bluuebunny/crossref_metadata_2025 huggingface dataset:
Validation of Corporate Probability of Default Models Considering Alternative Use Cases
Authors: Jacobs Michael
Published: December 2021

In this study, we consider the construction of through-the-cycle (“TTC”) PD models designed for credit underwriting uses and point-in-time (“PIT”) PD models suitable for early warning uses, considering which validation elements should be emphasized in each case. We build PD models using a long history of large corporate firms sourced from Moody’s, with a large number of financial, equity market and macroeconomic variables as candidate explanatory variables. We construct a Merton model-style distance-to-default (“DTD”) measure and build hybrid structural reduced-form models to compare with the financial ratio and macroeconomic variable-only models. In the hybrid models, the financial and macroeconomic explanatory variables still enter significantly and improve the predictive accuracy of the TTC models, which generally lag behind the PIT models in that performance measure. We conclude that care must be taken to judiciously choose the manner in which we validate TTC vs. PIT models, as criteria may be rather different and be apart from standards such as discriminatory power. This study contributes to the literature by providing expert guidance to credit risk modeling, model validation and supervisory practitioners in controlling the model risk associated with such modeling efforts.
Plasticity of Proinflammatory Macrophages Depends on Their Polarization Stage during Human MSC Immunomodulation—An In Vitro Study Using THP-1 and Human Primary Macrophages
Authors: Ißleib Constantin , Kurz Susanne , Scholl Samuel , Amberg Bettina , Spohn Juliane
Published: December 2021

Human mesenchymal stem cells (hMSCs) are well-known for their immunomodulatory potential. In recent clinical trials and in vivo studies, hMSCs were used as therapeutic measures to dampen inflammation. In this context, their effect on macrophages in vivo has been described to induce a phenotype change shifting from a proinflammatory to an anti-inflammatory environment. Despite several in vitro studies that investigated the potential of hMSCs to inhibit the polarization of macrophages into the proinflammatory M1 subtype, it is still unclear if hMSCs affect polarized M1 macrophages or if they control the environment by inhibiting the M1 polarization of unpolarized macrophages. Here, a comparative in vitro investigation of hMSC immunomodulation via soluble factors concerning the influence on the polarization of macrophages to M1 and on polarized M1 macrophages is presented. Human primary monocyte-derived macrophages (hMDMs) as well as THP-1 cells were used for this investigation. The macrophage subtype was analyzed by gene expression as well as cytokine secretion. hMSCs affected cytokine secretion of polarizing macrophages, while changes in gene expression were evident in polarized M1 macrophages. These effects were observed in THP-1 and hMDM macrophages. In conclusion, we suggest that hMSCs implement their immunomodulatory effects on polarizing and polarized macrophages in different ways.
Geographic Location Determines Differentially Methylated Gene Expressions in Autoimmune Diseases
Authors: Pers Jacques-Olivier , Bahane Hajar , Le Dantec Christelle , Foulquier Nathan , Alarcon-Riquelme Marta E. , Youinou Pierre ,   PRECISESADS Clinical Consortium
Published: December 2021

Further observations support the role of environmental factors in autoimmune diseases via the alteration of the epigenetic machinery. In this context, ultraviolet light, smoking, chemicals, and psychological stress have been described as likely examples of this phenomenon. For this study, we took advantage of the PRECISESADS IMI project, which gathered joint data from 2500 individuals with systemic autoimmune diseases, including systemic lupus erythematosus (SLE), systemic sclerosis (SSc), primary Sjögren’s syndrome (pSS), rheumatoid arthritis (RA), primary antiphospholipid syndrome (PAPS), and mixed connective tissue disease (MCTD), and aimed to determine such epigenetic modifications in SLE, SSc, pSS, and RA patients. Here, we performed a set of measures in several countries from the north and south of Europe. We observed that autoimmune patients from the north of Europe presented a higher hypomethylated profile associated with an increased gene expression than patients from the south. These genes were associated with interferon (IFN) pathways, immunity, and the pathways associated with cellular metabolism. Since the IFN scores were increased in this population, these patients presented a more inflammatory profile. To conclude, the geographical location of patients with autoimmune diseases has an impact on DNA methylation, RNA expression, and immunological profiles.
Functionalized Tris(anilido)triazacyclononanes as Hexadentate Ligands for the Encapsulation of U(III), U(IV) and La(III) Cations
Authors: Formanuik Alasdair , Ortu Fabrizio , Vitorica-Yrezabal Iñigo J. , Tuna Floriana , McInnes Eric J. L. , Natrajan Louise S. , Mills David P.
Published: December 2021

Tripodal multidentate ligands have become increasingly popular in f-element chemistry for stabilizing unusual bonding motifs and supporting small molecule activation processes. The steric and electronic effects of ligand donor atom substituents have proved crucial in both of these applications. In this study we functionalized the previously reported tris-anilide ligand {tacn(SiMe2NPh)3} (tacn = 1,3,7-triazacyclononane) to incorporate substituted aromatic rings, with the aim of modifying f-element complex solubility and ligand steric effects. We report the synthesis of two proligands, {tacn(SiMe2NHAr)3} (Ar = C6H3Me2-3,5 or C6H4Me-4), and their respective group 1 transfer agents—{tacn(SiMe2NKAr)3}, M(III) complexes [M{tacn(SiMe2NAr)3}] for M = La and U, and U(IV) complexes [M{tacn(SiMe2NAr)3}(Cl)]. These compounds were characterized by multinuclear NMR and FTIR spectroscopy and elemental analysis. The paramagnetic uranium complexes were also characterized by solid state magnetic measurements and UV/Vis/NIR spectroscopy. U(III) complexes were additionally studied by EPR spectroscopy. The solid state structures of all f-block complexes were authenticated by single-crystal X-ray diffraction (XRD), together with a minor byproduct [U{tacn(SiMe2NC6H4Me-4)3}(I)]. Comparisons of the characterization data of our f-element complexes with similar literature examples containing the {tacn(SiMe2NPh)3} ligand set showed minor changes in physicochemical properties resulting from the different aromatic ring substitution patterns we investigated.
Preliminary Design of the Support Structure for a Rotating Carbon-Ion Transfer Line for Medical Applications
Authors: Perini Diego , Dassa Luca , Piacentini Luca , Uberti Stefano
Published: December 2021

The development of new bent superconducting magnets together with the optimization of the support structure open the way to a considerable reduction in the weight and complexity of rotating gantries for medical applications. The magnets, which define the transfer line to deliver carbon ions to the patients from different angles, are supported by a rotating structure that should be as rigid and as lightweight as possible. Relative displacements of the magnets due to deformations cause incorrect beam position and consequent errors in hitting the target tissues. This paper describes a possible rotating structure which is considerably lighter than the previous designs. A method to compensate part of the deformation by complementary rotations of the driving motor is proposed. The influence of the construction tolerances and deformations of the supports is also analyzed and alignment and adjustment possibilities are discussed.
The Impact of Cattaneo–Christov Double Diffusion on Oldroyd-B Fluid Flow over a Stretching Sheet with Thermophoretic Particle Deposition and Relaxation Chemical Reaction
Authors: Shankaralingappa Bheemasandra M. , Prasannakumara Ballajja C. , Gireesha Bijjanal J. , Sarris Ioannis E.
Published: December 2021

The current study focuses on the characteristics of flow, heat, and mass transfer in the context of their applications. There has been a lot of interest in the use of non-Newtonian fluids in biological and technical disciplines. Having such a substantial interest in non-Newtonian fluids, our goal is to explore the flow of Oldroyd-B liquid over a stretching sheet by considering Cattaneo–Christov double diffusion and heat source/sink. Furthermore, the relaxation chemical reaction and thermophoretic particle deposition are considered in the modelling. The equations that represent the indicated flow are changed to ordinary differential equations (ODEs) by choosing relevant similarity variables. The reduced equations are solved using the Runge–Kutta–Fehlberg fourth–fifth order technique (RKF-45) and a shooting scheme. Physical descriptions are strategized and argued using graphical representations to provide a clear understanding of the behaviour of dimensionless parameters on dimensionless velocity, concentration, and temperature profiles. The results reveal that the rising values of the rotation parameter lead to a decline in the fluid velocity. The rise in values of relaxation time parameters of temperature and concentration decreases the thermal and concentration profiles, respectively. The increase in values of the heat source/sink parameter advances the thermal profile. The rise in values of the thermophoretic and chemical reaction rate parameters declines the concentration profile.
Metabolic Syndrome and Its Components in Patients with COVID-19: Severe Acute Respiratory Syndrome (SARS) and Mortality. A Systematic Review and Meta-Analysis
Authors: Rico-Martín Sergio , Calderón-García Julián F. , Basilio-Fernández Belinda , Clavijo-Chamorro María Zoraida , Sánchez Muñoz-Torrero Juan F.
Published: December 2021

Recent meta-analysis studies have reported that metabolic comorbidities such as diabetes, obesity, dyslipidaemia and hypertension are associated with higher risk of severe acute respiratory syndrome (SARS) and mortality in patients with COVID-19. This meta-analysis aims to investigate the relationship between metabolic syndrome (MetS) and its components with SARS and mortality in COVID-19 patients. Methods: A systematic search was conducted in the several databases up until 1 September 2021. Primary observational longitudinal studies published in peer review journals were selected. Two independent reviewers performed title and abstract screening, extracted data and assessed the risk of bias using the Newcastle–Ottawa Scale. Results: The random effects meta-analysis showed that MetS was significantly associated with SARS with a pooled OR (95% CI) of 3.21 (2.88–3.58) and mortality with a pooled OR (95% CI) of 2.32 (1.16–4.63). According to SARS, the pooled OR for MetS was 2.19 (1.71–2.67), p < 0.001; significantly higher than the hypertension component. With regard to mortality, although the pooled OR for MetS was greater than for its individual components, no significant differences were observed. Conclusions: this meta-analysis of cohort studies, showed that MetS is better associated to SARS and mortality in COVID-19 patients than its individual components.
Acute Cardiorespiratory Responses to Different Exercise Modalities in Chronic Heart Failure Patients—A Pilot Study
Authors: Karatzanos Eleftherios , Ferentinos Panagiotis , Mitsiou Georgios , Dimopoulos Stavros , Ntalianis Argyrios , Nanas Serafeim
Published: December 2021

The purpose of this study was to compare the acute cardiorespiratory responses and time spent above different %VO2peak intensities between three “iso-work” protocols: (a) a high intensity interval training protocol (HIIT), (b) a higher intensity continuous protocol (CON70) and (c) a lower intensity continuous protocol (CON50) in patients with chronic heart failure (CHF). Ten male CHF patients (aged 55.1 ± 16.2 years) performed in separate days a single session of a HIIT protocol consisted of 4 sets × 4 min cycling at 80% VO2peak with 3 min of recovery at 50% VO2peak, a CON70 protocol corresponding to 70% VO2peak and a CON50 protocol corresponding to 50% VO2peak. Cardiopulmonary data were collected by an online gas analysis system. The HIIT and CON70 elicited higher cardiorespiratory responses compared to CON50 with no differences between them (p > 0.05). In HIIT and CON70, patients exercised longer at >80% and >90% VO2peak. The completion rate was 100% for the three protocols. Not any adverse events were observed in either protocol. Both HIIT and CON70 elicited a stronger physiological stimulus and required shorter time than CON50. Both HIIT and CON70 also induced comparable hemodynamic responses and ventilatory demand.
Long-Term Survival in Patients with Post-Operative Atrial Fibrillation after Cardiac Surgery: Analysis from a Prospective Cohort Study
Authors: Marazzato Jacopo , Masnaghetti Sergio , De Ponti Roberto , Verdecchia Paolo , Blasi Federico , Ferrarese Sandro , Trapasso Monica , Spanevello Antonio , Angeli Fabio
Published: December 2021

Background: Post-operative (POP) atrial fibrillation (AF) is frequent in patients who undergo cardiac surgery. However, its prognostic impact in the long term remains unclear. Methods: We followed 1386 patients who underwent cardiac surgery for an average of 10 ± 3 years. According to clinical history of AF before and after surgery, four subgroups were identified: (1) patients with no history of AF and without episodes of AF during the first 30 days after surgery (control or Group 1, n = 726), (2) patients with no history of AF before surgery in whom new-onset POP AF was detected during the first 30 days after surgery (new-onset POP AF or Group 2, n = 452), (3) patients with a history of paroxysmal/persistent AF before cardiac surgery (Group 3, n = 125, including 87 POP AF patients and 38 who did not develop POP AF), and (4) patients with permanent AF at the time of cardiac surgery (Group 4, n = 83). All-cause mortality was the primary outcome of the study. We tested the associations of potential determinants with all-cause mortality using univariable and multivariable statistical analyses. Results: Overall, 473 patients (34%) died during follow-up. After adjustment for multiple confounders, new-onset POP AF (hazard ratio (HR) = 1.31, 95% confidence interval (CI): 0.90–1.89; p = 0.1609), history of paroxysmal/persistent AF before cardiac surgery (HR = 1.33, 95% CI: 0.71–2.49; p = 0.3736), and permanent AF (Group 4) (HR = 1.55, 95% CI 0.82–2.95; p = 0.1803) were not associated with a significantly increased risk of mortality when compared with Group 1 (patients with no history of AF and without episodes of AF during the first 30 days after surgery). In new-onset POP AF patients, oral anticoagulation was not associated with mortality (HR = 1.13, 95% CI: 0.83–1.54; p = 0.4299). Conclusions: In this cohort of patients who underwent different types of heart surgery, POP AF was not associated with an increased risk of mortality. In this setting, the role of long-term anticoagulation remains unclear.
Modeling Correlation between Android Permissions Based on Threat and Protection Level Using Exploratory Factor Plane Analysis
Authors: Ashawa Moses , Morris Sarah
Published: December 2021

The evolution of mobile technology has increased correspondingly with the number of attacks on mobile devices. Malware attack on mobile devices is one of the top security challenges the mobile community faces daily. While malware classification and detection tools are being developed to fight malware infection, hackers keep deploying different infection strategies, including permissions usage. Among mobile platforms, Android is the most targeted by malware because of its open OS and popularity. Permissions is one of the major security techniques used by Android and other mobile platforms to control device resources and enhance access control. In this study, we used the t-Distribution stochastic neighbor embedding (t-SNE) and Self-Organizing Map techniques to produce a visualization method using exploratory factor plane analysis to visualize permissions correlation in Android applications. Two categories of datasets were used for this study: the benign and malicious datasets. Dataset was obtained from Contagio, VirusShare, VirusTotal, and Androzoo repositories. A total of 12,267 malicious and 10,837 benign applications with different categories were used. We demonstrate that our method can identify the correlation between permissions and classify Android applications based on their protection and threat level. Our results show that every permission has a threat level. This signifies those permissions with the same protection level have the same threat level.
Investigations on Structural and Optical Properties of Various Modifier Oxides (MO = ZnO, CdO, BaO, and PbO) Containing Bismuth Borate Lithium Glasses
Authors: Bhemarajam J. , Syam Prasad P. , Mohan Babu M. , Özcan Mutlu , Prasad M.
Published: December 2021

Bismuth based quaternary glasses with compositions BiBLM: 50Bi2O3–20B2O3–15Li2O–15MO (where MO = ZnO, CdO, BaO, and PbO) were processed by conventional melt quenching. The effectiveness of various modifier oxides on the optical and structural properties of the developed glasses was studied systematically by XRD, DSC, FTIR, Raman, and optical absorption (OA) measurements. The synthesized glass specimens were characterized by XRD and the patterns demonstrated an amorphous nature. The physical characteristics such as molar mass, density, and OPD values were found to increase with an increase in the molar mass of the modifier oxides, while there was a decrement in oxygen molar volume, thus resulting in decrement of complete molar volume of the prepared glasses. From DSC analysis, incorrigible reduction and enhancement of Tg and thermal stability among various modifier oxides in the glass network was noticed. Optical absorption data for glass specimens have confirmed the decrease in both direct and indirect optical band gap values among various modifier oxides incorporation. These investigations support the obtained Urbach energy (UE) and metallization criteria of synthesized glasses. The ionic characteristic for the glass specimens were confirmed by the values of electronic polarizability and electronegativity. The Raman and FT-IR spectra of the glass specimens displayed the existence of BiO3, BiO6, ZnO4, CdO4, BaO4, BO3, PbO4, and BO4 structural units within the glass matrix. These structural results can support the applications of as-developed glasses in the area of photonics.
Flexible and High Thermal Conductivity Composites Based on Graphite Nanoplates Paper Impregnated with Polydimethylsiloxane
Authors: Battegazzore Daniele , Fadda Erica , Fina Alberto
Published: December 2021

This paper deals with the design, preparation, and characterization of conductive and flexible nanopapers based on graphite nanoplates (GNP) and polydimethylsiloxane (PDMS). Highly porous GNP nanopapers were first prepared by filtration from a GNP suspension in a solvent. Subsequently, PDMS impregnation was carried out to obtain a composite material. By varying the concentration of the polymer solution and the deposition time, PDMS/GNP nanopapers were produced with a wide range of PDMS contents, porosities, and densities. Thermal diffusivity of the composite films (both in-plane and cross-plane) were measured and correlated with the structure of the nanopapers. Selected formulations were investigated in detail for their physical, thermal, and mechanical properties, exhibiting high flexibility and resistance to more than 50 repeated bendings, stiffness of up to 1.3 MPa, and thermal conductivity of up to 25 W/m∙K. Based on the properties obtained, the materials presented in this paper may find applications in modern lightweight and flexible electronic devices.
Rubberized Geopolymer Composites: Value-Added Applications
Authors: Luhar Ismail , Luhar Salmabanu
Published: December 2021

The discovery of an innovative class of inorganic polymers has brought forth a revolution in the history of construction technology. Now, no energy-intensive reactions at elevated temperatures are essential, as found in the case of contemporary cement production. In addition to their attributes of low energy and a mitigated carbon footprint, geopolymeric composites can incorporate diversely originated and profound wastes in their manufacturing. As of today, profoundly accessible landfills of rubber tyre waste negatively impact the environment, water, and soil, with many health hazards. Their nonbiodegradable complex chemical structure supports recycling, and toxic gases are emitted by burning them, leading to aesthetic issues. These, altogether, create great concern for well-thought-out disposal methods. One of the achievable solutions is processing this waste into alternative aggregates to thus generate increased economic value whilst reducing primary aggregate consumption through the incorporation of these vast automobile solid wastes in the manufacturing of geopolymer construction composites, e.g., binders, mortar, concrete, etc., produced through the process of geopolymerization as a replacement for natural aggregates, providing relief to the crisis of the degradation of restricted natural aggregate resources. Currently, tyre rubber is one of the most outstanding materials, extensively employed in scores of engineering applications. This manuscript presents a state-of-the-art review of value-added applications in the context of rubberized geopolymer building composites and a review of past investigations. More significantly, this paper reviews rubberized geopolymer composites for their value-added applications.
Controllable Synthesis of Graphene-Encapsulated NiFe Nanofiber for Oxygen Evolution Reaction Application
Authors: Li Mengyang , Rong Jiayi , Guo Ning , Chen Susu , Gao Meiqi , Cao Feng , Li Guoqing
Published: December 2021

Carbon-Encapsulated NiFe Nanofiber NixFey@C-CNFs have been demonstrated to be promising candidates to replace conventional nobel metals-based catalysts for oxygen evolution reaction. Here, we developed a facile method of electrospinning and high temperature carbonization to synthesize NixFey@C-CNFs catalysts. It is proved that Ni3Fe7@C-CNFs exhibited low overpotential (245 mV) and excellent stability in alkaline electrolyte for OER. This work provides a good platform for the synthesis and design of graphene-encapsulated alloy catalysts.
Injection Molding of Wood-Filled Thermoplastic Polyurethane
Authors: Moritzer Elmar , Richters Maximilian
Published: December 2021

Wood fiber reinforcement of plastics is almost limited to polypropylene, polyethylene, polyvinyl chloride and polystyrene. Wood fiber reinforcement of thermoplastic polyurethanes (TPU) is a new research field and paltry studied scientifically. Wood fiber reinforcement can carry out synergistic effects between sustainability, material or product price reduction, improved mechanical properties at high elongation, and brilliant appearance and haptics. In order to evaluate to what extent the improvement of mechanical properties depend on material-specific parameters (fiber type, fiber content) and on process-specific parameters (holding pressure, temperature control and injection speed), differently filled compounds were injection molded according to a partial factorial test plan and subjected to characterizing test procedures (tensile test, Shore hardness and notched impact test). Tensile strength showed significant dependence on barrel temperature, fiber type and interaction between holding pressure and barrel temperature in the region of interest. Young’s modulus can be influenced by fiber content but not by fiber type. Notched impact strength showed a significant influence of cylinder temperature, fiber content, fiber type and the interaction between cylinder temperature and fiber content in the region of interest. Shore hardness is related to fiber content and the interaction between mold temperature and injection flow rate. Our results show not only that wood-filled TPU can be processed very well by injection molding, but also that the mechanical properties depend significantly on temperature control in the injection-molding process. Moreover, considering the significant reinforcing effect of the wood fibers, a good fiber-matrix adhesion can be assumed.
Eccentric Exercise: Adaptations and Applications for Health and Performance
Authors: Harris-Love Michael O. , Gollie Jared M. , Keogh Justin W. L.
Published: December 2021

The goals of this narrative review are to provide a brief overview of the muscle and tendon adaptations to eccentric resistance exercise and address the applications of this form of training to aid rehabilitative interventions and enhance sports performance. This work is centered on the author contributions to the Special Issue entitled “Eccentric Exercise: Adaptations and Applications for Health and Performance”. The major themes from the contributing authors include the need to place greater attention on eccentric exercise mode selection based on training goals and individual fitness level, optimal approaches to implementing eccentric resistance exercise for therapeutic purposes, factors that affect the use of eccentric exercise across the lifespan, and general recommendations to integrate eccentric exercise in athletic training regimens. The authors propose that movement velocity and the absorption or recovery of kinetic energy are critical components of eccentric exercise programming. Regarding the therapeutic use of eccentric resistance training, patient-level factors regarding condition severity, fitness level, and stage of rehabilitation should govern the plan of care. In athletic populations, use of eccentric exercise may improve movement competency and promote improved safety and performance of sport-specific tasks. Eccentric resistance training is a viable option for youth, young adults, and older adults when the exercise prescription appropriately addresses program goals, exercise tolerability, and compliance. Despite the benefits of eccentric exercise, several key questions remain unanswered regarding its application underscoring the need for further investigation.
A Rate Theory Model of Radiation-Induced Swelling in an Austenitic Stainless Steel
Authors: Griffiths Malcolm , Ramos-Nervi Juan , Greenwood Larry
Published: December 2021

Many rate theory models of cavity (void) swelling have been published over the past 50 years, all having the same, or similar, structures. A rigorous validation of the models has not been possible because of the dearth of information concerning the microstructures that correspond with the swelling data. Whereas the lack of microstructure information is still an issue for historical swelling data, in the past 10–20 years data have been published on the evolution of the microstructure (point defect yields from collision cascades, cavity number densities, and dislocation densities/yield strengths) allowing certain gaps in information to be filled when considering historic swelling data. With reasonable estimates of key microstructure parameters, a standard rate theory model can be applied, and the model parameter space explored, in connection with historical swelling data. By using published data on: (i) yield strength as a function of dose and temperature (to establish an empirical expression for dislocation density evolution); (ii) cavity number densities as a function of temperature; and (iii) freely migrating defect (FMD) production as a function of primary knock-on atom (PKA) spectrum, the necessary parameter and microstructure inputs that were previously unknown can be used in model development. This paper describes a rate-theory model for void swelling of 316 stainless steel irradiated in the EBR-2 reactor as a function of irradiation temperature and neutron dose.
Novel Pathogenic Mucorales Identified Using the Silkworm Infection Model
Authors: Panthee Suresh , Hamamoto Hiroshi , Nishiyama Yayoi , Paudel Atmika , Sekimizu Kazuhisa
Published: December 2021

Mucormycosis, a rare but highly fatal infection, is caused by fungi of the order Mucorales. Due to their ubiquitous nature, reduced susceptibility to antifungals, acid tolerance, and ability to infect immunocompromised patients through rapid dissemination, these fungi have been frequently reported to infect the COVID-19 patients. In order to develop strategies to overcome mucormycosis, it is essential to understand and identify novel Mucorales present in the environment. In this study, we report the identification of four novel pathogenic Mucorales using the silkworm (Bombyx mori) model. The strains’ phylogeny was analyzed using the genome sequence of the large subunit ribosomal ribonucleic acid (LSU rRNA) and the internal transcribed spacer (ITS) region, where strains 1-3, 5-3, and S286-1101 claded with Mucor orantomantidis, and strain 827-14 claded with Backusella lamprospora. All the strains had a cold-sensitive phenotype with their inability to grow prominently at 4 °C. Mucor sp. 1-3 and 5-3 were characterized by their filamentous and yeast-like growth under aerobic and anaerobic conditions, respectively. The yeast colonies of Mucor sp. 5-3 had multipolar budding cells often observed with cleaved cell surfaces under a scanning electron microscope. We further found that these strains were able to kill immunocompromised mice suggesting their pathogenicity to mammals. Our study established an invertebrate model-based screening system to identify novel pathogenic Mucorales from the natural environment and provided a clue towards the rapid increase in COVID-19 related mucormycosis.
Expression and Purification along with Evaluation of Serological Response and Diagnostic Potential of Recombinant Sap2 Protein from C. parapsilosis for Use in Systemic Candidiasis
Authors: Shukla Manisha , Chandley Pankaj , Kaur Harsimran , Ghosh Anup K. , Rudramurthy Shivaprakash M. , Rohatgi Soma
Published: December 2021

Systemic candidiasis is the fourth most common bloodstream infection in ICU patients worldwide. Although C. albicans is a predominant species causing systemic candidiasis, infections caused by non-albicans Candida (NAC) species are increasingly becoming more prevalent globally along with the emergence of drug resistance. The diagnosis of systemic candidiasis is difficult due to the absence of significant clinical symptoms in patients. We investigated the diagnostic potential of recombinant secreted aspartyl proteinase 2 (rSap2) from C. parapsilosis for the detection of Candida infection. The rSap2 protein was successfully cloned, expressed and purified using Ni-NTA chromatography under denaturing conditions using an E. coli-based prokaryotic expression system, and refolded using a multi-step dialysis procedure. Structural analysis by CD and FTIR spectroscopy revealed the refolded protein to be in its near native conformation. Immunogenicity analysis demonstrated the rSap2 protein to be highly immunogenic as evident from significantly high titers of Sap2-specific antibodies in antigen immunized Balb/c mice, compared to sham-immunized controls. The diagnostic potential of rSap2 protein was evaluated using immunoblotting and ELISA assays using proven candidiasis patient serum and controls. Immunoblotting results indicate that reactivity to rSap2 was specific to candidiasis patient sera with no cross reactivity observed in healthy controls. Increased levels of anti-Sap2-specific Ig, IgG and IgM antibodies were observed in candidiasis patients compared to controls and was similar in sensitivity obtained when whole Candida was used as coating antigen. In summary, the rSap2 protein from C. parapsilosis has the potential to be used in the diagnosis of systemic candidiasis, providing a rapid, convenient, accurate and cost-effective strategy.
Abf1 Is an Essential Protein That Participates in Cell Cycle Progression and Subtelomeric Silencing in Candida glabrata
Authors: Hernández-Hernández Grecia , Vera-Salazar Laura A. , Castanedo Leonardo , López-Fuentes Eunice , Gutiérrez-Escobedo Guadalupe , De Las Peñas Alejandro , Castaño Irene
Published: December 2021

Accurate DNA replication and segregation is key to reproduction and cell viability in all organisms. Autonomously replicating sequence-binding factor 1 (Abf1) is a multifunctional protein that has essential roles in replication, transcription, and regional silencing in the model yeast Saccharomyces cerevisiae. In the opportunistic pathogenic fungus Candida glabrata, which is closely related to S. cerevisiae, these processes are important for survival within the host, for example, the regulation of transcription of virulence-related genes like those involved in adherence. Here, we describe that CgABF1 is an essential gene required for cell viability and silencing near the telomeres, where many adhesin-encoding genes reside. CgAbf1 mediated subtelomeric silencing depends on the 43 C-terminal amino acids. We also found that abnormal expression, depletion, or overexpression of Abf1, results in defects in nuclear morphology, nuclear segregation, and transit through the cell cycle. In the absence of ABF1, cells are arrested in G2 but start cycling again after 9 h, coinciding with the loss of cell viability and the appearance of cells with higher DNA content. Overexpression of CgABF1 causes defects in nuclear segregation and cell cycle progression. We suggest that these effects could be due to the deregulation of DNA replication.
Marine Macroalgae, a Source of Natural Inhibitors of Fungal Phytopathogens
Authors: Vicente Tânia F. L. , Lemos Marco F. L. , Félix Rafael , Valentão Patrícia , Félix Carina
Published: December 2021

Fungal phytopathogens are a growing problem all over the world; their propagation causes significant crop losses, affecting the quality of fruits and vegetables, diminishing the availability of food, leading to the loss of billions of euros every year. To control fungal diseases, the use of synthetic chemical fungicides is widely applied; these substances are, however, environmentally damaging. Marine algae, one of the richest marine sources of compounds possessing a wide range of bioactivities, present an eco-friendly alternative in the search for diverse compounds with industrial applications. The synthesis of such bioactive compounds has been recognized as part of microalgal responsiveness to stress conditions, resulting in the production of polyphenols, polysaccharides, lipophilic compounds, and terpenoids, including halogenated compounds, already described as antimicrobial agents. Furthermore, many studies, in vitro or in planta, have demonstrated the inhibitory activity of these compounds with respect to fungal phytopathogens. This review aims to gather the maximum of information addressing macroalgae extracts with potential inhibition against fungal phytopathogens, including the best inhibitory results, while presenting some already reported mechanisms of action.
Genome-Wide Identification and Functional Characterization of GATA Transcription Factor Gene Family in Alternaria alternata
Authors: Chen Yanan , Cao Yingzi , Gai Yunpeng , Ma Haijie , Zhu Zengrong , Chung Kuang-Ren , Li Hongye
Published: December 2021

In the present study, we identified six GATA transcription factors (AaAreA, AaAreB, AaLreA, AaLreB, AaNsdD, and AaSreA) and characterized their functions in response to environmental stress and virulence in the tangerine pathotype of Alternaria alternata. The targeted gene knockout of each of the GATA-coding genes decreased the growth to varying degrees. The mutation of AaAreA, AaAreB, AaLreB, or AaNsdD decreased the conidiation. All the GATA transcription factors were found to be required for tolerance to cumyl hydroperoxide and tert-butyl-hydroperoxide (oxidants) and Congo red (a cell-wall-destructing agent). Pathogenicity assays assessed on detached citrus leaves revealed that mutations of AaAreA, AaLreA, AaLreB, or AaNsdD significantly decreased the fungal virulence. A comparative transcriptome analysis between the ∆AreA mutant and the wild-type strain revealed that the inactivation of AaAreA led to alterations in the expression of genes involved in a number of biological processes, including oxidoreductase activity, amino acid metabolism, and secondary metabolite biogenesis. Taken together, our findings revealed that GATA-coding genes play diverse roles in response to environmental stress and are important regulators involved in fungal development, conidiation, ROS detoxification, as well as pathogenesis. This study, for the first time, systemically underlines the critical role of GATA transcription factors in response to environmental stress and virulence in A. alternata.
Colonzation of Tobacco Plants by Fungal Entomopathogens and the Effect on Consumption over Diabrotica speciosa (Coleoptera: Chrysomelidae)
Authors: Vianna Florencia , Pelizza Sebastian , Russo Leticia , Ferreri Natalia , Scorsetti Ana Clara
Published: December 2021

Entomopathogenic fungi are naturally occurring microorganisms that attack insect pests, making them exceptional allies when developing biocontrol strategies. A particular aspect of the ecology of these fungi is that they interact not only with insects but also with plants, being able to colonize them endophytically without causing symptoms of disease. The objectives of this study were to determine the endophytic capacity of different species of entomopathogenic fungi in tobacco plants by means of foliar spraying, to evaluate the persistence in planta of the entomopathogens and the effect of endophytes on consumption by coleopteran pests. A total of 24 strains were analyzed to test endophytic capacity at 7, 14, 21 and 28 days post inoculation. A significant effect of the strains was found. On days 7, 14 and 21, the strain that showed the highest colonization percentages was B. bassiana LPSc 1215, and at day 28 B. bassiana strains LPSc 1215 and LPSc 1212 exhibited the best endophytic capacity, maintaining elevated colonization percentages. Choice test results indicated that D. speciosa fed indiscriminately on colonized and non-colonized leaves. The results indicate that B. bassiana LPSc 1215 constitutes a strain that would merit further investigation for the purpose of pest management in tobacco cultivation.
Incidence, Speciation, and Morpho-Genetic Diversity of Penicillium spp. Causing Blue Mold of Stored Pome Fruits in Serbia
Authors: Žebeljan Aleksandra , Duduk Nataša , Vučković Nina , Jurick Wayne M. , Vico Ivana
Published: December 2021

Blue mold, caused by Penicillium spp., is one of the most economically important postharvest diseases of pome fruits, globally. Pome fruits, in particular apple, is the most widely grown pome fruit in Serbia, and the distribution of Penicillium spp. responsible for postharvest decay is unknown. A two-year survey was conducted in 2014 and 2015, where four pome fruits (apple, pear, quince, and medlar) with blue mold symptoms were collected from 20 storage locations throughout Serbia. Detailed morphological characterization, analysis of virulence in three apple cultivars, and multilocus phylogeny revealed three main Penicillium spp. in order of abundance: P. expansum, P. crustosum, and P. solitum. Interestingly, P. expansum split into two distinct clades with strong statistical support that coincided with several morphological observations. Findings from this study are significant and showed previously undocumented diversity in blue mold fungi responsible for postharvest decay including the first finding of P. crustosum, and P. solitum as postharvest pathogens of quince and P. crustosum of medlar fruit in the world, and P. expansum of quince in Serbia. Data from this study provide timely information regarding phenotypic, morphological and genotypic plasticity in P. expansum that will impact the design of species-specific detection tools and guide the development of blue mold management strategies.
Biotransformation of Chromium (VI) via a Reductant Activity from the Fungal Strain Purpureocillium lilacinum
Authors: Cárdenas González Juan Fernando , Acosta Rodríguez Ismael , Terán Figueroa Yolanda , Lappe Oliveras Patricia , Martínez Flores Rebeca , Rodríguez Pérez Adriana Sarai
Published: December 2021

Industrial effluents from chromium-based products lead to chromium pollution in the environment. Several technologies have been employed for the removal of chromium (Cr) from the environment, including adsorption, ion-exchange, bioremediation, etc. In this study, we isolated a Cr (VI)-resistant fungus, Purpureocillium lilacinum, from contaminated soil, which could reduce chromium. We also characterized a reductant activity of dichromate found in the cellular fraction of the fungus: optimal pH and temperature, effect of enzymatic inhibitors and enhancers, metal ions, use of electron donors, and initial Cr (VI) and protein concentration. This study also shows possible mechanisms that could be involved in the elimination of this metal. We observed an increase in the reduction of Cr (VI) activity in the presence of NADH followed by that of formate and acetate, as electron donor. This reduction was highly inhibited by EDTA followed by NaN3 and KCN, and this activity showed the highest activity at an optimal pH of 7.0 at 37 °C with a protein concentration of 3.62 µg/mL.
Effects of Laccaria bicolor on Gene Expression of Populus trichocarpa Root under Poplar Canker Stress
Authors: Dong Fengxin , Wang Yihan , Tang Ming
Published: December 2021

Poplars can be harmed by poplar canker. Inoculation with mycorrhizal fungi can improve the resistance of poplars to canker, but the molecular mechanism is still unclear. In this study, an aseptic inoculation system of L. bicolor–P. trichocarpa–B. dothidea was constructed, and transcriptome analysis was performed to investigate regulation by L. bicolor of the expression of genes in the roots of P. trichocarpa during the onset of B. dothidea infection, and a total of 3022 differentially expressed genes (DEGs) were identified. Weighted correlation network analysis (WGCNA) was performed on these DEGs, and 661 genes’ expressions were considered to be affected by inoculation with L. bicolor and B. dothidea. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that these 661 DEGs were involved in multiple pathways such as signal transduction, reactive oxygen metabolism, and plant-pathogen interaction. Inoculation with L. bicolor changed the gene expression pattern of the roots, evidencing its involvement in the disease resistance response of P. trichocarpa. This research reveals the mechanism of L. bicolor in inducing resistance to canker of P. trichocarpa at the molecular level and provides a theoretical basis for the practical application of mycorrhizal fungi to improve plant disease resistance.
Selection by UV Mutagenesis and Physiological Characterization of Mutant Strains of the Yeast Saprochaete suaveolens (Former Geotrichum fragrans) with Higher Capacity to Produce Flavor Compounds
Authors: Tan Melissa , Caro Yanis , Sing Alain Shum Cheong , Reiss Héloïse , Francois Jean-Marie , Petit Thomas
Published: December 2021

Yeast volatile organic compounds (VOCs), i.e. low molecular weight organic acids, alcohols and esters, are considered as potential and sustainable sources of natural aromas that can replace commonly used artificial flavors in food and other industrial sectors. Although research generally focuses on the yeast Saccharomyces cerevisiae, other so-called unconventional yeasts (NCY) are beginning to attract the attention of researchers, particularly for their ability to produce alternative panels of VOCs. With this respect, a Saprochaete suaveolens strain isolated from dragon fruit in Reunion Island was shown to produce α-unsaturated esters from branched-chain amino acids (BCAAs) such as isobutyl, isoamyl or ethyl tiglate, which are rarely found in other yeasts strains. Given that β-oxidation allows the growth of S. suaveolens on BCAAs as sole carbon source, we developped a method based on UV mutagenesis to generate mutants that can no longer grow on BCAAs, while redirecting the carbon flow towards esterification of α-unsaturated esters. Among the 15,000 clones generated through UV irradiation, we identified nine clones unable to grow on BCAAs with one of them able to produce eight times more VOCs as compared to the wild-type strain. This higher production of α-unsaturated esters in this mutant strain coincided with an almost complete loss of enoyl-CoA hydratase activity of the β-oxidation pathways and with a twofold increase of acyl-CoA hydrolase with not significant changes in the enzymes of the Ehrlich pathway. Moreover, from our knowledge, it constituted the first example of VOCs enhancement in a microbial strain by UV mutagenesis.
How Are Women Politicians Treated in the Press? The Case of Spain, France and the United Kingdom
Authors: Fernández-Rovira Cristina , Giraldo-Luque Santiago
Published: December 2021

Women politicians have been discriminated against or negatively valued under stereotypes in media coverage and have been given a secondary role compared to male politicians. The article proposes an analysis of the treatment given by digital media to women political leaders. They are from different parties in three countries and the aim is to identify the polarity (positive, neutral or negative) of the information published about them in the media. The text focuses on the cases of Anne Hidalgo and Marine Le Pen, from France, Nicola Sturgeon and Theresa May, from the United Kingdom and Ada Colau and Inés Arrimadas, from Spain. The study develops a computerised sentiment analysis of the information published in two leading digital newspapers in each country, during the month of November 2019. The research, with the analysis of 1100 journalistic pieces, shows that the polarity or valence of the women analysed is predominantly neutral and positive and that the journalistic genres do not determine the media representation of the women studied. On the contrary, the country of study does have a predominant incidence on the way in which women politicians are represented, while the relationship of affinity or antipathy of the Spanish media with the women politicians studied is significant.
Evaluation of the Financial Performance of the Municipalities in Slovakia in the Context of Multidimensional Statistics
Authors: Lukáč Jozef , Teplická Katarína , Čulková Katarína , Hrehová Daniela
Published: December 2021

In some studies, only financial aspects are emphasized, but we also see cases of assessing the financial health of municipalities through socio-economic indicators. Public organizations worldwide have had to increase their financial performance by adopting management practices. Nonetheless, financial performance might be mostly predicted by contingencies that are not within direct managerial control. The purpose of this paper is to identify clusters of municipalities on the basis of agglomerate cluster analysis, the results of which will point to the financial situation of the municipalities in the selected region. The main aim of this contribution is to identify the location of the municipalities of the chosen self-governing region of Slovakia using the clustering method by selected financial indicators. Individual clusters have similar properties and they differ from the characteristics of businesses in other clusters. The results show that organizational and environmental contingencies affect financial performance, but a significant amount of variation in financial performance is unexplained—indicating that management creates better financial health in the municipality and creates a clearer budget for the management, employees, and residents of the municipality.