bluuebunny/crossref_metadata_2025 huggingface dataset:
Natural Products as Modulators of Nrf2 Signaling Pathway in Neuroprotection
Authors: Moratilla-Rivera Ignacio , Sánchez Marta , Valdés-González Jose Antonio , Gómez-Serranillos María Pilar
Published: February 2023

Neurodegenerative diseases (NDs) affect the West due to the increase in life expectancy. Nervous cells accumulate oxidative damage, which is one of the factors that triggers and accelerates neurodegeneration. However, cells have mechanisms that scavenge reactive oxygen species (ROS) and alleviate oxidative stress (OS). Many of these endogenous antioxidant systems are regulated at the gene expression level by the transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2). In the presence of prooxidant conditions, Nrf2 translocates to the nucleus and induces the transcription of genes containing ARE (antioxidant response element). In recent years, there has been an increase in the study of the Nrf2 pathway and the natural products that positively regulate it to reduce oxidative damage to the nervous system, both in in vitro models with neurons and microglia subjected to stress factors and in vivo models using mainly murine models. Quercetin, curcumin, anthocyanins, tea polyphenols, and other less studied phenolic compounds such as kaempferol, hesperetin, and icariin can also modulate Nrf2 by regulating several Nrf2 upstream activators. Another group of phytochemical compounds that upregulate this pathway are terpenoids, including monoterpenes (aucubin, catapol), diterpenes (ginkgolides), triterpenes (ginsenosides), and carotenoids (astaxanthin, lycopene). This review aims to update the knowledge on the influence of secondary metabolites of health interest on the activation of the Nrf2 pathway and their potential as treatments for NDs.
Evaluation of the Anti-Inflammatory Properties of Mastic Oil Extracted from Pistacia lentiscus var. chia
Authors: Spyridopoulou Katerina , Aindelis Georgios , Kolezaki Georgia , Tiptiri-Kourpeti Angeliki , Chlichlia Katerina
Published: February 2023

Mastic oil (MO) is extracted from the resin of the bark of Pistacia lentiscus var. chia, a tree abundantly grown in the Greek island of Chios. Various biological activities, such as antimicrobial, anticancer and antioxidant, have been associated with the dietary intake of MO. However, little is known about MO’s potential anti-inflammatory effects, while some of its main chemical constituents were reported to exert significant anti-inflammatory activity. This study aims to assay the bioactivity of MO on in vitro and in vivo experimental inflammation models, in particular on LPS-stimulated RAW264.7 macrophages, murine primary peritoneal macrophages and a model of zymosan-induced peritonitis in BALB/c mice. The per os administration of MO inhibited the recruitment of macrophages into the peritoneal cavity of zymosan-treated mice, but did not affect neutrophil mobilisation or the levels of IL-6 or TNF-α in the peritoneal fluid. Similarly, IL-6 and TNF-α secretion in primary LPS-stimulated macrophages was not affected by MO, but the levels of phosphoproteins that activate inflammation in macrophages were differentially regulated. Finally, MO and some of its individual constituents reduced nitric oxide (NO), prostaglandin E2 and TNF-α levels in supernatants of LPS-stimulated RAW264.7 cells and inhibited their phagocytosis rate. Our data imply that MO may promote an anti-inflammatory transition in macrophages due to the combined bioactivities of its individual constituents. Thus, as a mixture of various compounds, MO seems to affect multiple molecular mechanisms that are involved in the development of inflammation. Therefore, more research, focusing on MO’s individual constituents and employing various pre-clinical inflammation models that activate different mechanisms, is required for a detailed investigation of the oil’s potential anti-inflammatory activity.
Development of a Computationally Efficient Model of the Heating Phase in Thermoforming Process Based on the Experimental Radiation Pattern of Heaters
Authors: Hosseinionari Hadi , Ramezankhani Milad , Seethaler Rudolf , Milani Abbas S.
Published: February 2023

In this study, an accurate and computationally efficient model for the heating process of thin thermoplastic sheets during thermoforming is developed. This model opens the door to efficient training of model-free control approaches in thermoforming applications, which often require extensive training data that would be significantly costly and time-consuming to generate using physical setups. This model takes into account heat transfer via radiation between heaters and the sheet, heat transfer via conduction through the sheet, and heat transfer via convection between the sheet and the ambient. In this paper, rather than using an analytical relationship for the view factor, an experiment is designed to determine the exact radiation pattern of the heater on the sheet and the fraction of infrared emission absorbed by the sheet. Comparing the output temperature profile on the sheet from the designed model to IR images from a laboratory-scale heating system indicates that the mean square error is reduced by around four times when compared to traditional models with analytical view factors. Moreover, a comparison of the computation time with COMSOL software for a scenario with the same configuration of computation hardware reveals that the designed model is almost ten times faster.
Fungistatic Activity Mediated by Volatile Organic Compounds Is Isolate-Dependent in Trichoderma sp. “atroviride B”
Authors: van Zijll de Jong Eline , Kandula Janaki , Rostás Michael , Kandula Diwakar , Hampton John , Mendoza-Mendoza Artemio
Published: February 2023

Trichoderma spp. produce multiple bioactive volatile organic compounds (VOCs). While the bioactivity of VOCs from different Trichoderma species is well documented, information on intraspecific variation is limited. The fungistatic activity of VOCs emitted by 59 Trichoderma sp. “atroviride B” isolates against the pathogen Rhizoctonia solani was investigated. Eight isolates representing the two extremes of bioactivity against R. solani were also assessed against Alternaria radicina, Fusarium oxysporum f. sp. lycopersici and Sclerotinia sclerotiorum. VOCs profiles of these eight isolates were analyzed using gas chromatography–mass spectrometry (GC-MS) to identify a correlation between specific VOCs and bioactivity, and 11 VOCs were evaluated for bioactivity against the pathogens. Bioactivity against R. solani varied among the fifty-nine isolates, with five being strongly antagonistic. All eight selected isolates inhibited the growth of all four pathogens, with bioactivity being lowest against F. oxysporum f. sp. lycopersici. In total, 32 VOCs were detected, with individual isolates producing between 19 and 28 VOCs. There was a significant direct correlation between VOC number/quantity and bioactivity against R. solani. 6-pentyl-α-pyrone was the most abundant VOC produced, but 15 other VOCs were also correlated with bioactivity. All 11 VOCs tested inhibited R. solani growth, some by >50%. Some of the VOCs also inhibited the growth of the other pathogens by >50%. This study demonstrates significant intraspecific differences in VOC profiles and fungistatic activity supporting the existence of biological diversity within Trichoderma isolates from the same species, a factor in many cases ignored during the development of biological control agents.
Antifungal Activity of Isavuconazole and Comparator Agents against Contemporaneous Mucorales Isolates from USA, Europe, and Asia-Pacific
Authors: Carvalhaes Cecilia G. , Rhomberg Paul R. , Huband Michael D. , Pfaller Michael A. , Castanheira Mariana
Published: February 2023

Isavuconazole is the only US FDA-approved antifungal for treating invasive mucormycosis. We evaluated isavuconazole activity against a global collection of Mucorales isolates. Fifty-two isolates were collected during 2017–2020 from hospitals located in the USA, Europe, and the Asia-Pacific. Isolates were identified by MALDI-TOF MS and/or DNA sequencing and susceptibility tested by the broth microdilution method following CLSI guidelines. Isavuconazole (MIC50/90, 2/>8 mg/L) inhibited 59.6% and 71.2% of all Mucorales isolates at ≤2 mg/L and ≤4 mg/L, respectively. Among comparators, amphotericin B (MIC50/90, 0.5/1 mg/L) displayed the highest activity, followed by posaconazole (MIC50/90, 0.5/8 mg/L). Voriconazole (MIC50/90, >8/>8 mg/L) and the echinocandins (MIC50/90, >4/>4 mg/L) had limited activity against Mucorales isolates. Isavuconazole activity varied by species and this agent inhibited at ≤4 mg/L 85.2%, 72.7%, and 25% of Rhizopus spp. (n = 27; MIC50/90, 1/>8 mg/L), Lichtheimia spp. (n = 11; MIC50/90, 4/8 mg/L), and Mucor spp. (n = 8; MIC50, >8 mg/L) isolates, respectively. Posaconazole MIC50/90 values against Rhizopus, Lichtheimia, and Mucor species were 0.5/8 mg/L, 0.5/1 mg/L, and 2/- mg/L, respectively; amphotericin B MIC50/90 values were 1/1 mg/L, 0.5/1 mg/L, and 0.5/- mg/L, respectively. As susceptibility profiles varied among Mucorales genera, species identification and antifungal susceptibility testing are advised whenever possible to manage and monitor mucormycosis.
Benzimidazole Derivatives Suppress Fusarium Wilt Disease via Interaction with ERG6 of Fusarium equiseti and Activation of the Antioxidant Defense System of Pepper Plants
Authors: El-Nagar Asmaa , Elzaawely Abdelnaser A. , El-Zahaby Hassan M. , Xuan Tran Dang , Khanh Tran Dang , Gaber Mohamed , El-Wakeil Nadia , El-Sayed Yusif , Nehela Yasser
Published: February 2023

Sweet pepper (Capsicum annuum L.), also known as bell pepper, is one of the most widely grown vegetable crops worldwide. It is attacked by numerous phytopathogenic fungi, such as Fusarium equiseti, the causal agent of Fusarium wilt disease. In the current study, we proposed two benzimidazole derivatives, including 2-(2-hydroxyphenyl)-1-H benzimidazole (HPBI) and its aluminum complex (Al−HPBI complex), as potential control alternatives to F. equiseti. Our findings showed that both compounds demonstrated dose-dependent antifungal activity against F. equiseti in vitro and significantly suppressed disease development in pepper plants under greenhouse conditions. According to in silico analysis, the F. equiseti genome possesses a predicted Sterol 24-C-methyltransferase (FeEGR6) protein that shares a high degree of homology with EGR6 from F. oxysporum (FoEGR6). It is worth mentioning that molecular docking analysis confirmed that both compounds can interact with FeEGR6 from F. equiseti as well as FoEGR6 from F. oxysporum. Moreover, root application of HPBI and its aluminum complex significantly enhanced the enzymatic activities of guaiacol-dependent peroxidases (POX), polyphenol oxidase (PPO), and upregulated four antioxidant-related enzymes, including superoxide dismutase [Cu-Zn] (CaSOD-Cu), L-ascorbate peroxidase 1, cytosolic (CaAPX), glutathione reductase, chloroplastic (CaGR), and monodehydroascorbate reductase (CaMDHAR). Additionally, both benzimidazole derivatives induced the accumulation of total soluble phenolics and total soluble flavonoids. Collectively, these findings suggest that the application of HPBI and Al−HPBI complex induce both enzymatic and nonenzymatic antioxidant defense machinery.
Biodistribution and Adjuvant Effect of an Intranasal Vaccine Based on Chitosan Nanoparticles against Paracoccidioidomycosis
Authors: Santos Júnior Samuel Rodrigues Dos , Barbalho Filipe Vieira , Nosanchuk Joshua D. , Amaral Andre Correa , Taborda Carlos Pelleschi
Published: February 2023

Paracoccidioidomycosis (PCM) is a fungal infection caused by the thermodimorphic Paracoccidioides sp. PCM mainly affects the lungs, but, if it is not contained by the immune response, the disease can spread systemically. An immune response derived predominantly from Th1 and Th17 T cell subsets facilitates the elimination of Paracoccidioides cells. In the present work, we evaluated the biodistribution of a prototype vaccine based on the immunodominant and protective P. brasiliensis P10 peptide within chitosan nanoparticles in BALB/c mice infected with P. brasiliensis strain 18 (Pb18). The generated fluorescent (FITC or Cy5.5) or non-fluorescent chitosan nanoparticles ranged in diameter from 230 to 350 nm, and both displayed a Z potential of +20 mV. Most chitosan nanoparticles were found in the upper airway, with smaller amounts localized in the trachea and lungs. The nanoparticles complexed or associated with the P10 peptide were able to reduce the fungal load, and the use of the chitosan nanoparticles reduced the necessary number of doses to achieve fungal reduction. Both vaccines were able to induce a Th1 and Th17 immune response. These data demonstrates that the chitosan P10 nanoparticles are an excellent candidate vaccine for the treatment of PCM.
Diversity of Fungi Isolated from Potato Nematode Cysts in Guizhou Province, China
Authors: Zhang Hui , Yang Zaifu , Jiang Zhaochun , Zhang Xinyue , Nizamani Mir Muhammad , Wu Yan , Wei Shan , Wang Yong , Xie Xin
Published: February 2023

Potatoes rank third in terms of human consumption after rice and wheat. Globodera spp. are significant pests of potato crop worldwide. Globodera rostochiensis was found in Weining County, Guizhou Province, China, in 2019. We collected soil from the rhizosphere zone from infected potato plants and separated mature cysts through simple floatation and sieving methods. The selected cysts were surface-sterilized, and the colonized fungi were isolated and purified. At the same time, the preliminary identification of fungi and fungi parasites on the cysts of nematodes was carried out. This study aimed to define the species and frequency of fungi-colonizing cysts of G. rostochiensis collected from Weining County, Guizhou Province, China, and provide a basis for the control of G. rostochiensis. As a result, 139 strains of colonized fungi were successfully isolated. Multigene analyses showed that these isolates included 11 orders, 17 families, and 23 genera. The genera Fusarium (with a separation frequency of 59%), Penicillium (11%), Edenia (3.6%), and Paraphaeosphaeria (3.6%) were the most frequently occurring. Among the 44 strains, 27 had a colonization rate of 100% on the cysts of G. rostochiensis. Meanwhile, the functional annotation of 23 genera indicated that some fungi have multitrophic lifestyles combining endophytic, pathogenic, and saprophytic behavior. In conclusion, this study showed the species composition and lifestyle diversity of colonized fungi from G. rostochiensis and demonstrated these isolates as potential sources of biocontrol agents. Colonized fungi were isolated from G. rostochiensis for the first time in China, and the taxonomic diversity of fungi from G. rostochiensis was clarified.
Portfolio Volatility Estimation Relative to Stock Market Cross-Sectional Intrinsic Entropy
Authors: Vințe Claudiu , Ausloos Marcel
Published: February 2023

Selecting stock portfolios and assessing their relative volatility risk compared to the market as a whole, market indices, or other portfolios is of great importance to professional fund managers and individual investors alike. Our research uses the cross-sectional intrinsic entropy (CSIE) model to estimate the cross-sectional volatility of the stock groups that can be considered together as portfolio constituents. The CSIE market volatility estimate is based on daily traded prices—open, high, low, and close (OHLC)—along with the daily traded volume for symbols listed on the considered market. In our study, we benchmark portfolio volatility risks against the volatility of the entire market provided by the CSIE and the volatility of market indices computed using longitudinal data. This article introduces CSIE-based betas to characterise the relative volatility risk of the portfolio against market indices and the market as a whole. We empirically prove that, through CSIE-based betas, multiple sets of symbols that outperform the market indices in terms of rate of return while maintaining the same level of risk or even lower than the one exhibited by the market index can be discovered, for any given time interval. These sets of symbols can be used as constituent stock portfolios and, in connection with the perspective provided by the CSIE volatility estimates, to hierarchically assess their relative volatility risk within the broader context of the overall volatility of the stock market.
A Statistical Analysis of Companies’ Financing Strategies in Portugal during the COVID-19 Pandemic
Authors: Tavares Fernando , Santos Eulália , Vasconcelos Mafalda Venâncio de , Tavares Vasco Capela
Published: February 2023

This study aims to establish which sources of financing were used and the relevance of different banking products for Portuguese companies during the pandemic. We also intend to understand the determinants of companies’ financing options and what lies behind their decisions concerning the appropriate level of debt. A quantitative methodology was used, based on a questionnaire given to Portuguese companies to analyse different financing issues. The sample was composed of 1957 companies with a business volume of more than EUR 500,000 per year. The results show that Portuguese companies focused on managing liquidity and corporate risk. We found evidence that companies kept financing themselves by banking products such as in the pre-pandemic period, although 29.6% resorted to the LAE-COVID economy support line. Companies decide on the appropriate amount of debt based on the nature of the business, the phase of the life cycle in which the company is, the cash flows’ volatility, accounting results, credit rating, and fiscal benefits. Academicians and companies should master the concept of company financing and adopt strategies to consider the level of debt and refine the banking products to be used. Although the literature on business financial management usually claims that all crises are the same, the COVID-19 pandemic not only caused a recession but also forced people and companies to adapt to a new environment. Portuguese companies have shown resilience and focus on their adoption of good financing practices.
Development of Approaches and Organizational Models for the Mass Implementation of Information Modeling Technologies in the Investment and Construction Sphere
Authors: Platonov Anatoly , Larionova Viola , Davy Yury
Published: February 2023

The rapidly increasing use of building information modeling (BIM) technologies in the world is highly relevant to the search for new approaches and managerial models for enterprises in the construction sphere. As shown in the study of several developing countries, there is a certain lag in this area compared with highly industrialized countries. A comparative analysis of countries in terms of the level of spread of BIM technologies was made using open data from job search Internet sites. In this regard, the urgency of the research is due to the need to develop appropriate approaches to intensify the implementation of BIM technologies in the construction and operation of buildings. The purpose of the study is the development of methodological foundations and applied models of functional interaction between participants of construction projects based on BIM. As a working hypothesis, the authors assume that the mass application of BIM technologies is possible in providing a set of measures of different nature: market, non-market, legal, economic, and organizational. The main results of the study provided a solution to the problem of a significant expansion of the scope of BIM technologies in the construction sector through the formation of an information eco-environment for interaction of participants in the project management system.
The Effect of Pyrantel Pamoate Treatment on Fecal Pinworm (Leidynema appendiculata) Parasites of Dietary Dubia Roaches (Blaptica dubia): Efforts to Eliminate Passthrough Fecal Pseudoparasites in Lesser Hedgehog Tenrecs (Echinops telfairi)
Authors: Browder Elizabeth , Kapp Sabrina , Ange-van Heugten Kimberly , Flowers James , Christian Larry S. , Dombrowski Daniel S.
Published: February 2023

Pinworm ova were discovered on lesser hedgehog tenrec (Echinops telfairi) fecal exams. Ova were passthrough pseudoparasite pinworms originating from feeder roaches (Blaptica dubia). Roaches were maintained as a feeder colony and offered to tenrecs as a portion of their diet. Pinworms were identified as Leidynema appendiculata. This study aimed to determine if these pinworms could be eliminated from the roaches. Roaches were randomly assigned into groups (n = 24), including a control (A) and four treatment groups (B–E). Treatment group roaches received oral dosing of anthelmintic pyrantel pamoate at four concentrations (mg/g as offered): 3.5 (Group B), 14.0 (Group C), 26.0 (Group D), and 35.0 (Group E). Roach diets were made weekly and offered to roaches 2 consecutive days per week for 3 consecutive weeks. The total pinworm ova per gram of roach feces examined were visually reduced in all treatment groups compared to controls at the end of the feed dosing period (Day 23). Post-treatment pinworm numbers were visually reduced in all treatment groups compared to controls on Day 29 and Day 65. Groups receiving higher concentrations of the oral dosing (C–E) significantly differed from controls at Day 29 (p = 0.0086, p = 0.0045, and p = 0.0013, respectively) with a concentration-dependent response. Parasites were not eliminated in any group at Day 29 or 65 post-treatment, with an increasing visual trend indicating recontamination. This is the first report confirming a passthrough pseudoparasite in tenrecs from dubia roaches, and anthelmintic dosage research is warranted.
miR-148b as a Potential Biomarker for IgA Nephropathy
Authors: Kumar Santosh , Priscilla C. , Parameswaran Sreejith , Shewade Deepak Gopal , Viswanathan Pragasam , Ganesh Rajesh Nachiappa
Published: February 2023

Background: IgA nephropathy (IgAN) is one of the most common glomerular diseases worldwide. Approximately 25 percent of IgAN patients reach the kidney failure stage within twenty years of diagnosis. The histopathological examination of kidney biopsy is needed to confirm the diagnosis of IgAN. microRNA (miRNA) is a small RNA that plays an important role at the post-transcriptional level by downregulating mRNAs (messenger RNA). We tried to establish a miRNA-based biomarker for IgAN. Methods: We recruited 30 IgAN patients and 15 healthy controls as study participants after taking their informed written consent. A real-time PCR-based method was used for the absolute quantification of miRNAs. A logistic regression method and receiver operating characteristic analysis were performed to find the diagnostic and prognostic accuracy of miR-148b and let-7b for IgAN in histopathological MEST-C scores. Results: miR-148b and let-7b levels were higher in IgAN patients compared to the healthy controls. miR-148b was positively correlated with glomerular filtration rate (GFR) and negatively correlated with segmental glomerulosclerosis, tubular atrophy/interstitial fibrosis (T), and blood pressure (BP). The sensitivity, specificity, and area under the curve (AUC) of the receiver operating characteristic (ROC) for miR-148b against T were 0.87, 0.77, and 0.85, respectively. The threshold value of the miR-148b copy number was 8479 to differentiate the severe condition of IgAN. Conclusion: miR-148b can be used as a potential biomarker for IgAN.
The Unresolved Methodological Challenge of Detecting Neuroplastic Changes in Astronauts
Authors: Burles Ford , Williams Rebecca , Berger Lila , Pike G. Bruce , Lebel Catherine , Iaria Giuseppe
Published: February 2023

After completing a spaceflight, astronauts display a salient upward shift in the position of the brain within the skull, accompanied by a redistribution of cerebrospinal fluid. Magnetic resonance imaging studies have also reported local changes in brain volume following a spaceflight, which have been cautiously interpreted as a neuroplastic response to spaceflight. Here, we provide evidence that the grey matter volume changes seen in astronauts following spaceflight are contaminated by preprocessing errors exacerbated by the upwards shift of the brain within the skull. While it is expected that an astronaut’s brain undergoes some neuroplastic adaptations during spaceflight, our findings suggest that the brain volume changes detected using standard processing pipelines for neuroimaging analyses could be contaminated by errors in identifying different tissue types (i.e., tissue segmentation). These errors may undermine the interpretation of such analyses as direct evidence of neuroplastic adaptation, and novel or alternate preprocessing or experimental paradigms are needed in order to resolve this important issue in space health research.
Ethyl 12-Sulfamoyl-abieta-8,11,13-trien-18-oate
Authors: Izmest’ev Evgeniy S. , Pestova Svetlana V. , Gerasimova Darya P. , Babaeva Olga B. , Lodochnikova Olga A. , Nikitina Liliya E. , Kayumov Airat R. , Rubtsova Svetlana A.
Published: February 2023

We synthesized the novel compound ethyl 12-sulfamoyl-abieta-8,11,13-trien-18-oate in good yield from ethyl 12-sulfo-abieta-8,11,13-trien-18-oate via a two-step protocol. The product was comprehensively characterized by one- and two-dimensional NMR methods, single-crystal X-ray diffraction, IR spectroscopy, and high-resolution mass spectrometry.
Effect of Percutaneous Biliary Drainage on Enzyme Activity of Serum Matrix Metalloproteinase-9 in Patients with Malignant Hilar Obstructive Hyperbilirubinemia
Authors: Filipović Aleksandar , Mašulović Dragan , Gopčević Kristina , Galun Danijel , Igić Aleksa , Bulatović Dušan , Zakošek Miloš , Filipović Tamara
Published: February 2023

Background and Objectives. Cholestasis activates complex mechanisms of liver injury and as a result has an increased production of matrix metalloproteinases (MMP). Depending on the stage of liver disease, different matrix metalloproteinases expressions have been detected and could serve as indirect biomarkers as well as therapeutic targets. MMP-9 proteolytic activity has a proven role in both liver regeneration and neoplastic cell invasion in various malignancies. The purpose of this prospective cohort study was to evaluate the effect of external biliary drainage on enzyme activity of MMP-9 in the serum of patients with malignant hilar biliary obstruction. Materials and Methods. Between November 2020 and April 2021, 45 patients with malignant hilar biliary obstruction underwent percutaneous biliary drainage following determination of serum MMP-9 enzyme activity (before treatment and 4 weeks after the treatment) by gelatin zymography. Results. MMP-9 values decreased statistically significantly 4 weeks after percutaneous biliary drainage (p = 0.028) as well as the value of total bilirubin (p < 0.001), values of direct bilirubin (p < 0.001), aspartate aminotransferase (AST) (p < 0.001), alanine transaminase (ALT) (p < 0.001), and gamma-glutamyl transferase (GGT) (p < 0.001). Conclusions. In patients with malignant hilar biliary obstruction treated by external percutaneous biliary drainage for cholestasis resolution, a significant reduction in MMP-9 serum values was noted 4 weeks after the treatment.
The Relationship between Psychological Suffering, Value of Maternal Cortisol during Third Trimester of Pregnancy and Breastfeeding Initiation
Authors: Răchită Anca Ioana Cristea , Strete Gabriela Elena , Sălcudean Andreea , Ghiga Dana Valentina , Huțanu Adina , Muntean Lorena Mihaela , Suciu Laura Mihaela , Mărginean Claudiu
Published: February 2023

Background and Objectives: Cortisol, the stress hormone, is an important factor in initiating and maintaining lactation. Maternal suffering during pregnancy is predictive for the initiation and shorter duration of breastfeeding and can also lead to its termination. The aim of this study is to evaluate the relationship between the level of salivary cortisol in the third trimester of pregnancy and the initiation of breastfeeding in the postpartum period in a cohort of young pregnant women who wanted to exclusively breastfeed their newborns during hospitalization. Materials and Methods: For the study, full-term pregnant women were recruited between January and May 2022 in the Obstetrics and Gynecology Clinic of the Mureș County Clinical Hospital. Socio-demographic, clinical obstetric and neonatal variables were collected. Breastfeeding efficiency was assessed using the LATCH Breastfeeding Assessment Tool at 24 and 48 h after birth. The mean value of the LATCH score assessed at 24 and 48 h of age was higher among mothers who had a higher mean value of salivary cortisol measured in the third trimester of pregnancy (p < 0.05). A multivariate logistic regression model was used to detect risk factors for the success of early breastfeeding initiation. Results: A quarter of pregnant women had a salivary cortisol level above normal limits during the third trimester of pregnancy. There is a statistically significant association between maternal smoking, alcohol consumption during pregnancy and the level of anxiety or depression. Conclusions: The most important finding of this study was that increased salivary cortisol in the last trimester of pregnancy was not associated with delayed initiation/absence of breastfeeding.
Opportunities of Amlodipine as a Potential Candidate in the Evaluation of Drug Compliance during Antihypertensive Therapy
Authors: Kustovs Dmitrijs , Urtāne Inga , Sevostjanovs Eduards , Moreino Eva , Trušinskis Kārlis
Published: February 2023

Background and Objectives: Blood pressure measurement is essential evidence to establish that the chosen medicine and dosage are appropriate, and also indirectly indicates whether the medicine is being used at all. Therefore, current research compares adherence to the target blood pressure at home and in the hospital between different age groups, using similar combinations of the drugs prescribed by the doctor within ongoing antihypertensive therapy. Moreover, it is very important to develop a method for the determination of amlodipine and its metabolite, which would suitable for clinical applications, when the result is needed as quick as possible. Materials and Methods: This prospective study included patients aged ≥18 years who were diagnosed with hypertension. Subjects were divided into two age groups according to European Society of Cardiology (ESC) hypertension guidelines; older patients (≥65 years) and adult patients (<65 years). Assessment of adherence rate to antihypertensive medications was performed using a measurement of systolic blood pressure and comparing this to ESC hypertension guideline data. A simple liquid chromatography-tandem mass spectrometer (LC-MS/MS) method for determination of amlodipine and dehydroamlodipine was developed and validated according to the European Medicines Agency guideline on bioanalytical method validation at the Latvian Institute of Organic Synthesis. Results: A total of 81 patients with arterial hypertension were enrolled in this study. A significant number of patients were overweight (N = 33, 40.7%) and obese (N = 36, 44.4%). To control arterial hypertension, 70 (86.4%) patients used fixed-dose combinations, where one of the components was amlodipine. Practically, 36 (44.4%) hypertensive subjects were not able to comply with target blood pressure. Nonetheless, 38 (46.9%) patients who received fixed-dose combinations were able to comply with target blood pressure. Conclusions: Adherence to ESC hypertension guideline proposed target blood pressure was relatively low among hypertensive subjects even though a significant number of patients were taking fixed-dose combinations. Therefore, optimizing prevention, recognition, and care of hypertensive young adults require intensive educational interventions. Moreover, survey data suggest that therapeutic drug monitoring using the validated simple, sensitive LC-MS/MS method is pivotal for further understanding factors influencing adherence.
Prediction of Putative Epitope Peptides against BaeR Associated with TCS Adaptation in Acinetobacter baumannii Using an In Silico Approach
Authors: Girija A. S. Smiline , Gunasekaran Shoba , Habib Saman , Aljeldah Mohammed , Al Shammari Basim R. , Alshehri Ahmad A. , Alwashmi Ameen S. S. , Turkistani Safaa A. , Alawfi Abdulsalam , Alshengeti Amer , Garout Mohammed , Alwarthan Sara , Alsubki Roua A. , Moustafa Nouran M. , Rabaan Ali A.
Published: February 2023

Background and Objectives: The BaeR protein is involved in the adaptation system of A. baumannii and is associated with virulence factors responsible for systemic infections in hospitalized patients. This study was conducted to characterize putative epitope peptides for the design of vaccines against BaeR protein, using an immune-informatic approach. Materials and Methods: FASTA sequences of BaeR from five different strains of A. baumannii were retrieved from the UNIPROT database and evaluated for their antigenicity, allergenicity and vaccine properties using BepiPred, Vaxijen, AlgPred, AntigenPro and SolPro. Their physio-chemical properties were assessed using the Expasy Protparam server. Immuno-dominant B-cell and T-cell epitope peptides were predicted using the IEDB database and MHC cluster server with a final assessment of their interactions with TLR-2. Results: A final selection of two peptide sequences (36aa and 22aa) was made from the 38 antigenic peptides. E1 was considered a soluble, non-allergenic antigen, and possessed negative GRAVY values, substantiating the hydrophilic nature of the proteins. Further analysis on the T-cell epitopes, class I immunogenicity and HLA allele frequencies yielded T-cell immuno-dominant peptides. The protein–peptide interactions of the TLR-2 receptor showed good similarity scores in terms of the high number of hydrogen bonds compared to other protein-peptide interactions. Conclusions: The two epitopes predicted from BaeR in the present investigation are promising vaccine candidates for targeting the TCS of A. baumannii in systemic and nosocomial infections. This study also demonstrates an alternative strategy to tackling and mitigating MDR strains of A. baumannii and provides a useful reference for the design and construction of novel vaccine candidates against this bacteria.
Urinary microRNA in Diabetic Kidney Disease: A Literature Review
Authors: Lee Chin-Chan , Chen Chia-Chun , Hsu Cheng-Kai , Chen Yih-Ting , Chen Chun-Yu , Yang Kai-Jie , Hung Ming-Jui , Wu I-Wen
Published: February 2023

Diabetic kidney disease is the most common primary disease of end-stage kidney disease globally; however, a sensitive and accurate biomarker to predict this disease remains awaited. microRNAs are endogenous single-stranded noncoding RNAs that have intervened in different post-transcriptional regulations of various cellular biological functions. Previous literatures have reported its potential role in the pathophysiology of diabetic kidney disease, including regulation of Transforming Growth Factor-β1-mediated fibrosis, extracellular matrix and cell adhesion proteins, cellular hypertrophy, growth factor, cytokine production, and redox system activation. Urinary microRNAs have emerged as a novel, non-invasive liquid biopsy for disease diagnosis. In this review, we describe the available experimental and clinical evidence of urinary microRNA in the context of diabetic kidney disease and discuss the future application of microRNA in routine practice.
Transcriptome-Wide Analysis of microRNA–mRNA Correlations in Tissue Identifies microRNA Targeting Determinants
Authors: Trinidad-Barnech Juan Manuel , Fort Rafael Sebastián , Trinidad Barnech Guillermo , Garat Beatriz , Duhagon María Ana
Published: February 2023

MicroRNAs are small RNAs that regulate gene expression through complementary base pairing with their target mRNAs. A substantial understanding of microRNA target recognition and repression mechanisms has been reached using diverse empirical and bioinformatic approaches, primarily in vitro biochemical or cell culture perturbation settings. We sought to determine if rules of microRNA target efficacy could be inferred from extensive gene expression data of human tissues. A transcriptome-wide assessment of all the microRNA–mRNA canonical interactions’ efficacy was performed using a normalized Spearman correlation (Z-score) between the abundance of the transcripts in the PRAD-TCGA dataset tissues (RNA-seq mRNAs and small RNA-seq for microRNAs, 546 samples). Using the Z-score of correlation as a surrogate marker of microRNA target efficacy, we confirmed hallmarks of microRNAs, such as repression of their targets, the hierarchy of preference for gene regions (3′UTR > CDS > 5′UTR), and seed length (6 mer < 7 mer < 8 mer), as well as the contribution of the 3′-supplementary pairing at nucleotides 13–16 of the microRNA. Interactions mediated by 6 mer + supplementary showed similar inferred repression as 7 mer sites, suggesting that the 6 mer + supplementary sites may be relevant in vivo. However, aggregated 7 mer-A1 seeds appear more repressive than 7 mer-m8 seeds, while similar when pairing possibilities at the 3′-supplementary sites. We then examined the 3′-supplementary pairing using 39 microRNAs with Z-score-inferred repressive 3′-supplementary interactions. The approach was sensitive to the offset of the bridge between seed and 3′-supplementary pairing sites, and the pattern of offset-associated repression found supports previous findings. The 39 microRNAs with effective repressive 3′supplementary sites show low GC content at positions 13–16. Our study suggests that the transcriptome-wide analysis of microRNA–mRNA correlations may uncover hints of microRNA targeting determinants. Finally, we provide a bioinformatic tool to identify microRNA–mRNA candidate interactions based on the sequence complementarity of the seed and 3′-supplementary regions.
Reduction of Alcoholic Strength: Does It Matter for Public Health?
Authors: Rehm Jürgen , Rovira Pol , Manthey Jakob , Anderson Peter
Published: February 2023

In this work, reduction of alcoholic strength was discussed as a means to reduce consumption and alcohol-attributable harm. Statistical modelling was conducted to (1) estimate its potential for the largest six Western and Central European countries (France, Germany, Italy, Poland, Spain, UK); (2) calculate the increase in taxation necessary to reach this potential, and (3) estimate the mortality gains achieved with the introduction of no- or low-alcohol beverages in the UK and Spain. The high public health potential of reducing alcoholic strength was demonstrated via modelling a scenario in which the strength of all beverages was reduced by 10%, which would avert thousands of deaths in these six European countries per year. However, methods by which to achieve these gains were not clear, as the alcohol industry has shown no inclination toward reductions in the alcoholic strength of beer, wine, or spirits via a reformulation on a large scale. The increase of excise taxation to achieve the public health gains of such a reduction would result in markedly increasing prices—a situation unlikely to be implemented in Europe. Finally, the introduction of beer and wine with an alcoholic strength below 0.5% led to some substitutions of higher-strength beverages, but did not show a marked public health impact. New taxation initiatives to achieve the potential of a reduction of alcoholic strength will need to be implemented.
Why Are Obese People Predisposed to Severe Disease in Viral Respiratory Infections?
Authors: Aziz Rafia , Sherwani Afak Yusuf , Al Mahri Saeed , Malik Shuja Shafi , Mohammad Sameer
Published: February 2023

Obesity is one of the most pressing healthcare concerns of the twenty-first century. Obesity prevalence has risen dramatically in recent decades, and in 2016, more than 1.9 billion adults were overweight (BMI ≥ 25 kg/m2) and 650 million were obese (BMI ≥ 30 kg/m2). About 50% of the world’s population is anticipated to be obese/overweight within the next decade. Obesity is a major risk factor for a variety of non-communicable diseases, including type 2 diabetes mellitus, cardiovascular disease, non-alcoholic fatty liver disease, and a variety of malignancies. Obesity has emerged as a substantial risk factor for hospitalization and death from viral respiratory infections such as influenza A and the ongoing pandemic SARS-CoV-2. Several independent studies have indicated that obese/overweight patients are at a higher risk of severe disease and death from these respiratory diseases. Excess fat, particularly visceral fat, contributes to the development of a variety of metabolic disorders, including persistent systemic inflammation and decreased immunological function. As a result, the immunological response to infectious pathogens is weakened, resulting in poorer outcomes post-infection. Additionally, the poor lung mechanics associated with obesity may increase the risk of more serious respiratory infections. In this review, we address the likely mechanism(s) that predispose obese people to severe diseases caused by viral respiratory infections.
Generation of 48 fs, 1 GHz Fundamentally Mode-Locked Pulses Directly from an Yb-doped “Solid-State Fiber Laser”
Authors: Hu Mengyun , Shen Jiawei , Cao Yuzhi , Yuan Shuai , Zeng Heping
Published: February 2023

We demonstrate a fundamentally mode-locked Yb-doped “solid-state fiber laser” with a repetition rate of 1 GHz and a pulse duration of 48 fs. The nonlinear-polarization-evolution (NPE) mode-locking of the “solid-state fiber laser” enables up to 286 mW of average power and a 26 nm spectrum bandwidth, which supports a 48 fs pulse duration. The laser self-starts and the central wavelength can be tuned from 1032.4 nm to 1035.6 nm. To the best of our knowledge, it is the shortest pulse duration directly obtained by GHz fundamentally mode-locked Yb-fiber lasers.
Optical Bottle Shaping Using Axicons with Amplitude or Phase Apodization
Authors: Khonina Svetlana N. , Ustinov Andrey V. , Kharitonov Sergey I. , Fomchenkov Sergey A. , Porfirev Alexey P.
Published: February 2023

We investigate the formation of single and multiple optical bottle beams on the optical axis using a diffractive axicon with amplitude or phase apodization. The proposed approach allows one to control the location and the contrast of the boundaries of the generated dark intensity regions on the optical axis. Experimental results obtained using a spatial light modulator are in good agreement with numerically obtained ones. We successfully used the designed and experimentally formed set of three optical bottle beams for trapping light-absorbing agglomerations of carbon nanoparticles in air under the action of photophoretic forces. This confirms the efficiency of the proposed approach for optical manipulation applications.
Research on the Biomechanical Characteristics of Salted Wakame (Undaria pinnatifida)
Authors: Zhang Hanbing , Huang Weirong , Kang Huanyu , Wu Shuqiao , Li Xiuchen
Published: February 2023

At present, there has been no mechanized equipment designed for the separation of the leaves from the stems of wakame in China, and the stem–leaf separation is mainly completed by manual work, which suffers from problems such as low efficiency, high cost, and poor quality. To develop mechanized stem–leaf separation equipment of wakame, it is necessary to have a preliminary understanding of the biomechanical characteristics of wakame. In this study, we adopted an electronic universal testing machine, a texture tester, and a friction tester to investigate the mechanical characteristics of the stems and leaves of salted wakame. Analysis was performed to clarify the effects of salted wakame thickness and loading speed on the tension, compression, shear, and separation mechanical properties as well as the effects of the loading speed, normal force, and contact material on the friction characteristics. As shown in the results, the average tensile strength, shear strength, and resilience of salted wakame stems were 2.27 MPa, 6.34 MPa and 0.27, respectively; the average tensile strength and shear strength of salted wakame leaves were 1.67 MPa and 2.93 MPa; the separation strength of the stems and leaves was 1.78 MPa, and the friction coefficient between salted wakame and stainless steel, silicone rubber, and vulcanized rubber was 0.38, 0.44 and 0.40 on average. In general, the increase in the loading rate, the shear strength, and the stem–leaf separation strength of salted wakame showed a downward trend, while the changes in the friction coefficient showed an upward trend, with no significant influence on the recovery and tensile strength. The tensile strength, resilience, shear strength, stem–leaf separation force, and strength increased as the thickness of the salted wakame improved. The friction coefficient of the salted wakame stems decreased with increasing the normal force, while the friction coefficient of the salted wakame leaves increased. In general, the increase in wakame thickness improved its mechanical properties, and the increase in the test loading rate led to the decrease in the mechanical properties of salted wakame. The research results in this paper can provide suggestions for the research and development of stem–leave separation equipment for saline salted wakame.
Identification of Streamside Landslides with the Use of Unmanned Aerial Vehicles (UAVs) in Greece, Romania, and Turkey
Authors: Yavuz Mehmet , Koutalakis Paschalis , Diaconu Daniel Constantin , Gkiatas Georgios , Zaimes George N. , Tufekcioglu Mustafa , Marinescu Maria
Published: February 2023

The alleviation of landslide impacts is a priority since they have the potential to cause significant economic damage as well as the loss of human life. Mitigation can be achieved effectively by using warning systems and preventive measures. The development of improved methodologies for the analysis and understanding of landslides is at the forefront of this scientific field. Identifying effective monitoring techniques (accurate, fast, and low cost) is the pursued objective. Geographic Information Systems (GISs) and remote sensing techniques are utilized in order to achieve this goal. In this study, four methodological approaches (manual landslide delineation, a segmentation process, and two mapping models, specifically object-based image analysis and pixel-based image analysis (OBIA and PBIA)) were proposed and tested with the use of Unmanned Aerial Vehicles (UAVs) and data analysis methods to showcase the state and evolution of landslides. The digital surface model (DSM)-based classification approach was also used to support the aforementioned approaches. This study focused on streamside landslides at research sites in three different countries: Greece, Romania, and Turkey. The results highlight that the areas of the OBIA-based classifications were the most similar (98%) to our control (manual) classifications for all three sites. The landslides’ perimeters at the Lefkothea and Chirlesti sites showed similar results to the OBIA-based classification (93%), as opposed to the Sirtoba site, where the perimeters of the landslides from OBIA-based classification were not well corroborated by the perimeters in the manual classification. Deposition areas that extend beyond the trees were revealed by the DSM-based classification. The results are encouraging because the methodology can be used to monitor landslide evolution with accuracy and high performance in different regions. Specifically, terrains that are difficult to access can be surveyed by UAVs because of their ability to take aerial images. The obtained results provide a framework for the unitary analysis of landslides using modern techniques and tools.