bluuebunny/crossref_metadata_2025 huggingface dataset:
Non-Invasive Mobile Raman and pXRF Analysis of Armorial Porcelain with the Coat of Arms of Louis XV and Others Enamelled in Canton: Analytical Criteria for Authentication
Authors: Colomban Philippe , Simsek Franci Gulsu , Gallet Xavier
Published: September 2024

Nine glazed porcelain artifacts bearing the coat of arms of France, from King Louis XV tableware orders, were analysed at the laboratory or in their conservation secure room. Based on the experience acquired in the study of 18th century European and Chinese porcelain using mobile XRF (pXRF) and Raman microspectroscopy, a comparison of the impurities in the paste (Y, Rb, and Sr), the elements associated with cobalt in the blue overglaze (Bi, Mn, Zn, and As) and those present in the tin yellow and Naples yellow pigments (Sn, Sb, and Zn) highlights the use of different raw materials for some of these objects. Differences regarding the Ag content in the gold decorations also provide information. Raman identification of the different types of yellow pigment confirms the categorization. The results obtained on the Louis XV tableware are compared to those of “Chine de commande”, attributed to the same places and periods of production or recognized copies. The clustering of the quantitative comparison pXRF signals of the abovementioned elements and a consideration of the Raman parameters of the yellow pigments appear to be effective tools for object categorization to confirm or refute questions about the authenticity of objects.
Effect of Cutting Blade Sharpness on Physical and Nutritional Quality of Fresh-Cut ’Golden Delicious‘ Apples
Authors: Incardona Alessia , Fatchurrahman Danial , Amodio Maria Luisa , Peruzzi Andrea , Colelli Giancarlo
Published: September 2024

The cutting operation significantly affects the shelf-life of fresh-cut produce due to the mechanical damage impacting molecular, physiological, and sensory responses, depending on tissue type and tool characteristics. The degree of sharpness (DoS), defined as the force required to cut a reference body, is crucial for this process. A methodology was developed to objectively evaluate cutting damage on fresh-cut ‘Golden Delicious’ apples using three knives at four DoS levels (30, 100, 140, and 190 N) to cut 96 apples into 288 slices. The study assessed color, visual acceptance score, electrolytic leakage, and nutritional quality over 14 days at 5 °C. A two-way ANOVA showed no significant correlation between DoS and nutritional quality. However, a* values and browning index significantly increased with DoS, with values rising from 39.4 and 2.7 at 30 N to 41.4 and 3.1, respectively, at 190 N. The best visual acceptance score (4.0) and shelf-life (14 days) were at 30 N, while the worst score (2.9) and shelf-life (5 days) were at 190 N. Positive correlations were found between DoS and both browning index and a* value, with coefficients r of 0.97 and 0.93, respectively, highlighting the importance of using sharp tools for optimal post-cutting quality.
Milliwatt μ-TEG-Powered Vibration Monitoring System for Industrial Predictive Maintenance Applications
Authors: Aragonés Raúl , Malet Roger , Oliver Joan , Prim Alex , Mascarell Denis , Salleras Marc , Fonseca Luis , Rodríguez-Iglesias Alex , Tarancón Albert , Morata Alex , Baiutti Federico , Ferrer Carles
Published: September 2024

This paper presents a novel waste-heat-powered, wireless, and battery-less Industrial Internet of Things (IIoT) device designed for predictive maintenance in Industry 4.0 environments. With a focus on real-time quality data, this device addresses the limitations of current battery-operated IIoT devices, such as energy consumption, transmission range, data rate, and constant quality of service. It is specifically developed for heat-intensive industries (e.g., iron and steel, cement, petrochemical, etc.), where self-heating nodes, low-power processing platforms, and industrial sensors align with the stringent requirements of industrial monitoring. The presented IIoT device uses thermoelectric generators based on the Seebeck effect to harness waste heat from any hot surface, such as pipes or chimneys, ensuring continuous power without the need for batteries. The energy that is recovered can be used to power devices using mid-range wireless protocols like Bluetooth 5.0, minimizing the need for extensive in-house wireless infrastructure and incorporating light-edge computing. Consequently, up to 98% of cloud computation efforts and associated greenhouse gas emissions are reduced as data is processed within the IoT device. From the environmental perspective, the deployment of such self-powered IIoT devices contributes to reducing the carbon footprint in energy-demanding industries, aiding their digitalization transition towards the industry 5.0 paradigm. This paper presents the results of the most challenging energy harvesting technologies based on an all-silicon micro thermoelectric generator with planar architecture. The effectiveness and self-powering ability of the selected model, coupled with an ultra-low-power processing platform and Bluetooth 5 connectivity, are validated in an equivalent industrial environment to monitor vibrations in an electric machine. This approach aligns with the EU’s strategic objective of achieving net zero manufacturing capacity for renewable energy technologies, enhancing its position as a global leader in renewable energy technology (RET).
A Maximum Value for the Kullback–Leibler Divergence between Quantized Distributions
Authors: Bonnici Vincenzo
Published: September 2024

The Kullback–Leibler (KL) divergence is a widely used measure for comparing probability distributions, but it faces limitations such as its unbounded nature and the lack of comparability between distributions with different quantum values (the discrete unit of probability). This study addresses these challenges by introducing the concept of quantized distributions, which are probability distributions formed by distributing a given discrete quantity or quantum. This study establishes an upper bound for the KL divergence between two quantized distributions, enabling the development of a normalized KL divergence that ranges between 0 and 1. The theoretical findings are supported by empirical evaluations, demonstrating the distinct behavior of the normalized KL divergence compared to other commonly used measures. The results highlight the importance of considering the quantum value when applying the KL divergence, offering insights for future advancements in divergence measures.
The Structural Aspects of Mutually Trans-X-Cu(I)-X (X = OL, NL, CL, PL, SL, SeL, Cl or Br) Complexes
Authors: Melník Milan , Mikušová Veronika , Mikuš Peter
Published: September 2024

The structural parameters for sixty mutually trans-(X-Cu(I)-X) (X = OL, NL, CL, PL, SL, SeL, Cl or Br, where OL, NL, CL, PL, SL, and SeL are ligands L with respective donor atoms O, N, C, P, S and Se) complexes were analyzed and classified. Within the studied group, there are two types of complexes; the by far most common one is based on coordination, and another one is organometalics based on only C-donor atoms. Linear and bent geometric possibilities exist for coordination number two. The former is dominant in the structures of mutually trans -X–Cu(I)-X. In general, there are three preparative procedures; the most common is the direct reaction of a copper(I) salt with the ligands in a non-aqueous solution (mostly acetonitrile). Copper(I) complex cations can be isolated from salts with larger anions. Unidentate ligands occupy two coordination sites, which results in the linear arrangement. The X–Cu(I)–X angles are between 172.3° and 180°. Overall, it is observed that the mean Cu–X distance increases the covalent radius of the ligating atom in the sequence 1.849 Å (O, 0.73 Å) < 1.886 Å (N, 0.75 Å) < 1.900 Å (C, 0.77 Å) < 2.104 Å (Cl, 0.99 Å) < 2.137 Å (S, 1.02 Å) < 2.236 Å (P, 1.06 Å) < 2.244 Å (Br, 1.14 Å) < 2.260 Å (Se, 1.17 Å).
Realistic Aspects of Cardiac Ultrasound in Rats: Practical Tips for Improved Examination
Authors: Silva Jessica , Azevedo Tiago , Ginja Mário , Oliveira Paula A. , Duarte José Alberto , Faustino-Rocha Ana I.
Published: September 2024

Echocardiography is a reliable and non-invasive method for assessing cardiac structure and function in both clinical and experimental settings, offering valuable insights into disease progression and treatment efficacy. The successful application of echocardiography in murine models of disease has enabled the evaluation of disease severity, drug testing, and continuous monitoring of cardiac function in these animals. However, there is insufficient standardization of echocardiographic measurements for smaller animals. This article aims to address this gap by providing a guide and practical tips for the appropriate acquisition and analysis of echocardiographic parameters in adult rats, which may also be applicable in other small rodents used for scientific purposes, like mice. With advancements in technology, such as ultrahigh-frequency ultrasonic transducers, echocardiography has become a highly sophisticated imaging modality, offering high temporal and spatial resolution imaging, thereby allowing for real-time monitoring of cardiac function throughout the lifespan of small animals. Moreover, it allows the assessment of cardiac complications associated with aging, cancer, diabetes, and obesity, as well as the monitoring of cardiotoxicity induced by therapeutic interventions in preclinical models, providing important information for translational research. Finally, this paper discusses the future directions of cardiac preclinical ultrasound, highlighting the need for continued standardization to advance research and improve clinical outcomes to facilitate early disease detection and the translation of findings into clinical practice.
Analysis of Accidents of Fishing Vessels Caused by Human Elements in Korean Sea Area
Authors: Kim Su-Hyung , Lee Seung-Hyun , Ryu Kyung-Jin , Lee Yoo-Won
Published: September 2024

With an estimated 32,000 annual fatalities, fishing vessel accidents are 100-fold deadlier than those involving merchant ships. Despite ongoing safety training, accident rates remain high. Since most fishing vessel accidents occur in small fishing vessels (<12 m) and are primarily attributable to human elements, this study focuses on small fishing vessel accidents where the human element is the primary cause, exploring preventive measures for major accident types and performing a type-specific risk assessment. First, we performed a quantitative analysis of frequently occurring accidents and the indirect factors influencing the human element using maritime accident statistics and surveys, respectively. Next, we employed the fault tree analysis technique proposed by the International Maritime Organization in its Formal Safety Assessment to quantitatively assess the rate of accidents caused by the human element attributable to various indirect factors. The primary indirect factors most significantly impacting the human element were ship factors (22.8%), people factors (18.9%), and organization on board (17.4%). Secondary factors included personal negligence (14.1%), aging equipment and poor maintenance (10.3%), and harsh natural conditions such as rough waves (9.6%). Eliminating the top three secondary indirect factors reduced accidents due to the human element by 15.4% (64.5−49.1%).
The Generation and Propagation of Wind- and Tide-Induced Near-Inertial Waves in the Ocean
Authors: Li Yang , Xu Zhao , Lv Xianqing
Published: September 2024

Near-inertial waves (NIWs), a special form of internal waves with a frequency close to the local Coriolis frequency, are ubiquitous in the ocean. NIWs play a crucial role in ocean mixing, influencing energy transport, climate change, and biogeochemistry. This manuscript briefly reviews the generation and propagation of NIWS in the oceans. NIWs are primarily generated at the surface by wind forcing or through the water column by nonlinear wave-wave interaction. Especially at critical latitudes where the tidal frequency is equal to twice the local inertial frequency, NIWs can be generated by a specific subclass of triadic resonance, parametric subharmonic instability (PSI). There are also other mechanisms, including lee wave and spontaneous generation. NIWs can propagate horizontally for hundreds of kilometers from their generating region and radiate energy far away from their origin. NIWs also penetrate deep into the ocean, affecting nutrient and oxygen redistribution through altering mixing. NIW propagation is influenced by factors such as mesoscale eddies, background flow, and topography. This review also discussed some recent observational evidence of interactions between NIWs from different origins, suggesting a complicated nonlinear interaction and energy cascading. Despite the long research history, there are still many areas of NIWs that are not well defined.
Optimizing Multi-Quay Combined Berth and Quay Crane Allocation Using Computational Intelligence
Authors: Aslam Sheraz , Michaelides Michalis P. , Herodotou Herodotos
Published: September 2024

The significant increase in international seaborne trade volumes over the last several years is pushing port operators to improve the efficiency of terminal processes and reduce vessel turnaround time. Toward this direction, this study investigates and solves the combined berth allocation problem (BAP) and quay crane allocation problem (QCAP) in a multi-quay (MQ) setting using computational intelligence (CI) approaches. First, the study develops a mathematical model representing a real port environment and then adapts the cuckoo search algorithm (CSA) for the first time in this setup. The CSA is inspired by nature by following the basic rules of breeding parasitism of some cuckoo species that lay eggs in other birds’ nests. For comparison purposes, we implement two baseline approaches, first come first serve and exact MILP, and two CI approaches, particle swarm optimization (PSO) and genetic algorithm (GA), that are typically used to solve such complex or NP-hard problems. Performance assessment is carried out via a comprehensive series of experiments using real-world data. Experimental findings show that the MILP method can address the problems only when a small dataset is employed. In contrast, the newly adapted CSA can solve larger instances of MQ BAP and QCAP within significantly reduced computation times.
A Study on the Bearing Capacity of Square Skirted Anchors with Different Mooring Points
Authors: Wang Yandi , Li Sa , Sun Liqiang , Ren Yuxiao
Published: September 2024

Skirted anchors are often used as temporary or permanent anchoring foundations for underwater pipelines and floating platforms. A series of model tests and finite element simulations were conducted to study the bearing capacity of square skirted anchors with different mooring points. Based on the test results, two failure modes of square skirted anchors with different mooring points were analyzed. It was found that, when the mooring point was located at the top of the side skirt, the square skirted anchor was more prone to rotation and had a lower bearing capacity. The numerical method was validated by the model tests. In total, 140 numerical calculation results show that, when the mooring point position (normalized depth of mooring point) h/H = 0.5~0.75 and the load inclination angle θ = 0°~30°, the bearing performance of the square skirted anchor is optimal. As the aspect ratio of the square skirted anchor (H/B) increases from 0.25 to 2.0, the optimal mooring point position h/H moves downward from 0.5 to 0.75. The failure envelopes in the V-H loading space of the square skirted anchor were drawn, and the corresponding fitting equation was obtained.
Optimization of Controllable-Pitch Propeller Operations for Yangtze River Sailing Ships
Authors: Tian Wuliu , Lang Xiao , Zhang Chi , Yan Songyin , Li Bing , Zang Shuo
Published: September 2024

The Yangtze River’s substantial variation in water depth and current speeds means that inland ships face diverse operational conditions within a single voyage. This paper discusses the adoption of controllable-pitch propellers, which adjust their pitch to adapt to varying navigational environments, thereby optimizing energy efficiency. We developed an optimization framework to determine the ideal pitch angle and rotation speed (RPM) under different sailing conditions. The energy performance model for inland ships was enhanced to account for the open-water efficiency of CPPs across various pitch angles and RPMs, considering the impacts of current and shallow water, among other factors. The optimization approach was refined by incorporating an improved genetic algorithm with an annealing algorithm to enhance the initial population, applying the K-means clustering algorithm for population segmentation, and using multi-parent crossover from diverse clusters. The efficacy of the optimization method for CPP operations was validated by analyzing three operational scenarios of a Yangtze sailing ship. Additionally, key components of the ship performance model were calibrated through experimental tests, demonstrating an anticipated fuel consumption reduction of approximately 5% compared to conventional fixed-pitch propellers.
Calculation of Hydrogen Bonding Enthalpy Using the Two-Parameter Abraham Equation
Authors: Solomonov Boris N. , Khisamiev Mansur B. , Yagofarov Mikhail I.
Published: September 2024

In this work, an approach to the calculation of hydrogen bonding enthalpies is proposed. It employs the correlation proposed by M.H. Abraham, establishing the connection between the equilibrium constant (KHB) and acidity (α2H) and basicity (β2H) parameters: log KHB = 7.354 · α2H · β2H − 1.099. Hydrogen bonding enthalpy (ΔHBH) is found using the compensation relationship with Gibbs energy (ΔHBG): ΔHBG = 0.66 · ΔHBH + 2.5 kJ·mol−1. This relationship enables the calculation of the enthalpy, Gibbs energy and entropy of hydrogen bonding. The validity of this approach was tested against 122 experimental hydrogen bonding enthalpies values available from the literature. The root mean square deviation and average deviation equaled 1.6 kJ·mol−1 and 0.5 kJ·mol−1, respectively.
On the Integration of Complex Systems Engineering and Industry 4.0 Technologies for the Conceptual Design of Robotic Systems
Authors: Restrepo-Carmona Jaime Alonso , Taborda Elkin A. , Paniagua-García Esteban , Escobar Carlos A. , Sierra-Pérez Julián , Vásquez Rafael E.
Published: September 2024

This paper presents a novel integration of Systems Engineering (SE) methodologies and Industry 4.0 (I4.0) technologies in the design of robotic systems, focusing on enhancing underwater robotic missions. Using the conceptual design of an underwater exploration vehicle as a case study, we demonstrate how SE can systematically incorporate I4.0 tools to improve mission performance and meet stakeholder expectations. The study begins with an overview of the SE approach, emphasizing the conceptual design stage and aligning it with the application and case study of design theories. We then explore various I4.0 technologies, highlighting their functional benefits rather than technical specifics and addressing design methods for I4.0. Remotely Operated Vehicles (ROVs) are examined in terms of classification, components, and tasks, showcasing their evolution driven by technological advancements, thus tackling the complexity and design of complex systems. The core of our study involves defining stakeholder expectations, using quality function deployment for requirements definition, and performing a functional and logical decomposition of the ROV system. To deal with design fixation within the design team, we developed a tool to help integrate new technologies by also empathizing with their functional capabilities rather than the technology itself. Our approach underscores the importance of understanding and incorporating new technologies functionally, aligning with the transition towards Industry/Society 5.0. This work not only illustrates the synergy between SE and I4.0, but also offers a structured methodology for advancing the design and functionality of complex systems, setting a blueprint for future developments in this field.
Dynamic Analysis of Multibody Mechanical Systems
Authors: Pappalardo Carmine Maria
Published: September 2024

Dynamic analysis of multibody systems (MBSs) is critical in modern mechanical engineering [...]
Skyrmion Crystal Induced by Four-Spin Interactions in Itinerant Triangular Magnets
Authors: Hayami Satoru
Published: September 2024

We investigate the emergence of magnetic skyrmion crystals with swirling topological spin textures in itinerant magnets with an emphasis on momentum-resolved multi-spin interactions. By performing the simulated annealing for the effective spin model with the two-spin and four-spin interactions on a two-dimensional triangular lattice, we show that various types of four-spin interactions become the microscopic origin of the magnetic skyrmion crystal with the skyrmion numbers of one and two. We find that the four-spin interactions between the different wave vectors lead to the skyrmion crystal with the skyrmion number of one, whereas those at the same wave vectors lead to the skyrmion crystals with the skyrmion number of one and two. Our results indicate that the multi-spin interactions arising from the itinerant nature of electrons provide rich topological spin textures in magnetic metals.
Modeling a Single-Channel Synergetic Observer for Chaos-Based Secure Communication System Applications
Authors: Mushenko Alexey , Pakulova Ekaterina , Basan Elena , Nekrasov Alexey , Gamcová Mária , Kurdel Pavol
Published: September 2024

This paper explores secure communication systems with a chaotic carrier. The use of chaotic oscillations instead of regular van der Pol oscillators as a signal carrier is a promising and active research area, providing not only communication systems with new protection principles and organization but also high steganographic efficiency when transmitting short messages. The problem is to select methods and techniques for mixing a useful signal into a chaotic one and its recovery on the receiver side, featuring a set of properties acceptable for implementation and real-world application. We demonstrate application of synergetic control theory (SCT), which provides advanced observer-basing methods for nonlinear dynamic systems as well as explore example of data transmission system consisting of a Genesio–Tesi chaotic oscillator, data signal transmission with a method of nonlinear modulation, and recovering with a single-channel synergetic observer at the receiver side. The paper presents a nonlinear state observer modeling procedure followed by building a MATLAB/Simulink simulation model of the data transmission system for the PC-platform along with software implementation for the Raspberry Pi platform, with simulation and experimental run results showing data transmission rates seem to be acceptable for the considered practical applications. Practical applications and limitations issues are discussed. Future research will be universal modeling procedures for different classes of chaotic generators and whole system experimental hardware implementation. The obtained results can be primarily used in short messages and/or encryption keys secure transmission systems, cyber-physical system component command communications, as well as chaotic carrier system R&D competitive studies and other applications.
Influence of Framework Material and Abutment Configuration on Fatigue Performance in Dental Implant Systems: A Finite Element Analysis
Authors: Erdoğdu Meryem , Demirel Mehmet Gökberkkaan , Mohammadi Reza , Güntekin Neslihan , Ghanbarzadeh Chaleshtori Masoud
Published: September 2024

Background and Objectives: This study uses finite element analysis to evaluate the impact of abutment angulation, types, and framework materials on the stress distribution and fatigue performance of dental implant systems. Materials and Methods: Three-dimensional models of maxillary three-unit fixed implant-supported prostheses were analyzed. Abutments with different angles and types were used. Two different framework materials were used. Conducted on implants, a force of 150 N was applied obliquely, directed from the palatal to the buccal aspect, at a specific angle of 30 degrees. The distribution of stress and fatigue performance were then assessed, considering the types of restoration frameworks used and the angles of the abutments in three distinct locations. The simulation aspect of the research was carried out utilizing Abaqus Software (ABAQUS 2020, Dassault Systems Simulation Corp., Johnston, RT, USA). Results: In all models, fatigue strengths in the premolar region were higher than in the molar region. Maximum stress levels were seen in models with angled implants. In almost all models with the zirconia framework, fatigue performance was slightly lower. Conclusions: According to the findings of this study, it was concluded that the use of metal-framework multi-unit restorations with minimum angulation has significant positive effects on the biomechanics and long-term success of implant treatments.
Integrating Whey Processing: Ultrafiltration, Nanofiltration, and Water Reuse from Diafiltration
Authors: Brião Vandré Barbosa , Mossmann Juliane , Seguenka Bruna , Graciola Samarah , Piccin Jeferson Steffanello
Published: September 2024

This work proposes an integrated production of whey protein concentrate (WPC) and lactose and the recovery of water from diafiltration (DF) steps. Whey protein and lactose can be concentrated using ultrafiltration and nanofiltration, respectively, and both can be purified using DF. However, DF uses three-fold the initial volume of whey. We propose a method to reclaim this water using reverse osmosis and adsorption by activated carbon. We produced WPC with 88% protein and purified lactose (90%), and 66% of the water can be reclaimed as drinking water. Additionally, the reclaimed water was used to produce another batch of WPC, with no decrease in product quality. Water recovery from the whey process is necessary to meet the needs of a dairy refinery.
A Sub-0.01 °C Resolution All-CMOS Temperature Sensor with 0.43 °C/−0.38 °C Inaccuracy and 1.9 pJ · K2 Resolution FoM for IoT Applications
Authors: Sun Yixiao , Cheng Jie , Luo Zhizhong , Zeng Yanhan
Published: September 2024

A high resolution, acceptable accuracy and low power consumption time-domain temperature sensor is proposed and simulated in this paper based on a 180 nm standard CMOS technology. A diode stacking structure is introduced to enhance the accuracy of the temperature sensing core. To improve the resolution of the sensor, a dual-input capacitor multiplexing voltage-to-time converter (VTC) is implemented. Additionally, a low-temperature drift voltage-mode relaxation oscillator (ROSC) is proposed, effectively reducing the large oscillation frequency drift caused by significant temperature impacts on delay errors. The simulated results show that the resolution is as high as 0.0071 °C over 0∼120 °C with +0.43 °C/−0.38 °C inaccuracy and 1.9 pJ · K2 resolution FoM, consuming only 1.48 μW at a 1.2 V supply voltage.
YOLO-PBESW: A Lightweight Deep Learning Model for the Efficient Identification of Indomethacin Crystal Morphologies in Microfluidic Droplets
Authors: Wei Jiehan , Liang Jianye , Song Jun , Zhou Peipei
Published: September 2024

Crystallization is important to the pharmaceutical, the chemical, and the materials fields, where the morphology of crystals is one of the key factors affecting the quality of crystallization. High-throughput screening based on microfluidic droplets is a potent technique to accelerate the discovery and development of new crystal morphologies with active pharmaceutical ingredients. However, massive crystal morphologies’ datum needs to be identified completely and accurately, which is time-consuming and labor-intensive. Therefore, effective morphologies’ detection and small-target tracking are essential for high-efficiency experiments. In this paper, a new improved algorithm YOLOv8 (YOLO-PBESW) for detecting indomethacin crystals with different morphologies is proposed. We enhanced its capability in detecting small targets through the integration of a high-resolution feature layer P2, and the adoption of a BiFPN structure. Additionally, in this paper, adding the EMA mechanism before the P2 detection head was implemented to improve network attention towards global features. Furthermore, we utilized SimSPPF to replace SPPF to mitigate computational costs and reduce inference time. Lastly, the CIoU loss function was substituted with WIoUv3 to improve detection performance. The experimental findings indicate that the enhanced YOLOv8 model attained advancements, achieving AP metrics of 93.3%, 77.6%, 80.2%, and 99.5% for crystal wire, crystal rod, crystal sheet, and jelly-like phases, respectively. The model also achieved a precision of 85.2%, a recall of 83.8%, and an F1 score of 84.5%, with a mAP of 87.6%. In terms of computational efficiency, the model’s dimensions and operational efficiency are reported as 5.46 MB, and it took 12.89 ms to process each image with a speed of 77.52 FPS. Compared with state-of-the-art lightweight small object detection models such as the FFCA-YOLO series, our proposed YOLO-PBESW model achieved improvements in detecting indomethacin crystal morphologies, particularly for crystal sheets and crystal rods. The model demonstrated AP values that exceeded L-FFCA-YOLO by 7.4% for crystal sheets and 3.9% for crystal rods, while also delivering a superior F1-score. Furthermore, YOLO-PBESW maintained a lower computational complexity, with parameters of only 11.8 GFLOPs and 2.65 M, and achieved a higher FPS. These outcomes collectively demonstrate that our method achieved a balance between precision and computational speed.
Immunological Considerations for the Development of an Effective Herpes Vaccine
Authors: Singer Mahmoud , Husseiny Mohamed I.
Published: September 2024

Research is underway to develop a vaccine to prevent and cure infection from herpes simplex virus (HSV). It emphasizes the critical need for immunization to address public health issues and the shortcomings of existing treatment options. Furthermore, studies on the HSV vaccine advance the field of immunology and vaccine creation, which may help in the battle against other viral illnesses. The current lack of such a vaccine is, in part, due to herpes viral latency in sensory ganglions. Current vaccines rely on tissue-resident memory CD8+ T cells, which are known to provide protection against subsequent HSV reinfection and reactivation without correlating with other immune subsets. For that reason, there is no effective vaccine that can provide protection against latent or recurrent herpes infection. This review focuses on conventional methods for evaluating the efficacy of a herpes vaccine using differential CD8+ T cells and important unaccounted immune aspects for designing an effective vaccine against herpes.
Identification of Bioactive Substances Derived from the Probiotic-Induced Bioconversion of Lagerstroemia speciosa Pers. Leaf Extract That Have Beneficial Effects on Diabetes and Obesity
Authors: An Byung Chull , Kwak Sang Hee , Ahn Jun Young , Won Hye Yeon , Kim Tae Hoon , Ryu Yongku , Chung Myung Jun
Published: September 2024

Lagerstroemia speciosa L. (Banaba) has been used as a functional food because of its diuretic, decongestant, antipyretic, anti-hyperglycemic, and anti-adipogenic activities. Triterpene acids, including corosolic acid, oleanolic acid, and asiatic acid, are the principal phytochemicals in Banaba and are potentially anti-diabetic substances, owing to their effect on blood glucose concentration. Bioconversion of Banaba leaf extract (BLE) by Lactobacillus plantarum CBT-LP3 improved the glucose uptake, insulin secretion, and fat browning of this functional food. Furthermore, we identified asiatic acid, which was found to be increased by 3.8-fold during the L. plantarum CBT-LP3-mediated bioconversion process using metabolite profiling. Most previous studies have focused on corosolic acid, another triterpene acid that is a known anti-diabetic compound and is used to standardize BLE preparations. However, asiatic acid is the second most common of the triterpene acids and is also well known to have anti-diabetic properties. The present study has provided strong evidence that asiatic acid represents an alternative to corosolic acid as the most important active compound. These results suggest that the probiotic-mediated bioconversion of BLE may improve the anti-diabetic effects of this functional food. This implies that the consumption of a probiotic should be encouraged for people undergoing BLE treatment to improve its anti-diabetic effects.
Lingonberry (Vaccinium vitis-idaea L.) Fruit Phenolic Bioactivities—A Review of In Vitro and In Vivo Human Studies
Authors: Pärnänen Pirjo , Niikko Sari , Lähteenmäki Hanna , Räisänen Ismo T. , Tervahartiala Taina , Sorsa Timo , Ranki Annamari
Published: September 2024

This review is focused on the effects of lingonberry (Vaccinium vitis-idaea L.) fruit phenolic compounds in human in vitro cells and in vivo clinical studies. Studies with lingonberries, lingonberry juice/lingonberry nectar/fermented lingonberry juice, and phenolic fractions with active molecules are reviewed. Lingonberry’s bioactive substances have a diverse range of antimicrobial, anti-inflammatory, antiproteolytic, anticancer, and antioxidant properties. Fermentation of lingonberries and modulation of the dysbiotic microbiome to a more symbiotic composition by favoring the growth of lactobacilli and inhibiting the growth of human opportunistic pathogens are discussed. Research results suggest that more studies on humans are needed.
Identification of Virulence Genes Associated with Pathogenicity of Translocating Escherichia coli with Special Reference to the Type 6 Secretion System
Authors: Asgari Behnoush , Burke Jarred R. , Quigley Bonnie L. , Bradford Georgia , Hatje Eva , Kuballa Anna , Katouli Mohammad
Published: September 2024

Recent genomic characterisation of translocating Escherichia coli HMLN-1 isolated from mesenteric lymph nodes (MLNs) and blood of a patient with a fatal case of pancreatitis revealed the presence of a type 6 secretion system (T6SS) that was not present in non-translocating E. coli strains. This strain was also genomically similar to adherent-invasive E. coli (AIEC) LF82 pathotype. We aimed to identify the role of T6SS-1 in the pathogenesis of this strain and other pathogenic E. coli. The HMLN-1 strain was initially tested for the presence of six virulence genes (VGs) associated with AIEC strains and an iron sequestering system. Additionally, HMLN-1’s interaction with a co-culture of Caco-2:HT29-MTX cells and its intra-macrophagic survival was evaluated. We subsequently screened a collection of 319 pathogenic E. coli strains isolated from patients with urinary tract infection (UTI), diarrhoea, inflammatory bowel disease (IBD) and septicaemia for the presence of T6SS-1 and its expression related to adhesion, invasion and translocation via the above co-culture of the intestinal cell lines. The results showed that HMLN-1 harboured four of the AIEC-associated VGs (dsbA, htrA, ompC and afaC). Screening of the pathogenic E. coli collection detected the presence of the T6SS-1 genes in septicaemic and UTI E. coli strains at a significantly higher level than diarrhoea and IBD strains (p < 0.0001). The high expression of T6SS-1 in E. coli HMLN-1 upon adhesion and invasion, as well as its high prevalence among extra-intestinal E. coli strains, suggests a role for T6SS-1 in the pathogenesis of translocating E. coli.
AoRan1 Is Involved in Regulating Conidiation, Stress Resistance, Secondary Metabolism, and Pathogenicity in Arthrobotrys oligospora
Authors: Duan Shipeng , Liu Qianqian , Shen Yanmei , Zhu Lirong , Yuan Hui , Yang Jinkui
Published: September 2024

Arthrobotrys oligospora is a representative nematode-trapping (NT) fungus that is able to capture, kill, and digest nematodes by producing specialized three-dimensional networks (traps) under nutrient-deprived conditions. Ran1 is a serine/threonine protein kinase that can act as a negative regulator of sexual conjugation and meiosis. However, the specific role of Ran1 remains largely unknown in NT fungi. Here, we identified AoRan1 (AOL_s00004g277) via gene disruption, phenotypic analysis, and metabolomic analysis. Our findings reveal that Aoran1 knockout caused a remarkable increase in conidial production, traps, and nematode feeding efficiency. In addition, the absence of Aoran1 resulted in the accumulation of lipid droplets and increased autophagic levels as well as increased tolerance to cell wall synthesis-disturbing reagents and oxidants. Metabolomic analyses also suggested that AoRan1 is involved in multiple metabolic processes, such as fatty acid biosynthesis. In summary, our results suggest that AoRan1 is crucial in conidiation, pathogenicity, and secondary metabolism. This study’s results further our understanding of the molecular mechanisms by which AoRan1 regulates conidiation and trap formation in A. oligospora.
Biotransformation of Sumatriptan by Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Salmonella enterica subsp. enterica
Authors: Jehangir Muhammad , Iqbal Mohammad Saeed , Aftab Usman
Published: September 2024

This study aimed at the biotransformation of sumatriptan by Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Salmonella enterica subsp. enterica and the identification of the drug metabolites by liquid chromatography–mass spectrometry. The drug was incubated with the organisms in tryptic soya broth at 37 °C. The broth was filtered and subjected to liquid chromatography–mass spectrometry. The metabolites identified by the use of mass spectral (+ve ion mode) fragmentation patterns were (3-methylphenyl)methanethiol (Bacillus subtilis), 1-(4-amino-3-ethylphenyl)-N-methylmethanesulfonamide (Salmonella enterica subsp. enterica) and 1-{4-amino-3-[(1E)-3-(dimethylamino)prop-1-en-1-yl]phenyl}methanesulfinamide (Salmonella enterica subsp. enterica, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus). These metabolites exhibit high gastrointestinal absorption, no blood–brain barrier permeability (except (3-methylphenyl)methanethiol), a bioavailability score of 0.55 and no inhibitory effect on CYP2C19, CYP2C9, CYP2D6, CYP3A4 or cytochrome P450 1A2 (except (3-methylphenyl)methanethiol), as determined by SwissADME software ver. 2024. The metabolites appear to be more toxic than the parent drug, as suggested by their calculated median lethal dose values. All four organisms under investigation transformed sumatriptan to different chemical substances that were more toxic than the parent drug.
Effect of Antisolvent Used to Regenerate Cellulose Treated with Ionic Liquid on Its Properties
Authors: Bloch Marta , Woźniak Magdalena , Dwiecki Krzysztof , Borysiak Sławomir , Ratajczak Izabela
Published: September 2024

The solvolysis reaction with ionic liquids is one of the most frequently used methods for producing nanometer-sized cellulose. In this study, the nanocellulose was obtained by reacting microcrystalline cellulose with 1-ethyl-3-methylimidazolium acetate (EmimOAc). The aim of this research was to determine the influence of various antisolvents used in the regeneration of cellulose after treatment with ionic liquid on its properties. The following antisolvents were used in this research: acetone, acetonitrile, water, ethanol and a mixture of acetone and water in a 1:1 v/v ratio. The nanocellulose was characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), dynamic light scattering (DLS), scanning electron microscopy (SEM) and elemental analysis (EA). The results show that the antisolvent used to regenerate cellulose after the solvolysis reaction with EmimOAc affects its properties. Water, ethanol and a mixture of acetone and water successfully removed the used ionic liquid from the cellulose structure, while acetone and acetonitrile were unable to completely remove EmimOAc from the cellulosic material. The results of the XRD analysis indicate that there is a correlation between the ionic liquid content in the regenerated cellulose and its degree of crystallinity. Among the tested solvents, water leads to the effective removal of EmimOAc from the cellulose structure, which is additionally characterized by the smallest particle size and non-formation of agglomerates.
Plant Phenolics in the Prevention and Therapy of Acne: A Comprehensive Review
Authors: Koch Wojciech , Zagórska Justyna , Michalak-Tomczyk Magdalena , Karav Sercan , Wawruszak Anna
Published: September 2024

Plants are a rich source of secondary metabolites, among which phenolics are the most abundant. To date, over 8000 various polyphenolic compounds have been identified in plant species, among which phenolic acids, flavonoids, coumarins, stilbenes and lignans are the most important ones. Acne is one of the most commonly treated dermatological diseases, among which acne vulgaris and rosacea are the most frequently diagnosed. In the scientific literature, there is a lack of a detailed scientific presentation and discussion on the importance of plant phenolics in the treatment of the most common specific skin diseases, e.g., acne. Therefore, the aim of this review is to gather, present and discuss the current state of knowledge on the activity of various plant phenolics towards the prevention and treatment of acne, including in vitro, in vivo and human studies. It was revealed that because of their significant antibacterial, anti-inflammatory and antioxidant activities, phenolic compounds may be used in the treatment of various types of acne, individually as well as in combination with commonly used drugs like clindamycin and benzoyl peroxide. Among the various phenolics that have been tested, EGCG, quercetin and nobiletin seem to be the most promising ones; however, more studies, especially clinical trials, are needed to fully evaluate their efficacy in treating acne.
Mapping of Some Further Alkylation-Initiated Pathways to Polyheterocyclic Compounds from Indigo and Indirubin
Authors: Ali Sarfaraz , McCosker Patrick M. , Willis Anthony C. , Pyne Stephen G. , Richardson Christopher , Bremner John B. , Keller Paul A.
Published: September 2024

The reaction of indigo with two equivalents of the electrophile ethyl bromoacetate with caesium carbonate as a base result in the formation of structurally complex polyheterocyclics, including a fused spiroimidazole and a spiro[1,3]oxazino derivative, together with a biindigoid-type derivative, through a convenient one-pot reaction. Further assessment of the reaction using five equivalents of the electrophile gave rise to other molecules incorporating the 2-(7,13,14-trioxo-6,7,13,14-tetrahydropyrazino[1,2-a:4,3-a′]diindol-6-yl) scaffold. The reaction of ethyl bromoacetate with the less reactive indirubin resulted in the synthesis of three derivatives of a new class of polyheterocyclic system via a cascade process, although yields were low. These compounds were derived from the parent indolo[1,2-b]pyrrolo[4,3,2-de]isoquinoline skeleton. Despite the modest yields of the reactions, they represent quick cascade routes to a variety of heterocycles from cheap starting materials, with these structures otherwise being difficult to synthesise in a traditional stepwise manner. These outcomes also contribute significantly to the detailed understanding of the indigo/indirubin cascade reaction pathways initiated by base-catalysed N-alkylation.
The ATTO 565 Dye and Its Applications in Microscopy
Authors: Wu Yuheng , Williams René M.
Published: September 2024

ATTO 565, a Rhodamine-type dye, has garnered significant attention due to its remarkable optical properties, such as a high fluorescence quantum yield, and the fact that it is a relatively stable structure and has low biotoxicity. ATTO 565 has found extensive applications in combination with microscopy technology. In this review, the chemical and optical properties of ATTO 565 are introduced, along with the principles behind them. The functionality of ATTO 565 in confocal microscopy, stimulated emission depletion (STED) microscopy, single-molecule tracking (SMT) techniques, two-photon excitation–stimulated emission depletion microscopy (TPE-STED) and fluorescence correlation spectroscopy (FCS) is discussed. These studies demonstrate that ATTO 565 plays a crucial role in areas such as biological imaging and single-molecule localization, thus warranting further in-depth investigations. Finally, we present some prospects and concepts for the future applications of ATTO 565 in the fields of biocompatibility and metal ion detection. This review does not include theoretical calculations for the ATTO 565 molecule.
The Burden of Sleep/Wake Disorders: Excessive Daytime Sleepiness and Insomnia Project
Authors: Tüzün Marina , Kallweit Ulf , Seidel Stefan , Endrich Olga , Trelle Sven , Leone Maurizio A. , Bruni Oliviero , Dodel Richard , Konti Maria , Lolich Maria , Pupillo Elisabetta , Ramankulov Dauren , Vignatelli Luca , Meyer-Massetti Carla , Schmidt Markus , Bassetti Claudio L. A.
Published: September 2024

Excessive daytime sleepiness (EDS) and insomnia (IN) complaints represent the most common sleep/wake disorders. Currently, the specific needs of these patients and their relatives, as well as the overall socio-economic burden of IN and EDS remains widely unexplored. This pilot study to be carried out in Switzerland is a retro- and prospective, national, one-center cohort observational study for the systematic evaluation of the burden of EDS and IN and its evolution 12 months after the first assessment. Patient recruitment will be organized through 7–8 primary care providers (primary/general care practitioners and pharmacies). Primary outcomes are the prevalence of EDS/IN in the primary care setting and the association between EDS/IN with health-related quality of life (QOL) as assessed with the established instruments. Secondary outcomes are the association between EDS/IN with the presence of comorbidities, number of injuries/accidents, and number of sick/leave days for the subgroup of working subjects. Calculation of direct per-patient costs will be undertaken to analyze the economic implications of sleep/wake disorders, providing valuable insights into the financial burden experienced by affected individuals within the healthcare system. This research will provide information on the feasibility of such a study and inform on aspects of the QOL most associated with EDS/IN. Based on this pilot project, a European multicenter study on the burden of sleep/wake disorders will be conducted by the European Academy of Neurology.