bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular detection of Bartonella species in wild small mammals in western Yunnan Province, China
Authors: Luo Yun-Yan , Yu Dan , Zhang Hong-Ze , Liu Zheng-Xiang , Hong Ru-Dan , Hong Mei , Ai Zhi-Qiong , Zhu Jun-Jie , Yin Jia-Xiang
Published: November 2023

Background

Small mammals serve as the main reservoir for Bartonella and as a proxy indicator of the potential risk of Bartonella transmission from nature to humans. They offer a valuable early warning for human infection. Nevertheless, geographical variations in the impact of the host on the occurrence of Bartonella infection are underestimated. This study was designed to investigate the infection characteristics of Bartonella and explore its species diversity in wild small mammals in western Yunnan Province, China.

Methods

Wild small mammals were captured from Yulong, Jianchuan, and Lianghe counties in western Yunnan Province between 2015 and 2016. Real-time quantitative PCR (qPCR) was used to detect Bartonella infection, and the Bartonella species were identified by phylogenetic analysis. The factors associated with Bartonella infection in small mammals were analyzed by the Chi-square Test.

Results

The prevalence of Bartonella in small mammals was 47.85% (768/1605). Lianghe County had the highest Bartonella infection rate, with 56.27% of the samples tested positive, followed by a rate of 50.91% was tested in Yulong County, and 39.97% in Jianchuan County (p < 0.001). Bartonella was detected positive in a total 25 small mammal species, with infection rates ranging from 2.17% to 100%. Niviventer fulvescens had the highest Bartonella infection rate. In comparison with the dominant small mammal species, Eothenomys mileyus had the lowest Bartonella infection rate than that in Apodemus chevrieri, Rattus tanezumi, and Apodemus draco (p < 0.001). Male small mammals had a higher infection rate than females (p < 0.05). The prevalence of Bartonella in small mammals during the summer season was higher compared to the other three seasons (p < 0.001). Woodland landscape had the highest Bartonella infection rate (p < 0.001). Bartonella rochalimae, B. japonica, B. tribocorum, B. washoensis, B. sylvatica, and B. rattimassiliensis were obtained from infected small mammals.

Conclusion

This study showed a high prevalence of Bartonella was detected with various Bartonella species in small mammals in Yulong, Jianchuan, and Lianghe counties of western Yunnan Province. These findings hold significant scientific clues, providing valuable reference points for further research of Bartonella natural foci in Yunnan or other analogues environments.
Opportunities to Breed Diverse Sweetpotato Varieties for California Organic Production
Authors: Parker Travis , Leach Kristyn , Stoddard C. Scott , Roser Laura , Palkovic Antonia , Williams Troy , Lo Sassoum , Gepts Paul , La Bonte Don , Chung Ga Young , Brummer E. Charles
Published: November 2023

Sweetpotatoes are a major crop in California, ranking sixth in value among organic commodities in the state. In recent years, there has been growing consumer interest in diverse specialty varieties, particularly purple types and those associated with Asian American and Pacific Islander (AAPI) communities, some of which are currently imported into the state. In this study, we screened 45 diverse sweetpotato varieties and breeding lines under California organic conditions in a preliminary characterization of their agronomic performance. We then conducted culinary evaluations with a tasting panel of students primarily identifying as Asian/Asian American to determine the preference for each type in terms of flavor and culinary appeal. Our results indicated that major tradeoffs exist among existing germplasm, with no variety or line excelling across all agronomic and culinary traits. These results suggest that sweetpotato breeding could be an effective mechanism to combine superior agronomic traits of major commercial classes with the high culinary quality of diverse materials that are not adapted to California organic production. These results provide a strong justification for the value of sweetpotato breeding to ultimately promote a more profitable, sustainable, and just food system in the region.
Phytobiotic-Prebiotic Feed Additive Containing a Combination of Carob Pulp, Chicory, and Fenugreek Improve Growth Performance, Carcass Traits, and Fecal Microbiota of Fattening Pigs
Authors: Juhász Ákos , Molnár-Nagy Viviána , Bata Zsófia , Tso Ko-Hua , Posta Katalin
Published: November 2023

The purpose of this study was to determine the effectiveness of a phytobiotic-prebiotic feed additive (PPFA, which contains a combination of chicory and extracts of carob pulp and fenugreek) in the diets of fattening pigs on growth indicators, carcass characteristics, and fecal microbiota. A total of 329 crossbred pigs were randomly divided into two dietary treatments, including a basal diet without additives as the control group and a basal diet supplemented with 1 kg/T PPFA as the trial group. The PPFA supplementation led to a significant increase in the body weight gain and average daily gain of the trial group compared to those of the control group after 70 days of feeding. Through the S-EUROP evaluation system, we also found that the fattening pigs fed PPFA significantly improved their carcass indicators. Furthermore, it was shown that PPFA regulated porcine intestinal microbiota, including promoting the growth of the beneficial commensal bacteria (i.e., Bifidobacterium and Lactobacillus) while inhibiting some potential pathogen bacteria (i.e., Bacteroidaceae and Campylobacteraceae). Our work revealed that the phytobiotic-prebiotic feed additive containing carob pulp, chicory, and fenugreek positively influences the intestinal microbiota, growth performance, and carcass traits in fattening swine.
Genome-Wide Association Study Reveals Novel Loci Associated with Body Conformation Traits in Qinchuan Cattle
Authors: Yu Hengwei , Yu Shengchen , Guo Juntao , Cheng Gong , Mei Chugang , Zan Linsen
Published: November 2023

A genome-wide association study (GWAS) is an effective tool for identifying the dominant genes of complex economic traits in livestock by statistical analysis of genotype data and measured phenotype data. In this study, we rigorously measured 14 body conformation traits in 254 Qinchuan cattle, comprising body weight (BW), body height (BOH), back height (BAH), buttock height (BUH), chest depth (CD), chest width (CW), hip cross height (HCH), body length (BL), hip width (HW), rump length (RL), pin bone width (PBW), chest girth (CG), abdomen circumference (AG), and calf circumference (CC). After quality control, 281,889 SNPs were generated for GWAS with different traits. A total of 250 suggestive SNPs (p < 3.54 × 10−6) were screened and 37 candidate genes were annotated. Furthermore, we performed a linkage disequilibrium analysis of SNP loci and considered published studies, identifying the eight genes (ADAMTS17, ALDH1A3, CHSY1, MAGEL2, MEF2A, SYNM, CNTNAP5, and CTNNA3) most likely to be involved in growth traits. This study provides new insights into the regulatory mechanisms of bovine body size development, which can be very useful in the development of management and breeding strategies.
Recent Northward Expansion of a Passerine Bird Species, Brownish-Flanked Bush Warbler (Horornis fortipes)
Authors: Zhang Qianyi , Alström Per , Xia Canwei
Published: November 2023

Northward expansions of bird distributions have been commonly observed in the Northern Hemisphere, likely as a result of climate change. The causes and ecological impacts of such range shifts have received extensive attention, but studies on the process of range shifts are still relatively scarce. The Brownish-flanked Bush Warbler (Horornis fortipes) has expanded northward from 35° N to 40° N during the past decade. In this study, we collated 77 records of the species beyond its traditional distribution during the past ten years from citizen science data. Most of the new records were from northeast of its traditional distribution, including the North China Plain, Taihang Mountains, and Taishan Mountain, and a few records from the northern margin of the Qinling Mountains and Qinghai–Tibet Plateau. We concluded that the Brownish-flanked Bush Warbler has bred in this new area in at least six sites. The newly established populations are assumed to belong to the subspecies H. f. davidianus, which can be divided into eastern and western dialect groups based on differences in songs. Song recordings from 10 males from Beijing and its adjacent areas were collected. Bayesian analysis based on the acoustic traits indicated that these males were most likely from the western dialect area, with a posterior probability of 99.975%. Combining topographical data with the habitat preference of the species, we inferred that these individuals spread northeastward from the Qinling Mountains to Taihang Mountains, and further along the Yanshan Mountains. This study is a case study of the distribution expansion of a bird species, which reflects the dynamics of a species in the early stage of its northward expansion.
Editorial for the Special Issue “Antiprotozoal Activity of Natural Products”
Authors: Baldassarri Cecilia , Spinozzi Eleonora , Ferrati Marta , Rossi Paolo , Maggi Filippo , Petrelli Riccardo
Published: November 2023

Neglected tropical diseases (NTDs), a diverse group of infectious diseases, represent the leading cause of morbidity and mortality among the world’s low-income populations [...]
Benzothiazole-Phthalimide Hybrids as Anti-Breast Cancer and Antimicrobial Agents
Authors: Barbarossa Alexia , Ceramella Jessica , Carocci Alessia , Iacopetta Domenico , Rosato Antonio , Limongelli Francesco , Carrieri Antonio , Bonofiglio Daniela , Sinicropi Maria Stefania
Published: November 2023

The benzothiazole nucleus is a major heterocyclic scaffold whose therapeutic potential has been thoroughly explored due to its structural simplicity and ease of synthesis. In fact, several benzothiazole derivatives have been synthesized over time, demonstrating numerous pharmacological properties such as anticancer, antimicrobial, anti-inflammatory, and antioxidant activities. Herein, we propose a new series of benzothiazole-phthalimide hybrids obtained by linking the phthalimide moiety to differently substituted benzothiazole nuclei through the N atom. These compounds have been screened for their anticancer properties against two human breast cancer cell lines. Furthermore, we delved into the mechanism of action of the most active hybrid, compound 3h, by assessing its capability to damage the nuclear DNA, trigger the apoptotic process in the high metastatic MDA-MB-231 cells, and prevent cellular migration. Moreover, in view of the documented antimicrobial activities of the two scaffolds involved, we explored the antibacterial and antifungal effects of the studied compounds by means of the broth microdilution method. Among the studied compounds, 3h showed the highest antimicrobial activity, both against gram-positive and gram-negative bacterial strains belonging to the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) and against fungal strains of the Candida species with MICs values ranging from 16 to 32 µg/mL.
Assessing the Effect of Oxytetracycline on the Selection of Resistant Escherichia coli in Treated and Untreated Broiler Chickens
Authors: Pokrant Ekaterina , Vargas María Belén , Navarrete María José , Yévenes Karina , Trincado Lina , Cortés Paula , Maddaleno Aldo , Lapierre Lisette , Cornejo Javiera
Published: November 2023

Oxytetracycline (OTC) is administered in the poultry industry for the treatment of digestive and respiratory diseases. The use of OTC may contribute to the selection of resistant bacteria in the gastrointestinal tract of birds or in the environment. To determine the effect of OTC on the selection of resistant Escherichia coli strains post-treatment, bacteria were isolated from droppings and litter sampled from untreated and treated birds. Bacterial susceptibility to tetracyclines was determined by the Kirby–Bauer test. A total of 187 resistant isolates were analyzed for the presence of tet(A), (B), (C), (D), (E), and (M) genes by PCR. Fifty-four strains were analyzed by PFGE for subtyping. The proportion of tetracycline-resistant E. coli strains isolated was 42.88%. The susceptibility of the strains was treatment-dependent. A high clonal diversity was observed, with the tet(A) gene being the most prevalent, followed by tet(C). Even at therapeutic doses, there is selection pressure on resistant E. coli strains. The most prevalent resistance genes were tet(A) and tet(C), which could suggest that one of the main mechanisms of resistance of E. coli to tetracyclines is through active efflux pumps.
Biogenic Selenium Nanoparticles Synthesized Using Alginate Oligosaccharides Attenuate Heat Stress-Induced Impairment of Breast Meat Quality via Regulating Oxidative Stress, Metabolome and Ferroptosis in Broilers
Authors: Yang Yu-Ying , An Yu-Chen , Zhang Shu-Yue , Huang Meng-Yi , Ye Xue-Qing , Zhao Zhi-Hui , Liu Wen-Chao
Published: November 2023

Selenium (Se) is an indispensable trace element with versatile functions in antioxidant defense in poultry. In our previous study, we synthesized a novel type of biogenic selenium nanoparticle based on alginate oligosaccharides (SeNPs-AOS), and found that the particles are sized around 80 nm with an 8% Se content, and the dietary addition of 5 mg/kg of SeNPs-AOS could effectively alleviate the deleterious effects of heat stress (HS) in broilers, but it is still unclear whether SeNPs-AOS can improve the meat quality. Therefore, the aim of this study was to evaluate the protective effects of SeNPs-AOS on breast meat quality in heat-stressed broilers, and explore the relevant mechanisms. Birds at the age of 21 days were randomly divided into four groups with six replicates per group (eight broilers per replicate) according to a 2 × 2 experimental design, using HS (33 ± 2 °C, 10 h/day vs. thermoneutral, TN, under 23 ± 1.5 °C) and SeNPs-AOS (5 mg/kg feed vs. no inclusion) as variables. The results showed that dietary SeNPs-AOS decreased the cooking loss (p < 0.05), freezing loss (p < 0.001), and shear force (p < 0.01) of breast muscle in heat-stressed broilers. The non-targeted metabolomics analysis of the breast muscle identified 78 differential metabolites between the HS and HS + SeNPs-AOS groups, mainly enriched in the arginine and proline metabolism, β-alanine metabolism, D-arginine and D-ornithine metabolism, pantothenate, and CoA biosynthesis pathways (p < 0.05). Meanwhile, supplementation with SeNPs-AOS increased the levels of the total antioxidant capacity (T-AOC), the activities of catalase (CAT) and glutathione peroxidase (GSH-Px), and decreased the content of malondialdehyde (MDA) in the breast muscle (p < 0.05) in broilers under HS exposure. Additionally, SeNPs-AOS upregulated the mRNA expression of CAT, GPX1, GPX3, heme oxygenase-1 (HO-1), masculoaponeurotic fibrosarcoma G (MafG), MafK, selenoprotein W (SELENOW), SELENOK, ferritin heavy polypeptide-1 (FTH1), Ferroportin 1 (Fpn1), and nuclear factor erythroid 2-related factor 2 (Nrf2) (p < 0.05), while it downregulated Kelch-like ECH-associated pro-36 tein 1 (Keap1) and prostaglandin-endoperoxide Synthase 2 (PTGS2) expression (p < 0.05) in broilers under HS. These findings demonstrated that the dietary addition of SeNPs-AOS mitigated HS-induced oxidative damage and metabolite changes in the breast muscle of broilers, which may be related to the regulation of the Nrf2 signaling pathway and selenoprotein synthesis. In addition, SeNPs-AOS upregulated the breast muscle gene expression of anti-ferroptosis-related molecules in broilers under HS, suggesting that SeNPs-AOS can be used as novel Se supplements against HS in broilers.
Unmasking Nasality to Assess Hypernasality
Authors: Moreno-Torres Ignacio , Lozano Andrés , Bermúdez Rosa , Pino Josué , Méndez María Dolores García , Nava Enrique
Published: November 2023

Automatic evaluation of hypernasality has been traditionally computed using monophonic signals (i.e., combining nose and mouth signals). Here, this study aimed to examine if nose signals serve to increase the accuracy of hypernasality evaluation. Using a conventional microphone and a Nasometer, we recorded monophonic, mouth, and nose signals. Three main analyses were performed: (1) comparing the spectral distance between oral/nasalized vowels in monophonic, nose, and mouth signals; (2) assessing the accuracy of Deep Neural Network (DNN) models in classifying oral/nasal sounds and vowel/consonant sounds trained with nose, mouth, and monophonic signals; (3) analyzing the correlation between DNN-derived nasality scores and expert-rated hypernasality scores. The distance between oral and nasalized vowels was the highest in the nose signals. Moreover, DNN models trained on nose signals outperformed in nasal/oral classification (accuracy: 0.90), but were slightly less precise in vowel/consonant differentiation (accuracy: 0.86) compared to models trained on other signals. A strong Pearson’s correlation (0.83) was observed between nasality scores from DNNs trained with nose signals and human expert ratings, whereas those trained on mouth signals showed a weaker correlation (0.36). We conclude that mouth signals partially mask the nasality information carried by nose signals. Significance: the accuracy of hypernasality assessment tools may improve by analyzing nose signals.
Air-Stable Near-Infrared Sensitive Organic Phototransistors Realized via Tri-Layer Planar Heterojunction
Authors: Hu Yao , Wang Yongshun , Li Yao , Zhang Lijun
Published: November 2023

Near-infrared (NIR) light has many applications in agriculture, transportation, medicine, the military, and other fields. Lead phthalocyanine (PbPc) exhibits excellent near-infrared (NIR) light absorption characteristics and is widely used in NIR-sensitive organic photodetectors. In this work, PbPc-based NIR organic phototransistors (OPTs) with different active layer structures were designed and fabricated. The photo-absorption characteristics of organic films, photosensitive properties, and air stability of the devices were investigated. The results suggested that (i) the bilayer planar heterojunction (PHJ) devices exhibit far better photosensitive performance than the single layer ones due to higher mobility of the formers than the latters; (ii) the bilayer PHJ ones with p-type channel have equivalent photosensitive performance to those with n-type channel owing to equivalent mobility, higher NIR absorption and lower exciton dissociation efficiency of the formers than the latters; (iii) the bilayer PHJ ones with p-type channel possess superior air stability to those with n-type channel thanks to better air stability of pentacene channel layer than C60 channel layer; (iv) the tri-layer PHJ ones perform better than the bilayer PHJ ones with p-type channel and exhibit a high photoresponsivity of 1415 mA/W and a maximum photo-to-dark current ratio of 1.2 × 104, and such an outstanding performance benefits from the virtues of tri-layer PHJ structure including high light absorption, carrier mobility and exciton dissociation efficiency; and (v) the air stability of the tri-layer PHJ ones is better than that of the bilayer PHJ ones with p-type channel, which can be attributed to the passivation of the top-level C60 layer.
Spatial and Temporal Changes of Typical Vegetation in the Yellow River Delta Based on Zhuhai-1 Hyperspectral Data
Authors: Jiang Junyi , Tian Hao , Fu Pingjie , Meng Fei , Tong Hongju
Published: November 2023

The Yellow River Delta wetland boasts a diverse range of vegetation species and harbors an ecosystem that is both sensitive and fragile, so it is of great practical significance to accurately extract the vegetation information and analyze the spatial and temporal changes of this region. Based on the hyperspectral image data of Zhuhai-1, this study selected the characteristic bands through the continuum removal method. It combined these spectral attributes with index-based characteristics, utilizing the random forest algorithm to classify prevalent vegetation types while subjecting the outcomes to thorough analysis. It was shown that (1) when integrating spectral features, red edge indices, water indices, and vegetation indices to classify five distinct vegetation types in the Yellow River Delta during 2020 and 2022, the random forest classification algorithm showed higher classification accuracy, and the study achieved commendable overall classification accuracy rates and Kappa coefficients of 85.92% and 0.84, and 86.25% and 0.84, respectively. (2) In 2020 and 2022, the distribution of vegetation in the Yellow River Delta exhibited the following order: Suaeda glauca > Phragmites > Spartina alterniflora > Tamarisk > Typha orientalis Presl. With the exception of Spartina alterniflora, all categories of vegetation witnessed an increase in their distribution areas. Phragmites experienced the most significant growth, with an area expansion of 9.42%. (3) The ecological restoration and management measures taken in the Yellow River Delta have proven notably effective. The proportion of Spartina alterniflora within the vegetation decreased by 3.45%, the native vegetation showed a resurgence, the distribution pattern of vegetation communities moved toward stability, and the total area of vegetation in the study area exhibited an upward trajectory.
Statistical Validation of a Physical Prime Random Number Generator Based on Quantum Noise
Authors: Ferreira Maurício J. , Silva Nuno A. , Pinto Armando N. , Muga Nelson J.
Published: November 2023

Random prime numbers are an essential resource for many asymmetric cryptographic protocols. However, despite the emerging popularity of quantum random number generators (QRNGs) as sources of secure randomness, physical prime number generators have not yet been explored. In this work, we experimentally implement and characterize a vacuum-based probabilistic prime number generation scheme with an error probability of 3.5×10−15. By removing the quantum source (QS), an additional scheme based on electronic noise is derived, and a comparative analysis for increasing prime lengths is made. We observed that the QS significantly outperforms the classical scheme for small prime generation, where increases up to 585.0% in the diversity of unique primes obtained are seen. Moreover, we propose a length-agnostic statistical test for prime number sequences and apply it to the output of the uniformized randomness source, which was successful in revealing underlying biases in the output prime distributions. The resultant sequences were subsequently submitted to the NIST statistical test suite, where the quantum and classical sources passed, respectively, 86.96% and 45.34% of the total test set applied.
A Decision Support System That Considers Risk and Site Specificity in the Assessment of Irrigation Water Quality (IrrigWQ)
Authors: du Plessis Heinrich M. , Annandale John G. , Benadé Nico
Published: November 2023

Irrigators are increasingly challenged to maintain or even increase production using less water, sometimes of poorer quality, and often from unconventional sources. This paper describes the main features of a newly developed software-based Decision Support System (DSS), with which the fitness for use (FFU) of water for irrigation (IrrigWQ) can be assessed. The assessment considers site-specific factors, several non-traditional water constituents, and the risk of negative effects. The water balance components of a cropping system and the redistribution of solutes within a soil profile are assessed with a simplified soil water balance and chemistry model. User-friendly, colour-coded output highlights the expected effects of water constituents on soil quality, crop yield and quality, and irrigation infrastructure. Because IrrigWQ uses mainly internationally accepted cause–effect relationships to assess the effect of water quality constituents, it is expected to find universal acceptance and application among users. IrrigWQ also caters for calculating so-called Water Quality Requirements (WQRs). WQRs indicate the threshold levels of water quality constituents for irrigation at specified levels of acceptability or risk. WQRs assist water resource managers in setting site-specific maximum threshold levels of water quality constituents that can be tolerated in a water source before impacting negatively on successful irrigation.
Spatiotemporal Characteristics of Hourly-Scale Extreme Precipitation in the Sichuan Basin and Its Impact on Normalized Difference Vegetation Index Values
Authors: Xiang Ying , Li Zhongliang , Wu Yixiao , Wang Keqing , Yang Jie
Published: November 2023

This study harnesses ground observation data collected between 1980 and 2021 and ERA5 hourly data to thoroughly implement trend and correlation analysis techniques to explore the spatiotemporal dynamic characteristics of daily and hourly extreme precipitation in the Sichuan Basin. The investigation delineates these characteristics and probes into the potential triggers of extreme hourly rainstorms. The findings unveil the following: (1) A general increase in extreme rainfall volume, contribution rate, intensity, and dispersion, along with a decline in frequency and proportion of rainstorm areas, indicating the concentration of daily-scale severe rainstorms. The basin’s edge receives more precipitation than the bottom, exhibiting latitudinal variations. (2) The northernmost mountainous regions have less frequent, less intense rainstorms influenced by terrain, whereas the northeastern region experiences more frequent, dispersed rainstorms. (3) Extreme hourly rainstorms predominantly occur at night, with rainfall amount, intensity, and frequency declining at 21:00 compared to 19:00. (4) Summer experiences the highest risk of extreme rainstorms, with annual and monthly datasets displaying a rising trend in the frequency, dispersion, and intensity of intense hourly rainstorms. (5) Peak values of extreme hourly rainstorms are growing, with two distinct periods for their frequency: 1:00–9:00 and 10:00–24:00, with an increase in the former and a decrease in the latter. (6) Normalized difference vegetation index (NDVI) values ascend from southwest to northeast within the basin on a ten-day scale, correlating with the distribution of hourly extreme precipitation.
Evaluation of an Alternative Functional Form to Fit the Lorenz Curve for the Concentration Index Calculation
Authors: Núñez-González Gerardo , Velázquez-Pérez Domingo , Pelayo-Cortés Francisco Javier
Published: November 2023

Precipitation concentration indices have become a popular tool for analyzing the structure of daily precipitation amounts. Among the existing indices, the concentration index (CI) is widely used. In calculating the CI, an important aspect is adjusting the Lorenz curve based on the observed precipitation data. Usually, the fit has been carried out with equations of the type y = axebx. However, in some research work, it has been observed that sometimes, the fit obtained only partially describes the behavior of the data. Thus, this work evaluated an alternative functional form to fit the Lorenz curve. For this, daily precipitation data from 44 climatological stations in Mexico were used to assess two equations for adjusting the Lorenz curve. Once the fit was made, the goodness of fit was evaluated to determine which of the functional forms best described the behavior of the data. Results showed that the two functional forms produced similar results for low precipitation concentrations. However, when the concentration increased, the alternative functional form generated results following the behavior of the observations. Thus, it is recommended to use the alternative functional form to avoid overestimations of the concentration of daily precipitation in areas where it is known that a high concentration occurs.
Coefficient Bounds for Some Families of Bi-Univalent Functions with Missing Coefficients
Authors: Analouei Adegani Ebrahim , Jafari Mostafa , Bulboacă Teodor , Zaprawa Paweł
Published: November 2023

A branch of complex analysis with a rich history is geometric function theory, which first appeared in the early 20th century. The function theory deals with a variety of analytical tools to study the geometric features of complex-valued functions. The main purpose of this paper is to estimate more accurate bounds for the coefficient |an| of the functions that belong to a class of bi-univalent functions with missing coefficients that are defined by using the subordination. The significance of our present results consists of improvements to some previous results concerning different recent subclasses of bi-univalent functions, and the aim of this paper is to improve the results of previous outcomes. In addition, important examples of some classes of such functions are provided, which can help to understand the issues related to these functions.
Analysis of Correlation Bounds for Uniformly Expanding Maps on [0, 1]
Authors: Abdelkader Mohamed
Published: November 2023

In this paper, we provide the decay of correlations for random dynamical systems. Precisely, we consider the uniformly C2 piecewise expanding maps defined on the unit interval satisfying λ(Tω′)=inf|Tω′|>2. As a principal tool of these studies, we use a coupling method for analyzing the coupling time of observables with bounded variation.
Effects of Neonicotinoid Pesticide Metabolic Compounds on Medaka (Oryzias latipes) Embryo Development
Authors: Kai Hotaka , Mita Arisa , Yamaguchi Masahiro
Published: November 2023

Neonicotinoids, including imidacloprid, are pesticides that resemble nicotine and undergo slight chemical alterations through metabolic changes in the environment. However, the effects of these metabolites on organisms remain unknown. In this study, we assessed the developmental processes of medaka embryos exposed to neonicotinoid metabolites. The target compounds were imidacloprid metabolites: 2-chloro-5-pyridine carbaldehyde (CPC) and 6-chloronicotinic acid (6-CNA). Medaka embryos within 6 h of fertilization were exposed to the compounds, and their developmental processes were observed under a stereomicroscope. Medaka embryos exposed to 5 mg/L CPC showed no abnormalities compared to the controls. Contrastingly, medaka embryos exposed to 10, 15, and 20 mg/L CPC showed abnormalities such as thrombus formation, asymmetry, disorganized development of the eyeballs, and low blood flow. This trend was more pronounced at higher CPC concentrations. On the other hand, embryos exposed to 80 and 160 mg/L 6-CNA showed no abnormalities until day 7 of exposure. However, on day 8 of exposure, sudden embryo death was observed. Both compounds may have bound to acetylcholine receptors as agonists; however, their effects were different. CPC caused abnormal development and 6-CNA caused inhibition of hatching gland development and/or synthesis of the hatching enzyme.
Adipokines and Bacterial Metabolites: A Pivotal Molecular Bridge Linking Obesity and Gut Microbiota Dysbiosis to Target
Authors: Turpin Teva , Thouvenot Katy , Gonthier Marie-Paule
Published: November 2023

Adipokines are essential mediators produced by adipose tissue and exert multiple biological functions. In particular, adiponectin, leptin, resistin, IL-6, MCP-1 and PAI-1 play specific roles in the crosstalk between adipose tissue and other organs involved in metabolic, immune and vascular health. During obesity, adipokine imbalance occurs and leads to a low-grade pro-inflammatory status, promoting insulin resistance-related diabetes and its vascular complications. A causal link between obesity and gut microbiota dysbiosis has been demonstrated. The deregulation of gut bacteria communities characterizing this dysbiosis influences the synthesis of bacterial substances including lipopolysaccharides and specific metabolites, generated via the degradation of dietary components, such as short-chain fatty acids, trimethylamine metabolized into trimethylamine-oxide in the liver and indole derivatives. Emerging evidence suggests that these bacterial metabolites modulate signaling pathways involved in adipokine production and action. This review summarizes the current knowledge about the molecular links between gut bacteria-derived metabolites and adipokine imbalance in obesity, and emphasizes their roles in key pathological mechanisms related to oxidative stress, inflammation, insulin resistance and vascular disorder. Given this interaction between adipokines and bacterial metabolites, the review highlights their relevance (i) as complementary clinical biomarkers to better explore the metabolic, inflammatory and vascular complications during obesity and gut microbiota dysbiosis, and (ii) as targets for new antioxidant, anti-inflammatory and prebiotic triple action strategies.
The Structural–Functional Crosstalk of the Calsequestrin System: Insights and Pathological Implications
Authors: Marabelli Chiara , Santiago Demetrio J. , Priori Silvia G.
Published: November 2023

Calsequestrin (CASQ) is a key intra-sarcoplasmic reticulum Ca2+-handling protein that plays a pivotal role in the contraction of cardiac and skeletal muscles. Its Ca2+-dependent polymerization dynamics shape the translation of electric excitation signals to the Ca2+-induced contraction of the actin-myosin architecture. Mutations in CASQ are linked to life-threatening pathological conditions, including tubular aggregate myopathy, malignant hyperthermia, and Catecholaminergic Polymorphic Ventricular Tachycardia (CPVT). The variability in the penetrance of these phenotypes and the lack of a clear understanding of the disease mechanisms associated with CASQ mutations pose a major challenge to the development of effective therapeutic strategies. In vitro studies have mainly focused on the polymerization and Ca2+-buffering properties of CASQ but have provided little insight into the complex interplay of structural and functional changes that underlie disease. In this review, the biochemical and structural natures of CASQ are explored in-depth, while emphasizing their direct and indirect consequences for muscle Ca2+ physiology. We propose a novel functional classification of CASQ pathological missense mutations based on the structural stability of the monomer, dimer, or linear polymer conformation. We also highlight emerging similarities between polymeric CASQ and polyelectrolyte systems, emphasizing the potential for the use of this paradigm to guide further research.
Efficacy of HDAC Inhibitors in Driving Peroxisomal β-Oxidation and Immune Responses in Human Macrophages: Implications for Neuroinflammatory Disorders
Authors: Villoria-González Andrea , Zierfuss Bettina , Parzer Patricia , Heuböck Elisabeth , Zujovic Violetta , Waidhofer-Söllner Petra , Ponleitner Markus , Rommer Paulus , Göpfert Jens , Forss-Petter Sonja , Berger Johannes , Weinhofer Isabelle
Published: November 2023

Elevated levels of saturated very long-chain fatty acids (VLCFAs) in cell membranes and secreted lipoparticles have been associated with neurotoxicity and, therefore, require tight regulation. Excessive VLCFAs are imported into peroxisomes for degradation by β-oxidation. Impaired VLCFA catabolism due to primary or secondary peroxisomal alterations is featured in neurodegenerative and neuroinflammatory disorders such as X-linked adrenoleukodystrophy and multiple sclerosis (MS). Here, we identified that healthy human macrophages upregulate the peroxisomal genes involved in β-oxidation during myelin phagocytosis and pro-inflammatory activation, and that this response is impaired in peripheral macrophages and phagocytes in brain white matter lesions in MS patients. The pharmacological targeting of VLCFA metabolism and peroxisomes in innate immune cells could be favorable in the context of neuroinflammation and neurodegeneration. We previously identified the epigenetic histone deacetylase (HDAC) inhibitors entinostat and vorinostat to enhance VLCFA degradation and pro-regenerative macrophage polarization. However, adverse side effects currently limit their use in chronic neuroinflammation. Here, we focused on tefinostat, a monocyte/macrophage-selective HDAC inhibitor that has shown reduced toxicity in clinical trials. By using a gene expression analysis, peroxisomal β-oxidation assay, and live imaging of primary human macrophages, we assessed the efficacy of tefinostat in modulating VLCFA metabolism, phagocytosis, chemotaxis, and immune function. Our results revealed the significant stimulation of VLCFA degradation with the upregulation of genes involved in peroxisomal β-oxidation and interference with immune cell recruitment; however, tefinostat was less potent than the class I HDAC-selective inhibitor entinostat in promoting a regenerative macrophage phenotype. Further research is needed to fully explore the potential of class I HDAC inhibition and downstream targets in the context of neuroinflammation.
Dissociation of Implicit and Explicit Interpretation Bias: The Role of Depressive Symptoms and Negative Cognitive Schemata
Authors: Wessa Michèle , Domke-Wolf Mila , Jungmann Stefanie M.
Published: November 2023

A negative interpretation bias appears to depend on several depression-related state and trait characteristics, most notably depressive symptoms, negative mood, and negative cognitive schemas. While empirical findings for explicitly assessed interpretation bias are rather consistent, implicit measures have revealed heterogeneous results. In this context, we present two studies investigating the relationship between implicit and explicit interpretation bias and depression- and anxiety-related state and trait variables. In the first study, we conducted an implicit ambiguous cue-conditioning task (ACCT) with 113 young, healthy individuals. In the second study, we utilized an explicit ambiguous social situations task (DUCTUS) with 113 young, healthy individuals. Additionally, a subsample of 46 participants completed both the ACCT and DUCTUS tasks to directly relate the two bias scores obtained from the implicit and explicit assessment methods, respectively. In the first study, regression analysis revealed no significant predictors for the implicit interpretation bias. However, in the second study, the explicit negative interpretation bias was significantly predicted by female gender, depressive symptoms, and dysfunctional cognitive schemas. For the subsample that completed both tasks, we observed no significant correlation between the two bias scores obtained from the ACCT and DUCTUS. These results suggest that implicit and explicit interpretation biases are differently associated with depression-related trait and state characteristics, indicating that they represent different aspects of biased information processing.
Field Study to Compare and Evaluate Summer Thermal Comfort of School Buildings with Different Moderate Thermal Mass in Their Building Elements
Authors: Su Bin , McPherson Peter , Jadresin Milic Renata , Wang Xinxin , Shamout Sameh , Liang Yifeng
Published: November 2023

Previous studies show that moderate thermal mass in school building elements can positively impact the winter indoor thermal environment in a temperate climate with mild, humid winters. Based on a field study, this research contributes new physical data of the summer indoor thermal environment of Auckland school buildings with different designs of moderate thermal mass in their building elements to add to the previous winter field-study data and demonstrates that a school building with moderate thermal mass is adequate in a temperate climate with mild, humid winters and warm, dry summers. This field study compared and evaluated the summer indoor thermal environment of classrooms with different moderate thermal mass in their building elements during the summer school term and the summer school holidays. This study found that a classroom with thermal mass in its building elements has 19% to 21% more time in summer than a classroom without any thermal mass in its building elements when indoor air temperatures are within the thermal comfort zone, which was solely impacted by the building’s thermal performance. This study established a suitable research method to analyse the field-study data and identify the differences in the indoor thermal environments of the school buildings with different designs of moderate thermal mass in their building elements.
Architectural Evaluation of Healthcare Facilities: A Comprehensive Review and Implications for Building Design
Authors: Jaušovec Marko , Gabrovec Branko
Published: November 2023

The architectural design of healthcare institutions impacts patient experience and health outcomes. Facing challenges like demographic aging and evolving treatments, this study reviewed fourteen key publications on healthcare architecture. The insights gained have highlighted the need for qualitative methods to understand these environments. An integrated healthcare architecture framework (IHAF) was developed, integrating architectural guidelines, user experience, and sustainability. The IHAF emphasizes inclusive design, adaptability, patient-centricity, and staff well-being, focusing on eco-friendly practices and green spaces in healthcare facilities. Advocating a multidisciplinary design approach, the IHAF aims to create functional, sustainable, patient-focused environments. This research identifies the need for qualitative methods unique to the Slovenian healthcare context. It aims to inform architectural solutions for healthcare institutions, highlighting the role of design in optimal patient care and well-being.
Digital Prefabrication of Lightweight Building Elements for Circular Economy: Material-Minimised Ribbed Floor Slabs Made of Extruded Carbon Reinforced Concrete (ExCRC)
Authors: Bosbach Sven , Kalthoff Matthias , Morales Cruz Cynthia , Adam Viviane , Matschei Thomas , Classen Martin
Published: November 2023

To reduce resource consumption and the carbon footprint of the construction industry, new construction principles that stipulate the minimisation of materials are urgently needed. Floor slabs show high potential for saving materials and CO2 emissions, as they account for around 50% of the total volume of concrete used worldwide. A promising approach is the use of corrosion-resistant carbon fibre-reinforced polymer (CFRP) reinforcement, which requires only a small concrete cover to meet bond requirements. Regarding the component’s topology, material-efficient ribbed or waffle slabs have been built for decades but their execution has declined owing to their labour-intense production, which calls for manual installation of the formwork. A recently proposed extrusion manufacturing process combines both approaches for material-minimised construction and allows the fast and formwork-free production of carbon reinforced concrete (CRC) components. This article describes the concept, the finding of the form, fabrication and experimental testing of an innovative ribbed floor slab composed of precast extruded carbon reinforced concrete (ExCRC) webs. The novel floor slab consists of a conventionally cast thin-walled top slab and shaped, load-adapted ExCRC webs to achieve high utilisaton of the structural member. The ribbed slab provides a lightweight structural system with drastic savings in dead load compared with a conventional steel reinforced slab. In addition, the high durability and service life of the novel floor slabs makes them ideal components to be recovered after an initial life cycle and to be re-used in new construction projects. The paper gives an outlook to the full-scale production of one-way and two-way slab systems made of ExCRC such as ribbed slabs, waffle slabs and sandwich slabs with a honeycomb infill.
Exploring the Promise of Second-Line Chemotherapy in Biliary Tract Tumours: A Glimpse into Novel Treatment Approaches
Authors: Villalba Cuesta Paula , Avedillo Ruidiaz Mercedes , Ruiz Hispán Eva , Fuentes Mateos Raquel , Lamarca Angela
Published: November 2023

Biliary tract tumours, including bile duct, gallbladder, and ampulla of Vater malignancies, pose a rare but formidable oncologic challenge. Typically diagnosed at advanced stages, these tumours offer limited treatment options and dismal prognoses, with a five-year survival rate below 20%. First-line chemotherapy with gemcitabine-cisplatin has demonstrated only modest efficacy, leaving a pressing need for improved therapeutic strategies. This comprehensive review provides a detailed examination of the current landscape of second-line chemotherapy for biliary tract tumours. The pivotal ABC-06 trial established FOLFOX (5-fluorouracil, leucovorin, and oxaliplatin) as the standard second-line therapy, demonstrating improved overall survival compared to active symptom control alone. Conversely, the NIFTY trial introduced nal-IRI (nanoliposomal irinotecan) plus 5-FU/LV (5-fluorouracil and leucovorin) as an alternative option, demonstrating substantial gains in progression-free and overall survival. However, the posterior NALIRICC trial presented conflicting results, raising questions about the added benefit of nal-IRI. Challenges in delivering second-line chemotherapy include rapid patient performance deterioration post-first-line treatment and limited access to second-line therapy. Only a fraction of eligible patients receive second-line therapy, emphasising the need for more effective first-line therapies to maintain patient fitness. The role of monotherapy in the second-line setting remains uncertain, particularly in unfit patients, and the absence of biomarkers for tailored treatment underscores the need for ongoing research. While challenges persist, ongoing investigations offer hope for optimising second-line therapy for biliary tract tumours, promising improved outcomes for patients facing this disease. This review provides an overview of current facts and challenges when delivering second-line chemotherapy for advanced biliary tract tumours.
Supportive Care in Pediatric Oncology: Opportunities and Future Directions
Authors: Freedman Jason L. , Beeler Dori M. , Bowers Alison , Bradford Natalie , Cheung Yin Ting , Davies Maya , Dupuis L. Lee , Elgarten Caitlin W. , Jones Torri M. , Jubelirer Tracey , Miller Tamara P. , Patel Priya , Phillips Charles A. , Wardill Hannah R. , Orsey Andrea D.
Published: November 2023

The optimization of outcomes for pediatric cancer patients relies on the successful advancement of supportive care to ease the treatment burden and mitigate the long-term impacts of cancer therapy. Advancing pediatric supportive care requires research prioritization as well as the development and implementation of innovations. Like the prevailing theme throughout pediatric oncology, there is a clear need for personalized or precision approaches that are consistent, evidence-based, and guided by clinical practice guidelines. By incorporating technology and datasets, we can address questions which may not be feasible to explore in clinical trials. Now is the time to listen to patients’ voices by using patient-reported outcomes (PROs) to ensure that their contributions and experiences inform clinical care plans. Furthermore, while the extrapolation of knowledge and approaches from adult populations may suffice in the absence of pediatric-specific evidence, there is a critical need to specifically understand and implement elements of general and developmental pediatrics like growth, nutrition, development, and physical activity into care. Increased research funding for pediatric supportive care is critical to address resource availability, equity, and disparities across the globe. Our patients deserve to enjoy healthy, productive lives with optimized and enriched supportive care that spans the spectrum from diagnosis to survivorship.