bluuebunny/crossref_metadata_2025 huggingface dataset:
Remodeling of Hepatocyte Mitochondrial Metabolism and De Novo Lipogenesis During the Embryonic-to-Neonatal Transition in Chickens
Authors: Surugihalli Chaitra , Farley Linda S. , Beckford Ronique C. , Kamkrathok Boonyarit , Liu Hsiao-Ching , Muralidaran Vaishna , Patel Kruti , Porter Tom E. , Sunny Nishanth E.
Published: April 2022

Embryonic-to-neonatal development in chicken is characterized by high rates of lipid oxidation in the late-term embryonic liver and high rates of de novo lipogenesis in the neonatal liver. This rapid remodeling of hepatic mitochondrial and cytoplasmic networks occurs without symptoms of hepatocellular stress. Our objective was to characterize the metabolic phenotype of the embryonic and neonatal liver and explore whether these metabolic signatures are preserved in primary cultured hepatocytes. Plasma and liver metabolites were profiled using mass spectrometry based metabolomics on embryonic day 18 (ed18) and neonatal day 3 (nd3). Hepatocytes from ed18 and nd3 were isolated and cultured, and treated with insulin, glucagon, growth hormone and corticosterone to define hormonal responsiveness and determine their impacts on mitochondrial metabolism and lipogenesis. Metabolic profiling illustrated the clear transition from the embryonic liver relying on lipid oxidation to the neonatal liver upregulating de novo lipogenesis. This metabolic phenotype was conserved in the isolated hepatocytes from the embryos and the neonates. Cultured hepatocytes from the neonatal liver also maintained a robust response to insulin and glucagon, as evidenced by their contradictory effects on lipid oxidation and lipogenesis. In summary, primary hepatocytes from the embryonic and neonatal chicken could be a valuable tool to investigate mechanisms regulating hepatic mitochondrial metabolism and de novo lipogenesis.
Luminescence of Agrotextiles Based on Red-Light-Emitting Organic Luminophore and Polypropylene Spunbond Enhances the Growth and Photosynthesis of Vegetable Plants
Authors: Khramov Robert , Kosobryukhov Anatoly , Kreslavski Vladimir , Balakirev Dmitry , Khudyakova Alexandra , Svidchenko Evgeniya , Surin Nikolay , Ponomarenko Sergey , Luponosov Yuriy
Published: April 2022

The impact of a light-transforming covering on photosynthetic activity and growth processes in lettuce and white cabbage plants grown in a glass greenhouse was studied. Plants were covered with agrotextile, a polypropylene (PP) nonwoven spunbond coated with polylactide varnish containing a new organic luminophore (LUM), which absorbs sunlight mainly in the 460–560 nm region and efficiently reradiates it in the red spectral region with a maximum at 660 nm. For comparison, simultaneously two references agrotextiles without LUM or containing a non-luminescent chromophore (ABS) with an absorption spectrum close to that of LUM were as well investigated. The use of the agrotextile with LUM resulted in a significant increase in total crude aboveground biomass for 32-, 33-, and 43-day-old plants on the average by 20–40%, and the photosynthesis rate increased on the average by 30–40% compared to the agrotextile without LUM. The use of the agrotextile with ABS mimicking the absorption of LUM also did not reveal a significant impact on photosynthesis and biomass accumulation in the plants as compared to the reference agrotextile coated only with the polylactide varnish. At the same time, the photosystem II activity (Fv/Fm and F′v/F′m quantum yields) was nearly the same in all experiments. When plants were grown under the light-converting agrotextile, the luminescent component of the converted light in the red spectrum region led to an increase in plant growth and photosynthesis rate, which is a fundamentally new result. Possible reasons for the stimulation of growth and photosynthesis due to the redistribution of the light spectral composition were analyzed. The use of covering materials containing luminophores similar to LUM can be promising in agrobiotechnology not only for green and vegetable crops but also for other field and greenhouse crops and various fruit bushes and trees.
Loss of Multiple ABCB Auxin Transporters Recapitulates the Major twisted dwarf 1 Phenotypes in Arabidopsis thaliana
Authors: Jenness Mark K. , Tayengwa Reuben , Bate Gabrielle A. , Tapken Wiebke , Zhang Yuqin , Pang Changxu , Murphy Angus S.
Published: April 2022

FK506-BINDING PROTEIN 42/TWISTED DWARF 1 (FKBP42/TWD1) directly regulates cellular trafficking and activation of multiple ATP-BINDING CASSETTE (ABC) transporters from the ABCB and ABCC subfamilies. abcb1 abcb19 double mutants exhibit remarkable phenotypic overlap with twd1 including severe dwarfism, stamen elongation defects, and compact circinate leaves; however, twd1 mutants exhibit greater loss of polar auxin transport and additional helical twisting of roots, inflorescences, and siliques. As abcc1 abcc2 mutants do not exhibit any visible phenotypes and TWD1 does not interact with PIN or AUX1/LAX auxin transporters, loss of function of other ABCB auxin transporters is hypothesized to underly the remaining morphological phenotypes. Here, gene expression, mutant analyses, pharmacological inhibitor studies, auxin transport assays, and direct auxin quantitations were used to determine the relative contributions of loss of other reported ABCB auxin transporters (4, 6, 11, 14, 20, and 21) to twd1 phenotypes. From these analyses, the additional reduction in plant height and the twisted inflorescence, root, and silique phenotypes observed in twd1 compared to abcb1 abcb19 result from loss of ABCB6 and ABCB20 function. Additionally, abcb6 abcb20 root twisting exhibited the same sensitivity to the auxin transport inhibitor 1-napthalthalamic acid as twd1 suggesting they are the primary contributors to these auxin-dependent organ twisting phenotypes. The lack of obvious phenotypes in higher order abcb4 and abcb21 mutants suggests that the functional loss of these transporters does not contribute to twd1 root or shoot twisting. Analyses of ABCB11 and ABCB14 function revealed capacity for auxin transport; however, their activities are readily outcompeted by other substrates, suggesting alternate functions in planta, consistent with a spectrum of relative substrate affinities among ABCB transporters. Overall, the results presented here suggest that the ABCB1/19 and ABCB6/20 pairs represent the primary long-distance ABCB auxin transporters in Arabidopsis and account for all reported twd1 morphological phenotypes. Other ABCB transporters appear to participate in highly localized auxin streams or mobilize alternate transport substrates.
Genomic Analysis Based on Chromosome-Level Genome Assembly Reveals an Expansion of Terpene Biosynthesis of Azadirachta indica
Authors: Du Yuhui , Song Wei , Yin Zhiqiu , Wu Shengbo , Liu Jiaheng , Wang Ning , Jin Hua , Qiao Jianjun , Huo Yi-Xin
Published: April 2022

Azadirachta indica (neem), an evergreen tree of the Meliaceae family, is a source of the potent biopesticide azadirachtin. The lack of a chromosome-level assembly impedes an in-depth understanding of its genome architecture and the comparative genomic analysis of A. indica. Here, a high-quality genome assembly of A. indica was constructed using a combination of data from Illumina, PacBio, and Hi-C technology, which is the first chromosome-scale genome assembly of A. indica. Based on the length of our assembly, the genome size of A. indica is estimated to be 281 Mb anchored to 14 chromosomes (contig N50 = 6 Mb and scaffold N50 = 19 Mb). The genome assembly contained 115 Mb repetitive elements and 25,767 protein-coding genes. Evolutional analysis revealed that A. indica didn’t experience any whole-genome duplication (WGD) event after the core eudicot γ event, but some genes and genome segment might likely experienced recent duplications. The secondary metabolite clusters, TPS genes, and CYP genes were also identified. Comparative genomic analysis revealed that most of the A. indica-specific TPS genes and CYP genes were located on the terpene-related clusters on chromosome 13. It is suggested that chromosome 13 may play an important role in the specific terpene biosynthesis of A. indica. The gene duplication events may be responsible for the terpene biosynthesis expansion in A. indica. The genomic dataset and genomic analysis created for A. indica will shed light on terpene biosynthesis in A. indica and facilitate comparative genomic research of the family Meliaceae.
WHIRLIES Are Multifunctional DNA-Binding Proteins With Impact on Plant Development and Stress Resistance
Authors: Krupinska Karin , Desel Christine , Frank Susann , Hensel Götz
Published: April 2022

WHIRLIES are plant-specific proteins binding to DNA in plastids, mitochondria, and nucleus. They have been identified as significant components of nucleoids in the organelles where they regulate the structure of the nucleoids and diverse DNA-associated processes. WHIRLIES also fulfil roles in the nucleus by interacting with telomers and various transcription factors, among them members of the WRKY family. While most plants have two WHIRLY proteins, additional WHIRLY proteins evolved by gene duplication in some dicot families. All WHIRLY proteins share a conserved WHIRLY domain responsible for ssDNA binding. Structural analyses revealed that WHIRLY proteins form tetramers and higher-order complexes upon binding to DNA. An outstanding feature is the parallel localization of WHIRLY proteins in two or three cell compartments. Because they translocate from organelles to the nucleus, WHIRLY proteins are excellent candidates for transducing signals between organelles and nucleus to allow for coordinated activities of the different genomes. Developmental cues and environmental factors control the expression of WHIRLY genes. Mutants and plants with a reduced abundance of WHIRLY proteins gave insight into their multiple functionalities. In chloroplasts, a reduction of the WHIRLY level leads to changes in replication, transcription, RNA processing, and DNA repair. Furthermore, chloroplast development, ribosome formation, and photosynthesis are impaired in monocots. In mitochondria, a low level of WHIRLIES coincides with a reduced number of cristae and a low rate of respiration. The WHIRLY proteins are involved in the plants’ resistance toward abiotic and biotic stress. Plants with low levels of WHIRLIES show reduced responsiveness toward diverse environmental factors, such as light and drought. Consequently, because such plants are impaired in acclimation, they accumulate reactive oxygen species under stress conditions. In contrast, several plant species overexpressing WHIRLIES were shown to have a higher resistance toward stress and pathogen attacks. By their multiple interactions with organelle proteins and nuclear transcription factors maybe a comma can be inserted here? and their participation in organelle–nucleus communication, WHIRLY proteins are proposed to serve plant development and stress resistance by coordinating processes at different levels. It is proposed that the multifunctionality of WHIRLY proteins is linked to the plasticity of land plants that develop and function in a continuously changing environment.
Effect of Cognitive Reappraisal on Archery Performance of Elite Athletes: The Mediating Effects of Sport-Confidence and Attention
Authors: Wang Dongling , Hu Ti , Luo Rui , Shen Qiqi , Wang Yuan , Li Xiujuan , Qiao Jiang , Zhu Lina , Cui Lei , Yin Hengchan
Published: April 2022

Through empirical studies or laboratory tests, previous studies have shown that sport-confidence, attention, and emotion regulation are key factors in archery performance. The present study aims to further identify the effects and pathways of sport-confidence, attention, and cognitive reappraisal (a specific emotion regulation strategy) on real-world archery performance by constructing a hypothesized model to provide a basis for scientific training of athletes to improve sport performance. A survey design was utilized on a sample of 61 athletes (12 international-level athletes, 30 national-level athletes, and 19 first-class athletes) from the Chinese National Archery Team to test the model. The measurement and hypothesized models were tested using partial least squares structural equation modeling (PLS-SEM). The results indicate that the model fit well and explained 33.6% of the variance in archery performance. Sport-confidence (total effects = 0.574, p < 0.001) and attention (total effects = 0.344, p = 0.009) were important predictive indicators of archery performance, while the relationship between cognitive reappraisal and archery performance showed considerable complexity (direct effects = −0.268, p = 0.020; total effects = −0.007, p = 0.964). We conclude that the development of sport-confidence and attention of archery athletes should be strengthened, but athletes who use cognitive reappraisal in archery competition should be mindful of its potential appropriation of cognitive resources and should be directed to improve sport-confidence or develop a positive orientation to arouse excitement.
Arthroscopic Treatment of Popliteus Tendinitis Using the Accessory Portal
Authors: Chen B. , Liu H. K. , Wang H.
Published: April 2022

Introduction

This study aimed to evaluate the effect of arthroscopic treatment of popliteus tendinitis via an auxiliary extreme lateral approach and to investigate the pathogenesis and treatment of popliteus tendinitis.

Materials and Methods

From 2016 to 2020, arthroscopic popliteus tendon ablation was performed in 15 patients (15 knees) with popliteus tendinitis via an auxiliary extreme lateral approach. Clinical outcomes were assessed using the Lysholm knee scoring scale, the Tegner score, the International Knee Documentation Committee (IKDC) score and the visual analogue scale (VAS) pain score at the 24-month follow-up after surgery.

Results

A total of 15 patients (mean age, 51.1 ± 7.1 years) were included. They had a mean body mass index of 23.8 ± 2.1 kg/m2. The minimum follow-up period was 24 months. Comparing the postoperative state to the preoperative state, the mean postoperative Lysholm score, Tegner score, and IKDC score improved significantly from 70.0 ± 5.0, 3.0 ± 0.9, and 62.3 ± 5.5 to 89.3 ± 4.2, 4.6 ± 0.61, and 80.5 ± 4.4, respectively (p < 0.01). The preoperative VAS score for pain improved from 6.4 ± 0.5 to 0.9 ± 0.4 (p < 0.01). No patients were lost to follow-up.

Conclusions

Following arthroscopic-assisted treatment, all the patients with popliteus tendinitis achieved satisfactory clinical outcomes in terms of pain relief and improved function.

Level of Evidence

Level IV
Prognostic Significance of Pre- to Postoperative Dynamics of Sarcopenia for Patients with Renal Cell Carcinoma Undergoing Laparoscopic Nephrectomy
Authors: Chen Shuqiu , He Ting , Sun Si , Wu Jianping , Xu Bin , Mao Weipu , Chen Ming
Published: April 2022

Background

The aim of this study was to investigate the prognostic role of the dynamics of sarcopenia in the pre- to postoperative for patients with renal cell carcinoma (RCC) undergoing laparoscopic nephrectomy.

Methods

This study included 261 patients who underwent laparoscopic nephrectomy between 2014 and 2019. The skeletal muscle index (SMI) of the L3 lumbar region was used to assess sarcopenia. The overall population was divided into four groups according to the dynamics of sarcopenia from pre- to postoperative: group 1 (both pre- and postoperative sarcopenia), group 2 (preoperative non-sarcopenia to postoperative sarcopenia), group 3 (preoperative sarcopenia to postoperative non-sarcopenia), and group 4 (both pre- and postoperative non-sarcopenia). The endpoints of the study were overall survival (OS) and cancer-specific survival (CSS).

Results

Of the 261 patients who underwent laparoscopic nephrectomy, 103 (39.5%) had preoperative sarcopenia and 183 (70.1%) had postoperative sarcopenia. Patients with pre- or postoperative sarcopenia had poor survival outcomes. Sarcopenia dynamic was a better predictor of OS (AUC = 0.737) and CSS (AUC = 0.696) in patients with RCC than pre- and postoperative sarcopenia, and patients in group 4 of sarcopenia dynamic had the best OS and CSS. In addition, sarcopenia dynamics was an independent risk factor for OS and CSS, with a 94.5% reduction in OS risk (HR = 0.055, 95% CI 0.007–0.407, p = 0.003) and a 91.9% reduction in CSS risk (HR = 0.081, 95% CI 0.011–0.616, p = 0.015) in the group 4 compared with the group 1.

Conclusion

Our study is the first to assess the prognostic value of pre- and postoperative sarcopenia dynamics in patients with RCC.
Case Report: Intraoperative Fascial Traction in Robotic Abdominal Wall Surgery; An Early Experience
Authors: Bloemendaal A. L. A.
Published: April 2022

Intraoperative fascial traction (IFT) may obviate the use of a posterior component separation/transversus abdominis release (TAR). Robotic abdominal wall surgery leads to a reduction of morbidity in TAR compared to open surgery. The combination of minimally invasive (robotic) abdominal wall surgery with IFT may lead to a further reduction of surgical morbidity.
A Battery-Powered Fluid Manipulation System Actuated by Mechanical Vibrations
Authors: Akkoyun Fatih , Özçelik Adem
Published: April 2022

Miniaturized fluid manipulation systems are an important component of lab-on-a-chip platforms implemented in resourced-limited environments and point-of-care applications. This work aims to design, fabricate, and test a low-cost and battery-operated microfluidic diffuser/nozzle type pump to enable an alternative fluid manipulation solution for field applications. For this, CNC laser cutting and 3D printing are used to fabricate the fluidic unit and casing of the driving module of the system, respectively. This system only required 3.5-V input power and can generate flow rates up to 58 µL/min for water. In addition, this portable pump can manipulate higher viscosity fluids with kinematic viscosities up to 24 mPa·s resembling biological fluids such as sputum and saliva. The demonstrated system is a low-cost, battery-powered, and highly versatile fluid pump that can be adopted in various lab-on-a-chip applications for field deployment and remote applications.
Mathematical Models of Leaf Area Index and Yield for Grapevines Grown in the Turpan Area, Xinjiang, China
Authors: Su Lijun , Tao Wanghai , Sun Yan , Shan Yuyang , Wang Quanjiu
Published: April 2022

The Leaf Area Index (LAI) strongly influences crop biomass production and yields. The variation characteristic of LAI and the development of crop growth models can provide a theoretical basis for predicting crops’ water consumption, fruit quality and yields. This paper analyzes the relationship between measurements of aboveground grape biomass and trends in LAI and dry biomass production in grapes grown in the Turpan area. The LAI changes in grapes were estimated using the modified logistic model, the modified Gaussian model, the log-normal model, the cubic polynomial model, and the Gaussian model. Universal models of LAI were established in which the applied irrigation quota was applied to calculate the maximum LAI. The relationship between the irrigation quota and biomass production, yields, and the harvest index was investigated. The developed models could accurately predict the LAI of grapevines grown in an extremely arid area. However, the Gaussian and cubic polynomial models produced less accurate results than the other models tested. The Michaelis–Menten model analyzed the relationship between biomass and LAI, providing a numerical method for predicting dynamic changes in grapevine LAI. Moreover, the crop biomass increased linearly with the irrigation quota for quotas between 6375 and 13,200 m3/hm. This made it possible to describe the grape yield and harvest index with a quadratic polynomial function, which increases during the early stages of the growing season and then decreases. The analyses of the relationship between yield and harvest index provide important theoretical insights that can be used to improve water use efficiency in grape cultivation and to identify optimal irrigation quotas.
Integrating Genome-Wide Association Study with Transcriptomic Analysis to Predict Candidate Genes Controlling Storage Root Flesh Color in Sweet Potato
Authors: Liu Yi , Pan Rui , Zhang Wenying , Lei Jian , Wang Lianjun , Chai Shasha , Jin Xiaojie , Jiao Chunhai , Yang Xinsun
Published: April 2022

Sweet potato is a hexaploid heterozygote with a complex genetic background, self-pollination infertility, and cross incompatibility, which makes genetic linkage analysis quite difficult. Genome-wide association studies (GWAS) provide a new strategy for gene mapping and cloning in sweet potato. Storage root flesh color (SRFC) is an important sensory evaluation, which correlates with storage root flesh composition, such as starch, anthocyanin, and carotenoid. We performed GWAS using SRFC data of 300 accessions and 567,828 single nucleotide polymorphism (SNP) markers. Furthermore, we analyzed transcriptome data of different SRFC varieties, and conducted real-time quantitative PCR (qRT-PCR) to measure the expression level of the candidate gene in purple and non-purple fleshed sweet potato genotypes. The results showed that five unique SNPs were significantly (−log10P > 7) associated with SRFC. Based on these trait-associated SNPs, four candidate genes, g55964 (IbF3′H), g17506 (IbBAG2-like), g25206 (IbUGT-73D1-like), and g58377 (IbVQ25-isoform X2) were identified. Expression profiles derived from transcriptome data and qRT-PCR analyses showed that the expression of g55964 in purple-fleshed sweet potato was significantly (p < 0.01) higher than that of non-purple fleshed sweet potato. By combining the GWAS, transcriptomic analysis and qRT-PCR, we inferred that g55964 is the key gene related to purple formation of storage root in sweet potato. Our results lay the foundation for accelerating sweet potato genetic improvement of anthocyanin through marker-assisted selection.
Housing and Environmental Enrichment of the Domestic Ferret: A Multi-Sector Survey
Authors: Dancer Alice , Díez-León María , Bizley Jennifer , Burn Charlotte
Published: April 2022

Ferrets (Mustela putorius furo) are kept and used in multiple sectors of society, but little is known about how they are housed and what environmental enrichment (EE) they may benefit from. We aimed to help guide caretakers about what housing and EE can be provided for ferrets. Through an online questionnaire of ferret caretakers, including pet, laboratory, zoological collection, rescue and working animal sectors internationally, we described ferret housing, opportunities for exploration, EE provision and caretaker opinions on ferrets’ preferred EE types, and problematic EE. In total, 754 valid responses from 17 countries were analysed, with most (82.4%) coming from pet owners. Most ferrets were housed socially, with housing varying across sectors from single-level cages to free-range housing in a room or outdoor enclosure; pet owners mostly used multi-level cages. The most commonly reported EE included hammocks, tunnels and tactile interaction with caretakers. Respondents reported that ferrets particularly enjoyed digging substrates, tunnels, human interaction and exploration. The most frequently reported problems were that ingestion of unsuitable chew toys and rubber items could cause internal blockages, narrow tunnels could trap ferrets, and certain fabrics that could catch claws. This suggests a need for increased awareness of the risks of these EE types and for more commercially available safety-tested ferret EE. Scent trails were relatively rarely provided but were reported to be enjoyed and harmless, so we recommend that these should be provided more commonly. Our results suggest that there is scope to improve ferret housing and EE provision to benefit ferret welfare across all sectors.
Sentience Matters: Analysing the Regulation of Calf-Roping in Australian Rodeos
Authors: Stonebridge Morgan , Evans Di , Kotzmann Jane
Published: April 2022

Animal sentience is recognised either implicitly or explicitly in legislation in all Australian states and territories. In these jurisdictions, animal welfare legislation prohibits acts of cruelty towards animals because animals have the capacity to experience pain or suffering. This acknowledgement is supported by scientific research that demonstrates animal sentience, as well as public opinion. Despite these legal prohibitions, calf-roping, a common event at rodeos, is permitted in the majority of Australian jurisdictions. In recent times, calf-roping has generated significant public concern due to the potential for injury, pain or distress for the calves involved. This concern is evidently shared in some overseas jurisdictions, such as New Zealand, where animal advocacy organisations have filed a legal challenge asserting that rodeo events violate New Zealand’s animal welfare legislation due to the pain and distress inflicted on the animals. This commentary discusses these welfare concerns, the legislative inconsistencies between Australian jurisdictions and the problematic legal status of calf-roping in Australia.
Fecal Glucocorticoid Metabolites as a Noninvasive Indicator of Stress in the Tsushima Leopard Cats (Prionailurus bengalensis euptilurus): Application to Health Care
Authors: Kusuda Satoshi , Funahashi Takashi , Adachi Itsuki , Yamamoto Hanae , Nagao Eiji , Matsui Kirito , Akiba Yuki
Published: April 2022

This study investigates whether the measurement of glucocorticoid metabolites (GCMs) in feces is a useful method for the noninvasive evaluation of stress in the endangered Tsushima leopard cats (Prionailurus bengalensis euptilurus). Feces were collected from six seemingly healthy and five diseased (renal dysfunction, adrenal tumor, hernia, feline immunodeficiency virus (FIV), feline leukemia virus (FeLV)) Tsushima leopard cats in captivity. Fecal GCMs were measured by enzyme immunoassay (EIA) for cortisol. Individuals that experienced a physical examination under anesthesia showed increased fecal GCMs 1–2 days after the event. An individual diagnosed with disk herniation showed decreased fecal GCMs after medical administration. The mean fecal GCM concentrations for six healthy animals and five diseased animals were 0.66 ± 0.08 and 2.65 ± 0.76 μg/g, respectively, which was significantly different. Cortisol and corticosterone were not clearly detected in the feces examined by the use of the HPLC-EIA analysis. GCMs may be excreted in the feces; however, the exact identification of these substances is not achieved. The results suggest that the measurement of fecal GCMs is useful for the husbandry and health management of this species.
Research on an Improved SOM Model for Damage Identification of Concrete Structures
Authors: Liu Jinxin , Li Kexin
Published: April 2022

In order to solve the problem of intelligent detection of damage of modern concrete structures under complex constraints, an improved self-organizing mapping (SOM) neural network model algorithm was proposed to construct an accurate identification model of concrete structure damage. Based on the structure and algorithm of the SOM network model, the whole process of the core construction of the concrete structure damage identification network model is summarized. Combined with the damage texture characteristics of concrete structures, through the self-developed 3D laser scanning system, an improved method based on a small number of samples to effectively improve the effectiveness of network input samples is proposed. Based on the principle of network topology map analysis and its image characteristics, a SOM model improvement method that can effectively improve the accuracy of the network identification model is studied. In addition, based on the reactive powder concrete bending fatigue loading test, the feasibility and accuracy of the improved method are verified. The results show that the improved SOM concrete structure damage identification model can effectively identify unknown neuron categories in a limited sample space, and the identification accuracy of the SOM network model is improved by 4.69%. The proposed improved SOM model method fully combines the network topology and its unique image features and can accurately identify structural damage. This research contributes to the realization of high-precision intelligent health monitoring of damage to modern concrete structures. In addition, it is of great significance for the timely detection, identification and localization of early damage to structures.
“Wings of Freedom”: Petr Miturich and Aero-Constructivism
Authors: Bowlt John E.
Published: April 2022

The article focuses on the aerodynamic experiments of Petr Vasil’evich Miturich (1887–1956), in particular his so-called letun, a project comparable to Vladimir Tatlin’s Letatlin, but less familiar. Miturich became interested in flight during the First World War, elaborating his first flying apparatus in 1918 before constructing a prototype and undertaking a test flight on 27 December 1921—which might be described as an example of Russian Aero-Constructivism (by analogy with Italian Aeropittura). Miturich’s basic deduction was that modern man must travel not by horse and cart, but with the aid of a new, ecological apparatus—the undulator—a mechanism which, thanks to its undulatory movements, would move like a fish or snake. The article delineates the general context of Miturich’s experiments, for example, his acquaintance with the ideas of Tatlin and Velemir Khlebnikov (in 1924 Miturich married the artist, Vera Khlebnikova, Velemir’s sister) as well as the inventions of Igor’ Sikorsky, Fridrikh Tsander, Konstantin Tsiolkovsky and other scientists who contributed to the “First Universal Exhibition of Projects and Models of Interplanetary Apparatuses, Devices and Historical Materials” held in Moscow in 1927.
Chemical Composition Characteristics and Source Contributions of Precipitation in Typical Cities on the North Slope of Tianshan Mountain in Xinjiang during 2010–2019
Authors: Zhong Yuting , Li Xia , Fan Ziang , Ayitken Maulen , Li Shuting , Liu Xinchun
Published: April 2022

This work presents the results of a ten-year investigation (2010–2019) on the characteristics and sources of precipitation pollution in typical cities locating in the economic belt on the North Slope of Tianshan Mountain, Xinjiang. The water-soluble ions’ characteristics (Na+, NH4+, K+, Mg2+, Ca2+, F−, Cl−, SO42−, NO3−), neutralizing capacity, wet deposition and sources of precipitation in Urumqi, Karamay and Yining during 2010–2019 were compared and analyzed. The study showed that from 2010 to 2019, the pH value of precipitation in Urumqi, Karamay and Yining varied from 4.18 to 10.55 with a volume-weighted mean (VWM) pH of 6.33, and the pH value showed an upward trend overall. The VWM electrical conductivity (EC) of the precipitation was the highest in Urumqi and the lowest in Yining, indicating that Urumqi was the most polluted and Yining was relatively clean. The most important cation in the precipitation of the three cities was Ca2+, and the most important anion was SO42−. The ratio of SO42−/NO3− indicated that the air pollution in Urumqi and Yining belonged to the typical coal-smoke air pollution, while there was compound pollution in Karamay. As can be seen from the neutralization factor, Ca2+ had the strongest neutralization ability, followed by Na+ and NH4+. Nitrate and sulfate in the atmosphere of Urumqi, Karamay and Yining are likely to exist in the form of NH4NO3, CaSO4 and (NH4)2SO4•CaSO4•2H2O. Wet deposition flux analysis of S and N showed that S pollution of the precipitation in Urumqi showed a decreasing trend, while N pollution showed an increasing trend. S pollution of the precipitation in Karamay gradually decreased, while N pollution of the precipitation in Yining became more and more serious. By analyzing origins of major ions in precipitation, it is concluded that human activities (industry, agriculture, heating, and transportation) are the main sources of ions in precipitation, and natural sources (soil dust) also play an important role.
Airmass Analysis of Size-Resolved Black Carbon Particles Observed in the Arctic Based on Cluster Analysis
Authors: Cremer Roxana S. , Tunved Peter , Ström Johan
Published: April 2022

Here we apply new analysis methods and approaches to existing long-term measurement series that provide additional insights into the atmospheric processes that control black carbon (BC) in the Arctic. Based on clustering size distribution data from Zeppelin Observatory for the years 2002–2010, observations classified as ‘Polluted’ were further investigated based on BC properties. The data were split into two subgroups, and while the microphysical and chemical fingerprints of the two subgroups are very similar, they show larger differences in BC concentration and correlation with the particle size distribution. Therefore, a source–receptor analysis was performed with HYSPLIT 10-days backward trajectories for both subsets. We demonstrate that within this ‘Polluted’ category, the airmasses that contributed to the largest BC signal at the Zeppelin station are not necessarily associated with traditional transport pathways from Eurasia. Instead, the strongest signal is from a region east of the Ural Mountains across the continent to the Kamchatka Peninsula.
Comparison of Relative and Absolute Heatwaves in Eastern China: Observations, Simulations and Future Projections
Authors: Xu Haoran , Zhang Guwei
Published: April 2022

Heatwaves can produce catastrophic effects on public health and natural systems, especially under global warming. There are two methods to measure heatwaves, computed by relative and absolute thresholds, namely relative and absolute heatwaves (RHWs and AHWs). Generally, AHWs mostly occur in hot areas because of fixed thresholds, while RHWs represent anomalous events for the local climate, making them possible everywhere in the warm season. Based on observations and CMIP6 outputs, this study compared AHWs and RHWs in Eastern China (EC) with five sub-regions [Northeast China (NEC), North China (NC), Lower Yangtze River (LYR), Middle Yangtze River (MYR) and South China (SC)]. Similarities among RHWs and AHWs were found in present-day trends (1995–2014) and spatial distributions. The heatwave intensity/days for RHWs and AHWs both displayed highest future increases in northern/southern EC, and the increases for 2081–2100 would be 1.5 times as high as 2041–2060. All these similarities illustrate that applying either relative or absolute thresholds in EC, historical temporal variations, changing future spatial patterns, and increasing ratio from 2081–2100 to 2041–2060, would show reliable results. As far as differences are concerned, RHWs were observed across the entire EC, while AHWs did not show up in parts of NC and NEC. Considering model performance, RHWs would perform better than AHWs in most areas of EC. The annual heatwave intensity/days were higher for RHWs than for AHWs during present-day and future periods, which might overestimate heat-related risks. Overall, this study recommended RHWs for heatwave analyses, particularly for future projections, but for risk assessment, the choice of thresholds is crucial. The results reinforced the necessity to further improve model performance to address various needs.
Traveling 10-Day Waves at Mid-Latitudes in the Troposphere and Lower Stratosphere Revealed by Radiosonde Observations and MERRA-2 Data in 2020
Authors: Yang Lingyun , Huang Chunming , Zhang Shaodong , Huang Kaiming , Gong Yun , Ma Zheng , Zhang Jian , Fan Yi
Published: April 2022

Although the characteristics of the traveling 10-day waves (10DWs) above the middle stratosphere have been well explored, little research has been performed on the counterpart in the troposphere and lower stratosphere (TLS). In the present study, we use radiosonde observations and MERRA-2 data from 2020 to characterize traveling 10DWs in mid-latitudes in the TLS. Single-site observations in both hemispheres show that strong 10DW activities are always accompanied by strong eastward jets (10–13 km). MERRA-2 data indicate that in the troposphere, the eastward-propagating modes with larger wavenumbers, i.e., E3, E4, E5 and E6 are dominant, while in the lower stratosphere, the eastward- and westward-propagating modes with small zonal wavenumbers, e.g., 1 and 2, are dominant. Further research on E3, E4, E5 and E6 modes in the troposphere of both hemispheres shows that all the wave activities are positively correlated to the background zonal wind. The refractive index squared reveals that a strong eastward jet is suitable for these four modes to propagate. However, just above the jet, the eastward wind decreases with altitude, and a thick evanescence region emerges above 15 km. E3, E4, E5 and E6 10DWs cannot propagate upward across the tropopause; as such, this can explain why these four modes are weak or even indiscernible in the stratosphere and above. In the troposphere, E5 10DW at 32° S was the most dominant mode in 2020. A case study of the anomalously strong E5 10DW activity on 12 May 2020 indicates that the wave amplification resulted from the upward and equatorward transmission of wave energy flows. Moreover, the tropopause and equatorial region can prevent the propagations of wave energy flows of E5 10DW. The upward-propagating wave energy is deposited near the tropopause, resulting in significant short-term variability in the tropopause height.
Solving Integral Equations Using Weakly Compatible Mappings via D*-Metric Spaces
Authors: Butush Roqia , Mustafa Zead , Jaradat M. M. M.
Published: April 2022

We introduce a new pair of mappings (S,T) on D*-metric spaces called DS*-W.C. and DRS*-W.C. Many examples are presented to show the difference between these mappings and other types of mappings in the literature. Moreover, we obtain several common fixed point results by using these types of mappings and the (E.A) property. We then employ the fixed point results to establish the existence and uniqueness of a solution for a class of nonlinear integral equations.
Modification of Zirconia Implant Surfaces by Nd:YAG Laser Grooves: Does It Change Cell Behavior?
Authors: da Cruz Mariana Brito , Marques Joana Faria , Marques Ana Filipa Silva , Madeira Sara , Carvalho Óscar , Silva Filipe , Caramês João , da Mata António Duarte Sola Pereira
Published: April 2022

The aim of this study was to evaluate gingival fibroblasts and human osteoblasts’ response to textured Nd:YAG laser microgrooves, with different dimensions, on zirconia implant surfaces. A total of 60 zirconia disks (8 mm in diameter and 2 mm in thickness) were produced and divided between four study groups (N = 15): three laser-textured (widths between 125.07 ± 5.29 μm and 45.36 ± 2.37 μm and depth values from 50.54 ± 2.48 μm to 23.01 ± 3.79 μm) and a control group without laser treatment. Human osteoblasts and gingival fibroblasts were cultured on these surfaces for 14 days. FEG-SEM (Field Emission Gun–Scanning Electron Microscope) images showed cellular adhesion at 24 h, with comparable morphology in all samples for both cell types. A similar cell spreading within the grooves and in the space between them was observed. Cell viability increased over time in all study groups; however, no differences were found between them. Additionally, proliferation, ALP (Alkaline phosphatase) activity, collagen type I, osteopontin and interleukin levels were not significantly different between any of the study groups for any of the cell types. Analysis of variance to compare parameters effect did not reveal statistically significant differences when comparing all groups in the different tests performed. The results obtained revealed similar cell behavior based on cell viability and differentiation on different microtopographic laser grooves, compared to a microtopography only established by sandblasting and acid-etching protocol, the reference surface treatment on zirconia dental implants.
Chaperone Copolymer Assisted G-Quadruplex-Based Signal Amplification Assay for Highly Sensitive Detection of VEGF
Authors: Han Jialun , Fang Chenxin , Ouyang Ping , Qing Yang , Yang Yuxing , Li Haiyu , Wang Zhencui , Du Jie
Published: April 2022

Vascular endothelial growth factor (VEGF) is a critical biomarker in the angiogenesis of several cancers. Nowadays, novel approaches to rapid, sensitive, and reliable VEGF detection are urgently required for early cancer diagnosis. Cationic comb-type copolymer, poly(L-lysine)-graft-dextran (PLL-g-Dex) accelerates DNA hybridization and chain exchange reaction while stabilizing the DNA assembly structure. In this work, we examined the chaperone activity of PLL-g-Dex to assist G-quadruplex-based fluorescent DNA biosensors for sensitive detection of VEGF. This convenient and effective strategy is based on chitosan hydrogel, c-myc, Thioflavin T (ThT), VEGF aptamer, and its partially complementary strand. The results show that chaperone copolymer PLL-g-Dex significantly promotes the accumulation of G-quadruplex and assembles into G-wires, allowing an effective signal amplification. Using this method, the detection limit of VEGF was as low as 23 pM, better than many previous works on aptamer-based VEGF detection. This chaperone copolymer-assisted signal amplification strategy has potential applications in the highly sensitive detection of target proteins, even including viruses.
Isolated Cervical Ruptured Radiculomedullary Artery Aneurysm Predominantly Presenting as Supratentorial Subarachnoid Hemorrhage: Case Report and Review of the Literature
Authors: Chen Fuxiang , Lu Wen , Lian Baoqiang , Kang Dezhi , Dai Linsun
Published: April 2022

A spinal artery aneurysm originating from the branch of the extracranial vertebral artery is uncommon. Most of them were finally diagnosed due to the evidence of infratentorial or spinal subarachnoid hemorrhage (SAH). Herein, we report an extremely rare case of a ruptured spinal artery aneurysm which predominantly presented with supratentorial SAH. A 68-year-old woman was initially revealed cranial computed tomographic angiographically negative SAH with a Hunt-Hess grade of 3, while the digital subtraction angiography confirmed an isolated radiculomedullary aneurysm arising from the medial ascending branch of V2 segment at C2 level. The patient underwent surgery in a hybrid operating room. She was originally attempted with coil embolization, but successful clipping of the aneurysm was achieved through unilateral laminectomy at last. Regrettably, the current case suffered a poor clinical outcome due to the complications caused by progressive cerebral vasospasm. In summary, angiogram is of great value for this rare kind of aneurysmal definitive diagnosis. A hybrid operating room may be a feasible choice for the ruptured spinal artery aneurysm.
Site and Mechanism of Recurrent Pontine Infarction: A Hospital-Based Follow-Up Study
Authors: Wu Li , Li Youfu , Ye Zeming , Liu Dezhi , Dai Zheng , Zhu Juehua , Chen Hongbing , Li Chenghao , Lie Chaowei , Jiang Yongjun
Published: April 2022

Although pontine infarction is the most common subtype of posterior circulation stroke, there has been little research focusing on recurrent pontine infarction. Our study aimed to investigate the factors associated with site and mechanism of recurrent pontine infarction. Patients with acute isolated pontine infarction were enrolled and followed up for one year. Lesion topography was determined by diffusion-weighted imaging. Mechanisms were determined based on lesion topography and other vascular, cardiologic and laboratory results. A total of 562 patients with pontine infarction were included, with 67 patients experiencing recurrence during the follow-up period. Forty-one recurrences occurred at the same site as index pontine infarction (41/67, 61.2%). Results indicated that the mechanism of index pontine infarction was significantly associated with the recurrent sites (p = 0.041, OR 2.938, 95% CI 1.044–8.268), and also with the mechanisms of recurrence (p = 0.004, OR 6.056, 95% CI 1.774–20.679). Branch atheromatous disease-induced index pontine infarction was likely to recur at the same site and with the same mechanism. Moreover, if recurrence occurred at the same site, the mechanism was probably the same as that of the index stroke (p = 0.000). Our study may help physicians treat patients with pontine infarction by predicting the site and mechanism of recurrence.
Role of Respiratory Epithelial Cells in Allergic Diseases
Authors: Jakwerth Constanze A. , Ordovas-Montanes Jose , Blank Simon , Schmidt-Weber Carsten B. , Zissler Ulrich M.
Published: April 2022

The airway epithelium provides the first line of defense to the surrounding environment. However, dysfunctions of this physical barrier are frequently observed in allergic diseases, which are tightly connected with pro- or anti-inflammatory processes. When the epithelial cells are confronted with allergens or pathogens, specific response mechanisms are set in motion, which in homeostasis, lead to the elimination of the invaders and leave permanent traces on the respiratory epithelium. However, allergens can also cause damage in the sensitized organism, which can be ascribed to the excessive immune reactions. The tight interaction of epithelial cells of the upper and lower airways with local and systemic immune cells can leave an imprint that may mirror the pathophysiology. The interaction with effector T cells, along with the macrophages, play an important role in this response, as reflected in the gene expression profiles (transcriptomes) of the epithelial cells, as well as in the secretory pattern (secretomes). Further, the storage of information from past exposures as memories within discrete cell types may allow a tissue to inform and fundamentally alter its future responses. Recently, several lines of evidence have highlighted the contributions from myeloid cells, lymphoid cells, stromal cells, mast cells, and epithelial cells to the emerging concepts of inflammatory memory and trained immunity.
Cell Aggregate Assembly through Microengineering for Functional Tissue Emergence
Authors: Eke Gozde , Vaysse Laurence , Yao Xi , Escudero Mélanie , Carrière Audrey , Trevisiol Emmanuelle , Vieu Christophe , Dani Christian , Casteilla Louis , Malaquin Laurent
Published: April 2022

Compared to cell suspensions or monolayers, 3D cell aggregates provide cellular interactions organized in space and heterogeneity that better resume the real organization of native tissues. They represent powerful tools to narrow down the gap between in vitro and in vivo models, thanks to their self-evolving capabilities. Recent strategies have demonstrated their potential as building blocks to generate microtissues. Developing specific methodologies capable of organizing these cell aggregates into 3D architectures and environments has become essential to convert them into functional microtissues adapted for regenerative medicine or pharmaceutical screening purposes. Although the techniques for producing individual cell aggregates have been on the market for over a decade, the methodology for engineering functional tissues starting from them is still a young and quickly evolving field of research. In this review, we first present a panorama of emerging cell aggregates microfabrication and assembly technologies. We further discuss the perspectives opened in the establishment of functional tissues with a specific focus on controlled architecture and heterogeneity to favor cell differentiation and proliferation.