bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of an Anti-Thrombotic Continuous Lactate and Blood Pressure Monitoring Catheter in an In Vivo Piglet Model undergoing Open-Heart Surgery with Cardiopulmonary Bypass
Authors: Ho Kenneth Kwun Yin , Peng Yun-Wen , Ye Minyi , Tchouta Lise , Schneider Bailey , Hayes McKenzie , Toomasian John , Cornell Marie , Rojas-Pena Alvaro , Charpie John , Chen Hao
Published: July 2020

Blood lactate and blood pressure measurements are important predictors of life-threatening complications after infant open-heart surgeries requiring cardiopulmonary bypass (CPB). We have developed an intravascular nitric oxide (NO)-releasing 5-Fr catheter that contains a lactate sensor for continuous in-blood lactate monitoring and a dedicated lumen for third-party pressure sensor attachment. This device has antimicrobial and antithrombotic properties and can be implanted intravascularly. The importance of this design is its ability to inhibit thrombosis, due to the slow release of NO through the surface of the catheter and around the electrochemical lactate sensors, to allow continuous data acquisition for more than 48 h. An in vivo study was performed using six piglets undergoing open-heart surgery with CPB and cardioplegic arrest, in order to mimic intra-operative conditions for infants undergoing cardiac surgery with CPB. In each study of 3 h, two 5-Fr NO-releasing lactate and blood-pressure monitoring catheters were implanted in the femoral vessels (arteries and veins) and the CPB circuitry to monitor changing lactate levels and blood pressures during and immediately after aortic cross-clamp removal and separation from CBP. Electrical signals continuously acquired through the sensors were processed and displayed on the device’s display and via Bluetooth to a computer in real-time with the use of a two-point in vivo calibration against blood gas results. The study results show that lactate levels measured from those sensors implanted in the CPB circuit during CPB were comparable to those acquired by arterial blood gas measurements, whereas lactate levels measured from sensors implanted in the femoral artery were closely correlated with those acquired intermittently by blood gas prior to CPB initiation, but not during CPB. Blood pressure sensors attached to one lumen of the device displayed accurate blood pressure readings compared to those measured using an FDA approved pressure sensor already on the market. We recommend that the sensor be implanted in the CPB’s circuit to continuously monitor lactate during CPB, and implanted in the femoral arteries or jugular veins to monitor lactate before and after CPB. Blood pressures dramatically drop during CPB due to lower blood flow into the lower body, and we suspect that the femoral arteries are likely collapsing or constricting on the implanted catheter and disrupting the sensor-to-blood contact. This study shows that the device is able to accurately and continuously monitor lactate levels during CPB and potentially prevent post-surgery complications in infants.
Microstructural Characteristics in Babbitt Coatings Deposited by LPCS
Authors: Tillmann Wolfgang , Hagen Leif , Abdulgader Mohamed , Kensy Mark Dennis , Paulus Michael
Published: July 2020

Studies have already established that the mechanical properties of Babbitt coatings significantly depend on the microstructural characteristics, such as the amount and distribution of intermetallic compounds dispersed in a soft solid solution matrix. For Sn–Sb–Cu-based Babbitt coatings, the formation of SbSn- and CuSn-based precipitates has a substantial influence on the resulting microhardness and thus determines the maximum load carrying capacity. Thermal spraying of Sn-based Babbitt coatings results in a relatively more refined structure of these precipitates than in common manufacturing processes, such as casting, due to the thermal processing conditions. This study aims to evaluate the effect of the temperature of the propellant gas and substrate temperature on the microstructural characteristics of Sn–Sb–Cu-based Babbitt coatings deposited by low pressure cold spraying (LPCS). The deposits were examined for their phase composition, microhardness and mesoscopic structure. It was found that the coatings were mainly composed of Sb2Sn23, Sb0.49Sn0.51 and Sorosite (CuSn or CuSb0.115Sn0.835), regardless of the substrate temperature or temperature of the propellant gas to be investigated. For a gas temperature above 300 °C, an increased microhardness was observed, which correlates with the appearance of a more homogenous distribution of Sb0.49Sn0.51 dispersed in a soft Sn-rich solid solution matrix.
Conserving the Genetic Diversity of Domesticated Livestock
Authors: Sponenberg D. Phillip
Published: July 2020

Domesticated animals live and produce in an environment influenced by both natural and human factors. These agricultural environments are important to maintain for human survival and also for their interactions with natural environments. Effective conservation of domesticated biodiversity can help to assure sustainable agricultural systems that minimize negative influences on natural environments. In addition, livestock biodiversity is a component of total biodiversity and for several species is the only remaining source of diversity because the wild ancestors are now extinct. Conservation of livestock biodiversity depends on cultural and biological approaches. Each of these has differential importance depending on the specific location of the genetic resource as well as the human culture in which it resides. Effective global conservation blends these in different measures to assure positive outcomes that succeed in securing the genetic resource as well as its contribution to human survival and well-being.
Pseudo-Yang-Lee Edge Singularity Critical Behavior in a Non-Hermitian Ising Model
Authors: Zhai Liang-Jun , Huang Guang-Yao , Wang Huai-Yu
Published: July 2020

The quantum phase transition of a one-dimensional transverse field Ising model in an imaginary longitudinal field is studied. A new order parameter M is introduced to describe the critical behaviors in the Yang-Lee edge singularity (YLES). The M does not diverge at the YLES point, a behavior different from other usual parameters. We term this unusual critical behavior around YLES as the pseudo-YLES. To investigate the static and driven dynamics of M, the (1+1) dimensional ferromagnetic-paramagnetic phase transition ((1+1) D FPPT) critical region, (0+1) D YLES critical region and the (1+1) D YLES critical region of the model are selected. Our numerical study shows that the (1+1) D FPPT scaling theory, the (0+1) D YLES scaling theory and (1+1) D YLES scaling theory are applicable to describe the critical behaviors of M, demonstrating that M could be a good indicator to detect the phase transition around YLES. Since M has finite value around YLES, it is expected that M could be quantitatively measured in experiments.
Influence of Two-Phase Crossflow for Void Prediction in Bundles Using Thermal-Hydraulic System Codes
Authors: Lee Yunseok , Kim Taewan
Published: July 2020

The previous study, where the void fraction predictability of three different thermal-hydraulic system codes was assessed against PSBT (PWR Subchannel and Bundle Test) benchmark data, indicated a general overprediction tendency of all system codes, especially in bundles. Because all codes have been utilized for best-estimate analyses, it is necessary to conduct further assessments in order to find the root cause of the overprediction. A further assessment has been performed using two thermal-hydraulic system codes, TRACE V5.0 patch 5 and MARS-KS 1.4, and the assessment has been carried out for both one- and multi-dimensional components. The results indicate that there is no significant difference in the predictability of the void fraction between one- and multi-dimensional components. In addition, it is found that the turbulent mixing model implemented for the multi-dimensional component of MARS-KS does not play an important role in the prediction of void distribution. Meanwhile, TRACE reveals a significant overprediction due to much less crossflow calculation compared to MARS-KS. By conducting an additional analysis with the modified one-dimensional models, it is clearly confirmed that crossflow significantly affects the void distribution. Therefore, it is concluded that the model for the thermal hydraulic mixing by crossflow in each system code should be improved in order to predict the void distribution in bundles appropriately.
Smart Sanitation—Biosensors as a Public Health Tool in Sanitation Infrastructure
Authors: Rary Emma , Anderson Sarah M. , Philbrick Brandon D. , Suresh Tanvi , Burton Jasmine
Published: July 2020

The health of individuals and communities is more interconnected than ever, and emergent technologies have the potential to improve public health monitoring at both the community and individual level. A systematic literature review of peer-reviewed and gray literature from 2000-present was conducted on the use of biosensors in sanitation infrastructure (such as toilets, sewage pipes and septic tanks) to assess individual and population health. 21 relevant papers were identified using PubMed, Embase, Global Health, CDC Stacks and NexisUni databases and a reflexive thematic analysis was conducted. Biosensors are being developed for a range of uses including monitoring illicit drug usage in communities, screening for viruses and diagnosing conditions such as diabetes. Most studies were nonrandomized, small-scale pilot or lab studies. Of the sanitation-related biosensors found in the literature, 11 gathered population-level data, seven provided real-time continuous data and 14 were noted to be more cost-effective than traditional surveillance methods. The most commonly discussed strength of these technologies was their ability to conduct rapid, on-site analysis. The findings demonstrate the potential of this emerging technology and the concept of Smart Sanitation to enhance health monitoring at the individual level (for diagnostics) as well as at the community level (for disease surveillance).
Physical Self-Concept, Gender, and Physical Condition of Bizkaia University Students
Authors: Sáez Iker , Solabarrieta Josu , Rubio Isabel
Published: July 2020

(1) Background: Despite the benefits of physical activity being well documented in university students, some do not follow the international recommendations. This period of life is a vital stage in adhering to healthy habits in adult life. The objective of the study was to analyze university students’ scores of their physical self-concept and its relationship with gender, physical condition, and level of self-perceived competence. (2) Methods: The sample comprised of 1289 Bizkaia University students (42.12% men and 57.87% women), between 18 and 46.5 years old (M = 20.4; SD = 2.2 years). Physical self-concept, physical condition, number of hours per week of physical activity, and perceived fitness level were analyzed. (3) Results: We found significant differences between women and men in their physical self-concept, but it seemed to be mainly an indirect effect through the mediation of hours of exercise and physical condition. (4) Conclusions: To understand the variance of the level of physical self-concept between genders in university students, the effect of certain variables (physical condition, number of hours per week, and perceived fitness level) must be considered, as well as the mediating role of some of these variables.
Sex Differences in Maintaining the Requested Handgrip Force Enhanced by Auditory or Visual Feedback
Authors: Tarnas Jacek , Stemplewski Rafał , Krutki Piotr
Published: July 2020

Thus far, the differences in effect of auditory or visual feedback in motor learning have presented results derived from mixed groups and sex differences have not been considered. However, perception and processing of auditory stimuli and performance of visual motor tasks appear to be sex-related. The purpose of this study was to investigate the learning of the simple motor task of maintaining a requested handgrip force in separate male and female groups. A total of 31 volunteers (15 males, 16 females) were randomly assigned to one of four experimental groups with defined sex and training conditions (audio or visual feedback). Participants performed training sessions over a period of six days, for which auditory or visual feedback was provided, and the effectiveness of both types of signals was compared. The evident learning effect was found in all groups, and the main effect of sex was significant among visual groups in favor of the males (p < 0.05). On the other hand, the main effect of feedback conditions was found to be significant among females, beneficially in the case of auditory displays (p < 0.05). The results lead to the conclusion that an equal number of males and females in mixed experimental groups may be supportive to obtain reliable results. Moreover, in motor-learning studies conducted on females only, a design including auditory feedback would be more suitable.
Working towards a Co-Ordinated Approach to Invasive Mosquito Detection, Response and Control in the UK
Authors: Vaux Alexander G. C. , Johnston Colin , Dallimore Thom , McGinley Liz , Strode Clare , Murchie Archie K. , Iyanger Nalini , Pudney Rachel , Chow Yimmy , Brand Martin , Rea Ian , Medlock Jolyon M.
Published: July 2020

The United Kingdom (UK) has reported a single detection of the eggs of the invasive mosquito vector Aedes albopictus in each of the three years from 2016 to 2018, all in southeast England. Here, we report the detection of mosquito eggs on three occasions at two sites in London and southeast England in September 2019. Mosquito traps were deployed at 56 sites, in England, Scotland, Wales, and Northern Ireland, as part of a coordinated surveillance programme with local authorities, Edge Hill University, and government departments. Response to each detection was coordinated by Public Health England’s (PHE) local health protection teams, with technical support from PHE’s Medical Entomology group, and control conducted by the respective local authority. Control, including source reduction and larviciding, was conducted within a 300 metre radius of the positive site. The response followed a National Contingency Plan for Invasive Mosquitoes: Detection of Incursions. Although the response to these incidents was rapid and well co-ordinated, recommendations are made to further develop mosquito surveillance and control capability for the UK.
Anti-Inflammatory and Antioxidative Potential of Aloe vera on the Cartap and Malathion Mediated Toxicity in Wistar Rats
Authors: Gupta Vivek Kumar , Kumar Abhishek , Pereira Maria de Lourdes , Siddiqi Nikhat Jamal , Sharma Bechan
Published: July 2020

Aloe vera has been the most useful medicinal herb in the world since ancient times due to its vast biological effects. The presence of high content of bioactive compounds make Aloe vera a promising complementary and alternative agent in disease prevention. The effectiveness of A. vera-based medicines against pesticide toxicity has never been evaluated. It was therefore envisaged to develop an A. vera-based strategy to protect the non-target animals from adverse effects of the pesticides. This article illustrates the ameliorating effect of aqueous extract (AE) of A. vera leaves against the cartap and malathion toxicity. To evaluate the protective impact of A. vera against cartap (Ctp), malathion (Mtn) and a mixture of both pesticides, the animals were divided in eight groups, each containing six rats: Group 1- C (control), Group 2- AE + C, Group 3- Ctp, Group 4- Mtn, Group 5- Ctp + Mtn, Group 6- AE + Ctp, Group 7- AE + Mtn, Group 8- AE + Ctp + Mtn. Wistar rats exposed to Ctp, Mtn and Ctp + Mtn, displayed significant change in body weight. It was observed that the WBC level increased significantly in Mtn and Ctp + Mtn challenged groups. The contents of TNF-α and IL-6 in serum increased expressively in the Ctp, Mtn and Ctp + Mtn challenged groups. Rats treated with Ctp, Mtn and Ctp + Mtn displayed significant alterations in the levels of antioxidative indices (MDA, GSH, GST, GPx, SOD and CAT). Significant alterations were recorded in the activities of AST, ALT, ACP and ALP in Ctp, Mtn and Ctp + Mtn challenged groups. The histopathological results of liver supported the biochemical data. The pre-treatment of rats with the aqueous extract of A. vera leaves significantly protected them from the toxicity of pesticides. These results suggested that A. vera extract may be used as a promising natural agent for the management of pesticide induced toxicity.
How Postural Muscle Senses Disuse? Early Signs and Signals
Authors: Shenkman Boris S.
Published: July 2020

A mammalian soleus muscle along with other “axial” muscles ensures the stability of the body under the Earth’s gravity. In rat experiments with hindlimb suspension, zero-gravity parabolic flights as well as in human dry immersion studies, a dramatic decrease in the electromyographic (EMG) activity of the soleus muscle has been repeatedly shown. Most of the motor units of the soleus muscle convert from a state of activity to a state of rest which is longer than under natural conditions. And the state of rest gradually converts to the state of disuse. This review addresses a number of metabolic events that characterize the earliest stage of the cessation of the soleus muscle contractile activity. One to three days of mechanical unloading are accompanied by energy-dependent dephosphorylation of AMPK, accumulation of the reactive oxygen species, as well as accumulation of resting myoplasmic calcium. In this transition period, a rapid rearrangement of the various signaling pathways occurs, which, primarily, results in a decrease in the rate of protein synthesis (primarily via inhibition of ribosomal biogenesis and activation of endogenous inhibitors of mRNA translation, such as GSK3β) and an increase in proteolysis (via upregulation of muscle-specific E3-ubiquitin ligases).
Transglutaminase 2-Mediated p53 Depletion Promotes Angiogenesis by Increasing HIF-1α-p300 Binding in Renal Cell Carcinoma
Authors: Lee Seon-Hyeong , Kang Joon Hee , Ha Ji Sun , Lee Jae-Seon , Oh Su-Jin , Choi Hyun-Jung , Song Jaewhan , Kim Soo-Youl
Published: July 2020

Angiogenesis and the expression of vascular endothelial growth factor (VEGF) are increased in renal cell carcinoma (RCC). Transglutaminase 2 (TGase 2), which promotes angiogenesis in endothelial cells during wound healing, is upregulated in RCC. Tumor angiogenesis involves three domains: cancer cells, the extracellular matrix, and endothelial cells. TGase 2 stabilizes VEGF in the extracellular matrix and promotes VEGFR-2 nuclear translocation in endothelial cells. However, the role of TGase 2 in angiogenesis in the cancer cell domain remains unclear. Hypoxia-inducible factor (HIF)-1α-mediated VEGF production underlies the induction of angiogenesis in cancer cells. In this study, we show that p53 downregulated HIF-1α in RCC, and p53 overexpression decreased VEGF production. Increased TGase 2 promoted angiogenesis by inducing p53 degradation, leading to the activation of HIF-1α. The interaction of HIF-1α and p53 with the cofactor p300 is required for stable transcriptional activation. We found that TGase 2-mediated p53 depletion increased the availability of p300 for HIF-1α-p300 binding. A preclinical xenograft model suggested that TGase 2 inhibition can reverse angiogenesis in RCC.
How Thick Is the Oral Mucosa around Implants after Augmentation with Different Materials: A Systematic Review of the Effectiveness of Substitute Matrices in Comparison to Connective Tissue Grafts
Authors: Lissek Martin , Boeker Martin , Happe Arndt
Published: July 2020

This systematic review aimed to assess the effectiveness of xenogeneic collagen matrices (XCMs) and acellular dermal matrices (ADMs) in comparison to connective tissue grafts (CTGs) for the augmentation of oral mucosa around dental implants. MEDLINE and the Web of Science were searched for clinical studies that compared substitute materials for the augmentation of oral mucosa to the subepithelial connective tissue graft around dental implants during or after implantation. The review was conducted according to the recommendations of the PRISMA statement. From an initial search result set of 1050 references, seven articles were included in the review. The study designs were heterogeneous, so no meta-analysis could be performed. Both the CTG and either type of substitute material resulted in increased mucosal thickness. Four studies showed no significant difference, while three demonstrated a significant difference, favoring the CTGs over alternative materials. Soft tissue augmentation around dental implants is a safe procedure and leads to thicker mucosal tissue. The subepithelial connective tissue graft can still be regarded as the gold standard, but substitute materials may be an acceptable alternative in some situations, such as for pain-sensitive patients, among inexperienced surgeons, and for sites with an already thick biotype.
Proteomic Analysis in Seminal Plasma of Fertile Donors and Infertile Patients with Sperm DNA Fragmentation
Authors: Fernandez-Encinas Alba , García-Peiró Agustín , del Rey Javier , Ribas-Maynou Jordi , Abad Carlos , Amengual Maria José , Prada Elena , Navarro Joaquima , Benet Jordi
Published: July 2020

Seminal plasma proteomics studies could represent a new approach for the determination of molecular elements driving male infertility, resulting in a better male infertility characterization. The aim of this study is to investigate proteomic differences in seminal plasma samples from fertile and infertile individuals. For that, semen samples were selected according to semen analysis, clinical pathology, and values of sperm DNA fragmentation (alkaline and neutral Comet assay and Sperm Chromatin Dispersion test). A total of 24 seminal plasma samples classified in four groups were processed: fertile donors (FD), recurrent miscarriage patients (RM), asthenoteratozoospermic patients (ATZ), and asthenoteratozoospermic patients with varicocele (ATZ-VAR). Results obtained by 2D-differential gel electrophoresis (2D-DIGE) revealed 26 spots significantly increased in fertile donors when compared to patient groups. Also, eight spots in the ATZ group and two in the ATZ-VAR group were decreased compared to the other groups. Twenty-eight proteins were identified by mass spectrometry (MS), most of them involved in metabolic and cellular processes and with a catalytic or binding function. Protein–protein interactions through Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) tool suggest that a large part of them were associated with each other. Furthermore, most of them were associated with ubiquitin C, indicating that it could play an important regulation role, resulting in a potential male infertility biomarker.
Cardiomyopathies and Adrenal Diseases
Authors: Petramala Luigi , Concistrè Antonio , Olmati Federica , Saracino Vincenza , Chimenti Cristina , Frustaci Andrea , Russo Matteo A. , Letizia Claudio
Published: July 2020

Cardiomyopathies are myocardial disorders in which heart muscle is structurally and/or functionally abnormal. Previously, structural cardiomyocyte disorders due to adrenal diseases, such as hyperaldosteronism, hypercortisolism, and hypercatecholaminism, were misunderstood, and endomyocardial biopsy (EMB) was not performed because was considered dangerous and too invasive. Recent data confirm that, if performed in experienced centers, EMB is a safe technique and gives precious information about physiopathological processes implied in clinical abnormalities in patients with different systemic disturbances. In this review, we illustrate the most important features in patients affected by primary aldosteronism (PA), Cushing’s syndrome (CS), and pheochromocytoma (PHEO). Then, we critically describe microscopic and ultrastructural aspects that have emerged from the newest EMB studies. In PA, the autonomous hypersecretion of aldosterone induces the alteration of ion and water homeostasis, intracellular vacuolization, and swelling; interstitial oedema could be a peculiar feature of myocardial toxicity. In CS, cardiomyocyte hypertrophy and myofibrillolysis could be related to higher expression of atrogin-1. Finally, in PHEO, the hypercontraction of myofilaments with the formation of contraction bands and occasional cellular necrosis has been observed. We expect to clear the role of EMB in patients with cardiomyopathies and adrenal disease, and we believe EMB is a valid tool to implement new management and therapies.
Gemfibrozil Induces Anemia, Leukopenia and Reduces Hematopoietic Stem Cells via PPAR-α in Mice
Authors: Estrela Gabriel Rufino , Arruda Adriano Cleis , Torquato Heron Fernandes Vieira , Freitas-Lima Leandro Ceotto , Perilhão Mauro Sérgio , Wasinski Frederick , Budu Alexandre , Fock Ricardo Ambrósio , Paredes-Gamero Edgar Julian , Araujo Ronaldo Carvalho
Published: July 2020

Hypercholesterolemia, also called high cholesterol, is a form of hyperlipidemia, which may be a consequence of diet, obesity or diabetes. In addition, increased levels of low-density lipoprotein (LDL) and reduced levels of high-density lipoprotein (HDL) cholesterol are associated with a higher risk of atherosclerosis and coronary heart disease. Thus, controlling cholesterol levels is commonly necessary, and fibrates have been used as lipid-lowering drugs. Gemfibrozil is a fibrate that acts via peroxisome proliferator-activated receptor alpha to promote changes in lipid metabolism and decrease serum triglyceride levels. However, anemia and leukopenia are known side effects of gemfibrozil. Considering that gemfibrozil may lead to anemia and that gemfibrozil acts via peroxisome proliferator-activated receptor alpha, we treated wild-type and peroxisome proliferator-activated receptor alpha-knockout mice with gemfibrozil for four consecutive days. Gemfibrozil treatment led to anemia seven days after the first administration of the drug; we found reduced levels of hemoglobin, as well as red blood cells, white blood cells and a reduced percentage of hematocrits. PPAR-alpha-knockout mice were capable of reversing all of those reduced parameters induced by gemfibrozil treatment. Erythropoietin levels were increased in the serum of gemfibrozil-treated animals, and we also observed an increased expression of hypoxia-inducible factor-2 alpha (HIF-2α) and erythropoietin in renal tissue, while PPAR-alpha knockout mice treated with gemfibrozil did not present increased levels of serum erythropoietin or tissue HIF-2α and erythropoietin mRNA levels in the kidneys. We analyzed bone marrow and found that gemfibrozil reduced erythrocytes and hematopoietic stem cells in wild-type mice but not in PPAR-alpha-knockout mice, while increased colony-forming units were observed only in wild-type mice treated with gemfibrozil. Here, we show for the first time that gemfibrozil treatment leads to anemia and leukopenia via peroxisome proliferator-activated receptor alpha in mice.
Podocytes—The Most Vulnerable Renal Cells in Preeclampsia
Authors: Kwiatkowska Ewa , Stefańska Katarzyna , Zieliński Maciej , Sakowska Justyna , Jankowiak Martyna , Trzonkowski Piotr , Marek-Trzonkowska Natalia , Kwiatkowski Sebastian
Published: July 2020

Preeclampsia (PE) is a disorder that affects 3–5% of normal pregnancies. It was believed for a long time that the kidney, similarly to all vessels in the whole system, only sustained endothelial damage. The current knowledge gives rise to a presumption that the main role in the development of proteinuria is played by damage to the podocytes and their slit diaphragm. The podocyte damage mechanism in preeclampsia is connected to free VEGF and nitric oxide (NO) deficiency, and an increased concentration of endothelin-1 and oxidative stress. From national cohort studies, we know that women who had preeclampsia in at least one pregnancy carried five times the risk of developing end-stage renal disease (ESRD) when compared to women with physiological pregnancies. The focal segmental glomerulosclerosis (FSGS) is the dominant histopathological lesion in women with a history of PE. The kidney’s podocytes are not subject to replacement or proliferation. Podocyte depletion exceeding 20% resulted in FSGS, which is a reason for the later development of ESRD. In this review, we present the mechanism of kidney (especially podocytes) injury in preeclampsia. We try to explain how this damage affects further changes in the morphology and function of the kidneys after pregnancy.
Sperm Motility of Mice under Simulated Microgravity and Hypergravity
Authors: Ogneva Irina V. , Usik Maria A. , Biryukov Nikolay S. , Zhdankina Yuliya S.
Published: July 2020

For deep space exploration, reproductive health must be maintained to preserve the species. However, the mechanisms underlying the effect of changes in gravity on male germ cells remain poorly understood. The aim of this study was to determine the effect of simulated micro- and hypergravity on mouse sperm motility and the mechanisms of this change. For 1, 3 and 6 h, mouse sperm samples isolated from the caudal epididymis were subjected to simulated microgravity using a random position machine and 2g hypergravity using a centrifuge. The experimental samples were compared with static and dynamic controls. The sperm motility and the percentage of motile sperm were determined using microscopy and video analysis, cell respiration was determined by polarography, the protein content was assessed by Western blotting and the mRNA levels were determined using qRT-PCR. The results indicated that hypergravity conditions led to more significant changes than simulated microgravity conditions: after 1 h, the speed of sperm movement decreased, and after 3 h, the number of motile cells began to decrease. Under the microgravity model, the speed of movement did not change, but the motile spermatozoa decreased after 6 h of exposure. These changes are likely associated with a change in the structure of the microtubule cytoskeleton, and changes in the energy supply are an adaptive reaction to changes in sperm motility.
Bio-inspired Surface Texture Modification as a Viable Feature of Future Aquatic Antifouling Strategies: A Review
Authors: Richards Chloe , Slaimi Asma , O’Connor Noel E. , Barrett Alan , Kwiatkowska Sandra , Regan Fiona
Published: July 2020

The imitation of natural systems to produce effective antifouling materials is often referred to as “biomimetics”. The world of biomimetics is a multidisciplinary one, needing careful understanding of “biological structures”, processes and principles of various organisms found in nature and based on this, designing nanodevices and nanomaterials that are of commercial interest to industry. Looking to the marine environment for bioinspired surfaces offers researchers a wealth of topographies to explore. Particular attention has been given to the evaluation of textures based on marine organisms tested in either the laboratory or the field. The findings of the review relate to the numbers of studies on textured surfaces demonstrating antifouling potential which are significant. However, many of these are only tested in the laboratory, where it is acknowledged a very different response to fouling is observed.
Targeting Cannabinoid Receptors: Current Status and Prospects of Natural Products
Authors: An Dongchen , Peigneur Steve , Hendrickx Louise Antonia , Tytgat Jan
Published: July 2020

Cannabinoid receptors (CB1 and CB2), as part of the endocannabinoid system, play a critical role in numerous human physiological and pathological conditions. Thus, considerable efforts have been made to develop ligands for CB1 and CB2, resulting in hundreds of phyto- and synthetic cannabinoids which have shown varying affinities relevant for the treatment of various diseases. However, only a few of these ligands are clinically used. Recently, more detailed structural information for cannabinoid receptors was revealed thanks to the powerfulness of cryo-electron microscopy, which now can accelerate structure-based drug discovery. At the same time, novel peptide-type cannabinoids from animal sources have arrived at the scene, with their potential in vivo therapeutic effects in relation to cannabinoid receptors. From a natural products perspective, it is expected that more novel cannabinoids will be discovered and forecasted as promising drug leads from diverse natural sources and species, such as animal venoms which constitute a true pharmacopeia of toxins modulating diverse targets, including voltage- and ligand-gated ion channels, G protein-coupled receptors such as CB1 and CB2, with astonishing affinity and selectivity. Therefore, it is believed that discovering novel cannabinoids starting from studying the biodiversity of the species living on planet earth is an uncharted territory.
Signaling of Tumor-Derived sEV Impacts Melanoma Progression
Authors: Zebrowska Aneta , Widlak Piotr , Whiteside Theresa , Pietrowska Monika
Published: July 2020

Small extracellular vesicles (sEV or exosomes) are nanovesicles (30–150 nm) released both in vivo and in vitro by most cell types. Tumor cells produce sEV called TEX and disperse them throughout all body fluids. TEX contain a cargo of proteins, lipids, and RNA that is similar but not identical to that of the “parent” producer cell (i.e., the cargo of exosomes released by melanoma cells is similar but not identical to exosomes released by melanocytes), possibly due to selective endosomal packaging. TEX and their role in cancer biology have been intensively investigated largely due to the possibility that TEX might serve as key component of a “liquid tumor biopsy.” TEX are also involved in the crosstalk between cancer and immune cells and play a key role in the suppression of anti-tumor immune responses, thus contributing to the tumor progression. Most of the available information about the TEX molecular composition and functions has been gained using sEV isolated from supernatants of cancer cell lines. However, newer data linking plasma levels of TEX with cancer progression have focused attention on TEX in the patients’ peripheral circulation as potential biomarkers of cancer diagnosis, development, activity, and response to therapy. Here, we consider the molecular cargo and functions of TEX as potential biomarkers of one of the most fatal malignancies—melanoma. Studies of TEX in plasma of patients with melanoma offer the possibility of an in-depth understanding of the melanoma biology and response to immune therapies. This review features melanoma cell-derived exosomes (MTEX) with special emphasis on exosome-mediated signaling between melanoma cells and the host immune system.
Circulating Ouabain Modulates Expression of Claudins in Rat Intestine and Cerebral Blood Vessels
Authors: Markov Alexander G. , Fedorova Arina A. , Kravtsova Violetta V. , Bikmurzina Anastasia E. , Okorokova Larisa S. , Matchkov Vladimir V. , Cornelius Valeria , Amasheh Salah , Krivoi Igor I.
Published: July 2020

The ability of exogenous low ouabain concentrations to affect claudin expression and therefore epithelial barrier properties was demonstrated previously in cultured cell studies. We hypothesized that chronic elevation of circulating ouabain in vivo can affect the expression of claudins and tight junction permeability in different tissues. We tested this hypothesis in rats intraperitoneally injected with ouabain (1 μg/kg) for 4 days. Rat jejunum, colon and brain frontal lobes, which are variable in the expressed claudins and tight junction permeability, were examined. Moreover, the porcine jejunum cell line IPEC-J2 was studied. In IPEC-J2-cells, ouabain (10 nM, 19 days of incubation) stimulated epithelial barrier formation, increased transepithelial resistance and the level of cSrc-kinase activation by phosphorylation, accompanied with an increased expression of claudin-1, -5 and down-regulation of claudin-12; the expression of claudin-3, -4, -8 and tricellulin was not changed. In the jejunum, chronic ouabain increased the expression of claudin-1, -3 and -5 without an effect on claudin-2 and -4 expression. In the colon, only down-regulation of claudin-3 was observed. Chronic ouabain protected the intestine transepithelial resistance against functional injury induced by lipopolysaccharide treatment or by modeled acute microgravity; this regulation was most pronounced in the jejunum. Claudin-1 was also up-regulated in cerebral blood vessels. This was associated with reduction of claudin-3 expression while the expression of claudin-5 and occludin was not affected. Altogether, our results confirm that circulating ouabain can functionally and tissue-specifically affect barrier properties of epithelial and endothelial tissues via Na,K-ATPase-mediated modulation of claudins expression.
Adenosine A2A and A3 Receptors Are Able to Interact with Each Other. A Further Piece in the Puzzle of Adenosine Receptor-Mediated Signaling
Authors: Lillo Alejandro , Martínez-Pinilla Eva , Reyes-Resina Irene , Navarro Gemma , Franco Rafael
Published: July 2020

The aim of this paper was to check the possible interaction of two of the four purinergic P1 receptors, the A2A and the A3. Discovery of the A2A–A3 receptor complex was achieved by means of immunocytochemistry and of bioluminescence resonance energy transfer. The functional properties and heteromer print identification were addressed by combining binding and signaling assays. The physiological role of the novel heteromer is to provide a differential signaling depending on the pre-coupling to signal transduction components and/or on the concentration of the endogenous agonist. The main feature was that the heteromeric context led to a marked decrease of the signaling originating at A3 receptors. Interestingly from a therapeutic point of view, A2A receptor antagonists overrode the blockade, thus allowing A3 receptor-mediated signaling. The A2A–A3 receptor heteromer print was detected in primary cortical neurons. These and previous results suggest that all four adenosine receptors may interact with each other. Therefore, each adenosine receptor could form heteromers with distinct properties, expanding the signaling outputs derived from the binding of adenosine to its cognate receptors.
Attributes of Yellow Traps Affecting Attraction of Diaphorina citri (Hemiptera: Liviidae)
Authors: Allan Sandra A. , George Justin , Stelinski Lukasz L. , Lapointe Stephen L.
Published: July 2020

Laboratory assays were conducted to evaluate responses of Diaphorina citri to various aspects of visual cues associated with traps in an effort to improve trap effectiveness. Addition of white or UV violet but not yellow light-emitting diodes (LEDs) increased attraction to standard yellow adhesive traps moderately (11–17%), with no difference in attraction between white or UV violet LEDs. Addition of a black border on yellow traps enhanced collections. However, there were no differences between attraction to black patterns on traps. Comparisons were made between different commercial paints, some with UV-reflecting properties or fluorescence. A yellow paint with UV reflectance, used for painting bird decoys (decoy yellow), was more attractive than the standard yellow Olson sticky trap. Addition of white or green pigment to increase intensity or enhance green reflectance, respectively, did not increase attraction. Alteration of reflectance of Olson traps with addition of UV-reflecting or fluorescent pigments did not enhance attraction of D. citri. In field comparisons, decoy yellow and fluorescent yellow sticky traps were more attractive to D. citri than Olson yellow.
Bragg Reflections of Oblique Water Waves by Periodic Surface-Piercing and Submerged Breakwaters
Authors: Tseng I-Fan , You Chi-Shian , Tsai Chia-Cheng
Published: July 2020

The Bragg reflections of oblique water waves by periodic surface-piercing structures over periodic bottoms are investigated using the eigenfunction matching method (EMM). Based on the assumption of small wave amplitude, the linear wave theory is employed in the solution procedure. In the step approximation, the surface-piercing structures and the bottom profiles are sliced into shelves separated by abrupt steps. For each shelf, the solution is composed of eigenfunctions with unknown coefficients representing the wave amplitudes. Upon applying the conservations of mass and momentum, a system of linear equations is obtained and is then solved by a sparse-matrix solver. The proposed EMM is validated by several examples in the literature. Then, the method is applied to solve Bragg reflections of oblique water waves by various surface-piercing structures over periodic bottoms. From the numerical experiments, Bragg’s law of oblique waves was used to predict the occurrences of Bragg resonance.
Fine Dust and Sustainable Supply Chain Management in Port Operations: Focus on the Major Cargo Handled at the Dry Bulk Port
Authors: Hwang Jeho , Kim Sihyun
Published: July 2020

Local residents living adjacent to ports are directly affected by the fine dust generated from the port operations. There is a need to prepare detailed measures according to cargo type given the high correlation between the types of dust-producing cargo primarily managed at ports and local industries. This study attempts to establish the attributes of the cargo handled at ports and the relationship between supply chains built for local key industries and the air quality of the local community. It aims to ascertain which cargo needs managing preemptively at the local level, based on the major cargo types handled in a port. A correlation analysis and Granger causality test were performed to investigate the causality between the factor of cargo and fine dust concentrations. The results in this study indicate the necessity for intensive management of scrap metal cargo among the major cargo handled at the target port, which confirms the large effect of management on fine dust reduction, as well as on reduction efficiency. The results suggest requirements to expand the regulations on the emissions of supply chains by cargo type, not by industry type. Additionally, it is required to minimize the blind spots of management and form an eco-friendly supply chain by introducing green technology. The preparation of emission control measures is also necessary. The findings provide useful insights for the sustainable operations of the local supply chain around the target port and will help the strategic agenda for future improvement.
Time-Frequency Based Dynamics of Decoupling or Integration between Islamic and Conventional Equity Markets
Authors: Anas Muhammad , Mujtaba Ghulam , Nayyar Sadaf , Ashfaq Saira
Published: July 2020

This paper investigates the decoupling and integration between the region-wise (Asia, Europe, Africa and the Americas) developed and emerging market’s equity pairs of Islamic and conventional stock returns with the focus on multi-horizons. In doing so, daily wavelet and ADCC-based stock returns correlations are estimated to capture the dynamics of time-frequency and the time-domain based correlations, respectively. The findings of this study indicate that at the short-term horizon, the all selected emerging and developed Islamic and conventional equity markets across all regions depict a high positive correlation, suggesting a rejection of the decoupling hypothesis. However, it is accepted for some of the developed markets of the Pacific region (Hong Kong and New Zealand), Europe (Ireland, Denmark and Spain) and emerging markets of Asia (China), Europe (Czech Republic) and Americas (Argentina and Peru) at a medium-term horizon. Moreover, in an examination of the comparative behaviors of the wavelet and ADCC-based Islamic-conventional correlations, the observed transitional behavior has been exemplified as the difference between the time-frequency and time-domain analysis. This study provides fruitful insights for investors who opt for cross-asset allocation and seek maximum portfolio diversification benefits.
Challenges of Food Waste Governance: An Assessment of European Legislation on Food Waste and Recommendations for Improvement by Economic Instruments
Authors: Garske Beatrice , Heyl Katharine , Ekardt Felix , Weber Lea , Gradzka Wiktoria
Published: July 2020

Food is wasted throughout the entire food supply chain—from agricultural production to the household level. This has negative impacts on natural resources and the environment. At the same time, food waste is undermining the global target of food security. In turn, reducing food waste can minimise the environmental effects of agriculture on climate, biodiversity, soils, water bodies and the atmosphere. All of this is reflected in the fact that food waste is subject to various legal acts of the European Union and that it is also a major subject in the new EU Farm to Fork Strategy from May 2020. Supported by an analysis of the diffuse empirical data on food waste, the purpose of this article is to analyse the current EU legislation on food waste and its reduction to answer the following research questions: How is food waste integrated into European policies? What is the impact of European legislation on food waste? Is European legislation sufficient to trigger not only food waste reduction but also comprehensive changes in the agricultural and food sector to support global climate and environmental targets as set in the Paris Agreement and the Convention on Biological Diversity? Which instruments are the most suitable to do so? Methodologically, a qualitative governance analysis is applied. It is found that relevant legal acts for governing food waste include circular economy and waste law, the Common Agricultural Policy and the Common Fisheries Policy as well as food law, while international environmental targets serve as an overarching measure for governance analysis. The legal analysis shows that existing legislation lacks steering effect to significantly reduce food waste. To overcome current governance problems, the article introduces economic policy instruments. It is concluded that quantity control focusing on overarching parameters such as fossil fuels or animal-derived products has not only the potential to reduce food waste by increasing food prices but can also address the multiple interlinked environmental challenges of the agricultural and food sector.
Land-Use Changes in the Canary Archipelago Using the CORINE Data: A Retrospective Analysis
Authors: Naranjo Gómez José Manuel , Lousada Sérgio , Garrido Velarde Jacinto Garrido , Castanho Rui Alexandre , Loures Luís
Published: July 2020

The relationships between territorial governance and the pursuit of sustainable development are evidenced to be critical. Exploratory tools, like Geographic Information Systems (GIS), enable us to comprehend the patterns, dynamics, and parameters of land-use changes over the years. The results from such studies could be used in the design of a sustainable territorial governance strategy. Contextually, a study has been conducted based on the changes that occurred in land uses in the Canary Archipelago in the years 1990, 2000, 2012, and 2018 using CORINE (Coordination of Information on the Environment) data. Even if most of the land uses have been stable over the analyzed period, the investigation shows a decrease in agricultural areas. By contrast, it is possible to verify an increase in semi-natural areas and urban agglomerations. Moreover, the authors believe that an assessment of the land-use changes on these ultra-peripheral areas will also enable us to disclose some obstacles and opportunities for sustained development.
Plasma Jets Fabricated in Low-Temperature Cofired Ceramics for Gold Nanoparticles Synthesis
Authors: Rac-Rumijowska Olga , Macioszczyk Jan , Matusiak Tomasz , Golonka Leszek , Teterycz Helena
Published: July 2020

In this article, we present a development of atmospheric pressure plasma jets (APPJs) for modification of liquid solutions. APPJs were fabricated in low temperature cofired ceramics (LTCC) technology. During the measurements, plasma jets worked under various flowing gases, which can be used to produce plasma activated water. In addition, owing to the plasma treatment, it was possible to decrease the time of a synthesis of gold nanoparticles (AuNPs) without the use of additional hazardous reagents. The mechanism of gold nanoparticles formation in cold nitrogen plasma is also presented.