bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential for CO2 Reduction by Dynamic Wireless Power Transfer for Passenger Vehicles in Japan
Authors: Shimizu Osamu , Nagai Sakahisa , Fujita Toshiyuki , Fujimoto Hiroshi
Published: June 2020

In this study, a novel system named the third-generation wireless in-wheel motor (WIWM-3), which has a dynamic wireless power transfer (DWPT) system, is developed. It can extend the cruise range, which is one of the key specifications of electric vehicles. DWPT also reduces CO2 emission as the driving resistance is reduced due to light weight of the batteries. In this study, CO2 emission by an internal combustion vehicle, a long range drivable electric vehicle with the same cruise range, and an electric vehicle with WIWM-3 equipped with the DWPT system are analyzed using actual measurement data and calculated data based on actual measurement or specification data. A WPT system with WIWM-3 achieves 92.5% DC-to-DC efficiency as indicated by an actual measurement at the nominal position. Thus, the electric vehicle with DWPT can reduce up to 62% of CO2 emission in internal combustion vehicles, and the long-range drivable vehicle emits 17% more CO2 than the electric vehicle with DWPT. Moreover, it is expected that by 2050, electric vehicles with DWPT will reduce CO2 emissions from internal combustion vehicles by 95% in Japan. DWPT systems make electric vehicles more sustainable and, hence, more acceptable for consumers.
A Control Scheme with the Variable-Speed Pitch System for Wind Turbines during a Zero-Voltage Ride Through
Authors: Cai Enyu , Yan Yunqiang , Dong Lei , Liao Xiaozhong
Published: June 2020

Zero-voltage ride through (ZVRT) is the extreme case of low-voltage ride through (LVRT), which represents the optimal grid-connection capability of wind turbines (WTs). Enforcing ZVRT will improve the dynamic performance of WTs and therefore significantly enhance the resiliency of renewable-rich grids. A control scheme that includes a pitch system is an essential control aspect of WTs riding through voltage dips; however, the existing control scheme with a pitch system for LVRT cannot distinguish between a ZVRT status and a power-loss condition, and, consequently, does not meet the ZVRT requirements. A system-level control scheme with a pitch system for ZVRT that includes pitch system modeling, control logic, control circuits, and overspeed protection control (OPC) is proposed in this paper for the first time in ZVRT research. Additionally, the field data are shared, a fault analysis of an overspeed accident caused by a voltage dip that describes the operating status at the WT-collapse moment is presented, and some existing WT design flaws are revealed and corrected by the fault analysis. Finally, the pitching performance during a ZVRT, which significantly affects the ZVRT performance of the WT, is obtained from laboratory and field tests. The results validate the effectiveness of the proposed holistic control scheme.
Evaluation of the Hot Air Recirculation Effect and Relevant Empirical Formulae Applicability for Mechanical Draft Wet Cooling Towers
Authors: Dong Haotian , Wan Dawei , Liu Minghua , Chen Tiefeng , Gao Shasha , Zhao Yuanbin
Published: June 2020

Due to the hot air recirculation, the inlet air enthalpy h1 of mechanical draft wet cooling towers (MCTs) was usually greater than the ambient air enthalpy ha. To realize the cooling performance and accurate design of MCTs, this paper clarified the feasibility of the inlet air enthalpy empirical formula presented by the Cooling Technology Institute (CTI) of the USA. A three-dimensional (3D) numerical model was established for a representative power plant, with full consideration of MCTs and adjacent main workshops, which were validated by design data and published test results. By numerical simulation, the influence of different wind directions and wind speeds on hot air recirculation (HAR) and the influence of HAR on the cooling performance of the MCTs were qualitatively studied based on the concept of hot air recirculation rate (HRR), and the correction value of HRR was compared with the calculated value of the CTI standard. The evaluation coefficient ηh, representing the ratio of the corrected value to the calculated value was introduced to evaluate the applicability of the CTI formula. It was found that HAR was more sensitive to ambient crosswind, and an increase in HRR would deteriorate the tower cooling performance. When the crosswind speed increased from 0 to 15 m/s, ηh, changed from 2.42 to 80.18, and the calculation error increased accordingly. It can be concluded that the CTI empirical HRR formula should be corrected when there are large buildings around the MCTs, especially under high-speed ambient crosswind conditions.
A Drone Based Transmission Line Components Inspection System with Deep Learning Technique
Authors: Siddiqui Zahid Ali , Park Unsang
Published: June 2020

Defects in high voltage transmission line components such as cracked insulators, broken wires rope, and corroded power line joints, are very common due to continuous exposure of these components to harsh environmental conditions. Consequently, they pose a great threat to humans and the environment. This paper presents a real-time aerial power line inspection system that aims to detect power line components such as insulators (polymer and porcelain), splitters, damper-weights, power lines, and then analyze these transmission line components for potential defects. The proposed system employs a deep learning-based framework using Jetson TX2 embedded platform for the real-time detection and localization of these components from a live video captured by remote-controlled drone. The detected components are then analyzed using novel defect detection algorithms, presented in this paper. Results show that the proposed detection and localization system is robust against highly cluttered environment, while the proposed defect analyzer outperforms similar researches in terms of defect detection precision and recall. With the help of the proposed system automatic defect analyzing system, manual inspection time can be reduced.
Energy Management Strategies for Hybrid Electric Vehicles: Review, Classification, Comparison, and Outlook
Authors: Zhang Fengqi , Wang Lihua , Coskun Serdar , Pang Hui , Cui Yahui , Xi Junqiang
Published: June 2020

Hybrid Electric Vehicles (HEVs) have been proven to be a promising solution to environmental pollution and fuel savings. The benefit of the solution is generally realized as the amount of fuel consumption saved, which by itself represents a challenge to develop the right energy management strategies (EMSs) for HEVs. Moreover, meeting the design requirements are essential for optimal power distribution at the price of conflicting objectives. To this end, a significant number of EMSs have been proposed in the literature, which require a categorization method to better classify the design and control contributions, with an emphasis on fuel economy, providing power demand, and real-time applicability. The presented review targets two main headlines: (a) offline EMSs wherein global optimization-based EMSs and rule-based EMSs are presented; and (b) online EMSs, under which instantaneous optimization-based EMSs, predictive EMSs, and learning-based EMSs are put forward. Numerous methods are introduced, given the main focus on the presented scheme, and the basic principle of each approach is elaborated and compared along with its advantages and disadvantages in all aspects. In this sequel, a comprehensive literature review is provided. Finally, research gaps requiring more attention are identified and future important trends are discussed from different perspectives. The main contributions of this work are twofold. Firstly, state-of-the-art methods are introduced under a unified framework for the first time, with an extensive overview of existing EMSs for HEVs. Secondly, this paper aims to guide researchers and scholars to better choose the right EMS method to fill in the gaps for the development of future-generation HEVs.
A Novel Analytical Wake Model with a Cosine-Shaped Velocity Deficit
Authors: Zhang Ziyu , Huang Peng , Sun Haocheng
Published: June 2020

A novel analytical model is proposed and validated in this paper to predict the velocity deficit in the wake downwind of a wind turbine. The model is derived by employing mass and momentum conservation and assuming a cosine-shaped distribution for the velocity deficit. In this model, a modified wake growth rate rather than a constant one is chosen to take into account the effects of the ambient turbulence and the mechanical turbulence generated. The model was tested against field observations, wind-tunnel measurements in different thrust operations and high-resolution large-eddy simulations (LES) for two aerodynamic roughness lengths. It was found that the normalized velocity deficit predicted by the proposed model shows good agreement with experimental and numerical data in terms of shape and magnitude in the far wake region ( x / d 0 > 3 ). Based on the proposed model, predictions from multiple views and at different locations are demonstrated to show the spatial distribution of streamwise velocity downwind of a wind turbine. The result shows that the model is suitable for predicting streamwise velocity fields and thus could provide some references for the selection of wind turbine spacing.
Comparative Analysis of the Nutritional Components and Antioxidant Activities of Different Brassica juncea Cultivars
Authors: Kwon Hee-Yeon , Choi Sun-Il , Park Hye-In , Choi Seung-Hyun , Sim Wan-Sup , Yeo Jin-Hui , Cho Ju-Hyun , Lee Ok-Hwan
Published: June 2020

The purpose of this study was to compare the nutritional components and antioxidant activities of two different cultivars of Brassica juncea (Dolsan, Yeosu, Korea (BJD) and (Jeongseon, Gangwon, Korea (BJJ)). We investigated the proximate composition (moisture, crude ash, crude protein and crude lipid), antioxidant activities (2,2-Diphenyl-2-picrylhydrazil (DPPH) scavenging activity and ferric reducing antioxidant power (FRAP)), total phenol content, total flavonoid content and sinigrin content by high-performance liquid chromatography analysis. Our results show that the proximate compositions of BJD and BJJ were not significantly different. However, both the DPPH radical scavenging and FRAP activities of the BJJ extracts were higher than those of the BJD extracts. The total phenol contents of the BJD and BJJ extracts were 6.56 and 9.80 mg gallic acid equivalent/g, respectively. The total flavonoids content of the BJD and BJJ extracts were 20.92 and 34.81 mg rutin equivalent/g, respectively, whereas the sinigrin contents, one of the major compounds in BJD and BJJ extracts, were 16.16 mg/g and 11.73 mg/g, respectively. In this study, we confirmed that, by comparing BJJ and BJD, the sinigrin content of BJD was higher than that of BJJ, but the antioxidant activity and phenol content of BJD were superior to that of BJJ.
Immunohistochemical Study of Glucose Transporter GLUT-5 in Duodenal Epithelium in Norm and in T-2 Mycotoxicosis
Authors: Hussar Piret , Popovska-Percinic Florina , Blagoevska Katerina , Järveots Tõnu , Dūrītis Ilmārs
Published: June 2020

Although patterns of glucose transporter expression and notes about diseases leading to adaptive changes in intestinal fructose transport have been well-characterized, the connection between infection and fructose transportation has been lightly investigated. Up to now only few studies on GLUT-5 expression and function under pathological conditions in bird intestines have been carried out. The aim of our current research was to immunolocalize GLUT-5 in chicken duodenal epithelium in norm and during T-2 mycotoxicosis. Material from chicken (Gallus gallus domesticus) duodenum was collected from twelve seven-day-old female broilers, divided into control group and broilers with T-2 mycotoxicosis. The material was fixed with 10% formalin and thereafter embedded into paraffin; slices 7 μm in thickness were cut, followed by immunohistochemical staining, according to the manufacturers guidelines (IHC kit, Abcam, UK) using polyclonal primary antibody Rabbit anti-GLUT-5. Our study revealed the strong expression of GLUT-5 in the apical parts of the duodenal epithelial cells in the control group chickens and weak staining for GLUT-5 in the intestinal epithelium in the T-2 mycotoxicosis group. Our results confirmed decreased the expression of GLUT-5 in the duodenal epithelium during T-2 mycotoxicosis.
Identification and Quantification of Adulterants in Coffee (Coffea arabica L.) Using FT-MIR Spectroscopy Coupled with Chemometrics
Authors: Flores-Valdez Mauricio , Meza-Márquez Ofelia Gabriela , Osorio-Revilla Guillermo , Gallardo-Velázquez Tzayhri
Published: June 2020

Food adulteration is an illegal practice performed to elicit economic benefits. In the context of roasted and ground coffee, legumes, cereals, nuts and other vegetables are often used to augment the production volume; however, these adulterants lack the most important coffee compound, caffeine, which has health benefits. In this study, the mid-infrared Fourier transform spectroscopy (FT-MIR) technique coupled with chemometrics was used to identify and quantify adulterants in coffee (Coffea arabica L.). Coffee samples were adulterated with corn, barley, soy, oat, rice and coffee husks, in proportions ranging from 1–30%. A discrimination model was developed using the soft independent modeling of class analogy (SIMCA) framework, and quantitative models were developed using such algorithms as the partial least squares algorithms with one variable (PLS1) and multiple variables (PLS2) and principal component regression (PCR). The SIMCA model exhibited an accuracy of 100% and could discriminate among all the classes. The quantitative model with the highest performance corresponded to the PLS1 algorithm. The model exhibited an R2c: ≥ 0.99, standard error of calibration (SEC) of 0.39–0.82, and standard error of prediction (SEP) of 0.45–0.94. The developed models could identify and quantify the coffee adulterants, and it was considered that the proposed methodology can be applied to identify and quantify the adulterants used in the coffee industry.
Feeding, Muscle and Packaging Effects on Meat Quality and Consumer Acceptability of Avileña-Negra Ibérica Beef
Authors: Barahona Marta , Hachemi Mohamed Amine , Olleta José Luis , González María del Mar , Campo María del Mar
Published: June 2020

In order to achieve an attractive and differentiated product for the consumer and to optimize and to maximize profitability for the farmers within the EU Protected Geographical Indication “Carne de Ávila”, 24 yearling males of Avileña-Negra Ibérica breed were used to evaluate the effect of 2 feeding systems, concentrate (CON) and maize silage (SIL), and 2 packaging systems, vacuum (VAC) and modified atmosphere (MAP), on fatty acid composition, proximate analysis, water holding capacity and consumer acceptability of meat in 2 muscles: Longissimus thoracis (LT) and Semitendinosus (ST). Animals fed with concentrate showed higher carcass weight. However, feeding did not affect the proximate analysis of the meat. The use of maize silage improved the amount of conjugated linoleic acid and n-3 polyunsaturated fatty acids (PUFA) and the relation n-6 PUFA/n-3 PUFA. In LT muscle, feeding influenced texture, samples from SIL being more tender. The VAC packaging showed higher cooking losses than MAP in both muscles. Aging increased tenderness and cooking losses but decreased thawing losses. LT samples from SIL feeding were better accepted by consumers and VAC packaging showed higher scores than MAP. We can conclude that the use of maize silage could be an alternative feeding for this type of animals improving some aspects of the quality of the meat.
Perceived Academic Self-Efficacy among Romanian Upper Secondary Education Students
Authors: Zamfir Ana-Maria , Mocanu Cristina
Published: June 2020

Perceived academic self-efficacy represents an important component of students’ mental health and well-being. The link between efficacy beliefs and level of academic performances relies on the fact that they regulate the anxiety and foster motivation, school engagement, effort, and perseverance of students. This paper aims to identify factors that are conducive for more efficacious beliefs in different socio-economic and educational contexts. We build our analysis on data collected from a sample of Romanian upper secondary education students on their beliefs in relation to their ability to perform at the baccalaureate exam. We employ decision tree models in order to unveil the way factors interact and predict perceived academic self-efficacy, with focus on the positive support received from parents and teachers, as well as on features of the school environment. Our results can be useful for building more resilient educational environments that support mental health and academic well-being of students.
Mindfulness-Based Interventions for People with Schizophrenia: A Systematic Review and Meta-Analysis
Authors: Hodann-Caudevilla Ricardo M. , Díaz-Silveira Cintia , Burgos-Julián Francisco A. , Santed Miguel A.
Published: June 2020

(1) Background: There is increasing interest in the practice of mindfulness-based interventions (MBIs) to treat people with schizophrenia, as evidenced by the publication of different randomized controlled trials (RCTs). However, no meta-analysis of RCTs has been carried out to date with the exclusive inclusion of this type of interventions. (2) Objective: To analyze empirical evidence regarding the effectiveness of MBIs for the improvement of clinical parameters associated with schizophrenia. Method: A systematic review and meta-analysis was conducted of RCTs published in the databases PsycINFO, PubMed, WOS, and Cochrane Library. (3) Results: A total of 10 articles (n = 1094) fulfilled the criteria for inclusion in the review. The analysis of these studies suggests that MBIs combined with standard interventions are able to generate significant improvements in a variety of clinical schizophrenia-related parameters, such as the intensity of overall symptomatology (g = 0.72), positive symptoms (g = 0.32), negative symptoms (g = 0.40), functioning level (g = 1.28), and awareness of illness (g = 0.65). (4) Conclusions: There is evidence that supports the effectiveness and safety of MBIs for the treatment of people with schizophrenia. The results obtained by MBIs are comparable to those obtained by cognitive-behavioral therapy for psychosis. However, given the heterogeneity of the applied interventions and the methodological limitations found in the reviewed trials, the results should be interpreted with caution.
Modeling and Forecasting the COVID-19 Temporal Spread in Greece: An Exploratory Approach Based on Complex Network Defined Splines
Authors: Demertzis Konstantinos , Tsiotas Dimitrios , Magafas Lykourgos
Published: June 2020

Within the complex framework of anti-COVID-19 health management, where the criteria of diagnostic testing, the availability of public-health resources and services, and the applied anti-COVID-19 policies vary between countries, the reliability and accuracy in the modeling of temporal spread can prove to be effective in the worldwide fight against the disease. This paper applies an exploratory time-series analysis to the evolution of the disease in Greece, which currently suggests a success story of COVID-19 management. The proposed method builds on a recent conceptualization of detecting connective communities in a time-series and develops a novel spline regression model where the knot vector is determined by the community detection in the complex network. Overall, the study contributes to the COVID-19 research by proposing a free of disconnected past-data and reliable framework of forecasting, which can facilitate decision-making and management of the available health resources.
The Attitudes and Professional Approaches of Dental Practitioners during the COVID-19 Outbreak in Poland: A Cross-Sectional Survey
Authors: Tysiąc-Miśta Monika , Dziedzic Arkadiusz
Published: June 2020

The coronavirus infectious disease 2019 (COVID-19) pandemic has put enormous pressure on health care systems around the world. Dentistry has had to adjust to the new epidemic situation to not only bring relief to suffering patients but also to avoid becoming a source of SARS-CoV-2 transmission. Methods: A comprehensive, cross-sectional survey was conducted between April 6 and 16, 2020 among 875 Polish dental practitioners. The aim of the research was to assess dentists’ attitudes and professional approaches resulting from the COVID-19 pandemic. Results: 71.2% of dentists who responded to the questionnaire decided to suspend their clinical practice during that particular time. The main factors for this fact were the shortage of personal protective equipment (PPE), the respondents’ subjective perceptions of the risk of COVID-19 contraction and a general feeling of anxiety and uncertainty regarding the COVID-19 situation. The authors observed a significant decrease in the number of patients admitted weekly in April 2020 (12.06; SD, 11.55) in comparison to that in the time before the state of pandemic was declared on March 11, 2020 (49.21; SD, 24.97). Conclusions: Due to the unpreparedness of the dental sector, both in national health and private settings, most of the Polish dentists decided to voluntarily suspend their clinical practice in order to mitigate the spread of the disease. The COVID-19 outbreak has revealed numerous shortcomings in the dental care system, especially regarding the insufficient coordination of health services related to the pandemic and lack of advanced PPE. This has led to an overwhelming feeling of fear, confusion and anxiety among dental professionals in Poland and a sudden decrease in the number of performed dental procedures. Hopefully enriched with the recent experience and due to the implementation of proper strategic and long-term measures, dental practitioners will be better prepared and adapted to global health care disruptions in the future.
Deconstructing Syndemics: The Many Layers of Clustering Multi-Comorbidities in People Living with HIV
Authors: Peprah Emmanuel , Caler Elisabet , Snyder Anya , Ketema Fassil
Published: June 2020

The HIV epidemic has dramatically changed over the past 30 years; there are now fewer newly infected people (especially children), fewer AIDS-related deaths, and more people with HIV (PWH) receiving treatment. However, the HIV epidemic is far from over. Despite the tremendous advances in anti-retroviral therapies (ART) and the implementation of ART regimens, HIV incidence (number of new infections over a defined period of time) and prevalence (the burden of HIV infection) in certain regions of the world and socio-economic groups are still on the rise. HIV continues to disproportionally affect highly marginalized populations that constitute higher-risk and stigmatized groups, underserved and/or neglected populations. In addition, it is not uncommon for PWH to suffer enhanced debilitating conditions resulting from the synergistic interactions of both communicable diseases (CDs) and non-communicable diseases (NCDs). While research utilizing only a comorbidities framework has advanced our understanding of the biological settings of the co-occurring conditions from a molecular and mechanistic view, harmful interactions between comorbidities are often overlooked, particularly under adverse socio-economical and behavioral circumstances, likely prompting disease clustering in PWH. Synergistic epidemics (syndemics) research aims to capture these understudied interactions: the mainly non-biological aspects that are central to interpret disease clustering in the comorbidities/multi-morbidities only framework. Connecting population-level clustering of social and health problems through syndemic interventions has proved to be a critical knowledge gap that will need to be addressed in order to improve prevention and care strategies and bring us a step closer to ending the HIV epidemic.
Causes and Phenotypes of Work-Related Asthma
Authors: Maestrelli Piero , Henneberger Paul K. , Tarlo Susan , Mason Paola , Boschetto Piera
Published: June 2020

Work-related asthma (WRA) includes heterogeneous conditions, which have in common (i) symptoms and signs compatible with asthma and (ii) a relationship with exposures in the workplace. The types of WRA described in this review are distinguished by their etiology, comprising of work-exacerbated asthma (WEA), irritant-induced asthma (IIA), and immunologic occupational asthma (OA). There have been significant advances in the definition and characterization of the different forms of WRA by international panels of experts. The present review provides a comprehensive and updated view of the current knowledge on causes and phenotypes of WRA. Health care practitioners should consider WRA in any case of adult asthma, given that one fifth of workers with asthma report symptoms of WEA and it has been estimated that OA represents 10% to 25% of asthma in adulthood. The information provided in this review will facilitate the physician in the recognition of the different forms of WRA, since it has been established that five categories of agents are responsible for at least 60% of WEA cases and seven groups of agents are the cause of 70% of immunologic OA. In addition, there is agreement that IIA can be elicited not only by a single massive irritant exposure, but also by low/moderate repeated irritant exposures.
Cumulative Exposure to Adverse Childhood Experience: Depressive Symptoms, Suicide Intensions and Suicide Plans among Senior High School Students in Nanchang City of China
Authors: Jia Zhihui , Wen Xiaotong , Chen Feiyu , Zhu Hui , Li Can , Lin Yixiang , Xie Xiaoxu , Yuan Zhaokang
Published: June 2020

This study tested relationships between different types of adverse childhood experiences (ACE) and depressive symptoms, suicide intensions, suicide plans and examines the cumulative effects of adverse childhood experience on depressive, suicide intentions and suicide plans among senior high school students. We conducted a survey among five senior high schools in Nanchang city, which were selected through stratified random cluster sampling. Among the 884 respondents, 409 were male (46.27%), and 475 were female (53.73%); the age ranged from 14 to 18. During the past 12 months, 199 (22.51%) students presented to depressive symptoms, 125 (14.14%) students had suicide intensions, 55 (6.22%) students had suicide plans. As ACE scores increased, there was an increase in the odds of (1) depressive symptoms—one ACE (adjusted odds ratio, AOR = 2.096, p < 0.001), two ACEs (AOR = 3.155, p < 0.001) and three to five ACEs (AOR = 9.707, p < 0.001); suicide intensions-1 ACE (AOR = 1.831, p = 0.011), two ACEs (AOR = 2.632, p = 0.002) and three to five ACEs (AOR = 10.836, p < 0.001); and (2) suicide plans—one ACE (AOR = 2.599, p < 0.001), two ACEs (AOR = 4.748, p < 0.001) and three to five ACEs (AOR = 22.660, p < 0.001). We should increase the awareness of adolescents who have had adverse childhood experience, especially those with multiple ACEs to prevent depression and suicide among senior high school students.
Monitoring of the Organophosphate Pesticide Chlorpyrifos in Vegetable Samples from Local Markets in Northern Thailand by Developed Immunoassay
Authors: Hongsibsong Surat , Prapamontol Tippawan , Xu Ting , Hammock Bruce D. , Wang Hong , Chen Zi-Jian , Xu Zhen-Lin
Published: June 2020

Chlorpyrifos is an organophosphate pesticide that is wildly used among farmers for crop protection. However, there are concerns regarding its contamination in the environment and food chain. In the present study, an in-house indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) specific for detecting chlorpyrifos is developed and validated against gas chromatography–flame photometric detection (GC-FPD) as the conventional method. The developed ic-ELISA was used for detecting chlorpyrifos residue in vegetable samples. The developed ic-ELISA showed good sensitivity to chlorpyrifos at an IC50 of 0.80 µg/kg, with low cross-reactivity to other organophosphate pesticides. The 160 samples were collected from local markets located in the Chiang Rai, Chiang Mai, and Nan provinces in northern Thailand. The positive rate of chlorpyrifos residues in the vegetable samples was 33.8%, with the highest levels found in cucumbers, coriander, and morning glory, at 275, 145, and 35.3 µg/kg, respectively. The highest median levels of chlorpyrifos found in the detected samples were Chinese cabbage (332 μg/kg), cucumber (146.3 μg/kg) and Chinese Kale (26.95 μg/kg). The developed ic-ELISA is suitable for the rapid quantitation of chlorpyrifos residues.
Research on the Colors of Military Symbols in Digital Situation Maps Based on Event-Related Potential Technology
Authors: Niu Yafeng , Ma Guorui , Xue Wei , Xue Chengqi , Zhou Tianyu , Gao Yue , Zuo Hongrui , Jin Tao
Published: June 2020

Under the trend of increasingly informationalized military operations and the increasing maneuverability of combat units, military commanders have put forward higher requirements for the accuracy and promptness of information on battlefield situation maps. Based on the sea battlefield, this paper studies the pros and cons of the color matching of military symbols on sea situation maps. Fifteen colors, where each Hue had five colors, were chosen using the Munsell Color System according to Chroma axis and the Value axis on a span of 2 and 4. By collecting and analyzing the P300 EEG data, reaction time data, and accuracy data of 20 subjects, a better color matching selection of military symbols on pure color (L = 85, a = −10, and b = −23) sea situation maps is put forward, and the conclusions are as follows: (1) the different colors all cause the P300 component in EEG experiment. Among them, the P300 amplitude that is caused by military symbols with lower Chroma is smaller and the latency is shorter, indicating that the user experience and efficiency of low Chroma color symbols will be better than those with high Chroma color symbols. (2) High Value color map military symbols cause higher P300 amplitude and longer latency. According to the results above, this paper puts forward three optimized colors, namely, blue (L = 39, a = 20, and b = −49), green (L = 80, a = −72, and b = 72), and red (L = 20, a = 41, and b = 28). Additionally, three map interfaces were designed to confirm the validity of these colors. By means of applying the NASA-TLX (Task Load Index) scale to evaluate the task load of the confirmation interfaces, it can be concluded that these three optimized colors are preferred by users who are skilled in GIS and interface design. Therefore, the research conclusion of this paper can provide important reference values for military map design, which is helpful in shortening the identification and judgment time during the use of situation maps and it can improve users’ operation performance.
Spatial Exploration of Economic Data—Insight into Attitudes of Students towards Interdisciplinary Knowledge
Authors: Sternad Zabukovšek Simona , Tominc Polona , Bobek Samo , Štrukelj Tjaša
Published: June 2020

This paper uses the technology acceptance model (TAM) framework for the research of economic and geography students’ attitudes towards interdisciplinary knowledge. Based on the SmartPLS Structural equation modelling SEM variance-based method, research results were gained through analysis of survey data of economic and geography students. They participated in the Spationomy project in the period of 2017–2019. Online questionnaires were fulfilled before and after students’ participation in the project and their future behavioural intention to use interdisciplinary knowledge was analysed. Based on the research, we can confirm that the Spationomy project has achieved its purpose, as both groups of students (economic and geography students) have acquired interdisciplinary knowledge and students intend to use it also in the future. Therefore, we can argue that the students included in the project in practice gained recognition of systems thinking about the importance of mutual interdisciplinary cooperation towards achieving synergies. The results also show that TAM can be successfully implemented to analyse how students of economics and geography accept the use of interdisciplinary knowledge in the learning process, which is an important implication for management and education as well as from the theoretical implications viewpoint. While effective analysis using TAM has been used successfully and relatively frequently in economics and business field, we have not found relevant examples of its implementation in the broader field of geography. However, the acceptance of geographic information system (GIS) or other information technologies/information software (IT/IS) tool-based approaches of analysis in the geography field may be of most importance. Therefore, also, this represents an important implication for the research area.
The Abuse Potential of Novel Synthetic Phencyclidine Derivative 1-(1-(4-Fluorophenyl)Cyclohexyl)Piperidine (4′-F-PCP) in Rodents
Authors: Ryu In Soo , Kim Oc-Hee , Lee Young Eun , Kim Ji Sun , Li Zhan-Hui , Kim Tae Wan , Lim Ri-Na , Lee Young Ju , Cheong Jae Hoon , Kim Hee Jin , Lee Yong Sup , Steffensen Scott C. , Lee Bong Hyo , Seo Joung-Wook , Jang Eun Young
Published: June 2020

The dissociative anesthetic phencyclidine (PCP) and PCP derivatives, including 4′-F-PCP, are illegally sold and abused worldwide for recreational and non-medical uses. The psychopharmacological properties and abuse potential of 4′-F-PCP have not been fully characterized. In this study, we evaluated the psychomotor, rewarding, and reinforcing properties of 4′-F-PCP using the open-field test, conditioned place preference (CPP), and self-administration paradigms in rodents. Using Western immunoblotting, we also investigated the expression of dopamine (DA)-related proteins and DA-receptor-mediated downstream signaling cascades in the nucleus accumbens (NAc) of 4′-F-PCP-self-administering rats. Intraperitoneal administration of 10 mg/kg 4′-F-PCP significantly increased locomotor and rearing activities and increased CPP in mice. Intravenous administration of 1.0 mg/kg/infusion of 4′-F-PCP significantly enhanced self-administration during a 2 h session under fixed ratio schedules, showed a higher breakpoint during a 6 h session under progressive ratio schedules of reinforcement, and significantly altered the expression of DA transporter and DA D1 receptor in the NAc of rats self-administering 1.0 mg/kg 4′-F-PCP. Additionally, the expression of phosphorylated (p) ERK, pCREB, c-Fos, and FosB/ΔFosB in the NAc was significantly enhanced by 1.0 mg/kg 4′-F-PCP self-administration. Taken together, these findings suggest that 4′-F-PCP has a high potential for abuse, given its robust psychomotor, rewarding, and reinforcing properties via activation of DAergic neurotransmission and the downstream signaling pathways in the NAc.
Skeletal Muscle and the Effects of Ammonia Toxicity in Fish, Mammalian, and Avian Species: A Comparative Review Based on Molecular Research
Authors: Miramontes Emily , Mozdziak Paul , N. Petitte James , Kulus Magdalena , Wieczorkiewicz Maria , Kempisty Bartosz
Published: June 2020

Typically, mammalian and avian models have been used to examine the effects of ammonia on skeletal muscle. Hyperammonemia causes sarcopenia or muscle wasting, in mammals and has been linked to sarcopenia in liver disease patients. Avian models of skeletal muscle have responded positively to hyperammonemia, differing from the mammalian response. Fish skeletal muscle has not been examined as extensively as mammalian and avian muscle. Fish skeletal muscle shares similarities with avian and mammalian muscle but has notable differences in growth, fiber distribution, and response to the environment. The wide array of body sizes and locomotion needs of fish also leads to greater diversity in muscle fiber distribution and growth between different fish species. The response of fish muscle to high levels of ammonia is important for aquaculture and quality food production but has not been extensively studied to date. Understanding the differences between fish, mammalian and avian species’ myogenic response to hyperammonemia could lead to new therapies for muscle wasting due to a greater understanding of the mechanisms behind skeletal muscle regulation and how ammonia effects these mechanisms. This paper provides an overview of fish skeletal muscle and ammonia excretion and toxicity in fish, as well as a comparison to avian and mammalian species.
A Simple Method for the Production of Human Skin Equivalent in 3D, Multi-Cell Culture
Authors: Szymański Łukasz , Jęderka Krystyna , Cios Aleksandra , Ciepelak Martyna , Lewicka Aneta , Stankiewicz Wanda , Lewicki Sławomir
Published: June 2020

An important problem for researchers working in the field of dermatology is the preparation of the human skin equivalent (HSE). Here, we describe a simple and reliable protocol for preparing a skin model from the commercially available cell lines: keratinocytes, fibroblasts, and melanocytes. Importantly, in our 3D model, the keratinocytes are diverse that brings this model closer to the natural skin. For the production of HSE, we used available primary PCS-200-010, PCS-201-010, PCS-200-013, and immortalized CRL-4048 and CRL-4001 cell lines. We used genipin, which is necessary for collagen cross-linking and studied its cytotoxicity for keratinocytes and fibroblasts. The addition of 20 μM genipin reduced the shrinkage of the collagen in the constructs from 59% to 24% on day 12 of the culture of the construct. A higher concentration (80–200 µM) of genipin reduced shrinkage by 14% on average. Genipin in concentration 10 μM and below was not cytotoxic to the keratinocytes, and 150 μM and below to the fibroblasts. Hematoxylin and eosin staining showed that the morphology of HSEs was identical to that of native human skin. The immunohistochemical staining of the constructs showed the presence of vimentin-positive fibroblasts in the skin layer, while the melanocytes were in the epidermis and in the basal layer. We observed that the longer differentiation of constructs led to the higher secretion of GM-CSF, IL-10, IL-15, IL-1α, IL-6, IL-7, IL-8, and MCP-1. We also observed that the longer time of differentiation led to a more stable secretion of all analytes, which was reflected in the coefficient of variation. We described here a simple, reliable, and cost-effective production of the full-thickness human skin equivalents that can be used in the research and industry. With the global trend to decrease animal use for the research and testing, our HSE could be a useful testing tool and an alternative research model.
Small-Angle Scattering and Multifractal Analysis of DNA Sequences
Authors: Anitas Eugen Mircea
Published: June 2020

The arrangement of A, C, G and T nucleotides in large DNA sequences of many prokaryotic and eukaryotic cells exhibit long-range correlations with fractal properties. Chaos game representation (CGR) of such DNA sequences, followed by a multifractal analysis, is a useful way to analyze the corresponding scaling properties. This approach provides a powerful visualization method to characterize their spatial inhomogeneity, and allows discrimination between mono- and multifractal distributions. However, in some cases, two different arbitrary point distributions, may generate indistinguishable multifractal spectra. By using a new model based on multiplicative deterministic cascades, here it is shown that small-angle scattering (SAS) formalism can be used to address such issue, and to extract additional structural information. It is shown that the box-counting dimension given by multifractal spectra can be recovered from the scattering exponent of SAS intensity in the fractal region. This approach is illustrated for point distributions of CGR data corresponding to Escherichia coli, Phospholamban and Mouse mitochondrial DNA, and it is shown that for the latter two cases, SAS allows extraction of the fractal iteration number and the scaling factor corresponding to “ACGT” square, or to recover the number of bases. The results are compared with a model based on multiplicative deterministic cascades, and respectively with one which takes into account the existence of forbidden sequences in DNA. This allows a classification of the DNA sequences in terms of random and deterministic fractals structures emerging in CGR.
Combination of HIV-1 and Diabetes Enhances Blood Brain Barrier Injury via Effects on Brain Endothelium and Pericytes
Authors: Rom Slava , Gajghate Sachin , Winfield Malika , Reichenbach Nancy L. , Persidsky Yuri
Published: June 2020

Despite combined antiretroviral therapy (ART) achieving efficient HIV replication control, HIV-associated neurocognitive disorders (HAND) continue to be highly prevalent in HIV-infected patients. Diabetes mellitus (DM) is a well-known comorbidity of HAND in HIV-infected patients. Blood brain barrier (BBB) dysfunction has been linked recently to dementia development, specifically in DM patients. BBB injury exists both in HIV and DM, likely contributing to cognitive decline. However, its extent, exact cellular targets and mechanisms are largely unknown. In this report, we found a decrease in pericyte coverage and expression of tight junction proteins in human brain tissues from HIV patients with DM and evidence of HAND when compared to HIV-infected patients without DM or seronegative DM patients. Using our in vitro BBB models, we demonstrated diminution of barrier integrity, enhanced monocyte adhesion, changes in cytoskeleton and overexpression of adhesion molecules in primary human brain endothelial cells or human brain pericytes after exposure to HIV and DM-relevant stimuli. Our study demonstrates for the first-time evidence of impaired BBB function in HIV-DM patients and shows potential mechanisms leading to it in brain endothelium and pericytes that may result in poorer cognitive performance compared to individuals without HIV and DM.
Signaling Through Purinergic Receptor P2Y2 Enhances Macrophage IL-1β Production
Authors: de la Rosa Gonzalo , Gómez Ana I. , Baños María C. , Pelegrín Pablo
Published: June 2020

The release of nucleotides during necrosis or apoptosis has been described to have both proinflammatory and anti-inflammatory effect on the surrounding cells. Here we describe how low concentrations of UTP and ATP applied during macrophage priming enhance IL-1β production when subsequently the NLRP3 inflammasome is activated in murine resident peritoneal macrophages. Deficiency or pharmacological inhibition of the purinergic receptor P2Y2 reverted the increase of IL-1β release induced by nucleotides. IL-1β increase was found dependent on the expression of Il1b gene and probably involving JNK activity. On the contrary, nucleotides decreased the production of a different proinflammatory cytokines such as TNF-α. These results suggest that nucleotides could shape the response of macrophages to obtain a unique proinflammatory signature that might be relevant in unrevealing specific inflammatory conditions.
Immune Checkpoint Inhibitors in the Treatment of Renal Cancer: Current State and Future Perspective
Authors: Lavacchi Daniele , Pellegrini Elisa , Palmieri Valeria Emma , Doni Laura , Mela Marinella Micol , Di Maida Fabrizio , Amedei Amedeo , Pillozzi Serena , Carini Marco , Antonuzzo Lorenzo
Published: June 2020

Systemic treatment of renal cancer (RCC) has undergone remarkable changes over the past 20 years with the introduction of immunotherapeutic agents targeting programmed cell death (PD-1)/programmed death-ligand 1 (PD-L1) axis, as a single-agent or combined with anti-CTLA-4 monoclonal antibodies (MoAbs) or a multi-target vascular endothelial growth factor-(VEGF) tyrosine kinase inhibitor (TKI). In this paper, we review the main evidence on the use of Immune Checkpoint Inhibitors (ICIs) for RCC treatment from the first demonstration of activity of a nivolumab single agent in a phase I trial to the novel combination strategies (anti-PD-1 plus anti-CTLA4 or anti-PD-1 plus TKI). In addition, we discuss the use of anti-PD-1/PD-L1 agents in patients with non-clear cells and rare histological subtype RCC. Then, we critically examine the current findings in biomarkers that have been proposed to be prognostic or predictive to the response of immunotherapy including immune gene expression signature, B7-H1 expression, PBRM1 loss of function, PD-L1 expression, frame shift indel count, mutations in bromodomain-containing genes in patients with MiT family translocation RCC (tRCC), high expression of the T-effector gene signature, and a high myeloid inflammation gene expression pattern. To date, a single biomarker as a predictor of response has not been established. Since the dynamic behavior of the immune response and the different impact of ICI treatment on patients with specific RCC subtypes, the integration of multiple biomarkers and further validation in clinical trials are needed.
Role of ROS/RNS in Preeclampsia: Are Connexins the Missing Piece?
Authors: Rozas-Villanueva María F. , Casanello Paola , Retamal Mauricio A.
Published: June 2020

Preeclampsia is a pregnancy complication that appears after 20 weeks of gestation and is characterized by hypertension and proteinuria, affecting both mother and offspring. The cellular and molecular mechanisms that cause the development of preeclampsia are poorly understood. An important feature of preeclampsia is an increase in oxygen and nitrogen derived free radicals (reactive oxygen species/reactive nitrogen species (ROS/RNS), which seem to be central players setting the development and progression of preeclampsia. Cell-to-cell communication may be disrupted as well. Connexins (Cxs), a family of transmembrane proteins that form hemichannels and gap junction channels (GJCs), are essential in paracrine and autocrine cell communication, allowing the movement of signaling molecules between cells as well as between the cytoplasm and the extracellular media. GJCs and hemichannels are fundamental for communication between endothelial and smooth muscle cells and, therefore, in the control of vascular contraction and relaxation. In systemic vasculature, the activity of GJCs and hemichannels is modulated by ROS and RNS. Cxs participate in the development of the placenta and are expressed in placental vasculature. However, it is unknown whether Cxs are modulated by ROS/RNS in the placenta, or whether this potential modulation contributes to the pathogenesis of preeclampsia. Our review addresses the possible role of Cxs in preeclampsia, and the plausible modulation of Cxs-formed channels by ROS and RNS. We suggest these factors may contribute to the development of preeclampsia.
Background Glucocorticoid Therapy Has No Impact on Efficacy and Safety of Abatacept or Adalimumab in Patients with Rheumatoid Arthritis
Authors: Degboé Yannick , Schiff Michael , Weinblatt Michael , Fleischmann Roy , Ahmad Harris A. , Constantin Arnaud
Published: June 2020

To date, the impact of background glucocorticoids (GC) on the efficacy and safety of abatacept or adalimumab in patients with active rheumatoid arthritis (RA) is not clearly established. This post hoc analysis of (AMPLE) trial (NCT00929864) compared efficacy and safety outcomes over 2 years in patients treated with abatacept or adalimumab plus background methotrexate (MTX), who continued GC (≤10 mg/day) versus those who were not receiving GC (no-GC). Of 646 randomized patients, 317 received abatacept + MTX (161 GC, 156 no-GC) and 326 received adalimumab + MTX (162 GC, 164 no-GC). At Year 2, the adjusted mean changes from baseline in Disease Activity Score (DAS28 C-reactive protein (CRP)) and Health Assessment Questionnaire-Disability Index (HAQ-DI) were not significantly different in the GC versus no-GC subgroups receiving abatacept or adalimumab. A similar proportion of patients achieved remission, HAQ-DI score improvement ≥0.3 and radiographic progression rates. No clinically meaningful safety differences were observed between GC versus no-GC subgroups either with abatacept or adalimumab. In patients with active RA of similar baseline disease activity treated with abatacept or adalimumab plus background MTX, there was no additional value of background GC on clinical, functional or radiographic outcomes over two years.