bluuebunny/crossref_metadata_2025 huggingface dataset:
MAPK Cascades and Transcriptional Factors: Regulation of Heavy Metal Tolerance in Plants
Authors: Li Shaocui , Han Xiaojiao , Lu Zhuchou , Qiu Wenmin , Yu Miao , Li Haiying , He Zhengquan , Zhuo Renying
Published: April 2022

In nature, heavy metal (HM) stress is one of the most destructive abiotic stresses for plants. Heavy metals produce toxicity by targeting key molecules and important processes in plant cells. The mitogen-activated protein kinase (MAPK) cascade transfers the signals perceived by cell membrane surface receptors to cells through phosphorylation and dephosphorylation and targets various effector proteins or transcriptional factors so as to result in the stress response. Signal molecules such as plant hormones, reactive oxygen species (ROS), and nitric oxide (NO) can activate the MAPK cascade through differentially expressed genes, the activation of the antioxidant system and synergistic crosstalk between different signal molecules in order to regulate plant responses to HMs. Transcriptional factors, located downstream of MAPK, are key factors in regulating plant responses to heavy metals and improving plant heavy metal tolerance and accumulation. Thus, understanding how HMs activate the expression of the genes related to the MAPK cascade pathway and then phosphorylate those transcriptional factors may allow us to develop a regulation network to increase our knowledge of HMs tolerance and accumulation. This review highlighted MAPK pathway activation and responses under HMs and mainly focused on the specificity of MAPK activation mediated by ROS, NO and plant hormones. Here, we also described the signaling pathways and their interactions under heavy metal stresses. Moreover, the process of MAPK phosphorylation and the response of downstream transcriptional factors exhibited the importance of regulating targets. It was conducive to analyzing the molecular mechanisms underlying heavy metal accumulation and tolerance.
Chaperone Hsp70 (HSPA1) Is Involved in the Molecular Mechanisms of Sleep Cycle Integration
Authors: Simonova Valentina V. , Guzeev Mikhail A. , Ekimova Irina V. , Pastukhov Yuri F.
Published: April 2022

The molecular mechanisms of sleep cycle integration at the beginning and the end of the inactive period are not clear. Sleep cycles with a predominance of deep slow-wave sleep (SWS) seem to be associated with accelerated protein synthesis in the brain. The inducible Hsp70 chaperone corrects protein conformational changes and has protective properties. This research explores (1) whether the Hspa1 gene encoding Hsp70 protein activates during the daily rapid-eye-movement sleep (REMS) maximum, and (2) whether a lower daily deep SWS maximum affects the Hspa1 expression level during the subsequent REMS. Combining polysomnography in male Wistar rats, RT-qPCR, and Western blotting, we reveal a three-fold Hspa1 upregulation in the nucleus reticularis pontis oralis, which regulates REMS. Hspa1 expression increases during the daily REMS maximum, 5–7 h after the natural peak of deep SWS. Using short-term selective REMS deprivation, we demonstrate that REMS rebound after deprivation exceeds the natural daily maximum, but it is not accompanied by Hspa1 upregulation. The results suggest that a high proportion of deep SWS, usually observed after sleep onset, is a necessary condition for Hspa1 upregulation during subsequent REMS. The data obtained can inform the understanding of the molecular mechanisms integrating SWS and REMS and key biological function(s) of sleep.
Functional Characterization of Cardiac Actin Mutants Causing Hypertrophic (p.A295S) and Dilated Cardiomyopathy (p.R312H and p.E361G)
Authors: Hassoun Roua , Erdmann Constanze , Schmitt Sebastian , Fujita-Becker Setsuko , Mügge Andreas , Schröder Rasmus R. , Geyer Matthias , Borbor Mina , Jaquet Kornelia , Hamdani Nazha , Mannherz Hans Georg
Published: April 2022

Human wild type (wt) cardiac α-actin and its mutants p.A295S or p.R312H and p.E361G correlated with hypertrophic or dilated cardiomyopathy, respectively, were expressed by using the baculovirus/Sf21 insect cell system. The c-actin variants inhibited DNase I, indicating maintenance of their native state. Electron microscopy showed the formation of normal appearing actin filaments though they showed mutant specific differences in length and straightness correlating with their polymerization rates. TRITC-phalloidin staining showed that p.A295S and p.R312H exhibited reduced and the p.E361G mutant increased lengths of their formed filaments. Decoration of c-actins with cardiac tropomyosin (cTm) and troponin (cTn) conveyed Ca2+-sensitivity of the myosin-S1 ATPase stimulation, which was higher for the HCM p.A295S mutant and lower for the DCM p.R312H and p.E361G mutants than for wt c-actin. The lower Ca2+-sensitivity of myosin-S1 stimulation by both DCM actin mutants was corrected by the addition of levosimendan. Ca2+-dependency of the movement of pyrene-labeled cTm along polymerized c-actin variants decorated with cTn corresponded to the relations observed for the myosin-S1 ATPase stimulation though shifted to lower Ca2+-concentrations. The N-terminal C0C2 domain of cardiac myosin-binding protein-C increased the Ca2+-sensitivity of the pyrene-cTM movement of bovine, recombinant wt, p.A295S, and p.E361G c-actins, but not of the p.R312H mutant, suggesting decreased affinity to cTm.
Coinfection of Porcine Circovirus 2 and Pseudorabies Virus Enhances Immunosuppression and Inflammation through NF-κB, JAK/STAT, MAPK, and NLRP3 Pathways
Authors: Li Xue , Chen Si , Zhang Liying , Niu Guyu , Zhang Xinwei , Yang Lin , Ji Weilong , Ren Linzhu
Published: April 2022

Porcine circovirus 2 (PCV2) and pseudorabies virus (PRV) are economically important pathogens in swine. PCV2 and PRV coinfection can cause more severe neurological and respiratory symptoms and higher mortality of piglets. However, the exact mechanism involved in the coinfection of PRV and PCV2 and its pathogenesis remain unknown. Here, porcine kidney cells (PK-15) were infected with PCV2 and/or PRV, and then the activation of immune and inflammatory pathways was evaluated to clarify the influence of the coinfection on immune and inflammatory responses. We found that the coinfection of PCV2 and PRV can promote the activation of nuclear factor-κB (NF-κB), c-Jun N-terminal protein kinases (JNK), p38, and nod-like receptor protein 3 (NLRP3) pathways, thus enhancing the expression of interferon-γ (IFN-γ), interferon-λ1 (IFN-λ1), interferon-stimulated gene (ISG15), interleukin 6 (IL6), and interleukin 1β (IL1β). Meanwhile, PCV2 and PRV also inhibit the expression and signal transduction of IFN-β, tumor necrosis factor α (TNFα), and the Janus kinase-signal transducer and activator of transcription (JAK/STAT) pathway. In addition, PCV2 and PRV infection can also weaken extracellular-signal-regulated kinase (ERK) activity. These results indicate that the regulations of cellular antiviral immune responses and inflammatory responses mediated by NF-κB, JAK/STAT, mitogen-activated protein kinase (MAPK), and NLRP3 pathways, contribute to immune escape of PCV2 and PRV and host antiviral responses.
Medical Knowledge Graph Completion Based on Word Embeddings
Authors: Gao Mingxia , Lu Jianguo , Chen Furong
Published: April 2022

The aim of Medical Knowledge Graph Completion is to automatically predict one of three parts (head entity, relationship, and tail entity) in RDF triples from medical data, mainly text data. Following their introduction, the use of pretrained language models, such as Word2vec, BERT, and XLNET, to complete Medical Knowledge Graphs has become a popular research topic. The existing work focuses mainly on relationship completion and has rarely solved entities and related triples. In this paper, a framework to predict RDF triples for Medical Knowledge Graphs based on word embeddings (named PTMKG-WE) is proposed, for the specific use for the completion of entities and triples. The framework first formalizes existing samples for a given relationship from the Medical Knowledge Graph as prior knowledge. Second, it trains word embeddings from big medical data according to prior knowledge through Word2vec. Third, it can acquire candidate triples from word embeddings based on analogies from existing samples. In this framework, the paper proposes two strategies to improve the relation features. One is used to refine the relational semantics by clustering existing triple samples. Another is used to accurately embed the expression of the relationship through means of existing samples. These two strategies can be used separately (called PTMKG-WE-C and PTMKG-WE-M, respectively), and can also be superimposed (called PTMKG-WE-C-M) in the framework. Finally, in the current study, PubMed data and the National Drug File-Reference Terminology (NDF-RT) were collected, and a series of experiments was conducted. The experimental results show that the framework proposed in this paper and the two improvement strategies can be used to predict new triples for Medical Knowledge Graphs, when medical data are sufficiently abundant and the Knowledge Graph has appropriate prior knowledge. The two strategies designed to improve the relation features have a significant effect on the lifting precision, and the superposition effect becomes more obvious. Another conclusion is that, under the same parameter setting, the semantic precision of word embedding can be improved by extending the breadth and depth of data, and the precision of the prediction framework in this paper can be further improved in most cases. Thus, collecting and training big medical data is a viable method to learn more useful knowledge.
Natural Repellents as a Method of Preventing Ant Damage to Microirrigation Systems
Authors: de Pedro Luis , Sanchez Juan Antonio
Published: April 2022

Ants are important because they damage agricultural equipment, including microirrigation systems. The aim of this research was to assess the efficiency of the incorporation of repellents in drip irrigation tubing as a method of protection against ant damage. Unlike previous studies, we tested a series of nontoxic compounds that are repellent to ants. First, we assessed their repellent effects on a local ant species via olfactometer trials. Then, the candidates showing the best results (cinnamon essential oil, p-anisaldehyde and ethyl anthranilate) were incorporated via compounding, injection and extrusion to polyethylene tubing to test their efficiency in the field. Field tests showed high damage levels in the control tubing containing no repellents, presumably caused by up to six different ant species (Cardiocondyla batesii, Plagiolepis pygmaea, P. schmitzii, Solenopsis sp., Tapinoma nigerrimum and Tetramorium semilaeve). In contrast, the pipes containing the three selected compounds remained almost intact, with the treatment including ethyl anthranilate showing no damage at all. These results suggest the strong repellent potential of the selected compounds, even when integrated into plastic, as well as the apparent success of the proposed methodology against the damage caused by ants. The diversity of damage-causing agents that exist in or above the soil strongly encourages further studies to determine the overall efficiency of repellents in protecting irrigation pipes.
Effect of Physical Exercise on Sleep Quality in Elderly Adults: A Systematic Review with a Meta-Analysis of Controlled and Randomized Studies
Authors: Silva Vitor P. O. , Silva Marcelo P. O. , Silva Vitor L. de S. , Mantovani David B. C. , Mittelmann João V. , Oliveira João V. V. , Pessoa João P. de L. , Chaves Yuri L. , Haddad Mikhail P. , Andrielli Otávio , Bento Vinícius L. , Dourado Mayara L. C. , Melo Hugo M. de A.
Published: April 2022

Introduction: Aging is directly related to sleep problems. Primary insomnia has a negative impact on the lives of elderly adults, altering cognitive and metabolic functions. Physical activity is positively related to improvement in sleep quality. The objective of this systematic review was to analyze the effects of physical activity programs in healthy elderly individuals aged 60 years or older, using the Pittsburgh Sleep Quality Index (PSQI) as a tool. Methods: The search was performed in the PubMed and Scielo databases, July 2021. Only randomized clinical trials that evaluated the role of physical exercise in the sleep quality of elderly patients were selected by two independent reviewers. Results: The result of the PSQI analysis showed that compared with the control condition, the exercise intervention was beneficial for the groups with insomnia (SMD: −0.57; 95% CI: −0.73 to −0.4; p < 0.00001; I2 = 53%) and without insomnia (SMD: −0.61; 95% CI: −0.75 to −0.47; p < 0.00001; I2 = 73%) and for the two groups combined (SMD: −0.59; 95% CI: −0.70 to −0.49; p < 0.0001, I2 = 68%). Conclusion: The systematic and continuous practice of physical exercise significantly improves perceived sleep quality in elderly individuals. Therefore, physical activities can be used as a tool to prevent sleep disorders and improve health in general. Future studies may clarify the comparison between aerobic and resistance exercises, evaluate the dose–response relationship and include more participants.
Corneal Confocal Microscopy Identifies People with Type 1 Diabetes with More Rapid Corneal Nerve Fibre Loss and Progression of Neuropathy
Authors: Alam Uazman , Ponirakis Georgios , Asghar Omar , Petropoulos Ioannis N. , Azmi Shazli , Jeziorska Maria , Marshall Andrew , Boulton Andrew J. M. , Efron Nathan , Malik Rayaz A.
Published: April 2022

There is a need to accurately identify patients with diabetes at higher risk of developing and progressing diabetic peripheral neuropathy (DPN). Fifty subjects with Type 1 Diabetes Mellitus (T1DM) and sixteen age matched healthy controls underwent detailed neuropathy assessments including symptoms, signs, quantitative sensory testing (QST), nerve conduction studies (NCS), intra epidermal nerve fiber density (IENFD) and corneal confocal microscopy (CCM) at baseline and after 2 years of follow-up. Overall, people with type 1 diabetes mellitus showed no significant change in HbA1c, blood pressure, lipids or neuropathic symptoms, signs, QST, neurophysiology, IENFD and CCM over 2 years. However, a sub-group (n = 11, 22%) referred to as progressors, demonstrated rapid corneal nerve fiber loss (RCNFL) with a reduction in corneal nerve fiber density (CNFD) (p = 0.0006), branch density (CNBD) (p = 0.0002), fiber length (CNFL) (p = 0.0002) and sural (p = 0.04) and peroneal (p = 0.05) nerve conduction velocities, which was not related to a change in HbA1c or cardiovascular risk factors. The majority of people with T1DM and good risk factor control do not show worsening of neuropathy over 2 years. However, CCM identifies a sub-group of people with T1DM who show a more rapid decline in corneal nerve fibers and nerve conduction velocity.
Impact of Female Sex on Outcomes of Patients Undergoing Thoracic Endovascular Aortic Aneurysm Repair: A Ten-Year Retrospective Nationwide Study in France
Authors: Lareyre Fabien , Raffort Juliette , Behrendt Christian-Alexander , Chaudhuri Arindam , Lê Cong Duy , Fabre Roxane , Pradier Christian , Bailly Laurent
Published: April 2022

The impact of sex on the outcomes of patients with cardiovascular disease is still incompletely understood. The aim of this nationwide multicenter observational study was to investigate the impact of sex on post-operative outcomes in patients undergoing thoracic endovascular aortic repair (TEVAR) for intact thoracic aortic aneurysm (iTAA). The French National Health Insurance Information System was searched to identify these patients over a ten-year retrospective period. Post-operative outcomes, 30-day and overall mortality were recorded. Among the 7383 patients included (5521 men and 1862 women), females were significantly older than males (66.8 vs. 64.8 years, p < 0.001). They were less frequently diagnosed with cardiovascular comorbidities. Post-operatively, women had less frequently respiratory (10.9 vs. 13.7%, p = 0.002) as well as cardiac complications (34.3 vs. 37.3%, p = 0.023), but they had more frequently arterial complications (52.8 vs. 49.8%, p = 0.024). There was no significant difference on overall mortality for a mean follow-up of 2.2 years (26.9 vs. 27.6%, p = 0.58). In the multivariable regression model, female sex was not associated with 30-day or overall mortality. Although women had a favorable comorbidity profile, the short-term and long-term survival was similar. The significantly higher rate of arterial complications suggests that women may be at higher risk of access-vessel-related complications.
Simultaneous Comparison of Subxiphoid and Intercostal Wound Pain in the Same Patients Following Thoracoscopic Surgery
Authors: Liu Yu-Wei , Chou Shah-Hwa , Chou Andre , Kao Chieh-Ni
Published: April 2022

There is a lack of data comparing postoperative pain after subxiphoid and intercostal video-assisted thoracoscopic surgery (VATS). Pain is an individual’s subjective experience and, therefore, difficult to compare between different individuals subjected to either procedure. This study assessed reported pain at six postoperative time points in the same patients receiving both subxiphoid and intercostal incisions for thoracic disease. Data from 44 patients who received simultaneous combined intercostal and subxiphoid VATS were retrospectively analyzed from August 2019 to July 2021. All patients received the same length of subxiphoid and intercostal incisions with or without drain placements. A numerical pain rating scale was administered on postoperative days (POD)-1, POD-2, POD-Discharge, POD-30, POD-90, and POD-180. Bilateral uniportal VATS was performed in 11 patients, and unilateral multiportal VATS was performed in 33 patients. In the unilateral VATS group, there were no differences in pain reported for both incisions in the early postoperative period. However, in the bilateral VATS group, subxiphoid wounds resulted in significantly higher pain scores on POD-1, POD-2, and POD-Discharge (p = 0.0003, 0.001, and 0.03, respectively). Higher late (3 and 6 months) postoperative pain was associated with intercostal incisions in both groups, as previously reported, whereas higher early (day 1, 2, and discharge) postoperative pain was more associated with subxiphoid incisions than intercostal incisions in the bilateral VATS group.
Impact of Deprivation on Obesity in Children with PWS
Authors: Grolleau Sabrina , Delagrange Marine , Souquiere Melina , Molinas Catherine , Diene Gwenaëlle , Valette Marion , Tauber Maithé
Published: April 2022

Our study aimed to evaluate the social deprivation score in families with a child with Prader-Willi syndrome (PWS) and analyze its impact on the occurrence of obesity in the affected child. We included 147 children with PWS followed in our reference center with Evaluation of the Deprivation and Inequalities of Health in Healthcare Centres by the EPICES score. Deprivation (EPICES ≥ 30) was found in 25.9% of the population. Compared with the non-obese children, children with obesity had more deprived families, 50.0 vs. 18.0% (p = 0.0001); were older, with a median of 10.1 vs. 6.0 years (p = 0.0006); were less frequently treated with growth hormone (GH), 80.6 vs. 91.9% (p = 0.07). The mothers of obese children were more frequently obese, 46.9 vs. 13.3% (p < 0.0001), and achieved high study levels less frequently (≥Bac+2), 40.9 vs. 70.1% (p = 0.012). The multivariate logistic regression indicated that age, living in a deprived family, and having a mother with overweight/obesity were significantly associated with an increased risk of obesity (respectively, OR = 3.31 (1.26–8.73) and OR = 6.76 (2.36–19.37)). The same risk factors of obesity observed in the general population were found in children with PWS. Families at risk, including social deprivation, will require early identification and a reinforced approach to prevent obesity.
Usefulness of Multi-Organ Point-of-Care Ultrasound as a Complement to the Decision-Making Process in Internal Medicine
Authors: Casado-López Irene , Tung-Chen Yale , Torres-Arrese Marta , Luordo-Tedesco Davide , Mata-Martínez Arantzazu , Casas-Rojo Jose Manuel , Montero-Hernández Esther , García De Casasola-Sánchez Gonzalo
Published: April 2022

Accumulated data show the utility of diagnostic multi-organ point-of-care ultrasound (PoCUS) in the assessment of patients admitted to an internal medicine ward. We assessed whether multi-organ PoCUS (lung, cardiac, and abdomen) provides relevant diagnostic and/or therapeutic information in patients admitted for any reason to an internal medicine ward. We conducted a prospective, observational, and single-center study, at a secondary hospital. Multi-organ PoCUS was performed during the first 24 h of admission. The sonographer had access to the patients’ medical history, physical examination, and basic complementary tests performed in the Emergency Department (laboratory, X-ray, electrocardiogram). We considered a relevant ultrasound finding if it implied a significant diagnostic and/or therapeutic change. In the second semester of 2019, we enrolled 310 patients, 48.7% were male and the mean age was 70.5 years. Relevant ultrasound findings were detected in 86 patients (27.7%) and in 60 (19.3%) triggered a therapeutic change. These findings were associated with an older age (Mantel–Haenszel χ2 = 25.6; p < 0.001) and higher degree of dependency (Mantel–Haenszel χ2 = 5.7; p = 0.017). Multi-organ PoCUS provides relevant diagnostic information, complementing traditional physical examination, and facilitates therapy adjustment, regardless of the cause of admission. Multi-organ PoCUS to be useful need to be systematically integrated into the decision-making process in internal medicine.
The Role of the Immune System in Pediatric Burns: A Systematic Review
Authors: Korzeniowski Tomasz , Mertowska Paulina , Mertowski Sebastian , Podgajna Martyna , Grywalska Ewelina , Strużyna Jerzy , Torres Kamil
Published: April 2022

Burns are one of the most common causes of home injuries, characterized by serious damage to the skin and causing the death of affected tissues. In this review, we intended to collect information on the pathophysiological effects of burns in pediatric patients, with particular emphasis on local and systemic responses. A total of 92 articles were included in the review, and the time range of the searched articles was from 2000 to 2021. The occurrence of thermal injuries is a problem that requires special attention in pediatric patients who are still developing. Their exposure to various burns may cause disturbances in the immune response, not only in the area of tissue damage itself but also by disrupting the systemic immune response. The aspect of immunological mechanisms in burns requires further research, and in particular, it is important to focus on younger patients as the existence of subtle differences in wound healing between adults and children may significantly influence the treatment of pediatric patients.
Prediction of Poststroke Depression Based on the Outcomes of Machine Learning Algorithms
Authors: Ryu Yeong Hwan , Kim Seo Young , Kim Tae Uk , Lee Seong Jae , Park Soo Jun , Jung Ho-Youl , Hyun Jung Keun
Published: April 2022

Poststroke depression (PSD) is a major psychiatric disorder that develops after stroke; however, whether PSD treatment improves cognitive and functional impairments is not clearly understood. We reviewed data from 31 subjects with PSD and 34 age-matched controls without PSD; all subjects underwent neurological, cognitive, and functional assessments, including the National Institutes of Health Stroke Scale (NIHSS), the Korean version of the Mini-Mental Status Examination (K-MMSE), computerized neurocognitive test (CNT), the Korean version of the Modified Barthel Index (K-MBI), and functional independence measure (FIM) at admission to the rehabilitation unit in the subacute stage following stroke and 4 weeks after initial assessments. Machine learning methods, such as support vector machine, k-nearest neighbors, random forest, voting ensemble models, and statistical analysis using logistic regression were performed. PSD was successfully predicted using a support vector machine with a radial basis function kernel function (area under curve (AUC) = 0.711, accuracy = 0.700). PSD prognoses could be predicted using a support vector machine linear algorithm (AUC = 0.830, accuracy = 0.771). The statistical method did not have a better AUC than that of machine learning algorithms. We concluded that the occurrence and prognosis of PSD in stroke patients can be predicted effectively based on patients’ cognitive and functional statuses using machine learning algorithms.
Serum Asprosin Concentrations in Children with Prader–Willi Syndrome: Correlations with Metabolic Parameters
Authors: Alsaif Maha , Field Catherine J. , Colin-Ramirez Eloisa , Prado Carla M. , Haqq Andrea M.
Published: April 2022

Children with Prader–Willi syndrome (PWS) are characterized by severe obesity. Asprosin is a newly discovered protein hormone produced by the white adipose tissue and is correlated with insulin resistance. The aim of our study was to describe the concentrations of serum asprosin in children with PWS compared to those with overweight/obesity and normal weight, and to explore the postprandial change in asprosin concentrations in participants with PWS and BMI-z matched controls. We enrolled 52 children, 23 with PWS, 8 with overweight/obesity, and 21 with normal weight. Fasting levels of asprosin, glucose, and insulin were collected in all children, and postprandial asprosin and fasting levels of acyl ghrelin (AG) and leptin were also determined in a subsample of participants. There were no significant differences among groups in fasting levels of asprosin, glucose, insulin, and HOMA-IR. Fasting serum asprosin and 1-h post-meal serum asprosin did not differ in children with PWS nor in BMI-z matched controls. Fasting asprosin showed an adjusted positive correlation with glucose in children with obesity (r = 0.93, p = 0.007) but not in children with PWS nor children with normal weight. Circulating asprosin might be a predictor of early alterations in glucose metabolism in children with obesity. More research is needed to further explain the association between asprosin, food intake, metabolism, and obesity in PWS.
Parathyroid Hormone-Related Peptide and Its Analog, Abaloparatide, Attenuate Lethal Myocardial Ischemia-Reperfusion Injury
Authors: Wider Joseph , Undyala Vishnu V. R. , Lanske Beate , Datta Nabanita S. , Przyklenk Karin
Published: April 2022

Parathyroid hormone-related peptide (PTHrP) is well-known to play a role in bone formation, and abaloparatide, an analog of PTHrP(1-34), is approved for the treatment of osteoporosis in post-menopausal women. PTHrP has also been reported to have cardiovascular effects, with recent data demonstrating that exogenously administered PTHrP can limit the death of isolated cardiomyocytes subjected to oxidative stress via upregulation of classic ‘survival kinase’ signaling. Our aim in the current study was to extend this concept and, employing both in vitro and in vivo models, establish whether PTHrP(1-36) and abaloparatide are cardioprotective in the setting of lethal myocardial ischemia-reperfusion injury. We report that preischemic administration of PTHrP(1-36) and abaloparatide attenuated cell death in HL-1 cardiomyocytes subjected to simulated ischemia-reperfusion, an effect that was accompanied by the augmented expression of phospho-ERK and improved preservation of phospho-Akt, and blocked by co-administration of the MEK-ERK inhibitor PD98059. Moreover, using the translationally relevant swine model of acute coronary artery occlusion-reperfusion, we make the novel observation that myocardial infarct size was significantly reduced in pigs pretreated with PTHrP(1-36) when compared with placebo-controls (13.1 ± 3.3% versus 42.0 ± 6.6% of the area of at-risk myocardium, respectively; p < 0.01). Taken together, these data provide the first evidence in support of the concept that pretreatment with PTHrP(1-36) and abaloparatide renders cardiomyocytes resistant to lethal myocardial ischemia-reperfusion injury.
Enhanced Electroremediation of Metals from Dredged Marine Sediment under Periodic Voltage Using EDDS and Citric Acid
Authors: Ammami Mohamed-Tahar , Benamar Ahmed , Portet-Koltalo Florence
Published: April 2022

The electrokinetic remediation (EKR) method has been extensively considered for the removal of inorganic pollutants from contaminated dredged sediment. In addition, the use of chelating agents as electrolyte solutions has been beneficial in increasing the mobility of metals. This study investigated the metals’ (Cd, Cr, Cu, Pb, and Zn) mobilities by assessing the effect of two environmentally friendly chelating agents, ethylenediaminedisuccinic acid (EDDS) and citric acid (CA), in enhancing the EKR efficiency under a periodic voltage gradient. The results showed that, for the same concentration (0.1 mol L−1), CA is more suitable for enhancing the removal of Cr (67.83%), Cu (59.77%), and Pb (32.05%) by chelating and desorbing them from the sediment matrix and concentrating them in the electrode compartments. EDDS provided efficiency to improve the Cd extraction percentage (45.87%), whereas CA and EDDS had comparable improvement removal impacts on Zn EKR (39.32% and 41.37%, respectively). From the comparison with previous results obtained with a continuous voltage, applying a periodic voltage gradient associated with a low concentration of chelating agents led to a promising result.
Spatio-Temporal Evolution and Future Simulation of Agricultural Land Use in Xiangxi, Central China
Authors: Xiang Hui , Ma Yinhua , Zhang Rongrong , Chen Hongji , Yang Qingyuan
Published: April 2022

Researches on agricultural land use would help the stakeholders to make better decisions about agricultural resources. However, studies on agricultural land have been lacking. In this context, Xiangxi was chosen as a typical region, and five indicators (Kernel Density, change importance, etc.) and two models (gray forecasting model and GeoSoS-FLUS) were used, to explore the spatio-temporal evolution trends and simulate the future scenarios of agricultural land use. The results were as follows: (1) Xiangxi was dominated by agricultural land, and nearly 50% of total extent was forestry land. Extent of agricultural land decreased by about 56.89 km2 or 3.74% from 2000 to 2018; (2) The density of each agricultural land in the study area had considerable spatial heterogeneity, and showed a main trend of shrinkage, especially in the south regions; (3) In 2030, the spatial pattern and composition of agricultural land in Xiangxi will maintain the existing status, while both of the area and proportion of agricultural land will decline, with a loss of 241.34 km2 or 2.85% decrease from 2000. Nevertheless, the study believed that the slight shrinkage of the agricultural land in Xiangxi is in line with the objective law. At the same time, the study suggested to strengthen the scientific management and rational utilization of agricultural land, with emphasis on arable land and fishery land in the south, especially the administrative center.
Mechanisms of Change in Urban Green Infrastructure—Evidence from Romania and Poland
Authors: Petrișor Alexandru-Ionuț , Mierzejewska Lidia , Mitrea Andrei
Published: April 2022

The extent and continuity of green infrastructure can be adjusted by planning. Depending on the sense of the adjustment, the process can lead to a vicious cycle, resulting in poorer urban quality, or to a virtuous planning, thus leading to psychological wellbeing and sustainability. However, socioeconomic circumstances also play an important role in managing green infrastructure. Starting from these premises, the current study aims to take an in-depth look at the mechanisms of change in urban green infrastructure and provide concrete planning recommendations for dealing with the green infrastructure. It is based on a complex approach, combining an ecological design, including geo-statistical analyses of the structure and dynamics of different categories of green infrastructure in all Romanian and Polish cities covered by the Urban Atlas data during 2006–2018, with selected case studies for analyzing the deeper mechanisms and drivers of change in green infrastructure, and focusing on the role of different planning actors. The results indicate that green infrastructure was lost in all the cities analyzed, regardless of the different planning systems of the two countries. Based on this, specific recommendations can be phrased for all stakeholders of the planning process, including planners, local administrations, policy makers, and scientists.
Assessment of MODIS Surface Temperature Products of Greenland Ice Sheet Using In-Situ Measurements
Authors: Yu Xiaoge , Wang Tingting , Ding Minghu , Wang Yetang , Sun Weijun , Zhang Qinglin , Huai Baojuan
Published: April 2022

Satellite-based data have promoted the research progress in polar regions under global climate change, meanwhile the uncertainties and limitations of satellite-derived surface temperatures are widely discussed over Greenland. This study validated the accuracy of ice surface temperature (IST) from the moderate-resolution imaging spectroradiometer (MODIS) over the Greenland ice sheet (GrIS). Daily MODIS IST was validated against the observational surface temperature from 24 automatic weather stations (AWSs) using the mean bias (MB), the root mean square (RMSE), and the correlation coefficient (R). The temporal and spatial variability over the GrIS spanning from March 2000 to December 2019 and the IST melt threshold (−1 °C) were analyzed. Generally, the MODIS IST was underestimated by an average of −2.68 °C compared to AWSs, with cold bias mainly occurring in winter. Spatially, the R and RMSE performed the better accuracy of MODIS IST on the northwest, northeast, and central part of the GrIS. Furthermore, the mean IST is mainly concentrated between −20 °C and −10 °C in summer while between −50 °C and −30 °C in winter. The largest positive IST anomalies (exceeds 3 °C) occurred in southwestern GrIS during 2010. IST shows the positive trends mainly in spring and summer and negative in autumn and winter.
Optionality in the Expression of Indefiniteness: A Pilot Study on Piacentine
Authors: Molinari Luca
Published: April 2022

Optionality is an issue within the minimalist theory of language, in which the principle of the “last resort” does not admit competing options to express the same meaning. What is needed is a solid empirical base showing that apparently competing forms do specialize for some syntactic/semantic traits. Hence, the aim of this pilot study is to investigate optionality in the choice of competing indefinite determiners in Piacentine, an Italo-Romance variety spoken in north-western Italy. Sixteen native Piacentine speakers were presented with a questionnaire to collect linguistic data. Statistical analyses of the data were performed to seek correlations between the choice of the indefinite determiners and some syntactic/semantic traits that were controlled for. The results indicate that Piacentine displays four main determiners, labeled ART, di+art, bare di, and ZERO. ART and di+art are the most widespread determiners allowing both narrow and wide scope and occurring in all the investigated contexts. Bare di is instead the unmarked form for expressing non-existential indefiniteness in negative contexts and only has narrow scope. ZERO co-varies with bare di, and its use was strengthened by contact with Italian, preventing its loss, differently from French, which lost ZERO instead.
Comorbidities in Patients with Autoimmune Bullous Disorders: Hospital-Based Registry Study
Authors: Sánchez-García Verónica , Pérez-Alcaraz Lorena , Belinchón-Romero Isabel , Ramos-Rincón Jose-Manuel
Published: April 2022

The incidence of autoimmune bullous disorders has increased over the years, especially in elderly patients with multiple comorbidities, which has stimulated research into their association with other diseases. We performed a retrospective observational study used the Minimum Basic Data Set of hospital discharges to review records of patients admitted to Spanish public hospitals between 2016 and 2019 with a diagnosis of any autoimmune bullous disorder. The objectives were to describe the comorbidity profile and the clinical-epidemiological characteristics of patients with pemphigus and pemphigoid, and analyze the evolution of the incidence of these diseases. The study included 1950 patients with pemphigus and 5424 patients with pemphigoid. Incidence increased from 2016 to 2019. The main comorbidities were hypertension (40.19%) and diabetes mellitus (28.57%). Compared to patients with pemphigoid, those with pemphigus had a higher prevalence of neoplasms, osteoporosis, solid metastases and malignant lymphoma, while the prevalence of hypertension, kidney disease, diabetes, heart failure, dementia, chronic obstructive pulmonary disease and Parkinson’s disease was higher in the pemphigoid group (p < 0.05). Therefore, since autoimmune bullous disorders are associated with diverse comorbidities and their incidence has risen in recent years, the establishment of strategies to prevent the main comorbidities in these patients is justified.
The Effect of Enterococcus faecium AL41 on the Acute Phase Proteins and Selected Mucosal Immune Molecules in Broiler Chickens
Authors: Karaffová Viera , Tóthová Csilla , Szabóová Renáta , Revajová Viera , Lauková Andrea , Ševčíková Zuzana , Herich Róbert , Žitňan Rudolf , Levkut Martin , Levkut Mikuláš , Faixová Zita , Nagy Oskar
Published: April 2022

Probiotic bacteria, including the Enterococcus faecium strain, can improve intestinal mucosal health by several mechanisms, including modulation of the immune response, as well as by improving the protective function of the epithelial barrier. In this study, we tested the effect of Enterococcus faecium AL41 on the acute phase proteins response (blood), gene expression of selected molecules of mucosal immunity (immunoglobulin A, mucin-2, insulin-like growth factor 2) and mucus production (all parts of the small intestine) in broilers. Eighty broiler chicks were divided into two groups: a control and E. faecium AL41 (birds were inoculated with AL41 for 7 days) group. The whole experiment lasted 11 days. Our results revealed that the administration of E. faecium AL41 had no substantial effect on the concentrations of acute phase proteins, but we recorded a significant increase in β- and γ-globulin fractions at the end of the experiment, which may indicate an improvement in the immune status. A significant prolonged stimulatory effect of E. faecium AL41 on the relative expression of molecules (immunoglobulin A, mucin-2) as well as on the dynamic of mucus production in the chicken intestine was observed. In addition, AL41 significantly reduced the total number of enterococci in the cecum and faeces.
Transmastoid Ultrasound Detection of Middle Ear Effusion and Its Association with Clinical Audiometric Tests
Authors: Chen Chin-Kuo , Wan Yung-Liang , Hsieh Li-Chun , Tsui Po-Hsiang
Published: April 2022

Medical history taking, otoscopy, tympanometry, and audiometry are clinical methods to diagnose middle ear effusion (MEE); however, these procedures are experience-dependent and result in misdiagnosis under unfavorable conditions of the external auditory canal in non-cooperative young children. This study aimed to explore the use of transmastoid ultrasound combined with the Nakagami parameter analysis to detect MEE in children aged 3–5 years and to compare the proposed method with clinical evaluation methods. A total of forty subjects were enrolled; for each subject, a single-element ultrasound transducer of 2.25 MHz was used to measure backscattered signals returned from the mastoid for estimating the Nakagami parameter, which is a measure of the echo amplitude distribution. Tympanogram and hearing loss were also measured for comparisons. The results showed that the Nakagami parameter in the patients with MEE was significantly larger than that of the normal group (p < 0.05). The area under the receiver operating characteristic curve (AUROC) for using the Nakagami parameter to detect MEE was 0.90, and the sensitivity, specificity, and accuracy were 82.5%, 97.5%, and 79.6%, respectively. The Nakagami parameter for tympanogram types B/C was higher than that for tympanogram type A (p < 0.05); it was also higher in the subjects with hearing loss (p < 0.05). Quantitative transmastoid ultrasound based on the Nakagami parameter analysis has the potential to detect MEE and evaluate hearing loss.
Cardiac Rehabilitation in Peripheral Artery Disease in a Tertiary Center—Impact on Arterial Stiffness and Functional Status after 6 Months
Authors: Anghel Razvan , Adam Cristina Andreea , Marcu Dragos Traian Marius , Mitu Ovidiu , Roca Mihai , Tinica Grigore , Mitu Florin
Published: April 2022

Background and Objectives: Cardiac rehabilitation (CR) plays an essential role in peripheral artery disease (PAD), leading to improved functional status, increased quality of life, and reduced arterial stiffness. We aimed to assess factors associated with clinical improvement 6 months after enrolment in a rehabilitation program at an academic medical center in north-eastern Europe. Materials and Methods: We conducted a prospective cohort study on 97 patients with PAD admitted to a single tertiary referral center. At the 6-months follow-up, 75 patients (77.3%) showed improved clinical status. We analyzed demographics and clinical and paraclinical parameters in order to explore factors associated with a favorable outcome. Results: Hypertension (p = 0.002), diabetes mellitus (p = 0.002), dyslipidemia (p = 0.045), and obesity (p = 0.564) were associated with no clinical improvement. Smoking cessation (p < 0.001), changing sedentary lifestyle (p = 0.032), and improvement of lipid and carbohydrate profile as well as functional status parameters and ambulatory arterial stiffness index (p = 0.008) were factors associated with clinical improvement at the 6-months follow-up. Conclusions: PAD patients require an integrative, multidisciplinary management to maintain functional status and increase quality of life. Improving carbohydrate and lipid profile, adopting a healthy lifestyle, quitting smoking and increasing exercise capacity are predictors for clinical improvement 6 months after enrolment in a CR program.
The Impact of Genetic Polymorphisms in Glutamate-Cysteine Ligase, a Key Enzyme of Glutathione Biosynthesis, on Ischemic Stroke Risk and Brain Infarct Size
Authors: Polonikov Alexey , Bocharova Iuliia , Azarova Iuliia , Klyosova Elena , Bykanova Marina , Bushueva Olga , Polonikova Anna , Churnosov Mikhail , Solodilova Maria
Published: April 2022

The purpose of this pilot study was to explore whether polymorphisms in genes encoding the catalytic (GCLC) and modifier (GCLM) subunits of glutamate-cysteine ligase, a rate-limiting enzyme in glutathione synthesis, play a role in the development of ischemic stroke (IS) and the extent of brain damage. A total of 1288 unrelated Russians, including 600 IS patients and 688 age- and sex-matched healthy subjects, were enrolled for the study. Nine common single nucleotide polymorphisms (SNPs) of the GCLC and GCLM genes were genotyped using the MassArray-4 system. SNP rs2301022 of GCLM was strongly associated with a decreased risk of ischemic stroke regardless of sex and age (OR = 0.39, 95%CI 0.24–0.62, p < 0.0001). Two common haplotypes of GCLM possessed protective effects against ischemic stroke risk (p < 0.01), but exclusively in nonsmoker patients. Infarct size was increased by polymorphisms rs636933 and rs761142 of GCLC. The mbmdr method enabled identifying epistatic interactions of GCLC and GCLM gene polymorphisms with known IS susceptibility genes that, along with environmental risk factors, jointly contribute to the disease risk and brain infarct size. Understanding the impact of genes and environmental factors on glutathione metabolism will allow the development of effective strategies for the treatment of ischemic stroke and disease prevention.
Prediction of Emergency Cesarean Section Using Machine Learning Methods: Development and External Validation of a Nationwide Multicenter Dataset in Republic of Korea
Authors: Wie Jeong Ha , Lee Se Jin , Choi Sae Kyung , Jo Yun Sung , Hwang Han Sung , Park Mi Hye , Kim Yeon Hee , Shin Jae Eun , Kil Ki Cheol , Kim Su Mi , Choi Bong Suk , Hong Hanul , Seol Hyun-Joo , Won Hye-Sung , Ko Hyun Sun , Na Sunghun
Published: April 2022

This study was a multicenter retrospective cohort study of term nulliparous women who underwent labor, and was conducted to develop an automated machine learning model for prediction of emergent cesarean section (CS) before onset of labor. Nine machine learning methods of logistic regression, random forest, Support Vector Machine (SVM), gradient boosting, extreme gradient boosting (XGBoost), light gradient boosting machine (LGBM), k-nearest neighbors (KNN), Voting, and Stacking were applied and compared for prediction of emergent CS during active labor. External validation was performed using a nationwide multicenter dataset for Korean fetal growth. A total of 6549 term nulliparous women was included in the analysis, and the emergent CS rate was 16.1%. The C-statistics values for KNN, Voting, XGBoost, Stacking, gradient boosting, random forest, LGBM, logistic regression, and SVM were 0.6, 0.69, 0.64, 0.59, 0.66, 0.68, 0.68, 0.7, and 0.69, respectively. The logistic regression model showed the best predictive performance with an accuracy of 0.78. The machine learning model identified nine significant variables of maternal age, height, weight at pre-pregnancy, pregnancy-associated hypertension, gestational age, and fetal sonographic findings. The C-statistic value for the logistic regression machine learning model in the external validation set (1391 term nulliparous women) was 0.69, with an overall accuracy of 0.68, a specificity of 0.83, and a sensitivity of 0.41. Machine learning algorithms with clinical and sonographic parameters at near term could be useful tools to predict individual risk of emergent CS during active labor in nulliparous women.
Dopamine and Dopamine-Related Ligands Can Bind Not Only to Dopamine Receptors
Authors: Myslivecek Jaromir
Published: April 2022

The dopaminergic system is one of the most important neurotransmitter systems in the central nervous system (CNS). It acts mainly by activation of the D1-like receptor family at the target cell. Additionally, fine-tuning of the signal is achieved via pre-synaptic modulation by the D2-like receptor family. Some dopamine drugs (both agonists and antagonists) bind in addition to DRs also to α2-ARs and 5-HT receptors. Unfortunately, these compounds are often considered subtype(s) specific. Thus, it is important to consider the presence of these receptor subtypes in specific CNS areas as the function virtually elicited by one receptor type could be an effect of other—or the co-effect of multiple receptors. However, there are enough molecules with adequate specificity. In this review, we want to give an overview of the most common off-targets for established dopamine receptor ligands. To give an overall picture, we included a discussion on subtype selectivity. Molecules used as antipsychotic drugs are reviewed too. Therefore, we will summarize reported affinities and give an outline of molecules sufficiently specific for one or more subtypes (i.e., for subfamily), the presence of DR, α2-ARs, and 5-HT receptors in CNS areas, which could help avoid ambiguous results.