bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel CDK4/6 and PARP Dual Inhibitor ZC-22 Effectively Suppresses Tumor Growth and Improves the Response to Cisplatin Treatment in Breast and Ovarian Cancer
Authors: Tian Chenchen , Wei Yufan , Li Jianjun , Huang Zhi , Wang Qiong , Lin Yingxue , Lv Xingping , Chen Yanan , Fan Yan , Sun Peiqing , Xiang Rong , Chang Antao , Yang Shuang
Published: March 2022

In recent years, three PARP inhibitors and three CDK4/6 inhibitors have been approved by the FDA for the treatment of recurrent ovarian cancer and advanced ER-positive breast cancer, respectively. However, the clinical benefits of the PARPi or CDK4/6i monotherapy are not as satisfied as expected and benefit only a fraction of patients. Current studies have shown therapeutic synergy for combinations of PARPi and CDK4/6i in breast and ovarian cancers with homologous recombination (HR) proficiency, which represents a new synthetic lethal strategy for treatment of these cancers regardless HR status. Thus, any compounds or strategies that can combine PARP and CDK4/6 inhibition will likely have great potential in improving clinic outcomes and in benefiting more patients. In this study, we developed a novel compound, ZC-22, that effectively inhibited both PARP and CDK4/6. This dual-targeting compound significantly inhibited breast and ovarian cancer cells by inducing cell cycle arrest and severe DNA damage both in vitro and in vivo. Interestingly, the efficacy of ZC-22 is even higher than the combination of PARPi Olaparib and CDK4/6i Abemaciclib in most breast and ovarian cancer cells, suggesting that it may be an effective alternative for the PARPi and CDK4/6i combination therapy. Moreover, ZC-22 sensitized breast and ovarian cancer cells to cisplatin treatment, a widely used chemotherapeutic agent. Altogether, our study has demonstrated the potency of a novel CDK4/6 and PARP dual inhibitor, which can potentially be developed into a monotherapy or combinatorial therapy with cisplatin for breast and ovarian cancer patients with HR proficiency.
Seasonal Flight Pattern of the Kissing Bugs Triatoma rubida and T. protracta (Hemiptera: Reduviidae: Triatominae) in Southern Arizona, United States
Authors: Schmidt Justin O. , Miller Mary L. , Klotz Stephen A.
Published: March 2022

The two most common kissing bugs, Triatoma rubida and T. protracta, in the Sonoran Desert around Tucson, Arizona are hematophagous vectors of Chagas disease and can induce potentially life-threatening allergic reactions. They were surveyed during their summer dispersal flight period to determine which environmental factors are correlated with flight activity. The two most important factors governing flights of T.rubida were temperatures in the range of 26–35 °C and wind speeds below 14 km/h (9 miles/h). Flights were reduced below or above those temperatures, or when wind speeds exceeding 14km/h. Relative humidity and presence or absence of moonshine appeared unimportant. During their dispersal flight periods of May through July and, especially, between the peak of the flight season, 20 June to 5 July, biologists seeking to collect bugs and homeowners wishing to exclude these biting bugs from entering their homes should be most attentive during evenings of average temperature and low wind speed.
Feasibility of 4D-Spatio Temporal Image Correlation (STIC) in the Comprehensive Assessment of the Fetal Heart Using FetalHQ®
Authors: Nogué Laura , Gómez Olga , Izquierdo Nora , Mula Cristina , Masoller Narcís , Martínez Josep M. , Gratacós Eduard , Devore Greggory , Crispi Fàtima , Bennasar Mar
Published: March 2022

Fetal Heart Quantification (FetalHQ®) is a novel speckle tracking software that permits the study of global and regional ventricular shape and function from a 2D four-chamber-view loop. The 4D-Spatio Temporal Image Correlation (STIC) modality enables the offline analysis of optimized and perfectly aligned cardiac planes. We aimed to evaluate the feasibility and reproducibility of 4D-STIC speckle tracking echocardiography (STE) using FetalHQ® and to compare it to 2D STE. We conducted a prospective study including 31 low-risk singleton pregnancies between 20 and 40 weeks of gestation. Four-chamber view volumes and 2D clips were acquired with an apex pointing at 45° and with a frame rate higher than 60 Hz. Morphometric and functional echocardiography was performed by FetalHQ®. Intra- and interobserver reproducibility were evaluated by the intraclass correlation coefficient (ICC). Our results showed excellent reproducibility (ICC > 0.900) for morphometric evaluation (biventricular area, longitudinal and transverse diameters). Reproducibility was also good (ICC > 0.800) for functional evaluation (biventricular strain, Fractional Area Change, left ventricle volumes, ejection fraction and cardiac output). On the contrary, the study of the sphericity index and shortening fraction of the different ventricular segments showed lower reproducibility (ICC < 0.800). To conclude, 4D-STIC is feasible, reproducible and comparable to 2D echocardiography for the assessment of cardiac morphometry and function.
Prognosis of Coronary Artery Bypass Grafting in Preschool-Aged Patients with Myocardial Ischemia Due to Giant Aneurysm of Kawasaki Disease
Authors: Watanabe Makoto , Fukazawa Ryuji , Kamisago Mitsuhiro , Ohkubo Takashi , Abe Masanori , Ochi Masami , Nitta Takashi , Ishii Yohsuke , Ogawa Shunichi , Itoh Yasuhiko
Published: March 2022

Coronary artery bypass grafting (CABG) for severe cardiac sequelae of Kawasaki disease (KD) complicated by myocardial ischemia is feasible even in childhood. However, no report has summarized the prognosis of CABG in preschool-aged children. Therefore, we evaluated the outcomes of seven preschool-aged children who underwent CABG for the cardiac sequelae of KD in our hospital. The median age at KD onset and CABG was 36 and 59 months, respectively. The median period from KD onset to CABG was 12 months. The median post-operative observation period was 108 months. CABG between the left internal thoracic artery and left anterior descending artery was performed in all patients. In all patients, postoperative cardiac catheter examination revealed good graft patency and no anastomotic stenosis. Further, pre-operative abnormality of coronary flow reserve returned to normal after CABG. Currently, only one patient is taking warfarin. Regarding school–life management, no patient has exercise limitations, except for one patient who had acute myocardial infarction before CABG. Further, the risk of graft stenosis or occlusion was evaluated in the included patients. However, no accidents have been reported to date, and myocardial ischemia and school–life management have improved. Thus, CABG is an effective treatment in preschool-aged children.
Small Cell Lung Cancer Transformation following Treatment in EGFR-Mutated Non-Small Cell Lung Cancer
Authors: Mambetsariev Isa , Arvanitis Leonidas , Fricke Jeremy , Pharaon Rebecca , Baroz Angel R. , Afkhami Michelle , Koczywas Marianna , Massarelli Erminia , Salgia Ravi
Published: March 2022

EGFR-mutated lung adenocarcinoma patients who received tyrosine kinase inhibitors (TKIs) may initially respond to therapy, but over time, resistance eventually occurs. In a small population (5–10%), these patients can have a histological transformation to SCLC. Nine patients with EGFR-mutated lung adenocarcinoma who transformed to SCLC were evaluated at City of Hope. Patient clinical and pathology data, including multiple next-generation sequencing (NGS) results, clinical therapies, histology, and outcomes, were collected across multiple time points. Descriptive statistics were utilized to visualize and interpret the clinical therapeutic timeline and molecular transformation profiles for these patients. All patients received at least one line of EGFR TKI therapies prior to small cell lung cancer transformation, including erlotinib, afatinib, and osimertinib. Two patients also received chemotherapy prior to transformation (one with immunotherapy). The median months to small cell lung cancer transformation was 16 months, ranging from 4–49 months. The median overall survival (OS) was 29 months from diagnosis, with the minimum of 16 months and maximum of 62 months. The majority of patients had EGFR exon 19 deletion (n = 7, 77.8%), and no patients had a change of original oncogenic EGFR mutation over the different time points. Though a TP53 mutation was detected in eight patients (88.9%) either at the first biopsy or the subsequent biopsies, an RB1 alteration was only detected in one patient at presentation, and three patients upon subsequent biopsies (n = 4, 44.4%). Each patient had a unique molecular profile in the subsequent molecular testing post-transformation, but BRAF alterations occurred frequently, including BRAF rearrangement (n = 1), fusion (n = 1), and amplification (n = 1). Our results showed that EGFR-mutated lung adenocarcinoma to SCLC transformation patients have a unique histological, molecular, and clinical profile over multiple time points, with further heterogeneity that is not currently reported in the literature, and we suggest more work is required to better understand the molecular heterogeneity and clinical outcomes over time for this EGFR TKI resistance subtype.
The GenoDiabMar Registry: A Collaborative Research Platform of Type 2 Diabetes Patients
Authors: Sierra Adriana , Otero Sol , Rodríguez Eva , Faura Anna , Vera María , Riera Marta , Palau Vanesa , Durán Xavier , Costa-Garrido Anna , Sans Laia , Márquez Eva , Poposki Vladimir , Franch-Nadal Josep , Mundet Xavier , Oliveras Anna , Crespo Marta , Pascual Julio , Barrios Clara
Published: March 2022

The GenoDiabMar registry is a prospective study that aims to provide data on demographic, biochemical, and clinical changes in type 2 diabetic (T2D) patients attending real medical outpatient consultations. This registry is also used to find new biomarkers related to the micro- and macrovascular complications of T2D, with a particular focus on diabetic nephropathy. With this purpose, longitudinal serum and urine samples, DNA banking, and data on 227 metabolomics profiles, 77 immunoglobulin G glycomics traits, and other emerging biomarkers were recorded in this cohort. In this study, we show a detailed longitudinal description of the clinical and analytical parameters of this registry, with a special focus on the progress of renal function and cardiovascular events. The main objective is to analyze whether there are differential risk factors for renal function deterioration between sexes, as well as to analyze cardiovascular events and mortality in this population. In total, 650 patients with a median age of 69 (14) with different grades of chronic kidney disease—G1–G2 (eGFR > 90–60 mL/min/1.73 m2) 50.3%, G3 (eGFR; 59–30 mL/min/1.73 m2) 31.4%, G4 (eGFR; 29–15 mL/min/1.73 m2) 10.8%, and G5 (eGFR < 15 mL/min/1.73 m2) 7.5%—were followed up for 4.7 (0.65) years. Regardless of albuminuria, women lost 0.93 (0.40–1.46) fewer glomerular filtration units per year than men. A total of 17% of the participants experienced rapid deterioration of renal function, 75.2% of whom were men, with differential risk factors between sexes—severe macroalbuminuria > 300 mg/g for men OR [IQ] 2.40 [1.29:4.44] and concomitant peripheral vascular disease 3.32 [1.10:9.57] for women. Overall mortality of 23% was detected (38% of which was due to cardiovascular etiology). We showed that kidney function declined faster in men, with different risk factors compared to women. Patients with T2D and kidney involvement have very high mortality and an important cardiovascular burden. This cohort is proposed as a great tool for scientific collaboration for studies, whether they are focused on T2D, or whether they are interested in comparing differential markers between diabetic and non-diabetic populations.
Accuracy of a Computer-Aided Dynamic Navigation System in the Placement of Zygomatic Dental Implants: An In Vitro Study
Authors: González Rueda Juan Ramón , García Ávila Irene , de Paz Hermoso Víctor Manuel , Riad Deglow Elena , Zubizarreta-Macho Álvaro , Pato Mourelo Jesús , Montero Martín Javier , Hernández Montero Sofía
Published: March 2022

The objective of this in vitro study was to evaluate and compare the accuracy of zygomatic dental implant (ZI) placement carried out using a dynamic navigation system. Materials and Methods: Forty (40) ZIs were randomly distributed into one of two study groups: (A) ZI placement via a computer-aided dynamic navigation system (n = 20) (navigation implant (NI)); and (B) ZI placement using a conventional free-hand technique (n = 20) (free-hand implant (FHI)). A cone-beam computed tomography (CBCT) scan of the existing situation was performed preoperatively to plan the surgical approach for the computer-aided study group. Four zygomatic dental implants were placed in anatomically based polyurethane models (n = 10) manufactured by stereolithography, and a postoperative CBCT scan was performed. Subsequently, the preoperative planning and postoperative CBCT scans were added to dental implant software to analyze the coronal entry point, apical end point, and angular deviations. Results were analyzed using the Student’s t-test. Results: The results showed statistically significant differences in the apical end-point deviations between FHI and NI (p = 0.0018); however, no statistically significant differences were shown in the coronal entry point (p = 0.2617) or in the angular deviations (p = 0.3132). Furthermore, ZIs placed in the posterior region showed more deviations than the anterior region at the coronal entry point, apical end point, and angular level. Conclusions: The conventional free-hand technique enabled more accurate placement of ZIs than the computer-assisted surgical technique. In addition, placement of ZIs in the anterior region was more accurate than that in the posterior region.
Clinical Characteristics of COVID-19 Patients and Application to an Artificial Intelligence System for Disease Surveillance
Authors: Wang Ying-Chuan , Tsai Dung-Jang , Yen Li-Chen , Yao Ya-Hsin , Chiang Tsung-Ta , Chiu Chun-Hsiang , Lin Te-Yu , Yeh Kuo-Ming , Chang Feng-Yee
Published: March 2022

During the coronavirus disease (COVID-19) pandemic, we admitted suspected or confirmed COVID-19 patients to our isolation wards between 2 March 2020 and 4 May 2020, following a well-designed and efficient assessment protocol. We included 217 patients suspected of COVID-19, of which 27 had confirmed COVID-19. The clinical characteristics of these patients were used to train artificial intelligence (AI) models such as support vector machine (SVM), decision tree, random forest, and artificial neural network for diagnosing COVID-19. When analyzing the performance of the models, SVM showed the highest sensitivity (SVM vs. decision tree vs. random forest vs. artificial neural network: 100% vs. 42.86% vs. 28.57% vs. 71.43%), while decision tree and random forest had the highest specificity (SVM vs. decision tree vs. random forest vs. artificial neural network: 88.37% vs. 100% vs. 100% vs. 94.74%) in the diagnosis of COVID-19. With the aid of AI models, physicians may identify COVID-19 patients earlier, even with few baseline data available, and segregate infected patients earlier to avoid hospital cluster infections and to ensure the safety of medical professionals and ordinary patients in the hospital.
Elective Neck Dissection during Salvage Total Laryngectomy: Personal Experience
Authors: Galli Jacopo , Di Cintio Giovanni , Settimi Stefano , Salvati Antonio , Parrilla Claudio , Almadori Giovanni , Paludetti Gaetano
Published: March 2022

The role of elective neck dissection during salvage surgery in patients with a clinically negative neck (cN0) is still discussed. The main objective of this work was to estimate the prevalence and predictive factors of occult neck nodes metastasis; we therefore aimed to evaluate the survival rate and the main oncologic outcomes of cN0 patients who underwent salvage total laryngectomy and elective bilateral neck dissection. In this retrospective observational study, we enrolled 80 cN0 patients affected by recurrent laryngeal cancer and who underwent salvage total laryngectomy and bilateral selective elective neck dissection. Several parameters were collected in order to find prognostic factors; finally, postoperative complications were reviewed and survival analysis was performed. Occult lymph node metastases were reported in 18 out of 80 patients (22.5%). Significant statistical correlation between lymphovascular invasion (p = 0.007), perineural invasion (p = 0.025) and occult nodal metastasis was found. Other variables (glottic subsite of recurrence, clinical T, pathological T, previous chemotherapy) were not significantly predictive of occult nodal metastasis. The 5-year OS, DSS, and RFS were 50.4%, 64.7%, and 63.4%, respectively. In conclusion, our single-institution data on a large cohort of patients, suggest performing routinely elective selective bilateral neck dissection during salvage total laryngectomy in cN0 patients due to the biological attitude of the tumor to spread to cervical nodes, considering an acceptable complications rate.
Cardiopulmonary Exercise Performance and Endothelial Function in Convalescent COVID-19 Patients
Authors: Ambrosino Pasquale , Parrella Paolo , Formisano Roberto , Perrotta Giovanni , D’Anna Silvestro Ennio , Mosella Marco , Papa Antimo , Maniscalco Mauro
Published: March 2022

Background: Endothelial dysfunction has been proposed as the common pathogenic background of most manifestations of coronavirus disease 2019 (COVID-19). Among these, some authors also reported an impaired exercise response during cardiopulmonary exercise testing (CPET). We aimed to explore the potential association between endothelial dysfunction and the reduced CPET performance in COVID-19 survivors. Methods: 36 consecutive COVID-19 survivors underwent symptom-limited incremental CPET and assessment of endothelium-dependent flow-mediate dilation (FMD) according to standardized protocols. Results: A significantly higher FMD was documented in patients with a preserved, as compared to those with a reduced, exercise capacity (4.11% ± 2.08 vs. 2.54% ± 1.85, p = 0.048), confirmed in a multivariate analysis (β = 0.899, p = 0.038). In the overall study population, FMD values showed a significant Pearson’s correlation with two primary CPET parameters, namely ventilation/carbon dioxide production (VE/VCO2) slope (r = −0.371, p = 0.026) and end-tidal carbon dioxide tension (PETCO2) at peak (r = 0.439, p = 0.007). In multiple linear regressions, FMD was the only independent predictor of VE/VCO2 slope (β = −1.308, p = 0.029) and peak PETCO2 values (β = 0.779, p = 0.021). Accordingly, when stratifying our study population based on their ventilatory efficiency, patients with a ventilatory class III-IV (VE/VCO2 slope ≥ 36) exhibited significantly lower FMD values as compared to those with a ventilatory class I-II. Conclusions: The alteration of endothelial barrier properties in systemic and pulmonary circulation may represent a key pathogenic mechanism of the reduced CPET performance in COVID-19 survivors. Personalized pharmacological and rehabilitation strategies targeting endothelial function may represent an attractive therapeutic option.
AaHog1 Regulates Infective Structural Differentiation Mediated by Physicochemical Signals from Pear Fruit Cuticular Wax, Stress Response, and Alternaria alternata Pathogenicity
Authors: Zhang Miao , Wang Tiaolan , Li Yongcai , Bi Yang , Li Rong , Yuan Jing , Xu Wenyi , Prusky Dov
Published: March 2022

The high-osmolarity glycerol response kinase, Hog1, affects several cellular responses, but the precise regulatory role of the Hog1 mitogen-activated protein (MAP) kinase in the differentiation of the infective structure of Alternariaalternata induced by pear cuticular wax and hydrophobicity has not yet clarified. In this study, the AaHog1 in A. alternata was identified and functionally characterized. AaHog1 has threonine-glycine-tyrosine (TGY) phosphorylation sites. Moreover, the expression level of AaHog1 was significantly upregulated during the stages of appressorium formation of A. alternata on the fruit-wax-extract-coated GelBond hydrophobic film surface. Importantly, our results showed that the appressorium and infection hyphae formation rates were significantly reduced in ΔAaHog1 mutants. Furthermore, AaHog1 is beneficial for the growth and development, stress tolerance, virulence, and cell-wall-degrading enzyme activity of A. alternata. These findings may be useful for dissecting the AaHog1 regulatory mechanism in relation to the pathogenesis of A. alternata.
Bioinformatics Analyses Identify the Therapeutic Potential of ST8SIA6 for Colon Cancer
Authors: Ko Chou-Yuan , Chu Tian-Huei , Hsu Ching-Cheng , Chen Hsin-Pao , Huang Shih-Chung , Chang Chen-Lin , Tzou Shiow-Jyu , Chen Tung-Yuan , Lin Chia-Chen , Shih Pei-Chun , Lin Chung-Hsien , Chang Chuan-Fa , Lee Yung-Kuo
Published: March 2022

Sialylation of glycoproteins is modified by distinct sialyltransferases such as ST3Gal, ST6Gal, ST6GalNAc, or ST8SIA with α2,3-, α2,6-, or α2,8-linkages. Alteration of these sialyltransferases causing aberrant sialylation is associated with the progression of colon cancer. However, among the ST8- sialyltransferases, the role of ST8SIA6 in colon cancer remains poorly understood. In this study, we explored the involvement of ST8SIA6 in colon cancer using multiple gene databases. The relationship between ST8SIA6 expression and tumor stages/grades was investigated by UALCAN analysis, and Kaplan–Meier Plotter analysis was used to analyze the expression of ST8SIA6 on the survival outcome of colon cancer patients. Moreover, the biological functions of ST8SIA6 in colon cancer were explored using LinkedOmics and cancer cell metabolism gene DB. Finally, TIMER and TISMO analyses were used to delineate ST8SIA6 levels in tumor immunity and immunotherapy responses, respectively. ST8SIA6 downregulation was associated with an advanced stage and poorly differentiated grade; however, ST8SIA6 expression did not affect the survival outcomes in patients with colon cancer. Gene ontology analysis suggested that ST8SIA6 participates in cell surface adhesion, angiogenesis, and membrane vesicle trafficking. In addition, ST8SIA6 levels affected immunocyte infiltration and immunotherapy responses in colon cancer. Collectively, these results suggest that ST8SIA6 may serve as a novel therapeutic target towards personalized medicine for colon cancer.
Association of TLR4 Rs4986791 Polymorphism and TLR9 Haplotypes with Acute Myeloid Leukemia Susceptibility: A Case-Control Study of Adult Patients
Authors: Banescu Claudia , Tripon Florin , Bojan Anca S. , Trifa Adrian P. , Muntean Carmen , Crauciuc George Andrei , Boglis Alina , Candea Marcela , Lazar Erzsebet , Jimbu Laura , Iancu Mihaela
Published: March 2022

Toll-like receptors (TLRs) have an important role in innate immunity, and single nucleotide polymorphisms (SNPs) of TLR genes influence the risk of developing hematological malignancies. We aimed to evaluate the effect of TLR2 (rs5743708), TLR4 (rs11536889, rs4986790, rs4986791), TLR9 (rs187084, rs352140, rs5743836) on AML risk, the relation between investigated SNPs and somatic mutations, clinical features, and the overall survival of adult AML patients. All mentioned SNPs were genotyped in 511 AML cases and 503 healthy controls. DNMT3A (R882), FLT3 (D835, ITD), and NPM1 mutations’ status were investigated in AML patients. TLR4 rs4986791 was associated with an increased risk of AML under the dominant model (OR = 1.61, 95% CI: 1.001–2.59). Variant genotypes of the TLR4 rs4986790 or rs4986791 were associated with the odds of developing AML in the codominant model (OR = 3.14; 95% CI: 1.12–8.84; p = 0.032). The TLR9 rs5743836 variant genotype was associated with the NPM1 mutation (p = 0.002). The investigated SNPs were not associated with the DNMT3A, FLT3 mutations and had no significant contribution to the hazard of death after adjusting for covariates. Our findings suggest that TLR4 rs4986791 is associated with AML susceptibility. The combined variant genotypes of TLR4 rs4986790 and rs4986791 increase AML risk, the TLR9 C-G-A haplotype may represent a promising approach to predict a person’s risk for developing AML.
Very Low Prostate PET/CT PSMA Uptake May Be Misleading in Staging Radical Prostatectomy Candidates
Authors: Rosenzweig Barak , Haramaty Rennen , Davidson Tima , Lazarovich Alon , Shvero Asaf , Haifler Miki , Gal Jonathan , Golan Shay , Shpitzer Sagi , Hoffman Azik , Nativ Omri , Freifeld Yuval , Zreik Rani , Dotan Zohar A.
Published: March 2022

Purpose: to evaluate a unique subpopulation of radical prostatectomy (RP) candidates with “negative” prostate 68Ga-labeled prostate-specific membrane antigen (PSMA) positron emission tomography (PET) computed tomography (CT) imaging scans and to characterize the clinical implications of misleading findings. Materials and Methods: This case-control retrospective study compared the final histological outcomes of patients with “negative” pre-RP PSMA PET/CT prostate scans (with a prostate maximal standardized uptake value [SUVmax] below the physiologic uptake) to those with an “intense” prostatic tracer uptake (with a SUVmax above the physiologic uptake). The patients underwent an RP between March 2015 and July 2019 in five academic centers. Data on the demographics, comorbidities, prostate-specific antigen (PSA) and rectal exam findings, prior biopsies, imaging results, biopsies, and RP histology results were collected. Results: Ninety-seven of the 392 patients who underwent an RP had PSMA PET/CT imaging preoperatively. Fifty-two (54%) had a “negative” uptake (in the study group), and 45 (46%) had a “positive” uptake (in the control group). Only the lesion size and SUVmax values on the PSMA PET/CT differed between the groups preoperatively. On the histological analysis, only the ISUP score, seminal vesicles invasion, T stage, and positive margin rates differed between the groups (p < 0.05), while 50 (96%) study group patients harbored clinically significant disease (ISUP ≥ 2), with an extra-prostatic disease in 24 (46%), perineural invasion in 35 (67%), and positive lymph nodes in 4 (8%). Conclusions: Disease aggressiveness generally correlated with an intense PSMA uptake on the preoperative PSMA PET/CT, but a subpopulation of patients with clinically significant cancer and aggressive characteristics showed a deceptively weak PSMA uptake. These data raise a concern about the unqualified application of PSMA PET/CT for staging RP candidates.
CoroFinder: A New Tool for Real Time Detection and Tracking of Coronary Arteries in Contrast-Free Cine-Angiography
Authors: Zaffino Paolo , Spadea Maria Francesca , Indolfi Ciro , De Rosa Salvatore
Published: March 2022

Coronary Angiography (CA) is the standard of reference to diagnose coronary artery disease. Yet, only a portion of the information it conveys is usually used. Quantitative Coronary Angiography (QCA) reliably contributes to improving the measurable assessment of CA. In this work, we developed a new software, CoroFinder, able to automatically identify epicardial coronary arteries and to dynamically track the vessel profile in dye-free frames. The coronary tree is automatically segmented by Frangi’s filter in the angiogram’s frames where vessels are contrasted (“template frames”). Afterward, the image similarity among each template frame and the dye-free images is scored by cross-correlation. Finally, each dye-free image is associated with the most similar template frame, resulting in an estimation of vessel contour. CoroFinder allows locating the position of coronary arteries in absence of contrast dye. The developed algorithm is robust to diverse vessel curvatures, variation of vessel widths, and the presence of stenoses. This article describes the newly developed CoroFinder algorithm and the associated software and provides an overview of its potential application in research and for translation to the clinic.
Effect of Flumazenil on Emergence Agitation after Orthognathic Surgery: A Randomized Controlled Trial
Authors: Koo Young Hyun , Choi Geun Joo , Kang Hyun , Jung Yong Hun , Woo Young Cheol , Choi Young-Jun , Baek Chong Wha
Published: March 2022

Flumazenil, a gamma-aminobutyric acid receptor antagonist, can promote arousal even under general anesthesia without the use of benzodiazepines. We hypothesized that flumazenil could promote arousal and reduce emergence agitation in patients undergoing orthognathic surgery with sevoflurane anesthesia. One hundred and two patients were randomly allocated to the control or flumazenil group. Saline or flumazenil was administered at the end of the surgery. The incidence of emergence agitation was measured by using Aono’s four-point scale, with scores of 3 and 4 indicating emergence agitation. The primary outcome was the incidence of emergence agitation. Secondary outcomes included duration of emergence agitation and time intervals between the discontinuation of anesthetics, first response, extubation, and post-anesthesia care-unit discharge readiness. The incidence of emergence agitation was 58.3% and 38.9% in the control and flumazenil groups, respectively, but it was not statistically significant. However, the duration of emergence agitation was shorter in the flumazenil group (p = 0.012). There were no significant differences in the time intervals between the discontinuation of anesthetics, first response, and extubation. Although flumazenil did not reduce the incidence of emergence agitation in patients undergoing orthognathic surgery with sevoflurane anesthesia, it can be considered as an option for awakening patients in terms of improving emergence profiles.
Protected Areas and Rural Depopulation in Spain: A Multi-Stakeholder Perceptual Study
Authors: Rodríguez-Rodríguez David , Larrubia Vargas Remedios
Published: March 2022

Protected areas (PAs) are thought by some to contribute to local wellbeing and socioeconomic development, whereas for others PAs remain a regulatory burden that hampers rural development. Here, we sought to ascertain the perceived causes of rural depopulation and the potential impact of four Natura 2000 sites on the wellbeing and depopulation figures of four protected rural municipalities in Spain that were selected as extreme case studies. We used phone surveys to elicit experts’ views (n = 19) on the topic and convened eight in-person workshops to garner local residents’ insights (n = 40) using structured questionnaires. We complemented perceived wellbeing data from PAs with surveys to residents in neighbouring unprotected municipalities (n = 28). Both experts and workshops’ attendees from protected municipalities overwhelmingly attributed depopulation figures to structural causes linked to transport accessibility, basic service provision and the existence of job opportunities, which they perceived to be unrelated to the PAs’ regulations or management. Local residents did generally not perceive any impact on their collective or individual wellbeing from those PAs, and most who did, expressed a negative impact chiefly due to socioeconomic restrictions. Four-fifths of the experts and half of the workshops’ attendees from protected municipalities, however, expressed that PAs’ administrations could help improve depopulation figures in their towns mainly through promoting tourism and greater compatibility of land uses, including housing and infrastructure development. While the assessed Natura 2000 sites certainly have scope for tourism promotion, their lenient legal regimes make it largely unfeasible to broaden land use compatibility without damaging protected features.
The Impact of Student Teachers’ Pre-Existing Conceptions of Assessment on the Development of Language Assessment Literacy within an LTA Course
Authors: Kvasova Olga
Published: March 2022

The paper proposes the preparation of a new generation of assessment literate teachers. The issues of student assessment literacy and, more specifically, prospective language teacher assessment literacy have not been sufficiently investigated as of yet, although research into the topic seems to have gained momentum. Recent studies state that the assessment literacy of teachers is essentially affected by their pre-existing conceptions of assessments), and teacher education should integrate shaping such conceptions into courses; the process of shaping conceptions is quite long and, because it is time-consuming, it may deter assessment literacy building. The current study explores the conceptions of the assessments shaped by prospective teachers within a general English course. The two major conceptions of assessment, relevant for the framework of teaching general English to second-year student teachers of English, are the understanding of feedback and knowledge of assessment construct and criteria. The findings of the study in this cohort of students of the particular course in language assessment shows that the students’ progress was considerably higher than that of a comparison group in the previous 2020 study. The author suggests two types of AL, i.e., student and prospective teacher assessment literacy.
Obtaining Reliable RT-qPCR Results in Molecular Diagnostics—MIQE Goals and Pitfalls for Transcriptional Biomarker Discovery
Authors: Grätz Christian , Bui Maria L. U. , Thaqi Granit , Kirchner Benedikt , Loewe Robert P. , Pfaffl Michael W.
Published: March 2022

In this review, we discuss the development pipeline for transcriptional biomarkers in molecular diagnostics and stress the importance of a reliable gene transcript quantification strategy. Hence, a further focus is put on the MIQE guidelines and how to adapt them for biomarker discovery, from signature validation up to routine diagnostic applications. First, the advantages and pitfalls of the holistic RNA sequencing for biomarker development will be described to establish a candidate biomarker signature. Sequentially, the RT-qPCR confirmation process will be discussed to validate the discovered biomarker signature. Examples for the successful application of RT-qPCR as a fast and reproducible quantification method in routinemolecular diagnostics are provided. Based on the MIQE guidelines, the importance of “key steps” in RT-qPCR is accurately described, e.g., reverse transcription, proper reference gene selection and, finally, the application of automated RT-qPCR data analysis software. In conclusion, RT-qPCR proves to be a valuable tool in the establishment of a disease-specific transcriptional biomarker signature and will have a great future in molecular diagnostics or personalized medicine.
(±)-((2S,5R)-5-(Acetoxymethyl)tetrahydrofuran-2-yl)methyl Benzoate
Authors: Piccialli Vincenzo
Published: March 2022

In this note we report the synthesis of a doubly acylated cis-THF-diol product synthesised in three steps by the stereoselective RuO4-catalysed oxidative cyclisation of 1,5-hexadiene, followed by benzoylation and acetylation. This substance is one of the substrates chosen to probe a new developed oxidative procedure to transform bis-acylated THF-diols into bis-acylated 1,4-diketones. This new derivative was fully characterised by spectroscopic methods.
Modifier Effect in Silica-Supported FePO4 and Fe-Mo-O Catalysts for Propylene Glycol Oxidation
Authors: Savenko Darya , Salaev Mikhail , Dutov Valerii , Kulinich Sergei , Vodyankina Olga
Published: March 2022

Currently, catalytic processing of biorenewable raw materials into valuable products attracts more and more attention. In the present work, silica-supported FePO4 and Fe-Mo-O catalysts are prepared, their phase composition, and catalytic properties are studied in the process of selective oxidation of propylene glycol into valuable mono- and bicarbonyl compounds, namely, hydroxyacetone and methylglyoxal. A comparative analysis of the main routes of propylene glycol adsorption with its subsequent oxidative conversion into carbonyl products is carried out. The DFT calculations show that in the presence of adsorbed oxygen atom, the introduction of the phosphate moiety to the Fe-containing site strengthens the alcohol adsorption on the catalyst surface with the formation of the 1,2-propanedioxy (–OCH(CH3)CH2O–) intermediate at the active site. The introduction of the molybdenum moiety to the Fe-containing site in the presence of the adsorbed oxygen atom is also energetically favorable, however, the interaction energy is found by 100 kJ/mol higher compared to the case with phosphate moiety that leads to an increase in the propylene glycol conversion while maintaining high selectivity towards C3 products. The catalytic properties of the synthesized iron-containing catalysts are experimentally compared with those of Ag/SiO2 sample. The synthesized FePO4/SiO2 and Fe-Mo-O/SiO2 catalysts are not inferior to the silver-containing catalyst and provide ~70% selectivity towards C3 products, while the main part of propylene glycol is converted into methylglyoxal in contrast to the Ag/SiO2 catalyst featuring the selective transformation of only the secondary C-OH group in the substrate molecule under the studied conditions with the formation of hydroxyacetone. Thus, supported Fe-Mo-O/SiO2 catalysts are promising for the selective oxidation of polyatomic alcohols under low-temperature conditions.
The Photovoltaic Cell Based on CIGS: Principles and Technologies
Authors: Salhi Billel
Published: March 2022

Semiconductors used in the manufacture of solar cells are the subject of extensive research. Currently, silicon is the most commonly used material for photovoltaic cells, representing more than 80% of the global production. However, due to its very energy-intensive and costly production method, other materials appear to be preferable over silicon, including the chalcopyrite-structured semiconductors of the CIS-based family (Cu(In, Ga, Al) (Se, S)2). Indeed, these compounds have bandwidths between 1 eV (CuInSe2) and 3 eV (CuAlS2), allowing them to absorb most solar radiation. Moreover, these materials are currently the ones that make it possible to achieve the highest photovoltaic conversion efficiencies from thin-film devices, particularly Cu(In, Ga)Se2, which is considered the most efficient among all drifts based on CIS. In this review, we focus on the CIGS-based solar cells by exploring the different layers and showing the recent progress and challenges.
Evaluation of Permulgin 3274 as a Material for the Conservation of Beeswax Seals
Authors: Bílková Lenka , Bartl Benjamin , Urbánek Štěpán , Zapletal Martin , Holakovská Libuše , Ďurovič Michal , Hrdlička Zdeněk , Havlín Jakub
Published: March 2022

When treating historical beeswax seals, it seems a natural choice to use materials as similar to the original as possible. The properties of analogous recent materials, however, differ from those of the aged ones, not to mention the fact that the exact composition of the particular sealing wax is usually uncertain. In order to obtain the material of desired properties, recent beeswax is often combined with various additives, including petroleum waxes, or even replaced by mixtures based solely on these products. Within this study, the relevant properties of Permulgin 3274, a ceresin-type wax, were compared with the characteristics of recent and historical beeswaxes. The aim was to evaluate its advantages and limitations, in terms of its possible use for the conservation of beeswax seals. The properties studied were comprised of the chemical composition, thermal properties, mechanical properties, possibilities of colour adjustment and ageing properties. Permulgin 3274′s workability was evaluated by conservators from the National Archives in Prague. The results indicate that, from the technological point of view, Permulgin 3274 could be considered a welcome alternative to the use of traditional conservation mixtures.
Quantification of Bacterial DNA from Infected Human Root Canals Using qPCR and DAPI after Disinfection with Established and Novel Irrigation Protocols
Authors: Weber Marie-Theres , Alkhafaji Yousef , Pioch Anne , Trips Evelyn , Basche Sabine , Dannemann Martin , Kilistoff Alan , Hannig Christian , Sterzenbach Torsten
Published: March 2022

The removal of bacterial infections within the root canal system is still a challenge. Therefore, the cleansing effect of established and new irrigation-protocols (IP) containing silver diamine fluoride (SDF) 3.8% on the whole root canal system was analyzed using quantitative PCR (qPCR) and 4′,6-diamidino-phenylindole-(DAPI)-staining. Extracted human premolars were instrumented up to F2 (ProTaper Gold) under NaCl 0.9% irrigation and incubated with Enterococcus faecalis for 42 days. Subsequently, different ultrasonically agitated IP were applied to the roots: control (no irrigation), 1. NaOCl 3%, EDTA 20%, CHX 2%, 2. NaOCl 3%, EDTA 20%, 3. NaOCl 3%, EDTA 20%, SDF 3.8%, 4. SDF 3.8%, and 5. NaCl 0.9%. One half of the root was investigated fluorescent-microscopically with DAPI. The other half was grinded in a cryogenic mill and the bacterial DNA was quantified with qPCR. The qPCR results showed a statistically significant reduction of bacteria after the application of IP 1, 2, and 3 compared to the control group. While IP 4 lead to a bacterial reduction which was not significant, IP 5 showed no reduction. These data corresponded with DAPI staining. With qPCR a new molecular-biological method for the investigation of the complete root canal system was implemented. The novel IP 3 had an equally good cleansing effect as the already established IP.
Crystallography and Microstructure of 7M Martensite in Ni-Mn-Ga Thin Films Epitaxially Grown on (1 1 2¯ 0)-Oriented Al2O3 Substrate
Authors: Yang Bo , Li Zongbin , Yan Haile , Zhang Yudong , Esling Claude , Zhao Xiang , Zuo Liang
Published: March 2022

Epitaxial Ni-Mn-Ga thin films have been extensively investigated, due to their potential applications in magnetic micro-electro-mechanical systems. It has been proposed that the martensitic phase in the <1 1 0>A-oriented film is much more stable than that in the <1 0 0>A-oriented film. Nevertheless, the magnetic properties, microstructural features, and crystal structures of martensite in such films have not been fully revealed. In this work, the <1 1 0>A-oriented Ni51.0Mn27.5Ga21.5 films with different thicknesses were prepared by epitaxially growing on Al2O3(1 1 2¯ 0) substrate by magnetron sputtering. The characterization by X-ray diffraction technique and transmission electron microscopy revealed that all the Ni51.0Mn27.5Ga21.5 films are of 7M martensite at the ambient temperature, with their Type-I and Type-II twinning interfaces nearly parallel to the substrate surface.
Treatment of Polymeric Films Used for Printed Electronic Circuits Using Ambient Air DBD Non-Thermal Plasma
Authors: Astanei Dragos , Burlica Radu , Cretu Daniel-Eusebiu , Olariu Marius , Stoica Iuliana , Beniuga Oana
Published: March 2022

This study aims to present the properties of the polymeric films after being subjected to DBD plasma treatment in atmospheric conditions. Three different commercial films of polyester (Xerox Inkjet transparencies and Autostat CUS5 Clear film) and polycarbonate (Lexan™ 8010 MC) have been considered for the tests. The surface wettability has been evaluated based on static water contact angle (WCA) for different treatment times varying between 0.2 s and 30 s, the results revealing a maximum WCA decrease compared to a pristine of up to 50% for Xerox films, 75% for Autostat and 70% for Lexan. The persistence of the hydrophilic effect induced by the plasma treatment has also been verified for up to 72 h of storage after treatment, the results indicating a degradation of the treatment effects starting with the first hours after the treatment. The WCA stabilizes to a value inferior to the one corresponding to pristine in the first 24 h after plasma treatment. The adhesion forces, as well as preliminary surface morphology evaluations have been determined for the considered films using atomic force microscopy (AFM). The adhesion forces are increased together with the prolongation of the plasma treatment application time, varying from initial values of 165 nN, 58 nN and 204 nN to around 390 nN, 160 nN and 375 nN for Xerox, Autostat and Lexan films, respectively, after 5 s of DBD treatment. For the considered materials, the results revealed that the plasma treatment determines morphological changes of the surfaces indicating an increase in surface roughness.
The Effect of Whitlockite as an Osteoconductive Synthetic Bone Substitute Material in Animal Bony Defect Model
Authors: Ku Jeong-Kui , Kim Il-hyung , Shim Jung Hee , Kim Yu ha , Kim Baek Hyun , Kim Young-Kyun , Yun Pil-Young
Published: March 2022

This study aimed to evaluate the biomechanical properties in vitro and the bone regeneration of whitlockite (WH) compared with hydroxyapatite (HA) or β-tricalcium phosphate (β-TCP)-based material. We investigated the morphology and phase composition of the bone grafts using a scanning electron microscope and X-ray diffractometer patterns and tested the compressive strength. Four circular defects of 8 mm in diameter were created on the calvaria of twelve rabbits. One defect was left empty, and each of the other defects was filled with WH, HA, and β-TCP. At 4 and 8 weeks, the specimens were harvested to evaluate for the new bone formation and the remaining bone grafts. Regarding the biomechanical properties, the three grafts had a similar micropore size, and WH showed nanopores. The compressive strength of WH was higher than HA and β-TCP without statistical significance. The radiological and histomorphometric analyses demonstrated that the new bone formation was similar among the groups. The remaining bone graft of the WH group was greater than that of the HA and β-TCP groups at 4 weeks (p < 0.05), and the total bone area of the WH, HA, and β-TCP groups was greater than that of the other (p < 0.01). WH has excellent volumetric stability and osteoconductivity compared with HA and β-TCP.
Fatigue Crack Growth Behavior of CP-Ti Cruciform Specimens with Mixed Mode I-II Crack under Biaxial Loading
Authors: Liu Jia-Yu , Bao Wen-Jie , Zhao Jia-Yu , Zhou Chang-Yu
Published: March 2022

Investigations on the fatigue crack growth of commercial pure titanium are carried out with cruciform specimens under different biaxial load ratios (λ = 0, 0.5, and 1) and crack inclination angles (β = 90°, 60°, and 45°) in this paper. Based on the finite element results, the modified solution of stress intensity factors KI and KII for cruciform specimens containing mixed mode I-II crack is obtained by considering crack size, biaxial load ratio, and crack inclination angles. The experimental results show that the maximum tangential stress criterion is fit for the prediction of crack initiation angles for mixed model I-II crack under uniaxial or biaxial loading condition. When the biaxial load ratio increases, the crack propagation angle becomes smaller, and so does the fatigue crack growth rate of mode I crack or mixed mode I-II crack. Based on an equivalent stress intensity factor, a new valid stress intensity factor is proposed to better describe the biaxial fatigue crack growth behavior, which can demonstrate the contribution of mode I and mode II of stress intensity factor.
New Materials and Technologies for Wastewater Treatment
Authors: Limousy Lionel , Thiebault Thomas , Brendle Jocelyne
Published: March 2022

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