bluuebunny/crossref_metadata_2025 huggingface dataset:
Polyunsaturated Fatty Acids Promote Protumoral Macrophage Polarization via a RhoA-YAP1 Signaling Pathway in the Ovarian Cancer Microenvironment
Authors: Chan David , WANG Huogang , Yung Mingo , Xuan Yang , Chen Fushun , Chan Wai-Sun , Siu Michelle , LONG Runying , LIANG Yonghao , Xu Dakang , Tsui Stephen , Ngan Hextan , Chan Karen
Published: December 2022

Tumor-associated macrophages (TAMs) are crucially associated with tumor development and progression; however, it remains unclear how the tumor microenvironment (TME) rewires the metabolic circuits and preferentially induces TAMs to polarize toward a protumoral phenotype. Here, we report that polyunsaturated fatty acids (PUFAs) in malignant ascites promote protumoral M2-like TAMs deposition and facilitate peritoneal metastases of epithelial ovarian cancer (EOC). We demonstrated that PUFAs in the lipid-enriched malignant ascites inactivate RhoA, reducing nuclear YAP1 in macrophages and promoting protumoral M2-like TAMs polarization with OXPHOS metabolism. Conditional Yap1 depletion in murine MΦs leads to skew macrophage polarization toward protumoral M2-like TAMs that, in turn, suppress CD8+ T cell infiltration and aggravate tumor colonization in vivo. Noticeably, the significance of nuclear YAP1 depletion was evinced in the infiltrating TAMs in tumor spheroids of malignant ascites from EOC patients. In contrast, restored nuclear YAP1 expression in TAMs by pharmacological suppression of MST1/2 enhances tumoricidal M1-like TAMs population and CD8+ T cells infiltration, restricting EOC peritoneal metastasis. These results indicate that PUFAs are a key player in promoting tumor-infiltrated TAMs polarization that, in turn, facilitates EOC tumor growth and metastasis.
The role and power of visual trigger on the experience of Autonomous Sensory Meridian Response (ASMR)
Authors: Maeno Ami , Kajimura Shogo
Published: December 2022

Despite the growing research interest in the autonomous sensory meridian response (ASMR), research on the triggers that induce ASMR has been scarce. In particular, the role of visual triggers in ASMR induction remains largely unknown. The current study, using the newly developed stimulus set, showed that the enhancement of auditory-induced ASMR by simultaneously presented visual triggers is due to information about the source of auditory triggers and that ASMR can be induced by visual triggers alone. In Experiment 1, we assessed whether the congruency of auditory and visual triggers affected the experience of ASMR and found that the occurrence of ASMR was significantly more frequent in the condition in which these triggers were congruent than when they were incongruent. In Experiment 2, we assessed whether the visual trigger was capable of inducing ASMR by itself and found that, although the frequency of ASMR occurrence was lower in the visual trigger than the auditory trigger only, the visual trigger was single-handedly able to induce ASMR. The generalization of these findings is provided by the sufficient number of newly prepared stimuli that successfully induced ASMR within a short duration. These results may facilitate psychophysiological research on the properties of ASMR-inducing stimuli.
Association of Deepression and Anxiety With Uncontrolled Hypertension: a Cross-sectional Study in Southwest Nigeria
Authors: Amaike Chikwendu , Salami Omotayo Felicia , Bamidele Olabisi Titilayo , Ojo Abayomi Moses , Otaigbe Idemudia , Abiodun Olumide , Olumide Adesola , Adebiyi Akindele Olupelumi
Published: December 2022

Background
Hypertension is a medical condition of public health concern that increases the risk for chronic non-communicable diseases and mortalities. In recent years, understanding its co-existence with other comorbidities have been the focus of better management. However, the relationship between hypertension and depression or anxiety has been contentious with diverse findings reported by different studies. Understanding the relationship between depressive and anxiety disorders and hypertension control will be crucial for reducing the mortality associated with hypertension. Therefore, this study was conducted to determine the association between depression or anxiety and hypertension control.
Methods
A cross-sectional study was conducted among 321 hypertensive patients accessing care in two hospitals. Depression and anxiety symptoms were assessed using the Hospital Anxiety and Depression Scale. Relationship between uncontrolled hypertension and depression or anxiety was assessed using binary logistics regression.
Results
The prevalence of depression and anxiety was 12.1% and 23.1% respectively. Total of 60 and 261 of the participants had controlled and uncontrolled blood pressure respectively. Out of the 261 participants with uncontrolled blood pressure, 14.2% and 23.4% had depression and anxiety respectively. Depression (OR=7.751, 95% CI=1.79-43.4, P=0.011) was associated with an increased risk for uncontrolled blood pressure after adjusting for sex, smoking of cigarette, age, marital status, and exercise.
Conclusion
There is a statistically significant relationship between depression and uncontrolled blood pressure. It is therefore relevant for healthcare providers to assess for depression in patients with hypertension and provide treatment to achieve hypertension control.
Different impacts of TP53 mutations on cell cycle-related gene expression among cancer types
Authors: Sasaki Keiju , Takahashi Shin , Ouchi Kota , Otsuki Yasufumi , Wakayama Shonosuke , Ishioka Chikashi
Published: December 2022

Functional properties caused by TP53 mutations are involved in cancer development and progression. Although most of the mutations lose normal p53 functions, some of them, gain-of-function (GOF) mutations, exhibiting novel oncogenic functions. No reports have analyzed the impact of TP53 mutations on the gene expression profile of the p53 signaling pathway across cancer types. This study is a cross-cancer type analysis of the effects of TP53 mutations on gene expression. A hierarchical cluster analysis of the expression profile of the p53 signaling pathway classified 21 cancer types into two clusters (A1 and A2). Changes in the expression of cell cycle-related genes and MKI67 by TP53 mutations were greater in cluster A1 than in cluster A2. There was no distinct difference in the effects between GOF and non-GOF mutations on the gene expression profile of the p53 signaling pathway.
Regional variations and sex-related differences in human tracheal ligaments
Authors: Fukushige Kaori , Okubo Tomohito , Shan Xiyao , Takeuchi Takao , Misaki Noriyuki , Naito Munekazu
Published: December 2022

This study aimed to investigate the characteristics of the ligaments present between the thyroid, cricoid, and tracheal cartilages on living human bodies and cadavers using a digital palpation device. The mean dynamic stiffness values of the cricothyroid ligaments were 622.0 ± 88.2 N/m (mean ± SD) for humans, 956.1 ± 75.0 N/m when measured directly in the cadavers, and 826.5 ± 105.7 N/m when removed from the cadavers. There was no significant difference in dynamic stiffness between the cricothyroid and the first annular ligaments. The dynamic stiffness of annular ligaments measured in the superior trachea of human subjects and cadavers decreased gradually toward the distal trachea, whereas the mechanical stress relaxation time increased distally. There was no difference in dynamic stiffness between males and females in the upper part of the trachea; however, the dynamic stiffness of the middle and inferior parts was significantly higher in females than in males. Furthermore, males, but not females, showed significant differences in dynamic stiffness between the superior and middle parts. These results reveal that there are regional and sex-related differences in tracheal ligaments. This information might prove useful for surgical airway management and so on.
Radiomics prediction of the pathological grade of bladder cancer based on multi-phase CT images
Authors: Jing Qian , Yang Ling , Hu Su , Gu Siqian , Yao Feirong , Hu Chunhong , Yao Tianyang , Dai Sunxian , Shen Ying
Published: December 2022

Background
The pathological grade of bladder cancer(BCa)is a critical determinant for the follow-up clinical decision and treatment of patients. The authors investigated a radiomic-clinical model in predicting the pathological grade of BCa.
Objective
This study explored the feasibility of the radiomics based on multi-phase thick-slice CT images combined with clinical risk factors in predicting of the pathological grade of BCa.
Methods
Patients with BCa who underwent CT scan and surgical treatment from January 2019 to December 2021 were analyzed retrospectively, with 104 cases of high-grade BCa and 100 cases of low-grade BCa included. Radiomics features were extracted from tumor volume in the images of the plain scan, corticomedullary phase, and parenchymal phase, respectively. Logistic Regression model, SVM model, and Random Forest model were established, and the model with higher diagnostic efficiency was chosen. Additionally, a radiomics-clinical model was conducted by selected independent predictors according to logistic regression analysis. Then the performance of the model was assessed.
Results
Among the 204 patients enrolled, the training cohort was consisted of 142 patients and the validation cohort was made up of 62 patients. The Logistic Regression model proved to be the most effective one among the three models. The radiomics-clinical model consisted of 2 independent predictors, patient age and Rad-Score, with an AUC of 0.904(95%CI 0.857–0.951) and 0.906༈95%CI 0.837–0.975༉in the training and validation cohorts, respectively. The diagnostic accuracy, sensitivity, and specificity of the validation cohort were 0.790, 0.813, and 0.767 respectively.
Conclusion
The radiomics-clinical model possesses great potential in predicting the pathological grade of BCa.
The bleaching effect of office bleaching agents containing S-PRG filler
Authors: Akabane Kodai , Hiraishi Noriko , Shimojima Mika , Nassar Mohannad , QI Feng , Otsuki Masayuki , Shimada Yasushi
Published: December 2022

Objectives:Surface reaction-type pre-reacted glass-ionomer (S-PRG) filler releases six types of ions with a neutralizing ability. This study evaluated the effect of S-PRG filler incorporation in an H2O2-based bleaching material on the bleaching efficacy, pH, and reaction state.Materials and Methods:The experimental bleaching material was formulated by the addition of 5% or 10% S-PRG fillers to the powder part. The stained bovine teeth were treated with the prepared bleaching paste. Commission internationale de l'éclairage (CIE) L*a*b* color space values were recorded before and after bleaching, and the color difference (ΔE) was calculated. Moreover, the used bleaching formulations were assessed for their pH values and reaction state by evaluating the oxidation state of manganese (Mn+2) using electron spin resonance (ESR).Results:Bleaching efficacy was increased by addition of S-PRG filles; no significant difference was observed between groups containing 5% and 10% S-PRG fillers. A significant increase in pH in S-PRG filler groups (5% pH 6.7, 10% pH 6.8) was found compared to the 0 % group (pH 4.8). ESR measurements showed that the signal emitted from Mn+2decreased over time. The S-PRG filler groups showed a significantly higher reduction in Mn+2compared to the 0% group, with no significant difference between the 5% and 10% S-PRG groups.Conclusions:S-PRG filler addition resulted in improved bleaching efficacy, higher reaction rate, and pH values that are close to neutral.Clinical Relevance:The effect of S-PRG filler addition may be effective on bleaching of H2O2-based martials.
The association between intimate partner violence and cervical cancer screening among women of childbearing age: A South African Case Study
Authors: Hollington Marcus
Published: December 2022

Background
In South Africa, cervical cancer is the second most prevalent cancer. Amidst the disease’s prevalence is the presence of Intimate Partner Violence in South Africa, which could impair the ability of women to undergo cervical cancer screening. Thus, illustrating its negative implications on health-seeking behavior. This study examined if, and how intimate partner violence among women aged between 15 and 49 years is associated with cervical cancer screening.
Methods
The study used cross-sectional data from the South Africa Demographic and Health Survey conducted in 2016. The population of interest was women aged between 15 and 49 years that underwent cervical cancer screening. Bivariate analysis was conducted to investigate the relationship between cervical cancer screening and each of the study’s independent variables. Finally, a binary regression model was used to determine the association between intimate partner violence and cervical cancer screening among women aged between 15 and 49 years, whilst controlling for other variables.
Results
The study found that 39% of women aged between 15 and 49 years underwent cervical cancer screening in South Africa at least once in their lifetime. Women that experienced intimate partner violence were more likely to undergo cervical cancer screening compared to women that did not experience intimate partner violence (UOR: 1.33; p < 0.05; CI: 1.07–1.65 and AOR: 1.46; p < 0.05; CI: 1.14–1.86). Area of residence, age, educational attainment, province, wealth quantile, ethnicity, number of sex partners, frequency of cigarette smoking, contraception, and health insurance were also found to be statistically associated with cervical cancer screening.
Conclusion
The study contributed to our understanding of predictors of cervical cancer screening in South Africa, particularly the association between intimate partner violence and cervical cancer screening. It found that women who experience intimate partner violence are more likely to undergo cervical cancer screening compared to women that do not experience intimate partner violence. This is because women who experience intimate partner violence are at greater risk of contracting cervical cancer. Thus, their need to undergo cervical cancer screening. This highlights the severity of intimate partner violence in South Africa and the health risks it poses to women, particularly regarding cervical cancer.
Impact of thyroid hormone treatment on maternal pregnancy outcomes in women with subclinical hypothyroidism without TPOAb
Authors: Sitoris Georgiana , Veltri Flora , Jelloul Emna , Kleynen Pierre , Rozenberg Serge , Poppe Kris G
Published: December 2022

Purpose: To investigate the impact of thyroid hormone treatment (LT4) on maternal pregnancy outcomes in women with subclinical hypothyroidism (SCH) without thyroid peroxidase antibodies (TPOAb) positivity.
Design/Methods: Single centre, cross-sectional study in 1460 women screened for TSH, free T4 and TPOAb at median 13 (11-17) weeks of gestation during the period 2013-2014. Exclusion criteria were twin- and assisted reproduction pregnancies, TPO positivity, overt hyperthyroidism, and treatment with LT4 before screening. The impact of LT4 on maternal pregnancy outcomes was investigated in a group of 53 women with SCH (TSH >3.74 mIU/L) in which LT4 was initiated at median 13 (10-22) weeks (treated group). The control group included 18 women with SCH (TSH >3.74 mIU/L; untreated group). The prevalence of pregnancy complications in these two groups was compared with that in a reference group of 1389 women (TSH £3.74 mIU/L and no TPOAb; REF group).
Results: The prevalence of pre-eclampsia and gestational diabetes (GDM) was higher in the control group vs that in the REF group (16.7% vs 5.0% and 27.8% vs 18.9%; p=0.017 and p=0.016, respectively), but comparable in the LT4 treated SCH group vs the REF group (7.6% vs 5.0% and 22.6% vs 18.9%; p=0.918 and 0.676, respectively). The prevalence of iron-deficiency anaemia was lower in the LT4 treated vs the REF group (17.0% vs 32.5%; p=0.017).
Conclusions: Pregnant women with SCH and without TPOAb positivity who were LT4 untreated had a higher prevalence of pre-eclampsia and GDM compared with euthyroid women, while this was not the case when SCH women were treated with LT4, even when it was initiated after the first trimester.
Simulation of a plausible great earthquake (Mw 8.0) sourced on down-going Indian slab beneath Indo-Burmese wedge and its implications for North East (NE) Indian region
Authors: Chingtham Prasanta , Sharma Babita , Mohan Kapil , Mittal Himanshu
Published: December 2022

The 4th January 2016 Manipur earthquake (Mw 6.7) occurred along the Indo-Burmese wedge and ruptured towards NW direction causing severe damage to buildings/structures in North-East (NE) Indian region. A plausible earthquake (Mw 8.0) is simulated to estimate the ground motions and associated seismic hazard by means of the waveforms of 2016 Manipur earthquake (Mw 6.7) as an element earthquake considering the source in the subduction boundary of the Indo-Burmese wedge at an intermediate depth. The empirical greens function mechanism (EGFM) is adopted to accomplish the better utilization of the observed ground motions of the recorded earthquake as an element earthquake and to achieve the probable ground motions in order to acquire the appropriate path/site effects in the simulated ground motions. The obtained results demonstrate the impact of the comparable rupture directivity pattern in both element as well as the simulated earthquakes. The Peak Ground Acceleration (PGA) in NE India from element and simulated earthquakes vary from 3 cm/sec2 and 11 cm/sec2 to 103 cm/sec2 and 342 cm/sec2 at epicentral distances of 624 km and 53 km respectively. The high amplitude surface waves due to the interference of seismic waves along with the combined effects of rupture directivity and site amplification showcased the highest PGA value at Shillong (SHL). This site is located on Pre-Cambrian rock and situated at an epicentral distance of 214 km from the source zone, which is lying at an intermediate depth that might have propelled the direct seismic waves of higher intensity at a longer distance compared to other sites. The outcome of the present study highlights the significance of varied ground motion parameters among the observed sites to the extent of bearing the damage potential of strong ground motions. The related analysis also advocates for the simulated PGA variations and associated duration of the earthquake waveforms exposed on different geological formations that have strong bearings on the seismic risk involved with future probable great earthquake in the study region. Moreover, simulated ground motions of expected plausible disastrous earthquakes on numerous geological formations beneath the various sites have significant impacts on designing critical structures/buildings such as schools, hospitals, bridges, dams and nuclear power plants for NE India. Thus, the detailed investigations on ground motion parameters, simulation of ground motion and the influence of different geological/geomorphological conditions on duration, shape and maximum amplitude of ground motion may be supportive for implementing earthquake risk and mitigation plans in order to assess the seismic hazard of the study region.
Clinical Prediction Model for Severe Pain After Percutaneous Nephrolithotomy and Analysis of Associated Factors ：A Retrospective Study
Authors: Wei Yuzhi , Wu Haotian , Qi Ziheng , Feng Chunyu , Yang Bo , Yin Haolin , Wang Lu , Zhang Huan
Published: December 2022

Objectives:This study aimed to develop severe pain prediction models for patients after percutaneous nephrolithotomy (PCNL), to identify and intervene the risk factors causing pain in a timely manner so as to improve patient satisfaction after surgery.Methods:The clinical data of 331 PCNL patients were retrospectively analyzed. Data of 265 patients (80%) were randomly selected as the training cohort and those of 66 (20%) patients were selected as the validation cohort. We choosed clinical data through the stepwise Akaike information criterion and best subset selection to build severe pain prediction models by the training data set. The validity of the model was verified and analyzed using the test data set.Results:The first clinical model was developed using the American Society of Anesthesiologists (ASA) classification, number of kidney stones, history of hydronephrosis, duration of surgery, and presence of stone remnants after surgery. This model had an area under the curve (AUC) of 0.8682 (95% confidence interval [CI], 0.8205–0.9160), threshold of 0.4771, sensitivity of 97.25%, specificity of 63.86%, and negative predictive value (NPV) of 91.38%. The second model was developed using the mentioned above six clinical features and size of kidney stones. This model showed a stronger discriminatory power (P=0.208), with an AUC of 0.8710 (95% CI, 0.8242–0.9187), threshold of 0.4813, sensitivity of 97.25%, specificity of 83.86%, and NPV of 91.38%. In the subsequent validation cohort (N = 66), the AUC (95% CI) was 0.9093 (0.8378–0.9808) for the first clinical model and 0.9098 (0.8373–0.9823) for the second clinical models.Conclusion:We developed two prediction models for postoperative pain severity in PCNL patients, which were validated internally.Trial registration:This is a retrospective study that does not include sensitive patient data, but only data provided by professionals from selected centres about their daily clinical practice. Therefore, our ethics Committee did not consider it necessary to register the study.
Hierarchical V4C3TX@NiO-reduced graphene oxide heterostructure hydrogel and defective reduced graphene oxide hydrogel as free-standing anode and cathode for high-performance asymmetric supercapacitor
Authors: Tian Yuqin , Chen Weiwen , Qiu Zenghui , Jing Jian , Xu Haijun , Pan Dingjie , Chen Shaowei
Published: December 2022

Asymmetric supercapacitors (ASCs) based on a battery-type anode and a capacitive-type cathode have been attracting extensive interest because of their high energy density. Herein, NiO nanosheets are hydrothermally deposited onto a V4C3TXsubstrate, which are then assembled into a 3D porous heterostructure hydrogel through a graphene oxide-assisted self-convergence hydrothermal process at low temperatures. The resultant hierarchical V4C3TX@NiO-reduced graphene oxide (RGO) hydrogel exhibits an ultrahigh specific capacitance up to 1014.5 F g− 1at 1 A g− 1. Separately, a defective reduced graphene oxide (DRGO) hydrogel is prepared by a cost-effective hydrothermal procedure followed by cobalt-catalyzed gasification, which shows a higher specific capacitance (258 F g− 1at 1 A g− 1) than untreated RGO hydrogel (176 F g− 1). These two electrodes are then assembled into an ASC, the device features a stable operating voltage of 1.8 V, a maximum energy density of 86.22 Wh kg− 1at 900 W kg− 1, along with good cycling stability at 96.4% capacitance retention after 10,000 cycles at 10 A g− 1. This work highlights the unique potential of MXenes-based materials for the construction of high-performance ASCs.
A Computed tomography (CT) based morphometric study of superior pubic ramus anatomy among Arabs to determine safe intramedullary pubic rami screw insertion
Authors: Jarragh Ali , Lari Ali , Sheikh Mehraj
Published: December 2022

Purpose
To measure the morphometric variables of the superior pubic ramus in a sub-set of an Arab population to establish a safe pubic screw fixation technique.
Methods
Cross sectional retrospective analysis of computed tomography (CT) pelvis images for 231 participants. The morphometric analysis included; pubic ramus axis length, insertion angles orientation, distance from the exit point to the pubic symphysis, distance from the entry point to acetabular joint surface and assessment of the narrowest pubic ramus diameter at three anatomical zones: para-symphyseal, mid-pubic and supra-acetabular areas. Pubic rami diameter correlation with age and sex was also analyzed.
Results
A total of 231 participants were included (55% male, 45% female). The mean screw length was between (104–127 mm) and it is significantly higher in males than females. The narrowest canal diameter was found at the para symphyseal area in both sexes were; 7.35mm in males and 4.75 mm in females. In females, all canal diameters at the three measured regions were significantly smaller than male’s diameters. The mediolateral insertion angle was significantly higher in females than males (49.4° vs. 41.8°, respectively), whereas the cephalic-caudal angle was significantly higher in males than females (49.9° vs. 42.1° respectively). The mean distance from the lateral ilium entry point to joint articular surface was higher in males (23.5 mm) than females (19.9 mm).The symphysis pubis to tubercle exit point was significantly higher in females than males (24.2 mm vs 16.6 mm respectively). Pearson’s correlation analysis revealed a statistically significant positive correlation between age and the pubic ramus diameter at the three measured regions in all age groups.
Conclusion
The results from this study suggest that percutaneous pubic rami screw fixation using the standard 6.5 or 7.3mm cannulated screw system may potentially be unsafe in female Arab patients. Thus, a closer evaluation in this subset of patients may require the alternative solid non-cannulated screws (3.5- 4.5mm) or plate fixation options. Further, female patients may have a higher risk of acetabular joint penetration, while males have a potentially higher risk of pudendal nerve injury
Metagenomic and culture-dependent approaches unveil active microbial community and novel functional genes involved in arsenic mobilization and detoxification in groundwater
Authors: Diba Farzana , Hoque M. Nazmul , Rahman M. Shaminur , Rahman Md. Khandakar Jaminur , Moniruzzaman Md. , Khan Mala , Hossain M. Anwar , Sultana Munawa
Published: December 2022

Background: Arsenic (As) and its species are major pollutants in ecological bodied including groundwater in Bangladesh rendering serious public health concern. Bacteria with arsenotrophic genes have been found in the aquifer, converting toxic arsenite [As (III)] to less toxic arsenate [As (V)] that is easily removed using chemical and biological trappers. In this study, genomic and metagenomic approaches parallel to culture-based assay (Graphical abstract) have made it possible to decipher phylogenetic diversity of groundwater arsenotrophic microbiomes along with elucidation of their genetic determinants.
Results: Seventy-two isolates were retrieved from six As-contaminated (average As concentration of 0.23 mg/L) groundwater samples from Munshiganj and Chandpur districts of Bangladesh. Twenty-three isolates harbored arsenite efflux pump (arsB) gene with high abundance, and ten isolates possessing arsenite oxidase (aioA) gene, with a wide range of minimum inhibitory concentration, MICAs (2 to 32 mM), confirming their role in arsenite metabolism. Shotgun metagenomic analysis revealed considerable parallels with the culture-dependent method proving their complementarity in detecting native population bacteria in As containing aquifers. There was considerable heterogeneity in species richness and microbial community structure. Microbial taxa from Proteobacteria, Firmicutes and Acidobacteria dominated these diversities. Through these combinatorial approaches, we have identified potential candidates such as, Pseudomonas, Acinetobacter, Stenotrophomonas, Achromobacter, Paraburkholderia, Comamonas and Klebsiella and associated functional genes (arsB, acr3, arsD, arsH, arsR) that could significantly contribute to arsenite detoxification, accumulation, and immobilization.
Conclusions: Culture-dependent and -independent shotgun metagenomic investigation elucidated arsenotrophic microbiomes and their functions in As biogeochemical transformation. These findings provide scientific basis for mitigating arsenic pollution in the groundwater of Bangladesh and beyond.
Correlation between Serum Heat Shock Protein 27 and Interleukin- 17 Levels and Prognosis in Patients with Acute Myocardial Infarction
Authors: Hu Ende , Ke Jiaxing , Lu Nan , Lin Xinmiao , Chen Sen , Chen Haifeng
Published: December 2022

Background
Heat shock protein 27 (HSP27) and interleukin-17 (IL-17) are important indicators of inflammation. Whether serum HSP27 and IL-17 levels hold predictive value on the prognosis of patients with acute myocardial infarction (AMI) remains unclear.
Methods
This was a prospective cohort study enrolled 140 patients in the Department of Cardiology of Fujian Provincial Hospital from December 2020 to June 2021, including 40 patients of non-AMI and 100 patients of AMI. The levels of serum HSP27, Phosphorylated HPS27 (pHSP27) and IL-17 were measured by enzyme linked immunosorbent assay. Patients with AMI were followed up. The follow-up endpoint was major adverse cardiovascular events (MACEs), including recurrent angina pectoris, re-admission heart failure, re-admission myocardial infarction, stroke, and death. And follow-up ended on December 31, 2021.
Results
HSP27, pHSP27 and IL-17 were higher in AMI patients than in non-AMI patients (All P < 0.05). HSP27 level was negatively correlated with troponin I level (r = -0.243, P < 0.05). And there were negative correlations between HSP27, IL-17 and Gensini scores, respectively (r(HSP27)=-0.374, P = 0.002;r(IL−17) = -0.289, P = 0.016). All 100 patients with AMI were followed up. The median follow-up was 8 months. There were 30 cases of MACEs. The area under the receiver operating characteristic curve for the predict of MACEs was higher for joint model (combining HSP27, pHSP27 and IL-17) than for HSP27, pHSP27 and IL-17 alone. Univariate COX regression analysis showed that HSP27 (HR = 0.968, P = 0.01), pHSP27 (HR = 0.972, P = 0.039) and IL-17 (HR = 0.979, P = 0.004) were protective factors for endpoint free survival. HSP27 and IL-17 were associated with MACEs after correction for confounding factors (HR(HSP27) = 0.972, P = 0.39; HR(IL−17) = 0.979, P = 0.004).
Conclusion
HSP27, pHSP27 and IL-17 were higher in AMI patients than in non-AMI patients. But HSP27, pHSP27 and IL-17 could reduce the occurrence of MACEs in AMI patients.
Increased expression of inactive rhomboid protein 2 in circulating monocytes after acute myocardial infarction
Authors: Dijck Phillip , Hannemann Carmen , Dreger Henryk , Stangl Verena , Stangl Karl , Ludwig Antje , Hewing Bernd
Published: December 2022

Purpose
Tumor necrosis factor-alpha (TNF-α) blood levels increase following acute myocardial infarction (AMI); TNF-α is involved in impaired recovery of myocardial function following AMI. The interaction of inactive rhomboid protein 2 (iRhom2) with TNF-α converting enzyme (TACE) is required for shedding of TNF-α from the cell surface of immune cells. In this pilot study, we hypothesized that iRhom2 expression increases in circulating monocytes following AMI.
Methods
Circulating monocytes were MACS-sorted from peripheral blood of 50 AMI patients at admission (day 1) and 3 days after admission. mRNA was isolated from sorted monocytes and expression levels of iRhom2, TACE and TNF-α were evaluated by real-time RT-PCR. Serum TNF-α levels were determined. Circulating monocyte subsets were quantified by flow cytometry. Left ventricular (LV) function was measured by echocardiography.
Results
We observed a significant increase of iRhom2 mRNA expression in monocytes (p = 0.012), of intermediate monocytes levels (p < 0.001), and of serum TNF-α levels (p < 0.001) at day 3 following AMI compared to day 1. In contrast, TNF-α and TACE mRNA expression in monocytes remained unchanged. At day 3, iRhom2 mRNA expression in monocytes positively correlated with levels of intermediate monocytes (r = 0.37, p = 0.009) and serum TNF-α levels (r = 0.33, p = 0.019). iRhom2 mRNA expression in monocytes at day 3 negatively correlated with LV systolic function (r=-0.34, p = 0.025).
Conclusions
This study suggests that iRhom2 contributes to the regulation of inflammation and is thereby associated with LV dysfunction following AMI. Thus, iRhom2 modulation should be further evaluated as a potential therapeutic strategy to attenuate adverse cardiac remodeling in AMI patients.
Faecalibacterium prausnitzii Reduces Plasma Lipopolysaccharide and Inhibits Atherosclerosis
Authors: Yang Hai-Tao , Liu Jing-Kun , Wu Ting-Ting , Yang Yi , Wu Cheng-Xin , Hou Xian-Geng , Zheng Ying-Ying , Ma Yi-Tong , Xie Xiang
Published: December 2022

Background
The gut microbiota is related to the occurrence and development of coronary artery disease (CAD). However, the specific target of gut microbiota in preventing CAD is rarely reported. The aim of this study was to identify important specific targets through metagenomics and untargeted metabolomics and to verify their relationships with atherosclerosis.
Methods
A total of 371 participants with CAD of varying severity and controls without CAD were recruited. Then, metagenomic sequencing and untargeted metabolite sequencing were used to search for important markers in faecal samples. Mice predisposed to atherosclerosis were then used to investigate the underlying mechanisms of the relationship between these species and atherosclerosis.
Results
The results of faecal metagenomics sequencing and untargeted metabolite sequencing in the CAD group and control group showed that the population with a high abundance of Faecalibacterium prausnitzii(F. prausnitzii) had the lowest incidence of CAD, and random forest importance analysis also revealed the relationship between F. prausnitzii and CAD. Moreover, we also found that F. prausnitzii was equally effective in predicting CAD alone. F. prausnitzii reduced the formation of atherosclerotic lesions in ApoE−/− mice by gavage. The mechanism involves reduction of the synthesis level of intestinal LPS and improvement of intestinal permeability by strengthening the mechanical barrier and mucosal barrier to reduce the level of plasma LPS, leading to systemic inflammation and local inflammatory reactions in the aorta, thus achieving an anti-atherosclerotic effect.
Conclusions
Sequencing of the samples identified a previously unknown link between specific gut microbiota and atherosclerosis. Treatment with F. prausnitzii may help prevent CAD.
The Impact of Negative and Positive Affectivity on the Relationship Between Work-Related Psychological Factors and Work Engagement in Japanese Workers: A Comparison of Psychological Distress
Authors: Fukuzaki Toshiki , Iwata Noboru
Published: December 2022

Background: A previous study has shown that Japanese individuals generally exhibit behavior that suppresses the expression of positive emotions, which are strongly affected by personality traits. The current study aimed: (a) to determine the extent to which negative or positive affectivity is related to work engagement (WE) and psychological distress among Japanese workers. (b) To determine the extent to which negative and positive affectivity influence the relationship between work-related psychosocial factors and WE and psychological distress.
Methods: A total of 1,000 full-time Japanese regular workers responded to an online survey that measured demographic variables, negative and positive affectivity, job demands and resources, WE, and psychological distress. A hierarchical multiple regression analysis was conducted separately, which used WE and psychological distress as dependent variables.
Results: The proportion of variance explained by negative and positive affectivity was lower for WE than for psychological distress. However, the proportion of variance defined by job demands and resources was higher for WE than for psychological distress. The interaction was only significant for the relationship between negative affectivity and job resources on psychological distress.
Conclusion: The results of this study emphasize the need to focus preferentially work environmental factors to improve WE among Japanese workers. However, workers’ WE would enhance by focusing on individual factors such as affective traits.
Double-tract vs gastric tube reconstruction after proximal gastrectomy in upper third gastric cancer: a Propensity Score Matching Analysis
Authors: Lai Chuilin , Gu Qiou , Zhan Tian , Guan Xiao , Lu Na , Jia Heng , Zhang Jianping
Published: December 2022

Background
With the popularity of function-preserving gastrectomy(FPG) and minimally invasive surgery, proximal gastrectomy(PG) has been widely used in the upper third gastric cancer. There are many reconstruction methods after PG, but the optimal one is still uncertain. The aim of this study is to compare the short-term and long-term outcomes of double-tract reconstruction(DT) and gastric tube reconstruction(Tube) after proximal gastrectomy.
Methods
This study retrospectively reviewed patients who underwent gastrectomy from January 2010 to January 2022 in our hospital. According to the reconstruction method after PG, patients were divided into DT group and Tube group. After adjusting for propensity score matching analysis, we compared the surgical outcomes, complications, and postoperative long-term reflux esophagitis and nutritional status between the two groups.
Result
After propensity score matching had been done, a total of 44 patients were included in this analysis (22 patients in the Tube and DT groups respectively). There were no significance between the two groups in postoperative complications, days of postoperative hospital stay, hemoglobin and albumin decreasing rate at 6 months, and weight loss at 6 and 12 months. The operation time of Tube group was shorter (230 vs 290 min, p = 0.005). The visick score (p = 0.012) and the rate of endoscopic reflux esophagitis (p = 0.001) of DT group were significantly lower .
Conclusion
There is no significance in postoperative complications and nutritional status between double-tract and gastric tube reconstruction after PG. Tube reconstruction can greatly reduce the operation time, while double-tract is more effective in preventing postoperative esophageal reflux.
Collaborative Governance Model for Mangrove Ecotourism: Bintan Island Case, Indonesia
Authors: Alfiandri Alfiandri , Salomo Roy Valiant , Prasojo Eko , Wicaksono Agung
Published: December 2022

This research aims to identify gaps in indicators and variables of Collaborative Governance associated with Mangrove-based ecotourism in Bintan Island, Riau Islands, Indonesia. Furthermore, the conditions of Collaborative Governance are analyzed to obtain an ego-sectoral relationship between stakeholders with the development process carried out according to their respective visions and missions. This is qualitative research with primary and secondary data collected through interviews and transcripts. The data was analyzed using in-depth triangulation and NVivo software to determine the interpretive constructor. The results showed that stakeholders could use Mangrove conservation policies in Bintan Island to form bottom-up and top-down Collaborative Governance models. However, despite finding weak indicators and variables based on NVivo coding in the context of the priority Penta Helix model, the proposed model involving Penta Helix indicators can be implemented. Therefore, stakeholders can provide goods for Mangrove Ecotourism for domestic and foreign tourists
A systematic review and meta-analysis on the application of three-dimensional reconstruction in partial nephrectomy
Authors: Jia Li , Xingliang Yang , Jingzhen Zhu , Xiaobing Liu , Jiang Zhao , Bishao Sun , Xing Luo , Ji Zheng , Zhenqiang Fang
Published: December 2022

Objective
Three dimensional (3D) reconstruction is widely used in complicated renal surgery. This article focus on investigating the role of three-dimensional reconstruction participated in partial nephrectomy (PN) from a systematic review and meta-analysis.
Methods
We searched published studies that reported the relationship between 3D reconstruction and PN using PubMed and Embase up to December 8, 2022. STATA version 12.0was used to conduct all analyses.
Results
Seventeen studies were finally progressed to data extraction. Meta-analysis indicated a significant reduction in operation time ([SMD] =-0.34; confidence interval [CI]: -0.59 to -0.09), global ischemia time ([SMD] =-0.42; CI: -0.64 to -0.21) and opening collecting system rate (OR 0.26, 95% CI 0.14 to0.47).There are no statistically significant differences between 3d reconstruction assisted PN and 2D control group considering estimated blood loss, GFR change, positive surgical margin, length of hospital stay, and intraoperative complication.
Conclusion
Our study provides evidence that 3D reconstruction played an important role in PN, especially for complex renal tumors. However, more high-quality, larger sample size and more rigorously designed randomized controlled trials which report this topic are still needed to support our conclusions.
Intermittent superior mesenteric artery occlusion for en bloc resection of the uncinate process during laparoscopic pancreaticoduodenectomy: A novel technique
Authors: Yan Jian , Bai Zhenhua , Liu Qinqin , Zhu Taifeng , Yang Caini , Lin Shusheng , Zhang Rui , Lin Haoming , Liu Chao
Published: December 2022

Background: En bloc resection of the uncinate process of the pancreas represents the most important yet difficult step in laparoscopic pancreaticoduodenectomy (LPD), given the risk of major intraoperative bleeding which often necessitates the conversion to open surgery. We therefore developed the intermittent superior mesenteric artery occlusion (ISMAO) technique as a means of blood flow control during uncinate process resection, and investigated its role in LPD.
Methods: Consecutive patients who underwent LPD at the Department of Biliary and Pancreatic Surgery, Sun Yat-sen Memorial Hospital between August 2020 and May 2022 were enrolled. Patients were divided into 2 groups, the ISMAO and non-ISMO group. Parameters such as operation time, intraoperative blood loss volume, conversion rate to open surgery, R0 resection rate, bowel function recovery time, postoperative hospital stay length, and rate of postoperative complications (including pancreatic fistula, delayed gastric emptying, and postoperative bleeding) were compared.
Results: A total of 51 patients were included, of whom 22 (43.1%) underwent ISMAO. Significantly shorter mean operation time was observed in the ISMAOgroup compared to the non-ISMAO group (349.8 ± 84.9 min vs. 533.5 ± 105.9 min; P < 0.001). In addition, ISMAO associated with significantly lower intraoperative blood loss volume [112.5 (87.5-200.0) mL vs. 400.0 (250.0-600.0) mL; P < 0.001], and significantly lower conversion rate to open surgery (4.54% vs. 26.0%; P = 0.0485). R0 resection rate in the ISMAO group was significantly higher (95.5% vs. 79.3%; P = 0.0485). No significant differences were observed in postoperative hospital stay length, bowel function recovery time, and postoperative complication rate between the groups.
Conclusions: ISMAO represents a novel blood flow control technique for en bloc resection of the uncinate process. Our study demonstrated its role in improving surgical safety and reducing intraoperative bleeding, and suggests its potential as a standard surgical procedure in LPD.
High-Resolution Three-dimensional Azimuthal Velocity Anisotropy of S-waves in southwest Japan, based on Ambient Noise Tomography
Authors: NTHABA BOKANI , Ikeda Tatsunori , Tsuji Takeshi , Iio Yoshihisa
Published: December 2022

To investigate the dominant deformational patterns and stress conditions in the upper crustal structure of the Kinki region, southwest Japan, we constructed a high-resolution 3D azimuthal anisotropy model to a depth of ~ 11 km. We used 6-month-long ambient noise data recorded by the densely distributed permanent and temporary stations. From this dataset, cross-correlations were retrieved. We then obtained a 3D isotropic velocity model by inverting Rayleigh wave dispersion data, followed by a direct joint inversion for both 3D azimuthal anisotropy and additional isotropic velocity perturbation. The resolved 3D azimuthal anisotropy reveals significant contrasts of anisotropy across the Kinki region. The southern part of the Kinki region shows predominantly NE-SW-trending fast axes, ascribed to fossil anisotropy. The fast axes in the northwestern Kinki region are consistent with the direction of the maximum horizontal compressional stress and the principal strain rate axes, suggesting that the observed anisotropy is mainly stress-induced. On the depth profile of the anisotropy, we found depth-dependent variation of azimuthal anisotropy. There exist a significant consistency between the anisotropy observed beneath 3 km depth and the dense distribution of earthquake hypocenters (≥4 %). This interrelationship between anisotropy and seismicity demonstrates that the observed anisotropy could be linked to local crustal stress or fractures relevant to earthquake ruptures. Our high-spatial resolution 3D anisotropy model therefore contributes towards understanding the locations and features of the seismicity region.
Elemental Selenium-Derived Poly(Sulfide Selenide)s: A Platform of Photo- and Electrochemical Responsive Polymer
Authors: Yue Tian-Jun , Fu Xiang-Yu , Zhang Ji , Zhou Yong-Gui , Lu Xiao-Bing , Ren Wei-Min
Published: December 2022

Selenium-containing polymers have numerous attractive properties, such as light response behaviors, biocompatibilities, and heavy-metal-ion adhesive ability. However, the lack of efficient strategies for constructing selenium-containing polymers limits their development. Herein, a facile but efficient strategy is proposed for synthesizing poly(sulfide selenide)s directly from elemental selenium and episulfides in the presence of an organocatalyst system. This strategy affords poly(sulfide selenide)s with high sulfide–selenide linkage selectivity (> 99%). Notably, the poly(sulfide selenide)s exhibit a photodegradable nature and photo-responsive behavior via structural assembly. Furthermore, the excellent electrochemical properties of these poly(sulfide selenide)s enable their application in electrochemical CO2capture. The high monomer compatibility of this method leads to a series of poly(sulfide selenide)s with diverse structures, thus various thermal and optical performances.
Recurrent Pyloric Stenosis in a Patient with Kleefstra Syndrome: A Clinical Case Report
Authors: Bhatt Arjun , Lenfestey Mary
Published: December 2022

Background: Infantile hypertrophic pyloric stenosis refractory to initial complete pyloromyotomy is extremely rare and distinguished from incomplete pyloromyotomy by a prolonged postoperative symptomatic improvement before symptom recurrence. The pathogenesis is uncertain but is theorized to be a continuation of the process that initially produces infantile hypertrophic pyloric stenosis (IHPS), which has been associated with chromosome region 9q duplications.
Case Presentation: Here we present a case of a neonate presenting with respiratory distress syndrome, hydronephrosis, pulmonary valve stenosis, and hypotonia at birth. A microarray identified a deletion of the terminal region of chromosome 9q, consistent with Kleefstra syndrome. The infant was diagnosed with pyloric stenosis after abdominal ultrasound was ordered to investigate difficulties with feeding, vomiting, and poor weight gain. Following initial surgical repair at five weeks of age, the infant demonstrated consistent postoperative weight gain for six weeks and resolution of emesis. However, vomiting returned and weight gain halted during weeks eleven and twelve of life; a barium swallow series and repeat abdominal ultrasound demonstrated recurrent IHPS. The infant underwent repeat pyloromyotomy and with post-operative clinical improvement.
Conclusions: We present the first known case of recurrent pyloric stenosis in a patient with Kleefstra syndrome, and which further supports the hypothesis of an association between chromosome 9 and pyloric stenosis, due to an increased incidence of pyloric stenosis in chromosome 9 pathologies such as Kleefstra Syndrome found in current literature.