bluuebunny/crossref_metadata_2025 huggingface dataset:
Whole Exome Sequencing on a multiplex family of Indian origin identifies mutations at 17p11.2 in siblings with autism spectrum disorders: implications to understanding the pathophysiology from syndromic variants.
Authors: Srivid Durbagula , Ranganayaki Sathyanarayanan , Parambath Snijesh Valiya , S Aparna H , Korlimarla Aruna , Prabhakaran Nishant , Vijayanand Meghana , Gowda Naveen Kumar C
Published: October 2023

Autism Spectrum Disorders (ASDs) is a complex neurodevelopmental disorder characterized by restrictive repetitive behaviour and impairment in social and communication skills. It is extremely heterogeneous with a strong genetic preponderance. It’s clinically highly convoluted, represented with multiple comorbid conditions and syndromic features. More than 100 genes have been identified till date that are associated with syndromic ASDs. Whole exome sequencing (WES) has emerged as a valuable tool in evaluating the genetic underpinnings of ASDs, be it the syndromic or the idiopathic variants. The current study leverages the utility of WES in a multiplex family of Indian origin to identify the disease etiology in the siblings (01S1 (Female) and 01S2 (Male)) exhibiting ASD syndromic features, at both clinical and molecular levels. Our systematic bioinformatic analysis identified a missense mutation (NM_030665.4:c.5320C > T; p.Arg1774Trp) in 01S1 resulting in RAI1 haploinsufficiency. Validation by sanger sequencing confirmed the novelty of the said mutation and that it was true positive and maternally transmitted in the subject. Likewise, we report a missense mutation at the same locus (17p11.2) corresponding to the FLII gene (NM_002018.4:c.2030A > C;p.Glu677Ala) in the other sibling, 01S2. Both the mutations were reported in the Smith Magenis Syndrome (SMS) critical region justifying its contribution to the presentation of the syndromic SMS features. These WES findings were consistent with the clinical findings that imply SMS features in both siblings. The current study employs WES to provide insights into the complex molecular mechanism associated with syndromic ASD highlighting the different processes that contribute to the disease heterogeneity. Moving forward, combinatorial approaches and findings from syndromic ASDs can potentially act as indicators to understand the genetic and phenotypic variations seen in the idiopathic ASD.
Synthesis and studies on some cholesterol containing poly-siloxane based side- chain liquid crystal elastomers
Authors: Mohana K. , Deepa M. , Umadevi S.
Published: October 2023

Siloxane-based side-chain liquid crystal elastomers (SCLCEs) containing cholesteryl 4-(undec-10-en-1-yloxy)benzoate mesogenic unit are described. The cholesteric mesogen was dimesomorphic and displayed a smectic A and nematic phases having a phase range of 69°C and 32°C respectively. Four elastomers (polydomain powders) were synthesized by employing the LC mesogen, polymethylhydrosiloxane (PMHS) backbone and four different cross-linkers (alkyl, phenyl, biphenyl and azo) through hydrosilylation reaction. All the elastomers were found to be stable up to 300°C and exhibited an intercalated lamellar structure. Among the four elastomers studied, a wide phase range was observed for LCE4 with an azo cross-linker and the LCE1 containing an alkyl cross-linker exhibited a low phase range. The photoisomerization of the LCE4 (due to the presence of azo- group) was investigated in toluene and photostationary state for trans-to-cis conversion was observed at 150 s. The polymeric films of the elastomers were also prepared. A noticeable bending response was was observed for a thermal stimulus in elastomers containing alkyl, phenyl and azo cross-linkers which was more pronounced in LCE4 having azo cross-linker.
Integrated Bioinformatics and Machine Learning Algorithms of Identify Novel Diagnostic Biomarkers and Chinese Medicine Targets in Chronic Atrophic Gastritis
Authors: Weng Jiao , Wu Xiu-fang , Shao Peng , Liu Xing-pu , Wang Cai-xia
Published: October 2023

Chronic atrophic gastritis (CAG) serves as one of the warning signals for gastric cancer, and the utilization of traditional Chinese medicinal herbs brings hope for patients' recovery. In this study, a combination of bioinformatics and machine learning algorithms was employed to explore the precise targeting of CAG diagnostic biomarkers based on meta-analysis of key Chinese herbal formulas. Firstly, through integrating transcriptomic samples from normal gastric tissue and CAG tissue from three datasets (GSE116312, GSE27411, and GSE54129), differentially expressed genes (DEGs) were identified. Further functional and pathway analysis of the DEGs was performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Twelve Chinese herbal formulas were obtained through meta-analysis, and seven hub herbs were identified through association rule mining, namely, White Peony Root (Baishao), Largehead Atractylodes Rhizome (Baizhu), Pinellia Tuber (Banxia), Tangerine Peel (Chenpi), Root of Pilose Asiabell (Dangshen), Danshen Root (Danshen), and Coptis Root (Huanglian). A total of 248 target genes were associated with these medicinal herbs. Among the 905 CAG-related disease genes retrieved from five databases, 90 target genes of Chinese medicine (TCMTGs) were found to be shared with hub genes that are both pivotal and CAG-related. The regulatory network of Chinese medicine target genes and protein-protein interaction (PPI) network of target genes were constructed to observe the underlying mechanisms. Ten hub genes (BCL2L1, MAPK3, RASSF1, GSTP1, CCND1, CAT, MET, MMP3, THBD, and MAPK1) were identified from the intersection of DEGs and TCMTGs. Through gene correlation, sample expression levels, chromosomal positions, transcription factors, PPI networks, GO, and KEGG enrichment analysis, the characteristics of these hub genes were explored. By utilizing four machine learning algorithms – support vector machine (SVM), generalized linear model (GLM), decision tree model, and K-Nearest Neighbors model – diagnostic biomarkers for CAG (MET, MAPK1, and GSTP1) were obtained. The receiver operating characteristic (ROC) curves, nomogram plots, calibration curves, and clinical decision curves were constructed to evaluate the models. Finally, molecular docking was conducted between three protein receptors (MET-P08581, MAPK1-Q9H706, and GSTP1-P09211) and four active small molecular ligands of Chinese herbs (luteolin, naringenin, quercetin and kaempferol). In summary, the integration of machine learning models with bioinformatics methods for screening drug-targeting gene markers not only elucidated the mechanisms of active compounds in traditional Chinese medicine but also provided support for new drug development, thus increasing the potential to interrupt the progression of CAG into gastric cancer.
Comprehensive analysis of the distinct nano environments characteristics containing the different secondary structure elements: α- helices, β-sheets, and turns
Authors: Mazoni Ivan , Salim José Augusto , Moraes Fabio Rogerio , Correa Jorge Luiz , Borro Luiz , Neshich Goran
Published: October 2023

This work is the third part of our initiative to fully describe the internal protein nano environments (NEs) for the three existing types of secondary structure elements (SSE). In our previous work, the NE of both the α-helix and the β-sheet were analysed. The knowledge acquired in this research is important considering that secondary structure element formation is a crucial step in protein folding and an important phase that precedes the final 3D protein structure. In the current paper, STING´s database of physical-chemical and structural descriptors was used to gather the necessary information to characterize the NE of loops, or, as they are often called, turns. Given that approximately 20% of all protein-type residues form turns, research in this field is essential, and analysis of the obtained results will further contribute to our comprehension of how proteins fold. In addition, the results in this paper will contribute to the better training of algorithms that evaluate the degree of overall protein structure quality and, consequently, structure prediction. This is currently very important given we are witnessing a revolution in algorithms employing artificial intelligence for protein structure prediction. Powered by the STING’s database (wide-ranging protein structure information source), statistical testing was used to retrieve a set of descriptors that fully delineate the NE of turns. By collecting such data, it is then possible to list the variances with respect to the NE of α-helices and β-sheets and, by doing so, establish the most relevant NE descriptors (MRND) for each of the three SSEs. The results show that the α-helical and β-sheet Nes, as well as the amino acid residue composition, all behave in a similar fashion as a “key and lock” system. In other words, it is necessary for a set of specific descriptors to assume respective specific values (within the bounds of a very definite value region) to construct the specific secondary structure element NE at a certain protein location. Consequently, there is a set of descriptors that act together that are required to satisfy specific conditions for secondary structure element occurrences. The very same requirement, we found, occurs in the case of turns.
Anti-tumor Efficacy and Potential Mechanism of Pyrotinib in Locally Advanced Oral Squamous Cell Carcinoma
Authors: Zhou Liang , Wang Huiming , Chen Qianming , Le Kehao
Published: October 2023

Background
The resistance to epidermal growth factor receptor (EGFR) target therapy is common in advanced oral squamous cell carcinoma (OSCC). Meanwhile human epidermal growth factor receptor 2 (HER2) plays an important role in the progression of multiple solid tumors and induces resistance to EGFR target treatment. However, the expression status and the clinical significance of HER2 in OSCC is still controversial. Pyrotinib has shown promising activity as a novel EGFR/HER2 dual inhibitor, in many advanced cancers, but its efficacy in OSCC has not been determined.
Methods
57 locally advanced de novo OSCC patients admitted into a single tertiary referral hospital were enrolled in this study with the approval of the ethics committee. Through tissue microarray analysis of the primary tumors and paired para-tumor oral mucosa, the relationship between the expression levels of HER2 and the prognosis of OSCC patients had been investigated. To complement these findings, the antitumor efficacy of pyrotinib in OSCC was retrieved in vitro and in vivo. The main downstream of HER2 was evaluated by western blotting in OSCC cell lines and xenograft tumors to explore the potential mechanism of pyrotinib.
Results
This study revealed the primary tumor of OSCC had higher HER2 expression levels. Through Kaplan-Meier analysis, OSCC patients with high HER2 expression had poor overall survival (P < 0.014) and poor disease free survival (P < 0.042). In vitro, pyrotinib suppressed the proliferation, colony formation and migration of OSCC cells. Pyrotinib also promoted apoptosis of OSCC cells and induced cell cycle arrest. This study also confirmed that pyrotinib was able to inhibit the occurrence and development of OSCC effectively in vivo. Furthermore, western blotting revealed that pyrotinib suppressed OSCC by inhibiting the phosphorylation of HER2, AKT and ERK in vitro and in vivo.
Conclusions
This is the first study to exhibit the anti-OSCC effects of pyrotinib in vitro and in vivo, and demonstrated pyrotinib inhibited OSCC cells by inducing apoptosis via the HER2/ AKT and ERK pathway. The result of this study also indicated locally advanced OSCC patients might benefit from HER2 assay and EGFR/HER2 dual inhibit treatment.
Evaluating the Salivary Levels of Vitamins A, B12, C, D3, E in Oral Lichen Planus Patients in Southwest of Iran
Authors: Rezazadeh Fahimeh , Abedi Fatemeh , Ranjbar Zahra , Khorami Elham Tayebi , Tabesh Amir
Published: October 2023

Background
The present study aims to assess salivary levels of vitamins A, B12, C, D3 and E and compare them with a healthy group.
Methods
58 individuals, attending Shiraz Dental School, Iran, were enrolled in this cross-sectional study (28 OLP patients and 30 healthy controls, age and sex matched.) saliva samples were collected and levels of A, B12, C, D3 and E vitamins were measured. HPLC was used for measuring vitamins A, B12 and E and ELISA kits for vitamins C and D.
Results
Our study population’s mean age was 44.964 ± 10.32, including 67% female. Levels of vitamins A, C, D3 and E were lower in the saliva of OLP patients (P = 0.000, 0.000, 0.013 and 0.000 respectively), but that wasn’t the case for vitamin B12 (P = 0.699). Vitamins A, C and E were interrelated to the erosive type of OLP (P = 0.003, 0.000 and 0.000 respectively). OLP occurrence was significantly correlated with the female population, but not with the age of participants.
Conclusion
Salivary levels of vitamins A, C, D3 and E were significantly lower in OLP patients. There was no statistical difference in vitamin B12 levels in the saliva of OLP patients and the healthy control population.
Using ChatGPT to Develop the Statistical Analysis Plan for a Randomized Controlled Trial: A Case Report
Authors: Evans Richard , Pozzi Antonio
Published: October 2023

Background
Statistical analysis plans (SAPs) outline the way data will be collected, preprocessed and analyzed, and should be developed during the design phase of the study. However, veterinary researchers often have limited access to biostatistical resources to develop statistical analysis plans. That access can be improved using natural language artificial intelligence chat services (chatbots), such as ChatGPT, which provide ways to access biostatistical expertise that can be understood by nonstatistician researchers. The aim of this study was to demonstrate a ChatGPT query protocol that produced a statistical analysis plan for randomized controlled trial comparing orthopedic surgical methods.
Implementation
Our overall approach was to interact with the chatbot ChapGPT as we would with a biostatistician planning a clinical trial’s data preprocessing and statistical analysis. Collaborations with biostatisticians are iterative, where the investigator initiates the statistical conversation with an overview of the project, and then the biostatistician and investigator go back and forth to refine the SAP with a series of more detailed questions and responses. Similarly, we iteratively queried ChatGPT after reviewing its responses.
Results
A good SAP was developed after four queries, which took 15 minutes. Researchers with less statistical experience may require more queries and time. ChatGPT produced an acceptable SAP that could be understood by nonstatistician researchers.
Discussion
ChatGPT 3.5 is free, fast, and interactive. Chatbot-generated SAPs might improve the reproducibility of veterinary research because they could be included as supplementary material. Additionally, SAPs are useful for responding to statistical reviewers because SAPs anticipate reviewer questions. Moreover, key analysis elements are often missing from study reports, and SAPs could serve as checklists to ensure that all statistical steps are performed. Finally, even when biostatistical support is available, a chatbot-generated SAP could provide a framework for a biostatistician, and speed up the research process by weeks.
Conclusions
When used iteratively, ChatGPT provides a sound framework for the steps in the statistical analysis of randomized controlled trials. We anticipate that it would be useful for other common study designs, such as risk factor studies, survival analyses, systematic reviews, and observational cohort studies.
Identification and immune landscape of sarcopenia-related molecular clusters in inflammatory bowel disease by machine learning
Authors: Yue Chongkang , Xue Huiping
Published: October 2023

Sarcopenia, a prevalent comorbidity of inflammatory bowel disease(IBD), is characterized by diminished skeletal muscle mass and strength, and it has garnered increasing attention. Nevertheless, the underlying interconnected mechanisms remain elusive. This study pioneered the identification of distinct expression patterns within sarcopenia-related genes (SRGs) across individuals with IBD and in samples of normal tissue. By analyzing various SRG expression profiles, we effectively segregated 860 IBD samples into two distinct clusters, each marked by its unique immune landscape. To unravel the transcriptional disruptions underlying these clusters, the Weighted Gene Co-expression Network Analysis (WGCNA) algorithm was employed to spotlight key genes linked to each cluster. Leveraging machine learning, namely RF, LASSO, and SVM-RFE algorithms, we established a diagnostic model grounded in 13 key genes (LYN, IFITM2, ACSL4, CLEC4E, SOCS3, PLAU, TIMP1, NCF2, MNDA, IL1B, CXCL1, MMP1, and S100A8). Moreover, the GSE112366 dataset facilitated the exploration of gene expression dynamics within the ileum mucosa of UC patients pre- and post-Ustekinumab treatment. Additionally, insights into the intricate relationship between immune cells and these pivotal genes were gleaned from the single-cell RNA (scRNA) dataset GSE162335. In conclusion, our findings collectively underscored the pivotal role of sarcopenia-related genes in the pathogenesis of IBD. Their potential as robust biomarkers for future diagnostic and therapeutic strategies is particularly promising, opening avenues for a deeper understanding and improved management of these interconnected conditions.
Work Pressure, Coping Styles and Occupational Burnout Among Chinese Police Officers: A Meta-analytic Review
Authors: Zhou Senlin , Li Miaomiao , Chen Siru , Jiang Daokui , Qu Ying
Published: October 2023

The present study conducted a comprehensive meta-analysis to systematically review the relationship between occupational burnout and work pressure among Chinese police officers. Additionally, the study explored the mediating role of coping styles using a meta-analytic structural equation model. The investigation involved a thorough search of CNKI, PubMed, PsychInfo, Web of Science, and Google Scholar databases, resulting in the identification of a total of 39 studies with 124 effect sizes and 14089 police officers. The findings revealed a positive correlation between work pressure and occupational burnout among Chinese police officers (r = 0.410, 95% CI = [0.347, 0.469]). Furthermore, negative coping styles mediate the relationship between work pressure and occupational burnout. Importantly, these conclusions held true across various work regions for police officers. These results provide insights into the relationship magnitude between work pressure and occupational burnout in Chinese police work and shed light on the underlying mechanisms. Based on these findings, it is recommended that interventions focusing on reducing work pressure and fostering positive coping styles be implemented to mitigate occupational burnout among police officers.
Decorating phenylalanine side-chains with triple labeled 13 C/ 19 F/ 2 H isotope patterns
Authors: Toscano Giorgia , Holzinger Julian , Nagl Benjamin , Kontaxis Georg , Kählig Hanspeter , Konrat Robert , Lichtenecker Roman J.
Published: October 2023

We present an economic and straightforward method to introduce 13C-19F spin systems into the deuterated aromatic side chains of phenylalanine as reporters for various protein NMR applications. The method is based on the synthesis of [4-13C, 2,3,5,6-2H4] 4-fluorophenylalanine from the commercially available isotope sources [2-13C] acetone and deuterium oxide. This compound is readily metabolized by standard E. coli overexpression in a glyphosate-containing minimal medium, which results in high incorporation rates in the corresponding target proteins.
CD72 Downregulation on CD5+ B Cells Promotes CD5-associated B Cell Proliferation and IL-10 expression during HBV Infection
Authors: Li Bingjie , Zhu Qingfeng , Zhang Yan , Ju Mabaike , Song Ying , Xie Songsong
Published: October 2023

Backgroud:B cells play an important role in HBV infection. CD72 is expressed on B cells, and negatively regulates B cell activation. And the effects of CD72 on B cell proliferation associated with CD5 expression have not yet been studied. The aim was to assess the impact of CD72 expression on CD5+ B cells during HBV infection.
Method: Peripheral blood mononuclear cells with HBV infection and healthy donors were collected, and CD72 on B cells were examined. The correlation between CD72 and CD5 expression on B cells was analysed by flow cytometry. The effect of CD72 expression on signal transduction and B cell proliferation were assessed by western blotting and BrdU assays.
Results: CD72 expression was significantly lower on CD5+ B cells of patients with HBV infection than those of healthy donors (P < 0.001). A negative correlation was found between CD72 and CD5 expression on B cells (P <0.01 in cells%; P = 0.033, in mean fluorescence intensity). CD5+ B cell proliferation was significantly increased after CD72 suppression. CD5+ B cell proliferation was associated with ERK and Syk activation in patients with HBV infection. And the CD72 downregulation in CD5+ B cell induced IL-10 upregulation.
Conclusion: CD72 expression was downregulated in CD5+ B cells of patients with HBV infection. Downregulation of CD72 in CD5+ B cells promotes CD5-associated B cell proliferation and IL-10 expression in an ERK and Syk signalling-dependent manner.
Development of a CRISPR/Cas12a-based fluorescent detection system of Senecavirus A
Authors: He Wei , Liao Kai , Li Ruixue , Peng wanqing , Qian Bingxu , Zeng Dexin , Tang Fang , Xue Feng , Jung Yong sam , Dai jianjun
Published: October 2023

Background Senecavirus A (SVA) was found in 2002 and could cause porcine idiopathic vesicular disease (PIVD) which symptoms were similar to vesicular diseases resulting in increased difficulty of a field diagnosis. However, traditional molecular diagnosis failed to reconcile cost, instrument, sensitivity, and efficiency.
Methods In this study, we integrated pre-amplification and three kinds of sensor systems with CRISPR and therefore established an SVA diagnosis platform with highly adaptable and ultra-sensitive advantages. This diagnosis which the whole process should not exceed four hours consisted of three steps: nucleic acid extraction, pre-amplification, and fluorescent signal capture.
Results This method showed no cross-reaction with other 10 swine viruses; its limit of detection was as low as one copy/reaction of SVA; its accuracy for clinical sample diagnosis was 100%.
Conclusions In addition, this diagnosis used common instruments which would be beneficial to remote testing sites or poorly equipped laboratories to promote a large-scale screening of this epidemic. Overall, this diagnosis enlarged diagnostic tools of SVA and had the potential to play a positive role in the control of PIVD.
The identification of novel biomarkers offers potential avenues for therapeutic interventions in lung adenocarcinoma
Authors: Meng Lifei , Bo Xue , Jun Zhao , Zhou Chengwei , Li Chenwei
Published: October 2023

Background: Lung cancer stands as a prominent contributor to cancer-related fatalities, with lung adenocarcinoma emerging as one of its primary histological subtypes. Regrettably, the 5-year survival rate for patients afflicted by this condition remains below 20%. Mitochondria, being indispensable organelles responsible for upholding cellular functionality, are susceptible to malfunctioning and can consequently give rise to diverse diseases. Henceforth, the objective of this study is to scrutinize the correlation between genes associated with mitochondria and the onset and progression of lung adenocarcinoma tumors .
Method: The lung adenocarcinoma gene expression dataset was obtained from the TCGA database for conducting differential gene expression analysis. Subsequently, Kaplan-Meier survival analysis, univariate and multivariate Cox regression analyses were performed on the differentially expressed genes (DEGs), leading to the construction of a nomogram based on these analytical results. Additionally, GO, KEGG, and GSEA enrichment analyses were carried out on the DEGs.
Results: Differential gene expression analysis revealed the presence of 13 upregulated genes related to mitochondrial fission in lung adenocarcinoma tissues, which were found to be associated with prognosis. After excluding one unfavorable prognostic gene, survival analysis was conducted on the remaining 12 genes. Univariate and multivariate Cox regression analyses demonstrated that N stage, T stage, risk score, and age significantly influenced the prognosis of lung adenocarcinoma. Based on these findings, a nomogram was developed for predicting the survival probability of patients with lung adenocarcinoma at different time points (1 year, 3 years, 5 years, and 10 years). Furthermore, GO (Gene Ontology), KEGG (Kyoto Encyclopedia of Genes and Genomes), and GSEA (Gene Set Enrichment Analysis) enrichment analyses indicated that the differentially expressed genes were predominantly enriched in pathways associated with cellular senescence and cell cycle regulation. These results suggest their potential relevance to tumor development in lung adenocarcinoma.
Conclusion: This study identified 12 differentially expressed genes, namely BIRC5, BRCA1, CA9, CDK1, CERS1, FOXM1, GAPDH, GPI, MTFR2, PPIF, RACGAP1 and S100A9. These genes hold promising potential as prognostic biomarkers for lung adenocarcinoma.
Group Brief Cognitive Behavioral Therapy for Suicide Prevention Compared to Dialectal Behavior Therapy Skills Group for Military Service Members: A Study Protocol of a Randomized Controlled Trial
Authors: Baker Justin C. , Grover Shawna , Gunn Laura H. , Charles Cindy , Rikli Heather , Franks Michael J. , Khazem Lauren R. , Williams Sean , Ammendola Ennio , Washington Cherita , Bennette Marquita , Starkey Austin , Schnecke Kelly , Cain Shannon , Bryan Craig J. , Cramer Robert J.
Published: October 2023

Background
Suicide is a pressing matter for the military. Not only does it pose a health risk, but suicide also compromises operational readiness. Despite provision of suicide prevention clinical best practices, the Department of Defense suffers several challenges (e.g., clinician shortages) limiting the agency’s ability to effectively respond to service member suicide. Implementation of evidence-based suicide-specific group therapy is a possible solution to service member well-being needs and system challenges. Service members can also gain coping skills useful beyond managing suicidal thoughts and behaviors.
Methods
This 2-arm non-inferiority randomized controlled trial compares a group therapy format of Brief Cognitive Behavioral Therapy (i.e., G-BCBT) with Dialectical Behavior Therapy (DBT) Skills Group. Both therapies are delivered in-person at a United States Naval Medical Center. Participants (N = 136) are active-duty service members with recent suicidal thoughts or suicidal behavior. Evaluation features electronically delivered questionnaires at baseline, after each treatment session, and at 3- and 6-month follow-up.
Discussion
The primary outcome concerns G-BCBT impacts on suicidal ideation. Secondary outcomes of interest are suicide attempt, psychological distress (e.g., symptoms of depression, anxiety), and self-regulatory skills (e.g., emotion regulation). We also examine self-regulatory skills as treatment moderators. Clinical trial strengths and limitations are reviewed.
Trial registration
This study was registered at Clinicaltrials.gov (protocol NCT05401838).
Optimising the implementation of digital-enabled interventions for the secondary prevention of heart disease: A systematic review using the RE-AIM planning and evaluation framework
Authors: Moel-Mandel Caroline , Lynch Chris , Issaka Ayuba , Braver Justin , Zisis Georgios , Carrington Melinda J , Oldenburg Brian
Published: October 2023

BackgroundDigital-enabled interventions are widely utilised to support the delivery of programs for the secondary prevention of heart disease. They have shown a similar efficacy and safety to centre-based programs and improvements in access and adherence; the key barriers to in-person programs. However, questions remain regarding the successful wider implementation of digital-enabled programs. By applying the RE-AIM framework to a systematic review and meta-analysis, this review aims to evaluate the extent to which these programs report on RE-AIM dimensions and associated indicators.MethodsThis review extends our previous systematic review and meta-analysis that investigated the effectiveness of digital-enabled programs for coronary artery disease patients after hospitalisation. Citation searches were performed on the 27 studies of the systematic review and meta-analysis to identify linked publications that reported data for RE-AIM dimensions. All included studies and, where relevant, any additional publications, were coded using an adapted RE-AIM extraction tool. Discrepant codes were discussed amongst reviewers to gain consensus. Data were analysed to assess reporting on indicators related to each of the RE-AIM dimensions, and average overall reporting rates for each dimension were calculated.ResultsAcross all 27 studies and nine additional reports, 24 (89%) studies were interventions solely delivered at home. In 11 (46%) of these studies, there were no staff involved in the intervention delivery or staff were only available on request. Ten studies (37%) used a mobile application, and only four (15%) studies did not require access to a smartphone. Across all studies the average reporting rates for RE-AIM dimensions were highest for effectiveness (75%) and reach (67%), followed by adoption (54%), implementation (36%) and maintenance (11%).ConclusionsThrough the application of the RE-AIM framework to a systematic review, we found most studies failed to report on key indicators. Failing to report these indicators inhibits the ability to address the enablers and barriers required to achieve optimal intervention implementation in wider settings and populations. Future studies should consider indicators in design and report on implementation indicators to improve the translation of research evidence into routine practice, with special consideration given to the long-term sustainability of program effects as well as corresponding ongoing costs.Registration:PROSPERO - CRD42022343030
Coupling coordination degree of carbon emissions in production, living and ecology space and influencing factors in the Aksu River Basin
Authors: Han Fanghong , Gao Fan , He Bing , Cao Yan , Liu Kun
Published: October 2023

Inland river basins in arid zones have fragile and sensitive ecological backgrounds but are very important ecological niches. Scientific assessments of the coupling coordination development of carbon emissions in production, living, and ecological spaces (PLES) caused by the expansion of man-made oases in these areas, and exploration of the spatial differentiation patterns and influencing factors of carbon emissions in the PLES, are crucial for optimizing basin territorial space and promoting high-quality green development. The study assessed the coupling coordination degree (CCD) of carbon emissions and its spatial differentiation in the basin's in production, living, and ecological spaces. The identified the factors affecting CCD using spatial autocorrelation analysis, Geographically and Temporally Weighted Regression (GTWR) model, and other spatial statistical tools. The results showed that: In the spatial dimension, CCD had the spatial distribution pattern of "high in the east and low in the west". In the time dimension, the evolution of CCD was characterized by "decreasing first and then rising". Factors such as elevation, slope, temperature, precipitation, and population significantly impacted CCD, and had obvious spatial aggregation. In the time dimension, the influence of population on the CCD was relatively stable, while the influence of other factors on the CCD had different trends.
Experiences and Attitudes Towards Sexual Harassment Among Hawkers Residing in Ede Township in Osun State, Nigeria
Authors: Omoge Adeyemi O. , Adesegun Mobolaji M. , Arogundade Ayorinde O. , Godfrey Sally G. , Obi-Obasi Chiamaka S. , Opadele Modurotolu M.
Published: October 2023

BACKGROUND
Peddling through the streets in developing countries is another area of interest the researchers will love to explore due to the socio-economic and negative health effects that can results from this by those who involved in it. This includes sexual assault which increases the vulnerability of the hawkers to diseases such as HIV/AIDS and other sexually transmitted infections, increased risk of unwanted pregnancies and unsafe abortion.
OBJECTIVE
This study aimed to gain an understanding on the experiences and attitudes towards sexual harassment among hawkers residing in Ede Township in Osun State, Nigeria.
METHODOLOGY:
The study population comprises of hawkers in Ede Township in Ede, Osun State. Fifty respondents were reached through questionnaire titled Experiences and Attitudes towards Sexual Harassment among Hawkers Residing in Ede Township in Osun State, Nigeria. Quantitative research method was applied for data collection. 17 items questionnaire containing 3 segments was used for data collection and SPSS version 23 was used for analysis of data.
RESULTS
The result revealed that 21 (42%) were between the age of 16–20 years, 38 (76%) were females, 42 (84%) were Islam, 49 (98%) were Yoruba of which 39 (78%) were single. Also, 37 (74%) were students, 43 (86%) had secondary school education 14 (28%) earned 1,000–1,900 and 2,000–2,900 per day as income. 16 (32%) responded that sometimes has anyone made gesture or body language of a sexual nature to them, 45 (90%) strongly disagree that they are comfortable with people of opposite sex touching their buttocks without their consent and 44 (88%) strongly disagree that they are comfortable with people of opposite sex touching their waist without their consent.
CONCLUSION
In conclusion, street hawking is a variant and a very visible phenomenon in our society. It is largely due to poverty and underdevelopment. The hawkers are exposed to many risks of which sexual abuse is a prominent component. The government needs to vigorously pursue poverty alleviation measures to improve the general socio-economic status of majority of the population. There are needs to give sex education to all young people with emphasis on risk reduction measures.
Chondrogenesis of Mesenchymal Stromal Cells on the 3D Printed Polycaprolactone/Fibrin/Decellular Cartilage Matrix Hybrid Scaffolds in the Presence of Piascledine
Authors: Honarvar Ali , Setayeshmehr Mohsen , Hashemibeni Batool , Ghaedamini Sho’leh , Moroni Lorenzo , Karbasi Saeed
Published: October 2023

Today, cartilage tissue engineering (CTE) is considered important due to the lack of repair of cartilaginous lesions and the absence of appropriate methods for treatment. In this study, polycaprolactone (PCL) scaffolds were fabricated by three-dimensional (3D) printing and were then coated with fibrin (F) and acellular solubilized extracellular matrix (ECM). After extracting adipose-derived stem cells (ADSCs), 3D-printed scaffolds were characterized and compared to hydrogel groups. After inducing the chondrogenic differentiation in the presence of Piascledine and comparing it with TGF-β3 for 28 days, the expression of genes involved in chondrogenesis (AGG, COLII) and the expression of the hypertrophic gene (COLX) were examined by real-time PCR. The expression of proteins COLII and COLX was also determined by immunohistochemistry. Glycosaminoglycan was measured by toluidine blue staining. 3D-printed scaffolds clearly improved cell proliferation, viability, water absorption, and compressive strength compared to the hydrogel groups. Moreover, the use of compounds such as ECM and Piascledine in the process of ADSCs chondrogenesis induction increased cartilage-specific markers and decreased the hypertrophic marker compared to TGF-β3. In Piascledine groups, the expression of COLL II protein, COLL II and Aggrecan genes, and the amount of glycosaminoglycan showed a statistically significant increase in the PCL/F/ECM compared to the PCL and PCL/F groups (P < 0.05). The results confirmed that the PCL/F/ECM scaffolds presented in this study afford unique opportunities for CTE.
Changes in the shape of carrageenan molecules, from bricks to nanospheres with unique procedures
Authors: Bhatt Akanksha , Khailkhura Shashank , purohit Priyank
Published: October 2023

The interactions between the polymer's surface area and kinetic behaviors make the polymer's size, shape, and related properties of concern to polymer scientists. Polymers, especially when grafted or derivatized to improve their diagnostic, targeted, sustained, or controllable carrying capacities, are a strong selling point. In this investigation, we characterize iota-carrageenan power as it is used to fabricate a brick-shaped and spherical nanoparticle. Our previous work with solvent screening led us to the discovery of the solubility, ionic effect, and desolvation process upon which our newly developed methodology is based. The de-solvating agent's effect was responsible for the smaller particle size. The particles' sizes and shapes were compared to one another and correlated with their potential applications in the pharmaceutical industry. The size progression from powder to nanosphere is difficult, but maintaining the high surface energy (brick shape) bearing shape is more difficult. This is the focus of the current research.
Barbara Antkowiak, 1933–2004
Authors: Richter Angela
Published: October 2023

Im Frühherbst 2004 verstarb Barbara Antkowiak, eine der verdienstvollsten Über­setzerinnen aus den südslawischen Sprachen, unerwartet an einer Lungenembolie. Damit hat der deutschsprachige Kulturkontext eine Vermittlerin verloren, für die die Präsentation von Werken und Autoren aus den slawischen Sprachen Südosteuropas Herzenssache war.
Erich Arendt, 1903–1984
Authors: Vejmelka Marcel
Published: October 2023

Erich Arendt zählt zu den bedeutendsten Übersetzern spanischsprachiger Lyrik ins Deutsche. Seine Übersetzungen von Pablo Neruda und anderen ebneten den Weg für die Rezeption der lateinamerikanischen Dichtung zunächst in der DDR, später dann in der BRD. Auch zentrale Persönlichkeiten der spanischen Dichtung des 20. Jahrhunderts wie Vicente Aleixandre, Rafael Alberti und Miguel Hernández wurden von Arendt ins Deutsche übertragen. Erich Arendt steht exemplarisch für die Figur des "Dichter-Übersetzers".
MODELO DE RESPONSABILIDAD SOCIAL CORPORATIVA PARA LA CORPORACIÓN NACIONAL DE ELECTRICIDAD, AGENCIA JIPIJAPA
Authors: Parrales Reyes Jenny Elizabeth , Soledispa Ventura Kerly Irina
Published: October 2023

El modelo de responsabilidad social corporativa para optimizar la gestión administrativa y empresarial de la Corporación Nacional de Jipijapa se fundamenta en cuatro ejes claves: desarrollo humano, aporte a la comunidad, desempeño ambiental y su eficacia operativa. A través de la implementación de este modelo, la corporación busca generar un impacto positivo tanto a nivel interno como externo, alineando sus operaciones con principios de sostenibilidad y responsabilidad social, este modelo articula demás los objetivos del plan estratégico de CNEL EP y los objetivos de desarrollo sostenible de la ONU, se pretende generar resultados positivos y medibles mediante la utilización de varios indicadores de impacto. Su metodología abarca la recopilación de datos acerca de la situación presente de la planeación estratégica y la gestión administrativa de CNEL Jipijapa, además de la utilización de los métodos cuali-cuantitativos para cuantificar la influencia de la ejecución de este modelo de responsabilidad social corporativa.
CAMPO DE UNA MASA PUNTUAL Y UNA HIPÓTESIS COSMOLÓGICA
Authors: Álvarez Vita Enrique
Published: October 2023

Uno de los problemas de la física en la mecánica cuántica y la relatividad general es el de las singularidades. En la electrodinámica cuántica se ha utilizado con éxito el método de la renormalización, el que sin embargo no ha podido aplicarse a la gravedad, un problema que continúa sin resolverse hasta el día de hoy dentro del modelo estándar. En el presente trabajo proponemos que la energía y el potencial de la carga autoinducida de una masa puntual a cierta distancia del campo generado por la masa o partícula, está dada por una función analítica, que conduce a la energía intrínseca de la masa cuando la distancia es nula, de la cual se derivan todas las fuerzas fundamentales de la naturaleza, incluida la gravedad, eliminándose las singularidades en las partículas fundamentales y los agujeros negros. Proponemos un modelo cosmológico de un universo cíclico basado en nuestras ecuaciones y la entropía de la antimateria como la antientropía de la materia y el orden en la naturaleza.
CULTURA ORGANIZACIONAL Y EL LOGRO DE LOS OBJETIVOS INSTITUCIONALES: CENTRO DE SALUD TIPO A, CASCOL
Authors: Soledispa Castro Ronald Andrés , Morejón Santistevan Maryury Elizabeth
Published: October 2023

Este estudio llevado a cabo en el Centro de Salud Tipo A, ubicado en la parroquia rural de Cascol, estableció de qué forma la cultura organizacional influye en el cumplimiento de los objetivos, además determinó sus fortalezas y debilidades. La metodología empleada fue con enfoque cualitativo, lo que permitió la recopilación y análisis de datos no numéricos. Además, se utilizó la técnica de encuesta dirigida a los médicos y el análisis FODA para evaluar la situación del Centro de Salud. Entre los resultados más notables, destaca que los objetivos institucionales no se logran de manera eficiente, en gran parte debido a la falta de comprensión de la cultura organizacional y a la insuficiente gestión de los factores internos y externos. Estos resultados subrayan la necesidad de abordar y fortalecer la cultura organizacional en el Centro de Salud como un paso esencial hacia la mejora de su rendimiento institucional.
EXÁMENES ESPECIALES EN LA ADQUISICIÓN DE BIENES Y SERVICIOS EN EL SECTOR PÚBLICO DEL ECUADOR
Authors: Calle García Aldrin Jefferson , Núñez Ugalde Juan Alfonso
Published: October 2023

Este estudio tuvo como finalidad analizar cuatro exámenes especiales sobre la adquisición de bienes y servicios en el sector público del Ecuador ejecutados por la Contraloría General del Estado, donde se pudo constatar diversas irregularidades en cuanto a la contratación pública, esto se pudo notar en el Gobierno Autónomo Descentralizado de la Provincia de Pichincha, Gobierno Autónomo Descentralizado Municipal de Rumiñahui, Empresa Pública Metropolitana de Hábitat y Vivienda y la Empresa Pública Municipal de Movilidad, Tránsito y Transporte de Cuenca, entidades tomadas como muestra de estudio. Además de identificar la aplicación de la normativa de contratación pública, sus resultados, conclusiones y recomendaciones. La metodología empleada en este estudio abordó la combinación de los métodos cualitativos y cualitativos, lo cual permitió obtener una comprensión precisa y detallada de la situación de cada una de las entidades respecto a la temática planteada.
METODOLOGÍA DE GESTIÓN TRIBUTARIA PARA MEJORA DE LAS OBLIGACIONES FISCALES DE LAS PERSONAS NATURALES NO OBLIGADAS A LLEVAR CONTABILIDAD DEL SECTOR COMERCIAL DEL CANTÓN JIPIJAPA
Authors: Quiñónez Cercado Mónica del Pilar , Lucio Vivar Alan Josué
Published: October 2023

Una gestión tributaria efectiva conlleva al cumplimiento de los deberes fiscales, es por ello que este estudio hizo su enfoque en las personas naturales no obligadas a llevar contabilidad del sector comercial del cantón Jipijapa-Ecuador. Su propósito central consistió en establecer la metodología de gestión tributaria para la mejora de los deberes fiscales de este tipo de contribuyentes, debido a las causas del incumplimiento de las mismas. Cabe señalar, que las personas naturales no obligadas a llevar contabilidad en relación a su labor financiera deben realizar la declaración de Impuesto al Valor Agregado (IVA), el Impuesto a la Renta (IR), entre otras obligaciones. La importancia de este estudio se centró en encuestar una muestra probabilística de 357 personas del sector comercial, por lo cual se determinó las causas del incumplimiento tributario, el 50% de las personas encuestadas afirman que las causas de sus incumplimientos son por la falta de conocimiento en temas tributarios. Por otra parte, en su metodología se emplearon varias técnicas como la revisión bibliográfica bajo un enfoque cualitativo y cuantitativo, el método exploratorio, descriptivo y el método estadístico lo que permitió tener una perspectiva clara de sus resultados y conclusiones.
A QUÍMICA E A SÍFILIS: UM PERCURSO HISTÓRICO
Authors: Silva Rodrigo , Filgueiras Carlos
Published: October 2023

CHEMISTRY AND SYPHILIS: A HISTORICAL JOURNEY. The relationship between syphilis and chemistry is remarkable due to its being the first example in western history of a comprehensive attempt by researchers from many countries and cultures to direct their efforts to tackle a serious health problem by means of chemical science. This effort started to take place at the time when the science of chemistry was already beginning to emerge, apart from alchemy. Thus, the fight against syphilis was closely linked to the emergence of iatrochemistry, or medicinal chemistry, which eventually gave rise, centuries later, to chemotherapy. Many of the early iatrochemical compounds in the XVIth century were of mineral origin, and often also synthetic. One of the pioneers of this trend was Paracelsus, who occupies a prominent place in the process. Along the centuries a number of different chemical approaches were attempted to cure syphilis until the advent of antibiotics in the mid-twentieth century, after so much time in which syphilis remained a physical and moral scourge of humankind, challenging all those that endeavoured to find its cure. In this article we recount many fascinating aspects of the application of chemistry in this quest, showing its development in the world as well as in Brazil, throughout its entire history.
Study of the Three-Dimensional Structure of Tryptophan Zipper Peptides through 1H NMR Chemical Shifts Calculations
Authors: de Albuquerque Ana Carolina , dos Santos Jr Fernando
Published: October 2023

Three-dimensional structures of proteins are intimately linked to their functions, therefore understanding their conformation in solution is essential. While nuclear magnetic resonance spectroscopy and X-ray crystallography are widely employed for protein structural determination, their limitations make the process challenging and expensive. Theoretical calculations of chemical shifts present a potential complement to experimental techniques, facilitating the study of protein structures. This investigation aims to assess the applicability of chemical shift calculations in analyzing three-dimensional structures of peptides, focusing on the tryptophan zipper 1 peptide as a model. Furthermore, a mutated variant of this peptide was proposed to evaluate the stability of its structural elements under sequence modifications. Through calculations, a potential structural alteration in the β-turn region of the mutant peptide compared to tryptophan zipper 1 was identified. This research demonstrates the potential of using computational approaches to complement experimental methods in studying protein structures and their functional implications.
The effect of fruit and berry supplements on the content of vitamin C in yoghurts
Authors: Kosikova Yu. , Korshik T. , Mastikhina A. , Limarov A.
Published: October 2023

In this article provides data research about changes of Vitamin C concentration in fruit and berry yogurts during the storage. Marked that correlation of the sort of fruit and berry supplement with the process of changing vitamin C content is negligible. The type of supplement has almost no effect on the safety of vitamin C during the storage of yoghurts and directly depends on the initial content of vitamin C in supplement. In this regard it was proposed to consider new non-traditional types of supllement with hight content of vitamin C in raw materials