bluuebunny/crossref_metadata_2025 huggingface dataset:
Dex modulates the balance of water-electrolyte metabolism by depressing the expression of AVP in PVN
Authors: Yang Wenzhi , Li Hao , Cheng Zhongle , Lu You , Li Wuli , Feng Jun , Wang Liecheng , Cheng Juan
Published: August 2022

Dexmedetomidine (Dex) is a highly selective α2 adrenergic agonist used in clinical anesthesia. Studies have shown that Dex can act on the collecting duct and reduce the body’s water reabsorption, thereby increasing water discharge. However, the specific mechanism of Dex on water homeostasis remains unclear. The hypothalamus is the regulatory center of water and salt balance and secretes related neurochemical hormones, such as arginine vasopressin (AVP), to regulate the discharge of water and salt. The paraventricular nucleus (PVN) and supraoptic nucleus (SON) in the hypothalamus are also considered to be the key targets of the thirst loop. They are responsible for the secretion of AVP. The suprachiasmatic nucleus (SCN) is also one of the brain regions where AVP neurons are densely distributed in the hypothalamus. This study used C57BL/6J mice for behavior, immunofluorescence, and blood analysis experiments. Our results showed that Dex could not only depress the expression of AVP in the PVN but also reduce serum AVP concentration. The animal water intake was decreased without impairing the difference in food consumption and the urine excretion was enhanced after the intraperitoneal injection of Dex, while AVP supplementation restored the water intake and inhibited the urine excretion of mice in the Dex group. In addition, the renin-angiotensin-aldosterone system is vital to maintaining serum sodium concentration and extracellular volume. We found that serum sodium, serum chloride, serum aldosterone (ALD) concentration, and plasma osmolality were decreased in the Dex group, which inhibited water reabsorption, and the plasma osmolarity of mice in the Dex group supplemented with AVP was significantly higher than that in Dex group. We also found that Dex significantly increased the concentration of blood urea nitrogen and decreased the concentration of creatinine within the normal range of clinical indicators, indicating that there was no substantive lesion in the renal parenchyma. These results showed that Dex could modulate the balance of water-electrolyte metabolism by depressing the expression of AVP in PVN without impairing renal function.
Renovation as innovation: Repurposing human antibacterial peptide LL-37 for cancer therapy
Authors: Lu Fatai , Zhu Yingkang , Zhang Guodong , Liu Zunpeng
Published: August 2022

In many organisms, antimicrobial peptides (AMPs) display wide activities in innate host defense against microbial pathogens. Mammalian AMPs include the cathelicidin and defensin families. LL37 is the only one member of the cathelicidin family of host defense peptides expressed in humans. Since its discovery, it has become clear that they have pleiotropic effects. In addition to its antibacterial properties, many studies have shown that LL37 is also involved in a wide variety of biological activities, including tissue repair, inflammatory responses, hemotaxis, and chemokine induction. Moreover, recent studies suggest that LL37 exhibits the intricate and contradictory effects in promoting or inhibiting tumor growth. Indeed, an increasing amount of evidence suggests that human LL37 including its fragments and analogs shows anticancer effects on many kinds of cancer cell lines, although LL37 is also involved in cancer progression. Focusing on recent information, in this review, we explore and summarize how LL37 contributes to anticancer effect as well as discuss the strategies to enhance delivery of this peptide and selectivity for cancer cells.
Characterization of mesenchymal stromal cells physical properties using acoustic radiation force
Authors: Bellebon Ludovic , Sugier Hugo R. , Larghero Jérôme , Peltzer Juliette , Martinaud Christophe , Hoyos Mauricio , Aider Jean-Luc
Published: August 2022

Microfluidics-based technologies are emerging solutions towards cell separation process. They rely on various physical principles, such as dielectrophoretic force, hydrodynamic force, and acoustic force. In order to take advantage of these approaches for cell sorting, it is crucial to characterize cell biophysical properties, i.e. size, density, compressibility or acoustic contrast factor in the case of acoustophoresis. Various techniques exist to measure those features, including acoustofluidics methods. However, previously described approaches do not allow the determination of all the physical parameters of a given cell. For this study, a 330 μm deep acoustic cavity has been designed, together with an optical technique, to measure the size, the acoustic contrast factor and the density of cells to finally determine their compressibility. A defocusing technique is used to assess the velocity of sedimentation and acoustic focusing of individual cells which can lead to their physical properties using the analytic expression of the Acoustic Radiation Force. This method is used to investigate the evolution of the biophysical properties of Mesenchymal Stromal Cells (MSCs), isolated from two different tissues from different donors, from one passage to the other. Our results highlighted an important heterogeneity of acoustic contrast factor and compressibility among cells from a same donor but also inter-donor. Nevertheless, from passage to passage, the acoustic contrast factor and compressibility of MSCs converge and homogenize at the fourth passage. On the other hand, the density of MSCs remains homogenous from passage to passage and between different donors. Using this technique, it was possible to successfully assess the different biophysical properties of mesenchymal stromal cells using a single setup based on acoustic levitation. The results confirm the necessity to use such a technique to measure the cells properties. It also demonstrates the large heterogeneity of donor/patient-derived cells, in contrast with cultured cells whose properties homogenize during the successive cultures. As a consequence, cell separation processes will be more complex for patient/donor derived cells than for cultured cells.
Effects of omecamtiv mecarbil on the contractile properties of skinned porcine left atrial and ventricular muscles
Authors: Nakanishi Tomohiro , Oyama Kotaro , Tanaka Hiroyuki , Kobirumaki-Shimozawa Fuyu , Ishii Shuya , Terui Takako , Ishiwata Shin’ichi , Fukuda Norio
Published: August 2022

Omecamtiv mecarbil (OM) is a novel inotropic agent for heart failure with systolic dysfunction. OM prolongs the actomyosin attachment duration, which enhances thin filament cooperative activation and accordingly promotes the binding of neighboring myosin to actin. In the present study, we investigated the effects of OM on the steady-state contractile properties in skinned porcine left ventricular (PLV) and atrial (PLA) muscles. OM increased Ca2+ sensitivity in a concentration-dependent manner in PLV, by left shifting the mid-point (pCa50) of the force-pCa curve (ΔpCa50) by ∼0.16 and ∼0.33 pCa units at 0.5 and 1.0 μM, respectively. The Ca2+-sensitizing effect was likewise observed in PLA, but less pronounced with ΔpCa50 values of ∼0.08 and ∼0.22 pCa units at 0.5 and 1.0 μM, respectively. The Ca2+-sensitizing effect of OM (1.0 μM) was attenuated under enhanced thin filament cooperative activation in both PLV and PLA; this attenuation occurred directly via treatment with fast skeletal troponin (ΔpCa50: ∼0.16 and ∼0.10 pCa units in PLV and PLA, respectively) and indirectly by increasing the number of strongly bound cross-bridges in the presence of 3 mM MgADP (ΔpCa50: ∼0.21 and ∼0.08 pCa units in PLV and PLA, respectively). It is likely that this attenuation of the Ca2+-sensitizing effect of OM is due to a decrease in the number of “recruitable” cross-bridges that can potentially produce active force. When cross-bridge detachment was accelerated in the presence of 20 mM inorganic phosphate, the Ca2+-sensitizing effect of OM (1.0 μM) was markedly decreased in both types of preparations (ΔpCa50: ∼0.09 and ∼0.03 pCa units in PLV and PLA, respectively). The present findings suggest that the positive inotropy of OM is more markedly exerted in the ventricle than in the atrium, which results from the strongly bound cross-bridge-dependent allosteric activation of thin filaments.
Overview of biofertilizers in crop production and stress management for sustainable agriculture
Authors: Chaudhary Parul , Singh Shivani , Chaudhary Anuj , Sharma Anita , Kumar Govind
Published: August 2022

With the increase in world population, the demography of humans is estimated to be exceeded and it has become a major challenge to provide an adequate amount of food, feed, and agricultural products majorly in developing countries. The use of chemical fertilizers causes the plant to grow efficiently and rapidly to meet the food demand. The drawbacks of using a higher quantity of chemical or synthetic fertilizers are environmental pollution, persistent changes in the soil ecology, physiochemical composition, decreasing agricultural productivity and cause several health hazards. Climatic factors are responsible for enhancing abiotic stress on crops, resulting in reduced agricultural productivity. There are various types of abiotic and biotic stress factors like soil salinity, drought, wind, improper temperature, heavy metals, waterlogging, and different weeds and phytopathogens like bacteria, viruses, fungi, and nematodes which attack plants, reducing crop productivity and quality. There is a shift toward the use of biofertilizers due to all these facts, which provide nutrition through natural processes like zinc, potassium and phosphorus solubilization, nitrogen fixation, production of hormones, siderophore, various hydrolytic enzymes and protect the plant from different plant pathogens and stress conditions. They provide the nutrition in adequate amount that is sufficient for healthy crop development to fulfill the demand of the increasing population worldwide, eco-friendly and economically convenient. This review will focus on biofertilizers and their mechanisms of action, role in crop productivity and in biotic/abiotic stress tolerance.
Investigation of the impact of supplemental reflective films to improve micro-light climate within tomato plant canopy in solar greenhouses
Authors: Liu Anhua , Henke Michael , Li Yiming , Zhang Yue , Xu Demin , Liu Xingan , Li Tianlai
Published: August 2022

The non-uniform growth and development of crops within Chinese Solar Greenhouses (CSG) is directly related to the micro-light climate within canopy. In practice, reflective films are used to improve micro-light climate within plant canopy by homogenizing light distribution and so increasing total plant light interception. However, as to our knowledge, the contributions to light distribution within canopy have not been investigated for passive reflector like reflective films. Field experiments dealing with light conditions and growth behavior over time, are complicated to carry out, time-consuming and hard to control, while however, accurate measurements of how reflective films influence the micro-light climate of canopy are an essential step to improve the growth conditions for any crop. Here, we propose a supplementary light strategy using reflective films to improve light distribution within plant canopy. Based on the example of CSG, a 3D greenhouse model including a detailed 3D tomato canopy structure was constructed to simulate the influence of supplementary reflective films to improve micro-light climate. Comparison of measured solar radiation intensity with predicted model data demonstrated that the model could precisely predict light radiation intensity over time with different time points and positions in the greenhouse. A series of reflective film configurations were investigated based on features analysis of light distribution in the tomato canopy on sunny days using the proposed model. The reflective film configuration scheme with the highest impact significantly improved the evenness of horizontal and vertical light distribution in tomato canopy. The strategy provided here can be used to configure reflective films that will enhance light conditions in CSG, which can be applied and extended in different scenarios.
Mechanisms of plant cell wall surveillance in response to pathogens, cell wall-derived ligands and the effect of expansins to infection resistance or susceptibility
Authors: Narváez-Barragán Delia A. , Tovar-Herrera Omar E. , Guevara-García Arturo , Serrano Mario , Martinez-Anaya Claudia
Published: August 2022

Cell wall integrity is tightly regulated and maintained given that non-physiological modification of cell walls could render plants vulnerable to biotic and/or abiotic stresses. Expansins are plant cell wall-modifying proteins active during many developmental and physiological processes, but they can also be produced by bacteria and fungi during interaction with plant hosts. Cell wall alteration brought about by ectopic expression, overexpression, or exogenous addition of expansins from either eukaryote or prokaryote origin can in some instances provide resistance to pathogens, while in other cases plants become more susceptible to infection. In these circumstances altered cell wall mechanical properties might be directly responsible for pathogen resistance or susceptibility outcomes. Simultaneously, through membrane receptors for enzymatically released cell wall fragments or by sensing modified cell wall barrier properties, plants trigger intracellular signaling cascades inducing defense responses and reinforcement of the cell wall, contributing to various infection phenotypes, in which expansins might also be involved. Here, we review the plant immune response activated by cell wall surveillance mechanisms, cell wall fragments identified as responsible for immune responses, and expansin’s roles in resistance and susceptibility of plants to pathogen attack.
Plant-DTI: Extending the landscape of TF protein and DNA interaction in plants by a machine learning-based approach
Authors: Ruengsrichaiya Bhukrit , Nukoolkit Chakarida , Kalapanulak Saowalak , Saithong Treenut
Published: August 2022

As a sessile organism, plants hold elaborate transcriptional regulatory systems that allow them to adapt to variable surrounding environments. Current understanding of plant regulatory mechanisms is greatly constrained by limited knowledge of transcription factor (TF)–DNA interactions. To mitigate this problem, a Plant-DTI predictor (Plant DBD-TFBS Interaction) was developed here as the first machine-learning model that covered the largest experimental datasets of 30 plant TF families, including 7 plant-specific DNA binding domain (DBD) types, and their transcription factor binding sites (TFBSs). Plant-DTI introduced a novel TFBS feature construction, called TFBS base-preference, which enhanced the specificity of TFBS to DBD types. The proposed model showed better predictive performance with the TFBS base-preference than the simple binary representation. Plant-DTI was validated with 22 independent ChIP-seq datasets. It accurately predicted the measured DBD-TFBS pairs along with their TFBS motifs, and effectively predicted interactions of other TFs containing similar DBD types. Comparing to the existing state-of-art methods, Plant-DTI prediction showed a figure of merit in sensitivity and specificity with respect to the position weight matrix (PWM) and TSPTFBS methods. Finally, the proposed Plant-DTI model helped to fill the knowledge gap in the regulatory mechanisms of the cassava sucrose synthase 1 gene (MeSUS1). Plant-DTI predicted MeERF72 as a regulator of MeSUS1 in consistence with the yeast one-hybrid (Y1H) experiment. Taken together, Plant-DTI would help facilitate the prediction of TF-TFBS and TF-target gene (TG) interactions, thereby accelerating the study of transcriptional regulatory systems in plant species.
Biofortification of oil quality, yield, and nutrient uptake in Indian mustard (Brassica juncea L.) by foliar application of boron and nitrogen
Authors: Dhaliwal Salwinder Singh , Sharma Vivek , Shukla Arvind Kumar , Kaur Manmeet , Verma Vibha , Sandhu Prabhjodh Singh , Alsuhaibani Amnah Mohammed , Gaber Ahmed , Hossain Akbar
Published: August 2022

Indian mustard (Brassica juncea L.) is an essential oilseed crop that offers important nutrients to human beings. However, the concurrent micronutrient deficiencies including boron (B), sulfur (S), and nitrogen (N) could pose a significant threat to public health. Therefore, this study was conducted at the Punjab Agricultural University, Ludhiana, with nine treatments, i.e., T1-Control (recommended NPK only), T2- borax (0.5%) at flowering, T3-borax (1.0%) at flowering,T4- borax (0.5%) + urea (1.0%) at flowering,T5-borax (1.0%) + urea (1.0%) at flowering, T6-borax (0.5%) at flowering + capsule formation, T7-borax (1.0%) at flowering + capsule formation, T8-borax (0.5%) + urea (1.0%) at flowering + capsule formation, T9-borax (1.0%) + urea (1.0%) at flowering + Capsule formation, replicated three times in a randomized block design for 2 years (2020–2021 and 2021–2022). The foliar application of borax (1.0%) + urea (1.0%) at the flowering and capsule formation stage (treatment T9) was highly efficient in increasing food quality parameters such as crude fiber, total soluble solids (TSS), and protein content with maximum values of 3.77, 24.9, and 27.53%, respectively. Also, maximum yields of seed as well as stover for treatment T9 were 1.376 and 6.625 kg ha−1, respectively. Similarly, the results for B, S, and N concentrations in seed (27.71 mg kg−1, 17.69 mg kg−1, and 2.35%), as well as stover (25.92 mg kg−1, 17.31 mg kg−1, and 0.33%), were maximum in treatment T9. Also, B, S, and N uptake by seed (38.18 g ha−1, 24.40 g ha−1, and 32.05 Kg ha−1) and stover (172.55 g ha−1, 115.44 g ha−1, and 21.99 Kg ha−1) were maximum for the treatment T9 involving borax (1.0%) + urea (1.0%) at the flowering and capsule formation stage. Whereas, the concentration and uptake decreased in the treatments involving the sole application of borax and urea. Therefore, the application of borax (1.0%) and urea (1.0%) at the flowering and capsule formation stage significantly improved the quality parameters, seed and stover yield, nutrient concentration, and uptake over control and could be used to alleviate the B, S, and N deficiency in Indian mustard.
Validation of reliability and validity of the Chinese version of the active-empathic listening scale
Authors: Guo Hang , Lin Lemin , Jia Ziming , Sun Jiaying , Zhuang Zisen , Duan Lisa , Sun Jiangnan
Published: August 2022

Background

Active-empathic listening (AEL) is the active and emotional involvement of a listener that can take place in at least three key stages of the listening process. Bodie has developed and validated a self-reported, 11-item, three-factor active-empathic listening scale (AELS) in English with good reliability (Cronbach’s alpha = 0.86) to assess AEL abilities. Nevertheless, a Chinese version of the AELS had not been established and validated yet.

Objective

The objective of the present study was to examine the reliability and validity of the Chinese version of the AELS.

Methods

After translating the scale into the Chinese version, 834 college students completed the test. After 4 weeks, 206 participants were tested again on the Chinese AELS to examine retest reliability. The critical ratio method and the item-total correlations were used for the item analysis. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were performed to examine the construct validity. The internal consistency of the scale was analyzed with Cronbach’s alpha and McDonald’s Omega. Interclass correlation coefficient (ICC) was used to examine the scale’s retest reliability. The Interpersonal Reactivity Index (IRI) was used to examine the convergent validity. Pearson correlation analysis was conducted.

Results

Each item of the Chinese AELS had a good discrimination, and the item-total correlation of each item ranged from 0.51 to 0.73. EFA extracted three factors with characteristic root values greater than 1, which could explain 70.72% of the total variance. CFA indicated an adequate fit of the three-factor model (χ2/df = 2.250, root mean square error of approximation [RMSEA] = 0.055, the comparative fit index [CFI] = 0.971, the Tucker-Lewis index [TLI] = 0.959, and the goodness of fit index [GFI] = 0.959). The internal consistency reliability was acceptable (sensing: α = 0.79/ω = 0.78, processing: α = 0.83/ω = 0.83, responding: α = 0.79/ω = 0.79, and AELS: α = 0.87/ω = 0.87). Retest reliability of the scale at 4-week intervals by an ICC was 0.563. The Chinese AELS was significantly correlated with each dimension of IRI.

Conclusion

The reliability and validity of the Chinese AELS met the basic psychometrics requirements. Therefore, the scale can be potentially used to assess the active empathic listening abilities of people in China.
Anxiety and depression among adult tobacco users during the COVID-19 restrictions in India
Authors: Bassi Shalini , Nazar Gaurang P. , Joshi Nishigandha , Sharma Nitika , Pandian Aishwarya , Deepa Mohan , Mohan Sailesh , Patel Shivani A. , Ali Mohammed K. , McNeill Ann , Tandon Nikhil , Mohan Viswanathan , Prabhakaran Dorairaj , Arora Monika
Published: August 2022

Background

The world witnessed a highly contagious and deadly disease, COVID-19, toward the end of 2019. India is one of the worst affected countries. We aimed to assess anxiety and depression levels among adult tobacco users and people who recently quit tobacco during COVID-19 lockdown in India.

Materials and methods

The study was conducted across two Indian cities, Delhi and Chennai (July-August, 2020) among adult tobacco users (n = 801). Telephonic interviews were conducted using validated mental health tools (Patient Health Questionnaire-PHQ-9 and Generalized Anxiety Disorder-GAD-7) to assess the anxiety and depression levels of the participants. Descriptive analysis and multiple logistic regression were used to study the prevalence and correlates of depression and anxiety.

Results

We found that 20.6% of tobacco users had depression symptoms (3.9% moderate to severe); 20.7% had anxiety symptoms (3.8% moderate to severe). Risk factors associated with depression and anxiety included food, housing, and financial insecurity.

Conclusion

During COVID-19 lockdown, mental health of tobacco users (primarily women) was associated with food, housing and financial insecurity. The Indian Government rightly initiated several health, social and economic measures to shield the most vulnerable from COVID-19, including a ban on the sale of tobacco products. It is also necessary to prioritize universal health coverage, expanded social security net, tobacco cessation and mental health services to such vulnerable populations during pandemic situations.
Residents' perception and worldview about radon control policy in Canada: A pro-equity social justice lens
Authors: Khan Selim M. , Gomes James , Nicol Anne-Marie
Published: August 2022

Radon is a potent indoor air pollutant, especially in radon prone areas and in countries with long winters. As the second top lung carcinogen, radon is disproportionately affecting certain population subgroups. While many provinces have taken sporadic actions, the equity issue has remained unaddressed across all policy measures. Attempts to enforce radon guidelines and enact building regulations without considering residents' views have proved ineffective. Research linking residents' radon risk perception and worldviews regarding radon control policy is lacking in Canada. We applied mixed (quantitative and qualitative) methods in a pro-equity social justice lens to examine the variations in residents' risk perception, access to risk communication messages, and worldviews about risk management across the sociodemographic strata. Triangulation of the quantitative and qualitative findings strengthened the evidence base to identify challenges and potential solutions in addressing the health risk through upstream policy actions. Enacting radon control policy requires actions from all levels of governments and relevant stakeholders to ensure equal opportunities for all residents to take the preventive and adaptive measures. Small sample size limited the scope of findings for generalization. Future studies can examine the differential impacts of radon health risk as are determined by various sociodemographic variables in a representative national cohort.
Hepatitis C antibody prevalence and behavioral correlates in people who inject drugs attending harm reduction services in Lisbon, Portugal
Authors: Curado Adriana , Nogueira Paulo Jorge , Virgolino Ana , Santa Maria João , Mendão Luís , Furtado Cristina , Antunes Francisco
Published: August 2022

The hepatitis C virus (HCV) infection is an important public health problem, affecting millions of people worldwide. People who inject drugs (PWID) are at increased risk of HCV infection due to, among other factors, widespread unsafe injecting practices, such as sharing of infected equipment or unprotected sexual practices. In Portugal, there is a lack of data regarding the proportion of infected persons through injecting drug use. This study aimed to evaluate the anti-HCV prevalence and behavioral correlates of infection in PWID attending harm reduction services in the Metropolitan Area of Lisbon, Portugal. A cross-sectional study with a purposive sample of PWID was undertaken between March 2018 and March 2020. Participants were recruited through the harm-reduction services of a nongovernmental organization. A rapid diagnostic test for anti-HCV screening was performed. Data on drug consumption history and current practices, past HCV testing, care and treatment history, and knowledge regarding hepatitis C were also collected through a questionnaire applied by trained inquirers. A total of 176 PWID participated in this study. An overall prevalence of 70.5% of anti-HCV positive in this population was found. Those with an anti-HCV positive testing result tended to start consuming at a younger age and have a higher consumption of benzodiazepines in the last 30 days. Sharing needles and other injecting material is a frequent risk behavior among this group. Also, they are more likely to have attended an opioid agonist treatment and to have undertaken previous hepatitis C and HIV tests in the past. This study represents an important effort to better understand the HCV prevalence and behavioral correlates of infection among PWID in Portugal, as well as to better estimate those in need of HCV treatment.
Validation of the fear of COVID-19 scale in a central Balkan country - Serbia
Authors: Zivkovic Zaric Radica , Zaric Milan , Canovic Petar , Jankovic Slobodan , Stojadinovic Milorad , Zornic Nenad , Nesic Jelena , Spasic Marko , Jovanovic Dalibor , Jug Martina , Jakovljevic Stefan , Pejcic Ana
Published: August 2022

Validation of the fear of introduction

High levels of fear of COVID-19 may be associated with increased levels of stress, anxiety, and depression, as well as decreased resilience and life expectancy.

Objective

This study aimed to translate and confirm the Serbian version of the Fear of COVID-19 scale as well as to investigate its psychometric properties.

Methods

The translation and intercultural adaptation of the Fear of COVID-19 scale was performed by the leading standard of the International Society for Pharmacoeconomics and Outcome Research. When the distribution was normal, the Kolmogorov-Smirnov test was used. The reliability of the Serbian version of FCV-19S was tested by measuring the internal consistency through the value of Cronbach's alpha.

Results

The original version of the Fear of COVID-19 scale was tested on a sample of 256 subjects with a mean age of 25.38 ± 12.47. The Cronbach's alpha value was 0.864. We divided the scale by the split-half method (Spearman-Brown), and the value of the coefficient for the questionnaire as a whole was 0.882. Divergent criterion validity was tested through the non-parametric correlation between the scores of the Fear of COVID-19 scale and the Fear of Hospitalization scale. A score of the Fear of COVID-19 scale was calculated as the sum of each question for each of the respondents. Convergent criterion validity was tested through the non-parametric correlation between the scores of the Fear of COVID-19 scale and the Emotional Regulation Questionnaire.

Conclusion

The validated version of the scale in Serbia complements versions available in other cultures and other languages and facilitates global studies related to mental health during the COVID-19 pandemic.
The positive association between internal migration and hospitalization among the older adults in China: Regional heterogeneity and chronic disease management
Authors: Zhong Huixiang , Yang Jin , Zhao Na , Li Xu , Zhang Yanli
Published: August 2022

Background

Post-retirement migrants are rapidly increasing in China, but the impact of internal migration on hospitalization among older adults remains under-researched. Understanding this impact is essential for health policies development and improvement. This study aims to identify the most vulnerable population, evaluate the association between migration and hospitalization, and discuss potential causes of the association.

Methods

14,478 older adults were extracted from the 2018 to 2019 Chinese Longitudinal Healthy Longevity Survey (CLHLS) database and divided into four groups according to migration experience and age at migration: non-migrants, pre-adulthood migrants, pre-retirement migrants, and post-retirement migrants. Post-retirement migrants were key research subjects. We employed Pearson's chi-square test to compare group differences in outcome and covariates, and multivariate logistic regression analysis to examine the association between migration and hospitalization by regions and chronic conditions.

Results

Significant intergroup differences were observed in demographic characteristics, socioeconomic factors, health habits, and health-related factors. Post-retirement migrants displayed following characteristics: female predominance (61.6%; 1,472/2,391), tending toward urban areas (80.9%; 1,935/2,391), and the highest prevalence rate of chronic disease (46.7%; 1,116/2,391). Urban migrants in eastern China were more likely to be hospitalized (OR = 1.65; 95% CI: 1.27–2.15), especially those who were diagnosed with chronic disease (OR = 1.51; 95% CI: 1.04–2.19) or with unconfirmed chronic conditions (OR = 1.98; 95% CI: 1.36–2.89).

Conclusions

Internal migration is associated with the hospitalization of post-retirement migrants moving to eastern China. Improved chronic disease management and early interventions might lower the hospitalization. Effective policies should be formulated to reduce the disparity in primary care services across China, thereby facilitating the access of migrants to these services.
A university-clustered tuberculosis outbreak during the COVID-19 pandemic in eastern China
Authors: Wu Jizhou , Zhu Limei , Yu Jiaxi , Liu Qiao , Ding Xiaoyan , Lu Peng , Wu Yunliang , Sun Jiansheng , Martinez Leonardo , Lu Wei , Wang Jianming
Published: August 2022

During the COVID-19 pandemic in 2020, a tuberculosis outbreak occurred in a university in eastern China, with 4,488 students and 421 staff on the campus. A 19-year-old student was diagnosed in August 2019. Later, the first round of screening was initiated among close contacts, but no active cases were found. Till September 2020, four rounds of screening were performed. Four rounds of screening were conducted on September 9, November 8, November 22-25 in 2019 and September 2020, with 0, 5, 0 and 43 cases identified, respectively. A total of 66 active tuberculosis were found in the same university, including 4 sputum culture-positive and 7 sputum smear-positive. The total attack rate of active tuberculosis was 1.34% (66/4909). The whole-genome sequencing showed that the isolates belonged to the same L2 sub-specie and were sensitive to all tested antituberculosis drugs. Delay detection, diagnosis and report of cases were the major cause of this university tuberculosis epidemic. More attention should be paid to the asymptomatic students in the index class. After the occurrence of tuberculosis cases in schools, multiple rounds of screening should be carried out, and preventive therapy should be applied in a timely manner.
Scalable and heterogenous mobile robot fleet-based task automation in crowded hospital environments—a field test
Authors: Valner Robert , Masnavi Houman , Rybalskii Igor , Põlluäär Rauno , Kõiv Erik , Aabloo Alvo , Kruusamäe Karl , Singh Arun Kumar
Published: August 2022

In hospitals, trained medical staff are often, in addition to performing complex procedures, spending valuable time on secondary tasks such as transporting samples and medical equipment; or even guiding patients and visitors around the premises. If these non-medical tasks were automated by deploying mobile service robots, more time can be focused on treating patients or allowing well-deserved rest for the potentially overworked healthcare professionals. Automating such tasks requires a human-aware robotic mobility system that can among other things navigate the hallways of the hospital; predictively avoid collisions with humans and other dynamic obstacles; coordinate task distribution and area coverage within a fleet of robots and other IoT devices; and interact with the staff, patients and visitors in an intuitive way. This work presents the results, lessons-learned and the source code of deploying a heterogeneous mobile robot fleet at the Tartu University Hospital, performing object transportation tasks in areas of intense crowd movement and narrow hallways. The primary use-case is defined as transporting time-critical samples from an intensive care unit to the hospital lab. Our work builds upon Robotics Middleware Framework (RMF), an open source, actively growing and highly capable fleet management platform which is yet to reach full maturity. Thus this paper demonstrates and validates the real-world deployment of RMF in an hospital setting and describes the integration efforts.
Risk factors and prognosis of liver metastasis in gallbladder cancer patients: A SEER-based study
Authors: Fang Cheng , Li Wenhui , Wang Qingqiang , Wang Ruoran , Dong Hui , Chen Junjie , Chen Yong
Published: August 2022

Background

Liver metastasis is a common complication in gallbladder cancer (GBC). We design this study to develop models for predicting the development of liver metastasis in GBC patients and evaluate the risk of mortality in these patients with liver metastasis.

Methods

GBC patients from Surveillance Epidemiology and End Results (SEER) between 2010 and 2016 were included in this study. Logistic regression was performed to discover risk factors and construct predictive models for liver metastasis in GBC patients. Cox regression was utilized to find risk factors of mortality in GBC patients with liver metastasis. The area under the receiver operating characteristic curve (AUC) was calculated to evaluate the performance of the constructed predictive models.

Results

Multivariate logistic regression confirmed that T stage, N stage, and tumor grade were risk factors for liver metastasis in GBC patients. Composed of these factors, the model for predicting the development of liver metastasis had AUCs of 0.707 and 0.657 in the training cohort and testing cohort, respectively. Multivariate Cox regression showed that surgery of the primary site and chemotherapy were independently associated with the mortality of GBC patients with liver metastasis. Composed of these two factors, the predictive model for 1-year mortality of GBC patients with liver metastasis had AUCs of 0.734 and 0.776 in the training cohort and testing cohort, respectively.

Conclusion

The predictive models that we constructed are helpful for surgeons to evaluate the risk of liver metastasis in GBC patients and the survival condition of those with liver metastasis. Surgery of the primary site and chemotherapy should be provided for GBC with liver metastasis.
Treatment outcomes of penetrating abdominal injury requiring laparotomy at Hiwot Fana Specialized University Hospital, Harar, Ethiopia
Authors: Abdulkadir Adnan , Mohammed Burka , Sertse Elias , Mengesha Melkamu Merid , Gebremichael Mathewos Alemu
Published: August 2022

Background

Penetrating abdominal injury (PAI) is a public health problem and accounts for significant mortality and disability in both developing and developed countries. It often causes damage to internal organs, resulting in shock and infection. In this study, we assessed the outcomes of PAI and factors associated with post-surgical outcomes including surgical site infection (SSI) and in-hospital death.

Methods

An institution-based cross-sectional study was conducted from 15 January to January 30, 2020, using a standard checklist to review the clinical charts of patients who presented to Hiwot Fana Specialized University Hospital (HFSUH) with PAI and underwent laparotomy between January 2015 and September 2019. Descriptive statistics were used to describe the characteristics of patients, and odds ratios (ORs) with a 95% confidence interval (CI) were reported for factors included in binary logistic regression. The statistical significance was declared at a P-value <0.05.

Results

A total of 352 charts of patients with PAI were reviewed. A majority of them (84.9%) were males and the mean age was 26.5 years. The anterior abdomen was the most common site of injury, accounting for 285 patients (81%), 329 patients (93.5%) suffered organ injury, 204 (62%) had a single organ injury, and 125 (38%) had more than one organ injury. The leading injured organs were small intestines 194 (55.1%), followed by the colon 88 (25%) and liver 40 (11.4%). The magnitude of SSI and hospital death was 84 (23.9%) and 12 (3.4%), respectively. Patients above 45 years of age (AOR = 2.9, 95% CI: 1.2, 9.2), with fluid collection (AOR = 2.7, 95% CI: 1.2, 5.9), colostomy (AOR = 3.9, 95% CI: 1.9, 7.8), body temperature >37.5 °C (AOR = 3.8,95% CI:1.9,7.6), and Hgb < 10 mg/dl (AOR = 7.4, 95% CI: 3.4,16.1) had a higher likelihood of SSI. Those patients admitted to the intensive care unit (AOR = 21.3, 95% CI: 1.1, 412.3) and who underwent damage control surgery (AOR = 9.6, 95% CI: 1.3, 73.3) had a higher likelihood of mortality.

Conclusions

SSI and death among patients with PAI were high. Age, fluid collection, colostomy, body temperature, and hemoglobin level were statistically associated with SSI, and intensive care unit and damage control surgery were statistically associated with death. Therefore, health professionals working in surgical wards should consider these factors to alleviate SSI and prevent death. Broadly speaking, the guidelines of the World Society of Emergency Surgery (WSES) should consider these factors in their recommendations.
A universal incision for robot-assisted thoracic surgery
Authors: Jiao Jia , Guo Jinbao , Zhao Jia , Li Xiangnan , Du Ming
Published: August 2022

Objective

This paper aimed to design and explore the versatility of the incision for the robot-assisted thoracic surgery.

Methods

The concept of universal incision was designed and put forward. The clinical data of 342 cases of robot-assisted thoracic surgery were summarized, including sex, age, clinical diagnosis, operative method, operative time, conversion to thoracotomy, intraoperative blood loss, number of lymph node dissections, postoperative hospital stays, postoperative pathology, and postoperative complications of the patients.

Results

The 342 cases of robot-assisted surgery included 178 pulmonary surgery cases (94 lobectomy cases, 75 segmentectomy cases, 6 wedge resection cases, and 3 sleeve lobectomy cases), 112 esophageal surgery cases (107 McKeown approach cases and 5 esophageal leiomyoma resection cases), and 52 mediastinal tumor cases (42 anterior mediastinum cases and 10 posterior mediastinum cases). Among these, two cases were converted to thoracotomy (both esophageal cases), and the rest were successful with no massive intraoperative bleeding and no perioperative death.

Conclusion

The universal incision of robot-assisted thoracic surgery is safe and feasible and is suitable for most cases of thoracic surgery.
Case report: Large follicular thyroid carcinoma with multiple cervical lymph node metastases
Authors: Ye Fei , Liao Liyan , Tan Wanlin , Gong Yi , Li Xiaodu , Niu Chengcheng
Published: August 2022

Introduction

Follicular thyroid carcinoma (FTC) rarely metastasizes to regional lymph nodes, as they mainly metastasize through hematogenous route; in particular, a large FTC with only lateral lymph node metastasis and without distant metastasis has rarely been reported.

Case report

We present a 66-year-old male patient with a progressively growing thyroid for more than 20 years, causing tracheal compression and narrowing. Neck ultrasonography, computed tomography (CT), magnetic resonance (MR) imaging and positron emission tomography–computed tomography (PET/CT) were carried out to obtain images of the thyroid and surrounding tissues. Total thyroidectomy and cervical lateral and central lymph node dissection were undertaken, and histopathological, and immunohistochemical evaluations and molecular pathology confirmed the diagnosis of FTC with multiple cervical lymph node metastases.

Conclusion

We have reported a rare case of large FTC with diffuse nodal involvement but no distant metastases. We present the thyroid ultrasound, neck CT, MR and whole body PET/CT.
New Approach to the Public Authorities’ Activities Development in the Crop Insurance System: Lithuanian Case
Authors: Drejeris Rolandas , Rusteika Martynas
Published: August 2022

This article substantiates the structure of the crop insurance system and describes the participants of the insurance system and their activities. The positive impact of crop insurance development on all participants of the system has also been clarified. The aim of the article is to present a methodology for assessing substantiated directs of activity for public authorities in order to make more active crop insurance system performance. The application of the proposed methodology can help to activate crop insurance processes and to expand farmers’ activities and achieve better commercial results of insurance companies. It has been proven that it is beneficial for the public authorities to reinsure farmers’ crops and to refuse to pay direct payments to farmers for the losses incurred. The criteria selected for the assessment of the development directions are relevant to all participants of the insurance system. The research was carried out in a region of Lithuania in which the composition of agricultural business entities corresponds to the situation in the whole agricultural sector of the country. The identification of the insurance system participants and their functions, as well as the use of an expert assessment method with the application of quantitative data processing, showed directions for the activation of crop insurance activities.
DepthFormer: A High-Resolution Depth-Wise Transformer for Animal Pose Estimation
Authors: Liu Sicong , Fan Qingcheng , Liu Shanghao , Zhao Chunjiang
Published: August 2022

Animal pose estimation has important value in both theoretical research and practical applications, such as zoology and wildlife conservation. A simple but effective high-resolution Transformer model for animal pose estimation called DepthFormer is provided in this study to address the issue of large-scale models for multi-animal pose estimation being problematic with limited computing resources. We make good use of a multi-branch parallel design that can maintain high-resolution representations throughout the process. Along with two similarities, i.e., sparse connectivity and weight sharing between self-attention and depthwise convolution, we utilize the delicate structure of the Transformer and representative batch normalization to design a new basic block for reducing the number of parameters and the amount of computation required. In addition, four PoolFormer blocks are introduced after the parallel network to maintain good performance. Benchmark evaluation is performed on a public database named AP-10K, which contains 23 animal families and 54 species, and the results are compared with the other six state-of-the-art pose estimation networks. The results demonstrate that the performance of DepthFormer surpasses that of other popular lightweight networks (e.g., Lite-HRNet and HRFormer-Tiny) when performing this task. This work can provide effective technical support to accurately estimate animal poses with limited computing resources.
Effect of Rabbit Urine on the Larval Behavior, Larval Mortality, Egg Hatchability, Adult Emergence and Oviposition Preference of the Fall Armyworm (Spodoptera frugiperda J.E. Smith)
Authors: Kemunto Diana , Omuse Evanson R. , Mfuti David K. , Tamiru Amanuel , Hailu Girma , Rwiza Ignath , Belayneh Yeneneh T. , Subramanian Sevgan , Niassy Saliou
Published: August 2022

The fall armyworm (FAW) (Spodoptera frugiperda J.E. Smith) is a major cereal pest threatening food security in Africa. African smallholder farmers apply various indigenous pest management practices, including rabbit urine; however, there is no scientific evidence for its efficacy. The FAW eggs, first, second and third instar larvae and moths were exposed to rabbit urine-treated maize leaves alongside untreated maize leaves (control). More FAW larvae (46.0–70.0%) remained on the untreated leaves than those (27.0–43.0%) on the rabbit urine-treated leaves. Rabbit urine caused 6.4 and 12.8% damage reduction of the second and third instars, respectively, 24 h post-exposure. Rabbit urine significantly reduced the survival of FAW, had a lethal time (LT50) of 5.0, 7.3 and 8.7 days and a lethal dose (LD50) of 48, 94, and 55% for the first, second and third instars, respectively. Egg hatchability and adult emergence were reduced by 55.0 and 13.3%, respectively. The FAW female moths laid more eggs on the rabbit urine-treated plants (647 ± 153 eggs) than they did on the untreated plants (72 ± 64 eggs). This study confirms farmers’ assertions about using rabbit urine to manage FAW. For successful integration into the FAW IPM package, additional studies on the chemistry of rabbit urine, the behavioral response and the field might be required.
HBRNet: Boundary Enhancement Segmentation Network for Cropland Extraction in High-Resolution Remote Sensing Images
Authors: Sheng Jiajia , Sun Youqiang , Huang He , Xu Wenyu , Pei Haotian , Zhang Wei , Wu Xiaowei
Published: August 2022

Cropland extraction has great significance in crop area statistics, intelligent farm machinery operations, agricultural yield estimates, and so on. Semantic segmentation is widely applied to remote sensing image cropland extraction. Traditional semantic segmentation methods using convolutional networks result in a lack of contextual and boundary information when extracting large areas of cropland. In this paper, we propose a boundary enhancement segmentation network for cropland extraction in high-resolution remote sensing images (HBRNet). HBRNet uses Swin Transformer with the pyramidal hierarchy as the backbone to enhance the boundary details while obtaining context. We separate the boundary features and body features from the low-level features, and then perform a boundary detail enhancement module (BDE) on the high-level features. Endeavoring to fuse the boundary features and body features, the module for interaction between boundary information and body information (IBBM) is proposed. We select remote sensing images containing large-scale cropland in Yizheng City, Jiangsu Province as the Agricultural dataset for cropland extraction. Our algorithm is applied to the Agriculture dataset to extract cropland with mIoU of 79.61%, OA of 89.4%, and IoU of 84.59% for cropland. In addition, we conduct experiments on the DeepGlobe, which focuses on the rural areas and has a diversity of cropland cover types. The experimental results indicate that HBRNet improves the segmentation performance of the cropland.