bluuebunny/crossref_metadata_2025 huggingface dataset:
Management and outcomes of spontaneous coronary artery dissection: a systematic review of the literature
Authors: Petrović Milovan , Miljković Tatjana , Ilić Aleksandra , Kovačević Mila , Čanković Milenko , Dabović Dragana , Stojšić Milosavljević Anastazija , Čemerlić Maksimović Snežana , Jaraković Milana , Andrić Dragica , Golubović Miodrag , Bjelobrk Marija , Bjelić Snežana , Tadić Snežana , Slankamenac Jelena , Apostolović Svetlana , Djurović Vladimir , Milovančev Aleksandra
Published: January 2024

Background

Contemporary management of spontaneous coronary artery dissection (SCAD) is still controversial. This systematic review of the literature aims to explore outcomes in the patients treated with conservative management vs. invasive strategy.

Methods

The PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines were followed when we extensively searched three electronic databases: PubMed, ScienceDirect, and Web of Science, for studies that compared conservative vs. invasive revascularization treatment outcomes for patients with SCAD from 2003 to 2023. The outcomes of interest were all-cause death and major adverse cardiovascular events (MACE), including acute coronary syndrome (ACS), heart failure (HF), need for additional revascularization, target vessel revascularization (TVR), SCAD recurrence, and stroke.

Results

The systematic review included 13 observational studies evaluating 1,801 patients with SCAD. The overall mean age was 49.12 +/− 3.41, and 88% were females. The overall prevalence of arterial hypertension was 33.2%, hyperlipidemia, 26.9%, smoking, 17.8%, and diabetes, 3.9%. Approximately 48.5% of the patients were diagnosed with non-ST elevated myocardial infarction (NSTEMI), 36.8% with ST elevated myocardial infarction (STEMI), 3.41% with unstable angina, 0.56% with stable angina, and 0.11% were diagnosed with various types of arrhythmias. The left anterior descending artery (LAD) was the most common culprit lesion in 51% of the patients. There were initially 65.2% of conservatively treated patients vs. 33.4% that underwent percutaneous coronary intervention (PCI) or 1.28% that underwent coronary artery bypass graft (CABG). SCAD-PCI revascularization was associated with a variable range of PCI failure. The most common complications were hematoma extension and iatrogenic dissection. SCAD-PCI revascularization frequently required three or more stents and had residual areas of dissection. The overall reported in-hospital and follow-up mortality rates were 1.2% and 1.3%, respectively. The follow-up range across studies was 7.3–75.6 months. The authors reported variable prevalence of MACE, recurrent SCAD up to 31%, ACS up to 27.4%, TVR up to 30%, repeat revascularization up to 14.7%, UA up to 13.3%, HF up to 17.4%, and stroke up to 3%.

Conclusion

Our results highlight that conservative treatment should be the preferred method of treatment in patients with SCAD. PCI revascularization is associated with a high prevalence of periprocedural complications. SCAD poses a considerable risk of MACE, mainly associated with TVR, ACS, and recurrent SCAD.
3D seismic facies recognition based on region growing
Authors: Wang Youtao , Ma Xiong , Gui Zhixian , Chen Xueguo , Wang Yuelei
Published: January 2024

Seismic facies analysis is important for oil and gas exploration. The conventional seismic facies recognition methods are implemented manually with high workload and low accuracy. Therefore, how to obtain seismic facies characteristics quickly, efficiently, and accurately is an urgent requirement in seismic facies research. To alleviate this issue, we propose a novel seismic facies recognition method based on the region growing algorithm with expert knowledge constraint. The processes of this algorithm are as follows: firstly, we select high-density 3D seismic data in the target area for seismic facies identification. Then, we utilize expert knowledge to define the priori geological constraint for regional growing algorithm. Finally, the region growing algorithm is used to pick up and divide different 3D seismic facies boundaries in the study area. The verification of known geological knowledge proves that the results are reasonable and reliable. The accuracy and efficiency of the proposed seismic facies identification method based on region growing are significantly improved.
Stable operating limits and improvement methods for hydropower and photovoltaic integration through MMC-HVDC systems
Authors: Peng Maolan , Feng Lei , Zhang Shuwen , Zhao Wei
Published: January 2024

This paper addresses the critical need to determine the stable operating limit of modular multilevel converter-based high voltage direct current (MMC-HVDC) systems, particularly concerning the integration of extensive renewable energy sources. To achieve this, the steady-state mathematical model and state-space model of bundled hydropower and photovoltaic integration through MMC-HVDC systems are established. A novel methodology considering steady-state and small-signal stability constraints is proposed to compute the stable operating region of the system. The quantitative assessment reveals that diminishing AC system short-circuit capacities amplify restrictions from small-signal stability constraints, thereby reducing the system's stable operating region. Eigenvalue and participation factor analyses shed light on the pivotal factors affecting small-signal stability in weak AC systems. To expand the system's stable operating region, a supplementary frequency damping control strategy is proposed. The theoretical analysis and calculation results are validated by building a simulation model for the bundled hydropower and photovoltaic integration through MMC-HVDC systems in PSCAD/EMTDC.
Confidence relative off-targets distance-based multi-dimensional transparency evaluation of distribution station area
Authors: Ren Jie , Li Lisheng , Li Shuai , Liu Minglin , Fang Mu , Zhang Shidong , Liu Wenbin , Liu Yang , Yu Haidong
Published: January 2024

With the large-scale integration of distributed renewable energy into low-voltage distribution station areas, rapidly growing services put forward new requirements on transparent monitoring. In order to accurately and objectively quantify the transparency of various distribution station areas and measure the impact of different dimensional indexes on the transparency evaluation of distribution station areas, this paper proposes a confidence relative off-target distance-based multi-dimensional transparency evaluation method. First, a multi-dimensional transparency evaluation index system with electrical and communication integration is constructed. Second, an improved gray target model combining both positive and negative target vectors is established to realize bi-directional quantitative analysis. Then, the relative off-target distance is calculated based on the endowment coefficient by leveraging both subjective and objective weights. Finally, the confidence of the relative off-target distance is calculated based on fuzzy entropy to improve the reliability of transparency evaluation. The simulation results show that this method can effectively distinguish the transparency gap between different distribution station areas, identify the single dimensional index with the greatest contribution or potential, and verify the effectiveness of the proposed method applied to the transparency evaluation of distribution station areas.
A smooth grid connection strategy for compressed air energy storage based on adaptive PI control
Authors: Wang Dajiang , Sun Yaxin , Ge Yaming , Li Jie , Yan Chaoyang , Meng Jianhui
Published: January 2024

In the context of the application of compressed air energy storage system participating in power grid regulation, a large capacity of compressed air energy storage accessed to or off from the power grid will bring instability to the system, and there will be voltage and current impact during off-grid operation, which will pose a threat to system security. Therefore, this paper puts forward the control strategy of compressed air energy storage for both grid-connected and off-grid, and proposes a smooth grid-connected strategy of compressed air energy storage based on adaptive PI control, which can better improve the problem of excessive impulse current during the connection of compressed air energy storage. Finally, a simulation model is built in MATLAB/Simulink to verify the effectiveness and superiority of the proposed control strategy.
Case report: A founder UGDH variant associated with developmental epileptic encephalopathy in Saudi Arabia
Authors: Alaamery Manal , Massadeh Salam , Aldarwish Manar , Albesher Nour , Aljawini Nora , Alahmed Othman , Kashgari Amna , Walsh Christopher A. , Eyaid Wafaa
Published: January 2024

Congenital disorders of glycosylation (CDG) are a group of more than 100 rare genetic disorders characterized by impaired glycosylation of proteins and lipids. The clinical presentation of CDG varies tremendously, from single-organ to multi-organ involvement and from prenatal death to a normal adult phenotype. In this case study, we report a large consanguineous family with multiple children suffering from cerebral palsy, seizure, developmental and epileptic encephalopathy, and global developmental delay. Whole-exome sequencing (WES) analysis revealed a homozygous variant in the UDP-glucose dehydrogenase (UGDH) gene (c.950G>A; p.R317Q) which segregates with the familial phenotype with a plausible autosomal recessive mode of inheritance, indicating a potential disease-causing association. The UGDH gene encodes the UDP-glucose dehydrogenase, a key enzyme in the synthesis of specific extracellular matrix constituents (proteoglycans and glycolipids) involved in neural migration and connectivity during early brain development. Many pathogenic mutations of UGDH have been reported in recent literature works. However, the variant identified in this study has been observed only in the Saudi population (13 families) and not in any other ethnic background, suggesting that it may be an ancient founder mutation.
An in silico approach to identify potential downstream targets of miR-153 involved in Alzheimer’s disease
Authors: Amber Sanila , Zahid Saadia
Published: January 2024

Background: In recent years, microRNAs (miRNAs) have emerged as key players in the pathophysiology of multiple diseases including Alzheimer’s disease (AD). Messenger RNA (mRNA) targeting for regulation of gene expression by miRNAs has been implicated in the annotation of disease pathophysiology as well as in the explication of their starring role in contemporary therapeutic interventions. One such miRNA is miR-153 which mediates the survival of cortical neurons and inhibits plaque formation. However, the core mRNA targets of miR-153 have not been fully illustrated.

Objective: The present study aimed to elucidate the potential involvement of miR-153 in AD pathogenesis and to reveal its downstream targets.

Methods: miRanda was used to identify AD-associated targets of miR-153. TargetScan, PicTar, miRmap, and miRDB were further used to validate these targets. STRING 12 was employed to assess the protein-protein interaction network while Gene ontology (GO) analysis was carried out to identify the molecular functions exhibited by these gene targets.

Results:In silico analysis using miRanda predicted five important AD-related targets of miR-153, including APP, SORL1, PICALM, USF1, and PSEN1. All five target genes are negatively regulated by miR-153 and are substantially involved in AD pathogenesis. A protein interaction network using STRING 12 uncovered 30 potential interacting partners for SORL1, PICALM, and USF1. GO analysis revealed that miR-153 target genes play a critical role in neuronal survival, differentiation, exon guidance, amyloid precursor protein processing, and synapse formation.

Conclusion: These findings unravel the potential role of miR-153 in the pathogenesis of AD and provide the basis for forthcoming experimental studies.
Critical mineral demands may limit scaling of green hydrogen production
Authors: Greenwald Julia E. , Zhao Mervin , Wicks Douglas A.
Published: January 2024

Hydrogen (H2) is widely viewed as critical to the decarbonization of industry and transportation. Water electrolysis powered by renewable electricity, commonly referred to as green H2, can be used to generate H2 with low carbon dioxide emissions. Herein, we analyze the critical mineral and energy demands associated with green H2 production under three different hypothetical future demand scenarios, ranging from 100–1,000 Mtpa H2. For each scenario, we calculate the critical mineral demands required to build water electrolyzers (i.e., electrodes and electrolyte) and to build dedicated or additional renewable electricity sources (i.e., wind and solar) to power the electrolyzers. Our analysis shows that scaling electrolyzer and renewable energy technologies that use platinum group metals and rare earth elements will likely face supply constraints. Specifically, larger quantities of lanthanum, yttrium, or iridium will be needed to increase electrolyzer capacity and even more neodymium, silicon, zinc, molybdenum, aluminum, and copper will be needed to build dedicated renewable electricity sources. We find that scaling green H2 production to meet projected net-zero targets will require ∼24,000 TWh of dedicated renewable energy generation, which is roughly the total amount of solar and wind projected to be on the grid in 2050 according to some energy transition models. In summary, critical mineral constraints may hinder the scaling of green H2 to meet global net-zero emissions targets, motivating the need for the research and development of alternative, low-emission methods of generating H2.
Current situation and trend of non-coding RNA in rheumatoid arthritis: a review and bibliometric analysis
Authors: Wei Zehong , Li Huaiyu , Lv Senhao , Yang Junping
Published: January 2024

Background

Rheumatoid arthritis (RA) is a chronic, systemic autoimmune disease that affects multiple joints and has adverse effects on various organs throughout the body, often leading to a poor prognosis. Recent studies have shown significant progress in the research of non-coding RNAs (ncRNAs) in RA. Therefore, this study aims to comprehensively assess the current status and research trends of ncRNAs in RA through a bibliometric analysis.

Methods

This study retrieved articles relevant to ncRNAs and RA from the Science Citation Index Expanded Database of the Web of Science Core Collection between January 1st, 2003, and July 31st, 2023. The relevant articles were screened based on the inclusion criteria. VOSviewer and CiteSpace are utilized for bibliometric and visual analysis.

Results

A total of 1697 publications were included in this study, and there was a noticeable increase in annual publications from January 1st, 2003, to July 31st, 2023. China, the United States, and the United Kingdom were the most productive countries in this field, contributing to 43.81%, 13.09%, and 3.87% of the publications. Anhui Medical University and Lu Qianjin were identified as the most influential institution and author. Frontiers In Immunology stood out as the most prolific journal, while Arthritis & Rheumatology was the most co-cited journal. Additionally, the research related to “circular RNA”, “oxidative stress”, “proliferation”, and “migration” have emerged as new hotspots in the field.

Conclusion

In this study, we have summarized the publication characteristics related to ncRNA and RA and identified the most productive countries, institutions, authors, journals, hot topics, and trends.
The effect of monitoring complexity on stakeholder acceptance of CO2 geological storage projects in the US gulf coast region
Authors: Atkinson Lucy , Dankel Dorothy J. , Romanak Katherine D.
Published: January 2024

Environmental monitoring at geologic CO2 storage sites is required by regulations for the purposes of environmental protection and emissions accounting in the case of leakage to surface. However, another very important goal of environmental monitoring is to assure stakeholders that the project is monitored for safety and effectiveness. With current efforts to optimize monitoring for cost-effectiveness, the question remains: will optimization of monitoring approaches degrade stakeholder assurance, or do heavily-instrumented sites communicate higher risk to a stakeholder? We report the results of a stakeholder survey in Gulf Coast states of the US where carbon capture and storage (CCS) is developing quickly. We rely on a 2 by 2 factorial experiment in which we manipulate message complexity (complex v. simple) and social norm (support from scientists v. support from community members). Subjects were randomly assigned to one of four conditions: 1) complex message with scientist support; 2) complex message with community member support; 3) simple message with scientist support; or 4) simple message with community member support. In addition to the experimental stimuli, subjects were also asked about their need for cognition, attitudes toward science and scientists, attitudes about climate change and support for carbon capture and storage (CCS). Our sample is drawn from residents in states bordering the western Gulf of Mexico (Texas, Louisiana, Florida) where CO2 geologic storage is being planned both onshore and offshore. The results offer important implications for public outreach efforts to key stakeholders.
Ophthalmic implications of biological threat agents according to the chemical, biological, radiological, nuclear, and explosives framework
Authors: Curran Emma H. , Devine Max D. , Hartley Caleb D. , Huang Ye , Conrady Christopher D. , Debiec Matthew R. , Justin Grant A. , Thomas Joanne , Yeh Steven
Published: January 2024

As technology continues to evolve, the possibility for a wide range of dangers to people, organizations, and countries escalate globally. The United States federal government classifies types of threats with the capability of inflicting mass casualties and societal disruption as Chemical, Biological, Radiological, Nuclear, and Energetics/Explosives (CBRNE). Such incidents encompass accidental and intentional events ranging from weapons of mass destruction and bioterrorism to fires or spills involving hazardous or radiologic material. All of these have the capacity to inflict death or severe physical, neurological, and/or sensorial disabilities if injuries are not diagnosed and treated in a timely manner. Ophthalmic injury can provide important insight into understanding and treating patients impacted by CBRNE agents; however, improper ophthalmic management can result in suboptimal patient outcomes. This review specifically addresses the biological agents the Center for Disease Control and Prevention (CDC) deems to have the greatest capacity for bioterrorism. CBRNE biological agents, encompassing pathogens and organic toxins, are further subdivided into categories A, B, and C according to their national security threat level. In our compendium of these biological agents, we address their respective CDC category, systemic and ophthalmic manifestations, route of transmission and personal protective equipment considerations as well as pertinent vaccination and treatment guidelines.
Compound Chinese medicine (F1) improves spleen deficiency diarrhea by protecting the intestinal mucosa and regulating the intestinal flora
Authors: Wang Kang , Li Guanzong , Yang Zhi , Yang Fumei , Sun Yulin , Duan Gang , Sun Wang , Zhou Ke , He Jun , Dai Feiyan
Published: January 2024

Compound Chinese medicine (F1) is a traditional prescription in Chinese medicine that is commonly used to treat spleen deficiency diarrhea (SDD). It has demonstrated remarkable effectiveness in clinical practice. However, the precise mechanism by which it exerts its antidiarrheal effect is still unclear. This study aimed at investigating the antidiarrheal efficacy and mechanism of F1 on senna-induced secretory diarrhea (SDD). Senna was utilized to induce the development of a mouse model of senna-induced secretory diarrhea (SDD) in order to observe the rate of diarrhea, diarrhea index, blood biochemistry, and histopathological changes in the small intestine. Additionally, the levels of sodium and hydrogen exchange protein 3 (NHE3) and short-chain fatty acids (SCFAs) were determined using enzyme-linked immunosorbent assay (ELISA). The impact of F1 on the senna-induced SDD mouse models was evaluated by monitoring changes in the gut microbiota through 16S rRNA (V3-V4) sequencing. The results demonstrated that F1, a traditional Chinese medicine, effectively increased the body weight of SDD mice and reduced the incidence of diarrhea and diarrhea index. Additionally, F1 restored liver and kidney function, reduced the infiltration of inflammatory cells in intestinal tissue, and promoted the growth of intestinal villi. Furthermore, F1 was found to enhance the expression of NHE3 and SCFAs. It also increased the abundance of Firmicutes and Lactobacillus species, while decreasing the abundance of Proteobacteria and Shigella.
Spermidine improves the antioxidant capacity and morphology of intestinal tissues and regulates intestinal microorganisms in Sichuan white geese
Authors: Wang Zelong , Jiang Dongmei , Wang Xin , Jiang Yilong , Sun Qian , Ling Weikang , An Xiaoguang , Ji Chengweng , Li Shuo , Qi Yuxin , Kang Bo
Published: January 2024

Introduction

Intestinal health is very important to the health of livestock and poultry, and is even a major determining factor in the performance of livestock and poultry production. Spermidine is a type of polyamine that is commonly found in a variety of foods, and can resist oxidative stress, promote cell proliferation and regulate intestinal flora.

Methods

In this study, we explored the effects of spermidine on intestinal health under physiological states or oxidative stress conditions by irrigation with spermidine and intraperitoneal injection of 3-Nitropropionic acid (3-NPA) in Sichuan white goose.

Results and discussion

Our results showed that spermidine could increase the ratio of intestinal villus to crypt and improve intestinal morphology. In addition, spermidine can also reduce malondialdehyde (MDA) accumulation caused by 3-NPA by increasing superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) enzyme activity, thus alleviating intestinal damage. Furthermore, spermidine can regulate intestinal digestive enzyme activities and affect intestinal digestion and absorption ability. Spermidine can also promote an increase in intestinal microbial diversity and abundance and alleviate the change of microflora structure caused by 3-NPA. In conclusion, spermidine promotes the production of beneficial intestinal metabolites such as Wikstromol, Alpha-bisabolol and AS 1–5, thus improving the level of intestinal health. Taken together, these results indicate that spermidine can improve intestinal health by improving intestinal morphology, increasing antioxidant capacity and regulating intestinal flora structure.
UV radiation and temperature increase alter the PSII function and defense mechanisms in a bloom-forming cyanobacterium Microcystis aeruginosa
Authors: Yan Fang , Li Mingze , Zang Shasha , Xu Zhiguang , Bao Menglin , Wu Hongyan
Published: January 2024

The aim was to determine the response of a bloom-forming Microcystis aeruginosa to climatic changes. Cultures of M. aeruginosa FACHB 905 were grown at two temperatures (25°C, 30°C) and exposed to high photosynthetically active radiation (PAR: 400–700 nm) alone or combined with UVR (PAR + UVR: 295–700 nm) for specified times. It was found that increased temperature enhanced M. aeruginosa sensitivity to both PAR and PAR + UVR as shown by reduced PSII quantum yields (Fv/Fm) in comparison with that at growth temperature (25°C), the presence of UVR significantly exacerbated the photoinhibition. M. aeruginosa cells grown at high temperature exhibited lower PSII repair rate (Krec) and sustained nonphotochemical quenching (NPQs) induction during the radiation exposure, particularly for PAR + UVR. Although high temperature alone or worked with UVR induced higher SOD and CAT activity and promoted the removal rate of PsbA, it seemed not enough to prevent the damage effect from them showing by the increased value of photoinactivation rate constant (Kpi). In addition, the energetic cost of microcystin synthesis at high temperature probably led to reduced materials and energy available for PsbA turnover, thus may partly account for the lower Krec and the declination of photosynthetic activity in cells following PAR and PAR + UVR exposure. Our findings suggest that increased temperature modulates the sensitivity of M. aeruginosa to UVR by affecting the PSII repair and defense capacity, thus influencing competitiveness and abundance in the future water environment.
Cannabis use disorder contributes to cognitive dysfunction in Veterans with traumatic brain injury
Authors: Esmaeili Aryan , Dismuke-Greer Clara , Pogoda Terri K. , Amuan Megan E. , Garcia Carla , Del Negro Ariana , Myers Maddy , Kennedy Eamonn , Cifu David , Pugh Mary Jo
Published: January 2024

Background

While emerging evidence supports a link between traumatic brain injury (TBI) and progressive cognitive dysfunction in Veterans, there is insufficient information on the impact of cannabis use disorder (CUD) on long-term cognitive disorders. This study aimed to examine the incidences of cognitive disorders in Veterans with TBI and CUD and to evaluate their relationship.

Methods

This retrospective cohort study used the US Department of Veterans Affairs and Department of Defense administrative data from the Long-term Impact of Military-Relevant Brain Injury Consortium-Chronic Effects of Neurotrauma Consortium Phenotype study. Diagnoses suggesting cognitive disorders after a TBI index date were identified using inpatient and outpatient data from 2003 to 2022. We compared the differential cognitive disorders incidence in Veterans who had the following: (1) no CUD or TBI (control group), (2) CUD only, (3) TBI only, and (4) comorbid CUD+TBI. Kaplan-Meier analyses were used to estimate the overall cognitive disorders incidence in the above study groups. The crude and adjusted Cox proportional hazards models were used to estimate crude and adjusted hazard ratios (HRs) for cognitive disorders.

Results

A total of 1,560,556 Veterans [82.32% male, median (IQR) age at the time of TBI, 34.51 (11.29) years, and 61.35% white] were evaluated. The cognitive disorder incidence rates were estimated as 0.68 (95% CI, 0.62, 0.75) for CUD only and 1.03 (95% CI, 1.00, 1.06) for TBI only per 10,000 person-months of observations, with the highest estimated cognitive disorder incidence observed in participants with both TBI and CUD [1.83 (95% CI, 1.72, 1.95)]. Relative to the control group, the highest hazard of cognitive disorders was observed in Veterans with CUD+TBI [hazard ratio (HR), 3.26; 95% CI, 2.91, 3.65], followed by those with TBI only (2.32; 95 CI%, 2.13, 2.53) and with CUD (1.79; 95 CI%, 1.60, 2.00). Of note, in the CUD only subgroup, we also observed the highest risk of an early onset cognitive disorder other than Alzheimer's disease and Frontotemporal dementia.

Discussion

The results of this analysis suggest that individuals with comorbid TBI and CUD may be at increased risk for early onset cognitive disorders, including dementia.
Targeted therapeutic hypothermia protects against noise induced hearing loss
Authors: Rincon Sabatino Samantha , Rivero Andrea , Sangaletti Rachele , Dietrich W. Dalton , Hoffer Michael E. , King Curtis S. , Rajguru Suhrud M.
Published: January 2024

Introduction

Exposure to occupational or recreational loud noise activates multiple biological regulatory circuits and damages the cochlea, causing permanent changes in hearing sensitivity. Currently, no effective clinical therapy is available for the treatment or mitigation of noise-induced hearing loss (NIHL). Here, we describe an application of localized and non-invasive therapeutic hypothermia and targeted temperature management of the inner ear to prevent NIHL.

Methods

We developed a custom-designed cooling neck collar to reduce the temperature of the inner ear by 3–4°C post-injury to deliver mild therapeutic hypothermia.

Results

This localized and non-invasive therapeutic hypothermia successfully mitigated NIHL in rats. Our results show that mild hypothermia can be applied quickly and safely to the inner ear following noise exposure. We show that localized hypothermia after NIHL preserves residual hearing and rescues noise-induced synaptopathy over a period of months.

Discussion

This study establishes a minimally-invasive therapeutic paradigm with a high potential for rapid translation to the clinic for long-term preservation of hearing health.
Speaker-turn aware diarization for speech-based cognitive assessments
Authors: Xu Sean Shensheng , Ke Xiaoquan , Mak Man-Wai , Wong Ka Ho , Meng Helen , Kwok Timothy C. Y. , Gu Jason , Zhang Jian , Tao Wei , Chang Chunqi
Published: January 2024

Introduction

Speaker diarization is an essential preprocessing step for diagnosing cognitive impairments from speech-based Montreal cognitive assessments (MoCA).

Methods

This paper proposes three enhancements to the conventional speaker diarization methods for such assessments. The enhancements tackle the challenges of diarizing MoCA recordings on two fronts. First, multi-scale channel interdependence speaker embedding is used as the front-end speaker representation for overcoming the acoustic mismatch caused by far-field microphones. Specifically, a squeeze-and-excitation (SE) unit and channel-dependent attention are added to Res2Net blocks for multi-scale feature aggregation. Second, a sequence comparison approach with a holistic view of the whole conversation is applied to measure the similarity of short speech segments in the conversation, which results in a speaker-turn aware scoring matrix for the subsequent clustering step. Third, to further enhance the diarization performance, we propose incorporating a pairwise similarity measure so that the speaker-turn aware scoring matrix contains both local and global information across the segments.

Results

Evaluations on an interactive MoCA dataset show that the proposed enhancements lead to a diarization system that outperforms the conventional x-vector/PLDA systems under language-, age-, and microphone-mismatch scenarios.

Discussion

The results also show that the proposed enhancements can help hypothesize the speaker-turn timestamps, making the diarization method amendable to datasets without timestamp information.
Primary hepatic squamous cell carcinoma: case report and systematic review of the literature
Authors: Zhao Lin , Zhou Yan , Ding Jianmin , Qin Zhengyi , Zhou Hongyu , Jing Xiang
Published: January 2024

Primary hepatic squamous cell carcinoma (SCC) is extremely rare, and only a few dozen cases have been reported to date. It can barely be diagnosed before histopathological examination, which necessitates the exclusion of metastatic tumors. In this case, we present a 60-year-old female patient with no comorbidity. As laboratory tests and imaging examinations were not diagnostic, ultrasonography (US)-guided liver biopsy was performed and eventually revealed a definitive pathological diagnosis of hepatic SCC. After excluding metastasis, the diagnosis of primary hepatic SCC was established, and then chemotherapy and immunotherapy were performed. Additionally, a comprehensive literature search was conducted on primary hepatic SCC using PubMed, Google Scholar, and Web of Science, and a total of 53 articles were retrieved with a time range from 1972 to 2022. A critical analysis was then performed to evaluate previous literature focusing on the clinical characteristics, imaging features, treatments, and prognosis.
Machine learning-based CT texture analysis in the differentiation of testicular masses
Authors: Hu Can , Qiao Xiaomeng , Xu Zhenyu , Zhang Zhiyu , Zhang Xuefeng
Published: January 2024

Purpose

To evaluate the ability of texture features for distinguishing between benign and malignant testicular masses, and furthermore, for identifying primary testicular lymphoma in malignant tumors and identifying seminoma in testicular germ cell tumors, respectively.

Methods

We retrospectively collected 77 patients with an abdominal and pelvic enhanced computed tomography (CT) examination and a histopathologically confirmed testicular mass from a single center. The ROI of each mass was split into two parts by the largest cross-sectional slice and deemed to be two samples. After all processing steps, three-dimensional texture features were extracted from unenhanced and contrast-enhanced CT images. Excellent reproducibility of texture features was defined as intra-class correlation coefficient ≥0.8 (ICC ≥0.8). All the groups were balanced via the synthetic minority over-sampling technique (SMOTE) method. Dimension reduction was based on pearson correlation coefficient (PCC). Before model building, minimum-redundancy maximum-relevance (mRMR) selection and recursive feature elimination (RFE) were used for further feature selection. At last, three ML classifiers with the highest cross validation with 5-fold were selected: autoencoder (AE), support vector machine(SVM), linear discriminant analysis (LAD). Logistics regression (LR) and LR-LASSO were also constructed to compare with the ML classifiers.

Results

985 texture features with ICC ≥0.8 were extracted for further feature selection process. With the highest AUC of 0.946 (P <0.01), logistics regression was proved to be the best model for the identification of benign or malignant testicular masses. Besides, LR also had the best performance in identifying primary testicular lymphoma in malignant testicular tumors and in identifying seminoma in testicular germ cell tumors, with the AUC of 0.982 (P <0.01) and 0.928 (P <0.01), respectively.

Conclusion

Until now, this is the first study that applied CT texture analysis (CTTA) to assess the heterogeneity of testicular tumors. LR model based on CTTA might be a promising non-invasive tool for the diagnosis and differentiation of testicular masses. The accurate diagnosis of testicular masses would assist urologists in correct preoperative and perioperative decision making.
A guide on transnasal endoscopy: setting up a pediatric unsedated endoscopy program
Authors: Friedlander Joel A. , Leinwand Kristina , Bhardwaj Vrinda , Nguyen Nathalie
Published: January 2024

Background

Unsedated transnasal endoscopy is becoming an increasingly popular option for the evaluation of upper gastrointestinal tract disorders in adults and children worldwide. This innovative technology has transformative potential as it provides for a more efficient, safe, and cost-effective method for endoscopy and reduces the risks associated with anesthesia, which is particularly relevant in pediatrics as endoscopy is commonly done under general anesthesia or conscious sedation. The aim is to address knowledge gaps amongst pediatric gastroenterologists who may be considering the development of a TNE program, detailing how to implement sedation-free TNE into practice for pediatric patients and current and forthcoming technologies.

Methods

We conducted a comprehensive review of current literature and collection of data from experts and clinicians in the field on how sedation-free programs were started and being conducted. We aimed to collate the data to provide a guide to address knowledge gaps with a focus on setting up and starting a sedation-free endoscopy program.

Results

Here in, we provide a detailed guide for implementing a sedation-free endoscopy program in pediatrics including design and layout of a TNE unit, special staffing needs, equipment, current and forthcoming technologies, financial considerations and training considerations. We highlight special considerations that are relevant in pediatrics incorporating distraction or dissociation techniques such as Virtual Reality Systems, developmentally appropriate preparation for children, and topical analgesia.

Conclusion

Sedation-free endoscopy is a rapidly growing option for pediatric patients. Development of an unsedated pediatric endoscopy program will improve patient care, decrease the need for anesthesia, provide a lower cost and safe alternative to traditional sedated endoscopy, and is a viable component to a pediatric gastroenterology practice.
Association between mechanical ventilation parameters and mortality in children with respiratory failure on ECMO: a systematic review and meta-analysis
Authors: Fernandez-Sarmiento Jaime , Perez Maria Camila , Bustos Juan David , Acevedo Lorena , Sarta-Mantilla Mauricio , Guijarro Jennifer , Santacruz Carlos , Pardo Daniel Felipe , Castro Daniel , Rosero Yinna Villa , Mulett Hernando
Published: January 2024

Background

In refractory respiratory failure (RF), extracorporeal membrane oxygenation (ECMO) is a salvage therapy that seeks to reduce lung injury induced by mechanical ventilation. The parameters of optimal mechanical ventilation in children during ECMO are not known. Pulmonary ventilatory management during this therapy may impact mortality. The objective of this study was to evaluate the association between ventilatory parameters in children during ECMO therapy and in-hospital mortality.

Methods

A systematic search of PubMed/MEDLINE, Embase, Cochrane, and Google Scholar from January 2013 until May 2022 (PROSPERO 450744), including studies in children with ECMO-supported RF assessing mechanical ventilation parameters, was conducted. Risk of bias was assessed using the Newcastle-Ottawa scale; heterogeneity, with absence <25% and high >75%, was assessed using I2. Sensitivity and subgroup analyses using the Mantel-Haenszel random-effects model were performed to explore the impact of methodological quality on effect size.

Results

Six studies were included. The median age was 3.4 years (IQR: 3.2–4.2). Survival in the 28-day studies was 69%. Mechanical ventilation parameters associated with higher mortality were a very low tidal volume ventilation (<4 ml/kg; OR: 4.70; 95% CI: 2.91–7.59; p < 0.01; I2: 38%), high plateau pressure (mean Dif: −0.70 95% CI: −0.18, −0.22; p < 0.01), and high driving pressure (mean Dif: −0.96 95% CI: −1.83, −0.09: p = 0.03). The inspired fraction of oxygen (p = 0.09) and end-expiratory pressure (p = 0.69) were not associated with higher mortality. Patients who survived had less multiple organ failure (p < 0.01).

Conclusion

The mechanical ventilation variables associated with higher mortality in children with ECMO-supported respiratory failure are high plateau pressures, high driving pressure and very low tidal volume ventilation. No association between mortality and other parameters of the mechanical ventilator, such as the inspired fraction of oxygen or end-expiratory pressure, was found.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023450744, PROSPERO 2023 (CRD42023450744).
A glycosylation-related gene signature predicts prognosis, immune microenvironment infiltration, and drug sensitivity in glioma
Authors: Yang Yanbo , Teng Haiying , Zhang Yulian , Wang Fei , Tang Liyan , Zhang Chuanpeng , Hu Ziyi , Chen Yuxuan , Ge Yi , Wang Zhong , Yu Yanbing
Published: January 2024

Glioma represents the most common primary cancer of the central nervous system in adults. Glycosylation is a prevalent post-translational modification that occurs in eukaryotic cells, leading to a wide array of modifications on proteins. We obtained the clinical information, bulk RNA-seq data, and single-cell RNA sequencing (scRNA-seq) from The Cancer Genome Atlas (TCGA), Chinese Glioma Genome Atlas (CGGA), Gene Expression Omnibus (GEO), and Repository of Molecular Brain Neoplasia Data (Rembrandt) databases. RNA sequencing data for normal brain tissues were accessed from the Genotype-Tissue Expression (GTEx) database. Then, the glycosylation genes that were differentially expressed were identified and further subjected to variable selection using a least absolute shrinkage and selection operator (LASSO)-regularized Cox model. We further conducted enrichment analysis, qPCR, nomogram, and single-cell transcriptome to detect the glycosylation signature. Drug sensitivity analysis was also conducted. A five-gene glycosylation signature (CHPF2, PYGL, GALNT13, EXT2, and COLGALT2) classified patients into low- or high-risk groups. Survival analysis, qPCR, ROC curves, and stratified analysis revealed worse outcomes in the high-risk group. Furthermore, GSEA and immune infiltration analysis indicated that the glycosylation signature has the potential to predict the immune response in glioma. In addition, four drugs (crizotinib, lapatinib, nilotinib, and topotecan) showed different responses between the two risk groups. Glioma cells had been classified into seven lines based on single-cell expression profiles. The five-gene glycosylation signature can accurately predict the prognosis of glioma and may offer additional guidance for immunotherapy.
Anti-inflammatory, analgesic, and antipyretic potential of Oxystelma esculentum (L. f.) Sm. using in vitro, in vivo, and in silico studies
Authors: Sherif Asmaa E. , Sajid-ur-Rehman Muhammad , Asif Muhammad , Qadeer Iram , Khan Kashif ur Rehman
Published: January 2024

The objective of the current study was to evaluate the anti-inflammatory, analgesic, and antipyretic potential of Oxystelma esculentum using different animal models. The phytochemical profile was determined by assessing its total phenolic content (TPC) and total flavonoid content (TFC), followed by the high-performance liquid chromatography (HPLC) technique. The in vitro anti-inflammatory potential of O. esculentum ethanolic extract (OEE) was evaluated by lipoxygenase enzyme inhibition activity and a human red blood cell (HRBC) membrane stability assay. The in vivo anti-inflammatory potential of the plant was determined by the carrageenan-induced paw edema test, and the analgesic potential by the hot plate test, tail-flick test, formalin-induced analgesia, acetic acid-induced writhing activities, and yeast-induced elevation of body temperature. The values of total phenolic content (212.6 ± 3.18 µg GAE/g) and total flavonoid content (37.6 ± 1.76 µg QE/g) were observed. The results showed that OEE exhibited significant antioxidant capacity in DPPH (2,2-diphenyl-1-picrylhydrazyl) (266.3 ± 7.35 μmol TE/g), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (1,066.3 ± 7.53 μmol TE/g), and FRAP (ferric reducing antioxidant power) (483.6 ± 3.84 μmol TE/g) assays. The HPLC analysis demonstrated phytocompounds with anti-inflammatory potential, such as chlorogenic acid, gallic acid, 4-hydroxybenzoic acid, caffeic acid, ferulic acid, and coumarin. The plant showed in vitro anti-inflammatory activity through the inhibition of lipoxygenase enzyme with a high percentage (56.66%) and HRBC membrane stability (67.29%). In in vivo studies, OEE exhibited significant (p < 0.05) anti-inflammatory (carrageenan-induced paw edema model), analgesic (hot plate test, tail-flick test, formalin-induced analgesia, and acetic acid-induced writhing), and antipyretic (rectal temperature reduction) responses at different doses (100, 300, and 500 mg/kg). Molecular docking studies showed significant binding affinities of phytocompounds compared to indomethacin and predicted various binding interactions for stable conformations. The results of in vitro, in vivo, and in silico studies supported the anti-inflammatory, analgesic, and antipyretic potential of O. esculentum.
Ferroptosis: a new mechanism of traditional Chinese medicine for cancer treatment
Authors: Zhu Jiahao , Shen Peipei , Xu Yu , Zhang Xiaojun , Chen Qingqing , Gu Ke , Ji Shengjun , Yang Bo , Zhao Yutian
Published: January 2024

Ferroptosis, distinct from apoptosis, is a novel cellular death pathway characterized by the build-up of lipid peroxidation and reactive oxygen species (ROS) derived from lipids within cells. Recent studies demonstrated the efficacy of ferroptosis inducers in targeting malignant cells, thereby establishing a promising avenue for combating cancer. Traditional Chinese medicine (TCM) has a long history of use and is widely used in cancer treatment. TCM takes a holistic approach, viewing the patient as a system and utilizing herbal formulas to address complex diseases such as cancer. Recent TCM studies have elucidated the molecular mechanisms underlying ferroptosis induction during cancer treatment. These studies have identified numerous plant metabolites and derivatives that target multiple pathways and molecular targets. TCM can induce ferroptosis in tumor cells through various regulatory mechanisms, such as amino acid, iron, and lipid metabolism pathways, which may provide novel therapeutic strategies for apoptosis-resistant cancer treatment. TCM also influence anticancer immunotherapy via ferroptosis. This review comprehensively elucidates the molecular mechanisms underlying ferroptosis, highlights the pivotal regulatory genes involved in orchestrating this process, evaluates the advancements made in TCM research pertaining to ferroptosis, and provides theoretical insights into the induction of ferroptosis in tumors using botanical drugs.
Multivariate generalized mixed-effects models for screening multiple adverse drug reactions in spontaneous reporting systems
Authors: Gosho Masahiko , Ishii Ryota , Ohigashi Tomohiro , Maruo Kazushi
Published: January 2024

Introduction: For assessing drug safety using spontaneous reporting system databases, quantitative measurements, such as proportional reporting rate (PRR) and reporting odds ratio (ROR), are widely employed to assess the relationship between a drug and a suspected adverse drug reaction (ADR). The databases contain numerous ADRs, and the quantitative measurements need to be calculated by performing the analysis multiple times for each ADR. We proposed a novel, simple, and easy-to-implement method to estimate the PRR and ROR of multiple ADRs in a single analysis using a generalized mixed-effects model for signal detection.

Methods: The proposed method simultaneously analyzed the association between any drug and numerous ADRs, as well as estimated the PRR and ROR for a specific combination of drugs and suspected ADRs. Furthermore, the proposed method was applied to detect drug-drug interactions associated with the concurrent use of two or more drugs.

Results and discussion: In our simulation studies, the false-positive rate and sensitivity of the proposed method were similar to those of the traditional PRR and ROR. The proposed method detected known ADRs when applied to the Food and Drug Administration Adverse Event Reporting System database. As an important advantage, the proposed method allowed the simultaneous evaluation of several ADRs using multiple drugs.