bluuebunny/crossref_metadata_2025 huggingface dataset:
Evolution and developmental functions of the dystrophin-associated protein complex: beyond the idea of a muscle-specific cell adhesion complex
Authors: Mirouse Vincent
Published: June 2023

The Dystrophin-Associated Protein Complex (DAPC) is a well-defined and evolutionarily conserved complex in animals. DAPC interacts with the F-actin cytoskeleton via dystrophin, and with the extracellular matrix via the membrane protein dystroglycan. Probably for historical reasons that have linked its discovery to muscular dystrophies, DAPC function is often described as limited to muscle integrity maintenance by providing mechanical robustness, which implies strong cell-extracellular matrix adhesion properties. In this review, phylogenetic and functional data from different vertebrate and invertebrate models will be analyzed and compared to explore the molecular and cellular functions of DAPC, with a specific focus on dystrophin. These data reveals that the evolution paths of DAPC and muscle cells are not intrinsically linked and that many features of dystrophin protein domains have not been identified yet. DAPC adhesive properties also are discussed by reviewing the available evidence of common key features of adhesion complexes, such as complex clustering, force transmission, mechanosensitivity and mechanotransduction. Finally, the review highlights DAPC developmental roles in tissue morphogenesis and basement membrane (BM) assembly that may indicate adhesion-independent functions.
Potential alternative drug treatment for bone giant cell tumor
Authors: Chen Zhangxin , Zhang Cong , Hong Haisen , Xu Wenbin , Sha Mo , Ding Zhenqi
Published: June 2023

Background: Bone giant cell tumor (BGCT) is one of the world’s major disease types of locally aggressive bone tumors. In recent years, denosumab treatment has been introduced before curettage surgery. However, the current therapeutic was practical only sometimes, given the local recurrence effects after discontinuation of denosumab. Due to the complex nature of BGCT, this study aims to use bioinformatics to identify potential genes and drugs associated with BGCT.

Methods: The genes that integrate BGCT and fracture healing were determined by text mining. The gene was obtained from the pubmed2ensembl website. We filtered out common genes for the function, and signal pathway enrichment analyses were implemented. The protein–protein interaction (PPI) networks and the hub genes were screened by MCODE built-in Cytoscape software. Lastly, the confirmed genes were queried in the Drug Gene Interaction Database to determine potential genes and drugs.

Results: Our study finally identified 123 common specific genes in bone giant cell tumors and fracture healing text mining concepts. The GO enrichment analysis finally analyzed 115 characteristic genes in BP, CC, and MF. We selected 10 KEGG pathways and identified 68 characteristic genes. We performed protein–protein interaction analysis (PPI) on 68 selected genes and finally identified seven central genes. In this study, these seven genes were substituted into drug–gene interactions, and there were 15 antineoplastic drugs, 1 anti-involving drug, and 1 anti-influenza drug.

Conclusion: The 7 genes (including ANGPT2, COL1A1, COL1A2, CTSK, FGFR1, NTRK2, and PDGFB) and 17 drugs, which have not been used in BGCT, but 6 of them approved by the FDA for other diseases, could be potential genes and drugs, respectively, to improve BGCT treatment. In addition, the correlation study and analysis of potential drugs through genes provide great opportunities to promote the repositioning of drugs and the study of pharmacology in the pharmaceutical industry.
Decision making for transformative change: exploring model use, structural uncertainty and deep leverage points for change in decision making under deep uncertainty
Authors: Few Sheridan , Bonjean Stanton Muriel C. , Roelich Katy
Published: June 2023

Moving to a low carbon society requires pro-active decisions to transform social and physical systems and their supporting infrastructure. However, the inherent complexity of these systems leads to uncertainty in their responses to interventions, and their critical societal role means that stakes are high. Techniques for decision making under deep uncertainty (DMDU) have recently begun to be applied in the context of transformation to a low carbon society. Applying DMDU to support transformation necessitates careful attention to uncertainty in system relationships (structural uncertainty), and to actions targeting deep leverage points to transform system relationships. This paper presents outcomes of a structured literature review of 44 case studies in which DMDU is applied to infrastructure decisions. Around half of these studies are found to neglect structural uncertainty entirely, and no study explicitly considers alternative system conceptions. Three quarters of studies consider actions targeting only parameters, a shallow leverage point for system transformation. Where actions targeting deeper leverage points are included, models of system relationships are unable to represent the transformative change these interventions could effect. The lack of attention to structural uncertainty in these studies could lead to misleading results in complex and poorly understood systems. The lack of interventions targeting deep leverage points could lead to neglect of some of the most effective routes to achieving transformative change. This review recommends greater attention to deeper leverage points and structural uncertainty in applications of DMDU targeting transformative change.
Variable trust in public health messaging during the first year of the COVID-19 pandemic in Southeast Alaska
Authors: van Doren Taylor P. , Brown Ryan A. , Izenberg Max , Heintz Ron , Simmons Callie , Paddock Raymond
Published: June 2023

Public health messaging in the United States during the COVID-19 pandemic has been variable in effectiveness. Different levels of governmental institutions have different goals and methods; it is unclear how messaging from these disparate levels is received, trusted, and implemented. We investigate the degrees of trust of Alaska Native and non-Alaska Native people in Southeast Alaska for the U.S. federal, Alaska state, and local Southeast Alaskan governments to parse how Southeast Alaskans feel about relative preparation, actions, and public health messaging. We use data collected in two waves of a regional survey: the first in April-June 2020, and the second in November 2020-February 2021. Results indicate that trust in the federal government was significantly lower than in the Alaska state government for each time period, and trust in both federal and state government significantly decreased between the two periods. Trust in the local governments of Southeast Alaska were significantly higher than both state and federal levels, and increased between the two survey waves, albeit insignificantly. We discuss potential drivers of these observations and outline how this can be leveraged for more comprehensive research into how relatively small communities with a large Native presence perceive public health messaging from different sources.
Identification of risk factors for infection after mitral valve surgery through machine learning approaches
Authors: Zhang Ningjie , Fan Kexin , Ji Hongwen , Ma Xianjun , Wu Jingyi , Huang Yuanshuai , Wang Xinhua , Gui Rong , Chen Bingyu , Zhang Hui , Zhang Zugui , Zhang Xiufeng , Gong Zheng , Wang Yongjun
Published: June 2023

Background

Selecting features related to postoperative infection following cardiac surgery was highly valuable for effective intervention. We used machine learning methods to identify critical perioperative infection-related variables after mitral valve surgery and construct a prediction model.

Methods

Participants comprised 1223 patients who underwent cardiac valvular surgery at eight large centers in China. The ninety-one demographic and perioperative parameters were collected. Random forest (RF) and least absolute shrinkage and selection operator (LASSO) techniques were used to identify postoperative infection-related variables; the Venn diagram determined overlapping variables. The following ML methods: random forest (RF), extreme gradient boosting (XGBoost), Support Vector Machine (SVM), Gradient Boosting Decision Tree (GBDT), AdaBoost, Naive Bayesian (NB), Logistic Regression (LogicR), Neural Networks (nnet) and artificial neural network (ANN) were developed to construct the models. We constructed receiver operating characteristic (ROC) curves and the area under the ROC curve (AUC) was calculated to evaluate model performance.

Results

We identified 47 and 35 variables with RF and LASSO, respectively. Twenty-one overlapping variables were finally selected for model construction: age, weight, hospital stay, total red blood cell (RBC) and total fresh frozen plasma (FFP) transfusions, New York Heart Association (NYHA) class, preoperative creatinine, left ventricular ejection fraction (LVEF), RBC count, platelet (PLT) count, prothrombin time, intraoperative autologous blood, total output, total input, aortic cross-clamp (ACC) time, postoperative white blood cell (WBC) count, aspartate aminotransferase (AST), alanine aminotransferase (ALT), PLT count, hemoglobin (Hb), and LVEF. The prediction models for infection after mitral valve surgery were established based on these variables, and they all showed excellent discrimination performance in the test set (AUC > 0.79).

Conclusions

Key features selected by machine learning methods can accurately predict infection after mitral valve surgery, guiding physicians in taking appropriate preventive measures and diminishing the infection risk.
Optimal treatment for post-MI heart failure in rats: dapagliflozin first, adding sacubitril-valsartan 2 weeks later
Authors: Tao Wenqi , Yang Xiaoyu , Zhang Qing , Bi Shuli , Yao Zhuhua
Published: June 2023

Background

Based on previous research, both dapagliflozin (DAPA) and sacubitril-valsartan (S/V) improve the prognosis of patients with heart failure (HF). Our study aims to investigate whether the early initiation of DAPA or the combination of DAPA with S/V in different orders would exert a greater protective effect on heart function than that of S/V alone in post-myocardial infarction HF (post-MI HF).

Methods

Rats were randomized into six groups: (A) Sham; (B) MI; (C) MI + S/V (1st d); (D) MI + DAPA (1st d); (E) MI + S/V (1st d) + DAPA (14th d); (F) MI + DAPA (1st d) + S/V (14th d). The MI model was established in rats via surgical ligation of the left anterior descending coronary artery. Histology, Western blotting, RNA-seq, and other approaches were used to explore the optimal treatment to preserve the heart function in post-MI HF. A daily dose of 1 mg/kg DAPA and 68 mg/kg S/V was administered.

Results

The results of our study revealed that DAPA or S/V substantially improved the cardiac structure and function. DAPA and S/V monotherapy resulted in comparable reduction in infarct size, fibrosis, myocardium hypertrophy, and apoptosis. The administration of DAPA followed by S/V results in a superior improvement in heart function in rats with post-MI HF than those in other treatment groups. The administration of DAPA following S/V did not result in any additional improvement in heart function as compared to S/V monotherapy in rats with post-MI HF. Our findings further suggest that the combination of DAPA and S/V should not be administered within 3 days after acute myocardial infarction (AMI), as it resulted in a considerable increase in mortality. Our RNA-Seq data revealed that DAPA treatment after AMI altered the expression of genes related to myocardial mitochondrial biogenesis and oxidative phosphorylation.

Conclusions

Our study revealed no notable difference in the cardioprotective effects of singular DAPA or S/V in rats with post-MI HF. Based on our preclinical investigation, the most effective treatment strategy for post-MI HF is the administration of DAPA during the 2 weeks, followed by the addition of S/V to DAPA later. Conversely, adopting a therapeutic scheme whereby S/V was administered first, followed by later addition of DAPA, failed to further improve the cardiac function compared to S/V monotherapy.
The potential of an exam villa as a structural resource during prolonged exam preparation at university
Authors: Reschke Tom , Lobinger Thomas , Reschke Katharina
Published: June 2023

Introduction

Drawing on the Demand-Control Model, this study examined whether using an exam villa as a supportive learning environment provides a structural resource for law students during exam preparation. First, we hypothesized that villa students show higher decision latitude and satisfaction and less stress compared to non-villa students. Second, we expected villa use to predict stress and satisfaction over and beyond the demand-control dimensions. Third, decision latitude was tested to mediate of the relationship between villa use and both outcomes.

Methods

Our cross-sectional study included N = 205 advanced law students that gave self-reports on their stress and satisfaction, villa use, their perceived demands and decision latitude along with some control variables. All students were within a period of long-lasting exam preparation stretching over 12 to 18 months whereas n = 41 students used the villa.

Results

Using the exam villa was associated with both less subjective stress and more satisfaction. SEMs revealed villa use to predict stress but not satisfaction over and beyond the demand-control dimensions with 73% of overall explained variance in stress and 62% of variance in satisfaction. Decision latitude mediated the relationship between villa use and both outcomes.

Discussion

The findings support the potential of structural resources in reducing stress among students undergoing prolonged academic stress.
Paediatric Wolfram syndrome Type 1: should gonadal dysfunction be part of the diagnostic criteria?
Authors: Frontino Giulio , Di Tonno Raffaella , Stancampiano Marianna Rita , Arrigoni Francesca , Rigamonti Andrea , Morotti Elisa , Canarutto Daniele , Bonfanti Riccardo , Russo Gianni , Barera Graziano , Piemonti Lorenzo
Published: June 2023

Aims

Wolfram Syndrome Spectrum Disorder (WFS1-SD), in its “classic” form, is a rare autosomal recessive disease with poor prognosis and wide phenotypic spectrum. Insulin dependent diabetes mellitus (DM), optic atrophy (OA) diabetes insipidus (DI) and sensorineural deafness (D) are the main features of WFS1-SD. Gonadal dysfunction (GD) has been described mainly in adults with variable prevalence and referred to as a minor clinical feature. This is the first case series investigating gonadal function in a small cohort of paediatric patients affected by WFS1-SD.

Methods

Gonadal function was investigated in eight patients (3 male and 5 female) between 3 and 16 years of age. Seven patients have been diagnosed with classic WFS1-SD and one with non-classic WFS1-SD. Gonadotropin and sex hormone levels were monitored, as well as markers of gonadal reserve (inhibin-B and anti-Mullerian hormone). Pubertal progression was assessed according to Tanner staging.

Results

Primary hypogonadism was diagnosed in 50% of patients (n=4), more specifically 67% (n=2) of males and 40% of females (n=2). Pubertal delay was observed in one female patient. These data confirm that gonadal dysfunction may be a frequent and underdiagnosed clinical feature in WFS1-SD.

Conclusions

GD may represent a frequent and earlier than previously described feature in WFS1-SD with repercussions on morbidity and quality of life. Consequently, we suggest that GD should be included amongst clinical diagnostic criteria for WFS1-SD, as has already been proposed for urinary dysfunction. Considering the heterogeneous and elusive presentation of WFS1-SD, this clinical feature may assist in an earlier diagnosis and timely follow-up and care of treatable associated diseases (i.e. insulin and sex hormone replacement) in these young patients.
Voltage and frequency instability in large PV systems connected to weak power grid
Authors: Zhang Qianjin , Hu Qi , Sun Siwei , Mei Dikui , Liu Sucheng , Liu Xiaodong
Published: June 2023

The voltage and frequency control of photovoltaic (PV) systems are influenced by coupled nonlinear factors. It has been discovered that frequency control stability is threatened by voltage regulation methods in PV systems. However, the frequency instability caused by voltage regulation methods has not been fully investigated. This paper investigates the voltage and frequency stability problems in PV systems connected with weak power grids. The voltage problems caused by grid impedance, comprising inverter AC voltage and DC voltage, are first analyzed. Then, methods for improving voltage stability, such as reactive power compensation, and the benefits and drawbacks of various compensation methods are investigated. Finally, the effect of reactive power compensation on frequency control stability is investigated and resolved. Simulations and experiments are used to validate the theory’s correctness.
A novel ACE2 decoy for both neutralization of SARS-CoV-2 variants and killing of infected cells
Authors: Kegler Alexandra , Drewitz Laura , Arndt Claudia , Daglar Cansu , Rodrigues Loureiro Liliana , Mitwasi Nicola , Neuber Christin , González Soto Karla Elizabeth , Bartsch Tabea , Baraban Larysa , Ziehr Holger , Heine Markus , Nieter Annabel , Moreira-Soto Andres , Kühne Arne , Drexler Jan Felix , Seliger Barbara , Laube Markus , Máthé Domokos , Pályi Bernadett , Hajdrik Polett , Forgách László , Kis Zoltán , Szigeti Krisztián , Bergmann Ralf , Feldmann Anja , Bachmann Michael
Published: June 2023

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) led to millions of infections and deaths worldwide. As this virus evolves rapidly, there is a high need for treatment options that can win the race against new emerging variants of concern. Here, we describe a novel immunotherapeutic drug based on the SARS-CoV-2 entry receptor ACE2 and provide experimental evidence that it cannot only be used for (i) neutralization of SARS-CoV-2 in vitro and in SARS-CoV-2-infected animal models but also for (ii) clearance of virus-infected cells. For the latter purpose, we equipped the ACE2 decoy with an epitope tag. Thereby, we converted it to an adapter molecule, which we successfully applied in the modular platforms UniMAB and UniCAR for retargeting of either unmodified or universal chimeric antigen receptor-modified immune effector cells. Our results pave the way for a clinical application of this novel ACE2 decoy, which will clearly improve COVID-19 treatment.
Early on-treatment plasma interleukin-18 as a promising indicator for long-term virological response in patients with HIV-1 infection
Authors: Lin Weiyin , Li Liya , Guo Pengle , He Yaozu , He Haolan , Li Hong , Zhong Huolin , Liu Cong , Du Peishan , Cai Weiping , Tang Xiaoping , Li Linghua
Published: June 2023

Background and aims

It is necessary to identify simple biomarkers that can efficiently predict the efficacy of long-term antiretroviral therapy (ART) against human immunodeficiency virus (HIV), especially in underdeveloped countries. We characterized the dynamic changes in plasma interleukin-18 (IL-18) and assessed its performance as a predictor of long-term virological response.

Methods

This was a retrospective cohort study of HIV-1-infected patients enrolled in a randomized controlled trial with a follow-up of 144  weeks of ART. Enzyme-linked immunosorbent assay was performed to evaluate plasma IL-18. Long-term virological response was defined as HIV-1 RNA <20 copies/mL at week 144.

Results

Among the 173 enrolled patients, the long-term virological response rate was 93.1%. Patients with a long-term virological response had significantly lower levels of week 24 IL-18 than non-responders. We defined 64  pg./mL, with a maximum sum of sensitivity and specificity, as the optimal cutoff value of week 24 IL-18 level to predict long-term virological response. After adjusting for age, gender, baseline CD4+ T-cell count, baseline CD4/CD8 ratio, baseline HIV-1 RNA level, HIV-1 genotype and treatment strategy, we found that lower week 24 IL-18 level (≤64 vs. >64 pg./mL, a OR 19.10, 95% CI: 2.36–154.80) was the only independent predictor of long-term virological response.

Conclusion

Early on-treatment plasma IL-18 could act as a promising indicator for long-term virological response in patients with HIV-1 infection. Chronic immune activation and inflammation may represent a potential mechanism; further validation is necessary.
Endophytic Bacillus atrophaeus CHGP13 and salicylic acid inhibit blue mold of lemon by regulating defense enzymes
Authors: Maalik Saba , Moosa Anam , Zulfiqar Faisal , Aslam Muhammad Naveed , Mahmood Tahir , Siddique Kadambot H. M.
Published: June 2023

Lemons (Citrus limon L.) are one of the most economically important and consumed fruit worldwide. The species is vulnerable to several postharvest decay pathogens, of which Penicillium italicum associated with blue mold disease is the most damaging. This study investigates the use of integrated management for blue mold of lemon using lipopeptides (LPs) extracted from endophytic Bacillus strains and resistance inducers. Two resistance inducers; salicylic acid (SA) and benzoic acid (BA) were tested at 2, 3, 4, and 5 mM concentrations against the development of blue mold on lemon fruit. The 5 mM SA treatment produced the lowest disease incidence (60%) and lesion diameter (1.4 cm) of blue mold on lemon fruit relative to the control. In an in vitro antagonism assay eighteen Bacillus strains were evaluated for their direct antifungal effect against P. italicum; CHGP13 and CHGP17 had the greatest inhibition zones of 2.30 and 2.14 cm. Lipopeptides (LPs) extracted from CHGP13 and CHGP17 also inhibited the colony growth of P. italicum. LPs extracted from CHGP13 and 5 mM SA were tested as single and combined treatments against disease incidence and lesion diameter of blue mold on lemon fruit. SA + CHGP13 + PI had the lowest disease incidence (30%) and lesion diameter (0.4 cm) of P. italicum on lemon fruit relative to the other treatments. Furthermore, the lemon fruit treated with SA + CHGP13 + PI had the highest PPO, POD, and PAL activities. The postharvest quality analysis of the lemon fruit including fruit firmness, total soluble solids, weight loss, titratable acidity, and ascorbic acid content revealed that the treatment SA + CHGP13 + PI had little effect on fruit quality compared to the healthy control. These findings indicate that Bacillus strains and resistance inducers can be used as components of integrated disease management for the blue mold of lemon.
Robust and language-independent acoustic features in Parkinson's disease
Authors: Scimeca Sabrina , Amato Federica , Olmo Gabriella , Asci Francesco , Suppa Antonio , Costantini Giovanni , Saggio Giovanni
Published: June 2023

Introduction

The analysis of vocal samples from patients with Parkinson's disease (PDP) can be relevant in supporting early diagnosis and disease monitoring. Intriguingly, speech analysis embeds several complexities influenced by speaker characteristics (e.g., gender and language) and recording conditions (e.g., professional microphones or smartphones, supervised, or non-supervised data collection). Moreover, the set of vocal tasks performed, such as sustained phonation, reading text, or monologue, strongly affects the speech dimension investigated, the feature extracted, and, as a consequence, the performance of the overall algorithm.

Methods

We employed six datasets, including a cohort of 176 Healthy Control (HC) participants and 178 PDP from different nationalities (i.e., Italian, Spanish, Czech), recorded in variable scenarios through various devices (i.e., professional microphones and smartphones), and performing several speech exercises (i.e., vowel phonation, sentence repetition). Aiming to identify the effectiveness of different vocal tasks and the trustworthiness of features independent of external co-factors such as language, gender, and data collection modality, we performed several intra- and inter-corpora statistical analyses. In addition, we compared the performance of different feature selection and classification models to evaluate the most robust and performing pipeline.

Results

According to our results, the combined use of sustained phonation and sentence repetition should be preferred over a single exercise. As for the set of features, the Mel Frequency Cepstral Coefficients demonstrated to be among the most effective parameters in discriminating between HC and PDP, also in the presence of heterogeneous languages and acquisition techniques.

Conclusion

Even though preliminary, the results of this work can be exploited to define a speech protocol that can effectively capture vocal alterations while minimizing the effort required to the patient. Moreover, the statistical analysis identified a set of features minimally dependent on gender, language, and recording modalities. This discloses the feasibility of extensive cross-corpora tests to develop robust and reliable tools for disease monitoring and staging and PDP follow-up.
Prenatal alcohol and tetrahydrocannabinol exposure: Effects on spatial and working memory
Authors: Lei Annie , Breit Kristen R. , Thomas Jennifer D.
Published: June 2023

Introduction

Alcohol and cannabis are widely used recreational drugs that can negatively impact fetal development, leading to cognitive impairments. However, these drugs may be used simultaneously and the effects of combined exposure during the prenatal period are not well understood. Thus, this study used an animal model to investigate the effects of prenatal exposure to ethanol (EtOH), Δ-9-tetrahydrocannabinol (THC), or the combination on spatial and working memory.

Methods

Pregnant Sprague–Dawley rats were exposed to vaporized ethanol (EtOH; 68 ml/h), THC (100 mg/ml), the combination, or vehicle control during gestational days 5–20. Adolescent male and female offspring were evaluated using the Morris water maze task to assess spatial and working memory.

Results

Prenatal THC exposure impaired spatial learning and memory in female offspring, whereas prenatal EtOH exposure impaired working memory. The combination of THC and EtOH did not exacerbate the effects of either EtOH or THC, although subjects exposed to the combination were less thigmotaxic, which might represent an increase in risk-taking behavior.

Discussion

Our results highlight the differential effects of prenatal exposure to THC and EtOH on cognitive and emotional development, with substance- and sex-specific patterns. These findings highlight the potential harm of THC and EtOH on fetal development and support public health policies aimed at reducing cannabis and alcohol use during pregnancy.
Generative AI for brain image computing and brain network computing: a review
Authors: Gong Changwei , Jing Changhong , Chen Xuhang , Pun Chi Man , Huang Guoli , Saha Ashirbani , Nieuwoudt Martin , Li Han-Xiong , Hu Yong , Wang Shuqiang
Published: June 2023

Recent years have witnessed a significant advancement in brain imaging techniques that offer a non-invasive approach to mapping the structure and function of the brain. Concurrently, generative artificial intelligence (AI) has experienced substantial growth, involving using existing data to create new content with a similar underlying pattern to real-world data. The integration of these two domains, generative AI in neuroimaging, presents a promising avenue for exploring various fields of brain imaging and brain network computing, particularly in the areas of extracting spatiotemporal brain features and reconstructing the topological connectivity of brain networks. Therefore, this study reviewed the advanced models, tasks, challenges, and prospects of brain imaging and brain network computing techniques and intends to provide a comprehensive picture of current generative AI techniques in brain imaging. This review is focused on novel methodological approaches and applications of related new methods. It discussed fundamental theories and algorithms of four classic generative models and provided a systematic survey and categorization of tasks, including co-registration, super-resolution, enhancement, classification, segmentation, cross-modality, brain network analysis, and brain decoding. This paper also highlighted the challenges and future directions of the latest work with the expectation that future research can be beneficial.
Homocysteine—a retrospective and prospective appraisal
Authors: McCaddon Andrew , Miller Joshua W.
Published: June 2023

The biologically important amino acid homocysteine links sulfur, methionine, and one-carbon metabolism. This review describes its initial discovery, the identification of the clinical condition of “homocystinuria” and the recognition of its close relationship to folate and vitamin B12 metabolism. It discusses the history behind its current association with diverse diseases including neural tube defects, cardio- and cerebrovascular disease and, more recently, dementia and Alzheimer’s Disease. It also explores current controversies and considers potential future research directions. It is intended to give a general overview of homocysteine in relation to health and disease.
Effect of iron-fortified jamun leather on the Asunra-induced anemia in Sprague Dawley rats
Authors: Naz Nosheen , Khan Moazzam Rafiq , Shabbir Muhammad Asim , Faisal Muhammad Naeem
Published: June 2023

Introduction

Micronutrients such as minerals and vitamins are required in a minute quantity but play a pivotal role in the functioning of the body. Therefore, deficiency in one of them can lead to lethal health conditions. Iron deficiency anaemia is one of the most common micronutrient deficiencies across the world and is affecting women and children.

Methods

The present study aimed to investigate the anti-anaemic effect of fortified jamun leather on anaemia biomarkers and haematology in anaemic female Sprague Dawley rats. A total of 40 Sprague Dawley rats were used in 4 groups. Iron deficiency anaemia was induced by oral administration of the Asunra drug. The treatments were fed at two dosage levels i.e., 40 and 60% iron-fortified leather. All animals were treated for 60 days and the parameters including biochemical, and histopathology of the kidney and liver were examined.

Results

The experiment's findings showed that the group fed with iron-fortified leather (G3) succeeded significantly (P < 0.05) in restoring the serum iron (98.68 ± 2.88 μg/dL), haemoglobin (12.41 ± 0.32 g/dL), ferritin (24.54 ± 1.98 ng/mL) and haematocrit levels (39.30 ± 1.66%) at the end of the 60 days period. Additionally, the treated group's mean values for transferrin and total iron binding capacity were lower than those of the anaemic rats, indicating an improvement in iron levels. The microscopic analysis revealed that treatments had no toxic effects on the kidney and liver tissues, except in the diseased group, which had necrosis and irregular cell structure.

Conclusion

Conclusively, iron-fortified jamun leather helped improve iron deficiency biomarkers and imparted a non-toxic effect on tissues in rats.
Identification of cardiotoxicity related to non-small cell lung cancer (NSCLC) treatments: A systematic review
Authors: Chan Stefanie Ho Yi , Khatib Yasmin , Webley Sherael , Layton Deborah , Salek Sam
Published: June 2023

Introduction: In the last few decades, there has been a rapid development in cancer therapies and improved detection strategies, hence the death rates caused by cancer have decreased. However, it has been reported that cardiovascular disease has become the second leading cause of long-term morbidity and fatality among cancer survivors. Cardiotoxicity from anticancer drugs affects the heart’s function and structure and can occur during any stage of the cancer treatments, which leads to the development of cardiovascular disease.

Objectives: To investigate the association between anticancer drugs for non-small cell lung cancer (NSCLC) and cardiotoxicity as to whether: different classes of anticancer drugs demonstrate different cardiotoxicity potentials; different dosages of the same drug in initial treatment affect the degree of cardiotoxicity; and accumulated dosage and/or duration of treatments affect the degree of cardiotoxicity.

Methods: This systematic review included studies involving patients over 18 years old with NSCLC and excluded studies in which patients’ treatments involve radiotherapy only. Electronic databases and registers including Cochrane Library, National Cancer Institute (NCI) Database, PubMed, Scopus, Web of Science, ClinicalTrials.gov and the European Union Clinical Trials Register were systematically searched from the earliest available date up until November 2020. A full version protocol of this systematic review (CRD42020191760) had been published on PROSPERO.

Results: A total of 1785 records were identified using specific search terms through the databases and registers; 74 eligible studies were included for data extraction. Based on data extracted from the included studies, anticancer drugs for NSCLC that are associated with cardiovascular events include bevacizumab, carboplatin, cisplatin, crizotinib, docetaxel, erlotinib, gemcitabine and paclitaxel. Hypertension was the most reported cardiotoxicity as 30 studies documented this cardiovascular adverse event. Other reported treatment-related cardiotoxicities include arrhythmias, atrial fibrillation, bradycardia, cardiac arrest, cardiac failure, coronary artery disease, heart failure, ischemia, left ventricular dysfunction, myocardial infarction, palpitations, and tachycardia.

Conclusion: The findings of this systematic review have provided a better understanding of the possible association between cardiotoxicities and anticancer drugs for NSCLC. Whilst variation is observed across different drug classes, the lack of information available on cardiac monitoring can result in underestimation of this association.

Systematic Review Registration:https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42020191760, identifier PROSPERO CRD42020191760.
The simulation of terahertz waves transmission in the arc plasma
Authors: Wang Hailu , Yao Jingfeng , Fang Chuan , Yuan Chengxun , Li He-Ping
Published: June 2023

The diagnostic of high-density hot plasma is a challenging task due to its high temperature and electron density. Arc plasma is one of the typical hot density plasmas, and its diagnosis is the key to develop its new applications. In this paper, the temperature and density distributions of welding plasmas with different discharge currents are numerically simulated based on a Tungsten Inert Gas Arc Welding model, and the electron density distributions are calculated. Then propagation properties of broadband terahertz (THz) waves in the modeling arc jets are calculated by the finite difference time domain method. These results not only provide a preliminary theoretical guidance for in-depth understanding the problems of blackout in re-entry communication, but also develop a new idea for the terahertz diagnostic of plasma with high density.
Aquaporins and CO2 diffusion across biological membrane
Authors: Chen Junyu , Yue Ke , Shen Lulu , Zheng Chuncui , Zhu Yiyong , Han Kun , Kai Lei
Published: June 2023

Despite the physiological significance of effective CO2 diffusion across biological membranes, the underlying mechanism behind this process is not yet resolved. Particularly debatable is the existence of CO2-permeable aquaporins. The lipophilic characteristic of CO2 should, according to Overton’s rule, result in a rapid flux across lipid bilayers. However, experimental evidence of limited membrane permeability poses a challenge to this idea of free diffusion. In this review, we summarized recent progress with regard to CO2 diffusion, and discussed the physiological effects of altered aquaporin expression, the molecular mechanisms of CO2 transport via aquaporins, and the function of sterols and other membrane proteins in CO2 permeability. In addition, we highlight the existing limits in measuring CO2 permeability and end up with perspectives on resolving such argument either by determining the atomic resolution structure of CO2 permeable aquaporins or by developing new methods for measuring permeability.
Proteins journey—from marine to freshwater ecosystem: blood plasma proteomic profiles of pink salmon Oncorhynchus gorbuscha Walbaum, 1792 during spawning migration
Authors: Kochneva Albina , Efremov Denis , Murzina Svetlana A.
Published: June 2023

The pink salmon (Oncorhynchus gorbuscha) is a commercial anadromous fish species of the family Salmonidae. This species has a 2-year life cycle that distinguishes it from other salmonids. It includes the spawning migration from marine to freshwater environments, accompanied by significant physiological and biochemical adaptive changes in the body. This study reveals and describes variability in the blood plasma proteomes of female and male pink salmon collected from three biotopes—marine, estuarine and riverine—that the fish pass through in spawning migration. Identification and comparative analysis of blood plasma protein profiles were performed using proteomics and bioinformatic approaches. The blood proteomes of female and male spawners collected from different biotopes were qualitatively and quantitatively distinguished. Females differed primarily in proteins associated with reproductive system development (certain vitellogenin and choriogenin), lipid transport (fatty acid binding protein) and energy production (fructose 1,6-bisphosphatase), and males in proteins involved in blood coagulation (fibrinogen), immune response (lectins) and reproductive processes (vitellogenin). Differentially expressed sex-specific proteins were implicated in proteolysis (aminopeptidases), platelet activation (β- and γ-chain fibrinogen), cell growth and differentiation (a protein containing the TGF_BETA_2 domain) and lipid transport processes (vitellogenin and apolipoprotein). The results are of both fundamental and practical importance, adding to existing knowledge of the biochemical adaptations to spawning of pink salmon, a representative of economically important migratory fish species.
Validation of the Malay-Version of Recovery Knowledge Inventory among mental health providers in Malaysia
Authors: Lakshman Stella Jane , Mohd Daud Tuti Iryani , Midin Marhani , Ahmad Farah , Tommy Kezia
Published: June 2023

Background

The Recovery Knowledge Inventory (RKI) is a widely used self-report instrument that assesses recovery-oriented knowledge among mental health professionals. The purpose of this study is to translate the RKI into the Malay language (RKI-M) and to examine its psychometric properties among Malaysian health care workers.

Methods

A cross-sectional study involving 143 participants was conducted at an urban teaching hospital, an urban government hospital, and a rural government hospital. Following the translation of the RKI, its internal reliability was determined using Cronbach’s alpha. Construct validity was also determined using confirmatory factor analysis.

Results

The Malay-Version RKI (RKI-M) has good internal reliability with a Cronbach’s alpha of 0.83. However, the Malay-version RKI failed to replicate the original four-factor structure. The final model only achieved the best model fit after the removal of 9 items with two-factor loadings: (GFI = 0.92; AGFI = 0 0.87; CFI = 0.91; RMSEA = 0.074).

Conclusion

The 20-item RKI-M is reliable but has poor construct validity. However, the modified 11-item Malay-version RKI is a more reliable measure as it has good construct validity, with room for future studies to examine the psychometric properties of the modified 11-item RKI among mental health care workers. More training on recovery knowledge should be done, and a simple worded questionnaire should be developed in keeping with local practitioners.
The COVID-19 pandemic impact on the Polish medical personnel work: a survey and in-depth interviews study
Authors: Przyłęcki Paweł , Wieczorkowska Magdalena , Pawlak-Kałuzińska Agnieszka , Cedrowska-Adamus Wioletta , Gulczyńska Ewa
Published: June 2023

Objective

The objective of the study was to examine the impact of the COVID-19 pandemic on the work of medical personnel in terms of: task scope, preparation to perform medical tasks related to the pandemic, team collaboration, involvement in tasks performed, concerns about performing tasks related to the pandemic, stress levels.

Methods

The mixed-method approach was applied to this cross-sectional study. The online questionnaire which included 40 questions was completed via Google among medical personnel in Poland. Eight semi-structured, in-depth interviews were conducted to deepen the data obtained with the questionnaires.

Participants

The questionnaire was completed by 215 healthcare professionals, with the largest group being nurses (56.3%) followed by physicians (22.3%), midwives (11.6%) and other healthcare professionals (e.g., physiotherapists, paramedics, nutritionists – 9.8%). Among the respondents were people who worked in the hospital in the so-called “covid wards” (31.2%) and other hospital wards (60%) as well as people who were employed outside the hospital (8.8%).

Results

The pandemic affected the nature and range of tasks performed by health professionals. Initially, respondents felt unprepared to work under pandemic conditions, but over time their ratings increased in all areas studied. More than half of respondents reported no change in interpersonal relationship within the team, but nearly 35% noted a worsening and only one in 10 claimed improvement. Study participants rated their own commitment to tasks slightly higher than that of their colleagues (mean 4.9 and 4.4 respectively) but the overall rating was high. The mean self-rating of work stress increased from 3.7 before the pandemic to 5.1 during the pandemic. Most of the respondents were afraid of transmission of the infection to their relatives. Other fears included the possibility of making a medical error, not being able to help the patient, not having enough personal protective equipment (PPE) and contracting SARS-CoV-2.

Conclusion

The conducted study revealed that the organization of medical care in the initial period of the pandemic, especially the hospital care of patients infected with SARS-CoV-2, was quite chaotic. The most affected were the people who were transferred to work in the covid wards. Not all medical professionals were prepared to work with the COVID-19 patients, as they lacked experience working in such facilities, especially in intensive care units (ICU). Working under time pressure and under new conditions led mainly to an increase in perceived stress and conflicts between staff.
Central and peripheral changes in the retina and choroid in patients with diabetes mellitus without clinical diabetic retinopathy assessed by ultra-wide-field optical coherence tomography angiography
Authors: Zhao Qing , Wang Chuting , Meng Lihui , Cheng Shiyu , Gu Xingwang , Chen Youxin , Zhao Xinyu
Published: June 2023

Background

To explore the central and peripheral retinal and choroidal changes in diabetic patients without clinical diabetic retinopathy (DM-NoDR) using ultra-wide-field swept-source optical coherence tomography angiography (UWF-SS-OCTA).

Methods

67 DM-NoDR eyes and 32 age-matched healthy eyes were recruited. Retinal and choroidal parameters, including qualitative retinal microangiopathy, vessel flow (VFD) and linear density (VLD), thickness, and volume, were measured in the central and peripheral areas of the 24 × 20 mm2 UWF-SS-OCTA images.

Results

DM-NoDR eyes had significantly more nonperfusion area and capillary tortuosity than controls in the central and peripheral areas (p < 0.05). The presence of central capillary tortuosity was associated with higher levels of serum creatinine (OR 1.049, 95%CI 1.001–1.098; p = 0.044) and blood urea nitrogen (OR 1.775, 95%CI 1.051–2.998; p = 0.032) in DM-NoDR eyes. For DM-NoDR eyes versus controls, VFD in the 300-μm annulus around the foveal avascular zone, superficial capillary plexus (SCP), and full retina, and SCP-VLD significantly decreased, while VFD in the deep capillary plexus (DCP), retinal thickness, and retinal volume increased (p < 0.05). Analysis in the central and peripheral areas recapitulated all these findings, except for decreased peripheral thickness and volume and no difference in peripheral DCP-VFD. In DM-NoDR eyes, choriocapillaris-VFD, choroidal thickness, and choroidal volume increased in the central area, while VFD in the large and medium choroidal vessel layer decreased in the whole image (p < 0.05).

Conclusion

Retinal and choroidal changes already existed in the central and/or peripheral areas of DM-NoDR eyes. UWF-SS-OCTA, enabling the visualization of the peripheral fundus area, is a promising image technique for the early detection of fundus changes in DM-NoDR patients.
Adherence to the physical activity guideline beyond the recommended minimum weekly amount: impacts on indicators of physical function in older adults
Authors: Veen Jort , Edholm Peter , Rodriguez-Zamora Lara , Folkesson Mattias , Kadi Fawzi , Nilsson Andreas
Published: June 2023

Introduction

The extent to which additional health benefits of accumulating twice the minimum amount of time in moderate-to-vigorous physical activity (MVPA) affects indicators of physical function in older adults is unclear. Therefore, the aim of the present study was to assess indicators of physical function in older adults who accumulate at least 150 but less than 300 min/week of MVPA compared to those accumulating at least 300 min/week.

Methods

Indicators of physical function, including handgrip strength, 5 times sit-to-stand test (5-STS), squat jump and 6-min walk test (6MWT) were assessed in a sample of 193 older men (n = 71, 67 ± 2 years), and women (n = 122, 67 ± 2 years), who all accumulated at least 150 weekly minutes of MVPA. Time in MVPA was assessed by accelerometry during 1 week and engagement in muscle strengthening activities (MSA) was assessed by self-report. Protein intake was assessed by a food-frequency-questionnaire. Participants were classified as physically active (≥150 but <300 min of MVPA per week) or as highly physically active (≥300 min of MVPA per week).

Results

Factorial analysis of variance revealed that older adults accumulating at least 300 min of MVPA per week had a significantly (p < 0.05) better 6MWT performance and overall physical function compared to the less active group. These findings remained significant after further adjustment for MSA, sex, waist circumference and protein intake. In contrast, no significant differences in indicators of muscle strength were observed between the two groups.

Discussion

Adherence to twice the recommended minimum amount of weekly MVPA time is related to a better physical function, evidenced by a better walking performance compared to adherence to the minimum weekly amount of MVPA. This finding emphasizes the benefits of accumulating daily MVPA beyond the minimum recommended amount to optimize the ability to perform activities of daily living, thus reducing the burden of physical disability and related health-care costs.