bluuebunny/crossref_metadata_2025 huggingface dataset:
(+)-Usnic Acid Induces ROS-dependent Apoptosis via Inhibition of Mitochondria Respiratory Chain Complexes and Nrf2 Expression in Lung Squamous Cell Carcinoma
Authors: Qi Wanchen , Lu Changpeng , Huang Huiliang , Zhang Weinan , Song Shaofei , Liu Bing
Published: January 2020

Lung squamous cell carcinoma (LUSC) has a poor prognosis, in part due to poor therapeutic response and limited therapeutic alternatives. Lichens are symbiotic organisms, producing a variety of substances with multiple biological activities. (+)-Usnic acid, an important biologically active metabolite of lichens, has been shown to have high anti-cancer activity at low doses. However, there have been no reports regarding the effect of (+)-usnic acid on LUSC cells. This study found that (+)-usnic acid reduced viability and induced apoptosis in LUSC cells by reactive oxygen species (ROS) accumulation. (+)-Usnic acid induced mitochondria-derived ROS production via inhibition of complex I and complex III of the mitochondrial respiratory chain (MRC). Interestingly, the elimination of mitochondrial ROS by Mito-TEMPOL only partially reversed the effect of (+)-usnic acid on cellular ROS production. Further study showed that (+)-usnic acid also induced ROS production via reducing Nrf2 stability through disruption of the PI3K/Akt pathway. The in vitro and in vivo xenograft studies showed that combined treatment of (+)-usnic acid and paclitaxel synergistically suppressed LUSC cells. In conclusion, this study indicates that (+)-usnic acid induces apoptosis of LUSC cells through ROS accumulation, probably via disrupting the mitochondrial respiratory chain (MRC) and the PI3K/Akt/Nrf2 pathway. Therefore, although clinical use of (+)-usnic acid will be limited due to toxicity issues, derivatives thereof may turn out as promising anticancer candidates for adjuvant treatment of LUSC.
Measurement of 17-Hydroxyprogesterone by LCMSMS Improves Newborn Screening for CAH Due to 21-Hydroxylase Deficiency in New Zealand
Authors: de Hora Mark R. , Heather Natasha L. , Patel Tejal , Bresnahan Lauren G. , Webster Dianne , Hofman Paul L.
Published: January 2020

The positive predictive value of newborn screening for congenital adrenal hyperplasia due to 21-hydroxylase deficiency was <2% in New Zealand. This is despite a bloodspot second-tier immunoassay method for 17-hydroxyprogesterone measurement with an additional solvent extract step to reduce the number of false positive screening tests. We developed a liquid chromatography tandem mass spectrometry (LCMSMS) method to measure 17-hydroxyprogesterone in bloodspots to replace our current second-tier immunoassay method. The method was assessed using reference material and residual samples with a positive newborn screening result. Correlation with the second-tier immunoassay was determined and the method was implemented. Newborn screening performance was assessed by comparing screening metrics 2 years before and 2 years after LCMSMS implementation. Screening data analysis demonstrated the number of false positive screening tests was reduced from 172 to 40 in the 2 years after LCMSMS implementation. The positive predictive value of screening significantly increased from 1.71% to 11.1% (X2 test, p < 0.0001). LCMSMS analysis of 17OHP as a second-tier test significantly improves screening specificity for CAH due to 21-hydroxylase deficiency in New Zealand.
Still Minding the Gap? Reflecting on Transitions between Concepts of Information in Varied Domains
Authors: Bawden David , Robinson Lyn
Published: January 2020

This conceptual paper, a contribution to the tenth anniversary Special Issue of Information, gives a cross-disciplinary review of general and unified theories of information. A selective literature review is used to update a 2013 article on bridging the gaps between conceptions of information in different domains, including material from the physical and biological sciences, from the humanities and social sciences including library and information science, and from philosophy. A variety of approaches and theories are reviewed, including those of Brenner, Brier, Burgin and Wu, Capurro, Cárdenas-García and Ireland, Hidalgo, Hofkirchner, Kolchinsky and Wolpert, Floridi, Mingers and Standing, Popper, and Stonier. The gaps between disciplinary views of information remain, although there has been progress, and increasing interest, in bridging them. The solution is likely to be either a general theory of sufficient flexibility to cope with multiple meanings of information, or multiple and distinct theories for different domains, but with a complementary nature, and ideally boundary spanning concepts.
Thioester-Containing Proteins in the Drosophila melanogaster Immune Response against the Pathogen Photorhabdus
Authors: Eleftherianos Ioannis , Sachar Upasana
Published: January 2020

The fruit fly Drosophila melanogaster forms a magnificent model for interpreting conserved host innate immune signaling and functional processes in response to microbial assaults. In the broad research field of host-microbe interactions, model hosts are used in conjunction with a variety of pathogenic microorganisms to disentangle host immune system activities and microbial pathogenicity strategies. The pathogen Photorhabdus is considered an established model for analyzing bacterial virulence and symbiosis due to its unique life cycle that extends between two invertebrate hosts: an insect and a parasitic nematode. In recent years, particular focus has been given to the mechanistic participation of the D. melanogaster thioester-containing proteins (TEPs) in the overall immune capacity of the fly upon response against the pathogen Photorhabdus alone or in combination with its specific nematode vector Heterorhabditis bacteriophora. The original role of certain TEPs in the insect innate immune machinery was linked to the antibacterial and antiparasite reaction of the mosquito malaria vector Anopheles gambiae; however, revamped interest in the immune competence of these molecules has recently emerged from the D. melanogaster-Photorhabdus infection system. Here, we review the latest findings on this topic with the expectation that such information will refine our understanding of the evolutionary immune role of TEPs in host immune surveillance.
Identification of Vitis Cultivars, Rootstocks, and Species Expressing Resistance to a Planococcus Mealybug
Authors: Naegele Rachel P. , Cousins Peter , Daane Kent M.
Published: January 2020

Mealybugs cause economic loss to vineyards through physical damage, fouling fruit and leaves with honeydew, and the transmission of viruses. Planococcus ficus is one of several mealybug species in vineyards, and one that causes economic damage over a relatively large global range. To develop novel management tools, host resistance to P. ficus, which has not previously been identified for any grape cultivars, was studied. Ten grape lines (species, cultivars, and rootstocks) were evaluated for P. ficus resistance across two separate potted plant assays. Significant differences were detected among cultivars and rootstocks in the recorded number of P. ficus juveniles, adults, and egg sacs. Cabernet Sauvignon and Chardonnay were two of the most favorable grape cultivars for mealybug population growth, whereas rootstocks IAC 572, 10-17A, and RS-3 all demonstrated some level of resistance. Southern fire ant (Solenopsis xyloni) was positively associated with mealybug populations, but did not have a negative effect on the observed presence of other arthropod species including potential predators.
Assessment of Chrysoperla comanche (Banks) and Chrysoperla externa (Hagen) as Biological Control Agents of Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) on Tomato (Solanum lycopersicum) under Glasshouse Conditions
Authors: Luna-Espino Héctor Manuel , Jiménez-Pérez Alfredo , Castrejón-Gómez Víctor Rogelio
Published: January 2020

We tested the predatory capacity of newly-hatched or newly-molted Chrysoperla comanche (Banks) and Chrysoperla externa (Hagen) larvae after a 24 h fasting period on adults of Frankliniella occidentalis (Pergande) that were feeding on tomato plants (at vegetative and blooming stage) under glasshouse conditions. We also recorded fruit damage by the thrips. Both Chysoperla spp. depredated a similar number of F. occidentalis (thrips) adults regardless of the phenological stage of the plant. Second and third instar larvae of both species consumed significantly more thrips than first instar during plant blooming, however when the plant was at vegetative stage, all larval stages of both species predated a similar number of thrips. A significantly lower fruit damage percentage was recorded at the blooming plant when C. comanche larvae were in the experimental cage, however the presence of second and third instar of both species significantly reduced the fruit damage. No foliar damage was recorded. As far as we know, this is the first assessment of the predatory capacity of C. comanche and C. externa on thrips feeding on tomato under glasshouse conditions.
Prospective, Clinical Pilot Study with Eleven 4-Mm Extra-Short Implants Splinted to Longer Implants for Posterior Maxilla Rehabilitation
Authors: Torassa Daniel , Naldini Pablo , Calvo-Guirado José Luis , Fernández-Bodereau Enrique
Published: January 2020

In many clinical situations, rehabilitation with implants in the posterior maxillary region is complicated by limited bone availability. In this context, the use of 4 mm long implants (known as extra-short implants) may be used thanks to the concept of osseointegration enhancement. It has been demonstrated that short implants offer an alternative to the regeneration procedures involved in placing longer implants in areas where bone height is compromised. This prospective pilot study tested a treatment protocol in which 11 extra-short (4 mm) implants were splinted to 11 mesially placed longer (8 mm) implants in the posterior maxillary regions of partially edentulous patients, without using supplementary bone regeneration procedures. Eleven patients were included in this single cohort study. The clinical performance of the extra-short implants was assessed during a two-year follow-up period, obtaining a 100% survival rate and mean bone loss of 0.3 mm. Implant stability measured by resonance frequency analysis (RFA) at the time of placement was 54.9 ± 4.9, increasing to 77.0 ± 2.6 at 24 months. The study demonstrated the gradual consolidation of osseointegration in bone of less-than-ideal quality in the posterior maxillary region. The results obtained show that a partially edentulous maxilla with reduced bone height may be rehabilitated by using an extra-short implant splinted to a mesial implant of 8mm length or longer. Despite the small sample size, this pilot study observed that extra-short implants achieved adequate bone stability and clinical performance after a 24-month follow-up.
Comparison of the Genomic Profile of Cancer Stem Cells and Their Non-Stem Counterpart: The Case of Ovarian Cancer
Authors: Mazzoldi Elena Laura , Pastò Anna , Pilotto Giorgia , Minuzzo Sonia , Piga Ilaria , Palumbo Pietro , Carella Massimo , Frezzini Simona , Nicoletto Maria Ornella , Amadori Alberto , Indraccolo Stefano
Published: January 2020

The classical cancer stem cell (CSC) model places CSCs at the apex of a hierarchical scale, suggesting different genetic alterations in non-CSCs compared to CSCs, since an ill-defined number of cell generations and time intervals separate CSCs from the more differentiated cancer cells that form the bulk of the tumor. Another model, however, poses that CSCs should be considered a functional state of tumor cells, hence sharing the same genetic alterations. Here, we review the existing literature on the genetic landscape of CSCs in various tumor types and as a case study investigate the genomic complexity of DNA obtained from matched CSCs and non-CSCs from five ovarian cancer patients, using a genome-wide single-nucleotide polymorphism (SNP) microarray.
Metagenomic Analysis of Duodenal Microbiota Reveals a Potential Biomarker of Dysbiosis in the Course of Obesity and Type 2 Diabetes: A Pilot Study
Authors: Sroka-Oleksiak Agnieszka , Młodzińska Agata , Bulanda Małgorzata , Salamon Dominika , Major Piotr , Stanek Maciej , Gosiewski Tomasz
Published: January 2020

Numerous scientific studies confirm that, apart from environmental and genetic factors, a significant role is played by gastrointestinal microbiota in the aetiology of type 2 diabetes and obesity. Currently, scientists mainly focus on the distal intestinal microbiota, while the equally important proximal parts of the intestine are overlooked. The aim of the study was a qualitative analysis of the structure of the duodenal mucosa microbiota in groups of patients with obesity and with type 2 diabetes and where obesity qualified for bariatric surgery: sleeve gastrectomy. The microbiological results obtained were compared with some clinical parameters. As a result, it was possible to determine the microbiological core that the treatment and control groups had in common, including phyla: Firmicutes, Proteobacteria, and Actinobacteria. The patients with obesity and with type 2 diabetes and obesity presented a significantly lower number of genus Bifidobacterium compared to healthy subjects. Furthermore, the numbers of Bifidobacterium were positively correlated with the high density lipoprotein (HDL) concentration in the groups under study. The obtained results indicate that bacteria of the genus Bifidobacterium should be considered in the future in the context of a potential biomarker in the progress of type 2 diabetes and obesity.
Percutaneous Endoscopic Cervical Discectomy versus Anterior Cervical Discectomy and Fusion: A Comparative Cohort Study with a Five-Year Follow-Up
Authors: Ahn Yong , Keum Han Joong , Shin Sang Ha
Published: January 2020

Percutaneous endoscopic cervical discectomy (PECD) is an effective minimally invasive surgery for soft cervical disc herniation in properly selected cases. The current gold standard is anterior cervical discectomy and fusion (ACDF). However, few studies have evaluated the outcome of PECD compared with ACDF. We compared the surgical results of PECD and ACDF. Data from patients treated with single-level PECD (n = 51) or ACDF (n = 64) were analyzed. Patients were prospectively entered into the clinical database and their records were retrospectively reviewed. Perioperative data and clinical outcomes were evaluated using the visual analogue scale (VAS), Neck Disability Index (NDI), and modified Macnab criteria. VAS and NDI results significantly improved in both groups. The rates of excellent or good results were 88.24% and 90.63% in the PECD and ACDF group, respectively. The revision rates were 3.92% and 1.56% in the PECD and ACDF group, respectively. Operative time, hospital stay, and time to return to work were reduced in the PECD group compared to the ACDF group (p < 0.001). The five-year outcomes of PECD were comparable to those of conventional ACDF. PECD provided the typical benefits of minimally invasive surgery and may be an effective alternative for treating soft cervical disc herniation.
Massage Therapy Modulates Inflammatory Mediators Following Sprint Exercise in Healthy Male Athletes
Authors: White Gillian E. , West Sarah L. , Caterini Jessica E. , Di Battista Alex P. , Rhind Shawn G. , Wells Greg D.
Published: January 2020

Massage therapy is a common postexercise muscle recovery modality; however, its mechanisms of efficacy are uncertain. We evaluated the effects of massage on systemic inflammatory responses to exercise and postexercise muscle performance and soreness. In this crossover study, nine healthy male athletes completed a high-intensity intermittent sprint protocol, followed by massage therapy or control condition. Inflammatory markers were assessed pre-exercise; postexercise; and at 1, 2, and 24 h postexercise. Muscle performance was measured by squat and drop jump, and muscle soreness on a Likert scale. Significant time effects were observed for monocyte chemoattractant protein-1 (MCP-1), interleukin-8 (IL-8), interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor alpha (TNFα), drop jump performance, squat jump performance, and soreness. No significant effects for condition were observed. However, compared with control, inflammatory marker concentrations (IL-8, TNFα, and MCP-1) returned to baseline levels earlier following the massage therapy condition (p < 0.05 for all). IL-6 returned to baseline levels earlier following the control versus massage therapy condition (p < 0.05). No differences were observed for performance or soreness variables. MCP-1 area under the curve (AUC) was negatively associated with squat and drop jump performance, while IL-10 AUC was positively associated with drop jump performance (p < 0.05 for all). In conclusion, massage therapy promotes resolution of systemic inflammatory signaling following exercise but does not appear to improve performance or soreness measurements.
On the Relation between Friction Increase and Grease Thickener Entraining on a Border of Mixed EHL Lubrication
Authors: Zapletal Tomas , Sperka Petr , Krupka Ivan , Hartl Martin
Published: January 2020

This paper deals with an experimental study of film thickness and friction of commercial-grade grease and its base oil in a highly loaded contact. In-situ measurements were conducted for two surface textures on a ball-on-disc optical tribometer at the border of mixed lubrication. At high speeds, the film thickness and the friction of grease correspond with the base oil, while, the thickener enters the contact area and locally affects the film thickness and friction at low speeds. It was found out that the thickener starts to enter the contact area approximately at the same speed when the base oil friction increases on Stribeck curve but without direct solid to solid contact. It indicates that both effects can have the same origin. Change of lubricant flow in contact inlet area was discussed as a possible explanation.
1-Phenyl-8-[[4-(pyrrolo[1,2-a]quinoxalin-4-yl)phenyl]methyl]-1,3,8-triazaspiro[4.5]decan-4-one: Synthesis, Crystal Structure and Anti-Leukemic Activity
Authors: Guillon Jean , Savrimoutou Solène , Rubio Sandra , Moreau Stéphane , Pinaud Noël , Marchivie Mathieu , Desplat Vanessa
Published: January 2020

1-Phenyl-8-[[4-(pyrrolo[1,2-a]quinoxalin-4-yl)phenyl]methyl]-1,3,8-triazaspiro[4.5]decan-4-one has been successfully synthesized via a multi-step pathway starting from 2-nitroaniline. Structure characterization of this original pyrrolo[1,2-a]quinoxaline derivative was achieved by FT-IR, 1H-NMR, 13C-NMR, X-Ray and HRMS spectral analysis. This title compound shows interesting cytotoxic potential against several human leukemia cell lines (K562, HL60, and U937 cells).
Synthesis of Nanocrystalline Mg-Al Hydrotalcites in the Presence of Starch—the Effect on Structure and Composition
Authors: Michalik Alicja , Napruszewska Bogna D. , Walczyk Anna , Kryściak-Czerwenka Joanna , Duraczyńska Dorota , Serwicka Ewa M.
Published: January 2020

The study describes the synthesis of Mg-Al hydrotalcite (Ht) with the use of starch as a structure controlling biotemplate. Syntheses were carried out at room temperature, by co-precipitation at pH = 10. The investigated synthesis parameters included the nature of the precipitating agent (NaOH/Na2CO3 or NH3aq/(NH4)2CO3), the nature of starch (potato, corn and cassava), the method of starch addition to reagents, the method of drying and the effect of washing. The materials were examined with X-ray diffraction, scanning electron microscopy/energy dispersive X-ray spectroscopy and infrared spectroscopy. The data show that synthesis of Ht materials in the presence of starch, with use of the ammonia-based precipitant, enabled preparation of nanocrystalline Ht with very fine (<50 nm) particle size. All investigated starches had a similar effect on the crystallinity and the grain size of Ht precipitates. Ht with the smallest nanocrystals was obtained when starch was present in all solutions used for synthesis, and the final product subjected to freeze drying. Washing with water was found to enhance recrystallization and exchange of nitrates for carbonates. Infrared spectra showed that an interaction exists between the biopolymer template and the Ht particles, resulting in a higher degree of order within the Ht-adhering starch component.
Sustainable Test Methods for Construction Materials and Elements
Authors: Szewczak Ewa , Winkler-Skalna Agnieszka , Czarnecki Lech
Published: January 2020

The laboratory testing of the construction materials and elements is a subset of activities inherent in sustainable building materials engineering. Two questions arise regarding test methods used: the relation between test results and material behavior in actual conditions on the one hand, and the variability of results related to uncertainty on the other. The paper presents the analysis of the results and uncertainties of the simple two independent test examples (bond strength and tensile strength) in order to demonstrate discrepancies related to the ambiguous methods of estimating uncertainty and the consequences of using test methods when method suitability for conformity assessment has not been properly verified. Examples are the basis for opening discussion on the test methods development direction, which makes possible to consider them as ‘sustainable’. The authors address the negative impact of the lack of a complete test models taking into account proceeding with an uncertainty on erroneous assessment risks. Adverse effects can be minimized by creating test methods appropriate for the test’s purpose (e.g., initial or routine tests) and handling with uncontrolled uncertainty components. Sustainable test methods should ensure a balance between widely defined tests and evaluation costs and the material’s or building’s safety, reliability, and stability.
Computational Techniques in Numerical Simulations of Arc and Laser Welding Processes
Authors: Kik Tomasz
Published: January 2020

The article presents a comparison of modern computational techniques used in numerical analyses of welding processes. The principles of the “transient” technique calculations with a moving heat source, the “macro-bead” (MBD) technique, with an imposed thermal cycle on a selected weld bead section and the “local–global” approach with shrinkage calculation technique were described. They can be used, depending on the variant chosen, both for individual, simple weld joints and those made of many beads or constructions containing dozens of welds and welded elements. Differences in the obtained results and time needed to perform calculations with four different calculation examples of single and multipass arc and laser beam welding processes were presented. The results of calculations of displacements and stresses distributions in the welded joints using various computational techniques were compared, as well as the calculation times with the described techniques. The numerical analyses in the SYSWELD software package have shown the differences between the described computational techniques, as well as an understanding of the benefits and disadvantages of using each of them. This knowledge allows preparing an efficient and fast optimization of the welding processes, often aimed at minimizing deformations in the first place, as well as detection of potential defects of both simple and complex welded structures. In general, the possibilities and flexibility of modern numerical calculation software have been presented.
Reduced-Order Model of Rotor Cage in Multiphase Induction Machines: Application on the Prediction of Torque Pulsations
Authors: Mekahlia Abdelhak , Semail Eric , Scuiller Franck , Zahr Hussein
Published: January 2020

For three-phase induction machines supplied by sinusoidal current, it is usual to model the n-bar squirrel-cage by an equivalent two-phase circuit. For a multiphase induction machine which can be supplied with different harmonics of current, the reduced-order model of the rotor must be more carefully chosen in order to predict the pulsations of torque. The proposed analysis allows to avoid a wrong design with non-sinusoidal magnetomotive forces. An analytical approach is proposed and confirmed by Finite-Element modelling at first for a three-phase induction machine and secondly for a five-phase induction machine.
The Marine Seagrass Halophila stipulacea as a Source of Bioactive Metabolites against Obesity and Biofouling
Authors: Bel Mabrouk Sawssen , Reis Mariana , Sousa Maria Lígia , Ribeiro Tiago , Almeida Joana R. , Pereira Sandra , Antunes Jorge , Rosa Filipa , Vasconcelos Vitor , Achour Lotfi , Kacem Adnen , Urbatzka Ralph
Published: January 2020

Marine organisms, including seagrasses, are important sources of biologically active molecules for the treatment of human diseases. In this study, organic extracts of the marine seagrass Halophila stipulacea obtained by different polarities from leaves (L) and stems (S) (hexane [HL, HS], ethyl acetate [EL, ES], and methanol [ML, MS]) were tested for different bioactivities. The screening comprehended the cytotoxicity activity against cancer cell lines grown as a monolayer culture or as multicellular spheroids (cancer), glucose uptake in cells (diabetes), reduction of lipid content in fatty acid-overloaded liver cells (steatosis), and lipid-reducing activity in zebrafish larvae (obesity), as well as the antifouling activity against marine bacteria (microfouling) and mussel larval settlement (macrofouling). HL, EL, HS, and ES extracts showed statistically significant cytotoxicity against cancer cell lines. The extracts did not have any significant effect on glucose uptake and on the reduction of lipids in liver cells. The EL and ML extracts reduced neutral lipid contents on the larvae of zebrafish with EC50 values of 2.2 µg/mL for EL and 1.2 µg/mL for ML. For the antifouling activity, the HS and ML extracts showed a significant inhibitory effect (p < 0.05) against the settlement of Mytilus galloprovincialis plantigrade larvae. The metabolite profiling using HR-LC-MS/MS and GNPS (The Global Natural Product Social Molecular Networking) analyses identified a variety of known primary and secondary metabolites in the extracts, along with some unreported molecules. Various compounds were detected with known activities on cancer (polyphenols: Luteolin, apeginin, matairesinol), on metabolic diseases (polyphenols: cirsimarin, spiraeoside, 2,4-dihydroxyheptadec-16-ynyl acetate; amino acids: N-acetyl-L-tyrosine), or on antifouling (fatty acids: 13-decosenamide; cinnamic acids: 3-hydroxy-4-methoxycinnamic acid, alpha-cyano-4-hydroxycinnamic), which could be, in part, responsible for the observed bioactivities. In summary, this study revealed that Halophila stipulacea is a rich source of metabolites with promising activities against obesity and biofouling and suggests that this seagrass could be useful for drug discovery in the future.
Financial Burden of Stroke Reflected in a Pilot Center for the Implementation of Thrombolysis
Authors: Uivarosan Diana , Bungau Simona , Tit Delia Mirela , Moisa Corina , Fratila Ovidiu , Rus Marius , Bratu Ovidiu Gabriel , Diaconu Camelia C. , Pantis Carmen
Published: January 2020

Stroke represents a serious illness and is extremely relevant from the public health point of view, implying important social and economic burdens. Introducing new procedures or therapies that reduce the costs both in the acute phase of the disease and in the long term becomes a priority for health systems worldwide. The present study quantifies and compares the direct costs for ischemic stroke in patients with thrombolysis treatment versus conservative treatment over a 24-month period from the initial diagnosis, in one of the 7 national pilot centres for the implementation of thrombolytic treatment. The significant reduction (p < 0.001) of the hospitalization period, especially of the days in the intensive care unit (ICU) for stroke, resulted in a significant reduction (p < 0.001) of the total average costs in the patients with thrombolysis, both at the first hospitalization and for the subsequent hospitalizations, during the period followed in the study. It was also found that the percentage of patients who were re-hospitalized within the first 24-months after stroke was significantly lower (p < 0.001) among thrombolyzed patients. The present study demonstrates that the quick intervention in cases of stroke is an efficient policy regarding costs, of Romanian Public Health System, Romania being the country with the highest rates of new strokes and deaths due to stroke in Europe.
Antibacterial Properties of Nanoparticles in Dental Restorative Materials. A Systematic Review and Meta-Analysis
Authors: Ferrando-Magraner Elena , Bellot-Arcís Carlos , Paredes-Gallardo Vanessa , Almerich-Silla José Manuel , García-Sanz Verónica , Fernández-Alonso Mercedes , Montiel-Company José María
Published: January 2020

Background and Objectives: Nanotechnology has become a significant area of research focused mainly on increasing the antibacterial and mechanical properties of dental materials. The aim of the present systematic review and meta-analysis was to examine and quantitatively analyze the current evidence for the addition of different nanoparticles into dental restorative materials, to determine whether their incorporation increases the antibacterial/antimicrobial properties of the materials. Materials and Methods: A literature search was performed in the Pubmed, Scopus, and Embase databases, up to December 2018, following PRISMA (Preferred Reporting Items for Systematic Review and Meta-Analysis) guidelines for systematic reviews and meta-analyses. Results: A total of 624 papers were identified in the initial search. After screening the texts and applying inclusion criteria, only 11 of these were selected for quantitative analysis. The incorporation of nanoparticles led to a significant increase (p-value < 0.01) in the antibacterial capacity of all the dental materials synthesized in comparison with control materials. Conclusions: The incorporation of nanoparticles into dental restorative materials was a favorable option; the antibacterial activity of nanoparticle-modified dental materials was significantly higher compared with the original unmodified materials, TiO2 nanoparticles providing the greatest benefits. However, the high heterogeneity among the articles reviewed points to the need for further research and the application of standardized research protocols.
Toward a Standardized Strategy of Clinical Metabolomics for the Advancement of Precision Medicine
Authors: Long Nguyen Phuoc , Nghi Tran Diem , Kang Yun Pyo , Anh Nguyen Hoang , Kim Hyung Min , Park Sang Ki , Kwon Sung Won
Published: January 2020

Despite the tremendous success, pitfalls have been observed in every step of a clinical metabolomics workflow, which impedes the internal validity of the study. Furthermore, the demand for logistics, instrumentations, and computational resources for metabolic phenotyping studies has far exceeded our expectations. In this conceptual review, we will cover inclusive barriers of a metabolomics-based clinical study and suggest potential solutions in the hope of enhancing study robustness, usability, and transferability. The importance of quality assurance and quality control procedures is discussed, followed by a practical rule containing five phases, including two additional “pre-pre-” and “post-post-” analytical steps. Besides, we will elucidate the potential involvement of machine learning and demonstrate that the need for automated data mining algorithms to improve the quality of future research is undeniable. Consequently, we propose a comprehensive metabolomics framework, along with an appropriate checklist refined from current guidelines and our previously published assessment, in the attempt to accurately translate achievements in metabolomics into clinical and epidemiological research. Furthermore, the integration of multifaceted multi-omics approaches with metabolomics as the pillar member is in urgent need. When combining with other social or nutritional factors, we can gather complete omics profiles for a particular disease. Our discussion reflects the current obstacles and potential solutions toward the progressing trend of utilizing metabolomics in clinical research to create the next-generation healthcare system.
Fabrication and Characterization of Humidity Sensors Based on Graphene Oxide–PEDOT:PSS Composites on a Flexible Substrate
Authors: Romero Francisco J. , Rivadeneyra Almudena , Becherer Markus , Morales Diego P. , Rodríguez Noel
Published: January 2020

In this paper, we present a simple, fast, and cost-effective method for the large-scale fabrication of high-sensitivity humidity sensors on flexible substrates. These sensors consist of a micro screen-printed capacitive structure upon which a sensitive layer is deposited. We studied two different structures and three different sensing materials by modifying the concentration of poly(3,4-ethylenedioxythiophene)/polystyrene sulfonate (PEDOT:PSS) in a graphene oxide (GO) solution. The results show that the aggregation of the PEDOT:PSS to the GO can modify its electrical properties, boosting the performance of the capacitive sensors in terms of both resistive losses and sensitivity to relative humidity (RH) changes. Thus, in an area less than 30 mm2, the GO/PEDOT:PSS-based sensors can achieve a sensitivity much higher (1.22 nF/%RH at 1 kHz) than other similar sensors presented in the literature which, together with their good thermal stability, time response, and performance over bending, demonstrates that the manufacturing approach described in this work paves the way for the mass production of flexible humidity sensors in an inexpensive way.
Centrifugal Microfluidics Traps for Parallel Isolation and Imaging of Single Cells
Authors: Snider Adam , Pirozzi Ileana , Tripathi Anubhav
Published: January 2020

Analysis at the single cell level has becoming an increasingly important procedure to diagnose cancer tissue biopsies. These tissue samples are often heterogeneous and consist of 1000–15,000 cells. We study the use of centrifugal microfluidics to isolate single cells into micro chambers. We describe the optimization of our microfluidics flow device, characterize its performance using both polystyrene beads as a cell analogue and MCF-7 breast cancer cells, and discuss potential applications for the device. Our results show rapid isolation of ~2000 single cell aliquots in ~20 min. We were able to occupy 65% of available chambers with singly occupied cancer cells, and observed capture efficiencies as high as 80% using input samples ranging from 2000 to 15,000 cells in 20 min. We believe our device is a valuable research tool that addresses the unmet need for massively parallel single cell level analysis of cell populations.
Model-Driven Controlled Alteration of Nanopillar Cap Architecture Reveals its Effects on Bactericidal Activity
Authors: Zahir Taiyeb , Pesek Jiri , Franke Sabine , Van Pee Jasper , Rathore Ashish , Smeets Bart , Ramon Herman , Xu Xiumei , Fauvart Maarten , Michiels Jan
Published: January 2020

Nanostructured surfaces can be engineered to kill bacteria in a contact-dependent manner. The study of bacterial interactions with a nanoscale topology is thus crucial to developing antibacterial surfaces. Here, a systematic study of the effects of nanoscale topology on bactericidal activity is presented. We describe the antibacterial properties of highly ordered and uniformly arrayed cotton swab-shaped (or mushroom-shaped) nanopillars. These nanostructured surfaces show bactericidal activity against Staphylococcus aureus and Pseudomonas aeruginosa. A biophysical model of the cell envelope in contact with the surface, developed ab initio from the infinitesimal strain theory, suggests that bacterial adhesion and subsequent lysis are highly influenced by the bending rigidity of the cell envelope and the surface topography formed by the nanopillars. We used the biophysical model to analyse the influence of the nanopillar cap geometry on the bactericidal activity and made several geometrical alterations of the nanostructured surface. Measurement of the bactericidal activities of these surfaces confirms model predictions, highlights the non-trivial role of cell envelope bending rigidity, and sheds light on the effects of nanopillar cap architecture on the interactions with the bacterial envelope. More importantly, our results show that the surface nanotopology can be rationally designed to enhance the bactericidal efficiency.
Insights into the CaSO4–H2O System: A Raman-Spectroscopic Study
Authors: Schmid Thomas , Jungnickel Robert , Dariz Petra
Published: January 2020

Even though being the subject of natural scientific research for many decades, the system CaSO4–H2O, consisting of the five crystalline phases gypsum, bassanite, and the anhydrites III, II, and I, has left many open questions for research. Raman spectroscopy was used because of its structural sensitivity and in situ measurement capability to obtain further insight by studying phase transitions in both ex situ and in situ experiments. The findings include significant contributions to the completeness and understanding of Raman spectroscopic data of the system. The dehydration path gypsum–bassanite–anhydrite III was shown to have strong parallels to a physical drying process, which depends on many parameters beyond the burning temperature. Raman band width determination was demonstrated to enable the quantitative discrimination of α-bassanite and β-bassanite as well as the postulated three sub-forms of anhydrite II (AII), which are all based on differences in crystallinity. In the latter case, the observed continuous structural variations over increasing burning temperatures were elucidated as a combination of decreasing surface areas and healing of crystal lattice defects. We propose an only two-fold sub-division of AII into reactive “disordered AII” and much less reactive “crystalline AII” with a transition temperature of 650 °C ± 50 K.
The Performance and Adsorption Mechanism of a Novel Collector, Dodecyl Dimethyl Betaine (BS-12), for the Flotation Separation of Ilmenite and Titanaugite
Authors: Chen Pan , Chai Xujian , Tian Mengjie , Chen Wei , Wan Si , Meng Qingbo , Zhai Jihua , Gao Yude
Published: January 2020

In this paper, a novel collector, dodecyl dimethyl betaine (BS-12), was used in the selective separation of ilmenite from titanaugite. The flotation performance and associated adsorption mechanism were studied by micro-flotation experiments, particle size analysis, Fourier-transform infrared (FT-IR) spectroscopy analysis, and X-ray photoelectron spectroscopy (XPS) analysis. The micro-flotation results indicated that BS-12 exhibited a stronger collecting ability towards ilmenite than titanaugite within an acidic pH range, and that the recovery of ilmenite was about 50% higher than that of titanaugite under the optimum flotation conditions. Particle size analysis demonstrated that BS-12 could selectively agglomerate ilmenite to a certain extent and then contribute to the flotation difference between ilmenite and titanaugite. FT-IR results showed some characteristic bands of BS-12 on treated ilmenite, and on titanaugite with BS-12. The XPS analysis further confirmed that BS-12 chemisorbed onto ilmenite and titanaugite in a similar way, but the limited active sites on titanaugite in comparison with ilmenite accounted for their differences in flotation.
Mineralogical Characterization of Dolomitic Aggregate Concrete: The Camarasa Dam (Catalonia, Spain)
Authors: Garcia Encarnación , Alfonso Pura , Tauler Esperança
Published: January 2020

The Camarasa Dam was built in 1920 using dolomitic aggregate and Portland cement with two different compositions: type A (dolomite and Portland cement) and type B (dolomite and sand-cement). The sand cement was a finely powdered mixture of dolomite particles and clinker of Portland cement. The mineralogy of concrete was studied by optical microscopy, scanning electron microscopy, and x-ray powder diffraction. Reaction of dedolomitization occurred in the two types of concrete of the Camarasa Dam, as demonstrated by the occurrence of calcite, brucite, and/or absence of portlandite. In the type A concrete, calcite, brucite, and a serpentine-group mineral precipitated as a rim around the dolomite grains and in the paste. The rims, a product of the dedolomitization reaction, protected the surface of dolomite from the dissolution process. In type B concrete, in addition to dolomite and calcite, quartz and K-feldspar were present. Brucite occurred in lower amounts than in the type A concrete as fibrous crystals randomly distributed in the sand-cement paste. Although brucite content was higher in the type A concrete, type B showed more signs of loss of durability. This can be attributed to the further development of the alkali-silica reaction in this concrete type.
Few Layer Graphene Does Not Affect Cellular Homeostasis of Mouse Macrophages
Authors: Malanagahalli Sowmya , Murera Diane , Martín Cristina , Lin Hazel , Wadier Nadége , Dumortier Hélène , Vázquez Ester , Bianco Alberto
Published: January 2020

Graphene-related materials (GRMs) are widely used in various applications due to their unique properties. A growing number of reports describe the impact of different carbon nanomaterials, including graphene oxide (GO), reduced GO (rGO), and carbon nanotubes (CNT), on immune cells, but there is still a very limited number of studies on graphene. In this work, we investigated the biological responses of few layer graphene (FLG) on mouse macrophages (bone marrow derived macrophages, BMDMs), which are part of the first line of defense in innate immunity. In particular, our paper describes our findings of short-term FLG treatment in BMDMs with a focus on observing material internalization and changes in general cell morphology. Subsequent investigation of cytotoxicity parameters showed that increasing doses of FLG did not hamper the viability of cells and did not trigger inflammatory responses. Basal level induced autophagic activity sufficed to maintain the cellular homeostasis of FLG treated cells. Our results shed light on the impact of FLG on primary macrophages and show that FLG does not elicit immunological responses leading to cell death.
Graphene-Coated Nanowire Waveguides and Their Applications
Authors: Teng Da , Wang Kai , Li Zhe
Published: January 2020

In recent years, graphene-coated nanowires (GCNWs) have attracted considerable research interest due to the unprecedented optical properties of graphene in terahertz (THz) and mid-infrared bands. Graphene plasmons in GCNWs have become an attractive platform for nanoscale applications in subwavelength waveguides, polarizers, modulators, nonlinear devices, etc. Here, we provide a comprehensive overview of the surface conductivity of graphene, GCNW-based plasmon waveguides, and applications of GCNWs in optical devices, nonlinear optics, and other intriguing fields. In terms of nonlinear optical properties, the focus is on saturable absorption. We also discuss some limitations of the GCNWs. It is believed that the research of GCNWs in the field of nanophotonics will continue to deepen, thus laying a solid foundation for its practical application.
Cerium Oxide Nanoparticles Rescue α-Synuclein-Induced Toxicity in a Yeast Model of Parkinson’s Disease
Authors: Ruotolo Roberta , De Giorgio Giuseppe , Minato Ilaria , Bianchi Massimiliano , Bussolati Ovidio , Marmiroli Nelson
Published: January 2020

Over the last decades, cerium oxide nanoparticles (CeO2 NPs) have gained great interest due to their potential applications, mainly in the fields of agriculture and biomedicine. Promising effects of CeO2 NPs are recently shown in some neurodegenerative diseases, but the mechanism of action of these NPs in Parkinson’s disease (PD) remains to be investigated. This issue is addressed in the present study by using a yeast model based on the heterologous expression of the human α-synuclein (α-syn), the major component of Lewy bodies, which represent a neuropathological hallmark of PD. We observed that CeO2 NPs strongly reduce α-syn-induced toxicity in a dose-dependent manner. This effect is associated with the inhibition of cytoplasmic α-syn foci accumulation, resulting in plasma membrane localization of α-syn after NP treatment. Moreover, CeO2 NPs counteract the α-syn-induced mitochondrial dysfunction and decrease reactive oxygen species (ROS) production in yeast cells. In vitro binding assay using cell lysates showed that α-syn is adsorbed on the surface of CeO2 NPs, suggesting that these NPs may act as a strong inhibitor of α-syn toxicity not only acting as a radical scavenger, but through a direct interaction with α-syn in vivo.
Protein Corona Composition of Silica Nanoparticles in Complex Media: Nanoparticle Size does not Matter
Authors: Marichal Laurent , Klein Géraldine , Armengaud Jean , Boulard Yves , Chédin Stéphane , Labarre Jean , Pin Serge , Renault Jean-Philippe , Aude Jean-Christophe
Published: January 2020

Biomolecules, and particularly proteins, bind on nanoparticle (NP) surfaces to form the so-called protein corona. It is accepted that the corona drives the biological distribution and toxicity of NPs. Here, the corona composition and structure were studied using silica nanoparticles (SiNPs) of different sizes interacting with soluble yeast protein extracts. Adsorption isotherms showed that the amount of adsorbed proteins varied greatly upon NP size with large NPs having more adsorbed proteins per surface unit. The protein corona composition was studied using a large-scale label-free proteomic approach, combined with statistical and regression analyses. Most of the proteins adsorbed on the NPs were the same, regardless of the size of the NPs. To go beyond, the protein physicochemical parameters relevant for the adsorption were studied: electrostatic interactions and disordered regions are the main driving forces for the adsorption on SiNPs but polypeptide sequence length seems to be an important factor as well. This article demonstrates that curvature effects exhibited using model proteins are not determining factors for the corona composition on SiNPs, when dealing with complex biological media.
Irritable Bowel Syndrome-Like Disorders in Endometriosis: Prevalence of Nickel Sensitivity and Effects of a Low-Nickel Diet. An Open-Label Pilot Study
Authors: Borghini Raffaele , Porpora Maria Grazia , Casale Rossella , Marino Mariacatia , Palmieri Emilia , Greco Nicoletta , Donato Giuseppe , Picarelli Antonio
Published: January 2020

Alimentary nickel (Ni) may result in allergic contact mucositis (ACM), whose prevalence is >30% and may present with IBS-like and extra-intestinal symptoms. These symptoms are also frequent in endometriosis, and Ni allergic contact dermatitis has already been observed in endometriosis. Therefore, intestinal and extra-intestinal symptoms in endometriosis may depend on a Ni ACM, and a low-Ni diet could improve symptoms. We studied the prevalence of Ni ACM in endometriosis and focused on the effects of a low-Ni diet on gastrointestinal, extra-intestinal, and gynecological symptoms. We recruited 84 women with endometriosis, symptomatic for gastrointestinal disorders. Thirty-one out of 84 patients completed the study. They underwent Ni oral mucosa patch test (omPT), questionnaire for intestinal/extra-intestinal/gynecological symptoms, and a low-Ni diet. Clinical evaluation was performed at baseline (T0) and after three months (T1). Twenty-eight out 31 (90.3%) patients showed Ni omPT positive results, with Ni ACM diagnosis, whereas three out of 31 (9.7%) patients showed negative Ni omPT. After three months of low-Ni diet, all gastrointestinal, extra-intestinal and gynecological symptoms showed a statistically significant reduction. Ni ACM has a high prevalence in endometriosis and a low-Ni diet may be recommended in this condition to reduce gastrointestinal, extra-intestinal and gynecological symptoms.