bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of novel blood-based extracellular vesicles biomarker candidates with potential specificity for traumatic brain injury in polytrauma patients
Authors: Schindler Cora R. , Hörauf Jason A. , Weber Birte , Schaible Inna , Marzi Ingo , Henrich Dirk , Leppik Liudmila
Published: March 2024

Objective

The goal of this study was to identify changes in extracellular vesicles (EV) surface proteins specific to traumatic brain injury (TBI), which could be used as a diagnostic and prognostic tool in polytrauma patients.

Summary Background Data

Known serum TBI-specific biomarkers (S100B, NSE, and GFAP), which can predict the severity and outcome of isolated TBI, lose their predictive value in the presence of additional extracranial injuries. Extracellular vesicles (EVs) are released from cells in response to various stimuli and carry specific cargo/surface molecules that could be used for tracking injury-responding cells.

Methods

EVs were isolated using size exclusion chromatography (SEC) from the plasma of two groups of patients (with isolated TBI, ISS≥16, AIShead≥4, n=10; and polytraumatized patients without TBI ISS≥16, AIShead=0, n=10) collected in the emergency room and 48 h after trauma. EVs’ surface epitope expression was investigated using a neurospecific multiplex flow cytometry assay and compared with healthy controls (n=10). Three enrichments of EV epitopes found to be specific to TBI were validated by western blot.

Results

The expression of 10 EV epitopes differed significantly among the patient and control groups, and five of these epitopes (CD13, CD196, MOG, CD133, and MBP) were TBI-specific. The increased expression of CD196, CD13, and MOG-positive EVs was validated by western blot.

Conclusion

Our data showed that TBI is characterized by a significant increase of CD13, CD196, MOG, CD133, and MBP-positive EVs in patients’ plasma. A high level of MOG-positive EVs negatively correlated with the Glasgow Coma Scale score at admission and could be an indicator of poor neurological status.
The interaction of lipocalin-2 and astrocytes in neuroinflammation: mechanisms and therapeutic application
Authors: Tan Qianqian , Zhang Chenxi , Rao Xiuqin , Wan Wei , Lin Wei , Huang Shupeng , Ying Jun , Lin Yue , Hua Fuzhou
Published: March 2024

Neuroinflammation is a common pathological process in various neurological disorders, including stroke, Alzheimer’s disease, Parkinson’s disease, and others. It involves the activation of glial cells, particularly astrocytes, and the release of inflammatory mediators. Lipocalin-2 (Lcn-2) is a secretory protein mainly secreted by activated astrocytes, which can affect neuroinflammation through various pathways. It can also act as a pro-inflammatory factor by modulating astrocyte activation and polarization through different signaling pathways, such as NF-κB, and JAK-STAT, amplifying the inflammatory response and aggravating neural injury. Consequently, Lcn-2 and astrocytes may be potential therapeutic targets for neuroinflammation and related diseases. This review summarizes the current knowledge on the role mechanisms, interactions, and therapeutic implications of Lcn-2 and astrocytes in neuroinflammation.
Risk level assessment of typhoon-induced wave fields around a large-scale suspended mussel farm
Authors: Li Yanjiao , Lin Jun , Zhong Wei , Wang Sijian , Li Long
Published: March 2024

Suspended shellfish aquaculture, utilizing longlines and buoys fixed on the sea surface, is a crucial source of global seafood. However, typhoons, as the most common disasters in coastal regions, can generate massive waves and pose a threat to the safety of suspended aquaculture facilities. Therefore, it is essential to investigate the risk levels associated with suspended aquaculture farms during typhoon waves. The example of a large-scale suspended mussel farm in the East China sea was examined in this study. The wave conditions under extreme wind conditions (25m/s) and different typhoon paths were predicted using the SWAN model and modeled results compared well with the observed wave and wind data. The results indicated that when the center of the east-side typhoon was located at the same latitude as the farm, it will face the highest risk level. However, for the west-side and intermediate sweeping typhoons, the risk level reached highest in the farm, when the typhoon center is lower than the latitude of it. Under the combined effects of wind fetch and topography, the risk level in the southeast farming area of the island is the highest under different wind conditions. The farms to the northwest of the island face a higher risk level during the passage of east-side sweeping typhoons, while the southern farms of the island experience an elevated risk level during the west-side and intermediate sweeping typhoons. It is suggested that farming regions with lower risk levels are more conducive to the cultivation of species with longer culture cycles. Conversely, regions considered at higher risk are more suitable for species with shorter culture cycles. The study affords a reference for the optimization and insurance of suspended aquaculture farms.
Dynamics of currents in the Qiongzhou strait during spring and summer based on a numerical simulation
Authors: Zheng Quanxin , Wang Huiqun , Li Song , Cao Zhenyi , Bao Min
Published: March 2024

A high-precision three-dimensional hydrodynamic model based on the Semi-implicit Cross-scale Hydroscience Integrated System Model (SCHISM) was constructed to investigate the characteristics of residual currents in the Qiongzhou Strait during spring and summer. Results reveal an overall westward transport in the strait during spring and summer. However, the results also support the potential occurrence of transient eastward Eulerian residual currents, which should be evident predominantly at the surface in the central and eastern sections of the strait and mainly characterised by subtidal oscillations. The direction and intensity of these residual currents indicate a pronounced influence of pressure gradient forces on both sides of the strait. The eastward flow observed in spring may represent a compensatory mechanism resulting from the relaxation period of long-term easterly winds, whereas the generation of eastward residual currents in summer is influenced by wind stress curl differences in both the western and eastern areas of the strait. The direct impact of the summer monsoon on the flow field in the strait is relatively weak. Additionally, the direction and intensity of the residual currents in the strait exhibit potential interannual variations that are directly associated with interannual wind variations. This study provides valuable insights in relation to the ongoing discourse on the dynamics of residual currents in the Qiongzhou Strait.
Endophytic Aspergillus hiratsukae mediated biosynthesis of silver nanoparticles and their antimicrobial and photocatalytic activities
Authors: Saied Ebrahim , Abdel-Maksoud Mostafa A. , Alfuraydi Akram A. , Kiani Bushra Hafeez , Bassyouni Mohamed , Al-Qabandi Osama A. , Bougafa Fathia H. E. , Badawy Mona Shaban E. M. , Hashem Amr H.
Published: March 2024

In the current study, endophytic Aspergillus hiratsukae was used for the biosynthesis of silver nanoparticles (Ag-NPs) for the first time. The characterizations were performed using X ray diffraction (XRD), Transmission electron microscopy (TEM), Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM–EDX), Dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), and UV–Vis spectroscopy. The obtained results demonstrated the successful formation of crystalline, spherical Ag-NPs with particle diameters ranging from 16 to 31 nm. The FT-IR studied and displayed the various functional groups involved, which played a role in capping and reducing agents for Ag-NPs production. The SEM–EDX revealed that the main constituent of the AS-formed sample was primarily Ag, with a weight percentage of 64.2%. The mycosynthesized Ag-NPs were assessed for antimicrobial as well as photocatalytic activities. The antimicrobial results indicated that the synthesized Ag-NPs possess notable antibacterial efficacy against Staphylococcus aureus, Bacillus subtilis, and Escherichia coli, with minimum inhibitory concentrations (MICs) of Ag-NPs ranging from 62.5 to 250 μg/mL. Moreover, the biosynthesized Ag-NPs demonstrated weak antifungal activity against Aspergillus brasiliensis and Candida albicans, with MICs of 500 and 1,000 μg/mL, respectively. In addition, the mycosynthesized Ag-NPs exhibited photocatalytic activity toward acid black 2 (nigrosine) dye under both light and dark stimulation. Notably, After 300 min exposure to light, the nigrosine dye was degraded by 93%. In contrast, 51% degradation was observed after 300 min in darkness. In conclusion, Ag-NPs were successfully biosynthesized using endophytic A. hiratsukae and also exhibited antimicrobial and photocatalytic activities that can be used in environmental applications.
Protective potential of outer membrane vesicles derived from a virulent strain of Francisella tularensis
Authors: Pavkova Ivona , Bavlovic Jan , Kubelkova Klara , Stulik Jiri , Klimentova Jana
Published: March 2024

Francisella tularensis secretes tubular outer membrane vesicles (OMVs) that contain a number of immunoreactive proteins as well as virulence factors. We have reported previously that isolated Francisella OMVs enter macrophages, cumulate inside, and induce a strong pro-inflammatory response. In the current article, we present that OMVs treatment of macrophages also enhances phagocytosis of the bacteria and suppresses their intracellular replication. On the other hand, the subsequent infection with Francisella is able to revert to some extent the strong pro-inflammatory effect induced by OMVs in macrophages. Being derived from the bacterial surface, isolated OMVs may be considered a “non-viable mixture of Francisella antigens” and as such, they present a promising protective material. Immunization of mice with OMVs isolated from a virulent F. tularensis subsp. holarctica strain FSC200 prolonged the survival time but did not fully protect against the infection with a lethal dose of the parent strain. However, the sera of the immunized animals revealed unambiguous cytokine and antibody responses and proved to recognize a set of well-known Francisella immunoreactive proteins. For these reasons, Francisella OMVs present an interesting material for future protective studies.
Global sensitivity of EEG source analysis to tissue conductivity uncertainties
Authors: Vorwerk Johannes , Wolters Carsten H. , Baumgarten Daniel
Published: March 2024

Introduction

To reliably solve the EEG inverse problem, accurate EEG forward solutions based on a detailed, individual volume conductor model of the head are essential. A crucial—but often neglected—aspect in generating a volume conductor model is the choice of the tissue conductivities, as these may vary from subject to subject. In this study, we investigate the sensitivity of EEG forward and inverse solutions to tissue conductivity uncertainties for sources distributed over the whole cortex surface.

Methods

We employ a detailed five-compartment head model distinguishing skin, skull, cerebrospinal fluid, gray matter, and white matter, where we consider uncertainties of skin, skull, gray matter, and white matter conductivities. We use the finite element method (FEM) to calculate EEG forward solutions and goal function scans (GFS) as inverse approach. To be able to generate the large number of EEG forward solutions, we employ generalized polynomial chaos (gPC) expansions.

Results

For sources up to a depth of 4 cm, we find the strongest influence on the signal topography of EEG forward solutions for the skull conductivity and a notable effect for the skin conductivity. For even deeper sources, e.g., located deep in the longitudinal fissure, we find an increasing influence of the white matter conductivity. The conductivity variations translate to varying source localizations particularly for quasi-tangential sources on sulcal walls, whereas source localizations of quasi-radial sources on the top of gyri are less affected. We find a strong correlation between skull conductivity and the variation of source localizations and especially the depth of the reconstructed source for quasi-tangential sources. We furthermore find a clear but weaker correlation between depth of the reconstructed source and the skin conductivity.

Discussion

Our results clearly show the influence of tissue conductivity uncertainties on EEG source analysis. We find a particularly strong influence of skull and skin conductivity uncertainties.
Development of auditory scene analysis: a mini-review
Authors: Calcus Axelle
Published: March 2024

Most auditory environments contain multiple sound waves that are mixed before reaching the ears. In such situations, listeners must disentangle individual sounds from the mixture, performing the auditory scene analysis. Analyzing complex auditory scenes relies on listeners ability to segregate acoustic events into different streams, and to selectively attend to the stream of interest. Both segregation and selective attention are known to be challenging for adults with normal hearing, and seem to be even more difficult for children. Here, we review the recent literature on the development of auditory scene analysis, presenting behavioral and neurophysiological results. In short, cognitive and neural mechanisms supporting stream segregation are functional from birth but keep developing until adolescence. Similarly, from 6 months of age, infants can orient their attention toward a target in the presence of distractors. However, selective auditory attention in the presence of interfering streams only reaches maturity in late childhood at the earliest. Methodological limitations are discussed, and a new paradigm is proposed to clarify the relationship between auditory scene analysis and speech perception in noise throughout development.
Higher serum Lp-PLA2 is associated with cognitive impairment in Parkinson’s disease patients
Authors: Wu Zubo , Shu Defeng , Wu Suyuan , Cai Pengcheng , Liang Tao
Published: March 2024

Objective

To explore the association between lipoprotein-associated phospholipase A2 (Lp-PLA2) and the risk of cognitive impairment in Parkinson’s disease (PD-CI).

Methods

A case–control study involving 100 hospitalized PD patients and 60 healthy controls was carried out. Serum Lp-PLA2 level was detected by automatic biochemical analyzer. Based on whether Parkinson’s patients have cognitive impairment, PD patients were subdivided to analyze the clinical value of Lp-PLA2. Relationship between Lp-PLA2 and PD-CI risk was analyzed by logistic regression. Diagnostic value of Lp-PLA2 in PD-CI patients was investigated using receiver’s operator characteristic curves.

Results

The levels of serum Lp-PLA2 activity in Parkinson’s disease with normal cognition (PD-NC) and PD-CI patients were significantly higher than those in healthy controls (HCs), respectively. Furthermore, compared to the PD-NC group, the serum Lp-PLA2 activity level was significantly higher in PD-CI patients. Multivariable logistic regression analysis indicated that higher Lp-PLA2 level was an independent risk factor for PD patients with cognitive impairment. Moreover, the area under the efficacy curve of Lp-PLA2 for predicting PD-CI is 0.659.

Conclusion

Our study shows that higher levels of Lp-PLA2 activity in PD patients are associated with the risk of developing cognitive impairment. Therefore, given the wide availability, safety, and convenience of monitoring serum Lp-PLA2 activity, it may serve as an early biomarker for cognitive impairment in PD patients.
Novel biomarkers of inflammation-associated immunity in cervical cancer
Authors: Zhao Weihong , Li Qi , Wen Songquan , Li Yaqin , Bai Ying , Tian Zhiyu
Published: March 2024

Background

Cervical cancer (CC) is a highly malignant gynecological cancer with a direct causal link to inflammation, primarily resulting from persistent high-risk human papillomavirus (HPV) infection. Given the challenges in early detection and mid to late-stage treatment, our research aims to identify inflammation-associated immune biomarkers in CC.

Methods

Using a bioinformatics approach combined with experimental validation, we integrated two CC datasets (GSE39001 and GSE63514) in the Gene Expression Omnibus (GEO) to eliminate batch effects. Immune-related inflammation differentially expressed genes (DGEs) were obtained by R language identification.

Results

This analysis identified 37 inflammation-related DEGs. Subsequently, we discussed the different levels of immune infiltration between CC cases and controls. Weighted gene co-expression network analysis (WGCNA) identified seven immune infiltration-related modules in CC. We identified 15 immune DEGs associated with inflammation at the intersection of these findings. In addition, we constructed a protein interaction network using the String database and screened five hub genes using "CytoHubba": CXC chemokine ligand 8 (CXCL8), CXC chemokine ligand 10 (CXCL10), CX3C chemokine receptor 1 (CX3CR1), Fc gamma receptors 3B (FCGR3B), and SELL. The expression of these five genes in CC was determined by PCR experiments. In addition, we assessed their diagnostic value and further analyzed the association of immune cells with them.

Conclusions

Five inflammation- and immune-related genes were identified, aiming to provide new directions for early diagnosis and mid to late-stage treatment of CC from multiple perspectives.
An in vitro study to elucidate the effects of Product Nkabinde on immune response in peripheral blood mononuclear cells of healthy donors
Authors: Setlhare Boitumelo , Letsoalo Marothi , Nkabinde Siphathimandla Authority , Nkabinde Magugu , Mzobe Gugulethu , Mtshali Andile , Parveen Sobia , Ngcobo Samukelisiwe , Invernizzi Luke , Maharaj Vinesh , Ngcobo Mlungisi , Gqaleni Nceba
Published: March 2024

Introduction: A significant number of the South African population still rely on traditional medicines (TM) for their primary healthcare. However, little to no scientific data is available on the effects of most TM products on cytokine and cellular biomarkers of the immune response. We evaluated the impact of a TM [Product Nkabinde (PN)] in inducing cellular and cytokine biomarkers of immune response in peripheral blood mononuclear cells (PBMCs).

Methods: PN, a combination of four indigenous South African plants was used in this study. The IC50 was established using the cell viability assay over 24 h. Luminex and flow cytometry assays were used to measure cytokine and cellular levels in PBMCs stimulated with PN and/or PHA over 24, 48, and 72 h, respectively. UPLC-HRMS was used to analyze an ethanol: water extract of PN to better understand the possible active compounds.

Results: The IC50 concentration of PN in treated PBMCs was established at 325.3 μg/mL. In the cellular activation assay, the percentages of CD38-HLA-DR + on total CD4+ T cells were significantly increased in PBMCs stimulated with PN compared to unstimulated controls after 24 h (p = 0.008). PN significantly induced the production of anti-inflammatory IL-10 (p = < 0.001); proinflammatory cytokines IL-1α and IL-1β (p = < 0.001), TNF-α (p < 0.0001); and chemokine MIP-1β (p = < 0.001) compared to the unstimulated control after 24 h. At 48 h incubation, the production of proinflammatory cytokines IL-1α (p = 0.003) was significantly induced following treatment with PN, and IL-10 was induced (p = 0.006). Based on the UPLC-HRMS analysis, four daphnane diterpenoids viz., yuanhuacine A (1), gniditrin (2), yuanhuajine (3) and yuanhuacine (4) were identified based on their accurate mass and fragmentation pattern.

Conclusion: The results show that PN possesses in vitro immunomodulatory properties that may influence immune and inflammatory responses. This study contributes to scientific knowledge about the immune effects of TM. More studies using PN are needed to further understand key parameters mediating induction, expression, and regulation of the immune response in the context of pathogen-associated infections.
Phytochrome phosphorylation in plant light signaling
Authors: Han Yun-Jeong , Kim Seong-Hyeon , Kim Jeong-Il
Published: March 2024

Plant phytochromes, renowned phosphoproteins, are red and far-red photoreceptors that regulate growth and development in response to light signals. Studies on phytochrome phosphorylation postulate that the N-terminal extension (NTE) and hinge region between N- and C-domains are sites of phosphorylation. Further studies have demonstrated that phosphorylation in the hinge region is important for regulating protein–protein interactions with downstream signaling partners, and phosphorylation in the NTE partakes in controlling phytochrome activity for signal attenuation and nuclear import. Moreover, phytochrome-associated protein phosphatases have been reported, indicating a role of reversible phosphorylation in phytochrome regulation. Furthermore, phytochromes exhibit serine/threonine kinase activity with autophosphorylation, and studies on phytochrome mutants with impaired or increased kinase activity corroborate that they are functional protein kinases in plants. In addition to the autophosphorylation, phytochromes negatively regulate PHYTOCHROME-INTERACTING FACTORs (PIFs) in a light-dependent manner by phosphorylating them as kinase substrates. Very recently, a few protein kinases have also been reported to phosphorylate phytochromes, suggesting new views on the regulation of phytochrome via phosphorylation. Using these recent advances, this review details phytochrome regulation through phosphorylation and highlights their significance as protein kinases in plant light signaling.
Community-forming traits play role in effective colonization of plant-growth-promoting bacteria and improved plant growth
Authors: Pathak Devashish , Suman Archna , Sharma Pushpendra , Aswini Krishnan , Govindasamy Venkadasamy , Gond Shrikant , Anshika Rana
Published: March 2024

Community-forming traits (CFts) play an important role in the effective colonization of plant-growth-promoting bacterial communities that influence host plants positively by modulating their adaptive functions. In this study, by considering plant-growth-promoting traits (PGPts) and community-forming traits (CFts), three communities were constructed, viz., SM1 (PGPts), SM2 (CFts), and SM3 (PGPts+CFts). Each category isolates were picked up on the basis of their catabolic diversity of different carbon sources. Results revealed a distinctive pattern in the colonization of the communities possessed with CF traits. It was observed that the community with CFts colonized inside the plant in groups or in large aggregations, whereas the community with only PGPts colonized as separate individual and small colonies inside the plant root and leaf. The effect of SM3 in the microcosm experiment was more significant than the uninoculated control by 22.12%, 27.19%, and 9.11% improvement in germination percentage, chlorophyll content, and plant biomass, respectively. The significant difference shown by the microbial community SM3 clearly demonstrates the integrated effect of CFts and PGPts on effective colonization vis-à-vis positive influence on the host plant. Further detailed characterization of the interaction will take this technology ahead in sustainable agriculture.
Parents’ experiences of living with a child with cancer undergoing hematopoietic stem cell transplantation: a qualitative content analysis study
Authors: Maleki Maryam , Dehghan Nayeri Nahid , Hamidieh Amir Ali , Pouraboli Batool
Published: March 2024

Objectives

Pediatric Hematopoietic Stem Cell Transplant (HSCT) profoundly impacts the physical, psychological, and social aspects of parents’ lives. Thus, this study aimed to explore the experiences of parents living with a child with cancer who undergoes HSCT.

Methods

This qualitative study involved 20 parents of children with cancer who were undergoing HSCT at a referral hospital in Iran. Purposive sampling was used to select the participants from February 2023 to November 2023. In-depth semi-structured interviews, featuring open-ended questions, were utilized for data collection. Data analysis was performed using conventional content analysis.

Results

Data analysis revealed two main themes. “Surrounded by hardships” and “Self-actualization.” The first theme encompassed participants’ experiences of facing difficulties in life after being aware of their child’s need for HSCT. This theme consisted of four categories: “uncertainty about the child’s future,” “exhaustion from the child’s treatment process,” “worrying about the healthy child(ren),” and “helplessness.” The second theme “self-actualization” included with two categories: “transformation in life’s philosophy” and “acquisition of new capabilities.” These categories highlighted the positive outcomes experienced by the participants following their child’s HSCT.

Conclusion

Our findings underscore the importance of healthcare providers being attuned to parents’ experiences throughout their child’s HSCT trajectory. It is crucial for healthcare providers to encourage parents to articulate their concerns and feelings and seek support from healthcare providers, family, and friends. The development of psychological support services in healthcare settings can facilitate tailored interventions to alleviate parents’ difficulties.
Promoting community health and climate justice co-benefits: insights from a rural and remote island climate planning process
Authors: Kennedy Angel M. , Tsakonas Kiera , Berman-Hatch Forrest , Conradi Sophia , Thaysen Max , Gillespie Manda Aufochs , Gislason Maya K.
Published: March 2024

Climate change is an environmental crisis, a health crisis, a socio-political and an economic crisis that illuminates the ways in which our human-environment relationships are arriving at crucial tipping points. Through these relational axes, social structures, and institutional practices, patterns of inequity are produced, wherein climate change disproportionately impacts several priority populations, including rural and remote communities. To make evidence-based change, it is important that engagements with climate change are informed by data that convey the nuance of various living realities and forms of knowledge; decisions are rooted in the social, structural, and ecological determinants of health; and an intersectional lens informs the research to action cycle. Our team applied theory- and equity-driven conceptualizations of data to our work with the community on Cortes Island—a remote island in the northern end of the Salish Sea in British Columbia, Canada—to aid their climate change adaptation and mitigation planning. This work was completed in five iterative stages which were informed by community-identified needs and preferences, including: An environmental scan, informal scoping interviews, attending a community forum, a scoping review, and co-development of questions for a community survey to guide the development of the Island’s climate change adaptation and mitigation plan. Through this community-led collaboration we learned about the importance of ground truthing data inaccuracies and quantitative data gaps through community consultation; shifting planning focus from deficit to strengths- and asset-based engagement; responding to the needs of the community when working collaboratively across academic and community contexts; and, foregrounding the importance of, and relationship to, place when doing community engagement work. This suite of practices illuminates the integrative solution-oriented thinking needed to address complex and intersecting issues of climate change and community health.
Correlated environmental DNA and electrofished estimates of relative biomass for golden perch, Macquaria ambigua
Authors: Rourke Meaghan L. , Broadhurst Matt K. , Fowler Ashley M. , Hughes Julian M. , DiBattista Joseph D. , Furlan Elise M.
Published: March 2024

In response to the need to augment electrofishing surveys of golden perch, Macquaria ambigua (an important endemic freshwater fish in southeastern Australia), with other non-lethal survey methods, the utility of environmental DNA (eDNA) as an index of relative fish abundance/biomass was investigated. From three adjacent rivers, five sites in each were sampled for eDNA immediately before electrofishing during sequential years. Up to six individuals or 10 kg of electrofished golden perch (200–548 mm total length) were caught or observed site−1. Analyses of concurrent eDNA concentrations revealed no significant relationship with the relative abundance of golden perch, but there was with relative biomass—manifesting as increasing eDNA concentrations between 1.1 and approximately 5.5 kg biomass site−1, after which concentrations stabilized at greater biomasses. Future research warrants assessing the viability of sampling eDNA for spatio-temporally monitoring rivers where low biomasses of golden perch (1–5 kg site−1) are likely to occur.
Exploring refugees' experience of accessing dental health services in host countries: a scoping review
Authors: Asfari Elaf , Rodriguez Andrea , Dakessian Arek , Yuan Siyang
Published: March 2024

Introduction

Refugees often face worse oral health outcomes, such as periodontal diseases and dental caries in host countries due to barriers including language and cultural differences, institutional discrimination, and restricted use of dental health services. This scoping review aims to map and summarise the available studies on refugees’ experience of accessing dental health services in the host countries, to identify the main characteristics of the dental health services that refugees access and to explore the barriers and enablers to navigate the dental health service system in their host countries.

Methods

The Joanna Briggs Institute (JBI) framework was adopted. PubMed, Scopus, Assia, CINAHL and Social Services Abstract were searched. A search strategy was developed using Medical Subject Headings (MeSH) terms and a combination of search operators and syntax used in MEDLINE were adopted for the remaining databases. Data were synthesised using thematic analysis.

Results

Fourteen articles were included. Most studies used qualitative methods and Australia seemed to be the country with the highest number of publications surrounding this topic. The included studies showed that refugees frequently encountered substantial obstacles when attempting to access dental services in host countries. Numerous barriers such as language barriers, cultural differences, and lack of health insurance or financial support hindered refugees' ability to access these services. Additionally, many refugees possessed limited knowledge of the dental care system in their new country. As a result of untreated dental problems, refugees suffered from pain and other health complications.

Discussion

This scoping review explored the challenges refugees have experienced in accessing dental health services in host countries, which included the key barriers such as affordability, accessibility, accommodation, availability, awareness, and acceptability. The scarcity of relevant research highlighted the need for a more comprehensive understanding of refugees’ experiences accessing dental health services in host countries. Limited data were identified regarding evidence focusing on the characteristics of dental services accessed by refugees in host countries.
Case report: Necrotizing leukomyelitis and meningitis in a Pomeranian
Authors: Santifort Koen M. , Garosi Laurent , Weerts Erik A. W. S.
Published: March 2024

A 2.5-year-old female entire Pomeranian dog was presented for acute paraparesis progressing within 2 days to paraplegia. General physical examination was unremarkable. Neurological examination showed paraplegia without nociception, a mass reflex upon testing perineal reflexes and withdrawal reflexes in the pelvic limbs and patellar hyperreflexia. Cutaneous trunci reflexes were absent caudal to the level of the 6th thoracic vertebra. Spinal hyperesthesia was present. Neuroanatomical localization was consistent with a T3-L3 myelopathy. Hematological and biochemical blood tests [including C-reactive protein (CRP)] were within reference ranges. MRI of the spinal cord from the level of the 1st thoracic vertebra to the sacrum revealed a patchy, ill-defined, moderate to marked T2W hyperintense, contrast enhancing intramedullary lesion extending from T1 to L4. Medical treatment based on a working diagnosis of meningomyelitis of unknown cause was initiated with corticosteroids and methadone based on pain scores. Prognosis was grave and after 3 days without return of nociception, the dog was euthanized according to the owners’ wishes. Post-mortem histopathological examination of the brain and spinal cord yielded a morphological diagnosis of severe, segmental, bilateral and fairly symmetrical, necrotizing lymphohistiocytic leukomyelitis, with a non-suppurative angiocentric leptomeningitis. Some minor, focal, lymphocytic perivascular cuffing was found in the medulla oblongata as well, but otherwise there were no signs of brain involvement. No infectious causes were identified with ancillary tests. This case report underlines the importance of including meningomyelitis in the differential diagnosis list of dogs presented for acute progressive neurological signs referable to a myelopathy.
Trust in the Transplant Team Associated With the Level of Chronic Illness Management—A Secondary Data Analysis of the International BRIGHT Study
Authors: Mielke Juliane , Cajita Maan Isabella , Denhaerynck Kris , Valenta Sabine , Dobbels Fabienne , Russell Cynthia L. , De Geest Sabina ,   the BRIGHT study team
Published: March 2024

A trustful relationship between transplant patients and their transplant team (interpersonal trust) is essential in order to achieve positive health outcomes and behaviors. We aimed to 1) explore variability of trust in transplant teams; 2) explore the association between the level of chronic illness management and trust; 3) investigate the relationship of trust on behavioral outcomes. A secondary data analysis of the BRIGHT study (ID: NCT01608477; https://clinicaltrials.gov/ct2/show/NCT01608477?id=NCT01608477&rank=1) was conducted, including multicenter data from 36 heart transplant centers from 11 countries across four different continents. A total of 1,397 heart transplant recipients and 100 clinicians were enrolled. Trust significantly varied among the transplant centers. Higher levels of chronic illness management were significantly associated with greater trust in the transplant team (patients: AOR= 1.85, 95% CI = 1.47–2.33, p < 0.001; clinicians: AOR = 1.35, 95% CI = 1.07–1.71, p = 0.012). Consultation time significantly moderated the relationship between chronic illness management levels and trust only when clinicians spent ≥30 min with patients. Trust was significantly associated with better diet adherence (OR = 1.34, 95%CI = 1.01–1.77, p = 0.040). Findings indicate the relevance of trust and chronic illness management in the transplant ecosystem to achieve improved transplant outcomes. Thus, further investment in re-engineering of transplant follow-up toward chronic illness management, and sufficient time for consultations is required.
Field Programmable Gate Array-Based Acceleration Algorithm Design for Dynamic Star Map Parallel Computing
Authors: Cui Bo , Wang Lingyun , Li Guangxi , Ren Xian
Published: March 2024

The dynamic star simulator is a commonly used ground-test calibration device for star sensors. For the problems of slow calculation speed, low integration, and high power consumption in the traditional star chart simulation method, this paper designs a FPGA-based star chart display algorithm for a dynamic star simulator. The design adopts the USB 2.0 protocol to obtain the attitude data, uses the SDRAM to cache the attitude data and video stream, extracts the effective navigation star points by searching the starry sky equidistant right ascension and declination partitions, and realizes the pipelined displaying of the star map by using the parallel computing capability of the FPGA. Test results show that under the conditions of chart field of view of Φ20° and simulated magnitude of 2.0∼6.0 Mv, the longest time for calculating a chart is 72 μs under the clock of 148.5 MHz, which effectively improves the chart display speed of the dynamic star simulator. The FPGA-based star map display algorithm gets rid of the dependence of the existing algorithm on the computer, reduces the volume and power consumption of the dynamic star simulator, and realizes the miniaturization and portable demand of the dynamic star simulator.
Model Predictive Control Strategy Based on Loss Equalization for Three-Level ANPC Inverters
Authors: Wan Shaoqi , Wang Bo , Chen Jingbo , Dong Haiying , Lv Congxin
Published: March 2024

Targeting the issue of high losses of individual switching tubes in Neutral-Point Clamped (NPC) three-level inverters, an Active Neutral-Point Clamped (ANPC) three-level inverter is used, and a model predictive control strategy using the loss equalization of the inverter is proposed. This method organizes and analyzes multiple zero-state current pathway commutation modes and adds mode three under the original two commonly used zero-state commutation modes. On this basis, the three modes are flexibly switched by model predictive control, and the output is optimized according to the value function for the space vector in each operation, while the midpoint voltage control is added to the value function. The simulation results suggest that the recommended strategy in this study may effectively realize the loss equalization control and midpoint voltage control of the ANPC inverter, which improves the operation efficiency of the electromechanical actuator.
Life Cycle Assessment of Dried Organic Apple Value Chains Considering Conventional and Heat-Pump-Assisted Drying Processes: The Case of Sweden
Authors: Bosona Techane
Published: March 2024

The increasing population pressure and demand for quality food, and the significant burden of agriculture on the environment, impede the sustainable development of the food sector. Understanding the environmental performance of different agricultural technologies and food value chains and identifying improvement opportunities play important roles in the sustainable development of this sector. This article presents the results of an environmental impact assessment of organic dried apples produced and supplied in Sweden, which was conducted using primary and literature-based data. A “cradle-to-consumer gate” life cycle analysis (LCA) method with a functional unit (FU) of 1 ton of fresh organic apples at the farm stage was used while considering conventional drying and heat-pump (HP)-assisted apple-drying techniques. The main environmental impact categories investigated were cumulative energy demand (CED), climate change impact (GWP), acidification potential (AP), and eutrophication potential (EP). The results indicate that the total CED values were 7.29 GJ and 5.12 GJ per FU for the conventional drying and HP-assisted drying methods, respectively, i.e., a reduction of about 30%. Similarly, the GWP values were 130 kg CO2 eq and 120 kg CO2 eq per FU, respectively. These findings highlight the importance of improving energy use and process efficiency to increase the sustainability of dried organic apple value chains.
Temporal Synchronization of Nitrogen and Sulfur Fertilization: Impacts on Nutrient Uptake, Use Efficiency, Productivity, and Relationships with Other Micronutrients in Soybean
Authors: Khalili Azizullah , Khalofah Ahlam , Ramesh Aketi , Sharma Mahaveer P.
Published: March 2024

Nitrogen (N) and sulfur (S) are essential nutrient elements, and their deficiency affects crop growth, productivity, and nutrient uptake due to their multifaceted role in plant metabolism, which has been well documented. Therefore, agricultural management strategies that can overcome these deficiencies are the need of the hour. In this context, a study was undertaken with the objective to assess the impacts of N and S applications, either basally or through split application (12.5, 25 and 50 kg ha−1), on the nutrient uptake, productivity, use efficiency, and micronutrient content status in soybean seeds, and also the change in soil nutrient zinc (Zn) and iron (Fe) content at different critical stages of soybean crop growth. The field trial was conducted utilizing a randomized complete-block design, and comprised fourteen treatments with varying N and S quantities. N and S were applied through basal and split applications in different combinations. The salient findings indicated that the highest seed, straw yield, N, and S uptake were obtained with the application of N25+25, S25+25, and did not significantly vary with N25+25, S12.5+12.5, N50, and N25+S50. The highest N use efficiency was recorded with the application of N25+S50, and S use efficiency with the application of N25+25, S25+25. The split application of N and S as N25+25, S25+25 significantly increased soil Zn and Fe content at R2 and R5 stages of soybean crop growth, as well as seed Zn and Fe uptake. It can be concluded that the basal and split application of N and S at the rate of 25 kg ha−1 can improve soybean productivity through increased mobilization and assimilation by plants. The findings indicated that applying N and S separately, with 25 kg ha−1 each basally and at the R2 stage resulted in the highest nutrient uptake, and seed and straw yields. The nutrient use efficiencies, along with Zn and Fe uptake by seeds, exhibited noticeable improvements with this split application approach compared to the control. Furthermore, the soil Zn and Fe contents also experienced enhancements due to the split application of both Nand S fertilizers. These results underscore the potential benefits of temporally adopting optimized fertilizer application strategies to maximize agricultural productivity while ensuring efficient nutrient utilization and soil health maintenance. Further research and field trials could provide deeper insights into the long-term impacts and scalability of this approach across different crop varieties and environmental conditions.
Additive Impacts of Liveweight and Body Condition Score at Breeding on the Reproductive Performance of Merino and Non-Merino Ewe Lambs
Authors: Thompson Andrew N. , Ferguson Mark B. , Kearney Gavin A. , Kennedy Andrew J. , Kubeil Lyndon J. , Macleay Claire A. , Rosales-Nieto Cesar A. , Paganoni Beth L. , Trompf Jason P.
Published: March 2024

Ewe lambs that are heavier due to improved nutrition pre- and post-weaning achieve puberty at a younger age, are more fertile, and have a higher reproductive rate. Fatness is intimately linked to reproduction, and we hypothesised that higher body condition scores at breeding would have positive effects on the reproductive rate of ewe lambs over and above liveweight. We also expected that if only a proportion of ewe lambs were presented for breeding, then it would be more effective to select them based on both liveweight and body condition score. To test these hypotheses, we analysed data from over 17,000 records from Merino and non-Merino ewe lambs from 22 different flocks across Australia. Non-Merino ewe lambs were more fertile (69.4% vs. 48.7%) and achieved a higher reproductive rate than Merino ewe lambs (96.9% vs. 60.7%). There were significant curvilinear relationships between liveweight (p < 0.001) or body condition score (p < 0.001) prior to breeding and reproductive rate for both Merino and non-Merino ewe lambs. For both breeds, there was a significant (p < 0.001) quadratic effect of body condition score prior to breeding on reproductive rate, independent of the correlated changes in liveweight, and at the same liveweight, an extra 0.5 of a body condition score up to 3.3 improved reproductive rate by about 20%. Nevertheless, the results indicated that if only a proportion of ewe lambs were selected for breeding, then selection based on both liveweight and body condition scores may only improve the overall reproductive rate by 1 to 2% compared to selection based on liveweight alone. We conclude that liveweight is a more effective method than body condition score for selecting ewe lambs for breeding.
Estimating Mineral Requirements of Wild Herbivores: Modelling Arctic Caribou (Rangifer tarandus granti) in Summer
Authors: Oster Keith W. , Gustine David D. , Smeins Fred E. , Barboza Perry S.
Published: March 2024

Mineral requirements are poorly described for most wildlife. Consequently, the role of forage minerals in movement and productivity are poorly understood for sedentary and migratory ungulates, such as reindeer and caribou (Rangifer tarandus). We applied estimates of maintenance, lactation, body mass change, and antler growth to production curves (body mass, daily intake, and milk yield) for female caribou to calculate their mineral requirements over summer. The total requirements (mg or g·d−1) were divided by the daily intake (kg·d−1) to estimate the minimum concentration of minerals required in the diet (mg or g·kg−1) to balance demand. The daily requirements (mg·d−1) of all minerals increased from parturition to the end of summer. The minimum dietary concentrations (mg·kg−1) of macro-minerals (Ca, P, Mg, Na, K) declined as food intake (kg·d−1) increased over summer. The minimum dietary concentrations (Fe, Mn, Cu, Zn) were heavily influenced by body mass gain, which increased through late lactation even though food intakes rose. Our modeling framework can be applied to other wild ungulates to assess the impacts of changing forage phenology, plant community compositions, or environmental disturbances on movement and productivity.