bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison between Novel Anatomical Locking Guide Plate and Conventional Locking Plate for Acetabular Fractures: A Finite Element Analysis
Authors: Liu Xiao , Gao Jianpeng , Wu Xiaoyong , Deng Junhao , Li Zijian , Li Ran , Zhang Licheng , Liu Jianheng , Li Ming
Published: October 2023

The treatment of complex acetabular fractures remains a complicated clinical challenge. Our self-designed novel anatomical locking guide plate (NALGP) has previously shown promising potential in T-shaped acetabular fractures (TAF), but a direct comparison with conventional fixations is yet to be made. The TAF model was established based on a volunteer’s computer tomography data and then fixed with double column locking plates (DLP), a posterior column locking plate with anterior column screws (LPACS), and our NALGP. Forces of 200 N, 400 N, and 600 N were then loaded on the model vertically downward, respectively. The stress distribution and peaks and maximum displacements at three sites were assessed. We found that the stress area of all three plates was mainly concentrated around the fracture line, while only the matching screws of the NALGP showed no obvious stress concentration points. In addition, the NALGP and DLP showed significantly less fracture fragment displacement than the LPACS at the three main fracture sites. The NALGP was found to have less displacement than DLP at the posterior column and ischiopubic branch sites, especially under the higher loading forces of 400 N and 600 N. The fixation stability of the NALGP for TAF was similar to that of DLP but better than that of LPACS. Moreover, the NALGP and its matching screws have a more reasonable stress distribution under different loads of force and the same strength as the LPACS.
Risk of SARS-CoV-2 Reinfection 3 Years after the Start of the Pandemic: A Population-Level Observational Study
Authors: Acuti Martellucci Cecilia , Flacco Maria Elena , Soldato Graziella , Di Martino Giuseppe , Carota Roberto , Rosso Annalisa , De Benedictis Marco , Di Marco Graziano , Di Luzio Rossano , Lisbona Francesco , Caponetti Antonio , Manzoli Lamberto
Published: October 2023

The risk of SARS-CoV-2 reinfections changes as new variants emerge, but the follow-up time for most of the available evidence is shorter than two years. This study evaluated SARS-CoV-2 reinfection rates in the total population of an Italian province up to three years since the pandemic’s start. This retrospective cohort study used official National Healthcare System data on SARS-CoV-2 testing and vaccinations, demographics, and hospitalizations in the Province of Pescara, Italy, from 2 March 2020 to 31 December 2022. A total of 6541 (5.4%) reinfections and 33 severe and 18 lethal COVID-19 cases were recorded among the 121,412 subjects who recovered from a primary infection. There were no severe events following reinfection in the young population, whereas 1.1% of reinfected elderly died. A significantly higher reinfection risk was observed among females; unvaccinated individuals; adults (30–59 y); and subjects with hypertension, COPD, and kidney disease. Up to three years after a primary SARS-CoV-2 infection, the majority of the population did not experience a reinfection. The risk of severe COVID-19 following a reinfection was very low for young and adult individuals but still high for the elderly. The subjects with hybrid immunity showed a lower reinfection risk than the unvaccinated.
A Study on Surface Hardening and Wear Resistance of AISI 52100 Steel by Ultrasonic Nanocrystal Surface Modification and Electrolytic Plasma Surface Modification Technologies
Authors: Magazov Nurtoleu , Satbaeva Zarina , Rakhadilov Bauyrzhan , Amanov Auezhan
Published: October 2023

In this study, a surface hardening of AISI 52100 bearing steel was performed by ultrasonic nanocrystal surface modification (UNSM), and electrolytic-plasma thermo-cyclic surface modification (EPSM), and their effects on the wear resistance were investigated. To evaluate the impact of these treatments on the wear resistance, the friction tests under dry conditions were conducted using a ball-on-disk tribometer in accordance with ASTM G99. The microstructure of the samples before and after treatment was characterized by scanning electron microscopy. The micro-hardness with respect to the depth from the top surface was measured using a Vickers micro-hardness tester. Microstructural observations showed that EPSM treatment led to the formation of residual austenite in the surface layer, while UNSM treatment led to the formation of a surface severe plastic deformation layer on the surface of the samples. The increase in the micro-hardness of the treated layer was confirmed after UNSM at room temperature and after EPSM at different cycles. The highest increase in wear resistance was observed for the specimen treated by UNSM treatment at 700 °C and five cycles of EPSM treatment. In addition, the wear volume, which has correlation with the friction coefficient and hardness, was determined.
Selective Laser Spectroscopy of the Bixbyite-Type Yttrium Scandate Doped by Rare-Earth Ions
Authors: Dobretsova Elena , Alimov Olimkhon , Rusanov Sergey , Kashin Vitaly , Tsvetkov Vladimir
Published: October 2023

Yttrium scandate crystals doped by Nd3+ and Tm3+ ions have been successfully grown in the form of fibers using the laser-heated pedestal growth (LHPG) technique. The selective laser spectroscopy methods have identified and distinguished three distinct types of optically active centers associated with Nd3+ and Tm3+ ions. The substitution of Y3+ and Sc3+ for rare-earth ions in the C2 structural site leads to the formation of two distinct basic long-time centers. In Nd3+:YScO3, another type of center (a short-lifetime one) is formed known as the Nd3+–Nd3+ aggregate pair. This center arises from the substitution of Y3+ or Sc3+ for Nd3+ cation in the adjacent MO6 polyhedra that share an edge. In Tm3+:YScO3, the third optical center is formed as a result of the substitution of Y3+ or Sc3+ for Tm3+ in the MO6 octahedra with the C3i site symmetry. The fluorescence decay lifetimes of Nd3+ and Tm3+ ions in the YScO3 crystal structure have been accurately measured and estimated. A Stark level diagram illustrating the splitting of 4F3/2, 4I11/2, and 4I9/2 multiplets of Nd3+ ions has been constructed to show features of the active optical centers with the C2 site symmetry.
Wood Esterification by Fatty Acids Using Trifluoroacetic Anhydride as an Impelling Agent and Its Application for the Synthesis of a New Bioplastic
Authors: Sejati Prabu Satria , Obounou Akong Firmin , Fradet Frédéric , Gérardin Philippe
Published: October 2023

Fatty acids (FA) and their derivatives with long alkyl chain structures are good candidates for wood esterification to confer thermoplastic properties to wood. Nevertheless, they do not react easily with hydroxyl groups of wood. In this study, we investigated the reactivity of wood with various fatty acids of different chain lengths using trifluoroacetic anhydride (TFAA) as the impelling agent in various reaction conditions. Generally, the esterification of fatty acids without solvents resulted in higher Weight Percentage Gain (WPG) and ester content than the reaction in the presence of CH2Cl2. The esterification reaction could be performed effectively at room temperature, though an increased reaction temperature provoked degradation of the esterified wood. WPG of 67% was obtained for the C3 and 253% for the C16 alkyl chain analogs, respectively. Nevertheless, the ester content was fairly uniform, with values between 10.60 and 11.81 mmol ester/gram of wood for all chain lengths. A higher quantity of reagent led to higher ester content, which tended to stabilize after a ratio of 1:4 wood and TFAA/FA. The esterification reaction was performed rapidly, with an ester content between 7.65 and 9.94 mmol ester/gram of wood being achieved only after 15 min of reaction. Fourier transform infrared spectroscopy (FTIR) analysis was performed to confirm the drastic chemical changes of wood before and after esterification. Morphological observation by scanning electron microscope (SEM), softening measurement by thermomechanical analysis (TMA), and contact angle measurements demonstrated the possibility of esterified spruce wood being applied as a new bioplastic.
Judd–Ofelt Analysis and Spectroscopy Study of Tellurite Glasses Doped with Rare-Earth (Nd3+, Sm3+, Dy3+, and Er3+)
Authors: Boudchicha Naziha , Iezid Mostepha , Goumeidane Faycal , Legouera Messaoud , Prasad P. Syam , Rao P. Venkateswara
Published: October 2023

A series of glasses based on (80-y) TeO2-20 BiCl3-y RE2O3 (y = 0, 0.6 mol%; RE = Nd, Sm, Dy, and Er) were prepared. The thermal stability of the glass was determined by differential scanning calorimetry (DSC). The density and optical energy values of the prepared glass increased in the order of Sm2O3, Nd2O3, Dy2O3, and Er2O3. In addition, the glass doped with Er2O3 had the highest refractive index values compared to the other samples. Subsequently, Judd–Ofelt parameters (Ω2, Ω4, and Ω6) were obtained for the family of RE3+ trivalent rare-earth ions introduced as dopants in a tellurite glass. These parameters were calculated from the absorption spectra for each RE3+. The structures were studied by Raman spectroscopy deconvolution, which determined that TeO4, TeO3, TeO3+1, BiO6, and BiCl6 units had formed. In addition, the structural changes in the glass are related to the intensity ratio of TeO4/TeO3, depending on the type of rare-earth. For the optics and Judd–Ofelt parameters, the ray spectroscopy results of the prepared glass show that it is a good candidate for nonlinear optics fibers, a solid laser material.
Investigation of the Combined Influence of Temperature and Humidity on Fatigue Crack Growth Rate in Al6082 Alloy in a Coastal Environment
Authors: Alqahtani Ibrahim , Starr Andrew , Khan Muhammad
Published: October 2023

The fatigue crack growth rate (FCGR) of aluminium alloys under the combined influence of temperature and humidity remains a relatively unexplored area, receiving limited attention due to its intricate nature and challenges in predicting the combined impact of these factors. The challenge was to investigate and address the specific mechanisms and interactions between temperature and humidity, as in coastal environment conditions, on the FCGR of aluminium alloy. The present study conducts a comprehensive investigation into the combined influence of temperature and humidity on the FCGR of the Al6082 alloy. The fatigue pre-cracked compact tension specimens were corroded for 7 days and then subjected to various temperature and humidity conditions in a thermal chamber for 3 days to simulate coastal environments. The obtained data were analysed to determine the influence of temperature and humidity on the FCGR of the Al6082 alloy. An empirical model was also established to precisely predict fatigue life cycle values under these environmental conditions. The correlation between FCGR and fracture toughness models was also examined. The Al6082 alloy exhibits a 34% increase in the Paris constant C, indicating reduced FCGR resistance due to elevated temperature and humidity levels. At the same time, fatigue, corrosion, moisture-assisted crack propagation, and hydrogen embrittlement lead to a 27% decrease in threshold fracture toughness. The developed model exhibited accurate predictions for fatigue life cycles, and the correlation between fracture toughness and FCGR showed an error of less than 10%, indicating a strong relationship between these parameters.
Buckling Analysis of Thin-Walled Composite Structures with Rectangular Cross-Sections under Compressive Load
Authors: Rozylo Patryk , Rogala Michal , Pasnik Jakub
Published: October 2023

The purpose of this research was the analysis of the stability of compressed thin-walled composite columns with closed rectangular cross-sections, subjected to axial load. The test specimens (made of carbon–epoxy composite) were characterized by different lay-ups of the composite material. Experimental tests were carried out using a universal testing machine and other interdisciplinary testing techniques, such as an optical strain measurement system. Simultaneously with the experimental studies, numerical simulations were carried out using the finite element method. In the case of FEA simulations, original numerical models were derived. In the case of both experimental research and FEM simulations, an in-depth investigation of buckling states was carried out. The measurable effect of the research was to determine both the influence of the cross-sectional shape and the lay-up of the composite layers on the stability of the structure. The novelty of the present paper is the use of interdisciplinary research techniques in order to determine the critical state of compressed thin-walled composite structures with closed sections. An additional novelty is the object of study itself—that is, thin-walled composite columns with closed sections.
The Wear Behavior of Glass-Ceramic CAD/CAM Blocks against Bovine Enamel
Authors: Someya Tomoko , Kasahara Masaaki , Takemoto Shinji , Hattori Masayuki
Published: October 2023

The wear of enamel and crown restorative materials often occur by occlusion. The purpose of this study was to evaluate the wear volume between glass-ceramics used for CAD/CAM blocks (lithium disilicate: Initial LiSi block (LIS), IPS e.max CAD (IPS), zirconia-reinforced lithium silicate glass-ceramics: Celtra DUO (DUO), VITA Suprinity (VITS) and feldspar-based glass-ceramics: Vitablocs Mark II (MAK)) and bovine tooth enamel using a two-body wear test, the hardness, three-point bending strength, micro-structure and the element components of glass-ceramics. The data were analyzed using a one-way analysis of variance and Tukey’s multiple comparison test (α = 0.05). IPS and DUO with relatively large size crystal gain had significantly larger abrader wear volumes. Zirconia-reinforced lithium silicate glass-ceramics (DUO, VITS) caused significantly greater wear volume in antagonist enamel. MAK with scale-shape crystals grains produced distinct scratches after wear tests, both in the material itself and in the enamel. A strong correlation between the mechanical properties (hardness, three-point bending strength) and wear volume could not be confirmed. The type of glass-ceramic, size, and shape of the crystal grains affected the wear behavior of the glass-ceramics for CAD/CAM blocks. Therefore, dentists should consider that wear behavior varies with crystal structure, size, and shape in glass-ceramics for CAD/CAM blocks.
CHBS-Net: 3D Point Cloud Segmentation Network with Key Feature Guidance for Circular Hole Boundaries
Authors: Zhang Jiawei , Wang Xueqi , Li Yanzheng , Liu Yinhua
Published: October 2023

In laser scanning inspection systems for sheet metal parts, the rapid and accurate inspection of the high-precision holes is not only crucial but difficult. The accuracy of the circular holes, especially the locating datum holes on the parts, plays an important role in the assembly quality. However, accurately segmenting the circular hole boundary points required for circular hole fitting from large-scale scanning point cloud data remains one of the most difficult tasks for inspection accuracy improvement. To address this problem, a segmentation network called the circular hole boundary segmentation network (CHBS-Net) is proposed for boundary point cloud extraction. Firstly, an encoding–decoding–attention (EDA) fusion guidance mechanism is used to address the imbalance in data distribution due to the small proportion of boundary points in the overall point cloud. Secondly, a long short-term memory (LSTM) network parallel structure is used to capture the contour continuity and temporal relationships among boundary points. Finally, the interference of neighboring points and noise is reduced by extracting features in the multi-scale neighborhood. Experiments were performed using real cases from a sheet metal parts dataset to illustrate the procedures. The results showed that the proposed method achieves better performance than the benchmark state-of-the-art methods. The circular hole inspection accuracy is effectively improved by enhancing the segmentation accuracy of the scanning boundary points.
Boundedness and Compactness of Weighted Composition Operators from α-Bloch Spaces to Bers-Type Spaces on Generalized Hua Domains of the First Kind
Authors: Wang Jiaqi , Su Jianbing
Published: October 2023

We address weighted composition operators ψCϕ from α-Bloch spaces to Bers-type spaces of bounded holomorphic functions on Y, where Y is a generalized Hua domain of the first kind, and obtain some necessary and sufficient conditions for the boundedness and compactness of those operators.
Risk Factors and Predictive Model for Mortality of Hospitalized COVID-19 Elderly Patients from a Tertiary Care Hospital in Thailand
Authors: Chuansangeam Mallika , Srithan Bunyarat , Pattharanitima Pattharawin , Phadungsaksawasdi Pawit
Published: October 2023

Background: Early detection of elderly patients with COVID-19 who are at high risk of mortality is vital for appropriate clinical decisions. We aimed to evaluate the risk factors associated with all-cause in-hospital mortality among elderly patients with COVID-19. Methods: In this retrospective study, the medical records of elderly patients aged over 60 who were hospitalized with COVID-19 at Thammasat University Hospital from 1 July to 30 September 2021 were reviewed. Multivariate logistic regression was used to identify independent predictors of mortality. The sum of weighted integers was used as a total risk score for each patient. Results: In total, 138 medical records of patients were reviewed. Four identified variables based on the odds ratio (age, respiratory rate, glomerular filtration rate and history of stroke) were assigned a weighted integer and were developed to predict mortality risk in hospitalized elderly patients. The AUROC of the scoring system were 0.9415 (95% confidence interval, 0.9033–0.9716). The optimized scoring system was developed and a risk score over 213 was considered a cut-off point for high mortality risk. Conclusions: A simple predictive risk score provides an initial assessment of mortality risk at the time of admission with a high degree of accuracy among hospitalized elderly patients with COVID-19.
Study on the Effect of Preheating Temperatures on Melt Pool Stability in Inconel 718 Components Processed by Laser Powder Bed Fusion
Authors: Baldi Niccolò , Giorgetti Alessandro , Palladino Marco , Giovannetti Iacopo , Arcidiacono Gabriele , Citti Paolo
Published: October 2023

Laser Powder Bed Fusion (L-PBF) is one of the most widespread, versatile, and promising metal Additive Manufacturing (AM) techniques. L-PBF allows for the manufacturing of geometrically complex parts with good surface characteristics. In this process, in order to minimize the heat loss in the first layers of printing, the building platform is preheated to a temperature ranging between 80 and 250 °C. This aspect turns out to be very critical, and further investigation is needed for situations where the part to be printed is only a few layers high, as is the case in sensor printing. This work aims to investigate the melt pool stability under a variation in the preheating temperatures. We investigate the distance from the building platform, considering the number of layers printed. This is where the melt pool reaches its stability in terms of depth and width. This aspect turns out to be of remarkable importance for ensuring the structural integrity of parts with a few layers of height that are processed through L-PBF, such as sensors, which are proliferating in different industries. Thus, two case studies were carried out on IN718 superalloys at 40 and 60 microns of layer thickness and a preheating temperature of 170 °C on the machine. The results obtained show that after 1.2 mm of distance from the building platform, the melt pool reached its stability in terms of width and depth dimensions and consequently for the melting regime.
Influence of Technological Parameters on Chip Formation and Chip Control in Precision Hard Turning of Ti-6Al-4V
Authors: Abdelnasser Elshaimaa , El-Sanabary Samar , Nassef Ahmed , Barakat Azza , Elkaseer Ahmed
Published: October 2023

This article presents the results of an experimental investigation into the effect of process parameters in the precision hard turning of Ti-6Al-4V on chip morphology at both macro and micro levels. It also reports on the control of chip generation to improve chip evacuation and breakability at the macro level by varying the process parameters, namely, feed rate, cutting speed and depth of cut during turning tests. A scanning electron microscope (SEM) was used to examine the chips produced for a better understanding of chip curling mechanisms at the micro level. Surface roughness of the machined specimens was measured to assess the effect of chip evacuation on obtainable surface quality. From the results, it was found that the interaction of process parameters has a significant effect on the control of chip formation. In particular, the interaction of higher cutting speeds and greater depths of cut produced chip entanglement with the workpiece for all values of feed rates. Using relatively higher feed rates with a low depth of cut showed good results for chip breaking when machining at higher cutting speeds. Different chip curling mechanisms were identified from the SEM results. Chip side-curl formation showed different segmentation patterns with an approximately uniform chip thickness along the chip width, while chip up-curl occurred due to variations in chip thickness. Finally, it was found that the tangling of the chip with the workpiece has a significant effect on the final surface quality.
Experimental Study of the Surface Quality of Form-Cutting Tools Manufactured via Wire Electrical Discharge Machining Using Different Process Parameters
Authors: Alinaghizadeh Amir , Hadad Mohammadjafar , Azarhoushang Bahman
Published: October 2023

Form-cutting tools are an economical choice for turning parts with defined profiles in mass production. The effects of the form contour of these tools—produced by the wire electrical discharge machining (WEDM) process—on tool quality were investigated in this research. This study focuses on reducing the adverse effects of the recast layer induced by WEDM on form-cutting tools. The basic component types of profile forms in form-cutting tools can be summarized by a combination of four modes, i.e., concave and convex arcs and flat and oblique surfaces. Hence, sample cutting tools with three different radii of convex and concave arcs and a flat surface were produced. During the WEDM operation, one-pass mode was used for roughing, two passes for semi-finishing, and three passes for finishing. Furthermore, the difference between the percentage of oxygen and carbon elements on the recast layer in the two areas above the workpiece or wire entry point and the bottom area of the workpiece or wire exit point was investigated. Finally, the influences of the direction, size of the curvature, and the number of passes in the wire electric discharge process on the recast layer were analyzed. It was observed that the recast layer thickness could be reduced by increasing the number of WEDM process. Additionally, the uniformity of the cutting contour was superior in the entry region of the wire going into the workpiece compared with the exit region of the wire.
Arbuscular Mycorrhizal Fungi Associated with Maize (Zea mays L.) in the Formation and Stability of Aggregates in Two Types of Soil
Authors: Gómez-Leyva Juan Florencio , Segura-Castruita Miguel Angel , Hernández-Cuevas Laura Verónica , Íñiguez-Rivas Mayra
Published: October 2023

Knowledge of native Arbuscular Mycorrhizal Fungi (AMF) and their relationship with the edaphic characteristics where they live is important to establish the influence of allochthonous AMF, which were inoculated, on the development and stability of soil aggregates. The objectives of this research were to know the composition of native AMF species from two contrasting soils, and to establish the development and stability of aggregates in those soils with corn plants after inoculating them with allochthonous AMF. The experiment had three factors: Soil (two levels [S1 and S2]), HMA (three levels: without application [A0], with the application of Claroideoglomus claroideum [A1] and with the application of a consortium [A2]) and Fertilization (two levels (without fertilization [f0] and with fertilization [f1])). Twelve treatments were generated, with five replicates (60 experimental units [EU]). The EU consisted of a pot with a corn plant and the distribution was completely random. The results demonstrated that the Typic Ustifluvent presented nine species of native AMF, while the Typic Dystrustert had three; the native AMF in each soil influenced the activity of allochthonous AMF, such as their colonization and sporulation. Likewise, differences were found in the stability of macro-sized aggregates (0.5 to 2.0 mm).
Copper Isotopes and Constraints on the Ore Genesis Process of Cu-Co Ore Deposits at the Idaho Cobalt Belt, USA
Authors: Zaronikola Nina , Holley Elizabeth A. , Mathur Ryan , Pace Dan
Published: October 2023

Quantifying and identifying the introduction of metal in ore deposits that have experienced multiple overprinting hydrothermal events remains an elusive yet essential goal in metallogenic studies. Here, we constrain the origin of Co in the Idaho Cobalt Belt (ICB) that experienced two distinct metal-rich events that introduced Co and Cu. We performed a detailed petrographic study of sulfide ore at Iron Creek in the ICB, in concert with the quantification of trace metal element concentrations and copper isotope values to identify the introduction of Co in the system. The pyrite displays various degrees of alteration, with the highest Co concentrations (up to 6 wt.%) in less-altered pyrite grains (e.g., sharp edges, absence of altered boundaries and fissures) and highest δ65Cu isotope value. The most-altered pyrite grains (e.g., corroded grains, round and altered boundaries) have lower Co contents and lower δ65Cu isotope values that match the copper isotope values of the chalcopyrite. The least-altered pyrite shows a narrow δ65Cu range between −0.39‰ to −0.58‰. In contrast, the most-altered pyrite grains are isotopically depleted, showing a δ65Cu range from −1.35‰ to −0.90‰. Chalcopyrite shows a δ65Cu range between −1.07‰ and −0.77‰. We interpret, from the Cu isotope compositions and Co concentrations in pyrite, that the Co was originally introduced into the siliciclastic host rock package in a Mesoproterozoic SEDEX environment. The heavier Cu was then preferentially leached in a second event, resulting in isotopically lighter Cu in the altered pyrite. Remobilization of the SEDEX cobalt was likely associated with CO2-rich metamorphic fluids present in the region during the Mesoproterozoic East Kootenay orogeny, the late Mesoproterozoic Grenville orogeny, and the Late Jurassic to Late Cretaceous Cordilleran orogeny.
Impact of Drying Process on the Phenolic Profile and Antioxidant Capacity of Raw and Boiled Leaves and Inflorescences of Chenopodium berlandieri ssp. berlandieri
Authors: Vargas-Madriz Ángel Félix , Kuri-García Aarón , Luzardo-Ocampo Ivan , Vargas-Madriz Haidel , Pérez-Ramírez Iza Fernanda , Anaya-Loyola Miriam Aracely , Ferriz-Martínez Roberto Augusto , Roldán-Padrón Octavio , Hernández-Sandoval Luis , Guzmán-Maldonado Salvador Horacio , Chávez-Servín Jorge Luis
Published: October 2023

C. berlandieri ssp. berlandieri (C. berlandieri) is one of the most common members of the group of plants known as quelites, which are dark leafy greens widely consumed in Mexico. This study aimed to evaluate the impact of two drying procedures (oven drying and freeze-drying/lyophilization) on the polyphenolic composition, antioxidant capacity, and proximal chemical analysis of C. berlandieri leaves and inflorescences (raw or boiled). The results indicated that the raw freeze-dried samples had higher amounts (p < 0.05) of total phenolic compounds, total flavonoids, and antioxidant capacity, mainly in the inflorescence. The oven-dried samples showed an increased concentration of polyphenols after boiling, while the lyophilized samples showed a slightly decreased concentration. The drying process was observed to have little impact on the proximal chemical composition. Quantification by UPLC-DAD-ESI-QToF/MS identified up to 23 individual phenolic compounds, with freeze-dried samples showing higher amounts of individual compounds compared with oven-dried. Procyanidin B2 was found exclusively in the inflorescences. The inflorescences have a higher content of phenolic compounds and greater antioxidant capacity than the leaves. Regardless of the drying process, the leaves and inflorescences of C. berlandieri contain an interesting variety of phenolic compounds that may have beneficial effects on health.
Effective Reinforcement of Visible Light Photocatalytic and Gas Sensing Characteristics of Nanocrystalline TiO2: Gd-Based Nb and Mo Dopants
Authors: Alsulaim Ghayah M.
Published: October 2023

Efficient compositions for the selective detection of ethanol gas and the removal of organic contaminants were realized by codoping of (Gd, Nb) and (Gd, Mo) ions into TiO2. TiO2, Ti0.96Gd0.01Nb0.03O2, and Ti0.96Gd0.01Mo0.03O2 samples were prepared by a coprecipitation method. For all compositions, a crystalline anatase phase of TiO2 was detected. Compared to pure TiO2, the absorption edges of Ti0.96Gd0.01Nb0.03O2 and Ti0.96Gd0.01Mo0.03O2 samples were red-shifted, further broadening towards visible light. The morphological studies demonstrate that the grains of TiO2 were more refined after (Gd, Nb) and (Gd, Mo) codoping. The photocatalytic efficiency of the Ti0.96Gd0.01Mo0.03O2 catalyst for degrading 20 mg/L reactive yellow 145, brilliant green, and amoxicillin was 98, 95, and 93% in 90 min, respectively. The reusability experiments indicate that the Ti0.96Gd0.01Mo0.03O2 catalyst had high stability during reuse. The high photocatalytic activity of the Ti0.96Gd0.01Mo0.03O2 catalyst was correlated to the broad visible-light absorption and effective separation of electron–hole pairs by Gd3+ and Mo6+ cations. The gas sensing characteristic is reflected by the high sensitivity of the Ti0.96Gd0.01Nb0.03O2 sensor to ethanol gas in the presence of different gases at 275 °C. The obtained results indicated that the (Gd, Mo) mixture could more effectively induce the photocatalytic properties of TiO2 while (Gd, Nb) dopants were the best for reinforcing its sensing characteristics.
Analysis of Skin Regeneration and Barrier-Improvement Efficacy of Polydeoxyribonucleotide Isolated from Panax Ginseng (C.A. Mey.) Adventitious Root
Authors: Lee Kwang-Soo , Lee Soyeon , Wang Hyesoo , Lee Geonhee , Kim Seolyeong , Ryu Yang-Hwan , Chang Nicole Hyesoo , Kang Yong-Won
Published: October 2023

Polydeoxyribonucleotide (PDRN) has the ability to regenerate skin cells and improve the skin barrier and wound healing. This study investigated the possibility of replacing animal-derived PDRN with plant-derived PDRN. To test this, the adventitious roots of Korean ginseng (Panax ginseng C.A. Meyer), which is commonly used to treat various diseases, were suspension-cultivated through tissue culture; subsequently, PDRN was purified using microfluidization, an ultra-high-pressure physical grinding method. The results showed that purified Panax PDRN was effective in healing skin wounds and enhancing the skin barrier. Panax PDRN promoted the proliferation of keratinocytes and fibroblasts by increasing the expression of fibronectin, filaggrin, Ki-67, Bcl-2, inhibin beta A, and Cyclin D1. It also acted as an agonist of the adenosine A2A receptor and induced the phosphorylation of focal adhesion kinase, adenosine triphosphate-dependent tyrosine kinase, and mitogen-activated protein kinase. This activated signal transduction, thereby regenerating skin cells and strengthening the barrier. These results were not only observed in skin cells but also in an artificial skin model (KeraSkinTM). The use of plant-derived PDRN instead of animal-derived PDRN can promote animal welfare and environmental sustainability. Furthermore, Panax PDRN can potentially be a new plant-derived PDRN (PhytoPDRN) that may be utilized in the treatment of various skin diseases.
Homogeneous Electrochemical Aptasensor for Sensitive Detection of Zearalenone Using Nanocomposite Probe and Silica Nanochannel Film
Authors: Huang Zhongnan , Luo Xuan , Yan Fei , Zhou Bo
Published: October 2023

Developing rapid and efficient analytical methods is of great importance for food safety Herein, we present a novel homogeneous electrochemical aptasensor for ultrasensitive quantitative determination of zearalenone (ZEN) based on a nanocomposite probe and silica nanochannel film. X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and UV–Vis characterization techniques confirm that graphene oxide (GO) bears an aromatic conjugated structure, along with hydroxyl and carboxyl groups, facilitating the subsequent adsorption of cationic redox hexa-ammine-ruthenium (III) (Ru(NH3)63+) and anionic ZEN aptamer, to form a Ru(NH3)63+–ZEN aptamer–GO nanocomposite probe in a homogeneous solution. Vertically-ordered mesoporous silica films (VMSF) bearing silanol groups can be simply grown on the solid indium tin oxide (ITO) electrode surface and enable the selective preconcentration of Ru(NH3)63+, eventually leading to signal amplification. Since the detachment of Ru(NH3)63+ from the GO surface by the recognized ZEN aptamer in the presence of ZEN, more free Ru(NH3)63+ is released in solution and produces enhanced redox signals at the VMSF modified ITO electrode, allowing quantitative detection of ZEN. On the basis of the above sensing strategy, the proposed homogeneity, due to the assistance of graphene, as well as of the signal amplification and anti-fouling effects of VMSF, accurate analysis of ZEN can be realized in maize and Chinese chestnut samples.
Effects of Roasting Conditions on Antibacterial Properties of Vietnamese Turmeric (Curcuma longa) Rhizomes
Authors: Nguyen Hai Thanh , Wu Siyuan , Ootawa Tomoki , Nguyen Hieu Chi , Tran Hong Thi , Pothinuch Pitchaya , Pham Hang Thi Thu , Do Anh Thi Hong , Hoang Hao Thanh , Islam Md. Zahorul , Miyamoto Atsushi , Nguyen Ha Thi Thanh
Published: October 2023

Processing with heat treatment has been reported to alter several therapeutic effects of turmeric. In Vietnamese traditional medicine, turmeric has been long used for bacterial infections, and roasting techniques are sometimes applied with this material. However, there have been no studies investigating the effects of these thermal processes on the plant’s antibacterial properties. Our study was therefore performed to examine the changes that roasting produced on this material. Slices of dried turmeric were further subjected to light-roasting (80 °C in 20 min) or dark-roasting (160 °C in 20 min) processes. Broth dilution and agar-well diffusion methods were applied to examine and compare the effects of ethanol extracts obtained from non-roasted, light-roasted and dark-roasted samples, on a set of 6 gram-positive and gram-negative bacteria. In both investigations, dark-roasted turmeric was significantly less antibacterial than non-roasted and light-roasted materials, as evident by the higher values of minimum inhibitory concentrations and the smaller diameters of induced inhibitory zones. In addition, dark-roasting was also found to clearly reduce curcumin contents, total polyphenol values and antioxidant activities of the extracts. These results suggest that non-roasting or light-roasting might be more suitable for the processing of turmeric materials that are aimed to be applied for bacterial infections.
Novel MAGL Inhibitors Alleviate LPS-Induced Acute Kidney Injury by Inhibiting NLRP3 Inflammatory Vesicles, Modulating Intestinal Flora, Repairing the Intestinal Barrier, and Interfering with Serum Metabolism
Authors: Xiang Haixin , Wang Yangui , Yang Lan , Liu Mingfei , Sun Chenghong , Gu Yuchao , Yao Jingchun
Published: October 2023

Acute kidney injury (AKI) is a complication of a wide range of serious illnesses for which there is still no better therapeutic agent. We demonstrated that M-18C has a favorable inhibitory effect on monoacylglycerol lipase (MAGL), and several studies have demonstrated that nerve inflammation could be effectively alleviated by inhibiting MAGL, suggesting that M-18C has good anti-inflammatory activity. In this study, we investigated the effect of M-18C on LPS-induced acute kidney injury (AKI), both in vivo and in vitro, by using liquid chromatography-mass spectrometry (LC-MS), 16S rRNA gene sequencing, Western blot, and immunohistochemistry. The results showed that both in vivo and in vitro M-18C reduced the release of TNF-α and IL-1β by inhibiting the expression of NOD-like receptor thermal protein domain-associated protein 3 (NLRP3) and apoptosis-associated speck-like protein containing a CARD (ASC) protein; in addition, M-18C was able to intervene in LPS-induced AKI by ameliorating renal pathological injury, repairing the intestinal barrier, and regulating gut bacterial flora and serum metabolism. In conclusion, this study suggests that M-18C has the potential to be a new drug for the treatment of AKI.
Metabolomics Analysis Reveals the Accumulation Patterns of Flavonoids and Volatile Compounds in Camellia oleifera Petals with Different Color
Authors: Zeng Haitao , Chen Mengjiao , Zheng Tao , Tang Qi , Xu Hao
Published: October 2023

To systematically and comprehensively investigate the metabolic characteristics of coloring substances and floral aroma substances in Camellia oleifera petals with different colors, ultrahigh-performance liquid chromatography–mass spectrometry (UPLC–MS/MS) and headspace solid phase microextraction and gas chromatography–mass spectrometry (HS–SPME–GC–MS) metabolomics methods were applied to determine the metabolic profiles of white, candy-pink and dark-red petals. The results revealed that 270 volatile organic compounds were detected, mainly terpenoids, heterocyclic, esters, hydrocarbons, aldehydes, and alcohols, in which phenylethyl alcohol, lilac alcohol, and butanoic acid, 1-methylhexyl ester, hotrienol, alpha-terpineol and 7-Octen-4-ol, 2-methyl-6-methylene-, (S)-, butanoic acid, 2-methyl-, 2-methylbutyl ester, 2,4-Octadienal, (E,E)- could act as the floral scent compounds. A total of 372 flavonoid compounds were identified, and luteolin, kaempferol, cyanidin and peonidin derivatives were considered as the main coloring substances for candy-pink and dark-red petal coloration. In conclusion, this study intuitively and quantitatively exhibited the variations in flower color and floral scent of C. oleifera petal with different colors caused by changes in variations of flavonoids and volatile organic compound composition, and provided useful data for improving the sensory quality and breeding of C. oleifera petals.
Correlation between Serum 25-Hydroxyvitamin D Concentration, Monocyte-to-HDL Ratio and Acute Coronary Syndrome in Men with Chronic Coronary Syndrome—An Observational Study
Authors: Dziedzic Ewelina A. , Gąsior Jakub S. , Tuzimek Agnieszka , Dąbrowski Marek , Kochman Wacław
Published: October 2023

Cardiovascular disease (CVD) continues to be the leading cause of death in European men. Atherosclerosis and its clinical consequence, chronic coronary syndrome (CCS), comprise two main elements: dysfunction of lipoprotein metabolism and an important inflammatory component that contributes to the development of complications, including acute coronary syndrome (ACS). Measures of both components are combined in a composite marker called monocyte-to-HDL ratio (MHR). Vitamin D was previously described to influence inflammation processes, and its deficiency influences CVD risk factors. This research describes the differences in MHR and total serum 25-hydroxyvitamin D (25(OH)D) concentration between male patients with different diagnoses of CCS and the correlation between 25(OH)D and MHR in this group. Significant differences were observed between ACS and CCS patients in 25(OH)D and MHR—the highest HDL and serum 25(OH)D concentrations were observed in patients with CCS, whereas the highest value of MHR was observed in patients with STEMI. A significant correlation was observed between 25(OH)D, HDL, and MHR. Due to the significant but small nominal difference in MHR values between groups of patients diagnosed with ACS and CCS, and the possible influence of age and hyperlipidemia status on the differences in vitamin D levels in these groups, this subject requires further well-designed research. The suggested bidirectional relationship between MHR and 25(OH)D and the role of MHR as a predictor of vitamin D status in the body also needs to be verified.
Efficacy of Postprandial Exercise in Mitigating Glycemic Responses in Overweight Individuals and Individuals with Obesity and Type 2 Diabetes—A Systematic Review and Meta-Analysis
Authors: Kang Jie , Fardman Brian M. , Ratamess Nicholas A. , Faigenbaum Avery D. , Bush Jill A.
Published: October 2023

Studies investigating the acute effect of postprandial exercise (PPE) on glucose responses exhibit significant heterogeneity in terms of participant demographic, exercise protocol, and exercise timing post-meal. As such, this study aimed to further analyze the existing literature on the impact of PPE on glycemic control in overweight individuals and individuals with obesity and type 2 diabetes (T2DM). A literature search was conducted through databases such as PubMed, CINAHL, and Google Scholar. Thirty-one original research studies that met the inclusion criteria were selected. A random-effect meta-analysis was performed to compare postprandial glucose area under the curve (AUC) and 24 h mean glucose levels between PPE and the time-matched no-exercise control (CON). Subgroup analyses were conducted to explore whether the glucose-lowering effect of PPE could be influenced by exercise duration, exercise timing post-meal, and the disease status of participants. This study revealed a significantly reduced glucose AUC (Hedges’ g = −0.317; SE = 0.057; p < 0.05) and 24 h mean glucose levels (Hedges’ g = −0.328; SE = 0.062; p < 0.05) following PPE compared to CON. The reduction in glucose AUC was greater (p < 0.05) following PPE lasting >30 min compared to ≤30 min. The reduction in 24 h mean glucose levels was also greater (p < 0.05) following PPE for ≥60 min compared to <60 min post-meal and in those with T2DM compared to those without T2DM. PPE offers a viable approach for glucose management and can be performed in various forms so long as exercise duration is sufficient. The glucose-lowering effect of PPE may be further enhanced by initiating it after the first hour post-meal. PPE is a promising strategy, particularly for patients with T2DM. This manuscript is registered with Research Registry (UIN: reviewregistry1693).
Intermittent Fasting on Human Health and Disease
Authors: Margină Denisa Marilena , Drăgoi Cristina Manuela
Published: October 2023

Chronic non-communicable diseases (NCDs) are the leading cause of morbidity and mortality worldwide, but most of all in industrialized countries, and are fundamentally correlated to improper nutrition and impaired lifestyle behaviours [...]
Nutrition Care after Hospital Discharge in Singapore: Evidence-Based Best-Practice Recommendations
Authors: Ng Doris Hui Lan , Koh Frederick Hong Xiang , Yeong Hazel Ee Ling , Huey Terence Cheong Wei , Chue Koy Min , Foo Fung Joon , Chew Samuel Teong Huang
Published: October 2023

The nutritional status of hospitalised patients is often at risk or compromised and predisposed to further deterioration after discharge, leading to poor clinical outcomes, high healthcare costs, and poor quality of life. This paper aims to provide evidence-based best-practice recommendations to address this, supported by a national survey of healthcare professionals in Singapore and reviewed by a multidisciplinary expert panel under the Sarcopenia Interest Group of Society of Parenteral and Enteral Nutrition Singapore (SingSPEN). We advocate screening all patients with a validated tool which includes a disease activity/burden component, an easily accessible dietitian referral pathway for patients at risk of malnutrition, and an individualised nutrition care plan formulated and delivered using a multidisciplinary team approach for patients at risk or with malnutrition. A comprehensive team would include not only dietitians but also physicians, nurses, physiotherapists, speech therapists, and medical social workers working together towards a common goal. Information on why nutrition is important for good health and how it can be achieved should also be provided to all patients and their caregivers before and after hospital discharge. With the above recommendations, we seek to improve upon the current nutrition care processes at discharge for healthcare institutions in Singapore.
Minimal Effective Dose of Beans Required to Elicit a Significantly Lower Glycemic Response Than Commonly Consumed Starchy Foods: Predictions Based on In Vitro Digestion and Carbohydrate Analysis
Authors: Ramdath D. Dan , Renwick Simone , Hawke Aileen , Ramdath Davin G. , Wolever Thomas M. S.
Published: October 2023

Beans elicit lower glycemic responses (GRs) than other starchy foods, but the minimum effective dose (MED) to reduce GR is unknown. We sought to determine the MED of beans compared to common starchy foods. Overnight-fasted healthy volunteers consumed ¼c (phase 1, n = 24) or ½c (phase 2, n = 18) of black, cranberry, great northern, kidney, navy and pinto beans and corn, rice, pasta and potato (controls), with blood glucose measured before and for 2 h after eating. GRs (incremental areas under the curves, iAUCs) after beans were consumed were compared to those of controls by ANOVA followed by Dunnett’s test. To qualify for MED, beans had to elicit an effective reduction in GR, defined as a statistically significant reduction in iAUC of ≥20% (i.e., a relative glycemic response, RGR, ≤80). Outcomes from in vitro digestion were compared with in vivo RGR. Both doses of all six beans effectively reduced GR versus all four starchy controls, except for ¼c and ½c cranberry and pinto vs. corn, ¼c great northern and navy vs. corn and ¼c navy and pinto vs. potato. MED criteria were met for 18 comparisons of the ¼c servings, with four of the remaining six met by the ½c servings. The overall mean ± SEM RGR vs. controls was similar for the ¼c and ½c servings: 53 ± 4% and 56 ± 3%, respectively. By multiple regression analysis, RGR = 23.3 × RDS + 8.3 × SDS − 20.1 × RS + 39.5 × AS − 108.2 (rapidly digested starch, p < 0.001; slowly digested starch, p = 0.054; resistant starch, p = 0.18; available sugars, p = 0.005; model r = 0.98, p = 0.001). RGR correlated with in vitro glucose release (r = 0.92, p < 0.001). The MED of beans is ¼ cup. For n = 30 comparisons (n = 24 beans vs. controls, n = 6 controls vs. each other), an effective reduction in GR was predicted from in vitro carbohydrate analysis with 86% sensitivity and 100% specificity.