bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Job Crafting and Organizational Identification on Innovative Work Behavior with Work Engagement as an Intervening Variable
Authors: Novianti Winanda Dwi , Sudiarditha I Ketut R , Parimita Widya
Published: June 2023

This study aims to 1) identify and analyze the direct effect of Job Crafting on the innovative work behavior of online transportation employees; 2) know and analyze the direct effect of organizational identification on the innovative work behavior of online transportation employees; 3) know and analyzing the direct effect of work engagement on innovative work behavior of online transportation employees; 4) knowing and analyzing the direct effect of Job Crafting on online transportation employee work engagement; 5) knowing and analyzing the direct effect of organizational identification on online transportation employee work engagement; 6) knowing and analyzing the indirect effect of work engagement in mediating Job Crafting on innovative work behavior of online transportation employees; 7) knowing and analyzing the indirect effect of work engagement in mediating organizational identification on innovative work behavior of online transportation employees. The research was conducted on 360 online transportation employees. The data analysis technique uses the Structural Equation Model.
The results showed that 1) there is a direct effect of Job Crafting on the innovative work behavior of online transportation employees; 2) there is a direct effect of organizational identification on the innovative work behavior of online transportation employees; 3) there is a direct effect of work engagement on innovative work behavior of online transportation employees; 4) there is a direct effect of Job Crafting on online transportation employee work engagement; 5) there is a direct effect of organizational identification on online transportation employee work engagement; 6) there is an indirect effect of work engagement in mediating Job Crafting on innovative work behavior of online transportation employees; 7) there is an indirect effect of work engagement in mediating organizational identification on innovative work behavior of online transportation employees.
The Effect of Work Environment and Work Discipline on Employee Performance at the South Cikarang Primary Tax Service Office Through Work Motivation
Authors: Mulana Rudi , Silitonga Eddy Sanusi , Hoesin Wagiarto
Published: June 2023

This study aims to find out 1). Effect of work environment and work discipline on work motivation, 2) Effect of work environment and work discipline on employee performance, 3) Effect of work motivation on employee performance, 4) Effect of work environment and work discipline on employee performance through work motivation. The research was conducted at the South Cikarang Primary Tax Service Office (KPP). The number of respondents was 57 people with event sampling techniques and data analysis using descriptive analysis and path analysis. Research results show that 1). There is an influence of work environment and work discipline on work motivation, 2) There is an influence of work environment and work discipline on employee performance, 3) There is an influence of work motivation on employee performance, 4) Motivation cannot influence work environment and work discipline on employee performance.
Data that Matter: On Metaphors of Obfuscation, Thinking ‘the Digital’ as Material and Posthuman Cooperation with AI
Authors: Ring Annie
Published: June 2023

This article argues that ‘the digital’ and ‘big data’ are metaphors of obfuscation, which are used to screen the real effects of technologies on lived experiences and the planet. Now that technology consumers are connected 24/7 to the Internet (or ‘Web’), their data can be gathered and monetized on a vast scale. The new data economies and AI technologies that have emerged as a result require careful evaluation regarding their effects on bodies, environments and new forms of knowledge. In this piece, I therefore lay out the material impacts of so-called digital phenomena: of data, their large-scale storage in the ‘Cloud’, and their use in training algorithms and emergent forms of artificial intelligence (AI). Building on scholarship by cultural theorists of technology including Donna Haraway, N. Katherine Hayles, Wendy Hui Kyong Chun and Elena Esposito, as well as long-standing philosophies of metaphor and violence by Friedrich Nietzsche, Karl Marx and Hannah Arendt, I make the case that thinking about new media and technology is more ethical where it is less metaphorical, and so more conscious of the entangled nature of technology with human and posthuman life, including AI. The resulting concept of data that matter is proposed with a view to more justice-oriented uses of data and machine cognition in the future.
Unbecoming Woman: The Shadow Feminism of King Kong théorie by Virginie Despentes
Authors: Pugh Alexandra
Published: June 2023

This article establishes a dialogue between Virginie Despentes’s 2006 memoir-cum-manifesto, King Kong théorie and Jack Halberstam’s theorization of ‘shadow feminism’. For Halberstam, ‘not succeeding at womanhood can offer unexpected pleasures (…) Shadow feminisms take the form not of becoming, being, and doing but of shady, murky modes of undoing, un-becoming, and violating’. In King Kong théorie, I argue, Despentes embraces her failure to ‘become woman’, and her accounts of rape and rape fantasy present a refusal of mastery wherein the subject might unravel, come undone. Through her use of the King Kong metaphor, Despentes connects ‘unbecoming woman’ to an unravelling of the human subject; King Kong figures in the text as a composite, cyborgian creature, with whom Despentes herself identifies. In King Kong théorie, then, Despentes adopts a shadowy, hybrid positionality, forging a textual space for all creatures who fail to be or become woman.
Pengesahan Nikah pada Pasangan di Bawah Umur
Authors: Muhammad Nafi
Published: June 2023

This article discusses the legal considerations of judges in adjudicating marriage certificate cases filed when the parties are minors. The legal problem is that the endorsement of an underage marriage is considered a violation of the law. This research is empirical or sociological legal research of a descriptive nature. Primary and secondary data types that include primary, secondary, and tertiary legal materials. The data collection techniques used are field studies and literature studies, then technical analysis used is qualitative methods with interactive analysis models. Data collection is carried out by documentation techniques, and literature studies. The results of this study showed that the Explanation of the Clerk of the Supreme Court Number 231/PAN/Hk.05/1/2019, answered the letter from the Director General of The Directorate General of Religious Affairs Number 472.2/18752/DUKCAPIL, which explained that the application for marriage certificate submitted by an underage couple must be rejected by the Religious Court, because it is considered a form of violation of the law.  But in its implementation the judge looked at the conditions of the maslahat that were dug out of the examination at the trial.
PainVision-based evaluation of brain potentials: a novel approach for quantitative pain assessment
Authors: Chen Li , Zhang Zhen , Han Rui , Du Liyuan , Li Zhenxing , Liu Shuiping , Huang Dong , Zhou Haocheng
Published: June 2023

Introduction: The complex and multidimensional nature of pain poses a major challenge in clinical pain assessments. In this study, we aimed to evaluate a novel approach combining quantitative sensory testing (QST) with event-related potential measurements for assessment of experimental pain in healthy individuals.

Methods: QST was performed with a commercial device (PainVision, PS-2100), and numeric rating scale (NRS) scores after exposure to different sensory stimuli were reported by the participants. Resting-state electroencephalography (EEG) was simultaneously performed to capture the cortical responses to peripheral stimulation.

Results: Pain scores increased with the intensity of stimuli, with mean NRS scores of 2.7 ± 1.0 after mild stimuli and 5.6 ± 1.0 after moderate stimuli. A reproducible, significant P2-N2 complex was evoked by both mild and moderately painful stimuli, but not by non-painful stimuli. The latency of pain-related potentials was not significantly different between stimuli. The amplitudes of both P2 and N2 components significantly increased when intense nociception was applied, and the increments mainly originated from theta oscillations.

Conclusion: The combination of QST with EEG was feasible for subjective and objective pain assessment. Distinct patterns of brain potentials were associated with the phenotype of the peripheral stimuli (e.g., noxious versus. innoxious, high versus. low pain intensity).
Elevated levels of enteric IgA in an unimmunised mouse model of Hyper IgM syndrome derived from gut-associated secondary lymph organs even in the absence of germinal centres
Authors: Hernandez-Cazares Felipe , Maqueda-Alfaro Raul Antonio , Lopez-Saucedo Catalina , Martinez-Barnetche Jesus , Yam-Puc Juan Carlos , Estrada-Parra Sergio , Flores-Romo Leopoldo , Estrada-Garcia Teresa
Published: June 2023

Introduction

Patients with Human Hyper IgM syndromes (HIGM) developed pulmonary and gastrointestinal infections since infancy and most patients have mutations in the CD40 ligand (CD40L) gene. Most HIGM patients compared to healthy subjects have higher/similar IgM and lower IgG, and IgA serum concentrations but gut antibody concentrations are unknown. CD40L on activated T-cells interacts with CD40 on B-cells, essential for the formation of germinal centres (GCs) inside secondary lymphoid organs (SLOs), where high-affinity antibodies, long-lived antibody-secreting plasma cells, and memory B-cells, are produced. C57BL6-CD40 ligand deficient mice (C57BL6-cd40l−/−), are a model of HIGM, because serum immunoglobulin concentrations parallel levels observed in HIGM patients and have higher faecal IgA concentrations. In mice, TGFβ and other cytokines induce IgA production.

Aims

To compare and evaluate B-cell populations and IgA-producing plasma cells in peritoneal lavage, non-gut-associated SLOs, spleen/inguinal lymph nodes (ILN), and gut-associated SLOs, mesenteric lymph nodes (MLN)/Peyer´s patches (PP) of unimmunised C57BL6-cd40l−/− and C57BL6-wild-type (WT) mice.

Material and methods

Peritoneal lavages, spleens, ILN, MLN, and PP from 8-10 weeks old C57BL6-cd40l−/− and WT mice, were obtained. Organ cryosections were analysed by immunofluorescence and B-cell populations and IgA-positive plasma cell suspensions by flow cytometry.

Results

In unimmunised WT mice, GCs were only observed in the gut-associated SLOs, but GCs were absent in all C57BL6-cd40l−/− SLOs. PP and MLN of C57BL6-cd40l−/− mice exhibited a significantly higher number of IgA-producing cells than WT mice. In the spleen and ILN of C57BL6-cd40l−/− mice IgA-producing cells significantly decreased, while IgM-positive plasma cells increased. C57BL6-cd40l−/− B-1 cells were more abundant in all analysed SLOs, whereas in WT mice most B-1 cells were contained within the peritoneal cavity. C57BL6-cd40l−/− B-cells in MLN expressed a higher TGFβ receptor-1 than WT mice. Mouse strains small intestine microvilli (MV), have a similar frequency of IgA-positive cells.

Discussion

Together our results confirm the role of PP and MLN as gut inductive sites, whose characteristic features are to initiate an IgA preferential immune response production in these anatomical sites even in the absence of GCs. IgA antibodies play a pivotal role in neutralising, eliminating, and regulating potential pathogens and microorganisms in the gut.
Abnormal plasma ceramides refine high-risk patients with worsening heart failure
Authors: Ren Lu , Li Fengjuan , Tan Xin , Fan Yangkai , Ke Bingbing , Zhang Yixin , Jiang Hongfeng , Jia Lixin , Wang Yuan , Du Jie
Published: June 2023

Background

Worsening heart failure (WHF) is a heterogeneous clinical syndrome with poor prognosis. More effective risk stratification tools are required to identify high-risk patients. Evidence suggest that aberrant ceramide accumulation can be affected by heart failure risk factors and as a driver of tissue damage. We hypothesized that specific ceramide lengths and ratios serve as biomarkers for risk stratification in WHF patients by reflecting pathological changes of distinct organ dysfunctions.

Medthods

We measured seven plasma ceramides using liquid chromatography-mass spectrometry (LC-MS) in 1,558 patients, including 1,262 participants in retrospective discovery set and 296 WHF patients in prospective validation set in BIOMS-HF study (Registry Study of Biomarkers in Heart Failure). Univariable and multivariable logistic regression models were constructed to identify associations of ceramides with organ dysfunctions.

Results

We constructed three ceramide-based scores linked independently to heart, liver, and kidney dysfunction, with ceramides and ratios included in each score specifying systemic inflammation, chronic metabolic disorder, and water-sodium retention. The combined ceramide heart failure score (CHFS) was independently associated with adverse outcomes [Hazard Ratio, 2.80 (95% CI: 1.78–4.40; P < 0.001); 2.68 995% CI: 1.12–6.46; P = 0.028)] and improved the predictive value of Acute Decompensated Heart Failure National Registry score and BNP [net reclassification index, 0.34 (95% confidence interval, CI: 0.19–0.50); 0.42 (95% CI: 0.13–0.70)] in the discovery and validation set, respectively. Lower BNP levels, but higher CHFS had the highest hazard of future adverse events in WHF patients.

Conclusion

Abnormal plasma ceramides, associated with heart and peripheral organ dysfunctions, provide incremental prognostic information over the ADHERE score and brain natriuretic peptide concentration for risk stratification in WHF patients. This may facilitate the reclassification of high-risk patients in need of aggressive therapeutic interventions.
Toward asset-based LatCrit pedagogies in STEM: centering Latine students’ strengths to reimagine STEM teaching and practice
Authors: Hernandez Negrete Ambar , Mouavangsou Kaozong N. , Caporale Natalia
Published: June 2023

Latine students continue to persist in science, technology, engineering and mathematics (STEM) fields despite the numerous obstacles in place that stifle their academic potential and contributions. Instead of fostering the strengths Latine students possess that help them succeed despite these obstacles, the field of STEM education has traditionally examined these students’ experiences and challenges through a deficit lens. Deficit perspectives posit that any existing disparities in educational outcomes in STEM for Latine students are a product of the students’ lack of interest in STEM fields, poor academic preparation and/or motivation, among other ‘faults.’ In this manner, this deficit approach absolves educators, educational institutions, administrators, and researchers from any responsibility in mediating the disparate outcomes and negates the roles that outdated pedagogical practices, structural racism, discrimination and disciplinary bias have in limiting Latine students’ success in STEM. These deficit-understandings of these inequities are pervasive in all aspects of STEM education, guiding curricular choices, pedagogical approaches, assessment designs, interventions and even how STEM fields define knowledge and success. To counter these harmful constructions, this article discusses how STEM educators can draw on Latino Critical Race Theory (LatCrit) and Community Cultural Wealth (CCW) epistemologies to foster learning ecologies that draw on Latine students’ cultural strengths rather than deficits. To this end, this article introduces LatCrit and CCW frameworks in the context of STEM education, and combines them to propose an asset-based LatCrit pedagogical approach to STEM curriculum design and teaching. It also contributes guiding questions and application examples STEM educators can reference to advance asset-based LatCrit pedagogical approaches that promote justice and equity within STEM classrooms and beyond. Contributing to this underdeveloped line of scholarship in the field of STEM, we apply these critical frames to help educators (re)imagine postsecondary STEM pedagogies and reforms around the wealth of skills, dispositions, and cultural practices that Latine students possess.
Elevated Chinese visceral adiposity index increases the risk of stroke in Chinese patients with metabolic syndrome
Authors: Liu Zeyu , Huang Qin , Deng Bi , Wei Minping , Feng Xianjing , Yu Fang , Feng Jie , Du Yang , Xia Jian
Published: June 2023

Introduction

Patients with Metabolic Syndrome (MetS) are considered at high-risk for incident stroke. An indicator of visceral adiposity dysfunction, the Chinese Visceral Adiposity Index (CVAI) is used to evaluate the dysfunction of visceral fat. Given the impact of visceral adiposity dysfunction on elevating cardiovascular hazards, this study aimed to examine the association between CVAI and stroke risk in MetS patients.

Method

Between November 2017 and December 2018, a total of 18,974 individuals aged ≥40 underwent standardized in-person clinical interviews in Hunan Province, with 6,732 meeting the criteria for MetS. After the baseline survey was completed, subsequent surveys were conducted biennially. The study was split into two stages performed at baseline and after two years. During the former, receiver-operating characteristic curves were used to assess the accuracy of using baseline CVAI in diagnosing MetS. After two years, we examined the association between CVAI and incident stroke in MetS patients using logistic regression, subgroup analysis, and restricted cubic spline (RCS) analysis.

Result

As evidenced by a higher AUC (AUC:0.741), CVAI demonstrated superior diagnostic performance relative to body mass index (AUC:0.631) and waist circumference (AUC:0.627) in diagnosing MetS. After a 2-year follow-up, 72 MetS patients had a stroke event. There was a robust positive correlation between incident stroke and CVAI in patients with MetS. Each 1 SD increase in CVAI was associated with a 1.52-fold higher risk of stroke after adjustment for confounding factors (aOR=1.52, 95%CI: 1.18-1.95). The RCS demonstrated a reduced risk of stroke for MetS patients when the CVAI was below 110.91. However, no significant correlation was detected between CVAI and stroke in non-MetS patients.

Conclusion

Our findings recommend CVAI as a superior screening tool for detecting MetS and suggest that reducing CVAI can mitigate the risk of stroke in patients with MetS.
CD26lowPD-1+ CD8 T cells are terminally exhausted and associated with leukemia progression in acute myeloid leukemia
Authors: Zhou Huarong , Jia Bei , Annageldiyev Charyguly , Minagawa Kentaro , Zhao Chenchen , Mineishi Shin , Ehmann W Christopher , Naik Seema G. , Cioccio Joseph , Wirk Baldeep , Songdej Natthapol , Rakszawski Kevin L. , Nickolich Myles S. , Shen Jianzhen , Zheng Hong
Published: June 2023

Acute myeloid leukemia (AML) is a devastating blood cancer with poor prognosis. Novel effective treatment is an urgent unmet need. Immunotherapy targeting T cell exhaustion by blocking inhibitory pathways, such as PD-1, is promising in cancer treatment. However, results from clinical studies applying PD-1 blockade to AML patients are largely disappointing. AML is highly heterogeneous. Identification of additional immune regulatory pathways and defining predictive biomarkers for treatment response are crucial to optimize the strategy. CD26 is a marker of T cell activation and involved in multiple immune processes. Here, we performed comprehensive phenotypic and functional analyses on the blood samples collected from AML patients and discovered that CD26lowPD-1+ CD8 T cells were associated with AML progression. Specifically, the percentage of this cell fraction was significantly higher in patients with newly diagnosed AML compared to that in patients achieved completed remission or healthy controls. Our subsequent studies on CD26lowPD-1+ CD8 T cells from AML patients at initial diagnosis demonstrated that this cell population highly expressed inhibitory receptors and displayed impaired cytokine production, indicating an exhaustion status. Importantly, CD26lowPD-1+ CD8 T cells carried features of terminal exhaustion, manifested by higher frequency of TEMRA differentiation, increased expression of transcription factors that are observed in terminally exhausted T cells, and high level of intracellular expression of granzyme B and perforin. Our findings suggest a prognostic and predictive value of CD26 in AML, providing pivotal information to optimize the immunotherapy for this devastating cancer.
Extracellular vesicles derived from Talaromyces marneffei contain immunogenic compounds and modulate THP-1 macrophage responses
Authors: Pruksaphon Kritsada , Amsri Artid , Thammasit Patcharin , Nosanchuk Joshua D. , Youngchim Sirida
Published: June 2023

Pathogenic eukaryotes including fungi release extracellular vesicles (EVs) which are composed of a variety of bioactive components, including peptides, nucleic acids, polysaccharides, and membrane lipids. EVs contain virulence-associated molecules suggesting a crucial role of these structures in disease pathogenesis. EVs derived from the pathogenic yeast phase of Talaromyces (Penicillium) marneffei, a causative agent of systemic opportunistic mycoses “talaromycosis,” were studied for their immunogenic components and immunomodulatory properties. Some important virulence factors in EVs including fungal melanin and yeast phase specific mannoprotein were determined by immunoblotting. Furthermore, fluorescence microscopy revealed that T. marneffei EVs were internalized by THP-1 human macrophages. Co-incubation of T. marneffei EVs with THP-1 human macrophages resulted in increased levels of supernatant interleukin (IL)-1β, IL-6 and IL-10. The expression of THP-1 macrophage surface CD86 was significantly increased after exposed to T. marneffei EVs. These findings support the hypothesis that fungal EVs play an important role in macrophage “classical” M1 polarization. T. marneffei EVs preparations also increased phagocytosis, suggesting that EV components stimulate THP-1 macrophages to produce effective antimicrobial compounds. In addition, T. marneffei EVs stimulated THP-1 macrophages were more effective at killing T. marneffei conidia. These results indicate that T. marneffei EVs can potently modulate macrophage functions, resulting in the activation of these innate immune cells to enhance their antimicrobial activity.
Mitochondrial DNA methylation is a predictor of immunotherapy response and prognosis in breast cancer: scRNA-seq and bulk-seq data insights
Authors: Ma Yixuan , Du Juan , Chen Meini , Gao Ning , Wang Sijia , Mi Zhikuan , Wei Xiaoli , Zhao Jumei
Published: June 2023

Background

Alterations in Mitochondrial DNA methylation (MTDM) exist in many tumors, but their role in breast cancer (BC) development remains unclear.

Methods

We analyzed BC patient data by combining scRNA-seq and bulk sequencing. Weighted co-expression network analysis (WGCNA) of TCGA data identified mitochondrial DNA methylation (MTDM)-associated genes in BC. COX regression and LASSO regression were used to build prognostic models. The biological function of MTDM was assessed using various methods, such as signaling pathway enrichment analysis, copynumber karyotyping analysis, and quantitative analysis of the cell proliferation rate. We also evaluated MTDM-mediated alterations in the immune microenvironment using immune microenvironment, microsatellite instability, mutation, unsupervised clustering, malignant cell subtype differentiation, immune cell subtype differentiation, and cell-communication signature analyses. Finally, we performed cellular experiments to validate the role of the MTDM-associated prognostic gene NCAPD3 in BC.

Results

In this study, MTDM-associated prognostic models divided BC patients into high/low MTDM groups in TCGA/GEO datasets. The difference in survival time between the two groups was statistically significant (P<0.001). We found that high MTDM status was positively correlated with tumor cell proliferation. We analyzed the immune microenvironment and found that low-MTDM group had higher immune checkpoint gene expression/immune cell infiltration, which could lead to potential benefits from immunotherapy. In contrast, the high MTDM group had higher proliferation rates and levels of CD8+T cell exhaustion, which may be related to the secretion of GDF15 by malignant breast epithelial cells with a high MTDM status. Cellular experiments validated the role of the MTDM-associated prognostic gene NCAPD3 (the gene most positively correlated with epithelial malignant cell proliferation in the model) in BC. Knockdown of NCAPD3 significantly reduced the activity and proliferation of MDA-MB-231 and BCAP-37 cells, and significantly reduced their migration ability of BCAP-37 cell line.

Conclusion

This study presented a holistic evaluation of the multifaceted roles of MTDM in BC. The analysis of MTDM levels not only enables the prediction of response to immunotherapy but also serves as an accurate prognostic indicator for patients with BC. These insightful discoveries provide novel perspectives on tumor immunity and have the potentially to revolutionize the diagnosis and treatment of BC.
Analysis of an improved Nishihara creep model considering plastic strain accumulation
Authors: Cheng Yang , Zhang Liangliang
Published: June 2023

Rock-engineering structures, particularly subway excavation rock-engineering structures, are subjected to the long-term effects of external loading that may gradually damage and cause creep and severe plastic deformations or even progressive failure. The traditional Nishihara creep model cannot accurately describe the accelerated creep stage of rock with particularly obvious nonlinear characteristics. Based on this model, a damage variable that can consider the plastic strain accumulation is introduced in this study to replace the viscoplastic body in the traditional model with a damaged viscoplastic body and establish an improved Nishihara creep model. Using the superposition principle, the creep equations of the improved model in the one- and three-dimensional stress states are derived. The accuracy and rationality of the improved model are verified using uniaxial and conventional triaxial creep tests of mudstone and sandstone under different confining pressures. The improved model can not only accurately reflect the nonlinear characteristics of the creep curve in the decay and constant velocity stages but also describe the accelerated creep characteristics of mudstone and sandstone in a high-stress state. Its applicability and accuracy are superior to those of the traditional model. This study can provide a theoretical reference for subway tunnel excavation and stability analysis of deep roadway surrounding rocks.
Improvement in health-related quality of life with Botulinum toxin A injection in acquired superior oblique palsy
Authors: Chen Chonglin , Xu Meiping , Yu Huanyun , Li Yipao , Yu Xinping
Published: June 2023

Purpose

This study aimed to investigate the outcomes of Botulinum toxin A (BTA) injection into the inferior oblique (IO) muscle for the management of unilateral acute acquired superior oblique palsy (SOP) and to evaluate changes in health-related quality of life post-injection using the Adult Strabismus-20 (AS-20) questionnaire.

Methods

A prospective cohort study was performed in patients with unilateral acute acquired SOP who received BTA injections. Four units of BTA were injected into the ipsilateral IO muscle. Ocular examinations were performed pre-and post-injection, including alignment, ocular movement, and cyclotorsion deviation. The patients’ AS-20 questionnaire scores were analyzed.

Results

A total of 21 patients with acute acquired SOP were included. The initial median vertical deviation was 5 PD (range 1–16), which was improved to 0 PD (range 0–10) at both 1 and 6 months post-injection (p < 0.001 and p < 0.001, respectively). The median torsional deviation was 7° (range 2–18) at baseline and resolved to 0 degrees (range −3–5) at the 1-month and 0° (range −2–7) at the 6-month follow-up (p < 0.001 and p < 0.001, respectively). There were significant increases in the overall score (OAS), psychosocial subscale score (PSS), and functional subscale score (FSS) from baseline values at both the 1-month (p < 0.001, p < 0.001, and p = 0.001, respectively) and 6-month follow-up (all p < 0.001).

Conclusion

Injecting BTA into the ipsilateral IO muscle successfully resolved vertical and torsional deviations and significantly improved quality-of-life scores. Our findings show that BTA treatment, as an early treatment for acute acquired SOP, can help patients by significantly improving their quality of life.
Effect of mindfulness-based mind-body therapies in patients with non-specific low back pain—A network meta-analysis of randomized controlled trials
Authors: Yang Huanying , Wang Xiangfu , Wang Xuetao , Yang Jianxia , Zhang Wanqian , Ding Yanfang , Sang Tingrui , Chen Weiguo , Wang Wanhong
Published: June 2023

Background/objectives

Although mindfulness-based mind-body therapy (MBMBT) is an effective non-surgical treatment for patients with non-specific low back pain (NLBP), the best MBMBT mode of treatment for NLBP patients has not been identified. Therefore, a network meta-analysis (NMA) was conducted to compare the effects of different MBMBTs in the treatment of NLBP patients.

Methods

PubMed, EMBASE, Cochrane Central Register of Controlled Trials, and Web of Science databases were searched for randomized controlled trials (RCTs) applying MBMBT for the treatment of NLBP patients, with all of the searches ranging from the time of database creation to January 2023. After 2 researchers independently screened the literature, extracted information, and evaluated the risks of biases in the included studies, the data were analyzed by using Stata 16.0 software.

Results

A total of 46 RCTs were included, including 3,886 NLBP patients and 9 MBMBT (Yoga, Ayurvedic Massage, Pilates, Craniosacral Therapy, Meditation, Meditation + Yoga, Qigong, Tai Chi, and Dance). The results of the NMA showed that Craniosacral Therapy [surface under the cumulative ranking (SUCRA): 99.2 and 99.5%] ranked the highest in terms of improving pain and disability, followed by Other Manipulations (SUCRA: 80.6 and 90.8%) and Pilates (SUCRA: 54.5 and 71.2%). In terms of improving physical health, Craniosacral Therapy (SUCRA: 100%) ranked the highest, followed by Pilates (SUCRA: 72.3%) and Meditation (SUCRA: 55.9%). In terms of improving mental health, Craniosacral Therapy (SUCRA: 100%) ranked the highest, followed by Meditation (SUCRA: 70.7%) and Pilates (SUCRA: 63.2%). However, in terms of improving pain, physical health, and mental health, Usual Care (SUCRA: 7.0, 14.2, and 11.8%, respectively) ranked lowest. Moreover, in terms of improving disability, Dance (SUCRA: 11.3%) ranked lowest.

Conclusion

This NMA shows that Craniosacral Therapy may be the most effective MBMBT in treating NLBP patients and deserves to be promoted for clinical use.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/, PROSPERO [CRD42023389369].
Notch signaling as a master regulator of adult neurogenesis
Authors: Lampada Aikaterini , Taylor Verdon
Published: June 2023

Neurogenesis ceases in most regions of the mammalian brain before or shortly after birth, however, in a few restricted brain regions, the production of new neurons proceeds into adulthood. Neural stem cells (NSCs) in these neurogenic zones are integrated into niches that control their activity and fate. Most stem cells in the adult brain are mitotically inactive and these cells can remain quiescent for months or even years. One of the key questions is what are the molecular mechanisms that regulate NSC maintenance and differentiation. Notch signaling has been shown to be a critical regulator of stem cell activity and maintenance in many tissues including in the nervous system. In this mini-review we discuss the roles of Notch signaling and the functions of the different Notch receptors and ligands in regulating neurogenesis in the adult murine brain. We review the functions of Notch signaling components in controlling NSC quiescence and entry into cell cycle and neurogenesis.
Meningiomas with CNS invasion
Authors: Gousias Konstantinos , Trakolis Leonidas , Simon Matthias
Published: June 2023

CNS invasion has been included as an independent criterion for the diagnosis of a high-grade (WHO and CNS grade 2 and 3) meningioma in the 2016 and more recently in the 2021 WHO classification. However, the prognostic role of brain invasion has recently been questioned. Also, surgical treatment for brain invasive meningiomas may pose specific challenges. We conducted a systematic review of the 2016–2022 literature on brain invasive meningiomas in Pubmed, Scopus, Web of Science and the Cochrane Library. The prognostic relevance of brain invasion as a stand-alone criterion is still unclear. Additional and larger studies using robust definitions of histological brain invasion and addressing the issue of sampling errors are clearly warranted. Although the necessity of molecular profiling in meningioma grading, prognostication and decision making in the future is obvious, specific markers for brain invasion are lacking for the time being. Advanced neuroimaging may predict CNS invasion preoperatively. The extent of resection (e.g., the Simpson grading) is an important predictor of tumor recurrence especially in higher grade meningiomas, but also – although likely to a lesser degree – in benign tumors, and therefore also in brain invasive meningiomas with and without other histological features of atypia or malignancy. Hence, surgery for brain invasive meningiomas should follow the principles of maximal but safe resections. There are some data to suggest that safety and functional outcomes in such cases may benefit from the armamentarium of surgical adjuncts commonly used for surgery of eloquent gliomas such as intraoperative monitoring, awake craniotomy, DTI tractography and further advanced intraoperative brain tumor visualization.
Potential role of creatine as an anticonvulsant agent: evidence from preclinical studies
Authors: Alraddadi Eman A. , Khojah Abdulrahman M. , Alamri Faisal F. , Kecheck Husun K. , Altaf Wid F. , Khouqeer Yousef
Published: June 2023

Epilepsy is one of the most common neurological disorders affecting people of all ages representing a significant social and public health burden. Current therapeutic options for epilepsy are not effective in a significant proportion of patients suggesting a need for identifying novel targets for the development of more effective therapeutics. There is growing evidence from animal and human studies suggesting a role of impaired brain energy metabolism and mitochondrial dysfunction in the development of epilepsy. Candidate compounds with the potential to target brain energetics have promising future in the management of epilepsy and other related neurological disorders. Creatine is a naturally occurring organic compound that serves as an energy buffer and energy shuttle in tissues, such as brain and skeletal muscle, that exhibit dynamic energy requirements. In this review, applications of creatine supplements in neurological conditions in which mitochondrial dysfunction is a central component in its pathology will be discussed. Currently, limited evidence mainly from preclinical animal studies suggest anticonvulsant properties of creatine; however, the exact mechanism remain to be elucidated. Future work should involve larger clinical trials of creatine used as an add-on therapy, followed by large clinical trials of creatine as monotherapy.
Case report: SLC6A1 mutations presenting with isolated absence seizures: description of 2 novel cases
Authors: Caputo Davide , Franceschetti Silvana , Castellotti Barbara , Freri Elena , Zorzi G. , Saletti Veronica , Canafoglia Laura , Granata Tiziana
Published: June 2023

We report the clinical and EEG data of two patients harboring heterozygous SLC6A1 mutations, who presented with typical absence seizures at 3 Hz spike and wave as well as with mild cognitive disability. Neuroradiological and other laboratory investigations were normal. Our observations suggest that SLC6A1 mutations can be suspected in children with typical absences as the only seizure type, especially if associated with, even mild, cognitive deficits.
A non-mutated TROP2 fingerprint in cancer genetics
Authors: Guerra Emanuela , Di Pietro Roberta , Stati Gianmarco , Alberti Saverio
Published: June 2023

The advent of high throughput DNA sequencing is providing massive amounts of tumor-associated mutation data. Implicit in these analyses is the assumption that, by acquiring a series of hallmark changes, normal cells evolve along a neoplastic path. However, the lack of correlation between cancer risk and global exposure to mutagenic factors provides arguments against this model. This suggested that additional, non-mutagenic factors are at work in cancer development. A candidate determinant is TROP2, that stands out for its expression in the majority of solid tumors in human, for its impact on the prognosis of most solid cancers and for its role as driver of cancer growth and metastatic diffusion, through overexpression as a wild-type form. The Trop-2 signaling network encompasses CREB1, Jun, NF-κB, Rb, STAT1 and STAT3, through induction of cyclin D1 and MAPK/ERK. Notably, Trop-2-driven pathways vastly overlap with those activated by most functionally relevant/most frequently mutated RAS and TP53, and are co-expressed in a large fraction of individual tumor cases, suggesting functional overlap. Mutated Ras was shown to synergize with the TROP2-CYCLIND1 mRNA chimera in transforming primary cells into tumorigenic ones. Genomic loss of TROP2 was found to promote carcinogenesis in squamous cell carcinomas through modulation of Src and mutated Ras pathways. DNA methylation and TP53 status were shown to cause genome instability and TROP gene amplification, together with Trop-2 protein overexpression. These findings suggest that mutagenic and the TROP2 non-mutagenic pathways deeply intertwine in driving transformed cell growth and malignant progression of solid cancers.
Clinical features and management of atypical hemolytic uremic syndrome patient with DGKE gene variants: a case report
Authors: Dai Xiaomei , Ma Yu , Lin Qiang , Tang Hanyun , Chen Ruyue , Zhu Yun , Shen Yunyan , Cui Ningxun , Hong Zhongqin , Li Yanhong , Li Xiaozhong
Published: June 2023

Background

Atypical hemolytic uremic syndrome (aHUS) with diacylglycerol kinase epsilon (DGKE) gene variant is a rare variant of thrombotic microangiopathy (TMA). The information on the clinical features, management and long-term outcomes of DGKE-aHUS patients have not yet been fully elucidated. The aim of this study was to report a novel variant of the DGKE gene in a Chinese population with aHUS.

Case presentation

The present work reports a 7-month-old boy with aHUS, possibly triggered by gastrointestinal infection, without complement activation, with little response to plasma therapy and nephroprotective measures. The patient died during the 8th week of his hospital stay. The causes of death were intracranial hemorrhage and multiorgan dysfunction. Comprehensive WES of peripheral blood-derived DNA revealed two heterozygous variations in the DGKE exon region: NM_003647.2, c.610dup, p.Thr204Asnfs*4 and deletion of exons 4–6.

Conclusions

This case suggest that atypical HUS with DGKE gene variant has a poor prognosis with a high mortality rate, which typically manifests in the first year of life and presents as a systemic disease with early-onset HUS with rapidly worsening renal function and chronic proteinuria. There is no specific treatment for DGKE-aHUS. There have an uncertain benefit of plasma therapy for DGKE-aHUS patients. The literature demonstrated that anti-complement therapy showed benefits for DGKE-aHUS with complement activation and autoantibodies during the overt TMA presentation but did not prevent TMA relapses. Early diagnosis and treatment may prevent complications and improve prognosis.
No signs of check-list fatigue – introducing the StOP? intra-operative briefing enhances the quality of an established pre-operative briefing in a pre-post intervention study
Authors: Timm-Holzer Eliane , Tschan Franziska , Keller Sandra , Semmer Norbert K. , Zimmermann Jasmin , Huber Simon A. , Hübner Martin , Candinas Daniel , Demartines Nicolas , Weber Markus , Beldi Guido
Published: June 2023

Background

The team timeout (TTO) is a safety checklist to be performed by the surgical team prior to incision. Exchange of critical information is, however, important not only before but also during an operation and members of surgical teams frequently feel insufficiently informed by the operating surgeon about the ongoing procedure. To improve the exchange of critical information during surgery, the StOP?-protocol was developed: At appropriate moments during the procedure, the leading surgeon briefly interrupts the operation and informs the team about the current Status (St) and next steps/objectives (O) of the operation, as well as possible Problems (P), and encourages questions of other team members (?). The StOP?-protocol draws attention to the team. Anticipating the occurrence of StOP?-protocols may support awareness of team processes and quality issues from the beginning and thus support other interventions such as the TTO; however, it also may signal an additional demand and contribute to a phenomenon akin to “checklist fatigue.” We investigated if, and how, the introduction of the StOP?-protocol influenced TTO quality.

Methods

This was a prospective intervention study employing a pre-post design. In the visceral surgical departments of two university hospitals and one urban hospital the quality of 356 timeouts (out of 371 included operation) was assessed by external observers before (154) and after (202) the introduction of the StOP?-briefing. Timeout quality was rated in terms of timeout completeness (number of checklist items mentioned) and timeout quality (engagement, pace, social atmosphere, noise).

Results

As compared to the baseline, after the implementation of the StOP?-protocol, observed timeouts had higher completeness ratings (F = 8.69, p = 0.003) and were rated by observers as higher in engagement (F = 13.48, p < 0.001), less rushed (F = 14.85, p < 0.001), in a better social atmosphere (F = 5.83, p < 0.016) and less noisy (F = 5.35, p < 0.022).

Conclusion

Aspects of TTO are affected by the anticipation of StOP?-protocols. However, rather than harming the timeout goals by inducing “checklist fatigue,” it increases completeness and quality of the team timeout.
Psychedelic Assisted Psychotherapy preparing your target using psychohistoriography: a Jamaican perspective
Authors: De La Haye Winston , Walcott Geoffrey , Eaton Jordan , Beckford Jhoelle , Greene Janelle
Published: June 2023

The efficacy of psilocybin and other psychedelics as modes of treatment have been demonstrated through clinical trials and other studies in the management of a number of mental illnesses, including some treatment resistant cases. In Psychedelic Assisted Psychotherapy (PAP), psychedelics catalyze or enhance the experience fostered by psychotherapeutic methods. Psychohistoriographic Brief Psychotherapy, conceptualized by the late Professor Frederick Hickling in the 1970′s in Kingston, Jamaica, offers a pathway for exploration in the Jamaican context. Applied to individuals, Psychohistoriographic Brief Therapy (PBT) has already shown success in patients with personality disorders in Jamaica through a process which includes documenting life experiences in a psychohistoriogram. In the De La Haye psilocybin Treatment Protocol (DPTP), micro-doses of crushed, dried psilocybin mushrooms are taken throughout an 8-week outpatient process of documenting the components of the psychohistoriogram, making use of the increased openness and empathy associated with the use of psychedelic agents. These sessions are followed by supervised in-office therapeutic/mystical doses of crushed, dried psilocybin mushrooms in the 9th week. Given the legal status and availability of psilocybin containing products in a few countries like Jamaica, there is a potential role for a regulated psychedelic industry contributing to the body of useful and rigorous clinical research which is needed in this area. Clients could benefit as we venture into this new frontier in psychiatry.
A cost-effectiveness analysis of the 13-valent pneumococcal conjugated vaccine and the 23-valent pneumococcal polysaccharide vaccine among Thai older adult
Authors: Ngamprasertchai Thundon , Kositamongkol Chayanis , Lawpoolsri Saranath , Rattanaumpawan Pinyo , Luvira Viravarn , Chongtrakool Piriyaporn , Kaewkungwal Jaranit , Chokephaibulkit Kulkanya , Phisalprapa Pochamana
Published: June 2023

Introduction

This study aims to assess the economic impact of introducing the 13-valent pneumococcal conjugate vaccine (PCV13) and 23-valent pneumococcal polysaccharide vaccine (PPSV23) to Thai older adult aged ≥ 65  years who are healthy or with chronic health conditions and immunocompromised conditions from a societal perspective in order to introduce the vaccine to Thailand’s National Immunization Program for the older adult.

Methods

A Markov model was adopted to simulate the natural history and economic outcomes of invasive pneumococcal diseases using updated published sources and Thai databases. We reported analyses as incremental cost-effectiveness ratios (ICER) in USD per quality-adjusted life year (QALY) gained. In addition, sensitivity analyses and budget impact analyses were conducted.

Results

The base-case analysis of all interventions (no vaccinations [current standard of care in Thailand], PPSV23, and PCV13) showed that PPSV23 was extendedly dominated by PCV13. Among healthy individuals or those with chronic health conditions, ICER for PCV13 was 233.63 USD/QALY; meanwhile, among individuals with immunocompromised conditions, ICER for PCV13 was 627.24 USD/QALY. PCV13 are economical vaccine for all older adult Thai individuals when compared to all interventions.

Conclusions

In the context of Thailand, PCV13 is recommended as the best buy and should be primarily prioritized when both costs and benefits are considered. Also, this model will be beneficial to the two-next generation pneumococcal vaccines implementation in Thailand.
Early-onset group B streptococcal disease in African countries and maternal vaccination strategies
Authors: Dangor Ziyaad , Seale Anna C. , Baba Vuyelwa , Kwatra Gaurav
Published: June 2023

Invasive group B streptococcal (GBS) disease is the commonest perinatally-acquired bacterial infection in newborns; the burden is higher in African countries where intrapartum antibiotic prophylaxis strategies are not feasible. In sub-Saharan Africa, almost one in four newborns with GBS early-onset disease will demise, and one in ten survivors have moderate or severe neurodevelopmental impairment. A maternal GBS vaccine to prevent invasive GBS disease in infancy is a pragmatic and cost-effective preventative strategy for Africa. Hexavalent polysaccharide protein conjugate and Alpha family surface protein vaccines are undergoing phase II clinical trials. Vaccine licensure may be facilitated by demonstrating safety and immunological correlates/thresholds suggestive of protection against invasive GBS disease. This will then be followed by phase IV effectiveness studies to assess the burden of GBS vaccine preventable disease, including the effect on all-cause neonatal infections, neonatal deaths and stillbirths.