bluuebunny/crossref_metadata_2025 huggingface dataset:
Carrots or sticks? Analyzing the application of US economic statecraft towards Latin American engagement with China
Authors: Zelicovich Julieta , Yamin Patricio
Published: April 2024

Geopolitical competition between the United States and China has led to an increased reliance on economic statecraft. In this context, understanding the conditions that trigger trade, aid, or investment weaponization becomes crucial. This article examines how the United States has employed economic statecraft in response to Latin American countries’ engagement with China. The study revisits the theoretical debate on positive and negative economic statecraft and proposes a mechanism that identifies the conditions under which “carrots” or “sticks” are more likely to be employed. We argue that the US response towards Latin American countries’ engagement with China tends to prioritize economic engagement over economic coercion, particularly when dealing with countries that are politically and economically aligned with Washington policies. To test our argument, we adopt a mixed-methods approach. First, we conduct a case study analysis on the United States-Panama relationship. Second, we perform a statistical analysis to assess the impact of economic engagement with China on the allocation of American foreign assistance in the region.
On Accountability and Hierarchy
Authors: BENDOR JONATHAN , SWISTAK PIOTR
Published: April 2024

Democracy promises accountability via elections; bureaucracy promises coordination via hierarchy. Many scholars believe these properties conflict. We prove, however, that accountability is precisely what unifies democracy and meritocratic (Weberian) bureaucracy. Central to the concept of meritocracy are performance reviews. We prove that a review system where all individuals and groups are accountable must also be democratic. Thus, meritocratic hierarchy, accountability, and democracy are intertwined. But accountability in modern political systems confronts a significant issue. Such systems include many knowledge-intensive specialties, and since specializations are limited to some but not all members of an institution, the full accountability of democracies entails review of specialists by amateurs. We prove that modern political systems necessarily exhibit this tension. It is a hallmark of modern institutions rather than a problem to be solved.
Our experience of a speech and language therapist-led swallow disorder clinic
Authors: Smith Joseph , Smith Anita , McInally Karen , Kirkland Paul
Published: April 2024

Introduction

Dysphagia can lead to morbidity including weight loss and aspiration pneumonia. Effective triage of patients and streamlining of pathways to expedite diagnosis and treatment is therefore imperative.

Objectives

The goals of this research were to measure the referral to treatment time for dysphagia patients in a newly established pathway and compare with existing UK national and local referral to treatment times, and to evaluate patient feedback.

Methods

Speech and language therapy advanced clinical practitioners were trained in nasendoscopy and assessment of swallow. Referral to treatment times were measured and patient satisfaction questionnaires completed.

Results

A decrease in triage to treatment time (from 24 to 6 weeks). Patients reported high understanding of the condition and minimal discomfort during assessments. Radiation exposure was reduced (2 per cent of patients undergoing soluble contrast swallows, previously 100 per cent).

Conclusion

The new pathway expedites treatment and achieves high patient satisfaction. It empowers speech and language therapy in efficiently managing low-complexity cases and supports multidisciplinary care for dysphagia patients.
Falsity and untruth
Authors: van Inwagen Peter
Published: April 2024

Jc Beall's Divine Contradiction is a fascinating defence of the idea that contradictions are true of the tri-personal God. This project requires a logic that avoids the consequence that every proposition follows from a contradiction. Beall presents such a logic. This ‘gap/glut’ logic is the topic of this article. A gap/glut logic presupposes that falsity is not simply the absence of truth – for a proposition that is true may also be false. This article is essentially an examination of the idea that falsity is not simply untruth. The author rejects this position but does not claim to have an argument against it. In lieu of an argument, he presents three ‘considerations’. First, it is possible to give an intuitive semantics for the language of sentential logic that yields ‘classical’ sentential logic (including ‘p, ¬ p ⊢ q’) and which makes no mention of truth-values. Second, it is possible to imagine a race who manage their affairs very well without having the concept ‘falsity’. Third, it is possible to construct a semantics that yields a logic identical with the dialetheist logic and which makes no mention of truth-values – and which, far from being plausible, seems pointless.
Unravelling the nutritional transition in Spain: From meat shortages to excess (1958–1990)
Authors: Perea Pablo Delgado
Published: April 2024

The nutritional transition, together with the demographic and epidemiological transitions, stands as one of the most crucial phenomena shaping societies in the 20th century. A prominent characteristic of the nutritional transition is the increased consumption of animal-origin protein, particularly meat. Within this context, the present article utilises Spain as a case study to provide a close examination of the nutritional transition during the latter half of the 20th century. Spain’s significance lies in its late but rapid development of this transition. In the 1960s, meat consumption was relatively low compared to other European countries; however, within just two decades, it surpassed that of many developed nations. On one hand, the article offers a detailed insight into how budget constraints were eased to foster meat consumption among various consumer groups. On the other hand, the study aims to quantify the influence of income, prices, and preferences in driving this process
A parental communication assessment initiative in the paediatric cardiovascular ICU
Authors: Hansen Katherine , Jenkins Erin , Zhu Aihua , Collins Shawna , Williams Kimberly , Garcia Ariadna , Weng Yingjie , Kaufman Beth , Sacks Loren D. , Cohen Harvey , Shin Andrew Y. , Patel Meghna D.
Published: April 2024

Objective:

Challenges to communication between families and care providers of paediatric patients in intensive care units (ICU) include variability of communication preferences, mismatched goals of care, and difficulties carrying forward family preferences from provider to provider. Our objectives were to develop and test an assessment tool that queries parents of children requiring cardiac intensive care about their communication preferences and to determine if this tool facilitates patient-centred care and improves families’ ICU experience.

Design:

In this quality improvement initiative, a novel tool was developed, the Parental Communication Assessment (PCA), which asked parents with children hospitalised in the cardiac ICU about their communication preferences. Participants were prospectively randomised to the intervention group, which received the PCA, or to standard care. All participants completed a follow-up survey evaluating satisfaction with communication.

Main Results:

One hundred thirteen participants enrolled and 56 were randomised to the intervention group. Participants who received the PCA preferred detail-oriented communication over big picture. Most parents understood the daily discussions on rounds (64%) and felt comfortable expressing concerns (68%). Eighty-six percent reported the PCA was worthwhile. Parents were generally satisfied with communication. However, an important proportion felt unprepared for difficult decisions or setbacks, inadequately included or supported in decision-making, and that they lacked control over their child’s care. There were no significant differences between the intervention and control groups in their communication satisfaction results.

Conclusions:

Parents with children hospitalised in the paediatric ICU demonstrated diverse communication preferences. Most participants felt overall satisfied with communication, but individualising communication with patients’ families according to their preferences may improve their experience.
THE HISTORY OF ECONOMICS SOCIETY AT FIFTY: WHAT KIND OF DIVERSITY?
Authors: Fontaine Philippe
Published: April 2024

My first acquaintance with the History of Economics Society (HES) occurred in the summer of 1990 when I attended its seventeenth annual meeting. Organized by the then HES president and now recently deceased Stanley “Todd” Lowry at Washington and Lee University in Lexington, Virginia, it marked the beginning of my experience with US higher education. Later that summer, I began a two-year post-doctoral fellowship at Harvard University. The economics department still hosted a history of economics seminar—the Kress seminar—though its actual connection with the seminar was tenuous.1 Following the Lexington meeting, the paper I presented was accepted for publication in volume 7 of Perspectives on the History of Economic Thought—a book series that published selected papers from the HES conference. It was my first publication in English. From then on, I kept attending the HES annual meeting regularly and with enthusiasm. I met a number of stimulating people and made some good friends there. It was always refreshing to find myself in the United States following nine months of teaching and research in France.
Introduction to the 20th Anniversary Special Issue of the Journal of Pension Economics and Finance
Authors: Rauh Joshua
Published: April 2024

The papers in this 20th Anniversary Special Issue reflect to a large extent how the fields of pension economics and pension finance have developed in the past two decades, although there remain very clear connections to the research published in the Journal's first issue. While there has been great progress in research on pensions and retirement economics over the last 20 years, there remain important outstanding questions for future study.
The British (and International?) Music Society: Australian Identity and Musical Internationalism in Interwar Sydney
Authors: KIRBY SARAH
Published: April 2024

In the aftermath of the First World War, Henri Verbrugghen founded the first Australian branch of the British Music Society. The BMS – a product of post-First World War cultural renewal – was established in London to ‘champion the cause of British composers at home and abroad’. In Sydney, these aspirations extended to promoting music from Australia, and through affiliation with the International Society for Contemporary Music, music of the rest of the world. This article explores the activities of the Sydney BMS, situating these within contemporary discourses of nationalism and internationalism in the construction of interwar Australian cultural identity. It argues that the sometimes conflicting aims of the society reflect a wider political and social ambivalence about Australia's place in the international landscape. While its primary goal was to champion local composers, this was held in uneasy balance with a desire to promote British music ‘proper’.
Supercells and Tornado‐Like Vortices in an Idealized Global Atmosphere Model
Authors: Cheng Kai‐Yuan , Lin Shian‐Jiann , Harris Lucas , Zhou Linjiong
Published: April 2024

We investigate the representation of individual supercells and intriguing tornado‐like vortices in a simplified, locally refined global atmosphere model. The model, featuring grid stretching, can locally enhance the model resolution and reach cloud‐resolving scales with modest computational resources. Given a conditionally unstable sheared environment, the model can simulate supercells realistically, with a near‐ground vortex and funnel cloud at the center of a rotating updraft reminiscent of a tornado. An analysis of the Eulerian vertical vorticity budget suggests that the updraft core of the supercell tilts horizontal vorticity into the tornado‐like vortex, which is then amplified through vertical stretching by the updraft. Results suggest that the simulated vortex is dynamically similar to observed tornadoes, as well as those simulated in modeling studies at much higher horizontal resolution. Lastly, we discuss the prospects for the study of cross‐scale interactions involving supercells.
Evaluation of Wildfire Plume Injection Heights Estimated from Operational Weather Radar Observations Using Airborne Lidar Retrievals
Authors: Krishna M. , Saide P. E. , Ye X. , Turney F. A. , Hair J. W. , Fenn M. , Shingler T.
Published: April 2024

The vertical distribution of wildfire smoke aerosols is important in determining its environmental impacts but existing observations of smoke heights generally do not possess the temporal resolution required to fully resolve the diurnal behavior of wildfire smoke injection. We use Weather Surveillance Radar‐1988 Doppler (WSR‐88D) dual polarization data to estimate injection heights of Biomass Burning Debris (BBD) generated by fires. We detect BBD as a surrogate for smoke aerosols, which are often collocated with BBD near the fire but are not within the size range detectable by these radars. Injection heights of BBD are derived for 2–10 August 2019, using WSR‐88D reflectivity (Z ≥ 10 dBZ) and dual polarization correlation coefficients (0.2 < C.C < 0.9) to study the Williams Flats fire. Results show the expected diurnal cycles with maximum injection heights present during the late afternoon period when the fire's intensity and convective mixing are maximized. WSR‐88D and airborne lidar injection height comparisons reveal that this method is sensitive to outliers and generally overpredicts maximum heights by 40%, though mean and median heights are better captured (<20% mean error). WSR‐88D heights between the 75th and 90th percentile seem to accurately represent the maximum heights, with the exception of heights estimated during the occurrence of a pyro‐cumulonimbus. Location specific mapping of WSR‐88D and lidar injection heights reveal that they diverge further away from the fire as expected due to BBD settling. Most importantly, WSR‐88D‐derived injection height estimates provide near continuous smoke height information, allowing for the study of diurnal variability of smoke injections.
An Approach for Assessing Human Respiration CO2 Emissions Using Radiocarbon Measurements and Bottom‐Up Data Sets
Authors: Wang Peng , Zhou Weijian , Niu Zhenchuan , Huo Da , Zhou Jie , Li Haogen , Cheng Peng , Wu Shugang , Xiong Xiaohu , Chen Ning
Published: April 2024

Carbon dioxide (CO2) is a major greenhouse gas in the atmosphere and has large impacts on climate change. Its fossil fuel (CO2ff) and biogenic (CO2bio) sources are well investigated, while CO2 emissions from human respiration (CO2hr), a subset of CO2bio, have received less attention. Especially as a source of carbon emissions in densely populated megacities, the role of CO2hr emissions in the carbon cycles was largely neglected. Here we fully characterize the respiratory CO2 emission rates (CERs) of Chinese people for the first time. Using the example of the megacity Beijing in China, we estimate the CO2hr emissions and present a method for quantifying its fraction in the atmospheric CO2 based on radiocarbon (14C) measurements and inventory data sets. The results show that males and females have similar age trends in CERs, but the gender difference is significant, especially between the ages of 20 and 60, the average CERs was 33% higher for males than for females (P < 0.05). The CO2hr emissions were about 22.2 ± 0.6 kt CO2 per day, which was equivalent to 7.5% of daily CO2ff emissions in winter. The proportion is likely to be twice in summer due to the seasonal fluctuations of fossil fuel emissions. More importantly, the respiratory emissions could increase atmospheric CO2 concentration by about 2 ppm, accounting for 14% ± 6% of average CO2bio concentration in winter. This study highlights the importance of human respiration in carbon emissions in megacities and has implications for a better understanding of the regional carbon budget.
Thank You to Our 2023 Peer Reviewers
Authors: Balikhin Michael , Ganjushkina Natalia , Pierrard Viviane , Song Paul , Zong Qiu‐Gang
Published: April 2024

Key Points

The editors thank the 2023 peer reviewers
Clonal hematopoiesis–derived therapy-related myeloid neoplasms after autologous hematopoietic stem cell transplant for lymphoid and non-lymphoid disorders
Authors: Awada Hussein , Gurnari Carmelo , Visconte Valeria , Durmaz Arda , Kuzmanovic Teodora , Awada Hassan , Tu Zheng Jin , Cook James R. , Bolwell Brian J. , Sobecks Ronald , Kalaycio Matt , Bosler David , Maciejewski Jaroslaw P.
Published: April 2024

Therapy-related myeloid neoplasms (tMN) are complications of cytotoxic therapies. Risk of tMN is high in recipients of autologous hematopoietic stem cell transplantation (aHSCT). Acquisition of genomic mutations represents a key pathogenic driver but the origins, timing and dynamics, particularly in the context of preexisting or emergent clonal hematopoiesis (CH), have not been sufficiently clarified. We studied a cohort of 1507 patients undergoing aHSCT and a cohort of 263 patients who developed tMN without aHSCT to determine clinico-molecular features unique to post-aHSCT tMN. We show that tMN occurs in up to 2.3% of patients at median of 2.6 years post-AHSCT. Age ≥ 60 years, male sex, radiotherapy, high treatment burden ( ≥ 3 lines of chemotherapy), and graft cellularity increased the risk of tMN. Time to evolution and overall survival were shorter in post-aHSCT tMN vs. other tMN, and the earlier group’s mutational pattern was enriched in PPM1D and TP53 lesions. Preexisting CH increased the risk of adverse outcomes including post-aHSCT tMN. Particularly, antecedent lesions affecting PPM1D and TP53 predicted tMN evolution post-transplant. Notably, CH-derived tMN had worse outcomes than non CH-derived tMN. As such, screening for CH before aHSCT may inform individual patients’ prognostic outcomes and influence their prospective treatment plans. Presented in part as an oral abstract at the 2022 American Society of Hematology Annual Meeting, New Orleans, LA, 2022.
Lithium rescues cultured rat metatarsals from dexamethasone-induced growth failure
Authors: Soucek Ondrej , Cinek Ondrej , Velentza Lilly , Semjonov Valerij , Bezdicka Martin , Zaman Farasat , Sävendahl Lars
Published: April 2024

Background

Glucocorticoids are commonly used in children with different chronic diseases. Growth failure represents a so far untreatable undesired side-effect. As lithium chloride (LiCl) is known to induce cell renewal in various tissues, we hypothesized that LiCl may prevent glucocorticoid-induced growth failure.

Methods

We monitored growth of fetal rat metatarsals cultured ex-vivo with dexamethasone and/or LiCl, while molecular mechanisms were explored through RNA sequencing by implementing the differential gene expression and gene set analysis. Quantification of β-catenin in human growth plate cartilage cultured with dexamethasone and/or LiCl was added for verification.

Results

After 14 days of culture, the length of dexamethasone-treated fetal rat metatarsals increased by 1.4 ± 0.2 mm compared to 2.4 ± 0.3 mm in control bones (p < 0.001). The combination of LiCl and dexamethasone led to bone length increase of 1.9 ± 0.3 mm (p < 0.001 vs. dexamethasone alone). By adding lithium, genes for cell cycle and Wnt/β-catenin, Hedgehog and Notch signaling, were upregulated compared to dexamethasone alone group.

Conclusions

LiCl has the potential to partially rescue from dexamethasone-induced bone growth impairment in an ex vivo model. Transcriptomics identified cell renewal and proliferation as candidates for the underlying mechanisms. Our observations may open up the development of a new treatment strategy for bone growth disorders.

Impact

LiCl is capable to prevent glucocorticoid-induced growth failure in rat metatarsals in vitro.

The accompanying drug-induced transcriptomic changes suggested cell renewal and proliferation as candidate underlying mechanisms.

Wnt/beta-catenin pathway could be one of those novel mechanisms.
Vitamin D opposes multilineage cell differentiation induced by Notch inhibition and BMP4 pathway activation in human colon organoids
Authors: Bustamante-Madrid Pilar , Barbáchano Antonio , Albandea-Rodríguez David , Rodríguez-Cobos Javier , Rodríguez-Salas Nuria , Prieto Isabel , Burgos Aurora , Martínez de Villarreal Jaime , Real Francisco X. , González-Sancho José Manuel , Larriba María Jesús , Lafarga Miguel , Muñoz Alberto , Fernández-Barral Asunción
Published: April 2024

Understanding the mechanisms involved in colonic epithelial differentiation is key to unraveling the alterations causing inflammatory conditions and cancer. Organoid cultures provide an unique tool to address these questions but studies are scarce. We report a differentiation system toward enterocytes and goblet cells, the two major colonic epithelial cell lineages, using colon organoids generated from healthy tissue of colorectal cancer patients. Culture of these organoids in medium lacking stemness agents resulted in a modest ultrastructural differentiation phenotype with low-level expression of enterocyte (KLF4, KRT20, CA1, FABP2) and goblet cell (TFF2, TFF3, AGR2) lineage markers. BMP pathway activation through depletion of Noggin and addition of BMP4 resulted in enterocyte-biased differentiation. Contrarily, blockade of the Notch pathway using the γ-secretase inhibitor dibenzazepine (DBZ) favored goblet cell differentiation. Combination treatment with BMP4 and DBZ caused a balanced strong induction of both lineages. In contrast, colon tumor organoids responded poorly to BMP4 showing only weak signals of cell differentiation, and were unresponsive to DBZ. We also investigated the effects of 1α,25-dihydroxyvitamin D3 (calcitriol) on differentiation. Calcitriol attenuated the effects of BMP4 and DBZ on colon normal organoids, with reduced expression of differentiation genes and phenotype. Consistently, in normal organoids, calcitriol inhibited early signaling by BMP4 as assessed by reduction of the level of phospho-SMAD1/5/8. Our results show that BMP and Notch signaling play key roles in human colon stem cell differentiation to the enterocytic and goblet cell lineages and that calcitriol modulates these processes favoring stemness features.
METTL3-mediated deficiency of lncRNA HAR1A drives non-small cell lung cancer growth and metastasis by promoting ANXA2 stabilization
Authors: Ling Xiaodong , Qi Cuicui , Cao Kui , Lu Mengdi , Yang Yingnan , Zhang Jinfeng , Zhang Luquan , Zhu Jinhong , Ma Jianqun
Published: April 2024

We previously reported lncRNA HAR1A as a tumor suppressor in non-small cell lung cancer (NSCLC). However, the delicate working mechanisms of this lncRNA remain obscure. Herein, we demonstrated that the ectopic expression of HAR1A inhibited the proliferation, epithelial-mesenchymal transition (EMT), migration, and invasion of NSCLC cells and enhanced paclitaxel (PTX) sensitivity in vitro and in vivo. We identified the oncogenic protein annexin 2 (ANXA2) as a potential interacting patterner of HAR1A. HAR1A overexpression enhanced ANXA2 ubiquitination and accelerated its degradation via the ubiquitin–proteasome pathway. We further uncovered that HAR1A promoted the interaction between E3 ubiquitin ligase TRIM65 and ANXA2. Moreover, the ANXA2 plasmid transfection could reverse HAR1A overexpression-induced decreases in proliferation, migration, and invasion of NSCLC cells and the activity of the NF-κB signaling pathway. Finally, we found that HAR1A loss in NSCLC might be attributed to the upregulated METTL3. The m6A modification levels of HAR1A were increased in cancer cells, while YTHDF2 was responsible for recognizing m6A modification in the HAR1A, leading to the disintegration of this lncRNA. In conclusion, we found that METTL3-mediated m6A modification decreased HAR1A in NSCLC. HAR1A deficiency, in turn, stimulated tumor growth and metastasis by activating the ANXA2/p65 axis.
Identifying therapeutic targets for cancer among 2074 circulating proteins and risk of nine cancers
Authors: Smith-Byrne Karl , Hedman Åsa , Dimitriou Marios , Desai Trishna , Sokolov Alexandr V. , Schioth Helgi B. , Koprulu Mine , Pietzner Maik , Langenberg Claudia , Atkins Joshua , Penha Ricardo Cortez , McKay James , Brennan Paul , Zhou Sirui , Richards Brent J. , Yarmolinsky James , Martin Richard M. , Borlido Joana , Mu Xinmeng J. , Butterworth Adam , Shen Xia , Wilson Jim , Assimes Themistocles L. , Hung Rayjean J. , Amos Christopher , Purdue Mark , Rothman Nathaniel , Chanock Stephen , Travis Ruth C. , Johansson Mattias , Mälarstig Anders
Published: April 2024

Circulating proteins can reveal key pathways to cancer and identify therapeutic targets for cancer prevention. We investigate 2,074 circulating proteins and risk of nine common cancers (bladder, breast, endometrium, head and neck, lung, ovary, pancreas, kidney, and malignant non-melanoma) using cis protein Mendelian randomisation and colocalization. We conduct additional analyses to identify adverse side-effects of altering risk proteins and map cancer risk proteins to drug targets. Here we find 40 proteins associated with common cancers, such as PLAUR and risk of breast cancer [odds ratio per standard deviation increment: 2.27, 1.88-2.74], and with high-mortality cancers, such as CTRB1 and pancreatic cancer [0.79, 0.73-0.85]. We also identify potential adverse effects of protein-altering interventions to reduce cancer risk, such as hypertension. Additionally, we report 18 proteins associated with cancer risk that map to existing drugs and 15 that are not currently under clinical investigation. In sum, we identify protein-cancer links that improve our understanding of cancer aetiology. We also demonstrate that the wider consequence of any protein-altering intervention on well-being and morbidity is required to interpret any utility of proteins as potential future targets for therapeutic prevention.
Loneliness trajectories over three decades are associated with conspiracist worldviews in midlife
Authors: Bierwiaczonek Kinga , Fluit Sam , von Soest Tilmann , Hornsey Matthew J. , Kunst Jonas R.
Published: April 2024

In the age of misinformation, conspiracy theories can have far-reaching consequences for individuals and society. Social and emotional experiences throughout the life course, such as loneliness, may be associated with a tendency to hold conspiracist worldviews. Here, we present results from a population-based sample of Norwegians followed for almost three decades, from adolescence into midlife (N = 2215). We examine participants’ life trajectories of loneliness using latent growth curve modeling. We show that people reporting high levels of loneliness in adolescence, and those who experience increasing loneliness over the life course, are more likely to endorse conspiracy worldviews in midlife.
HIV transmission dynamics and population-wide drug resistance in rural South Africa
Authors: Kemp Steven A. , Kamelian Kimia , Cuadros Diego F. ,   PANGEA Consortium,   Vukuzazi Team, Cheng Mark T. K. , Okango Elphas , Hanekom Willem , Ndung’u Thumbi , Pillay Deenan , Bonsall David , Wong Emily B. , Tanser Frank , Siedner Mark J. , Gupta Ravindra K.
Published: April 2024

Despite expanded antiretroviral therapy (ART) in South Africa, HIV-1 transmission persists. Integrase strand transfer inhibitors (INSTI) and long-acting injectables offer potential for superior viral suppression, but pre-existing drug resistance could threaten their effectiveness. In a community-based study in rural KwaZulu-Natal, prior to widespread INSTI usage, we enroled 18,025 individuals to characterise HIV-1 drug resistance and transmission networks to inform public health strategies. HIV testing and reflex viral load quantification were performed, with deep sequencing (20% variant threshold) used to detect resistance mutations. Phylogenetic and geospatial analyses characterised transmission clusters. One-third of participants were HIV-positive, with 21.7% having detectable viral loads; 62.1% of those with detectable viral loads were ART-naïve. Resistance to older reverse transcriptase (RT)-targeting drugs was found, but INSTI resistance remained low (<1%). Non-nucleoside reverse transcriptase inhibitor (NNRTI) resistance, particularly to rilpivirine (RPV) even in ART-naïve individuals, was concerning. Twenty percent of sequenced individuals belonged to transmission clusters, with geographic analysis highlighting higher clustering in peripheral and rural areas. Our findings suggest promise for INSTI-based strategies in this setting but underscore the need for RPV resistance screening before implementing long-acting cabotegravir (CAB) + RPV. The significant clustering emphasises the importance of geographically targeted interventions to effectively curb HIV-1 transmission.
Land conversion to agriculture induces taxonomic homogenization of soil microbial communities globally
Authors: Peng Ziheng , Qian Xun , Liu Yu , Li Xiaomeng , Gao Hang , An Yining , Qi Jiejun , Jiang Lan , Zhang Yiran , Chen Shi , Pan Haibo , Chen Beibei , Liang Chunling , van der Heijden Marcel G. A. , Wei Gehong , Jiao Shuo
Published: April 2024

Agriculture contributes to a decline in local species diversity and to above- and below-ground biotic homogenization. Here, we conduct a continental survey using 1185 soil samples and compare microbial communities from natural ecosystems (forest, grassland, and wetland) with converted agricultural land. We combine our continental survey results with a global meta-analysis of available sequencing data that cover more than 2400 samples across six continents. Our combined results demonstrate that land conversion to agricultural land results in taxonomic and functional homogenization of soil bacteria, mainly driven by the increase in the geographic ranges of taxa in croplands. We find that 20% of phylotypes are decreased and 23% are increased by land conversion, with croplands enriched inChloroflexi, Gemmatimonadota, Planctomycetota, Myxcoccota and Latescibacterota. Although there is no significant difference in functional composition between natural ecosystems and agricultural land, functional genes involved in nitrogen fixation, phosphorus mineralization and transportation are depleted in cropland. Our results provide a global insight into the consequences of land-use change on soil microbial taxonomic and functional diversity.
Antigen presentation plays positive roles in the regenerative response to cardiac injury in zebrafish
Authors: Cardeira-da-Silva João , Wang Qianchen , Sagvekar Pooja , Mintcheva Janita , Latting Stephan , Günther Stefan , Ramadass Radhan , Yekelchyk Michail , Preussner Jens , Looso Mario , Junker Jan Philipp , Stainier Didier Y. R.
Published: April 2024

In contrast to adult mammals, adult zebrafish can fully regenerate injured cardiac tissue, and this regeneration process requires an adequate and tightly controlled immune response. However, which components of the immune response are required during regeneration is unclear. Here, we report positive roles for the antigen presentation-adaptive immunity axis during zebrafish cardiac regeneration. We find that following the initial innate immune response, activated endocardial cells (EdCs), as well as immune cells, start expressing antigen presentation genes. We also observe that T helper cells, a.k.a. Cd4+T cells, lie in close physical proximity to these antigen-presenting EdCs. We targeted Major Histocompatibility Complex (MHC) class II antigen presentation by generatingcd74a; cd74bmutants, which display a defective immune response. In these mutants, Cd4+T cells and activated EdCs fail to efficiently populate the injured tissue and EdC proliferation is significantly decreased.cd74a; cd74bmutants exhibit additional defects in cardiac regeneration including reduced cardiomyocyte dedifferentiation and proliferation. Notably,Cd74also becomes activated in neonatal mouse EdCs following cardiac injury. Altogether, these findings point to positive roles for antigen presentation during cardiac regeneration, potentially involving interactions between activated EdCs, classical antigen-presenting cells, and Cd4+T cells.
Atypical heat shock transcription factor HSF5 is critical for male meiotic prophase under non-stress conditions
Authors: Yoshimura Saori , Shimada Ryuki , Kikuchi Koji , Kawagoe Soichiro , Abe Hironori , Iisaka Sakie , Fujimura Sayoko , Yasunaga Kei-ichiro , Usuki Shingo , Tani Naoki , Ohba Takashi , Kondoh Eiji , Saio Tomohide , Araki Kimi , Ishiguro Kei-ichiro
Published: April 2024

Meiotic prophase progression is differently regulated in males and females. In males, pachytene transition during meiotic prophase is accompanied by robust alteration in gene expression. However, how gene expression is regulated differently to ensure meiotic prophase completion in males remains elusive. Herein, we identify HSF5 as a male germ cell-specific heat shock transcription factor (HSF) for meiotic prophase progression. Genetic analyzes and single-cell RNA-sequencing demonstrate that HSF5 is essential for progression beyond the pachytene stage under non-stress conditions rather than heat stress. Chromatin binding analysis in vivo and DNA-binding assays in vitro suggest that HSF5 binds to promoters in a subset of genes associated with chromatin organization. HSF5 recognizes a DNA motif different from typical heat shock elements recognized by other canonical HSFs. This study suggests that HSF5 is an atypical HSF that is required for the gene expression program for pachytene transition during meiotic prophase in males.
Sulfur oxidation and reduction are coupled to nitrogen fixation in the roots of the salt marsh foundation plant Spartina alterniflora
Authors: Rolando J. L. , Kolton M. , Song T. , Liu Y. , Pinamang P. , Conrad R. , Morris J. T. , Konstantinidis K. T. , Kostka J. E.
Published: April 2024

Heterotrophic activity, primarily driven by sulfate-reducing prokaryotes, has traditionally been linked to nitrogen fixation in the root zone of coastal marine plants, leaving the role of chemolithoautotrophy in this process unexplored. Here, we show that sulfur oxidation coupled to nitrogen fixation is a previously overlooked process providing nitrogen to coastal marine macrophytes. In this study, we recovered 239 metagenome-assembled genomes from a salt marsh dominated by the foundation plant Spartina alterniflora, including diazotrophic sulfate-reducing and sulfur-oxidizing bacteria. Abundant sulfur-oxidizing bacteria encode and highly express genes for carbon fixation (RuBisCO), nitrogen fixation (nifHDK) and sulfur oxidation (oxidative-dsrAB), especially in roots stressed by sulfidic and reduced sediment conditions. Stressed roots exhibited the highest rates of nitrogen fixation and expression level of sulfur oxidation and sulfate reduction genes. Close relatives of marine symbionts from the Candidatus Thiodiazotropha genus contributed ~30% and ~20% of all sulfur-oxidizing dsrA and nitrogen-fixing nifK transcripts in stressed roots, respectively. Based on these findings, we propose that the symbiosis between S. alterniflora and sulfur-oxidizing bacteria is key to ecosystem functioning of coastal salt marshes.
Stress-shape misalignment in confluent cell layers
Authors: Nejad Mehrana R. , Ruske Liam J. , McCord Molly , Zhang Jun , Zhang Guanming , Notbohm Jacob , Yeomans Julia M.
Published: April 2024

In tissue formation and repair, the epithelium undergoes complex patterns of motion driven by the active forces produced by each cell. Although the principles governing how the forces evolve in time are not yet clear, it is often assumed that the contractile stresses within the cell layer align with the axis defined by the body of each cell. Here, we simultaneously measured the orientations of the cell shape and the cell-generated contractile stresses, observing correlated, dynamic domains in which the stresses were systematically misaligned with the cell body. We developed a continuum model that decouples the orientations of contractile stress and cell body. The model recovered the spatial and temporal dynamics of the regions of misalignment in the experiments. These findings reveal that the cell controls its contractile forces independently from its shape, suggesting that the physical rules relating cell forces and cell shape are more flexible than previously thought.
A global meta-analysis on the drivers of salt marsh planting success and implications for ecosystem services
Authors: Liu Zezheng , Fagherazzi Sergio , He Qiang , Gourgue Olivier , Bai Junhong , Liu Xinhui , Miao Chiyuan , Hu Zhan , Cui Baoshan
Published: April 2024

Planting has been widely adopted to battle the loss of salt marshes and to establish living shorelines. However, the drivers of success in salt marsh planting and their ecological effects are poorly understood at the global scale. Here, we assemble a global database, encompassing 22,074 observations reported in 210 studies, to examine the drivers and impacts of salt marsh planting. We show that, on average, 53% of plantings survived globally, and plant survival and growth can be enhanced by careful design of sites, species selection, and novel planted technologies. Planting enhances shoreline protection, primary productivity, soil carbon storage, biodiversity conservation and fishery production (effect sizes = 0.61, 1.55, 0.21, 0.10 and 1.01, respectively), compared with degraded wetlands. However, the ecosystem services of planted marshes, except for shoreline protection, have not yet fully recovered compared with natural wetlands (effect size = −0.25, 95% CI −0.29, −0.22). Fortunately, the levels of most ecological functions related to climate change mitigation and biodiversity increase with plantation age when compared with natural wetlands, and achieve equivalence to natural wetlands after 5–25 years. Overall, our results suggest that salt marsh planting could be used as a strategy to enhance shoreline protection, biodiversity conservation and carbon sequestration.
Snail-inspired robotic swarms: a hybrid connector drives collective adaptation in unstructured outdoor environments
Authors: Zhao Da , Luo Haobo , Tu Yuxiao , Meng Chongxi , Lam Tin Lun
Published: April 2024

Terrestrial self-reconfigurable robot swarms offer adaptable solutions for various tasks. However, most existing swarms are limited to controlled indoor settings, and often compromise stability due to their freeform connections. To address these issues, we present a snail robotic swarm system inspired by land snails, tailored for unstructured environments. Our system also employs a two-mode connection mechanism, drawing from the adhesive capabilities of land snails. The free mode, mirroring a snail’s natural locomotion, leverages magnet-embedded tracks for freeform mobility, thereby enhancing adaptability and efficiency. The strong mode, analogous to a snail’s response to disturbance, employs a vacuum sucker with directional polymer stalks for robust adhesion. By assigning specific functions to each mode, our system achieves a balance between mobility and secure connections. Outdoor experiments demonstrate the capabilities of individual robots and the exceptional synergy within the swarm. This research advances the real-world applications of terrestrial robotic swarms in unstructured environments.
Catalytic 1,1-diazidation of alkenes
Authors: Qiu Wangzhen , Liao Lihao , Xu Xinghua , Huang Hongtai , Xu Yang , Zhao Xiaodan
Published: April 2024

Compared to well-developed catalytic 1,2-diazidation of alkenes to produce vicinal diazides, the corresponding catalytic 1,1-diazidation of alkenes to yield geminal diazides has not been realized. Here we report an efficient approach for catalytic 1,1-diazidation of alkenes by redox-active selenium catalysis. Under mild conditions, electron-rich aryl alkenes with Z or E or Z/E mixed configuration can undergo migratory 1,1-diazidation to give a series of functionalized monoalkyl or dialkyl geminal diazides that are difficult to access by other methods. The method is also effective for the construction of polydiazides. The formed diazides are relatively safe by TGA-DSC analysis and impact sensitivity tests, and can be easily converted into various valuable molecules. In addition, interesting reactivity that geminal diazides give valuable molecules via the geminal diazidomethyl moiety as a formal leaving group in the presence of Lewis acid is disclosed. Mechanistic studies revealed that a selenenylation-deselenenylation followed by 1,2-aryl migration process is involved in the reactions, which provides a basis for the design of new reactions.
Gut microbiome composition and metabolic activity in women with diverticulitis
Authors: Ma Wenjie , Wang Yiqing , Nguyen Long H. , Mehta Raaj S. , Ha Jane , Bhosle Amrisha , Mclver Lauren J. , Song Mingyang , Clish Clary B. , Strate Lisa L. , Huttenhower Curtis , Chan Andrew T.
Published: April 2024

The etiopathogenesis of diverticulitis, among the most common gastrointestinal diagnoses, remains largely unknown. By leveraging stool collected within a large prospective cohort, we performed shotgun metagenomic sequencing and untargeted metabolomics profiling among 121 women diagnosed with diverticulitis requiring antibiotics or hospitalizations (cases), matched to 121 women without diverticulitis (controls) according to age and race. Overall microbial community structure and metabolomic profiles differed in diverticulitis cases compared to controls, including enrichment of pro-inflammatory Ruminococcus gnavus, 1,7-dimethyluric acid, and histidine-related metabolites, and depletion of butyrate-producing bacteria and anti-inflammatory ceramides. Through integrated multi-omic analysis, we detected covarying microbial and metabolic features, such as Bilophila wadsworthia and bile acids, specific to diverticulitis. Additionally, we observed that microbial composition modulated the protective association between a prudent fiber-rich diet and diverticulitis. Our findings offer insights into the perturbations in inflammation-related microbial and metabolic signatures associated with diverticulitis, supporting the potential of microbial-based diagnostics and therapeutic targets.
Mitochondrial complex I deficiency stratifies idiopathic Parkinson’s disease
Authors: Flønes Irene H. , Toker Lilah , Sandnes Dagny Ann , Castelli Martina , Mostafavi Sepideh , Lura Njål , Shadad Omnia , Fernandez-Vizarra Erika , Painous Cèlia , Pérez-Soriano Alexandra , Compta Yaroslau , Molina-Porcel Laura , Alves Guido , Tysnes Ole-Bjørn , Dölle Christian , Nido Gonzalo S. , Tzoulis Charalampos
Published: April 2024

Idiopathic Parkinson’s disease (iPD) is believed to have a heterogeneous pathophysiology, but molecular disease subtypes have not been identified. Here, we show that iPD can be stratified according to the severity of neuronal respiratory complex I (CI) deficiency, and identify two emerging disease subtypes with distinct molecular and clinical profiles. The CI deficient (CI-PD) subtype accounts for approximately a fourth of all cases, and is characterized by anatomically widespread neuronal CI deficiency, a distinct cell type-specific gene expression profile, increased load of neuronal mtDNA deletions, and a predilection for non-tremor dominant motor phenotypes. In contrast, the non-CI deficient (nCI-PD) subtype exhibits no evidence of mitochondrial impairment outside the dopaminergic substantia nigra and has a predilection for a tremor dominant phenotype. These findings constitute a step towards resolving the biological heterogeneity of iPD with implications for both mechanistic understanding and treatment strategies.
Highly efficient in crystallo energy transduction of light to work
Authors: Lin Jiawei , Zhou Jianmin , Li Liang , Tahir Ibrahim , Wu Songgu , Naumov Panče , Gong Junbo
Published: April 2024

Various mechanical effects have been reported with molecular materials, yet organic crystals capable of multiple dynamic effects are rare, and at present, their performance is worse than some of the common actuators. Here, we report a confluence of different mechanical effects across three polymorphs of an organic crystal that can efficiently convert light into work. Upon photodimerization, acicular crystals of polymorph I display output work densities of about 0.06–3.94 kJ m−3, comparable to ceramic piezoelectric actuators. Prismatic crystals of the same form exhibit very high work densities of about 1.5–28.5 kJ m−3, values that are comparable to thermal actuators. Moreover, while crystals of polymorph II roll under the same conditions, crystals of polymorph III are not photochemically reactive; however, they are mechanically flexible. The results demonstrate that multiple and possibly combined mechanical effects can be anticipated even for a simple organic crystal.
Serial founder effects slow range expansion in an invasive social insect
Authors: Hagan Thomas , Ding Guiling , Buchmann Gabriele , Oldroyd Benjamin P. , Gloag Rosalyn
Published: April 2024

Invasive populations often experience founder effects: a loss of genetic diversity relative to the source population, due to a small number of founders. Even where these founder effects do not impact colonization success, theory predicts they might affect the rate at which invasive populations expand. This is because secondary founder effects are generated at advancing population edges, further reducing local genetic diversity and elevating genetic load. We show that in an expanding invasive population of the Asian honey bee (Apis cerana), genetic diversity is indeed lowest at range edges, including at the complementary sex determiner, csd, a locus that is homozygous-lethal. Consistent with lower local csd diversity, range edge colonies had lower brood viability than colonies in the range centre. Further, simulations of a newly-founded and expanding honey bee population corroborate the spatial patterns in mean colony fitness observed in our empirical data and show that such genetic load at range edges will slow the rate of population expansion.
Parallel wavelength-division-multiplexed signal transmission and dispersion compensation enabled by soliton microcombs and microrings
Authors: Liu Yuanbin , Zhang Hongyi , Liu Jiacheng , Lu Liangjun , Du Jiangbing , Li Yu , He Zuyuan , Chen Jianping , Zhou Linjie , Poon Andrew W.
Published: April 2024

The proliferation of computation-intensive technologies has led to a significant rise in the number of datacenters, posing challenges for high-speed and power-efficient datacenter interconnects (DCIs). Although inter-DCIs based on intensity modulation and direct detection (IM-DD) along with wavelength-division multiplexing technologies exhibit power-efficient and large-capacity properties, the requirement of multiple laser sources leads to high costs and limited scalability, and the chromatic dispersion (CD) restricts the transmission length of optical signals. Here we propose a scalable on-chip parallel IM-DD data transmission system enabled by a single-soliton Kerr microcomb and a reconfigurable microring resonator-based CD compensator. We experimentally demonstrate an aggregate line rate of 1.68 Tbit/s over a 20-km-long SMF. The extrapolated energy consumption for CD compensation of 40-km-SMFs is ~0.3 pJ/bit, which is calculated as being around 6 times less than that of the commercial 400G-ZR coherent transceivers. Our approach holds significant promise for achieving data rates exceeding 10 terabits.