bluuebunny/crossref_metadata_2025 huggingface dataset:
Case Report: Use of Anakinra in Multisystem Inflammatory Syndrome During COVID-19 Pandemic
Authors: Della Paolera Sara , Valencic Erica , Piscianz Elisa , Moressa Valentina , Tommasini Alberto , Sagredini Raffaella , Kiren Valentina , Comar Manola , Taddio Andrea
Published: February 2021

During COVID-19 outbreak, a large number of children with severe inflammatory disease has been reported. This condition, named Pediatric Multi-inflammatory Syndrome temporally associated with COVID-19 (PIMS-TS) or Multisystem Inflammatory Syndrome associated with Coronavirus Disease 2019 (MIS-C), shares some clinical features with Kawasaki disease and is frequently complicated by myocarditis or shock. It has been suggested that MIS-C belongs to the group of cytokine storm syndromes triggered by SARS-CoV-2 infection. So far, intravenous immunoglobulin (IVIG) and systemic glucocorticoids are the most common therapeutic approaches reported in this group of patients. However, the use of anakinra in patients with severe forms of COVID-19 is showing promising results. Here we reported two patients with multisystem inflammatory syndrome complicated with shock. Both the patients presented a poor response to IVIG and systemic glucocorticoids and received anakinra. Treatment with IL-1 receptor antagonist showed a rapid improvement of clinical conditions and biochemical analysis in both patients and demonstrated a good safety profile. Thus, we look forward for future controlled clinical trials with the aim to demonstrate the effectiveness of anakinra in patients with MIS-C and established precise criteria for its use.
Effect and Mechanism of Catalpol on Remyelination via Regulation of the NOTCH1 Signaling Pathway
Authors: Sun Yaqin , Ji Jing , Zha Zheng , Zhao Hui , Xue Bing , Jin Liangyun , Wang Lei
Published: February 2021

Promoting the differentiation of oligodendrocyte precursor cells (OPCs) is important for fostering remyelination in multiple sclerosis. Catalpol has the potential to promote remyelination and exert neuroprotective effects, but its specific mechanism is still unclear. Recent studies have shown that the NOTCH1 signaling pathway is involved in mediating OPC proliferation and differentiation. In this study, we elucidated that catalpol promoted OPC differentiationin vivoand vitro and explored the regulatory role of catalpol in specific biomolecular processes. Following catalpol administration, better and faster recovery of body weight and motor balance was observed in mice with cuprizone (CPZ)-induced demyelination. Luxol fast blue staining (LFB) and transmission electron microscopy (TEM) showed that catalpol increased the myelinated area and improved myelin ultrastructure in the corpus callosum in demyelinated mice. In addition, catalpol enhanced the expression of CNPase and MBP, indicating that it increased OPC differentiation. Additionally, catalpol downregulated the expression of NOTCH1 signaling pathway-related molecules, such as JAGGED1, NOTCH1, NICD1, RBPJ, HES5, and HES1. We further demonstrated thatin vitro, catalpol enhanced the differentiation of OPCs into OLs and inhibited NOTCH1 signaling pathway activity. Our data suggested that catalpol may promote OPC differentiation and remyelination through modulation of the NOTCH1 pathway. This study provides new insight into the mechanism of action of catalpol in the treatment of multiple sclerosis.
Science in Underground Laboratories and DULIA-Bio
Authors: Ianni Aldo
Published: February 2021

This paper reports a brief introduction to Deep Underground Laboratories (DULs) and the connection they have with research on biology in extreme environments and the effect of radiation in life. Presently, there are 14 DULs in operation worldwide. Although the main research activity in these infrastructures concerns the search for rare events in astroparticle physics and neutrino physics, DULs offer a unique opportunity to undertake experiments in astrobiology and biology in extreme environments. This is the main motivation of Deep Underground Laboratory Integrated Activity in biology (DULIA-bio) 2019 Workshop, which was held at the Gran Sasso (Italy) underground laboratory. This paper aims to give an introduction to the subject of the Workshop by reviewing the main features of DULs.
Epigenetics, 1-Carbon Metabolism, and Homocysteine During Dysbiosis
Authors: Singh Mahavir , Hardin Shanna J. , George Akash K. , Eyob Wintana , Stanisic Dragana , Pushpakumar Sathnur , Tyagi Suresh C.
Published: February 2021

Although a high-fat diet (HFD) induces gut dysbiosis and cardiovascular system remodeling, the precise mechanism is unclear. We hypothesize that HFD instigates dysbiosis and cardiac muscle remodeling by inducing matrix metalloproteinases (MMPs), which leads to an increase in white adipose tissue, and treatment with lactobacillus (a ketone body donor from lactate; the substrate for the mitochondria) reverses dysbiosis-induced cardiac injury, in part, by increasing lipolysis (PGC-1α, and UCP1) and adipose tissue browning and decreasing lipogenesis. To test this hypothesis, we used wild type (WT) mice fed with HFD for 16 weeks with/without a probiotic (PB) in water. Cardiac injury was measured by CKMB activity which was found to be robust in HFD-fed mice. Interestingly, CKMB activity was normalized post PB treatment. Levels of free fatty acids (FFAs) and methylation were increased but butyrate was decreased in HFD mice, suggesting an epigenetically governed 1-carbon metabolism along with dysbiosis. Levels of PGC-1α and UCP1 were measured by Western blot analysis, and MMP activity was scored via zymography. Collagen histology was also performed. Contraction of the isolated myocytes was measured employing the ion-optic system, and functions of the heart were estimated by echocardiography. Our results suggest that mice on HFD gained weight and exhibited an increase in blood pressure. These effects were normalized by PB. Levels of fibrosis and MMP-2 activity were robust in HFD mice, and treatment with PB mitigated the fibrosis. Myocyte calcium-dependent contraction was disrupted by HFD, and treatment with PB could restore its function. We conclude that HFD induces dysbiosis, and treatment with PB creates eubiosis and browning of the adipose tissue.
Correlation of Coagulation Parameters With Clinical Outcomes During the Coronavirus-19 Surge in New York: Observational Cohort
Authors: Reyes Gil Morayma , Gonzalez-Lugo Jesus D. , Rahman Shafia , Barouqa Mohammad , Szymanski James , Ikemura Kenji , Lo Yungtai , Billett Henny H.
Published: February 2021

Importance

COVID-19 has caused a worldwide illness and New York became the epicenter of COVID-19 in the United States from Mid-March to May 2020.

Objective

To investigate the coagulopathic presentation of COVID and its natural course during the early stages of the COVID-19 surge in New York. To investigate whether hematologic and coagulation parameters can be used to assess illness severity and death.

Design

Retrospective case study of positive COVID inpatients between March 20, 2020-March 31, 2020.

Setting

Montefiore Health System main hospital, Moses, a large tertiary care center in the Bronx.

Participants

Adult inpatients with positive COVID tests hospitalized at MHS.

Exposure (for observational studies)

Datasets of participants were queried for demographic (age, sex, socioeconomic status, and self-reported race and/or ethnicity), clinical and laboratory data.

Main Outcome and Measures

Relationship and predictive value of measured parameters to mortality and illness severity.

Results

Of the 225 in this case review, 75 died during hospitalization while 150 were discharged home. Only the admission PT, absolute neutrophil count (ANC) and first D-Dimer could significantly differentiate those who were discharged alive and those who died. Logistic regression analysis shows increased odds ratio for mortality by first D-Dimer within 48 hrs. of admission. The optimal cut-point for the initial D-Dimer to predict mortality was found to be 2.1 μg/mL. 15% of discharged patients required readmission and more than a third of readmitted patients died (5% of all initially discharged).

Conclusion

We describe here a comprehensive assessment of hematologic and coagulation parameters in COVID-19 and examine the relationship of these to mortality. We demonstrate that both initial and maximum D-Dimer values are biomarkers that can be used for survival assessments. Furthermore, D-Dimer may be useful to follow up discharged patients.
AKH Signaling in D. melanogaster Alters Larval Development in a Nutrient-Dependent Manner That Influences Adult Metabolism
Authors: Hughson Bryon N. , Shimell MaryJane , O’Connor Michael B.
Published: February 2021

Metabolism, growth, and development are intrinsically linked, and their coordination is dependent upon inter-organ communication mediated by anabolic, catabolic, and steroid hormones. InDrosophila melanogaster, the corpora cardiaca (CC) influences metabolic homeostasis through adipokinetic hormone (AKH) signaling. AKH has glucagon-like properties and is evolutionarily conserved in mammals as the gonadotropin-releasing hormone, but its role in insect development is unknown. Here we report that AKH signaling alters larval development in a nutrient stress-dependent manner. This activity is regulated by the locusdg2, which encodes a cGMP-dependent protein kinase (PKG). CC-specific downregulation ofdg2expression delayed the developmental transition from larval to pupal life, and altered adult metabolism and behavior. These developmental effects were AKH-dependent, and were observed only in flies that experienced low nutrient stress during larval development. Calcium-mediated vesicle exocytosis regulates ecdysteroid secretion from the prothoracic gland (PG), and we found that AKH signaling increased cytosolic free calcium levels in the PG. We identified a novel pathway through which PKG acts in the CC to communicate metabolic information to the PG via AKH signaling. AKH signaling provides a means whereby larval nutrient stress can alter developmental trajectories into adulthood.
Variable Gut Function Recovery After Right vs. Left Colectomy May Be Due to Rectosigmoid Hyperactivity
Authors: Seo Sean Ho Beom , Bissett Ian , O’Grady Gregory
Published: February 2021

It is established that gut function recovers slower after right vs. left colectomies with higher rates of prolonged post-operative ileus (PPOI), but the reason is unclear. Development of PPOI is multifactorial. A recent manometry study in right colectomy patients showed that the distal colon becomes hyperactive after surgery with predominantly cyclic motor patterns (CMPs). In this perspective, we evaluate the hypothesis that the slower gut recovery after right hemicolectomy could be induced by a functional obstruction due to hyperactive CMPs.
A Centrifuge-Based Method for Identifying Novel Genetic Traits That Affect Root-Substrate Adhesion in Arabidopsis thaliana
Authors: Eldridge Bethany M. , Larson Emily R. , Weldon Laura , Smyth Kevin M. , Sellin Annabelle N. , Chenchiah Isaac V. , Liverpool Tanniemola B. , Grierson Claire S.
Published: February 2021

The physical presence of roots and the compounds they release affect the cohesion between roots and their environment. However, the plant traits that are important for these interactions are unknown and most methods that quantify the contributions of these traits are time-intensive and require specialist equipment and complex substrates. Our lab developed an inexpensive, high-throughput phenotyping assay that quantifies root-substrate adhesion in Arabidopsis thaliana. We now report that this method has high sensitivity and versatility for identifying different types of traits affecting root-substrate adhesion including root hair morphology, vesicle trafficking pathways, and root exudate composition. We describe a practical protocol for conducting this assay and introduce its use in a forward genetic screen to identify novel genes affecting root-substrate interactions. This assay is a powerful tool for identifying and quantifying genetic contributions to cohesion between roots and their environment.
The Cytokinin Status of the Epidermis Regulates Aspects of Vegetative and Reproductive Development in Arabidopsis thaliana
Authors: Werner Sören , Bartrina Isabel , Novák Ondřej , Strnad Miroslav , Werner Tomáš , Schmülling Thomas
Published: February 2021

The epidermal cell layer of plants has important functions in regulating plant growth and development. We have studied the impact of an altered epidermal cytokinin metabolism onArabidopsisshoot development. Increased epidermal cytokinin synthesis or breakdown was achieved through expression of the cytokinin synthesis geneLOG4and the cytokinin-degradingCKX1gene, respectively, under the control of the epidermis-specificAtML1promoter. During vegetative growth, increased epidermal cytokinin production caused an increased size of the shoot apical meristem and promoted earlier flowering. Leaves became larger and the shoots showed an earlier juvenile-to-adult transition. An increased cytokinin breakdown had the opposite effect on these phenotypic traits indicating that epidermal cytokinin metabolism can be a factor regulating these aspects of shoot development. The phenotypic consequences of abbreviated cytokinin signaling in the epidermis achieved through expression of the ARR1-SRDX repressor were generally milder or even absent indicating that the epidermal cytokinin acts, at least in part, cell non-autonomously. Enhanced epidermal cytokinin synthesis delayed cell differentiation during leaf development leading to an increased cell proliferation and leaf growth. Genetic analysis showed that this cytokinin activity was mediated mainly by the AHK3 receptor and the transcription factor ARR1. We also demonstrate that epidermal cytokinin promotes leaf growth in a largely cell-autonomous fashion. Increased cytokinin synthesis in the outer layer of reproductive tissues and in the placenta enhanced ovule formation by the placenta and caused the formation of larger siliques. This led to a higher number of seeds in larger pods resulting in an increased seed yield per plant. Collectively, the results provide evidence that the cytokinin metabolism in the epidermis is a relevant parameter determining vegetative and reproductive plant growth and development.
Spatial Language of Young Children During Block Play in Kindergartens in Urban China
Authors: Yang Xiaoli , Pan Yuejuan
Published: February 2021

Spatial language is an important predictor of spatial skills and might be inspired by peer interaction and goal-oriented building behaviors during block play. The present study investigated the frequency, type and level of children’s spatial language during block play and their associations with the level of block play by observing 228 young children in classrooms equipped with unit blocks and allowing free play on a daily basis. The findings showed that during block play, young children used more words about spatial locations, deictic terms, dimensions, and shapes and fewer words about spatial features or properties and spatial orientations or transformations. Spatial locations were used most frequently, and young children tended to use vertical location words to represent the corresponding location. Most young children used gestures in conjunction with spatial deictic terms. Among shape words, tetragon words were frequently used, and the representation of spatial shapes showed alternatives, collective tendencies and gender differences. The use of spatial language during the play process had a significant positive correlation with age, the construction structure, and form of block building.
Behavioral and ERP Correlates of Long-Term Physical and Mental Training on a Demanding Switch Task
Authors: Burgos Pablo I. , Cruz Gabriela , Hawkes Teresa , Rojas-Sepúlveda Ignacia , Woollacott Marjorie
Published: February 2021

Physical and mental training are associated with positive effects on executive functions throughout the lifespan. However, evidence of the benefits of combined physical and mental regimes over a sedentary lifestyle remain sparse. The goal of this study was to investigate potential mechanisms, from a source-resolved event-related-potential perspective, that could explain how practicing long-term physical and mental exercise can benefit neural processing during the execution of an attention switching task. Fifty-three healthy community volunteers who self-reported long-term practice of Tai Chi (n = 10), meditation + exercise (n = 16), simple aerobics (n = 15), or a sedentary lifestyle (n = 12), aged 47.8 ± 14.6 (SD) were included in this analysis. All participants undertook high-density electroencephalography recording during a switch paradigm. Our results indicate that people who practice physical and mental exercise perform better in a task-switching paradigm. Our analysis revealed an additive effect of the combined practice of physical and mental exercise over physical exercise only. In addition, we confirmed the participation of frontal, parietal and cingulate areas as generators of event-related-potential components (N2-like and P3-like) commonly associated to the performance of switch tasks. Particularly, the N2-like component of the parietal and frontal domains showed significantly greater amplitudes in the exercise and mental training groups compared with aerobics and sedentary groups. Furthermore, we showed better performance associated with greater N2-like amplitudes. Our multivariate analysis revealed that activity type was the most relevant factor to explain the difference between groups, with an important influence of age, and body mass index, and with small effects of educational years, cardiovascular capacity, and sex. These results suggest that chronic combined physical and mental training may confer significant benefits to executive function in normally aging adults, probably through more efficient early attentional processing. Future experimental studies are needed to confirm our results and understand the mechanisms on parieto-frontal networks that contribute to the cognitive improvement associated with practicing combined mental and aerobic exercise, while carefully controlling confounding factors, such as age and body mass index.
Self-Perception of Changes in Routines in Adults and Older Adults Associated to Social Distancing Due to COVID-19—A Study in São Paulo, Brazil
Authors: Machado-Lima Adriana , Alonso Angélica Castilho , Gozzo Débora , Zanca Gisele Garcia , Brech Guilherme Carlos , Montiel José Maria , Bastos Marta Ferreira , Longo Priscila Larcher , Mota-Ortiz Sandra Regina
Published: February 2021

COVID-19 is an acute respiratory illness with higher mortality in older adults. This condition is spread person-to-person through close contact, and among policies employed to decrease transmission are the improvement of hygiene habits and physical distancing. Although social distancing has been recognized as the best way to prevent the transmission, there are concerns that it may promote increased depression symptoms risk and anxiety, mainly in older adults. This cross-sectional study aimed to verify self-concept of social distancing in adults compared to older adults. All participants, over 18 years and residents of São Paulo state (Brazil), were invited to join this research study by a message application and answered an interdisciplinary questionnaire during the period from May 23 to June 23, 2020. The questions were divided into the following aspects: sociodemographic data, financial conditions, routine-related perception, perception of health, physical and emotional state, and eating habits. The younger adult group was composed of 139 participants, with a mean age of 43.15 years (±10.92), and the older adult group was composed of 437 participants with a mean age of 67.59 years (±6.13) of both sex. Changes in routine during the period of social distance were reported by 95% of adults and 96.8% of older adults, but adults indicated more significant alterations in routine. Although there was no difference between groups for several aspects, adults revealed greater alterations in sleep quality, evacuation frequency, and more difficulty to perform daily activities at home. Further studies are necessary to follow up the impacts of social distancing among adults and older adults in different socioeconomic contexts to better understand the long-term alterations and the necessity of interventions.
Psychometric Properties of the MSLQ-B for Adult Distance Education in China
Authors: Zhou Ying , Wang Jianhua
Published: February 2021

In the education context, The Motivational Strategies for Learning Questionnaire (MSLQ) is extensively used in assessing self-regulated learning strategies. However, more research is needed to address whether it is applicable for distance education. The exploratory and confirmatory factor analysis were used to test the Chinese version of the Motivated Strategies for Learning Questionnaire part-B for distance learning (MSLQ-B-DL) using two samples totalling 385 participants. This paper substantiates MSLQ-B-DL's criterion-related, convergent, and factorial validity, as well as its internal consistency, in China. Specifically, the concurrent validity of the MSLQ-B-DL was shown from three aspects: (a) the negative correlation of MSLQ-B-DL with trait procrastination; (b) the positive correlation of MSLQ-B-DL with self-control; and (c) the positive correlation of MSLQ-B-DL with instrumental help-seeking and the former's negative correlation with help-seeking avoidance and executive help-seeking. Finally, this study highlights the MSLQ-B-DL's validity and reliability in evaluating the learning strategies in adult distance education in China.
Employee-Athletes: Exploring the Elite Spanish Athletes' Perceptions of Combining Sport and Work
Authors: Moreno Rubén , Chamorro José L. , López de Subijana Cristina
Published: February 2021

Researchers have studied the athletes' dual careers with the aim of helping them to combine the sport and the academic–vocational sphere. Most of this research has addressed the study–sport combination, but there is a lack of studies on the work–sport combination. The main objective of this research was to explore the subjective perceptions of Spanish elite athletes when attempting to combine their careers as professional athletes with a second profession or trade. Further, this study aims to identify the access to facilitating resources and the perception of obstacles and barriers to the development of a dual career. A qualitative approach was chosen to address these research questions. Interpretative phenomenological analysis was carried out on a sample of 18 elite athletes, and the data were collected using semi-structured interviews based on a set of superordinate and subordinate categories. The results showed that the athletes interviewed possessed valuable resources such as the transference of sports values to the work sphere. However, important barriers were highlighted such as the perception of sport institutions as absent entities in the work–sport combination. Thus, the study of this type of dual career seems to be one of the challenges facing the interested scientific community for the coming years.
The Effect of School Psychologists and Social Workers on School Achievement and Failure: A National Multilevel Study in Chile
Authors: López Verónica , Cárdenas Karen , González Luis
Published: February 2021

School achievement and failure have become growing political and social concerns due to the negative consequences of school failure for individuals and society. The inclusive educational movement, which calls for equal access, permanence, participation, and promotion of all students worldwide, poses many challenges for schools and school systems. As a public policy strategy, some countries have provided additional funds for incorporating non-teaching professionals such as school psychologists and social workers in regular K-12 schools. However, there is lack of research on the effects of these psychosocial professionals on student outcomes. This national multilevel study explored the effect of psychologists (n= 8,469) and social workers (n= 3,524) on indicators of eighth-grade (n= 147,531) and 10th-grade (n= 106,347) students' academic achievement and dropout in Chile. A multilevel secondary analysis was performed using national records of non-teaching professionals working as school staff members, achievement scores on the national SIMCE test, and dropout rates based on official records. Results showed that after controlling for individual and school variables known to affect achievement and dropout, schools with psychologists and social workers working as staff members had lower short- and long-term dropout rates. The presence and higher number of school psychologists per school was positively associated with higher math achievement, with a reduced effect in low-SES schools. Lower-SES schools with more social workers had higher math scores. These results support policies that increase funding for school psychologists and social workers, since their incorporation partly explains better school achievement and less school failure when controlling for individual and school characteristics, but emphasize the need to further explore the mechanisms through which school achievement and failure are developed with the support of psychologists and social workers in schools. We discuss the need to regulate the type of prevention and intervention strategies from a whole-school, evidence-based approach, as well as to incorporate psychosocial training modules and comprehensive guidelines as part of professional training programs and as certified requisites for working in schools.
Exploring Computational Thinking Skills Training Through Augmented Reality and AIoT Learning
Authors: Lin Yu-Shan , Chen Shih-Yeh , Tsai Chia-Wei , Lai Ying-Hsun
Published: February 2021

Given the widespread acceptance of computational thinking (CT) in educational systems around the world, primary and higher education has begun thinking about how to cultivate students' CT competences. The artificial intelligence of things (AIoT) combines artificial intelligence (AI) and the Internet of things (IoT) and involves integrating sensing technologies at the lowest level with relevant algorithms in order to solve real-world problems. Thus, it has now become a popular technological application for CT training. In this study, a novel AIoT learning with Augmented Reality (AR) technology was proposed and explored the effect of CT skills. The students used AR applications to understand AIoT applications in practice, attempted the placement of different AR sensors in actual scenarios, and further generalized and designed algorithms. Based on the results of the experimental course, we explored the influence of prior knowledge and usage intention on students' CT competence training. The results show that proposed AIoT learning can increase students' learning intention and that they had a positive impact on problem solving and comprehension with AR technology, as well as application planning and design.
Using Natural Language Processing and Artificial Intelligence to Explore the Nutrition and Sustainability of Recipes and Food
Authors: van Erp Marieke , Reynolds Christian , Maynard Diana , Starke Alain , Ibáñez Martín Rebeca , Andres Frederic , Leite Maria C. A. , Alvarez de Toledo Damien , Schmidt Rivera Ximena , Trattner Christoph , Brewer Steven , Adriano Martins Carla , Kluczkovski Alana , Frankowska Angelina , Bridle Sarah , Levy Renata Bertazzi , Rauber Fernanda , Tereza da Silva Jacqueline , Bosma Ulbe
Published: February 2021

In this paper, we discuss the use of natural language processing and artificial intelligence to analyze nutritional and sustainability aspects of recipes and food. We present the state-of-the-art and some use cases, followed by a discussion of challenges. Our perspective on addressing these is that while they typically have a technical nature, they nevertheless require an interdisciplinary approach combining natural language processing and artificial intelligence with expert domain knowledge to create practical tools and comprehensive analysis for the food domain.
Closed-Loop Control for Fluid Resuscitation: Recent Advances and Future Challenges
Authors: Gholami Behnood , Haddad Wassim M. , Bailey James M. , Muir William W.
Published: February 2021

Fluid therapy is extensively used to treat traumatized patients as well as patients during surgery. The fluid therapy process is complex due to interpatient variability in response to therapy as well as other complicating factors such as comorbidities and general anesthesia. These complexities can result in under- or over-resuscitation. Given the complexity of the fluid management process as well as the increased capabilities in hemodynamic monitoring, closed-loop fluid management can reduce the workload of the overworked clinician while ensuring specific constraints on hemodynamic endpoints are met with higher accuracy. The goal of this paper is to provide an overview of closed-loop control systems for fluid management and highlight several key steps in transitioning such a technology from bench to the bedside.
Value Co-Creation Process and Measurement in 4.0 SMEs: An Exploratory Research in a B2B Marketing Innovation Context
Authors: Royo-Vela Marcelo , Velasquez Serrano Mariell
Published: February 2021

The purpose of this exploratory research is to contribute to the lack of empirical research exploring techniques and protocols that can be used to measure the level of value obtained from using these technologies in the various marketing processes and in a business-to-business (B2B) context. By doing so, firms operating in fast changing dynamic environments can develop the right means to continuously adapt, integrate, reconfigure, and redeploy resources and capabilities to become more competitive and disruptive in their offerings. The phenomenon of interest is described by applying a case study qualitative approach to three 4.0 companies which use virtual reality (VR)/augmented reality (AR) technologies and by carrying out ten in-depth interviews to managers in those organizations. Results show that some small and medium sized enterprises (SMEs) are more rigorous about performance and tracking metrics compared to other companies providing similar technological services. In this line, results show two stages where potential value can be generated and measured when utilizing VR/AR technologies. The first is during testing and development of VR/AR simulations. In this stage, only the ones with strong research and academic background have been developing and using tracking systems, guidelines, and protocols as resources to measure the value obtained from using these technologies in marketing innovation processes. The second moment where value creation can be measured is during the implementation of the VR/AR simulation with the target user. Further research is needed to develop standardization guidelines and protocols that guarantee the success of the simulations delivered to the hiring firms.
Effects of Different Storage Conditions on the Browning Degree, PPO Activity, and Content of Chemical Components in Fresh Lilium Bulbs (Liliumbrownii F.E.Brown var. viridulum Baker.)
Authors: Zhao Kanghong , Xiao Zhengpeng , Zeng Jianguo , Xie Hongqi
Published: February 2021

Although Lilium brownii (L. brownii) bulbs are popular fresh vegetables, a series of quality problems still remain after harvest. In this study, fresh L. brownii bulbs were placed in the dark at 25, 4, and −20 °C and under light at 25 °C from 0 to 30 days; the chemical compositions were analyzed by ultraviolet spectrophotometry (UV) and high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS). During the 30-day storage period, the browning degree increased over the storage time and with increasing temperature, but the contents of proteins and free amino acids decreased and were aggravated by light. The total polyphenol content increased until the 6th day at 25 °C (dark or light), but it did not significantly accumulate at −20 or 4 °C. The reducing sugar content showed a dynamic balance, but the total polysaccharide content decreased constantly in the four storage conditions. The polyphenol oxidase (PPO) activity increased with storage time and increasing temperature, while it was inhibited by light. The increase rates of malondialdehyde (MDA) content at −20 °C and light (25 °C) were higher than those at 4 and 25 °C. In addition, 12 secondary metabolites were identified, most of which accumulated during the storage period, for example, 1-O-feruloyl-3-O-β-D-glucopyranosylglycerol; 1,3-O-di-p-coumaroylglycerol; 1-O-feruloyl-3-O-p-coumaroylglycerol; and 1,2-O-diferuloylglycerol. The variations in nutrient levels had a low correlation with browning, but the variations in MDA, PPO, and secondary metabolite (phenolic acids) levels had a high correlation with browning. In conclusion, fresh L. brownii bulbs should be stored at a low temperature (4 °C) and in dark condition, and browning bulbs are excellent materials for secondary metabolite utilization.
The Effect of Mother Goat Presence during Rearing on Kids’ Response to Isolation and to an Arena Test
Authors: Winblad von Walter Louise , Forkman Björn , Högberg Madeleine , Hydbring-Sandberg Eva
Published: February 2021

The aim of the study was to examine how early permanent separation, separation during the day only, or full-time access to the mother goat affected goat kids during social isolation with a sudden sound of a dog bark at two weeks and two months, and a novel arena test with a novel object at two months. Kids permanently separated reduced their vocalization earlier and had a higher heart rate before and after dog bark during isolation at two weeks, no effect was found on the daytime separated kids. Daytime separated kids bleated more at two weeks and decreased heart rate after dog barking at two months. Daytime separated kids showed the strongest fear reaction in the arena test, no effect was found on the permanently separated kids. Kids separated early vocalized more before novel object and showed more explorative behavior afterwards. Our study shows different responses in goat kids separated early permanent, daytime separated, or kept full-time with mother, which demonstrates the importance of if and how the mother is present, and the impact of using a wide variety of physiological and behavioral measures when evaluating stress in animal welfare research.
Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos
Authors: Hirata Maki , Wittayarat Manita , Namula Zhao , Anh Le Quynh , Lin Qingyi , Takebayashi Koki , Thongkittidilok Chommanart , Tanihara Fuminori , Otoi Takeshige
Published: February 2021

Liposome-mediated gene transfer has become an alternative method for establishing a gene targeting framework, and the production of mutant animals may be feasible even in laboratories without specialized equipment. However, how this system functions in mammalian oocytes and embryos remains unclear. The present study was conducted to clarify whether blastocyst genome editing can be performed by treatment with lipofection reagent, guide RNA, and Cas9 for 5 h without using electroporation or microinjection. A mosaic mutation was observed in blastocysts derived from zona pellucida (ZP)-free oocytes following lipofection treatment, regardless of the target genes. When lipofection treatment was performed after in vitro fertilization (IVF), no significant differences in the mutation rates or mutation efficiency were found between blastocysts derived from embryos treated at 24 and 29 h from the start of IVF. Only blastocysts from embryos exposed to lipofection treatment at 29 h after IVF contained biallelic mutant. Furthermore, there were no significant differences in the mutation rates or mutation efficiency between blastocysts derived from embryos at the 2- and 4-cell stages. This suggests that lipofection-mediated gene editing can be performed in ZP-free oocytes and ZP-free embryos; however, other factors affecting the system efficiency should be further investigated.
Effects of Corn Silage Inclusion Level and Type of Anabolic Implant on Animal Growth Performance, Apparent Total Tract Digestibility, Beef Production per Hectare, and Carcass Characteristics of Finishing Steers
Authors: Buckhaus Elizabeth M. , Smith Zachary K.
Published: February 2021

Maine-Anjou × Angus cross-bred steers (n = 156 steers; initial body weight (BW) 366 ± 37.2 kg) were used in a 132 d finishing study conducted at the Ruminant Nutrition Center (RNC) in Brookings, SD. Steers were blocked by weight (n = 5 BW blocks) and randomly assigned to an implant and dietary treatment of a randomized complete block design with each pen containing seven to eight steers (n = 20 pens). Dietary treatments consisted of (1) 15% (CS15) or (2) 30% corn silage (CS30) where corn silage displaced corn grain in the diet. Steers received one of two implants (both from Zoetis, Parsippany, NJ) containing equal doses of trenbolone acetate (TBA) and estradiol benzoate (EB): (1) Synovex PLUS (non-coated implant; 200 mg TBA and 28 mg EB; PLUS) or (2) Synovex ONE Feedlot (coated implant; 200 mg TBA and 28 mg EB; ONE-F). Bunks were managed using a slick bunk approach, and all diets contained dry matter (DM) basis 33 mg/kg monensin sodium. All steers were offered ad libitum access to feed, and feeding occurred twice daily in equal portions. There was no interaction between the implant and dietary treatment for any variables measured (p ≥ 0.08). Carcass-adjusted basis final BW, average daily gain (ADG), and grain to feed (G:F) were increased (p ≤ 0.02) by 2.2%, 6.5%, and 7.2%, respectively, for CS15. Observed net energy (NE) and the ratio of observed-to-expected NE for maintenance and gain was not influenced (p ≥ 0.15) by silage inclusion treatment. Beef production per hectare was not impacted (p ≥ 0.13) by corn silage inclusion level. Fecal output was increased, and digestibility coefficients for dry matter, organic matter, and crude protein were decreased in CS30 (p ≤ 0.03). Dressing percent and hot carcass weight (HCW) were greater (p ≤ 0.02) in CS15. Implant type did not influence any traits measured (p ≥ 0.14) except for marbling. Marbling was decreased for PLUS (433 vs. 466 ± 17.5; p = 0.02) compared to ONE-F steers. Similar beef produced per hectare of crop land-based upon silage feeding level means producers can feed greater inclusions of corn silage to finishing cattle without impacting carcass quality or beef production; implanting with a coated implant had no detrimental effects to growth performance but increases marbling scores.
Beneficial Effects of L-Carnitine Supplementation during IVM of Canine Oocytes on Their Nuclear Maturation and Development In Vitro
Authors: Moawad Adel R. , Salama Ali , Badr Magdy R. , Fathi Mohamed
Published: February 2021

This study aimed to investigate the effect of L-Carnitine (LC) supplementation during in vitro maturation (IVM) of canine oocytes on nuclear maturation, fertilization status, and preimplantation development. Cumulus–oocyte complexes (COCs) collected from the ovaries of ovariohysterectomized female dogs were matured in vitro for 72 h in a TCM-199 medium supplemented with (0.1, 0.3, 0.6, 1.0, or 2.0 mg/mL) or without (0.0 mg/mL) LC. Matured oocytes were fertilized in vitro with frozen–thawed spermatozoa, and zygotes were cultured in a SOF medium for 7 days. IVM rates were higher (p ≤ 0.05) in 0.3 and 0.6 mg/mL LC supplemented groups than in the control (0.0 mg/mL LC) and other LC groups. Fertilization (18 h postinsemination (pi)) and cleavage (2–16-cell stage at day 3 pi) rates were higher (p ≤ 0.05) in the 0.6 mg/mL LC group than in the control and 0.1, 1.0, and 2 mg/mL LC supplemented groups. Interestingly, 4.5% of fertilized oocytes developed to morula (day 5 pi) in the 0.6 mg/mL LC group, which was higher (p ≤ 0.05) than those developed in the 0.3 mg/mL group (1.0%). No cleaved embryos developed to morula in other groups. In conclusion, LC supplementation at 0.6 mg/mL during IVM of canine oocytes improved their maturation, fertilization, and preimplantation embryo development rates following IVF and in vitro culture (IVC).
The Oxidative Paradox in Low Oxygen Stress in Plants
Authors: Pucciariello Chiara , Perata Pierdomenico
Published: February 2021

Reactive oxygen species (ROS) are part of aerobic environments, and variations in the availability of oxygen (O2) in the environment can lead to altered ROS levels. In plants, the O2 sensing machinery guides the molecular response to low O2, regulating a subset of genes involved in metabolic adaptations to hypoxia, including proteins involved in ROS homeostasis and acclimation. In addition, nitric oxide (NO) participates in signaling events that modulate the low O2 stress response. In this review, we summarize recent findings that highlight the roles of ROS and NO under environmentally or developmentally defined low O2 conditions. We conclude that ROS and NO are emerging regulators during low O2 signalling and key molecules in plant adaptation to flooding conditions.
A Study on Liquid Leak Rates in Packing Seals
Authors: Bouzid Abdel-Hakim
Published: February 2021

The accurate prediction of liquid leak rates in packing seals is an important step in the design of stuffing boxes, in order to comply with environmental protection laws and health and safety regulations regarding the release of toxic substances or fugitive emissions, such as those implemented by the Environmental Protection Agency (EPA) and the Technische Anleitung zur Reinhaltung der Luft (TA Luft). Most recent studies conducted on seals have concentrated on the prediction of gas flow, with little to no effort put toward predicting liquid flow. As a result, there is a need to simulate liquid flow through sealing materials in order to predict leakage into the outer boundary. Modelling of liquid flow through porous packing materials was addressed in this work. Characterization of their porous structure was determined to be a key parameter in the prediction of liquid flow through packing materials; the relationship between gland stress and leak rate was also acknowledged. The proposed methodology started by conducting experimental leak measurements with helium gas to characterize the number and size of capillaries. Liquid leak tests with water and kerosene were then conducted in order to validate the predictions. This study showed that liquid leak rates in packed stuffing boxes could be predicted with reasonable accuracy for low gland stresses. It was found that internal pressure and compression stress had an effect on leakage, as did the thickness change and the type of fluid. The measured leak rates were in the range of 0.062 to 5.7 mg/s for gases and 0.0013 and 5.5 mg/s for liquids.
Plume Divergence and Discharge Oscillations of an Accessible Low-Power Hall Effect Thruster
Authors: Baird Matthew , Kerber Thomas , McGee-Sinclair Ron , Lemmer Kristina
Published: February 2021

Hall effect thrusters (HETs) are an increasingly utilized proportion of electric propulsion devices due to their high thrust-to-power ratio. To enable an accessible research thruster, our team used inexpensive materials and simplified structures to fabricate the 44-mm-diameter Western Hall Thruster (WHT44). Anode flow, discharge voltage, magnet current, and cathode flow fraction (CFF) were independently swept while keeping all other parameters constant. Simultaneously, a Faraday probe was used to test plume properties at a variety of polar coordinate distances, and an oscilloscope was used to capture discharge oscillation behavior. Plasma plume divergence angle at a fixed probe distance of 4.5 thruster diameters increased with increasing anode flow, varying from 36.7° to 37.4°. Moreover, divergence angle decreased with increasing discharge voltage, magnet current, and CFF, by 0.3°, 0.2°, and 8°, respectively, over the span of the swept parameters. Generally, the thruster exhibited a strong oscillation near 90 kHz, which is higher than a similarly sized HET (20–60 kHz). The WHT44 noise frequency spectra became more broadband and the amplitude increased at a CFF of less than 1.5% and greater than 26%. Only the low flow and low voltage operating conditions showed a quiescent sinusoidal discharge current; otherwise, the discharge current probability distribution was Gaussian. This work demonstrates that the WHT44 thruster, designed for simplicity of fabrication, is a viable tool for research and academic purposes.
The Development of Volcanic Ash Cloud Layers over Hours to Days Due to Atmospheric Turbulence Layering
Authors: Bursik Marcus , Yang Qingyuan , Bear-Crozier Adele , Pavolonis Michael , Tupper Andrew
Published: February 2021

Volcanic ash clouds often become multilayered and thin with distance from the vent. We explore one mechanism for the development of this layered structure. We review data on the characteristics of turbulence layering in the free atmosphere, as well as examples of observations of layered clouds both near-vent and distally. We then explore dispersion models that explicitly use the observed layered structure of atmospheric turbulence. The results suggest that the alternation of turbulent and quiescent atmospheric layers provides one mechanism for the development of multilayered ash clouds by modulating vertical particle motion. The largest particles, generally μ>100 μm, are little affected by turbulence. For particles in which both settling and turbulent diffusion are important to vertical motion, mostly in the range of 10–100 μμm, the greater turbulence intensity and more rapid turbulent diffusion in some layers causes these particles to spend greater time in the more turbulent layers, leading to a layering of concentration. The results may have important implications for ash cloud forecasting and aviation safety.
The Role of the Atmospheric Aerosol in Weather Forecasts for the Iberian Peninsula: Investigating the Direct Effects Using the WRF-Chem Model
Authors: Silveira Carlos , Martins Ana , Gouveia Sónia , Scotto Manuel , Miranda Ana I. , Monteiro Alexandra
Published: February 2021

In the atmosphere, aerosols play an important role in climate change, the Earth’s environment and human health. The purpose of this study is to investigate the direct and semi-direct aerosol effects on weather forecasting, focusing on the Iberian Peninsula (IP). To that end, two Weather Research and Forecasting (WRF)-Chem simulations (with and without aerosol feedback) for an entire year (2015) were performed. The model setup includes two nested domains run in two-way mode, allowing the downscaling for the IP domain at a 5 × 5 km2 high-horizontal resolution. The results were explored through agreement of pairs of time series and their spatial variability in order to analyse the importance of including the online-coupled aerosol radiative effect on the meteorological variables: shortwave (solar) radiation, air temperature and precipitation. Significant variations of agreement were found when capturing both temporal and spatial patterns of the analysed meteorological variables. While the spatial distribution of temperature and precipitation is similar throughout the IP domain, with agreement values ranging from 0.87 up to 1.00, the solar radiation presents a distinct spatial pattern with lower agreement values (0.68–0.75) over ocean and higher agreement (0.75–0.98) over land regions. With regard to the spatial differences between simulations, the aerosol contributed to a considerable decrease in annual mean and maximum radiation (up to 20 and 40 Wm−2, respectively), slightly impacting the temperature variation (up to 0.5 °C). These results suggest that the aerosol feedback effects should be accounted when performing weather forecasts, and not only for purposes of air quality assessment.