bluuebunny/crossref_metadata_2025 huggingface dataset:
Differential Microbial Pattern Description in Subjects with Autoimmune-Based Thyroid Diseases: A Pilot Study
Authors: Cornejo-Pareja Isabel , Ruiz-Limón Patricia , Gómez-Pérez Ana M. , Molina-Vega María , Moreno-Indias Isabel , Tinahones Francisco J.
Published: October 2020

The interaction between genetic susceptibility, epigenetic, endogenous, and environmental factors play a key role in the initiation and progression of autoimmune thyroid diseases (AITDs). Studies have shown that gut microbiota alterations take part in the development of autoimmune diseases. We have investigated the possible relationship between gut microbiota composition and the most frequent AITDs. A total of nine Hashimoto’s thyroiditis (HT), nine Graves–Basedow’s disease (GD), and 11 otherwise healthy donors (HDs) were evaluated. 16S rRNA pyrosequencing and bioinformatics analysis by Quantitative Insights into Microbial Ecology and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) were used to analyze the gut microbiota. Beta diversity analysis showed that gut microbiota from our groups was different. We observed an increase in bacterial richness in HT and a lower evenness in GD in comparison to the HDs. GD showed a significant increase of Fusobacteriaceae, Fusobacterium and Sutterella compared to HDs and the core microbiome features showed that Prevotellaceae and Prevotella characterized this group. Victivallaceae was increased in HT and was part of their core microbiome. Streptococcaceae, Streptococcus and Rikenellaceae were greater in HT compared to GD. Core microbiome features of HT were represented by Streptococcus, Alistipes, Anaerostipes, Dorea and Haemophilus. Faecalibacterium decreased in both AITDs compared to HDs. PICRUSt analysis demonstrated enrichment in the xenobiotics degradation, metabolism, and the metabolism of cofactors and vitamins in GD patients compared to HDs. Moreover, correlation studies showed that some bacteria were widely correlated with autoimmunity parameters. A prediction model evaluated a possible relationship between predominant concrete bacteria such as an unclassified genus of Ruminococcaceae, Sutterella and Faecalibacterium in AITDs. AITD patients present altered gut microbiota compared to HDs. These alterations could be related to the immune system development in AITD patients and the loss of tolerance to self-antigens.
Short-Term Sources of Cross-Linguistic Phonetic Influence: Examining the Role of Linguistic Environment
Authors: Olson Daniel J.
Published: October 2020

While previous research has shown that bilinguals are able to effectively maintain two sets of phonetic norms, these two phonetic systems experience varying degrees of cross-linguistic influence, driven by both long-term (e.g., proficiency, immersion) and short-term (e.g., bilingual language contexts, code-switching, sociolinguistic) factors. This study examines the potential for linguistic environment, or the language norms of the broader community in which an interaction takes place, to serve as a source of short-term cross-linguistic phonetic influence. To investigate the role of linguistic environment, late bilinguals (L1 English—L2 Spanish) produced Spanish utterances in two sessions that differed in their linguistic environments: an English-dominant linguistic environment (Indiana, USA) and a Spanish-dominant linguistic environment (Madrid, Spain). Productions were analyzed at the fine-grained acoustic level, through an acoustic analysis of voice onset time, as well as more holistically through native speaker global accent ratings. Results showed that linguistic environment did not significantly impact either measure of phonetic production, regardless of a speaker’s second language proficiency. These results, in conjunction with previous results on long- and short-term sources of phonetic influence, suggest a possible primacy of the immediate context of an interaction, rather than broader community norms, in determining language mode and cross-linguistic influence.
Staphylococcus epidermidis Biofilms Have a High Tolerance to Antibiotics in Periprosthetic Joint Infection
Authors: Koch John A. , Pust Taylor M. , Cappellini Alex J. , Mandell Jonathan B. , Ma Dongzhu , Shah Neel B. , Brothers Kimberly M. , Urish Kenneth L.
Published: October 2020

Both Staphylococcus aureus and Staphylococcus epidermidis are commonly associated with periprosthetic joint infections (PJIs). The treatment of PJI can be challenging because biofilms are assumed to have an increased intolerance to antibiotics. This makes the treatment of PJI challenging from a clinical perspective. Although S. aureus has been previously demonstrated to have increased biofilm antibiotic tolerance, this has not been well established with Staphylococcus epidermidis. A prospective registry of PJI S. epidermidis isolates was developed. The efficacy of clinically relevant antibiotics was quantified against these isolates. S. epidermidis planktonic minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were collected using clinical laboratory standard index (CLSI) assays for eight antibiotics (doxycycline, vancomycin, daptomycin, clindamycin, rifampin, nafcillin, and trimethoprim/sulfamethoxazole). Mature biofilms were grown in vitro, after which minimum biofilm inhibitory concentration (MBIC) and minimum biofilm bactericidal concentration (MBBC) were quantified. Only rifampin and doxycycline had a measurable MBIC across all tested isolates. Based on MBBC, 64% of S. epidermidis biofilms could be eliminated by rifampin, whereas only 18% by doxycycline. S. epidermidis biofilm was observed to have a high tolerance to antibiotics as compared to planktonic culture. Isolate biofilm antibiotic tolerance varied to a larger degree than was seen in planktonic cultures.
Blockchain Technology in the Food Industry: A Review of Potentials, Challenges and Future Research Directions
Authors: Rejeb Abderahman , Keogh John G. , Zailani Suhaiza , Treiblmaier Horst , Rejeb Karim
Published: October 2020

Blockchain technology has emerged as a promising technology with far-reaching implications for the food industry. The combination of immutability, enhanced visibility, transparency and data integrity provides numerous benefits that improve trust in extended food supply chains (FSCs). Blockchain can enhance traceability, enable more efficient recall and aids in risk reduction of counterfeits and other forms of illicit trade. Moreover, blockchain can enhance the integrity of credence claims such as sustainably sourced, organic or faith-based claims such as kosher or halal by integrating the authoritative source of the claim (e.g., the certification body or certification owner) into the blockchain to verify the claim integrity and reassure business customers and end consumers. Despite the promises and market hype, a comprehensive overview of the potential benefits and challenges of blockchain in FSCs is still missing. To bridge this knowledge gap, we present the findings from a systematic review and bibliometric analysis of sixty-one (61) journal articles and synthesize existing research. The main benefits of blockchain technology in FCSs are improved food traceability, enhanced collaboration, operational efficiencies and streamlined food trading processes. Potential challenges include technical, organizational and regulatory issues. We discuss the theoretical and practical implications of our research and present several ideas for future research.
Magnesia (MgO) Production and Characterization, and Its Influence on the Performance of Cementitious Materials: A Review
Authors: Nobre José , Ahmed Hawreen , Bravo Miguel , Evangelista Luís , de Brito Jorge
Published: October 2020

This paper presents a literature review concerning the characteristics of MgO (magnesium oxide or magnesia) and its application in cementitious materials. It starts with the characterization of MgO in terms of production processes, calcination temperatures, reactivity, and physical properties. Relationships between different MgO characteristics are established. Then, the influence of MgO incorporation on the properties of cementitious materials is investigated. The mechanical strength and durability behaviour of cement pastes, mortars and concrete mixes made with MgO are discussed. The studied properties of MgO–cement mixes include compressive strength, flexural strength, tensile strength, modulus of elasticity, water absorption, porosity, carbonation, chloride ion penetration, shrinkage, expansion, and hydration degree. In addition, microscopic analyses of MgO-cement mixes are also assessed. Summarizing the results of different studies, it is concluded that MgO incorporation in cementitious materials generally decreases the mechanical strength and shrinkage, and increases the porosity, expansion, carbonation and chloride ion migration. However, it should be emphasized that the properties of the specific MgO used (mainly the calcination temperature, the reactivity and the surface area) have a significant influence on the characteristics of the cementitious materials produced.
Propagating Surface Plasmon Polaritons on Systems with Variable Periodicity and Variable Gap-Depth
Authors: O’Toole Silas , Zerulla Dominic
Published: October 2020

Here we report on both simulations and experimental results on propagation and transmission of Surface Plasmon Polaritons (SPPs) through tunable gaps which were initially motivated by excitation of SPPs on a periodic arrangement of nanowires with mechanically tuneable periodicity. The general ability to vary the two-dimensional lattice constant results in an additional degree of freedom, permitting excitation of SPP’s for any combination of wavelength and angle of incidence within the tuning range of the system. Fabrication of the tunable system includes a transition from a continuously metal coated surface to small metal ribbons which can be separated from each other as a result of mechanical strain applied to the flexible PDMS substrate. This also results in the creation of tuneable gaps between the metal ribbons and variations in the thickness of the metal coatings. In order to explain the propagation of SPPs through such gaps we have employed Finite Difference Time Domain (FDTD) simulations of SPPs through model systems which contain gaps with varying depths and metal fillings.
Effect of Honeycomb β-TCP Geometrical Structure on Bone Tissue Regeneration in Skull Defect
Authors: Watanabe Toshiyuki , Takabatake Kiyofumi , Tsujigiwa Hidetsugu , Watanabe Satoko , Nakagiri Ryoko , Nakano Keisuke , Nagatsuka Hitoshi , Kimata Yoshihiro
Published: October 2020

The effect of the geometric structure of artificial biomaterials on skull regeneration remains unclear. In a previous study, we succeeded in developing honeycomb β-tricalcium phosphate (β-TCP), which has through-and-through holes and is able to provide the optimum bone microenvironment for bone tissue regeneration. We demonstrated that β-TCP with 300-μm hole diameters induced vigorous bone formation. In the present study, we investigated how differences in hole directions of honeycomb β-TCP (horizontal or vertical holes) influence bone tissue regeneration in skull defects. Honeycomb β-TCP with vertical and horizontal holes was loaded with BMP-2 using Matrigel and Collagen gel as carriers, and transplanted into skull bone defect model rats. The results showed that in each four groups (Collagen alone group, Matrigel alone group, Collagen + BMP group and Matrigel + BMP-2), vigorous bone formation was observed on the vertical β-TCP compared with horizontal β-TCP. The osteogenic area was larger in the Matrigel groups (with and without BMP-2) than in the Collagen group (with and without BMP-2) in both vertical β-TCP and horizontal β-TCP. However, when BMP-2 was added, the bone formation area was not significantly different between the Collagen group and the Matrigel group in the vertical β-TCP. Histological finding showed that, in vertical honeycomb β-TCP, new bone formation extended to the upper part of the holes and was observed from the dura side to the periosteum side as added to the inner walls of the holes. Therefore, we can control efficient bone formation by creating a bone microenvironment provided by vertical honeycomb β-TCP. Vertical honeycomb β-TCP has the potential to be an excellent biomaterial for bone tissue regeneration in skull defects and is expected to have clinical applications.
Effect of APPT Treatment on Mechanical Properties and Durability of Green Composites with Woven Flax
Authors: Enciso Belén , Abenojar Juana , Martínez Miguel Angel
Published: October 2020

Through this study, two different natural fibres green composites were characterised from the point of view of mechanical properties and durability. These green polymers allow manufacturing with a respectful life cycle due to their biodegradable or recyclable character. Composite materials were prepared in a hot plates press with two biopolymeric matrices, green low density polyethylene (GPE) and polybutylene succinate (PBS). As reinforcement, Atmospheric Pressure Plasma Torch (APPT) treated and untreated unidirectional woven flax were used. Mechanical properties were evaluated by tensile tests and the adhesion between matrices and reinforcement by peeling tests. The durability of each composite was analysed by water absorption measurements, Fourier Transform Infrared Spectroscopy (FTIR) analysis and tensile tests, during several aging times, up to 60 days, under high temperature and humidity conditions. The influence of the Atmospheric Pressure Plasma Torch treatment (APPT) was evaluated in all studies. It was found that GPE composites present better durability against aging conditions than PBS materials, due to the tendency of polyester to hydrolyse compared to the good resistance to humidity of polyolefins. The adhesion between matrices and reinforcement improves with APPT treatment. This improvement is more evident by avoiding the absorption of water than in the mechanical properties results, where only a slightly improvement is shown.
Effects of Caries Activity on Compositions of Mutans Streptococci in Saliva-Induced Biofilm Formed on Bracket Materials
Authors: Lim Bum-Soon , Kim Bo-Hyun , Shon Won-Jun , Ahn Sug-Joon
Published: October 2020

This study aimed to investigate effects of caries activity on composition of mutans streptococci in saliva-induced biofilms formed on bracket materials. Three bracket materials were used as specimens: ceramic, metal, and plastic. After saliva was collected using a spitting method from caries-active (CA, decayed, missing, filled teeth (DMFT) score ≥ 10) and caries-free (CF, DMFT score = 0) subjects, saliva was mixed with growth media in a proportion of 1:10. The saliva solution was then incubated with each bracket material. After a saliva-induced biofilm was developed on the surface of the bracket material, the amounts of total bacteria and mutans streptococci were determined using real-time polymerase chain reaction. The results showed that biofilms from CA saliva contained more mutans streptococci but less total bacteria than biofilms from CF saliva, regardless of material type. Adhesion of total bacteria to ceramic was higher than to plastic, regardless of caries activity. Mutans streptococci adhered more to ceramic than to metal and plastic in both biofilms from CA and CF saliva, but there was a difference in adhesion between Streptococcus mutans and Streptococcus sobrinus. The amount of S. mutans was higher than that of S. sobrinus in biofilms from CA saliva despite similar amounts of the two strains in biofilms from CF saliva. The stronger adhesion of S. mutans to ceramic than to metal and plastic was more evident in biofilms from CA saliva than in biofilms from CF saliva. This study suggests that caries activity and material type significantly influenced composition of mutans streptococci in biofilms formed on bracket materials.
The Effect of Temperature and Humidity on the Filtration Performance of Electret Melt-Blown Nonwovens
Authors: Liu Chao , Dai Zijian , He Bin , Ke Qin-Fei
Published: October 2020

Electret melt-blown nonwovens are widely used for air purification due to their low pressure drop and high filtration efficiency. However, the charge stability could be affected by the ambient temperature and humidity, reducing the filtration efficiency, resulting in the electret melt blown filter not providing effective protection. Herein, we used corona charge to prepare electret melt-blown nonwovens and systematically studied the effects of different temperature and humidity on the structure, morphology, filtration performance, and surface potential within 24 h. The effect of treatment temperature and humidity on pressure drop was minimal because the fiber morphology and web structure of melt-blown nonwovens were not damaged. When the treatment temperature was lower than 70 °C, the effect on the filtration efficiency of the sample was small, but when the temperature increased to 90 or 110 °C, the filtration efficiency decreased significantly with the increase of the treatment time, and the surface potential also declined similarly. In conclusion, high temperatures will lead to charge escape and reduce the electrostatic adsorption effect. Furthermore, at the same temperature, increasing relative humidity can accelerate the charge release and make the filtration efficiency drop more. After the sample was treated at 110 °C and 90% relative humidity for 24 h, the filtration efficiency decreased from 95.49% to 38.16% at a flow rate of 14.16 cm s−1, and the surface potential dropped to the lowest value of −1.01 kV. This result shows that all links of electret melt-blown filter material from raw material to final use should be avoided in high temperature and high humidity conditions to ensure the protection effect.
Pseudoceratonic Acid and Moloka’iamine Derivatives from the Red Sea Verongiid Sponge Pseudoceratina arabica
Authors: Shaala Lamiaa A. , Youssef Diaa T. A.
Published: October 2020

During an investigation of the chemistry of the Red Sea Verongiid sponge Pseudoceratina arabica, we discovered a small molecule, pseudoceratonic acid (1), along with the new moloka’iamine derivatives, ceratinines N (2), O (3), and the previously reported compounds moloka’iamine (4), hydroxymoloka’iamine (5) and ceratinamine (6). The structural assignments of 1–6 were accomplished by interpretation of their NMR and HRESIMS spectral data. Pseudoceratonic acid possesses a dibrominated hydrazine-derived functional group not found in any reported chemical compound. Pseudoceratonic acid selectively inhibited the growth of E. coli and S. aureus, while ceratinine N selectively inhibited C. albicans. Further, ceratinine N showed potent cytotoxic effects against the triple-negative breast cancer, colorectal carcinoma, and human cervical carcinoma cell lines down to 2.1 µM.
Itching Caused by TRPV3 (Transient Receptor Potential Vanilloid-3) Activator Application to Skin of Burn Patients
Authors: Kim Hye One , Jin Cheol Kim , Yu Gyeong Kong , In Suk Kwak
Published: October 2020

Background and objectives: Postburn pruritus is a common complication of scars in burn patients. In our previous study, we discovered increased expression of TRPV3, TRPV4, and TRPA1. Among them, TRPV3, in particular, is predominantly expressed in the epidermis of the tissue of pruritic burn scars. We sought to evaluate the correlation between the expression of TRPV3 activators and itching after application of TRPV3 activator carvacrol over burn scars. Materials and Methods: Design: This was a double-blind clinical trial with non-randomized distribution. Setting: This study was performed in a hospital setting. Patients or participants: Patients with itching of burn scars (numerical rating scale (NRS), <3; n = 8; Group 1 and NRS, ≥3; n = 16, Group 2) and healthy volunteers (n = 18, Group 3) were included. Interventions: The investigational drug carvacrol (100%, 75%, and 50%) and control drug (10% ethanol) were applied over the scars using IQ Ultimate™ 1 × 1 cm chamber sheets. Main variables of interest: The presence of pruritus, erythema, edema, and other skin lesions was evaluated. The verbal NRS scores were also compared. Results: Carvacrol induced more itching in Group 2 than in Groups 1 and 3. In Group 2, 100%, 75%, and 50% carvacrol caused significantly more itching (NRS score, 5.18 ± 3.04, 5.18 ± 3.04, and 4.93 ± 2.96, respectively) than that in Group 1 (NRS score, 1.00 ± 1.80, 1.00 ± 1.80, and 1.00 ± 1.80, respectively) and Group 3 (NRS score, 2.38 ± 1.94, 1.27 ± 1.32, respectively). Conclusions: The TRPV3 activator carvacrol can cause itching if applied over burn scars. This study can help understand the mechanism and prognosis of post-burn itching and contribute to the development of its treatment.
Characterization of Sintered Aluminium Reinforced with Ultrafine Tungsten Carbide Particles
Authors: Emadinia Omid , Vieira Maria Teresa , Vieira Manuel Fernando
Published: October 2020

The strengthening effect on aluminium (Al) by ultrafine particles of tungsten carbide (WC) after compacting and sintering was evaluated. The Al-1 vol.% WC mixture was prepared through a high-speed stirring technique, called assisted sonication. In this study, the effects of compacting, sintering temperature and holding time were evaluated by composite microstructural characterization and by mechanical tests. The characterizations involved electron dispersive spectroscopy and X-ray diffraction techniques for phase identification; electron backscattered diffraction for crystallographic analysis; backscattered electrons and secondary electrons imaging for failure and wear studies. In all composites, hardness was determined; for the hardest composite, the tensile strength, flexural strength and ball scattering wear resistance were also evaluated. The Al-1 vol.% WC composite produced by assisted sonication, densified by cold compacting at 152 MPa and sintered at 640 °C for 2 h at 5 × 10−4 Pa (high vacuum) exhibited the highest hardness, associated with an acceptable ductile behavior. This strengthening was associated with the formation of Al12W and grain refinement.
Decrease of Nozzle Clogging through Fluid Flow Control
Authors: Gutiérrez Enif , Barreto Jose de Jesus , Garcia-Hernandez Saul , Morales Rodolfo , González-Solorzano María G.
Published: October 2020

Decreasing the clogging deposition rate of alumina inclusions in continuous casting nozzles is possible through three simultaneous measures: Flow modification, use of raw materials with low impurities contents, and smoothed internal surfaces. The control of the internal flow consists on avoiding dead regions and developing symmetric patterns. A mathematical model performed tests of the feasibility of these measures. The adherence of inclusions to the nozzle wall, using this model, employs a boundary condition based on the thickness of the sublaminar boundary instead of the conventional “trap” boundary condition. The use of the general boundary condition yields deposition rates that are unaffected by the inclusion size. The proposed boundary condition discriminates against the clogging deposition rate through the particle sizes. Plant trials complemented with water modeling, using these nozzles, proved that the present approach could considerably decrease the clogging occurrence.
Uranium Removal from Groundwater and Wastewater Using Clay-Supported Nanoscale Zero-Valent Iron
Authors: Kornilovych Borys , Kovalchuk Iryna , Tobilko Viktoriia , Ubaldini Stefano
Published: October 2020

The peculiarities of sorption removal of uranium (VI) compounds from the surface and mineralized groundwater using clay-supported nanoscale zero-valent iron (nZVI) composite materials are studied. Representatives of the main structural types of clay minerals are taken as clays: kaolinite (Kt), montmorillonite (MMT) and palygorskite (Pg). It was found that the obtained samples of composite sorbents have much better sorption properties for the removal of uranium from surface and mineralized waters compared to natural clays and nZVI.It is shown that in mineralized waters uranium (VI) is mainly in anionic form, namely in the form of carbonate complexes, which are practically not extracted by pure clays. According to the efficiency of removal of uranium compounds from surface and mineralized waters, composite sorbents form a sequence: montmorillonite-nZVI > palygorskite-nZVI > kaolinite-nZVI, which corresponds to a decrease in the specific surface area of the pristine clay minerals.
Integrative Metabolomic and Transcriptomic Analyses Reveal Metabolic Changes and Its Molecular Basis in Rice Mutants of the Strigolactone Pathway
Authors: Zhou Xiujuan , Liu Ling , Li Yufei , Li Kang , Liu Xiaoli , Zhou Junjie , Yang Chenkun , Liu Xianqing , Fang Chuanying , Luo Jie
Published: October 2020

Plants have evolved many metabolites to meet the demands of growth and adaptation. Although strigolactones (SLs) play vital roles in controlling plant architecture, their function in regulating plant metabolism remains elusive. Here we report the integrative metabolomic and transcriptomic analyses of two rice SL mutants, d10 (a biosynthesis mutant) and d14 (a perception mutant). Both mutants displayed a series of metabolic and transcriptional alterations, especially in the lipid, flavonoid, and terpenoid pathways. Levels of several diterpenoid phytoalexins were substantially increased in d10 and d14, together with the induction of terpenoid gene cluster and the corresponding upstream transcription factor WRKY45, an established determinant of plant immunity. The fact that WRKY45 is a target of IPA1, which acted as a downstream transcription factor of SL signaling, suggests that SLs contribute to plant defense through WRKY45 and phytoalexins. Moreover, our data indicated that SLs may modulate rice metabolism through a vast number of clustered or tandemly duplicated genes. Our work revealed a central role of SLs in rice metabolism. Meanwhile, integrative analysis of the metabolome and transcriptome also suggested that SLs may contribute to metabolite-associated growth and defense.
Experimental Tests on a Small-Scale Model of a Mine Stope to Study the Behavior of Waste Rock Barricades during Backfilling
Authors: Nujaim Mutaz , Belem Tikou , Giraud Albert
Published: October 2020

This paper presents a reduced-scale physical model of a mine stope used to reproduce the underground stope backfilling practiced in some Canadian mines. The objective is to study the geomechanical behavior of the waste rock barricades in interaction with the mine backfill. The instrumentations, along with visual observations and preliminary results, are presented. The main results demonstrated that: (i) the stability of the barricade depends on its physical properties (e.g., size, location, particles gradation and compaction) and the frictional behavior at the barricade/drift walls interface, and (ii) for two backfill formulations, cemented and uncemented, prepared with 70% of solid mass concentration, the volumetric strain due to self-weight consolidation of the backfilled room was higher for uncemented backfill (16%) than for the cemented one (4.5%). In addition, the results highlighted the importance of using shotcrete around the downstream face of the barricade, mostly at the top, to close the gaps and bind the barricade particles, which improves its stability.
Mineralogical Setting of Precious Metals at the Assarel Porphyry Copper-Gold Deposit, Bulgaria, as Supporting Information for the Development of New Drill Core 3D XCT-XRF Scanning Technology
Authors: Cioacă Mihaela-Elena , Munteanu Marian , Lynch Edward P. , Arvanitidis Nikolaos , Bergqvist Mikael , Costin Gelu , Ivanov Desislav , Milu Viorica , Arvidsson Ronald , Iorga-Pavel Adina , Högdahl Karin , Stoilov Ventsislav
Published: October 2020

A petrographic investigation of ore samples from the Assarel porphyry copper deposit in the Srednogorie metallogenic zone (Bulgaria) constrains the setting and character of precious metals (Au, Ag, PGE) and related minerals within the deposit. This work supports renewed interest in understanding the deportment of precious metals and provides mineralogical knowledge during the testing and validation of novel drill core 3D X-ray computed tomography–X-ray fluorescence (XCT-XRF) scanning technology being developed as part of the X-MINE project. Scanning electron microscopy–energy dispersive spectrometry (SEM-EDS) results indicate precious metals occur in their native state (Au, Ag), as sulfides (Ag), sulfosalts (Au), tellurides (Ag, Pd), and selenides (Ag), and typically form micron-sized inclusions in pyrite and chalcopyrite or are disseminated in the groundmass of the rock. Preservation of early Fe oxide–chalcopyrite ± bornite assemblage as relics in the more dominant pyrite-chalcopyrite mineralization assemblage supports mineral disequilibrium relationships and multi-stage mineralization events. Several rare minerals (e.g., merenskyite, acanthite, sorosite, tetra-auricupride, auricupride, greenokite, bismuthinite, nagyagite, native Ni) are reported for the first time at Assarel and highlight the mineralogical diversity of the ore. The occurrence of precious metals and related minerals at Assarel attest to a complex hydrothermal system that underwent progressive physicochemical changes during the evolution of the mineralizing system (e.g., redox conditions, fluid chemistry).
Crystal Chemistry and Structural Variations for Zircon Samples from Various Localities
Authors: Zaman M. , Antao Sytle
Published: October 2020

This study investigates the variations of structural parameters and chemistry of a partially metamict and seven detrital zircon samples from different localities using single-crystal X-ray diffraction, synchrotron high-resolution powder X-ray diffraction, and electron-probe micro-analysis techniques. The unit-cell parameters for the eight zircon samples vary linearly with increasing unit-cell volume, V. A zircon sample from the Canadian Arctic Islands has the smallest unit-cell parameters, bond distances, ideal stoichiometric composition, unaffected by α-radiation damage, and is chemically pure. A zircon sample from Jemaa, Nigeria has the largest unit-cell parameters because of the effect of α-radiation doses received over a long time (2384 Ma). All the samples show good correlations between Zr and Si apfu (atom per formula unit) versus unit-cell volume, V. The α-radiation doses in the samples are lower than ~3.5 × 1015 α-decay events/mg. Substitutions of other cations at the Zr and Si sites control the variations of the structural parameters. Relatively large unit-cell parameters and bond distances occur because the Zr site accommodates other cations that have larger ionic radii than the Zr atom. Geological age increases the radiation doses in zircon and it is related to V.
Economic and Socio-Environmental Benefits of Dry Beneficiation of Magnetite Ores
Authors: Baawuah Emmanuel , Kelsey Christopher , Addai-Mensah Jonas , Skinner William
Published: October 2020

In our previous studies, we demonstrated the performance of novel superfine crusher and pneumatic planar magnetic separator as energy-efficient technologies for dry processing of magnetite ores. The present study investigates the economic and socio-environmental benefits of applying these technologies in conceptual dry magnetite ore processing flowsheet. The outcome of the study is compared with that of a conceptual wet processing flowsheet for the same ore. The cost estimations used are based on the Brook Hunt C1 methodology whilst revenue estimations are based on the Platts Iron Ore Index specification. The demonstrated economic and socio-environmental benefits show that dry processing flowsheet offers significant energy and cost savings and improved revenue generation compared with the wet process flowsheet. These findings are vital to the magnetite industry, particularly in water- and energy-scarce regions as a benchmark for future studies aimed at deepening and expanding the knowledge base of dry beneficiation of magnetite ores.
Controlling Particle Morphology and Pore Size in the Synthesis of Ordered Mesoporous Materials
Authors: Awoke Yaregal , Chebude Yonas , Díaz Isabel
Published: October 2020

Ordered mesoporous materials have attracted considerable attention due to their potential applications in catalysis, adsorption, and separation technologies, as well as biomedical applications. In the present manuscript, we aim at a rational design to obtain the desired surface functionality (Ti and/or hydrophobic groups) while obtaining short channels (short diffusion paths) and large pore size (>10 nm). Santa Barbara Amorphous material SBA-15 and periodic mesoporous organosilica PMO materials are synthesized using Pluronic PE 10400 (P104) surfactant under mild acidic conditions to obtain hexagonal platelet-like particles with very short mesochannels (300–450 nm). The use of expanders, such as 1, 3, 5-trimethylbenzene (TMB) and 1, 3, 5-triisopropylbenzene (TIPB) were tested in order to increase the pore size. TMB yielded in the formation of vesicles in all the syntheses attempted, whereas P104 combined with TIPB resulted both in expanded (E) E-SBA-15 and E-PMO with 12.3 nm pore size short channel particles in both cases. Furthermore, the synthesis method was expanded to the incorporation of small amount of Ti via co-condensation method using titanocene as titanium source. As a result, Ti-E-SBA-15 was obtained with 15.5 nm pore size and isolated Ti-sites maintaining platelet hexagonal morphology. Ti-PMO was obtained with 7.8 nm and short channels, although the pore size under the tried synthesis conditions could not be expanded further without losing the structural ordering.
Tannin-Based Hybrid Materials and Their Applications: A Review
Authors: Koopmann Ann-Kathrin , Schuster Christian , Torres-Rodríguez Jorge , Kain Stefan , Pertl-Obermeyer Heidi , Petutschnigg Alexander , Hüsing Nicola
Published: October 2020

Tannins are eco-friendly, bio-sourced, natural, and highly reactive polyphenols. In the past decades, the understanding of their versatile properties has grown substantially alongside a continuously broadening of the tannins’ application scope. In particular, recently, tannins have been increasingly investigated for their interaction with other species in order to obtain tannin-based hybrid systems that feature advanced and/or novel properties. Furthermore, in virtue of the tannins’ chemistry and their high reactivity, they either physicochemically or physically interact with a wide variety of different compounds, including metals and ceramics, as well as a number of organic species. Such hybrid or hybrid-like systems allow the preparation of various advanced nanomaterials, featuring improved performances compared to the current ones. Consequently, these diverse-shaped materials have potential use in wastewater treatment or catalysis, as well as in some novel fields such as UV-shielding, functional food packaging, and biomedicine. Since these kinds of tannin-based hybrids represent an emerging field, thus far no comprehensive overview concerning their potential as functional chemical building blocks is available. Hence, this review aims to provide a structured summary of the current state of research regarding tannin-based hybrids, detailed findings on the chemical mechanisms as well as their fields of application.
Isolation and Characterization of Phenylpropanoid and Lignan Compounds from Peperomia pellucida [L.] Kunth with Estrogenic Activities
Authors: Kartika I Gusti Agung Ayu , Bang In Jae , Riani Catur , Insanu Muhamad , Kwak Jong Hwan , Chung Kyu Hyuck , Adnyana I Ketut
Published: October 2020

Extracts of Peperomia pellucida [L.] Kunth have previously been demonstrated to have in vivo estrogenic-like effects, thereby functioning as an anti-osteoporotic agent. However, the compounds responsible for these effects have not yet been determined. Therefore, the aim of this study is to isolate and elucidate potential compounds with estrogenic activity. The structures of the isolated compounds were identified using 1D 1H and 13C-NMR and confirmed by 2D FT-NMR. The estrogenic activity was evaluated using the E-SCREEN assay, and a molecular docking study was performed to predict the binding affinity of the isolated compounds to estrogen receptors. In this experiment, we successfully isolated three phenylpropanoids and two lignan derivatives, namely, 6-allyl-5-methoxy-1,3-benzodioxol-4-ol (1), pachypostaudin B (2), pellucidin A (3), dillapiole (4), and apiol (5). Among these compounds, the isolation of 1 and 2 from P. pellucida is reported for the first time in this study. Activity assays clearly showed that the ethyl acetate extract and its fractions, subfractions, and isolated compounds exerted estrogenic activity. Methanol fraction of the ethyl acetate extract produced the highest estrogenic activity, while 1 and 2 had partial agonist activity. Some compounds (derivates of dillapiole and pellucidin A) also had, in addition, anti-estrogenic activity. In the docking study, the estrogenic activities of 1–5 appeared to be mediated by a classical ligand-dependent mechanism as suggested by the binding interaction between the compounds and estrogen receptors; binding occurred on Arg 394 and His 524 of the alpha receptor and Arg 346 and His 475 of the beta receptor. In summary, we reveal that P. pellucida is a promising anti-osteoporotic agent due to its estrogenic activity, and the compounds responsible for this activity were found to be lignan and phenylpropanoid derivatives. The presence of other compounds in either the extract or fraction may contribute to a synergistic effect, as suggested by the higher estrogenic activity of the methanol fraction. Hence, we suggest further research on the osteoporotic activity and safety of the identified compounds, especially regarding their effects on estrogen-responsive organs.
Catechol-Type Flavonoids from the Branches of Elaeagnus glabra f. oxyphylla Exert Antioxidant Activity and an Inhibitory Effect on Amyloid-β Aggregation
Authors: Kim Yu Jin , Sohn Eunjin , Kim Joo-Hwan , Na MinKyun , Jeong Soo-Jin
Published: October 2020

Elaeagnus glabra f. oxyphylla (Elaeagnaceae) is a small evergreen tree with narrow lanceolate leaves that is native to Korea. In this work, we studied the chemical composition of E. glabra f. oxyphylla branches (EGFOB) for the first time. Additionally, we evaluated the effects of the ethanol extract of EGFOB and each of its chemical components on key mediators of Alzheimer’s disease (AD), namely, amyloid-β (Aβ) aggregation and oxidative stress. The ethanol extract of EGFOB decreased Aβ aggregation (IC50 = 32.01 µg/mL) and the levels of the oxidative free radicals 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 11.35 and 12.32 µg/mL, respectively). Sixteen compounds were isolated from EGFOB. Among them, procyanidin B3 (8), procyanidin B4 (9), and helichrysoside (13) significantly inhibited Aβ aggregation (IC50 = 14.59, 32.64, and 44.45 μM, respectively), indicating their potential as bioactive compounds to control Aβ aggregation. Furthermore, these compounds markedly enhanced in vitro scavenging activity against ABTS (IC50 = 3.21–4.61 µM). In the DPPH test, they showed lower scavenging activity than in the ABTS test (IC50 ≥ 54.88 µM). Thus, these results suggest that EGFOB and specifically compounds 8, 9, and 13 may be beneficial in AD prevention and treatment through their antioxidant and anti-Aβ aggregation activities.
Crosslinking of Polylactide by High Energy Irradiation and Photo-Curing
Authors: Bednarek Melania , Borska Katarina , Kubisa Przemysław
Published: October 2020

Polylactide (PLA) is presently the most studied bioderived polymer because, in addition to its established position as a material for biomedical applications, it can replace mass production plastics from petroleum. However, some drawbacks of polylactide such as insufficient mechanical properties at a higher temperature and poor shape stability have to be overcome. One of the methods of mechanical and thermal properties modification is crosslinking which can be achieved by different approaches, both at the stage of PLA-based materials synthesis and by physical modification of neat polylactide. This review covers PLA crosslinking by applying different types of irradiation, i.e., high energy electron beam or gamma irradiation and UV light which enables curing at mild conditions. In the last section, selected examples of biomedical applications as well as applications for packaging and daily-use items are presented in order to visualize how a variety of materials can be obtained using specific methods.
Label-Free Proteomics Reveals the Molecular Mechanism of Subculture Induced Strain Degeneration and Discovery of Indicative Index for Degeneration in Pleurotus ostreatus
Authors: Zhu Weiwei , Hu Jinbo , Chi Jingliang , Li Yang , Yang Bing , Hu Wenli , Chen Fei , Xu Chong , Chai Linshan , Bao Yongming
Published: October 2020

Pleurotus ostreatus is one of the widely cultivated edible fungi across the world. Mycelial subculture is an indispensable part in the process of cultivation and production for all kinds of edible fungi. However, successive subcultures usually lead to strain degeneration. The degenerated strains usually have a decrease in stress resistance, yield, and an alteration in fruiting time, which will subsequently result in tremendous economic loss. Through proteomic analysis, we identified the differentially expressed proteins (DEPs) in the mycelium of Pleurotus ostreatus from different subcultured generations. We found that the DNA damage repair system, especially the double-strand breaks (DSBs), repairs via homologous recombination, was impaired in the subcultured mycelium, and gradual accumulation of the DSBs would lead to the strain degeneration after successive subculture. The TUNEL assay further confirmed our finding about the DNA breaks in the subcultured mycelium. Interestingly, the enzyme activity of laccase, carboxylic ester hydrolase, α-galactosidase, and catalase directly related to passage number could be used as the characteristic index for strain degeneration determination. Our results not only reveal for the first time at the molecular level that genomic instability is the cause of degeneration, but also provide an applicable approach for monitoring strain degeneration in process of edible fungi cultivation and production.
Drying Techniques and Storage: Do They Affect the Nutritional Value of Bee-Collected Pollen?
Authors: Castagna Antonella , Benelli Giovanni , Conte Giuseppe , Sgherri Cristina , Signorini Francesca , Nicolella Cristiano , Ranieri Annamaria , Canale Angelo
Published: October 2020

In this study, the effect of different drying processes (freeze-drying (FD), microwave-assisted drying (MWD) and classic hot air drying (HAD)) on the polyphenols, flavonoids, and amino acids content was investigated on bee-collected chestnut, willow and ivy pollen for human consumption. Furthermore, the pollen chemical properties were monitored after three and six months of storage, and then analyzed using a multivariate approach. Chestnut pollen was the richest source of polyphenols, flavonoids, and rutin, while ivy pollen contained the highest amount of total and free amino acids, and total and free proline. Drying and storage affected pollen chemical composition with species-dependent effects. MWD allowed the best retention of flavonoids in chestnut pollen for up to six months of storage. All drying techniques led to a depletion of flavonoids in willow pollen; however, MWD ensured the highest flavonoids content after six months. FD and MWD did not lead to flavonoids depletion in ivy pollen during storage. Additionally, storage did not affect the rutin content, which was highest in FD willow samples after six months. Notably, both FD and MWD techniques are efficient in preserving amino acids-related quality of bee pollen up to six months of storage.
Modeling of the Production of Lipid Microparticles Using PGSS® Technique
Authors: López-Iglesias Clara , López Enriqueta R. , Fernández Josefa , Landin Mariana , García-González Carlos A.
Published: October 2020

Solid lipid microparticles (SLMPs) are attractive carriers as delivery systems as they are stable, easy to manufacture and can provide controlled release of bioactive agents and increase their efficacy and/or safety. Particles from Gas-Saturated Solutions (PGSS®) technique is a solvent-free technology to produce SLMPs, which involves the use of supercritical CO2 (scCO2) at mild pressures and temperatures for the melting of lipids and atomization into particles. The determination of the key processing variables is crucial in PGSS® technique to obtain reliable and reproducible microparticles, therefore the modelling of SLMPs production process and variables control are of great interest to obtain quality therapeutic systems. In this work, the melting point depression of a commercial lipid (glyceryl monostearate, GMS) under compressed CO2 was studied using view cell experiments. Based on an unconstrained D-optimal design for three variables (nozzle diameter, temperature and pressure), SLMPs were produced using the PGSS® technique. The yield of production was registered and the particles characterized in terms of particle size distribution. Variable modeling was carried out using artificial neural networks and fuzzy logic integrated into neurofuzzy software. Modeling results highlight the main effect of temperature to tune the mean diameter SLMPs, whereas the pressure-nozzle diameter interaction is the main responsible in the SLMPs size distribution and in the PGSS® production yield.
Silk Fibroin-Based Materials for Catalyst Immobilization
Authors: Lv Shanshan
Published: October 2020

Silk fibroin is a widely and commercially available natural protein derived from silkworm cocoons. Thanks to its unique amino acid composition and structure, which lead to localized nanoscale pockets with limited but sufficient hydration for protein interaction and stabilization, silk fibroin has been studied in the field of enzyme immobilization. Results of these studies have demonstrated that silk fibroin offers an important platform for covalent and noncovalent immobilization of enzymes through serving as a stabilization matrix/support with high retention of the biological activity of the enzymes of interest. In the hope of providing suggestions for potential future research directions, this review has been written to briefly introduce and summarize key advances in silk fibroin-based materials for immobilization of both enzymes/biocatalysts (including alkaline phosphatase, β-glucosidase, glucose oxidase, lipase, urease, uricase, horseradish peroxidase, catalase, xanthine oxidase, tyrosinase, acetylcholinesterase, neutral protease, α-chymotrypsin, amylase, organophosphorus hydrolase, β-galactosidase, carbonic anhydrase, laccase, zymolyase, phenylalanine ammonia-lyase, thymidine kinase, and several others) and non-enzymatic catalysts (such as Au, Pd, Fe, α-Fe2O3, Fe3O4, TiO2, Pt, ZnO, CuO, Cu2O, Mn3O4, and MnO2).