bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrative analysis of metabolome and transcriptome reveals the mechanism of color formation in cassava (Manihot esculenta Crantz) leaves
Authors: Luo Xiuqin , An Feifei , Xue Jingjing , Zhu Wenli , Wei Zhuowen , Ou Wenjun , Li Kaimian , Chen Songbi , Cai Jie
Published: June 2023

Cassava (Manihot esculenta Crantz) leaves are often used as vegetables in Africa. Anthocyanins possess antioxidant, anti-inflammatory, anti-cancer, and other biological activities. They are poor in green leaves but rich in the purple leaves of cassava. The mechanism of anthocyanin’s accumulation in cassava is poorly understood. In this study, two cassava varieties, SC9 with green leaves and Ziyehuangxin with purple leaves (PL), were selected to perform an integrative analysis using metabolomics and transcriptomics. The metabolomic analysis indicated that the most significantly differential metabolites (SDMs) belong to anthocyanins and are highly accumulated in PL. The transcriptomic analysis revealed that differentially expressed genes (DEGs) are enriched in secondary metabolites biosynthesis. The analysis of the combination of metabolomics and transcriptomics showed that metabolite changes are associated with the gene expressions in the anthocyanin biosynthesis pathway. In addition, some transcription factors (TFs) may be involved in anthocyanin biosynthesis. To further investigate the correlation between anthocyanin accumulation and color formation in cassava leaves, the virus-induced gene silencing (VIGS) system was used. VIGS-MeANR silenced plant showed the altered phenotypes of cassava leaves, partially from green to purple color, resulting in a significant increase of the total anthocyanin content and reduction in the expression of MeANR. These results provide a theoretical basis for breeding cassava varieties with anthocyanin-rich leaves.
An experimental investigation into scope rigidity in written Mandarin
Authors: Wu Hongchen
Published: June 2023

Mandarin Chinese is claimed to be a scope-rigid language, as its doubly-quantified simple transitive sentences are unambiguous with surface scope only and no inverse scope available. However, it has been debated whether Mandarin Chinese allows inverse scope in some syntactic environments other than simple transitives. This paper investigates whether scope rigidity as a property of the grammar of Mandarin prevents scope ambiguity in different syntactic environments and what factors influence scope interpretations. Using a Truth-Value Judgment task, we tested the judgments of 98 Mandarin Chinese native speakers on transitive sentences containing both a subject and object quantifier under adverbial clauses. The results show that inverse scope reading is considered available for doubly-quantified transitives under adverbial clauses, although there are intra-participant variances. The results challenge the well-established approaches to quantifier scope in Mandarin and call for rethinking the long-standing dichotomy view of quantifier scope in languages. We also found bimodal distribution on the acceptance of inverse scope readings, suggesting that there may be two different populations of native speakers with two different grammars. In addition, we also observed other factors that may affect scope behaviors, including clause type, presence of aspect marker, verb type, and numbers.
Identifying factors influencing COVID-19 vaccine uptake in Finland – a qualitative study using social media data
Authors: Lohiniva Anna-Leena , Pensola Annika , Hyökki Suvi , Sivelä Jonas , Härmä Vuokko , Tammi Tuukka
Published: June 2023

Introduction

Vaccine demand creation requires understanding what is driving the uptake of the vaccine. 24 Qualitative research methods are paramount to gaining a localized understanding of behavioral 25 drivers and barriers to vaccine uptake, but they are often underutilized.

Methods

This is a qualitative study that 26 used public comments on the Facebook and Twitter posts of the Finnish Institute for Health and 27 Welfare (THL) as data sources to identify behavioral drivers for COVID-19 vaccine uptake in 28 Finland. The participatory data analysis utilized thematic analysis and the Theoretical Domains 29 Framework (TDF). NVIVO was used to assist in the coding process.

Results

The greatest number of FB and 30 Twitter comments were linked with six TDF domains: knowledge, environmental context and 31 resources, beliefs in consequences, beliefs in capabilities, social and professional role, and social 32 influences. The domains included 15 themes that were interlinked. The knowledge domain 33 overlapped with all other domains.

Discussion

By using public discourse on Facebook and Twitter, and rapid 34 qualitative data analysis methods within a behavioral insight framework, this study adds to the 35 emerging knowledge about behavioral drivers of COVID-19 vaccines that can be used by public 36 health experts to enhance the uptake of vaccines during future pandemics and epidemics.
Adolescents with disabilities and caregivers experience of COVID-19 in rural Nepal
Authors: Morrison Joanna , Poudyal Niraj , Pun Insha , Prasai Sagar , Shrestha Nir , Khadka Dipesh , Shrestha Sushmita , Rohwerder Brigitte , Wickenden Mary
Published: June 2023

Introduction

Intersecting vulnerabilities of disability, low socio-economic status, marginalization, and age indicate that adolescents with disabilities in low-and middle-income countries were uniquely affected by the COVID-19 pandemic. Yet, there has been limited research about their experience. We conducted participatory research with adolescents with disabilities in rural, hilly Nepal to explore their experience of the pandemic and inform understanding about how they can be supported in future pandemics and humanitarian emergencies.

Methods

We used qualitative methods, purposively sampling adolescents with different severe impairments from two rural, hilly areas of Nepal. We collected data through semi-structured interviews with five girls and seven boys between the age of 11 and 17 years old. Interviews used inclusive, participatory, and arts-based methods to engage adolescents, support discussions and enable them to choose what they would like to discuss. We also conducted semi-structured interviews with 11 caregivers.

Results

We found that adolescents with disabilities and their families experienced social exclusion and social isolation because of COVID-19 mitigation measures, and some experienced social stigma due to misconceptions about transmission of COVID-19 and perceived increased vulnerability of adolescents with disabilities to COVID-19. Adolescents who remained connected with their peers throughout lockdown had a more positive experience of the pandemic than those who were isolated from friends. They became disconnected because they moved away from those they could communicate with, or they had moved to live with relatives who lived in a remote, rural area. We found that caregivers were particularly fearful and anxious about accessing health care if the adolescent they cared for became ill. Caregivers also worried about protecting adolescents from COVID-19 if they themselves got ill, and about the likelihood that the adolescent would be neglected if the caregiver died.

Conclusion

Contextually specific research with adolescents with disabilities to explore their experience of the pandemic is necessary to capture how intersecting vulnerabilities can adversely affect particular groups, such as those with disabilities. The participation of adolescents with disabilities and their caregivers in the development of stigma mitigation initiatives and strategies to meet their needs in future emergencies is necessary to enable an informed and inclusive response.
Do women's empowerment and self-expression values change adolescents' gendered occupational expectations? Longitudinal evidence against the gender-equality paradox from 26 European countries
Authors: Erdmann Melinda , Marques Hill Agustina , Helbig Marcel , Leuze Kathrin
Published: June 2023

Despite the increases in women's educational attainment in recent decades, female labor market participation and labor market returns are still lower than those of their male counterparts. Among the main factors explaining this persistence of economic inequality is the persistently gendered nature of occupational expectations, which results in gender segregation of labor. In this paper, we describe how gender-specific adolescents' occupational expectations change over time (2006–2018) and how women's empowerment and cultural norms might influence gender-specific occupational expectations. Against the backdrop of the research on the gender-equality paradox and from a comparative perspective, we focus on national and institutional characteristics to investigate how individual and national factors explain gendered occupational expectations. We answer our research questions by applying a two-step multilevel model with fixed effects. For this, we used PISA data and merged them with state-level information from 26 European countries. We add to existing research by making three contributions. First, we describe the changes in occupational expectations over time within European countries by looking at the gender composition of the desired occupation and distinguishing three categories (gender-typical, gender-balanced, and gender-atypical). Second, we investigate the relationship between national characteristics and the evolution of gendered occupational expectations separately by gender to reveal gender-specific mechanisms. Third, by using data from two-time points, we explore which national-level changes lead to changes in students' occupational expectations. Our first descriptive results show that the patterns of how students' occupational expectations change over time differ remarkably between countries. In 2018 in some countries, students' occupational expectations became more segregated while in others the number of students with gender-balanced or gender-atypical expectations increased. Our fixed effects models show that women's empowerment and self-expression value explained variance over time. For example, women's empowerment measured via an increase in women's employment and participation in parliament led to less gender-typical occupational expectations among girls and boys. Similarly, a rise in self-expression values led to less gender-typical occupational expectations, again for both boys and girls. Remarkably, our results do not verify the gender-equality paradox for occupational expectations, as is the case in previous cross-sectional analyses.
Molecular characterization of Fasciola hepatica in endemic regions of Colombia
Authors: Garcia-Corredor Diego , Alvarado Mateo , Pulido-Medellín Martín , Muñoz Marina , Cruz-Saavedra Lissa , Hernández Carolina , Giraldo Julio Cesar , Vásquez-Arteaga Luis R. , Morillo Coronado Ana Cruz , Ramírez Juan David
Published: June 2023

Fasciola hepaticais a zoonotic trematode that affects a wide range of hosts, including cattle, sheep, and goats. The economic impact of the parasite on the cattle industry is significant, with high losses reported worldwide. While its impact on human health was previously underestimated, recent years have seen a rise in fascioliasis cases, leading to increased interest among researchers globally. To characterize the genetic diversity and intraspecific variation of this parasite in South America, specifically in Colombia, we collected 105 adult parasites from cattle bile ducts in seven Colombian departments (Antioquia, Boyacá, Santander, Cauca, Cundinamarca, Nariño, Norte de Santander, and Santander) to assess the parasite’s phenotypic analyses, genetic diversity, and population structure. A computer image analysis system (CIAS) was applied based on standardized morphological measurements. Liver-fluke size was studied by principal component analysis (PCA). DNA sequences were obtained for nuclear markers such as the 28S, β-tubulin 3, ITS1, ITS2, and the mitochondrial marker Cytochrome Oxidase I (COI). Multiple statistical tests were performed, and the parasite’s population structure was analyzed. Maximum Likelihood (ML) phylogenetic reconstructions were carried out using the sequences obtained herein and sequences available in GenBank. Morphological results revealed that all the obtained individuals matchedF. hepatica’s morphology. There was no evidence of high genetic diversity, and the absence of genetic structure at the country-level was notable, possibly caused by a demographic expansion of this trematode in Colombia or the low resolution of the molecular markers employed. Future studies are still needed to unveil the genetic population structure ofF. hepaticaacross the country.
Evaluation of SARS-CoV-2 passive surveillance in Lithuanian mink farms, 2020–2021
Authors: Žigaitė Silvija , Masiulis Marius , Bušauskas Paulius , Pilevičienė Simona , Buitkuvienė Jūratė , Paulauskas Vidmantas , Malakauskas Alvydas
Published: June 2023

The newly emerged SARS-CoV-2, causing COVID-19 in humans, is also infecting American mink (Neovison vison), used in fur production. Since 2020, passive surveillance of SARS-CoV-2 in mink farms was implemented in Lithuania. Here, we describe data from a survey of all 57 active Lithuanian mink farms carried out during November–December 2021 to complement passive surveillance in the country. In all 57 mink farms, nasopharyngeal swab samples were collected from dead or live mink and tested by real-time RT-PCR. Dead mink samples were tested in pools of 5, while live mink samples were tested individually. In 19 mink farms, blood serum was collected and tested for antibodies to determine previous exposure to the virus. Environmental samples from 55 farms were also collected and tested in pooled samples by real-time RT-PCR. The present survey has detected 22.81% viral RNA-positive mink farms and a high number of mink farms that were exposed (84.21, 95% CI 67.81–100%) to the virus. The increasing exposure of mink farms to the virus due to growing human COVID-19 cases and limitations of passive surveillance could explain the observed epidemiological situation of SARS-CoV-2 in Lithuanian mink farms, compared to the few positive farms previously detected by passive surveillance. The unexpected widespread exposure of mink farms to SARS-CoV-2 suggests that passive surveillance is ineffective for early detection of SARS-CoV-2 in mink. Further studies are needed to reveal the present status in previously infected mink farms.
Tumor subtypes and signature model construction based on chromatin regulators for better prediction of prognosis in uveal melanoma
Authors: Li Yue , Xiong Chao , Wu Li Li , Zhang Bo Yuan , Wu Sha , Chen Yu Fen , Xu Qi Hua , Liao Hong Fei
Published: June 2023

Background: Uveal Melanoma (UM) is the most prevalent primary intraocular malignancy in adults. This study assessed the importance of chromatin regulators (CRs) in UM and developed a model to predict UM prognosis.

Methods: Gene expression data and clinical information for UM were obtained from public databases. Samples were typed according to the gene expression of CRs associated with UM prognosis. The prognostic key genes were further screened by the protein interaction network, and the risk model was to predict UM prognosis using the least absolute shrinkage and selection operator (LASSO) regression analysis and performed a test of the risk mode. In addition, we performed gene set variation analysis, tumor microenvironment, and tumor immune analysis between subtypes and risk groups to explore the mechanisms influencing the development of UM.

Results: We constructed a signature model consisting of three CRs (RUVBL1, SIRT3, and SMARCD3), which was shown to be accurate, and valid for predicting prognostic outcomes in UM. Higher immune cell infiltration in poor prognostic subtypes and risk groups. The Tumor immune analysis and Tumor Immune Dysfunction and Exclusion (TIDE) score provided a basis for clinical immunotherapy in UM.

Conclusion: The risk model has prognostic value for UM survival and provides new insights into the treatment of UM.
Phenotype and Reactivity of Lymphocytes Expanded from Benign Prostate Hyperplasic Tissues and Prostate Cancer
Authors: Ahmed Ritaparna , Lozano Leyder Elena , Anastasio Amandine , Lofek Sebastien , Mastelic-Gavillet Beatris , Navarro Rodrigo Blanca , Nguyen Sylvain , Dartiguenave Florence , Rodrigues-Dias Sonia-Cristina , Cesson Valérie , Valério Massimo , Roth Beat , Kandalaft Lana Elias , Redchenko Irina , Hill Adrian Vivian Sinton , Harari Alexandre , Romero Pedro , Derré Laurent , Viganó Selena
Published: June 2023

Benign prostate hyperplasia (BPH) is a frequent condition in aging men, which affects life quality, causing principally lower urinary tract symptoms. Epidemiologic studies suggest that BPH may raise the risk of developing prostate cancer (PCa), most likely promoting a chronic inflammatory environment. Studies aiming at elucidating the link and risk factors that connect BPH and PCa are urgently needed to develop prevention strategies. The BPH microenvironment, similar to the PCa one, increases immune infiltration of the prostate, but, in contrast to PCa, immunosuppression may not be established yet. In this study, we found that prostate-infiltrating lymphocytes (PILs) expanded from hyperplastic prostate tissue recognized tumor-associated antigens (TAA) and autologous tissue, regardless of the presence of tumor cells. PILs expanded from BPH samples of patients with PCa, however, seem to respond more strongly to autologous tissue. Phenotypic characterization of the infiltrating PILs revealed a trend towards better expanding CD4+ T cells in infiltrates derived from PCa, but no significant differences were found. These findings suggest that T cell tolerance is compromised in BPH-affected prostates, likely due to qualitative or quantitative alterations of the antigenic landscape. Our data support the hypothesis that BPH increases the risk of PCa and may pave the way for new personalized preventive vaccine strategies for these patients.
UAS™—A Urine Preservative for Oncology Applications
Authors: Jordaens Stephanie , Arora Amit , MacDonald Kyle W. , Wood Cameron , Hendrickx Jhana O. , Zwaenepoel Karen , Deben Christophe , Tjalma Wiebren , Pauwels Patrick , Beyers Koen , Vankerckhoven Vanessa
Published: June 2023

Liquid biopsy is a revolutionary tool that is gaining momentum in the field of cancer research. As a body fluid, urine can be used in non-invasive diagnostics for various types of cancer. We investigated the performance of UAS™ as a preservative for urinary analytes. Firstly, the need for urine preservation was investigated using urine samples from healthy volunteers. Secondly, the performance of UAS™ was assessed for cell-free DNA (cfDNA) and host cell integrity during storage at room temperature (RT) and after freeze-thaw cycling. Finally, UAS™ was used in a clinical setting on samples from breast and prostate cancer patients. In the absence of a preservative, urinary cfDNA was degraded, and bacterial overgrowth occurred at RT. In urine samples stored in UAS™, no microbial growth was seen, and cfDNA and cellular integrity were maintained for up to 14 days at RT. After freeze-thaw cycling, the preservation of host cell integrity and cfDNA showed significant improvements when using UAS™ compared to unpreserved urine samples. Additionally, UAS™ was found to be compatible with several commercially available isolation methods.
‘Earlier than Early’ Detection of Breast Cancer in Israeli BRCA Mutation Carriers Applying AI-Based Analysis to Consecutive MRI Scans
Authors: Anaby Debbie , Shavin David , Zimmerman-Moreno Gali , Nissan Noam , Friedman Eitan , Sklair-Levy Miri
Published: June 2023

Female BRCA1/BRCA2 (=BRCA) pathogenic variants (PVs) carriers are at a substantially higher risk for developing breast cancer (BC) compared with the average risk population. Detection of BC at an early stage significantly improves prognosis. To facilitate early BC detection, a surveillance scheme is offered to BRCA PV carriers from age 25–30 years that includes annual MRI based breast imaging. Indeed, adherence to the recommended scheme has been shown to be associated with earlier disease stages at BC diagnosis, more in-situ pathology, smaller tumors, and less axillary involvement. While MRI is the most sensitive modality for BC detection in BRCA PV carriers, there are a significant number of overlooked or misinterpreted radiological lesions (mostly enhancing foci), leading to a delayed BC diagnosis at a more advanced stage. In this study we developed an artificial intelligence (AI)-network, aimed at a more accurate classification of enhancing foci, in MRIs of BRCA PV carriers, thus reducing false-negative interpretations. Retrospectively identified foci in prior MRIs that were either diagnosed as BC or benign/normal in a subsequent MRI were manually segmented and served as input for a convolutional network architecture. The model was successful in classification of 65% of the cancerous foci, most of them triple-negative BC. If validated, applying this scheme routinely may facilitate ‘earlier than early’ BC diagnosis in BRCA PV carriers.
Reduction of Tumor Growth with RNA-Targeting Treatment of the NAB2–STAT6 Fusion Transcript in Solitary Fibrous Tumor Models
Authors: Li Yi , Nguyen John T. , Ammanamanchi Manasvini , Zhou Zikun , Harbut Elijah F. , Mondaza-Hernandez Jose L. , Meyer Clark A. , Moura David S. , Martin-Broto Javier , Hayenga Heather N. , Bleris Leonidas
Published: June 2023

Solitary fibrous tumor (SFT) is a rare soft-tissue sarcoma. This nonhereditary cancer is the result of an environmental intrachromosomal gene fusion between NAB2 and STAT6 on chromosome 12, which fuses the activation domain of STAT6 with the repression domain of NAB2. Currently there is not an approved chemotherapy regimen for SFTs. The best response on available pharmaceuticals is a partial response or stable disease for several months. The purpose of this study is to investigate the potential of RNA-based therapies for the treatment of SFTs. Specifically, in vitro SFT cell models were engineered to harbor the characteristic NAB2–STAT6 fusion using the CRISPR/SpCas9 system. Cell migration as well as multiple cancer-related signaling pathways were increased in the engineered cells as compared to the fusion-absent parent cells. The SFT cell models were then used for evaluating the targeting efficacies of NAB2–STAT6 fusion-specific antisense oligonucleotides (ASOs) and CRISPR/CasRx systems. Our results showed that fusion specific ASO treatments caused a 58% reduction in expression of fusion transcripts and a 22% reduction in cell proliferation after 72 h in vitro. Similarly, the AAV2-mediated CRISPR/CasRx system led to a 59% reduction in fusion transcript expressions in vitro, and a 55% reduction in xenograft growth after 29 days ex vivo.
Immuno-PET Imaging of Tumour PD-L1 Expression in Glioblastoma
Authors: Sharma Gitanjali , Braga Marta C. , Da Pieve Chiara , Szopa Wojciech , Starzetz Tatjana , Plate Karl H. , Kaspera Wojciech , Kramer-Marek Gabriela
Published: June 2023

There is no established method to assess the PD-L1 expression in brain tumours. Therefore, we investigated the suitability of affibody molecule (ZPD-L1) radiolabelled with F-18 (Al18F) and Ga-68 to measure the expression of PD-L1 in xenograft mouse models of GBM. Mice bearing subcutaneous and orthotopic tumours were imaged 1 h post-radioconjugate administration. Ex vivo biodistribution studies and immunohistochemistry (IHC) staining were performed. Tumoural PD-L1 expression and CD4+/CD8+ tumour-infiltrating lymphocytes were evaluated in human GBM specimens. ZPD-L1 was radiolabelled with radiochemical yields of 32.2 ± 4.4% (F-18) and 73.3 ± 1.8% (Ga-68). The cell-associated radioactivity in vitro was consistent with PD-L1 expression levels assessed with flow cytometry. In vivo imaging demonstrated that 18F-AlF-NOTA-ZPD-L1 can distinguish between PD-L1 high-expressing tumours (U87-MGvIII) and PD-L1-negative ones (H292PD-L1Ko). The radioconjugate was quickly cleared from the blood and normal tissues, allowing for high-contrast images of brain tumours as early as 1 h post-injection. 68Ga-NOTA-ZPD-L1 showed heterogeneous and diffuse accumulation that corresponded to the extensively infiltrating GCGR-E55 tumours involving contiguous lobes of the brain. Lastly, 39% of analysed GBM patient samples showed PD-L1+ staining of tumour cells that was associated with elevated levels of CD4+ and CD8+ lymphocytes. Our results suggest that the investigated radioconjugates are very promising agents with the potential to facilitate the future design of treatment regimens for GBM patients.
Organoids as Innovative Models for Bone and Joint Diseases
Authors: Huang Jie , Zhang Lingqiang , Lu Aiping , Liang Chao
Published: June 2023

Bone is one of the key components of the musculoskeletal system. Bone and joint disease are the fourth most widespread disease, in addition to cardiovascular disease, cancer, and diabetes, which seriously affect people’s quality of life. Bone organoids seem to be a great model by which to promote the research method, which further could improve the treatment of bone and joint disease in the future. Here, we introduce the various bone and joint diseases and their biology, and the conditions of organoid culture, comparing the in vitro models among 2D, 3D, and organoids. We summarize the differing potential methods for culturing bone-related organoids from pluripotent stem cells, adult stem cells, or progenitor cells, and discuss the current and promising bone disease organoids for drug screening and precision medicine. Lastly, we discuss the challenges and difficulties encountered in the application of bone organoids and look to the future in order to present potential methods via which bone organoids might advance organoid construction and application.
SIRT1 Activator E1231 Alleviates Nonalcoholic Fatty Liver Disease by Regulating Lipid Metabolism
Authors: Han Jiangxue , Li Shunwang , Wang Weizhi , Jiang Xinhai , Liu Chao , Lei Lijuan , Li Yining , Sheng Ren , Zhang Yuyan , Wu Yexiang , Zhang Jing , Zhang Yuhao , Xu Yanni , Si Shuyi
Published: June 2023

Nonalcoholic fatty liver disease (NAFLD) is one of the most common liver diseases. Silencing information regulator 1 (SIRT1) was demonstrated to modulate cholesterol and lipid metabolism in NAFLD. Here, a novel SIRT1 activator, E1231, was studied for its potential improvement effects on NAFLD. C57BL/6J mice were fed a high-fat and high-cholesterol diet (HFHC) for 40 weeks to create a NAFLD mouse model, and E1231 was administered by oral gavage (50 mg/kg body weight, once/day) for 4 weeks. Liver-related plasma biochemistry parameter tests, Oil Red O staining, and hematoxylin-eosin staining results showed that E1231 treatment ameliorated plasma dyslipidemia, plasma marker levels of liver damage (alanine aminotransferase (ALT) and aspartate aminotransferase (AST)), liver total cholesterol (TC) and triglycerides (TG) contents, and obviously decreased hepatic steatosis score and NAFLD Activity Score (NAS) in the NAFLD mouse model. Western blot results showed that E1231 treatment significantly regulated lipid-metabolism-related protein expression. In particular, E1231 treatment increased SIRT1, PGC-1α, and p-AMPKα protein expression but decreased ACC and SCD-1 protein expression. Additionally, in vitro studies demonstrated that E1231 inhibited lipid accumulation and improved mitochondrial function in free-fatty-acid-challenged hepatocytes, and required SIRT1 activation. In conclusion, this study illustrated that the SIRT1 activator E1231 alleviated HFHC-induced NAFLD development and improved liver injury by regulating the SIRT1-AMPKα pathway, and might be a promising candidate compound for NAFLD treatment.
Modeling and Pile-Driven Scaled Tests for Windfarm Foundations
Authors: Soriano Vicedo Jorge , García Barba Javier , Cobelo William Daniel , Fernández Aldo
Published: June 2023

One of the main problems associated with the generation of wind energy in offshore wind platforms is the analysis of the behavior of the soil when the pile is driven into the seabed. Nowadays, due to the large dimensions of the foundations (hollow steel piles up to 8 m in diameter and 15–20 cm thickness), there are no scale studies carried out that analyze the mechanical and deformational behavior of soil where piles are driven as well as the material of the pile that supports the motor. This paper presents the results obtained from scale submerged tests in a steel pool to analyze the behavior of sand in the presence of water where piles were installed. These tests use a hydraulic press to carry out the penetration of the steel tube in the sand. The results were compared with three different speeds for three tubes with different diameters and two types of termination at the end of the tested element. The results of the submerged tests were compared with the tests in dry conditions and with the results obtained through the finite element Plaxis program.
Health Workers’ Burnout and COVID-19 Pandemic: 1-Year after—Results from a Repeated Cross-Sectional Survey
Authors: Gambaro Eleonora , Gramaglia Carla , Marangon Debora , Probo Manuela , Rudoni Marco , Zeppegno Patrizia
Published: June 2023

(1) Background: This study evaluates, one year later, the levels of burnout, anxious–depressive, and post-traumatic symptoms and the general health status in the Health Workers (HWs) involved in the SARS-COVID-19 pandemic in the Novara area. (2) Methods: The survey was sent via a link in an email to doctors, nurses, and other operators during the period between June and August 2021. The survey collected socio-demographic data and contained some self-administered questionnaires. (3) Results: A total of 688 HWs completed the survey, 53% were aged 30–49 years, 68% were female, 76% were cohabiting, 55% had children, 86% reported family habit changes, and 20% had non-COVID related health problems. Only a few of the respondents had a follow-up by a specialist (12%), of which there were even less in recent times (6%). It was observed that the respondents had undergone burnout; a poor state of general mental health (62%); depressive symptoms (70%); post-traumatic symptoms (29%); and less frequently, anxious symptoms (16%). The data of this study are in line with other studies in the literature. (4) Conclusions: The data indicate that psychological-based suffering was no longer markedly concentrated in some specific bands of HWs. In conclusion, it would be essential to enhance HW support strategies.
Mental Health Recovery in Social Psychiatric Policies: A Reflexive Thematic Analysis
Authors: Nielsen Jacob M. , Buus Niels , Berring Lene L.
Published: June 2023

The realisation of recovery as an overarching goal of mental health care services has proven difficult to achieve in practice. At present, concepts of recovery are contested and unclear, which affects their implementation in psychiatric practices. We examined social psychiatric policies about recovery with the aim to explore their underlying assumptions about recovery. Relevant texts from the policies’ knowledge bases were subjected to reflexive thematic analysis. We developed a central theme: “A clinical standardisation of the concept of recovery”. The theme involved meaning clusters that encompassed conflicting and commonly shared assumptions about recovery across the text corpus. We discussed the findings from discourse analytical and governmentality perspectives. In conclusion, the policies’ aim of providing clarity about recovery was circumvented by the very knowledge bases used to support their endeavours.
A Summary of Concepts, Procedures and Techniques Used by Forensic Entomologists and Proxies
Authors: Bambaradeniya Tharindu B. , Magni Paola A. , Dadour Ian R.
Published: June 2023

Forensic entomology is a branch of forensic science that incorporates insects as a part of solving crime. Insect-based evidence recovered at a crime scene can be used to estimate the minimum postmortem interval, determine if a carcass/corpse has been relocated, and contribute to the cause and manner of death. The current review summarises the stepwise usage of forensic entomology methods at a crime scene and in the laboratory, including specimen collection and rearing, identification, xenobiotic detection, documentation, and referencing previous research and casework. It also provides three standards for the collection of insects when attending a crime scene. The Gold standard attributes to a forensic entomologist (FE) who is likely to be well-trained attending a scene. The subsequent standards (Silver and Bronze) have been added because the authors believe that this information is currently missing in the literature. The purpose is so that an attending crime scene agent/proxy with some basic knowledge and some simple tools can recover almost all the insect information required by an FE to make the best estimation of the minimum postmortem interval.
The U.S. Experience in Drafting Guidelines for Judicial Interviews of Children and Its Translation to Hague Abduction Convention Return Proceedings Globally
Authors: Kucinski Melissa Ann
Published: June 2023

This article will focus on judicial interviews of children, in chambers, including in Hague Abduction Convention cases; the potential promise and pitfalls of conducting such interviews; and how the U.S. experience provides an excellent template for future discussions and work on creating a soft law instrument on this important information-gathering tool.
Integrated Computing Accelerates Design and Performance Control of New Maraging Steels
Authors: Chen Shixing , Zhu Jingchuan , Liu Tingyao , Liu Yong , Fu Yudong , Shimada Toshihiro , Liu Guanqi
Published: June 2023

This paper mainly used database technology, machine learning, thermodynamic calculation, experimental verification, etc., on integrated computational materials engineering. The interaction between different alloying elements and the strengthening effect of precipitated phases were investigated mainly for martensitic ageing steels. Modelling and parameter optimization were performed by machine learning, and the highest prediction accuracy was 98.58%. We investigated the influence of composition fluctuation on performance and correlation tests to analyze the influence of elements from multiple perspectives. Furthermore, we screened out the three-component composition process parameters with composition and performance with high contrast. Thermodynamic calculations studied the effect of alloying element content on the nano-precipitation phase, Laves phase, and austenite in the material. The heat treatment process parameters of the new steel grade were also developed based on the phase diagram. A new type of martensitic ageing steel was prepared by selected vacuum arc melting. The sample with the highest overall mechanical properties had a yield strength of 1887 MPa, a tensile strength of 1907 MPa, and a hardness of 58 HRC. The sample with the highest plasticity had an elongation of 7.8%. The machine learning process for the accelerated design of new ultra-high tensile steels was found to be generalizable and reliable.
The Combined Spectral Response of a MEMS Metamaterial Absorber for the Mid-IR and Its Sub-Wavelength Fabrication Residual Array of Holes
Authors: Wolffenbuttel Reinoud F. , Ghaderi M. Amir
Published: June 2023

Metasurface coatings on a free-standing SiN thin film membrane are fabricated on a Si substrate using masked lithography and CMOS-compatible surface micromachining. The result is a band-limited absorber for the mid-IR, which is part of a microstructure that is attached to the substrate by long and slender suspension beams to provide thermal isolation. As a residual of the fabrication, the regular pattern of sub-wavelength unit cells of 2.6 μm side length, which defines the metasurface, is interrupted by an equally regular array of sub-wavelength holes of 1–2 μm diameter and at 7.8–15.6 μm of pitch. This array of holes is essential for enabling access of the etchant and attack of the underlying layer during fabrication, which ultimately results in the sacrificial release of the membrane from the underlying substrate. As the plasmonic responses of the two patterns interfere, a maximum is imposed on the hole diameter and a minimum on the hole-to-hole pitch. However, the hole diameter should be sufficiently large to allow access of the etchant, while the maximum spacing between holes is set by the limited selectivity of the different materials to the etchant during sacrificial release. The effect of the parasitic hole pattern on the spectral absorption of a metasurface design is analyzed by simulations of the responses of combined holes–metasurface structures. Arrays of 300 × 180 μm2 Al-Al2O3-Al MIM structures are mask-fabricated on suspended SiN beams. The results show that the effect of the array of holes can be disregarded for a hole-to-hole pitch larger than 6 times the side length of the metamaterial until cell, while the diameter of the hole should remain smaller than about 1.5 μm, and their alignment is critical.
Nanostructures on Fluoropolymer Nanotextile Prepared Using a High-Energy Excimer Laser
Authors: Slepička Petr , Slepičková Kasálková Nikola , Fajstavr Dominik , Frýdlová Bára , Sajdl Petr , Kolská Zdeňka , Švorčík Václav
Published: June 2023

This study is focused on polytetrafluoroethylene (PTFE) porous nanotextile and its modification with thin, silver sputtered nanolayers, combined with a subsequent modification with an excimer laser. The KrF excimer laser was set to single-shot pulse mode. Subsequently, the physico chemical properties, morphology, surface chemistry, and wettability were determined. Minor effects of the excimer laser on the pristine PTFE substrate were described, but significant changes were observed after the application of the excimer laser to the polytetrafluoroethylene with sputtered silver, where the formation of a silver nanoparticles/PTFE/Ag composite was described, with a wettability similar to that of a superhydrophobic surface. Both scanning electron microscopy and atomic force microscopy revealed the formation of superposed globular structures on the polytetrafluoroethylene lamellar primary structure, which was also confirmed using energy dispersive spectroscopy. The combined changes in the surface morphology, chemistry, and thus wettability induced a significant change in the PTFE’s antibacterial properties. Samples coated with silver and further treated with the excimer laser 150 mJ/cm2 inhibited 100% of the bacterial strain E. coli. The motivation of this study was to find a material with flexible and elastic properties and a hydrophobic character, with antibacterial properties that could be enhanced with silver nanoparticles, but hydrophobic properties that would be maintained. These properties can be used in different types of applications, mainly in tissue engineering and the medicinal industry, where water-repellent materials may play important roles. This synergy was achieved via the technique we proposed, and even when the Ag nanostructures were prepared, the high hydrophobicity of the system Ag-polytetrafluorethylene was maintained.
Black Tea Waste as Green Adsorbent for Nitrate Removal from Aqueous Solutions
Authors: Bondarev Andreea , Popovici Daniela Roxana , Călin Cătalina , Mihai Sonia , Sȋrbu Elena-Emilia , Doukeh Rami
Published: June 2023

The aim of the study was to prepare effective low-cost green adsorbents based on spent black tea leaves for the removal of nitrate ions from aqueous solutions. These adsorbents were obtained either by thermally treating spent tea to produce biochar (UBT-TT), or by employing the untreated tea waste (UBT) to obtain convenient bio-sorbents. The adsorbents were characterized before and after adsorption by Scanning Electron Microscopy (SEM), Energy Dispersed X-ray analysis (EDX), Infrared Spectroscopy (FTIR), and Thermal Gravimetric Analysis (TGA). The experimental conditions, such as pH, temperature, and nitrate ions concentration were studied to evaluate the interaction of nitrates with adsorbents and the potential of the adsorbents for the nitrate removal from synthetic solutions. The Langmuir, Freundlich and Temkin isotherms were applied to derive the adsorption parameters based on the obtained data. The maximum adsorption intakes for UBT and UBT-TT were 59.44 mg/g and 61.425 mg/g, respectively. The data obtained from this study were best fitted to the Freundlich adsorption isotherm applied to equilibrium (the values R2 = 0.9431 for UBT and R2 = 0.9414 for UBT-TT), this assuming the multi-layer adsorption onto a surface with a finite number of sites. The Freundlich isotherm model could explain the adsorption mechanism. These results indicated that UBT and UBT-TT could serve as novel biowaste and low-cost materials for the removal of nitrate ions from aqueous solutions.
Reliability Evaluation of Board-Level Flip-Chip Package under Coupled Mechanical Compression and Thermal Cycling Test Conditions
Authors: Shih Meng-Kai , Liu Yu-Hao , Lee Calvin , Hung C. P.
Published: June 2023

Flip Chip Ball Grid Array (FCBGA) packages, together with many other heterogeneous integration packages, are widely used in high I/O (Input/Output) density and high-performance computing applications. The thermal dissipation efficiency of such packages is often improved through the use of an external heat sink. However, the heat sink increases the solder joint inelastic strain energy density, and thus reduces the board-level thermal cycling test reliability. The present study constructs a three-dimensional (3D) numerical model to investigate the solder joint reliability of a lidless on-board FCBGA package with heat sink effects under thermal cycling testing, in accordance with JEDEC standard test condition G (a thermal range of −40 to 125 °C and a dwell/ramp time of 15/15 min). The validity of the numerical model is confirmed by comparing the predicted warpage of the FCBGA package with the experimental measurements obtained using a shadow moiré system. The effects of the heat sink and loading distance on the solder joint reliability performance are then examined. It is shown that the addition of the heat sink and a longer loading distance increase the solder ball creep strain energy density (CSED) and degrade the package reliability performance accordingly.
Effect of Replacing Fine Aggregate with Fly Ash on the Performance of Mortar
Authors: Zhang Dongsheng , Zhang Shuxiang , Yang Qiuning
Published: June 2023

Natural river sand resources are facing depletion, and large-scale mining pollutes the environment and harms humans. To utilize fly ash fully, this study used low-grade fly ash as a substitute for natural river sand in mortar. This has great potential to alleviate the shortage of natural river sand resources, reduce pollution, and improve the utilization of solid waste resources. Six types of green mortars were prepared by replacing different amounts of river sand (0, 20, 40, 60, 80, and 100%) with fly ash and other volumes. Their compressive strength, flexural strength, ultrasonic wave velocity, drying shrinkage, and high-temperature resistance were also investigated. Research has shown that fly ash can be used as a fine aggregate in the preparation of building mortar, thereby ensuring that green-building mortar has sufficient mechanical properties and better durability. The replacement rate for optimal strength and high-temperature performance was determined to be 80%.
Relationship between the Change in E/T Ratio and the Cooking Performance of Eucalyptus and Acacia Woods during Kraft Pulping Process
Authors: Yu Jiangdong , Xu Xuewen , Miao Chen , Li Penghui , Tong Guolin
Published: June 2023

Lignin structure is an important factor affecting the cooking part of the pulping process. In this study, the effect of lignin side chain spatial configuration on cooking performance was analyzed, and the structural characteristics of eucalyptus and acacia during cooking were compared and studied by combining ozonation, GC-MS, NBO, and 2D NMR (1H-13C HSQC). In addition, the changes in the lignin content of four different raw materials during the cooking process were studied via ball milling and UV spectrum analysis. The results showed that the content of lignin in the raw material decreased continuously during the cooking process. Only in the late cooking stage, when the lignin removal reached its limit, did the lignin content tend to be stable due to the polycondensation reaction of lignin. At the same time, the E/T ratio and S/G ratio of the reaction residual lignin also followed a similar rule. At the beginning of cooking, the values of E/T and S/G decreased rapidly and then gradually rose when they reached a low point. The different initial E/T and S/G values of different raw materials lead to the disunity of cooking efficiency and the different transformation rules of different raw materials in the cooking process. Therefore, the pulping efficiency of different raw materials can be improved using different technological means.
Synthesis and Assessment of Antimicrobial Composites of Ag Nanoparticles or AgNO3 and Egg Shell Membranes
Authors: Aina Samuel Tomi , Kyomuhimbo Hilda Dinah , Ramjee Shatish , Du Plessis Barend , Mjimba Vuyo , Maged Ali , Haneklaus Nils , Brink Hendrik Gideon
Published: June 2023

Engineering research has been expanded by the advent of material fusion, which has led to the development of composites that are more reliable and cost-effective. This investigation aims to utilise this concept to promote a circular economy by maximizing the adsorption of silver nanoparticles and silver nitrate onto recycled chicken eggshell membranes, resulting in optimized antimicrobial silver/eggshell membrane composites. The pH, time, concentration, and adsorption temperatures were optimized. It was confirmed that these composites were excellent candidates for use in antimicrobial applications. The silver nanoparticles were produced through chemical synthesis using sodium borohydride as a reducing agent and through adsorption/surface reduction of silver nitrate on eggshell membranes. The composites were thoroughly characterized by various techniques, including spectrophotometry, atomic absorption spectrometry, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy, as well as agar well diffusion and MTT assay. The results indicate that silver/eggshell membrane composites with excellent antimicrobial properties were produced using both silver nanoparticles and silver nitrate at a pH of 6, 25 °C, and after 48 h of agitation. These materials exhibited remarkable antimicrobial activity against Pseudomonas aeruginosa and Bacillus subtilis, resulting in 27.77% and 15.34% cell death, respectively.