bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic correlations between suicide attempts and psychiatric and intermediate phenotypes adjusting for mental disorders
Authors: Fujikane Daisuke , Ohi Kazutaka , Kuramitsu Ayumi , Takai Kentaro , Muto Yukimasa , Sugiyama Shunsuke , Shioiri Toshiki
Published: August 2023

Background

Suicide attempts are a moderately heritable trait, and genetic correlations with psychiatric and related intermediate phenotypes have been reported. However, as several mental disorders as well as major depressive disorder (MDD) are strongly associated with suicide attempts, these genetic correlations could be mediated by psychiatric disorders. Here, we investigated genetic correlations of suicide attempts with psychiatric and related intermediate phenotypes, with and without adjusting for mental disorders.

Methods

To investigate the genetic correlations, we utilized large-scale genome-wide association study summary statistics for suicide attempts (with and without adjusting for mental disorders), nine psychiatric disorders, and 15 intermediate phenotypes.

Results

Without adjusting for mental disorders, suicide attempts had significant positive genetic correlations with risks of attention-deficit/hyperactivity disorder, schizophrenia, bipolar disorder, MDD, anxiety disorders and posttraumatic stress disorder; higher risk tolerance; earlier age at first sexual intercourse, at first birth and at menopause; higher parity; lower childhood IQ, educational attainment and cognitive ability; and lower smoking cessation. After adjusting for mental disorders, suicide attempts had significant positive genetic correlations with the risk of MDD; earlier age at first sexual intercourse, at first birth and at menopause; and lower educational attainment. After adjusting for mental disorders, most of the genetic correlations with psychiatric disorders were decreased, while several genetic correlations with intermediate phenotypes were increased.

Conclusions

These findings highlight the importance of considering mental disorders in the analysis of genetic correlations related to suicide attempts and suggest that susceptibility to MDD, reproductive behaviors, and lower educational levels share a genetic basis with suicide attempts after adjusting for mental disorders.
Local Integration of Urban–Rural Social-assistance Programmes in China: What Are the Driving Forces?
Authors: Peng Chenhong , Wang Julia Shu-Huah
Published: August 2023

This study investigates what drives local variations when pursuing urban–rural equity in social welfare provision in China. We examine how internal features, top-down pressure and horizontal competition have shaped local governments’ decisions to adopt a policy that unifies (yitihua) the urban and rural eligibility thresholds of the world's largest means-tested cash transfer programme (dibao). We collected and coded policies that unify urban–ruraldibaothresholds in 336 prefecture-level divisions between 2011 and 2019. Event history analysis showed that internal fiscal constraint – primarily cost concerns – drove local policy adoption; top-down pressure from provincial governments with a high degree of coercive power in policy directives exerted a significant impact; and the horizontal competition's effect was insignificant. Our findings indicate that fiscal arrangements and top-down policy directives from superior governments with higher coercive power are potent tools to accelerate the adoption of a social welfare policy that would otherwise be unappealing for local officials.
Industrial housing clusters in nineteenth-century Lisbon: finding spatial patterns
Authors: Antunes Gonçalo
Published: August 2023

This article aims to analyse housing solutions used in nineteenth-century Lisbon to deal with explosive demographic and urban development. It particularly focuses on two specific types of industrial housing ensembles created in Lisbon called pátios and vilas operárias. The goal of this article is to analyse the spatial distribution of pátios and vilas operárias in Lisbon. Through the potential of geographic information systems, we aim to understand in a spatial-quantitative way the spatial patterns of these kinds of industrial housing ensembles for the most deprived population. To do so, we used spatial modelling and spatial analysis procedures, including simple spatial distribution, mean centre, standard distance, directional distance and density estimation (hotspots). The new contribution of this article lies in the increase of scientific knowledge about these forms of working-class housing – the pátios and vilas operárias – and their spatial implementation in Lisbon in the second half of the nineteenth century.
How Divided Is Britain? Symbolic Boundaries and Social Cohesion in Post-Brexit Britain
Authors: Richards Lindsay , Heath Anthony
Published: August 2023

In post-Brexit and post-devolution Britain, relationships among the four nations appear fragile. This article aims to discover where British citizens draw the symbolic boundaries that define in-group and out-group members between nations—in particular, England, Scotland, and Wales—and within England. Within England, we also examine class divides and the North–South divide. We operationalize symbolic boundaries through a set of new innovative measures administered in an online survey in 2019. Questions ascertain agreement that the various groups “share my values,” are “people I could get on with,” and are “straightforward and honest.” Results of our descriptive analysis suggest that boundaries are blurred between the British and the Welsh but sharper for the Scottish. We also find sharp but asymmetrical boundaries within England, between the working class and the middle class, and between Northerners and Southerners. Regional differences in perceptions of Southerners map closely onto those of how well Westminster looks after regional interests, which suggests that power imbalances reduce social cohesion.
The End of the Ethnic Bloc Vote? Ethnic Minority Leavers After the Brexit Referendum
Authors: Martin Nicole S. , Sobolewska Maria
Published: August 2023

The 2016 referendum on UK membership in the European Union (EU) led to a realignment of voters along their referendum position (Fieldhouse et al. 2021; Sobolewska and Ford 2020). By December 2019, both the Conservative Party and the Labour Party received approximately 80% of their support from voters who had been on “their” side during the referendum (Fieldhouse et al. 2021). Brexit identities were akin to partisanship, with affective polarization and perceptual screening (Sobolewska and Ford 2020; Sorace and Hobolt 2021). Research also traces the Brexit vote to a growing values divide (Evans and Menon 2017), with ethnocentric attitudes found on opposing sides of the referendum (Sobolewska and Ford 2020). The views of ethnic minority voters, as traditional victims of white ethnocentrism, have been seen largely in this debate as the same as the identity of liberal Remainers. However, despite the assumption that minorities naturally would fall on the side opposing white ethnocentrism, the referendum marked the biggest departure from the traditional ethnic minorities bloc vote since 2005, when a substantial minority of Muslim voters deserted Labour over the Iraq War (Curtice, Fisher, and Steed 2005). The Leave side in the 2016 referendum received approximately one third of the votes of ethnic minorities (Martin, Sobolewska, and Begum 2020), despite Leave also drawing a majority of its support from “UKIP-curious” voters (Evans and Mellon 2019) who had voted for or considered supporting the anti-immigrant radical-right United Kingdom Independence Party.
What Happens When Mini-Publics Are Held in a Deeply Divided Place? Evidence from Northern Ireland
Authors: Pow Jamie , Garry John
Published: August 2023

A pessimistic view of the potential of deliberative mini-publics to effectively contribute to democratic decision making on highly contested issues in deeply divided places asserts that (1) deliberative quality would be low due to the bitterness prompted by discussion of divisive issues, and (2) levels of opinion change would be low given the stubbornly enduring nature of political attitudes in divided places. We empirically examined this pessimistic view using a quasi-experiment involving mini-publics on two separate issues in Northern Ireland: (1) the contentious ethno-national question of Northern Ireland’s constitutional status, and (2) the much less contested and non-ethno-national issue of social care. Contrary to the pessimistic view, we find evidence that from the perspective of the participants themselves, deliberative quality was higher in the mini-public on an ethno-national issue. However, in line with the pessimistic view, levels of self-reported opinion change were significantly lower in the ethno-national mini-public. Overall, the findings highlight the potential for carefully designed deliberative mini-publics to address divisive ethno-national issues: they provide a space for participants to engage with such issues in open and respectful discussion—even if the prospects for attitudinal change are more limited.
Symposium on the Contemporary Politics of the United Kingdom: Introduction and Reflections
Authors: Leal David L.
Published: August 2023

Although my evidence is anecdotal and impressionistic, my sense is that political science teaching, research, and careers are less likely to involve UK politics and the Special Relationship between the United States and the United Kingdom.
INVESTIGATING THE “DEBT–MONEY–PRICES” TRIANGLE: IRVING FISHER’S THEORETICAL JOURNEY TOWARD THE 100% MONEY PROPOSAL
Authors: Demeulemeester Samuel
Published: August 2023

This paper aims to show how the 100% money proposal, which Irving Fisher came to support in 1935, connects to the rest of his work on monetary instability—in particular, to his credit cycle analysis of 1911 and his debt-deflation theory of 1932–33. Behind these respective analyses, we identify a common explanatory pattern of monetary fluctuations, the “debt–money–prices” triangle, which we use to show how Fisher’s explanations evolved over time, and how his advocacy of 100% money came as a logical conclusion.
Representations of phonological changes ingoatand /r/ in theCollection of Nineteenth-century Grammars(CNG)
Authors: WIEMANN MARCO
Published: August 2023

This article presents an analysis of vowels in thegoatset and /r/ in theCollection of Nineteenth-century Grammars(CNG) (cf. Anderwald 2016). My central questions concern the extent to which grammarians provide evidence for early diphthongisation ingoatwords and for changes in the distribution of /r/ variants in nineteenth-century prestige accents. I furthermore evaluate how far grammars are suitable as a source for researching historical sound changes. I show that monophthongs are the most frequently proposed variants forgoatand are often referred to as ‘improper diphthongs’. Some diphthongal descriptions exist for words in open syllables, before /l/ and before plosives in words likeknow,soulandboatrespectively. Concerning the distribution of /r/, I show that most grammars continued to propose two sounds, which were almost exclusively described as ‘rough’ or ‘trilled’, and ‘smooth’ or ‘soft’. However, some grammarians also argued for /r/ having only one sound in all positions and complete post-vocalic /r/ absence. Overall, the grammars in the CNG display a considerable amount of what I assume to be copying from scholars such as Walker (1791) and Murray (1795). Thus, I argue that great care is required when attempting to infer phonological changes from nineteenth-century grammars.
Fundamental Fears in Greece (I)
Authors: Makrygiorgos Nikos , Nega Chrysanthi , Karatza Danae
Published: August 2023

Abstract: Introduction: The present study examined the psychometric properties of a Greek adaptation of the Anxiety Sensitivity Index-3 (ASI-3; Taylor et al., 2007 ). Method: We translated the ASI-3 following a forward-backward method and then, in addition to measures of anxiety and depression (DASS-21; Lovibond & Lovibond, 1995 ; Lyrakos et al., 2011 ), we administered it to a nonclinical general population sample ( N = 611) recruited online. Results: Confirmatory factor analysis revealed that a bifactor model with three orthogonal group factors best fit the data, followed by a correlated three-factor model. An examination of the dimensionality of the ASI-3 and the reliability of its dimensions suggested the presence of a reliable, strong AS general factor and comparatively weaker group factors. The ASI-3 appears to measure AS invariantly across gender. We report preliminary evidence for its convergent, discriminant, and divergent validity. Conclusion: The Greek adaption of the ASI-3 revealed adequate psychometric properties. Future studies should explore its criterion-related validity by administering the Greek adaptation of the ASI-3 to clinical samples and explore its relationship to other key constructs of anxiety sensitivity’s nomological network.
Biological Uptake, Water Mass Mixing and Scavenging Limit the Transport of Manganese‐Rich Waters From the East Antarctic Shelf
Authors: Latour P. , van der Merwe P. , Wuttig K. , Corkill M. , Townsend A. T. , Holmes T. M. , Rintoul S. R. , Weldrick C. K. , Strzepek R. F. , Gault‐Ringold M. , Schlitzer R. , Bowie A. R.
Published: August 2023

Manganese (Mn) is an essential element involved in photosynthesis, yet its concentrations in Southern Ocean open waters are very low, arising from biological uptake and limited external inputs. At southern latitudes, waters overlying the Antarctic shelf are expected to have much higher Mn concentrations due to their proximity to external sources. In this study, we investigated the potential export of Mn‐rich Antarctic shelf waters toward depleted open Southern Ocean waters. Our results showed that while high Mn concentrations were observed over the shelf, biological uptake decreased dissolved Mn concentrations in surface waters north of the South Antarctic Circumpolar Current Front (<0.1 nmol kg−1), limiting its export. Conversely, mixing between Mn‐rich Antarctic Bottom Waters and Mn‐depleted Lower Circumpolar Deep Waters combined with oxidative and scavenging processes led to a decrease in dissolved Mn concentrations within bottom waters, with distance from the coast. Particulate Mn concentrations also showed a decreasing trend with distance from the coast. A comparison with other Antarctic coastal regions suggests this bottom water Mn removal may be widespread and that East Antarctica may be characterized by lower Mn concentrations compared to other regions. Still, subsurface dissolved Mn maxima (0.3–0.6 nmol kg−1) represented a potential reservoir for surface waters. We hypothesize that these high subsurface values result from external sources near the shelf. Overall, these results suggest that the moderate lateral export of trace metal‐enriched waters contributes to the extremely low and potentially limiting Mn concentrations previously reported further north in this Southern Ocean region.
Continental Deep Subduction Versus Subduction Cessation: The Fate of Collisional Orogens
Authors: Wang Yang , Li Zhong‐Hai , Huangfu Pengpeng
Published: August 2023

The contrasting fates of collisional orogens, that is, continental deep subduction or subduction cessation, are widely recognized by petrological, paleomagnetic and geophysical observations. However, the mechanisms of such different collisional modes, especially the dynamics of continental deep subduction, are controversial. In this study, we integrate the phase transition‐induced density evolutions into a thermo‐mechanical numerical model. Combing the systematic petrological‐thermo‐mechanical models with force balance analysis, we find that the high metamorphic transformation degree, mildly depleted mantle composition of the subcontinental lithosphere, and a long preceding oceanic slab, increase the driving force for continental deep subduction. Additionally, the rheologically weak continental crust and asthenospheric mantle decrease the resistance force and promote deep subduction. Otherwise, the continental subduction cessation mode is favored. The calculations of slab negative buoyancy indicate that the phase transition‐induced metamorphic densification of the subducted continental crust and the mildly to moderately depleted lithospheric mantle can provide a great slab pull force to sustain the continued continental deep subduction; however, the positive buoyancy of highly depleted Archean lithospheric mantle impedes deep subduction and causes subduction cessation. Based on systematic numerical models, we also evaluate the crustal mass balance or deficit in continental collisional system, which indicates that ∼12%–47% of pre‐collisional felsic crust could be subducted deeply with the sinking slab in the regime of continental deep subduction. In contrast, the recycled felsic crust is negligible in the regime of subduction cessation. Thus, the different modes of continental collision play a crucial role in the global crustal recycling and related mantle heterogeneities.
Environmental Controls on MCS Lifetime Rainfall Over Tropical Oceans
Authors: Chen Xingchao , Leung L. Ruby , Feng Zhe , Yang Qiu
Published: August 2023

Mesoscale convective systems (MCSs) contribute a majority of rainfall over tropical oceans. However, our understanding of the environmental controls on tropical oceanic MCS precipitation remains incomplete. Using 20‐year of satellite observations, reanalysis data, and MCS tracking, we found that MCSs initiating in a mesoscale environment with enhanced lower‐free‐tropospheric moisture, warmer middle troposphere, stronger low‐level ascent, and stronger deep‐layer (surface‐400 hPa) wind shear tend to produce more precipitation during their lifetimes. While most of these environmental factors are correlated with one another, the deep‐layer shear is not. A rapid pickup in MCS lifetime rainfall is found when the lower‐free‐tropospheric specific humidity exceeds 10 g kg−1. This nonlinearity is mostly dominated by the nonlinear increase in MCS area. On the other hand, both MCS area and rain rate increase quasi‐linearly with the deep‐layer shear. The increase in rain rate is related to the enhancement of heavy precipitating convective activity with deep‐layer shear.
Inhibition of Toll-like receptor 4 and Interleukin-1 receptor prevent SARS-CoV-2 mediated kidney injury
Authors: Nakazawa Daigo , Takeda Yohei , Kanda Masatoshi , Tomaru Utano , Ogawa Haruko , Kudo Takashi , Shiratori-Aso Satoka , Watanabe-Kusunoki Kanako , Ueda Yusho , Miyoshi Atsuko , Hattanda Fumihiko , Nishio Saori , Uozumi Ryo , Ishizu Akihiro , Atsumi Tatsuya
Published: August 2023

Acute kidney injury (AKI) is a common and severe complication of the coronavirus disease 2019 (COVID-19). Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) directly affects the glomerular and tubular epithelial cells to induce AKI; however, its pathophysiology remains unclear. Here, we explored the underlying mechanisms and therapeutic targets of renal involvement in COVID-19. We developed an in vitro human kidney cellular model, including immortalized tubular epithelial and endothelial cell lines, demonstrating that SARS-CoV-2 directly triggers cell death. To identify the molecular targets in the process of SARS-CoV-2-mediated cell injury, we performed transcriptional analysis using RNA sequencing. Tubular epithelial cells were more prone to dying by SARS-CoV-2 than endothelial cells; however, SARS-CoV-2 did not replicate in renal cells, distinct from VeroE6/transmembrane protease serine 2 cells. Transcriptomic analysis revealed increased inflammatory and immune-related gene expression levels in renal cells incubated with SARS-CoV-2. Toll-like receptor (TLR) 3 in renal cells recognized viral RNA and underwent cell death. Furthermore, analysis of upstream regulators identified several key transcriptional regulators. Among them, inhibition of the interleukin-1 receptor (IL-1R) and TLR4 pathways protects tubular epithelial and endothelial cells from injury via regulation of the signal transducer and activator of transcription protein-3/nuclear factor-kB pathway. Our results reveal that SARS-CoV-2 directly injures renal cells via the proinflammatory response without viral replication, and that IL-1R and TLR4 may be used as therapeutic targets for SARS-CoV-2 mediated kidney injury.
Characterization of circSEC11A as a novel regulator of Iodine-125 radioactive seed-induced anticancer effects in hepatocellular carcinoma via targeting ZHX2/GADD34 axis
Authors: Li Dong , Wang Wujie , Liu Bin , Jin Die , Wang Yang , He Guanghui , Guo Lei , Liu Wen , Li Yuliang
Published: August 2023

Iodine-125 (I-125) radioactive seed implantation is used for the local treatment of hepatocellular carcinoma (HCC), but the molecular mechanisms regulating its anticancer effects remain incompletely understood. In this study, we report that hsa_circ_0000647 (circSEC11A) is highly expressed after I-125 treatment in HCC cell lines and tissues and is a key regulator of I-125-induced anticancer effects. CircSEC11A acts as a competing endogenous RNA (ceRNA) to sponge miR-3529-3p, promoting the expression of zinc fingers and homeoboxes 2 (ZHX2) and enhancing I-125-induced anticancer effects. Dual-luciferase reporter assay, RNA pull-down, RNA immunoprecipitation, and fluorescence in situ hybridization were thereafter performed to verify the interaction among the molecules. Anticancer effects were detected using CCK-8, flow cytometry, TUNEL, EdU, transwell, and wound healing assays. Furthermore, ZHX2 transcriptionally inhibits GADD34, a negative regulator of endoplasmic reticulum stress (ERS), to enhance I-125- induced anticancer effects in vivo and in vitro. In conclusion, we characterized circSEC11A as a novel regulator of I-125-induced anticancer effects in HCC via miR-3529-3p/ZHX2/GADD34 axis-mediated ERS. Thus, circSEC11A may act as a potential therapeutic target for I-125 implantation in the clinic.
Full-color persistent room temperature phosphorescent elastomers with robust optical properties
Authors: Wei Juan , Zhu Mingye , Du Tingchen , Li Jangang , Dai Peiling , Liu Chenyuan , Duan Jiayu , Liu Shujuan , Zhou Xingcheng , Zhang Sudi , Guo Luo , Wang Hao , Ma Yun , Huang Wei , Zhao Qiang
Published: August 2023

Persistent room temperature phosphorescent materials with unique mechanical properties and robust optical properties have great potential in flexible electronics and photonics. However, developing such materials remains a formidable challenge. Here, we present highly stretchable, lightweight, and multicolored persistent luminescence elastomers, produced by incorporating ionic room temperature phosphorescent polymers and polyvinyl alcohol into a polydimethylsiloxane matrix. These prepared elastomers exhibit high optical transparency in daylight and emit bright persistent luminescence after the removal of 365 nm excitation. The homogeneous distribution of polymers within the matrix has been confirmed by confocal fluorescence microscopy, scanning electron microscopy, and atomic force microscopy. Mechanical property investigations revealed that the prepared persistent luminescence elastomers possess satisfactory stretchability. Impressively, these elastomers maintain robust optical properties even under extensive and repeated mechanical deformations, a characteristic previously unprecedented. These fantastic features make these persistent luminescence elastomers ideal candidates for potential applications in wearable devices, flexible displays, and anti-counterfeiting.
Complementarity-determining region clustering may cause CAR-T cell dysfunction
Authors: Sarén Tina , Saronio Giulia , Marti Torrell Paula , Zhu Xu , Thelander Josefin , Andersson Yasmin , Hofström Camilla , Nestor Marika , Dimberg Anna , Persson Helena , Ramachandran Mohanraj , Yu Di , Essand Magnus
Published: August 2023

Chimeric antigen receptor (CAR)-T cell therapy is rapidly advancing as cancer treatment, however, designing an optimal CAR remains challenging. A single-chain variable fragment (scFv) is generally used as CAR targeting moiety, wherein the complementarity-determining regions (CDRs) define its specificity. We report here that the CDR loops can cause CAR clustering, leading to antigen-independent tonic signalling and subsequent CAR-T cell dysfunction. We show via CARs incorporating scFvs with identical framework and varying CDR sequences that CARs may cluster on the T cell surface, which leads to antigen-independent CAR-T cell activation, characterized by increased cell size and interferon (IFN)-γ secretion. This results in CAR-T cell exhaustion, activation-induced cell death and reduced responsiveness to target-antigen-expressing tumour cells. CDR mutagenesis confirms that the CAR-clustering is mediated by CDR-loops. In summary, antigen-independent tonic signalling can be induced by CDR-mediated CAR clustering, which could not be predicted from the scFv sequences, but could be tested for by evaluating the activity of unstimulated CAR-T cells.
High-energy photoemission final states beyond the free-electron approximation
Authors: Strocov V. N. , Lev L. L. , Alarab F. , Constantinou P. , Wang X. , Schmitt T. , Stock T. J. Z. , Nicolaï L. , Očenášek J. , Minár J.
Published: August 2023

Three-dimensional (3D) electronic band structure is fundamental for understanding a vast diversity of physical phenomena in solid-state systems, including topological phases, interlayer interactions in van der Waals materials, dimensionality-driven phase transitions, etc. Interpretation of ARPES data in terms of 3D electron dispersions is commonly based on the free-electron approximation for the photoemission final states. Our soft-X-ray ARPES data on Ag metal reveals, however, that even at high excitation energies the final states can be a way more complex, incorporating several Bloch waves with different out-of-plane momenta. Such multiband final states manifest themselves as a complex structure and added broadening of the spectral peaks from 3D electron states. We analyse the origins of this phenomenon, and trace it to other materials such as Si and GaN. Our findings are essential for accurate determination of the 3D band structure over a wide range of materials and excitation energies in the ARPES experiment.
Cell-type-specific co-expression inference from single cell RNA-sequencing data
Authors: Su Chang , Xu Zichun , Shan Xinning , Cai Biao , Zhao Hongyu , Zhang Jingfei
Published: August 2023

The advancement of single cell RNA-sequencing (scRNA-seq) technology has enabled the direct inference of co-expressions in specific cell types, facilitating our understanding of cell-type-specific biological functions. For this task, the high sequencing depth variations and measurement errors in scRNA-seq data present two significant challenges, and they have not been adequately addressed by existing methods. We propose a statistical approach, CS-CORE, for estimating and testing cell-type-specific co-expressions, that explicitly models sequencing depth variations and measurement errors in scRNA-seq data. Systematic evaluations show that most existing methods suffered from inflated false positives as well as biased co-expression estimates and clustering analysis, whereas CS-CORE gave accurate estimates in these experiments. When applied to scRNA-seq data from postmortem brain samples from Alzheimer’s disease patients/controls and blood samples from COVID-19 patients/controls, CS-CORE identified cell-type-specific co-expressions and differential co-expressions that were more reproducible and/or more enriched for relevant biological pathways than those inferred from existing methods.
How climate policy commitments influence energy systems and the economies of US states
Authors: Bergquist Parrish , Warshaw Christopher
Published: August 2023

In the United States, state governments have been the locus of action for addressing climate change. However, the lack of a holistic measure of state climate policy has prevented a comprehensive assessment of state policies’ effectiveness. Here, we assemble information from 25 individual policies to develop an aggregate index of state climate policies from 2000-2020. The climate policy index highlights variation between states which is difficult to assess in single policy studies. Next, we examine the environmental and economic consequences of state climate policy. A standard-deviation increase in climate policy is associated with a 5% reduction in per-capita electricity-sector CO2 emissions and a 2% reduction in economy-wide CO2 emissions per capita. We do not find evidence that more stringent climate policy harms states’ economies. Our results make clear the benefits of state climate policy, while showing that current state efforts are unlikelyto meet the US goal under the Paris Climate Accord.
WDR77 inhibits prion-like aggregation of MAVS to limit antiviral innate immune response
Authors: Li Jiaxin , Zhang Rui , Wang Changwan , Zhu Junyan , Ren Miao , Jiang Yingbo , Hou Xianteng , Du Yangting , Wu Qing , Qi Shishi , Li Lin , Chen She , Yang Hui , Hou Fajian
Published: August 2023

RIG-I-MAVS signaling pathway plays a crucial role in defending against pathogen infection and maintaining immune balance. Upon detecting viral RNA, RIG-I triggers the formation of prion-like aggregates of the adaptor protein MAVS, which then activates the innate antiviral immune response. However, the mechanisms that regulate the aggregation of MAVS are not yet fully understood. Here, we identified WDR77 as a MAVS-associated protein, which negatively regulates MAVS aggregation. WDR77 binds to MAVS proline-rich region through its WD2-WD3-WD4 domain and inhibits the formation of prion-like filament of recombinant MAVS in vitro. In response to virus infection, WDR77 is recruited to MAVS to prevent the formation of its prion-like aggregates and thus downregulate RIG-I-MAVS signaling in cells. WDR77 deficiency significantly potentiates the induction of antiviral genes upon negative-strand RNA virus infections, and myeloid-specific Wdr77-deficient mice are more resistant to RNA virus infection. Our findings reveal that WDR77 acts as a negative regulator of the RIG-I-MAVS signaling pathway by inhibiting the prion-like aggregation of MAVS to prevent harmful inflammation.
Long operating lifetime mid-infrared LEDs based on black phosphorus
Authors: Higashitarumizu Naoki , Tajima Shogo , Kim Jongchan , Cai Mingyang , Javey Ali
Published: August 2023

Black phosphorus (BP) is a narrow bandgap layered semiconductor promising for mid-infrared optoelectronic applications. BP-based devices have been shown to surpass state-of-the-art mid-infrared detectors and light-emitting diodes (LEDs) in terms of performance. Despite their device advantages, the material’s inherent instability in the air could hinder its use in practical optoelectronic applications. Here, we investigated the impact of passivation on the device lifetime of BP LEDs, which deteriorate in a matter of seconds without using passivation. The lifetime is significantly extended with an Al2O3 passivation layer and nitrogen packaging via atomic layer deposition and ultra-violet curable resin sealing. The operational lifetime (half-life) at room temperature is extrapolated to be ~15,000 h with an initial power density of 340 mW/cm2 based on accelerated life testing. The present results indicate that efficient BP optoelectronics can be highly robust through simple and scalable packaging technologies, with important practical implications for mid-infrared applications.
A multi-omic Nicotiana benthamiana resource for fundamental research and biotechnology
Authors: Ranawaka Buddhini , An Jiyuan , Lorenc Michał T. , Jung Hyungtaek , Sulli Maria , Aprea Giuseppe , Roden Sally , Llaca Victor , Hayashi Satomi , Asadyar Leila , LeBlanc Zacharie , Ahmed Zuba , Naim Fatima , de Campos Samanta Bolzan , Cooper Tal , de Felippes Felipe F. , Dong Pengfei , Zhong Silin , Garcia-Carpintero Victor , Orzaez Diego , Dudley Kevin J. , Bombarely Aureliano , Bally Julia , Winefield Christopher , Giuliano Giovanni , Waterhouse Peter M.
Published: August 2023

Nicotiana benthamiana is an invaluable model plant and biotechnology platform with a ~3 Gb allotetraploid genome. To further improve its usefulness and versatility, we have produced high-quality chromosome-level genome assemblies, coupled with transcriptome, epigenome, microRNA and transposable element datasets, for the ubiquitously used LAB strain and a related wild accession, QLD. In addition, single nucleotide polymorphism maps have been produced for a further two laboratory strains and four wild accessions. Despite the loss of five chromosomes from the ancestral tetraploid, expansion of intergenic regions, widespread segmental allopolyploidy, advanced diploidization and evidence of recent bursts of Copia pseudovirus (Copia) mobility not seen in other Nicotiana genomes, the two subgenomes of N. benthamiana show large regions of synteny across the Solanaceae. LAB and QLD have many genetic, metabolic and phenotypic differences, including disparate RNA interference responses, but are highly interfertile and amenable to genome editing and both transient and stable transformation. The LAB/QLD combination has the potential to be as useful as the Columbia-0/Landsberg errecta partnership, utilized from the early pioneering days of Arabidopsis genomics to today.
Rapid mapping of alloy surface phase diagrams via Bayesian evolutionary multitasking
Authors: Han Shuang , Lysgaard Steen , Vegge Tejs , Hansen Heine Anton
Published: August 2023

Surface phase diagrams (SPDs) are essential for understanding the dependence of surface chemistry on reaction condition. For multi-component systems such as metal alloys, the derivation of such diagrams often relies on separate first-principles global optimization tasks under different reaction conditions. Here we show that this can be significantly accelerated by leveraging the fact that all tasks essentially share a unified configurational search space, and only a single expensive electronic structure calculation is required to evaluate the stabilities of a surface structure under all considered reaction conditions. As a general solution, we propose a Bayesian evolutionary multitasking (BEM) framework combining Bayesian statistics with evolutionary multitasking, which allows efficient mapping of SPDs even for very complex surface systems. As proofs of concept, we showcase the performance of our methods in deriving the alloy SPDs for two heterogeneous catalytic systems: the electrochemical oxygen reduction reaction (ORR) and the gas phase steam methane reforming (SMR) reaction.
The γ/γ′ microstructure in CoNiAlCr-based superalloys using triple-objective optimization
Authors: Liu Pei , Huang Haiyou , Wen Cheng , Lookman Turab , Su Yanjing
Published: August 2023

Optimizing several properties simultaneously based on small data-driven machine learning in complex black-box scenarios can present difficulties and challenges. Here we employ a triple-objective optimization algorithm deduced from probability density functions of multivariate Gaussian distributions to optimize the γ′ volume fraction, size, and morphology in CoNiAlCr-based superalloys. The effectiveness of the algorithm is demonstrated by synthesizing alloys with desired γ/γ′ microstructure and optimizing γ′ microstructural parameters. In addition, the method leads to incorporating refractory elements to improve γ/γ′ microstructure in superalloys. After four iterations of experiments guided by the algorithm, we synthesize sixteen alloys of relatively high creep strength from ~120,000 candidates of which three possess high γ′ volume fraction (>54%), small γ′ size (<480 nm), and high cuboidal γ′ fraction (>77%).
Effect of surface modification on interfacial behavior in bioabsorbable magnesium wire reinforced poly-lactic acid polymer composites
Authors: Ali Wahaaj , Echeverry-Rendón Mónica , Kopp Alexander , González Carlos , LLorca Javier
Published: August 2023

The mechanical behavior, corrosion mechanisms, and cytocompatibility at the interface of magnesium wires reinforced poly-lactic acid polymer composites were studied by in vitro degradation study of 180 days. Surface modification of Mg wires by plasma-electrolytic oxidation improved the interface shear strength from 10.9 MPa to 26.3 MPa which decreased to 8 MPa and 13.6 MPa in Mg/PLA and PEO-Mg/PLA composites, respectively, after 42 days of in vitro degradation. Cross-sections of the composite showed good cytocompatibility, although the cells tended to migrate towards the PLA regions and avoided the surface of the Mg wires. Corrosion of Mg wires (without surface modification) was very fast in composite while corrosion of surface-modified Mg wires was significantly reduced, hydrogen gas was suppressed and only 3% mass loss of Mg wires was found after 180 days. Finally, the corrosion mechanisms at interface were discussed for both composites.
Protective antibody threshold of RTS,S/AS01 malaria vaccine correlates antigen and adjuvant dose in mouse model
Authors: Genito Christopher J. , Brooks Katherine , Smith Alexis , Ryan Emma , Soto Kim , Li Yuanzhang , Warter Lucile , Dutta Sheetij
Published: August 2023

Mouse models are useful for the early down-selection of malaria vaccine candidates. The Walter Reed Army Institute of Research has optimized a transgenic Plasmodium berghei sporozoite challenge model to compare the efficacy of Plasmodium falciparum circumsporozoite protein (CSP) vaccines. GSK’s RTS,S vaccine formulated in the adjuvant AS01 can protect malaria-naïve individuals against malaria. We report that the RTS,S/AS01 vaccine induces high level sterile protection in our mouse model. Down titration of the antigen at a constant AS01 dose revealed a potent antigen dose-sparing effect and the superiority of RTS,S/AS01 over a soluble CSP antigen. RTS,S-mediated protective immunity was associated with a threshold of major repeat antibody titer. Combined titration of the antigen and adjuvant showed that reducing the adjuvant could improve antibody boosting post-3rd vaccination and reduce the threshold antibody concentration required for protection. Mouse models can provide a pathway for preclinical assessment of strategies to improve CSP vaccines against malaria.
Multiple loss-of-function mutations of carotenoid cleavage dioxygenase 4 reveal its major role in both carotenoid level and apocarotenoid composition in flue-cured mature tobacco leaves
Authors: Magome Hiroshi , Arai Masao , Oyama Kiyoshi , Nishiguchi Ryo , Takakura Yoshimitsu
Published: August 2023

Apocarotenoid volatiles contribute to the flavor of many agricultural products. In many flowering plants, carotenoid cleavage dioxygenase 4 (CCD4) is involved in the decomposition of carotenoids and resultant production of C13-apocarotenoids, such as β-ionone derived from β-carotene. To understand the possible role of tobacco CCD4 genes (NtCCD4-S, NtCCD4-T1 and NtCCD4-T2) in these processes, we analyzed loss-of-function phenotypes. RNA interference transgenic plants showed yellow color in mature (senescent) leaves. Mature leaves of chemically induced double-mutant plants showed a stronger yellow color, and those of triple-mutant plants showed a pronounced yellow color. Carotenoid analysis of the leaves from mutants showed that lutein and β-carotene increased in line with the degree of color change compared to wild type, whereas there was little change in green color in their young leaves. This result indicates that CCD4s are important for the decomposition of carotenoids in the tobacco leaf maturation process. Analysis of apocarotenoids in flue-cured leaves of the multiple-mutant plants showed that many compounds, including megastigmatrienones, were decreased in comparison to wild type, whereas intriguingly β-ionone and dihydroactinidiolide were increased. Our results suggest that CCD4s play a key role in both carotenoid level and apocarotenoid composition in flue-cured mature tobacco leaves.
Gender differences in footwear characteristics between half and full marathons in China: a cross-sectional survey
Authors: Xia Yuyu , Shen Siqin , Jia Sheng-Wei , Teng Jin , Gu Yaodong , Fekete Gusztáv , Korim Tamás , Zhao Haotian , Wei Qiang , Yang Fan
Published: August 2023

There are concerns about the risk of injuries caused by marathons in China. Since male and female runners have different injury risks, gender differences in running shoe functionality should be further complemented. A supervised questionnaire survey of 626 marathon runners was collected. The questionnaire was categorized into four sections: (1) participant profile, (2) importance of shoe properties, (3) functional evaluation of shoe properties and (4) importance ranking of shoe properties. The Mann–Whitney U test, Fisher’s exact test of cross tabulation and Chi-square test, and two-way ANOVA were used to analyze the results of this survey. The significance level was set at P < 0.05. The full marathon participants were older than the half marathon participants. There was no gender difference in the importance of shoe features to elite runners. In addition, women are more concerned about upper elasticity and have higher requirements for running shoes than men. Women were more focused on injury prevention, while men were more focused on running performance. Heel cushioning was identified by all participants as the most important running shoe feature. There were no gender differences between elite players’ demand for running shoes, but significant gender differences were found between genders at other running levels.
Assessing individual risk and the latent transmission of COVID-19 in a population with an interaction-driven temporal model
Authors: Marmor Yanir , Abbey Alex , Shahar Yuval , Mokryn Osnat
Published: August 2023

Interaction-driven modeling of diseases over real-world contact data has been shown to promote the understanding of the spread of diseases in communities. This temporal modeling follows the path-preserving order and timing of the contacts, which are essential for accurate modeling. Yet, other important aspects were overlooked. Various airborne pathogens differ in the duration of exposure needed for infection. Also, from the individual perspective, Covid-19 progression differs between individuals, and its severity is statistically correlated with age. Here, we enrich an interaction-driven model of Covid-19 and similar airborne viral diseases with (a) meetings duration and (b) personal disease progression. The enriched model enables predicting outcomes at both the population and the individual levels. It further allows predicting individual risk of engaging in social interactions as a function of the virus characteristics and its prevalence in the population. We further showed that the enigmatic nature of asymptomatic transmission stems from the latent effect of the network density on this transmission and that asymptomatic transmission has a substantial impact only in sparse communities.
Machine learning revealed symbolism, emotionality, and imaginativeness as primary predictors of creativity evaluations of western art paintings
Authors: Spee Blanca T. M. , Mikuni Jan , Leder Helmut , Scharnowski Frank , Pelowski Matthew , Steyrl David
Published: August 2023

Creativity is a compelling yet elusive phenomenon, especially when manifested in visual art, where its evaluation is often a subjective and complex process. Understanding how individuals judge creativity in visual art is a particularly intriguing question. Conventional linear approaches often fail to capture the intricate nature of human behavior underlying such judgments. Therefore, in this study, we employed interpretable machine learning to probe complex associations between 17 subjective art-attributes and creativity judgments across a diverse range of artworks. A cohort of 78 non-art expert participants assessed 54 artworks varying in styles and motifs. The applied Random Forests regressor models accounted for 30% of the variability in creativity judgments given our set of art-attributes. Our analyses revealed symbolism, emotionality, and imaginativeness as the primary attributes influencing creativity judgments. Abstractness, valence, and complexity also had an impact, albeit to a lesser degree. Notably, we observed non-linearity in the relationship between art-attribute scores and creativity judgments, indicating that changes in art-attributes did not consistently correspond to changes in creativity judgments. Employing statistical learning, this investigation presents the first attribute-integrating quantitative model of factors that contribute to creativity judgments in visual art among novice raters. Our research represents a significant stride forward building the groundwork for first causal models for future investigations in art and creativity research and offering implications for diverse practical applications. Beyond enhancing comprehension of the intricate interplay and specificity of attributes used in evaluating creativity, this work introduces machine learning as an innovative approach in the field of subjective judgment.
Impact of different sowing dates and irrigation levels on NPK absorption, yield and water use efficiency of maize
Authors: Abaza Ahmed S. D. , Elshamly Ayman M. S. , Alwahibi Mona S. , Elshikh Mohamed S. , Ditta Allah
Published: August 2023

Upper Egypt experiences high temperatures during summer and low temperatures during winter, which significantly impacts the sowing dates of maize in this region. The productivity of maize crops and water use efficiency can be greatly affected by water stress and sowing dates (SDs). Therefore, it is crucial to determine the optimal irrigation level and SDs based on local conditions. To assess the effects, two irrigation levels were employed: (1) control (full irrigation water applied) and (2) 70% of irrigation water. Field experiments were conducted at the National Water Research Center's water studies and research complex station in Toshka. The aim was to evaluate two irrigation levels (full and limited irrigation) across five SDs (early: mid-February and March, normal: mid-June, and late: mid-August and September) in both 2019 and 2020, in order to identify the ideal sowing date (SD) and irrigation level. The normal SD resulted in an increased the growth season length between plant emergence and maturity. Conversely, the late SD reduced the number of days until plant maturity, resulting in higher grain yields and water use efficiency (WUE). Notably, the SD in September, coupled with the 70% irrigation level, yielded the highest productivity and WUE, with a productivity of 7014 kg ha−1 and a WUE of 0. 9 kg m−3. Based on the findings, it is recommended that regions with similar conditions consider cultivating maize seeds in September, adopting a 70% irrigation level, to achieve optimal N uptake, growth traits (plant height, ear length, ear weight, number of rows per ear, and grain index weight), yield, and WUE.