bluuebunny/crossref_metadata_2025 huggingface dataset:
Composting Reduces the Vitality of H9N2 in Poultry Manure and EMCV in Pig Manure Allowing for an Environmentally Friendly Use of These Animal Wastes: A Preliminary Study
Authors: Jeong Kwang-Hwa , Lee Dong-Jun , Lee Dong-Hyun , Ravindran Balasubramani , Chang Soon Woong , Mupambwa Hupenyu Allan , Lee Myung-Gyu , Ahn Hee-Kwon
Published: June 2020

In our study, we monitored the inactivation of two important viruses that are critical in animal husbandry throughout the world. To evaluate the influence of the composting process on inactivation of avian influenza virus (H9N2) in poultry manure compost (PMC) and Encephalomyocarditis virus (EMCV) in pig (swine) manure compost (SMC), the H9N2 and EMCV were injected in dialysis cassettes and buried in two different manure compost piles of poultry and pig manure, respectively. The highest temperature achieved in the PMC and SMC piles during the test period were 75 °C and 73.5 °C, respectively. At the completion of the composting for 168 h, inactivation effect appeared to be more sensitive in H9N2 than EMCV. The vitality of H9N2 decreased by 6.25 ± 0.35 log10TCID50/mL to 0.0 log10TCID50/mL within 1 h of the composting. The vitality of EMCV decreased from 7.75 ± 0.35 log10TCID50/mL to 1.50 log10TCID50/mL within 24 h of starting the composting process. However, the activation of EMCV was not decreased (from 7.75 ± 0.35 to 7.50 ± 0.71 log10TCID50/mL) in the control treatment (not inserted in composts) after 168h, while the activation of H9N2 in dialysis cassettes was significantly decreased (from 6.25 ± 0.35 log10TCID50/mL to 2.00 ± 0.6 log10TCID50/mL). Our study demonstrated the effectiveness of the composting treatment in inactivating the viruses studied, which was not the case with air treatment.
Industrial Performance of Several Lachancea thermotolerans Strains for pH Control in White Wines from Warm Areas
Authors: Vaquero Cristian , Loira Iris , Bañuelos María Antonia , Heras José María , Cuerda Rafael , Morata Antonio
Published: June 2020

In the current scenario of climatic warming, the over-ripening of grapes increases the sugar content, producing flat and alcoholic wines with low acidity, high pH and low freshness. Additionally, a high pH makes wines more chemically and microbiologically unstable, requiring a higher sulphite content for preservation. Some strains of Lachancea thermotolerans can naturally lower the pH of wine by producing lactic acid from sugars; this pH reduction can reach 0.5 units. The industrial performance of four selected strains has been compared with that of two commercial strains and with that of Saccharomyces cerevisiae. The yeasts were assessed under variable oenological conditions, measuring lactic acid production and fermentative performance at two fermentation temperatures (17 and 27 °C), and in the presence or absence of sulphites (25 and 75 mg/L). Lactic acid production depends on yeast populations, with higher concentrations being reached when the microbial population is close to or above 7-log CFU/mL. A temperature effect on acidification can also be observed, being more intense at higher fermentation temperatures for most strains. Ethanol yield ranged from 7–11% vol., depending on the fermentation conditions (temperature and SO2) at day 12 of fermentation, compared with 12% for the S. cerevisiae control in micro-fermentations. The production of fermentative esters was higher at 27 °C compared with 17 °C, which favoured the production of higher alcohols. Volatile acidity was moderate under all fermentation conditions with values below 0.4 g/L.
Novel 2D and 3D Assays to Determine the Activity of Anti-Leishmanial Drugs
Authors: O’Keeffe Alec , Hale Christine , Cotton James A. , Yardley Vanessa , Gupta Kapish , Ananthanarayanan Abhishek , Murdan Sudaxshina , Croft Simon L.
Published: June 2020

The discovery of novel anti-leishmanial compounds remains essential as current treatments have known limitations and there are insufficient novel compounds in development. We have investigated three complex and physiologically relevant in vitro assays, including: (i) a media perfusion based cell culture model, (ii) two 3D cell culture models, and (iii) iPSC derived macrophages in place of primary macrophages or cell lines, to determine whether they offer improved approaches to anti-leishmanial drug discovery and development. Using a Leishmania major amastigote-macrophage assay the activities of standard drugs were investigated to show the effect of changing parameters in these assays. We determined that drug activity was reduced by media perfusion (EC50 values for amphotericin B shifted from 54 (51–57) nM in the static system to 70 (61–75) nM under media perfusion; EC50 values for miltefosine shifted from 12 (11–15) µM in the static system to 30 (26–34) µM under media perfusion) (mean and 95% confidence intervals), with corresponding reduced drug accumulation by macrophages. In the 3D cell culture model there was a significant difference in the EC50 values of amphotericin B but not miltefosine (EC50 values for amphotericin B were 34.9 (31.4–38.6) nM in the 2D and 52.3 (46.6–58.7) nM in 3D; EC50 values for miltefosine were 5.0 (4.9–5.2) µM in 2D and 5.9 (5.5–6.2) µM in 3D (mean and 95% confidence intervals). Finally, in experiments using iPSC derived macrophages infected with Leishmania, reported here for the first time, we observed a higher level of intracellular infection in iPSC derived macrophages compared to the other macrophage types for four different species of Leishmania studied. For L. major with an initial infection ratio of 0.5 parasites per host cell the percentage infection level of the macrophages after 72 h was 11.3% ± 1.5%, 46.0% ± 1.4%, 66.4% ± 3.5% and 75.1% ± 2.4% (average ± SD) for the four cells types, THP1 a human monocytic cell line, mouse bone marrow macrophages (MBMMs), human bone marrow macrophages (HBMMs) and iPSC derived macrophages respectively. Despite the higher infection levels, drug activity in iPSC derived macrophages was similar to that in other macrophage types, for example, amphotericin B EC50 values were 35.9 (33.4–38.5), 33.5 (31.5–36.5), 33.6 (30.5—not calculated (NC)) and 46.4 (45.8–47.2) nM in iPSC, MBMMs, HBMMs and THP1 cells respectively (mean and 95% confidence intervals). We conclude that increasing the complexity of cellular assays does impact upon anti-leishmanial drug activities but not sufficiently to replace the current model used in HTS/HCS assays in drug discovery programmes. The impact of media perfusion on drug activities and the use of iPSC macrophages do, however, deserve further investigation.
Structural Assessment of Fluorine, Chlorine, Bromine, Iodine, and Hydroxide Substitutions in Lead Arsenate Apatites (Mimetites)–Pb5(AsO4)3X
Authors: Sordyl Julia , Puzio Bartosz , Manecki Maciej , Borkiewicz Olaf , Topolska Justyna , Zelek-Pogudz Sylwia
Published: June 2020

Five lead-arsenate apatites (mimetites)-Pb5(AsO4)3X—where X denotes fluorine (F), chlorine (Cl), bromine (Br), iodine (I), and hydroxide (OH)—were synthesized via precipitation from aqueous solutions. The crystal structures were determined through Rietveld refinement of powder synchrotron X-ray data. All the compounds crystallized in the hexagonal class symmetry (space group P63/m). The Rietveld refinement indicated that mimetite-Cl, -Br, -I, and -OH had an anion deficiency at position X. Substitution of halogens in a mimetite structure brought about systematic changes in unit-cell parameters, interatomic distances, and metaprism twist angles φ, proportional to the substituted halogen’s ionic radius. Mimetite-OH did not follow the linear correlations determined within the series. Twist angle φ, a useful device for monitoring changes in apatite topology, ranged from 20.34° for mimetite-F to 11.42° for mimetite-I. The geometric method has been proposed for determining the diameter of hexagonal channels hosting halogens in apatites. A comparison of the results with halogenated pyromorphites showed similar systematic trends: the substitutions in mimetites have comparable effect on the interatomic distances as in their phosphorous analogues.
Concentration of Rare Earth Elements (Sc, Y, La, Ce, Nd, Sm) in Bauxite Residue (Red Mud) Obtained by Water and Alkali Leaching of Bauxite Sintering Dust
Authors: Chaikin Leonid , Shoppert Andrei , Valeev Dmitry , Loginova Irina , Napol’skikh Julia
Published: June 2020

One of the potential sources of rare-earth elements (REE) is the industrial waste known as red mud (bauxite residue), in which the majority of REE from the initial bauxite are concentrated via the Bayer process. Therefore, the studies of the subject, both in Russia and outside, focus almost exclusively on red mud processing. This article looks into the possibility of REE concentration into red mud by leaching an intermediate product of the bauxite sintering process at Russian alumina refineries, namely electrostatic precipitator (ESP) dust. The experimental works were performed by X-ray diffraction (XRD)and electron probe microanalysis (EPMA) of the sinter and sinter dust. The determination of major and rare-earth elements in the sinter from the rotary kilns and in the ESP dust before and after leaching was carried out by X-ray fluorescence (XRF) and plasma mass spectrometry (ICP-MS). The study showed that it is possible to obtain red mud that contains three times more REE than traditional waste red mud after two-stage leaching ESP dust in the water at 95 °C followed by leaching in an alkaline-aluminate liquor at 240 °C. The shrinking core model was used to study the kinetics of leaching of the original ESP dust and water-treated dust in alkaline-aluminate liquor. The study showed the change in the limiting stage of the alkaline leaching process after water treatment, with the activation energy growing from 24.98 to 33.19 kJ/mol.
Strain-Induced Fabric Transition of Chlorite and Implications for Seismic Anisotropy in Subduction Zones
Authors: Kim Dohyun , Jung Haemyeong , Lee Jungjin
Published: June 2020

Seismic anisotropy of S-wave, trench-parallel or trench-normal polarization direction of fast S-wave, has been observed in the fore-arc and back-arc regions of subduction zones. Lattice preferred orientation (LPO) of elastically anisotropic chlorite has been suggested as one of the major causes of seismic anisotropy in subduction zones. However, there are two different LPOs of chlorite reported based on the previous studies of natural chlorite peridotites, which can produce different expression of seismic anisotropy. The mechanism for causing the two different LPOs of chlorite is not known. Therefore, we conducted deformation experiments of chlorite peridotite under high pressure–temperature conditions (P = 0.5–2.5 GPa, T = 540–720 °C). We found that two different chlorite LPOs were developed depending on the magnitude of shear strain. The type-1 chlorite LPO is characterized by the [001] axes aligned subnormal to the shear plane, and the type-2 chlorite LPO is characterized by a girdle distribution of the [001] axes subnormal to the shear direction. The type-1 chlorite LPO developed under low shear strain (γ ≤ 3.1 ± 0.3), producing trench-parallel seismic anisotropy. The type-2 chlorite LPO developed under high shear strain (γ ≥ 5.1 ± 1.5), producing trench-normal seismic anisotropy. The anisotropy of S-wave velocity (AVs) of chlorite was very strong up to AVs = 48.7% so that anomalous seismic anisotropy in subduction zones can be influenced by the chlorite LPOs.
The Chemical State and Occupancy of Radiogenic Pb, and Crystallinity of RW-1 Monazite Revealed by XPS and TEM
Authors: Tang Xu , Li Qiu-Li , Zhang Bin , Wang Peng , Gu Li-Xin , Ling Xiao-Xiao , Fei Chen-Hui , Li Jin-Hua
Published: June 2020

Monazite ((Ce, La, Nd, Th)PO4) is one of the widely used minerals for U–Th–Pb dating in geochronology. To better understand the possible effects of radiogenic Pb on the in situ dating method, a natural monazite U–Th–Pb standard sample (RW-1) was chemically and structurally characterized down to atomic scales by using the combination of Raman spectrum (RM), X-ray photoelectron spectroscopy (XPS), and transmission electron microscopy (TEM). The experimental results revealed that radiogenic Pb exists as Pb2+ and substitutes for the Ce site in the monazite crystal lattice. Moreover, TEM imaging demonstrated that monazite is well crystalline revealed by an atomic structure in most areas except for a few tiny defects, which are likely attributed to alpha self-healing from an electronic energy loss of α particles. The characterization of the chemical state and occupancy of radiogenic Pb, and the distribution of Pb and Th in monazite at the nanoscale and atomic scale could provide insight for us to understand the mechanisms of the nanogeochronology.
Geochemical Significance of Clay Minerals and Elements in Paleogene Sandstones in the Center of the Northern Margin of the Qaidam Basin, China
Authors: Sun Guoqiang , Wang Meng , Guo Jiajia , Wang Yetong , Yang Yongheng
Published: June 2020

The average thickness of Paleogene sandstones reaches about 3000–4000 m at the northern margin of the Qaidam Basin. However, the provenance and sedimentary environment of these sandstones are uncertain; thus, more comprehensive research is needed. Integrated research is conducted on the provenance and weathering process based on petrographic characteristics, clay minerals, and geochemical compositions of sandstones in the center of the northern Qaidam Basin. The results of lithofacies analysis show that the Paleogene sandstones were mainly derived from an active continental magmatic arc, subduction accretion, or a fold-thrust belt. The average illite content in the Paleogene clay minerals is more than 50%, followed by chlorite and smectite, which reflect climatic and environmental characteristics that were arid to semi-arid, whereas the characteristics of carbon–oxygen isotopes reveal a mainly freshwater sedimentary environment. The corrected chemical index of alteration (CIAcorr) is between 56.3 and 75.7, with an average value of 66.5. These results indicate that the provenance of the Paleogene sandstones in the center of the northern Qaidam Basin mainly formed under cold and dry climatic conditions and experienced limited chemical weathering with a small amount that underwent intermediate chemical weathering under warm and humid conditions.
Lead Isotopes in Exploration for Basement-Hosted Structurally Controlled Unconformity-Related Uranium Deposits: Kiggavik Project (Nunavut, Canada)
Authors: Quirt David , Benedicto Antonio
Published: June 2020

Pb-isotopes have been proposed as pathfinders for sandstone-hosted unconformity-related U deposits, with isotope ratios providing information on mineralization timing and element remobilization and migration. Pb-isotopes proximal to mineralization display radiogenic signatures, often with ‘excess Pb’ suggestive of derivation from greater U concentrations than are currently present. The U deposits in the Kiggavik project area (west of Baker Lake, NU, Canada) are basement-hosted, contain several generations of pitchblende mineralization, display a strong structural control, and are located in fault-related fracture systems and foliation-parallel veinlets. Drill core samples were analysed by Inductively-Coupled Plasma-Mass Spectrometer (ICP-MS) for Pb isotopes following multi-acid total-digestion, reverse Aqua Regia partial-digestion, and weak-acid-leach attacks, to evaluate the utility of the respective dissolution methods in Pb-isotope pathfinder geochemistry. Partial-digestion results are similar to weak-acid-leach results, indicating that interpretation of Pb-isotope signatures can be carried out from partial-digestion data if weak-acid-leach data are unavailable. Application of this pathfinder method at Kiggavik shows that Pb-isotope ratios display systematic trends useful for exploration vectoring. Uranium-content-adjusted 206Pb/204Pb ratios and 206Pb/204Pb ‘excess-lead’ data highlight anomalous isotopic values. 207Pb/206Pb ratios display downhole trends complementary to location of mineralization. Three-dimensional (3D) distributions of Pb-isotope data at the Contact U prospect show systematic trends and form halos around the mineralization. Isotopic footprints are limited to <50 m from the mineralization outline, reflecting host-rock and structural control, but indicate areas with elevated potential for U mineralization and provide vectoring information within basement lithologies.
Terahertz Metamaterial with Multiple Resonances for Biosensing Application
Authors: Ou Huiliang , Lu Fangyuan , Xu Zefeng , Lin Yu-Sheng
Published: June 2020

A sickle-shaped metamaterial (SSM) based biochemical sensor with multiple resonances was investigated in the terahertz frequency range. The electromagnetic responses of SSM were found to be four resonances, namely dipolar, quadrupolar, octupolar and hexadecapolar plasmon resonances. They were generated from the interactions between SSM and perpendicularly incident terahertz waves. The sensing performances of SSM-based biochemical sensors were evaluated by changing ambient environments and analyte varieties. The highest values of sensitivity and figure of merit (FOM) for SSM covered with analyte thin-films were 471 GHz/RIU (refraction index unit) and 94 RIU−1, respectively. In order to further investigate the biosensing ability of the proposed SSM device, dielectric hemispheres and microfluidic chips were adopted to imitate dry and hydrous biological specimens, respectively. The results show that the sensing abilities of SSM-based biochemical sensors could be enhanced by increasing either the number of hemispheres or the channel width of the microfluidic chip. The highest sensitivity was 405 GHz/RIU for SSM integrated with microfluidic chips. Finally, three more realistic models were simulated to imitate real sensing situations, and the corresponding highest sensitivity was 502 GHz/RIU. The proposed SSM device paves the way to possible uses in biochemical sensing applications.
Al-Based Nano-Sized Composite Energetic Materials (Nano-CEMs): Preparation, Characterization, and Performance
Authors: Pang Weiqiang , Fan Xuezhong , Wang Ke , Chao Yimin , Xu Huixiang , Qin Zhao , Zhao Fengqi
Published: June 2020

As one of the new types of functional materials, nano-sized composite energetic materials (nano-CEMs) possess many advantages and broad application prospects in the research field of explosives and propellants. The recent progress in the preparation and performance characterization of Al-based nano-CEMs has been reviewed. The preparation methods and properties of Al-based nano-CEMs are emphatically analyzed. Special emphasis is focused on the improved performances of Al-based nano-CEMs, which are different from those of conventional micro-sized composite energetic materials (micro-CEMs), such as thermal decomposition and hazardous properties. The existing problems and challenges for the future work on Al-based nano-CEMs are discussed.
Probing the Mode of Antibacterial Action of Silver Nanoparticles Synthesized by Laser Ablation in Water: What Fluorescence and AFM Data Tell Us
Authors: Krce Lucija , Šprung Matilda , Rončević Tomislav , Maravić Ana , Čikeš Čulić Vedrana , Blažeka Damjan , Krstulović Nikša , Aviani Ivica
Published: June 2020

We aim to elucidate the mode of antibacterial action of the laser-synthesized silver colloid against Escherichia coli. Membrane integrity was studied by flow cytometry, while the strain viability of the treated culture was determined by plating. The spectrofluorometry was used to obtain the time development of the reactive oxygen species (ROS) inside the nanoparticle-treated bacterial cells. An integrated atomic force and bright-field/fluorescence microscopy system enabled the study of the cell morphology, Young modulus, viability, and integrity before and during the treatment. Upon lethal treatment, not all bacterial cells were shown to be permeabilized and have mostly kept their morphology with an indication of cell lysis. Young modulus of untreated cells was shown to be distinctly bimodal, with randomly distributed softer parts, while treated cells exhibited exponential softening of the stiffer parts in time. Silver nanoparticles and bacteria have shown a masking effect on the raw fluorescence signal through absorbance and scattering. The contribution of cellular ROS in the total fluorescence signal was resolved and it was proven that the ROS level inside the lethally treated cells is not significant. It was found that the laser-synthesized silver nanoparticles mode of antibacterial action includes reduction of the cell’s Young modulus in time and subsequently the cell leakage.
Reducing Nitrogen Dosage in Triticum durum Plants with Urea-Doped Nanofertilizers
Authors: Ramírez-Rodríguez Gloria B. , Miguel-Rojas Cristina , Montanha Gabriel S. , Carmona Francisco J. , Dal Sasso Gregorio , Sillero Josefina C. , Skov Pedersen Jan , Masciocchi Norberto , Guagliardi Antonietta , Pérez-de-Luque Alejandro , Delgado-López José M.
Published: June 2020

Nanotechnology is emerging as a very promising tool towards more efficient and sustainable practices in agriculture. In this work, we propose the use of non-toxic calcium phosphate nanoparticles doped with urea (U-ACP) for the fertilization of Triticum durum plants. U-ACP nanoparticles present very similar morphology, structure, and composition than the amorphous precursor of bone mineral, but contain a considerable amount of nitrogen as adsorbed urea (up to ca. 6 wt % urea). Tests on Triticum durum plants indicated that yields and quality of the crops treated with the nanoparticles at reduced nitrogen dosages (by 40%) were unaltered in comparison to positive control plants, which were given the minimum N dosages to obtain the highest values of yield and quality in fields. In addition, optical microscopy inspections showed that Alizarin Red S stained nanoparticles were able to penetrate through the epidermis of the roots or the stomata of the leaves. We observed that the uptake through the roots occurs much faster than through the leaves (1 h vs. 2 days, respectively). Our results highlight the potential of engineering nanoparticles to provide a considerable efficiency of nitrogen uptake by durum wheat and open the door to design more sustainable practices for the fertilization of wheat in fields.
Biodegradable and Antimicrobial PLA–OLA Blends Containing Chitosan-Mediated Silver Nanoparticles with Shape Memory Properties for Potential Medical Applications
Authors: Sonseca Agueda , Madani Salim , Muñoz-Bonilla Alexandra , Fernández-García Marta , Peponi Laura , Leonés Adrián , Rodríguez Gema , Echeverría Coro , López Daniel
Published: June 2020

To use shape memory materials based on poly (lactic acid) (PLA) for medical applications is essential to tune their transition temperature (Ttrans) near to the human body temperature. In this study, the combination of lactic acid oligomer (OLA), acting as a plasticizer, together with chitosan-mediated silver nanoparticles (AgCH-NPs) to create PLA matrices is studied to obtain functional shape memory polymers for potential medical applications. PLA/OLA nanocomposites containing different amounts of AgCH-NPs were obtained and profusely characterized relating their structure with their antimicrobial and shape memory performances. Nanocomposites exhibited shape memory responses at the temperature of interest (near physiological one), as well as excellent shape memory responses, shorter recovery times and higher recovery ratios (over 100%) when compared to neat materials. Moreover, antibacterial activity tests confirmed biocidal activity; therefore, these functional polymer nanocomposites with shape memory, degradability and biocidal activity show great potential for soft actuation applications in the medical field.
Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative Stress Followed by Processes of Autophagy and Programmed Cell Death
Authors: Balážová Ľudmila , Baláž Matej , Babula Petr
Published: June 2020

Nanomaterials, including zinc oxide nanoparticles (ZnO NPs), have a great application potential in many fields, such as medicine, the textile industry, electronics, and cosmetics. Their impact on the environment must be carefully investigated and specified due to their wide range of application. However, the amount of data on possible negative effects of ZnO NPs on plants at the cellular level are still insufficient. Thus, we focused on the effect of ZnO NPs on tobacco BY-2 cells, i.e., a widely accepted plant cell model. Adverse effects of ZnO NPs on both growth and biochemical parameters were observed. In addition, reactive oxygen and nitrogen species visualizations confirmed that ZnO NPs may induce oxidative stress. All these changes were associated with the lipid peroxidation and changes in the plasma membrane integrity, which together with endoplasmatic reticulum and mitochondrial dysfunction led to autophagy and programmed cell death. The present study demonstrates that the phytotoxic effect of ZnO NPs on the BY-2 cells is very complex and needs further investigation.
Interfacial Engineering of Pickering Emulsion Co-Stabilized by Zein Nanoparticles and Tween 20: Effects of the Particle Size on the Interfacial Concentration of Gallic Acid and the Oxidative Stability
Authors: Zhao Zijun , Wang Wenbo , Xiao Jie , Chen Yunjiao , Cao Yong
Published: June 2020

Lipid oxidation is still one of the major food-safety issues associated with the emulsion-based food systems. Engineering the interfacial region is an effective way to improve the oxidative stability of emulsion. Herein, a novel Pickering emulsion with strong oxidative stability was prepared by using zein nanoparticles and Tween 20 as stabilizers (ZPE). The modulation effects of the particle size on the distribution of gallic acid (GA) and the oxidative stability of ZPE were investigated. In the absence of GA, Pickering emulsions stabilized with different sizes of zein nanoparticles showed similar oxidative stability, and the physical barrier effect took the dominant role in retarding lipid oxidation. Moreover, in the presence of GA, ZPE stabilized by zein nanoparticles with the averaged particle size of 130 nm performed stronger oxidation than those stabilized by zein nanoparticles of 70 and 220 nm. Our study revealed that the interfacial concentration of GA (GAI) was tuned by zein nanoparticles due to the interaction between them, but the difference in the binding affinity between GA and zein nanoparticles was not the dominant factor regulating the (GAI). It was the interfacial content of zein nanoparticles (Γ), which was affected by the particle size, modulated the (GAI) and further dominated the oxidative stability of ZPEs. The present study suggested that the potential of thickening the interfacial layer to prevent lipid oxidation was limited, increasing the interfacial concentration of antioxidant by interfacial engineering offered a more efficient alternative.
Carbon Nanotube Film Electrodes with Acrylic Additives: Blocking Electrochemical Charge Transfer Reactions
Authors: Ansón-Casaos Alejandro , Sanahuja-Parejo Olga , Hernández-Ferrer Javier , Benito Ana M. , Maser Wolfgang K.
Published: June 2020

Carbon nanotubes (CNTs) processed into conductive films by liquid phase deposition technologies reveal increasing interest as electrode components in electrochemical device platforms for sensing and energy storage applications. In this work we show that the addition of acrylic latex to water-based CNT inks not only favors the fabrication of stable and robust flexible electrodes on plastic substrates but, moreover, sensitively enables the control of their electrical and electrochemical transport properties. Importantly, within a given concentration range, the acrylic additive in the films, being used as working electrodes, effectively blocks undesired faradaic transfer reactions across the electrode–electrolyte interface while maintaining their capacitance response as probed in a three-electrode electrochemical device configuration. Our results suggest a valuable strategy to enhance the chemical stability of CNT film electrodes and to suppress non-specific parasitic electrochemical reactions of relevance to electroanalytical and energy storage applications.
Non-Fullerene Small Molecule Electron-Transporting Materials for Efficient p-i-n Perovskite Solar Cells
Authors: Choi Da-Seul , Kwon Sung-Nam , Na Seok-In
Published: June 2020

PC61BM is commonly used in perovskite solar cells (PSC) as the electron transport material (ETM). However, PC61BM film has various disadvantages, such as its low coverage or the many pinholes that appear due to its aggregation behavior. These faults may lead to undesirable direct contact between the metal cathode and perovskite film, which could result in charge recombination at the perovskite/metal interface. In order to overcome this problem, three alternative non-fullerene electron materials were applied to inverted PSCs; they were evaluated on suitability as electron transport layers. The roles and effects of these non-fullerene ETMs on device performance were studied using photoluminescence (PL) measurements, field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), internal resistance in PSC measurements, and conductive atomic force microscopy (C-AFM). It was found that one of the tested materials, IT-4f, showed excellent electron extraction ability and was associated with reduced recombination. The PSC with IT-4f as the ETM produced better cell-performance; it had an average PCE of 11.21%, which makes it better than the ITIC and COi8DFIC-based devices. Finally, IT-4f was compared with PC61BM; it was found that the two materials have quite comparable efficiency and stability levels.
Binder Free and Flexible Asymmetric Supercapacitor Exploiting Mn3O4 and MoS2 Nanoflakes on Carbon Fibers
Authors: Rafique Amjid , Zubair Usman , Serrapede Mara , Fontana Marco , Bianco Stefano , Rivolo Paola , Pirri Candido F. , Lamberti Andrea
Published: June 2020

Emerging technologies, such as portable electronics, have had a huge impact on societal norms, such as access to real time information. To perform these tasks, portable electronic devices need more and more accessories for the processing and dispensation of the data, resulting in higher demand for energy and power. To overcome this problem, a low cost high-performing flexible fiber shaped asymmetric supercapacitor was fabricated, exploiting 3D-spinel manganese oxide Mn3O4 as cathode and 2D molybdenum disulfide MoS2 as anode. These asymmetric supercapacitors with stretched operating voltage window of 1.8 V exhibit high specific capacitance and energy density, good rate capability and cyclic stability after 3000 cycles, with a capacitance retention of more than 80%. This device has also shown an excellent bending stability at different bending conditions.
Are Supplements Safe? Effects of Gallic and Ferulic Acids on In Vitro Cell Models
Authors: Truzzi Francesca , Valerii Maria Chiara , Tibaldi Camilla , Zhang Yanxin , Abduazizova Veronika , Spisni Enzo , Dinelli Giovanni
Published: June 2020

Polyphenols display health-promoting properties linked to their biological activities. They are initially absorbed in the small intestine, then they are largely metabolized in the colon, whereupon they are able to exert systemic effects. The health-promoting properties of polyphenols have led to the development of food supplements, which are also largely consumed by healthy people, even if data on their safety are still yet lacking. In the present paper, the content of gallic acid and ferulic acid was analyzed in two supplements, and shown to be higher than the relative contents found in fruit and flour. To evaluate the effects of these phenolic compounds on epithelial intestinal tissue, gallic and ferulic acids were added to a new in vitro model of the intestinal wall at different concentrations. The effects on viability, proliferation and migration of these compounds were respectively tested on three different cell lines (Caco2, L929 and U937), as well as on a tridimensional intestinal model, composed of a mucosal layer and a submucosa with fibroblasts and monocytes. Results indicated that gallic and ferulic acids can exert toxic effects on in vitro cell models at high concentrations, suggesting that an excessive and uncontrolled consumption of polyphenols may induce negative effects on the intestinal wall.
Effects of Vegetarian Diets on Blood Pressure Lowering: A Systematic Review with Meta-Analysis and Trial Sequential Analysis
Authors: Lee Kai Wei , Loh Hong Chuan , Ching Siew Mooi , Devaraj Navin Kumar , Hoo Fan Kee
Published: June 2020

The beneficial effects of a vegetarian diet on blood pressure (BP) control have been reported in previous systematic reviews; however, so far, their relative effectiveness is not well established. Here, we performed a systematic review together with trial sequential analysis to determine the effect of a vegetarian diet on the reduction of blood pressure. We searched the randomized controlled trial (RCT) through Medline, PubMed and Cochrane Central Register. Fifteen eligible RCTs with 856 subjects were entered into the analysis. The pooled results demonstrated that vegetarian diet consumption significantly lowered the systolic blood pressure (weighted mean difference (WMD), −2.66 mmHg (95% confidence interval (CI) = −3.76, −1.55, p < 0.001) and diastolic BP was WMD, −1.69 95% CI = −2.97, −0.41, p < 0.001) as compared to an omnivorous diet. In subgroup analysis, a vegan diet demonstrated a greater reduction in systolic BP (WMD, −3.12 mmHg; 95% CI = −4.54, −1.70, p < 0.001) as compared with a lacto-ovo-vegetarian diet (WMD, −1.75 mmHg, 95% CI −5.38, 1.88, p = 0.05). The vegan diet has showed a similar trend in terms of diastolic blood pressure reduction (WMD, −1.92 mmHg (95% CI = −3.18, −0.66, p < 0.001) but those with a lacto-ovo-vegetarian diet showed no changes in diastolic BP reduction (WMD, 0.00, 95% CI = 0.00, 0.00), p = 0.432). In conclusion, vegetarian diets are associated with significant reductions in BP compared with omnivorous diets, suggesting that they may play a key role in the primary prevention and overall management of hypertension.
Textural Changes by Mastication and Proper Food Texture for Patients with Oropharyngeal Dysphagia
Authors: Matsuo Koichiro , Fujishima Ichiro
Published: June 2020

Bolus texture is a key factor for safe swallowing in patients with dysphagia since an improper texture may result in aspiration and/or pharyngeal residue. This article discusses swallowing bolus texture from two key aspects: the textural change of solid food by mastication and the current standardized definition of food texture in Japan. When swallowing a liquid bolus, the texture is mostly maintained from ingestion to swallow onset. For solid food, however, the food is crushed by chewing and mixed with saliva before swallowing; the texture of the ingested food is modified to an easily swallowable form at swallow onset by mastication. Understanding the mechanism of mastication and its assessment are therefore important in deciding the proper diet for dysphagic patients. As standardized criteria for classifying the texture of food and liquid are essential as well, this report also describes the Japanese Dysphagia Diet 2013 that is commonly used as the standardized index for dysphagic diets in Japan.
Cholecalciferol or Calcifediol in the Management of Vitamin D Deficiency
Authors: Sosa Henríquez Manuel , Gómez de Tejada Romero M. Jesús
Published: June 2020

Vitamin D deficiency is a global health problem due to its high prevalence and its negative consequences on musculoskeletal and extra-skeletal health. In our comparative review of the two exogenous vitamin D supplementation options most used in our care setting, we found that cholecalciferol has more scientific evidence with positive results than calcifediol in musculoskeletal diseases and that it is the form of vitamin D of choice in the most accepted and internationally recognized clinical guidelines on the management of osteoporosis. Cholecalciferol, unlike calcifediol, guarantees an exact dosage in IU (International Units) of vitamin D and has pharmacokinetic properties that allow either daily or even weekly, fortnightly, or monthly administration in its equivalent doses, which can facilitate adherence to treatment. Regardless of the pattern of administration, cholecalciferol may be more likely to achieve serum levels of 25(OH)D (25-hydroxy-vitamin D) of 30–50 ng/mL, an interval considered optimal for maximum benefit at the lowest risk. In summary, the form of vitamin D of choice for exogenous supplementation should be cholecalciferol, with calcifediol reserved for patients with liver failure or severe intestinal malabsorption syndromes.
Bovine Papillomavirus 1 Gets Out of the Flock: Detection in an Ovine Wart in Sicily
Authors: Savini Federica , Gallina Laura , Prosperi Alice , Puleio Roberto , Lavazza Antonio , Di Marco Patrizia , Tumino Serena , Moreno Ana , Lelli Davide , Guercio Annalisa , Scagliarini Alessandra
Published: June 2020

A proliferative cauliflower lesion was excised from the udder of a sheep. Histological investigation confirmed the macroscopic classification of the lesion as a papilloma, without any fibroblastic proliferation. PCR revealed the presence of bovine papillomavirus (BPV), which was further confirmed by the identification of a Deltapapillomavirus 4 by Next Generation Sequencing analysis. This was subsequently classified as bovine papillomavirus type 1. Negative staining electron microscopy (EM) analyses produced negative test results for papillomavirus particles. RNA in situ hybridization (ISH) confirmed the presence of BPV-1. The results further confirm the ability of BPVs belonging to the Deltapapillomavirus genus to infect distantly related species and to cause lesions that are different from sarcoids.
Parasite Cystatin: Immunomodulatory Molecule with Therapeutic Activity against Immune Mediated Disorders
Authors: Khatri Vishal , Chauhan Nikhil , Kalyanasundaram Ramaswamy
Published: June 2020

The use of parasites or their products for treating chronic inflammation associated diseases (CIADs) has generated significant attention recently. Findings from basic and clinical research have provided valuable information on strengthening the notion that parasites’ molecules can be developed as biotherapeutic agents. Completion of the genome, secreotome, and proteome of the parasites has provided an excellent platform for screening and identifying several host immunomodulatory molecules from the parasites and evaluate their therapeutic potential for CIADs. One of the widely studied host immunomodulatory molecules of the parasites is the cysteine protease inhibitor (cystatin), which is primarily secreted by the parasites to evade host immune responses. In this review, we have attempted to summarize the findings to date on the use of helminth parasite-derived cystatin as a therapeutic agent against CIADs. Although several studies suggest a role for alternatively activated macrophages, other regulatory cells, and immunosuppressive molecules, in this immunoregulatory activity of the parasite-derived cystatin, there is still no clear demonstration as to how cystatin induces its anti-inflammatory effect in suppressing CIADs.
Lack of Porcine circovirus 4 Genome Detection in Pig Samples from Italy and Spain
Authors: Franzo Giovanni , Ruiz Albert , Grassi Laura , Sibila Marina , Drigo Michele , Segalés Joaquim
Published: June 2020

The genus Circovirus includes several species and mostly causes asymptomatic infections. Porcine circovirus 2 (PCV-2) and, with increasing evidence, Porcine circovirus 3 (PCV-3), have been associated with different clinical conditions all over the world. In 2019, a new porcine circovirus (PCV-4) was identified from diseased animals in China. Because of the lessons learned from PCV-2 and PCV-3, it appears mandatory to investigate the actual distribution of this new virus and its potential association with clinical outcomes. To this purpose, an exploratory study to detect PCV-4 by molecular methods was performed in Italy and Spain by testing more than 300 samples of different types (serum and tissues), collected from both healthy and diseased pigs and wild boar as well. All samples, independently from the country, type, health status and host, tested PCV-4 negative. Therefore, no evidence of PCV-4 presence was found in Italy and Spain through this exploratory study. Considering the dense pig trade among European countries, its presence in the continent can similarly be considered unlikely. The reasons behind the restricted PCV-4 distribution compared to other porcine circoviruses will require further investigations. Careful surveillance might nevertheless be important since prompt recognition of PCV-4 would allow the implementation of effective countermeasures to prevent its spreading and potential economic losses.
“Stigma and HIV Are Like Brother and Sister!”: The Experience of African-Born Persons Living with HIV in the US
Authors: Cernasev Alina , Larson William L. , Peden-McAlpine Cynthia , Rockwood Todd , Ranelli Paul L. , Okoro Olihe , Schommer Jon C.
Published: June 2020

Minnesota has seen an increase in the number of immigrants from Africa, notably in the mid-1990s, making up around 2% of Minnesota’s total population. This population also faces many impediments that cause important difficulties not only for HIV prevention but also for treatment and care options. The objectives of this study were to capture the experiences of Persons Living with HIV (PLWH) in Minnesota (US) and to elicit their stories about their diagnosis news and what management strategies they use for coping with the stigma associated with the disease. Participants were recruited via fliers in pharmacies, clinics, and HIV service centers located in Minnesota. Recruitment continued until thematic saturation was obtained. Fourteen subjects participated in audio-recorded, semi-structured interviews that were transcribed verbatim into written text. The transcriptions were analyzed using Thematic Analysis. Three themes emerged from the data. Theme 1: Cruel News: “HIV-Oooooo! I wish I was dead”, Theme 2: This is My Secret! and Theme 3: “Stigma and HIV are brother and sister”. The results demonstrate that stigma is an ever-present problem in African-born PLWH living in the US. Participants perceived the stigma associated with HIV status to affect their lives and culture at individual, familial, and societal levels.
Assessing the Travel Health Knowledge of Australian Pharmacists
Authors: Heslop Ian M. , Speare Richard , Bellingan Michelle , Glass Beverley D.
Published: June 2020

Worldwide, the numbers of travellers are increasing, with pharmacists having the potential to play a significant role in the provision of pre-travel health services to a large number of these travellers. However, studies examining whether pharmacists have the travel health knowledge to provide these services are limited. This study thus aimed to explore the travel health knowledge of Australian pharmacists. Surveys assessing pharmacists’ knowledge of travel health were distributed through the Pharmaceutical Society of Australia and community pharmacies for self-completion. Overall, the travel health knowledge of participants was found to be good. However, although the majority of participants were aware of the common causes of morbidity and mortality in travel health, some slightly overestimated the prevalence of malaria and were less knowledgeable about the global distribution of some diseases. Most participants also demonstrated an ability to give appropriate advice on the management of traveller’s diarhoea, the selection of appropriate items for inclusion in travel first aid kits, vaccinations, and malarial chemoprophylaxis for travellers visiting endemic areas. This study highlights that Australian pharmacists have the knowledge to deliver travel health advice, with the potential to improve both access and outcomes for travellers.
Pharmacist—Pharmacy Technician Intraprofessional Collaboration and Workplace Integration: Implications for Educators
Authors: Jetha Maryam , Walji Ali , Gregory Paul , Abdulla Dalya , Austin Zubin
Published: June 2020

Globally, concerns have been expressed regarding the impact of regulation of pharmacy technicians. After more than a decade of experience with technician regulation in Ontario, Canada, uptake of the full scope of practice for technicians has been sporadic at best. The objective of this study was to examine barriers and facilitators to intraprofessional collaboration between pharmacists and pharmacy technicians for the purpose of identifying possible curricular or educational interventions to enhance workplace integration. A qualitative, interview-based study of 24 pharmacists, technicians, educators, pharmacy managers, and owners was undertaken using a semi-structured interview guide. Key findings of this research include: (i) Confirmation of suboptimal utilization of regulated technicians in practice; (ii) identification of crucial knowledge and skills gaps for both pharmacists and technicians; and (iii) proposals for undergraduate education and training, and continuing professional development learning opportunities to address these gaps. In order to achieve the promise and potential of regulation of pharmacy technicians, system-wide change management—beginning with education—will be required and will benefit from multiple stakeholder engagement and involvement.
The Power of a Controversial Issue; The Developmental Power of ‘Disruptive Moments’ for Both—Teachers and Students
Authors: ter Avest Ina
Published: June 2020

In Islamic primary education the subject of Islamic Religious Education (IRE) has a prominent place in the curriculum. In knowledge transfer and in behaving as a ‘good muslim’, aspects can be in conflict with generally accepted knowledge or customs in the Netherlands. The focus is on the power of a controversial issue (either or not wearing a head scarf) as a ‘disruptive moment’, stimulating pupils’ religious identity development. An example of recently developed teaching material illustrates the actual didactical approach regarding such a controversial issue. For a contextual understanding we start with a sketch of the Dutch educational system, and the position of Islamic education and its Islamic Religious Education (IRE) therein. In the second paragraph we describe the dialogical self theory (DST). DST’s relational understanding of a child’s development, including the developments regarding keeping a secret. In the third paragraph the case study is described, followed in the fourth paragraph by the introduction of Islamic teaching material and its way of approaching the controversial issue of the case study. We conclude our contribution with a discussion and recommendations for the development of teachers’ competencies in responding to controversial issues.