bluuebunny/crossref_metadata_2025 huggingface dataset:
Structural networking of the developing brain: from maturation to neurosurgical implications
Authors: De Benedictis Alessandro , Rossi-Espagnet Maria Camilla , de Palma Luca , Sarubbo Silvio , Marras Carlo Efisio
Published: November 2023

Modern neuroscience agrees that neurological processing emerges from the multimodal interaction among multiple cortical and subcortical neuronal hubs, connected at short and long distance by white matter, to form a largely integrated and dynamic network, called the brain “connectome.” The final architecture of these circuits results from a complex, continuous, and highly protracted development process of several axonal pathways that constitute the anatomical substrate of neuronal interactions. Awareness of the network organization of the central nervous system is crucial not only to understand the basis of children’s neurological development, but also it may be of special interest to improve the quality of neurosurgical treatments of many pediatric diseases. Although there are a flourishing number of neuroimaging studies of the connectome, a comprehensive vision linking this research to neurosurgical practice is still lacking in the current pediatric literature. The goal of this review is to contribute to bridging this gap. In the first part, we summarize the main current knowledge concerning brain network maturation and its involvement in different aspects of normal neurocognitive development as well as in the pathophysiology of specific diseases. The final section is devoted to identifying possible implications of this knowledge in the neurosurgical field, especially in epilepsy and tumor surgery, and to discuss promising perspectives for future investigations.
Case report: Recurrent cervical spinal stenosis masquerading as myalgic encephalomyelitis/chronic fatigue syndrome with orthostatic intolerance
Authors: Edwards Charles C. , Edwards Charles C. , Heinlein Scott , Rowe Peter C.
Published: November 2023

Introduction

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex, chronic, multi-system disorder that is characterized by a substantial impairment in the activities that were well tolerated before the illness. In an earlier report, we had described three adult women who met criteria for ME/CFS and orthostatic intolerance, and had congenital or acquired cervical spinal stenosis. All three experienced substantial global improvements in their ME/CFS and orthostatic intolerance symptoms after recognition and surgical treatment of the cervical stenosis. After a several year period of improvement, one of the individuals in that series experienced a return of ME/CFS and orthostatic intolerance symptoms.

Main symptoms and clinical findings

Radiologic investigation confirmed a recurrence of the ventral compression of the spinal cord due to a shift of the disc replacement implant at the involved cervical spinal level.

Therapeutic intervention

Decompression of the spinal cord with removal of the implant and fusion at the original C5-C6 level was once again followed by a similar degree of improvement in function as had been observed after the first operation.

Conclusion

This recapitulation of the outcomes after surgical management of cervical stenosis provides further evidence in support of the hypothesis that cervical spinal stenosis can exacerbate pre-existing or cause new orthostatic intolerance and ME/CFS. Especially for those with refractory symptoms and neurological signs, surgical interventions may offer relief for selected patients with this complex condition.
Investigation of altered spontaneous brain activity in patients with bronchial asthma using the percent amplitude of fluctuation method: a resting-state functional MRI study
Authors: Wang Tao , Huang Xin , Dai Li-xue , Zhan Kang-min , Wang Jun
Published: November 2023

Purpose

To explore the regions of aberrant spontaneous brain activity in asthma patients and their potential impacts using the Percent amplitude of fluctuation (PerAF) analysis method.

Patients and methods

In this study, a total of 31 bronchial asthma (BA) patients were ultimately included, comprising 17 males and 14 females. Subsequently, 31 healthy control subjects (HCS) were recruited, consisting of 17 males and 14 females, and they were matched with the BA group based on age, sex, and educational status. The PerAF analysis technique was employed to study the differences in spontaneous brain activity between the two groups. The SPM12 toolkit was used to carry out a two sample t-test on the collected fMRI data, in order to examine the differences in PerAF values between the asthma patients and the healthy controls. We employed the Montreal Cognitive Assessment (MoCA) scale and the Hamilton Depression Scale (HAMD) to evaluate the cognitive and emotional states of the two groups. Pearson correlation analysis was utilized to ascertain the relationship between changes in the PerAF values within specific brain regions and cognitive as well as emotional conditions.

Results

Compared with the healthy control group, areas of the brain with reduced PerAF in asthma patients included the inferior cerebellum, fusiform gyrus, right inferior orbital frontal gyrus, left middle orbital frontal gyrus, left/right middle frontal gyrus (MFG), dorsal lateral superior frontal gyrus (SFGdl), left superior temporal gyrus (STG), precuneus, right inferior parietal lobule (IPL), and left/right angular gyrus. BA patients exhibit mild cognitive impairments and a propensity for emotional disturbances. Furthermore, the perAF values of the SFGdl region are significantly positively correlated with the results of the MoCA cognitive assessment, while negatively correlated with the HAMD evaluation.

Conclusion

Through the application of PerAF analysis methods, we discovered that several brain regions in asthma patients that control the amplitude of respiration, vision, memory, language, attention, and emotional control display abnormal changes in intrinsic brain activity. This helps characterize the neural mechanisms behind cognitive, sensory, and motor function impairments in asthma patients, providing valuable insights for potential therapeutic targets and disease management strategies.
Impact of a simplified treatment protocol for moderate acute malnutrition with a decentralized treatment approach in emergency settings of Niger
Authors: Sánchez-Martínez Luis Javier , Charle-Cuéllar Pilar , Gado Abdoul Aziz , Dougnon Abdias Ogobara , Sanoussi Atté , Ousmane Nassirou , Lazoumar Ramatoulaye Hamidou , Toure Fanta , Vargas Antonio , Hernández Candela Lucía , López-Ejeda Noemí
Published: November 2023

Introduction

Of the 45.4 million children under five affected by acute malnutrition in the world, the majority (31.8 million) are affected by moderate acute malnutrition (MAM). Its treatment is particularly complex in emergency settings such as the Diffa region in Niger. This study aims to evaluate the effectiveness and coverage of a simplified treatment protocol with Community Health Workers (CHWs) as treatment providers.

Methods

This study is a non-randomized controlled trial. The control group (n = 181) received the standard protocol currently used in country, delivered by nursing staff only in health centres and health posts, while the intervention group (n = 483) received the simplified protocol which included nursing at health centres and CHWs at health post as treatment providers.

Results

The recovery rate was higher in the simplified protocol group (99.6% vs. 79.56%, p < 0.001) recording lower time to recover and higher anthropometric gain. Treatment coverage in the intervention group increased from 28.8% to 84.9% and reduced in the control group (25.3% to 13.6%). No differences were found in the recovery rate of children treated by CHWs and nursing staff.

Conclusion

The outcomes using the simplified protocol exceeded humanitarian requirements and demonstrated improvements compared to the standard protocol showing that the simplified protocol could be safely provided by CHWs in an emergency context. Further research in other contexts is needed to scale up this intervention.
Evaluating the effectiveness of the Ministry of Health restriction policy on seasonal antibiotic consumption trends in Saudi Arabia, 2016–2020
Authors: Alzahrani Khaloud O. , Alshahrani Saeed M. , Alajel Sulaiman M.
Published: November 2023

Background: Understanding antibiotic consumption patterns over time is essential to optimize prescribing practices and minimizing antimicrobial resistance. This study aimed to determine whether the antibiotics restriction policy launched by the Saudi Ministry of Health in April 2018 has impacted antibiotic use by assessing changes and seasonal variations following policy enforcement.

Methods: Quarterly sales data of J01 antibacterial for systemic use in standard units were obtained from the IQVIA-MIDAS database, spanning from the first quarter of 2016 to the last quarter of 2020. Antibiotics consumption was measured in defined daily doses per 1,000 inhabitant per day- in a quarter (DDDdq). A comparative analysis of antibiotic consumption pre- and post-policy periods introduction was conducted by computing the average consumption values for each period. Statistical comparison of the mean differences between the two periods were then made using independent samples t-test, Mann-Whitney U Test where needed. Time series analysis was employed to estimate the projected antibiotic consumption in the post-policy period if the restriction policy had not been implemented, which was then compared to actual consumption values to evaluate the effectiveness of the restriction policy.

Results: During the pre-policy, there were seasonal trends of the total and oral antibiotic consumption through quarters, with higher consumption observed in the first and fourth quarters. In contrast, parenteral antibiotic consumption did not appear to follow a clear seasonal pattern. Following the restriction policy, there was a significant reduction in total and oral antibiotic use, with mean reductions of −96.9 DDDdq (p-value = 0.002) and −98 DDDdq (p-value = 0.002), respectively. Conversely, a significant increase in parenteral antibiotic consumption was observed with a mean increase of +1.4 DDDdq (p-value < 0.0001). The comparison between the forecasted and actual models showed that the actual antibiotics consumption for total, oral, and parenteral were lower than the corresponding forecasted values by 30%, 31%, and 34%, respectively.

Conclusion: Overall, our analysis of antibiotics consumption from 2016 to 2020 displays great success for the policy implemented by the Saudi Ministry of Health in significantly reducing the total and oral use of antibiotics. However, future studies are needed to explore the increased consumption of the parenteral antibiotics as well as the persistent high consumption patterns during the fall and winter months even after the implementation of the restriction policy.
Methylsulfonylmethane ameliorates metabolic-associated fatty liver disease by restoring autophagy flux via AMPK/mTOR/ULK1 signaling pathway
Authors: Han Daewon , Kim Deokryong , Kim Haeil , Lee Jeonga , Lyu Jungmook , Kim Jong-Seok , Shin Jongdae , Kim Jeong Sig , Kim Do Kyung , Park Hwan-Woo
Published: November 2023

Introduction: Metabolism-associated fatty liver disease (MAFLD) is a global health concern because of its association with obesity, insulin resistance, and other metabolic abnormalities. Methylsulfonylmethane (MSM), an organic sulfur compound found in various plants and animals, exerts antioxidant and anti-inflammatory effects. Here, we aimed to assess the anti-obesity activity and autophagy-related mechanisms of Methylsulfonylmethane.

Method: Human hepatoma (HepG2) cells treated with palmitic acid (PA) were used to examine the effects of MSM on autophagic clearance. To evaluate the anti-obesity effect of MSM, male C57/BL6 mice were fed a high-fat diet (HFD; 60% calories) and administered an oral dose of MSM (200 or 400 mg/kg/day). Moreover, we investigated the AMP-activated protein kinase (AMPK)/mechanistic target of rapamycin complex 1 (mTORC1)/UNC-51-like autophagy-activating kinase 1 (ULK1) signaling pathway to further determine the underlying action mechanism of MSM.

Results: Methylsulfonylmethane treatment significantly mitigated PA-induced protein aggregation in human hepatoma HepG2 cells. Additionally, Methylsulfonylmethane treatment reversed the PA-induced impairment of autophagic flux. Methylsulfonylmethane also enhanced the insulin sensitivity and significantly suppressed the HFD-induced obesity and hepatic steatosis in mice. Western blotting revealed that Methylsulfonylmethane improved ubiquitinated protein clearance in HFD-induced fatty liver. Remarkably, Methylsulfonylmethane promoted the activation of AMPK and ULK1 and inhibited mTOR activity.

Conclusion: Our study suggests that MSM ameliorates hepatic steatosis by enhancing the autophagic flux via an AMPK/mTOR/ULK1-dependent signaling pathway. These findings highlight the therapeutic potential of MSM for obesity-related MAFLD treatment.
Spatial mapping of ectonucleotidase gene expression in the murine urinary bladder
Authors: Aresta Branco Mafalda S. L. , Perrino Brian A. , Mutafova-Yambolieva Violeta N.
Published: November 2023

Purinergic signaling is important for normal bladder function, as it is thought to initiate the voiding reflex and modulate smooth muscle tone. The availability of adenine nucleotides and nucleosides (aka purines) at receptor sites of various cell types in the bladder wall is regulated by ectonucleotidases (ENTDs). ENTDs hydrolyze purines such as adenosine 5′-triphosphate (ATP) and adenosine 5′-diphosphate (ADP) with varying preference for the individual substrate. Therefore, the end effect of extracellular purines may depend significantly on the type of ENTD that is expressed in close proximity to the target cells. ENTDs likely have distinct cellular associations, but the specific locations of individual enzymes in the bladder wall are poorly understood. We used RNAscope™, an RNA in situ hybridization (ISH) technology, to visualize the distribution and measure the levels of gene expression of the main recognized ectonucleotidases in large high-resolution images of murine bladder sections. The relative gene expression of ENTDs was Entpd3 > Alpl >> Enpp1 = Entpd2 >> Enpp3 > Entpd1 (very low to no signal) in the urothelium, Entpd1 ≥ Entpd2 >> Enpp3 > Enpp1 = Alpl ≥ Nt5e (very low to no signal) in the lamina propria, and Entpd1 >> Nt5e = Entpd2 >> Enpp1 > Alpl = Enpp3 in the detrusor. These layer-specific differences might be important in compartmentalized regulation of purine availability and subsequent functions in the bladder wall and may explain reported asymmetries in purine availability in the bladder lumen and suburothelium/lamina propria spaces.
Underpinning beneficial maize response to application of minimally processed homogenates of red and brown seaweeds
Authors: Vaghela Pradipkumar , Gandhi Grishma , Trivedi Khanjan , Anand K. G. Vijay , Chavda Dhruvil , Manna Moutusi , Seth Tanmaye , Seth Abhiram , Shanmugam Munisamy , Ghosh Arup
Published: November 2023

Sap from the fresh seaweed Kappaphycus alvarezii (KA) has been reported to improve crop growth, quality, and stress alleviation. However, limited studies are reported for the minimally processed aqueous homogenates (MPHs) derived from dry seaweeds. The present investigation was envisaged to characterize the MPHs from the red seaweed KA and a brown seaweed Sargassum wightii (SW) and also assess the effect of foliar application on maize (Zea mays) crop performance when applied alone or in proportions ranging from 0% to 100%. Two doses (0.35% and 0.7%) were compared with control. Both the MPHs contained several compounds like retronecine, tyrosyl-glycine, hexyl 2-furoate, 1-phosphatidyl-1D-myo-inositol, 12-(2,3-dihydroxycyclopentyl)-2-dodecanone, and trihomomethionine and many others that have known bioactivity for enhancing plant growth and providing stress tolerance. Both doses of MPHs enhanced crop growth and yield; however, the best response was in general observed at a lower dose. The MPH of SW at 100% gave the highest seed yield at a lower dose, which was also on par with that obtained under a lower dose of 100% KA. Other combinations, 80:20 and 40:60 KA : SW, were also found to give comparable yields. The highest dose of 100% MPH of SW was found on par with control, a phenomenon that was investigated in detail with respect to metabolites and antioxidant profile in leaves as well as membrane modeling. Higher ROS and certain sugar and organic acids were observed in 100% MPH of SW at a higher dose, although none of the antioxidant enzymes were significantly affected, nor was there any change in membrane characteristics of the leaf with respect to control as well as lower dose. Improvements in the seed yield were attributed to improved photosynthate production on account of higher dry matter accumulation in the MPH-treated plants, which may also be attributed to the presence of bioactive compounds in the biostimulants. In the future, it is imperative to direct scientific investigations towards the quantification and identification of the most effective concentrations of these compounds within MPHs to optimize plant responses. The study indicated the beneficial use of the MPHs towards increasing crop production by employing optimum dose as foliar spray to crops.
Genomic regions involved in the control of 1,000-kernel weight in wild relative-derived populations of durum wheat
Authors: Jabbour Yaman , Hakim Mohammad Shafik , Al-Yossef Abdallah , Saleh Maysoun M. , Shaaban Ahmad Shams Al-Dien , Kabbaj Hafssa , Zaïm Meryem , Kleinerman Charles , Bassi Filippo M.
Published: November 2023

Terminal drought is one of the most common and devastating climatic stress factors affecting durum wheat (Triticum durum Desf.) production worldwide. The wild relatives of this crop are deemed a vast potential source of useful alleles to adapt to this stress. A nested association mapping (NAM) panel was generated using as a recurrent parent the Moroccan variety ‘Nachit’ derived from Triticum dicoccoides and known for its large grain size. This was recombined to three top-performing lines derived from T. dicoccoides, T. araraticum, and Aegilops speltoides, for a total of 426 inbred progenies. This NAM was evaluated across eight environments (Syria, Lebanon, and Morocco) experiencing different degrees of terminal moisture stress over two crop seasons. Our results showed that drought stress caused on average 41% loss in yield and that 1,000-kernel weight (TKW) was the most important trait for adaptation to it. Genotyping with the 25K TraitGenetics array resulted in a consensus map of 1,678 polymorphic SNPs, spanning 1,723 cM aligned to the reference ‘Svevo’ genome assembly. Kinship distinguished the progenies in three clades matching the parent of origin. A total of 18 stable quantitative trait loci (QTLs) were identified as controlling various traits but independent from flowering time. The most significant genomic regions were named Q.ICD.NAM-04, Q.ICD.NAM-14, and Q.ICD.NAM-16. Allelic investigation in a second germplasm panel confirmed that carrying the positive allele at all three loci produced an average TKW advantage of 25.6% when field-tested under drought conditions. The underlying SNPs were converted to Kompetitive Allele-Specific PCR (KASP) markers and successfully validated in a third germplasm set, where they explained up to 19% of phenotypic variation for TKW under moisture stress. These findings confirm the identification of critical loci for drought adaptation derived from wild relatives that can now be readily exploited via molecular breeding.
Elevated high sensitive C-reactive protein in fibromyalgia
Authors: Beiner Eva , Brenner Miguel Sergio , Friederich Hans-Christoph , Tesarz Jonas ,   PerPAIN Consortium
Published: November 2023

Introduction

Fibromyalgia syndrome (FMS) is a complex chronic pain condition characterized by widespread pain and tenderness, fatigue, and sleep disturbances. Currently, factors contributing to FMS are considered to be multifactorial, and the involvement of inflammatory processes is a question of debate.

Objective

The aims of this study were (1) to assess whether serum concentrations of high-sensitivity C-reactive protein (hsCRP) differ between individuals diagnosed with FMS and pain-free controls, (2) to determine whether these differences are associated with clinical symptoms, and (3) to explore whether the observed differences can be explained by specific covariates such as age, weight, and smoking status.

Methods

An ANOVA was applied to identify differences of hsCRP levels between FMS and pain-free controls and an analysis of covariance (ANCOVA) was performed to investigate the dependencies of hsCRP with respect to covariates. To assess the reliability of our findings, we also utilized a Bayesian robust estimation model to determine the level of confidence associated with our results.

Results

The results showed that individuals with FMS had higher hsCRP levels compared to healthy controls [F(1,106) = 8.802, p < 0.001] and that higher hsCRP levels were significant correlated with a higher symptom burden (r = 0. 287, p = 0.008) and more tender points (r = 0.307, p = 0.005). Further, hsCRP levels were significantly associated with weight (η2 = 0.154, p < 0.001), but independent of age (η2 = 0.005, p = 0.42), smoking status (η2 = 0.002, p = 0.623), or gender (η2 = 0.0045, p = 0.437), which resulted in an insignificant group effect between FMS and controls (η2 = 0.029, p = 0.052), even after controlling for covariates.

Conclusion

In conclusion, this study provides evidence that sub-inflammatory processes correlate with clinical symptoms, which can be partly attributed to differences in weight, but cannot be fully explained by them. Further research is needed to elucidate the mechanisms underlying the association between hsCRP and FMS and to explore the potential therapeutic implications of targeting hsCRP in the management of FMS.
Towards a comprehensive approach to mentalization-based treatment for children with autism: integrating attachment, neurosciences, and mentalizing
Authors: Costa-Cordella Stefanella , Soto-Icaza Patricia , Borgeaud Karin , Grasso-Cladera Aitana , Malberg Norka T.
Published: November 2023

Autism spectrum disorder (ASD) is diagnosed based on socio-communicative difficulties, which are believed to result from deficits in mentalizing, mainly evidenced by alterations in recognizing and responding to the mental states of others. In recent years, efforts have been made to develop mentalization-based treatment (MBT) models for this population. These models focus on enhancing individuals’ ability to understand and reflect on their own mental states, as well as those of others. However, MBT approaches for people with ASD are limited by their existing theoretical background, which lacks a strong foundation grounded in neuroscience-based evidence properly integrated with attachment, and mentalizing. These are crucial aspects for understanding psychological processes in autism, and as such, they play a pivotal role in shaping the development of tailored and effective therapeutic strategies for this specific population. In this paper we review evidence related to the neurobiological, interpersonal, and psychological dimensions of autism and their implications for mentalizing processes. We also review previous mentalization-based frameworks on the psychosis continuum to provide a comprehensive understanding of attachment, neurobiology, and mentalization domains in therapeutic approaches for autism. After presenting a synthesis of the literature, we offer a set of clinical strategies for the work with children with autism. Finally, we provide recommendations to advance the field towards more robust models that can serve as a basis for evidence-based therapeutic strategies.
Engaging “unusual suspects” in climate action: cultural affordances for diverse competences and improvised identities
Authors: Heiskanen Eva
Published: November 2023

The demonstrated urgency of the climate crisis would require mobilization by a larger and more diverse set of participants than those usually recognized as environmental activists. Hence this article asks: (1) What conditions enable unlikely participants (such as men working in manual occupations) to engage in and identify with a climate movement? And (2) what is it about the relationship between participants’ biographies, the practices of the climate movement and the interaction between them that allows – or affords – such identification to occur? I draw on an approach to identity formation as situated practice, i.e., as occurring in situations where social relations are enacted while drawing on the individual experience and shared understandings that participants bring to the situation. Based on fieldwork in Finnish municipalities that have committed to climate neutrality, I find that the conditions for engagement depend on socio-cultural affordances for engaging in climate action, which (1) accept and welcome participants’ life histories and lifestyles (2) build on and respect participants’ competences and multiple forms of expertise, (3) engage participants in practices that are familiar enough not to produce anxiety but stimulating enough to be fun, and (4) produce small but visible achievements that are acknowledged as such by both participants and onlookers. The current study contributes to previous research arguing for a more populist approach to climate policy by emphasizing existing competences and embodied practices as an avenue for engagement in climate action.
Artificial intelligence in remote sensing geomorphology—a critical study
Authors: Mall Urs , Kloskowski Daniel , Laserstein Philip
Published: November 2023

Planetary geomorphological maps over a wide range of spatial and temporal scales provide important information on landforms and their evolution. The process of producing a geomorphological map is extremely time-consuming and maps are often difficult to reproduce. The success of deep learning and machine learning promises to drastically reduce the cost of producing these maps and also to increase their reproducibility. However, deep learning methods strongly rely on having sufficient ground truth data to recognize the wanted surface features. In this study, we investigate the results from an artificial intelligence (AI)–based workflow to recognize lunar boulders on images taken from a lunar orbiter to produce a global lunar map showing all boulders that have left a track in the lunar regolith. We compare the findings from the AI study with the results found by a human analyst (HA) who was handed an identical database of images to identify boulders with tracks on the images. The comparison involved 181 lunar craters from all over the lunar surface. Our results show that the AI workflow used grossly underestimates the number of identified boulders on the images that were used. The AI approach found less than one fifth of all boulders identified by the HA. The purpose of this work is not to quantify the absolute sensitivities of the two approaches but to identify the cause and origin for the differences that the two approaches deliver and make recommendations as to how the machine learning approach under the given constraints can be improved. Our research makes the case that despite the increasing ease with which deep learning methods can be applied to existing data sets, a more thorough and critical assessment of the AI results is required to ensure that future network architectures can produce the reliable geomorphological maps that these methods are capable of delivering.
When a bad day at the golf course is a bad day at the office: occupational stressors, institutional supports, and the mental health of NCAA golf coaches
Authors: Upenieks Laura , Ryan Brendan M. , Carson Howie J.
Published: November 2023

This study examined the mental health of NCAA collegiate golf coaches. Utilizing the person-environment fit theory and previous literature on coaches' well-being, this study examined four outcomes among 48 participants, namely: depressive and anxiety symptoms, burnout, and job turnover intentions. Results suggested that coaching stressors (e.g., administrative tasks, practice plans, pressure to win) only associate with greater burnout. More consistent evidence showed that workplace stress (e.g., lack of control and autonomy, poor work-family balance) associated with higher levels of all outcomes. Finally, greater perceived organizational support had a beneficial association with each outcome. The findings of the current study suggest golf coaches are at risk of mental health problems because of the stressors of this job. Taken as a whole, athletic departments, coaches, and student-athletes must reconsider norms that overemphasize performance and underemphasize self-care and work-life balance.
Homer 1 genotype AA variant relates to congenital splay leg syndrome in piglets by repressing Pax7 in myogenic progenitors
Authors: Schumacher Toni , Reyer Henry , Maak Steffen , Röntgen Monika
Published: November 2023

Introduction

Porcine congenital splay leg syndrome (PCS) is a major birth defect in piglets, resulting in lameness and high mortality rates. The multifactorial pathogenesis of PSC is not well understood but includes a polygenic inheritance.

Methods

Here, in addition to morphological investigations, we characterized the expression of myogenic genes and functional (proliferation and differentiation) properties of myogenic precursor/satellite cells (SATCs) in 1 day-old PCS piglets, non-affected littermates (LCs), and piglets from PCS-free healthy litters (HCs). In addition, PCS phenotypes were related to the SNP Homer1_rs325197091 within the Homer1 locus, which has been identified as a potential hereditary cause of PCS.

Results and discussion

Samples from musculus semitendinosus (ST) of PCS piglets had a higher proportion of type II fibers, reflecting myofiber immaturity. In addition, myofiber atrophy, a lower number of myonuclei per fiber (ST), and a higher apoptotic activity (in ST and longissimus dorsi muscle; LD) were found in the PCS group. A higher proportion of cycling committed myoblasts (Pax7+/Ki67+ cells) occurred in samples from PCS-affected piglets, and on the other hand, the mRNA expression of genes involved in differentiation (muscle differentiation 1; MyoD, myogenin; MyoG) was repressed compared with HCs. Cultured SATCs from PCS-affected animals showed a temporal shift in peak expression of Pax7, MyoD, and MyoG toward days 3 and 4 of their 7 days differentiation regime. In vitro experiments with isolated SATCs confirmed the lower differentiation potential and the delayed progression of the myogenic processes in cells from piglets with PCS phenotype. In addition, Pax7 and desmin were differently expressed in Homer1_rs325197091 genotype variants (GG, GA, and AA). Both genes showed the lowest expression in the homozygous AA-variant, which was most frequently found in PCS-affected animals. The homozygous AA-variant was also associated with lower expression of the truncated Homer1-subtype 205. Thus, we hypothesize that in PCS, the balance between Homer1 proteins and its signaling functions is changed in a way detrimental to the myogenic differentiation program. Our results demonstrated direct negative effects of the Homer1 AA genotype on Pax7 expression, but the exact mode of action still needs to be elucidated.
Global Outward Foreign Direct Investment Network: Perspectives for Knowledge Transfer from Host Countries to Home Countries by MNEs
Authors: Sultana Nasrin , Turkina Ekaterina
Published: November 2023

The possession of diverse knowledge is vital for countries to maintain competitive advantages as new technologies and other disruptive changes emerge. Foreign Direct Investment (FDI) has become an important instrument to access knowledge and innovation available in other countries with the increasing trend of globalization. However, little is known about how the knowledge base of a country can be enriched in the context of knowledge sourcing through FDI, drawing upon general contexts of knowledge and innovation in countries. By adopting the social network analysis methodology to model and analyze the global outward FDI network for the period 2009–2016 and conducting longitudinal regression analyses, this study reveals that the global outward FDI network has a core–periphery structure, the network centrality position of a country is positively and significantly associated with the knowledge base of the country, and the absorptive capacity of a country positively moderates the association. Equipped with empirical evidence to support our theory, we develop the K-NACK framework to understand the context of knowledge sourcing through FDI. This empirical research article offering novel analysis and a theoretical and conceptual framework will be useful for policymakers at firms and in individual countries to find advanced as well as complementary knowledge from host countries to improve the knowledge base in home countries.
Effects of High-Lipid Dietary Protein Ratio on Growth, Antioxidant Parameters, Histological Structure, and Expression of Antioxidant- and Immune-Related Genes of Hybrid Grouper
Authors: Huang Weibin , Liu Hao , Yang Shipei , Zhou Menglong , Zhang Shuang , Tan Beiping , Yang Yuanzhi , Zhang Haitao , Xie Ruitao , Dong Xiaohui
Published: November 2023

The hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus) is a new species of grouper crossed from giant grouper (E. lanceolatus) as the male parent and brown-marbled grouper (E. fuscoguttatus) as the female parent. We hypothesized that optimal levels of dietary protein may benefit liver function. High-lipid diets are energetic feeds that conserve protein and reduce costs, and are a hot topic in aquaculture today. Therefore, the objective of the research is to investigated the effects of dietary protein level in high-lipid diets on serum and liver biochemistry, liver histology, and liver immune and antioxidant indexes and gene mRNA expression of the juvenile hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus). Six iso-lipidic (161 g/kg) diets were formulated containing graded levels of protein (510 as control, 480,450, 420, 390 and 360 g/kg). Each treatment consisted of three replicates and 30 fish (6.70 ± 0.02 g) in one replicate. After an 8-week feeding experiment, the results indicated the following: (1) With the decreasing of dietary protein level, the specific growth rate (SGR) of groupers increased gradually and then decreased; SGRs of the 390 and 360 g/kg groups were significantly lower than other groups (p < 0.05). (2) In terms of serum and liver, the activity of antioxidant enzymes such as catalase (CAT) and superoxide dismutase (SOD), and the total antioxidant capacity (T-AOC) content, and the activity of immune enzymes such as lysozyme (LYS) and immunoglobulin (IgM) was significantly increased under the appropriate protein level. (3) Based on liver histology, we know that high or low dietary protein levels cause liver damage. (4) Dietary protein levels can significantly affect the mRNA expression levels of an anti-inflammatory factor gene (tgfβ), pro-inflammatory factor genes (il6, il8), heat shock proteins, and antioxidant and immune genes (hsp70 and hsp90, gpx, nrf2, keap1). It is concluded that the appropriate protein level can promote the growth performance of groupers, improve antioxidant activity and immune enzyme activity in serum and liver, and enhance the expression of immune genes.
Optimizing Faulting Prediction for Rigid Pavements Using a Hybrid SHAP-TPE-CatBoost Model
Authors: Xiao Wei , Wang Changbai , Liu Jimin , Gao Mengcheng , Wu Jianyang
Published: November 2023

Faulting refers to the common and significant distress in Jointed Plain Concrete Pavement (JPCP), which has an adverse impact on the pavement roughness. Nevertheless, the existing fault prediction models continue to heavily rely on conventional linear regression techniques or basic machine learning approaches, which leaves room for improvement in training efficiency and interpretability. To enhance training efficiency and accuracy, this study developed five novel faulting prediction models. These models are based on five basic machine learning algorithms: Random Forest (RF), Additive Boosting (AdaBoost), Gradient Boosting Decision Tree (GBDT), Light Gradient Boosting Machine (LightGBM), and Categorical Boost (CatBoost), combined with the tree-structured Parzen estimator (TPE). The five models are TPE-RF, TPE-AdaBoost, TPE-GBDT, TPE-LightGBM, and TPE-CatBoost. In addition to selecting the best-performing model, this study incorporated the Shapley Additive Explanation (SHAP) technique and developed TPE-SHAP-CatBoost to improve the interpretability of the model’s predictions. The process involved extracting historical data on pavement performance, including 17 variables, from the Long-Term Pavement Performance (LTPP) database for 160 instances of observation. Firstly, the Boruta method was used to identify the final set of input variables. Secondly, the TPE technique, which is a Bayesian optimization method, was applied to automatically select the optimal hyperparameters for the base models. Finally, SHAP was used to provide both global and local explanations of the model’s outputs. The results indicate that the TPE-CatBoost model achieves the highest accuracy with an R2 value of 0.906. Furthermore, the TPE-SHAP-CatBoost model identified the primary factors influencing faulting by incorporating SHAP and provided explanations of the model’s results at both the global and local levels. These research findings highlight the ability of the proposed model to accurately predict faulting, providing precise and interpretable guidance for pavement maintenance while reducing workload for pavement engineers in data collection and management.
Binomial Sum Relations Involving Fibonacci and Lucas Numbers
Authors: Adegoke Kunle , Frontczak Robert , Goy Taras
Published: November 2023

In this paper, we provide a first systematic treatment of binomial sum relations involving (generalized) Fibonacci and Lucas numbers. The paper introduces various classes of relations involving (generalized) Fibonacci and Lucas numbers and different kinds of binomial coefficients. We also present some novel relations between sums with two and three binomial coefficients. In the course of exploration, we rediscover a few isolated results existing in the literature, commonly presented as problem proposals.
Reduced Infestation Levels of Lepeophtheirus salmonis in Atlantic Salmon (Salmo salar) following Immersion Exposure to Probiotic Aliivibrio spp.
Authors: Steen Dobloug Marius , Skagen-Sandvik Camilla , Evensen Øystein , Gadan Koestan , Bakke Marit Jørgensen , Sørum Henning , Salonius Kira
Published: November 2023

Salmon lice (Lepeophtheirus salmonis) constitute a major challenge during the production of farmed Atlantic salmon in Norway. Preventive measures are considered to have a higher impact on sustainable control than lice treatment. Therefore, the studies presented here aimed to document the preventive effects of probiotic Aliivibrio spp. on lice infestation in experimental challenges. A reduction in salmon lice attachment success (58–65%) was observed in two separate aquarium trials, where Atlantic salmon were exposed to different compositions of Aliivibrio species 91 and 155 days prior to lice challenge. In a third trial, no difference in attachment was observed in groups exposed to probiotics 58 days prior to lice challenge compared to controls. However, a relative reduction in lice counts was seen on movable stages later in the trial. High levels of probiotic bacteria had no impact on lice viability in an in vitro bioassay on the preadult life stage; thus, the mechanism behind the preventative effect remains unknown. In conclusion, probiotic Aliivibrio bacteria can likely be used as a preventive tool to reduce salmon louse infestations in the salmon industry. The mechanism is still unknown, and this novel approach to lice control warrants further investigation to understand its optimal use and potential.
Reshaping the Digital Twin Construct with Levels of Digital Twinning (LoDT)
Authors: Vieira João , Poças Martins João , de Almeida Nuno Marques , Patrício Hugo , Morgado João
Published: November 2023

While digital twins (DTs) have achieved significant visibility, they continue to face a problem of lack of harmonisation regarding their interpretation and definition. This diverse and interchangeable use of terms makes it challenging for scientific activities to take place and for organisations to grasp the existing opportunities and how can these benefit their businesses. This article aims to shift the focus away from debating a definition for a DT. Instead, it proposes a conceptual approach to the digital twinning of engineering physical assets as an ongoing process with variable complexity and evolutionary capacity over time. To accomplish this, the article presents a functional architecture of digital twinning, grounded in the foundational elements of the DT, to reflect the various forms and levels of digital twinning (LoDT) of physical assets throughout their life cycles. Furthermore, this work presents UNI-TWIN—a unified model to assist organisations in assessing the LoDT of their assets and to support investment planning decisions. Three case studies from the road and rail sector validate its applicability. UNI-TWIN helps to redirect the discussion around DTs and emphasise the opportunities and challenges presented by the diverse realities of digital twinning, namely in the context of engineering asset management.
The Correspondence between Large Pressure Fluctuations and Runway Wind Shear: The Event on 12 December 2019 at Songshan Airport, Taipei
Authors: Pu Chin-Piao , Wang Chung-Chieh
Published: November 2023

In this study, the association of large pressure fluctuations (LPFs) ≥ 0.2 hPa and runway wind shear (RWS) ≥ 12 kt at the Songshan Airport in Taipei, Taiwan, during the event on 12 December 2019 with strong northeasterly winds are analyzed. The goal of the study is to demonstrate that the two phenomena exhibited close correspondence, and the former (LPFs) measured using a single barometer can be useful to detect the latter (RWS), which relies on the low-level wind-shear alert system (LLWAS) at the present time. Concentrated before 1200 UTC and especially during 0100-0800 UTC, both LPFs (52 times) and RWS (62 times) over the runway exhibited close association, and one rarely occurred more than 15–20 min apart in time from the other. Using the 2 × 2 contingency table and categorical scores, our results for LPFs and RWS to both occur at least once or five times within the same hour also suggest high accuracy rates of ≥80% and low miss rate and false alarm ratio of both < 10%, respectively. The two variables are also tested to be statistically dependent on each other to a high confidence level of 95–97.5%. Thus, using LPFs as an auxiliary or additional method to detect RWS at airports appears to be reasonable and feasible. At small and remote airports where the LLWAS is not yet available, this method also provides a good and less expensive alternative and can be helpful to the overall improvement of air traffic safety around the world.
Implementation of Digital Twin and Real Production System to Address Actual and Future Challenges in Assembly Technology
Authors: Christ Lukas , Milloch Elías , Boshoff Marius , Hypki Alfred , Kuhlenkötter Bernd
Published: November 2023

Increasing volatility in manufacturing and rising sustainability requirements demand more efficient processes in production, especially in employee qualification and engineering during development and on-site adjustments before and after the start of production. One possible solution is using digital twins for virtual commissioning, which can speed up engineering processes, qualify employees, and save valuable resources. To solve these challenges, it is necessary to identify promising approaches for using the digital twin and virtual commissioning. Furthermore, creating an environment where these approaches can be optimally explored is essential. This paper presents promising research approaches and demonstrates the development of an assembly process and a production system with a digital twin designed to explore these aspects. The presented system is an interlinked production system for assembling an actual industrial product. It includes different levels of human–robot interaction and automation, which can be implemented virtually in the digital twin.
The Role of the NF-kB Pathway in Intracranial Aneurysms
Authors: Blaj Laurentiu Andrei , Cucu Andrei Ionut , Tamba Bogdan Ionel , Turliuc Mihaela Dana
Published: November 2023

The pathophysiology of intracranial aneurysms (IA) has been proven to be closely linked to hemodynamic stress and inflammatory pathways, most notably the NF-kB pathway. Therefore, it is a potential target for therapeutic intervention. In the present review, we investigated alterations in the vascular smooth muscle cells (VSMCs), extracellular matrix, and endothelial cells by the mediators implicated in the NF-kB pathway that lead to the formation, growth, and rupture of IAs. We also present an overview of the NF-kB pathway, focusing on stimuli and transcriptional targets specific to IAs, as well as a summary of the current strategies for inhibiting NF-kB activation in IAs. Our report adds to previously reported data and future research directions for treating IAs using compounds that can suppress inflammation in the vascular wall.
The Association between Kidney Function Biomarkers and Delayed Memory Impairments among Older Adults in the European North of Russia
Authors: Poskotinova Liliya , Kontsevaya Anna , Kudryavtsev Alexander V.
Published: November 2023

The prevention of memory decline requires better knowledge of biological markers. We studied the associations between kidney function biomarkers and memory decline (assessed with the Mini-Mental State Examination—MMSE) in elderly individuals without dementia (MMSE 24–30, age 60–74 years, n = 643, Arkhangelsk, Russia). Participants were divided by sex and into three groups according to the delayed memory performance: recall of 0–1, 2, and 3 out of 3 words. The median of serum creatinine was 82 μmol/L in men who recalled 2 words and both medians in those recalling 3 and 0–1 words were 87 μmol/L. The 90th percentile for creatinine in men recalling 0–1 words (115.0 μmol/L) exceeded the upper limit of the normal range (110.5 μmol/L), while those who recalled 3 and 2 words had 90th percentiles within the normal range (109 and 101 μmol/L, respectively). Glomerular filtration rates were normal (≥60 mL/min/1.73 m2) with a median of 92.0 mL/min/1.73 m2 in men who recalled 2 words, 84.4 and 84.9 mL/min/1.73 m2 in men who recalled 3 and 0–1 words, respectively. None of these associations were observed in women. A reduced serum creatinine in older non-demented men may indicate the initial stages of memory decline, while the increased creatinine may reflect further stages of memory impairment.