bluuebunny/crossref_metadata_2025 huggingface dataset:
Shoot organogenesis and somatic embryogenesis from leaf and petiole explants of endangered Euryodendron excelsum
Authors: Xiong Yuping , Chen Shuangyan , Wu Teng , Wu Kunlin , Li Yuan , Zhang Xinhua , Silva Jaime A. Teixeira , Zeng Songjun , Ma Guohua
Published: June 2022

Euryodendron excelsum H.T. Chang is a first class of rare and endangered woody plant endemic to China. Leaves and petioles from the axillary shoots in vitro were used as explants to culture on the different PGR WPM medium and establish an efficient shoot proliferation and plant regeneration system. Callus and 3–6 adventitious shoots or somatic embryos were induced. WPM supplemented with 1.0 mg/L 2,4-D induced callus that then dedifferentiated into shoots and somatic embryos on different media, including PGR-free WPM. However, only adventitious shoots formed on WPM with 1.0 mg/L of cytokinins, 6-benzyladenine (BA), kinetin (KIN) or thidiazuron (TDZ). When another cytokinin, zeatin, was used, somatic embryos were induced directly from leaves and petioles. Adventitious roots could be induced from both explants on WPM with 1.0 mg/L α-naphthaleneacetic acid (NAA). Somatic embryos cultured in PGR-free WPM or WPM with 0.2 mg/L NAA developed roots. Plantlets derived from somatic embryos were transferred to a peat: sand (1:1, v/v) substrate, with 64.3% survival after 30 d, and 54.6% after 90 d. Callus clumps with adventitious shoot buds that were transferred to WPM with 1.0 mg/L BA and 0.2 mg/L NAA developed mean 3.3 multiple shoots. Callus-derived shoots regenerated and rooted successfully (100%) on agar-free vermiculite-based WPM with 0.5 µM NAA after 30 d. Plantlets transplanted to peat soil: vermiculite (1:1, v/v) displayed highest survival (96.7%) after three months. This protocol allows for the mass propagation of tissue-cultured plants and the conservation of this rare and endangered genetic resource.
Historical temperature variability in a representative high-latitude region in the monsoon-continental climate transition zone in China
Authors: Wei Yingnan , Ma Long , Sun Bolin , Zhang Jing , Jing Quanpu , Xing Jianjun
Published: June 2022

Global warming has exacerbated the instability of the climate and has led to the frequent occurrence of extreme cold and warm events. In this study, we constructed a standardized tree-ring width chronology of Korean spruce (Picea koraiensis Nakai) growing in a representative high-latitude region in the monsoon-continental climate transition zone in China from 1845 to 2016 and used it to analyse the response of the radial growth of Korean spruce to climatic factors such as temperature and precipitation. The results show that the annual mean temperature was the dominant climatic factor affecting the growth of Korean spruce. Hence, we reconstructed the annual mean temperature series of this region spanning the past 172 years. The analysis results show that the study area experienced five warm periods and five cold periods in the past 172 years. Cold years were dominant before 1960s, while temperature continuously rose and changed drastically in the early 21st century. The reconstructed annual mean temperature series has variability cycles of 3 a, 7 a, 10–12 a, 15–22 a, and 30–40 a. The results of this reconstruction enrich the tree-ring database in the representative regions of the monsoon-continental climate transition zone in China and provide a reference for systematically understanding the climate change patterns in the representative regions of the transition zone.
Immune cellular patterns of distribution affect outcomes of patients with non-small cell lung cancer
Authors: Parra Edwin Roger , Zhang Jiexin , Jiang Mei , Tamegnon Auriole , Pandurengan Renganayaki , Behrens Carmen , Heymach John , Moran Cesar , Lee J. Jack , Gibbons Don , Wistuba Ignacio
Published: June 2022

Background
Study of the geographic distribution of cellular populations and their interaction with malignant cells in non-small cell lung cancer (NSCLC) is essential to understand the roles of cellular populations and potentially design new therapeutic approaches.
Material and Methods
We studied 225 formalin-fixed, paraffin-embedded tumor tissue samples from patients with stage I-III NSCLC—142 adenocarcinomas and 83 squamous cell carcinomas—placed in tissue microarrays. Twenty-three markers were used, including T-cell, B-cell, immune checkpoint, and myeloid cell markers, placed in five multiplex immunofluorescence panels. We used dimensional data reduction and cellular spatial distribution to identify cell phenotypes and their cellular distribution across panels. In addition, we analyzed associations between cellular spatial distribution patterns and clinicopathologic variables, tumor mutational status, and outcomes.
Results
Overall, we observed two patterns of cellular distribution—unmixed and mixed—related to T-cells, B-cells, granulocytic cells, and macrophages, and we detected various cellular interactions between malignant and immunosuppressive cells and various associations between cell distribution and clinicopathologic characteristics. Cellular distribution patterns and the distance from various cell phenotypes to malignant cells were associated with recurrence-free survival and overall survival in univariate and multivariable analysis.
Conclusions
Diverse populations of tumor cells and tumor-associated immune cell phenotypes can be present in NSCLC samples. Spatial distribution is a new tool to better understand the tumor microenvironment and help select therapeutic interventions.
Preparation of angiotensin I- converting enzyme (ACE) inhibitory peptides from Tieguanyin tea residue protein using two-step enzymatic hydrolysis
Authors: Ye Shufang , Luo Jinyan , Lin Jiarong , Meng Chun , Hong Jing
Published: June 2022

Angiotensin I-converting enzyme inhibitory (ACEI) peptides, released from precursor proteins in vitro and in vivo, have been paid increased attention in the prevention of high blood pressure. In this study, we attempted to prepare novel ACEI peptides from Tieguanyin tea residue protein (TTRP). Different proteases were used to hydrolyze TTRP to prepare ACEI peptides, neutral protease and alkaline protease hydrolysates exhibited higher ACEI activity and was chosen in further hydrolysis treatment. Response surface methodology (RSM) was used to optimize the enzymatic condition, and the results indicated that the optimal conditions of enzymatic hydrolysis were: 4% (W/V) of TTRP in water was hydrolyzed with 3500 U/g neutral protease at 50 ℃ and pH 6.5 for 2.5 h in the first step and then with 5000 U/g alkaline protease at 50 ℃ and pH 10.0 for 3 h in the second step༎Under the optimum condition, the ACE inhibitory rate of the final products was 88.45%±0.45% at the concentration of 1.0 mg/mL. Moreover, ACEI peptides derived from TTRP showed good stability under different temperatures, pHs, metal ions and gastrointestinal digestion. These results indicated that the TTRP has potential to be used for preparing ACEI peptides, providing the basis for improving the utilization efficiency for blood pressure reduction and benefit in economic value.
Limited efficacy of tocilizumab in adult patients with secondary hemophagocytic lymphohistiocytosis: A retrospective cohort study
Authors: Kim Ju Yeon , Kim Miso , Park Jin Kyun , Lee Eun Bong , Park Jun Won , Hong Junshik
Published: June 2022

Background: Interleukin (IL)-6 is one of the key cytokines in the pathogenesis of secondary hemophagocytic lymphohistiocytosis (sHLH); however, the efficacy and safety of tocilizumab (TCZ), a monoclonal IL-6 receptor antibody, in patients with sHLH is uncertain. Methods/Results: This study included 64 adult patients who were diagnosed with sHLH based on the HLH-2004 criteria. Patients were classified into two groups based on treatment regimen at baseline: tocilizumab (TCZ group, n=8) versus other treatments (control group), including HLH-2004 protocol (n=35), chemotherapy (n=7), glucocorticoid alone (n=8), and with other immunosuppressants (n=6). Primary outcome was overall 8-week survival. Baseline characteristics between the two groups were comparable. At day 56, one patient (12.5%) in the TCZ group and twenty-eight patients (51.9%) in the control group survived. Univariable and multivariable Cox proportional hazard analysis showed that TCZ significantly increased the risk of death (adjusted hazard ratio=5.55; 95% CI=2.13–14.49). The complete or partial response rate at day 14 was 44.6% in the control group, and nil in the TCZ group. In contrast, infectious complications occurred more frequently in the TCZ group than in the control group (14.3% vs. 50.0%). Conclusions: Our results suggest that tocilizumab has limited efficacy in treating adult patients with sHLH and could increase the risk of infectious complications compared to the conventional treatments.
The Value of Nurse-led Anthropometric and Oropharyngeal Measurements Combined with STOP-Bang Questionnaire in Screening for Obstructive Sleep Apnea in Patients with Acute Coronary Syndrome: A Prospective Cohort Study
Authors: Li Zexuan , Miao Hua , Zhang Siyu , Fan Jingyao , Yan Yan , Gong Wei , Zheng Wen , Wang Xiao , Que Bin , Ai Hui , Zhang Lixin , Nie Shaoping
Published: June 2022

Background: Obstructive sleep apnea (OSA) is a modifiable risk factor for acute coronary syndrome (ACS), with high prevalence but low diagnostic rates. Therefore, it is particularly important to develop strategies for better screening for OSA in newly admitted ACS patients. Methods: From March 2017 to October 2019, consecutive eligible patients with ACS underwent cardiorespiratory polygraphy during hospitalization. OSA was defined as an apnea-hypopnea index (AHI) ≥ 15 events/h. All anthropometric and oropharyngeal parameters are measured by specialist nurses. Results: Finally, 761 ACS patients were recruited in the present study. Prevalence of moderate/severe OSA was 53.2% based on diagnostic criteria of AHI ≥ 15. Correlation analysis illustrated that AHI was positively correlated with anthropometric characteristics. In the multivariate model, only micrognathia (OR 2.02, 95% CI 1.02-4.00, P = 0.044), waist circumference (OR 1.08, 95% CI 1.04-1.11, P < 0.001), and STOP-BANG Questionnaire (SBQ) score (OR 1.45, 95% CI 1.27-1.66, P < 0.001) were independently associated with the prevalence of OSA. Receiver operating characteristic curve (ROC) analysis showed that the area under curve (AUC) of multivariable joint diagnosis (waist circumference, micrognathia combined with SBQ) was significantly better than the AUC of Epworth Sleepiness Scale (ESS) and SBQ (p < 0.0001 and p = 0.0002, respectively), and the results showed that AUC was 0.728. Under the optimal truncation value, the sensitivity was 73%, and the specificity was 61%, which was higher than the single index. Finally, we also constructed a nomogram model based on multiple logistic regression, to easily determine the probability of OSA in ACS patients.Conclusions: The new screening tool has greater power than single questionnaire or measurements in screening of OSA among ACS patients.Trial registration: Clinicaltrials.gov identifier NCT03362385, registered December 5, 2017.
Does subjecting plants to water stress enhance biological nitrification inhibition potential of rice?
Authors: Dandeniya Warshi Shamila , Thies Janice E. , DiTommaso Antonio
Published: June 2022

Background and aims:
Biological nitrification inhibition (BNI) is a trait that could improve nitrogen-use efficiency of a crop. We studied varietal differences in BNI potential of rice at the vegetative phase when plants were subjected to water stress.
Methods
We obtained water-soluble root exudates (RE) and water-extracts of crushed roots (RT) from two week-old seedlings of five rice varieties grown continuously under adequate water or under water stress and assessed their ability to suppress the activity of Nitrosomonas europaea and the growth of lettuce seedlings. We also investigated how growing rice under continuously saturated (TC) and unsaturated (TU) soil moisture conditions affected potential nitrification rate (PNR) and the community structure of ammonia-oxidizing archaea and bacteria (AOA and AOB, respectively) in the rhizosphere compared with the bulk soil.
Results
We observed that only RT at ≥ 0.50 mg ml− 1suppressed the activity of N. europaea with significant (p < 0.05) differences among rice varieties. Subjecting rice varieties PI312777 and PI338046 to water stress significantly (p < 0.05) increased BNI of RT. Inhibition of lettuce root growth by RT was strongly correlated with its BNI (r = 0.83, p < 0.05). As revealed in T-RFLP profiles, varietal effects on AOA and AOB varied with moisture management. AOB were more responsive to soil moisture conditions than AOA.
Conclusions
The rice varieties studied produced compounds that could inhibit ammonia oxidizers, but the degree of BNI differed based on moisture stress faced by the crop. Lineages of rice varieties with known allelopathic potential would be promising candidates in a search for BNI potential.
Standardization of in vitro multiplication technique for Areca concinna, an endangered palm species for its conservation and utilization
Authors: Veluru Aparna , M Neema , Shil Sandip , Prakash Krishna , K Kavya , Karun Anitha
Published: June 2022

Areca concinna (Arecaceae) is an endangered plant species endemic to south western Sri Lanka. It is an important palm distributed primarily in Sri Lanka, India, and Southeast Asia and was listed under endangered plants species by IUCN due to habitat loss. Here a protocol was developed for clonal multiplication of A. concinna. Clonal propogation is essential for conservation and maintenance of endangered species. Immature inflorescence, immature embryos and mature embryos were tested for callogenesis and subsequent somatic embryogenesis in M72 basal media supplemented with the auxins 2, 4-D and Picloram. Of the three explants, somatic embryos were obtained only from mature embryos. Callus multiplication and somatic embryo formation in M72 basal media supplemented with 2, 4-D were found to be better over Picloram media. Serial transfer of explants from higher to lower concentrations was essential for sustaining the multiplication of callus as well as for induction of somatic embryos. Somatic embryos grown inY3 or 1/2 Y3 basal medium supplemented with BAP (1 mg/L), NAA (0.5 mg/L), and IBA (0.25 mg/L) exhibited shoot initiation and plantlet development. This protocol has its application in rapid multiplication of A. concinna palms thereby enhancing the possibility of conservation of the endangered palm.
Antibacterial activity of super-paramagnetic iron oxide nanoparticles against Edwardsiella tarda from water
Authors: Kumar Munish , Gupta Gyandeep , Varghese Tincy , Srivast Prem Prakash , Gupta Subodh
Published: June 2022

The current study was conducted to evaluate the antibacterial activity of super-paramagnetic iron oxide nanoparticles (SPIONs) against Edwardsiella tarda from water. The antibacterial activity of different concentrations of SPIONs viz. 0, 6.25, 12.5, 25, 50, 100, 200, 500 and 1000 µM were assessed in sterilized distilled water with different initial bacterial loads of E. tarda viz. 1×103, 1×104, 1×105, 1×106 and 1×107 CFU ml− 1 at various exposure time (15, 30, 45 and 60 min). At lowest bacterial load 1×103 CFU ml− 1, > 98% bacteria was removed by 500 µM of SPIONs concentration group after 15 min exposure, which was increased up to 100% by increasing exposure time 45 min. However, at highest bacterial load 1×107 CFU ml− 1, > 78% bacterial load was removed by highest SPIONs concentration (1000 µM) by 15 min exposure, which was further increased > 85.32% by increasing the exposure time 60 min. The antibacterial activity was increased with increasing the concentration of SPIONs as well as exposure time while increasing the bacterial load the activity was decreases. At an initial bacterial load of E. tarda (1×103 to 1×107 CFU ml− 1), the effective concentration (EC50) ranged between 61.36 to 372.80 µM respectively after 15 to 60 min exposure. Thus, it can be concluded that the antibacterial activity of SPIONs depends on concentration and exposure time. To remove high bacterial load require high amount of SPIONs and exposure time as well. Hence, SPIONs can be used as a biocidal agent to treat Edwardsiellosis in fish.
Effective Virtualization based on Generalized Multi protocol Label Switching for Cloud Computing
Authors: rajat rajat saxena
Published: June 2022

Generalized Multi Protocol Label Switching (GMPLS) or Multi-Protocol Lambda Switching is a new technology which produce amplification in Multi Protocol Label Switching (MPLS) to provide support network switching, space switching, and packet switching for time and wavelength. Thus, we can say that GMPLS is extension of MPLS which provides resilience and restoration by automatic switching.In this paper, we provide a Docker based simulation environment which emulates a complex network and create insight functioning of GMPLS. This Docker based simulation environment is light-weight and scalable. It emulates large and complex network with minimal specifications. We have tested this Docker based GMPLS simulation testbed that depends on scaled network resources. Our method has shown tremendous improvement over the other virtualization methods.
Preliminary antimicrobial activity of CT-K3K7, a modified peptide by lysine substitutions from scorpion venom peptide
Authors: Li Shasha , Shui Yingbin , Ma Jiayue , Yuan Yaping , Jiang Wenxing , Xu Chenxi , Wang Lejing , Ren Yongjing , Deng Bo , Zhang Wenlu , Li Zhongjie
Published: June 2022

Antimicrobial peptides (AMPs) have gained interest as a new type of antimicrobial agent. Scorpion venom peptide ctry2459 were modified to CT-K3K7 by lysine substitutions at position 3rd and 7th. We found that the modified peptides CT-K3K7 with improved antibacterial activity, higher thermal stability and lower hemolytic activity. It can rapidly kill S. aureus and P. aeruginosa, reduce the production of biofilm and live bacteria residues in biofilm in vitro. CT-K3K7 has also been demonstrated that could decrease the bacterial counts in the subcutaneous abscess region, abscess area, and inflammatory cell infiltration in mouse subcutaneous abscess models duplicated by S. aureus and P. aeruginosa. CT-K3K7 is difficult to induce S. aureus and P. aeruginosa to develop drugs resistance, which may be related to the bactericidal way that the increase of cationic properties by lysine substitutions can increase the electrostatic force between the peptides and the bacterial surface that lead to the increase of bacterial membrane permeability and DNA binding. In conclusion, the modified peptide CT-K3K7 enhanced antimicrobial activity and can be as a new antimicrobial agent candidate for the treatment of infections by S. aureus and P. aeruginosa.
Use of ramucirumab for various treatment lines in real-world practice of patients with advanced hepatocellular carcinoma
Authors: Kanogawa Naoya , Ogasawara Sadahisa , Maruta Susumu , Iino Yotaro , Obu Masamichi , Ishino Takamasa , Ogawa Keita , Yumita Sae , Iwanaga Terunao , Unozawa Hidemi , Nakagawa Miyuki , Fujiwara Kisako , Sakuma Takafumi , Fujita Naoto , Kojima Ryuta , Kanzaki Hiroaki , Koroki Keisuke , Kobayashi Kazufumi , Inoue Masanori , Kiyono Soichiro , Nakamura Masato , Kondo Takayuki , Saito Tomoko , Nakagawa Ryo , Nakamoto Shingo , Muroyama Ryosuke , Chiba Tetsuhiro , Itobayashi Ei , Koma Yoshihiro , Azemoto Ryosaku , Kato Jun , Kato Naoya
Published: June 2022

Background: Ramucirumab was shown to be effective as a second-line treatment after sorafenib in patients with advanced hepatocellular carcinoma (HCC) with alpha-fetoprotein levels >400 ng/mL in a worldwide phase 3 trial. Ramucirumab is used in patients pretreated with various systemic therapies in clinical practice. We retrospectively examined the treatment outcomes of ramucirumab administered to advanced HCC patients after diverse systemic therapies.Methods: Data were collected from patients with advanced HCC who received ramucirumab at three institutions in Japan. Radiological assessments were determined according to both Response Evaluation Criteria in Solid Tumours (RECIST) version 1.1 and modified RECIST and the Common Terminology Criteria for Adverse Events version 5.0 was used to assess adverse events.Results: A total of 37 patients treated with ramucirumab between June 2019 and March 2021 were included in the study. Ramucirumab was administered as second, third, fourth, and fifth-line treatment in 13 (35.1%), 14 (37.8%), eight (21.6%), and two (5.4%) patients, respectively. Most patients (29.7%) who received ramucirumab as a second-line therapy were pretreated with lenvatinib. The median progression-free survival of patients treated with ramucirumab was 2.7 months (95% confidence interval, 1.6–7.3). There were no notable adverse events and no significant changes in albumin bilirubin score during ramucirumab treatment.Conclusion: Although ramucirumab is used for various lines of treatment other than second-line immediately after sorafenib, its safety and efficacy were not significantly different from the findings of the REACH-2 trial.
Assessing the Cytotoxicity of Tio2-x Nanoparticles With a Different Ti3+ (Ti2+)/Ti4+ Ratio
Authors: Prokopiuk Volodymyr , Yefimova Svetlana , Onishchenko Anatolii , Kapustnik Valeriy , Myasoedov Valeriy , Maksimchuk Pavel , Butov Dmytro , Bespalova Irina , Tkachenko Anton
Published: June 2022

Titanium dioxide (TiO2) nanoparticles are promising biomedical agents characterized by good biocompatibility. In this study, we explored the cytotoxicity of TiO2-x nanoparticles with a different Ti3+(Ti2+)/Ti4+ ratio and analyzed the effeciency of eryptosis indices as a tool in nanotoxicology. Methods. Two types of TiO2-x nanoparticles (5 nm) with various Ti3+ (Ti2+)/ Ti4+ ratios in the crystal lattice were synthesized. 1-TiO2-x nanoparticles contained 54% Ti4+, 38% Ti3+ and 8% Ti2+, while the relative amount of Ti4+ and Ti3+ in the crystal lattice of 2-TiO2-x nanoparticles was 63% and 37%, respectively. Cell viability and cell motility induced by TiO2-x nanoparticles were investigated on primary fibroblast cultures. Eryptosis modulation by the nanoparticles along with cell death mechanisms was studied on rat erythrocytes.Results. We report that both TiO2-x nanoparticles don’t decrease the viability of fibroblasts simultaneously stimulating cell migration. Data from in vitro studies on erythrocytes indicate that TiO2-x nanoparticles trigger eryptosis via ROS- (1-TiO2-x) and Ca2+-mediated mechanisms (both TiO2-x nanoparticles) suggesting that evaluation of eryptosis parameters is a more sensitive nanotoxicological approach for TiO2-x nanoparticles than cultured fibroblast assays.Conclusion. TiO2-x nanoparticles are characterized by low toxicity against fibroblasts, but they induce eryptosis, which is shown to be a promising tool for nanotoxicity screening. The Ti3+ (Ti2+)/ Ti4+ ratio at least partially determines the cytotoxicity mechanisms for TiO2-x nanoparticles.
Adipose-derived mesenchymal stem cells secreted EVs: a potential cell-free therapy for canine renal ischemia-reperfusion injury
Authors: Liu Hai-Feng , Fuhao Chen , Liyuan Huang , Jiahui Cao , Yifan Liu , Zhijun Zhong , Ziyao Zhou , Zhihua Ren , Suizhong Cao , Liuhong Shen , Guangneng Peng , Xiaoping Ma
Published: June 2022

Canine renal ischemia-reperfusion (IR) causes severe acute kidney injury characterized by renal dysfunction and inflammatory disorder. Adipose-derived mesenchymal stem cells (ADMSCs) and their extracellular vesicles (EVs) are pluripotent adult stem cells with promising therapy for IR, based on their potent anti-inflammatory and immunomodulatory properties. The aims of this study were to explore the therapeutic efficacy of ADMSC-EVs in canine renal IR injury. MSCs were isolated from inguinal fat tissue in dogs and their EVs were isolated from third-passage of ADMSC. ADMSCs were charactered by differentiation ability and surface makers. ADMSC-EVs were charactered by transmission electron microscopy, NTA and surface markers. Canine IR model was used with ADMSC-EVs administration to evaluated the therapeutic effect of inflammation, oxidate stress, mitochondrial damage and apoptosis. Renal IR injury leads to severe histopathological lesions and significantly increasing in biomarkers of renal function, inflammation, apoptosis; however, ADMSC more potently attenuated these lesions and inhibited apoptosis by reducing mitochondrial damage. The findings may provide a cell-free therapy for canine renal IR injury and reveal the mechanism involved.
In silico analysis of the Key Receptors of SARS-CoV-2: ACE2 and TMPRSS2 in Head and Neck Cancer
Authors: Mariappan Vignesh , JeanPierre Aashika Raagavi , Pillai Agieshkumar Balakrishna
Published: June 2022

Background
It is reported that cancer patients are more susceptible to SARS-CoV-2 infection due to immunocompromised immunity. To understand the long-term impact of SARS-CoV-2 in HNSC cancer, the current study is designed to assess the status of two vital host molecules (ACE2 & TMPRSS2) for the entry of virus in HNSC patients using computational methods.
Methodology
We used public databases such as TIMER, UALCAN, and GEPIA2 to assess the mRNA expression and association of ACE2 and TMPRSS2 in HNSC. cBioPortal was used to assess the genetic alterations in the ACE2 and TMPRSS2 protein sequences that are involved in HNSC development. The CTD database was also used to identify the genes associated with COVID-19 and HNSC. Finally, the PANTHER online platform was used to investigate the protein-protein interaction between COVID-19 and HNSC progression genes collected from the CTD database.
Results
A differential expression of ACE2 (downregulated) and TMPRSS2 (upregulated) was noted in HNSC those with human papilloma viral infections. A strong genetic alteration in the protein sequence of ACE2 and TMPRSS2 supports their significant role in HNSC disease progression. In addition, the protein-protein interaction network revealed that genes associated with COVID-19 and HNSC are strongly involved in binding activity, catalytic activity, and various cancer signaling pathways.
Conclusion
From our in silico analysis, we would like to conclude that ACE2 and TMPRSS2 are strongly associated with disease progression in both pathological conditions (HNSC and COVID-19). So, it could serve as potential biomarkers for the early prediction of disease outcomes in cancer patients with COVID-19 disease. Thus, further investigation is needed to evaluate the long-term impact of SARS-CoV-2 infection on HNSC patients.
Translation techniques of subtitle from english into indonesian in a movie: a case on literature
Authors: Saputra Nanda , Sulistyani Sulistyani , Fatmawati Endang , Herman Herman
Published: June 2022

The object of research: This study is aimed to categorize the types of translation techniques and the most prevalent translation techniques used in movie subtitles, because the phenomenon that frequently occurs shows that it has a tendency to use subtitles regardless of their disadvantages, so the translator requires appropriate translation techniques in order to transfer the message clearly from the source language into the target language

Investigated problem: The recent phenomenon in Indonesia shows that it has the tendency on using the subtitle regardless its drawback. As it is known, there is still the use of subtitles that are not of good quality and there is also the use of subtitles whose quality is much better based on the use appropriate translation techniques.

Methodology: To answer the research question, a descriptive qualitative research strategy was utilized, as well as content analysis, which focuses on the analysis of the sorts of translation approaches proposed by Molina and Albir. The researchers used movie and documentation as the research instrument. The data source of this research was the movie script “The Spongebob Movie: Sponge on The Run”

The area of practical use of the research results: As the result of this research, the researchers found 14 types of translation techniques. The most dominant of translation techniques is literal translation 67 data (33.5 %), followed by modulation 55 data (27.5 %), generalization 26 data (13 %), linguistic amplification 11 data (5.5 %), established equivalence 9 data (4.5 %), linguistic compression 8 data (4 %), transposition 7 data (3.5 %), compensation 5 data (2.5 %), calque and reduction 3 data for each other (1.5 %), borrowing and discursive creation 2 data for each other (1 %), adaptation and description 1 data for each other (0.5 %). Involving the total data are 200 data

Innovative technological product: The result of this research as the reflection and references for linguists and scientist not only to the quality of machine translation such as google translate, and so on but also to the use of good translation in the subtitle in the future.

Scope of the innovative technological product: The scope lies on the subtitle product produced by translator. The translator needs to know the target (audience) and the translation must meet the criteria and the target.

Conclusion: It can be concluded that the translation techniques used in the translation of subtitle are literal translation and modulation. The result shows that literal translation and modulation are the main contributors on the translation techniques in order to accurately translate it
Metagovernance and Democratic Legitimacy: a theoretical essay in light of non-market approaches
Authors: Andrade Guilherme Paulo , Andrade de Abreu Júlio César , Santos Ruan Carlos dos , Khatib Saleh Fadel Ahmad
Published: June 2022

Purpose of the study: The aim of this study is to demonstrate why metagovernance is not effective in increasing the democratic legitimacy of networks; to this end, an articulated map will be presented to describe how interactions occur within metagoverned environments.
Methodology/approach: To construct the macro-model of analysis, the methodology of Quivy and Campenhoudt (2005) was used. To consolidate the knowledge involved in the theoretical approach, the Knowledge Development Process-Constructivist (Proknow-C) method by Ensslin, Dutra and Ensslin (2000)was used. Such a proposal allowed a multidisciplinary perspective, capturing important concepts from economics and organizational theory to apply them to the meta-governed environment.
Originality/Relevance: Metagovernance systems suggest some ability to provide democratic plurality to a social environment, in that we need to understand "if" and "how" this applies; metagovernance is recent in academia and is under discussion; we need to understand its impact in countries with developing institutional environments.
Key findings: Metagoverned systems involve multiple players; difficulty of reconciliation raises transaction costs, both ex-ante - to achieve consensus in the network - and ex-post - when creating mechanisms to deal with disgruntled actors. Such a scenario leads key players to effect coalitions to 'narrow the road' to theirown interests, and practice corporate political activity seeking to influence decision making. Actions will focus on impacting the public agent designated as metagovernor, in order to influence possible changes in the environment (regulation).
Theoretical/methodological contributions: An articulated map has been proposed, presenting the cycle of interactions within a metagoverned environment. It shows that sometimes the decisions taken are not the most democratic ones, but those of interest to coalitions.
Social contributions / to management: From a legitimacy perspective, it is necessary to understand how the outcome of networked decision-making can become more democratic, and how metagoverned environments can become less susceptible to harmful corporate political activity, especially in developing democracies.
Glazbene preferencije mlađih adolescenata
Authors: Begić Amir , Šulentić Begić Jasna
Published: June 2022

Glazba ima važnu ulogu za adolescente jer se u tom životnom razdoblju oblikuju osobni i društveni identiteti, ali izgrađuju i glazbene preferencije. U okviru ovoga rada provedeno je online anketiranje koje je obuhvatilo 200 sudionika, odnosno učenika osnovnih općeobrazovnih škola iz pet hrvatskih županija u dobi od 13 i 14 godina. Cilj istraživanja bio je doznati glazbene preferencije učenika te utječu li sociodemografske varijable i nastava glazbe na njihove preferencije. Djevojčice su iskazale nešto veću sklonost prema svim ponuđenim vrstama glazbe, a posebice prema popularnoj glazbi. Sudionici čiji se roditelji bave glazbom značajno su skloniji umjetničkoj i popularnoj glazbi. Kada je u pitanju mjesto življenja, značajne razlike uočene su kod sklonosti rock glazbi koja je značajno viša kod sudionika iz grada. Sudionici koji procjenjuju da postoji utjecaj nastave glazbe i oni koji kod kuće slušaju skladbe s nastave značajno češće izvještavaju o interesu za umjetničku glazbu. Rezultati istraživanja ukazuju na potrebu većeg angažmana učitelja glazbe i roditelja u stjecanju glazbenih preferencija djece, posebice prema kvalitetnoj glazbi.
On the logarithmic bipartite fidelity of the open XXZ spin chain at $Δ=-1/2$
Authors: Hagendorf Christian , Parez Gilles
Published: June 2022

The open XXZ spin chain with the anisotropy
\Delta=-\frac12Δ=−12
and a one-parameter family of diagonal boundary fields is studied at
finite length. A determinant formula for an overlap involving the spin
chain’s ground-state vectors for different lengths is found. The overlap
allows one to obtain an exact finite-size formula for the ground state’s
logarithmic bipartite fidelity. The leading terms of its asymptotic
series for large chain lengths are evaluated. Their expressions confirm
the predictions of conformal field theory for the fidelity.
On the dynamic distinguishability of nodal quasi-particles in overdoped cuprates
Authors: Behnia Kamran
Published: June 2022

La_{1.67}1.67Sr_{0.33}0.33CuO_44
is not a superconductor and its resistivity follows a purely
T^22
temperature dependence at very low temperatures.
La_{1.71}1.71Sr_{0.29}0.29CuO_44,
on the other hand, has a superconducting ground state together with a
T-Linear term in its resistivity. The concomitant emergence of these two
features below a critical doping is mystifying. Here, I notice that the
electron-electron collision rate in the Fermi liquid above the doping
threshold is unusually large. The scattering time of nodal
quasi-particles expressed in a dimensionless parameter
\zetaζ
is very close to what has been found in liquid
^33He
at its melting pressure. In the latter case, fermionic particles become
dynamically distinguishable by excess of interaction. Ceasing to be
dynamically indistinguishable, nodal electrons will be excluded from the
Fermi sea. Such non-degenerate carriers will then scatter the degenerate
ones within a phase space growing linearly with temperature.
Патоморфологическая структура колоректального рака в Курской области в динамике в период с 2019-2021 год
Authors:   Кафедра онкологии Курского государственного медицинского университета, Быканова Анна ,   Курский онкологический научно-клинический центр им. Г.Е. Островерхова,   ФГБУ «Научный медицинский исследовательский центр эндокринологии» Минздрава России, Пирогов Андрей , Петроченко Дмитрий , Хвостовой Владимир , Станоевич Ирина , Станоевич Углеша
Published: June 2022

Оценить патоморфологическую структуру колоректального рака в Курской области в динамике в период с 2019-2021 г.г.
Возрастные особенности эластических волокон межпозвонкового диска L5S1 у человека
Authors:   Кафедра анатомии и оперативной хирургии, Южно-Уральский государственный медицинский университет, Куренков Евгений , Макарова Виктория ,   Кафедра анатомии и оперативной хирургии, Южно-Уральский государственный медицинский университет
Published: June 2022

Оценить количество эластических волокон в передней и задней зонах межпозвонкового диска L5S1 у лиц зрелого и пожилого возраста.
Этиология, патогенез, особенности симптомов и дифференциальной диагностики в клинике острого аппендицита и тифлита
Authors:   Воронежский базовый медицинский колледж, Малеев Юрий , Малюков Николай ,   ГБУЗ МКНЦ им. А.С. Логинова ДЗМ, Чередников Евгений ,   ГБОУ ВО «Воронежский государственный медицинский университет им. Н. Н. Бурденко» Минздрава России, Филин Андрей ,   ГБОУ ВО «Воронежский государственный медицинский университет им. Н. Н. Бурденко» Минздрава России, Баранников Сергей ,   ГБОУ ВО «Воронежский государственный медицинский университет им. Н. Н. Бурденко» Минздрава России, Банин Игорь ,   ГБОУ ВО «Воронежский государственный медицинский университет им. Н. Н. Бурденко» Минздрава России, Леонтьев Александр ,   ГБУЗ МКНЦ им. А.С. Логинова ДЗМ, Авилов Геннадий ,   ГБОУ ВО «Воронежский государственный медицинский университет им. Н. Н. Бурденко» Минздрава России
Published: June 2022

Выявить основные характеристические черты этиологии, патогенеза, особенностей симптомов и дифференциальной диагностики в клинике острого аппендицита (ОА) и тифлита
Гранулематозный полиангит и COVID-19. Клинико-морфологический анализ наблюдения
Authors:   НИИ морфологии человека им. академика А.П. Авцына, ФГБНУ «РНЦХ им. акад. Б.В. Петровского, Мнихович М. , Безуглова Т. ,   НИИ морфологии человека им. академика А.П. Авцына, ФГБНУ «РНЦХ им. акад. Б.В. Петровского, Романов А. ,   НИИ морфологии человека им. академика А.П. Авцына, ФГБНУ «РНЦХ им. акад. Б.В. Петровского, Лозина М. ,   ФГАОУ ВО РНИМУ Н. И. Пирогова, Ширипенко И. ,   ФГАОУ ВО РНИМУ Н. И. Пирогова, Сидорова О. ,   ФГАОУ ВО РНИМУ Н. И. Пирогова, Каминер Д. ,   ФГАОУ ВО РНИМУ Н. И. Пирогова, Снегур С. ,   ГБУ РО "Областная клиническая больница", Павлова Ю. ,   ГБУ РО "Областная клиническая больница"
Published: June 2022

Гранулематоз с полиангиитом (ГПА), представляет собой аутоиммунное заболевание, относящееся к группе системных васкулитов с преимущественным поражением сосудов среднего и малого калибра (капилляров, венул, артериол и мелких артерий), и входит в группу особых некротизирующих васкулитов, ассоциированных с гиперпродукцией антинейтрофильных цитолитических антител (АНЦА) специфичных к миелопероксидазе (МПО) или протеиназе-3 нейтрофилов.
Вклад курских гистологов в развитие эндокринологии в ХХ веке
Authors:   Кафедра гистологии, эмбриологии, цитологии, Курский государственный медицинский университет, Никишина Нина , Иванов Александр ,   Кафедра гистологии, эмбриологии, цитологии, Курский государственный медицинский университет, Пучков Вячеслав ,   Кафедра гистологии, эмбриологии, цитологии, Курский государственный медицинский университет
Published: June 2022

Учёные-гистологи Курского государственного медицинского института внесли значительный вклад в развитие учения о согласованной работе внутренних органов с помощью желез внутренней секреции. Целью статьи являлся обзору научных результатов в области эндокринологии, полученных учёными кафедры гистологии Курского государственного медицинского института.
Approximation of supremum and infimum processes as a stochastic approach to the providing of homeostasis
Authors:   Southern Federal University, Beliavsky Grigory I. , Danilova Natalia V. ,   Southern Federal University, Ougolnitsky Gennady A. ,   Southern Federal University
Published: June 2022

We consider the calculation of bounded functional of the trajectories of a stationary diffusion process. Since an analytical solution to this problem does not exist, it is necessary to use numerical methods. One possible direction for obtaining the numerical method is applying the Monte Carlo (MC) method. This involves reproducing the trajectory of a random process with subsequent averaging over the trajectories. To simplify the reproduction of the trajectory, the Girsanov transform is used in this paper. The main goal is to approximate the supremum and infimum processes, which allows us to more accurately compute the mathematical expectation of a function depending on the values of the supremum and infimum processes at the end of the time interval compared to the classical method. The method is based on randomly dividing the interval of the time axis by stopping times passages of the Wiener process, approximating the density to replace the measure, and using the MC method to calculate the expectation. One of the applications of the method is the task of keeping a random process in a given area the problem of homeostasis.
Two-stage network games modeling the Belt and Road Initiative
Authors:   St. Petersburg State University, Suna Ping , Parilina Elena M. ,   St. Petersburg State University, Gaob Hongwei ,   Qingdao University, School of Mathematics and Statistics
Published: June 2022

Inspired by the Belt and Road Initiative, we introduce a model of two-stage network games, when players first form a directed network (the first stage) and then they may reconsider the actions made at the first stage and choose controls to influence other players in a positive or negative way (the second stage). At both stages players get their payoffs. Considering a cooperative version of the game, we examine the problem of subgame network consistency and design an imputation distribution procedure as a new system of stage payments to guarantee the long-term cooperation. The Shapley value with exogenous directed graph constraint is used as a cooperative solution concept. Finally, we prove that a cooperative subgame is convex which ensures the non-emptiness of the core.
A problem of the equidistant deployment for discrete-time multiagent systems
Authors:   St. Petersburg State University, Aleksandrov Alexander Yu. , Arakelov Al’bert I. ,   St. Petersburg State University
Published: June 2022

This article explores a discrete-time multiagent system on a line. This requires the design of a control protocol providing equidistant agent deployment on a given segment of the line under the constraint that each agent receives information about distances to its neighbors via an auxiliary agent. An approach to the solution of the stated problem is developed. This proves that, under the proposed control protocol, neither communication delay nor switching of communication graph destroy convergence of agents to the equidistant distribution. The results of a numerical simulation confirming the obtained theoretical conclusions are presented.
The digenean complex life cycle: phylostratigraphy analysis of the molecular signatures
Authors: Nesterenko Maksim , Shchenkov Sergei , Denisova Sofia , Starunov Viktor
Published: June 2022

The parasitic flatworms from Digenea group have been the object of numerous in-depth studies for several centuries. The question of the evolutionary origin and transformation of the digenean complex life cycle remains relevant and open due to the biodiversity of these parasites and the absence of fossil records. However, modern technologies and analysis methods allow to get closer to understanding the molecular basis of both the realization of the cycle and its complication. In the present study, we have applied phylostratigraphy and evolutionary transcriptomics approaches to the available digenean genomic and transcriptomic data and built ancestral genomes models. The comparison results of Platyhelminthes and Digenea ancestor genome models made it possible to identify which genes were gained and duplicated in the possible genome of digenean ancestor. Based on the bioprocesses enrichment analysis results, we assumed that the change in the regulation of many processes, including embryogenesis, served as a basis for the complication of the ancestor life cycle. The evolutionary transcriptomics results obtained revealed the “youngest” and “oldest” life cycle stages of Fasciola gigantica, F. hepatica, Psilotrema simillimum, Schistosoma mansoni, Trichobilharzia regenti, and T. szidati. Our results can serve as a basis for a more in-depth study of the molecular signatures of life cycle stages and the evolution transformation of individual organ systems and stage-specific traits.
Long Noncoding RNAs in Human Cancer and Apoptosis
Authors: Erdoğan İpek , Sweef Osama , Akgül Bünyamin
Published: June 2022

Genome annotations have uncovered the production of at least one transcript from nearly all loci in the genome at some given time throughout the development. Surprisingly a big chunk of these transcripts does not code for proteins and are relatively long in size, thus called long noncoding RNAs (lncRNAs). Next- and third-generation sequencing technologies have amassed numerous lncRNAs expressed under different phenotypic conditions; yet many remain to be functionally characterized. LncRNAs regulate gene expression by functioning as scaffold, decoy, signaling, and guide molecules both at the transcriptional and post-transcriptional levels, interacting with different types of macromolecules such as proteins, DNA and RNA. Here we review the potential regulatory role of lncRNAs in apoptosis and cancer as some of these lncRNAs may have the diagnostic and therapeutic potential in cancer.
BDNF and its Role in the Alcohol Abuse Initiated During Early Adolescence: Evidence from Preclinical and Clinical Studies
Authors: Cutuli Debora , Sampedro-Piquero Piquero
Published: June 2022

Brain-derived neurotrophic factor (BDNF) is a crucial brain signaling protein that is integral to many signaling pathways. This neurotrophin has shown to be highly involved in brain plastic processes such as neurogenesis, synaptic plasticity, axonal growth, and neurotransmission, among others. In the first part of this review, we revise the role of BDNF in different neuroplastic processes within the central nervous system. On the other hand, its deficiency in key neural circuits is associated with the development of psychiatric disorders, including alcohol abuse disorder. Many people begin to drink alcohol during adolescence, and it seems that changes in BDNF are evident after the adolescent regularly consumes alcohol. Therefore, the second part of this manuscript addresses the involvement of BDNF during adolescent brain maturation and how this process can be negatively affected by alcohol abuse. Finally, we propose different BNDF enhancers, both behavioral and pharmacological, which should be considered in the treatment of problematic alcohol consumption initiated during the adolescence.