bluuebunny/crossref_metadata_2025 huggingface dataset:
From genomic to panomic predictions: An intuitive PANOMICs platform utilizing advanced Machine Learning algorithms
Authors: Schwarzerova Jana , Nohel Michal , Sitkova Radka , Skarda Jozef , Provaznik Valentine , Waldherr Steffen , Nägele Thomas , Chmelik Jiri , Walther Dirk , Weckwerth Wolfram
Published: October 2024

Advanced sequencing techniques have propelled molecular biology research into what is often termed the post-genomics era. Consequently, there is a heightened focus on unraveling the functional connections between individual genes and their ultimate phenotype, an area referred to as predictive genomics. To support this quest, we developed an intuitive platform, PANOMICS, that we believe substantially facilitates the statistical model building to pathway inference, with applications ranging from agriculture to the biomedical field.
PANOMICS, designed using Matlab2023b App Designer, allows for the uploading of genomics data, such as Single Nucleotide Polymorphisms (SNPs) information, metabolomics, other omics data, and phenotype information. The integration of different omics layers for the prediction of phenotypic outcomes promises to improve the accuracy and shifts the original genomic prediction, based solely on genomic inforamtion, to panomic prediction, that integrates multiple layers of molecular organization. PANOMICS facilitates data visualization and predictive analysis using various approaches and methods, such as linear, non-linear, and deep learning approaches. These analyses include statistical prediction models calculated based on Least Absolute Shrinkage and Selection Operator (LASSO), Ridge Regression (RR), Elastic Net Regression (ENR), Gaussian Process Regression (GPR), Support Vector Regression (SVR), Partial Least Squares (PLS) regression, and Random Forest (RF), as well as sophisticated Long Short-Term Memory (LSTM) and Convolutional Neural Network (CNN) models. Genome-Wide Association Studies (GWAS) with visualization based on an implementation of mixed linear models such as Ridge Regression with Best Linear Unbiased Prediction (rrBLUP) and Polygenic Risk Scores (PRS) are all put into practice within the app. The integration of a full range of advanced techniques within the application enhances its utility for researchers aiming to identify and interpret complex genetic interactions. This can lead to a more efficient use of Machine Learning (ML) algorithms in fields ranging from agriculture to medicine.
Polyfunctional immunity and transcriptional imprint associated with protection by chimeric live-attenuated Zika virus vaccine in non-human primates
Authors: Dallmeier Kai , Ma Ji , Malengier-Devlies Bert , Verstrepen Babs , Alpizar Yeranddy , Vercruysse Thomas , Javarappa Mahadesh , Sanchez-Felipe Lorena , Koopmans Gerrit , de Groot Natasja , Matthys Patrick , Neyts Johan , Verschoor Ernst , Coelmont Lotte , Thibaut Hendrik Jan , Weyenbergh Johan Van
Published: October 2024

Though generally mild in humans, Zika virus (ZIKV) frequently causes severe disease and congenital defects in children born to mothers infected during pregnancy (Musso et al. NEJM 2019 PMID: 31597021; Shan et al. Cell Host Microbe 2018 PMID: 30008291). Previously, chimeric candidate vaccine YF-ZIK was shown to protect mice against lethal ZIKV infection and congenital malformations after single-dose immunization (Kum et al. npj Vaccines 2018 PMID: 30564463; Kum et al. Emerg. Microbes Infect. 2020 PMID: 32116148). Here we demonstrate that YF-ZIK is safe, induces strong antiviral immunity, and protects rhesus macaques against vigorous experimental challenge. YF-ZIK triggered within 7-14 days after a single subcutaneous dose high levels of neutralizing antibodies, which could significantly be boosted by a second immunization 4 weeks later. Passively transferred immune serum protects AG129 mice from lethal ZIKV challenge, supporting neutralizing antibodies as major correlate of protection. Humoral immunity is complemented by ZIKV-specific cellular responses, characterized by a balanced Th1/Th2 cytokine production. Transcriptional analyses demonstrate the induction of a gene expression profile that is comparable to that of the original yellow fever YF17D vaccine, involving multiple pathways favoring polyvalent immunity. When YF-ZIK vaccinated macaques were challenged with a high intradermal dose of ZIKV, viral RNA remains undetectable in most animals. Immunity may be sterilizing immunity as no elevation of ZIKV neutralizing antibodies, nor seroconversion to anti-ZIKV NS1 antibodies was evident. Protection is associated with an activation of innate and adaptive immunity early after challenge. Systems analysis unveils B-cell receptor TNFRSF17 as predictor for strong antibody responses to YF-ZIK vaccination in common with YF17D (Gaucher et al. J. Exp. Med. 2008 PMID: 19047440; Querec et al. Nat. Immunol. 2009 PMID: 19029902; Fourati et al. Nat. Immunol. 2022 PMID: 36316476 ), along with GNAS and CD207 (Langerin) as novel biomarkers predicting clinical outcomes. The favorable preclinical safety, immunogenicity and efficacy of YF-ZIK justifies future evaluation in humans.
Collateral benefits of ivermectin MDA designed for malaria against headlice in Mopeia, Mozambique: a cluster randomised controlled trial
Authors: Furnival-Adams Joanna , Houana Amelia , Nicolas Patricia , Montaña Julia , Martinho Samuel , Casellas Aina , Mundaca Hansel , Mbanze Jenisse , Soares Arlindo , Imputiua Saimado , Ruiz-Castillo Paula , Ribes Marta , Sanz Almudena , Sale Mussa Mamudo , Macucha Antonio , Elobolobo Eldo , Vegove Vegovito , Mutepa Victor , Munguambe Humberto , Xerinda Aida , Materula Felisbela , Rabinovich Regina , Saute Francisco , Chaccour Carlos
Published: October 2024

Background
Headlice are prevalent worldwide, with a higher burden in rural, lower-middle income settings. They can cause intense itchiness, discomfort, and secondary bacterial infections with potentially serious consequences. Ivermectin is known to be efficacious against headlice, and is also currently being evaluated as a malaria vector control tool. In this study, we explored risk factors for headlice, and assessed the efficacy of ivermectin mass drug administration designed for malaria against headlice.
Methods
We conducted an open-label, assessor-blind, cluster-randomized controlled trial in Mopeia, Mozambique. A single dose of ivermectin was given monthly to eligible humans or humans and livestock (humans: 400 ug/kg, livestock: 1% injectable 200 ug/kg) in 3 consecutive months during the rainy season. The control group received albendazole (humans only). 39 clusters (13 per arm) were randomly selected for the nested assessment of headlice prevalence. 1,341 treated participants were followed up at, 1, 2 and 3 months and 382 untreated (ineligible) participants at 3 and 6 months after baseline. Headlice diagnosis was determined by examination of the scalp. Logistic regression was used to identify risk factors for headlice at baseline, and to estimate the treatment effect at each time point. This study is registered with ClinicalTrials.gov (NCT04966702).
Results
The baseline prevalence of headlice was 11%. Risk factors included living with a household member with head itch, being female, and living with poor water and sanitation facilities. The treated population receiving ivermectin had significantly lower odds of having headlice at 3 months compared to those receiving albendazole (aOR 0.22 95%CI 0.05–0.89). There was no indirect effect on headlice among ineligible children.
Conclusion
In a highly endemic setting, mass drug administration with ivermectin significantly reduces headlice infestation prevalence among those who receive the drug for three sequential months. The lack of effect among untreated, ineligible children implies that additional interventions would be needed to interrupt local transmission.
The instructor and students mentoring program: interference towards a complete student academic performance in Queen College in Human Resource Management class
Authors: Alehegn Derese
Published: October 2024

This study looks into how a mentorship programmer can improve a student's academic achievement in a classroom. In order to evaluate the efficacy of the programmed, the study uses a mixed-methodology approach that includes qualitative data collection techniques. The intervention entails students receiving mentorship from an experienced instructor with the goal of enhancing both academic performance and student confidence. The study's conclusions point to a beneficial impact on academic performance because programmed participants' academic outcomes significantly improved. Additionally, the mentoring program was effective in raising students' self-assurance when it came to presenting their work. A consistent method of tracking and evaluating student development was weekly formative attendance follow-up, which provided a trustworthy gauge of the intervention's success.
Effects of open-label placebos across outcomes and populations: An updated systematic review and meta-analysis of randomized controlled trials
Authors: Fendel Johannes C. , Tiersch Carl , Sölder Paul , Gaab Jens , Schmidt Stefan
Published: October 2024

This work synthesizes and updates findings from four previous systematic-reviews and meta-analyses on open-label placebos (OLPs). For the first time, it directly tests whether OLPs have different effects on self-report versus objective outcomes and on clinical versus non-clinical samples. We searched eight data-bases up to November 9, 2023, and included 58 randomized controlled trials (RCTs), compromising 61 separate comparisons. OLPs yielded a small positive effect across various health-related conditions (k = 61, n = 4569, SMD = 0.36, 95% CI = 0.26; 0.45, p < 0.0001; I² = 56%). The OLP effect differed between self-report outcomes (k = 55, n = 3171, SMD = 0.40) and objective outcomes (k = 14, n = 1176, SMD = 0.02, Q = 9.81, p < 0.01), and between clinical samples (k = 22, n = 1398, SMD = 0.48) and non-clinical samples (k = 39, n = 3,171, SMD = 0.29, Q = 4.69, p < 0.05). Neithter the level of suggestiveness nor the type of control moderated the effect. These findings confirm on a large dataset that OLPs are effective for self-report but not for objective outcomes. They also point to potential differences in effectiveness and mechanisms in comparsion to deceptive placebos.
CENP-A promotes cervical cancer progression through p53 pathway
Authors: Wang Yulin , Chen Shuang , Zhai Yuqing , Cao Wenjing , Zhang Zongliang , Tan Xueying , Yuan Jiangshui , Song Weiqing
Published: October 2024

Background: Centromere protein A (CENP-A) is a variant of histone H3 found in the centromeric chromatin, it plays a crucial role in chromosome segregation. The specific mechanisms by which CENP-A regulates cervical cancer (CC) progression have not been previously elucidated.
Methods: The Cancer Genome Atlas (TCGA) and Gene-Expression Omnibus（GEO）were used to analyze CENP-Aexpression in CC. Gene set enrichment analysis was constructed through GSEA. The expression of CENP-A in CC were tested using immunohistochemistry (IHC) staining. CC cells were transfected with LV- CENP-A-RNAi vector to decrease the CENP-A expression. Cell phenotype assays were used to detect the survival, proliferation and migration of cells. The cell apoptosis of CC cells was analyzed by flow cytometry assays.
Result: The expression of CENP-A was higher in cervical cancer tissues and cells compared with that in the adjacent paracancerous tissues. Gene enrichment analysis showed that the genes closely related to CENP-A were mainly related to the cell cycle, oocyte meiosis, pyrimidine metabolism, and the p53 signaling pathway in biological processes. The downregulation of CENP-A resulted in a reduction in the proliferation and migration of CC cells and an increase in cell apoptosis in vitro. In addition, p53, BAX and caspase3 were increased, whereas the expression of Bcl2 was decreased in the CENP-A-downregulated CC cells. After treatment with an antagonist of p53 (Pifithrin-α), the phenotype of CC cells proliferation inhibition was recovered.
Conclusion: CENP-A overexpression may be regulated to poor prognosis in patients with cervical cancer. These results suggest that CENP-A may associated with cell apoptosis and proliferation in cervical cancer cells and this may be mediated by p53 signal pathway. CENP-A may be used as a predictor of diagnosis,progression and prognosis in patients with cervical cancer.
Genetic Evidence Supports the Therapeutic Potential of Immune Checkpoint PD-L2 in the Development of Keloid
Authors: Wang Ben , Deng Yiwen , Ran Zhujie , Pan Bo , Jiang Haiyue
Published: October 2024

Keloids are pathological scars that extend beyond the original skin injury, resembling tumor-like growth, with unclear etiology and challenging treatment. This study aims to determine whether there is a causal link between the immune checkpoint PD-L2 and keloid risk, assessing immune checkpoints as potential therapeutic targets. A Mendelian randomization analysis was conducted using genome-wide association study summary statistics. PD-L2 expression levels were analyzed in Asian and European cohorts to explore their association with keloid risk. Two clinical cohorts with different ancestries were used to investigate association between PD-L2 expression and the risk of keloids. The Asian cohort included 178,726 samples (1055 cases), while the European cohort included 481,912 samples (668 cases). Our findings indicate that higher levels of PD-L2 expression in plasma proteome are associated with an increased risk of keloids. This conclusion holds true in both European and Asian large-scale cohorts, with OR (Asia) values of 1.25 (1.04-1.51), P_IVW=0.02, and OR (Europe) of 1.24 (1.07-1.43), P_IVW=0.004. This study reveals for the first time a causal relationship between the expression levels of the immune checkpoint PD-L2 and the risk of keloids. This provides a new perspective for understanding the pathogenesis of keloid and for developing new treatment and prevention strategies.
GPC2 provides prognostic value in pan-pediatric cancers and is associated with MYCN amplification in neuroblastoma：Bioinformatics Analysis and Validation
Authors: Xu Yanfeng , Zhou Ziang , Dong Yanqun , Wang Guanyun , Zheng Lingling , Lu Xia , Li Siqi , Zhang Mingyu , Gong Jianhua , Yang Jigang
Published: October 2024

Background
Glypican-2 (GPC2), a member of the GPC gene family, primarily functions in developing neural and thyroid cancer tissues, exerting influence on protein transduction, cellular proliferation and differentiation, as well as oncogenic signatures. GPC2 exhibits significant overexpression in the majority of neuroblastoma (NB) samples while remaining nearly undetectable in normal pediatric tissue samples.
Methods
Overall survival (OS) was employed as a key parameter to investigate the correlation between GPC2 expression and pan-pediatric cancers. To assess the association between GPC2 expression and clinical parameters of NB, box plots followed by t-tests were utilized. Protein-protein interaction (PPI) networks and gene-gene interaction networks were constructed. Functional roles were determined through Ontology (GO) term enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The XCell was employed to analyze the relationship between GPC2 expression and immune-related cells. Additionally, we retrospectively collected clinical data and survival information from a cohort of 51 patients diagnosed with NB and conducted immunohistochemistry (IHC) on the specimens as a validation set.
Results
Except for osteosarcoma, high expression of GPC2 was significantly associated with lower survival rates in the remaining six pediatric tumors, particularly NB and brain tumors. Notably, the MYCN amplified group exhibited significantly higher levels of GPC2 expression. Furthermore, GPC2 expression showed a positive correlation with infiltrating basophils, CD4 T cells, CD8 T cells, CD8 naïve T cells, Tgd cells, Th1 cells, Th2 cells and pro B cells, while demonstrating a negative correlation with infiltrating fibroblasts, macrophages M1 and M2 subtypes, monocytes neutrophils and pDCs. Among all 51 pediatric NB patients analyzed in this study, the MYCN amplified group displayed significantly higher levels of GPC2 expression compared to the MYCN not-amplified group. Additionally, survival analysis revealed that individuals with high GPC2 expression had significantly worse OS compared to those with low expression (P = 0.018).
Conclusion
A significant correlation was observed between elevated GPC2 expression and reduced survival rates in six pediatric tumors, particularly in cases of NB and brain tumors. Furthermore, the expression level of GPC2 in NB showed a positive association with MYCN status and levels of immune cell infiltration.
Interplay between antipredator behavior, parasitism, and gut microbiome in wild stickleback populations
Authors: Varg Javier Edo , Brealey Jaelle C. , Benhaïm David , Losada-Germain Rafael , Boughman Janette W.
Published: October 2024

Background
The role of predation stress in driving behavioral and microbial-host interaction changes is recognized, but the impact of microbial composition in aquatic organisms and its relationship with stress-related behavior remains poorly understood. This study explored the relationship between antipredator behavior, parasitism, and the gut microbiome in wild stickleback fish populations from two different lakes: Galtaból, clear and spring-fed versus Þristikla, turbid and glacial-fed. We aimed to identify potential links between these factors by analyzing behavioral responses to simulated predation, comparing microbiomes between populations with or without parasite infection, and examining potential correlations between behavior and microbiome composition.
Results
Behavioral analysis revealed differences between populations, with each exhibiting unique baseline behaviors i.e., higher activity in Galtaból fish and higher angular velocity in Þristikla fish, and varied responses to the presence of predator i.e., increased boldness in Galtaból fish and higher activity in Þristikla fish. The response to the predator attack was similar between fish from both populations. Parasitism influenced behavior, with parasitized fish displaying increased boldness. Microbiome analysis showed that a small proportion of its variation was explained by population, likely reflecting differences in lake environments. Only the marine genus Pseudoalteromonas abundance differed between populations. Parasitism in Galtaból fish population was linked to decreased alpha diversity in the microbiome, with an increase in specific microbial taxa, including potential pathogens, and a decrease in commensal microbes.
Conclusions
Our findings suggest that behavior and microbiome correlations may primarily reflect environmental adaptations and parasite status rather than direct gut-brain interactions. However, some tentative evidence suggests a potential innate connection between swimming activity, stress levels, and specific microbes. The study highlights the complexity of the gut-brain axis in wild populations and suggests future research directions, including experimental manipulations to uncover causal relationships between microbiome composition and behavior.
Pheromone release and perception, and fertility of insecticide-exposed cotton boll weevils (Anthonomus grandis grandis)
Authors: Omari Sharrine , Marra¹ D. Oliveira , Laumann Raul A. , Blassioli-Moraes Maria Carolina , Guedes Raul Narciso C. , Borges Miguel , Barbosa Wagner F. , Ferreira Marinaldo loures , Zanine Anderson M. , Batista Paulo S. C.
Published: October 2024

The objective of this work was to evaluate the effects of sublethal exposure to the insecticides malathion and beta-cyfluthrin on the pheromone perception and emission by the cotton boll weevil (Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae)). The potential impact of such sublethal exposure on this species population growth was also determined using fertility table parameters. Males and females were exposed to malathion at 10% of the label rate for 1 min, and to beta-cyfluthrin either at the full or 20% label rate also for 1 min. The response of insects exposed and non-exposed to pesticides to their aggregation pheromone (grandlure) was evaluated in Y-tube olfactometer and the pheromone production was evaluated. In order to evaluate the possible reproductive effects, the couples remained individualized for 48 hours and the females were subsequently evaluated until their death. The treatments were: 1) insecticide-exposed male and female; 2) exposed male; 3) exposed female; and 4) non-exposed males and females. Insects exposed to both insecticides did not show a response to their aggregation pheromone during the first 24 hours after the exposition. However, insecticide-exposed males did not have their pheromone production affected compared to non-exposed males. Sublethal insecticide exposure compromised the net reproductive rate (Ro) compromising the intrinsic rate of population growth (rm) of the cotton boll weevil.
Sleep Deprivation Increases Belief Change and Suppresses Confirmation Bias in Decision Tasks
Authors: Anckaert Charlotte , Peigneux Philippe , Gevers Wim
Published: October 2024

Belief updating, the process of revising one’s beliefs in light of new information, is subject to confirmation bias - the tendency to prioritize information that supports existing beliefs. How this process is affected by sleep deprivation (SD), which is known to impair cognitive function, remains underexplored. Here, we examined how SD alters belief change and confirmation bias in response to peer advice during a decision-making task. Across two studies, we found that SD substantially increased the likelihood of belief changes, with participants being more likely to change their beliefs following SD compared to after a full night of sleep. Even after SD, beliefs were still changed using logical reasoning, as participants continued to consider their initial confidence. At the same time, SD eliminated confirmation bias. Indeed, the typical confirmation bias asymmetry (i.e., a stronger increase in confidence when in agreement compared to a smaller drop in confidence when in disagreement) was no longer observed under SD. Our findings suggest that sleep loss undermines belief stability and dampens the cognitive biases that typically reinforce belief consistency. As a result, SD contexts may exert a significant impact on decision making processes, eventually leading to deleterious consequences in high stake situations.
THE ROLE OF ALTERED CAST IMPRESSION TECHNIQUE IN ACHIEVING ACCURATE PROSTHETIC FIT: A CASE REPORT
Authors:   Assistant Proffessor, Prosthodontics and Crown & Bridge, Vydehi Institute of Dental Sciences and Research Centre, Karnataka, India., S. Pavithra , S. Salagundi Basavaraj ,   Professor & HOD, Prosthodontics and Crown & Bridge, Coorg Institute of Dental Sciences, Virajpet, Karnataka, India., B.L Bhavana ,   Reader Prosthodontics and Crown & Bridge, Coorg Institute of Dental Sciences, Virajpet, Karnataka, India., N. Vishesh ,   Clinical Practitioner, MDS Prosthodontics and Crown & Bridge, Karnataka, India.
Published: October 2024

In dental practice, posterior bilateral edentulous spaces are most commonly encountered situations. Removable prosthesis generally is tooth or tissue borne or in combination. But the distal extension cases obtain their support from both the teeth and tissue adjacent to ensure equal distribution of forces on the teeth and the soft tissue. In this case report altered cast technique for fabrication of removable partial dentures has been described in detail. It is best alternative for impression making especially in distal extension cases for its better tissue adaptability, better support, uniform stress distribution, and stability of the prosthesis. Thus, increases patient comfort and function.
PITUITARY STALK INTERRUPTION SYNDROME REVEALED BY SEVERE AND PERSISTENT NEONATAL HYPOGLYCEMIA
Authors:   Faculty of Medicine and Pharmacy, Moulay Abdellah University, Fez, Morocco., Rahmouni S. , Skalli K. ,   Department of Neonatology and Neonatal Resuscitation Unit, University Hospital Hassan II, Fes, Morocco., Jerrar Oulidi I. ,   Department of Internal Medicine,MUSC Health., Ouanouche El. ,   Higher Institute of Nursing and Health Technology of Tanger- Morocco.,   Biology and Health Department. Faculty of Science. Ibn Tofail University Kenitra-Morocco., Stati S. ,   Psychiatric Emergency Department, Arrazi Psychiatric University Hospital, Sale-Morocco., Oukachou D. ,   Department of Neonatology and Neonatal Resuscitation Unit, University Hospital Hassan II, Fes, Morocco., Hmami F. ,   Faculty of Medicine and Pharmacy, Moulay Abdellah University, Fez, Morocco.,   Department of Neonatology and Neonatal Resuscitation Unit, University Hospital Hassan II, Fes, Morocco.
Published: October 2024

We present the case of a newborn who exhibited severe and persistent neonatal hypoglycemia without any apparent risk factors. The persistence of profound hypoglycemia, measured at 0.22 mmol/L, prompted an extensive biological workup that revealed a significant decrease of several pituitary hormones. The diagnosis of pituitary stalk interruption syndrome (PSIS) was confirmed by magnetic resonance imaging (MRI), which showed a complete interruption of the pituitary stalk. Prompt initiation of replacement therapy led to a notable clinical improvement and stabilization of blood glucose levels, thereby minimizing the risk of further hypoglycemic episodes.This case underscores the importance of broadening the etiological investigation in cases of persistent hypoglycemia without an obvious context, as hypoglycemia can be the sole clinical manifestation of PSIS. This congenital developmental anomaly of the pituitary gland represents a diagnostic emergency, and the prognosis is highly dependent on the prompt implementation of hormonal replacement therapy.
EFFECT OF DIFFERENT TYPES OF MUSIC ON CARDIO-RESPIRATORY VARIABLES AMONG OBESE YOUNG ADULTS: A LITERATURE REVIEW
Authors:   Dr. D.Y. Patil College of Physiotherapy, Pimpri Pune 411018., Burde Komal , Deshmukh Mayura ,   Dr. D.Y. Patil College of Physiotherapy, Pimpri Pune 411018., Palekar Tushar ,   Dr. D.Y. Patil College of Physiotherapy, Pimpri Pune 411018.
Published: October 2024

Background: Obesity is one of the major public health problems affecting every region of the globe. Obesity is a prevalent form of malnutrition that can lead to adverse metabolic effects. The risk of heart disease and diabetes increases steadily with increasing body mass index (BMI). Exercise physiology shows a growing interest in the possible physiological and psychological effects of music. Exercisers who listen to music are encouraged to keep up the effort while being diverted from their physical exhaustion. Objectives: To evaluate the effects of different types of music on cardio and respiratory Variables among overweight and obese young adults. Method: A review of observational, experimental, randomized control trials, and cross-sectional studies were performed. SCOPUS, Google Scholar, EBSCO and PubMed were searched using music, HRV, and obese, overweight young adults. Result: A total of 37 studies were identified of which 13 studies were published between 2011-2023 and were included in the study. 6 studies included in our study showed positive effects of music on the rate of perceived exertion, and cardiorespiratory fitness which could be a good strategy for obese young adults. Conclusion: This study concluded that there is a significant relationship between the different types of music along with exercise on cardiorespiratory fitness in young obese adults. The moderate quality of evidence in many studies indicates a need for further research to solidify these findings and explore new therapeutic avenues.
THE MORPHOLOGY OF GRIEVANCE
Authors: Singh Sodhi Jasjit
Published: October 2024

Employees/ Workers are the one of the important factors of production in any organisation. A grievance is a term that refers to any discord, discontent, or sense of injustice that arises between an employer and an employee in the workplace. There are various forms of grievances and methods of resolution. This topic will help the organisations to identify the Identify the grievance and its resolution. Worker voice is mainly concerned with Labour. All human beings are different in trainings, skills, Education, family background, attitude, & productivity. Every individual has set of expectations from the organisation and unmet expectations, stressful lives, and self-beliefs (including wicked thoughts) are some of the factors that lead to discontent.
PATHOEXTRACT: A BIOINFORMATIC PIPELINE FOR QUALITY CONTROL AND HOST DNA REMOVAL IN PLASMODIUM FALCIPARUM NGS DATA
Authors:   Laboratory of Mechanics and Computer Science (LAMI), Felix Houphouet-Boigny University, Abidjan, Cote dIvoire., Egomli Assohoun Stanislas ,   Parasitology and Mycology Unit, Pasteur Institute (IPCI), Abidjan, Cote dIvoire.,   Genomics and Metagenomics Platform, Pasteur Institute (IPCI), Abidjan, Cote dIvoire.,   Laboratory of Environmental Science and Technology (LSTE), Jean LorougnonGuede University (UJLoG), Daloa, Cote dIvoire., Berenger Ako Aristide ,   Parasitology and Mycology Unit, Pasteur Institute (IPCI), Abidjan, Cote dIvoire.,   Genomics and Metagenomics Platform, Pasteur Institute (IPCI), Abidjan, Cote dIvoire., Nguessan Akoguhi Patrice ,   Laboratory of Environmental Science and Technology (LSTE), Jean LorougnonGuede University (UJLoG), Daloa, Cote dIvoire., Christian Abouchou Ako Paul ,   Genomics and Metagenomics Platform, Pasteur Institute (IPCI), Abidjan, Cote dIvoire., Brou Kouassi Medard ,   Laboratory of Mechanics and Computer Science (LAMI), Felix Houphouet-Boigny University, Abidjan, Cote dIvoire., Adou Kablan Jerome ,   Laboratory of Mechanics and Computer Science (LAMI), Felix Houphouet-Boigny University, Abidjan, Cote dIvoire., Jambou Ronan ,   Parasitology and Mycology Unit, Pasteur Institute (IPCI), Abidjan, Cote dIvoire.
Published: October 2024

Malaria, caused by Plasmodium falciparum, is a significant global health burden, particularly in sub-Saharan Africa. Deep sequencing (NGS) of parasite genomes has revolutionized our understanding of its biology and the emergence of drug resistance. However, the presence of host human DNA and other microbial contaminants within patient samples can hinder accurate and efficient parasite genome analysis. To address this challenge, we have developed PathoExtract, a robust bioinformatics pipeline that integrates commonly used tools into a streamlined workflow. PathoExtract leverages Snakemake, a workflow management system, to provide a flexible and reproducible framework for data processing. The pipeline incorporates rigorous quality control steps to identify and remove low-quality reads and contaminants. Host DNA and microbial sequences are effectively filtered out using a combination of alignment-based and alignment-free methods, ensuring that only Plasmodium falciparum reads are retained for downstream analysis.The pipeline offers an intuitive graphical user interface, making it accessible to researchers with varying levels of bioinformatics expertise. This user-friendly interface simplifies the process of running the pipeline, even for those unfamiliar with command-line tools. The code and documentation for PathoExtract are freely available at: https://github.com/stanlasso/DREPAL-PATHOEXTRACT.
APPLYING THE ATTORNEY GENERALS PRINCIPLE OF OPPORTUNITY IN ECONOMIC CRIMES: A STUDY ON DIGNIFIED JUSTICE THEORY FOR NATIONAL WELFARE
Authors:   Faculty of Law, Universitas Pelita Harapan, Tangerang, Indonesia., Prasetyo Teguh
Published: October 2024

The concept of the oportunitas principle, originating from the now-obsolete Article 8 of Law No. 15 of 1961, has endured through subsequent legal frameworks in Indonesia. This principle has evolved to grant the Attorney General, the highest Public Prosecutor, exclusive authority for its implementation, aligning with the current Prosecution Law and the guidelines of the Criminal Procedure Code. The oportunitas principle allows the Attorney General to refrain from prosecuting certain criminal cases to safeguard the public interest, with a formal decision document issued to ensure legal certainty and emphasize the prohibition of future prosecution in cases dismissed in favor of the public, particularly regarding economic crimes that impact the national economy. Harmonizing this principle with the evolving legal landscape reflects a delicate balance between societal needs and legal frameworks, helping to prevent the misuse of power. The dynamic nature of the oportunitas principle underscores its vital role in the Indonesian legal system, providing a nuanced perspective on the intersection of justice, public interest, and prosecutorial discretion.
SUSTAINABLE FINANCE AND USE OF ARTIFICIAL INTELLIGENCE IN INVESTMENT DECISION MAKING
Authors: Bai R. Thanseena , R. Jeena , Shanavas Aziya
Published: October 2024

Artificial Intelligence (AI) is widely used across various industries to enhance efficiency, optimize decision-making, and create innovative solutions and it is now transforming the field of investment decision-making. As companies and investors place a greater emphasis on ethical and sustainable practices, sustainable finance-which incorporates environmental, social, and governance (ESG) factors into financial activities - is gaining global traction. Artificial Intelligence (AI) is becoming a vital instrument in the sustainable finance, because of its ability to analyse enormous volumes of data and spot patterns that are beyond human comprehension. This research paper examines the use of artificial intelligence (AI) tools like predictive analytics, machine learning, and natural language processing,in the financial decision-making. AI can quickly evaluate ESG aspects, predict long-term sustainability risks, and pinpoint high-impact investment opportunities that are in line with sustainability objectives by automating data gathering and analysis. Furthermore, AI-driven insights promote more ethical and knowledgeable financial decision-making by helping investors better manage risks related to social inequity, resource depletion, and climate change. Even though artificial intelligence (AI) has many benefits for sustainable finance, there are drawbacks as well, including data biases, opaque algorithms, and ethical issues. The article concludes that, artificial intelligence helps in creating more precise, effective, and socially conscious financial strategies.Artificial Intelligence (AI) is becoming increasingly important in sustainable finance decision-making due to its ability to handle complex, large-scale data, identify patterns, and optimize decision-making processes in ways that can advance sustainability goals. However, to fully realise AIs potential in forming a sustainable future for global finance, careful governance and ethical concerns are indispensable.
Analysis of tail dependence structure and risk spillover between cryptocurrencies
Authors: Abdulrahman Abubakar Abdulrazak , Clement Mba Jules , Ohonba Abieyuwa
Published: October 2024

Understanding the interconnectedness of cryptocurrencies based on their underlying technology is crucial for effective portfolio management and risk assessment. To establish the tail dependence structure and risk spillover between cryptocurrencies, this paper used the daily closing prices of the top eight proof-of-stake-based cryptocurrencies and the top ten proof-of-work-based cryptocurrencies from September 22, 2020 to April 7, 2023. This study applied the C-vine copulas and CoVaR measures. The outcome of the copula findings for the proof-of-stake cryptocurrencies illustrates that Ethereum exhibits strong resilience during market downturns, acting as a buffer for other proof-of-stake cryptocurrencies with pairwise tail dependence coefficients ranging from 0.45 to 0.67. Bitcoin Cash emerges as a portfolio diversifier within the proof-of-work ecosystem, absorbing 45% to 75% of volatility spillovers. However, from the proof-of-stake CoVaR analysis, ETH, DOT, and MATIC rank highest in systematic importance before April 2022, signifying their significant risk transmission role, and for the proof-of-work CoVaR analysis, Bitcoin (BTC) is the primary risk transmitter in the cryptocurrency portfolio, having a positive CoVaR of 0.15. Ethereum and Bitcoin are identified as the dominant risk transmitters within their respective groups, highlighting their potential to amplify systemic risk. This study provides valuable insights for investors and policymakers navigating the increasingly complex cryptocurrency landscape.
Sorbic acid in cheese making
Authors: Orlova Elena , Mordvinova Valentina , Onosovskaya Natal'ya
Published: October 2024

The domestic food industry is currently focusing on food products with long shelf life. Extended storage is usually a result of various aseptic packaging techniques. However, preservatives also prolong shelf-life. Such food additives can be applied only after a comprehensive and multifaceted study of their safety and possible impact on consumer health. New studies on negative effects of certain additives make it necessary to review their safety status. Sorbic acid and its salt E202 are common preservatives to be found in sauces, canned food, chocolate, jam, sausages, fruit juices, fish, bakery, confectionery, margarine, oil, butter, wine, carbonated drinks, etc. In Russian cheese production, sorbic acid and potassium sorbate serve as a surface treatment of long-maturing cheeses: they protect cheese wheels from mold, thus reducing product losses. These preservatives can be part of protective coatings, packaging matrix, and smart packaging, especially in flavored cheeses and particular wrapping types. Food preservatives require a strict qualitative and quantitative control to prevent adverse effects on consumer health, as well as elaborate labelling rules.
Effect of Cathodic Electrochemical Activation of Milk on Beneficial Properties of Butter
Authors: Pochapskaya Viktoria , Soboleva Nataliya , Lyashenko Valentina
Published: October 2024

Pasteurization and sterilization of milk are known to affect its biological properties. Cathodic electrochemical activation offers a prospective alternative to the abovementioned processes. It involves soluble amino acids of whey proteins that are selected from α-lactoalbumins and β-lactoglobulins, including such amino acids as asparagine, glycine, glutamine, serine, threonine, and tyrosine at 0.5 wt. %. Cathodic electrochemical activation provides a long-term preservation of antioxidant properties: titratable acidity ≤ 17 °T, active acidity (pH) 8-10.5; pathogenic microflora ≤ 8×104 (CFU)/cm3. In this study, the butter sample obtained from electrochemically activated milk demonstrated better sensory properties than the control. As for the physicochemical variables, the experimental sample had a 2.5% higher moisture level while the mass fraction of fat was 81.5% and the titratable acidity was slightly lower than that of the control sample (by 0.3%). After three months of storage, the increase in yeast and mold was by 3 CFU/g in the control sample and by 2 CFU/g in the experimental one. The method of cathodic electrochemical activation proved suitable for industrial butter production.
Image of Social World in Metaphors and Narratives of Adults
Authors: Alperovich Valeriya D.
Published: October 2024

The image of the social world is a predictor of social behavior and its development during stable social transitivity, i.e., COVID-19 pandemic, economic crises, local military conflicts, etc. It can also be interpreted as a variable linguistic phenomenon. However, very little empirical research has featured its integral structural and content characteristics through the prism of narratives, metaphors, and social representations. The author used the methods of thematic narrative analysis and content analysis of metaphors to identify the narrative constructions used by adult subjects to describe their image of the social world. The data obtained were subjected to frequency analysis and hierarchical cluster analysis. The experiment involved 193 respondents aged 35–49 years old, employed in the city of Rostov-on-Don, Russia. In their metaphors and narratives, they described the social world as balancing between stability and dynamism, predictability and stochasticity, as well as endowed it with differentiation in the absence of universal laws. The rationality of the metaphors reflected the age identity while the subject of biographical narratives reflected the subjective adaptation to current social processes.
Cost-effective non-revenue water reduction: analysis through pilot activities in Kigali City, Rwanda
Authors: Ogata Ryuji , Tsutsui Nobuyuki , Bahige Jean Berchmans , Murakami Shin
Published: October 2024

Non-revenue water (NRW) is a major problem for water utilities, and its reduction has become an important challenge in ensuring a sustainable water supply. However, a lack of clarity regarding the cost–benefit analysis of investment in pilot activities to reduce NRW may hinder further investment in such projects. Thus, we attempted to identify strategies for effective NRW reduction and establish a method for estimating their cost-effectiveness. To this end, a project to strengthen the NRW management capacity and build a sustainable NRW reduction system was implemented in Kigali City, Rwanda, between 2016 and 2021. The project included four objectives: enhancement of the planning capacity for NRW reduction; acquisition of basic knowledge, skills, and techniques for NRW control; enhancement of staff capacity to execute NRW reduction measures; and establishment of a system to accurately measure NRW ratios. The project helped to formulate a 5-year plan for NRW reduction and 596 employees of Kigali City were trained for the same. The NRW ratios were reduced by 10 and 12% in the two pilot areas of the city. Cost–benefit analysis revealed the effectiveness of the investment in NRW reduction activities over time.
A new virtual water qualimeter with a changeable broken linear pointer based on a quadrant circular constellation graph
Authors: Yang Won-Chol , Ri Jae-Bok , Om Myong-Song , Kim Un-Ha , Ri Wi-Song , Sok Sun-Hak
Published: October 2024

As water is a very important natural resource for humans and its quality is closely related to human health, water quality evaluation (WQE) is very important for the protection of human health, livelihoods, environment, and management of water. Although various WQE methods have been developed and used, a common drawback is that the methods lack visualization of the WQE results and require additional complicated graphical techniques. The Pointer meter is an analogue measuring device with a pointer indicating the measured value with its location. A constellation graph (CG) enables to map multi-dimensional data on a two-dimensional plane. By introducing the pointer meter and CG for WQE, this paper proposes a new visualized pointer meter-type virtual water qualimeter (VWQ-meter) based on a quadrant circular constellation graph (QCCG). It has a changeable broken linear pointer with variable lengths and shapes, while the traditional pointer meters have straight-line pointers with a fixed length and a straight-line shape. It evaluates the water quality not only quantitatively but also intuitively. To demonstrate its effectiveness, it is applied to one example of WQE. It could be widely applied to not only WQE but also various multi-criteria decision-making (MCDM) problems arising in water and wastewater treatment.
Riverine CH4 and CO2 emissions respond to associated agricultural land use and seasonality
Authors: Tang Jianfei , Chen Hao , Ye Jianfeng , Zhu Yi , Zhang Jinxu , Chen Yu , Ma Changwen
Published: October 2024

Recently, agricultural land use has significantly impacted carbon emissions and water quality in adjacent rivers. This study examined the influence of land-use types and seasonality on carbon emissions from agriculture-impacted rivers in Shanghai. Our results showed that the crop farming land-use-associated river (AR) had CH4 and CO2 fluxes 2.68 and 3.47 times higher, respectively, than those in the aquaculture land-use-AR and 2.86 and 2.81 times higher than in the livestock and poultry breeding land-use-AR. Statistical analyses revealed that total nitrogen, ammonia nitrogen, and humification index were significantly correlated with crop farming land-use-AR carbon emissions. Conversely, no clear correlation was found between chemical factors and carbon fluxes in the aquaculture land-use-AR and livestock and poultry breeding land-use-AR due to the complexity of pollution sources. Seasonality significantly influenced CH4 and CO2 fluxes from agriculture-impacted rivers, with carbon fluxes in summer being 3.5 and 2.5 times higher than in winter, respectively. Crop farming land-use-AR carbon fluxes exhibited the highest variability under seasonal influences. This study emphasizes the need for policies to mitigate agricultural land use impacts on river carbon emissions.
Wuzi-Yanzong-Wan Prevents the Defect of Cell-Cell Junctions between Sertoli-Germ Cells by Up-Regulating the Expression of TAp73
Authors: Li Li , Pan Min , Liu Ziao , Zhao Hongsu , Xu Fengqing , Tong Xiaohui , Wang Yajuan , Chen Bin , Yu Lihua , Wang Tongsheng
Published: October 2024

Background:

The TAp73 gene is an anti-cancer gene that also affects the junction between
Sertoli and germ cells. Inhibition of this gene causes infertility in male mice. Our previous
research proved that Wuzi-Yanzong-Wan (WZYZW) can protect spermatogenesis and maturation
by preventing TAp73 inhibition.

Objective:

This study aimed to investigate the effect of drug-containing serum of WZYZW on
the defect of cell-cell junctions in the Sertoli-germ cells co-culture system in vitro.

Methods:

LC-HRMS was used to analyze the content of active ingredients in WZYZWmedicated
serum. Then, primary extraction and co-culture of germ cells and Sertoli cells were
carried out. Co-cultured cells were added with PFT-α to induce the TAp73 inhibition model,
with WZYZW-medicated serum at 2.5%, 5%, and 10% treated in parallel. Sloughing of germ
cells from Sertoli cells was calculated. Transmission electron microscopy (TEM), Immunofluorescence,
qRT-PCR, and western blot methods were employed.

Results:

The drug-containing serum of WZYZW contained schisandrin, hyperoside, geniposidic
acid, ellagic acid, and quercetin. Using TEM assay, we observed restoration of the desmosomelike
(Des), tight junctions (TJ), and basal ectoplasmic specialization (ES) structure following
WZYZW treatment. WZYZW caused inhibition of peptidase and protease inhibitors (tissue inhibitor
of metalloproteinase-1 (TIMP1), Serpina3n) by immunofluorescence analysis. Western
blot and qRT-PCR analysis revealed that WZYZW was able to ameliorate the expressions of
peptidase and protease inhibitors and cell adhesion factors, such as TAp73, TIMP1, Serpina3n,
Desmocollin-3, N-cadherin, and Nectin-2.

Conclusion:

WZYZW-medicated serum could prevent the defect of cell-cell junctions between
Sertoli-germ cells co-culture system in vitro by up-regulating the expression of TAp73.