bluuebunny/crossref_metadata_2025 huggingface dataset:
Case report: Robotically visualized and biopsy-confirmed peritoneal carcinomatosis as initial identification of metastatic prostate adenocarcinoma in a patient with a history of prostatic urethral lift
Authors: Khan Qateeb , Myers Bryn , Bowar Breann , Khan Maryam , Mullaney Henry , Gainey Jordan , Schneider Robert , Dahmoush Laila , Nepple Kenneth G. , Byrne James D.
Published: January 2024

Background

Peritoneal carcinomatosis is a particularly rare presentation of prostate cancer. Here we report a rare clinical case of surgically identified peritoneal carcinomatosis at the time of a planned robotic prostatectomy in a patient with a history of prostatic urethral lift procedure.

Case presentation

A 72-year-old man, with a history of urinary retention managed with tamsulosin, presented to his local urologist. Prostatic urethral lift procedures were performed for symptom management. After a definitive uptrend in his prostate-specific antigen (PSA) values, a biopsy was obtained, which demonstrated prostate adenocarcinoma. On presurgical multidisciplinary review, it was presumed that he had very high-risk localized prostate cancer. However, upon initiation of robotically assisted laparoscopic radical prostatectomy (RALP), he was noted to have numerous punctate white plaques on the peritoneum; biopsy of these lesions confirmed metastatic disease—for which the patient was starting on triple therapy per the PEACE-1 trial. The PSA level responded appropriately, decreasing from 16.8 to 0.08. Genetic testing was performed and returned negative for any clinically significant mutations.

Conclusion

Our patient, diagnosed with peritoneal carcinomatosis during a planned RALP, highlights the importance of vigilant laparoscopic exam prior to this prostatectomy. Multidisciplinary discussion is crucial for individualized and optimal treatment planning.
TERT promoter methylation is associated with high expression of TERT and poor prognosis in papillary thyroid cancer
Authors: Li Shiyong , Xue Junyu , Jiang Ke , Chen Yulu , Zhu Lefan , Liu Rengyun
Published: January 2024

The telomerase reverse transcriptase (TERT) is overexpressed and associated with poor prognosis in papillary thyroid cancer (PTC), the most common subtype of thyroid cancer. The overexpression of TERT in PTC was partially attributed to transcriptional activation by two hotspot mutations in the core promoter region of this gene. As one of the major epigenetic mechanisms of gene expression regulation, DNA methylation has been proved to regulate several tumor-related genes in PTC. However, the association of TERT promoter DNA methylation with TERT expression and PTC progression is still unclear. By treating PTC cell lines with demethylating agent decitabine, we found that the TERT promoter methylation and the genes’ expression were remarkably decreased. Consistently, PTC patients with TERT hypermethylation had significantly higher TERT expression than patients with TERT hypomethylation. Moreover, TERT hypermethylated patients showed significant higher rates of poor clinical outcomes than patients with TERT hypomethylation. Results from the cox regression analysis showed that the hazard ratios (HRs) of TERT hypermethylation for overall survival, disease-specific survival, disease-free interval (DFI) and progression-free interval (PFI) were 4.81 (95% CI, 1.61-14.41), 8.28 (95% CI, 2.14-32.13), 3.56 (95% CI, 1.24-10.17) and 3.32 (95% CI, 1.64-6.71), respectively. The HRs for DFI and PFI remained significant after adjustment for clinical risk factors. These data suggest that promoter DNA methylation upregulates TERT expression and associates with poor clinical outcomes of PTC, thus holds the potential to be a valuable prognostic marker for PTC risk stratification.
Pain during the first year after scoliosis surgery in adolescents, an exploratory, prospective cohort study
Authors: de Leeuw Thomas G. , Boerlage Anneke A. , van West Hanneke M. , Renkens Jeroen J. M. , van Rosmalen Joost , Staals Lonneke M. E. , Weber Frank , Tibboel Dick , de Wildt Saskia N.
Published: January 2024

Objective

Approximately 50% of adolescents who have undergone scoliosis surgery still experience severe pain one year postoperatively. We explored the postoperative pain trajectory and the potential value of preoperative Thermal Quantitative Sensory Testing (T-QST) as predictor of chronic postsurgical pain after scoliosis surgery.

Design

Single-center prospective cohort study in adolescents undergoing scoliosis surgery.

Outcomes

Prevalence of chronic postsurgical pain (CPSP) one year after scoliosis surgery and postsurgical pain course during this year. The need for rescue medication and the relationship between pre-operative T-QST, acute pain and CPSP.

Results

Thirty-nine patients (mean age 13.9 years; SD 1.9 years) completed the study. One year postoperatively, ten patients (26%) self-reported pain [numeric rating scale (NRS) score ≥ 4]) when moving and two (5%) when in rest. Four of these patients (10.3%) experienced neuropathic pain. The pre-operative cold pain threshold was lower (p = 0.002) in patients with CPSP at 12 months. Preoperative cold and heat pain thresholds were correlated with the number of moderate or severe pain reports (NRS ≥ 4) in the first week postoperatively (r -.426; p = 0.009 and r.392; p = 0.016, respectively).

Conclusions

One year after scoliosis surgery, a significant part of patients (26%) still reported pain, some with neuropathic characteristics. Better diagnosis and treatment is needed; our study suggests that T-QST could be further explored to better understand and treat children with this negative outcome.
The potential mechanism of Aidi injection against neuroblastoma—an investigation based on network pharmacology analysis
Authors: Dai Shuyang , Gu Yaoyao , Zhan Yong , Zhang Jie , Xie Lulu , Li Yi , Lu Yifei , Yang Ran , Zhou Enqing , Chen Deqian , Liu Songbin , Zheng Shan , Shi Zhaopeng , Dong Kuiran , Dong Rui
Published: January 2024

Background: Aidi injection, a classic traditional Chinese medicine (TCM) formula, has been used on a broader scale in treating a variety of cancers. In this study, we aimed to explore the potential anti-tumor effects of Aidi injection in the treatment of neuroblastoma (NB) using network pharmacology (NP).

Methods: To elucidate the anti-NB mechanism of Aidi injection, an NP-based approach and molecular docking validation were employed. The compounds and target genes were collected from the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database and Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine (BATMAN-TCM) database. The protein–protein interaction network was constructed using the STRING database. clusterProfiler (R package) was utilized to annotate the bioinformatics of hub target genes. The gene survival analysis was performed on R2, a web-based genomic analysis application. iGEMDOCK was used for molecular docking validation, and GROMACS was utilized to validate molecular docking results. Furthermore, we investigated the anticancer effects of gomisin B and ginsenoside Rh2 on human NB cells using a cell viability assay. The Western blot assay was used to validate the protein levels of target genes in gomisin B- and ginsenoside Rh2-treated NB cells.

Results: A total of 2 critical compounds with 16 hub target genes were identified for treating NB. All 16 hub genes could potentially influence the survival of NB patients. The top three genes (EGFR, ESR1, and MAPK1) were considered the central hub genes from the drug–compound–hub target gene–pathway network. The endocrine resistance and estrogen signaling pathways were identified as the therapeutic pathways using the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Gomisin B and ginsenoside Rh2 showed a good binding ability to the target protein in molecular docking. The results of cell experiments showed the anti-NB effect of gomisin B and ginsenoside Rh2. In addition, the administration of gomisin B over-regulated the expression of ESR1 protein in MYCN-amplified NB cells.

Conclusion: In the present study, we investigated the potential pharmacological mechanisms of Aidi against NB and revealed the anti-NB effect of gomisin B, providing clinical evidence of Aidi in treating NB and establishing baselines for further research.
Towards a better detection of patients at-risk of linezolid toxicity in clinical practice: a prospective study in three Belgian hospital centers
Authors: Thirot Hélène , Fage David , Leonhardt Antonia , Clevenbergh Philippe , Besse-Hammer Tatiana , Yombi Jean Cyr , Cornu Olivier , Briquet Caroline , Hites Maya , Jacobs Frédérique , Wijnant Gert-Jan , Wicha Sebastian G. , Cotton Frédéric , Tulkens Paul M. , Spinewine Anne , Van Bambeke Françoise
Published: January 2024

Introduction: Linezolid is a last-resort antibiotic for infections caused by multidrug-resistant microorganisms. It is widely used for off-label indications and for longer than recommended treatment durations, exposing patients at higher risk of adverse drug reactions (ADRs), notably thrombocytopenia. This study aimed to investigate ADR incidence and risk factors, identify thrombocytopenia-related trough levels based on treatment duration, and evaluate the performance of predictive scores for ADR development.

Methods: Adult in- and outpatients undergoing linezolid therapy were enrolled in three hospitals and ADRs and linezolid trough levels prospectively monitored over time. A population pharmacokinetic (pop-PK model) was used to estimate trough levels for blood samples collected at varying times.

Results: A multivariate analysis based on 63 treatments identified treatment duration ≥10 days and trough levels >8 mg/L as independent risk factors of developing thrombocytopenia, with high trough values correlated with impaired renal function. Five patients treated for >28 days did not develop thrombocytopenia but maintained trough values in the target range (<8 mg/L). The Buzelé predictive score, which combines an age-adjusted Charlson comorbidity index with treatment duration, demonstrated 77% specificity and 67% sensitivity to predict the risk of ADR.

Conclusion: Our work supports the necessity of establishing guidelines for dose adjustment in patients with renal insufficiency and the systematic use of TDM in patients at-risk in order to keep trough values ≤8 mg/L. The Buzelé predictive score (if ≥7) may help to detect these at-risk patients, and pop-PK models can estimate trough levels based on plasma samples collected at varying times, reducing the logistical burden of TDM in clinical practice.
Protective role of curcumin in disease progression from non-alcoholic fatty liver disease to hepatocellular carcinoma: a meta-analysis
Authors: Li Yubing , Deng Xinyu , Tan Xiyue , Li Qianrong , Yu Zhi , Wu Wenbin , Ma Xiao , Zeng Jinhao , Wang Xiaoyin
Published: January 2024

Background: Pathological progression from non-alcoholic fatty liver disease (NAFLD) to liver fibrosis (LF) to hepatocellular carcinoma (HCC) is a common dynamic state in many patients. Curcumin, a dietary supplement derived from the turmeric family, is expected to specifically inhibit the development of this progression. However, there is a lack of convincing evidence.

Methods: The studies published until June 2023 were searched in PubMed, Web of Science, Embase, and the Cochrane Library databases. The SYstematic Review Center for Laboratory animal Experimentation (SYRCLE) approach was used to evaluate the certainty of evidence. StataSE (version 15.1) and Origin 2021 software programs were used to analyze the critical indicators.

Results: Fifty-two studies involving 792 animals were included, and three disease models were reported. Curcumin demonstrates a significant improvement in key indicators across the stages of NAFLD, liver fibrosis, and HCC. We conducted a detailed analysis of common inflammatory markers IL-1β, IL-6, and TNF-α, which traverse the entire disease process. The research results reveal that curcumin effectively hinders disease progression at each stage by suppressing inflammation. Curcumin exerted hepatoprotective effects in the dose range from 100 to 400 mg/kg and treatment duration from 4 to 10 weeks. The mechanistic analysis reveals that curcumin primarily exerts its hepatoprotective effects by modulating multiple signaling pathways, including TLR4/NF-κB, Keap1/Nrf2, Bax/Bcl-2/Caspase 3, and TGF-β/Smad3.

Conclusion: In summary, curcumin has shown promising therapeutic effects during the overall progression of NAFLD–LF–HCC. It inhibited the pathological progression by synergistic mechanisms related to multiple pathways, including anti-inflammatory, antioxidant, and apoptosis regulation.
A hemodynamic analysis of energy loss in abdominal aortic aneurysm using three-dimension idealized model
Authors: Wang Lulu , Jiang Xudong , Zhang Kejia , Chen Kai , Wu Peng , Li Xiaoqiang
Published: January 2024

Objective: The aim of this study is to perform specific hemodynamic simulations of idealized abdominal aortic aneurysm (AAA) models with different diameters, curvatures and eccentricities and evaluate the risk of thrombosis and aneurysm rupture.

Methods: Nine idealized AAA models with different diameters (3 cm or 5 cm), curvatures (0° or 30°) and eccentricities (centered on or tangent to the aorta), as well as a normal model, were constructed using commercial software (Solidworks; Dassault Systemes S.A, Suresnes, France). Hemodynamic simulations were conducted with the same time-varying volumetric flow rate extracted from the literature and 3-element Windkessel model (3 EWM) boundary conditions were applied at the aortic outlet. Several hemodynamic parameters such as time-averaged wall shear stress (TAWSS), oscillatory shear index (OSI), relative residence time (RRT), endothelial cell activation potential (ECAP) and energy loss (EL) were obtained to evaluate the risk of thrombosis and aneurysm rupture under different conditions.

Results: Simulation results showed that the proportion of low TAWSS region and high OSI region increases with the rising of aneurysm diameter, whereas decreases in the curvature and eccentric models of the corresponding diameters, with the 5 cm normal model having the largest low TAWSS region (68.5%) and high OSI region (40%). Similar to the results of TAWSS and OSI, the high ECAP and high RRT areas were largest in the 5 cm normal model, with the highest wall-averaged value (RRT: 5.18 s, ECAP: 4.36 Pa−1). Differently, the increase of aneurysm diameter, curvature, and eccentricity all lead to the increase of mean flow EL and turbulent EL, such that the highest mean flow EL (0.82 W · 10−3) and turbulent EL (1.72 W · 10−3) were observed in the eccentric 5 cm model with the bending angle of 30°.

Conclusion: Collectively, increases in aneurysm diameter, curvature, and eccentricity all raise mean flow EL and turbulent flow EL, which may aggravate the damage and disturbance of flow in aneurysm. In addition, it can be inferred by conventional parameters (TAWSS, OSI, RRT and ECAP) that the increase of aneurysm diameter may raise the risk of thrombosis, whereas the curvature and eccentricity appeared to have a protective effect against thrombosis.
Advancing tree genomics to future proof next generation orchard production
Authors: Kerr Stephanie C. , Shehnaz Saiyara , Paudel Lucky , Manivannan Mekaladevi S. , Shaw Lindsay M. , Johnson Amanda , Velasquez Jose Teodoro J. , Tanurdžić Miloš , Cazzonelli Christopher I. , Varkonyi-Gasic Erika , Prentis Peter J.
Published: January 2024

The challenges facing tree orchard production in the coming years will be largely driven by changes in the climate affecting the sustainability of farming practices in specific geographical regions. Identifying key traits that enable tree crops to modify their growth to varying environmental conditions and taking advantage of new crop improvement opportunities and technologies will ensure the tree crop industry remains viable and profitable into the future. In this review article we 1) outline climate and sustainability challenges relevant to horticultural tree crop industries, 2) describe key tree crop traits targeted for improvement in agroecosystem productivity and resilience to environmental change, and 3) discuss existing and emerging genomic technologies that provide opportunities for industries to future proof the next generation of orchards.
Reading profiles in secondary school: concurrent language and cognitive abilities, and retrospective and prospective reading skills
Authors: Waldmann Christian , Levlin Maria
Published: January 2024

Introduction

We examined the concurrent language and cognitive abilities in a group of Swedish students with different reading profiles in secondary school, and the retrospective (primary school) and prospective (upper-secondary school) reading skills of each reading profile.

Methods

Seventy-nine students participated in data collections in primary (grade 2: age 8), secondary (grade 8: age 14) and upper-secondary school (year 2: age 17). Independent variables included measures of word recognition, and vocabulary and text comprehension in secondary school. Dependent variables included measures of phonemic awareness, verbal fluency, listening comprehension, spelling, verbal working memory and nonverbal reasoning skills in secondary school, and word recognition and reading comprehension in primary and upper-secondary school.

Results

When exploring the concurrent language and cognitive abilities of the reading profiles in secondary school, spelling emerged as a weakness and listening comprehension as a strength for students with poor decoding. Students with poor comprehension experienced weaknesses in spelling, and non-verbal reasoning. Students with both poor decoding and comprehension displayed a multi-deficit profile in language and cognition. As regards the retrospective and prospective reading skills, the relative ranking of the reading profiles was rather consistent in both primary and upper-secondary school.

Discussion

The findings suggest that limitations in phonological awareness may not be a prominent feature of secondary school students with poor decoding in more transparent orthographies. From an educational perspective, spoken sources may support learning among students with poor decoding, whereas students with poor comprehension or combined difficulties in decoding and comprehension need support when learning from both spoken and written sources.
The associations among gratitude, job crafting, teacher-student relationships, and teacher psychological well-being
Authors: Zheng Xue , Huang Hezi , Yu Quanda
Published: January 2024

Introduction

This study explores the complex dynamics among gratitude, job crafting, teacher psychological well-being, and teacher-student relationships within the context of Chinese English as a Foreign Language (EFL) teachers.

Methods

A sample of 456 Chinese EFL teachers participated in this study. Valid scales were administered to collect data on gratitude, job crafting, teacher psychological well-being, and teacher-student relationships. Structural Equation Modeling (SEM) was employed to investigate these relationships.

Results

The findings reveal significant connections between gratitude, job crafting, teacher psychological well-being, and teacher-student relationships. SEM analysis demonstrates that gratitude and job crafting have direct effects on teacher psychological well-being. Furthermore, teacher-student relationships were identified as a mediator in these relationships.

Discussion

This study underscores the importance of gratitude and job crafting in enhancing the psychological well-being of EFL teachers. It highlights the mediating role of positive teacher-student relationships in the associations between gratitude, job crafting, and teacher psychological well-being. These results have implications for the development of interventions and practices aimed at promoting gratitude, job crafting, and positive teacher-student relationships in the EFL teaching context.
Promoting mental health in esports
Authors: Kegelaers Jolan , Trotter Michael G. , Watson Matthew , Pedraza-Ramirez Ismael , Bonilla Iván , Wylleman Paul , Mairesse Olivier , Van Heel Martijn
Published: January 2024

There is growing recognition of the demands and health challenges experienced by esports athletes. The purpose of this perspective paper is to draw specific attention to the mental health of competitive gamers and spur on both future research and applied initiatives focussing on this important but under-addressed topic. We will briefly discuss the prevalence of mental health concerns, domain-specific stressors, and the need for comprehensive mental health support systems tailored to the esports context. It is our hope that, with this perspective paper, we can help set a new research agenda addressing mental health in esports.
Prevalence of depression and anxiety among elderly primary care patients in Palestine
Authors: Maraqa Beesan Nader , Nazzal Zaher , Hamshari Suha , Alutt Barlant , Rishmawi Ekram , Qawasmeh Abdallah
Published: January 2024

Background and objectives

Depression and anxiety are common mental health disorders among the elderly worldwide. In this study, we estimated the prevalence of depression and anxiety and related risk factor among elderly attending Primary Health care (PHC) centers in Palestine.

Methods

A cross-sectional study was conducted from February to July 2021 with a sample size of 380 participants aged ≥60 attending PHC centers in West Bank, using an interviewer-administered questionnaire. We used the Geriatric Depression Scale-15 and the Geriatric anxiety Scale to screen for depression and anxiety, respectively. Logistic regression models were used to identify predictors of depression and anxiety.

Results

The prevalence of depression and anxiety was 41.1 and 39.2%, respectively. Elderly people living in rural areas (aOR = 2.63; 95% CI: 1.72–4.20), uneducated (aOR = 2.92; 95% CI: 1.41–6.13), and without monthly income (aOR = 3.42; 95% CI: 1.52–7.61) were more likely to have depression. On the other hand, anxiety was independently associated with living in rural areas (aOR = 1.93, 95% CI: 1.23–3.04) and having non-communicable diseases (aOR = 2.01; 95% CI: 1.13–3.49).

Conclusion

Depression and anxiety are common in Palestine, a developing country with a lack of elderly related services. This should be emphasized at the national and regional levels where geriatric health care services are scarce. Such information is required by policymakers and external funding agencies in order to develop future agendas.
The impact of regional origin on the incidence of gestational diabetes mellitus in a multiethnic European cohort
Authors: Kotzaeridi Grammata , Monod Cécile , Linder Tina , Eppel Daniel , Seidel Vera , Feichtinger Michael , Mosimann Beatrice , Filippi Valeria , Wegener Silke , Henrich Wolfgang , Tura Andrea , Göbl Christian S.
Published: January 2024

Introduction

Women with migration background present specific challenges related to risk stratification and care of gestational diabetes mellitus (GDM). Therefore, this study aims to investigate the role of ethnic origin on the risk of developing GDM in a multiethnic European cohort.

Methods

Pregnant women were included at a median gestational age of 12.9 weeks and assigned to the geographical regions of origin: Caucasian Europe (n = 731), Middle East and North Africa countries (MENA, n = 195), Asia (n = 127) and Sub-Saharan Africa (SSA, n = 48). At the time of recruitment maternal characteristics, glucometabolic parameters and dietary habits were assessed. An oral glucose tolerance test was performed in mid-gestation for GDM diagnosis.

Results

Mothers with Caucasian ancestry were older and had higher blood pressure and an adverse lipoprotein profile as compared to non-Caucasian mothers, whereas non-Caucasian women (especially those from MENA countries) had a higher BMI and were more insulin resistant. Moreover, we found distinct dietary habits. Non-Caucasian mothers, especially those from MENA and Asian countries, had increased incidence of GDM as compared to the Caucasian population (OR 1.87, 95%CI 1.40 to 2.52, p < 0.001). Early gestational fasting glucose and insulin sensitivity were consistent risk factors across different ethnic populations, however, pregestational BMI was of particular importance in Asian mothers.

Discussion

Prevalence of GDM was higher among women from MENA and Asian countries, who already showed adverse glucometabolic profiles at early gestation. Fasting glucose and early gestational insulin resistance (as well as higher BMI in women from Asia) were identified as important risk factors in Caucasian and non-Caucasian patients.
What contributes to the long-term implementation of an evidence-based early childhood intervention: a qualitative study from Germany
Authors: Schepan Marie Lisanne , Jungmann Tanja , Kliem Sören , Siegert Carolin , Sandner Malte , Brand Tilman
Published: January 2024

Background

Rigorous research trials have demonstrated that early childhood interventions can reach socially disadvantaged families and can have a lasting impact on the healthy development of their children. However, little is known about the internal and contextual factors that contribute to the long-term implementation of such interventions. In this study, we investigated the development of the home visiting program Pro Kind. The program was adapted from the evidence-based US-American Nurse-Family Partnership program and was implemented in Germany in 2006. Using an exploratory approach, we examined factors contributing to the long-term implementation of this program.

Methods

Qualitative interviews with program implementers (midwives, social workers, program managers) of the Pro Kind program and key stakeholders in two cities in Germany were conducted. Interview guides were developed to assess participants' perceptions and experiences on how the program had developed over time internally and in the interaction with its environment. Data were collected between March and September 2021. Drawing on the Consolidated Framework for Implementation Research (CFIR), data was coded according to the principles of thematic analysis.

Results

A total of 25 individuals (11 program implementers, 14 key stakeholders) were interviewed. The identified factors related to three out of five domains of the CFIR model in our analysis. First, regarding the intervention characteristics, the evidence of effectiveness and the relative advantage of the implementation of the program compared to similar interventions were viewed as contributors to long-term implementation. However, the program's adaptability was discussed as a constraining factor for reaching the target group. Second, concerning the inner setting, stakeholders and program implementers perceived the implementation climate, the leadership engagement and the program's size as relevant factors for networking strategies and program visibility. Third, as part of the outer setting, the degree of networking with external stakeholders was highlighted of great importance for the program.

Conclusions

We identified several factors of particular importance for the long-term implementation and sustainability of an early childhood intervention at the practice level, particularly in the local context in Germany. These findings should inform the design of impactful, scalable, and sustainable early childhood interventions targeting disadvantaged families.
A triple whammy: how urban heat, housing unaffordability and disadvantage affect urban spatial resilience
Authors: Herath Shanaka , Cilliers Elizelle Juanee , Mussi Eveline
Published: January 2024

Climate change generates multiple negative impacts for cities, such as the urban heat island effect. Social stresses, including social disadvantage and housing unaffordability, compound the poor living conditions of urban residents and make our cities less resilient. This paper considers a climate event (urban heat) and social stresses (housing affordability and urban disadvantage) to explore urban resilience in Sydney. We draw on a framework for building urban climate resilience, incorporating facets of urban resilience, social stresses that amplify crises, and solutions that strengthen individuals and communities with coping abilities to withstand climate events. The study diverges from aggregate city-level analyses that hide small-area differences in climate impacts and vulnerability. The findings reveal the spatiality of these natural and social impacts, identifying 11 critical areas in Sydney impacted by the highest levels of urban heat and urban disadvantage, and two critical areas impacted by the highest levels of urban heat and housing unaffordability. We highlight the importance of context-based approaches and place-based policies to address climate risks and social vulnerabilities on the path toward creating more resilient cities.
Green Labs: How Scientists Can Help Fight Climate Change
Authors: Sesen Julie , Ragland Stephanie , Lagomarsino Valentina , Busatto Sara
Published: January 2024

The healthcare industry creates 4.4% of the world’s carbon emissions. To put this into perspective, if the healthcare industry were a country, it would be the fifth-largest carbon emitter on the planet. The goal of the healthcare industry is to create new, life-saving treatments for patients, but it also produces a lot of waste that cannot be recycled, such as plastic and chemicals. Additionally, this industry uses a lot of non-renewable energy. The Green Lab Project is a worldwide, 10-year-old program attempting to reduce the carbon emissions caused by the activities of scientists within the healthcare industry. The Green Lab project arrived at Boston Children’s Hospital (BCH) in 2020, at the request of a group of postdocs, graduate students, and employees. The BCH Green Labs group is working to sponsor environment-protecting projects and educate BCH scientists to adopt more responsible, informed, and “greener” work practices. This article will describe ways that BCH scientists are trying to reduce lab waste and energy consumption, including sharing equipment and materials to limit waste production, cleaning freezers regularly, and using outlet timers to avoid wasting energy.
Understanding the drivers of rural household scaling up of integrated crop-livestock-forestry systems. A systematic review and bibliometric analysis
Authors: Kotobiodjo Mahugnon Néhémie , Egah Janvier , Dato Stéphenson Marcel , Baco Mohamed Nasser
Published: January 2024

This literature review focuses on the drivers of Integrated Crop-Livestock-Forestry Systems (ICLFS) adoption by small farmers. It has enabled us to identify current trends and analyze the various drivers of adoption and knowledge gaps. According to the PRISMA literature review protocol, 1,352 scientific publications have been selected and analyzed after a Scopus database search. Data analysis was carried out in two steps. A systematic review was performed with the metadata of scientific publications using the Biblioshiny package of R 4.3.1 software. Then, the 42 most relevant publications were used for a brief narrative synthesis. The results showed that between 2003 and 2023, publications were made in 587 different sources. 73% of publications were scientific articles. 91% of publications were written by an average of 05 co-authors. The effectiveness of Integrated Crop-Livestock-Forestry Systems (ICLFS) practices, the production of organic matter and the effects on farmers’ livelihoods are the research topics considered. Five categories of factors were identified to facilitate the adoption of ICLFS by small farmers: (i) farmer profile, (ii) farm characteristics, (iii) economic factors, (iv) institutional factors and (v) biophysical factors. Policy orientations are the most decisive of all the factors identified, followed by the establishment of extension systems and social networks between farmers. This paper makes three main recommendations. Firstly, it recommends the implementation of collaborative research frameworks between West African researchers and those from East Africa, Asia and South America, who have more experience in this area. In addition, this study suggests that future research on the adoption of ICLFS should take into account herd mobility issues in the adoption process. Finally, it suggests that ICLFS should be taken into account in development policies and implemented through action research projects, mostly in West Africa.
Development and Experimentation of Intra-Row Weeding Device for Organic Rice
Authors: Jiao Jinkang , Hu Lian , Chen Gaolong , Chen Chaowen , Zang Ying
Published: January 2024

Weeds in paddy fields can seriously reduce rice yield. An intra-row weeding device with double-layer elastic rods was designed, considering the differences in mechanical properties between rice and weeds, which can press weeds into the soil and avoid damaging rice. The elastic force of the elastic rods can be adjusted by changing the position of the regulating mechanism to adapt to different weeding conditions. A measurement experiment was conducted to determine the variation rule of elastic force. The quadratic orthogonal rotation combination discrete element simulation experiment, which used weeding depth and weeding speed as experimental factors, and the amount of soil disturbance and the force of the inner and outer elastic rod in the horizontal and vertical directions as experimental indicators, was conducted to study the interaction between the weeding device and the soil. The optimal weeding parameters were obtained: the weeding depth was 15 mm, the weeding speed was 0.9 m/s. The field experiment, which used the various parameters of the weeding device as experimental factors and the weeding rate and damaging seedling rate as experimental indicators, was conducted to determine the weeding effect. The experimental results showed that the optimal position of the regulating mechanism was 270 mm, with a weeding rate of 80.65% and a damaging seedling rate of 3.36%. The weeding rate can be increased by at least 11.18% by adjusting the regulating mechanism to a suitable position under the same weeding conditions. This study can provide a reference for research on weeding machinery for organic rice.
The Content of Antioxidant Compounds and VOCs in Sorghum Grain Grown in Central and Eastern Europe
Authors: Przybylska-Balcerek Anna , Frankowski Jakub , Sieracka Dominika , Sázavská Tereza , Wacławek Stanisław , Raczak Barbara Klaudia , Szwajkowska-Michałek Lidia , Buśko Maciej , Graczyk Małgorzata , Niedziela Grażyna , Stuper-Szablewska Kinga
Published: January 2024

Sorghum is a plant belonging to the Poaceae family. It is drought-resistant and has low soil requirements. In the face of climate change, it is increasingly cultivated in Europe. Poland is a country with great agricultural potential; it is thus important to develop effective and economic methods of agricultural production, which is confirmed by the introduction of sorghum into cultivation. The aim of this study was to characterize the composition of bioactive compounds (i.e., phenolic acids, flavonoids, carotenoids, and phytosterols) and VOCs in sorghum grain of two varieties, i.e., white ‘Sweet Caroline’ and red ‘Sweet Susana’ grown in the temperate climate (Pętkowo, Poland (52°12′40″ N 17°15′31″ E)). The following tests were carried out: analysis of phenolic acids, flavonoids, carotenoids, phytosterols, antioxidant activity (ABTS), free phenolic acids (FPAs); elemental analysis; and water, fat and starch content analysis. Based on the conducted research, it was concluded that Poland has appropriate conditions for growing sorghum, as the content of bioactive (antioxidant) compounds was at a similar level to those grown in tropical and subtropical climates. Of the nine phenolic acids and seven flavonoids determined, the highest concentrations in both sorghum grain varieties were found for ferulic, p-coumaric and protocatechuic acids. The content of ferulic acid was three times higher in Sweet Caroline grains than in Sweet Susana grains. Differences in the content of these compounds may be the result of genetic differences between the Sweet Susana and Sweet Caroline varieties.
Synergistic Effects of Bicarbonate and Selenium on Cadmium Transport in Karst-Adaptable Plants Based on Plant Electrical Signals
Authors: Xia Antong , Wu Yanyou , Qin Zhanghui , Zhu Yunfen , Li Lin , Xiao Juyue , Aboueldahab Mohamed , Wan Haiying , Ming Jiajia , Xiang Jiqian
Published: January 2024

High cadmium (Cd) concentrations associated with geochemical anomalies are prevalent in carbonate-rich karstic areas, posing serious ecological risks, while the karstic soils are rich in bicarbonate (HCO3−). It is known that Selenium (Se) is a mineral element that effectively mitigates the Cd transport in plant species. However, the synergistic effects of HCO3− and Se on Cd translocation in plant species have not yet been indicated. In this study, based on plant electrophysiological measurements, we chose Orychophragmus violaceus (Ov) as experimental material and determined the growth potential, water metabolism, and nutrient translocation capacity under HCO3−-Se6+-Cd2+ treatment to identify the synergistic effects of bicarbonate and selenium on cadmium transport in karst-adaptable plants. We found 5 mM HCO3− and 0.46 mM Se6+ synergistically alleviated the Cd2+ stress on Ov, promoting growth, intracellular water metabolism, nutrient translocation efficiency, and total Se accumulation, and inhibiting the uptake of Cd in Ov. Nevertheless, 15 mM HCO3− and 0.46 mM Se6+ synergistically augmented Cd transport on Ov, resulting in growth reduction, intracellular water metabolism, nutrient translocation efficiency, and total Se concentration. Hence, it not only provides a frontier approach for the real-time monitoring of intracellular water and nutrient utilization capacity in plant species, but is also even more a theoretical idea that reveals the synergistic effects of bicarbonate and selenium on cadmium transport of plants to precisely indicate selenium supplementation to ameliorate cadmium pollution and construct a sustainable karst ecosystem in the future.
Identification and Functional Analysis of Cynoglossus semilaevis Z-Linked E3 Ubiquitin Ligase rnf34
Authors: Li Lu , Li Xihong , Chen Yadong , Yang Yingming , Wang Na , Xu Wenteng
Published: January 2024

The high proportion of males in C. semilaevis hinders their industrial development. The genetic ZW individual can become a pseudomale by sex reversal. And the pseudomale can produce Z-sperm (with epigenetic information to cause sex reversal) while W-sperm is absent, which leads to an even higher male proportion in offspring. Recently, with the development of transcriptomic technologies, research on spermatogenesis in C. semilaevis has been focused on the ubiquitination pathway. In this study, we analyzed the function of the ubiquitin ligase rnf34 gene on the Z chromosome. A qPCR experiment showed that its expression level in the gonad was the highest among different tissues. In the ovary, the expression gradually increased with development from 40 days post-hatching (dph) to 1.5 years post-hatching (yph). In the testis, rnf34 showed increased expression from 40 dph to 6 months post-hatching (mpf) and stabilized up until 1.5 ypf. In situ hybridization showed that the mRNA of rnf34 was mainly distributed in the germ cells of the testis and the ovary. In vivo siRNA-mediated knockdown of the rnf34 gene in male fish affected the expression of a series of genes related to sex differentiation and spermatogenesis. These results provide genetic data on the molecular mechanisms of gonadal development and spermatogenesis in C. semilaevis.
Protective Effects of Niacin on Rumen Epithelial Cell Barrier Integrity in Heat-Stressed Beef Cattle
Authors: Zou Bicheng , Long Fan , Xue Fuguang , Chen Chuanbin , Zhang Xian , Qu Mingren , Xu Lanjiao
Published: January 2024

The present study investigates the theoretical basis for maintaining normal physiological functions in heat-stressed beef cattle by exploring the effects of niacin supplementation on the permeability of the rumen epithelial cell barrier. Herein, 12 Jinjiang bulls with an average weight of approximately 400 ± 20.0 kg were randomly divided into three groups, thermoneutral (TN), heat-stressed (HS), and heat-stressed niacin-supplemented (HN) groups, with 4 bulls in each group. The experiment spanned 70 days, and the plasma concentrations of D-lactic acid, diamine oxidase (DAO), lipopolysaccharides (LPSs), and inflammatory cytokines were analyzed. Additionally, we assessed the gene expression of tight junction proteins to understand the effect of niacin supplementation on heat-stressed beef cattle. Our results revealed that heat stress significantly increased the D-lactic acid and LPS levels in beef cattle plasma on days 30 and 45 of the experiment (p < 0.05). Moreover, it led to a significant rise in DAO levels on day 30 (p < 0.05). Niacin supplementation significantly reduced the LPS levels on day 30 (p < 0.05). Heat stress significantly elevated the plasma concentrations of inflammatory cytokines interleukin-1β (IL-1β), IL-2, IL-6, and tumor necrosis factor-α (TNF-α) (p < 0.05), while reducing the IL-4 concentration (p < 0.05). However, niacin supplementation effectively mitigated the concentrations of these inflammatory factors by reducing IL-1β, IL-2, IL-6, and TNF-α concentrations and increasing IL-4 concentrations. The mRNA expressions of tight junction proteins zonula occluden-1 (ZO-1), claudin-1, claudin-4, and claudin-7 were significantly downregulated (p < 0.05) in the HS group compared to those in the TN group, and those of ZO-1 and occludin were significantly upregulated (p < 0.05) in the HN group compared to those in the HS group. Notably, no significant differences were observed in ruminal papillae length and width among the studied groups (p > 0.05). Our findings indicate that heat stress adversely impacted the tight junction structure of the rumen epithelium, leading to a significant reduction in the expression of tight junction protein mRNA. Consequently, heat stress impaired the rumen mucosal barrier function, resulting in increased intestinal permeability. The mechanism underlying this effect may be associated with the decreased expression of tight junction protein genes in the rumen epithelial cells. However, niacin supplementation mitigated the detrimental effects of heat stress on intestinal permeability in beef cattle and increased the expression of tight junction protein genes in the rumen epithelium, thereby effectively protecting the rumen barrier in heat-stressed beef cattle. These results highlight the potential of nicotinic acid as a protective agent against the negative impacts of heat stress on intestinal integrity in beef cattle.
Does Acidification Affect Urinary Creatinine in Dairy Cattle?
Authors: Danese Tommaso , Sabetti Maria Chiara , Mezzasalma Nicolò , Simoni Marica , Quintavalla Cecilia , Righi Federico
Published: January 2024

Nitrogen content in urine plays a crucial role in assessing the environmental impact of dairy farming. Urine acidifications avoid urine nitrogen volatilization, but potentially lead to a degradation of creatinine, the most dependable marker for quantifying total urine excretion volume, affecting its measurement. This study aimed to assess how acidifying urine samples affects the concentration and detection of creatinine in dairy cattle. In this trial, individual urine samples from 20 Holstein lactating dairy cows were divided into three subsamples, allocated to 1 of 3 groups consisting of 20 samples each. Samples were immediately treated as follows: acidification with H2SO4 (1 mL of acid in 30 mL of sample) to achieve a pH < 2 (Group 1)); addition of an equal volume of distilled water (1 mL of distilled water in 30 mL of sample) to investigate dilution effects (Group 2); or storage without any acid or water treatment (Group 3). An analysis of creatinine levels was carried out using the Jaffe method. The Friedman test was employed to compare urine groups across treatments, and the Bland–Altman test was used to assess the agreement between measurements in Group 1 and Group 3. Urinary creatinine values were statistically different (p < 0.001) between Group 1 (median 48.5 mg/dL; range 36.9–83 mg/dL), Group 2 (median 47.5 mg/dL; range 36.5–80.7 mg/dL), and Group 3 (median 48.9 mg/dL, range 37.2–84). Bland–Altman analysis demonstrates agreement between Group 3 and Group 1. The measurement of urinary creatinine using the Jaffe method is affected by sample acidification, but the use of creatinine as a marker for total urine output could remain a viable tool when urine samples are acidified.
Punicalagin Protects Ram Sperm from Oxidative Stress by Enhancing Antioxidant Capacity and Mitochondrial Potential during Liquid Storage at 4 °C
Authors: Zhang Liuming , Wang Xuyang , Sohail Tariq , Jiang Caiyu , Sun Yuxuan , Wang Jian , Sun Xiaomei , Li Yongjun
Published: January 2024

The aim of this study was to investigate the effect of punicalagin, an antioxidant, on ram sperm quality. Semen samples were collected and pooled from five rams, then diluted using a Tris-based diluent containing various concentrations (0, 5, 15, 30 and 45 μM) of punicalagin. Sperm motility, plasma membrane integrity, acrosome integrity, total antioxidant capacity (TAC), reactive oxygen species (ROS), malondialdehyde (MDA), mitochondrial membrane potential (MMP), superoxide dismutase (SOD) and catalase (CAT) were measured and analyzed during liquid storage at 4 °C. The results showed that the Tris-based solution containing punicalagin improved sperm motility, plasma membrane integrity, acrosome integrity, TAC, SOD, CAT and MMP, and decreased ROS content and MDA content. At the same time, the semen sample diluted with the Tris-based solution supplemented with 30 μM punicalagin achieved the best effect. The sperm total motility, progressive motility, plasma membrane integrity, acrosome integrity, TAC, SOD, CAT and MMP of the group supplemented with 30 μM punicalagin were significantly (p < 0.05) higher than those of the other groups on the 5th day during the liquid storage at 4 °C. Meanwhile, the ROS content and MDA content were significantly (p < 0.05) lower than those in the other groups. In conclusion, the optimal concentration of punicalagin in the Hu ram semen diluent was determined to be 30 μM. The results indicated that a diluent supplemented with punicalagin could enhance the quality of ram sperm preserved at 4 °C by increasing antioxidant capacity, mitochondrial potential and reducing oxidative stress.