bluuebunny/crossref_metadata_2025 huggingface dataset:
L-Cysteine Containing Vitamin Supplement Which Prevents or Alleviates Alcohol-related Hangover Symptoms: Nausea, Headache, Stress and Anxiety
Authors: Eriksson C J Peter , Metsälä Markus , Möykkynen Tommi , Mäkisalo Heikki , Kärkkäinen Olli , Palmén Maria , Salminen Joonas E , Kauhanen Jussi
Published: August 2020

Aims

Alcohol-related hangover symptoms: nausea, headache, stress and anxiety cause globally considerable amount of health problems and economic losses. Many of these harmful effects are produced by alcohol and its metabolite, acetaldehyde, which also is a common ingredient in alcohol beverages. The aim of the present study is to investigate the effect of the amino acid L-cysteine on the alcohol/acetaldehyde related aftereffects.

Methods

Voluntary healthy participants were recruited through advertisements. Volunteers had to have experience of hangover and/or headache. The hangover study was randomized, double-blind and placebo-controlled. Nineteen males randomly swallowed placebo and L-cysteine tablets. The alcohol dose was 1.5 g/kg, which was consumed during 3 h.

Results

The primary results based on correlational analysis showed that L-cysteine prevents or alleviates hangover, nausea, headache, stress and anxiety. For hangover, nausea and headache the results were apparent with the L-cysteine dose of 1200 mg and for stress and anxiety already with the dose of 600 mg.

Conclusions

L-cysteine would reduce the need of drinking the next day with no or less hangover symptoms: nausea, headache, stress and anxiety. Altogether, these effects of L-cysteine are unique and seem to have a future in preventing or alleviating these harmful symptoms as well as reducing the risk of alcohol addiction.
Phenomenal transparency and the transparency of subjecthood
Authors: Morris Kevin
Published: August 2020

According to phenomenal transparency, phenomenal concepts are transparent where a transparent concept is one that reveals the nature of that to which it refers. What is the connection between phenomenal transparency and our concept of a subject of experience? This paper focuses on a recent argument, due to Philip Goff, for thinking that phenomenal transparency entails transparency about subjecthood. The argument is premissed on the idea that subjecthood is related to specific phenomenal properties as a determinable of more specific determinates. I argue that the argument fails, which opens the door for one to endorse phenomenal transparency while denying transparency about the concept of a subject of experience. I draw out the consequences of this for certain versions of the combination problem for panpsychist metaphysics and moreover argue that rejecting the transparency of subjecthood does not undermine anti-physicalist arguments premissed on considerations relating to phenomenal transparency .
Two problems of fitting grief
Authors: Schönherr Julius
Published: August 2020

Recent years have seen a surge in philosophical work on the rationality of grief. Much of this research is premised on the idea that people tend to grieve much less than would be appropriate or, as it is often called, fitting. My goal in this paper is diagnostic, that is, to articulate two never properly distinguished, and indeed often conflated, arguments in favour of the purported discrepancy between experienced and fitting grief: a metaphysical and a psychological argument. According to the former, grief is rationalized entirely by facts about the past. And because the past is unchangeable, grief can be said to remain forever fitting. According to the latter argument, humans’ emotional resilience causes grief to diminish at a faster rate than would be fitting. Which of these problems we end up facing depends on relatively subtle variations in the characterization of the losses that render grief appropriate .
Offspring beg more toward larger females in a burying beetle
Authors: Ratz Tom , Stenson Sophie , Smiseth Per T
Published: August 2020

Offspring of many animals beg for food from parents. Begging is often costly, and offspring should seek to reduce such costs to maximize their returns on begging. Whenever multiple adults provide care for a joint brood, as in species where multiple females breed communally, offspring should beg toward the parent that provisions the most food. Here, we investigate whether larvae spend more time begging toward larger females in the burying beetle Nicrophorus vespilloides. Prior work on this species shows that larger females provision more food than smaller ones, suggesting that larvae would benefit by preferentially begging toward larger females. To test for such a preference, we provided experimental broods with a simultaneous choice between two dead females: a smaller and a larger one. Larvae spent more time begging toward larger females. We next examined the behavioral mechanism for why larvae begged more toward larger females. Larvae spent more time in close contact with larger females over smaller ones, whereas there was no evidence that larvae begged more when in close contact with larger females. Thus, larvae begged more toward the larger female simply as a consequence of spending more time close to larger females. Our findings have important implications for our understanding of parent–offspring communication by showing that offspring can choose between parents based on parental attributes, such as body size, reflecting how much food parents are likely to provision.
Foraging behavior and extended phenotype independently affect foraging success in spiders
Authors: DiRienzo N , Schraft H A , Montiglio P O , Bradley C T , Dornhaus A
Published: August 2020

Multiple phenotypic traits often interact with each other to determine an individual’s fitness. Behavioral and extended phenotypic traits, such as architectural constructions, can contribute to fitness in an integrated way. The goal of this study was to understand how the interaction between behavioral and extended phenotypic traits can affect foraging success. We tested this question using black widow spiders, where spiders that are aggressive in a foraging context tend to build more gumfooted silk lines that aid in prey capture, while non-aggressive spiders build webs with fewer gumfooted lines. We repeatedly assessed behavior and web structure to quantify relationships between these traits, and then allowed spiders to forage for live prey on their own web or the web of a conspecific that differed in structure. Thus, we assessed how varying combinations of behavior and web structure affect foraging success, and if correlational selection might act on them. We confirmed that aggressiveness and number of gumfooted lines are positively correlated and found that capture success increased with both aggressiveness and the number of gumfooted lines. Yet, we did not find any evidence for correlational selection: aggressiveness and number of gumfooted lines appeared to affect foraging success independently of each other. These findings highlight that a correlation between traits that contribute towards the same ecological function does not necessarily imply correlational selection. Taking advantage of the experimental convenience afforded by extended phenotypic traits can provide insight into the functional consequences of phenotypic variation within and between individuals.
LncAS2Cancer: a comprehensive database for alternative splicing of lncRNAs across human cancers
Authors: Deng Yulan , Luo Hao , Yang Zhenyu , Liu Lunxu
Published: August 2020

Accumulating studies demonstrated that the roles of lncRNAs for tumorigenesis were isoform-dependent and their aberrant splicing patterns in cancers contributed to function specificity. However, there is no existing database focusing on cancer-related alternative splicing of lncRNAs. Here, we developed a comprehensive database called LncAS2Cancer, which collected 5335 bulk RNA sequencing and 1826 single-cell RNA sequencing samples, covering over 30 cancer types. By applying six state-of-the-art splicing algorithms, 50 859 alternative splicing events for 8 splicing types were identified and deposited in the database. In addition, the database contained the following information: (i) splicing patterns of lncRNAs under seven different conditions, such as gene interference, which facilitated to infer potential regulators; (ii) annotation information derived from eight sources and manual curation, to understand the functional impact of affected sequences; (iii) survival analysis to explore potential biomarkers; as well as (iv) a suite of tools to browse, search, visualize and download interesting information. LncAS2Cancer could not only confirm the known cancer-associated lncRNA isoforms but also indicate novel ones. Using the data deposited in LncAS2Cancer, we compared gene model and transcript overlap between lncRNAs and protein-coding genes and discusses how these factors, along with sequencing depth, affected the interpretation of splicing signals. Based on recurrent signals and potential confounders, we proposed a reliable score to prioritize splicing events for further elucidation. Together, with the broad collection of lncRNA splicing patterns and annotation, LncAS2Cancer will provide important new insights into the diverse functional roles of lncRNA isoforms in human cancers. LncAS2Cancer is freely available at https://lncrna2as.cd120.com/.
A comprehensive survey of regulatory network inference methods using single cell RNA sequencing data
Authors: Nguyen Hung , Tran Duc , Tran Bang , Pehlivan Bahadir , Nguyen Tin
Published: August 2020

Gene regulatory network is a complicated set of interactions between genetic materials, which dictates how cells develop in living organisms and react to their surrounding environment. Robust comprehension of these interactions would help explain how cells function as well as predict their reactions to external factors. This knowledge can benefit both developmental biology and clinical research such as drug development or epidemiology research. Recently, the rapid advance of single-cell sequencing technologies, which pushed the limit of transcriptomic profiling to the individual cell level, opens up an entirely new area for regulatory network research. To exploit this new abundant source of data and take advantage of data in single-cell resolution, a number of computational methods have been proposed to uncover the interactions hidden by the averaging process in standard bulk sequencing. In this article, we review 15 such network inference methods developed for single-cell data. We discuss their underlying assumptions, inference techniques, usability, and pros and cons. In an extensive analysis using simulation, we also assess the methods’ performance, sensitivity to dropout and time complexity. The main objective of this survey is to assist not only life scientists in selecting suitable methods for their data and analysis purposes but also computational scientists in developing new methods by highlighting outstanding challenges in the field that remain to be addressed in the future development.
ProtFold-DFG: protein fold recognition by combining Directed Fusion Graph and PageRank algorithm
Authors: Shao Jiangyi , Liu Bin
Published: August 2020

As one of the most important tasks in protein structure prediction, protein fold recognition has attracted more and more attention. In this regard, some computational predictors have been proposed with the development of machine learning and artificial intelligence techniques. However, these existing computational methods are still suffering from some disadvantages. In this regard, we propose a new network-based predictor called ProtFold-DFG for protein fold recognition. We propose the Directed Fusion Graph (DFG) to fuse the ranking lists generated by different methods, which employs the transitive closure to incorporate more relationships among proteins and uses the KL divergence to calculate the relationship between two proteins so as to improve its generalization ability. Finally, the PageRank algorithm is performed on the DFG to accurately recognize the protein folds by considering the global interactions among proteins in the DFG. Tested on a widely used and rigorous benchmark data set, LINDAHL dataset, experimental results show that the ProtFold-DFG outperforms the other 35 competing methods, indicating that ProtFold-DFG will be a useful method for protein fold recognition. The source code and data of ProtFold-DFG can be downloaded from http://bliulab.net/ProtFold-DFG/download
Recognition of Parental Love: Birth Parents’ Experiences with Cooperation When Having a Child Placed in Family Foster Care in Denmark
Authors: Bengtsson Tea Torbenfeldt , Karmsteen Kirstine
Published: August 2020

Despite the fact that most children growing up in family foster care remain in contact with their birth parents, the experiences of birth parents have been largely neglected. This article draws on twenty-two in-depth interviews with birth parents to examine their experiences of cooperating with social workers and foster carers. Five patterns of cooperation are empirically identified in the parents’ narratives: (i) constructive cooperation; (ii) ambivalent cooperation; (iii) improved cooperation; (iv) diminishing cooperation and (v) lack of cooperation. While there are key differences in the characteristics of each pattern, there is one central similarity: all the parents seek recognition of their parenthood, especially their love for their child. Thus, actively recognising the role of parental love in parents’ motivation for cooperation can enable social workers to secure better cooperation with birth parents.
‘This is Still All about Love’: Practitioners’ Perspectives of Working with Family Carers Affected by the Harmful Behaviour of the Older Person for Whom They Care
Authors: Isham Louise , Bradbury-Jones Caroline , Hewison Alistair
Published: August 2020

This article explores a hidden and under-acknowledged dimension of caring in family life: when older people with care needs act in a harmful, abusive or violent way towards the family member(s) who cares for them. Thirty-eight health and social care professionals, working in the UK, took part in five focus groups to explore their experience of working with families in this situation. The group discussions were stimulated by vignettes developed from interviews with carers affected by harmful behaviour, and the data generated using this method were analysed using a thematic approach. There were two principal findings: (i) ‘carer harm’ is a serious and under-acknowledged problem, which practitioners have extensive experiential knowledge of and (ii) practitioners face considerable practical and ethical challenges working with affected families. Drawing on Miranda Fricker’s concept of epistemic injustice, we discuss how contemporary social, legal and policy systems can make it difficult for practitioners to identify and meet the needs of affected families. Finally, we explore the role of social workers—and the support they need as a professional group—to work alongside a ‘hidden’ group of families seeking to manage the intersection of care, harm and intimacy in later life.
PET imaging of meningioma with 18F-FLT: a predictor of tumour progression
Authors: Bashir Asma , Vestergaard Mark B , Marner Lisbeth , Larsen Vibeke A , Ziebell Morten , Fugleholm Kåre , Law Ian
Published: August 2020

We have previously reported that PET with 3′-deoxy-3′-18F-fluorothymidine (18F-FLT) provides a non-invasive assessment of cell proliferation in vivo in meningiomas. The purpose of this prospective study was to evaluate the potential of 18F-FLT PET in predicting subsequent tumour progression in asymptomatic meningiomas. Forty-three adult patients harbouring 46 MRI-presumed (n = 40) and residual meningiomas from previous surgery (n = 6) underwent a 60-min dynamic 18F-FLT PET scan prior to radiological surveillance. Maximum and mean tumour-to-blood ratios (TBRmax, TBRmean) of tracer radioactivity were calculated. Tumour progression was defined according to the latest published trial end-point criteria for bidimensional (2D) and corresponding yet exploratory volumetric measurements from the Response Assessment of Neuro-Oncology (RANO) workgroup. Independent-sample t-test, Pearson correlation coefficient, Cox regression, and receiver operating characteristic (ROC) curve analyses were used whenever appropriate. The median follow-up time after 18F-FLT PET imaging was 18 months (range 5–33.5 months). A high concordance rate (91%) was found with regard to disease progression using 2D-RANO (n = 11) versus volumetric criteria (n = 10). Using 2D-RANO criteria, 18F-FLT uptake was significantly increased in patients with progressive disease, compared to patients with stable disease (TBRmax, 5.5 ± 1.3 versus 3.6 ± 1.1, P < 0.0001; TBRmean, 3.5 ± 0.8 versus 2.4 ± 0.7, P < 0.0001). ROC analysis yielded optimal thresholds of 4.4 for TBRmax [sensitivity 82%, specificity 77%, accuracy 78%, and area under curve (AUC) 0.871; P < 0.0001] and 2.8 for TBRmean (sensitivity 82%, specificity 77%, accuracy 78%, AUC 0.848; P = 0.001) for early differentiation of patients with progressive disease from patients with stable disease. Upon excluding patients with residual meningioma or patients with stable disease with less than 12 months follow-up, the thresholds remained unchanged with similar diagnostic accuracies. Moreover, positive correlations were found between absolute and relative tumour growth rates and 18F-FLT uptake (r < 0.513, P < 0.015) that remained similar when excluding patients with residual meningioma or patients with stable disease and shorter follow-up period. Diagnostic accuracies were slightly inferior at 76% when assessing disease progression using volumetric criteria, while the thresholds remained unchanged. Multivariate analysis revealed that TBRmax was the only independent predictor of tumour progression (P < 0.046), while age, gender, baseline tumour size, tumour location, peritumoural oedema, and residual meningioma had no influence. The study reveals that 18F-FLT PET is a promising surrogate imaging biomarker for predicting subsequent tumour progression in treatment-naïve and asymptomatic residual meningiomas.
Splicing machinery dysregulation drives glioblastoma development/aggressiveness: oncogenic role of SRSF3
Authors: Fuentes-Fayos Antonio C , Vázquez-Borrego Mari C , Jiménez-Vacas Juan M , Bejarano Leire , Pedraza-Arévalo Sergio , L.-López Fernando , Blanco-Acevedo Cristóbal , Sánchez-Sánchez Rafael , Reyes Oscar , Ventura Sebastián , Solivera Juan , Breunig Joshua J , Blasco María A , Gahete Manuel D , Castaño Justo P , Luque Raúl M
Published: August 2020

Glioblastomas remain the deadliest brain tumour, with a dismal ∼12–16-month survival from diagnosis. Therefore, identification of new diagnostic, prognostic and therapeutic tools to tackle glioblastomas is urgently needed. Emerging evidence indicates that the cellular machinery controlling the splicing process (spliceosome) is altered in tumours, leading to oncogenic splicing events associated with tumour progression and aggressiveness. Here, we identify for the first time a profound dysregulation in the expression of relevant spliceosome components and splicing factors (at mRNA and protein levels) in well characterized cohorts of human high-grade astrocytomas, mostly glioblastomas, compared to healthy brain control samples, being SRSF3, RBM22, PTBP1 and RBM3 able to perfectly discriminate between tumours and control samples, and between proneural-like or mesenchymal-like tumours versus control samples from different mouse models with gliomas. Results were confirmed in four additional and independent human cohorts. Silencing of SRSF3, RBM22, PTBP1 and RBM3 decreased aggressiveness parameters in vitro (e.g. proliferation, migration, tumorsphere-formation, etc.) and induced apoptosis, especially SRSF3. Remarkably, SRSF3 was correlated with patient survival and relevant tumour markers, and its silencing in vivo drastically decreased tumour development and progression, likely through a molecular/cellular mechanism involving PDGFRB and associated oncogenic signalling pathways (PI3K-AKT/ERK), which may also involve the distinct alteration of alternative splicing events of specific transcription factors controlling PDGFRB (i.e. TP73). Altogether, our results demonstrate a drastic splicing machinery-associated molecular dysregulation in glioblastomas, which could potentially be considered as a source of novel diagnostic and prognostic biomarkers as well as therapeutic targets for glioblastomas. Remarkably, SRSF3 is directly associated with glioblastoma development, progression, aggressiveness and patient survival and represents a novel potential therapeutic target to tackle this devastating pathology.
Lipid Pathology of the Corpus Callosum in Schizophrenia and the Potential Role of Abnormal Gene Regulatory Networks with Reduced Microglial Marker Expression
Authors: Shimamoto-Mitsuyama Chie , Nakaya Akihiro , Esaki Kayoko , Balan Shabeesh , Iwayama Yoshimi , Ohnishi Tetsuo , Maekawa Motoko , Toyota Tomoko , Dean Brian , Yoshikawa Takeo
Published: August 2020

Structural changes in the corpus callosum have been reported in schizophrenia; however, the underlying molecular mechanism remains unclear. As the corpus callosum is high in lipid content, we analyzed the lipid contents of the corpora callosa from 15 patients with schizophrenia and 15 age- and sex-matched controls using liquid chromatography coupled to tandem mass spectrometry and identified lipid combinations associated with schizophrenia. Real-time quantitative polymerase chain reaction analyses using extended samples (schizophrenia, n = 95; control, n = 91) showed low expression levels of lipid metabolism-related genes and their potential upstream transcription factors in schizophrenia. Subsequent pathway analysis identified a gene regulatory network where nuclear factor of activated T cells 2 (NFATC2) is placed most upstream. We also observed low gene expression levels of microglial markers, inflammatory cytokines, and colony-stimulating factor 1 receptor (CSF1R), which is known to regulate the density of microglia, in the corpus callosum in schizophrenia. The interactions between CSF1R and several genes in the presently identified gene network originating from NFATC2 have been reported. Collectively, this study provides evidence regarding lipid abnormalities in the corpora callosa of patients with schizophrenia and proposes the potential role of impaired “NFATC2-relevant gene network-microglial axis” as its underlying mechanism.
An Effective Acid–Base-Induced Liquid–Liquid Microextraction Based on Deep Eutectic Solvents for Determination of Testosterone and Methyltestosterone in Milk
Authors: Li Xiao , Yuan Tao , Zhao Ting , Wu Xiaomei , Yang Yaling
Published: August 2020

An environmentally friendly method for the determination of testosterone and methyltestosterone by acid–base-induced deep eutectic solvents liquid–liquid microextraction (DES-ABLLME) combining with high-performance liquid chromatography was established. The deep eutectic solvent (DES) consisting of menthol:lauric acid:decanoic acid (3:1:1) can act as both hydrogen bond donor and hydrogen bond acceptor. In this approach, ammonia solution (NH3•H2O) is used as an emulsifier to react with DESs in the extraction process to generate salt and form milky white solution, achieving high extraction efficiency. Hydrochloric acid was used as a phase separator to change the emulsification state and promote the separation of extraction agent from water phase. A series of parameters were optimized including the volume of DES and the emulsifying agent, glucose concentration as well as hydrochloric acid volume. The method was linear in the range 0.5–100 μg mL−1 with a correlation coefficient (R) of 0.9999, and the limits of detection were 0.067 and 0.2 μg mL−1 for testosterone and methyltestosterone, respectively. This method was applied to analyze testosterone and methyltestosterone in milk samples, and the recoveries were between 89.2 and 108.2%.
Adverse Events Among Young Adults Following a Third Dose of Measles-Mumps-Rubella Vaccine
Authors: Marin Mona , Fiebelkorn Amy Parker , Bi Daoling , Coleman Laura A , Routh Janell , Curns Aaron T , McLean Huong Q
Published: August 2020

Background

A third measles-mumps-rubella vaccine (MMR) dose (MMR3) is recommended in the United States for persons at increased risk for mumps during outbreaks. MMR3 is also likely given to persons who might have received 2 doses of MMR but lack documentation. Since MMR3 safety data are limited, we describe adverse events in persons receiving MMR3 in a nonoutbreak setting.

Methods

Young adults with 2 documented MMR doses were administered MMR3. From 2 weeks before until 4 weeks after MMR3 receipt, participants reported daily on 11 solicited, common symptoms potentially associated with MMR. Weekly rate differences in post- vs prevaccination (baseline) were evaluated by Poisson regression. Baseline rates were subtracted from postvaccination rates of significantly different symptoms to estimate the number and percentage of participants with excess risk for symptoms post-MMR3. Descriptive analyses were performed for 3 postvaccination injection-site symptoms.

Results

The 662 participants were aged 18–28 years (median = 20 years); 56% were women. Headache, joint problems, diarrhea, and lymphadenopathy rates were significantly higher postvaccination vs baseline. We estimate that 119 participants (18%) reported more symptoms after MMR3 than prevaccination. By symptom, 13%, 10%, 8%, and 6% experienced increased symptoms of headache, joint problems, diarrhea, and lymphadenopathy, respectively, after MMR3. The median onset was Days 3–6 postvaccination; the median duration was 1–2 days. One healthcare visit for a potential vaccination-related symptom (urticaria) was reported. Injection-site symptoms were reported by 163 participants (25%); the median duration was 1–2 days.

Conclusions

Reported systemic and local events were mild and transient. MMR3 is safe and tolerable among young adults.
A Pediatric Infectious Disease Perspective on COVID-19
Authors: Wald Ellen R , Schmit Kathryn M , Gusland Daniele Y
Published: August 2020

This review highlights the clinical and epidemiologic characteristics of coronavirus disease 2019 (COVID-19) in children and neonates and contrasts these features with other common respiratory viruses. Although the majority of infections in children are mild, there are many important, as yet, unanswered questions (specifically, the attack rate in children and the role of children as vectors of infection) that will have a major impact on disease in adults. There are no distinctive clinical characteristics that will allow the infectious disease consultant to make the diagnosis without laboratory testing. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) appears to be less common, with lower morbidity and mortality than respiratory syncytial virus or influenza, and causes less-severe disease in children with cancer than these more common viruses. The range of severity of infection during pregnancy is comparable to infection in nonpregnant cohorts. Intrauterine infection has been documented but is uncommon. A theme of less-severe disease in individuals with modulated immune systems is emerging.
Use of a Latent Class Analysis in the Diagnosis of Chronic Chagas Disease in the Washington Metropolitan Area
Authors: Castro-Sesquen Yagahira E , Saldaña Antonella , Patino Nava Dhayanna , Bayangos Tabitha , Paulette Evans Diana , DeToy Kelly , Trevino Alexia , Marcus Rachel , Bern Caryn , Gilman Robert H , Talaat Kawsar R ,   Chagas Working Group in Peru and the United States, Avila Carol , Camacho Fabiola , Herrera Sdenka , Jimenez Andres , Lozano Veronika , Malaga Edith , Merida Mariel , Morales Carolina , Solis Rodrigo , Sotomayor Fiorella , Tung Alisha , Spector Anna , Verastegui Manuela , Yang Younghee , Zapata Fatima
Published: August 2020

Background

The diversity of individuals at risk for Trypanosoma cruzi infection in the United States poses challenges for diagnosis. We evaluated the diagnostic accuracy of Food and Drug Administration (FDA)-cleared tests in the Washington Metropolitan area (WMA).

Methods

In total, 1514 individuals were evaluated (1078 from Mexico, Central and northern South America [TcI-predominant areas], and 436 from southern South America [TcII/V/VI-predominant areas]). Optical density (OD) values from the Hemagen EIA and Chagatest v.3 Wiener, and categorical results of the IgG-TESA-blot (Western blot with trypomastigote excretory-secretory antigen), and the Chagas detect plus (CDP), as well as information of area of origin were used to determine T. cruzi serostatus using latent class analysis.

Results

We detected 2 latent class (LC) of seropositives with low (LC1) and high (LC2) antibody levels. A significantly lower number of seropositives were detected by the Wiener, IgG-TESA-blot, and CDP in LC1 (60.6%, P < .001, 93.1%, P = .014, and 84.9%, P = .002, respectively) as compared to LC2 (100%, 100%, and 98.2%, respectively). LC1 was the main type of seropositives in TcI-predominant areas, representing 65.0% of all seropositives as opposed to 22.8% in TcII/V/VI-predominant areas. The highest sensitivity was observed for the Hemagen (100%, 95% confidence interval [CI]: 96.2–100.0), but this test has a low specificity (90.4%, 95% CI: 88.7–91.9). The best balance between positive (90.9%, 95% CI: 83.5–95.1), and negative (99.9%, 95% CI: 99.4–99.9) predictive values was obtained with the Wiener.

Conclusions

Deficiencies in current FDA-cleared assays were observed. Low antibody levels are the main type of seropositives in individuals from TcI-predominant areas, the most frequent immigrant group in the United States.
Clinical Outcomes Associated With Methylprednisolone in Mechanically Ventilated Patients With COVID-19
Authors: Nelson Brian C , Laracy Justin , Shoucri Sherif , Dietz Donald , Zucker Jason , Patel Nina , Sobieszczyk Magdalena E , Kubin Christine J , Gomez-Simmonds Angela
Published: August 2020

Background

The efficacy and safety of methylprednisolone in mechanically ventilated patients with acute respiratory distress syndrome resulting from coronavirus disease 2019 (COVID-19) are unclear. In this study, we evaluated the association between use of methylprednisolone and key clinical outcomes.

Methods

Clinical outcomes associated with the use of methylprednisolone were assessed in an unmatched, case-control study; a subset of patients also underwent propensity-score matching. Patients were admitted between 1 March and 12 April, 2020. The primary outcome was ventilator-free days by 28 days after admission. Secondary outcomes included extubation, mortality, discharge, positive cultures, and hyperglycemia.

Results

A total of 117 patients met inclusion criteria. Propensity matching yielded a cohort of 42 well-matched pairs. Groups were similar except for hydroxychloroquine and azithromycin use, which were more common in patients who did not receive methylprednisolone. Mean ventilator-free days were significantly higher in patients treated with methylprednisolone (6.21 ± 7.45 vs 3.14 ± 6.22; P = .044). The probability of extubation was also increased in patients receiving methylprednisolone (45% vs 21%; P = .021), and there were no significant differences in mortality (19% vs 36%; P = .087). In a multivariable linear regression analysis, only methylprednisolone use was associated with a higher number of ventilator-free days (P = .045). The incidence of positive cultures and hyperglycemia were similar between groups.

Conclusions

Methylprednisolone was associated with increased ventilator-free days and higher probability of extubation in a propensity-score matched cohort. Randomized, controlled studies are needed to further define methylprednisolone use in patients with COVID-19.
Effects of a Water, Sanitation, and Hygiene Mobile Health Program on Diarrhea and Child Growth in Bangladesh: A Cluster-randomized Controlled Trial of the Cholera Hospital-based Intervention for 7 Days (CHoBI7) Mobile Health Program
Authors: George Christine Marie , Monira Shirajum , Zohura Fatema , Thomas Elizabeth D , Hasan M Tasdik , Parvin Tahmina , Hasan Khaled , Rashid Mahamud-ur , Papri Nowshin , Islam Aminul , Rahman Zillur , Rafique Raisa , Islam Bhuyian Md Sazzadul , Saxton Ronald , Labrique Alain , Alland Kelsey , Barman Indrajeet , Jubyda Fatema Tuz , Afroze Farzana , Sultana Marzia , Johura Fatema-Tuz , Khan Md Abul Hasem , Tahmina Sanya , Munmun Farzana , Sack David A , Perin Jamie , Alam Munirul
Published: August 2020

Background

The Cholera Hospital-Based Intervention for 7 Days (CHoBI7) mobile health (mHealth) program was a cluster-randomized controlled trial of diarrhea patient households conducted in Dhaka, Bangladesh.

Methods

Patients were block-randomized to 3 arms: standard message on oral rehydration solution use; health facility delivery of CHoBI7 plus mHealth (no home visits); and health facility delivery of CHoBI7 plus 2 home visits and mHealth. The primary outcome was reported diarrhea in the past 2 weeks collected monthly for 12 months. The secondary outcomes were stunting, underweight, and wasting at a 12-month follow-up. Analysis was intention-to-treat.

Results

Between 4 December 2016 and 26 April 2018, 2626 participants in 769 households were randomly allocated to 3 arms: 849 participants to the standard message arm, 886 to mHealth with no home visits arm, and 891 to the mHealth with 2 home visits. Children <5 years had significantly lower 12-month diarrhea prevalence in both the mHealth with 2 home visits arm (prevalence ratio [PR]: 0.73 [95% confidence interval {CI}, .61–.87]) and the mHealth with no home visits arm (PR: 0.82 [95% CI, .69–.97]). Children <2 years were significantly less likely to be stunted in both the mHealth with 2 home visits arm (33% vs 45%; odds ratio [OR]: 0.55 [95% CI, .31–.97]) and the mHealth with no home visits arm (32% vs 45%; OR: 0.54 [95% CI, .31–.96]) compared with children in the standard message arm.

Conclusions

The CHoBI7 mHealth program lowered pediatric diarrhea and stunting among diarrhea patient households.

Clinical Trials Registration

NCT04008134.
The effect of phosphate binder therapy with sucroferric oxyhydroxide on calcification propensity in chronic haemodialysis patients: a randomized, controlled, crossover trial
Authors: Thiem Ursula , Soellradl Ina , Robl Bernhard , Watorek Ewa , Blum Sabine , Dumfarth Alexandra , Marculescu Rodrig , Pasch Andreas , Haller Maria C , Cejka Daniel
Published: August 2020

Background

Calcification propensity is associated with the risk for cardiovascular events and death in end-stage renal disease patients. Here we investigated the effect of lowering serum phosphate with oral phosphate binder therapy on calcification propensity.

Methods

We performed an open-label, randomized, controlled, crossover study in chronic haemodialysis patients with hyperphosphataemia. Patients (n = 39) were randomized in a 1:1 ratio to either low-dose (250 mg/day) sucroferric oxyhydroxide (SO) followed by high-dose (2000 mg/day) SO or vice versa, with washout phases before and after SO treatment. The primary endpoint was changed in calcification propensity as measured by calciprotein particle formation time (T50 test) between washout and high-dose SO treatment in patients with ≥85% adherence to the prescribed SO dose (per-protocol analysis).

Results

In the primary per-protocol analysis (n = 28), 2000 mg/day SO treatment resulted in a mean increase in T50 of 66 min (95% CI 49–84 min, P < 0.0001), from 243 ± 63 to 309 ± 74 min compared with phosphate binder washout. Serum phosphate decreased from 2.28 ± 0.5 to 1.63 ± 0.43 mmol/L (P < 0.0001). SO at 250 mg/day did not influence T50 (P = 0.4) or serum phosphate concentrations (P = 0.9) compared with phosphate binder washout. The secondary intention-to-treat analysis (n = 39) showed similar results: an increase in T50 of 52 min (95% CI 31–74 min, P < 0.0001) and a decrease in serum phosphate from 2.18 ± 0.5 to 1.64 ± 0.46 mmol/L. No major adverse cardiovascular event, case of calciphylaxis or death occurred during the study.

Conclusion

Phosphate binder treatment with SO improves serum calcification propensity of haemodialysis patients and might lead to improved outcomes.
Long-term consequences of acute kidney injury: a narrative review
Authors: Gameiro Joana , Marques Filipe , Lopes José António
Published: August 2020

The incidence of acute kidney injury (AKI) has increased in the past decades. AKI complicates up to 15% of hospitalizations and can reach up to 50–60% in critically ill patients. Besides the short-term impact of AKI in patient outcomes, several studies report the association between AKI and adverse long-term outcomes, such as recurrent AKI episodes in 25–30% of cases, hospital re-admissions in up to 40% of patients, an increased risk of cardiovascular events, an increased risk of progression of chronic kidney disease (CKD) after AKI and a significantly increased long-term mortality. Despite the long-term impact of AKI, there are neither established guidelines on the follow-up care of AKI patients, nor treatment strategies to reduce the incidence of sequelae after AKI. Only a minority of patients have been referred to nephrology post-discharge care, despite the evidence of improved outcomes associated with nephrology referral by addressing cardiovascular risk and risk of progression to CKD. Indeed, AKI survivors should have specialized nephrology follow-up to assess kidney function after AKI, perform medication reconciliation, educate patients on nephrotoxic avoidance and implement strategies to prevent CKD progression. The authors provide a comprehensive review of the transition from AKI to CKD, analyse the current evidence on the long-term outcomes of AKI and describe predisposing risk factors, highlight the importance of follow-up care in these patients and describe the current therapeutic strategies which are being investigated on their impact in improving patient outcomes.
Acute kidney injury may impede results after transcatheter aortic valve implantation
Authors: Haase-Fielitz Anja , Altendeitering Fiona , Iwers Ragna , Sliziuk Veronika , Barabasch Sophie , Bannehr Marwin , Hähnel Valentin , Neuss Michael , Haase Michael , Apfelbacher Christian , Butter Christian
Published: August 2020

Introduction

Severe complications after transcatheter aortic valve implantation (TAVI) are rare due to increasing procedural safety. However, TAVI procedure-related haemodynamic instability and increased risk of infection may affect renal functional reserve with subsequent renal acidosis and hyperkalaemia.

Objective

In this study, we investigated incidence, modifiable risk factors and prognosis of acute kidney injury (AKI) and AKI complicated by hyperkalaemia, pulmonary oedema or metabolic acidosis after TAVI.

Methods

In a retrospective single-centre study, 804 consecutive patients hospitalized during 2017 and 2018 for elective TAVI were included. AKI was defined according to the ‘Kidney Disease Improving Global Outcome’ (KDIGO) initiative. Variables on co-morbidities, intra-/post-interventional complications and course of renal function up to 6 months after index-hospitalization were assessed. In multivariate regression analyses, risk factors for the development of AKI, complicated AKI, renal non-recovery from AKI and in-hospital mortality were determined.

Results

Incidence of AKI was 13.8% (111/804); in-hospital mortality after TAVI was 2.3%. AKI was an independent risk factor for in-hospital mortality, odds ratio (OR) 10.3 (3.4–31.6), P < 0.001, further increasing to OR = 21.8 (6.6–71.5), P < 0.001 in patients with AKI complicated by hyperkalaemia, pulmonary oedema or metabolic acidosis, n = 57/111 (51.4%). Potentially modifiable, interventional factors independently associated with complicated AKI were infection [OR = 3.20 (1.61–6.33), P = 0.001] and red blood cell transfusion [OR = 5.04 (2.67–9.52), P < 0.001]. Valve type and size, contrast volume and other intra-interventional characteristics, such as the need for tachycardial pacing, did not influence the development of AKI. Eleven of 111 (9.9%) patients did not recover from AKI, mostly affecting patients with cardiac decompensation. In 18/111 (16.2%) patients, information concerning AKI was provided in discharge letter. Within 6 months after TAVI, higher proportion of patients with AKI showed progression of pre-existing chronic kidney disease compared with patients without AKI [14/29, 48.3% versus 54/187, 28.9%, OR = 2.3 (95% confidence interval 1.0–5.1), P = 0.036].

Conclusions

AKI is common and may impede patient outcome after TAVI with acute complications such as hyperkalaemia or metabolic acidosis and adverse renal function until 6 months after intervention. Our study findings may contribute to refinement of allocation of appropriate level of care in and out of hospital after TAVI.
Lost In Translation: Exposing Hidden Compiler Optimization Opportunities
Authors: Georgiou Kyriakos , Chamski Zbigniew , Amaya Garcia Andres , May David , Eder Kerstin
Published: August 2020

Existing iterative compilation and machine learning-based optimization techniques have been proven very successful in achieving better optimizations than the standard optimization levels of a compiler. However, they were not engineered to support the tuning of a compiler’s optimizer as part of the compiler’s daily development cycle. In this paper, we first establish the required properties that a technique must exhibit to enable such tuning. We then introduce an enhancement to the classic nightly routine testing of compilers, which exhibits all the required properties and thus is capable of driving the improvement and tuning of the compiler’s common optimizer. This is achieved by leveraging resource usage and compilation information collected while systematically exploiting prefixes of the transformations applied at standard optimization levels. Experimental evaluation using the LLVM v6.0.1 compiler demonstrated that the new approach was able to reveal hidden cross-architecture and architecture-dependent potential optimizations on two popular processors: the Intel i5-6300U and the Arm Cortex-A53-based Broadcom BCM2837 used in the Raspberry Pi 3B+. As a case study, we demonstrate how the insights from our approach enabled us to identify and remove a significant shortcoming of the CFG simplification pass of the LLVM v6.0.1 compiler.
Advancing scientific knowledge and conservation of birds through inclusion of conservation social sciences in the American Ornithological Society
Authors: Dayer Ashley A , Barnes Jessica C , Dietsch Alia M , Keating Jacqueline M , Naves Liliana C
Published: August 2020

Conservation efforts are shaped by individual and collective human behaviors, cultural norms and values, economic pressures, and political and organizational structures. As such, the conservation social sciences—disciplines that draw on social science theories and approaches to improve conservation efforts—can play a vital role in advancing the science and practice of bird conservation. We connect the rich, ongoing discussion about the vital role of the conservation social sciences to the specific context of bird conservation and make an argument for the importance of proactive inclusion of these sciences in ornithological societies. First, we introduce the conservation social sciences and illustrate how they can improve the design and implementation of conservation programs and policies for birds. Drawing on discussions from a symposium we organized at the 2019 American Ornithological Society (AOS) annual meeting, we encourage the AOS to make institutional changes that could further support the inclusion of conservation social sciences. These changes ideally would include a working group, conference plenaries and themes, and high-quality social science publications, along with support and encouragement for ornithologists and bird conservationists to partake in trainings and collaborate with social scientists. Strategies for how to do so effectively can be adapted from other conservation societies that have paved the way for disciplinary inclusivity.
Validation of a novel method to create temporal records of hormone concentrations from the claws of ringed and bearded seals
Authors: Karpovich Shawna A , Horstmann Larissa A , Polasek Lori K
Published: August 2020

Ringed (Pusa hispida) and bearded seals (Erignathus barbatus) inhabit vast and often remote areas in the Arctic, making it difficult to obtain long-term physiological information concerning health and reproduction. These seals are experiencing climate-driven changes in their habitat that could result in physiological stress. Chronic physiological stress can lead to immunosuppression, decreased reproduction and decreased growth. Recently, keratin has become a popular matrix to measure steroid hormones, such as stress-related cortisol and reproduction-related progesterone. We developed and validated methods to extract cortisol and progesterone from the claws of adult female ringed (n = 20) and bearded (n = 3) seals using enzyme immunosorbent assays. As ringed and bearded seal claws grow, a pair of dark- and light-colored bands of keratin is deposited annually providing a guide for sampling. Two processing methods were evaluated, removal of claw material with a grinding bit or grinding followed by mechanical pulverization (102 paired samples from six claws, two each from three seals). Adding the mechanical pulverization step resulted in a 1.5-fold increase in hormone extraction. Progesterone from the proximal claw band was evaluated to biologically validate claw material as a measure of pregnancy in ringed seals (n = 14). Claws from pregnant seals had significantly higher claw progesterone concentrations than from non-pregnant seals. This suggests that the elevated progesterone associated with gestation was reflected in the claws, and that the most proximal claw band was indicative of pregnancy status at time of death. Thus, although the sample size was low and the collection dates unbalanced, this study demonstrates the potential to use claws to examine an extended time series (up to 12 yrs) of cortisol and progesterone concentrations in ringed and bearded seal claws.
Thoreau’s luminous Homer in A Week on the Concord and Merrimack Rivers
Authors: Parker Luke
Published: August 2020

Henry David Thoreau’s relationship to Greek literature, and Homer’s Iliad in particular, is more often remarked than analysed. This article argues that Thoreau’s engagement with Homer in his first book, A Week on the Concord and Merrimack Rivers, proves central to the themes of that work highlighted by critics as well as its less-studied formal hybrid of poetry and prose. I show that Thoreau constructs Homer as the poetic ideal in which the perennially renewed life of the natural world becomes accessible to human beings caught in the fatal and unidirectional movement of historical time. Thoreau’s ideas here may track Romantic conceptions of Homer and Greek literature more generally, but Thoreau turns contemporary uncertainty around the person of Homer into reflection on the relationship between personal experience and literary expression of ‘living nature’. This turns out to structure a larger dichotomy between poetry and prose, one in which Thoreau associates the latter with authentic experience and self-expression of an individual human life. In A Week’s engagement with Homer, then, we see Thoreau negotiating not only some core concerns of his writing but also his evolution from aspiring poet to author of the works in prose that ultimately define his career.