bluuebunny/crossref_metadata_2025 huggingface dataset:
تمرينات خاصة باستعمال أدوات تدريبية مساعدة في تطوير القوة المميزة بالسرعة للذراعين ومهارة الاستلام والتصويب بالقفز ثلاث نقاط للاعبي كرة السلة تحت 16سنة
Authors: Omar Khalil Alwan
Published: October 2024

يهدف البحث اعداد تمرينات خاصة باستعمال أدوات تدريبية مساعدة للاعـــــــبي كــرة السـلة تحت 16 سنة، معرفـة مدى تأثير التمرينات الخاصة باستعمال ادوات تدريبية مساعدة في تطوير القـوة المـميزة بالسرعة للذراعين للاعبي كــرة السـلة تحت 16 سنة، مـعرفة مدى تأثـير التمرينات الخاصة باستعـمال ادوات تدريبية مساعدة في تطوير مهارة الاستلام والتصويب بالقفز ثلاث نقاط للاعبي كــرة السـلة تحت 16 سنة، واستخدم الباحث المنهج التجريبي بتصميم المجموعتين المتكافئتين التجريبية والضابطة، اذ تم تحديد عينة البحث من لاعبي نادي نفط الشمال الرياضي تحت(16سنة)، وتم اجراء الاختبارات القبـلية والبعـدية لمجموعتي البـحث، وبعد خـضوع المجموعة التجريبية الى المتغير المستقل المعد من قبل الباحث والمجموعة الضابطة الى المنهج المتبع من قبل المدرب ومن ثم اجراء المقارنات بين نتائج الاختبارات لمجموعتي البحث في الاختبارات القبلية والبعدية باستخدام المعالجات الإحصائية استنتج الباحث أن القوة المميزة بالسرعة للذراعين للمجموعة التجريبية تطورت تطورا نسبياً عن المجموعة الضابطة وذلك بسبب تأثير التمرينات الخاصة باستعمال ادوات تدريبية مساعدة للاعبي كــرة السـلة، وأن مهارة الاستلام والتصويب بالقفز ثلاث نقاط تطورت نسبياً للمجموعة التجريبية أفضل من المجـموعة الضابطـة وذلك بسبب تأثير التــمرينات الخاصة باسـتـعمال الادوات التدريـبية المساعدة للاعـبي كــرة السـلة.
Gilyarevsky Rujero Sergeevich – founder of the information science
Authors: Tsvetkova V. A.
Published: October 2024

Rujero Sergeevich Gilyarevsky is a prominent Russian scholar, Doctor of Science in Philology, Professor, one of the founders of information science, wellknown library scientist, Honored Scientist of the Russian Federation, Chief Researcher, Head of Theoretical and Applied Problems Division of the All-Russian Institute for Scientific and Technical Information of the Russian Academy of Sciences. Rujero Gilyarevsky is among the most remarkable, vivid, and versatile individuals in the information and library community. He is characterized by creativity, scope of expertise and high competence.
Information market, educational and library environment in the contemporary digital setting: New trends and expectations (The annual paper delivered at the Eighth World Professional Forum “The Book. Culture. Education. Innovations” (“Gelendzhik-2024”))
Authors: Shrayberg Ya. L.
Published: October 2024

The author reviews and analyzes the status and prospects for the educational and library technologies and information market within the digitalized community. The increasing AI role, its advantages and drawbacks in education and publishing are discussed. The library behavioral models under the new circumstances are evaluated. The libraries’ role in education support and user services is emphasized.
Breast cancer statistics 2024
Authors: Giaquinto Angela N. , Sung Hyuna , Newman Lisa A. , Freedman Rachel A. , Smith Robert A. , Star Jessica , Jemal Ahmedin , Siegel Rebecca L.
Published: October 2024

This is the American Cancer Society's biennial update of statistics on breast cancer among women based on high‐quality incidence and mortality data from the National Cancer Institute and the Centers for Disease Control and Prevention. Breast cancer incidence continued an upward trend, rising by 1% annually during 2012–2021, largely confined to localized‐stage and hormone receptor‐positive disease. A steeper increase in women younger than 50 years (1.4% annually) versus 50 years and older (0.7%) overall was only significant among White women. Asian American/Pacific Islander women had the fastest increase in both age groups (2.7% and 2.5% per year, respectively); consequently, young Asian American/Pacific Islander women had the second lowest rate in 2000 (57.4 per 100,000) but the highest rate in 2021 (86.3 per 100,000) alongside White women (86.4 per 100,000), surpassing Black women (81.5 per 100,000). In contrast, the overall breast cancer death rate continuously declined during 1989–2022 by 44% overall, translating to 517,900 fewer breast cancer deaths during this time. However, not all women have experienced this progress; mortality remained unchanged since 1990 in American Indian/Alaska Native women, and Black women have 38% higher mortality than White women despite 5% lower incidence. Although the Black‐White disparity partly reflects more triple‐negative cancers, Black women have the lowest survival for every breast cancer subtype and stage except localized disease, with which they are 10% less likely to be diagnosed than White women (58% vs. 68%), highlighting disadvantages in social determinants of health. Progress against breast cancer could be accelerated by mitigating racial, ethnic, and social disparities through improved clinical trial representation and access to high‐quality screening and treatment.
Micro-plastics in soil environment: A review
Authors: Khan M R , Tarafder M A , Priti M S , Haque M A
Published: October 2024

Environmental scientists and other stakeholders have paid serious attention to soil pollution by microplastics in the last ten years. In soils, the microplastic particles act as a vector for the toxic persistent organic pollutants and potentially toxic metals that are easily sorbed by plants and enter the food chain. Microplastics are emerging as persistent terrestrial pollutants due to mismanagement and indiscriminate use. Microplastic contaminants affect the physicochemical characteristics of the soil as well as the feeding patterns of the soil biota. Sewage sludge, bio waste compost additions, plastic mulching, wastewater irrigation, landfill leachate, and air deposition are the causes of microplastics in soils. The amount of microplastics particles per kilogram of soil ranged from zero to thirteen thousand pieces. There are 523 times as many microplastic particles in the soil as there are in the ocean. Plant growth and seed germination are slowed down by the microplastic in the soil. Microplastics also affect the soil's enzymatic activities. The environmental sources of microplastic include plastic pellets, city dust, abrasion of road markings, tires, synthetic textiles, personal care products, and cosmetics. Human consumption through food can have a variety of harmful effects, including cytotoxicity, immunotoxicity, pulmonary toxicity, and reproductive toxicity. The current study describes the origins, distribution, and effects of microplastics on plants, soil biota, and human health in the soil environment.
Bangladesh J. Nuclear Agric, 38(1): 1-19, 2024
Relationships between flowering behavior and seed yield in mungbean mutants
Authors: Mondal M M A , Sen A
Published: October 2024

Flower is the prime sink that produces pods and yield depends on numbers and size of pods in legume crops. Flowering behavior affects the synchrony of pod maturity, which is crucial for efficient harvesing that regulates the yield. Thus, it is essential to study the relationship between flowering behavior and pod yield in mungbean mutants. This experiment was conducted using a Randomized Complete Block Design (RCBD) with three replications at the BINA headquarters physiology pot yard. The aim was to assess the relationship between flowering patterns, flowering duration, and seed yield in ten mungbean mutants/varieties. Results indicated that high-yielding mutant (MM-1) produced more opened flowers (38.0 flowers per plant) and had a longer flowering duration (12 days) compared to low-yielding varieties/mutants (13.1-18.0 flowers per plant and 7-9 days). Additionally, high-yielding varieties exhibited higher flower production rates over time, with peak flower production occurring within 5-8 days, while low-yielding varieties peaked within 3-4 days. High-yielding mungbean mutants/varieties exhibit lower pod set and reproductive efficiency (48.7-55.0%) compared to low-yielding ones (63.2-70.9%), indicating potential for yield improvement in high-yielding genotypes through enhanced pod set. Pod number, a key yield parameter, has a significant positive correlation with flower number (r = 0.56**), while pod number is also dependent on flowers per plant (r = 0.68**). Additionally, seed yield shows a strong positive relationship with the harvest index (r = 0.62**). These findings suggest that future breeding efforts should focus on synchronizing flower production to increase mungbean yield.
Bangladesh J. Nuclear Agric, 38(1): 111-117, 2024
Variation in the Growth and Popping Indicators of Argentina-derived Popcorns in Vietnam
Authors: Nguyen Loc Van , Aracena Guillermo Eugenio , Le Luong Thanh , Rumanzi Mbaraka Saidi , Nguyen Viet Long
Published: October 2024

The development of popcorn genotypes that are high-yielding and with high popping abilities is fundamental to the expansion of popcorn production. In this study, a total of 23 newly developed full-sib FS3 lines of popcorn were evaluated under upland field conditions in the spring cropping season in 2021. The plant genotypes were evaluated for growth duration, plant height, number of leaves, ears/plant, ear length, ear diameter; the number of kernels, seeds per row, 1000-seeds weight, seed yield, and the proportion of floury endosperm, and popping indicator. Our results showed that the growth duration of the popcorn lines ranged from 96 - 121 days, plants were between 103.7 - 200.6 cm in height, and the number of leaves ranged between 12.92-18.13. The number of ears/plant, ear length, ear diameter, and number of kernel rows and seeds per row were 0.3-1.3, 9.6 - 20 cm, 2.3 - 4.2 cm, 7.33 -13.6 and 6.2 - 31.9, respectively. The popcorn lines were diverse in grain color and classified into white, yellow, black, pink, white yellow, and white pink. The grain yield varied from 2.13 - 64.58 quintals/ha, in which NO-11 had the highest yield of 64.58 quintals/ha. Popping indicators varied among genotypes and were negatively and positively correlated with 1000-seed weight and proportion of floury endosperm, respectively. In this study, we identified some genetic resources, such as NO-2, NO-12, NO-14, NO-20, and NO-22, which had the best popping ability. These resources will provide insights into the mechanisms of popping ability and important materials for breeding programs.
J Bangladesh Agril Univ 22(3): 285-294, 2024
Ultrasonographic Udder Monitoring and Disease diagnosis in She-goat
Authors: Safawat Mst Sanjida , Naher Samsun , Sakib Mahmudul Hasan , Hassan Nahid , Rahim Md Abdur , Juyena Nasrin Sultana
Published: October 2024

Ultrasonography is an effective, non-invasive technique for regular monitoring of udder health to ensure the best quality of milk. The study was conducted to measure the diameter of gland cistern before and after milking and evaluate the effect of breeds, age, and seasons on udder diseases in she-goats. The diameter was measured through ultrasonography before and after milking and retrospective data (breed, age, season) were collected from the Veterinary Teaching Hospital, Mymensingh. Variation in gland cistern diameter (mm) was significant (p<0.05), meaning that the gland cistern’s diameter changed more before and after milking. Results also revealed an insignificant (p>0.05) difference between the left and right quarter gland cistern’s diameter independent of time. This study showed that the occurrence of udder diseases was higher in the Black Bengal breed (82%) compared to Jamuna Pari (18%). Mastitis mostly occurred at the age of between 2 to 3 years old (25.7%) and at the winter season (39.8%). All age groups had an affected incidence of 1.8% for udder wounds and showed higher occurrence during the summer season (5.3%). Furthermore, this study indicated that udder edema affection is more common in the group of goats younger than two years old and during the winter season (3.5%) This study concludes that normal physiological structures i.e. udder parenchyma, gland cistern and different pathological conditions such as mastitis, fibrosis can be easily monitored through transcutaneous ultrasonography.
J Bangladesh Agril Univ 22(3): 396-403, 2024
Etirkālat talaimuṟaiyiṉariṭam Tamiḻc caiva camaya uṇarvaip perukkuvataṟkāṉa vittum viḷaivu [Strategies and methods to effectively root Saivism among our future generation]
Authors: Dharmalingam Agalya , S.Kandasamy Silllalee
Published: October 2024

The term ‘Samayam’ in Tamil language refers to a process which nurtures souls into maturity and differentiates humans from animals. It is also said to elevate an average one to become a Godlike being. Here, Godliness is that one becomes highly aware of his/her self and the cosmological realm; realizing one’s ultimate Saviour Sivam; becoming an invincible compassionate being in this world. However, the contemporary world has brought severe abatement upon a pious lifestyle. The Saivite exponents who have foreseen these social symptoms have left us with insights and possible ways to instill Saivism to the future generations. Furthermore, Periyapuranam greatly exhibits how a child can be gradually introduced to Saivism commencing from even before the day of conception and continuing throughout life. The aim of this study is to ascertain established methods used in Periyapuranam in instilling Saivism into the future generation which are also applicable in present times.
IMPACT OF DISTRESS LEVEL IN RIGHT ISSUE PRICING AND SHARE PERFORMANCE
Authors:   Petra Christian University, Sadjiarto Arja , Natadjaja Michela ,   Petra Christian University, Sutikno Fiona ,   Petra Christian University
Published: October 2024

Research Purposes. This research aims to observe the influence of distress level and leverage on right issue subscription pricing since previous studies mainly focused on the subscription price’s impact rather than its determinants. For further analysis, this research also evaluates the subscription price, distress level, and leverage’s influence on share performance. Research Methods. Data analysis technique employs multiple linear regression with Ordinary Least Square (OLS) method, utilizing data from 70 public listed companies which are recorded to have published right issue in Indonesia Stock Exchange during 2004 to 2022. Research Results and Findings. This research discovered that distress level influences the right issue subscription price, and the right issue subscription price significantly influences share performance. Thus, the result of this research may be utilized by companies which are distressed, high-leveraged, or in possession of the characteristics in line with the control variables to consider the most ideal subscription price as well as its implications to the companies’ share performance.
Numerical investigation of the scavenge pump performance in an aircraft engine lubrication system under pure oil and oil-air two-phase medium
Authors:   Northwestern Polytechnical University, Wu Nan , Hu Jianping ,   Northwestern Polytechnical University, Lyu YaGuo ,   Northwestern Polytechnical University, Tan Yi ,   Northwestern Polytechnical University, Liu Zhenxia ,   Northwestern Polytechnical University
Published: October 2024

As an important component of the aircraft engine lubrication system, the scavenge pump plays a crucial role in the proper circulation of the lubrication system by its suction capability for the oil-air mixture. Taking a certain aircraft engine two-stage scavenge pump as the research object, the internal two-phase flow of oil and air within the pump was simulated based on the volume of fluid model. The paper mainly analyzed the impact of rotation speed and oil-air ratio on flow rate and volume efficiency, which serve as evaluation indicators for the suction capability of the scavenge pump. The operating performance of the pump was compared between the stage 1 and stage 2. Additionally, a comparison was conducted between numerical results and experimental results, indicating a good agreement in the lubricating oil flow rate. The analysis investigated the reasons for the discrepancies in the volumetric efficiency of the pump between the numerical solutions and experimental results, providing a more scientifically grounded reference for the testing of pump performance and subsequent optimization design.
Power limits of wind turbines in the energy transition scenario
Authors:   DICAM, Battisti Lorenzo
Published: October 2024

The future huge requirement of ’green’ power to implement the countries’ energy transition programs, foresees a consistent contribution of wind turbines in electric energy generation. Beside of evaluation of the wind potential, it is technically essential to understand the marginal gain of electrical power potentially deliverable by the single turbine unit and hence the maximum power attainable under the expected technology development. In this study the concept of power is analysed through the compositional factors of the specific work, the efficiency, and the mass flow. This approach allows us to deduce the areas of potential improvement at the current technology level. The giantism of the turbines is hampered by several factors, like as the aerodynamic efficiency, the structural resistance of some components, and the availability of the necessary wind energy fluxes, that set an upper limit to the deliverable power. This limit plays a fundamental role in determining the future need of onshore and offshore locations to achieve the massive power levels connected to energy transition goals.
Makna Frasa “ὁ ἀμνὸς τοῦ Θεοῦ” dalam Yohanes 1:29
Authors: Lucas Tjong Andrew , Binar Sri
Published: October 2024

One of the theological titles addressed to Jesus Christ is the Lamb of God. This title can be found in John 1:29 and 1:36 which is the title of John the Baptist to Jesus,and is not found in the Synoptic Gospels. A number of scholars have attempted to interpret the phrase Lamb of God both in a literal and figurative sense and there are a number of different meanings. In this paper, the author seeks the meaning of the phrase Lamb of God in John 1:29 using the exegetical method. The exegetical method used three analyses: contextual analysis, historical, and grammatical and literal analysis. From the analysis carried out, it was found that the meaning of the phrase Lamb of God which is directed at Jesus explains the work of Jesus' salvation through His death on the cross which brought redemption and remission of sins for humans by taking Jewish background: the Passover lamb, and the divine qualities possessed by Christ as the Lamb of God.
“Negasi dan Harmoni Pesimisme:” Studi Eksposisi Pengkhotbah 1:12-18 dan Manfaat Hikmat Amsal
Authors: Manusiwa Junette Hendrika Martha , Sualang Farel Yosua
Published: October 2024

One of the wisdom literature books that is frequently quoted, though often interpreted out of context, is the book of Ecclesiastes, which remains relevant to the life of believers. However, there are still differences in interpretation and limitations in historical cultural analysis, as well as varying approaches to textual focus and accountability in understanding life. This article aims to bridge the contradictory aspects of the vanity of wisdom in Ecclesiastes 1:12-18 and its harmony with the benefits of wisdom in the book of Proverbs. The methodology used in this research is wisdom literature hermeneutics, which includes an in-depth textual analysis and historical analysis of the text of Ecclesiastes 1:12-18. This approach provides a bridging understanding that allows for recognizing the contradictions (negation) and harmony in the books of Ecclesiastes and Proverbs, as well as their theological and practical implications for readers. Despite the variety of interpretations, integrating these elements offers a comprehensive view of wisdom and provides a critical reflection both scientifically and practically for real-life application through a relevant, proportional, and balanced theological interpretation in understanding hebel and hokmah. Thus, the awareness of the limitations of human wisdom should be oriented towards complete dependence on the fear of the Lord.
Merangkul Kembali yang Terasing: "Strategi Praktis Gereja dalam memanggil kembali Jemaat yang Jarang Beribadah"
Authors: Simanjuntak Joksan , Tambun Roy Haries Ifraldo
Published: October 2024

The increasing number of congregants who rarely attend worship has become a serious challenge for many churches in maintaining spiritual engagement and community commitment. This study aims to identify the factors that cause congregants to distance themselves from worship activities and to explore strategies that churches can implement to reengage alienated members. Using a qualitative approach, this research involved in-depth interviews with 8 selected congregants who had demonstrated inactivity in worship, focusing on a case study at Church HKBP Pardamean. The interview findings revealed several key factors contributing to the lack of attendance, including the rising demands of modern life, individualism influenced by technology, and disappointment with internal church aspects. Based on these findings, the study recommends practical strategies for churches, such as personalized approaches and strengthening the sense of belonging among congregants. This article aims to provide guidance for churches in addressing the issue of congregational disengagement and developing a more inclusive pastoral approach.
Kebangkitan sebagai Manifestasi Kuasa Allah: Pendekatan Historis-Kritis terhadap Matius 22:23-33 dalam Kontras Keyakinan Yudaisme Reformasi dan Kristen
Authors: Tambun Roy Haries Ifraldo
Published: October 2024

This study aims to analyze the concept of resurrection in Christian and Reform Judaism beliefs, emphasizing that resurrection is a manifestation of God's power over life and death. The study contrasts the Christian belief, which regards resurrection as an integral part of faith, with the Reform Judaism view, which generally rejects resurrection as a literal fact. Using the Historical-Critical interpretation method and a qualitative approach, this research analyzes Matthew 22:23-33 through inner-text and inter-text approaches to reveal its theological meaning and context. The findings show that resurrection is not merely a theological doctrine in the Christian faith, but also serves as evidence of God's power over life and death, aligning with the belief that those who die in faith in God will be resurrected. In conclusion, this research makes a significant contribution to the theological understanding of resurrection, highlights its distinction from Reform Judaism beliefs, and reinforces the role of resurrection as a key element in Christian faith.
Research progress on blood compatibility of hemoperfusion adsorbent materials
Authors: Zhang Liangqing , Liu Guohao , Xia Qingping , Deng Li
Published: October 2024

This comprehensive review examines the latest developments in improving the blood compatibility of hemoperfusion adsorbents. By leveraging advanced coating and modification techniques, including albumin-collodion, cellulose, hydrogel, and heparin coatings, notable enhancements in blood compatibility have been achieved across diverse adsorbent types, such as carbon-based, resin-based, and polysaccharide-based materials. Despite promising laboratory results, the intricate manufacturing processes and elevated costs present significant challenges for broad clinical application. Therefore, future endeavors should focus on cost-benefit analysis, large-scale production strategies, in-depth exploration of blood-material interactions, and innovative technologies to propel the development of safer and more effective blood purification therapies.
Drivers of cardiovascular disease in metabolic dysfunction-associated steatotic liver disease: the threats of oxidative stress
Authors: Minetti Erika T. , Hamburg Naomi M. , Matsui Reiko
Published: October 2024

Non-alcoholic fatty liver disease (NAFLD), now known as metabolic-associated steatotic liver disease (MASLD), is the most common liver disease worldwide, with a prevalence of 38%. In these patients, cardiovascular disease (CVD) is the number one cause of mortality rather than liver disease. Liver abnormalities per se due to MASLD contribute to risk factors such as dyslipidemia and obesity and increase CVD incidents. In this review we discuss hepatic pathophysiological changes the liver of MASLD leading to cardiovascular risks, including liver sinusoidal endothelial cells, insulin resistance, and oxidative stress with a focus on glutathione metabolism and function. In an era where there is an increasingly robust recognition of what causes CVD, such as the factors included by the American Heart Association in the recently developed PREVENT equation, the inclusion of liver disease may open doors to how we approach treatment for MASLD patients who are at risk of CVD.
Assessing peer teamwork competence: adapting and validating the comprehensive assessment of team member effectiveness–short in university students
Authors: Garalde Alain , Solabarrieta Josu , Urquijo Itziar , Ortiz de Anda-Martín Irati
Published: October 2024

Introduction

The Bologna Plan has promoted competency-based training in European higher education, which requires developing accurate, appropriate assessment tools for its measurement and evaluation.

Method

This study adapted and validated the Comprehensive Assessment of Team Member Effectiveness questionnaire on teamwork competence in a university population in the Basque Country, Spain. The questionnaire was first back-translated and adapted by two experts in educational research and then completed by 642 students pursuing baccalaureate degrees in primary education and physical activity and sport sciences at the University of Deusto [215 women, 426 men; ages: 17–25 years (M = 21.5; SD = 1.3)].

Results and discussion

A confirmatory factor analysis yielded adequate goodness-of-fit indices, confirming the validity of the instrument’s measurement model and indicating that it may be an appropriate tool for assessing teamwork competence among peers for university students in the Basque Country.
Course of pregnancy and 10-year observation of twins diagnosed with GCK-MODY in the neonatal period: a case report
Authors: Katra Barbara , Szopa Magdalena
Published: October 2024

Monogenic diabetes accounts for 5% of all incidence of hyperglycemia and Maturity Onset Diabetes of the Young (MODY) is the most common form. In GCK-MODY, one of the most common forms of MODY, hyperglycemia is caused by a mutation of a gene responsible for coding glucokinase. At the clinical level, this condition presents as persistent, moderate and asymptomatic elevated fasting glucose levels and has a relatively low incidence of micro and macro-vascular complications. In general, the treatment of choice is to follow and maintain a healthy lifestyle. The incidence of GCK-MODY during pregnancy is 2% on average (0-6%). In this report, we introduce a case of a woman diagnosed with GCK-MODY during the pregnancy with twins, a boy and a girl, diagnosed with GCK-MODY after birth. We discuss the course of pregnancy, the need for access to fast and uncomplicated genetic diagnostics in utero, and the impact of the MODY diagnosis on the life of the mother and that of her children. In our case, the diagnosis of GCK-MODY was associated with a feeling of relief, after years of uncertainty, and helped to introduce more appropriate eating behaviors and lifestyle changes for both the mother and her children.
Integrated PacBio SMRT and Illumina sequencing uncovers transcriptional and physiological responses to drought stress in whole-plant Nitraria tangutorum
Authors: Wei Meiying , Wang Bo , Li Chaoqun , Li Xiaolan , He Cai , Li Yi
Published: October 2024

Introduction

Nitraria tangutorum Bobr., a prominent xerophytic shrub, exhibits remarkable adaptability to harsh environment and plays a significant part in preventing desertification in northwest China owing to its exceptional drought and salinity tolerance.

Methods

To investigate the drought-resistant mechanism underlying N. tangutorum, we treated 8-week-old seedlings with polyethylene glycol (PEG)-6000 (20%, m/m) to induce drought stress. 27 samples from different tissues (leaves, roots and stems) of N. tangutorum at 0, 6 and 24 h after drought stress treatment were sequenced using PacBio single-molecule real-time (SMRT) sequencing and Illumina RNA sequencing to obtain a comprehensive transcriptome.

Results

The PacBio SMRT sequencing generated 44,829 non-redundant transcripts and provided valuable reference gene information. In leaves, roots and stems, we identified 1162, 2024 and 232 differentially expressed genes (DEGs), respectively. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that plant hormone signaling and mitogen-activated protein kinase (MAPK) cascade played a pivotal role in transmitting stress signals throughout the whole N. tangutorum plant following drought stress. The interconversion of starch and sucrose, as well as the biosynthesis of amino acid and lignin, may represent adaptive strategies employed by N. tangutorum to effectively cope with drought. Transcription factor analysis showed that AP2/ERF-ERF, WRKY, bHLH, NAC and MYB families were mainly involved in the regulation of drought response genes. Furthermore, eight physiological indexes, including content of proline, hydrogen peroxide (H2O2), malondialdehyde (MDA), total amino acid and soluble sugar, and activities of three antioxidant enzymes were all investigate after PEG treatment, elucidating the drought tolerance mechanism from physiological perspective. The weighted gene co-expression network analysis (WGCNA) identified several hub genes serve as key regulator in response to drought through hormone participation, ROS cleavage, glycolysis, TF regulation in N. tangutorum.

Discussion

These findings enlarge genomic resources and facilitate research in the discovery of novel genes research in N. tangutorum, thereby establishing a foundation for investigating the drought resistance mechanism of xerophyte.
Impact of seawater warming and nutrient deprivation on the physiology and energy metabolism of corals
Authors: Pupier Chloé A. , Grover Renaud , Rottier Cécile , Ferrier-Pagès Christine
Published: October 2024

Seawater temperature and the availability of dissolved inorganic nutrients (DINut) have a major influence on the stability of the symbiosis between corals and Symbiodiniaceae. In particular, seawater warming or DINut depletion can lead to coral bleaching, the loss of Symbiodiniaceae from coral tissue. However, the combined effects of heat stress and DINut deficiency on the coral energy metabolism are still understudied. Here, we investigated the physiological and energetic responses of the octocoral Heteroxenia fuscescens and the hexacoral Stylophora pistillata exposed to two levels of inorganic nutrients in seawater (control, depleted) and two temperatures, 25°C (control) and 30°C (high temperature), in a crossed factorial design. Our results show that thermal and DINut stress both decreased the photosynthesis to respiration ratio of the two species and induced bleaching in H. fuscescens. While nutrient deprivation had little effect on the corals’ energy metabolism, heat stress led to higher concentrations of macromolecules such as carbohydrates and lipids, as well as anaerobic metabolism, and decreased ATP production in H. fuscescens. Given that the intensity and frequency of marine heatwaves will significantly increase in the future, there is an urgent need to investigate the processes by which corals can overcome starvation.
Applications of different forms of nitrogen fertilizers affect soil bacterial community but not core ARGs profile
Authors: You Ruiqiang , Yu Yang , Shen Min , Zhang Yanzhou , Hong Jian , Kang Yijun
Published: October 2024

The objective of this study was to investigate the impact of various chemical nitrogen fertilizers on the profile of antibiotic resistance genes (ARGs) in soil. A microcosm experiment was conducted with four treatments, including CK (control with no nitrogen), AN (ammonium nitrogen), NN (nitrate nitrogen), and ON (urea nitrogen), and the abundance of ARGs was assessed over a 30-day period using a metagenomic sequencing approach. The levels of core ARGs varied between 0.16 and 0.22 copies per cell across different treatments over time. The abundance of core ARGs in the ON treatment closely resembled that of the CK treatment, suggesting that environmentally friendly nitrogen fertilizers, particularly those in controlled release formulations, may be preferable. The core ARG abundance in the AN and NN treatments exhibited noticeable fluctuations over time. Overall, chemical nitrogen fertilizers had minimal effects on the core ARG profile as determined by principal component analysis and clustering analyses. Conversely, distinct and significant changes in bacterial communities were observed with the use of different nitrogen fertilizers. However, the influence of nitrogen fertilizers on the core ARGs is limited due to the unaffected potential bacterial hosts. Nitrogen-cycling-related genes (NCRGs), such as those involved in nitrogen-fixing (nifK, nifD, nifH) and denitrification (narG, napA, nirK, norB, nosZ) processes, exhibit a positive correlation with ARGs (rosA, mexF, bacA, vanS), indicating a potential risk of ARG proliferation during intense denitrification activities. This study indicates that the application of chemical nitrogen has a minimal effect on the abundance of ARGs in soil, thereby alleviating concerns regarding the potential accumulation of ARGs due to the use of chemical nitrogen fertilizers.
1,3,4-oxadiazoles as inhibitors of the atypical member of the BET family bromodomain factor 3 from Trypanosoma cruzi (TcBDF3)
Authors: Alonso Victoria L. , Escalante Andrea M. , Rodríguez Araya Elvio , Frattini Gianfranco , Tavernelli Luis E. , Moreno Diego M. , Furlan Ricardo L. E. , Serra Esteban
Published: October 2024

Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, affects millions globally, with increasing urban cases outside of Latin America. Treatment is based on two compounds, namely, benznidazole (BZ) and nifurtimox, but chronic cases pose several challenges. Targeting lysine acetylation, particularly bromodomain-containing proteins, shows promise as a novel antiparasitic target. Our research focuses on TcBDF3, a cytoplasmic protein, which is crucial for parasite differentiation that recognizes acetylated alpha-tubulin. In our previous study, A1B4 was identified as a high-affinity binder of TcBDF3, showing significant trypanocidal activity with low host toxicity in vitro. In this report, the binding of TcBDF3 to A1B4 was validated using differential scanning fluorescence, fluorescence polarization, and molecular modeling, confirming its specific interaction. Additionally, two new 1,3,4-oxadiazoles derived from A1B4 were identified, which exhibited improved trypanocide activity and cytotoxicity profiles. Furthermore, TcBDF3 was classified for the first time as an atypical divergent member of the bromodomain extraterminal family found in protists and plants. These results make TcBDF3 a unique target due to its localization and known functions not shared with higher eukaryotes, which holds promise for Chagas disease treatment.
Noise-induced cochlear synaptopathy in C57BL/6 N mice as a function of trauma strength: ribbons are more vulnerable than postsynapses
Authors: Blum Kerstin , Schepsky Pauline , Derleder Philip , Schätzle Philipp , Nasri Fahmi , Fischer Philipp , Engel Jutta , Kurt Simone
Published: October 2024

Noise-induced cochlear synaptopathy is characterized by irreversible loss of synapses between inner hair cells (IHCs) and spiral ganglion neurons (SGNs) despite normal hearing thresholds. We analyzed hearing performance and cochlear structure in C57BL/6 N mice exposed to 100, 106, or 112 dB SPL broadband noise (8–16 kHz) for 2 h. Auditory brainstem responses (ABRs) were assessed before, directly after, and up to 28 days post-trauma. Finally, the number, size, and pairing of IHC presynaptic (CtBP2-positive) ribbons and postsynaptic AMPA receptor scaffold (Homer1-positive) clusters were analyzed along the cochlea. Four weeks after the 100 dB SPL trauma, a permanent threshold shift (PTS) was observed at 45 kHz, which after the higher traumata extended toward middle to low frequencies. Loss in ABR wave I amplitudes scaled with trauma strength indicating loss of functional IHC synaptic connections. Latencies of wave I mostly increased with trauma strength. No trauma-related OHC loss was found. The number of synaptic pairs was reduced in the midbasal and basal cochlear region in all trauma conditions, with ribbon loss amounting up to 46% of control. Ribbons surviving the trauma were paired, whereas 4–6 unpaired postsynapses/IHC were found in the medial, midbasal, and basal regions irrespective of trauma strength, contrasting findings in CBA/CaJ mice. Our data confirm the susceptibility of ribbon synapses and ABR wave I amplitudes to a noise trauma of 100 dB SPL or larger. Notably, peripheral dendrites bearing IHC postsynapses were less vulnerable than presynaptic ribbons in C57BL/6 N mice.
Variants loci and phenotype correlation of TRIM8-related neuro-renal syndrome: three cases reports and literature review
Authors: Lv Qiu , Niu Yue , Xu Zhao , Qin Jiong , Yang Zhixian
Published: October 2024

Background

TRIM8-related neuro-renal syndrome (NRS), caused by pathogenic variants of the TRIM8 gene, is characterized by epilepsy, developmental delay (DD) and renal disorders. The severity of the neurological effects as well as the presence of renal disorders is variable among patients. Here, we report three additional patients with clinical features compatible with NRS and summarize the association between the variants’ loci and phenotype of TIRM8-related NRS.

Methods

A retrospective analysis was conducted for three Chinese children with NRS due to TRIM8 variants identified through whole-exome sequencing (WES). Previous reports of patients with TRIM8-related NRS were reviewed systematically. Demographic and clinical data were collected from these patients

Results

Two de novoTRIM8 truncating variants in three NRS patients were identified in our study, including c.1327_c.1328delCCinsTG (p. Arg443*) and c.1375C>T (p.Gln459*). Our three patients all exhibited drug-resistant epilepsy and early-onset DD, and two of whom developed electrical status epilepticus during sleep (ESES). Brain magnetic resonance imaging (MRI) showed periventricular leukomalacia in one patient and normal in the other two. All three patients demonstrated nephrotic range proteinuria (NRP) or nephrotic syndrome (NS) with normal renal function during follow-up. There was a total of 27 patients with TRIM8-related NRS have been identified to date. The most common clinical features are renal diseases (89%), DD (89%), followed by epilepsy (78%). 67% of patients eventually progressed to end-stage renal disease (ESRD). Focal seizure was the most frequent seizure type (57%). 52% of patients presented drug-resistant epilepsy. 64% of patients exhibited non-specific brain MRI abnormalities. Brain atrophy was the most common change (50%). Two patients with TRIM8 variants closer to the N-terminal had neurological diseases without renal damage. Five patients with TRIM8 variants closer to the C-terminal had no severe neurological diseases. Seven patients had Gln459* variant which is the most common variant (7/27, 25.9%). The severity of the renal and neurological damage of the seven patients was variable.

Conclusion

This study expands the number of individuals with confirmed NRS due to pathogenic variants in TRIM8. Neurological and renal phenotype with the same variant locus differed in their severity. Further research is needed to explore the relationship between genotype and phenotype of TRIM8 variants.
Pre- and post-therapy functional MRI connectivity in severe acute brain injury with suppression of consciousness: a comparative analysis to epilepsy features
Authors: Cediel Emilio G. , Duran Erika A. , Laux Jeffrey , Reuther William , Leggio Olivia , Robinson Belfin , Boerwinkle Varina L.
Published: October 2024

Severe acute brain injury (SABI) with suppressed consciousness is a major societal burden, with early prognosis being crucial for life-and-death treatment decisions. Resting-state functional MRI (rs-fMRI) is promising for prognosis and identifying epileptogenic activity in SABI. While established for SABI prognosis and seizure networks (SzNET) identification in epilepsy, the rs-fMRI use for SzNET detection in SABI is limited. This study compared evolution of SzNET and resting-state networks (RSN) pre-to-post treatment in SABI and epilepsy, hypothesizing that changes would align with clinical evolution. Therapies included epilepsy surgery for the epilepsy group and antiseizure medication for the SABI group. Independent component analysis (ICA) was used to identify SzNET and RSNs in all rs-fMRI. High-frequency BOLD (HF-BOLD), an ICA power spectrum-based index, quantified RSN and SzNET changes by the patient. Confidence intervals measured HF-BOLD changes pre-to-post-therapy. Baseline HF-BOLD and HF-BOLD changes were compared using linear-mixed models and interaction tests. Five SABI and ten epilepsy patients were included. SzNET were identified in all SABI's pre-therapy rs-fMRI. The clinical changes in SABI and epilepsy were consistent with rs-fMRI findings across groups. HF-BOLD reduced in the epilepsy group RSN post-therapy (−0.78, 95% CI −3.42 to −0.33), but the evidence was insufficient to determine an HF-BOLD reduction in SABI patients or SzNET. The HF-BOLD change trend in pre-to-post epilepsy surgery scans paralleled the clinical improvement, suggesting that the power spectrum may quantify the degree of abnormality on ICA-derived networks. Despite limitations such as small sample sizes, this exploratory study provides valuable insights into network dysfunction in SABI and epilepsy.
Revising cancer incidence in a Central European country: a Hungarian nationwide study between 2011–2019 based on a health insurance fund database
Authors: Kiss Zoltán , Szabó Tamás G. , Polgár Csaba , Horváth Zsolt , Nagy Péter , Fábián Ibolya , Kovács Valéria , Surján György , Barcza Zsófia , Kenessey István , Wéber András , Wittmann István , Molnár Gergő Attila , Gyöngyösi Eszter , Benedek Angéla , Karamousouli Eugenia , Abonyi-Tóth Zsolt , Bertókné Tamás Renáta , Fürtős Diána Viktória , Bogos Krisztina , Moldvay Judit , Gálffy Gabriella , Tamási Lilla , Müller Veronika , Krasznai Zoárd Tibor , Ostoros Gyula , Pápai-Székely Zsolt , Maráz Anikó , Branyiczkiné Géczy Gabriella , Hilbert Lászlóné , Tamás Berki Láśzló , Rokszin György , Vokó Zoltán
Published: October 2024

Background

The nationwide HUN-CANCER EPI study examined cancer incidence and mortality rates in Hungary from 2011 to 2019.

Methods

Using data from the National Health Insurance Fund (NHIF) and Hungarian Central Statistical Office (HCSO), our retrospective study analyzed newly diagnosed malignancies between Jan 1, 2011, and Dec 31, 2019. Age-standardized incidence and mortality rates were calculated for all and for different tumor types using both the 1976 and 2013 European Standard Populations (ESP).

Findings

The number of newly diagnosed cancer cases decreased from 60,554 to 56,675 between 2011–2019. Age-standardized incidence rates were much lower in 2018, than previously estimated (475.5 vs. 580.5/100,000 person-years [PYs] in males and 383.6 vs. 438.5/100,000 PYs in females; ESP 1976). All-site cancer incidence showed a mean annual decrease of 1.9% (95% CI: 2.4%-1.4%) in men and 1.0% (95% CI:1.42%-0.66%) in women, parallel to mortality trends (-1.6% in males and -0.6% in females; ESP 2013). In 2018, the highest age-standardized incidence rates were found for lung (88.3), colorectal (82.2), and prostate cancer (62.3) in men, and breast (104.6), lung (47.7), and colorectal cancer (45.8) in women. The most significant decreases in incidence rates were observed for stomach (4.7%), laryngeal (4.4%), and gallbladder cancers (3.5%), with parallel decreases in mortality rates (3.9%, 2.7% and 3.2%, respectively).

Interpretation

We found a lower incidence of newly diagnosed cancer cases for Hungary compared to previous estimates, and decreasing trends in cancer incidence and mortality, in line with global findings and the declining prevalence of smoking.
Metabolic modulation of melanoma enhances the therapeutic potential of immune checkpoint inhibitors
Authors: Gurel Zafer , Luy Michael S. , Luo Qianyun , Arp Nicholas L. , Erbe Amy K. , Kesarwala Aparna H. , Fan Jing , Kimple Randall J.
Published: October 2024

Introduction

Lactate is a pivotal molecule with diverse functions in the metabolic reprogramming of cancer cells. Beyond its role in metabolism, lactate exerts a modulatory effect within the tumor microenvironment; it is utilized by stromal cells and has been implicated in the suppression of the immune response against the tumor.

Methods

Using in vitro assays (including flow cytometry, live-cell imaging and metabolic analyses), the impact of lactate dehydrogenase inhibitors (LDHIs) on melanoma cells were assessed. The therapeutic potential of LDHIs with immune checkpoint inhibitors (ICIs) were tested in vivo in murine models of melanoma tumors.

Results

A potent anti-proliferative effect (via both cell cycle alterations and enhanced apoptosis) of LDHIs, Oxamate (Oxa) and methyl 1-hydroxy-6-phenyl-4-(trifluoromethyl)-1H-indole-2-carboxylate (NHI-2), was found upon treatment of melanoma cell lines. Using a combination of Oxa and NHI-2, a synergistic effect to inhibit proliferation, glycolysis, and ATP production was observed. Metabolic analysis revealed significant alteration in glycolysis and oxidative phosphorylation, while metabolite profiling emphasized consequential effects on lactate metabolism and induced energy depletion by LDHIs. Detection of increased RANTES and MCP-1, with Oxa and NHI-2 treatment, prompted the consideration of combining LDHIs with ICIs. In vivo studies using a murine B78 melanoma tumor model revealed a significant improvement in treatment efficacy when LDHIs were combined with ICIs.

Conclusions

These findings propose the potential of targeting lactate metabolism to enhance the efficacy of ICI treatments in patients with melanoma.
Stage migration and survival outcomes in patients with cervical cancer at Stage IIIC according to the 2018 FIGO staging system: a systematic review and meta-analysis
Authors: Han Ling , Chen Yali , Zheng Ai , Tan Xin , Chen Hengxi
Published: October 2024

Objective

To summarize stage migration and survival outcomes in patients with cervical cancer at Stage IIIC according to the 2018 FIGO staging system, and to investigate prognostic factors influencing Stage IIIC1.

Methods

PubMed, Embase, Cochrane Central Register of Controlled Trials (CENTRAL), International Clinical Trials Registry Platform (ICTRP), and Clinical Trials.gov were searched from inception to February 6, 2024. The analysis was conducted using STATA 16.0.

Results

A total of 25 studies with 82954 cervical cancer patients were included in the analysis. The migration rates to FIGO 2018 Stage IIIC ranged from 18% to 37% for early-stage tumors (Stage IB to IIA) in FIGO 2009, and from 32% to 52% for advanced stage tumors (Stage IIB to IIIB). The overall survival (OS) for Stage IIIC1 is poorer compared to Stage IB1 (HR 0.53, 95% CI 0.35-0.80, p=0.003) and Stage IB2 (HR 0.61, 95% CI 0.43-0.85, p=0.004). It is comparable to Stage IB3, yet it shows better survival outcomes than Stages IIB (HR 2.91, 95% CI 1.01-8.39, p=0.047), IIIA (HR 1.96, 95% CI 1.78-2.17, p=0.000), and IIIB (HR 1.56, 95% CI 1.04-2.35, p=0.031). Tumors size ≥4cm (HR 1.45, 95% CI 1.10-1.92, p=0.00), metastatic lymph node ≥ 3 (HR 2.21, 95% CI 1.56-3.15, p=0.000) and T stage are prognostic factors for OS of Stage IIIC1.

Conclusions

The migration rates to FIGO 2018 Stage IIIC varied between 18% and 52% for patients initially classified under FIGO 2009 Stages IB1 to IIIB. The FIGO 2018 staging system underscores the pivotal role of lymph node metastasis in predicting prognosis and provides valuable insights into the distinct prognostic implications associated with different stages, particularly for early stages. For advanced stages, incorporation of tumor-related factors such as T stage might better elucidate survival differences and guide clinical treatment decisions.

Protocol registration

CRD 42023451793.