bluuebunny/crossref_metadata_2025 huggingface dataset:
A Numerical Simulation of Moisture Reduction in Fine Soil Subgrade with Wicking Geotextiles
Authors: Ma Chuanyi , Feng Haojie , Wang Chuan , Zhang Ning , Liu Yiyi , Li Jinglei , Liu Xia , Li Shasha , Jiang Hongguang , Li Yixin
Published: January 2024

A new wicking geotextile is proposed to control the water content of fine-grained soil subgrade. By comparing the spatial distribution of volumetric water content and matric suction before and after the installation of the wicking geotextile, the effectiveness of the geotextile in controlling the subgrade humidity is evaluated. Firstly, the hydraulic parameters of the wicking geotextile are obtained through laboratory tests using a pressure plate apparatus. Then, a numerical model for water flow in the subgrade is established using COMSOL to obtain the spatial distribution characteristics of humidity in the subgrade under different groundwater levels (2~8 m). The results show the wicking geotextile exhibits strong hydrophilicity, low water retention, and high horizontal permeability. Compared to the subgrade without geotextile, the water content of the soil above the geotextile decreases significantly by 7.6~9.6% at groundwater levels of 4~8m, while the saturation decreases by 18.3~23.0%, and the matric suction increases by 2~2.3 times. The wicking fabric functions as an effective drainage material to serve as a capillary barrier in the cross-plane direction and an effective drainage tunnel to transport water in the in-plane direction. The dynamic resilient modulus of the subgrade increases by 23.2~43.6%. The wicking geotextile effectively absorbs and drains weakly bound water in unsaturated soil due to the matric suction difference and its horizontal drainage capacity to improve the bearing capacity of the subgrade. It suggests that using wicking geotextile for drainage and reinforcement in fine-grained soil subgrades with groundwater levels ranging from 4 to 8 m is beneficial.
Adaptive Graph Attention and Long Short-Term Memory-Based Networks for Traffic Prediction
Authors: Zhu Taomei , Boada Maria Jesus Lopez , Boada Beatriz Lopez
Published: January 2024

While the increased availability of traffic data is allowing us to better understand urban mobility, research on data-driven and predictive modeling is also providing new methods for improving traffic management and reducing congestion. In this paper, we present a hybrid predictive modeling architecture, namely GAT-LSTM, by incorporating graph attention (GAT) and long short-term memory (LSTM) networks for handling traffic prediction tasks. In this architecture, GAT networks capture the spatial dependencies of the traffic network, LSTM networks capture the temporal correlations, and the Dayfeature component incorporates time and external information (such as day of the week, extreme weather conditions, holidays, etc.). A key attention block is designed to integrate GAT, LSTM, and the Dayfeature components as well as learn and assign weights to these different components within the architecture. This method of integration is proven effective at improving prediction accuracy, as shown by the experimental results obtained with the PeMS08 open dataset, and the proposed model demonstrates state-of-the-art performance in these experiments. Furthermore, the hybrid model demonstrates adaptability to dynamic traffic conditions, different prediction horizons, and various traffic networks.
The Prevalence, Epidemiological, and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) in Macau (2017–2022)
Authors: Xing Abao , Ng Hoi Man , Jiao Huining , Li Kefeng , Ye Qianhong
Published: January 2024

Macau, recognized as a global tourism hub and the world’s most densely populated region, provides a unique environment conducive to methicillin-resistant Staphylococcus aureus (MRSA) transmission in healthcare and community settings, posing a significant public health concern both locally and globally. The epidemiology and molecular characteristics of MRSA in the distinct city of Macau remain largely unelucidated. This five-year longitudinal study (2017–2022) examined the local prevalence and molecular typing of MRSA in Macau, with future MRSA type distribution predicted through ARIMA modeling. We subsequently analyzed the epidemiological characteristics of MRSA, including specimen source, clinical department, collection year, season, patient age, sex, and the annual number of tourists. Comprehensive antibiotic resistance profiles of the strains were also assessed. Of 504 clinically isolated S. aureus strains, 183 (36.3%) were identified as MRSA by the cefoxitin disk diffusion method and validated through multi-locus sequence typing (MLST). The MRSA detection rate showed an upward trend, increasing from 30.1% in 2017 to 45.7% in 2022. SCCmec type IV was predominant (28.9%), followed by types II (25.4%), III (22.1%), and V (22.1%). The primary sources of MRSA isolates were sputum (39.2%) and secretions (25.6%). Older age emerged as a risk factor for MRSA infection, whereas no significant associations were found with seasonal variations, gender, or the annual number of tourists. Despite displaying universal resistance to cefoxitin, oxacillin, and benzylpenicillin, MRSA isolates in Macau remained fully sensitive to vancomycin, tigecycline, quinupristin, nitrofurantoin, and linezolid. Continuous surveillance and analysis of MRSA distribution in Macau could provide invaluable insights for the effective management of MRSA prevention and control measures within healthcare settings.
Evaluation of Biofilm Production and Antifungal Susceptibility to Fluconazole in Clinical Isolates of Candida spp. in Both Planktonic and Biofilm Form
Authors: Marzucco Anna , Gatti Giulia , Montanari Maria Sofia , Fantini Michela , Colosimo Claudia , Tamburini Maria Vittoria , Arfilli Valentina , Morotti Manuela , Schiavone Pasqualina , Congestrì Francesco , Manera Martina , Denicolò Agnese , Brandolini Martina , Taddei Francesca , Grumiro Laura , Zannoli Silvia , Dirani Giorgio , De Pascali Alessandra Mistral , Sambri Vittorio , Cricca Monica
Published: January 2024

Candida spp. are an important opportunistic pathogen that can represent a possible cause of severe infections, especially in immunocompromised individuals. The clinical impact of Candida spp. depends, in part, on the ability to form biofilms, communities of nestled cells into the extracellular matrix. In this study, we compared the biofilm formation ability of 83 strains of Candida spp. isolated from blood cultures and other materials, such as respiratory samples, urine, and exudate, and their sensitivity to fluconazole (FLZ). Strains were divided into tertiles to establish cut-offs to classify isolates as low, moderate, or high biofilm producers (<0.26, 0.266–0.839, >0.839) and biofilms with low, moderate, or high metabolic activity (<0.053, 0.053–0.183, >0.183). A non-linear relationship between biofilm production and metabolic activity was found in C. glabrata and C. tropicalis. In addition, the increase in minimum biofilm eradication concentrations (MBEC50) compared to the Minor Inhibitory Concentration (PMIC) of the planktonic form in Candida spp. confirms the role of biofilm in the induction of resistance to FLZ.
Rapid Visual Detection of Methicillin-Resistant Staphylococcus aureus in Human Clinical Samples via Closed LAMP Assay Targeting mecA and spa Genes
Authors: Abusheraida Noora S. A. , AlBaker Asraa A. H. , Aljabri Asmaa S. A. , Abdelrahman Hana A. , Al-Mana Hassan , Wilson Godwin J. , Anan Khalid A. , Eltai Nahla O.
Published: January 2024

The emergence of antimicrobial resistance (AMR), particularly methicillin-resistant Staphylococcus aureus (MRSA), poses a significant global health threat as these bacteria increasingly become resistant to the most available therapeutic options. Thus, developing an efficient approach to rapidly screen MRSA directly from clinical specimens has become vital. In this study, we establish a closed-tube loop-mediated isothermal amplification (LAMP) method incorporating hydroxy-naphthol blue (HNB) colorimetric dye assay to directly detect MRSA from clinical samples based on the presence of mecA and spa genes. In total, 125 preidentified S. aureus isolates and 93 clinical samples containing S. aureus were sourced from the microbiology laboratory at Hamad General Hospital (HGH). The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were computed based on conventional PCR. The assay demonstrated 100% specificity, 91.23% sensitivity, 0.90 Cohen Kappa (CK), 100% PPV, and 87.8% NPV for the clinical samples, while clinical isolates exhibited 100% specificity, 97% sensitivity, 0.926 CK, 100% PPV, and 88.89% NPV. Compared to cefoxitin disk diffusion, LAMP provided 100% specificity and sensitivity, 1.00 CK, and 100% for PPV and NPV. The study revealed that the closed-tube LAMP incorporating (HNB) dye is a rapid technique with a turnaround time of less than 1 h and high specificity and sensitivity.
Correction: Mao et al. Gut Bacterial Community Determines the Therapeutic Effect of Ginsenoside on Canine Inflammatory Bowel Disease by Modulating the Colonic Mucosal Barrier. Microorganisms 2023, 11, 2616
Authors: Mao Aipeng , Zhao Weigang , Zhu Yuhang , Kong Fantao , Chen Danyang , Si Huazhe , Xu Chao
Published: January 2024

The authors wish to make the following corrections to this paper [...]
The Effect of Block Ratio and Structure on the Thermosensitivity of Double and Triple Betaine Block Copolymers
Authors: Lim Jongmin , Matsuoka Hideki , Kinoshita Yusuke , Yusa Shin-ichi , Saruwatari Yoshiyuki
Published: January 2024

AB-type and BAB-type betaine block copolymers composed of a carboxybetaine methacrylate and a sulfobetaine methacrylate, PGLBT-b-PSPE and PSPE-b-PGLBT-b-PSPE, respectively, were synthesized by one-pot RAFT polymerization. By optimizing the concentration of the monomer, initiator, and chain transfer agent, block extension with precise ratio control was enabled and a full conversion (~99%) of betaine monomers was achieved at each step. Two sets (total degree of polymerization: ~300 and ~600) of diblock copolymers having four different PGLBT:PSPE ratios were prepared to compare the influence of block ratio and molecular weight on the temperature-responsive behavior in aqueous solution. A turbidimetry and dynamic light scattering study revealed a shift to higher temperatures of the cloud point and micelle formation by increasing the ratio of PSPE, which exhibit upper critical solution temperature (UCST) behavior. PSPE-dominant diblocks created spherical micelles stabilized by PGLBT motifs, and the transition behavior diminished by decreasing the PSPE ratio. No particular change was found in the diblocks that had an identical AB ratio. This trend reappeared in the other set whose entire molecular weight approximately doubled, and each transition point was not recognizably impacted by the total molecular weight. For triblocks, the PSPE double ends provided a higher probability of interchain attractions and resulted in a more turbid solution at higher temperatures, compared to the diblocks which had similar block ratios and molecular weights. The intermediates assumed as network-like soft aggregates eventually rearranged to monodisperse flowerlike micelles. It is expected that the method for obtaining well-defined betaine block copolymers, as well as the relationship of the block ratio and the chain conformation to the temperature-responsive behavior, will be helpful for designing betaine-based polymeric applications.
Acoustic Emission Analysis of Mode II Interlaminar Fracture Toughness of 3D Reinforced CFRP
Authors: Oliveira Thiago Luiz Lara , Bortoluzzi Daniel Brighenti , Barbosa Lorena Cristina Miranda , Ancelotti Antônio Carlos
Published: January 2024

The use of composites in industry is increasing due to their ability to replace traditional materials. Carbon fiber-reinforced polymers offer a favorable strength-to-weight ratio, making them advantageous in numerous applications. Delamination is a common failure mode for composite materials, making it a crucial factor in ensuring material safety during service life. While fiber orientation in composites is designed for specific directional reinforcement, out-of-plane loads are often neglected, posing a critical challenge. Implementing through-thickness reinforcement, such as tufting, can enhance out-of-plane resistance, enabling more accurate structural designs. Non-destructive testing methods, particularly acoustic emission, play a significant role in ensuring component safety by detecting early damage and flaws. This study focused on monitoring mode II interlaminar fracture toughness and end-notched flexure (ENF), using acoustic emissions to compare the performance of samples with different through-thickness reinforcements against that of nonreinforced samples. The research analyzed acoustic emission patterns during testing, revealing a strong correlation with failure stages and the resistance induced by reinforcements. This approach provided valuable insights into damage characterization, supported by fractography analysis, especially concerning the final stages of failure due to damage, and the effects of different thread reinforcements. Acoustic emission proved crucial for real-time monitoring, enabling informed decisions to be made regarding component repair and lifespan extension in composite materials.
Extra-Axial Cavernous Angioma: A Case Report and Review of the Literature
Authors: Hassanzadeh Shakiba , Gao Linlin , Alvarado Anthony M. , Camarata Paul J. , Lakis Nelli S. , Haeri Mohammad
Published: January 2024

Cavernous angiomas (CAs) are benign vascular malformations predominantly seen in the brain parenchyma and therefore referred to as intra-axial. Extra-axial dural-based cavernous angiomas, on the other hand, are rare vascular lesions found outside of the brain parenchyma. They occur in the middle fossa and may be easily misdiagnosed as meningiomas due to their extra-axial location. In addition, CAs that are located outside the middle fossa, such as in the convexity, have a better prognosis since they are more surgically accessible. Surgical resection is the main treatment of choice in CAs. However, other options, such as embolization and radiotherapy, may also be considered therapeutic choices or additive treatment options. The pathogenesis of CA and the involvement of other factors (genetics or environmental factors) are still unknown and require further investigation. We are presenting a young man who presented for evaluation of seizure-like events without any family history of neurologic conditions. The physical examination was unremarkable except for a slightly antalgic gait. Imaging studies showed an extra-axial left tentorial mass suggestive of a meningioma, hemangiopericytoma, or other extra-axial lesions. The lesion was resected where its vascular nature was mentioned initially, and the histology proved the diagnosis of cavernous angioma. Here we give an overview of the known pathogenesis, causes, clinical features, and diagnostic and therapeutic options in CA. Better knowledge about CA, its causes, clinical features, and treatment options would help clinicians in early diagnosis and patient management.
Curcumin Mitigates the High-Fat High-Sugar Diet-Induced Impairment of Spatial Memory, Hepatic Metabolism, and the Alteration of the Gut Microbiome in Alzheimer’s Disease-Induced (3xTg-AD) Mice
Authors: Lamichhane Gopal , Liu Jing , Lee Su-Jeong , Lee Da-Yeon , Zhang Guolong , Kim Yoo
Published: January 2024

The escalating prevalence of metabolic diseases and an aging demographic has been correlated with a concerning rise in Alzheimer’s disease (AD) incidence. This study aimed to access the protective effects of curcumin, a bioactive flavonoid from turmeric, on spatial memory, metabolic functions, and the regulation of the gut microbiome in AD-induced (3xTg-AD) mice fed with either a normal chow diet (NCD) or a high-fat high-sugar diet (HFHSD). Our findings revealed an augmented susceptibility of the HFHSD-fed 3xTg-AD mice for weight gain and memory impairment, while curcumin supplementation demonstrated a protective effect against these changes. This was evidenced by significantly reduced body weight gain and improved behavioral and cognitive function in the curcumin-treated group. These improvements were substantiated by diminished fatty acid synthesis, altered cholesterol metabolism, and suppressed adipogenesis-related pathways in the liver, along with modified synaptic plasticity-related pathways in the brain. Moreover, curcumin enriched beneficial gut microbiota, including Oscillospiraceae and Rikenellaceae at the family level, and Oscillibacter, Alistipes, Pseudoflavonifractor, Duncaniella, and Flintibacter at the genus level. The observed alteration in these gut microbiota profiles suggests a potential crosswalk in the liver and brain for regulating metabolic and cognitive functions, particularly in the context of obesity-associated cognitive disfunction, notably AD.
Psychological Distress and Food Insecurity among International Students at a Hungarian University: A Post-Pandemic Survey
Authors: Hilal Soukaina , Kolozsvári László Róbert , Indrayathi Putu Ayu , Saeed Sami Najmaddin , Rurik Imre
Published: January 2024

The aim of the present study was two-fold: Firstly, to estimate the prevalence of psychological distress among international students at a Hungarian university two years after the COVID-19 outbreak; and secondly, to identify its demographic and socioeconomic factors, with special regard to the students’ food-security status. A cross-sectional study using a self-administered questionnaire was carried out from 27 March to 3 July 2022 among international students at the University of Debrecen. The questionnaire included information on demographic and socioeconomic characteristics, food-security status (six-item United States Department of Agriculture Food Security Survey Module (USDA-FSSM)), and psychological distress (Depression, Anxiety and Stress Scale (DASS-21)). Bivariate analysis was conducted to examine the potential associations between demographic/socioeconomic factors and psychological distress. Additionally, multiple logistic regression was employed to further analyze these associations. Of 398 participants, 42.2%, 48.7%, and 29.4% reported mild to extremely severe depression, anxiety, and stress, respectively. The ages 18–24 (AOR = 2.619; 95% CI: 1.206–5.689) and 25–29 (AOR = 2.663; 95% CI: 1.159–6.119), reporting a low perception of health status (AOR = 1.726; 95% CI: 1.081–2.755), and being food insecure (AOR = 1.984; 95% CI: 1.274–3.090) were significantly associated with depressive symptoms. Being female (AOR = 1.674; 95% CI: 1.090–2.571), reporting a low perception of health status (AOR = 1.736; 95% CI: 1.098–2.744), and being food insecure (AOR = 2.047; 95% CI: 1.327–3.157) were significantly associated with anxiety symptoms. Furthermore, being female (AOR = 1.702; 95% CI: 1.026–2.824)), living with roommates (AOR = 1.977; 95% CI: 1.075–3.635), reporting a low perception of health status (AOR = 2.840; 95% CI: 1.678–4.807), and being food insecure (AOR = 2.295; 95% CI:1.398–3.767) were significantly associated with symptoms of stress. Psychosocial programs combined with strategies to alleviate food insecurity are required to enhance international students’ mental health and well-being.
Associations between Serum Mineral Nutrients, Gut Microbiota, and Risk of Neurological, Psychiatric, and Metabolic Diseases: A Comprehensive Mendelian Randomization Study
Authors: Li Wang , Lv Bo-Min , Quan Yuan , Zhu Qiang , Zhang Hong-Yu
Published: January 2024

Recent observational studies have reported associations between serum mineral nutrient levels, gut microbiota composition, and neurological, psychiatric, and metabolic diseases. However, the causal effects of mineral nutrients on gut microbiota and their causal associations with diseases remain unclear and require further investigation. This study aimed to identify the associations between serum mineral nutrients, gut microbiota, and risk of neurological, psychiatric, and metabolic diseases using Mendelian randomization (MR). We conducted an MR study using the large-scale genome-wide association study (GWAS) summary statistics of 5 serum mineral nutrients, 196 gut microbes at the phylum, order, family, and genus levels, and a variety of common neurological, psychiatric, and metabolic diseases. Initially, the independent causal associations of mineral nutrients and gut microbiota with diseases were examined by MR. Subsequently, the causal effect of mineral nutrients on gut microbiota was estimated to investigate whether specific gut microbes mediated the association between mineral nutrients and diseases. Finally, we performed sensitivity analyses to assess the robustness of the study results. After correcting for multiple testing, we identified a total of 33 causal relationships among mineral nutrients, gut microbiota, and diseases. Specifically, we found 4 causal relationships between 3 mineral nutrition traits and 3 disease traits, 15 causal associations between 14 gut microbiota traits and 6 disease traits, and 14 causal associations involving 4 mineral nutrition traits and 15 gut microbiota traits. Meanwhile, 118 suggestive associations were identified. The current study reveals multiple causal associations between serum mineral nutrients, gut microbiota, risk of neurological, psychiatric, and metabolic diseases, and potentially provides valuable insights for subsequent nutritional therapies.
Citrus p-Synephrine Improves Energy Homeostasis by Regulating Amino Acid Metabolism in HFD-Induced Mice
Authors: Bai Junying , Tan Xiang , Tang Sheng , Liu Xin , Shao Linzi , Wang Chen , Huang Linhua
Published: January 2024

p-Synephrine is a common alkaloid widely distributed in citrus fruits. However, the effects of p-synephrine on the metabolic profiles of individuals with energy abnormalities are still unclear. In the study, we investigated the effect of p-synephrine on energy homeostasis and metabolic profiles using a high fat diet (HFD)-induced mouse model. We found that p-synephrine inhibited the gain in body weight, liver weight and white adipose tissues weight induced by HFD. p-Synephrine supplementation also reduced levels of serum total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) but not to a statistically significant degree. Histological analysis showed that HFD induced excessive lipid accumulation and glycogen loss in the liver and adipocyte enlargement in perirenal fat tissue, while p-synephrine supplementation reversed the changes induced by HFD. Moreover, HFD feeding significantly increased mRNA expression levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) and reduced the mRNA expression level of interleukin-10 (IL-10) compared to the control group, while p-synephrine supplementation significantly reversed these HFD-induced changes. Liver and serum metabolomic analysis showed that p-synephrine supplementation significantly altered small molecule metabolites in liver and serum in HFD mice and that the changes were closely associated with improvement of energy homeostasis. Notably, amino acid metabolism pathways, both in liver and serum samples, were significantly enriched. Our study suggests that p-synephrine improves energy homeostasis probably by regulating amino acid metabolism in HFD mice, which provides a novel insight into the action mechanism of p-synephrine modulating energy homeostasis.
Effects of Water Temperature and Structural Habitat Complexity on the Routine Swimming Speed and Escape Response of Post-Settlement Stage White Seabream
Authors: Vicente Patrícia , Almeida João , Ribeiro Laura , Castanho Sara , Candeias-Mendes Ana , Pousão-Ferreira Pedro , Faria Ana Margarida
Published: January 2024

Coastal habitats are increasingly threatened by multiple anthropogenic-related activities, which include ocean warming and loss of structural habitat complexity. These two pressures have the potential to severely affect the structure and function of marine biodiversity. Early life stages of many fish species recruit to coastal habitats at the end of their pelagic phase, benefiting from access to food, shelter and protection. However, changes in temperature have been shown to influence ecologically relevant behaviours in post-settlement stage fish, and the loss of structural habitat complexity has been related to low recruitment and deleterious behaviours of fish in coastal habitats. Here, we evaluated the individual and interactive effects of prolonged exposure to increasing temperature and changed structural habitat complexity on routine swimming speed and escape response of post-settlement white seabream, Diplodus sargus (Linnaeus, 1758). Fish were reared under different temperatures (control 19 °C; high 22 °C) and structural habitat complexity (low and high) scenarios, in a cross-experimental design, and the routine swimming and escape responses were analyzed after 6 weeks of exposure. Change in temperature did not induce alterations at the behavioural level, but loss of structural habitat complexity increased speed and distance travelled during routine swimming, and responsiveness to a stimulus during the escape response behaviour. The interaction of the two factors did not influence performance. Determining how species are affected by changes in their environment, and the mechanisms that underlie these changes, will be critical to understanding the fish recruitment and populations’ fitness and survival.
Effects of Age and Playing Position on Field-Based Physical Fitness Measures in Adolescent Female Netball Players
Authors: Hackett Daniel A. , Tran Derek L. , Way Kimberley L. , Sanders Ross H.
Published: January 2024

This cross-sectional study investigated the impact of age and playing position, controlling for maturity, on physical fitness indicators in 303 adolescent female netball players aged 12.0 to 15.9 years. Assessments included estimated maximal oxygen uptake (VO2max) via the 20 m shuttle run test, 10 m and 20 m sprints, change of direction speed (CODS) using the 505 test, and muscle power via the medicine ball chest throw (MBCT) and countermovement vertical jump (CMJ). Participants were grouped by age (12 to 15 years) and playing position (non-circle and circle players), with age at peak height velocity as a covariate for maturity. Results revealed that, at 15 years, CMJ height was greater than at 12 years and 13 years (p < 0.05, partial η2 = 0.048). MBCT distance increased across age groups (p < 0.01, partial η2 = 0.323). Age had no impact on sprints, VO2max, or CODS. Non-circle players outperformed circle players in the 10 m sprint (p = 0.042, partial η2 = 0.016) and 20 m sprints (p = 0.010, partial η2 = 0.025) and displayed higher VO2max (p < 0.001, partial η2 = 0.036). Circle players were taller (p = 0.046, partial η2 = 0.014) and heavier (p < 0.001, partial η2 = 0.040) than non-circle players. Playing positions showed no differences in CMJ and MBCT. In adolescent female netball players, only muscle power is influenced by age, while non-circle players exhibit superior aerobic fitness and speed compared to circle players. Coaches may be able to utilize the distinct age and playing position traits of adolescent netballers to inform player selection and design targeted training programs.
Preclinical Application of CEST MRI to Detect Early and Regional Tumor Response to Local Brain Tumor Treatment
Authors: Park Se-Weon , Lai Joseph H. C. , Han Xiongqi , Leung Vivian W. M. , Xiao Peng , Huang Jianpan , Chan Kannie W. Y.
Published: January 2024

Treating glioblastoma and monitoring treatment response non-invasively remain challenging. Here, we developed a robust approach using a drug-loaded liposomal hydrogel that is mechanically compatible with the brain, and, simultaneously, we successfully monitored early tumor response using Chemical Exchange Saturation Transfer (CEST) MRI. This CEST-detectable liposomal hydrogel was optimized based on a sustainable drug release and a soft hydrogel for the brain tumor, which is unfavorable for tumor cell proliferation. After injecting the hydrogel next to the tumor, three distinctive CEST contrasts enabled the monitoring of tumor response and drug release longitudinally at 3T. As a result, a continuous tumor volume decrease was observed in the treatment group along with a significant decrease in CEST contrasts relating to the tumor response at 3.5 ppm (Amide Proton Transfer; APT) and at −3.5 ppm (relayed Nuclear Overhauser Effect; rNOE) when compared to the control group (p < 0.05). Interestingly, the molecular change at 3.5 ppm on day 3 (p < 0.05) was found to be prior to the significant decrease in tumor volume on day 5. An APT signal also showed a strong correlation with the number of proliferating cells in the tumors. This demonstrated that APT detected a distinctive decrease in mobile proteins and peptides in tumors before the change in tumor morphology. Moreover, the APT signal showed a regional response to the treatment, associated with proliferating and apoptotic cells, which allowed an in-depth evaluation and prediction of the tumor treatment response. This newly developed liposomal hydrogel allows image-guided brain tumor treatment to address clinical needs using CEST MRI.
Chemical Camouflage Induced by Diet in a Pest Treehopper on Host Plants
Authors: Lima Luan Dias , Ceballos-González Amalia Victoria , Prato Amanda , Cavalleri Adriano , Trigo José Roberto , Nascimento Fábio Santos do
Published: January 2024

Ants patrol foliage and exert a strong selective pressure on herbivorous insects, being their primary predators. As ants are chemically oriented, some organisms that interact with them (myrmecophiles) use chemical strategies mediated by their cuticular hydrocarbons (CHCs) to deal with ants. Thus, a better understanding of the ecology and evolution of the mutualistic interactions between myrmecophiles and ants depends on the accurate recognition of these chemical strategies. Few studies have examined whether treehoppers may use an additional strategy called chemical camouflage to reduce ant aggression, and none considered highly polyphagous pest insects. We analyzed whether the chemical similarity of the CHC profiles of three host plants from three plant families (Fabaceae, Malvaceae, and Moraceae) and the facultative myrmecophilous honeydew-producing treehopper Aetalion reticulatum (Hemiptera: Aetalionidae), a pest of citrus plants, may play a role as a proximate mechanism serving as a protection against ant attacks on plants. We found a high similarity (>80%) between the CHCs of the treehoppers and two of their host plants. The treehoppers acquire CHCs through their diet, and the chemical similarity varies according to host plant. Chemical camouflage on host plants plays a role in the interaction of treehoppers with their ant mutualistic partners.
Crop Evolution of Foxtail Millet
Authors: Fukunaga Kenji , Kawase Makoto
Published: January 2024

Studies on the domestication, genetic differentiation, and crop evolution of foxtail millet are reviewed in this paper. Several genetic studies were carried out to elucidate the genetic relationships among foxtail millet accessions originating mainly from Eurasia based on intraspecific hybrid pollen semi-sterility, isozymes, DNA markers, and single-nucleotide polymorphisms. Most studies suggest that China is the center of diversity of foxtail millet, and landraces were categorized into geographical groups. These results indicate that this millet was domesticated in China and spread over Eurasia, but independent origin in other regions cannot be ruled out. Furthermore, the evolution of genes was reviewed (i.e., the Waxy gene conferring amylose content in the endosperm, the Si7PPO gene controlling polyphenol oxidase, the HD1 and SiPRR37 genes controlling heading time, the Sh1 and SvLes1 genes involved in grain shattering, and the C gene controlling leaf sheath pigmentation), and the variation and distribution of these genes suggested complex patterns of evolution under human and/or natural selection.
Genome-Wide Identification of bZIP Transcription Factors in Cymbidium ensifolium and Analysis of Their Expression under Low-Temperature Stress
Authors: Lai Huiping , Wang Mengyao , Yan Lu , Feng Caiyun , Tian Yang , Tian Xinyue , Peng Donghui , Lan Siren , Zhang Yanping , Ai Ye
Published: January 2024

The basic leucine zipper (bZIP) transcription factors constitute the most widely distributed and conserved eukaryotic family. They play crucial roles in plant growth, development, and responses to both biotic and abiotic stresses, exerting strong regulatory control over the expression of downstream genes. In this study, a genome-wide characterization of the CebZIP transcription factor family was conducted using bioinformatic analysis. Various aspects, including physicochemical properties, phylogenetics, conserved structural domains, gene structures, chromosomal distribution, gene covariance relationships, promoter cis-acting elements, and gene expression patterns, were thoroughly analyzed. A total of 70 CebZIP genes were identified from the C. ensifolium genome, and they were randomly distributed across 18 chromosomes. The phylogenetic tree clustered them into 11 subfamilies, each exhibiting complex gene structures and conserved motifs arranged in a specific order. Nineteen pairs of duplicated genes were identified among the 70 CebZIP genes, with sixteen pairs affected by purifying selection. Cis-acting elements analysis revealed a plethora of regulatory elements associated with stress response, plant hormones, and plant growth and development. Transcriptome and qRT-PCR results demonstrated that the expression of CebZIP genes was universally up-regulated under low temperature conditions. However, the expression patterns varied among different members. This study provides theoretical references for identifying key bZIP genes in C. ensifolium that confer resistance to low-temperature stress, and lays the groundwork for further research into their broader biological functions.
Laser-Driven Rapid Synthesis of Metal-Organic Frameworks and Investigation of UV-NIR Optical Absorption, Luminescence, Photocatalytic Degradation, and Gas and Ion Adsorption Properties
Authors: Mutlu Saliha , Ortaç Bülend , Ozbey Dogukan Hazar , Durgun Engin , Savaskan Yılmaz Sevil , Arsu Nergis
Published: January 2024

In this study, we designed a platform based on a laser-driven approach for fast, efficient, and controllable MOF synthesis. The laser irradiation method was performed for the first time to synthesize Zn-based MOFs in record production time (approximately one hour) compared to all known MOF production methods with comparable morphology. In addition to well-known structural properties, we revealed that the obtained ZnMOFs have a novel optical response, including photoluminescence behavior in the visible range with nanosecond relaxation time, which is also supported by first-principles calculations. Additionally, photocatalytic degradation of methylene blue with ZnMOF was achieved, degrading the 10 ppm methylene blue (MB) solution 83% during 1 min of irradiation time. The application of laser technology can inspire the development of a novel and competent platform for a fast MOF fabrication process and extend the possible applications of MOFs to miniaturized optoelectronic and photonic devices.
Investigation of Shape Memory Polyurethane Properties in Cold Programming Process Towards Its Applications
Authors: Staszczak Maria , Urbański Leszek , Cristea Mariana , Ionita Daniela , Pieczyska Elżbieta Alicja
Published: January 2024

Thermoresponsive shape memory polymers (SMPs) with the remarkable ability to remember a temporary shape and recover their original one using temperature have been gaining more and more attention in a wide range of applications. Traditionally, SMPs are investigated using a method named often “hot-programming”, since they are heated above their glass transition temperature (Tg) and after that, reshaped and cooled below Tg to achieve and fix the desired configuration. Upon reheating, these materials return to their original shape. However, the heating of SMPs above their Tg during a thermomechanical cycle to trigger a change in their shape creates a temperature gradient within the material structure and causes significant thermal expansion of the polymer sample resulting in a reduction in its shape recovery property. These phenomena, in turn, limit the application fields of SMPs, in which fast actuation, dimensional stability and low thermal expansion coefficient are crucial. This paper aims at a comprehensive experimental investigation of thermoplastic polyurethane shape memory polymer (PU-SMP) using the cold programming approach, in which the deformation of the SMP into the programmed shape is conducted at temperatures below Tg. The PU-SMP glass transition temperature equals approximately 65 °C. Structural, mechanical and thermomechanical characterization was performed, and the results on the identification of functional properties of PU-SMPs in quite a large strain range beyond yield limit were obtained. The average shape fixity ratio of the PU-SMP at room temperature programming was found to be approximately 90%, while the average shape fixity ratio at 45 °C (Tg − 20 °C) was approximately 97%. Whereas, the average shape recovery ratio was 93% at room temperature programming and it was equal to approximately 90% at 45 °C. However, the results obtained using the traditional method, the so-called hot programming at 65 °C, indicate a higher shape fixity value of 98%, but a lower shape recovery of 90%. Thus, the obtained results confirmed good shape memory properties of the PU-SMPs at a large strain range at various temperatures. Furthermore, the experiments conducted at both temperatures below Tg demonstrated that cold programming can be successfully applied to PU-SMPs with a relatively high Tg. Knowledge of the PU-SMP shape memory and shape fixity properties, estimated without risk of material degradation, caused by heating above Tg, makes them attractive for various applications, e.g., in electronic components, aircraft or aerospace structures.
Enhancing Paper Packaging’s Wet Strength Using the Synergy between Chitosan and Nanofibrillated Cellulose Additives
Authors: Andze Laura , Skute Marite , Zoldners Juris , Andzs Martins , Sirmulis Gatis , Irbe Ilze , Milbreta Ulla , Dabolina Inga , Filipova Inese
Published: January 2024

The demand for eco-friendly packaging materials has urged researchers to look for alternatives to petroleum-based polymers. In this regard, paper-based products have turned out to be a promising choice; however, their weak resistance to water has limited their application. The use of various additives to enhance paper’s moisture resistance is a common practice. However, considering the growing global agenda for sustainable development, the search for new bio-based paper additives has become increasingly important. This study investigated the potential synergistic impact of the addition of nanofibrillated cellulose (NFC) and chitosan additives (CHIT) to different fiber combinations to improve paper’s properties, in particular, their wet strength. The efficacy of the additive application order was examined and was found to be crucial in achieving the desired outcomes. The results showed that incorporating CHIT after NFC enhanced the paper’s tensile and burst indicators, as well as the paper stretch in the dry state, by 35–70%, 35–55%, and 20–35%, respectively. In addition, the tensile index and stretch in the wet state improved 9–13 times and 2.5–5.5 times over, respectively. The air permeability decreased 2.5–12 times over. These findings demonstrate that the sequential addition of the NFC and CHIT additives yield a greater enhancement of paper’s properties than using each additive separately.
Geographical Information System Modeling for Planning Internal Transportation in a Manufacturing Plant’s Outdoor Area
Authors: Prah Klemen , Gajšek Brigita
Published: January 2024

A geographical information system (GIS) is an advanced tool for collecting, managing, and analyzing spatially-referenced data. The contribution of GIS use to process performance indicators can be improved by combining it with multi-criteria decision analysis (MCDA). Combining a GIS and MCDA is, in the scientific literature, rarely discussed for planning an internal transportation system in a manufacturing plant’s outdoor area. The purpose of this article is to clarify what mangers can expect from using a combined approach when deciding on a transport fleet and the operational routing of vehicles. Beside the simulation of MCDA, the computer software ArcGIS Pro 3.0.2 with the Network Analyst extension was used for modelling the transportation system in the form of a case study. The article demonstrates the feasibility and effectiveness of GIS and MCDA use and reveals the extent of the challenge of how decision makers could make the most of ArcGIS functionality. The final solution for an internal transportation system in a manufacturing plant’s outdoor area includes such a vehicle fleet and the set time windows of orders for transport services, so that there are no violations of time windows and the work is completed within the work shift while minimizing costs, time, and distance. Decision makers can use the program without advanced knowledge of optimization approaches, following a procedure that does not differ much from that of learning to use other business software tools. On the contrary, the listed disadvantages can be summarized as the rigidity of setting detailed boundary conditions for a specific simulation scenario.
The Question of Beauty and the Aesthetic Value of the Image of the Mother of God in Pastoral Care and Catechesis
Authors: Pevec Rozman Mateja , Strehovec Tadej
Published: January 2024

Ancient philosophers attached great importance to the ideals of unity, truth, goodness, and beauty as the path to the greatest good. Beauty expressed through works of art can open the eyes of the mind and heart and direct the human spirit to transcendence. The beauty of art awakens inner emotionality, evokes elation in silence, and leads to “coming out of oneself”. The concept of beauty is inextricably linked to Catholic theology and preaching. Beautiful sacred images were a source of theological messages, intercession, and entry into the transcendental world. Medieval Gothic cathedrals had images on the walls as a basic tool of catechesis. Even in today’s teaching of young people, images play a crucial role. The world of symbols enables Christians to connect everyday life experiences with theological messages. The image of the Virgin Mary is the best example of recognizing personal life situations, with the story of a mother who loved her child, accepted the suffering and death of her own son, and together with St. Joseph formed a holy family, which is the image of an imperfect family in which each person recognizes himself. All these aspects of the life of the Virgin Mary could form the basic concepts of the Christian understanding of beauty. In modern thought, the concept of beauty is understood quite narrowly (we are talking about narrowing the meaning of the concept of beauty). In the first part of this paper, we focus on the philosophical concept of beauty with a brief historical overview, then we point out the difference between transcendental beauty and aesthetic beauty. Beauty appeals to the human being and opens the heart to the transcendent, to God, who is the source and fullness of beauty, beauty itself. The originality of the article is in its presentation of the understanding of the Christian concept of beauty through the figure and image of Mary, the Mother of God. The experience of the beauty of Mary and Mary’s life story enables the believer to have a different perspective on the perception of his own life and thereby opens him to the transcendent, to a personal relationship with God, who is eternal Beauty.
Evaluation of Sentinel-2 Based Chlorophyll-a Estimation in a Small-Scale Reservoir: Assessing Accuracy and Availability
Authors: Jang Wonjin , Kim Jinuk , Kim Jin Hwi , Shin Jae-Ki , Chon Kangmin , Kang Eue Tae , Park Yongeun , Kim Seongjoon
Published: January 2024

Small-scale reservoirs located in river estuaries are a significant water resource supporting agricultural and industrial activities; however, they face annual challenges of eutrophication and algal bloom occurrences due to excessive nutrient accumulation and watershed characteristics. Efficient management of algal blooms necessitates a comprehensive analysis of their spatiotemporal distribution characteristics. Therefore, this study aims to develop a chlorophyll-a (Chl-a) estimation model based on high-resolution satellite remote sensing data from Sentinel-2 multispectral sensors and multiple linear regression. The multiple linear regression (MLR) models were constructed using multiple reflectance-based variables that were collected over 2 years (2021–2022) in an estuarine reservoir. A total of 21 significant input variables were selected by backward elimination from the 2–4 band algorithms as employed in previous Chl-a estimation studies, along with the Sentinel-2 B1-B8A wavelength ratio. The developed algorithm exhibited a coefficient of determination of 0.65. Spatiotemporal variations in Chl-a concentration generated by the algorithm reflected the movement of high Chl-a concentration zones within the body of water. Through this analysis, it turned out that Sentinel-2-based spectral images were applicable to a small-scale reservoir which is relatively long and narrow, and the algorithm estimated changes in concentration levels over the seasons, revealing the dynamic nature of Chl-a distributions. The model developed in this study is expected to support effective algal bloom management and water quality improvement in a small-scale reservoir or similar complex water quality water bodies.
Analysis of Ozone Formation Sensitivity in Chinese Representative Regions Using Satellite and Ground-Based Data
Authors: Li Yichen , Yu Chao , Tao Jinhua , Lu Xiaoyan , Chen Liangfu
Published: January 2024

O3 poses a significant threat to human health and the ecological environment. In recent years, O3 pollution has become increasingly serious, making it difficult to accurately control O3 precursor emissions. Satellite indicator methods, such as the FNR (formaldehyde-to-nitrogen dioxide ratio (HCHO/NO2 ratio)), provide an effective way to identify ozone pollution control areas on a large geographical scale due to their simple acquisition of datasets. This can help determine the primary factors contributing to O3 pollution and assist in managing it. Based on TROPOMI data from May 2018 to December 2022, combined with ground-based monitoring data from the China National Environmental Monitoring Centre, we explored the uncertainty associated with using the HCHO/NO2 ratio (FNR) as an indicator in ozone control area determination. We focused on the four representative regions in China: Jing-Jin-Ji-Lu-Yu (JJJLY), Jiang-Zhe-Hu-Wan (JZHW), Chuan-Yu (CY), and South China. By using the statistical curve-fitting method, we found that the FNR thresholds were 3.5–5.1, 2.0–4.0, 2.5–4.2, and 1.7–3.5, respectively. Meanwhile, we analyzed the spatial and temporal characteristics of the HCHO, NO2, and O3 control areas. The HCHO concentrations and NO2 concentrations had obvious cyclical patterns, with higher HCHO column densities occurring in summer and higher NO2 concentrations in winter. These high values always appeared in areas with dense population activities and well-developed economies. The distribution characteristics of the ozone control areas indicated that during O3 pollution periods, the urban areas with industrial activities and high population densities were primarily controlled by VOCs, and the suburban areas gradually shifted from VOC-limited regimes to transitional regimes and eventually reverted back to VOC-limited regimes. In contrast, the rural and other remote areas with relatively less development were mainly controlled by NOx. The FNR also exhibited periodic variations, with higher values mostly appearing in summer and lower values appearing in winter. This study identifies the main factors contributing to O3 pollution in different regions of China and can serve as a valuable reference for O3 pollution control.