bluuebunny/crossref_metadata_2025 huggingface dataset:
The Evidence for Fertility Preservation in Pediatric Klinefelter Syndrome
Authors: Pook Celina J. , Cocca Alessandra , Grandone Anna , Al-Hussini Mohamed , Lam Wayne
Published: August 2021

Klinefelter syndrome (KS) is a common cause of non-obstructive azoospermia (NOA). Advances in fertility preservation (FP) techniques, such as the use of microdissection testicular sperm extraction (micro-TESE), have improved sperm retrieval rates (SRR) up to 40–50% in this population. Age has been suggested to have an impact on FP, postulating that sperm production may deteriorate over time due to germ cell loss. As such, sperm retrieval for patients with KS at a younger age has been proposed to further improve SRR; however, whether such practice pragmatically improves SRR is yet to be determined, and controversy remains with concerns over trauma caused by FP procedures on further impairment of testicular function. There has also been a debate on the ethics of performing FP procedures in the pediatric population. Optimizing FP for patients with KS invariably requires a holistic multidisciplinary approach. This review aimed to evaluate the latest evidence in performing FP in pediatric patients with KS, and discuss the controversy surrounding such practice. Hormonal changes in patients with KS during childhood and the use of hormonal manipulation to optimize SSR in this population have also been reviewed.
Machine Learning to Improve Orientation Estimation in Sports Situations Challenging for Inertial Sensor Use
Authors: van Dijk Marit P. , Kok Manon , Berger Monique A. M. , Hoozemans Marco J. M. , Veeger DirkJan H. E. J.
Published: August 2021

In sports, inertial measurement units are often used to measure the orientation of human body segments. A Madgwick (MW) filter can be used to obtain accurate inertial measurement unit (IMU) orientation estimates. This filter combines two different orientation estimates by applying a correction of the (1) gyroscope-based estimate in the direction of the (2) earth frame-based estimate. However, in sports situations that are characterized by relatively large linear accelerations and/or close magnetic sources, such as wheelchair sports, obtaining accurate IMU orientation estimates is challenging. In these situations, applying the MW filter in the regular way, i.e., with the same magnitude of correction at all time frames, may lead to estimation errors. Therefore, in this study, the MW filter was extended with machine learning to distinguish instances in which a small correction magnitude is beneficial from instances in which a large correction magnitude is beneficial, to eventually arrive at accurate body segment orientations in IMU-challenging sports situations. A machine learning algorithm was trained to make this distinction based on raw IMU data. Experiments on wheelchair sports were performed to assess the validity of the extended MW filter, and to compare the extended MW filter with the original MW filter based on comparisons with a motion capture-based reference system. Results indicate that the extended MW filter performs better than the original MW filter in assessing instantaneous trunk inclination (7.6 vs. 11.7° root-mean-squared error, RMSE), especially during the dynamic, IMU-challenging situations with moving athlete and wheelchair. Improvements of up to 45% RMSE were obtained for the extended MW filter compared with the original MW filter. To conclude, the machine learning-based extended MW filter has an acceptable accuracy and performs better than the original MW filter for the assessment of body segment orientation in IMU-challenging sports situations.
Whole Genome Sequencing Links Mycobacterium bovis From Cattle, Cheese and Humans in Baja California, Mexico
Authors: Ortiz Alejandro Perera , Perea Claudia , Davalos Enrique , Velázquez Estela Flores , González Karen Salazar , Camacho Erika Rosas , García Latorre Ethel Awilda , Lara Citlaltepetl Salinas , Salazar Raquel Muñiz , Bravo Doris M. , Stuber Tod P. , Thacker Tyler C. , Robbe-Austerman Suelee
Published: August 2021

Mycobacterium boviscauses tuberculosis (TB) in cattle, which in turn can transmit the pathogen to humans. Tuberculosis in dairy cattle is of particular concern where the consumption of raw milk and dairy products is customary. Baja California (BCA), Mexico, presents high prevalence of TB in both cattle and humans, making it important to investigate the molecular epidemiology of the disease in the region. A long-term study was undertaken to fully characterize the diversity ofM. bovisgenotypes circulating in dairy cattle, cheese and humans in BCA by whole-genome sequencing (WGS). During a 2-year period, 412 granulomatous tissue samples were collected from local abattoirs and 314 cheese samples were purchased from local stores and vendors in BCA and sent to the laboratory for mycobacterial culture, histology, direct PCR and WGS. For tissue samplesM. boviswas recovered from 86.8%, direct PCR detected 90% and histology confirmed 85.9% as mycobacteriosis-compatible. For cheese,M. boviswas recovered from 2.5% and direct PCR detected 6% of the samples. There was good agreement between diagnostic tests. Subsequently, a total of 345 whole-genome SNP sequences were obtained. Phylogenetic analysis grouped these isolates into 10 major clades. SNP analysis revealed putative transmission clusters where the pairwise SNP distance between isolates from different dairies was ≤3 SNP. Also, human and/or cheese isolates were within 8.45 (range 0–17) and 5.8 SNP (range 0–15), respectively, from cattle isolates. Finally, a comparison between the genotypes obtained in this study and those reported previously suggests that the genetic diversity ofM. bovisin BCA is well-characterized, and can be used to determine if BCA is the likely source ofM. bovisin humans and cattle in routine epidemiologic investigations and future studies. In conclusion, WGS provided evidence of ongoing local transmission ofM. bovisamong the dairies in this high-TB burden region of BCA, as well as show close relationships between isolates recovered from humans, cheese, and cattle. This confirms the need for a coordinated One Health approach in addressing the elimination of TB in animals and humans. Overall, the study contributes to the knowledge of the molecular epidemiology ofM. bovisin BCA, providing insight into the pathogen's dynamics in a high prevalence setting.
Immunohistochemical Characterisation of GLUT1, MMP3 and NRF2 in Osteosarcoma
Authors: Rutland Catrin S. , Cockcroft James M. , Lothion-Roy Jennifer , Harris Anna E. , Jeyapalan Jennie N. , Simpson Siobhan , Alibhai Aziza , Bailey Clara , Ballard-Reisch Alyssa C. , Rizvanov Albert A. , Dunning Mark D. , de Brot Simone , Mongan Nigel P.
Published: August 2021

Osteosarcoma (OSA) is an aggressive bone malignancy. Unlike many other malignancies, OSA outcomes have not improved in recent decades. One challenge to the development of better diagnostic and therapeutic methods for OSA has been the lack of well characterized experimental model systems. Spontaneous OSA in dogs provides a good model for the disease seen in people and also remains an important veterinary clinical challenge. We recently used RNA sequencing and qRT-PCR to provide a detailed molecular characterization of OSA relative to non-malignant bone in dogs. We identified differential mRNA expression of the solute carrier family 2 member 1 (SLC2A1/GLUT1), matrix metallopeptidase 3 (MMP3) and nuclear factor erythroid 2–related factor 2 (NFE2L2/NRF2) genes in canine OSA tissue in comparison to paired non-tumor tissue. Our present work characterizes protein expression of GLUT1, MMP3 and NRF2 using immunohistochemistry. As these proteins affect key processes such as Wnt activation, heme biosynthesis, glucose transport, understanding their expression and the enriched pathways and gene ontologies enables us to further understand the potential molecular pathways and mechanisms involved in OSA. This study further supports spontaneous OSA in dogs as a model system to inform the development of new methods to diagnose and treat OSA in both dogs and people.
Infectious Bovine Respiratory Diseases in Adult Cattle: An Extensive Necropsic and Etiological Study
Authors: Dorso Laëtitia , Rouault Maud , Barbotin Claire , Chartier Christophe , Assié Sébastien
Published: August 2021

In young cattle, bovine respiratory disease (BRD) is a major cause of death and Mannheimia haemolytica is a frequent pathogen. Knowledge of fatal BRD in adult cattle is more limited. We assessed the importance of infectious BRD as a cause of death in adult cattle and determined the associated pathogens. We analyzed data from 737 adult cattle necropsies at the Pathology Unit for Large Animals at Oniris, Nantes, France over a 6 year period (2013–2019). Each carcass was subjected to a complete necropsy. Lungs showing macroscopic lesions were classified into three categories: infectious primary pulmonary (IPP) lesions, thromboembolic pneumonia (TEP) and others (aspiration pneumonia, verminous pneumonia, and local extension of an extra-pulmonary inflammatory process). Half of the lungs with IPP macroscopic lesions were sampled for histology and submitted for polymerase chain reaction. BRD was the second leading cause of death (15.7%) after digestive diseases (32.2%). A strong predominance of IPP lesions (42.3%) and TEP lesions (39.6%) was also demonstrated. In IPP macroscopic lesions, fibrinous, hemorrhagic and/or hecrotic (FHN) bronchopneumonia accounted for 77.6% of macroscopic lesions. Mannheimia haemolytica was significantly associated with FHN bronchopneumonia macroscopic lesions. This study suggests that Mannheimia haemolytica should be included in the differential diagnosis of BRD in adult cattle.
GDAP1 Involvement in Mitochondrial Function and Oxidative Stress, Investigated in a Charcot-Marie-Tooth Model of hiPSCs-Derived Motor Neurons
Authors: Miressi Federica , Benslimane Nesrine , Favreau Frédéric , Rassat Marion , Richard Laurence , Bourthoumieu Sylvie , Laroche Cécile , Magy Laurent , Magdelaine Corinne , Sturtz Franck , Lia Anne-Sophie , Faye Pierre-Antoine
Published: August 2021

Mutations in the ganglioside-induced differentiation associated protein 1 (GDAP1) gene have been associated with demyelinating and axonal forms of Charcot-Marie-Tooth (CMT) disease, the most frequent hereditary peripheral neuropathy in humans. Previous studies reported the prevalent GDAP1 expression in neural tissues and cells, from animal models. Here, we described the first GDAP1 functional study on human induced-pluripotent stem cells (hiPSCs)-derived motor neurons, obtained from normal subjects and from a CMT2H patient, carrying the GDAP1 homozygous c.581C>G (p.Ser194*) mutation. At mRNA level, we observed that, in normal subjects, GDAP1 is mainly expressed in motor neurons, while it is drastically reduced in the patient’s cells containing a premature termination codon (PTC), probably degraded by the nonsense-mediated mRNA decay (NMD) system. Morphological and functional investigations revealed in the CMT patient’s motor neurons a decrease of cell viability associated to lipid dysfunction and oxidative stress development. Mitochondrion is a key organelle in oxidative stress generation, but it is also mainly involved in energetic metabolism. Thus, in the CMT patient’s motor neurons, mitochondrial cristae defects were observed, even if no deficit in ATP production emerged. This cellular model of hiPSCs-derived motor neurons underlines the role of mitochondrion and oxidative stress in CMT disease and paves the way for new treatment evaluation.
Evaluation of β-Catenin Inhibition of Axitinib and Nitazoxanide in Human Monocyte-Derived Dendritic Cells
Authors: Azeem Waqas , Bakke Ragnhild Maukon , Gabriel Benjamin , Appel Silke , Øyan Anne Margrete , Kalland Karl-Henning
Published: August 2021

Modulation of β-catenin signaling has attractive therapeutic potential in cancer immunotherapy. Several studies have found that β-catenin can mediate immune evasion in cancer and promote anti-inflammatory features of antigen-presenting dendritic cells. Many small molecular compounds that inhibit Wnt/β-catenin signaling are currently in clinical development, but none have entered routine clinical use. New inhibitors of β-catenin signaling are consequently desirable. Here, we have tested, in monocyte-derived dendritic cells, the effects of two small molecular compounds, axitinib and nitazoxanide, that previously have been discovered to inhibit β-catenin signaling in colon cancer cells. Immature and lipopolysaccharide-matured dendritic cells prepared from healthy blood donor buffy coats were stimulated with 6-bromoindirubin-3′-oxime (6-BIO) to boost basal β-catenin activity, and the effects of axitinib and nitazoxanide were compared with the commercial β-catenin inhibitor ICG-001. Assays, including genome-wide RNA-sequencing, indicated that neither axitinib nor nitazoxanide demonstrated considerable β-catenin inhibition. Both compounds were found to be less toxic to monocyte-derived dendritic cells than either 6-BIO or ICG-001. Axitinib stimulated several aspects of dendritic cell function, such as IL12-p70 secretion, and counteracted IL-10 secretion, according to the present study. However, neither axitinib nor nitazoxanide were found to be efficient β-catenin inhibitors in monocyte-derived dendritic cells.
Rapid Detection of Fatty Acids in Edible Oils Using Vis-NIR Reflectance Spectroscopy with Multivariate Methods
Authors: Su Ning , Pan Fangfang , Wang Liusan , Weng Shizhuang
Published: August 2021

The composition and content of fatty acids are critical indicators to identify the quality of edible oils. This study was undertaken to establish a rapid determination method for quality detection of edible oils based on quantitative analysis of palmitic acid, stearic acid, arachidic acid, and behenic acid. Seven kinds of oils were measured to obtain Vis-NIR spectra. Multivariate methods combined with pretreatment methods were adopted to establish quantitative analysis models for the four fatty acids. The model of support vector machine (SVM) with standard normal variate (SNV) pretreatment showed the best predictive performance for the four fatty acids. For the palmitic acid, the determination coefficient of prediction (RP2) was 0.9504 and the root mean square error of prediction (RMSEP) was 0.8181. For the stearic acid, RP2 and RMSEP were 0.9636 and 0.2965. In the prediction of arachidic acid, RP2 and RMSEP were 0.9576 and 0.0577. In the prediction of behenic acid, the RP2 and RMSEP were 0.9521 and 0.1486. Furthermore, the effective wavelengths selected by successive projections algorithm (SPA) were useful for establishing simplified prediction models. The results demonstrate that Vis-NIR spectroscopy combined with multivariate methods can provide a rapid and accurate approach for fatty acids detection of edible oils.
Coenzyme a Biochemistry: From Neurodevelopment to Neurodegeneration
Authors: Mignani Luca , Gnutti Barbara , Zizioli Daniela , Finazzi Dario
Published: August 2021

Coenzyme A (CoA) is an essential cofactor in all living organisms. It is involved in a large number of biochemical processes functioning either as an activator of molecules with carbonyl groups or as a carrier of acyl moieties. Together with its thioester derivatives, it plays a central role in cell metabolism, post-translational modification, and gene expression. Furthermore, recent studies revealed a role for CoA in the redox regulation by the S-thiolation of cysteine residues in cellular proteins. The intracellular concentration and distribution in different cellular compartments of CoA and its derivatives are controlled by several extracellular stimuli such as nutrients, hormones, metabolites, and cellular stresses. Perturbations of the biosynthesis and homeostasis of CoA and/or acyl-CoA are connected with several pathological conditions, including cancer, myopathies, and cardiomyopathies. In the most recent years, defects in genes involved in CoA production and distribution have been found in patients affected by rare forms of neurodegenerative and neurodevelopmental disorders. In this review, we will summarize the most relevant aspects of CoA cellular metabolism, their role in the pathogenesis of selected neurodevelopmental and neurodegenerative disorders, and recent advancements in the search for therapeutic approaches for such diseases.
The Most Common VHL Point Mutation R167Q in Hereditary VHL Disease Interferes with Cell Plasticity Regulation
Authors: Buart Stéphanie , Terry Stéphane , Diop M’boyba Khadija , Dessen Philippe , Couvé Sophie , Abdou Abdérémane , Adam Julien , Thiery Jérôme , Savagner Pierre , Chouaib Salem
Published: August 2021

Von Hippel–Lindau disease (VHL) is a rare hereditary syndrome due to mutations of the VHL tumor suppressor gene. Patients harboring the R167Q mutation of the VHL gene have a high risk of developing ccRCCs. We asked whether the R167Q mutation with critical aspects of pseudo-hypoxia interferes with tumor plasticity. For this purpose, we used wild-type VHL (WT-VHL) and VHL-R167Q reconstituted cells. We showed that WT-VHL and VHL-R167Q expression had a similar effect on cell morphology and colony formation. However, cells transfected with VHL-R167Q display an intermediate, HIF2-dependent, epithelial–mesenchymal phenotype. Using RNA sequencing, we showed that this mutation upregulates the expression of genes involved in the hypoxia pathway, indicating that such mutation is conferring an enhanced pseudo-hypoxic state. Importantly, this hypoxic state correlates with the induction of genes belonging to epithelial–mesenchymal transition (EMT) and stemness pathways, as revealed by GSEA TCGA analysis. Moreover, among these deregulated genes, we identified nine genes specifically associated with a poor patient survival in the TCGA KIRC dataset. Together, these observations support the hypothesis that a discrete VHL point mutation interferes with tumor plasticity and may impact cell behavior by exacerbating phenotypic switching. A better understanding of the role of this mutation might guide the search for more effective treatments to combat ccRCCs.
Gold Nanoparticles-Induced Modifications in Cell Wall Composition in Barley Roots
Authors: Milewska-Hendel Anna , Sala Katarzyna , Gepfert Weronika , Kurczyńska Ewa
Published: August 2021

The increased use of nanoparticles (NP) in different industries inevitably results in their release into the environment. In such conditions, plants come into direct contact with NP. Knowledge about the uptake of NP by plants and their effect on different developmental processes is still insufficient. Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP). The analyses were performed using the immunohistological method and fluorescence microscopy. The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
Comprehensive Omics Analysis of a Novel Small-Molecule Inhibitor of Chemoresistant Oncogenic Signatures in Colorectal Cancer Cell with Antitumor Effects
Authors: Huang Tse-Hung , Mokgautsi Ntlotlang , Huang Yan-Jiun , Wu Alexander T. H. , Huang Hsu-Shan
Published: August 2021

Tumor recurrence from cancer stem cells (CSCs) and metastasis often occur post-treatment in colorectal cancer (CRC), leading to chemoresistance and resistance to targeted therapy. MYC is a transcription factor in the nuclei that modulates cell growth and development, and regulates immune response in an antitumor direction by mediating programmed death ligand 1 (PD-L1) and promoting CRC tumor recurrence after adjuvant chemotherapy. However, the molecular mechanism through which c-MYC maintains stemness and confers treatment resistance still remains elusive in CRC. In addition, recent reports demonstrated that CRC solid colon tumors expresses C-X-C motif chemokine ligand 8 (CXCL8). Expression of CXCL8 in CRC was reported to activate the expression of PD-L1 immune checkpoint through c-MYC, this ultimately induces chemoresistance in CRC. Accumulating studies have also demonstrated increased expression of CXCL8, matrix metalloproteinase 7 (MMP7), tissue inhibitor of metalloproteinase 1 (TIMP1), and epithelial-to-mesenchymal transition (EMT) components, in CRC tumors suggesting their potential collaboration to promote EMT and CSCs. TIMP1 is MMP-independent and regulates cell development and apoptosis in various cancer cell types, including CRC. Recent studies showed that TIMP1 cleaves CXCL8 on its chemoattractant, thereby influencing its mechanistic response to therapy. This therefore suggests crosstalk among the c-MYC/CXCL8/TIMP1 oncogenic signatures. In this study, we explored computer simulations through bioinformatics to identify and validate that the MYC/CXCL8/TIMP1 oncogenic signatures are overexpressed in CRC, Moreover, our docking results exhibited putative binding affinities of the above-mentioned oncogenes, with our novel small molecule, RV59, Finally, we demonstrated the anticancer activities of RV59 against NCI human CRC cancer cell lines both as single-dose and dose-dependent treatments, and also demonstrated the MYC/CXCL8/TIMP1 signaling pathway as a potential RV59 drug target.
Nephrotoxic Metal Mixtures and Preadolescent Kidney Function
Authors: Levin-Schwartz Yuri , Politis Maria , Gennings Chris , Tamayo-Ortiz Marcela , Flores Daniel , Amarasiriwardena Chitra , Pantic Ivan , Tolentino Mari , Estrada-Gutierrez Guadalupe , Lamadrid-Figueroa Hector , Tellez-Rojo Martha , Baccarelli Andrea , Wright Robert , Sanders Alison
Published: August 2021

Exposure to metals including lead (Pb), cadmium (Cd), and arsenic (As), may impair kidney function as individual toxicants or in mixtures. However, no single medium is ideal to study multiple metals simultaneously. We hypothesized that multi-media biomarkers (MMBs), integrated indices combining information across biomarkers, are informative of adverse kidney function. Levels of Pb, Cd, and As were quantified in blood and urine in 4–6-year-old Mexican children (n = 300) in the PROGRESS longitudinal cohort study. We estimated the mixture effects of these metals, using weighted quantile sum regression (WQS) applied to urine biomarkers (Umix), blood biomarkers (Bmix), and MMBs, on the cystatin C-based estimated glomerular filtration rate (eGFR) and serum cystatin C assessed at 8–10 years of age, adjusted for covariates. Quartile increases in Umix and the MMB mixture were associated with 2.5% (95%CI: 0.1, 5.0) and 3.0% (95%CI: 0.2, 5.7) increased eGFR and −2.6% (95% CI: −5.1%, −0.1%) and −3.3% (95% CI: −6.5%, −0.1%) decreased cystatin C, respectively. Weights indicate that the strongest contributors to the associations with eGFR and serum cystatin C were Cd and Pb, respectively. MMBs detected mixture effects distinct from associations with individual metals or media-type, highlighting the benefits of incorporating information from multiple exposure media in mixtures analyses.
Dental Caries and Oral Health in Children—Special Issue
Authors: Tadakamadla Santosh Kumar , Tartaglia Gianluca Martino
Published: August 2021

Oral diseases still pose a significant health burden affecting over 3 [...]
Dietary Sugar Intake and Its Association with Obesity in Children and Adolescents
Authors: Magriplis Emmanuella , Michas George , Petridi Evgenia , Chrousos George P. , Roma Eleftheria , Benetou Vassiliki , Cholopoulos Nikos , Micha Renata , Panagiotakos Demosthenes , Zampelas Antonis
Published: August 2021

Sugar intake has been associated with increased prevalence of childhood overweight/obesity; however, results remain controversial. The aim of this study was to examine the probability of overweight/obesity with higher sugar intakes, accounting for other dietary intakes. Data from 1165 children and adolescents aged ≥2–18 years (66.8% males) enrolled in the Hellenic National Nutrition and Health Survey (HNNHS) were used; specifically, 781 children aged 2–11 years and 384 adolescents 12–18 years. Total and added sugar intake were assessed using two 24 h recalls (24 hR). Foods were categorized into specific food groups to evaluate the main foods contributing to intakes. A significant proportion of children (18.7%) and adolescents (24.5%) exceeded the recommended cut-off of 10% of total energy intake from added sugars. Sweets (29.8%) and processed/refined grains and cereals (19.1%) were the main sources of added sugars in both age groups, while in adolescents, the third main contributor was sugar-sweetened beverages (20.6%). Being overweight or obese was 2.57 (p = 0.002) and 1.77 (p = 0.047) times more likely for intakes ≥10% of total energy from added sugars compared to less <10%, when accounting for food groups and macronutrient intakes, respectively. The predicted probability of becoming obese was also significant with higher total and added-sugar consumption. We conclude that high consumption of added sugars increased the probability for overweight/obesity among youth, irrespectively of other dietary or macronutrient intakes.
Utility of Laparoscopic Approach of Orchiopexy for Palpable Cryptorchidism: A Systematic Review and Meta-Analysis
Authors: Anand Sachit , Krishnan Nellai , Pogorelić Zenon
Published: August 2021

Background: Traditional open orchiopexy is still a standard of treatment for palpable undescended testicles. Recently several authors reported successful results using a laparoscopic approach in the treatment of palpable cryptorchidism. The present systematic review and meta-analysis investigated the utility of laparoscopic orchiopexy for palpable cryptorchidism. Methods: Scientific databases (PubMed, Scopus, Web of Science, and EMBASE) were systematically searched for relevant articles using the following terms: (palpable cryptorchidism or palpable undescended testes) AND (laparoscopic orchiopexy or laparoscopic orchiopexy). The inclusion criteria were all children with unilateral or bilateral palpable undescended testes who underwent laparoscopic orchiopexy (LO) compared to children who underwent conventional open orchiopexy (CO). The main outcomes were the proportion of children requiring redo-orchiopexy and the incidence of postoperative complications. Secondary outcomes were duration and the cost of surgery. Results: The final meta-analysis included five studies involving 705 children; LO, n = 369 (52.3%) and CO, n = 336 (47.7%). The majority of the included patients had unilateral palpable cryptorchidism. No significant differences were found in regard to average age at the time of surgery and follow-up periods between the investigated groups. No statistically significant differences were found in regard to redo-orchiopexy rates (RR = 0.22, 95% CI 0.03–1.88, p = 0.17), early complications (RR = 0.66, 95% CI 0.21–2.08, p = 0.48) and incidence of testicular atrophy (RR = 0.36, 95% CI 0.03–3.88, p = 0.40). No significant differences in the operative duration were observed among the groups. Laparoscopy was associated with higher costs in most of the studies. Conclusion: LO is safe and effective in children with palpable cryptorchidism. The rates of redo-orchiopexy as well as an incidence of early complications and testicular atrophy rates are comparable to CO.
The Utilization of an Opioid-Free Anesthetic for Pediatric Circumcision in an Ambulatory Surgery Center
Authors: Gilbertson Laura E. , Patel Chhaya , De Shuvro , Lo Wendy , Garcia-Roig Michael , Austin Thomas M.
Published: August 2021

Circumcision is one of the most common urologic procedures performed at pediatric ambulatory centers. Emerging data on the short- and long-term effects of perioperative opioid administration has highlighted the importance of an opioid-free anesthetic regimen. We sought to evaluate the effectiveness of an opioid-free anesthetic in pediatric circumcision and its correlation with ambulatory surgery center efficiency. Patients, 3 years of age and younger, who underwent circumcision or circumcision revision by two surgeons pre and post introduction of an opioid-free anesthetic fast-track regimen at an outpatient surgical center were included. There were 100 patients included in this analysis, with 50 patients in each cohort. On univariate analysis, fast-tracking was associated with a decrease in median combined in-room and post-anesthesia care unit times (102.5 vs. 129.0 min, p-value < 0.001). This difference continued after multivariable analysis with an adjusted median combined in-room and post-anesthesia care unit time difference of −15.6 min (95% CI −34.2 to −12.7 min, p-value 0.018). In addition, the fast-track cohort received less intraoperative morphine equivalents without an increase in post-operative analgesic administration or change in postoperative questionnaire score. This demonstrates that opioid-free anesthesia may be used effectively in pediatric circumcision while also allowing for significant time savings for surgical centers.
Desulfurization Ability of Blast Furnace Slag Containing High Al2O3 at 1773 K
Authors: Guo Yongchun , Shen Fengman , Zheng Haiyan , Wang Shuo , Jiang Xin , Gao Qiangjian
Published: August 2021

With the consumption of high-quality iron ore resources, the grade of iron ore raw used in the iron making process has gradually decreased. The high aluminum raw materials has led to an increase in the Al2O3 content in the blast furnace slag, which has affected the normal operation of the blast furnace. The activity of the components involved in the desulfurization reaction is an important factor affecting the desulfurization process. In this paper, the effects of B(w(CaO)/w(SiO2)) and w(MgO)/w(Al2O3) on the desulfurization ability of a CaO-SiO2-MgO-Al2O3 quaternary blast furnace slag system were studied by using a double-layer graphite crucible to simulate the process of molten iron dropping through the slag at 1773 K. The desulfurization reaction mechanism of high alumina blast furnace slag is explained from the aspects of slag structure and component activity, which provides a theoretical basis for the reasonable selection of a slag making system under the condition of a high alumina content. The effects of different B and w(MgO)/w(Al2O3) on the slag structure 2343 analyzed by Fourier transform infrared spectroscopy (FT-IR). The results show that when w(MgO)/w(Al2O3) = 0.50 and w(Al2O3) = 20%, the desulfurization ability and MgO activity of slag increased with the increase of B; when w(Al2O3) = 20% and B = 1.30, the desulfurization ability and MgO activity of the slag increased with the increase of w(MgO)/w(Al2O3). The FT-IR analysis showed that with the increase of B or w(MgO)/w(Al2O3), the dissociated free oxygen ions (O2−) in the slag increased, and the dissociated free oxygen ions (O2−) interacted with the bridging oxygen (O0) of silicate, which made the complex Si-O structure in the slag gradually depolymerize and increase the desulfurization ability.
Cariogenic Biofilms: Development, Properties, and Biomimetic Preventive Agents
Authors: Meyer Frederic , Enax Joachim , Epple Matthias , Amaechi Bennett T. , Simader Barbara
Published: August 2021

Oral biofilms will build up within minutes after cleaning of the dental hard tissues. While the application of remineralizing agents is a well-known approach to prevent dental caries, modern oral care products offer also additional active agents to maintain oral health. Human saliva contains many different organic and inorganic compounds that help to buffer organic acids produced by cariogenic microorganisms. However, most oral care products only contain remineralizing agents. To improve the benefit of those products, further active ingredients are needed. Books, review articles, and original research papers were included in this narrative review. Putting all these data together, we give an overview of oral biofilms and active compounds used in modern oral care products to interact with them. The special focus is on inorganic compounds and their interaction with oral biofilms. While organic compounds have several limitations (e.g., cell toxicity), inorganic compounds based on calcium and/or phosphate (e.g., sodium bicarbonate, hydroxyapatite, calcium carbonate) offer several advantages when used in oral care products. Calcium release can inhibit demineralization, and the release of hydroxide and phosphate ions might help in the buffering of acids. Therefore, the focus of this review is to summarize the scientific background of further active ingredients that can be used for oral care formulations.
Sub-/Super-SCI Influencing Factors Analysis of VSC-HVDC and PMSG-Wind Farm System by Impedance Bode Criterion
Authors: Zhang Yibo , Tian Xu , Wang Cong , Cheng Hong
Published: August 2021

A sub-/super-synchronous interaction (sub-/super-SCI) can occur between a voltage source converter-based high-voltage direct current (VSC-HVDC) and the permanent magnet synchronous generator (PMSG)-based wind farms with long AC transmission lines. However, the influencing factors have not been properly analyzed. In this paper, these are deconstructed and mathematically analyzed from detailed small-signal impedance equations in the dq-frame and the corresponding Bode stability criterion. Distinguishing conclusions from existing papers are obtained by studying the controllers’ bandwidths instead of their coefficients. The impacts of AC line impedance on system stability are also investigated. From the analysis of their compositions in impedance structure, the VSC-HVDC bandwidths and the wind farm phase-locked loop (PLL) bandwidth and power ratio, and the AC line impedance have various influences on the system stability. Meanwhile, the wind farm outer DC voltage and inner current control bandwidths have little impact on system stability. The results of these studies show that the magnitude in the axes q-axes impedance interaction is the essential factor for system instability. Our studies also show system stability is more sensitive to the HVDC bandwidths than the wind converter PLL bandwidth. The simulation results verify our theory conclusions.
Analysis of Natural and Power Plant CO2 Emissions in the Mount Amiata (Italy) Volcanic–Geothermal Area Reveals Sustainable Electricity Production at Zero Emissions
Authors: Sbrana Alessandro , Lenzi Alessandro , Paci Marco , Gambini Roberto , Sbrana Michele , Ciani Valentina , Marianelli Paola
Published: August 2021

Geothermal energy is a key renewable energy for Italy, with an annual electric production of 6.18 TWh. The future of geothermal energy is concerned with clarity over the CO2 emissions from power plants and geological contexts where CO2 is produced naturally. The Mt. Amiata volcanic–geothermal area (AVGA) is a formidable natural laboratory for investigating the relative roles of natural degassing of CO2 and CO2 emissions from geothermal power plants (GPPs). This research is based on measuring the soil gas flux in the AVGA and comparing the diffuse volcanic soil gas emissions with the emissions from geothermal fields in operation. The natural flux of soil gas is high, independently from the occurrence of GPPs in the area, and the budget for natural diffuse gas flux is high with respect to power plant gas emissions. Furthermore, the CO2 emitted from power plants seems to reduce the amount of natural emissions because of the gas flow operated by power plants. During the GPPs’ life cycle, CO2 emissions in the atmosphere are reduced further because of the reinjection of gas-free aqueous fluids in geothermal reservoirs. Therefore, the currently operating GPPs in the AVGA produce energy at a zero-emission level.
Fabrication of a Sensitive and Stable NiO Uric Acid Biosensor Using Ag Nanowires and Reduced Graphene Oxide
Authors: Chou Jung-Chuan , Lai Tsu-Yang , Nien Yu-Hsun , Lai Chih-Hsien , Kuo Po-Yu , Lin Si-Hong , Huang Yu-Hao , Lee Kun-Tse
Published: August 2021

How to detect uric acid is an important issue. For the purpose of preparing a potentiometric uric acid biosensor, this research used nickel oxide (NiO) as the sensing film to deposit it onto the substrate by radio frequency sputtering, then modified it with reduced graphene oxide (rGO) and silver (Ag) nanowires. Reduced graphene oxide (rGO) not only has excellent electrical conductivity, but also can make the surface of the film have a larger surface area, while AgNWs have also been proven to improve catalytic activity; hence, these two materials were chosen as sensor modifiers. Finally, the stability and the various characteristics of the uric acid biosensor were investigated using a voltage–time (V–T) system. The results showed that the AgNW–uricase/rGO/NiO uric acid biosensor has average sensitivity with 4.66 mV/(mg/L). In addition, the sensor has good stability.
Is Secure Communication in the R2I (Robot-to-Infrastructure) Model Possible? Identification of Threats
Authors: Krzykowska-Piotrowska Karolina , Dudek Ewa , Siergiejczyk Mirosław , Rosiński Adam , Wawrzyński Wojciech
Published: August 2021

The increase in the role of companion robots in everyday life is inevitable, and their safe communication with the infrastructure is one of the fundamental challenges faced by designers. There are many challenges in the robot’s communication with the environment, widely described in the literature on the subject. The threats that scientists believe have the most significant impact on the robot’s communication include denial-of-service (DoS) attacks, satellite signal spoofing, external eavesdropping, spamming, broadcast tampering, and man-in-the-middle attacks. In this article, the authors attempted to identify communication threats in the new robot-to-infrastructure (R2I) model based on available solutions used in transport, e.g., vehicle-to-infrastructure (V2I), taking into account the threats already known affecting the robot’s sensory systems. For this purpose, all threats that may occur in the robot’s communication with the environment were analyzed. Then the risk analysis was carried out, determining, in turn, the likelihood of potential threats occurrence, their consequence, and ability of detection. Finally, specific methods of responding to the occurring threats are proposed, taking into account cybersecurity aspects. A critical new approach is the proposal to use communication and protocols so far dedicated to transport (IEEE 802.11p WAVE, dedicated short-range communications (DSRC)). Then, the companion’s robot should be treated as a pedestrian and some of its sensors as an active smartphone.
Migration Forecasting—Significance and Approaches
Authors: Fuchs Johann , Söhnlein Doris , Vanella Patrizio
Published: August 2021

Migration is defined as the permanent change in an individual’s usual residence. Forecasting migration is an important requisite for population forecasts or for planning in fields that depend on the future size and structure of the population, such as economics, epidemiology, social insurance, or infrastructure. As migration is the most volatile of all demographic components, its modeling is especially difficult. International migration can be modeled and forecast very differently; users should be familiar with the flaws and strengths of these different approaches.
Post-Materialist Values of Smart City Societies: International Comparison of Public Values for Good Enough Governance
Authors: Lim Seng Boon , Malek Jalaluddin Abdul , Yigitcanlar Tan
Published: August 2021

This study aims to analyze the application of good enough governance in considering the citizens’ value propositions that shape smart city societies. This paper applied a quantitative method with cross-country comparisons. Survey data were derived from the World Values Survey. Malaysia was chosen as the main study area, and compared with Indonesia and other countries worldwide. The findings revealed that politics is the value of least concern across all samples. In terms of qualities for children to develop, respondents in both Malaysia and Indonesia were less concerned about imagination and unselfishness. As for materialist versus post-materialist, the ratios of Malaysia and Indonesia were slightly higher than the average; the post-materialist value of free speech was the lowest value chosen. In the long term, all countries are experiencing the trend of moving toward post-materialist societies. To be sustained under the Collective and Adaptive System of smart city societies, good enough governance in Malaysia and Indonesia should consider the cultural context of the Muslim majority, prioritize governance content that allows more space for political participation and free speech, and cultivate the imagination and unselfishness of children. The generated insights underline the critical role that smart societies play in establishing smart cities.
1-Methylcyclopropene Preserves the Quality of Chive (Allium schoenoprasum L.) by Enhancing Its Antioxidant Capacities and Organosulfur Profile during Storage
Authors: Dai Xiaomei , Lu Yaping , Yang Yuan , Yu Zhifang
Published: August 2021

The quality, antioxidant capacities, and organosulfur profile of chives (Allium schoenoprasum L.) treated with 1-methylcyclopropene (1-MCP) during storage were investigated in this study. The 1-MCP treatment (100 μL/L, fumigation 12 h at 20 °C) effectively inhibited tissue respiration and H2O2 production, enhanced the ascorbic acid (ASA) and glutathione (GSH) content, and promoted the activity of antioxidant enzymes (superoxide dismutase SOD, Catalase CAT, and ascorbic peroxidase APX) during the 5-day storage period at 20 °C. The result further showed that the 1-MCP treatment inhibited chlorophyll degradation, alleviated cell membrane damage, and delayed the chive senescence, with the yellowing rate being reduced by 67.8% and 34.5% in the 1-MCP treated chives on days 4 and 5 of storage at 20 °C, respectively. The free amino acid content of the chive was not affected by the 1-MCP treatment at 20 °C. However, the senescence rate of the chive was not reduced by the 1-MCP treatment when stored at 3 °C. The liquid chromatography data further showed that the 1-MCP treatment induced a 15.3% and 13.9% increase in the isoalliin and total S-alk(en)ylcysteine sulfoxides (ACSOs) content of the chive on day 2 at 20 °C, respectively. Furthermore, there was a strong positive correlation between ACSOs content and CAT/APX activity, indicating that ACSOs probably played a key role in enhancing the antioxidant capacities of the chive during storage at 20 °C. Thus the study efficiently demonstrates that 1-methylcyclopropene preserves the quality of chive (Allium schoenoprasum L.) by enhancing its antioxidant capacities and organosulfur profile during storage.
Sucrose Enhances Anthocyanin Accumulation in Torenia by Promoting Expression of Anthocyanin Biosynthesis Genes
Authors: Naing Aung Htay , Xu Junping , Park Kyeung Il , Chung Mi Young , Kim Chang Kil
Published: August 2021

We examined the effects of different sucrose concentrations (3%, 5%, and 7%) on anthocyanin accumulation and plant growth in wild type (WT) and transgenic (T2) torenia cultivar “Kauai Rose” overexpressing the anthocyanin regulatory transcription factors B-Peru + mPAP1 or RsMYB1. Sucrose increased anthocyanin production in both WT and transgenic plants, with higher anthocyanin production in transgenic plants compared to WT plants. Higher sucrose concentrations increased production of anthocyanin in transgenic and WT plants, with increased anthocyanin production associated with increased expression of anthocyanin biosynthesis genes. Higher sucrose concentrations reduced growth of WT and transgenic plants. Our results indicate that sucrose enhances anthocyanin production in torenia by regulating anthocyanin biosynthesis genes.
Natural Compounds Attenuate Denervation-Induced Skeletal Muscle Atrophy
Authors: Shirakawa Tomohiko , Miyawaki Aki , Kawamoto Tatsuo , Kokabu Shoichiro
Published: August 2021

The weight of skeletal muscle accounts for approximately 40% of the whole weight in a healthy individual, and the normal metabolism and motor function of the muscle are indispensable for healthy life. In addition, the skeletal muscle of the maxillofacial region plays an important role not only in eating and swallowing, but also in communication, such as facial expressions and conversations. In recent years, skeletal muscle atrophy has received worldwide attention as a serious health problem. However, the mechanism of skeletal muscle atrophy that has been clarified at present is insufficient, and a therapeutic method against skeletal muscle atrophy has not been established. This review provides views on the importance of skeletal muscle in the maxillofacial region and explains the differences between skeletal muscles in the maxillofacial region and other regions. We summarize the findings to change in gene expression in muscle remodeling and emphasize the advantages and disadvantages of denervation-induced skeletal muscle atrophy model. Finally, we discuss the newly discovered beneficial effects of natural compounds on skeletal muscle atrophy.
Drosophila Larval Models of Invasive Tumorigenesis for In Vivo Studies on Tumour/Peripheral Host Tissue Interactions during Cancer Cachexia
Authors: Hodgson Joseph , Parvy Jean-Philippe , Yu Yachuan , Vidal Marcos , Cordero Julia
Published: August 2021

Cancer cachexia is a common deleterious paraneoplastic syndrome that represents an area of unmet clinical need, partly due to its poorly understood aetiology and complex multifactorial nature. We have interrogated multiple genetically defined larval Drosophila models of tumourigenesis against key features of human cancer cachexia. Our results indicate that cachectic tissue wasting is dependent on the genetic characteristics of the tumour and demonstrate that host malnutrition or tumour burden are not sufficient to drive wasting. We show that JAK/STAT and TNF-α/Egr signalling are elevated in cachectic muscle and promote tissue wasting. Furthermore, we introduce a dual driver system that allows independent genetic manipulation of tumour and host skeletal muscle. Overall, we present a novel Drosophila larval paradigm to study tumour/host tissue crosstalk in vivo, which may contribute to future research in cancer cachexia and impact the design of therapeutic approaches for this pathology.
Inflammasome Signaling Regulates the Microbial–Neuroimmune Axis and Visceral Pain in Mice
Authors: Aguilera Mònica , Rossini Valerio , Hickey Ana , Simnica Donjete , Grady Fiona , Felice Valeria D. , Moloney Amy , Pawley Lauren , Fanning Aine , McCarthy Lorraine , O’Mahony Siobhan M. , Cryan John F. , Nally Ken , Shanahan Fergus , Melgar Silvia
Published: August 2021

Interactions between the intestinal microbiota, immune system and nervous system are essential for homeostasis in the gut. Inflammasomes contribute to innate immunity and brain–gut interactions, but their role in microbiota–neuro–immune interactions is not clear. Therefore, we investigated the effect of the inflammasome on visceral pain and local and systemic neuroimmune responses after antibiotic-induced changes to the microbiota. Wild-type (WT) and caspase-1/11 deficient (Casp1 KO) mice were orally treated for 2 weeks with an antibiotic cocktail (Abx, Bacitracin A and Neomycin), followed by quantification of representative fecal commensals (by qPCR), cecal short chain fatty acids (by HPLC), pathways implicated in the gut–neuro-immune axis (by RT-qPCR, immunofluorescence staining, and flow cytometry) in addition to capsaicin-induced visceral pain responses. Abx-treatment in WT-mice resulted in an increase in colonic macrophages, central neuro-immune interactions, colonic inflammasome and nociceptive receptor gene expression and a reduction in capsaicin-induced visceral pain. In contrast, these responses were attenuated in Abx-treated Casp1 KO mice. Collectively, the data indicate an important role for the inflammasome pathway in functional and inflammatory gastrointestinal conditions where pain and alterations in microbiota composition are prominent.