bluuebunny/crossref_metadata_2025 huggingface dataset:
Short-term exposure to ambient temperature and the mortality burden of suicide in Japan
Authors: Thawonmas Ramita , Kim Yoonhee , Hashizume Masahiro
Published: May 2024

Background. Previous studies suggest that temperature may trigger suicide, with higher ambient temperatures associated with increased risk globally. However, quantification of the attributable suicide burden due to non-optimal temperatures, particularly across specific subgroups like sex, age, and method of suicide, is limited. This study aimed to quantify this attributable burden across Japan and assess variations in these subgroups to enhance understanding and identify high-risk populations. Methods. Data on daily suicide counts and mean temperatures were collected for all 47 prefectures in Japan (1973–2015) for a comprehensive, nationally representative analysis. Using time-stratified case-crossover analyses and a distributed lag non-linear model (DLNM), we estimated temperature-suicide associations, accounting for long-term trends, seasonality, and day-of-the-week effects, and capturing both nonlinear and delayed impacts of temperature. Overall attributable fractions and fractions attributable to cold (0th-10th percentile), cool (10th-50th), warm (50th-90th), and hot (90th-100th) temperatures were computed. Subgroup analyses were conducted by sex, age (0 – 64 years and ≥65 years), and method of suicide (violent or nonviolent). Results. Approximately 19.9% of suicides (95% empirical confidence interval (eCI): 18.8, 20.9) could be attributed to non-optimal temperatures, representing the proportion of cases that would not have occurred under optimal temperature conditions, identified at the temperature corresponding to the minimum risk of suicide. The highest fraction (9.9%, 95% eCI: 9.4, 10.4) was observed for warm temperatures (50th-90th percentile). Higher burdens were observed in females (23.7%), individuals aged ≥65 years (31.9%), and violent suicides (22.4%). Conclusions. Nearly 20% of suicides in Japan are associated with non-optimal temperatures, particularly during warmer conditions. These findings highlight the importance of integrating temperature considerations into suicide prevention strategies, particularly for vulnerable populations such as the elderly. While focused on Japan, these results advocate future research to explore similar burdens globally, enhancing our understanding of environmental impacts on suicide and informing targeted public health interventions.
Exploring dielectric properties in atomistic models of amorphous boron nitride
Authors: Galvani Thomas , Hamze Ali K , Caputo Laura , Kaya Onurcan , Dubois Simon M-M , Colombo Luigi , Nguyen Viet-Hung , Shin Yongwoo , Shin Hyeon-Jin , Charlier Jean-Christophe , Roche Stephan
Published: May 2024

We report a theoretical study of dielectric properties of models of amorphous Boron Nitride, using interatomic potentials generated by machine learning. We first perform first-principles simulations on small (about 100 atoms in the periodic cell) sample sizes to explore the emergence of mid-gap states and its correlation with structural features. Next, by using a simplified tight-binding electronic model, we analyse the dielectric functions for complex three dimensional models (containing about 10.000 atoms) embedding varying concentrations of sp1, sp2 and sp3 bonds between B and N atoms. Within the limits of these methodologies, the resulting value of the zero-frequency dielectric constant is shown to be influenced by the population density of such mid-gap states and their localization characteristics. We observe nontrivial correlations between the structure-induced electronic fluctuations and the resulting dielectric constant values. Our findings are however just a first step in the quest of accessing fully accurate dielectric properties of as-grown amorphous BN of relevance for interconnect technologies and beyond.
Electromechanical strain response of phosphorene nanotubes
Authors: Tran Kevin , Taylor Patrick D , Spencer Michelle J S
Published: May 2024

Nanomaterials that undergo structural or other property changes upon application of external stimuli are called stimuli responsive materials and are particularly suited for drug delivery, biosensing or artificial muscle applications. Two-dimensional (2D) black phosphorus is an ideal material for such applications due to its remarkable electromechanical response. Given that one-dimensional (1D) black phosphorus nanotubes (PNTs) are calculated to be energetically stable, it is possible that they can undergo similar electromechanical responses to their 2D counterparts, allowing their potential application as nanochannel devices for drug delivery. Using first-principles density functional theory, we investigated the electromechanical response of different-sized PNTs upon charge injection. Upon hole injection, the diameter of the PNTs expands up to a maximum of 30.2% for a (0,15) PNT that is 0.24 nm in diameter. The PNTs become highly p-doped as the valence band maximum crosses the Fermi level and undergoes switching from a direct to indirect band gap. The mechanism behind the electromechanical response was determined through analysis of the structural deformations, charge density distribution and Bader partial charges. It was shown that injection of charge alters the Young’s Modulus of the PNTs, as hole injection weakens the structural integrity of the nanotube, allowing a greater electromechanical response, with PNT-15 showing the largest decrease in the Young’s Modulus of 15.34%. These findings show that 1D PNTs are promising materials for the development of nanoelectromechanical actuators which could be used for drug delivery, energy harvesting or similar applications.
A rapid and quantitative absorption-based measurement to study the impact of antibiotics on bacterial growth
Authors: Meulebroeck Wendy , Thienpont Hugo , Goossens Herman , Moons Pieter , Ottevaere Heidi
Published: May 2024

Given the global rise in antimicrobial resistance levels, there is an urgent need to obtain the minimal inhibitory concentration (MIC) of an antibiotic as early as possible. In this paper, we present the first test results of a light-based concept where the interaction of a laser beam with the drug–bacterium sample is used to calculate MIC values within 6 h after cultivation. For this preliminary study, a total of 163 drug–bacterium pairs were tested and benchmarked with broth microdilution (BMD). The pathogen set included Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, Staphylococcus capitis, Staphylococcus cohnii, Staphylococcus epidermis, Staphylococcus haemolyticus, Enterococcus and Streptococcus pneumoniae. The selected drugs belonged to 10 different classes. The method under investigation showed a categorical concordance of 86.1% and an essential agreement of 80.3% with BMD. Due to its simplicity, the concept can be easily implemented on existing commercial platforms. This research shows promise for further studies potentially leading to a novel concept that can be employed to rapidly determine MIC values.
Solution-processing approach of nanomaterials toward an artificial sensory system
Authors: Song Okin , Cho Youngwook , Cho Soo-Yeon , Kang Joohoon
Published: May 2024

Artificial sensory systems have emerged as pivotal technologies to bridge the gap between the virtual and real-world, replicating human senses to interact intelligently with external stimuli. To practically apply artificial sensory systems in the real-world, it is essential to mass-produce nanomaterials with ensured sensitivity and selectivity, purify them for desired functions, and integrate them into large-area sensory devices through assembly techniques. A comprehensive understanding of each process parameter from material processing to device assembly is crucial for achieving a high-performing artificial sensory system. This review provides a technological framework for fabricating high-performance artificial sensory systems, covering material processing to device integrations. We introduce recent approaches for dispersing and purifying various nanomaterials including 0D, 1D, and 2D nanomaterials. We then highlight advanced coating and printing techniques of the solution-processed nanomaterials based on representative three methods including (i) evaporation-based assembly, (ii) assisted assembly, and (iii) direct patterning. We explore the application and performances of these solution-processed materials and printing methods in fabricating sensory devices mimicking five human senses including vision, olfaction, gustation, hearing, and tactile perception. Finally, we suggest an outlook for possible future research directions to solve the remaining challenges of the artificial sensory systems such as ambient stability, device consistency, and integration with AI-based software.
Parameter study and development of a warp knitting yarn compensation unit as basis for the realisation of contour-accurate non-crimp fabrics: a step towards for highly material efficient non-crimp fabrics
Authors: Zierold Konrad , Penzel Paul , Hahn Lars , Cherif Chokri
Published: May 2024

In response to the increasing demands for high-performance fiber-reinforced composites in structural lightweight construction, this study investigates the limitations of multiaxial non-crimp fabrics (NCF) and their semi-finished products. The current manufacturing constraints of NCF, limited to a constant working width, lead to oversizing in semi-finished textile products and significant material waste throughout the value chain. This study explores the development of NCF with variable area weights and thread densities as a potential solution.

The research described in this paper examines the effects of variable area weights and thread densities on textile behavior and warp knitting thread tension in the production of multiaxial NCF. The study focuses on varying key warp knitting parameters (stitch, knitting thread feed value, shape hole geometry), along with the measurement of the tensile force exerted on the warp knitting threads. Findings indicate a consistent increase in warp knitting thread tension in areas of reduced thread densities, unaffected by the fabric’s initial orientation. Higher initial yarn tension and increased yarn demand per stitch correlate with a greater tension increase in areas with lower thread density. This study proposes that refining stitching techniques and integrating adaptive yarn tension control modules could mitigate tension fluctuations and diminish fabric defects. These insights contribute to a better understanding of the material behavior of contour accurate NCF and their production. Coupled with the innovation of a warp knitting compensation unit, these findings mark a pivotal advancement toward producing contour accurate NCF in an inline and higly productive process technology, offering significant implications for the technical textile industry.
Soundscape mapping: understanding regional spatial and temporal patterns of soundscapes incorporating remotely-sensed predictors and wildfire disturbance
Authors: Quinn Colin A , Burns Patrick , Jantz Patrick , Salas Leonardo , Goetz Scott J , Clark Matthew L
Published: May 2024

Increased environmental threats require proper monitoring of animal communities to understand where and when changes occur. Ecoacoustic tools that quantify natural acoustic environments use a combination of biophony (animal sound) and geophony (wind, rain, and other natural phenomena) to represent the natural soundscape and, in comparison to anthropophony (technological human sound) can highlight valuable landscapes to both human and animal communities. However, recording these sounds requires intensive deployment of recording devices and storage and interpretation of large amounts of data, resulting in large data gaps across the landscape and periods in which recordings are absent. Interpolating ecoacoustic metrics like biophony, geophony, anthropophony, and acoustic indices can bridge these gaps in observations and provide insight across larger spatial extents and during periods of interest. Here, we use seven ecoacoustic metrics and acoustically-derived bird species richness across a heterogeneous landscape composed of densely urbanized, suburban, rural, protected, and recently burned lands in Sonoma County, California, U.S.A., to explore spatiotemporal patterns in ecoacoustic measurements. Predictive models of ecoacoustic metrics driven by land-use/land-cover, remotely-sensed vegetation structure, anthropogenic impact, climate, geomorphology, and phenology variables capture landscape and daily differences in ecoacoustic patterns with varying performance (avg. R
2 = 0.38 ± 0.11) depending on metric and period-of-day and provide interpretable patterns in sound related to human activity, weather phenomena, and animal activity. We also offer a case study on the use of the data-driven prediction of biophony to capture changes in soniferous species activity before (1–2 years prior) and after (1–2 years post) wildfires in our study area and find that biophony may depict the reorganization of acoustic communities following wildfires. This is demonstrated by an upward trend in activity 1–2 years post-wildfire, particularly in more severely burned areas. Overall, we provide evidence of the importance of climate, spaceborne-lidar-derived forest structure, and phenological time series characteristics when modeling ecoacoustic metrics to upscale site observations and map ecoacoustic biodiversity in areas without prior acoustic data collection. Resulting maps can identify areas of attention where changes in animal communities occur at the edge of human and natural disturbances.
Epistemology, Ethics, Politics
Authors: Foley Richard
Published: May 2024

A standard recommendation of misinformation experts for dealing with individuals with preposterous, toxic views on important topics is to search for commonalities as opposed to dwelling on differences. The hope is that the commonalities can be used to reveal cracks in the protective shell they have erected around their views, which can then be expanded to get them to set aside defensive rationalizations. The unsettling reality, however, is that at least some cases, the outlandish, dangerous views of these individuals may so tightly cohere with their other belief-like attitudes and values that no combination of truth-related and non-truth-related considerations is capable of changing their minds. If so, the only remaining option is to find ways of limiting the damage even if these may involve concessions with values we hold dear. Resorting to non-argumentative tactics is disquieting, but extremist, dogmatic challenges arise in epistemology just as they do in ethics and politics, and the resources for dealing with them are likewise similar. The challenges cannot be tackled in the uncluttered domain imagined by the standard view, where the only relevant values are those of now having accurate beliefs and now not having inaccurate ones. Whatever responses there may be have to be found in the messy region shared by epistemology, ethics, and politics.
Identifying the relation between food groups and biological ageing: a data-driven approach
Authors: Biemans Ynte , Bach Daimy , Behrouzi Pariya , Horvath Steve , Kramer Charlotte S , Liu Simin , Manson JoAnn E , Shadyab Aladdin H , Stewart James , Whitsel Eric A , Yang Bo , de Groot Lisette , Grootswagers Pol
Published: May 2024

Background

Heterogeneity in ageing rates drives the need for research into lifestyle secrets of successful agers. Biological age, predicted by epigenetic clocks, has been shown to be a more reliable measure of ageing than chronological age. Dietary habits are known to affect the ageing process. However, much remains to be learnt about specific dietary habits that may directly affect the biological process of ageing.

Objective

To identify food groups that are directly related to biological ageing, using Copula Graphical Models.

Methods

We performed a preregistered analysis of 3,990 postmenopausal women from the Women’s Health Initiative, based in North America. Biological age acceleration was calculated by the epigenetic clock PhenoAge using whole-blood DNA methylation. Copula Graphical Modelling, a powerful data-driven exploratory tool, was used to examine relations between food groups and biological ageing whilst adjusting for an extensive amount of confounders. Two food group–age acceleration networks were established: one based on the MyPyramid food grouping system and another based on item-level food group data.

Results

Intake of eggs, organ meat, sausages, cheese, legumes, starchy vegetables, added sugar and lunch meat was associated with biological age acceleration, whereas intake of peaches/nectarines/plums, poultry, nuts, discretionary oil and solid fat was associated with decelerated ageing.

Conclusion

We identified several associations between specific food groups and biological ageing. These findings pave the way for subsequent studies to ascertain causality and magnitude of these relationships, thereby improving the understanding of biological mechanisms underlying the interplay between food groups and biological ageing.
Biomarker signatures associated with ageing free of major chronic diseases: results from a population-based sample of the EPIC-Potsdam cohort
Authors: Reichmann Robin , Schulze Matthias B , Pischon Tobias , Weikert Cornelia , Aleksandrova Krasimira
Published: May 2024

Background

A number of biomarkers denoting various pathophysiological pathways have been implicated in the aetiology and risk of age-related diseases. Hence, the combined impact of multiple biomarkers in relation to ageing free of major chronic diseases, such as cancer, cardiovascular disease and type 2 diabetes, has not been sufficiently explored.

Methods

We measured concentrations of 13 biomarkers in a random subcohort of 2,500 participants in the European Prospective Investigation into Cancer and Nutrition Potsdam study. Chronic disease-free ageing was defined as reaching the age of 70 years within study follow-up without major chronic diseases, including cardiovascular disease, type 2 diabetes or cancer. Using a novel machine-learning technique, we aimed to identify biomarker clusters and explore their association with chronic disease-free ageing in multivariable-adjusted logistic regression analysis taking socio-demographic, lifestyle and anthropometric factors into account.

Results

Of the participants who reached the age of 70 years, 321 met our criteria for chronic-disease free ageing. Machine learning analysis identified three distinct biomarker clusters, among which a signature characterised by high concentrations of high-density lipoprotein cholesterol, adiponectin and insulin-like growth factor-binding protein 2 and low concentrations of triglycerides was associated with highest odds for ageing free of major chronic diseases. After multivariable adjustment, the association was attenuated by socio-demographic, lifestyle and adiposity indicators, pointing to the relative importance of these factors as determinants of healthy ageing.

Conclusion

These data underline the importance of exploring combinations of biomarkers rather than single molecules in understanding complex biological pathways underpinning healthy ageing.
The effect of increased fruit and vegetable consumption on blood pressure and lipids: a pooled analysis of six randomised controlled fruit and vegetable intervention trials
Authors: Elsahoryi Nour A , Neville Charlotte E , Patterson Chris C , McKinley Michelle C , Baldrick Francina R , Mulligan Ciara , McCall Damian O , Noad Rebecca L , Rooney Ciara , Wallace Ian , McEvoy Claire T , Hunter Steven , McCance David R , Edgar David J , Elborn Stuart J , McKeown Pascal P , Young Ian S , Moore Rachel E , Nugent Anne P , Woodside Jayne V
Published: May 2024

Background

Increasing fruit and vegetable (FV) consumption is associated with reduced cardiovascular disease risk in observational studies but with little evidence from randomised controlled trials (RCTs). The impact of concurrent pharmacological therapy is unknown.

Objective

To pool data from six RCTs to examine the effect of increasing FV intake on blood pressure (BP) and lipid profile, also exploring whether effects differed by medication use.

Design

Across trials, dietary intake was assessed by diet diaries or histories, lipids by routine biochemical methods and BP by automated monitors. Linear regression provided an estimate of the change in lipid profile or BP associated with a one portion increase in self-reported daily FV intake, with interaction terms fitted for medication use.

Results

The pooled sample included a total of 554 participants (308 males and 246 females). Meta-analysis of regression coefficients revealed no significant change in either systolic or diastolic BP per portion FV increase, although there was significant heterogeneity across trials for systolic BP (I2 = 73%). Neither adjusting for change in body mass index, nor analysis according to use of anti-hypertensive medication altered the relationship. There was no significant change in lipid profile per portion FV increase, although there was a significant reduction in total cholesterol among those not on lipid-lowering therapy (P < 0.05 after Bonferroni correction).

Conclusion

Pooled analysis of six individual FV trials showed no impact of increasing intake on BP or lipids, but there was a total cholesterol-lowering effect in those not on lipid-lowering therapy.
A rank-based sequential test of independence
Authors: Henzi Alexander , Law Michael
Published: May 2024

We consider the problem of independence testing for two univariate random variables in a sequential setting. By leveraging recent developments on safe, anytime-valid inference, we propose a test with time-uniform Type-I error control and derive explicit bounds on the finite-sample performance of the test. We demonstrate the empirical performance of the procedure in comparison to existing sequential and nonsequential independence tests. Furthermore, since the proposed test is distribution-free under the null hypothesis, we empirically simulate the gap due to Ville’s inequality, the supermartingale analogue of Markov’s inequality, that is commonly applied to control Type-I error in anytime-valid inference, and apply this to construct a truncated sequential test.
Architectural distortion on digital breast tomosynthesis mammograms in symptomatic breast clinics: what are the result outcomes?
Authors: Bansal Gaurav J , Kale Riya
Published: May 2024

Objectives

In April 2020, standard two-dimensional (2D) full-field digital mammograms were replaced with digital breast tomosynthesis (DBT) and synthesised 2D views for symptomatic breast clinics. This study aimed to evaluate the positive predictive value (PPV) for malignancy in DBT-detected Architectural distortion (AD).

Methods

All mammogram reports with the word “distortion” were assessed between April 2020 and October 2022. There were 458 mammograms with the word “distortion.” After excluding mammograms with no distortion (n = 128), post-surgical distortion (n = 173), distortion with mass (n = 33), and unchanged distortion (n = 14), there were 111 patients with pure distortion. Correlation with histopathology was obtained where possible. All patients were followed for a minimum of 2 years.

Results

Forty-two out of 111 patients (37.84%) with AD had a normal ultrasound (US) and were discharged. Fifty-five (49.5%) patients had sonographic correlation corresponding to the distortion, leading to US-guided biopsy. Thirteen (23.6%) had tomosynthesis-guided biopsy, and one had a skin biopsy. The PPV for malignancy was 42.34%. Malignancy diagnoses were higher with US-guided biopsies than tomosynthesis-guided biopsies, 78.1% and 30%, respectively.

Conclusion

With a total malignancy rate of 42.34%, DBT-detected AD has a high enough PPV for malignancy to justify selective tissue sampling if a sonographic correlate is present or with suspicious mammograms. The chances of malignancy are higher when a sonographic correlate corresponding to AD is present.

Advances in knowledge

AD on DBT/synthesized mammograms views in symptomatic breast clinic patients justifies selective sampling.
The vascular contribution of apolipoprotein E to Alzheimer’s disease
Authors: Chen Feng , Zhao Jing , Meng Fanxia , He Fangping , Ni Jie , Fu Yuan
Published: May 2024

Alzheimer’s disease, the most prevalent form of dementia, imposes a substantial societal burden. The persistent inadequacy of disease-modifying drugs targeting amyloid plaques and neurofibrillary tangles suggests the contribution of alternative pathogenic mechanisms. A frequently overlooked aspect is cerebrovascular dysfunction, which may manifest early in the progression of Alzheimer’s disease pathology. Mounting evidence underscores the pivotal role of the apolipoprotein E gene, particularly the apolipoprotein ε4 allele as the strongest genetic risk factor for late-onset Alzheimer’s disease, in the cerebrovascular pathology associated with Alzheimer’s disease.

In this review, we examine the evidence elucidating the cerebrovascular impact of both central and peripheral apolipoprotein E on the pathogenesis of Alzheimer’s disease. We present a novel three-hit hypothesis, outlining potential mechanisms that shed light on the intricate relationship among different pathogenic events. Finally, we discuss prospective therapeutics targeting the cerebrovascular pathology associated with apolipoprotein E and explore their implications for future research endeavours.
Navigating gender hate in manospheres: women’s affective dissonance and refusal on LIHKG in the 2019 Hong Kong anti-extradition bill movement
Authors: Wong Cecilia K H
Published: May 2024

This article examines women’s dissonance and refusal of gender hate on LIHKG, a platform converged with local and Global North manospheres that gained prominence for political communication during Hong Kong’s Anti-Extradition Law Amendment Bill movement. Despite criticism for its misogyny, LIHKG witnessed women’s participation during the movement. However, women’s agencies are underexplored by its critics and studies on the manosphere. This study argues that acknowledging dissonance within manospheres redirects focus on the targets of gender hate, specifically women, accentuating the possibilities of feminist intervention within gender hate networks. Employing a digital ethnographic approach, this study contributes by: (1) providing a relational and contextualized understanding of the convergence between manospheres beyond the notion of homogenization; (2) redressing the injustice in hate which obliterates the hated bodies; and (3) breaking the unintended repetition of such injustice in the literature on the manosphere, which has paid little attention on the repugnant women’s agencies.
Delineating memory reactivation in sleep with verbal and non-verbal retrieval cues
Authors: Guttesen Anna á V , Denis Dan , Gaskell M Gareth , Cairney Scott A
Published: May 2024

Sleep supports memory consolidation via the reactivation of newly formed memory traces. One way to investigate memory reactivation in sleep is by exposing the sleeping brain to auditory retrieval cues; a paradigm known as targeted memory reactivation. To what extent the acoustic properties of memory cues influence the effectiveness of targeted memory reactivation, however, has received limited attention. We addressed this question by exploring how verbal and non-verbal memory cues affect oscillatory activity linked to memory reactivation in sleep. Fifty-one healthy male adults learned to associate visual stimuli with spoken words (verbal cues) and environmental sounds (non-verbal cues). Subsets of the verbal and non-verbal memory cues were then replayed during sleep. The voice of the verbal cues was either matched or mismatched to learning. Memory cues (relative to unheard control cues) prompted an increase in theta/alpha and spindle power, which have been heavily implicated in sleep-associated memory processing. Moreover, verbal memory cues were associated with a stronger increase in spindle power than non-verbal memory cues. There were no significant differences between the matched and mismatched verbal cues. Our findings suggest that verbal memory cues may be most effective for triggering memory reactivation in sleep, as indicated by an amplified spindle response.
Enhanced Tuberculosis Diagnosis With Computer-aided Chest X-ray and Urine Lipoarabinomannan in Adults With Human Immunodeficiency Virus Admitted to Hospital (CASTLE Study): A Cluster Randomized Trial
Authors: Burke Rachael M , Nyirenda Saulos K , Mtenga Timeo , Twabi Hussein H , Joekes Elizabeth , Walker Naomi F , Nyirenda Rose , Gupta-Wright Ankur , Nliwasa Marriott , Fielding Katherine , MacPherson Peter , Corbett Elizabeth L
Published: May 2024

Background

People with human immunodeficiency virus (PHIV) admitted to the hospital have high mortality, with tuberculosis (TB) being the major cause of death. Systematic use of new TB diagnostics could improve TB diagnosis and might improve outcomes.

Methods

We conducted a cluster randomized trial among adult PHIV admitted to Zomba Central Hospital, Malawi. Admission days were randomly assigned to: enhanced TB diagnostics using urine lipoarabinomannan (LAM) antigen tests (SILVAMP-LAM, Fujifilm, Japan and Determine-LAM, Alere/Abbot, USA), digital chest X-ray with computer-aided diagnosis (dCXR-CAD, CAD4TBv6, Delft, Netherlands), plus usual care (“enhanced TB diagnostics”); or usual care alone (“usual care”). The primary outcome was TB treatment initiation during admission. Secondary outcomes were 56-day mortality, TB diagnosis within 24 hours, and undiagnosed TB at discharge, ascertained by culture of one admission sputum sample.

Findings

Between 2 September 2020 and 15 February 2022, we recruited 419 people. Four were excluded postrecruitment, leaving 415 adults recruited during 207 randomly assigned admission days in modified intention-to-treat analysis. At admission, 90.8% (377/415) were taking antiretroviral therapy with a median CD4 cell count of 240 cells/mm3. In the enhanced diagnostic arm, median CAD4TBv6 score was 60 (interquartile range: 51–71), 4.4% (9/207) had SILVAMP-LAM–positive and 14.4% (29/201) had Determine-LAM–positive urine with 3 samples positive by both urine tests. TB treatment was initiated in 46/207 (22.2%) in the enhanced TB diagnostics arm and 24/208 (11.5%) in the usual care arm (risk ratio, 1.92; 95% confidence interval [CI]: 1.20–3.08). There was no difference in mortality by 56 days (enhanced TB diagnosis: 54/207, 26.1%; usual care: 52/208, 25.0%; hazard ratio. 1.05; 95% CI: .72–1.53); TB treatment initiation within 24 hours (enhanced TB diagnosis: 8/207, 3.9%; usual care: 5/208, 2.4%; risk ratio, 1.61; 95% CI: .53–4.71); or undiagnosed microbiological-confirmed TB at discharge (enhanced TB diagnosis, 0/207 [0.0%], usual care arm 2/208 [1.0%]; P = .50.

Interpretation

Urine SILVAMP-LAM/Determine-LAM plus dCXR-CAD diagnostics identified more hospitalized PHIV with TB than usual care. The increase in TB treatment appeared mainly because of greater use of Determine-LAM, rather than SILVAMP-LAM or dCXR-CAD. Poor concordance between Determine-LAM and SILVAMP-LAM urine tests requires further investigation. Inpatient mortality for adults with human immunodeficiency virus remains unacceptability high.
Radiomics-Based Analysis of Intestinal Ultrasound Images for Inflammatory Bowel Disease: A Feasibility Study
Authors: Gu Phillip , Chang Jui-Hsuan , Carter Dan , McGovern Dermot P B , Moore Jason , Wang Paul , Huang Xiuzhen
Published: May 2024

Background

The increasing adoption of intestinal ultrasound (IUS) for monitoring inflammatory bowel diseases (IBD) by IBD providers has uncovered new challenges regarding standardized image interpretation and limitations as a research tool. Artificial intelligence approaches can help address these challenges. We aim to determine the feasibility of radiomic analysis of IUS images and to determine if a radiomics-based classification model can accurately differentiate between normal and abnormal IUS images. We will also compare the radiomic-based model’s performance to a convolutional neural network (CNN)-based classification model to understand which method is more effective for extracting meaningful information from IUS images.

Methods

Retrospectively analyzing IUS images obtained during routine outpatient visits, we developed and tested radiomic-based and CNN-based models to distinguish between normal and abnormal images, with abnormal images defined as bowel wall thickness > 3 mm or bowel hyperemia with modified Limberg score ≥ 1 (both are surrogate markers for inflammation). Model performances were measured by area under the receiver operator curve (AUC).

Results

For this feasibility study, 125 images (33% abnormal) were analyzed. A radiomic-based model using XG boost yielded the best classifier model with average test AUC 0.98%, 93.8% sensitivity, 93.8% specificity, and 93.7% accuracy. The CNN-based classification model yielded an average testing AUC of 0.75.

Conclusions

Radiomic analysis of IUS images is feasible, and a radiomic-based classification model could accurately differentiate abnormal from normal images. Our findings establish methods to facilitate future radiomic-based IUS studies that can help standardize image interpretation and expand IUS research capabilities.
Safety and Effectiveness of Ustekinumab for Crohn’s Disease With Perianal Manifestations: Ad hoc Analysis Data From 1-Year Post-Marketing Surveillance Study in Japan
Authors: Nagano Katsumasa , Tsuchiya Hiroaki , Asano Teita , Yamazaki Hiroshi , Tominaga Sonoko , Matsumoto Takayuki
Published: May 2024

Background

Crohn’s disease (CD) is an immune-mediated inflammatory disorder of the gastrointestinal tract with perianal disease being one of the challenging possible manifestations. Here, we report, an ad hoc analysis of the safety and effectiveness of 1-year use of ustekinumab (UST) for CD in patients with perianal manifestations using post-marketing surveillance (PMS) data in Japan.

Methods

Among 341 patients enrolled in the PMS, 229 and 224 patients who had baseline Crohn’s Disease Activity Index (CDAI) data used for evaluating perianal manifestations were included in the safety and efficacy analysis sets, respectively. Incidence of adverse drug reactions, clinical remission, the mean or its change in CDAI scores, and CDAI items were evaluated through week 52 in the presence or absence of perianal manifestations at baseline. The prevalence of perianal manifestations was also described.

Results

Comparing patients with and without baseline perianal manifestations at week 52, there was no difference in ADR incidence (9.1% [n = 66] vs. 15.3% [n = 163]), no difference in clinical remission (68.3% vs. 59.9%; P = 0.269), and decreased mean change of CDAI score (−82.9 [n = 60] vs. −68.8 [n = 137]). The proportion of patients with perianal manifestations decreased after UST treatment in both biologics-naïve patients (23.5% [n = 4/17]) and patients who had received biologics (35.0% [n = 14/40]) at week 52.

Conclusions

In Japanese clinical practice, UST is safe and effective in CD patients with and without perianal manifestations. The therapy might be also beneficial in those with manifestations regardless of prior use of other biologics.
Genome-wide DNA Methylome and Transcriptome Profiling Reveals Key Genes Involved in the Dysregulation of Adipose Stem Cells in Crohn’s Disease
Authors: Monfort-Ferré Diandra , Boronat-Toscano Albert , Sánchez-Herrero José-Francisco , Caro Aleidis , Menacho Margarita , Vañó-Segarra Irene , Martí Marc , Espina Beatriz , Pluvinet Raquel , Cabrinety Lidia , Abadia Carme , Ejarque Miriam , Nuñez-Roa Cati , Maymo-Masip Elsa , Sumoy Lauro , Vendrell Joan , Fernández-Veledo Sonia , Serena Carolina
Published: May 2024

Background and Aims

Crohn’s disease [CD] is characterised by the expansion of mesenteric adipose tissue [MAT], named creeping fat [CF], which seems to be directly related to disease activity. Adipose-stem cells [ASCs] isolated from the CF of patients with CD are extremely pro-inflammatory, which persists during disease remission. We hypothesised that the dysfunctional ASCs in CD accumulate epigenetic modifications triggered by the inflammatory environment, that could serve as molecular markers.

Methods

Genome-wide DNA methylome and transcriptome profiling were performed in ASCs isolated from MAT biopsies of patients with active and inactive disease and from non-Crohn’s disease patients [non-CD]. A validation cohort was used to test the main candidate genes via quantitative polymerase chain reaction in other fat depots and immune cells.

Results

We found differences in DNA methylation and gene expression between ASCs isolated from patients with CD and from non-CD subjects, but we found no differences related to disease activity. Pathway enrichment analysis revealed that oxidative stress and immune response were significantly enriched in active CD, and integration analysis identified MAB21L2, a cell fate-determining gene, as the most affected gene in CD. Validation analysis confirmed the elevated gene expression of MAB21L2 in MAT and in adipose tissue macrophages in active CD. We also found a strong association between expression of the calcium channel subunit gene CACNA1H and disease remission, as CACNA1H expression was higher in ASCs and MAT from patients with inactive CD, and correlates negatively with C-reactive protein in peripheral blood mononuclear cells.

Conclusion

We identified a potential gene signature of CD in ASCs obtained from MAT. Integration analysis highlighted two novel genes demonstrating a negative correlation between promoter DNA methylation and transcription: one linked to ASCs in CD [MAB21L2] and the other [CACNA1H] related to disease remission.
Sequential trait evolution did not drive deep-time diversification in sharks
Authors: Marion Alexis F P , Condamine Fabien L , Guinot Guillaume
Published: May 2024

Estimating how traits evolved and impacted diversification across the tree of life represents a critical topic in ecology and evolution. Although there has been considerable research in comparative biology, large parts of the tree of life remain underexplored. Sharks are an iconic clade of marine vertebrates, and key components of marine ecosystems since the early Mesozoic. However, few studies have addressed how traits evolved or whether they impacted their extant diversity patterns. Our study aimed to fill this gap by reconstructing the largest time-calibrated species-level phylogeny of sharks and compiling an exhaustive database for ecological (diet, habitat) and biological (reproduction, maximum body length) traits. Using state-of-the-art models of evolution and diversification, we outlined the major character shifts and modes of trait evolution across shark species. We found support for sequential models of trait evolution and estimated a small to medium-sized lecithotrophic and coastal-dwelling most recent common ancestor for extant sharks. However, our exhaustive hidden traits analyses do not support trait-dependent diversification for any examined traits, challenging previous works. This suggests that the role of traits in shaping sharks’ diversification dynamics might have been previously overestimated and should motivate future macroevolutionary studies to investigate other drivers of diversification in this clade.
Quorum sensing-related activities of beneficial and pathogenic bacteria have important implications for plant and human health
Authors: Hartmann Anton , Binder Tatiana , Rothballer Michael
Published: May 2024

Eukaryotic organisms coevolved with microbes from the environment forming holobiotic meta-genomic units. Members of host-associated microbiomes have commensalic, beneficial/symbiotic, or pathogenic phenotypes. More than 100 years ago, Lorenz Hiltner, pioneer of soil microbiology, introduced the term ‘Rhizosphere’ to characterize the observation that a high density of saprophytic, beneficial, and pathogenic microbes are attracted by root exudates. The balance between these types of microbes decide about the health of the host. Nowadays we know, that for the interaction of microbes with all eukaryotic hosts similar principles and processes of cooperative and competitive functions are in action. Small diffusible molecules like (phyto)hormones, volatiles and quorum sensing signals are examples for mediators of interspecies and cross-kingdom interactions. Quorum sensing of bacteria is mediated by different autoinducible metabolites in a density-dependent manner. In this perspective publication, the role of QS-related activities for the health of hosts will be discussed focussing mostly on N-acyl-homoserine lactones (AHL). It is also considered that in some cases very close phylogenetic relations exist between plant beneficial and opportunistic human pathogenic bacteria. Based on a genome and system-targeted new understanding, sociomicrobiological solutions are possible for the biocontrol of diseases and the health improvement of eukaryotic hosts.
The majority of fact-checking labels in the United States are intense and this decreases engagement intention
Authors: Xue Haoning , Zhang Jingwen , Shen Cuihua , Wojcieszak Magdalena
Published: May 2024

Fact-checking labels have been widely accepted as an effective misinformation correction method. However, there is limited theoretical understanding of fact-checking labels’ impact. This study theorizes that language intensity influences fact-checking label processing and tests this idea through a multi-method design. We first rely on a large-scale observational dataset of fact-checking labels from 7 U.S. fact-checking organizations (N = 33,755) to examine the labels’ language intensity and then use a controlled online experiment in the United States (N = 656) to systematically test the causal effects of fact-checking label intensity (low, moderate, or high) and fact-checking source (professional journalists or artificial intelligence) on perceived message credibility of and the intention to engage with fact-checking messages. We found that two-thirds of existing labels were intense. Such high-intensity labels had null effects on messages’ perceived credibility, yet decreased engagement intention, especially when labels were attributed to AI. Using more intense labels may not be an effective fact-checking approach.
Optimizing patient outcomes: the impact of multimodal preemptive analgesia in video-assisted thoracoscopic lobectomy
Authors: Li Bing , Chen Yu , Ma Rong
Published: May 2024

OBJECTIVES

The aim of this study was to evaluate the efficacy of a multimodal preemptive analgesia management approach, specifically incorporating ultrasound-guided thoracic paravertebral block (UG-TPVB) in conjunction with intravenous analgesia, after video-assisted thoracoscopic (VATS) lobectomy under the guidance of enhanced recovery after surgery.

METHODS

A total of 690 patients who underwent VATS lobectomy between October 2021 and March 2022 were divided into the UG-TPVB group (group T, n = 345) and the control group (group C, n = 345). Patients in group T received UG-TPVB prior to the induction of general anaesthesia, while group C did not undergo nerve block. A comparison was conducted between the 2 groups regarding various indicators, including postoperative sedation, static/dynamic numeric rating scale scores, intraoperative fentanyl consumption, duration of mechanical ventilation/anaesthesia recovery/hospitalization, postoperative complications and other relevant factors.

RESULTS

The static/dynamic numeric rating scale scores of group T were lower than those of group C after surgery. Intraoperative fentanyl consumption in group T (0.384 ± 0.095 mg) was lower than that in group C (0.465 ± 0.053 mg). The duration of mechanical ventilation, anaesthesia recovery and hospitalization were significantly shorter in group T compared to group C. Patient satisfaction rate in group T (70.1%) was higher than that in group C (53.6%). All differences were statistically significant (P < 0.05).

CONCLUSIONS

The multimodal preemptive analgesia management strategy effectively reduces postoperative pain, decreases opioid consumption and promotes faster recovery in patients undergoing VATS lobectomy.
Conservative Worldviews and the Climate Publics of New Zealand and Australia
Authors: Crawley Sam
Published: May 2024

Public opinion on climate change is complex, comprising aspects such as belief, support for policies, and issue salience. This multidimensional nature of climate opinion can be accounted for by identifying “publics,” each comprising people with similar climate attitudes. Climate opinion has also been found to relate strongly to political worldviews, with people holding conservative attitudes tending to have low levels of engagement with climate change. However, few studies have investigated how political worldviews relate to membership of climate publics. The current study therefore identifies climate publics in New Zealand and Australia and investigates how they relate to economic and social aspects of conservative worldviews. Using an original online survey, I find no clear link between economic conservatism and membership of climate publics. Social conservatives, however, are more likely to be members of publics with low levels of climate engagement and of “middle” publics, which accept the science of climate change but are “lukewarm” on climate action. Climate opinion appears to be more polarized in Australia than New Zealand. These results provide insight into the worldviews of people in the “mainstream” of climate opinion and suggest that only a minority of the public are demanding ambitious climate action.
Happy Click!: Investigating the Use of a Tangible Interface to Facilitate the Three Good Things Positive Psychology Intervention
Authors: Siriaraya Panote , Tanaka Ryotaro , She Wan Jou , Jain Rushil , Schok Michaela , De Ruiter Martine , Desmet Pieter , Nakajima Shinsuke
Published: May 2024

The ‘Three Good Things’ (TGT), a structured journaling exercise, has shown great effect in helping improve mental well-being. However, the exercise could be quite tedious and difficult to carry out daily. To improve the user experience of the exercise, we developed ‘Happy Click’, a mobile application which makes use of a physically present smart button to enable users to record their daily TGT in a more engaging manner. Two preliminary exploratory studies were carried out to examine the feasibility of our approach where the effects of the TGT intervention designed around the mobile and tangible happy click concepts were examined. The findings suggested that participants had significantly less stress after carrying out the TGT using the tangible interface. Participants also reported better user experience and acceptance when carrying out the exercise using the tangible interface compared to the mobile and higher levels of attractiveness and stimulation with the mobile TGT compared to the traditional approach. While these promising results should be interpreted with caution due to the limited sample size, they also underscore the potential advantages of our ‘Happy click’ approach and highlight the need for further, larger-scale evaluations to be conducted to validate its efficacy and scalability.