bluuebunny/crossref_metadata_2025 huggingface dataset:
Risk Factors for Testicular Cancer: Environment, Genes and Infections—Is It All?
Authors: Yazici Sertac , Del Biondo Dario , Napodano Giorgio , Grillo Marco , Calace Francesco Paolo , Prezioso Domenico , Crocetto Felice , Barone Biagio
Published: April 2023

The incidence of testicular cancer is steadily increasing over the past several decades in different developed countries. If on one side better diagnosis and treatment have shone a light on this disease, on the other side, differently from other malignant diseases, few risk factors have been identified. The reasons for the increase in testicular cancer are however unknown while risk factors are still poorly understood. Several studies have suggested that exposure to various factors in adolescence as well as in adulthood could be linked to the development of testicular cancer. Nevertheless, the role of environment, infections, and occupational exposure are undoubtedly associated with an increase or a decrease in this risk. The aim of this narrative review is to summarize the most recent evidence regarding the risk factors associated with testicular cancer, starting from the most commonly evaluated (cryptorchidism, family history, infections) to the newer identified and hypothesized risk factors.
Process Parameter Optimization of 2507 Super Duplex Stainless Steel Additively Manufactured by the Laser Powder Bed Fusion Technique
Authors: Mulhi Ali , Dehgahi Shirin , Waghmare Prashant , Qureshi Ahmed J.
Published: April 2023

Laser powder bed fusion is an attractive technology for producing high-strength stainless steel alloys. Among the stainless steels, 2507 super duplex stainless steel (2507 SDSS) is known for its excellent combination of corrosion resistance and high strength. Although there are some studies that aimed at optimizing the laser powder bed fusion (LPBF) printing parameters to print highly dense 2507 SDSS parts; However, a full optimization study is not reported yet. This study aims at optimizing the printing parameters for 2507 SDSS, namely: laser power, scan speed, and hatch distance. The response surface methodology was used in generating a detailed design of experiment to investigate the different pore formation types over a wide energy density range (22.22–428.87 J/mm3), examine the effects of each process parameter and their interactions on the resulting porosity, and identify an optimized parameter set for producing highly dense parts. Different process parameters showed different pore formation mechanisms, with lack-of-fusion, metallurgical or gas, and keyhole regimes being the most prevalent pore types identified. The lack-of-fusion pores are observed to decrease significantly with increasing the energy density at low values. However, a gradual increase in the keyhole pores was observed at higher energy densities. An optimal energy density process window from 68.24 to 126.67 J/mm3 is identified for manufacturing highly dense (≥99.6%) 2507 SDSS parts. Furthermore, an optimized printing parameter set at a laser power of 217.4 W, a scan speed of 1735.7 mm/s, and a hatch distance of 51.3 µm was identified, which was able to produce samples with 99.961% relative density. Using the optimized parameter set, the as-built 2507 SDSS sample had a ferrite phase fraction of 89.3% with a yield and ultimate tensile strength of 1115.4 ± 120.7 MPa and 1256.7 ± 181.9 MPa, respectively.
Investigation on Initial Shell Solidification and the Effect of Negative Strip Time on Oscillation Marks during Continuous Casting
Authors: Cao Minghui , Liu Yuanhe , Zhang Xingzhong
Published: April 2023

The initial solidification of the shell and the effect of the negative strip on oscillation marks were studied during the oscillation of the mold. A two-dimensional model was established concerning mold oscillation, which was coupled with fluid flow, heat transfer, and solidification, and the validity of the model was verified. The results show that oscillation marks were formed at the negative strip stage and that the quality of the slabs can be improved by reducing the duration of the negative strip stage. During the negative strip stage, the shell was affected by the strong backflow of liquid slag and the pressure on the surface sharply increased, resulting in the formation of a depression oscillation mark on the shell. The effects of the negative strip stage on the initial solidified shell during each cycle were compared. As the depth of the oscillation mark decreased, the upward shear stress on the shell’s surface increased, without the occurrence of a negative strip stage during one cycle. The results provided a new method for reducing oscillation marks and are of great significance for improving casting slabs’ quality.
Circulation of SARS-CoV–Related Coronaviruses and Alphacoronaviruses in Bats from Croatia
Authors: Brnić Dragan , Lojkić Ivana , Krešić Nina , Zrnčić Vida , Ružanović Lea , Mikuletič Tina , Bosilj Martin , Steyer Andrej , Keros Tomislav , Habrun Boris , Jemeršić Lorena
Published: April 2023

Bats are natural hosts of various coronaviruses (CoVs), including human CoVs, via an assumed direct zoonotic spillover or intermediate animal host. The present study aimed to investigate the circulation of CoVs in a bat colony in the Mediterranean region of Croatia. Guano and individual droppings from four bat species were sampled and tested with the E-gene sarbecovirus RT-qPCR, the pan-CoV semi-nested RT-PCR targeting the RdRp gene and NGS. Furthermore, bat blood samples were investigated for the presence of sarbecovirus-specific antibodies with the surrogate virus neutralization test (sVNT). The initial testing showed E-gene Sarebeco RT-qPCR reactivity in 26% of guano samples while the bat droppings tested negative. The application of RdRp semi-nested RT-PCR and NGS revealed the circulation of bat alpha- and betaCoVs. Phylogenetic analysis confirmed the clustering of betaCoV sequence with SARS-CoV–related bat sarbecoviruses and alpha-CoV sequences with representatives of the Minunacovirus subgenus. The results of sVNT show that 29% of bat sera originated from all four species that tested positive. Our results are the first evidence of the circulation of SARS-CoV–related coronaviruses in bats from Croatia.
Potential of Molecular Culture in Early Onset Neonatal Sepsis Diagnosis: A Proof of Principle Study
Authors: Dierikx Thomas , Budding Andries , Bos Martine , van Laerhoven Henriëtte , van der Schoor Sophie , Niemarkt Hendrik , Benninga Marc , van Kaam Anton , Visser Douwe , de Meij Tim
Published: April 2023

Delay in the time-to-positivity of a peripheral blood culture (PBC), the gold standard for early onset neonatal sepsis (EOS) diagnosis, has resulted in excessive use of antibiotics. In this study, we evaluate the potential of the rapid Molecular Culture (MC) assay for quick EOS diagnosis. In the first part of this study, known positive and spiked blood samples were used to assess the performance of MC. In the in vivo clinical study, the second part of this study, all infants receiving antibiotics for suspicion of EOS were included. At initial EOS suspicion, a blood sample was collected for PBC and MC. MC was able to detect bacteria present in the spiked samples even when the bacterial load was low. In the clinical study, MC was positive in one infant with clinical EOS (Enterococcus faecalis) that was not detected by PBC. Additionally, MC was positive in two infants without clinical sepsis (Streptococcus mitis and multiple species), referred to as contamination. The other 37 samples were negative both by MC and PBC. MC seems to be able to detect bacteria even when the bacterial load is low. The majority of MC and PBC results were comparable and the risk for contamination and false positive MC results seems to be limited. Since MC can generate results within 4 h following sampling compared with 36–72 h in PBC, MC may have the potential to replace conventional PBC in EOS diagnostics in order to guide clinicians on when to discontinue antibiotic therapy several hours after birth.
Knowledge, Attitudes and Behaviors regarding Tick-Borne Encephalitis Vaccination and Prevention of Tick-Borne Diseases among Primary Care Physicians in Bavaria and Baden-Wuerttemberg, Germany, May–September 2022
Authors: Coyer Liza , Sogan-Ekinci Aylin , Greutélaers Benedikt , Kuhn Julia , Saller Franziska S. , Hailer Jana , Böhm Stefanie , Brosch Rainer , Wagner-Wiening Christiane , Böhmer Merle M.
Published: April 2023

In 2020, a record number of tick-borne encephalitis (TBE) cases was reported in major endemic areas in Germany, i.e., the southern federal states of Baden-Wuerttemberg and Bavaria. Most cases were unvaccinated. Other tick-borne diseases (TBDs), including Lyme borreliosis and tularemia, are rising, too. Thus, strategies are needed to increase TBE vaccination uptake in risk areas and promote education on TBD prevention. Primary care physicians are key providers of both vaccinations and TBD education. The TBD-Prevention (TBD-Prev) study aimed to investigate the knowledge, attitudes and behaviors of primary care physicians in Baden-Wuerttemberg and Bavaria with regard to TBE vaccination and prevention of TBDs and to derive strategies for increasing vaccination rates and improving knowledge about TBE and other TBDs in the population and among primary care physicians. We invited all primary care physicians (N = 14,046) in both states to participate by mail. Using standardized, self-administered questionnaires, available both on paper and online, we asked physicians anonymously about their knowledge, attitudes and behaviors with respect to TBE vaccination and TBD prevention and their need for further information/educational materials. A total of 2321 physicians participated between May and September 2022 (response rate 17%), of whom 1222 (53%) worked in Baden-Wuerttemberg and 1067 (46%) in Bavaria. Among the participating physicians, 56% were male, 71% were >50 years and 51% worked in an individual practice. Furthermore, 91% were aware of the German national vaccination guidelines, and 98% perceived their knowledge of the risks and benefits of vaccination as adequate. A total of 97% offer TBE vaccinations, 67% provide vaccination counselling during initial consultations with new patients and 64% actively remind patients about due vaccinations. In addition, 24% expressed a need for further information materials, mainly traditional, analogue media such as flyers (82%) and posters (50%), and named timeliness, quality assurance, easy comprehensibility and independence from the pharmaceutical industry as the most important characteristics of such materials. Almost all participating physicians reported offering TBE vaccinations and feeling well-informed about TBE vaccination and TBDs. However, active offering of vaccinations and education could be further improved, and additional, low-threshold information materials are needed. Based on these results, we will develop and provide various materials on TBE vaccination and TBDs, in particular flyers and posters, for use by physicians during consultations.
New Strategy for the Design of Anti-Corrosion Coatings in Bipolar Plates Based on Hybrid Organic–Inorganic Layers
Authors: Li Xiaoxuan , Sun Wenjie , Zheng Yuhui , Long Chenggang , Wang Qianming
Published: April 2023

As a star material in conducting polymers, a polypyrrole coating was assembled onto the surface of 316 stainless steel by an electrochemical method. In the next step, the composite layer consisting of carbon nitride nanosheets (CNNS) and polymethyl methacrylate (PMMA) was sprayed. The corrosion manner of composite coatings in a simulated proton-exchange membrane fuel cell (PEMFC) environment was evaluated. The results show that the final coating generated at a voltage of 1.0 has demonstrated the optimized corrosion resistance. The polypyrrole layer improves the corrosion resistance of the stainless steel substrate, and the CNNS/PMMA coating further strengthens the physical barrier effect of the coating in corrosive solutions.
Study of the Sensing Kinetics of G Protein-Coupled Estrogen Receptor Sensors for Common Estrogens and Estrogen Analogs
Authors: Lu Dingqiang , Wang Xinqian , Feng Chunlei , Liu Danyang , Liu Yixuan , Liu Yujiao , Li Jie , Zhang Jiayao , Li Na , Deng Yujing , Wang Ke , Ren Ruijuan , Pang Guangchang
Published: April 2023

Endogenous and exogenous estrogens are widely present in food and food packaging, and high levels of natural estrogens and the misuse or illegal use of synthetic estrogens can lead to endocrine disorders and even cancer in humans. Therefore, it is consequently important to accurately evaluate the presence of food-functional ingredients or toxins with estrogen-like effects. In this study, an electrochemical sensor based on G protein-coupled estrogen receptors (GPERs) was fabricated by self-assembly, modified by double-layered gold nanoparticles, and used to measure the sensing kinetics for five GPER ligands. The interconnected allosteric constants (Ka) of the sensor for 17β-estradiol, resveratrol, G-1, G-15, and bisphenol A were 8.90 × 10−17, 8.35 × 10−16, 8.00 × 10−15, 5.01 × 10−15, and 6.65 × 10−16 mol/L, respectively. The sensitivity of the sensor for the five ligands followed the order of 17β-estradiol > bisphenol A > resveratrol > G-15 > G-1. The receptor sensor also demonstrated higher sensor sensitivity for natural estrogens than exogenous estrogens. The results of molecular simulation docking showed that the residues Arg, Glu, His, and Asn of GPER mainly formed hydrogen bonds with -OH, C-O-C, or -NH-. In this study, simulating the intracellular receptor signaling cascade with an electrochemical signal amplification system enabled us to directly measure GPER–ligand interactions and explore the kinetics after the self-assembly of GPERs on a biosensor. This study also provides a novel platform for the accurate functional evaluation of food-functional components and toxins.
Removal Performance and Mechanism of Emerging Pollutant Chloroquine Phosphate from Water by Iron and Magnesium Co-Modified Rape Straw Biochar
Authors: Sun Hongwei , He Jinjin , Liu Yucan , Ji Xianguo , Wang Gang , Yang Xiaoyong , Zhang Yanxiang
Published: April 2023

Chloroquine phosphate (CQP) is effective in treating coronavirus disease 2019 (COVID-19); thus, its usage is rapidly increasing, which may pose a potential hazard to the environment and living organisms. However, there are limited findings on the removal of CQP in water. Herein, iron and magnesium co-modified rape straw biochar (Fe/Mg-RSB) was prepared to remove CQP from the aqueous solution. The results showed that Fe and Mg co-modification enhanced the adsorption efficiency of rape straw biochar (RSB) for CQP with the maximum adsorption capacity of 42.93 mg/g (at 308 K), which was about two times higher than that of RSB. The adsorption kinetics and isotherms analysis, as well as the physicochemical characterization analysis, demonstrated that the adsorption of CQP onto Fe/Mg-RSB was caused by the synergistic effect of pore filling, π-π interaction, hydrogen bonding, surface complexation, and electrostatic interaction. In addition, although solution pH and ionic strength affected the adsorption performance of CQP, Fe/Mg-RSB still had a high adsorption capability for CQP. Column adsorption experiments revealed that the Yoon–Nelson model better described the dynamic adsorption behavior of Fe/Mg-RSB. Furthermore, Fe/Mg-RSB had the potential for repeated use. Therefore, Fe and Mg co-modified biochar could be used for the remediation of CQP from contaminated water.
Ready-to-Eat Innovative Legumes Snack: The Influence of Nutritional Ingredients and Labelling Claims in Italian Consumers’ Choice and Willingness-to-Pay
Authors: Petrontino Alessandro , Frem Michel , Fucilli Vincenzo , Labbate Antonella , Tria Emanuela , Bozzo Francesco
Published: April 2023

The global offer of legume-based snacks has sharply increased in recent years. However, to date, few studies have focused on the relationship between product supply and demand concerning the importance of attributes of such innovative foods. In this research, we identified the key internal and external determinants that affect legumes snack (LS) price and choice by Italian industries and consumers, respectively. In parallel, we investigated their preferences and perceptions towards these foods. We used the hedonic price model (HPM) and the discrete choice experiment (DCE) approach for these purposes, respectively. HPM revealed that the monetary value of LS was determined to greater significance by the: (i) size of the package; (ii) presence of rice, presence of lentils; (iii) presence of the nutritional information; and (iv) the discount shops as site of purchase. DCE revealed that the: (i) origin certification, (ii) recyclability of the package, and (iii) use of extra virgin olive oil of LS provided Italian consumers a high utility, for which they were willing to pay an average price premium of EUR 3.85, 3.64, and 1.87, respectively. On the contrary, the sunflower oil induced a decrease in their function utility. As such, this paper contributes to define potent market-segmentation strategies and to deliver effective private and public nutrition interventions for healthy eating.
Nobiletin Improves D-Galactose-Induced Aging Mice Skeletal Muscle Atrophy by Regulating Protein Homeostasis
Authors: Wang Hui-Hui , Zhang Yan , Qu Tai-Qi , Sang Xue-Qin , Li Yi-Xuan , Ren Fa-Zheng , Wen Peng-Cheng , Sun Ya-Nan
Published: April 2023

Sarcopenia, a decrease in skeletal muscle mass and function caused by aging, impairs mobility, raises the risk of fractures, diabetes, and other illnesses, and severely affects a senior’s quality of life. Nobiletin (Nob), polymethoxyl flavonoid, has various biological effects, such as anti-diabetic, anti-atherogenic, anti-inflammatory, anti-oxidative, and anti-tumor properties. In this investigation, we hypothesized that Nob potentially regulates protein homeostasis to prevent and treat sarcopenia. To investigate whether Nob could block skeletal muscle atrophy and elucidate its underlying molecular mechanism, we used the D-galactose-induced (D-gal-induced) C57BL/6J mice for 10 weeks to establish a skeletal muscle atrophy model. The findings demonstrated that Nob increased body weight, hindlimb muscle mass, lean mass and improved the function of skeletal muscle in D-gal-induced aging mice. Nob improved myofiber sizes and increased skeletal muscle main proteins composition in D-gal-induced aging mice. Notably, Nob activated mTOR/Akt signaling to increase protein synthesis and inhibited FOXO3a-MAFbx/MuRF1 pathway and inflammatory cytokines, thereby reducing protein degradation in D-gal-induced aging mice. In conclusion, Nob attenuated D-gal-induced skeletal muscle atrophy. It is a promising candidate for preventing and treating age-associated atrophy of skeletal muscles.
An Integrated Approach to Skeletal Muscle Health in Aging
Authors: Agostini Deborah , Gervasi Marco , Ferrini Fabio , Bartolacci Alessia , Stranieri Alessandro , Piccoli Giovanni , Barbieri Elena , Sestili Piero , Patti Antonino , Stocchi Vilberto , Donati Zeppa Sabrina
Published: April 2023

A decline in muscle mass and function represents one of the most problematic changes associated with aging, and has dramatic effects on autonomy and quality of life. Several factors contribute to the inexorable process of sarcopenia, such as mitochondrial and autophagy dysfunction, and the lack of regeneration capacity of satellite cells. The physiologic decline in muscle mass and in motoneuron functionality associated with aging is exacerbated by the sedentary lifestyle that accompanies elderly people. Regular physical activity is beneficial to most people, but the elderly need well-designed and carefully administered training programs that improve muscle mass and, consequently, both functional ability and quality of life. Aging also causes alteration in the gut microbiota composition associated with sarcopenia, and some advances in research have elucidated that interventions via the gut microbiota–muscle axis have the potential to ameliorate the sarcopenic phenotype. Several mechanisms are involved in vitamin D muscle atrophy protection, as demonstrated by the decreased muscular function related to vitamin D deficiency. Malnutrition, chronic inflammation, vitamin deficiencies, and an imbalance in the muscle–gut axis are just a few of the factors that can lead to sarcopenia. Supplementing the diet with antioxidants, polyunsaturated fatty acids, vitamins, probiotics, prebiotics, proteins, kefir, and short-chain fatty acids could be potential nutritional therapies against sarcopenia. Finally, a personalized integrated strategy to counteract sarcopenia and maintain the health of skeletal muscles is suggested in this review.
Mycobacterial Infections in Invasive Turtle Species in Poland
Authors: Radulski Łukasz , Krajewska-Wędzina Monika , Lipiec Marek , Weiner Marcin , Zabost Anna , Augustynowicz-Kopeć Ewa
Published: April 2023

Over the last 30 years, the number of invasive turtle species living in the wild has significantly increased in Poland. This proliferation carries many threats, which mainly include the displacement of native species of animals from their natural habitats. Turtles can also be reservoirs for pathogens, including bacteria from the Mycobacterium genus. In order to confirm or rule out the presence of acid-fast mycobacteria in the population of invasive turtle species, samples from carapace, plastron, internal organs and mouth cavity swabs from 125 animals were tested. Twenty-eight mycobacterial strains were isolated in culture, which were classified as atypical following multiplex-PCR reactions. The GenoType Mycobacterium Common Mycobacteria (CM) test, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, PCR-restriction fragment length polymorphism (PRA)-hsp65 and DNA sequencing were used to identify the species of isolates. Of the 28 strains, 11 were identified as M. fortuitum, 10 as M. chelonae, 3 as M. avium ssp. avium, 2 as M. nonchromogenicum and 1 each of M. neoaurum and M. scrofulaceum. The results of the research will also strengthen the understanding that these animals can be vectors for pathogens when living in the wild.
Lights and Dots toward Therapy—Carbon-Based Quantum Dots as New Agents for Photodynamic Therapy
Authors: Jovanović Svetlana , Marković Zoran , Budimir Milica , Prekodravac Jovana , Zmejkoski Danica , Kepić Dejan , Bonasera Aurelio , Marković Biljana Todorović
Published: April 2023

The large number of deaths induced by carcinoma and infections indicates that the need for new, better, targeted therapy is higher than ever. Apart from classical treatments and medication, photodynamic therapy (PDT) is one of the possible approaches to cure these clinical conditions. This strategy offers several advantages, such as lower toxicity, selective treatment, faster recovery time, avoidance of systemic toxic effects, and others. Unfortunately, there is a small number of agents that are approved for usage in clinical PDT. Novel, efficient, biocompatible PDT agents are, thus, highly desired. One of the most promising candidates is represented by the broad family of carbon-based quantum dots, such as graphene quantum dots (GQDs), carbon quantum dots (CQDs), carbon nanodots (CNDs), and carbonized polymer dots (CPDs). In this review paper, these new smart nanomaterials are discussed as potential PDT agents, detailing their toxicity in the dark, and when they are exposed to light, as well as their effects on carcinoma and bacterial cells. The photoinduced effects of carbon-based quantum dots on bacteria and viruses are particularly interesting, since dots usually generate several highly toxic reactive oxygen species under blue light. These species are acting as bombs on pathogen cells, causing various devastating and toxic effects on those targets.
Immobilized Keratin HPLC Stationary Phase—A Forgotten Model of Transdermal Absorption: To What Molecular and Biological Properties Is It Relevant?
Authors: Sobańska Anna Weronika , Brzezińska Elżbieta
Published: April 2023

Chromatographic retention data collected on immobilized keratin (KER) or immobilized artificial membrane (IAM) stationary phases were used to predict skin permeability coefficient (log Kp) and bioconcentration factor (log BCF) of structurally unrelated compounds. Models of both properties contained, apart from chromatographic descriptors, calculated physico-chemical parameters. The log Kp model, containing keratin-based retention factor, has slightly better statistical parameters and is in a better agreement with experimental log Kp data than the model derived from IAM chromatography; both models are applicable primarily to non-ionized compounds.Based on the multiple linear regression (MLR) analyses conducted in this study, it was concluded that immobilized keratin chromatographic support is a moderately useful tool for skin permeability assessment.However, chromatography on immobilized keratin may also be of use for a different purpose—in studies of compounds’ bioconcentration in aquatic organisms.
Evaluation of a Cardiovascular Systems Model for Design and Analysis of Hemodynamic Safety Studies
Authors: Fu Yu , Snelder Nelleke , Guo Tingjie , van der Graaf Piet H. , van Hasselt Johan. G. C.
Published: April 2023

Early prediction, quantification and translation of cardiovascular hemodynamic drug effects is essential in pre-clinical drug development. In this study, a novel hemodynamic cardiovascular systems (CVS) model was developed to support these goals. The model consisted of distinct system- and drug-specific parameter, and uses data for heart rate (HR), cardiac output (CO), and mean atrial pressure (MAP) to infer drug mode-of-action (MoA). To support further application of this model in drug development, we conducted a systematic analysis of the estimation performance of the CVS model to infer drug- and system-specific parameters. Specifically, we focused on the impact on model estimation performance when considering differences in available readouts and the impact of study design choices. To this end, a practical identifiability analysis was performed, evaluating model estimation performance for different combinations of hemodynamic endpoints, drug effect sizes, and study design characteristics. The practical identifiability analysis showed that MoA of drug effect could be identified for different drug effect magnitudes and both system- and drug-specific parameters can be estimated precisely with minimal bias. Study designs which exclude measurement of CO or use a reduced measurement duration still allow the identification and quantification of MoA with acceptable performance. In conclusion, the CVS model can be used to support the design and inference of MoA in pre-clinical CVS experiments, with a future potential for applying the uniquely identifiable systems parameters to support inter-species scaling.
Multi-Bessel Beams Generated by an Axicon and a Spatial Light Modulator for Drilling Applications
Authors: Lutz Christian , Schwarz Simon , Marx Jan , Esen Cemal , Hellmann Ralf
Published: April 2023

We report on an optical setup to generate multi-Bessel beam profiles combining a refractive axicon and a spatial light modulator. Based on their particular beam profile, Bessel beams offer advantageous properties for micro drilling processes and internal volume processing, especially for transparent materials. In addition, the laser power of industrial, ultrashort pulsed lasers has increased significantly over the last few years, offering the possibility for highly efficient processes using multi-spot profiles. Our optical concept combines the dynamic possibilities of beam splitting using a spatial light modulator with the benefits of Bessel beams, which facilitates multi-Bessel beam processing. Beside the simulation and experimental evaluation of the generated multi-Bessel beams, we exemplify the applicability of the developed module for the perforation of thin metal foils by micro drilling.
In Vitro Pro-Apoptotic and Anti-Migratory Effects of Marantodes pumilum (syn. Labisia pumila) Extracts on Human Prostate Cancer Cell Lines: Bioguided Isolation of 5-Henicosene-1-yl-resorcinol
Authors: Hanafi Mohd Mukrish Mohd , Yaakob Harisun , Gibbons Simon , Prieto Jose M.
Published: April 2023

This study aims to evaluate the in vitro cytotoxic and anti-migratory effects of Marantodes pumilum Blume Kuntze plant extracts on prostate cancer cells, identify the active compound/s, and characterize their mechanism of action. The crude methanolic extract was partitioned into n-hexane (MPh), chloroform (MPc), and aqueous (MPa) extracts. Antiproliferative fractions (IC50 < 30 μg/mL based on SRB staining of LNCaP and PC3 cell lines) were further fractionated. Active compound/s were identified using spectroscopic methods. In vitro mechanistic studies on PC3 cells included: annexin V-FITC staining, mitochondrial membrane potential (MMP) depolarization measurements, the activity of caspases 3 and 7, nuclear DNA fragmentation, cell cycle analysis, modulation of Bax, Bcl-2, Smac/Diablo, Alox-5, VEGF-A, CXCR4, and CXCL12 mRNA gene expression via RT-PCR, 2D migration (scratch assay), and 3D invasion (Boyden chamber). MPc extract was the most active, inducing cell death (p < 0.05) via apoptosis, as evidenced by nuclear DNA fragmentation and an increase in MMP depolarization (p < 0.05) as well as the activation of caspases 3/7 (MPc p < 0.01) in both PC3 and LNCaP cell lines. In addition, MPc upregulated Bax and Smac/DIABLO, downregulated Bcl-2 (p < 0.05), and inhibited ALOX-5 mRNA gene expression (p < 0.001). MPc was not cytotoxic against normal human fibroblast cells (HDFa) at the tested concentrations. Moreover, MPc inhibited migration and invasion of PC3 cells (p < 0.01). These effects were accompanied by the downregulation of both VEGF-A and CXCL-12 gene expressions (p < 0.001). A monounsaturated 5-alkyl resorcinol was isolated as the active compound in the MPc extract and identified as 5-henicosene-1-yl-resorcinol.
Isolation and Identification of Plant-Growth Inhibitory Constituents from Polygonum chinense Linn and Evaluation of Their Bioherbicidal Potential
Authors: Lun Thang Lam , Iwasaki Arihiro , Suenaga Kiyotake , Kato-Noguchi Hisashi
Published: April 2023

Polygonum chinense Linn. is a medicinal and invasive plant that belongs to the family Polygonaceae. The pharmacological activities and phytochemical constituents of Polygonum chinense are well reported, but the allelopathic effects and potent allelopathic substances of P. chinense remain to be investigated. Hence, this experiment was conducted to separate and characterize potentially allelopathic substances from an extract of the Polygonum chinense plant. The Polygonum chinense plant extracts highly suppressed the growth of cress (Lepidium sativum L.), lettuce (Lactuca sativa L.), barnyard grass (Echinochloa crusgalli (L.) P. Beauv.), and timothy grass (Phleum pratense L.) seedlings in a species- and concentration-dependent way. Two active substances were separated using a series of purification procedures and determined through spectral analysis as (−)-3-hydroxy-β-ionone and (−)-3-hydroxy-7,8-dihydro-β-ionone. These two compounds significantly suppressed the seedling growth of Lepidium sativum (cress) at concentrations of 0.01 and 1 mM, respectively. The extract concentrations necessary for 50% growth inhibition (I50 values) of the cress hypocotyls and roots were 0.05 and 0.07 mM for (−)-3-hydroxy-β-ionone, respectively, and 0.42 and 1.29 mM for (−)-3-hydroxy-7,8-β-ionone, respectively. These findings suggest that these two compounds are in charge of the inhibitory effects of the Polygonum chinense extract and may serve as weed control agents.
Biogenic Synthesis, Characterization, and In Vitro Biological Evaluation of Silver Nanoparticles Using Cleome brachycarpa
Authors: Sumra Ayesha Ahmed , Zain Maryam , Saleem Tahira , Yasin Ghulam , Azhar Muhammad Farooq , Zaman Qamar Uz , Budhram-Mahadeo Vishwanie , Ali Hayssam M.
Published: April 2023

The therapeutical attributes of silver nanoparticles (Ag-NPs) in both conditions (in vitro and in vivo) have been investigated using different plants. This study focused on the green chemistry approach that was employed to optimize the synthesis of silver nanoparticles (AgNPs) using Cleome brachycarpa aqueous extract as a reducing and stabilizing agent. The characterization of obtained CB-AgNPs was undertaken using UV-visible spectroscopy, Atomic-force microscopy (AFM), Fourier-Transform Infrared Spectroscopy (FTIR), scanning electron microscopy (SEM), and Energy-Dispersive X-ray (EDX) analysis. Results suggest that CB-AgNPs synthesized via stirring produced small-sized particles with more even distribution. The synthesized silver nanoparticles were spherical with a 20 to 80 nm size range. In vitro studies were used to analyze antioxidant, antidiabetic, and cytotoxic potential under different conditions. The results also indicated that CB-AgNPs may have significant potential as an antidiabetic in low concentrations, but also exhibited potential antioxidant activity at different concentrations. Moreover, the anticancer activity against the breast cell line (MCF-7) with IC50 reached up to 18 μg/mL. These results suggest that green synthesized silver nanoparticles provide a promising phytomedicine for the management of diabetes and cancer therapeutics.
Extraction of Diterpene-Phytochemicals in Raw and Roasted Coffee Beans and Beverage Preparations and Their Relationship
Authors: Novaes Fábio Junior Moreira , da Silva Maria Alice Esteves , Silva Diana Cardoso , Aquino Neto Francisco Radler de , Rezende Claudia Moraes
Published: April 2023

Cafestol and kahweol are expressive furane-diterpenoids from the lipid fraction of coffee beans with relevant pharmacological properties for human health. Due to their thermolability, they suffer degradation during roasting, whose products are poorly studied regarding their identity and content in the roasted coffee beans and beverages. This article describes the extraction of these diterpenes, from the raw bean to coffee beverages, identifying them and understanding the kinetics of formation and degradation in roasting (light, medium and dark roasts) as the extraction rate for different beverages of coffee (filtered, Moka, French press, Turkish and boiled). Sixteen compounds were identified as degradation products, ten derived from kahweol and six from cafestol, produced by oxidation and inter and intramolecular elimination reactions, with the roasting degree (relationship between time and temperature) being the main factor for thermodegradation and the way of preparing the beverage responsible for the content of these substances in them.
Optimization of Gel Flooding during the High Water Cut Stage in a Conglomerate Reservoir of the Xinjiang A Oilfield
Authors: Xin Xiankang , Liu Qian , Liu Saijun , Yu Gaoming , Wan Qingshan
Published: April 2023

Influenced by water injection, a dominant flow channel is easily formed in the high water cut stage of a conglomerate reservoir, resulting in the inefficient or ineffective circulation of the injected water. With gel flooding as one of the effective development methods to solve the above problems, its parameter optimization determines its final development effect, which still faces great challenges. A new optimization method for gel flooding is proposed in this paper. Firstly, the gel flooding parameters were obtained through physical experiments; then, an experimental model of gel flooding was established according to the target reservoir, and parameter sensitivity analysis was carried out. Next, a history matching of the gel flooding experiment was carried out. Finally, history matching of the target reservoir was also carried out, and a gel flooding scheme was designed and optimized to determine the best parameters. The experimental results showed that the gelation time was 4 h and the gel viscosity was 6332 mPa·s; the breakthrough pressure, resistance factor (RF), and residual resistance factor (RRF) all decreased with the increase in permeability. The gel had a good profile control ability and improved oil recovery by 16.40%. The numerical simulation results illustrated that the porosity of the high permeability layer (HPL) had the greatest impact on the cumulative oil production (COP) of the HPL, and the maximum polymer adsorption value of the HPL had the largest influence on the COP of the low permeability layer (LPL) and the water cut of both layers. Benefiting from parameter sensitivity analysis, history matching of the gel flooding experiment and a conglomerate reservoir in the Xinjiang A Oilfield with less time consumed and good quality was obtained. The optimization results of gel flooding during the high water cut stage in a conglomerate reservoir of the Xinjiang A Oilfield were as follows: the gel injection volume, injection rate, and polymer concentration were 2000 m3, 50 m3/d, and 2500 mg/L, respectively. It was predicted that the water cut would decrease by 6.90% and the oil recovery would increase by 2.44% in two years. This paper not only provides a more scientific and efficient optimization method for gel flooding in conglomerate reservoirs but also has important significance for improving the oil recovery of conglomerate reservoirs.
Polymer Composite Analysis and Characterization
Authors: Espinach Francesc X. , Tarrés Quim
Published: April 2023

In the original article [...]
In Situ Dispersion of Lignin in Polypropylene via Supercritical CO2 Extrusion Foaming: Effects of Lignin on Cell Nucleation and Foam Compression Properties
Authors: Ho Keen Hoe , Lu Xuehong , Lau Soo Khim
Published: April 2023

Supercritical CO2 (scCO2) extrusion foamed high-melt-strength (HMS) polypropylene (PP) often suffers from low cell density, large cell sizes, and poor cell structure uniformity due to the poor nucleation rates of CO2 in the PP. To remedy this, various inorganic fillers have been used as heterogeneous nucleation agents. Although their efficient nucleation effects have been demonstrated, the preparation of these fillers causes some adverse effects on the environment/human health or involves relatively expensive processes or non-eco-friendly chemicals. In this work, biomass-based lignin is studied as a sustainable, lightweight, and cost-effective nucleating agent. It is found that scCO2 could assist in situ dispersion of lignin in the PP in the foaming process, leading to significantly increased cell density, smaller cells, and improved cell uniformity. The Expansion Ratio is also simultaneously improved due to reduced diffusive gas loss. The PP/lignin foams with low lignin loadings exhibit higher compression moduli and plateau strengths than the PP foams with the same densities owing to the improved cell uniformity and probably also the reinforcing effect of the small lignin particles in cell walls. Moreover, the energy absorption capability of the PP/lignin foam with 1 wt% lignin could match the PP foam with similar compression plateau strengths; even the density of the former is 28% lower than the latter. Therefore, this work provides a promising approach to a cleaner and more sustainable production of HMS PP foams.
GF-1/6 Satellite Pixel-by-Pixel Quality Tagging Algorithm
Authors: Fan Xin , Chang Hao , Huo Lianzhi , Hu Changmiao
Published: April 2023

The Landsat and Sentinel series satellites contain their own quality tagging data products, marking the source image pixel by pixel with several specific semantic categories. These data products generally contain categories such as cloud, cloud shadow, land, water body, and snow. Due to the lack of mid-wave and thermal infrared bands, the accuracy of traditional cloud detection algorithm is unstable when facing Chinese Gaofen-1/6 (GF-1/6) data. Moreover, it is challenging to distinguish clouds from snow. In order to produce GF-1/6 satellite pixel-by-pixel quality tagging data products, this paper builds a training sample set of more than 100,000 image pairs, primarily using Sentinel-2 satellite data. Then, we adopt the Swin Transformer model with a self-attention mechanism for GF-1/6 satellite image quality tagging. Experiments show that the model’s overall accuracy reaches the level of Fmask v4.6 with more than 10,000 training samples, and the model can distinguish between cloud and snow correctly. Our GF-1/6 quality tagging algorithm can meet the requirements of the “Analysis Ready Data (ARD) Technology Research for Domestic Satellite” project.
On Evaluating the Predictability of Sea Surface Temperature Using Entropy
Authors: Jin Chang , Peng Han , Yang Hanchen , Li Wengen , Guan Jihong
Published: April 2023

Sea surface temperature (SST) has important impacts on the global ecology, and having a good understanding of the predictability, i.e., the possibility of achieving accurate prediction, of SST can help us monitor the marine environment and climate change, and guide the selection and design of SST prediction methods. However, existing studies for analyzing SST mostly measure the rising or falling trends of SST. To address this issue, we introduce a temporal-correlated entropy to quantify the predictability of SST series from both global coarse-grained and local fine-grained aspects, and make SST prediction with multiple deep learning models to prove the effectiveness of such predictability evaluation method. In addition, we explore the dynamics of SST predictability by dividing the time range of interest into consecutive time periods, evaluating the corresponding predictability of SST for each time period, and analyzing the stability of the predictability of SST over time. According to the experiments, the SST predictability values near the poles and equator are really high. The average SST predictability values of the East China Sea, Bohai Sea, and Antarctic Ocean are 0.719, 0.706, and 0.886, respectively, and the size relationship of the SST predictability in the three local sea areas is consistent with our prediction results using multiple representative SST prediction methods, which corroborates the reliability of the predictability evaluation method. In addition, we found that the SST predictability in the Antarctic Ocean changes more dramatically over time than in the East China Sea and the Bohai Sea. The results of SST predictability and its dynamic analysis indicate that global warming, ocean currents, and human activities all have significant impacts on the predictability of SST.
Effect of Excitation Signal on Double-Coil Inductive Displacement Transducer
Authors: Li Yanchao , Li Ruichuan , Yang Junru , Xu Jikang , Yu Xiaodong
Published: April 2023

A double-coil inductive displacement transducer is a non-contact element for measuring displacement and is widely used in large power equipment systems such as construction machinery and agricultural machinery equipment. The type of coil excitation signal has an impact on the performance of the transducer, but there is little research on this. Therefore, the influence of the coil excitation signal on transducer performance is investigated. The working principle and characteristics of the double-coil inductive displacement transducer are analyzed, and the circuit simulation model of the transducer is established. From the aspects of phase shift, linearity, and sensitivity, the effects of a sine signal, a triangle signal, and a pulse signal on the transducer are compared and analyzed. The results show that the average phase shift, linearity, and sensitivity of the sine signal were 11.53°, 1.61%, and 0.372 V/mm, respectively; the average phase shift, linearity and sensitivity of the triangular signal were 1.38°, 1.56%, and 0.300 V/mm, respectively; and the average phase shift, linearity, and sensitivity of the pulse signal were 0.73°, 1.95%, and 0.621 V/mm, respectively. It can be seen that the phase shift of a triangle signal and a pulse signal is smaller than that of a sine signal, which can result in better signal phase-locked processing. The linearity of the triangle signal is better than the sine signal, and the sensitivity of the pulse signal is better than that of the sine signal.
NURBS Interpolator with Minimum Feedrate Fluctuation Based on Two-Level Parameter Compensation
Authors: Nie Mingxing , Zhu Tao , Li Yue
Published: April 2023

Feedrate plays a crucial role in determining the machining quality, tool life, and machining time. Thus, this research aimed to improve the accuracy of NURBS interpolator systems by minimizing feedrate fluctuations during CNC machining. Previous studies have proposed various methods to minimize these fluctuations. However, these methods often require complex calculations and are not suitable for real-time and high-precision machining applications. Given the sensitivity of the curvature-sensitive region to feedrate variations, this paper proposed a two-level parameter compensation method to eliminate the feedrate fluctuation. First, in order to address federate fluctuations in non-curvature sensitive areas with low computational costs, we employed the first-level parameter compensation (FLPC) using the Taylor series expansion method. This compensation allows us to achieve a chord trajectory for the new interpolation point that matches the original arc trajectory. Second, even in curvature-sensitive areas, feedrate fluctuations can still occur because of truncation errors in the first-level parameter compensation. To address this, we employed the Secant-based method for second-level parameter compensation (SLPC), which does not require derivative calculations and can regulate feedrate fluctuation within the fluctuation tolerance. Finally, we applied the proposed method to the simulation of butterfly-shaped NURBS curves. These simulations demonstrated that our method achieved maximum feedrate fluctuation rates below 0.01% with an average computational time of 360 us, which is sufficient for high-precision and real-time machining. Additionally, our method outperformed four other feedrate fluctuation elimination methods, highlighting its feasibility and effectiveness.
Impoverishment Persistence in Hydropower Dam-Induced Resettled Communities: A Sociological Investigation on Livelihood and Food Security in Vietnam
Authors: Ty Pham Huu , Laura Baas , Phuong Tran Thi , Tan Nguyen Quang
Published: April 2023

The widespread development of hydropower dams has led to involuntary displacement, which has become a significant global issue. In Vietnam, around 70,000 households were displaced in 2020, causing uncertainty and social disruption. The aim of this study is to analyze the effects of resettlement on the livelihood and food security of displaced households, explore the underlying challenges and causes of these effects, and recommend policy implications for sustainable livelihood development and poverty alleviation. This study conducted a decade-long sociological examination of three displaced communities in Thua Thien Hue province, Vietnam. Our research reveals that resettled households are unable to regain their former standard of living due to the loss of cultivated land and restricted access to public property, which exacerbates food insecurity. Unemployment, illiteracy, and low income further perpetuate poverty. These findings highlight the deficiencies in current policies and planning approaches and call for implementing socially responsible resettlement processes guided by principles of equity. Addressing the inequalities arising from displacement and enabling affected communities to participate in growth is economically justified and morally imperative.