bluuebunny/crossref_metadata_2025 huggingface dataset:
Method of Designing a Friction-Based Wedge Anchorage System for High-Strength CFRP Plates
Authors: Zhu Wanxu , Wei Wei , Liu Fengrong , Zeng Rong
Published: October 2021

The cables of high-strength carbon fiber reinforced polymer (CFRP) plates are starting to be applied to large spatial structures. However, their main anchorage systems rely on the adhesive force, which entails risks to their integrity resulting from aging of the binding agent. In this study, a friction-based wedge anchorage system was designed for CFRP plates. The working mechanism of the proposed anchorage system was explored both theoretically and experimentally. The anti-slip mechanism and condition of CFRP plates were formulated so that the equivalent frictional angle of the contact surface between a CFRP plate and wedges must not be smaller than the sum of the dip angle of the wedge external conical surface and the frictional angle between the wedges and barrel. An analysis of the stress distribution in the anchorage zone of the CFRP plate was conducted using the Tsai-Wu failure criterion, which concluded that the compressive stresses should be reduced on the section closer to the load-bearing end of the anchorage system. Furthermore, the anchorage efficiency coefficient was proposed, which depends on stress concentration coefficients, plate thickness, length of anchorage zone, dip angle of wedge external conical surface, and its frictional angle. Then, it was determined that the minimum length of an anchorage zone for the CFRP plates with various specifications should be at least 49 times larger than the CFRP thickness. A finite element analysis and static tensile tests on six specimens were carried out. The experimental results revealed that the anchorage efficiency coefficient of the optimized anchor reached 97.9%.
Dimensional Accuracy and Surface Quality of AZ91D Magnesium Alloy Components after Precision Milling
Authors: Korpysa Jarosław , Kuczmaszewski Józef , Zagórski Ireneusz
Published: October 2021

This study investigates a precision milling process conducted with the use of conventional end mills and a standard CNC (Computer Numerical Control) machine tool. Milling tests were performed on samples of AZ91D magnesium alloy using TiB2- and TiAlN-coated three-edge end mills measuring 16 mm in diameter. The following technological parameters were made variable: cutting speed, feed per tooth and axial depth of cut. The effects of precision milling were evaluated by analysing the scatter of dimension values obtained in successive tool passes. In addition to that, deviations from the assumed nominal depth as well as obtained ranges of dimension varation were analysed. The study also examined surface quality obtained in the precision milling process, based on the basic surface roughness parameters: Ra, Rz and RSm. Results have confirmed that the use of conventional cutting tools and a standard CNC machine tool makes it possible to manufacture components characterized by relatively small scatter of dimension values and high accuracy classes. Additionally, the results have shown that the type of tool coating and variations of individual technological parameters exert impact on the dimensional accuracy and surface quality obtained.
Diptool—A Novel Numerical Tool for Membrane Interactions Analysis, Applying to Antimicrobial Detergents and Drug Delivery Aids
Authors: Rzycki Mateusz , Kraszewski Sebastian , Gładysiewicz-Kudrawiec Marta
Published: October 2021

The widespread problem of resistance development in bacteria has become a critical issue for modern medicine. To limit that phenomenon, many compounds have been extensively studied. Among them were derivatives of available drugs, but also alternative novel detergents such as Gemini surfactants. Over the last decade, they have been massively synthesized and studied to obtain the most effective antimicrobial agents, as well as the most selective aids for nanoparticles drug delivery. Various protocols and distinct bacterial strains used in Minimal Inhibitory Concentration experimental studies prevented performance benchmarking of different surfactant classes over these last years. Motivated by this limitation, we designed a theoretical methodology implemented in custom fast screening software to assess the surfactant activity on model lipid membranes. Experimentally based QSAR (quantitative structure-activity relationship) prediction delivered a set of parameters underlying the Diptool software engine for high-throughput agent-membrane interactions analysis. We validated our software by comparing score energy profiles with Gibbs free energy from the Adaptive Biasing Force approach on octenidine and chlorhexidine, popular antimicrobials. Results from Diptool can reflect the molecule behavior in the lipid membrane and correctly predict free energy of translocation much faster than classic molecular dynamics. This opens a new venue for searching novel classes of detergents with sharp biologic activity.
Effects of Knee Injury Length on Jump Inside Kick Performances of Wushu Player
Authors: Cha Jun-Youl , Lee Ha-Sung , Park Sihwa , Jee Yong-Seok
Published: October 2021

Background and Objectives: When performing the jump inside kick in Wushu, it is important to understand the rotation technique while in mid-air. This is because the score varies according to the mid-air rotation, and when landing after the mid-air rotation, it causes considerable injury to the knee. This study aimed to compare the differences in kinematic and kinetic variables between experienced and less experienced knee injuries in the Wushu players who perform 360°, 540°, and 720° jump inside kicks in self-taolu. Materials and Methods: The participants’ mean (SD) age was 26.12 (2.84) years old. All of them had suffered knee injuries and were all recovering and returning to training. The group was classified into a group with less than 20 months of injury experience (LESS IG, n = 6) and a group with more than 20 months of injury experience (MORE IG, n = 6). For kinematic measurements, jump inside kicks at three rotations were assessed by using high-speed cameras. For kinetic measurements, the contraction time and maximal displacement of tensiomyography were assessed in the vastus lateralis, vastus medialis, rectus femoris, biceps femoris, gastrocnemius lateralis, gastrocnemius medialis, and tibialis anterior. The peak torque, work per repetition, fatigue index, and total work of isokinetic moments were assessed using knee extension/flexion, ankle inversion/eversion, and ankle plantarflexion/dorsiflexion tests. Results: Although there was no difference at the low difficulty level (360°), there were significant differences at the higher difficulty levels (540° and 720°) between the LESS IG and the MORE IG. For distance and time, the LESS IG had a shorter jump distance, but a faster rotation time compared to those in the MORE IG. Due to the characteristics of the jump inside kick’s rotation to the left, the static and dynamic muscle contractility properties were mainly found to be higher in the left lower extremity than in the right lower extremity, and higher in the LESS IG than in the MORE IG. In addition, this study observed that the ankle plantarflexor in the LESS IG was significantly higher than that in the MORE IG. Conclusion: To become a world-class self-taolu athlete while avoiding knee injuries, it is necessary to develop the static and dynamic myofunctions of the lower extremities required for jumping. Moreover, it is considered desirable to train by focusing on the vertical height and the amount of rotation during jumping.
Rosai–Dorfman Disease: Breast Involvement—Case Report and Literature Review
Authors: Iancu George , Gica Nicolae , Mustata Laura Mihaela , Panaitescu Anca Maria , Vasile Danut , Peltecu Gheorghe
Published: October 2021

Background and objectives: Rosai–Dorfman disease (RDD) is a type of histiocytosis that usually appears in young adults or children as bilateral cervical lymphadenopathy, but extranodal involvement in not uncommon. Although the pathogenesis is not entirely elucidated, recent studies showed a possible neoplastic process. Materials and methods: Our manuscript presents a rare case of Rosai–Dorfman disease of the breast, the management of this rare case, and a literature review. There are few cases reported of RDD of the breast (around 90 globally reported cases); the data is poor, and the management not yet standardized for these cases. The case reported here shows the importance of correct breast investigation, breast imaging, and ultrasound-guided biopsy that provided an accurate diagnosis and guided further management. Results: Although RDD of the breast was rarely presented as bilateral disease in other case reports, our case showed bilateral breast disease with the suspicion of breast cancer on imaging. Pathology and immunohistochemistry were of critical importance and showed a specific pattern for histiocytosis. A multidisciplinary approach was taken into consideration for these cases in order to establish the approach. Some patients underwent surgery, but watchful waiting and close follow-up were the preferred approach. Conclusions: RDD of the breast is a rare form of histiocytosis, with fewer than 100 globally published cases. Although the management of this disease is not established yet by guidelines, a follow-up approach should be enough for these patients, and surgery might be overtreatment. Mortality from RDD is very low due to comorbidities. A multidisciplinary team decision is important, and abstinence might significantly benefit these patients.
Pathways to Acceptance in Participants of Advanced Cancer Online Support Groups
Authors: Pereira Christina Francesca , Cheung Kate , Alie Elyse , Wong Jiahui , Esplen Mary Jane , Leung Yvonne W.
Published: October 2021

Background and Objectives: Individuals with cancer, especially advanced cancer, are faced with numerous difficulties associated with the disease, including an earlier death than expected. Those who are able to confront and accept the hardships associated with the disease in a way that aligns with their beliefs benefit from more positive psychological outcomes compared to those who are aware of their diagnosis but are unable to accept it. To date, there is limited research exploring factors contributing to illness and death acceptance in the context of advanced cancer in group therapy settings. Materials and Methods: The current study used a Directed Content Analysis approach on transcripts of online advanced cancer support groups to investigate if and how Yalom’s existential factors played a role in the emergence of acceptance. Results: The online support group platform, combined with the help of facilitators, offered supportive environments for individuals seeking help with cancer-related distress by helping patients move towards acceptance. Some participants had already begun the process of accepting their diagnosis before joining the group, others developed acceptance during the group process, while a few continued to be distressed. Our analysis revealed the emergence of four themes related to illness acceptance: (1) Facilitator-Initiated Discussion, including sub-themes of Mindfulness, Relaxation and Imagery, Changing Ways of Thinking, and Spirituality; (2) Personal attitudes, including sub-themes of Optimism and Letting Go of Control; (3) Supportive Environment, including the sub-themes of Providing Support to Others and Receiving Support from Others; and (4) Existential Experience, which included sub-themes of Living with the Diagnosis for an Extended Amount of Time, Legacy and Death Preparations, and Appreciating life. Conclusions: With a paradigm shift to online delivery of psychological services, recognizing factors that contribute to acceptance when dealing with advanced cancer may help inform clinical practices. Future studies should explore patient acceptance longitudinally to inform whether it emerges progressively, which has been suggested by Kübler-Ross.
Effect of Gravity and Various Operating Conditions on Proton Exchange Membrane Water Electrolysis Cell Performance
Authors: Choi Yena , Lee Woojung , Na Youngseung
Published: October 2021

Water electrolysis is an eco-friendly method for the utilization of renewable energy sources which provide intermittent power supply. Proton exchange membrane water electrolysis (PEMWE) has a high efficiency in this regard. However, the two-phase flow of water and oxygen at the anode side causes performance degradation, and various operating conditions affect the performance of PEMWE. In this study, the effects of four control parameters (operating temperature, flow rate, cell orientation, and pattern of the channel) on the performance of PEMWE were investigated. The effects of the operating conditions on its performance were examined using a 25 cm2 single-cell. Evaluation tests were conducted using in situ methods such as polarization curves and electrochemical impedance spectroscopy. The results demonstrated that a high operating temperature and low flow rate reduce the activation and ohmic losses, and thereby enhance the performance of PEMWE. Additionally, the cell orientation affects the performance of PEMWE owing to the variation in the two-phase flow regime. It was observed that the slope of specific sections in the polarization curve rapidly increases at a specific cell voltage.
Characterization of Cryopreserved Canine Amniotic Membrane
Authors: Withavatpongtorn Nathawan , Tuntivanich Nalinee
Published: October 2021

Amniotic membrane is an effective corneal reconstruction material in veterinary surgery. Cryopreserved amniotic membrane is widely used in practice. Properties of cryopreserved canine amniotic membranes are currently not well studied. This study aimed to compare three properties between canine amniotic membranes cryopreserved for 7 days and 30 days, including tensile strength, transparency, and cell viability. After their respective cryopreservation time, stress–strain curves of the cryopreserved membranes’ tensile strength were assessed using a universal testing machine. Both groups produced J-shaped stress–strain curves with statistically comparable parameters, including maximum stress, strain, and Young’s modulus. The percentage of cell viability was observed by trypan blue staining under a light microscope. Membrane transparency was tested with a spectrophotometer. Transparency tests showed high levels of light transmission and low haze, with no statistical difference between groups. Cell viability was statistically lower in the 30-day cryopreserved group. Tensile strength and transparency of cryopreserved CAM were not significantly impeded for up to 30 days. For CAM to be used as an alternative corneal transplant material in veterinary and regenerative medicine, further research on cell biology, biomechanical properties of the membrane, and cell viability should be conducted.
Anti-Foulant Ultrafiltration Polymer Composite Membranes Incorporated with Composite Activated Carbon/Chitosan and Activated Carbon/Thiolated Chitosan with Enhanced Hydrophilicity
Authors: Nayab Syeda Samia , Abbas M. Asad , Mushtaq Shehla , Khan Niazi Bilal , Batool Mehwish , Shehnaz Gul , Ahmad Naveed , Ahmad Nasir M.
Published: October 2021

A rapid increase in population worldwide is giving rise to the severe problem of safe drinking water availability, necessitating the search for solutions that are effective and economical. For this purpose, membrane technology has shown a lot of promise but faces the challenge of fouling, leading to a reduction in its lifetime. In this study, ultrafiltration polyethersulfone membranes were synthesized in two different concentrations, 16% wt. and 20% wt., using the phase inversion method. Chitosan and activated carbon were incorporated as individual fillers and then as composites in both the concentrations. A novel thiolated chitosan/activated carbon composite was introduced into a polyethersulfone membrane matrix. The membranes were then analyzed using Attenuated Total Reflection–Fourier-Transform Infrared spectroscopy(ATR-FTIR), Scanning Electron Microscopy (SEM), optical profilometry, gravimetric analysis, water retention, mechanical testing and contact angle. For membranes with the novel thiolated chitosan/activated carbon composite, Scanning Electron Microscopy micrographs showed better channels, indicating a better permeability possibility, reiterated by the flux rate results. The flux rate and bovine serum albumin flux were also assessed, and the results showed an increase from 105 L/m2h to 114 L/m2h for water flux and the antifouling determined by bovine serum albumin flux increased from 23 L/m2h to 51 L/m2h. The increase in values of water uptake from 22.84% to 76.5% and decrease in contact angle from 64.5 to 55.7 showed a significant increase in the hydrophilic character of the membrane.
Special Issue: Dynamics and Nano-Organization in Plasma Membranes
Authors: Zulueta Díaz Yenisleidy de las Mercedes , Arnspang Eva Christensen
Published: October 2021

Cell membranes develop extraordinarily complex lipids and proteins geared to perform functions required by cells [...]
Effect of Alternating Magnetic Field on Arc Plasma Characteristics and Droplet Transfer during Narrow Gap Laser-MIG Hybrid Welding
Authors: Cai Baihao , Fu Juan , Zhao Yong , Chen Fugang , Qin Yonghui , Song Shuming
Published: October 2021

In this paper, the morphological characteristics of arc plasma and droplet transfer during the alternating magnetic field-assisted narrow gap groove laser-MIG (metal inert gas) hybrid welding process were investigated. The characteristics of arc plasma and droplet transfer, electron temperature, and density were analyzed using a high-speed camera and spectrum diagnosis. Our results revealed that the arc maintained a relatively stable state and rotated at a high speed to enhance the arc stiffness, and further improved the stability of the arc under the alternating magnetic field. The optimum magnetic field parameters in this experiment were B = 16 mT and f = 20 Hz, the electron temperature was 9893.6 K and the electron density was 0.99 × 1017 cm−3 near the bottom of the groove, which improved the temperature distribution inside the narrow gap groove and eliminated the lack of sidewall fusion defect. Compared to those without a magnetic field, the magnetic field could promote droplet transfer, the droplet diameter decreased by 17.6%, and the transition frequency increased by 23.5% (owing to the centrifugal force during droplet spinning and electromagnetic contraction force). The width of the weld bead was increased by 12.4% and the pores were also significantly reduced due to the stirring of the magnetic field on the molten pool.
Effects of Laser Peening with a Pulse Energy of 1.7 mJ on the Residual Stress and Fatigue Properties of A7075 Aluminum Alloy
Authors: Sano Yuji , Masaki Kiyotaka , Mizuta Yoshio , Tamaki Satoshi , Hosokai Tomonao , Taira Takunori
Published: October 2021

Laser peening without coating (LPwC) using a palmtop-sized microchip laser has improved the residual stresses (RSs) and fatigue properties of A7075 aluminum alloy. Laser pulses with a wavelength of 1.06 μm and duration of 1.3 ns from a Q-switched Nd:YAG microchip laser were focused onto A7075 aluminum alloy samples covered with water. X-ray diffraction revealed compressive RSs on the surface after irradiation using laser pulses with an energy of 1.7 mJ, spot diameter of 0.3 mm, and density of 100–1600 pulse/mm2. The effects were evident to depths of a few hundred micrometers and the maximum compressive RS was close to the yield strength. Rotation-bending fatigue experiments revealed that LPwC with a pulse energy of 1.7 mJ significantly prolonged the fatigue life and increased the fatigue strength by about 100 MPa with 107 fatigue cycles. The microchip laser used in this study is small enough to fit in the hand or be mounted on a robot arm. The results may lead to the development of tools that extend the service life of various metal parts and structures, especially outdoors where conventional lasers are difficult to apply.
Enabling Multi-Material Structures of Co-Based Superalloy Using Laser Directed Energy Deposition Additive Manufacturing
Authors: Aydogan Beytullah , Sahasrabudhe Himanshu
Published: October 2021

Cobalt superalloys such as Tribaloys are widely used in environments that involve high temperatures, corrosion, and wear degradation. Additive manufacturing (AM) processes have been investigated for fabricating Co-based alloys due to design flexibility and efficient materials usage. AM processes are suitable for reducing the manufacturing steps and subsequently reducing manufacturing costs by incorporating multi-materials. Laser directed energy deposition (laser DED) is a suitable AM process for fabricating Co-based alloys. T800 is one of the commercially available Tribaloys that is strengthened through Laves phases and of interest to diverse engineering fields. However, the high content of the Laves phase makes the alloy prone to brittle fracture. In this study, a Ni-20%Cr alloy was used to improve the fabricability of the T800 alloy via laser DED. Different mixture compositions (20%, 30%, 40% NiCr by weight) were investigated. The multi-material T800 + NiCr alloys were heat treated at two different temperatures. These alloy chemistries were characterized for their microstructural, phase, and mechanical properties in the as-fabricated and heat-treated conditions. SEM and XRD characterization indicated the stabilization of ductile phases and homogenization of the Laves phases after laser DED fabrication and heat treatment. In conclusion, the NiCr addition improved the fabricability and structural integrity of the T800 alloy.
Gut Microbiota Metabolism of Bile Acids Could Contribute to the Bariatric Surgery Improvements in Extreme Obesity
Authors: Ocaña-Wilhelmi Luis , Martín-Núñez Gracia María , Ruiz-Limón Patricia , Alcaide Juan , García-Fuentes Eduardo , Gutiérrez-Repiso Carolina , Tinahones Francisco J. , Moreno-Indias Isabel
Published: October 2021

Bariatric surgery is the only procedure to obtain and maintain weight loss in the long term, although the mechanisms driving these benefits are not completely understood. In the last years, gut microbiota has emerged as one of the drivers through its metabolites, especially secondary bile acids. In the current study, we have compared the gut microbiota and the bile acid pool, as well as anthropometric and biochemical parameters, of patient with morbid obesity who underwent bariatric surgery by two different techniques, namely Roux-en-Y gastric bypass (RYGB) or sleeve gastrectomy (SG). Gut microbiota populations differed after the respective procedures, particularly with respect to the Enterobacteriaceae family. Both techniques resulted in changes in the bile acids pool, but RYGB was the procedure which suffered the greatest changes, with a reduction in most of their levels. Blautia and Veillonella were the two genera that more relationships showed with secondary bile acids, indicating a possible role in their formation and inhibition, respectively. Correlations with the anthropometric and biochemical variables showed that secondary bile acids could have a role in the amelioration of the glucose and HDL-cholesterol levels. Thus, we have observed a possible relationship between the interaction of the bile acids pool metabolized by the gut microbiota in the metabolic improvements obtained by bariatric surgery in the frame of morbid obesity, deserving further investigation in greater cohorts to decipher the role of each bile acid in the homeostasis of the host for their possible use in the development of microbiota-based therapeutics, such as new drugs, postbiotics or probiotics.
Novel Cytoskeleton-Associated Proteins in Trypanosoma brucei Are Essential for Cell Morphogenesis and Cytokinesis
Authors: Schock Marina , Schmidt Steffen , Ersfeld Klaus
Published: October 2021

Trypanosome brucei, the causative agent of African sleeping sickness, harbours a highly ordered, subpellicular microtubule cytoskeleton that defines many aspects of morphology, motility and virulence. This array of microtubules is associated with a large number of proteins involved in its regulation. Employing proximity-dependent biotinylation assay (BioID) using the well characterised cytoskeleton-associated protein CAP5.5 as a probe, we identified CAP50 (Tb927.11.2610). This protein colocalises with the subpellicular cytoskeleton microtubules but not with the flagellum. Depletion by RNAi results in defects in cytokinesis, morphology and partial disorganisation of microtubule arrays. Published proteomics data indicate a possible association of CAP50 with two other, yet uncharacterised, cytoskeletal proteins, CAP52 (Tb927.6.5070) and CAP42 (Tb927.4.1300), which were therefore included in our analysis. We show that their depletion causes phenotypes similar to those described for CAP50 and that they are essential for cellular integrity.
Rare Earth Elements Recycling Potential Estimate Based on End-of-Life NdFeB Permanent Magnets from Mobile Phones and Hard Disk Drives in Brazil
Authors: München Daniel Dotto , Stein Ronei Tiago , Veit Hugo Marcelo
Published: October 2021

Besides neodymium, the chemical composition of Neodymium–Iron–Boron (NdFeB) permanent magnets possibly contains other rare earth elements (REEs) such as praseodymium, dysprosium, and terbium. Among its applications, NdFeB magnets are essential for Hard Disk Drives (HDDs) in computers for data storage, in Mobile Phones (MPs), and in acoustic transducers. Because REEs were classified as critical raw materials by the European Union and the USA, the recycling of them has become an important strategy to diminish supply risk. Therefore, in this publication, the authors have uncovered the recycling potential estimate (RPE) of these four REEs from both end-of-life (EoL) secondary sources. The results were based on the time-step method, using in-use stock and sales data from Brazil over the last decade (2010–2019). Moreover, the NdFeB magnets were characterized by content and weight to a more accurate RPE. The EoL generation over the decade studied showed different scenarios for MPs and HDDs, mainly due to lifespan, social behavior regarding storage and usage, and resources. Under those circumstances, the RPE revealed 211.30 t of REEs that could return as raw materials in the last decade, of which approximately 80% is neodymium. Unfortunately, recycling rates are still too low, even more so in Brazil, which is problematic for the future REE supply chain and electronic waste figures.
Atypical Mineralization Involving Pd-Pt, Au-Ag, REE, Y, Zr, Th, U, and Cl-F in the Oktyabrsky Deposit, Norilsk Complex, Russia
Authors: Barkov Andrei Y. , Nikulin Ivan I. , Nikiforov Andrey A. , Lobastov Boris M. , Silyanov Sergey A. , Martin Robert F.
Published: October 2021

Highly atypical mineralization involving Pd-Pt, Au-Ag, REE, Y, Zr, U, Th, and Cl-F-enriched minerals is found in zones with base metal sulfides (BMS; ~5 vol.% to 20 vol.%) in the eastern portion of the Oktyabrsky deposit in the Norilsk complex (Russia). The overall variations in Mg# index, 100 Mg/(Mg + Fe2+ + Mn), in host-rock minerals are 79.8 → 74.1 in olivine, 77.7 → 65.3 in orthopyroxene, 79.9 → 9.2 in clinopyroxene, and An79.0 → An3.7. The span of clinopyroxene and plagioclase compositions reflects their protracted crystallization from early magmatic to late interstitial associations. The magnesian chromite (Mg# 43.9) trends towards Cr-bearing magnetite with progressive buildups in oxygen fugacity; ilmenite varies from early Mg-rich to late Mn-rich variants. The main BMS are chalcopyrite, pyrrhotite, troilite, and Co-bearing pentlandite, with less abundant cubanite (or isocubanite), rare bornite, Co-bearing pyrite, Cd-bearing sphalerite (or wurtzite), altaite, members of the galena-clausthalite series and nickeline. A full series of Au-Ag alloy compositions is found with minor hessite, acanthite and argentopentlandite. The uncommon assemblage includes monazite-(Ce), thorite-coffinite, thorianite, uraninite, zirconolite, baddeleyite, zircon, bastnäsite-(La), and an unnamed metamict Y-dominant zirconolite-related mineral. About 20 species of PGM (platinum group minerals) were analyzed, including Pd-Pt tellurides, bismuthotellurides, bismuthides and stannides, Pd antimonides and plumbides, a Pd-Ag telluride, a Pt arsenide, a Pd-Ni arsenide, and unnamed Pd stannide-arsenide, Pd germanide-arsenide and Pt-Cu arseno-oxysulfide. The atypical assemblages are associated with Cl-rich annite with up to 7.54 wt.% Cl, Cl-rich hastingsite with up 4.06 wt.% Cl, ferro-hornblende (2.53 wt.% Cl), chlorapatite (>6 wt.% Cl) and extensive solid solutions of chlorapatite, fluorapatite and hydroxylapatite, Cl-bearing members of the chlorite group (chamosite; up to 0.96 wt.% Cl), and a Cl-bearing serpentine (up to 0.79 wt.% Cl). A decoupling of Cl and F in the geochemically evolved system is evident. The complex assemblages formed late from Cl-enriched fluids under subsolidus conditions of crystallization following extensive magmatic differentiation in the ore-bearing sequences.
Insights and Lessons from 3D Geological and Geophysical Modeling of Mineralized Terranes in Tasmania
Authors: Bombardieri Daniel , Duffett Mark , McNeill Andrew , Cracknell Matthew , Reading Anya
Published: October 2021

Over the last two decades, Mineral Resources Tasmania has been developing regional 3D geological and geophysical models for prospective terranes at a range of scales and extents as part of its suite of precompetitive geoscience products. These have evolved in conjunction with developments in 3D modeling technology over that time. Commencing with a jurisdiction-wide 3D model in 2002, subsequent modeling projects have explored a range of approaches to the development of 3D models as a vehicle for the better synthesis and understanding of controls on ore-forming processes and prospectivity. These models are built on high-quality potential field data sets. Assignment of bulk properties derived from previous well-constrained geophysical modeling and an extensive rock property database has enabled the identification of anomalous features that have been targeted for follow-up mineral exploration. An aspect of this effort has been the generation of uncertainty estimates for model features. Our experience is that this process can be hindered by models that are too large or too detailed to be interrogated easily, especially when modeling techniques do not readily permit significant geometric changes. The most effective 3D modeling workflow for insights into mineral exploration is that which facilitates the rapid hypothesis testing of a wide range of scenarios whilst satisfying the constraints of observed data.
Sequential Injection System for Analysis of Degree Brix, Orthophosphate and pH in Raw Sugarcane Juice Applicable to Sugar Industry
Authors: Saetear Phoonthawee , Saechua Nattinee , Sereenonchai Kamonthip
Published: October 2021

This work presents, for the first time, a new sequential injection analysis (SIA) method to simultaneously analyze degree Brix, orthophosphate and pH in raw cane juice. These key parameters relate to price of harvested sugarcane and quality of cane juice for sugar production. The SIA system employed two detectors: the first detector is a diode-array spectrophotometer, equipped with a regular flow cell, for measurements of degree Brix and orthophosphate. Quantitative of degree Brix (°Bx; ca. % (w/w) sucrose) was based on manipulation of the schlieren effect at the interface between plugs of sample and water. Orthophosphate analysis was carried out based on the molybdenum blue method with significant reduction in consumption of the reagents. Compensation of the schlieren effect from sucrose for determination of orthophosphate was achieved by using a dual-wavelength spectrometric detection. Second detector is a pH-sensing device, called ion-selective field-effect transistors (ISFET). The ISFET is based on the current through the ISFET arising according to the H+ concentration in solution. Our developed SIA system provides linear calibration graphs fitting for purpose in analysis of sugarcane juice (pH: 0–14, °Bx: 1.0–7.0 and P2O5: 20–200 mg L−1). Simultaneous analysis of sugarcane juice for pH, °Bx and P2O5 is carried out within 5 min (12 sample per h). Precision of SIA system is acceptable (RSD < 3%). Our SIA system gave quantitative results insignificantly different, as compared with conventional methods for analysis of pH, °Bx and P2O5 in sugarcane juice.
An Insight into Geometries and Catalytic Applications of CeO2 from a DFT Outlook
Authors: Miran Hussein A. , Jaf Zainab N. , Altarawneh Mohammednoor , Jiang Zhong-Tao
Published: October 2021

Rare earth metal oxides (REMOs) have gained considerable attention in recent years owing to their distinctive properties and potential applications in electronic devices and catalysts. Particularly, cerium dioxide (CeO2), also known as ceria, has emerged as an interesting material in a wide variety of industrial, technological, and medical applications. Ceria can be synthesized with various morphologies, including rods, cubes, wires, tubes, and spheres. This comprehensive review offers valuable perceptions into the crystal structure, fundamental properties, and reaction mechanisms that govern the well-established surface-assisted reactions over ceria. The activity, selectivity, and stability of ceria, either as a stand-alone catalyst or as supports for other metals, are frequently ascribed to its strong interactions with the adsorbates and its facile redox cycle. Doping of ceria with transition metals is a common strategy to modify the characteristics and to fine-tune its reactive properties. DFT-derived chemical mechanisms are surveyed and presented in light of pertinent experimental findings. Finally, the effect of surface termination on catalysis by ceria is also highlighted.
Molecular Dynamics Simulations Study of the Interactions between Human Dipeptidyl-Peptidase III and Two Substrates
Authors: Zhang Shitao , Lv Shuai , Fu Xueqi , Han Lu , Han Weiwei , Li Wannan
Published: October 2021

Human dipeptidyl-peptidase III (hDPP III) is capable of specifically cleaving dipeptides from the N-terminal of small peptides with biological activity such as angiotensin II (Ang II, DRVYIHPF), and participates in blood pressure regulation, pain modulation, and the development of cancers in human biological activities. In this study, 500 ns molecular dynamics simulations were performed on free-hDPP III (PDB code: 5E33), hDPP III-Ang II (PDB code: 5E2Q), and hDPP III-IVYPW (PDB code: 5E3C) to explore how these two peptides affect the catalytic efficiency of enzymes in terms of the binding mode and the conformational changes. Our results indicate that in the case of the hDPP III-Ang II complex, subsite S1 became small and hydrophobic, which might be propitious for the nucleophile to attack the substrate. The structures of the most stable conformations of the three systems revealed that Arg421-Lys423 could form an α-helix with the presence of Ang II, but only part of the α-helix was produced in hDPP III-IVYPW. As the hinge structure in hDPP III, the conformational changes that took place in the Arg421-Lys423 residue could lead to the changes in the shape and space of the catalytic subsites, which might allow water to function as a nucleophile to attack the substrate. Our results may provide new clues to enable the design of new inhibitors for hDPP III in the future.
Study on the Dynamic Difference between Single and Mixed Residues of Three Neonicotinoids in Brassica chinensis L.
Authors: Lu Yangyang , Rao Qinxiong , Zhang Qicai , Liu Xing , Song Wei , Guan Shuhui , Chen Shanshan , Song Weiguo
Published: October 2021

Multiple insecticides’ residues after the mixed application of several neonicotinoids cause combined pollution and bring new challenges to food safety and pest control during agricultural production. In this study, three neonicotinoid insecticides, namely imidacloprid (IMI), acetamiprid (ACE), and thiamethoxam (TMX), were mixed and evenly sprayed on Brassica chinensis L. in the field. Then, the insecticides’ residues were dynamically monitored to determine the differences in their rates of dissipation and final residues after 10 days. The results showed that the dissipation kinetics of neonicotinoids still conformed to the first-order kinetic model for binary or ternary application of neonicotinoid mixtures, with all determination coefficients (R2) being above 0.9 and the dissipation half-life (DT50) being 2.87–6.74 d. For treatment groups with five times the recommended dosages (IMI 300 g·hm−2, ACE 900 g·hm−2, and TMX 600 g·hm−2), mixed insecticides had a slower dissipation rate, and the DT50 values of mixtures were longer than those of single insecticides. Moreover, the final insecticide residues with mixed application were higher than those of single compounds at 10 d after spraying. Thus, mixed applications of neonicotinoids may increase food safety risks as they increase the final insecticide residues in Brassica chinensis L., and care should therefore be taken when considering the combined use of such compounds.
Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage
Authors: Bozhokin Mikhail S. , Bozhkova Svetlana A. , Rubel Aleksandr A. , Sopova Julia V. , Nashchekina Yulia A. , Bildyug Natalya B. , Khotin Mikhail G.
Published: October 2021

Damage to the hyaline layer of the articular surface is an urgent problem for millions of people around the world. At present, a large number of experimental methods are being developed to address this problem, including the transplantation of a cell-engineered construct (CEC) composed of a biodegradable scaffold with a premixed cell culture into the damaged area of the articular surface. However, current methods for analyzing the effectiveness of such CECs have significant limitations. This study aimed to compare the SEM technique, classical histology, and cryosectioning for the analysis of CECs transplanted to hyaline cartilage.
Study of the Role of Void and Residual Silicon Dioxide on the Electrochemical Performance of Silicon Nanoparticles Encapsulated by Graphene
Authors: Argyropoulos Dimitrios-Panagiotis , Zardalidis George , Giotakos Panagiotis , Daletou Maria , Farmakis Filippos
Published: October 2021

Silicon nanoparticles are used to enhance the anode specific capacity for the lithium-ion cell technology. Due to the mechanical deficiencies of silicon during lithiation and delithiation, one of the many strategies that have been proposed consists of enwrapping the silicon nanoparticles with graphene and creating a void area between them so as to accommodate the large volume changes that occur in the silicon nanoparticle. This work aims to investigate the electrochemical performance and the associated kinetics of the hollow outer shell nanoparticles. To this end, we prepared hollow outer shell silicon nanoparticles (nps) enwrapped with graphene by using thermally grown silicon dioxide as a sacrificial layer, ball milling to enwrap silicon particles with graphene and hydro fluorine (HF) to etch the sacrificial SiO2 layer. In addition, in order to offer a wider vision on the electrochemical behavior of the hollow outer shell Si nps, we also prepared all the possible in-between process stages of nps and corresponding electrodes (i.e., bare Si nps, bare Si nps enwrapped with graphene, Si/SiO2 nps and Si/SiO2 nps enwrapped with graphene). The morphology of all particles revealed the existence of graphene encapsulation, void, and a residual layer of silicon dioxide depending on the process of each nanoparticle. Corresponding electrodes were prepared and studied in half cell configurations by means of galvanostatic cycling, cyclic voltammetry and electrochemical impedance spectroscopy. It was observed that nanoparticles encapsulated with graphene demonstrated high specific capacity but limited cycle life. In contrast, nanoparticles with void and/or SiO2 were able to deliver improved cycle life. It is suggested that the existence of the void and/or residual SiO2 layer limits the formation of rich LiXSi alloys in the core silicon nanoparticle, providing higher mechanical stability during the lithiation and delithiation processes.
Chemically Cross-Linked Graphene Oxide as a Selective Layer on Electrospun Polyvinyl Alcohol Nanofiber Membrane for Nanofiltration Application
Authors: Park Myoung Jun , Nisola Grace M. , Seo Dong Han , Wang Chen , Phuntsho Sherub , Choo Youngwoo , Chung Wook-Jin , Shon Ho Kyong
Published: October 2021

Graphene oxide (GO) nanosheets were utilized as a selective layer on a highly porous polyvinyl alcohol (PVA) nanofiber support via a pressure-assisted self-assembly technique to synthesize composite nanofiltration membranes. The GO layer was rendered stable by cross-linking the nanosheets (GO-to-GO) and by linking them onto the support surface (GO-to-PVA) using glutaraldehyde (GA). The amounts of GO and GA deposited on the PVA substrate were varied to determine the optimum nanofiltration membrane both in terms of water flux and salt rejection performances. The successful GA cross-linking of GO interlayers and GO-PVA via acetalization was confirmed by FTIR and XPS analyses, which corroborated with other characterization results from contact angle and zeta potential measurements. Morphologies of the most effective membrane (CGOPVA-50) featured a defect-free GA cross-linked GO layer with a thickness of ~67 nm. The best solute rejections of the CGOPVA-50 membrane were 91.01% for Na2SO4 (20 mM), 98.12% for Eosin Y (10 mg/L), 76.92% for Methylene blue (10 mg/L), and 49.62% for NaCl (20 mM). These findings may provide one of the promising approaches in synthesizing mechanically stable GO-based thin-film composite membranes that are effective for solute separation via nanofiltration.
Simulating the Self-Assembly and Hysteresis Loops of Ferromagnetic Nanoparticles with Sticking of Ligands
Authors: Anderson Nicholas R. , Davidson Jonathon , Louie Dana R. , Serantes David , Livesey Karen L.
Published: October 2021

The agglomeration of ferromagnetic nanoparticles in a fluid is studied using nanoparticle-level Langevin dynamics simulations. The simulations have interdigitation and bridging between ligand coatings included using a computationally-cheap, phenomenological sticking parameter c. The interactions between ligand coatings are shown in this preliminary study to be important in determining the shapes of agglomerates that form. A critical size for the sticking parameter is estimated analytically and via the simulations and indicates where particle agglomerates transition from well-ordered (c is small) to disordered (c is large) shapes. Results are also presented for the hysteresis loops (magnetization versus applied field) for these particle systems in an oscillating magnetic field appropriate for hyperthermia applications. The results show that the clumping of particles has a significant effect on their macroscopic properties, with important consequences on applications. In particular, the work done by an oscillating field on the system has a nonmonotonic dependence on c.
Unique Habitual Food Intakes in the Gut Microbiota Cluster Associated with Type 2 Diabetes Mellitus
Authors: Kondo Yuriko , Hashimoto Yoshitaka , Hamaguchi Masahide , Ando Shinto , Kaji Ayumi , Sakai Ryosuke , Inoue Ryo , Kashiwagi Saori , Mizushima Katsura , Uchiyama Kazuhiko , Takagi Tomohisa , Naito Yuji , Fukui Michiaki
Published: October 2021

This cross-sectional study aimed to clarify the characteristic gut microbiota of Japanese patients with type 2 diabetes (T2DM) using t-distributed stochastic neighbor embedding analysis and the k-means method and to clarify the relationship with background data, including dietary habits. The gut microbiota data of 383 patients with T2DM and 114 individuals without T2DM were classified into red, blue, green, and yellow groups. The proportions of patients with T2DM in the red, blue, green, and yellow groups was 86.8% (112/129), 69.8% (81/116), 76.3% (90/118), and 74.6% (100/134), respectively; the red group had the highest prevalence of T2DM. There were no intergroup differences in sex, age, or body mass index. The red group had higher percentages of the Bifidobacterium and Lactobacillus genera and lower percentages of the Blautia and Phascolarctobacterium genera. Higher proportions of patients with T2DM in the red group used α-glucosidase inhibitors and glinide medications and had a low intake of fermented soybean foods, including miso soup, than those in the other groups. The gut microbiota pattern of the red group may indicate characteristic changes in the gut microbiota associated with T2DM in Japan. These results also suggest that certain diabetes drugs and fermented foods may be involved in this change. Further studies are needed to confirm the relationships among traditional dietary habits, the gut microbiota, and T2DM in Japan.
Effects of Sarcopenic Obesity and Its Confounders on Knee Range of Motion Outcome after Total Knee Replacement in Older Adults with Knee Osteoarthritis: A Retrospective Study
Authors: Liao Chun-De , Huang Shih-Wei , Huang Yu-Yun , Lin Che-Li
Published: October 2021

Sarcopenic obesity is closely associated with knee osteoarthritis (KOA) and has high risk of total knee replacement (TKR). In addition, poor nutrition status may lead to sarcopenia and physical frailty in KOA and is negatively associated with surgery outcome after TKR. This study investigated the effects of sarcopenic obesity and its confounding factors on recovery in range of motion (ROM) after total knee replacement (TKR) in older adults with KOA. A total of 587 older adults, aged ≥60 years, who had a diagnosis of KOA and underwent TKR, were enrolled in this retrospective cohort study. Sarcopenia and obesity were defined based on cutoff values of appendicular mass index and body mass index for Asian people. Based on the sarcopenia and obesity definitions, patients were classified into three body-composition groups before TKR: sarcopenic-obese, obese, and non-obese. All patients were asked to attend postoperative outpatient follow-up admissions. Knee flexion ROM was measured before and after surgery. A ROM cutoff of 125 degrees was used to identify poor recovery post-surgery. Kaplan-Meier curve analysis was performed to measure the probability of poor ROM recovery among study groups. Cox multivariate regression models were established to calculate the hazard ratios (HRs) of postoperative poor ROM recovery, using potential confounding factors including age, sex, comorbidity, risk of malnutrition, preoperative ROM, and outpatient follow-up duration as covariates. Analyses results showed that patients in the obese and sarcopenic-obese groups had a higher probability of poor ROM recovery compared to the non-obese group (all p < 0.001). Among all body-composition groups, the sarcopenic-obese group yielded the highest risk of postoperative physical difficulty (adjusted HR = 1.63, p = 0.03), independent to the potential confounding factors. Sarcopenic obesity is likely at the high risk of poor ROM outcome following TKR in older individuals with KOA.