bluuebunny/crossref_metadata_2025 huggingface dataset:
Cyclophilin A/EMMPRIN Axis Is Involved in Pro-Fibrotic Processes Associated with Thoracic Aortic Aneurysm of Marfan Syndrome Patients
Authors: Perrucci Gianluca L. , Rurali Erica , Corlianò Maria , Balzo Maria , Piccoli Michela , Moschetta Donato , Pini Alessandro , Gaetano Raffaella , Antona Carlo , Egea Gustavo , Fischer Gunter , Malešević Miroslav , Alamanni Francesco , Cogliati Elisa , Paolin Adolfo , Pompilio Giulio , Nigro Patrizia
Published: January 2020

Background: Marfan syndrome (MFS) is a genetic disease, characterized by thoracic aortic aneurysm (TAA), which treatment is to date purely surgical. Understanding of novel molecular targets is mandatory to unveil effective pharmacological approaches. Cyclophilin A (CyPA) and its receptor EMMPRIN are associated with several cardiovascular diseases, including abdominal aortic aneurysm. Here, we envisioned the contribution of CyPA/EMMPRIN axis in MFS-related TAA. Methods: We obtained thoracic aortic samples from healthy controls (HC) and MFS patients’ aortas and then isolated vascular smooth muscle cells (VSMC) from the aortic wall. Results: our findings revealed that MFS aortic tissue samples isolated from the dilated zone of aorta showed higher expression levels of EMMPRIN vs. MFS non-dilated aorta and HC. Interestingly, angiotensin II significantly stimulated CyPA secretion in MFS-derived VSMC (MFS-VSMC). CyPA treatment on MFS-VSMC led to increased levels of EMMPRIN and other MFS-associated pro-fibrotic mediators, such as TGF-β1 and collagen I. These molecules were downregulated by in vitro treatment with CyPA inhibitor MM284. Our results suggest that CyPA/EMMPRIN axis is involved in MFS-related TAA development, since EMMPRIN is upregulated in the dilated zone of MFS patients’ TAA and the inhibition of its ligand, CyPA, downregulated EMMPRIN and MFS-related markers in MFS-VSMC. Conclusions: these insights suggest both a novel detrimental role for CyPA/EMMPRIN axis and its inhibition as a potential therapeutic strategy for MFS-related TAA treatment.
Increased O-GlcNAcylation of c-Myc Promotes Pre-B Cell Proliferation
Authors: Lee Da Hee , Kwon Na Eun , Lee Won-Ji , Lee Moo-Seung , Kim Doo-Jin , Kim Ji Hyung , Park Sung-Kyun
Published: January 2020

O-linked β-N-acetylglucosamine (O-GlcNAc) modification regulates the activity of hundreds of nucleocytoplasmic proteins involved in a wide variety of cellular processes, such as gene expression, signaling, and cell growth; however, the mechanism underlying the regulation of B cell development and function by O-GlcNAcylation remains largely unknown. Here, we demonstrate that changes in cellular O-GlcNAc levels significantly affected the growth of pre-B cells, which rapidly proliferate to allow expansion of functional clones that express successfully rearranged heavy chains at the pro-B stage during early B cell development. In our study, the overall O-GlcNAc levels in these proliferative pre-B cells, which are linked to the glucose uptake rate, were highly induced when compared with those in pro-B cells. Thus, pharmacologically, genetically, or nutritionally, inhibition of O-GlcNAcylation in pre-B cells markedly downregulated c-Myc expression, resulting in cell cycle arrest via blockade of cyclin expression. Importantly, the population of B cells after the pro-B cell stage in mouse bone marrow was severely impaired by the administration of an O-GlcNAc inhibitor. These results strongly suggest that O-GlcNAcylation-dependent expression of c-Myc represents a new regulatory component of pre-B cell proliferation, as well as a potential therapeutic target for the treatment of pre-B cell-derived leukemia.
Microstructure and Mechanical Properties of Carbon/Carbon Composites Infiltrated with Ti–6Al–4V Titanium Alloy
Authors: Ni Yuanlin , Luo Ruiying
Published: January 2020

In this work, chemical vapor infiltration (CVI) was combined with reactive melt infiltration (RMI) using Ti–6Al–4V titanium alloy powder to prepare Cf/C–TiC composites. The microstructure and composition of Cf/C–TiC composites were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The flexural properties of the composites were also analyzed. The results indicated that the Ti–6Al–4V titanium alloy infiltrated the Cf/C preform and reacted with the pyrolytic carbon (PyC) to form a TiC–VC and Al4C3 matrix, and no residual Ti, Al, or V was detected. Moreover, Al4C3 was concentrated and independently distributed, whereas Ti and V reacted with C to form a TiC–VC solid solution. The porosity was 6.75%, and the bulk density of Cf/C–TiC was 1.96 g/cm3. The flexural strength, flexural modulus, and failure strains were 256 ± 18 MPa, 89 ± 9 GPa, and 0.93 ± 0.13%, respectively. The work of fracture of the Cf/C–TiC composite was about 6.8 ± 0.38 KJ/m2. Due to the propagation and deflection of cracks, as well as debonding and fiber pullout, the Cf/C–TiC composite showed ductile fracture behavior.
Negative Effects of Diurnal Changes in Acidification and Hypoxia on Early-Life Stage Estuarine Fishes
Authors: Morrell Brooke K. , Gobler Christopher J.
Published: January 2020

Estuaries serve as important nursery habitats for various species of early-life stage fish, but can experience cooccurring acidification and hypoxia that can vary diurnally in intensity. This study examines the effects of acidification (pH 7.2–7.4) and hypoxia (dissolved oxygen (DO) ~ 2–4 mg L−1) as individual and combined stressors on four fitness metrics for three species of forage fish endemic to the U.S. East Coast: Menidia menidia, Menidia beryllina, and Cyprinodon variegatus. Additionally, the impacts of various durations of exposure to these two stressors was also assessed to explore the sensitivity threshold for larval fishes under environmentally-representative conditions. C. variegatus was resistant to chronic low pH, while M. menidia and M. beryllina experienced significantly reduced survival and hatch time, respectively. Exposure to hypoxia resulted in reduced hatch success of both Menidia species, as well as diminished survival of M. beryllina larvae. Diurnal exposure to low pH and low DO for 4 or 8 h did not alter survival of M. beryllina, although 8 or 12 h of daily exposure through the 10 days posthatch significantly depressed larval size. In contrast, M. menidia experienced significant declines in survival for all intervals of diel cycling hypoxia and acidification (4–12 h). Exposure to 12-h diurnal hypoxia generally elicited negative effects equal to, or of greater severity, than chronic exposure to low DO at the same levels despite significantly higher mean DO exposure concentrations. This evidences a substantial biological cost to adapting to changing DO levels, and implicates diurnal cycling of DO as a significant threat to fish larvae in estuaries. Larval responses to hypoxia, and to a lesser extent acidification, in this study on both continuous and diurnal timescales indicate that estuarine conditions throughout the spawning and postspawn periods could adversely affect stocks of these fish, with diverse implications for the remainder of the food web.
Anaplastic Thyroid Carcinoma Histologically Mimicking a Plasmacytoma
Authors: Mo Joshua H. , Tan Donald , Zhao Xiaohui S. , Tjoa Tjoson , Wang Beverly Y.
Published: January 2020

Anaplastic (undifferentiated) thyroid carcinoma (ATC) is a rare malignancy which may arise from transformation of a pre-existing differentiated carcinoma. We report the unique case where a lesion of thyroid origin presented with the histological features of mature plasma cells. Immunohistochemistry confirmed the lesion to be an anaplastic thyroid carcinoma arising from papillary thyroid carcinoma. A tumor mimicking a malignancy of a different cellular origin can lead clinicians to incorrect treatment approaches. Careful correlation with clinical details and knowledge of these unique presentations is important for reaching the correct diagnosis.
Implications of Assessing Student-Driven Projects: A Case Study of Possible Challenges and an Argument for Reflexivity
Authors: Pedersen Sofie , Hobye Mads
Published: January 2020

Employing student-driven project work in a higher education setting challenges not only the way in which we understand students’ learning and how we define the expected learning outcomes, it also challenges our ways of assessing students’ learning. This paper will address this question specifically and illustrate with a case that highlights some of the challenges that may arise in practice when assessing student-driven, problem-based projects. The case involved an assessment situation in which a discrepancy arose between the internal and external examiner in relation to what was valued. The discrepancy had consequences not only for the concrete assessment of students’ work, but also for the validity of the problem-based university pedagogy in general, and it raised the question of how to assess students’ work adequately. The research focus of this study was to explore the implications of assessing student-driven projects within a progressive approach to higher education teaching, along with the potential underlying issues. We found a need for clear assessment criteria while insisting on a space for students’ creativity and reflexivity as essential parts of a learning process. The paper thus makes a case for the notion of reflexivity as an assessment criterion to be integrated into learning objectives.
Electrochemical Properties of Nitrogen and Oxygen Doped Reduced Graphene Oxide
Authors: Hartmann Sean J. , Iurchenkova Anna A. , Kallio Tanja , Fedorovskaya Ekaterina O.
Published: January 2020

Carbon nanostructures are promising electrode materials for energy storage devices because of their unique physical and chemical properties. Modification of the surface improves the electrochemical properties of those materials because of the changes in morphology, diffusion properties, and inclusion of additional contributions to redox processes. Oxygen-containing functional groups and nitrogen doped into the carbon matrix significantly contribute to the electrochemical behavior of reduced graphite oxide (RGO). In this work, RGO was synthesized during hydrothermal treatment of graphite oxide with a hydrazine sulfate aqueous solution. Different amounts of hydrazine sulfate were used to synthesize RGO with different nitrogen contents in the structure, and the same synthesis conditions made it possible to obtain a material with a similar composition of oxygen-containing functional groups. The materials with different nitrogen concentrations and similar amounts of oxygen were compared as electrode materials for a supercapacitor and as a negative electrode material for a Li-ion battery. It was shown that the presence of oxygen-containing functional groups has the greatest influence on the behavior and efficiency of supercapacitor electrode materials, while nitrogen atoms embedded in the graphene lattice play the largest role in lithium intercalation.
Numerical Study of a Liquid Metal Oscillating inside a Pore in the Presence of Lorentz and Capillary Forces
Authors: Vlachomitrou Maria , Pelekasis Nikos
Published: January 2020

In order to ensure stable power exhaust and to protect the walls of fusion reactors, liquid metals that are fed to the wall surface through a capillary porous system (CPS) are considered as alternative plasma-facing components (PFCs). However, operational issues like drop ejection and plasma contamination may arise. In this study, the unsteady flow of a liquid metal inside a single pore of the CPS in the presence of Lorentz forces is investigated. A numerical solution is performed via the finite element methodology coupled with elliptic mesh generation. A critical magnetic number is found (Bondm = 4.5) below which the flow after a few oscillations reaches a steady state with mild rotational patterns. Above this threshold, the interface exhibits saturated oscillations. As the Lorentz force is further increased, Bondm > 5.8, a Rayleigh–Taylor instability develops as the interface is accelerated under the influence of the increased magnetic pressure and a finite time singularity is captured. It is conjectured that eventually, drop ejection will take place that will disrupt cohesion of the interface and contaminate the surrounding medium. Finally, the dynamic response of different operating fluids is investigated, e.g., gallium, and the stabilizing effect of increased electrical conductivity and surface tension is demonstrated.
Interleukin-1 Beta Neutralization Attenuates Traumatic Brain Injury-Induced Microglia Activation and Neuronal Changes in the Globus Pallidus
Authors: Ozen Ilknur , Ruscher Karsten , Nilsson Robert , Flygt Johanna , Clausen Fredrik , Marklund Niklas
Published: January 2020

Traumatic brain injury (TBI) increases the risk of delayed neurodegenerative processes, including Parkinson’s disease (PD). Interleukin-1beta (IL-1β), a key pro-inflammatory cytokine, may promote secondary injury development after TBI. Conversely, neutralizing IL-1β was found to improve functional recovery following experimental TBI. However, the mechanisms underlying the behavioral improvements observed by IL-1β neutralization are still poorly understood. The present study investigated the role of IL-1β on the microglia response and neuronal changes in the globus pallidus in response to diffuse TBI. Mice were subjected to sham injury or the central fluid percussion injury (cFPI) (a model of traumatic axonal injury), and were randomly administered an IL-1β neutralizing or a control antibody at 30 min post-injury. The animals were analyzed at 2, 7, or 14 days post-injury. When compared to controls, mice subjected to cFPI TBI had increased microglia activation and dopaminergic innervation in the globus pallidus, and a decreased number of parvalbumin (PV) positive interneurons in the globus pallidus. Neutralization of IL-1β attenuated the microglia activation, prevented the loss of PV+ interneurons and normalized dopaminergic fiber density in the globus pallidus of brain-injured animals. These findings argue for an important role for neuro-inflammation in the PD-like pathology observed in TBI.
Functional Magnetic Graphene Composites for Biosensing
Authors: Li Fan , Huang Yan , Huang Kai , Lin Jing , Huang Peng
Published: January 2020

Magnetic graphene composites (MGCs), which are composed of magnetic nanoparticles with graphene or its derivatives, played an important role in sensors development. Due to the enhanced electronic properties and the synergistic effect of magnetic nanomaterials and graphene, MGCs could be used to realize more efficient sensors such as chemical, biological, and electronic sensors, compared to their single component alone. In this review, we first reviewed the various routes for MGCs preparation. Then, sensors based on MGCs were discussed in different groups, including optical sensors, electrochemical sensors, and others. At the end of the paper, the challenges and opportunities for MGCs in sensors implementation are also discussed.
Increased Cardiovascular Risk Associated with Chemical Sensitivity to Perfluoro–Octanoic Acid: Role of Impaired Platelet Aggregation
Authors: De Toni Luca , Radu Claudia Maria , Sabovic Iva , Di Nisio Andrea , Dall’Acqua Stefano , Guidolin Diego , Spampinato Salvatore , Campello Elena , Simioni Paolo , Foresta Carlo
Published: January 2020

Perfluoro–alkyl substances (PFAS), particularly perfluoro–octanoic acid (PFOA), are persisting environmental chemicals showing bioaccumulation in human tissues. Recently, exposure to PFAS has been associated with increased prevalence of cardiovascular diseases (CVDs). However, a causal role of PFAS in atherosclerosis pathogenesis is under-investigated. Here, we investigated the effect of PFOA exposure on platelets’ function, a key player in atherosclerosis process. PFOA accumulation in platelets was evaluated by liquid chromatography-mass spectrometry. Changes in platelets’ membrane fluidity and activation after dose-dependent exposure to PFOA were evaluated by merocyanine 540 (MC540) and anti P-Selectin immune staining at flow cytometry, respectively. Intracellular calcium trafficking was analyzed with Fluo4M probe, time-lapse live imaging. Platelets’ aggregation state was also evaluated with Multiplate® aggregometry analyzer in 48 male subjects living in a specific area of the Veneto region with high PFAS environmental pollution, and compared with 30 low-exposure control subjects. Platelets’ membrane was the major target of PFOA, whose dose-dependent accumulation was associated in turn with increased membrane fluidity, as expected by a computational model; increased activation at resting condition; and both calcium uptake and aggregation upon activation. Finally, exposed subjects had higher serum and platelets levels of PFOA, together with increased aggregation parameters at Multiplate®, compared with controls. These data help to explain the emerging association between PFAS exposure and CVD.
Characterization and Analysis of the Skin Microbiota in Acne: Impact of Systemic Antibiotics
Authors: Park Seo-Yeon , Kim Hei Sung , Lee Se Hoon , Kim Sungjoo
Published: January 2020

Systemic antibiotics are extensively used to control moderate to severe acne. Hence, it is crucial to understand their impact on the skin microbiota, which is supposedly perturbed. The purpose of this study was to compare the makeup and diversity of the skin microbiota in acne patients before and after taking oral antibiotics. A longitudinal cohort study was performed on 20 participants with moderate to severe facial acne with no recent use of oral and topical antibiotics/retinoids. Patients were prescribed oral doxycycline, 100 mg, twice daily for six weeks. Skin areas on the cheek were sampled for 16S ribosomal RNA gene sequencing at baseline, and after six weeks of doxycycline treatment. Ten males and 10 females aged 11 to 44 years with a median Investigator’s Global Assessment score of 3 (moderate) were enrolled. At baseline, Cutibacterium acnes (formerly Propionibacterium acnes) was the most dominant species followed by Staphylococcus epidermidis. Acne severity showed a positive correlation with the abundance of Cutibacterium acnes. Across all subjects, antibiotic treatment reduced clinical acne grades and was associated with a 1.96-fold reduction in the relative abundance of Cutibacterium acnes (p = 0.01, 95% CI −22% to −3%). Marked changes were also identified in other bacterial species, such as Cutibacterium granulosum (formerly Propionibacterium granulosum), which increased by 4.46-fold (p = 0.02, 95% CI 0.004% to 0.9%) in the treated samples. In general, antibiotics administration was associated with an increase in bacterial diversity (alpha diversity). Principal coordinates analysis showed mild clustering of samples by patient (analysis of similarity, R = 0.135, p = 0.04) whereas there was scant clustering with treatment (ANOSIM, R = 0.005; p = 0.29). In conclusion, we found individuals with acne to have a unique microbial signature. Acne treatment with systemic antibiotics was associated with changes in the composition and diversity of skin microbiota, especially Cutibacterium acnes, which correlates with acne severity. Our study provides insight into the skin microbiota in acne and how it is modulated by systemic antibiotics.
Iodine Status, Thyroid Function, and Birthweight: A Complex Relationship in High-Risk Pregnancies
Authors: Velasco Inés , Sánchez-Gila Mar , Manzanares Sebastián , Taylor Peter , García-Fuentes Eduardo
Published: January 2020

(1) Background: The consequences of iodine deficiency and/or thyroid dysfunction during pregnancy have been extensively studied, emphasizing on infant neurodevelopment. However, the available information about the relationship between iodine, thyroid hormones, and fetal growth in high-risk pregnancies is limited. We aim to investigate if iodine metabolism and/or thyroid parameters can be affected by adverse antenatal/perinatal conditions. (2) Methods: A cross-sectional study examined differences in iodine status, thyroid function, and birthweight between high-risk (HR group; n = 108)) and low-risk pregnancies (LR group; n = 233) at the time of birth. Urinary iodine concentration (UIC), iodine levels in amniotic fluid, and thyroid parameters [thyroid-stimulating hormone (TSH), free thyroxine (FT4)] were measured in mother–baby pairs. (3) Results: There were significant differences between HR and LR groups, free thyroxine (FT4) concentration in cord blood was significantly higher in the LR group compared with HR pregnancies (17.06 pmol/L vs. 15.30 pmol/L, respectively; p < 0.001), meanwhile iodine concentration in amniotic fluid was significantly lower (13.11 µg/L vs. 19.65 µg/L, respectively; p < 0.001). (4) Conclusions: Our findings support the hypothesis that an adverse intrauterine environment can compromise the availability of FT4 in cord blood as well as the iodine metabolism in the fetus. These differences are more noticeable in preterm and/or small fetuses.
Threshold Voltage Degradation for n-Channel 4H-SiC Power MOSFETs
Authors: Guevara Esteban , Herrera-Pérez Victor , Rocha Cristian , Guerrero Katherine
Published: January 2020

In this study, threshold voltage instability on commercial silicon carbide (SiC) power metal oxide semiconductor field electric transistor MOSFETs was evaluated using devices manufactured from two different manufacturers. The characterization process included PBTI (positive bias temperature instability) and pulsed IV measurements of devices to determine electrical parameters’ degradations. This work proposes an experimental procedure to characterize silicon carbide (SiC) power MOSFETs following two characterization methods: (1) Using the one spot drop down (OSDD) measurement technique to assess the threshold voltage explains temperature dependence when used on devices while they are subjected to high temperatures and different gate voltage stresses. (2) Measurement data processing to obtain hysteresis characteristics variation and the damage effect over threshold voltage. Finally, based on the results, it was concluded that trapping charge does not cause damage on commercial devices due to reduced value of recovery voltage, when a negative small voltage is applied over a long stress time. The motivation of this research was to estimate the impact and importance of the bias temperature instability for the application fields of SiC power n-MOSFETs. The importance of this study lies in the identification of the aforementioned behavior where SiC power n-MOSFETs work together with complementary MOS (CMOS) circuits.
Synthesis and Characterization of PLA-Micro-structured Hydroxyapatite Composite Films
Authors: Pandele Andreea Madalina , Constantinescu Andreea , Radu Ionut Cristian , Miculescu Florin , Ioan Voicu Stefan , Ciocan Lucian Toma
Published: January 2020

This article presents a facile synthesis method used to obtain new composite films based on polylactic acid and micro-structured hydroxyapatite particles. The composite films were synthesized starting from a polymeric solution in chloroform (12 wt.%) in which various concentrations of hydroxyapatite (1, 2, and 4 wt.% related to polymer) were homogenously dispersed using ultrasonication followed by solvent evaporation. The synthesized composite films were morphologically (through SEM and atomic force microscopy (AFM)) and structurally (through FT-IR and Raman spectroscopy) characterized. The thermal behavior of the composite films was also determined. The SEM and AFM analyses showed the presence of micro-structured hydroxyapatite particles in the film’s structure, as well as changes in the surface morphology. There was a significant decrease in the crystallinity of the composite films compared to the pure polymer, this being explained by a decrease in the arrangement of the polymer chains and a concurrent increase in the degree of their clutter. The presence of hydroxyapatite crystals did not have a significant influence on the degradation temperature of the composite film.
Hydrogen Production from the LOHC Perhydro-Dibenzyl-Toluene and Purification Using a 5 µm PdAg-Membrane in a Coupled Microstructured System
Authors: Wunsch Alexander , Berg Tatjana , Pfeifer Peter
Published: January 2020

Hydrogen bound in organic liquid hydrogen carriers (LOHC) such as dibenzyl-toluene enables simple and safe handling as well as long-term storage. This idea is particularly interesting in the context of the energy transition, where hydrogen is considered a key energy carrier. The LOHC technology serves as a storage between volatile energy and locally and timely independent consumption. Depending on the type of application, decisive specifications are placed on the hydrogen purity. In the product gas from dehydrogenation, however, concentrations of 100 to a few 1000 ppm can be found from low boiling substances, which partly originate from the production of the LOHC material, but also from the decomposition and evaporation of the LOHC molecules in the course of the enormous volume expansion due to hydrogen release. For the removal of undesired traces in the LOHC material, a pre-treatment and storage under protective gas is necessary. For purification, the use of Pd-based membranes might be useful, which makes these steps less important or even redundant. Heat supply and phase contacting of the liquid LOHC and catalyst is also crucial for the process. Within the contribution, the first results from a coupled microstructured system—consisting of a radial flow reactor unit and membrane separation unit—are shown. In a first step, the 5 µm thick PdAg-membrane was characterized and a high Sieverts exponent of 0.9 was determined, indicating adsorption/desorption driven permeation. It can be demonstrated that hydrogen is first released with high catalyst-related productivity in the reactor system and afterwards separated and purified. Within the framework of limited analytics, we found that by using a Pd-based membrane, a quality of 5.0 (99.999% purity) or higher can be achieved. Furthermore, it was found that after only 8 hours, the membrane can lose up to 30% of its performance when exposed to the slightly contaminated product gas from the dehydrogenation process. However, the separation efficiency can almost completely be restored by the treatment with pure hydrogen.
Properties of FeAlSi-X-Y Alloys (X,Y=Ni, Mo) Prepared by Mechanical Alloying and Spark Plasma Sintering
Authors: Průša Filip , Proshchenko Olga , Školáková Andrea , Kučera Vojtěch , Laufek František
Published: January 2020

Short-term mechanical alloying and compaction by spark plasma sintering was used for the production of FeAl20Si20Mo20-XNiX (X corresponds to 5–15 wt %) alloy, which showed an ultrafine-grained microstructure with dimensions of phases around 200 nm or smaller. It was found that the addition of Mo and Ni to the FeAl20Si20 alloy results in the formation of the AlMoSi phase compared to the three-phase FeAl20Si20 alloy, which initially contained FeSi, Fe3Si, and Fe3Al2Si3 phases. All the investigated alloys increased their hardness, reaching up to 1401 HV 1 for the FeAl20Si20Mo5Ni15 alloy, which contained in total 58.5% of the FeSi and Fe3Al2Si3 phases. As a result, all the prepared alloys showed one order magnitude lower wear rates ranging from 3.14 to 5.97·10−6 mm3·N−1·m−1 as well as significantly lower friction coefficients compared to two reference tool steels. The alloys achieved high compressive strengths (up to 2200 MPa); however, they also exhibited high brittleness even after long-term annealing, which reduced the strengths of all the alloys below approximately 1600 MPa. Furthermore, the alloys were showing ductile behavior when compressively tested at elevated temperature of 800 °C. The oxidation resistance of the alloys was superior due to the formation of a compact Al2O3 protective layer that did not delaminate.
Non-Epsilon Dominated Evolutionary Algorithm for the Set of Approximate Solutions
Authors: Hernández Castellanos Carlos Ignacio , Schütze Oliver , Sun Jian-Qiao , Ober-Blöbaum Sina
Published: January 2020

In this paper, we present a novel evolutionary algorithm for the computation of approximate solutions for multi-objective optimization problems. These solutions are of particular interest to the decision-maker as backup solutions since they can provide solutions with similar quality but in different regions of the decision space. The novel algorithm uses a subpopulation approach to put pressure towards the Pareto front while exploring promissory areas for approximate solutions. Furthermore, the algorithm uses an external archiver to maintain a suitable representation in both decision and objective space. The novel algorithm is capable of computing an approximation of the set of interest with good quality in terms of the averaged Hausdorff distance. We underline the statements on some academic problems from literature and an application in non-uniform beams.
The Generation of Indole-2,3-quinodimethanes from the Deamination of 1,2,3,4-Tetrahydropyrrolo[3,4-b]indoles
Authors: Rinderspacher Alison , Gribble Gordon W.
Published: January 2020

A novel generation of indole-2,3-quinodimethanes via the deamination of 1,2,3,4-tetrahydropyrrolo[s3,4-b]indoles is reported.
Estimation of the Minimum Effective Dose of Dietary Supplement Crocetin for Prevention of Myopia Progression in Mice
Authors: Mori Kiwako , Kurihara Toshihide , Jiang Xiaoyan , Ikeda Shin-ichi , Yotsukura Erisa , Torii Hidemasa , Tsubota Kazuo
Published: January 2020

The natural carotenoid crocetin has been reported to suppress phenotypes of an experimental myopia model in mice. We investigated the minimum effective dose to prevent myopia progression in a murine model. Three-week-old male mice (C57B6/J) were equipped with a −30 diopter (D) lens to induce myopia, and fed with normal chow, 0.0003%, or 0.001% of crocetin-containing chow. Changes in refractive errors and axial lengths (AL) were evaluated after three weeks. Pharmacokinetics of crocetin in the plasma and the eyeballs of mice was evaluated with specific high sensitivity quantitative analysis using liquid chromatography tandem mass spectrometry (LC-MS/MS) to determine the minimum effective dosage. A concentration of 0.001% of crocetin-containing chow showed a significant (p < 0.001) suppressive effect against both refractive and AL changes in the murine model. Meanwhile, there was no significant difference of AL change between the 0.0003% and the normal chow groups. The concentration of crocetin in the plasma and the eyeballs from mice fed with 0.001% crocetin-containing chow was significantly higher than control and 0.0003% crocetin-containing chow. In conclusion, we suggest 0.001% of crocetin-containing extract is the minimum effective dose showing a significant suppressive effect against both refractive and AL changes in the murine model.
Arbuscular Mycorrhizal Fungi Mediate Drought Tolerance and Recovery in Two Contrasting Carob (Ceratonia siliqua L.) Ecotypes by Regulating Stomatal, Water Relations, and (In)Organic Adjustments
Authors: Boutasknit Abderrahim , Baslam Marouane , Ait-El-Mokhtar Mohamed , Anli Mohamed , Ben-Laouane Raja , Douira Allal , El Modafar Cherkaoui , Mitsui Toshiaki , Wahbi Said , Meddich Abdelilah
Published: January 2020

Irregular precipitation and drought caused an increase in tree mortality rates in multiple forest biomes with alterations in both ecosystem services and carbon balance. Carob (Ceratonia siliqua) growth and production in arid and semi-arid ecosystems are likely affected by climate change-induced droughts. Understanding the physiological responses of drought-induced early-stage tree death and strategies to enhance drought tolerance and optimize growth will help tree improvement programs. Mycorrhizal inoculation has a pronounced impact on plant growth, water absorption, mineral nutrition, and protection from abiotic stresses. However, a better understanding of these complex interconnected cellular processes and arbuscular mycorrhizal fungi (AMF)-mediated mechanisms regulating drought tolerance in plants will enhance its potential application as an efficient approach for bio-amelioration of stresses. The objectives of this work were to elucidate the different effects of autochthone AMF on inorganic solute and water content uptakes, organic adjustments (sugar and proteins content), leaf gas exchange (stomatal conductance and efficiency of photosystems I and II), and oxidative damage of two contrasting ecotypes of carob seedlings: coastal (southern ecotype (SE)) and in-land (northern ecotype (NE)) under control (C), drought (by cessation of irrigation for 15 days (15D)), and recovery (R) conditions. Our findings showed that AMF promoted growth, nutrient content, and physiological and biochemical parameters in plants of both ecotypes during C, 15D, and R conditions. After four days of recovery, stomatal conductance (gs), the maximum photochemical efficiency of PSII (Fv/Fm), water content, and plant uptake of mineral nutrients (P, K, Na, and Ca) were significantly higher in shoots of mycorrhizal (AM) than non-mycorrhizal (NM) control plants. Consequently, AMF reduced to a greater degree the accumulation of hydrogen peroxide (H2O2) and oxidative damage to lipid (malondialdehyde (MDA)) content in AM than NM plants in NE and SE, after recovery. Altogether, our findings suggest that AMF can play a role in drought resistance of carob trees at an early stage by increasing the inorganic solutes (P, K, Na, and Ca), water content uptake, organic solutes (soluble sugars and protein content), stomatal conductance, and defense response against oxidative damage during re-watering after drought stress.
The Ministering Critic: Kierkegaard’s Theology of Communication
Authors: Johnson Russell
Published: January 2020

This paper analyzes Kierkegaard’s scattered writings on communication to foreground the distinctively theological dimension of Kierkegaard’s rhetorical theory. “Indirect communication” needs to be understood as a strategy to address a specific theological problem, namely, the tendency for readers who think they are already Christian to dismiss or domesticate rhetoric that summons them to authentic Christian existence. Since Christianity is an “existence-communication,” the questions of what it means to be a Christian and how one can faithfully communicate Christianity are integrally linked for Kierkegaard. Contemporary apologists, activists, and preachers who rely on more direct modes of communication to express the Christian gospel have much to learn from Kierkegaard’s grappling with the illusions that beset Christian witness.
Field-Validated Burn-Severity Mapping in North Patagonian Forests
Authors: Franco María Guadalupe , Mundo Ignacio A. , Veblen Thomas T.
Published: January 2020

Burn severity, which can be reliably estimated by validated spectral indices, is a key element for understanding ecosystem dynamics and informing management strategies. However, in North Patagonian forests, where wildfires are a major disturbance agent, studies aimed at the field validation of spectral indices of burn severity are scarce. The aim of this work was to develop a field validated methodology for burn-severity mapping by studying two large fires that burned in the summer of 2013–2014 in forests of Araucaria araucana and other tree species. We explored the relation between widely used spectral indices and a field burn-severity index, and we evaluated index performance by examining index sensitivity in discriminating burn-severity classes in different vegetation types. For those indices that proved to be suitable, we adjusted the class thresholds and constructed confusion matrices to assess their accuracy. Burn severity maps of the studied fires were generated using the two most accurate methods and were compared to evaluate their level of agreement. Our results confirm that reliable burn severity estimates can be derived from spectral indices for these forests. Two severity indices, the delta normalized burn ratio (dNBR) and delta normalized difference vegetation index (dNDVI), were highly related to the fire-induced changes observed in the field, but the strength of these associations varied across the five different vegetation types defined by tree heights and tree and tall shrub species regeneration strategies. The thresholds proposed in this study for these indices generated classifications with global accuracies of 82% and Kappa indices of 70%. Both the dNBR and dNDVI classification approaches were more accurate in detecting high severity, but to a lesser degree for detecting low severity burns. Moderate severity was poorly classified, with producer and user errors reaching 50%. These constraints, along with detected differences in separability, need to be considered when interpreting burn severity maps generated using these methods.
An End-To-End Bayesian Segmentation Network Based on a Generative Adversarial Network for Remote Sensing Images
Authors: Xiong Dehui , He Chu , Liu Xinlong , Liao Mingsheng
Published: January 2020

Due to the development of deep convolutional neural networks (CNNs), great progress has been made in semantic segmentation recently. In this paper, we present an end-to-end Bayesian segmentation network based on generative adversarial networks (GANs) for remote sensing images. First, fully convolutional networks (FCNs) and GANs are utilized to realize the derivation of the prior probability and the likelihood to the posterior probability in Bayesian theory. Second, the cross-entropy loss in the FCN serves as an a priori to guide the training of the GAN, so as to avoid the problem of mode collapse during the training process. Third, the generator of the GAN is used as a teachable spatial filter to construct the spatial relationship between each label. Some experiments were performed on two remote sensing datasets, and the results demonstrate that the training of the proposed method is more stable than other GAN based models. The average accuracy and mean intersection (MIoU) of the two datasets were 0.0465 and 0.0821, and 0.0772 and 0.1708 higher than FCN, respectively.
Direct Estimation of Forest Leaf Area Index based on Spectrally Corrected Airborne LiDAR Pulse Penetration Ratio
Authors: Qu Yonghua , Shaker Ahmed , Korhonen Lauri , Silva Carlos Alberto , Jia Kun , Tian Luo , Song Jinling
Published: January 2020

The leaf area index (LAI) is a crucial structural parameter of forest canopies. Light Detection and Ranging (LiDAR) provides an alternative to passive optical sensors in the estimation of LAI from remotely sensed data. However, LiDAR-based LAI estimation typically relies on empirical models, and such methods can only be applied when the field-based LAI data are available. Compared with an empirical model, a physically-based model—e.g., the Beer–Lambert law based light extinction model—is more attractive due to its independent dataset with training. However, two challenges are encountered when applying the physically-based model to estimate LAI from discrete LiDAR data: i.e., deriving the gap fraction and the extinction coefficient from the LiDAR data. We solved the first problem by integrating LiDAR and hyperspectral data to transfer the LiDAR penetration ratio to the forest gap fraction. For the second problem, the extinction coefficient was estimated from tiled (1 km × 1 km) LiDAR data by nonlinearly optimizing the cost function of the angular LiDAR gap fraction and simulated gap fraction from the Beer–Lambert law model. A validation against LAI-2000 measurements showed that the estimates were significantly correlated to the reference LAI with an R2 of 0.66, a root mean square error (RMSE) of 0.60 and a relative RMSE of 0.15. We conclude that forest LAI can be directly estimated by the nonlinear optimization method utilizing the Beer–Lambert model and a spectrally corrected LiDAR penetration ratio. The significance of the proposed method is that it can produce reliable remotely sensed forest LAI from discrete LiDAR and spectral data when field-measured LAI are unavailable.
Cognitive Interference Cancellation with Digital Channelizer for Satellite Communication
Authors: Kim Byounghak , Yu Heejung , Noh Song
Published: January 2020

The concept of Internet of Things (IoT) has attracted much research attention for the realization of a smart society. However, the radio transmission coverage of the existing IoT solutions is not enough to connect lots of devices deployed over wide areas. Therefore, satellite networks have been considered as one of the most attractive solutions to wide cell coverage of IoT, i.e., global-scaled IoT. In satellite communication, a digital channelizer is one of the most significant parts that support multiple transponders. Owing to their wide coverage, satellite communication systems are more vulnerable to interference than other types of wireless communication systems. In this study, a cognitive interference cancellation using the inherent properties of a digital channelizer is considered. The proposed method detects a subchannel corrupted by interference and omits it. A simple energy detection method and a modified version are proposed for detection of interference. In the modified (i.e., improved) method, the number of required signal blocks to achieve the target detection performance can be reduced, i.e., the detection performance is improved with the same number of blocks, by exploiting the property of the fast Fourier transform (FFT) algorithm. Detection performance such as false alarm and detection probabilities are analyzed, and the validity of the analysis is verified with numerical results. It is also shown that an interference lower than a certain level in the proposed approach does not need to be cancelled.
Developing a Silk Fibroin Composite Film to Scavenge and Probe H2O2 Associated with UV-Excitable Blue Fluorescence
Authors: Chung Tze-Wen , Chang Chun-Yi , Chang Chun-Ning , Liao Chiu-Hsun , Jan Yun-Jen , Chen Li-Ting , Chen Weng-Pin
Published: January 2020

A silk fibroin composite film that can simultaneously scavenge and probe H2O2 in situ was developed for possibly examining local concentrations of H2O2 for biomedical applications. A multi-functional composite film (GDES) that consists of graphene oxide (G), a photothermally responsive element that was blended with polydopamine (PDA, D)/horseradish peroxidase (HRP, E) (or DE complex), and then GDE microaggregates were coated with silk fibroin (SF, S), a tyrosine-containing protein. At 37 °C, the H2O2-scavenging ability of a GDES film in solution at approximately 7.5 × 10−3 μmol H2O2/mg film was the highest compared with those of S and GS films. The intensities of UV-excitable blue fluorescence of a GDES film linearly increased with increasing H2O2 concentrations from 4.0 μM to 80 μM at 37 °C. Interestingly, after a GDES film scavenged H2O2, the UV-excitable blue fluorescent film could be qualitatively monitored by eye, making the film an eye-probe H2O2 sensor. A GDES film enabled to heat H2O2-containing samples to 37 °C or higher by the absorption of near-IR irradiation at 808 nm. The good biocompatibility of a GDES film was examined according to the requirements of ISO-10993-5. Accordingly, a GDES film was developed herein to scavenge and eye-probe H2O2 in situ and so it has potential for biomedical applications.
Interference Mitigation for Visible Light Communications in Underground Mines Using Angle Diversity Receivers
Authors: Palacios Játiva Pablo , Román Cañizares Milton , Azurdia-Meza Cesar A. , Zabala-Blanco David , Dehghan Firoozabadi Ali , Seguel Fabian , Montejo-Sánchez Samuel , Soto Ismael
Published: January 2020

This paper proposes two solutions based on angle diversity receivers (ADRs) to mitigate inter-cell interference (ICI) in underground mining visible light communication (VLC) systems, one of them is a novel approach. A realistic VLC system based on two underground mining scenarios, termed as mining roadway and mine working face, is developed and modeled. A channel model based on the direct component in line-of-sight (LoS) and reflections of non-line-of-sight (NLoS) links is considered, as well as thermal and shot noises. The design and mathematical models of a pyramid distribution and a new hemi-dodecahedral distribution are addressed in detail. The performances of these approaches, accompanied by signal combining schemes, are evaluated with the baseline of a single photo-diode in reception. Results show that the minimum lighting standards established in both scenarios are met. As expected, the root-mean-square delay spread decreases as the distance between the transmitters and receivers increases. Furthermore, the hemi-dodecahedron ADR in conjunction with the maximum ratio combining (MRC) scheme, presents the best performance in the evaluated VLC system, with a maximum user data rate of 250 Mbps in mining roadway and 120 Mbps in mine working face, received energy per bit/noise power of 32 dB and 23 dB, respectively, when the bit error rate corresponds to 10 − 4 , and finally, values of 120 dB in mining roadway and 118 dB in mine working face for signal-to-interference-plus-noise ratio are observed in a cumulative distribution function.
Effect of N-Amide Substitution on Antioxidative Activities of Melatonin Derivatives
Authors: Panyatip Panyada , Pratheepawanit Johns Nutjaree , Priprem Aroonsri , Nakagawa Kouichi , Puthongking Ploenthip
Published: January 2020

Five N-amide substituted melatonin (MLT) derivatives were synthesized and evaluated for antioxidative activities, and compounds 9–12 showed higher electron spin resonance (ESR) response than MLT. 4-Bromobenzoyl and naphthoyl derivatives (10 and 11) presented stronger hydroxyl radical inhibitory effect than MLT in Fenton reaction. The substitution at the N1-position on the MLT core structure with acetyl (8), benzoyl (9), 4-bromobenzoyl (10), and naphthoyl (11) and N2-substitution with 4-bromobenzoyl (12) decreased the reducing power of the derivatives in ferric reducing antioxidant power (FRAP) assay. Compounds 8–11 also presented lower antioxidant capacity than their parent compound in 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) disodium salt (ABTS) assay; whereas, compound 12 presented radical scavenging activity similarly to MLT. All aryl derivatives (9–12) showed higher ability to quench peroxyl radicals than MLT about three times, especially the benzoylated derivatives (9 and 10) that presented the highest ability in oxygen radical absorbance capacity (ORAC) assay.
Exploring the Spatio-Temporal Dynamics of Winter Rape on the Middle Reaches of Yangtze River Valley Using Time-Series MODIS Data
Authors: Tao Jianbin , Wu Wenbin , Liu Wenbin , Xu Meng
Published: January 2020

Rapeseed is an important oil product in China. China’s current soybean trade issues with major soybean producing countries have caused a large decline in soybean import since 2017. This may bring the increasing needs of rapeseed import, which would have an impact on domestic production. However, our knowledge on the effects of international rapeseed trade on domestic production remains unknown. It is thus important to understand the pattern of rapeseed in China under this scenario, as it may provide necessary information for all relevant stakeholders. With this goal, this study aims to investigate the spatial and temporal patterns of winter rape in China’s major winter rape production region, the Middle Reaches of the Yangtze River Valley (MYR), during 2003–2015 using time-series Moderate Resolution Imaging Spectroradiometer (MODIS) data. A decision tree according to the difference in enhanced vegetation index (EVI) profiles of land-cover types was built to extract winter rape. The results show that there is an essential decrease in both the number and density of winter rape patches under the opening global rapeseed market. There are significant hotspots of winter rape gain and loss, within which the loss dominated the trend. The significant cost advantage of rapeseed in the international market may largely reduce the domestic cultivation in China through telecoupling effects. Understanding the spatio-temporal dynamics of winter rape on the MYR has significant economic and policy implications and can provide great supports for the agricultural production, policy-making, and oil products trade in the international market.