bluuebunny/crossref_metadata_2025 huggingface dataset:
The Unsuitability of Energy Expenditure Derived From Microtechnology for Assessing Internal Load in Collision-Based Activities
Authors: Highton Jamie , Mullen Thomas , Norris Jonathan , Oxendale Chelsea , Twist Craig
Published: May 2016

This aim of this study was to examine the validity of energy expenditure derived from microtechnology when measured during a repeated-effort rugby protocol. Sixteen male rugby players completed a repeated-effort protocol comprising 3 sets of 6 collisions during which movement activity and energy expenditure (EEGPS) were measured using microtechnology. In addition, energy expenditure was estimated from open-circuit spirometry (EEVO2). While related (r = .63, 90%CI .08–.89), there was a systematic underestimation of energy expenditure during the protocol (–5.94 ± 0.67 kcal/min) for EEGPS (7.2 ± 1.0 kcal/min) compared with EEVO2 (13.2 ± 2.3 kcal/min). High-speed-running distance (r = .50, 95%CI –.66 to .84) was related to EEVO2, while PlayerLoad was not (r = .37, 95%CI –.81 to .68). While metabolic power might provide a different measure of external load than other typically used microtechnology metrics (eg, high-speed running, PlayerLoad), it underestimates energy expenditure during intermittent team sports that involve collisions.
Repeated Sprints: An Independent Not Dependent Variable
Authors: Taylor Jonathan M. , Macpherson Tom W. , Spears Iain R. , Weston Matthew
Published: May 2016

The ability to repeatedly perform sprints has traditionally been viewed as a key performance measure in team sports, and the relationship between repeated-sprint ability (RSA) and performance has been explored extensively. However, when reviewing the repeated-sprint profile of team-sports match play it appears that the occurrence of repeated-sprint bouts is sparse, indicating that RSA is not as important to performance as commonly believed. Repeated sprints are, however, a potent and time-efficient training strategy, effective in developing acceleration, speed, explosive leg power, aerobic power, and high-intensity-running performance—all of which are crucial to team-sport performance. As such, we propose that repeated-sprint exercise in team sports should be viewed as an independent variable (eg, a means of developing fitness) as opposed to a dependent variable (eg, a means of assessing fitness/performance).
Teaching an old antibody new tricks
Authors: Gill Saar
Published: May 2016

Anti-EGFR antibodies induce immunogenic cell death in colorectal cancer.
Status of the Archaeal and Bacterial Census: an Update
Authors: Schloss Patrick D. , Girard Rene A. , Martin Thomas , Edwards Joshua , Thrash J. Cameron
Published: May 2016

A census is typically carried out for people across a range of geographical levels; however, microbial ecologists have implemented a molecular census of bacteria and archaea by sequencing their 16S rRNA genes. We assessed how well the census of full-length 16S rRNA gene sequences is proceeding in the context of recent advances in high-throughput sequencing technologies because full-length sequences are typically used as references for classification of the short sequences generated by newer technologies. Among the 1,411,234 and 53,546 full-length bacterial and archaeal sequences, 94.5% and 95.1% of the bacterial and archaeal sequences, respectively, belonged to operational taxonomic units (OTUs) that have been observed more than once. Although these metrics suggest that the census is approaching completion, 29.2% of the bacterial and 38.5% of the archaeal OTUs have been observed more than once. Thus, there is still considerable diversity to be explored. Unfortunately, the rate of new full-length sequences has been declining, and new sequences are primarily being deposited by a small number of studies. Furthermore, sequences from soil and aquatic environments, which are known to be rich in bacterial diversity, represent only 7.8 and 16.5% of the census, while sequences associated with host-associated environments represent 55.0% of the census. Continued use of traditional approaches and new technologies such as single-cell genomics and short-read assembly are likely to improve our ability to sample rare OTUs if it is possible to overcome this sampling bias. The success of ongoing efforts to use short-read sequencing to characterize archaeal and bacterial communities requires that researchers strive to expand the depth and breadth of this census.

IMPORTANCE
The biodiversity contained within the bacterial and archaeal domains dwarfs that of the eukaryotes, and the services these organisms provide to the biosphere are critical. Surprisingly, we have done a relatively poor job of formally tracking the quality of the biodiversity as represented in full-length 16S rRNA genes. By understanding how this census is proceeding, it is possible to suggest the best allocation of resources for advancing the census. We found that the ongoing effort has done an excellent job of sampling the most abundant organisms but struggles to sample the rarer organisms. Through the use of new sequencing technologies, we should be able to obtain full-length sequences from these rare organisms. Furthermore, we suggest that by allocating more resources to sampling environments known to have the greatest biodiversity, we will be able to make significant advances in our characterization of archaeal and bacterial diversity.
The Emerging Amphibian Fungal Disease, Chytridiomycosis: A Key Example of the Global Phenomenon of Wildlife Emerging Infectious Diseases
Authors: Kolby Jonathan E. , Daszak Peter
Published: May 2016

The spread of amphibian chytrid fungus,
Batrachochytrium dendrobatidis
, is associated with the emerging infectious wildlife disease chytridiomycosis. This fungus poses an overwhelming threat to global amphibian biodiversity and is contributing toward population declines and extinctions worldwide. Extremely low host-species specificity potentially threatens thousands of the 7,000+ amphibian species with infection, and hosts in additional classes of organisms have now also been identified, including crayfish and nematode worms.

Soon after the discovery of
B. dendrobatidis
in 1999, it became apparent that this pathogen was already pandemic; dozens of countries and hundreds of amphibian species had already been exposed. The timeline of
B. dendrobatidis
’s global emergence still remains a mystery, as does its point of origin. The reason why
B. dendrobatidis
seems to have only recently increased in virulence to catalyze this global disease event remains unknown, and despite 15 years of investigation, this wildlife pandemic continues primarily uncontrolled. Some disease treatments are effective on animals held in captivity, but there is currently no proven method to eradicate
B. dendrobatidis
from an affected habitat, nor have we been able to protect new regions from exposure despite knowledge of an approaching “wave” of
B. dendrobatidis
and ensuing disease.

International spread of
B. dendrobatidis
is largely facilitated by the commercial trade in live amphibians. Chytridiomycosis was recently listed as a globally notifiable disease by the World Organization for Animal Health, but few countries, if any, have formally adopted recommended measures to control its spread. Wildlife diseases continue to emerge as a consequence of globalization, and greater effort is urgently needed to protect global health.
Metalloproteinases: a Functional Pathway for Myeloid Cells
Authors: Chou Jonathan , Chan Matilda F. , Werb Zena
Published: May 2016

Myeloid cells have diverse roles in regulating immunity, inflammation, and extracellular matrix turnover. To accomplish these tasks, myeloid cells carry an arsenal of metalloproteinases, which include the matrix metalloproteinases and the adamalysins. These enzymes have diverse substrate repertoires, and are thus involved in mediating proteolytic cascades, cell migration, and cell signaling. Dysregulation of metalloproteinases contributes to pathogenic processes, including inflammation, fibrosis, and cancer. Metalloproteinases also have important nonproteolytic functions in controlling cytoskeletal dynamics during macrophage fusion and enhancing transcription to promote antiviral immunity. This review highlights the diverse contributions of metalloproteinases to myeloid cell functions.
The Phagocyte, Metchnikoff, and the Foundation of Immunology
Authors: Teti Giuseppe , Biondo Carmelo , Beninati Concetta
Published: May 2016

Since the ability of some cells to engulf particulate material was observed before Metchnikoff, he did not “discover” phagocytosis, as is sometimes mentioned in textbooks. Rather, he assigned to particle internalization the role of defending the host against noxious stimuli, which represented a new function relative to the previously recognized task of intracellular digestion. With this proposal, Metchnikoff built the conceptual framework within which immunity could finally be seen as an active host function triggered by noxious stimuli. In this sense, Metchnikoff can be rightly regarded as the father of all immunological sciences and not only of innate immunity or myeloid cell biology. Moreover, the recognition properties of his phagocyte fit surprisingly well with recent discoveries and modern models of immune sensing. For example, rather than assigning to immune recognition exclusively the function of eliminating nonself components (as others did after him), Metchnikoff viewed phagocytes as homeostatic agents capable of monitoring the internal environment and promoting tissue remodeling, thereby continuously defining the identity of the organism. No doubt, Metchnikoff’s life and creativity can provide, still today, a rich source of inspiration.
Molecular Mechanisms of Phagosome Formation
Authors: Jaumouillé Valentin , Grinstein Sergio
Published: May 2016

Phagocytosis refers to the active process that allows cells to take up large particulate material upon binding to surface receptors. The discovery of phagocytosis in 1883 by Elie Metchnikoff, leading to the concept that specialized cells are implicated in the defense against microbes, was one of the starting points of the field of immunology. After more than a century of research, phagocytosis is now appreciated to be a widely used process that enables the cellular uptake of a remarkable variety of particles, including bacteria, fungi, parasites, viruses, dead cells, and assorted debris and solid materials. Uptake of foreign particles is performed almost exclusively by specialized myeloid cells, commonly termed “professional phagocytes”: neutrophils, monocytes, macrophages, and dendritic cells. Phagocytosis of microbes not only stops or at least restricts the spread of infection but also plays an important role in regulating the innate and adaptive immune responses. Activation of the myeloid cells upon phagocytosis leads to the secretion of cytokines and chemokines that convey signals to a variety of immune cells. Moreover, foreign antigens generated by the degradation of microbes following phagocytosis are loaded onto the major histocompatibility complex for presentation to specific T lymphocytes. However, phagocytosis is not restricted to professional myeloid phagocytes; an expanding diversity of cell types appear capable of engulfing apoptotic bodies and debris, playing a critical role in tissue remodeling and in the clearance of billions of effete cells every day.
Tumor-Induced Myeloid-Derived Suppressor Cells
Authors: De Sanctis Francesco , Bronte Vincenzo , Ugel Stefano
Published: May 2016

Myeloid-derived suppressor cells (MDSCs) represent a heterogeneous, immune-suppressive leukocyte population that develops systemically and infiltrates tumors. MDSCs can restrain the immune response through different mechanisms including essential metabolite consumption, reactive oxygen and nitrogen species production, as well as display of inhibitory surface molecules that alter T-cell trafficking and viability. Moreover, MDSCs play a role in tumor progression, acting directly on tumor cells and promoting cancer stemness, angiogenesis, stroma deposition, epithelial-to-mesenchymal transition, and metastasis formation. Many biological and pharmaceutical drugs affect MDSC expansion and functions in preclinical tumor models and patients, often reversing host immune dysfunctions and allowing a more effective tumor immunotherapy.
Small RNAs in Bacterial Virulence and Communication
Authors: Svensson Sarah L. , Sharma Cynthia M.
Published: May 2016

Bacterial pathogens must endure or adapt to different environments and stresses during transmission and infection. Posttranscriptional gene expression control by regulatory RNAs, such as small RNAs and riboswitches, is now considered central to adaptation in many bacteria, including pathogens. The study of RNA-based regulation (riboregulation) in pathogenic species has provided novel insight into how these bacteria regulate virulence gene expression. It has also uncovered diverse mechanisms by which bacterial small RNAs, in general, globally control gene expression. Riboregulators as well as their targets may also prove to be alternative targets or provide new strategies for antimicrobials. In this article, we present an overview of the general mechanisms that bacteria use to regulate with RNA, focusing on examples from pathogens. In addition, we also briefly review how deep sequencing approaches have aided in opening new perspectives in small RNA identification and the study of their functions. Finally, we discuss examples of riboregulators in two model pathogens that control virulence factor expression or survival-associated phenotypes, such as stress tolerance, biofilm formation, or cell-cell communication, to illustrate how riboregulation factors into regulatory networks in bacterial pathogens.
Epidemiology of children with head injury: a national overview
Authors: Trefan L , Houston R , Pearson G , Edwards R , Hyde P , Maconochie I , Parslow RC , Kemp A
Published: May 2016

Background

The National Confidential Enquiry describes the epidemiology of children admitted to hospital with head injury.

Method

Children (<15 years old) who died or were admitted for >4 h with head injury were identified from 216 UK hospitals (1 September 2009 to 28 February 2010). Data were collected using standard proformas and entered on to a database. A descriptive analysis of the causal mechanisms, child demographics, neurological impairment, CT findings, and outcome at 72 h are provided.

Results

Details of 5700 children, median age 4 years (range 0–14.9 years), were analysed; 1093 (19.2%) were <1 year old, 3500 (61.4%) were boys. There was a significant association of head injury with social deprivation 39.7/100 000 (95% CI 37.0 to 42.6) in the least deprived first quintile vs. 55.1 (95% CI 52.1 to 58.2) in the most deprived fifth quintile (p<0.01). Twenty-four children died (0.4%). Most children were admitted for one night or less; 4522 (79%) had a Glasgow Coma Scale score of 15 or were Alert (on AVPU (Alert, Voice, Pain, Unresponsive)). The most common causes of head injury were falls (3537 (62.1%); children <5 years), sports-related incidents (783 (13.7%); median age 12.4 years), or motor vehicle accidents (MVAs) (401 (7.1%); primary-school-aged children). CT scans were performed in 1734 (30.4%) children; 536 (30.9%) were abnormal (skull fracture and/or intracranial injury or abnormality): 269 (7.6%) were falls, 82 (10.5%) sports related and 100 (25%). A total of 357 (6.2%) children were referred to social care because of child protection concerns (median age 9 months (range 0–14.9 years)).

Conclusions

The data described highlight priorities for targeted age-specific head injury prevention and have the potential to provide a baseline to evaluate the effects of regional trauma networks (2012) and National Institute of Health and Care Excellence (NICE) head injury guidelines (2014), which were revised after the study was completed.
Evaluation of delayed puberty: what diagnostic tests should be performed in the seemingly otherwise well adolescent?
Authors: Abitbol Leah , Zborovski Stephen , Palmert Mark R
Published: May 2016

Delayed puberty (DP) is defined as the lack of pubertal development by an age that is 2–2.5 SDs beyond the population mean. Although it generally represents a normal variant in pubertal timing, concern that DP could be the initial presentation of a serious underlying disorder has led to a diagnostic approach that is variable and may include tests that are unnecessary and costly. In this review, we examine available literature regarding the recommended diagnostic tests and aetiologies identified during the evaluation of youth with DP. We view this literature through the prism of the seemingly otherwise well adolescent. To provide further clinical context, we also evaluate the clinical and laboratory data from patients seen with DP in our centre over a 2-year period. The literature and our data reveal wide variability in the number of tests performed and raise the question of whether tests, other than gonadotropins, obtained in the absence of signs or symptoms of an underlying disorder are routinely warranted. Together this information provides a pragmatic rationale for revisiting recommendations calling for broad testing during the initial diagnostic evaluation of an otherwise healthy adolescent with DP. We highlight the need for further research comparing the utility of broader screening with a more streamlined approach, such as limiting initial testing to gonadotropins and a bone age, which, while not diagnostic, is often useful for height prediction, followed by close clinical monitoring. If future research supports a more streamlined approach to DP, then much unnecessary testing could be eliminated.
A cross-sectional study of hyponatraemia among elderly patients with heart failure in Uganda
Authors: Nankabirwa Harriet , Kalyesubula Robert , Ssinabulya Isaac , Katabira Elly T , Cumming Robert G
Published: May 2016

Background

Hyponatraemia is a common electrolyte disturbance among older patients. We determined the prevalence of and factors associated with hyponatraemia among older patients with predominantly acute decompensated heart failure attending a tertiary hospital in Kampala, Uganda.

Objectives

Main study aim: (1) to determine the prevalence of hyponatraemia among patients aged 60 years and above with heart failure attending Mulago National Referral Hospital; (2) to describe the factors associated with hyponatraemia among patients aged 60 years and above with heart failure attending Mulago National Referral Hospital.

Setting

The study was conducted in one tertiary hospital located in the northeast of Kampala, Uganda.

Participants

400 adults aged 60 years and above were identified for the study. Of these, 188 were excluded as they did not fulfil the inclusion criteria and one declined to participate, leaving a final study group of 211 older adults aged 60 years and above, with a clinical diagnosis of heart failure using Framingham's criteria.

Results

The prevalence of hyponatraemia was 24.2% (51/211). Hyponatraemia was mainly found in patients with mild-to-moderate heart failure, New York Heart Association classes 2 and 3. Of the 51 patients with hyponatraemia, 27 (52.9%) had mild hyponatraemia, while 24 (47.1%) had moderate to severe hyponatraemia of 130–125 mmol/L. History of vomiting (OR=2.94, 95% CI 1.29 to 6.70, p=0.010) and use of loop diuretics (OR=2.71, 95% CI 1.13 to 6.52, p=0.026) were identified as independent factors associated with hyponatraemia among older patients with heart failure.

Conclusions

Our study revealed a relatively high prevalence of hyponatraemia among older patients with mild to moderate heart failure. Patients presenting with a history of vomiting from any cause or use of loop diuretics were more likely to have hyponatraemia.
Telerehabilitation after total knee replacement in Italy: cost-effectiveness and cost-utility analysis of a mixed telerehabilitation-standard rehabilitation programme compared with usual care
Authors: Fusco Francesco , Turchetti Giuseppe
Published: May 2016

Objectives

To assess cost-effectiveness and cost utility of telerehabilitation (TR) versus standard rehabilitation (SR) after total knee replacement (TKR).

Design

Markov decision modelling of cost-effectiveness and cost-utility analysis based on patient-level and secondary data sources employing Italian National Health Service (NHS; Ita-NHS) and Society perspectives.

Setting

Primary care units (PCUs) in Italy.

Participants

Patients discharged after TKR.

Interventions

Mixed SR-TR service (10 face-to-face sessions and 10 telesessions) versus SR (20 face-to-face sessions)

Primary and secondary outcome measures

The incremental cost per additional knee flexion range of motion (ROM) and per QALY gained by SR-TR compared with SR. Second, we considered the probability of being cost-effective and the probability of being more effective and less expensive.

Results

TR appears to be the cost-effective in the base case and in all of the considered scenarios, but is no longer more effective and less expensive if transportation costs are excluded. Comparing SR-TR with SR, the incremental cost-effectiveness ratio (ICER) adopting the Ita-NHS perspective for the base case was −€117/ROM gained. The cost-effectiveness probability for SR-TR was 0.98 (ceiling ratio: €50/ROM), while the joint probability of being more effective and less expensive was 0.87. Assuming that TR would increase health-related quality of life (HRQOL) utilities by 2.5%, the ICER adopting Ita-NHS perspective is −€960/QALY (cost-effectiveness probability: 1; ceiling ratio: €30 000/QALY). All the performed sensitivity analyses did not change the conclusions, but if transportation costs were excluded, the probability for SR-TR of being more clinically effective and less expensive reduced to 0.56.

Conclusions

The analysis suggested SR-TR to be cost-effective, even less expensive and more effective if the PCUs provide ambulance transportations. However, the uncertainty related to TR costs, HRQOL and long-term clinical outcomes raises important topics for future research, which should be addressed to confirm our estimates.

Trial registration number

ISRCTN45837371.
Nationwide population-based cohort study of uterine rupture in Belgium: results from the Belgian Obstetric Surveillance System
Authors: Vandenberghe G , De Blaere M , Van Leeuw V , Roelens K , Englert Y , Hanssens M , Verstraelen H
Published: May 2016

Objectives

We aimed to assess the prevalence of uterine rupture in Belgium and to evaluate risk factors, management and outcomes for mother and child.

Design

Nationwide population-based prospective cohort study.

Setting

Emergency obstetric care. Participation of 97% of maternity units covering 98.6% of the deliveries in Belgium.

Participants

All women with uterine rupture in Belgium between January 2012 and December 2013. 8 women were excluded because data collection forms were not returned.

Results

Data on 90 cases of confirmed uterine rupture were obtained, of which 73 had a previous Caesarean section (CS), representing an estimated prevalence of 3.6 (95% CI 2.9 to 4.4) per 10 000 deliveries overall and of 27 (95% CI 21 to 33) and 0.7 (95% CI 0.4 to 1.2) per 10 000 deliveries in women with and without previous CS, respectively. Rupture occurred during trial of labour after caesarean section (TOLAC) in 57 women (81.4%, 95% CI 68% to 88%), with a high rate of augmented (38.5%) and induced (29.8%) labour. All patients who underwent induction of labour had an unfavourable cervix at start of induction (Bishop Score ≤7 in 100%). Other uterine surgery was reported in the history of 22 cases (24%, 95% CI 17% to 34%), including 1 case of myomectomy, 3 cases of salpingectomy and 2 cases of hysteroscopic resection of a uterine septum. 14 cases ruptured in the absence of labour (15.6%, 95% CI 9.5% to 24.7%). No mothers died; 8 required hysterectomy (8.9%, 95% CI 4.6% to 16.6%). There were 10 perinatal deaths (perinatal mortality rate 117/1000 births, 95% CI 60 to 203) and perinatal asphyxia was observed in 29 infants (34.5%, 95% CI 25.2% to 45.1%).

Conclusions

The prevalence of uterine rupture in Belgium is similar to that in other Western countries. There is scope for improvement through the implementation of nationally adopted guidelines on TOLAC, to prevent use of unsafe procedures, and thereby reduce avoidable morbidity and mortality.
Access to primary care for socioeconomically disadvantaged older people in rural areas: a realist review
Authors: Ford John A , Wong Geoff , Jones Andy P , Steel Nick
Published: May 2016

Objective

The aim of this review is to identify and understand the contexts that effect access to high-quality primary care for socioeconomically disadvantaged older people in rural areas.

Design

A realist review.

Data sources

MEDLINE and EMBASE electronic databases and grey literature (from inception to December 2014).

Eligibility criteria for selecting studies

Broad inclusion criteria were used to allow articles which were not specific, but might be relevant to the population of interest to be considered. Studies meeting the inclusion criteria were assessed for rigour and relevance and coded for concepts relating to context, mechanism or outcome.

Analysis

An overarching patient pathway was generated and used as the basis to explore contexts, causal mechanisms and outcomes.

Results

162 articles were included. Most were from the USA or the UK, cross-sectional in design and presented subgroup data by age, rurality or deprivation. From these studies, a patient pathway was generated which included 7 steps (problem identified, decision to seek help, actively seek help, obtain appointment, get to appointment, primary care interaction and outcome). Important contexts were stoicism, education status, expectations of ageing, financial resources, understanding the healthcare system, access to suitable transport, capacity within practice, the booking system and experience of healthcare. Prominent causal mechanisms were health literacy, perceived convenience, patient empowerment and responsiveness of the practice.

Conclusions

Socioeconomically disadvantaged older people in rural areas face personal, community and healthcare barriers that limit their access to primary care. Initiatives should be targeted at local contextual factors to help individuals recognise problems, feel welcome, navigate the healthcare system, book appointments easily, access appropriate transport and have sufficient time with professional staff to improve their experience of healthcare; all of which will require dedicated primary care resources.
Determinants of adherence to seasonal influenza vaccination among healthcare workers from an Italian region: results from a cross-sectional study
Authors: Durando P , Alicino C , Dini G , Barberis I , Bagnasco A M , Iudici R , Zanini M , Martini M , Toletone A , Paganino C , Massa E , Orsi A , Sasso L
Published: May 2016

Objectives

Notwithstanding decades of efforts to increase the uptake of seasonal influenza (flu) vaccination among European healthcare workers (HCWs), the immunisation rates are still unsatisfactory. In order to understand the reasons for the low adherence to flu vaccination, a study was carried out among HCWs of two healthcare organisations in Liguria, a region in northwest Italy.

Methods

A cross-sectional study based on anonymous self-administered web questionnaires was carried out between October 2013 and February 2014. Through univariate and multivariate regression analysis, the study investigated the association between demographic and professional characteristics, knowledge, beliefs and attitudes of the study participants and (i) the seasonal flu vaccination uptake in the 2013/2014 season and (ii) the self-reported number of flu vaccination uptakes in the six consecutive seasons from 2008/2009 to 2013/2014.

Results

A total of 830 HCWs completed the survey. Factors statistically associated with flu vaccination uptake in the 2013/2014 season were: being a medical doctor and agreeing with the statements ‘flu vaccine is safe’, ‘HCWs have a higher risk of getting flu’ and ‘HCWs should receive flu vaccination every year’. A barrier to vaccination was the belief that pharmaceutical companies influence decisions about vaccination strategies.

Discussion

All the above-mentioned factors, except the last one, were (significantly) associated with the number of flu vaccination uptakes self-reported by the respondents between season 2008/2009 and season 2013/2014. Other significantly associated factors appeared to be level of education, being affected by at least one chronic disease, and agreeing with mandatory flu vaccination in healthcare settings.

Conclusions

This survey allows us to better understand the determinants of adherence to vaccination as a fundamental preventive strategy against flu among Italian HCWs. These findings should be used to improve and customise any future promotion campaigns to overcome identified barriers to immunisation.
ADA2 deficiency: case report of a new phenotype and novel mutation in two sisters
Authors: Uettwiller F , Sarrabay G , Rodero M P , Rice G I , Lagrue E , Marot Y , Deiva K , Touitou I , Crow Y J , Quartier P
Published: May 2016

The objective of this paper is to

describe the phenotype compound heterozygote for mutations inCECR1in two children. We describe the clinical and immunological phenotype, including the assessment of ADA2 activity, cytokine expression, interferon-stimulated and neutrophil-stimulated gene signatures, and the results of CECR1 sequencing. The first patient presented with intermittent fever, cutaneous vasculitis, myalgia and muscle inflammation on MRI leading to a provisional diagnosis of periarteritis nodosa. Subsequently, two cerebral lacunar lesions were identified following a brain stroke. Clinical features improved on anti-tumour necrosis factor therapy. The first patient's sister demonstrated early-onset, long-lasting anaemia with mild biological inflammation; at the ages of 3 and 5 years, she had presented 2 acute, transient neurological events with lacunar lesions on MRI. CECR1 sequencing identified both sisters to be compound heterozygous for a p.Tyr453Cys mutation and a previously undescribed deletion of exon 7. ADA2 activity was reduced by 50%. Neutrophil-stimulated genes were not overexpressed, but interferon-stimulated genes were. The expression of a panel of other cytokine transcripts was not significantly altered. In conclusion, searching forCECR1mutation or assessing ADA2 activity should be considered in patients with an atypical presentation of inflammatory disease.
Nutritional management in a horse after caecocolic intussusception with a large partial typhlectomy
Authors: Wambacq Wendy An , Paulussen Ellen , Lefère Laurence , van Bergen Thomas , Hesta Myriam
Published: May 2016

A 17‐year‐old Haflinger gelding was referred to the Faculty of Veterinary Medicine for evaluation and treatment of acute colic. Clinical examination resulted in a presumptive diagnosis of caecocolic intussusception, which was confirmed by exploratory laparotomy. Due to severe compromise of the caecal apex wall, a partial typhlectomy was performed. Information about optimal nutritional management of typhlectomised horses is unfortunately rather limited. While immediate postsurgical support focuses on maintaining current bodyweight, the long‐term goal is to obtain and maintain an optimal body condition score. In this case, postoperative nutritional support focused initially on a low‐bulk diet that would be primarily digested in the small intestine, while providing sufficient fibre to provide colonocytes with an energy source. One month after surgery, a slow transition towards a more traditional diet containing long‐stem roughage was made. This is the first case report describing a detailed successful nutritional approach up until six months postoperatively.
Providing laying hens in group-housed enriched cages with access to barley silage reduces aggressive and feather-pecking behaviour
Authors: Johannson S.G. , Raginski C. , Schwean-Lardner K. , Classen H.L.
Published: May 2016

Two trials were conducted to study the effect of feeding barley silage on the behaviour and performance of beak-trimmed laying hens. In each trial, 20 hens and 2 roosters were housed in each of eight group-housed enriched cages, with four cages provided a laying hen diet and four cages additionally given free access to barley silage. Egg production and quality, and hen behaviour were assessed throughout the trials (Trial One 20–30 weeks; Trial Two 19–28 weeks). Data were analysed using Proc Mixed of SAS 9.4 and differences were significant when P ≤ 0.05. Silage-fed hens consumed 41 (13.9 g DM) and 50 (13.5 g DM) grams of silage per hen per day in Trials One and Two, respectively, while consuming less layer diet. Birds fed silage spent less time expressing aggressive and feather-pecking behaviours and in nest boxes, and more time feeding than control birds. Egg production, egg quality, and bird weight were not affected by treatment; yolk colour was darker for the silage treatment. Feathering quality was improved in silage-fed birds compared to control birds. It was concluded that providing hens with access to barley silage can improve welfare indicators without negatively affecting the egg production and egg quality.
Conflicts resolution based construction temporary facilities layout planning in large-scale construction projects
Authors: Song Xiaoling , Xu Jiuping , Shen Charles , Peña-Mora Feniosky
Published: May 2016

The construction temporary facilities layout planning (CTFLP) is important and complex in large-scale construction projects. During the CTFLP process, the general contractor has conflicts with many subcontractors regarding other pre-planning tasks, such as the transport routes planning (TRP). The resolution of the conflicts is crucial and this study attempts to determine the CTFLP considering conflicts resolution. Specifically, a novel methodology based on conflicts resolution between the CTFLP and the TRP is proposed, which can be adapted for future CTFLP based on resolving other types of conflicts. The two procedures-based methodology is composed of a bi-level model and a solution approach. The methodology is applied to a dam construction project in China to test the applicability, and the results show that it is able to obtain a satisfactory CTFLP and prevents some crucial conflicts, thus, promoting construction operations and making a significant contribution to the practice of construction projects.
Construction of Reconfigurable Clock Trees for MCMM Designs Using Mode Separation and Scenario Compression
Authors: Ewetz Rickard , Koh Cheng-Kok
Published: May 2016

The clock networks of many modern circuits have to operate in multiple corners and multiple modes (MCMM). We propose to construct mode-reconfigurable clock trees (MRCTs) based on mode separation and scenario compression. The technique of scenario compression is proposed to consider the timing constraints in multiple scenarios at the same time, compressing the MCMM problem into an equivalent single-corner multiple-mode (SCMM), or single-corner single-mode (SCSM) problem. The compression is performed by combining the skew constraints of the different scenarios in skew constraint graphs based on delay linearization and dominating skew constraints. An MRCT consists of several clock trees and mode separation involves, depending on the active mode, selecting one of the clock trees to deliver the clock signal. To limit the overhead, the bottom part (closer to the clock sinks) of all the different clock trees are shared and only the top part (closer to the clock source) of the clock network is mode reconfigurable. The reconfiguration is realized using OR-gates and a one-input-multiple-output demultiplexer. The experimental results show that for a set of synthesized MCMM circuits, with 715 to 13, 216 sequential elements, the proposed approach can achieve high yield.
Reconstruction of Personalized 3D Face Rigs from Monocular Video
Authors: Garrido Pablo , Zollhöfer Michael , Casas Dan , Valgaerts Levi , Varanasi Kiran , Pérez Patrick , Theobalt Christian
Published: May 2016

We present a novel approach for the automatic creation of a personalized high-quality 3D face rig of an actor from just monocular video data (e.g., vintage movies). Our rig is based on three distinct layers that allow us to model the actor’s facial shape as well as capture his person-specific expression characteristics at high fidelity, ranging from coarse-scale geometry to fine-scale static and transient detail on the scale of folds and wrinkles. At the heart of our approach is a parametric shape prior that encodes the plausible subspace of facial identity and expression variations. Based on this prior, a coarse-scale reconstruction is obtained by means of a novel variational fitting approach. We represent person-specific idiosyncrasies, which cannot be represented in the restricted shape and expression space, by learning a set of medium-scale corrective shapes. Fine-scale skin detail, such as wrinkles, are captured from video via shading-based refinement, and a generative detail formation model is learned. Both the medium- and fine-scale detail layers are coupled with the parametric prior by means of a novel sparse linear regression formulation. Once reconstructed, all layers of the face rig can be conveniently controlled by a low number of blendshape expression parameters, as widely used by animation artists. We show captured face rigs and their motions for several actors filmed in different monocular video formats, including legacy footage from YouTube, and demonstrate how they can be used for 3D animation and 2D video editing. Finally, we evaluate our approach qualitatively and quantitatively and compare to related state-of-the-art methods.
Correctness and Completeness of Logic Programs
Authors: Drabent Włodzimierz
Published: May 2016

We discuss proving correctness and completeness of definite clause logic programs. We propose a method for proving completeness, while for proving correctness we employ a method that should be well known but is often neglected. Also, we show how to prove completeness and correctness in the presence of SLD-tree pruning, and point out that approximate specifications simplify specifications and proofs.

We compare the proof methods to declarative diagnosis (algorithmic debugging), showing that approximate specifications eliminate a major drawback of the latter. We argue that our proof methods reflect natural declarative thinking about programs, and that they can be used, formally or informally, in everyday programming.
Narrow Proofs May Be Maximally Long
Authors: Atserias Albert , Lauria Massimo , Nordström Jakob
Published: May 2016

We prove that there are 3-CNF formulas over
n
variables that can be refuted in resolution in width
w
but require resolution proofs of size
n

Ω(
w
)

. This shows that the simple counting argument that any formula refutable in width
w
must have a proof in size
n

O(
w
)

is essentially tight. Moreover, our lower bound generalizes to polynomial calculus resolution and Sherali-Adams, implying that the corresponding size upper bounds in terms of degree and rank are tight as well. The lower bound does not extend all the way to Lasserre, however, since we show that there the formulas we study have proofs of constant rank and size polynomial in both
n
and
w
.
Library-Based Placement and Routing in FPGAs with Support of Partial Reconfiguration
Authors: Mao Fubing , Chen Yi-Chung , Zhang Wei , Li Hai (Helen) , He Bingsheng
Published: May 2016

While traditional Field-Programmable Gate Array design flow usually employs fine-grained tile-based placement, modular placement is increasingly required to speed up the large-scale placement and save the synthesis time. Moreover, the commonly used modules can be pre-synthesized and stored in the library for design reuse to significantly save the design, verification time, and development cost. Previous work mainly focuses on modular floorplanning without module placement information. In this article, we propose a library-based placement and routing flow that best utilizes the pre-placed and routed modules from the library to significantly save the execution time while achieving the minimal area-delay product. The flow supports the static and reconfigurable modules at the same time. The modular information is represented in the B*-Tree structure, and the B*-Tree operations are amended together with Simulated Annealing to enable a fast search of the placement space. Different width-height ratios of the modules are exploited to achieve area-delay product optimization. Partial reconfiguration-aware routing using pin-to-wire abutment is proposed to connect the modules after placement. Our placer can reduce the compilation time by 65% on average with 17% area and 8.2% delay overhead compared with the fine-grained results of Versatile Place and Route through the reuse of module information in the library for the base architecture. For other architectures, the area increase ranges from 8.32% to 25.79%, the delay varies from − 13.66% to 19.79%, and the runtime improves by 43.31% to 77.2%.
Emerging role for regulated in development and DNA damage 1 (REDD1) in the regulation of skeletal muscle metabolism
Authors: Gordon Bradley S. , Steiner Jennifer L. , Williamson David L. , Lang Charles H. , Kimball Scot R.
Published: May 2016

Since its discovery, the protein regulated in development and DNA damage 1 (REDD1) has been implicated in the cellular response to various stressors. Most notably, its role as a repressor of signaling through the central metabolic regulator, the mechanistic target of rapamycin in complex 1 (mTORC1) has gained considerable attention. Not surprisingly, changes in REDD1 mRNA and protein have been observed in skeletal muscle under various physiological conditions (e.g., nutrient consumption and resistance exercise) and pathological conditions (e.g., sepsis, alcoholism, diabetes, obesity) suggesting a role for REDD1 in regulating mTORC1-dependent skeletal muscle protein metabolism. Our understanding of the causative role of REDD1 in skeletal muscle metabolism is increasing mostly due to the availability of genetically modified mice in which the REDD1 gene is disrupted. Results from such studies provide support for an important role for REDD1 in the regulation of mTORC1 as well as reveal unexplored functions of this protein in relation to other aspects of skeletal muscle metabolism. The goal of this work is to provide a comprehensive review of the role of REDD1 (and its paralog REDD2) in skeletal muscle during both physiological and pathological conditions.
Selective and inducible targeting of CD11b+mononuclear phagocytes in the murine lung with hCD68-rtTA transgenic systems
Authors: McCubbrey Alexandra L. , Barthel Lea , Mould Kara J. , Mohning Michael P. , Redente Elizabeth F. , Janssen William J.
Published: May 2016

During homeostasis two distinct macrophage (Mø) populations inhabit the lungs: tissue Mø (often called interstitial Mø) and resident alveolar Mø (resAMø). During acute lung inflammation, monocytes from the circulation migrate to areas of injury where they mature into a third Mø population: recruited Mø. Resident AMø uniquely express low levels of CD11b and high levels of CD11c. In comparison, recruited Mø and tissue Mø express high levels of CD11b and low levels of CD11c. It is likely that these three Mø subpopulations play distinct roles in injury and disease states; however, tools with which to individually target or track these populations are lacking. Here we demonstrate the utility of an hCD68-rtTA transgenic system for specific, robust, and inducible targeting of CD11b+recruited Mø and tissue Mø in the murine lung with negligible activation in resAMø. Using hCD68rtTA-GFP reporter mice, we show both during homeostasis and inflammation that administration of doxycycline induces tet-On reporter expression in recruited Mø and tissue Mø but not in resident AMø. We further demonstrate how hCD68-rtTA can be effectively combined with tet-On Cre to target these same recMø and tissue Mø. Accordingly, the hCD68-rtTA system is a powerful new tool that can be used for lineage tracing, fate mapping, and gene deletion in a variety of murine models, thereby enabling sophisticated investigation of the unique role of these CD11b+Mø during lung heath and disease.
Early coagulation events induce acute lung injury in a rat model of blunt traumatic brain injury
Authors: Yasui Hideki , Donahue Deborah L. , Walsh Mark , Castellino Francis J. , Ploplis Victoria A.
Published: May 2016

Acute lung injury (ALI) and systemic coagulopathy are serious complications of traumatic brain injury (TBI) that frequently lead to poor clinical outcomes. Although the release of tissue factor (TF), a potent initiator of the extrinsic pathway of coagulation, from the injured brain is thought to play a key role in coagulopathy after TBI, its function in ALI following TBI remains unclear. In this study, we investigated whether the systemic appearance of TF correlated with the ensuing coagulopathy that follows TBI in ALI using an anesthetized rat blunt trauma TBI model. Blood and lung samples were obtained after TBI. Compared with controls, pulmonary edema and increased pulmonary permeability were observed as early as 5 min after TBI without evidence of norepinephrine involvement. Systemic TF increased at 5 min and then diminished 60 min after TBI. Lung injury and alveolar hemorrhaging were also observed as early as 5 min after TBI. A biphasic elevation of TF was observed in the lungs after TBI, and TF-positive microparticles (MPs) were detected in the alveolar spaces. Fibrin(ogen) deposition was also observed in the lungs within 60 min after TBI. Additionally, preadministration of a direct thrombin inhibitor, Refludan, attenuated lung injuries, thus implicating thrombin as a direct participant in ALI after TBI. The results from this study demonstrated that enhanced systemic TF may be an initiator of coagulation activation that contributes to ALI after TBI.
Acute Deep Vein Thrombosis in Venous Aneurysm following Closure of the Chronic Traumatic Arteriovenous Fistulae of the Lower Extremities
Authors: Orrapin Saranat , Arworn Supapong , Rerkasem Kittipan
Published: May 2016

Chronic traumatic arteriovenous fistula (AVF) commonly results from an unrecognized vascular injury. In this report, there were two cases of chronic traumatic AVF of the legs with a long history of stab (case 1) and shotgun wounds (case 2). Both cases presented with varicose veins together with hyperpigmentation around the ankle of the affected leg. Angiograms showed a single large AVF in case 1, whereas, in case 2, there was a single large AVF together with multiple small AVFs. In both cases large venous aneurysm was found next to a large AVF. An open surgical AVF closure for the large AVF was performed in case 1 successfully, but patient developed acute deep vein thrombosis (DVT) in a large venous aneurysm. In the second case, in order to prevent DVT, only closure of the large AVF was performed, which preserved arterial flow into the venous aneurysm. Case 2 did not have acute DVT. This report raised the concern about acute DVTs in venous aneurysms following the closure of chronic traumatic AVF in terms of prevention. Also chronic traumatic AVF is commonly due to misdiagnosis in the initial treatment, so complete and serial physical examinations in penetrating vascular injury patients are of paramount importance.