bluuebunny/crossref_metadata_2025 huggingface dataset:
Midazolam Ameliorates Acute Liver Injury Induced by Carbon Tetrachloride via Enhancing Nrf2 Signaling Pathway
Authors: Zhang Yongyan , Zhu Yadi , Li Ying , Ji Feng , Ge Guangbo , Xu Hua
Published: July 2022

Oxidative stress contributes greatly to initiation and progression of liver injury. Activation of nuclear-factor erythroid 2-related factor 2 (Nrf2) has been considered as an attractive strategy for preventing and treating the oxidative damage related to liver injury. This study aimed to find an efficacious agent to activate Nrf2/HO-1 signaling pathway from clinically used therapeutic agents and to characterize the usefulness for preventing and treating CCl4-induced acute liver injury. For this purpose, a series of clinically used therapeutic agents were collected and their activation potentials on Nrf2 were assayed by using 293T-Nrf2-luc cell line. Among all tested therapeutic agents, midazolam was found with good Nrf2 activation effect and this agent could significantly ameliorate CCl4-induced damage to HepG2 cells. In vivo animal tests showed that pretreatment with midazolam reduced the liver pathological tissue damage and the serum levels of ALT and AST in CCl4-induced liver injury mice. Further investigations showed that midazolam could strongly up-regulate the expression of both Nrf2 and HO-1 in the mice liver, accompanied by increasing of the levels of antioxidant enzyme SOD and reducing the production of MDA, as well as reducing the pro-inflammatory cytokines (IL-6, TNF-α) secretion. Collectively, our results clearly demonstrate that midazolam can ameliorate CCl4-induced acute liver injury and oxidative stress via activating the Nrf2 signaling pathway.
Pharmacokinetic Differences of Wuji Pill Components in Normal and Chronic Visceral Hypersensitivity Irritable Bowel Syndrome Rats Attributable to Changes in Tight Junction and Transporters
Authors: Gong Zipeng , Yang Qing , Wang Yajie , Weng Xiaogang , Li Yujie , Dong Yu , Zhu Xiaoxin , Chen Ying
Published: July 2022

The Wuji pill, also called Wuji Wan (WJW), is an effective traditional medicine for the clinical treatment of irritable bowel syndrome (IBS). It is principally composed of Rhizoma Coptidis, Fructus Evodiae Rutaecarpae, and Radix Paeoniae Alba. There have been no reports on the pharmacokinetics of WJW on IBS. Because it is more meaningful to study pharmacokinetics in relation to specific pathological conditions, our study investigated the pharmacokinetic differences of five representative components (berberine, palmatine, evodiamine, rutaecarpine, and paeoniflorin) in normal rats and chronic visceral hypersensitivity IBS (CVH-IBS) model rats after single dose and multiple doses of WJW using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Transmission electron microscopy, immunohistochemistry, and immunofluorescence were used to explore mechanisms behind the pharmacokinetic differences in terms of tight junction proteins (Occludin and ZO-1), myosin light chain kinase (MLCK), and transporters including P-glycoprotein (P-gp), multidrug resistance associated protein 1 (MRP1), and multidrug resistance associated protein 2 (MRP2) in rat colons. After a single dose, for all components except rutaecarpine, significant differences were observed between normal and model groups. Compared with normal group, T1/2 and AUC0-t of berberine and palmatine in model group increased significantly (562.5 ± 237.2 vs. 1,384.9 ± 712.4 min, 733.8 ± 67.4 vs. 1,532.4 ± 612.7 min; 5,443.0 ± 1,405.8 vs. 9,930.8 ± 2,304.5 min·ng/ml, 2,365.5 ± 410.6 vs. 3,527.0 ± 717.8 min·ng/ml), while Cl/F decreased (840.7 ± 250.8 vs. 397.3 ± 142.7 L/h/kg, 427.7 ± 89.4 vs. 288.9 ± 114.4 L/h/kg). Cmax and AUC0-t of evodiamine in model group increased significantly (1.4 ± 0.6 vs. 2.4 ± 0.7 ng/ml; 573 ± 45.3 vs. 733.9 ± 160.2 min·ng/ml), while T1/2, Tmax, Cl/F, and Vd/F had no significant difference. Tmax and AUC0-t of paeoniflorin in model group increased significantly (21.0 ± 8.2 vs. 80.0 ± 45.8 min; 15,428.9 ± 5,063.6 vs. 33,140.6 ± 5,613.9 min·ng/ml), while Cl/F decreased (110.5 ± 48.1 vs. 43.3 ± 9.5 L/h/kg). However, after multiple doses, all five components showed significant differences between normal and model groups. Moreover, these differences were related to tight junction damage and the differential expression of transporters in the colon, suggesting that dose adjustment might be required during administration of WJW in the clinical treatment of IBS.
ICT-Based Individualized Training of Institutionalized Individuals With Dementia. Evaluation of Usability and Trends Toward the Effectiveness of the InCoPE-App
Authors: Barisch-Fritz Bettina , Bezold Jelena , Scharpf Andrea , Trautwein Sandra , Krell-Roesch Janina , Woll Alexander
Published: July 2022

Physical activity interventions can alleviate the course of disease for individuals with dementia (IWD) who have been extraordinarily affected by the COVID-19 pandemic. Information and Communication Technology (ICT) provides new opportunities not only to mitigate negative effects of the pandemic but also to sustainably improve everyday life of IWD in nursing homes. The aim of the present study was to evaluate the ICT-based InCoPE-App, which was used to assess physical and cognitive performance and deliver individualized exercise for IWD, with regard to 1) user experience of nursing assistants, and 2) trends toward the effectiveness of the intervention on physical and cognitive performance of IWD. An 18-week individualized multidomain intervention (2 × 60 min/session) was delivered to an intervention group (IG; n = 10, mean age 88.4 ± 5.6, 70% female) by nursing assistants (n = 10, mean age 56.1 ± 10.4, 90% female) using the InCoPE-App. A control group (CG; n = 3, mean age 87.3 ± 3.5, 100% female) received conventional treatment. User experience was assessed among nursing assistants by different questionnaires, i.e., PSSUQ and ISONORM 9241/110-S for usability, and AttrakDiff2 for pragmatic (PQ), hedonic quality-identity and stimulation (HQI and HQS), and attractiveness (ATT). Trends toward the effectiveness of the intervention were assessed using MMSE (global cognitive function), FICSIT-4 (balance), 6MWT and TUG (mobility), and m30CST (function of lower limbs). Usability of the InCoPE-App was rated as high by nursing assistants (mean ± SD; overall PSSUQ 2.11 ± 0.75; overall ISONORM 9241/110-S 1.90 ± 0.88; ATT 1.86 ± 1.01; PQ 1.79 ± 1.03; HQI 1.8 ± 0.79; and HQS 1.37 ± 0.69). Dropout was high in the total sample (36.7%). Trends toward the effectiveness were observed within IG in nine IWD who showed positive or neutral trends in at least two physical performance outcomes. Seven participants had positive or neutral trends in the FICSIT-4, seven participants in m30CST, and four and seven participants in 6MWT and TUG, respectively. In conclusion, the InCoPE-App has good nursing assistant-rated usability, whereas training effects and intervention adherence were rather low most likely due to COVID-19 restrictions. Single-subject research revealed more positive than negative trends in IG of IWD. Further research is needed to evaluate feasibility, suitability, and effectiveness of the InCoPE-App.
Molecular Pathways and Key Genes Associated With Breast Width and Protein Content in White Striping and Wooden Breast Chicken Pectoral Muscle
Authors: Bordini Martina , Soglia Francesca , Davoli Roberta , Zappaterra Martina , Petracci Massimiliano , Meluzzi Adele
Published: July 2022

Growth-related abnormalities affecting modern chickens, known as White Striping (WS) and Wooden Breast (WB), have been deeply investigated in the last decade. Nevertheless, their precise etiology remains unclear. The present study aimed at providing new insights into the molecular mechanisms involved in their onset by identifying clusters of co-expressed genes (i.e., modules) and key loci associated with phenotypes highly related to the occurrence of these muscular disorders. The data obtained by a Weighted Gene Co-expression Network Analysis (WGCNA) were investigated to identify hub genes associated with the parameters breast width (W) and total crude protein content (PC) of Pectoralis major muscles (PM) previously harvested from 12 fast-growing broilers (6 normal vs. 6 affected by WS/WB). W and PC can be considered markers of the high breast yield of modern broilers and the impaired composition of abnormal fillets, respectively. Among the identified modules, the turquoise (r = -0.90, p < 0.0001) and yellow2 (r = 0.91, p < 0.0001) were those most significantly related to PC and W, and therefore respectively named “protein content” and “width” modules. Functional analysis of the width module evidenced genes involved in the ubiquitin-mediated proteolysis and inflammatory response. GTPase activator activity, PI3K-Akt signaling pathway, collagen catabolic process, and blood vessel development have been detected among the most significant functional categories of the protein content module. The most interconnected hub genes detected for the width module encode for proteins implicated in the adaptive responses to oxidative stress (i.e., THRAP3 and PRPF40A), and a member of the inhibitor of apoptosis family (i.e., BIRC2) involved in contrasting apoptotic events related to the endoplasmic reticulum (ER)-stress. The protein content module showed hub genes coding for different types of collagens (such as COL6A3 and COL5A2), along with MMP2 and SPARC, which are implicated in Collagen type IV catabolism and biosynthesis. Taken together, the present findings suggested that an ER stress condition may underly the inflammatory responses and apoptotic events taking place within affected PM muscles. Moreover, these results support the hypothesis of a role of the Collagen type IV in the cascade of events leading to the occurrence of WS/WB and identify novel actors probably involved in their onset.
Effects of Anthropic Pollutants Identified in Pampas Lakes on the Development and Reproduction of Pejerrey Fish Odontesthes bonariensis
Authors: Miranda Leandro A. , Somoza Gustavo M.
Published: July 2022

Anthropic activities can seriously affect the health of the organisms inhabiting them, and the observation of any alteration in the reproduction of fish could be associated with the presence of endocrine disruptors. In this manuscript we have collected information on the adverse effects of pollutants (heavy metals, environmental steroids, and agrochemicals), present in Chascomús lake, Argentina, either at environmentally relevant and pharmacological concentrations on reproduction, embryonic development, and larval survival of pejerrey fish Odontesthes bonariensis. During development, it has been reported that 17β-estradiol (E2) feminized and reduced larval survival, while 17α-ethinyl-estradiol (EE2) not only feminized but also affected both embryo and larval survival. In adult male fish, treatments with EE2 and E2 + EE2 were able to increase mRNA abundance of gnrh3 and cyp19a1b and decreased those of gonadotropin receptors (fshr and lhcgr). Heavy metals such as cadmium, chromium, and copper negatively affected sperm quality, diminishing the motility. Also, a decrease in the percentage of hatching rate and larval survival was also observed with the same metals, highlighting zinc as the most detrimental metal. Furthermore, all these metals altered the expression of hypothalamic and pituitary genes related to reproduction in male pejerrey (gnrh1,2,3; cyp19a1b; fshb; lhb; fshr and, lhcgr). Moreover, in all cases pyknotic cells, corresponding to the degeneration of the germ cells, were observed in the testes of exposed fish. For agrochemicals, exposure of male pejerrey to environmental concentrations of glyphosate did not cause alterations on the endocrine reproductive axis. However, male pejerrey with gonadal abnormalities such as the presence of intersex (testis-ova) gonads were found in other Pampa´s lakes with high concentrations of atrazine and glyphosate associated with soybean and corn crops near their coasts. These types of studies demonstrate that pejerrey, an endemic species with economic importance inhabiting the Pampas shallow lakes, can be used as a sentinel species. It should be noted that increased pollution of aquatic ecosystems and the effects on the reproduction of organisms can lead to a decline in fish populations worldwide. Which, added to overfishing and other external factors such as global warming, could cause an eventual extinction of an emblematic species.
Transcriptome Approach Reveals the Response Mechanism of Heimia myrtifolia (Lythraceae, Myrtales) to Drought Stress
Authors: Lin Lin , Wang Jie , Wang Qun , Ji Mengcheng , Hong Sidan , Shang Linxue , Zhang Guozhe , Zhao Yu , Ma Qingqing , Gu Cuihua
Published: July 2022

Drought is a major environmental condition that inhibits the development and cultivation of Heimia myrtifolia. The molecular processes of drought resistance in H. myrtifolia remain unknown, which has limited its application. In our study, transcriptome analyzes were compared across three treatment groups (CK, T1, and T2), to investigate the molecular mechanism of drought resistance. Plant leaves wilted and drooped as the duration of drought stress increased. The relative water content of the leaves declined dramatically, and relative electrolyte leakage rose progressively. Using an RNA-Seq approach, a total of 62,015 unigenes with an average length of 1730 bp were found, with 86.61% of them annotated to seven databases, and 14,272 differentially expressed genes (DEGs) were identified in drought stress. GO and KEGG enrichment analyzes of the DEGs revealed significantly enriched KEGG pathways, including photosynthesis, photosynthetic antenna proteins, plant hormone signal transduction, glutathione metabolism, and ascorbate and aldarate metabolism. Abscisic acid signal transduction was the most prevalent in the plant hormone signal transduction pathway, and other plant hormone signal transductions were also involved in the drought stress response. The transcription factors (including MYB, NAC, WRKY, and bHLH) and related differential genes on significantly enriched pathways all played important roles in the drought process, such as photosynthesis-related genes and antioxidant enzyme genes. In conclusion, this study will provide several genetic resources for further investigation of the molecular processes that will be beneficial to H. myrtifolia cultivation and breeding.
Genetic Diversity of Transcription Factor Genes in Triticum and Mining for Promising Haplotypes for Beneficial Agronomic Traits
Authors: Yang Guang , Zhang Ying , Wei Xinyu , Cui Licao , Nie Xiaojun
Published: July 2022

Transcription factor (TF) is a class of the sequence-specific DNA-binding proteins that modulate the transcription of target genes, and thus regulate their expressions. Variations in TF are the crucial determinants for phenotypic traits. Although much progress has been made in the functions of TF genes in wheat, one of the most important staple crops globally, the diversity of TF genes in wheat and its progenitors are not well understood, especially the agronomically promising haplotypes have not yet been characterized. Here, we identified a total of 6,023 TF genes from hexaploid wheat through a genome-search method and classified them into 59 gene families based on the conserved domain. The characteristics and dN/dS values of these genes showed evidently selective effects. Based on re-sequencing data, we found a strong genetic bottleneck among these TF genes on A and D subgenomes while no found in B subgenome during wheat domestication. Combined with selective signals and known QTLs on the whole genome, 21 TF genes were preliminarily found to be associated with yield-related traits. The haplotype frequency of these TF genes was further investigated in bread wheat and its progenitors and 13 major haplotypes were the casual loci related to key traits. Finally, the tissue-specific TF genes were also identified using RNA-seq analysis. This study provided insights into the diversity and evolution of TF genes and the identified TF genes and excellent haplotypes associating with traits will contribute to wheat genetic improvement.
Anti-fungal Activity of Dalbergia retusa Extract on Gloeophyllum trabeum
Authors: Lv Huangfei , Wang Yulong , Qu Mingxuan , Zhang Yingying , Song Zhiru , Su Xingyu , Xu Bin
Published: July 2022

Hongmu is a type of material with strong corrosion resistance, and its extract has wood preservative properties in a variety of environmental settings. Herein, the processing residue of Dalbergia retusa was used to obtain an ethanol-extract, whose anti-fungal properties and mechanism was investigated using multi-omics principles and gas chromatography-mass spectrometry (GC-MS). The results show that D. retusa extract had a strong inhibitory effect on decaying fungus, and the inhibitory effect was dose dependent. High-throughput sequencing detected a total of 11,755 genes for transcriptome comparison. A total of 390 genes were differentially expressed, with 69 up-regulated and 321 down-regulated genes, indicating that D. retusa extract can significantly affect metabolic processes in decaying fungus. GC-MS results revealed that D. retusa extract was rich in phenols, ketones, amines, and aromatic compounds, which are likely to contribute to the excellent synergy between anti-fungal properties and anti-fungal activity (anti-fungal ability and active ingredients). In summary, this study describes the anti-fungal components in D. retusa extract, and our results provide a foundation for the study of their mechanism of action in this tree species.
The Moderating Effects of “Dark” Personality Traits and Message Vividness on the Persuasiveness of Terrorist Narrative Propaganda
Authors: Braddock Kurt , Schumann Sandy , Corner Emily , Gill Paul
Published: July 2022

Terrorism researchers have long discussed the role of psychology in the radicalization process. This work has included research on the respective roles of individual psychological traits and responses to terrorist propaganda. Unfortunately, much of this work has looked at psychological traits and responses to propaganda individually and has not considered how these factors may interact. This study redresses this gap in the literature. In this experiment (N = 268), participants were measured in terms of their narcissism, Machiavellianism, subclinical psychopathy, and everyday sadism—collectively called the Dark Tetrad. Participants were then exposed to a vivid or nonvivid terrorist narrative (or a control message). Results indicate that Machiavellianism interacts with both narrative exposure and narrative vividness to amplify the persuasive effect of terrorist narratives. Neither narcissism, subclinical psychopathy, nor everyday sadism had such an effect. These results highlight the importance of considering the psychological traits of audiences when evaluating proclivity for radicalizationviapersuasion by terrorist narratives.
Brokering or Sitting Between Two Chairs? A Group Perspective on Workplace Gossip
Authors: Estévez José Luis , Takács Károly
Published: July 2022

Brokerage is a central concept in the organization literature. It has been argued that individuals in broker positions—i.e., connecting otherwise disconnected parts within a firm’s social network—can control the flow of information. It would imply their increased relevance in workplace gossip. This allegation, however, has not been addressed empirically yet. To fill this gap, we apply social network analysis techniques to relational data from six organizations in Hungary. First, we identify informal groups and individuals in broker positions. Then, we use this information to predict the likelihood with which positive or negative gossip is reported. We find more gossip when the sender and receiver are part of the same group and more positive gossip about in-group rather than out-group targets. Individuals in broker positions are more likely the senders and targets of negative gossip. Finally, even if both the brokers and the boss(es) are the targets of their colleagues’ negative gossip, the combination of the two categories (bosses in broker positions) does not predict more negative gossip anymore. Results are discussed in relation to the theoretical accounts on brokerage that emphasize its power for information control but fail to recognize the pitfalls of being in such positions.
How Often Do You Think About Your Relationship With Nature? The Measurement of Environmental Identity Salience and Its Relationship With Proenvironmental Behaviors
Authors: Rahmani Leïla , Haasova Simona , Czellar Sandor , Clergue Valentina , Martin Christian
Published: July 2022

Extant research finds that environmental identity is an important motivational factor for proenvironmental behavior. However, studies typically focus on investigating the effects of the strength of this identity. Based on insights from identity research, we theorize that the influence of individuals’ environmental identity on their proenvironmental behavior may depend on other identity dimensions as well. We argue that the frequency of activation of environmental identity in relevant life domains—environmental identity salience—may predict proenvironmental behavior beyond what environmental identity strength can explain. To test our theorizing, we propose a parsimonious measure of environmental identity salience. In four empirical studies, we establish that the new measure has sound psychometric properties in terms of internal consistency and discriminant validity with regard to measures of environmental identity strength. Importantly, our measure of environmental identity salience reliably predicts a range of self-reported and actual proenvironmental behaviors beyond the effects of environmental identity strength. In line with theoretical predictions, our data suggests that environmental identity salience and strength are related but distinct constructs. We conclude that investigating the nature and effects of environmental identity salience leads to a fruitful path to a more comprehensive understanding of proenvironmental behavior. The proposed new measure may serve as a helpful tool in this endeavor.
Social Media-Based Collaborative Learning Effects on Student Performance/Learner Performance With Moderating Role of Academic Self-Efficacy
Authors: Liu Shuai , Zaigham Ghulam Hussain Khan , Rashid Rao Muhammad , Bilal Ahmad
Published: July 2022

Social media has always been described as the channel through which knowledge is transmitted between communities, students, and learners. This social media has been utilized by university students in a way to encourage collaborative learning and social interaction. This study explores the use of social media in the process of collaborative learning among university students in China using a survey method, a total of 583 students from different universities were surveyed in this study. Through this investigation, different factors enhancing collaborative learning among university students in the context of using social media are going to be examined. Structural equation modeling (SEM) and hierarchical regression were used to analyze the suggested hypothesis. Results show that perceived benefit, active learning, and interaction with students are significantly related to social media collaboration. However, perceived ease of use and perceived usefulness have an insignificant effect on social media collaborative learning. Additionally, students’ academic self-efficacy significantly moderates the relationship between social media collaboration and learning performance. The implication and limitations of the study are also discussed in the last section.
Exploring the Necessity of Psychological Rounds and Psychological Interventions in General Hospitals in the Post-COVID-19 Period
Authors: Tang PeiXi , Lee MaoSheng
Published: July 2022

Objective

To explore the necessity of psychological rounds and psychological intervention in the post-COVID-19 period in a general hospital.

Methods

Based on the current pandemic influence on Chinese people’s psychology, the medical experience, and environment were analyzed, and the feasibility of psychological evaluation and intervention were appraised with the psychological changes that might be brought by the medical behaviors, especially for surgical operations.

Results

Nowadays, the pandemic is under full control in China, although the pandemic is rampant abroad. In China, the “Normalized pandemic prevention” phase has begun. In the post-COVID-19 period, the prolonged pandemic has made numerous people pessimistic, angry, and other negative emotions. Several general hospitals are facing huge influences: under the influence of anxiety, such as “higher hospital-acquired infection rate,” the patient attendance rate is reduced, and the hospital income is sharply reduced. Doctor–patient conflicts are more likely to occur during the medical procedures, affecting the medical experience, and reducing the rate of re-visit and referral.

Conclusion

After analyzing a series of “endogenous” and “exogenous” factors of medical procedures in a general hospital in the post-pandemic period, it suggests that anxiety and depression caused by uncertainties in the medical procedures may be more obvious. Also, it is necessary to pay attention to the psychological status of patients and carry out psychological rounds and psychological interventions in general hospitals. The service quality can be improved, the medical experience can be ameliorated, and it can help general hospitals to turn “crisis” into “opportunity,” which also brings better development.
Should I Stay or Should I Go? Risk Perception and Use of Local Public Transport During the COVID-19 Pandemic
Authors: Helfers Anna , Reiserer Marissa , Schneider Natalie , Ebersbach Mirjam , Sommer Carsten
Published: July 2022

In light of the climate crisis, the transport sector needs to be urgently transformed and the number of users of local public transport needs to be increased. However, the first year of the COVID-19 pandemic severely affected public transport with passenger numbers declining up to 80% in Germany. In addition to a general decrease in mobility during lockdowns, we can observe a shift in decision-making in regards to modes of transportation, with public transport losing out. We argue that this change in behavior can be explained by the fact that people tend to overestimate the risk of COVID-19 transmission in public transport. In order to understand risk perception in users and non-users of public transport during the pandemic, a representative survey (N = 918) in a German major city was conducted at the peak of the third wave of the pandemic in April 2021. We identified four main target groups of public transport use during the pandemic: Loyal users (n = 193), reducers (n = 175), pandemic-dropouts (n = 331) and non-users (n = 219). We found reducers (r = 0.12), pandemic-dropouts (r = 0.32) and non-users (r = 0.22) to perceive an increased perception of infection risk for public transport as compared loyal users. This increased risk perception was specific to public transport – it did not generalize to other day-to-day situations, such as going to the grocery store or visiting a hairdresser. This finding can be taken as an indication that risk perception for an infection plays a crucial role in stepping back from public transport use during the pandemic. In addition, however, there were other differences in terms of needs and concerns between the different target groups during the pandemic. Based on our findings, we discuss which tools and interventions might convince these different groups to hop-(back)-on public transport. Our study highlights how risk perception will play an important role in attracting new and former passengers and is the basis for the interventions and developments that will build a pandemic-resistant public transport in the future.
Efficacy and Safety of Intranasal Esketamine in Patients With Treatment-Resistant Depression and Comorbid Chronic Post-traumatic Stress Disorder: Open-Label Single-Arm Pilot Study
Authors: Rothärmel Maud , Benosman Cherifa , El-Hage Wissam , Berjamin Caroline , Ribayrol Diane , Guillin Olivier , Gaillard Raphaël , Berkovitch Lucie , Moulier Virginie
Published: July 2022

Introduction

Major depressive disorder (MDD) is more likely to resist to usual treatment when it is associated with post-traumatic stress disorder (PTSD). Capitalizing on the effect of ketamine in both treatment-resistant depression (TRD) and PTSD, we conducted a study in order to assess the efficacy of intranasal (IN) Esketamine in patients having TRD with comorbid PTSD.

Materials and Methods

In this open-label, single arm, retrospective pilot study, 11 patients were treated with IN Esketamine (56 or 84 mg) with a longitudinal follow-up of 6 months. IN Esketamine was administered twice weekly during the first month, once weekly during the second month, and then once every 1 or 2 weeks. Patients were assessed with Montgomery-Åsberg Depression Rating Scale (MADRS), Patient Health Questionnaire 9 items, Global Assessment of Functioning (GAF), and Clinical Global Impression-Suicide Scale (CGI-SS).

Results

We included 9 women and 2 men (mean age 47.3 ± 11.1 years). The mean (SD) MADRS scores decreased significantly from 38.6 (6.4) at baseline to 18.2 (10.03) after 6 months of IN Esketamine; 7 patients were responders and 3 patients were in remission. The percentage of patients who were moderately to severely suicidal declined from 63.6% at baseline to 27.3% after 1 month of IN Esketamine sessions. No serious adverse reactions were observed.

Conclusion

This study reports the outcomes of 11 severely ill patients with comorbid TRD and PTSD after IN Esketamine treatment. Esketamine significantly improved depression symptoms, suggesting that it is likely to be a treatment of choice in this specific population.
A Longitudinal Investigation of the Causal Relationship Between Wellbeing and Perceived Discrimination Among Migrant Children in China: The Mediating Role of Self-Esteem and the Moderating Role of School Type
Authors: Wang Qing , Yu Jie , Tang Yuanmeng , Luo Jing , Shi Baoguo
Published: July 2022

Background

A large rural labor force has been attracted to urban areas with the acceleration of urbanization in China. This significant change in environment for migrant children from rural to urban may lead to psychological problems, such as decreased subjective wellbeing (WB) and increased perceived discrimination (PD). However, previous studies have focused on the influence of PD on WB by using a cross-sectional design, ignoring the causality and intrinsic mechanisms between WB and PD. The current study investigates the causal association and internal relations between migrant children’s PD and WB.

Methods

A total of 466 (222 females, 47.64%) migrant children (Mage = 11.78, SD = 1.80) were recruited from Beijing in China. The participants filled in the questionnaire twice, with an interval of 1 year, including a basic information questionnaire, wellbeing index scale, perceived discrimination questionnaire, and self-esteem scale.

Results

Overall, cross-lagged regression analysis revealed that WB (T1) had a predictive effect on PD (T2) but that PD (T1) had no predictive effect on WB (T2). Mediation results indicated that self-esteem (SE) (T1) mediated the relation between WB (T1) and PD (T2). Moderated mediation results further proved that the link between WB (T1) on SE (T1) and the indirect effect between WB (T1) and PD (T2) were more robust for migrant children who attended public school than those in the migrant children’s school.

Conclusion

These findings implied that a decrease in WB may increase the perception of subjective discrimination and that SE could be an intrinsic factor between migrant children’s WB and PD, especially in public schools. Therefore, educators and parents should also pay attention to mental health problems to improve the wellbeing and self-esteem of migrant children.
Genetic Pathways Associated With Sleep Problems in Children With Autism Spectrum Disorder
Authors: Lin Ping-I , Masi Anne , Moni Mohammad Ali , Kummerfeld Sarah , Eapen Valsamma
Published: July 2022

Aims

Children on the autism spectrum are more likely to have sleep problems than non-autistic children. Sleep disturbance may exacerbate emotional and behavioral problems of children on the autism spectrum. A better understanding of the biological mechanisms underlying sleep disturbance provide clues to better management for this co-morbid condition in autism. The goal of the current study is to identify genetic variants associated with sleep disturbance and melatonin levels in autistic children.

Methods

A total of 969 children on the autism spectrum were genotyped using the Global Screening Array v1 or Global Screening Array v2. Sleep problems were assessed using the Children's Sleep Habits Questionnaire (CSHQ). Melatonin levels were measured using the urine samples of 219 probands. The relationship between the melatonin level and CSHQ score was examined using the general linear model. The genetic variants associated with the CSHQ score and melatonin level as two separate quantitative traits were determined using genomewide association studies.

Results

The data indicates that urine melatonin levels were positively associated with CSHQ scores, suggesting that autistic children with a poorer sleep qualiy could has higher melatonin level. Furthermore, genetic assocication studies suggest that genetic pathways involved in pro-inflammatory responses might be involved in sleep disturbance, while genetic pathways involved in catecholamine-secreting PC12 cells and Schwann cells could be associated with melatonin levels.

Conclusions

Taken together, our findings indicate that sleep disturbance and melatonin metabolism could be attributable to distinct biological mechanisms in autistic children since they might not share genetic contributors.
Relationship Between Person-Organization Fit and Teacher Burnout in Kindergarten: The Mediating Role of Job Satisfaction
Authors: Zang Lingling , Chen Yameng
Published: July 2022

As an important organizational factor, person-organization fit in kindergartens may lead to teacher burnout when it is unfitted. In order to explore the influence mechanism of person-organization fit on teacher burnout in kindergartens, this study introduced the variable of job satisfaction to study the mediating effect of job satisfaction on the relationship between person-organization fit and teacher burnout in kindergartens. A total of 637 teachers from Henan, China, were surveyed by Person-Organization Fit Scale, Job Satisfaction Scale and Maslach Burnout Inventory. Results showed that person-organization fit, job satisfaction and teacher burnout were negatively correlated. Person-organization fit is positively correlated with job satisfaction. Job satisfaction partially mediated the relationship between person-organization fit and teacher burnout in kindergartens. In the end, the results of the relationship between person-organization fit, job satisfaction, and teacher burnout in Chinese kindergartens were discussed in this study.
Comparative Analysis of the Wounded in Patients and Deaths in a Hospital Following the Three Major Earthquakes in Western China
Authors: Xu Shan , Shi Bo , Yuxian Jianbo , He Mei , Yang Pei , Xu Weiyun , Liu Gang , Song Zhongjin , Du Xiaobo , Wang Dong
Published: July 2022

The purpose of this study was to analyze the injury characteristics of patients and therapeutic strategies for patients injured in the last three big earthquakes in China, so as to provide a reference for the improvement of emergency plans for earthquakes. The analysis was based on the data provided by the Mianyang Central Hospital (MCH) from May 12th, 2008 to September 26th, 2017. Microsoft EXCEL software was used for data input, and SPSS was used for statistical analysis. A total of 1,390 earthquake-related patients were hospitalized in MCH. Most patients were admitted to the hospital within the first 2 weeks after the earthquake. The main causes for seismic injuries involved hit/strike by objects or building collapse /burying. Extremity fractures accounted for most injuries, especially 3 days after an earthquake. But soft tissue injuries cannot be neglected. Most earthquake patients were mainly treated by means of surgery and the majority were related to orthopedics. We found that different areas, population, and religions needed a tailored approach to the rescue effort. Therefore, the earthquake magnitude scale has a significant influence on mechanisms, types and severity of the injury of patients injured in earthquakes, as well as their timely transfer, management, and prognosis. Traumatic injuries are very common and thereby various surgical procedures especially orthopedic and neurosurgery are the domain of treatment modalities. Disaster preparedness and combined surgical team effort need to be focused on to reduce both mortality and morbidity.
Risky Drinking Cultures Among Affluent Youth in Sweden
Authors: Hiltunen Linda , Kvillemo Pia , Demetry Youstina , Gripenberg Johanna , Elgán Tobias H. , Skoglund Charlotte
Published: July 2022

There is a growing scientific interest in drinking behavior among young people in affluent areas, who report higher levels of alcohol consumption compared to youth in less privileged areas. This phenomenon has been observed in several Western countries. The research has been dominated by variable-oriented analyses and has presented interesting explanations, but there has been little research into these young people's own experiences of and attitudes toward alcohol consumption. To develop interventions targeting this group, we need to understand their lifeworld. This study aims to develop an in-depth understanding of the high alcohol consumption among young people in affluent areas and how they themselves experience it. In the spring of 2019, we conducted 20 in-depth interviews with adolescents in upper secondary school (aged 15–19) in one of the most affluent area in Sweden. The empirical material was analyzed thematically. Theoretically, the phenomenon is understood by relating to social identity processes and considering the group's material, social and cultural means through Bourdieu's metaphors of capital. We found that affluent youth link their social identities to alcohol consumption. Alcohol is a social beverage that opens social networks and contributes to a sense of community. The consumption of alcohol gives experience capital leading to status in this context, with clear norms and expectations governing alcohol consumption. Parties are arranged in protected spaces where young people are free to drink out of the adults' sight. Affluent youths also have considerable purchasing power which contributes to drinking, and they are socialized into a pre-existing adult alcohol culture characterized by a liberal view on alcohol. Finally, when alcohol consumption escalates, the youths perceive that it is difficult to get adequate help from the adult world. The findings are important for future preventive interventions for subgroups of adolescents at high risk for heavy drinking.
An Inverted Container in Containing and Not Containing Hospitalized Patients—A Multidisciplinary Narrative Inquiry
Authors: Gabay Gillie , Ben-Asher Smadar
Published: July 2022

Objective

Patient-centered care calls to contain patients in their time of crisis. This study extends the knowledge of provider patient interactions in the hectic environment of acute care applying Bion's container-contained framework from psychoanalysis.

Methods

Following ethical approval, we performed a narrative inquiry of the experiences of ten patients upon discharge from lengthy hospitalizations in acute care. Interviews were conducted upon discharge and about one-month post-discharge.

Findings

Data analysis suggests four modes of containing of patients by providers. In nurturing interactions, typical of an active container-contained mode, patients experienced humanized care, symptom control, hope, and internal locus of control. This mode yielded patient gratitude toward providers, wellbeing, and post-discharge self-management of diseases. In rigid and wall-free modes of containing, patients experienced a sense of powerlessness and discomfort. A new mode of container-contained was identified, the “Inverted Container”, which extends Bion's theory and contradicts patient-centered care. In inverted containers, patients contained the providers yet reported feeling gratitude toward providers. The gratitude constitutes a defense mechanism and reflects a traumatic experience during hospitalization, which led to post-discharge distrust in providers and hospitals and poor self-management of illness.

Conclusions

To effectively provide patient-centered care, provider-patient interaction in lengthy hospitalizations must move along a clinical axis and a relationship axis. This shifting may facilitate containing patients in their time of crisis so essential processes of reflection, projection, and transference are facilitated in-hospital care.
The Development of a Collaborative Binational Strategy to Support the San Diego-Tijuana Transborder Community During the COVID-19 Pandemic
Authors: Jiménez Barbara , Kozo Justine
Published: July 2022

The COVID-19 pandemic illustrates the need for and importance of cross-border public health collaboration. San Diego, California and Tijuana, Baja California are an interconnected region with one of the busiest international borders in the world and hundreds of thousands bi-directional crossings each day. As the sister cities witnessed the rising case numbers early in the pandemic, it became essential and urgent to implement a formal structure to facilitate cross-border COVID-19 communication, coordination, and collaboration. The present article describes how the development of a Collaborative Binational Strategy led to coordinated outreach and initiatives that addressed access and equity in the transborder region. Through examples, the article illustrates how regional leaders in San Diego and Tijuana harnessed existing transborder partnerships to collaboratively build infrastructure and communication pathways to exchange data, guidance, troubleshoot shared challenges, build capacity, and establish cross-border testing and vaccine opportunities. The challenges, lessons learned, and best practices may inform other multi-level, interdisciplinary, and cross-border jurisdictions on how to support a transborder community during a pandemic or other health emergency.
Factors Related to Sexual Intercourse Among Korean Middle and High School Students
Authors: Shim Joohee , Baek Jihyun , Han Seungwoo
Published: July 2022

Background

Social interest in sexual intercourse among teenagers is increasing in Korea. This study aimed to identify factors related to sexual intercourse among adolescents.

Methods

This is a secondary analysis study using data from the 2020 Korea Youth Risk Behavior Survey, with information regarding 54,948 middle school and high school students in Korea having been analyzed. Demographics, health behavior, and mental health characteristics were included. Data evaluation involved chi-square test and binary multivariable logistic regression analysis.

Results

For middle school students, sexual intercourse was related to their housing type [Odds Ratio (OR) = 10.698], smoking (OR = 5.165) and drinking (OR = 2.229) experience, suicide attempt (OR = 1.920), gender (OR = 1.632), loneliness (OR = 1.501), sadness and despair (OR = 1.493), year level (OR = 1.457), happiness (OR = 0.772), sleep status (OR = 0.716), economic status (OR = 0.705). For high school students, sexual intercourse was related to their smoking experience (OR = 4.746), housing type (OR = 3.661), drinking experience (OR = 2.840), drug use (OR = 2.511), suicide attempt (OR = 2.071), year level (OR = 1.989), sadness and despair (OR = 1.506), gender (OR = 1.429), suicide plan (OR = 1.402), loneliness (OR = 1.270), academic performance (OR = 1.165), happiness (OR = 0.865), subjective health status (OR = 0.814), and economic status (OR = 0.727). However, sleep status was statistically significant for middle school students, but not for high school students. Academic performance, drug use, suicide plan, and subjective health status were statistically significant in high school students, but not in middle school students.

Conclusion

A program that can directly mediate these factors must be prepared. When conducting sex education for adolescents, mental health-related factors must also be considered.
Difference in the Sexual and Reproductive Health of Only-Child Students and Students With Siblings, According to Sex and Region: Findings From the National College Student Survey
Authors: Zhao Shuangyu , Liang Yun , Hee Jia Yi , Qi Xinran , Tang Kun
Published: July 2022

Objective

The differences in sexual knowledge, attitudes, behaviors, seeking behaviors for sex-related knowledge, and sexual and reproductive health (SRH) outcomes among only-child students and students with siblings in China, was examined for sex- and region- specific effects.

Research Design and Methods

Data on 49,569 students from the 2019 National College Student Survey on Sexual and Reproductive Health, conducted across 31 provinces in mainland China was utilized. Multivariable regression and stratified analyses were employed to analyze the differences in sexual and reproductive health between only-child students and students with siblings.

Results

Only-child students reported higher sexual knowledge, more liberal sexual attitudes, and fewer adverse SRH outcomes compared to those with siblings. Results were found to be influenced by sex and hometown region after controlling for socio-economic factors, parent-child relationship, and sexuality education.

Conclusions

Female students with siblings who resided in rural regions were more likely to have poorer SRH compared to male only-child students who resided in urban regions. Comprehensive sexual education for students should aim to better include females and students from rural areas both offline and online, and public healthcare should offer subsidized consultations and contraceptives.
“What Other Information Is There?”: Identifying Information Gaps, Perceptions and Misconceptions on COVID-19 Among Minority Ethnic Groups in the Netherlands
Authors: Bakuri Amisah Zenabu , Antwi-Berko Daniel
Published: July 2022

Background

Multiple media platforms and various resources are available for information on the novel coronavirus disease (COVID-19). Identifying people's preferences is key to building public confidence and planning for successful national or regional health intervention strategies.

Methods

Using exploratory mixed-methods including a short survey, interviews and participant observation, this cross-sectional study of 160 respondents from the Ghanaian-Dutch, Afro and Hindustani Surinamese-Dutch communities in Amsterdam, the Netherlands was conducted. Data collected between February to April 2021, included demographics characteristics, knowledge, opinions, preferred source of information, behavioral factors, and information gaps on COVID-19 prevention measures, responses and decision-making of respondents. Descriptive statistics and follow-up in-depth interviews were conducted to determine the relationship between respondents' demographics, information sources, and attitudes/behaviors toward COVID-19.

Results

The findings of this study indicated that although many of the respondents from these communities had good knowledge on COVID-19, its modes of transmission and prevention measures, their willingness to take up initiatives and prioritize self responsibility toward their health are tied to their communal life. The respondents in this study demonstrated high value for social lives and relied on their connections with friends and families in shaping, obtaining, processing and utilizing COVID-19 information to build a sense of responsibility toward the uptake of COVID-19 prevention measures despite recent decline in number of cases.

Conclusion

This sense of responsibility means their active participation and ownership of interventions to address the specific personal concerns and that of their community. However, different factors play influential roles toward the behavior choices of our respondents regarding the COVID-19 prevention.