bluuebunny/crossref_metadata_2025 huggingface dataset:
The image of China in modern Russian media (based on materials from the National Corpus of the Russian Language)
Authors: Li Qizheng
Published: May 2024

The article examines the representation in the language of modern media of the image of China as a component of Russian linguistic consciousness. It is noted that in the language of the media, the image of China that has developed in the Russian linguistic consciousness is simultaneously realized, and this image is constructed and supplemented in its modern implementation. The media have a significant impact on the image of China, what it will be like in the future, and also on how Russians perceive it. The analysis was based on the consideration of the functioning in the media of lexemes and phraseological units expressing Chinese semantics: China, Chinese, Chinaman, Celestial Empire, hieroglyphs, etc. Observation showed that the image of China is frequent. The research was carried out on the basis of traditional methods of observation, description, analysis, synthesis, and generalization. One of the main ones was the method of corpus linguistics. The method of linguocultural interpretation, structural-semantic method were used. It has been revealed that the most important part of the image of China, implemented in the media, is the idea of this country as a strong state with developed industry, economy and social sphere, as a strategic partner of the Russian Federation and a global antagonist of the United States. Attention is focused on the fact that the brightest means of representing the image of China in the Russian linguistic consciousness are comparative constructions, metaphors, phraseological units, through which such features of China as ancient history, rich and difficult to understand culture, and deep philosophy are actualized. A Chinese in the Russian linguistic consciousness is a hardworking, enterprising, intelligent, cunning, wise, independent person. The conclusion is made about the dynamic image of China, to the change of which the Russian media make a significant contribution.
Refiguring in Black, by Tendayi Sithole
Authors: Masola Athambile
Published: May 2024

Refiguring in Black, by Tendayi Sithole
Polity Press. 2023. viii + 158.
ISBN: HB: 13: 978-1-5095-5071-1; PB: 13: 978-1-5095-5702-8
 
Athambile Masola
https://orcid.org/0000-0001-9550-7944
University of Cape Town
athambile.masola@uct.ac.za
Hybridisation as a Normal Process of Life: A Contribution to the “Ukuthwasa” Conversation within the Methodist Church of Southern Africa
Authors: Mokhutso Jacob
Published: May 2024

The Methodist Church of Southern Africa (MCSA) is currently engaged in a conversation on ukuthwasa (initiation into an ancestral calling). The conversation has sparked different reactions within the Church. Some are uncomfortable engaging in this conversation as it is seen as an unnecessary and unChristian conversation to have within the Church. There are also some who have gone through the ukuthwasa and feel that the conversation is long overdue within the MCSA. Furthermore, some believe in sangomas and are happy about this conversation. While this conversation is taking place within the MCSA, it is shrouded in suspicion and fear. There is fear that it might lead to the conversion of the Church to African traditional religion, which many feel is the opposite of Christianity. This paper is intended as a contribution to this conversation by using secondary or desk research as a methodology. Firstly, the paper defines the African worldview in which ukuthwasa is embedded. Secondly, it explores the meaning of hybridisation. Thirdly, it indicates areas within the Christian faith where hybridisation has become part of worship and belief. Finally, the paper concludes by recommending openness in this conversation as a likely solution that could lead to the Methodist vision of a “Christ healed Africa for the healing of nations.”
Congenital absence of anterior and posterior cruciate ligaments – A rare case report and review of literature
Authors: Gavvala Sai Niharika , Giliyaru Sahana , Ariyaratne Sisith , Panchal Hiten , Iyengar Karthikeyan P. , Botchu Rajesh
Published: May 2024

A rare occurrence, congenital absence of the cruciate ligaments has a prevalence of 0.017/1000 live births. A literature review on the PubMed database revealed only a few case reports of both coexisting anterior cruciate ligament and posterior cruciate ligament congenital absence. Most cases were found to be associated with other congenital abnormalities, making isolated congenital absence of the cruciate ligaments exceptionally rare. Liu et al. reported a total of nine cases to date, with our case being the second youngest and having no other associated knee pathologies. Our report presents a 4-year-old girl with unilateral congenital absence of a cruciate ligament, diagnosed through magnetic resonance imaging. It is crucial to be aware of this rare abnormality for proper management and to minimize morbidity.
Nucleobase catalysts for the enzymatic activation of 8-oxoguanine DNA glycosylase 1
Authors: Hank Emily C. , D'Arcy-Evans Nicholas , Scaletti Emma Rose , Benítez-Buelga Carlos , Wallner Olov , Ortis Florian , Zhou Kaixin , Meng Liuzhen , Calvo Patricia , Almlöf Ingrid , Wiita Elisée , Kosenina Sara , Krämer Andreas , Long Maeve , Jemth Ann-Sofie , Dawson Holly , Stewart Josephine , Dickey Adam , Astorga Mikhael E. , Varga Marek , Homan Evert J , Scobie Martin , Knapp Stefan , de Vega Miguel , Sastre Leandro , Stenmark Pål , Helleday Thomas , Michel Maurice
Published: May 2024

: Bifunctional DNA glycosylases employ an active site lysine or the N-terminus to form a Schiff base with the abasic site (AP site) base excision repair (BER) intermediate. Cleaving this reversible structure is the rate-determining step in the initiation of 8-oxoguanine (8-oxoG) repair for 8-oxoguanine DNA glycosylase 1 (OGG1). The OGG1 AP lyase activity can be increased using small molecule binders, called organocatalytic switches, to cleave the DNA backbone in a similar manner as a bifunctional DNA glycosylase. In search for novel organocatalytic switches we here identify 8-Substituted 6 thioguanines and 6-amino-pyrazolo-[3,4-d]-pyrimidine derivatives as potent and selective scaffolds enabling OGG1 to cleave AP sites opposite any canonical nucleobase by β-elimination, shaping a complete, artificial AP-lyase function. These new tool compounds enhance the cellular repair of 8-oxoG and AP sites, activating a rudimentary but canonical enzymatic activity.
Multistep Library Synthesis in Flow: Production of Matrix Libraries in an Assembly Line
Authors: Pijper Brenda , Abdiaj Irini , Mazuela Javier , Linares Maria Lourdes , Gomez Jose Enrique , Rodriguez Raquel , Chavez Arquero Belén , Palao Eduardo , Cañellas Santiago , Alcazar Jesus
Published: May 2024

In drug discovery, automated library synthesis has traditionally focussed on established chemistry such as amide formation or Suzuki coupling. However, to explore a broader chemical space, recent automated protocols have emphasized on increasing the fraction of sp3 hybridized carbons to move beyond the limitations of planar molecules. Herein we present a novel approach for multistep library synthesis in continuous flow, enabling the linkage of three different elements in a combinatorial way. To achieve this synthesis, we employed nine different reactions in various combinations. Initially, we constructed a full combinatorial library, followed by the development of multi-transformation libraries where disparate reactions were integrated into a single experimental setup, prioritizing chemistries capable of introducing C(sp3) fragments while maintaining efficiency. By integrating diverse reactions and optimization capabilities, our method offers a powerful means to expand chemical diversity in drug discovery libraries.
Zirconium Hydride Catalysis Initiated by Tetrabutylammonium Fluoride
Authors: Harwood Stephen , Aloiau Athenea , Bobek Briana , Pearson Kelly , Cherry Kendall , Smith Christopher , Ketcham John , Marx Matthew
Published: May 2024

In our drug discovery campaigns to target the oncogenic drivers of cancers, the demand for a chemoselective, stereoselective and economical synthesis of chiral benzylamines drove the development of a catalytic zirconium hydride reduction. This methodology uses the inexpensive, bench stable zirconocene dichloride, and a novel tetrabutylammonium fluoride activa-tion tactic to catalytically generate a metal hydride under ambient conditions. The diastereo- and chemoselectivity of this reaction was tested with the preparation of key intermediates from our discovery programs and in the scope of sulfinyl ketimines and carbonyls relevant to medicinal chemistry and natural product synthesis. A preliminary mechanistic investi-gation conducted into the role of tetrabutylammonium fluoride indicates that formation of a zirconocene fluoride occurs to initiate catalysis. The implications of this convenient activation approach may provide expanded roles for zirconium hy-drides in catalytic transformations.
Can qualitative non-target data be indicative of PFAS contamination? First evidence by correlation with EOF in environmental samples
Authors: Zweigle Jonathan , Simon Fabian , Meermann Björn , Zwiener Christian
Published: May 2024

We combined and correlated non-target screening (NTS) features and extractable organic fluorine (EOF) data for PFAS analysis in environmental samples. Soils, sediments, and ashes from different locations (Germany, France, Lithuania) (n=34) were extracted and analyzed via liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and high resolution-continuum source-graphite furnace molecular absorption spectrometry (HR-CS-GFMAS). NTS feature prioritization based on the mass to carbon (m/C) and mass defect to carbon (MD/C) ratio was applied to discriminate potential PFAS features from common co-extracted organic matrix. Our results show a high correlation between quantitative EOF data with the prioritized feature number (R2 = 0.87, rPearson = 0.93) as well as with the total peak area of prioritized features (R2 = 0.97, rPearson = 0.98) while commonly applied MD filtering showed no correlation with EOF. The observed highly significant correlations highlight that MD/C-m/C prioritization is associated with a good indication of the overall PFAS burden as evidenced by EOF. Also, semi-quantitative data can be extracted from PFAS-specific NTS as shown for the EOF vs. prioritized peak areas. The correlations indicate that lowly fluorinated compounds were negligible and that the EOF was mostly composed of higher fluorinated substances. We recommend further investigation of the unique complementary EOF vs. NTS combination for PFAS screening.
Time-Dependent Kohn-Sham Electron Dynamics Coupled with Nonequilibrium Plasmonic Response via Atomistic Electromagnetic Model
Authors: Huang Xunkun , Zhang Wenshu , Liang WanZhen
Published: May 2024

Computational modeling of plasmon-mediated molecular photophysical and photochemical behaviours can help us better understand and tune the bound molecular properties and reactivity, and make better decisions to design and control nanostructures. However, computational investigations of coupled plasmon-molecule systems are challenging due to the lack of accurate and efficient protocols to evaluate these systems. Here we present a hybrid scheme by combining real time time-dependent density functional theory (RT-TDDFT) method with time-domain frequency dependent fluctuating charge (TD-ωFQ) model. At first, we transform ωFQ, which was formulated in the frequency domain, to time-domain and derive its equation-of-motion formulation. The TD-ωFQ introduces the nonequilibrium plasmonic response of metal nanoparticle (MNP) and atomistic interactions to the electronic excitation of QM region. Then we combine TD-ωFQ with RT-TDDFT. The derived RT-TDDFT/TD-ωFQ scheme allows us to effectively simulate the plasmon-mediated “real-time” electronic dynamics and even the coupled electron-nuclear dynamics by combining with the nuclear dynamics approaches. As a first application of the RT-TDDFT/TD-ωFQ method, we study the nonradiative decay rate and plasmon-enhanced absorption spectra of two small molecules in the proximity of sodium MNPs. Thanks to the atomistic nature of ωFQ model, the edge effect of MNP to absorption enhancement has also been investigated. and unveiled.
A 2D Array of Copper(I) Catenane Catalyst Grafted on a Metal-Organic Layer for High Fidelity Cross-Coupling
Authors: Shi Yi-Xiang , Zhu Lihui , Deng Yulin , Mo Xiaoyong , Wang Yufeng , Tse Edmund Chun Ming , Au-Yeung Ho Yu
Published: May 2024

While Earth abundant metals are green and sustainable alternatives to precious metals for catalytic chemical conversions, the fast ligand exchange involving most of these metals renders their development into robust, reusable catalysts very challenging. Described in this work is a new type of heterogeneous catalyst derived from a 2D metal-organic layer (MOL) grafted with catenane-coordinated Cu(I) complexes. In addition to the good substrate accessibility, easy functionalization and other favorable features due to the MOL support, the anchored catenane ligands also provide a well-defined coordination environment and good kinetic stability to the coordinated Cu(I). Catalytic studies using phenols and bromodicarbonyls as the substrates showed that the Cu(I) catenane-grafted MOL resulted in the exclusive C–O coupling of the substrates, whereas a control catalyst in which the catenanes are replaced by non-interlocked macrocyclic ligands was found to lead to also a C–C coupling due to the uncontrolled formation of oxidized copper active site. The integration of mechanically interlocked catalyst to extended framework support thus represent an unexplored potential of exploiting labile, Earth-abundant metals for sustainable catalysis under challenging conditions.
Efficient generation of open multi-stage fragmentation mass spectral libraries
Authors: Brungs Corinna , Schmid Robin , Heuckeroth Steffen , Mazumdar Aninda , Drexler Matúš , Šácha Pavel , Dorrestein Pieter C. , Petras Daniel , Nothias Louis-Felix , Nencka Radim , Kameník Zdeněk , Pluskal Tomáš
Published: May 2024

Untargeted analysis based on high-resolution mass spectrometry is a key tool in clinical metabolomics, natural product discovery, and exposomics, with compound identification remaining the major bottleneck. Currently, MS2 fragmentation data and spectral library matching are the standard workflow for confident compound annotation. Multi-stage fragmentation (MSn) yields more profound insights into substructures, enabling validation of fragmentation pathways; however, the community lacks open MSn data for reference compounds. Here, we describe a high-throughput method for acquiring MSn trees and an automated workflow for extracting and building open MSn libraries. By applying this pipeline to ~ 20,600 small molecules, we obtained MSn spectra for 16,391 unique compound structures in twelve days. This resource includes 1,126,997 MSn spectra and can be leveraged for compound annotation based on library matching, including substructures and training of machine learning models on substructure-fragmentation patterns. The workflow, implemented in mzmine and Python scripts, is open-source and freely available to anyone interested.
Hierarchical Assembly and Environmental Enhancement of Bacterial Ice Nucleators
Authors: Renzer Galit , de Almeida Ribeiro Ingrid , Guo Hao-Bo , Fröhlich-Nowoisky Janine , Berry Rajiv , Bonn Mischa , Molinero Valeria , Meister Konrad
Published: May 2024

Bacterial ice nucleating proteins (INPs) are exceptionally effective in promoting the kinetically hindered transition of water to ice. Their efficiency relies on the assembly of INPs into large functional aggregates, with the size of ice nucleation sites determining activity. Experimental freezing spectra have revealed two distinct, defined aggregate sizes, typically classified as class A and C ice nucleators (INs). Despite the importance of INPs and years of extensive research, the precise number of INPs forming the two aggregate classes and their assembly mechanism have remained enigmatic. Here, we report that bacterial ice nucleation activity emerges from more than two prevailing aggregate species and identify the specific number of INPs responsible for distinct crystallization temperatures. We find that INP dimers constitute class C INs, tetramers class B INs, and hexamers and larger multimers are responsible for the most efficient class A activity. We propose a hierarchical assembly mechanism based on tyrosine interactions for dimers, and electrostatic interactions between INP dimers to produce larger aggregates. This assembly is membrane-assisted: increasing the bacterial outer membrane fluidity decreases the population of the larger aggregates, while preserving the dimers. Inversely, DPBS buffer increases the population of multimeric class A and B aggregates 200-fold and endows the bacteria with enhanced stability towards repeated freeze-thaw cycles. Our analysis suggests that the enhancement results from the better alignment of dimers in the negatively charged outer membrane, due to screening of their electrostatic repulsion. This constitutes the first demonstration of enhancement of the most potent bacterial INs.
Calculation of the relative permittivity of Rhodamine 6G using the quantum mechanical method
Authors: Riabenko Iuliia , Shulga Sergey , Beloshenko Konstantin
Published: May 2024

Background: The article describes a method for calculating the permittivity of organic molecules in quantum mechanics using the well-studied Rhodamine 6G molecule as an example. The study of optical properties of large organic molecules requires not only experimental data but also the use of calculations obtained both analytically and numerically.

Objectives: Methods for calculating permittivity as phenomenological characteristics of a sample are to be tested on well-studied molecules to be further applied to more complex nonlinear structures. However, the integral changes need to be approximated in the wave functions of large molecules.

Material and methods: The numerical simulations in MATLAB were carried out to be compared with the data from Gaussian 09, which are accurate for such small molecules as Rhodamine 6G. MATLAB calculated permittivity values for the frequency domains corresponding to absorption and fluorescence based on the Fermi golden rule. Hence, any molecule can be represented as a composite quantum mechanical system. Meanwhile, Gaussian 09 used the DFT method to determine permittivity.

Results: The Fermi golden rule can be applied due to the representation of the molecule as a complex quantum mechanical system. The proposed numerical methods minimize error by using the Dirac delta function. According to our hypothesis, the sum of the wave function of a particle in a potential well and a particle in a ring equals the wave function of the entire system, thus making it possible to study large molecules. As a result of the calculation for two wavelengths of 337 and 573 nm, the permittivity results calculated using the proposed method in MATLAB are 2.98 and 6.27, respectively. Gaussian 09 calculated the same parameters at 2.85 and 6.23.

Conclusion: The resulting datasets show a high degree of correlation. Therefore, the research hypothesis has been confirmed. The selected method also proved efficient, hence the enhancement of luminescence can be achieved by changing the relaxation time of the excited state. Plasmonic nanostructures with predetermined properties will controllably enhance the resulting field by the square of the superposition modulus of their near-field. Consequently, conditions for highly coherent radiation with high intensity and polarization can be predicted and calculated before an experiment is carried out.
C60 fullerene nanoparticles permeability through the model lipid envelope of coronavirus and their anticoronavirus effect in the in ovo system
Authors: Hurmach Vasyl , Karaushu Vyacheslav , Klestova Zinaida , Berest Volodymyr , Prylutskyy Yurii
Published: May 2024

Background: An urgent problem of modern biomedicine is the search for potential ways to reduce the spread of coronavirus infections in human and animal populations and eliminate the impact of coronavirus on their bodies.

Aim of the work was to determine the structural organization of C60 fullerenes in a model cell medium, to assess their potential ability to penetrate the model lipid envelope of a coronavirus, thus destroying its integrity, as well as the effectiveness of the anticoronavirus action in the in ovo system.

Methods: The research was conducted using the Gromacs 2020 software package and the Charmm36 force field. The CHARMM-GUI web resource was used to construct the lipid envelope of a coronavirus. Virological and toxicological methods were used in the in ovo experiments.

Results: It is shown that at the initial stages of molecular dynamics C60 fullerene forms small nanoclusters in the amount of 7–16 molecules, which is affected by the initial concentration of C60 fullerene in a model cell medium. At the same time, the shape of nanostructures for the number of molecules over 16 may differ from the correct spherical shape. It was established that C60 fullerene and its nanoclusters are able to penetrate inside the lipid envelope of a coronavirus. In particular, a single C60 molecule can migrate, completely embed itself in the lipid envelope, or move within it. It was found that C60 fullerene aqueous solution, at the maximum permissible concentration (MPC) of 30 μg/embryo, reduces the infectious activity of the chicken infectious bronchitis (CIB) coronavirus when it is used 1, 2 and 4 h after the infection of a sensitive biological system with a coronavirus.

Conclusions: It is shown that aggregation of C60 fullerene in a model cell medium can occur in different ways: either before penetration into the lipid envelope of a coronavirus, or inside it. C60 fullerenes, both individually and as part of nanoclusters, are able to form pores in the envelope of a coronavirus, destroying its integrity, which possibly leads to disruption of the corresponding stages of its replication cycle and attachment to cell receptors. It was established that C60 fullerene aqueous solution, when used in the MPC value, shows antiviral activity against the CIB coronavirus in its early stages of interaction with the cell (1–4 h).
Age changes in the lipid profile of rats against experimental metabolic syndrome
Authors: Kuzmina I.Yu. , Kuzmina O.O.
Published: May 2024

Background. Metabolic syndrome (MS) is characterized by the development of pathogenetic mechanisms that potentiate abdominal obesity and insulin resistance. Obesity is a stimulator of the synthesis of active components of adipose tissue – adipocytes (AT), which leads to numerous metabolic and hemodynamic disorders. The study of pathophysiological mechanisms based on the basis of morphometric and biochemical parameters of blood pressure, taking into account age and gender aspects, is of great topical importance. Purpose – of the study is to identify differences in the morphological and histological parameters of adipocytes against the background of the development of metabolic syndrome in animals of different ages and sexes. Materials and Methods. To achieve the goal, an experimental study was conducted on 108 white rats of the WAG/G Sto population. Each age group consisted of a control (36) and an experimental series (72), where the animals were divided by sex. The 1st group included young immature 3-month-old rats of the control (1K) and experimental (1A) subgroups. Group 2 consisted of 36 sexually mature rats aged 5–6 months, divided into 2 subgroups: control (2K) and experimental (2A). The third group consisted of 36 old rats aged 18 months, divided into the control 3K (12) subgroup (6 old females and 6 old males), and the experimental subgroup 3A – of 12 females and 12 males. Results. The hypodermic tissue of all experimental groups consists of adipocytes, the size, shape and density of which had age and gender differences. In group 1A of both subgroups, the cells had a round and oval shape and were significantly smaller in size compared to groups 2A and 3A. Adipocytes of rats have morphological changes against the background of experimental modeling of MS in taking into account age and gender aspects. It was established that in the group of old animals, hypertrophic processes prevail in BP, against the background of greatly reduced hyperplastic processes. Analyzing and comparing the size of adipose tissue cells between experimental groups 1A, 2A and 3A, the dependence of the increase in the size of adipocytes, which is related to the age of the animal, was noted. Conclusions. Experimental changes in the integrative indicators of AT of rats in the pathogenesis of MS are related to age and sex. An inversely proportional dependence of the decrease in the resistance of adipocytes as the age of the animal increases was noted. The obtained results can be designated as a biological marker that determines the development of obesity against the background of disorders of the systemic inflammatory response and insulin resistance.
Clinical features of cognitive dysfunction in patients with relapsing-remitting type of multiple sclerosis
Authors: Teslenko O.S. , Tovazhnyanska O.L.
Published: May 2024

Background. Cognitive dysfunction in patients with multiple sclerosis is quite common, but attention is not always paid to it, since the decline of cognitive functions is often masked by motor, sensory, and visual disorders. Active patient questioning and neurocognitive screening are needed to identify cognitive impairment in patients with multiple sclerosis, even in the early stages of the disease. The goal of the study is to determine the frequency, severity, and clinical features of cognitive impairment in patients with relapsing-remitting multiple sclerosis, taking into account the duration of the disease and the level of disability of the patients. Materials and Methods. 67 patients with a diagnosis of relapsing-remitting multiple sclerosis were examined. All examined patients underwent a thorough neurological, psychometric, and instrumental examination. Patients were divided into 3 groups depending on the duration of the disease: 1st group up to 5 years (24 patients), 2nd group – from 5 to 10 years (22 patients), 3rd group more than 10 years (21 patients). The Symbol Digit Modalities Test (SDMT) and the Montreal Cognitive Function Assessment Scale (MoCA) were used to assess patients’ neuropsychological status. Results. The conducted correlation analysis showed the presence of a probable inverse relationship between the score on the EDSS scale and the scores on the SDMT and MoSA scales (r = –0.61 (p0.05); r = –0.12 (p>0.05) for scores on SDMT and MoCA scales, respectively). We also obtained a probable directly proportional correlation between the test scores on the MoСA scale and SDMT (in 1st group = 0.63, p<0.05, in 2nd group = 0.89, p<0.05, in the 3rd group r = 0.64, p<0.05) in all studied groups, i.e. for all periods of the disease duration.

Conclusions. The obtained data of the correlation analysis indicate a relationship between the severity of cognitive impairment according to the test scores, the degree of disability of the patients, and the duration of the disease.
Preparation of the endometrium in the programs of auxiliary reproductive technologies for sterility in women
Authors: Lutskyi A.S.
Published: May 2024

Background. The most important stage in the treatment of infertility is the introduction into clinical practice of the methods of in vitro fertilization (IVF), which made it possible to implement the function of childbirth in almost all forms of female infertility. The introduction of endoscopic technologies made it possible to significantly expand diagnostic possibilities and clarify the nature of intrauterine pathology when using assisted reproductive technologies. Purpose – of the research should be to increase the effectiveness of the treatment of female infertility when using assisted reproductive technologies and hysteroscopy by creating conditions in the endometrium for embryo implantation. Materials and Methods. A study was conducted in 42 patients with infertility aged 32 to 38 years. In all women, the morphological state of the endometrium was determined after diagnostic hysteroscopy. The patients were divided into 2 clinical groups. Group 1 included 28 women who were in a state of preparation for IVF after hysteroscopy with changes characteristic of endometrial pathology, which were found during ultrasound. The second group included 14 patients in whom, according to ultrasound, no structural changes of the endometrium were noted. These patients had a failed IVF attempt. In the next cycle, the women underwent a diagnostic hysteroscopy due to the lack of pregnancy after the first IVF attempt. Results. Group 1 patients (n = 28) with endometrial changes before IVF showed that the causes of pathology were: endometrial hyperplasia (53.6%); chronic endometritis (21.4%), endometriosis (25%). Therefore, before stimulation of superovulation, a hysteroscopy was performed to confirm the morphological diagnosis. In patients of the 2nd group (n = 14) after hysteroscopy and histological examination, pathological changes of the endometrium were found in all patients. Chronic endometritis occurred in 5 cases (35.7%), micropolyposis of the endometrium – in 6 (42.9%), endometriosis – 3 (21.4%). Clinical pregnancy occurred in 17 patients (60.7%) in the first group, in 9 (64.2%) in the second group. Conclusions. Hysteroscopy with morphological examination of the endometrium remains the main method of diagnosing intrauterine pathology. Diagnosis of infertility by hysteroscopy plays a very important role in the normalization of reproductive function during in vitro fertilization (IVF). After an unsuccessful IVF attempt, in the absence of pathological changes in the endometrium, a diagnostic hysteroscopy should be performed to rule out endometrial pathology.
Cardiovascular complications of COVID-19
Authors: Bila N.V. , Brynza M.S. , Tytarenko N.V.
Published: May 2024

Background. The problem of COVID-19 today is the most unresolved worldwide. Scientists and doctors all over the world are trying to study features of pathogenesis this disease to find the most effective treatment methods. Multiple organ complications COVID-19 lead to death and disability of patients. The disease manifests itself with symptoms of damage to the pulmonary system with subsequent damage other systems and organs. Considering that cardiovascular diseases remain the leading cause of death worldwide, it is necessary to study the features of flow COVID-19 against the background of existing cardiovascular pathology. Purpose – the purpose of the study was to analyze and summarize literature sources, which are devoted to the study of damage to the cardiovascular system as a result of COVID-19 affection. Materials and Methods. Publications were selected using keywords in PubMed (https://pubmed.ncbi.nlm.nih.gov/) and Google Scholar (https://scholar.google.com/) databases published over the period from 2020 to 2022. Results. The article analyzes the existing data of meta-analyzes regarding the incidence of cardiovascular disease, the effect of diabetes mellitus on the course of the disease, existing theories of the pathogenesis of cardiovascular disease in COVID-19. Clinical variants of heart damage in COVID-19 are also given, which are described in the literature today. Particular attention is paid to the peculiarities of development and the course of postcoid myocarditis, which is one of the most common complications of COVID-19 on the part of the cardiovascular system. Particularly noteworthy is the fact that according to the literature, some patients were diagnosed with myocarditis without clinical manifestations. Therefore, the data of histological examination of the myocardium of COVID-19 patients without myocarditis symptoms are of great interest. Difficulties also arise at the stage of diagnosing myocarditis, because the currently accepted methods are sometimes uninformative. Mass vaccination against COVID-19, unfortunately, also had such undesirable side effects as post-injection myocarditis. The literature data on the development of dilated cardiomyopathy after suffering from COVID-19 are presented. At the same time, dilated cardiomyopathy can be the result not only of the transferred myocarditis, but also a primary reaction to the effect of cytokines released by the virus. Conclusions. COVID-19 can exacerbate pre-existing cardiovascular pathology and provoke acute myocardial injury, deteriorating the course of the disease and the life prognosis for the patient.
Status and Analysis of 832 Investigator-Initiated Coronavirus-Related Clinical Studies
Authors: Zhang Yurong , Wang Li , Wang Ruizhong , Qi Zhang , Xu Rui
Published: May 2024

Objective: To contribute to the development of clinical research on novel coronavirus by analyzing the clinical research data of COVID-19. Methods: Searches were performed on the database of “National Health Insurance Information Platform Medical Research Registration Information System” using the keywords “COVID-19” and “Novel coronavirus.” The search was performed till 31 December 2022. This paper presents a statistical analysis of the status quo of the registered projects in terms of the number of registered projects, the types of projects, the levels of the institutions, the types of research, the intervention measures, the research design, the main objectives of the research, and so on. Results: A total of 823 investigator-initiated clinical studies of COVID-19 were documented, and the number of studies registered peaked on December 31, 2020, and December 31, 2022. Among them, there were 819 items from general medical research (99.5%), 812 items from medical institutions (98.7%), and 713 items from Medical Grade III, and Class A hospitals (86.6%). Among these items, 534 (64.9%) were observational studies. The most common intervention method used was administering existing drugs, with 140 studies utilizing them. This data analysis also included 128 case-control studies and 247 treatment-oriented studies. Conclusion: Researchers in local medical institutions have been actively carrying out clinical research related to COVID-19. However, they should refer to registered research to avoid duplicate research.
Research Progress of miRNA in Diabetic Nephropathy
Authors: Du Yan , Cai Yu , Tian Chang , Jia Yuanyuan , Feng Yu
Published: May 2024

Diabetic nephropathy (DN) accounts for approximately 20–40% of diabetic patients. It is one of the most common microvascular complications of diabetes and an important cause of end-stage renal disease (ESRD). Renal biopsy histopathology is an important means for early clinical diagnosis of DN, but because it is an invasive examination, it is not easily accepted by patients due to risks such as bleeding, infection, and thrombosis. Therefore, finding new biomarkers for diagnosing DN is of great significance for early treatment and improving patient prognosis. MicroRNA (miRNA) is a type of highly conserved endogenous non-coding RNA. More and more studies have shown that miRNA is involved in the pathological process of DN and renal fibrosis. This article summarizes the relevant research on miRNA in DN.
The Relationship Between Spousal Support and Pregnancy Stress in High-Risk Pregnant Women
Authors: Özbek Zeynep , Beydağ Kerime Derya
Published: May 2024

Objective: This research was conducted to establish the relationship between spousal support and pregnancy stress in high-risk pregnant women. Methods: The sample of the descriptive and correlational study consisted of 220 pregnant women who were hospitalized for treatment in the perinatology service of the Women’s and Children’s Hospital on the Anatolian side of Istanbul between 1st December 2020 and 1st February 2021. Data were collected using a descriptive data collection form, the Pregnancy Stress Assessment Scale, and the Spousal Support Scale. Data analysis involved numerical and percentage calculations, ANOVA test, Kruskal Wallis test, t-test, and Spearman correlation analysis. Results: The average score on the Pregnancy Stress Assessment Scale for the participating pregnant women was 50.24 ± 27.10, and the average score on the Spousal support scale was 57.69 ± 9.21. No statistically significant relationship was found between the average scores on the Pregnancy Stress Rating Scale and the Spousal Support Scale for pregnant women (P > 0.01). Conclusion: It is recommended that nurses and midwives working with high-risk pregnant women incorporate plans to reduce pregnancy stress into their care processes.
Investigation and Analysis of Factors Influencing the Quality of Community Nursing Services in Laishan District, Yantai City
Authors: Sun Jianjun , Ji Qing , Abonalla Arby James , De Ala Minerva B.
Published: May 2024

Objective: The purpose of this study is to conduct an in-depth investigation and analysis of the factors affecting the quality of community nursing services in Laishan District, Yantai City, and provide suggestions for improving the quality of the services. Method: The community nursing services in Laishan District, Yantai City were investigated through questionnaires and on-site observations. Result: The content of nursing services was correlated with the professional titles of the staff. The guidance given to patients and caregivers was significantly correlated with the educational level of community nursing personnel. Therefore, this indicates that the professional titles and educational backgrounds of the community nursing personnel have a significant impact on the quality of service provided. Conclusion: The role of professional title evaluation can be optimized, further enhancing the educational level of nurses in surrounding communities, optimizing service content and methods, etc., in order to improve the quality of community nursing services in the region.
Analysis of Combining OBE and CBL in Pharmacy Internship
Authors: Yang Yuqiong , Wu Yanyan , Fa Yanmei , Wu Qionglian
Published: May 2024

Talent training has been emphasized in China’s national development. Training an excellent pharmaceutical professional has far-reaching significance for promoting rational use of drugs, ensuring safe drug use, and improving the quality and safety of medical treatment. The internship program is an important part of a pharmacy major that serves to develop students’ practical skills. In this paper, we propose a combination of outcome-based education (OBE) and case-based learning (CBL) in pharmacy internships to promote critical thinking and independent learning among students and ensure the sustainable development of pharmacy education.
Study on the Impact of Cognitive Nursing Model on Postoperative Quality of Life in Patients Undergoing Chronic Sinusitis Surgery
Authors: Cao Xiaojuan
Published: May 2024

Objective: To explore effective nursing programs for surgical patients with chronic sinusitis (CS). Methods: 62 CS surgery patients were randomly divided into a research group and a control group with 31 cases in each group. The patients in the research group received cognitive nursing care, while the patients in the control group received routine nursing care. The nursing effects of the two groups were compared. Results: The postoperative quality-of-life scores, health behavior scores, complication rates, and nursing satisfaction scores of the patients in the research group were all better than those of the control group (P < 0.05). Conclusion: The cognitive nursing model significantly improves the condition of CS surgery patients, thus it should be popularized.
Study of the Effect of Preoperative Dietary Intervention on Gastrointestinal Reaction of Microparticle TACE
Authors: Liu Weifang , Lin Jingyi
Published: May 2024

Objective: To evaluate the effect of preoperative dietary intervention on the gastrointestinal reaction of microparticle transcatheter arterial chemoembolization (TACE). Methods: A total of 60 patients with primary liver cancer who were treated in our hospital from January 2018 to October 2018 were selected for this study. The random sampling method was used to divide the patients into an intervention group (30 cases) and a control group (30 cases). The control group received routine intervention, whereas the intervention group received optimized nursing intervention. The incidence of gastrointestinal reactions, the vomiting frequency, the average vomiting volume, the incidence of stomach discomfort, the quality of life, and the anxiety and depression indicators of both groups were compared. Results: The results of all indicators of the intervention group were better than those of the control group. The incidence of gastrointestinal reactions in the intervention group was 13.9%, which was lower than 43.3% of the control group. The scores of QLQ-C30 of the intervention group were higher than those of the control group; the SAS and SDS scores in the intervention group were lower than those of the control group. Conclusion: Preoperative dietary intervention can reduce the incidence of gastrointestinal reactions of microparticle TACE and improve the symptoms of gastric discomfort among patients.
Effect of Resistance Training, Aerobic Exercise Before Doula-Assisted Delivery on the Psychological Well-Being and Labor Duration of Parturients
Authors: Zhou Jing , Tang Sha , Zhou Li , Jiang Cong , Fan Wenjian
Published: May 2024

Objective: To investigate the impact of resistance training and aerobic exercise before doula-assisted delivery on the psychological well-being and labor duration of parturients. Methods: 105 cases of parturients who gave birth in our hospital from February 2021 to April 2023 were included in this study. The patients were divided into a control group of 52 cases and an observation group of 53 cases. The patients in the control group received no particular intervention before delivery and only received doula assistance. The patients in the observation group received resistance training, aerobic exercise, and doula assistance prior to giving birth. After delivery, the mothers were observed for 2 hours in the delivery room and then returned to the maternal and infant ward. The observation indicators of the two groups were compared. Results: In comparison to the control group, the observation group exhibited shorter durations in the first, second, and third stages of labor, as well as the total delivery time. Additionally, the amount of postpartum blood loss at 2 hours was smaller in the observation group. The rate of cesarean section was lower, and the rate of vaginal natural delivery was higher in the observation group. Following the intervention, both groups of postpartum women showed decreased SAS and SDS scores compared to before the intervention. Moreover, the observation group had lower scores than the control group. These differences were all statistically significant (P < 0.05). Conclusion: Resistance training and aerobic exercise before doula-assisted delivery can effectively improve the psychological state of parturients, shorten labor duration, increase the rate of natural vaginal delivery, and reduce pain and postpartum hemorrhage.
Application Value of Evidence-Based Nursing in the Perioperative Care of Ankle Fracture Patients
Authors: Wang Lin
Published: May 2024

Objective: To analyze the value of applying evidence-based nursing in the perioperative care of patients with ankle fractures and propose corresponding measures to improve the quality of patient care. Methods: The study started in May 2022 and was terminated in March 2023. During this period, 50 patients with ankle fractures treated by our hospital were selected as research subjects. They were divided into a control group and a research group. The control group had 25 patients who received routine care, and the research group had 25 who received evidence-based care. The nursing effects of the two groups were observed and compared. Results: The pain scores, quality of life, incidence of adverse reactions, and nursing satisfaction of patients in the research group were all significantly better than those in the control group (P < 0.05). Conclusion: Evidence-based nursing can effectively improve the quality of care, reduce the risk of adverse reactions, and promote patient recovery, so it is suitable for clinical implementation.
Analysis of the Correlation Between Visceral Fat Area and Insulin Resistance in Patients with Type 2 Diabetes and Abdominal Obesity
Authors: Zhang Guohui , Xu Juan , Peng Qiong , Xu Yalei , Ma Shaochang
Published: May 2024

Objective: To analyze the correlation between visceral fat area and insulin resistance index (HOMA-IR) in patients with type 2 diabetes mellitus (T2DM) and abdominal obesity and to provide a reference for screening and related research of such patients. Methods: Two hundred patients with T2DM admitted to Guandu People’s Hospital of Kunming were included. The study was carried out from October 2022 to December 2023. The patients were divided into three groups according to different abdominal visceral fat areas (VFA): Group A (n = 65) was less than 75cm2, Group B (n = 75) was 75–100 cm2, and Group C (n = 60) was greater than 100 cm2. The subjects in the three groups were all tested for glycated hemoglobin (HbA1c), fasting insulin (FINS), and fasting blood glucose (FPG). Height and weight were measured to calculate body mass index (BMI). The HOMA-IR and TYG (fasting triglyceride and glycemic index) were also calculated. Changes in the BMI, VFA, HOMA-IR, and TYG levels were observed in the three groups. Results: The VFA, BMI, HbA1c, FPG, FINS, HOMA-IR, and TYG of the patients all increased, with a more significant increase in the BMI, FINS, HOMA-IR, and TYG levels (P < 0.01). Multiple linear stepwise regression analyses used visceral fat area (VFA) as the dependent variable. The results showed that VFA was closely related to BMI, FINS, HOMA-IR, and TYG. Conclusion: Early reduction of VFA to reduce insulin resistance may be a better treatment and effective method for T2DM, providing powerful measures and new strategies for effective blood sugar control and early prevention in the treatment of metabolic diseases.
Ján Jiskra z Brandýsa a vdovské majetky Alžbety Luxemburskej v Hornom Uhorsku (banské mestá a ich úloha vo financovaní vojenských aktivít)
Authors: Maliniak Pavol
Published: May 2024

Pôsobenie Jána Jiskru v Uhorsku doterajšie bádanie skúmalo najmä na pozadí politických a vojenských dejín. Výskumy sa iba okrajovo venovali aktivitám Jiskru a jeho strany v oblasti hospodárstva. Kontakty Jiskru so zväzom siedmich banských miest v rokoch 1442–1457 tvoria sondu do širšej problematiky. Približujú konkrétne formy ekonomickej spolupráce – financovanie vojska, hradov a obrany v závislosti od meniaceho sa rozloženia moci. Ústrednú úlohu v nich zohrávala kontrola Jiskru nad banskou a mincovou komorou v Kremnici, ktorá zároveň napomáha lepšie porozumieť jeho kompetenciám.