bluuebunny/crossref_metadata_2025 huggingface dataset:
Minimum-phase property and reconstruction of elastodynamic dereverberation matrix operators
Authors: Reinicke Christian , Dukalski Marcin , Wapenaar Kees
Published: March 2023

Minimum-phase properties are well-understood for scalar functions where they can be used as physical constraint for phase reconstruction. Existing scalar applications of the latter in geophysics include, for example the reconstruction of transmission from acoustic reflection data, or multiple elimination via the augmented acoustic Marchenko method. We review scalar minimum-phase reconstruction via the conventional Kolmogorov relation, as well as a less-known factorization method. Motivated to solve practice-relevant problems beyond the scalar case, we investigate (1) the properties and (2) the reconstruction of minimum-phase matrix functions. We consider a simple but non-trivial case of 2 × 2 matrix response functions associated with elastodynamic wavefields. Compared to the scalar acoustic case, matrix functions possess additional freedoms. Nonetheless, the minimum-phase property is still defined via a scalar function, that is a matrix possesses a minimum-phase property if its determinant does. We review and modify a matrix factorization method such that it can accurately reconstruct a 2 × 2 minimum-phase matrix function related to the elastodynamic Marchenko method. However, the reconstruction is limited to cases with sufficiently small differences between P- and S-wave traveltimes, which we illustrate with a synthetic example. Moreover, we show that the minimum-phase reconstruction method by factorization shares similarities with the Marchenko method in terms of the algorithm and its limitations. Our results reveal so-far unexplored matrix properties of geophysical responses that open the door towards novel data processing tools. Last but not least, it appears that minimum-phase matrix functions possess additional, still-hidden properties that remain to be exploited, for example for phase reconstruction.
Human norovirus contamination challenge in fresh produce: a global prevalence and meta-analytic assessment
Authors: Ekundayo Temitope C , Ijabadeniyi Oluwatosin A
Published: March 2023

Aims

Among fresh produce (FP)-associated foodborne infections, human noroviruses (HNoV) are the leading causative agent. The present study aimed to assess the prevalence of HNoV in FP.

Methods and Results

FP-associated HNoV records mined from 5 repositories until 25/05/2022 according to PRISMA standards were appraised, fitted to a generalized linear mixed-effects model and subgroup analysed by sample type, genogroup, and geographical location. Further, heterogeneity was assessed in the model coupled with a leave-one-out-cross-validation. The overall prevalence of HNoV in FP was 9.3% (95% confidence interval [CI] 3.7–21.7. HNoV prevalence was higher in fruits (9.8%[3.7–23.5]) than vegetables (9.4%[3.1–25.3]). Regarding geographical distribution, FP-associated HNoV prevalence varied from Africa (25.8%, 5.7–66.7), Asia (23.2%[4.6–65.0]), Europe (5.6%[0.7–33.9]), North America (12.4%[1.8–52.7]) to South America (7.5%[0.0–100.0]). HNoV GI overall prevalence in FP was 5.3% (2.5–11.1) and varied by sample type (fruits: 4.9%[1.7–13.2]; vegetables: 5.8%[1.6–18.8]). While the occurrence of GII in FP was 1.7% (0.7–3.9) and varied by sample type (fruits: 2.5%[1.0–6.5]; vegetables: 0.9%[0.2–4.9]), GIGII (detection of both GI and GII) was 0.3% (0.1–1.0) with 0.4% (0.1–2.2) and 0.2% (0.0–1.5) in fruits and vegetables, respectively. In all cases, the test for sample/regional prevalence differences were not significantly different with the exception of GII regional prevalence differences (p = 0.03).

Conclusions

This study suggests that FP represents a critical vehicle for HNoV dissemination, a global and a high risk for public health.
Investigation of some endophytic fungi from five medicinal plants with growth promoting ability on maize (Zea maysL.)
Authors: Hatamzadeh Sareh , Rahnama Kamran , White James Francis , Oghaz Nima Akbari , Nasrollahnejad Saeed , Hemmati Khodayar
Published: March 2023

Aims

This study aimed to identify endophytic fungi from Anthemis altissima, Matricaria parthenium, Cichorium intybus, Achillea millefolium, and A. filipendulina with plant-promoting ability on the ZP684 maize hybrid-cultivar.

Methods and results

Plants were collected from northeast-Iran and endophytic fungi were isolated and identified using partial large subunit nrDNA, internal transcribed spacer, translation elongation factor, and β-tubulin genetic markers. Endophytic fungi that improved seed germination were studied under greenhouse conditions. Ninety-seven endophytic fungi were identified. Preussia africana, Bjerkandera adusta, Schizophyllum commune, Alternaria embellisia, Trichaptum biforme, Septoria malagutii, A. consortiale, Verticillium dahliae, Fusarium avenacearum, and Trametes versicolor significantly improved seed-germination. Alternaria consortiale produced the highest level of indole-3-acetic acid-like compounds and maize growth-promoting.

Conclusions

Plant fungal colonization frequency increased with orthometric height. Sampling location Chahar Bagh at 2230 m contained the most endophytic fungi. Fusarium and Alternaria were the most frequently isolated endophytic genera. Therefore, medicinal plants are potential hosts for endophytic fungi that may be suitable biofertilizer agents in agriculture.

Significance and impact of the study

This study helps to better understand the ecosystem functions by investigating of endophytic fungi distribution under different ecological conditions. Finding effective isolates among these microorganisms with a suitable plant-promoting ability on crops may help to reduce the use of chemical fertilizers in an agroecosystem.
Culture ofMycobacterium aviumsubsp.paratuberculosis: challenges, limitations and future prospects
Authors: Dane Hannah , Stewart Linda D , Grant Irene R
Published: March 2023

Mycobacterium avium subsp. paratuberculosis (MAP) causes paratuberculosis (Johne's disease) in ruminants and is suspected to be involved in the development of Crohn's disease and several autoimmune disorders. As such, sensitive and specific MAP detection methods are required to confirm infection in animals and identify potential sources of animal and human exposure. Despite recent developments in immunological and nucleic acid-based detection methods, culture-based detection of MAP remains the ‘gold standard’ against which the sensitivity and specificity of other detection methods are measured. However, not all culture-based approaches are equivalent in terms of detection capability, which can lead to errors in the evaluation of other detection methods. This review will provide an overview of the chronological development of culture methods for MAP, and will consider the unique growth requirements of MAP, the merits of solid versus liquid culture media, the relative performance of the commonly used MAP culture media, and sample preparation/decontamination protocols for different sample types. The limitations of current MAP culture methods and prospects for improvements are discussed.
Bacterial interactions with the mycelium of the cultivated edible mushroomsAgaricus bisporusandPleurotus ostreatus
Authors: Shamugam Shivaghami , Kertesz Michael A
Published: March 2023

The cultivated edible mushrooms Agaricus bisporus and Pleurotus ostreatus are valuable food crops and an important source of human nutrition. Agaricus bisporus is the dominant cultivated species in the western hemisphere and in Australia, while in Asian countries P. ostreatus is more prevalent. These two mushroom species are grown on fermented-pasteurized substrates, and bacteria and fungi play an important role in converting feedstocks into a selective medium for the mushroom mycelium. The mushrooms are usually introduced to the substrate as grain spawn, and the actively growing hyphae form a range of direct interactions with the diverse bacterial community in the substrate. Of these interactions, the most well studied is the removal of inhibitory volatile C8 compounds and ethylene by pseudomonads, which promotes mycelium growth and stimulates primordia formation of both A. bisporus and P. ostreatus. Bacterial biomass in the substrate is a significant nutrition source for the A. bisporus mycelium, both directly through bacteriolytic enzymes produced by A. bisporus, and indirectly through the action of extracellular bacterial enzymes, but this is less well studied for P. ostreatus. Apart from their role as a food source for the growing mycelium, bacteria also form extensive interactions with the mycelium of A. bisporus and P. ostreatus, by means other than those of the removal of inhibitory compounds. Although several of these interactions have been observed to promote mycelial growth, the proposed mechanisms of growth promotion by specific bacterial strains remain largely uncertain, and at times conflicting. Bacterial interactions also elicit varying growth-inhibitory responses from A. bisporus and P. ostreatus. This review explores characterized interactions involving bacteria and A. bisporus, and to a lesser degree P.ostreatus, and whilst doing so identifies existing research gaps and emphasizes directions for future work.
Bioremediation of hexavalent chromium by transformation ofEscherichia coliDH5α with chromate reductase (ChrR) genes ofPseudomonas putidaisolated from tannery effluent
Authors: Sundarraj Shenbagamoorthy , Sudarmani D N P , Samuel Ponpandian , Sevarkodiyone S P
Published: March 2023

Aims

Hexavalent chromium (Cr(VI)), a toxic heavy metal, is a serious pollutant from tannery effluent, and its accumulation in soil and water causes severe environmental concerns and increasing public health issues. The present study focuses on the isolation and identification of chromium-reducing bacteria collected from the tannery industry in Dindigul, Tamil Nadu. Chromium-reducing bacteria Pseudomonas putida were identified by 16S rRNA sequencing followed by BLAST search. The plasmid with Cr(VI) reductase gene was isolated from Ps. putida and transferred to Escherichia coli DH5α for further studies.

Methods and Results

The bacterial cultures were kept under controlled conditions for 72 h to observe the growth rates and bacterial resistance to chromium. When strains wild-type and transformant E. coli DH5α were grown in chromium-supplemented media, they revealed significant growth, but strains cured type Ps. putida and E. coli DH5α recorded minimum growth. The Cr(VI) reduction employed by transformant E. coli DH5α and wild Ps. putida was 42.52 ± 1.48% and 44.46 ± 0.55%, respectively. The culture supernatant of the wild Ps. putida and transformant E. coli DH5α showed an increased reduction of Cr(VI) compared with cell extract supernatant and cell debris due to the extracellular activity of chromium reductase being responsible for Cr(VI) reduction. Besides, the chromium reductase gene was confirmed in the isolated Ps. putida and transformant E. coli DH5α.

Conclusions

Transformant bacteria could employ an alternative method for heavy metal detoxification in contaminated environments like tannery effluent and mining processes.

Significance and Impact of Study

High Cr(VI) concentration resistance and high Cr(VI) reducing the strain's ability make it suitable for bioremediation. These possible horizontal gene transfer events indicated in this study may have enabled transformant E. coli DH5α as a good candidate for reducing the heavy metal pollution.
Changes of lipids composition in different ontogenetic stages ofThecaphora frezii: expression of key enzymes for lipid biosynthetic pathways
Authors: Díaz María S , Figueroa Ana C , Alasino Valeria R , Turco Mauricio , Fernández Andrea , Marino Bibiana , Soria Néstor W , Beltramo Dante M
Published: March 2023

Aims

It is known that Thecaphora frezii produces peanut smut that generates numerous economic losses. For this reason, it is a priority to search for control strategies. In this sense, we investigated the lipid profile of this pathogen, as possible antifungal targets, regarding polar lipid composition, fatty acid profile, and transcriptional regulation of genes involved in each stage of the development.

Method and Results

Lipids from T. frezii teliospores, basidiospores, and hyphae were analyzed by HPLC/CAD and CG/FID. We found differences in the unsaturation levels as well as in the long-chain fatty acids along the stages. Phosphatidylcholine was the main component in the three development stages, followed by cardiolipins. Phosphatidylinositol, phosphatidylethanolamine, and lyso-phosphatidylethanolamine were found in similar amounts in all stages. Although ergosterol was not detected, we found two unsaponifiable lipids. In addition, we found transcripts that encode 28 enzymes involved in the biosynthesis of three lipids by RNA-Seq.

Conclusions

Thecaphora frezii shows changes in the composition of membrane lipids in different ontogenetic stages as well as in the expression of transcripts for enzymes involved in lipid biosynthesis.
The mechanism of Qingwen Gupi decoction on pulmonary fibrosis based on metabolomics and intestinal flora
Authors: Gao Chen , Chang Hong , Wang Zixuan , Jia Min , Li Qian , Li Xiang , Shi Songli , Bu He
Published: March 2023

Aims

To evaluate the effects of the Qingwen Gupi decoction (QGT) in a rat model of bleomycin-induced pulmonary fibrosis (PF), and explore the underlying mechanisms by integrating UPLC-Q-TOF/MS metabolomics and 16S rDNA sequencing of gut microbiota.

Methods and Results

The animals were randomly divided into the control, PF model, pirfenidone-treated, and low-, medium-, and high-dose QGT groups. The lung tissues were examined and the expression of TGF-β, SMAD-3, and SMAD-7 mRNAs in the lung tissues were analyzed. Metabolomic profiles were analyzed by UPLC-QTOF/MS, and the intestinal flora were examined by prokaryotic 16 rDNA sequencing. Pathological examination and biochemical indices revealed that QGT treatment improved the symptoms of PF by varying degrees. Furthermore, QGT significantly downregulated TGF-β1 and Smad-3 mRNAs and increased the expression levels of Smad-7. QGT-L in particular increased the levels of 18 key metabolic biomarkers that were associated with nine gut microbial species and may exert antifibrosis effects through arachidonic acid metabolism, glycerophospholipid metabolism, and phenylalanine metabolism.

Conclusions

QGT alleviated PF in a rat model through its anti-inflammatory, antioxidant, and anti-fibrotic effects, and by reversing bleomycin-induced gut dysbiosis.This study lays the foundation for further research on the pathological mechanisms of PF and the development of new drug candidates.
Soil bacterial community dynamics in plots managed with cover crops and no-till farming in the Lower Mississippi Alluvial Valley, USA
Authors: Firth A G , Brooks J P , Locke M A , Morin D J , Brown A , Baker B H
Published: March 2023

Aims

Assess bacterial community changes over time in soybean (Glycine max) crop fields following cover crop (CC) and no-till (NT) implementation under natural abiotic stressors.

Method and results

Soil bacterial community composition was obtained by amplifying, sequencing, and analysing the V4 region of the 16S rRNA gene. Generalized linear mixed models were used to assess the effects of tillage, CC, and time on bacterial community response. The most abundant phyla present were Acidobacteria, Actinobacteria, Bacteroidetes, and Verrucomicrobia. Bacterial diversity increased in periods with abundant water. Reduced tillage (RT) increased overall bacterial diversity, but NT with a CC was not significantly different than RT treatments under drought conditions. CCs shifted abundances of Firmicutes and Bacteroidetes depending on abiotic conditions.

Conclusions

In the Lower Mississippi Alluvial Valley (LMAV), USA, NT practices lower diversity and influence long-term community changes while cover crops enact a seasonal response to environmental conditions. NT and RT management affect soil bacterial communities differently than found in other regions of the country.
The antibiotic resistome inEscherichia coliisolated from human, food, and animal sources
Authors: Rodrigues Isadora de Alcântara , Ferrari Rafaela Gomes , Panzenhagen Pedro , Pereira dos Santos Anamaria Mota , Rodrigues Grazielle Lima , Junior Carlos Adam Conte , Mano Sergio Borges
Published: March 2023

Aims

The aim of this study was to analyze and compare the prevalence and distribution of resistance genes in Escherichia coli genomes isolated from human clinical samples and animal-based foods worldwide.

Methods and results

We download from NCBI Pathogen Detection Database the corresponding metadata of the 7,123 E. coli genome to access the information about the antimicrobial resistance gene content. The geographic location and the source of isolation were also obtained and compiled with the antimicrobial resistance gene for statistical analysis, results and discussion. Our criteria considered four groups for analyzing the antimicrobial resistance gene distribution. The first group of genomes from invasive clinical human (ICH) samples from countries with Human Development Index (HDI) ≥ 0.850; the second group of ICH from countries with an HDI ≤ 0.849; the third group of animal-based foods (ABF) from countries with HDI ≥ 0.850 and the fourth group of ABFs from countries with HDI ≤ 0.849. The most prevalent genes in the first group were blaCTX-M-134 (96.53%) and blaCTX-M-27 (86.35%). In the second group, ere(A) (95.96%), soxS (94.49%), qepA8 (90.81%), blaCTX-M-15 (85.66%), and fosA3 (80.88%). In the third group, the most frequently detected were aadA12 (98.5%), ant(3”) (89.92%), and blaCARB-2 (87.2%). In the fourth group, aadA12 and aac(3)-IV were identified in 100% of the analyzed genomes.

Conclusions

It was clear that the use of aminoglycosides in animal production is increasing the selective pressure on micro-organisms in both groups of countries since genes linked to aminoglycoside resistance are related to E. coli from ABF samples. The genomic profile of E. coli from HDI ≥ 0.850 countries indicates a selective pressure aimed at cephalosporins given the high prevalence in both sources.
Impact of textile dyes on human health and bioremediation of textile industry effluent using microorganisms: current status and future prospects
Authors: Sudarshan Shanmugam , Harikrishnan Sekar , RathiBhuvaneswari Govindarajan , Alamelu Venkatesan , Aanand Samraj , Rajasekar Aruliah , Govarthanan Muthusamy
Published: March 2023

Environmental contamination brought on by the discharge of wastewater from textile industries is a growing concern on a global scale. Textile industries produce a huge quantity of effluents containing a myriad of chemicals, mostly dyes. The discharge of such effluents into the aquatic environment results in pollution that adversely affects aquatic organisms. Synthetic dyes are complex aromatic chemical structures with carcinogenic and mutagenic properties in addition to high biological oxygen demand (BOD) and chemical oxygen demand (COD). This complex aromatic structure resists degradation by conventional techniques. The bioremediation approach is the biological clean-up of toxic contaminants from industrial effluents. Biological treatment methods produce less or no sludge and are cost-effective, efficient, and eco-friendly. Microorganisms, mostly microalgae and bacteria, and, in some instances, fungi, yeast, and enzymes decolorize textile dye compounds into simple, non-toxic chemical compounds. Following a thorough review of the literature, we are persuaded that microalgae and bacteria might be one of the potential decolorizing agents substituting for most other biological organisms in wastewater treatment. This article presents extensive literature information on textile dyes, their classification, the toxicity of dyes, and the bioremediation of toxic textile industry effluent utilizing microalgae and bacteria. Additionally, it combines data on factors influencing textile dye bioremediation, and a few suggestions for future research are proposed.
Engineering volatile thiol formation in yeast
Authors: Graf Felix M R , Buchhaupt Markus
Published: March 2023

Aims

Volatile thiols are very potent aroma molecules that contribute to the aroma of many beverages. The characteristic thiols of certain wine varieties such as Sauvignon blanc are partly released during the yeast-based fermentation from plant-synthesized glutathione- or cysteine-conjugated and dipeptic precursors present in the must. In this work, we aimed at the construction and characterization of yeast strains with the ability to synthesize volatile thiols from respective precursors.

Methods and Results

Besides genome integration of the Escherichia coli gene tnaA, which encodes an enzyme with high β-lyase activity, a glutathione synthetase and glutathione-S-transferases were overexpressed. Up to 8.9 μg L−1 3-mercaptohexan-1-ol could be formed with the strain from externally added trans-2-hexen-1-ol. Well-characterized thiols such as 2-methyl-2-butanethiol, 3-mercapto-3-methylbutan-1-ol, and 8-mercapto-p-menthan-3-one, as well as several so far undescribed thiol compounds could be synthesized.

Conclusion

Volatile thiols could be produced by feeding alcohol, alkenol, aldehyde, or ketone precursors like trans-2-hexenal, trans-2-hexen-1-ol, cis-2-hexen-1-ol, 3-methyl-2-buten-1-ol, 3-buten-2-one, and pulegone to the optimized yeast cells.
Over-the-counter antibiotic dispensing: knowledge and practice of community pharmacists in the United Arab Emirates
Authors: Jairoun Sumaya , Alkhalidi Doaa Kamal , Abdulrahman Jairoun Ammar , Anbar Hanan S
Published: March 2023

Objectives

This paper aimed to assess the knowledge and practice of community pharmacists in regard to dispensing over-the-counter (OTC) antibiotics with the aim of bringing their professional knowledge up to date and promoting best practices to optimize the use of antibiotics and slow the rise in resistance.

Methods

A cross-sectional descriptive study design was used to assess the knowledge and practice of community pharmacists towards OTC Antibiotic Dispensing. Participants were issued an invitation through verified and official WhatsApp and Facebook accounts of community pharmacists residing in the United Arab Emirates to fill in a validated-web-based questionnaire. The questionnaire was composed of four sections. It aimed to gather basic demographic data, and address knowledge and practice amongst pharmacists of dispensing OTC antibiotics. Data were analysed using SPSS version 26.

Key findings

A total number of 438 participating community pharmacists in the study completed the whole questionnaire. The average knowledge score was 68.6% with a 95% confidence interval (CI) of [66.9%, 70.2%]. The average practice score was 71.4% with a 95% confidence interval (CI) of [69.5%, 73.2%].

Conclusions

It is recommended that several interventions are launched to promote improved prescribing behaviour, particularly ensuring that all pharmacies receive standardized guidelines on antibiotic use and inviting pharmacists to workshops and other training programmes dedicated to promoting rational drug use. Moreover, regulatory bodies must devise, enforce and monitor the implementation of policies governing how antibiotic prescriptions and patient consultations are managed by community pharmacists.
In situextreme mass ratio inspirals via subparsec formation and migration of stars in thin, gravitationally unstable AGN discs
Authors: Derdzinski Andrea , Mayer Lucio
Published: March 2023

We investigate the properties of stars born via gravitational instability in accretion discs around supermassive black holes (SMBHs) in active galactic nuclei (AGNs), and how this varies with the SMBH mass, accretion rate, or viscosity. We show with geometrically thin, steady-state disc solutions that fragmentation results in different populations of stars when one considers the initial conditions (e.g. density and temperature of the gravitationally unstable regions). We find that opacity gaps in discs around $10^6 \, {\rm M}_{\odot }$ SMBHs can trigger fragmentation at radii ≲ 10−2 pc, although the conditions lead to the formation of initially low stellar masses primarily at $0.1\!-\!0.5 \, {\rm M}_{\odot }$. Discs around more massive SMBHs ($M_{\rm BH} =10^{7-8} \, {\rm M}_{\odot }$) form moderately massive or supermassive stars (the majority at $10^{0-2} \, {\rm M}_{\odot }$). Using linear migration estimates, we discuss three outcomes: stars migrate till they are tidally destroyed, accreted as extreme mass ratio inspirals (EMRIs), or leftover after disc dispersal. For a single-AGN activity cycle, we find a lower limit for the EMRI rate $R_{\rm emri}\sim 0\!-\!10^{-4} \, \rm yr^{-1}$ per AGN assuming a star formation efficiency $\epsilon =1\!-\!30{{\ \rm per\ cent}}$. In cases where EMRIs occur, this implies a volumetric rate up to $0.5\!-\!10 \, \rm yr^{-1}\, Gpc^{-3}$ in the local Universe. The rates are particularly sensitive to model parameters for $M_{\rm BH}=10^6 \, {\rm M}_{\odot }$, for which EMRIs only occur if stars can accrete to 10s of solar masses. Our results provide further evidence that gas-embedded EMRIs can contribute a substantial fraction of events detectable by milliHz gravitational wave detectors such as the Laser Interferometer Space Antenna (LISA). Our disc solutions suggest the presence of migration traps, as has been found for more massive SMBH discs. Finally, the surviving population of stars after the disc lifetime leaves implications for stellar discs in galactic nuclei.
Substrate specificity of phenoloxidase-like activity in an ecoimmunological model speciesLymnaea stagnalis
Authors: Seppälä Otto , Schlegel Tamara
Published: March 2023

Ecoimmunological research on molluscs and other invertebrates frequently quantifies phenoloxidase (PO) activity to estimate the strength of the immune function. PO enzymes form different families whose relative roles in oxidative reactions are typically unknown. Understanding this could allow enzyme-specific assays with higher accuracy than in commonly used nonspecific assays. We tested the contribution of different PO enzyme families to haemolymph PO-like activity in Lymnaea stagnalis snails using substrates specific to enzymes detected in L. stagnalis transcriptome data (p-phenylenediamine, specific to laccases; L-tyrosine, specific to tyrosinases) and compared the reactions to those with a nonspecific substrate (L-dopa). We found laccase-like but no tyrosinase-like activity. However, reactions with L-dopa were the strongest, possibly due to other oxidative enzymes in snail haemolymph. Laccase-like activity is common in molluscs, and we propose the use of enzyme-specific assays in future ecoimmunological studies of this taxon. The lack of tyrosinase-like activity in L. stagnalis contradicts earlier transcriptome data, which calls for investigating the expression of PO enzymes in L. stagnalis at the proteome level.
Questionnaire survey on the prevention and development of cervical cancer in patients with systemic lupus erythematosus in Japan
Authors: Murakawa Yohko , Dobashi Hiroaki , Kondo Masahiro , Nishiyama Susumu , Okazaki Ryota , Hasegawa Yasuyuki , Moriyama Mayuko , Sugiura Tomoko , Onishi Ikuko , Honda Manabu , Nagamura Norihiro , Yoshioka Yuki , Minamoto Toshiko
Published: March 2023

Objectives

The aim is to evaluate the prevention and development of cervical cancer in systemic lupus erythematosus (SLE) patients in Japan and its background based on a questionnaire survey.

Methods

The questionnaire was handed to 460 adult female SLE patients at 12 medical institutions. The participants were grouped by age, and data related to their human papillomavirus vaccination status, age at first coitus, cervical cancer screening, and diagnosis of cervical cancer were analysed.

Results

A total of 320 responses were received. Patients aged 35–54 years included a higher proportion of patients whose age at first coitus was <20 years. This group also showed a higher rate of cervical cancer/dysplasia. Only nine patients had a human papillomavirus vaccination history. Adequate frequency of cervical cancer screening was slightly higher (52.1%) among SLE patients than in the Japanese general population. However, 23% of the patients had never undergone examination, primarily because of a feeling of troublesome. The incidence of cervical cancer was significantly higher among SLE patients. One reason for this may be associated with the use of immunosuppressants, although the difference was not significant.

Conclusions

SLE patients are at a higher risk of cervical cancer and dysplasia. Rheumatologists should proactively recommend vaccination and screening examinations for SLE female patients.
Therapeutic Assessment with Couples (TA-2)
Authors: Aschieri Filippo , Caputo Camillo , Righetti Tommaso
Published: March 2023

This chapter demonstrates the Therapeutic Assessment with Couples (TA-2) model, summarizing its rationale and the literature on its development and effects. It also reviews research on partner selection and its evolution into long-term “couple dances.” Couple dances are patterns through which partners manage their psychological conflicts at the expense of rigid, dissatisfying couple relationships. Based on the mechanisms underlying couple functioning, the chapter describes the aims and the basic steps of the TA-2. First, the couple and assessor collaboratively formulate questions to be answered through the assessment process. TA-2 toggles between individual sessions and couple sessions. Individual sessions, composed of tests and collaborative processing of the testing experience, allow partners to gain insight and develop a new comprehension of their relational problems supported by the assessor. The insights and shared understanding that emerge are discussed and reviewed in the summary session, which aims to answer both the individual’s and the couple’s assessment questions. In the end, the assessor sends the couple written feedback, including answers to their assessment questions and test results, which is discussed during the summary and discussion session. This chapter illustrates the TA-2 model with a real case in which partners dealt with intolerable affect states due to their upbringing and the consequences of severe stressors they suffered due to rigid projective identification. The couple exemplifies the “avoidant-pursuer” dance. The TA-2 allowed both partners to become aware of the origins of such dance and become more able to tolerate intimacy.
Development of a medication review tool for community-dwelling older adults in Korea
Authors: Lee Soojin , Jun Kwanghee , Lee Ah Young , Ah Young-Mi , Lee Ju-Yeun
Published: March 2023

Background

With the aging population, older adults are more likely to receive outpatient care. Therefore, it is necessary to identify drug-related problems (DRPs) and potentially inappropriate medications (PIMs) associated with adverse clinical outcomes in community-dwelling older adults. This study aimed to develop a medication review tool for community-dwelling older adults in Korea.

Methods

We developed the tool using three steps: (i) establishment of a preliminary list by reviewing 21 existing tools, (ii) a two-round Delphi survey to evaluate clinical appropriateness and (iii) a two-round Delphi survey to evaluate applicability. The list was categorized into 23 diseases/conditions with five types of DRPs. The interventions for each item have been described.

Results

The preliminary list contained 100 items. The final list contained 81 items, including 17 general PIMs, 26 PIMs under specific disease/conditions, 16 potential drug interactions, 20 potential omissions and 2 PIMs requiring dose adjustment.

Conclusion

We developed a disease-based explicit medication review tool that can be used in primary care. This tool would assist primary care healthcare providers in identifying inappropriate medication use, which may help reduce adverse clinical consequences in older adults. Further studies are required to validate the clinical efficacy of this tool.
Inclusion of spray dried plasma in diets based on different ingredient combinations increases the digestibility of energy, fiber, Ca, and P by young pigs
Authors: Bailey Hannah M , Campbell Joy M , Torres-Mendoza Leidy J , Fanelli Natalia S , Stein Hans H
Published: March 2023

An experiment was conducted to test the hypothesis that inclusion of spray dried plasma (SDP) in diets increases apparent total tract digestibility (ATTD) and/or the standardized total tract digestibility (STTD) of gross energy (GE) and nutrients in diets for young pigs, and that ATTD of energy and nutrients or STTD of P in individual ingredients are additive in diets containing SDP. Eighty barrows (body weight: 9.30 ± 0.97 kg) were housed in individual metabolism crates and allotted to 1 of 10 diets in a randomized complete block design with 8 replicate pigs per diet. Four diets were prepared without SDP and contained ingredients commonly used in the United States, Canada, the European Union, or Asia. Four additional diets were prepared by mixing 94% of the previous four diets and 6% SDP. A diet containing SDP as the sole source of P and a P-free diet were also formulated. The ATTD of GE and nutrients and the STTD of P were calculated in all diets except the P-free diet and for the four regional diets containing 6% SDP, values were also predicted from the digestibility obtained in SDP alone and the regional diets without SDP. Differences between measured and predicted values for digestibility of GE and nutrients were also calculated. An interaction was observed between SDP and region for the ATTD of soluble dietary fiber where the digestibility decreased (P < 0.05) for pigs fed the U.S. diet with 6% SDP compared with 0% SDP, but that was not the case for the other regional diets. There was no interaction for the ATTD of GE, N, insoluble dietary fiber (IDF), total dietary fiber (TDF), Ca, and P or the STTD of P, but the ATTD and STTD values were greater (P < 0.05) or tended to be greater (P < 0.10%) when 6% SDP was included in the diet compared with diets with 0% SDP. The ATTD of GE, IDF, TDF, and P, and the STTD of P was greater (P < 0.05) for the Asia diet compared with the other diets regardless of inclusion of SDP. The measured ATTD of IDF and TDF was greater (P < 0.05) than the predicted values for the U.S. and European Union diets, and the measured ATTD of GE, N, Ca, and P and the STTD of P was greater (P < 0.05) than the predicted values for the Asia diet. In conclusion, addition of 6% SDP to a diet will increase the ATTD of energy and nutrients and the STTD of P in diets for weanling pigs, and in some cases, the measured ATTD of energy and nutrients or the STTD of P by pigs fed diets containing SDP is greater than predicted from individual ingredients.
Treatment of Recurrent Pressure Injury Using an Allograft Adipose Matrix
Authors: Melnychuk Igor , Thompson Cecily
Published: March 2023

A 77-year-old man with a more than 10-year history of a spinal cord injury developed bilateral trochanteric stage 3 pressure injuries (PIs) several years ago. They initially healed. The right trochanteric PI opened again and continued to reopen every 2 to 3 months, likely because of deficient adipose layer in the area of the healed PI.

To treat the recurrent PI, providers injected a total of 3 mL of allograft adipose matrix into the ulcerated area of the right trochanter PI in a fanning fashion to increase subcutaneous cushioning over the bony prominence. Silicone foam was used to assist with pressure reduction for the first month. When the ulcerations healed at 1 month, the silicone foam was discontinued, and an emollient ointment was applied bilaterally to provide both the currently affected site and healed scar tissue with moisture and enhanced barrier function. Follow-up examinations were completed at 1, 3, 7, 11, 14, 16, 19, 22, and 24 months; the ulcerations remained closed, and no new PIs developed.

The authors propose that allograft adipose matrix is a potential treatment modality for recurrent PIs needing a supplemented subcutaneous layer that other modalities cannot provide. Further use is ongoing in clinical scenarios when there is deficient adipose layer such as recurrent PIs or to prevent PI deterioration in early stages.
Women Veterans’ Descriptions of Interactions With Civilian Health Care Providers
Authors: Vance Billie S. , Carpenter Roger
Published: March 2023

Women veterans often obtain care for unique gender- and military-related health issues from civilian health care settings. There is a gap in the literature surrounding woman veterans’ perspectives about the care they receive. The purpose of this study was to apply the Interaction Model of Client Health Behavior framework to study the patient-provider interaction described by women veterans. A qualitative descriptive methodology, using directed content analysis, was employed. Six themes were identified. Consistent screening, provider knowledge about service roles and experiences of women veterans, and familiarity with military-related health conditions support holistic care. Further research in this area is warranted.
Management of pregnancy in left ventricular assist device and heart transplant recipients: a concise review
Authors: Marek-Iannucci Stefanie , Uber Patricia , Rajapreyar Indranee
Published: March 2023

Purpose of review

Women of reproductive age are increasingly undergoing heart transplantation (HT) or left ventricular assist device (LVAD) implantation for advanced heart failure. This review is intended to give an overview of the current state of the art management of pregnancy in patients with LVAD or HT recipients.

Recent findings

Heart transplant recipients are at increased risk for graft rejection, renal dysfunction, preeclampsia and worsening of comorbidities (hypertension and diabetes). Patients with LVAD are at higher risk of thromboembolic events, infections, right ventricular failure and require close surveillance during pregnancy. Preconception counseling must be offered to all women of reproductive age group with HT or LVAD to avoid unplanned pregnancies.

A multidisciplinary approach with close antepartum and postpartum surveillance is recommended.
Management of hypertension in patients supported with continuous flow left ventricular assist devices
Authors: Krishnarao Krithika , Krim Selim R.
Published: March 2023

Purpose of review

Hypertension remains one of the most common clinical problems leading to devastating postleft ventricular assist device (LVAD) implant complications. This study reviews the pathophysiology of hypertension in the setting of continuous flow LVAD support and provides an update on currently available antihypertensive therapies for LVAD patients.

Recent findings

The true prevalence of hypertension in the LVAD population remains unknown. Effective blood pressure (BP) control and standardization of BP measurement are key to prevent suboptimal left ventricular unloading, pump malfunction and worsening aortic regurgitation. Angiotensin-converting enzyme inhibitors (ACEI), angiotensin receptor blockers (ARB), beta blockers and mineralocorticoid receptor antagonists (MRA) are the preferred antihypertensive agents because of their additional potential benefits, including optimization of haemodynamics, prevention of stroke, gastrointestinal bleed and in some patients myocardial recovery. Angiotensin receptor-neprilysin inhibition (ARNI) may be a well tolerated and effective therapy for BP control especially among CF-LVAD patients with resistant hypertension. Similarly, sodium glucose co-transporter 2 inhibitors (SGLT2i) should be considered in the absence of contraindications.

Hypertension is very common post-LVAD implant. Heart failure guideline directed medical therapies, including ACEI, ARB, beta blockers and MRA, are the preferred antihypertensive agents to improve post-LVAD outcomes.
Bilateral Intraorbital Opticmeningoceles in Joubert Syndrome
Authors: Cruz Antonio Augusto V. , Feltrini Talissa , Chahud Fernando , Messias Katharina
Published: March 2023

Congenital opticmeningoceles was the term coined to describe large pseudocystic lesions of the intraorbital segment of the optic nerve. This extremely rare congenital anomaly was reported unilaterally only in nonsyndromic patients with fully developed eyes. The authors describe here a 10-month-old girl with a previous diagnosis of Joubert syndrome who presented with the same type of optic nerve malformation in OU. Molecular genetic analysis disclosed a pathogenic variant of the TMEM67 gene which is associated with various types of ciliopathies.
Effectiveness of Community Health Worker-Led Interventions in Enhancing Colorectal Cancer Screening Uptake in Racial and Ethnic Minority Populations
Authors: Rana Tika , Chan Dorothy N. S. , Nguyen Khanh T. , Choi Kai C. , So Winnie K. W.
Published: March 2023

Background

The colorectal cancer (CRC) screening uptake rate is substantially lower in ethnic minority populations than in the general population. Racial and ethnic minority individuals experience more barriers in obtaining a screening test for CRC when compared with the non-Hispanic White population.

Objective

To examine the effectiveness of community health worker–led interventions in improving the CRC screening uptake rate in racial and ethnic minority populations.

Methods

Five databases, EMBASE, CINAHL, MEDLINE, Scopus, and PubMed, were systematically searched, and reference lists of the identified articles were manually searched for relevant articles in May 2022. Only randomized controlled trials were included.

Results

A total of 10 randomized controlled trials conducted in the United States were included in this review. The findings of the meta-analysis showed that CRC screening uptake was enhanced in participants receiving community health worker–led interventions compared with those receiving no intervention (odds ratio, 2.25; 95% confidence interval, 1.48-3.44; P < .001). The subgroup analysis by diverse racial and ethnic groups and number of components (single vs multiple) of the community health worker–led interventions showed that multicomponent interventions were more effective in increasing the CRC uptake rate among all racial and ethnic groups regardless of their background.

Conclusions

Multicomponent community health worker–led interventions can improve CRC screening uptake in racial and ethnic minority populations.

Implications for Practice

The findings of the present review show that multicomponent community health worker–led interventions are shown to be effective to improve the CRC screening uptake targeting other racial and ethnic minority groups in other countries.
Determination of the Relationship Between Gynecological Cancer Awareness and Fear of Cancer in Women Using Social Media and the Affecting Factors
Authors: Aydın Ruveyde , Karakısla Fatmanur Sena , Kabukcuoğlu Kamile
Published: March 2023

Background

Understanding the relationship between fear of gynecological cancer and awareness of cancer in women may help oncology nurses increase women’s participation in gynecological cancer screening.

Objective

To examine the relationship between women’s gynecological cancer awareness and cancer fear using social media and the affecting factors.

Methods

In a descriptive and cross-sectional study design, online data were collected from 1231 women aged 20 to 69 years through social media using the Descriptive Information Form, the Gynecological Cancer Awareness Scale (GCAS), and the Cancer Worry Scale (CWS). Analytic tests included paired t tests, analysis of variance, and post hoc correlations and structural equation modeling.

Results

The women’s total mean GCAS score was above moderate (152 ± 17), and their mean CWS score was high (19 ± 5). There was no relationship between the GCAS and CWS mean scores. Characteristics of participants with high awareness of gynecological cancer were being young, having a high educational level, having children, living in a province, having a history of cancer or family history of cancer, and having regular gynecological examinations, Papanicolaou smear tests, breast self-examination, and vulva examinations (P < .05). The fear of cancer was higher in women with children, a family history of cancer, and regular gynecological examination behaviors (P < .05).

Conclusion

Awareness of gynecological cancer was not significantly statistically associated with fear of cancer. Certain sociodemographic and gynecological characteristics are associated with awareness of gynecological cancer and/or fear of cancer.

Implications for Practice

Oncology nurses may plan individualized or large-scale educational events that may make use of social media to enhance accurate awareness of gynecological cancer and related examinations in women.
Factors affecting successful prosthetic use after hemipelvectomy and hip disarticulation amputations: Five-year experience from a tertiary prosthetic rehabilitation center
Authors: Saif Ahmad , Francis Richard , Sullivan John , Sedki Imad
Published: March 2023

Background:

Rehabilitation after hip disarticulation and hemipelvectomy amputations can be challenging, and many opt to forego prosthetic limb use. There is limited evidence on characteristics that result in successful prosthetic use; therefore, our study aimed to identify these to help guide patient expectations.

Methods:

A retrospective review was performed on patients seen at a UK tertiary prosthetic center in the past 5 years with hip disarticulation or hemipelvectomy amputations. Details and etiology of amputation, length between assessment and delivery of prosthesis, goals, reasons for abandonment, and outcomes were recorded. Successful prosthetic use was defined as the use of a prosthesis at least 3 times a week. Characteristics were compared using odds ratios and Fisher exact test.

Results:

Twenty-seven patient notes were analyzed: 42% female, 58% male, and age range 14-90. Thirty percent had a hemipelvectomy, and 70% had a hip disarticulation. Neoplasia accounted for 78% of etiologies followed by trauma 15% and infected endoprosthesis 7%. Sixty-seven percent trialed a walking prosthesis; 33% of these stopped eventually. There were no statistically significant findings of factors increasing odds of successful prosthetic use. However, age significantly increased the odds of being given a trial of a prosthesis.

Conclusion:

Although younger patients are more likely to be given the opportunity to trial a walking prosthesis, age does not seem to affect the overall outcome alone. In cases of neoplasia, there is often a delayed start to rehabilitation and prosthetic use, which may affect eventual success. Further studies are required to define the optimum characteristics for successful prosthetic use at higher amputation levels.