bluuebunny/crossref_metadata_2025 huggingface dataset:
An Overview of Histamine and Other Biogenic Amines in Fish and Fish Products
Authors: Visciano Pierina , Schirone Maria , Paparella Antonello
Published: December 2020

The occurrence of biogenic amines in fish is directly associated with microorganisms with decarboxylase activity. These compounds are generally detoxified by oxidases in the intestinal tract of humans, but some conditions, such as alcohol consumption, enzyme deficiency, or monoamino-oxidase antidepressant use, can make their intake by food dangerous. Due to its toxicity, histamine is the unique biogenic amine with regulatory limits for fishery products. This review focuses on biogenic amines in fish, with a detailed picture of the number of alert notifications or intoxication events reported in the last years. The favoring conditions for their formation, as well as the main preventive and control measures to ensure public health, are also reviewed.
Consumption Context Effects on Fine Wine Consumer Segments’ Liking and Emotions
Authors: Danner Lukas , Johnson Trent E. , Ristic Renata , Meiselman Herbert L. , Bastian Susan E.P.
Published: December 2020

Wine consumer lifestyle segmentation has been widely studied; however, most studies have solely utilised online surveys. This work investigated the impact of context on wine consumer segments’ liking and emotions while consuming wines in different environments. Two studies were conducted with regular wine consumers segmented based on their fine wine behaviour using the Fine Wine Instrument. Study 1 (n = 122) investigated the effects of wine variety and product information, and Study 2 (n = 346) the effects of wine quality and consumption context, on hedonic and emotional responses of the segments. Within both studies, three segments were identified and named: Wine Enthusiasts, Aspirants and No Frills. The Wine Enthusiast segment generally liked the wines more and perceived more intense positive emotions when consuming wine compared to the No Frills segment, with the Aspirant’s likes and emotion intensities ranging in between. Wine Enthusiasts were more discriminative of their preferred wines and reported stronger positive emotions when tasting higher quality (Study 1) and more complex (Study 2) wines. The consistent results across the two studies showed for the first time that consumer segments, based on lifestyle segmentation, differ in their hedonic and emotional responses towards wine when actually tasting wines, demonstrating that the Fine Wine Instrument has practical implications and can identify wine consumers displaying different wine consumption behaviours.
TaYS1A, a Yellow Stripe-Like Transporter Gene, Is Required for Wheat Resistance to Puccinia striiformis f. sp. Tritici
Authors: Islam Md Ashraful , Guo Jia , Peng Huan , Tian Shuxin , Bai Xingxuan , Zhu Haochuan , Kang Zhensheng , Guo Jun
Published: December 2020

Yellow stripe-like (YSL) transporters are required for the transportation of metal-phytosiderophores and are structurally related to metal-nicotianamine complexes. Some studies also reported the involvement of YSL transporters in pathogen-induced defense. However, the molecular mechanisms of YSL genes involved in biotic stress responses are still not clear, especially in cereal crops. This study aimed to functionally characterize TaYS1A during the interaction of wheat and Puccinia striiformis f. sp. tritici (Pst), the causal agent of stripe rust disease. TaYS1A was localized in the cell membrane of wheat protoplasts and Nicotiana benthamiana cells. TaYS1A was significantly up-regulated in wheat leaves after being infected with the avirulent Pst isolate CYR23 and after treatment with salicylic acid (SA). Silencing of TaYS1A by the virus-induced gene silencing method enhanced the susceptibility of wheat to Pst accompanied by reducing the accumulation of SA and H2O2 and down-regulating the transcriptions of TaPR1 and TaPR2. In addition, TaYS1A was found to interact with TaNH2, a homolog of OsNH2, by yeast-two-hybrid assay, and silencing of TaYS1A diminished the expression of TaNH2. Our findings suggested the existence of positive regulation of TaYS1A in providing resistance against Pst by modulating SA-induced signaling and offered new insight into the biological role of YSL in wheat against pathogens.
A Tangle of Genomic Aberrations Drives Multiple Myeloma and Correlates with Clinical Aggressiveness of the Disease: A Comprehensive Review from a Biological Perspective to Clinical Trial Results
Authors: Sessa Mariarosaria , Cavazzini Francesco , Cavallari Maurizio , Rigolin Gian Matteo , Cuneo Antonio
Published: December 2020

Multiple myeloma (MM) is a genetically heterogeneous disease, in which the process of tumorigenesis begins and progresses through the appearance and accumulation of a tangle of genomic aberrations. Several are the mechanisms of DNA damage in MM, varying from single nucleotide substitutions to complex genomic events. The timing of appearance of aberrations is well studied due to the natural history of the disease, that usually progress from pre-malignant to malignant phase. Different kinds of aberrations carry different prognostic significance and have been associated with drug resistance in some studies. Certain genetic events are well known to be associated with prognosis and are incorporated in risk evaluation in MM at diagnosis in the revised International Scoring System (R-ISS). The significance of some other aberrations needs to be further explained. Since now, few phase 3 randomized trials included analysis on patient’s outcomes according to genetic risk, and further studies are needed to obtain useful data to stratify the choice of initial and subsequent treatment in MM.
Technologies for Pharmacogenomics: A Review
Authors: van der Lee Maaike , Kriek Marjolein , Guchelaar Henk-Jan , Swen Jesse J.
Published: December 2020

The continuous development of new genotyping technologies requires awareness of their potential advantages and limitations concerning utility for pharmacogenomics (PGx). In this review, we provide an overview of technologies that can be applied in PGx research and clinical practice. Most commonly used are single nucleotide variant (SNV) panels which contain a pre-selected panel of genetic variants. SNV panels offer a short turnaround time and straightforward interpretation, making them suitable for clinical practice. However, they are limited in their ability to assess rare and structural variants. Next-generation sequencing (NGS) and long-read sequencing are promising technologies for the field of PGx research. Both NGS and long-read sequencing often provide more data and more options with regard to deciphering structural and rare variants compared to SNV panels—in particular, in regard to the number of variants that can be identified, as well as the option for haplotype phasing. Nonetheless, while useful for research, not all sequencing data can be applied to clinical practice yet. Ultimately, selecting the right technology is not a matter of fact but a matter of choosing the right technique for the right problem.
Investigating Tradeoffs between Agricultural Development and Environmental Flows under Climate Change in the Stung Chinit Watershed, Cambodia
Authors: Bresney Susan R. , Forni Laura , Mautner Marina R. L. , Huber-Lee Annette , Shrestha Manish , Moncada Angélica M. , Ghosh Emily , Sopharith Tes
Published: December 2020

The interlinkages between water for irrigation and for fish habitat are complex. This is particularly true in the Stung Chinit, a tributary to one of the most robust fisheries in the world, where livelihoods rely heavily on rice production and fishing and there is pressure to increase rice production with increased irrigation. This study assesses the tradeoffs between various management options and irrigation strategies in the Stung Chinit watershed under multiple projections of climate change. Due to the relative demands for instream flows and rice, if dry season rice is widely promoted, flows will be severely impacted. However, implementing a flow requirement protects these flows, while only causing minor shortages to rice when planted once or twice per year. These shortages may be alleviated with improved cooperation, management and shifting rice irrigation practices. While climate change will lead to warming temperatures and potentially higher demands for irrigation, the larger threat to rice and ecosystems appears to be water management (or lack thereof). This study suggests that there is sufficient water in the system to expand the irrigated area by 10%, grow rice twice per year and protect downstream flows under climate change; however, well-coordinated management is required to achieve this.
Using UAV Based 3D Modelling to Provide Smart Monitoring of Road Pavement Conditions
Authors: Roberts Ronald , Inzerillo Laura , Di Mino Gaetano
Published: December 2020

Road pavements need adequate maintenance to ensure that their conditions are kept in a good state throughout their lifespans. For this to be possible, authorities need efficient and effective databases in place, which have up to date and relevant road condition information. However, obtaining this information can be very difficult and costly and for smart city applications, it is vital. Currently, many authorities make maintenance decisions by assuming road conditions, which leads to poor maintenance plans and strategies. This study explores a pathway to obtain key information on a roadway utilizing drone imagery to replicate the roadway as a 3D model. The study validates this by using structure-from-motion techniques to replicate roads using drone imagery on a real road section. Using 3D models, flexible segmentation strategies are exploited to understand the road conditions and make assessments on the level of degradation of the road. The study presents a practical pipeline to do this, which can be implemented by different authorities, and one, which will provide the authorities with the key information they need. With this information, authorities can make more effective road maintenance decisions without the need for expensive workflows and exploiting smart monitoring of the road structures.
Lanthanide-Based Single-Molecule Magnets Derived from Schiff Base Ligands of Salicylaldehyde Derivatives
Authors: Gebrezgiabher Mamo , Bayeh Yosef , Gebretsadik Tesfay , Gebreslassie Gebrehiwot , Elemo Fikre , Thomas Madhu , Linert Wolfgang
Published: December 2020

The breakthrough in Ln(III)-based SMMs with Schiff base ligands have been occurred for the last decade on account of their magnetic behavior, anisotropy and relaxation pathways. Herein, we review the synthetic strategy, from a structural point of view and magnetic properties of mono, di, tri and polynuclear Ln(III)-based single-molecule magnets mainly with Schiff bases of Salicylaldehyde origin. Special attention has been given to some important breakthroughs that are changing the perspective of this field with a special emphasis on slow magnetic relaxation. An overview of 50 Ln(III)-Schiff base complexes with SMM behavior, covering the period 2008–2020, which have been critical in understanding the magnetic interactions between the Ln(III)-centers, are presented and discussed in detail.
On Cancer, COVID-19, and CT Scans: A Monocentric Retrospective Study
Authors: Martini Francesca , D’Alessio Andrea , Bracchi Federico , Di Mauro Daniela , Fargnoli Anna , Motta Marco , Giussani Cristina , Meazza Prina Marco , Gobbin Giovanni , Taverna Monica
Published: December 2020

Background The use of computed tomography (CT) for coronavirus disease 2019 (COVID-19) diagnosis in an area of northern Italy with a high incidence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection may have identified more patients with this disease than RT-PCR in the very early onset of the COVID-19 pandemic. Methods We retrospectively reviewed 148 chest CT scans of oncological patients who were referred to the Radiological Unit of Policlinico S. Marco from 1 February 2020 to 30 April 2020, during the COVID-19 outbreak in Bergamo area. In parallel, we analyzed RT-PCR tests of these 148 patients. Results Among 32 patients with a diagnosis of COVID-19, 17 patients were asymptomatic or had mild symptoms (53.1%), while 15 developed severe disease (46.8%). The incidence of SARS-CoV-2 infection was 22.9%, the mortality rate was 18.8%. We did not find any correlation between disease severity and age, sex, smoking, or cardiovascular comorbidities. Remarkably, patients who were on treatment for cancer developed a milder disease than patients who were not on treatment. Conclusions The acceptance of CT-defined diagnoses in COVID-19 high-incidence areas like Bergamo region highlighted a larger oncological population affected by COVID-19 than RT-PCR, in particular, asymptomatic and mildly symptomatic patients, because only symptomatic patients underwent nasopharyngeal swabbing at the onset of the COVID-19 pandemic. We observed that patients actively treated for their cancer had a milder disease, in agreement with previous studies that suggested a protective role of immunosuppression. Admittedly, the sample of patients in our study was heterogeneous regarding the oncological disease, their prognosis, and the type of treatment; therefore, other studies are needed to confirm our data.
Environmentally Induced Epigenetic Transgenerational Inheritance and the Weismann Barrier: The Dawn of Neo-Lamarckian Theory
Authors: Nilsson Eric E. , Maamar Millissia Ben , Skinner Michael K.
Published: December 2020

For the past 120 years, the Weismann barrier and associated germ plasm theory of heredity have been a doctrine that has impacted evolutionary biology and our concepts of inheritance through the germline. Although August Weismann in his 1872 book was correct that the sperm and egg were the only cells to transmit molecular information to the subsequent generation, the concept that somatic cells do not impact the germline (i.e., the Weismann barrier) is incorrect. However, the doctrine or dogma of the Weismann barrier still influences many scientific fields and topics. The discovery of epigenetics, and more recently environmentally induced epigenetic transgenerational inheritance of phenotypic variation and pathology, have had significant impacts on evolution theory and medicine today. Environmental epigenetics and the concept of epigenetic transgenerational inheritance refute aspects of the Weismann barrier and require a re-evaluation of both inheritance theory and evolution theory.
Perspective: Controlling Epidermal Terminal Differentiation with Transcriptional Bursting and RNA Bodies
Authors: Wotherspoon Duncan , Rogerson Clare , O’Shaughnessy Ryan F.L.
Published: December 2020

The outer layer of the skin, the epidermis, is the principal barrier to the external environment: post-mitotic cells terminally differentiate to form a tough outer cornified layer of enucleate and flattened cells that confer the majority of skin barrier function. Nuclear degradation is required for correct cornified envelope formation. This process requires mRNA translation during the process of nuclear destruction. In this review and perspective, we address the biology of transcriptional bursting and the formation of ribonuclear particles in model organisms including mammals, and then examine the evidence that these phenomena occur as part of epidermal terminal differentiation.
Nanostructured Carbonated Hydroxyapatite Associated to rhBMP-2 Improves Bone Repair in Rat Calvaria
Authors: Schneider Werner Vianna Thiago , Sartoretto Suelen Cristina , Neves Novellino Alves Adriana Terezinha , Figueiredo de Brito Resende Rodrigo , de Almeida Barros Mourão Carlos Fernando , de Albuquerque Calasans-Maia Jose , Martinez-Zelaya Victor R. , Malta Rossi Alexandre , Granjeiro Jose Mauro , Calasans-Maia Monica Diuana , Seabra Louro Rafael
Published: December 2020

Many biomaterials are used for Bone Morphogenetic Proteins (BMPs) delivery in bone tissue engineering. The BMP carrier system’s primary function is to hold these growth factors at the wound’s site for a prolonged time and provide initial support for cells to attach and elaborate the extracellular matrix for bone regeneration. This study aimed to evaluate the nanostructured carbonated hydroxyapatite microspheres (nCHA) as an rhBMP-2 carrier on rats calvaria. A total of fifteen male Wistar rats were randomly divided into three groups (n = 5): clot (control group), rhBMP-2 associated with collagen membrane (COL/rhBMP-2) or associated with the microspheres (nCHA/rhBMP-2). After 45 days, the calvaria defect samples were evaluated through histological, histomorphometric, and SR-µCT analyses to investigate new-formed bone and connective tissue volume densities. The descriptive histological analysis showed that nCHA/rhBMP-2 improved bone formation compared to other groups. These results were confirmed by histomorphometric and SR-µCT analysis that showed substantially defect area filling with a higher percentage of newly formed (36.24 ± 6.68) bone than those with the COL/rhBMP-2 (0.42 ± 0.40) and Clot (3.84 ± 4.57) (p < 0.05). The results showed that nCHA is an effective carrier for rhBMP-2 encouraging bone healing and an efficient alternative to collagen membrane for rhBMP-2 delivery.
Context-Sensitive Prediction of Vessel Behavior
Authors: Steidel Matthias , Mentjes Jan , Hahn Axel
Published: December 2020

Research in the field of maritime anomaly detection and vessel behavior prediction primarily focuses on developing methods for extracting typical vessel movement patterns from historical traffic data. However, contextual information is currently not considered during pattern extraction by existing research. Combining contextual information with historical traffic data has the potential to produce both more accurate traffic patterns and more precise predictions of vessel behavior. This paper investigates the benefit of incorporating contextual information during the extraction of vessel behavior and the prediction of the most probable vessel behavior. A method is presented that combines historical vessel traffic data with information about the course of waterways. Typical behavior patterns are extracted by applying kernel density estimation, which are subsequently used for predicting the most probable vessel behavior. Using this approach, we were able to predict in which area the vessel is most likely to sail, as well as the actual track for a sailing time of 2:35 h. Additional potential applications of our approach can be derived from the results, which, in addition to behavior prediction, can also be used to detect anomalous vessel behavior.
Yeasts in Liquid Swine Diets: Identification Methods, Growth Temperatures and Gas-Formation Potential
Authors: Keller Birgit , Kuder Henrike , Visscher Christian , Siesenop Ute , Kamphues Josef
Published: December 2020

Liquid feed is susceptible to microbiological growth. Yeasts are said to cause sudden death in swine due to intestinal gas formation. As not all animals given high yeast content feed fall ill, growth and gas formation potential at body temperature were investigated as possible causally required properties. The best identification method for these environmental yeasts should be tested beforehand. Yeasts derived from liquid diets without (LD − S) and liquid diets with maize silage (LD + S) were examined biochemically (ID32C-test) and with MALDI-TOF with direct smear (DS) and an extraction method (EX). Growth temperature and gas-forming potential were measured. With MALDI-EX, most yeast isolates were identified: Candida krusei most often in LD − S, and C. lambica most often in LD + S, significantly more than in LD − S. Larger colonies, 58.75% of all yeast isolates, were formed at 25 °C rather than at 37 °C; 17.5% of all isolates did not grow at 37 °C at all. Most C. krusei isolates formed high gas amounts within 24 h, whereas none of the C. lambica, C. holmii and most other isolates did. The gas pressure formed by yeast isolates varied more than tenfold. Only a minority of the yeasts were able to produce gas at temperatures common in the pig gut.
Meta-Analysis with Few Studies and Binary Data: A Bayesian Model Averaging Approach
Authors: Vázquez-Polo Francisco-José , Negrín-Hernández Miguel-Ángel , Martel-Escobar María
Published: December 2020

In meta-analysis, the existence of between-sample heterogeneity introduces model uncertainty, which must be incorporated into the inference. We argue that an alternative way to measure this heterogeneity is by clustering the samples and then determining the posterior probability of the cluster models. The meta-inference is obtained as a mixture of all the meta-inferences for the cluster models, where the mixing distribution is the posterior model probabilities. When there are few studies, the number of cluster configurations is manageable, and the meta-inferences can be drawn with BMA techniques. Although this topic has been relatively neglected in the meta-analysis literature, the inference thus obtained accurately reflects the cluster structure of the samples used. In this paper, illustrative examples are given and analysed, using real binary data.
Impact of the Flipped Classroom Method in the Mathematical Area: A Systematic Review
Authors: Fernández-Martín Francisco-Domingo , Romero-Rodríguez José-María , Gómez-García Gerardo , Ramos Navas-Parejo Magdalena
Published: December 2020

Currently, the use of technology has become one of the most popular educational trends in Higher Education. One of the most popular methods on the Higher Education stage is the Flipped Classroom, characterised by the use of both face-to-face and virtual teaching through videos and online material, promoting more autonomous, flexible and dynamic teaching for students. In this work, we started to compile the main articles that used Flipped Classroom within the mathematical area in Higher Education, with the aim of analysing their main characteristics, as well as the impact caused on students. To do so, the method of systematic review was used, focusing on those empirical experiences published in Web of Sciences and Scopus. The results indicated that, in most cases, the implementation of Flipped Classroom led to an improvement in students’ knowledge and attitudes towards mathematical content and discipline. In addition, aspects such as collaborative work, autonomy, self-regulation towards learning or academic performance were benefited through this method.
Powder Metallurgy Processing and Mechanical Properties of Controlled Ti-24Nb-4Zr-8Sn Heterogeneous Microstructures
Authors: Fer Benoît , Tingaud David , Hocini Azziz , Hao Yulin , Leroy Eric , Prima Frédéric , Dirras Guy
Published: December 2020

This paper gives some insights into the fabrication process of a heterogeneous structured β-metastable type Ti-24Nb-4Zr-8Sn alloy, and the associated mechanical properties optimization of this biocompatible and low elastic modulus material. The powder metallurgy processing route includes both low energy mechanical ball milling (BM) of spherical and pre-alloyed powder particles and their densification by Spark Plasma Sintering (SPS). It results in a heterogeneous microstructure which is composed of a homogeneous 3D network of β coarse grain regions called “core” and α/β dual phase ultra-fine grain regions called “shell.” However, it is possible to significantly modify the microstructural features of the alloy—including α phase and shell volume fractions—by playing with the main fabrication parameters. A focus on the role of the ball milling time is first presented and discussed. Then, the mechanical behavior via shear tests performed on selected microstructures is described and discussed in relation to the microstructure and the probable underlying deformation mechanism(s).
Selenate Adsorption from Water Using the Hydrous Iron Oxide-Impregnated Hybrid Polymer
Authors: Marjanovic Vesna , Peric-Grujic Aleksandra , Ristic Mirjana , Marinkovic Aleksandar , Markovic Radmila , Onjia Antonije , Sljivic-Ivanovic Marija
Published: December 2020

Hybrid adsorbent, based on the cross-linked copolymer impregnated with hydrous iron oxide, was applied for the first time for Se(VI) adsorption from water. The influence of the initial solution pH, selenate concentration and contact time to adsorption capacity was investigated. Adsorbent regeneration was explored using a full factorial experimental design in order to optimize the volume, initial pH value and concentration of the applied NaCl solution as a reagent. Equilibrium state was described using the Langmuir model, while kinetics fitted the pseudo-first order. The maximum adsorption capacity was found to be 28.8 mg/g. Desorption efficiency increased up to 70%, and became statistically significant with the reagent concentration and pH increase, while the applied solution volume was found to be insignificant in the investigated range. Based on the results obtained, pH influence to the adsorption capacity, desorption efficiency, Fourier transform infrared (FTIR) and X-ray diffraction (XRD) analysis of loaded adsorbent, it was concluded that the outer- and inner-sphere complexation are mechanisms responsible for Se(VI) separation from water. In addition to the experiments with synthetic solutions, the adsorbent performances in drinking water samples were explored, showing the purification efficiency up to 25%, depending on the initial Se(VI) concentration and water pH. Determined sorption capacity of the cross-linked copolymer impregnated with hydrous iron oxide and its ability for regeneration, candidate this material for further research, as a promising anionic species sorbent.
The Role of Vitamin D in Small Animal Bone Metabolism
Authors: Zafalon Rafael Vessecchi Amorim , Ruberti Bruna , Rentas Mariana Fragoso , Amaral Andressa Rodrigues , Vendramini Thiago Henrique Annibale , Chacar Fernanda Chicharo , Kogika Marcia Mery , Brunetto Marcio Antonio
Published: December 2020

Dogs and cats have differences in vitamin D metabolism compared to other mammalian species, as they are unable to perform vitamin D cutaneous synthesis through sun exposure. Therefore, they are dependent on the dietary intake of this nutrient. The classic functions of vitamin D are to stimulate intestinal calcium and phosphate absorption, renal calcium and phosphate reabsorption and regulate bone mineral metabolism. Thus, it is an important nutrient for calcium and phosphorus homeostasis. This review highlights the evidence of the direct and indirect actions of vitamin D on bone mineral metabolism, the consequences of nutritional imbalances of this nutrient in small animals, as well as differences in vitamin D metabolism between different size dogs.
Composites, Fabrication and Application of Polyvinylidene Fluoride for Flexible Electromechanical Devices: A Review
Authors: Guo Shuaibing , Duan Xuexin , Xie Mengying , Aw Kean Chin , Xue Qiannan
Published: December 2020

The technological development of piezoelectric materials is crucial for developing wearable and flexible electromechanical devices. There are many inorganic materials with piezoelectric effects, such as piezoelectric ceramics, aluminum nitride and zinc oxide. They all have very high piezoelectric coefficients and large piezoelectric response ranges. The characteristics of high hardness and low tenacity make inorganic piezoelectric materials unsuitable for flexible devices that require frequent bending. Polyvinylidene fluoride (PVDF) and its derivatives are the most popular materials used in flexible electromechanical devices in recent years and have high flexibility, high sensitivity, high ductility and a certain piezoelectric coefficient. Owing to increasing the piezoelectric coefficient of PVDF, researchers are committed to optimizing PVDF materials and enhancing their polarity by a series of means to further improve their mechanical–electrical conversion efficiency. This paper reviews the latest PVDF-related optimization-based materials, related processing and polarization methods and the applications of these materials in, e.g., wearable functional devices, chemical sensors, biosensors and flexible actuator devices for flexible micro-electromechanical devices. We also discuss the challenges of wearable devices based on flexible piezoelectric polymer, considering where further practical applications could be.
Internal Microscopic Diagnosis of Accelerated Aging of Proton Exchange Membrane Water Electrolysis Cell Stack
Authors: Lee Chi-Yuan , Chen Chia-Hung , Jung Guo-Bin , Li Shih-Chun , Zeng Yi-Zhen
Published: December 2020

The hydrogen production reaction of the proton exchange membrane (PEM) water electrolysis cell stack is the reverse reaction of the fuel cell, but the water electrolysis operation requires high pressure, and the high pressure decomposes hydrogen molecules, thus aging or causing failure in the water electrolysis cell stack. In addition, there are five important physical parameters (current, voltage, flow, pressure and temperature) inside the water electrolysis cell stack, which can change the performance and shorten the life of the cell stack. However, the present techniques obtain data only by external simulation or single measurement; they cannot collect the internal real data in operation instantly and accurately. This study discusses the causes for aging or failure, and develops an internal real-time microscopic diagnosis tool for accelerated aging of the PEM water electrolysis cell stack. A flexible integrated (current, voltage, flow, pressure and temperature) microsensor applicable to the inside (high voltage and electrochemical environment) of the PEM water electrolysis cell stack is developed by using micro-electro-mechanical systems (MEMS) technology; it is embedded in the PEM water electrolysis cell stack for microscopic diagnosis of accelerated aging, and 100-h durability and reliability tests are performed. The distribution of important physical parameters inside the PEM water electrolysis cell stack can be measured instantly and accurately, so as to adjust it to the optimal operating conditions, and the local aging and failure problems are discussed.
Sheep and Cattle Are Not Susceptible to Experimental Inoculation with Hazara Orthonairovirus, a Tick-Borne Arbovirus Closely Related to CCHFV
Authors: Hartlaub Julia , von Arnim Felicitas , Fast Christine , Somova Maryna , Mirazimi Ali , Groschup Martin H. , Keller Markus
Published: December 2020

Hazara orthonairovirus (HAZV) is a tick-borne arbovirus closely related to Crimean–Congo hemorrhagic fever orthonairovirus (CCHFV). Whereas CCHFV is a biosafety level (BSL) 4 agent, HAZV is classified as BSL 2, as it is not known to cause any disease in humans. Belonging to the same serogroup as CCHFV, HAZV might act as a model which can provide a better understanding of this important zoonosis. Furthermore, the serological relatedness may cause diagnostic problems if antibodies against HAZV interfere with current CCHFV serological assays. Therefore, sheep and cattle—important natural hosts for CCHFV—were experimentally infected with HAZV to prove their susceptibility and evaluate potential antibody cross-reactivities. According to this study, neither sheep nor cattle are susceptible to experimental HAZV infections. Consequently, the HAZV infection in ruminants is clearly distinct from CCHFV infections. Sera of immunized animals weakly cross-reacted between HAZV and CCHFV in immunofluorescence and immunoblot assays, but not in commercial CCHFV ELISAs commonly used for field studies.
Mineralogy of an Appinitic Hornblende Gabbro and Its Significance for the Evolution of Rising Calc-Alkaline Magmas
Authors: Pe-Piper Georgia
Published: December 2020

The magmatic and sub-solidus evolution of calcic amphiboles and Fe–Ti oxides was investigated in the Neoproterozoic Frog Lake pluton, Nova Scotia, Canada, in order to understand the relationship between the history of hydrous magma and the resulting mineralogy. The pluton occurs as sheet-like bodies of hornblende gabbro and hornblendite, with lesser tonalite dykes and granite bodies, interlayed with screens of medium-grade metamorphic country rock. Small, diffuse clots of felsic minerals are present in the gabbro. The subsolidus growth of actinolite occurs in early clinopyroxenes and amphiboles. Ilmenite is the dominant Fe–Ti oxide, as interstitial magmatic crystals. The increase of Mn towards the margin of the ilmenite crystals indicates a gradual increase in oxygen fugacity with time, leading to the precipitation of titanite and ferrohypersthene. The replacement of titanite by ilmenite and ilmenite lamellae in the amphiboles suggests subsequent reducing conditions during the sub-solidus crystallisation. The gabbros in the coeval, but apparently shallower, Jeffers Brook granodiorite laccolith have dominant magnetite and Mg-rich subsolidus amphiboles, which are indicative of high oxygen fugacity. The differences between the two plutons suggest that there was a greater flux of hydrothermal water through the sheet-like architecture of the Frog Lake pluton.
Discovery of Novel Dual Extracellular Regulated Protein Kinases (ERK) and Phosphoinositide 3-Kinase (PI3K) Inhibitors as a Promising Strategy for Cancer Therapy
Authors: Zhang Lingzhi , Ju Qiurong , Sun Jinjin , Huang Lei , Wu Shiqi , Wang Shuping , Li Yin , Guan Zhe , Zhu Qihua , Xu Yungen
Published: December 2020

Concomitant inhibition of MAPK and PI3K signaling pathways has been recognized as a promising strategy for cancer therapy, which effectively overcomes the drug resistance of MAPK signaling pathway-related inhibitors. Herein, we report the scaffold-hopping generation of a series of 1H-pyrazolo[3,4-d]pyrimidine dual ERK/PI3K inhibitors. Compound 32d was the most promising candidate, with potent inhibitory activities against both ERK2 and PI3Kα which displays superior anti-proliferative profiles against HCT116 and HEC1B cancer cells. Meanwhile, compound 32d possessed acceptable pharmacokinetic profiles and showed more efficacious anti-tumor activity than GDDC-0980 and the corresponding drug combination (BVD-523 + GDDC-0980) in HCT-116 xenograft model, with a tumor growth inhibitory rate of 51% without causing observable toxic effects. All the results indicated that 32d was a highly effective anticancer compound and provided a promising basis for further optimization towards dual ERK/PI3K inhibitors.
Drone Brood Homogenate as Natural Remedy for Treating Health Care Problem: A Scientific and Practical Approach
Authors: Sidor Ewelina , Dżugan Małgorzata
Published: December 2020

Drone brood homogenate is a little-known bee product used in folk medicine to treat various health problems. It is a very nutritious milky substance with high content of nutrients: proteins, lipids, fatty acids, carbohydrates, vitamins (A, B, E and D), and minerals. Moreover, when collected on early stage of larvae development, it is, most of all, rich source of sex hormone (testosterone, progesterone and estradiol). Some beekeepers consider drone brood as a waste product, although in some countries they use it to fight Varroa. Meanwhile, in many scientific reports a curative effect of bee drone homogenate in treating urgent global health problems have been confirmed, including ovarian dysfunction in women and male infertility, thyroid and immunity disorders, as well as malnutrition in children. A few dietary supplements based on drone brood are available online. Many patents relating to drone brood-based dietary supplements have been filed in Russia, but their prevalence in EU countries is still limited. Further research is needed to fully recognize the pharmacological activity and increase the use of drone brood.
Antimicrobial Activity and Barrier Properties against UV Radiation of Alkaline and Enzymatically Treated Linen Woven Fabrics Coated with Inorganic Hybrid Material
Authors: Olczyk Joanna , Sójka-Ledakowicz Jadwiga , Walawska Anetta , Antecka Anna , Siwińska-Ciesielczyk Katarzyna , Zdarta Jakub , Jesionowski Teofil
Published: December 2020

One of the directions of development in the textiles industry is the search for new technologies for producing modern multifunctional products. New solutions are sought to obtain materials that will protect humans against the harmful effects of the environment, including such factors as the activity of microorganisms and UV radiation. Products made of natural cellulose fibers are often used. In the case of this type of material, it is very important to perform appropriate pretreatment before subsequent technological processes. This treatment has the aim of removing impurities from the surface of the fibers, which results in the improvement of sorption properties and adhesion, leading directly to the better penetration of dyes and chemical modifiers into the structure of the materials. In this work, linen fabrics were subjected to a new, innovative treatment being a combination of bio-pretreatment using laccase from Cerrena unicolor and modification with CuO-SiO2 hybrid oxide microparticles by a dip-coating method. To compare the effect of alkaline or enzymatic pretreatment on the microstructure of the linen woven fabrics, SEM analysis was performed. The new textile products obtained after this combined process exhibit very good antimicrobial activity against Candida albicans, significant antibacterial activity against the Gram-negative Escherichia coli and the Gram-positive Staphylococcus aureus, as well as very good UV protection properties (ultraviolet protection factor (UPF) > 40). These innovative materials can be used especially for clothing or outdoor textiles for which resistance to microorganisms is required, as well as to protect people who are exposed to long-term, harmful effects of UV radiation.
On Complex Formation between 5-Fluorouracil and β-Cyclodextrin in Solution and in the Solid State: IR Markers and Detection of Short-Lived Complexes by Diffusion NMR
Authors: Melnikova Daria L. , Badrieva Zilya F. , Kostin Mikhail A. , Maller Corina , Stas Monika , Buczek Aneta , Broda Malgorzata A. , Kupka Teobald , Kelterer Anne-Marie , Tolstoy Peter M. , Skirda Vladimir D.
Published: December 2020

In this work, the nuclear magnetic resonance (NMR) and IR spectroscopic markers of the complexation between 5-fluorouracil (5-FU) and β-cyclodextrin (β-CD) in solid state and in aqueous solution are investigated. In the attenuated total reflectance(ATR) spectra of 5-FU/β-CD products obtained by physical mixing, kneading and co-precipitation, we have identified the two most promising marker bands that could be used to detect complex formations: the C=O and C-F stretching bands of 5-FU that experience a blue shift by ca. 8 and 2 cm−1 upon complexation. The aqueous solutions were studied by NMR spectroscopy. As routine NMR spectra did not show any signs of complexation, we have analyzed the diffusion attenuation of spin–echo signals and the dependence of the population factor of slowly diffusing components on the diffusion time (diffusion NMR of pulsed-field gradient (PFG) NMR). The analysis has revealed that, at each moment, ~60% of 5-FU molecules form a complex with β-CD and its lifetime is ca. 13.5 ms. It is likely to be an inclusion complex, judging from the independence of the diffusion coefficient of β-CD on complexation. The obtained results could be important for future attempts of finding better methods of targeted anticancer drug delivery.
Radioprotective Effect of Flavonoids on Ionizing Radiation-Induced Brain Damage
Authors: Wang Qinqi , Xie Chenghao , Xi Shijun , Qian Feng , Peng Xiaochun , Huang Jiangrong , Tang Fengru
Published: December 2020

Patients receiving brain radiotherapy may suffer acute or chronic side effects. Ionizing radiation induces the production of intracellular reactive oxygen species and pro-inflammatory cytokines in the central nervous system, leading to brain damage. Complementary Chinese herbal medicine therapy may reduce radiotherapy-induced side effects. Flavonoids are a class of natural products which can be extracted from Chinese herbal medicine and have been shown to have neuroprotective and radioprotective properties. Flavonoids are effective antioxidants and can also inhibit regulatory enzymes or transcription factors important for controlling inflammatory mediators, affect oxidative stress through interaction with DNA and enhance genomic stability. In this paper, radiation-induced brain damage and the relevant molecular mechanism were summarized. The radio-neuro-protective effect of flavonoids, i.e., antioxidant, anti-inflammatory and maintaining genomic stability, were then reviewed. We concluded that flavonoids treatment may be a promising complementary therapy to prevent radiotherapy-induced brain pathophysiological changes and cognitive impairment.
MALDI MSI Reveals the Spatial Distribution of Protein Markers in Tracheobronchial Lymph Nodes and Lung of Pigs after Respiratory Infection
Authors: Do Tomas , Guran Roman , Jarosova Rea , Ondrackova Petra , Sladek Zbysek , Faldyna Martin , Adam Vojtech , Zitka Ondrej
Published: December 2020

Respiratory infections are a real threat for humans, and therefore the pig model is of interest for studies. As one of a case for studies, Actinobacillus pleuropneumoniae (APP) caused infections and still worries many pig breeders around the world. To better understand the influence of pathogenic effect of APP on a respiratory system—lungs and tracheobronchial lymph nodes (TBLN), we aimed to employ matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI). In this study, six pigs were intranasally infected by APP and two were used as non-infected control, and 48 cryosections have been obtained. MALDI-TOF MSI and immunohistochemistry (IHC) were used to study spatial distribution of infectious markers, especially interleukins, in cryosections of porcine tissues of lungs (necrotic area, marginal zone) and tracheobronchial lymph nodes (TBLN) from pigs infected by APP. CD163, interleukin 1β (IL-1β) and a protegrin-4 precursor were successfully detected based on their tryptic fragments. CD163 and IL-1β were confirmed also by IHC. The protegrin-4 precursor was identified by MALDI-TOF/TOF directly on the tissue cryosections. CD163, IL-1β and protegrin-4 precursor were all significantly (p < 0.001) more expressed in necrotic areas of lungs infected by APP than in marginal zone, TBLN and in control lungs.
Photons to Formate: A Review on Photocatalytic Reduction of CO2 to Formic Acid
Authors: Pan Hanqing , Heagy Michael D.
Published: December 2020

Rising levels of atmospheric carbon dioxide due to the burning and depletion of fossil fuels is continuously raising environmental concerns about global warming and the future of our energy supply. Renewable energy, especially better utilization of solar energy, is a promising method for CO2 conversion and chemical storage. Research in the solar fuels area is focused on designing novel catalysts and developing new conversion pathways. In this review, we focus on the photocatalytic reduction of CO2 primarily in its neutral pH species of carbonate to formate. The first two-electron photoproduct of carbon dioxide, a case for formate (or formic acid) is made in this review based on its value as; an important chemical feedstock, a hydrogen storage material, an intermediate to methanol, a high-octane fuel and broad application in fuel cells. This review focuses specifically on the following photocatalysts: semiconductors, phthalocyanines as photosensitizers and membrane devices and metal-organic frameworks.
Serum Vitamins D, B9 and B12 in Greek Patients with Inflammatory Bowel Diseases
Authors: Gioxari Aristea , Amerikanou Charalampia , Papada Efstathia , Zioga Evangelia , Georgoulis Andreas D. , Bamias George , Kaliora Andriana C.
Published: December 2020

Deficiencies in vitamin D, folate and cobalamin are common in Inflammatory Bowel Disease (IBD). The aim of the present study was to assess serum levels of these vitamins in IBD adults based on the respective serum cut off values for vitamin deficiencies, and to explore possible associations with IBD-related biomarkers and nutritional intake. A cross-sectional study was carried out and patients with Crohn’s disease (CD) or ulcerative colitis (UC) from Attica-Greece were enrolled. Medical and dietary history, clinical examination and blood/stool biomarkers were evaluated. In total, 87 patients participated in the study. Serum levels of 25(OH)D, folate and cobalamin were deficient in 36.8%, 18.4% and 5.7% of patients, respectively. Linear regression analysis in the overall patients showed positive associations between (a) serum 25(OH)D with serum iron (beta = 0.083, p = 0.005) and (b) serum cobalamin with total bilirubin (beta = 0.357, p = 0.020) and direct bilirubin (beta = 0.727, p = 0.033), adjusting for age, sex, body mass index (BMI), disease activity and duration, smoking, nutritional intake and season of recruitment. In CD patients (N = 54), a negative linear association between serum folate and fecal lysozyme was evident (beta = −0.009, p = 0.020). No associations were found for UC patients (N = 33). The serum vitamin profile may be a complementary biomarker for the evaluation of disease activity next to serum and stool inflammatory biomarkers.
Acute Effects of Red Chili, a Natural Capsaicin Receptor Agonist, on Gastric Accommodation and Upper Gastrointestinal Symptoms in Healthy Volunteers and Gastroesophageal Reflux Disease Patients
Authors: Patcharatrakul Tanisa , Kriengkirakul Chatchai , Chaiwatanarat Tawatchai , Gonlachanvit Sutep
Published: December 2020

The effects of chili on gastric accommodation (GA) in gastroesophageal reflux disease (GERD) patients have not been explored. Methods: In total, 15 healthy volunteers (HV) and 15 pH-positive non-erosive GERD (NERD) patients underwent single-photon emission computed tomography after ingesting 2 g of chili or placebo in capsules in a randomized double-blind crossover fashion with a one-week washout period. GA was the maximal postprandial gastric volume (GV) after 250 mL of Ensure® minus the fasting GV. Upper gastrointestinal symptoms were evaluated by using a visual analog scale. Results: NERD patients but not HV had significantly greater GA after chili compared to a placebo (451 ± 89 vs. 375 ± 81 mL, p < 0.05). After chili, the postprandial GVs at 10, 20, and 30 min in NERD patients were significantly greater than HV (10 min, 600 ± 73 vs. 526 ± 70 mL; 20 min, 576 ± 81 vs. 492 ± 78 mL; 30 min, 532 ± 81 vs. 466 ± 86 mL, all p < 0.05). In NERD, chili was associated with significantly less satiety, more severe abdominal burning (p < 0.05), and a trend of more severe heartburn (p = 0.06) compared to the placebo. In HV, postprandial symptoms after chili and placebo ingestion were similar (p > 0.05). Conclusions: Chili enhanced GA in NERD patients but not in HV. This suggests that the modulation of GA in NERD is abnormal and likely involves transient receptor potential vanilloid 1 (TRPV1) sensitive pathways.
Emergent Strategies for Natural Products Delivery
Authors: Bilia Anna Rita , Piazzini Vieri , Bergonzi Maria Camilla
Published: December 2020

Natural products have a wide range of applications with a profound impact in the medical and healthcare fields [...]