bluuebunny/crossref_metadata_2025 huggingface dataset:
Based on cuproptosis-related lncRNAs, a novel prognostic signature for colon adenocarcinoma prognosis, immunotherapy, and chemotherapy response
Authors: Li Chong , Zhang Keqian , Gong Yuzhu , Wu Qinan , Zhang Yanyan , Dong Yan , Li Dejia , Wang Zhe
Published: June 2023

Introduction: Colon adenocarcinoma (COAD) is a special pathological subtype of colorectal cancer (CRC) with highly heterogeneous solid tumors with poor prognosis, and novel biomarkers are urgently required to guide its prognosis.

Material and methods: RNA-Seq data of COAD were downloaded through The Cancer Genome Atlas (TCGA) database to determine cuproptosis-related lncRNAs (CRLs) using weighted gene co-expression network analysis (WGCNA). The scores of the pathways were calculated by single-sample gene set enrichment analysis (ssGSEA). CRLs that affected prognoses were determined via the univariate COX regression analysis to develop a prognostic model using multivariate COX regression analysis and LASSO regression analysis. The model was assessed by applying Kaplan–Meier (K-M) survival analysis and receiver operating characteristic curves and validated in GSE39582 and GSE17538. The tumor microenvironment (TME), single nucleotide variants (SNV), and immunotherapy response/chemotherapy sensitivity were assessed in high- and low-score subgroups. Finally, the construction of a nomogram was adopted to predict survival rates of COAD patients during years 1, 3, and 5.

Results: We found that a high cuproptosis score reduced the survival rates of COAD significantly. A total of five CRLs affecting prognosis were identified, containing AC008494.3, EIF3J-DT, AC016027.1, AL731533.2, and ZEB1-AS1. The ROC curve showed that RiskScore could perform well in predicting the prognosis of COAD. Meanwhile, we found that RiskScore showed good ability in assessing immunotherapy and chemotherapy sensitivity. Finally, the nomogram and decision curves showed that RiskScore would be a powerful predictor for COAD.

Conclusion: A novel prognostic model was constructed using CRLs in COAD, and the CRLs in the model were probably a potential therapeutic target. Based on this study, RiskScore was an independent predictor factor, immunotherapy response, and chemotherapy sensitivity for COAD, providing a new scientific basis for COAD prognosis management.
“Biofeedback-based return to sport”: individualization through objective assessments
Authors: Ekizos Antonis , Santuz Alessandro
Published: June 2023

Elite athletes are regularly exposed to high and repetitive mechanical stresses and impacts, resulting in high injury rates. The consequences of injury can range from time lost from training and competition to chronic physical and psychological burden, with no guarantee that the athlete will return to preinjury levels of sport activity and performance. Prominent predictors include load management and previous injury, highlighting the importance of the postinjury period for effective return to sport (RTS). Currently, there is conflicting information on how to choose and assess the best reentry strategy. Treating RTS as a continuum, with controlled progression of training load and complexity, seems to provide benefits in this process. Furthermore, objectivity has been identified as a critical factor in improving the effectiveness of RTS. We propose that assessments derived from biomechanical measurements in functional settings can provide the objectivity needed for regular biofeedback cycles. These cycles should aim to identify weaknesses, customize the load, and inform on the status of RTS progress. This approach emphasizes individualization as the primary determinant of RTS and provides a solid foundation for achieving it.
3D data-augmentation methods for semantic segmentation of tomato plant parts
Authors: Xin Bolai , Sun Ji , Bartholomeus Harm , Kootstra Gert
Published: June 2023

Introduction

3D semantic segmentation of plant point clouds is an important step towards automatic plant phenotyping and crop modeling. Since traditional hand-designed methods for point-cloud processing face challenges in generalisation, current methods are based on deep neural network that learn to perform the 3D segmentation based on training data. However, these methods require a large annotated training set to perform well. Especially for 3D semantic segmentation, the collection of training data is highly labour intensitive and time consuming. Data augmentation has been shown to improve training on small training sets. However, it is unclear which data-augmentation methods are effective for 3D plant-part segmentation.

Methods

In the proposed work, five novel data-augmentation methods (global cropping, brightness adjustment, leaf translation, leaf rotation, and leaf crossover) were proposed and compared to five existing methods (online down sampling, global jittering, global scaling, global rotation, and global translation). The methods were applied to PointNet++ for 3D semantic segmentation of the point clouds of three cultivars of tomato plants (Merlice, Brioso, and Gardener Delight). The point clouds were segmented into soil base, stick, stemwork, and other bio-structures.

Results and disccusion

Among the data augmentation methods being proposed in this paper, leaf crossover indicated the most promising result which outperformed the existing ones. Leaf rotation (around Z axis), leaf translation, and cropping also performed well on the 3D tomato plant point clouds, which outperformed most of the existing work apart from global jittering. The proposed 3D data augmentation approaches significantly improve the overfitting caused by the limited training data. The improved plant-part segmentation further enables a more accurate reconstruction of the plant architecture.
Transcriptomic and metabolomic analyses reveal the potential mechanism of waterlogging resistance in cotton (Gossypium hirsutum L.)
Authors: Owusu Anane Gideon , Lv Yin-Ping , Liu Man , Wu Yong , Li Cheng-Lin , Guo Ning , Li Da-Hui , Gao Jun-Shan
Published: June 2023

Introduction

Cotton (Gossypium hirsutum L.) is susceptible to long-term waterlogging stress; however, genomic information of cotton response mechanisms toward long days of waterlogging is quite elusive.

Methods

Here, we combined the transcriptome and metabolome expression level changes in cotton roots after 10 and 20 days of waterlogging stress treatment pertaining to potential resistance mechanisms in two cotton genotypes.

Results and discussion

Numerous adventitious roots and hypertrophic lenticels were induced in CJ1831056 and CJ1831072. Transcriptome analysis revealed 101,599 differentially expressed genes in cotton roots with higher gene expression after 20 days of stress. Reactive oxygen species (ROS) generating genes, antioxidant enzyme genes, and transcription factor genes (AP2, MYB, WRKY, and bZIP) were highly responsive to waterlogging stress among the two genotypes. Metabolomics results showed higher expressions of stress-resistant metabolites sinapyl alcohol, L-glutamic acid, galactaric acid, glucose 1-phosphate, L-valine, L-asparagine, and melibiose in CJ1831056 than CJ1831072. Differentially expressed metabolites (adenosine, galactaric acid, sinapyl alcohol, L-valine, L-asparagine, and melibiose) significantly correlated with the differentially expressed PRX52, PER1, PER64, and BGLU11 transcripts. This investigation reveals genes for targeted genetic engineering to improve waterlogging stress resistance to enhance abiotic stress regulatory mechanisms in cotton at the transcript and metabolic levels of study.
Genome-wide association analysis for emergence of deeply sown rice (Oryza sativa) reveals novel aus-specific phytohormone candidate genes for adaptation to dry-direct seeding in the field
Authors: Sakhale Sandeep A. , Yadav Shailesh , Clark Lindsay V. , Lipka Alexander E. , Kumar Arvind , Sacks Erik J.
Published: June 2023

Dry direct-seeded rice (dry-DSR) is typically sown deeply to circumvent the need for irrigation, and thus seedling emergence is a crucial trait affecting plant stand and yield. To breed elite cultivars that use less water and are climate-resilient, an understanding of the genomic regions and underlying genes that confer emergence in deeply sown dry-DSR would be highly advantageous. A combined diversity panel of 470 rice accessions (RDP1 plus aus subset of 3K RGP) was evaluated with 2.9 million single nucleotide polymorphisms (SNPs) to identify associations with dry-DSR traits in the field and component traits in a controlled-environment experiment. Using genome-wide association study (GWAS) analyses, we identified 18 unique QTLs on chromosomes 1, 2, 4, 5, 6, 7, 9, 10, and 11, explaining phenotypic variance ranging from 2.6% to 17.8%. Three QTLs, namely,qSOE-1.1,qEMERG-AUS-1.2, andqEMERG-AUS-7.1, were co-located with previously reported QTLs for mesocotyl length. Among the identified QTLs, half were associated with the emergence of aus, and six were unique to the aus genetic group. Based on functional annotation, we identified eleven compelling candidate genes that primarily regulate phytohormone pathways such as cytokinin, auxin, gibberellic acid, and jasmonic acid. Prior studies indicated that these phytohormones play a critical role in mesocotyl length under deep sowing. This study provides new insight into the importance of aus andindicaas desirable genetic resources to mine favorable alleles for deep-sowing tolerance in rice. The candidate genes and marker-tagged desirable alleles identified in this study should benefit rice breeding programs directly.
Effectiveness of exercise intervention on improving fundamental motor skills in children with autism spectrum disorder: a systematic review and meta-analysis
Authors: Ji Yu-Qin , Tian Hao , Zheng Ze-Yu , Ye Zhuo-Yan , Ye Qiang
Published: June 2023

Background

Autism spectrum disorder (ASD) is a severe public health concern, and most of the children with ASD experience a substantial delay in FMS. This study aimed to investigate the effectiveness of exercise interventions in improving FMS in children with ASD, and provide evidence to support the scientific use of exercise interventions in practice.

Methods

We searched seven online databases (PubMed, Scopus, Web of Science, Embase, EBSCO, Clinical Trials, and The Cochrane Library) from inception to May 20, 2022. We included randomized control trials of exercise interventions for FMS in children with ASD. The methodological quality of the included studies was assessed using the Physiotherapy Evidence Database Scale. Stata 14.0 software was used for meta-analysis, forest plotting, subgroup analysis, heterogeneity analysis, and meta-regression.

Results

Thirteen studies underwent systematic review (541 participants), of which 10 underwent meta-analysis (297 participants). Overall, exercise interventions significantly improved overall FMS in children with ASD. Regarding the three categories of FMS, exercise interventions significantly improved LMS (SMD = 1.07; 95% CI 0.73 to 1.41, p < 0.001), OCS (SMD = 0.79; 95% CI 0.32 to 1.26, p = 0.001), and SS (SMD = 0.72; 95% CI 0.45 to 0.98, p < 0.0001).

Conclusion

exercise interventions can effectively improve the FMS of children with ASD. The effects on LMS are considered as large effect sizes, while the effects on OCS and SS are considered as moderate effect sizes. These findings can inform clinical practice.

Systematic review registration

https://inplasy.com/inplasy-2022-12-0013/.
Mobile health in the specific management of first-episode psychosis: a systematic literature review
Authors: Maechling Claire , Yrondi Antoine , Cambon Amandine
Published: June 2023

Purpose

The purpose of this systematic literature review is to assess the therapeutic efficacy of mobile health methods in the management of patients with first-episode psychosis (FEP).

Method

The participants are patients with FEP. The interventions are smartphone applications. The studies assess the preliminary efficacy of various types of application.

Results

One study found that monitoring symptoms minimized relapses, visits to A&E and hospital admissions, while one study showed a decrease in positive psychotic symptoms. One study found an improvement in anxiety symptoms and two studies noted an improvement in psychotic symptoms. One study demonstrated its efficacy in helping participants return to studying and employment and one study reported improved motivation.

Conclusion

The studies suggest that mobile applications have potential value in the management of young patients with FEP through the use of various assessment and intervention tools. This systematic review has several limitations due to the lack of randomized controlled studies available in the literature.
Psychedelic therapy in the treatment of addiction: the past, present and future
Authors: Zafar Rayyan , Siegel Maxim , Harding Rebecca , Barba Tommaso , Agnorelli Claudio , Suseelan Shayam , Roseman Leor , Wall Matthew , Nutt David John , Erritzoe David
Published: June 2023

Psychedelic therapy has witnessed a resurgence in interest in the last decade from the scientific and medical communities with evidence now building for its safety and efficacy in treating a range of psychiatric disorders including addiction. In this review we will chart the research investigating the role of these interventions in individuals with addiction beginning with an overview of the current socioeconomic impact of addiction, treatment options, and outcomes. We will start by examining historical studies from the first psychedelic research era of the mid-late 1900s, followed by an overview of the available real-world evidence gathered from naturalistic, observational, and survey-based studies. We will then cover modern-day clinical trials of psychedelic therapies in addiction from first-in-human to phase II clinical trials. Finally, we will provide an overview of the different translational human neuropsychopharmacology techniques, including functional magnetic resonance imaging (fMRI) and positron emission tomography (PET), that can be applied to foster a mechanistic understanding of therapeutic mechanisms. A more granular understanding of the treatment effects of psychedelics will facilitate the optimisation of the psychedelic therapy drug development landscape, and ultimately improve patient outcomes.
Breath-centered virtual mind-body medicine reduces COVID-related stress in women healthcare workers of the Regional Integrated Support for Education in Northern Ireland: a single group study
Authors: Gerbarg Patricia L. , Dickson Felicity , Conte Vincent A. , Brown Richard P.
Published: June 2023

Background

During the COVID-19 pandemic, healthcare workers endured prolonged stress affecting their psychological well-being. Objectives: (1) Evaluate the effects of the Breath-Body-Mind Introductory Course (BBMIC) on COVID-related stress among employees of the Regional Integrated Support for Education, Northern Ireland, (2) Reduce the risk of adverse effects from COVID-related stress, and (3) Evaluate the effects of BBMIC on indicators of psychophysiological states and the consistency with hypothesized mechanisms of action.

Methods

In this single group study, a convenience sample of 39 female healthcare workers completed informed consent and baseline measures: Perceived Stress Scale (PSS), Stress Overload Scale-Short (SOS-S), and Exercise-Induced Feelings Inventory (EFI). Following the online BBMIC 4 h/day for 3 days and the 6 week solo (20 min/day) and group practice (45 min weekly), repeat testing plus the Indicators of Psychophysiological State (IPSS) and Program Evaluation were obtained.

Results

Baseline (T1) mean PSS score was significantly elevated compared to a normative sample: PSS = 18.2 vs. 13.7 (p < 0.001) and improved significantly 11 weeks post-BBMIC (T4). SOS-S mean score declined from 10.7(T1) to 9.7 at 6 week post-test (T3). The SOS-S proportion of High Risk scores found in 22/29 participants (T1), dropped to 7/29 (T3). EFI mean subscale scores improved significantly from T1 to T2 and T3 for Revitalization (p < 0.001); Exhaustion (p < 0.002); and Tranquility (p < 0.001); but not Engagement (p < 0.289).

Conclusion

Among RISE NI healthcare workers affected by COVID-related stress, participation in the BBMIC significantly reduced scores for Perceived Stress, Stress Overload, and Exhaustion. EFI Revitalization and Tranquility scores significantly improved. More than 60% of participants reported moderate to very strong improvements in 22 indicators of psychophysiological state, e.g., tension, mood, sleep, mental focus, anger, connectedness, awareness, hopefulness, and empathy. These results are consistent with the hypothesized mechanisms of action whereby voluntarily regulated breathing exercises change interoceptive messaging to brain regulatory networks that shift psychophysiological states of distress and defense to states of calmness and connection. These positive findings warrant validation in larger, controlled studies to extend the understanding of how breath-centered Mind-body Medicine practices could mitigate adverse effects of stress.
Preventive health examinations: protocol for a prospective cross-sectional study of German employees aged 45 to 59 years (Ü45-check)
Authors: Kalski Linda , Greiß Franziska , Hartung Johannes J. , Hafermann Lorena , Hofmann Maja A. , Wolfarth Bernd
Published: June 2023

Objective

Early identification of health-related risk factors is of great importance for maintaining workability. Screening examinations can help to detect diseases at an early stage and provide more needs-based recommendations. This study aims (1) to assess the individual need for prevention or rehabilitation based on preventive health examinations compared to a questionnaire survey, (2) to assess the results of the preventive health examinations compared to the Risk Index – Disability Pension (RI-DP), (3) to assess the results of the questionnaire survey compared to the RI-DP, (4) to assess the general health status of the sample (target population > 1,000) in German employees aged 45–59, (5) to identify the most common medical conditions. A further study question aims, and (6) to investigate the general health status of the specific occupational groups.

Methods

Comprehensive diagnostics including medical examination, anamnesis, anthropometric measurements, bioelectrical impedance analysis (BIA), handgrip strength, resting electrocardiogram (ECG), resting blood pressure, pulse wave velocity (PWV), and laboratory blood analyses added by a questionnaire are conducted. The research questions are analyzed in an exploratory manner.

Results and conclusion

We expect that the results will allow us to formulate recommendations regarding screening for prevention and rehabilitation needs on a more evidence-based level.

Clinical Trial Registration: DRKS ID: DRKS00030982.
IRIDA-ARIES Genomics, a key player in the One Health surveillance of diseases caused by infectious agents in Italy
Authors: Knijn Arnold , Michelacci Valeria , Gigliucci Federica , Tozzoli Rosangela , Chiani Paola , Minelli Fabio , Scavia Gaia , Ventola Eleonora , Morabito Stefano
Published: June 2023

Pathogen genomics is transforming surveillance of infectious diseases, deepening our understanding of evolution and diffusion of etiological agents, host-pathogen interactions and antimicrobial resistance. This discipline is playing an important role in the development of One Health Surveillance with public health experts of various disciplines integrating methods applied to pathogen research, monitoring, management and prevention of outbreaks. Especially with the notion that foodborne diseases may not be transmitted by food only, the ARIES Genomics project aimed to deliver an Information System for the collection of genomic and epidemiological data to enable genomics-based surveillance of infectious epidemics, foodborne outbreaks and diseases at the animal-human interface. Keeping in mind that the users of the system comprised persons with expertise in a wide variety of domains, the system was expected to be used with a low learning curve directly by the persons target of the analyses' results, keeping the information exchange chains as short as possible. As a result, the IRIDA-ARIES platform (https://irida.iss.it/) provides an intuitive web-based interface for multisectoral data collection and bioinformatic analyses. In practice, the user creates a sample and uploads the Next-generation sequencing reads, then an analysis pipeline is launched automatically performing a series of typing and clustering operations fueling the information flow. Instances of IRIDA-ARIES host the Italian national surveillance system for infections by Listeria monocytogenes (Lm) and the surveillance system for infections by Shigatoxin-producing Escherichia coli (STEC). As of today, the platform does not provide tools to manage epidemiological investigations but serves as an instrument of aggregation for risk monitoring, capable of triggering alarms on possible critical situations that might go unnoticed otherwise.
Repurposing agricultural waste as low-cost cultured meat scaffolds
Authors: Perreault Luke R. , Thyden Richard , Kloster Jack , Jones Jordan D. , Nunes Jordan , Patmanidis Andriana A. , Reddig David , Dominko Tanja , Gaudette Glenn R.
Published: June 2023

Growing meat in vitro using tissue engineering and bioproduction techniques (cellular agriculture) has become an increasingly promising solution to the global food security challenge. Our lab has established methods to cultivate bovine muscle tissue on decellularized plants, representing a viable low-cost, sustainable method to grow meat on edible scaffolds. Most work in this area has focused on the use of edible plant materials (i.e., spinach leaves, apple, broccoli) with inherent economic value. Harvest waste such as corn husk or jackfruit represent abundant sources of cellulose for scaffold production and may be a viable alternative. The present study aims to investigate production of cultured meat through tissue engineering and bioproduction on decellularized, edible samples of corn husk and jackfruit rind. Corn husks and jackfruit rinds were exposed to immersion decellularization. DNA quantification and histological analysis demonstrated sufficient decellularization (0.17 ± 0.06 and 0.07 ± 0.00 ug DNA/g tissue for corn husk and jackfruit rinds, respectively). Following decellularization, corn husk scaffold stiffnesses decreased from 56.67±16.71 MPa to 12.95±2.43 MPa in fiber-aligned direction, while jackfruit decreased from 7.54 ±2.42 MPa to 2.47±1.47 MPa. Seeded scaffolds with bovine satellite cells (BSCs) (11.45±2.24 ug/ul lysate/Gram) and avian (QM7s) (12.90±1.99 ug/ul lysate/Gram) demonstrated increased protein yields on jackfruit scaffolds. QM7 cultured on corn husk scaffolds yielded increased protein but PBSCs seeded on corn husks did not yield protein content higher than controls (QM7 on corn husk: 16.28±3.55, PBSCs on corn husks: 9.57±1.56 ug/ul lysate/Gram, control: 6.35±1.43 ug/ul lysate/Gram). Additionally, cell transfer from scaffold to scaffold (bead-to-bead transfer) was observed on corn husk scaffolds in a dynamic environment. These results suggest that decellularized harvest waste scaffolds may aid in realization of cultured meat products that will contribute to a more robust and environmentally sustainable food supply.
How green is an urban tree? The impact of species selection in reducing the carbon footprint of park trees in Swedish cities
Authors: Lind Erik , Prade Thomas , Sjöman Deak Johanna , Levinsson Anna , Sjöman Henrik
Published: June 2023

Introduction

Planting trees in urban areas can mitigate some of the emissions generated in cities by carbon sequestration (annual uptake of CO2 through the process of photosynthesis) and carbon storage (amount of carbon stored in the tree's biomass throughout its lifespan). The aim of this study was to calculate the carbon footprint from nursery production to final establishment of different tree species grown for planting in urban parks in a northern European context.

Material and methods

The analysis included a cradle-to-gate approach and investigated the amount of carbon the adult trees needed to sequester in order to compensate for initial carbon emissions and which temporal perspectives are of concern. Greenhouse gas emissions were estimated based on an inventory of consumption of fuels, energy, materials and other production inputs during cultivation, delivery, planting and establishment of three different tree species in three different locations in Sweden. The tree species considered in the analysis (Salix alba, Quercus rubra, Pinus sylvestris) were selected due to significant differences in their growth rates. Salix alba is a competitive strategist in resource-rich habitats, and is proficient at converting these resources into vigorous growth. Pinus sylvestris is a pronounced stress strategist with good ability to handle resource-limited habitats, and invests in traits accordingly, resulting in significantly slower development. Quercus rubra has its main distribution in cool and moderately resource-rich habitats, but has relatively high stress tolerance and can be considered intermediate between the other two species in terms of growth rate.

Results and discussion

The results showed that within 16 years of planting, all species in all three cities, except Pinus sylvestris planted in Umeå, compensated for initial carbon emissions, i.e. showed net absorption of CO2 after emissions from cultivation, delivery, planting and establishment of the trees had been deducted. There was a clear link between the time by which compensation of initial carbon emissions was achieved and growth rate of the different species, with the fast-growing Salix alba showing the best results. The single largest source of emissions among all activities carried out during cultivation, delivery and planting of all species, regardless of the city in which they were planted, was fuel consumption during tree planting.
Haploidentical stem cell transplantation with post-transplant cyclophosphamide challenges and outcome from a tertiary care center in Lebanon
Authors: El Cheikh Jean , Bidaoui Ghassan , Sharrouf Layal , Zahreddine Ammar , Massoud Radwan , Nehme Rita , Kreidieh Nabila , Moukalled Nour , Abou Dalle Iman , Mahfouz Rami , Bazarbachi Ali
Published: June 2023

This letter describes the experience of the American University of Beirut Medical Center in Lebanon with haploidentical stem cell transplant (haplo-SCT) for hematological malignancies in adult patients. Haplo-SCT made it possible through universal and rapid donor availability for most of the adult patients with leukemia or lymphoma not only in the Middle East but also globally. Moreover, the use of post-transplant cyclophosphamide (PTCy) and reduced intensity conditioning (RIC) regimens when indicated improved the outcome and decreased the toxicity of haploidentical stem cell transplant.RIC regimens also allowed its use in the elderly population. Patients from throughout the Middle East come to our center, the American university of Beirut Medical Center, to receive this transformative type of stem cell transplant. In this paper, we discuss the results of haplo-SCT with PTCy done on adult patients with hematological malignancies in our center from 2015 to 2021. The results are encouraging and show that haplo-SCT should be considered more often in the Middle Eastern countries. The subgroup analysis showed the importance of achieving complete remission of the disease prior to transplant to improve outcomes in our center. There is a paucity of literature on the outcomes of haplo-SCT in the Middle East which may contribute to the limited number of centers that offer this type of SCT. Herein, we aim to fill this gap in the hopes of encouraging the implementation of this potentially curative modality of treatment to a larger extent in the Middle East.
Arterial oxygenation and acid–base status before and during oxygen supplementation in captive European bison (Bison bonasus) immobilized with etorphine-acepromazine-xylazine
Authors: Gardoni Nino , Björck Sven , Morelli Jacopo , Evans Alina L. , Barros Daniela S. B. , Wiklund Rikard , Græsli Anne Randi , Thiel Alexandra , Arnemo Jon M. , Lian Marianne
Published: June 2023

Chemical immobilization of captive European bison (Bison bonasus) is often required for veterinary care, transportation, or husbandry practices playing an important role in conservation breeding and reintroduction of the species. We evaluated the efficiency and physiological effects of an etorphine-acepromazine-xylazine combination with supplemental oxygen in 39 captive European bison. Animals were darted with a combination of 1.4 mg of etorphine, 4.5 mg of acepromazine, and 20 mg of xylazine per 100 kg based on estimated body mass. Arterial blood was sampled on average 20 min after recumbency and again 19 min later and analyzed immediately with a portable i-STAT analyzer. Simultaneously, heart rate, respiratory rate, and rectal temperature were recorded. Intranasal oxygen was started after the first sampling at a flow rate of 10 mL.kg−1.min−1of estimated body mass until the end of the procedure. The initial mean partial pressure of oxygen (PaO2) was 49.7 mmHg with 32 out of 35 sampled bison presenting with hypoxemia. We observed decreased respiratory rates and pH and mild hypercapnia consistent with a mild respiratory acidosis. After oxygen supplementation hypoxemia was resolved in 21 out of 32 bison, but respiratory acidosis was accentuated. Bison immobilized with a lower initial drug dose required supplementary injections during the procedure. We observed that lower mean rectal temperatures during the immobilization event were significantly associated with longer recovery times. For three bison, minor regurgitation was documented. No mortality or morbidity related to the immobilizations were reported for at least 2 months following the procedure. Based on our findings, we recommend a dose of 0.015 mg.kg−1etorphine, 0.049 mg.kg−1acepromazine, and 0.22 mg.kg−1xylazine. This dose reduced the need for supplemental injections to obtain a sufficient level of immobilization for routine management and husbandry procedures in captive European bison. Nevertheless, this drug combination is associated with development of marked hypoxemia, mild respiratory acidosis, and a small risk of regurgitation. Oxygen supplementation is strongly recommended when using this protocol.
Appropriate particle size of rice straw promoted rumen fermentation and regulated bacterial microbiota in a rumen simulation technique system
Authors: Li Zhiqing , Qiu Huijing , Lan Xinyi , Wang Zuo , Shen Weijun , Wan Fachun , Xiao Dingfu , He Jianhua
Published: June 2023

The purpose of this study is to reveal the effects of different particle sizes of rice straw on the rumen protozoa count, nutrient disappearance rate, rumen fermentation, and microbial community in a rumen simulation technique (RUSITEC) system. In this experiment, a single-factor random trial design was adopted. According to the different particle sizes of rice straw, there were three treatments with three replies in each treatment. Three kinds of goat total mixed ration (TMR), with the same nutrients were used to carry out a 10 days in vitro fermentation experiment using the rumen simulation system developed by Hunan Agricultural University, including 6 days the pretrial period and 4 days formal period. This study found that the organic matter disappearance rate, concentrations of total volatile fatty acids (VFAs), acetate, propionate, and iso-butyrate were greatest in the 4 mm group (p < 0.05). There were no significant differences in the alpha diversity, among the three groups (p > 0.05). The relative abundance of Treponema and Ruminococcus of the 2 mm group increased; the relative abundance of Butyrivibrio and Prevotella in samples increased in the 4 mm group. In addition, the results of correlation analysis showed that Prevotella and Ruminococcus was positively correlated with butyrate, ammonia-N, dOM and d ADF (p < 0.05) and negatively correlated with valerate (p < 0.05); Oscillospira was positively correlated with valerate (p < 0.01) and negatively correlated with propionate, butyrate, ammonia-N, dOM and dADF (p < 0.05). The present results imply that compared to the other groups, rice straw particle size of 4 mm may improve the disappearance rate of nutrients and promote the production of volatile fatty acids by regulating ruminal microorganisms.
Development and evaluation of a fish feed mixture containing the probiotic Lactiplantibacillus plantarum prepared using an innovative pellet coating method
Authors: Chomová Natália , Pavloková Sylvie , Sondorová Miriam , Mudroňová Dagmar , Fečkaninová Adriána , Popelka Peter , Koščová Jana , Žitňan Rudolf , Franc Aleš
Published: June 2023

Introduction

Due to the intensification of fish farming and the associated spread of antimicrobial resistance among animals and humans, it is necessary to discover new alternatives in the therapy and prophylaxis of diseases. Probiotics appear to be promising candidates because of their ability to stimulate immune responses and suppress the growth of pathogens.

Methods

The aim of this study was to prepare fish feed mixtures with various compositions and, based on their physical characteristics (sphericity, flow rate, density, hardness, friability, and loss on drying), choose the most suitable one for coating with the selected probiotic strain Lactobacillus plantarum R2 Biocenol™ CCM 8674 (new nom. Lactiplantibacillus plantarum). The probiotic strain was examined through sequence analysis for the presence of plantaricin- related genes. An invented coating technology based on a dry coating with colloidal silica followed by starch hydrogel containing L. plantarum was applied to pellets and tested for the viability of probiotics during an 11-month period at different temperatures (4°C and 22°C). The release kinetics of probiotics in artificial gastric juice and in water (pH = 2 and pH = 7) were also determined. Chemical and nutritional analyses were conducted for comparison of the quality of the control and coated pellets.

Results and discussion

The results showed a gradual and sufficient release of probiotics for a 24-hour period, from 104 CFU at 10 mi up to 106 at the end of measurement in both environments. The number of living probiotic bacteria was stable during the whole storage period at 4°C (108), and no significant decrease in living probiotic bacteria was observed. Sanger sequencing revealed the presence of plantaricin A and plantaricin EF. Chemical analysis revealed an increase in multiple nutrients compared to the uncoated cores. These findings disclose that the invented coating method with a selected probiotic strain improved nutrient composition and did not worsen any of the physical characteristics of pellets. Applied probiotics are also gradually released into the environment and have a high survival rate when stored at 4°C for a long period of time. The outputs of this study confirm the potential of prepared and tested probiotic fish mixtures for future use in in vivo experiments and in fish farms for the prevention of infectious diseases.
Do Conservative Agricultural Practices Improve the Functional Biological State of Legume-Based Cropping Systems?
Authors: Aschi Amira , Riah-Anglet Wassila , Recous Sylvie , Bailleul Caroline , Aubert Michaël , Trinsoutrot-Gattin Isabelle
Published: June 2023

This study evaluated the response of soil microbial functions to the presence and placement of faba beans in crop rotations (rotation without legumes versus rotations with faba beans as the crop previous to wheat, with the faba beans sown three years before the wheat crops) combined with two tillage systems (conventional and reduced tillage). The study included 36 wheat-producing plots, and two agricultural practice types were defined: high-frequency–low-intensity (HF–LI) and low-frequency–high-intensity (LF–HI). The results demonstrated a significant increase in the total carbon and nitrogen (N) content under reduced tillage. Furthermore, the general path analysis suggested that arylamidase and β-glucosidase activities significantly affect N fluxes. The enzyme activities were modified by changing the soil’s physicochemical properties. These findings highlighted the significance of introducing legumes as the crop preceding wheat, especially when applying conventional tillage. Moreover, it was revealed that farmers’ management of these conservative practices is a leading factor in regulating soil functions. Pesticides and inorganic fertilization inputs were classified as HF–LI practices, while organic matter (OM) inputs and liming treatments were qualified as LF–HI practices. For instance, LF–HI practices (OM inputs and liming) directly and indirectly influenced the soil functions related to the N cycle, while HF–LI practices (pesticide, inorganic N fertilization, and previous crops) resulted in fewer soil function changes.
Could Fibrinogen Concentration Be a Useful Indicator of Cattle Herd Health Status? Approaches to Setting Reference Values
Authors: Milczak Andrzej , Abramowicz Beata , Szczepanik Marcin , Madany Jacek , Wrześniewska Karolina , Buczek Krzysztof , Staniec Marta , Żółkiewski Paweł , Kurek Łukasz
Published: June 2023

Fibrinogen is used in the diagnosis of inflammation as an acute phase protein. The research objective set by the authors of this study was to assess the applicability of fibrinogen concentration measurement in the blood plasma of the peak of lactating dairy cows through the evaluation of the reference values by using the functional PT-derived (Prothrombin Time-derived) method. Materials and methods: The study was carried out on 259 HF (Holstein-Friesian) and white-backed cows. The animals were clinically healthy. Fibrinogen concentration was determined by automated PT-derived method. Fibrinogen concentrations were calculated as the mean of duplicate samples. Samples with differences between duplicate results greater than 5% were rejected. Results: In the group of HF cows, the average fibrinogen concentration was 11.75 ± 4.80 g/L. In white-backed cows, it was 9.53 ± 4.79 g/L. At total of 76.01% of the results of the fibrinogen concentration in HF cows and 82.05% of the results obtained in the group of white-backed cows were within the ±1 SD (Standard Deviation) range. Conclusions: Based on our own research, the PT-derived method may be applied in order to determine the concentration of fibrinogen in cattle herds in animal health monitoring studies. An individual laboratory should focus more on verifying reference intervals established elsewhere.
Theoretical Calculations and Experimental Studies of Power Loss in Dual-Clutch Transmission of Agricultural Tractors
Authors: Ahn Hyoung-Jong , Park Young-Jun , Kim Su-Chul , Choi Chanho
Published: June 2023

Recent carbon neutrality policies have led to active research in the agricultural tractor sector to replace internal combustion engines, making it imperative to minimize power losses to improve efficiency. Dual-clutch transmissions (DCTs) have been employed in agricultural tractors primarily due to their short shift time and smooth shift feel. However, DCTs have a relatively large number of components and complex structures owing to spatial constraints, making it challenging to predict power losses. Therefore, to predict DCT power losses, this study defined oil churning by considering the structural characteristics and oil circulation and comparing and analyzing the theoretical calculation and test results of power losses at different oil levels. Power loss was calculated based on ISO standards and fluid viscosity theory, and tests were performed to verify. We calculated power losses based on the defined oil churning of a DCT in agricultural tractors and confirmed that their consistency in test results improved when reflecting the lubrication state, considering the structural features and oil circulation. In addition, the factors contributing to power loss under low- and high-speed conditions were analyzed by calculating the power loss for each component.
Bnt05G007257, a Novel NAC Transcription Factor, Predicts Developmental and Synthesis Capabilities of Fiber Cells in Ramie (Boehmeria nivea L.)
Authors: Bai Xuehua , Fu Yafen , Wang Xin , Chen Guangyao , Wang Yanzhou , Liu Tongying , Li Guang , Zhu Siyuan
Published: June 2023

NAC transcription factors are one of the largest transcription factor families in plants, and they play a key role in the growth and development of a secondary cell wall. Despite the fact that ramie is well-known for its high fiber yield, the role of NAC transcription factors in ramie secondary cell wall synthesis and fiber development remains unknown. In this study, based on our previous study, we describe the characterization, physicochemical property analysis, protein structure and function prediction, subcellular localization, and functional validation of Bnt05G007257, which encodes an NAC transcription factor from ramie, in transgenic A. thaliana. Our findings show that the open reading frame of Bnt05G007257 was 1035 bp long and encodes for a protein comprising 344 amino acids, having a relative molecular mass of 39.0945 kDa and a theoretical isoelectric point of 6.55. The secondary structure of the encoded protein mainly consisted of random coiling, with a typical conserved structural domain of NAC. The phylogenetic tree revealed that Bnt05G007257 is a homolog of the NAC transcription factor SND2, which regulates secondary wall biosynthesis in A. thaliana. Subcellular localization showed that Bnt05G007257 was tentatively predicted to be localized in the cytoplasm. Furthermore, in stem sections, the secondary wall fiber cells’ thickness in Bnt05G007257 transgenic plants was 31.50% thicker than that in wild-type plants, and the radial width was significantly increased by approximately 21.75%. This indicates that the NAC family homolog Bnt05G007257 may have the potential function of promoting fiber cell development and secondary cell wall synthesis, providing a theoretical basis for the selection of high-fiber-yielding ramie varieties in the future.
Effects of Incorporating Different Proportions of Humic Acid into Phosphate Fertilizers on Phosphorus Migration and Transformation in Soil
Authors: Jing Jianyuan , Zhang Shuiqin , Yuan Liang , Li Yanting , Zhang Yingqiang , Ye Xinxin , Zhang Ligan , Xiong Qizhong , Wang Yingying , Zhao Bingqiang
Published: June 2023

Incorporating humic acid (HA) into phosphate fertilizers to produce HA-enhanced phosphate fertilizers (HAPs) can improve the migration and availability of fertilizer-derived phosphorus (P) in soil. However, the optimal proportion of HA remains inconsistent. Here, we investigated the effects of HAPs with different HA proportions (0.1–10% w/w) on water-soluble P fixation rate, P migration, P transformation, and soil microorganisms, and analyzed the main P forms in HAP using Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. The results showed that incorporating 0.1% HA had no impact on P migration and transformation, whereas incorporating 0.5–10% HA increased the migration distance and cumulative migration of fertilizer-derived P by 0–5 mm and 17.1–30.3%, respectively, compared with conventional phosphate fertilizer (CP). Meanwhile, HAPs with 0.5–10% HA significantly reduced the water-soluble P fixation rate by 18.3–25.6%, and significantly increased the soil average available P (AP) content in 0–40 mm soil layer around the P application site by 6.2–12.9% relative to CP, partly due to the phosphate monoesters in HAPs. Clustering analysis revealed that 0.5% HA had similar effects relative to higher HA proportions (1% and 5%), and the inhibition of HAP with 0.5% HA on bacteria and fungi was also greater than that of CP due to the high concentration of soil P. Overall, 0.5% was determined to be the optimal amount of HA for HAP production, which provided a theoretical basis for the development of high-efficiency phosphate fertilizer.
Monitoring of Cropland Abandonment Based on Long Time Series Remote Sensing Data: A Case Study of Fujian Province, China
Authors: Wu Jiayu , Jin Shaofei , Zhu Gaolong , Guo Jia
Published: June 2023

Farmland is the basis for human survival and development. The phenomenon of cropland abandonment has seriously affected national agricultural production and food security. In this study, remote sensing monitoring of abandoned cropland is carried out based on multisource time series remote sensing data using the Google Earth Engine (GEE) cloud platform. Landsat and Sentinel-2 time series data from 2010–2021 were used to obtain monthly synthetic cloud-free image sets in combination with cropland plot data. The time series farmland probability dataset was generated using the random forest classification method. The LandTrendr algorithm was used to extract and analyse the time series cropland probability dataset. Finally, this study also explored the drivers of change in abandoned cropland in Fujian Province. The results show that (1) the LandTrendr algorithm can effectively extract abandoned farmland and avoid the impact of pseudovariation resulting from non-farmland categories. A total of 87.02% of the abandoned farmland was extracted in 2018; 87.50% of the abandoned farmland was extracted in 2020. (2) The abandoned area in Fujian Province fluctuated after a significant increase in 2012, with the abandoned area exceeding 30 thousand hectares. Since 2017, the abandoned area has decreased to slightly below 30 thousand hectares. (3) The regression results of the factors affecting abandoned cropland in Fujian Province show that the increase in the number of agricultural workers and the improvement in soil organic matter content will significantly reduce the area of abandoned cropland in Fujian Province, while the increase in the rate of urbanization, poor road accessibility, and insufficient irrigation conditions will increase the area of abandoned cropland. The results of this study are useful for conducting surveys of cropland abandonment and obtaining timely and accurate data on cropland abandonment. The results of this study are of great significance for the development of effective measures to stop the abandonment of cropland, and ensure the implementation of food security strategies.
PRRS Monitoring by Processing Fluids on Italian Swine Breeding Farms
Authors: Tonni Matteo , Romeo Claudia , Formenti Nicoletta , Boniotti Maria Beatrice , Guarneri Flavia , Colosio Livio , Andreoni Simone , Scali Federico , Alborali Giovanni Loris
Published: June 2023

The porcine reproductive and respiratory syndrome (PRRS) control strategy within swine breeding farms is based on herd classification relative to PRRSV infection status. This study aims to assess the efficacy of a monitoring plan based on processing fluids (PFs) by comparing it with the classification of herds based on the analysis of blood serum. Twenty-five breeding herds were enrolled in the study, with at least five consecutive batches sampled from each herd. Each batch was tested for PRRSV by RT-PCR performed on (i) pre-weaning blood serum from 30 piglets and (ii) PFs from all the male piglets in the batch. PRRS categories following the Holtkamp classification were assigned based on the results of each testing protocol. The two protocols assigned the same category to 18 out of 25 herds: while they showed perfect agreement in identifying positive unstable and stable herds, we observed some discrepancy in discriminating between low- and high-prevalence classes within unstable herds. PFs are thus a reliable sample to assign PRRS categories in Italian breeding herds characterized by widespread PRRSV circulation. However, in case of an unstable epidemiological scenario, we recommend the adoption of an integrated monitoring strategy that combines blood sampling with PFs.