bluuebunny/crossref_metadata_2025 huggingface dataset:
Anxious Solitude, Reciprocated Friendships with Peers, and Maternal Overcontrol from Third through Seventh Grade: A Transactional Model
Authors: Gazelle Heidi , Cui Ming
Published: May 2021

Guided by a Transactional Model of anxious solitude development, we tested friend and maternal influences on continuity and change in youth anxious solitude from 3rd through 7th grade, as well as the influence of youth anxious solitude on decreased friendship participation and increased maternal overcontrol over time. Participants were 230 American youth (57% girls) selected for longitudinal study from a public-school screening sample (n = 688). Peers reported on anxious solitude, both peers and youth reported on reciprocated friendship, and youth reported on their mother’s overcontrol annually. Stability and incremental change in youth, friend, and maternal factors were tested in an autoregressive cross-lagged panel analytic model. Having few mutual friendships predicted incremental increase in youth anxious solitude in mid-elementary school, then youth anxious solitude predicted the loss of friendships after the middle school transition. Additionally, youth anxious solitude in third grade evoked increased maternal overcontrol in fourth grade, but the reverse direction of effect was not supported. Youth’s participation in few friendships also evoked mothers’ overcontrol, which exacerbated their child’s loss of friendships in elementary school. Taken together, having few mutual friends contributed to youth anxious solitude and maternal overcontrol, and subsequently these factors further exacerbated youth’s loss of friendships.
Landscape Conservation Forecasting for Data-Poor at-Risk Species on Western Public Lands, United States
Authors: Provencher Louis , Badik Kevin , Anderson Tanya , Tuhy Joel , Fletcher Dan , York Elaine , Byer Sarah
Published: May 2021

Managing vast federal public lands governed by multiple land use policies creates challenges when demographic data on at-risk species are lacking. The U.S. Bureau of Land Management Cedar City Field Office used this project in the Black Mountains (Utah) to inform vegetation management supporting at-risk greater sage-grouse and Utah prairie dog planning. Ecological systems were mapped from satellite remote sensing imagery and used to model species habitat suitability under two levels of management activity (custodial, preferred) and climate scenarios for historic and two global circulation models. Spatial state-and-transition models of ecological systems were simulated for all six scenarios up to 60 years while coupled with expert-developed habitat suitability indices. All ecological systems are at least moderately departed from reference conditions in 2012, whereas habitat suitability was 50.5% and 48.4% for sage-grouse and prairie dog, respectively. Management actions replaced non-native annual grasslands with perennial grasses, removed conifers, and controlled exotic forbs. The drier climate most affected ecological departure and prairie dog habitat suitability at 30 years only. Different climates influenced spatial patterns of sage-grouse habitat suitability, but nonspatial values were unchanged. Climate impacts on fire, vegetation succession, and restoration explain many results. Front-loading restoration is predicted to benefit under future drier climate.
Does Hyperoxia Restrict Pyrenean Rock Lizards Iberolacerta bonnali to High Elevations?
Authors: Gangloff Eric J. , Spears Sierra , Kouyoumdjian Laura , Pettit Ciara , Aubret Fabien
Published: May 2021

Ectothermic animals living at high elevation often face interacting challenges, including temperature extremes, intense radiation, and hypoxia. While high-elevation specialists have developed strategies to withstand these constraints, the factors preventing downslope migration are not always well understood. As mean temperatures continue to rise and climate patterns become more extreme, such translocation may be a viable conservation strategy for some populations or species, yet the effects of novel conditions, such as relative hyperoxia, have not been well characterised. Our study examines the effect of downslope translocation on ectothermic thermal physiology and performance in Pyrenean rock lizards (Iberolacerta bonnali) from high elevation (2254 m above sea level). Specifically, we tested whether models of organismal performance developed from low-elevation species facing oxygen restriction (e.g., hierarchical mechanisms of thermal limitation hypothesis) can be applied to the opposite scenario, when high-elevation organisms face hyperoxia. Lizards were split into two treatment groups: one group was maintained at a high elevation (2877 m ASL) and the other group was transplanted to low elevation (432 m ASL). In support of hyperoxia representing a constraint, we found that lizards transplanted to the novel oxygen environment of low elevation exhibited decreased thermal preferences and that the thermal performance curve for sprint speed shifted, resulting in lower performance at high body temperatures. While the effects of hypoxia on thermal physiology are well-explored, few studies have examined the effects of hyperoxia in an ecological context. Our study suggests that high-elevation specialists may be hindered in such novel oxygen environments and thus constrained in their capacity for downslope migration.
Sleep Disordered Breathing Measures in Early Pregnancy Are Associated with Depressive Symptoms in Late Pregnancy
Authors: Bublitz Margaret H. , Sharp Meghan , Freeburg Taylor , Sanapo Laura , Nugent Nicole R. , Sharkey Katherine , Bourjeily Ghada
Published: May 2021

Sleep disordered breathing (SDB) and depression are both common complications of pregnancy and increase risk for adverse maternal and neonatal outcomes. SDB precedes onset of depression in non-pregnant adults; however, the longitudinal relationship has not been studied in pregnancy. The present research examined temporal associations between SDB and depressive symptoms in 175 pregnant women at risk for SDB (based on frequent snoring and obesity), but without an apnea hypopnea index of ≥5 events per hour at enrollment. Women completed a self-report assessments of depressive symptoms using PHQ-9 and in-home level III sleep apnea monitoring at approximately 12- and 32-weeks’ gestation. We also assessed the risk for SDB using the Berlin Questionnaire in early pregnancy. Results revealed that measures of SDB in early pregnancy as assessed by in-home sleep study, but not by self-reported SDB, predicted elevated depressive symptoms in late pregnancy. SDB in late pregnancy was not associated with depressive symptoms. To conclude, these findings suggest that SDB may increase the risk for elevated depressive symptoms as pregnancy progresses.
Serum and Urine Biomarker Leucine-Rich Alpha-2 Glycoprotein 1 Differentiates Pediatric Acute Complicated and Uncomplicated Appendicitis
Authors: Kakar Mohit , Berezovska Marisa Maija , Broks Renars , Asare Lasma , Delorme Mathilde , Crouzen Emile , Zviedre Astra , Reinis Aigars , Engelis Arnis , Kroica Juta , Saxena Amulya , Petersons Aigars
Published: May 2021

Purpose: This prospective, single-center cohort study analyzes the potential of inflammatory protein mediator leucine-rich alpha-2 glycoprotein 1 (LRG1) for the early and accurate diagnosis of acute appendicitis (AA), and differentiation of acute complicated (AcA) from uncomplicated appendicitis (AuA). Methods: Participants were divided into the AcA, AuA, and control groups, and their serum (s-LRG1) and urine LRG1 (u-LRG1) levels were assayed preoperatively on the second and fifth postoperative days. Results: 153 patients participated, 97 had AA. Preoperative u-LRG1 with a cut-off value of 0.18 μg/mL generated an area under the receiver operated characteristic (AUC) curve of 0.70 (95% CI 0.62–0.79) for AA versus control (p < 0.001), while the results for AcA versus AuA were not significant (AUC 0.60, 95% CI 0.49–0.71, p = 0.089). The s-LRG1 levels of AA versus the control with a cut-off value of 51.69 μg/mL generated an AUC of 0.94 (95% CI 0.91–0.99, p < 0.001). The cut-off value of s-LRG1 was 84.06 μg/mL for diagnosis of AcA from AuA, and therefore, significant (AUC 0.69, 95% CI 0.59–0.80, p = 0.001). Conclusions: LRG1 exhibited excellent diagnostic performance as an inexpensive, non-invasive, rapid, and accurate biomarker able to reflect the pathogenesis of AA. LRG1 has the potential to replace advanced imaging to diagnose clinically ambiguous AA cases.
Isolation of Cell-Free miRNA from Biological Fluids: Influencing Factors and Methods
Authors: Bryzgunova Olga , Konoshenko Maria , Zaporozhchenko Ivan , Yakovlev Alexey , Laktionov Pavel
Published: May 2021

A vast wealth of recent research has seen attempts of using microRNA (miRNA) found in biological fluids in clinical research and medicine. One of the reasons behind this trend is the apparent their high stability of cell-free miRNA conferred by small size and packaging in supramolecular complexes. However, researchers in both basic and clinical settings often face the problem of selecting adequate methods to extract appropriate quality miRNA preparations for use in specific downstream analysis pipelines. This review outlines the variety of different methods of miRNA isolation from biofluids and examines the key determinants of their efficiency, including, but not limited to, the structural properties of miRNA and factors defining their stability in the extracellular environment.
Multiple Observations for Secret-Key Binding with SRAM PUFs
Authors: Kusters Lieneke , Willems Frans M. J.
Published: May 2021

We present a new Multiple-Observations (MO) helper data scheme for secret-key binding to an SRAM-PUF. This MO scheme binds a single key to multiple enrollment observations of the SRAM-PUF. Performance is improved in comparison to classic schemes which generate helper data based on a single enrollment observation. The performance increase can be explained by the fact that the reliabilities of the different SRAM cells are modeled (implicitly) in the helper data. We prove that the scheme achieves secret-key capacity for any number of enrollment observations, and, therefore, it is optimal. We evaluate performance of the scheme using Monte Carlo simulations, where an off-the-shelf LDPC code is used to implement the linear error-correcting code. Another scheme that models the reliabilities of the SRAM cells is the so-called Soft-Decision (SD) helper data scheme. The SD scheme considers the one-probabilities of the SRAM cells as an input, which in practice are not observable. We present a new strategy for the SD scheme that considers the binary SRAM-PUF observations as an input instead and show that the new strategy is optimal and achieves the same reconstruction performance as the MO scheme. Finally, we present a variation on the MO helper data scheme that updates the helper data sequentially after each successful reconstruction of the key. As a result, the error-correcting performance of the scheme is improved over time.
Spectral and Informational Analysis of Temperature and Chemical Composition of Solfatara Fumaroles (Campi Flegrei, Italy)
Authors: Tripaldi Simona , Telesca Luciano , Lovallo Michele
Published: May 2021

Temperature and composition at fumaroles are controlled by several volcanic and exogenous processes that operate on various time-space scales. Here, we analyze fluctuations of temperature and chemical composition recorded at fumarolic vents in Solfatara (Campi Flegrei caldera, Italy) from December 1997 to December 2015, in order to better understand source(s) and driving processes. Applying the singular spectral analysis, we found that the trends explain the great part of the variance of the geochemical series but not of the temperature series. On the other hand, a common source, also shared by other geo-indicators (ground deformation, seismicity, hydrogeological and meteorological data), seems to be linked with the oscillatory structure of the investigated signals. The informational characteristics of temperature and geochemical compositions, analyzed by using the Fisher–Shannon method, appear to be a sort of fingerprint of the different periodic structure. In fact, the oscillatory components were characterized by a wide range of significant periodicities nearly equally powerful that show a higher degree of entropy, indicating that changes are influenced by overlapped processes occurring at different scales with a rather similar intensity. The present study represents an advancement in the understanding of the dominant driving mechanisms of volcanic signals at fumaroles that might be also valid for other volcanic areas.
Secondary Mathematics Teachers’ Perception of Their Readiness for Emergency Remote Teaching during the COVID-19 Pandemic: A Case Study
Authors: Rodríguez-Muñiz Luis J. , Burón Diego , Aguilar-González Álvaro , Muñiz-Rodríguez Laura
Published: May 2021

The coronavirus disease 2019 (COVID-19) pandemic caused a worldwide unexpected interruption of face-to-face teaching and a sudden conversion to emergency remote teaching (ERT). In this exploratory study, a sample of 244 secondary mathematics teachers was considered to analyze their perception of their readiness to ERT during the COVID-19 pandemic based on their technological pedagogical content knowledge (TPCK), their previous training in digital teaching tools, their level of digital competence for teaching mathematics, and their adaptation to ERT. An online questionnaire was applied, and the answers were quantitatively analyzed. Given the use of a large number of digital resources and the high percentage of self-developed materials using educational software, secondary mathematics teachers reflected adequate digital competence and TPCK for teaching mathematics. The sudden transition to ERT forced teachers to slow down the pace of teaching and to reduce the content taught. Significant differences were observed based on gender and age with respect to teachers’ perception of their adaptation to ERT. Despite the positive influence of previous training on their perception of readiness for ERT, in general, teachers recognized that they need more training. The demand for preparation for video editing and online quiz composition can be considered for the design of future training programs.
Linear Active Disturbance Rejection Control Strategy with Known Disturbance Compensation for Voltage-Controlled Inverter
Authors: Li Yang , Qi Rong , Dai Mingguang , Zhang Xi , Zhao Yiyun
Published: May 2021

When the linear active disturbance rejection control (LADRC) is applied for the voltage-controlled inverter, the discrete period and the measurement noise limits the observer bandwidth, which affects the anti-disturbance performance of the system. This results in a poor ability to deal with the output voltage fluctuation under the load switch. In this paper, a novel LADRC strategy based on the known disturbance compensation is proposed for the voltage-controlled inverters. Firstly, the original LADRC scheme is designed. The dynamic performance and robustness of the system are analyzed by a root locus diagram, and the anti-disturbance ability is studied through amplitude-frequency characteristics. Then the partial model information and the load current are treated as the known disturbance and introduced to the linear extended state observer (LESO) to improve observation accuracy. The difference in anti-disturbance performance with the original scheme is compared and the stability of the LESO and LADRC is analyzed. Finally, the effectiveness of the proposed scheme is verified by the simulation and experimental results.
Intelligent Buildings in Smart Grids: A Survey on Security and Privacy Issues Related to Energy Management
Authors: Llaria Alvaro , Dos Santos Jessye , Terrasson Guillaume , Boussaada Zina , Merlo Christophe , Curea Octavian
Published: May 2021

During the last decade, the smart grid (SG) concept has started to become a reality, mainly thanks to the technical progress achieved in telecommunications, informatics and power electronics, among other domains, leading to an evolution of the traditional electrical grid into an intelligent one. Nowadays, the SG can be seen as a system of smart systems that include cyber and physical parts from different technologies that interact with each other. In this context, intelligent buildings (IBs) constitute a paradigm in which such smart systems are able to guarantee the comfort of residents while ensuring an appropriate tradeoff of energy production and consumption by means of an energy management system (EMS). These interconnected EMSs remain the objective of potential cyber-attacks, which is a major concern. Therefore, this paper conducts a survey, from a multidisciplinary point of view, of some of the main security and privacy issues related to IBs as part of the SG, including an overview of EMS, smart meters, and the main communication networks employed to connect IBs to the overall SG. Future research directions towards a security enhancement from both technical and human perspectives are also provided.
BIBC Matrix Modification for Network Topology Changes: Reconfiguration Problem Implementation
Authors: Şeker Ayşe Aybike , Gözel Tuba , Hocaoğlu Mehmet Hakan
Published: May 2021

The topology of a distribution network can be represented by a bus injection to branch current (BIBC) matrix. It has been introduced and used for load flow analysis of distribution networks. In this paper, a method for BIBC matrix modification is developed to use in applications which require a topology change representation. Proposed method that reflects the changes in configuration in the system BIBC matrix is implemented in distribution network reconfiguration problem. With providing potential solutions for network operational and planning requirements such as necessitate changes in configurations to transfer the loads to a different substation, ease the loading of equipment, conduct planned maintenance and reduce network losses during the normal operation with renewables, storage and other uprising technologies, reconfiguration may also be useful for emergencies, accidents, attacks and weather-related disasters. The BIBC modification process provides the knowledge of possible switches to open and the direction of power flow without any need to further radiality or continuity check. The proposed method needs only initial network topology information that makes it suitable to apply on any distribution network and to use with any search method or heuristic/meta-heuristic optimization algorithm. Efficiency of the method is investigated on systems with voltage dependent and time varying loads.
Green Extraction Techniques for Obtaining Bioactive Compounds from Mandarin Peel (Citrus unshiu var. Kuno): Phytochemical Analysis and Process Optimization
Authors: Šafranko Silvija , Ćorković Ina , Jerković Igor , Jakovljević Martina , Aladić Krunoslav , Šubarić Drago , Jokić Stela
Published: May 2021

In this study, an efficient utilization and valorization of mandarin peel (Citrus unshiu Marc. var. Kuno) was investigated using innovative and green extraction techniques. The first step of this study included the extraction and analysis of the volatile compounds by performing a supercritical CO2 (SC-CO2) extraction under different operating pressure conditions (100 and 300 bar). The analysis of volatile compounds of the obtained extracts was conducted by gas chromatography-mass spectrometry (GC-MS), and limonene was found to be the dominant volatile component (13.16% at 100 bar; 30.65% at 300 bar). After SC-CO2 treatment, the exhausted citrus peel waste enriched with bioactive compounds was subjected to subcritical water extraction (SWE) in a wide temperature range (130–220 °C) using different solvent-solid ratio (10–30 mL/g) in time periods from 5 to 15 min, in order to obtain bioflavonoids. Identification and quantification of present bioflavonoids was conducted by high-performance liquid chromatography with a with a diode array detector (HPLC), and hesperidin (0.16–15.07 mg/g) was determined as the most abundant flavanon in mandarin peel with other polyphenolic compounds that were possible by-products of thermal degradation. At higher temperatures, the presence of 5-hydroxymethylfurfural (5-HMF) and chlorogenic acid were detected. Antiradical activity and total phenolic content in the extracts were determined using spectrophotometric methods, while the process optimization was performed by response surface methodology (RSM).
Food Bioactives: Impact on Brain and Cardiometabolic Health—Findings from In Vitro to Human Studies
Authors: Naumovski Nenad , Sergi Domenico
Published: May 2021

Modern society is currently (and probably more than ever) immersed in the changing concept of food, seeking the beneficial functions of foods rather than only as a mean to quench hunger and support basic nutritional needs [...]
Starmerella bombicola and Saccharomyces cerevisiae in Wine Sequential Fermentation in Aeration Condition: Evaluation of Ethanol Reduction and Analytical Profile
Authors: Canonico Laura , Galli Edoardo , Agarbati Alice , Comitini Francesca , Ciani Maurizio
Published: May 2021

In the last few decades, the increase of ethanol in wine, due to global climate change and consumers’ choice is one of the main concerns in winemaking. One of the most promising approaches in reducing the ethanol content in wine is the use of non-Saccharomyces yeasts in co-fermentation or sequential fermentation with Saccharomyces cerevisiae. In this work, we evaluate the use of Starmerella bombicola and S. cerevisiae in sequential fermentation under aeration condition with the aim of reducing the ethanol content with valuable analytical profile. After a preliminary screening in synthetic grape juice, bench-top fermentation trials were conducted in natural grape juice by evaluating the aeration condition (20 mL/L/min during the first 72 h) on ethanol reduction and on the analytical profile of wines. The results showed that S. bombicola/S. cerevisiae sequential fermentation under aeration condition determined an ethanol reduction of 1.46% (v/v) compared with S. cerevisiae pure fermentation. Aeration condition did not negatively affect the analytical profile of sequential fermentation S. bombicola/S. cerevisiae particularly an overproduction of volatile acidity and ethyl acetate. On the other hand, these conditions strongly improved the production of glycerol and succinic acid that positively affect the structure and body of wine.
Black Soldier Fly (Hermetia illucens) Protein Concentrates as a Sustainable Source to Stabilize O/W Emulsions Produced by a Low-Energy High-Throughput Emulsification Technology
Authors: Wang Junjing , Jousse Morane , Jayakumar Jitesh , Fernández-Arteaga Alejandro , de Lamo-Castellví Silvia , Ferrando Montserrat , Güell Carme
Published: May 2021

There is a pressing need to extend the knowledge on the properties of insect protein fractions to boost their use in the food industry. In this study several techno-functional properties of a black soldier fly (Hermetia illucens) protein concentrate (BSFPC) obtained by solubilization and precipitation at pH 4.0–4.3 were investigated and compared with whey protein isolate (WPI), a conventional dairy protein used to stabilize food emulsions. The extraction method applied resulted in a BSFPC with a protein content of 62.44% (Kp factor 5.36) that exhibited comparable or higher values of emulsifying activity and foamability than WPI for the same concentrations, hence, showing the potential for emulsion and foam stabilization. As for the emulsifying properties, the BSFPC (1% and 2%) showed the capacity to stabilize sunflower and lemon oil-in-water emulsions (20%, 30%, and 40% oil fraction) produced by dynamic membranes of tunable pore size (DMTS). It was proved that BSFPC stabilizes sunflower oil-in-water emulsions similarly to WPI, but with a slightly wider droplet size distribution. As for time stability of the sunflower oil emulsions at 25 °C, it was seen that droplet size distribution was maintained for 1% WPI and 2% BSFPC, while for 1% BSFPC there was a slight increase. For lemon oil emulsions, BSFPC showed better emulsifying performance than WPI, which required to be prepared with a pH 7 buffer for lemon oil fractions of 40%, to balance the decrease in the pH caused by the lemon oil water soluble components. The stability of the emulsions was improved when maintained under refrigeration (4 °C) for both BSFPC and WPI. The results of this work point out the feasibility of using BSFPC to stabilize O/W emulsions using a low energy system.
Influence of Pressure Extraction Systems on the Performance, Quality and Composition of Virgin Almond Oil and Defatted Flours
Authors: Roncero José M. , Álvarez-Ortí Manuel , Pardo-Giménez Arturo , Rabadán Adrián , Pardo José E.
Published: May 2021

Almond is the most cultivated nut throughout the world. The oil content of almonds in most varieties exceeds 50%, which encourages the oil extraction to be used in gastronomy or in the cosmetic industry. The preferred system to extract almond oil is by means of pressure, which leads to obtaining a virgin oil ready for consumption. In this work, almond oil has been obtained using two pressure systems: screw press (SP) and hydraulic press (HP). The performance of both methods, as well as their influence on quality and composition characteristics of the almond oils obtained are analyzed from both a physical-chemical and sensory point of view. From an industry perspective, the highest oil yield is obtained with the SP when it operates at temperatures of 100–150 °C. Regarding the quality and chemical composition, the oils obtained by HP showed better quality indices, as they are subjected to a less aggressive treatment without influence of temperature, but lower content in total sterols. Fatty acid pattern, characterized by the predominance of unsaturated fatty acids (>90%), was not affected by the pressing system. The different operational conditions tested did not greatly affect the performance or composition of the oils obtained, but sensory tests showed two clearly differentiated products, the oil obtained by HP and that obtained by SP, according to consumer preferences. The defatted almond flours obtained as a by-product of the oil extraction process are characterized by a high content in protein and fiber, and a higher content in fat when the flour is produced from the pressing cake of HP.
Identification, Comparison and Classification of Volatile Compounds in Peels of 40 Apple Cultivars by HS–SPME with GC–MS
Authors: Yang Shunbo , Hao Nini , Meng Zhipeng , Li Yingjuan , Zhao Zhengyang
Published: May 2021

Aroma is an important quality indicator for apples and has a great influence on the overall flavour and consumer acceptance. However, the information of the aroma volatile compounds in apple peels is largely unknown. In this study, evaluation of volatile compounds in peels of 40 apple cultivars was carried out using headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). A total of 78 volatile compounds were identified, including 47 esters, 12 aldehydes, 5 alcohols, 3 ketones, 1 acid and 10 others. Eight volatile compounds were common in all apple cultivars. Cultivar Changfu No. 2 contained the highest number of volatile compounds (47), while Qinyue contained the least (20). Honey Crisps had the highest volatile content, at 27,813.56 ± 2310.07 μg/kg FW, while Huashuo had the lowest volatile content, at 2041.27 ± 120.36 μg/kg FW. Principal component analysis (PCA) clustered the 40 apple cultivars into five groups. Aroma is cultivar-specific, volatile compounds of hexyl butyrate, hexyl 2-methylbutyrate and hexyl hexanoate, together with hexanal, (E)-2-hexenal, 1-hexanol, estragole and α-farnesene could be proposed for apple cultivar classification in the future.
Pre-Crystallization of Nougat by Seeding with Cocoa Butter Crystals Enhances the Bloom Stability of Nougat Pralines
Authors: Böhme Birgit , Bickhardt Annika , Rohm Harald
Published: May 2021

Fat bloom is an outstanding quality defect especially in filled chocolate, which usually comprises oils of different origins and with different physical properties. Dark chocolate pralines filled with nougat contain a significant amount of hazelnut oil in their center and have been reported as being notably susceptible to oil migration. The current study was designed to test the assumption that a targeted crystallization of nougat with cocoa butter seed crystals is an appropriate technological tool to reduce filling oil transfer to the outside of the praline and, hence, to counteract chocolate shell weakening and the development of fat bloom. For this purpose, the hardness of nougat/chocolate layer models and the thermal properties of chocolate on top of nougat were analyzed during storage at 23 °C for up to 84 days. Pronounced differences between layer models with seeded nougat and with control nougat that was traditionally tempered were observed. The facts that chocolate hardness increased rather than decreased during storage, that the cocoa butter melting peak was shifted towards a lower temperature, and that the hazelnut oil content in the chocolate was reduced can be taken as explicit indicators for the contribution of seeded nougat to the fat bloom stability of filled chocolate.
Expanding the Phenotypic and Genotypic Spectrum of Bietti Crystalline Dystrophy
Authors: da Palma Mariana Matioli , Motta Fabiana Louise , Salles Mariana Vallim , Texeira Caio Henrique Marques , Gomes André V. , Casaroli-Marano Ricardo , Sallum Juliana Maria Ferraz
Published: May 2021

The rare form of retinal dystrophy, Bietti crystalline dystrophy, is associated with variations in CYP4V2, a member of the cytochrome P450 family. This study reports patients affected by typical and atypical Bietti crystalline dystrophy, expanding the spectrum of this disease. This is an observational case series of patients with a clinical and molecular diagnosis of Bietti crystalline dystrophy that underwent multimodal imaging. Four unrelated patients are described with two known variants, c.802-8_810del17insGC and c.518T > G (p.Leu173Trp), and one novel missense variant, c.1169G > T (p.Arg390Leu). The patient with the novel homozygous variant had the most severe phenotype resulting in macular hole formation and retinal detachment in both eyes. To the best of our knowledge, there is no association of these features with Bietti crystalline dystrophy. Patient 1 was the youngest patient and had the mildest phenotype with crystals in the retina without chorioretinal atrophy and visual complaints. Patients 2 and 3 presented with fewer crystals and chorioretinal atrophy. These three patients presented a classic phenotype. The fourth patient presented with an atypical and severe phenotype. This study reveals a new genotype and new phenotype associated with this disorder.
SLC26A2-Associated Diastrophic Dysplasia and rMED—Clinical Features in Affected Finnish Children and Review of the Literature
Authors: Härkönen Helmi , Loid Petra , Mäkitie Outi
Published: May 2021

Diastrophic dysplasia (DTD) is a rare osteochondrodysplasia characterized by short-limbed short stature and joint dysplasia. DTD is caused by mutations in SLC26A2 and is particularly common in the Finnish population. However, the disease incidence in Finland and clinical features in affected individuals have not been recently explored. This registry-based study aimed to investigate the current incidence of DTD in Finland, characterize the national cohort of pediatric subjects with DTD and review the disease-related literature. Subjects with SLC26A2-related skeletal dysplasia, born between 2000 and 2020, were identified from the Skeletal dysplasia registry and from hospital patient registry and their clinical and molecular data were reviewed. Fourteen subjects were identified. Twelve of them were phenotypically classified as DTD and two, as recessive multiple epiphyseal dysplasia (rMED). From the subjects with available genetic data, 75% (9/12) were homozygous for the Finnish founder mutation c.-26+2T>C. Two subjects with rMED phenotype were compound heterozygous for p.Arg279Trp and p.Thr512Lys variants. The variable phenotypes in our cohort highlight the wide spectrum of clinical features, ranging from a very severe form of DTD to milder forms of DTD and rMED. The incidence of DTD in Finland has significantly decreased over the past decades, most likely due to increased prenatal diagnostics.
Genome-Wide Association Study Identified Novel Candidate Loci/Genes Affecting Lodging Resistance in Rice
Authors: Meng Bingxin , Wang Tao , Luo Yi , Xu Deze , Li Lanzhi , Diao Ying , Gao Zhiyong , Hu Zhongli , Zheng Xingfei
Published: May 2021

Lodging reduces rice yield, but increasing lodging resistance (LR) usually limits yield potential. Stem strength and leaf type are major traits related to LR and yield, respectively. Hence, understanding the genetic basis of stem strength and leaf type is of help to reduce lodging and increase yield in LR breeding. Here, we carried out an association analysis to identify quantitative trait locus (QTLs) affecting stem strength-related traits (internode length/IL, stem wall thickness/SWT, stem outer diameter/SOD, and stem inner diameter/SID) and leaf type-associated traits (Flag leaf length/FLL, Flag leaf angle/FLA, Flag leaf width/FLW, leaf-rolling/LFR and SPAD/Soil, and plant analyzer development) using a diverse panel of 550 accessions and evaluated over two years. Genome-wide association study (GWAS) using 4,076,837 high-quality single-nucleotide polymorphisms (SNPs) identified 89 QTLs for the nine traits. Next, through “gene-based association analysis, haplotype analysis, and functional annotation”, the scope was narrowed down step by step. Finally, we identified 21 candidate genes in 9 important QTLs that included four reported genes (TUT1, OsCCC1, CFL1, and ACL-D), and seventeen novel candidate genes. Introgression of alleles, which are beneficial for both stem strength and leaf type, or pyramiding stem strength alleles and leaf type alleles, can be employed for LR breeding. All in all, the experimental data and the identified candidate genes in this study provide a useful reference for the genetic improvement of rice LR.
Improved Balance and Gait Ability and Basic Activities of Daily Living after Comprehensive Geriatric Care in Frail Older Patients with Fractures
Authors: Meyer Marco , Schmetsdorf Stefanie , Stein Thomas , Niemoeller Ulrich , Arnold Andreas , Reuter Iris , Kostev Karel , Grünther Ralf-Achim , Tanislav Christian
Published: May 2021

(1) Purpose: Comprehensive geriatric care (CGC) is a multidisciplinary treatment approach for elderly patients. We aimed to investigate outcomes in fracture patients who had been treated using this approach in a large geriatric unit. (2) Methods: This observational cohort study assessed the gait function (using the Tinetti Balance and Gait Test (TBGT)) and basic activities of daily living (ADL) (using the Barthel index (BI)) before and after CGC and compared the results. Baseline data, walking ability assessments (Timed Up and Go, TUG), and cognitive status (mini mental status examination, MMSE) were also analyzed in the subgroup of patients with versus without fractures. (3) Results: Out of 1263 hospitalized patients, 1099 received CGC (median age: 83.1 years (IQR: 79.0–87.8 years); 64.1% were female). TBGT improvement was observed in 90.7% and BI increased in 82.7% of fracture patients. A TBGT improvement of >5 was noted in 47.3% and was associated with female sex, a lower BI at admission (median: 40 versus 45; p = 0.010), and poorer mobility on admission (TUG: median 5 versus 4; p = 0.001). An improvement in BI of ≥15 was observed in 63.0% of the cases, and was associated with a better cognitive status (MMSE: median 25 versus 18; p = 0.001) and inversely associated with diabetes mellitus and a previous stroke. (4) Conclusion: CGC in specialized geriatric units improves the balance and gait and the basic ADL in geriatric patients. After fracture, female patients are more likely to experience improvements in gait and balance, while patients with better cognitive condition are more likely to experience improvements in ADL.
Determinants of Satisfaction with Services, and Trust in the Information Received in Community Pharmacies: A Comparative Analysis to Foster Pharmaceutical Care Adoption
Authors: Druică Elena , Ianole-Călin Rodica , Băicuș Cristian , Dinescu Raluca
Published: May 2021

Patient’s satisfaction with community pharmacy services, and patients’ trust in the information received in community pharmacies are important drivers of pharmaceutical care adoption. An online questionnaire assessing patient satisfaction with the services received in pharmacies and trust in the pharmacist’s advice, along with their determinants, was administered to 343 Romanian chronic and non-chronic patients. Using various statistical tests, exploratory factor analysis, and robust regression we explored determinants of satisfaction and trust. We found that satisfaction with services is predicted by pharmacists’ attitude (β = 631, p < 0.001), low waiting time (β = 0.180, p < 0.001), affordable cost of the drugs (β = 0.09, p = 0.009), and drug availability (β = 0.157, p < 0.001). At the same time, trust in the information received is driven by pharmacists’ attention (β = 0.610, p < 0.001), whether the patient received precautionary information (β = 0.425, p < 0.001), low waiting time (β = 0.287, p < 0.001), and whether the respondent is a chronic patient or not (non-chronic patients express more trust, β = 0.328, p = 0.04). Our study expands the existing paradigm that sees trust as a simple predictor of satisfaction by showing that trust and satisfaction are predicted by different variables, and thus they should be addressed using different strategies. In fact, we found that they share only one predictor—waiting time, highly significant in both cases. Our findings show that, without prioritizing trust in the information received in community pharmacies to reduce information asymmetry between patient and pharmacist, the focus only on patient satisfaction may lead to a scenario in which community pharmacies will end up to be better integrated in the business sector and not in the public health system.
Experience of Intimate Partner Violence among Women in Sexual Unions: Is Supportive Attitude of Women towards Intimate Partner Violence a Correlate?
Authors: Aboagye Richard Gyan , Okyere Joshua , Seidu Abdul-Aziz , Hagan John Elvis , Ahinkorah Bright Opoku
Published: May 2021

Intimate partner violence (IPV) is predominant in sub-Saharan Africa (SSA), with nearly 40 percent of women reporting IPV at some point in time. In this study, we investigated whether a supportive attitude towards IPV is associated with past-year experience of IPV among women in sexual unions in SSA. This study involved a cross-sectional analysis of data from the Demographic and Health Survey (DHS) of 23 countries in SSA. Bivariate and multivariable binary logistic regression analyses were performed to determine the association between attitude towards IPV and past-year experience of IPV. The regression results were presented in a tabular form using crude odds ratio (cOR) and adjusted odds ratio (aOR) at 95% confidence intervals (CIs). In the pooled countries, we found that women who had supportive attitude towards IPV were more likely to experience IPV compared to those who rejected IPV (cOR = 1.72, 95% CI = 1.64, 1.79), and this persisted after controlling for maternal age, marital status, wealth, maternal education level, place of residence, and mass-media exposure (aOR = 1.72, 95% CI = 1.64, 1.79). The same trend and direction of association between attitude towards IPV and experience of IPV was also found in all the 23 studied countries. This study has demonstrated that women who accept IPV are more likely to experience IPV. Hence, we recommend that efforts to end IPV must focus primarily on changing the attitudes of women. This goal can be achieved by augmenting women’s empowerment, education, and employment interventions, as well as sensitizing women in relation to the deleterious ramifications of accepting IPV. Furthermore, reducing IPV is critical towards the achievement of Sustainable Development Goal 3.
Being Both a Parent and a Healthcare Worker in the Pandemic: Who Could Be Exhausted More?
Authors: Çakmak Güzin , Öztürk Zeynel Abidin
Published: May 2021

(1) Background: The COVID-19 pandemic has changed the living conditions of many people. Many people felt significantly constrained. However, for individuals who are both parents and healthcare professionals, the situation seems more troubling in other ways. (2) Objectives: Based on this, we planned a study evaluating demographic characteristics, COVID-19-related anxiety levels, and parenting-related stress levels of the health care professionals who were working in the University Hospital. We also evaluated the parameters that affect COVID-19-related anxiety and parenting stress. (3) Methods: The level of COVID-19-related anxiety is assessed by the coronavirus anxiety scale. The parenting stress index-short form is used for evaluation of parenting stress. Statistical analysis was done by SPSS version 22. (4) Results: Female gender, working as a nurse, a history of COVID-19, and having a child attending daycare were parameters that increased the level of COVID-19-related anxiety. Occupation, being a parent of a schoolchild and/or primary school child, being a parent of a child receiving face-to-face education, and having more than two children were found to be risk factors for parenting stress. Clinically significant parenting stress was found to increase threefold in healthcare workers with more than two children (R2 = 0.101, p = 0.039). (5) Conclusions: Healthcare professionals, who are also parents, play a huge role both at home and in the hospital in the pandemic. Therefore, it is inevitable that their stress and anxiety levels increase. It is important to determine the factors that cause stress and anxiety and to take measures in this direction to get through this process well.
Detecting Maturity in Fresh Lycium barbarum L. Fruit Using Color Information
Authors: Zhao Jian , Chen Jun
Published: May 2021

The accurate quantitative maturity detection of fresh Lycium barbarum L. (L. barbarum) fruit is the key to determine whether fruit are suitable for harvesting or not and can also be helpful to improve the quality of post-harvest processing. To achieve this goal, abnormal samples were eliminated by the Mahalanobis Distance (MD), and nine components (i.e., R, G, B, H, S, V, L, a, and b) of the ripe fruit, half-ripe fruit, and unripe fruit were extracted, firstly. Then, significant component combinations of the three fruits beneficial to the extraction of their areas were determined. Through binary processing, morphology processing, and other image processing methods, a quantitative maturity detection model of fruit was established based on the support vector machine (SVM) model. On this basis, field experiments were conducted to verify and compare the relationship between the prediction results of the model and the picking forces of fruit. Field experiments showed that the accuracies of both the training set and prediction set were 100% and the prediction results of the model were consistent with the picking forces of fruit. Findings provided a theoretical basis for the accurate quantitative maturity detection of fresh L. barbarum fruit.
Influence of Personal Protective Equipment on Wildland Firefighters’ Physiological Response and Performance during the Pack Test
Authors: Carballo-Leyenda Belén , Gutiérrez-Arroyo Jorge , García-Heras Fabio , Sánchez-Collado Pilar , Villa-Vicente José G. , Rodríguez-Marroyo Jose A.
Published: May 2021

The Pack Test (PT) is a widely used test to establish readiness for work in wildland firefighting. It is common to perform this test dressed in regular exercise clothing. However, wildland firefighters (WFF) have to wear personal protective equipment (PPE) during their deployments, which increases the physiological strain and reduces their work capacity. This study aimed to analyse the impact of full PPE on PT performance. Nine male professional WFF performed in random order a PT walking at the fastest possible self-pace wearing two different clothing configurations: (i) traditional short sports gear (SG) and (ii) the PPE currently used by Spanish WFF. Heart rate (HR), rating of perceived exertion and lap time were recorded during the PT. In addition, oxygen uptake (VO2) was estimated through the individual VO2–HR relationship previously obtained during a graded exercise test. There was a significant decrease in the PT performance (i.e., completion time) (~12%, p < 0.05) in PPE. The physiological demands with this configuration were significantly higher (~10%, p < 0.05). WFF spent ~13 min above the anaerobic threshold in PPE vs. ~4 min in SG. A multiple stepwise regression analysis revealed that PT performance variation might be explained by the maximal aerobic velocity (84.5%) in PPE and the VO2max (75.9%) in SG. In conclusion, wearing complete PPE increases WFF’s physiological strain, which translates into a significant PT performance reduction. Performing the test walking at the fastest possible self-pace wearing the PPE would better reflect the high-intensity effort periods reported in real scenarios.
The Entry Lag of Innovative Drugs in Russia, 2010–2019
Authors: Chaplenko Alexander , Gildeeva Geliya , Vlassov Vasiliy
Published: May 2021

Objective: Evaluation of the lag timelines for the launch of innovative drugs to the Russian market and pharmacoeconomic factors they can depend on. Methods: To complete the investigation, we used information about drug products, namely, dates of submission and approval, and pharmacological groups recovered from national registers and official databases. Results: Due to impacts of market factors and imperfection of the state regulation, original drugs developed abroad enter the Russian market a few years after their registration in the United States of America, the European Union, and Japan. The average time from the moment of initial approval of a drug in the aforementioned countries and jurisdictions to the moment of registration in Russia is 4 years and 8 months, with a median value of 2.5 years. It has been shown that half of this term is spent on the performance of the procedures of the expertise of the drug registration dossier in the Russian Federation. Conclusion: To attain the goal of adequate supplies to the population of the Russian Federation of the most up-to-date, high quality, safe, and efficacious medications, apart from the support of national originators of innovative drugs, we are required to upgrade the existing system of original drug registration. Improvement should be primary focused on the drugs already approved by the leading national regulatory authorities in order to ensure innovative medicine access for Russian patients.