bluuebunny/crossref_metadata_2025 huggingface dataset:
Pre-electoral coalitions and voter turnout
Authors: Tillman Erik R
Published: September 2013

Research on comparative voter turnout has produced a puzzling set of findings: proportional representation (PR) electoral systems increase turnout, but multiparty systems decrease turnout. This paper provides new evidence to resolve these conflicting findings by developing and testing the hypothesis that the presence of a pre-electoral coalition (PEC) increases voter turnout by reducing uncertainty about the possible government that will form after the election. PECs provide credible signals to voters about the commitment of participating parties to govern together, making the election more decisive as a mechanism for selecting the government by clarifying the possible electoral outcomes. This increased electoral decisiveness increases voters' incentives to turn out. This hypothesis is tested on a data set of 223 national legislative elections in 19 parliamentary democracies between 1970 and 2002, with the results indicating that the presence of a PEC increases turnout on average by more than 1.5 percentage points. This finding provides support for the argument that the electoral context and strategic party behavior have important effects on voter participation.
Participants’ experiences of facilitated physical activity for the management of depression in primary care
Authors: Searle Aidan , Haase Anne M , Chalder Melanie , Fox Kenneth R , Taylor Adrian H , Lewis Glyn , Turner Katrina M
Published: September 2013

A qualitative study was conducted within a randomised trial of facilitated physical activity for depression based on Self-Determination Theory and motivational interviewing. Interviews were held with 19 participants at 4 months, and 12 participants were re-interviewed 8 months later. The interviews were analysed in accordance with Grounded Theory using framework. Themes consisted of the following: relationship with the physical activity facilitators, mode of facilitation, impact of contact with physical activity facilitator/assimilation and future plans, change in activity, and effectiveness of physical activity facilitator techniques. Engagement in physical activity was enhanced within an autonomy-supportive environment.
“Miguel es un artista”—and Luisa is an excellent student: Seeking time and space for children’s multimodal practices
Authors: Falchi Lorraine T , Axelrod Ysaaca , Genishi Celia
Published: September 2013

This paper draws data from a 5-year ethnographic study of young multilingual (Mixteco/Spanish/English) children in their early childhood classrooms. In this paper, we focus on two of the children and their distinctive paths as they develop language and literacy. Using a sociocultural and multimodal theoretical framework we examine how these two children participate in and negotiate a balanced literacy curriculum.
For and against the four principles of biomedical ethics
Authors: Huxtable Richard
Published: September 2013

The four principles approach to biomedical ethics points to respect for autonomy, beneficence, non-maleficence and justice as the norms that should guide moral agents working in the biosciences, and particularly in health care. While the approach is well known, it is not without its critics. In this paper, which is primarily aimed at health professionals and students (from various disciplines) who are studying health care ethics, I consider four problems with the four principles, which respectively claim that the approach is imperialist, inapplicable, inconsistent and inadequate. In keeping with the aims of the ‘five-minute focus’, the primary objective is to introduce these debates, rather than seek to resolve them. However, I will suggest that the approach does have its merits, not least for time-pressed clinicians who are keen to keep an eye on the ethical dimensions of their practices, and for students training in the health care professions, provided that they appreciate that the approach provides only a starting point for, and not the end point of, moral deliberation.
Is the debate about single or double embryo transfer following in vitro fertilisation really an ethical dilemma?
Authors: Jean Miguel , Tessier Philippe , Bonnaud-Antignac Angélique , Freour Thomas , Barriere Paul , Dabouis Gérard
Published: September 2013

In vitro fertilisation (IVF) daily practice reveals that couples are willing to take greater risks than doctors if there is a higher chance of pregnancy. Arising from this is a frequently addressed issue regarding the embryo transfer strategy: single or double embryo transfer? The dilemma is faced by patients, as well as physicians, who are caught between the possibility of no pregnancies at all and facing the prospect of iatrogenic twin gestation.

How could the couple's preferences concerning how many children they would like be taken into account in determining the number of embryos transferred? How should the physician exercise his medical responsibility towards his patients and the unborn child? An effective approach could be to enhance a couple’s autonomy by advocating a medical beneficence that incorporates patients’ values. This can be achieved through a case-by-case approach in a deliberative process of decision-making which includes real patient-centred communication.
Parent–Child Interaction Therapy With Deaf Parents and Their Hearing Child
Authors: Armstrong Kathleen , David Amanda , Goldberg Karen
Published: September 2013

There are few proven effective treatments such as Parent–Child Interaction Therapy (PCIT) for use with deaf parents and their children, even though it is likely that the prevalence rate for disruptive behavior problems including attention deficit/hyperactivity disorder (ADHD) and oppositional defiant disorder (ODD) are similar to those reported for all U.S. children. Medication and behavioral therapy involving parents comprise the cornerstone for ADHD/ODD management, with PCIT endorsed as one of the most effective interventions available for children aged 2 to 7 years. This case study presents the implementation of PCIT with deaf parents and their 7-year-old hearing son with ADHD/ODD, with the help of a certified interpreter and readily available video technology. Findings from this case report documented PCIT as a promising treatment option for use with individuals who are deaf.
Effect of membrane ballooning on screen pressure equalization: A short literature review
Authors: Shi Xing , Burnett Eric
Published: September 2013

In residential buildings, a typical rainscreen exterior wall system often incorporates an exterior membrane mechanically fastened to an exterior sheathing. This thin and flexible membrane is an important component of the wall system and performs multiple functions. Under a negative air pressure differential, that is, wind suction, the exterior membrane can deform or balloon. The membrane ballooning has important implications on many performance issues in the rainscreen exterior wall system. That the membrane ballooning changes the air cavity volume and therefore affects rainscreen pressure equalization is one important implication. Since the 1980s, many theoretical pressure equalization models have been proposed. This article takes a unique viewpoint and focuses on reviewing how the theoretical screen pressure equalization models consider the effect of membrane ballooning. It is believed that such a review is essential for future study on how to incorporate the membrane ballooning into the pressure equalization model.
Minority Stress and Physical Health Among Sexual Minorities
Authors: Lick David J. , Durso Laura E. , Johnson Kerri L.
Published: September 2013

Lesbian, gay, and bisexual (LGB) individuals suffer serious mental health disparities relative to their heterosexual peers, and researchers have linked these disparities to difficult social experiences (e.g., antigay victimization) and internalized biases (e.g., internalized homophobia) that arouse stress. A recent and growing body of evidence suggests that LGB individuals also suffer physical health disparities relative to heterosexuals, ranging from poor general health status to increased risk for cancer and heightened diagnoses of cardiovascular disease, asthma, diabetes, and other chronic conditions. Despite recent advances in this literature, the causes of LGB physical health problems remain relatively opaque. In this article, we review empirical findings related to LGB physical health disparities and argue that such disparities are related to the experience of minority stress—that is, stress caused by experiences with antigay stigma. In light of this minority stress model, we highlight gaps in the current literature and outline five research steps necessary for developing a comprehensive knowledge of the social determinants of LGB physical health.
A Bird’s Eye View of Unethical Behavior
Authors: Ariely Dan , Mann Heather
Published: September 2013

Results from Trautmann and colleagues’ large, representative survey of Dutch people suggest a more nuanced relationship between class and ethics than previous research has demonstrated (Trautmann, Van de Kuilen, & Zeckhauser, 2013, this issue). Following their analysis, we suggest that it is unlikely that either upper- or lower-class people are unequivocally more moral. Rather, several psychological and external forces are at play in ethical decision making, which likely vary in strength depending on the conceptualization of class and the sociocultural context. Furthermore, people from different social classes may have different ethical standards or different degrees of willingness to breach these standards (or both), a distinction that should be explored in future research.
Multiple Chronic Conditions
Authors: Fisher Monica A. , Ma Zhen-qiang
Published: September 2013

Background: Multiple chronic conditions (MCCs) are significant public health problems receiving much attention, and rightly so, because of the substantial morbidity, mortality, and cost burden. Objectives: To report the prevalence of selected frequent MCCs with shared risk factors that are leading causes of death; and to quantify the association between diabetes and its comorbidity and shared risk factors. Methods: This is a cross-sectional study of 9172 participants in Pennsylvania’s 2009 Behavioral Risk Factor Surveillance System (BRFSS). Centers for Disease Control and Prevention’s Web Enabled Analysis Tool (WEAT) and SAS Proc Survey were used for all analyses, to account for BRFSS’s multistage complex survey design and sample weights. Results: SAS estimated prevalence among ≥35-year-olds were as follows: diabetes (11.9%), heart disease (9.5%), stroke (3.3%), diabetes and heart disease (2.9%), diabetes and stroke (1.0%), heart disease and stroke (1.1%), and diabetes, heart disease, and stroke (0.4%). Adults ≥35 years old with the following characteristics were more likely to have diabetes when self-reporting: obese (adjusted odds ratio [ORAdj] = 4.63, 95% confidence interval [95% CI] = 3.41-6.30); low income (<$15 000 ORAdj = 2.85, 95% CI = 1.90-4.27; $15 000-$24 999 ORAdj = 2.07, 95% CI = 1.50-2.86; $25 000-$34 999 ORAdj = 2.06, 95% CI = 1.47-2.88; $35 000-$49 999 ORAdj = 1.51, 95% CI = 1.08-2.12); elderly ≥65 years old (ORAdj = 2.84, 95% CI = 1.76-4.56); non-Hispanic black (ORAdj = 2.54, 95% CI = 1.08-6.00); overweight (ORAdj = 1.99, 95% CI = 1.47-2.68); heart disease (ORAdj = 1.94, 95% CI = 1.47-2.56); 45 to 64 years old (ORAdj = 1.88 95%, CI = 1.21-2.93); hypertension (ORAdj = 1.78, 95% CI = 1.39-2.29); stroke (ORAdj = 1.62, 95% CI = 1.05-2.50); high cholesterol (ORAdj = 1.49, 95% CI = 1.18-1.88); physically inactive (ORAdj = 1.33 95% CI = 1.06-1.67); and men (ORAdj = 1.32, 95% CI = 1.04-1.67). Conclusion: Adults with comorbidity and risk factors were 1.3 to 4.6 times more likely to have diabetes, with obesity consistently the strongest risk factor. WEAT’s limitations are the predefined categories, inability to restrict to specific age groups and to estimate the prevalence of >2 MCCs. WEAT has considerable potential to advance evidence-based primary care practice and community health programs, such as state-wide programs that target obesity and physical inactivity. The robust capability of SAS to calculate MCC prevalence and adjusted associations with risk factors provides the fundamentals for developing and evaluating primary care services and community health programs.
BLT-humanized C57BL/6 Rag2−/−γc−/−CD47−/− mice are resistant to GVHD and develop B- and T-cell immunity to HIV infection
Authors: Lavender Kerry J. , Pang Wendy W. , Messer Ronald J. , Duley Amanda K. , Race Brent , Phillips Katie , Scott Dana , Peterson Karin E. , Chan Charles K. , Dittmer Ulf , Dudek Timothy , Allen Todd M. , Weissman Irving L. , Hasenkrug Kim J.
Published: September 2013

Key Points

Tolerance induced in the absence of CD47 signaling. C57BL/6 Rag2−/−γc−/−CD47−/− mice humanized without GVHD.
GATA1-mutant clones are frequent and often unsuspected in babies with Down syndrome: identification of a population at risk of leukemia
Authors: Roberts Irene , Alford Kate , Hall Georgina , Juban Gaetan , Richmond Helen , Norton Alice , Vallance Grant , Perkins Kelly , Marchi Emanuele , McGowan Simon , Roy Anindita , Cowan Gillian , Anthony Mark , Gupta Amit , Ho John , Uthaya Sabita , Curley Anna , Rasiah Shree Vishna , Watts Timothy , Nicholl Richard , Bedford-Russell Alison , Blumberg Raoul , Thomas Angela , Gibson Brenda , Halsey Chris , Lee Pek-Wan , Godambe Sunit , Sweeney Connor , Bhatnagar Neha , Goriely Anne , Campbell Peter , Vyas Paresh
Published: September 2013

Key Points

GATA1 mutations are common in neonates with Down syndrome but are often unsuspected and detectable only with sensitive methods. Multilineage blood abnormalities in all Down syndrome neonates in the absence of GATA1 mutations suggests that trisomy 21 itself perturbs hemopoiesis.
Transcriptome analysis of two buffalograss cultivars
Authors: Wachholtz Michael , Heng-Moss Tiffany , Twigg Paul , Baird Lisa , Lu Guoqing , Amundsen Keenan
Published: September 2013

Background

Buffalograss [Buchloë dactyloides (Nutt.) Engel. syn. Bouteloua dactyloides (Nutt.) Columbus] is a United States native turfgrass species that requires less irrigation, fungicides and pesticides compared to more commonly used turfgrass species. In areas where water is limited, interest in this grass species for lawns is increasing. While several buffalograss cultivars have been developed through buffalograss breeding, the timeframe for new cultivar development is long and is limited by a lack of useful genetic resources. Two high throughput next-generation sequencing techniques were used to increase the genomic resources available for buffalograss.

Results

Total RNA was extracted and purified from leaf samples of two buffalograss cultivars. ‘378’ and ‘Prestige’ cDNA libraries were subjected to high throughput sequencing on the Illumina GA and Roche 454 Titanium FLX sequencing platforms. The 454 platform (3 samples) produced 1,300,885 reads and the Illumina platform (12 samples) generated approximately 332 million reads. The multiple k-mer technique for de novo assembly using Velvet and Oases was applied. A total of 121,288 contigs were assembled that were similar to previously reported Ensembl commelinid sequences. Original Illumina reads were also mapped to the high quality assembly to estimate expression levels of buffalograss transcripts. There were a total of 325 differentially expressed genes between the two buffalograss cultivars. A glycosyl transferase, serine threonine kinase, and nb-arc domain containing transcripts were among those differentially expressed between the two cultivars. These genes have been previously implicated in defense response pathways and may in part explain some of the performance differences between ‘Prestige’ and ‘378’.

Conclusions

To date, this is the first high throughput sequencing experiment conducted on buffalograss. In total, 121,288 high quality transcripts were assembled, significantly expanding the limited genetic resources available for buffalograss genetic studies. Additionally, 325 differentially expressed sequences were identified which may contribute to performance or morphological differences between ‘Prestige’ and ‘378’ buffalograss cultivars.
Microspheres targeted with a mesothelin antibody and loaded with doxorubicin reduce tumor volume of human mesotheliomas in xenografts
Authors: Macura Sherrill L , Steinbacher Jeremy L , MacPherson Maximilian B , Lathrop Melissa J , Sayan Mutlay , Hillegass Jedd M , Beuschel Stacie L , Perkins Timothy N , Spiess Page C , van der Vliet Albert , Butnor Kelly J , Shukla Arti , Wadsworth Marilyn , Landry Christopher C , Mossman Brooke T
Published: September 2013

Background

Malignant mesotheliomas (MMs) are chemoresistant tumors related to exposure to asbestos fibers. The long latency period of MM (30-40 yrs) and heterogeneity of tumor presentation make MM difficult to diagnose and treat at early stages. Currently approved second-line treatments following surgical resection of MMs include a combination of cisplatin or carboplatin (delivered systemically) and pemetrexed, a folate inhibitor, with or without subsequent radiation. The systemic toxicities of these treatments emphasize the need for more effective, localized treatment regimens.

Methods

Acid-prepared mesoporous silica (APMS) microparticles were loaded with doxorubicin (DOX) and modified externally with a mesothelin (MB) specific antibody before repeated intraperitoneal (IP) injections into a mouse xenograft model of human peritoneal MM. The health/weight of mice, tumor volume/weight, tumor necrosis and cell proliferation were evaluated in tumor-bearing mice receiving saline, DOX high (0.2 mg/kg), DOX low (0.05 mg/kg), APMS-MB, or APMS-MB-DOX (0.05 mg/kg) in saline.

Results

Targeted therapy (APMS-MB-DOX at 0.05 mg/kg) was more effective than DOX low (0.05 mg/kg) and less toxic than treatment with DOX high (0.2 mg/kg). It also resulted in the reduction of tumor volume without loss of animal health and weight, and significantly decreased tumor cell proliferation. High pressure liquid chromatography (HPLC) of tumor tissue confirmed that APMS-MB-DOX particles delivered DOX to target tissue.

Conclusions

Data suggest that targeted therapy results in greater chemotherapeutic efficacy with fewer adverse side effects than administration of DOX alone. Targeted microparticles are an attractive option for localized drug delivery.
Pain management policies and practices in pediatric emergency care: a nationwide survey of Italian hospitals
Authors: Ferrante Pierpaolo , Cuttini Marina , Zangardi Tiziana , Tomasello Caterina , Messi Gianni , Pirozzi Nicola , Losacco Valentina , Piga Simone , Benini Franca ,   the PIPER Study Group
Published: September 2013

Background

Pain experienced by children in emergency departments (EDs) is often poorly assessed and treated. Although local protocols and strategies are important to ensure appropriate staff behaviours, few studies have focussed on pain management policies at hospital or department level. This study aimed at describing the policies and reported practices of pain assessment and treatment in a national sample of Italian pediatric EDs, and identifying the assocoated structural and organisational factors.

Methods

A structured questionnaire was mailed to all the 14 Italian pediatric and maternal and child hospitals and to 5 general hospitals with separate pediatric emergency room. There were no refusals. Information collected included the frequency and mode of pain assessment, presence of written pain management protocols, use of local anaesthetic (EMLA cream) before venipuncture, and role of parents. General data on the hospital and ED were also recorded. Multiple Correspondence Analysis was used to explore the multivariable associations between the characteristics of hospitals and EDs and their pain management policies and practices.

Results

Routine pain assessment both at triage and in the emergency room was carried out only by 26% of surveyed EDs. About one third did not use algometric scales, and almost half (47.4%) did not have local protocols for pain treatment. Only 3 routinely reassessed pain after treatment, and only 2 used EMLA. All EDs allowed parents’ presence and most (17, 89.9%) allowed them to stay when painful procedures were carried out. Eleven hospitals (57.9%) allowed parents to hold their child during blood sampling. Pediatric and maternal and child hospitals, those located in the North of Italy, equipped with medico-surgical-traumatological ED and short stay observation, and providing full assessment triage over 24 hours were more likely to report appropriate policies for pain management both at triage and in ER. A nurses to admissions ratio ≥ median was associated with better pain management at triage.

Conclusions

Despite availability of national and international guidelines, pediatric pain management is still sub-optimal in Italian emergency departments. Multifaceted strategies including development of local policies, staff educational programs, and parental involvement in pain assessment should be carried out and periodically reinforced.
Biochemical and immunological mechanisms by which sickle cell trait protects against malaria
Authors: Gong Lauren , Parikh Sunil , Rosenthal Philip J , Greenhouse Bryan
Published: September 2013

Sickle cell trait (HbAS) is the best-characterized genetic polymorphism known to protect against falciparum malaria. Although the protective effect of HbAS against malaria is well known, the mechanism(s) of protection remain unclear. A number of biochemical and immune-mediated mechanisms have been proposed, and it is likely that multiple complex mechanisms are responsible for the observed protection. Increased evidence for an immune component of protection as well as novel mechanisms, such as enhanced tolerance to disease mediated by HO-1 and reduced parasitic growth due to translocation of host micro-RNA into the parasite, have recently been described. A better understanding of relevant mechanisms will provide valuable insight into the host-parasite relationship, including the role of the host immune system in protection against malaria.
The effects of increased fluid viscosity on swallowing sounds in healthy adults
Authors: Jestrović Iva , Dudik Joshua M , Luan Bo , Coyle James L , Sejdić Ervin
Published: September 2013

Background

Cervical auscultation (CA) is an affordable, non-invasive technique used to observe sounds occurring during swallowing. CA involves swallowing characterization via stethoscopes or microphones, while accelerometers can detect other vibratory signals. While the effects of fluid viscosity on swallowing accelerometry signals is well understood, there are still open questions about these effects on swallowing sounds. Therefore, this study investigated the influence of fluids with increasing thickness on swallowing sound characteristics.

Method

We collected swallowing sounds and swallowing accelerometry signals from 56 healthy participants. Each participant completed five water swallows, five swallows of nectar-thick apple juice, and five swallows of honey-thick apple juice. These swallows were completed in neutral head and chin-tuck head positions. After pre-processing of collected signals, a number of features in time, frequency and time-frequency domains were extracted.

Results

Our numerical analysis demonstrated that significant influence of viscosity was found in most of the features. In general, features extracted from swallows in the neutral head position were affected more than swallows from the chin-tuck position. Furthermore, most of the differences were found between water and fluids with higher viscosity. Almost no significant differences were found between swallows involving nectar-thick and honey-thick apple juices. Our results also showed that thicker fluids had higher acoustic regularity and predictability as demonstrated by the information-theoretic features, and a lower frequency content as demonstrated by features in the frequency domain.

Conclusions

According to these results, we can conclude that viscosity of fluids should be considered in future investigations involving swallowing sounds.
Oncolytic viruses as therapeutic cancer vaccines
Authors: Bartlett David L , Liu Zuqiang , Sathaiah Magesh , Ravindranathan Roshni , Guo Zongbi , He Yukai , Guo Zong Sheng
Published: September 2013

Oncolytic viruses (OVs) are tumor-selective, multi-mechanistic antitumor agents. They kill infected cancer and associated endothelial cells via direct oncolysis, and uninfected cells via tumor vasculature targeting and bystander effect. Multimodal immunogenic cell death (ICD) together with autophagy often induced by OVs not only presents potent danger signals to dendritic cells but also efficiently cross-present tumor-associated antigens from cancer cells to dendritic cells to T cells to induce adaptive antitumor immunity. With this favorable immune backdrop, genetic engineering of OVs and rational combinations further potentiate OVs as cancer vaccines. OVs armed with GM-CSF (such as T-VEC and Pexa-Vec) or other immunostimulatory genes, induce potent anti-tumor immunity in both animal models and human patients. Combination with other immunotherapy regimens improve overall therapeutic efficacy. Coadministration with a HDAC inhibitor inhibits innate immunity transiently to promote infection and spread of OVs, and significantly enhances anti-tumor immunity and improves the therapeutic index. Local administration or OV mediated-expression of ligands for Toll-like receptors can rescue the function of tumor-infiltrating CD8+T cells inhibited by the immunosuppressive tumor microenvironment and thus enhances the antitumor effect. Combination with cyclophosphamide further induces ICD, depletes Treg, and thus potentiates antitumor immunity. In summary, OVs properly armed or in rational combinations are potent therapeutic cancer vaccines.
The prevalence and correlates of sitting in European adults - a comparison of 32 Eurobarometer-participating countries
Authors: Bennie Jason A , Chau Josephine Y , van der Ploeg Hidde P , Stamatakis Emmanuel , Do Anna , Bauman Adrian
Published: September 2013

Background

Prolonged sitting is an emerging health risk. However, multi-country comparative sitting data are sparse. This paper reports the prevalence and correlates of sitting time in 32 European countries.

Methods

Data from the Eurobarometer 64.3 study were used, which included nationally representative samples (n = 304-1,102) from 32 European countries. Face-to-face interviews were conducted during November and December 2005. Usual weekday sitting time was assessed using the International Physical Activity Questionnaire (short-version). Sitting time was compared by country, age, gender, years of education, general health status, usual activity and physical activity. Multivariable-adjusted analyses assessed the odds of belonging to the highest sitting quartile.

Results

Data were available for 27,637 adults aged 15–98 years. Overall, mean reported weekday sitting time was 309 min/day (SD 184 min/day). There was a broad geographical pattern and some of the lowest amounts of daily sitting were reported in southern (Malta and Portugal means 194–236 min/day) and eastern (Romania and Hungary means 191–276 min/day) European countries; and some of the highest amounts of daily sitting were reported in northern European countries (Germany, Benelux and Scandinavian countries; means 407–335 min/day). Multivariable-adjusted analyses showed adults with low physical activity levels (OR = 5.10, CI95 = 4.60-5.66), those with high sitting in their main daily activity (OR = 2.99, CI95 = 2.74-3.25), those with a bad/very bad general health state (OR = 1.87, CI95 = 1.63-2.15) and higher education levels (OR = 1.48, CI95 = 1.38-1.59) were more likely to be in the highest quartile of daily sitting time. Adults within Greece (OR = 2.91, CI95 = 2.51-3.36) and Netherlands (OR = 2.56, CI95 = 2.22-2.94) were most likely to be in the highest quartile. High-sit/low-active participants comprised 10.1% of the sample. Adults self-reporting bad/very bad general health state (OR = 4.74, CI95 = 3.97-5.65), those within high sitting in their main daily activities (OR = 2.87, CI95 = 2.52-3.26) and adults aged ≥65 years (OR = 1.53, CI95 = 1.19-1.96) and were more likely to be in the high-sit/low-active group.

Conclusions

Weekday sitting time and its demographic correlates varied considerably across European countries, with adults in north-western European countries sitting the most. Sitting is prevalent across Europe and merits attention by preventive interventions.
Participatory study of medicinal plants used in the control of gastrointestinal parasites in donkeys in Eastern Shewa and Arsi zones of Oromia region, Ethiopia
Authors: Scantlebury Claire E , Peachey Laura , Hodgkinson Jane , Matthews Jacqui B , Trawford Andrew , Mulugeta Getachew , Tefera Gebre , Pinchbeck Gina L
Published: September 2013

Background

Gastrointestinal nematode infections constitute a threat to the health and welfare of donkeys worldwide. Their primary means of control is via anthelmintic treatments; however, use of these drugs has constraints in developing countries, including cost, limited availability, access to cheaper generic forms of variable quality and potential anthelmintic resistance. As an alternative, bioactive plants have been proposed as an option to treat and control gastrointestinal helminths in donkeys. This study aimed to use participatory methodology to explore donkey owner knowledge, attitudes and beliefs relating to the use of plant-based treatments for gastrointestinal parasites of donkeys in Ethiopia.

Results

In focus groups, 22/29 groups stated they knew of plants used for the treatment of gastrointestinal parasites in donkeys. All groups volunteered plants that were used in cattle and/or small ruminants. In total, 21 plants were named by participants. ‘Koso’ (Hagenia abyssinica) ‘Grawa’ (Vernonia amygdalina) and a mixed roots and leaves preparation were the most frequently named plant preparations. ‘Enkoko’(Embelia shimperi) and ‘a mixture of roots and leaves’ were ranked highly for effectiveness in donkeys. However, ‘Grawa’ and ‘Koso’ were the highest ranked when taking into account both the rank position and the number of groups ranking the plant.

Thematic analysis of participants’ current attitudes and beliefs surrounding traditional plant-based remedies for gastrointestinal parasites revealed that anthelmintics obtained from clinics were generally favoured due to their ease of administration and perceived higher effectiveness. There was doubt surrounding the effectiveness of some plant-based treatments, but there were also perceived advantages including their low cost, ease of cultivation and availability. However, plant-based treatments were considered a “past trend”and people favoured “modern”medicine, particularly among the younger generation.

Conclusions

There was extensive knowledge of plant-based treatments for gastrointestinal parasites in livestock in Ethiopia. In donkeys, Koso (Hagenia abyssinica), Grawa (Vernonia amygdalina), Enkoko (Embelia shimperi) and ‘mixed roots and leaves’ were the most frequently named and/or highest ranked plants with reported efficacy against gastrointestinal parasites. Furtherin vitroandin vivoinvestigation of these plants is now required to determine viable alternatives for the treatment and control of gastrointestinal parasites in Ethiopia.
Unfolded protein response in Gaucher disease: from human to Drosophila
Authors: Maor Gali , Rencus-Lazar Sigal , Filocamo Mirella , Steller Hermann , Segal Daniel , Horowitz Mia
Published: September 2013

Background

In Gaucher disease (GD), resulting from mutations in the GBA gene, mutant β-glucocerebrosidase (GCase) molecules are recognized as misfolded in the endoplasmic reticulum (ER). They are retrotranslocated to the cytoplasm, where they are ubiquitinated and undergo proteasomal degradation in a process known as the ER Associated Degradation (ERAD). We have shown in the past that the degree of ERAD of mutant GCase correlates with GD severity.

Persistent presence of mutant, misfolded protein molecules in the ER leads to ER stress and evokes the unfolded protein response (UPR).

Methods

We investigated the presence of UPR in several GD models, using molecular and behavioral assays.

Results

Our results show the existence of UPR in skin fibroblasts from GD patients and carriers of GD mutations. We could recapitulate UPR in two different Drosophila models for carriers of GD mutations: flies heterozygous for the endogenous mutant GBA orthologs and flies expressing the human N370S or L444P mutant GCase variants. We encountered early death in both fly models, indicating the deleterious effect of mutant GCase during development. The double heterozygous flies, and the transgenic flies, expressing mutant GCase in dopaminergic/serotonergic cells developed locomotion deficit.

Conclusion

Our results strongly suggest that mutant GCase induces the UPR in GD patients as well as in carriers of GD mutations and leads to development of locomotion deficit in flies heterozygous for GD mutations.
Optimising parameters for the differentiation of SH-SY5Y cells to study cell adhesion and cell migration
Authors: Dwane Susan , Durack Edel , Kiely Patrick A
Published: September 2013

Background

Cell migration is a fundamental biological process and has an important role in the developing brain by regulating a highly specific pattern of connections between nerve cells. Cell migration is required for axonal guidance and neurite outgrowth and involves a series of highly co-ordinated and overlapping signalling pathways. The non-receptor tyrosine kinase, Focal Adhesion Kinase (FAK) has an essential role in development and is the most highly expressed kinase in the developing CNS. FAK activity is essential for neuronal cell adhesion and migration.

Results

The objective of this study was to optimise a protocol for the differentiation of the neuroblastoma cell line, SH-SY5Y. We determined the optimal extracellular matrix proteins and growth factor combinations required for the optimal differentiation of SH-SY5Y cells into neuronal-like cells and determined those conditions that induce the expression of FAK. It was confirmed that the cells were morphologically and biochemically differentiated when compared to undifferentiated cells. This is in direct contrast to commonly used differentiation methods that induce morphological differentiation but not biochemical differentiation.

Conclusions

We conclude that we have optimised a protocol for the differentiation of SH-SY5Y cells that results in a cell population that is both morphologically and biochemically distinct from undifferentiated SH-SY5Y cells and has a distinct adhesion and spreading pattern and display extensive neurite outgrowth. This protocol will provide a neuronal model system for studying FAK activity during cell adhesion and migration events.
Test-retest reliability of the ‘Reading the Mind in the Eyes’ test: a one-year follow-up study
Authors: Fernández-Abascal Enrique G , Cabello Rosario , Fernández-Berrocal Pablo , Baron-Cohen Simon
Published: September 2013

Background

The ‘Reading the Mind in the Eyes’ (Eyes) test is an advanced test of theory of mind. It is widely used to assess individual differences in social cognition and emotion recognition across different groups and cultures. The present study examined distributions of responses and scores on a Spanish version of the test in a non-clinical Spanish adult population, and assessed test-retest reliability over a 1-year interval.

Methods

A total of 358 undergraduates of both sexes, age 18 to 65 years, completed the Spanish version of the test twice over an interval of 1 year. The Bland-Altman method was used to calculate test-retest reliability.

Results

Distributions of responses and scores were optimal. Test-retest reliability for total score on the Eyes test was .63 (P <.01), based on the intraclass correlation coefficient. Test-retest reliability using the Bland-Altman method was fairly good.

Conclusions

This is the first study providing evidence that the Eyes test is reliable and stable over a 1-year period, in a non-clinical sample of adults.
Environmental impacts of farm land abandonment in high altitude/mountain regions: a systematic map of the evidence
Authors: Haddaway Neal R , Styles David , Pullin Andrew S
Published: September 2013

Background

Environmental impacts of farm land abandonment can be viewed as either an opportunity for ecological restoration to a state prior to agricultural establishment, or as the loss of an on-going process of land management and an associated threat to biodiversity. Whether land abandonment poses an ecological opportunity or threat depends upon the agricultural history and the presence of ecological systems that depend upon regular management for their existence. In Europe, many ecosystems have developed in the presence of agriculture and the loss of continued management resulting from land abandonment can have significant negative ecological impacts. Around 56 percent of the utilised agricultural area (UAA) of the EU is classified as ‘less-favourable areas’ and much of this is mountainous. The small-scale and extensively managed farmlands that are common in mountain areas are particularly vulnerable to marginalisation and abandonment. The work herein will form the first systematic synthesis of the evidence of impacts of farm land abandonment in mountain areas across the globe.

Methods

This review will take the form of two interrelated systematic maps, cataloguing the existing evidence across a wide range of variables such as setting, methodology, scale, measured outcomes etc. Mapping will be undertaken both at abstract-level at a coarse scale and at full text-level at a finer scale. Literature databases, organisational web sites, and search engines will be used to collate all of the available literature regarding the impacts of agricultural land abandonment. All studies investigating farmland abandonment in mountainous regions with an appropriate comparator and measuring an appropriate outcome will be included. Outcomes will be coded in a partly iterative process but will include; natural hazards (fire-/flood risk, land/mud slides), soil (fertility, erosion), water (chemistry, eutrophication, sediment load, hydrology), ecosystem functioning (biodiversity, abundance, invasive species presence), socio-economics (e.g. health, wellbeing, employment). The systematic map outputs will be in the form of searchable databases of relevant and obtainable (full text only) literature, coded by subject, methodology and study design, and internal validity.
TNF receptor-associated factor 5 gene confers genetic predisposition to acute anterior uveitis and pediatric uveitis
Authors: Xiang Qin , Chen Lu , Fang Jing , Hou Shengping , Wei Lin , Bai Lin , Liu Yunjia , Zhou Yan , Kijlstra Aize , Yang Peizeng
Published: September 2013

Introduction

TNF Receptor-Associated Factor 5 (TRAF5) has been shown to be associated with autoimmune disease. The current study sought to investigate the potential association of TRAF5 with acute anterior uveitis (AAU) and pediatric uveitis in Han Chinese.

Methods

Three TRAF5 SNPs were analyzed in 450 AAU patients with or without ankylosing spondylitis (AS), 458 pediatric uveitis patients, and 1,601 healthy controls by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) or TaqMan SNP Genotyping Assay. Numerous variables were evaluated, including age, sex distribution, and clinical and laboratory observations.

Results

Two SNPs (rs6540679, rs12569232) of TRAF5 were associated with pediatric uveitis, and rs12569232 also showed a relation with the presence of microvascular leakage. No significant associations were found when patients were subdivided according to their rheumatoid factor (RF) or anti-nuclear antibody (ANA) status or whether they had juvenile idiopathic arthritis (JIA). Rs12569232 predisposed to AAU and its subgroups (with ankylosing spondylitis (AS) or HLA-B27 positive). No association was found between rs10863888 and either pediatric uveitis or AAU.

Conclusion

This study revealed that TRAF5 is involved in the development of AAU and pediatric uveitis. Further stratified analysis according to the clinical and laboratory observations suggested that rs12569232/TRAF5 may play a role in the development of retinal vasculitis.
Metabolic interaction between cancer cells and stromal cells according to breast cancer molecular subtype
Authors: Choi Junjeong , Kim Do Hee , Jung Woo Hee , Koo Ja Seung
Published: September 2013

Introduction

The aim of this study was to investigate the differential expression of markers related to metabolic, mitochondrial and autophagy status in different molecular subtypes of breast cancer.

Methods

Using tissue microarray sections generated from 740 cases of breast cancer, we performed immunohistochemical staining for Glut-1, CAIX, MCT4, ATP synthase, glutaminase, BNIP3, Beclin-1, LC3A, LC3B and p62. Based on the immunohistochemical expression of estrogen receptor (ER), progesterone (PR), HER2, and Ki-67 labeling index, the cases were classified into luminal A, luminal B, HER2 and triple-negative breast cancer (TNBC). We further classified metabolic phenotypes of tumors according to glycolytic status by assessing Glut-1 and CAIX expression as follows: Warburg type: tumor (glycolysis type), stroma (nonglycolysis type); reverse Warburg type: tumor (nonglycolysis type), stroma (glycolysis type); mixed type: tumor (glycolysis type), stroma (glycolysis type); and null type: tumor (nonglycolysis type), stroma (nonglycolysis type).

Results

Expression of Glut-1, MCT4 and LC3A was highest in TNBC and lowest in luminal A type (P < 0.001). Tumors were classified into 298 Warburg type (40.3%), 54 reverse Warburg type (7.3%), 62 mixed type (8.4%) and 326 null type (44.0%). The mixed type had a higher histologic grade, ER negativity, PR negativity and Ki-67 index, whereas the null type showed lower histologic grade, ER positivity, PR positivity and Ki-67 index (P < 0.001). TNBC constituted the major portion of Warburg and mixed types, and luminal A consisted mainly of reverse Warburg and null types (P < 0.001).

Conclusion

Breast cancer is heterogeneous in its metabolic status, and therefore it can be classified into various metabolic phenotypes. Specifically, the Warburg and mixed types had strong associations with TNBC, whereas reverse the Warburg and null types had associations with the luminal type, suggesting a correlation between metabolic phenotype and the biology of breast cancer.
A mutual activation loop between breast cancer cells and myeloid-derived suppressor cells facilitates spontaneous metastasis through IL-6 trans-signaling in a murine model
Authors: Oh Keunhee , Lee Ok-Young , Shon Suh Youn , Nam Onyou , Ryu Po Mee , Seo Myung Won , Lee Dong-Sup
Published: September 2013

Introduction

Tumor cell interactions with the microenvironment, especially those of bone-marrow-derived myeloid cells, are important in various aspects of tumor metastasis. Myeloid-derived suppressor cells (MDSCs) have been suggested to constitute tumor-favoring microenvironments. In this study, we elucidated a novel mechanism by which the MDSCs can mediate spontaneous distant metastasis of breast cancer cells.

Methods

Murine breast cancer cells, 4T1 and EMT6, were orthotopically grafted into the mammary fat pads of syngeneic BALB/c mice. CD11b+Gr-1+ MDSCs in the spleen, liver, lung and primary tumor mass were analyzed. To evaluate the role of MDSCs in the distant metastasis, MDSCs were depleted or reconstituted in tumor-bearing mice. To evaluate whether MDSCs in the metastasizing tumor microenvironment affect breast cancer cell behavior, MDSCs and cancer cells were co-cultivated. To investigate the role of MDSCs in in vivo metastasis, we blocked the interactions between MDSCs and cancer cells.

Results

Using a murine breast cancer cell model, we showed that murine breast cancer cells with high IL-6 expression recruited more MDSCs and that the metastasizing capacity of cancer cells paralleled MDSC recruitment in tumor-bearing mice. Metastasizing, but not non-metastasizing, tumor-derived factors induced MDSCs to increase IL-6 production and full activation of recruited MDSCs occurred in the primary tumor site and metastatic organ in the vicinity of metastasizing cancer cells, but not in lymphoid organs. In addition, tumor-expanded MDSCs expressed Adam-family proteases, which facilitated shedding of IL-6 receptor, thereby contributing to breast cancer cell invasiveness and distant metastasis through IL-6 trans-signaling. The critical role of IL-6 trans-signaling was confirmed in both the afferent and efferent pathways of metastasis.

Conclusion

In this study, we showed that metastasizing cancer cells induced higher MDSCs infiltration and prompted them to secret exaggerated IL-6 as well as soluble IL-6Rα, which, in turn, triggered a persistent increase of pSTAT3 in tumor cells. This potential tumor-MDSC axis involving IL-6 trans-signaling directly affected breast cancer cell aggressiveness, leading to spontaneous metastasis.
Critical illness induces nutrient-independent adipogenesis and accumulation of alternatively activated tissue macrophages
Authors: Marques Mirna Bastos , Perre Sarah Vander , Aertgeerts Annelies , Derde Sarah , Güiza Fabian , Casaer Michael P , Hermans Greet , Van den Berghe Greet , Langouche Lies
Published: September 2013

Introduction

We previously reported that in artificially-fed critically ill patients, adipose tissue reveals an increase in small adipocytes and accumulation of M2-macrophages. We hypothesized that nutrient-independent factors of critical illness explain these findings, and that the M2-macrophage accumulation may not be limited to adipose tissue.

Methods

In a long-term cecal ligation and puncture (CLP) mouse model of sepsis, we compared the effect of parenteral nutrition (CLP-fed, n = 13) with nutrient restriction (CLP-restricted, n = 11) on body composition, adipocyte size and macrophage accumulation in adipose tissue, liver and lungs. Fed healthy mice (n = 11) were studied as controls. In a human study, in vivo adipose tissue biopsies were studied from ICU patients (n = 40) enrolled in a randomized control trial which compared early initiation of parenteral nutrition (PN) versus tolerating nutrient restriction during the first week of ICU stay. Adipose tissue morphology was compared with healthy human controls (n = 13).

Results

Irrespective of nutritional intake, critically ill mice lost weight, fat and fat-free mass. Adipocyte number, proliferation marker Proliferating Cell Nuclear Antigen (PCNA) and adipogenic markers PPARγ and CCAAT/enhancer binding protein-β (C/EBPβ) increased with illness, irrespective of nutritional intake. M2-macrophage accumulation was observed in adipose tissue, liver and lungs of critically ill mice. Macrophage M2-markers correlated with CCL2 expression. In adipose tissue biopsies of critically ill patients, increased adipogenic markers and M2 macrophage accumulation were present irrespective of nutritional intake.

Conclusions

Adipogenesis and accumulation of tissue M2-macrophages are hallmarks of prolonged critical illness, irrespective of nutritional management. During critical illness, M2-macrophages accumulate not only in adipose tissue, but also in the liver and lungs.