymphomas, chimeric antigen receptor (CAR)-T-cell treatments for B-cell cancers, and monoclonal antibody therapies for acute myeloid leukemia (AML). However, achieving similar breakthroughs in MPNs has proven challenging. The key obstacles include the absence of universally expressed and MPN-specific surface markers, significant cellular and molecular variability among both individual patients and across different MPN subtypes, and the failure of treatments to stimulate an anti-tumor immune response due to the immune system disruptions caused by the myeloid neoplasm. Currently, there are several innovative therapies in clinical trials for MPNs. These include new JAK inhibitors with greater specificity for JAK2, as well as “add-on” medications designed to enhance the effectiveness of ruxolitinib, in both patients who are new to the drug and in those who have shown suboptimal responses. Additionally, there is ongoing exploration of novel therapeutic targets. In this review, we will explore the immunotherapy approaches that are currently used in clinical practice for MPNs, as well as emerging strategies that are likely to change the treatment of these diseases in the coming years.
Boosted Photoelectrochemical Water Oxidation Performance with a Quaternary Heterostructure: CoFe2O4/MWCNT-Doped MIL-100(Fe)/TiO2
Authors: Rehman Waheed , Saeed Faiq , Zhao Yong , Maryam Bushra , Arain Samia , Ayaz Muhammad , Jamil Asad , Liu Xianhua
Published: December 2024

Cobalt ferrite (CoFe2O4) combined with multi-walled carbon nanotubes (MWCNTs) is an outstanding material regarding photoelectrochemical water oxidation (PEC-WO) because of its excellent catalytic properties and stability. On the other hand, surface imperfections in CoFe2O4 can cause band bending and surface Fermi level pinning, significantly reducing its PEC conversion efficiency. Heterostructure engineering is essential for achieving increased light-gathering capacity and charge separation efficiency for PEC-WO. In this study, a quaternary heterostructure of CoFe2O4/MWCNT-doped Metal–Organic Framework-100 (Iron), MIL-100(Fe)/Titanium Oxide (TiO2) was synthesized by using a combination of hydrothermal, solvothermal, and “dip and dry” techniques. Characterization results confirmed the formation of a structural network of MIL-100(Fe) on TiO2 surfaces, enhanced by the incorporation of MWCNTs during the hydrothermal reaction. Under 1 sun irradiation, the resultant quaternary heterostructure displayed a photocurrent density (Jph) of 3.70 mA cm−2 under free bias voltage, which is around 3.08 times more than that of pristine TiO2 photoanodes (Jph = 1.20 mA cm−2). This investigation highlights the advantages of the MIL-100(Fe) network in improving the solar PEC-WO performance of TiO2 photoanodes.
Synthesis of n-Butene via Dimethyl Ether-to-Olefin Reaction over P-Loaded Ferrierite Zeolites
Authors: Hanaoka Toshiaki , Aoyagi Masaru , Edashige Yusuke
Published: December 2024

In the dimethyl ether (DME)-to-olefin (DTO) reaction over 20 types of P-loaded ferrierite zeolites with different P loading amounts, the synthesis of n-butenes such as 1-butene, trans-2-butene, and cis-2-butene was investigated to maximize the n-butene yield by optimizing the P loading amount. The zeolites were characterized using X-ray diffractometry (XRD), N2 adsorption-desorption isotherms, and NH3 temperature-programmed desorption (NH3-TPD). Micropore and external surface areas, total pore and micropore volumes, and weak and strong acids affected the DTO reaction’s characteristics. The P-loaded ferrierite zeolite with a P loading of 0.3 wt.% calcined at 500 °C exhibited an n-butene yield of 35.7 C-mol%, which exceeds the highest yield reported to date (31.2 C-mol%). Multiple regression analysis using the obtained data showed that the strong acid/weak acid ratio and total pore volume had a high correlation with the n-butene yield, with a contribution rate of 64.3%. Based on the multiple regression analysis results, the DTO reaction mechanism was discussed based on the proposed reaction model involving the dual-cycle mechanism.
Advances in Valorization of Biomass-Derived Glycolic Acid Toward Polyglycolic Acid Production
Authors: Liu Congzhi , Yan Guochun , Gao Jie , Guo Haixin , Hou Qidong
Published: December 2024

Glycolic acid (GA) is a versatile two-carbon organic chemical with multiple applications in industry and daily life. Currently, GA production depends heavily on the coal chemical industry. In this context, the sustainable production of GA from renewable resources has garnered significant attention. With the design and development of various catalytic systems, the yield of GA from biomass-based feedstocks has been improved observably. Poly(glycolic acid) (PGA) is an aliphatic polyester that exhibits a unique crystalline structure, excellent gas barrier properties, high mechanical strength, superior biocompatibility, and biodegradability. It has a wide range of applications in various fields, such as medical devices, oil extraction, bottle materials, film materials, and textile materials. This article comprehensively elaborates on the methods for the biomass-based synthesis of glycolic acid, the precursor of polyglycolic acid (PGA), as well as the preparation process of PGA. It fills the research gap regarding the sources of biomass raw materials for polyglycolic acid. Additionally, it delves into various modification strategies for PGA and provides an overview of its current applications in multiple fields, including biomedicine, food packaging materials, oil and gas resource development, and agricultural cultivation. The purpose of this article is to provide comprehensive reference information on the synthesis techniques, modification methods, and practical applications of PGA. Furthermore, it offers guidance for research on biodegradable plastics and the biomass-based synthesis of glycolic acid.