bluuebunny/crossref_metadata_2025 huggingface dataset:
Classification Criteria for ANCA Associated Vasculitis – Ready for Prime Time?
Authors: Rathmann Jens , Mohammad Aladdin J.
Published: June 2024

Purpose of Review

This review aims to summarize the evolution and recent developments in the classification of ANCA associated vasculitis (AAV) and to summarize evaluations of the 2022 ACR/EULAR classification criteria of AAV in several cohorts.

Recent Findings

The classification of AAV has been a field of controversy for some time. The parallel existence of classification criteria and disease definitions produced some overlap in classification, leading to challenges when comparing different cohorts. The 2022 ACR/EULAR classification criteria derived from the largest study ever conducted in vasculitis account for significant changes in vasculitis classification with the integration of ANCA and modern imaging. These criteria show good performance compared to previous ones but also raise questions as ANCA serotypes have substantial impact on classification. In addition, there are some discrepancies with earlier agreed histopathological features of AAV disease phenotypes.

During the last 35 years, several sets of classification criteria have evolved to facilitate epidemiologic studies and clinical trials in AAV. While some of these criteria have been in use for many years, they were criticized due to either not using ANCA or not integrating surrogate markers for vasculitis but also due to overlapping when used in parallel. The long-awaited new ACR/EULAR criteria for AAV were published in 2022 and are the result of a large international study, introducing for the first time ANCA and modern imaging in the classification of AAV. Though the criteria show good performance, they bring several other challenges with practical application.
Interaction Between Aging-Related Elastin-Derived Peptide (VGVAPG) and Sirtuin 2 and its Impact on Functions of Human Neuron Cells in an In Vitro Model
Authors: Skóra Bartosz , Piechowiak Tomasz , Szychowski Konrad A.
Published: June 2024

Elastin is a stable protein present in many tissues, including brain tissues, and is one of the most long-life proteins with a half-life of approximately 70 years. The peptide with a Val-Gly-Val-Ala-Pro-Gly (VGVAPG) amino acid sequence is released during elastin decay, which correlates with aging-related neurodegeneration. A recent study has shown enhanced protein expression of Sirtuin 2 (SIRT2 – one of the redox homeostatic factors) in aged rodent brains, while the correlation between VGVAPG and SIRT2 has never been evaluated so far. Therefore, the study aimed to determine the impact of the VGVAPG hexapeptide on SIRT2 and neuronal functions in differentiated SH-SY5Y cells at the gene and protein expression levels. The present results showed that VGVAPG caused a 52.69% decrease in the level of reactive oxygen species (ROS), as in the case of neurons treated with AGK2 (Sirtuin 2 inhibitor) after 24h and 48h. Furthermore, a decrease in superoxide dismutase (SOD) activity was observed. The SIRT2 gene expression was found to fluctuate after 6h and 24h as a result of the exposure to the VGVAPG peptide. In turn, a decrease in the PPARγ, P53, SOD2, and CAT mRNA expression was shown in VGVAPG-treated cells. Additionally, an increase in the Sirtuin 2 protein expression was recorded after 24h and 48h in the VGVAPG peptide-treated neurons. Last but not least, the decrease in the level of acetylation of α-tubulin after the hexapeptide treatment was correlated with shortening of neurites, which may indicate the destabilization of the microtubule and ROS-independent induction of neurodegeneration.

Graphical Abstract
Trauma in Publishing
Authors: Otmar Renée
Published: June 2024

We live in an extraordinary time in human history. Arguably, the most significant social changes have taken place in recent decades, including changes in policy and attitudes towards human rights, how we educate and treat children, towards sexual violence and abuse, sexuality and gender identification, to name just a few. In Australia, we have witnessed public responses through Royal Commissions and other public inquiries that have given voice to generations of people whose suffering had been silenced by formal and informal social mechanisms. Now, in the twenty-first century, the proliferation of new technologies, social media and social campaigns designed to engage people online as well as offline have empowered citizens to take control of their narratives, to tell their own stories. In the published literature, a great many have begun to reveal the nature and extent of traumas experienced by individuals, families, groups and entire communities. In the publishing industry, scant attention is given to the occupational risks faced by the workforce charged with disseminating these trauma stories: the editors, literary agents, proofreaders, publicists and other publishing personnel who are repeatedly exposed to trauma narratives. Even as publishing facilitates healing through story, it is also perpetuating—or at the very least enabling—the transmission of trauma: from author to publishing personnel, and quite possibly also from industry to reader. This paper posits that publishing as an industry is awash with trauma. Through the perspective of an industry insider, it explores the three faces of trauma in the publishing context: recounting trauma as a vehicle for healing and reconciliation, the trauma narrative as a commodity and vicarious trauma as an occupational hazard.
A brief report on nanophotonics and metamaterials landscape in India
Authors: Joshi Krishna , Sahoo Himadri , Mujumdar Sushil
Published: June 2024

Here, we describe a set of research results in the domain of Nanophotonics and Metamaterials that represent the broad N &M landscape in India. These results were presented in an online BRICS meeting, and were collated based on the criteria deemed appropriate for the said forum. Results presented at the meeting encompass various areas, including nano-optics, nano-opto-mechanics, integrated photonic devices, plasmonics, metal-enhanced fluorescence, bio relevant photonics and metamaterials. Research topics such as Anderson localization of light, exceptional points in non-Hermitian systems, manipulation of nanoscale mechanical motion, efficient mode coupling in integrated photonics etc are discussed. Furthermore, miniaturized SPR sensors, coupling between metal nanostructures and semiconductor quantum dots, biosensing applications, metamaterials and random lasing, and customizable optical functionalities for sensing, and energy conversion are also elaborated upon. In the end, a brief listing of more recent selected publications is presented. This review article highlights the diverse and promising avenues in nanophotonics and metamaterials research in India.
The Manager and Love: Evoking a Loving Inquiry in a Group Setting
Authors: Chen Angela P. , Nigri Giorgia , Culham Thomas E. , Nussbaum Barbara , Peregoy Richard , Plunkett Margot
Published: June 2024

Neuroscientists, psychologists, educators, and management scholars propose that the current emphasis on intellect and reason in education and business over values such as love, connectedness, and compassion are at the root of many business ethical failures and societal problems. They argue not that reason should be abandoned in education and business management but rather that it needs to be balanced with values such as love because these attributes are innately human, enabling wise decision-making. This is a difficult task in the context of the current ethos of intellect and reason that dominates education and management. To correct the imbalance, we must explore ways of preparing future managers to accept the relevance and importance of learning to develop and embody love. Through our research, we provide an experience of community love by creating a caring, receptive, personal container. We engaged in the practice of Collaborative Autoethnography, integrating the Nguni South African concept of Ubuntu, to explore, research, and demonstrate the experience of love in a community setting. To support this practice, we framed it against the background of integrative justice, focusing on authentic engagement without exploitative intent as per Santos and Laczniak’s (2015) Integrative Justice Model (IJM) and built upon some common contexts from which love is considered such as Catholic Social Thought (CST) and indigenous cultures. We analyzed why and how love might be implemented in education and management and how Collaborative Autoethnography can be applied in connecting with others to research, learn from, and build upon the experience of love and connectedness.
Development of lightweight intrusion model in Industrial Internet of Things using deep learning technique
Authors: Sinha Raj , Thakur Padmanabh , Gupta Sandeep , Shukla Anand
Published: June 2024

Nowadays, the IDS is being used in conjunction with the IIoT system to reduce the security risk, but on the other hand, the false rate of the IDS is very high. Therefore, in this work, a pre-training method, making use of both a deep neural network and a deep auto-encoder, has been proposed for the quick prediction of assaults with increased accuracy and a reduced false rate. The replicas were expanded using hyperparameter optimization (HPO) techniques. The proposed model delivers an alternative to deep learning construction replicas through an HPO procedure incorporating the Archimedes optimization algorithm. This optimization technique can be used to determine the hyperparameter value and the ideal categorical hyperparameter combination for improved detection performance. The DS2OS dataset is used alongside numerous other indicators to evaluate the efficacy of the developed model. The various existing techniques of assault detection have also been considered to show the effectiveness of the proposed model. Through the comparative evaluation of the outcomes, it is shown that the developed model provides better performance than the other existing models. Eventually, it is discovered that the suggested security paradigm is successful in fending off a variety of internal and external threats.
White Clover does not Increase Soil N2O Emissions Compared to Ryegrass in Non-Frozen Winter, but Increases CH4 Uptake
Authors: Shao Guodong , Ai Juanjuan , Dippold Michaela A. , Fan Lichao , Dorodnikov Maxim , Liang Bo , Dittert Klaus , Wang Haitao
Published: June 2024

As one of the most important forage species in Europe, white clover (Trifolium repens) is a legume that is well recognized for its potential to increase productivity especially under reduced N input. It is hypothesized that legumes have the potential to decrease overwinter soil greenhouse gas (GHG) emissions due to more efficient N recycling as compared to non-legume forbs. We conducted a field experiment recording high-resolution soil nitrous oxide (N2O) and methane (CH4) fluxes during the winter months (December 2019 to March 2020) on a five-year-old grassland in central Germany with white clover, fertilized and unfertilized perennial ryegrass (Lolium perenne), and bare soil. White clover and fertilized ryegrass stimulated soil N2O emissions by 174% and 212% as compared to bare soil, and by 36% and 56% as compared to unfertilized ryegrass, respectively, due to their greater N availability and higher water-filled pore space (WFPS). The estimated cumulative CH4 fluxes under white clover were a net CH4 sink, whereas ryegrass and bare soil were net CH4 sources. Soil N2O fluxes were predominantly regulated by both mineral N and WFPS, while CH4 fluxes were mainly explained by WFPS. N-fertilization during the growing season did not affect off-season N2O and CH4 fluxes in perennial ryegrass plots. The combined non-CO2 global warming potential highlighted the possible mitigation effect of white clover on overwinter GHG emissions. Our findings suggest that GHG emissions from legumes are not offsetting their productive benefits during the non-frozen winter seasons.
Exploring magnetic nanomaterials with a focus on magnetic biochar in anaerobic digestion: from synthesis to application
Authors: Zhou Wenneng , Mazarji Mahmoud , Li Mengtong , Li Aohua , Wang Yajing , Yang Yadong , Lee Jonathan T. E. , Rene Eldon R. , Yuan Xiangzhou , Pan Junting
Published: June 2024

Anaerobic digestion technology, effective for sustainable waste management and renewable energy, but challenged by slow reaction rates and low biogas yields, could benefit from advancements in magnetic nanomaterials. This review explores the potential of magnetic nanomaterials, particularly magnetic biochar nanocomposites, to address these challenges by serving as electron conduits and providing essential iron. This review contributes a thorough overview of the application of magnetic nanoparticles loaded into biochar in anaerobic digestion and engages in a comprehensive discussion regarding the synthesis methods and characterization of various magnetic nanoparticles, elucidating their mechanisms of action in both the absence and presence of magnetic fields. Our review underscores the predominance of co-precipitation (53%) and commercially sourced nanoparticles (29%) as the main synthesis methods, with chemical reduction, pyrolysis, and green synthesis pathways less commonly utilized (8%, 5%, and 5%, respectively). Notably, pyrolysis is predominantly employed for synthesizing magnetic biochar nanocomposites, reflecting its prevalence in 100% of cases for this specific application. By offering a critical evaluation of the current state of knowledge and discussing the challenges and future directions for research in this field, this review can help researchers and practitioners better understand the potential of magnetic biochar nanocomposites for enhancing anaerobic digestion performance and ultimately advancing sustainable waste management and renewable energy production.

Graphical Abstract
Welcher Sport für wen?
Authors: Schmid Julia , Gut Vanessa , Schorno Nina , Sudeck Gorden , Conzelmann Achim
Published: June 2024

Wie die optimale Passung zwischen Person und Sportaktivität in der Praxis gelingen kann

Obwohl die positiven Gesundheitswirkungen von körperlicher Aktivität allgemein bekannt sind, tun sich viele Menschen schwer, mit Sporttreiben zu beginnen und dabei zu bleiben. Möchte man das regelmäßige Sporttreiben fördern, ist zu beachten, dass sowohl wir Menschen als auch der Sport sehr vielfältig sind. Ziel ist demzufolge, eine möglichst gute Passung zwischen Person und sportlicher Aktivität herzustellen. Insbesondere die Beweggründe einer Person (z. B. Kontakt im Sport) und die Anreize einer Sportaktivität (z. B. geselliges Miteinander im Fußball) sollten aufeinander abgestimmt werden. Dies kann durch die Entwicklung von maßgeschneiderten Sportangeboten, die Anpassung bzw. Erweiterung einer bestehenden Sportangebotspalette oder die Durchführung einer individuellen Sportberatung passieren. Gelingt auf diesem Wege eine optimale Passung, so fühlen wir uns während des Sports wohl und bleiben eher dran.

In diesem Buch erhalten Expertinnen und Experten im Erwachsenensport und in der Gesundheitsförderung, Fachkräfte in der Rehabilitation, aber auch Studierende und Dozierende der Sportwissenschaft Antworten auf folgende Fragen:

Welche Beweggründe zum Sporttreiben existieren und wie können sie erfasst werden?
Wie können Menschen anhand ihrer Beweggründe gruppiert werden?
Wie kann ich Sporttypen bestimmen?
Wie kann eine optimale Passung zwischen den Beweggründen einer Person und den Anreizen einer Sportaktivität hergestellt werden?
Evolution of Energetic Proton Parallel Pressure Anisotropy at Geosynchronous Altitudes: Potential Role in Triggering Substorm Expansion Phase Onset
Authors: Babu S. S. , Mann I. R. , Dimitrakoudis S. , Ozeke L. G. , Rae I. J. , Forsyth C. , Smith A. W.
Published: June 2024

The sequence of events associated with the triggering of energy release during substorm expansion phase onset is still not well‐understood. Oberhagemann and Mann (2020b, https://doi.org/10.1029/2019gl085271) proposed a new substorm onset mechanism, where the transition toward parallel proton pressure anisotropy during tail stretching in the late growth phase could trigger a pressure anisotropic ballooning instability. Here we examine the evolution of energetic proton parallel pressure anisotropy at geosynchronous altitudes, seeking evidence in support of the proposed substorm onset mechanism. We use the Geostationary Operational Environment Satellite (GOES) proton flux and magnetometer data combined with substorm onset indicators derived from ground‐based magnetometers. Superposed epoch analysis of substorm onset times for 2014 using the isolated substorm list (Ohtani & Gjerloev, 2020, https://doi.org/10.1029/2020ja027902) clearly shows signatures of energetic proton parallel pressure anisotropy immediately before substorm onset, potentially supportive of the Oberhagemann and Mann theory.
Periodicity in the Deccan Volcanism Modulated by Plume Perturbations at the Mid‐Mantle Transition Zone
Authors: Ghosh Dip , Sen Joyjeet , Mandal Nibir
Published: June 2024

In peninsular India, the Deccan Traps record massive, continental‐scale volcanism in a sequence of magmatic events that corresponds with the timing of mass extinction at the Cretaceous‐Paleogene boundary. Although the Deccan volcanism is linked with the Réunion hotspot, the origin of its periodic magmatic pulses is still debated. We developed a numerical model replicating the geodynamic scenario of the African superplume underneath a moving Indian plate to explore the mechanism of magmatic pulse generation during the Deccan volcanism. Our model results revealed a connection between the Réunion hotspot and the African large low shear‐wave velocity province (LLSVP), suggesting that the pulses were produced from a thermochemical plume originated in the lower mantle. The ascending plume had stagnation at 660 km due to phase changes in the transition zone, and its head eventually underwent detachment from the tail under the influence of Indian plate movement to produce sequentially four major pulses (periodicity: 5–8 Ma), each giving rise to multiple secondary magmatic pulses at a time interval of ∼0.15–0.4 Ma. This study sheds a new light on the mechanism of periodic hotspot activities from a global perspective.
Resolving Organic Aerosol Components Contributing to the Oxidative Potential of PM2.5 in the North China Plain
Authors: Liu Fobang , Yang Xu , Xu Weiqi , Verma Vishal , Wang Zhao , Chen Chun , He Yao , Yang Liu , Yang Yang , Sun Yele , He Chi
Published: June 2024

The oxidative potential (OP) of ambient particulate matter (PM) is a common metric for estimating PM toxicity and linking PM exposure to adverse health effects. Organic aerosol (OA), a dominant fraction of ambient PM worldwide, may significantly contribute to PM toxicity. Here, we investigated the source‐based OA components contributing to the OP of PM in the urban (Beijing, summer and winter) and rural (Gucheng, winter) environments of the North China Plain (NCP). Various OA components as identified by the aerosol mass spectrometer/aerosol chemical speciation monitor (AMS/ACSM), transition metals, and black carbon were compared with the OP of PM measured by dithiothreitol assays. The results consistently demonstrate the importance of OA as a contributor to PM's OP in both urban and rural NCP environments. Higher intrinsic OP was observed in winter Beijing than in summer, possibly due to OA being predominantly from anthropogenic sources in winter. Furthermore, different OA components were found to drive the response of OP in the two environments. More‐oxidized oxygenated OA (MO‐OOA), cooking OA, and oxidized primary OA (during winter) are the OA contributors to OP in the urban environment, with a dominant contribution from MO‐OOA. In contrast, biomass burning OA (BBOA) and OOA play a major role in the OP in the rural environment, with BBOA making the largest contribution. Overall, this work highlights the significance of OA in determining PM's OP and calls for more work to reveal the sources and characteristics of OA components contributing to OP across different regions.
Amino acid influx via LAT1 regulates iron demand and sensitivity to PPMX-T003 of aggressive natural killer cell leukemia
Authors: Yanagiya Ryo , Miyatake Yuji , Watanabe Natsumi , Shimizu Takanobu , Kanamori Akane , Ueno Masaya , Okabe Sachiko , Carreras Joaquim , Nakayama Shunya , Hasegawa Ami , Kameda Kazuaki , Kamakura Takeshi , Nakagawa So , Yamauchi Takuji , Maeda Takahiro , Ishii Keisuke , Matsuura Tadashi , Handa Hiroshi , Hirao Atsushi , Ishizawa Kenichi , Onizuka Makoto , Mashima Tetsuo , Nakamura Naoya , Ando Kiyoshi , Kotani Ai
Published: June 2024

Aggressive natural killer cell leukemia (ANKL) is a rare hematological malignancy with a fulminant clinical course. Our previous study revealed that ANKL cells proliferate predominantly in the liver sinusoids and strongly depend on transferrin supplementation. In addition, we demonstrated that liver-resident ANKL cells are sensitive to PPMX-T003, an anti-human transferrin receptor 1 inhibitory antibody, whereas spleen-resident ANKL cells are resistant to transferrin receptor 1 inhibition. However, the microenvironmental factors that regulate the iron dependency of ANKL cells remain unclear. In this study, we first revealed that the anti-neoplastic effect of PPMX-T003 was characterized by DNA double-strand breaks in a DNA replication-dependent manner, similar to conventional cytotoxic agents. We also found that the influx of extracellular amino acids via LAT1 stimulated sensitivity to PPMX-T003. Taken together, we discovered that the amount of extracellular amino acid influx through LAT1 was the key environmental factor determining the iron dependency of ANKL cells via adjustment of their mTOR/Myc activity, which provides a good explanation for the different sensitivity to PPMX-T003 between liver- and spleen-resident ANKL cells, as the liver sinusoid contains abundant amino acids absorbed from the gut.
Curcumol ameliorates neuroinflammation after cerebral ischemia–reperfusion injury via affecting microglial polarization and Treg/Th17 balance through Nrf2/HO-1 and NF-κB signaling
Authors: Liu Ying , Wang Wen , Di Bohan , Miao Jiangyong
Published: June 2024

Neuroinflammation caused by microglia and other immune cells plays pivotal role in cerebral ischemia/reperfusion injury and recovery. Modulating microglial polarization or Treg/Th17 balance from pro-inflammatory phenotype to anti-inflammatory phenotype are promising strategies for the treatment of cerebral ischemia. Curcumol has potential to fight against oxidative stress and inflammation, but whether it has protective effect in cerebral ischemia is uncertain. In the present study, cerebral ischemia was induced in C57BL/6 mice via middle cerebral artery occlusion (MCAO). MCAO mice were treated with curcumol for 7 days, then post-stroke ischemic injury, neurological deficits, microglial polarization and brain leukocyte infiltration were evaluated by TTC staining, behavioural tests, flow cytometry, western blot and immunofluorescence. We found that poststroke administration of curcumol reduced infarct volume, attenuated neuronal damage and inflammation, and improved motor function recovery of MCAO mice. Curcumol skewed microglial polarization toward anti-inflammatory phenotype in MCAO mice in vivo or after oxygen-glucose deprivation and reoxygenation (OGD/R) in vitro. In addition, curcumol reduced local T cell infiltration in ischemic brain of MCAO mice and impaired Treg/Th17 balance. Curcumol inhibited ROS production and regulated Nrf2/HO-1 and NF-κB signaling in microglia. Finally, inhibiting Nrf2/HO-1 signaling or activating NF-κB signaling abrogated the influence of curcumol on microglial polarization. In conclusion, curcumol treatment reduced brain damage and neuroinflammation via modulating anti-inflammatory microglial polarization and Treg/Th17 balance through Nrf2/HO-1 and NF-κB signaling. Curcumol might be a promising treatment strategy for stroke patients.
Prefrontal coding of learned and inferred knowledge during REM and NREM sleep
Authors: Abdou Kareem , Nomoto Masanori , Aly Mohamed H. , Ibrahim Ahmed Z. , Choko Kiriko , Okubo-Suzuki Reiko , Muramatsu Shin-ichi , Inokuchi Kaoru
Published: June 2024

Idling brain activity has been proposed to facilitate inference, insight, and innovative problem-solving. However, it remains unclear how and when the idling brain can create novel ideas. Here, we show that cortical offline activity is both necessary and sufficient for building unlearned inferential knowledge from previously acquired information. In a transitive inference paradigm, male C57BL/6J mice gained the inference 1 day after, but not shortly after, complete training. Inhibiting the neuronal computations in the anterior cingulate cortex (ACC) during post-learning either non-rapid eye movement (NREM) or rapid eye movement (REM) sleep, but not wakefulness, disrupted the inference without affecting the learned knowledge. In vivo Ca2+ imaging suggests that NREM sleep organizes the scattered learned knowledge in a complete hierarchy, while REM sleep computes the inferential information from the organized hierarchy. Furthermore, after insufficient learning, artificial activation of medial entorhinal cortex-ACC dialog during only REM sleep created inferential knowledge. Collectively, our study provides a mechanistic insight on NREM and REM coordination in weaving inferential knowledge, thus highlighting the power of idling brain in cognitive flexibility.
Improving the representativeness of UK’s national COVID-19 Infection Survey through spatio-temporal regression and post-stratification
Authors: Pouwels Koen B. , Eyre David W. , House Thomas , Aspey Ben , Matthews Philippa C. , Stoesser Nicole , Newton John N. , Diamond Ian , Studley Ruth , Taylor Nick G. H. , Bell John I. , Farrar Jeremy , Kolenchery Jaison , Marsden Brian D. , Hoosdally Sarah , Jones E. Yvonne , Stuart David I. , Crook Derrick W. , Peto Tim E. A. , Walker A. Sarah ,   the COVID−19 Infection Survey Team, Wei Jia , Pritchard Emma , Vihta Karina-Doris , Doherty George , Kavanagh James , Chau Kevin K. , Hatch Stephanie B. , Ebner Daniel , Ferreira Lucas Martins , Christott Thomas , Dejnirattisai Wanwisa , Mongkolsapaya Juthathip , Cameron Sarah , Tamblin-Hopper Phoebe , Wolna Magda , Brown Rachael , Cornall Richard , Screaton Gavin , Lythgoe Katrina , Bonsall David , Golubchik Tanya , Fryer Helen , Thomas Tina , Ayoubkhani Daniel , Black Russell , Felton Antonio , Crees Megan , Jones Joel , Lloyd Lina , Sutherland Esther , Cox Stuart , Paddon Kevin , James Tim , Robotham Julie V. , Birrell Paul , Jordan Helena , Sheppard Tim , Athey Graham , Moody Dan , Curry Leigh , Brereton Pamela , Jarvis Ian , Godsmark Anna , Morris George , Mallick Bobby , Eeles Phil , Hay Jodie , VanSteenhouse Harper , Lee Jessica , White Sean , Evans Tim , Bloemberg Lisa , Allison Katie , Pandya Anouska , Davis Sophie , Conway David I. , MacLeod Margaret , Cunningham Chris
Published: June 2024

Population-representative estimates of SARS-CoV-2 infection prevalence and antibody levels in specific geographic areas at different time points are needed to optimise policy responses. However, even population-wide surveys are potentially impacted by biases arising from differences in participation rates across key groups. Here, we used spatio-temporal regression and post-stratification models to UK’s national COVID-19 Infection Survey (CIS) to obtain representative estimates of PCR positivity (6,496,052 tests) and antibody prevalence (1,941,333 tests) for different regions, ages and ethnicities (7-December-2020 to 4-May-2022). Not accounting for vaccination status through post-stratification led to small underestimation of PCR positivity, but more substantial overestimations of antibody levels in the population (up to 21 percentage points), particularly in groups with low vaccine uptake in the general population. There was marked variation in the relative contribution of different areas and age-groups to each wave. Future analyses of infectious disease surveys should take into account major drivers of outcomes of interest that may also influence participation, with vaccination being an important factor to consider.
Creation of a biological sensorimotor interface for bionic reconstruction
Authors: Festin Christopher , Ortmayr Joachim , Maierhofer Udo , Tereshenko Vlad , Blumer Roland , Schmoll Martin , Carrero-Rojas Génova , Luft Matthias , Laengle Gregor , Farina Dario , Bergmeister Konstantin D. , Aszmann Oskar C.
Published: June 2024

Neuromuscular control of bionic arms has constantly improved over the past years, however, restoration of sensation remains elusive. Previous approaches to reestablish sensory feedback include tactile, electrical, and peripheral nerve stimulation, however, they cannot recreate natural, intuitive sensations. Here, we establish an experimental biological sensorimotor interface and demonstrate its potential use in neuroprosthetics. We transfer a mixed nerve to a skeletal muscle combined with glabrous dermal skin transplantation, thus forming a bi-directional communication unit in a rat model. Morphological analyses indicate reinnervation of the skin, mechanoreceptors, NMJs, and muscle spindles. Furthermore, sequential retrograde labeling reveals specific sensory reinnervation at the level of the dorsal root ganglia. Electrophysiological recordings show reproducible afferent signals upon tactile stimulation and tendon manipulation. The results demonstrate the possibility of surgically creating an interface for both decoding efferent motor control, as well as encoding afferent tactile and proprioceptive feedback, and may indicate the way forward regarding clinical translation of biological communication pathways for neuroprosthetic applications.
Safeguarding China’s long-term sustainability against systemic disruptors
Authors: Li Ke , Gao Lei , Guo Zhaoxia , Dong Yucheng , Moallemi Enayat A. , Kou Gang , Chen Meiqian , Lin Wenhao , Liu Qi , Obersteiner Michael , Pedercini Matteo , Bryan Brett A.
Published: June 2024

China’s long-term sustainability faces socioeconomic and environmental uncertainties. We identify five key systemic risk drivers, called disruptors, which could push China into a polycrisis: pandemic disease, ageing and shrinking population, deglobalization, climate change, and biodiversity loss. Using an integrated simulation model, we quantify the effects of these disruptors on the country’s long-term sustainability framed by 17 Sustainable Development Goals (SDGs). Here we show that ageing and shrinking population, and climate change would be the two most influential disruptors on China’s long-term sustainability. The compound effects of all disruptors could result in up to 2.1 and 7.0 points decline in the China’s SDG score by 2030 and 2050, compared to the baseline with no disruptors and no additional sustainability policies. However, an integrated policy portfolio involving investment in education, healthcare, energy transition, water-use efficiency, ecological conservation and restoration could promote resilience against the compound effects and significantly improve China’s long-term sustainability.
Fluorescence resonance energy transfer in atomically precise metal nanoclusters by cocrystallization-induced spatial confinement
Authors: Li Hao , Wang Tian , Han Jiaojiao , Xu Ying , Kang Xi , Li Xiaosong , Zhu Manzhou
Published: June 2024

Understanding the fluorescence resonance energy transfer (FRET) of metal nanoparticles at the atomic level has long been a challenge due to the lack of accurate systems with definite distance and orientation of molecules. Here we present the realization of achieving FRET between two atomically precise copper nanoclusters through cocrystallization-induced spatial confinement. In this study, we demonstrate the establishment of FRET in a cocrystallized Cu8(p-MBT)8(PPh3)4@Cu10(p-MBT)10(PPh3)4 system by exploiting the overlapping spectra between the excitation of the Cu10(p-MBT)10(PPh3)4 cluster and the emission of the Cu8(p-MBT)8(PPh3)4 cluster, combined with accurate control over the confined space between the two nanoclusters. Density functional theory is employed to provide deeper insights into the role of the distance and dipole orientations of molecules to illustrate the FRET procedure between two cluster molecules at the electronic structure level.
Evaluation of body shape as a human body composition assessment in isolated conditions and remote environments
Authors: Wong Michael C. , Bennett Jonathan P. , Leong Lambert T. , Liu Yong E. , Kelly Nisa N. , Cherry John , Kloza Kate , Li Bosco , Iuliano Sandra , Sibonga Jean , Sawyer Aenor , Ayton Jeff , Shepherd John A.
Published: June 2024

Individuals in isolated and extreme environments can experience debilitating side-effects including significant decreases in fat-free mass (FFM) from disuse and inadequate nutrition. The objective of this study was to determine the strengths and weaknesses of three-dimensional optical (3DO) imaging for monitoring body composition in either simulated or actual remote environments. Thirty healthy adults (ASTRO, male = 15) and twenty-two Antarctic Expeditioners (ABCS, male = 18) were assessed for body composition. ASTRO participants completed duplicate 3DO scans while standing and inverted by gravity boots plus a single dual-energy X-ray absorptiometry (DXA) scan. The inverted scans were an analog for fluid redistribution from gravity changes. An existing body composition model was used to estimate fat mass (FM) and FFM from 3DO meshes. 3DO body composition estimates were compared to DXA with linear regression and reported with the coefficient of determination (R2) and root mean square error (RMSE). ABCS participants received only duplicate 3DO scans on a monthly basis. Standing ASTRO meshes achieved an R2 of 0.76 and 0.97 with an RMSE of 2.62 and 2.04 kg for FM and FFM, while inverted meshes achieved an R2 of 0.52 and 0.93 with an RMSE of 2.84 and 3.23 kg for FM and FFM, respectively, compared to DXA. For the ABCS arm, mean weight, FM, and FFM changes were −0.47, 0.06, and −0.54 kg, respectively. Simulated fluid redistribution decreased the accuracy of estimated body composition values from 3DO scans. However, FFM stayed robust. 3DO imaging showed good absolute accuracy for body composition assessment in isolated and remote environments.
Preparation of polysulfone-based nanofiber Janus membrane for membrane distillation containing organic pollutants
Authors: Wu Zhuobin , Zheng Ke , Zhang Guichang , Huang Longwei , Zhou Shaoqi
Published: June 2024

Membrane distillation is an emerging wastewater treatment technology that harnesses low-grade heat as an energy source and exhibits potential for complete desalination. Nonetheless, two notable challenges hinder the practical application of this technology: membrane wetting and fouling. To counter these challenges, an innovative anti-fouling Janus membrane with asymmetric wettability was developed through electrospinning. The hydrophobic layer was formed using tetraethyl orthosilicate/polysulfone (PSF), and the superhydrophilic layer was created using polyvinylpyrrolidone (PVP)/PSF. A sensitive adhesion probe was used to assess the anti-fouling performance of the Janus membrane against oil. Molecular dynamics simulation suggested that PVP reduced the adsorption tendency of the membrane for humic acid (HA). Under experimental conditions involving saline water with HA and a saline oil–water emulsion, the non-Janus membrane suffered severe fouling, resulting in rapid water permeate flux decline. However, the Janus membrane demonstrated consistent permeate flux (26.84 LMH and 24.92 LMH) and an impressive salt rejection rate (> 99.99%). This study suggests that the Janus membrane, with its high permeate fluxes and remarkable resistance to fouling and wetting, could be an effective solution for wastewater treatment, with considerable potential for future application.
Water- and heat-activated dynamic passivation for perovskite photovoltaics
Authors: Wang Wei-Ting , Holzhey Philippe , Zhou Ning , Zhang Qiang , Zhou Suer , Duijnstee Elisabeth A. , Rietwyk Kevin J. , Lin Jeng-Yu , Mu Yijie , Zhang Yanfeng , Bach Udo , Wu Chun-Guey , Yip Hin‐Lap , Snaith Henry J. , Feng Shien-Ping
Published: June 2024

Further improvements in perovskite solar cells require better control of ionic defects in the perovskite photoactive layer during the manufacturing stage and their usage1–5. Here we report a living passivation strategy using a hindered urea/thiocarbamate bond6–8 Lewis acid–base material (HUBLA), where dynamic covalent bonds with water and heat-activated characteristics can dynamically heal the perovskite to ensure device performance and stability. Upon exposure to moisture or heat, HUBLA generates new agents and further passivates defects in the perovskite. This passivation strategy achieved high-performance devices with a power conversion efficiency (PCE) of 25.1 per cent. HUBLA devices retained 94 per cent of their initial PCE for approximately 1,500 hours of ageing at 85 degrees Celsius in nitrogen and maintained 88 per cent of their initial PCE after 1,000 hours of ageing at 85 degrees Celsius and 30 per cent relative humidity in air.
Single-cell epigenomic reconstruction of developmental trajectories from pluripotency in human neural organoid systems
Authors: Zenk Fides , Fleck Jonas Simon , Jansen Sophie Martina Johanna , Kashanian Bijan , Eisinger Benedikt , Santel Małgorzata , Dupré Jean-Samuel , Camp J. Gray , Treutlein Barbara
Published: June 2024

Cell fate progression of pluripotent progenitors is strictly regulated, resulting in high human cell diversity. Epigenetic modifications also orchestrate cell fate restriction. Unveiling the epigenetic mechanisms underlying human cell diversity has been difficult. In this study, we use human brain and retina organoid models and present single-cell profiling of H3K27ac, H3K27me3 and H3K4me3 histone modifications from progenitor to differentiated neural fates to reconstruct the epigenomic trajectories regulating cell identity acquisition. We capture transitions from pluripotency through neuroepithelium to retinal and brain region and cell type specification. Switching of repressive and activating epigenetic modifications can precede and predict cell fate decisions at each stage, providing a temporal census of gene regulatory elements and transcription factors. Removing H3K27me3 at the neuroectoderm stage disrupts fate restriction, resulting in aberrant cell identity acquisition. Our single-cell epigenome-wide map of human neural organoid development serves as a blueprint to explore human cell fate determination.
Method for lysis and paper-based elution-free DNA extraction with colourimetric isothermal amplification
Authors: Lee Soo Min , Doeven Egan H. , Yuan Dan , Guijt Rosanne M.
Published: June 2024

Nucleic acid amplification testing has great potential for point-of-need diagnostic testing with high detection sensitivity and specificity. Current sample preparation is limited by a tedious workflow requiring multiple steps, reagents and instrumentation, hampering nucleic acid testing at point of need. In this study, we present the use of mixed cellulose ester (MCE) paper for DNA binding by ionic interaction under molecular crowding conditions and fluid transport by wicking. The poly(ethylene) glycol-based (PEG) reagent simultaneously provides the high pH for alkaline lysis and crowding effects for ionic binding of the DNA under high salt conditions. In this study, we introduce Paper-based Abridged Solid-Phase Extraction with Alkaline Poly(ethylene) Glycol Lysis (PASAP). The anionic mixed cellulose ester (MCE) paper is used as solid phase and allows for fluid transport by wicking, eliminating the need for pipetting skills and the use of a magnet to retain beads. Following the release of DNA from the cells due to the lytic activity of the PASAP solution, the DNA binds to the anionic surface of the MCE paper, concentrating at the bottom while the sample matrix is transported towards the top by wicking. The paper was washed by dipping it in 40% isopropanol for 10 s. After air-drying for 30 s, the bottom section of the paper (3 mm × 4 mm) was snapped off using the cap of a PCR tube and immersed in the colourimetric loop-mediated isothermal amplification (cLAMP) solution for direct amplification and colourimetric detection. The total sample processing was completed in 15 min and ready for amplification. cLAMP enabled the detection of 102 CFU/mL of Escherichia coli (E. coli) from culture media and the detection of E. coli in milk < 103 CFU/mL (10 CFU) after incubation at 68 °C for 60 min, demonstrating applicability of the method to complex biological samples.
Association of physical activity and socio-economic status on mortality in older adults: a retrospective cohort study of KNHANES-mortality linked data
Authors: Lee Soomin , Ma Xiaonan , Choi Younghwan , Kim Yeon Soo
Published: June 2024

We examined the joint association of physical activity (PA) and socio-economic status (SES) on all-causes and cardiovascular disease (CVD) mortality in 6945 elderly Koreans (mean age: 71.6 years, 41.8% male) using data from the Korean National Health and Nutrition Examination Survey (2007–2013) and death data from Statistics Korea (2019). The SES included household income and education level. PA was assessed using the IPAQ and categorized according to the 2018 PA Guidelines. In stratified analyses using Cox proportional hazards by SES adherence to PA guidelines those who low household income group was associated with a reduced risk of all-cause mortality and CVD mortality, while in the lowest educational level group, it was associated with a reduced risk of all-cause mortality and CVD mortality. In the joint analysis, PA was associated with a significant reduction in all-cause mortality in all groups when compared with those who did not meet PA those who had the lowest SES. However, PA with CVD mortality risk was not significantly associated in the ‘upper-middle’ income and ‘high school’ education groups. The study revealed that PA significantly association mortality, particularly among older adults with low SES. This finding suggests the potential for targeted government interventions to promote healthy aging.
Prevalence of Helicobacter pylori resistance to certain antibiotics at An-Najah University Hospital: a cross-sectional study
Authors: Abdoh Qusay , Alnees Mohammad , Kharraz Lubna , Ayoub Khubaib , Darwish Abdalaziz , Awwad Mahdi , Najajra Duha , Khraim Jana , Awad Wafaa , Sbaih Aesha , Turman Safaa , Abu Hamdeh Nizar
Published: June 2024

Antibiotic resistance among bacteria is recognized as the primary factor contributing to the failure of treatment. In this research, our objective was to examine the prevalence of antibiotic resistance in H. pylori bacteria in Palestine. We enlisted 91 individuals suffering from dyspepsia, comprising 49 females and 42 males. These participants underwent esophagogastroduodenoscopy procedures with gastric biopsies. These biopsies were subsequently subjected to microbiological assessments and tested for their susceptibility to various antimicrobial drugs. Among the 91 patients, 38 (41.7%) exhibited the presence of H. pylori. Notably, Ciprofloxacin displayed the highest efficacy against H. pylori, followed by Levofloxacin, Moxifloxacin, and Amoxicillin, with resistance rates of 0%, 0%, 2.6%, and 18.4%, respectively. On the contrary, Metronidazole and Clarithromycin demonstrated the lowest effectiveness, with resistance percentages of 100% and 47.4%, respectively. The outcomes of this investigation emphasize that H. pylori strains within the Palestinian patient group exhibit substantial resistance to conventional first-line antibiotics like clarithromycin and metronidazole. However, alternative agents such as fluoroquinolones and amoxicillin remain efficacious choices. Consequently, we recommend favoring quinolone-based treatment regimens for H. pylori infections and adopting a more judicious approach to antibiotic usage among the Palestinian population.
RNAi-mediated downregulation of AcCENH3 can induce in vivo haploids in onion (Allium cepa L.)
Authors: Manape Tushar K. , Satheesh Viswanathan , Somasundaram Saravanakumar , Soumia Parakkattu S. , Khade Yogesh P. , Mainkar Pawan , Mahajan Vijay , Singh Major , Anandhan Sivalingam
Published: June 2024

Haploid induction (HI) holds great promise in expediting the breeding process in onion, a biennial cross-pollinated crop. We used the CENH3-based genome elimination technique in producing a HI line in onion. Here, we downregulated AcCENH3 using the RNAi approach without complementation in five independent lines. Out of five events, only three could produce seeds upon selfing. The progenies showed poor seed set and segregation distortion, and we were unable to recover homozygous knockdown lines. The knockdown lines showed a decrease in accumulation of AcCENH3 transcript and protein in leaf tissue. The decrease in protein content in transgenic plants was correlated with poor seed set. When the heterozygous knockdown lines were crossed with wild-type plants, progenies showed HI by genome elimination of the parental chromosomes from AcCENH3 knockdown lines. The HI efficiency observed was between 0 and 4.63% in the three events, and it was the highest (4.63%) when E1 line was crossed with wildtype. Given the importance of doubled haploids in breeding programmes, the findings from our study are poised to significantly impact onion breeding.
Blockage of Akt activation suppresses cadmium-induced renal tubular cellular damages through aggrephagy in HK-2 cells
Authors: Fujiki Kota , Tanabe K. , Suzuki S. , Mochizuki A. , Mochizuki-Kashio M. , Sugaya T. , Mizoguchi T. , Itoh M. , Nakamura-Ishizu A. , Inamura H. , Matsuoka M.
Published: June 2024

We have reported that an environmental pollutant, cadmium, promotes cell death in the human renal tubular cells (RTCs) through hyperactivation of a serine/threonine kinase Akt. However, the molecular mechanisms downstream of Akt in this process have not been elucidated. Cadmium has a potential to accumulate misfolded proteins, and proteotoxicity is involved in cadmium toxicity. To clear the roles of Akt in cadmium exposure-induced RTCs death, we investigated the possibility that Akt could regulate proteotoxicity through autophagy in cadmium chloride (CdCl2)-exposed HK-2 human renal proximal tubular cells. CdCl2 exposure promoted the accumulation of misfolded or damaged proteins, the formation of aggresomes (pericentriolar cytoplasmic inclusions), and aggrephagy (selective autophagy to degrade aggresome). Pharmacological inhibition of Akt using MK2206 or Akti-1/2 enhanced aggrephagy by promoting dephosphorylation and nuclear translocation of transcription factor EB (TFEB)/transcription factor E3 (TFE3), lysosomal transcription factors. TFEB or TFE3 knockdown by siRNAs attenuated the protective effects of MK2206 against cadmium toxicity. These results suggested that aberrant activation of Akt attenuates aggrephagy via TFEB or TFE3 to facilitate CdCl2-induced cell death. Furthermore, these roles of Akt/TFEB/TFE3 were conserved in CdCl2-exposed primary human RTCs. The present study shows the molecular mechanisms underlying Akt activation that promotes cadmium-induced RTCs death.