bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential Cross Talk between Autism Risk Genes and Neurovascular Molecules: A Pilot Study on Impact of Blood Brain Barrier Integrity
Authors: Jagadapillai Rekha , Qiu Xiaolu , Ojha Kshama , Li Zhu , El-Baz Ayman , Zou Shipu , Gozal Evelyne , Barnes Gregory N.
Published: July 2022

Autism Spectrum Disorder (ASD) is a common pediatric neurobiological disorder with up to 80% of genetic etiologies. Systems biology approaches may make it possible to test novel therapeutic strategies targeting molecular pathways to alleviate ASD symptoms. A clinical database of autism subjects was queried for individuals with a copy number variation (CNV) on microarray, Vineland, and Parent Concern Questionnaire scores. Pathway analyses of genes from pathogenic CNVs yielded 659 genes whose protein–protein interactions and mRNA expression mapped 121 genes with maximal antenatal expression in 12 brain regions. A Research Domain Criteria (RDoC)-derived neural circuits map revealed significant differences in anxiety, motor, and activities of daily living skills scores between altered CNV genes and normal microarrays subjects, involving Positive Valence (reward), Cognition (IQ), and Social Processes. Vascular signaling was identified as a biological process that may influence these neural circuits. Neuroinflammation, microglial activation, iNOS and 3-nitrotyrosine increase in the brain of Semaphorin 3F- Neuropilin 2 (Sema 3F-NRP2) KO, an ASD mouse model, agree with previous reports in the brain of ASD individuals. Signs of platelet deposition, activation, release of serotonin, and albumin leakage in ASD-relevant brain regions suggest possible blood brain barrier (BBB) deficits. Disruption of neurovascular signaling and BBB with neuroinflammation may mediate causative pathophysiology in some ASD subgroups. Although preliminary, these data demonstrate the potential for developing novel therapeutic strategies based on clinically derived data, genomics, cognitive neuroscience, and basic neuroscience methods.
Organ-Specific Glucose Uptake: Does Sex Matter?
Authors: Gandhi Adithi , Tang Ryan , Seo Youngho , Bhargava Aditi
Published: July 2022

Glucose uptake by peripheral organs is essential for maintaining blood glucose levels within normal range. Impaired glucose uptake is a hallmark of type 2 diabetes (T2D) and metabolic syndrome and is characterized by insulin resistance. Male sex is an independent risk factor for the development of T2D. We tested whether sex and diet are independent variables for differential glucose uptake by various organs. Here, in a longitudinal study, we used 18F-fluorodeoxyglucose (FDG) and positron emission tomography (PET) to determine baseline differences in whole-body glucose uptake in young male and female mice on chow and high-fat diets. We report that sex and diet are important independent variables that account for differential glucose uptake in brown fat, skeletal muscle, liver, heart, kidney, and the stomach, but not the brain, lungs, pancreas, small intestine, or perigonadal adipose. Of the seven organs analyzed, two organs, namely brown fat, and the heart had the highest concentrations of FDG, followed by the brain, kidneys, and skeletal muscle on chow diet. Young female mice had 47% greater FDG uptake in the brown fat compared to male mice, whereas skeletal muscle FDG uptake was 49% greater in male mice. The high-fat diet inhibited FDG uptake in brown fat, skeletal muscle, and the heart, three major organs involved in uptake, whereas brain uptake was enhanced in both sexes. These foundational and groundbreaking findings suggest that mechanisms of glucose homeostasis are context- and organ-dependent and highlight the need to study sex-specific outcomes and mechanisms for diseases such as T2D, obesity, and metabolic syndrome.
Immunocytochemical Analysis of the Wall Ingrowths in the Digestive Gland Transfer Cells in Aldrovanda vesiculosa L. (Droseraceae)
Authors: Płachno Bartosz J. , Kapusta Małgorzata , Stolarczyk Piotr , Świątek Piotr , Strzemski Maciej , Miranda Vitor F. O.
Published: July 2022

Carnivorous plants are unique due to their ability to attract small animals or protozoa, retain them in specialized traps, digest them, and absorb nutrients from the dissolved prey material; however, to this end, these plants need a special secretion-digestive system (glands). A common trait of the digestive glands of carnivorous plants is the presence of transfer cells. Using the aquatic carnivorous species Aldrovanda vesiculosa, we showed carnivorous plants as a model for studies of wall ingrowths/transfer cells. We addressed the following questions: Is the cell wall ingrowth composition the same between carnivorous plant glands and other plant system models? Is there a difference in the cell wall ingrowth composition between various types of gland cells (glandular versus endodermoid cells)? Fluorescence microscopy and immunogold electron microscopy were employed to localize carbohydrate epitopes associated with major cell wall polysaccharides and glycoproteins. The cell wall ingrowths were enriched with arabinogalactan proteins (AGPs) localized with the JIM8, JIM13, and JIM14 epitopes. Both methylesterified and de-esterified homogalacturonans (HGs) were absent or weakly present in the wall ingrowths in transfer cells (stalk cells and head cells of the gland). Both the cell walls and the cell wall ingrowths in the transfer cells were rich in hemicelluloses: xyloglucan (LM15) and galactoxyloglucan (LM25). There were differences in the composition between the cell wall ingrowths and the primary cell walls in A. vesiculosa secretory gland cells in the case of the absence or inaccessibility of pectins (JIM5, LM19, JIM7, LM5, LM6 epitopes); thus, the wall ingrowths are specific cell wall microdomains. Even in the same organ (gland), transfer cells may differ in the composition of the cell wall ingrowths (glandular versus endodermoid cells). We found both similarities and differences in the composition of the cell wall ingrowths between the A. vesiculosa transfer cells and transfer cells of other plant species.
Interplay between Lipid Metabolism, Lipid Droplets, and DNA Virus Infections
Authors: Farías Mónica A. , Diethelm-Varela Benjamín , Navarro Areli J. , Kalergis Alexis M. , González Pablo A.
Published: July 2022

Lipid droplets (LDs) are cellular organelles rich in neutral lipids such as triglycerides and cholesterol esters that are coated by a phospholipid monolayer and associated proteins. LDs are known to play important roles in the storage and availability of lipids in the cell and to serve as a source of energy reserve for the cell. However, these structures have also been related to oxidative stress, reticular stress responses, and reduced antigen presentation to T cells. Importantly, LDs are also known to modulate viral infection by participating in virus replication and assembly. Here, we review and discuss the interplay between neutral lipid metabolism and LDs in the replication cycle of different DNA viruses, identifying potentially new molecular targets for the treatment of viral infections.
Transcriptional Regulation of the Hippo Pathway: Current Understanding and Insights from Single-Cell Technologies
Authors: Paul Sayantanee , Xie Shiqi , Yao Xiaosai , Dey Anwesha
Published: July 2022

The Hippo pathway regulates tissue homeostasis in normal development and drives oncogenic processes. In this review, we extensively discuss how YAP/TAZ/TEAD cooperate with other master transcription factors and epigenetic cofactors to orchestrate a broad spectrum of transcriptional responses. Even though these responses are often context- and lineage-specific, we do not have a good understanding of how such precise and specific transcriptional control is achieved—whether they are driven by differences in TEAD paralogs, or recruitment of cofactors to tissue-specific enhancers. We believe that emerging single-cell technologies would enable a granular understanding of how the Hippo pathway influences cell fate and drives oncogenic processes, ultimately allowing us to design better pharmacological agents against TEADs and identify robust pharmacodynamics markers of Hippo pathway inhibition.
Bronchoalveolar-Lavage-Derived Fibroblast Cell Lines Provide Tools for Investigating Various Interstitial Lung Diseases
Authors: Lehtonen Siri , Kaarteenaho Riitta
Published: July 2022

Bronchoalveolar lavage (BAL) is an important diagnostic and research tool for the investigation of various lung diseases. In addition to inflammatory and epithelial cells, BAL fluid may contain a small number of stromal cells, such as fibroblasts. During the past 30 years, a number of research groups have cultured BAL-derived fibroblasts for several passages in vitro. In addition to fibroblasts, these cultures have been reported to contain fibrocytes, myofibroblasts, and stem cells. We aim to present a summary of studies that have cultured stromal cells from BAL fluid.
Diversification of Potassium Currents in Excitable Cells via Kvβ Proteins
Authors: Dwenger Marc M. , Raph Sean M. , Baba Shahid P. , Moore Joseph B. , Nystoriak Matthew A.
Published: July 2022

Excitable cells of the nervous and cardiovascular systems depend on an assortment of plasmalemmal potassium channels to control diverse cellular functions. Voltage-gated potassium (Kv) channels are central to the feedback control of membrane excitability in these processes due to their activation by depolarized membrane potentials permitting K+ efflux. Accordingly, Kv currents are differentially controlled not only by numerous cellular signaling paradigms that influence channel abundance and shape voltage sensitivity, but also by heteromeric configurations of channel complexes. In this context, we discuss the current knowledge related to how intracellular Kvβ proteins interacting with pore complexes of Shaker-related Kv1 channels may establish a modifiable link between excitability and metabolic state. Past studies in heterologous systems have indicated roles for Kvβ proteins in regulating channel stability, trafficking, subcellular targeting, and gating. More recent works identifying potential in vivo physiologic roles are considered in light of these earlier studies and key gaps in knowledge to be addressed by future research are described.
MicroRNAs and Long Non-Coding RNAs in Adrenocortical Carcinoma
Authors: Detomas Mario , Pivonello Claudia , Pellegrini Bianca , Landwehr Laura-Sophie , Sbiera Silviu , Pivonello Rosario , Ronchi Cristina L. , Colao Annamaria , Altieri Barbara , De Martino Maria Cristina
Published: July 2022

Non-coding RNAs (ncRNAs) are a type of genetic material that do not encode proteins but regulate the gene expression at an epigenetic level, such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs). The role played by ncRNAs in many physiological and pathological processes has gained attention during the last few decades, as they might be useful in the diagnosis, treatment and management of several human disorders, including endocrine and oncological diseases. Adrenocortical carcinoma (ACC) is a rare and aggressive endocrine cancer, still characterized by high mortality and morbidity due to both endocrine and oncological complications. Despite the rarity of this disease, recently, the role of ncRNA has been quite extensively evaluated in ACC. In order to better explore the role of the ncRNA in human ACC, this review summarizes the current knowledge on ncRNA dysregulation in ACC and its potential role in the diagnosis, treatment, and management of this tumor.
Development and Optimization of Electrochemical Method for Determination of Vitamin C
Authors: Škugor Rončević Ivana , Skroza Danijela , Vrca Ivana , Kondža Ana Marija , Vladislavić Nives
Published: July 2022

The focus of this work was to develop a simple electrochemical method for the determination of vitamin C (VitC) by using a specially constructed microelectrode made from pyrolytic graphite sheet (PGS). A procedure for quantifying VitC in a real sample was established. VitC shows a single quasi-reversible reaction. The method was optimized, and analytical determination was performed by using cyclic voltammetry and square wave voltammetry for electroanalytical purposes. The obtained results show a linear response of the PGS electrode in a wide concentrations range. For the lower concentration range, 0.18–7.04 µg L−1, the sensitivity is 11.7 µAcm−2/mgL−1, while for the higher concentration range, 10.6–70.4 µg L−1, the sensitivity is 134 µAcm−2/mgL−1, preserving the linearity of 0.998 and 0.999. The second objective was to determine the effect of the addition of five different types of “green” biowaste on plant growth, VitC content, and antioxidant activity in arugula (Eruca sativa L.) using the developed method. After three weeks of cultivation, small differences in growth and large differences in certain nutritional characteristics were observed. The addition of black coffee makes the soil slightly alkaline and causes a significant increase in VitC content and antioxidant activity.
Visualization of Antimicrobial-Induced Bacterial Membrane Disruption with a Bicolor AIEgen
Authors: Zhou Chengcheng , Ding Zeyu , Guo Qiaoni , Jiang Meijuan
Published: July 2022

Gram-negative bacteria are difficult to kill due to their complex cell envelope, including the outer membrane (OM) and cytoplasmic membrane (CM). To monitor the membranolytic action of antimicrobials on Gram-negative bacteria would facilitate the development of effective antimicrobials. In this paper, an aggregation-induced emission luminogen (AIEgen) with microenvironment-sensitive properties was employed to indicate the interaction of antimicrobials with the OM and CM of Gram-negative bacteria. The damaged extent of OM and CM caused by antimicrobials with the change of dosage and incubation time can be visually captured based on the variation of two emission colors of IQ-Cm responding to OM-defective (green) and CM-disruptive bacteria (orange). Meanwhile, the activity assessment of antimicrobials can be easily realized within 1~2 h based on the distinct response of IQ-Cm to live and dead E. coli, which is much faster than the agar plate culture. This probe may shed light on the understanding of the interaction between the membrane-active antimicrobials and cell envelope of Gram-negative bacteria and contribute to the future development of antimicrobials.
Effect of the Microstructure of ZnO Thin Films Prepared by PLD on Their Performance as Toxic Gas Sensors
Authors: Fasquelle Didier , Députier Stéphanie , Bouquet Valérie , Guilloux-Viry Maryline
Published: July 2022

In 2008, the modified European Restriction of Hazardous Substances (RoHS) directive prohibited the use of hazardous substances such as lead, cadmium, and mercury. As such, an urgent need for lead-free components emerged in Europe. In this frame, we have decided to study the microstructure influence of zinc oxide thin films on the detection of hydrogen sulfide (H2S). Zinc oxide thin films were deposited by PLD on silicon substrates under different conditions to modify the microstructure. In order to compare our demonstrators to current commercial semiconductor gas sensors, measurements under H2S were also performed with sensors from Figaro and Winsen corporations. Gas sensors were therefore implemented by using commercial cases in view to test them with Simtronics gas detector DG477. The good sensitivity values measured at T = 400 °C under 100 ppm H2S, and response times as low as 30 s, definitely confirm that ZnO thin films could be developed for commercial sensors.
Hydrological and Meteorological Variability in the Volga River Basin under Global Warming by 1.5 and 2 Degrees
Authors: Kalugin Andrey
Published: July 2022

The idea of the research to assess the impact of 1.5 °C and 2 °C global warming in the 21st century on the runoff formation in the Volga basin corresponds to the Paris agreement on climate change 2016 with the main goal to keep the global air temperature rise to below 2 °C relative to the pre-industrial level and to take measures to limit warming to 1.5 °C by the end of the 21st century. The purpose of this study was to obtain physically based results of changes in the water regime of the Volga basin rivers under global warming by 1.5 °C and 2 °C relative to pre-industrial values. The physical and mathematical model of runoff generation ECOMAG (ECOlogical Model for Applied Geophysics) was applied in calculations using data from global climate models (GCMs). The estimation of flow anomalies of the Volga River and its major tributaries showed a decrease in annual runoff by 10–11% relative to the period from 1970 to 1999. The largest relative decrease in runoff by 17–20% was noted for the Oka and Upper Volga rivers, while the Kama River had only a 1–5% decrease. The Volga winter runoff increased by 17% and 28% under global warming by 1.5 °C and 2 °C, respectively, and negative runoff anomalies during the spring flood and the summer–autumn period turned out to be in the range of 21 to 23%. Despite the increase in precipitation, the role of evaporation in the water balance of the Volga basin will only increase.
Climate-Induced Fire Hazard in Forests in the Volga Federal District of European Russia during 1992–2020
Authors: Perevedentsev Yuri , Sherstyukov Boris , Gusarov Artyom , Aukhadeev Timur , Mirsaeva Nadezhda
Published: July 2022

This paper shows the relevance of the problem of fire hazard in the forests of the Volga Federal District (VFD) of European Russia. The Nesterov index and the Selyaninov hydrothermal coefficient (HTC) are considered as indicators of fire hazard. The changes in climatic conditions in the VFD during 1955–2018 are shown; a trend towards warming and an increase in aridity in the study region were revealed. The repeatability of various fire hazard classes from May to September was calculated using the Nesterov method. It is shown that in July, the most dangerous situation was in the south of the VFD, where the repeatability of class IV fire hazard reached 27%. Using the HTC index, the degree of aridity of the district in the summer period was estimated. The frequency of the most arid conditions (HTC < 0.5) increases from the north to the south of the district, from 6% (Kirov Region) to 47% (Orenburg Region). Using the TT index, the potential thunderstorm danger in the VFD was assessed. With the help of the constructed maps, the hotspots of the most probable occurrence of thunderstorms were detected. The use of Rosstat data on the number of forest fires from 1992 to 2020 made it possible to consider the spatiotemporal distribution of forest fires in 14 administrative regions of the VFD. The distribution of the number of fires by the regions is shown depending on their forest cover and season. The peak of the number of fires was revealed in 2010, when the entire territory of the study region was covered by a severe drought, as a result of which the area of forests covered by fire increased many times over. In recent years (since 2017), there has been an increase in the area of burned forest due to the active phase of climate warming.
Association between Empagliflozin Use and Electrocardiographic Changes
Authors: Antwi-Amoabeng Daniel , Sathappan Sunil , Beutler Bryce D. , Ulanja Mark B. , Awad Munadel , Gullapalli Nageshwara , Duncan Phillip , Gbadebo T. David
Published: July 2022

Empagliflozin, a sodium-glucose transporter 2 inhibitor, has been shown to bind to late sodium channels in mice cardiomyocytes. We sought to investigate the electrocardiographic (ECG) features associated with empagliflozin use in patients with diabetes mellitus. We compared ECG features of 101 patients before and after initiation of empagliflozin and found that empagliflozin was associated with a significant increase in QRS duration among diabetes patients with heart failure.
Vapor Barrier Properties of Cold Plasma Treated Corn Starch Films
Authors: da Fonseca de Albuquerque Marta , Bastos Daniele , Ţălu Ştefan , Matos Robert , Pires Marcelo , Salerno Marco , da Fonseca Filho Henrique , Simão Renata
Published: July 2022

The development and efficient production of effective bioplastics is a hot topic, required to face up to the issue of the difficult disposal of plastics derived from oil. Among the different natural sources of bioplastics, starch is one of the most promising. However, for most applications, the proper mastering of the surface properties of bioplastic is necessary. We report about the surface modification of extruded corn starch films by means of cold plasma based on helium (He) and hexamethyldisiloxane (HMDSO). The differently treated surfaces were functionally characterized in wettability and water absorption. The nanoscale morphology was assessed by scanning electron microscopy and atomic force microscopy. The obtained images were analyzed by advanced figures describing both texture and amplitude parameters, including fractal behavior. The combined treatment (He/HMDSO) resulted in more homogeneous films with smaller, better-distributed grains compared to the case wherein He was not used. Despite the different morphologies observed, starch coated by HMDSO alone and by He/HMDSO presented similar hydrophobic character, with contact angles higher than 110°. Plasma treatment with HMDSO and He/HMDSO resulted in a significant reduction of absorbed water content without reduction of water vapor permeability. The nanotexture of the films did not present statistically significant differences, in terms of spatial complexities, dominant spatial frequencies, homogeneous void distribution, and surface percolation.
Friction Properties of the Heat-Treated Electroless Ni Coatings Embedded with c-BN Nanoparticles
Authors: Kandeva Mara , Zagorski Mihail , Nikolić Ružica , Stojanović Blaža , But Adrian , Botko František , Piteľ Ján , Vencl Aleksandar
Published: July 2022

The nickel (Ni) coatings without and with embedded (5–7 vol. %) cubic boron nitride (c-BN) nanoparticles (10 nm in diameter) were deposited on carbon steel substrate by an electroless plating process. Coatings were tested in as-deposited and heat-treated (heating at 300 °C for 6 h) conditions. Coating structure characterisation was performed, as well as hardness and roughness measurements. Friction properties were tested in dry and in water (seawater) lubricated contact conditions, with bronze as a counter-body material. Both static and kinetic coefficients of friction were measured for two different surface texture preparations (initial and working). The first surface texture simulated the running-in condition, and the second surface texture represented the steady-state conditions. The enhancement of the abrasive and erosive wear resistance of heat-treated electroless Ni coatings with embedded c-BN nanoparticles was already proved in our previous studies. This study aims to investigate those influences on friction properties of electroless Ni coatings in different sliding conditions. The results show that the coefficients of friction did not differ too much between the coatings and that the surface roughness and presence of seawater had a much stronger influence.
Preparation and Characterization of the Cr-Nanodiamonds/MoN Coatings with Performant Mechanical Properties
Authors: Chayeuski Vadzim , Taleb Abdelhafed , Zhylinski Valery , Kuleshov Andrei , Shtempliuk Roman
Published: July 2022

This paper presents the results of a study on the preparation and characterization of a Cr-DND/MoN detonation chromium-nanodiamond coating deposited on cemented tungsten carbide (WC–3 wt.% Co) mill blades using Arc-PVD and electrodeposition methods. The physical and mechanical characteristics of the coatings were investigated by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), XRD analysis, Raman spectroscopy, micro-identification, and scratch test (evaluation of the coating adhesion). It was shown that the Cr-DND/MoN coating consists of successive layers of Cr-DND (top), Cu (middle) and MoN (bottom) with separate phases of γ-Mo2N, α-Mo, α-Cu, Cr-DND and nanodiamonds. The Cr-DND composite electrochemical coating (CEC) was deposited from the conventional chromium plating electrolyte with the addition of nanodiamonds. The copper interlayer was deposited by the Arc-PVD method on the surface of the MoN coating to improve the adhesion strength of the Cr-DND CEC. The coating showed an optimum microhardness of about 14 ± 1 GPa and good adhesion with a critical load Lc of about 93 N. In addition to the expected experimental results, the coating has high wear resistance, confirmed by scratch tests.
The Correlation of Plasma Characteristics to the Deposition Rate of Plasma Polymerized Methyl Methacrylate Thin Films in an Inductively Coupled Plasma System
Authors: Hsieh Stephen T. , Mishra Himanshu , Bolouki Nima , Wu Weite , Li Chuan , Hsieh Jang-Hsing
Published: July 2022

A plasma system attached with one internal coil (for generating inductively coupled plasma) and two sputtering carbon targets was set up to deposit PP-MMA (plasma polymerized methyl methacrylate) thin films. PP-MMA was used as a model material in the present study. In the experiment, the working pressure and Ar/MMA flow ratio were varied, which resulted in the change in plasma conditions as well as the deposition rates. The optical emission spectroscopy (OES) method was applied to identify the presence of the excited species related to the fragmented monomer. In addition, the electron temperature and electron density were determined using the modified Boltzmann plot and line-ratio method, according to the measured OES spectra. The deposition rate of the PMMA film was then correlated with the determined plasma characteristics. To determine the vibrational modes of the deposited PP-MMA films, Fourier transformed infrared spectrometry (FTIR) was used. The highest deposition rate of PP-MMA could be obtained with the optimized working pressure and Ar/MMA volume ratio. This could be related to the plasma characteristics that contribute to the fragmentation of the monomer in the plasma.
Fully Relaxed, Crack-Free AlGaN with upto 50% Al Composition Grown on Porous GaN Pseudo-Substrate
Authors: Hatui Nirupam , Collins Henry , Kayede Emmanuel , Pasayat Shubhra S. , Li Weiyi , Keller Stacia , Mishra Umesh K.
Published: July 2022

Fully relaxed, crack free, smooth AlxGa1−xN layers with up to 50% Al composition were demonstrated on pseudo-substrates composed of dense arrays of 10 × 10 µm2 compliant porous GaN-on-porous-GaN tiles. The AlGaN layers were grown in steps for a total of 1.3 µm. The growth conditions necessary to demonstrate high quality films at higher Al compositions also suppressed any sidewall growth.
Performance Analysis of DAST Material-Assisted Photonic-Crystal-Based Electrical Tunable Optical Filter
Authors: Goyal Amit Kumar , Kumar Ajay , Massoud Yehia
Published: July 2022

In this paper, a 4-N,N-dimethylamino-4′-N′-methyl-stilbazolium tosylate (DAST) material assisted one-dimensional photonic-crystal-based (1D-PhC) tunable optical filter is presented. The design comprises a bilayer 1D-PhC structure having DAST as an electro-optic material. The device parameters are configured to filter out the 632.8 nm wavelength from the reflection spectrum. The analysis shows that by illuminating the device with poly-chromatic light at an incident angle of 45.07°, the filtered wavelength exhibits transmission maxima having FWHM of less than 1nm. The analytical results also demonstrate the post fabrication 60 nm electrical tuning of the filtered wavelength by using only ±5 V applied potential. The structure also exhibits a very stable filter response up to 40% variations in optical thickness. Thus, the proposed design possesses the advantage in terms of low voltage wavelength tuning, stable response, easy fabrication and integration capability in integrated circuits.
Management of Myelofibrosis during Treatment with Ruxolitinib: A Real-World Perspective in Case of Resistance and/or Intolerance
Authors: Breccia Massimo , Palandri Francesca , Guglielmelli Paola , Palumbo Giuseppe Alberto , Malato Alessandra , Mendicino Francesco , Ricco Alessandra , Sant’Antonio Emanuela , Tiribelli Mario , Iurlo Alessandra
Published: July 2022

The development and approval of ruxolitinib, the first JAK1/2 inhibitor indicated to treat myelofibrosis, has improved patient outcomes, with higher spleen and symptoms responses, improved quality of life, and overall survival. Despite this, several unmet needs remain, including the absence of resistance criteria, suboptimal response, the timing of allogeneic transplant, and the management of patients in case of intolerance. Here, we report the results of the second survey led by the “MPN Lab” collaboration, which aimed to report physicians’ perspectives on these topics. As in our first survey, physicians were selected throughout Italy, and we included those with extensive experience in treating myeloproliferative neoplasms and those with less experience representing clinical practice in the real world. The results presented here, summarized using descriptive analyses, highlight the need for a clear definition of response to ruxolitinib as well as recommendations to guide the management of ruxolitinib under specific conditions including anemia, thrombocytopenia, infections, and non-melanoma skin cancers.
Increased MIB-1 Labeling Index Is Associated with Abducens Nerve Morbidity in Primary Sporadic Petroclival Meningioma Surgery: Beyond Location and Approach
Authors: Wach Johannes , Lampmann Tim , Güresir Ági , Vatter Hartmut , Herrlinger Ulrich , Becker Albert , Toma Marieta , Hölzel Michael , Güresir Erdem
Published: July 2022

Abducens nerve palsy is a severe dysfunction after petroclival meningioma (PC MNG) surgery. The objective of this investigation was to analyze abducens nerve outcomes in patients who underwent the retrosigmoid approach in relation to the MIB-1 index. Thirty-two patients with primary sporadic PC MNG were retrospectively analyzed. Mean follow-up was 28.0 months. Analysis of the MIB-1 index was performed to evaluate the abducens nerve outcome. An optimal MIB-1 index cut-off value (<4/≥4) in the association with postoperative CN VI palsy was determined by ROC analysis (AUC: 0.74, 95% CI: 0.57–0.92). A new-onset CN VI palsy was present in 7 cases (21.88%) and was significantly associated with an increased MIB-1 index (≥4%, p = 0.025) and a peritumoral edema in the brachium pontis (p = 0.047) which might be caused by the increased growth rate. Tumor volume, cavernous sinus infiltration, auditory canal invasion, and Simpson grading were not associated with new CN VI deficits. Six (85.7%) of the 7 patients with both an increased MIB-1 index (≥4%) and new abducens nerve palsy still had a CN VI deficit at the 12-month follow-up. A peritumoral edema caused by a highly proliferative PC MNG with an elevated MIB-1 index (≥4%) is associated with postoperative abducens nerve deficits.
Bortezomib, Lenalidomide and Dexamethasone Combination Induced Complete Remission in Relapsed/Refractory Plasmablastic Lymphoma: Case Report of a Potential Novel Treatment Approach
Authors: Sabry Waleed , Wu Yue , Kodad Shruthi Ganeshappa
Published: July 2022

Plasmablastic lymphoma is a rare subtype of large B-cell lymphoma characterised by an aggressive clinical course with frequent relapses and refractoriness to chemotherapy. It is usually associated with HIV, however, it can also be seen in immunocompetent patients. It has distinct pathological characteristics, such as plasmablastic morphology and lack of CD20 expression. These characteristics pose a clinical and pathological challenge. There is no standard of care established in this entity. In this case report, we described a novel bortezomib-based plasma cell targeted regimen in a HIV-negative patient refractory to chemotherapy.
Investigation of the Genetic Diversity of Dagestan Mountain Cattle Using STR-Markers
Authors: Volkova Valeria V. , Abdelmanova Alexandra S. , Deniskova Tatiana E. , Romanenkova Olga S. , Khozhokov Abdusalam A. , Ozdemirov Alimsoltan A. , Sermyagin Alexander A. , Zinovieva Natalia A.
Published: July 2022

The Dagestan Mountain (DM) cattle breed was established to provide cheese and milk products to mountain dwellers in the specific conditions of the Republic of Dagestan in Southern Russia. Only 650 head of DM cattle were registered in 2020, and their “breed purity” is questionable. We aimed to assess the genetic diversity and population structure of modern DM cattle using short tandem repeat (STR) markers. The sample included 150 animals collected from private owners in Dagestan during a scientific expedition (n = 32) and provided by a gene pool farm (n = 118). An additional 166 samples from other cattle breeds distributed in the breeding zone of DM cattle were used as the comparison groups. The genotypes for the 11 STR loci recommended by ISAG were obtained using a genetic analyzer. We identified a high level of genetic diversity as revealed by allelic richness calculations (Ar = 6.82 vs. 4.38–5.82 in other cattle breeds) and observed heterozygosity indices (uHe = 0.76 vs. 0.65–0.72). Based on the STRUCTURE results, animals with low levels of admixture with other breeds were found within the DM cattle, which can be considered as candidates for use in germplasm conservation programs.
Prioritizing Plants around the Cross-Border Area of Greece and the Republic of North Macedonia: Integrated Conservation Actions and Sustainable Exploitation Potential
Authors: Krigas Nikos , Karapatzak Eleftherios , Panagiotidou Marina , Sarropoulou Virginia , Samartza Ioulietta , Karydas Antonis , Damianidis Christos K. , Najdovski Boris , Teofilovski Aco , Mandzukovski Dejan , Stipanović Viktorija Brndevska , Papanastasi Katerina , Kapagianni Pantelitsa D. , Fotakis Dimitrios , Grigoriadou Katerina , Tsoktouridis Georgios , Andonovski Vlatko , Maloupa Eleni
Published: July 2022

Plants know no political borders and some of them are restricted to small geographical territories of different countries in which they are endemic. In this study, we prioritized plants (PPs) of the cross-border area of Greece and the Republic of North Macedonia that are already threatened or nearly so (PPA), those which occur exclusively on either side of borderline and/or nearby countries (PPB), or those which are uncommon and rare in this region (PPC) with the aim to document in-situ the species-specific risks-threats; offer ex-situ conservation for them as a back-up solution for future re-introductions and sustainable exploitation; and raise public awareness and alertness about the importance of local biodiversity. In the framework of the project Conse-pp, 20 botanical expeditions were performed in 75 selected areas to collect samples and suitable propagation material from 130 PPs (147 accession numbers), also recording all types of threats-identified in-situ for each of them. No ex-situ conservation was detected for 40 PPs worldwide while for another 12 PPs only limited attempts have been made to date. The fully documented PPs are currently maintained under ex- situ conservation and acclimatization in the Balkan Botanic Garden of Kroussia (BBGK). In total, 156 propagation trials (sexual or asexual) have been made for these PPs to develop species-specific propagation protocols. Consequently, the production of new plant stocks raised ex-situ was achieved (n = 3254 individuals; first-time ex-situ conservation for 40 taxa), and this has enabled the establishment of three awareness-raising sites with PPs: (i) 1000 plants of 70 PPs in the newly designed Kardia Botanical Park in Thermi (Greece); (ii) 850 plants of 104 PPs in the phytodiversity awareness spot in BBGK and 130 individual PPs in the Garden of Environmental Awareness; and (iii) 42 plants of 14 PP taxa delivered to the botanic garden of Ss. Cyril and Methodius University in Skopje. This project outlines the necessity of the development of common plant conservation strategies for threatened plants in cross-border areas of neighboring countries and presents an integrated approach allowing for sustainable development and future in-situ protection measures and actions in the studied cross-border area.
Research Advances in Toona sinensis, a Traditional Chinese Medicinal Plant and Popular Vegetable in China
Authors: Zhao Qian , Zhong Xiu-Lai , Zhu Shun-Hua , Wang Kun , Tan Guo-Fei , Meng Ping-Hong , Zhang Jian
Published: July 2022

Toona sinensis, a perennial and deciduous tree belonging to the Meliaceae family, has been cultivated for more than 2000 years in China. Storing the buds of T. sinensis is difficult, as it is easy for them to rot during storage, which seriously affects their edible and commodity value. Young leaves and buds of T. sinensis plants are excellent source of flavonoids, terpenoids, phenylpropanoids, and more. In addition, the bioactive components of T. sinensis possess numerous health benefits, such as antiviral, antioxidant, anti-cancer, anti-inflammatory, and hypoglycemic effects. In this review, we summarize the storage and preservation, nutritional components, specific chemical compounds, pharmacological value, function genes, and omics of T. sinensis. This review aims to provide basic knowledge for subsequent future research seeking to understand the comprehensive biology and use of T. sinensis as a favored Chinese food and pharmacological resource.
Introduction to the Special Issue “Aquatic Insects: Biodiversity, Ecology, and Conservation Challenges”
Authors: Vilenica Marina , Vuataz Laurent , Yanai Zohar
Published: July 2022

In non-marine environments, insects comprise one of the most species-rich and abundant groups of organisms [...]
Diversity of Nearctic Dragonflies and Damselflies (Odonata)
Authors: Abbott John C. , Bota-Sierra Cornelio A. , Guralnick Robert , Kalkman Vincent , González-Soriano Enrique , Novelo-Gutiérrez Rodolfo , Bybee Seth , Ware Jessica , Belitz Michael W.
Published: July 2022

Rarely have studies assessed Odonata diversity for the entire Nearctic realm by including Canada, the United States, and Mexico. For the first time, we explored Odonata diversity in this region according to a definition of natural community assemblages and generated species distribution models (SDMs). Species occurrence data were assembled by reviewing databases of specimens held by significant Odonata repositories and through an extensive search of literature references. Species were categorized as forest-dependent or non-forest-dependent, as lentic or lotic-dependent, and according to conservation status. Predicted distributions were stacked for all species across their entire ranges, including areas outside of the Nearctic. Species richness and corrected weighted endemism (CWE) were then calculated for each grid cell. We found a pattern of greater species richness in the eastern portion of the Nearctic, which can be explained by the higher aquatic habitat diversity at micro and macroscales east of the Rocky Mountains, promoting niche partitioning and specialization. In the Nearctic region, the southeastern US has the highest number of endemic species of dragonflies and damselflies; this degree of endemism is likely due to glacial refuges providing a foundation for the evolution of a rich and unique biota.
The Biodiversity of Calcaxonian Octocorals from the Irish Continental Slope Inferred from Multilocus Mitochondrial Barcoding
Authors: Morrissey Declan , Untiedt Candice B. , Croke Karen , Robinson Aisling , Turley Eva , Allcock A. Louise
Published: July 2022

Deep-sea corals are important benthic inhabitants that support the biodiversity and function of the wider faunal community; however, their taxonomy is underdeveloped and their accurate identification is often difficult. In our study, we investigated the utility of a superextended (>3000 bp) barcode and explored the effectiveness of various molecular species delimitation techniques with an aim to put upper and lower bounds on the estimated number of calcaxonian species in Irish waters. We collected 112 calcaxonians (70 Keratoisididae, 22 Primnoidae, 20 Chrysogorgiidae) and one chelidonisid from the Irish continental slope and sequenced a 3390 bp DNA barcode comprising four mitochondrial regions (mtMutS, COI + igr1, 16S rRNA-ND2, and igr4), recovering 38 haplotypes. Individuals that shared a haplotype were often morphologically distinct, and we thus undertook detailed morphological work, including SEM of sclerites, on one representative of each morphotype within each haplotype. GMYC, bGMYC, and mPTP returned incongruent estimates of species numbers. In total, there are between 25 and 40 species, although no definitive number could be assigned, primarily due to poorly defined keratoisidid species boundaries. As expected, the superextended barcode provided greater discrimination power than single markers; bGMYC appeared to be the most effective delimiter. Among the identified species were Chelidonisis aurantiaca, collected deeper than previously known at 1507 m, and Calyptrophora clinata, recorded for the second time from the Northeast Atlantic. A full understanding of the diversity and distribution of calcaxonians requires substantial taxonomic work, but we highlight the Irish continental slope as harbouring significant diversity.
Oropharyngeal Candidiasis among Egyptian COVID-19 Patients: Clinical Characteristics, Species Identification, and Antifungal Susceptibility, with Disease Severity and Fungal Coinfection Prediction Models
Authors: Khalil Mahmoud A. F. , El-Ansary Mahmoud R. M. , Bassyouni Rasha H. , Mahmoud Eman E. , Ali Inas A. , Ahmed Tarek I. , Hassan Essam A. , Samir Tamer M.
Published: July 2022

The study aimed to investigate the causative species, antifungal susceptibility, and factors associated with oropharyngeal candidiasis (OPC) among Egyptian COVID-19 patients. This is an observational, case-controlled, single-center study that included three groups: COVID-19 patients (30), COVID-19 patients with OPC (39), and healthy individuals (31). Patients’ demographic data (age, sex), laboratory tests, comorbidities, treatment, and outcomes were included. Candida species were isolated from COVID-OPC patient’s oropharyngeal swabs by convenient microbiological methods. Isolated strains were tested for antimicrobial susceptibility, biofilm production, aspartyl protease, and phospholipase activities. The most common respiratory symptoms reported were dyspnea (36/39; 92.4%) and cough (33/39; 84.7%). Candida albicans was the most common isolated species, accounting for 74.36% (29/39), followed by Candida tropicalis and Candida glabrata (15.38% and 10.26%, respectively). Amphotericin was effective against all isolates, while fluconazole was effective against 61.5%. A total of 53.8% of the isolates were biofilm producers. The phospholipase activity of C. albicans was detected among 58.6% (17/29) of the isolates. Significant variables from this study were used to create two equations from a regression model that can predict the severity of disease course and liability to fungal infection, with a stativity of 87% and 91%, respectively. According to our findings, COVID-19 patients with moderate to severe infection under prolonged use of broad-spectrum antibiotics and corticosteroids should be considered a high-risk group for developing OPC, and prophylactic measures are recommended to be included in the treatment protocols. In addition, due to the increased rate of fluconazole resistance, other new antifungals should be considered.
New Sagittal and Vertical Cephalometric Analysis Methods: A Systematic Review
Authors: Kotuła Jacek , Kuc Anna Ewa , Lis Joanna , Kawala Beata , Sarul Michał
Published: July 2022

Cephalometric analysis is an essential tool used in orthodontic diagnosis and treatment planning. The main objectives of correct cephalometric analysis include resolving anteroposterior and vertical maxillary and mandibular base discrepancies. For a diagnostic tool to be of value, it should be precise, reliable and reproducible. Unfortunately, according to some studies, the accuracy of input and, therefore, the diagnostic reliability of some of the points and measurements may not be satisfactory. To this end, new cephalometric measurements are being developed with increased precision. In order to properly and definitively determine the usefulness of a given measurement in cephalometric diagnosis, it is necessary to carry out a critical evaluation of available studies. The aim of this systematic review was to evaluate the available scientific literature describing new landmarks and reference linear and angular measurements of 2D cephalometric analyses assessing the sagittal and vertical discrepancy in the position of jaw bases since the last systematic review in 2013. The secondary aim was to assess the accuracy and reliability of new anthropometric landmarks and reference planes in relation to those used previously, and their instability in relation to growth and orthodontic tooth movements. To carry out the intended plan, electronic databases such as PubMed, Scholar Google, Web of Science and Pro Quest were searched using specific keywords. Initially, a total of 1451 articles were retrieved. Then, duplicate articles in all databases were excluded from the resulting publications. The results showed that despite such a high number of articles published in peer-reviewed scientific journals, only 12 studies on new cephalometric analyses in the sagittal plane and 4 studies on new cephalometric analyses in the horizontal plane met the criteria and, as a result, were included in the review.
The Diagnostic Differentiation Challenge in Acute Appendicitis: How to Distinguish between Uncomplicated and Complicated Appendicitis in Adults
Authors: Skjold-Ødegaard Benedicte , Søreide Kjetil
Published: July 2022

(1) Background: How to best define, diagnose and differentiate uncomplicated from complicated acute appendicitis remains debated. Hence, the aim of this review was to present an overview of the current knowledge and emerging field of acute appendicitis with a focus on the diagnostic differentiation of severity currently subject to ongoing investigations. (2) Methods: We conducted a PubMed search using the MeSH terms “appendicitis AND severity” and “appendicitis AND classification”, with a focus on studies calling appendicitis as ‘uncomplicated’ or ‘complicated’. An emphasis on the last 5 years was stressed, with further studies selected for their contribution to the theme. Further studies were retrieved from identified full-text articles and included per the authors’ discretion. (3) Results: The assumption that appendicitis invariably will proceed to perforation has been outdated. Both uncomplicated and complicated appendicitis exist with likely different pathophysiology. Hence, this makes it important to differentiate disease severity. Clinicians must diagnose appendicitis, but, in the next step, also differentiate between uncomplicated and complicated appendicitis in order to allow for management decisions. Diagnostic accuracy without supportive imaging is around 75–80% and, based on clinical judgement and blood tests alone, the negative appendectomy rate has been described as high as 36%. More research is needed on available biomarkers, and the routine use of imaging still remains debated. Scoring systems have the potential to improve diagnostic accuracy, but no scoring system has yet been validated for differentiating disease severity. Currently, no universally agreed definition exists on what constitutes a complicated appendicitis. (4) Conclusions: Uncomplicated and complicated appendicitis appear to have different pathophysiology and should be treated differently. The differentiation between uncomplicated and complicated appendicitis remains a diagnostic challenge.