bluuebunny/crossref_metadata_2025 huggingface dataset:
The effects of aerobic exercise on body composition in overweight and obese patients with gout: a randomized, open-labeled, controlled trial
Authors: Jia Ertao , Zhu Haiqiong , Geng Hongling , Liu Ruilin , Wo Xueqian , Zeng Yaochi , Ma Wukai , Yao Xueming , Zhan Zhiying , Zhang Jianyong
Published: September 2022

Background

Overweight and obesity are typical risk factors for the increased prevalence and incidence of gout. The existing guidelines unequivocally indicated that exercise is highly advantageous for patients with gout. Nevertheless, there is still a lack of specific guidance and clinical evidence. The effects of exercise on improving gout, and the optimal frequency, timing, and types of exercise have not been fully clarified. The present trial aims to determine the effects of a specific aerobic exercise program on body composition in overweight and obese patients with gout.

Methods

In this randomized, open-labeled, controlled trial, a total of 60 overweight and obese patients with gout [body mass index (BMI) ≥ 24 kg/m2; age,18–55 years old] are equally randomized (1:1) into two groups (n = 30): moderate-intensity aerobic exercise group (MIAEG), heart rate reserve (HRR) = [(HRmax-HRrest) × 60% intensity] + HRrest, and control group (CG). The moderate-intensity aerobic exercise training program will be conducted for 30–40 min/session and 3 days/week for 12 weeks. Participants in the CG will be asked to avoid making changes in their exercise habits. There will be no limitation in the type of exercise. The primary outcome is the number of patients whose body fat is reduced after 12 weeks. The secondary outcomes include the changes in BMI, waist-to-hip ratio (WHR), insulin resistance index (IRI), serum uric acid (sUA), serum creatinine (SCr), estimated glomerular filtration rate (eGFR), triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), hepatic steatosis, and adverse effects after 12 weeks. One-way analysis of variance (ANOVA) will be used to compare the mean values of normally distributed variables between MIAEG and GC.

Discussion

The effect and optimal frequency of exercise for improving the status of overweight and obese patients with gout have not yet been determined. We design a 12-week randomized controlled trial and evaluate the effects of individualized aerobic exercise program on patients with gout. The results may assist such patients with a personalized scientific exercise program based on the disease status and motor abilities, so that patients are prone to exercise under the condition of low risk and achieve the greatest benefits.

Trial registration

ChiCTR2200062153. Registered on July 25, 2022, with ChiCTR. http://www.chictr.org.cn/
Potential benefits of a virtual, home-based combined exercise and mindfulness training program for HSC transplant survivors: a single-arm pilot study
Authors: Ma David D. F. , Fennessy Kate , Kliman David
Published: September 2022

Purpose

Impaired quality of life (QOL) including reduced physical fitness is a recognized late effect of hemopoietic cell transplantation (HCT). Guided exercise and mindfulness-based stress management (MBSM) programs have shown promise, mainly in the inpatient setting. We aimed to examine the feasibility of a virtual, home-based, combined exercise and MBSM program.

Methods

Patients attending post-HCT clinic were invited to participate in this single-arm pre-post study. Eligibility criteria included age 18–75 years, > 6 months post allogeneic HCT. Consented participants attended an in-person session, followed by weekly exercise and MBSM training for 6 weeks via videoconferencing. Assessments were performed pre-training, and at 3-, 6- and 12-months and compared using a linear mixed effects model.

Results

21 of 24 patients consenting to the study completed the program (median age 56 years [IQR 46–62], median time post-HCT 37 months [IQR 26–46]). Six-minute walk test scores were significantly higher at 3 (mean difference 79.6, 95%CI 28–131, ES 0.55) and 12 months (mean difference 48.4, 95%CI 13–84, ES 0.33) compared to baseline. Sit-to-stand test was significantly higher at 3 (mean difference 4.4, 95%CI 1.4–7.4, ES 0.68) and 12 months (mean difference 3.9, 95%CI 0.24–7.6, ES 0.61). Dominant hand grip was significantly stronger at 3 (mean difference 0.16, 95%CI 0.04–0.28, ES 0.45), and 12 months (mean difference 0.21, 95%CI 0.08–0.24, ES 0.62). Significantly higher FACT-BMT total (mean difference 6.9, 95%CI 1.5–12.4, ES 0.49) and FACT-G scores (mean difference 5.2, 95%CI 1.4–9.1, ES 0.48) were found at 3 months. Over 80% of participants rated the virtual combined modal program highly and no adverse events were reported.

Conclusion

A 6-week virtual, home-based exercise and MBSM program was an acceptable, and potentially effective intervention for sustained improvement of some physical capacity and QOL outcomes in HCT survivors. Virtual-based healthcare service is highly relevant particularly during pandemics. To our knowledge, this study has the longest follow-up observation period for Internet based combined modality training program reported to date and warrants additional investigation.

Trial Registration Research protocol approved by St Vincent’s Hospital Ethics Committee (HREC 12/SVH/175), approved 27/09/2012, trial commenced 24/05/13 and the first participant 07/06/13. Retrospectively registered with ANZCTR (ACTRN12613001054707) 23/09/2013.
Hungarian male water polo players’ body composition can predict specific playing positions and highlight different nutritional needs for optimal sports performance
Authors: Fritz Péter , Fritz Réka , Mayer Lívia , Németh Boglárka , Ressinka Judit , Ács Pongrác , Oláh Csilla
Published: September 2022

Background

Water polo is unique among aquatic—and generally other—sports as it includes cyclic elements typical in swimming and acyclic elements occurring mainly in ball games. Moreover, water polo demands high level of technical and tactical skills. Players need an optimal nutritional and physical condition to achieve high athletic performance, which is to a great extend influenced by nutritional habits. We aim to highlight possible shortfalls in players’ nutritional intake in relation to positions played within the team.

Methods

In the present study, we determined the anthropometric and body composition characteristics, dietary habits and laboratory parameters of elite adult male water polo players (n = 19) before the start of the championship and at the end of the regular season, which meant a 4-month intervention period. Analyses of body composition characteristics and nutritional habits were performed using bioimpedance analyzer InBody 770 and a 3-day nutrition diary, respectively. Paired-sample t-test were used to determine the differences between the variables measured before and after the championship. Correlations between the anthropometric and body composition characteristics and different serum parameters were analyzed using linear correlation calculation. K-mean cluster analysis was performed using the anthropometric and body composition characteristics of the athletes.

Results

Based on anthropometric and body composition characteristics, players can be divided into two significantly different clusters that shows an association with specific playing positions. Cluster I included goalkeepers and wing players, while defenders, centers, and shooters belonged to Cluster II. We observed significant differences in the physical composition and slight but not significant differences in nutritional habits of the clusters. Cluster I players were 5 cm shorter on average, while their mean body weight, skeletal muscle mass and body fat mass data were lower by 19 kg, 7 kg, and 7 kg, respectively. We studied the correlation between initial anthropometric and body composition parameters and the changes in laboratory parameters before and after the regular season. As a result, we detected numerous significant differences between the two clusters, such as the changes in glucose and magnesium levels, which showed a strong correlation with several body composition parameters in cluster II, but did not in cluster I.

Conclusions

Cluster differences between anthropometric and body compositional characteristics, and the changes in laboratory parameters can help to develop position-specific training and nutritional recommendations in the future. Therefore, the results may be applicable in sport sciences for elite athletes and sports coaches.
Dried blood spot is the feasible matrix for detection of some but not all hepatitis B virus markers of infection
Authors: Kikuchi Minami , Lindstrom Patrick , Tejada-Strop Alexandra , Mixson-Hayden Tonya , Kamili Saleem , Sawabe Motoji
Published: September 2022

Objective

Use of dried blood spots (DBS) for detection of hepatitis B virus (HBV) markers of infection has the potential to facilitate diagnosis of HBV infection especially in resource-limited countries. The aim of this study was to evaluate the feasibility of DBS for detection of various markers of HBV infections.

Results

Fifty-four DBS samples were engineered from well-characterized plasma samples. All DBS samples were tested for HBsAg, total anti-HBc and HBV DNA, 20 of 54 samples were also tested for HBeAg using commercially available assays. HBsAg was detected in 24 of 25 (96%), HBV DNA in 22 of 25 (88%), total anti-HBc in all 9 (100%), and HBeAg in all 7 (100%) DBS samples. The average difference in HBV DNA levels between DBS eluates and corresponding plasma samples was 2.7 log10 IU/mL. Fifteen DBS eluates positive for HBV DNA were sequenced and all of them belonged to HBV genotype A. Thirteen samples which were negative for all HBV markers showed HBeAg false positivity. Therefore, DBS is a reliable sample matrix for detection of HBsAg, total anti-HBc and HBV DNA, but not HBeAg. Further feasibility studies of DBS for diagnostic purposes and epidemiologic studies are warranted.
Expression of osteopontin-5 splice variant in the mouse primary and metastatic breast cancer cells
Authors: Kamalabadi-Farahani Mohammad , Atashi Amir , Jabbarpour Zahra , Aghayan Seyed Sajjad
Published: September 2022

Objective

Osteopontin (OPN) is a well-known glycoprotein involved in numerous pathobiological processes, including cancer. Despite having five splice variants for osteopontin in mice, the main focus of most studies has been on total OPN (tOPN). There are some studies on other splice variants, but the expression of osteopontin-5 (OPN5) has not been addressed in mouse cancer cells. Therefore, this study sought to evaluate OPN5 expression in mouse breast cancer cells.

Results

The expression of OPN5 in primary and metastatic breast cancer cells of mice was confirmed in our study. These findings provided important insights regarding the OPN alternative splicing in mice for the first time. It is concluded that, like other OPN-SVs, OPN5 probably plays an essential role in tumor progression, which requires further investigation in different tumor models.
Efficacy and safety of dutasteride with tadalafil add-on therapy in patients with lower urinary tract symptoms secondary to benign prostatic hyperplasia
Authors: Gotoh Daisuke , Torimoto Kazumasa , Morizawa Yosuke , Hori Shunta , Nakai Yasushi , Miyake Makito , Fujimoto Kiyohide
Published: September 2022

Objective

To evaluate the efficacy and safety of add-on therapy with the phosphodiesterase type 5 inhibitor tadalafil in Japanese men with lower urinary tract symptoms (LUTS) due to benign prostatic hyperplasia (BPH) treated with dutasteride.

Results

Twenty-four patients were enrolled. The participants had a median age of 71.0 (64.8–73.0) years and a median prostate volume of 37.3 (29.7–41.8) mL as measured using transabdominal sonography. The efficacy indicators, such as International Prostate Symptom Score (IPSS), quality of life (QOL) score, night-time urinary frequency, and night-time maximum voided volume, improved significantly at 4 weeks, and the effects lasted until 24 weeks (IPSS: 9.5 vs. 17.0, QOL: 2.0 vs. 4.0, nocturia: 2.0 vs. 2.0, night-time maximum voided volume: 290.0 vs. 240.0 mL). Overactive bladder symptom score (OABSS) and sexual health inventory for men (SHIM) significantly improved at 12 weeks, and the effects lasted until 24 weeks (OABSS: 3.0 vs. 5.0, SHIM: 11.0 vs. 7.5). However, maximum urine flow and residual urine volume showed no improvement at any point. Adverse events occurred in two cases. Taken together, add-on therapy with tadalafil was effective for patients with LUTS/BPH resistant to dutasteride monotherapy. In addition, this therapy was not associated with severe adverse events.
Interventions and methods to prepare, educate or familiarise children and young people for radiological procedures: a scoping review
Authors: Bray Lucy , Booth Lisa , Gray Victoria , Maden Michelle , Thompson Jill , Saron Holly
Published: September 2022

Children attending hospital for radiological procedures can experience uncertainty, anxiety and distress; this can result in sub-optimal experiences for children, poor scan quality and the need for radiological procedures to be rescheduled or sedation to be used. The preparation and education of children before clinical procedures has been shown to have a positive influence on procedural outcomes. This scoping review aimed to locate and examine the evidence relating to non-invasive interventions and methods to prepare, educate and familiarise children for radiological procedures within a healthcare setting. A comprehensive search strategy identified 36 articles. A narrative synthesis approach was adopted to make sense of the key findings. Studies investigated a range of radiological procedures (MRI, plain radiographs, CT, fluoroscopy and Micturating cystourethrogram) using a wide range of interventions (smartphone applications, storybooks, videos, mock scanners) which varied by method, mode of delivery and target audience. The outcomes used to evaluate the value and impact of the interventions are wide, varied and inconsistently applied making it difficult to judge which interventions offer the optimal impact on scan quality, scan completion and children’s experiences. This review highlights that there is a need to further understand which specific elements of the non-invasive interventions ‘work best’ for children. There is a need for consistency on the outcomes measured and for these measures to include child-centred outcomes alongside scan quality and length of radiological procedure.
Multiparametric MRI with MR elastography findings in patients with sinusoidal obstruction syndrome after oxaliplatin-based chemotherapy
Authors: Poker Ahmet , Karcaaltıncaba Musturay , Ozmen Mustafa N. , Karaosmanoğlu Ali D. , Erdemir Ahmet G. , Ocal Osman , Akata Deniz , Idilman Ilkay S.
Published: September 2022

Objective

To evaluate the magnetic resonance elastography (MRE)-derived liver stiffness measurement (LSM), T1 and T2 relaxation times, and hepatobiliary phase images in patients, who developed sinusoidal obstruction syndrome (SOS) after oxaliplatin-based chemotherapy.

Methods

Thirty-four patients (M/F:22/12) who underwent liver MRI-MRE and received oxaliplatin for colorectal, gastric, and pancreas cancer were included in the study. SOS was diagnosed by Gd-EOB-DTPA-enhanced MRI in 18 patients. MRE-LSM and T1–T2 maps were evaluated. Patients with SOS were grouped according to the amount of reticular hypointensity on the hepatobiliary phase images.

Results

The mean MRE-LSM in the patients with SOS was 3.14 ± 0.45 kPa, and the control group was 2.6 ± 0.5 kPa (p = 0.01). The mean-corrected T1 (cT1) relaxation time was 1181 ± 151 ms in the SOS group and 1032 ± 129 ms in the control group (p = 0.005). The mean T2 relaxation time was 50.29 ± 3.6 ms in the SOS group and 44 ± 3.9 ms in the control group (p = 0.01). Parenchymal stiffness values were 2.8 ± 0.22 kPa, 3 ± 0.33 kPa, and 3.65 ± 0.28 kPa in patients with mild, moderate, and advanced SOS findings, respectively (p = 0.002). Although cT1 and T2 relaxation times increased with increasing SOS severity, no statistical significance was found.

Conclusions

We observed increased MRE-LSM in patients with SOS after chemotherapy compared to control group. T1 and T2 relaxation times were also useful in diagnosing SOS but were found inadequate in determining SOS severity. MRE is effective in diagnosing SOS and determining SOS severity in patients who cannot receive contrast agents, and it may be useful in the follow-up evaluation of these patients.
Rejuvenated endothelial progenitor cells through overexpression of cellular prion protein effectively salvaged the critical limb ischemia in rats with preexisting chronic kidney disease
Authors: Yeh Jui-Po , Sung Pei‐Hsun , Chiang John Y. , Huang Chi-Ruei , Chen Yi-Ling , Lai Jui-Pin , Sheu Jiunn-Jye
Published: September 2022

Background

This study tested the hypothesis that overexpression of cellular prion protein in endothelial progenitor cells (PrPcOE-EPCs), defined as “rejuvenated EPCs,” was superior to EPCs for salvaging the critical limb ischemia (CLI) induced after 28-day chronic kidney disease (CKD) induction in rat.

Methods and Results

Cell viability and flow cytometric analyses of early/late apoptosis/total-intracellular ROS/cell cycle (sub-G1, G2/M phase) were significantly higher in EPCs + H2O2 than in EPCs that were significantly reversed in PrPcOE-EPCs + H2O2 (all p < 0.001). The protein expressions of inflammation (IL-1ß/IL-6/MMP-9/p-NF-κB) were significantly increased in EPC + TNF-α than in EPCs that were significantly reversed in PrPcOE-EPCs + TNF-α (all p < 0.001). Adult-male SD rats (n = 8/each group) were categorized into group 1 (sham-operated control), group 2 (CKD + CLI), group 3 [CKD + CLI + EPCs by intravenous (0.6 × 105)/intra-muscular (0.6 × 105) injections at 3 h after CLI induction], group 4 (CKD + CLI + PrPcOE-EPCs/dose-administration as group 3) and group 5 (CKD + CLI + siPrnp-EPCs/dose-administration as group 3). By day 14 after CLI induction, the ratio of ischemia to normal blood flow (INBF) in CLI area was highest in group 1/lowest in group 2/significantly higher in group 4 than in groups 3/5 and significantly higher in group 3 than in group 5 (all p < 0.0001). Histopathology demonstrated that the angiogenesis (number of small vessels/CD31 + cells) exhibited a similar trend, whereas the fibrosis/kidney injury score exhibited an opposite pattern of INBF among the groups (all p < 0.0001). The protein expressions of angiogenesis (SDF-1α/VEGF/CXCR4)/cell-stress signaling (p-PI3K/p-Akt/p-m-TOR) were significantly and progressively increased from groups 1–4 that were reversed in group 5 (all p < 0.0001). The protein expressions of fibrotic (p-Smad3/TGF-ß)/oxidative-stress (NOX-1/NOX-2/oxidized-protein)/apoptotic (mitochondrial-Bax/cleaved caspase3/cleaved PARP)/mitochondrial-damaged (cytosolic-cytochrome-C) biomarkers displayed an opposite pattern of INBF among the groups (all p < 0.0001).

Conclusion

PrPcOE-EPCs were superior to EPCs only therapy for salvaging the CLI.
Topical and intravenous administration of human umbilical cord mesenchymal stem cells in patients with diabetic foot ulcer and peripheral arterial disease: a phase I pilot study with a 3-year follow-up
Authors: Zhang Che , Huang Li , Wang Xiaofen , Zhou Xiaoya , Zhang Xiaoxian , Li Ling , Wu Jieying , Kou Meng , Cai Cheguo , Lian Qizhou , Zhou Xihui
Published: September 2022

Background

Diabetic foot ulcer (DFU) is a serious chronic complication of diabetes mellitus that contributes to 85% of nontraumatic lower extremity amputations in diabetic patients. Preliminary clinical benefits have been shown in treatments based on mesenchymal stem cells for patients with DFU or peripheral arterial disease (PAD). However, the long-term safety and benefits are unclear for patients with both DFU and PAD who are not amenable to surgical revascularization.

Methods

In this phase I pilot study, 14 patients with PAD and incurable DFU were enrolled to assess the safety and efficacy of human umbilical cord mesenchymal stem cell (hUC-MSC) administration based on conservative treatments. All patients received topical and intravenous administrations of hUC-MSCs at a dosage of 2 × 105cells/kg with an upper limit of 1 × 107cells for each dose. The adverse events during treatment and follow-up were documented for safety assessments. The therapeutic efficacy was assessed by ulcer healing status, recurrence rate, and 3-year amputation-free rate in the follow-up phase.

Results

The safety profiles were favorable. Only 2 cases of transient fever were observed within 3 days after transfusion and considered possibly related to hUC-MSC administration intravenously. Ulcer disclosure was achieved for more than 95% of the lesion area for all patients within 1.5 months after treatment. The symptoms of chronic limb ischaemia were alleviated along with a decrease in Wagner scores, Rutherford grades, and visual analogue scale scores. No direct evidence was observed to indicate the alleviation of the obstruction in the main vessels of target limbs based on computed tomography angiography. The duration of rehospitalization for DFU was 2.0 ± 0.6 years. All of the patients survived without amputation due to the recurrence of DFU within 3 years after treatments.

Conclusions

Based on the current pilot study, the preliminary clinical benefits of hUC-MSCs on DFU healing were shown, including good tolerance, a shortened healing time to 1.5 months and a favorable 3-year amputation-free survival rate. The clinical evidence in the current study suggested a further phase I/II study with a larger patient population and a more rigorous design to explore the efficacy and mechanism of hUC-MSCs on DFU healing.

Trial registration: The current study was registered retrospectively on 22 Jan 2022 with the Chinese Clinical Trial Registry (ChiCTR2200055885),http://www.chictr.org.cn/showproj.aspx?proj=135888

Graphical Abstract
Hsa_circ_0001485 promoted osteogenic differentiation by targeting BMPR2 to activate the TGFβ-BMP pathway
Authors: Chen Shan-Chuang , Jiang Tao , Liu Qi-Yu , Liu Zi-Tao , Su Yu-Fei , Su Hai-Tao
Published: September 2022

Background

Circular RNAs (circRNAs) are a new type of stable noncoding RNA and have been proven to play a crucial role in osteoporosis. This study explored the role and mechanism of hsa_circ_0001485 in osteogenic differentiation.

Methods

Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Gene Ontology (GO) enrichment analysis were performed according to the previous sequencing data in human bone marrow mesenchymal stem cells (BMSC) before and after the induction of osteogenic differentiation on the differentially expressed circRNAs, to screen out signaling pathways associated with osteogenic differentiation. The hFOB 1.19 cells were used to verify the function and mechanism of specific circRNAs in osteogenic differentiation. Additionally, small interfering fragments and overexpression plasmids were used to determine the role of specific circRNAs during osteogenic differentiation. Furthermore, pull-down experiments and mass spectrometry were performed to determine the proteins that bind to specific circRNAs.

Results

The KEGG and GO enrichment analyses showed that the TGFβ-BMP signaling pathway was related to the osteogenic differentiation process, and four circRNAs were associated with the pathway. The quantitative polymerase chain reaction analysis revealed that hsa_circ_0001485 expression was increased during the osteogenic differentiation process of BMSCs. Knockdown of hsa_circ_0001485 suppressed the activity of the alkaline phosphatase enzyme and the expression of RUNX2, osteopontin, and osteocalcin in the osteogenic hFOB 1.19 cells, whereas overexpression of hsa_circ_0001485 promoted their expression. Additionally, we found that hsa_circ_0001485 and BMPR2 targeted binding to activate the TGFβ-BMP signaling pathway and promoted osteogenic differentiation through mass spectrometry analysis.

Conclusion

This study demonstrates that hsa_circ_0001485 is highly expressed in the osteogenic hFOB 1.19 cells, which activate the TGFβ-BMP pathway through targeted binding of BMPR2, and plays a positive role in regulating osteogenic differentiation.
Exopolysaccharide of Enterococcus faecium L15 promotes the osteogenic differentiation of human dental pulp stem cells via p38 MAPK pathway
Authors: Kim Hyewon , Oh Naeun , Kwon Mijin , Kwon Oh-Hee , Ku Seockmo , Seo Jeongmin , Roh Sangho
Published: September 2022

Background

Bone has important functions in the body. Several researchers have reported that the polysaccharides and lipopolysaccharide derived from microbes can promote osteogenic differentiation of stem cells.Enterococcus faecium,a lactic acid bacterium (LAB), produces several bioactive metabolites and has been widely applied in the food and nutraceutical industries. The exopolysaccharide (EPS) from LAB has also been extensively examined for its postbiotic effects and for its in vivo and in vitro functionalities. However, studies on promoting bone differentiation using polysaccharides from LAB are lacking. Therefore, the purpose of this study was to investigate the effect ofE. faeciumL15 extract and EPS on osteogenic differentiation of human dental pulp stem cells (hDPSCs) and to identify the underlying mechanisms.

Methods

hDPSCs were obtained from dental pulp tissue, and L15 extract and EPS were isolated from L15. Gene and protein expression of the osteogenic differentiation markers were analyzed with qPCR and western blotting and the possible signaling pathways were also investigated using western blotting. Osteogenic differentiation potential was examined by alkaline phosphatase (ALP) staining and alizarin red s (ARS) staining. In addition, osteogenic differentiation potential of L15 EPS was explored in ex vivo culture of neonate murine calvaria.

Results

The calcium deposition and ALP activity were enhanced by addition of L15 extract or EPS. The expression levels ofRUNX2,ALP, andCOL1A1mRNA and the protein expression levels of RUNX2, ALP, and BMP4 were increased in hDPSCs treated with the L15 extract or EPS. The L15 EPS treatment enhanced phosphorylation of the p38 mitogen-activated protein kinase (MAPK). The L15 EPS-induced increases in RUNX2, ALP, and BMP4 expression were suppressed by the p38 MAPK inhibitor SB203580. The promoting effect of L15 EPS on osteogenic differentiation was not only seen in hDPSCs, but also in osteoblast precursors. ALP activity and the expression ofRUNX2,ALP, andCOL1A1increased in the L15 EPS-treated osteoblast precursors. In addition, L15 EPS increased bone thickness of neonate murine calvaria in ex vivo culture.

Conclusions

The stimulatory effect of L15 extract and EPS on osteogenic differentiation occurred through the p38 MAPK pathway, and L15 EPS enhanced new bone formation in neonate murine calvaria. These data suggest that L15 EPS has therapeutic potential applicable to bone regeneration.
Efficacy of stem cell therapy in animal models of intracerebral hemorrhage: an updated meta-analysis
Authors: Li Chenchen , Qin Haiyun , Zeng Liuwang , Hu Zhiping , Chen Chunli
Published: September 2022

Background

Multiple studies have reported that stem cell therapy has beneficial effects in animal models of intracerebral hemorrhage (ICH). However, this finding remains inconclusive. This study was performed to systematically determine the effect size of stem cell therapy in ICH animal models by pooling and analyzing data from newly published studies.

Methods

A literature search identified studies of stem cells in animal models of ICH. We searched mainstream databases from inception to November, 2021. And pooled effect size of stem cells was determined for diversified neurobehavioral scales and structural endpoints using random effects models.

Results

The median quality score of 62 included studies was 5.32. Our results revealed an overall positive effect of stem cell therapy. More specifically, the SMD was − 2.27 for mNSS, − 2.14 for rotarod test, − 2.06 for MLPT, − 1.33 for cylinder test, − 1.95 for corner turn test, − 1.42 for tissue loss, and − 1.86 for brain water content. For mNSS, classifying comparisons by quality score showed significant differences in estimates of effect size (p = 0.013), and high-quality comparisons showed a better outcome (SMD = − 2.57) compared with low-quality comparisons (SMD = − 1.59). Besides, different delivery routes also showed a significant difference in the estimates of effect size for mNSS (p = 0.002), and the intraperitoneal route showed the best outcome (SMD = − 4.63). For tissue loss, the autologous blood-induced ICH model showed a better outcome (SMD = − 1.84) compared with the collagenase-induced ICH model (SMD = − 0.94, p = 0.035). Additionally, stem cell therapy initiated within 8 h post-ICH showed the greatest efficacy on tissue loss reduction, followed by initiated with 24 h post-ICH. Finally, stem cells with different sources and types showed similar beneficial effects for mNSS as well as tissue loss.

Conclusions

Our results suggested that stem cell therapy had remarkable benefits on ICH animals on both the functional and structural outcomes in animal models of ICH, with very large effect size. These findings support the utility of further studies to translate stem cells in the treatment of ICH in humans. Moreover, the results should be interpreted in the light of the limitations in experimental design and the methodological quality of the studies included in the meta-analysis.
Optimal imaging time points considering accuracy and precision of Patlak linearization for 89Zr-immuno-PET: a simulation study
Authors: Wijngaarden Jessica E. , Huisman Marc C. , Pouw Johanna E. E. , Menke-van der Houven van Oordt C. Willemien , Jauw Yvonne W. S. , Boellaard Ronald
Published: September 2022

Purpose

Zirconium-89-immuno-positron emission tomography (89Zr-immuno-PET) has enabled visualization of zirconium-89 labelled monoclonal antibody (89Zr-mAb) uptake in organs and tumors in vivo. Patlak linearization of 89Zr-immuno-PET quantification data allows for separation of reversible and irreversible uptake, by combining multiple blood samples and PET images at different days. As one can obtain only a limited number of blood samples and scans per patient, choosing the optimal time points is important. Tissue activity concentration curves were simulated to evaluate the effect of imaging time points on Patlak results, considering different time points, input functions, noise levels and levels of reversible and irreversible uptake.

Methods

Based on 89Zr-mAb input functions and reference values for reversible (VT) and irreversible (Ki) uptake from literature, multiple tissue activity curves were simulated. Three different 89Zr-mAb input functions, five time points between 24 and 192 h p.i., noise levels of 5, 10 and 15%, and three reference Ki and VT values were considered. Simulated Ki and VT were calculated (Patlak linearization) for a thousand repetitions. Accuracy and precision of Patlak linearization were evaluated by comparing simulated Ki and VT with reference values.

Results

Simulations showed that Ki is always underestimated. Inclusion of time point 24 h p.i. reduced bias and variability in VT, and slightly reduced bias and variability in Ki, as compared to combinations of three later time points. After inclusion of 24 h p.i., minimal differences were found in bias and variability between different combinations of later imaging time points, despite different input functions, noise levels and reference values.

Conclusion

Inclusion of a blood sample and PET scan at 24 h p.i. improves accuracy and precision of Patlak results for 89Zr-immuno-PET; the exact timing of the two later time points is not critical.
Change in brain amyloid load and cognition in patients with amnestic mild cognitive impairment: a 3-year follow-up study
Authors: Rauhala Elina , Johansson Jarkko , Karrasch Mira , Eskola Olli , Tolvanen Tuula , Parkkola Riitta , Virtanen Kirsi A. , Rinne Juha O.
Published: September 2022

Background

Our aim was to investigate the discriminative value of 18F-Flutemetamol PET in longitudinal assessment of amyloid beta accumulation in amnestic mild cognitive impairment (aMCI) patients, in relation to longitudinal cognitive changes.

Methods

We investigated the change in 18F-Flutemetamol uptake and cognitive impairment in aMCI patients over time up to 3 years which enabled us to investigate possible association between changes in brain amyloid load and cognition over time. Thirty-four patients with aMCI (mean age 73.4 years, SD 6.6) were examined with 18F-Flutemetamol PET scan, brain MRI and cognitive tests at baseline and after 3-year follow-up or earlier if the patient had converted to Alzheimer´s disease (AD). 18F-Flutemetamol data were analyzed both with automated region-of-interest analysis and voxel-based statistical parametric mapping.

Results

18F-flutemetamol uptake increased during the follow-up, and the increase was significantly higher in patients who were amyloid positive at baseline as compared to the amyloid-negative ones. At follow-up, there was a significant association between 18F-Flutemetamol uptake and MMSE, logical memory I (immediate recall), logical memory II (delayed recall) and verbal fluency. An association was seen between the increase in 18F-Flutemetamol uptake and decline in MMSE and logical memory I scores.

Conclusions

In the early phase of aMCI, presence of amyloid pathology at baseline strongly predicted amyloid accumulation during follow-up, which was further paralleled by cognitive declines. Inversely, some of our patients remained amyloid negative also at the end of the study without significant change in 18F-Flutemetamol uptake or cognition. Future studies with longer follow-up are needed to distinguish whether the underlying pathophysiology of aMCI in such patients is other than AD.
Population history, genetic variation, and conservation status of European white elm (Ulmus laevis Pall.) in Poland
Authors: Litkowiec Monika , Chudzińska Magdalena , Pasławska Anna , Pałucka Małgorzata , Kozioł Czesław , Lewandowski Andrzej
Published: September 2022

Key message

The core populations of the European white elm (Ulmus laevis Pall.) located in Poland maintained slightly higher level of genetic diversity compared to the peripheral populations of this species.

Context

The most severe threat to elms is the loss of natural habitat under the pressures of agriculture and forestry as well as urbanization. The reductions in European white elm populations as well as populations of other elm species have also been caused by Dutch elm disease (DED). Previous studies have indicated a low level of genetic variation in Ulmus leavis Pall. However, in Poland, the genetic resources and demographic history of U. laevis populations remain poorly documented.

Aims

The genetic resources of U. laevis in Poland were identified and characterized. Additionally, tests were performed to identify potential bottleneck signatures and effective population sizes of the examined populations.

Methods

Polymorphism was analyzed using a set of six nuclear microsatellite markers (nSSRs) for 1672 individuals from 41 populations throughout the species range in Poland.

Results

(1) A moderate level of genetic variation was found. (2) A low genetic differentiation and lack of population structuring were identified. (3) Evidence of reduction in population size was found as a consequence of severe, past bottlenecks.

Conclusion

The loss of genetic diversity of U. laevis probably occurred in their refugia or shortly after the postglacial recolonization. This loss may have been affected by past DED pandemics similar to those seen at present.
Asynchronous dissipative control for networked time-delay Markov jump systems with event-triggered scheme and packet dropouts
Authors: Chen Huiying , Liu Renwei , He Ping , Li Zuxin
Published: September 2022

This paper is concerned with asynchronous dissipative control for a class of networked time-delay Markov jump systems with event-triggered scheme and packet dropouts. To reduce communication consumption, an event-triggered transmission scheme is introduced. The phenomenon of packet dropout during the communication between the controller and the actuator is described by the Bernoulli model. The designed dissipative controller is asynchronous with the physical plant, which is described by a hidden Markov model. Based on a mode-dependent Lyapunov–Krasovskii function, a sufficient condition for the closed-loop control system to be stochastically stable and strictly $$(\mu ,\vartheta ,\upsilon ){-}\gamma$$

(
μ
,
ϑ
,
υ
)
-
γ

-dissipative is obtained. Furthermore, the design of dissipative controller is simplified by using matrix scaling and slack matrix techniques. Finally, a robotic arm system is used as an example to verify the effectiveness of our proposed design method.
Intelligent hyperspectral target detection for reliable IoV applications
Authors: Wang Zixu , Jin Lizuo , Yi Kaixiang
Published: September 2022

In recent years, hyperspectral imagery has played a significant role in IoV (Internet of Vehicles) vision areas such as target acquisition. Researchers are focusing on integrating detection sensors, detection computing units, and communication units into vehicles to expand the scope of target detection technology with hyperspectral imagery. As imaging spectroscopy technology gradually matures, the spectral resolution of captured hyperspectral images is increasing. At the same time, the volume of data is also increasing. As a result, the reliability of IoV applications is challenged. In this paper, an intelligent hyperspectral target detection method based on deep learning network is proposed. It is based on the residual network structure with the addition of an attention mechanism. The trained network model requires few computational resources and can provide the results in a short time. Our method improves the value of mAP50 by an average of 3.57% for all categories and by up to 5% for a single category on the public dataset.
A security method of hardware Trojan detection using path tracking algorithm
Authors: Huang Der-Chen , Hsiao Chun-Fang , Chang Tin-Wei , Chu Ying-Yi
Published: September 2022

Recently, the issue of malicious circuit alteration and attack draws more attention than ever before due to the globalization of IC design and manufacturing. Malicious circuits, also known as hardware Trojans, are found able to degrade the circuit performance or even leak confidential information, and accordingly it is definitely an issue of immediate concern to develop detection techniques against hardware Trojans. This paper presents a ring oscillator-based detection technique to improve the hardware Trojan detection performance. A circuit under test is divided into a great number of blocks, path assignment is optimized using a path tracking algorithm, and a high coverage is reached accordingly.
Systematic reviews are rarely used to contextualise new results—a systematic review and meta-analysis of meta-research studies
Authors: Draborg Eva , Andreasen Jane , Nørgaard Birgitte , Juhl Carsten Bogh , Yost Jennifer , Brunnhuber Klara , Robinson Karen A. , Lund Hans
Published: September 2022

Background

Results of new studies should be interpreted in the context of what is already known to compare results and build the state of the science. This systematic review and meta-analysis aimed to identify and synthesise results from meta-research studies examining if original studies within health use systematic reviews to place their results in the context of earlier, similar studies.

Methods

We searched MEDLINE (OVID), EMBASE (OVID), and the Cochrane Methodology Register for meta-research studies reporting the use of systematic reviews to place results of original clinical studies in the context of existing studies. The primary outcome was the percentage of original studies included in the meta-research studies using systematic reviews or meta-analyses placing new results in the context of existing studies. Two reviewers independently performed screening and data extraction. Data were synthesised using narrative synthesis and a random-effects meta-analysis was performed to estimate the mean proportion of original studies placing their results in the context of earlier studies. The protocol was registered in Open Science Framework.

Results

We included 15 meta-research studies, representing 1724 original studies. The mean percentage of original studies within these meta-research studies placing their results in the context of existing studies was 30.7% (95% CI [23.8%, 37.6%], I2=87.4%). Only one of the meta-research studies integrated results in a meta-analysis, while four integrated their results within a systematic review; the remaining cited or referred to a systematic review. The results of this systematic review are characterised by a high degree of heterogeneity and should be interpreted cautiously.

Conclusion

Our systematic review demonstrates a low rate of and great variability in using systematic reviews to place new results in the context of existing studies. On average, one third of the original studies contextualised their results. Improvement is still needed in researchers’ use of prior research systematically and transparently—also known as the use of an evidence-based research approach, to contribute to the accumulation of new evidence on which future studies should be based.

Systematic review registration

Open Science registration number https://osf.io/8gkzu/
Private anomaly detection of student health conditions based on wearable sensors in mobile cloud computing
Authors: Xie Yu , Zhang Kuilin , Kou Huaizhen , Mokarram Mohammad Jafar
Published: September 2022

With the continuous spread of COVID-19 virus, how to guarantee the healthy living of people especially the students who are of relative weak physique is becoming a key research issue of significant values. Specifically, precise recognition of the anomaly in student health conditions is beneficial to the quick discovery of potential patients. However, there are so many students in each school that the education managers cannot know about the health conditions of students in a real-time manner and accurately recognize the possible anomaly among students quickly. Fortunately, the quick development of mobile cloud computing technologies and wearable sensors has provided a promising way to monitor the real-time health conditions of students and find out the anomalies timely. However, two challenges are present in the above anomaly detection issue. First, the health data monitored by massive wearable sensors are often massive and updated frequently, which probably leads to high sensor-cloud transmission cost for anomaly detection. Second, the health data of students are often sensitive enough, which probably impedes the integration of health data in cloud environment even renders the health data-based anomaly detection infeasible. In view of these challenges, we propose a time-efficient and privacy-aware anomaly detection solution for students with wearable sensors in mobile cloud computing environment. At last, we validate the effectiveness and efficiency of our work via a set of simulated experiments.
Tourism cloud management system: the impact of smart tourism
Authors: Yin Fang , Yin Xiong , Zhou Jincheng , Zhang Xinli , Zhang Ruihua , Ibeke Ebuka , Iwendi Marvellous GodsPraise , Shah Mohammad
Published: September 2022

This study investigates the possibility of supporting tourists in a foreign land intelligently by using the Tourism Cloud Management System (TCMS) to enhance and better their tourism experience. Some technologies allow tourists to highlight popular tourist routes and circuits through the visualisation of data and sensor clustering approaches. With this, a tourist can access the shared data on a specific location to know the sites of famous local attractions, how other tourists feel about them, and how to participate in local festivities through a smart tourism model. This study surveyed the potential of smart tourism among tourists and how such technologies have developed over time while proposing a TCMS. Its goals were to make physical/paper tickets redundant via the introduction of a mobile app with eTickets that can be validated using camera and QR code technologies and to enhance the transport network using Bluetooth and GPS for real-time identification of tourists’ presence. The results show that a significant number of participants engage in tourist travels, hence the need for smart tourism and tourist management. It was concluded that smart tourism is very appealing to tourists and can improve the appeal of the destination if smart solutions are implemented. This study gives a first-hand review of the preference of tourists and the potential of smart tourism.
Sexual and reproductive health needs and practices of female sex workers in Papua New Guinea: findings from a biobehavioral survey Kauntim mi tu (‘Count me too’)
Authors: Weikum Damian , Kelly-Hanku Angela , Neo-Boli Ruthy , Aeno Herick , Badman Steven G. , Vallely Lisa M. , Willie Barne , Kupul Martha , Hou Parker , Amos Angelyn , Narokobi Rebecca , Pekon Simon , Coy Kelsey , Wapling Johanna , Gare Janet , Kaldor John M. , Vallely Andrew J. , Hakim Avi J. ,   on behalf of the Kauntim mi tu Study Team
Published: September 2022

Background

Little research has explored the sexual and reproductive health (SRH) experience of female sex workers (FSW), including girls aged < 18 years who are commercially sexually exploited (CSE), in Papua New Guinea (PNG). This paper describes the SRH history of FSW and CSE girls and factors associated with their use of moderately or highly effective contraceptive methods in three settings in PNG.

Methods

From 2016 to 2017, respondent-driven sampling (RDS) surveys were conducted among FSW and CSE girls in Port Moresby, Lae, and Mt. Hagen. FSW and CSE girls who were born female, aged ≥12 years, sold or exchanged vaginal sex in the past 6 months, spoke English or Tok Pisin, and had a valid RDS study coupon were eligible to participate. Interviews were conducted face-to-face and participants were offered rapid routine HIV and syphilis testing. Survey logistic regression procedures were used to identify factors associated with the use of moderately or highly effective contraceptive methods. Weighted data analysis was conducted.

Results

A total of 2901 FSW and CSE girls (Port Moresby, 673; Lae, 709; and Mt. Hagen, 709) were enrolled. The proportion using moderately or highly effective contraceptive methods was 37.7% in Port Moresby, 30.9% in Lae, and 26.5% in Mt. Hagen. After adjusting for covariates, factors significantly associated with the use of moderately or highly effective contraceptive methods in Port Moresby were being age 20–24, being married, being divorced or separated, having one or more dependent children, being away from home for more than 1 month in the last 6 months, and having tested HIV negative. No factors were significantly associated in Lae or Mt. Hagen. ANC attendance amongst FSW and CSE girls who gave birth in last 3 years was highest in Port Moresby at 91.2%. HIV testing was inconsistently and inadequately offered at ANC across the three cities.

Conclusions

Kauntim mi tu provides much-needed insight into the SRH experiences of FSW and CSE girls in PNG, where their use of moderately or highly effective contraceptive methods is low. We hope to shed light on the complicated reality they face due to illegality of sex work and multitude of complex healthcare experiences.