bluuebunny/crossref_metadata_2025 huggingface dataset:
Food Safety and Nutraceutical Potential of Caramel Colour Class IV Using In Vivo and In Vitro Assays
Authors: Mateo-Fernández Marcos , Alves-Martínez Pilar , Del Río-Celestino Mercedes , Font Rafael , Merinas-Amo Tania , Alonso-Moraga Ángeles
Published: September 2019

Nutraceutical activity of food is analysed to promote the healthy characteristics of diet where additives are highly used. Caramel is one of the most worldwide consumed additives and it is produced by heating natural carbohydrates. The aim of this study was to evaluate the food safety and the possible nutraceutical potential of caramel colour class IV (CAR). For this purpose, in vivo toxicity/antitoxicity, genotoxicity/antigenotoxicity and longevity assays were performed using the Drosophila melanogaster model. In addition, cytotoxicity, internucleosomal DNA fragmentation, single cell gel electrophoresis and methylation status assays were conducted in the in vitro HL-60 human leukaemia cell line. Our results reported that CAR was neither toxic nor genotoxic and showed antigenotoxic effects in Drosophila. Furthermore, CAR induced cytotoxicity and hipomethylated sat-α repetitive element using HL-60 cell line. In conclusion, the food safety of CAR was demonstrated, since Lethal Dose 50 (LD50) was not reached in toxicity assay and any of the tested concentrations induced mutation rates higher than that of the concurrent control in D. melanogaster. On the other hand, CAR protected DNA from oxidative stress provided by hydrogen peroxide in Drosophila. Moreover, CAR showed chemopreventive activity and modified the methylation status of HL-60 cell line. Nevertheless, much more information about the mechanisms of gene therapies related to epigenetic modulation by food is necessary.
Genome-Wide Identification and Expression Analysis of GA2ox, GA3ox, and GA20ox Are Related to Gibberellin Oxidase Genes in Grape (Vitis vinifera L.)
Authors: He Honghong , Liang Guoping , Lu Shixiong , Wang Pingping , Liu Tao , Ma Zonghuan , Zuo Cunwu , Sun Xiaomei , Chen Baihong , Mao Juan
Published: September 2019

Gibberellin (GAs) plays the important role in the regulation of grape developmental and growth processes. The bioinformatics analysis confirmed the differential expression of GA2, GA3, and GA20 gibberellin oxidase genes (VvGA2oxs, VvGA3oxs, and VvGA20oxs) in the grape genome, and laid a theoretical basis for exploring its role in grape. Based on the Arabidopsis GA2oxs, GA3oxs, and GA20oxs genes already reported, the VvGA2oxs, VvGA3oxs, and VvGA20oxs genes in the grape genome were identified using the BLAST software in the grape genome database. Bioinformatics analysis was performed using software such as DNAMAN v.5.0, Clustalx, MapGene2Chrom, MEME, GSDS v.2.0, ExPASy, DNAsp v.5.0, and MEGA v.7.0. Chip expression profiles were generated using grape Affymetrix GeneChip 16K and Grape eFP Browser gene chip data in PLEXdb. The expression of VvGA2oxs, VvGA3oxs, and VvGA20oxs gene families in stress was examined by qRT-PCR (Quantitative real-time-PCR). There are 24 GAoxs genes identified with the grape genome that can be classified into seven subgroups based on a phylogenetic tree, gene structures, and conserved Motifs in our research. The gene family has higher codon preference, while selectivity is negative selection of codon bias and selective stress was analyzed. The expression profiles indicated that the most of VvGAox genes were highly expressed under different time lengths of ABA (Abscisic Acid) treatment, NaCl, PEG and 5 °C. Tissue expression analysis showed that the expression levels of VvGA2oxs and VvGA20oxs in different tissues at different developmental stages of grapes were relatively higher than that of VvGA3oxs. Last but not least, qRT-PCR (Real-time fluorescent quantitative PCR) was used to determine the relative expression of the GAoxs gene family under the treatment of GA3 (gibberellin 3) and uniconazole, which can find that some VvGA2oxs was upregulated under GA3 treatment. Simultaneously, some VvGA3oxs and VvGA20oxs were upregulated under uniconazole treatment. In a nutshell, the GA2ox gene mainly functions to inactivate biologically active GAs, while GA20ox mainly degrades C20 gibberellins, and GA3ox is mainly composed of biologically active GAs. The comprehensive analysis of the three classes of VvGAoxs would provide a basis for understanding the evolution and function of the VvGAox gene family in a grape plant.
Transcript Abundance Patterns of 9- and 13-Lipoxygenase Subfamily Gene Members in Response to Abiotic Stresses (Heat, Cold, Drought or Salt) in Tomato (Solanum lycopersicum L.) Highlights Member-Specific Dynamics Relevant to Each Stress
Authors: Upadhyay Rakesh K. , Handa Avtar K. , Mattoo Autar K.
Published: September 2019

Lipoxygenases (LOXs; EC 1.13.11.12) catalyze the oxygenation of fatty acids to produce oxylipins including the jasmonate family of plant hormones. The involvement of jasmonates in plant growth and development and during abiotic stress has been documented, however, the response and regulation of each member of the LOX gene family under various abiotic stresses is yet to be fully deciphered. Previously, we identified fourteen members of the tomato LOX gene family, which were divisible into nine genes representing the 9-LOX family members and five others representing the 13-LOX family members based on the carbon oxidation position specificity of polyunsaturated fatty acids. Here, we have determined the transcript abundance patterns of all the 14 LOX genes in response to four independent abiotic stresses, namely, heat, cold, drought and salt. Our results show that each of these stresses leads to a time-dependent, variable or indifferent response of specific and different set(s) of LOX gene members of both subfamilies, differentiating functional relevance of the 14 LOX genes analyzed. Out of the 14 gene members, three LOX genes were expressed constitutively or were non-responsive to either heat (SlLOX9), cold (SlLOX9) or salt (SlLOX4) stress. An in-silico LOX gene promoter search for stress-responsive elements revealed that only some but not all of the LOX genes indeed are decorated with specific and known stress responsive cis-acting elements. Thus, these data implicate some other, yet to be discovered, cis-acting elements present in the LOX gene family members, which seemingly regulate tomato responses to defined abiotic stresses presented here.
Society and Culture: Cultural Policies Driven by Local Authorities as A Factor in Local Development—The Example of the Municipality of Xanthi-Greece
Authors: Papazoglou Grammatiki (Emmy)
Published: September 2019

This research article examines cultural policies designed by local government authorities and their impact on social and regional development in the municipality of Xanthi, Thrace. It also analyzes and examines the cultural activities implemented by the Greek municipalities. In particular, it reflects upon events, changes, and concerns that involve cultural affairs, and evaluates their socioeconomic, political, spatial, and regional dimensions. Considering that the locality is part of the totality, the process of achieving cultural development in Xanthi is particularly interesting, as it is fundamental to the entire Greek cultural image. The regional element of the area can be also identified as national. Consequently, local cultural development becomes an essential part of national development. This study could trigger a fertile and constructive process of reflection on the role of local cultural policy in further achieving social and economic development. The issues raised by the research contribute to scientific research and dialogue and highlight the role of municipalities as active cultural assets with distinct cultural identities in the context of a Europe of Regions.
Valuation of Estimation Toxic Chemical Release Inventory Method-Focusing on Paint Manufacturing Process
Authors: Lee Hyo Eun , Huh Eun-Hae , Yoon Yi , Yoon Seok J. , Huh Da-An , Moon Kyong Whan
Published: September 2019

Industrial chemicals differ in their treatment methods and types, depending on their physicochemical properties. Highly volatile chemicals are emitted despite installation of preventive facilities, such as scrubbers and adsorption towers. Some countries release a Toxic Release Inventory (TRI), which is a mandatory report on the amount of chemicals emitted annually. This report is released to the citizens to ensure their right to knowledge and life. Numerous methods have been devised to investigate the amount of chemical emissions. There are four methods to estimate TRI emissions (Emission Factor Method; Material Balance Method; Source Testing Method; Emission Model Method). Moreover, efforts have been made to increase awareness and formulate plans to reduce chemical emissions. Despite this, the TRI method tends to underestimate and overestimate, especially due to volatile compounds. If the results of the TRI emissions are underestimated, toxic chemicals can have a negative impact on citizens. Volatile compounds are commonly used in chemical manufacturing plants, such as paint plants. In this study, a suitable method for each industrial process was suggested based on conservative estimates of multiple toxic chemical inventory method, focusing on the paint manufacturing process. In the paint manufacturing plant, storage, weighing, and mixing processes should be used emission model method to estimate TRI. In the reaction process, TRI must be estimated by the source test method. In the transfer process, the emission factor method should be used to estimate TRI. In the atmosphere prevention process, the emission factor method or source testing method should be used depending on the physical and chemical properties such as vapor pressure of the chemical.
A Case of Incomplete and Atypical Kawasaki Disease Presenting with Retropharyngeal Involvement
Authors: Isidori Chiara , Sebastiani Lisa , Esposito Susanna
Published: September 2019

Background: Kawasaki disease (KD) is a childhood acute febrile vasculitis of unknown aetiology. The diagnosis is based on clinical criteria, including unilateral cervical lymphadenopathy, which is the only presenting symptom associated with fever in 12% of cases. A prompt differential diagnosis distinguishing KD from infective lymphadenitis is therefore necessary to avoid incorrect and delayed diagnosis and the risk of cardiovascular sequelae. Case presentation: We describe the case of a 4 years old boy presenting with febrile right cervical lymphadenopathy, in which the unresponsiveness to broad-spectrum antibiotics, the following onset of other characteristic clinical features and the evidence on the magnetic resonance imaging (MRI) of retropharyngeal inflammation led to the diagnosis of incomplete and atypical KD. On day 8 of hospitalisation (i.e., 13 days after the onset of symptoms), one dose of intravenous immunoglobulins (IVIG; 2 g/kg) was administered with rapid defervescence, and acetylsalicylic acid (4 mg/kg/day) was started and continued at home for a total of 8 weeks. Laboratory examinations revealed a reduction in the white blood cell count and the levels of inflammatory markers, thrombocytosis, and persistently negative echocardiography. Clinically, we observed a gradual reduction of the right-side neck swelling. Fifteen days after discharge, the MRI of the neck showed a regression of the laterocervical lymphadenopathy and a resolution of the infiltration of the parapharyngeal and retropharyngeal spaces. Conclusion: Head and neck manifestations can be early presentations of KD, which is frequently misdiagnosed as suppurative lymphadenitis or retropharyngeal infection. A growing awareness of the several possible presentations of KD is therefore necessary. Computed tomography (CT) or MRI can be utilised to facilitate the diagnosis.
The Influence of Forest Resting Environments on Stress Using Virtual Reality
Authors: Wang Xiaobo , Shi Yaxing , Zhang Bo , Chiang Yencheng
Published: September 2019

To explore the effects of different types of forest environments for forest therapy, this study focused on forest resting environments. Seven representative forest resting environments found in field research in Beijing were used as independent variables and were shown to subjects by a virtual reality (VR) video. Stress level was used as the dependent variable, and blood pressure, heart rate, salivary amylase, and the Brief Profile of Mood States (BPOMS) were used as physiological and psychological indicators. A between-subjects design was used in the experiment. A total of 96 subjects were randomly assigned to each environment type, and only one type of forest resting environment was observed. Through the relevant sample t-test and one-way analysis of variance, the pre- and post-test data of the indicators were analyzed. This study found that all the seven different types of forest resting environments can produce stress relief effects to some extent. Different types of forest resting environments have different effects on relieving stress. The most natural environment does not have the most significant effect on stress relief. A water landscape has a positive effect on the relief of stress. The conclusions of this study are conducive to the better use of the forest environment for forest therapy services.
Probabilistic Human Health Risk Assessment of Heavy Metal Intake via Vegetable Consumption around Pb/Zn Smelters in Southwest China
Authors: Guo Guanghui , Zhang Degang , Wang Yuntao
Published: September 2019

Vegetable contamination in mining and smelting areas has resulted in high dietary intakes of heavy metals, which pose potential health risks to local residents. In this study, paired soil-vegetable samples were collected around Pb/Zn smelters in Southwest China. Probabilistic risks to local residents via vegetable consumption were evaluated with a Monte Carlo simulation. The mean concentrations of As, Cd, Cu, Pb, and Zn in the soils were 116.76, 3.59, 158.56, 196.96, and 236.74 mg/kg, respectively. About 38.18%, 58.49%, and 52.83% of the vegetable samples exceeded the maximum allowable concentrations for As, Cd, and Pb, respectively. The daily dietary intake of As, Cd, and Pb exceeded the provisional tolerable daily intakes for local residents, with children showing the highest intake via vegetable consumption. The percentages of the target hazard quotients of As, Cd, and Pb for local residents exceeding the safe value of one were about 95%, 50%, and 25%, respectively. The 95th percentiles of the hazard index for children, adolescents, and adults were 15.71, 11.15, and 9.34, respectively, indicating significant risks to local residents, especially children. These results highlight a need to develop effective strategies to reduce heavy metal contamination and exposure to protect human health.
A Molecular Dynamics Study of Vasoactive Intestinal Peptide Receptor 1 and the Basis of Its Therapeutic Antagonism
Authors: Latek Dorota , Langer Ingrid , Krzysko Krystiana , Charzewski Lukasz
Published: September 2019

Vasoactive intestinal peptide receptor 1 (VPAC1) is a member of a secretin-like subfamily of G protein-coupled receptors. Its endogenous neuropeptide (VIP), secreted by neurons and immune cells, modulates various physiological functions such as exocrine and endocrine secretions, immune response, smooth muscles relaxation, vasodilation, and fetal development. As a drug target, VPAC1 has been selected for therapy of inflammatory diseases but drug discovery is still hampered by lack of its crystal structure. In this study we presented the homology model of this receptor constructed with the well-known web service GPCRM. The VPAC1 model is composed of extracellular and transmembrane domains that form a complex with an endogenous hormone VIP. Using the homology model of VPAC1 the mechanism of action of potential drug candidates for VPAC1 was described. Only two series of small-molecule antagonists of confirmed biological activity for VPAC1 have been described thus far. Molecular docking and a series of molecular dynamics simulations were performed to elucidate their binding to VPAC1 and resulting antagonist effect. The presented work provides the basis for the possible binding mode of VPAC1 antagonists and determinants of their molecular recognition in the context of other class B GPCRs. Until the crystal structure of VPAC1 will be released, the presented homology model of VPAC1 can serve as a scaffold for drug discovery studies and is available from the author upon request.
Inhibition of Mitochondrial Complex I Impairs Release of α-Galactosidase by Jurkat Cells
Authors: Lambert Jonathan , Howe Steven , Rahim Ahad , Burke Derek , Heales Simon
Published: September 2019

Fabry disease (FD) is caused by mutations in the GLA gene that encodes lysosomal α-galactosidase-A (α-gal-A). A number of pathogenic mechanisms have been proposed and these include loss of mitochondrial respiratory chain activity. For FD, gene therapy is beginning to be applied as a treatment. In view of the loss of mitochondrial function reported in FD, we have considered here the impact of loss of mitochondrial respiratory chain activity on the ability of a GLA lentiviral vector to increase cellular α-gal-A activity and participate in cross correction. Jurkat cells were used in this study and were exposed to increasing viral copies. Intracellular and extracellular enzyme activities were then determined; this in the presence or absence of the mitochondrial complex I inhibitor, rotenone. The ability of cells to take up released enzyme was also evaluated. Increasing transgene copies was associated with increasing intracellular α-gal-A activity but this was associated with an increase in Km. Release of enzyme and cellular uptake was also demonstrated. However, in the presence of rotenone, enzyme release was inhibited by 37%. Excessive enzyme generation may result in a protein with inferior kinetic properties and a background of compromised mitochondrial function may impair the cross correction process.
Human Circulating miRNAs Real-time qRT-PCR-based Analysis: An Overview of Endogenous Reference Genes Used for Data Normalization
Authors: Donati Simone , Ciuffi Simone , Brandi Maria L.
Published: September 2019

miRNAs are small non-coding RNAs of about 18–25 nucleotides that negatively regulate gene expression at the post-transcriptional level. It was reported that a deregulation of their expression patterns correlates to the onset and progression of various diseases. Recently, these molecules have been identified in a great plethora of biological fluids, and have also been proposed as potential diagnostic and prognostic biomarkers. Actually, real time quantitative polymerase chain reaction is the most widely used approach for circulating miRNAs (c-miRNAs) expression profiling. Nevertheless, the debate on the choice of the most suitable endogenous reference genes for c-miRNAs expression levels normalization is still open. In this regard, numerous research groups are focusing their efforts upon identifying specific, highly stable, endogenous c-mRNAs. The aim of this review is to provide an overview on the reference genes currently used in the study of various pathologies, offering to researchers the opportunity to select the appropriate molecules for c-miRNA levels normalization, when their choosing is based upon literature data.
Growth Hormone Secretagogues and the Regulation of Calcium Signaling in Muscle
Authors: Bresciani Elena , Rizzi Laura , Coco Silvia , Molteni Laura , Meanti Ramona , Locatelli Vittorio , Torsello Antonio
Published: September 2019

Growth hormone secretagogues (GHS) are a family of synthetic molecules, first discovered in the late 1970s for their ability to stimulate growth hormone (GH) release. Many effects of GHS are mediated by binding to GHS-R1a, the receptor for the endogenous hormone ghrelin, a 28-amino acid peptide isolated from the stomach. Besides endocrine functions, both ghrelin and GHS are endowed with some relevant extraendocrine properties, including stimulation of food intake, anticonvulsant and anti-inflammatory effects, and protection of muscle tissue in different pathological conditions. In particular, ghrelin and GHS inhibit cardiomyocyte and endothelial cell apoptosis and improve cardiac left ventricular function during ischemia–reperfusion injury. Moreover, in a model of cisplatin-induced cachexia, GHS protect skeletal muscle from mitochondrial damage and improve lean mass recovery. Most of these effects are mediated by GHS ability to preserve intracellular Ca2+ homeostasis. In this review, we address the muscle-specific protective effects of GHS mediated by Ca2+ regulation, but also highlight recent findings of their therapeutic potential in pathological conditions characterized by skeletal or cardiac muscle impairment.
Improvement of Epitope Prediction Using Peptide Sequence Descriptors and Machine Learning
Authors: Munteanu Cristian R. , Gestal Marcos , Martínez-Acevedo Yunuen G. , Pedreira Nieves , Pazos Alejandro , Dorado Julián
Published: September 2019

In this work, we improved a previous model used for the prediction of proteomes as new B-cell epitopes in vaccine design. The predicted epitope activity of a queried peptide is based on its sequence, a known reference epitope sequence under specific experimental conditions. The peptide sequences were transformed into molecular descriptors of sequence recurrence networks and were mixed under experimental conditions. The new models were generated using 709,100 instances of pair descriptors for query and reference peptide sequences. Using perturbations of the initial descriptors under sequence or assay conditions, 10 transformed features were used as inputs for seven Machine Learning methods. The best model was obtained with random forest classifiers with an Area Under the Receiver Operating Characteristics (AUROC) of 0.981 ± 0.0005 for the external validation series (five-fold cross-validation). The database included information about 83,683 peptides sequences, 1448 epitope organisms, 323 host organisms, 15 types of in vivo processes, 28 experimental techniques, and 505 adjuvant additives. The current model could improve the in silico predictions of epitopes for vaccine design. The script and results are available as a free repository.
Biomaterial-Based Approaches for Regeneration of Periodontal Ligament and Cementum Using 3D Platforms
Authors: Park Chan Ho
Published: September 2019

Currently, various tissue engineering strategies have been developed for multiple tissue regeneration and integrative structure formations as well as single tissue formation in musculoskeletal complexes. In particular, the regeneration of periodontal tissues or tooth-supportive structures is still challenging to spatiotemporally compartmentalize PCL (poly-ε-caprolactone)-cementum constructs with micron-scaled interfaces, integrative tissue (or cementum) formations with optimal dimensions along the tooth-root surfaces, and specific orientations of engineered periodontal ligaments (PDLs). Here, we discuss current advanced approaches to spatiotemporally control PDL orientations with specific angulations and to regenerate cementum layers on the tooth-root surfaces with Sharpey’s fiber anchorages for state-of-the-art periodontal tissue engineering.
Proteomics Approach for the Discovery of Rheumatoid Arthritis Biomarkers Using Mass Spectrometry
Authors: Mun Sora , Lee Jiyeong , Park Arum , Kim Hyo-Jin , Lee Yoo-Jin , Son Hyunsong , Shin Miji , Lim Mi-Kyoung , Kang Hee-Gyoo
Published: September 2019

Rheumatoid arthritis is an autoimmune disease that causes serious functional loss in patients. Early and accurate diagnosis of rheumatoid arthritis may attenuate its severity. Despite a diagnosis guideline in the 2010 American College of Rheumatology (ACR)/European League Against Rheumatism (EULAR) classification criteria for rheumatoid arthritis, the practical difficulties in its diagnosis highlight the need of developing new methods for diagnosing rheumatoid arthritis. The current study aimed to identify rheumatoid arthritis diagnostic biomarkers by using a proteomics approach. Serum protein profiling was conducted using mass spectrometry, and five distinguishable biomarkers were identified therefrom. In the validation study, the five biomarkers were quantitatively verified by multiple reaction monitoring (MRM) analysis. Two proteins, namely serum amyloid A4 and vitamin D binding protein, showed high performance in distinguishing patients with rheumatoid arthritis from healthy controls. Logistic analysis was conducted to evaluate how accurately the two biomarkers distinguish patients with rheumatoid arthritis from healthy controls. The classification accuracy was 86.0% and 81.4% in patients with rheumatoid arthritis and in healthy controls, respectively. Serum amyloid A4 and vitamin D binding protein could be potential biomarkers related to the inflammatory response and joint destruction that accompany rheumatoid arthritis.
Dual Specificity Phosphatases: From Molecular Mechanisms to Biological Function
Authors: Pulido Rafael , Lang Roland
Published: September 2019

Dual specificity phosphatases (DUSPs) constitute a heterogeneous group of enzymes, relevant in human disease, which belong to the class I Cys-based group of protein tyrosine phosphatase (PTP) gene superfamily [...]
P1G10, the Proteolytic Fraction from Vasconcellea cundinamarcensis, Stimulates Tissue Repair after Acute Exposure to Ultraviolet B Radiation
Authors: Freitas Kátia M. , Silva Ana C. Araújo e , Veloso Emerson S. , Ferreira Ênio , Barcelos Lucíola S. , Caliari Marcelo V. , Salas Carlos E. , Lopes Miriam T. P.
Published: September 2019

Background: P1G10 is a cysteine proteolytic fraction from Vasconcellea cundinamarcensis latex, obtained by chromatographic separation on Sephadex-G10 and ultrafiltration. This fraction enhances healing in different models of skin lesions, and displays a protective/healing effect against gastric ulcers, where it was suggested an antioxidant role. Methods: We evaluated here the effect of topical treatment with P1G10, in mice lesions induced by UVB. Results: After single exposure to 2.4 J cm−2 UVB, P1G10 reduced erythema, increased cellularity of hypodermis, enhanced MPO activity and IL1β, and inhibited COX2 levels. These results point to an anti-inflammatory effect by P1G10. This fraction displayed antioxidant activity by reversing the depletion of glutathione (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and reducing the catalase activity increased by UVB. These changes may be related to a reduction in MDA observed in groups treated with P1G10. P1G10 also inhibited MMP-9, caspase-3 and pkat while increasing p53 levels.
Multiple Mating in the Citrophilous Mealybug Pseudococcus calceolariae: Implications for Mating Disruption
Authors: Ricciardi Renato , Lucchi Andrea , Benelli Giovanni , Suckling David Maxwell
Published: September 2019

The citrophilous mealybug Pseudococcus calceolariae (Maskell) (Hemiptera, Pseudococcidae) is a primary pest of various crops, including grapevines. The use of insecticides against this species is difficult in most cases because its life cycle includes an extended duration of eggs, juveniles, and adults under the bark and on the roots. Pheromone-based control strategies can present new eco-friendly opportunities to manage this species, as in the case of Planococcus ficus (Signoret) and Planococcus citri (Risso). With this aim it is critical to understand behavioral aspects that may influence pheromone-based control strategies. Herein, the capability of males to fertilize multiple females was investigated, trying to understand whether this behavior could negatively impact the efficacy of mass trapping, mating disruption, or the lure and kill technique. Results showed that a P. calceolariae male can successfully mate and fertilize up to 13 females. The copulation time in subsequent mating events and the time between copulations did not change over time but the number of matings per day significantly decreased. In a further experiment, we investigated the mate location strategy of P. calceolariae males, testing the attractiveness of different loadings of sex pheromone on males in a flight tunnel. Males constantly exposed to 16 rubber septa loaded with the sex pheromone showed a significant decrease in female detection at 1 and 30 μg loadings (0.18 and 0.74 visits per female for each visit per septum, respectively), whereas in the control about 9.2-fold more of the released males successfully detected the female in the center of the array of 16 septa without pheromone. Male location of females in the control (45%) was significantly higher than in the arrays with surrounding pheromone (5% and 20% at 1 and 30 μg loadings, respectively). Mating only occurred in the control arrays (45%). This study represents a useful first step to developing pheromone-based strategies for the control of citrophilous mealybugs.
Choroidal Patterns in Stargardt Disease: Correlations with Visual Acuity and Disease Progression
Authors: Arrigo Alessandro , Grazioli Alessio , Romano Francesco , Aragona Emanuela , Bordato Alessandro , di Nunzio Carlo , Sperti Andrea , Bandello Francesco , Battaglia Parodi Maurizio
Published: September 2019

Background: To identify different choroidal patterns in Stargardt disease (STGD) and to assess their clinical correlates. Methods: 100 STGD eyes (29 males; mean age 42.6 ± 16.5 years) and 100 control eyes (29 males; mean age 43.2 ± 8.5 years) were included. Optical coherence tomography (OCT) and OCT angiography (OCTA) images were obtained. Four different choroidal patterns, quantitative OCT and OCTA parameters were assessed and statistically analyzed. The main outcome was the correlation between each choroidal pattern and anatomical and functional retinal status. Furthermore, we assessed structural and best corrected visual acuity (BCVA) changes of each STGD subgroup after one-year. Results: Mean BCVA was 0.63 ± 0.44 LogMAR for STGD patients and 0.0 ± 0.0 LogMAR for controls (p < 0.01). All quantitative parameters appeared deteriorated in STGD compared to controls (p < 0.01). Choroidal patterns were distributed as follows: Pattern 1 (normal appearing choroid) (15%), Pattern 2 (reduced Sattler or Haller layer) (29%), Pattern 3 (reduced Sattler and Haller layers) (26%), Pattern 4 (Pattern 3 + choroidal caverns) (30%). More advanced patterns significantly correlated with a more severe loss of retinal structural integrity. Furthermore, only Pattern 3 and Pattern 4 showed remarkable signs of progression after one year. Conclusions: Choroidal patterns were related with retinal structural status and BCVA loss, and with different disease progression.
Glucagon-Like Peptide-1 Receptor Agonists in Patients with Type 2 Diabetes: Prescription According to Reimbursement Constraints and Guideline Recommendations in Catalonia
Authors: Franch-Nadal Josep , Mata-Cases Manel , Ortega Emilio , Real Jordi , Gratacòs Mònica , Vlacho Bogdan , Vallés Joan Antoni , Mauricio Dídac
Published: September 2019

To assess the clinical characteristics, the prescription pattern of GLP-1 receptor agonists (GLP-1RA) users, and HbA1c and weight change, we retrospectively assessed patients with type 2 diabetes by initiating GLP-1RA as an add-on to the standard of care in Catalonia. The mean change from the baseline in glycated hemoglobin (HbA1c) and weight at 6 and 12 months of therapy was calculated, and we assessed the predictors of the HbA1c reduction of ≥1% and/or the weight reduction of ≥3% as recommended by the Catalan Health Service. In 2854 patients who initiated a GLP-1RA during 2014 and 2015, the overall mean HbA1c values were reduced from the baseline by −0.84% (SD = 1.66) (−9.2 mmol/mol) and lost on average 2.73 kg (SD = 6.2). About 44% percent of patients decreased their HbA1c by ≥1%; 44% decreased their weight by ≥3%; and only 22% met both of them together. The odds of achieving a reduction of ≥1% in initial HbA1c were two-fold higher for patients with higher baseline levels, and the likelihood of a reduction of ≥3% in the initial weight was associated with a higher BMI at the baseline, but they were independent of each other. The composite outcome (target 1% HbA1c reduction and 3% weight loss) to evaluate both the GLP-1RA clinical benefit and treatment withdrawal should be judged from a patient-centered approach.
Development and Mechanical Properties of Basalt Fiber-Reinforced Acrylonitrile Butadiene Styrene for In-Space Manufacturing Applications
Authors: Coughlin Natalie , Drake Bradley , Fjerstad Mikala , Schuster Easton , Waege Tyler , Weerakkody Adrian , Letcher Todd
Published: September 2019

A new basalt fiber reinforced acrylonitrile butadiene styrene (ABS) filament has been developed for fused filament fabrication (FFF, 3D printing) to be used in Mars habitat construction. Building habitats on Mars will be expensive, especially if all material must be shipped from earth. However, if some materials can be used from Mars, costs will dramatically decrease. Basalt is easily mined from the surface of Mars. This study details the production process of the material, experimental results from mechanical testing, and preliminary X-ray shielding characteristics. The addition of chopped 3 mm basalt fibers to standard FFF material, ABS, increased strength and stiffness of the composite material. By adding 25% (by weight) basalt fiber to ABS, tensile strength improved nearly 40% by increasing from 36.55 MPa to 50.58 MPa, while Modulus of Elasticity increased about 120% from 2.15 GPa to 4.79 GPa. Flexural strength increased by about 20% from 56.94 MPa to 68.51 MPa, while Flexural Modulus increased by about 70% from 1.81 GPa to 3.05 GPa. While compression results did not see much strength improvements, the addition of fibers also did not decrease compressive strength. This is important when considering that basalt fibers provide radiation shielding and the cost of adding basalt fibers to construction materials on Mars will be negligible compared to the cost of shipping other materials from earth. In preliminary digital radiography testing, it was shown that 77% of X-rays were shielded with 25% basalt fiber added (as compared to neat ABS). In small-scale 3D printing applications, the 25% fiber ratio seems to be the highest ratio that provides reliable FFF printing.
Comparative Characteristics of Ductile Iron and Austempered Ductile Iron Modeled by Neural Network
Authors: Savkovic Borislav , Kovac Pavel , Dudic Branislav , Gregus Michal , Rodic Dragan , Strbac Branko , Ducic Nedeljko
Published: September 2019

Experimental research of cutting force components during dry face milling operations are presented in the paper. The study was provided when milling of ductile cast iron alloyed with copper and its austempered ductile iron after the proper austempering process. In the study, virtual instrumentation designed for cutting forces components monitoring was used. During the research, orthogonal cutting forces components versus time were monitored and relationship of cutting forces components versus speed, feed and depth of cut were determined by artificial neural network and response surface methodology. An analysis was made regarding the consistency of the measured cutting forces and the values obtained from the model supported by an artificial neural network for the investigated interval of the cutting regime. Based on the results, an analysis of the feasibility of the application of austempered ductile iron in the industrial sector with the aspect of machinability as well as the application of the models based on artificial intelligence, was given. At the end of the presentation, the influence of the aforementioned cutting regimes on cutting force components is presented as well.
NiCo2S4 Nanotrees Directly Grown on the Nickel NP-Doped Reduced Graphene Oxides for Efficient Supercapacitors
Authors: Jang Wooree , Choi Won San , Lee Youn-Sik , Koo Hye Young
Published: September 2019

In this work, we report a feasible fabrication of NiCo2S4 nanotree-like structures grown from the Ni nanoparticle (NP)-doped reduced graphene oxides (Ni-rGO) by a simple hydrothermal method. It is found that the presence of Ni NPs on the surface of the rGOs initiates growth of the NiCo2S4 nanotree flocks with enhanced interfacial compatibility, providing excellent cyclic stability and rate performance. The resulting NiCo2S4/Ni-rGO nanocomposites exhibit a superior rate performance, demonstrating 91.6% capacity retention even after 10,000 cycles of charge/discharge tests.
In Situ Synthesis of Silicon–Carbon Composites and Application as Lithium-Ion Battery Anode Materials
Authors: Kim Dae-Yeong , Kim Han-Vin , Kang Jun
Published: September 2019

Silicon can be used in a variety of applications. Particularly, silicon particles are attracting increased attention as energy storage materials for lithium-ion batteries. However, silicon has a limited cycling performance owing to its peeling from the current collector and the volume expansion that occurs during alloying with lithium in the charging process. Significant contributors to this problem are the even distribution of silicon nanoparticles within the carbon matrix and their deep placement in the internal structure. In this study, we synthesized silicon nanoparticles and carbon materials via a bottom-up approach using a new method called plasma in solution. Silicon nanoparticles and the carbon matrix were synthesized in a structure similar to carbon black. It was confirmed that the silicon particles were evenly distributed in the carbon matrix. In addition, the evaluation of the electrochemical performance of the silicon–carbon matrix (Si–C) composite material showed that it exhibited stable cycling performance with high reversible capacity.
Constitutive Models for the Prediction of the Hot Deformation Behavior of the 10%Cr Steel Alloy
Authors: Shokry Abdallah , Gowid Samer , Kharmanda Ghias , Mahdi Elsadig
Published: September 2019

The aim of this paper is to establish a reliable model that provides the best fit to the specific behavior of the flow stresses of the 10%Cr steel alloy at the time of hot deformation. Modified Johnson–Cook and strain-compensated Arrhenius-type (phenomenological models), in addition to two Artificial Neural Network (ANN) models were established with the view toward investigating their stress prediction performances. The ANN models were trained using Scaled Conjugate Gradient (SCG) and Levenberg–Marquardt (LM) algorithms. The prediction accuracy of the established models was evaluated using the following well-known statistical parameters: (a) correlation coefficient (R), (b) Average Absolute Relative Error (AARE), (c) Root Mean Squared Error (RMSE), and Relative Error (RE). The results showed that both of the modified Johnson–Cook and strain-compensated Arrhenius models could not competently predict the flow behavior. On the contrary, the results indicated that the two proposed ANN models precisely predicted the flow stress values and that the LM-trained ANN provided a superior performance over the SCG-trained model, as it yielded an RMSE of as low as 0.441 MPa.
The Importance of DS-14 and HADS Questionnaires in Quantifying Psychological Stress in Type 2 Diabetes Mellitus
Authors: Pah Ana-Maria , Bucuras Petru , Buleu Florina , Tudor Anca , Iurciuc Stela , Velimirovici Dana , Streian Caius Glad , Badalica-Petrescu Marius , Christodorescu Ruxandra , Dragan Simona
Published: September 2019

Background and Objectives: The comorbid association between type 2 diabetes mellitus (T2DM) and a psychological profile characterized by depression and/or anxiety has been reported to increase the risk of coronary heart disease (CAD), the most striking macrovascular complication of diabetes. The purpose of the present study was to quantify anxiety, depression and the presence of type D personality, and to correlate the scores obtained with cardiovascular risk factors and disease severity in diabetic patients. Materials and methods: The retrospective study included 169 clinically stable diabetic patients divided into two groups: group 1 without macrovascular complications (n = 107) and group 2 with CAD, stroke and/or peripheral vascular disease (n = 62). A biochemical analysis and an assessment of psychic stress by applying the Hospital Anxiety and Depression Scale (HADS)and the Type D scale (DS-14) to determine anxiety, depression and D personality scores were done in all patients. Statistical analysis was made using SPSSv17 and Microsoft Excel, non-parametric Kruskal–Wallis and Mann–Whitney tests. Results: Following application of the HAD questionnaire for the entire group (n = 169), anxiety was present in 105 patients (62.2%), and depression in 96 patients (56.8%). Group 2 showed significantly higher anxiety scores compared to group 1 (p = 0.014), while depression scores were not significantly different. Per entire group, analysis of DS-14 scores revealed social inhibition (SI) present in 56 patients (33%) and negative affectivity (NA) in 105 patients (62%). TheDS-14 SI score was significantly higher in group 2 compared to group 1 (p = 0.036). Type D personality, resulting from scores above 10 in both DS-14 parameter categories, was present in 51 patients of the study group (30%). There was a direct and significant correlation (r = 0.133, p = 0.025) between the Hospital Anxiety and Depression Scale-Anxiety (HAD-A) score and the LDL-c values. Conclusions: The results of this study demonstrated that more than a half of patients with diabetes had anxiety and/or depression and one third had Type D personality, sustaining that monitoring of emotional state and depression should be included in the therapeutic plan of these patients. New treatment strategies are needed to improve the well-being of diabetic patients with psychological comorbidities.
Comparison of Differential Flavor Metabolites in Meat of Lubei White Goat, Jining Gray Goat and Boer Goat
Authors: Wang Weiting , Sun Bei , Hu Peng , Zhou Meng , Sun Sujun , Du Pengfei , Ru Yi , Suvorov Alexander , Li Yongsheng , Liu Yaobo , Wang Shoujing
Published: September 2019

Flavor is one of the most important sensory characteristics of meat. The development of taste and aroma can be attributed to thousands of flavor molecules and precursors that are present in meat tissues. As a result, the identification of these flavor compounds and an improved understanding of their roles are necessary for improving the sensory quality and customer appeal of meat products. In the current study, we compared the metabolic profiles of meat specimens from the Lubei white goats (LBB), Boer goats (BE) and Jining grey goats (JNQ) by untargeted liquid chromatography-mass spectrometry. Our metabolomic data revealed that the three types of goat meat showed significantly different profiles of fatty acids, aldehydes, ketones, lactones, alkaloids, flavonoids, phenolics and drug residues, which could underpin the nuances of their flavors. Taken together, our results provided insights into the molecular basis for sensory variations between different goat meat products.
Oxylipin Profiling of Alzheimer’s Disease in Nondiabetic and Type 2 Diabetic Elderly
Authors: Morris Jill K. , Piccolo Brian D. , John Casey S. , Green Zachary D. , Thyfault John P. , Adams Sean H.
Published: September 2019

Oxygenated lipids, called “oxylipins,” serve a variety of important signaling roles within the cell. Oxylipins have been linked to inflammation and vascular function, and blood patterns have been shown to differ in type 2 diabetes (T2D). Because these factors (inflammation, vascular function, diabetes) are also associated with Alzheimer’s disease (AD) risk, we set out to characterize the serum oxylipin profile in elderly and AD subjects to understand if there are shared patterns between AD and T2D. We obtained serum from 126 well-characterized, overnight-fasted elderly individuals who underwent a stringent cognitive evaluation and were determined to be cognitively healthy or AD. Because the oxylipin profile may also be influenced by T2D, we assessed nondiabetic and T2D subjects separately. Within nondiabetic individuals, cognitively healthy subjects had higher levels of the nitrolipid 10-nitrooleate (16.8% higher) compared to AD subjects. AD subjects had higher levels of all four dihydroxyeicosatrienoic acid (DiHETrE) species: 14,15-DiHETrE (18% higher), 11,12 DiHETrE (18% higher), 8,9-DiHETrE (23% higher), and 5,6-DiHETrE (15% higher). Within T2D participants, we observed elevations in 14,15-dihydroxyeicosa-5,8,11-trienoic acid (14,15-DiHETE; 66% higher), 17,18-dihydroxyeicosa-5,8,11,14-tetraenoic acid (17,18-DiHETE; 29% higher) and 17-hydroxy-4,7,10,13,15,19-docosahexaenoic acid (17-HDoHE; 105% higher) and summed fatty acid diols (85% higher) in subjects with AD compared to cognitively healthy elderly, with no differences in the DiHETrE species between groups. Although these effects were no longer significant following stringent adjustment for multiple comparisons, the consistent effects on groups of molecules with similar physiological roles, as well as clear differences in the AD-related profiles within nondiabetic and T2D individuals, warrant further research into these molecules in the context of AD.
Multivariate Analysis Based on Geochemical, Isotopic, and Mineralogical Compositions of Uranium-Rich Samples
Authors: Corcoran Loretta , Simonetti Antonio , Spano Tyler , Lewis Stefanie , Dorais Corinne , Simonetti Stefanie , Burns Peter
Published: September 2019

The chemical and isotopic (U, Pb, Sr) signatures for a suite (n = 23) of pristine (>80 wt. % UO2) and altered uraninite samples (>70–80 wt. % UO2) from various locations worldwide have been determined for the purpose of identifying potential fingerprints for nuclear forensic analysis. The characterization of the uraninite samples included determination of major, minor and trace element contents, Sr, Pb, and U isotopic compositions, and secondary mineral assemblages. Due to the multivariate approach adopted in this study, principal component analysis (PCA) has been employed to allow the direct comparison of multiple variable types. The PCA results indicate that the geological origin (sandstone, metamorphite, intrusive, granite and unconformity) of pristine uraninite can be readily identified utilizing various combinations of major and/or trace element concentrations with isotopic compositions.
Geochemistry, Zircon U-Pb Geochronology and Hf-O Isotopes of the Banzhusi Granite Porphyry from the Xiong’ershan Area, East Qinling Orogen, China: Implications for Petrogenesis and Geodynamics
Authors: Wang Bin , Hu Xinkai , Tang Li , Li Jingchao
Published: September 2019

The Banzhusi granite porphyry is located in the Xiong’ershan area, East Qinling orogenic belt (EQOB). This study presents an integrated whole-rock geochemistry and zircon U-Pb-Hf-O isotope analysis of the Banzhusi granite porphyry. These rocks have metaluminous, high-K alkali-calcic and shoshonitic features and show significant enrichment in light rare earth elements (LREEs) over heavy rare earth elements (HREEs) with negative Eu anomalies. These samples are also greatly enriched in Rb, Ba, K, Pb, Th and U and depleted in Nb, Ta, P and Ti, and they mostly overlap the ranges of the Taihua Group tonalite–trondhjemite–granodiorite (TTG) gneiss. Magmatic zircons from three samples of the Banzhusi granite porphyry yield U-Pb ages of 125.1 ± 0.97 Ma, 128.1 ± 1.2 Ma and 128.2 ± 1.3 Ma. The Hf-O isotope features of zircons from the three samples are very similar (δ18Ozircon = 4.84‰ to 6.51‰, εHf(t) = −26.9 to −14.4). The co-variations of geochemical and isotopic data in these granite porphyries imply that the Banzhusi granite porphyry resulted from the mixing of the partially melted Taihua Group and mantle-derived material in a post-collisional setting from 128–125 Ma.