bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Ultrasonic-Assisted Preparation of Water Caltrop Starch–Lipid Complex: Structural and Physicochemical Properties
Authors: Huang Kuan-Wei , Lai Lih-Shiuh
Published: January 2025

This study investigates the effect of ultrasonic-assisted preparation on the structural and physicochemical properties of water caltrop starch-palmitic acid complexes as a function of ultrasound intensity and treatment time. All samples exhibited the characteristic birefringence of starch-lipid complexes under the polarized microscope, and flake-like and irregular structure under scanning electron microscope (SEM), indicating the formation of complexes through ultrasonic-assisted preparation. X-ray diffraction pattern further confirmed the transition from the original A-type structure for native starch to V-type structure for starch-lipid complexes, and the relative crystallinity of starch-lipid complexes increased as the ultrasound intensity and treatment time increased. Attenuated total reflectance-Fourier-transform infrared spectroscopy (ATR-FTIR) analysis indicated a decreasing trend in absorbance ratio at wavenumber of 1022 cm−1/995 cm−1, suggesting that the increase in the complex promoted the self-assembly within the short-range ordered structure, leading to the formation of bonds between the complexes. However, rapid-visco analysis (RVA) demonstrated that the viscosity generally decreased as the ultrasound intensity and treatment time increased, possibly due to the reduction in molecular weight by ultrasound. Differential scanning calorimetric (DSC) analysis revealed that the control starch-lipid complex without ultrasound treatment (US-0-0) exhibited two distinct endothermic peaks above 90 °C, representing Type I (95–105 °C) and Type II (110–120 °C) V-type complexes. However, ultrasound-treated samples showed only one peak around 95–105 °C and increased enthalpy (∆H), which was likely due to the breakdown of amylose and amylopectin, leading to more complex formation with palmitic acid, while the resulting shorter chains in the ultrasound-modified sample favor the formation of Type I complexes.
Effect of Carbohydrate Formulas on Instrumental and Sensory Parameters in Dry-Fermented Iberian Pork Sausages
Authors: Sánchez-Giraldo Maite , Vioque-Amor Montserrat , Gómez-Díaz Rafael , Clemente-López Ignacio , Amaro-López Manuel Ángel , Avilés-Ramírez Carmen
Published: January 2025

Dry-fermented sausages are appreciated all over the world for their sensory characteristics. Carbohydrates (sugars) are added during the production process, the type and quantity of which directly affect the quality of this product. However, there are few data on the role of sugars on instrumental and sensory parameters of sausages derived from Iberian pork. The objective was to determine the effect of different carbohydrate formulas during the ripening time on the quality of dry-fermented Iberian pork sausages. Five batches were formulated with different combinations and concentrations of carbohydrates (dextrose, dextrin and glucose syrup), making 16 sausages per batch on which to perform repeated measurements throughout the ripening process. Physicochemical characteristics, instrumental colour, textural parameters and sensory attributes were analysed. The C− batch (without any carbohydrate addition) showed unstable pH evolution and high b* values throughout the ripening process and the lowest instrumental texture values (for hardness, springiness and chewiness). The D10d5 batch also presented an unstable pH evolution but low a* values, and it was the highest rated by consumers for sensory texture attributes. This batch, made with dextrose (in higher proportion) and dextrin, differed from the other Iberian pork sausages, suggesting that this carbohydrate formula may be more appreciated by Mediterranean consumers.
Antimicrobial Effectiveness of Clove Oil in Decontamination of Ready-to-Eat Spinach (Spinacia oleracea L.)
Authors: Armah Abigail A. , Ofori Kelvin F. , Sutherland Kenisha , Otchere Emmanuel , Lewis Winter A. , Long Wilbert
Published: January 2025

Due to an increased demand for natural food additives, clove oil was assessed as a natural alternative to chemical disinfectants in produce washing. This study assessed the antimicrobial activity of 5 and 10% (v/v) clove oil-amended wash liquid (CO) using a zone of inhibition (ZIB) test and determined the time required to completely inactivate pathogenic bacteria using bacterial death curve analysis. A washing experiment was used to evaluate CO’s ability to inhibit bacterial growth on inoculated RTE spinach and in the wash water. The findings showed that Shigella flexneri, Salmonella Typhimurium, and Salmonella enterica recovery were completely inhibited within 5 min. Escherichia coli and Staphylococcus aureus recovery were completely inhibited at 10 and 30 min, respectively. The ZIB test showed that 5% CO had the highest inhibitory effect on both Salmonella strains and E. coli with approximately 10 mm ZIB diameter. Additionally, 5% CO completely inactivated all bacterial strains on spinach samples and in the wash water except for S. aureus. A total of 80 mg/L peracetic acid (PAA) resulted in >2log CFU/mL recovery on experimental washed samples. These findings suggest that 5% CO was highly effective in inhibiting microbial growth on RTE spinach, potentially contributing to sustainable food safety and shelf-life extension strategies.
The Role of Fashion and Art in First Nations Healing, Decolonisation, and Cultural Practice
Authors: Clark Treena
Published: January 2025

This paper explores the intersection of art, fashion, and decolonisation within First Nations communities, focusing on how clothing and adornment are powerful tools for healing, cultural survival, and resistance. Through art programs, fashion workshops, and community-based projects, First Nations peoples reconnect with culture, land, and history, fostering identity and continuity while addressing the traumas of colonisation. This paper examines how First Nations artists and designers engage with traditional garments, such as kangaroo skin cloaks and shell necklaces, to reclaim ancestral practices and challenge colonial traumas. By reinterpreting colonial clothes and creating new forms of fashion, artists engage in truth-telling, amplify resilience, and promote reconciliation. This paper highlights the role of art and fashion as an aesthetic expression and a strategy for cultural survival and resistance. It concludes by offering recommendations for policies and programs that support First Nations fashion initiatives, fostering economic opportunities, social well-being, and intergenerational healing. Ultimately, this paper advocates for the transformative power of art and fashion as pathways to decolonisation and empowerment for First Nations communities.
Identifying Genetic Predisposition to Dozer Lamb Syndrome: A Semi-Lethal Muscle Weakness Disease in Sheep
Authors: Stegemiller Morgan R. , Highland Margaret A. , Ewert Kathleen M. , Neaton Holly , Biller David S. , Murdoch Brenda M.
Published: January 2025

Background: Lamb health is crucial for producers; however, the percentage of lambs that die before weaning is still 15–20%. One factor that can contribute to lamb deaths is congenital diseases. A novel semi-lethal disease has been identified in newborn Polypay lambs and termed dozer lamb syndrome. This study aims to determine if there is a genetic predisposition to dozer lamb syndrome. These lambs are weak and unable to lift their heads, suckle, and swallow, resulting in nasal reflux. Methods: Genetic analyses, including a genome-wide association, runs of homozygosity, and fine mapping to determine haploblock within regions of interest, were utilized in determining genetic predispositions to dozer lamb syndrome. Results: The genome-wide association study identified a region of chromosome 15 with three significant SNPs (p-values of 6.81 × 10−6, 5.71 × 10−6, and 8.52 × 10−6). Genetic analysis identified a run of homozygosity on the same region of chromosome 15 with an odds ratio of 236.7. Fine mapping of this region identified three haploblocks associated with the dozer lamb syndrome (p-value = 2.41 × 10−5). Conclusions: The most significant and promising gene in this region is CELF1, which is known to play an important role in muscle development. Abnormal CELF1 abundance and cellular location are reported to result in abnormal muscle development. Identification of genetic aberrations associated with dozer lamb syndrome provides a tool for decreasing or eliminating the genotype and, thus, the associated phenotype(s) from Polypay sheep.
Beyond Anything Realism Can Represent? Monstrous Crime in Marx’s Victorian Novel
Authors: Althofer Jayson
Published: January 2025

This article reads Karl Marx’s Capital (volume 1, 1867) as the Bildungsroman of a congenital criminal: its eponymous character, Capital. Following Friedrich Engels’s The Condition of the Working Class in England (1845), Marx detects and dissects capitalism’s crimes. Capital has been called Marx’s ‘Victorian novel’ and compared to English realism’s triple-deckers. Yet his indispensable informants include factory inspectors whose reports, according to Fredric Jameson, provide testimony beyond anything realism can represent. How, then, does Marx’s apparently realist aesthetic convey Capital’s criminal deeds and criminogenic drive? To address this matter, the article examines the Gothicism of Marx’s realism. It highlights his development of Engels’s Gothic realism, demonstrates how Capital begins in media res—its first sentence presenting an immense, monstrous collection of evidence of Capital’s cannibalism—and links this opening crime scene to Marx’s portrayal of the 1863 case of Mary Anne Walkley. Murdered in her workplace, Walkley inhabits an underworld overpopulated by fellow workers killed by wage-labour. The article argues that, because actuality under the rule of Capital is structurally and monstrously criminal, Marx’s Gothic constitutes a realistic medium to represent criminal monsters and structures.
Exploring Musical Feedback for Gait Retraining: A Novel Approach to Orthopedic Rehabilitation
Authors: Cedin Luisa , Knowlton Christopher , Wimmer Markus A.
Published: January 2025

Background/Objectives: Gait retraining is widely used in orthopedic rehabilitation to address abnormal movement patterns. However, retaining walking modifications can be challenging without guidance from physical therapists. Real-time auditory biofeedback can help patients learn and maintain gait alterations. This study piloted the feasibility of the musification of feedback to medialize the center of pressure (COP). Methods: To provide musical feedback, COP and plantar pressure were captured in real time at 100 Hz from a wireless 16-sensor pressure insole. Twenty healthy subjects (29 ± 5 years old, 75.9 ± 10.5 Kg, 1.73 ± 0.07 m) were recruited to walk using this system and were further analyzed via marker-based motion capture. A lowpass filter muffled a pre-selected music playlist when the real-time center of pressure exceeded a predetermined lateral threshold. The only instruction participants received was to adjust their walking to avoid the muffling of the music. Results: All participants significantly medialized their COP (−9.38% ± 4.37, range −2.3% to −19%), guided solely by musical feedback. Participants were still able to reproduce this new walking pattern when the musical feedback was removed. Importantly, no significant changes in cadence or walking speed were observed. The results from a survey showed that subjects enjoyed using the system and suggested that they would adopt such a system for rehabilitation. Conclusions: This study highlights the potential of musical feedback for orthopedic rehabilitation. In the future, a portable system will allow patients to train at home, while clinicians could track their progress remotely through cloud-enabled telemetric health data monitoring.
Swimmer’s Shoulder: Ultrasound Anatomical Description of Shoulder Tendons in Elite Swimmers and Water Polo Players
Authors: Arrillaga Beatriz , Pérez-Bellmunt Albert , García Iker , Rodríguez-Adalia Luis , Möller Ingrid , Miguel-Pérez Maribel
Published: January 2025

Objectives: This study aimed to examine the differences in shoulder ultrasonographic parameters between overhead aquatic athletes and their relationship with shoulder pain. Methods: Sixty-four healthy junior subjects (n = 128 shoulders) participated in the study, including 17 healthy controls, 25 elite junior swimmers, and 22 elite junior water polo players. An ultrasonographic study of the shoulder was carried out to measure the thickness of the rotator cuff structures in sex- and age-paired groups. Results: Compared to controls, female and male swimmers and water polo players had thicker values in the rotator cuff tendons, including the subscapularis (SSB) (p < 0.001), supraspinatus (p < 0.001), infraspinatus (p < 0.001) and teres minor (Tm) (p < 0.025). The female swimmers had thicker values than the water polo players in the SSB (p < 0.001) and Tm (p < 0.011). In the comparison by sexes, the female controls had a thinner LHBB, SSB and Tm (p < 0.019), the female swimmers had a thinner ISP (p = 0.001), and the female water polo players had a thinner LHBB, SSB, ISP and acromiohumeral distance than their group-paired counterparts (p < 0.018). Finally, the females had a lower incidence of positive Jobe test results compared to the males (p = 0.018), which was associated with lower pain scores at rest (p < 0.034) and during training (p < 0.036). Conclusions: The rotator cuff tendon structures were larger in the healthy overhead aquatic athletes compared to the age- and sex-paired controls. The females had smaller values in the shoulder ultrasonographic tendon parameters compared to the age- and sport-paired males, except for the supraspinatus tendon.
Occurrence and Behavior Analysis of Duponchelia fovealis on Strawberry Plants: Insights for Integrated Pest Management
Authors: Gonçalves Rodrimar Barboza , Zawadneak Maria Aparecida Cassilha , Kuhn Taciana Melissa de Azevedo , Gulinelli Thales Fernando Moreno , Pimentel Ida Chapaval , Poltronieri Alex Sandro , da Rosa Joatan Machado , Mirás-Avalos José Manuel , Araujo Emily Silva
Published: January 2025

The European pepper moth, Duponchelia fovealis (Lepidoptera: Crambidae), is a key pest to strawberries in America and Europe. Understanding its behavior in the field can support integrated management strategies. In this work, field surveys were conducted to confirm the presence of this pest in commercial areas within the State of Paraná (Brazil) and to determine on which plant organ it prevailed. Semi-field experiments evaluated oviposition preference as a function of strawberry cultivar. Based on pest behavior, insecticide distribution was assessed following conventional field applications. Our results determined that D. fovealis spread within a 400 km radius from the site in which it was first recorded in Paraná, and the infested area reached 68.2% by the end of the survey. This species concentrated on basal leaves and crowns, where more than 90% of the larvae were collected. Moreover, the number of eggs per plant was significantly higher in the ‘Albion’ cultivar. The sprayed insecticide remained in the upper and middle thirds of the strawberry plant canopy, not reaching the organs where D. fovealis larvae were mainly detected. This study provides useful information on the cryptic habit of this pest that may help in designing efficient monitoring and control strategies.
Fruit Quality and Production Parameters of Some Bitter Cherry Cultivars
Authors: Perju Ionel , Mineață Iulia , Sîrbu Sorina , Golache Iuliana Elena , Ungureanu Ionuț Vasile , Jităreanu Carmen Doina
Published: January 2025

Bitter cherries (Prunus avium var. sylvestris Ser.) represent a valuable raw material in the traditional Eastern European food industry with high potential within the horticultural chain and circular economy in the context of global food security due to exceptional nutritional properties. The present study was carried out in the period 2022–2024 and had as its main purpose the evaluation of the fruit quality and production indices of some bitter cherry cultivars suitable for the technological norms specific to industrial processing. Five bitter cherry cultivars (C1-Amaris, C2-Amar Maxut, C3-Amar Galata, C4-Silva, C5-Amara) were studied and analyzed in terms of fruit quality—morpho-physiological and organoleptic traits, and physical and chemical parameters—and general productivity—tree vigor, fruiting, and yield indices. The results highlighted a wide variability in the physical characteristics of bitter cherries, with an average weight between 3.3 and 4.9 g and the color of the skin varying from yellow with redness to dark red and blackish. Regarding the chemical attributes, antioxidant activity was relatively higher in fruits with a more intense bitter taste (89.3 μg Trolox·g−1 f.w for C2 and 89.1 μg Trolox·g−1 f.w. for C4 and C5), a fact also found in the content total of polyphenols (with a maximum value of 743.2 mg GAE·100 g−1 f.w at C2). Total soluble solids content had an average value of 20.51°Brix and titratable acidity of 0.85 g malic acid·100 g−1 f.w. The influence of local environmental factors on the productivity of bitter cherry cultivars was highlighted by significant statistical differences (p < 0.05) between cultivars. Thus, the resistance to frost in the full flowering phenophase had an average value of 86.69%, and regarding the resistance to fruit cracking, the highest percentage was found in C1, with 99.79% unaffected fruits. The productivity index per tree had an average value of 0.24 kg per cm2 trunk cross-section area. The physico-chemical properties of the fruits and the productivity of bitter cherry cultivars support the possibility of their efficient use in processing and the food industry, yielding high-quality products with nutraceutical value.
Assessing Groundwater Connection/Disconnection to Waterholes Along the Balonne River and in the Barwon–Darling River System in Queensland and New South Wales, Australia, for Waterhole Persistence
Authors: Hofmann Harald , Marshall Jonathan
Published: January 2025

Waterholes in semi-arid environment are sections of rivers that fill during high river flows or floods and keep water once flow ceases. They are essential water sources for rive ecosystems. Some waterholes remain even during prolonged droughts. The resilience of ecosystems in these environments depends on the persistence of the waterholes. While most semi-arid, ephemeral river systems are disconnected from regional groundwater and losing in most parts there may be some sections that can be connected to localised groundwater or parafluvial areas. To assess the persistence of waterholes the groundwater contribution to the water balance needs to be addressed. This study assesses groundwater connectivity to waterholes in a part of the Murray-Darling Basin, one of the largest watersheds in the world, using environmental tracers radon and stable isotopes. Approximately 100 samples were collected from 27 waterholes along the Narran, Calgoa, Barwon and Darling rivers, as well as 8 groundwater bore samples. The assessment of groundwater connectivity or the lack of is necessary from water balance modelling and estimation of persistence of these waterholes. As expected, the results indicate consistently low radon concentrations in the waterholes and very small deviation in stable isotopes δ18O and δ2H. In general, most of these waterholes are losing water to groundwater, indicated by low salinity (EC values) and low radon concentrations. While radon concentrations are small in most cases and indicative of little groundwater contributions, some variability can be assigned to bank return and parafluvial flow. It indicates that these contributions may have implications for waterhole persistence in ephemeral streams. The study demonstrates that in some cases local bank return flow or parafluvial flow may contribute to waterhole persistence.
Characterization of the Prevalence and Antibiotic Resistance of Staphylococcus Species in an Exercise Facility in Central Kentucky, USA
Authors: Kiborus Lilian Jeptoo , Altheide S. Travis , Marion Jason W.
Published: January 2025

The spread of methicillin-resistant Staphylococcus aureus (MRSA) in community settings, including fitness/exercise centers, remains relevant for public health. MRSA, a cause of severe infections in some, can be transmitted through shared equipment and skin contact. Understanding its prevalence and the frequency of antibiotic resistance in such environments can be useful for informing hygiene and intervention strategies. For investigating, multiple environmental swabs were collected from 14 different sites within a fitness facility, including equipment and locker rooms. Samples were collected for characterizing the prevalence of staphylococci (including MRSA), E. coli, and carbapenem-resistant Enterobacterales (CRE). Isolated colonies were identified biochemically and evaluated for antibiotic resistance. Logistic regression was applied to assess risk across different surfaces. Among 42 samples, the highest prevalence of Staphylococcus spp. was on locker room surfaces. S. aureus was prevalent on locker room floors and benches. Non-S. aureus species, such as S. saprophyticus and S. haemolyticus, were common. Resistance to oxacillin and penicillin was widespread, particularly among non-S. aureus species. E. coli was detected once, and CRE was not detected. Fitness center surfaces can harbor staphylococci, including MRSA. The results obtained corroborate other studies finding notable antibiotic resistance among staphylococci from fitness center surfaces. Hygiene improvements, including personal hygiene actions, are essential for reducing transmission risks.
Differential Life Satisfaction in a German Representative Sample
Authors: Arnold Christopher , Muschalla Beate
Published: January 2025

Life satisfaction includes various aspects, such as satisfaction with work, family, environment, and finances, and is influenced by sociodemographic and socioeconomic factors. This representative study investigates differential life satisfaction in the general population and its associations with sociodemographics. The study used a cross-sectional design with 2522 German participants, collected via face-to-face interviews and three-stage random sampling, assessing satisfaction across 17 life areas with the Differential Life Burden Scale. Overall life satisfaction was high (M = 4.46, scale from 1 to 6). Although globally satisfied, most participants (84.2%) named at least one negative area of life. Politics and environment were perceived as dissatisfying (M = 3.1; M = 3.81, respectively); social contacts and leisure time were evaluated as rather satisfying (M about 5.00). Age, income, and unemployment were associated with life satisfaction. Gender and age were differently associated with life domains: Older people were less satisfied with their health. Younger people were more satisfied with leisure time. Younger were less satisfied with their children than older participants. The German population is generally satisfied with life, though factors like age, unemployment, and income influence the number of negatively perceived life domains. This highlights the importance of evaluating specific life areas in addition to overall life satisfaction for a more comprehensive understanding.
Spatial Analysis of Maritime Disasters in the Philippines: Distribution Patterns and Identification of High-Risk Areas
Authors: Aguilar Glenn D. , Tirol Yasmin P. , Basina Ryan M. , Alcedo Jamaica
Published: January 2025

Maritime accidents frequently occur in the Philippine archipelagic waters, often resulting in significant loss of life. These incidents highlight the urgent need for improvements in the country’s maritime safety systems. By utilising accident data from the Philippine Coast Guard and the GISIS IMO databases, spatial analytical approaches were employed to determine incident distribution patterns and resulted in an overall depiction of the likelihood component of risk across the country’s territorial waters. Kernel density and hotspot analysis revealed areas where incidents were concentrated and where statistically significant hotspots occurred. The Maxent tool was used to develop risk likelihood models for the incident locations using environmental rasters representing wind speed, significant wave height, depth, surface current, land distance and port distance. Model performance metrics including the AUC, TSS and Kappa were used to compare the two datasets and provide confidence on model robustness. Variable contribution figures showed that land distance is the most influential variable, with the majority of high-risk areas predominantly located near population centres. The resulting maps provide an intuitive and informative depiction of the characteristic patterns of maritime accidents in the country, identify areas of high risk requiring immediate attention and offer valuable insights to support strategies for improving and enhancing the country’s maritime safety.
Characterizing Circulating microRNA Signatures of Type 2 Diabetes Subtypes
Authors: Sulaiman Fatima , Khyriem Costerwell , Dsouza Stafny , Abdul Fatima , Alkhnbashi Omer , Faraji Hanan , Farooqi Muhammad , Al Awadi Fatheya , Hassanein Mohammed , Ahmed Fayha , Alsharhan Mouza , Tawfik Abdel Rahman , Khamis Amar Hassan , Bayoumi Riad
Published: January 2025

Type 2 diabetes (T2D) is a heterogeneous disease influenced by both genetic and environmental factors. Recent studies suggest that T2D subtypes may exhibit distinct gene expression profiles. In this study, we aimed to identify T2D cluster-specific miRNA expression signatures for the previously reported five clinical subtypes that characterize the underlying pathophysiology of long-standing T2D: severe insulin-resistant diabetes (SIRD), severe insulin-deficient diabetes (SIDD), mild age-related diabetes (MARD), mild obesity-related diabetes (MOD), and mild early-onset diabetes (MEOD). We analyzed the circulating microRNAs (miRNAs) in 45 subjects representing the five T2D clusters and 7 non-T2D healthy controls by single-end small RNA sequencing. Bioinformatic analyses identified a total of 430 known circulating miRNAs and 13 previously unreported novel miRNAs. Of these, 71 were upregulated and 37 were downregulated in either controls or individual clusters. Each T2D subtype was associated with a specific dysregulated miRNA profile, distinct from that of healthy controls. Specifically, 3 upregulated miRNAs were unique to SIRD, 1 to MARD, 9 to MOD, and 18 to MEOD. Among the downregulated miRNAs, 11 were specific to SIRD, 9 to SIDD, 2 to MARD, and 1 to MEOD. Our study confirms the heterogeneity of T2D, represented by distinguishable subtypes both clinically and epigenetically and highlights the potential of miRNAs as markers for distinguishing the pathophysiology of T2D subtypes.
MicroRNAs in Plasma-Derived Extracellular Vesicles as Non-Invasive Biomarkers for Eosinophilic Esophagitis
Authors: Grueso-Navarro Elena , Rodríguez-Alcolado Leticia , Arias-González Laura , Aransay Ana M. , Lozano Juan-José , Sidorova Julia , Juárez-Tosina Rocío , González-Cervera Jesús , Lucendo Alfredo J. , Laserna-Mendieta Emilio J.
Published: January 2025

Eosinophilic esophagitis (EoE) is a chronic inflammatory esophageal disorder. The lack of non-invasive biomarkers currently results in dependency on endoscopy with biopsies for its diagnosis and monitoring. We aimed to identify potential non-invasive biomarkers using microRNAs (miRNAs) in plasma-derived extracellular vesicles (pEVs). This was a prospective single-center observational study of a discovery cohort of EoE patients (n = 26) with active disease (EoE.Basal) and after anti-inflammatory treatment (EoE.Post.tx) and control subjects (n = 16). Small-RNA-seq was performed to identify differentially regulated small RNAs (sRNAs). Candidate miRNAs were validated in an independent cohort (EoE patients, n = 33; controls, n = 14). The pEVs-sRNA cargo differed among conditions. Compared with controls, Ser_Comb_22, Leu_Comb_5, miR-10b-5p, and miR-125a-5p were upregulated in EoE.Basal, and miR-224-5p, miR-221-3p, let-7d-5p, and miR-191-5p were downregulated. The combination of miR-221-3p and miR-10b-5p showed the best diagnostic performance. Comparing paired EoE samples, miR-374a-5p and miR-30a-3p were upregulated in EoE.Basal, while miR-15a-5p and let-7d-5p were downregulated. Combined miR-30a-3p and miR-15a-5p showed the best AUC values, and miR-30a-3p alone was best as a monitoring biomarker (p = 0.001). In conclusion, pEVs-sRNA changed upon inflammation in EoE patients, and miR-30a-3p was proposed as a potential biomarker for monitoring the treatment. This study was the first to explore the use of pEVs as a non-invasive biomarker for EoE.
Crystal Structures of Antigen-Binding Fragment of Anti-Osteocalcin Antibody KTM219
Authors: Yazaki Shuma , Komatsu Misaki , Dong Jinhua , Ueda Hiroshi , Arai Ryoichi
Published: January 2025

Osteocalcin is a useful biomarker for bone formation and bone-related diseases. KTM219 is an anti-osteocalcin C-terminal peptide antibody. The single-chain variable region (scFv) and antigen-binding fragment (Fab) of KTM219 are applicable to the Quenchbody (Q-body) immunoassay. Q-body is a new type of fluorescent immunosensor, which is scFv or Fab labeled with a fluorescent dye. When Q-body binds to its antigen, the fluorescence intensity increases. The highly sensitive detection of antigens by changes in fluorescence intensity is performed in a single step by mixing the sample and reagent. In this study, to reveal the recognition mechanism of the KTM219 antibody and to discuss the structural basis for Q-body, we solved the crystal structures of Fab of the anti-osteocalcin antibody KTM219 and its complex with the antigen osteocalcin C-terminal peptide (BGP-C7). Also, we solved the structure of a KTM219 Fab crystal grown in the presence of a fluorescent dye, carboxytetramethylrhodamine (TAMRA); however, tightly bound TAMRA was not found in the electron density map. We predicted the binding sites of TAMRA in the antigen-binding pocket by docking simulations. These results support the proposed Q-body mechanism. The crystal structures of KTM219 Fab would be useful for further development and improvement of Q-body fluorescent immunosensors.
Don’t Be Surprised When These Surprise You: Some Infrequently Studied Sphingoid Bases, Metabolites, and Factors That Should Be Kept in Mind During Sphingolipidomic Studies
Authors: Merrill Alfred H.
Published: January 2025

Sphingolipidomic mass spectrometry has provided valuable information—and surprises—about sphingolipid structures, metabolism, and functions in normal biological processes and disease. Nonetheless, many noteworthy compounds are not routinely determined, such as the following: most of the sphingoid bases that mammals biosynthesize de novo other than sphingosine (and sometimes sphinganine) or acquire from exogenous sources; infrequently considered metabolites of sphingoid bases, such as N-(methyl)n-derivatives; “ceramides” other than the most common N-acylsphingosines; and complex sphingolipids other than sphingomyelins and simple glycosphingolipids, including glucosyl- and galactosylceramides, which are usually reported as “monohexosylceramides”. These and other subspecies are discussed, as well as some of the circumstances when they are likely to be seen (or present and missed) due to experimental conditions that can influence sphingolipid metabolism, uptake from the diet or from the microbiome, or as artifacts produced during extraction and analysis. If these compounds and factors are kept in mind during the design and interpretation of lipidomic studies, investigators are likely to be surprised by how often they appear and thereby advance knowledge about them.
Irisin and Metastatic Melanoma: Selective Anti-Invasiveness Activity in BRAF Wild-Type Cells
Authors: Serratì Simona , Zerlotin Roberta , Manganelli Michele , Di Fonte Roberta , Dicarlo Manuela , Oranger Angela , Colaianni Graziana , Porcelli Letizia , Azzariti Amalia , Guida Stefania , Grano Maria , Colucci Silvia Concetta , Guida Gabriella
Published: January 2025

Irisin is a newly discovered 12 kDa messenger protein involved in energy metabolism. Irisin affects signaling pathways in several types of cancer; however, the role of irisin in metastatic melanoma (MM) has not been described yet. We explored the biological effects of irisin in in vitro models of MM cells (HBLwt/wt, LND1wt/wt, Hmel1V600K/wt and M3V600E/V600E) capable of the oncogenic activation of BRAF. We treated MM cells with different concentrations of r-irisin (10 nM, 25 nM, 50 nM, 100 nM) for 24 h–48 h. An MTT assay highlighted that r-irisin did not affect the proliferation of MM cells. We subsequently treated MM cells with 10 nM r-irisin, corresponding to the dose exhibiting biological activity in vitro. Irisin reduced the invasive ability of only LND1wt/wt (p < 0.05), which highly expressed αv gene levels, but did not affect the invasion of BRAFmut cells. Gelatin zymography analysis showed a reduction in the enzymatic activity of MMP-2 and MMP-9 in BRAFwt/wt cells treated with 10 nM r-irisin. Moreover, gene expression analysis (qPCR) of MMP-2 and MMP-9 and of the fibrinolytic system (uPAR, uPA and PAI-1) highlighted a crucial role of 10 nM r-irisin treatment in the inhibition of pro-invasive systems in BRAFwt/wt. In conclusion, our results may suggest a possible differential role of irisin in melanoma cells.
Unraveling Effects of miRNAs Associated with APR Leaf Rust Resistance Genes in Hybrid Forms of Common Wheat (Triticum aestivum L.)
Authors: Spychała Julia , Noweiska Aleksandra , Tomkowiak Agnieszka , Bobrowska Roksana , Szewczyk Katarzyna , Kwiatek Michał Tomasz
Published: January 2025

The fungus Puccinia triticina Eriks (Pt) is the cause of leaf rust, one of the most damaging diseases, which significantly reduces common wheat yields. In Pt-resistant adult plants, an APR-type resistance is observed, which protects the plant against multiple pathogen races and is distinguished by its persistence under production conditions. With a more complete understanding of the molecular mechanisms underlying the function of APR genes, it will be possible to develop new strategies for resistance breeding in wheat. Currently, mainly APR genes, such as Lr34, Lr46, and Lr67, are principally involved in resistance breeding as they confer durable resistance to multiple fungal races occurring under different climatic and environmental conditions. However, the mechanisms underlying the defence against pathogens mediated by APR genes remain largely unknown. Our research aimed to shed light on the molecular mechanisms related to resistance genes and miRNAs expression, underlying APR resistance to leaf rust caused by Pt. Furthermore, the present study aimed to identify and functionally characterize the investigated miRNAs and their target genes in wheat in response to leaf rust inoculation. The plant material included hybrid forms of wheat from the F2 and BC1F1 generations, obtained by crossing the resistance cultivar Glenlea (CItr 17272) with agriculturally important Polish wheat cultivars. Biotic stress was induced in adult plants via inoculation with Pt fungal spores under controlled conditions. The RT-qPCR method was used to analyze the expression profiles of selected APR genes at five time points (0, 6, 12, 24, and 48 hpi). The results presented here demonstrate the differential expression of APR genes and miRNAs at stages of leaf rust development at selected timepoints after inoculation. We analyzed the expression of three leaf rust resistance genes, using different genetic backgrounds in F2 and BC1F1 segregation materials, in leaf tissues after Pt infection. Our goal was to investigate potential differences resulting from the genetic background found in different generations of hybrid forms of the same parental forms. Gene ontology analysis predicted 190 target genes for tae-miR5384-3p and 167 target genes for tae-miR9653b. Our findings revealed distinct expression profiles for genes, with the highest expression levels observed mainly at 6, 24, and 48 hpi. The candidate gene Lr46-Glu2 displayed an upregulation, suggesting its potential involvement in the immune response against Pt infection.
Outcomes of a Pilot Newborn Screening Program for Spinal Muscular Atrophy in the Valencian Community
Authors: Berzal-Serrano Alba , García-Bohórquez Belén , Aller Elena , Jaijo Teresa , Pitarch-Castellano Inmaculada , Rausell Dolores , García-García Gema , Millán José M.
Published: January 2025

Spinal muscular atrophy (SMA) is a degenerative neuromuscular condition resulting from a homozygous deletion of the survival motor neuron 1 (SMN1) gene in 95% of patients. A timely diagnosis via newborn screening (NBS) and initiating treatment before the onset of symptoms are critical for improving health outcomes in affected individuals. We carried out a screening test by quantitative PCR (qPCR) to amplify the exon seven of SMN1 using dried blood spot (DBS) samples. From October 2021 to August 2024, a total of 31,560 samples were tested in the Valencian Community (Spain) and 4 of them were positive for SMA, indicating an incidence of 1/7890. Genetic confirmation was performed using multiplex ligation-dependent probe amplification (MLPA) and AmplideX PCR/CE SMN1/2 Plus kit, in parallel obtaining concordant results in survival motor neuron 2 (SMN2) gene copy number. Within the first few weeks of their lives, two of the four patients detected by NBS showed signs of severe hypotonia, becoming ineligible for treatment. The other two patients were the first presymptomatic patients with two copies of SMN2 to receive treatment with Risdiplam in Spain. In order to treat positive cases in their early stages, we conclude that the official deployment of SMA newborn screening is necessary.
Enhancing Privacy While Preserving Context in Text Transformations by Large Language Models
Authors: Żarski Tymon Lesław , Janicki Artur
Published: January 2025

Data security is a critical concern for Internet users, primarily as more people rely on social networks and online tools daily. Despite the convenience, many users are unaware of the risks posed to their sensitive and personal data. This study addresses this issue by presenting a comprehensive solution to prevent personal data leakage using online tools. We developed a conceptual solution that enhances user privacy by identifying and anonymizing named entity classes representing sensitive data while maintaining the original context by swapping source entities for functional data. Our approach utilizes natural language processing methods, combining machine learning tools such as MITIE and spaCy with rule-based text analysis. We employed regular expressions and large language models to anonymize text, preserving its context for further processing or enabling restoration to the original form after transformations. The results demonstrate the effectiveness of our custom-trained models, achieving an F1 score of 0.8292. Additionally, the proposed algorithms successfully preserved context in approximately 93.23% of test cases, indicating a promising solution for secure data handling in online environments.
Quantifying Anisotropic Properties of Old–New Concrete Interfaces Using X-Ray Computed Tomography and Homogenization
Authors: Zhang Guanming , Lu Yang
Published: January 2025

The interface between old and new concrete is a critical component in many construction practices, including concrete pavements, bridge decks, hydraulic dams, and buildings undergoing rehabilitation. Despite various treatments to enhance bonding, this interface often remains a weak layer that compromises overall structural performance. Traditional design methods typically oversimplify the interface as a homogeneous or empirically adjusted factor, resulting in significant uncertainties. This paper introduces a novel framework for quantifying the anisotropic properties of old–new concrete interfaces using X-ray computed tomography (CT) and finite element-based numerical homogenization. The elastic coefficient matrix reveals that specimens away from the interface exhibit higher values in both normal and shear directions, with normal direction values averaging 33.15% higher and shear direction values 39.96% higher than those at the interface. A total of 10 sampling units along the interface were collected and analyzed to identify the “weakest vectors” in normal and shear directions. The “weakest vectors” at the interface show consistent orientations with an average cosine similarity of 0.62, compared with an average cosine similarity of 0.23 at the non-interface, which demonstrates directional features. Conversely, the result of average cosine similarity at the interface shows randomness that originates from the anisotropy of materials. The average angle between normal and shear stresses was found to be 88.64°, indicating a predominantly orthogonal relationship, though local stress distributions introduced slight deviations. These findings highlight the importance of understanding the anisotropic properties of old–new concrete interfaces to improve design and rehabilitation practices in concrete and structural engineering.
Nontarget Catches of Green and Brown Lacewings (Insecta: Neuroptera: Chrysopidae, Hemerobiidae) Collected by Light- and Volatile-Baited Traps in the Transcarpathian Lowland (W Ukraine)
Authors: Szanyi Kálmán , Nagy Antal , Ősz Aletta , Ábrahám Levente , Molnár Attila , Tóth Miklós , Szanyi Szabolcs
Published: January 2025

Distribution data on the lacewing fauna of the data-deficient Transcarpathian Lowland (West Ukraine) were provided. The attractivity of phenylacetaldehyde-(FLO) and isoamyl alcohol-based (SBL) lures designed for trapping lepidopteran pests to lacewings was also studied and compared to the efficiency of light traps traditionally used in studies on neuropterans. In the three-year study, 374 individuals of 10 species were caught. Although the light trap was the most efficient method, the efficiency of the tested lures could also be proved. Regarding abundances, FLO was significantly more efficient than the SBL lure. The lures could supplement the checklist of the fauna with two species and attracted an especially high number of Chrysoperla species. In the case of parallel use with light traps, they serve as an efficient standardised combined method for trapping lacewings, both in faunistic studies and plant protection applications.
The Utilization of Inert Materials for the Control of Stored-Product Mites—A Mini Review
Authors: Athanassiou Christos G. , Rumbos Christos I. , Agrafioti Paraskevi , Sakka Maria K.
Published: January 2025

Stored-product mites are important pests of stored products, while their presence in storage and processing facilities has a significant effect on public health. On the other hand, inert materials are promising alternatives to conventional pesticides in stored product protection and have provided very good results against storage insects. These formulations can be applied either directly on the product or on surfaces, as dusts or as slurry formulations. In the current paper, we review the factors that affect the efficacy of inert dusts, emphasizing in diatomaceous earths, against stored-product mites. Hence, we address the different biotic and abiotic factors that affect the acaricidal effect of inert materials against different mite species, but also the complexity of such an application, that often arises from the simultaneous presence of plant-feeding mites with their mite predators. Finally, we provide some insights for further research directions.
Relationship Between Poor Sleep and Depressive Symptoms in Korean Elite Youth Athletes
Authors: Choi Youngju , Kim Seoyeon , Park Soo-Hyun , Kim Kitae , Kim Rye Kyeong , Kwak Hyo-Bum , Cho Jinkyung
Published: January 2025

Background: Sleep and mental health are crucial to elite youth athletes, who face combined pressures of training, competition, school, and social commitments. We examined the association between sleep and depression in elite youth athletes. Methods: We analyzed data of 248 Korean world-class youth athletes (aged 13–19 years). The athletes completed the Pittsburgh Sleep Quality Index (PSQI), the Morningness–Eveningness Questionnaire (MEQ), and the Center for Epidemiologic Studies Depression Scale (CES-D). Results: Overall, 50.4% of the athletes were considered poor sleepers (global PSQI score ≥ 5.5); 23.8% reported depressive symptoms (CES-D score ≥ 16). Depression was 4.26 times (95% confidence interval [CI], 2.00–9.09, p < 0.001) more likely in poor than in good sleepers. Odds of depression were increased with poor subjective sleep quality (odds ratio [OR]: 4.62; 95%CI, 2.10–10.18, p < 0.001), prolonged sleep latency (OR: 2.45: 95% CI, 1.28–4.69, p < 0.01), increased sleep disturbances (OR: 3.98: 95% CI, 1.83–8.63, p < 0.001), and daytime dysfunction (OR: 3.28; 95% CI, 1.67–6.44, p < 0.001). Depressive symptoms were associated with worse sleep, particularly poor subjective sleep quality, prolonged sleep latency, increased sleep disturbances, and increased daytime dysfunction. Conclusions: These results suggest that depressive symptoms are associated with poor sleep in the elite youth athlete population.
A First Randomized Eight-Week Multidisciplinary Telerehabilitation Study for the Post-COVID-19 Condition: Improvements in Health- and Pain-Related Parameters
Authors: Bileviciute-Ljungar Indre , Apelman Agneta , Braconier Lena , Östhols Sara , Norrefalk Jan-Rickard , Borg Kristian
Published: January 2025

Background/Objectives: This study investigates the effects of multidisciplinary telerehabilitation for the post-COVID-19 condition. Methods: Recruitment was announced during 2021/22 through the COVID patient organisation in Sweden. The key inclusion criteria were persistent symptoms and functional impairments longer than 12 weeks after an acute SARS-CoV-2 infection, a stable health condition, and satisfactory skills to use the Internet. Participants were randomised into a rehabilitation group or a waiting list. Telerehabilitation was performed by a multidisciplinary team. Measured outcomes included health- and pain-related parameters and pain-related disability after eight weeks and when followed up after six months. Results: In total, 164 participants registered for the study. Of them, 67 participated in an eight-week group telerehabilitation programme (mean age 43, 78% women) and 42 stayed on a waiting list (mean age 47, 88% women) after eight weeks. Sixty participants from the rehabilitation group and 21 from the waiting list completed the data at the six-month follow-up. The results show greater improvements in both health- and pain-related parameters within the telerehabilitation group after eight weeks and six months than within the waiting list group (Cohen’s d effect size varied between moderate and large). However, no statistically significant differences were found between the groups, except participants being at a lower risk of anxiety measured based on the Hospital Anxiety and Depression Scale after eight weeks of telerehabilitation compared to those on the waiting list. Conclusions: The results indicate that multidisciplinary group telerehabilitation is beneficial for improving health- and pain-related parameters in people suffering from the post-COVID-19 condition and should be further developed and adapted for similar conditions, such as chronic pain, fatigue, etc.