bluuebunny/crossref_metadata_2025 huggingface dataset:
Employing fasting plasma glucose to safely limit the use of oral glucose tolerance tests in pregnancy: a pooled analysis of four Norwegian studies
Authors: Rai Anam Shakil , Sletner Line , Jenum Anne Karen , Øverby Nina Cecilie , Stafne Signe Nilssen , Qvigstad Elisabeth , Pripp Are Hugo , Sagedal Linda Reme
Published: November 2023

Background/objective

There is no international consensus about the optimal approach to screening and diagnosis of gestational diabetes mellitus (GDM). Fasting plasma glucose (FPG) has been proposed as an alternative universal screening test to simplify the diagnosis of GDM. We investigate the ability of the FPG to predict a 2-hour glucose value below the cut-off for GDM, thereby “ruling out” the necessity of a full OGTT and assess the proportion of GDM-related complications associated with the identified FPG level.

Materials and methods

This study included secondary data from four Norwegian pregnancy cohorts (2002-2013), encompassing 2960 women universally screened with late mid-pregnancy 75g OGTT measuring FPG and 2-hour glucose. For a range of FPG thresholds, we calculated sensitivity to predict elevated 2-hour glucose, number of OGTTs needed and percentage of GDM cases missed, applying modified World Health Organization (WHO) 2013 criteria (2013WHO) and 2017 Norwegian criteria (2017Norwegian). We analyzed pregnancy outcomes for women above and below our selected threshold.

Results

The prevalence of GDM was 16.6% (2013WHO) and 10.1% (2017Norwegian). A FPG threshold of 4.7 mmol/L had a sensitivity of 76% (2013WHO) and 80% (2017Norwegian) for detecting elevated 2-hour glucose, with few missed GDM cases (2.0% of those ruled out and 7.5% of all GDM cases for 2013WHO, and 1.1% of those ruled out and 7% of all GDM cases for 2017Norwegian). When excluding women with FPG <4.7mmol/l and those with GDM based on FPG, only 24% (2013WHO) and 29% (2017Norwegian) would require OGTT. Women with FPG <4.7mmol/l, including missed GDM cases, had low risk of large-for-gestational-age newborns, cesarean section and operative vaginal delivery.

Conclusion

A FPG threshold of 4.7mmol/l as a first step when screening for GDM could potentially eliminate the need for OGTT in 70-77% of pregnancies. Women with FPG below this threshold appear to carry low risk of GDM-associated adverse pregnancy outcomes.
Research on the fugitive soil dust emission inventory in Western China based on wind erosion equation parameter optimization
Authors: Wang Pengbo , Chen Min , An Weiming , Liu Yongle , Pan Feng
Published: November 2023

The Wind Erosion Equation, currently one of the primary methods for estimating fugitive soil dust emission inventory, is influenced by several factors. Taking the convergent areas of the Tibet Plateau, Loess Plateau, and Qinba Mountains in Western China, we have optimized the climate factor using the WRF model driven by ERA5 reanalysis data. Additionally, we have modified the vegetation cover factors via normalized difference vegetation index and considered the impacts of the land use and cover change. Subsequently, other factors were allocated utilizing geographic information system, and the grid-based fugitive soil dust emission inventory for the study area for 2019 was derived through calculation. Based on the climate factor and vegetation cover factor, we have come up with the monthly allocation coefficients. The study has revealed the following findings: (1) Climate factors are unevenly distributed throughout the focused region, with the Loess Plateau showing the highest value, followed by the Tibet Plateau and the Qinba Mountains. There are also significant variations in the distribution of these factors among municipalities and counties; (2) The order of vegetation cover factor, primarily influenced by regional background as well as agricultural and pastoral activities, in the Loess Plateau, Tibetan Plateau and Qinba Mountains, is consistent with that of the wind erosion index; (3) In 2019, fugitive dust emissions from total suspended particles, PM10, and PM2.5 reached 9835.9, 2950.8, and 491.8 kt/a, respectively. The Loess Plateau exhibited the highest emission intensity due to factors such as low vegetation coverage, precipitation, high wind speed and wind erosion index; (4) Climate factor and vegetation cover factor are the primary factors influencing the monthly allocation coefficients. In 2019, the highest monthly fugitive dust emissions were estimated in April, accounting for approximately 36.21% of the total. The second and third-highest were found in August and June, respectively. This phenomenon can be explained climatically, as the Loess Plateau, semi-arid and arid regions, did not experience a significant increase in rainfall corresponding to rising temperatures.
Analysis of methods for assimilating fire perimeters into a coupled fire-atmosphere model
Authors: Kochanski Adam K. , Clough Kathleen , Farguell Angel , Mallia Derek V. , Mandel Jan , Hilburn Kyle
Published: November 2023

Correctly initializing the fire within coupled fire-atmosphere models is critical for producing accurate forecasts of meteorology near the fire, as well as the fire growth, and plume evolution. Improperly initializing the fire in a coupled fire-atmosphere model can introduce forecast errors that can impact wind circulations surrounding the fire and updrafts along the fire front. A well-constructed fire initialization process must be integrated within coupled fire-atmosphere models to ensure that the atmospheric component of the model does not become numerically unstable due to excessive heat fluxes released during the ignition, and that realistic fire-induced atmospheric circulations are established at the model initialization time. The primary objective of this study is to establish an effective fire initialization method in a coupled fire-atmosphere model, based on the analysis of the impact of the initialization procedure on the model’s ability to resolve fire-atmosphere circulations and fire growth. Here, we test three different fire initialization approaches leveraging the FireFlux II experimental fire, which provides a comprehensive suite of observations of the pyroconvective column, local micrometeorology, and fire characteristics. The two most effective fire initialization methods identified using the FireFlux II case study are then tested on the 380,000-acre Creek Fire, which burned across the central Sierra Nevada mountains during the 2020 Western U.S. wildfire season. For this case study, simulated pyroconvection and fire progression are evaluated using plume top height observations from MISR and airborne fire perimeter data, to assess the effectiveness of different initialization methods in the context of establishing pyroconvection and resolving the fire growth. The analyses of both the experimental fire simulation and the wildfire simulation indicate that the spin-up initialization method based on historical fire progression that masks out inactive fire regions provides the best results in terms of resolving the fire-induced vertical circulation and fire progression.
Case report: Revealing the rare—a Brody Disease patient from Turkey expanding the phenotype
Authors: Şahin Ayça , Badakal Esmer Zeynep Duru , Kovancılar Koç Müge , Uysal Hilmi , Başak Ayşe Nazlı
Published: November 2023

Brody Disease is an exceptionally rare, autosomal recessive myopathy attributed to the pathogenic variants in the ATP2A1, which encodes the sarcoplasmic/endoplasmic reticulum Ca (2+) ATPase type 1 protein SERCA1. It was first described by Brody IA in 1969. To date, only thirty-three Brody families with forty-seven patients have been reported in the literature, and the disease prevalence is considered as 1 in 10 million, demonstrating the peculiarity of the disease. Clinical characteristics of Brody Disease include muscle stiffness after exercise, myalgia, and muscle cramps. Brody Disease patients generally have disease onset in the first decade, and genetic diagnosis is delayed as a consequence of both the rareness and the mild course of the disease. Here, we report a Turkish Brody Disease patient with a homozygous c.428G>A p.Arg143Gln (NM_004320.4) missense mutation in the ATP2A1. The male patient, whose symptoms started at the age of 14–15, is now 36 years old. His clinical manifestations are athletic appearance, exotropia, slightly elevated creatine kinase (CK), mild progressive proximal muscle weakness in the lower extremities, muscle cramps, pain and stiffness. The patient described here has a very mild progression with an onset in the second decade, expanding the Brody Disease phenotype. The study also implies that in the era of emerging genetic therapies, the routine testing of patients with myopathies is a prerequisite since not only future therapies will be designed on molecular findings, but also currently available symptomatic and palliative treatment options will be more precisely applied.
Leishmania infection upregulates and engages host macrophage Argonaute 1, and system-wide proteomics reveals Argonaute 1-dependent host response
Authors: Moradimotlagh Atieh , Chen Stella , Koohbor Sara , Moon Kyung-Mee , Foster Leonard J. , Reiner Neil , Nandan Devki
Published: November 2023

Leishmania donovani, an intracellular protozoan parasite, is the causative agent of visceral leishmaniasis, the most severe form of leishmaniasis in humans. It is becoming increasingly clear that several intracellular pathogens target host cell RNA interference (RNAi) pathways to promote their survival. Complexes of Argonaute proteins with small RNAs are core components of the RNAi. In this study, we investigated the potential role of host macrophage Argonautes in Leishmania pathogenesis. Using Western blot analysis of Leishmania donovani-infected macrophages, we show here that Leishmania infection selectively increased the abundance of host Argonaute 1 (Ago1). This increased abundance of Ago1 in infected cells also resulted in higher levels of Ago1 in active Ago-complexes, suggesting the preferred use of Ago1 in RNAi in Leishmania-infected cells. This analysis used a short trinucleotide repeat containing 6 (TNRC6)/glycine-tryptophan repeat protein (GW182) protein-derived peptide fused to Glutathione S-transferase as an affinity matrix to capture mature Ago-small RNAs complexes from the cytosol of non-infected and Leishmania-infected cells. Furthermore, Ago1 silencing significantly reduced intracellular survival of Leishmania, demonstrating that Ago1 is essential for Leishmania pathogenesis. To investigate the role of host Ago1 in Leishmania pathogenesis, a quantitative whole proteome approach was employed, which showed that expression of several previously reported Leishmania pathogenesis-related proteins was dependent on the level of macrophage Ago1. Together, these findings identify Ago1 as the preferred Argonaute of RNAi machinery in infected cells and a novel and essential virulence factor by proxy that promotes Leishmania survival.
Boolean model of the gene regulatory network of Pseudomonas aeruginosa CCBH4851
Authors: Chagas Márcia da Silva , Trindade dos Santos Marcelo , Argollo de Menezes Marcio , da Silva Fabricio Alves Barbosa
Published: November 2023

Introduction

Pseudomonas aeruginosa infections are one of the leading causes of death in immunocompromised patients with cystic fibrosis, diabetes, and lung diseases such as pneumonia and bronchiectasis. Furthermore, P. aeruginosa is one of the main multidrug-resistant bacteria responsible for nosocomial infections worldwide, including the multidrug-resistant CCBH4851 strain isolated in Brazil.

Methods

One way to analyze their dynamic cellular behavior is through computational modeling of the gene regulatory network, which represents interactions between regulatory genes and their targets. For this purpose, Boolean models are important predictive tools to analyze these interactions. They are one of the most commonly used methods for studying complex dynamic behavior in biological systems.

Results and discussion

Therefore, this research consists of building a Boolean model of the gene regulatory network of P. aeruginosa CCBH4851 using data from RNA-seq experiments. Next, the basins of attraction are estimated, as these regions and the transitions between them can help identify the attractors, representing long-term behavior in the Boolean model. The essential genes of the basins were associated with the phenotypes of the bacteria for two conditions: biofilm formation and polymyxin B treatment. Overall, the Boolean model and the analysis method proposed in this work can identify promising control actions and indicate potential therapeutic targets, which can help pinpoint new drugs and intervention strategies.
A cooperativity between virus and bacteria during respiratory infections
Authors: Lalbiaktluangi C. , Yadav Mukesh Kumar , Singh Prashant Kumar , Singh Amit , Iyer Mahalaxmi , Vellingiri Balachandar , Zomuansangi Ruth , Zothanpuia  , Ram Heera
Published: November 2023

Respiratory tract infections remain the leading cause of morbidity and mortality worldwide. The burden is further increased by polymicrobial infection or viral and bacterial co-infection, often exacerbating the existing condition. Way back in 1918, high morbidity due to secondary pneumonia caused by bacterial infection was known, and a similar phenomenon was observed during the recent COVID-19 pandemic in which secondary bacterial infection worsens the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) condition. It has been observed that viruses paved the way for subsequent bacterial infection; similarly, bacteria have also been found to aid in viral infection. Viruses elevate bacterial infection by impairing the host’s immune response, disrupting epithelial barrier integrity, expression of surface receptors and adhesion proteins, direct binding of virus to bacteria, altering nutritional immunity, and effecting the bacterial biofilm. Similarly, the bacteria enhance viral infection by altering the host’s immune response, up-regulation of adhesion proteins, and activation of viral proteins. During co-infection, respiratory bacterial and viral pathogens were found to adapt and co-exist in the airways of their survival and to benefit from each other, i.e., there is a cooperative existence between the two. This review comprehensively reviews the mechanisms involved in the synergistic/cooperativity relationship between viruses and bacteria and their interaction in clinically relevant respiratory infections.
Maize-soybean intercropping improved maize growth traits by increasing soil nutrients and reducing plant pathogen abundance
Authors: Liu Meiyu , Zhao Huicheng
Published: November 2023

Introduction

Maize (Zea mays L.)–soybean (Glycine max L.) intercropping has been widely utilized in agricultural production due to its effectiveness in improving crop yield and nutrient use efficiency. However, the responses of maize rhizosphere microbial communities and the plant pathogen relative abundance to maize growth traits in maize-soybean intercropping systems with different chemical nitrogen fertilizer application rates remain unclear.

Methods

In this study, a field experiment was conducted, and the bacterial and fungal communities of maize rhizosphere soils in maize-soybean intercropping systems treated with different N fertilization rates were investigated using Illumina NovaSeq sequencing. Maize growth traits, soil physicochemical properties and soil enzyme activities were also examined.

Results and discussion:

We found that intercropping and N fertilizer treatments strongly influenced soil microbial diversity, structure and function. The PLSPM (partial least squares path modeling) confirmed that soil nutrients directly positively affected maize biomass and that intercropping practices indirectly positively affected maize biomass via soil nutrients, especially NH4+-N. Intercropping agronomic approaches also improved maize growth traits by reducing the plant pathogen abundance, and the relative abundance of the plant pathogen Trichothecium roseum significantly decreased with intercropping treatments compared to monocropping treatments. These results confirmed the benefits of maize-soybean intercropping treatments for agricultural production.
Bacteroides acidifaciens and its derived extracellular vesicles improve DSS-induced colitis
Authors: Zheng Cihua , Zhong Yuchun , Xie Jian , Wang Zhuoya , Zhang Wenming , Pi Yiming , Zhang Wenjun , Liu Li , Luo Jun , Xu Wei
Published: November 2023

Introduction

“Probiotic therapy” to regulate gut microbiota and intervene in intestinal diseases such as inflammatory bowel disease (IBD) has become a research hotspot. Bacteroides acidifaciens, as a new generation of probiotics, has shown beneficial effects on various diseases.

Methods

In this study, we utilized a mouse colitis model induced by dextran sodium sulfate (DSS) to investigate how B. acidifaciens positively affects IBD. We evaluated the effects ofB. acidifaciens, fecal microbiota transplantation, and bacterial extracellular vesicles (EVs) on DSS-induced colitis in mice. We monitored the phenotype of mouse colitis, detected serum inflammatory factors using ELISA, evaluated intestinal mucosal barrier function using Western blotting and tissue staining, evaluated gut microbiota using 16S rRNA sequencing, and analyzed differences in EVs protein composition derived from B. acidifaciens using proteomics to explore how B. acidifaciens has a positive impact on mouse colitis.

Results

We confirmed that B. acidifaciens has a protective effect on colitis, including alleviating the colitis phenotype, reducing inflammatory response, and improving intestinal barrier function, accompanied by an increase in the relative abundance of B. acidifaciens and Ruminococcus callidus but a decrease in the relative abundance of B. fragilis. Further fecal bacterial transplantation or fecal filtrate transplantation confirmed the protective effect of eosinophil-regulated gut microbiota and metabolites on DSS-induced colitis. Finally, we validated that EVs derived from B. acidifaciens contain rich functional proteins that can contribute to the relief of colitis.

Conclusion

Therefore, B. acidifaciens and its derived EVs can alleviate DSS-induced colitis by reducing mucosal damage to colon tissue, reducing inflammatory response, promoting mucosal barrier repair, restoring gut microbiota diversity, and restoring gut microbiota balance in mice. The results of this study provide a theoretical basis for the preclinical application of the new generation of probiotics.
Deep brain stimulation for chronic pain: a systematic review and meta-analysis
Authors: Shaheen Nour , Shaheen Ahmed , Elgendy Abdelrahman , Bezchlibnyk Yarema B. , Zesiewicz Theresa , Dalm Brian , Jain Jennifer , Green Alexander L. , Aziz Tipu Z. , Flouty Oliver
Published: November 2023

Background

Deep brain stimulation (DBS) has shown promise in effectively treating chronic pain. This study aimed to assess the efficacy of DBS in this context.

Methods

We conducted a systematic literature search using PubMed, Scopus, and Web of Science, following the PRISMA guidelines. A well-constructed search strategy was utilized. Our literature search identified two groups of subjects: one group underwent DBS specifically for chronic pain treatment (DBS-P), while the second group received DBS for other indications (DBS-O), such as Parkinson’s disease or dystonia, with pain perception investigated as a secondary outcome in this population. Meta-analysis was performed using R version 4.2.3 software. Heterogeneity was assessed using the tau^2 and I^2 indices, and Cochran’s Q-test was conducted.

Results

The analysis included 966 patients in 43 original research studies with chronic pain who underwent DBS (340 for DBS-P and 625 for DBS-O). Subgroup analysis revealed that DBS-P exhibited a significant effect on chronic pain relief, with a standardized mean difference (SMD) of 1.65 and a 95% confidence interval (CI) of [1.31; 2.00]. Significant heterogeneity was observed among the studies, with an I^2 value of 85.8%. However, no significant difference was found between DBS-P and DBS-O subgroups. Subgroup analyses based on study design, age, pain diseases, and brain targets demonstrated varying levels of evidence for the effectiveness of DBS across different subgroups. Additionally, meta-regression analyses showed no significant relationship between age or pain duration and DBS effectiveness for chronic pain.

Conclusion

These findings significantly contribute to the expanding body of knowledge regarding the utility of DBS in the management of chronic pain. The study underscores the importance of conducting further research to enhance treatment outcomes and elucidate patient-specific factors that are associated with treatment response.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=428442, identifier CRD42023428442.
Serum exosomal circCCDC66 as a potential diagnostic and prognostic biomarker for pituitary adenomas
Authors: Yue Xiao , Lan Fengming , Liu Weiping
Published: November 2023

Purpose

Circular RNAs (circRNAs) play an important role in tumorigenesis, and exosomal circRNAs have the potential to be novel biomarkers for cancer diagnosis. Here, we are committed to reveal serum exosomal circCCDC66 as a noninvasive biomarker to diagnose and predict recurrence in pituitary adenoma (PA).

Methods

A total of 90 PA patients and 50 healthy subjects were enrolled for clinical validation. Exosomes were extracted from the serum and validated by transmission electron microscopy, nanoparticle tracking analysis, and Western blot assay. The expression of circCCDC66 in serum exosomes was assessed using quantitative real-time PCR (qRT-PCR), and correlations between circCCDC66 expression and clinicopathological factors were analyzed. The reliability of serum exosomal circCCDC66 to diagnose PA was evaluated using receiver operating characteristic (ROC) analysis.

Results

Initially, an obviously significantly increasing level of serum exosomal circCCDC66 was verified in the PA specimens compared with healthy controls. Importantly, serum exosomal circCCDC66, which was secreted and released by PA cells, could monitor tumor dynamics and serve as a potentially prognostic biomarker during treatment. Additionally, ROC curve analysis was performed and the corresponding area under the curve (AUC) values were used to confirm the ability of serum exosomal circCCDC66 as a potentially diagnostic and prognostic biomarker for PA patients. Importantly, the progression-free survival was much longer in the low serum exosomal circCCDC66 group than in the high serum exosomal circCCDC66 group.

Conclusion

Serum exosomal circCCDC66 is abnormally elevated and may serve as a promising factor for the diagnosis of and predicting prognosis in PA patients.
Pulmonary infection associated with immune dysfunction is associated with poor prognosis in patients with myelodysplastic syndrome accompanied by TP53 abnormalities
Authors: Chen Yi , Zheng Jing , Qiu Yanyan , Wu Zhengjun , Luo Xiaofeng , Zhu Liangfang , Wu Yong , Lin Yanjuan
Published: November 2023

The aim of this study was to examine the characteristics and prognosis of patients with myelodysplastic syndrome (MDS) accompanied by TP53 abnormalities and explore potential prognostic factors and treatment responses. This retrospective analysis included 95 patients with MDS and TP53 abnormalities and 173 patients with MDS without TP53 abnormalities at the Fujian Medical University Union Hospital between January 2016 and June 2023. Among patients with TP53 abnormalities, 26 (27.4%) developed AML during the disease course, with a median transformation time of 5.7 months. Complex karyotypes were observed in 73.1% of patients, and the proportions of -5 or del(5q), -7 or del(7q), +8, and -20 or del(20q) were 81.8%, 54.5%, 30.7%, and 25.0%, respectively. These patients exhibited poor survival, with a median overall survival (OS) of 7.3 months, and had 1- and 2-year OS rates of 42.2% and 21.5%, respectively. The complete response rates for azacitidine monotherapy, venetoclax combined with azacitidine, decitabine monotherapy, and decitabine combined with low-dose chemotherapy were 9.1%, 41.7%, 37.5%, and 33.3%, respectively. Long-term survival was similar among the four treatment groups. Patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT) had a median OS of 21.3 months, which trended to be longer than that of patients who did not undergo allo-HSCT (5.6 months; P = 0.1449). Patients with pulmonary infection at diagnosis experienced worse OS than those without pulmonary infection (2.3 months vs. 15.4 months; P < 0.0001). Moreover, 61.9% of patients with pulmonary infection had immune dysfunction, with a ratio of CD4+ to CD8+ T lymphocytes below two. Pulmonary infections and complex karyotypes were independent adverse prognostic factors for OS. In conclusion, TP53 abnormalities in patients with MDS were frequently accompanied by complex karyotypes, and treatments based on hypomethylating agents or venetoclax have limited efficacy. Pulmonary infections associated with immune dysfunction is associated with poor prognosis.
Global research trends and hot spots on autophagy and kidney diseases: a bibliometric analysis from 2000 to 2022
Authors: Ai Sinan , Li Yake , Zheng Huijuan , Wang Zhen , Liu Weijing , Tao JiaYin , Li Yaotan , Wang Yaoxian
Published: November 2023

Background: Autophagy is an essential cellular process involving the self-degradation and recycling of organelles, proteins, and cellular debris. Recent research has shown that autophagy plays a significant role in the occurrence and development of kidney diseases. However, there is a lack of bibliometric analysis regarding the relationship between autophagy and kidney diseases.

Methods: A bibliometric analysis was conducted by searching for literature related to autophagy and kidney diseases in the Web of Science Core Collection (WoSCC) database from 2000 to 2022. Data processing was carried out using R package “Bibliometrix”, VOSviewers, and CiteSpace.

Results: A total of 4,579 articles related to autophagy and kidney diseases were collected from various countries. China and the United States were the main countries contributing to the publications. The number of publications in this field showed a year-on-year increasing trend, with open-access journals playing a major role in driving the literature output. Nanjing Medical University in China, Osaka University in Japan, and the University of Pittsburgh in the United States were the main research institutions. The journal “International journal of molecular sciences” had the highest number of publications, while “Autophagy” was the most influential journal in the field. These articles were authored by 18,583 individuals, with Dong, Zheng; Koya, Daisuke; and Kume, Shinji being the most prolific authors, and Dong, Zheng being the most frequently co-cited author. Research on autophagy mainly focused on diabetic kidney diseases, acute kidney injury, and chronic kidney disease. “Autophagy”, “apoptosis”, and “oxidative stress” were the primary research hotspots. Topics such as “diabetic kidney diseases”, “sepsis”, “ferroptosis”, “nrf2”, “hypertension” and “pi3k” may represent potential future development trends. Research on autophagy has gradually focused on metabolic-related kidney diseases such as diabetic nephropathy and hypertension. Additionally, PI3K, NRF2, and ferroptosis have been recent research directions in the field of autophagy mechanisms.

Conclusion: This is the first comprehensive bibliometric study summarizing the relationship between autophagy and kidney diseases. The findings aid in identifying recent research frontiers and hot topics, providing valuable references for scholars investigating the role of autophagy in kidney diseases.
Effect of Lactobacillus plantarum PS128 on neuromuscular efficiency after a half-marathon
Authors: Yu Chia-Hsien , Lai Chang-Chi , Chen Jing-Hsuan , Chen I-Cheng , Tai Hsia-Ling , Fu Szu-Kai
Published: November 2023

Introduction:Lactobacillus plantarum PS128 (PS128) could be considered an antioxidant supplement to reduce muscle fatigue and improve exercise capacity recovery after vigorous exercise.

Purpose: The purpose of this study is to investigate the effect of PS128 on muscle fatigue and electromyography (EMG) activity after a half-marathon (HM).

Methods: The experimental design used a repeated-measures design with a double-blind approach. The participants either took two capsules of PS128 for 4 weeks as the PS128 group (PSG, n = 8) or took two capsules of a placebo for 4 weeks as the placebo group (PLG, n = 8) to ensure counterbalancing. The time points of the maximal voluntary isometric contraction (MVIC) and EMG activity test were set before probiotics were taken (baseline), 48 h before HM (Pre), and immediately at 0 h, 3 h, 24 h, 48 h, 72 h, and 96 h after HM.

Results: EMG activity included median power frequency (MDF), integrated EMG (iEMG), and neuromuscular efficiency (peak torque/iEMG). The MVICs of knee extensors, analyzed by using an isokinetic dynamometer, showed a decrease from the Pre to 0 h (p = 0.0001), 3 h (p < 0.0001), 24 h (p < 0.0001), 48 h (p < 0.0001), 72 h (p = 0.0002), and 96 h (p = 0.0408) time points in the PLG. Sidak’s multiple comparisons tests showed that the PLG was significantly lower than the PSG at 0 h (p = 0.0173), 3 h (p < 0.0001), 24 h (p < 0.0001), 48 h (p < 0.0001), 72 h (p < 0.0001), and 96 h (p = 0.0004) time points. The MDF of vastus medialis oblique (VMO) in the PLG was significantly decreased 24 h after HM and significantly lower than that in the PSG at all times points after HM. The iEMG of VMO in the PLG was significantly decreased 48 h after HM and significantly lower than that in the PSG at 0 h, 3 h, 24 h, 48 h, and 72 h after HM.

Conclusion: The PS128 supplementation may prevent the decrease in MDF, iEMG, and peak torque after vigorous exercise. Recreational runners may consider implementing a probiotic supplementation regimen as a potential strategy to mitigate muscle fatigue following HM.
Geographically associated endophytic fungi contribute to the tropane alkaloids accumulation of Anisodus tanguticus
Authors: Wang Bo , Chen Chen , Xiao Yuanming , He Yan , Gao Ying , Kang Zongxiu , Wei Xiaoxuan , Deng Yujie , Feng Shihong , Zhou Guoying
Published: November 2023

Anisodus tanguticus is a valuable plant for extracting tropane alkaloids. However, the mechanisms by which plant microbiome mediate the accumulation of tropane alkaloids in Anisodus tanguticus are still not well understood. In this study, we collected 55 wild Anisodus tanguticus populations on the Tibetan Plateau and the tropane alkaloids content, and root-related bacteria and fungi diversity were analyzed using HPLC and 16 s rDNA and ITS sequencing. The results showed that tropane alkaloids content has obvious geographical distribution characteristics. Anisodine content had a significant positive correlation with latitude, while anisodamine and atropine content had a significant negative correlation with latitude. Variation partition analysis (VPA) showed that root endophytes play a significant role in promoting tropane alkaloid production in Anisodus tanguticus roots. The root endophytes alone explained 14% of the variation, which was the largest contributor. Soil properties variables could independently explain 5% of the variation, and climate variables could explain 1% of the variation. Of these, endophytic fungi alone accounted for 11%, while bacteria explained only 5%. Random forests and Mantel test showed that different regionally enriched endophytic fungi have a greater impact on the accumulation of tropane alkaloids than the whole endophytic fungi. Richness and relative abundance of enriched endophytic fungi in Hengduan-Qilian Mountains (HQ) group has a significant positive correlation with anisodine content, while richness and relative abundance of enriched endophytic fungi in Himalayas-Hengduan Mountains (HH) group has a significant positive correlation with anisodamine and atropine content. And, these enriched endophytic fungi have high network connectivity and distributed in separate network modules. This study further confirmed that endophytes were closely related to tropane alkaloids accumulation in Anisodus tanguticus and contribute to promote sustainable development, cultivation, and precision medicine of Anisodus tanguticus.
Cognitive flexibility and entrepreneurial creativity: the chain mediating effect of entrepreneurial alertness and entrepreneurial self-efficacy
Authors: Yu Xiaoling , Zhao Xiuli , Hou Yongxiong
Published: November 2023

Entrepreneurial creativity is an important part of entrepreneurs’ competency structure, and studies have been conducted to explore its impact on outcome variables like entrepreneurial performance, etc., but there are fewer studies on its antecedent variables at the individual level. In the relational model of entrepreneurial creativity, cognitive flexibility, entrepreneurial alertness, and entrepreneurial self-efficacy are included to construct a mediated use spss26.0 model, and data are collected from a research sample of 325 entrepreneurs for empirical analysis. The results indicate that: cognitive flexibility has a positive effect on entrepreneurial creativity; entrepreneurial alertness plays an independent mediating role between cognitive flexibility and entrepreneurial creativity, which is similar to how entrepreneurial self-efficacy plays the role between cognitive flexibility and creativity; entrepreneurial alertness and entrepreneurial self-efficacy then play a chain mediating role between cognitive flexibility and creativity. These findings reveal that three variables jointly influence entrepreneurial creativity, providing new theoretical and practical insights for understanding and enhancing entrepreneurial creativity. In addition, the study provides valuable guidance for entrepreneurship education and training, which can help entrepreneurs to better utilize their creativity and thus promote innovation and success in entrepreneurial activities.
Development and content validity of the Person Experiences Interview Survey (PEIS): a measure of the mental health services experiences of people with developmental disabilities
Authors: Kramer Jessica M. , Beasley Joan B. , Caoili Andrea , Kalb Luke , Urquilla Micah Peace , Klein Ann E. , Poncelet Janie , Black Sandra , Tessler Richard C.
Published: November 2023

Purpose

People with developmental disabilities and mental health service experiences have a right to be included in healthcare decisions, including the evaluation of their mental health services and providers. However, few self-report measures address this need. This study aimed to fill this gap by developing and evaluating the content validity, including comprehension, relevance, and comprehensiveness, of the Person Experiences Interview Survey (PEIS) with people with developmental disabilities and mental health experiences.

Methods

The research team established a measurement framework based on the Family Experiences Interview Survey (FEIS), resulting in 21 PEIS items that were written in collaboration with young adults with developmental disabilities and mental health service experiences. Comprehension, relevance, and comprehensiveness were evaluated through cognitive interviews with people with developmental disabilities and mental health service experiences (respondents; n = 9) ages 23–49 years. Comprehensiveness and relevance were also evaluated in focus groups with family caregivers (n = 9) and mental health providers (n = 10) who serve this population. Two researchers independently coded open-ended responses to the PEIS for comprehension. A content validity index (CVI), indicating relevance, was calculated for each participant group for each item, and comprehensiveness was rated for item sets.

Results

Fifteen of the 21 items met the criteria of ≥80% comprehension, with 89–100% of responses containing all or some intended information. All items met the CVI ≥80% criterion in at least two of the three groups. In all item sets, between 1 and 4 family members or providers felt one question was missing. Respondents used the response scale in a manner that corresponded with their open-ended descriptions, and family caregivers and providers had positive feedback about the response scale’s visual cues and number of choices. Using these findings, four items were removed and six items were revised, resulting in a 17-item measure.

Conclusion

This study presents a novel and promising measure, the Person Experiences Interview Survey (PEIS). It also demonstrates that the employment of accessible methods allows people with developmental disabilities to meaningfully evaluate mental health services and providers. The PEIS shows great promise for application in the field by engaging those directly involved in the evaluation of mental health services and providers.
The relevance of oral exposure in the workplace: a systematic review and meta-analysis
Authors: Dietz Marlene , Schnieder Wiebke Ella , Schlüter Urs , Kahl Anke
Published: November 2023

Introduction

The inclusion of all relevant exposure routes in the exposure assessment is essential for the protection of workers. However, under European chemical regulations but also for workplace risk assessments according to occupational safety and health (OSH) requirements, the quantitative assessment of oral exposure is usually neglected assuming good occupational hygiene. In contrast, several studies point to the importance of unintentional ingestion in the workplace. To our knowledge, there is no systematic analysis of the extent of this exposure route.

Methods

Therefore, the aim of this study was to assess systematically the current knowledge on the relevance of occupational oral exposure using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) method. Five electronic databases and nine institutional websites were searched for all publications on the relevance. The data were extracted into a concept matrix. In the subsequent meta-analysis, the identified conclusions on the relevance were analyzed. In addition, the measurement methods or modeling approaches that were described for occupational oral exposure were determined as well as the potentially relevant workplaces and substances.

Results

In total, 147 studies were included in this analysis that contain a general or several, differentiated assessments of the relevance of occupational oral exposure. Nine of these studies assessed this exposure route as irrelevant. However, 123 studies considered oral exposure as potentially contributing and 80 studies explicitly identified it as relevant. 78 and 94 of the publications described modeling and measurement approaches, respectively. The workplaces frequently identified as potentially or explicitly relevant were other indoor, other industrial or recycling workplaces. Analogously, metals, dust and powders or pesticides were the most frequently investigated substance groups.

Discussion

As several studies assessed occupational oral exposure as relevant in the context of different workplaces and substances, further investigation of this exposure route is needed. This systematic review and meta-analysis serve as a basis for further development of feasible assessment methods for this route of exposure.
Research on Friction Compensation Method of Electromechanical Actuator Based on Improved Active Disturbance Rejection Control
Authors: Zhang Pan , Shi Zhaoyao , Yu Bo
Published: November 2023

The friction factor of harmonic reducers affects the transmission accuracy in electromechanical actuators (EMAs). In this study, we proposed a friction feedforward compensation method based on improved active disturbance rejection control (IADRC). A mathematical model of EMA was developed. The relationship between friction torque and torque current was derived. Furthermore, the compound ADRC control method of second-order speed loop and position loop was studied, and an IADRC control method was proposed. A real EMA was developed, and the working principles of the EMA driving circuit and current sampling were analyzed. The three methods—PI, ADRC, and IADRC—were verified by conducting speed step experiments and sinusoidal tracking experiments. The integral values of time multiplied by the absolute error of the three control modes under the step speed mode were approximately 47.7, 32.1, and 15.5, respectively. Disregarding the inertia of the reducer and assuming that the torque during no-load operation equals the friction torque during constant motion, the findings indicate that, under a load purely driven by inertia, the IADRC control method enhances tracking accuracy.
Design and Validation of a Variable-Rate Control Metering Mechanism and Smart Monitoring System for a High-Precision Sugarcane Transplanter
Authors: Elwakeel Abdallah E. , Mazrou Yasser S. A. , Eissa Ahmed S. , Okasha Abdelaziz M. , Elmetwalli Adel H. , Makhlouf Abeer H. , Metwally Khaled A. , Mahmoud Wael A. , Elsayed Salah
Published: November 2023

The current study aimed to design and test the accuracy of a variable-rate control metering mechanism (VRCMM) and a remote smart monitoring system (RSMS) for a precision sugarcane transplanter based on IoT technology. The VRCMM is used to operate the seedling metering device at different speeds using a stepper motor based on the travel speed, and the RSMS was employed to evaluate of the three basic parameters of seedling amount, optimum rate, and missed rate. Two types of sensors were used for detecting the sugarcane seedling (SS) and travel speed, including one ultrasonic sensor and one infrared RPM sensor. The study was performed at five travel speeds and four transplant spacings. The findings of laboratory tests showed that the mean record of the relative error between the desired stepper motor speed of the VRCMM and the real value was 3.39%, and it increased with increasing the travel speed. as Additionally, the speed regulation performance was in agreement with the transplanting index. The change in RSMS accuracy is obvious when the travel speed is high and the transplant spacing is small. The RSMS accuracy drops sharply, revealing a leaping change. In conclusion, the smart and intelligent designed sugarcane transplanter would be very useful in sugarcane production.
Effects of Field Pea Diet and Immunocastration in Heavy Pigs on Fresh Pork and Dry-Cured Ham
Authors: Argemí-Armengol Immaculada , Tor Marc , Bottegal Diego , Latorre Maria A. , Serrano-Pérez Beatriz , Álvarez-Rodríguez Javier
Published: November 2023

Peas are an alternative to soybeans to supply protein in livestock feeds. Immunocastration avoids surgical castration of male pigs and increases fat deposition in female pigs. This work aimed to assess the effects of pea inclusion on the amount of fat and fatty acid profile of loins and on weight losses of dry-cured hams in different sexes of pigs; in addition, growth performance and feeding behavior in immunocastrated female pigs were evaluated. Two experiments were conducted with crossbred immunocastrated female (IF) and immunocastrated (IM) or surgically castrated (CM) male pigs from Duroc dams sired by Berkshire, which were assigned to one of two diets (soybean vs. pea-based). The effect of castration type on the afore-mentioned variables was evaluated in male pigs, and the growth performance, and feeding behavior in female pigs. The pea-based diet in IF had no effect on average daily gain nor on carcass traits; although, it increased feeding time (p < 0.001) and reduced n-3 polyunsaturated fatty acid (PUFA) content (p < 0.05). In male pigs, the pea-based diet did not change carcass fatness either but reduced the n-3 PUFA levels (p < 0.05). Likewise, IM had lower (p < 0.001) monounsaturated fatty acid (MUFA) and higher (p < 0.05) n-6 PUFA than CM. Diet did not affect dry-cured ham weight losses during the process, while IM showed greater (p < 0.001) losses than CM. Pigs fed a pea-based diet complied with the requirements of cured ham production, while immunocastration in male pigs increased weight losses, partly explained by lower fat content and higher fatty acid unsaturation.
Use of Biostimulants to Alleviate Anoxic Stress in Waterlogged Cabbage (Brassica oleracea var. capitata)—A Review
Authors: Buga Nadya , Petek Marko
Published: November 2023

In Europe, the intensity and frequency of flooding events are expected to increase due to climate change, adding additional challenges to agricultural production and creating the need for new plant products and adaptation tools. Cabbage is one economically important vegetable that is likely to be affected by increased flooding in Europe. This review investigates the potential of biostimulant applications based on algae extracts, amino acids, microorganisms, and nano-CaCO3 to assist cabbage plants subjected to waterlogged conditions. The overall findings from the studies reviewed indicate that these biostimulants could aid plants suffering from anoxic stress due to waterlogging through their ability to improve nutrient availability and plant nutrient status, modulate phytohormones and phytohormone signalling, increase compatible solutes, and enhance the antioxidant system. The effect of biostimulants is influenced by multiple factors; therefore, field studies are required to determine the most valuable biostimulant combination and application dose, type, and timing for cabbage, as well as any economic benefits that could arise. More precise information would benefit food producers by providing them with additional adaptation tools to use in a changing climate as well as natural products that are compatible with the agriculture sector’s transition to more sustainable and ecological management.
Infection Risk-Based Application of Plant Resistance Inducers for the Control of Downy and Powdery Mildews in Vineyards
Authors: Taibi Othmane , Fedele Giorgia , Salotti Irene , Rossi Vittorio
Published: November 2023

Plant resistance inducers (PRIs) are potential alternatives for controlling grapevine downy (DM) and powdery (PM) mildews in vineyards. In a 3-year field study, we evaluated the field efficacy of six commercial PRIs of chemical and natural origin against DM and PM diseases when applied at designated vine growth stages in a mixture with low doses of copper and sulfur, and only when advised by weather-driven disease models. The disease severity and incidence were evaluated for each season at key growth stages (i.e., the end of flowering, berries pea-sized, veraison, and pre-harvest), and areas under the disease progress curves (AUDPC) were calculated and compared with those of nontreated vines. These risk-based applications resulted in a 41% and 61% reduction of interventions against DM and PM, respectively, compared to the official advice for integrated pest management in the growing area. These applications provided a disease control efficacy of 88% for DM and 93% for PM; the disease severity on bunches never exceeded 5%. Overall, when the disease severity was expressed as AUDPC, we observed higher efficacy of all the PRIs for PM, and of laminarin and cerevisane for DM. We also found that potassium phosphonate and fosetyl-Al (commonly used against DM) were effective against PM, and cos-oga (used against PM) was effective against DM. These results broaden the application and integration of PRIs in viticulture.