bluuebunny/crossref_metadata_2025 huggingface dataset:
A Validation Study of the Revised Caregiving Burden Instrument in Korean Family Caregivers of Stroke Survivors
Authors: Kim So Sun , Roh Young Sook
Published: March 2021

Background: The purpose of the present study was to examine the internal consistency reliability and construct validity of the Caregiving Burden Instrument in Korean informal caregivers of stroke survivors. Methods: A descriptive survey design was used with a convenience sample of 208 primary caregivers of stroke survivors. Internal consistency reliability was assessed using Cronbach’s alpha coefficients. Construct validity was assessed using exploratory and known-group analysis. Results: Each subscale and the total scale demonstrated satisfactory internal consistency reliability. Exploratory factor analysis identified five factors: family support, patient’s dependency, physical health, financial burden, and psychological health, which together accounted for 62.7% of the variance. Known-group analysis indicated that caregivers with more than one year of experience reported significantly higher mean scores for the total burden score and its five subscales compared to those with less than one year. Conclusions: This 23-item instrument demonstrates good internal consistency reliability and construct validity. The tool can be used to effectively assess burden in caregivers of stroke survivors and the data obtained can form the basis for the development of family interventions.
A Feasibility Study of Kinematic Characteristics on the Upper Body According to the Shooting of Elite Disabled Archery Athletes
Authors: Kim Tae-Whan , Lee Jae-Won , Kang Seoung-Ki , Chae Kyu-Yeon , Choi Sang-Hyup , Song Yong-Gwan
Published: March 2021

The purpose of this study is to compare and analyze the kinematic characteristics of the upper limb segments during the archery shooting of Paralympic Wheelchair Class archers (ARW2—second wheelchair class—paraplegia or comparable disability) and Paralympic Standing Class archers (ARST—standing archery class—loss of 25 points in the upper limbs or lower limbs), where archers are classified according to their disability grade among elite disabled archers. The participants of this study were selected as seven elite athletes with disabilities by the ARW2 (n = 4) and ARST (n = 3). The analysis variables were (1) the time required for each phase, (2) the angle of inclination of the body center, (3) the change of trajectory of body center, and (4) the change of the movement trajectory of the bow center by phase when performing six shots in total. The ARW2 group (drawing phase; M = 2.228 s, p < 0.05, holding phase; M = 4.414 s, p < 0.05) showed a longer time than the ARST group (drawing phase; M = 0.985 s, holding phase; M = 3.042 s), and the angle of the body did not show a significant difference between the two groups. Additionally, in the direction of the anteroposterior axis in the drawing phase, the change in the movement trajectory of the body center showed a more significant amount of change in the ARW2 group than in the ARST group, and the change in the movement trajectory of the bow center did not show a significant difference between the two groups.
“The Power of Ethical Leadership”: The Influence of Corporate Social Responsibility on Creativity, the Mediating Function of Psychological Safety, and the Moderating Role of Ethical Leadership
Authors: Kim Byung-Jik , Kim Min-Jik , Kim Tae-Hyun
Published: March 2021

A body of existing literature delves into how corporate social responsibility (CSR) affects employees’ cognition, emotion, and behavior within an organization. These previous studies, however, pay relatively little attention to the influence of CSR on levels of creativity in employees. Considering that creativity is closely related to innovative capability, which is critical for a firm to survive, the relationship between CSR and employees’ creativity and its elaborate underlying processes need further investigation. Based on a group creativity model, we argue that CSR may increase levels of creativity in employees through mediation of enhanced levels of psychological safety in employees. In addition, existing works on CSR have relatively underexplored the contextual role of leadership in translating CSR practices into employees’ attitudes, perceptions, and behaviors. Using three-wave time-lagged survey data from 311 employees in South Korea, we found that CSR enhances employees’ creativity via mediation of psychological safety. Additionally, ethical leadership positively moderates the relationship between CSR and psychological safety. Our findings suggest that psychological safety in employees functions as an important underlying mechanism to describe the CSR–employee creativity link. Furthermore, this paper emphasizes the importance of the moderating role of ethical leadership in the process of CSR activities.
The Impact of the First Wave of the COVID-19 Pandemic on Providing Special Care Dentistry: A Survey for Dentists
Authors: Limeres Posse Jacobo , van Harten Maria T. , Mac Giolla Phadraig Caoimhin , Diniz Freitas Márcio , Faulks Denise , Dougall Alison , Daly Blánaid , Diz Dios Pedro
Published: March 2021

This study aimed to investigate the impact of COVID-19 on the experiences of special care dentistry providers worldwide. An online survey was administered from 10 to 31 July 2020. Age, sex, years of professional activity, COVID-19 status, geographical area of origin and length of lockdown period were recorded for all participating dentists. The relationships between these variables and the changes in clinical activity, the treated patients’ COVID-19 status and the implementation of protective measures in the dental clinic were analyzed. A total of 436 (70.6% women) dentists from 59 countries responded to the survey. Clinical activity was reduced or stopped for 79.1% of respondents. The most common change was to limit treatment to urgent care only (53.7%). Treatment under general anesthesia or deep sedation was discontinued (51.0%) or reduced (35.8%) for the majority of respondents. Male dentists were more likely to maintain their clinical activity than female dentists (p < 0.001), and respondents from North America were more likely to do so than participants from other geographical regions (p < 0.001). Dentists from Latin America and the Caribbean were more likely to report treatment of confirmed cases of COVID-19 than those from Europe (p < 0.001). The implementation of protective measures in the dental office was determined by the survey participant’s sex, intensity of clinical activity and geographical area of origin. To conclude, the provision of special care dentistry was considerably reduced in response to the pandemic. Service maintenance was mainly related to the geographical area in which the surveyed dentists worked, further exacerbating pre-existing inequalities.
Heavy Metal Assessment in Feathers of Eurasian Magpies (Pica pica): A Possible Strategy for Monitoring Environmental Contamination?
Authors: Iemmi Tiziano , Menozzi Alessandro , Pérez-López Marcos , Basini Giuseppina , Grasselli Francesca , Menotta Simonetta , Serventi Paolo , Bertini Simone
Published: March 2021

In the present study, the Eurasian magpie (Pica pica), was evaluated as a possible bioindicator of environmental pollution by heavy metals (HMs). Levels of Ni, Pb, Cd, and Hg in feathers of 64 magpies (31 males and 33 females) were measured by ICP-MS technique. Plasmatic biomarkers of oxidative stress (OS) were also assessed. The birds were captured in the province of Parma (Italy), in different capture sites within 1 km from urban area (UZ), and farther than 5 km from urban area (RZ). Median HM levels were 0.68 mg/kg (0.18–2.27), 2.80 mg/kg (0.41–17.7), <limit of detection (LOD) mg/kg (<LOD–0.25), 3.90 mg/kg (1.35–85.9) for Ni, Pb, Cd and Hg, respectively. No significant differences in HM levels were found according to sex, while Ni and Pb were significantly higher in adult compared to young birds (p = 0.047, p = 0.004). Conversely, Cd and Hg levels in young magpies resulted higher than those of adults (p = 0.001 and p = 0.004). No correlation was found between OS biomarkers and HM levels. No differences were found in HM levels according to capture area, except for Hg level, which resulted higher in magpies of RZ (4.05 mg/kg (1.35–12.7)) compared to UZ (2.99 mg/kg (1.54–85.9)). Further experiments are needed to establish whether magpie feathers could represent a suitable non-invasive tool for biomonitoring HMs in the environment.
Association between High-Sensitive C-Reactive Protein and the Development of Liver Damage in Japanese Male Workers
Authors: Kuroda Reiko , Nogawa Kazuhiro , Watanabe Yuuka , Morimoto Hideki , Sakata Kouichi , Suwazono Yasushi
Published: March 2021

Background: The aim of this study was to determine whether a causative relationship exists between the development of liver damage and increased high-sensitivity C-reactive protein (HsCRP) levels by long-term follow-up in Japanese workers. Methods: The target participants comprised 7830 male workers in a Japanese steel company. The prospective cohort study was performed over a 6-year period, and annual health screening information was analyzed by pooled logistic regression. The endpoint, regarded as the development of liver damage, was defined as aspartate aminotransferase (AST) ≥ 40 IU/L. Results: A significant relationship between the development of liver damage and increased HsCRP levels was observed after adjusting for confounding factors such as various physiological and blood chemistry parameters and lifestyle factors. The odds ratio of a 1.5-fold increase in HsCRP was 1.07 (95% confidence interval: 1.03–1.10, p < 0.001). Conclusions: The results suggested that an increase of HsCRP is associated with the development of liver damage.
Factors Influencing Quality of Life in Early Postpartum Women
Authors: Jeong Yu-Jeong , Nho Ju-Hee , Kim Hye Young , Kim Ji Young
Published: March 2021

Postpartum women experience various changes in their physical and psychological health and in their relationships with their spouse and newborn. This study aimed to identify and evaluate the factors that affect the quality of life (QoL) of women within six weeks after childbirth. A prospective, cross-sectional correlational study was used. A convenience sample of 179 postpartum women was recruited from four postpartum care centers in South Korea. Participants completed structured questionnaires on postpartum fatigue, postpartum depression, marital intimacy, breastfeeding adaptation, and quality of life. Marital intimacy (β = 0.466, p < 0.001) was the most influencing factor on the QoL of women during the postpartum period. In descending order, postpartum fatigue (β = −0.192, p = 0.001), postpartum depression (β = −0.190, p = 0.001), breastfeeding adaptation (β = 0.163, p = 0.002), and occupation (β = 0.163, p = 0.004) all had a significant influence on QoL (F = 32.09, p < 0.001), and the overall explanatory power was 63.6%. It is necessary to assess and consider the physical, psychological, relational, and demographic factors of women during the early postpartum period. Comprehensive interventions need to be developed to improve the QoL of women during the postpartum period.
Diseases Costs and Impact of the Caring Role on Informal Carers of Children with Neuromuscular Disease
Authors: Rodríguez Alicia Aurora , Martínez Óscar , Amayra Imanol , López-Paz Juan Francisco , Al-Rashaida Mohammad , Lázaro Esther , Caballero Patricia , Pérez Manuel , Berrocoso Sarah , García Maitane , Luna Paula María , Pérez-Núñez Paula , Passi Nicole
Published: March 2021

This study aims to evaluate the costs of informal care for children with neuromuscular disease and evaluate how physical and psychological health is associated with socio-demographic variables. A cross sectional design was used with a convenience sample of 110 carers that participated in this study. Participants were recruited from Spanish hospitals and rare diseases organizations. Economic costs and sociodemographic aspects were assessed using the economic costs questionnaire and the sociodemographic questionnaire. Physical and psychological health was evaluated using the CarerQol-7D, PHQ-15, Barthel Index, Zarit Overload Scale and Satisfaction with Life Scale. Carers of children with neuromuscular disease spent a large percentage of their annual income in physical therapy, psychological care and speech therapy. Informal costs differed according to the degree of dependency of the child. These were higher in those caregivers whose child under their care presented low functional independence. The loss of work productivity was related to marital status, use of professional services and the child’s dependency. Finally, carers who were female, single or separated and without a job showed worse physical and psychological health. The results highlighted that carers have to face a number of high costs because of the non-existence of social protection and due to the child’s diagnosis.
Assessing Stability of Crutch Users by Non-Contact Methods
Authors: Vairis Achilles , Brown Suzana , Bess Maurice , Bae Kyu Hyun , Boyack Jonathan
Published: March 2021

Enhancing gait stability in people who use crutches is paramount for their health. With the significant difference in gait compared to users who do not require an assistive device, the use of standard gait analysis tools to measure movement for temporary crush users and physically disabled people proves to be more challenging. In this paper, a novel approach based on video analysis is proposed as non-contact low-cost solution to the more expensive alternative with the data collected from processed videos, two values are calculated: the Signal to Noise Ratio (SNR) of acceleration, and the Signal to Noise Ratio of the jerk (time derivative of acceleration), to assess the user’s stability while they walk with crutches. The adopted methodology has been tested on a total of 10 participants. Five are temporary users of assistive devices with one being a long-term user and the other four novice users, and five are disabled participants who use those assistive devices permanently. Preliminary results show differences between novice users, long-term users, and physically disabled users. The approach is promising and could improve the assessment of crutch user stability, allowing for the correction of gait for individuals while using an inexpensive non-contact setup and preventing unnecessary falls.
Did Expanded Dental Insurance Reduce Out-of-Pocket Expenditures on Dental Care among Older Adults in Korea? Interrupted Time-Series Analysis
Authors: Kim Nam-Hee , Jung Se-Hwan , Kawachi Ichiro
Published: March 2021

The Korean National Health Insurance extended its coverage to reduce the economic burden of receiving dentures and implants for older adults in 2012 and 2014, respectively. We examined whether the new policy resulted in reduced out-of-pocket dental care expenditure in the eligible population, specifically focusing on low-income adults. We used interrupted time-series analysis (ITSA), a quasi-experimental design, to identify the effects of the policy among persons aged 65 or older. Data were extracted from the Korea Health Panel Survey (KHP; 2008–2017). The main outcome was out-of-pocket expenditures on dental care. The ITSA showed that expenditures decreased annually by 4.5% (RR: 0.96, 95% CI: 0.95–0.96) between 2012 and 2014. However, expenditure increased by 7.8% (RR: 1.08, 95% CI: 1.07–1.08) after 2014. Dental insurance coverage did not contribute to reducing the out-of-pocket expenses for dentures among low-income adults, while coverage of dental implants led to an increase in dental expenditure.
Psycho-Electrophysiological Benefits of Forest Therapies Focused on Qigong and Walking with Elderly Individuals
Authors: Yi Jiyune , Kim Seul Gee , Khil Taegyu , Shin Minja , You Jin-Hee , Jeon Sookja , Park Gue Hong , Jeong Ah Young , Lim Youngsuwn , Kim Kahye , Kim Jingun , Kang Byunghoon , Lee Jueun , Park Jeong Hwan , Ku Boncho , Choi Jungmi , Cha Wonseok , Lee Hwa-Jin , Shin Changseob , Shin Wonsop , Kim Jaeuk U.
Published: March 2021

We developed two distinct forest therapy programs (FTPs) and compared their effects on dementia prevention and related health problems for older adults. One was focused on Qigong practice in the forest (QP) and the other involved active walking in the forest (WP). Both FTPs consisted of twelve 2-h sessions over six weeks and were conducted in an urban forest. We obtained data from 25, 18, and 26 participants aged 65 years or above for the QP, WP, and control groups, respectively. Neuropsychological scores via cognition (MoCA), geriatric depression (GDS) and quality of life (EQ-5D), and electrophysiological variables (electroencephalography, bioimpedance, and heart rate variability) were measured. We analyzed the intervention effects with a generalized linear model. Compared to the control group, the WP group showed benefits in terms of neurocognition (increases in the MoCA score, and alpha and beta band power values in the electroencephalogram), sympathetic nervous activity, and bioimpedance in the lower body. On the other hand, the QP group showed alleviated depression and an increased bioimpedance phase angle in the upper body. In conclusion, both active walking and Qigong in the forest were shown to have distinctive neuropsychological and electrophysiological benefits, and both had beneficial effects in terms of preventing dementia and relieving related health problems for elderly individuals.
Improving Vegetable Safety in China: Does Co-Regulation Work?
Authors: Alita Lita , Dries Liesbeth , Oosterveer Peter
Published: March 2021

In the last decade, vegetable safety issues have received growing attention from both consumers and public authorities in China, as vegetable safety hazards pose a serious threat to public health. In 2017, the Industry & Trade Bureau in China implemented a “Market Renovation Program”. This program includes the renovation of wholesale and wet markets, the formal registration of all stallholders in these markets and the introduction of a rapid test for pesticides residues. We apply the co-regulation framework to assess the implementation and results of the renovation program on the safety of vegetables. A mixed methods approach is used to investigate the effects of the renovation program. The qualitative study elaborates on the implementation of the renovation program and the behavioural changes of stakeholders in handling vegetables through interviews and field observations. The quantitative results confirm that the renovation program has a positive impact on vegetable safety. In conclusion, this study shows that the key factor for the success of the renovation program is the transition of authority from the local, public authority to the market management.
Epidemiology of Injuries in First Division Spanish Women’s Soccer Players
Authors: Martín-San Agustín Rodrigo , Medina-Mirapeix Francesc , Esteban-Catalán Andrea , Escriche-Escuder Adrian , Sánchez-Barbadora Mariana , Benítez-Martínez Josep C.
Published: March 2021

The epidemiology of injuries in female soccer has been studied extensively in several national leagues. Even so, data on the first division Spanish league are limited. The objective of this study was to describe the epidemiology of the first division of the Spanish Women’s Soccer League and to analyze data in relation to game position, circumstance, or the moment of injury. Fifteen teams and 123 players participated in the study. Players’ characteristics and their injuries (location, type, diagnosis, circumstance, and moment) were collected. Injuries were described by their frequencies (number and percentage) and incidence rates (IR) with 95% confidence intervals (CIs). Lower limb injuries accounted for 86.8% of total injuries. Anterior cruciate ligament (ACL) and meniscus injuries occurred in totality in non-contact circumstance (0.35/1000 h; 95% CI, 0.18 to 0.62 and 0.23/1000 h; 95% CI, 0.10 to 0.45, respectively). Match injury IRs (19.02/1000 h; 95% CI, 14.89 to 23.97) were significantly higher than training (1.70/1000 h; 95% CI, 1.27 to 2.22). As a conclusion, structures such as the ACL or meniscus are most commonly injured in the non-contact circumstance in the first division of the Spanish Women’s Soccer League. In addition, match situations involve a greater risk of injury than training, increasing the risk to the ankle and knee injuries as the season progresses.
Effect of a Simulated Heat Wave on Physiological Strain and Labour Productivity
Authors: Ioannou Leonidas G. , Mantzios Konstantinos , Tsoutsoubi Lydia , Panagiotaki Zoe , Kapnia Areti K. , Ciuha Ursa , Nybo Lars , Flouris Andreas D. , Mekjavic Igor B.
Published: March 2021

Background: The aim of the study was to investigate the effect of a simulated heat-wave on the labour productivity and physiological strain experienced by workers. Methods: Seven males were confined for ten days in controlled ambient conditions. A familiarisation day was followed by three (pre, during, and post-heat-wave) 3-day periods. During each day volunteers participated in a simulated work-shift incorporating two physical activity sessions each followed by a session of assembly line task. Conditions were hot (work: 35.4 °C; rest: 26.3 °C) during, and temperate (work: 25.4 °C; rest: 22.3 °C) pre and post the simulated heat-wave. Physiological, biological, behavioural, and subjective data were collected throughout the study. Results: The simulated heat-wave undermined human capacity for work by increasing the number of mistakes committed, time spent on unplanned breaks, and the physiological strain experienced by the participants. Early adaptations were able to mitigate the observed implications on the second and third days of the heat-wave, as well as impacting positively on the post-heat-wave period. Conclusions: Here, we show for first time that a controlled simulated heat-wave increases workers’ physiological strain and reduces labour productivity on the first day, but it promotes adaptations mitigating the observed implications during the subsequent days.
Study of Cyberbullying among Adolescents in Recent Years: A Bibliometric Analysis
Authors: Barragán Martín Ana Belén , Molero Jurado María del Mar , Pérez-Fuentes María del Carmen , Simón Márquez María del Mar , Martos Martínez África , Sisto Maria , Gázquez Linares José Jesús
Published: March 2021

In recent years, cyberbullying has been recognized as a severe public health problem and is drawing growing interest. The objective of this study was to perform a bibliometric analysis of the scientific production on adolescent cyberbullying in the last decade. A search for publications was made in the Web of Science database, where the 1530 documents identified were analyzed with BibExcel software and visualized using the Pajek and VOSviewer tools. The predominant language in the publications was English, followed by Spanish. The publication rate was shown to have increased in recent years. The journal “Computers in Human Behavior” had the highest production. The repercussion of new technologies on this phenomenon has been felt, and research groups have enlarged their production in response to the problem. A systematic review and/or meta-analysis examining the contents of the studies identified and the variables related to this problem is therefore necessary. This could identify a point of reference for research in this field and a basis for future reviews of its development and progress over time.
Characteristics and Health Risks of Polycyclic Aromatic Hydrocarbons and Nitro-PAHs in Xinxiang, China in 2015 and 2017
Authors: Zhang Hao , Yang Lu , Zhang Xuan , Xing Wanli , Wang Yan , Bai Pengchu , Zhang Lulu , Li Ying , Hayakawa Kazuichi , Toriba Akira , Tang Ning
Published: March 2021

Fine particulate matter (PM2.5) samples were collected in the summer and winter of 2015 and 2017 in Xinxiang, China. Nine polycyclic aromatic hydrocarbons (PAHs) and three nitro-PAHs (NPAHs) in PM2.5 were detected via high-performance liquid chromatography (HPLC). The PAHs concentration in summer and winter decreased from 6.37 ± 1.30 ng/m3 and 96.9 ± 69.9 ng/m3 to 4.89 ± 2.67 ng/m3 and 49.8 ± 43.4 ng/m3 from 2015 to 2017. NPAHs decreased in winter (from 1707 ± 708 pg/m3 to 1192 ± 1113 pg/m3), but increased in summer from 2015 (336 ± 77.2 pg/m3) to 2017 (456 ± 312 pg/m3). Diagnostic ratios of PAHs indicated that petroleum combustion was the main emission source in summer, and pollutants originating from the combustion of petroleum, coal and biomass dominated in winter. The 2-nitrofluoranthene (2-NFR)/2-nitropyrene (2-NP) ratio in this study demonstrated that the OH radical pathway was the main pathway for the formation of 2-NP and 2-NFR. The mean total benzo[a]pyrene-equivalent concentrations (BaPeq) and incremental lifetime cancer risk (ILCR) values decreased from 2013 to 2017. The high value of total BaPeq in the winter of 2017 in Xinxiang revealed that a high-risk of cancer remained for residents. The results of this study demonstrate that the decreases in PAHs and NPAHS concentrations from 2015 to 2017. Combined with reducing gaseous pollutants concentration, the reduction in this study might be attributable to emissions reductions by implementing the air pollution control regulations in Xinxiang city in 2016.
Psychological Well-Being in Nursing Students: A Multicentric, Cross-Sectional Study
Authors: Reverté-Villarroya Sílvia , Ortega Laura , Raigal-Aran Laia , Sauras-Colón Esther , Ricomà-Muntané Roser , Ballester-Ferrando David , Rascón-Hernán Carolina , Botigué Teresa , Lavedán Ana , González-Osorio Luis , Osorio-Spuler Ximena , Burjalés-Martí Maria Dolors
Published: March 2021

In addition to complying with strict academic standards, nursing students must acquire relevant knowledge and skills, and learn how to carry themselves in different and often stressful professional settings. These obligations could severely affect their mental health. The purpose of this study was to examine the mental health status of undergraduate nursing students and related factors. A total of 1368 nursing students from different universities in Spain and Chile were included in this study, which took place over the 2018–2019 academic year. We assessed their levels of stress related to specific learning methodologies and determined their mental health status using the General Health Questionnaire (GHQ-28). The results revealed that the more advanced the course was, the lower the total GHQ-28 score. The stress generated by different types of training activities had a significant effect on the total GHQ-28 score. These results suggest that nursing education could act as a protective factor against mental health disorders. Although a heavy academic workload could lead to higher levels of stress, overall, it seems that mental health is better in more advanced courses than in initial academic years.
Adolescents’ Bipolar Experiences and Suicide Risk: Well-being and Mental Health Difficulties as Mediators
Authors: Fumero Ascensión , Marrero Rosario J. , Pérez-Albéniz Alicia , Fonseca-Pedrero Eduardo
Published: March 2021

Bipolar disorder is usually accompanied by a high suicide risk. The main aim was to identify the risk and protective factors involved in suicide risk in adolescents with bipolar experiences. Of a total of 1506 adolescents, 467 (31%) were included in the group reporting bipolar experiences or symptoms, 214 males (45.8%) and 253 (54.2%) females. The mean age was 16.22 (SD = 1.36), with the age range between 14 and 19. Suicide risk, behavioral and emotional difficulties, prosocial capacities, well-being, and bipolar experiences were assessed through self-report. Mediation analyses, taking gender as a moderator and controlling age as a covariate, were applied to estimate suicide risk. The results indicated that the effect of bipolar experiences on suicide risk is mediated by behavioral and emotional difficulties rather than by prosocial behavior and subjective well-being. Specifically, emotional problems, problems with peers, behavior problems, and difficulties associated with hyperactivity were the most important variables. This relationship was not modulated by gender. However, the indirect effects of some mediators varied according to gender. These results support the development of suicide risk prevention strategies focused on reducing emotional difficulties, behavioral problems, and difficulties in relationships with others.
Sleep Quality and Associated Factors in Adults with Type 2 Diabetes: A Retrospective Cohort Study
Authors: Kuo Ching-Pyng , Lu Shu-Hua , Huang Chien-Ning , Liao Wen-Chun , Lee Meng-Chih
Published: March 2021

Purpose: Sleep disturbance is one of the major complaints among patients with diabetes. The status of diabetes control and associated complications may contribute to sleep disturbance. This study explored night time sleep and excessive daytime sleepiness in adults with type 2 diabetes and examined the association of diabetes control and associated complications on their sleep quality. Methods: A retrospective cohort study design was used. Type 2 diabetic patients (87 females and 79 males, aged 63.1 ± 10.5 years) were recruited from the outpatient clinics of the endocrine department. Sleep quality was assessed by the Pittsburg Sleep Quality Index and the Epworth Sleepiness Scale. Diabetes control and complications were obtained by retrospectively reviewing patients’ medical records over 1 year prior to study enrollment. Results: 72.3% of recruited patients had poor glycemic control, and 71.1% had at least one diabetic complication. 56.0% of patients experienced poor sleep quality, and 24.1% had excessive daytime sleepiness. Those who were female (OR = 3.45) and who had ophthalmological problems (OR = 3.17) were associated with poor night time sleep quality, but if they did exercise to the point of sweating (OR = 0.48) reduced the risk of poor sleep quality. Furthermore, poor sleep quality (OR = 4.35) and having nephropathy (OR = 3.78) were associated with a higher risk of excessive daytime sleepiness. Conclusions: Sex, ophthalmological problems, nephropathy, and no exercise to the point of sweating are associated with sleep problems in patients with type 2 diabetes. Both lifestyle behaviors and diabetic complications affect sleep disturbances in patients with diabetes.
The Impact of Social Isolation during the COVID-19 Pandemic on Physical and Mental Health: The Lived Experience of Adolescents with Obesity and Their Caregivers
Authors: Pietrabissa Giada , Volpi Clarissa , Bottacchi Michela , Bertuzzi Vanessa , Guerrini Usubini Anna , Löffler-Stastka Henriette , Prevendar Tamara , Rapelli Giada , Cattivelli Roberto , Castelnuovo Gianluca , Molinari Enrico , Sartorio Alessandro
Published: March 2021

Adolescence is a complex developmental phase, made more complex by obesity and the social isolation imposed by the COVID-19 pandemic. The literature related to the impact of social isolation on obesity self-management in adolescents is scant and inconsistent. This paper describes the phenomenon from the perspectives of a sample of adolescents with obesity enrolled in an inpatients’ multidisciplinary rehabilitation program for weight-loss and their caregivers, and its impact on different life domains. Individual semi-structured ad hoc interviews were conducted with 10 adolescent-caregiver dyads, and narratives were qualitatively investigated using an interpretative phenomenology approach to data. Twenty participants took part in the study. The major themes that emerged from this study fall into five basic categories: (1) COVID-19 as an opportunity to reconsider what makes a good life; (2) Persistence in life; (3) Empowering relationship; (4) Daily routine in quarantine; (5) Lives on hold. Understandings drawn from this study may assist health care professionals in providing holistic support, and guidance to adolescents with weight-related issues and their caregivers who experience social isolation during the COVID-19 pandemic.
PLD-SLAM: A New RGB-D SLAM Method with Point and Line Features for Indoor Dynamic Scene
Authors: Zhang Chenyang , Huang Teng , Zhang Rongchun , Yi Xuefeng
Published: March 2021

RGB-D SLAM (Simultaneous Localization and Mapping) generally performs smoothly in a static environment. However, in dynamic scenes, dynamic features often cause wrong data associations, which degrade accuracy and robustness. To address this problem, in this paper, a new RGB-D dynamic SLAM method, PLD-SLAM, which is based on point and line features for dynamic scenes, is proposed. First, to avoid under-over segmentation caused by deep learning, PLD-SLAM combines deep learning for semantic information segmentation with the K-Means clustering algorithm considering depth information to detect the underlying dynamic features. Next, two consistency check strategies are utilized to check and filter out the dynamic features more reasonably. Then, to obtain a better practical performance, point and line features are utilized to calculate camera pose in the dynamic SLAM, which is also different from most published dynamic SLAM algorithms based merely on point features. The optimization model with point and line features is constructed and utilized to calculate the camera pose with higher accuracy. Third, enough experiments on the public TUM RGB-D dataset and the real-world scenes are conducted to verify the location accuracy and performance of PLD-SLAM. We compare our experimental results with several state-of-the-art dynamic SLAM methods in terms of average localization errors and the visual difference between the estimation trajectories and the ground-truth trajectories. Through the comprehensive comparisons with these dynamic SLAM schemes, it can be fully demonstrated that PLD-SLAM can achieve comparable or better performances in dynamic scenes. Moreover, the feasibility of camera pose estimation based on both point features and line features has been proven by the corresponding experiments from a comparison with our proposed PLD-SLAM only based on point features.
The Role of Spatio-Temporal Information to Govern the COVID-19 Pandemic: A European Perspective
Authors: Müller Hartmut , Louwsma Marije
Published: March 2021

The Covid-19 pandemic put a heavy burden on member states in the European Union. To govern the pandemic, having access to reliable geo-information is key for monitoring the spatial distribution of the outbreak over time. This study aims to analyze the role of spatio-temporal information in governing the pandemic in the European Union and its member states. The European Nomenclature of Territorial Units for Statistics (NUTS) system and selected national dashboards from member states were assessed to analyze which spatio-temporal information was used, how the information was visualized and whether this changed over the course of the pandemic. Initially, member states focused on their own jurisdiction by creating national dashboards to monitor the pandemic. Information between member states was not aligned. Producing reliable data and timeliness reporting was problematic, just like selecting indictors to monitor the spatial distribution and intensity of the outbreak. Over the course of the pandemic, with more knowledge about the virus and its characteristics, interventions of member states to govern the outbreak were better aligned at the European level. However, further integration and alignment of public health data, statistical data and spatio-temporal data could provide even better information for governments and actors involved in managing the outbreak, both at national and supra-national level. The Infrastructure for Spatial Information in Europe (INSPIRE) initiative and the NUTS system provide a framework to guide future integration and extension of existing systems.
Multiple Sclerosis-Associated hnRNPA1 Mutations Alter hnRNPA1 Dynamics and Influence Stress Granule Formation
Authors: Clarke Joseph-Patrick W. E. , Thibault Patricia A. , Salapa Hannah E. , Kim David E. , Hutchinson Catherine , Levin Michael C.
Published: March 2021

Evidence indicates that dysfunctional heterogeneous ribonucleoprotein A1 (hnRNPA1; A1) contributes to the pathogenesis of neurodegeneration in multiple sclerosis. Understanding molecular mechanisms of neurodegeneration in multiple sclerosis may result in novel therapies that attenuate neurodegeneration, thereby improving the lives of MS patients with multiple sclerosis. Using an in vitro, blue light induced, optogenetic protein expression system containing the optogene Cryptochrome 2 and a fluorescent mCherry reporter, we examined the effects of multiple sclerosis-associated somatic A1 mutations (P275S and F281L) in A1 localization, cluster kinetics and stress granule formation in real-time. We show that A1 mutations caused cytoplasmic mislocalization, and significantly altered the kinetics of A1 cluster formation/dissociation, and the quantity and size of clusters. A1 mutations also caused stress granule formation to occur more quickly and frequently in response to blue light stimulation. This study establishes a live cell optogenetics imaging system to probe localization and association characteristics of A1. It also demonstrates that somatic mutations in A1 alter its function and promote stress granule formation, which supports the hypothesis that A1 dysfunction may exacerbate neurodegeneration in multiple sclerosis.
Targeting Intercellular Communication in the Bone Microenvironment to Prevent Disseminated Tumor Cell Escape from Dormancy and Bone Metastatic Tumor Growth
Authors: Kreps Lauren M. , Addison Christina L.
Published: March 2021

Metastasis to the bone is a common feature of many cancers including those of the breast, prostate, lung, thyroid and kidney. Once tumors metastasize to the bone, they are essentially incurable. Bone metastasis is a complex process involving not only intravasation of tumor cells from the primary tumor into circulation, but extravasation from circulation into the bone where they meet an environment that is generally suppressive of their growth. The bone microenvironment can inhibit the growth of disseminated tumor cells (DTC) by inducing dormancy of the DTC directly and later on following formation of a micrometastatic tumour mass by inhibiting metastatic processes including angiogenesis, bone remodeling and immunosuppressive cell functions. In this review we will highlight some of the mechanisms mediating DTC dormancy and the complex relationships which occur between tumor cells and bone resident cells in the bone metastatic microenvironment. These inter-cellular interactions may be important targets to consider for development of novel effective therapies for the prevention or treatment of bone metastases.
miRNA Mediated Regulation and Interaction between Plants and Pathogens
Authors: Yang Xiaoqian , Zhang Lichun , Yang Yuzhang , Schmid Markus , Wang Yanwei
Published: March 2021

Plants have evolved diverse molecular mechanisms that enable them to respond to a wide range of pathogens. It has become clear that microRNAs, a class of short single-stranded RNA molecules that regulate gene expression at the transcriptional or post-translational level, play a crucial role in coordinating plant-pathogen interactions. Specifically, miRNAs have been shown to be involved in the regulation of phytohormone signals, reactive oxygen species, and NBS-LRR gene expression, thereby modulating the arms race between hosts and pathogens. Adding another level of complexity, it has recently been shown that specific lncRNAs (ceRNAs) can act as decoys that interact with and modulate the activity of miRNAs. Here we review recent findings regarding the roles of miRNA in plant defense, with a focus on the regulatory modes of miRNAs and their possible applications in breeding pathogen-resistance plants including crops and trees. Special emphasis is placed on discussing the role of miRNA in the arms race between hosts and pathogens, and the interaction between disease-related miRNAs and lncRNAs.
Molecular Mechanisms and Tumor Biological Aspects of 5-Fluorouracil Resistance in HCT116 Human Colorectal Cancer Cells
Authors: Kurasaka Chinatsu , Ogino Yoko , Sato Akira
Published: March 2021

5-Fluorouracil (5-FU) is a cornerstone drug used in the treatment of colorectal cancer (CRC). However, the development of resistance to 5-FU and its analogs remain an unsolved problem in CRC treatment. In this study, we investigated the molecular mechanisms and tumor biological aspects of 5-FU resistance in CRC HCT116 cells. We established an acquired 5-FU-resistant cell line, HCT116RF10. HCT116RF10 cells were cross-resistant to the 5-FU analog, fluorodeoxyuridine. In contrast, HCT116RF10 cells were collaterally sensitive to SN-38 and CDDP compared with the parental HCT16 cells. Whole-exome sequencing revealed that a cluster of genes associated with the 5-FU metabolic pathway were not significantly mutated in HCT116 or HCT116RF10 cells. Interestingly, HCT116RF10 cells were regulated by the function of thymidylate synthase (TS), a 5-FU active metabolite 5-fluorodeoxyuridine monophosphate (FdUMP) inhibiting enzyme. Half of the TS was in an active form, whereas the other half was in an inactive form. This finding indicates that 5-FU-resistant cells exhibited increased TS expression, and the TS enzyme is used to trap FdUMP, resulting in resistance to 5-FU and its analogs.
A Holistic Evolutionary and 3D Pharmacophore Modelling Study Provides Insights into the Metabolism, Function, and Substrate Selectivity of the Human Monocarboxylate Transporter 4 (hMCT4)
Authors: Papakonstantinou Eleni , Vlachakis Dimitrios , Thireou Trias , Vlachoyiannopoulos Panayiotis G. , Eliopoulos Elias
Published: March 2021

Monocarboxylate transporters (MCTs) are of great research interest for their role in cancer cell metabolism and their potential ability to transport pharmacologically relevant compounds across the membrane. Each member of the MCT family could potentially provide novel therapeutic approaches to various diseases. The major differences among MCTs are related to each of their specific metabolic roles, their relative substrate and inhibitor affinities, the regulation of their expression, their intracellular localization, and their tissue distribution. MCT4 is the main mediator for the efflux of L-lactate produced in the cell. Thus, MCT4 maintains the glycolytic phenotype of the cancer cell by supplying the molecular resources for tumor cell proliferation and promotes the acidification of the extracellular microenvironment from the co-transport of protons. A promising therapeutic strategy in anti-cancer drug design is the selective inhibition of MCT4 for the glycolytic suppression of solid tumors. A small number of studies indicate molecules for dual inhibition of MCT1 and MCT4; however, no selective inhibitor with high-affinity for MCT4 has been identified. In this study, we attempt to approach the structural characteristics of MCT4 through an in silico pipeline for molecular modelling and pharmacophore elucidation towards the identification of specific inhibitors as a novel anti-cancer strategy.
Preclinical Investigation of Trifluoperazine as a Novel Therapeutic Agent for the Treatment of Pulmonary Arterial Hypertension
Authors: Grobs Yann , Awada Charifa , Lemay Sarah-Eve , Romanet Charlotte , Bourgeois Alice , Toro Victoria , Nadeau Valérie , Shimauchi Kana , Orcholski Mark , Breuils-Bonnet Sandra , Tremblay Eve , Provencher Steeve , Paulin Roxane , Boucherat Olivier , Bonnet Sébastien
Published: March 2021

Trifluoperazine (TFP), an antipsychotic drug approved by the Food and Drug Administration, has been show to exhibit anti-cancer effects. Pulmonary arterial hypertension (PAH) is a devastating disease characterized by a progressive obliteration of small pulmonary arteries (PAs) due to exaggerated proliferation and resistance to apoptosis of PA smooth muscle cells (PASMCs). However, the therapeutic potential of TFP for correcting the cancer-like phenotype of PAH-PASMCs and improving PAH in animal models remains unknown. PASMCs isolated from PAH patients were exposed to different concentrations of TFP before assessments of cell proliferation and apoptosis. The in vivo therapeutic potential of TFP was tested in two preclinical models with established PAH, namely the monocrotaline and sugen/hypoxia-induced rat models. Assessments of hemodynamics by right heart catheterization and histopathology were conducted. TFP showed strong anti-survival and anti-proliferative effects on cultured PAH-PASMCs. Exposure to TFP was associated with downregulation of AKT activity and nuclear translocation of forkhead box protein O3 (FOXO3). In both preclinical models, TFP significantly lowered the right ventricular systolic pressure and total pulmonary resistance and improved cardiac function. Consistently, TFP reduced the medial wall thickness of distal PAs. Overall, our data indicate that TFP could have beneficial effects in PAH and support the view that seeking new uses for old drugs may represent a fruitful approach.
Pertussis Toxin Inhibits Encephalitogenic T-Cell Infiltration and Promotes a B-Cell-Driven Disease during Th17-EAE
Authors: Maria Zahra , Turner Emma , Agasing Agnieshka , Kumar Gaurav , Axtell Robert C.
Published: March 2021

Pertussis toxin (PTX) is a required co-adjuvant for experimental autoimmune encephalomyelitis (EAE) induced by immunization with myelin antigen. However, PTX’s effects on EAE induced by the transfer of myelin-specific T helper cells is not known. Therefore, we investigated how PTX affects the Th17 transfer EAE model (Th17-EAE). We found that PTX significantly reduced Th17-EAE by inhibiting chemokine-receptor-dependent trafficking of Th17 cells. Strikingly, PTX also promoted the accumulation of B cells in the CNS, suggesting that PTX alters the disease toward a B-cell-dependent pathology. To determine the role of B cells, we compared the effects of PTX on Th17-EAE in wild-type (WT) and B-cell-deficient (µMT) mice. Without PTX treatment, disease severity was equivalent between WT and µMT mice. In contrast, with PTX treatment, the µMT mice had significantly less disease and a reduction in pathogenic Th17 cells in the CNS compared to the WT mice. In conclusion, this study shows that PTX inhibits the migration of pathogenic Th17 cells, while promoting the accumulation of pathogenic B cells in the CNS during Th17-EAE. These data provide useful methodological information for adoptive-transfer Th17-EAE and, furthermore, describe another important experimental system to study the pathogenic mechanisms of B cells in multiple sclerosis.
3D Environment Is Required In Vitro to Demonstrate Altered Bone Metabolism Characteristic for Type 2 Diabetics
Authors: Häussling Victor , Aspera-Werz Romina H. , Rinderknecht Helen , Springer Fabian , Arnscheidt Christian , Menger Maximilian M. , Histing Tina , Nussler Andreas K. , Ehnert Sabrina
Published: March 2021

A large British study, with almost 3000 patients, identified diabetes as main risk factor for delayed and nonunion fracture healing, the treatment of which causes large costs for the health system. In the past years, much progress has been made to treat common complications in diabetics. However, there is still a lack of advanced strategies to treat diabetic bone diseases. To develop such therapeutic strategies, mechanisms leading to massive bone alterations in diabetics have to be well understood. We herein describe an in vitro model displaying bone metabolism frequently observed in diabetics. The model is based on osteoblastic SaOS-2 cells, which in direct coculture, stimulate THP-1 cells to form osteoclasts. While in conventional 2D cocultures formation of mineralized matrix is decreased under pre-/diabetic conditions, formation of mineralized matrix is increased in 3D cocultures. Furthermore, we demonstrate a matrix stability of the 3D carrier that is decreased under pre-/diabetic conditions, resembling the in vivo situation in type 2 diabetics. In summary, our results show that a 3D environment is required in this in vitro model to mimic alterations in bone metabolism characteristic for pre-/diabetes. The ability to measure both osteoblast and osteoclast function, and their effect on mineralization and stability of the 3D carrier offers the possibility to use this model also for other purposes, e.g., drug screenings.