bluuebunny/crossref_metadata_2025 huggingface dataset:
Prismatic Dieudonné Theory
Authors: Anschütz Johannes , Le Bras Arthur-César
Published: January 2023

We define, for each quasisyntomic ringR(in the sense of Bhatt et al.,Publ. Math. IHES129(2019), 199–310), a category$\mathrm {DM}^{\mathrm {adm}}(R)$ofadmissible prismatic Dieudonné crystals over Rand a functor fromp-divisible groups overRto$\mathrm {DM}^{\mathrm {adm}}(R)$. We prove that this functor is an antiequivalence. Our main cohomological tool is the prismatic formalism recently developed by Bhatt and Scholze.
Reactive control of second Mack mode in a supersonic boundary layer with free-stream velocity/density variations
Authors: Nibourel Pierre , Leclercq Colin , Demourant Fabrice , Garnier Eric , Sipp Denis
Published: January 2023

We consider closed-loop control of a two-dimensional supersonic boundary layer at$M=4.5$that aims at reducing the linear growth of second Mack mode instabilities. These instabilities are first characterized with local spatial and global resolvent analyses, which allow us to refine the control strategy and to select appropriate actuators and sensors. After linear input–output reduced-order models have been identified, multi-criteria structured mixed$H_{2}$/$H_{\infty }$synthesis allows us to fix beforehand the controller structure and to minimize appropriate norms of various transfer functions: the$H_{2}$norm to guarantee performance (reduction of perturbation amplification in nominal condition), and the$H_{\infty }$norm to maintain performance robustness (with respect to sensor noise) and stability robustness (with respect to uncertain free-stream velocity/density variations). Both feedforward and feedback set-ups, i.e. with estimation sensor placed respectively upstream/downstream of the actuator, allow us to maintain the local perturbation energy below a given threshold over a significant distance downstream of the actuator, even in the case of noisy estimation sensors or free-stream density variations. However, the feedforward set-up becomes completely ineffective when convective time delays are altered by free-stream velocity variations of$\pm$5 %, which highlights the strong relevance of the feedback set-up for performance robustness in convectively unstable flows.
The association of parathyroid hormone with serum 25-hydroxyvitamin during pregnancy
Authors: Kazemian Elham , Madreseh Elham , Azizi Fereidoun , Ashrafivand Sepideh , Gargari Soraya Saleh , Mansournia Mohammad Ali , Wagner Carol L. , Amouzegar Atieh
Published: January 2023

It is currently debated whether vitamin D requirements during pregnancy differ from those during non-gravid states. In current analyses, we aimed to determine the best model for the association between PTH and serum 25-hydroxyvitamin D (25(OH)D) and the threshold for circulating 25(OH)D at which serum parathyroid hormone (PTH) is suppressed. This multicenter prospective cross-sectional study was conducted on 227 Iranian pregnant women aged 15–45 years in their third trimester of pregnancy. The locally weighted smoothing scatter plot (LOWESS) was used to determine the curvilinear shape of the 25(OH)D/PTH relationship. Linear and non-linear methods were employed to determine the best fit and cut-point for serum 25(OH)D concentration. The median serum 25(OH)D and corresponding serum PTH concentration were 17⋅26 (13⋅44–23⋅08) ng/ml and 19⋅46 (15⋅08–25⋅04) pg/ml in our study population, respectively. The LOWESS curve suggested a non-linear and monotonic with a negative slope relation between PTH (pg/ml) and serum 25(OH)D (ng/ml). The optimal model for the association between PTH and serum 25(OH)D was a one-term fractional polynomial (FP1) (AIC = 1640⋅463). The FP1 analysis identified the 25(OH)D threshold of 12⋅48 ng/ml at which serum PTH rapidly rose. The expected degree of PTH stimulation seems to have a linear trend as 25(OH)D falls below 40 ng/ml. 25(OH)D (ng/ml) and PTH (pg/ml) had a non-linear and monotonic relationship with a negative slope. Our data suggest that a 25(OH)D threshold of 12⋅48 ng/ml is sufficient for parathyroid hormone suppression, which could be used to screen for deficient individuals.
Parental background and daughters’ and sons’ educational outcomes – application of the Trivers-Willard hypothesis
Authors: Salminen Janne , Lehti Hannu
Published: January 2023

This study uses Trivers-Willard hypothesis to explain the differences in daughters’ and sons’ educational outcomes by parental background. According to the Trivers-Willard hypothesis (TWH), parental support and investments for sons and daughters display an asymmetrical relationship according to parental status because of the different reproductive advantage of the sexes. It predicts that high-status parents support sons more than daughters, and low-status parents support daughters more than sons. In modern societies, where education is the most important mediator of status, the TW hypothesis predicts that sons from high-status families will achieve higher educational outcomes than daughters. Using cohorts born between 1987 and 1997 from the reliable full population Finnish register data that contain the data of over 600.000 individuals, children’s educational outcomes were measured using data on school dropout rate, academic grade point average (GPA), and general secondary enrollment in their adolescence. OLS and sibling fixed-effect regression that permitted an examination of opposite-sex siblings’ educational outcomes within the same family were applied. Sons with high family income and parental education, compared to daughters of the same family, have lower probability of dropping out of school and are more likely to enroll into academic secondary school track. In families with low parental education or income daughters have lower probability for school dropout and enroll more likely to academic school track related to sons of the same family. The effect of family background by sex can be interpreted to support TWH in dropout and academic school track enrollment but not in GPA.
Adversarial scenarios for herding UAVs and counter-swarm techniques
Authors: Mendívez Vásquez Bruno L. , Barca Jan Carlo
Published: January 2023

The present paper aims to design and simulate an adversarial strategy where a swarm of quadrotor UAVs is herding anti-aircraft land vehicles (AALV) that actively oppose the swarm’s objective by potentially taking them down. The main strategy is to block the AALVs’ line of sight to their goal zone (AALVs’ objective), shifting its trajectory so it reaches a kill zone instead (UAVs’ objective). The counter-swarm strategy performed by the AALVs consists of taking down the closest aerial units to the goal zone. As a result, a consensus algorithm is executed by the UAVs in order to assess the communication network and re-group. Consensus is based on the propagation of local observations that converge into a global agreement on a communication graph. Re-grouping is done via positioning around the kill zone vector or preferring an anti-clockwise formation to better close gaps. The adversarial strategy was tested in an empty arena and urban setting, the latter making use of a path-planning procedure that re-routes the AALV trajectory based on its current destination. Simulation results show a maximum UAV mission success rate converging to roughly 80% in the empty arena. When targeted elimination procedures are executed, UAV mission performance drops 5%, making no distinction between re-grouping strategies in the empty arena. The urban setting shows lower performance due to navigation complexity but favors the decision to re-group based on a formation that close gaps rather than positioning around the kill zone vector.
A Further Fragment of the Abridged Version of Cassiodorus’s Commentary on the Psalms
Authors: Imhoff Helen
Published: January 2023

Hessisches Staatsarchiv Marburg Hr 2,19 is a recently described fragment of the abridged version of Cassiodorus’s Expositio psalmorum, as found in Durham, Cathedral Library, MS. B II. 30. In the past, it has been debated whether the abridged commentary ever existed in more than one copy, the focus being on the relationship between the fragment Landesbibliothek Düsseldorf K16:Z03/01 and Durham MS. B II. 30. This note argues that Hr 2,19 provides evidence that at least three or perhaps four copies existed. An appendix provides a transcription, images of the fragment, commentary on variants, and corrections made in Hr 2,19 and the Durham manuscript.
Direct healthcare costs of non-metastatic castration-resistant prostate cancer in Italy
Authors: Borsoi Ludovica , Ciani Oriana , Fornarini Giuseppe , Oderda Marco , Sciarra Alessandro , Vetrini Damir , Luccarini Irene
Published: January 2023

Objectives

The management of non-metastatic castration-resistant prostate cancer (nmCRPC) is rapidly evolving; however, little is known about the direct healthcare costs of nmCRPC. We aimed to estimate the cost-of-illness (COI) of nmCRPC from the Italian National Health Service perspective.

Methods

Structured, individual qualitative interviews were carried out with clinical experts to identify what healthcare resources are consumed in clinical practice. To collect quantitative estimates of healthcare resource consumption, a structured expert elicitation was performed with clinical experts using a modified version of a previously validated interactive Excel-based tool, EXPLICIT (EXPert eLICItation Tool). For each parameter, experts were asked to provide the lowest, highest, and most likely value. Deterministic and probabilistic sensitivity analyses (PSA) were carried out to test the robustness of the results.

Results

Ten clinical experts were interviewed, and six of them participated in the expert elicitation exercise. According to the most likely estimate, the yearly cost per nmCRPC patient is €4,710 (range, €2,243 to €8,243). Diagnostic imaging (i.e., number/type of PET scans performed) had the highest impact on cost. The PSA showed a 50 percent chance for the yearly cost per nmCRPC patient to be within €5,048 using a triangular distribution for parameters, and similar results were found using a beta-PERT distribution.

Conclusions

This study estimated the direct healthcare costs of nmCRPC in Italy based on a mixed-methods approach. Delaying metastases may be a reasonable goal also from an economic standpoint. These findings can inform decision-making about treatments at the juncture between non-metastatic and metastatic prostate cancer disease.
“An Exhibit as Will Astonish the Civilized World”: Seeking Separate Statehood for Indian Territory at the 1904 Louisiana Purchase Exposition
Authors: Crossley Laura
Published: January 2023

Chickasaw, Choctaw, Cherokee, Muscogee, and Seminole citizens employed the Indian Territory exhibits at the 1904 Louisiana Purchase Exposition to advance the separate statehood movement. Increasingly shut out of the formal political realm, they adopted creative measures to exert their political will, including participating in the world’s fair. Employing insights from settler-colonial theory and public history, this paper argues that the politics of display expanded the agency of a group marginalized from political representation. The U.S. government, pressured by the territory’s growing population of non-Native settlers, had begun planning for statehood, passing the 1898 Curtis Act to force allotment and dissolve the Five Tribes’ governments by 1906. To protect their land and sovereignty, a cohort of Native citizens pursued statehood for Indian Territory separate from Oklahoma Territory. Although joint statehood won out, separate statehood advocates succeeded in creating exhibits that centered on the survival of Native nations. They also articulated an Indigenous conception of citizenship, developing an imaginative vision for a future in which self-determination and U.S. citizenship could converge in a Native state. This represented a novel contribution to ongoing debates over how to integrate remaining western territories into the United States and how to incorporate diverse peoples within the citizenry.
Still Searching: A Black Family’s Quest for Equality and Recognition during the Gilded Age and Progressive Era
Authors: Broussard Albert S.
Published: January 2023

Historians have correctly interpreted the Gilded Age and Progressive Era as periods in which African Americans faced unpreceded violence, a significant decline in franchise, and the loss of many civil rights. These years however, were far more complex when viewed from the vantage point of African American families who attempted to empower themselves through education, securing employment in white-collar occupations, such as teaching, and working to advance themselves through race betterment groups, including women’s clubs and civil rights organizations. Yet some middle-class Black families like the Stewarts not only rejected white society’s widely held belief of Blacks as racially inferior and incapable of progress. They also embraced migration as a constructive strategy to advance their individual careers and to elevate the race. In an era when the majority of Black workers had minimal literacy and worked unskilled menial jobs, T. McCants Stewart and his children each graduated from college or professional school, worked in white-collar or professional jobs, and paved the way for the next generation. Yet each also understood that migration outside of the Jim Crow South, including to Africa, the U.S. Virgin Islands, and the territory of Hawaii, held the key to their success. Thus, the Stewarts constructed a new vision of freedom and opportunity and believed that even despite the repressive conditions imposed upon Blacks during the Gilded Age and Progressive Era that there was room for growth and an opportunity to advance their careers. Migration, therefore, should be reconsidered as a viable strategy that some Black families adopted to find their place in American society.
Characteristics of patients with multiple arterial aneurysms
Authors: Körfer Daniel , Erhart Philipp , Wortmann Markus , Dihlmann Susanne , Grond-Ginsbach Caspar , Kilian Samuel , Asatryan Ara , Jung Georg , Schmitz-Rixen Thomas , Böckler Dittmar , Hakimi Maani
Published: January 2023

Summary: Background: The aim of this retrospective cross-sectional observational study was to determine differences of patients with multiple arterial aneurysms to patients with single arterial aneurysms. Patients and methods: Patients with the diagnosis of an arterial aneurysm from January 2006 to January 2016 in the department of vascular surgery Heidelberg were investigated. Excluded were patients with hereditary disorders of connective tissue or systemic inflammatory disease, as well as other arterial pathologies than true aneurysms. Patients with multiple aneurysms (defined by at least four aneurysms) were compared to patients with single aneurysms concerning age at initial diagnosis, sex and affected arterial site. To verify the findings, a replication of the study was performed at a comparable institution. Results: Of 3107 patients with arterial aneurysms, 918 were excluded. Of the resulting 2189 patients, 1238 (56.6%) patients had a single, 808 (36.9%) two or three, and 143 (6.5%) at least four aneurysms (group mult-AA). Nine hundred seventy-two patients (44.4%) had a single abdominal aortic aneurysm (group sing-AAA). Age at initial diagnosis differed between mult-AA (66.7±9.5 y) and sing-AAA (69.1±8.6 y) (p=0.0338). Within mult-AA, 138 patients (96.5%) were male, compared with 865 patients (89.0%) in sing-AAA (p=0.0041). The most frequent aneurysm localization shifted from the abdominal aorta and its branches in patients with a single aneurysm (n=1029; 83.1%) to pelvic and leg arteries in patients with at least four aneurysms (n=318; 63.2%). The replication of the study at the department of vascular surgery Frankfurt confirmed the younger age at initial diagnosis in mult-AA (67.3±12.5 y) compared to sing-AAA (70.9±9.6 y) (p=0.0259) and the distribution shift toward the arteries below the aortic bifurcation in mult-AA. Conclusions: Patients with multiple aneurysms are younger at initial diagnosis and differ concerning aneurysm localization compared to patients with a single aneurysm.
Intensified Warming and Aridity Accelerate Terminal Lake Desiccation in the Great Basin of the Western United States
Authors: Hall Dorothy K. , Kimball John S. , Larson Ron , DiGirolamo Nicolo E. , Casey Kimberly A. , Hulley Glynn
Published: January 2023

Terminal lakes in the Great Basin (GB) of the western US host critical wildlife habitat and food for migrating birds and can be associated with serious human health and economic consequences when they desiccate. Water levels have declined dramatically in the last 100+ years due to diversion of inflows, drought and climate change. Satellite‐derived environmental science data records (ESDRs) from the MODerate‐resolution Imaging Spectroradiometer (MODIS) (snow cover, evapotranspiration (ET) and land surface temperature (LST)), enable a unique approach to evaluate the effects of aridification on terminal lakes and to study their individual vulnerabilities. Surface and air temperatures in the GB are rising dramatically, with a sharp rise in the rate of increase observed beginning around 2011, while the number of days of snow cover is declining especially in the western mountainous part of the GB as exemplified in Mono Basin, California. Rising temperatures coincide with fewer days of snow cover, a decrease of inflow to the lakes and greater evaporation of water from the lakes. MODIS ESDRs show strong and statistically significant increasing surface temperature (LST) in the GB, a reduction in the number of days of snow cover, and mixed results in ET. ET declined slightly in the more arid parts of the GB due to greater moisture restrictions to evaporation from extended drought, while ET increased in the more‐vegetated, wetter, mountainous northeastern parts as temperatures have risen. Severe and costly ecological, human health and economic consequences are expected if the lakes continue to decline as predicted.
Three mutations in SASH1 cause the pathogenesis of dyschromatosis universalis hereditaria (DUH)
Authors: Xing Qinghe , Zhou Dingan , Wei Zhiyun
Published: January 2023

Dyschromatosis universalis hereditaria (DUH) is a rare genodermatosis characterized by hyper- and hypopigmented macules which form a reticulate or mottled pattern. The causal gene and the precise pathogenesis of DUH have been unclear since the disease was initially reported in 1933. However, we found three heterozygous mutations encoding amino acid substitutions in SASH1 in each of three nonconsanguineous DUH families. Immunohistochemistry and melanin staining showed distribution heterogenicity of melanocytes, predominantly melanized melanocytes in the epidermal tissues of a DUH patient. Specifically, we identified that mutations of SASH1 can up-regulate resident melanogenic proteins, transport proteins of melanosome, and induce increased mobility of melanocytes in vitro and in vivo. Furthermore, SASH1 was shown to interact with several proteins associated with melanogenesis in the MAPK signaling pathway and the endothelin signaling pathway, indicating an additional melanogenesis signaling pathway in the regulation of melanin biosynthesis. Collectively, these observations suggest that DUH is a heterogeneous disorder of increased production and transport of melanosomes caused by SASH1 mutation together with melanocyte maldistribution.
Optimal storage and recall with biologically plausible synapses
Authors: Savin Cristina , Lengyel Mate
Published: January 2023

Synaptic plasticity is widely accepted to underlie learning and memory. Yet, models of associative networks with biologically plausible synapses fail to match brain performance: memories stored in such networks are quickly overwritten by ongoing plasticity (Amit & Fusi 1996, Fusi et al 2007). Metaplasticity - the process by which neural activity changes the ability of synapses to exhibit further plasticity - is believed to increase memory capacity (Fusi et al 2005). However, it remains unclear if neurons can make use of this additional information during recall. In particular, previous attempts at reading out information in metaplastic synapses using heuristic recall dynamics led to rather poor performance (Huang & Amit 2010).Here, we developed a theoretical framework for storage and recall with finite-state synapses that allowed us to find neural and synaptic dynamics that maximize the efficiency of autoassociative recall.Since information storage by synaptic plasticity is lossy, we formulated the problem of recalling a previously stored pattern from a noisy cue as probabilistic inference (Lengyel et al 2005) and derived neural dynamics efficiently implementing such inferences. Our approach is general and can be applied to any synaptic plasticity model which involves stochastic transitions between a finite set of states.We show how synaptic plasticity rules need to be matched to the statistics of stored patterns, and how recall dynamics need to be matched both to input statistics and to the plasticity rule itself in order to achieve optimal performance. In particular, for binary synapses with metastates we demonstrate for the first time that memories can be efficiently read out with biologically plausible network dynamics that we derive directly from the synaptic metaplasticity rule with virtually no free parameters.
Implantable intracortical microelectrodes: reviewing the present with a focus on the future
Authors: Wang Yang , Yang Xinze , Zhang Xiwen , Wang Yijun , Pei Weihua
Published: January 2023

Implantable intracortical microelectrodes can record a neuron’s rapidly changing action potentials (spikes). In vivo neural activity recording methods often have either high temporal or spatial resolution, but not both. There is an increasing need to record more neurons over a longer duration in vivo. However, there remain many challenges to overcome before achieving long-term, stable, high-quality recordings and realizing comprehensive, accurate brain activity analysis. Based on the vision of an idealized implantable microelectrode device, the performance requirements for microelectrodes are divided into four aspects, including recording quality, recording stability, recording throughput, and multifunctionality, which are presented in order of importance. The challenges and current possible solutions for implantable microelectrodes are given from the perspective of each aspect. The current developments in microelectrode technology are analyzed and summarized.
Mesenchymal stem cell-derived extracellular vesicles transfer miR-598 to inhibit the growth and metastasis of non-small-cell lung cancer by targeting THBS2
Authors: Li Xuebo , Wu Fan
Published: January 2023

Non-small-cell lung cancer (NSCLC) is the subtype of lung cancer, which accounts for about 85% of diagnosed lung cancer cases, and is without any effective therapy. Emerging evidence has revealed microRNA-598 (miR-598) as potential therapeutic target and diagnostic marker of NSCLC. In the present study, we sought to define the role of mesenchymal stem cells (MSCs)-derived extracellular vesicles (EVs) containing miR-598 in NSCLC. Co-culture experiments were conducted to examine the secretion of miR-598 by MSCs and the uptake of EVs by NSCLC cells. The expression of miR-598 in NSCLC cell lines, tissues, and MSC-derived EVs was detected by the RT-qPCR. After treatment with MSCs-EVs, CCK-8 and Transwell assays were adopted to evaluate the effects of miR-598 on proliferation, migration, and invasion capacities of NSCLC cells. Finally, the effects of miR-598 on tumor growth and metastasis were further validated in vivo through subcutaneous tumorigenesis and experimental pulmonary metastasis in nude mice. We found that MSCs-derived EVs could deliver miR-598 into NSCLC cells, where miR-598 specifically targeted and bound with mRNA of THBS2 to inhibit its translational process. By suppressing the promoting effects of THBS2 on the proliferation, migration, and invasion of NSCLC cells, the EV treatment reduced the progression of NSCLC. Notably, these inhibitory effects were reversed by concomitantly overexpressing THBS2. Overall, we find that MSCs-derived EVs containing miR-598 targets THBS2 to inhibit the proliferation and migration of NSCLC cells in vivo and in vitro.
Targeted systematic evolution of an RNA platform neutralizing DNMT1 function and controlling DNA methylation
Authors: Esposito Carla L. , Autiero Ida , Sandomenico Annamaria , Li H. , Bassal Mahmoud A. , Ibba Maria L. , Wang Dongfang , Rinaldi Lucrezia , Ummarino Simone , Gaggi Giulia , Borchiellini Marta , Swiderski Piotr , Ruvo Menotti , Catuogno Silvia , Ebralidze Alexander K. , Kortylewski Marcin , de Franciscis Vittorio , Di Ruscio Annalisa
Published: January 2023

DNA methylation is a fundamental epigenetic modification regulating gene expression. Aberrant DNA methylation is the most common molecular lesion in cancer cells. However, medical intervention has been limited to the use of broadly acting, small molecule-based demethylating drugs with significant side-effects and toxicities. To allow for targeted DNA demethylation, we integrated two nucleic acid-based approaches: DNMT1 interacting RNA (DiR) and RNA aptamer strategy. By combining the RNA inherent capabilities of inhibiting DNMT1 with an aptamer platform, we generated a first-in-class DNMT1-targeted approach – aptaDiR. Molecular modelling of RNA-DNMT1 complexes coupled with biochemical and cellular assays enabled the identification and characterization of aptaDiR. This RNA bio-drug is able to block DNA methylation, impair cancer cell viability and inhibit tumour growth in vivo. Collectively, we present an innovative RNA-based approach to modulate DNMT1 activity in cancer or diseases characterized by aberrant DNA methylation and suggest the first alternative strategy to overcome the limitations of currently approved non-specific hypomethylating protocols, which will greatly improve clinical intervention on DNA methylation.
Hidden heatwaves and severe coral bleaching linked to mesoscale eddies and thermocline dynamics
Authors: Wyatt Alex S. J. , Leichter James J. , Washburn Libe , Kui Li , Edmunds Peter J. , Burgess Scott C.
Published: January 2023

The severity of marine heatwaves (MHWs) that are increasingly impacting ocean ecosystems, including vulnerable coral reefs, has primarily been assessed using remotely sensed sea-surface temperatures (SSTs), without information relevant to heating across ecosystem depths. Here, using a rare combination of SST, high-resolution in-situ temperatures, and sea level anomalies observed over 15 years near Moorea, French Polynesia, we document subsurface MHWs that have been paradoxical in comparison to SST metrics and associated with unexpected coral bleaching across depths. Variations in the depth range and severity of MHWs was driven by mesoscale (10s to 100s of km) eddies that altered sea levels and thermocline depths and decreased (2007, 2017 and 2019) or increased (2012, 2015, 2016) internal-wave cooling. Pronounced eddy-induced reductions in internal waves during early 2019 contributed to a prolonged subsurface MHW and unexpectedly severe coral bleaching, with subsequent mortality offsetting almost a decade of coral recovery. Variability in mesoscale eddy fields, and thus thermocline depths, is expected to increase with climate change, which, along with strengthening and deepening stratification, could increase the occurrence of subsurface MHWs over ecosystems historically insulated from surface ocean heating by the cooling effects of internal waves.
Amazon windthrow disturbances are likely to increase with storm frequency under global warming
Authors: Feng Yanlei , Negrón-Juárez Robinson I. , Romps David M. , Chambers Jeffrey Q.
Published: January 2023

Forest mortality caused by convective storms (windthrow) is a major disturbance in the Amazon. However, the linkage between windthrows at the surface and convective storms in the atmosphere remains unclear. In addition, the current Earth system models (ESMs) lack mechanistic links between convective wind events and tree mortality. Here we find an empirical relationship that maps convective available potential energy, which is well simulated by ESMs, to the spatial pattern of large windthrow events. This relationship builds connections between strong convective storms and forest dynamics in the Amazon. Based on the relationship, our model projects a 51 ± 20% increase in the area favorable to extreme storms, and a 43 ± 17% increase in windthrow density within the Amazon by the end of this century under the high-emission scenario (SSP 585). These results indicate significant changes in tropical forest composition and carbon cycle dynamics under climate change.
Adaptive coding across visual features during free-viewing and fixation conditions
Authors: Nigam Sunny , Milton Russell , Pojoga Sorin , Dragoi Valentin
Published: January 2023

Theoretical studies have long proposed that adaptation allows the brain to effectively use the limited response range of sensory neurons to encode widely varying natural inputs. However, despite this influential view, experimental studies have exclusively focused on how the neural code adapts to a range of stimuli lying along a single feature axis, such as orientation or contrast. Here, we performed electrical recordings in macaque visual cortex (area V4) to reveal significant adaptive changes in the neural code of single cells and populations across multiple feature axes. Both during free viewing and passive fixation, populations of cells improved their ability to encode image features after rapid exposure to stimuli lying on orthogonal feature axes even in the absence of initial tuning to these stimuli. These results reveal a remarkable adaptive capacity of visual cortical populations to improve network computations relevant for natural viewing despite the modularity of the functional cortical architecture.
Quantum interference between dark-excitons and zone-edged acoustic phonons in few-layer WS2
Authors: Tan Qing-Hai , Li Yun-Mei , Lai Jia-Min , Sun Yu-Jia , Zhang Zhe , Song Feilong , Robert Cedric , Marie Xavier , Gao Weibo , Tan Ping-Heng , Zhang Jun
Published: January 2023

Fano resonance which describes a quantum interference between continuum and discrete states, provides a unique method for studying strongly interacting physics. Here, we report a Fano resonance between dark excitons and zone-edged acoustic phonons in few-layer WS2 by using the resonant Raman technique. The discrete phonons with large momentum at the M-point of the Brillouin zone and the continuum dark exciton states related to the optically forbidden transition at K and Q valleys are coupled by the exciton-phonon interactions. We observe rich Fano resonance behaviors across layers and modes defined by an asymmetry-parameter q: including constructive interference with two mirrored asymmetry Fano peaks (weak coupling, q > 1 and q < − 1), and destructive interference with Fano dip (strong coupling, ∣q∣ < < 1). Our results provide new insight into the exciton-phonon quantum interference in two-dimensional semiconductors, where such interferences play a key role in their transport, optical, and thermodynamic properties.
Computational conjugate adaptive optics microscopy for longitudinal through-skull imaging of cortical myelin
Authors: Kwon Yongwoo , Hong Jin Hee , Kang Sungsam , Lee Hojun , Jo Yonghyeon , Kim Ki Hean , Yoon Seokchan , Choi Wonshik
Published: January 2023

Myelination processes are closely related to higher brain functions such as learning and memory. While their longitudinal observation has been crucial to understanding myelin-related physiology and various brain disorders, skull opening or thinning has been required to secure clear optical access. Here we present a high-speed reflection matrix microscope using a light source with a wavelength of 1.3 μm to reduce tissue scattering and aberration. Furthermore, we develop a computational conjugate adaptive optics algorithm designed for the recorded reflection matrix to optimally compensate for the skull aberrations. These developments allow us to realize label-free longitudinal imaging of cortical myelin through an intact mouse skull. The myelination processes of the same mice were observed from 3 to 10 postnatal weeks to the depth of cortical layer 4 with a spatial resolution of 0.79 μm. Our system will expedite the investigations on the role of myelination in learning, memory, and brain disorders.
Brucella effectors NyxA and NyxB target SENP3 to modulate the subcellular localisation of nucleolar proteins
Authors: Louche Arthur , Blanco Amandine , Lacerda Thais Lourdes Santos , Cancade-Veyre Lison , Lionnet Claire , Bergé Célia , Rolando Monica , Lembo Frédérique , Borg Jean-Paul , Buchrieser Carmen , Nagahama Masami , Gérard Francine C. A. , Gorvel Jean-Pierre , Gueguen-Chaignon Virginie , Terradot Laurent , Salcedo Suzana P.
Published: January 2023

The cell nucleus is a primary target for intracellular bacterial pathogens to counteract immune responses and hijack host signalling pathways to cause disease. Here we identify two Brucella abortus effectors, NyxA and NyxB, that interfere with host protease SENP3, and this facilitates intracellular replication of the pathogen. The translocated Nyx effectors directly interact with SENP3 via a defined acidic patch (identified from the crystal structure of NyxB), preventing nucleolar localisation of SENP3 at late stages of infection. By sequestering SENP3, the effectors promote cytoplasmic accumulation of nucleolar AAA-ATPase NVL and ribosomal protein L5 (RPL5) in effector-enriched structures in the vicinity of replicating bacteria. The shuttling of ribosomal biogenesis-associated nucleolar proteins is inhibited by SENP3 and requires the autophagy-initiation protein Beclin1 and the SUMO-E3 ligase PIAS3. Our results highlight a nucleomodulatory function of two Brucella effectors and reveal that SENP3 is a crucial regulator of the subcellular localisation of nucleolar proteins during Brucella infection, promoting intracellular replication of the pathogen.
Searching for chromate replacements using natural language processing and machine learning algorithms
Authors: Zhao Shujing , Birbilis Nick
Published: January 2023

The past few years have seen the application of machine learning utilised in the exploration of materials. As in many fields of research—the vast majority of knowledge is published as text, which poses challenges in either a consolidated or statistical analysis across studies and reports. To address this issue, the application of natural language processing (NLP) has been explored in several studies to date. In the present work, we have employed the Word2Vec model, previously explored by others, and the BERT model—applying them towards the search for chromate replacements in the field of corrosion protection. From a database of over 80 million records, a down-selection of 5990 papers focused on the topic of corrosion protection were examined using NLP. This study demonstrates it is possible to extract knowledge from the automated interpretation of the scientific literature and achieve expert human-level insights.
Metavirome of 31 tick species provides a compendium of 1,801 RNA virus genomes
Authors: Ni Xue-Bing , Cui Xiao-Ming , Liu Jin-Yue , Ye Run-Ze , Wu Yu-Qian , Jiang Jia-Fu , Sun Yi , Wang Qian , Shum Marcus Ho-Hin , Chang Qiao-Cheng , Zhao Lin , Han Xiao-Hu , Ma Ke , Shen Shi-Jing , Zhang Ming-Zhu , Guo Wen-Bin , Zhu Jin-Guo , Zhan Lin , Li Liang-Jing , Ding Shu-Jun , Zhu Dai-Yun , Zhang Jie , Xia Luo-Yuan , Oong Xiang-Yong , Ruan Xiang-Dong , Shao Hong-Ze , Que Teng-Cheng , Liu Guang-Yuan , Du Chun-Hong , Huang En-Jiong , Wang Xin , Du Li-Feng , Wang Chong-Cai , Shi Wen-Qiang , Pan Yu-Sheng , Zhou Yu-Hao , Qu Jiang-Li , Ma Jiang , Gong Cai-Wei , Chen Qi-Qing , Qin Qian ,   Tick Genome and Microbiome Consortium (TIGMIC), Lam Tommy Tsan-Yuk , Jia Na , Cao Wu-Chun
Published: January 2023

The increasing prevalence and expanding distribution of tick-borne viruses globally have raised health concerns, but the full repertoire of the tick virome has not been assessed. We sequenced the meta-transcriptomes of 31 different tick species in the Ixodidae and Argasidae families from across mainland China, and identified 724 RNA viruses with distinctive virome compositions among genera. A total of 1,801 assembled and complete or nearly complete viral genomes revealed an extensive diversity of genome architectures of tick-associated viruses, highlighting ticks as a reservoir of RNA viruses. We examined the phylogenies of different virus families to investigate virome evolution and found that the most diverse tick-associated viruses are positive-strand RNA virus families that demonstrate more ancient divergence than other arboviruses. Tick-specific viruses are often associated with only a few tick species, whereas virus clades that can infect vertebrates are found in a wider range of tick species. We hypothesize that tick viruses can exhibit both ‘specialist’ and ‘generalist’ evolutionary trends. We hope that our virome dataset will enable much-needed research on vertebrate-pathogenic tick-associated viruses.
Gene gain facilitated endosymbiotic evolution of Chlamydiae
Authors: Dharamshi Jennah E. , Köstlbacher Stephan , Schön Max E. , Collingro Astrid , Ettema Thijs J. G. , Horn Matthias
Published: January 2023

Chlamydiae is a bacterial phylum composed of obligate animal and protist endosymbionts. However, other members of the Planctomycetes–Verrucomicrobia–Chlamydiae superphylum are primarily free living. How Chlamydiae transitioned to an endosymbiotic lifestyle is still largely unresolved. Here we reconstructed Planctomycetes–Verrucomicrobia–Chlamydiae species relationships and modelled superphylum genome evolution. Gene content reconstruction from 11,996 gene families suggests a motile and facultatively anaerobic last common Chlamydiae ancestor that had already gained characteristic endosymbiont genes. Counter to expectations for genome streamlining in strict endosymbionts, we detected substantial gene gain within Chlamydiae. We found that divergence in energy metabolism and aerobiosis observed in extant lineages emerged later during chlamydial evolution. In particular, metabolic and aerobic genes characteristic of the more metabolically versatile protist-infecting chlamydiae were gained, such as respiratory chain complexes. Our results show that metabolic complexity can increase during endosymbiont evolution, adding an additional perspective for understanding symbiont evolutionary trajectories across the tree of life.
Effects of syllable boundaries in Tibetan reading
Authors: Wang Danhui , Zeng Man , Zhao Han , Gao Lei , Li Shan , Niu Zibei , Bai Xuejun , Gao Xiaolei
Published: January 2023

Interword spaces exist in the texts of many languages that use alphabetic writing systems. In most cases, interword spaces, as a kind of word boundary information, play an important role in the reading process of readers. Tibetan also uses alphabetic writing, its text has no spaces between words as word boundary markers. Instead, there are intersyllable tshegs (“
”), which are superscript dots. Interword spaces play an important role in reading as word boundary information. Therefore, it is interesting to investigate the role of tshegs and what effect replacing tshegs with spaces will have on Tibetan reading. To answer these questions, Experiment 1 was conducted in which 72 Tibetan undergraduates read three-syllable-boundary conditions (normal, spaced, and untsheged). However, in Experiment 1, because we performed the experimental operations of deleting tshegs and replacing tshegs, the spatial information distribution of Tibetan sentences under different operating conditions was different, which may have a certain potential impact on the experimental results. To rule out the underlying confounding factor, in Experiment 2, 58 undergraduates read sentences for both untsheged and alternating-color conditions. Overall, the global and local analyses revealed that tshegs, spaces, and alternating-color markers as syllable boundaries can help readers segment syllables in Tibetan reading. In Tibetan reading, both spaces and tshegs are effective visual syllable segmentation cues, and spaces are more effective visual syllable segmentation cues than tshegs.
Predictive modeling of optimism bias using gray matter cortical thickness
Authors: Kotikalapudi Raviteja , Moser Dominik A. , Dricu Mihai , Spisak Tamas , Aue Tatjana
Published: January 2023

People have been shown to be optimistically biased when their future outcome expectancies are assessed. In fact, we display optimism bias (OB) toward our own success when compared to a rival individual’s (personal OB [POB]). Similarly, success expectancies for social groups we like reliably exceed those we mention for a rival group (social OB [SOB]). Recent findings suggest the existence of neural underpinnings for OB. Mostly using structural/functional MRI, these findings rely on voxel-based mass-univariate analyses. While these results remain associative in nature, an open question abides whether MRI information can accurately predict OB. In this study, we hence used predictive modelling to forecast the two OBs. The biases were quantified using a validated soccer paradigm, where personal (self versus rival) and social (in-group versus out-group) forms of OB were extracted at the participant level. Later, using gray matter cortical thickness, we predicted POB and SOB via machine-learning. Our model explained 17% variance (R2 = 0.17) in individual variability for POB (but not SOB). Key predictors involved the rostral-caudal anterior cingulate cortex, pars orbitalis and entorhinal cortex—areas that have been associated with OB before. We need such predictive models on a larger scale, to help us better understand positive psychology and individual well-being.
National-scale simulation of human movement in a spatially coupled individual-based model of malaria in Burkina Faso
Authors: Zupko Robert J. , Nguyen Tran Dang , Wesolowski Amy , Gerardin Jaline , Boni Maciej F.
Published: January 2023

Malaria due to the Plasmodium falciparum parasite remains a threat to human health despite eradication efforts and the development of anti-malarial treatments, such as artemisinin combination therapies. Human movement and migration have been linked to the propagation of malaria on national scales, highlighting the need for the incorporation of human movement in modeling efforts. Spatially couped individual-based models have been used to study how anti-malarial resistance evolves and spreads in response to drug policy changes; however, as the spatial scale of the model increases, the challenges associated with modeling of movement also increase. In this paper we discuss the development, calibration, and validation of a movement model in the context of a national-scale, spatial, individual-based model used to study the evolution of drug resistance in the malaria parasite.
Early evolution of glial morphology and inflammatory cytokines following hypoxic-ischemic injury in the newborn piglet brain
Authors: Teo Elliot J. , Chand Kirat. K. , Miller Stephanie M. , Wixey Julie A. , Colditz Paul B. , Bjorkman S. Tracey.
Published: January 2023

Neuroinflammation is a hallmark of hypoxic-ischemic injury and can be characterized by the activation of glial cells and the expression of inflammatory cytokines and chemokines. Interleukin (IL)-1β and tumor necrosis factor (TNF)α are among the best-characterized early response cytokines and are often expressed concurrently. Several types of central nervous system cells secrete IL-1β and TNFα, including microglia, astrocytes, and neurons, and these cytokines convey potent pro-inflammatory actions. Chemokines also play a central role in neuroinflammation by controlling inflammatory cell trafficking. Our aim was to characterise the evolution of early neuroinflammation in the neonatal piglet model of hypoxic-ischemic encephalopathy (HIE). Piglets (< 24 h old) were exposed to HI insult, and recovered to 2, 4, 8, 12 or 24H post-insult. Brain tissue from the frontal cortex and basal ganglia was harvested for assessment of glial cell activation profiles and transcription levels of inflammatory markers in HI piglets with comparison to a control group of newborn piglets. Fluorescence microscopy was used to observe microglia, astrocytes, neurons, degenerating neurons and possibly apoptotic cells, and quantitative polymerase chain reaction was used to measure gene expression of several cytokines and chemokines. HI injury was associated with microglial activation and morphological changes to astrocytes at all time points examined. Gene expression analyses of inflammation-related markers revealed significantly higher expression of pro-inflammatory cytokines tumor necrosis factor-α (TNFα) and interleukin 1 beta (IL-1β), chemokines cxc-chemokine motif ligand (CXCL)8 and CXCL10, and anti-inflammatory cytokine transforming growth factor (TGF)β in every HI group, with some region-specific differences noted. No significant difference was observed in the level of C-X-C chemokine receptor (CCR)5 over time. This high degree of neuroinflammation was associated with a reduction in the number of neurons in piglets at 12H and 24H in the frontal cortex, and the putamen at 12H. This reduction of neurons was not associated with increased numbers of degenerating neurons or potentially apoptotic cells. HI injury triggered a robust early neuroinflammatory response associated with a reduction in neurons in cortical and subcortical regions in our piglet model of HIE. This neuroinflammatory response may be targeted using novel therapeutics to reduce neuropathology in our piglet model of neonatal HIE.
Small molecule targeting long noncoding RNA GAS5 administered intranasally improves neuronal insulin signaling and decreases neuroinflammation in an aged mouse model
Authors: Patel Rekha S. , Lui Ashley , Hudson Charles , Moss Lauren , Sparks Robert P. , Hill Shannon E. , Shi Yan , Cai Jianfeng , Blair Laura J. , Bickford Paula C. , Patel Niketa A.
Published: January 2023

Shifts in normal aging set stage for neurodegeneration and dementia affecting 1 in 10 adults. The study demonstrates that lncRNA GAS5 is decreased in aged and Alzheimer’s disease brain. The role and targets of lncRNA GAS5 in the aging brain were elucidated using a GAS5-targeting small molecule NPC86, a frontier in lncRNA-targeting therapeutic. Robust techniques such as molecular dynamics simulation of NPC86 binding to GAS5, in vitro functional assays demonstrating that GAS5 regulates insulin signaling, neuronal survival, phosphorylation of tau, and neuroinflammation via toll-like receptors support the role of GAS5 in maintaining healthy neurons. The study demonstrates the safety and efficacy of intranasal NPC86 treatment in aged mice to improve cellular functions with transcriptomic analysis in response to NPC86. In summary, the study demonstrates that GAS5 contributes to pathways associated with neurodegeneration and NPC86 has tremendous therapeutic potential to prevent the advent of neurodegenerative diseases and dementias.