bluuebunny/crossref_metadata_2025 huggingface dataset:
Characteristics of photosynthesis and vertical canopy architecture of citrus trees under two labor-saving cultivation modes using unmanned aerial vehicle (UAV)-based LiDAR data in citrus orchards
Authors: Dian Yuanyong , Liu Xiaoyang , Hu Lei , Zhang Jinzhi , Hu Chungen , Liu Yongzhong , Zhang Jinxin , Zhang Wenbo , Hu Qingqing , Zhang Yahao , Fang Yanni , Zhou Jingjing
Published: February 2023

Analyzing and comparing the effects of labor-saving cultivation modes on photosynthesis, as well as studying their vertical canopy architecture, can improve the tree structure of high-quality and high-yield citrus and selection of labor-saving cultivation modes. The photosynthesis of 1080 leaves of two labor-saving cultivation modes (wide-row and narrow-plant mode and fenced mode) comparing with the traditional mode were measured, and nitrogen content of all leaves and photosynthetic nitrogen use efficiency (PNUE) were determined. Unmanned aerial vehicle (UAV)-based light detection and ranging (LiDAR) data were used to assess the vertical architecture of three citrus cultivation modes. Results showed that for the wide-row and narrow-plant and traditional modes leaf photosynthetic CO2 assimilation rate, stomatal conductance, and transpiration rate of the upper layer were significantly higher than those of the middle layer, and values of the middle layer were markedly higher than those of the lower layer. In the fenced mode, a significant difference in photosynthetic factors between the upper and middle layers was not observed. A vertical canopy distribution had a more significant effect on PNUE in the traditional mode. Leaves in the fenced mode had distinct photosynthetic advantages and higher PNUE. UAV-based LiDAR data effectively revealed the differences in the vertical canopy architecture of citrus trees by enabling calculating the density and height percentile of the LiDAR point cloud. The point cloud densities of three cultivation modes were significantly different for all LiDAR density slices, especially at higher canopy heights. The labor-saving modes, particularly the fenced mode, had significantly higher height percentile data.
A functioning Björk–Shiley aortic valve after 36 years without anticoagulation
Authors: Helms Florian , Rosebrock Frauke , Haverich Axel , Rustum Saad
Published: February 2023

There is a broad consensus among all clinical guidelines that lifelong oral anticoagulation is mandatory after mechanical valve prosthesis implantation. However, in rare cases, patients do not receive anticoagulation or anticoagulation therapy is withdrawn over time. We present a case of an exceptionally durable Björk–Shiley mechanical aortic valve prosthesis still functioning 49 years after implantation. Remarkably, the patient did not receive any anticoagulation or antiplatelet therapy for the first 36 years after implantation. Despite this, no thromboembolic or valve-related adverse events occurred to date. Upon thorough echocardiographic assessment, excellent valve function with a mean transvalvular gradient of 13 mmHg and no prosthetic valve insufficiency was found. This makes the case presented here one of the longest functioning mechanical valve replacements reported.
Lower bounds, elliptic reconstruction and a posteriori error control of parabolic problems
Authors: Georgoulis Emmanuil H , Makridakis Charalambos G
Published: February 2023

A popular approach for proving a posteriori error bounds in various norms for evolution problems with partial differential equations uses reconstruction operators to recover conforming objects from the approximate solutions. So far, lower bounds in reconstruction-based a posteriori error estimators have been proven only for time-discrete schemes for parabolic problems; the proof of lower bounds for fully discrete schemes in reconstruction-based a posteriori error estimators has eluded. In this work, we provide a complete framework addressing this issue for energy-type norms. We consider Backward Euler discretizations and time-discontinuous Galerkin schemes, combined with dynamically changing conforming finite element methods in space, approximating linear parabolic problems. The results presented include sharp upper and lower a posteriori error bounds. Localized versions of the lower bounds are also considered.
Allostatic load and cardiovascular outcomes in males with prostate cancer
Authors: Stabellini Nickolas , Cullen Jennifer , Bittencourt Marcio S , Moore Justin X , Cao Lifen , Weintraub Neal L , Harris Ryan A , Wang Xiaoling , Datta Biplab , Coughlin Steven S , Garcia Jorge , Shanahan John , Hamerschlak Nelson , Waite Kristin , Fillmore Nathanael R , Terris Martha , Montero Alberto J , Barnholtz-Sloan Jill S , Guha Avirup
Published: February 2023

Background

Cardiovascular disease (CVD) is the leading cause of death in men with prostate cancer (PC). Accumulated stress plays an important role in CVD development. The cumulative burden of chronic stress and life events can be measured using allostatic load (AL).

Methods

The initial cohort included males aged 18 years and older diagnosed with PC (2005-2019). AL was modeled as an ordinal variable (0-11). Fine-Gray competing risk regressions measured the impact of precancer diagnosis AL and postdiagnosis AL in 2-year major cardiac events (MACE). The effect of AL changes over time on MACE development was calculated via piecewise Cox regression (before, and 2 months, 6 months, and 1 year after PC diagnosis).

Results

We included 5261 PC patients of which 6.6% had a 2-year MACE. For every 1-point increase in AL before and within 60 days after PC diagnosis, the risk of MACE increased 25% (adjusted hazard ratio [aHR] =1.25, 95% confidence interval [CI] = 1.18 to 1.33) and 27% (aHR = 1.27, 95% CI = 1.20 to 1.35), respectively. Using AL as a time-varying exposure, the risk of MACE increased 19% (aHR = 1.19, 95% CI = 1.11 to 1.27), 22% (aHR = 1.22, 95% CI = 1.14 to 1.33), 28% (aHR = 1.28, 95% CI = 1.23 to 1.33), and 31% (aHR = 1.31, 95% CI = 1.27 to 1.35) for every 1-point increase in AL before, 2 months after, 6 months after, and 1 year after PC diagnosis, respectively.

Conclusion

AL and its changes over time are associated with MACE in PC patients, suggesting a role of a biological measure of stress as a marker of CVD risk among men with PC.
System Structure, Unjust War, and State Excusability
Authors: Rubin David
Published: February 2023

This article seeks to bridge the interdisciplinary gap between just war theory (JWT) and international relations (IR) by introducing into the former discipline key theoretical and empirical insights from the latter discipline. Specifically, the article argues that traditional JWT is deficient as a normative account of war because it constitutes what Kenneth Waltz calls a “unit-level” theory, operating at the level of the individual state and evaluating the morality of a given war solely by reference to a list of state-level factors (e.g., just cause, proportionality, etc.). In this manner, JWT fails to take account of the systemic, or structural, correlates of international armed conflict. In particular, it fails to incorporate insights from the leading mainstream IR theories of neorealism, institutionalism, and constructivism in regard to how the international distribution of material capabilities, institutions, and ideas codetermines the nature and likelihood of war. To remedy these inadequacies of JWT, a “multilevel” approach to the morality of war is put forward according to which unit-level factors are required to be weighted by systemic factors. Pursuant to this approach, if a state has initiated or participated in a war that is assessed as unjust through the unit-level lens of traditional JWT, the state itself can still sometimes be partially excused for that war once systemic factors have been considered.
Palynomorphological Observations on the GenusSabulinaRchb. (Caryophyllaceae) from Turkey Using Light Microscopy and Scanning Electron Microscopy
Authors: Özbek Funda
Published: February 2023

Pollen morphology of 28 taxa belonging to the genus Sabulina Rchb. (Caryophyllaceae) from Turkey was examined and compared using light microscopy and scanning electron microscopy. The current study aimed to describe the palynological characteristics of the Sabulina taxa and to assess the diagnostic value of these features for taxonomic implications. The pollen grains of Sabulina are radially symmetrical, apolar, rarely isopolar, and pantopolyporate with 8–28 pores. Their shape is prolate-spheroidal with the A axes (long axes) 19.5 μm and the B axes (short axes) 19–39.5 μm. Exine sculpturing is microechinate-punctate in all taxa. Principal component analysis was used to evaluate the significant pollen morphological characters for distinguishing the taxa, with three components accounting for 95.112% of the total variance. Numerical analysis revealed that pollen sizes, pore diameters, pore numbers, distance between pores, spinule density, and punctum numbers are particularly the most useful variables for separating the Sabulina taxa. The results also display a partial relationship between the Sabulina taxa clusters based on pollen morphology and the systematics of these taxa based on general macromorphology.
Far infrared treatment on the arteriovenous fistula induces changes in sVCAM and sICAM in patients on hemodialysis
Authors: Lindhard Kristine , Jensen Boyle L , Pedersen Brian L , Meyer-Olesen Christine , Rix Marianne , Hansen Henrik P , Schalkwijk Casper , Waarenburg Marjo , Heaf James , Hansen Ditte
Published: February 2023

Introduction

There is a substantial risk of developing stenosis and dysfunction in the arteriovenous fistula (AVF) in patients on hemodialysis (HD). Far infrared radiation (FIR) is a non-invasive local intervention with a potentially beneficial effect on AVF patency. The underlying mechanism is not clear. It was hypothesized that a single FIR treatment reduces factors of inflammation and promotes endothelial vasodilators in the AVF.

Methods

Forty HD patients with an AVF were included in an open-label intervention study. Patients were randomized to receive either FIR (FIR group) or no FIR (control group). Blood samples were drawn directly from the AVF and from a peripheral vein in the non-AVF arm before (T0) and 40 min after (T40) treatment during a HD session. The changes [median (interquartile range)] in circulating factors of inflammation, endothelial function and vasoreactivity during FIR were measured.

Results

In the AVF a single FIR treatment during dialysis resulted in a significantly diminished decrease in soluble vascular cell adhesion molecule, sVCAM [−31.6 (−54.3; 22.1) vs −89.9 (−121.6; −29.3), P = .005] and soluble intercellular adhesion molecule, sICAM [−24.2 (−43.5; 25.3) vs −49 (−79.9; −11.6), P = .02] compared with the control group. Other factors, such as interleukins, nitrite, nitrate and tumor necrosis factor 1, also declined during dialysis, but with no significant differences related to FIR in either the AVF or the non-AVF arm.

Conclusion

A single FIR treatment attenuated the decrease in sVCAM and sICAM in the AVF compared with a control group during HD. Findings do not support the hypothesis of a vaso-protective effect of FIR. The long-term effects of FIR on the AVF are unknown.
Soil and vegetation water content identify the main terrestrial ecosystem changes
Authors: Bueso Diego , Piles Maria , Ciais Philippe , Wigneron Jean-Pierre , Moreno-Martínez Álvaro , Camps-Valls Gustau
Published: February 2023

Environmental change is a consequence of many interrelated factors. How vegetation responds to natural and human activity still needs to be well established, quantified and understood. Recent satellite missions providing hydrologic and ecological indicators enable better monitoring of Earth system changes, yet there is no automatic way to address this issue directly from observations. Here, we develop an observation-based methodology to capture evidence of changes in global terrestrial ecosystems and attribute these changes to natural or anthropogenic activity. We use the longest time record of global microwave L-band soil moisture and vegetation optical depth as satellite data and build spatially explicit maps of change in soil and vegetation water content and biomass reflecting large ecosystem changes during the last decade, 2010–20. Regions of prominent trends (from $-8\%$ to 9% per year) are observed, especially in humid and semi-arid climates. We further combine such trends with land cover change maps, vegetation greenness and precipitation variability to assess their relationship with major documented ecosystem changes. Several regions emerge from our results. They cluster changes according to human activity drivers, including deforestation (Amazon, Central Africa) and wildfires (East Australia), artificial reforestation (South-East China), abandonment of farm fields (Central Russia) and climate shifts related to changes in precipitation variability (East Africa, North America and Central Argentina). Using the high sensitivity of soil and vegetation water content to ecosystem changes, microwave satellite observations enable us to quantify and attribute global vegetation responses to climate or anthropogenic activities as a direct measure of environmental changes and the mechanisms driving them.
Regional Variation in Outpatient Antibiotic Prescribing for Acute Respiratory Tract Infections in a Commercially Insured Population, United States, 2017
Authors: Bizune Destani , Tsay Sharon , Palms Danielle , King Laura , Bartoces Monina , Link-Gelles Ruth , Fleming-Dutra Katherine , Hicks Lauri A
Published: February 2023

Background

Studies have shown that the Southern United States has higher rates of outpatient antibiotic prescribing rates compared with other regions in the country, but the reasons for this variation are unclear. We aimed to determine whether the regional variability in outpatient antibiotic prescribing for respiratory diagnoses can be explained by differences in prescriber clinical factors found in a commercially insured population.

Methods

We analyzed the 2017 IBM MarketScan Commercial Database of commercially insured individuals aged <65 years. We included visits with acute respiratory tract infection (ARTI) diagnoses from retail clinics, urgent care centers, emergency departments, and physician offices. ARTI diagnoses were categorized based on antibiotic indication. We calculated risk ratios and 95% CIs stratified by ARTI tier and region using log-binomial models controlling for patient age, comorbidities, care setting, prescriber type, and diagnosis.

Results

Of the 14.9 million ARTI visits, 40% received an antibiotic. The South had the highest proportion of visits with an antibiotic prescription (43%), and the West the lowest (34%). ARTI visits in the South are 34% more likely receive an antibiotic for rarely antibiotic-appropriate ARTI visits when compared with the West in multivariable modeling (relative risk, 1.34; 95% CI, 1.33–1.34).

Conclusions

It is likely that higher antibiotic prescribing in the South is in part due to nonclinical factors such as regional differences in clinicians' prescribing habits and patient expectations. There is a need for future studies to define and characterize these factors to better inform regional and local stewardship interventions and achieve greater health equity in antibiotic prescribing.
Nationwide Measles and Rubella Outbreaks in South Sudan, 2019
Authors: Peck Megan E , Maleghemi Sylvester , Kayembe Lidia , Hercules Margaret , Anyuon Atem , Bunga Sudhir , McFarland Jeff , Olu Olushayo
Published: February 2023

Background

South Sudan confirmed a measles outbreak in December 2018. An investigation was conducted to assess underlying causes of the outbreak.

Methods

Vaccination coverage and measles surveillance data were analyzed. A suspected measles case had fever, maculopapular rash, and cough or conjunctivitis. A confirmed measles case had generalized maculopapular rash lasting >3 days, a temperature >38°C, and cough or conjunctivitis; or serologic confirmation (anti-measles immunoglobin M [IgM] antibody detection) in serum samples collected ≤30 days from rash onset. A confirmed rubella case tested measles IgM-negative and rubella IgM-positive.

Results

Nationwide, 3727 suspected measles cases were reported in 2019. Seventy-five percent of all suspected measles cases were in children aged <5 years. Thirty-six percent of patients with suspected measles were admitted to the hospital, and 36 measles-related deaths were reported. Among cases, 922 (25%) were tested for measles; of these, 317 (34%) were measles IgM-positive. Among cases that tested measles IgM-negative, 149 (33%) were rubella IgM-positive. Immunization coverage for 1 dose of measles-containing vaccine (MCV) varied by state, ranging from 6% to 67%.

Conclusions

Measles and rubella remain public health problems in South Sudan. To reduce measles incidence, South Sudan needs to achieve >95% coverage with 2 doses of MCV.
InvasiveScedosporiumandLomentospora prolificansInfections in Australia: A Multicenter Retrospective Cohort Study
Authors: Neoh Chin Fen , Chen Sharon C A , Crowe Amy , Hamilton Kate , Nguyen Quoc A , Marriott Debbie , Trubiano Jason A , Spelman Tim , Kong David C M , Slavin Monica A
Published: February 2023

Background

Management of Scedosporium/Lomentospora prolificans infections remains challenging. We described predisposing factors, clinical manifestations, and outcomes of these rare mold infections, including predictors of early (1-month) and late (18-month) all-cause mortality and treatment failure.

Methods

We conducted a retrospective Australian-based observational study of proven/probable Scedosporium/L prolificans infections from 2005 to 2021. Data on patient comorbidities, predisposing factors, clinical manifestations, treatment, and outcomes up to 18 months were collected. Treatment responses and death causality were adjudicated. Subgroup analyses, multivariable Cox regression, and logistic regression were performed.

Results

Of 61 infection episodes, 37 (60.7%) were attributable to L prolificans. Forty-five of 61 (73.8%) were proven invasive fungal diseases (IFDs), and 29 of 61 (47.5%) were disseminated. Prolonged neutropenia and receipt of immunosuppressant agents were documented in 27 of 61 (44.3%) and 49 of 61 (80.3%) episodes, respectively. Voriconazole/terbinafine was administered in 30 of 31 (96.8%) L prolificans infections, and voriconazole alone was prescribed for 15 of 24 (62.5%) Scedosporium spp infections. Adjunctive surgery was performed in 27 of 61 (44.3%) episodes. Median time to death post–IFD diagnosis was 9.0 days, and only 22 of 61 (36.1%) attained treatment success at 18 months. Those who survived beyond 28 days of antifungal therapy were less immunosuppressed with fewer disseminated infections (both P < .001). Disseminated infection and hematopoietic stem cell transplant were associated with increased early and late mortality rates. Adjunctive surgery was associated with lower early and late mortality rates by 84.0% and 72.0%, respectively, and decreased odds of 1-month treatment failure by 87.0%.

Conclusions

Outcomes associated with Scedosporium/L prolificans infections is poor, particularly with L prolificans infections or in the highly immunosuppressed population.
Predictors of Human Immunodeficiency Virus Pre-Exposure Prophylaxis (PrEP) Uptake in a Sexual Health Clinic With Rapid PrEP Initiation
Authors: Wagner Gabriel A , Wu Kuan-Sheng , Anderson Christy , Burgi Alina , Little Susan J
Published: February 2023

Background

Improved pre-exposure prophylaxis (PrEP) uptake is essential for human immunodeficiency virus (HIV) prevention initiatives. Offering PrEP at the time of HIV and sexually transmitted infection (STI) testing can improve uptake. We offered rapid PrEP initiation in a sexual health clinic and assessed predictors of PrEP interest, initiation, linkage, and retention.

Methods

Between November 2018 and February 2020, PrEP-eligible individuals who presented to a sexual health clinic were offered a free 30-day supply of PrEP plus linkage to continued PrEP care. Univariable and multivariable analyses of demographic and HIV risk data were conducted to determine predictors of PrEP uptake.

Results

Of 1259 adults who were eligible for PrEP (99.7% male, 42.7% White, 36.2% Hispanic), 456 were interested in PrEP, 249 initiated PrEP, 209 were linked, and 67 were retained in care. Predictors of PrEP interest included younger age (P < .01), lower monthly income (P = .01), recreational drug use (P = .02), and a greater number of sexual partners (P < .01). Negative predictors of PrEP initiation included lower monthly income (P = .04), testing positive for chlamydia (P = .04), and exchanging money for sex (P = .01). Negative predictors of linkage included self-identifying as Black (P = .03) and testing positive for an STI (P < .01). Having health insurance positively predicted both linkage (P < .01) and retention (P < .03).

Conclusions

A minority of PrEP-eligible HIV and STI testers initiated PrEP when offered, suggesting that easy PrEP access in sexual health clinics alone may not improve uptake. Predictors of uptake included established HIV risk factors and markers of higher socioeconomic status, suggesting that those aware of their risk and with the means to utilize health services engaged best with this model.
Investigating Epidemiologic and Molecular Links Between Patients With Community- and Hospital-Acquired Influenza A: 2017–2018 and 2019–2020, Michigan
Authors: Wan Tiffany , Lauring Adam S , Valesano Andrew L , Fitzsimmons William J , Bendall Emily E , Kaye Keith S , Petrie Joshua G
Published: February 2023

Background

Hospital-acquired influenza virus infection (HAII) can cause severe morbidity and mortality. Identifying potential transmission routes can inform prevention strategies.

Methods

We identified all hospitalized patients testing positive for influenza A virus at a large, tertiary care hospital during the 2017–2018 and 2019–2020 influenza seasons. Hospital admission dates, locations of inpatient service, and clinical influenza testing information were retrieved from the electronic medical record. Time-location groups of epidemiologically linked influenza patients were defined and contained ≥1 presumed HAII case (first positive ≥48 hours after admission). Genetic relatedness within time-location groups was assessed by whole genome sequencing.

Results

During the 2017–2018 season, 230 patients tested positive for influenza A(H3N2) or unsubtyped influenza A including 26 HAIIs. There were 159 influenza A(H1N1)pdm09 or unsubtyped influenza A–positive patients identified during the 2019–2020 season including 33 HAIIs. Consensus sequences were obtained for 177 (77%) and 57 (36%) of influenza A cases in 2017–2018 and 2019–2020, respectively. Among all influenza A cases, there were 10 time-location groups identified in 2017–2018 and 13 in 2019–2020; 19 of 23 groups included ≤4 patients. In 2017–2018, 6 of 10 groups had ≥2 patients with sequence data, including ≥1 HAII case. Two of 13 groups met this criteria in 2019–2020. Two time-location groups from 2017–2018 each contained 3 genetically linked cases.

Conclusions

Our results suggest that HAIIs arise from outbreak transmission from nosocomial sources as well as single infections from unique community introductions.
Bictegravir-Induced Drug Reaction With Eosinophilia and Systemic Symptoms in a Patient With Acute Human Immunodeficiency Virus
Authors: DiLorenzo Madeline A , Medrano Nicola , Chen Jason N , Bawany Fatima , Tran Duy C , Taunk Pulkit , Meehan Shane A , Pomeranz Miriam Keltz , Mgbako Ofole
Published: February 2023

Although drug reaction with eosinophilia and systemic symptoms (DRESS) is associated with antiretrovirals, there are no published reports of bictegravir-induced DRESS. Bictegravir is recommended as first-line treatment for patients with human immunodeficiency virus (HIV). Recognition of DRESS, its skin manifestations, and potential complications is vital for appropriate care and management of acute HIV.
Non-AIDS-Defining Events in Human Immunodeficiency Virus Controllers Versus Antiretroviral Therapy–Controlled Patients: A Cohort Collaboration From the French National Agency for Research on AIDS CO21 (CODEX) and CO06 (PRIMO) Cohorts
Authors: Manto Carmelite , Castro-Gordon Alicia , Goujard Cécile , Meyer Laurence , Lambotte Olivier , Essat Asma , Shaiykova Arnoo , Boufassa Faroudy , Noël Nicolas
Published: February 2023

Background

Low-grade chronic inflammation may persist in spontaneous human immunodeficiency virus controllers (HICs), leading to non-AIDS-defining events (nADEs).

Methods

Two hundred twenty-seven antiretroviral therapy (ART)–naive HICs (known human immunodeficiency virus type 1 [HIV-1] infection ≥5 years and at least 5 consecutive viral loads [VLs] <400 HIV RNA copies/mL) were compared with 328 patients who initiated ART ≤1 month after primary HIV infection diagnosis and had undetectable VL within 12 months following ART initiation for at least 5 years. Incidence rates of first nADEs were compared between HICs and ART-treated patients. Determinants of nADEs were assessed by using Cox regression models.

Results

All-cause nADEs incidence rates were 7.8 (95% confidence interval [CI], 5.9–9.6) and 5.2 (95% CI, 3.9–6.4) per 100 person-months among HICs and ART patients, respectively (incidence rate ratio [IRR], 1.5 [95% CI, 1.1–2.2]; adjusted IRR, 1.93 [95% CI, 1.16–3.20]). After adjustment for the cohort, demographic, and immunological characteristics, the only other factor associated with all-cause nADE occurrence was age ≥43 (vs <43) years at the beginning of viral control (IRR, 1.69 [95% CI, 1.11–2.56]). The most frequent events observed in the 2 cohorts were non-AIDS-related benign infections (54.6% and 32.9% of all nADEs, respectively, for HICs and ART patients). No differences in cardiovascular or psychiatric events were observed.

Conclusions

HICs experienced 2 times more nADEs than virologically suppressed patients on ART, mainly non-AIDS-related benign infections. Older age was associated with nADE occurrence, independent of immune or virologic parameters. These results do not argue in favor of expanding the ART indication for HICs but rather a case-by-case approach considering clinical outcomes such as nADEs besides immune activation.
Medieval Magic
Authors: Page Sophie
Published: February 2023

Despite the condemnations uttered by some theologians at the time, there was a flowering of intellectual interest in magic in the medieval period. Natural magic was an aspect of experimental science, and angelic magic was practised as a religious exercise. The Crusades and the mistaken attribution of the ‘witch craze’ as medieval have shaped the popular conception of the period as one of intolerance. This chapter shows, through examples from magical manuscripts, how branches of medieval scholarship were enlightened. Magic was a product of dialogue with and inspiration from Judaic and Arabic traditions that preserved ancient knowledge otherwise lost in the Christian West. The importance of Spain and Byzantium in this process is explored.
Lifespan developmental invariance in memory consolidation: evidence from procedural memory
Authors: Tóth-Fáber Eszter , Nemeth Dezso , Janacsek Karolina
Published: February 2023

Characterizing ontogenetic changes across the lifespan is a crucial tool in understanding neurocognitive functions. While age-related changes in learning and memory functions have been extensively characterized in the past decades, the lifespan trajectory of memory consolidation, a critical function that supports the stabilization and long-term retention of memories, is still poorly understood. Here we focus on this fundamental cognitive function and probe the consolidation of procedural memories that underlie cognitive, motor, and social skills and automatic behaviors. We used a lifespan approach: 255 participants aged between 7 and 76 years performed a well-established procedural memory task in the same experimental design across the whole sample. This task enabled us to disentangle two critical processes in the procedural domain: statistical learning and general skill learning. The former is the ability to extract and learn predictable patterns of the environment, while the latter captures a general speed-up as learning progresses due to improved visuomotor coordination and other cognitive processes, independent of acquisition of the predictable patterns. To measure the consolidation of statistical and general skill knowledge, the task was administered in two sessions with a 24-h delay between them. Here, we report successful retention of statistical knowledge with no differences across age groups. For general skill knowledge, offline improvement was observed over the delay period, and the degree of this improvement was also comparable across the age groups. Overall, our findings reveal age invariance in these two key aspects of procedural memory consolidation across the human lifespan.
The Strategic Use of Corporate Philanthropy: Evidence from Bank Donations
Authors: Choi Seungho , Park Raphael Jonghyeon , Xu Simon
Published: February 2023

This article examines the strategic nature of banks’ charitable giving by studying bank donations to local nonprofit organizations. Relying on the application of antitrust rules in bank mergers as an exogenous shock to local deposit market competition, we find that local competition affects banks’ local donation decisions. Using county-level natural disaster shocks, we show that banks with disaster exposure reallocate donations away from nonshocked counties, where they operate branches, and toward shocked counties. The reallocation of donations represents an exogenous increase in the local share of donations in nonshocked counties for banks with no disaster exposure and leads to an increase in the local deposit market shares of such banks. Furthermore, banks can potentially earn greater profits from making donations and tend to donate to nonprofits that have the most social impact. Overall, our evidence suggests that banks participate in corporate philanthropy strategically to enhance performance.
Host Genetic Traits Underlying the Composition and Assembly of the Citrus Microbiome
Authors: Xu Jin , Wang Yayu , Zhang Yunzeng , Riera Nadia , Li Jinyun , Clark Kelley J. , Jin Tao , Chen Hongyun , Wen Jiawen , Ma Wenbo , Liu Huan , Wang Nian
Published: February 2023

Although plant genotypes are known to be a determinant of microbiome composition, the genetic mechanisms underlying how genotypes affect the microbiome are poorly understood. Citrus is an important, perennial fruit crop that normally contains scion grafted on the rootstock. Here, we investigated the genetic traits underlying the citrus microbiome. We sequenced the metagenome of leaf, root, and rhizosphere soil samples and the genome of corresponding citrus genotypes, which included 66 unique rootstock–scion combinations (56 scion genotypes and 9 rootstock genotypes) from one location using deep shotgun sequencing. Using a genome-wide association study (GWAS) analysis, we identified significant associations between citrus genes and a subset of genera and functional traits of the microbiome. Intriguingly, we found that bacterial secretion systems and mobility-related genes were present in single-nucleotide polymorphism-associated microbial functional traits of leaf and root microbiomes but not in the rhizosphere microbiome, indicating that they are important factors for bacteria to colonize inside or on leaf and root tissues but less so in the rhizosphere. We also discovered that chemotaxis and flagella genes play critical roles for bacteria colonizing leaf tissues but not in root tissues and the rhizosphere. Microbiome-associated host genes were mainly involved in plant immunity, transporters, hormones, cell walls, and the metabolism of carbohydrates, amino acids, and nitrogen. We identified genetic determinants that are associated with the abundance of Bacillus, Bradyrhizobium, Burkholderia, and Cellvibrio, genera that are known to comprise many beneficial bacteria for citrus. This study unraveled the genetic traits underneath the mutual selection of citrus and microbes via GWAS analyses. This study provides useful information that aids the utilization of genotypes and key members of a healthy microbiota for microbiome engineering as well as the editing of specific genes to improve host microbiota.

[Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Neutrophilia in severe asthma is reduced in Ormdl3 overexpressing mice
Authors: James Briana N. , Weigel Cynthia , Green Christopher D. , Brown Ryan D. R. , Palladino Elisa N. D. , Tharakan Anuj , Milstien Sheldon , Proia Richard L. , Martin Rebecca K. , Spiegel Sarah
Published: February 2023

Genome‐wide association studies have linked the ORM (yeast)–like protein isoform 3 (ORMDL3) to asthma severity. Although ORMDL3 is a member of a family that negatively regulates serine palmitoyltransferase (SPT) and thus biosynthesis of sphingolipids, it is still unclear whether ORMDL3 and altered sphingolipid synthesis are causally related to non‐Th2 severe asthma associated with a predominant neutrophil inflammation and high interleukin‐17 (IL‐17) levels. Here, we examined the effects of ORMDL3 overexpression in a preclinical mouse model of allergic lung inflammation that is predominantly neutrophilic and recapitulates many of the clinical features of severe human asthma. ORMDL3 overexpression reduced lung and circulating levels of dihydrosphingosine, the product of SPT. However, the most prominent effect on sphingolipid levels was reduction of circulating S1P. The LPS/OVA challenge increased markers of Th17 inflammation with a predominant infiltration of neutrophils into the lung. A significant decrease of neutrophil infiltration was observed in the Ormdl3 transgenic mice challenged with LPS/OVA compared to the wild type and concomitant decrease in IL‐17, that plays a key role in the pathogenesis of neutrophilic asthma. LPS decreased survival of murine neutrophils, which was prevented by co‐treatment with S1P. Moreover, S1P potentiated LPS‐induced chemotaxis of neutrophil, suggesting that S1P can regulate neutrophil survival and recruitment following LPS airway inflammation. Our findings reveal a novel connection between ORMDL3 overexpression, circulating levels of S1P, IL‐17 and neutrophil recruitment into the lung, and questions the potential involvement of ORMDL3 in the pathology, leading to development of severe neutrophilic asthma.
Original Research: Impact of a Bedside Activity Device on the Functional Status of Hospitalized Older Adults: A Randomized Controlled Trial
Authors: Monica Fan Peijin Esther , Louis Joshua Kenneth , Lim Xin Yi Cindy , Subramaniam Sarasuathi Gloria Encio , Seow Jason Phil , Aloweni Fazila , Rajasegeran Darshini Devi , Hsiang Cheng Chia , Thilarajah Shamala , Agus Nur Liyana , Ong Hwee Kuan , Ang Shin Yuh , Kannusamy Premarani
Published: February 2023

Background:

Older adults may have difficulty maintaining their functional capabilities during hospitalization. This pilot study aimed to investigate the impact of a bedside activity device on the functional status of hospitalized older adults.

Methods:

For this single-site randomized controlled trial, 48 participants were recruited between July 2019 and March 2021. Participants were randomized into one of two groups: the intervention group, which was given the use of a bedside activity device plus standard care, and the control group, which received standard care. Katz Index of Independence in Activities of Daily Living (Katz ADL) scores and Timed Up and Go (TUG) test times were used as indicators of functional status and were collected on admission (baseline) and at discharge.

Mann-Whitney U and χ2 tests were used to test for baseline similarities between groups. The Wilcoxon signed rank test was used to determine within-group pre–post changes in TUG and Katz ADL scores. The Mann-Whitney U test was used to determine between-group differences in TUG and Katz ADL change scores.

Results:

Within-group pre–post analysis showed significant increases in Katz ADL scores in the intervention group and no significant changes in the control group. TUG times decreased significantly in the intervention group and increased significantly in the control group. Between-group analyses showed significant differences in both TUG and Katz ADL change scores.

Conclusion:

The use of the bedside activity device in addition to standard care may prevent functional decline and increase independence in performing basic ADLs.
Fungal bioaerosols as an occupational hazard
Authors: Miller J. David
Published: February 2023

Purpose of review

Over the past 40 years, the circumstances where fungal bioaerosols are major issues have shifted because of changes in the industrial sector from mainly agriculture to operations, including composting, cannabis production and forestry in hot humid conditions. Changes in the design and operation of nonindustrial workplaces meant that mould and dampness became major issues that are just being reduced. This review attempts to frame that history offers a perspective on the current thinking on mechanisms and provide potentially useful sources of information for physicians and their patients.

Recent findings

The major impact of fungal exposures is not only from their allergens but also from an array of Danger-Associated Molecular Pattern molecules, possibly the most important of which is the type of glucan found in moulds that grow in damp buildings, wood chips and crops, that is beta 1, 3 D glucan in triple helical form. Located in lung epithelia, the dectin receptor is exquisitely sensitive to this compound. Except in some agricultural workplaces, low molecular weight secondary metabolites often mischaracterized as mycotoxins play little, if any, role on population health.

There has been a convergence in thinking between the allergy and industrial hygiene communities as well as government agencies on mould and occupational health. This has led to some useful strategies for better managing these issues as well as increasing consumer awareness.
Coronavirus disease 2019 and severe asthma
Authors: Benfante Alida , Pirrello Giuseppe , Sala Francesca , Seminara Gabriele , Scichilone Nicola
Published: February 2023

Purpose of review

The relationship between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and the most severe forms of asthma has been an object of discussion. Indeed, it is not clear whether asthma is among the risk factors for the occurrence of severe coronavirus disease 2019 (COVID-19) disease, or rather it plays a protective role against the worsening of the respiratory involvement in the SARS-CoV-2 infection. On the other hand, the extent to which coronavirus infection may trigger asthma attacks is still partly unknown. The current investigation aims at reviewing the available literature on the topic to address factors influencing this relationship.

Recent findings

Based on recent observations, it is likely that type 2 inflammation plays a protective role against SARS-CoV-2 infection and disease. In particular, asthmatics show different expression of angiotensin-converting enzyme2 (ACE2) and Transmembrane protease, serine 2 (TMPRSS2) that are responsible for a reduced risk of infection as well as lower risk of hospitalization. Interestingly, studies showed a safe profile of inhaled corticosteroids and biological drugs in SARS-CoV-2 infection. In addition, inhaled corticosteroid could play a protective role against worsening of asthma.

The current findings suggest that current treatment for asthma should be maintained to avoid severe exacerbations. Severe asthmatics under biological treatment should continue their medications, and be encouraged to receive COVID-19 vaccines.
A Longitudinal Perspective on Conversion to Amputation for Combat-Related Extremity Injuries Treated With Flap-Based Limb Salvage
Authors: Harrington Colin J. , Wade Sean M. , Hoyt Benjamin W. , Tintle Scott M. , Potter Benjamin K. , Souza Jason M.
Published: February 2023

Objectives:

To define the rate and primary drivers behind early and late amputation after flap-based limb salvage in the setting of combat extremity trauma.

Design:

Retrospective review.

Setting:

Level II trauma center.

Patients:

307 (303 men, 4 women) patients who underwent flap-based limb salvage treatment between 2003 and 2014.

Intervention:

We reviewed patient medical records, radiographs, and clinical photographs.

Main Outcome Measurements:

Early and late amputation rates, time to amputation, reason for amputation.

Results:

307 patients accounted for 323 limbs that underwent flap-based limb salvage treatment (187 lower extremities, 136 upper extremities). A total of 58 extremities (18%) initially treated with flap-based limb salvage ultimately underwent amputation at a median of 480 days (IQR, 285–715 days) from injury. Periarticular fractures and lower extremity injuries were risk factors for early and late amputation. Other independent risk factors for early amputation were flap complications and vascular injuries, whereas risk factors for late amputation were fractures that went on to nonunion.

Conclusions:

This study highlights that a subset of patients ultimately require major limb amputation despite having achieved what is initially considered “successful” limb salvage. Flap-related complications, vascular injury, and lower extremity site of injury were associated with early amputation after successful expeditionary efforts at limb preservation. Conversion to late amputation was associated with lower extremity periarticular fractures and fracture nonunion. Chronic pain and persistent limb dysfunction were the most common reasons for late amputation.

Level of Evidence:

Therapeutic Level IV. See Instructions for Authors for a complete description of levels of evidence.
Traumatic Posterior Atlantoaxial Dislocation With an Associated Fracture
Authors: Essa Ahmad , Khatib Salah , Beit Ner Eran , Smorgick Yossi , Mirovsky Yigal , Anekstein Yoram , Rabau Oded
Published: February 2023

Study design:

Systematic review.

Objective:

To investigate the clinical presentation, mechanism of injury, patients’ characteristics, diagnosis, treatment, and prognosis of traumatic posterior atlantoaxial dislocation with fracture (TPAD).

Background:

TPAD is an extremely rare entity, with a limited number of cases reported in the literature. Although rare, a thorough understanding of the pathogenesis and management of such cases is required due to the high risk of spinal cord compression and the severity of neurological deficits. We conducted a systematic review of all cases of TPAD with fractures reported in the literature.

Methods:

This systematic review was conducted following the Preferred Reporting Items for Systematic reviews and Meta-analysis guidelines. Relevant literature was retrieved from the Pubmed database. All published English-written studies involving human subjects with relevant data regarding TPAD were included.

Results:

Two case series and 20 case reports published between 1924 and 2021 describing 28 patients with TPAD were included. The median age at presentation was 51.5 years, with male predominance (83%). The most common fracture pattern was Anderson and D’alonzo type II odontoid fracture (78%). Neurological exam at presentation was intact in 52% of patients. The primary diagnostic imaging was computed tomography employed in 96% of cases. The management consisted of 2 stages protocol, initially, a temporary axial traction was employed (54%), and later definitive surgical treatment was employed (93%). The posterior surgical approach was favored in 82% of cases.

Conclusion:

TPAD with fracture occurred in the majority of cases with Anderson and D’alonzo type II odontoid fracture, predominantly in males. Diagnosis is usually established based on computed tomography imaging; further imaging may not be indicated as it does not seem to alternate the patient’s management course. Surgical intervention was employed in almost all cases, with favored results through posterior C1–C2 fusion.

Level of evidence:

Level—IV
Time to Awakening and Self-Fulfilling Prophecies After Cardiac Arrest
Authors: Elmer Jonathan , Kurz Michael C. , Coppler Patrick J. , Steinberg Alexis , DeMasi Stephanie , De-Arteaga Maria , Simon Noah , Zadorozhny Vladimir I. , Flickinger Katharyn L. , Callaway Clifton W. ,   For the University of Pittsburgh Post-Cardiac Arrest Service
Published: February 2023

OBJECTIVES:

Withdrawal of life-sustaining therapies for perceived poor neurologic prognosis (WLST-N) is common after resuscitation from cardiac arrest and may bias outcome estimates from models trained using observational data. We compared several approaches to outcome prediction with the goal of identifying strategies to quantify and reduce this bias.

DESIGN:

Retrospective observational cohort study.

SETTING:

Two academic medical centers (“UPMC” and “University of Alabama Birmingham” [UAB]).

PATIENTS:

Comatose adults resuscitated from cardiac arrest.

INTERVENTION:

None.

MEASUREMENTS AND MAIN RESULTS:

As potential predictors, we considered clinical, laboratory, imaging, and quantitative electroencephalography data available early after hospital arrival. We followed patients until death, discharge, or awakening from coma. We used penalized Cox regression with a least absolute shrinkage and selection operator penalty and five-fold cross-validation to predict time to awakening in UPMC patients and then externally validated the model in UAB patients. This model censored patients after WLST-N, considering subsequent potential for awakening to be unknown. Next, we developed a penalized logistic model predicting awakening, which treated failure to awaken after WLST-N as a true observed outcome, and a separate logistic model predicting WLST-N. We scaled and centered individual patients’ Cox and logistic predictions for awakening to allow direct comparison and then explored the difference in predictions across probabilities of WLST-N. Overall, 1,254 patients were included, and 29% awakened. Cox models performed well (mean area under the curve was 0.93 in the UPMC test sets and 0.83 in external validation). Logistic predictions of awakening were systematically more pessimistic than Cox-based predictions for patients at higher risk of WLST-N, suggesting potential for self-fulfilling prophecies to arise when failure to awaken after WLST-N is considered as the ground truth outcome.

CONCLUSIONS:

Compared with traditional binary outcome prediction, censoring outcomes after WLST-N may reduce potential for bias and self-fulfilling prophecies.