bluuebunny/crossref_metadata_2025 huggingface dataset:
The Central American Power System: Achievements, Challenges, and Opportunities for a Green Transition
Authors: Gómez-Ramírez Gustavo Adolfo , Meza Carlos , Mora-Jiménez Gonzalo , Morales José Rodrigo Rojas , García-Santander Luis
Published: May 2023

Over the past few decades, Central American countries have seen a steady increase in their energy needs. Luckily, the region has abundant renewable energy resources and, as a result, has been busy constructing wind and photovoltaic power facilities. However, while these renewable sources are promising, they come with some risks—mainly, their variable power generation can pose a challenge to the interconnected regional system. This paper explores the current state of the Central American power system and the obstacles it faces as it strives to transition to a more environmentally-friendly energy system. To do so, the authors employed power flow analysis and transient stability studies, which were conducted using ETAP (Electrical Transient Analyzer Program) to model and simulate the power system. Their study revealed that the Central American power system is at risk of instability, and they suggest that integrating ancillary services and storage solutions could strengthen its resilience. Additionally, the authors advocate for the development of microgrids, energy management, and sustainable decarbonization plans. Lastly, the authors emphasize the importance of short-, medium-, and long-term power planning to make better decisions.
Maximum Power Point Tracking of Photovoltaic Module Arrays Based on a Modified Gray Wolf Optimization Algorithm
Authors: Huang Kuo-Hua , Chao Kuei-Hsiang , Kuo Ying-Piao , Chen Hong-Han
Published: May 2023

In this study, a modified gray wolf optimization algorithm (GWOA) was proposed to facilitate the maximum power point tracking (MPPT) of photovoltaic module arrays (PMAs). To increase the voltage conversion ratio and achieve a voltage boost through reduced duty cycles, a high-voltage step-up converter with a coupled inductor was used to replace the conventional energy storage inductor. To achieve global MPPT, the iteration parameters of the proposed GWOA were adjusted according to the slope of the PMA power–voltage (P–V) curve. According to the simulation results, the modified GWOA is more effective in MPPT than the perturbation and observation algorithm and conventional GWOA when multiple peaks appear in the P–V curve of a shaded PMA. In addition, the modified GWOA exhibits an improved tracking speed response and steady-state response.
A Review of Bionic Structures in Control of Aerodynamic Noise of Centrifugal Fans
Authors: Zhou Wenqiang , Zhou Peijian , Xiang Chun , Wang Yang , Mou Jiegang , Cui Jiayi
Published: May 2023

Due to the complexity of the working conditions and the diversity of application scenarios, the normal operation of a fan, whether volute tongue, volute shell surface, or blade, often encounters some unavoidable problems, such as flow separation, wear, vibration, etc.; the aerodynamic noise caused by these problems has a significant impact on the normal operation of the fan. However, despite the use of aerodynamic acoustics to design low-noise fans or the use of sound absorption, sound insulation, and sound dissipation as the main traditional noise control techniques, they are in a state of technical bottleneck. Thus, the search for more efficient methods of noise reduction is looking toward the field of bionics. For this purpose, this paper first analyzes the mechanism of fan noise in the volute tongue and blades, and then, this paper reviews the noise control mechanism and improvement research using the bionic structures in the volute tongue structure, the contact surface of the volute shell, and the leading and trailing edges of the blade in the centrifugal fan. Finally, the current challenges and prospects of bionic structures for aerodynamic noise control of centrifugal fans are discussed.
Performance Enhancement of Photovoltaic-Thermal Modules Using a New Environmentally Friendly Paraffin Wax and Red Wine-rGO/H2O Nanofluid
Authors: Nabi Hossein , Gholinia Mosayeb , Khiadani Mehdi , Shafieian Abdellah
Published: May 2023

Photovoltaic/thermal systems are one of the most efficient types of solar collectors because they absorb solar radiation and generate electricity and heat simultaneously. For the first time, this paper presents an investigation into the impact of red wine-rGO/H2O nanofluid and paraffin wax on the thermohydraulic properties of a photovoltaic/thermal system. The study focuses on three innovative nonlinear arrangements of the serpentine tubes. The effects of these materials and configurations are analyzed through numerical simulations. To improve the performance, environmentally friendly materials, including red wine-rGO/H2O nanofluid and paraffin wax, have been used. Various performative parameters such as electrical and thermal efficiency of the photovoltaic/thermal system, exergy, and nanofluid concentration were investigated. The results demonstrated a significant enhancement in the system’s performance when using innovative serpentine tubes instead of simple tubes for the fluid flow path. The use of paraffin C18 increases electrical efficiency, while the use of paraffin C22 improves thermal efficiency. Moreover, the incorporation of phase change materials along with the utilization of innovative geometries in the serpentine tube led to a notable improvement in the outlet temperature of the fluid, increasing it by 2.43 K. Simultaneously, it substantially reduced the temperature of the photovoltaic cells, lowering it by 21.55 K. In addition, the new model demonstrated significant improvements in both thermal and electrical efficiency compared to the simple model. Specifically, the maximum thermal efficiency improvement reached 69.2%, while the maximum electrical efficiency improvement reached 11.7%.
Influence of Nickel Loading and the Synthesis Method on the Efficiency of Ni/TiO2 Catalysts for Renewable Diesel Production
Authors: Petropoulos George , Zafeiropoulos John , Kordouli Eleana , Lycourghiotis Alexis , Kordulis Christos , Bourikas Kyriakos
Published: May 2023

The efficiency of Ni/TiO2 catalysts for renewable diesel production was evaluated in the present study. Two series of catalysts were synthesized and characterized using various physicochemical techniques (N2 physisorption, XRD, SEM, XPS, H2-TPR, and NH3–TPD). In the first series of catalysts, successive dry impregnations (SDI) were used for depositing 10, 20, 30, 50, and 60 wt.% Ni. The yield towards renewable diesel is maximized over the catalyst with 50 wt.% Ni loading. Selecting this optimum loading, a second series of catalysts were synthesized via three additional preparation methods: wet impregnation (WI) and deposition–precipitation using either ammonia (DP-NH3) or urea (DP-Urea) as the precipitation agent. The catalysts’ efficiency in the production of green diesel is influenced by the preparation method following the order: DP-Urea > DP-NH3 > WI ≈ SDI. The metallic surface area and the balanced acidity mainly determine the performance of the catalysts.
A Mass Balance-Based Method for the Anaerobic Digestion of Rice Straw
Authors: Bressan Maurizio , Campagnoli Elena , Ferro Carlo Giovanni , Giaretto Valter
Published: May 2023

Current rice straw disposal practices have serious repercussions on the environment and, in addition, do not consider its energy potential. On the contrary, the anaerobic digestion of rice straw makes it possible to produce renewable energy and to reintroduce into the soil the nutrients present in the digestate, at the same time, reducing greenhouse gas emissions from paddies. For rice straw of different geographical origin, by applying a mass balance method to the digester, the minimum requirements in terms of conditioners (nitrogen, phosphorus and potassium) and water, which allow obtaining the maximum production of methane, were calculated. The results obtained show that after the first 30 days (hydraulic retention time) for each ton of rice straw digested, the daily water consumption varies considerably from one country to another, from a minimum value of 1.5 m3/d to a maximum of 4.3 m3/d. After the same time, the addition of nitrogen and phosphorus is only required for the optimal anaerobic digestion of Indian rice straw. The low presence of these elements in Indian straw requires an addition of 3 kg/d of urea and 1.5 kg/d of superphosphate to compensate for the lack of nitrogen and phosphorus, respectively. In all the examined cases, the concentration of potassium, even if higher than the optimal value, does not reach levels that can significantly affect the methane production.
A MILP Model for Optimal Conductor Selection and Capacitor Banks Placement in Primary Distribution Systems
Authors: Gallego Pareja Luis A. , López-Lezama Jesús M. , Gómez Carmona Oscar
Published: May 2023

Power distribution systems (PDS) are the infrastructure and equipment used to distribute electricity from the transmission system to end-users, such as homes and businesses. PDS are usually designed to operate in a radial mode, where power flows from one substation to the end user through a series of feeders. The extension of distribution lines to attend new customers along with the growing demand for electricity result in increased energy losses and voltage reductions. Various solutions have been proposed to solve these issues, such as selecting the optimal set of conductors, optimizing the placement of voltage regulators, using capacitor banks, reconfiguring the distribution system, and implementing distributed generation. A well-known approach for reducing energy losses and enhancing voltage profile is the optimal conductor selection (OCS). While this can be beneficial, it may not be sufficient to fully reduce technical losses and improve the system voltage profile; therefore, it must be combined with other strategies. This paper presents a new approach that combines the OCS with the optimal placement of capacitor banks (OPCB) to minimize technical losses and improve the voltage profile in PDS. The main contribution of this paper is the integration of these two problems into a single mixed integer linear programming (MILP) model, therefore guaranteeing the achievement of globally optimal solutions. Three test systems of 27, 69, and 85 buses were used to illustrate the effectiveness of the proposed modeling approach. The results indicate that the combination of OCS and OPCB effectively minimizes energy losses and enhances the voltage profile. In all cases, the solutions obtained by the proposed MILP approach were better than those previously reported through metaheuristics for the combined OCS and OPCB problem.
Simulation Study on Heating Stability of PV/T-GSHP Automatic Control Heating System Based on TRNSYS
Authors: Zhao Jianhui , Chen Jiamei , Liu Panxue
Published: May 2023

The PV/T-GSHP heating control system is proposed to address the energy consumption problem of building heating in northern China. By using TRNSYS to establish an automatic control model, the control of the heat pump start/stop, water supply temperature on the load side of the tank, and high temperature water return to the building can achieve the purpose of being energy saving, providing stable heating, and providing control of high temperature heating. The results show that the PV/T-GSHP control system has an annual fluctuation range of 0.01~0.17 kW in the heating period for a typical building heat supply and building heat load, and the system energy efficiency and energy consumption are increased by 14.5% and 33.91%, respectively, compared with the GSHP system, and the annual value of cost is reduced by 8.49% and 19.09% compared with the GSHP and traditional coal-fired boiler system, which shows that the PV/T-GSHP control system is more economical. It can be seen that the PV/T-GSHP control system has better heating stability, energy efficiency, and economy, and it provides theoretical significance for the current clean heating automatic control scheme in Chinese northern areas.
Thermal–Hydraulic Calculation and Analysis on Water Wall System of a 700 MWe Ultra-Supercritical CFB Boiler
Authors: Wu Haowen , Zhou Tuo , Hu Xiannan , Luo Yongjun , Zhang Man , Yang Hairui
Published: May 2023

Ultra-supercritical circulating fluidized bed (CFB) boiler combustion technology has the advantages of environmental protection and good commercialization. As one of the key aspects in ultra-supercritical CFB combustion technology, the water wall system directly determines whether the CFB boiler can safely and effectively transform heat energy. This paper studies the thermal–hydraulic characteristics of the designed water wall system of a 700 MWe ultra-supercritical CFB boiler, the largest one that will be built next year. At four loads of the water wall system, hydrodynamic parameters are calculated based on the successfully verified mathematical models. The results show that the hydrodynamic characteristics of the system are good. The temperature distribution of the working fluid and metal of the water wall system is acceptable and safe at each load, which can support the technical development and improvement of the 700 MWe ultra-supercritical CFB boiler.
Soil Depth Can Modify the Contribution of Root System Architecture to the Root Decomposition Rate
Authors: Tang Yingzhou , Liu Xin , Lian Jingwei , Cheng Xuefei , Wang G. Geoff , Zhang Jinchi
Published: May 2023

Aims: Changes in root system architecture (RSA) and soil depth affect the root decomposition rate. However, due to soil opacity, many variables of RSA have not been well studied or even measured. Methods: To investigate the effects of soil depth and the characteristics of RSA on the root decomposition rate, soil samples (Soil cores were collected in October 2020 from Cunninghamia lanceolata and Pinus taeda plantations, which were 40 years old) were obtained using a soil auger and had a diameter of 10 cm and a length of 60 cm. Samples were taken from six different soil depths, ranging from 0 to 60 cm with a 10 cm interval between each depth. The RSA in the in-situ soil cores was analyzed using computed tomography scans and Avizo. Results: Root volume and the number of root throats were significantly higher at the 0–10 cm soil depth than at the 10–60 cm soil depth, but root length was significantly lower at the 50–60 cm soil depth (p < 0.05). Structural equation modeling showed that different stand types influenced root biomass and thus the root decomposition rate directly or indirectly through the characteristics of the stand types. RSA, i.e., root thickness and breadth, affected root biomass indirectly and then affected the root decomposition rate. Root biomass contributed the most to the root decomposition rate in the Cunninghamia lanceolata (20.19%) and Pinus taeda (32.26%) plantations. The contribution of the RSA variables to the root decomposition rate exceeded 50% at the 20–30 cm and 40–50 cm soil depths. Conclusions: Our findings suggested that the influence of the RSA variables on the root decomposition rate varies with soil depth. This deserves more consideration in our future studies on root decomposition and RSA.
Fluorescence Properties of Pterocarpus Wood Extract
Authors: Li Renjie , Li Junyuan , Shi Jiangtao , Zhang Yongyue , Sun Yuxin , Chen Yuxi , Liu Zhipeng
Published: May 2023

The water immersion of Pterocarpus wood produces strong blue fluorescence, which comes from the extract. The fluorescence contained in the extract is of interest for the identification of Pterocarpus wood. We conducted an investigation into the extraction solution of Pterocarpus wood and analyzed the mechanism of fluorescence in this species. Possible species of the fluorescent molecules are discussed based on the mixture. Liquid chromatography mass spectrometry (LC-MS) is used for an analysis of the extract, the obtained substances that may be fluorescent in Pterocarpus wood. In addition, the change in the fluorescence intensity with changes in the pH and concentration in the extract is also studied. The results show that the fluorescent molecule is quenched by aggregation (Aggregation-Caused Quenching; ACQ) and is unstable in over-acidic and over-alkaline conditions (especially acidic).
Effects of Deciduous Forests on Adolescent Emotional Health in Urban Areas: An Example from the Autumn Ginkgo Forest in Chengdu
Authors: Wu Linjia , Luo Shixian , Li Di , Chen Qibing , Li Jingting , Wen Jiacai
Published: May 2023

Many studies have shown that urban green spaces can promote emotional health. Deciduous forest is an important landscape and ecological resource of the city. However, the emotional impact of different behavior patterns in this kind of natural space with obvious seasonal changes are rarely discussed. This study explores the emotional feedback of two typical behavior patterns within an urban landscape forest in autumn: sitting and walking. We recruited 80 volunteers and divided them into a sitting viewing group (group S) and a walking viewing group (group W). On the premise of gender balance, they were randomly assigned to a natural path under a Ginkgo biloba forest in autumn for 15 min of viewing. Physiological and psychological indicators were used for monitoring. Blood pressure, heart rate and electroencephalography (EEG) were used for physiological indicators, and a POMS questionnaire was used for psychological indicators. A paired t-test and one-way ANOVA were used to analyze the physiological parameters of the two experimental groups, and a paired Wilcoxon signed-rank sum test was used to analyze the differences in psychological indexes between the two behavior groups. The results showed that the diastolic blood pressure and pulse of the sitting group decreased significantly under the seasonal deciduous forest, and the walking group attained higher “Engagement”, “Excitement”, and “Relaxation”. In the absolute α and β waves, there were significant differences in the parietal P8 channels between the sitting and walking groups, and significant differences in the RAB indicators of the AF3, F7, P7, FC5, FC6, F3 and T7 channels. The two behavior patterns can effectively reduce negative mood, and the “Vigor” mood in the walking group was significantly increased, which was significantly better than that of the sitting group in reducing negative mood. These results enrich research on the influence on emotional health in the field of seasonal green-space restoration. The differences of different behavior patterns can provide guidance for planning urban landscape forest construction and activity facilities.
Structural Characterization of Trivalvaria costata Chloroplast Genome and Molecular Evolution of rps12 Gene in Magnoliids
Authors: Ping Jingyao , Zhong Xiaona , Wang Ting , Su Yingjuan
Published: May 2023

Chloroplasts (cp) are important organelles in plant cells that have been widely used in phylogenetic, molecular evolution, and gene expression studies due to their conserved molecular structure. In this study, we obtained the complete cp genome of Trivalvaria costata (Annonaceae) and analyzed its structural characteristics. Additionally, we analyzed the rps12 gene in the phylogenetic framework of magnoliids. The T. costata cp genome comprises 162,002 bp and contains 132 genes. We detected 48 simple sequence repeats (SSRs) and identified 29 high-frequency codons as well as 8 optimal codons. Our multiple analyses show that codon usage bias is mainly influenced by natural selection. For the first time, we found the rps12 gene to be entirely located in the IR region (in Annona). In groups with exon 1 located in the single-copy (SC) region and exons 2–3 located in the inverted repeat (IR) region, the transition rate and synonymous substitution rate of exon 1 were higher than those of exons 2–3. Adaptive evolution identified a positive selection site (116) located in the 310-helix region, suggesting that the rps12 gene may undergo adaptive changes during the evolutionary history of magnoliids. This study enhances our knowledge regarding genetic information on T. costata and provides support for reduced substitution rates in the IR region.
A Comparison of Four Spatial Interpolation Methods for Modeling Fine-Scale Surface Fuel Load in a Mixed Conifer Forest with Complex Terrain
Authors: Hoffman Chad M. , Ziegler Justin P. , Tinkham Wade T. , Hiers John Kevin , Hudak Andrew T.
Published: May 2023

Patterns of spatial heterogeneity in forests and other fire-prone ecosystems are increasingly recognized as critical for predicting fire behavior and subsequent fire effects. Given the difficulty in sampling continuous spatial patterns across scales, statistical approaches are common to scale from plot to landscapes. This study compared the performance of four spatial interpolation methods (SIM) for mapping fine-scale fuel loads: classification (CL), multiple linear regression (LR), ordinary kriging (OK), and regression kriging (RK). These methods represent commonly used SIMs and demonstrate a diversity of non-geostatistical, geostatistical, and hybrid approaches. Models were developed for a 17.6-hectare site using a combination of metrics derived from spatially mapped trees, surface fuels sampled with an intensive network of photoload plots, and topographic variables. The results of this comparison indicate that all estimates produced unbiased spatial predictions. Regression kriging outperformed the other approaches that either relied solely on interpolation from point observations or regression-based approaches using auxiliary information for developing fine-scale surface fuel maps. While our analysis found that surface fuel loading was correlated with species composition, forest structure, and topography, the relationships were relatively weak, indicating that other variables and spatial interactions could significantly improve surface fuel mapping.
Computed Tomography-Assisted Study of the Liquid Contrast Agent’s Spread in a Hydrogel Phantom of the Brain Tissue
Authors: Vanina Anastasia S. , Sychev Alexander V. , Lavrova Anastasia I. , Gavrilov Pavel V. , Andropova Polina L. , Grekhnyova Elena V. , Kudryavtseva Tatiana N. , Postnikov Eugene B.
Published: May 2023

Studying transport processes in the brain’s extracellular space is a complicated problem when considering the brain’s tissue. Tests of corresponding physical and mathematical problems, as well as the need for materials with cheap but realistic properties to allow for testing of drug delivery systems, lead to the development of artificial phantom media, one kind of which is explored in this work. We report results from quantifying the spread of a standard contrast agent used in clinical computed tomography, Iopromide, in samples of collagen-based hydrogels. Its pure variant as well as samples supplied with lipid and surfactant additives were explored. By comparing to solutions of the diffusion equation which reproduce these data, the respective diffusion coefficients were determined. It was shown that they are relevant to the range typical for living tissue, grow with elevation in the lipid content and diminish with growth in surfactant concentration.
Vitamin D Content in Commonly Consumed Mushrooms in Thailand and Its True Retention after Household Cooking
Authors: Sridonpai Piyanut , Suthipibul Philipda , Boonyingsathit Konpong , Chimkerd Chanika , Jittinandana Sitima , Judprasong Kunchit
Published: May 2023

This study investigated the vitamin D level of nine species of cultivated mushrooms and three species of wild mushrooms commonly consumed in Thailand and the effect of cooking on their vitamin D content. Cultivated mushrooms were obtained from three wholesale markets, while wild mushrooms were collected from three trails in a conservation area. Mushrooms from each source were separated into four groups: raw, boiled, stir-fried, and grilled. Different forms of vitamin D were analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS). The analyzed method demonstrated good linearity, accuracy, and precision, as well as being low in the limit of detection and limit of quantitation. Results showed that vitamin D2 and ergosterol (provitamin D2) were the major forms of vitamin D found in the mushrooms. Both raw cultivated and wild mushrooms had wide ranging ergosterol contents (7713–17,273 μg/100 g edible portion, EP). Lung oyster mushroom and termite mushroom contained high levels of vitamin D2 (15.88 ± 7.31 and 7.15 ± 0.67 μg/100 g EP, respectively), while other mushroom species had negligible amounts (0.06 to 2.31 μg per 100 g EP). True retention (TR) levels of vitamin D2 after boiling, stir-frying, and grilling were not significantly different (p > 0.05) (with estimated marginal means ± standard error 64.0 ± 2.3%, 58.8 ± 2.3%, and 64.7 ± 3.6% TR, respectively). Consuming cooked lung oyster mushrooms, in particular, along with regular exposure to sunlight should be promoted to reduce the incidence of vitamin D deficiency.
The Questionable Quality Profile of Food Supplements: The Case of Red Yeast Rice Marketed Products
Authors: Vitiello Antonella , Izzo Luana , Castaldo Luigi , d’Angelo Ivana , Ungaro Francesca , Miro Agnese , Ritieni Alberto , Quaglia Fabiana
Published: May 2023

Food supplements (FS) containing red yeast rice (RYR) are largely employed to reduce lipid levels in the blood. The main ingredient responsible for biological activity is monacolin K (MoK), a natural compound with the same chemical structure as lovastatin. Concentrated sources of substances with a nutritional or physiological effect are marketed in “dose” form as food supplements (FS). The quality profile of the “dosage form” of FS is not defined in Europe, whereas some quality criteria are provided in the United States. Here, we evaluate the quality profile of FS containing RYR marketed in Italy as tablets or capsules running two tests reported in The European Pharmacopoeia 11 Ed. and very close to those reported in the USP. The results highlighted variations in dosage form uniformity (mass and MoK content) compliant with The European Pharmacopoeia 11 Ed. specifications, whereas the time needed for disintegrating tablets was longer for 44% of the tested samples. The bioaccessibility of MoK was also investigated to obtain valuable data on the biological behaviour of the tested FS. In addition, a method for citrinin (CIT) determination was optimized and applied to real samples. None of the analyzed samples demonstrated CIT contamination (LOQ set at 6.25 ng/mL). Considering the widespread use of FS, our data suggest that greater attention should be paid by fabricants and regulatory authorities to ensure the quality profile and the safe consumption of marketed products.
Survey of Colistin Resistance in Commensal Bacteria from Penaeus vannamei Farms in China
Authors: Zhang Yilin , Lv Xinrui , Cao Weiwei , Zhang Huang , Shi Lei , Bai Weibin , Ye Lei
Published: May 2023

Aquatic environments are important reservoirs for drug resistance. Aquatic foods may act as carriers to lead antibiotic-resistant commensal bacteria into the human gastrointestinal system, then contacting gut microbiota and spreading antibiotic resistance. Here, several shrimp farms were investigated to identify colistin resistance among commensal bacteria of aquaculture. A total of 884 (41.6%) colistin-resistant isolates were identified among 2126 strains. Electroporation demonstrated that colistin-resistant fragments were present in some commensal bacteria that could be transferred to other bacteria. Most of the resistant bacteria were Bacillus spp., with 69.3% of the Bacillus species exhibiting multiple drug resistance. Bacillus licheniformis was prevalent, with 58 strains identified that comprised six sequence types (ST) based on multilocus sequence typing. Whole-genome sequencing and comparisons with previous B. licheniformis genomes revealed a high degree of genomic similarity among isolates from different regions. Thus, this species is widely distributed, and this study provides new insights into global antibiotic-resistant characteristics of B. licheniformis. Sequence analyses further revealed some of these strains are even pathogenic and virulent, suggesting the antibiotic resistance and hazards of commensal bacteria in aquaculture should be considered. Considering the “One Health” perspective, improved monitoring of aquatic food is needed to prevent the spread of drug-resistant commensal bacteria from food-associated bacteria to humans.
Efficient Solution of Fractional System Partial Differential Equations Using Laplace Residual Power Series Method
Authors: Shafee Ahmad , Alkhezi Yousuf , Shah Rasool
Published: May 2023

In this paper, we present an efficient solution method for solving fractional system partial differential equations (FSPDEs) using the Laplace residual power series (LRPS) method. The LRPS method is a powerful technique for solving FSPDEs, as it allows for the efficient computation of the solution in the form of a power series expansion. The method is based on the Laplace transform and the residual power series, and is applied to a system of coupled FSPDEs. The method is validated using several test problems, and the results show that the LRPS method is a reliable and efficient method for solving FSPDEs.
Identification and Functional Analysis of Transcriptome Profiles, Long Non-Coding RNAs, Single-Nucleotide Polymorphisms, and Alternative Splicing from the Oocyte to the Preimplantation Stage of Sheep by Single-Cell RNA Sequencing
Authors: Zhang Zijing , Shi Qiaoting , Zhu Xiaoting , Jin Lei , Lang Limin , Lyu Shijie , Xin Xiaoling , Huang Yongzhen , Yu Xiang , Li Zhiming , Chen Sujuan , Xu Zhaoxue , Zhang Wei , Wang Eryao
Published: May 2023

Numerous dynamic and complicated processes characterize development from the oocyte to the embryo. However, given the importance of functional transcriptome profiles, long non-coding RNAs, single-nucleotide polymorphisms, and alternative splicing during embryonic development, the effect that these features have on the blastomeres of 2-, 4-, 8-, 16-cell, and morula stages of development has not been studied. Here, we carried out experiments to identify and functionally analyze the transcriptome profiles, long non-coding RNAs, single-nucleotide polymorphisms (SNPs), and alternative splicing (AS) of cells from sheep from the oocyte to the blastocyst developmental stages. We found between the oocyte and zygote groups significantly down-regulated genes and the second-largest change in gene expression occurred between the 8- and 16-cell stages. We used various methods to construct a profile to characterize cellular and molecular features and systematically analyze the related GO and KEGG profile of cells of all stages from the oocyte to the blastocyst. This large-scale, single-cell atlas provides key cellular information and will likely assist clinical studies in improving preimplantation genetic diagnosis.
Genome-Wide Tissue-Specific Genes Identification for Novel Tissue-Specific Promoters Discovery in Soybean
Authors: Yu Lili , Zhang Hao , Guan Rongxia , Li Yinghui , Guo Yong , Qiu Lijuan
Published: May 2023

Promoters play a crucial role in controlling the spatial and temporal expression of genes at transcriptional levels in the process of higher plant growth and development. The spatial, efficient, and correct regulation of exogenous genes expression, as desired, is the key point in plant genetic engineering research. Constitutive promoters widely used in plant genetic transformation are limited because, sometimes, they may cause potential negative effects. This issue can be solved, to a certain extent, by using tissue-specific promoters. Compared with constitutive promoters, a few tissue-specific promoters have been isolated and applied. In this study, based on the transcriptome data, a total of 288 tissue-specific genes were collected, expressed in seven tissues, including the leaves, stems, flowers, pods, seeds, roots, and nodules of soybean (Glycine max). KEGG pathway enrichment analysis was carried out, and 52 metabolites were annotated. A total of 12 tissue-specific genes were selected via the transcription expression level and validated through real-time quantitative PCR, of which 10 genes showed tissue-specific expression. The 3-kb 5′ upstream regions of ten genes were obtained as putative promoters. Further analysis showed that all the 10 promoters contained many tissue-specific cis-elements. These results demonstrate that high-throughput transcriptional data can be used as effective tools, providing a guide for high-throughput novel tissue-specific promoter discovery.
Molecular Mechanisms and Mediators of Hepatotoxicity Resulting from an Excess of Lipids and Non-Alcoholic Fatty Liver Disease
Authors: Finelli Carmine
Published: May 2023

The paper reviews some of the mechanisms implicated in hepatotoxicity, which is induced by an excess of lipids. The paper spans a wide variety of topics: from the molecular mechanisms of excess lipids, to the therapy of hyperlipidemia, to the hepatotoxicity of lipid-lowering drugs. NAFLD is currently the leading cause of chronic liver disease in Western countries; the molecular mechanisms leading to NAFLD are only partially understood and there are no effective therapeutic interventions. The prevalence of liver disease is constantly increasing in industrialized countries due to a number of lifestyle variables, including excessive caloric intake, unbalanced diet, lack of physical activity, and abuse of hepatotoxic medicines. Considering the important functions of cell death and inflammation in the etiology of the majority, if not all, liver diseases, one efficient therapeutic treatment may include the administration of hepatoprotective and anti-inflammatory drugs, either alone or in combination. Clinical trials are currently being conducted in cohorts of patients with different liver diseases in order to explore this theory.
Physical Activity in Immersive Virtual Reality: A Scoping Review
Authors: Giakoni-Ramírez Frano , Godoy-Cumillaf Andrés , Espoz-Lazo Sebastián , Duclos-Bastias Daniel , del Val Martín Pablo
Published: May 2023

Physical activity has benefits for health, but many adolescents are inactive. However, video games such as Immersive Virtual Reality (IVR) have grown in popularity as a leisure activity among young people, allowing them to manipulate objects in virtual environments increasing the practice of physical activity. The evidence indicates that the interest in physical activity through IVR is greater than in traditional methods, and different experiences have been reported. However, few studies indicate the sample evaluated, the effects found, or the IVR instruments used. Due to this, is the aim of this study is to identify the publications referring to IVR and physical activity, characterize them, and present the obtained main findings. For this, the guidelines described in the PRISMA-ScR for scoping reviews were applied. After the use of the inclusion and exclusion criteria, eight articles were included. Results show evidence regarding physiological outcomes, perceptual variables, interest and enjoyment, and psychological effects regarding physical activity through IVR. Additionally, the use of different devices and their prescriptions are explored. It is concluded that there is interest from the scientific community for the practice of physical activity through IVR, as well as for its application for the maintenance of active habits. This is important as it positions IVR as a method that can be a more experiential and effective way to develop and maintain a healthy lifestyle.
Effects of Using a Shoulder/Scapular Brace on the Posture and Muscle Activity of Healthy University Students during Prolonged Typing—A Randomized Controlled Cross-Over Trial
Authors: Leung Melissa , Kan Mandy M. P. , Cheng Hugo M. H. , De Carvalho Diana E. , Anwer Shahnawaz , Li Heng , Wong Arnold Y. L.
Published: May 2023

Laptop use appears to contribute to poor working postures and neck pain among university students. Postural braces have the potential to improve upper back/neck posture and therefore might have a role as an ergonomic aid for this population. Therefore, the purpose of this study was to assess the short-term effects of scapular bracing on pain, fatigue, cervicothoracic posture, and the activity of the neck and upper-back muscles in healthy college students. A randomized controlled crossover trial was conducted to evaluate the self-reported pain and fatigue, the amplitude and median frequency of surface electromyography in neck extensors, upper trapezius, and lower trapezius, as well as the neck and shoulder sagittal alignment (measured by inertial sensors and digital photographs) during a 30-min typing task in a sample of young, healthy university students with or without a scapular brace. The brace condition resulted in significantly smaller levels of bilateral trapezius muscle activity (p < 0.01). Rounded shoulder posture was slightly better in the brace condition, but these differences were not significant (p > 0.05). There were no significant immediate differences in pain or fatigue scores, neck alignment, or the electromyographic activity of the other muscles tested between brace and non-brace conditions (all p > 0.05). However, bracing appears to immediately reduce the electromyographic activity of the lower trapezius muscles (p < 0.05). These findings shed some light on the possible advantages of scapular bracing for enhancing laptop ergonomics in this group of individuals. Future studies are warranted to evaluate the effects of different types of braces, the importance of matching the brace to the user, and the short- and long-term effects of brace use on computer posture and muscle activity.
Exploring E-Health Literacy and Technology-Use Anxiety among Older Adults in Korea
Authors: Kim Jiyoun , Jeon Sang-Wan , Byun Hyun , Yi Eunsurk
Published: May 2023

The COVID-19 pandemic has increased the importance of health literacy in disseminating information on health in a non-contact society. This study focused on examining the acceptance capacity by older adults of smart devices in Korea and investigating the potential differences between men and women in terms of e-health literacy and technology-use anxiety. The study included 1369 respondents who were adults over 50 years of age and used welfare centers, public health centers, senior citizen centers, and exercise centers in Seoul and Incheon. An online survey was conducted from 1 June 2021 to 24 June 2021. The study found that the older adults’ low levels of digital literacy could limit their access to health information and negatively impact their health. The difference between men and women in terms of technology-use anxiety was statistically significant, with the latent mean for men being higher than that for women. The effect sizes of the potential mean differences were found to be at a medium level for e-health literacy and a significant level for technology-use anxiety. With Korea’s aging population and the need for the continuous management of chronic diseases among older adults, it is essential to discuss internet-based health information for disease maintenance and treatment.
The “Dedicated” C.B.C.T. in Dentistry
Authors: Distefano Salvatore , Cannarozzo Maria Grazia , Spagnuolo Gianrico , Bucci Marco Brady , Lo Giudice Roberto
Published: May 2023

This position statement represents a consensus of an expert committee composed by the Italian Academy of General Dentistry (Accademia Italiana Odontoiatria Generale COI-AIOG) and Italian Academy of Legal and Forensic Dentistry (Accademia Italiana di Odontoiatria Legale e Forense OL-F) on the appropriate use of cone beam computed tomography (C.B.C.T.) in dentistry. This paper analyzes the use of C.B.C.T. in light of the rapid evolution of volumetric technologies, with the new low- and ultra-low-dose exposure programs. These upgrades are determining an improvement in the precision and safety of this methodology; therefore, the need of a guideline revision of the use of C.B.C.T. for treatment planning is mandatory. It appears necessary to develop a new model of use, which, in compliance with the principle of justification and as low as reasonably achievable (ALARA) and as low as diagnostically acceptable (ALADA), can allow a functional “Dedicated C.B.C.T.” exam optimized for the individuality of the patient.
Association of Insomnia with 30-Day Postpartum Readmission: A Retrospective Analysis
Authors: Kendle Anthony M. , Swanson Justin , Salemi Jason L. , Louis Judette M.
Published: May 2023

Insomnia is prevalent in pregnancy and is associated with increased use of health services. We aimed to evaluate the association between insomnia diagnosed at the delivery hospitalization and risk of 30-day postpartum readmission. We conducted a retrospective analysis of inpatient hospitalizations from the 2010–2019 Nationwide Readmissions Database. The primary exposure was a coded diagnosis of insomnia at delivery as determined by ICD-9-CM and ICD-10-CM codes. Obstetric comorbidities and indicators of severe maternal morbidity were also determined through coding. The primary outcome was all-cause 30-day postpartum readmission. Survey-weighted logistic regression was used to generate crude and adjusted odds ratios representing the association between maternal insomnia and postpartum readmission. Of over 34 million delivery hospitalizations, 26,099 (7.6 cases per 10,000) had a coded diagnosis of insomnia. People with insomnia experienced a 3.0% all-cause 30-day postpartum readmission rate, compared to 1.4% among those without insomnia. After controlling for sociodemographic, clinical, and hospital-level factors, insomnia was associated with 1.64 times higher odds of readmission (95% CI 1.47–1.83). After adjustment for obstetric comorbidity burden and severe maternal morbidity, insomnia was independently associated with 1.33 times higher odds of readmission (95% CI 1.18–1.48). Pregnant patients with insomnia have higher rates of postpartum readmission, and diagnosis of insomnia is independently associated with increased odds of readmission. Additional postpartum support may be warranted for pregnancies affected by insomnia.
First Nations, Inuit and Métis Peoples Living in Urban Areas of Canada and Their Access to Healthcare: A Systematic Review
Authors: Graham Simon , Muir Nicole M. , Formsma Jocelyn W. , Smylie Janet
Published: May 2023

In Canada, approximately 52% of First Nations, Inuit and Métis (Indigenous) peoples live in urban areas. Although urban areas have some of the best health services in the world, little is known about the barriers or facilitators Indigenous peoples face when accessing these services. This review aims to fill these gaps in knowledge. Embase, Medline and Web of Science were searched from 1 January 1981 to 30 April 2020. A total of 41 studies identified barriers or facilitators of health service access for Indigenous peoples in urban areas. Barriers included difficult communication with health professionals, medication issues, dismissal by healthcare staff, wait times, mistrust and avoidance of healthcare, racial discrimination, poverty and transportation issues. Facilitators included access to culture, traditional healing, Indigenous-led health services and cultural safety. Policies and programs that remove barriers and implement the facilitators could improve health service access for Indigenous peoples living in urban and related homelands in Canada.
MYD88 and Proinflammatory Chemokines in Aortic Atheromatosis: Exploring Novel Statin Effects
Authors: Mylonas Konstantinos S. , Peroulis Michail , Schizas Dimitrios , Kapelouzou Alkistis
Published: May 2023

Atherosclerosis is driven by a diverse range of cellular and molecular processes. In the present study, we sought to better understand how statins mitigate proatherogenic inflammation. 48 male New Zealand rabbits were divided into eight groups, each including 6 animals. The control groups received normal chow for 90 and 120 days. Three groups underwent a hypercholesterolemic diet (HCD) for 30, 60, and 90 days. Another three groups underwent HCD for 3 months, followed by normal chow for one month, with or without rosuvastatin or fluvastatin. The cytokine and chemokine expressions were assessed in the samples of thoracic and abdominal aorta. Rosuvastatin significantly reduced MYD88, CCL4, CCL20, CCR2, TNF-α, IFN-β, IL-1b, IL-2, IL-4, IL-8, and IL-10, both in the thoracic and abdominal aorta. Fluvastatin also downregulated MYD88, CCR2, IFN-β, IFN-γ, IL-1b, IL-2, IL-4, and IL-10 in both aortic segments. Rosuvastatin curtailed the expression of CCL4, IFN-β, IL-2, IL-4, and IL-10 more effectively than fluvastatin in both types of tissue. MYD88, TNF-α, IL-1b, and IL-8 showed a stronger downregulation with rosuvastatin compared to fluvastatin only in the thoracic aorta. The CCL20 and CCR2 levels reduced more extensively with rosuvastatin treatment only in abdominal aortic tissue. In conclusion, statin therapy can halt proatherogenic inflammation in hyperlipidemic animals. Rosuvastatin may be more effective in downregulating MYD88 in atherosclerotic thoracic aortas.
Updates on the Biofunctionalization of Gold Nanoparticles for the Rapid and Sensitive Multiplatform Diagnosis of SARS-CoV-2 Virus and Its Proteins: From Computational Models to Validation in Human Samples
Authors: Ionescu Rodica Elena
Published: May 2023

Since the outbreak of the pandemic respiratory virus SARS-CoV-2 (COVID-19), academic communities and governments/private companies have used several detection techniques based on gold nanoparticles (AuNPs). In this emergency context, colloidal AuNPs are highly valuable easy-to-synthesize biocompatible materials that can be used for different functionalization strategies and rapid viral immunodiagnosis. In this review, the latest multidisciplinary developments in the bioconjugation of AuNPs for the detection of SARS-CoV-2 virus and its proteins in (spiked) real samples are discussed for the first time, with reference to the optimal parameters provided by three approaches: one theoretical, via computational prediction, and two experimental, using dry and wet chemistry based on single/multistep protocols. Overall, to achieve high specificity and low detection limits for the target viral biomolecules, optimal running buffers for bioreagent dilutions and nanostructure washes should be validated before conducting optical, electrochemical, and acoustic biosensing investigations. Indeed, there is plenty of room for improvement in using gold nanomaterials as stable platforms for ultrasensitive and simultaneous “in vitro” detection by the untrained public of the whole SARS-CoV-2 virus, its proteins, and specific developed IgA/IgM/IgG antibodies (Ab) in bodily fluids. Hence, the lateral flow assay (LFA) approach is a quick and judicious solution to combating the pandemic. In this context, the author classifies LFAs according to four generations to guide readers in the future development of multifunctional biosensing platforms. Undoubtedly, the LFA kit market will continue to improve, adapting researchers’ multidetection platforms for smartphones with easy-to-analyze results, and establishing user-friendly tools for more effective preventive and medical treatments.