bluuebunny/crossref_metadata_2025 huggingface dataset:
Towards Improving the Outcomes of Assisted Reproductive Technologies of Cattle and Sheep, with Particular Focus on Recipient Management
Authors: Daly Jamee , Smith Hayley , McGrice Hayley A. , Kind Karen L. , van Wettere William H.E.J.
Published: February 2020

The Australian agricultural industry contributes AUD 47 billion to the Australian economy, and Australia is the world’s largest exporter of sheep meat and the third largest for beef. Within Australia, sheep meat consumption continues to rise, with beef consumption being amongst the highest in the world; therefore, efficient strategies to increase herd/flock size are integral to the success of these industries. Reproductive management is crucial to increasing the efficiency of Australian breeding programs. The use of assisted reproductive technologies (ARTs) has the potential to increase efficiency significantly. The implementation of multiple ovulation and embryo transfer (MOET) and juvenile in vitro fertilization and embryo transfer (JIVET) in combination with genomic selection and natural mating and AI is the most efficient way to increase genetic gain, and thus increase reproductive efficiency within the Australian livestock industries. However, ARTs are costly, and high variation, particularly between embryo transfer recipients in their ability to maintain pregnancy, is a significant constraint to the widespread commercial adoption of ARTs. The use of a phenotypic marker for the selection of recipients, as well as the better management of recipient animals, may be an efficient and cost-effective means to increase the productivity of the Australian livestock industry.
Indicators of Horse Welfare: State-of-the-Art
Authors: Lesimple Clémence
Published: February 2020

Animal welfare is defined as a chronic state reflecting an individual’s subjective perception of its situation. Because it is possible to be in a good welfare state and nevertheless experience acute fear or pain, and conversely, short-term positive emotions can be experienced during impaired welfare states, welfare as a chronic state has to be clearly distinguished from temporary states related to emotions, pain or stress. The evaluation of non-verbal individuals’ welfare state, particularly in interspecific situations, is a real challenge that necessarily implies animal-based measures and requires multidisciplinary scientifically validated measures. In the last decade, studies investigating horses’ welfare flourished together with new measures that were not always scientifically tested before being used. At a time were legal decisions are made on animal welfare, it is crucial to rely on reliable welfare indicators in order to prevent false evaluation. The aim of this review is to identify the scientifically tested and reliable indicators of horses’ welfare (e.g., body lesions, apathy, aggressiveness, stereotypic behaviours) from signals of temporary states related to acute pain emotions or stress and from popular beliefs, in order to give the scientific community and the horse industry accurate evaluation tools.
The Effect of Kinesiotape on Flexion-Extension of the Thoracolumbar Back in Horses at Trot
Authors: Ericson Cajsa , Stenfeldt Pernilla , Hardeman Aagje , Jacobson Inger
Published: February 2020

Kinesiotape theoretically stimulates mechanoreceptive and proprioceptive sensory pathways that in turn may modulate the neuromuscular activity and locomotor function, so alteration of activation, locomotion and/or range of motion (ROM) can be achieved. The aim of this study was to determine whether kinesiotape applied to the abdominal muscles would affect the ROM in flexion-extension (sagittal plane) in the thoracolumbar back of horses at trot. The study design was a paired experimental study, with convenient sample. Each horse was randomly placed in the control or the intervention group and then the order reversed. Eight horses trotted at their own preferred speed in hand on a straight line, 2 × 30 m. Optical motion capture was used to collect kinematic data. Paired t-tests, normality tests and 1-Sample Wilcoxon test were used to assess the effects of the kinesiotape. No statistical significance (p < 0.05) for changes in flexion-extension of the thoracolumbar back in trot was shown in this group of horses. Some changes were shown indicating individual movement strategies in response to stimuli from the kinesiotape. More research in this popular and clinically used method is needed to fully understand the reacting mechanisms in horses.
Metagenomic Insights into Effects of Thiamine Supplementation on Carbohydrate-Active Enzymes’ Profile in Dairy Cows Fed High-Concentrate Diets
Authors: Zhao Yiguang , Xue Fuguang , Hua Dengke , Wang Yue , Pan Xiaohua , Nan Xuemei , Sun Fuyu , Jiang Linshu , Xiong Benhai
Published: February 2020

As the co-enzyme of pyruvate formate-lyase under ruminal anaerobic condition, thiamine plays a critical role in carbohydrate metabolism in dairy cows. The objective of this study was to investigate the impacts of thiamine supplementation on ruminal carbohydrate-active enzymes. Twelve Holstein dairy cows were randomly assigned into three dietary treatments: control diet (CON; 20% starch, dry matter (DM) basis), high-concentrate diet (HC; 33.2% starch, DM basis) and a high-concentrate diet supplemented with 180 mg thiamine/kg DM (HCT; 33.2% starch, DM basis). Dry matter intake and milk production were recorded for 21 days. Rumen fluid samples were collected, and ruminal pH and volatile fatty acids (VFAs) were measured. The metagenome sequencing technique was used to detect the genes in ruminal microorganisms and identify putative carbohydrate-active enzymes. The total abundances of carbohydrate-active enzymes and fiber-degrading enzymes were both reduced by HC with no effect on starch-degrading enzymes compared with CON. However, the fiber-degrading enzymes and starch-degrading enzymes were both increased after thiamine supplementation. These results indicated that 180 mg thiamine /kg DM might effectively improve rumen carbohydrate metabolism through increasing the abundance of ruminal carbohydrate-active enzymes and consequently balanced the rumen volatile fatty acids and rumen pH, providing a practical strategy in preventing subacute ruminal acidosis in cows offered HC.
Play in Elephants: Wellbeing, Welfare or Distraction?
Authors: Webber C. Elizabeth , Lee Phyllis C.
Published: February 2020

We explore elephant play behaviour since (a) play has been proposed to represent a potential welfare indicator; and (b) play has been associated with long-term survival in the wild. We categorised play into four types, and investigate both social (gentle, escalated-contact) and non-social (lone-locomotor, exploratory-object) play from observations made on wild (Asian N = 101; African N = 130) and captive (Asian N = 8; African N = 7) elephant calves ranging in age from birth to five years. Social play was the most frequent type of play among immature elephants, accounting for an average of 3%–9% of active time. Non-social play accounted for an additional 1%–11% of time. The most time spent in play was seen in captive Asian calves, particularly at the ages of 1–6 months, while wild African calves spent the least time in play overall, even though they had the greatest number and most diverse range of play partners available. We assessed calf energetics using time spent suckling, resting, moving and independent feeding. Time spent playing was unrelated to time spent suckling but negatively associated with time spent independently feeding. There were no associations with time spent moving or resting. Maternal energy via lactation was unrelated to play early in life, but energy acquired independently may constrain or enable play. Play, while a potential indicator of compromised welfare for many species when absent, can act as a highly stimulating activity for captive elephants in the absence of other forms of arousal.
Comparison of Single, Fixed-Time Artificial Insemination in Gilts Using Two Different Protocols to Synchronize Ovulation
Authors: Rodrigues Lima , Amezcua Rocio , Cassar Glen , O’Sullivan Terri L , Friendship Robert
Published: February 2020

In order to efficiently have a consistent supply of service-ready gilts available to incorporate into each batch of breeding sows, it is necessary to manipulate the timing of estrus and possibly the timing of ovulation of gilts. Estrus can be synchronized by the withdrawal of altrenogest after at least 14 days of treatment. It is possible that protocols developed to induce ovulation, and therefore allow fixed-time artificial insemination (FTAI), can improve the predictability of gilt breeding. This study investigated the effect of two FTAI protocols in gilts on reproductive performance and timing of farrowing and piglet weaning weight compared to gilts bred based on signs of estrus after cessation of altrenogest. Puberty was induced in gilts, followed by treatment with altrenogest. Following altrenogest withdrawal, 180 gilts were assigned to one of three treatment groups. Group 1 gilts (LUT, n = 62) were treated with 600 IU equine chorionic gonadotropin 24 h after altrenogest withdrawal and 5 mg porcine luteinizing hormone (pLH) 80 h later, followed by a single FTAI 36 h after pLH. Group 2 gilts (TRI, n= 61) received 2 mL of a gonadotropin-releasing hormone agonist, triptorelin acetate, intravaginally 6 d after altrenogest withdrawal and were bred by a single FTAI 24 h later. Group 3 gilts (CON, n = 57) were observed for estrus and bred twice by AI, 24 h apart. LUT and TRI gilts farrowed closer together (2.4 ± 1.6 and 2.9 ± 1.2 d(days), respectively) compared to CON gilts (4.5 ± 3.3 d). Piglets in LUT were 80 g (p < 0.001) heavier and piglets in TRI were 64 g (p < 0.05) heavier at weaning than CON piglets, when controlling for birth weight. Results indicate that FTAI might be useful as a means of minimizing the time from the first to the last gilt farrowing in a breeding batch of gilts. However, modifications of the protocols may be required to ensure optimum farrowing rates and litter size.
Association of SPOP Expression with the Immune Response to Salmonella Infection in Chickens
Authors: Wang Fei , Li Qinghe , Wang Qiao , Zheng Maiqing , Wen Jie , Zhao Guiping
Published: February 2020

Salmonellosis is a zoonosis that is not only harmful to the health of poultry but also poses a threat to human health. Although many measures have been put in place to reduce morbidity, they have not provided satisfactory results. Therefore, it is necessary to clarify the immune mechanisms involved in improving the resistance of chickens against Salmonella. BTB (Broad-complex Tramtrack and Bric-a-brac) Speckle-type POZ (poxvirus and zinc finger) protein (SPOP) regulates protein expression by promoting substrate ubiquitination and degradation. The correlation between SPOP expression and the immune response has not been fully described. Therefore, the aim of this study was to clarify this relationship. In vitro, we stimulated chicken macrophage cells (HD11) with lipopolysaccharide, then analyzed the correlation between SPOP and IL1β or IL8 expression using quantitative real-time polymerase chain reaction (qRT-PCR). In vivo, we infected 7-days-old chickens with Salmonella Typhimurium, then analyzed the association between SPOP expression and the immune response, including IL1β and IL8 expression, IgA production, and bacterial loads. We found that SPOP may participate in the regulation of the immune response in macrophage cells. SPOP expression was negatively correlated with IL-1β and IL-8 expression both in vivo and in vitro. SPOP expression was also negatively related to bacterial loads and immunoglobulin (Ig) A production. These results indicate that SPOP may have important functions in the response to Salmonella infection.
Relationship of Milk Odd- and Branched-Chain Fatty Acids with Urine Parameters and Ruminal Microbial Protein Synthesis in Dairy Cows Fed Different Proportions of Maize Silage and Red Clover Silage
Authors: Westreicher-Kristen Edwin , Castro-Montoya Joaquín , Hasler Mario , Susenbeth Andreas
Published: February 2020

The aim of this study was to evaluate the relationship of milk odd- and branched-chain fatty acids (OBCFA) with urinary purine derivates and estimated ruminal microbial crude protein (MCP) synthesis. Forty-four lactating Holstein cows were used in a 4 × 4 Latin square design with 21-day periods comprised of a 13-day adaptation phase to diet followed by an 8-day sampling phase. Differences in estimated MCP yield and milk OBCFA composition were found by feeding total mixed rations containing forage (maize silage, MS; red clover silage, RCS) and concentrates (0.75:0.25) with targeted proportions of RCS to MS of 0.15:0.60, 0.30:0.45, 0.45:0.30, and 0.60:0.15 on a dry matter basis. The MCP was estimated from the total urinary purine derivate (PD) excretion (MCPPD) and intakes of metabolizable energy (MCPME) or digestible organic matter (MCPdOM). The Pearson correlations of individual OBCFA with urinary parameters (uric acid, allantoin, PD and nitrogen) were generally weak (r = −0.37 to 0.55). Yields of individual OBCFA correlated positively with MCPME and MCPdOM (r = 0.21 to 0.55). The prediction of urinary PD concentration was moderate (R2 = 0.64) when including the proportion of iso-C17:0. The prediction of total PD excretion was low (R2 = 0.21) with yields of iso-C15:0, anteiso-C17:0, and iso-C16:0. The prediction of MCPPD was high (R2 = 0.99) when including the iso-C16:0 and cis-9 C17:1 concentrations, while those of MCPME and MCPdOM were low (R2 = 0.37 and 0.36, respectively) when including yields of iso-C15:0, cis-9 C17:1, and iso-C18:0. The correlations and regression analyses demonstrate that the estimated MCP synthesis and urinary PD excretion can be only moderately predicted by yields and concentrations of individual or total OBCFA in cow’s milk. However, milk OBCFA can still be seen as a promising, non-invasive method for predicting rumen function and microbial protein supply in dairy cows because MCP flow was not directly measured in this study but instead indirectly estimated probably comprising considerable deviations of the assumed values from the true ones.
Pain and Problem Behavior in Cats and Dogs
Authors: Mills Daniel S. , Demontigny-Bédard Isabelle , Gruen Margaret , Klinck Mary P. , McPeake Kevin J. , Barcelos Ana Maria , Hewison Lynn , Van Haevermaet Himara , Denenberg Sagi , Hauser Hagar , Koch Colleen , Ballantyne Kelly , Wilson Colleen , Mathkari Chirantana V , Pounder Julia , Garcia Elena , Darder Patrícia , Fatjó Jaume , Levine Emily
Published: February 2020

We argue that there is currently an under-reporting of the ways in which pain can be associated with problem behavior, which is seriously limiting the recognition of this welfare problem. A review of the caseloads of 100 recent dog cases of several authors indicates that a conservative estimate of around a third of referred cases involve some form of painful condition, and in some instances, the figure may be nearly 80%. The relationship is often complex but always logical. Musculoskeletal but also painful gastro-intestinal and dermatological conditions are commonly recognized as significant to the animal’s problem behavior. The potential importance of clinical abnormalities such as an unusual gait or unexplained behavioral signs should not be dismissed by clinicians in general practice, even when they are common within a given breed. In general, it is argued that clinicians should err on the side of caution when there is a suspicion that a patient could be in pain by carefully evaluating the patient’s response to trial analgesia, even if a specific physical lesion has not been identified.
Effects of Dietary Fat Sources during Late Gestation on Colostrum Quality and Mammary Gland Inflammation in Lipopolysaccharide-Challenged Sows
Authors: Zou Tiande , Wei Wenzhuo , Cao Shanchuan , Zhang Hongfu , Liu Jingbo
Published: February 2020

This study aimed to investigate the effects of maternal lipopolysaccharide (LPS) challenge and dietary fat sources on colostrum quality and inflammatory response in sows. Sixty Landrace × Yorkshire sows were randomly assigned to three dietary treatments supplemented with 3% soybean oil (SO), 3% coconut oil (CO) or 3% fish oil (FO), respectively, from Day 90 of gestation until parturition. On Day 112 of gestation, half the sows from each dietary treatment were challenged with LPS (10 μg/kg BW) or saline. The results showed that maternal LPS challenge decreased colostrum yield and dry matter content. A similar pattern of changes was observed for body weight gain and colostrum intake in piglets from LPS-challenged sows. Maternal LPS challenge increased the levels of tumor necrosis factor α (TNFα), interleukin 1β (IL1β) and IL6 in colostum, and the mRNA abundance of IL6, IL1β and TNFα and the phosphorylation level of p65 in mammary glands. However, the responses of these variables to LPS treatment were lower in sows fed a FO diet. In conclusion, maternal immune challenge reduced the growth performance of piglets by decreasing colostrum yield and intake by piglets, and dietary supplementation with FO in sows attenuates the LPS-induced inflammatory response in mammary glands.
Effects of Different Farrowing Environments on the Behavior of Sows and Piglets
Authors: Zhang Xiaojun , Li Congcong , Hao Yue , Gu Xianhong
Published: February 2020

We investigated the effect of different farrowing systems on the behavior of sows and piglets. In total, 22 hybrid sows (Yorkshire × Landrace) were randomly assigned into three farrowing systems, farrowing crate (FC), free farrowing pen with sloping walls (FFS), and free farrowing pen with sloping walls and nest materials (FFSN). The FFS and FFSN sows were more active, and exhibited less abnormal behaviors compared with the FC sows before and after parturition. FFS and FFSN piglets were more active compared with FC piglets. The increased activity of the FFS and FFSN sows might encourage the activity of their piglets, resulting in more proximity to sow behavior of their piglets. Providing nest materials improved the investigative behavior of sows and piglets. The total mortality of piglets in the free farrowing systems tended to be higher compared with the farrowing crate system. In conclusion, free farrowing system has beneficial effects on the behavior of sows and piglets but extra care in management needs to be taken to avoid piglet loss. Adding nest material in the farrowing pens is suggested to enrich the behavioral pattern of sows and piglets.
Etorphine-Azaperone Immobilisation for Translocation of Free-Ranging Masai Giraffes (Giraffa Camelopardalis Tippelskirchi): A Pilot Study
Authors: Vitali Francesca , Kariuki Edward K. , Mijele Domnic , Kaitho Titus , Faustini Massimo , Preziosi Richard , Gakuya Francis , Ravasio Giuliano
Published: February 2020

Etorphine-azaperone immobilisation was evaluated for translocation of Masai giraffes. Nine giraffes were darted with 0.012 ± 0.001 mg/kg etorphine and 0.07 ± 0.01 mg/kg azaperone. Once ataxic, giraffes were roped for recumbency and restrained manually. Naltrexone (3 mg/mg etorphine) was immediately given intravenously to reverse etorphine-related side effects. Protocol evaluation included physiological monitoring, blood-gas analyses, anaesthetic times, and quality scores (1 = excellent, 4 = poor). Sedation onset and recumbency were achieved in 2.6 ± 0.8 and 5.6 ± 1.4 min. Cardio-respiratory function (HR = 70 ± 16, RR = 32 ± 8, MAP = 132 ± 16) and temperature (37.8 ± 0.5) were stable. Arterial gas analysis showed hypoxaemia in some individuals (PaO2 = 67 ± 8 mmHg) and metabolic acidosis (pH = 7.23 ± 0.05, PaCO2 = 34 ± 4 mmHg, HCO3− = 12.9 ± 1.2 mmol/l). Minor startle response occurred, while higher induction-induced excitement correlated to longer inductions, worse restraint, and decreased HCO3−. After 19 ± 3.5 min of restraint, giraffes were allowed to stand and were loaded onto a chariot. Immobilisations were good and scored 2 (1–3). Inductions and recoveries were smooth and scored 1 (1–2). Translocations were uneventful and no complications occurred in 14-days boma follow-up.
Evaluation of the Associative Effects of Rice Straw with Timothy Hay and Corn Grain Using an In Vitro Ruminal Gas Production Technique
Authors: Sun Ling , Lee Mingyung , Jeon Seoyoung , Seo Seongwon
Published: February 2020

The objective of this study was to evaluate the associative effects of rice straw with timothy hay and corn grain. Using an automated gas production system, in vitro ruminal fermentation was studied for six substrates: 100% rice straw, 100% timothy hay, 100% corn grain, 50% rice straw and 50% timothy hay, 50% rice straw and 50% corn grain, and 50% rice straw, 25% timothy hay, and 25% corn grain. Incubation was performed in three batches with different rumen fluids to assess the in vitro ruminal gas production kinetics and rumen parameters (pH, NH3-N, volatile fatty acid (VFA), and true dry matter digestibility (TDMD)). The associated effects were tested by comparing the observed values of the composited feeds and the weighted means of individual feeds. There was a significant increase in NH3-N when rice straw was fermented with timothy hay, corn grain, or both (p < 0.05). TDMD increased when corn grain was co-fermented, and the total gas and VFA production increased when all three feeds were co-fermented. We conclude that the feed value of rice straw increases when fed to animals along with timothy hay and corn grain.
RETRACTED: Glutamine Metabolism and Its Role in Immunity, a Comprehensive Review
Authors: Shah Ali , Wang Zhisheng , Ma Jian
Published: February 2020

In the body of an animal, glutamine is a plentiful and very useful amino acid. Glutamine consumption in the body of animals in normal or disease conditions is the same or higher than the glucose. Many in vivo as well as in vitro experiments have been conducted to evaluate the importance of glutamine. Glutamine is a valuable nutrient for the proliferation of the lymphocytes. It also plays a crucial role in the production of cytokines, macrophages, phagocytic, and neutrophil to kill the bacteria. Most of the metabolic organs like the liver, gut, and skeletal muscles control the circulation and availability secretion of glutamine. In catabolic and hypercatabolic conditions, glutamine can turn out to be essential and plays a vital role in metabolism; however, availability may be compromised due to the impairment of homeostasis in the inter-tissue metabolism of amino acids. This is why the supplementation of glutamine is commonly used in clinical nutrition and is especially recommended to immune-suppressed persons. Despite this, in catabolic and hyper-catabolic conditions, it is challenging due to the amino acid concentration in plasma/bloodstream and glutamine should be provided via either the oral, enteral or parenteral route. However, the effect of glutamine as an immune-based supplement has been previously recognized as many research studies conducted in vivo and in-vitro evaluated the beneficial effects of glutamine. Hence, the present study delivers a combined review of glutamine metabolism in essential organs of the cell immune system. In this review, we have also reviewed the metabolism and action of glutamine and crucial problems due to glutamine supplementation in catabolic conditions.
Broad-Spectrum Cephalosporin-Resistant Klebsiella spp. Isolated from Diseased Horses in Austria
Authors: Loncaric Igor , Cabal Rosel Adriana , Szostak Michael P. , Licka Theresia , Allerberger Franz , Ruppitsch Werner , Spergser Joachim
Published: February 2020

The aim of the present study was to investigate the diversity of broad-spectrum cephalosporin-resistant Klebsiella spp. isolated from horses in Austria that originated from diseased horses. A total of seven non-repetitive cefotaxime-resistant Klebsiella sp. isolates were obtained during diagnostic activities from autumn 2012 to October 2019. Antimicrobial susceptibility testing was performed. The isolates were genotyped by whole-genome sequencing (WGS). Four out of seven Klebsiella isolates were identified as K. pneumoniae, two as K. michiganensis and one as K. oxytoca. All isolates displayed a multi-drug resistant phenotype. The detection of resistance genes reflected well the phenotypic resistance profiles of the respective isolates. All but one isolate displayed the extended-spectrum β-lactamases (ESBL) phenotype and carried CTX-M cefotaximases, whereas one isolate displayed an ESBL and AmpC phenotype and carried cephamycinase (CMY)-2 and sulfhydryl variable (SHV)-type b and Temoniera (TEM) β-lactamases. Among Klebsiella pneumoniae isolates, for different sequence types (ST) could be detected (ST147, ST307, ST1228, and a new ST4848). Besides resistance genes, a variety of virulence genes, including genes coding for yersiniabactin were detected. Considering the high proximity between horses and humans, our results undoubtedly identified a public health issue. This deserves to be also monitored in the years to come.
The Expression of ERK1/2 in Female Yak (Bos grunniens) Reproductive Organs
Authors: Fan Jiangfeng , Han Xiaohong , He Honghong , Luo Yuzhu , Yu Sijiu , Cui Yan , Xu Gengquan , Wang Libin , Pan Yangyang
Published: February 2020

The main reproductive organs undergo different histological appearances and physiological processes under different reproductive statuses. The variation of these organs depends on a delicate regulation of cell proliferation, differentiation, and apoptosis. Extracellular signal-regulated kinases1/2 (ERK1/2) are members of the mitogen-activated protein kinase (MAPK) super family. They have important roles in regulating various biological processes of different cells, tissues, and organ types. Activated ERK1/2 generally promotes cell survival, but under certain conditions, ERK1/2 also have the function of inducing apoptosis. It is widely believed that ERK1/2 play a significant role in regulating the reproductive processes of mammals. The goal of our research is to investigate the expression and distribution of ERK1/2 in the yak’s main reproductive organs during different stages. In the present study, samples of the ovary, oviduct, and uterus of 15 adult female yak were collected and used in the experiment. The ERK1/2 proteins, localization, and quantitative expression of their mRNA were investigated using immunohistochemistry (IHC), western blot (WB) and relative quantitative real-time polymerase chain reaction (RT-PCR). The results indicated that ERK1/2 proteins and their mRNA were highly expressed in the ovary of the luteal phase and gestation period, in the oviduct of the luteal phase, and in the uterus of the luteal phase and gestation period. Immunohistochemical analysis revealed a strong distribution of ERK1/2 proteins in follicular granulosa cells, granular luteal cells, villous epithelial cells of the oviduct, endometrial glandular epithelium, and luminal epithelium. These results demonstrated that the expression of ERK1 and ERK2 proteins and their mRNA in the yak’s ovary, oviduct, and uterus varies with the stage of the reproductive cycle. The variation character of ERK1 and ERK 2 expression in the yak’s main reproductive organs during different stages implies that they play an important role in regulating the reproductive function under different physiological statuses.
Genomic Analysis of Milk Protein Fractions in Brown Swiss Cattle
Authors: Macedo Mota Lucio Flavio , Pegolo Sara , Bisutti Vittoria , Bittante Giovanni , Cecchinato Alessio
Published: February 2020

Depending on whether milk protein fractions are evaluated qualitatively or quantitatively, different genetic outcomes may emerge. In this study, we compared the genetic parameters for the major milk protein fractions—caseins (αS1-, αS2-, β-, and к-CN), and whey proteins (β-lactoglobulin, β-LG; α-lactalbumin, α-LA)—estimated using the multi-trait genomic best linear unbiased prediction method and expressed variously as milk content (g/100g milk), percentage of milk nitrogen (%N) and daily yield per cow (g/d). The results showed that the genetic parameter estimates varied according to how the milk protein fractions were expressed. Heritability estimates for the caseins and whey protein fractions expressed as daily yields were lower than when they were expressed as proportions and contents, revealing important differences in genetic outcomes. The proportion and the content of β-CN were negatively correlated with the proportions and contents of αS1-CN, αS2-CN, and к-CN, while the daily yield of β–CN was negatively correlated with the daily yields of αS1-CN and αS2-CN. The Spearman’s rank correlations and the coincidence rates between the various predicted genomic breeding values (GEBV) for the milk protein fractions expressed in different ways indicated that these differences had a significant effect on the ranking of the animals. The results suggest that the way milk protein fractions are expressed has implications for breeding programs aimed at improving milk nutritional and technological characteristics.
Antibacterial Activity of Trypsin-Hydrolyzed Camel and Cow Whey and Their Fractions
Authors: Wang Ruixue , Han Zhihao , Ji Rimutu , Xiao Yuchen , Si Rendalai , Guo Fucheng , He Jing , Hai Le , Ming Liang , Yi Li
Published: February 2020

Antibacterial peptides were isolated and purified from whey proteins of camel milk (CaW) and cow milk (CoW) and their antimicrobial activities were studied. The whey proteins were hydrolyzed using trypsin, and the degree of hydrolysis was identified by gel electrophoresis. The whey hydrolysate (WH) was purified using ultrafiltration and Dextran gel chromatography to obtain small peptides with antibacterial activity. The effect of the antimicrobial peptides on the morphology of bacterial strains was investigated using transmission electron microscopy. Their amino acid composition and antimicrobial activities were then determined. Polypeptides CaWH-III (<3 kDa) and CoWH-III (<3 kDa) had the strongest antibacterial activity. Both Fr.A2 (CaWH-Ⅲ’s fraction 2) and Fr.B1 (CoWH-Ⅲ’s fraction 1) had antibacterial effects toward Escherichia coli and Staphylococcus aureus, with minimum antimicrobial mass concentrations of 65 mg/mL and 130 mg/mL for Fr.A2, and 130 mg/mL and 130 mg/mL for Fr.B1, respectively. The highly active antimicrobial peptides had high amounts of alkaline amino acids (28.13% in camel milk Fr.A2 and 25.07% in the cow milk Fr.B1) and hydrophobic amino acids. (51.29% in camel milk Fr.A2 and 57.69% in the cow milk Fr.B1). This results showed that hydrolysis of CaW and CoW using trypsin produced a variety of effective antimicrobial peptides against selected pathogens, and the antibacterial activity of camel milk whey was slightly higher than that of cow milk whey.
Comparative Transcriptomics Analysis of Testicular miRNA from Cryptorchid and Normal Horses
Authors: Han Haoyuan , Chen Qiuming , Gao Yuan , Li Jun , Li Wantao , Dang Ruihua , Lei Chuzhao
Published: February 2020

In the biological process of testicular spermatogenesis, the expression and interaction of many genes are regulated by microRNAs (miRNAs). However, comparisons of miRNA expression between descended testes (DTs) and undescended testes (UDTs) are rarely done in horses. In this study, we selected two UDTs (CKY2b and GU4b) from Chakouyi (CKY) and Guanzhong (GU) horses and eight DTs (GU1–3, CKY1, CKY3, CKY2a, GU4a, and GU5). Three groups were compared to evaluate expression patterns of testicular miRNA in stallion testes. Group 1 compared normal CKY horses and GU horses (CKY1 and CKY3 vs. GU1–3). Group 2 (CKY2a and GU4a (DTs) vs. CKY2b and GU4b (UDTs)) and group 3 (GU1–3, CKY1, CKY3 (DTs) vs. CKY2b and GU4b (UDTs)) compared the expression levels in unilateral retained testes to normal testes. The results show that 42 miRNAs (7 upregulated and 35 downregulated) had significantly different expression levels in both comparisons. The expression levels of eca-miR-545, eca-miR-9084, eca-miR-449a, eca-miR-9024, eca-miR-9121, eca-miR-8908e, eca-miR-136, eca-miR-329b, eca-miR-370, and eca-miR-181b were further confirmed by quantitative real-time PCR assay. The target genes of differentially expressed miRNAs in three comparisons were predicted, and the functions were annotated. The putative target genes of the 42 co-differentially expressed miRNAs were annotated to 15 functional terms, including metal ion binding, GTPase activator activity, zinc ion binding, intracellular, cytoplasm, and cancer pathways, and osteoclast differentiation. Our data indicate that the differentially expressed miRNAs in undescended testis suggests a potential role in male fertility and a relationship with cryptorchidism in horses. The discovery of miRNAs in stallion testes might contribute to a new direction in the search for biomarkers of stallion fertility.
Comparative Insight upon Chitosan Solution and Chitosan Nanoparticles Application on the Phenolic Content, Antioxidant and Antimicrobial Activities of Individual Grape Components of Sousão Variety
Authors: Silva Vanessa , Singh Rupesh Kumar , Gomes Nelson , Soares Bruno Gonçalves , Silva Adriana , Falco Virgílio , Capita Rosa , Alonso-Calleja Carlos , Pereira José Eduardo , Amaral Joana S. , Igrejas Gilberto , Poeta Patrícia
Published: February 2020

Chitosan, a natural polysaccharide, has been previously proposed as an elicitor in plants to prevent pathogen infections. The present study aimed to analyze the effect of chitosan solution and chitosan nanoparticles treatment applied on the grapevine variety Sousão with respect to the phenolic composition, antioxidant potential and antibacterial activity of its individual grape components. Grapevine plants of selected lines were sprayed with chitosan solution and chitosan nanoparticles, and ethanolic extracts of stems, seeds and skins were prepared from grapevines treated and not treated with chitosan. Total phenolic, anthocyanin and tannin contents were studied, and the identification of the individual phenolic compounds was performed by HPLC-DAD. The antimicrobial susceptibility method was performed using the Kirby-Bauer disc diffusion method against multidrug-resistant bacteria. Overall, there was small increase in the concentration of phenolic compounds, antioxidant and antimicrobial activities in grape components treated with chitosan solution. Seed extracts showed the highest antioxidant and antimicrobial activities. The studied individual components obtained from chitosan-treated grapevines could represent an added value due to the increased antioxidant and antibacterial potentials. The phenolic compounds found in components may be used in food and pharmaceutical industries as natural food preservers and antibiotic adjuvants.
Laser Intensity Noise Suppression for Preparing Audio-Frequency Squeezed Vacuum State of Light
Authors: Bai Lele , Wen Xin , Yang Yulin , He Jun , Wang Junmin
Published: February 2020

Laser intensity noise suppression has essential effects on preparation and characterization of the audio-frequency squeezed vacuum state of light based on a sub-threshold optical parametric oscillator (OPO). We have implemented two feedback loops by using relevant acousto-optical modulators (AOM) to stabilize the intensity of 795-nm near infrared (NIR) fundamental laser and 397.5-nm ultraviolet (UV) laser generated by cavity-enhanced frequency doubling. Typical peak-to-peak laser intensity fluctuation with a bandwidth of ~10 kHz in a half hour has been improved from ±7.45% to ±0.06% for 795-nm NIR laser beam, and from ±9.04% to ±0.05% for 397.5-nm UV laser beam, respectively. The squeezing level of the squeezed vacuum state at 795 nm prepared by the sub-threshold OPO with a PPKTP crystal has been improved from −3.3 to −4.0 dB around 3~9 kHz of analysis frequency range.
Comparison of Selected Parameters of a Planetary Gearbox with Involute and Convex–Concave Teeth Flank Profiles
Authors: Brumercik Frantisek , Lukac Michal , Caban Jacek , Krzysiak Zbigniew , Glowacz Adam
Published: February 2020

This article presents a model of the geometry of teeth profiles based on the path of contact definition. The basic principles of the involute and convex–concave teeth profile generation are described. Due to the more difficult manufacturing of the convex–concave gear profile in comparison to the involute one, an application example was defined that suppressed this disadvantage, namely a planetary gearbox with plastic-injection-molded gears commonly used in vehicle back-view mirror positioners. The contact pressures and the slide ratios of the sun, planet, and ring gears with both teeth profile variants were observed and the differences between the calculated parameters are discussed.
Method for Quantitative Broadband Diffuse Optical Spectroscopy of Tumor-Like Inclusions
Authors: Vasudevan Sandhya , Forghani Farnoush , Campbell Chris , Bedford Savannah , O’Sullivan Thomas D.
Published: February 2020

A hybrid reflectance-based diffuse optical imaging (DOI) technique combining discrete wavelength frequency-domain (FD) near-infrared spectroscopy (NIRS) with broadband continuous wave NIRS measurements was developed to quantify the broadband optical properties of deep tumor-like inclusions. This method was developed to more accurately measure the broadband optical properties of human tumors using a compact handheld imaging probe and without requiring a priori spectral constraints. We simulated the reconstruction of absorption and scattering spectra (650–1000 nm) of human breast tumors in a homogeneous background at depths of 0 to 10 mm. The hybrid DOI technique demonstrated enhanced performance in reconstruction of optical absorption with a mean accuracy over all 71 wavelengths of 8.39% versus 32.26% for a 10 mm deep tumor with the topographic DOI method. The new hybrid technique was also tested and validated on two heterogeneous tissue-simulating phantoms with inclusion depths of 2, 7, and 9 mm. The mean optical absorption accuracy over all wavelengths was similarly improved up to 5x for the hybrid DOI method versus topographic DOI for the deepest inclusions.
Dual Thermal-/Electrical-Responsive Luminescent ‘Smart’ Window
Authors: Timmermans Gilles H. , Douma Robin F. , Lin Jianbin , Debije Michael G.
Published: February 2020

As buildings are a large energy user, it is important to not only reduce their consumption, but also have them generate their own electricity. Here, we describe a smart window that could reduce electricity consumption, normally used for air conditioning and lighting, by absorbing excess solar radiation with dichroic fluorescent dye molecules aligned in a switchable liquid crystal host and guiding the re-emitted light energy to the edges of the device, where it can be used to generate electricity via attached photovoltaic cells. The liquid crystals are responsive both to temperature changes and applied electrical fields. At higher temperatures, transmission decreases due to increased disorder in the liquid crystals, while the application of an electrical field increases transmission by effectively realigning the dyes for minimal absorption. Using alternative configurations, a window with a transparent rest state was also produced, in which transmission can be decreased by applying an electrical field; the thermal response remains identical.
Data Driven Water Surface Elevation Forecasting Model with Hybrid Activation Function—A Case Study for Hangang River, South Korea
Authors: Yoo Hyung Ju , Kim Dong Hyun , Kwon Hyun-Han , Lee Seung Oh
Published: February 2020

To date, physical, numerical or data-driven models have been used to forecast water surface elevation in rivers for specific times or locations in the literature. Recently, the trend of forecasting water surface elevation changed from physical and numerical models to data-driven models with the help of the development of big data processing technology and fast simulating time of data-driven models. In this study, a data-driven model with Long Short-Term Memory (LSTM) was developed using TensorFlow, one of the famous deep learning frameworks and forecasting of water surface elevation affected tidal river was performed in Hangang River, Korea. From many types of field measurements, the hourly hydrological data, precipitation, outlet discharge of dam upstream and tidal levels were selected as the input dataset through a t-test and a p-value. In particular, the hybrid activation function was proposed to alleviate the vanishing gradient and dying neuron problems generally issued in the application of the activation function. The model showed that the root mean square error (RMSE) and peak error (PE) decreased by 0.22–0.25 m and 0.11–0.21 m, respectively, and the Nash-Sutcliffe efficiency (NSE) increased up to 79.3%–97.0% compared with the single activation functions. For w 1 = 0.6 and w 2 = 0.4 in the hybrid activation function, the improvement of accuracy and the enhancement of the application range of the leading time interval were obtained through a sensitivity analysis. Moreover, the hybrid activation function showed a good performance. The forecasting results provided by this model can be used as reference data for the establishment of the emergency action plan (EAP).
Experimental Study on the Flexural Behavior of Steel-Textile-Reinforced Concrete: Various Textile Reinforcement Details
Authors: You Jungbhin , Park Jongho , Park Sun-Kyu , Hong Sungnam
Published: February 2020

In this study, one reinforced concrete specimen and six textile reinforced concrete (TRC) specimens were produced to analyze the flexural behavior of steel-textile-reinforced concrete. The TRC specimen was manufactured using a total of four variables: textile reinforcement amount, textile reinforcement hook, textile mesh type, textile lay out form. Flexural performance increases with textile reinforcement amount, textile reinforcement hook type and textile reinforcement mesh type. The flexural performance was improved when physical hooks were used. Furthermore, textile reinforcement was verified as being effective at controlling the deflection.
Microwave Healing Performance of Asphalt Mixture Containing Electric Arc Furnace (EAF) Slag and Graphene Nanoplatelets (GNPs)
Authors: Gulisano Federico , Crucho João , Gallego Juan , Picado-Santos Luis
Published: February 2020

Pavement preventive maintenance is an important tool for extending the service life of the road pavements. Microwave heating seems to be a promising technology for this application, as bituminous materials have the potential to self-repair above a certain temperature. As ordinary asphalt mixture has low microwave absorbing properties, some additives should be used to improve the heating efficiency. In this paper, the effect of adding Electric Arc Furnace (EAF) slag and Graphene Nanoplatelets (GNPs) on the microwave heating and healing efficiency of asphalt mixtures was evaluated. Microwave heating efficiency was assessed by heating the specimens using several heating times. In addition, the electrical resistivity of the mixtures was measured to understand its possible relationship with the microwave heating process. Furthermore, the healing rates of the asphalt mixtures were assessed by repeated Indirect Tensile Strength (ITS) tests. The results obtained indicate that the additions of graphene and EAF slag can allow important savings, up to 50%, on the energy required to perform a good healing process.
Phase Space Reconstruction from a Biological Time Series: A Photoplethysmographic Signal Case Study
Authors: de Pedro-Carracedo Javier , Fuentes-Jimenez David , Ugena Ana María , Gonzalez-Marcos Ana Pilar
Published: February 2020

In the analysis of biological time series, the state space is comprised of a framework for the study of systems with presumably deterministic and stationary properties. However, a physiological experiment typically captures an observable that characterizes the temporal response of the physiological system under study; the dynamic variables that make up the state of the system at any time are not available. Only from the acquired observations should state vectors be reconstructed to emulate the different states of the underlying system. This is what is known as the reconstruction of the state space, called the phase space in real-world signals, in many cases satisfactorily resolved using the method of delays. Each state vector consists of m components, extracted from successive observations delayed a time τ . The morphology of the geometric structure described by the state vectors, as well as their properties depends on the chosen parameters τ and m. The real dynamics of the system under study is subject to the correct determination of the parameters τ and m. Only in this way can be deduced features have true physical meaning, revealing aspects that reliably identify the dynamic complexity of the physiological system. The biological signal presented in this work, as a case study, is the photoplethysmographic (PPG) signal. We find that m is five for all the subjects analyzed and that τ depends on the time interval in which it is evaluated. The Hénon map and the Lorenz flow are used to facilitate a more intuitive understanding of the applied techniques.
Reliability Analysis and Imprecise Component Importance Measure of Redundant Systems of OWTs Based on Component Swapping
Authors: Li Yao , Zhu Caichao , Wang Zi
Published: February 2020

Due to the high cost of failures of wind turbines, redundancy designs are commonly applied in wind turbines for improving the reliability and availability of systems. For this reason, replacing failed components with other working components of the same type in redundant systems is becoming an attractive option of maintenance strategies towards more resilient systems. To quantitatively evaluate system’s reliability, this paper focuses on the reliability analysis of redundant systems of offshore wind turbines based on swapping existing components. The survival signature-based component swapping method is introduced to describe the new structure-function of the system upon swapping. Furthermore, the reliability model of redundant systems is established using the fault tree and survival signature. Following this, the influences of component swapping on component reliability importance measure (marginal reliability importance and joint reliability importance) without and with considerations of the imprecision of failure rates are explored. Finally, a 5MW offshore wind turbine is presented to show the applicability of the proposed approach for redundant systems, and the results show that the proposed approach can obtain realistic reliability assessment of redundant systems and considering component swapping can significantly improve system reliability.
Unrepeatered 240-km 64-QAM Transmission Using Distributed Raman Amplification over SMF Fiber
Authors: Rosa P. , Rizzelli G. , Pang X. , Ozolins O. , Udalcovs A. , Tan M. , Jaworski M. , Marciniak M. , Sergeyev S. , Schatz R. , Jacobsen G. , Popov S. , Ania-Castañón J. D.
Published: February 2020

We present a theoretical and experimental investigation of unrepeatered transmission over standard single-mode fiber (SMF-28) using several schemes of distributed Raman amplification, including first, second, and dual order. In order to further extend the transmission distance, we utilize advanced bidirectional higher-order ultra-long Raman fiber laser-based amplification, where we use fiber Bragg gratings (FBGs) to reflect Stokes-shifted light from the secondary pumps. Our work demonstrates the possibility of transmission up to 240-km span length with a total span loss of 52.7 dB. Here, we use a 28-Gbaud signal using a 64-quadrature amplitude modulation (QAM) modulation format. Our results highlight the contribution of nonlinear compensation using digital back propagation in a digital signal processor (DSP) code at the receiver.