bluuebunny/crossref_metadata_2025 huggingface dataset:
Flood Susceptibility Assessment through Statistical Models and HEC-RAS Analysis for Sustainable Management in Essaouira Province, Morocco
Authors: Khouz Abdellah , Trindade Jorge , Santos Pedro Pinto , Oliveira Sérgio C. , El Bchari Fatima , Bougadir Blaid , Garcia Ricardo A. C. , Reis Eusébio , Jadoud Mourad , Saouabe Tarik , Rachidi Said
Published: December 2023

Floods are natural disasters that often impact communities living in low-lying areas in the northern and central parts of Morocco. In this study, our aim was to create a flood susceptibility map using three methods; the hierarchy process (AHP) frequency ratio model (FR) and the weights of evidence (WoE) model. We extensively examined the area identified by these approaches using a hydraulic analysis software called HEC-RAS (version 6.3.1). Our analysis focused on the Essaouira watersheds in Morocco, where we identified around 197 flood locations. Out of these, we randomly selected 70% for modeling purposes while the remaining 30% were used for validation. Ten factors that influence floods were considered, such as slope, elevation, proximity to rivers, drainage density, stream order, land use patterns, rainfall data, lithology (permeability level) index (TWI), and curvature. We obtained these factors from data sources. Finally, we generated a flood susceptibility map and evaluated its accuracy by calculating the area under the curve (AUC). The validation results confirmed that all three models were robust and effective with an AUC of 90. Moreover, the research uncovered a trend of vulnerability with the most susceptible area being in close proximity to the city of Essaouira along the Oued Ksob. A detailed analysis using HEC-RAS was conducted at this identified location, pinpointing the village of Diabat as highly exposed. These findings hold significance for flood management, empowering decision makers, scholars, and urban planners to make informed choices and implement strategies that can minimize the impact of floods in susceptible regions while minimizing potential damages.
Empirical Suitability of Scoring Systems of Observational Techniques for Repetitive Movements Based on Discomfort
Authors: Kee Dohyung
Published: December 2023

This study empirically investigated the effects of repetitive movements of body parts through an experiment, and evaluated the suitability of the scoring systems of the existing observational methods for repetitive movements, based on the experimental results. Eighteen healthy college students participated in the experiment to assess discomfort, wherein joint movement, its repetition, and external load were used as independent variables. Postural loads for 16 postures used in the experiment were assessed using rapid upper limb assessment (RULA) and loading on the entire body assessment (LEBA). Three independent variables, joint motion, its repetition, and external load, as well as the interaction between motion repetition and external load, had significant effects on discomfort. Joint motion and external load significantly affected the RULA grand score, whereas all three independent variables affected the LEBA score. This finding may indicate that LEBA more accurately reflects the effect of repetitive body part movements. Additionally, the scoring systems for repetitive back motions by quick exposure check (QEC) and for repetitive wrist motions via a risk assessment and management tool for manual handling proactively II (RAMP II) may be reasonable based on the results of this study. The findings of this study can be used as reference information for better evaluation of postural loads assessed using the existing observational techniques, and as useful baseline data for the development of a new observational method to accurately assess stress caused by repetitive movements.
Novel Telehealth Adaptations for Evidence-Based Outpatient Suicide Treatment: Feasibility and Effectiveness of the Crisis Care Program
Authors: O’Neill J. Conor , O’Callaghan Erin T. , Sullivan Scott , Winsberg Mirène
Published: December 2023

Background: Suicide rates in the United States have escalated dramatically over the past 20 years and remain a leading cause of death. Access to evidenced-based care is limited, and telehealth is well-positioned to offer novel care solutions. The Crisis Care program is a suicide-specific treatment program delivered within a national outpatient telehealth setting using a digitally adapted version of the Collaborative Assessment and Management of Suicidality (CAMS) as the framework of care. This study investigates the feasibility and preliminary effectiveness of Crisis Care as scalable suicide-specific treatment model. Methods: Patient engagement, symptom reduction, and care outcomes were examined among a cohort of patients (n = 130) over 16 weeks. The feasibility of implementation was assessed through patient engagement. Clinical outcomes were measured with PHQ-9, GAD-7, and the CAMS SSF-4 rating scales. Results: Over 85% of enrolled patients were approved for Crisis Care at intake, and 83% went on to complete at least four sessions (the minimum required to graduate). All patient subgroups experienced declines in depressive symptoms, anxiety symptoms, suicidal ideation frequency, and suicide-specific risk factors. Conclusions: Results support the feasibility and preliminary effectiveness of Crisis Care as a suicide-specific care solution that can be delivered within a stepped-care model in an outpatient telehealth setting.
Impact of Exercise Interventions on Sustained Brain Health Outcomes in Frail Older Individuals: A Comprehensive Review of Systematic Reviews
Authors: Furtado Guilherme Eustáquio , Reis Anne Sulivan Lopes da Silva , Braga-Pereira Ricardo , Caldo-Silva Adriana , Teques Pedro , Sampaio António Rodrigues , Santos Carlos André Freitas dos , Bachi André Luís Lacerda , Campos Francisco , Borges Grasiely Faccin , Brito-Costa Sónia
Published: December 2023

Several systematic review studies highlight exercise’s positive impact on brain health outcomes for frail individuals. This study adopts a Comprehensive Review of reviews (CRs) approach to amalgamate data from existing reviews, focusing on exercise’s influence on brain health outcomes in older frail and pre-frail adults. The methodology involves a thorough search of Portuguese, Spanish, and English-indexed databases (i.e., Ebsco Health, Scielo, ERIC, LILACS, Medline, Web of Science, SportDiscus) from 1990 to 2022, with the AMSTAR-2 tool assessing evidence robustness. The search terms “physical exercise”, “elderly frail”, and “systematic review” were employed. Results: Out of 12 systematically reviewed studies, four presented high-quality (with metanalyses), while eight exhibit critically low quality. Positive trends emerge in specific cognitive and neuromotor aspects, yet challenges persist in psychosocial domains, complex cognitive tasks, and ADL outcomes. This study yields reasonable and promising evidence regarding exercise’s influence on quality of life and depression in frail older individuals. However, the impact on biochemical markers remains inconclusive, emphasizing the need for standardized methodologies. Conclusions: The findings highlight the importance of acknowledging methodological nuances for clinicians and policymakers when translating these results into impactful interventions for aging populations. This emphasizes the necessity for a comprehensive and customized approach to exercise interventions aimed at fostering the sustainability of overall well-being in older individuals, aligning with United Nations Sustainable Development Goal 3.
Developing an Instrument to Evaluate the Quality of Dementia Websites
Authors: Zhu Yunshu , Song Ting , Zhang Zhenyu , Yu Ping
Published: December 2023

In today’s digital era, health information, especially for conditions like dementia, is crucial. This study aims to develop an instrument, demenTia wEbsite measSurement insTrument (TEST), through four steps: identifying existing instruments, determining criteria, selecting and revising measurement statements, and validating the instrument from March to August 2020. Five health informatics experts used the content validity ratio (CVR) test for validation. Thirteen evaluators compared Fleiss Kappa and intraclass correlation coefficient (ICC) values across four dementia websites using TEST and another tool, DISCERN. TEST consists of seven criteria and 25 measurement statements focusing on content quality (relevance, credibility, currency) and user experience (accessibility, interactivity, attractiveness, privacy). CVR = 1 confirmed all statements as essential. The TEST demonstrated stronger consistency and assessor agreement compared to DISCERN, measured by Fleiss Kappa and ICC. Overall, it is a robust tool for reliable and user-friendly dementia resources, ensuring health holistic information accessibility.
The Evolution of the Concept of Underwater Cultural Heritage in Europe: A Review of International Law, Policy, and Practice
Authors: Calantropio Alessio , Chiabrando Filiberto
Published: December 2023

Underwater cultural heritage (UCH) is a diverse and valuable resource that includes shipwrecks, sunken cities, and other submerged archaeological sites. It is an important part of human history and culture and can significantly benefit society. However, various factors often neglect and threaten UCH, including climate change, pollution, and human activities. Several factors, including technological advances, the development of international law, and the growing awareness of the importance of cultural heritage, have influenced the evolution of the concept of UCH. In the early days of underwater archaeology, the focus was on recovering artifacts and treasures from shipwrecks. However, over time, there has been a shift towards a more holistic approach to the management of UCH, which emphasizes the importance of in situ preservation and the involvement of local communities. This review provides a chronological analysis of the evolution of the concept of UCH over the past 70 years and examines the main international conventions and charters developed to protect UCH. The review also discusses the relationship between UCH and marine protected areas (MPAs), the marine environment, and the coastal landscape.
Investigating Graduate Students’ Experiences through Structural Equation Modeling (SEM)
Authors: Collier Karen , Blanchard Margaret
Published: December 2023

This study used structural equation modeling (SEM) to investigate the role of graduate students’ experiences of microaffirmations, microaggressions, financial concerns, and mentor support (exogenous variables) on their perceptions of imposter syndrome, sense of belonging, and access and opportunities (endogenous variables). These success factors were measured using the Graduate Student Support Survey (GSSS), a 7-factor, 28-item survey. The GSSS success factors were mapped onto the basic needs components of self-determination theory: relatedness, competence, and autonomy. The SEM investigated the experiences of students based on a wide range of personal factors: enrollment status (full/part-time), gender, major (STEM/non-STEM), demographic group (race/ethnicity), degree type (M.S./Ph.D.), and family college experience (first/continuing generation). The SEM identified several significant pathways between the latent constructs (e.g., mentor support and sense of belonging) and differences based on demographic characteristics (e.g., STEM, female, and part-time student). Recognizing the impact of these factors on students’ well-being can spur relevant university administrators, faculty, and staff to take steps that will create a more inclusive campus climate that better supports graduate student success.
Evaluation of Nitrogen and Water Management Strategies to Optimize Yield in Open Field Cucumber (Cucumis sativus L.) Production
Authors: Bello Adewale Suraj , Huda Samsul , Chen Zhong-Hua , Khalid Muhammad Fasih , Alsafran Mohammed , Ahmed Talaat
Published: December 2023

Countries in arid climates, such as Qatar, require efficient water-saving strategies and nitrogen treatment for vegetable production. Vegetable importation constituted approximately USD 352 million of Qatar’s 2019 GDP; hence, enhancing local production is essential. This study investigated the effect of varying nitrogen and water levels on cucumber (Cucumis sativus L.) fruit yield. Various water management strategies were also evaluated. A split plot design was employed with two water levels (W1: 50% deficit irrigation, W2: 100% full irrigation) and three nitrogen levels (N1: 50 kg N ha−1, N2: 70 kg N ha−1, N3: 100 kg N ha−1) to examine cucumber yield and physiological response. Our findings revealed that using minimal drip irrigation and reducing nitrogen levels significantly enhanced the growth, SPAD index, fruit characteristics, and yield components of cucumber. Drip irrigation had a greater influence on cucumber production than nitrogen levels. Shoot height increased by 4% from W2N1 (T1) to W1N3 (T6) and 4.93% from W2N2 (T2) to W1N2 (T5). Fruit length and width increased by 10.63% and 13.41% from T2 and T1 to T5, respectively. The highest total yield occurred at T5, followed by T6, T2, W2N3 (T3), W1N1 (T4), and T1 at 34.5, 29.1, 27.6, 25.8, 25.2, and 20.4 t/ha, respectively. The optimal combination comprised 50% deficit irrigation (W1) and 70 kg N ha−1 (N2) nitrogen. These results suggest the importance of optimizing drip irrigation for achieving maximum cucumber fruit yield in arid climates.
Ecotoxicology Evaluation of a Fenton—Type Process Catalyzed with Lamellar Structures Impregnated with Fe or Cu for the Removal of Amoxicillin and Glyphosate
Authors: Lugo Lorena , Venegas Camilo , Guarin Trujillo Elizabeth , Diaz Granados-Ramírez Maria Alejandra , Martin Alison , Vesga Fidson-Juarismy , Pérez-Flórez Alejandro , Celis Crispín
Published: December 2023

Antibiotics and pesticides, as well as various emerging contaminants that are present in surface waters, raise significant environmental concerns. Advanced oxidation processes, which are employed to eliminate these substances, have demonstrated remarkable effectiveness. However, during the degradation process, by-products that are not completely mineralized are generated, posing a substantial risk to aquatic ecosystem organisms; therefore, it is crucial to assess effluent ecotoxicity following treatment. This study aimed to assess the toxicity of effluents produced during the removal of amoxicillin and glyphosate with a Fenton-type process using a laminar structure catalyzed with iron (Fe) and copper (Cu). The evaluation included the use of Daphnia magna, Selenastrum capricornutum, and Lactuca sativa, and mutagenicity testing was performed using strains TA98 and TA100 of Salmonella typhimurium. Both treated and untreated effluents exhibited inhibitory effects on root growth in L. sativa, even at low concentrations ranging from 1% to 10% v/v. Similarly, negative impacts on the growth of algal cells of S. capricornutum were observed at concentrations as low as 0.025% v/v, particularly in cases involving amoxicillin–copper (Cu) and glyphosate with copper (Cu) and iron (Fe). Notably, in the case of D. magna, mortality was noticeable even at concentrations of 10% v/v. Additionally, the treatment of amoxicillin with double-layer hydroxides of Fe and Cu resulted in mutagenicity (IM ≥ 2.0), highlighting the necessity to treat the effluent further from the advanced oxidation process to reduce ecological risks.
Geo-Referencing and Analysis of Entities Extracted from Old Drawings and Photos Using Computer Vision and Deep Learning Algorithms
Authors: David Liat , Zohar Motti , Shimshoni Ilan
Published: December 2023

This study offers a quantitative solution that automates the creation of a historical timeline starting with old drawings from the beginning of the 18th century and ending with present-day photographs of the Old City of Jerusalem. This is performed using GIScience approaches, computer vision, and deep learning. The motivation to select the Old City of Jerusalem is the substantial availability of old archival drawings and photographs, owing to the area’s significance throughout the years. This task is challenging, as drawings, old photographs, and new photographs exhibit distinct characteristics. Our method encompasses several key components for the analyses: a 2D location recommendation engine, which detects an approximate location in the image of 3D landmarks; 2D landmarks to 3D conversion; and 2D polygonal areas to 3D GIS polylines conversion. This is applied to the segmentation of built areas. To achieve more accurate results, Meta’s Segment Anything model was utilized, which eliminates the need for extensive data preparation, training, and validation, thus optimizing the process. Using such techniques enabled us to examine the landscape development throughout the last three centuries and gain deeper insights concerning the evolution of prominent landmarks and features such as built area over time.
Construction of a Real-Time Ship Trajectory Prediction Model Based on Ship Automatic Identification System Data
Authors: Xi Daping , Feng Yuhao , Jiang Wenping , Yang Nai , Hu Xini , Wang Chuyuan
Published: December 2023

The extraction of ship behavior patterns from Automatic Identification System (AIS) data and the subsequent prediction of travel routes play crucial roles in mitigating the risk of ship accidents. This study focuses on the Wuhan section of the dendritic river system in the middle reaches of the Yangtze River and the partial reticulated river system in the northern part of the Zhejiang Province as its primary investigation areas. Considering the structure and attributes of AIS data, we introduce a novel algorithm known as the Combination of DBSCAN and DTW (CDDTW) to identify regional navigation characteristics of ships. Subsequently, we develop a real-time ship trajectory prediction model (RSTPM) to facilitate real-time ship trajectory predictions. Experimental tests on two distinct types of river sections are conducted to assess the model’s reliability. The results indicate that the RSTPM exhibits superior prediction accuracy when compared to conventional trajectory prediction models, achieving an approximate 20 m prediction accuracy for ship trajectories on inland waterways. This showcases the advancements made by this model.
Circadian Regulation of Apolipoproteins in the Brain: Implications in Lipid Metabolism and Disease
Authors: Lee Chaeeun Hannah , Murrell Charlotte Ellzabeth , Chu Alexander , Pan Xiaoyue
Published: December 2023

The circadian rhythm is a 24 h internal clock within the body that regulates various factors, including sleep, body temperature, and hormone secretion. Circadian rhythm disruption is an important risk factor for many diseases including neurodegenerative illnesses. The central and peripheral oscillators’ circadian clock network controls the circadian rhythm in mammals. The clock genes govern the central clock in the suprachiasmatic nucleus (SCN) of the brain. One function of the circadian clock is regulating lipid metabolism. However, investigations of the circadian regulation of lipid metabolism-associated apolipoprotein genes in the brain are lacking. This review summarizes the rhythmic expression of clock genes and lipid metabolism-associated apolipoprotein genes within the SCN in Mus musculus. Nine of the twenty apolipoprotein genes identified from searching the published database (SCNseq and CircaDB) are highly expressed in the SCN. Most apolipoprotein genes (ApoE, ApoC1, apoA1, ApoH, ApoM, and Cln) show rhythmic expression in the brain in mice and thus might be regulated by the master clock. Therefore, this review summarizes studies on lipid-associated apolipoprotein genes in the SCN and other brain locations, to understand how apolipoproteins associated with perturbed cerebral lipid metabolism cause multiple brain diseases and disorders. This review describes recent advancements in research, explores current questions, and identifies directions for future research.
The Interactive Complex between Cytomegalovirus Kinase vCDK/pUL97 and Host Factors CDK7–Cyclin H Determines Individual Patterns of Transcription in Infected Cells
Authors: Schütz Martin , Cordsmeier Arne , Wangen Christina , Horn Anselm H. C. , Wyler Emanuel , Ensser Armin , Sticht Heinrich , Marschall Manfred
Published: December 2023

The infection of human cytomegalovirus (HCMV) is strongly determined by the host–cell interaction in a way that the efficiency of HCMV lytic replication is dependent on the regulatory interplay between viral and cellular proteins. In particular, the activities of protein kinases, such as cyclin-dependent kinases (CDKs) and the viral CDK ortholog (vCDK/pUL97), play an important role in both viral reproduction and virus–host interaction. Very recently, we reported on the complexes formed between vCDK/pUL97, human cyclin H, and CDK7. Major hallmarks of this interplay are the interaction between cyclin H and vCDK/pUL97, which is consistently detectable across various conditions and host cell types of infection, the decrease or increase in pUL97 kinase activity resulting from cyclin H knock-down or elevated levels, respectively, and significant trans-stimulation of human CDK7 activity by pUL97 in vitro. Due to the fact that even a ternary complex of vCDK/pUL97–cyclin H–CDK7 can be detected by coimmunoprecipitation and visualized by bioinformatic structural modeling, we postulated a putative impact of the respective kinase activities on the patterns of transcription in HCMV-infected cells. Here, we undertook a first vCDK/pUL97-specific transcriptomic analysis, which combined conditions of fully lytic HCMV replication with those under specific vCDK/pUL97 or CDK7 drug-mediated inhibition or transient cyclin H knockout. The novel results were further strengthened using bioinformatic modeling of the involved multi-protein complexes. Our data underline the importance of these kinase activities for the C-terminal domain (CTD) phosphorylation-driven activation of host RNA polymerase in HCMV-infected cells. The impact of the individual experimental conditions on differentially expressed gene profiles is described in detail and discussed.
Integrated Stress Response (ISR) Pathway: Unraveling Its Role in Cellular Senescence
Authors: Kalinin Alexander , Zubkova Ekaterina , Menshikov Mikhail
Published: December 2023

Cellular senescence is a complex process characterized by irreversible cell cycle arrest. Senescent cells accumulate with age, promoting disease development, yet the absence of specific markers hampers the development of selective anti-senescence drugs. The integrated stress response (ISR), an evolutionarily highly conserved signaling network activated in response to stress, globally downregulates protein translation while initiating the translation of specific protein sets including transcription factors. We propose that ISR signaling plays a central role in controlling senescence, given that senescence is considered a form of cellular stress. Exploring the intricate relationship between the ISR pathway and cellular senescence, we emphasize its potential as a regulatory mechanism in senescence and cellular metabolism. The ISR emerges as a master regulator of cellular metabolism during stress, activating autophagy and the mitochondrial unfolded protein response, crucial for maintaining mitochondrial quality and efficiency. Our review comprehensively examines ISR molecular mechanisms, focusing on ATF4-interacting partners, ISR modulators, and their impact on senescence-related conditions. By shedding light on the intricate relationship between ISR and cellular senescence, we aim to inspire future research directions and advance the development of targeted anti-senescence therapies based on ISR modulation.
Exploring the Effect of Deep-Sea Water on the Therapeutic Potential of the Anti-Inflammatory Response in an Indomethacin-Induced Gastric Ulcer Rat Model
Authors: Park Soo-yeon , Im Jin A , Kim Ji Yeon
Published: December 2023

Gastric ulcers are often exacerbated by factors such as nonsteroidal anti-inflammatory drugs (NSAIDs) and inflammation, and they have a substantial impact on a significant portion of the population. Notably, indomethacin is recognized as a prominent contributor to ulcers. This study investigated this potential method, with normalization to the anti-inflammatory and antiulcer properties of deep-sea water (DSW)-derived mineral water, using an indomethacin-induced gastric ulcer model in rats. The study involved four groups (n = 6 rats/group): normal control group (CON), indomethacin-only group (IND), indomethacin with trace mineral water group (TM), and indomethacin with high magnesium low sodium water group (HMLS). For three weeks, the CON and IND groups consumed tap water, while the TM and HMLS groups had access to mineral water. Gastric ulcers were induced on the final day using indomethacin, for all groups except the CON group. The results demonstrated that HMLS intake significantly improved gastric mucosal damage, preserved mucin stability, and increased gastric thickness, indicating its potential to prevent and alleviate indomethacin-induced gastric ulcers. Furthermore, HMLS consumption led to the upregulation of key genes associated with inflammation and a reduction in inflammatory cytokines. These findings suggest that DSW-derived mineral water, and particularly its high Mg2+ content, may offer promising health benefits including anti-inflammatory and anti-ulcer properties.
The Role of Short-Chain Fatty Acids and Altered Microbiota Composition in Autism Spectrum Disorder: A Comprehensive Literature Review
Authors: Lagod Piotr P. , Naser Saleh A.
Published: December 2023

Autism spectrum disorder (ASD) is a complex neurodevelopmental condition characterized by deficits in communication and social interactions, restrictive and repetitive behavior, and a wide range of cognitive impediments. The prevalence of ASD tripled in the last 20 years and now affects 1 in 44 children. Although ASD’s etiology is not yet elucidated, a growing body of evidence shows that it stems from a complex interplay of genetic and environmental factors. In recent years, there has been increased focus on the role of gut microbiota and their metabolites, as studies show that ASD patients show a significant shift in their gut composition, characterized by an increase in specific bacteria and elevated levels of short-chain fatty acids (SCFAs), especially propionic acid (PPA). This review aims to provide an overview of the role of microbiota and SCFAs in the human body, as well as possible implications of microbiota shift. Also, it highlights current studies aiming to compare the composition of the gut microbiome of ASD-afflicted patients with neurotypical control. Finally, it highlights studies with rodents where ASD-like symptoms or molecular hallmarks of ASD are evoked, via the grafting of microbes obtained from ASD subjects or direct exposure to PPA.
Control of CCR5 Cell-Surface Targeting by the PRAF2 Gatekeeper
Authors: Da Silva Elisa , Scott Mark G. H. , Enslen Hervé , Marullo Stefano
Published: December 2023

The cell-surface targeting of neo-synthesized G protein-coupled receptors (GPCRs) involves the recruitment of receptors into COPII vesicles budding at endoplasmic reticulum exit sites (ERESs). This process is regulated for some GPCRs by escort proteins, which facilitate their export, or by gatekeepers that retain the receptors in the ER. PRAF2, an ER-resident four trans- membrane domain protein with cytoplasmic extremities, operates as a gatekeeper for the GB1 protomer of the heterodimeric GABAB receptor, interacting with a tandem di-leucine/RXR retention motif in the carboxyterminal tail of GB1. PRAF2 was also reported to interact in a two-hybrid screen with a peptide corresponding to the carboxyterminal tail of the chemokine receptor CCR5 despite the absence of RXR motifs in its sequence. Using a bioluminescence resonance energy transfer (BRET)-based subcellular localization system, we found that PRAF2 inhibits, in a concentration-dependent manner, the plasma membrane export of CCR5. BRET-based proximity assays and Co-IP experiments demonstrated that PRAF2/CCR5 interaction does not require the presence of a receptor carboxyterminal tail and involves instead the transmembrane domains of both proteins. The mutation of the potential di-leucine/RXR motif contained in the third intracellular loop of CCR5 does not affect PRAF2-mediated retention. It instead impairs the cell-surface export of CCR5 by inhibiting CCR5’s interaction with its private escort protein, CD4. PRAF2 and CD4 thus display opposite roles on the cell-surface export of CCR5, with PRAF2 inhibiting and CD4 promoting this process, likely operating at the level of CCR5 recruitment into COPII vesicles, which leave the ER.
Antarctic Krill Oil from Euphausia superba Ameliorates Carrageenan-Induced Thrombosis in a Mouse Model
Authors: Lee Gi Ho , Lee Seung Yeon , Chae Ju Yeon , Kim Jae Won , Kim Jin-Hee , Jeong Hye Gwang
Published: December 2023

FJH-KO obtained from Antarctic krill, especially Euphausia superba, has been reported to contain high amounts of omega-3 polyunsaturated fatty acids (n-3 PUFA) and to exhibit anticancer and anti-inflammatory properties. However, its antithrombotic effects have not yet been reported. This study aimed to investigate the antithrombotic effects of FJH-KO in carrageenan-induced thrombosis mouse models and human endothelial cells. Thrombosis was induced by carrageenan injection, whereas the mice received FJH-KO pretreatment. FJH-KO attenuated carrageenan-induced thrombus formation in mouse tissue vessels and prolonged tail bleeding. The inhibitory effect of FJH-KO was associated with decreased plasma levels of thromboxane B2, P-selectin, endothelin-1, β-thromboglobulin, platelet factor 4, serotonin, TNF-α, IL-1β, and IL-6. Meanwhile, FJH-KO induced plasma levels of prostacyclin I2 and plasminogen. In vitro, FJH-KO decreased the adhesion of THP-1 monocytes to human endothelial cells stimulated by TNF-α via eNOS activation and NO production. Furthermore, FJH-KO inhibited the expression of TNF-α-induced adhesion molecules such as ICAM-1 and VCAM-1 by suppressing the NF-κB signaling pathway. Taken together, our study demonstrates that FJH-KO protects against carrageenan-induced thrombosis by regulating endothelial cell activation and has potential as an antithrombotic agent.
Preparation and Antimicrobial Activity of a Film-Forming Polyhexamethylene Biguanide Teat Disinfectant
Authors: Lu Yixing , Wang Di , Zhang Yongxiang , Hu Yueying , Lu Jiaxuan , Zeng Zhenling , Zeng Dongping
Published: December 2023

Bovine mastitis caused by infectious pathogens can lead to a decline in production performance and an increase in elimination rate, resulting in huge losses to the dairy industry. This study aims to prepare a novel dairy cow teat disinfectant with polyhexamethylene biguanide (PHMB) as the main bactericidal component and to evaluate its bactericidal activity in vitro and its disinfection effect in dairy cow teats. PHMB disinfectant with a concentration of 3 g/L was prepared with PVA-1788, propylene glycol and glycerol as excipients. When the dilution ratio is 1:4800 and the action time is 5 min, the PHMB teat disinfectant can reduce the four types of bacteria (S. agalactiae ATCC 12386, S. dysgalactiae ATCC 35666, S. aureus ATCC 6538, and E. coli ATCC 8099) by 99.99%. PHMB teat disinfectant applied on the skin of rabbits with four bacteria types achieved an average log10 reduction greater than 4. After 30 s of PHMB teat disinfectant dipping, the bacteria of cow teats were counted prior to disinfection. The mean log10 reduction in bacteria on the skin surface of 12 cows ranged from 0.99 to 3.52 after applying the PHMB teat disinfectant for 10 min. After 12 h, the PHMB teat disinfectant achieved an average log10 reduction in bacteria from 0.27 to 0.68 (compared with that prior to disinfection). These results suggested that PHMB teat disinfection has the potential to prevent and treat mastitis-causing bacteria in dairy herds.
A Comparative Study of Finite Element Method and Hybrid Finite Element Method–Spectral Element Method Approaches Applied to Medium-Frequency Transformers with Foil Windings
Authors: Pourkeivannour Siamak , van Zwieten Joost S. B. , Friedrich Léo A. J. , Curti Mitrofan , Lomonova Elena A.
Published: December 2023

This study aims to improve the computational efficiency of the frequency domain analysis of medium-frequency transformers (MFTs) with the presence of large clearance distances and fine foil windings. The winding loss and magnetic energy in MFTs in the medium-frequency range are calculated utilizing a finite element method (FEM) using common triangular and alternative rectilinear mesh elements. Additionally, in order to improve the computational efficiency of the calculations, a spectral element method (SEM) is coupled with a FEM, thus creating a hybrid FEM–SEM formulation. In such a hybrid approach, the FEM is used to calculate the current density distribution in the two-dimensional (2D) cross-section of the foil conductors to achieve reliable accuracy, and the SEM is adopted in the nonconducting clearance distances of the winding window to reduce the system of equations. The comparative analysis of the calculated resistance and reactance of the under-study models showed that the FEM with rectilinear mesh elements and the FEM–SEM model outperformed the FEM with triangular mesh elements in terms of accuracy and computational cost. The hybrid FEM–SEM model enables a reduced system of equations for modeling the electromagnetic behavior of MFTs. This research provides valuable insights into both the computational approaches and meshing challenges in the analysis of MFTs and offers a foundation for future research on the design and optimization of MFTs.
Genetics in Ischemic Stroke: Current Perspectives and Future Directions
Authors: Zhang Ka , Loong Shaun , Yuen Linus , Venketasubramanian Narayanaswamy , Chin Hui-Lin , Lai Poh , Tan Benjamin
Published: December 2023

Ischemic stroke is a heterogeneous condition influenced by a combination of genetic and environmental factors. Recent advancements have explored genetics in relation to various aspects of ischemic stroke, including the alteration of individual stroke occurrence risk, modulation of treatment response, and effectiveness of post-stroke functional recovery. This article aims to review the recent findings from genetic studies related to various clinical and molecular aspects of ischemic stroke. The potential clinical applications of these genetic insights in stratifying stroke risk, guiding personalized therapy, and identifying new therapeutic targets are discussed herein.
Scalp Melanoma: A High-Risk Subset of Cutaneous Head and Neck Melanomas with Distinctive Clinicopathological Features
Authors: Palacios-Diaz Rodolfo David , de Unamuno-Bustos Blanca , Pozuelo-Ruiz Mónica , Morales-Tedone Enrico Giorgio , Ballester-Sánchez Rosa , Botella-Estrada Rafael
Published: December 2023

Scalp melanomas (SM) have been previously associated with poor overall and melanoma-specific survival rates. The aim of this study was to describe and compare the clinicopathological characteristics and survival outcomes of SM and non-scalp cutaneous head and neck melanoma (CHNM). An observational multi-center retrospective study was designed based on patients with CHNM followed in two tertiary care hospitals. A hundred and fifty-two patients had CHNM, of which 35 (23%) had SM. In comparison with non-scalp CHNM, SM were more frequently superficial spreading and nodular subtypes, had a thicker Breslow index median (2.1 mm vs. 0.85 mm), and a higher tumor mitotic rate (3 vs. 1 mitosis/mm2) (p < 0.05). SM had a higher risk of recurrence and a higher risk of melanoma-specific death (p < 0.05). In the multivariate analysis, scalp location was the only prognostic factor for recurrence, and tumor mitotic rate was the only prognostic factor for melanoma-specific survival. We encourage routinely examining the scalp in all patients, especially those with chronic sun damage.
Liver Dysfunction with Severe Cholestasis and Coagulation Disorders in the Course of Hemolytic Disease of the Newborn Requiring Chelation Therapy—A Case Report and Review of the Literature
Authors: Drozdowska-Szymczak Agnieszka , Proczka Julia , Chrzanowska-Liszewska Danuta , Truszkowski Krzysztof , Mazanowska Natalia , Krajewski Paweł
Published: December 2023

Severe hemolytic disease of the fetus and newborn (HDFN) requiring intrauterine transfusions (IUTs) may cause iron accumulation, resulting in liver damage, which may lead to cholestasis and coagulation disorders. In this article, we reported a case of a female neonate who underwent chelation therapy with a positive outcome, and we reviewed the English and Polish literature on chelation therapy in HDFN available in PubMed. The patient with maximum ferritin concentration above 33,511.2 ng/mL developed liver dysfunction with coagulation disorders requiring multiple transfusions of fresh frozen plasma (FFP), Octaplex® and cryoprecipitate, and hypoalbuminemia treated with numerous albumin infusions. Furthermore, severe cholestasis was observed with direct bilirubin levels up to 33.14 mg/dL. Additionally, the child developed transient myelosuppression with neutropenia, thrombocytopenia, and low reticulocyte count due to several blood transfusions. The differential diagnosis tests were conducted to rule out any causes of hepatic failure other than hemolytic disease of the newborn. This case proves that adequate treatment of severe HDFN with anemia requiring IUT and hepatic failure can lead to positive outcomes with no long-term consequences.
Combined Inositols, α-Lactalbumin, Gymnema Sylvestre and Zinc Improve the Lipid Metabolic Profile of Patients with Type 2 Diabetes Mellitus: A Randomized Clinical Trial
Authors: Nani Alessandro , Bertuzzi Federico , Meneghini Elena , Mion Elena , Pintaudi Basilio
Published: December 2023

Type 2 diabetes mellitus (T2DM) is characterized by high blood glucose levels and lipid alterations. Besides pharmacological treatment, lifestyle modifications and nutraceuticals can be used to manage glucose and lipid profiles, which is crucial for preventing, or avoiding, serious consequences associated with the condition. This randomized controlled clinical trial on 75 patients with T2DM evaluated the effects of a combination of myo-inositol and d-chiro-inositol (40:1), α-lactalbumin, Gymnema sylvestre, and zinc on glucose and lipid profile. The intention-to-treat analysis displayed no significant differences in glucose parameters between the groups; however, the study group displayed reduced levels of total cholesterol (p = 0.01) and LDL (p = 0.03) after 3 months of supplementation. A subgroup analysis involving patients who did not modify their antidiabetic therapy, after 6 months displayed improved levels of total cholesterol (p = 0.03) and LDL (p = 0.04) in the study group versus placebo, along with a greater body weight reduction (p = 0.03) after 3 months. Furthermore, within the study group, levels of HDL (p = 0.03) and triglycerides (p = 0.04) improved after 3 months. These findings support supplementation with myo-inositol and d-chiro-inositol (40:1), α-lactalbumin, Gymnema sylvestre, and zinc as an adjuvant and safe strategy to manage the lipid profiles of patients with T2DM.
Should Tinnitus Patients with Subclinical Hearing Impairment Be Offered Hearing Aids? A Comparison of Tinnitus Mitigation Following 3 Months Hearing Aid Use in Individuals with and without Clinical Hearing Impairment
Authors: Waechter Sebastian , Olovsson Maria , Pettersson Petter
Published: December 2023

There is a consensus among tinnitus experts to not recommend hearing aids for tinnitus patients with subclinical hearing impairment. However, this notion is arbitrary, as no previous study has compared the treatment effect of hearing aids on tinnitus distress in patients with and without clinical hearing impairment. In this article, we investigate whether tinnitus patients with clinical and subclinical hearing impairment differ in terms of tinnitus mitigation after hearing aid fitting. Twenty-seven tinnitus patients with either clinical (n = 13) or subclinical (n = 14) hearing impairment were fitted with hearing aids. All participants filled out the tinnitus functional index (TFI) before hearing aid fitting and after 3 months of hearing aid use. Clinically meaningful reductions in tinnitus distress (−13 TFI points or more) were seen in both groups, and the difference in tinnitus mitigation between tinnitus patients with clinical (mean TFI reduction = 17.0 points) and subclinical hearing impairment (mean TFI reduction = 16.9 points) was not statistically significant (p = 0.991). Group differences on the suspected confounding factors of age, sex, time since tinnitus debut, tinnitus distress (TFI score) at baseline, and treatment adherence were statistically insignificant. In light of this, we argue that clinical hearing impairment is not required to achieve meaningful tinnitus mitigation with hearing aids, and that hearing aids could be recommended for tinnitus patients with subclinical hearing impairment.
Geriatric Nutritional Risk Index Predicts High Activities of Daily Living at Discharge in Older Patients with Heart Failure after Cardiac Rehabilitation
Authors: Muraoka Yuta , Miura Takahiro , Miyagi Midori , Okazaki Tatsuma , Katsumata Taiki , Obata Keisuke , Ebihara Satoru
Published: December 2023

Background: Hospitalization often leads to a decline in activities of daily living (ADL) in older patients with heart failure. Although cardiac rehabilitation (CR) improves ADL, it can be difficult to perform CR due to the deconditioning of these patients. This study aimed to examine the factors associated with ADL at discharge in older patients with heart failure who underwent CR. Methods: A total of 86 of 110 older heart failure patients aged ≥ 75 years (average age, 86.9 ± 5.7 years) transferred to our institution for CR were enrolled and classified into high ADL at discharge (n = 54) and low ADL at discharge (n = 32) groups. Physical characteristics, comorbidities, medications, blood test data, echocardiographic data, and nutritional status (Geriatric Nutritional Risk Index [GNRI]) were retrospectively examined from medical records. ADL were assessed using the Barthel Index (BI) at admission and discharge. Considering multicollinearity, the relationship between high ADL (BI ≥ 60) at discharge and these assessments at admission was analyzed using multiple logistic regression analysis. The receiver operating characteristic curve was analyzed to calculate the cutoff values for the parameters identified by the multiple logistic regression analysis. Results: The GNRI was the only independent factor predicting high ADL at discharge (p = 0.041; odds ratio [OR], 1.125; 95% confidence interval [CI], 1.005–1.260). The area under the receiver operating characteristic curve for the GNRI was 0.770 (95% CI, 0.664–0.876). The cutoff value for the GNRI was 83.4 (sensitivity, 85.2%; specificity, 62.5%). Conclusion: These findings suggest that the GNRI score at admission predicts high ADL at discharge in older patients with heart failure who underwent CR.
Intrahospital Transport of Critically Ill Patients with Subarachnoid Hemorrhage—Frequency, Timing, Complications, and Clinical Consequences
Authors: Schmidbauer Moritz L. , Wiegand Tim L. T. , Keidel Linus , Zibold Julia , Dimitriadis Konstantinos
Published: December 2023

Background: Patients with subarachnoid hemorrhage (SAH) often necessitate intra-hospital transport (IHT) during intensive care treatment. These transfers to facilities outside of the neurointensive care unit (NICU) pose challenges due to the inherent instability of the hemodynamic, respiratory, and neurological parameters that are typical in these patients. Methods: In this retrospective, single-center cohort study, a total of 108 IHTs were analyzed for demographics, transport rationale, clinical outcomes, and pre/post-IHT monitoring parameters. After establishing clinical thresholds, the frequency of complications was calculated, and predictors of thresholds violations were determined. Results: The mean age was 55.7 (+/−15.3) years, with 68.0% showing severe SAH (World Federation of Neurosurgical Societies Scale 5). IHTs with an emergency indication made up 30.8% of all transports. Direct therapeutic consequences from IHT were observed in 38.5%. On average, the first IHT occurred 1.5 (+/−2.0) days post-admission and patients were transported 4.3 (+/−1.8) times during their stay in the NICU. Significant parameter changes from pre- to post-IHT included mean arterial pressure, systolic blood pressure, oxygen saturation, blood glucose levels, temperature, dosages of propofol and ketamine, tidal volume, inspired oxygen concentration, Horovitz index, glucose, pH, intracranial pressure, and cerebral perfusion pressure. Relevant hemodynamic thresholds were violated in 31.5% of cases, while respiratory complications occurred in 63.9%, and neurological complications in 20.4%. For hemodynamic complications, a low heart rate with a threshold of 61/min (OR 0.96, 95% CI 0.93–0.99, p = 0.0165) and low doses of midazolam with a threshold of 17.5 mg/h (OR 0.97, 95% CI 0.95–1.00, p = 0.0232) significantly predicted adverse events. However, the model did not identify significant predictors for respiratory and neurological outcomes. Conclusions: Conclusively, IHTs in SAH patients are associated with relevant changes in hemodynamic, respiratory, and neurological monitoring parameters, with direct therapeutic consequences in 4/10 IHTs. These findings underscore the importance of further studies on the clinical impact of IHTs.
Psychological Screening, Standards and Spinal Cord Injury: Introducing Change in NHS England Commissioned Services
Authors: Duff Jane , Ellis Rebecca , Kaiser Sally , Grant Lucy C
Published: December 2023

Psychologist resourcing across the United Kingdom (UK) spinal cord injury centres (SCICs) varies considerably, which has detrimentally impacted standardising service provision for people with spinal cord injuries/disorders (PwSCI/D) compared with other nations. This paper presents the outcome of a project involving the Spinal Cord Injury Psychology Advisory Group (SCIPAG) and NHS England Clinical Reference Group/SCI transformation groups to agree upon screening and standards and shares data from the National Spinal Injuries Centre (NSIC) and the Yorkshire and Midlands Regional SCICs. Inpatients completed the GAD-7, the PHQ-9, and the short form of the Appraisals of DisAbility: Primary and Secondary Scale (ADAPSSsf), assessing adjustment. A total of 646 participants were included, with 43% scoring above the clinical threshold on at least one of the measures on admission. A subset of 272 participants also completed discharge measures and 42% remained above the threshold on discharge, demonstrating sustained psychological need. This paper provides support for services to move to a screen-and-assessment model supplemented by referral options for those with changing needs or who present with difficulties outside the remit of screening. The findings also support the efficacy of universal screening across the system and consideration of screening and standards for psychological care by the wider psychology community.