bluuebunny/crossref_metadata_2025 huggingface dataset:
Intratumor microbiome derived glycolysis-lactate signatures depicts immune heterogeneity in lung adenocarcinoma by integration of microbiomic, transcriptomic, proteomic and single-cell data
Authors: Deng Xiaheng , Chen Xiru , Luo Yu , Que Jun , Chen Liang
Published: August 2023

Introduction

Microbiome plays roles in lung adenocarcinoma (LUAD) development and anti-tumor treatment efficacy. Aberrant glycolysis in tumor might promote lactate production that alter tumor microenvironment, affecting microbiome, cancer cells and immune cells. We aimed to construct intratumor microbiome score to predict prognosis of LUAD patients and thoroughly investigate glycolysis and lactate signature’s association with LUAD immune cell infiltration.

Methods

The Cancer Genome Atlas-LUAD (TCGA-LUAD) microbiome data was downloaded from cBioPortal and analyzed to examine its association with overall survival to create a prognostic scoring model. Gene Set Enrichment Analysis (GSEA) was used to find each group’s major mechanisms involved. Our study then investigated the glycolysis and lactate pattern in LUAD patients based on 19 genes, which were correlated with the tumor microenvironment (TME) phenotypes and immunotherapy outcomes. We developed a glycolysis-lactate risk score and signature to accurately predict TME phenotypes, prognosis, and response to immunotherapy.

Results

Using the univariate Cox regression analysis, the abundance of 38 genera were identified with prognostic values and a lung-resident microbial score (LMS) was then developed from the TCGA-LUAD-microbiome dataset. Glycolysis hallmark pathway was significantly enriched in high-LMS group and three distinct glycolysis-lactate patterns were generated. Patients in Cluster1 exhibited unfavorable outcomes and might be insensitive to immunotherapy. Glycolysis-lactate score was constructed for predicting prognosis with high accuracy and validated in external cohorts. Gene signature was developed and this signature was elevated in epithelial cells especially in tumor mass on single-cell level. Finally, we found that the glycolysis-lactate signature levels were consistent with the malignancy of histological subtypes.

Discussion

Our study demonstrated that an 18-microbe prognostic score and a 19-gene glycolysis-lactate signature for predicting prognosis of LUAD patients. Our LMS, glycolysis-lactate score and glycolysis-lactate signature have potential roles in precision therapy of LUAD patients.
High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae
Authors: Stazzoni Samuele , Troisi Marco , Abbiento Valentina , Sala Claudia , Andreano Emanuele , Rappuoli Rino
Published: August 2023

Neisseria gonorrhoeae (gonococcus) is an obligate human pathogen and the etiological agent of the sexually transmitted disease gonorrhea. The rapid rise in gonococcal resistance to all currently available antimicrobials has become a significant public health burden and the need to develop novel therapeutic and prophylactic tools is now a global priority. While high-throughput screening methods allowed rapid discovery of extremely potent monoclonal antibodies (mAbs) against viral pathogens, the field of bacteriology suffers from the lack of assays that allow efficient screening of large panels of samples. To address this point, we developed luminescence-based (L-ABA) and resazurin-based (R-ABA) antibody bactericidal assays that measure N. gonorrhoeae metabolic activity as a proxy of bacterial viability. Both L-ABA and R-ABA are applicable on the large scale for the rapid identification of bactericidal antibodies and were validated by conventional methods. Implementation of these approaches will be instrumental to the development of new medications and vaccines against N. gonorrhoeae and other bacterial pathogens to support the fight against antimicrobial resistance.
First identification of canine adenovirus 1 in mink and bioinformatics analysis of its 100 K protein
Authors: Hou Jinyu , Xu Jinfeng , Wang Ben , Zhang Hongling , Yin Baishuang , Li Goujiang , Lei Fashou , Cai Xiaoming , Zhu Yanzhu , Wang Longtao
Published: August 2023

Introduction

Animal trade favors the spreading of emerging canine adenovirus 1 (CAdV-1) in mink. Because the 100K protein is not exposed to the viral surface at any stage, it can be used to differentiate the vaccine from wild virus infection. However, no related research has been conducted. This study aimed to find evidence of CAdV-1 in mink and predict the character of the 100K protein in the current circulating CAdV-1 strain of mink.

Method

In this experiment, the identification of CAdV-1, the phylogenetic tree, homology, and bioinformatics analysis of 100K were conducted.

Results

The results showed that the CAdV-1 was identified in the mink and that its Fiber was located in a separate branch. It was closely related to strains isolated from Norwegian Arctic fox and Red fox. 100K was located in a separate branch, which had the closest genetic relationship with skunks, porcupines, raccoons, and hedgehogs and a far genetic relationship with the strains in dogs. 100K protein is an unstable and hydrophobic protein. It had evidence of selective pressure and recombination, 1 glycosylation site, 48 phosphorylation sites, 60 dominant B cell epitopes, and 9 peptides of MHC-I and MHC-II. Its subcellular localization was mainly in the endoplasmic reticulum and mitochondria. The binding sites of 100K proteins were DBP proteins and 33K proteins.

Discussion

The stains in the mink were different from fox. The exploration of its genomic characteristics will provide us with a deeper understanding of the prevention of canine adenovirus.
Secular trends and features of thalamic hemorrhages compared with other hypertensive intracerebral hemorrhages: an 18-year single-center retrospective assessment
Authors: Katano Hiroyuki , Nishikawa Yusuke , Uchida Mitsuru , Yamanaka Tomoyasu , Hayashi Yuki , Yamada Shigeki , Tanikawa Motoki , Yamada Kazuo , Mase Mitsuhito
Published: August 2023

Introduction

Trends regarding the locations of hypertensive cerebral hemorrhages are unclear. To clarify hypertensive hemorrhage trends, we investigated intracerebral hemorrhages (ICHs) over an 18-year period, focusing on thalamic hemorrhages compared with other sites of hemorrhages.

Methods

We reviewed the cases of patients hospitalized for hypertensive ICH in 2004–2021 at our hospital; 1,320 eligible patients were registered with a primary ICH/intraventricular hemorrhage. After exclusion criteria were applied, we retrospectively analyzed 1,026 hypertensive ICH cases.

Results

The proportions of thalamic and subcortical hemorrhages increased over the 18-year period, whereas putaminal hemorrhage decreased. Multivariate logistic regression analyses revealed that for thalamic hemorrhage, ≥200 mmHg systolic blood pressure (p = 0.031), bleeding <15 mL (p = 0.001), and higher modified Rankin scale (mRS) score ≥ 4 at discharge (p = 0.006) were significant variables in the late period (2013–2021) versus the early period (2004–2012), whereas for putaminal hemorrhage, significant factors in the late period were triglyceride <150 mg/dL (p = 0.006) and mRS score ≥ 4 at discharge (p = 0.002). Among the features of the thalamic hemorrhages in the late period revealed by our group comparison with the putaminal and subcortical hemorrhages, the total and subcortical microbleeds were more notable in the thalamic hemorrhages than in the other two types of hemorrhage, whereas cerebellar microbleeds were more prominent when compared only with subcortical hemorrhages.

Discussion

Our findings revealed an increasing trend for thalamic hypertensive hemorrhage and a decreasing trend for putaminal hemorrhage. The thalamic hemorrhage increase was observed in both young and older patients, regardless of gender. The main features of thalamic hemorrhage in the late period versus the early period were decrease in larger hemorrhage (≥15 mL) and an increase in cases with higher systolic blood pressure (at least partially involved a small number of untreated hypertensive patients who developed major bleeding). The total and subcortical microbleeds were more notable in the thalamic hemorrhages of the late period than in the putaminal and subcortical hemorrhages. These results may contribute to a better understanding of the recent trends of hypertensive ICHs and may help guide their appropriate treatments for this condition.
Healthcare-associated infections and antimicrobial resistance in severe acquired brain injury: a retrospective multicenter study
Authors: Castellani Giovanna Barbara , Maietti Elisa , Leonardi Gloria , Bertoletti Erik , Trapani Filippo , Battistini Alberto , Tedeschi Sara , Piperno Roberto , Viale Pierluigi
Published: August 2023

Background

Recent studies underscore that healthcare-associated infections (HAIs) and multidrug-resistant (MDR) HAIs affect rehabilitation outcomes and hospital length of stay (LOS) for severe acquired brain injury (sABI).

Objective

This study aimed to estimate HAI incidence in different sABI rehabilitation settings and determine risk factors and HAI impact on neuromotor and cognitive recovery.

Methods

We conducted a retrospective multicenter study in two semi-intensive units (SICUs), two high-specialty post-acute units (PAUs), and one long-term care (LTC) rehabilitation facility. Data extraction was performed by experienced clinicians, using a structured Excel file and they agreed upon criteria for case definitions of healthcare. The main outcome measures were the HAI and MDR HAI incidence and the LOS, the functional recovery was measured using the Level of Cognitive Functioning and Disability Rating Scale.

Results

There were 134 sABI participants. The calculation of the probability level was adjusted for three pairwise comparisons among settings (0.05/3 = 0.017). The HAI and MDR HAI incidences were significantly higher in SICU (3.7 and 1.3 per 100 person-days) than in other settings (LTC: 1.9, p = 0.034 and 0.5, p = 0.026; PAU: 1.2, p < 0.001 and 0.3, p < 0.001). HAI and MDR HAI risk variables included older age, an increased number of devices, and carbapenemase-producing Enterobacteriaceae (CPE) colonization, while a high prealbumin plasma value seemed to have a protective effect.

Conclusion

HAIs are related to longer LOS, and colonization is associated with poor prognosis and poor functional outcomes with reduced ability to achieve the cognitive capacity of self-care, employability, and independent living. The need to ensure the protection of non-colonized patients, especially those with severe disabilities on admission, is highlighted.
Common microRNA regulated pathways in Alzheimer’s and Parkinson’s disease
Authors: Awuson-David Betina , Williams Adrian C. , Wright Benjamin , Hill Lisa J. , Di Pietro Valentina
Published: August 2023

MicroRNAs (miRNAs) are small non-coding RNAs involved in gene regulation. Recently, miRNA dysregulation has been found in neurodegenerative diseases such as Alzheimer’s disease (AD) and Parkinson’s disease (PD). The diagnosis of Alzheimer’s and Parkinson’s is currently challenging, mainly occurring when pathology is already present, and although treatments are available for both diseases, the role of treatment is primarily to prevent or delay the progress of the diseases instead of fully overcoming the diseases. Therefore, the challenge in the near future will be to determine effective drugs to tackle the dysregulated biological pathways in neurodegenerative diseases. In the present study, we describe the dysregulation of miRNAs in blood of Alzheimer’s and Parkinson’s patients with the aim to identify common mechanisms between the 2 pathologies and potentially to identify common therapeutic targets which can stop or delay the progression of two most frequent neuropathologies. Two independent systematic reviews, bioinformatic analysis, and experiment validation were performed to identify whether AD and PD share common pathways. A total of 15 common miRNAs were found in the literature and 13 common KEGG pathways. Among the common miRNAs, two were selected for validation in a small cohort of AD and PD patients. Let-7f-5p and miR-29b-3p showed to be good predictors in blood of PD patients.
Evaluating the expression of heat shock protein 27 and topoisomerase II α in a retrospective cohort of patients diagnosed with locally advanced breast cancer and treated with neoadjuvant anthracycline-based chemotherapies
Authors: Zhuang Yixuan , Zhang Fan , Xu Yue , He Lifang , Huang Wenhe , Hong Chaoqun , Cui Yukun
Published: August 2023

Background

Neoadjuvant anthracycline-based chemotherapy (NAC) is a major regimen for the treatment of local advanced breast cancer (LABC), while resistance to NAC remains a paramount clinical obstacle. To investigate the role of heat shock protein 27 (Hsp27) and/or topoisomerase IIα (TopoIIα) in LABC patients treated with NAC, we performed this retrospective study.

Methods

Associations of Hsp27 transcripts with clinic-pathological characteristics, survival and drug response were investigated in public databases. Hsp27-related genes were identified, followed by functional enrichment analyses. Besides, two protein-protein interaction networks were built. Then, tumors from 103 patients who were diagnosed with LABC and received NAC were collected, and Hsp27 and TopoIIα were examined by Immunohistochemistry (IHC). Chi-square or Fisher’s exact tests were performed, as well as survival analyses.

Results

Either at the transcriptional level in public databases or at the protein level tested by IHC, a high level of Hsp27 was associated with aggressive tumor characteristics such as lymph node invasion and chemotherapy resistance. Hsp27-related genes mostly involved in the metabolic pathway and the gamete generation biological process. An elevated Hsp27 indicated a poor prognosis in patients with breast cancer (log-rank test P = 0.002 and 0.004 for disease-free survival [DFS] and overall survival [OS], respectively), while it might not be an independent predictor. Of note, tumors with high TopoIIα expression (TopoIIα+) was less likely to express Hsp27 (Hsp27+), in contrast to those with TopoIIα negativity (31.1% vs. 86.2%, P<0.001), and survival analyses revealed that patients with Hsp27+ and TopoIIα- tumors had a significantly lower DFS and OS (log-rank test P < 0.001 and 0.001, respectively), in contrast to the other three groups.

Conclusions

Hsp27 was associated with aggressive breast cancers and more predictable for the prognosis of LABC patients treated with NAC when concomitantly considering TopoIIα expression.
Case Report: Durable therapy response to Osimertinib in rare EGFR Exon 18 mutated NSCLC
Authors: Cekay Michael , Arndt Philipp F. , Dumitrascu Rio , Savai Rajkumar , Braeuninger Andreas , Gattenloehner Stefan , Steiner Dagmar , Roller Fritz , Tello Khodr , Hattar Katja , Seeger Werner , Sibelius Ulf , Grimminger Friedrich , Eul Bastian
Published: August 2023

Up to 20% of all non-small cell lung cancer patients harbor tumor specific driver mutations that are effectively treated with tyrosine kinase inhibitors. However, for the rare EGFR deletion-insertion mutation of exon 18, there is very little evidence regarding the effectiveness of tyrosine kinase inhibitors. A particular challenge for clinicians in applying tyrosine kinase inhibitors is not only diagnosing a mutation but also interpreting rare mutations with unclear therapeutic significance. Thus, we present the case of a 65-year-old Caucasian male lung adenocarcinoma patient with an EGFR Exon 18 p.Glu709_Thr710delinsAsp mutation of uncertain therapeutic relevance. This patient initially received two cycles of standard platinum-based chemotherapy without any therapeutic response. After administration of Osimertinib as second line therapy, the patient showed a lasting partial remission for 12 months. Therapy related toxicities were limited to mild thrombocytopenia, which ceased after dose reduction of Osimertinib. To our knowledge, this is the first report of effective treatment of this particular mutation with Osimertinib. Hence, we would like to discuss Osimertinib as a viable treatment option in EGFR Exon 18 p.Glu709_Thr710delinsAsp mutated lung adenocarcinoma.
Prevalence of PIK3CA mutations in Taiwanese patients with breast cancer: a retrospective next-generation sequencing database analysis
Authors: Chao Ta-Chung , Tsai Yi-Fang , Liu Chun-Yu , Lien Pei-Ju , Lin Yen-Shu , Feng Chin-Jung , Chen Yen-Jen , Lai Jiun-I. , Hsu Chih-Yi , Lynn Jiun Jen , Huang Chi-Cheng , Tseng Ling-Ming
Published: August 2023

Background

Breast cancer is the most common cancer type that affects women. In hormone receptor–positive (HR+), human epidermal growth factor receptor 2−negative (HER2–) advanced breast cancer (ABC), phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) is the most frequently mutated gene associated with poor prognosis. This study evaluated the frequency of PIK3CA mutations in the Taiwanese breast cancer population.

Methodology

This is a retrospective study; patient data were collected for 2 years from a next-generation sequencing database linked to electronic health records (EHRs). The primary endpoint was the regional prevalence of PIK3CA mutation. The secondary endpoints were to decipher the mutation types across breast cancer subtype, menopausal status, and time to treatment failure after everolimus (an mTOR inhibitor) or cyclin-dependent kinase 4/6 (CDK4/6) inhibitor treatment.

Results

PIK3CA mutations were identified in 278 of 728 patients (38%). PIK3CA mutations were reported in 43% of patients with HR−/HER2+ subtype and 42% of patients with HR+/HER2– postmenopausal status. A lower prevalence of PIK3CA mutations was observed in triple-negative (27%) and HR+/HER2– premenopausal patients (29%). The most common mutation was at exon 20 (H1047R mutation, 41.6%), followed by exon 9 (E545K mutation, 18.9% and E542K mutation, 10.3%). Among patients treated with CDK4/6 inhibitors, the median time to treatment failure was 12 months (95% CI: 7-21 months) in the PIK3CA mutation cohort and 16 months (95% CI: 11-23 months) in the PIK3CA wild-type cohort, whereas patients receiving an mTOR inhibitor reported a median time to treatment failure of 20.5 months (95% CI: 8-33 months) in the PIK3CA mutation cohort and 6 months (95% CI: 2-9 months) in the PIK3CA wild-type cohort.

Conclusion

A high frequency of PIK3CA mutations was detected in Taiwanese patients with breast cancer, which was consistent with previous studies. Early detection of PIK3CA mutations might influence therapeutic decisions, leading to better treatment outcomes.
Phase 3 CLEAR study in patients with advanced renal cell carcinoma: outcomes in subgroups for the lenvatinib-plus-pembrolizumab and sunitinib arms
Authors: Grünwald Viktor , Powles Thomas , Eto Masatoshi , Kopyltsov Evgeny , Rha Sun Young , Porta Camillo , Motzer Robert , Hutson Thomas E. , Méndez-Vidal María José , Hong Sung-Hoo , Winquist Eric , Goh Jeffrey C. , Maroto Pablo , Buchler Tomas , Takagi Toshio , Burgents Joseph E. , Perini Rodolfo , He Cixin , Okpara Chinyere E. , McKenzie Jodi , Choueiri Toni K.
Published: August 2023

Introduction

The phase 3 CLEAR study demonstrated that lenvatinib plus pembrolizumab significantly improved efficacy versus sunitinib as first-line treatment for patients with advanced renal cell carcinoma (RCC). Prognostic features including presence and/or site of baseline metastases, prior nephrectomy, and sarcomatoid features have been associated with disease and treatment success. This subsequent analysis explores outcomes in patients with or without specific prognostic features.

Methods

In CLEAR, patients with clear cell RCC were randomly assigned (1:1:1) to receive either lenvatinib (20 mg/day) plus pembrolizumab (200 mg every 3 weeks), lenvatinib (18 mg/day) plus everolimus (5 mg/day), or sunitinib alone (50 mg/day, 4 weeks on, 2 weeks off). In this report, progression-free survival (PFS), overall survival (OS), and objective response rate (ORR) were all assessed in the lenvatinib-plus-pembrolizumab and the sunitinib arms, based on baseline features: lung metastases, bone metastases, liver metastases, prior nephrectomy, and sarcomatoid histology.

Results

In all the assessed subgroups, median PFS was longer with lenvatinib-plus-pembrolizumab than with sunitinib treatment, notably among patients with baseline bone metastases (HR 0.33, 95% CI 0.21–0.52) and patients with sarcomatoid features (HR 0.39, 95% CI 0.18–0.84). Median OS favored lenvatinib plus pembrolizumab over sunitinib irrespective of metastatic lesions at baseline, prior nephrectomy, and sarcomatoid features. Of interest, among patients with baseline bone metastases the HR for survival was 0.50 (95% CI 0.30–0.83) and among patients with sarcomatoid features the HR for survival was 0.91 (95% CI 0.32–2.58); though for many groups, median OS was not reached. ORR also favored lenvatinib plus pembrolizumab over sunitinib across all subgroups; similarly, complete responses also followed this pattern.

Conclusion

Efficacy outcomes improved following treatment with lenvatinib-plus-pembrolizumab versus sunitinib in patients with RCC—irrespective of the presence or absence of baseline lung metastases, baseline bone metastases, baseline liver metastases, prior nephrectomy, or sarcomatoid features. These findings corroborate those of the primary CLEAR study analysis in the overall population and support lenvatinib plus pembrolizumab as a standard of care in 1L treatment for patients with advanced RCC.

Clinical trial registration

ClinicalTrials.gov, identifier NCT02811861
Genomic signatures and prognosis of advanced stage Chinese pediatric T cell lymphoblastic lymphoma by whole exome sequencing
Authors: Liu Qinglin , Yu Xiang , Wen Jinquan , Yin Nange , Liao Xin , Zou Pinli , Guo Yuxia , Song Lin , Xiao Jianwen
Published: August 2023

Objective

To investigate the genomic signatures and prognosis of advanced-stage T cell lymphoblastic lymphoma (T-LBL) and to examine the relationship between T-LBL and T cell acute lymphoblastic leukemia (T-ALL).

Methods

35 Chinese T-LBL children with stage III or IV disease were recruited for this study. They were treated with combination chemotherapy and whole exome sequencing. The relationship of the clinical features, prognosis and specific gene mutations was researched. Gene chips of T-LBL and T-ALL were downloaded from a database, and differential gene expression was analyzed.

Results

Germline causal gene mutations (CARS or MAP2K2) were detected in 2 patients; 3.06 ± 2.21 somatic causal gene mutations were identified in the 35 patients, and somatic mutations were observed in the NOTCH1, FBXW7, PHF6 and JAK3 genes. NOTCH1 mutations were signiﬁcantly associated with FBXW7 mutations, and the age at diagnosis of patients with NOTCH1-FBXW7 mutations was less than that of patients without such mutations (P < 0.05). 32 patients achieved complete remission (CR), and 14 and 18 patients were classified into the intermediate risk (IR) group and high risk (HR) group. During a median follow-up of 44 months, 3 patients relapsed. Three-year prospective event free survival (pEFS) was 82.286%, and no significant differences of pEFS were found for different sexes, ages, or statuses of NOTCH1-FBXW7 mutations, (P > 0.05); however, the mean survival time of the IR group was longer than that of the HR group (P < 0.05). Differential expression of genes in the T-LBL and/or T-ALL datasets was analyzed using the R package limma, and 1/3 of the differentially expressed genes were found in both the T-ALL and T-LBL datasets. High expression of PI3K-Akt signal pathway genes and the USP34 gene was found in the T-LBL dataset.

Conclusion

Although T-ALL and T-LBL both originate from precursor T-cells and are considered different manifestations of the same disease and the outcome of T-LBL is favorable when using T-ALL-based chemotherapy, there are differences in the gene distribution between T-LBL and T-ALL. It seems that the PI3K-Akt signaling pathway and the USP34 gene play important roles in T-LBL, but medicines targeting the USP34 gene or the PI3K-Akt pathway may be invalid.
Characterizing indigenous plant growth promoting bacteria and their synergistic effects with organic and chemical fertilizers on wheat (Triticum aestivum)
Authors: Asghar Israr , Ahmed Maqsood , Farooq Muhammad Ansar , Ishtiaq Muhammad , Arshad Muhammad , Akram Muhammad , Umair Adnan , Alrefaei Abdulwahed Fahad , Jat Baloch Muhammad Yousuf , Naeem Aamna
Published: August 2023

The excessive use of chemical fertilizers is deteriorating both the environment and soil, making it a big challenge faced by sustainable agriculture. To assist the efforts for the solution of this burning issue, nine different potential native strains of plant growth-promoting bacteria (PGPB) namely, SA-1(Bacillus subtilis), SA-5 (Stenotrophomonas humi),SA-7(Azospirillum brasilense), BH-1(Azospirillum oryzae), BH-7(Azotobacter armeniacus), BH-8(Rhizobium pusense), BA-3(Azospirillum zeae), BA-6(Rhizobium pusense), and BA-7(Pseudomonas fragi) were isolated that were characterized morphologically, biochemically and molecularly on the basis of 16S rRNA sequencing. Furthermore, the capability of indigenous PGPB in wheat (Triticum aestivum, Chakwal-50) under control, DAP+FYM, SA-1,5,7, BH-1,7,8, BA-3,6,7, DAP+ FYM + SA-1,5,7, DAP+FYM+ BH-1,7,8 and DAP+FYM+ BA-3,6,7 treatments was assessed in a randomized complete block design (RCBD). The results of the study showed that there was a significant increase in plant growth, nutrients, quality parameters, crop yield, and soil nutrients at three depths under SA-1,5,7, BH-1,7,8, and BA-3,6,7 in combination with DAP+FYM. Out of all these treatments, DAP+ FYM + BA-3,6,7 was found to be the most efficient for wheat growth having the highest 1000-grain weight of 55.1 g. The highest values for plant height, no. of grains/spike, spike length, shoot length, root length, shoot dry weight, root dry weight, 1000 grain weight, biological yield, and economic yield were found to be 90.7 cm, 87.7 cm, 7.20 cm, 53.5 cm, 33.5 cm, 4.87 g, 1.32 g, 55.1 g, 8209 kg/h, and 4572 kg/h, respectively, in the DAP+FYM+BA treatment. The DAP+FYM+BA treatment had the highest values of TN (1.68 µg/mL), P (0.38%), and K (1.33%). Likewise, the value of mean protein (10.5%), carbohydrate (75%), lipid (2.5%), and available P (4.68 ppm) was also highest in the DAP+FYM+BA combination. C:P was found to be significantly highest (20.7) in BA alone but was significantly lowest (11.9) in DAP+FYM+BA. Hence, the integration of strains BA-3, BA-5, and BA-7 in fertilizers can be regarded as the most suitable choice for agricultural growth in the sub-mountainous lower region of AJK. This could serve as the best choice for sustainable wheat growth and improved soil fertility with lesser impacts on the environment.
Enhancing host-pathogen phenotyping dynamics: early detection of tomato bacterial diseases using hyperspectral point measurement and predictive modeling
Authors: Reis Pereira Mafalda , Santos Filipe Neves dos , Tavares Fernando , Cunha Mário
Published: August 2023

Early diagnosis of plant diseases is needed to promote sustainable plant protection strategies. Applied predictive modeling over hyperspectral spectroscopy (HS) data can be an effective, fast, cost-effective approach for improving plant disease diagnosis. This study aimed to investigate the potential of HS point-of-measurement (POM) data for in-situ, non-destructive diagnosis of tomato bacterial speck caused by Pseudomonas syringae pv. tomato (Pst), and bacterial spot, caused by Xanthomonas euvesicatoria (Xeu), on leaves (cv. cherry). Bacterial artificial infection was performed on tomato plants at the same phenological stage. A sensing system composed by a hyperspectral spectrometer, a transmission optical fiber bundle with a slitted probe and a white light source were used for spectral data acquisition, allowing the assessment of 3478 spectral points. An applied predictive classification model was developed, consisting of a normalizing pre-processing strategy allied with a Linear Discriminant Analysis (LDA) for reducing data dimensionality and a supervised machine learning algorithm (Support Vector Machine – SVM) for the classification task. The predicted model achieved classification accuracies of 100% and 74% for Pst and Xeu test set assessments, respectively, before symptom appearance. Model predictions were coherent with host-pathogen interactions mentioned in the literature (e.g., changes in photosynthetic pigment levels, production of bacterial-specific molecules, and activation of plants’ defense mechanisms). Furthermore, these results were coherent with visual phenotyping inspection and PCR results. The reported outcomes support the application of spectral point measurements acquired in-vivo for plant disease diagnosis, aiming for more precise and eco-friendly phytosanitary approaches.
Metabolic syndrome in patients with obsessive-compulsive disorder
Authors: Khalkhali Mohammadrasoul , Rasekh Kiarash , Eslamdoust-Siahestalkhi Fatemeh , Farrahi Hassan , Zare Roghaye
Published: August 2023

Objective

Metabolic syndrome (MetS) is a collection of chemical and clinical risk factors. Patients with obsessive-compulsive disorder (OCD) might be at risk of MetS. This study aimed to investigate the prevalence and clinical correlates of MetS in an Iranian clinical sample of patients with OCD.

Methods

We included 107 patients with OCD in a cross-sectional study. Demographic and clinical characteristics including OC symptoms, duration of treatment, age of onset, medications history, and comorbidity with other psychiatric disorders were collected.

Results

The prevalence of MetS was 39.2%. Abdominal obesity was the most frequent component of MetS (68.2%), followed by low high-density lipoprotein cholesterol (50.5%). High serum triglycerides, high fasting serum glucose, high systolic blood pressure, and high diastolic blood pressure were observed in 47.7, 20.6, 18.7, and 9.3% of patients, respectively. Patients with MetS were older, married, had a low education level, had a high body mass index, and had no aggressive OC symptoms. MetS was not associated with psychiatric disorders comorbidities, age of onset, and duration of treatment.

Conclusion

The results of this study were in line with the results of other studies that reported the poor health status of patients with OCD. A large number of patients are affected or are at risk of developing MetS. These patients need medical care along with the usual OCD treatments.
Applications of indocyanine green (ICG) fluorescence technology in open surgery: preliminary experience in pediatric surgery
Authors: Esposito Ciro , Lepore Benedetta , Cerulo Mariapina , Del Conte Fulvia , Coppola Vincenzo , Esposito Giovanni , Carulli Roberto , Carraturo Francesca , Escolino Maria
Published: August 2023

Background

Indocyanine green fluorescence technology (ICG) in pediatric minimally invasive surgery has undergone an important improvement in the last 5 years. However, its use in open surgery is still limited. In this paper, we aim to report our preliminary experience with Rubina® lens ICG fluorescence technology in combination with the IMAGE1 S™ system from KARL STORZ in open excision of masses in children.

Methods

The records of 18 patients undergoing open surgery for head, neck and thorax masses between September and November 2022 were retrospectively reviewed. Rubina® lens ICG fluorescence technology system was used in all the cases. In 10 cases we adopted the holding arm system and in 8 cases the hand-held technique. Data about patients' demographics, surgery and outcomes were collected and analyzed through the following criteria: mass localization, intraoperative time (min), ICG administration (ml), intraoperative complications, postoperative complications.

Results

A total of 18 patients were operated: 4 thyroglossal duct cysts, 3 supraorbital cysts, 2 neck masses, 2 pre-auricular and 2 scalp cysts, 2 gynecomastias, 2 lymphangiomas, 1 nose mass. In all the cases, intralesional injection of 0.5–1 ml of ICG solution was performed peri-operatively. Mean operative time was 58.4 min (35–134 min). Postoperative complications included seroma formation in 2 cases. Surgical pathology reports confirmed complete mass excision in all the cases.

Conclusion

Based on our preliminary experience, ICG fluorescence guided surgery using Rubina® lens system was very helpful also in open surgery procedures. Rubina® lens system permits to have a very low complication rate, a time-saving surgery, a real time reliability of anatomic structures and an excellent clinical safety. In our experience, holding arm system seems more comfortable than hand-held system. However, further cases need to be performed to evaluate the exact role and to identify new indications of this technique in open pediatric surgical procedures.
Crew Interaction in Extended Space Missions
Authors: Vinokhodova Alla , Gushin Vadim , Kuznetsova Polina , Yusupova Anna
Published: August 2023

Detection of the extent of common values in a cohesive space crew has become an important trend in modern space psychology. It is known from the works of Ch. Osgood that the semantic differential scale is a reliable way to obtain objective information on the emotional attitudes towards a topic of interest. Within the frame of the Russian space experiment “Interactions” on the International Space Station (ISS), a computerized survey, the Personal Self-Perception and Attitudes (PSPA), was developed for analyzing the subjects’ emotional attitudes toward their social environment. In the course of the PSPA procedure, the crewmembers rate each other and themselves (in the past, present, and future) using the criteria previously personally chosen. These criteria should be regarded as their personal values. A total of 30 subjects have already completed the study on board the ISS. The main tasks of the study are: (1) to define individual and group values and the extent of group identification reflected in sharing these values; (2) to determine the impact of cross-cultural factors on mutual perceptions and self-perceptions in space crews and with the Mission Control Center (MCC); (3) to study changes in the space crews’ group cohesiveness and structure as they are exposed to the stress of the extended space mission environment. The data obtained indicate an increase in a “psychological distance” between the crew and the MCC personnel versus increased crew cohesion. The results gained made it possible to identify the most significant categories of values common to the subjects from the professional cosmonaut group. The priority of these shared values for each subject is an important condition for the formation of a cohesive crew.
Study on Failure Criteria and the Numerical Simulation Method of a Coal-Based Carbon Foam under Multiaxial Loading
Authors: Zhuang Qikai , Cheng Xiaoquan , Yue Peijie , Guo Xin , Li Kai
Published: August 2023

Coal-based carbon foam (CCF) has broad application prospects in aerospace, composite material tooling and other fields. However, the lack of failure criteria limits its promotion. In previous studies, the failure criteria of similar materials were proposed, but there are some limitations. This paper proposes improved failure criteria based on macro-mechanical tests. Furthermore, uniaxial and multiaxial loading tests were carried out to obtain accurate failure criteria of CCF. Finally, 3-points bending tests of the CCF sandwich structure were conducted and their finite element models (FEMs) were established. The CCF test results show that the mechanical properties of CCF are transversely isotropic. The failure criteria in this paper can accurately predict the stress when the CCF fails. The error band boundary formula caused by the dispersion of the material were also given. The maximum load Pmax calculated by the failure surface (3684 N) was only 4.7% larger than the mean value measured by the test (3518 N), and all of the Pmax measured by the test (3933 N, 3640 N, 3657 N, 3269 N, 3091 N) were between the maximum value (4297 N) and minimum value (3085 N) calculated by the error band boundary formula, which means that the failure criteria have good precision.
Detection and Positioning of Camellia oleifera Fruit Based on LBP Image Texture Matching and Binocular Stereo Vision
Authors: Lei Xiangming , Wu Mingliang , Li Yajun , Liu Anwen , Tang Zhenhui , Chen Shang , Xiang Yang
Published: August 2023

To achieve the rapid recognition and accurate picking of Camellia oleifera fruits, a binocular vision system composed of two industrial cameras was used to collect images of Camellia oleifera fruits in natural environments. The YOLOv7 convolutional neural network model was used for iterative training, and the optimal weight model was selected to recognize the images and obtain the anchor frame region of the Camellia oleifera fruits. The local binary pattern (LBP) maps of the anchor frame region were extracted and matched by using the normalized correlation coefficient template matching algorithm to obtain the positions of the center point in the left and right images. The recognition experimental results showed that the accuracy rate, recall rate, mAP and F1 of the model were 97.3%, 97.6%, 97.7% and 97.4%. The recognition rate of the Camellia oleifera fruit with slight shading was 93.13%, and the recognition rate with severe shading was 75.21%. The recognition rate of the Camellia oleifera fruit was 90.64% under sunlight condition, and the recognition rate was 91.34% under shading condition. The orchard experiment results showed that, in the depth range of 400–600 mm, the maximum error value of the binocular stereo vision system in the depth direction was 4.279 mm, and the standard deviation was 1.142 mm. The detection and three-dimensional positioning accuracy of the binocular stereo vision system for Camellia oleifera fruits could basically meet the working requirements of the Camellia oleifera fruit-picking robot.
Transcriptomic and Metabolic Analyses Elucidate the Metabolomic Variation in Leaf Development of a Calcium-Rich Vegetable (Primulina eburnea)
Authors: Zhang Yi , Yang Endian , Liu Qin , Feng Chen
Published: August 2023

Primulina eburnea, a plant with a longstanding history of traditional medicinal use, has emerged as a novel calcium-rich vegetable characterized by a remarkable abundance of soluble and bioavailable calcium within its leaves. However, most of the metabolites produced by P. eburnea have not been identified, and few studies have addressed the accumulation of nutritional and medicinal ingredients during leaf development. In the present study, we investigated the “Gaogai-1” cultivar by integrating transcriptomic and mestabolomic methods. A total of 1041 metabolites were identified in calcium-rich vegetable leaves. During leaf development, most of the flavor components, including amino acids and derivatives, organic acids and derivatives, and carbohydrates, decreased their content, which contrasted with the starch content. Most of the antioxidant components, e.g., flavonoids, alkaloids, vitamins, and phenolamines, were more highly accumulated in the bud leaves than in the mature leaves, but terpenes had the opposite trend. These results indicate that leaves of different developmental stages are suitable for different medicinal uses and that P. eburnea could be regarded as a new type of medicinal and edible vegetable. Furthermore, most of the coding genes related to the important components that influence the flavor and nutritional and medicinal values were identified, and their expressions were consistent with the component accumulation profiles. Transcription factors that may regulate the coding genes were identified by combining the co-expression network and cis-element binding ability prediction. The high level of water-soluble calcium was maintained by the high expression of Ca2+/cation antiporter genes in calcium-rich vegetable leaves. Our results uncovered metabolomic and molecular evidence of the mechanisms of nutritional and medicinal component accumulation underlying the leaf development of a calcium-rich vegetable. This study provides a wealth of data for the future utilization and improvement of calcium-rich vegetable cultivars.
Effects of Paclobutrazol and Mepiquat Chloride on the Physiological, Nutritional, and Morphological Behavior of Potted Icterina Sage under Greenhouse Conditions
Authors: Bañón Daniel , Ortuño María Fernanda , Sánchez-Blanco María Jesús , Pagán Beatriz Lorente , Bañón Sebastián
Published: August 2023

Plant growth regulators (PGRs) are commonly used in horticulture to improve crop quality, save water, and enhance plant resilience to stress. In this study, we examined the effects of two PGRs, paclobutrazol (PBZ) and mepiquat chloride (MC), on the growth and health of Salvia officinalis ‘Icterina’, a popular ornamental and aromatic plant. Parameters such as growth and development, water status, chlorophyll levels, nutrient content, photosynthetic performance, and gas exchange were evaluated. The study took place in a greenhouse with automatic watering and three plant groups: one treated with PBZ, another with MC, and a control group (untreated). Only one application of growth retardants was made, with 0.1 L per pot of a 100 mg/L solution of PBZ, and 0.1 L per pot of a 2.5 g/L solution of MC. The results showed that both PBZ and MC treatments reduced the plant’s water consumption, with PBZ being more effective in limiting leaf growth and promoting the accumulation of substances in the leaves. Both PGRs resulted in smaller plants, reducing the need for soil and potting materials. The MC treatment improved nutrient absorption, reducing the requirement for fertilizers. When subjected to environmental stress from March to June in the greenhouse, Salvia plants benefited from the application of both PGRs, as they helped maintain photosynthetic activity. These findings contribute to improving the sustainability of nursery practices by utilizing PGRs to conserve resources and mitigate the impact of stressful environmental conditions on sage plants.
Intriguing Aspects of Polar-to-Tropical Mesospheric Teleconnections during the 2018 SSW: A Meteor Radar Network Study
Authors: Eswaraiah Sunkara , Seo Kyong-Hwan , Kumar Kondapalli Niranjan , Koval Andrey V. , Ratnam Madineni Venkat , Mengist Chalachew Kindie , Chalapathi Gasti Venkata , Liu Huixin , Kwak Young-Sil , Merzlyakov Eugeny , Jacobi Christoph , Kim Yong-Ha , Rao Sarangam Vijaya Bhaskara , Mitchell Nicholas J.
Published: August 2023

Using a network of meteor radar observations, observational evidence of polar-to-tropical mesospheric coupling during the 2018 major sudden stratosphere warming (SSW) event in the northern hemisphere is presented. In the tropical lower mesosphere, a maximum zonal wind reversal (−24 m/s) is noted and compared with that identified in the extra-tropical regions. Moreover, a time delay in the wind reversal between the tropical/polar stations and the mid-latitudes is detected. A wide spectrum of waves with periods of 2 to 16 days and 30–60 days were observed. The wind reversal in the mesosphere is due to the propagation of dominant intra-seasonal oscillations (ISOs) of 30–60 days and the presence and superposition of 8-day period planetary waves (PWs). The ISO phase propagation is observed from high to low latitudes (60° N to 20° N) in contrast to the 8-day PW phase propagation, indicating the change in the meridional propagation of winds during SSW, hence the change in the meridional circulation. The superposition of dominant ISOs and weak 8-day PWs could be responsible for the delay of the wind reversal in the tropical mesosphere. Therefore, this study has strong implications for understanding the reversed (polar to tropical) mesospheric meridional circulation by considering the ISOs during SSW.
Localization of Colorectal Cancer Lesions in Contrast-Computed Tomography Images via a Deep Learning Approach
Authors: Sahoo Prasan Kumar , Gupta Pushpanjali , Lai Ying-Chieh , Chiang Sum-Fu , You Jeng-Fu , Onthoni Djeane Debora , Chern Yih-Jong
Published: August 2023

Abdominal computed tomography (CT) is a frequently used imaging modality for evaluating gastrointestinal diseases. The detection of colorectal cancer is often realized using CT before a more invasive colonoscopy. When a CT exam is performed for indications other than colorectal evaluation, the tortuous structure of the long, tubular colon makes it difficult to analyze the colon carefully and thoroughly. In addition, the sensitivity of CT in detecting colorectal cancer is greatly dependent on the size of the tumor. Missed incidental colon cancers using CT are an emerging problem for clinicians and radiologists; consequently, the automatic localization of lesions in the CT images of unprepared bowels is needed. Therefore, this study used artificial intelligence (AI) to localize colorectal cancer in CT images. We enrolled 190 colorectal cancer patients to obtain 1558 tumor slices annotated by radiologists and colorectal surgeons. The tumor sites were double-confirmed via colonoscopy or other related examinations, including physical examination or image study, and the final tumor sites were obtained from the operation records if available. The localization and training models used were RetinaNet, YOLOv3, and YOLOv8. We achieved an F1 score of 0.97 (±0.002), a mAP of 0.984 when performing slice-wise testing, 0.83 (±0.29) sensitivity, 0.97 (±0.01) specificity, and 0.96 (±0.01) accuracy when performing patient-wise testing using our derived model YOLOv8 with hyperparameter tuning.
Discrimination and Quantification of Glutathione by Cu+-Based Nanozymes
Authors: Liu Meixuan , Yan Chen , Ye Qianyun , Sun Xiaohuan , Han Jie
Published: August 2023

Glutathione (GSH) is the most abundant low-molecular-weight biological thiol in vivo and has been linked to several diseases. The accurate quantification of GSH is therefore crucial for disease diagnosis and monitoring. In this study, we prepared self-assembled Cu(I)-Cys (cysteine) nanozymes through a two-step procedure. The Cu(I)-Cys nanoparticles exhibited peroxidase-mimicking activity. Upon the addition of H2O2, they were able to oxidize 3,3,5,5-tetramethylbenzidine (TMB) into oxTMB, resulting in a measurable increase in UV-Vis absorption at 655 nm. However, in the presence of GSH, oxTMB was reduced back to TMB, leading to a decrease in UV-Vis absorption at 655 nm. By utilizing these changes in the absorption intensity, we achieved the sensitive detection of GSH with a detection limit of 2.13 μM. Moreover, taking advantage of the different peroxidase-mimicking activities of Cu(I)-Cys nanoparticles at various pH values, a sensor array with Cu(I)-Cys nanoparticles at pH 4 and pH 5 was constructed. The discrimination of GSH among Cys and ascorbic acid was achieved and the practicability of the sensor array in human serum was validated. This novel approach holds significant promise for the precise discrimination and quantification of GSH and its potential applications in disease diagnosis and therapeutics.