bluuebunny/crossref_metadata_2025 huggingface dataset:
STUDI KEPUASAN PENGGUNA LULUSAN PROGRAM STUDI PENDIDIKAN TEKNIK BANGUNAN UNIVERSITAS NEGERI JAKARTA DI BIDANG KEPENDIDIKAN
Authors: Nda Nida Zafarina
Published: September 2020

Nida Zafarina. Studi Kepuasan Pengguna Lulusan Program Studi Pendidikan Teknik Bangunan Universitas Negeri Jakarta Di Bidang Kependidikan. Skripsi. Jakarta: Program Studi Pendidikan Teknik Bangunan Fakultas Teknik Universitas Negeri Jakarta. Agustus 2019.

Penelitian ini bertujuan untuk mengetahui profil lulusan Program Studi Pendidikan Teknik Bangunan Universitas Negeri Jakarta terhadap bidang kerja kependidikan sebagai guru dan untuk mengetahui kepuasan pengguna lulusan terhadap kinerja lulusan. Penelitian ini dilakukan terhitung dari bulan Maret sampai dengan Juli 2019.

Penelitian dilaksanakan terhadap lulusan Program Studi Pendidikan Teknik BangunanUniversitas Negeri Jakarta, penelitian ini menggunakan metode kuantitatif yang berbentuk angka dan teknik pengambilan data dengan cara menyebar kuesioner kepada lulusan melalui google form agar lebih mudah mendapatkan jawaban dan menyebar langsung kuesioner kepada pengguna lulusan untuk mengetahui kinerja lulusan Pendidikan Teknik Bangunan. Sampel pada penelitian ini sebanyak 23 lulusan yang bekerja dibidang kependidikan sebagai guru.

Hasil yang didapatkan dari studi penelusuran lulusan Pendidikan Teknik Bangunan yaitu (69,6%) lulusan bekerja di SMK , (52%) menyatakan bahwa pekerjaan saat ini sesuai dengan bidang ilmu yang didapatkan selama kuliah. Pada Kepuasan Pengguna Lulusan terhadap penilaian kinerja lulusan sesuai dengan Kompetensi lulusan dibuktikan bahwa kompetensi pedagogik (40%) kategori sedang, kompetensi kepribadian (62%) kategori tinggi, kompetensi profesional (62%) kategori tinggi , dan kompetensi sosial (42%) kategori sedang. Hasil  penelitian tersebut menunjukan bahwa pengguna lulusan puas dengan kinerja lulusan PTB UNJ pada kompetensi kepribadian dan kompetensi profesional. Sementara itu pengguna lulusan belum merasa puas dengan kompetensi pedagogik dan kompetensi sosial.
Metode Grafis untuk Penggambaran Diagram Geser dan Momen pada Balok
Authors: Lim Edwin , Lingga Andry Alim
Published: September 2020

Statika merupakan ilmu dasar yang harus dikuasai oleh mahasiswa(i) Teknik Sipil sebelum mengambil mata kuliah mekanika teknik lanjut. Salah satu topik yang sering kali menjadi hambatan bagi mahasiswa(i) adalah topik penggambaran bidang geser dan momen. Selain sifatnya yang relatif abstrak, metode yang sering diperkenalkan untuk penggambaran bidang geser dan momen cenderung rumit karena mahasiswa(i) perlu memotong balok menjadi freebody – freebody yang dapat digunakan untuk perumusan persamaan bidang geser dan momen (metode persamaan). Jikalau mahasiswa(i) dapat merumuskan persamaan bidang geser dan momen secara benar, masih terdapat kemungkinan bahwa bidang geser dan momen yang digambarkan kelirukarena ketidakpahaman akan sifat persamaan. Kesulitan ini dapat dihilangkan dengan menawarkan metode grafis dalam penggambaran bidang geser dan momen kepada mahasiswa(i) sebagai metode alternatif atau pendukung. Metode grafis menerapkan enam aturan yang diturunkan berdasarkan relasi antara beban luar, bidang geser, dan bidang momen. Metode grafis ini diperkenalkan kepada mahasiswa(i) yang mengambil mata kuliah Statika I: StrukturStatisTertentu di Universitas Tanjungpura, Pontianak. Hasil analisis terhadap nilai ujian akhir pada tiga kelas yang berbeda menunjukkan perbedaan signifikan yang positif untuk penerapan metode grafis kepada mahasiwa(i) di Universitas Tanjungpura.
MODELLING 2-D PONDASI JEMBATAN BERDASARKAN PEMBEBANAN DAN LETAK POSISI BATUAN ‘X’ BERDASARKAN PROGRAM GRAPHIC USER INTERFACE BERBASIS MATLAB
Authors: Risal Ardiansyah Putra  , Fachri Almawali Abil FIda  , Widya Utama
Published: September 2020

This paper contains an analysis of the loading and the position of rock in the bridge foundation based on the rock's response to the force that will be tested with "Graphic User Interface (GUI)" program using MATLAB. This paper also explains the preparation of bridge foundation modeling based on the forces tested on the rock named ‘x’. The response forces discussed in this paper are normal force, latitude force and moment force. The data is processed using MATLAB software and then presented in the form of tables, graphs and 2D modeling that shows the loading and the position of rocks correspondence to the bridge foundation. After obtaining the rock’s ‘x’ data, the data is then processed using Graphic User Interface program, through the program moment force graphs, normal force graphs, latitude graphs can be obtained which later these results can be considered for modeling the loading and the position of the rock’s ‘x’. Through the data processing that has been done, zero normal force is obtained, and the latitude has a value of about (0 to 8) N and the moment force of about (-60 to 0) N. Based on these calculations it was found that the rock’s 'x' can be modeled by arranging it rectangle with center of gravity evenly distributed at the center. And the model that are used is the placement pinch model because it does not have translational and rotational values ​​so that the rock ‘x’ is suitable to be the bridge's foundation.
Pengembangan E-Modul Berbasis Android Mata Kuliah Struktur Beton II
Authors: Rachmi Anggita
Published: September 2020

This study is a research and development which has the aim to develop learning instructional materials in the form of Android-based e-modules in Concrete Structure II. The method used in this research is Research and Development with ADDIE model. This development model has 5 stages, namely analyzing, designing, developing, implementing, and evaluating. However, this research only applies to the development stage. The measuring instrument used is an instrument to measure the feasibility of teaching materials by material experts and media expert. This research was carried out by material experts value was 93,26% which could be categorized as "Very Decent". While the value by media experts obtained a value of 97,22% which can be categorized as "Very Decent".
Pengembangan Multimedia Interaktif Mata Kuliah Ilmu Ukur Tanah I
Authors: Denirian R.R Ayyub , Prihantono
Published: September 2020

This study aims to produce learning media, namely interactive multimedia with web-based, in the Soil Measuring Science course in Building Engineering Education Study Program. Interactive multimedia developed is a tutorial model with a function as a complement in learning. The method used in this research is research and development that refers to the Lee and Owens instructional design model. The Lee and Owen model consists of five stages, including assessment / analysis, design, development, implementation, and evaluation. Data analysis techniques in this study used a questionnaire (questionnaire) to collect data from needs analysis, validation of media and material experts, and product evaluation by users. The results of this development research in the form of five interactive multimedia, interactive multimedia is validated by two media experts and two material experts. Based on the results of the validation by media experts, a value of 81% is categorized as very feasible, and based on the results of the validation by the material experts, a score of 84.41% is obtained. The user assessment results get a value of 4.79 included in the excellent category. Based on these values this media can already be used in the Soil Measuring Science course.
Penentuan Model Terbaik untuk Peramalan Data Saham Closing PT. CIMB Niaga Indonesia Menggunakan Metode Arch-Garch
Authors: Eka Gatri , Safitry Vebriani , Kristin Yesika
Published: September 2020

This study explains about forecasting model stock closing price data PT. CIMB Niaga Indonesia on June 2017. The goal of this research is to formulated forecasting model stock data of PT. CIMB Niaga Indonesia specially is that stock closing price data by using ARCH/GARCH model. The result that got is that ARCH(1) model is the suitable model to forecasting stock closing price data. Result of forecasting for daily later so that stock data specially stock closing price is being increase.
ANALISIS GEOGRAPHICALLY WEIGHTED REGRESSION (GWR) PADA KASUS PNEUMONIA BALITA DI PROVINSI JAWA BARAT
Authors: Nadya Meila , Rahayu Widyanti , Santi Vera Maya
Published: September 2020

Pneumonia adalah salah satu penyakit Infeksi Saluran Pernapasan Akut (ISPA) yang disebabkan oleh bakteri atau virus. Di Indonesia, pneumonia merupakan penyebab kematian balita tertinggi kedua setelah diare. Kasus pneumonia pada balita terbanyak yang ditemukan di Indonesia adalah di provinsi Jawa Barat. Untuk mengatasi pneumonia balita perlu dianalisis faktor penyebab pneumonia balita. Salah satu cara untuk menganalisis faktor tersebut adalah dengan menggunakan analisis Geographically Weighted Regression (GWR). Analisis GWR merupakan pengembangan dari analisis regresi linier berganda yang dapat mengatasi keragaman wilayah/heterogenitas spasial sehingga menghasilkan model dan pendugaan parameter berbeda untuk setiap wilayah amatan. Hasil analisis GWR dengan menggunakan pembobot spasial \textit{Fixed Kernel Gaussian} menunjukkan bahwa model GWR lebih baik daripada model regresi linier berganda. Hal ini berdasarkan nilai R2, nilai R2 dari model GWR (88.34%) lebih besar dari R2 dari model regresi linier berganda (71.86%). Sementara, Jumlah Kuadrat Galat (JKG) untuk model GWR diperoleh 3.031 lebih kecil bila dibandingkan dengan nilai JKG dari regresi linier berganda yang sebesar 7.317. Secara umum, terdapat tiga faktor yang berpengaruh signifikan terhadap kasus pneumonia balita di provinsi Jawa Barat tahun 2014 yaitu jumlah balita gizi buruk, persentase bayi yang diimunisasi dasar lengkap dan jumlah puskesmas.
Aplikasi Model Vector Autoregressive (VAR) pada Data Tamu Mancanegara di Hotel Bintang dan Non Bintang di Daerah Istimewa Yogyakarta
Authors: Sihombing Pardomuan , Bekti Endar Susilowati
Published: September 2020

Model Vector Autoregressive (VAR) merupakan gabungan dari beberapa model Autoregressive (AR), dimana model membentuk sebuah vektor yang antara variabel-variabelnya saling memengaruhi. Model AR(1) menyatakan bahwa pengamatan waktu sekarang dipengaruhi pengamatan satu waktu sebelumnya dan unsur error. Pada analisis ini, model Vector Autoregressive (VAR) digunakan pada data tamu mancanegara per bulan yang menginap di Hotel Bintang dan Non bintang di Daerah Istimewa Yogyakarta per bulan periode Januari 2008 sampai dengan Desember 2015. Pembentukan model VAR melalui beberapa tahap yaitu: uji stasioneritas, penentuan orde autoregressive, pembentukan model VAR, dan diagnostic checking. Untuk pengolahan data dilakukan dengan program R 3.5.1. Dari analisis data, variabel jumlah tamu wisatawan mancanegara di Hotel Bintang dan Hotel Non Bintang di Daerah Istimewa Yogyakarta memiliki korelasi yang cukup tinggi yaitu sebesar 0,91. Dengan model Vector Autoregressive (VAR) yaitu VAR(1) didapatkan kedua hasil persamaan simultan yang signifikan. Nilai R2 dan Adjusted R2 kedua persamaan parsial model VAR(1) cukup tinggi yaitu untuk persamaan variabel Hotel Bintang didapatkan R2 sebesar 71,13% dan Adjusted R2 70,5%, sedangkan untuk persamaan variabel Hotel Non Bintang didapatkan R2 sebesar 76,56% dan dan Adjusted R2 70,65 %.
PEMAHAMAN PELATIH FUTSAL LIGA AAFI U16 PADA PERATURAN PERTANDINGAN SESUAI MANUAL LIGA
Authors: Ahmad Hasbullah Amza  , Fitranto Nur
Published: September 2020

ABSTRAK

 

Penelitian ini bertujuan untuk menentukan tingkat pemahaman pelatih Futsal regional Jakarta tentang peraturan pertandingan Futsal di Manual League AAFI U16. Penelitian ini dilakukan melalui pengumpulan data dengan menyebarkan kuesioner selama pertemuan pelatih Futsal akademik yang diadakan pada 13 - 14 Juli 2019. Penelitian ini dikategorikan sebagai penelitian kuantitatif deskriptif, dengan metode survei menggunakan kuesioner sebagai alat. Subjek penelitian ini adalah pelatih Futsal 70 regional AAFA U16 liga Jakarta. Teknik purposive sampling digunakan dalam penelitian ini sebagai metode pengumpulan sampel untuk sebanyak 30 orang yang diwawancarai. Pengumpulan data menggunakan kuesioner tertutup. Hasil analisis data menunjukkan bahwa pengetahuan tentang aturan pertandingan Futsal oleh pelatih liga AAFI U16 regional Jakarta adalah; 26 orang (65%) memiliki pengetahuan besar dan 14 orang (35%) memiliki cukup atau cukup menengah.

 

Kata kunci: Pengetahuan tentang Peraturan Pertandingan, Pelatih, Futsal

 

ABSTRACT

 

               This research aims to determine the level of understanding of Jakarta’s regional Futsal coaches regarding Futsal match regulations in Manual League AAFI U16. This research was done through data collecting by distributing questionaires during the meetings of academic Futsal coaches that were held on 13 – 14 July 2019. This research is categorized as a descriptive quantitative research, with survey method using questionaires as tools. Rthe subject of the research are the Jakarta’s regional Futsal 70 coaches of AAFI U16 league. Purposive sampling technique was used in this research as sample collecting method to as many as 30 interviewees. Data collection using closed questionaires. The data analysis result shown that the knowledge regarding rules of Futsal match by the Jakarta’s regional AAFI U16 league coaches are; 26 people (65%) possesses great knowledge and 14 people (35%) possesses sufficient or enough intermediate.

 

Keywords: Knowledge of Match Rules, Coaches, Futsal
Usefulness of an Accelerometer-Based Portable Navigation System for Total Knee Arthroplasty
Authors: Minoda Yukihide , Hayakawa Kazue , Hagio Keisuke , Konishi Natsuo , Tamaki Tatsuya , Iwakiri Kentaro ,   the KneeAlign 2 Multicenter RCT Group
Published: September 2020

Background:

An accelerometer-based portable navigation system was recently introduced to improve prosthetic alignment during total knee arthroplasty (TKA). The purpose of this multicenter prospective randomized controlled trial (RCT) was to evaluate the effects of this accelerometer-based portable navigation system for achieving more accurate alignment during TKA in the clinical setting.

Methods:

One hundred patients with primary varus osteoarthritis of the knee were enrolled in this prospective RCT conducted in 5 hospitals. A navigation system was utilized in 50 patients (navigation group), and a conventional intramedullary femoral guide and an extramedullary tibial guide were utilized in 50 patients (conventional group). At 6 months postoperatively, weight-bearing radiographs were obtained of the whole operative leg. An experienced surgeon who was blinded to the treatment assignments then measured the alignment to 1 decimal place with use of computer software. Power analysis showed that 41 knees were required in each group.

Results:

There were no complications as a result of the use of the accelerometer-based portable navigation system. Postoperative radiographs were obtained in 45 patients from each group. There were no significant differences in sex, age, height, body weight, body mass index, preoperative femorotibial angle, and operative time between groups. The absolute differences of the femoral prosthesis (p = 0.01), tibial prosthesis (p < 0.01), and hip-knee-ankle angle (p < 0.01) from a neutral mechanical axis were less in the navigation group compared with those in the conventional group. Alignment outliers (>2° away from the neutral mechanical axis) of the tibial prosthesis and hip-knee-ankle angle were less in the navigation group (9% and 27%, respectively) compared with those in the conventional group (31% and 49%; p = 0.01 and p = 0.04, respectively).

Conclusions:

To our knowledge, this is the first multicenter prospective RCT to evaluate an accelerometer-based portable navigation system. An accelerometer-based portable navigation system provides more accurate prosthetic and limb alignment in the coronal plane than conventional techniques, without extended operative time or an increased rate of complications. The results of this study may help orthopaedic surgeons decide whether or not to use an accelerometer-based portable navigation system.

Level of Evidence:

Therapeutic Level I. See Instructions for Authors for a complete description of levels of evidence.
Harmonisasi Hak Kepegawaian dalam Peraturan Perundang-undangan bagi Penyandang Disabilitas Terkait Kuota di Daerah Istimewa Yogyakarta
Authors: Harahap Nurmalita Ayuningtyas , M. Syafi'ie
Published: September 2020

AbstractLegal protection related to the rights of persons with disabilities still causes problems due to various regulations concerning persons with disabilities in the Special Region of Yogyakarta (DIY) are still not harmonious. This research tries to xpolre the question, is the regulation regarding employment rights for persons with disabilities relate to fulfilling qootas to become civil servants in DIY harmonious? And what kind of legal remedies should be done as a form of harmonization of laws and regulations regarding the rights of persons with disabilities related to employment rights in DIY? This research is a normative research, that is the research object of study in the form of legal norms. Primary and secondary materials is obtained through literature study, and analysis of the data will be analyzed legally. The result of the study concluded, the regulation of employment rights for person with disabilities relating to minimum amout of recruitment quota in the government agencies in DIY is not harmonius, and legal remedies can be made with legislative review.Key Words: Persons with disabilities, employment rights, harmonization

AbstrakPerlindungan hukum terkait dengan hak penyandang disabilitas masih menimbulkan permasalahan, disebabkan oleh berbagai peraturan tentang penyandang disabilitas di Daerah Istimewa Yogyakarta (DIY) masih belum harmonis. Penelitian ini mencoba menggali pertanyaan yaitu, apakah pengaturan mengenai hak kepegawaian bagi penyandang disabilitas terkait pemenuhan kuota untuk menjadi pegawai negeri di DIY sudah harmonis? dan seperti apa upaya hukum yang harus dilakukan sebagai bentuk harmonisasi peraturan perundang-undangan mengenai hak-hak penyandang disabilitas terkait dengan hak kepegawaian di DIY? Penelitian ini merupakan penelitian normatif, yakni penelitian yang obyek kajiannya berupa norma hukum. Bahan primer dan sekunder diperoleh melalui studi kepustakaan. Analisa data akan dianalisis secara yuridis. Hasil penelitian menyimpulkan bahwa, pengaturan hak pekerjaan bagi penyandang disabilitas yang berkenaan dengan besarnya minimal kuota penerimaan pegawai di instansi pemerintahan di DIY belum harmonis dan upaya hukum yang dapat dilakukan dengan legislative review.
Pemahaman Masyarakat Pesisir Lampung akan Bahaya Harmful Algae Bloom pada Sumber Pangan Laut
Authors: Hidayati Inayah
Published: September 2020

Harmful Algae Bloom (HABs) menyebabkan berbagai dampak pada kehidupan laut terutama kejadian kematian fauna serta secara tidak langsung berpengaruh pada kesehatan manusia yang mengkonsumsi sumber pangan laut. Penelitian ini penting untuk dilakukan karena masyarakat pesisir menggantungkan kehidupannya pada hasil laut serta mengkonsumsi sumber pangan laut.  Secara umum, masyarakat pesisir Lampung telah mengetahui adanya HABs namun belum semua memahami mengapa dan bagaimana peristiwa tersebut terjadi secara mendalam serta dampak bagi kesehatan manusia. Tujuan penelitian adalah untuk mengetahui tingkat pemahaman masyarakat akan bahaya HABs yang berdampak pada sumber pangannya. Data yang digunakan dalam penelitian ini adalah data survei yang didukung dengan hasil wawancara mendalam dan focus group discussion. Hasil kajian menunjukkan bahwa lebih dari separuh responden pernah mengetahui informasi dan bahaya mengenai HABs (57 persen). Penyebaran informasi mengenai kejadian dan penanganan HABs di Lampung belum begitu bagus. Saat terjadi HABs, hanya 54 persen masyarakat yang mengetahui terjadinya kematian ikan karena peristiwa tersebut. padahal kematian ikan tersebut terjadi kurang lebih selama 3 hari berturut-turut. Media yang diharapkan menyebarluaskan informasi kejadian HABs belum begitu berperan, masyarakat yang mengetahui berita kematian ikan sebagian besar karena melihat langsung. Sedikit sekali masyarakat yang mengetahui berita kematian ikan akibat HABs melalui media cetak maupun elektronik.

Harmful Algae Bloom (HABs) causes various impacts on sea life, especially the incidence of fauna death and indirectly affects the health of humans who consume seafood sources. This research is essential to do because coastal communities depend their lives on marine products and consume seafood sources. In general, Lampung coastal communities are aware of the existence of HABs, but not all understand why and how these events occur in-depth and the impact on human health. The research objective is to determine the level of public understanding of the dangers of HABs that have an impact on food sources. The data used in this study are survey data supported by focus group discussions. The results of the study showed that more than half of respondents had known information and hazards regarding HABs (57 per cent). Dissemination of information about the incidence and handling of HABs in Lampung is not very good. When there were HABs, only 54 per cent of the people were aware of fish deaths due to these events.  The media which is expected to disseminate information on the HABs incident has not yet played a significant role, most of the people who know about the news of fish deaths have seen it firsthand. Very few people know about the news of fish deaths due to HABs through print and electronic media.
Computer simulation of overvoltages in 6–10 kV distribution networks for single-phase ground faults
Authors: Kurochka K. S. , Tokochakov V. I. , Karabchikova E. A. , Panaryn K. A.
Published: September 2020

Nowadays, when designing and operating electric power systems, special attention is paid to predicting emergency situations and minimizing losses in case they occur. The most common method of overvoltage investigations is the use of simulation models in specialized application packages, which imposes additional qualification requirements on the electrical engineer.

The authors propose modeling technologies and a software tool for modeling and research of overvoltages that occur in 6–10 kV distribution networks at single-phase ground faults, including the method of visual design of the distribution network through the use of information technology and automated generation of mathematical model for its further study to analyze overvoltages.
Upregulation of miR-205 induces CHN1 expression, which is associated with the aggressive behaviour of cervical cancer cells and correlated with lymph node metastasis
Authors: liu jianbing , li yunfeng , chen xihua , xu xiangbo , zhao haoqi , wang shufang , hao jianqing , he bin , liu shuyan , wang jiedong
Published: September 2020

Background: Cervical cancer is the leading cause of cancer-related death in women worldwide. However, the mechanisms mediating the development and progression of cervical cancer are unclear. In this study, we aimed to elucidate the roles of microRNAs and a1-chimaerin (CHN1) protein in cervical cancer progression. Methods: The expression of miR-205 and CHN1 protein was investigated by in situ hybridisation and immunohistochemistry. We predicted the target genes of miR-205 using software prediction and dual luciferase assays. The expression of mRNAs and proteins was tested by qRT-PCR and western blotting respectively. The ability of cell growth, migration and invasion was evaluated by CCK-8 and transwell. Cell apoptosis was analysed by flow cytometry analysis. Results: We found that miR-205 and CHN1 were highly expressed in human cervical cancer tissue compared with paired normal cervical tissues. The CHN1 gene was shown to be targeted by miR-205 in HeLa cells. Interestingly, transfection with miR-205 mimic upregulated CHN1 mRNA and protein, while miR-205 inhibitor downregulated CHN1 in high-risk and human papilloma virus (HPV)-negative human cervical cancer cells in vitro,. These data suggested that miR-205 positively regulated the expression of CHN1. Furthermore, the miR-205 mimic promoted cell growth, apoptosis, migration, and invasion in high-risk and HPV-negative cervical cancer cells, while the miR-205 inhibitor blocked these biological processes. Knockdown of CHN1 obviously reduced the aggressive cellular behaviours induced by upregulation of miR-205, suggesting that miR-205 positively regulated CHN1 to mediate these cell behaviours during the development of cervical cancer. Furthermore, CHN1 was correlated with lymph node metastasis in clinical specimens. Conclusions: Our findings showed that miR-205 positively regulated CHN1 to mediate cell growth, apoptosis, migration, and invasion during cervical cancer development, particularly for high-risk HPV-type cervical cancer. These findings suggested that dysregulation of miR-205 and subsequent abnormalities in CHN1 expression promoted the oncogenic potential of human cervical cancer.
Latent-space embedding of expression data identifies gene signatures from sputum samples of asthmatic patients
Authors: LOU Shaoke , Li Tianxiao , Spakowicz Daniel , Yan Xiting , Chupp Geoffrey Lowell , Gerstein Mark
Published: September 2020

Background: The pathogenesis of asthma is a complex process involving multiple genes and pathways. Identifying biomarkers from asthma datasets, especially those that include heterogeneous subpopulations, is challenging. Potentially, autoencoders provide ideal frameworks for such tasks as they can embed complex, noisy high-dimensional gene expression data into a low-dimensional latent space in an unsupervised fashion, enabling us to extract distinguishing features from expression data.Results: Here, we developed a framework combining a denoising autoencoder and a supervised learning classifier to identify gene signatures related to asthma severity. Using the trained autoencoder with 50 hidden units, we found that hierarchical clustering on the low-dimensional embedding corresponds well with previously defined and clinically relevant clusters of patients. Moreover, each hidden unit has contributions from each of the genes, and pathway analysis of these contributions shows that the hidden units are significantly enriched in known asthma-related pathways. We then used genes that contribute most to the hidden units to develop a secondary random-forest classifier for directly predicting asthma severity. The feature importance metric from this classifier identified a signature based on 50 key genes, which are associated with severity. Furthermore, we can use these key genes to successfully estimate FEV1/FVC ratios across patients, via support-vector-machine regression. Conclusion: We found that the denoising autoencoder framework can extract meaningful patterns corresponding to functional gene groups and patient clusters from the gene expression of asthma patients.
Association between physical activity and conversion from mild cognitive impairment to dementia
Authors: Kim Yeo Jin , Han Kyung-Do , Baek Min Seok , Cho Hanna , Lee Eun Joo , Lyoo Chul Hyoung
Published: September 2020

Background: Physical activity has been suggested to prevent the conversion of mild cognitive impairment (MCI) to dementia in patients. We investigated the association between the continuance and regularity of physical activity and the risk of developing dementia in patients with MCI.Methods: We analyzed 6-year followed up data for 247,149 individuals in the National Health Insurance Service (NHIS) cohort of Korea who were enrolled between January 1, 2009 and December 31, 2015. The patients were divided into four groups: those who did not engage in physical activity consistently (Never-PA group), those who initiated physical activity (Initiation-PA group), those who ceased physical activity (Withdrawal-PA group), and those who performed physical activity consistently (Maintenance-PA group). We also divided the patients into two groups: those who engaged in physical activity irregularly (Irregular-PA) and those who undertook physical activity regularly (Regular-PA).Results: When the risk for the Never-PA group was set as the benchmark (ref=1), the Maintenance-PA group had the lowest incidence of dementia of the Alzheimer type (DAT) compared to the other groups (HR= 0.82, 95% CI 0.79-0.86). The DAT risk of Initiation-PA group (HR = 0.89, 95% CI 0.85-0.93) was lower than the Never-PA group. In addition, compared to the Irregular-PA group, the Regular-PA group had a 15% reduced risk for developing DAT.Conclusions: Although no causal inference could be made, continued regular physical activity in patients with MCI is associated with a protective effect against developing DAT. Moreover, ceasing physical activity could halt this protective effect.
SisterC: A novel 3C-technique to detect chromatin interactions between and along sister chromatids
Authors: Oomen Marlies E. , Hedger Adam K. , Watts Jonathan K. , Dekker Job
Published: September 2020

Current chromosome conformation capture techniques are not able to distinguish sister chromatids. Here we describe the protocol of SisterC1: a novel Hi-C technique that leverages BrdU incorporation and UV/Hoechst-induced single strand breaks to identify interactions along and between sister chromatids. By synchronizing cells, BrdU is incorporated only on the newly replicated strand, which distinguishes the two sister chromatids2,3. This is followed by Hi-C4 of cells that can be arrested in different stages of the cell cycle, e.g. in mitosis. Before final amplification of the Hi-C library, strands containing BrdU are specifically depleted by UV/Hoechst treatment. SisterC libraries are then sequenced using 50bp paired end reads, followed by mapping using standard Hi-C processing tools. Interactions can then be assigned as inter- or intra-sister interactions based on read orientation.
KIAA0101 and UbcH10 interact to regulate non-small cell lung cancer cell proliferation by disrupting the function of the spindle assembly checkpoint
Authors: Lei Han , Wang Kun , Jiang Tongying , Lu Jingjing , Dong Xue , Wang Feilong , Li Qiang , Zhao Liming
Published: September 2020

Background: Chromosome mis-segregation caused by spindle assembly checkpoint (SAC) dysfunction during mitosis is an important pathogenic factor in cancer, and modulating SAC function has emerged as a potential novel therapy for non-small cell lung cancer (NSCLC). UbcH10 is considered to be associated with SAC function and the pathological types and clinical grades of NSCLC. KIAA0101, which contains a highly conserved proliferating cell nuclear antigen (PCNA)-binding motif that is involved in DNA repair in cancer cells, plays an important role in the regulation of SAC function in NSCLC cells, and bioinformatics predictions showed that this regulatory role is related to UbcH10. We hypothesized KIAA0101 and UbcH10 interact to mediate SAC dysfunction and neoplastic transformation during the development of USCLC. Methods: NSCLC cell lines were used to investigate the spatial-temporal correlation between UbcH10 and KIAA0101 expression and the downstream effects of modulating their expression were evaluated. Further immunoprecipitation assays were used to investigate the possible mechanism underlying the correlation between UbcH10 and KIAA0101. Eventually, the effect of modulating UbcH10 and KIAA010 on tumor growth and its possible mechanisms were explored through in vivo tumor-bearing models.Results: In this study, we demonstrated that both UbcH10 and KIAA0101 were upregulated in NSCLC tissues and cells and that their expression levels were correlated in a spatial and temporal manner. Importantly, UbcH10 and KIAA0101 coordinated to mediate the premature degradation of various SAC components to cause further SAC dysfunction and neoplastic proliferation. Moreover, tumor growth in vivo was significantly inhibited by silencing UbcH10 and KIAA0101 expression. Conclusions: KIAA0101 and UbcH10 interact to cause SAC dysfunction, chromosomal instability and malignant proliferation in NSCLC, suggesting that UbcH10 and KIAA0101 are potential therapeutic targets for the treatment of NSCLC by ameliorating SAC function.
Inducing multiple antibodies to treat squamous cell esophageal carcinoma
Authors: Hoshino Isamu , Nabeya Yoshihiro , Takiguchi Nobuhiro , Gunji Hisashi , Ishige Fumitaka , Iwatate Yosuke , Kuwajima Akiko , Shiratori Fumiaki , Okada Rei , Shimada Hideaki
Published: September 2020

Background: The positive response and the clinical usefulness of 14 serum antibodies in patients with esophageal squamous cell carcinoma (ESCC) were examined in this study. The Cancer Genome Atlas (TCGA) was used to investigate the frequency of gene expressions, mutations, and amplification of these 14 antigens and also the possible effects of antibody induction. Methods: Blood serum derived from 85 patients with ESCC was collected and analyzed for the 14 antibodies using ELISA. The prognosis between positive and negative antibodies were then compared. The antibody panel included galectin1, HCA25a, HCC-22-5, and HSP70. Results: Patient serum was positive for all antibodies, except VEGF, with the positive rates ranging from 1.18% to 10.59%. Positive rates for galectin1, HCA25a, HCC-22-5, and HSP70 were >10%. TCGA data revealed that all antigen-related genes had little or no mutation or amplification, and hence an increase in gene expression affected antibody induction. The positive results from the panel accounted for the positive rate comparable to the combination of CEA and SCC. No significant association was observed between the presence of antibodies and disease prognosis. Conclusions: The detection rates of galectin1, HCA25a, HCC-22-5, and HSP70 were 10% higher in patients with ESCC. Gene overexpression may be involved in such antibody production. These four antibodies were applied as a panel in comparison with conventional tumor markers. Moreover, it was confirmed that the combination of this panel and the conventional tumor markers significantly improved the positive rate.
A one-year cohort study of complications, continuation, and failure rates of postpartum TCu380A in Tanzania
Authors: Rwegoshora France John , Muganyizi Projestine Selestine , Kimario Grasiana Festus , Paul Ponsian Patrick , Makins Anita
Published: September 2020

Background Less than 1% of married women in Tanzania use an Intrauterine Contraceptive Device (IUD) for contraception. An initiative by the International Federation of Gynecology and Obstetrics (FIGO) has been in progress since 2015 resulting in escalated method uptake in implementing hospitals. This study investigates failure rate, complications, and risk factors for one-year continuation of TCu380A IUD when used for immediate postpartum contraception under the initiative in Tanzania Methodology A prospective cohort study of women who had TCu380A insertion within 48 hours of delivery in 6 hospitals in Tanzania between 1st December 2017 and 18th April 2018 was conducted. Face to face post insertion interviews were made with 1114 clients before discharge and later through phone calls up to the beginning of 13th month postpartum. Postpartum Intrauterine Device (PPIUD) continuation status, complications, duration of time they stayed with the IUD and the currently used method if PPIUD was discontinued were enquired. The outcome variable was PPIUD continuation at one year of IUD insertion. Data were analyzed using Statistical Product and Service Solutions software (SPSS) for Windows version 20 (IBM SPSS Statistics, Chicago, IL, USA). Results In total 511(45.8%)clients had consented and availed to complete the one-year follow-up. Out of these, 440 still had IUD, giving a one-year continuation rate of 86.1%. Most (63%) IUD discontinuations occurred in the period between 7th week and 6 months of insertion. One-year method expulsion rate was 2.1%. There was one reported pregnancy that gives a method failure rate of about 2 per 1000. The independent risk factors in favor of method continuation at one year were absence of medical or social problem, being a youth (16-24 years), and delivery by Cesarean section. Conclusions The continuation rate when CuT380A is used for immediate postpartum contraception is high, with low complication and failure rates. Some medical and social factors are important for method continuation, hence the need to consider in training, counselling and advocacy.
Increased E2F2 predicts poor prognosis in patients with HCC based on TCGA data
Authors: Zeng Zhili , Cao Zebiao , Tang Ying
Published: September 2020

Background: The E2F family of transcription factor 2 (E2F2) plays an important role in the development and progression of various tumors, but its association with hepatocellular carcinoma (HCC) remains unknown. Our study aimed to investigate the role and clinical significance of E2F2 in HCC.Methods: HCC raw data were extracted from The Cancer Genome Atlas (TCGA). Wilcoxon signed-rank test, Kruskal-Wallis test and logistic regression were applied to analyze the relationship between the expression of E2F2 and clinicopathologic characteristics. Cox regression and Kaplan-Meier were employed to evaluate the correlation between clinicopathologic features and survival. The biological function of E2F2 was annotated by Gene Set Enrichment Analysis (GSEA).Results: The expression of E2F2 was increased in HCC samples. The expression of elevated E2F2 in HCC samples was prominently correlated with histologic grade (OR =2.62 for G3-4 vs. G1-2, p=1.80E-05), clinical stage (OR =1.74 for III-IV vs. I-II, p=0.03), T (OR =1.64 for T3-4 vs.T1-2, p=0.04), tumor status (OR =1.88 for with tumor vs. tumor free, p= 3.79E-03), plasma alpha fetoprotein (AFP) value (OR =3.18 for AFP≥400 vs AFP＜20, p= 2.16E-04; OR=2.50 for 20≤AFP＜400 vs AFP＜20, p=2.56E-03). Increased E2F2 had an unfavorable OS (p=7.468e−05), PFI (p=3.183e−05), DFI (p=0.001), DSS (p=4.172e−05). Elevated E2F2 was independently bound up with OS (p =0.004, hazard ratio [HR]= 2.4 (95% CI [1.3-4.2])), DFI (P =0.029, hazard ratio [HR] = 2.0 (95% CI [1.1-3.7])) and PFI (P =0.005, hazard ratio [HR] = 2.2 (95% CI [1.3-3.9])). GSEA disclosed that cell circle, RNA degradation, pyrimidine metabolism, base excision repair, aminoacyl tRNA biosynthesis, DNA replication, p53 signaling pathway, nucleotide excision repair, ubiquitin-mediated proteolysis, citrate cycle TCA cycle were notably enriched in E2F2 high expression phenotype.Conclusions: Elevated E2F2 can be a promising independent prognostic biomarker and therapeutic target for HCC. Additionally, cell cycle, pyrimidine metabolism, DNA replication, p53 signaling pathway, ubiquitin-mediated proteolysis, the citrate cycle TCA cycle may be the key pathway by which E2F2 participates in the initial and progression of HCC.
Data Processing Scheme Based on Blockchain
Authors: Fu Jinhua , Xu Mixue , Huang Yongzhong , Si Xueming , Yuan Chao
Published: September 2020

In the white paper written on Bitcoin, a chain of blocks was proposed by Satoshi Nakamoto. Since then, blockchain has been rapidly developed. Blockchain is not only limited to the field of cryptocurrency but also has been extensively applied to the Internet of Things (IoT), supply chain finance, electronic evidence storage, data sharing, and e-government fields. Both the public chain and the alliance chain have been extensively developed. In the data processing field, blockchain has a particularly good application potential. The Square Kilometre Array (SKA) is a proposal consisting of a joint venture of more than ten countries, resulting in the world’s largest synthetic aperture radio telescope. In the SKA, the processing scale of the data is large, and it consists of several data processing nodes. The data will be processed in the cloud computing mode. Taking the SKA under consideration, this report proposes a data processing scheme based on blockchain for the anti-counterfeiting, anti-tampering and traceability of data. Furthermore, the authenticity and integrity of the data are assured. The primary aspects include data distribution, data operation and data sharing, which correspond to the data reception, data algorithm processing and result sharing of data operation in the SKA. With this process, the integrity, reliability and authenticity of the data are guaranteed. Additionally, smart contracts, homomorphic hashing, secure containers, aggregate signatures and one-way encrypted channels are implemented to ensure the intelligence, security and high performance of the process.
Adaptation and preliminary psychometric properties of three self-stigma outcome measures for people living with dementia
Authors: MSc Jemini Bhatt BSc , Stoner Charlotte R , Scior Katrina , Charlesworth Georgina
Published: September 2020

Background: A diagnosis of dementia presents individuals with both social and psychological challenges but research on self-stigma in dementia has been largely confined to qualitative approaches due to a lack of robust outcome measures that assess change. The Stigma Impact Scale (SIS) is the most commonly used measure of self-stigma in dementia but its suitability as a tool to assess change in a diverse UK population is unclear. Thus, the aim of this study was to identify, adapt and evaluate the acceptability and preliminary psychometric properties of self-stigma measures for people with dementia for use as measures of change. Method: A 4-step sequential design of identifying, selecting, adapting and testing psychometric measures as follows: 1) identification of stigma outcome measures through reviewing anti-stigma intervention literature, 2) selection of candidate measures through quality assessment (Terwee criteria) and expert consultation, 3) adaptation for UK dementia population (Stewart and colleagues Modification Framework) 4) testing of adapted measures in people living with dementia (N=40) to establish acceptability and preliminary reproducibility (test retest), criterion (concurrent with SIS) and construct (negative convergence with Rosenberg self-esteem scale) validity. Results: Seven measures were identified from the review, but most were poor quality (Terwee range: 0-4). Three measures were selected for modification: Stigma Stress Scale; Secrecy subscale of the Stigma Coping Orientation Scale; Disclosure Related Distress Scale. Internal consistency and test-retest reliability were acceptable (.866≤α≤ .938; ICC .721-.774), except for the Stigma Stress Scale (α= .643) for which the component subscales (perceived harm, ability to cope) had stronger psychometric properties. Concurrent validity with the SIS was not established (r<.7) although there were significant correlations between total SIS and perceived harm (r=.587) and between internalized shame and secrecy (r=.488). Relationships with self-esteem were in the hypothesized direction for all scales and subscales indicating convergent validity.Conclusion: Stigma scales from mental health are not readily adapted for use with people with dementia. However there is preliminary evidence for the acceptability, reliability and validity of measures of perceived harm, secrecy and stigma impact. Further conceptual and psychometric development is required.
CXCL1 contributes to IL-6 expression in osteoarthritis and rheumatoid arthritis synovial fibroblasts by CXCR2, c-Raf, MAPK and AP-1 pathway
Authors: Hou Sheng-Mou , Chen PoChun , Lin Chieh-Mo , Fang Mei-Ling , Chi Miao-Ching , Liu Ju-Fang
Published: September 2020

Background: Osteoarthritis (OA) and rheumatoid arthritis (RA) are common joint disorders that are considered to be different diseases due to their unique molecular mechanisms and pathogenesis. Chemokines and their corresponding receptors have been well-characterized in RA progression, but less so in OA pathogenesis.Methods: The human primary synovial fibroblasts (SFs) were obtained from human OA and RA tissue samples. The Western blot and qPCR were performed to analyze expression levels of CXCL1, as well as CXCL-promoted IL-6 expression in both OASFs and RASFs. The signal cascades that mediate the CXCL1-promoted IL-6 expression were identified by using chemical inhibitors, siRNAs and shRNAs.Results: Here, we found that both diseases feature elevated levels of CXCL1 and interleukin (IL)-6, an important proinflammatory cytokine that participates in OA and RA pathogenesis. In OASFs and RASFs, CXCL1 promoted IL-6 expression in a dose- and time-dependent manner. In OASFs and RASFs overexpressing CXCL1 or transduced with shRNA plasmid, IL-6 expression was markedly upregulated. CXCR2, c-Raf and MAPKs was found to regulate CXCL1-induced IL-6 expression in OASFs and RASFs. Finally, CXCL1 triggered the transcriptional activities of c-Jun (which regulates the expression of proinflammatory proteins) in OASFs and RASFs.Conclusions: Our present work suggests that the CXCL1/CXCR2 axis helps to orchestrate inflammatory responses in OA and RA SFs.
Phase II study of sequential S-1 and cyclophosphamide therapy in patients with metastatic breast cancer
Authors: Yanai Keiko , Fujii Takaaki , Horiguchi Jun , Nakazawa Yuko , Kurozumi Sasagu , Obayashi Sayaka , Yajima Reina , Shirabe Ken
Published: September 2020

Background: S-1 and cyclophosphamide (CPA) can be given orally, and their combination may have great potential for treating metastatic breast cancer (MBC). A phase I study of sequential S-1 and CPA therapy was conducted in patients with MBC; the recommended doses that were determined for this regimen were 80 mg/m2/day for S-1 and 100 mg/m2/day for CPA. We then conducted a phase II study of this oral S-1 and CPA regimen. Methods: This was a single-arm, open-label, single-center prospective phase II study to evaluate the efficacy of a sequential S-1 and CPA regimen for MBC. S-1 was administered orally 2´/day for 14 consecutive days, and then CPA was administered orally 2´/day for 14 consecutive days in a repeating 4-week cycle (S-1 for 2 weeks, CPA for 2 weeks). The primary endpoint was the overall response rate (ORR). Secondary endpoints included the overall survival (OS), progression-free survival (PFS), clinical benefit rate (CBR) and safety. Results: Thirty-six patients were enrolled in this study. The overall response was complete response in 0 (0%), partial response in 12 (33.3%), stable disease in 12 (33.3%), and progressive disease in 11 (30.1%) patients. The ORR was 33.3% (12/36). The CBR was 66.7% (24/36). The median PFS was 9.5 months (95%CI: 7.8–12.6 months). The median OS was 20.2 months (95%CI: 15.0–25.4 months) Grade 3/4 adverse events included leukopenia in seven patients (19.4%). Dose reductions because of adverse events occurred in 12 patients (33.3%). There was no treatment-related mortality. Conclusion: The combination of sequential therapy with S-1 and CPA was tolerable and had efficacy with good disease control. Sequential therapy with S-1 and CPA may be a feasible new treatment option for patients with MBC, however, further study is warranted to explore the efficacy of this therapy.Trial registration: JRCT, JRCTs 031180296. Registered 2 December 2019 – Retrospectively registered, https://jrct.hiph.go.jp/latest-detail.jrcts031180296