bluuebunny/crossref_metadata_2025 huggingface dataset:
Beyond human expertise: the promise and limitations of ChatGPT in suicide risk assessment
Authors: Elyoseph Zohar , Levkovich Inbar
Published: August 2023

ChatGPT, an artificial intelligence language model developed by OpenAI, holds the potential for contributing to the field of mental health. Nevertheless, although ChatGPT theoretically shows promise, its clinical abilities in suicide prevention, a significant mental health concern, have yet to be demonstrated. To address this knowledge gap, this study aims to compare ChatGPT’s assessments of mental health indicators to those of mental health professionals in a hypothetical case study that focuses on suicide risk assessment. Specifically, ChatGPT was asked to evaluate a text vignette describing a hypothetical patient with varying levels of perceived burdensomeness and thwarted belongingness. The ChatGPT assessments were compared to the norms of mental health professionals. The results indicated that ChatGPT rated the risk of suicide attempts lower than did the mental health professionals in all conditions. Furthermore, ChatGPT rated mental resilience lower than the norms in most conditions. These results imply that gatekeepers, patients or even mental health professionals who rely on ChatGPT for evaluating suicidal risk or as a complementary tool to improve decision-making may receive an inaccurate assessment that underestimates the actual suicide risk.
Case report: specific phobia of vaginal penetration in a pregnant patient
Authors: Geng Wenqi , Cao Jinya , Jin Li , Wei Jing
Published: August 2023

Specific phobia is frequently unrecognized or untreated unless it causes significant impairment. In this report, we documented a rare case of a pregnant patient who had a specific fear related to vaginal penetration. Due to abnormal fetal cardiac development in the second trimester, the patient was admitted for termination of pregnancy. The patient’s persistent request for surgical termination via cesarean delivery prompted the obstetrician to seek psychiatric consultation for tokophobia, a labor- and childbirth-related phobia. The consulting psychiatrist discovered that the patient had developed a significant fear of vaginal penetration during adolescence. Throughout the extended period of this specific phobia, the patient established a range of avoidance strategies. Had it not been for the unforeseen need for abortion, her phobia may not have been identified. Psychoeducation on specific phobias, exposure therapy, muscle relaxation techniques, and the administration of anxiolytics were implemented. The pregnancy was terminated through a vaginal labor induction procedure 2 days later. Collaboration across disciplines is necessary to support a thorough assessment of obstetric patients who express hesitancy toward vaginal delivery.
Case report: Occupational poisoning incident from a leak of chloroacetyl chloride in Jinan, Shandong, China
Authors: Guo Lanlan , Zhang Xiangxing , Zhou Zhiqiang , Shi Mengdi , Jian Xiangdong , Dong Laidong
Published: August 2023

Chloroacetyl chloride is a potent acylation agent that decomposes violently in water to produce chloroacetic acid and irritant hydrogen chloride. It and its decomposition products are corrosive to the eyes, skin, and respiratory system and can cause multiple organ failure. Herein, we report cases of poisoning by chloroacetyl chloride and its decomposition products in the skin and respiratory system. After exposure, one patient developed vomiting, irritability, coma, hypoxemia, hypotension, acidosis, and hypokalemia. Another patient developed bronchiolitis, pneumonia, and decreased vision. One patient died and two recovered. Chloroacetyl chloride and its decomposition products are corrosive and can damage multiple organs after absorption through the skin and respiratory tract, leading to severe heart failure. Cardiogenic shock may be the primary cause of early mortality.
The acceptability of homebased exercise snacking and Tai-chi snacking amongst high and low function UK and Taiwanese older adults
Authors: Liang Ian Ju , Francombe-Webb Jessica , McGuigan Polly M. , Perkin Oliver J. , Thompson Dylan , Western Max J.
Published: August 2023

Introduction: Exercise “snacking” and Tai-chi ‘snacking’ protocols are designed to overcome typical barriers to older adults’ participation in muscle strength and balance exercise, using short bouts of home-based exercise. This study aimed to investigate the acceptability of homebred exercise- and Tai-chi snacking in British and Taiwanese older adults of high and low physical function.

Methods: Thirty-three British and Thirty Taiwanese older adults took part in semi-structured interviews, after trying 1-week of exercise- and Tai-chi snacking. The interview schedule and deductive framework analysis was based on the seven components of the Theoretical Framework of Acceptability (TFA). Differences between the Taiwanese and United Kingdom participants and those considered high versus low physical function were also analysed.

Results: Both snacking regimes were found to be convenient and easy to implement. Participants reported that no activity had to be given up, and considered the programmes would be beneficial to their physical and mental health. Interestingly, more UK-based participants preferred the elegant and relaxing movements of Tai-chi snacking, yet participants with low physical function experienced difficulties when mastering Tai-chi movements. A few high physical function participants perceived exercise snacking to be tedious.

Discussion: Overall, the snacking exercise was found to be acceptable and useful. Personal affective attitude and different cultural backgrounds may affect exercise participation. Nevertheless, it is important to consider individuals’ physical function when designing exercise regime. The findings indicate that making Tai-chi snacking easier to master initially, building in progression and adding some upper body movements in the exercise snacking may further enhance acceptability.
Research progress on the regulation of production traits by gastrointestinal microbiota in dairy cows
Authors: Liu Lily , Wu Peifu , Guo Aiwei , Yang Yajin , Chen Fenfen , Zhang Qin
Published: August 2023

The composition and abundance of microorganisms in the gastrointestinal tract of cows are complex and extensive, and they play a crucial role in regulating nutrient digestion, absorption, maintaining digestive tract stability, and promoting the production and health of the host. The fermentation carried out by these microorganisms in the gastrointestinal tract is fundamental to the health and productivity of cows. Rumen microorganisms produce the majority of enzymes required to break down feed substrates, such as cellulose, protein, lipids, and other plant materials, through fermentation. This process provides energy metabolism substrates that satisfy approximately 70% of the host’s energy requirements for physiological activities. Gut microorganisms primarily decompose cellulose that is difficult to digest in the rumen, thereby providing heat and energy to the hosts. Additionally, they have an impact on host health and productivity through their role in immune function. Understanding the composition and function of the cow gut microbiota can help regulate dairy cattle breeding traits and improve their health status. As a result, it has become a popular research topic in dairy cattle breeding. This article provides a review of the composition, structure, physiological characteristics, and physiological effects of the cow gut microbiota, serving as a theoretical foundation for future studies that aim to utilize the gut microbiota for dairy cattle breeding or improving production traits. It may also serve as a reference for research on gut microbiota of other ruminants.
Nonlinear Mixed-Effect Models to Describe Growth Curves of Pepper Fruits in Eight Cultivars Including Group Effects
Authors: Teixeira Filipe Ribeiro Formiga , Cecon Paulo Roberto , Suela Matheus Massariol , Nascimento Moysés
Published: August 2023

Evaluating the behavior of fruit width and length characters throughout the pepper crop cycle is essential for researchers in decision-making aimed at developing appropriate management techniques and harvesting fruits at proper growth stages. The Nonlinear Mixed-Effect Models (NLME) method is recommended to jointly model the residuals and the entire database, including group effects, to describe growth curves. This work compared four nonlinear equations (Gompertz, Logistic, Richards, and von Bertalanffy) by including groups (pepper and bell pepper) to describe the pepper genotypes’ length and width growth. Of the eight genotypes used, three were bell pepper, and five were pepper. For each, fruit length and width were measured in 10 periods. According to the fit-quality measures studied, the best model for adjusting the length of the fruit was the Richards (Radj.2=0.9960), while for the width, it was the Logistic (Radj.2=0.9957). The estimated random effects showed that for asymptotic length and time to the inflection point presented a correlation of 0.75, indicating a positive association between these traits. For width, however, this result was different: −0.02. NLME adjustment allowed efficient prediction of values and efficient characterization of the studied genotypes, proving to be an efficient method for longitudinal data.
Characterization of the Gut Microbiome in Healthy Dogs and Dogs with Diabetes Mellitus
Authors: Kwong Tsz Ching , Chau Eddie Chung Ting , Mak Mark Chi Ho , Choy Chi Tung , Chan Lee Tung , Pang Chun Keung , Zhou Junwei , Poon Phoebe Hoi Ching , Guan Yuqiong , Tsui Stephen Kwok Wing , Chan Shun Wan , Leung George Pak Heng , Tai William Chi Shing , Kwan Yiu Wa
Published: August 2023

With a close pathogenetic resemblance to human diabetes, canine Diabetes Mellitus, a chronic metabolic disease featuring abnormally high blood sugar levels, is increasing in prevalence worldwide. Unlike humans, canine glycemic control requires life-long insulin injections and dietary control in most cases, thereby jeopardizing diabetic dogs’ quality of life and increasing the difficulty of disease control. While many research studies have focused on elucidating the relationship between the canine gut microbiome and diseases, there is currently no research on the subject of diabetes mellitus in dogs. We hypothesized that the gut microbiome of canines with diabetes mellitus is different from that of healthy controls. Thus, we performed targeted 16S rRNA sequencing and comprehensive bioinformatic analysis to compare the gut microbiome profiles of 16 diabetic dogs with those of 32 healthy dogs. Clostridioides difficile, Phocaeicola plebeius, Lacrimispora indolis, and Butyricicoccus pullicaecorum were found to be enriched in diabetic dogs. A distinct shift towards carbohydrate degradation metabolic pathways was found to be differentially abundant in the diabetic subjects. Alteration of the co-occurrence network was also evident in the diabetic group. In conclusion, our study suggests that the gut microbial landscape differs in diabetic canines at the genera, species, functional, and network levels. These findings have significant implications for disease management, and thus warrant further research.
Impact of the Dog–Human Bond on Canine Social Evaluation: Attachment Predicts Preference toward Prosocial Actors
Authors: Richards Emily M. , Silver Zachary A. , Santos Laurie R.
Published: August 2023

Scholars have argued that social evaluation, the capacity to evaluate different potential social partners, is an important capacity not just for humans but for all cooperative species. Recent work has explored whether domesticated dogs share a human-like ability to evaluate others based on prosocial and antisocial actions toward third parties. To date, this work has shown mixed results, suggesting that individual differences may play a role in dogs’ capacity to evaluate others. In the present study, we test whether attachment—an individual difference that affects human social evaluation performance—can explain the mixed pattern of social evaluation results observed in dogs. We first tested dogs on a social evaluation task in which an experimenter either helped or refused to help the dog’s owner open a container. We then assessed dogs’ attachment strength using a subset of the C-BARQ. We found that attachment was a statistically significant predictor of dogs’ preference toward the prosocial actor but was not a predictor in antisocial or control conditions. This finding provides early evidence that attachment may drive positivity biases in dogs and that attachment might explain mixed results within canine social evaluation literature.
Chemical Composition and Functional Properties of Spray-Dried Animal Plasma and Its Contributions to Livestock and Pet Health: A Review
Authors: Kazimierska Katarzyna , Biel Wioletta
Published: August 2023

Spray-dried animal plasma (SDAP) is a functional ingredient derived from healthy animal blood, used as a nutritional additive in livestock and pet nutrition. SDAP is rich in macronutrients, micronutrients, and bioactive compounds such as immunoglobulins, albumin, growth factors, peptides, transferrin, and enzymes. This review focuses on the chemical composition of SDAP from porcine, bovine, and poultry sources, including protein quality and mineral profile. SDAP enhances performance and health in monogastric farm animals, aquaculture, and pets. It promotes growth rates and feed intake due to its high digestibility and superior amino acid profile compared to other protein sources. In pigs, SDAP’s positive effects stem from tissue-specific actions in the gastrointestinal tract, impacting digestion, immunity, and barrier function. For poultry, SDAP shows promise as a substitute for antibiotic growth promoters, particularly in chick starter diets. SDAP contains functional proteins that regulate immune response, enhance intestinal health, and aid in stress conditions. It is also used as a binder in pet food, providing high protein content and other desirable properties. SDAP meets the dietary requirements of carnivorous pets, appealing to owners seeking animal-derived protein sources. Additionally, SDAP may help prevent cognitive impairment in senior dogs and cats.
Airway Hyperresponsiveness, but Not Bronchoalveolar Inflammatory Cytokines Profiles, Is Modified at the Subclinical Onset of Severe Equine Asthma
Authors: Frippiat Thibault , Art Tatiana , Tosi Irene
Published: August 2023

Airway hyperresponsiveness (AHR) and inflammation are both observed in human and equine asthma. The aim of this study was to assess the timeline and relationship of both features at the subclinical onset of severe equine asthma (SEA). First, the repeatability of the pulmonary function test (PFT) using impulse oscillometry system, and the methacholine bronchoprovocation test (BPT) were assessed at a 1-day interval on six SEA horses in clinical remission and six control horses. Then, clinical and ancillary tests were performed before and after a 1-week low-dust environmental challenge, including weighted clinical score, respiratory endoscopy, bronchoalveolar fluid cytology, PFT, and BPT. Both PFT and BPT showed acceptable repeatability. No test allowed SEA horses in clinical remission to be distinguished from control, unlike in human patients. Because of the low-dust environment, no significant difference was observed in the results of clinical and conventional ancillary examinations after the challenge. However, SEA horses showed increased AHR after the environmental challenge. At that stage, no signs of inflammation or changes in pro-inflammatory cytokines profiles (quantification and gene expression) were observed, suggesting AHR is present at an earlier stage of equine asthma than airway inflammation. This feature indicates SEA could present in a different disease pathway than neutrophilic human asthma.
Application of Severe Weather Nowcasting to Case Studies in Air Traffic Management
Authors: Esbrí Laura , Rigo Tomeu , Llasat María Carmen , Biondi Riccardo , Federico Stefano , Gluchshenko Olga , Kerschbaum Markus , Lagasio Martina , Mazzarella Vincenzo , Milelli Massimo , Parodi Antonio , Realini Eugenio , Temme Marco-Michael
Published: August 2023

Effective and time-efficient aircraft assistance and guidance in severe weather environments remains a challenge for air traffic control. Air navigation service providers around the globe could greatly benefit from specific and adapted meteorological information for the controller position, helping to reduce the increased workload induced by adverse weather. The present work proposes a radar-based nowcasting algorithm providing compact meteorological information on convective weather near airports for introduction into the algorithms intended to assist in air-traffic management. The use of vertically integrated liquid density enables extremely rapid identification and short-term prediction of convective regions that should not be traversed by aircraft, which is an essential requirement for use in tactical controller support systems. The proposed tracking and nowcasting method facilitates the anticipation of the meteorological situation around an airport. Nowcasts of centroid locations of various approaching thunderstorms were compared with corresponding radar data, and centroid distances between nowcasted and observed storms were computed. The results were analyzed with Method for the Object-Based Evaluation from the Model Evaluation tools software (MET-10.0.1, Developmental Testbed Center, Boulder, CO, US) and later integrated into an assistance arrival manager software, showing the potential of this approach for automatic air traffic assistance in adverse weather scenarios.
Spatiotemporal Assessment and Correction of Gridded Precipitation Products in North Western Morocco
Authors: Ait Dhmane Latifa , Moustadraf Jalal , Rachdane Mariame , Saidi Mohamed Elmehdi , Benjmel Khalid , Amraoui Fouad , Ezzaouini Mohamed Abdellah , Ait Sliman Abdelaziz , Hadri Abdessamad
Published: August 2023

Accurate and spatially distributed precipitation data are fundamental to effective water resource management. In Morocco, as in other arid and semi-arid regions, precipitation exhibits significant spatial and temporal variability. Indeed, there is an intra- and inter-annual variability and the northwest is rainier than the rest of the country. In the Bouregreg watershed, this irregularity, along with a sparse gauge network, poses a major challenge for water resource management. In this context, remote sensing data could provide a viable alternative. This study aims precisely to evaluate the performance of four gridded daily precipitation products: three IMERG-V06 datasets (GPM-F, GPM-L, and GPM-E) and a reanalysis product (ERA5). The evaluation is conducted using 11 rain gauge stations over a 20-year period (2000–2020) on various temporal scales (daily, monthly, seasonal, and annual) using a pixel-to-point approach, employing different classification and regression metrics of machine learning. According to the findings, the GPM products showed high accuracy with a low margin of error in terms of bias, RMSE, and MAE. However, it was observed that ERA5 outperformed the GPM products in identifying spatial precipitation patterns and demonstrated a stronger correlation. The evaluation results also showed that the gridded precipitation products performed better during the summer months for seasonal assessment, with relatively lower accuracy and higher biases during rainy months. Furthermore, these gridded products showed excellent performance in capturing different precipitation intensities, with the highest accuracy observed for light rain. This is particularly important for arid and semi-arid regions where most precipitation falls under the low-intensity category. Although gridded precipitation estimates provide global coverage at high spatiotemporal resolutions, their accuracy is currently insufficient and would require improvement. To address this, we employed an artificial neural network (ANN) model for bias correction and enhancing raw precipitation estimates from the GPM-F product. The results indicated a slight increase in the correlation coefficient and a significant reduction in biases, RMSE, and MAE. Consequently, this research currently supports the applicability of GPM-F data in North Western Morocco.
Development and Validation of CXCR4 Nomogram-Based Immune Infiltration/Tumor Inflammation in Primary Glioblastoma
Authors: Jiang Guannan , Zheng Zong-Qing , Zhang Jie , Tian Zhichao , Li Xiang , Yu Zhengquan , Wang Zhong , You Wanchun , Chen Gang
Published: August 2023

Glioblastoma (GBM) is a highly malignant and aggressive tumor with poor prognosis. Therefore, the discovery of new prognostic molecular markers is of great significance for clinical prognosis. The CXC chemokine receptor (CXCR) members play a key regulatory role in many cancers. In this study, we explore the clinical value and application of the CXCR members in primary glioblastoma. Two GBM datasets from The Cancer Genome Atlas (TCGA) and The China Glioma Genome Atlas (CGGA) databases were used to explore the relationship between differential expression of CXCRs and GBM subtypes as well as immune infiltration. C-X-C motif chemokine receptor 4 (CXCR4) was screened as an independent prognostic factor, and a nomogram and risk prediction model were developed and tested in the CGGA database using the TCGA database. Receiver operating curve (ROC) and decision curve analysis (DCA) found good accuracy and net benefit of the models. The correlation of CXCR4 with immune infiltration and tumor was analyzed using CancerSEA and TIMER. In in vitro experiments, we found that CXCR4 was significantly overexpressed in glioblastoma and was closely related to the inflammatory response of U251/U87 cells. CXCR4 is an excellent independent prognostic factor for glioblastoma and positively correlates with tumor inflammation.
Tumor Budding Should Be in Oral Cavity Cancer Reporting: A Retrospective Cohort Study Based on Tumor Microenvironment
Authors: Tan Ayca , Taskin Toros
Published: August 2023

The utility of histological grading, which is useful in predicting prognosis in many tumors, is controversial for oral squamous cell carcinoma (OSCC). Therefore, new histopathological parameters should be added to histopathology reports of OSCCs. The study aimed to evaluate the parameters of worst invasion pattern (WPOI) and tumor budding in patients with OSCC, to compare them with other histopathological parameters, clinical data and overall survival, and to evaluate these results within the literature. A total of 73 OSCC cases with excisional biopsies were included in this study. WPOI, tumor budding, cell nest size, tumor-stroma ratio, stromal lymphocyte infiltration and stroma type, as well as classical histopathological parameters, were evaluated on hematoxylin-eosin-stained sections. Perineural invasion, lymph node metastases, advanced stage, presence of more than five buds and single cell invasion pattern in univariate survival analyses are characterized by a shortened overall survival time. While there was no significant difference between WPOI results and survival in the survival analysis, WPOI 5 was associated with more frequent lymph node metastasis and advanced stage at the time of diagnosis compared to WPOI 4. We concluded that tumor budding and single-cell invasion should be considered prognostic histopathologic parameters in OSCC.
Recent Progress in Modulation of WD40-Repeat Domain 5 Protein (WDR5): Inhibitors and Degraders
Authors: Gurung Raju , Om Darlami , Pun Rabin , Hyun Soonsil , Shin Dongyun
Published: August 2023

WD40-repeat (WDR) domain proteins play a crucial role in mediating protein–protein interactions that sustain oncogenesis in human cancers. One prominent example is the interaction between the transcription factor MYC and its chromatin co-factor, WD40-repeat domain protein 5 (WDR5), which is essential for oncogenic processes. The MYC family of proteins is frequently overexpressed in various cancers and has been validated as a promising target for anticancer therapies. The recruitment of MYC to chromatin is facilitated by WDR5, highlighting the significance of their interaction. Consequently, inhibiting the MYC–WDR5 interaction has been shown to induce the regression of malignant tumors, offering an alternative approach to targeting MYC in the development of anticancer drugs. WDR5 has two protein interaction sites, the “WDR5-binding motif” (WBM) site for MYC interaction and the histone methyltransferases SET1 recognition motif “WDR5-interacting” (WIN) site forming MLL complex. Significant efforts have been dedicated to the discovery of inhibitors that target the WDR5 protein. More recently, the successful application of targeted protein degradation technology has enabled the removal of WDR5. This breakthrough has opened up new avenues for inhibiting the interaction between WDR5 and the binding partners. In this review, we address the recent progress made in targeting WDR5 to inhibit MDR5–MYC and MDR5–MLL1 interactions, including its targeted protein degradation and their potential impact on anticancer drug discovery.
Reverse Transcription Can Critically Impact the Diagnostic Outcome of BCR::ABL1 Quantitative Real-Time RT-PCR
Authors: Spiess Birgit , Kleiner Helga , Tarnopolscaia Irina , Naumann Nicole , Fabarius Alice , Hofmann Wolf-Karsten , Saussele Susanne , Seifarth Wolfgang
Published: August 2023

Reverse transcriptases (RT) are essential tools in BCR::ABL1 fusion transcript monitoring in chronic myeloid leukemia (CML). The RT type and cDNA priming method may impair the stoichiometry of cDNA synthesis, thereby potentially introducing a bias in BCR::ABL1 qRT-PCR data. Using the Acrometrix™ BCR::ABL1 reference panel and 37 clinical specimens, we have comparatively investigated the performance of the RTs MLV and SuperScript IV with random hexamer vs. target-specific priming. Quantitative RT-PCR results identified the priming type and RT type as major factors for diagnostic data variation, mainly due to the different efficacies of processing BCR::ABL1 low-copy-numbers (<50) compared to GUSB or ABL1 high-copy targets. The impairment of SuperScript IV in processing low- and high-copy-number RNA targets equally was not reflected by the diagnostically relevant Log (BCR::ABL1/GUSB%) values. Therefore, the correct representation of housekeeping and BCR::ABL1 target genes should have priority when aiming at as high a number of housekeeping gene copies as possible. Our data suggest that for improving BCR::ABL1 assay sensitivity, increased RNA/cDNA amounts and the use of distinct RT/priming combinations are advantageous. However, for inter-laboratory harmonization, the proper conversion factor according to the CML international standard (IS) has to be reevaluated each time the grade of RT is changed.
Predicting Overall Survival for Patients with Malignant Mesothelioma Following Radiotherapy via Interpretable Machine Learning
Authors: Wang Zitian , Li Vincent R. , Chu Fang-I , Yu Victoria , Lee Alan , Low Daniel , Moghanaki Drew , Lee Percy , Qi X. Sharon
Published: August 2023

Purpose/Objectives: Malignant pleural mesothelioma (MPM) is a rare but aggressive cancer arising from the cells of the thoracic pleura with a poor prognosis. We aimed to develop a model, via interpretable machine learning (ML) methods, predicting overall survival for MPM following radiotherapy based on dosimetric metrics as well as patient characteristics. Materials/Methods: Sixty MPM (37 right, 23 left) patients treated on a Tomotherapy unit between 2013 and 2018 were retrospectively analyzed. All patients received 45 Gy (25 fractions). The multivariable Cox regression (Cox PH) model and Survival Support Vector Machine (sSVM) were applied to build predictive models of overall survival (OS) based on clinical, dosimetric, and combined variables. Results: Significant differences in dosimetric endpoints for critical structures, i.e., the lung, heart, liver, kidney, and stomach, were observed according to target laterality. The OS was found to be insignificantly different (p = 0.18) between MPM patients who tested left- and right-sided, with 1-year OS of 77.3% and 75.0%, respectively. With Cox PH regression, considering dosimetric variables for right-sided patients alone, an increase in PTV_Min, Total_Lung_PTV_Mean, Contra_Lung_Volume, Contra_Lung_V20, Esophagus_Mean, and Heart_Volume had a greater hazard to all-cause death, while an increase in Total_Lung_PTV_V20, Contra_Lung_V5, and Esophagus_Max had a lower hazard to all-cause death. Considering clinical variables alone, males and increases in N stage had greater hazard to all-cause death; considering both clinical and dosimetric variables, increases in N stage, PTV_Mean, PTV_Min, and esophagus_Mean had greater hazard to all-cause death, while increases in T stage and Heart_V30 had lower hazard to all-cause-death. In terms of C-index, the Cox PH model and sSVM performed similarly and fairly well when considering clinical and dosimetric variables independently or jointly. Conclusions: Clinical and dosimetric variables may predict the overall survival of mesothelioma patients, which could guide personalized treatment planning towards a better treatment response. The identified predictors and their impact on survival offered additional value for translational application in clinical practice.
Review of CO2 Adsorption Materials and Utilization Technology
Authors: Ren Furao , Liu Weijun
Published: August 2023

This article introduces the recent research status of CO2 adsorption materials and effective ways of CO2 resource utilization. Molecular sieves have the advantages of a large specific surface area, a wide pore size range, recyclability, and good chemical and thermal stability. Metal–organic frameworks have diverse structures and broad application prospects. The captured CO2 is converted into valuable chemicals such as acids, alcohols, hydrocarbons, and esters as raw materials. The rapid development of biomass energy utilization of CO2, with strong biological adaptability, high yield, low production cost, and low pollutant emissions, is a feasible method to reduce CO2 emissions. This article analyzes the current research status of CO2 capture, conversion into chemicals, biomass energy, and industrial utilization from the perspective of catalytic conversion.
Guiding Hepatic Differentiation of Pluripotent Stem Cells Using 3D Microfluidic Co-Cultures with Human Hepatocytes
Authors: Fattahi Pouria , de Hoyos-Vega Jose M. , Choi Jong Hoon , Duffy Caden D. , Gonzalez-Suarez Alan M. , Ishida Yuji , Nguyen Kianna M. , Gwon Kihak , Peterson Quinn P. , Saito Takeshi , Stybayeva Gulnaz , Revzin Alexander
Published: August 2023

Human pluripotent stem cells (hPSCs) are capable of unlimited proliferation and can undergo differentiation to give rise to cells and tissues of the three primary germ layers. While directing lineage selection of hPSCs has been an active area of research, improving the efficiency of differentiation remains an important objective. In this study, we describe a two-compartment microfluidic device for co-cultivation of adult human hepatocytes and stem cells. Both cell types were cultured in a 3D or spheroid format. Adult hepatocytes remained highly functional in the microfluidic device over the course of 4 weeks and served as a source of instructive paracrine cues to drive hepatic differentiation of stem cells cultured in the neighboring compartment. The differentiation of stem cells was more pronounced in microfluidic co-cultures compared to a standard hepatic differentiation protocol. In addition to improving stem cell differentiation outcomes, the microfluidic co-culture system described here may be used for parsing signals and mechanisms controlling hepatic cell fate.
The Impact of CC16 on Pulmonary Epithelial-Driven Host Responses during Mycoplasma pneumoniae Infection in Mouse Tracheal Epithelial Cells
Authors: Iannuzo Natalie , Dy Alane Blythe C. , Guerra Stefano , Langlais Paul R. , Ledford Julie G.
Published: August 2023

Club Cell Secretory Protein (CC16) plays many protective roles within the lung; however, the complete biological functions, especially regarding the pulmonary epithelium during infection, remain undefined. We have previously shown that CC16-deficient (CC16−/−) mouse tracheal epithelial cells (MTECs) have enhanced Mp burden compared to CC16-sufficient (WT) MTECs; therefore, in this study, we wanted to further define how the pulmonary epithelium responds to infection in the context of CC16 deficiency. Using mass spectrometry and quantitative proteomics to analyze proteins secreted apically from MTECs grown at an air–liquid interface, we investigated the protective effects that CC16 elicits within the pulmonary epithelium during Mycoplasma pneumoniae (Mp) infection. When challenged with Mp, WT MTECs have an overall reduction in apical protein secretion, whereas CC16−/− MTECs have increased apical protein secretion compared to their unchallenged controls. Following Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) assessment, many of the proteins upregulated from CC16−/− MTECS (unchallenged and during Mp infection) were related to airway remodeling, which were not observed by WT MTECs. These findings suggest that CC16 may be important in providing protection within the pulmonary epithelium during respiratory infection with Mp, which is the major causative agent of community-acquired pneumoniae.
The Effect of the Mixed Extract of Kalopanax pictus Nakai and Achyranthes japonica Nakai on the Improvement of Degenerative Osteoarthritis through Inflammation Inhibition in the Monosodium Iodoacetate-Induced Mouse Model
Authors: Lee Hak-Yong , Park Young-Mi , Hwang Hai-Min , Shin Dong-Yeop , Jeong Han-Na , Kim Jae-Gon , Park Hyo-Yeon , Kim Dae-Sung , Yoo Jin-Joo , Kim Myung-Sunny , Kim Min-Jung , Yang Hye-Jeong , Choi Soo-Cheol , Lee In-Ah
Published: August 2023

Osteoarthritis is a chronic inflammatory disease, and, due to the lack of fundamental treatment, the main objective is to alleviate pain and prevent cartilage damage. Kalopanax pictus Nakai and Achyranthes japonica Nakai are herbal plants known for their excellent anti-inflammatory properties. The objective of this study is to confirm the potential of a mixture extract of Kalopanax pictus Nakai and Achyranthes japonica Nakai as a functional raw material for improving osteoarthritis through anti-inflammatory effects in macrophages and MIA-induced arthritis experimental animals. In macrophages inflamed by lipopolysaccharide (LPS), treatment of Kalopanax pictus Nakai and Achyranthes japonica Nakai mixture inhibits NF-κB and mitogen-activated protein kinase (MAPK) activities, thereby inhibiting inflammatory cytokine tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6), inflammatory factors PGE2, MMP-2, and MMP-9, and nitric oxide (NO) was reduced. In addition, in an animal model of arthritis induced by MIA (monosodium iodoacetate), administration of Kalopanax pictus Nakai and Achyranthes japonica Nakai mixture reduced blood levels of inflammatory cytokines TNF-α and IL-6, inflammatory factors prostaglandin E2(PGE2), matrix metalloproteinase-2(MMP-2), and NO. Through these anti-inflammatory effects, MIA-induced pain reduction (recovery of clinical index, increase in weight bearing, and increase in area and width of the foot), recovery of meniscus damage, loss of cartilage tissue or inflammatory cells in tissue infiltration reduction, and recovery of the proteglycan layer were confirmed. Therefore, it is considered that Kalopanax pictus Nakai and Achyranthes japonica Nakai mixture has the potential as a functional raw material that promotes joint health.
The Influence of Tempering Temperature on Retained Austenite and Ductility–Toughness of a High-Strength Low-Carbon Alloyed Steel
Authors: Sun Lirong , Wan Jiafeng , Zhang Jiqing , Wang Feng , Yuan Guo , Wang Guodong
Published: August 2023

High-strength alloyed steel has been widely used in engineering equipment because of its exceptional strength and toughness, particularly at low temperatures. However, the performance of high-strength alloy steel has not been fully developed, and it is necessary to further optimize the microstructure and mechanical properties. Therefore, the focus of this study is on the phase transition and corresponding mechanical properties of high-strength low-carbon alloyed steels. Three experimental steels were austenitized at 900 °C for 1 h, followed by water quenching, and were then tempered at 570, 600, and 630 °C. They were denoted as QT570, QIT600, and QIT630, respectively. The results show that appropriate intercritical tempering of QIT600 steel significantly increases the proportion of retained austenite and promotes VC precipitation within tempered martensite in comparison to QT570 and QIT630 steels. The enrichment of multiple alloys improved the thermal stability of retained austenite, which was further demonstrated with low-temperature insulation tests. Meanwhile, QIT600 steel with 18 vol.% of retained austenite achieved a superior yield strength of 1025 MPa, an elongation of 21%, and a cryogenic impact energy of 1.25 MJ/m2. The plasticity induced by the transformation of the retained austenite significantly enhanced the strain-hardening rate and postponed necking, thereby increasing elongation. The retained austenite enhanced cryogenic toughness by significantly arresting crack growth and increasing the ability of plastic deformation.
The First Mitochondrial Genome of an Odonata Endemic to South America, Chalcopteryx rutilans (Rambur, 1842) (Odonata: Polythoridae), and Its Implications for the Phylogeny of the Zygoptera
Authors: Juen Leandro , Koroiva Ricardo , Geraldo de Carvalho Fernando , Mendoza-Penagos Cristian Camilo , Brito Joás da Silva , Calvão Lenize Batista , Ferreira Victor Rennan Santos , Ribeiro-dos-Santos Ândrea , Silva Caio S. , Guerreiro Sávio , Cavalcante Giovanna C. , Magalhães Leandro , Souza Jorge E. S. de , Gomes Daniel H. F. , Montag Luciano Fogaça de Assis , Michelan Thaisa S. , Ligeiro Raphael
Published: August 2023

Chalcopteryx rutilans Rambur, 1842 (Polythoridae, Odonata) is a species widely distributed in central Amazonia. Due to its sensitivity to environmental changes, it is a bioindicator species used to evaluate the environmental conditions of streams in federally protected areas. By sequencing C. rutilans mitogenome, we report the first whole mitogenome from the Polythoridae family and the first from an Odonata species endemic to South America. The entire mitogenome has 15,653 bp and contains 13 protein-coding, 22 tRNA, and two rRNA genes. The nucleotide composition of the mitogenome is 42.7%, T: 25.5%, C: 19.4%, and G: 12.4%. The AT and GC skews of the mitogenome sequence were 0.249 and −0.220, respectively. C. rutilans was recovered as a sister to Pseudolestes mirabilis Kirby, 1900 (Pseudolestidae), demonstrating the absence of mitogenomes of species from multiple families in the current literature. Mitogenome data from this study will provide useful information for further studies on the phylogeny and conservation of Polythoridae.
Design, Modeling, and Control of Rotating and Linear Electric Machines for Automotive Applications
Authors: Amara Yacine
Published: August 2023

The automotive industry is one of the main employers in industrialized countries [...]
Optimization of LED-Based Solar Simulators for Cadmium Telluride and Microcrystalline Silicon Solar Cells
Authors: Vosylius Žygimantas , Novičkovas Algirdas , Tamošiūnas Vincas
Published: August 2023

Solar simulators are instruments used for controllable measurements of the properties of solar cells in indoor environments. The purpose of this paper is to examine the peculiarities of the photoresponses of CdTe/CdSeTe and microcrystalline Si solar cells and to reveal the pathways to reduction of spectrum mismatch effects when using light-emitting diode (LED)-based or hybrid LED and halogen lamp-based solar simulators of an A+-class spectrum with a small number of sources. While only four different LED types are needed to achieve an A+-class spectrum under updated IEC 60904-9:2020 standard requirements, as demonstrated by our results, additional ultraviolet LEDs are necessary to reduce the spectrum mismatch. For hybrid solar simulator configurations, the combination of cool white LED arrays and halogen emitters can serve as a main light source. Optimized for both solar cell types, hybrid simulators have a lower spectral deviation and better spectrum coverage compared to LED-only simulators with the same number of distinct source types. In addition, our results predict lower spectral mismatch errors for optimized simulators when compared with conventional Xe lamp-based simulators.
Simulation and Control Design of a Midrange WPT Charging System for In-Flight Drones
Authors: Allama Oussama , Habaebi Mohamed Hadi , Khan Sheroz , Islam Md. Rafiqul , Alghaihab Abdullah
Published: August 2023

Drones, or unmanned aerial vehicles (UAVs), have emerged as an indispensable tool across numerous industries due to their remarkable versatility, efficiency, and capabilities. Notwithstanding all these traits, drones are still limited by battery life. In this paper, we propose a genuine in-flight charging method without landing. The charging system consists of three orthogonal coils, among which the receiving coil is connected to the drone. The development of the model for wireless dynamic charging systems is achieved by integrating the receiver trajectory and velocity in the model. Furthermore, the model is significantly enhanced by introducing the concept of the positioning mutual coupling function for the receiver trajectory; thus, it is possible to simulate a genuine continuous trajectory for UAVs and link it to the systems’ total input power consumption. The developed control algorithm can direct the magnetic field resultant to track the exact trajectory of the drone. The real-time simulation of the multiparameter discrete extremum-seeking control (ESC) algorithm on the (DSP) F28379D hardware shows that the input power is maximized up to 12 W in a response time of 2 ms for a drone-hovering velocity of 8 m/s without any feedback.
The Online Attention to Research in Legal Medicine: An Altmetric Study on the Most Discussed Articles on the Web
Authors: Garcovich Daniele , Zhou Wu Angel , Adobes Martin Milagros
Published: August 2023

Background: Online attention is a relevant research feature that can be related to its societal impact. Online attention in the field of legal medicine was explored as well as the relation between citation count and the Altmetric Attention Score (AAS). Methods: A search was performed, through the free Dimensions app, during September 2020, on the journals related to legal medicine listed in the 2019 edition of the Journal Citation Reports (JCR). The 200 published items with the highest AAS were retrieved and screened for bibliometric data. Articles with related citations were gathered from Web of Science (WOS), Scopus, and Dimensions. Results: Seventy-five percent of the articles were published by five of the selected journals. Twitter and news outlets were the most prevalent Altmetric resources. Additionally, 73.5% of the articles were published between 2013 and 2020. The most frequent topics were the ones related to toxicology and pharmacology, followed by criminalistics and law and bioethics. A poor correlation was found between the citations in WOS, Dimensions, and Scopus and the AAS. Conclusions: The online attention on legal medicine-related topics can be considered high on social media. The high number of news outlets is a distinctive feature of the most discussed articles in legal medicine. In this field of medicine, citation-based metrics combined with Altmetric can allow a broader evaluation of research findings but it should be underlined that, when measuring very different aspects of a published item, the scores in classic citation databases and Altmetric do not have a direct relation.
Children with Chronic Immune Thrombocytopenia Exhibit High Expression of Human Endogenous Retroviruses TRIM28 and SETDB1
Authors: Tovo Pier-Angelo , Galliano Ilaria , Parodi Emilia , Calvi Cristina , Gambarino Stefano , Licciardi Francesco , Dini Maddalena , Montanari Paola , Branca Margherita , Ramenghi Ugo , Bergallo Massimiliano
Published: August 2023

Chronic immune thrombocytopenia (CITP) is an autoimmune disease whose underlying biologic mechanisms remain elusive. Human endogenous retroviruses (HERVs) derive from ancestral infections and constitute about 8% of our genome. A wealth of clinical and experimental studies highlights their pivotal pathogenetic role in autoimmune diseases. Epigenetic mechanisms, such as those modulated by TRIM28 and SETDB1, are involved in HERV activation and regulation of immune response. We assessed, through a polymerase chain reaction real-time Taqman amplification assay, the transcription levels of pol genes of HERV-H, HERV-K, and HERV-W; env genes of Syncytin (SYN)1, SYN2, and HERV-W; as well as TRIM28 and SETDB1 in whole blood from 34 children with CITP and age-matched healthy controls (HC). The transcriptional levels of all HERV sequences, with the exception of HERV-W-env, were significantly enhanced in children with CITP as compared to HC. Patients on eltrombopag treatment exhibited lower expression of SYN1, SYN2, and HERV-W-env as compared to untreated patients. The mRNA concentrations of TRIM28 and SETDB1 were significantly higher and were positively correlated with those of HERVs in CITP patients. The over-expressions of HERVs and TRIM28/SETDB1 and their positive correlations in patients with CITP are suggestive clues of their contribution to the pathogenesis of the disease and support innovative interventions to inhibit HERV and TRIM28/SETDB1 expressions in patients unresponsive to standard therapies.
Total Mercury Content in the Tissues of Freshwater Chelonium (Podocnemis expansa) and a Human Health Risk Assessment for the Amazon Population in Brazil
Authors: Targino Fábio Júnior , Ribeiro Joanna Damazio de Nunes , Simões Julia Siqueira , Carneiro Carla Silva , Lazzarini Stella Maris , Souza Aline Ramos , Ferreira Micheli da Silva , Mano Sergio Borges , Mársico Eliane Teixeira
Published: August 2023

Researchers recognize the silent, negative and deleterious effects caused by mercury pollution in gold mining areas. Freshwater turtles are culturally part of the diet of riverside populations in the Amazon region and this area presents mercury (Hg) pollution issues mainly due to gold mining activities. Thus, this research aimed to evaluate the total mercury (THg) content in the different organs of Amazonian giant river turtle (Podocnemis expansa) and carry out a human health risk assessment associated with the consumption of these animals. This study was conducted in the Vila Balbina, municipality of Presidente Figueiredo, state of Amazonas, Brazil. Skin (n = 28), muscle (n = 19) and brain (n = 2) samples were analyzed by Atomic Absorption Spectrometry (TDA-AAS) and a DMA-80™ mercury analyzer was used for the total mercury determinations. The average values found for THg in the skin, muscle and brain samples were, respectively, 0.1045 mg·kg−1, 0.1092 mg·kg−1 and 0.0601 mg·kg−1. Thus, THg was observed even though the P. expansa were kept in captivity, possibly due to previous contamination by air, water and food. The Hazard Quotient (HQ) was calculated considering a 9.07 g·day−1 intake dose of P. expansa and the consumption of turtles once a week showed an HQ = 2.45, which may cause long-term injuries to human health. Although the muscle concentrations were below the maximum limit established by the World Health Organization (WHO) and Brazilian regulatory agencies, it is important to evaluate consumption factors such as amount ingested, frequency and animal gender, which may cause a potential risk to regular consumers due to mercury bioaccumulation. The WHO may consider various aspects in order to warn the Amazon population about the severity and silent hazard of this metal, especially due to the importance of this matrix in the region. This region urgently needs government actions to inhibit clandestine mining and to prevent future serious, chronic health problems of the entire population.