bluuebunny/crossref_metadata_2025 huggingface dataset:
Consistent Effects of Whey Protein Fortification on Consumer Perception and Liking of Solid Food Matrices (Cakes and Biscuits) Regardless of Age and Saliva Flow
Authors: Norton Victoria , Lignou Stella , Bull Stephanie P. , Gosney Margot A. , Methven Lisa
Published: September 2020

Although there are numerous high protein products on the market, they are typically not designed with, or for, older consumers. This is surprising considering that dietary guidelines recognise the need for higher protein intake in later life. Protein fortified products are, however, associated with negative sensory attributes and poor consumer acceptance. This paper investigates the extent of mouthdrying sensations within a high protein solid food matrix, along with the effect of age and saliva flow. Solid models using cakes and biscuits, with or without protein fortification, were investigated. The sensory profile and physical properties were analysed and two volunteer studies (n = 84; n = 70) were carried out using two age groups (18–30; 65+). Volunteers rated individual perception and liking of products, and salivary flow rates (mL/min) were measured. Unstimulated salivary flow rates were significantly lower (p < 0.05) in older adults, although this was not found to influence product perception. Protein fortification of cakes and biscuits significantly increased (p < 0.05) perceived mouthdrying, hardness and “off” flavours, and significantly reduced (p < 0.05) melting rate, moistness and liking compared with the control versions. There is a clear need to address negative sensory attributes associated with protein fortification of cakes and biscuits to ensure product suitability for older adults.
Modified Atmosphere and Humidity Film Reduces Browning Susceptibility of Oriental Melon Suture Tissue during Cold Storage
Authors: Park Me-Hea , Chang Eun-Ha , Yang Hae-Jo , Lee Jung-Soo , Do Gyung-Ran , Song Hyun Jong , Chang Min-Sun , Ku Kang-Mo
Published: September 2020

Oriental melons have a relatively short shelf life as they are harvested during the summer season and susceptible to cold-induced injuries. Typical chilling injury when stored at 4 °C is expressed as browning of the fruit suture. To prolong the shelf life and reduce browning of the fruit, the effects of modified atmosphere packaging (MAP), X-tend modified atmosphere (MA)/modified humidity (MH) bulk packaging (XF), and polyethylene (PE) packaging, on oriental melons were investigated during storage at 4 °C and 10 °C for 14 days and under retail display conditions at 20 °C. The O2 concentrations in PE packages stored at 4 °C and 10 °C ranged from 17.4 to 18.5%, whereas those in XF packages were reduced to 16.3–16.6%. The CO2 content of XF package (4.2–4.6%) was higher than that of PE package (1.4–1.9%) stored at 4 °C or 10 °C. Relative humidity (RH) saturated in the PE packages but not in the XF packages after seven days of storage. Furthermore, PE packages performed better at maintaining melon weight and firmness than XF packages during storage at 10 °C for 14 days and under retail display conditions at 20 °C. PE and XF packages effectively reduced the browning index of the peel and white linear sutures of oriental melons compared with the unpackaged control during cold storage at 4 °C, and this observation was maintained at the retail display condition at 20 °C. The enhanced CO2 levels, reduced O2 levels, and optimal RH values that were provided by the MAP, prevented the browning symptoms, and improved the marketability and shelf life of oriental melons.
Sensory and Volatile Profiles of Korean Commercially Distilled Soju Using Descriptive Analysis and HS-SPME-GC-MS
Authors: Hong Jung-Min , Kim Tae-Wan , Lee Seung-Joo
Published: September 2020

Volatile compositions and sensory characteristics of 11 commercially distilled soju samples were investigated using headspace solid-phase microextraction (HS-SPME) with gas chromatography-mass spectrometry (GC-MS) and sensory descriptive analysis. A total of 59 major volatile compounds, consisting of 32 esters, 10 alcohols, 2 acids, 5 aldehydes, 3 ketones, 1 hydrocarbon, 1 furan, 2 phenols, and 3 miscellaneous compounds, were identified. From the principal component analysis (PCA) of volatile data, MSJ made by atmospheric distillation showed a clear distinction in volatile compositions compared to that of other samples made by vacuum distillation. Based on PCA of the sensory data determined by a panel of ten judges, MSJ was associated with a large amount of longer chain esters that showed high intensities in bitter taste and yeast/nuruk-related flavor attributes. HYJ, LPJ, and HAJ made with rice as a raw material were associated with lower intensities of the alcohol aroma, while JRJ and OKJ aged in oak barrels were associated with fruit flavor, sweet flavor, and brandy aroma. In the partial least squares regression (PLSR) analysis to see any relationship between volatile and sensory data, longer chain esters like ethyl tetradecanoate, and ethyl hexadecanoate were highly associated with bleach aroma. In contrast, positive correlations were seen with barley aroma and yeast flavor with hexanal, nonanal, benzaldehyde, and 2-methoxy-phenol.
Microencapsulation of Horseradish (Armoracia rusticana L.) Juice Using Spray-Drying
Authors: Tomsone Lolita , Galoburda Ruta , Kruma Zanda , Durrieu Vanessa , Cinkmanis Ingmars
Published: September 2020

Horseradish contains many bioactive compounds with antioxidant activity. The current study aimed to evaluate the effect of various wall materials and their ratios on the physical properties and bioactive-compound retention and stability in microencapsulated horseradish leaf and root juices. Horseradish juice was microencapsulated using maltodextrin, maltodextrin/gum Arabic, soy protein isolate, and starch with three different core-to-wall ratios. The total phenolic, total flavonoid, total flavan-3-ol, and total phenolic-acid contents, as well as antioxidant activity, were determined using spectrophotometric methods, whereas individual phenol profiles were determined by high-performance liquid chromatography (HPLC). Multivariate analysis of variance showed that plant material, wall material, and core-to-wall ratio had a significant effect on the bioactive-compound retention and antioxidant-activity preservation. Microcapsules produced from horseradish leaf juice had a significantly higher content of phenolic compounds and antioxidant activity compared to root-juice microcapsules. However, better retention was observed for microencapsulated horseradish root juice. Maltodextrin and maltodextrin/gum Arabic were the most effective wall materials for the retention of bioactive compounds, while they also had a smaller particle size and better solubility. The horseradish-juice microcapsules possess a high content of rutin. The highest stability of bioactive compounds after storage was found at a core-to-wall ratio of 20:80.
Effect of Prior Chilling Period and Alga-Extract Packaging on the Quality of a Canned Underutilised Fish Species
Authors: Aubourg Santiago P. , Trigo Marcos , Martínez Beatriz , Rodríguez Alicia
Published: September 2020

The effect of a prior chilling period and an alga extract packaging on the quality of a canned underutilised mackerel species (Scomber colias) was investigated. For this different chilling times (0, 4 and 9 days) were taken into account and three concentrations of aqueous extracts of the macroalga Fucus spiralis were tested in a brine-packaging medium. Chemical changes related to quality were analysed after 3 months of canned storage. A substantial increase (p < 0.05) in free fatty acid content was observed in canned fish by increasing the chilling time; however, alga extract presence in the packaging medium led to decreased mean values. Concerning lipid oxidation development, an increased chilling time led to higher values (p < 0.05) of thiobarbituric acid index and fluorescent compounds formation; remarkably, an increased presence of alga extract led to a higher (p < 0.05) peroxide retention and lower (p < 0.05) fluorescent compounds content. Average colour L* and a* values showed a decrease and an increase, respectively, with chilling time; however, such changes were minimised with the alga extract content in the packaging system. Trimethylamine content revealed a marked increase as a result of the sterilisation step, but no influence (p > 0.05) of the chilling time or the alga-packaging medium could be implied.
Unveiling Sex-Based Differences in the Effects of Alcohol Abuse: A Comprehensive Functional Meta-Analysis of Transcriptomic Studies
Authors: Casanova Ferrer Franc , Pascual María , Hidalgo Marta R. , Malmierca-Merlo Pablo , Guerri Consuelo , García-García Francisco
Published: September 2020

The abuse of alcohol, one of the most popular psychoactive substances, can cause several pathological and psychological consequences, including alcohol use disorder (AUD). An impaired ability to stop or control alcohol intake despite adverse health or social consequences characterize AUD. While AUDs predominantly occur in men, growing evidence suggests the existence of distinct cognitive and biological consequences of alcohol dependence in women. The molecular and physiological mechanisms participating in these differential effects remain unknown. Transcriptomic technology permits the detection of the biological mechanisms responsible for such sex-based differences, which supports the subsequent development of novel personalized therapeutics to treat AUD. We conducted a systematic review and meta-analysis of transcriptomics studies regarding alcohol dependence in humans with representation from both sexes. For each study, we processed and analyzed transcriptomic data to obtain a functional profile of pathways and biological functions and then integrated the resulting data by meta-analysis to characterize any sex-based transcriptomic differences associated with AUD. Global results of the transcriptomic analysis revealed the association of decreased tissue regeneration, embryo malformations, altered intracellular transport, and increased rate of RNA and protein replacement with female AUD patients. Meanwhile, our analysis indicated that increased inflammatory response and blood pressure and a reduction in DNA repair capabilities are associated with male AUD patients. In summary, our functional meta-analysis of transcriptomic studies provides evidence for differential biological mechanisms of AUD patients of differing sex.
Feasibility of Using Strength Measures, Including Peak Inspiratory Flow, for Routine Monitoring in Case Management Patients Aged 65 and over
Authors: Barnes Nicola , Walsh Bronagh , Samuel Dinesh
Published: September 2020

Peak inspiratory flow (PIF) is a portable, relatively new method for measuring respiratory function and indirect muscle strength; the feasibility of its routine clinical measurement is unknown. To investigate the acceptability, reliability and short-term stability of PIF, alongside the established measures of peak expiratory flow (PEF) and grip strength in community dwelling case management patients. Patients were tested in a sitting position, initially on two occasions, one week apart; seven patients having repeated measures taken on a further four occasions over a seven-week period. The best of three attempts for all measures were recorded. Reliability was tested using intra-class correlation coefficient (ICC), standard error of measurement (SEM), minimal detectable change (MDC) and Bland–Altman analysis. Eight patients aged 69–91 years (mean age 81.5 ± 7.7 years; 5 males) participated. For between-day reliability using the first two time points, one week apart the ICCs (3,1) were 0.97, 0.98 and 0.99 for PIF, PEF and grip strength respectively; using all five time points resulted in ICCs of 0.92, 0.99 and 0.99 respectively. Bland–Altman plots also illustrated a good level of agreement across days. Feedback on the acceptability of the measures was gathered from patients. PIF, PEF and grip strength showed excellent reliability and acceptability. Whilst excellent reliability was observed over the seven-week period, the occurrence of clinically significant symptoms and adverse events in the presence of unchanging PIF, PEF and grip strength, suggests that the measures may not be suitable to identify patients with multiple health conditions entering a period of acute decline.
Knowledge, Attitude and Implementation of Evidence-Based Practice among Physiotherapists Working in the Kingdom of Saudi Arabia: A Cross-Sectional Survey
Authors: Hasani Fatmah , MacDermid Joy C. , Tang Ada , Kho Michelle , Alghadir Ahmad H. , Anwer Shahnawaz
Published: September 2020

The current study aimed to investigate knowledge, attitudes, and implementation of evidence-based practice among physiotherapists working in Saudi Arabia. A sample of physiotherapists working in various outpatient settings in Saudi Arabia participated in this survey. Sixty-four therapists (30 females, 34 males) completed a 28-item survey questionnaire. Approximately half of the participants indicated that evidence-based practice was useful and important for clinical practice. About 60% of the participants reported that they have adopted the evidence-based practice on a regular basis. Participants who had a membership in a physiotherapy organization and had advanced degrees showed more positive attitudes (t = −2.31, p = 0.02 and t = −2.15, p = 0.04, respectively) and greater levels of knowledge (t = −2.32, p = 0.02 and t = −3.86, p = 0.001, respectively) regarding evidence-based practice terminology. Furthermore, place of training (trained overseas) was associated with a positive attitude towards literature findings (t = 2.26, p = 0.03). The results of this study demonstrated that participants reported that evidence-based practice had not been extensively implemented, despite positive attitudes regarding its implementation among physiotherapists practicing in Saudi Arabia.
Vitamin A as a Transcriptional Regulator of Cardiovascular Disease
Authors: Leigh Robert S. , Kaynak Bogac L.
Published: September 2020

Vitamin A is a micronutrient and signaling molecule that regulates transcription, cellular differentiation, and organ homeostasis. Additionally, metabolites of Vitamin A are utilized as differentiation agents in the treatment of hematological cancers and skin disorders, necessitating further study into the effects of both nutrient deficiency and the exogenous delivery of Vitamin A and its metabolites on cardiovascular phenotypes. Though vitamin A/retinoids are well-known regulators of cardiac formation, recent evidence has emerged that supports their role as regulators of cardiac regeneration, postnatal cardiac function, and cardiovascular disease progression. We here review findings from genetic and pharmacological studies describing the regulation of both myocyte- and vascular-driven cardiac phenotypes by vitamin A signaling. We identify the relationship between retinoids and maladaptive processes during the pathological hypertrophy of the heart, with a focus on the activation of neurohormonal signaling and fetal transcription factors (Gata4, Tbx5). Finally, we assess how this information might be leveraged to develop novel therapeutic avenues.
Sappanone A Prevents Left Ventricular Dysfunction in a Rat Myocardial Ischemia Reperfusion Injury Model
Authors: Jo Woori , Min Byung Sun , Yang Hee-Young , Park Na-Hye , Kang Kyung-Ku , Lee Sijoon , Chae Sehyun , Ma Eun Sook , Son Woo-Chan
Published: September 2020

The incidence of myocardial infarction, among the causes of cardiovascular morbidity and mortality, is increasing globally. In this study, left ventricular (LV) dysfunction, including LV systolic and diastolic function, was investigated in a rat myocardial ischemia/reperfusion injury model with echocardiography. The homoisoflavanone sappanone A is known for its anti-inflammatory effects. Using echocardiography, we found that sappanone A administration significantly improved LV systolic and diastolic function in a rat myocardial ischemia/reperfusion injury model, especially in the early phase development of myocardial infarction. Based on myocardial infarct size, serum cardiac marker assay, and histopathological evaluation, sappanone A showed higher efficacy at the doses used in our experiments than curcumin and was evaluated for its potential to improve LV function.
Heme Oxygenase-1 Supports Mitochondrial Energy Production and Electron Transport Chain Activity in Cultured Lung Epithelial Cells
Authors: Carr Jennifer F. , Garcia David , Scaffa Alejandro , Peterson Abigail L. , Ghio Andrew J. , Dennery Phyllis A.
Published: September 2020

Heme oxygenase-1 is induced by many cellular stressors and catalyzes the breakdown of heme to generate carbon monoxide and bilirubin, which confer cytoprotection. The role of HO-1 likely extends beyond the simple production of antioxidants, for example HO-1 activity has also been implicated in metabolism, but this function remains unclear. Here we used an HO-1 knockout lung cell line to further define the contribution of HO-1 to cellular metabolism. We found that knockout cells exhibit reduced growth and mitochondrial respiration, measured by oxygen consumption rate. Specifically, we found that HO-1 contributed to electron transport chain activity and utilization of certain mitochondrial fuels. Loss of HO-1 had no effect on intracellular non-heme iron concentration or on proteins whose levels and activities depend on available iron. We show that HO-1 supports essential functions of mitochondria, which highlights the protective effects of HO-1 in diverse pathologies and tissue types. Our results suggest that regulation of heme may be an equally significant role of HO-1.
New Factors Enhancing the Reactivity of Cysteines in Molten Globule-Like Structures
Authors: Gambardella Giorgia , Cattani Giada , Bocedi Alessio , Ricci Giorgio
Published: September 2020

Protein cysteines often play crucial functional and structural roles, so they are emerging targets to design covalent thiol ligands that are able to modulate enzyme or protein functions. Some of these residues, especially those involved in enzyme mechanisms—including nucleophilic and reductive catalysis and thiol-disulfide exchange—display unusual hyper-reactivity; such a property is expected to result from a low pKa and from a great accessibility to a given reagent. New findings and previous evidence clearly indicate that pKa perturbations can only produce two–four-times increased reactivity at physiological pH values, far from the hundred and even thousand-times kinetic enhancements observed for some protein cysteines. The data from the molten globule-like structures of ribonuclease, lysozyme, bovine serum albumin and chymotrypsinogen identified new speeding agents, i.e., hydrophobic/electrostatic interactions and productive complex formations involving the protein and thiol reagent, which were able to confer exceptional reactivity to structural cysteines which were only intended to form disulfides. This study, for the first time, evaluates quantitatively the different contributions of pKa and other factors to the overall reactivity. These findings may help to clarify the mechanisms that allow a rapid disulfide formation during the oxidative folding of many proteins.
Characterization of a Topically Testable Model of Burn Injury on Human Skin Explants
Authors: Gross-Amat Olivia , Guillen Marine , Salmon Damien , Nataf Serge , Auxenfans Céline
Published: September 2020

Severe burn injuries remain a major health problem due to high rates of mortality, residual morbidity, and/or aesthetic damages. To find new therapies aimed at promoting a harmonious healing of skin burns, it is important to develop models which take into account the unique properties of the human skin. Based on previously described models of burn injury performed on human skin explants, we hypothesized that maintaining explants under constant tension forces would allow to more closely reproduce the pathophysiological processes of skin remodeling. We thus. Here, we set up and characterized an improved model of deep second-degree burn injury on ex vivo cultured human skin explants at air-liquid interface and maintained under conditions of constant tension forces. A spontaneous re-epithelialization of the lesion was observed 8 to 9 days post burn and was found to rely on the proliferation of basal keratinocytes at the wound edges. Collagen VII at the dermo-epidermal junction reformed along with the progression of re-epithelializatio and a synthesis of procollagen III was observed in the dermis at the wound site. These findings indicate that our model is suitable for the assessment of clinically-relevant therapies aimed at modulating the kinetics of re-epithelialization and/or the activation of fibroblasts following skin burn injuries. In this regard, we evaluated the use of a thermoreversible poloxamer hydrogel as a vehicle for topically-testable therapeutic molecules. Our data showed that, although useful for drug formulation, the p407/p188 poloxamer hydrogel induces a delay of skin re-epithelialization in humans skin explants submitted to experimental burn injury.
HSPA1L Enhances Cancer Stem Cell-Like Properties by Activating IGF1Rβ and Regulating β-Catenin Transcription
Authors: Choi Soo-Im , Lee Jei-Ha , Kim Rae-Kwon , Jung Uhee , Kahm Yeon-Jee , Cho Eun-Wie , Kim In-Gyu
Published: September 2020

Studies have shown that cancer stem cells (CSCs) are involved in resistance and metastasis of cancer; thus, therapies targeting CSCs have been proposed. Here, we report that heat shock 70-kDa protein 1-like (HSPA1L) is partly involved in enhancing epithelial–mesenchymal transition (EMT) and CSC-like properties in non-small cell lung cancer (NSCLC) cells. Aldehyde dehydrogenase 1 (ALDH1) is considered a CSC marker in some lung cancers. Here, we analyzed transcriptional changes in genes between ALDH1high and ALDH1low cells sorted from A549 NSCLC cells and found that HSPA1L was highly expressed in ALDH1high cells. HSPA1L played two important roles in enhancing CSC-like properties. First, HSPA1L interacts directly with IGF1Rβ and integrin αV to form a triple complex that is involved in IGF1Rβ activation. HSPA1L/integrin αV complex-associated IGF1Rβ activation intensified the EMT-associated cancer stemness and γ-radiation resistance through its downstream AKT/NF-κB or AKT/GSK3β/β-catenin activation pathway. Secondly, HSPA1L was also present in the nucleus and could bind directly to the promoter region of β-catenin to function as a transcription activator of β-catenin, an important signaling protein characterizing CSCs by regulating ALDH1 expression. HSPA1L may be a novel potential target for cancer treatment because it both enhances IGF1Rβ activation and regulates γβ-catenin transcription, accumulating CSC-like properties.
Self-Assembly of Amphiphilic Compounds as a Versatile Tool for Construction of Nanoscale Drug Carriers
Authors: Kashapov Ruslan , Gaynanova Gulnara , Gabdrakhmanov Dinar , Kuznetsov Denis , Pavlov Rais , Petrov Konstantin , Zakharova Lucia , Sinyashin Oleg
Published: September 2020

This review focuses on synthetic and natural amphiphilic systems prepared from straight-chain and macrocyclic compounds capable of self-assembly with the formation of nanoscale aggregates of different morphology and their application as drug carriers. Since numerous biological species (lipid membrane, bacterial cell wall, mucous membrane, corneal epithelium, biopolymers, e.g., proteins, nucleic acids) bear negatively charged fragments, much attention is paid to cationic carriers providing high affinity for encapsulated drugs to targeted cells. First part of the review is devoted to self-assembling and functional properties of surfactant systems, with special attention focusing on cationic amphiphiles, including those bearing natural or cleavable fragments. Further, lipid formulations, especially liposomes, are discussed in terms of their fabrication and application for intracellular drug delivery. This section highlights several features of these carriers, including noncovalent modification of lipid formulations by cationic surfactants, pH-responsive properties, endosomal escape, etc. Third part of the review deals with nanocarriers based on macrocyclic compounds, with such important characteristics as mucoadhesive properties emphasized. In this section, different combinations of cyclodextrin platform conjugated with polymers is considered as drug delivery systems with synergetic effect that improves solubility, targeting and biocompatibility of formulations.
Integrated Question-Answering System for Natural Disaster Domains Based on Social Media Messages Posted at the Time of Disaster
Authors: Kemavuthanon Kemachart , Uchida Osamu
Published: September 2020

Natural disasters are events that humans cannot control, and Japan has suffered from many such disasters over its long history. Many of these have caused severe damage to human lives and property. These days, numerous Japanese people have gained considerable experience preparing for disasters and are now striving to predict the effects of disasters using social network services (SNSs) to exchange information in real time. Currently, Twitter is the most popular and powerful SNS tool used for disaster response in Japan because it allows users to exchange and disseminate information quickly. However, since almost all of the Japanese-related content is also written in the Japanese language, which restricts most of its benefits to Japanese people, we feel that it is necessary to create a disaster response system that would help people who do not understand Japanese. Accordingly, this paper presents the framework of a question-answering (QA) system that was developed using a Twitter dataset containing more than nine million tweets compiled during the Osaka North Earthquake that occurred on 18 June 2018. We also studied the structure of the questions posed and developed methods for classifying them into particular categories in order to find answers from the dataset using an ontology, word similarity, keyword frequency, and natural language processing. The experimental results presented herein confirm the accuracy of the answer results generated from our proposed system.
Maintenance Modelling of Ceramic Claddings in Pitched Roofs Based on the Evaluation of Their In Situ Degradation Condition
Authors: Ferreira Cláudia , Silva Ana , Brito Jorge de , S. Dias Ilídio , Flores-Colen Inês
Published: September 2020

Existing maintenance policies have several limitations, mainly due to the lack of knowledge regarding the durability and performance of buildings. Usually, the maintenance policies are insufficiently accurate, neglecting the risk of failure over time and the global costs associated with repairs. In this study, a condition-based maintenance model, based on Petri nets, is proposed to evaluate the impact of three maintenance strategies of ceramic claddings in pitched roofs (CCPR): MS1—only total replacement; MS2—composed of total replacement and minor intervention and MS3—composed of total replacement, minor intervention and cleaning operations. In this study, 146 CCPR were inspected in situ, with a total area of 43,991.6 m2. The remaining service life of the CCPR; the global costs over the claddings’ lifetime (considering inspection, maintenance, replacement and disposal costs); the claddings’ degradation condition and the number of replacements during the time horizon are used to evaluate the performance of the different maintenance strategies through a simplified multi-criteria analysis. The results show that the gains in performance, in terms of expected service life and durability, of the consideration of preventive maintenance actions (minor interventions or cleaning operations) outweigh the increase of the operation costs.
Retrospective Analysis of a Modified Organizational Model to Guarantee CT Workflow during the COVID-19 Outbreak in the Tertiary Hospital of Padova, Italy
Authors: Cester Giacomo , Giraudo Chiara , Causin Francesco , Boemo Deris Gianni , Anglani Mariagiulia , Capizzi Alfio , Carretta Giovanni , Cattelan Annamaria , Cecchin Diego , Cianci Vito , Crisanti Andrea , De Conti Giorgio , Donato Daniele , Flor Luciano , Gabrieli Joseph-Domenico , Munari Marina , Navalesi Paolo , Ponzoni Alberto , Scapellato Maria Luisa , Tiberio Ivo , Vianello Andrea , Stramare Roberto
Published: September 2020

At the beginning of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) outbreak in Italy, the cluster of Vò Euganeo was managed by the University Hospital of Padova. The Department of Diagnostic Imaging (DDI) conceived an organizational approach based on three different pathways for low-risk, high-risk, and confirmed Coronavirus Disease 19 (COVID-19) patients to accomplish three main targets: guarantee a safe pathway for non-COVID-19 patients, ensure health personnel safety, and maintain an efficient workload. Thus, an additional pathway was created with the aid of a trailer-mounted Computed Tomography (CT) scanner devoted to positive patients. We evaluated the performance of our approach from February 21 through April 12 in terms of workload (e.g., number of CT examinations) and safety (COVID-19-positive healthcare workers). There was an average of 72.2 and 17.8 COVID-19 patients per day in wards and the Intensive Care Unit (ICU), respectively. A total of 176 high-risk and positive patients were examined. High Resolution Computed Tomography (HRCT) was one of the most common exams, and 24 pulmonary embolism scans were performed. No in-hospital transmission occurred in the DDI neither among patients nor among health personnel. The weekly number of in-patient CT examinations decreased by 27.4%, and the surgical procedures decreased by 29.5%. Patient screening and dedicated diagnostic pathways allowed the maintenance of high standards of care while working in safety.
Bilateral Subthalamic Nucleus Deep Brain Stimulation under General Anesthesia: Literature Review and Single Center Experience
Authors: Park Hye Ran , Lim Yong Hoon , Song Eun Jin , Lee Jae Meen , Park Kawngwoo , Park Kwang Hyon , Lee Woong-Woo , Kim Han-Joon , Jeon Beomseok , Paek Sun Ha
Published: September 2020

Bilateral subthalamic nucleus (STN) Deep brain stimulation (DBS) is a well-established treatment in patients with Parkinson’s disease (PD). Traditionally, STN DBS for PD is performed by using microelectrode recording (MER) and/or intraoperative macrostimulation under local anesthesia (LA). However, many patients cannot tolerate the long operation time under LA without medication. In addition, it cannot be even be performed on PD patients with poor physical and neurological condition. Recently, it has been reported that STN DBS under general anesthesia (GA) can be successfully performed due to the feasible MER under GA, as well as the technical advancement in direct targeting and intraoperative imaging. The authors reviewed the previously published literature on STN DBS under GA using intraoperative imaging and MER, focused on discussing the technique, clinical outcome, and the complication, as well as introducing our single-center experience. Based on the reports of previously published studies and ours, GA did not interfere with the MER signal from STN. STN DBS under GA without intraoperative stimulation shows similar or better clinical outcome without any additional complication compared to STN DBS under LA. Long-term follow-up with a large number of the patients would be necessary to validate the safety and efficacy of STN DBS under GA.
Bio-Based Electrospun Fibers for Wound Healing
Authors: Azimi Bahareh , Maleki Homa , Zavagna Lorenzo , De la Ossa Jose Gustavo , Linari Stefano , Lazzeri Andrea , Danti Serena
Published: September 2020

Being designated to protect other tissues, skin is the first and largest human body organ to be injured and for this reason, it is accredited with a high capacity for self-repairing. However, in the case of profound lesions or large surface loss, the natural wound healing process may be ineffective or insufficient, leading to detrimental and painful conditions that require repair adjuvants and tissue substitutes. In addition to the conventional wound care options, biodegradable polymers, both synthetic and biologic origin, are gaining increased importance for their high biocompatibility, biodegradation, and bioactive properties, such as antimicrobial, immunomodulatory, cell proliferative, and angiogenic. To create a microenvironment suitable for the healing process, a key property is the ability of a polymer to be spun into submicrometric fibers (e.g., via electrospinning), since they mimic the fibrous extracellular matrix and can support neo- tissue growth. A number of biodegradable polymers used in the biomedical sector comply with the definition of bio-based polymers (known also as biopolymers), which are recently being used in other industrial sectors for reducing the material and energy impact on the environment, as they are derived from renewable biological resources. In this review, after a description of the fundamental concepts of wound healing, with emphasis on advanced wound dressings, the recent developments of bio-based natural and synthetic electrospun structures for efficient wound healing applications are highlighted and discussed. This review aims to improve awareness on the use of bio-based polymers in medical devices.
Application of the SOSim v2 Model to Spills of Sunken Oil in Rivers
Authors: Jacketti Mary , Englehardt James D. , Beegle-Krause C.J.
Published: September 2020

Sunken oil transport processes in rivers differ from those in oceans, and currently available models may not be generally applicable to sunken oil in river settings. The open-source Subsurface Oil Simulator (SOSim) model has been expanded to handle spills of sunken oil in navigable rivers, utilizing Bayesian inference to integrate field concentration data with bathymetric data to predict the location and movement of sunken oil. A novel prior likelihood function incorporates bathymetric input, with sampling grid and default parameters adapted appropriately for rivers. SOSim v2 was demonstrated versus field observations taken following the M/T (Motor Tanker) Athos I oil spill. The model was also modified to operate in 1-D, to assess the longitudinal distribution of sunken oil in a non-navigable river using available poling data collected following the Enbridge Kalamazoo River oil spill in 2010. Results of both case studies were consistent with observed data and local bathymetry in 2-D and 1-D, and the model is suggested as a complement to deterministic models for oil spill emergency response in rivers.
On the Sensitivity of Typhoon Wave Simulations to Tidal Elevation and Current
Authors: Hsiao Shih-Chun , Wu Han-Lun , Chen Wei-Bo , Chang Chih-Hsin , Lin Lee-Yaw
Published: September 2020

The sensitivity of storm wave simulations to storm tides and tidal currents was investigated using a high-resolution, unstructured-grid, coupled circulation-wave model (Semi-implicit Cross-scale Hydroscience Integrated System Model Wind Wave Model version III (SCHISM-WWM-III)) driven by two typhoon events (Typhoons Soudelor and Megi) impacting the northeastern coast of Taiwan. Hourly wind fields were acquired from a fifth-generation global atmospheric reanalysis (ERA5) and were used as meteorological conditions for the circulation-wave model after direct modification (MERA5). The large typhoon-induced waves derived from SCHISM-WWM-III were significantly improved with the MERA5 winds, and the peak wave height was increased by 1.0–2.0 m. A series of numerical experiments were conducted with SCHISM-WWM-II and MERA5 to explore the responses of typhoon wave simulations to tidal elevation and current. The results demonstrate that the simulated significant wave height, mean wave period and wave direction for a wave buoy in the outer region of the typhoon are more sensitive to the tidal current but less sensitive to the tidal elevation than those for a wave buoy moored in the inner region of the typhoon. This study suggests that the inclusion of the tidal current and elevation could be more important for typhoon wave modeling in sea areas with larger tidal ranges and higher tidal currents. Additionally, the suitable modification of the typhoon winds from a global atmospheric reanalysis is necessary for the accurate simulation of storm waves over the entire region of a typhoon.
Designed Antimicrobial Peptides Against Trauma-Related Cutaneous Invasive Fungal Wound Infections
Authors: Woodburn Kathryn W. , Jaynes Jesse M. , Clemens L. Edward
Published: September 2020

Cutaneous invasive fungal wound infections after life-threatening dismounted complex blast injury (DCBI) and natural disasters complicate clinical care. These wounds often require aggressive repeated surgical debridement, can result in amputations and hemipelvectomies and have a 38% mortality rate. Given the substantial morbidity associated with cutaneous fungal wound infections, patients at risk need immediate empiric treatment mandating the use of rapidly acting broad-spectrum antimicrobials, acting on both fungi and bacteria, that are also effective against biofilm and can be administered topically. Designed antimicrobial peptides (dAMPs) are engineered analogues of innate antimicrobial peptides which provide the first line of defense against invading pathogens. The antifungal and antibacterial effect and mammalian cytotoxicity of seven innovative dAMPs, created by iterative structural analog revisions and physicochemical and functional testing were investigated. The dAMPs possess broad-spectrum antifungal activity, in addition to being effective against Gram-negative and Gram-positive bacteria, which is crucial as many wounds are polymicrobial and require immediate empiric treatment. Three of the most potent dAMPs—RP504, RP556 and RP557—possess limited mammalian cytotoxicity following 8 h incubation. If these encouraging broad-spectrum antimicrobial and rapid acting results are translated clinically, these novel dAMPs may become a first line empiric topical treatment for traumatic wound injuries.
Capital Structure Choices in Technology Firms: Empirical Results from Polish Listed Companies
Authors: Kedzior Marcin , Grabinska Barbara , Grabinski Konrad , Kedzior Dorota
Published: September 2020

The main aim of the paper is the identification of capital structure determinants, with a special emphasis on investments in the innovativeness of Polish New Technology-Based Firms (NTBFs). Poland is a unique country in that it is an emerging market that was also promoted in 2018 to the status of a developed country. The study sample consisted of 31 companies listed in the Warsaw Stock Exchange that are classified as high-tech firms and covers the period 2014–2018. The following factors influencing the capital structure were analyzed: internal and external innovativeness and the firm’s size, liquidity, intangibility, age, profitability, and growth opportunities. The results of the research provide empirical evidence that liquidity, age, and investments in innovativeness determine capital structure, which provides an additional argument supporting the trade-off theory and the modified version of the pecking order theory. More specifically, the results suggest that companies whose process of investment in innovativeness is based on the external acquisition of technology are able to attract external financing, while the process based on internally generated innovativeness (R&D activity) deters external capital. The results are interesting for policymakers in emerging markets.
A Review of Changes in Mountain Land Use and Ecosystem Services: From Theory to Practice
Authors: Pătru-Stupariu Ileana , Hossu Constantina Alina , Grădinaru Simona Raluca , Nita Andreea , Stupariu Mihai-Sorin , Huzui-Stoiculescu Alina , Gavrilidis Athanasios-Alexandru
Published: September 2020

Global changes impact the human-environment relationship, and, in particular, they affect the provision of ecosystem services. Mountain ecosystems provide a wide range of such services, but they are highly sensitive and vulnerable to change due to various human pressures and natural processes. We conducted a literature survey that focused on two main issues. The first was the identification of quantitative methods aimed at assessing the impact of land use changes in mountain regions and the related ecosystem services. The second was the analysis of the extent to which the outcomes of these assessments are useful and transferable to stakeholders. We selected papers through a keyword-driven search of the ISI Web of Knowledge and other international databases. The keywords used for the search were mountain land use change and ecosystem service. Quantitative approaches to ecosystem service assessment rely on suitable indicators, therefore land use/land cover can be used as an appropriate proxy. Landscape metrics are a powerful analytical tool; their use can increase the accuracy of assessments and facilitate the mitigation of specific phenomena, such as fragmentation or the reduction of core habitat areas. Mapping is essential: it is the basis for spatial analyzes and eases the interactions between stakeholders. Land use/land cover change is a temporal process, so both past and future approaches are meaningful. It is necessary to enhance information transfer from theory to practice. Increasing stakeholder awareness can lead to suitable management solutions, and, reciprocally, stakeholder feedback can help improve current assessment methodologies and contribute to developing new tools that are suitable for specific problems.
Effects of Land Urbanization on Smog Pollution in China: Estimation of Spatial Autoregressive Panel Data Models
Authors: Yu Xuan , Shen Manhong , Shen Weiteng , Zhang Xiao
Published: September 2020

Studying the impact of land urbanization on smog pollution has important guiding significance for the sustainable development of cities. This study adds the spatial effect between regions into the research framework of smog pollution control in China. On the basis of a panel dataset of 31 province-level administrative regions in China from 2000 to 2017, we investigate the impact of land urbanization on smog pollution. We construct a spatial weight matrix and use Moran’s I statistic and the spatial autoregressive panel data model. The research results show that land urbanization and smog pollution have an inverted U-shaped relationship. With the advancement of land urbanization, the area’s smog pollution first increases and then decreases. However, in general, China has not passed the inflection point and is still at a stage where increasing land urbanization rate aggravates smog pollution. Moreover, the country’s smog pollution has a significant spatial positive correlation that shows agglomeration. In that context, multiple environmental governance entities, including the government, enterprises, and the public, need to collaborate on measures to reduce smog pollution. Future urban construction in China will need to integrate solutions that address the current nexus between urbanization and smog pollution to achieve green and sustainable development.
Meta-Analysis of Geomorphodynamics in the Western Lower Bakırçay Plain (Aegean Region, Turkey)
Authors: Becker Fabian , Knitter Daniel , Nykamp Moritz , Schütt Brigitta
Published: September 2020

The relation between human activities, climate variability, and geomorphodynamics in the Mediterranean region is widely discussed. For the western lower Bakırçay plain in the ancient Pergamon Micro-Region, geoarchaeological studies have shown changes in geomorphodynamics primarily on a site-basis. We reconstruct past geomorphodynamics in the area based on a meta-analysis of 108 14C-ages obtained from 25 sediment sequences mainly from colluvial and alluvial deposits by analyzing cumulative probability functions of the 14C-ages. Accounting for biases in the database, we applied different approaches and compared the empirical probability functions with simulated functions. Reconstructed geomorphodynamics in the western lower Bakırçay plain during the Holocene principally coincide with a trend of climate-driven sensitivity to erosion and population dynamics in the eastern Mediterranean, but are also related to the local settlement history. Our data analysis shows that transformations of the Pergamon Micro-Region between the Hellenistic and Roman Imperial times is contemporary to increasing geomorphodynamics that peak in Roman Imperial times. However, a cause–effect relationship between geomorphodynamics and settlement dynamics should be further evaluated. A comparison with data from other settlement centers in Anatolia shows that a coincidence between the peak in geomorphodynamics and a peak in settlement activity are not obvious and may be influenced by soil conservation measures, preferred settlement location, and inherited soil exhaustion.
Case Report: Identification of a Novel Variant (m.8909T>C) of Human Mitochondrial ATP6 Gene and Its Functional Consequences on Yeast ATP Synthase
Authors: Ding Qiuju , Kucharczyk Róża , Zhao Weiwei , Dautant Alain , Xu Shutian , Niedzwiecka Katarzyna , Su Xin , Giraud Marie-France , Gombeau Kewin , Zhang Mingchao , Xie Honglang , Zeng Caihong , Bouhier Marine , di Rago Jean-Paul , Liu Zhihong , Tribouillard-Tanvier Déborah , Chen Huimei
Published: September 2020

With the advent of next generation sequencing, the list of mitochondrial DNA (mtDNA) mutations identified in patients rapidly and continuously expands. They are frequently found in a limited number of cases, sometimes a single individual (as with the case herein reported) and in heterogeneous genetic backgrounds (heteroplasmy), which makes it difficult to conclude about their pathogenicity and functional consequences. As an organism amenable to mitochondrial DNA manipulation, able to survive by fermentation to loss-of-function mtDNA mutations, and where heteroplasmy is unstable, Saccharomyces cerevisiae is an excellent model for investigating novel human mtDNA variants, in isolation and in a controlled genetic context. We herein report the identification of a novel variant in mitochondrial ATP6 gene, m.8909T>C. It was found in combination with the well-known pathogenic m.3243A>G mutation in mt-tRNALeu. We show that an equivalent of the m.8909T>C mutation compromises yeast adenosine tri-phosphate (ATP) synthase assembly/stability and reduces the rate of mitochondrial ATP synthesis by 20–30% compared to wild type yeast. Other previously reported ATP6 mutations with a well-established pathogenicity (like m.8993T>C and m.9176T>C) were shown to have similar effects on yeast ATP synthase. It can be inferred that alone the m.8909T>C variant has the potential to compromise human health.
Simulation Analysis of Erosion–Corrosion Behaviors of Elbow under Gas-Solid Two-Phase Flow Conditions
Authors: Zeng Qunfeng , Qi Wenchuang
Published: September 2020

In the production and gathering process of coal gas, the complex composition of the coal gas, harsh environments, the complex medium, and high content of solid particles in slurry cause the equipment malfunctions and even failure because of erosion and corrosion. In the present study, COMSOL multi-physics finite element simulation software is used to simulate the erosion–corrosion behaviors of elbow in key chemical equipments. The electrochemical corrosion, solid particle erosion, chemical reaction, and turbulent flow are coupled together. The particle count method is proposed to clarify the erosion phenomenon. The simulation results show that particles with high turbulent intensity hit the wall of elbow directly, which forms a slanted elliptical erosion zone on the extrados surface at 40°–50°. The chemical reaction in turbulence has a difference in the concentration distribution of substances, and this phenomenon leads to different magnitudes of the corrosion current densities in the tube. Moreover, 1/6 released particles hit the extrados surface of the elbow. These findings are beneficial to understand the erosion–corrosion phenomena and design the elbow in key chemical equipment.