bluuebunny/crossref_metadata_2025 huggingface dataset:
Open Dialogue: A case study on the influence of sharing or withholding reflections during a network meeting
Authors: van Dieren Albert , Clavero Corine
Published: December 2022

In Open Dialogue, sharing of reflections by professionals constitutes an important contribution to promoting a polyphonic dialogue between participants. In the inner dialogue, past and future influence the present moment. In this study, we explore the influence of sharing or withholding reflections by professionals on the interplay between inner and outer dialogue. A case study was used with a multi-perspective methodology, which combined video recordings of a network meeting and interviews by using video-stimulated recall with the clients separately, and social workers together afterward. We found that the sharing of reflections by professionals stimulates the inner dialogue and creates an opening for sharing these in the outer dialogue. In addition, we observed that when reflections are withheld, the client's inner dialogue still continues, but their inner dialogue was not shared in the outer dialogue.
Validity and reliability of the Chinese version of the Young Positive Schema Questionnaire
Authors: Chi Danni , Du Xiangju , Ma Hong , Wang Yubo , Zhang Yuanyuan , Zhong Haiyun
Published: December 2022

Introduction

The Young Positive Schema Questionnaire (YPSQ) examines early adaptive or positive schemas as a counterpart to early maladaptive ones. This study investigated the validity and reliability of the Chinese version of the YPSQ (CYPSQ).

Methods

A convenient community sample of 634 individuals, most of whom were college students, were recruited through a mobile survey. R Careless was used to screen for careless responses. A final sample of 336 was obtained. Confirmatory factor analyses (CFA) of the CYPSQ were conducted using SPSS AMOS 25. Other statistical analyses were conducted using SPSS 25.

Results

An 11-factor CYPSQ was identified with an acceptable factor structure (χ2/df = 2.13, SRMR = 0.04, RMSEA = 0.06, GFI = 0.80, TLI = 0.90, and CFI = 0.91). Convergent and discriminant validity were confirmed in most aspects. Concurrent validity was evident with resilience, life satisfaction, depression, and anxiety. Internal reliability was satisfied as the Cronbach’s alphas of the 11 factors of the CYPSQ ranged between 0.70 and 0.88.

Conclusion

The findings supported the reliability and validity of the CYPSQ in Mainland China.
Development of a multi-component tobacco cessation training package utilizing multiple approaches of intervention development for health care providers and patients attending non-communicable disease clinics of Punjab, India
Authors: Bhatt Garima , Goel Sonu , Grover Sandeep , Medhi Bikash , Singh Gurmandeep , Gill Sandeep Singh , Swasticharan Leimapokpam , Singh Rana J.
Published: December 2022

Background

Providing patients with personalized tobacco cessation counseling that is culturally sensitive, and disease-specific from healthcare providers (HCPs) as part of their routine consultations is an approach that could be incorporated, using existing healthcare systems such as the Non-Communicable Disease (NCD) clinics. This paper describes the development of a multi-component culturally tailored, patient-centric, disease-specific tobacco cessation package utilizing multiple approaches of intervention development for healthcare providers and patients attending these clinics in Punjab, India, along with a proposed framework for implementation.

Methods

The proposed intervention package was developed in 6 stages. These included a review of literature for identifying successful cessation interventions for ethnic minority groups, co-production of the package with all stakeholders involved via a series of consultative meetings and workshops, understanding contextual factors of the state and ‘factor-in’ these in the package, pre-test of the package among HCPs and tobacco users using in-depth interviews, micro detailing and expansion of the package by drawing on existing theories of the Cascade Model and Trans-Theoretical Model and developing an evolving analysis plan through real-world implementation at two pilot districts by undertaking a randomized controlled trial, assessing implementer's experiences using a mixed-method with a primary focus on qualitative and economic evaluation of intervention package.

Results

A multi-component package consisting of a booklet (for HCPs), disease-specific pamphlets and short text messages (for patients; bilingual), and an implementation framework was developed using the 6-step process. A major finding from the in-depth interviews was the need for a specific capacity-building training program on tobacco cessation. Therefore, using this as an opportunity, we trained the in-service human resource and associated program managers at the state and district-level training workshops. Based on the feedback, training objectives were set and supported with copies of intervention package components. In addition, the role and function of each stakeholder were defined in the proposed framework.

Conclusion

Consideration of tobacco users' socio-cultural and patient-centric approach makes a robust strategy while developing and implementing an intervention providing an enlarged scope to improve care services for diversified socio-cultural communities.
An NLP tool for data extraction from electronic health records: COVID-19 mortalities and comorbidities
Authors: BuHamra Sana S. , Almutairi Abdullah N. , Buhamrah Abdullah K. , Almadani Sabah H. , Alibrahim Yusuf A.
Published: December 2022

Background

The high infection rate, severe symptoms, and evolving aspects of the COVID-19 pandemic provide challenges for a variety of medical systems around the world. Automatic information retrieval from unstructured text is greatly aided by Natural Language Processing (NLP), the primary approach taken in this field. This study addresses COVID-19 mortality data from the intensive care unit (ICU) in Kuwait during the first 18 months of the pandemic. A key goal is to extract and classify the primary and intermediate causes of death from electronic health records (EHRs) in a timely way. In addition, comorbid conditions or concurrent diseases were retrieved and analyzed in relation to a variety of causes of mortality.

Method

An NLP system using the Python programming language is constructed to automate the process of extracting primary and secondary causes of death, as well as comorbidities. The system is capable of handling inaccurate and messy data, this includes inadequate formats, spelling mistakes and mispositioned information. A machine learning decision trees method is used to classify the causes of death.

Results

For 54.8% of the 1691 ICU patients we studied, septic shock or sepsis-related multiorgan failure was the leading cause of mortality. About three-quarters of patients die from acute respiratory distress syndrome (ARDS), a common intermediate cause of death. An arrhythmia (AF) disorder was determined to be the strongest predictor of intermediate cause of death, whether caused by ARDS or other causes.

Conclusion

We created an NLP system to automate the extraction of causes of death and comorbidities from EHRs. Our method processes messy and erroneous data and classifies the primary and intermediate causes of death of COVID-19 patients. We advocate arranging the EHR with well-defined sections and menu-driven options to reduce incorrect forms.
Influence of COVID-19 for delaying the diagnosis and treatment of pulmonary tuberculosis–Tianjin, China
Authors: Zhang Guoqin , Yu Yanming , Zhang Wenqian , Shang Jian , Chen Shengyu , Pang Xuewen , Oeltmann John E. , Moonan Patrick K. , Chen Mingting , Zhang Fan
Published: December 2022

Background

The COVID-19 pandemic has disrupted the diagnosis, treatment, and care for tuberculosis (TB). Delays in seeking TB care may result in increased community transmission and unfavorable treatment outcomes. We sought to understand the influence of the COVID-19 pandemic on the proportion of patients with TB who delayed seeking the diagnosis and care for TB and explore the reasons for their postponement.

Methods

We surveyed a representative sample of outpatients treated for pulmonary TB from June to November 2020 using an anonymous standardized questionnaire. Multivariable logistic regression was used to calculate adjusted odds ratios (aOR) and 95% confidence intervals (CIs) of factors associated with the postponement of TB care. We used routinely collected surveillance data to assess trends of TB reports before and after the emergence of COVID-19 (2017–2019 vs. 2020–2022) in Tianjin, China.

Results

Among 358 participants who were diagnosed with pulmonary TB during the COVID-19 response, 61 (17%) postponed seeking TB diagnosis due to COVID-19, with 39 (64%) citing fear as the primary reason. Female sex (aOR:2.0; 95% CI: 1.1–3.7), previous antituberculosis treatment (aOR:3.2; 95%CI: 1.4–7.6), and TB diagnosis during the first-level response (aOR = 3.2, 1.7–6.2) were associated with the postponement. Among all 518 participants receiving antituberculosis treatment, 57 (11%) had postponed their regular healthcare visits due to COVID-19, 175 (34%) received no treatment supervision, and 32 (6%) experienced treatment interruption. Compared to 2017–2019, reported pulmonary TB declined by 36.8% during the first-level response to COVID-19, 23.5% during the second-level response, 14% during the third-level response in 2020, and 4.3% in 2021.

Conclusion

The COVID-19 response reduced the number of people who sought and received diagnosis, treatment, and care for TB in Tianjin, China. Integrative programs to ensure access and continuity of TB services should be considered and dual testing for SARS-CoV-2 and M. tuberculosis may facilitate finding cases.
The Lesion Method: What Individual Patients Can Teach Us About the Brain
Authors: McCurdy Ryan , Clough Sharice , Edwards Malcolm , Duff Melissa
Published: December 2022

Scientists who study the brain try to understand how it performs everyday behaviors like language, memory, and emotion. Scientists learn a lot by studying how these behaviors change when the brain is damaged. Over the past 200 years, they have made many discoveries by studying individuals with brain damage. For example, one patient could not form sentences after damaging a specific area of his brain. The scientist who studied him concluded that the damaged brain area was important for producing speech. This approach is called the lesion method, and it has taught us a lot about the brain. In this article, we introduce five patients throughout history who forever changed our understanding of the brain. We describe how researchers use these early discoveries to ask new questions about the brain, and we conclude by discussing how the lesion method is used today.
Understanding system barriers and facilitators in transnational clinical cancer research: The value of rapid and multimodal ethnographic inquiry
Authors: Bright Kristin
Published: December 2022

Introduction

In middle and low resource countries worldwide, up to 70% of breast cancer cases are diagnosed as locally advanced (stages IIB-IIIC). Delays in referral from primary to specialty care have been shown to prolong routes to diagnosis and may be associated with higher burdens of advanced disease, but specific clinical and organizational barriers are not well understood.

Methods

This article reports on the use of rapid ethnographic research (RER) within a largescale clinical trial for locally advanced breast cancer (LABC) in India, Mexico, South Africa, and the US. Our purpose is twofold. First, we demonstrate the value of ethnography as a mode of evaluative listening: appraising the perspectives of diverse patients and clinicians regarding prolonged routes to LABC diagnosis and treatment. Second, we show the value of ethnography as a compass for navigating among discrepant clinical research styles, IRB protocols, and institutional norms and practices. We discuss advantages and limits involved in each use of RER.

Results

On the one hand, ethnographic interviews carried out before and during the clinical trial enabled more regular communication among investigators and research sites. On the other hand, the logistics of doing the trial placed limits on the extent and duration of inductive, immersive inquiry characteristic of traditional fieldwork. As a partial solution to this problem, we developed a multimodal ethnographic research (MER) approach, an augmentation of video-chat, phone, text, and email carried out with, and built upon the initial connections established in, the in-person fieldwork. This style has its limits; but it did allow us to materially improve the ways in which the medical research proceeded.

Discussion

In conclusion, we highlight the value of not deferring to a presumed incommensurability of ethnographic fieldwork and clinical trialwork while still being appropriately responsive to moments when the two approaches should be kept apart.
Mesh Adaptation for Simulating Lateral Jet Interaction Flow
Authors: Tian Shuling , Peng Zongzi
Published: December 2022

Under the condition of supersonic incoming flow, a missile lateral jet flow field has complex flow structures, such as a strong shock wave, an unsteady vortex and flow separation. In order to improve ability to capture complex flow structures in numerical simulation of lateral jets, this paper proposes a combined-grid adaptive method. When combined with finite volume approximation of second-order and h-type adaptive technology, our method was verified by numerical experiments, which shows that wave structure and vortex structure in the jet flow field can be effectively captured at the same time. In comparison of uniformly refined mesh results, it was found that accuracy of computed results and resolution of characteristic flow structures were significantly improved after mesh adaptation. In comparison of the pressure coefficient, it was found that the error between the adaptive mesh and the uniformly refined mesh was smaller, and the maximum errors of the base grid, adaptive grid and uniformly refined grid were 92.1% and 12.3%.
Size-Controllable Prussian Blue Nanoparticles Using Pluronic Series for Improved Antioxidant Activity and Anti-Inflammatory Efficacy
Authors: Oh Hyeryeon , Lee Jin Sil , Sung Daekyung , Yang Siyoung , Choi Won Il
Published: December 2022

Prussian blue (PB) is a metal cluster nanoparticle (NP) of cyanide-bridged iron(II)–iron(III) and exhibits a characteristic blue color. Its peroxidase-, catalase-, and superoxide-dismutase-like activities effectively remove excess reactive oxygen species that induce inflammation and tumorigenesis. However, the dispersion of PB NPs is not sufficiently stable for their application in the biomedical field. In this study, we developed Pluronic-stabilized Prussian blue nanoparticles (PB/Plu NPs) using a series of Pluronic triblock copolymers as a template material for PB NPs. Considering the hydrophilic–lipophilic balance (HLB) values of the Pluronic series, including F68, F127, L35, P123, and L81, the diameters of the PB/Plu NPs decreased from 294 to 112 nm with decreasing HLB values. The smallest PB NP stabilized with Pluronic P123 (PB/PP123 NP) showed the strongest antioxidant and anti-inflammatory activities and wound-healing efficacy because of its large surface area. These results indicated that the spatial distribution of PB NPs in the micelles of Pluronic greatly improved the stability and reactive oxygen species scavenging activity of these NPs. Therefore, PB/Plu NPs using U.S.-FDA-approved Pluronic polymers show potential as biocompatible materials for various biomedical applications, including the treatment of inflammatory diseases in the clinic.
Optimization of a Microwave Assisted Extraction Method for Maximum Flavonols and Antioxidant Activity of Onion Extracts
Authors: V. González-de-Peredo Ana , Vázquez-Espinosa Mercedes , Espada-Bellido Estrella , Ferreiro-González Marta , F. Barbero Gerardo , Palma Miguel , Carrera Ceferino
Published: December 2022

Nowadays, consumers demand bioactive foods that have the potential to limit the risk of suffering from several medical conditions. Onions present these desirable capabilities owing to its high content in antioxidant bioactive compounds. This work has used a Box–Behnken design with a response surface methodology to determine the best conditions in which to extract the polyphenols that are found in onions. Two extraction methods—one for the extraction of total flavonols and another one intended to obtain extracts with the highest possible antioxidant activity—have been developed and optimized. The following factors have been studied: temperature, %methanol in water, solvent pH, and sample–solvent volumetric ratio. The optimal conditions for the extraction of flavonols were 93.8% methanol in water, pH 2, 50 °C extraction temperature and 0.2:17.9 g:mL sample–solvent ratio. The best antioxidant activity levels were registered when using 74.2% methanol in water, pH 2, 99.9 °C extraction temperature and 0.2:18.2 g:mL sample–solvent ratio. Both optimized methods used short extraction times, and presented good precision levels and successful results when used with an assortment of onion varieties. According to total flavonols and antioxidant activity data, with 7.557 ± 0.3261 and 12.08 ± 0.0379 mg g−1, respectively, the developed methods achieved comparable or even superior results to those obtained by other authors.
The Safety and Effectiveness of Early, Progressive Weight Bearing and Implant Choice after Traumatic Lower Extremity Fracture: A Systematic Review
Authors: Flowers Daniel W. , McCallister Erin , Christopherson Ricki , Ware Erin
Published: December 2022

The goal of this systematic review was to examine existing evidence on the effectiveness of early, progressive weight bearing on patients after traumatic lower extremity fractures and relate these findings to device/implant choice. A search of the literature in PubMed/Medline, Embase, Web of Science, and the Cochrane Library was performed through January 2022. Randomized controlled trials and non-randomized, prospective longitudinal investigations of early, progressive weight bearing in skeletally mature adults after traumatic lower extremity fracture were included in the search, with 21 publications included in the final analysis. A summary of the loading progressions used in each study, along with the primary and additional outcomes, is provided. The progression of weight bearing was variable, dependent on fracture location and hardware fixation; however, overall outcomes were good with few complications. Most studies scored “high” on the bias tools and were predominately performed without physical therapist investigators. Few studies have investigated early, progressive weight bearing in patients after traumatic lower extremity fractures. The available clinical evidence provides variable progression guidelines. Relatively few complications and improved patient function were observed in this review. More research is needed from a rehabilitation perspective to obtain graded progression recommendations, informed by basic science concepts and tissue loading principles.
Simulation-Based Designing of Suitable Stimulation Factors for Presenting Two Phosphenes Simultaneously to Lower Side of Field of View
Authors: Kanamaru Manami , Tan Phan Xuan , Kamioka Eiji
Published: December 2022

Using a phosphene has been discussed as a means of informing the visually impaired of the position of an obstacle. Obstacles underfoot have a risk, so it is necessary to inform the visually impaired. A previous study clarified a method of presenting phosphene in three directions in the lower vision; however, the simultaneous presentation of these phosphenes has not been discussed. Another study discussing the effect of electrical interference when stimulating the eyeball with multiple electrodes indicated that it is important to select appropriate stimulation factors to avoid this effect. However, when the stimulation electrodes are arranged remarkably close, there is a high possibility that the stimulus factor presented in the previous study will not apply. In this study, a method for simultaneously presenting phosphenes in the lower vision is presented. The electrode arrangements reported in the previous study to present phosphene in the lower field of vision are used, and the difficulty in the simultaneous presentation of multiple phosphenes in the lower vision is the focus. In this paper, the method of designing the stimulation factors is discussed numerically when the electrodes are arranged remarkably close. As a result, it is shown that stimulation factors different from the previous research were appropriate depending on the distance between the electrodes.
Exosomes from the Uterine Cavity Mediate Immune Dysregulation via Inhibiting the JNK Signal Pathway in Endometriosis
Authors: Jiang Ying , Chai Xiaoshan , Chen Shengnan , Chen Zhaoying , Tian Hao , Liu Min , Wu Xianqing
Published: December 2022

Endometriosis is a chronic inflammatory disease with an uncertain pathogenesis. Peritoneal immune dysregulation plays an important role in the pathogenesis of endometriosis. Exosomes are messengers of intercellular communication. This study mainly investigated the role of exosomes from the uterine cavity in endometriosis. Exosomes of the uterine aspirate fluid were isolated and cocultured with macrophages for 48 h. Flow cytometry was used to detect macrophage polarization. A Human MAPK Phosphorylation Antibody Array and Western blot were used to detect the phosphorylation of the MAPK pathway. A microRNA sequencing analysis was used to detect differentially expressed miRNAs. Our research found that exosomes of the uterine aspirate fluid from endometriosis could reduce the proportion of CD80+ macrophages. Additionally, it could inhibit the expression of P-JNK in macrophages. However, the JNK activator anisomycin could increase the proportion of CD80+ macrophages. In addition, exosomes of the uterine aspirate fluid from endometriosis could promote the migration and invasion of endometrial stromal cells by acting on macrophages. The expression of miR-210-3p was increased in both exosomes and the eutopic endometrium in patients with endometriosis through miRNA sequencing, which could also reduce the proportion of CD80+ macrophages. In summary, we propose that exosomes from the uterine cavity in patients with endometriosis may affect the phenotype of macrophages by inhibiting the JNK signaling pathway, thus mediating the formation of an immunological microenvironment conducive to the development of endometriosis.
The Attitude of the General Dentist in the Republic of Croatia toward Treating Children
Authors: Gavić Lidia , Nikolić Ivana , Sidhu Sharanbir K. , Jerković Daniel , Tadin Antonija
Published: December 2022

The aim of this study was to determine the attitude of general dentists in the Republic of Croatia toward working with children. The questionnaire survey involved 361 general dentists. The questionnaire was divided into three parts and contained 34 questions. The first part of the questionnaire survey contained demographic data questions. In the second part, dentists answered questions about the characteristics of the dental offices where they work, the materials that they mostly use, and how they work with children. The third part of the questionnaire referred to attitudes about working and treating young children and consisted of 12 statements, to which the answers were offered on a Likert scale from one to five. This study showed that only 12.46% of dentists have a positive attitude toward treating children and 30.19% of dentists have a negative attitude toward treating children. In addition, the attitude toward working with children correlates with both the knowledge they acquired during dental studies (R = 0.355; p ≤ 0.001) and gender (R = −0.103; p = 0.035). This study confirmed that women have a more often positive attitude toward treating young children. There was a major correlation between the level of education and positive attitudes toward treating children. The child’s non-cooperative behavior was the main reason why general dentists refuse to work with children.
The Association of Junk Food Consumption with Preadolescents’ Environmental Influences: A School-Based Epidemiological Study in Greece
Authors: Gketsios Ioannis , Tsiampalis Thomas , Foscolou Alexandra , Vassilakou Tonia , Kanellopoulou Aikaterini , Notara Venetia , Antonogeorgos George , Rojas-Gil Andrea , Kornilaki Ekaterina , Lagiou Areti , Panagiotakos Demosthenes , Kosti Rena
Published: December 2022

The aim of the present study was to evaluate the impact of environmental influences on Greek preadolescents’ junk food consumption. A cross-sectional study, was conducted among 1718 preadolescents (mean (standard deviation(SD)) age: 11.2(0.8) years old; 54% girls) and their parents, during the school years 2014–2016. Parental and child characteristics were collected anonymously, through self-administered and validated questionnaires. Among others, junk food consumption was recorded, classifying children as low, moderate, and high consumers. The majority of the preadolescents were classified as at least moderate junk food consumers, while almost 3/10 children were classified as high junk food consumers. A significantly lower junk food consumption was observed among preadolescents with a healthier family environment, consisting of normal-weight parents who consume junk foods less frequently, prefer home-cooked meals and adhere more to the Mediterranean diet, while more frequent family meals were also associated with lower junk food consumption. In addition, influence from teachers and participation in extracurricular sports activities were significantly associated with lower junk food consumption, while advertisements were found to have a significant negative impact on preadolescents’ eating habits. Notwithstanding, peers were not found to influence their dietary choices in terms of junk food consumption. Both parents and teachers seem to be positive influencers on preadolescents’ low junk food consumption. The detrimental role of advertisements on junk food consumption is reconfirmed, while peers’ influence is not significant on junk food consumption. The need for urgent public health initiatives for the promotion of healthy dietary habits among preadolescents is warranted.
Outcomes, Return to Sport, and Failures of MPFL Reconstruction Using Autografts in Children and Adolescents with Recurrent Patellofemoral Instability: A Systematic Review
Authors: Migliorini Filippo , Maffulli Nicola , Bell Andreas , Betsch Marcel
Published: December 2022

Introduction: This study systematically reviews and updates the current evidence on the outcomes of medial patellofemoral ligament (MPFL) reconstruction using autografts in children and adolescents with recurrent patellofemoral instability. The outcomes of interest were improvements in patient reported outcomes measures (PROMs), return to sport rates, and the rates of surgical failure. Methods: This systematic review was performed according to the 2020 PRISMA guidelines. The following electronic databases were accessed in October 2022: PubMed, Scopus, Web of Science. All the clinical studies which investigated the outcomes of MPFL reconstruction using autografts in children and adolescents with recurrent patellofemoral instability were accessed. Only studies which included patients younger than 18 years were considered. Techniques, case reports, guidelines, comments, editorials, letters, protocols, reviews, and meta-analyses were excluded. Studies which included patients with congenital or acute patellofemoral instability were not eligible, nor were those which focused exclusively on hyperlaxity. Results: Data from 477 patients (510 procedures) were retrieved. Of the patients, 41% (196 of 477) were women. The mean length of follow-up was 33.7 ± 28.8 months. The mean age of the patients was 14.6 ± 1.6 years. At the last follow-up, all PROMs of interest were statistically improved. The mean time to return to sport was 6.1 ± 1.1 months. Of the patients, 27% reduced their level of activity after surgical stabilization. A total of 87% of patients returned to practice sport. A total of 5% (26 of 477) and 2% (9 of 363) of patients experienced further dislocations and subluxations, respectively, during the follow-up period. Moreover, 4% (16 of 403) of patients underwent a further surgical procedure for patellofemoral instability within the follow-up period. Conclusion: MPFL reconstruction using autografts is effective in children and adolescents with recurrent patellofemoral instability.
Impact of New-Type Agricultural Cooperatives on Profitability of Rice Farms: Evidence from Vietnam’s Mekong River Delta
Authors: Tran Nhan Quoc , Ngo Thong Van , Nguyen Nay Van , Duong Thanh Ngoc , Nguyen Can Duy , Quach Tu Duong , Le De Van
Published: December 2022

The major goal of this study is to examine the impact of agricultural cooperative membership on the net return on production cost (ROC). The data used for the study were gathered from 233 rice farmers in the central Mekong River Delta’s province of Hau Giang, Vietnam. Propensity score matching and two-step Heckman selection models were employed to account for possible selection bias stemming from systematically different observable and unobservable attributes between cooperative members and non-members. The findings reveal that cooperative membership has a positive and statistically significant impact on rice farmers’ ROC, suggesting that agricultural cooperatives could help increase the profitability of rice farms.
Proteolysis Degree of Protein Corona Affect Ultrasound-Induced Sublethal Effects on Saccharomyces cerevisiae: Transcriptomics Analysis and Adaptive Regulation of Membrane Homeostasis
Authors: Zheng Zi-Yi , Feng Chao-Hua , Xie Guo , Liu Wen-Li , Zhu Xiao-Lei
Published: December 2022

Protein corona (PC) adsorbed on the surface of nanoparticles brings new research perspectives on the interaction between nanoparticles and fermentative microorganisms. Herein, the proteolysis of wheat PC adsorbed on a nano-Se surface using cell-free protease extract from S. cerevisiae was conducted. The proteolysis caused monotonic changes of ζ-potentials and surface hydrophobicity of PC. Notably, the innermost PC layer was difficult to be proteolyzed. Furthermore, when S. cerevisiae was stimulated by ultrasound + 0.1 mg/mL nano-Se@PC, the proportion of lethal and sublethal injured cells increased as a function of the proteolysis time of PC. The transcriptomics analysis revealed that 34 differentially expressed genes which varied monotonically were related to the plasma membrane, fatty acid metabolism, glycerolipid metabolism, etc. Significant declines in the membrane potential and proton motive force disruption of membrane were found with the prolonged proteolysis time; meanwhile, higher membrane permeability, membrane oxidative stress levels, membrane lipid fluidity, and micro-viscosity were triggered.
Visualizing the Distribution of Lipids in Peanut Seeds by MALDI Mass Spectrometric Imaging
Authors: Wang Xin , Chen Yuning , Liu Yue , Ouyang Lei , Yao Ruonan , Wang Zhihui , Kang Yanping , Yan Liying , Huai Dongxin , Jiang Huifang , Lei Yong , Liao Boshou
Published: December 2022

Peanut (also called groundnut, Arachis hypogaea L.) seeds are used for producing edible oils and functional foods, and offer a rich source of lipids, proteins and carbohydrates. However, the location of these metabolites has not yet been firmly established. In the present study, the matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) technique was applied to investigate spatial distribution of lipids and other key components in seeds of three peanut cultivars (ZH9, KQBH, HP). A total of 103 metabolites, including 34 lipid compounds, were putatively identified by MALDI-MSI. The abundance and spatial distribution of glycerolipids (GLs) and glycerophospholipids (GPs) were compared among the three peanut cultivars. All the identified lysophosphatidylcholine (LPC), phosphatidylethanolamine (PE) and phosphatidylcholines (PCs) were distributed mainly in the inner part of seeds. The visualization of phosphatidic acids (PAs) and triacylglycerols (TGs) revealed a dramatic metabolic heterogeneity between the different tissues making up the seed. The non-homogeneous spatial distribution of metabolites appeared to be related to the different functions of particular tissue regions. These results indicated that MALDI-MSI could be useful for investigating the lipids of foodstuffs from a spatial perspective. The present study may contribute to the development of oil crops with higher oil yields, and to improvement of food processing.
Maternal Diet Quality and the Health Status of Newborns
Authors: Pavlikova Jitka , Ambroz Antonin , Honkova Katerina , Chvojkova Irena , Sram Radim J. , Rossner Pavel , Topinka Jan , Gramblicka Tomas , Parizek Ondrej , Parizkova Denisa , Schmuczerova Jana , Pulkrabova Jana , Rossnerova Andrea
Published: December 2022

The maternal diet during pregnancy affects neonatal health status. The objective of this study was to assess the nutritional quality of the maternal diet, and its contamination by persistent organic pollutants (POPs), in pregnant women living in two areas of the Czech Republic with different levels of air pollution, and subsequently to assess the relationship of these two factors with birth weight and neonatal oxidative stress. To determine the level of oxidative stress, 8-isoprostane concentrations in umbilical cord plasma were measured. The overall nutritional quality of the maternal diet was not optimal. Of the nutritional factors, protein intake proved to be the most significant showing a positive relationship with birth weight, and a negative relationship with the oxidative stress of newborns. Dietary contamination by persistent organic pollutants was low and showed no statistically significant relationship with birth weight. Only one of the 67 analyzed POPs, namely the insecticide dichlorodiphenyltrichloroethane (DDT), showed a statistically significant positive relationship with the level of neonatal oxidative stress.
Cold Climate during Bud Break and Flowering and Excessive Nutrient Inputs Limit Apple Yields in Hebei Province, China
Authors: Duan Zhiping , Zheng Chengjuan , Zhao Shuaixiang , Feyissa Tesema , Merga Tefera , Jiang Yuanmao , Zhang Weifeng
Published: December 2022

Apples have become a major source of income for smallholder farmers in Bohai Bay. However, the annual productivity of apples in the area is relatively low and the interannual yield gap varies drastically. Identifying the apple yield gap and interannual production constraints can potentially promote the sustainable development of apple production. Based on track monitoring data of 45 smallholder farmers from 2016 to 2018, the yield gap and constraint factors were determined by adopting boundary analysis methodology. The results showed that the yield potential of apples during 2016–2018 was 75, 108, and 87 t ha−1, and actual yields were 36.8, 52.3, and 35.2 t ha−1, respectively. The explainable yield gaps were 40.5, 56.9, and 55.1 t ha−1. Soil, management, and climatic factors limit apple yield improvement. Among these, low temperatures during the bud break and flowering periods can induce yield losses. Soil nutrient content and fertilizer management are also important limiting factors that have polynomial relationships with yield. Too much fertilizer and high levels of nutrients in the soil have already caused yield losses in some fields. Sound scientific guidance to help farmers adopt reasonable management techniques adapted to climate change is necessary to close the yield gap.
Kaffir Lime Essential Oil Variation in the Last Fifty Years: A Meta-Analysis of Plant Origins, Plant Parts and Extraction Methods
Authors: Budiarto Rahmat , Sholikin Mohammad Miftakhus
Published: December 2022

Kaffir lime has been widely researched for use as an essential oil, alongside its main function as an Asian spice, due to the needs of numerous industries. A meta-analysis was used to summarize the variation in yield and main components of kaffir lime essential oils in response to differences in plant origins, plant parts, and extraction methods during the last fifty years. A database was constructed from 85 data items from 36 single studies, prepared by following PRISMA-P. The result showed no significant effect of extraction method on variation in oil yield and main components. In contrast, after integrating numerous single studies under a statistical approach, some interesting facts emerged, such as (i) plant origin significantly affected the citronellol, i.e., subtropical conditions were more favorable than tropical ones; (ii) caryophyllene was found in all countries, from subtropical America and tropical Asia to tropical East Africa; (iii) the richest parts of citronellal, citronellol, citronellyl acetate, and sabinene were leaves, blossoms, twigs, and fruit peels, respectively; and (iv) due to significant interaction of plant origin and plant parts, a very challenging issue in boosting oil yield could be answered by formulating location-specific and organ-specific culture practices. This study had succeeded in providing further research directions.
Full-Length Transcriptome and Transcriptome Sequencing Unveil Potential Mechanisms of Brassinosteroid-Induced Flowering Delay in Tree Peony
Authors: Zhang Lin , Song Chengwei , Guo Lili , Guo Dalong , Xue Xian , Wang Huafang , Hou Xiaogai
Published: December 2022

Tree peony (Paoenia ostii) is a famous Chinese traditional flower well-known in many countries of the world. However, the short and concentrated flowering period of tree peony greatly affects the ornamental and economic value of the flowers. Exogenous brassinosteroid (BR) treatment can delay the flowering period of ostii T. Hong et J. X. Zhang var. lishizhenenii B. A. Shen for 3 days, but the underlying regulatory mechanism remains elusive. Here, full-length transcriptome and transcriptome sequencing were used to mine key genes related to BR-induced delayed flowering in tree peony. The transcriptome sequencing of the petals yielded 21.27 G clean data and 62,229 isoforms. Among them, 58,218 isoforms were annotated in NR, NT, SwissProt, KEGG, KOG, InterPro and GO databases. GO and KEGG analyses showed that 2460 DEGs were related to delayed flowering in response to BR. Additionally, a total of seven genes affecting flowering were annotated from 11 isoforms, which responded to BR through three pathways to delay the flowering of P. ostii var. lishizhenii. BR treatment increased the expression of BRASSINOSTEROID-SIGNALING KINASE3 (BSK3), potentially by promoting BRI1 ASSOCIATED KINASE RECEPTOR 1 (BAK1). Moreover, BR treatment suppressed the expression of SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE 1 (SPL1), possibly to inhibit the expressions of downstream WRKY genes and APETALA 2 (AP2). Meanwhile, BR treatment promoted the expression of DELLA, which might inhibit the expressions of CONSTANS (CO) and SPL. These results suggest a theoretical basis for further analyses of the molecular mechanism of flowering regulation in tree peony.
Layered Gadolinium-Europium-Terbium Hydroxides Sensitised with 4-Sulfobenzoate as All Solid-State Luminescent Thermometers
Authors: Rodina Anfisa A. , Yapryntsev Alexey D. , Abdusatorov Bakhodur A. , Belova Ekaterina V. , Baranchikov Alexander E. , Ivanov Vladimir K.
Published: December 2022

Ternary layered gadolinium-europium-terbium basic chlorides were synthesised using a facile hydrothermal-microwave technique. A continuous series of solid solutions was obtained in a full range of rare earth concentrations. To sensitise the luminescence of Eu3+ and Tb3+, a 4-sulfobenzoate anion was intercalated in the ternary layered rare earth hydroxides using one of two methods—a high-temperature ion exchange or a single-stage synthesis. The luminescent colour of the materials was governed by the gadolinium content: at low and medium gadolinium concentrations (0–70%), layered Gd-Eu-Tb basic sulfobenzoate exhibited a bright red europium luminescence; at high gadolinium content (70–90%), a bright green terbium luminescence was observed. The colour coordinates of layered Gd-Eu-Tb basic sulfobenzoate luminescence depended on the temperature in the physiological range (20–50 °C). The relative thermal sensitivity of the obtained materials was up to 2.9%·K−1.
Investigation and Numerical Simulation of the Acoustic Target Strength of the Underwater Submarine Vehicle
Authors: Sathish Kaveripakam , Anbazhagan Rajesh , Venkata Ravikumar Chinthaginjala , Arena Fabio , Pau Giovanni
Published: December 2022

Modern weapon systems’ survival hinges on their detection capabilities more than anything else. In the active sonar equation, the acoustic target strength is crucial. Under the assumption of plane wave propagation, the standard target strength equation is used to forecast the reradiated intensity for the far field. The ability of a submarine to remain unnoticed while on patrol or accomplishing a mission is its primary defense. Sonar, sometimes known as sound navigation ranging, is a popular method for locating submarines. This is because saltwater effectively absorbs radio frequencies. Sonar technology is used in more than just the commercial fishing business; it is also used in undersea research. The submarine’s designers consider the reflection of acoustic waves to minimize the amount of space required for such reflections. The Target Strength (TS) metric is used to assess the sonar objects’ size. This manuscript explains and demystifies the Benchmark Target Echo Strength Simulation (BeTTSi) benchmark submarine’s TS analysis. This model’s Pressure Acoustic-Boundary Element Model (PA-BEM) interface has been stabilized, and the model itself is pretty huge acoustically.
The Extraction Method of Navigation Line for Cuttage and Film Covering Multi-Functional Machine for Low Tunnels
Authors: Li Yumeng , Zhu Yanjun , Li Shuangshuang , Liu Ping
Published: December 2022

Aiming at the problem of low intelligence in the automatic navigation of the cuttage and film covering multi-functional machine for low tunnels, this study proposed a navigation line extraction method based on the improved YOLOv5s model, which can achieve the accurate extraction of navigation lines based on two planting methods of seedling transplanting and direct seeding. Firstly, we pre-processed the acquired images using inverse perspective transformation. Next, the Coordinate Attention and Ghost modules were applied to improve the YOLOv5s architecture, increasing the detection accuracy and speed of field targets. Finally, we extracted the feature points and fit the navigation lines based on the shape features of the targets using the geometric method. The experimental results showed that, compared with other algorithms, the accuracy of the proposed algorithm could reach more than 96%, the accuracy of navigation line extraction reached 98%, and the average detection time was 51 ms. The proposed method was robust and universal, and it can provide reliable navigation paths for the cuttage and film covering multi-functional machine.
Optimization of Shear Bonds of the Grouted Joints of Offshore Wind Turbine Tower Based on Plastic Damage Model
Authors: Zhou Linqing , Sun Weiyuan , Cao Yu , He Chaonan , Ma Xinwei
Published: December 2022

In recent years, offshore wind power has been developing rapidly, and single piles are among the commonly used foundations for wind turbines. Presently, experimental studies of the grouted connections of pile foundations are limited to the study of scaler models. Numerical simulations are more suitable for the mechanical analysis of the full-size structure instead of experimental ones. In numerical simulations, the linear elasticity model is widely adopted, but the plastic damage is studied scarcely. So, shear bond parameter research concerning grouted joints needs to be supplemented. In this paper, a bilinear random-motion reinforcement model based on the classical metal plasticity theory is adopted for steel, and the model for the grouting material is based on the Sidiroff energy equivalence principle. The plastic damage model for the grouted connecting section is established; the stresses and deformation distribution of the steel pipes and grout in the connecting section are analyzed using the changed shear bond parameters. The results show that the rectangular and triangular shear bonds are more reasonable than the semicircular shear bond transfer. Increasing the height of the shear bond may reduce the maximum stress and the maximum vertical displacement of the grout, and the shear bond width change has less influence on the joint bond stress and displacement.
Effects of Acute High-Temperature Stress on Physical Responses of Yellowfin Tuna (Thunnus albacares)
Authors: Liu Hongyan , Fu Zhengyi , Yu Gang , Ma Zhenhua , Zong Humin
Published: December 2022

To understand the physiological reactions of juvenile yellowfin tuna (Thunnus albacares) under acute high-temperature stress, this study measured the changes in biochemical indexes of serum, liver, gill, and muscle of yellowfin tuna under acute high-temperature stress (HT, 34 °C) and a control group (28 °C) for 0 h and 6 h, 24 h and 48 h. The rising speed of water temperature in the HT group was 2 °C/h and the timing started when the temperature reached 34 °C. In the HT group, there was no significant difference between the four adjacent times in cortisol and lactic acid concentration. Serum triglyceride, cholesterol, and alkaline phosphatase concentration were significantly different from the four adjacent times. The superoxide dismutase (SOD) activity in the liver and gills increased at 6 h and 24 h, and the gills and liver had antioxidant reactions in a short time. The malondialdehyde (MDA) concentration in the gills changed significantly at 6 h, while that in the liver did not change significantly. The gills were more sensitive to temperature stress than the liver and muscle. Acute high-temperature stress affected yellowfin tuna’s antioxidant enzymes and metabolic indexes, resulting negative trend in physiological indexes, indicating that yellowfin tuna juveniles are susceptible to elevated temperature.
Influence of Ethanol on Porous Anodic Alumina Growth in Etidronic Acid Solutions at Various Temperatures
Authors: Kwiatkowska Małgorzata , Siemiaszko Dariusz , Norek Małgorzata
Published: December 2022

Etidronic acid, used in aluminum anodization, has a great potential for the fabrication of porous anodic alumina (PAA) with large cell sizes (>540 nm). PAAs are particularly suited to applications in optics and photonics where large-scale periodicity corresponding to visible or infrared light is needed. Additionally, such PAAs should be characterized by long-range pore ordering. However, to obtain regular pore arrangement in an etidronic electrolyte, the anodization should be performed at high electric fields using relatively high temperatures, which makes the process challenging in terms of its stability. To stabilize the process, the electrolyte can be modified with ethanol. In this work, the impact of ethanol on pore geometry and a level of pore ordering is systematically analyzed. It is shown that the additive tends to reduce pore ordering. Moreover, by changing the anodizing temperature and the amount of ethanol, it is possible to tune the porosity of the PAA template. At 20 °C, porosity drops from 14% in PAA grown in a pure water-based electrolyte to ca. 8% in PAA fabricated in the 1:3 v/v EtOH:H2O electrolyte. The larger PAA thickness obtained for the same charge density strongly suggests that PAA formation efficiency increases in the 1:3 v/v EtOH:H2O mixture.