bluuebunny/crossref_metadata_2025 huggingface dataset:
Predicting rectal cancer prognosis from histopathological images and clinical information using multi-modal deep learning
Authors: Xu Yixin , Guo Jiedong , Yang Na , Zhu Can , Zheng Tianlei , Zhao Weiguo , Liu Jia , Song Jun
Published: April 2024

Objective

The objective of this study was to provide a multi-modal deep learning framework for forecasting the survival of rectal cancer patients by utilizing both digital pathological images data and non-imaging clinical data.

Materials and methods

The research included patients diagnosed with rectal cancer by pathological confirmation from January 2015 to December 2016. Patients were allocated to training and testing sets in a randomized manner, with a ratio of 4:1. The tissue microarrays (TMAs) and clinical indicators were obtained. Subsequently, we selected distinct deep learning models to individually forecast patient survival. We conducted a scanning procedure on the TMAs in order to transform them into digital pathology pictures. Additionally, we performed pre-processing on the clinical data of the patients. Subsequently, we selected distinct deep learning algorithms to conduct survival prediction analysis using patients’ pathological images and clinical data, respectively.

Results

A total of 292 patients with rectal cancer were randomly allocated into two groups: a training set consisting of 234 cases, and a testing set consisting of 58 instances. Initially, we make direct predictions about the survival status by using pre-processed Hematoxylin and Eosin (H&E) pathological images of rectal cancer. We utilized the ResNest model to extract data from histopathological images of patients, resulting in a survival status prediction with an AUC (Area Under the Curve) of 0.797. Furthermore, we employ a multi-head attention fusion (MHAF) model to combine image features and clinical features in order to accurately forecast the survival rate of rectal cancer patients. The findings of our experiment show that the multi-modal structure works better than directly predicting from histopathological images. It achieves an AUC of 0.837 in predicting overall survival (OS).

Conclusions

Our study highlights the potential of multi-modal deep learning models in predicting survival status from histopathological images and clinical information, thus offering valuable insights for clinical applications.
Case report: Ultrasound diagnosis of a complicated case of gastric lymphoma misdiagnosed as cirrhosis
Authors: Yang Dayan , Gao Lini
Published: April 2024

The gastrointestinal (GI) tract is the most common primary site for extranodal lymphomas. The use of ultrasonography for diagnosing gastric lymphomas can be challenging, but ultrasonography still offers some unique advantages in the diagnosis of GI lymphomas. Here, we report a case of gastric lymphoma in a patient with an extensive lesion in which the tumor was complexed with the abdominal organs. CT and endoscopy failed to definitively diagnose the condition in a timely manner. The gastric lymphoma was finally diagnosed with ultrasonography and a treatment plan was implemented.
Efficacy and safety of Shen Gui capsules for chronic heart failure: a systematic review and meta-analysis
Authors: Yan Jiaqi , Zhang Chaorong , Wang Yuanping , Yan Xia , Jin Lili
Published: April 2024

Background

Although Shen Gui capsules (SGCP) are widely used as an adjuvant treatment for chronic heart failure (CHF), their clinical efficacy and safety remain controversial.

Purpose

To assess the efficacy and safety of SGCP in the treatment of CHF through a systematic review and meta-analysis, to provide high-quality evidence for evidence-based medicine.

Methods

Seven databases were searched for randomized controlled trials (RCTs) assessing SGCP for CHF, from inception to 9 January 2023. RCT quality of evidence was evaluated using the Cochrane Handbook for the Evaluation of Intervention Systems to assess risk of bias and Grading of Recommendations Assessment, Development, and Evaluation. A meta-analysis with subgroup and sensitivity analyses was performed using Review Manager 5.4 and Stata 12.

Results

Nine RCTs representing 888 patients with CHF were included in the review. Meta-analysis revealed that SGCP combined with conventional heart failure therapy is more advantageous for improving left ventricular ejection fraction [LVEF; mean difference (MD) = 5.26, 95% confidence interval (CI) (3.78, 6.74), p < 0.0000] and increasing effective rate [relative risk (RR) = 1.21, 95%CI (1.14, 1.29), p < 0.001] compared with conventional therapy alone. The experimental treatment also reduced brain natriuretic peptide [MD = −100.15, 95%CI (−157.83, −42.47), p = 0.0007], left ventricular end-diastolic diameter [MD = −1.93, 95%CI (−3.22, −0.64), p = 0.003], and hypersensitive C-reactive protein [MD = −2.70, 95%CI (−3.12,−2.28), p < 0.001] compared with the control group. However, there was not a statistically significant difference in tumor necrosis factor-α [MD = −14.16, 95%CI (−34.04, 5.73), p = 0.16] or left ventricular end-systolic diameter [MD = −1.56, 95%CI (−3.13, 0.01), p = 0.05]. Nor was there a statistically significant between-groups difference in incidence of adverse events (p > 0.05).

Conclusion

SGCP combined with conventional heart failure therapy can improve LVEF and increase the effective rate to safely treat patients with CHF. However, further high-quality studies are needed to confirm these findings, due to the overall low quality of evidence in this literature.

Clinical Trial Registration:

https://www.crd.york.ac.uk/PROSPERO/logout.php, PROSPERO [CRD42023390409].
Xinmailong injection on left ventricular remodeling and inflammatory mediators in patients with CHF: a systematic review and meta-analysis
Authors: Han Xu , Chen Xi , Liu Yanling , Yang Jie , Nie Wuzhi , Yang Mingjiu , Mou Xinglang
Published: April 2024

Background:

Chronic heart failure (CHF) is a prevalent and highly challenging cardiovascular disease associated with high mortality rates. The occurrence and progression of CHF are closely linked to left ventricular remodeling (LVR) and inflammation. Addressing LVR and reducing inflammation can significantly slow down the progression of CHF and improve patient prognosis.

Objective:

To evaluate the effects of Xinmailong injection (XMLI) on LVR and inflammatory mediators in CHF patients.

Method:

The randomized controlled trials investigating the effectiveness of XMLI treatment for CHF were retrieved from eight databases up until 31 December 2023. To evaluate the methodological quality of included studies, the Cochrane bias risk tool was employed. Furthermore, statistical analysis, sensitivity analysis, and publication bias assessment were conducted using Stata 17.0 software.

Result:

Compared with conventional treatment (CT), the combination therapy of XMLI and CT significantly improved LVR and reduced inflammatory mediators, mainly manifested by an increase in LVEF (MD = 6.40, 95% CI: 5.25 to 7.55, p = 0.000), a decrease in LVEDD (MD = −4.63, 95% CI: −5.69 to −3.57, p = 0.000) and LVESD (MD = −4.00, 95% CI: −5.50 to −2.50, p = 0.000), as well as a decrease in TNF-α (MD = −7.93, 95% CI: −9.86 to −6.00, p = 0.000), IL-6 (MD = −5.25, 95% CI: −6.59 to −3.92, p = 0.000), IL-18 (MD = −36.07, 95% CI: −46.76 to −25.38, p = 0.000), CRP (MD = −4.41, 95% CI: −6.40 to −2.42, p = 0.000), hs-CRP (MD = −4.90, 95% CI: −5.71 to −4.08, p = 0.000), and an increase in IL-10 (MD = 20.19, 95% CI: 10.42 to 29.97, p = 0.000). In addition, the combination therapy showed enhanced clinical efficacy (OR = 4.08, 95% CI: 3.10 to 5.37, p = 0.000), decreased expression levels of BNP (MD = −138.48, 95% CI: −155.48 to −121.48, p = 0.000), and NT-pro BNP (MD = −315.63, 95% CI: −359.25 to −272.00, p = 0.000), and increased the 6-MWD (MD = 71.02, 95% CI: 57.23 to 84.81, p = 0.000). It is noteworthy that the combination therapy did not lead to an increase in the incidence of adverse reactions (OR = 1.01, 95% CI: 0.68 to 1.50, p = 0.97).

Conclusion:

This systematic review and meta-analysis demonstrated the superiority of combining XMLI and CT therapies over CT alone in improving LVR and reducing inflammatory mediators in patients with CHF. Importantly, this combination therapy does not increase adverse reactions. However, it is crucial to exercise caution while interpreting the survey results due to the limited quality of the included studies.

Systematic Review Registration:

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=492715, Identifier CRD42023492715.
C-terminally encoded peptide-like genes are associated with the development of primary root at qRL16.1 in soybean
Authors: Kumawat Giriraj , Cao Dong , Park Cheolwoo , Xu Donghe
Published: April 2024

Root architecture traits are belowground traits that harness moisture and nutrients from the soil and are equally important to above-ground traits in crop improvement. In soybean, the root length locus qRL16.1 was previously mapped on chromosome 16. The qRL16.1 has been characterized by transcriptome analysis of roots in near-isogenic lines (NILs), gene expression analysis in a pair of lines contrasting with alleles of qRL16.1, and differential gene expression analysis in germplasm accessions contrasting with root length. Two candidate genes, Glyma.16g108500 and Glyma.16g108700, have shown relatively higher expression in longer root accessions than in shorter rooting accessions. The C-terminal domain of Glyma.16g108500 and Glyma.16g108700 is similar to the conserved domain of C-terminally encoded peptides (CEPs) that regulate root length and nutrient response in Arabidopsis. Two polymorphisms upstream of Glyma.16g108500 showed a significant association with primary root length and total root length traits in a germplasm set. Synthetic peptide assay with predicted CEP variants of Glyma.16g108500 and Glyma.16g108700 demonstrated their positive effect on primary root length. The two genes are root-specific in the early stage of soybean growth and showed differential expression only in the primary root. These genes will be useful for improving soybean to develop a deep and robust root system to withstand low moisture and nutrient regimes.
Operational criteria application does not change clinicians’ opinion on the diagnosis of mental disorder: a pre- and post-intervention validity study
Authors: Rocha Neto Helio G. , Lessa José Luiz Martins , Koiller Luisa Mendez , Pereira Amanda Machado , Gomes Bianca Marques de Souza , Veloso Filho Carlos Linhares , Casado Telleria Carlos Henrique , Cavalcanti Maria T. , Telles-Correia Diogo
Published: April 2024

Objective

Our objective was to check if the ICD-10 operational criteria application changes non-operational, prototype-based diagnoses obtained in a real-life scenario.

Methods

Psychiatry residents applied the diagnostic criteria of the ICD-10 as a “diagnostic test” to five outpatient patients they were already following who had a prototype-based diagnosis. Tests were used to ascertain whether changes in opinion were significant and if any of the diagnostic groups were more prone to change than others. The present paper is part of the study with UTN U1111-1260-1212.

Results

Seventeen residents reviewed their last five case files, retrieving 85 diagnostic pairs of non-operational-based vs. operational-based diagnoses. The Stuart–Maxwell test did not indicate a significant opinion change (χ2 = 5.25, p = 0.39; power = 0.94) besides 30% of diagnostic changes. Despite not being statistically significant, 20.2% of all evaluations resulted in a change that would affect treatment choices. Using ICD-10 operational criteria slightly increased the number of observed diagnoses, but probably without clinical relevance. None of the non-operational diagnoses have a higher tendency to change with operational criteria application (χ2 = 11.6, p = 0.07). The female gender was associated with a higher diagnostic change tendency.

Conclusion

Applying ICD-10 operational criteria as a diagnostic test does not induce a statistically significant diagnostic opinion change in residents and no diagnostic group seems more sensible to diagnostic change. Gender-related differences in diagnostic opinion changes might be evidence of sunk cost bias. Although not statistically significant, using operational criteria after diagnostic elaboration might help to deal with subjects without adequate treatment response.
Eye Movement Desensitisation and Reprocessing (EMDR) therapy for prolonged grief: theory, research, and practice
Authors: Spicer Liam
Published: April 2024

Prolonged Grief Disorder occurs within 7-10% of the bereaved population and is a more complicated and persistent form of grief which has been associated with suicidality, mental health disorders, sleep disturbance, poor health behaviors, and work and social impairment. EMDR is a fitting treatment option for those with Prolonged Grief, focusing on processing past memories, blocks, current triggers, future fears, and preparing the person for living life beyond the loss in line with the Adaptive Information Processing Model and grief frameworks. This paper discusses the theory, research regarding the application of EMDR with prolonged grief, and gives insight and guidance to clinicians working in this area including a case example.
Burnout and coping mechanisms among healthcare professionals in central Uganda
Authors: Kabunga Amir , Kigongo Eustes , Okalo Ponsiano , Udho Samson , Grace Anna Auma , Tumwesigye Raymond , Akello Anne Ruth , Musinguzi Marvin , Acup Walter , Nabaziwa Jannat , Shikanga Enos Mwirotsi , Namata Haliama
Published: April 2024

Background

The escalating global prevalence of burnout among healthcare professionals poses a serious health concern. Recent studies focus on prevalence and predictors of burnout among healthcare providers, emphasizing the need for well-being interventions. This study investigates burnout and coping mechanisms among healthcare professionals in central Uganda, addressing the dearth of knowledge about coping strategies specific to the region.

Methods

An analytical facility cross-sectional study was conducted in five healthcare facilities in central Uganda between June to July 2023. Participants included physicians, nurses, and technicians actively engaged in direct patient care. Data were collected using socio-demographic surveys, the Professional Quality of Life (ProQOL-5), and the Brief-COPE tools.

Results

The study revealed a high prevalence of burnout, with 39.8% of participants experiencing significant levels. Active coping, positive reframing, and denial were negatively correlated with low burnout levels. Dysfunctional coping, specifically self-distraction and denial, showed positive correlations with average and high burnout levels. Emotion-focused coping mechanisms were not employed across burnout levels.

Conclusions

The results emphasize the demanding nature of healthcare roles in the region and highlight the need for comprehensive, context-specific interventions to address burnout globally. While some healthcare professionals utilized adaptive strategies such as seeking social support, engaging in self-care activities, and utilizing problem-solving skills, others resorted to maladaptive coping mechanisms such as substance use and avoidance behaviors. This dichotomy highlights the need for targeted interventions to promote adaptive coping strategies and mitigate the negative impact of maladaptive behaviors on individual well-being and patient care.
Prevalence of hypertension and related risk factors in older Chinese population: a meta-analysis
Authors: Wang Zicheng , Wang Shengjie , Lin Haiyan , Wang Congling , Gao Da
Published: April 2024

Objective

Hypertension is the most challenging public health problem worldwide and seriously affects human health. To date, there are no epidemiological studies on the prevalence of and risk factors for hypertension among older people in mainland China.

Methods

We conducted a meta-analysis of the prevalence and risk factors of hypertension among the older population in mainland China. We searched Chinese and English databases for Chinese and English literature on hypertension epidemiology published between 2000 and 2022, and hypertension data among the older population were extracted from the included literature. A meta-analysis was performed using a random-effects model (I2 > 50%) with 95% confidence intervals for the forest plots. Data were processed using RevMan 5.3. Forty-nine publications (with data from 84,429 samples) met the evaluation criteria and were included in this study.

Results

We found that the total prevalence of hypertension was 47%. The total prevalence rate of the older population in China from 2000 to 2010 was 50%, and the prevalence rate from 2011 to 2021 was 45%, with no significant differences. The total prevalence in Central China was the highest (59%). There was no significant correlation between the prevalence rate of the older population, sex, and urban or rural areas.

Conclusion

Hypertension is common among the older population in China, and its control rate is low. Therefore, effective prevention and treatment measures, as well as education, should be formulated to improve the diagnosis and treatment of hypertension in the older population.
Does the increase in health insurance benefits have different effects on the health of middle-aged and older people individuals in rural areas? Analysis based on quantile difference-in-differences method
Authors: Qin Cheng , Wang Xueyi
Published: April 2024

In the context of healthy aging, enhancing health performance is an intrinsic requirement for the development and reform of the health insurance system. This paper mainly discusses the health effects of increasing medical insurance benefits on people with different levels of health. So this paper utilizes multiple rounds of data from the China Health and Retirement Longitudinal Study (CHARLS) and employs the quantile difference-in-differences method to systematically investigate the impact effects of the integration of urban and rural residents' health insurance on the frailty levels of rural middle-aged and older people individuals. The research findings are as follows: Firstly, the integration of urban and rural resident health insurance has mitigated the frailty level of rural older people individuals, with a more pronounced impact on those with poorer health statuses. Secondly, in terms of heterogeneity analysis, the health performance effects of the urban-rural health insurance integration policy are more significant among the older people population and in the western regions. Thirdly, the integration of urban and rural resident health insurance primarily improves health by reducing the burden of medical expenses, with a greater impact on the older people population with poorer health statuses. Based on the research findings, we recommend addressing the disparities in healthcare benefits across various insurance systems, alleviating the financial burden of healthcare for impoverished individuals, and consistently improving the coordination of healthcare insurance policies for both urban and rural residents.
Study on the impact of COVID-19 pandemic on the mental health of Chinese college students: a cross-sectional analysis
Authors: Song Xiaodong , Han Demin , Zhang Jiaqi , Fan Jiajun , Ning Peishan , Peng Yong
Published: April 2024

Background

The COVID-19 pandemic has significantly impacted the mental health of college students, prompting the need for universities to implement measures to mitigate these adverse effects. This study aims to assess the mental health status and mitigation measures of college students, identify the primary factors contributing to their mental health challenges, and provide suggestions for educational institutions to reduce negative psychological impacts.

Methods

In February 2023, a questionnaire survey was conducted among 1,445 college students. Statistical analysis was performed on the survey results, and multiple regression models were used to identify significant influencing factors and optimize the model.

Results

The study revealed correlations between factors affecting mental health during the pandemic, with interactions observed among some factors. Significant differences in mental health status were found among different groups of college students based on their information-sharing habits through apps and engagement in thesis research. Multiple regression analysis indicated that conducting academic research related to COVID-19 significantly increased the psychological stress of college students during the pandemic (p = 0.043). Among all mitigation measures, playing games demonstrated significant effectiveness in model analysis (p = 0.047). The optimization of the model showed that the multiple regression model considering the interaction of factors was more effective.

Conclusion

Our research identifies crucial factors influencing the mental health of college students and investigates the mental health status of various student groups. We recommend that educational institutions adopt proactive strategies and a multifaceted approach to support the mental health of college students and address potential issues that may arise.
Dietary intake of polish organic and conventional fruit growers and their life partners – a pilot study
Authors: Dobrowolski Hubert , Szumigaj Bartosz , Włodarek Dariusz , Kazimierczak Renata , Obidzińska Justyna , Rembiałkowska Ewa
Published: April 2024

Diet is one of the elements that contribute to health and quality of life. There are significant discrepancies between the diets of people living in different regions, with different beliefs, or with different approaches to sustainability and ecology. There is a lack of research on dietary intake among organic and conventional fruit growers. The aim of our study was to examine the diets of orchardists and their immediate life partners in terms of meeting energy requirements, nutrient intake and fulfillment of dietary recommendations in this group. Fifty-three participants (28 in the organic group and 25 in the conventional group) took part in the study. Dietary data were obtained using the 3-day dietary record. Body mass and height were measured and BMI was calculated. Physical activity was estimated using a questionnaire method. The study group was aged 44 ± 8 years, with a body weight of 84 ± 16 kg and a height of 172 ± 9 cm. The mean BMI was 28 ± 4 kg/m2. Mean energy intake with diet was 2170 ± 606 kcal/day with needs of 3002 (1991–5144) kcal/day. A significant proportion of the study group did not fulfill their calcium and vitamin D requirements. In addition, a significant proportion of the conventional fruit growers did not cover their needs for potassium, magnesium and vitamins: E, C, and folate. Both groups had too high an intake of cholesterol and saturated fatty acids, and too low an intake of polyunsaturated fatty acids. In conclusion, the orchardists' diets mostly provided adequate amounts of nutrients, with inadequate intakes of calcium, vitamin D, cholesterol, and fatty acids. The diets of organic fruit growers were significantly richer in selected nutrients.
Seroprevalence and molecular detection of brucellosis among Pakistani women with spontaneous abortion
Authors: Ejaz Mohammad , Ali Shahzad , Syed Muhammad Ali , Melzer Falk , Faryal Rani , Dadar Maryam , Abbasi Shahid Ahmed , El-Adawy Hosny , Neubauer Heinrich
Published: April 2024

Background

Human brucellosis is a neglected disease transmitted to humans from animals such as cattle, goats, dogs, and swine. The causative agents are bacteria of the genus Brucella, intracellular pathogens usually confined to the reproductive organs of their animal hosts causing sterility and abortions. The objective of the study was to determine the seroprevalence of brucellosis among women with spontaneous abortions (SAW) and compare this seroprevalence with that of healthy pregnant women (HPW).

Methods

The case–control study was designed to determine the seroprevalence and molecular detection of brucellosis in women who suffered from spontaneous abortion and healthy pregnant women of the Haripur District of Pakistan. A total of 770 blood samples (n = 385 for each group) were collected from 9 public and 11 private hospitals in Haripur District from December 2021–March 2023. Data on demographic features, epidemiological variables, and risk factors were collected from each participant by structured questionnaires. Initial screening for brucellosis was performed by Rose Bengal Plate Test followed by qRT-PCR for molecular detection of the genus-specific BCSP-31 gene of Brucella.

Results

The study showed that anti-Brucella antibodies were more found in SAW 23.63% (91/385) than in HPW 1.29% (5/385). Brucella specific DNA was amplified in 89.01% (81/91) seropositive samples of SAW. Demographic features and risk factors such as age, urbanicity, socioeconomic status, education, occupation, and animal contact were found significantly associated with brucellosis (p ≤ 0.05). Consumption of unpasteurized raw milk (OR = 18.28, 95%CI: 8.16–40.94) was found highly concomitant with seroprevalence.

Conclusion

This study reports the first evidence of involvement of brucellosis in spontaneous abortions in women of Pakistan. The study can be used to develop strategies for risk management during pregnancy, to raise awareness for brucellosis, and develop control programs.
Measurement and influencing factor analysis of the matching degree between agricultural scientific and technology innovation and rural industry integration under the sustainable background
Authors: Yao Sheng , Wang Yuanyuan , Wu Guosong
Published: April 2024

Agricultural scientific and technological innovation and rural industry integration are the key contents of promoting agricultural and rural modernization in China. This study empirically analyzed the matching relationship between the two and its influencing factors, and found that the matching degree between agricultural scientific and technological innovation and rural industry integration in China showed a steady upward trend in the sample period. Both China as a whole and all regions completed the upgrading from moderate to high matching in the sample period, The performance of each region is also excellent. In 2020, the matching degree of 28 regions will enter a high matching range. The implementation of the rural revitalization policy will help to improve the matching degree between China’s agricultural scientific and technological innovation and rural industrial integration. The scale of rural financial development and the matching degree of lagging one period also have a significant positive impact on the improvement of the matching degree. This study further proposes relevant policy recommendations from improving the supply level of agricultural scientific and technology innovation, enhancing the adaptability between supply and demand of agricultural scientific and technology innovation, improving the policy support system, and optimizing the financial security system.
A live attenuated Salmonella Typhimurium vaccine dose and diluent have minimal effects on the caecal microbiota of layer chickens
Authors: Khan Samiullah , McWhorter Andrea R. , Andrews Daniel M. , Underwood Gregory J. , Moore Robert J. , Van Thi Thu Hao , Gast Richard K. , Chousalkar Kapil K.
Published: April 2024

Among the Salmonella reduction strategies in poultry production, one option is to use a Salmonella vaccine. The aim of vaccinating layer flocks is to reduce the shedding of wild-type Salmonella in the poultry environment, thereby reducing the contamination of poultry products (eggs and meat). Nutritive diluent and a higher dose of vaccine may enhance its colonization potential in the gut of chickens. In this study, a commercially available live attenuated vaccine (Vaxsafe® ST) was reconstituted in different media and delivered orally to day-old chicks at three different doses (107, 108, and 109 CFU/chick). Gut colonization of the vaccine strain and the effects of vaccination on gut microbiota were assessed in commercial-layer chickens. The vaccine diluent and dosage minimally affected microbiota alpha diversity. Microbiota beta diversity was significantly different (P < 0.05) based on the vaccine diluent and dose, which indicated that the vaccinated and unvaccinated chickens had different gut microbial communities. Differences were noted in the abundance of several genera, including Blautia, Colidextribacter, Dickeya, Enterococcus, Lactobacillus, Pediococcus, and Sellimonas. The abundance of Colidextribacter was significantly lower in chickens that received vaccine reconstituted in Marek's and water diluents, while Lactobacillus abundance was significantly lower in the water group. The highest vaccine dose (109 CFU/chick) did not significantly alter (P > 0.05) the abundance of microbial genera. Chicken age affected the microbiota composition more significantly than the vaccine dose and diluent. The abundance of Lactobacillus, Blautia, Caproiciproducens, Pediococcus, and Colidextribacter was significantly higher on day 14 compared with day 7 post-vaccination. The Salmonella Typhimurium vaccine load in the caeca was not significantly affected by diluent and vaccine dose; however, it was significantly lower (P < 0.0001) on day 14 compared with day 7 post-vaccination. Overall, the S. Typhimurium vaccine minimally affected the gut microbiota structure of layer chicks, whereas changes in microbiota were more significant with chicken age.
Discrete Multiwalled Carbon Nanotubes for Versatile Intracellular Transport of Functional Biomolecular Complexes
Authors: Castillo Kevin , Tasset Aaron , Marinkovic Milos , Foote Aaron
Published: April 2024

In recent years, carbon nanotubes have emerged as a potentially revolutionary material with numerous uses in biomedical applications. Compared to other nanoparticles, discrete multiwalled carbon nanotubes (dMWCNTs) have been shown to exhibit advantageous characteristics such as a high surface area-to-volume ratio, biocompatibility, and unique chemical and physical properties. dMWCNTs can be modified to load various molecules such as proteins and nucleic acids and are capable of crossing the cell membrane, making them attractive delivery vehicles for biomolecules. To investigate this, we measured the impact of dMWCNTs on the number of live and dead cells present during different stages of cell proliferation. Furthermore, we used transmission electron microscopy to produce evidence suggesting that dMWCNTs enter the cytoplasm of mammalian cells via an endocytosis-like process and ultimately escape into the cytoplasm. And lastly, we used live-cell staining, qPCR, and a T-cell activation detection assay to quantify the use of dMWCNTs as a delivery vehicle for a toxic, membrane-impermeable peptide, mRNA, siRNA, and a T-cell activating synthetic dsRNA. We demonstrate successful delivery of each payload into a range of cell types, providing further evidence of dMWCNTs as a versatile delivery platform for biomolecular cargo.
Suicidality and Non-Suicidal Self-Injury: A Narrative Review of Measurement, Risk, and Disparities among Minoritized and System-Involved Youth in the USA
Authors: Villodas Melissa L.
Published: April 2024

Suicidality and non-suicidal self-injury (NSSI) among youth in the United States continue to be a growing and serious public health concern. With alarming rates of suicide trending in the wrong direction, researchers are committed to bending the curve of suicide and reducing rates by 2025. Understanding the antecedents and conditions, existing measures, and disparate prevalence rates across minoritized groups is imperative for developing effective strategies for meeting this goal. This study presents a narrative review of the operationalization, measurement, risk factors (e.g., firearms and social media), and disparities across race, ethnicity, age, gender identity, ability, sexual orientation, immigration statuses, and system involvement (e.g., foster care and juvenile justice) of suicidality and non-suicidal self-harm across youth in the United States. Implications for research, practice, and policy approaches that incorporate positive youth development, cultural, and youth participation in interventions are discussed.
Bracing of Pectus Carinatum in Children: Current Practices
Authors: Omanik Pavol , Sesia Sergio Bruno , Kozlikova Katarina , Schmidtova Veronika , Funakova Miroslava , Haecker Frank-Martin
Published: April 2024

Background: Although effective, compressive orthotic bracing (COB) in children with pectus carinatum is still not standardized. This study has aimed to analyze current practices amongst members of the Chest Wall International Group (CWIG). Methods: A web-based questionnaire was mailed to all CWIG members at 208 departments. It included 30 questions regarding diagnostic work-up, age for COB indication, type of COB used, daily wearing time, treatment duration, complications, and recurrence rate. Results: Members from 44 departments have responded (institutional response rate 21.2%). A total of 93% consider COB as the first-line treatment for PC. A conventional COB (CC) is used in 59%, and the dynamic compression system (FMF) in 41%. The overall compliance rate is >80%. A total of 67% of responders consider COB to be indicated in patients <10 years. The actual wearing time is significantly shorter than the physician-recommended time (p < 0.01). FMF patients experience a significantly faster response than CC patients (p < 0.01). No recurrence of PC has been noted in 34%; recurrence rates of 10–30% have been noted in 61%. Conclusions: COB is the first-line treatment for PC with a high compliance rate. During puberty, the recurrence rate is high. Treatment standardization and follow-up until the end of puberty are recommended to enhance COB effectiveness.
Responding to the Current Capricious State of Australian Educational Leadership: We Should Have Seen It Coming!
Authors: Branson Christopher M. , Marra Maureen , Kidson Paul
Published: April 2024

The capricious state of Australian educational leadership is evidenced in the publication, “The Australian Principal Occupational Health, Safety, and Wellbeing Survey 2022 Data”, which highlights unsustainable adverse health outcomes for an increasing number of school leaders. According to this report, the accumulation of stress caused by the sheer quantity of work, the lack of time to focus on teaching and learning, a lack of sufficient teachers, and having to care for an increasing number of staff and students with mental health issues were the main causes of professional disillusionment and burnout among Australian school leaders. Moreover, the level of destabilisation and chaos that this situation could cause, should it continue to rise, is compounded by current research highlighting an ever-decreasing number of applicants for school leadership positions. To assign blame for this serious predicament on the excessive school leadership demands during COVID-19 is to ignore the abundant pre-existing evidence already pointing to this eventuality. However, the way in which Australian school leaders were able to constructively lead during the intensely demanding COVID-19 period does provide additional compelling support for the adoption of a far more relational foundation for leadership theory and practice. Hence, in response to this understanding, this article first presents during-COVID-19 and pre-COVID-19 Australian school leadership research literature to not only describe the evolving concerning issues but also to present the demand for a more relational approach to leadership. Then, the article proceeds to justify and illustrate a new relational approach to the practice of school leadership informed by our theory of organizational ecology. It is proposed that this new way of leading relationally will enable Australian school leaders to ultimately overcome the myriad of complex and stressful crises that now confront them.
Parameters Affecting the Explosion Characteristics of Hybrid Mixtures Arising from the Use of Alternative Energy Sources
Authors: Helegda Matous , Pokorny Jiri , Helegda Iris , Skrinsky Jan , Sinay Juraj
Published: April 2024

Explosions of hybrid mixtures are an interesting theoretical and experimental problem in explosion sciences, because they combine the physicochemical properties of flammable gases and dusts. A hybrid mixture is composed of at least two substances in two or more states. The influence of the common presence of flammable gas on the explosiveness parameters of the combustible dust itself is proven. In this study, we present the effect of higher initiation temperatures, different initial sources of initiation with different energies, and the effect of the volume of explosion chambers on the explosions of hybrid mixtures arising from the use of alternative energy sources. The experiments were carried out in 20 L and 1.00 m3 explosion chambers (according to EN 14034-1+A1:2011–EN 14034-4+A1:2011). The accredited method of the Energy Research Centre, VSB-TU Ostrava, for tests was used. The goal is to approximate the behaviour of these systems under different initiation conditions so that it is possible to avoid excessively conservative or overly optimistic results, which then affect the determination of explosion parameters for practical use. It was found that the volume of the explosion chambers in combination with the used initiation source has a fundamental influence on the course of the explosion characteristics.
Recent Findings on Spawning Patterns and Recommendations for the Fishery Management of the Southern Red Snapper—Lutjanus purpureus (Poey, 1866)—On the Amazon Continental Shelf of Brazil
Authors: da Costa Pinheiro Larissa , Ferreira Beatrice Padovani , Santos Paulo Emílio , Peixoto Ualerson , Bentes Bianca
Published: April 2024

The southern red snapper, Lutjanus purpureus, is one of the primary fishery resources of the Amazon continental shelf. Due to the demand for specimens of less than 900 g in weight, increased fishing effort focusing on this size class has led to a drastic decrease in catches. The present study was based on the analysis of cellular structures in the female gonads (formation of oocytes and other reproductive tissues) to identify reproductive processes and evaluate the effectiveness of current fishery management practices. The presence of postovulatory follicles (POFs) peaked in April, which was interpreted as a period of intense spawning. In addition to POFs, hydrated oocytes peaked in July, indicating either reproductive continuity or a second peak, based on the presence of atretic oocytes and atresia. Given that the local snapper fishery preferentially targets very young individuals, an extension of the closed season from March to July would be recommendable to guarantee the reproductive potential of the spawning stock.
Fractal Characteristics and Microstructure of Coal with Impact of Starch-Polymerized Aluminum Sulfate Fracturing Fluids
Authors: Cai Feng , Zhang Qian , Yang Lingling
Published: April 2024

The degree of irregularity and complexity of the pore structure are comprehensively reflected in the fractal dimension. The porosity of coal was determined by its fractal dimension, where a larger dimension indicates a lower porosity. Fractal theory and the Frenkel–Halsey–Hill (FHH) model were applied to explore the variation rules of concentration on functional groups and pore structure in this study. Combined with infrared spectroscopy (FTIR) and low-temperature nitrogen adsorption, a starch-polymerized aluminum sulfate composite fracturing fluid was prepared, which plays an important role in methane adsorption and permeability of coal samples. The test results showed that, compared with the original coal, the pore volume and specific surface area of each group of coal samples were reduced, the average pore diameter was initially enlarged and then declined, and fractal dimension D1 dropped by 5.4% to 15.4%, while fractal dimension D2 gained 1.2% to 7.9%. Moreover, the nitrogen adsorption of each group of coal samples was obviously lower than the original coal, and the concentration of starch-polymerized aluminum sulfate solution existed at a critical optimal concentration for the modification of the coal samples, and the nitrogen adsorption reached a minimum value of 0.6814 cm3/g at a concentration of 10%. The novel composite solution prepared by the combination of starch and flocculant in this paper enhanced the permeability of the coal seam, which is of great significance in improving the efficiency of coalbed methane mining.
Coated Microneedle System for Delivery of Clotrimazole in Deep-Skin Mycoses
Authors: Jadach Barbara , Nowak Agata , Długaszewska Jolanta , Kordyl Oliwia , Budnik Irena , Osmałek Tomasz
Published: April 2024

Mycoses of the skin are infectious diseases caused by fungal microorganisms that are generally treated with topical agents. However, such therapy is often ineffective and has to be supported by oral use of active substances, which, in turn, can cause many side effects. A good alternative for the treatment of deep-skin mycoses seems to be microneedles (MNs). The aim of this research was to fabricate and evaluate the properties of innovative MNs coated with a hydrogel as potential carriers for clotrimazole (CLO) in the treatment of deep fungal skin infections. A 3D printing technique using a photo-curable resin was employed to produce MNs, which were coated with hydrogels using a dip-coating method. Hydrogels were prepared with carbopol EZ-3 Polymer (Lubrizol) in addition to glycerol and triisopropanolamine. Clotrimazole was introduced into the gel as the solution in ethanol or was suspended. In the first step of the investigation, a texture analysis of hydrogels was prepared with a texture analyzer, and the drug release studies were conducted with the use of automatic Franz diffusion cells. Next, the release profiles of CLO for coated MNs were checked. The last part of the investigation was the evaluation of the antifungal activity of the prepared systems, and the inhibition of the growth of Candida albicans was checked with the diffusion and suspended-plate methods. The texture profile analysis (TPA) for the tested hydrogels showed that the addition of ethanol significantly affects the following studied parameters: hardness, adhesiveness and gumminess, causing a decrease in their values. On the other hand, for the gels with suspended CLO, better spreadability was seen compared to gels with dissolved CLO. The presence of the active substance did not significantly affect the values of the tested parameters. In the dissolution study, the results showed that higher amounts of CLO were released for MNs coated with a hydrogel containing dissolved CLO. Also, microbiological tests proved its efficacy against fungal cultures. Qualitative tests carried out using the diffusion method showed that circular zones of inhibition of fungal growth on the plate were obtained, confirming the hypothesis of effectiveness. The suspension-plate technique confirmed the inhibitory effect of applied CLO on the growth of Candida albicans. From the analysis of the data, the MNs coated with CLO dissolved in hydrogel showed better antifungal activity. All received results seem to be helpful in developing further studies for MNs as carriers of antifungal substances.
Analysis of Vine Nutrition and Productivity Based on Statistical Indicators
Authors: Sala Florin , Camen Dorin , Herbei Mihai Valentin , Blidariu Cosmin
Published: April 2024

Different fertilization systems, methods and techniques result in differences in plant nutrition, with variations in the mineral element contents in the leaves and values of physiological, yield, and production quality indices. This results in large datasets that are often relatively difficult to interpret. Starting from these aspects, the present study analyzed data on the state of plant nutrition (N, P, K, Ca, and Mg content in leaves, in % of dry matter; Fe, Mn, Cu, and Zn content in leaves, in mg/kg of dry matter), physiological indices (chlorophyll leaf content—Chl; leaf area—LA), yield (kg/vine, t/ha), and grape berry quality (sugar and acidity content in berries) of the ‘Burgund mare’ grape cultivar in relation to organic, mineral and foliar fertilization. According to a PCA multivariate analysis, four groups of components were obtained: PC1 including five factors, with positive action in the case of K (r = 0.963), P (r = 0.770) and Cu (r = 0.752), and negative action in the case of Mg (r = −0.940) and Mn (r = −0.759); PC2 including three factors, with positive action for Chl (r = 0.938), LA (r = 0.938) and Ca (r = 0.722); PC3 including one factor (N), with negative action (r = −0.916); and PC4 including two factors, with positive action for Fe (r = 0.869) and Zn (r = 0.761). Based on the one-sample t test (parametric) and the Wilcoxon test (non-parametric) and a threshold value of α = 0.05, the differences between the index values for fertilization variants T2–T13 compared with those for the control variant (T1) were verified. Significant differences were recorded in PC1 for P, Mg and Cu; in PC2 for Chl, LA and Ca; and in PC3 for N. Insignificant differences were recorded in PC1 for K and Mn and in PC4 for Fe and Zn. In the case of the productivity elements and quality indices, significant differences were recorded.
A Review on Abrasive Wear of Aluminum Composites: Mechanisms and Influencing Factors
Authors: Valizade Nima , Farhat Zoheir
Published: April 2024

Aluminum matrix composites (AMCs) find extensive use across diverse industries such as automotive, aerospace, marine, and electronics, owing to their remarkable strength-to-weight ratio, corrosion resistance, and mechanical properties. However, their limited wear resistance poses a challenge for applications requiring high tribological performance. Abrasive wear emerges as the predominant form of wear encountered by AMCs in various industrial settings, prompting significant research efforts aimed at enhancing their wear resistance. Over the past decades, extensive research has investigated the influence of various reinforcements on the abrasive wear behavior of AMCs. This paper presents a comprehensive review of the impact of different variables on the wear and tribological response of aluminum composites. This review explores possible wear mechanisms across various tribosystems, providing examples drawn from the analysis of existing literature. Through detailed discussions on the effects of each variable, conclusions are drawn to offer insights into optimizing the wear performance of AMCs.
Research on Sol-Gel Synthesis of Low-Temperature Na2O-B2O3-SiO2 Vitrified Bonds and Preparation of High-Strength Stacked Abrasives Using the Molding and Crushing Method
Authors: Wang Pei , Liang Lingrui , Li Zhihong , Zhu Yumei
Published: April 2024

Currently, the sol-gel technique is employed in the synthesis of high-performance vitrified bonds; however, its application in the fabrication of stacked abrasives has been minimally explored. Furthermore, the methods utilized in the production of abrasive particles for stacked abrasives are technically challenging and incur high costs, which hinders their actual industrial application. Consequently, this study utilizes the sol-gel approach to synthesize a Na2O-B2O3-SiO2 ternary system vitrified bond powder and employs a molding and crushing method, which offers a lower technological barrier and reduced preparation costs, for the production of abrasive particles subsequently fabricating corundum stacked abrasives. Upon setting the binder composition to a molar ratio of n(SiO2):n(B2O3):n(Na2O) = 65:23:12, it was observed that the crystallization within the glass matrix was minimized and the optimal sintering temperature for the synthesized laminate abrasive to be sustained at 820 °C. At the aforementioned temperature, the binder melt is capable of flowing uniformly amongst the abrasive granules, thereby ensuring a robust encapsulation of the particles. The average single particle compressive strength of the prepared corundum stacked abrasive with a grain size of forty mesh can reach the highest of all composition points at 28.56 N and the average single particle compressive strength of the prepared diamond stacked abrasive is 28.14 N.