bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Marinades Prepared from Food Industry By-Products on Quality and Biosafety Parameters of Lamb Meat
Authors: Zavistanaviciute Paulina , Klementaviciute Jolita , Klupsaite Dovile , Zokaityte Egle , Ruzauskas Modestas , Buckiuniene Vilija , Viskelis Pranas , Bartkiene Elena
Published: March 2023

This study aimed to develop marinade formulas based on by-products from the dairy, berry, and fruit industries and apply them to lamb meat (LM) treatments to improve the safety and quality characteristics of the meat. To fulfil this aim, six marinade (M) formulations were created based on acid whey (AW) fermented with Lacticaseibacillus casei (Lc) and Liquorilactobacillus uvarum (Lu), either alone or combined with freeze-dried apple (AP) or blackcurrant (BC) pomace. The most appropriate fermentation times for the marinades were selected according to the lower pH values and higher viable LAB counts in the samples. Additionally, the antimicrobial activity of the selected marinades against pathogenic and opportunistic bacterial strains was tested. The characteristics of the LM were analysed after 24 and 48 h of treatment, including physicochemical, technological, and microbiological parameters, as well as overall acceptability. It was established that, after 48 h of fermentation, all of the tested marinades, except M-AWLuBC, had lactic acid bacterial counts > 8.0 log10 CFU·mL−1 and pH values < 3.74. The broadest spectra of pathogen inhibition were observed in the M-AWLuAP and M-AWLuBC marinades. The latter formulations improved the water holding capacity (WHC) and overall acceptability of the LM, while, in the LM-AWLcAP samples, histamine, cadaverine, putrescine, tryptamine, and phenylethylamine were not formed. Lastly, LM treatment with the M-AWLcAP and M-AWLuAP formulas for 48 h achieved the highest overall acceptability (9.04 and 9.43), tenderness (1.53 and 1.47 kg·cm−2) and WHC (2.95% and 3.5%) compared to the control samples.
iTRAQ-Based Proteomic Analyses of Regulation of Isothiocyanate and Endogenous Selenium Metabolism in Broccoli Sprouts by Exogenous Sodium Selenite
Authors: Quan Xiaolan , Cheng Yuwei , Yang Zhengfei , Yang Jia , Fang Weiming , Yin Yongqi
Published: March 2023

Broccoli sprouts have high isothiocyanate and selenium accumulation capacity. This study used a combination of methods, including physiological and biochemical, gene transcription and proteomic, to investigate the isothiocyanate and endogenous selenium accumulation mechanisms in broccoli sprouts under exogenous sodium selenite treatment during germination. Compared with the control, the sprouts length of broccoli sprouts under exogenous selenium treatment was significantly lower, and the contents of total phenol and malondialdehyde in 6-day-old broccoli sprouts were substantially higher. The contents of isothiocyanate and sulforaphane in 4-day-old were increased by up-regulating the relative expression of genes of UGT74B1, OX-1, and ST5b. The relative expression of BoSultr1;1, BoSMT, BoHMT1, and BoCOQ5-2 genes regulating selenium metabolism was significantly up-regulated. In addition, 354 proteins in 4-day-old broccoli sprouts showed different relative abundance compared to the control under selenium treatment. These proteins were classified into 14 functional categories. It was discovered that metabolic pathways and biosynthetic pathways of secondary metabolites were significantly enriched. The above results showed that exogenous selenium was beneficial in inducing the accumulation of isothiocyanate and selenium during the growth of broccoli sprouts.
Nutritional Genomic Approach for Improving Grain Protein Content in Wheat
Authors: Kartseva Tania , Alqudah Ahmad M. , Aleksandrov Vladimir , Alomari Dalia Z. , Doneva Dilyana , Arif Mian Abdur Rehman , Börner Andreas , Misheva Svetlana
Published: March 2023

Grain protein content (GPC) is a key aspect of grain quality, a major determinant of the flour functional properties and grain nutritional value of bread wheat. Exploiting diverse germplasms to identify genes for improving crop performance and grain nutritional quality is needed to enhance food security. Here, we evaluated GPC in a panel of 255 Triticum aestivum L. accessions from 27 countries. GPC determined in seeds from three consecutive crop seasons varied from 8.6 to 16.4% (11.3% on average). Significant natural phenotypic variation in GPC among genotypes and seasons was detected. The population was evaluated for the presence of the trait-linked single nucleotide polymorphism (SNP) markers via a genome-wide association study (GWAS). GWAS analysis conducted with calculated best linear unbiased estimates (BLUEs) of phenotypic data and 90 K SNP array using the fixed and random model circulating probability unification (FarmCPU) model identified seven significant genomic regions harboring GPC-associated markers on chromosomes 1D, 3A, 3B, 3D, 4B and 5A, of which those on 3A and 3B shared associated SNPs with at least one crop season. The verified SNP–GPC associations provide new promising genomic signals on 3A (SNPs: Excalibur_c13709_2568 and wsnp_Ku_c7811_13387117) and 3B (SNP: BS00062734_51) underlying protein improvement in wheat. Based on the linkage disequilibrium for significant SNPs, the most relevant candidate genes within a 4 Mbp-window included genes encoding a subtilisin-like serine protease; amino acid transporters; transcription factors; proteins with post-translational regulatory functions; metabolic proteins involved in the starch, cellulose and fatty acid biosynthesis; protective and structural proteins, and proteins associated with metal ions transport or homeostasis. The availability of molecular markers within or adjacent to the sequences of the detected candidate genes might assist a breeding strategy based on functional markers to improve genetic gains for GPC and nutritional quality in wheat.
Distribution of Calcium, Phosphorus and Magnesium in Yak (Bos grunniens) Milk from the Qinghai Plateau in China
Authors: Franceschi Piero , Sun Wancheng , Malacarne Massimo , Luo Yihao , Formaggioni Paolo , Martuzzi Francesca , Summer Andrea
Published: March 2023

This research was aimed to assess the distribution of calcium, phosphorus and magnesium within the casein micelles of yak milk. To this aim, nine bulk yak milk samples (Y-milk), collected in three yak farms located in the Chinese province of Qinghai, were compared to nine bulk cow milk samples used as a reference. A quite similar content of colloidal calcium (0.80 vs. 0.77 mmol/g of casein; p > 0.05), a higher content of magnesium (0.05 vs. 0.04 mmol/g of casein; p ≤ 0.01) and a lower content of colloidal phosphorus (0.48 vs. 0.56 mmol/g of casein; p ≤ 0.01) between yak and cow casein micelles were found. Moreover, the yak casein micelles showed a lower value of prosthetic phosphorus (0.20 vs. 0.26 mmol/g of casein; p ≤ 0.05) compared to the cow micelles. The lower values of colloidal and prosthetic phosphorus in yak casein micelles suggest that the yak casein is less phosphorylated than the cow one.
Bacterial Communities Found in Pit-Wall Mud and Factors Driving Their Evolution
Authors: Zhou Hao , Xu Boyang , Xu Shanshan , Jiang Suwei , Mu Dongdong , Wu Xuefeng , Li Xingjiang
Published: March 2023

Pit-wall mud (PWM) fosters bacterial communities involved in Baijiu production. PWM varies depending on pit age and height. In this study, we explored the bacterial communities in PWM and factors driving their evolution. The abundance and diversity of bacterial communities were low in new PWM (NPWM). In old PWM (OPWM), similar but diverse bacterial communities were observed at different heights. Lactobacillus was the predominant genus in NPWM, and Caproiciproducens, Aminobacterium, Hydrogenispora, Lactobacillus, Petrimonas, Syntrophomonas, and Sedimentibacter were the dominant genera in OPWM. A decrease was noted in the abundance of Lactobacillus, which indicated evolution. Among all the physicochemical properties, pH had the highest degree of interpretation with an R2 value of 0.965. pH also exerted the strongest effect on bacterial communities. The path coefficients of pH on bacterial community diversity and abundance were 0.886 and 0.810, respectively. Caproiciproducens and Clostridium sensu stricto 12 metabolized lactic acid, inhibiting the growth of Lactobacillus at a suitable pH, which led to the maturation of PWM. Our findings enrich the literature on the evolution of bacterial communities in PM and the maturation of PM.
Improvements in Visual Aspects and Chemical, Techno-Functional and Rheological Characteristics of Cricket Powder (Gryllus bimaculatus) by Solvent Treatment for Food Utilization
Authors: Damasceno Barbara Catalano , Nakajima Mitsutoshi , Taarji Noamane , Kobayashi Isao , Ichikawa Sosaku , Neves Marcos A.
Published: March 2023

This study aimed to improve the visual aspects and chemical, techno-functional and rheological characteristics of Gryllus bimaculatus cricket powder through the use of different solvents, with the objective of using it as a protein source in food production. Four treatments (pH 5 aqueous solution, ethanol 20%, ethanol 99.5%, and hexane) were applied to the powder, and analyses were conducted to assess changes in the previously mentioned parameters. The results showed that the treatments led to an increase in protein concentration (from 55.4 to 72.5%) and a decrease in fat concentration (from 33.0 to 6.8%) in ethanol 99.5% treated powder, as well as a reduction in anti-nutritional compounds concentration, such as tannins (from 13.3 to 5.9 g/kg), in pH 5 treated powder, which is important for the nutritional value of the final product. The color of the powders was improved, being lighter after hexane and ethanol 99.5% treatments due to the removal of melanin with the defatting process. Flowability, water, and oil holding capacity were also improved in the defatted powders. All the results suggest that the main composition of the powder directly influences the analyzed parameters. These findings suggest that cricket powder treated with solvents can be used as a protein source in different food applications.
The Use of Hydrogels for the Treatment of Bone Osteosarcoma via Localized Drug-Delivery and Tissue Regeneration: A Narrative Review
Authors: Tharakan Shebin , Raja Iman , Pietraru Annette , Sarecha Elina , Gresita Andrei , Petcu Eugen , Ilyas Azhar , Hadjiargyrou Michael
Published: March 2023

Osteosarcoma is a malignant tumor of bone that leads to poor mortality and morbidity. Management of this cancer through conventional methods involves invasive treatment options that place patients at an increased risk of adverse events. The use of hydrogels to target osteosarcoma has shown promising results both in vitro and in vivo to eradicate tumor cells while promoting bone regeneration. The loading of hydrogels with chemotherapeutic drugs provides a route for site-specific targeted therapy for osteosarcoma. Current studies demonstrate tumor regression in vivo and lysis of tumor cells in vitro when exposed to doped hydrogel scaffolds. Additionally, novel stimuli-responsive hydrogels are able to react with the tissue microenvironment to facilitate the controlled release of anti-tumor drugs and with biomechanical properties that can be modulated. This narrative review of the current literature discusses both in vitro and in vivo studies of different hydrogels, including stimuli-responsive, designed to treat bone osteosarcoma. Future applications to address patient treatment for this bone cancer are also discussed.
Mode of Action of Heat Shock Protein (HSP) Inhibitors against Viruses through Host HSP and Virus Interactions
Authors: Wu Shuang , Zhao Yongtian , Wang Delu , Chen Zhuo
Published: March 2023

Misfolded proteins after stress-induced denaturation can regain their functions through correct re-folding with the aid of molecular chaperones. As a molecular chaperone, heat shock proteins (HSPs) can help client proteins fold correctly. During viral infection, HSPs are involved with replication, movement, assembly, disassembly, subcellular localization, and transport of the virus via the formation of macromolecular protein complexes, such as the viral replicase complex. Recent studies have indicated that HSP inhibitors can inhibit viral replication by interfering with the interaction of the virus with the HSP. In this review, we describe the function and classification of HSPs, the transcriptional mechanism of HSPs promoted by heat shock factors (HSFs), discuss the interaction between HSPs and viruses, and the mode of action of HSP inhibitors at two aspects of inhibiting the expression of HSPs and targeting the HSPs, and elaborate their potential use as antiviral agents.
Genetic Diversity in Natural Populations of Rhodiola Species of Different Adaptation Strategies
Authors: Terletskaya Nina V. , Turzhanova Ainur S. , Khapilina Oxana N. , Zhumagul Moldir Z. , Meduntseva Nataliya D. , Kudrina Nataliya O. , Korbozova Nazym K. , Kubentayev Serik A. , Kalendar Ruslan
Published: March 2023

Representatives of the Crassulaceae family’s genus Rhodiola are succulents, making them distinctive in a changing environment. One of the most significant tools for analyzing plant resources, including numerous genetic processes in wild populations, is the analysis of molecular genetic polymorphism. This work aimed to look at the polymorphisms of allelic variations of the superoxide dismutase (SOD) and auxin response factor (ARF) gene families, as well as the genetic diversity of five Rhodiola species, using the retrotransposons-based fingerprinting approach. The multi-locus exon-primed intron-crossing (EPIC-PCR) profiling approach was used to examine allelic variations in the SOD and ARF gene families. We implemented the inter-primer binding site (iPBS) PCR amplification technique for genome profiling, which demonstrated a significant level of polymorphism in the Rhodiola samples studied. Natural populations of Rhodiola species have a great capacity for adaptation to unfavorable environmental influences. The genetic variety of wild populations of Rhodiola species leads to their improved tolerance of opposing environmental circumstances and species evolutionary divergence based on the diversity of reproductive systems.
An Attempt to Identify the Medaka Receptor for Somatolactin Alpha Using a Reverse Genetics Approach
Authors: Moroki Yuko , Komori Mamiko , Ogawa Yuko , Nagumo Erina , Ohno Haruna , Fukamachi Shoji
Published: March 2023

Somatolactin alpha (SLα) is a fish-specific hormone involved in body color regulation. The growth hormone (GH) is another hormone that is expressed in all vertebrates and promotes growth. These peptide hormones act by binding to receptors (SLα receptor (SLR) and GH receptor (GHR)); however, the relationships between these ligands and their receptors vary among species. Here, we first performed phylogenetic tree reconstruction by collecting the amino-acid sequences classified as SLR, GHR, or GHR-like from bony fish. Second, we impaired SLR or GHR functions in medaka (Oryzias sakaizumii) using CRISPR/Cas9. Lastly, we analyzed SLR and GHR mutants for phenotypes to deduce their functions. Phylogenetic tree reconstruction was performed using a total of 222 amino-acid sequences from 136 species, which revealed that many GHRa and GHRb are vaguely termed as GHR or GHR-like, while showing no orthologous/paralogous relationships. SLR and GHR mutants were successfully established for phenotyping. SLR mutants exhibited premature lethality after hatching, indicating an essential role for SLR in normal growth. GHR mutations did not affect viability, body length, or body color. These results provide no evidence that either SLR or GHR functions as a receptor for SLα; rather, phylogenetically and functionally, they seem to be receptors for GH, although their (subfunctionalized) roles warrant further investigation.
Mapping of QTLs Associated with Biological Nitrogen Fixation Traits in Peanuts (Arachis hypogaea L.) Using an Interspecific Population Derived from the Cross between the Cultivated Species and Its Wild Ancestors
Authors: Nzepang Darius T. , Gully Djamel , Nguepjop Joël R. , Zaiya Zazou Arlette , Tossim Hodo-Abalo , Sambou Aissatou , Rami Jean-François , Hocher Valerie , Fall Saliou , Svistoonoff Sergio , Fonceka Daniel
Published: March 2023

Peanuts (Arachis hypogaea L.) are an allotetraploid grain legume mainly cultivated by poor farmers in Africa, in degraded soil and with low input systems. Further understanding nodulation genetic mechanisms could be a relevant option to facilitate the improvement of yield and lift up soil without synthetic fertilizers. We used a subset of 83 chromosome segment substitution lines (CSSLs) derived from the cross between a wild synthetic tetraploid AiAd (Arachis ipaensis × Arachis duranensis)4× and the cultivated variety Fleur11, and evaluated them for traits related to BNF under shade-house conditions. Three treatments were tested: without nitrogen; with nitrogen; and without nitrogen, but with added0 Bradyrhizobium vignae strain ISRA400. The leaf chlorophyll content and total biomass were used as surrogate traits for BNF. We found significant variations for both traits specially linked to BNF, and four QTLs (quantitative trait loci) were consistently mapped. At all QTLs, the wild alleles decreased the value of the trait, indicating a negative effect on BNF. A detailed characterization of the lines carrying those QTLs in controlled conditions showed that the QTLs affected the nitrogen fixation efficiency, nodule colonization, and development. Our results provide new insights into peanut nodulation mechanisms and could be used to target BNF traits in peanut breeding programs.
Inflammation and Oxidative Stress Gene Variability in Retinal Detachment Patients with and without Proliferative Vitreoretinopathy
Authors: Lumi Xhevat , Confalonieri Filippo , Ravnik-Glavač Metka , Goričar Katja , Blagus Tanja , Dolžan Vita , Petrovski Goran , Hawlina Marko , Glavač Damjan
Published: March 2023

This study investigated the association between certain genetic variations and the risk of developing proliferative vitreoretinopathy (PVR) after surgery. The study was conducted on 192 patients with primary rhegmatogenous retinal detachment (RRD) who underwent 3-port pars plana vitrectomy (PPV). The distribution of single nucleotide polymorphisms (SNPs) located in genes involved in inflammation and oxidative stress associated with PVR pathways were analyzed among patients with and without postoperative PVR grade C1 or higher. A total of 7 defined SNPs of 5 genes were selected for genotyping: rs4880 (SOD2); rs1001179 (CAT); rs1050450 (GPX1); rs1143623, rs16944, rs1071676 (IL1B); rs2910164 (MIR146A) using competitive allele-specific polymerase chain reaction. The association of SNPs with PVR risk was evaluated using logistic regression. Furthermore, the possible association of SNPs with postoperative clinical parameters was evaluated using non-parametric tests. The difference between two genotype frequencies between patients with or without PVR grade C1 or higher was found to be statistically significant: SOD2 rs4880 and IL1B rs1071676. Carriers of at least one polymorphic IL1B rs1071676 GG allele appeared to have better postoperative best-corrected visual acuity only in patients without PVR (p = 0.070). Our study suggests that certain genetic variations may play a role in the development of PVR after surgery. These findings may have important implications for identifying patients at higher risk for PVR and developing new treatments.
Implementation of Surrogate Models for the Analysis of Slope Problems
Authors: Mitelman Amichai , Yang Beverly , Elmo Davide
Published: March 2023

Numerical modeling is increasingly used to analyze practical rock engineering problems. The geological strength index (GSI) is a critical input for many rock engineering problems. However, no available method allows the quantification of GSI input parameters, and engineers must consider a range of values. As projects progress, these ranges can be narrowed down. Machine learning (ML) algorithms have been coupled with numerical modeling to create surrogate models. The concept of surrogate models aligns well with the deductive nature of data availability in rock engineering projects. In this paper, we demonstrated the use of surrogate models to analyze two common rock slope stability problems: (1) determining the maximum stable depth of a vertical excavation and (2) determining the allowable angle of a slope with a fixed height. Compared with support vector machines and K-nearest algorithms, the random forest model performs best on a data set of 800 numerical models for the problems discussed in the paper. For all these models, regression-type models outperform classification models. Once the surrogate model is confirmed to preform accurately, instantaneous predictions of maximum excavation depth and slope angle can be achieved according to any range of input parameters. This capability is used to investigate the impact of narrowing GSI range estimation.
Analysing the Large-Scale Debris Flow Event in July 2022 in Horlachtal, Austria Using Remote Sensing and Measurement Data
Authors: Rom Jakob , Haas Florian , Hofmeister Florentin , Fleischer Fabian , Altmann Moritz , Pfeiffer Madlene , Heckmann Tobias , Becht Michael
Published: March 2023

High-quality in situ measurements are essential for hazard assessment of debris flow events. However, precise data on debris flow triggering thresholds, accumulation volumes and spatial characteristics of large-scale events on catchment scale are scarce due to the rare occurrence of debris flows and the challenges of acquiring accurate data for a larger area. In this study, we present quantitative analyses of a single extreme debris flow event in the Horlachtal, Austria, triggered by local high-intensity short-duration precipitation events on 20 and 23 July 2022. Pre- and post-event airborne LiDAR (light detection and ranging) data with a high spatial resolution reveal that 156 different debris flow processes were initiated during these events, with accumulation volumes of up to approximately 40,000 m³. The calculated debris flow deposition volumes also show a power-law relationship with the total amount of rainfall in the respective debris flow catchments. The spatial appearance of the debris flows shows a concentration of processes in a particular area rather than a uniform distribution, suggesting a local nature of the triggering event. This is further supported by the measurements from three meteorological stations and four discharge gauges within the study area. The gridded area-wide INCA (Integrated Nowcasting through Comprehensive Analysis) rainfall data further point to a local convective event on 20 July 2022, with a maximum rainfall intensity of 44 mm/h.
Yield Response and Antioxidant Activity of Greenhouse Organic Pumpkin (Cucurbita moschata Duch.) as Affected by Soil Solarization and Biofumigation
Authors: Castronuovo Donato , De Feo Vincenzo , De Martino Laura , Cardone Loriana , Sica Rita , Caputo Lucia , Amato Giuseppe , Candido Vincenzo
Published: March 2023

In intensive cropping systems, soil fumigation, i.e., the use of gas chemical pesticides applied to the soil covered with impermeable plastic film, represents an effective method to control most of the soilborne pests. However, its general non-selectivity to the useful soil microflora and microfauna together with their significant environmental problems has limited their use. Numerous studies have concerned the application of less impactful but, at the same time, equally effective disinfection methods. These are techniques with a low environmental impact that are particularly suitable for horticultural systems. Among these, both soil solarization and biofumigation are popular techniques, even in organic vegetable cropping systems. This paper reports the results of a study to evaluate the effects of soil solarization on the yield response and the antioxidant levels of organic pumpkin (Cucurbita moscata Duch.). Solarization was carried out alone and with the addition of some organic matrices, such as compost, manure and green manure species. The evaluation of the antioxidant activity was carried out with DPPH (1,1-diphenyl-2-picrylhydrazyl radical) assay: DPPH has a specific absorption band, which disappears in presence of an antiradical compound, showing its antioxidant capacity. Our results revealed that the pumpkins grown in solarized soil had IC50, i.e., the concentration of sample which reduced the initial DPPH of 50%, values ranging between 0.6 to 18.0 mg mL−1, lower than the values of IC50 of pumpkins grown in non-solarized soil, which ranged from 36.0 to 43.6 mg mL−1. The obtained results highlighted the validity and utility of solarization for the organic vegetable crops. Furthermore, this technique has also shown its effectiveness in the long term since yield increases concerned several crops in succession. Finally, our results highlighted that heat treatment solarization can influence the level of several compounds in the pumpkin, and, therefore, also of the polysaccharides.
Functional Characterization of Ammonium Transporter MhAMT1;2 in Malus hupehensis
Authors: Li Jiazhen , Yu Muting , Li Huibin , Yang Guangkai , Huang Linlin , Hao Yanyan
Published: March 2023

The absorption and utilization of NH4+ and NO3− by plant roots is closely related to soil moisture. In this study, we investigated the effect of short-time drought and rewatering on uptake and assimilation of NH4+ and NO3− in 1-year-old Malus hupehensis plants, as well as transcription changes of ammonium transporters (AMTs) and nitrate transporters (NRTs). In roots, the NH4+ and NO3− content and nitrate reductase activity decreased under drought and to some extent was restored after rewatering. Expression analysis indicated that most investigated AMTs and NRTs were down-regulated while MhAMT1;2 was significantly up-regulated in drought-stressed roots. Therefore, the function of MhAMT1;2 was further studied through bioinformatics analysis, tissue-specific expression, subcellular localization, and functional complementation in NH4+ uptake-defective yeast and Arabidopsis mutants. Results showed that MhAMT1;2 was mainly expressed in roots and localized to the cell membrane. Moreover, MhAMT1;2 can mediate NH4+ uptake in both yeast and Arabidopsis mutants, and the transport process was affected by external proton concentrations and ATP. The present study will create a basis for exploring the functional roles of plant AMT members and improving N uptake and use efficiency under drought condition in fruit trees.
Influence of Provider and Leader Perspectives about Concurrent Tobacco-Use Care during Substance-Use Treatment on Their Tobacco Intervention Provision with Clients: A Mixed-Methods Study
Authors: Britton Maggie , Martinez Leal Isabel , Jafry Midhat Z. , Chen Tzuan A. , Rogova Anastasia , Kyburz Bryce , Williams Teresa , Reitzel Lorraine R.
Published: March 2023

People with substance-use disorders have elevated rates of tobacco use compared with the general population, yet rarely receive tobacco-dependence treatment within substance-use treatment settings (SUTS). One barrier to delivering evidence-based interventions in SUTS is providers’ misconception that treating tobacco use and non-nicotine substance use concurrently jeopardizes clients’ substance-use recovery, although research indicates that it enhances support for recovery and relapse prevention. A total of 86 treatment providers employed in SUTS (i.e., 9 Federally Qualified Health Centers, 16 Local Mental Health Authorities (LMHAs), 6 substance-use treatment programs in LMHAs, and 55 stand-alone substance-use treatment centers) in Texas, USA, answered survey questions about their (1) thoughts about treating tobacco during substance-use treatment, and (2) delivery of the 5A’s tobacco-use intervention (Ask, Advise, Assess, Assist, Arrange). Twenty-six providers and leaders were interviewed about attitudes toward tobacco-free workplace policies and tobacco dependence and the relative importance of treating tobacco (vs. other substance-use disorders) at their center. Providers who did not believe tobacco use should be addressed as soon as clients begin treatment (i.e., endorsed responses of after 1 year, it depends on the client, or never) had lower odds of Asking clients about their tobacco use (OR = 0.195), Advising clients to quit smoking (OR = 0.176), and Assessing interest in quitting smoking (OR = 0.322). Qualitative results revealed barriers including beliefs that clients need to smoke to relieve the stress of substance-use recovery, are disinterested in quitting, fears that concurrent treatment would jeopardize substance use, and limited resources; additional training and education resources was the key facilitator theme. The results demonstrate a critical need to eliminate barriers to tobacco-treatment provision for clients in SUTS through education to correct misperceptions, specialized training to equip providers with knowledge and skills, and resources to build center capacity. Integrating evidence-based smoking interventions into routine care is key to support the recovery efforts of clients in SUTS.
Trends in Tobacco Use among 9th Graders in Sweden, 1991–2020
Authors: Raninen Jonas , Gripe Isabella , Zetterqvist Martina , Ramstedt Mats
Published: March 2023

Tobacco use was measured with self-reports of lifetime use of cigarettes and snus to examine trends in tobacco use among Swedish 9th graders over the period 1991–2020. Annual school surveys with nationally representative samples of 9th-grade students in Sweden covering the period 1991–2020 with a total sample of 163,617 students. We distinguished between the use of cigarettes only, use of snus only, dual use (use of both cigarettes and snus), and total tobacco use (use of any of these tobacco products). In addition to a graphical description of trends in the various measures of tobacco use, the correlation between these trends was calculated with the Pearson correlation coefficient (Rxy). The prevalence of total tobacco use declined from 72% in 1991 to 36% in 2020. The declining trend in total tobacco use was positively correlated with the trend in dual use (Rxy = 0.98) and the trend in cigarette use only (Rxy = 0.87). The trend in total tobacco use was, on the other hand, negatively correlated with snus use only (Rxy = −0.41), and snus use only was negatively correlated with cigarette use only (Rxy = −0.71). The situation became different after 2017 when total tobacco use increased as a result of an increasing prevalence of snus use. The sharp decline in tobacco use among 9th graders in Sweden over the past three decades is driven by declining cigarette use. The correlations between the various forms of tobacco use suggest that snus use may have contributed to the decline in cigarette use and, by that, overall tobacco use. The situation changed after 2017 when a sharp rise in snus use seems to have increased total tobacco use among adolescents in Sweden. A possible explanation behind this development is the introduction of a new form of snus called “All white snus”, which was introduced in Sweden in 2014.
Unintentional Injuries Are Associated with Self-Reported Child Maltreatment among Swedish Adolescents
Authors: Tindberg Ylva , Janson Staffan , Jernbro Carolina
Published: March 2023

Injuries constitute a large share of childhood morbidity and mortality. This study examines whether adolescents with self-reported experiences of different types of child maltreatment more frequently reported unintentional injury events requiring health- or dental care during the last year and/or hospitalization at any time during childhood. Cross-sectional data from a Swedish national representative school survey (2016) including 4741 adolescents were used (78.5% response rate). Data were analyzed with univariate tests and multiple logistic regression. Statistically significant associations between reported experiences of physical, psychological and sexual abuse, neglect, and witnessing partner violence during childhood and reported unintentional injuries requiring health- or dental care during the last year were found (aOR:s between 1.39–1.77). The corresponding association for poly-victimization was aOR 1.91 (95% CI 1.39–2.62). Furthermore, a linear-by-linear association was seen for degree of victimization and number of episodes of unintentional injuries that required care in the last year (p = 0.000), as well as lifetime hospitalizations (p = 0.000). This study shows significant associations between child maltreatment and unintentional injuries requiring health- and/or dental care and hospitalization. To improve both injury and child maltreatment prevention, healthcare professionals need to pay particular attention to children and adolescents who repeatedly seek healthcare services due to injurious events.
“Just Standing Still”: A Qualitative Study on Adolescents’ Experiences of School Closures Due to Emerging COVID-19 in Bissau, Guinea-Bissau
Authors: N’dure Baboudóttir Fatou , Jandi Zeca , Indjai Bucar , Einarsdóttir Jónína , Gunnlaugsson Geir
Published: March 2023

The COVID-19 pandemic affected the lives of children in a myriad of ways across the world. It exposed and aggravated existing inequalities between children within countries and across continents and hampered education. In Guinea-Bissau, school closure was one of the first restrictions implemented to confront the emerging pandemic. The aim was to describe and analyse the experiences of adolescents of school closures in the capital Bissau, their concerns about their future and manifestations of inequality. Data were collected by semi-structured, open-ended interviews with 30 adolescents aged 15–17 years three months into the pandemic during an enforced state of emergency. A thematic analysis identified five themes: appreciation of education, feeling left behind, being stuck in confinement, suggestions for support, and a disrupted future. The results highlight global rather than local inequalities in the demographic, manifested by a lack of targeted educational support for public and private school students; they knew about such efforts elsewhere. The school-attending participants suggested ways to mitigate disruptions in their education, while those out of school aiming to return saw their possibilities fading away. They appreciated education for personal and national benefits, and participants worried about the long-term effects of the pandemic. The study highlighted education loss for all and disrupted future expectations.
Analyzing and Validating a Structure for Measuring the Nurse Practice Environment
Authors: Rodwell John , Hendry Thomas , Johnson Dianne
Published: March 2023

Nurse shortages pose a challenge in many countries and retaining existing nursing staff is crucial to addressing these shortages. To inform possible interventions aimed at retaining nurses, managers need a comprehensive understanding of the nature of the nurse practice environment. The scales from two of the main instruments used to assess nurses’ practice environments are tested. A survey of an online panel obtained responses from 459 Australian nurses. Analyses determined a combination of items with good construct validity and improved predictive utility for outcomes of interest for individual nurses. By essentially combining the best items from each instrument, a more comprehensive representation of the nurse work environment is obtained with improved predictive utility. The resulting combined set of scales is recommended for analyses of the nurse working environment and uses a combined set of scales from each of the two source instruments, namely: nurse participation in hospital affairs, recognition, nursing process, peer work standards, nursing competence, orientation, managers, resources, nurse–physician collaboration, and positive scheduling climate. Future research can then build on that strong set of items with a validated structure and predictive utility to inform management and interventions.
Biomonitoring California Protocol for Following up on Elevated Levels of Urinary Arsenic
Authors: Iyer Shoba , Kauffman Duyen , Steinmaus Craig , Hoover Sara
Published: March 2023

Objectives: to develop and implement a follow-up protocol for Biomonitoring California study participants with elevated levels of urinary arsenic, particularly inorganic forms. Methods: We selected 20 μg/L as the level of concern for urinary inorganic arsenic; samples with total arsenic ≥20 μg/L were speciated. Participants with elevated inorganic arsenic were notified of their level and invited to participate in a telephone survey to help determine possible exposure sources. We illustrate the protocol in four Biomonitoring California studies, which collected samples from 2010–2013 in locations across the state. Results: 48 participants in the four studies had elevated urinary inorganic arsenic levels. Consumption of rice and rice-based products was the most commonly identified potential source of inorganic arsenic exposure. Conclusions: Of 48 participants with elevated inorganic arsenic, 27 would have been missed if we had used the previously published threshold of 50 µg/L total arsenic to identify urine samples for speciation. This protocol fills a gap in the clinical literature by providing a more health-protective approach to identify individuals with elevated urinary inorganic arsenic and help determine potentially significant exposure sources.
Analysis of the Spatiotemporal Urban Expansion of the Rome Coastline through GEE and RF Algorithm, Using Landsat Imagery
Authors: Lodato Francesco , Colonna Nicola , Pennazza Giorgio , Praticò Salvatore , Santonico Marco , Vollero Luca , Pollino Maurizio
Published: March 2023

This study analyzes, through remote sensing techniques and innovative clouding services, the recent land use dynamics in the North-Roman littoral zone, an area where the latest development has witnessed an important reconversion of purely rural areas to new residential and commercial services. The survey area includes five municipalities and encompasses important infrastructure, such as the “Leonardo Da Vinci” Airport and the harbor of Civitavecchia. The proximity to the metropolis, supported by an efficient network of connections, has modified the urban and peri-urban structure of these areas, which were formerly exclusively agricultural. Hereby, urban expansion has been quantified by classifying Landsat satellite images using the cloud computing platform “Google Earth Engine” (GEE). Landsat multispectral images from 1985 up to 2020 were used for the diachronic analysis, with a five-yearly interval. In order to achieve a high accuracy of the final result, work was carried out along the temporal dimension of the images, selecting specific time windows for the creation of datasets, which were adjusted by the information related to the NDVI index variation through time. This implementation showed interesting improvements in the model performance for each year, suggesting the importance of the NDVI standard deviation parameter. The results showed an increase in the overall accuracy, being from 90 to 97%, with improvements in distinguishing urban surfaces from impervious surfaces. The final results highlighted a significant increase in the study area of the “Urban” and “Woodland” classes over the 35-year time span that was considered, being 67.4 km2 and 70.4 km2, respectively. The accurate obtained results have allowed us to quantify and understand the landscape transformations in the area of interest, with particular reference to the dynamics of urban development.
Comprehensive Analysis of the INDETERMINATE DOMAIN (IDD) Gene Family and Their Response to Abiotic Stress in Zea mays
Authors: Feng Xue , Yu Qian , Zeng Jianbin , He Xiaoyan , Ma Wujun , Ge Lei , Liu Wenxing
Published: March 2023

Transcription factors (TFs) are important regulators of numerous gene expressions due to their ability to recognize and combine cis-elements in the promoters of target genes. The INDETERMINATE DOMAIN (IDD) gene family belongs to a subfamily of C2H2 zinc finger proteins and has been identified only in terrestrial plants. Nevertheless, little study has been reported concerning the genome-wide analysis of the IDD gene family in maize. In total, 22 ZmIDD genes were identified, which can be distributed on 8 chromosomes in maize. On the basis of evolutionary relationships and conserved motif analysis, ZmIDDs were categorized into three clades (1, 2, and 3), each owning 4, 6, and 12 genes, respectively. We analyzed the characteristics of gene structure and found that 3 of the 22 ZmIDD genes do not contain an intron. Cis-element analysis of the ZmIDD promoter showed that most ZmIDD genes possessed at least one ABRE or MBS cis-element, and some ZmIDD genes owned the AuxRR-core, TCA-element, TC-rich repeats, and LTR cis-element. The Ka:Ks ratio of eight segmentally duplicated gene pairs demonstrated that the ZmIDD gene families had undergone a purifying selection. Then, the transcription levels of ZmIDDs were analyzed, and they showed great differences in diverse tissues as well as abiotic stresses. Furthermore, regulatory networks were constructed through the prediction of ZmIDD-targeted genes and miRNAs, which can inhibit the transcription of ZmIDDs. In total, 6 ZmIDDs and 22 miRNAs were discovered, which can target 180 genes and depress the expression of 9 ZmIDDs, respectively. Taken together, the results give us valuable information for studying the function of ZmIDDs involved in plant development and climate resilience in maize.
Circulating microRNA as Biomarkers for Gestational Diabetes Mellitus—A Systematic Review and Meta-Analysis
Authors: Dinesen Sofie , El-Faitarouni Alisar , Frisk Nanna Lond Skov , Sørensen Anja Elaine , Dalgaard Louise Torp
Published: March 2023

Gestational diabetes mellitus (GDM) is a severe pregnancy complication for both the woman and the child. Women who suffer from GDM have a greater risk of developing Type 2 diabetes mellitus (T2DM) later in life. Identification of any potential biomarkers for the early prediction of gestational diabetes can help prevent the disease in women with a high risk. Studies show microRNA (miRNA) as a potential biomarker for the early discovery of GDM, but there is a lack of clarity as to which miRNAs are consistently altered in GDM. This study aimed to perform a systematic review and meta-analysis to investigate miRNAs associated with GDM by comparing GDM cases with normoglycemic controls. The systematic review was performed according to PRISMA guidelines with searches in PubMed, Web of Science, and ScienceDirect. The primary search resulted in a total of 849 articles, which were screened according to the prior established inclusion and exclusion criteria. Following the screening of articles, the review was based on the inclusion of 35 full-text articles, which were evaluated for risk of bias and estimates of quality, after which data were extracted and relative values for miRNAs were calculated. A meta-analysis was performed for the miRNA species investigated in three or more studies: MiR-29a, miR-330, miR-134, miR-132, miR-16, miR-223, miR-155, miR-122, miR-17, miR-103, miR-125, miR-210, and miR-222. While some miRNAs showed considerable between-study variability, miR-29a, miR-330, miR-134, miR-16, miR-223, and miR-17 showed significant overall upregulation in GDM, while circulating levels of miR-132 and miR-155 were decreased among GDM patients, suggesting further studies of these as biomarkers for early GDM discovery.
Micromechanical Study of Hyperacetylated Nucleosomes Using Single Molecule Transverse Magnetic Tweezers
Authors: Gaire Santosh , Fabian Roberto L. , Adhikari Raghabendra , Tuma Pamela L. , Pegg Ian L. , Sarkar Abhijit
Published: March 2023

Nucleosomes are stable complexes of DNA and histone proteins that are essential for the proper functioning of the genome. These structures must be unwrapped and disassembled for processes such as gene expression, replication, and repair. Histone post-translational modifications (PTMs) are known to play a significant role in regulating the structural changes of nucleosomes. However, the underlying mechanisms by which these modifications function remain unclear. In this study, we report the results of single molecule micromanipulation experiments on DNA–protein complexes composed of hyperacetylated histone proteins using transverse magnetic tweezers. The experiments were conducted by pre-extending λ-DNA with a force less than 4 pN before introducing hyperacetylated histones into the sample chamber. The DNA shortened as the histones formed complexes with it and the nucleosome arrays were then exposed to increasing tension, resulting in quantized changes in the DNA’s extension with step sizes of (integral multiples of) ~50 nm. We also compared results of experiments using PTM histones and native histones with data collected for both types of histones for the same force ranges (2–80 pN) and loading rates. Our data show that hyperacetylated nucleosomes require an unbinding force of around ~2.5 pN, which is similar to that required for native histones. Moreover, we identified clear differences between the step-size distributions of native and hyperacetylated histones and found that in contrast to tethers reconstituted with native histones, the majority of nucleosomes in tethers compacted with hyperacetylated histones underwent disassembly at forces significantly lower than 6 pN.
Experimental Studies on the Thermal Properties and Decomposition Course of a Novel Class of Heterocyclic Anticancer Drug Candidates
Authors: Worzakowska Marta , Sztanke Małgorzata , Sztanke Krzysztof
Published: March 2023

The experimental studies on the thermal properties and decomposition course of a novel class of potential anticancer drugs (1–5) containing in their heterobicyclic structures the asymmetrical triazine template were performed with the use of differential scanning calorimetry (DSC) and simultaneous thermogravimetry/differential scanning calorimetry (TG/DTG/DSC) coupled online with Fourier transform infrared spectroscopy (FTIR) and quadrupole mass spectrometry (QMS) in inert and oxidizing conditions. All the compounds were thermally characterized in detail for the first time in this article. The DSC studies proved that the melting points of the tested compounds depended on the position and type of the substituent at the phenyl moiety, whereas they did not depend on the furnace atmosphere. All the tested polynitrogenated heterocycles proved to be molecules with high thermal stability in both atmospheres, and most of them (1, 3–5) were more stable in oxidizing conditions, which indicated the formation of a more thermally stable form of the compounds when interacting with oxygen. The simultaneous TG/FTIR/QMS analyses confirmed that their pyrolysis process occurred in one main stage resulting in the emission of volatiles such as NH3, HNCO, HCN, CO, CO2, H2O, NO2, aromatic amine derivatives, alkenes (for compounds 1–5), and HCl (for the compound 5). On the other hand, the oxidative decomposition process was more complicated and proceeded in two main stages leading to the emission of NH3, CO2, CO, HCN, HNCO, H2O, some aromatics (for compounds 1–5), HCl (for compounds 3–5) as well as the additional volatiles such as N2, NO2, NH2OH, and (CN)2. The type of the formed volatiles indicated that the decomposition process of the studied heterocycles under the influence of heating was initiated by the radical mechanism. Their decomposition was related to the symmetric cleavage of C–N and C–C bonds (inert conditions) and additional reaction of the volatiles and residues with oxygen (oxidizing conditions).