bluuebunny/crossref_metadata_2025 huggingface dataset:
How the use of an online healthcare community affects the doctor-patient relationship: An empirical study in China
Authors: Wang Zhanyou , Zhang Xin , Han Dongmei , Zhao Yaopei , Ma Liang , Hao Feifei
Published: April 2023

Possible improvements to the doctor-patient relationship are an important subject confronting national healthcare policy and health institutions. In recent years, online healthcare communities have changed the ways in which doctors and patients communicate, especially during the COVID-19 pandemic. However, previous research on how usage of online healthcare communities has affected the doctor-patient relationship is rather limited. This paper proposes a research model to investigate the relationship between online healthcare community usage and the doctor-patient relationship. An analysis of 313 patients’ data using structural equation modeling showed the following. First, the use of an online healthcare community has a positive impact on doctor-patient communication, helps improve the performance of healthcare procedures, and reduces healthcare costs. Second, doctor-patient communication and healthcare costs have a positive impact on patients’ emotional dependence and patients’ perception of healthcare quality, while healthcare procedures do not have this impact. Finally, patients’ emotional dependence and perception of healthcare quality have a positive effect on doctor-patient relationship through the mediator of patients’ satisfaction.
Association between adverse oral conditions and cognitive impairment: A literature review
Authors: Wei Tianhao , Du Yifeng , Hou Tingting , Zhai Chunjuan , Li Yuqi , Xiao Wei , Liu Keke
Published: April 2023

Oral environment deterioration results from a lack of self-cleaning ability in patients with cognitive dysfunction but is also a risk factor for cognitive dysfunction. Adverse oral conditions can be alleviated and improved through a self-management and medical examination. In this review, the epidemiological evidence of previous studies is integrated to highlight the relationship between periodontitis, tooth loss, oral flora, oral dysfunction and cognitive dysfunction, emphasizing the importance of oral health for cognition. The results show that poor oral condition is associated with cognitive impairment. Although many previous studies have been conducted, there is a lack of higher-level research evidence, different judgment criteria, and conflicting research results. There is a bidirectional relationship between oral health and cognitive dysfunction. A comprehensive analysis of the relationship between oral health and cognitive dysfunction that explores the relationship and takes measures to prevent cognitive dysfunction and control the progression of such diseases is warranted in the future.
Rural-to-urban migration, socio-economic status and cardiovascular diseases risk factors among Bangladeshi adults: A nationwide population based survey
Authors: Mumu Shirin Jahan , Stanaway Fiona F. , Merom Dafna
Published: April 2023

Background

Rural-to-urban migration is one of the key drivers of urbanization in Bangladesh and may impact on cardiovascular diseases (CVD) risk due to lifestyle changes. This study examined whether CVD risk factors were associated with migration to and duration of urban life, considering socio-economic indicators.

Methods

A total of 27,792 participants (18–59 years) from the 2006 Bangladesh cross-sectional Urban Health Survey were included in the analyses of whom 14,167 (M: 7,278; W: 6,889) were non-migrant urban residents and 13,625 (M: 6,413; W: 7,212) were rural-to-urban migrants. Gender-specific prevalence of CVD risk factors were estimated for urban and migrant groups. Multivariate logistic regression models were used to test the association between each CVD risk by education and wealth within each study group and their possible effect modification. An analysis on the rural-to-urban migrant subgroup only was conducted to examine the association between each CVD risk factor and length of urban stay adjusted for demographic and socio-economic indicators.

Results

Compared to urban residents, migrants had significantly lower prevalence of overweight/obesity for both genders. Hypertension was higher among urban women while alcohol/illicit drug use was higher among urban men. Mental health disorders were higher among migrants than urban residents for both genders and no difference were noted for diabetes or cigarette smoking prevalence. In both study groups and genders, the risk of overweight/obesity, hypertension and diabetes increased with increasing education and wealth whereas for mental health disorders, alcohol/illicit drug use, cigarette and bidi smoking the reverse was found. Differences in BMI between migrant and urban women were attenuated with increased education levels (p = 0.014 for interaction). Consistent increasing pattern of risk was observed with longer duration of urban stay; in migrant men for obesity (OR = 1.67), smoking (OR = 1.67) and alcohol/illicit drug use (OR = 2.86), and for obesity and mental health disorder among migrant women.

Conclusions

Migrants had high proportion of CVD risk factors which were influenced by education, wealth and duration of urban stay.
Qualitative analysis of front-of package labeling policy interactions between stakeholders and Health Canada
Authors: Jawad Aalaa , Mulligan Christine , Savona Natalie , L'Abbé Mary R.
Published: April 2023

Background

Front-of-package labelling regulations proposed by Health Canada in their Healthy Eating Strategy (2016) were finally passed in 2022, but remain unimplemented. This study analyzed interactions that occurred between stakeholders and government related to this policy proposal to identify key themes and policy implications.

Methods

A qualitative framework analysis was conducted on publicly available documents for stakeholder correspondences related to front-of-package that occurred between 2016 and 2019 in Health Canada’s Meetings and Correspondence on Healthy Eating database. Five sequential steps were applied: familiarization, identifying a thematic framework, indexing, charting, and mapping and interpretation. A complex systems (i.e., a dynamic system with multiple interconnecting components) lens was incorporated in the final step to deepen the analysis.

Results

Hundred and seventy-three documents were included, the majority from industry stakeholders (n = 108, 62.4%). Three overarching themes were identified: industry trying to control the agenda and resist regulation; questioning the evidence supporting the policy and its impact on the agri-food industry; and dismissing the need and effectiveness of the policy. Incorporating a complex system lens found industry and non-industry stakeholders held markedly different perspectives on how cohesive the system defined by the front-of-package labelling policy was, and the policy impact on its stability. Economic and opportunity costs were the main trade-offs, and symbol misinterpretation considered an unintended consequence by industry. Finally, some stakeholders argued for wider policy scope incorporating more products, while others requested a narrower approach through exemptions.

Conclusion

Interactions with industry stakeholders on health food policy proposals require careful consideration, given it may suit their interests to generate delays and policy discordance. Explicitly setting out the principles of engagement and actively encouraging non-industry stakeholder representation provides a more balanced approach to policy consultation and development.
Achieving descriptive accuracy in explanations via argumentation: The case of probabilistic classifiers
Authors: Albini Emanuele , Rago Antonio , Baroni Pietro , Toni Francesca
Published: April 2023

The pursuit of trust in and fairness of AI systems in order to enable human-centric goals has been gathering pace of late, often supported by the use of explanations for the outputs of these systems. Several properties of explanations have been highlighted as critical for achieving trustworthy and fair AI systems, but one that has thus far been overlooked is that of descriptive accuracy (DA), i.e., that the explanation contents are in correspondence with the internal working of the explained system. Indeed, the violation of this core property would lead to the paradoxical situation of systems producing explanations which are not suitably related to how the system actually works: clearly this may hinder user trust. Further, if explanations violate DA then they can be deceitful, resulting in an unfair behavior toward the users. Crucial as the DA property appears to be, it has been somehow overlooked in the XAI literature to date. To address this problem, we consider the questions of formalizing DA and of analyzing its satisfaction by explanation methods. We provide formal definitions of naive, structural and dialectical DA, using the family of probabilistic classifiers as the context for our analysis. We evaluate the satisfaction of our given notions of DA by several explanation methods, amounting to two popular feature-attribution methods from the literature, variants thereof and a novel form of explanation that we propose. We conduct experiments with a varied selection of concrete probabilistic classifiers and highlight the importance, with a user study, of our most demanding notion of dialectical DA, which our novel method satisfies by design and others may violate. We thus demonstrate how DA could be a critical component in achieving trustworthy and fair systems, in line with the principles of human-centric AI.
An online learning algorithm for adapting leg stiffness and stride angle for efficient quadruped robot trotting
Authors: Aboufazeli Mahtab , Samare Filsoofi Ali , Gurney Jason , Meek Sanford G. , Mathews V John
Published: April 2023

Animals adjust their leg stiffness and stride angle in response to changing ground conditions and gait parameters, resulting in improved stability and reduced energy consumption. This paper presents an online learning algorithm that attempts to mimic such animal behavior by maximizing energy efficiency on the fly or equivalently, minimizing the cost of transport of legged robots by adaptively changing the leg stiffness and stride angle while the robot is traversing on grounds with unknown characteristics. The algorithm employs an approximate stochastic gradient method to change the parameters in real-time, and has the following advantages: (1) the algorithm is computationally efficient and suitable for real-time operation; (2) it does not require training; (3) it is model-free, implying that precise modeling of the robot is not required for good performance; and (4) the algorithm is generally applicable and can be easily incorporated into a variety of legged robots with adaptable parameters and gaits beyond those implemented in this paper. Results of exhaustive performance assessment through numerical simulations and experiments on an under-actuated quadruped robot with compliant legs are included in the paper. The robot platform used a pneumatic piston in each leg as a variable, passive compliant element. Performance evaluation using simulations and experiments indicated that the algorithm was capable of converging to near-optimal values of the cost of transport for given operating conditions, terrain properties, and gait characteristics with no prior knowledge of the terrain and gait conditions. The simplicity of the algorithm and its demonstrably improved performance make the approach of this paper an excellent candidate for adaptively controlling tunable parameters of compliant, legged robots.
A robust method for approximate visual robot localization in feature-sparse sewer pipes
Authors: Edwards S. , Zhang R. , Worley R. , Mihaylova L. , Aitken J. , Anderson S. R.
Published: April 2023

Buried sewer pipe networks present many challenges for robot localization systems, which require non-standard solutions due to the unique nature of these environments: they cannot receive signals from global positioning systems (GPS) and can also lack visual features necessary for standard visual odometry algorithms. In this paper, we exploit the fact that pipe joints are equally spaced and develop a robot localization method based on pipe joint detection that operates in one degree-of-freedom along the pipe length. Pipe joints are detected in visual images from an on-board forward facing (electro-optical) camera using a bag-of-keypoints visual categorization algorithm, which is trained offline by unsupervised learning from images of sewer pipe joints. We augment the pipe joint detection algorithm with drift correction using vision-based manhole recognition. We evaluated the approach using real-world data recorded from three sewer pipes (of lengths 30, 50 and 90 m) and benchmarked against a standard method for visual odometry (ORB-SLAM3), which demonstrated that our proposed method operates more robustly and accurately in these feature-sparse pipes: ORB-SLAM3 completely failed on one tested pipe due to a lack of visual features and gave a mean absolute error in localization of approximately 12%–20% on the other pipes (and regularly lost track of features, having to re-initialize multiple times), whilst our method worked successfully on all tested pipes and gave a mean absolute error in localization of approximately 2%–4%. In summary, our results highlight an important trade-off between modern visual odometry algorithms that have potentially high precision and estimate full six degree-of-freedom pose but are potentially fragile in feature sparse pipes, versus simpler, approximate localization methods that operate in one degree-of-freedom along the pipe length that are more robust and can lead to substantial improvements in accuracy.
Limitations of the circular economy concept in law and policy
Authors: Steenmans Katrien , Lesniewska Feja
Published: April 2023

The circular economy (CE) concept has gained significant attention globally and nationally largely due to anticipated economic, environmental, and social benefits for sustainable development. As a result, laws and policies to advance CEs are increasingly being adopted nationally and regionally. In this perspective article we argue that a systematic approach to designing and implementing CE laws is necessary to cover the multi-level, multi-actor, and multi-sector dimensions of the CE concept. Such an approach should be built on a CE concept with clarified boundaries and scale to ensure it remains a meaningful concept and avoids perpetuating an unsustainable status quo. Moreover, a systematic approach should incorporate justice dimensions to deliver CEs that are fair, just, and inclusive. In the article we first identify five fundamental limitations to existing CE laws: (1) unclear boundaries and scale, (2) oversimplification of goals, (3) side-lining of justice dimensions, (4) reinforcement of the status quo, and (5) unintended consequences. Secondly, based on these limitations, we identify four directions for further research that can contribute to developing more effective CE laws. As there is no one-size-fits-all CE approach, any changes to existing laws and policies, or development of new ones, will require a wider evidence-base, from both the Global South and marginalized communities in the Global North, to reflect, inter alia, different practices, cultural relationships with material flows, and epistemological diversity.
What are the Benefits of Learning a Second Language?
Authors: Xie Xiaoyi , Zhou Yu , Lu Kehan , Privitera Adam John
Published: April 2023

Bilingualism, which means speaking more than one language, allows a person to communicate with a larger number of people. Some research suggests that speaking more than one language may also improve brain function. We wanted to see whether the relationship between improved brain function and being bilingual differed based on how well or how much a person uses their second language. To ask our question, we recruited a group of high school students who spoke both Mandarin and English. Because speaking two languages is thought to improve how well a person performs on certain tasks, we thought that the students who were better at their second language would have better performance on those tasks. Our findings support the idea that developing your second language, especially if you speak it well, can improve the way your brain functions. Keep reading to learn about our research!
MAVEN mission perspectives and approaches to inclusion
Authors: Curry S. M.
Published: April 2023

The Mars Atmosphere and Volatile Evolution mission (MAVEN) is a NASA spacecraft that has been orbiting Mars since 2014. The Mars Atmosphere and Volatile Evolution mission team has established a current set of best practices to strengthen Diversity, Equity, Inclusion, and Accessibility (DEIA) initiatives; there are numerous axes of diversity, and this paper does not focus on one specific aspect of diversity but rather focuses on mission-specific approaches to inclusion. We present the past and present approaches as well as future initiatives and areas of improvement to continuing our efforts towards maximizing inclusion and engagement on the Mars Atmosphere and Volatile Evolution mission team and its working environment. The approaches presented in this paper are applicable to the space physics and planetary science communities, as well as any large-scale science or mission teams.
A figurational approach to soft power and sport events. The case of the FIFA World Cup Qatar 2022™
Authors: Næss Hans Erik
Published: April 2023

Soft power' is a term often used to explain how states seek to be appealing and attractive to others to increase their geopolitical influence instead of using military force or economic threats. As part of a soft power agenda, sport events showcase the values, culture and imagery of a host nation, and through that, ideally, attract investors, tourists and attention. But there are problems with the concept, especially when it is adopted by sport events where it is used as a metaphor, as a heuristic device, and a descriptor of current affairs, to name a few. In particular, the concept does not necessarily capture the accumulation of tit-for-tat strategies and bargaining in geopolitics where sport events are involved. In contrast, by using the FIFA World Cup Qatar 2022™ as key example and drawing upon Norbert Elias' figurational sociology as source of theoretical refinement, this article discusses how soft power can become more precise as an analytical category in a context of sport and geopolitics.
Mechanical ventilation in dogs and cats with tick paralysis
Authors: O'Keeffe Tess , Donaldson Rebekah E.
Published: April 2023

Respiratory failure from tick paralysis (TP) is an important cause of mortality in cats and dogs in Australia, occurring from a combination of respiratory muscle paralysis, upper respiratory tract obstruction and pulmonary disease. Patients may require positive-pressure ventilation in management of any combination of hypoxemia, hypoventilation or respiratory fatigue, but may also require airway management due to laryngeal paralysis. No single ventilation strategy is recommended due to the heterogenous disease presentations. Lung protective ventilation should be used in patients with pulmonary disease. Due to local and systemic effects of TP, patients are at higher risk of complications such as aspiration pneumonia and corneal ulceration and may have additional intravenous fluid and nutritional considerations. Treatment with hyperimmune serum is associated with improved outcomes. Prognosis is considered good with documented survival to discharge (STD) of 52.6–77% for animals with TP ventilated with lung disease and 90.5% for animals without lung disease. Median reported duration of ventilation for TP ranges from 23 to 48 h (range 3 h−10 days). The severity of individual neuromuscular signs and the presence of associated conditions such as aspiration pneumonia and laryngeal paralysis may necessitate longer periods of mechanical ventilation. This review aims to summarize the current recommendations regarding indications, management and prognosis of cats and dogs undergoing MV for TP and to identify areas for future research.
Comparison of radiographic scoring systems for assessment of bone healing after tibial plateau leveling osteotomy in dogs
Authors: Leal R. A. , Lambrechts N. E. , Crowley J. D. , Griffin J. F. , Karnia J. J. , Torres B. T. , Maritato K. C. , Kieves N. R. , Duerr F. M.
Published: April 2023

Introduction

Accurate radiographic assessment of bone healing is vital in determining both clinical treatment and for assessing interventions aimed at the promotion of bone healing. Several scoring systems have been used to evaluate osteotomy changes following tibial plateau leveling osteotomy (TPLO). The goal of this study was to compare the ability of five radiographic scoring systems to identify changes in bone healing following TPLO over time (Aim I), and to evaluate the influence of limb positioning on TPLO osteotomy scoring (Aim II).

Materials and methods

Phase I-A randomized, blinded, prospective study was conducted using similarly positioned postoperative TPLO radiographs from seven dogs taken immediately postoperatively, 6-weeks, and 8-weeks postoperatively. Ten reviewers assessed the radiographs, and five different scoring systems were tested for each set including three previously published ones, a Visual Analog Score (VAS), and a subjective 11-point scale. For each system, responses for 6-week postoperative were compared to 8-week postoperative. Scores were judged as correct (=showing an increase in score), incorrect (=decrease in score), or unchanged (=same score). Phase II-An international group of 39 reviewers was asked to score radiographs from three dogs, taken in different positions, using the VAS grading system. Scores were averaged and comparisons were made for each set.

Results

Phase I-The VAS system identified the greatest number of sets correctly (76%), with the least unchanged scores (15%), and 9% incorrect scores. Phase II-All three patients had an increase in the average difference between VAS-scores for differently positioned radiographs compared to similarly positioned radiographs. The magnitude of change between different positions far exceeded the magnitude of comparison of the similarly positioned radiographs from the 6- and 8-week time point.

Discussion/Conclusion

The VAS system appears to be the most appropriate of the tested systems to identify small changes in bone healing. In addition, the positioning of postoperative TPLO radiographs makes a substantial difference in the healing score that is assigned. Care must be undertaken when performing postoperative radiographs in both the clinical and research setting to ensure accurate assessment of bone healing.
Dynamic Modeling and Attitude–Vibration Cooperative Control for a Large-Scale Flexible Spacecraft
Authors: He Guiqin , Cao Dengqing
Published: April 2023

Modern spacecraft usually have larger and more flexible appendages whose vibration becomes more and more prominent, and it has a great influence on the precision of spacecraft attitude. Therefore, the cooperative control of attitude maneuvering and structural vibration of the system has become a significant issue in the spacecraft design process. We developed a low-dimensional and high-precision mathematical model for a large-scale flexible spacecraft (LSFS) equipped with a pair of hinged solar arrays in this paper. The analytic global modes are used to obtain the rigid–flexible coupling discrete dynamic model, and the governing equations with multiple DOFs for the system are derived by using the Hamiltonian principle. The rigid–flexible coupled oscillating responses of LSFS under the three-axis attitude-driving torque pulse during the in-orbit attitude maneuvering process are investigated. A study on the flexibility of the hinge was also conducted. Based on the simplified and accurate dynamic model of the system, we can obtain a state-space model for LSFS conveniently, and the cooperative control schemes for rigid motion and flexible oscillation control are designed by using the LQR, PD, and PD + IS algorithms. The simulation results show that three cooperative controllers can realize spacecraft attitude adjustment and synchronously eliminate flexible oscillation successfully. By comparison, the PD + IS controller is simpler so that it is suitable for the real-time attitude–vibration cooperative control of spacecraft.
Effect of Cropping Systems and Environment on Phenolic Acid Profiles and Yielding of Hybrid Winter Wheat Genotypes
Authors: Buczek Jan , Jańczak-Pieniążek Marta , Harasim Elżbieta , Kwiatkowski Cezary , Kapusta Ireneusz
Published: April 2023

Wheat is of significant economic importance due to its high yield potential and high nutritional value as well as the technological usefulness of the grain. Field experiments were carried out in the years 2015–2018 in southeastern Poland. A three-factor experiment was used to study the influence of wheat cultivars (hybrid—cvs. Hybred and Hymack; common—cv. Batuta), cultivation systems (organic—ORG, integrated—INT, conventional—CON) and of environmental conditions (using two different locations: Dukla and Nowy Lubliniec) on wheat grains’ phenolic acid (PA) content and grain yield. The research confirms the genetic determinants of grain yield and PA composition in wheat grains, and their different accumulation levels of ferulic acid and other PAs—with the exception of sinapic, p-coumaric, and salicilic—with hybrid cultivars performing better than common cultivars. The ORG system, compared to the INT and CON systems, caused a larger increase in total acids (TPAs)—especially of ferulic, vanillic, and syringic acids—in grains of cv. Hybred, and of caffeic acid in cv. Hymack, compared to cv. Batuta. The lack of interaction between the cultivation systems and the cultivars indicates that similar reactions to increases in grain yield due to increases in the intensity of cultivation take place in cultivars. The more favourable environmental conditions in Dukla favoured the accumulation of ferulic, p-coumaric, vanillic, syringic, p-hydroxybenzoic, and protocatechuic acids in the grains. TPAs were higher by 4.3% and the grain yield by 4.0% on average. Variable conditions in the season 2015/2016 resulted in higher yields of hybrid cvs. grains than of common cv., which proves the greater yielding stability of these cultivars in years with adverse weather conditions. The season 2016/2017 had less rainfall and faced high temperatures during grain ripening, favouring a higher PA content and TPAs, especially in the grains of cv. Hybred. This suggests a need to further assess the genetic progress of hybrid wheat cultivars cultivated under different environmental conditions in terms of their PA composition and content.
Remote Monitoring of Crop Nitrogen Nutrition to Adjust Crop Models: A Review
Authors: Silva Luís , Conceição Luís Alcino , Lidon Fernando Cebola , Maçãs Benvindo
Published: April 2023

Nitrogen use efficiency (NUE) is a central issue to address regarding the nitrogen (N) uptake by crops, and can be improved by applying the correct dose of fertilizers at specific points in the fields according to the plants status. The N nutrition index (NNI) was developed to diagnose plant N status. However, its determination requires destructive, time-consuming measurements of plant N content (PNC) and plant dry matter (PDM). To overcome logistical and economic problems, it is necessary to assesses crop NNI rapidly and non-destructively. According to the literature which we reviewed, it, as well as PNC and PDM, can be estimated using vegetation indices obtained from remote sensing. While sensory techniques are useful for measuring PNC, crop growth models estimate crop N requirements. Research has indicated that the accuracy of the estimate is increased through the integration of remote sensing data to periodically update the model, considering the spatial variability in the plot. However, this combination of data presents some difficulties. On one hand, at the level of remote sensing is the identification of the most appropriate sensor for each situation, and on the other hand, at the level of crop growth models is the estimation of the needs of crops in the interest stages of growth. The methods used to couple remote sensing data with the needs of crops estimated by crop growth models must be very well calibrated, especially for the crop parameters and for the environment around this crop. Therefore, this paper reviews currently available information from Google Scholar and ScienceDirect to identify studies relevant to crops N nutrition status, to assess crop NNI through non-destructive methods, and to integrate the remote sensing data on crop models from which the cited articles were selected. Finally, we discuss further research on PNC determination via remote sensing and algorithms to help farmers with field application. Although some knowledge about this determination is still necessary, we can define three guidelines to aid in choosing a correct platform.
The γ-Aminobutyric Acid (GABA) Synthesis Gene Regulates the Resistance to Water Core-Induced Hypoxia Stress for Pear Fruits
Authors: Liu Xiao , Ma Hao , Liu Jing , Liu Donghe , Wang Chunlei
Published: April 2023

Watercore is a physiological disorder which often occurs in Rosaceae fruits, and it causes hypoxia stress, promoting fruit decay. γ-aminobutyric acid (GABA) was reported as being involved in different abiotic stresses, and glutamate decarboxylase (GAD) is the key enzyme of GABA synthesis in plants. Our previous transcriptome analysis found that PpGAD2 was significantly induced in watercore fruit; however, the mechanism through which PpGAD2 regulates watercore-induced hypoxia stress resistance in pears is unclear. The present study found that the fruit pulp ethanol, malondialdehyde (MDA) and H2O2 content was significantly inhibited by exogenous GABA. The transcript abundance of PpGAD2 was significantly higher than that of other PpGADs in watercore fruit or healthy fruit. Tissue expression showed that the content of PpGAD2 in mature fruit was higher than in young fruit. Moreover, subcellular localization showed that PpGAD2 was located in the cytoplasm. Transient overexpression assays suggested that PpGAD2 had a role in GABA synthesis. Several CML (calmodulin–like) genes were also significantly increased in watercore fruit. Moreover, PpWRKY53 was significantly induced in watercore fruit, and the GUS activity assay showed that PpWRKY53 can significantly increase the activity of the PpGAD2 promoter. Taken together, these results demonstrate that PpGAD2 played an important role in GABA synthesis to increase plants’ resistance to hypoxia stress, and its activity may be affected by PpWRKY53 and several watercore-induced CML genes.
Impacts of Partial Substitution of Chemical Fertilizer with Organic Manure on the Kinetic and Thermodynamic Characteristics of Soil β–Glucosidase
Authors: Dong Ruixiao , Abdelkerim-Ouba Djido , Liu Danyang , Ma Xianfa , Wang Shuang
Published: April 2023

To study the characteristics of the β–glucosidase enzymatic reaction in wheat field soil under the condition of reducing the application of chemical fertilizer, five fertilization treatments were established, including no fertilizer (CK), chemical fertilizer (F), organic fertilizer (OF), 25% organic fertilizer plus 75% chemical fertilizer (25% OF), and 50% organic fertilizer plus 50% chemical fertilizer (50% OF). The activity of β–glucosidase and its kinetic and thermodynamic characteristics were analyzed by using microplate p–nitrophenol colorimetry. The results showed that the Vmax values of soil β–glucosidase in the organic substitution of chemical fertilizer treatment were higher than those in the chemical fertilizer and no fertilizer treatments, and the Km values were lower than those in the chemical fertilizer and no fertilizer treatments at the different growth stages. The Vmax value in the 25% OF treatment was the highest at the jointing stage and that of the OF treatment was the highest at the booting stage; the Km value in the 50% OF treatment was the lowest at the different growth stages. Compared with the chemical fertilizer and no fertilizer treatments, the application of organic fertilizer effectively reduced thermodynamic parameters such as Ea, Q10, ∆H, ∆G, and ∆S at the jointing and booting stages of wheat. The thermodynamic parameters in the 25% OF treatment were the lowest at the jointing stage and those in the OF treatment were the lowest at the booting stage. A reasonable amount of organic fertilizer is more beneficial to enzymatic reactions and improves the soil quality and the ability to supply nutrients to wheat cultivation.
Sustainable Management of Sewage Sludge Using Dhaincha (Sesbania bispinosa (Jacq.) W.Wight) Cultivation: Studies on Heavy Metal Uptake and Characterization of Fibers
Authors: Širić Ivan , AL-Huqail Arwa A. , Kumar Pankaj , Goala Madhumita , Abou Fayssal Sami , Adelodun Bashir , Ajibade Fidelis O. , Alrumman Sulaiman A. , Alamri Saad A. M. , Taher Mostafa A. , Singh Jogendra , Kumar Vinod , Eid Ebrahem M.
Published: April 2023

In this study, the potential use of sewage sludge (SS) as a fertilizer source for cultivated Dhaincha (Sesbania bispinosa (Jacq.) W.Wight) crops was investigated. Field experiments were conducted using different doses of SS such as 0% (T0), 5% (T1), 10% (T2), and 15% (T3) to amend the soil (w/w). The findings showed that soil amended with SS significantly (p < 0.05) improved the response parameters of S. bispinosa with an increase in the dose of SS from 0% to 15%. In particular, the T3 treatment gave the best results (mean values) for plant growth and yield parameters such as plant height (380.59 cm), fresh weight (1.47 kg/plant), dry weight (0.27 kg/plant), base diameter (3.19 cm), seed yield (44.71 g/plant), total chlorophyll (3.15 mg/g fwt), carotenoids (0.88 mg/g fwt), carbohydrates (9.40 mg/g), and phenol (0.13 mg/g) contents. Moreover, the selected proximate and fiber characteristics of the S. bispinosa crop were significantly (p < 0.05) improved by the same treatment (T3); such as ash (7.25%), crude fiber (32.70%), crude protein (15.94%), lignin (24.60%), cellulose (37.25%), fiber weight (3.06 g/plant), stick weight (32.08 g/plant), fiber: stick ratio (0.10), fiber diameter (26.97 µm), ultimate tensile strength (855.98 MPa), strength (58.92 g/tex), density (1.54 g/cm3), and luster (45.65%) compared with the control treatment (T0). The bioaccumulation factor (BAF) studies showed that the S. bispinosa plant was capable of accumulating selected heavy metals from the soil following the order: Fe > Mn > Zn > Cu > Cd > Cr. The study suggested a sustainable approach for efficient soil fertilization and high-quality S. bispinosa fiber production that could minimize the environmental pollution caused by the unsafe disposal of SS.
Geochemical Characteristics of Typical Karst Soil Profiles in Anhui Province, Southeastern China
Authors: Ji Wenbing , Lu Yuanyuan , Yang Min , Wang Jian , Zhang Xiaoyu , Zhao Caiyi , Xia Bing , Wu Yunjin , Ying Rongrong
Published: April 2023

The geographical distributions of Cd and several other heavy metals (HMs) (Hg, Cu, Ni, Pb, Zn, Cr, As, Co, and V) were characterized in 90 (p > 0.05) terra rossa samples across the Anhui karst area. Significant enrichment of HM was observed in this soil, mainly associated with the weathering of Cd-enriched carbonate rocks. Then, this enrichment was developed in 31 profiles. Our investigations revealed pedogenic processes as the dominant factors accounting for the enrichment of Hg, Cu, Ni, As, Co, and V. We also observed that all soil samples had a silty clay texture, with a pH scope of 4.08–8.04 and a median value of 6.50. In addition, the soil samples had relatively high saturation, with basic cations over 6.68%. The enrichment of the HMs based on their distinct factors were as follows: Cd (3.92) > As (2.55) > Zn (1.62) > Ni (1.50) > Cu (1.47) > Pb (1.47) > V (1.43) > Cr (1.23) > Co (1.19) > Hg (1.12). Finally, terra rossa samples derived from carbonate rocks were categorized as Cambisols, Luvisols, and Regosols. The soil profiles of Cambisols and Luvisolsis were less developed, so the HM concentrations were relatively low. The Regosols profile contained the highest total Cd concentration and exhibited a higher capacity to immobilize Cd compared with other soil profiles. Regosols are also characterized by high pH values (scope of 7.05 to 8.22, with an average value of 7.56). The contents of HM also exhibited minor changes across the Regosol, Cambisol, and Luvisol profiles, implying that the karst development degrees of weathering in Anhui were relatively low.
Starch and Dough-Related Properties of Wheat (Triticum aestivum L.) Exposed to Varying Temperatures and Radiances after Anthesis
Authors: Zhang Zhenzhen , Jia Dinghan , Wang Dexin , Zhou Nianbing , Xing Zhipeng , Xu Ke , Wei Haiyan , Guo Baowei , Zhang Hongcheng
Published: April 2023

With the increase in global warming and solar radiation, the starch quality and dough properties of wheat have also been affected to some negative extent. To prepare for the effects of future climate change, we explored the effects of different temperatures and radiation on the properties of wheat starch and dough. Six sowing dates for four wheat varieties were selected for this study. The seedling population on the first sowing date (S1) was 300 × 104 plants ha−1, which increased by approximately 10% after the subsequent sowing date. The results showed that a delay in sowing time increased the daily mean temperature after anthesis and decreased the effective accumulated temperature and cumulative solar radiation. When the daily mean temperature increased 0.32 °C after anthesis, the total starch content decreased 1.2%, the sedimentation value increased by 14.19 s, the water absorption increased 0.62%, the dough development time increased 0.4 min, and the dough stability time increased 0.28 min. The gelatinization and dough properties of wheat starch were not only related to the daily mean temperature, but were also significantly related to the effective accumulated temperature and cumulative solar radiation. The results showed that the starch and dough properties of wheat are also related to the cumulative radiation temperature after anthesis. Compared with radiation, temperature is the main climatic factor affecting the formation of wheat starch and dough.
Net Portal Appearance of Amino Acids in Iberian and Landrace Pigs Fed Different Protein Content in the Diet
Authors: Lachica Manuel , Rodríguez-López José Miguel , González-Valero Lucrecia , Fernández-Fígares Ignacio
Published: April 2023

Iberian pigs have low rates of muscle protein deposition compared with modern breeds. Differences in net portal appearance (NPA) of amino acids (AA) might partially explain that. NPA of AA was measured in six Iberian and six Landrace gilts (28 kg) fitted with catheters in portal and mesenteric (para-aminohippuric acid infusion) veins, and carotid artery. Blood samples from porta and artery were simultaneously taken at 0, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 5, and 6-h after feeding two isoenergetic diets (14–14.5 MJ metabolizable energy/kg dry matter) with different crude protein (145 (LCP) and 187 (HCP) g/kg dry matter) content. NPA of essential AA (EAA) and non-essential AA (NEAA) was lower (p < 0.05) in Iberian than Landrace pigs, and in LCP than HCP diet. Fractional absorption (NPA/AA intake) of EAA, NEAA, and total AA was, respectively, 36, 49, and 44% lower in LCP than HCP diet in Iberian pigs; and 8, 2, and 4% greater in Landrace pigs. Fractional absorption of EAA, NEAA, and total AA was 42, 68, and 60% lower in Iberian than Landrace pigs fed LPC diet; and 1, 36, and 26% when fed the HCP diet. NPA of AA may partially explain the low growth rate of Iberian pigs.
Euthanasia and Pain in Canine Patients with Terminal and Chronic-Degenerative Diseases: Ethical and Legal Aspects
Authors: Mota-Rojas Daniel , Domínguez-Oliva Adriana , Martínez-Burnes Julio , Casas-Alvarado Alejandro , Hernández-Ávalos Ismael
Published: April 2023

Euthanasia is commonly performed in veterinary medicine to humanely induce the death of an animal when its quality of life is affected by pain or chronic degenerative diseases. The choice of euthanasia is a bilateral decision that represents a challenge for both the veterinarian and the owner of the animal due to the close emotional human–animal bond. Currently, there is legislation that can orient veterinarians concerning euthanasia and the causes that would justify this resolution. However, it is still controversial, and deciding it as the last available resort requires considering it from a medical, legal, and moral perspective. Therefore, this review aims to explore the ethical and legal implications of euthanasia in canine patients. It will analyze the reason that can justify euthanasia in animals with pain or terminal and chronic degenerative diseases, highlighting the importance of effective communication, ethical knowledge, and consideration of euthanasia as a multimodal resolution.
Nutraceutical Effect of Resveratrol on the Mammary Gland: Focusing on the NF-κb /Nrf2 Signaling Pathways
Authors: Malik Muhammad Umair Ul Hassan , Hashmi Nighat , Khan Marium , Aabdin Zain ul , Sami Rokayya , Aljahani Amani H. , Al-Eisa Rasha A. , Moawadh Mamdoh S. , Algehainy Naseh A.
Published: April 2023

The aim of this study is to evaluate the defensive role of resveratrol, which is antagonistic to the oxidative stress and inflammation that is prompted by LPS in mammary tissue of female mice. Thirty adult mice were distributed into three groups (n = 10) control (CON), lipopolysaccharides at 2.5 mg/kg (LPS), and lipopolysaccharides at 2.5 mg/kg with 2 mg/kg of resveratrol (RES + LPS). The treatments were applied for 15 consecutive days. Spectrophotometry was used to quantify ROS in the blood, and proinflammatory cytokines concentrations were determined through radioimmunoassay. NF-κB, Jnk, IL-1β, Erk, IL-6, Nrf2 and TNF-α were quantified by RT-qPCR, and Western blots were used to quantifyP65 and pP65 protein intensities. MDA production was considerably increased, and the activity of T-AOC declined in the LPS treatment in comparison with the CON group but was significantly reversed in the RES + LPS group. Proinflammatory cytokines production and the genes responsible for inflammation and oxidative stress also showed higher mRNA and pP65 protein intensity in the LPS group, while Nrf2 showed a remarkable decline in mRNA expression in the LPS versus the CON group. All these mRNA intensities were reversed in the RES + LPS group. There were no remarkable changes in P65 protein intensity observed between the CON, LPS, and RES + LPS groups. In conclusion, resveratrol acts as a protective agent to modulate cellular inflammation and oxidative stress caused by LPS in mammary tissue of female mice.
Nutrient Intake, Performance, Carcass Characteristics, Meat Quality, and Cost Analysis of Sheep Submitted to Intermittent Supplementation on Masai Grass Pastures
Authors: Urbano Stela Antas , Rodrigues Jessica Caroline Nascimento , Ribeiro Pedro Henrique Cavalcante , Silva Yasmin dos Santos , da Silva Rodrigo Ferreira , Neto João Virgínio Emerenciano , Rangel Adriano Henrique do Nascimento , de Oliveira Juliana Paula Felipe , de Medeiros Henrique Rocha
Published: April 2023

This study aimed to assess nutrient intake, performance, intake behavior, carcass characteristics, and meat quality of sheep managed on Masai grass pastures along with intermittent concentrate supplementation, in addition to the economic impacts of applying that strategy. A sample of 24 Santa Inês sheep (12 males and 12 females) was kept for 80 days on Panicum maximum cv. Massai pastures and was supplemented daily or every other day. The voluntary intake of dry matter and nutrients was estimated. Mean daily weight gain and total weight gain were calculated and intake behavior parameters were assessed. The males were slaughtered and the weight and yield of the carcasses and meat cuts were assessed. The meat was analyzed for cooking losses, shear force, and color. The gross margin of the system was estimated from the difference between total income and operational cost. No effect was seen of the interaction between supplementation frequency and sex for any parameter assessed (p > 0.05). Intermittent supplementation of Santa Inês sheep managed with Masai grass is recommended since the strategy does not impact nutrient intake, performance, intake behavior, carcass characteristics, or meat quality, but lowers the costs of producing 1 kg of meat by 15%.
The Spread of Peste Des Petits Ruminants Virus Lineage IV in West Africa
Authors: Couacy-Hymann Emmanuel , Berete Kouramoudou , Odoom Theophilus , Zerbo Lamouni Habibata , Mathurin Koffi Yao , Kouakou Valère Kouame , Doumbouya Mohamed Idriss , Balde Aminata , Ababio Patrick Tetteh , Ouoba Lalidia Bruno , Guigma Dominique , Drobo Adama Dji-tombo , Guitti Mariétou , Johnson Sherry Ama Mawuko , Blavo David Livingstone Mawuko , Cattoli Giovanni , Lamien Charles E. , Dundon William G.
Published: April 2023

Monitoring the transboundary spread of peste des petits ruminants (PPR) virus is an essential part of the global efforts towards the eradication of PPR by 2030. There is growing evidence that Lineage IV is becoming the predominant viral lineage, replacing Lineage I and II in West Africa. As part of a regional investigation, samples collected in Burkina Faso, Côte d’Ivoire, Guinea and Ghana were screened for the presence of PPRV. A segment of the nucleoprotein gene from positive samples was sequenced, and phylogenetic analysis revealed the co-circulation of Lineage II and IV in Burkina Faso, Côte d’Ivoire and Guinea, and the identification of Lineage IV in Ghana. These data will be of importance to local and regional authorities involved in the management of PPRV spread.
Exploring Efflux as a Mechanism of Reduced Susceptibility towards Biocides and Fluoroquinolones in Staphylococcus pseudintermedius
Authors: Leal Marta , Morais Catarina , Ramos Bárbara , Pomba Constança , Abrantes Patrícia , Costa Sofia Santos , Couto Isabel
Published: April 2023

Staphylococcus pseudintermedius is the main bacterial cause of skin and soft tissue infections (SSTIs) in companion animals, particularly dogs. The emergence of methicillin-resistant S. pseudintermedius (MRSP) strains, frequently with multidrug resistance phenotypes is a public health concern. This study aimed to evaluate efflux, a resistance mechanism still poorly characterized in S. pseudintermedius, as a contributor to biocide and fluoroquinolone resistance. Susceptibility to the efflux pump substrates ethidium bromide (EtBr), tetraphenylphosphonium bromide (TPP) and ciprofloxacin (CIP) was evaluated by minimum inhibitory concentration (MIC) determination for 155 SSTIs-related S. pseudintermedius in companion animals. EtBr and TPP MIC distributions were analyzed to estimate cut-off (COWT) values. The effect of the efflux inhibitors (EIs) thioridazine and verapamil was assessed upon MICs and fluorometric EtBr accumulation assays, performed with/without glucose and/or EIs. This approach detected a non-wild type population towards TPP with increased efflux, showed to be strain-specific and glucose-dependent. Resistance to fluoroquinolones was mainly linked to target gene mutations, yet a contribution of efflux on CIP resistance levels could not be ruled out. In sum, this study highlights the relevance of efflux-mediated resistance in clinical S. pseudintermedius, particularly to biocides, and provides a methodological basis for further studies on the efflux activity on this important pathogen of companion animals.