bluuebunny/crossref_metadata_2025 huggingface dataset:
Science for transformative change: the IPCC, boundary work and the making of useable knowledge
Authors: Lidskog Rolf
Published: October 2024

While there has been much discussion about what kind of expertise the IPCC needs to develop to (better) guide climate policy, little has been said about how the experts themselves assess the challenges of making science policy-relevant. The paper aims to address this gap by exploring how leading IPCC experts reflect on and evaluate their work. The empirical material consists of an interview study with experts currently or recently involved in the IPCC. The selection strategy aimed to achieve a broad range of experience among those with key roles in the assessment work, including experts from all three working groups, from different regions, and of different genders. Data from the interviews was analyzed thematically using NVivo. The concept of boundary work was used to analyze the distinctions and boundaries in this work; how the IPCC experts draw boundaries between science and policy, between policy-relevance and policy-prescriptiveness, and between certain and uncertain knowledge. By analyzing the experts’ own experiences and ideas about what makes science relevant to policy-making, the paper contributes to the discussion about current and future challenges for the IPCC.
Predictive value of geriatric nutritional risk index in cardiac and cerebrovascular events after endovascular aortic aneurysm repair
Authors: Zou YuPei , Wang Jiarong , Zhao Jichun , Ma Yukui , Huang Bin , Yuan Ding , Liu Yang , Han Maonan , Gan Huatian , Yang Yi
Published: October 2024

Objective

To evaluate the effect of malnutrition assessed by the Geriatric Nutritional Risk Index (GNRI) on major adverse cardiac and cerebrovascular events (MACCE) in the elderly patients after endovascular aortic aneurysm repair (EVAR).

Materials and methods

This was a retrospective cohort study of elderly patients who underwent EVAR in a tertiary hospital. Malnutrition status was assessed by the GNRI. The primary outcome was MACCE. The predictive ability of the GNRI was compared with both the Revised Cardiac Risk Index (RCRI) and the modified Frailty Index (mFI) using Receiver operating characteristic (ROC) curve.

Result

A total of 453 patients underwent EVAR November 2015 and January 2020 was retrospectively analyzed, equally divided into three (low/medium/high) groups according to GNRI values which ranked from low to high. Five (1.10%) patients were lost in follow-up after surgery, and the median length of follow-up was 28.00 (15.00–47.00) months. The high GNRI values reduced length of hospital stay following EVAR in comparison to patients in low GNRI values group (β 9.67, 95% CI 4.01–23.32, p = 0.0113; adjusted β −1.96, 95% CI −3.88, −0.05, p = 0.0454). GNRI status was associated with a significantly increased risk of long-term mortality after EVAR (Medium GNRI, unadjusted HR 0.40, 95%CI 0.23–0.70, p = 0.0014; adjusted HR 0.47, 95%CI 0.26–0.84, p = 0.0107; high GNRI, 0.27 95%CI 0.14–0.55; p = 0.0003; adjusted HR 0.32 95%CI 0.15–0.68, p = 0.0029). Both medium and high GNRI values were linked to significantly reduced risks of MACCE compared to low GNRI score patients (Medium GNRI, unadjusted HR 0.34, 95%CI 0.13–0.88, p = 0.00265; adjusted HR 0.37, 95%CI 0.14–0.96, p = 0.0408; High GNRI, 0.26 95%CI 0.09–0.78; p = 0.0168; adjusted HR 0.21 95%CI 0.06–0.73, p = 0.0029). Compared with the RCRI and mFI, the GNRI had better discrimination in predicting long-term MACCE. An area under the curve (AUC) for GNRI mFI, and RCRI is 0.707, 0.614 and 0.588, respectively. (Z statistic, GNRI vs. mFI, p = 0.0475; GNRI vs. RCRI, p = 0.0017).

Conclusion

Malnutrition assessed by the GNRI may serve as a useful predictor of long-term MACCE in elderly patients after EVAR, with preferable discrimination abilities compared with both RCRI and mFI.
RCLNet: an effective anomaly-based intrusion detection for securing the IoMT system
Authors: Shaikh Jamshed Ali , Wang Chengliang , Muhammad Wajeeh Us Sima , Arshad Muhammad , Owais Muhammad , Alnashwan Rana Othman , Chelloug Samia Allaoua , Muthanna Mohammed Saleh Ali
Published: October 2024

The Internet of Medical Things (IoMT) has revolutionized healthcare with remote patient monitoring and real-time diagnosis, but securing patient data remains a critical challenge due to sophisticated cyber threats and the sensitivity of medical information. Traditional machine learning methods struggle to capture the complex patterns in IoMT data, and conventional intrusion detection systems often fail to identify unknown attacks, leading to high false positive rates and compromised patient data security. To address these issues, we propose RCLNet, an effective Anomaly-based Intrusion Detection System (A-IDS) for IoMT. RCLNet employs a multi-faceted approach, including Random Forest (RF) for feature selection, the integration of Convolutional Neural Networks (CNN) and Long Short-Term Memory (LSTM) models to enhance pattern recognition, and a Self-Adaptive Attention Layer Mechanism (SAALM) designed specifically for the unique challenges of IoMT. Additionally, RCLNet utilizes focal loss (FL) to manage imbalanced data distributions, a common challenge in IoMT datasets. Evaluation using the WUSTL-EHMS-2020 healthcare dataset demonstrates that RCLNet outperforms recent state-of-the-art methods, achieving a remarkable accuracy of 99.78%, highlighting its potential to significantly improve the security and confidentiality of patient data in IoMT healthcare systems.
Variable preservation of the 1991 Hudson tephra in small lakes and on land
Authors: Streeter Richard T. , Cutler Nick A. , Lawson Ian T. , Hutchison William , Dominguez Lucia , Hiles William
Published: October 2024

Volcanic ash (tephra) preserved in terrestrial environments and lake sediments contains information about volcanic processes and can be used to infer eruptive parameters and frequency of past eruptions, contributing to the understanding of volcanic hazards. However, tephra deposits can undergo transformation from their initial fallout sedimentation to being preserved as a tephra layer in the sedimentary record. The process is likely to be different in lakes and in terrestrial (soil) sequences. Here we compare the thickness, mass loading and grain size of tephra layers from the 1991 eruption of Cerro Hudson, Chile, from small lakes and adjacent terrestrial settings to measurements of the tephra made shortly after the eruption. We analysed samples from 35 cores in total from six small lakes (<0.25 km2), located 76 and 109 km from the volcano in two contrasting climatic areas (cool and humid northern site, and warm and dry southern site), and made 73 measurements of tephra thickness and 11 measurements of grain size in adjacent terrestrial areas. The major element geochemistry of our samples confirmed they were from the 1991 Hudson eruption. We found that some of the measured characteristics of the preserved tephra layers were comparable to those recorded in 1991 shortly after initial deposition, but that there was considerable variability within and between locations. This variability was not predictable and lake sediments did not preserve a notably more accurate record of the fallout than terrestrial sites. However, in aggregate the characteristics of the preserved tephra was similar to those recorded at the time of deposition, suggesting that, for palaeotephra research, a sampling strategy involving a wide range of environments is more robust than one that relies on a single sedimentary record or a single type of sedimentary environment.
Affordances of community-oriented praxis in teacher education: a configurative review
Authors: Kitooke Amoni , Mahon Kathleen
Published: October 2024

This configurative review explores how nurturing and enacting a community-oriented praxis (COP) in teacher education can enhance teacher candidates’ preparedness for teaching roles whilst addressing some broader educational and societal concerns. Here, CoP is defined as practices that consider, connect with, and draw on local community strengths, needs, and aspirations for the good of individuals, communities, and society more broadly. We review 31 cases in 37 articles on explicitly community-oriented teacher education (COTE) initiatives from nine country contexts where teacher educators, school-based mentors, and members of local communities collaboratively facilitate the learning experiences of participants in teacher education (i.e., teacher candidates). An analysis of the motivations, processes, and outcomes of the initiatives in the COTE cases highlights benefits such as developing teacher candidates’ pedagogical content knowledge and research competence; fostering social justice consciousness and critical action; and facilitating the sustainable recruitment and retention of teachers especially in marginalised contexts. Implications and possible directions for teacher education are discussed.
Dairy consumption and its association with anthropometric measurements, blood glucose status, insulin levels, and testosterone levels in women with polycystic ovary syndrome: a comprehensive systematic review and meta-analysis
Authors: Rastad Hadith , Shahrestanaki Ehsan , Heydarian Hamid Reza , Maarefvand Mina
Published: October 2024

Background

We conducted a systematic review and meta-analysis on dairy consumption and its association with anthropometric measurements, blood glucose status, insulin levels, and testosterone levels in women with Polycystic Ovary Syndrome.

Methods

This study conducted a comprehensive literature search using electronic databases like MEDLINE, Scopus, PubMed, Web of Science, and Google Scholar to identify observational and interventional studies investigating the relationship between dairy product consumption and Polycystic Ovary Syndrome. A meta-analysis was performed on clinical trial studies that examined the effect of a low starch/low dairy diet in Polycystic Ovary Syndrome subjects. Statistical analyses were performed using Stata version 16.0 (Stata Corporation, College Station, Texas, USA), and statistical significance was defined as p-value < 0.05.

Results

Of the 1,313 citations reviewed, our systematic review identified 11 studies that met the inclusion criteria, comprising six case-control studies, four clinical trials, and one cross-sectional study. The case-control studies found limited evidence of an association between dairy consumption and Polycystic Ovary Syndrome. The result of the clinical trial studies in meta-analysis showed that reducing dairy intake along with reducing starch intake led to statistically significant improvements in anthropometric and metabolic measures including mean weight (Standardized mean difference: -8.43 (95% CI: -9.01, -7.86)), Body mass index (-3.14 (95% CI: -3.35, -2.92), waist circumference (-6.63 (95% CI: -10.70, -2.57)) and Waist-to-Height Ratio (-0.04 (95% CI: -0.07, -0.01), insulin fasting (-18.23 (95% CI: -22.11, -14.36)), insulin 120 minutes (-94.05 (95% CI: -157.67, -30.42)), HbA1c (-0.27 (95% CI: -0.37, -0.17)), Ferryman-Gallwey score (-2.07 (95% CI: -2.98, -1.16)) and total testosterone (-9.97 (95% CI: -14.75, -5.19)). No significant reduction was found in fasting glucose, 2 hours glucose, percent of fat mass, and mean free testosterone after intervention.

Conclusions

The findings of this systematic review show limited evidence about the association between dairy consumption and Polycystic Ovary Syndrome. The interventional studies suggest that a low-dairy/low-starch diet may improve some anthropometric and metabolic measures in women with Polycystic Ovary Syndrome.
Deep learning with ensemble approach for early pile fire detection using aerial images
Authors: Joshi Dhyey Divyeshkumar , Kumar Satish , Patil Shruti , Kamat Pooja , Kolhar Shrikrishna , Kotecha Ketan
Published: October 2024

Wildfires rank among the world’s most devastating and expensive natural disasters, destroying vast forest resources and endangering lives. Traditional firefighting methods, reliant on ground crew inspections, have notable limitations and pose significant risks to firefighters. Consequently, drone-based aerial imaging technologies have emerged as a highly sought-after solution for combating wildfires. Recently, there has been growing research interest in autonomous wildfire detection using drone-captured images and deep-learning algorithms. This paper introduces a novel deep-learning-based method, distinct in its integration of infrared thermal, white, and night vision imaging to enhance early pile fire detection, thereby addressing the limitations of existing methods. The study evaluates the performance of machine learning algorithms such as random forest (RF) and support vector machines (SVM), alongside pre-trained deep learning models including AlexNet, Inception ResNetV2, InceptionV3, VGG16, and ResNet50V2 on thermal-hot, green-hot, and white-green-hot color images. The proposed approach, particularly the ensemble of ResNet50V2 and InceptionV3 models, achieved over 97% accuracy and over 99% precision in early pile fire detection on the FLAME dataset. Among the tested models, ResNet50V2 excelled with the thermal-fusion palette, InceptionV3 with the white-hot and green-hot fusion palettes, and VGG16 with a voting classifier on the normal spectrum palette dataset. Future work aims to enhance the detection and localization of pile fires to aid firefighters in rescue operations.
IVIG response in pediatric acute-onset neuropsychiatric syndrome correlates with reduction in pro-inflammatory monocytes and neuropsychiatric measures
Authors: Melamed Isaac , Rahman Shamma , Pein Heather , Heffron Melinda , Frankovich Jennifer , Kreuwel Huub , Mellins Elizabeth D.
Published: October 2024

Introduction

Pediatric Acute-Onset Neuropsychiatric Syndrome (PANS) is characterized by abrupt onset of obsessive-compulsive disorder or eating restriction along with the abrupt onset of other co-occurring symptoms (tics, behavioral and cognitive regression, etc.). PANS is thought to be a post-infectious immunopsychiatric disorder, although as with most post-infectious disorders, it is challenging to establish a causal relationship with proposed infectious triggers. Intravenous immunoglobulin (IVIG) can modulate inflammation and support the elimination of infection and has been used for treatment of many post-infectious inflammatory disorders and autoimmune conditions. The aim of the study is to explore the pro-inflammatory state in PANS before and after administration of IVIG.

Methods

Children with moderate-to-severe PANS received six infusions of IVIG (Octagam 5%, Octapharma) every 3 weeks with post treatment follow-up. Blood samples and psychiatric measures were obtained at Visits 1 (pre-treatment), 7 and 8 (4 and 11 weeks after last infusion, respectively). Myeloid cell activation was assessed via flow cytometry.

Results

All ten patients included in the study were male, White, with mean age 12.4 years (range 6–16). Statistically significant improvements following IVIG treatment were demonstrated in all psychometric assessments and parent questionnaires including CY-BOCS (obsessive compulsive scale), YGTSS (tic scale) and a parent PANS rating scale (for all scales p<0.001). The fraction of pro-inflammatory monocytes and dendritic cells decreased from pre-IVIG treatment levels. The proportional reductions were not compensated by increases in total white blood cells; pro-inflammatory monocytes post-IVIG were decreased as a proportion of CD14+ myeloid cells and in absolute number.

Conclusions

The results of this study suggest that active PANS is associated with a pro-inflammatory state. This pro-inflammatory profile and psychometric scores improved following IVIG treatment. Future work will aim to further elucidate the roles of innate and adaptive immune responses in PANS and the regulatory mechanism(s) of IVIG in PANS treatment.
Effect of methimazole treatment on Th1, Th17, and Th22 lymphocytes in pediatric Graves’ disease patients
Authors: Starosz Aleksandra , Stożek Karolina , Opęchowska Aleksandra , Bossowski Filip , Moniuszko Marcin , Grubczak Kamil , Bossowski Artur
Published: October 2024

Graves’ disease is the leading cause of autoimmune hyperthyroidism. Thyroid hormones are an essential element of the endocrine system, playing a pivotal role in the body’s development, especially important in children with intensified growth. Disturbance within thyroid tissue certainly affected the whole body. Nowadays, numerous research studies indicate different factors contributing to the onset of the disease; however, the exact pathomechanism of Graves’ disease is still not fully understood, especially in the context of immune-related processes. Th1, Th17, and Th22 effector lymphocytes were found to be crucial participants in the disease outcome, as well as in autoimmune diseases. Here, our study aimed at assessing selected effector T lymphocytes, Th1, Th17, and Th22, in newly diagnosed pediatric Graves’ disease patients, together with their association with thyroid-related parameters and the potential outcome of disease management. We indicated significant increases in the frequencies and absolute numbers of selected effector lymphocytes in Graves’ disease patients. In addition, their mutual ratios, as well as Th1/Th17, Th/Th22, and Th17/Th22, seem to be significant in those diseases. Notably, low Th17/Th22 ratio values were distinguished as potential prognostic factors for normalizing TSH levels in response to methimazole treatment. To sum up, our research determines the crucial contribution of Th1, Th17, and Th22 cells in the pathogenesis of Graves’ disease. Moreover, the mentioned subset of T cells is highly likely to play a substantial role in the potential prediction of therapy outcomes.
Exploring pathogen population density as a metric for understanding post-COVID infectious disease surges
Authors: Zhang Luwen
Published: October 2024

After the easing of COVID-19 restrictions, peaks of common infectious diseases surpassed pre-pandemic levels, raising questions about causes and ways to monitor these changes. A proposed measure, the Pathogen Population Density (PPD) score, could help track these shifts. PPD refers to the concentration of infectious agents within a population at a given time and location, serving as a potential indicator of infection levels in susceptible individuals at the population level. It is likely that PPD remains relatively stable within a specific community, as an equilibrium forms between infections and susceptibility. During the pandemic, nonpharmaceutical interventions (NPIs) led to a reduction in infectious diseases, possibly lowering population immunity and decreasing the PPD score. Once NPIs were lifted, the PPD score likely increased sharply due to a larger pool of susceptible individuals, causing more primary infections and stronger recurrent infections, faster transmission, and more severe pathogenic outcomes at the individual level. Monitoring the PPD score over time could help predict when infection peaks will occur. PPD is influenced by factors such as public health strategies, vaccination programs, and the behavior of high-risk individuals. As a quantitative measure, PPD has the potential to serve as a valuable predictive and monitoring tool, helping public health officials anticipate and track changes in infectious disease dynamics. It could be an effective tool for managing future outbreaks or pandemics and serve as a communication tool between scientists and the public to understand the emergence of new disease peaks.
Investigating concentrations and sources of polycyclic aromatic hydrocarbons in South and Central Texas bays and estuaries along the Gulf of Mexico, USA
Authors: Lloyd Jack , Lu Kaijun , Liu Zhanfei
Published: October 2024

Polycyclic aromatic hydrocarbons (PAHs) are among the most widespread organic contaminants in the environment, and anthropogenic activities can produce PAHs through a variety of pyrogenic or petrogenic means. Knowing the concentrations and sources of PAHs helps evaluate ecosystem health and manage natural resources. In this study, 16 US Environmental Protection Agency priority PAHs were analyzed in water and sediment samples collected from September 2021 to September 2023 in four bay systems along the south and central Texas coast, which are a hotpot of crude oil transportation in the United States. Our results indicated that the total concentration of PAHs ranged from 1.9 to 8.3 ng/mL in surface waters (< 0.5 m) and from 520 to 1257 ng/g in surface sediments (top 5 cm). Grain size analysis revealed that the sediment was dominated by silt (4 - 63 μm), followed by clay (< 4 μm) and sand (> 63 μm) fractions. Both organic carbon and clay content were shown to play a significant role in controlling the PAH content in sediments. Diagnostic ratios indicated that PAHs were primarily sourced via pyrolytic processes, such as the combustion of fossil fuels. Additional sampling at Port Bay, a shallow, secondary bay in the Mission-Aransas National Estuarine Research Reserve, implicated a strong role of resuspension in the distribution and composition of PAHs in the bay systems studied. Overall, these data offer insights into the concentration levels and sources of PAHs in this key region housing oil production and transportation in the United States.
Respiration rates and its relationship with ETS activity in euphausiids: implications for active flux estimations
Authors: Couret María , Díaz-Pérez Javier , Sarmiento-Lezcano Airam N. , Landeira José María , Hernández-León Santiago
Published: October 2024

Euphausiids, commonly known as krill, are crucial contributors to the ocean’s active carbon pump, impacting carbon export and sequestration through their diel vertical migration. These organisms feed on organic matter in the epipelagic layer at night and release inorganic carbon in the mesopelagic layer during the day via respiration. Measuring respiration in the mesopelagic layer is challenging due to the difficulties in obtaining direct measurements, as well as the lack of comprehensive data, and reliance on conservative estimates. The measurement of the electron transfer system (ETS) activity is used as a proxy to assess respiration in the mesopelagic layer. However, accurate calibration of respiration rates and ETS activity is imperative through experimental measurements and empirical data. Here, we compared the respiration rates with their respective ETS activities of different species of euphausiids captured at night in the epipelagic layer of the Atlantic Ocean along a latitudinal (42-29°N, 25°W) and a longitudinal (25-13°W, 29°N) transect. Our results revealed a spatial trend in respiration rates, and consequently in ETS activities, with rates decreasing southward and increasing slightly towards the African upwelling region. The Generalized Additive Model (GAM) demonstrated that epipelagic oxygen concentration, chlorophyll a, and the interaction between epipelagic temperature and mesopelagic oxygen concentration significantly influenced euphausiids respiration rates. Furthermore, we observed a strong correlation between respiration and specific ETS activities, with R/ETS ratios exceeding the conservative value of 0.5, which is typically used to estimate respiratory flux.
Integrative nursing interventions: knowledge, attitudes and practice in home nursing services in Germany—a quantitative and qualitative online survey
Authors: Stolz Regina , Klocke Carina , Mahler Cornelia , Valentini Jan , Joos Stefanie
Published: October 2024

Introduction

Integrative nursing interventions (INI) play a significant role in healthcare, particularly in the prevention and treatment of chronic diseases. Integrating evidence-based INI into healthcare aligns with global initiatives such as the WHO’s Decade of Healthy Aging 2020–2030. Many INI are low-threshold practices, empowering patients to independently manage health. However, the extent to which INI are used by home-care nursing-services (HNS) remains largely unknown. This study aims to explore the field of INI in German HNS regarding nurses’ use of INI as well as attitudes, subjective knowledge, and information needs on the subject.

Methods

A cross-sectional anonymous online survey with 29 Likert scale items and two open-ended questions was conducted between April 2023 and July 2023. The survey targeted nurse managers of HNS in Baden-Württemberg, Germany. Descriptive analysis was performed for quantitative data, while content analysis according to Kuckartz was applied to analyze open-ended text responses.

Results

In total, n = 68 out of n = 1,331 HNS took part in the survey yielding a response rate of 5.1%. Their overall attitude toward INI was clearly positive (10-point Likert scale M ± SD: 8.37 ± 2.22). The average self-assessed knowledge level about INI was moderate (M ± SD: 5.39 ± 2.76). Almost half of the participants (45.6%) declared to incorporate INI in patient care. Most participants (84.2%) lacked employees with additional qualifications in INI. The INI used most were medicinal herbal teas (61%), compresses (57%), and aromatherapy (48%). Acupressure showed the greatest disparity between actual use in participating HNS (4.3%) and interest in further education (61%). The most common symptoms for which INI are used are pain, respiratory problems, anxiety, and palliative care. The main challenges reported for the use of INI in HNS are financial aspects, qualification and limited resources (staff and time).

Discussion

This exploratory study provides the first insights into nurses’ attitudes, self-assessed knowledge, and utilization of INI in German HNS. Overall response rate was low (5.1%), therefore, the results should be interpreted with caution. Urgent action is needed to address financial aspects and further education on INI, to promote integration of INI in HNS to the best possible extent.
Exploring the therapeutic mechanisms of Yikang decoction in polycystic ovary syndrome: an integration of GEO datasets, network pharmacology, and molecular dynamics simulations
Authors: Miao Jiang , Gao LiXuan , Liu Xi , Cai Wenpin , Chen Lei , Chen Mojinzi , Sun Yun
Published: October 2024

Objective

The incidence of Polycystic Ovary Syndrome (PCOS) is increasing annually. This study aims to investigate the therapeutic mechanisms of Yikang Decoction (YKD) in the treatment of PCOS through the integration of GEO datasets, network pharmacology, and dynamic simulation.

Methods

Active ingredients of YKD and their targets were collected from the Traditional Chinese Medicine Systems Pharmacology (TCMSP) platform. Disease-relevant targets for PCOS were retrieved from several databases, including GeneCards, OMIM, PharmGKB, DrugBank, and GEO. The underlying pathways associated with the overlapping targets between YKD and PCOS were identified using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The mechanisms of interaction between the core targets and components were further explored through molecular docking and molecular dynamics simulations (MD).

Results

139 potential active components and 315 targets of YKD were identified. A topological analysis of the PPI network revealed 10 core targets. These targets primarily participated in the regulation of biological processes, including cell metabolism, apoptosis, and cell proliferation. The pathways associated with treating PCOS encompassed PI3K-Akt signaling pathway, Lipid and atherosclerosis, MAPK signaling pathways, and Endocrine resistance signaling pathways. Moreover, molecular docking and MD have been shown to reveal a good binding capacity between active compounds and screening targets.

Conclusion

This study systematically investigates the multi-target mechanisms of YKD in the treatment of PCOS, with preliminary verification provided through molecular docking and MD. The findings offer compelling evidence supporting the efficacy of YKD in treating PCOS.
The relationship between visual health and influencing factors among primary and secondary school students: a survey based in the Ningxia Hui Autonomous Region of China
Authors: Xu Jiyan , Sang Mengjiao , Xu Weiwei , Feng Kaijie
Published: October 2024

Introduction

The myopia rate of young people around the world, especially in China, has continued to rise, and the vision health of primary and secondary school students has gradually become a global concern. It was to explore the current characteristics of vision status and their intrinsic links to influencing factors of the Ningxia Hui Autonomous Region primary and secondary school students under regional characteristics.

Methods

1,670 primary and secondary school students in Ningxia Hui Autonomous Region were surveyed using Vision examination, International Physical Activity Scale, Lifestyle Behavior Scale. The data were analyzed using SPSS 25.0 and AMOS 23.0.

Results

The prevalence of myopia among primary and secondary school students in the Ningxia Hui Autonomous Region was 27.3%, with rates of 31.5% for girls and 23.4% for boys. There was a significant negative correlation between lifestyle behaviors (r = -0.36, p < 0.01) and physical activity level (r = –0.06, p < 0.05) with vision status. The physical activity level played a significant masking effect between lifestyle behaviors and vision status (a*b = 0.002, c’ = –0.044). Gender moderated the effects of lifestyle behaviors on physical activity level and vision status, as well as the effects of physical activity level on vision status. These factors constituted a mixed model with mediating and moderation. The model fitted well (RMSEA = 0.028, CFI = 0.951, NFI = 0.918).

Discussion

The prevalence of myopia among primary and secondary school students in this region is lower than the national average in China, and it is associated with lifestyle behaviors, physical activity level, and gender. When effectively controlled, the physical activity level significantly will reduce the prevalence of myopia as a masking variable. Gender, as a moderating variable, provides theoretical support for the classification and prevention of myopia. Regional surveys enriched the global database on adolescent myopia research, revealing the characteristics and common factors of visual problems in adolescents. Relevant departments and schools should integrate eye care into the national health security system, implement policies related to myopia prevention and control and safeguard the visual health of primary and secondary school students.
Development and validation of a web-based nomogram for acute kidney injury in acute non-variceal upper gastrointestinal bleeding patients
Authors: Wei Chaolian , Cao Honghua , Huang Lina , Feng Lu-Huai
Published: October 2024

Background

Acute kidney injury (AKI) is a common and serious complication in patients with acute non-variceal upper gastrointestinal bleeding (NVUGIB). Early prediction and intervention are crucial for improving patient outcomes.

Methods

Data for patients presenting with acute NVUGIB in this retrospective study were sourced from the MIMC-IV database. Patients were randomly allocated into training and validation cohorts for further analysis. Independent predictors for AKI were identified using least absolute shrinkage and selection operator regression and multivariable logistic regression analyses in the training cohort. Based on the logistic regression results, a nomogram was developed to predict early AKI onset in acute NVUGIB patients, and implemented as a web-based calculator for clinical application. The nomogram’s performance was evaluated through discrimination, using the C-index, calibration curves, and decision curve analysis (DCA) to assess its clinical value.

Results

The study involved 1082 acute NVUGIB patients, with 406 developing AKI. A multivariable logistic regression identified five key AKI predictors: CKD, use of human albumin, chronic liver disease, glucose, and blood urea nitrogen. The nomogram was constructed based on independent predictors. The nomogram exhibited robust accuracy, evidenced by a C-index of 0.73 in the training cohort and 0.72 in the validation cohort. Calibration curves demonstrated satisfactory concordance between predicted and observed AKI occurrences. DCA revealed that the nomogram offered considerable clinical benefit within a threshold probability range of 7% to 54%.

Conclusion

Our nomogram is a valuable tool for predicting AKI risk in patients with acute NVUGIB, offering potential for early intervention and improved clinical outcomes.
FORTCARE-MCI study protocol: evaluation of Fortasyn Connect in the management of mild cognitive impairment in primary care
Authors: Arrieta Enrique , Baz Pablo , García-Ribas Guillermo
Published: October 2024

Background

Neuropsychiatric symptoms are prevalent in patients with mild cognitive impairment (MCI) and are predictive of the conversion to dementia. Fortasyn Connect, a medical food, has shown efficacy in managing cognitive and behavioral symptoms associated with MCI. Early diagnosis and intervention in primary care are essential for managing MCI. However, real-world prospective studies assessing Fortasyn Connect in MCI are still limited.

Methods

This observational, multicenter, prospective study will enroll 150 patients recently diagnosed with MCI by primary care physicians across several regions in Spain. Participants will be followed-up over a 12-month period, with assessments at baseline, 6 months, and 12 months, as per clinical practice. The study aims to evaluate the impact of Fortasyn Connect on neuropsychiatric symptoms, cognition, and health-related quality of life (HRQoL) using validated neuropsychological tests and machine learning (ML) techniques. The primary outcome measure will be changes in neuropsychiatric symptoms using the Neuropsychiatric Inventory Questionnaire (NPI-Q) at 6 months. Secondary outcome measures include further changes in the NPI-Q at 12 months, and changes in cognition (Fototest, and clock-drawing test) and HRQoL (EQ-5D-5L) at 6 and 12 months. Exploratory outcomes will assess speech using an artificial intelligence (AI)-enhanced ML tool, with a correlation analysis of these findings with traditional neuropsychological test results.

Conclusion

This study will provide evidence of the effectiveness of Fortasyn Connect in a real-world setting, exploring its potential to stabilize or improve neuropsychiatric symptoms, cognition, and HRQoL in MCI patients. Results will also contribute to the understanding of AI and ML in identifying early biomarkers of cognitive decline, supporting the timely management of MCI.
Human sensory-like neuron cultivation—An optimized protocol
Authors: Schottmann Nicole Michelle , Grüner Julia , Bär Frederik , Karl-Schöller Franziska , Oerter Sabrina , Üçeyler Nurcan
Published: October 2024

Introduction

Reprogramming of human-induced pluripotent stem cells (iPSCs) and their differentiation into specific cell types, such as induced sensory-like neurons (iSNs), are critical for disease modeling and drug testing. However, the variability of cell populations challenges reliability and reproducibility. While various protocols for iSN differentiation exist, the development of non-iSN cells in these cultures remains an issue. Therefore, standardization of protocols is essential. This study aimed to improve iSN culture conditions by reducing the number of non-iSN cells while preserving the survival and quality of iSNs.

Methods

iSNs were differentiated from a healthy control iPSC line using an established protocol. Interventions for protocol optimization included floxuridine (FdU) or 1-β-D-arabinofuranosyl-cytosine hydrochloride (AraC) treatment, magnetic-activated cell sorting (MACS), early cell passaging, and replating. Cell viability and iSN-to-total-cell-count ratio were assessed using a luminescent assay and immunocytochemistry, respectively.

Results

Passaging of cells during differentiation did not increase the iSN-to-total-cell-count ratio, and MACS of immature iSNs led to neuronal blebbing and reduced the iSN-to-total-cell-count ratio. Treatment with high concentrations and prolonged incubation of FdU or AraC resulted in excessive cell death. However, treatment with 10 μM FdU for 24 h post-differentiation showed the most selective targeting of non-iSN cells, leading to an increase in the iSN-to-total-cell count ratio without compromising the viability or functionality of the iSN population. Replating of iSNs shortly after seeding also helped to reduce non-iSN cells.

Conclusion

In direct comparison with other methods, treatment with 10 μM FdU for 24 h after differentiation shows promise for improving iSN culture purity, which could benefit downstream applications in disease modeling and drug discovery. However, further investigations involving multiple iPSC lines and optimization of protocol parameters are warranted to fully exploit the potential of this method and enhance its reproducibility and applicability. Overall, this study provides valuable insights into optimizing culture conditions for iSN differentiation and highlights the importance of standardized protocols in iPSC-based research.
Epidemiology and tumor microenvironment of ocular surface and orbital tumors on growth and malignant transformation
Authors: Ou Shangkun , Lin Yuan , Zhang Yujie , Shi Ke , Wu Huping
Published: October 2024

The ocular surface and orbit constitute unique microenvironments in the human body. Current advances in molecular research have deepened our understanding of tumor development in these regions. Tumors exhibit greater heterogeneity compared to normal tissues, as revealed by pathological and histological examinations. The tumor microenvironment (TME) plays a crucial role in the proliferation and progression of cancer cells. Factors from the external environment or the body’s own inflammation and microcirculation interact within the TME, maintaining a delicate balance. Disruption of this balance, through uncontrolled signal pathway activation, can transform normal or benign tissues into malignant ones. In recent years, various systemic immunotherapies have been developed for cancer treatment. This study reviews the epidemiology of ocular surface and orbital tumors include squamous cell carcinoma, basal cell carcinoma, sebaceous carcinoma and lymphoma in conjunction with their occurrence, growth, and underlying mechanisms. We propose that by examining clinical histopathological images, we can identify specific and shared microscopic features of tumors. By collecting, classifying, and analyzing data from these clinical histopathological images, we can pinpoint independent diagnostic factors characteristic of tumors. We hope this study provides a basis for future exploration of the mechanisms underlying different ocular diseases.
Case report: a case of lung squamous cell carcinoma with a novel FGFR3-IER5L fusion mutation responding to anlotinib
Authors: Chen Xiaoting , Zhao Wen , Yu Hejiang , Wang Shuang , Wang Chengjun , Song Yanan , Meng Xue , Li Jisheng
Published: October 2024

Lung squamous cell carcinoma (LUSC) is the second most common pathological type of non-small cell lung cancer (NSCLC). However, compared with lung adenocarcinoma (LUAD), the incidence of driver gene mutations in LUSC is relatively lower and treatment options for LUSC patients are very limited. We described a LUSC patient with a novel FGFR3-IER5L fusion revealed by next generation sequencing in this report. The patient refused surgery, radiotherapy or chemotherapy and received anlotinib treatment. Anlotinib is a small molecular multi-target tyrosine kinase inhibitor, which can inhibit the activity of kinases including vascular endothelial growth factor receptor 2/3 (VEGFR2/3), fibroblast growth factor receptor 1-4 (FGFR1-4), platelet-derived growth factor receptor α/β (PDGFRα/β), and c-Kit. The patient achieved partial response and the progression-free survival was 3.8 months.
Conserved allomorphs of MR1 drive the specificity of MR1-restricted TCRs
Authors: Cornforth Terri V. , Moyo Nathifa , Cole Suzanne , Lam Emily P. S. , Lobry Tatiana , Wolchinsky Ron , Lloyd Angharad , Ward Katarzyna , Denham Eleanor M. , Masi Giulia , Qing Yun Phyllis Tea , Moore Colin , Dhaouadi Selsabil , Besra Gurdyal S. , Veerapen Natacha , Illing Patricia T. , Vivian Julian P. , Raynes Jeremy M. , Le Nours Jérôme , Purcell Anthony W. , Kundu Samit , Silk Jonathan D. , Williams Luke , Papa Sophie , Rossjohn Jamie , Howie Duncan , Dukes Joseph
Published: October 2024

Background

Major histocompatibility complex class-1-related protein (MR1), unlike human leukocyte antigen (HLA) class-1, was until recently considered to be monomorphic. MR1 presents metabolites in the context of host responses to bacterial infection. MR1-restricted TCRs specific to tumor cells have been described, raising interest in their potential therapeutic application for cancer treatment. The diversity of MR1-ligand biology has broadened with the observation that single nucleotide variants (SNVs) exist within MR1 and that allelic variants can impact host immunity.

Methods

The TCR from a MR1-restricted T-cell clone, MC.7.G5, with reported cancer specificity and pan-cancer activity, was cloned and expressed in Jurkat E6.1 TCRαβ− β2M− CD8+ NF-κB:CFP NFAT:eGFP AP-1:mCherry cells or in human donor T cells. Functional activity of 7G5.TCR-T was demonstrated using cytotoxicity assays and by measuring cytokine release after co-culture with cancer cell lines with or without loading of previously described MR1 ligands. MR1 allele sequencing was undertaken after the amplification of the MR1 gene region by PCR. In vivo studies were undertaken at Labcorp Drug Development (Ann Arbor, MI, USA) or Epistem Ltd (Manchester, UK).

Results

The TCR cloned from MC.7.G5 retained MR1-restricted functional cytotoxicity as 7G5.TCR-T. However, activity was not pan-cancer, as initially reported with the clone MC.7.G5. Recognition was restricted to cells expressing a SNV of MR1 (MR1*04) and was not cancer-specific. 7G5.TCR-T and 7G5-like TCR-T cells reacted to both cancer and healthy cells endogenously expressing MR1*04 SNVs, which encode R9H and H17R substitutions. This allelic specificity could be overcome by expressing supraphysiological levels of the wild-type MR1 (MR1*01) in cell lines.

Conclusions

Healthy individuals harbor T cells reactive to MR1 variants displaying self-ligands expressed in cancer and benign tissues. Described “cancer-specific” MR1-restricted TCRs need further validation, covering conserved allomorphs of MR1. Ligands require identification to ensure targeting MR1 is restricted to those specific to cancer and not normal tissues. For the wider field of immunology and transplant biology, the observation that MR1*04 may behave as an alloantigen warrants further study.
The role of ALBI score in patients treated with stereotactic body radiotherapy for locally advanced primary liver tumors: a pooled analysis of two prospective studies
Authors: Gkika Eleni , Radicioni Gianluca , Eichhorst Alexandra , Kirste Simon , Sprave Tanja , Nicolay Nils Henrik , Fichtner-Feigl Stefan , Thimme Robert , Wiehle Rolf , Brunner Thomas B. , Grosu Anca-Ligia
Published: October 2024

Introduction

To evaluate the outcomes after stereotactic body radiotherapy (SBRT) for locally advanced primary liver cancer.

Materials and methods

Patients with locally advanced liver cancer unsuitable for other loco-regional treatments were treated with SBRT with 50–60 Gy in 3–12 fractions in two consecutive prospective trials.

Results

A total of 83 patients were included, of whom 14 were excluded, leaving 69 evaluable patients with 74 treated lesions. A total of 50 patients had hepatocellular carcinoma (HCC), and 11 patients had cholangiocarcinoma (CCC). Approximately 76% had a Child-Pugh (CP) score of A, while 54% had an albumin–bilirubin (ALBI) score of 1. With a median follow-up of 29 months, the median overall survival (OS) was 11 months, and the progression-free survival (PFS) was 18 months. The ALBI score was an important predictor of overall survival (HR 2.094, p = 0.001), which remained significant also in the multivariate analysis. Patients with an ALBI grade of ≥1 had an OS of 4 months versus 23 months in patients with an ALBI grade of 1 (p ≤ 0.001). The local control at 1 and 2 years was 91%. Thirteen patients developed grade ≥ 3 toxicities, of whom nine patients experienced liver toxicities. Patients with a higher ALBI score had a high risk for developing hepatic failure (OR 6.136, p = 0.006).

Discussion

SBRT is a very effective treatment with low toxicity and should be considered as a local treatment option in patients with HCC and CCC. Patients with a higher ALBI grade are at risk for developing toxicities after SBRT and have a significantly lower survival rate.
Impaired neutrophil-mediated cell death drives Ewing’s Sarcoma in the background of Down syndrome
Authors: Peirone Serena , Tirtei Elisa , Campello Anna , Parlato Caterina , Guarrera Simonetta , Mareschi Katia , Marini Elena , Asaftei Sebastian Dorin , Bertero Luca , Papotti Mauro , Priante Francesca , Perrone Sarah , Cereda Matteo , Fagioli Franca
Published: October 2024

Introduction

Ewing Sarcoma (EWS) has been reported in seven children with Down syndrome (DS). To date, a detailed assessment of this solid tumour in DS patients is yet to be made.

Methods

Here, we characterise a chemo-resistant mediastinal EWS in a 2-year-old DS child, the youngest ever reported case, by exploiting sequencing approaches.

Results

The tumour showed a neuroectodermal development driven by the EWSR1-FLI1 fusion. The inherited myeloperoxidase deficiency of the patient caused failure of neutrophil-mediated cell death and promoted genomic instability.

Discussion

In this context, the tumour underwent genome-wide near haploidisation resulting in a massive overexpression of pro-inflammatory cytokines. Recruitment of defective neutrophils fostered rapid evolution of this EWS.
Efficacy and safety of anlotinib for triple-negative breast cancer with brain metastases
Authors: Liu Zeyu , Li Ming , Zhao Ziyi , Liu Aina , Sun Ping
Published: October 2024

Background

The anti-angiogenic agent anlotinib offers a new treatment option for triple-negative breast cancer (TNBC) patients with brain metastases. This study aimed to evaluate the efficacy and safety of anlotinib in the treatment of TNBC patients with brain metastases.

Methods

Between October 2019 and April 2024, 29 TNBC patients with brain metastases who had failed prior therapy and were treated with anlotinib were retrospectively analyzed. The primary endpoint was central nervous system (CNS) progression-free survival (PFS), and secondary endpoints included overall survival (OS), intracranial disease control rate (iDCR), intracranial objective response rate (iORR), and safety.

Results

The median CNS PFS of 29 patients was 7.2 months (95% confidence interval [CI], 3.5-10.9 months), and the median OS was 10.2 months (95% CI, 5.6-14.8 months). The iORR and iDCR were 31.0% and 86.2%, respectively. Five patients (17.2%) experienced grade 3-4 adverse events (AEs), with bone marrow suppression (2/29, 6.9%) being the most common. Most AEs were clinically manageable, and no treatment-related death was observed.

Conclusion

Anlotinib demonstrated encouraging efficacy and manageable toxicity in the treatment of TNBC patients with brain metastases who had failed standard treatment.