bluuebunny/crossref_metadata_2025 huggingface dataset:
Care for Staff: A Novel Technical Assistance Approach to Promote Occupational Mental Health Among Healthcare Workers in Lower and Middle Income Country Settings
Authors: Headrick Emily G. , McCarten-Gibbs Maggie , Coley Rachel , Avila Graciela , Mina Jerome , Villa Rea Celine , Fernando L. Harini
Published: April 2024

Background:

Healthcare workers (HCWs) in lower- and middle-income countries (LMICs) face unique, intersectional threats to their mental health at work. Despite the existence of recommendations for multi-tiered interventions to promote and protect occupational mental health for HCWs, there remain significant challenges to implementation worldwide.

Methods:

FHI 360, a global development organization, developed a novel technical assistance framework to accompany partners, including government and healthcare leaders to design, implement, improve, or evaluate any mental health and psychosocial support intervention. The EpiC Project, implemented by FHI 360, has utilized this framework in four countries (Vietnam, Philippines, Paraguay and Sri Lanka) specifically to guide the development of locally adapted occupational mental health interventions for HCWs.

Findings:

Each country applied this framework in various project cycle phases and in their unique local contexts; all countries reported positive developments in the advancement of their chosen interventions.

Conclusions/Application to Practice:

With the application of an adaptable, evidence-based technical assistance framework to guide collaborative consultation for project design, implementation, improvement, and/or evaluation, locally led teams pivoted from a solely “mental health” approach to more comprehensive, evidence-based interventions that framed mental health for HCWs as an occupational health priority. This allowed for teams advising interventions in LMICs to consider unique workplace, structural and policy-level factors rather than focusing solely on individual mental health strategies.
(Why) Do Transparent Hearing Devices Impair Speech Perception in Collocated Noise?
Authors: Denk Florian , Wiederschein Luca , Kemper Markus , Husstedt Hendrik
Published: April 2024

Hearing aids and other hearing devices should provide the user with a benefit, for example, compensate for effects of a hearing loss or cancel undesired sounds. However, wearing hearing devices can also have negative effects on perception, previously demonstrated mostly for spatial hearing, sound quality and the perception of the own voice. When hearing devices are set to transparency, that is, provide no gain and resemble open-ear listening as well as possible, these side effects can be studied in isolation. In the present work, we conducted a series of experiments that are concerned with the effect of transparent hearing devices on speech perception in a collocated speech-in-noise task. In such a situation, listening through a hearing device is not expected to have any negative effect, since both speech and noise undergo identical processing, such that the signal-to-noise ratio at ear is not altered and spatial effects are irrelevant. However, we found a consistent hearing device disadvantage for speech intelligibility and similar trends for rated listening effort. Several hypotheses for the possible origin for this disadvantage were tested by including several different devices, gain settings and stimulus levels. While effects of self-noise and nonlinear distortions were ruled out, the exact reason for a hearing device disadvantage on speech perception is still unclear. However, a significant relation to auditory model predictions demonstrate that the speech intelligibility disadvantage is related to sound quality, and is most probably caused by insufficient equalization, artifacts of frequency-dependent signal processing and processing delays.
Settler ecologies and more-than-One Health: From malaria to avian flu in the Hula Valley, Palestine-Israel
Authors: Braverman Irus
Published: April 2024

The story of the Hula Valley in the Galilee region of Palestine-Israel serves as the focus of this article, which draws on the concepts “more-than-One Health” and “settler ecologies” to highlight the harmful ecological implications of settler colonial projects in this region and elsewhere. Specifically, I tell the story of the Zionist drying of the Hula wetlands in the 1950s for the purpose of fighting off malaria and advancing agriculture in the region—and then of Israel's reflooding and rehabilitation of parts of the Hula in the 1990s in support of the massive annual bird migration. In winter 2021, more than eight thousand cranes succumbed to an avian influenza (H5N1) outbreak in the Hula Valley and over one million chickens in the area's coops had to be culled. Drawing on ethnographic fieldwork conducted mainly in summer 2022, this article discusses the historical and socioecological conditions that have arguably enabled and exacerbated the avian outbreak, advocating for a more-than-One Health approach that is founded on acknowledging the settler colonial legacies of this place.
Sealing of Iatrogenic Distal Coronary Artery Perforation by Liquid Embolization Agent—A Case Report
Authors: Khan Saherish , Ram Gudipati Ananta , Sudhakar B. G. K. , Jain Rajendra Kumar
Published: April 2024

Percutaneous coronary intervention (PCI) is one of the most commonly performed cardiovascular interventions to treat significant coronary artery stenosis. Although a safe procedure, it carries the risk of complications like coronary artery perforation (CAP), bleeding, stroke, and acute kidney injury rarely resulting in death. The incidence of CAP is 0.84% of all PCI procedures and the incidence is increasing as cardiologists are accepting more and more challenging cases. CAP is caused by a guidewire (GW) or a vessel tear from balloon angioplasty, stenting, or rotational atherectomy. Though the majority of CAPs do not result in cardiac tamponade, some do lead to tamponade and death. The majority of distal wire perforations respond to prolonged balloon inflation, coils, glue, or fat embolization. Herein, we report a case of distal CAP in a 63-year-old woman occurring following left circumflex artery PCI caused by the exiting of coronary GW. The CAP was successfully sealed by the liquid embolization technique using ONYX. First described in 1990, Onyx is an elastic polymer composed of ethylene vinyl alcohol copolymer dissolved in dimethyl sulfoxide and mixed with micronized tantalum powder (Onyx). The Onyx is predominantly used by interventional radiologists while performing neurovascular and peripheral vascular interventions. There were only a few case reports of plugging of CAPs using Onyx. Our case highlights the safety and feasibility of closing distal CAPs using Onyx.
The experiences of Black African parents on partnership: Working with education professionals in the English special education needs and disability system
Authors: Nnalue Chike , Mahmud Arif
Published: April 2024

Reforms to special education policy in England have emphasised the importance of parents being involved in decision-making about their child’s education and greater control over their child’s support. This study’s research questions investigated four Black African parents of children with special educational needs and disability (SEND) living in England and their experiences of working in partnership with education professionals to support their children, as well as exploring the barriers to effective partnership between Black African parents and education professionals. A critical approach was taken to understand how issues of power such as race and socio-economic status, combined with the parents own African culture, affected their interactions with education professionals who hold an entirely different contextual disposition. The parents took part in semi-structured interviews which were thematically analysed. Three overarching themes were identified: Communication (which focused on issues such as language, articulation and communication styles ); cultural barriers (which encompassed the cultural differences between the parents and the education professionals) ; and the parent-professional relationship (which was key for the parents to navigate the SEND system). Recommendations for practice such as professionals building more trust with parents, engaging more with cultural competency training and evaluating the accessibility of their current services are discussed.
Experiences of social isolation and loneliness in middle-aged and older autistic adults
Authors: Stewart Gavin R. , Luedecke Emily , Mandy William , Charlton Rebecca A. , Happé Francesca
Published: April 2024

Background

Young autistic people often experience social isolation and loneliness. Despite social isolation and loneliness increasing in later life in non-autistic populations, no studies have examined whether middle-aged and older autistic adults are at an increased likelihood of these experiences.

Methods

428 adults (autistic n = 265) aged 40–93 years (mean = 60.5 years) completed questionnaires related to social connectedness/isolation, loneliness, and mental health. Group differences and associations were examined.

Results

The autistic group was less socially connected and lonelier than the non-autistic group. While there were few gender differences in social connectedness, women were lonelier than men in both groups, including when controlling for age and mental health. Social connectedness decreased with age in both groups, loneliness only increased with age in the autistic group. For both social connectedness and loneliness, age associations were significantly stronger in men than women.

Conclusions

Autistic adults may be particularly susceptible to social isolation and loneliness in midlife and older age. While autistic women were lonelier than autistic men, autistic men may be at greater risk of increasing social isolation and loneliness with older age. This study highlights the need for evidence-based interventions to address social isolation and to reduce loneliness for autistic people as they age.
Intraspecific demographic and trait responses to environmental change drivers are linked in two species of ciliate
Authors: de Bruin Tessa , De Laender Frederik , Jadoul Julie , Schtickzelle Nicolas
Published: April 2024

Background

Over the past decade, theory and observations have suggested intraspecific variation, trait-based differences within species, as a buffer against biodiversity loss from multiple environmental changes. This buffering effect can only occur when different populations of the same species respond differently to environmental change. More specifically, variation of demographic responses fosters buffering of demography, while variation of trait responses fosters buffering of functioning. Understanding how both responses are related is important for predicting biodiversity loss and its consequences. In this study, we aimed to empirically assess whether population-level trait responses to multiple environmental change drivers are related to the demographic response to these drivers. To this end, we measured demographic and trait responses in microcosm experiments with two species of ciliated protists. For three clonal strains of each species, we measured responses to two environmental change drivers (climate change and pollution) and their combination. We also examined if relationships between demographic and trait responses existed across treatments and strains.

Results

We found different demographic responses across strains of the same species but hardly any interactive effects between the two environmental change drivers. Also, trait responses (summarized in a survival strategy index) varied among strains within a species, again with no driver interactions. Demographic and trait responses were related across all strains of both species tested in this study: Increasing intrinsic growth and self-limitation were associated with a shift in survival strategy from sit-and-wait towards flee.

Conclusions

Our results support the existence of a link between a population’s demographic and trait responses to environmental change drivers in two species of ciliate. Future work could dive deeper into the specifics of phenotypical trait values, and changes therein, related to specific life strategies in different species of ciliate and other zooplankton grazers.
Genome-wide identification and stress response analysis of BcaCPK gene family in amphidiploid Brassica carinata
Authors: Zuo Dan , Lei Shaolin , Qian Fang , Gu Lei , Wang Hongcheng , Du Xuye , Zeng Tuo , Zhu Bin
Published: April 2024

Background

Calcium-dependent protein kinases (CPKs) are crucial for recognizing and transmitting Ca2+ signals in plant cells, playing a vital role in growth, development, and stress response. This study aimed to identify and detect the potential roles of the CPK gene family in the amphidiploid Brassica carinata (BBCC, 2n = 34) using bioinformatics methods.

Results

Based on the published genomic information of B. carinata, a total of 123 CPK genes were identified, comprising 70 CPK genes on the B subgenome and 53 on the C subgenome. To further investigate the homologous evolutionary relationship between B. carinata and other plants, the phylogenetic tree was constructed using CPKs in B. carinata and Arabidopsis thaliana. The phylogenetic analysis classified 123 family members into four subfamilies, where gene members within the same subfamily exhibited similar conserved motifs. Each BcaCPK member possesses a core protein kinase domain and four EF-hand domains. Most of the BcaCPK genes contain 5 to 8 introns, and these 123 BcaCPK genes are unevenly distributed across 17 chromosomes. Among these BcaCPK genes, 120 replicated gene pairs were found, whereas only 8 genes were tandem duplication, suggesting that dispersed duplication mainly drove the family amplification. The results of the Ka/Ks analysis indicated that the CPK gene family of B. carinata was primarily underwent purification selection in evolutionary selection. The promoter region of most BcaCPK genes contained various stress-related cis-acting elements. qRT-PCR analysis of 12 selected CPK genes conducted under cadmium and salt stress at various points revealed distinct expression patterns among different family members in response to different stresses. Specifically, the expression levels of BcaCPK2.B01a, BcaCPK16.B02b, and BcaCPK26.B02 were down-regulated under both stresses, whereas the expression levels of other members were significantly up-regulated under at least one stress.

Conclusion

This study systematically identified the BcaCPK gene family in B. carinata, which contributes to a better understanding the CPK genes in this species. The findings also serve as a reference for analyzing stress responses, particularly in relation to cadmium and salt stress in B. carinata.
Distinct experiences and care needs of advanced cancer patients with good ECOG performance status: a qualitative phenomenological study
Authors: Chen Ping , Ding Mingfu , Li Changlin , Long Yujuan , Pan Deng , Ma Li , Liu Taiguo , Yi Cheng
Published: April 2024

Background

Advanced cancer patients with good Eastern Cooperative Oncology Group (ECOG) performance status (score 0–1) are underrepresented in current qualitative reports compared with their dying counterparts.

Aim

To explore the experiences and care needs of advanced cancer patients with good ECOG.

Design

A qualitative phenomenological approach using semi-structured interview was employed. Data was analyzed using the Colaizzi’s method.

Setting/Participants

Purposive sample of terminal solid cancer patients on palliative care aged 18–70 years with a 0–1 ECOG score were recruited from a tertiary general hospital.

Results

Sixteen participants were interviewed. Seven themes were generated from the transcripts, including experiencing no or mild symptoms; independence in self-care, decision-making, and financial capacity; prioritization of cancer growth suppression over symptom management; financial concerns; hope for prognosis and life; reluctance to discuss death and after-death arrangements; and use of complementary and alternative medicine (CAM) and religious coping.

Conclusions

Advanced cancer patients with good ECOG have distinct experiences and care needs from their dying counterparts. They tend to experience no or mild symptoms, demonstrate a strong sense of independence, and prioritize cancer suppression over symptom management. Financial concerns were common and impact their care-related decision-making. Though being hopeful for their prognosis and life, many are reluctant to discuss death and after-death arrangements. Many Chinese patients use herbal medicine as a CAM modality but need improved awareness of and accessibility to treatment options. Healthcare professionals and policy-makers should recognize their unique experiences and needs when tailoring care strategies and policies.
Sustaining the nursing workforce - exploring enabling and motivating factors for the retention of returning nurses: a qualitative descriptive design
Authors: Yamamoto Kumiko , Nasu Katsumi , Nakayoshi Yoko , Takase Miyuki
Published: April 2024

Background

The nursing shortage represents a persistent and urgent challenge within the healthcare industry. One of the most cost-effective and time-efficient solutions to address this issue is the recruitment of inactive nurses to rejoin the nursing workforce, while simultaneously ensuring the long-term sustainability of their careers following their return to work. The aim of this study is to explore the factors that facilitate the retention of nurses who have returned to work, from their perspective.

Methods

To achieve this aim, a qualitative descriptive design was employed. A total of 15 registered nurses who had not practiced nursing for a minimum of three years prior to their return to work, and had been working as nurses for at least three months following their return, were selected from seven healthcare institutions using convenience sampling. Face-to-face or online semi-structured interviews were conducted, and qualitative inductive analysis was employed to analyze the collected data.

Results

The analysis revealed five key themes, two of which were related to the enabling factors making it possible for the nurses to continue their work, while the remaining three pertained to the motivating factors driving the pursuit of professional careers. The two themes associated with enabling factors were identified as “Conditions and support that sustain work-life balance” and “A workplace that acknowledges my career, and encourages my growth as an experienced nurse”. The three themes related to motivating factors were entitled “Pride in reconnecting with and contributing to society,” “Cultivating confidence through incremental professional development and future envisioning,” and “Enrichment of my own and my family’s life”.

Conclusions

Returning nurses constitute a valuable asset for healthcare institutions. To effectively retain these nurses, it is crucial to implement multi-dimensional approaches that enable and motivate them to sustain and enrich their professional and personal lives while continuing their work in the nursing field.
Swim bladder-derived biomaterials: structures, compositions, properties, modifications, and biomedical applications
Authors: Lan Xiaorong , Luo Mingdong , Li Meiling , Mu Linpeng , Li Guangwen , Chen Gong , He Zhoukun , Xiao Jingang
Published: April 2024

Animal-derived biomaterials have been extensively employed in clinical practice owing to their compositional and structural similarities with those of human tissues and organs, exhibiting good mechanical properties and biocompatibility, and extensive sources. However, there is an associated risk of infection with pathogenic microorganisms after the implantation of tissues from pigs, cattle, and other mammals in humans. Therefore, researchers have begun to explore the development of non-mammalian regenerative biomaterials. Among these is the swim bladder, a fish-derived biomaterial that is rapidly used in various fields of biomedicine because of its high collagen, elastin, and polysaccharide content. However, relevant reviews on the biomedical applications of swim bladders as effective biomaterials are lacking. Therefore, based on our previous research and in-depth understanding of this field, this review describes the structures and compositions, properties, and modifications of the swim bladder, with their direct (including soft tissue repair, dural repair, cardiovascular repair, and edible and pharmaceutical fish maw) and indirect applications (including extracted collagen peptides with smaller molecular weights, and collagen or gelatin with higher molecular weights used for hydrogels, and biological adhesives or glues) in the field of biomedicine in recent years. This review provides insights into the use of swim bladders as source of biomaterial; hence, it can aid biomedicine scholars by providing directions for advancements in this field.
Synovium microenvironment-responsive injectable hydrogel inducing modulation of macrophages and elimination of synovial fibroblasts for enhanced treatment of rheumatoid arthritis
Authors: Wu Yiqun , Ge Yu , Wang Zhongshi , Zhu Ying , Tian Tianli , Wei Jun , Jin Yu , Zhao Yi , jia Qiang , Wu Jun , Ge Liang
Published: April 2024

Rheumatoid arthritis (RA) is a progressive autoimmune disease accompanied by joint swelling, cartilage erosion and bone damage. Drug therapy for RA has been restricted due to poor therapeutic effect, recurrence and adverse effects. Macrophages and synovial fibroblasts both play important roles in the pathology of RA. Macrophages secrete large amount of pro-inflammatory cytokines, while synovial fibroblasts are tightly correlated with hypoxia synovium microenvironment, cytokine release, recruitment of pro-inflammatory cells, bone and cartilage erosion. Therefore, in this timely research, an injectable and pH-sensitive peptide hydrogel loading methotrexate (MTX) and bismuthene nanosheet/polyethyleneimine (BiNS/PEI) has been developed to reduce the activity of macrophages and eliminate over-proliferated synovial fibroblasts simultaneously. MTX can reduce the cytokine secretion of macrophages/anti-apoptosis property of synovial fibroblasts and BiNS/PEI can eliminate synovial fibroblasts via photodynamic therapy (PDT) and photothermal therapy (PTT) routes. The hydrogel was injected into the acidic inflammatory synovium for precise targeting and served as a drug reservoir for pH responsive and sustained drug release, while improving the bioavailability and reducing the toxicity of MTX. Excellent therapeutic efficacy has been achieved in both in vivo and in vitro studies, and this unique drug delivery system provides a new and robust strategy to eliminate synovial fibroblasts and modulate immune system for RA treatment in clinical.

Graphic Abstract
Effect of preoperative immunonutrition on postoperative short-term clinical outcomes in patients with gastric cancer cachexia: a prospective randomized controlled trial
Authors: Yu Junjian , Yuan Antai , Liu Qi , Wang Wei , Sun Yuqi , Li Zequn , Meng Cheng , Zhou Yanbing , Cao Shougen
Published: April 2024

Background

Although current guidelines(ESPEN guideline: Clinical nutrition in surgery and other guidelines) recommend preoperative immunonutrition for cachectic gastric cancer patients, the strength of the recommendation is weak, and the level of evidence is low. The benefits of preoperative immunonutrition still remain controversial.

Patients and methods

112 patients with gastric cancer cachexia were enrolled in the study and randomly assigned in a 1:1 ratio to receive either preoperative enteral immunonutrition support (IN, n = 56) or standard enteral nutrition support (SEN, n = 56). The primary endpoint was the incidence of infectious complications, and the secondary endpoints included the nutritional indicators, inflammatory markers, immune parameters, postoperative recovery and complications and gastrointestinal intolerance reactions.

Results

The incidence of postoperative infectious complications(P = 0.040) and overall complications (P = 0.049)was significantly lower in the IN group compared to the SEN group. In terms of laboratory inflammatory indexes, patients in the IN group demonstrated significantly lower levels of white blood cells (WBC), C-reactive protein (CRP), and interleukin-6 (IL-6), as well as higher levels of lymphocytes (LYMPH) and immunoglobulin A (IgA), compared to patients in the SEN group, with statistically significant differences. In terms of clinical outcomes, the IN group had a shorter duration of antibiotic use (P = 0.048), shorter hospital stay (P = 0.018), and lower total hospital costs (P = 0.034) compared to the SEN group. The IN group also experienced significantly less weight loss after surgery (P = 0.043).

Conclusion

Preoperative administration of immunonutrition formula has a positive impact on the incidence of infectious complications in patients with gastric cancer cachexia after surgery. It improves patients’ inflammatory and immune status, shortens hospital stays, and reduces healthcare costs. Preoperative use of immunonutrition may contribute to the improvement of prognosis in this high-risk population.
Prevalence of diverse colorectal polyps and risk factors for colorectal carcinoma in situ and neoplastic polyps
Authors: Li Xiaojuan , Hu Mengting , Wang Zhangjun , Liu Mei , Chen Ying
Published: April 2024

Background

Most colorectal cancers originate from precancerous polyps. This study aimed to determine the prevalence of colorectal polyps with diverse pathological morphologies and to explore the risk factors for colorectal carcinoma in situ (CCS) and neoplastic polyps.

Methods

Inpatients admitted from January 2018 to May 2023 were screened through the hospital information system. Polyps were classified according to pathological morphology. The prevalence of polyps was described by frequency and 95% confidence interval. Univariate and multivariate logistic regression analyses were used to explore the risk factors for CCS and neoplastic polyps.

Results

In total, 2329 individuals with 3550 polyps were recruited. Among all patients, 76.99% had neoplastic polyps and 44.31% had advanced adenomas. Tubular adenoma had the highest prevalence at 60.15%, and the prevalence of CCS was 3.86%. Patients with a colorectal polyp diameter ≥ 1.0 cm or number ≥ 3 were 8.07 times or 1.98 times more likely to develop CCS than were those with a diameter < 1.0 cm or number < 3, respectively (OR 8.07, 95%CI 4.48–14.55, p < 0.0001; and OR 1.98, 95%CI 1.27–3.09, p = 0.002). The risk of CCS with schistosome egg deposition was also significantly increased (OR 2.70, 95%CI 1.05–6.98). The higher the levels of carbohydrate antigen (CA) 724 (OR 1.01, 95%CI 1.00–1.02) and CA211 (OR 1.16, 95%CI 1.03–1.32) in patients with colorectal polyps were, the greater the risk of CCS. When colorectal neoplastic polyps were analyzed, we discovered that for each 1-year increase in age, the risk of neoplastic polyps increased by 3% (OR 1.03, 95%CI 1.02–1.04), p < 0.0001. Patients with a polyp diameter ≥ 1.0 cm had a 2.11-fold greater risk of neoplastic polyps compared to diameter < 1.0 cm patients (OR 3.11, 95%CI 2.48–3.92), p < 0.0001. In addition, multiple polyps and CA199 levels are risk factors for neoplastic polyps.

Conclusion

More than 3/4 of colorectal polyp patients have neoplastic polyps. Patients are more inclined to develop CCS and neoplastic polyps if they have large polyps (> 1.0 cm) or multifocal polyps. The levels of the tumor markers CA724 and CA211 show some potential usefulness for predicting CCS and may be exploited for early identification of high-risk populations.
Incorporation, adaptation and rejection of obstetric practices during the implementation of the “Adequate Childbirth Program” in Brazilian private hospitals: a qualitative study
Authors: de Oliveira Débora Cecília Chaves , Gomes Maysa Luduvice , Rodrigues Andreza , Soares Thamires , de Azevedo Nicida Lucia Regina , Torres Jacqueline Alves , Engstrom Elyne Montenegro
Published: April 2024

Background

The “Adequate Childbirth Program” (PPA) is a quality improvement project that aims to reduce the high rates of unnecessary cesarean section in Brazilian private hospitals. This study aimed to analyze labor and childbirth care practices after the first phase of PPA implementation.

Method

This study uses a qualitative approach. Eight hospitals were selected. At each hospital, during the period of 5 (five) days, from July to October 2017, the research team conducted face to face interviews with doctors (n = 21) and nurses (n = 28), using semi-structured scripts. For the selection of professionals, the Snowball technique was used. The interviews were transcribed, and the data submitted to Thematic Content Analysis, using the MaxQda software.

Results

The three analytical dimensions of the process of change in the care model: (1) Incorporation of care practices: understood as the practices that have been included since PPA implementation; (2) Adaptation of care practices: understood as practices carried out prior to PPA implementation, but which underwent modifications with the implementation of the project; (3) Rejection of care practices: understood as those practices that were abandoned or questioned whether or not they should be carried out by hospital professionals.

Conclusions

After the PPA, changes were made in hospitals and in the way, women were treated. Birth planning, prenatal hospital visits led by experts (for expecting mothers and their families), diet during labor, pharmacological analgesia for vaginal delivery, skin-to-skin contact, and breastfeeding in the first hour of life are all included. To better monitor labor and vaginal birth and to reduce CS without a clinical justification, hospitals adjusted their present practices. Finally, the professionals rejected the Kristeller maneuver since research has demonstrated that using it’s harmful.
Decoding dynamic miRNA:ceRNA interactions unveils therapeutic insights and targets across predominant cancer landscapes
Authors: Ari Yuka Selcen , Yilmaz Alper
Published: April 2024

Competing endogenous RNAs play key roles in cellular molecular mechanisms through cross-talk in post-transcriptional interactions. Studies on ceRNA cross-talk, which is particularly dependent on the abundance of free transcripts, generally involve large- and small-scale studies involving the integration of transcriptomic data from tissues and correlation analyses. This abundance-dependent nature of ceRNA interactions suggests that tissue- and condition-specific ceRNA dynamics may fluctuate. However, there are no comprehensive studies investigating the ceRNA interactions in normal tissue, ceRNAs that are lost and/or appear in cancerous tissues or their interactions. In this study, we comprehensively analyzed the tumor-specific ceRNA fluctuations observed in the three highest-incidence cancers, LUAD, PRAD, and BRCA, compared to healthy lung, prostate, and breast tissues, respectively. Our observations pertaining to tumor-specific competing endogenous RNA (ceRNA) interactions revealed that, in the cases of lung adenocarcinoma (LUAD), prostate adenocarcinoma (PRAD), and breast invasive carcinoma (BRCA), 3,204, 1,233, and 406 ceRNAs, respectively, engage in post-transcriptional intercommunication within tumor tissues, in contrast to their absence in corresponding healthy samples. We also found that 90 ceRNAs are shared by the three cancer types and that these ceRNAs participate in ceRNA interactions in tumor tissues compared to those in normal tissues. Among the 90 ceRNAs that directly interact with miRNAs, we uncovered a core network of 165 miRNAs and 63 ceRNAs that should be considered in RNA-targeted and RNA-mediated approaches in future studies and could be used in these three aggressive cancer types. More specifically, in this core interaction network, ceRNAs such as GALNT7, KLF9, and DAB2 and miRNAs like miR-106a/b-5p, miR-20a-5p, and miR-519d-3p may have potential as common targets in the three critical cancers. In contrast to conventional methods that construct ceRNA networks using differentially expressed genes compared to normal tissues, our proposed approach identifies ceRNA players by considering their context within the ceRNA:miRNA interactions. Our results have the potential to reveal distinct and common ceRNA interactions in cancer types and to pinpoint critical RNAs, thereby paving the way for RNA-based strategies in the battle against cancer.
Mitochondrial complex I density is associated with IQ and cognition in cognitively healthy adults: an in vivo [18F]BCPP-EF PET study
Authors: Shatalina Ekaterina , Whitehurst Thomas S. , Onwordi Ellis Chika , Gilbert Barnabas J. , Rizzo Gaia , Whittington Alex , Mansur Ayla , Tsukada Hideo , Marques Tiago Reis , Natesan Sridhar , Rabiner Eugenii A. , Wall Matthew B. , Howes Oliver D.
Published: April 2024

Background

Mitochondrial function plays a key role in regulating neurotransmission and may contribute to general intelligence. Mitochondrial complex I (MC-I) is the largest enzyme of the respiratory chain. Recently, it has become possible to measure MC-I distribution in vivo, using a novel positron emission tomography tracer [18F]BCPP-EF, thus, we set out to investigate the association between MC-I distribution and measures of cognitive function in the living healthy brain.

Results

Analyses were performed in a voxel-wise manner and identified significant associations between [18F]BCPP-EF DVRCS−1 in the precentral gyrus and parietal lobes and WAIS-IV predicted IQ, WAIS-IV arithmetic and WAIS-IV symbol-digit substitution scores (voxel-wise Pearson’s correlation coefficients transformed to Z-scores, thresholded at Z = 2.3 family-wise cluster correction at p < 0.05, n = 16). Arithmetic scores were associated with middle frontal and post-central gyri tracer uptake, symbol-digit substitution scores were associated with precentral gyrus tracer uptake. RAVLT recognition scores were associated with [18F]BCPP-EF DVRCS−1 in the middle frontal gyrus, post-central gyrus, occipital and parietal regions (n = 20).

Conclusions

Taken together, our findings support the theory that mitochondrial function may contribute to general intelligence and indicate that interindividual differences in MC-I should be a key consideration for research into mitochondrial dysfunction in conditions with cognitive impairment.
Joint beamforming and power splitting design for MISO downlink communication with SWIPT: a comparison between cell-free massive MIMO and small-cell deployments
Authors: Liu Jain-Shing , Lin Chun-Hung Richard , Chang Wan-Ling
Published: April 2024

Simultaneous wireless information and power transfer (SWIPT) has been advocated as a highly promising technology for enhancing the capabilities of 5G and 6G devices. However, the challenge of dealing with large propagation path loss poses a significant hurdle. To address this issue, massive multiple-input multiple-output (MIMO) is employed to enhance the efficiency of SWIPT in cellular-based networks with multiple small cells, and especially increase the energy for cell-edge users. In addition, by leveraging a large set of spatially distributed base stations to collaboratively serve SWIPT-enabled user equipment, the cell-free massive MIMO has the potential to provide even better performance than the conventional small-cell systems. In this work, we extend the investigation to include the application of SWIPT technology with alternating current (AC) logic in the cell-free networks and the small-cell networks and propose joint beamforming and power splitting optimization frameworks to maximize the system sum-rate, subject to the constraints on harvested energy, AC logic energy supply, and total transmit power. The optimization problem is shown to be non-convex, posing a significant challenge. To address this challenge, we resort to a two-stage decomposition approach. Specifically, we first introduce quadratic transform-based fractional programming (FP) algorithms to iteratively solve the non-convex optimization problems in the first stage, achieving near-optimal solutions with low time complexities. To further reduce the complexities, we also incorporate conventional schemes such as zero forcing, maximum ratio transmission, and signal-to-leakage-and-noise ratio for the design of beamforming vectors. Second, to determine the optimal power splitting ratio within the framework, we develop a one-dimensional (1-D) search algorithm to tackle the single variable optimization problem reduced in the second stage. These algorithms are then evaluated in the context of cell-free MIMO and small-cell networks with numerical experiments. The results show that the FP-based algorithms can consistently outperform those utilizing the conventional beamforming schemes, and the solutions of this work can achieve up to fivefold improvement in the system sum-rate than the small-cell counterpart while providing different but comparable performance trends in energy harvesting (EH).
Decline of a distinct coral reef holobiont community under ocean acidification
Authors: Williams Jake , Pettorelli Nathalie , Hartmann Aaron C. , Quinn Robert A. , Plaisance Laetitia , O’Mahoney Michael , Meyer Chris P. , Fabricius Katharina E. , Knowlton Nancy , Ransome Emma
Published: April 2024

Background

Microbes play vital roles across coral reefs both in the environment and inside and upon macrobes (holobionts), where they support critical functions such as nutrition and immune system modulation. These roles highlight the potential ecosystem-level importance of microbes, yet most knowledge of microbial functions on reefs is derived from a small set of holobionts such as corals and sponges. Declining seawater pH — an important global coral reef stressor — can cause ecosystem-level change on coral reefs, providing an opportunity to study the role of microbes at this scale. We use an in situ experimental approach to test the hypothesis that under such ocean acidification (OA), known shifts among macrobe trophic and functional groups may drive a general ecosystem-level response extending across macrobes and microbes, leading to reduced distinctness between the benthic holobiont community microbiome and the environmental microbiome.

Results

We test this hypothesis using genetic and chemical data from benthic coral reef community holobionts sampled across a pH gradient from CO2 seeps in Papua New Guinea. We find support for our hypothesis; under OA, the microbiome and metabolome of the benthic holobiont community become less compositionally distinct from the sediment microbiome and metabolome, suggesting that benthic macrobe communities are colonised by environmental microbes to a higher degree under OA conditions. We also find a simplification and homogenisation of the benthic photosynthetic community, and an increased abundance of fleshy macroalgae, consistent with previously observed reef microbialisation.

Conclusions

We demonstrate a novel structural shift in coral reefs involving macrobes and microbes: that the microbiome of the benthic holobiont community becomes less distinct from the sediment microbiome under OA. Our findings suggest that microbialisation and the disruption of macrobe trophic networks are interwoven general responses to environmental stress, pointing towards a universal, undesirable, and measurable form of ecosystem change.
Epidemiologic association and shared genetic architecture between cataract and hearing difficulties among middle-aged and older adults
Authors: Zhang Xiayin , Wang Shan , Liu Shunming , Du Zijing , Wu Guanrong , Liang Yingying , Huang Yu , Shang Xianwen , Hu Yijun , Zhu Zhuoting , Sun Wei , Zhang Xueli , Yu Honghua
Published: April 2024

Age-related cataract and hearing difficulties are major sensory disorders that often co-exist in the global-wide elderly and have a tangible influence on the quality of life. However, the epidemiologic association between cataract and hearing difficulties remains unexplored, while little is known about whether the two share their genetic etiology. We first investigated the clinical association between cataract and hearing difficulties using the UK Biobank covering 502,543 individuals. Both unmatched analysis (adjusted for confounders) and a matched analysis (one control matched for each patient with cataract according to confounding factors) were undertaken and confirmed that cataract was associated with hearing difficulties (OR, 2.12; 95% CI, 1.98–2.27; OR, 2.03; 95% CI, 1.86–2.23, respectively). Furthermore, we explored and quantified the shared genetic architecture of these two complex sensory disorders at the common variant level using the bivariate causal mixture model (MiXeR) and conditional/conjunctional false discovery rate method based on the largest available genome-wide association studies of cataract (N = 585,243) and hearing difficulties (N = 323,978). Despite detecting only a negligible genetic correlation, we observe polygenic overlap between cataract and hearing difficulties and identify 6 shared loci with mixed directions of effects. Follow-up analysis of the shared loci implicates candidate genes QKI, STK17A, TYR, NSF, and TCF4 likely contribute to the pathophysiology of cataracts and hearing difficulties. In conclusion, this study demonstrates the presence of epidemiologic association between cataract and hearing difficulties and provides new insights into the shared genetic architecture of these two disorders at the common variant level.
MODELS: a six-step framework for developing an infectious disease model
Authors: Rui Jia , Li Kangguo , Wei Hongjie , Guo Xiaohao , Zhao Zeyu , Wang Yao , Song Wentao , Abudunaibi Buasiyamu , Chen Tianmu
Published: April 2024

Since the COVID-19 pandemic began, a plethora of modeling studies related to COVID-19 have been released. While some models stand out due to their innovative approaches, others are flawed in their methodology. To assist novices, frontline healthcare workers, and public health policymakers in navigating the complex landscape of these models, we introduced a structured framework named MODELS. This framework is designed to detail the essential steps and considerations for creating a dependable epidemic model, offering direction to researchers engaged in epidemic modeling endeavors.

Graphical Abstract
Four-year follow-up of psychiatric and psychosomatic profile in patients with Inflammatory Bowel Disease (IBD)
Authors: Gostoli Sara , Ferrara Francesco , Quintavalle Ludovica , Tommasino Sara , Gigante Graziano , Montecchiarini Maria , Urgese Alessia , Guolo Francesco , Subach Regina , D’Oronzo Angelica , Polifemo Annamaria , Buonfiglioli Federica , Cennamo Vincenzo , Rafanelli Chiara
Published: April 2024

Psychological characterization of patients affected by Inflammatory Bowel Disease (IBD) focuses on comorbidity with psychiatric disorders, somatization or alexithymia. Whereas IBD patients had higher risk of stable anxiety and depression for many years after the diagnosis of the disease, there is a lack of studies reporting a comprehensive psychosomatic assessment addressing factors of disease vulnerability, also in the long-term. The objective of this investigation is to fill this gap in the current literature. The aims were thus to assess: a) changes between baseline and a 4-year follow-up in psychiatric diagnoses (SCID), psychosomatic syndromes (DCPR), psychological well-being (PWB-I), lifestyle, gastrointestinal symptoms related to IBD and Irritable Bowel Syndrome (IBS)-like symptoms b) stability of psychiatric and psychosomatic syndromes at 4-year follow-up. A total of 111 IBD outpatients were enrolled; 59.5% of them participated at the follow-up. A comprehensive assessment, including both interviews and self-report questionnaires, was provided at baseline and follow-up. Results showed increased psychiatric diagnoses, physical activity, consumption of vegetables and IBS-like symptoms at follow-up. Additionally, whereas psychiatric diagnoses were no longer present and new psychopathological pictures ensued at follow-up, more than half of the sample maintained psychosomatic syndromes (particularly allostatic overload, type A behavior, demoralization) from baseline to follow-up. Long-term presence/persistence of such psychosocial burden indicates the need for integrating a comprehensive psychosomatic evaluation beyond traditional psychiatric nosography in IBD patients. Moreover, since psychosomatic syndromes represent vulnerability factors of diseases, further studies should target subgroups of patients presenting with persistent psychosomatic syndromes and worse course of the disease.
Tectonic exhumation of a metamorphic core in an arc-continent collision during oblique convergence, Taiwan
Authors: Byrne Timothy , Chojnacki Michael , Lewis Jonathan , Lee Jian-Cheng , Ho Gong-Ruei , Yeh En-Chao , Lee Yuan-Hsi , Tsai Chin-Ho , Evans Mark , Webb Laura
Published: April 2024

Observations over the last few decades from a number of orogenic systems have highlighted the possible importance of tectonic exhumation, i.e., ductile thinning and normal faulting, in exhuming rocks once buried in high-pressure conditions. Taiwan is one of the few active orogens in the world where rocks that once experienced high-pressure metamorphism (> 50 km) are exposed at the Earth’s surface, providing a natural laboratory for advancing our understanding of exhumation processes. We integrate previously published studies of the Taiwan orogen with new structural, geochronological, and fluid inclusion microthermometry data to argue that tectonic extrusion and structural thinning played a critical role in exhuming the metamorphic core of the orogen until very recently, ca. 0.7 Ma. We propose a two-stage process for exhuming the high-pressure metamorphic rocks of the Yuli Belt: an initial stage where exhumation is driven primarily by pressure gradients in a subduction channel and a second stage that is initiated as an orogen-parallel regional-scale strike-slip zone, the Tailuko shear zone, is offset by an orogen-normal strike-slip zone. The offset generates an extensional bend that is filled with extruding high-pressure rocks as the upper crust is structurally thinned. Evidence for tectonic thinning comes primarily from a low-angle penetrative foliation that records significant vertical shortening and a suite of sub-vertical late-stage, mineral-filled veins. Isotopic dating indicates that the second stage started ca. 2.4–3.1 Ma and ended at ca. 0.7 Ma when the northern Backbone Range orocline started to form. We propose the low-angle foliation formed in the footwall of a regional-scale extensional shear zone that rooted to the east, beneath the forearc. Combined tectonic and erosional processes may have limited the topographic growth of the orogen from ~3.0 to < 1.0 Ma.
Aortoesophageal fistula due to esophageal cancer: a case report of successful management
Authors: Saisho Kohei , Mori Naoki , Nakagawa Masashi , Nakamura Eiji , Tanaka Yu , Kaku Hideaki , Tanaka Yuya , Isobe Taro , Otsuka Hiroyuki , Sudo Tomoya , Sakai Hisamune , Ishibashi Nobuya , Hisaka Toru , Tayama Eiki , Fujita Fumihiko
Published: April 2024

Background

Aortoesophageal fistula (AEF) is a rare but potentially life-threatening condition. The best treatment for the AEF due to esophageal carcinoma is still unresolved. Here, we report a rare case of AEF caused by esophageal cancer, that was successfully treated with emergency thoracic endovascular aortic repair (TEVAR), followed by esophagectomy and gastric tube reconstruction.

Case presentation

A 64-year-old man presented with loss of consciousness and hypotension during chemoradiotherapy for advanced esophageal cancer. Enhanced computed tomography showed extravasation from the descending aorta into the esophagus at the tumor site. We performed emergency TEVAR for the AEF, which stabilized the hemodynamics. We then performed thoracoscopic subtotal esophagectomy on day 4 after TEVAR to prevent graft infection, followed by gastric tube reconstruction on day 30 after TEVAR. At 9 months after the onset of AEF, the patient continues to receive outpatient chemotherapy and leads a normal daily life.

Conclusion

TEVAR is a useful hemostatic procedure for AEF. If the patient is in good condition and can continue treatment for esophageal cancer, esophagectomy and reconstruction after TEVAR should be performed to prevent graft infection and maintain quality of life.