bluuebunny/crossref_metadata_2025 huggingface dataset:
Adsorption Behavior of 3-phenoxybenzoic Acid by Lactobacillus Plantarum and Its Potential Application in Simulated Digestive Juices
Authors: Li Jianlong , Hu Kaidi , Hu Lu , Hou Xiaoyan , Li Qin , Liu Aiping , Chen Shujuan , Ao Xiaolin , Hu Xinjie , He Li , Tang Huaqiao , Huang Daomei , Yang Yong , Zou Likou , Liu Shuliang
Published: May 2022

3-PBA is a major degradation intermediate of pyrethroids. Its widespread existence in the environment poses a severe threat to the ecosystem and human health. This study evaluated the adsorption capacity of L. plantarum RS20 toward 3-PBA. Batch adsorption experiments indicated that the optimal adsorption conditions were a temperature of 37 °C and initial pH of 6.0–8.0, under which the removal rate was positively correlated with the cell concentration. In addition, there was no link between the incubation time and adsorption rate. The kinetic study showed that the adsorption process fitted well with the pseudo-second-order model, and the adsorption isotherms could be described by both Langmuir and Freundlich equations. Heat and acid treatments showed that the ability of strain RS20 in removing 3-PBA was independent of microbial vitality. Indeed, it was involved with chemisorption and physisorption via the cell walls. The cell walls made the highest contribution to 3-PBA removal, according to the adsorption experiments using different cellular components. This finding was further reconfirmed by SEM. FTIR spectroscopy analysis indicated that carboxyl, hydroxyl, amino groups, and –C–N were the functional sites for the binding of 3-PBA. The co-culture experiments showed that the adsorption of strain RS20 enhanced the degradation of 3-PBA by strain SC-1. Strain RS20 could also survive and effectively remove 3-PBA in simulated digestive juices. Collectively, strain RS20 could be employed as a biological detoxification agent for humans and animals by eliminating 3-PBA from foods, feeds, and the digestive tract in the future.
In-Silico Selection of Aptamer Targeting SARS-CoV-2 Spike Protein
Authors: Lin Yu-Chao , Chen Wen-Yih , Hwu En-Te , Hu Wen-Pin
Published: May 2022

Aptamers are single-stranded, short DNA or RNA oligonucleotides that can specifically bind to various target molecules. To diagnose the infected cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in time, numerous conventional methods are applied for viral detection via the amplification and quantification of DNA or antibodies specific to antigens on the virus. Herein, we generated a large number of mutated aptamer sequences, derived from a known sequence of receptor-binding domain (RBD)-1C aptamer, specific to the RBD of SARS-CoV-2 spike protein (S protein). Structural similarity, molecular docking, and molecular dynamics (MD) were utilized to screen aptamers and characterize the detailed interactions between the selected aptamers and the S protein. We identified two mutated aptamers, namely, RBD-1CM1 and RBD-1CM2, which presented better docking results against the S protein compared with the RBD-1C aptamer. Through the MD simulation, we further confirmed that the RBD-1CM1 aptamer can form the most stable complex with the S protein based on the number of hydrogen bonds formed between the two biomolecules. Based on the experimental data of quartz crystal microbalance (QCM), the RBD-1CM1 aptamer could produce larger signals in mass change and exhibit an improved binding affinity to the S protein. Therefore, the RBD-1CM1 aptamer, which was selected from 1431 mutants, was the best potential candidate for the detection of SARS-CoV-2. The RBD-1CM1 aptamer can be an alternative biological element for the development of SARS-CoV-2 diagnostic testing.
The Metabolism of a Novel Cytochrome P450 (CYP77B34) in Tribenuron-Methyl-Resistant Descurainia sophia L. to Herbicides with Different Mode of Actions
Authors: Shen Jing , Yang Qian , Hao Lubo , Zhang Lingling , Li Xuefeng , Zheng Mingqi
Published: May 2022

Descurainia sophia L. (flixweeds) is a noxious broad-leaf weed infesting winter wheat fields in China that has evolved high resistance to tribenuron-methyl. In this work, a brand new gene CYP77B34 was cloned from tribenuron-methyl-resistant (TR) D. sophia and transferred into Arabidopsis thaliana, and the sensitivities of Arabidopsis with or without the CYP77B34 transgene to herbicides with a different mode of actions (MoAs) were tested. Compared to Arabidopsis expressing pCAMBIA1302-GFP (empty plasmid), Arabidopsis transferring pCAMBIA1302-CYP77B34 (recombinant plasmid) became resistant to acetolactate synthase (ALS)-inhibiting herbicide tribenuron-methyl, protoporphyrinogen oxidase (PPO)-inhibiting herbicides carfentrazone-ethyl and oxyfluorfen. Cytochrome P450 inhibitor malathion could reverse the resistance to tribenuron-methyl, carfentrazone-ethyl and oxyfluorfen in transgenic Arabidopsis plants. In addition, the metabolic rates of tribenuron-methyl in Arabidopsis expressing CYP77B34 were significantly higher than those in Arabidopsis expressing pCAMBIA1302-GFP. Other than that, the transgenic plants showed some tolerance to very-long-chain fatty acid synthesis (VLCFAs)-inhibiting herbicide pretilachlor and photosystem (PS) II-inhibiting herbicide bromoxynil. Subcellular localization revealed that the CYP77B34 protein was located in the endoplasmic reticulum (ER). These results clearly indicated that CYP77B34 mediated D. sophia resistance to tribenuron-methyl and may have been involved in D. sophia cross-resistance to carfentrazone-ethyl, oxyfluorfen, pretilachlor and bromoxynil.
Facial Emotion Recognition Using Conventional Machine Learning and Deep Learning Methods: Current Achievements, Analysis and Remaining Challenges
Authors: Khan Amjad Rehman
Published: May 2022

Facial emotion recognition (FER) is an emerging and significant research area in the pattern recognition domain. In daily life, the role of non-verbal communication is significant, and in overall communication, its involvement is around 55% to 93%. Facial emotion analysis is efficiently used in surveillance videos, expression analysis, gesture recognition, smart homes, computer games, depression treatment, patient monitoring, anxiety, detecting lies, psychoanalysis, paralinguistic communication, detecting operator fatigue and robotics. In this paper, we present a detailed review on FER. The literature is collected from different reputable research published during the current decade. This review is based on conventional machine learning (ML) and various deep learning (DL) approaches. Further, different FER datasets for evaluation metrics that are publicly available are discussed and compared with benchmark results. This paper provides a holistic review of FER using traditional ML and DL methods to highlight the future gap in this domain for new researchers. Finally, this review work is a guidebook and very helpful for young researchers in the FER area, providing a general understating and basic knowledge of the current state-of-the-art methods, and to experienced researchers looking for productive directions for future work.
Shortest Path Algorithms for Pedestrian Navigation Systems
Authors: Koritsoglou Kyriakos , Tsoumanis Georgios , Patras Vaios , Fudos Ioannis
Published: May 2022

Efficient shortest path algorithms are of key importance for routing and navigation systems. However, these applications are designed focusing on the requirements of motor vehicles, and therefore, finding paths in pedestrian sections of urban areas is not sufficiently supported. In addition, finding the shortest path is often not adequate for urban sidewalk routes, as users of these applications may also be interested in alternative routes that, although slightly longer, possess other desirable features and properties. According to the literature, the search for alternative routes is carried out mainly using the k-shortest paths (KSP) algorithm which represents an ordered list of all available alternatives. Even though various KSP algorithms have been proposed, to the best of our knowledge, there is no research addressing all issues inherent in a pedestrian navigation system. The purpose of this paper is to present a heuristic algorithm for graph datasets that implements a penalty-based method which, by increasing certain edge weights, effectively searches for the most accessible alternative paths in multi-route cases. To demonstrate how the algorithm works, we present experimental results on finding the most accessible paths in pedestrian sections of the historical center of Thessaloniki city.
TiO2-La2O3 as Photocatalysts in the Degradation of Naproxen
Authors: Marizcal-Barba Adriana , Limón-Rocha Isaias , Barrera Arturo , Casillas José Eduardo , González-Vargas O. A. , Rico José Luis , Martinez-Gómez Claudia , Pérez-Larios Alejandro
Published: May 2022

The indiscriminate use of naproxen as an anti-inflammatory has been the leading cause of pollution in sewage effluents. Conversely, titanium dioxide is one of the most promising photocatalyst for the degradation of pollutants. Ti-La mixed oxides containing 0, 1, 3, 5, and 10 wt.% of lanthanum were synthetized by sol-gel and tested as photocatalysts in the degradation of naproxen (NPX). The materials were further characterized by X-ray diffraction (XRD), nitrogen physisorption (BET), scanning electron microscopy (SEM), UV-Vis and Fourier-transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). The XRD patterns resembled that of anatase titania. The Eg values, determined from the UV-Vis spectra, vary from 2.07 to 3.2 eV corresponded to pure titania. The photocatalytic activity of these materials showed a degradation of naproxen from 93.6 to 99.8 wt.% after 4 h under UV irradiation.
New Trends on Vanadium Chemistry, Biochemistry, and Medicinal Chemistry
Authors: Gambino Dinorah
Published: May 2022

Vanadium was discovered twice [...]
Characterization and Outcomes of SARS-CoV-2 Infection in Overweight and Obese Patients: A Dynamic Comparison of COVID-19 Pandemic Waves
Authors: Fericean Roxana Manuela , Citu Cosmin , Manolescu Diana , Rosca Ovidiu , Bratosin Felix , Tudorache Emanuela , Oancea Cristian
Published: May 2022

There are few data on the dynamics of SARS-CoV-2 viral manifestations in obese and overweight persons during each of the five waves that occurred in Romania during the last two years. As such, the purpose of this research was to characterize the variance in case severity, symptomatology, ICU hospitalizations, and mortality among overweight and obese individuals infected with the SARS-CoV-2 virus. We included 250 overweight and obese patients admitted to hospital with COVID-19, where 50 patients were selected from each of the five pandemic waves that existed in Romania until March 2022. A total of 113 patients with normal body mass index were included in the study. They were matched with overweight and obese patients by age, gender, and cardiovascular comorbidities to avoid the effect of confounding factors. Between the five waves of the COVID-19 pandemic in Romania, the present investigation found substantial changes in overweight and obese patient features. Obesity increases the risk of hospitalization, severe complications, and mortality from COVID-19. However, this unique demographic is disproportionately affected by obesity-related comorbidities, which contribute to these adverse outcomes. We advocate for the development of new guiding principles for the formulation of healthcare strategies aimed at high-prevalence special populations such as overweight and obese individuals, while also promoting pandemic containment and avoiding the recurrence of pandemic waves with the same guidelines that proved detrimental in terms of economic and human life loss.
Risk of Adverse Pregnancy Outcomes for Women with IBD in an Expert IBD Antenatal Clinic
Authors: Lever Gillian , Chipeta Hlupekile , Glanville Tracey , Selinger Christian
Published: May 2022

Background: Patients with inflammatory bowel disease (IBD) are at increased risk of adverse outcomes from pregnancy. It is unclear whether IBD indications account for the higher rate of Caesarean section (CS) in IBD patients. Methods: A retrospective cohort study of 179 IBD patients cared for in a dedicated combined IBD antenatal clinic and 31,528 non-IBD patients was performed. The outcomes were method of delivery, preterm birth, birthweight, admission to neonatal intensive care unit (NICU), and stillbirth. We analysed the associations between disease activity, medication with method of delivery, and neonatal outcomes. Results: Delivery by CS was more common in IBD patients (RR 1.45, CI 1.16–1.81, p = 0.0021); emergency CS delivery was equally likely (RR 1.26, CI 0.78–2.07, p = 0.3). Forty percent of elective CS were performed for IBD indications. Stillbirth was five-fold higher in IBD patients (RR 5.14, CI 1.92–13.75, p < 0.001). Preterm delivery, low birthweight, and admission to NICU were not more common in patients with IBD, and IBD medications did not increase these risks. Active disease during pregnancy was not associated with adverse outcomes. Conclusions: Delivery by CS was more frequent in the IBD cohort, and most elective CSs were obstetrically indicated. A dedicated IBD antenatal clinic providing closer monitoring and early detection of potential issues may help improve outcomes.
Innate and Acquired Cellular Immunity in Children with Familial Hypercholesterolemia Treated with Simvastatin
Authors: Motkowski Radosław , Alifier Marek , Abramowicz Paweł , Konstantynowicz Jerzy , Mikołuć Bożena , Stasiak-Barmuta Anna
Published: May 2022

The aim of this cross-sectional study was to assess the influence of simvastatin treatment in children with familial hypercholesterolemia (FH) on parameters of cellular immunity. Twenty-six children with FH were included, of which thirteen were treated with 10 mg simvastatin for at least 26 weeks, and thirteen were age- and sex-matched with a low-cholesterol diet only. Total WBC count and lipid profile were measured. Flow cytometry was used to identify lymphocyte subsets and determine the expression of adhesion molecules (AM) and toll-like receptors (TLRs) on leukocytes. No differences were found in the basic values of peripheral blood count and subpopulations of lymphocytes between groups. The percentage of granulocytes with the expression of AM was higher in those treated with statins. The TLR-2 expression on granulocytes and monocytes showed higher values, whereas the TLR-4 expression was lower on lymphocytes and granulocytes in simvastatin-treated children. Treatment with simvastatin in children with FH is not associated with alterations in the amounts of granulocytes and monocytes. There is no association between statin treatment and the pattern of peripheral blood lymphocyte subpopulations. The role of AM and TLRs needs further investigation, given the effect of statins on the innate immunity may be important for their efficacy and safety during growth.
The Impact of Lifestyle on Prostate Cancer: A Road to the Discovery of New Biomarkers
Authors: Leitão Catarina , Matos Bárbara , Roque Fátima , Herdeiro Maria Teresa , Fardilha Margarida
Published: May 2022

Prostate cancer (PCa) is one of the most common cancers among men, and its incidence has been rising through the years. Several risk factors have been associated with this disease and unhealthy lifestyles and inflammation were appointed as major contributors for PCa development, progression, and severity. Despite the advantages associated with the currently used diagnostic tools [prostate-specific antigen(PSA) serum levels and digital rectal examination (DRE)], the development of effective approaches for PCa diagnosis is still necessary. Finding lifestyle-associated proteins that may predict the development of PCa seems to be a promising strategy to improve PCa diagnosis. In this context, several biomarkers have been identified, including circulating biomarkers (CRP, insulin, C-peptide, TNFα-R2, adiponectin, IL-6, total PSA, free PSA, and p2PSA), urine biomarkers (PCA3, guanidine, phenylacetylglycine, and glycine), proteins expressed in exosomes (afamin, vitamin D-binding protein, and filamin A), and miRNAs expressed in prostate tissue (miRNA-21, miRNA-101, and miRNA-182). In conclusion, exploring the impact of lifestyle and inflammation on PCa development and progression may open doors to the identification of new biomarkers. The discovery of new PCa diagnostic biomarkers should contribute to reduce overdiagnosis and overtreatment.
The Incidence and Impact of In-Hospital Bleeding in Patients with Acute Coronary Syndrome during the COVID-19 Pandemic
Authors: Licordari Roberto , Sticchi Alessandro , Mancuso Filippo , Caracciolo Alessandro , Muscoli Saverio , Iacovelli Fortunato , Ruggiero Rossella , Scoccia Alessandra , Cammalleri Valeria , Pavani Marco , Loffi Marco , Scordino Domenico , Ferro Jayme , Rognoni Andrea , Buono Andrea , Nava Stefano , Albani Stefano , Colaiori Iginio , Zilio Filippo , Borghesi Marco , Regazzoni Valentina , Benenati Stefano , Pescetelli Fabio , De Marzo Vincenzo , Mannarini Antonia , Spione Francesco , Baldassarre Doronzo , De Benedictis Michele , Bonmassari Roberto , Danzi Gian Battista , Galli Mario , Ielasi Alfonso , Musumeci Giuseppe , Tomai Fabrizio , Pasceri Vincenzo , Porto Italo , Patti Giuseppe , Campo Gianluca , Colombo Antonio , Micari Antonio , Giannini Francesco , Costa Francesco
Published: May 2022

Background: The COVID-19 pandemic increased the complexity of the clinical management and pharmacological treatment of patients presenting with an Acute Coronary Syndrome (ACS). Aim: to explore the incidence and prognostic impact of in-hospital bleeding in patients presenting with ACS before and during the COVID-19 pandemic. Methods: We evaluated in-hospital Thrombolysis In Myocardial Infarction (TIMI) major and minor bleeding among 2851 patients with ACS from 17 Italian centers during the first wave of the COVID-19 pandemic (i.e., March–April 2020) and in the same period in the previous two years. Results: The incidence of in-hospital TIMI major and minor bleeding was similar before and during the COVID-19 pandemic. TIMI major or minor bleeding was associated with a significant threefold increase in all-cause mortality, with a similar prognostic impact before and during the COVID-19 pandemic. Conclusions: the incidence and clinical impact of in-hospital bleeding in ACS patients was similar before and during the COVID-19 pandemic. We confirmed a significant and sizable negative prognostic impact of in-hospital bleeding in ACS patients.
Association of Albumin-Corrected Serum Calcium Levels with Colorectal Cancer Survival Outcomes
Authors: Silveira Marina Nogueira , Pozzuto Lara , Mendes Maria Carolina Santos , da Cunha Lorena Pires , Costa Felipe Osório , Macedo Lígia Traldi , Brambilla Sandra Regina , Carvalheira José Barreto Campello
Published: May 2022

In epidemiological studies, higher calcium intake has been associated with decreased colorectal cancer (CRC) incidence. However, whether circulating calcium concentrations are associated with CRC prognosis is largely unknown. In this retrospective cohort analysis, we identified 498 patients diagnosed with stage I–IV CRC between the years of 2000 and 2018 in whom calcium and albumin level measurements within 3 months of diagnosis had been taken. We used the Kaplan–Meier method for survival analysis. We used multivariate Cox proportional hazards regression to identify associations between corrected calcium levels and CRC survival outcomes. Corrected calcium levels in the highest tertile were associated with significantly lower progression-free survival rates (hazard ratio (HR) 1.85; 95% confidence interval (CI) 1.28–2.69; p = 0.001) and overall survival (HR 1.86; 95% CI 1.26–2.74, p = 0.002) in patients with stage IV or recurrent CRC, and significantly lower disease-free survival rates (HR 1.44; 95% confidence interval (CI) 1.02–2.03; p = 0.040) and overall survival rates (HR 1.72; 95% CI 1.18–2.50; p = 0.004) in patients with stage I–III disease. In conclusion, higher corrected calcium levels after the diagnosis of CRC were significantly associated with decreased survival rates. Prospective trials are necessary to confirm this association.
Robotic Abdominal Surgery and COVID-19: A Systematic Review of Published Literature and Peer-Reviewed Guidelines during the SARS-CoV-2 Pandemic
Authors: Fleming Christina A. , Fullard Anna , Croghan Stefanie , Pellino Gianluca , Pata Francesco
Published: May 2022

Background: Significant concern emerged at the beginning of the SARS-CoV-2 pandemic regarding the safety and practicality of robotic-assisted surgery (RAS). We aimed to review reported surgical practice and peer-reviewed published review recommendations and guidelines relating to RAS during the pandemic. Methods: A systematic review was performed in keeping with PRISMA guidelines. This study was registered on Open Science Framework. Databases were searched using the following search terms: ‘robotic surgery’, ‘robotics’, ‘COVID-19’, and ‘SARS-CoV-2’. Firstly, articles describing any outcome from or reference to robotic surgery during the COVID-19/SARS-CoV-2 pandemic were considered for inclusion. Guidelines or review articles that outlined recommendations were included if published in a peer-reviewed journal and incorporating direct reference to RAS practice during the pandemic. The ROBINS-I (Risk of Bias in Non-Randomised Studies of Intervention) tool was used to assess the quality of surgical practice articles and guidelines and recommendation publications were assessed using the AGREE-II reporting tool. Publication trends, median time from submission to acceptance were reported along with clinical outcomes and practice recommendations. Results: Twenty-nine articles were included: 15 reporting RAS practice and 14 comprising peer-reviewed guidelines or review recommendations related to RAS during the pandemic, with multiple specialities (i.e., urology, colorectal, digestive surgery, and general minimally invasive surgery) covered. Included articles were published April 2020—December 2021, and the median interval from first submission to acceptance was 92 days. All surgical practice studies scored ‘low’ or ‘moderate’ risk of bias on the ROBINS-I assessment. All guidelines and recommendations scored ‘moderately well’ on the AGREE-II assessment; however, all underperformed in the domain of public and patient involvement. Overall, there were no increases in perioperative complication rates or mortalities in patients who underwent RAS compared to that expected in non-COVID practice. RAS was deemed safe, with recommendations for mitigation of risk of viral transmission. Conclusions: Continuation of RAS was feasible and safe during the SARS-CoV-2 pandemic where resources permitted. Post-pandemic reflections upon published robotic data and publication patterns allows us to better prepare for future events and to enhance urgent guideline design processes.
Mallampati Score Is an Independent Predictor of Active Oxygen Therapy in Patients with COVID-19
Authors: Dyrbuś Maciej , Oraczewska Aleksandra , Szmigiel Szymon , Gawęda Szymon , Kluszczyk Paulina , Cyzowski Tomasz , Jędrzejek Marek , Dubik Paweł , Kozłowski Michał , Kwiatek Sebastian , Celińska Beata , Wita Michał , Trejnowska Ewa , Swinarew Andrzej , Darocha Tomasz , Barczyk Adam , Skoczyński Szymon
Published: May 2022

Mallampati score has been identified and accepted worldwide as an independent predictor of difficult intubation and obstructive sleep apnea. We aimed to determine whether Mallampati score assessed on the first patient medical assessment allowed us to stratify the risk of worsening of conditions in patients hospitalized due to COVID-19. A total of 493 consecutive patients admitted between 13 November 2021 and 2 January 2022 to the temporary hospital in Pyrzowice were included in the analysis. The clinical data, chest CT scan, and major, clinically relevant laboratory parameters were assessed by patient-treating physicians, whereas the Mallampati score was assessed on admission by investigators blinded to further treatment. The primary endpoints were necessity of active oxygen therapy (AOT) during hospitalization and 60-day all-cause mortality. Of 493 patients included in the analysis, 69 (14.0%) were in Mallampati I, 57 (11.6%) were in Mallampati II, 78 (15.8%) were in Mallampati III, and 288 (58.9%) were in Mallampati IV. There were no differences in the baseline characteristics between the groups, except the prevalence of chronic kidney disease (p = 0.046). Patients with Mallampati IV were at the highest risk of AOT during the hospitalization (33.0%) and the highest risk of death due to any cause at 60 days (35.0%), which significantly differed from other scores (p = 0.005 and p = 0.03, respectively). Mallampati IV was identified as an independent predictor of need for AOT (OR 3.089, 95% confidence interval 1.65–5.77, p < 0.001) but not of all-cause mortality at 60 days. In conclusion, Mallampati IV was identified as an independent predictor of AOT during hospitalization. Mallampati score can serve as a prehospital tool allowing to identify patients at higher need for AOT.
Double-Blind, Randomized, Placebo-Controlled Study on hzVSF-v13, a Novel Anti-Vimentin Monoclonal Antibody Drug as Add-on Standard of Care in the Management of Patients with Moderate to Severe COVID-19
Authors: Prasenohadi Prasenohadi , Burhan Erlina , Dhunny Sri , Suharno Wahyuningsih , Wabnitz Paul , Kim Yoon-Won , Petrosillo Nicola
Published: May 2022

Humanized Virus Suppressing Factor-variant 13 (hzVSF-v13), a monoclonal IgG4 antibody against vimentin, was investigated in moderate to severe COVID-19 pneumonia through a Phase II study. Patients were randomized to two different IV doses of the test drug or saline with standard of care. Overall, 64 patients were recruited, and 62 entered the efficacy assessment in the full analysis set. Primary endpoint: The clinical failure rate at day 28 was 15.8% for placebo, 9.1% for low-dose hzVSF-v13 and 9.5% for high-dose hzVSF-v13 (not significant). A trend toward better efficacy was shown in several secondary endpoints, with statistical significance between low-dose hzVSF-v13 and placebo in terms of the rate of improved patients on the ordinal scale for clinical improvement (OSCI): 90.0% vs. 52.63% (p = 0.0116). In the severe stratum, the results of low-dose hzVSF-v13 vs. placebo were 90.0% and 22.2% for OSCI (p = 0.0092), 9 days and 14 days for time to discontinuation of oxygen therapy (p = 0.0308), 10 days and 15 days for both time to clinical improvement (TTCI) and time to recovery (TTR) and p = 0.0446 for both TTCI and TTR. Change from baseline of NEWS2 score at day 28 was −3.4 vs. + 0.4 (p = 0.0441). The results propose hzVSF-v13 as a candidate in the treatment of severe COVID-19.
Corneal Behavior during Tonometer Measurement during the Water Drinking Test in Eyes with XEN GelStent in Comparison to Non-Implanted Eyes
Authors: Jóźwik Agnieszka , Przeździecka-Dołyk Joanna , Wałek Ewa , Czerniak Magdalena , Asejczyk Magdalena
Published: May 2022

Biomechanics of the cornea have significant influences on the non-contact measurement of the intraocular pressure. The corneal behaviour during tonometry is a fundamental factor in estimating its value. The aim of the study was to analyse the behaviour of the cornea during tonometric measurement with the forced change in intraocular pressure during the water drinking test. Ocular Response Analyser (Reichert) was used to the measurement. Besides four basic parameters connected with intraocular pressure (IOPg, IOPcc) and biomechanics (corneal hysteresis CH and corneal resistance factor (CRF), other parameters representing the behaviour of the cornea during a puff of air were analysed. There were 47 eyes included in the study, including 27 eyes with a XEN GelStent implanted and 20 without it. The eyes of people with monocular implementation were the reference group. The values of analysed parameters were compared before and after 10, 25, 40, and 55 min after drinking the water. The intraocular pressure increased by 2.4 mmHg (p < 0.05) for eyes with a XEN stent and 2.2 mmHg for eyes without a stent (p < 0.05) in the tenth minute after drinking of water. This change caused a decreasing of corneal hysteresis (p < 0.05) without significant changes in the corneal resistance factor (p > 0.05). Corneal hysteresis changed similarly in the reference group and the group with a XEN GelStent. The analysis of additional parameters showed a difference in the behaviour of the cornea in eyes with a XEN GelStent in comparison to the corneas of eyes without a stent. This was particularly visible in the analysis of the cornea’s behaviour during the second applanation, when the cornea returns to its baseline state after deformation caused by air puff tonometry.
Comparison of Efficacy and Safety of Brigatinib in First-Line Treatments for Patients with Anaplastic Lymphoma Kinase-Positive Non-Small-Cell Lung Cancer: A Systematic Review and Indirect Treatment Comparison
Authors: Yu Yongfeng , Zhu Fanfan , Zhang Wenxin , Lu Shun
Published: May 2022

(1) Background: The relative efficacy and safety of brigatinib compared with other next-generation anaplastic lymphoma kinase (ALK) inhibitors remains unclear, as first-line head-to-head trials have not been conducted. (2) Methods: Electronic databases were systematically searched for eligible randomized controlled trials (RCT) from January 2010 to October 2021. Outcomes evaluated by indirect treatment comparison (ITC) included progression-free survival (PFS), overall survival (OS), objective response rate (ORR), and safety. (3) Results: Nine RCTs with 2484 patients assessing crizotinib, ceritinib, alectinib, brigatinib, ensartinib, and lorlatinib were included. In intent-to-treat (ITT) patients, brigatinib significantly prolonged blinded independent review committee-assessed PFS compared with crizotinib (HR: 0.48, 95% CI: 0.35 to 0.66) and ceritinib (HR: 0.38, 95% CI: 0.23, 0.60) and had a comparable PFS with other 2nd-generation ALK inhibitors. Subgroup analyses of patients with baseline brain metastases and Asian patients yielded results similar to the base case. Brigatinib significantly reduced the risk of death compared with crizotinib (HR: 0.50, 95% CI: 0.28, 0.87) after adjusting for treatment crossover in the crizotinib arm. No significant differences were observed in OS between brigatinib and other next-generation ALK inhibitors. Brigatinib had significantly superior effects in ORR and intracranial ORR compared to crizotinib. The incidence of grade ≥3 AEs was similar between brigatinib and other next-generation ALK inhibitors (except for alectinib), while brigatinib could significantly delay the time to worsening in the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire-Core 30 (EORTC QLQ-C30) global health status (GHS)/quality of life (QoL) vs. crizotinib (HR: 0.69, 95% CI: 0.49, 0.98). (4) Conclusions: Brigatinib had longer PFS compared to crizotinib and ceritinib and had comparable efficacy and safety profile with other 2nd-generation ALK inhibitors in first-line treatments for patients with ALK-positive non-small-cell lung cancer.
Assessment of the Predictive Value of Spectrophotometric Skin Color Parameters and Environmental and Behavioral Factors in Estimating the Risk of Skin Cancer: A Case–Control Study
Authors: Fijałkowska Marta , Koziej Mateusz , Żądzińska Elżbieta , Antoszewski Bogusław , Sitek Aneta
Published: May 2022

Keratinocyte carcinomas are the most common cancers with different etiological risk factors. The aim of this study was to assess the predictive value of spectrophotometric parameters of skin color in correlation with environmental/behavioral factors to estimate the risk of skin cancer. The case–control study involved 389 patients. The analysis was performed on the training group to build a predictive model and on the testing group to check the quality of the designed model. Area under the curve based on the spectrophotometric skin parameters varied from 0.536 to 0.674. A statistically significant improvement of the area under curve was achieved by adding the number of sunburns for some models. The best single spectrophotometric measurement for estimating skin cancer is the skin melanin index measured on the arm or buttock. Spectrophotometric skin parameters are not very strong but are essential elements of models for estimating the risk of skin cancer. The most important environmental/behavioral factor seems to be the number of sunburns, but not the total exposure to ultraviolet radiation or usage of photoprotectors. Some other pigmentation predictors should be taken into account when creating new models, especially those that can be easily measured in objective and repeatable way. Spectrophotometric measurements can be employed as quick screening skin examination method.
Effects of Coffee on Sirtuin-1, Homocysteine, and Cholesterol of Healthy Adults: Does the Coffee Powder Matter?
Authors: Gonçalinho Gustavo Henrique Ferreira , Nascimento José Rafael de Oliveira , Mioto Bruno Mahler , Amato Reynaldo Vicente , Moretti Miguel Antonio , Strunz Célia Maria Cassaro , César Luiz Antonio Machado , Mansur Antonio de Padua
Published: May 2022

Background: Coffee is one of the most popular beverages globally and contains several bioactive compounds that are relevant to human health. Many nutritional strategies modulate sirtuin-1, thereby impacting aging and cardiometabolic health. This study investigated the influence of different blended coffees on serum sirtuin-1, blood lipids, and plasma homocysteine. Methods: An eight-week randomized clinical trial that included 53 healthy adults of both sexes analyzed the effects of daily intake of 450 to 600 mL of pure Arabica or blended (Arabica + Robusta) coffee intake of filtered coffee on blood sirtuin-1, lipids, and homocysteine. Results: Both Arabica and blended coffees similarly increased serum sirtuin-1 concentration, from 0.51 to 0.58 ng/mL (p = 0.004) and from 0.40 to 0.49 ng/mL (p = 0.003), respectively, without changing plasma homocysteine, folic acid, glucose, and CRP. However, the blended coffee intake increased total cholesterol from 4.70 to 5.17 mmol/L (p < 0.001) and LDL-cholesterol from 2.98 to 3.32 mmol/L (p < 0.001), as well as HDL-c from 1.26 to 1.36 mmol/L (p < 0.001). Conclusion: Both coffee powders increased sirtuin-1 expression, but our results suggest that blended coffee had hypercholesterolemic effects which could increase cardiovascular risk. Therefore, preference should be given to Arabica coffee for the best cardiometabolic benefits of coffee.
Liver Resection for Primary Hepatic Angiosarcoma: Bicentric Analysis of a Challenging Entity
Authors: Katou Shadi , Di Pietro Martinelli Claudine , Silveira Carolina , Schmid Franziska , Becker Felix , Radunz Sonia , Juratli Mazen , Morgul Haluk , Banz Vanessa , Pascher Andreas , Andreou Andreas , Struecker Benjamin
Published: May 2022

Primary hepatic angiosarcoma (PHA) is a rare malignant tumor of the liver, and data on patient outcome after surgical treatment are scarce. The aim of this study was to evaluate postoperative morbidity and overall survival (OS) of patients who underwent hepatectomy for PHA. This is a bicentric retrospective analysis of all consecutive patients who underwent liver resection in curative intent for PHA between 2012 and 2019 at the University Hospital of Muenster and the University Hospital of Bern. Nine patients (five female, four male) were included from both centers. Median age was 72 years (44–82). Most lesions (77.8%) were larger than 5 cm, and mean size of the biggest lesion was 9.4 ± 4.5 cm. Major hepatectomy was performed in four (44.4%), and radical resection (R0) was achieved in six (66.7%) patients. Postoperative complication rate was 88.8%, including 44.4% higher than 3a in the Clavien–Dindo classification. OS survival rates at 1, 2, and 3 years were 44.4%, 22.2%, and 12.5%, respectively, and median OS was 5 months. OS was significantly better after radical resection (R0: 15 months vs. R1: 0 months, p = 0.04), whereas presentation with tumor rupture at diagnosis was associated with the worst OS (0 months vs. 15 months, p = 0.005). Disease recurrence occurred in three patients (33.3%) between three and seven months after surgery. Radical resection remains the only potentially curative treatment option for PHA. However, postoperative morbidity is high, and the overall prognosis remains poor. Multimodal therapy options and management strategies are urgently needed and could improve the prognosis of patients suffering from PHA in the future.
Evaluation of the Wechsler Individual Achievement Test-Fourth Edition as a Measurement Instrument
Authors: Beaujean A. Alexander , Parkin Jason R.
Published: May 2022

The Wechsler Individual Achievement Test (WIAT-4) is the latest iteration of a popular instrument that psychologists employ to assess academic achievement. The WIAT-4 authors make both pragmatic and measurement claims about the instrument. The pragmatic claims involve being useful for identifying individuals in certain academic achievement-related groups (e.g., specific learning disability). The measurement claims are twofold: (a) the instrument’s scores represent psychological attributes, and (b) scores transformed to standard score values have equal-interval properties. The WIAT-4 authors did not provide the evidence necessary to support the pragmatic claims in the technical manual, so we could not evaluate them. Thus, we limited our evaluation to the measurement claims for the composite scores. To do so, we used information in the technical manual along with some additional factor analyses. Support for the first measurement claim varies substantially across scores. Although none of the evidence is particularly strong, scores in mathematics and reading domains tend to have more support than the writing and total achievement scores. Support for the second claim was insufficient for all scores. Consequently, we recommend that psychologists wishing to interpret WIAT-4 composite scores limit those interpretations to just a few in the mathematics and reading domains. Second, psychologists should completely refrain from using any composite score in a way that requires equal-interval values (e.g., quantitative score comparisons). Neither of these recommendations necessarily disqualifies the scores from being useful for pragmatic purposes, but support for these uses will need to come from evidence not currently provided in the WIAT-4 technical manual.
Investigating the Influence of Material Extrusion Rates and Line Widths on FFF-Printed Graphene-Enhanced PLA
Authors: Butt Javaid , Bhaskar Raghunath , Mohaghegh Vahaj
Published: May 2022

Fused filament fabrication (FFF) is a widely used additive manufacturing process that can produce parts from thermoplastics. Its ease of operation and wide variety of materials make it a popular choice for manufacturing. To leverage such benefits, the commonly used thermoplastics (e.g., PLA and ABS) are impregnated with nanoparticles, short or continuous fibers, and other additives. The addition of graphene nanoplatelets to PLA makes for a high-quality filament possessing enhanced mechanical, electrical, and thermal properties. Even with the advancement in materials, the optimisation of the process parameter remains the most complex aspect for FFF. Therefore, this study investigates the influence of two under-researched and overlooked processing parameters (material extrusion rates and line widths) on commercially available graphene-enhanced PLA (GPLA). Nine different material extrusion rates (70% to 150%) and five different line widths (0.2 mm to 1 mm) were used to manufacture GPLA specimens using a low-cost, desktop-based 3D printer, as per British and international standards. The study analyses the influence of these two processing parameters on mass, dimensional accuracy, surface texture, and mechanical properties of GPLA specimens. A non-destructive test has also been conducted and correlated with three-point flexural test to establish its applicability in evaluating flexural properties of GPLA. The results how that small line widths provide more accuracy with longer print times whereas large line widths offer more strength with shorter printing times. Increase in material extrusion rates adversely affect the surface finish and hardness but positively influence the flexural strength of GPLA specimens. The study shows that the manipulation of material extrusion rates and line widths can help designers in understanding the limitations of the default printing settings (100% material extrusion rate and 0.4 mm line width) on most desktop 3D printers and identifying the optimal combination to achieve desired properties using the FFF process.
Numerical Simulation of Vortex-Induced Vibration of TTR and SCR
Authors: Wang Jiachen , He Zhilong , Li Dantong , Wu Weifeng
Published: May 2022

Top tensioned risers (TTRs) and steel catenary risers (SCRs) have been widely used in the field of marine engineering. They are commonly used to transport fluids from subsea wells to surface platforms. Under the action of ocean currents, these risers are often subjected to vortex-induced vibrations (VIVs), which might lead to serious fatigue damage. In this study, VIV around TTR and SCR were numerically simulated using the computational fluid dynamics software FLUENT when the Reynolds number was 4000. In the calculations, the full hexahedron grid and large eddy simulation were used to ensure calculation accuracy from the boundary conditions, as well as solution control. The shape, frequency, and amplitude of VIV produced by TTRs and SCRs at different times and depths were simulated.
Identification of Genomic Variants Associated with the Risk of Acute Lymphoblastic Leukemia in Native Americans from Brazilian Amazonia
Authors: Leitão Luciana P. C. , de Carvalho Darlen C. , Rodrigues Juliana C. G. , Fernandes Marianne R. , Wanderley Alayde V. , Vinagre Lui W. M. S. , da Silva Natasha M. , Pastana Lucas F. , Gellen Laura P. A. , Assunção Matheus C. E. , Fernandes Sweny S. M. , Pereira Esdras E. B. , Ribeiro-Dos-Santos André M. , Guerreiro João F. , Ribeiro-dos-Santos Ândrea , de Assumpção Paulo P. , dos Santos Sidney E. B. , dos Santos Ney P. C.
Published: May 2022

A number of genomic variants related to native American ancestry may be associated with an increased risk of developing Acute Lymphoblastic Leukemia (ALL), which means that Latin American and hispanic populations from the New World may be relatively susceptible to this disease. However, there has not yet been any comprehensive investigation of the variants associated with susceptibility to ALL in traditional Amerindian populations from Brazilian Amazonia. We investigated the exomes of the 18 principal genes associated with susceptibility to ALL in samples of 64 Amerindians from this region, including cancer-free individuals and patients with ALL. We compared the findings with the data on populations representing five continents available in the 1000 Genomes database. The variation in the allele frequencies found between the different groups was evaluated using Fisher’s exact test. The analyses of the exomes of the Brazilian Amerindians identified 125 variants, seven of which were new. The comparison of the allele frequencies between the two Amerindian groups analyzed in the present study (ALL patients vs. cancer-free individuals) identified six variants (rs11515, rs2765997, rs1053454, rs8068981, rs3764342, and rs2304465) that may be associated with susceptibility to ALL. These findings contribute to the identification of genetic variants that represent a potential risk for ALL in Amazonian Amerindian populations and might favor precision oncology measures.
Inflammation and Oxidative Stress in Seminal Plasma: Search for Biomarkers in Diagnostic Approach to Male Infertility
Authors: Bruno Carmine , Basile Umberto , Vergani Edoardo , Napodano Cecilia , Oliva Alessandro , Gulli Francesca , Meucci Elisabetta , Silvestrini Andrea , Orlando Patrick , Silvestri Sonia , Tiano Luca , Mancini Antonio
Published: May 2022

Oxidative and inflammatory damage underlie several conditions related to male infertility, including varicocele. Free light chains of immunoglobulins (FLCs) are considered markers of low-grade inflammation in numerous diseases. Coenzyme Q10 (CoQ10), a lipidic antioxidant and anti-inflammatory compound, is involved in spermatozoa energy metabolism and motility. We aimed to evaluate FLCs’ seminal levels in patients with varicocele in comparison to control subjects and to correlate them with CoQ10 and Total Antioxidant Capacity (TAC) in human semen. Sixty-five patients were enrolled. Semen analysis was performed; patients were divided into three groups: controls, 12 normozoospermic patients, aged 34 (33–41) years; varicocele (VAR), 29 patients, aged 33 (26–37) years; and idiopathic, 24 oligo-, astheno- and oligoasthenozoospermic patients aged 37 (33.5–40.5) years. FLCs (κ and λ) were assayed by turbidimetric method; CoQ10 by HPLC; TAC by spectrophotometric method. λ FLCs showed a trend toward higher levels in VAR vs. controls and the idiopathic group. VAR showed a trend toward lower κ FLCs levels vs. the other two groups. When comparing κ/λ ratio, VAR showed significantly lower levels vs. controls and idiopathic. Moreover, CoQ10 seminal levels showed higher levels in VAR and idiopathic compared to controls. Data reported here confirm lower levels of κ/λ ratio in VAR and suggest a possible application in personalized medicine as clinical biomarkers for male infertility.
Endocrine and Metabolic Illnesses in Young Adults with Prader–Willi Syndrome
Authors: Noh Eu-Seon , Kim Min-Sun , Kim Chiwoo , Jeon Kyeongman , Kim Seonwoo , Cho Sung Yoon , Jin Dong-Kyu
Published: May 2022

Prader–Willi syndrome (PWS) is a rare genetic disorder characterized by an insatiable appetite that leads to morbid obesity. Previous studies reported health problems in adults with PWS. However, studies on younger adults are lacking, and there are no specific studies of endocrine and metabolic illness in this age group. We performed a retrospective cohort study of 68 individuals with PWS aged 19 to 34 years at Samsung Medical Center. The prevalence of endocrine and metabolic illnesses were compared with those in an age-, sex-, and BMI-matched healthy control group. Young adults with PWS had a higher prevalence of metabolic syndrome (35.3% vs. 4.4%), type 2 diabetes mellitus (50.0% vs. 5.4%), hypertension (30.8% vs. 16.1%), dyslipidemia (38.2% vs. 14.7%), decreased bone density (26.4% vs. 0.9%), and sleep apnea (32.3% vs. 4.4%) than controls (all p < 0.05). The PWS group that maintained recombinant human growth (rhGH) treatment in adulthood had a lower probability of having a BMI ≥ 30 at the last follow-up (odds ratio = 0.106 (0.012–0.948), p = 0.045). Endocrine and metabolic illnesses in individuals with PWS may have already started in the early teens; therefore, appropriate screening and early intervention are important. Better understanding of the natural history of PWS and age-related complications will lead to better-quality medical care for individuals with PWS.