bluuebunny/crossref_metadata_2025 huggingface dataset:
Zooshikella harenae sp. nov., Isolated from Pacific Oyster Crassostrea gigas, and Establishment of Zooshikella ganghwensis subsp. marina subsp. nov. and Zooshikella ganghwensis subsp. ganghwensis subsp. nov.
Authors: Pira Hani , Risdian Chandra , Kämpfer Peter , Müsken Mathias , Schupp Peter J. , Wink Joachim
Published: December 2021

Here, we describe the polyphasic taxonomy of a novel isolated strain WH53T from the genus Zooshikella isolated from the sand sediment located between the lumen of the Crassostrea gigas From Germany. Phylogenetic analysis determined that the strain WH53T had a high similarity to Zooshikella ganghwensis JC2044T (99.57%) and Zooshikella marina LMG 28823T (99.36%). Strain WH53T contained ubiquinone-9 (Q-9) as the predominant menaquinone, and the major fatty acids were C16:0, C16:1ω7c, and C18:1ω7c. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, amino phospholipids, and unidentified phospholipids were identified as their polar lipid composition. The DNA G+C content and genome size of strain WH53T were 40.08 mol% and 5,914,969 bp, respectively. Digital DNA–DNA hybridisation (dDDH) for strain WH53T against Z. ganghwensis JC2044T and Z. marina LMG 28823T showed low relatedness values of 26.3% and 26.1%, respectively. The extract of strain WH53T exhibited antimicrobial property. Strain WH53T represents a novel species in the genus Zooshikella. We propose the name of Zooshikella harenae sp. nov., with the type strain WH53T (= DSM 111628T = NCCB 100808T). Furthermore, the dDDH, average nucleotide identity (ANI), percentage of conserved proteins (POCP), and amino acid identity (AAI) value between Z. marina LGM 28823T and Z. ganghwensis DSM 15267T were 79.9%, 97.84%, 76.08%, and 87.01%, respectively, suggesting that both of them should be reclassified as Z. ganghwensis subsp. marina subsp. nov. and Z. ganghwensis subsp. ganghwensis DSM 15267 subsp. nov.
Prognostic Accuracy of the qSOFA Score for In-Hospital Mortality in Elderly Patients with Obstructive Acute Pyelonephritis: A Multi-Institutional Study
Authors: Ishikawa Yudai , Fukushima Hiroshi , Tanaka Hajime , Yoshida Soichiro , Yokoyama Minato , Matsuoka Yoh , Sakai Yasuyuki , Otsuka Yukihiro , Takazawa Ryoji , Yano Masataka , Tsukamoto Tetsuro , Okuno Tetsuo , Noro Akira , Nagahama Katsushi , Kamata Shigeyoshi , Fujii Yasuhisa
Published: December 2021

Prognostic accuracy of the quick sequential organ failure assessment (qSOFA) score for mortality may be limited in elderly patients. Using our multi-institutional database, we classified obstructive acute pyelonephritis (OAPN) patients into young and elderly groups, and evaluated predictive performance of the qSOFA score for in-hospital mortality. qSOFA score ≥ 2 was an independent predictor for in-hospital mortality, as was higher age, and Charlson comorbidity index (CCI) ≥ 2. In young patients, the area under the curve (AUC) of the qSOFA score for in-hospital mortality was 0.85, whereas it was 0.61 in elderly patients. The sensitivity and specificity of qSOFA score ≥ 2 for in-hospital mortality was 80% and 80% in young patients, and 50% and 68% in elderly patients, respectively. For elderly patients, we developed the CCI-incorporated qSOFA score, which showed higher prognostic accuracy compared with the qSOFA score (AUC, 0.66 vs. 0.61, p < 0.001). Therefore, the prognostic accuracy of the qSOFA score for in-hospital mortality was high in young OAPN patients, but modest in elderly patients. Although it can work as a screening tool to determine therapeutic management in young patients, for elderly patients, the presence of comorbidities should be considered at the initial assessment.
Explainable Convolutional Neural Network to Investigate Age-Related Changes in Multi-Order Functional Connectivity
Authors: Dong Sunghee , Jin Yan , Bak SuJin , Yoon Bumchul , Jeong Jichai
Published: December 2021

Functional connectivity (FC) is a potential candidate that can increase the performance of brain-computer interfaces (BCIs) in the elderly because of its compensatory role in neural circuits. However, it is difficult to decode FC by the current machine learning techniques because of a lack of physiological understanding. To investigate the suitability of FC in BCIs for the elderly, we propose the decoding of lower- and higher-order FC using a convolutional neural network (CNN) in six cognitive-motor tasks. The layer-wise relevance propagation (LRP) method describes how age-related changes in FCs impact BCI applications for the elderly compared to younger adults. A total of 17 young adults 24.5±2.7 years and 12 older 72.5±3.2 years adults were recruited to perform tasks related to hand-force control with or without mental calculation. The CNN yielded a six-class classification accuracy of 75.3% in the elderly, exceeding the 70.7% accuracy for the younger adults. In the elderly, the proposed method increased the classification accuracy by 88.3% compared to the filter-bank common spatial pattern. The LRP results revealed that both lower- and higher-order FCs were dominantly overactivated in the prefrontal lobe, depending on the task type. These findings suggest a promising application of multi-order FC with deep learning on BCI systems for the elderly.
RPPUF: An Ultra-Lightweight Reconfigurable Pico-Physically Unclonable Function for Resource-Constrained IoT Devices
Authors: Huang Zhao , Li Liang , Chen Yin , Li Zeyu , Wang Quan , Jiang Xiaohong
Published: December 2021

With the advancement of the Internet of Things (IoTs) technology, security issues have received an increasing amount of attention. Since IoT devices are typically resource-limited, conventional security solutions, such as classical cryptography, are no longer applicable. A physically unclonable function (PUF) is a hardware-based, low-cost alternative solution to provide security for IoT devices. It utilizes the inherent nature of hardware to generate a random and unpredictable fingerprint to uniquely identify an IoT device. However, despite existing PUFs having exhibited a good performance, they are not suitable for effective application on resource-constrained IoT devices due to the limited number of challenge-response pairs (CRPs) generated per unit area and the large hardware resources overhead. To solve these problems, this article presents an ultra-lightweight reconfigurable PUF solution, which is named RPPUF. Our method is built on pico-PUF (PPUF). By incorporating configurable logics, one single RPPUF can be instantiated into multiple samples through configurable information K. We implement and verify our design on the Xilinx Spartan-6 field programmable gate array (FPGA) microboards. The experimental results demonstrate that, compared to previous work, our method increases the uniqueness, reliability and uniformity by up to 4.13%, 16.98% and 10.5%, respectively, while dramatically reducing the hardware resource overhead by 98.16% when a 128-bit PUF response is generated. Moreover, the bit per cost (BPC) metric of our proposed RPPUF increased by up to 28.5 and 53.37 times than that of PPUF and the improved butterfly PUF, respectively. This confirms that the proposed RPPUF is ultra-lightweight with a good performance, making it more appropriate and efficient to apply in FPGA-based IoT devices with constrained resources.
A Carrier-Based Discontinuous PWM Strategy of NPC Three-Level Inverter for Common-Mode Voltage and Switching Loss Reduction
Authors: Zhang Guozheng , Su Yingjie , Zhou Zhanqing , Geng Qiang
Published: December 2021

For the conventional carrier-based pulse width modulation (CBPWM) strategies of neutral point clamped (NPC) three-level inverters, the higher common-mode voltage (CMV) is a major drawback. However, with CMV suppression strategies, the switching loss is relatively high. In order to solve the above issue, a carrier-based discontinuous PWM (DPWM) strategy for NPC three-level inverter is proposed in this paper. Firstly, the reference voltage is modified by the twice injection of zero-sequence voltage. Switching states of the three-phase are clamped alternatively to reduce both the CMV and the switching loss. Secondly, the carriers are also modified by the phase opposite disposition of the upper and lower carriers. The extra switching at the border of two adjacent regions in the space vector diagram is reduced. Meanwhile, a neutral-point voltage (NPV) control method is also presented. The duty cycle of the switching state that affects the NPV is adjusted to obtain the balance control of the NPV. Still, the switching sequence in each carrier period remains the same. Finally, the feasibility and effectiveness of the proposed DPWM strategy are tested on a rapid control prototype platform based on RT-Lab.
Quantifying Ecological Performance of Giant Panda Conservation: Evidence from Sichuan Province
Authors: Song Zhenjiang , Li Yi
Published: December 2021

The giant panda (Ailuropoda melanoleuca) is a symbolic and flagship species in the field of endangered wildlife conservation. We studied the changing and driving factors of landscape patterns in Sichuan giant panda habitats through image interpretation and ecological niche evaluation models. According to land-use and cover-change analysis, we also studied the structural changes in habitat over the past two decades and used empirical analysis to evaluate the relative ecological niche widths and overlap of giant panda distribution areas in 1995 and 2015. It is found the area of non-forested land decreased significantly from 1995 to 2015. It is interesting that the high-quality land-use types tended to decrease but low/middle-quality land-use types tended to increase over the past 20 years. Giant panda conservation projects in China have promoted changes in conservation thought and management, as well as the innovation of technical means over the studied period. The goals of Chinese giant panda conservation projects are not only to facilitate giant panda reproduction but also to alleviate the contradiction between conservation and development and promote the coexistence of humans and giant pandas.
Spectroscopic Stability Studies of Pressure Sensitive Labels Facestock Made from Recycled Post-Consumer Waste and Agro-Industrial By-Products
Authors: Vukoje Marina , Itrić Ivanda Katarina , Kulčar Rahela , Marošević Dolovski Ana
Published: December 2021

To support circular economy and sustainability, the use of synthetic polymers should be minimized due to their excessive accumulation in the environment and low biodegradation rate which leads to a global waste problem. Thus, the quota of natural resources should be increased, as well as the use of agriculture and industrial waste or by-products as raw materials in industrial processes. Agro-industrial wastes were found to have high cellulose fiber potential which makes them an excellent resource for paper production. This study investigates the influence of artificial aging test on the stability of commercially available fiber based self-adhesive pressure sensitive labels (PSL), in which the facestock is made of 15% agro-industrial byproducts, 40% post-consumer recycled paper and 45% virgin wood pulp and compared to ones made of biobased polyethylene. The results showed that optical brighteners are present in the composition of fiber based PSL. Moreover, the fluorescence and UV-Visible spectroscopy results indicated that by the action of UV irradiation and temperature. The oxidation of cellulose was not found by FTIR spectroscopy, indicating its high stability.
Transcriptomic Analysis Reveals Regulatory Networks for Osmotic Water Stress and Rewatering Response in the Leaves of Ginkgo biloba
Authors: Yu Wanwen , Cai Jinfeng , Liu Huimin , Lu Zhiguo , Hu Jingjing , Lu Yan
Published: December 2021

To elucidate the transcriptomic regulation mechanisms that underlie the response of Ginkgo biloba to dehydration and rehydration, we used ginkgo saplings exposed to osmotically driven water stress and subsequent rewatering. When compared with a control group, 137, 1453, 1148, and 679 genes were differentially expressed in ginkgo leaves responding to 2, 6, 12, and 24 h of water deficit, and 796 and 1530 genes were differentially expressed responding to 24 and 48 h of rewatering. Upregulated genes participated in the biosynthesis of abscisic acid, eliminating reactive oxygen species (ROS), and biosynthesis of flavonoids and bilobalide, and downregulated genes were involved in water transport and cell wall enlargement in water stress-treated ginkgo leaves. Under rehydration conditions, the genes associated with water transport and cell wall enlargement were upregulated, and the genes that participated in eliminating ROS and the biosynthesis of flavonoids and bilobalide were downregulated in the leaves of G. biloba. Furthermore, the weighted gene coexpression networks were established and correlated with distinct water stress and rewatering time-point samples. Hub genes that act as key players in the networks were identified. Overall, these results indicate that the gene coexpression networks play essential roles in the transcriptional reconfiguration of ginkgo leaves in response to water stress and rewatering.
Selenomethionine-Dominated Selenium-Enriched Peanut Protein Ameliorates Alcohol-Induced Liver Disease in Mice by Suppressing Oxidative Stress
Authors: Gao Lin , Yuan Jiawei , Cheng Yuhuan , Chen Mengling , Zhang Genhua , Wu Jihong
Published: December 2021

Numerous natural compounds are considered as potential therapeutic agents against alcohol-induced liver disease (ALD). Research shows that selenium (Se) has a variety of bioactivities, including liver protecting ability. The present study based on in vitro cell culture models and in vivo mouse models was aimed at examining the contribution of selenomethionine (SeMet)-dominated Se-enriched peanut protein (SePP) to liver protection. SeMet and especially SePP reversed cell viability and cell death, inhibited ethanol induced CYP2E1 activation, decreased reactive oxygen species level, and restored GSH level. Hence, SeMet-dominated SePP alleviates alcohol-induced AML-12 cytotoxicity by suppressing oxidative stress. The p38-dependent mechanism was found to be responsible for SePP-induced Nrf-2 activation. Furthermore, supplementation with SePP and SeMet regulated lipid metabolism and reduced oxidative stress, minimizing liver damage in mice. Selenomethionine-dominated SePP possesses potential therapeutic properties and can be used to treat ALD through the suppression of oxidative stress.
Fingerprint Approaches Coupled with Chemometrics to Discriminate Geographic Origin of Imported Salmon in China’s Consumer Market
Authors: Fu Xianshu , Hong Xuezhen , Liao Jinyan , Ji Qingge , Li Chaofeng , Zhang Mingzhou , Ye Zihong , Yu Xiaoping
Published: December 2021

Of the salmon sold in China’s consumer market, 92% was labelled as Norwegian salmon, but was in fact was mainly imported from Chile. The aim of this study was to establish an effective method for discriminating the geographic origin of imported salmon using two fingerprint approaches, Near-infrared (NIR) spectroscopy and mineral element fingerprint (MEF). In total, 80 salmon (40 from Norway and 40 from Chile) were tested, and data generated by NIR and MEF were analysed via various chemometrics. Four spectral preprocessing methods, including vector normalization (VN), Savitzky Golay (SG) smoothing, first derivative (FD) and second derivative (SD), were employed on the raw NIR data, and a partial least squares (PLS) model based on the FD + SG9 pretreatment could successfully differentiate Norwegian salmons from Chilean salmons, with a R2 value of 98.5%. Analysis of variance (ANOVA) and multiple comparative analysis were employed on the contents of 16 mineral elements including Pb, Fe, Cu, Zn, Al, Sr, Ni, As, Cr, V, Se, Mn, K, Ca, Na and Mg. The results showed that Fe, Zn, Al, Ni, As, Cr, V, Se, Ca and Na could be used as characteristic elements to discriminate the geographical origin of the imported salmon, and the discrimination rate of the linear discriminant analysis (LDA) model, trained on the above 10 elements, could reach up to 98.8%. The results demonstrate that both NIR and MEF could be effective tools for the rapid discrimination of geographic origin of imported salmon in China’s consumer market.
Evaluation of Phytochemical Contents and In Vitro Antioxidant, Anti-Inflammatory, and Anticancer Activities of Black Rice Leaf (Oryza sativa L.) Extract and Its Fractions
Authors: Thepthanee Chorpaka , Liu Chan-Chiung , Yu Hsu-Sheng , Huang Ho-Shin , Yen Chia-Hung , Li Yen-Hsien , Lee Maw-Rong , Liaw Ean-Tun
Published: December 2021

Black rice leaves (Oryza sativa L.) are a major part of rice straw left in open fields after rice harvest as agricultural waste. In this study, crude ethanolic extract (CEE) and various solvent fractions (hexane (Hex), ethyl acetate (EtOAc), n-butanol (n-BuOH), and aqueous fractions) of black rice leaves were investigated for their bioactive compound contents as well as antioxidant, anti-inflammatory, and anticancer activities. The results demonstrated that among all the fractions, the n-BuOH fraction presented the greatest contents of total phenolics and flavonoids, while anthocyanins were found to be abundant in the n-BuOH and aqueous fractions, which also exhibited powerful antioxidant abilities according to DPPH and ABTS radical-scavenging assays and a reducing power assay. Regarding anti-inflammatory activity, CEE and EtOAc reduced the production of NO and cytokine secretion (PGE2, IL-6, and IL-1β) but displayed less effect on tumor necrosis factor α (TNF-α) release in lipopolysaccharide (LPS)-induced RAW 264.7 cells. They also significantly decreased iNOS and COX-2 protein expression. Additionally, the phenolics-rich ethyl acetate fraction showed the greatest activity against HepG2 liver carcinoma cells, inhibited cell growth, increased the Sub-G1 population, and induced apoptosis via mitochondrion-dependent mechanisms. In conclusion, black rice leaves, a byproduct of rice, exhibited strong antioxidant, anti-inflammatory, and anticancer capacities and might be useful for application in functional foods and the pharmaceutical industry.
Proteomic Changes in Sarcoplasmic and Myofibrillar Proteins Associated with Color Stability of Ovine Muscle during Post-Mortem Storage
Authors: Gao Xiaoguang , Zhao Dandan , Wang Lin , Cui Yue , Wang Shijie , Lv Meng , Zang Fangbo , Dai Ruitong
Published: December 2021

The objective of this study was to investigate the proteomic characteristics for the sarcoplasmic and myofibrillar proteomes of M. longissimus lumborum (LL) and M. psoasmajor (PM) from Small-tailed Han Sheep. During post-mortem storage periods (1, 3, and 5 days), proteome analysis was applied to elucidate sarcoplasmic and myofibrillar protein changes in skeletal muscles with different color stability. Proteomic results revealed that the identified differentially abundant proteins were glycolytic enzymes, energy metabolism enzymes, chaperone proteins, and structural proteins. Through Pearson’s correlation analysis, a few of those identified proteins (Pyruvate kinase, Adenylate kinase isoenzyme 1, Creatine kinase M-type, and Carbonic anhydrase 3) were closely correlated to representative meat color parameters. Besides, bioinformatics analysis of differentially abundant proteins revealed that the proteins mainly participated in glycolysis and energy metabolism pathways. Some of these proteins may have the potential probability to be predictors of meat discoloration during post-mortem storage. Within the insight of proteomics, these results accumulated some basic theoretical understanding of the molecular mechanisms of meat discoloration.
Dietary Acrylamide Intake Alters Gut Microbiota in Mice and Increases Its Susceptibility to Salmonella Typhimurium Infection
Authors: Wang Zhen , Liu Hongxu , Liu Jiaxiu , Ren Xiaomeng , Song Guoku , Xia Xiaodong , Qin Ningbo
Published: December 2021

Acrylamide (AA) has been extensively examined for its potential toxicological effects on humans and animals, but its impacts on gut microbiota and effects on hosts’ susceptibility to enteric infection remain elusive. The present study was designed to evaluate the effect of AA on gut microbiota of mice and susceptibility of mice to S. Typhimurium infection. After four weeks’ intervention, mice fed with AA exhibited significantly decreased body weight. Meanwhile, 16S rRNA gene sequencing showed reduced relative abundance of Firmicutes and increased abundance of Bacteroidetes in AA-treated mice prior to infection. In addition, we observed high relative abundance of Burkholderiales and Erysipelotrichales, more specifically the genus Sutterella and Allobaculum, respectively, in AA-treated mice before infection. Subsequently, the mice were orally infected with S. Typhimurium. The histological changes, systemic dissemination of S. Typhimurium, and inflammatory responses were examined. Compared to mice fed with normal diet, mice fed AA exhibited higher level of bacterial counts in liver, spleen, and ileum, which was consistent with exacerbated tissue damage determined by histological analyses. In addition, higher expression of pro-inflammaroty cytokines, p-IκBα, and p-P65 and lower mRNA expressions of mucin2, occludin, zo-1, claudin-1, and E-cadherin were detected in AA-treated mice. These findings provide novel insights into the potential health impact of AA consumption and the detailed mechanism for its effect on S. Typhimurium infection merit further exploration.
High-Pressure Homogenization and Biocontrol Agent as Innovative Approaches Increase Shelf Life and Functionality of Carrot Juice
Authors: Gottardi Davide , Siroli Lorenzo , Braschi Giacomo , Rossi Samantha , Ferioli Federico , Vannini Lucia , Patrignani Francesca , Lanciotti Rosalba
Published: December 2021

Recently, application of high-pressure homogenization (HPH) treatments has been widely studied to improve shelf life and rheological and functional properties of vegetable and fruit juices. Another approach that has drawn the attention of researchers is the use of biocontrol cultures. Nevertheless, no data on their possible combined effect on fruit juices shelf life and functionality have been published yet. In this work, the microbial, organoleptic, and technological stability of extremely perishable carrot juice and its functionality were monitored for 12 and 7 days (stored at 4 and 10 °C, respectively) upon HPH treatment alone or in combination with a fermentation step using the biocontrol agent L. lactis LBG2. HPH treatment at 150 MPa for three passes followed by fermentation with L. lactis LBG2 extended the microbiological shelf life of the products of at least three and seven days when stored at 10 °C and 4 °C, respectively, compared to untreated or only HPH-treated samples. Moreover, the combined treatments determined a higher stability of pH and color values, and a better retention of β-carotene and lutein throughout the shelf-life period when compared to unfermented samples. Eventually, use of combined HPH and LBG2 resulted in the production of compounds having positive sensory impact on carrot juice.
A Study on the Clustering of Extra Virgin Olive Oils Extracted from Cultivars Growing in Four Ionian Islands (Greece) by Multivariate Analysis of Their Phenolic Profile, Antioxidant Activity and Genetic Markers
Authors: Kalaboki Iliana , Koulougliotis Dionysios , Kleisiari Dimitra , Melliou Eleni , Magiatis Prokopios , Kampioti Adamantia , Eriotou Effimia , Destouni Aspasia
Published: December 2021

Background: The phenolic fraction of extra virgin olive oil (EVOO) has disease preventive and health-promoting properties which are supported by numerous studies. As such, EVOO is defined as a functional food. The aim of the present study was to characterize the phenolic profile of olive oil from cultivars farmed in the Ionian Islands (Zakynthos, Kefalonia, Lefkada, and Kerkyra) and to investigate the association of phenols to antioxidant activity, which is central to its functionality. Furthermore, the study investigates whether multivariate analyses on the concentration of individual biophenolic compounds and genetic population diversity could classify the olive oil samples based on their geographic origin. Methods: Phenols were determined in 103 samples from different Ionian Island tree populations by 1H nuclear magnetic resonance (NMR), and sample antioxidant activity was measured by their capacity to reduce the free radical 2,2-diphenyl-1-picrylhydrazyl) (DPPH). Genetic diversity was measured by estimating Nei’s population genetic distance using 15 reproducible bands from random amplified polymorphic DNA (RAPD) genotyping. Results: Principal component analysis (PCA) of the secoiridoid concentrations clustered samples according to cultivar. Clustering based on genetic distances is not concordant with phenolic clustering. A cultivar effect was also demonstrated in the association between the concentration of individual phenols with DPPH reducing activity. Conclusions: Taken together, the study shows that the olive oil phenolic content defines “cultivar-specific phenolic profiles” and that environmental factors other than agronomic conditions contribute more to phenotype variance than genetics.
Chufa Drink: Potential in Developing a New Plant-Based Fermented Dessert
Authors: Madsen Sanne Kjærulf , Thulesen Elisabeth Thule , Mohammadifar Mohammad Amin , Bang-Berthelsen Claus Heiner
Published: December 2021

Plant-based foods with desirable texture and nutritional value have attracted considerable interest from consumers. In order to meet the growing demand for more sustainable and health-focused products, new sources for plant-based products are needed. In this study, we aimed to develop an innovative plant-based dessert based on the underutilized crop chufa tubers (Cyperus esculentus). The chufa extract was fermented with plant-adapted lactic acid bacteria and formulated with the purpose of imitating the Danish summer dessert “cold butter-milk soup”. The effect of various bacterial fermentations and formulations on steady and oscillatory rheology, stability, dry matter, pH, and sugar profile of the product were studied and compared to a commercial cold buttermilk soup sample. A strain of Leuconostoc mesenteroides was found to create the most similar taste to a commercial sample. By adding lemon juice, sucrose, xanthan gum, and vanilla to the fermented chufa drink, the drink was found to mimic the pH, texture, acid profile, and stability of a commercial dairy-based sample, while containing a lower concentration of carbohydrates.
Development of Nano Soy Milk through Sensory Attributes and Consumer Acceptability
Authors: Ju Seyoung , Song Sooji , Lee Jeongnam , Hwang Sungwon , Lee Yoonmi , Kwon Yongseok , Lee Yuyoung
Published: December 2021

Nanotechnology is currently applied in food processing and packaging in the food industry. Nano encapsulation techniques could improve sensory perception and nutrient absorption. The purpose of this study was to identify the sensory characteristics and consumer acceptability of three types of commercial and two types of laboratory-developed soy milk. A total of 20 sensory attributes of the five different soy milk samples, including appearance, smell (odor), taste, flavor, and mouthfeel (texture), were developed. The soy milk samples were evaluated by 100 consumers based on their overall acceptance, appearance, color, smell (odor), taste, flavor, mouthfeel (texture), goso flavor (nuttiness), sweetness, repeated use, and recommendation. One-way analysis of variance (ANOVA), principal component analysis (PCA), and partial least square regression (PLSR) were used to perform the statistical analyses. The SM_D sample generally showed the highest scores for overall liking, flavor, taste, mouthfeel, sweetness, repeated consumption, and recommendation among all the consumer samples tested. Consumers preferred sweet, goso (nuttiness), roasted soybean, and cooked soybean (nuttiness) attributes but not grayness, raw soybean flavor, or mouthfeel. Sweetness was closely related to goso (nuttiness) odor and roasted soybean odor and flavor based on partial least square regression (PLSR) analysis. Determination of the sensory attributes and consumer acceptance of soymilk provides insight into consumer needs and desires along with basic data to facilitate the expansion of the consumer market.
An In Vitro Protocol to Study the Modulatory Effects of a Food or Biocompound on Human Gut Microbiome and Metabolome
Authors: Rosés Carles , Nieto Juan Antonio , Viadel Blanca , Gallego Elisa , Romo-Hualde Ana , Streitenberger Sergio , Milagro Fermín I. , Barceló Anna
Published: December 2021

The gut microbiota plays a key role in gastrointestinal immune and metabolic functions and is influenced by dietary composition. An in vitro protocol simulating the physiological conditions of the digestive system helps to study the effects of foods/biocompounds on gut microbiome and metabolome. The Dynamic-Colonic Gastrointestinal Digester consists of five interconnected compartments, double jacket vessels that simulate the physiological conditions of the stomach, the small intestine and the three colonic sections, which are the ascending colon, transverse colon and descending colon. Human faeces are required to reproduce the conditions and culture medium of the human colon, allowing the growth of the intestinal microbiota. After a stabilization period of 12 days, a food/biocompound can be introduced to study its modulatory effects during the next 14 days (treatment period). At the end of the stabilization and treatment period, samples taken from the colon compartments are analysed. The 16S rRNA gene analysis reveals the microbiota composition. The untargeted metabolomics analysis gives more than 10,000 features (metabolites/compounds). The present protocol allows in vitro testing of the modulatory effects of foods or biocompounds on gut microbiota composition and metabolic activity.
Chromosome Number, Ploidy Level, and Nuclear DNA Content in 23 Species of Echeveria (Crassulaceae)
Authors: Palomino Guadalupe , Martínez-Ramón Javier , Cepeda-Cornejo Verónica , Ladd-Otero Miriam , Romero Patricia , Reyes-Santiago Jerónimo
Published: December 2021

Echeveria is a polyploid genus with a wide diversity of species and morphologies. The number of species registered for Echeveria is approximately 170; many of them are native to Mexico. This genus is of special interest in cytogenetic research because it has a variety of chromosome numbers and ploidy levels. Additionally, there are no studies concerning nuclear DNA content and the extent of endopolyploidy. This work aims to investigate the cytogenetic characteristics of 23 species of Echeveria collected in 9 states of Mexico, analyzing 2n chromosome numbers, ploidy level, nuclear DNA content, and endopolyploidy levels. Chromosome numbers were obtained from root tips. DNA content was obtained from the leaf parenchyma, which was processed according to the two-step protocol with Otto solutions and propidium iodide as fluorochrome, and then analyzed by flow cytometry. From the 23 species of Echeveria analyzed, 16 species lacked previous reports of 2n chromosome numbers. The 2n chromosome numbers found and analyzed in this research for Echeveria species ranged from 24 to 270. The range of 2C nuclear DNA amounts ranged from 1.26 pg in E. catorce to 7.70 pg in E. roseiflora, while the 1C values were 616 Mbp and 753 Mbp, respectively, for the same species. However, differences in the level of endopolyploidy nuclei were found, corresponding to 4 endocycles (8C, 16C, 32C and 64C) in E. olivacea, E. catorce, E. juarezensis and E. perezcalixii. In contrast, E. longiflora presented 3 endocycles (8C, 16C and 32C) and E. roseiflora presented 2 endocycles (8C and 16C). It has been suggested that polyploidization and diploidization processes, together with the presence of endopolyploidy, allowed Echeveria species to adapt and colonize new adverse environments.
Editorial of Special Issue “Detecting Geospace Perturbations Caused by Earth”
Authors: Balasis Georgios , De Santis Angelo
Published: December 2021

A systematic multiparametric and multiplatform approach to detect and study geo-space perturbations attributed to preparation processes related to natural hazards is fundamental in order to obtain useful insights on a series of complex dynamic phenomena of the Earth system, namely, earthquakes, volcanic and Saharan dust events, as well as geomagnetic disturbances [...]
Positive Correlation of Serum Resistin Level with Peripheral Artery Disease in Patients with Chronic Kidney Disease Stage 3 to 5
Authors: Ng Xin-Ning , Tang Chi-Chong , Wang Chih-Hsien , Tsai Jen-Pi , Hsu Bang-Gee
Published: December 2021

Chronic kidney disease (CKD) is associated with higher risk of cardiovascular disease-related ischemic events, which includes peripheral arterial disease (PAD). PAD is a strong predictor of future cardiovascular events, which can cause significant morbidity and mortality. Resistin has been found to be involved in pathological processes leading to CVD. Therefore, we aim to investigate whether resistin level is correlated with PAD in patients with non-dialysis CKD stage 3 to 5. There were 240 CKD patients enrolled in this study. Ankle-brachial index (ABI) values were measured using the automated oscillometric method. An ABI value < 0.9 defined the low ABI group. Serum levels of human resistin were determined using a commercially available enzyme immunoassay. Thirty CKD patients (12.5%) were included in the low ABI group. Patients in the low ABI group were older and had higher resistin levels as well as higher diabetes mellitus, hypertension and habit of smoking, and lower estimated glomerular filtration rate than patients in the normal ABI group. After the adjustment for factors that were significantly associated with PAD by multivariate logistic regression analysis, age and serum resistin level were independent predictors of PAD. A high serum resistin level is an independent predictor of PAD in non-dialysis CKD stage 3 to 5.
Contemporary Neuroprotection Strategies during Cardiac Surgery: State of the Art Review
Authors: Motshabi-Chakane Palesa , Mogane Palesa , Moutlana Jacob , Leballo-Mothibi Gontse , Dingezweni Sithandiwe , Mpanya Dineo , Tsabedze Nqoba
Published: December 2021

Open-heart surgery is the leading cause of neuronal injury in the perioperative state, with some patients complicating with cerebrovascular accidents and delirium. Neurological fallout places an immense burden on the psychological well-being of the person affected, their family, and the healthcare system. Several randomised control trials (RCTs) have attempted to identify therapeutic and interventional strategies that reduce the morbidity and mortality rate in patients that experience perioperative neurological complications. However, there is still no consensus on the best strategy that yields improved patient outcomes, such that standardised neuroprotection protocols do not exist in a significant number of anaesthesia departments. This review aims to discuss contemporary evidence for preventing and managing risk factors for neuronal injury, mechanisms of injury, and neuroprotection interventions that lead to improved patient outcomes. Furthermore, a summary of existing RCTs and large observational studies are examined to determine which strategies are supported by science and which lack definitive evidence. We have established that the overall evidence for pharmacological neuroprotection is weak. Most neuroprotective strategies are based on animal studies, which cannot be fully extrapolated to the human population, and there is still no consensus on the optimal neuroprotective strategies for patients undergoing cardiac surgery. Large multicenter studies using universal standardised neurological fallout definitions are still required to evaluate the beneficial effects of the existing neuroprotective techniques.
Effect of Integrated Training on Balance and Ankle Reposition Sense in Ballet Dancers
Authors: Lin Chai-Wei , You Yu-Lin , Chen Yi-An , Wu Tzu-Chan , Lin Cheng-Feng
Published: December 2021

Objective: To investigate the effects of a 6-week integrated training program on the ankle joint reposition sense and postural stability in ballet dancers. Methods: Sixteen female ballet dancers participated in the study and underwent a 6-week integrated training program consisting of plyometric, proprioception and core stability exercises along with a home program involving additional ankle muscle strengthening and stretching. The ankle joint reposition tests and the parameters of the center of pressure (COP) while performing grand-plie (deep squatting) and releve en demi-pointe (standing on balls of foot) movements were measured before and after training. Results: After 6 weeks, participants showed significantly smaller absolute ankle joint reposition errors in dorsiflexion (p = 0.031), plantarflexion (p = 0.003) and eversion (p = 0.019) compared to the pre-training measurement. Furthermore, after training, a significantly slower average COP speed at pre-equilibrium during grand-plie movement (p = 0.003) and pre-equilibrium phase of releve en demi-pointe (p = 0.023) were observed. In addition, the maximum COP displacement in the medial-lateral direction was significantly smaller at pre-equilibrium phase during grand-plie (p = 0.044) and releve en demi-pointe movements (p = 0.004) after training. Conclusions: The 6-week integrated training program improved the ankle joint reposition sense and postural control in the medial-lateral direction during grand-plie and releve en demi-pointe movements.
Effects of Ai-Chi Practice on Balance and Left Cerebellar Activation during High Working Memory Load Task in Older People: A Controlled Pilot Trial
Authors: Nissim Michal , Livny Abigail , Barmatz Caroline , Tsarfaty Galia , Berner Yitshal , Sacher Yaron , Bodini Raffaella , Ratzon Navah Z.
Published: December 2021

Background: Normal aging is associated with balance and working memory decline. From a neurobiological standpoint, changes in cerebellar functional plasticity may mediate the decline in balance and working memory for older adults. Mounting evidence suggests that physical activity is beneficial for decreasing aging effects. Previous studies have focused on land-based physical activity and research concerning the aquatic environment is scarce. This study investigated the effectiveness of Ai-Chi on balance abilities and cerebral activation during a high working memory load task among community-dwelling older people. Methods: A total of 19 people aged 65–86 years were allocated to receive Ai-Chi practice (n = 6), structured on-land Ai-Chi practice (n = 7) or guided-imagery of Ai-Chi practice (n = 6) for a bi-weekly, 30-min exercise session for 12 weeks. Balance was measured by the Tinetti balance sub-test and working memory was measured by the N-back test during functional-MRI scan. Results: The Ai-Chi practice group presented a significant change in balance between pre and post intervention (balance t = −4.8, p < 0.01). In the whole-brain analysis, during high working memory load task, the Ai-Chi practice group presented a decrease in left cerebellar activation. Region of interest analyses yielded similar results by which pre-cerebellar activation was higher than post-intervention (t = 2.77, p < 0.05). Conclusions: Ai-Chi is an available, non-invasive intervention method that may serve as a tool to improve cerebellar activation that in turn might improve balance. In addition, our findings may provide new insights into the neuronal mechanisms that underlie both motor and cognitive abilities.
School-Level Factors within Comprehensive School Health Associated with the Trajectory of Moderate-to-Vigorous Physical Activity over Time: A Longitudinal, Multilevel Analysis in a Large Sample of Grade 9 and 10 Students in Canada
Authors: Pirrie Melissa , Carson Valerie , Dubin Joel A. , Leatherdale Scott T.
Published: December 2021

(1) The majority of Canadian youth are insufficiently active, and moderate-to-vigorous physical activity (MVPA) decreases substantially during secondary school. School factors within the comprehensive school health (CSH) framework may help attenuate this decline. This study aimed to examine how youth MVPA changes over a three-year period and evaluate the school characteristics associated with preventing the decline in MVPA over time, guided by the CSH framework. (2) This study uses COMPASS survey data from 78 secondary schools in Ontario and Alberta that participated in Year 2 (2013/14), Year 3 (2014/15), and Year 4 (2015/16), and 17,661 students attending these schools. Multilevel (linear mixed effects) models were used to determine the association between school-level factors and student MVPA (weekly minutes) over time, stratified by gender. (3) Both male and female students had a significant decline in MVPA across the 3 years, with a greater decrease observed among female students. Within the CSH framework, the school’s social environment, partnerships, and policies were associated with student MVPA over time, however the specific school factors and directions of associations varied by gender. (4) School-based interventions (e.g., public health partnerships) may help avoid the decline in MVPA observed in this critical period and support student health.
Associations between Risk Factors in Late Adolescence and Problem Behaviors in Young Adulthood: A Six-Year Follow-Up of Substance Related and Behavioral Addictions in Swedish High School Seniors
Authors: Andersson Claes , Håkansson Anders
Published: December 2021

Introduction: Risk factors of traditional substance use related problems in young adults are more well-known than for behavioral addictions such as gambling and gaming problems. The present study aims to provide knowledge about the longitudinal patters of substance use related and behavioral addictions in early adulthood. Methods: Using self-report surveys, substance-related, psychiatric, and demographic predictors were assessed in Swedish high school seniors and re-assessed six years later along with gambling and gaming problems, n = 800. Associations (Risk Ratios) between risk factors in late adolescence and problem behaviors in young adulthood were analyzed. Results: Tobacco use, illicit drug use, and hazardous drinking in young adulthood were associated with tobacco use, illicit drug use, alcohol use, conduct problems, and impaired impulse control in late adolescence. Gambling problems in young adulthood were only associated with heredity of alcohol problems, while gaming was not associated to any problem behavior in late adolescence. Conclusion: It is concluded that predictors for traditional substance-related addictions differ from predictors for behavioral addictions, and that this difference is more pronounced for gaming problems than for gambling problems.
Epidemiological Trends of Malaria in Five Years and under Children of Nsanje District in Malawi, 2015–2019
Authors: Gondwe Theodore , Yang Yongi , Yosefe Simeon , Kasanga Maisa , Mulula Griffin , Luwemba Mphatso Prince , Jere Annie , Daka Victor , Mudenda Tobela
Published: December 2021

Background: Malaria continues to be a major public health problem in Malawi and the greatest load of mortality and morbidity occurs in children five years and under. However, there is no information yet regarding trends and predictions of malaria incidence in children five years and under at district hospital level, particularly at Nsanje district hospital. Aim: Therefore, this study aimed at investigating the trends of malaria morbidity and mortality in order to design appropriate interventions on the best approach to contain the disease in the near future. Methodology: Trend analysis of malaria morbidity and mortality together with time series analysis using the SARIMA (Seasonal Autoregressive Integrated Moving Average) model was used to predict malaria incidence in Nsanje district. Results: The SARIMA model used malaria cases from 2015 to 2019 and created the best model to forecast the malaria cases in Nsanje from 2020 to 2022. An SARIMA (0, 1, 2) (0,1,1)12 was suitable for forecasting the incidence of malaria for Nsanje. Conclusion: The mortality and morbidity trend showed that malaria cases were growing at a fluctuating rate at Nsanje district hospital. The relative errors between the actual values and predicted values indicated that the predicted values matched the actual values well. Therefore, the model proved that it was adequate to forecast monthly malaria cases and it had a good fit, hence, was appropriate for this study
Risk of COVID-19 Infection in Public Transportation: The Development of a Model
Authors: Park Junsik , Kim Gurjoong
Published: December 2021

South Korea’s social distancing policies on public transportation only involve mandatory wearing of masks and prohibition of food intake, similar to policies on other indoor spaces. This is not because public transportation is safe from coronavirus disease 2019 (COVID-19), but because no suitable policies based on accurate data have been implemented. To relieve fears regarding contracting COVID-19 infection through public transportation, the government should provide accurate information and take appropriate measures to lower the risk of COVID-19. This study aimed to develop a model for determining the risk of COVID-19 infection on public transportation considering exposure time, mask efficiency, ventilation rate, and distance. The risk of COVID-19 infection on public transportation was estimated, and the effectiveness of measures to reduce the risk was assessed. The correlation between the risk of infection and various factors was identified through sensitivity analysis of major factors. The analysis shows that, in addition to the general indoor space social distancing policy, ventilation system installation, passenger number reduction in a vehicle, and seat distribution strategies were effective. Based on these results, the government should provide accurate guidelines and implement appropriate policies.
Policy Implications of the COVID-19 Pandemic on Food Insecurity in Rural America: Evidence from Appalachia
Authors: Cardarelli Kathryn M. , DeWitt Emily , Gillespie Rachel , Graham Rachel H. , Norman-Burgdolf Heather , Mullins Janet T.
Published: December 2021

Rural communities are disproportionally affected by food insecurity, making them vulnerable to the consequences of supply disruptions caused by the COVID-19 pandemic. While access to food was initially diminished due to food supply disruptions, little is known about the mechanisms through which federal emergency assistance programs impacted food access in rural populations. Through a series of five focus groups in spring 2021, we examined the impact of the COVID-19 pandemic on food access in a rural Appalachian community in Kentucky. Data were analyzed using a Grounded Theory Approach. Findings revealed the following four primary themes: food scarcity in grocery stores; expanded federal food assistance; expanded community food resources; and expanded home gardening. Participants provided details regarding the way increased federal assistance, especially expanded benefits within the Supplemental Nutrition Assistance Program, allowed them to purchase greater quantities of nutritious food. This study unveils the specific impacts of the COVID-19 pandemic on one rural population, including the influence of some social determinants of health on food insecurity. Policymakers and stakeholders should recognize the layered protection of multiple federal emergency assistance programs against food insecurity and the potential for long-term population health promotion in rural areas.