bluuebunny/crossref_metadata_2025 huggingface dataset:
Diaphragmatic Activity and Respiratory Function Following C3 or C6 Unilateral Spinal Cord Contusion in Mice
Authors: Bajjig Afaf , Michel-Flutot Pauline , Migevent Tiffany , Cayetanot Florence , Bodineau Laurence , Vinit Stéphane , Vivodtzev Isabelle
Published: April 2022

The majority of spinal cord injuries (SCIs) are cervical (cSCI), leading to a marked reduction in respiratory capacity. We aimed to investigate the effect of hemicontusion models of cSCI on both diaphragm activity and respiratory function to serve as preclinical models of cervical SCI. Since phrenic motoneuron pools are located at the C3–C5 spinal level, we investigated two models of preclinical cSCI mimicking human forms of injury, namely, one above (C3 hemicontusion—C3HC) and one below phrenic motoneuron pools (C6HC) in wild-type swiss OF-1 mice, and we compared their effects on respiratory function using whole-body plethysmography and on diaphragm activity using electromyography (EMG). At 7 days post-surgery, both C3HC and C6HC damaged spinal cord integrity above the lesion level, suggesting that C6HC potentially alters C5 motoneurons. Although both models led to decreased diaphragmatic EMG activity in the injured hemidiaphragm compared to the intact one (−46% and −26% in C3HC and C6HC, respectively, both p = 0.02), only C3HC led to a significant reduction in tidal volume and minute ventilation compared to sham surgery (−25% and −20% vs. baseline). Moreover, changes in EMG amplitude between respiratory bursts were observed post-C3HC, reflecting a change in phrenic motoneuronal excitability. Hence, C3HC and C6HC models induced alteration in respiratory function proportionally to injury level, and the C3HC model is a more appropriate model for interventional studies aiming to restore respiratory function in cSCI.
Photodynamic Therapy in Combination with Doxorubicin Is Superior to Monotherapy for the Treatment of Lung Cancer
Authors: Cacaccio Joseph C. , Durrani Farukh A. , Missert Joseph R. , Pandey Ravindra K.
Published: April 2022

We have previously shown that a radioactive (123I)-analog of methyl 3-(1′-(iodobexyloxy) ethyl-3-devinylpyropheophorbide-a (PET-ONCO), derived from chlorophyll-a can be used for positron emission tomography (PET) imaging of a variety of tumors, including those where 18F-FDG shows limitations. In this study, the photodynamic therapy (PDT) efficacy of the corresponding non-radioactive photosensitizer (PS) was investigated in a variety of tumor types (NSCLC, SCC, adenocarcinoma) derived from lung cancer patients in mice tumor models. The in vitro and in vivo efficacy was also investigated in combination with doxorubicin, and a significantly enhanced long-term tumor response was observed. The toxicity and toxicokinetic profile of the iodinated PS was also evaluated in male and female Sprague-Dawley rats and Beagle dog at variable doses (single intravenous injections) to assess reversibility or latency of any effects over a 28-day dose free period. The no-observed-adverse-effect (NOAEL) of the PS was considered to be 6.5 mg/kg for male and female rats, and for dogs, 3.45 mg/kg, the highest dose levels evaluated, respectively. The corresponding plasma Cmax and AYClast for male and female rats were 214,000 and 229,000 ng/mL and 3,680,000 and 3,810,000 h * ng/mL, respectively. For male and female dogs, the corresponding plasma Cmax and AYClast were 76,000 and 92,400 ng/mL and 976,000 and 1,200,000 h * ng/mL, respectively.
Gut Microbiota Ecosystem Governance of Host Inflammation, Mitochondrial Respiration and Skeletal Homeostasis
Authors: Lian Wei-Shiung , Wang Feng-Sheng , Chen Yu-Shan , Tsai Ming-Hsien , Chao How-Ran , Jahr Holger , Wu Re-Wen , Ko Jih-Yang
Published: April 2022

Osteoporosis and osteoarthritis account for the leading causes of musculoskeletal dysfunction in older adults. Senescent chondrocyte overburden, inflammation, oxidative stress, subcellular organelle dysfunction, and genomic instability are prominent features of these age-mediated skeletal diseases. Age-related intestinal disorders and gut dysbiosis contribute to host tissue inflammation and oxidative stress by affecting host immune responses and cell metabolism. Dysregulation of gut microflora correlates with development of osteoarthritis and osteoporosis in humans and rodents. Intestinal microorganisms produce metabolites, including short-chain fatty acids, bile acids, trimethylamine N-oxide, and liposaccharides, affecting mitochondrial function, metabolism, biogenesis, autophagy, and redox reactions in chondrocytes and bone cells to regulate joint and bone tissue homeostasis. Modulating the abundance of Lactobacillus and Bifidobacterium, or the ratio of Firmicutes and Bacteroidetes, in the gut microenvironment by probiotics or fecal microbiota transplantation is advantageous to suppress age-induced chronic inflammation and oxidative damage in musculoskeletal tissue. Supplementation with gut microbiota-derived metabolites potentially slows down development of osteoarthritis and osteoporosis. This review provides latest molecular and cellular insights into the biological significance of gut microorganisms and primary and secondary metabolites important to cartilage and bone integrity. It further highlights treatment options with probiotics or metabolites for modulating the progression of these two common skeletal disorders.
Development and Validation of a Raman Spectroscopic Classification Model for Cervical Intraepithelial Neoplasia (CIN)
Authors: Traynor Damien , Duraipandian Shiyamala , Bhatia Ramya , Cuschieri Kate , Tewari Prerna , Kearney Padraig , D’Arcy Tom , O’Leary John J. , Martin Cara M. , Lyng Fiona M.
Published: April 2022

The mortality associated with cervical cancer can be reduced if detected at the precancer stage, but current methods are limited in terms of subjectivity, cost and time. Optical spectroscopic methods such as Raman spectroscopy can provide a rapid, label-free and nondestructive measurement of the biochemical fingerprint of a cell, tissue or biofluid. Previous studies have shown the potential of Raman spectroscopy for cervical cancer diagnosis, but most were pilot studies with small sample sizes. The aim of this study is to show the clinical utility of Raman spectroscopy for identifying cervical precancer in a large sample set with validation in an independent test set. Liquid-based cervical cytology samples (n = 662) (326 negative, 200 cervical intraepithelial neoplasia (CIN)1 and 136 CIN2+) were obtained as a training set. Raman spectra were recorded from single-cell nuclei and subjected to a partial least squares discriminant analysis (PLSDA). In addition, the PLSDA classification model was validated using a blinded independent test set (n = 69). A classification accuracy of 91.3% was achieved with only six of the blinded samples misclassified. This study showed the potential clinical utility of Raman spectroscopy with a good classification of negative, CIN1 and CIN2+ achieved in an independent test set.
Quantifying Tumor Heterogeneity via MRI Habitats to Characterize Microenvironmental Alterations in HER2+ Breast Cancer
Authors: Kazerouni Anum S. , Hormuth David A. , Davis Tessa , Bloom Meghan J. , Mounho Sarah , Rahman Gibraan , Virostko John , Yankeelov Thomas E. , Sorace Anna G.
Published: April 2022

This study identifies physiological habitats using quantitative magnetic resonance imaging (MRI) to elucidate intertumoral differences and characterize microenvironmental response to targeted and cytotoxic therapy. BT-474 human epidermal growth factor receptor 2 (HER2+) breast tumors were imaged before and during treatment (trastuzumab, paclitaxel) with diffusion-weighted MRI and dynamic contrast-enhanced MRI to measure tumor cellularity and vascularity, respectively. Tumors were stained for anti-CD31, anti-ɑSMA, anti-CD45, anti-F4/80, anti-pimonidazole, and H&E. MRI data was clustered to identify and label each habitat in terms of vascularity and cellularity. Pre-treatment habitat composition was used stratify tumors into two “tumor imaging phenotypes” (Type 1, Type 2). Type 1 tumors showed significantly higher percent tumor volume of the high-vascularity high-cellularity (HV-HC) habitat compared to Type 2 tumors, and significantly lower volume of low-vascularity high-cellularity (LV-HC) and low-vascularity low-cellularity (LV-LC) habitats. Tumor phenotypes showed significant differences in treatment response, in both changes in tumor volume and physiological composition. Significant positive correlations were found between histological stains and tumor habitats. These findings suggest that the differential baseline imaging phenotypes can predict response to therapy. Specifically, the Type 1 phenotype indicates increased sensitivity to targeted or cytotoxic therapy compared to Type 2 tumors.
Conversion of Hyperpolarized [1-13C]Pyruvate in Breast Cancer Cells Depends on Their Malignancy, Metabolic Program and Nutrient Microenvironment
Authors: Grashei Martin , Biechl Philipp , Schilling Franz , Otto Angela M.
Published: April 2022

Hyperpolarized magnetic resonance spectroscopy (MRS) is a technology for characterizing tumors in vivo based on their metabolic activities. The conversion rates (kpl) of hyperpolarized [1-13C]pyruvate to [1-13C]lactate depend on monocarboxylate transporters (MCT) and lactate dehydrogenase (LDH); these are also indicators of tumor malignancy. An unresolved issue is how glucose and glutamine availability in the tumor microenvironment affects metabolic characteristics of the cancer and how this relates to kpl-values. Two breast cancer cells of different malignancy (MCF-7, MDA-MB-231) were cultured in media containing defined combinations of low glucose (1 mM; 2.5 mM) and glutamine (0.1 mM; 1 mM) and analyzed for pyruvate uptake, intracellular metabolite levels, LDH and pyruvate kinase activities, and 13C6-glucose-derived metabolomics. The results show variability of kpl with the different glucose/glutamine conditions, congruent with glycolytic activity, but not with LDH activity or the Warburg effect; this suggests metabolic compartmentation. Remarkably, kpl-values were almost two-fold higher in MCF-7 than in the more malignant MDA-MB-231 cells, the latter showing a higher flux of 13C-glucose-derived pyruvate to the TCA-cycle metabolites 13C2-citrate and 13C3-malate, i.e., pyruvate decarboxylation and carboxylation, respectively. Thus, MRS with hyperpolarized [1-13C-pyruvate] is sensitive to both the metabolic program and the nutritional state of cancer cells.
HuR Plays a Role in Double-Strand Break Repair in Pancreatic Cancer Cells and Regulates Functional BRCA1-Associated-Ring-Domain-1(BARD1) Isoforms
Authors: Jain Aditi , McCoy Matthew , Coats Carolyn , Brown Samantha Z. , Addya Sankar , Pelz Carl , Sears Rosalie C. , Yeo Charles J. , Brody Jonathan R.
Published: April 2022

Human Antigen R (HuR/ELAVL1) is known to regulate stability of mRNAs involved in pancreatic ductal adenocarcinoma (PDAC) cell survival. Although several HuR targets are established, it is likely that many remain currently unknown. Here, we identified BARD1 mRNA as a novel target of HuR. Silencing HuR caused a >70% decrease in homologous recombination repair (HRR) efficiency as measured by the double-strand break repair (pDR-GFP reporter) assay. HuR-bound mRNAs extracted from RNP-immunoprecipitation and probed on a microarray, revealed a subset of HRR genes as putative HuR targets, including the BRCA1-Associated-Ring-Domain-1 (BARD1) (p < 0.005). BARD1 genetic alterations are infrequent in PDAC, and its context-dependent upregulation is poorly understood. Genetic silencing (siRNA and CRISPR knock-out) and pharmacological targeting of HuR inhibited both full length (FL) BARD1 and its functional isoforms (α, δ, Φ). Silencing BARD1 sensitized cells to olaparib and oxaliplatin; caused G2-M cell cycle arrest; and increased DNA-damage while decreasing HRR efficiency in cells. Exogenous overexpression of BARD1 in HuR-deficient cells partially rescued the HRR dysfunction, independent of an HuR pro-oncogenic function. Collectively, our findings demonstrate for the first time that BARD1 is a bona fide HuR target, which serves as an important regulatory point of the transient DNA-repair response in PDAC cells.
Impact of Lipid Metabolism on Antitumor Immune Response
Authors: Mabrouk Nesrine , Lecoeur Baptiste , Bettaieb Ali , Paul Catherine , Végran Frédérique
Published: April 2022

Over the past decade, metabolic reprogramming has been defined as a hallmark of cancer. More recently, a large number of studies have demonstrated that metabolic reprogramming can modulate the differentiation and functions of immune cells, and thus modify the antitumor response. Increasing evidence suggests that modified energy metabolism could be responsible for the failure of antitumor immunity. Indeed, tumor-infiltrating immune cells play a key role in cancer, and metabolic switching in these cells has been shown to help determine their phenotype: tumor suppressive or immune suppressive. Recent studies in the field of immunometabolism focus on metabolic reprogramming in the tumor microenvironment (TME) by targeting innate and adaptive immune cells and their associated anti- or protumor phenotypes. In this review, we discuss the lipid metabolism of immune cells in the TME as well as the effects of lipids; finally, we expose the link between therapies and lipid metabolism.
The EGFR Signaling Modulates in Mesenchymal Stem Cells the Expression of miRNAs Involved in the Interaction with Breast Cancer Cells
Authors: Gallo Marianna , Carotenuto Marianeve , Frezzetti Daniela , Camerlingo Rosa , Roma Cristin , Bergantino Francesca , Normanno Nicola , De Luca Antonella
Published: April 2022

We previously demonstrated that the epidermal growth factor receptor (EGFR) modulates in mesenchymal stem cells (MSCs) the expression of a number of genes coding for secreted proteins that promote breast cancer progression. However, the role of the EGFR in modulating in MSCs the expression of miRNAs potentially involved in the progression of breast cancer remains largely unexplored. Following small RNA-sequencing, we identified 36 miRNAs differentially expressed between MSCs untreated or treated with the EGFR ligand transforming growth factor α (TGFα), with a fold change (FC) < 0.56 or FC ≥ 1.90 (CI, 95%). KEGG analysis revealed a significant enrichment in signaling pathways involved in cancer development and progression. EGFR activation in MSCs downregulated the expression of different miRNAs, including miR-23c. EGFR signaling also reduced the secretion of miR-23c in conditioned medium from MSCs. Functional assays demonstrated that miR-23c acts as tumor suppressor in basal/claudin-low MDA-MB-231 and MDA-MB-468 cells, through the repression of IL-6R. MiR-23c downregulation promoted cell proliferation, migration and invasion of these breast cancer cell lines. Collectively, our data suggested that the EGFR signaling regulates in MSCs the expression of miRNAs that might be involved in breast cancer progression, providing novel information on the mechanisms that regulate the MSC-tumor cell cross-talk.
H2 Photoproduction Efficiency: Implications of the Reaction Mechanism as a Function of the Methanol/Water Mixture
Authors: Barba-Nieto Irene , Colón Gerardo , Kubacka Anna , Fernández-García Marcos
Published: April 2022

The influence of the reaction pathway of the sacrificial molecule oxidation to generate hydrogen is here investigated for lean and rich methanol reaction mixtures. Pt-TiO2 powders promoted or not with tin sulfide were used as catalysts. With the help of in situ infrared experiments under reaction conditions, methanol evolution was shown to take place by hole-related oxidation steps, with alkoxy and carbon-centered species as key radical species. The study analyzed quantitatively the fate and chemical use of the photons absorbed by the solids with the help of the quantum efficiency and the useful fraction of photons observables. Within this framework, the role of the sulfide component to promote photoactivity is interpreted, braiding chemical and photonic information.
Morphological and Immunopathological Aspects of Lingual Tissues in COVID-19
Authors: Henin Dolaji , Pellegrini Gaia , Carmagnola Daniela , Lanza Attisano Giuseppe Carlo , Lopez Gianluca , Ferrero Stefano , Amendola Antonella , De Angelis Danilo , Tanzi Elisabetta , Dellavia Claudia
Published: April 2022

COVID-19, a recently emerged disease caused by SARS-CoV-2 infection, can present with different degrees of severity and a large variety of signs and symptoms. The oral manifestations of COVID-19 often involve the tongue, with loss of taste being one of the most common symptoms of the disease. This study aimed to detect SARS-CoV-2 RNA and assess possible morphological and immunopathological alterations in the lingual tissue of patients who died with a history of SARS-CoV-2 infection. Sixteen cadavers from 8 SARS-CoV-2 positive (COVID-19+) and 8 negative (COVID-19−) subjects provided 16 tongues, that were biopsied. Samples underwent molecular analysis through Real-Time RT-PCR for the detection of SARS-CoV-2 RNA. Lingual papillae were harvested and processed for histological analysis and for immunohistochemical evaluation for ACE2, IFN-γ and factor VIII. Real-Time RT-PCR revealed the presence of SARS-CoV-2 RNA in filiform, foliate, and circumvallate papillae in 6 out of 8 COVID-19+ subjects while all COVID-19− samples resulted negative. Histology showed a severe inflammation of COVID-19+ papillae with destruction of the taste buds. ACE2 and IFN-γ resulted downregulated in COVID-19+ and no differences were evidenced for factor VIII between the two groups. The virus was detectable in most COVID-19+ tongues. An inflammatory damage to the lingual papillae, putatively mediated by ACE2 and IFN-γ in tongues from COVID-19+ cadavers, was observed. Further investigations are needed to confirm these findings and deepen the association between taste disorders and inflammation in SARS-CoV-2 infection.
Enhanced Photocatalytic Oxidation of RhB and MB Using Plasmonic Performance of Ag Deposited on Bi2WO6
Authors: Khanam Shomaila , Rout Sanjeeb Kumar
Published: April 2022

Visible-light-driven heterostructure Ag/Bi2WO6 nanocomposites were prepared using a hydrothermal method followed by the photodeposition of Ag on Bi2WO6. A photocatalyst with a different molar ratio of Ag to Bi2WO6 (1:1, 1:2 and 2:1) was prepared. The catalytic performance of Ag/Bi2WO6 towards the photocatalytic oxidation of rhodamine B (RhB) and methylene blue (MB) was explored. Interestingly, the Ag/Bi2WO6 (1:2) catalyst exhibited superior performance; it oxidized 83% of RhB to Rh-110 and degraded 68% of MB in 90 min. This might be due to the optimum amount of Ag nanoparticles, which supported the rapid generation and transfer of separated charges from Bi2WO6 to Ag through the Schottky barrier. An excess of Ag on Bi2WO6 (1:1 and 2:1) blocked the active sites of the reaction and did not produce the desired result. The introduction of Ag on Bi2WO6 improved the electrical conductivity of the composite and lowered the recombination rate of charge carriers. Our work provides a cost-effective route for constructing high-performance catalysts for the degradation of toxic dyes.
Design, Elaboration, and Characterization of an Immunosensor for the Detection of a Fungal Toxin in Foodstuff Analyses
Authors: Ben Abdallah Zeineb , Sghaier Halim , Gammoudi Ibtissem , Moroté Fabien , Cassagnère Sébastien , Romo Lena , Béven Laure , Grauby-Heywang Christine , Cohen-Bouhacina Touria
Published: April 2022

This work describes the complete elaboration of an immunosensor for the detection of the fungal B1 aflatoxin (AFB1). In a first step, a system made of three screen-printed electrodes (SPEs) was manufactured using gold, silver/silver chloride, and carbon pastes. Raman spectroscopy showed that the thermal treatment applied to the electrodes enabled a strong decrease in the amount of undesirable organic molecules for each paste. Atomic Force Microscopy was also used to reveal the morphology of the electrode surfaces. In a second step, an autonomous and cheap electronic system was designed for the control of the sensor and electrochemical measurements, showing current variations significantly higher than those observed with a commercial system. In a last step, the gold working electrode of this system was functionalized by a simple self-assembly method, optimized in a previous work, with a molecular architecture including an antibody recognizing specifically AFB1. The complete device was finally realized by combining the SPEs and the electronic platform. The resulting setup was able to detect AFB1 toxin in a buffer with an LOD of about 50 fg/mL. It was then applied to the detection of AFB1 in rice milk, a more realistic medium comparable with those met in an agrifood context. The electrochemical detection of AFB1 was possible in a range of concentration between 0.5 pg/mL and 2.5 pg/mL, with the sensor behaving linearly in this range.
The Evaluation of FGFR1, FGFR2 and FOXO1 in Orofacial Cleft Tissue
Authors: Goida Jana , Pilmane Mara
Published: April 2022

Although cleft lip with or without cleft palate (CL/P) is one of the most common congenital anomalies worldwide, the morphopathogenesis of non-syndromic orofacial clefts is still unclear. Many candidate genes have been proposed to play a causal role; however, only a few have been confirmed, leaving many still to be assessed. Taking into account the significance of FGFR1, FGFR2 and FOXO1 in embryogenesis, the aim of this work was to detect and compare the three candidate genes in cleft-affected lip and palatine tissue. Ten soft tissue samples were taken during cheiloplasty and veloplasty. The signals of the candidate genes were visualized using chromogenic in situ hybridization and analyzed using a semi-quantitative method. No statistically important difference in the distribution of FGFR1, FGFR2 and FOXO1 between neither the patients’ lip and vomer mucosa nor the control group was observed. Statistically significant very strong and strong correlations were found between genes in the lip and palatine tissue. The expression of FGFR1, FGFR2 and FOXO1 in cleft-affected lip and palatine tissue seems to be highly individual. Numerous intercorrelations between the genes do not exclude their role in the possible complex morphopathogenesis of orofacial clefts.
ERβ Isoforms Have Differential Clinical Significance in Breast Cancer Subtypes and Subgroups
Authors: Choi Young , Kim Hadong , Pollack Simcha
Published: April 2022

ERβ, an ER subtype first identified in 1996, is highly expressed in different types of BCa including ERα-negative BCa and TNBC. Many studies on ERβ expression investigated mostly on ERβ1 protein expression in ERα-positive and ERα-negative BCa combined. The results are conflicting. This may be due to the complexity of ERβ isoforms, subject heterogeneity, and various study designs targeting different ERβ isoforms and either ERβ protein or mRNA expression, as well as to the lack of a standardized testing protocol. Herein, we simultaneously investigated both mRNA and protein expression of ERβ isoforms 1, 2, and 5 in different BCa subtypes and clinical characteristics. Patient samples (138) and breast cancer cell lines (BCC) reflecting different types of BCa were tested for ERα and ERβ mRNA expression using quantitative real-time PCR, as well as for protein expression of ERα, ERβ1, ERβ2, and ERβ5 isoforms, PR, HER2/neu, Ki-67, CK 5/6, and p53 using immunohistochemistry. Associations of ERβ isoform expression with clinical characteristics and overall survival (OS) were analyzed. ERβ1, 2, and 5 isoforms are differentially expressed in different BCa subtypes including ERα-negative and TNBC. Each ERβ isoform seemingly plays a distinct role and is associated with clinical tumor characteristics and patient outcomes. ERβ isoform expression is significantly associated with >15% Ki-67 positivity and poor prognostic markers, and it predicts poorer OS, mostly in the subgroups. High ERβ2 and 5 isoform expression in ERα-negative BCa and TNBC is predictive of poor OS. Further investigation of ERβ isoforms in a larger cohort of BCa subgroups is needed to evaluate the role of ERβ for the potential usefulness of ERβ as a prognostic and predictive marker and for therapeutic use. The inconsistent outcomes of ERβ isoform mRNA or protein expression in many studies suggest that the standardization of ERβ testing would facilitate the use of ERβ in a clinical setting.
Climate Changes in Southeastern Poland and Food Security
Authors: Sawicka Barbara , Barbaś Piotr , Pszczółkowski Piotr , Skiba Dominika , Yeganehpoor Farhood , Krochmal-Marczak Barbara
Published: April 2022

The conducted research is of particular importance for the country’s food security in the context of climate change in Southeastern Poland. The aim of the research was to determine the influence of climate on the variability of the appearance and the rate of spread of potato blights as the main factor limiting the potato yield in the conditions of Central and Eastern Europe. Combined statistical and simulation modeling methods were used. A mixed effect model was used to detect the effects of temperature, humidity, rainfall and wind speed on potato yield, and partial regression analysis models were used. The natural, agricultural and economic conditions in terms of suitability for potato cultivation were assessed, and factors influencing the fluctuation of the cultivated acreage, yield and harvesting of potatoes were identified. The forecast was based on empirical data from 2000 to 2019. It has been proven that potato cultivation in Southeastern Poland is more vulnerable to climate change than in the rest of the country. The results obtained from analyzing multi-annual results can help policymakers to develop strategies to increase the stability of future potato production and the safety of the crop. This will enable the better use of generated data and methodological approaches to analyze the role of climate, both on a regional and global scale.
Assessing the Use of Aloe vera Gel Alone and in Combination with Lemongrass Essential Oil as a Coating Material for Strawberry Fruits: HPLC and EDX Analyses
Authors: Hassan Hanaa S. , EL-Hefny Mervat , Ghoneim Ibrahim M. , El-Lahot Mina S. R. Abd , Akrami Mohammad , Al-Huqail Asma A. , Ali Hayssam M. , Abd-Elkader Doaa Y.
Published: April 2022

Strawberry is a non-climacteric fruit but exhibits a limited postharvest life due to rapid softening and decay. A strawberry coating that is natural and safe for human consumption can be used to improve the appearance and safeguard the fruits. In this study, 20% and 40% Aloe vera gel alone or in combination with 1% lemongrass essential oil (EO) was used as an edible coating for strawberries. After application of all the treatments, the strawberry fruits were stored at a temperature of 5 ± 1 °C at a relative humidity (RH) of 90%–95% for up to 16 days and all the parameters were analyzed and compared to control (uncoated fruits). The results show that A. vera gel alone or with lemongrass EO reduced the deterioration and increased the shelf life of the fruit. Treatment with A. vera gel and lemongrass EO decreased acidity and total anthocyanins and maintained fruit firmness. Treatment with A. vera gel 40% + lemongrass EO 1% led to the lowest weight loss, retained firmness and acidity, but increased the total soluble solids and total anthocyanins compared to uncoated fruits during storage of up to 16 days. The phenolic compounds of A. vera gel were analyzed by HPLC, and the most abundant compounds were found to be caffeic (30.77 mg/mL), coumaric (22.4 mg/mL), syringic (15.12 mg/mL), sinapic (14.05 mg/mL), ferulic (8.22 mg/mL), and cinnamic acids (7.14 mg/mL). Lemongrass EO was analyzed by GC–MS, and the most abundant compounds were identified as α-citral (neral) (40.10%) ꞵ-citral (geranial) (30.71%), γ-dodecalactone (10.24%), isoneral (6.67%), neryl acetal (5.64%), and linalool (1.77%). When the fruits were treated with 20% or 40% A. vera gel along with 1% lemongrass, their total phenolic content was maintained during the storage period (from 4 to 8 days). The antioxidant activity was relatively stable during the 8 days of cold storage of the fruits coated with A. vera gel combined with lemongrass EO because the activity of both 20% and 40% gel was greater than that for the other treatments after 12 days of storage in both experiments. Moreover, all the treatments resulted in lower numbers of total microbes at the end of the storage period compared with the control treatment. This study indicates that the use of Aloe vera gel with lemongrass EO as an edible coating considerably enhances the productivity of strawberry fruits and the treatment could be used on a commercial scale.
Tribochemistry of Transfer Layer Evolution during Friction in HiPIMS W-C and W-C:H Coatings in Humid Oxidizing and Dry Inert Atmospheres
Authors: Lofaj František , Tanaka Hiroyoshi , Bureš Radovan , Kabátová Margita , Sawae Yoshinori
Published: April 2022

The experimental and theoretical investigations of transfer layers in the dry sliding contacts between steel ball and HiPIMS W-C and W-C:H coatings were performed in humid air, dry nitrogen, hydrogen and vacuum on a series of coatings with different contents of carbon and hydrogen in the matrix. Transfer layers formed on the ball in all friction tests, but their composition varied depending on the environment. In humid air, the mechano(tribo)chemical reactions necessary for the obtained phases involved oxidation of WC and Fe, water vapor decomposition and hydrogenation of carbon. Modeling indicated that humidity enhanced oxidation and carbon hydrogenation. In nitrogen, WC decomposition generating carbon was dominant, whereas, in hydrogen, it was carbon hydrogenation. In vacuum, WC decomposition producing W was found to be responsible for high coefficients of friction (COFs). COFs approaching superlubricity were obtained in the H2 atmosphere in the coatings with sufficiently high matrix C:H content. COFs seem to be controlled by the ratio of hydrogenated carbon and oxide phases in transfer layer, which depends on the reactions possible in the surrounding atmosphere.
Characterization of Thermal, Ionic Conductivity and Electrochemical Properties of Some p-Tosylate Anions-Based Protic Ionic Compounds
Authors: Anis Arfat , Alam Manawwer , Alhamidi Abdullah , Alam Mohammad Asif , Gupta Ravindra Kumar , Tariq Mohammad , Shaikh Hamid , Poulose Anesh Manjaly , Al-Zahrani Saeed M.
Published: April 2022

In the present work, six protic ionic liquid (PIL) compounds based on p-toluene sulfonic acid [PTSA] anion along with different cations viz. tetraethylenepentammonium [TEPA], triethylammonium [TEA], pyridinium [Py], N-methylpiperidinium [Pip], 1-methylimidazolium [Im], and N-methylpyrrolidinium [Pyrr] were synthesized using the standard neutralization reaction method. The structural characterization of these compounds was achieved using FTIR, 1H and 13C NMR spectroscopies. Thermal behavior was studied using differential scanning calorimetry to determine the melting point (Tm) and crystallization (Tc) temperatures. Thermogravimetric analysis was carried out to determine the thermal stability and degradation temperatures (Tdec) and to ascertain the hygroscopic or hydrophobic nature of the synthesized compounds. Structural effects on the outcome of various properties were witnessed and discussed in detail. Electrochemical impedance spectroscopy was utilized to study the electrical transport properties of the PILs at different temperatures. Cyclic voltammetry was performed to analyze the electrochemical stability of these PILs. Low values of activation energy indicating easy proton transportation along with good electrochemical stability make the PILs a potential candidate for use in the preparation of polymer electrolytes membranes for fuel cell applications.
Effect of Non-Covalent Interactions on the 2,4- and 3,5-Dinitrobenzoate Eu-Cd Complex Structures
Authors: Shmelev Maxim A. , Chistyakov Aleksandr S. , Razgonyaeva Galina A. , Kovalev Vladimir V. , Voronina Julia K. , Dolgushin Fedor M. , Gogoleva Natalia V. , Kiskin Mikhail A. , Sidorov Alexey A. , Eremenko Igor L.
Published: April 2022

Heterometallic {Eu2Cd2} complexes [Eu2(NO3)2Cd2(Phen)2(2,4-Nbz)8]n·2nMeCN (I) and [Eu2(MeCN)2Cd2(Phen)2(3,5-Nbz)10] (II) with the 2,4-dinitrobenzoate (2,4-Nbz) and 3,5-dinitrobenzoate (3,5-Nbz) anions and 1,10-phenanthroline were synthesized. The compounds obtained were characterized by X-ray single-crystal analysis, powder X-ray diffraction analysis, IR spectroscopy, and elemental analysis. Moreover, the thermal stability of the complexes was also studied. Analysis of the crystal packing showed that where 1,10-phenanthroline is combined with various isomers of dinitrobenzoate anions, different arrangements of non-covalent interactions are observed in the complex structures. In the case of the compound with the 2,4-dinitrobenzoate anion, these interactions lead to a significant distortion of the metal core geometry and formation of a polymeric structure, while the complex with the 3,5-dinitrobenzoate anion has a structure that is typical of similar systems. The absence of europium metal-centered luminescence at 270 nm wavelength was shown. For all the reported compounds, a thermal stability study was carried out that showed that the compounds decomposed with a significant thermal effect.
Advanced Catalysts for the Water Gas Shift Reaction
Authors: Baraj Erlisa , Ciahotný Karel , Hlinčík Tomáš
Published: April 2022

The WGS reaction is an exothermic reaction between carbon monoxide and steam to form carbon dioxide and hydrogen. This reaction, which has been used industrially for more than 100 years, has recently received a great deal of attention from researchers as one of the ways to produce environmentally acceptable hydrogen from fossil fuels in large quantities. For the application of this reaction on an industrial scale, the key is choosing the optimal catalysts that can ensure high CO conversion and have a long lifetime under industrial conditions. Therefore, new types of catalysts are being developed that meet these requirements better than the Fe- and Cu-based catalysts commonly used in the past. The WGSR on a commercial nickel-based catalyst and a laboratory-prepared copper- and cobalt-based catalyst was tested in a laboratory apparatus set up at the University of Chemistry and Technology Prague. The best performance of the laboratory-prepared catalyst was observed for the catalyst with a Cu content of 14.8 wt% and activated in a hydrogen atmosphere. The laboratory-prepared Co-based catalyst showed good WGSR activity in the temperature range of 200–450 °C, although this was always inferior to that of the Cu-based catalyst. When subjected to the feed gas containing 0.4 mole% H2S, the Co-based catalyst showed good resistance to sulphur poisoning. Therefore, Co-based catalysts can be considered good sulphur-tolerant intermediate temperature WGSR catalysts.
Population Genetic Structure and Diversity of Metaphire remanens (Oligochaeta: Megascolecidae) Based on Mitochondrial DNA Analysis, with a Note on a New Species of Metaphire remanens sp. nov.
Authors: Jin Qing , Jiang Jibao , Li Jiali , Qiu Jiangping
Published: April 2022

Metaphire remanens sp. nov. is widely distributed throughout Hunan Province, China. We sequenced the mitochondrial DNA to investigate its population genetic structure and genetic diversity, including cytochrome c oxidase subunit I, cytochrome c oxidase subunit II, 12S ribosomal (r)RNA, 16S rRNA, and nicotinamide adenine dinucleotide dehydrogenase subunit 1, derived from 39 individuals from seven geographic locations in Hunan Province. The genetic diversity indices showed that populations of M. remanens have a strong genetic structure and obvious dispersal histories. M. remanens did not experience population expansion, except in Xiangtan City. This may be because of its evolution toward parthenogenesis. The divergence time estimates indicated that M. remanens originated at 19.2055 Ma and then generated two main lineages at 1.7334 Ma (Quaternary glaciation). These results indicate that glaciation, geographic isolation, and dispersal ability are significant factors that influence the differentiation and dispersal of M. remanens. In this study, we describe Metaphire remanens sp. nov. in morphology.
Silencer Design for the Control of Low Frequency Noise in Ventilation Ducts
Authors: Piana Edoardo Alessio , Carlsson Ulf Erik , Lezzi Adriano Maria , Paderno Diego , Boij Susann
Published: April 2022

The control of noise propagating along ventilation system ducts has always been an important issue in the building and vehicle sectors. This problem is generally tackled by selecting noise-reducing components with a suitable transmission loss, possibly verifying their effectiveness at a later time. The aim of this article is to characterize the nature of the problem and propose a design approach focusing directly on the perceived effect, that is, on the sound pressure level downstream of the outlet. Because the nature of the noise emission depends on various generation mechanisms, different methods can be applied. Usually, it is more difficult to realize good attenuations at low frequencies because of the limits of sound absorbing materials in such frequency range. For this reason, the ability of reactive components to attenuate the noise below the cut-on frequency will be investigated. This goal is reached by applying the transfer matrix approach to a duct system, with the implementation of the transfer matrices of each single element, and then assembling a system capable of acoustically describing the source and the duct structure. The coupling between the duct system with source and receiver impedances allows one to predict the sound pressure level at a given distance from the outlet. The proposed methodology is implemented in a user-friendly calculation tool with possible academic and professional application. Predictive capability, usability, and intuitiveness of the proposed design procedure are validated against experimental results by real potential users, who express positive feedback.
COVID-19 Pandemic Effects on Cervical Cancer Diagnosis and Management: A Population-Based Study in Romania
Authors: Popescu Alin , Craina Marius , Pantea Stelian , Pirvu Catalin , Chiriac Veronica Daniela , Marincu Iosif , Bratosin Felix , Bogdan Iulia , Hosin Samer , Citu Cosmin , Bernad Elena , Neamtu Radu , Dumitru Catalin , Mocanu Adelina Geanina , Avram Claudiu , Gluhovschi Adrian
Published: April 2022

The Pap test plays a significant role worldwide in the early diagnosis of and high curability rates for cervical cancer. However, the coronavirus disease 2019 (COVID-19) pandemic necessitated the use of multiple drastic measures to stop the spread of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, limiting women’s access to essential invasive and non-invasive investigations for cervical cancer diagnosis. Therefore, we aimed to determine the impact the COVID-19 pandemic had on cancer diagnosis and management in western Romania. A retrospective study design allowed us to compare the last 24 months of the pre-pandemic period with the first 24 months of the COVID-19 pandemic to determine the change in volume of cervical screening tests, the number of newly diagnosed cases and their severity, and the access to cancer care. A drastic 75.5% decrease in the volume of tests was observed in April 2020 during the first lockdown, after which the volume of cases decreased by up to 36.1% in December 2021. The total volume loss of tests during the first 24 months of the pandemic was 49.9%. The percentage of late-stage cervical cancers (III–IV) rose by 17%, while the number of newly diagnosed cancers in our outpatient clinic was significantly lower than the baseline, with a 45% drop. The access to cancer care was negatively influenced, with 9.2% more patients waiting longer to receive test results over four weeks, while taking longer to seek cancer care after diagnosis (6.4 months vs. 4.1 months pre-pandemic) and missing significantly more appointments. The COVID-19 pandemic had a significantly negative impact on cervical cancer diagnosis and management during the first 24 months compared with the same period before the pandemic. Although the numbers are now recovering, there is still a big gap, meaning that many cervical cancer cases were potentially missed. We recommend further interventions to reduce the gap between the pre-pandemic and pandemic period.
Improving Blood Pressure Control Using Digital Communication Methods in Serbia
Authors: Tasic Nebojsa , Tasic Danijela , Kovacevic Zorana , Filipovic Marko , Arsic Milan , Bozovic-Ogarevic Sladjana , Despotovic Biljana , Bojic Milovan , Maksimovic Zlatko , Zdravkovic Nebojsa , Mijailovic Sara , Zivkovic Vladimir , Nikolic Turnic Tamara , Jakovljevic Vladimir
Published: April 2022

Background: The purpose of this study was to compare home and office BP in the adjustment of antihypertensive treatment. Methods: This study was an open, prospective, noninterventional, multicenter clinical trial that occurred between July 2019 and February 2020, in 34 cities in the territory of the Republic of Serbia, which monitored 1581 participants for 6 months. Depending on the used blood pressure monitoring method used, all patients were divided into control (office BP monitoring) and experimental (home BP telemonitoring) groups. We collected anamnestic data and data about systolic blood pressure (SP), in mmHg, diastolic blood pressure (DP), in mmHg, and heart rate (HR), in beats/minute, from all patients. Results: SP values were significantly different at baseline, and at the second, third, and fourth visits between the two tested groups. Home and office BP decreased significantly (p < 0.000) during the 6-month follow-up. We observed a statistically significant influence of the presence of diabetes mellitus and dyslipidemia on the dynamics of differences between SP monitoring values. Conclusions: Our study suggests that novel technologies in BP monitoring can be excellent alternatives for BP assessment in hypertensive patients with other cardiovascular risk factors such as diabetes and dyslipidemia.
Lipid Profiles and Cardiovascular Risk in Patients with Oral Lichen Planus
Authors: Radic Tomislav , Cigic Livia , Glavina Ana , Hrboka Ana , Druzijanic Ana , Musa Leko Ivona , Biocina-Lukenda Dolores
Published: April 2022

Background: It has been reported that dyslipidemia prevalence and cardiovascular disease risk were increased in subjects with oral lichen planus diagnose. On the other hand, so far, there is no available data on the topic of cardiovascular risk (CVR) in subjects with oral lichen planus (OLP). The main aim of this study, due to lack of any other study covering this topic, was to investigate lipid profile and assess CVR in patients with OLP. Materials and Methods: To create a routine lipid profile, we collected triglyceride serum levels, total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol from 63 OLP patients and 63 healthy people representing control subjects. For every patient their individual cardiovascular risk was measured. Results: In comparison with the tested control subjects, patients with OLP had all parameters of the lipid profile elevated, with no differences of statistical importance. Furthermore, the experimental (OLP) and control groups shared similar mean values of the lipid profile parameters. Conclusions: The association of OLP with cardiovascular risk was not established and further studies with more subjects involved are required to validate this connection.
A Novel Approach to Immediate Implants: The CastleWall Surgical Technique
Authors: Castle Cameron
Published: April 2022

Objective: The purpose of this study was to investigate the volumetric stability around immediate implants, in which a 360-degree socket-shield was retained using the CastleWall Surgical Technique (CWST). Material and methods: This retrospective study examined the results of the CWST used for 25 consecutive patients, involving 31 immediate implants. Silicone impressions taken prior to extraction, and at a review appointment were converted to STL files and compared. The median follow-up time was 14.2 ± 5.5 months. Volumetric changes and gingival recession on both buccal and lingual sites were measured. Papillary height changes were also evaluated from available photographs taken before and after treatment. Patients in the study completed a Visual Analogue Scale (VAS) for evaluation of post-operative discomfort and overall satisfaction with this procedure. Results: All implants integrated successfully without complications. Mean loss of buccal and lingual tissue was 0.30 ± 0.32 mm and 0.17 ± 0.27 mm, respectively. Mean recession at the mid-buccal and mid-lingual gingival margin was 0.66 ± 0.64 mm and 0.87 ± 0.84 mm, respectively. Mean recession of the mesial and distal papilla was 0.26 ± 0.55 mm and 0.29 ± 0.52 mm, respectively. Patients reported 97.74 ± 5.60% satisfaction with this procedure using the Visual Analogue Scale (VAS), with minimal post-operative discomfort. Conclusions: The results of this study showed excellent soft tissue stability and aesthetics were achieved using the CWST, with minimal postoperative pain. The other main advantage of retaining a 360-degree socket-shield, is there is more available surface area to lock the implant to the shield to prevent shield migration over time.
Molar Incisor Hypomineralization: Awareness among Postdoctoral Dental Residents: A Cross-Sectional Study
Authors: Negrescu Jana , Kodra Laurenc , Ziada Hassan , Al-Talib Tanya , Abubakr Neamat Hassan
Published: April 2022

Background: Molar incisor hypomineralization (MIH) is the presentation of an enamel defect, where incisors and one (or more) molars are affected. Identifying MIH is significant in restoring its visual defect and avoiding pain or other consequences of this condition. The present cross-sectional study aimed to evaluate the awareness, ability, and confidence in identifying MIH among postgraduate residents in the state of Nevada. Methods: This cross-sectional study was conducted among postdoctoral dental residents at the School of Dental Medicine, University of Nevada, Las Vegas. This cross-sectional study used images of cases of MIH and a survey to collect the data. The survey included demographics, educational background, and basic knowledge of MIH. Results: The response rate to the invitation to participate was 91%. The confidence in identifying MIH was 100%, 50%, and 33.3% for pediatric, orthodontic, and general practice residency (GPR). A total of 70% were aware of this anomaly from their predoctoral dental education and indicated the need for further related education. There was 33% confusion with fluorosis and 16.6% with amelogenesis imperfecta. A total of 66.6% of the participants indicated that they require further education relating to MIH. Conclusion: Within the limitations of the present investigation, MIH awareness among the investigated groups varied but was highest amongst the pediatric residents.
Treatment Adherence Interventions for Burn Patients: What Works and What Role Can Motivational Interviewing Play?
Authors: Goans Christian R. R. , Meltzer Karen J. , Martin Blake , Roaten Kimberly
Published: April 2022

The unique challenges burn patients face along the trajectory of recovery necessitate an interdisciplinary team approach to care. As much as providers rely on care-team members for delivery of optimal treatment, the patient must be an active collaborator in their care. Optimal burn recovery outcomes hinge on treatment adherence. In addition to general challenges faced in ubiquity by burn patients, there are specific patient populations for whom treatment adherence is particularly challenging. Although psychological interventions have been used successfully with burn patients, very few are appropriate for both inpatient and outpatient care environments and most do not focus on treatment adherence. This paper reviews unique facets of Motivational Interviewing (MI) that may be applicable in interdisciplinary burn treatment teams across inpatient and outpatient settings to optimize treatment adherence.