bluuebunny/crossref_metadata_2025 huggingface dataset:
Vancomycin-Resistant Enterococci and Extended-Spectrum β-Lactamase-Producing Bacterial Colonization of the Cervix after Emergency Cerclage: Is It Safe?
Authors: Jang Won-Kyu , Bae Jin-Gon
Published: August 2021

Antimicrobial resistance is currently becoming a global threat to human health. We performed a retrospective study on patients who underwent emergency cerclage between January 2016 and December 2018 at the Dongsan Medical Center. Cervical culture was first performed before surgery to confirm that there was no infection and was repeated on days 1, 4, and 7 after surgery. A total of 85 pregnant women underwent emergency cerclage. Among them, six patients had vancomycin-resistant enterococci (VRE) colonization in the cervix after cerclage, and 23 patients developed extended-spectrum β-lactamase (ESBL)-producing bacterial colonization in the cervix. The average gestational age at delivery was lower in the VRE group. Neonatal death was also significantly higher in the VRE group. The rate of occurrence of early-onset sepsis was also higher in the VRE group, and both VRE and ESBL-producing bacterial colonization cases in which early-onset sepsis occurred resulted in neonatal death. The prognosis of cervical VRE colonization after cervical surgery was poor, whereas the prognosis of ESBL-producing bacterial colonization in the cervix did not differ significantly from that of the control group. However, careful neonatal treatment is required considering that early-onset sepsis is fatal to the newborn.
Non-β-Lactam Allosteric Inhibitors Target Methicillin-Resistant Staphylococcus aureus: An In Silico Drug Discovery Study
Authors: Ibrahim Mahmoud A. A. , Abdeljawaad Khlood A. A. , Abdelrahman Alaa H. M. , Alzahrani Othman R. , Alshabrmi Fahad M. , Khalaf Esraa , Moustafa Mahmoud F. , Alrumaihi Faris , Allemailem Khaled S. , Soliman Mahmoud E. S. , Paré Paul W. , Hegazy Mohamed-Elamir F. , Atia Mohamed A. M.
Published: August 2021

Penicillin-binding proteins (PBPs) catalyze the final stages for peptidoglycan cell-wall bio-synthesis. Mutations in the PBP2a subunit can attenuate β-lactam antibiotic activity, resulting in unimpeded cell-wall formation and methicillin-resistant Staphylococcus aureus (MRSA). A double mutation in PBP2a (i.e., N146K and E150K) is resistant to β-lactam inhibitors; however, (E)-3-(2-(4-cyanostyryl)-4-oxoquinazolin-3(4H)-yl) benzoic acid (QNZ), a heterocyclic antibiotic devoid of a β-lactam ring, interacts non-covalently with PBP2a allosteric site and inhibits PBP enzymatic activity. In the search for novel inhibitors that target this PBP2a allosteric site in acidic medium, an in silico screening was performed. Chemical databases including eMolecules, ChEMBL, and ChEBI were virtually screened for candidate inhibitors with a physicochemical similarity to QNZ. PBP2a binding affinities from the screening were calculated based on molecular docking with co-crystallized ligand QNZ serving as a reference. Molecular minimization calculations were performed for inhibitors with docking scores lower than QNZ (calc. −8.3 kcal/mol) followed by combined MD simulations and MM-GBSA binding energy calculations. Compounds eMol26313223 and eMol26314565 exhibited promising inhibitor activities based on binding affinities (ΔGbinding) that were twice that of QNZ (−38.5, −34.5, and −15.4 kcal/mol, respectively). Structural and energetic analyses over a 50 ns MD simulation revealed high stability for the inhibitors when complexed with the double mutated PBP2a. The pharmacokinetic properties of the two inhibitors were predicted using an in silico ADMET analysis. Calculated binding affinities hold promise for eMol26313223 and eMol26314565 as allosteric inhibitors of PBP2a in acidic medium and establish that further in vitro and in vivo inhibition experimentation is warranted.
ESBL-Producing, Carbapenem- and Ciprofloxacin-Resistant Escherichia coli in Belgian and Dutch Broiler and Pig Farms: A Cross-Sectional and Cross-Border Study
Authors: De Koster Sien , Ringenier Moniek , Lammens Christine , Stegeman Arjan , Tobias Tijs , Velkers Francisca , Vernooij Hans , Kluytmans-van den Bergh Marjolein , Kluytmans Jan , Dewulf Jeroen , Goossens Herman ,   on behalf of the i-4-1-Health Study Group
Published: August 2021

Background. The use of antibiotics in food production selects for resistant bacteria and may cause a threat to human and animal health. Belgium and the Netherlands have one of the highest densities of broilers and pigs in Europe, making active monitoring of antibiotic use and resistance in this region vital. Objectives. This study aimed to quantify ESBL-producing (ESBL-E. coli), carbapenem- and ciprofloxacin-resistant (CiproR) Escherichia coli in animal feces on broiler and pig farms with a history of high antibiotic use in Belgium and the Netherlands. Methods. A total of 779 broiler and 817 pig fecal samples, collected from 29 conventional broiler and 31 multiplier pig farms in the cross-border region of Belgium and the Netherlands, were screened for the presence of antibiotic-resistant E. coli using selective culturing. Results. Carbapenem-resistant E. coli were not detected. ESBL-E. coli were remarkably more prevalent in samples from Belgian than Dutch farms. However, CiproR-E. coli were highly prevalent in broilers of both countries. The percentage of samples with ESBL- and CiproR-E. coli was lower in pig compared to poultry farms and varied between farms. No clear association with the on-farm antibiotic use in the year preceding sampling was observed. Multidrug resistance was frequently observed in samples from both countries, but ESBL-production in combination with ciprofloxacin resistance was higher in samples from Belgium. Conclusions. This study demonstrated marked differences in antibiotic resistance between countries, farms and within farms. The observed variation cannot be explained straightforward by prior quantity of antibiotic use suggesting that it results from more complex interactions that warrant further investigation.
Opportunities for Nanomedicine in Clostridioides difficile Infection
Authors: Wang Pei-Wen , Lee Wei-Ting , Wu Ya-Na , Shieh Dar-Bin
Published: August 2021

Clostridioides difficile, a spore-forming bacterium, is a nosocomial infectious pathogen which can be found in animals as well. Although various antibiotics and disinfectants were developed, C. difficile infection (CDI) remains a serious health problem. C. difficile spores have complex structures and dormant characteristics that contribute to their resistance to harsh environments, successful transmission and recurrence. C. difficile spores can germinate quickly after being exposed to bile acid and co-germinant in a suitable environment. The vegetative cells produce endospores, and the mature spores are released from the hosts for dissemination of the pathogen. Therefore, concurrent elimination of C. difficile vegetative cells and inhibition of spore germination is essential for effective control of CDI. This review focused on the molecular pathogenesis of CDI and new trends in targeting both spores and vegetative cells of this pathogen, as well as the potential contribution of nanotechnologies for the effective management of CDI.
Oxidative Stress Does Not Influence Subjective Pain Sensation in Inflammatory Bowel Disease Patients
Authors: Zielińska Anna Krystyna , Sałaga Maciej , Siwiński Paweł , Włodarczyk Marcin , Dziki Adam , Fichna Jakub
Published: August 2021

Oxidative stress (OS) has been proposed as a significant causative and propagating factor in inflammatory bowel diseases (IBDs). Modulation of OS is possible through antioxidants and inhibition of oxidizing enzymes. Thirty-one IBD patients and thirty-two controls were included in the study. The aim was to examine the levels of OS in colonic tissue of IBD requiring surgical intervention and control group, and their association with pain intensity. Total antioxidant capacity (TAC), superoxide dismutase (SOD) and catalase (CAT) activity, glutathione (GSH) and oxidized glutathione (GSSG) levels, and glutathione peroxidase (GPX) activity as markers of antioxidant defense were determined. Cyclooxygenases activities (Total COX, COX-1 and COX-2) were measured as prooxidant enzymes. Thiobarbituric acid reactive substances (TBARS) concentrations were measured to evaluate lipid peroxidation. Disease activity was assessed, and each subject filled out VAS and Laitinen’s pain assessment scales. Correlation between the OS, pain intensity, disease activity parameters, C-reactive protein (CRP), number of stools passed daily, disease duration, and dietary habits was investigated. No TAC differences were found between the groups. A significant decrease of SOD activity and GSH and GSSG levels was seen in IBD patients vs. controls, while GPX activity was diminished significantly only in CD patients. CAT and COX-1 activity was increased, and COX-2 significantly decreased in IBD. TBARS were significantly higher in CD patients compared to control group. No correlation was found between pain scores, inflammatory status, disease activity, disease duration, or dietary habits and OS markers. In our study, OS did not influence pain sensation reported by IBD patients.
Polyphenol Intake and Epithelial Ovarian Cancer Risk in the European Prospective Investigation into Cancer and Nutrition (EPIC) Study
Authors: Londoño Catalina , Cayssials Valerie , de Villasante Izar , Crous-Bou Marta , Scalbert Augustin , Weiderpass Elisabete , Agudo Antonio , Tjønneland Anne , Olsen Anja , Overvad Kim , Katzke Verena , Schulze Matthias , Palli Domenico , Krogh Vittorio , Santucci de Magistris Maria , Tumino Rosario , Ricceri Fulvio , Gram Inger T. , Rylander Charlotta , Skeie Guri , Sánchez Maria-Jose , Amiano Pilar , Huerta José María , Barricarte Aurelio , Sartor Hanna , Sonestedt Emily , Esberg Anders , Idahl Annika , Mahamat-Saleh Yahya , Laouali Nasser , Kvaskoff Marina , Turzanski-Fortner Renée , Zamora-Ros Raul
Published: August 2021

Despite some epidemiological evidence on the protective effects of polyphenol intake on epithelial ovarian cancer (EOC) risk from case-control studies, the evidence is scarce from prospective studies and non-existent for several polyphenol classes. Therefore, we aimed to investigate the associations between the intake of total, classes and subclasses of polyphenols and EOC risk in a large prospective study. The study was conducted in the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort, which included 309,129 adult women recruited mostly from the general population. Polyphenol intake was assessed through validated country-specific dietary questionnaires and the Phenol-Explorer database. During a mean follow-up of 14 years, 1469 first incident EOC cases (including 806 serous, 129 endometrioid, 102 mucinous, and 67 clear cell tumours) were identified. In multivariable-adjusted Cox regression models, the hazard ratio in the highest quartile of total polyphenol intake compared with the lowest quartile (HRQ4vsQ1) was 1.14 (95% CI 0.94–1.39; p-trend = 0.11). Similarly, the intake of most classes and subclasses of polyphenols were not related to either overall EOC risk or any EOC subtype. A borderline statistically significant positive association was observed between phenolic acid intake (HRQ4vsQ1 = 1.20, 95% CI 1.01–1.43; p-trend = 0.02) and EOC risk, especially for the serous subtype and in women with obesity, although these associations did not exceed the Bonferroni correction threshold. The current results do not support any association between polyphenol intake and EOC in our large European prospective study. Results regarding phenolic acid intake need further investigation
Antioxidant Status of Broiler Chickens Fed Diets Supplemented with Vinification By-Products: A Valorization Approach
Authors: Mavrommatis Alexandros , Giamouri Elisavet , Myrtsi Eleni D. , Evergetis Epameinondas , Filippi Katiana , Papapostolou Harris , Koulocheri Sofia D. , Zoidis Evangelos , Pappas Athanasios C. , Koutinas Apostolis , Haroutounian Serkos A. , Tsiplakou Eleni
Published: August 2021

Vinification by-products display great potential for utilization as feed additives rich in antioxidant compounds. Thus, the effect of dietary ground grape pomace (GGP), wine lees extract rich in yeast cell walls (WYC), and grape stem extracts (PE) on the relative expression of several genes involved in liver oxidative mechanisms and the oxidative status of the blood and breast muscle of broiler chickens was investigated. In total, 240 one-day-old as hatched chicks (Ross 308) were assigned to four treatments, with four replicate pens and 15 birds in each pen. Birds were fed either a basal diet (CON) or a basal diet supplemented with 25 g/kg GGP, or 2 g/kg WYC, or 1 g starch including 100 mg pure stem extract/kg (PE) for 42 days. The polyphenolic content of vinification by-products was determined using an LC-MS/MS library indicating as prevailing compounds procyanidin B1 and B2, gallic acid, caftaric acid, (+)-catechin, quercetin, and trans-resveratrol. Body weight and feed consumption were not significantly affected. The relative transcript level of GPX1 and SOD1 tended to increase in the liver of WYC-fed broilers, while NOX2 tended to decrease in the PE group. SOD activity in blood plasma was significantly increased in WYC and PE compared to the CON group. The total antioxidant capacity measured with FRAP assay showed significantly higher values in the breast muscle of PE-fed broilers, while the malondialdehyde concentration was significantly decreased in both WYC- and PE-fed broilers compared to the CON group. The exploitation of vinification by-products as feed additives appears to be a promising strategy to improve waste valorization and supply animals with bioactive molecules capable of improving animals’ oxidative status and products’ oxidative stability.
Online Blended Learning in Small Private Online Course
Authors: Han Yong , Wu Wenjun , Zhang Lijun , Liang Yu
Published: August 2021

In this work, we studied the online blended learning model of computer network experimentation, focusing mainly on the problem of traditional network experiments being limited by location and time, and explore the applicability of the small private online course (SPOC) advanced teaching concepts to computer network online experiment teaching. Based on the structure of a combination of virtual and real, real and not virtual, an online network experiment platform and management system has been designed and constructed, enabling students to carry out remote online computer network hardware experiments anytime and anywhere, without being restricted by time, space, or content. Using the online network experiment platform, we can organize the experimental modules and knowledge points via the SPOC course concept, by developing online network experimental content, modularizing and fragmenting of the experiments, creating the pre-experimental explanation and experiment preview videos, and evaluating the assignments via peer grading to analyze students’ learning behavior. By exploring online network experimental teaching methods and management models, offering experimental guidance in an interactive manner, and highlighting the openness and sharing characteristics of online experimental teaching platforms, we can improve the utilization rate for teaching resources, and provide ideas for applied scientific research methods.
Liquid Crystal-Embedded Hollow Core Fiber Temperature Sensor in Fiber Ring Laser
Authors: Lin Weihao , Zhou Shengjie , Liu Yibin , Vai Mang I. , Shao Liyang
Published: August 2021

An optical fiber temperature sensor based on Mach–Zehnder interferometer and thermo-optic effect of the liquid crystal (LC) in fiber ring laser (FRL) system is proposed and experimentally demonstrated. The LC is infiltrated into the core of hollow core fiber, and the resonant wavelength is more sensitive to temperature variation due to the interaction between the incident light and the cavity infiltrating liquid crystal with high thermal light coefficient. Meanwhile, the FRL system was further used to make the sensor have good performance in the case of high signal-to-noise ratio (∼35 dB), narrow half-height width (FWHM = 0.15 nm), and high sensitivity in the temperature range from 20 °C to 50 °C, with the maximum sensitivity of 1.318 nm/°C. As far as we know, in the FRL system, the liquid crystal material has a better temperature sensing performance than the previous fiber. Nevertheless, the system has the advantages of good repeatability, low cost, simple production, small volume, high sensitivity. In marine microbial culture and detection, it is necessary to carry out high sensitivity measurement within a small temperature variation range. This reliable and excellent temperature performance has a potential application prospect.
Probability Characteristics, Area Reduction, and Wind Directionality Effects of Extreme Pressure Coefficients of High-Rise Buildings
Authors: Li Shouke , Xiao Feipeng , Zou Yunfeng , Li Shouying , Yang Shucheng , Feng Chao , Chen Yuankun
Published: August 2021

Wind tunnel tests are carried out for the Commonwealth Advisory Aeronautical Research Council (CAARC) high-rise building with a scale of 1:400 in exposure categories D. The distribution law of extreme pressure coefficients under different conditions is studied. Probability distribution fitting is performed on the measured area-averaged extreme pressure coefficients. The general extreme value (GEV) distribution is preferred for probability distribution fitting of extreme pressure coefficients. From the comparison between the area-averaged coefficients and the value from GB50009-2012, it is indicated that the wind load coefficients from GB50009-2012 may be non-conservative for the CAARC building. The area reduction effect on the extreme wind pressure is smaller than that on the mean wind pressure from the code. The recommended formula of the area reduction factor for the extreme pressure coefficient is proposed in this study. It is found that the mean and the coefficient of variation (COV) for the directionality factors are 0.85 and 0.04, respectively, when the orientation of the building is given. If the uniform distribution is given for the building’s orientation, the mean value of the directionality factors is 0.88, which is close to the directionality factor of 0.90 given in the Chinese specifications.
Spectral Broadening in a Continuously Pumped Singly Resonant Second-Harmonic Cavity
Authors: Mosca Simona , Hansson Tobias , Parisi Maria
Published: August 2021

Optical frequency comb synthesizers with a wide spectral range are an essential tool for many research areas such as spectroscopy, precision metrology, optical communication, and sensing. Recent studies have demonstrated the direct generation of frequency combs, via second-order processes, that are centered on two different spectral regions separated by an octave. Here, we present the capability of optical quadratic frequency combs for broad-bandwidth spectral emission in unexplored regimes. We consider comb formation under phase-matched conditions in a continuous-wave pumped singly resonant second-harmonic cavity, with large intracavity power and control of the detuning over several cavity linewidths. The spectral analysis reveals quite distinctive sidebands that arise far away from the pump, singularly or in a mixed regime together with narrowband frequency combs. Notably, by increasing the input power, the optical frequency lines evolve into widely spaced frequency clusters, and at maximum power, they appear in a wavelength range spanning up to 100 nm. The obtained results demonstrate the capacity of second-order nonlinearities to produce direct comb within a wide range of wavelengths.
Modeling, Design and Optimization of Flexible Mechanical Systems
Authors: Wehrle Erich , Palomba Ilaria , Vidoni Renato
Published: August 2021

Performance, efficiency and economy drive the design of mechanical systems and structures and has led lightweight engineering design to prominence [...]
A Fast Method for Estimating the Emission Factors of Air Pollutants from In-Use Vehicles Fleet
Authors: Lee Seung-Bok , Kim Kyung Hwan , Park Bo-Eun , Bae Gwi-Nam
Published: August 2021

The real-world emission factors of gaseous and particulate air pollutants emitted from in-use vehicles, can be rapidly estimated using monitoring data of their concentration profiles from inside roadway tunnels using a mobile laboratory equipped with fast monitoring instruments. The concentrations of CO2 and particle-bound polycyclic aromatic hydrocarbons (PM-PAHs) and NOx, were observed to increase linearly with traveling distance inside two successive roadway tunnels: the Hongjimun Tunnel and the Jeongneung Tunnel on the Naebu Express Way in Seoul, Korea, except for a small region of decrease. In the decreasing regions, within a few hundred meters of the entrance and before the exit, outside background air with low concentrations of air pollutants was thought to have intruded. From the slopes of the linear regression between distance and concentrations, a fleet-averaged (light-, medium-, and heavy-duty vehicles with 54%, 36%, and 10%, respectively) emission factor of CO2, PM-PAHs, and NOx at an average speed of ~60 km h−1 could be calculated as 197 ± 38 g km−1, 4.2 ± 0.8 × 10−4 g km−1, and 0.530 ± 0.230 g km−1, respectively, which are within the ranges of values reported in the literature. For each tunnel, the emission factors of CO2, PM-PAHs, and NOx estimated on days with higher-than-normal fractions of heavy-duty vehicles, were higher than those on other days. From these results, the new fast method proposed in this study is considered useful for estimating real-world emission factors of air pollutants by using a mobile laboratory as a complementary tool to traditional tunnel studies. This method can be used to rapidly make emission maps at roadway tunnels in mega-cities like Seoul, Korea, for urban air-quality management.
Guided Spatial Transformers for Facial Expression Recognition
Authors: Luna-Jiménez Cristina , Cristóbal-Martín Jorge , Kleinlein Ricardo , Gil-Martín Manuel , Moya José M. , Fernández-Martínez Fernando
Published: August 2021

Spatial Transformer Networks are considered a powerful algorithm to learn the main areas of an image, but still, they could be more efficient by receiving images with embedded expert knowledge. This paper aims to improve the performance of conventional Spatial Transformers when applied to Facial Expression Recognition. Based on the Spatial Transformers’ capacity of spatial manipulation within networks, we propose different extensions to these models where effective attentional regions are captured employing facial landmarks or facial visual saliency maps. This specific attentional information is then hardcoded to guide the Spatial Transformers to learn the spatial transformations that best fit the proposed regions for better recognition results. For this study, we use two datasets: AffectNet and FER-2013. For AffectNet, we achieve a 0.35% point absolute improvement relative to the traditional Spatial Transformer, whereas for FER-2013, our solution gets an increase of 1.49% when models are fine-tuned with the Affectnet pre-trained weights.
Automated Fault Detection and Extraction under Gas Chimneys Using Hybrid Discontinuity Attributes
Authors: Imran Qazi Sohail , Siddiqui Numair A. , Latiff Abdul Halim Abdul , Bashir Yasir , Khan Muhammad , Qureshi Kamran , Al-Masgari Abd Al-Salam , Ahmed Nisar , Jamil Muhammad
Published: August 2021

3D-seismic data have increasingly shifted seismic interpretation work from a horizons-based to a volume-based focus over the past decade. The size of the identification and mapping work has therefore become difficult and requires faster and better tools. Faults, for instance, are one of the most significant features of subsurface geology interpreted from seismic data. Detailed fault interpretation is very important in reservoir characterization and modeling. The conventional manual fault picking is a time-consuming and inefficient process. It becomes more challenging and error-prone when dealing with poor quality seismic data under gas chimneys. Several seismic attributes are available for faults and discontinuity detection and are applied with varying degrees of success. We present a hybrid workflow that combines a semblance-based fault likelihood attribute with a conventional ant-tracking attribute. This innovative workflow generates optimized discontinuity volumes for fault detection and automatic extraction. The data optimization and conditioning processes are applied to suppress random and coherent noise first, and then a combination of seismic attributes is generated and co-rendered to enhance the discontinuities. The result is the volume with razor sharp discontinuities which are tracked and extracted automatically. Contrary to several available fault tracking techniques that use local seismic continuity like coherency attributes, our hybrid method is based on directed semblance, which incorporates aspects of Dave Hale’s superior fault-oriented semblance algorithm. The methodology is applied on a complex faulted reservoir interval under gas chimneys in a Malaysian basin, yet the results were promising. Despite the poor data quality, the methodology led to detailed discontinuity information with several major and minor faults extracted automatically. This hybrid approach not only improved the fault tracking accuracy but also significantly reduced the fault interpretation time and associated uncertainty. It is equally helpful in detecting any seismic objects like fracture, chimneys, and stratigraphic features.
Attack Categorisation for IoT Applications in Critical Infrastructures, a Survey
Authors: Staddon Edward , Loscri Valeria , Mitton Nathalie
Published: August 2021

With the ever advancing expansion of the Internet of Things (IoT) into our everyday lives, the number of attack possibilities increases. Furthermore, with the incorporation of the IoT into Critical Infrastructure (CI) hardware and applications, the protection of not only the systems but the citizens themselves has become paramount. To do so, specialists must be able to gain a foothold in the ongoing cyber attack war-zone. By organising the various attacks against their systems, these specialists can not only gain a quick overview of what they might expect but also gain knowledge into the specifications of the attacks based on the categorisation method used. This paper presents a glimpse into the area of IoT Critical Infrastructure security as well as an overview and analysis of attack categorisation methodologies in the context of wireless IoT-based Critical Infrastructure applications. We believe this can be a guide to aid further researchers in their choice of adapted categorisation approaches. Indeed, adapting appropriated categorisation leads to a quicker attack detection, identification, and recovery. It is, thus, paramount to have a clear vision of the threat landscapes of a specific system.
High Visibility Conditions in a Sunset Environment
Authors: Iizuka Tatsuya , Kawamorita Takushi , Takenaka Choku , Tsuji Hajime , Kanai Hiroyuki , Hirai Toshihiro , Suzuki Hiroo , Handa Tomoya , Ishikawa Hitoshi
Published: August 2021

There is no standard for high-visibility safety clothing for general pedestrians, nor is it widely used. Therefore, this study investigated visibility in order to examine the standards for high-visibility safety clothing for general pedestrians. Methods: Twenty healthy participants (mean age, 22.4 ± 4.4 years) without ocular disease, except for refractive errors, were studied. All participants had healthy visual acuity in corrected vision. This study assumed sunset conditions in Japan. The light source was set up in a dark room, and the illuminance in front of the visual target was set to 300 lx. We investigated the visibility of 142 patterns of black and yellow combination samples with different spatial frequencies, pattern types, angles, and color ratios. Results: The highest visibility was found at 5.0 cycles per degree of the stripe pattern at the spatial frequency, yellow ratio of 75%, and a stripe angle of 165°. Conclusions: Under sunset conditions, the brightness decreased when black was combined with yellow. However, it forms a two-color pattern and becomes more conspicuous. The highest visibility was obtained by arranging black and yellow in a diagonal stripe pattern. We predict that establishing clothing standards based on the results of this study will help design safety clothing with increased visibility.
The Influence of Transport on PAHs and Other Carbonaceous Species’ (OC, EC) Concentration in Aerosols in the Coastal Zone of the Gulf of Gdansk (Gdynia)
Authors: Buch Joanna Klaudia , Lewandowska Anita Urszula , Staniszewska Marta , Wiśniewska Kinga Areta , Bartkowski Karolina Venessa
Published: August 2021

The aim of this study was to determine the influence of transport on the concentration of carbon species in aerosols collected in the coastal zone of the Gulf of Gdansk in the period outside the heating season. Elemental carbon (EC), organic carbon (OC), and the ΣPAHs5 concentrations were measured in aerosols of two size: <3 μm (respirable aerosols) and >3 μm in diameter (inhalable aerosols). Samples were collected between 13 July 2015 and 22 July 2015 (holiday period) and between 14 September 2015 and 30 September 2015 (school period). In both periods samples were taken only during the morning (7:00–9:00 a.m.) and afternoon (3:00–5:00 p.m.) road traffic hours. The highest mean values of the ΣPAHs5 and EC were recorded in small particles during the school period in the morning road traffic peak hours. The mean concentration of OC was the highest in small aerosols during the holiday period. However, there were no statistically significant differences between the concentrations of organic carbon in the morning and afternoon peak hours. Strict sampling and measurement procedures, together with the analysis of air mass backward trajectories and pollutant markers, indicated that the role of land transport was the greatest when local to regional winds prevailed, bringing pollution from nearby schools and the beltway.
Air Quality in the Italian Northwestern Alps during Year 2020: Assessment of the COVID-19 «Lockdown Effect» from Multi-Technique Observations and Models
Authors: Diémoz Henri , Magri Tiziana , Pession Giordano , Tarricone Claudia , Tombolato Ivan Karl Friedrich , Fasano Gabriele , Zublena Manuela
Published: August 2021

The effect of COVID-19 confinement regulations on air quality in the northwestern Alps is assessed here based on measurements at five valley sites in different environmental contexts. Surface concentrations of nitrogen oxides (NO and NO2), ozone (O3), particulate matter (PM2.5 and PM10), together with a thorough microphysical (size), chemical, and optical (light absorption) aerosol characterisation, complemented by observations along the vertical column are considered. Even in the relatively pristine environment of the Alps, the «lockdown effect» is well discernible, both in the early confinement phase and in late 2020. The variations observed during the first confinement period in the city of Aosta (−61% NO, −43% NO2, +5% O3, +9% PM2.5, −12% PM10, relative to average 2015–2019 conditions) are attributed to the competing effects of air pollution lockdown-induced changes (−74%, −52%, +18%, −13%, −27%, relative to the counterfactual scenario for 2020 provided by a predictive statistical model trained on past measurements) and meteorology (+52%, +18%, −11%, +25%, +20%, relative to average conditions). These changes agree well with the ones obtained from a chemical transport model with modified emissions according to the restrictions. With regard to column-integrated quantities and vertical profiles, the NO2 column density decreases by >20% due to the lockdown, whereas tropospheric aerosols are mainly influenced by large-scale dynamics (transport of secondary particles from the Po basin and mineral dust from the Sahara desert and the Caspian Sea), except a shallow layer about 500 m thick close to the surface, possibly sensitive to curtailed emissions (especially exhaust and non-exhaust particles from road traffic and fugitive emissions from the industry).
How Do Mechanics Guide Fibroblast Activity? Complex Disruptions during Emphysema Shape Cellular Responses and Limit Research
Authors: Leslie Mathew N. , Chou Joshua , Young Paul M. , Traini Daniela , Bradbury Peta , Ong Hui Xin
Published: August 2021

The emphysema death toll has steadily risen over recent decades, causing the disease to become the third most common cause of death worldwide in 2019. Emphysema is currently incurable and could be due to a genetic condition (Alpha-1 antitrypsin deficiency) or exposure to pollutants/irritants, such as cigarette smoke or poorly ventilated cooking fires. Despite the growing burden of emphysema, the mechanisms behind emphysematous pathogenesis and progression are not fully understood by the scientific literature. A key aspect of emphysematous progression is the destruction of the lung parenchyma extracellular matrix (ECM), causing a drastic shift in the mechanical properties of the lung (known as mechanobiology). The mechanical properties of the lung such as the stiffness of the parenchyma (measured as the elastic modulus) and the stretch forces required for inhalation and exhalation are both reduced in emphysema. Fibroblasts function to maintain the structural and mechanical integrity of the lung parenchyma, yet, in the context of emphysema, these fibroblasts appear incapable of repairing the ECM, allowing emphysema to progress. This relationship between the disturbances in the mechanical cues experienced by an emphysematous lung and fibroblast behaviour is constantly overlooked and consequently understudied, thus warranting further research. Interestingly, the failure of current research models to integrate the altered mechanical environment of an emphysematous lung may be limiting our understanding of emphysematous pathogenesis and progression, potentially disrupting the development of novel treatments. This review will focus on the significance of emphysematous lung mechanobiology to fibroblast activity and current research limitations by examining: (1) the impact of mechanical cues on fibroblast activity and the cell cycle, (2) the potential role of mechanical cues in the diminished activity of emphysematous fibroblasts and, finally, (3) the limitations of current emphysematous lung research models and treatments as a result of the overlooked emphysematous mechanical environment.
COVID-19 Shuts Doors to Flu but Keeps Them Open to Rhinoviruses
Authors: Kiseleva Irina , Ksenafontov Andrey
Published: August 2021

It is well known that rhinoviruses are distributed across the globe and are the most common cause of the common cold in all age groups. Rhinoviruses are widely considered to be harmless because they are generally perceived as respiratory viruses only capable of causing mild disease. However, they may also infect the lower respiratory tract, inducing chronic obstructive pulmonary disease and exacerbations of asthma, bronchiolitis, etc. The role of rhinoviruses in pathogenesis and the epidemiological process is underestimated, and they need to be intensively studied. In the light of recent data, it is now known that rhinoviruses could be one of the key epidemiological barriers that may influence the spread of influenza and novel coronaviruses. It has been reported that endemic human rhinoviruses delayed the development of the H1N1pdm09 influenza pandemic through viral interference. Moreover, human rhinoviruses have been suggested to block SARS-CoV-2 replication in the airways by triggering an interferon response. In this review, we summarized the main biological characteristics of genetically distinct viruses such as rhinoviruses, influenza viruses, and SARS-CoV-2 in an attempt to illuminate their main discrepancies and similarities. We hope that this comparative analysis will help us to better understand in which direction research in this area should move.
Associations of Vitamin D Levels with Physical Fitness and Motor Performance; A Cross-Sectional Study in Youth Soccer Players from Southern Croatia
Authors: Gilic Barbara , Kosor Jelena , Jimenez-Pavon David , Markic Josko , Karin Zeljka , Domic Daniela Supe , Sekulic Damir
Published: August 2021

Vitamin D level is known to be a factor potentially influencing physical fitness, but few studies have examined this phenomenon among youth athletes. We aimed to evaluate the associations of vitamin D levels (as measured by 25-hydroxyvitamin D concentrations—25(OH)D) with various physical fitness and motor performance tests in youth football (soccer) players. This cross-sectional study included a total of 52 youth soccer players (15.98 ± 2.26 years old) from Southern Croatia. The participants were evaluated at the end of the winter period and data were collected of anthropometric measures (body mass and body height), vitamin D status (25(OH)D levels), physical fitness tests (sprints of 10 and 20 m, 20 yards test, the countermovement jump, the reactive strength index (RSI)) and motor performance tests (the soccer-specific CODS, the soccer-specific agility, and static balance). Among the studied players, 54% had 25(OH)D insufficiency/deficiency, showing a lack of 25(OH)D is widespread even in youth athletes living at a southern latitude. The 25(OH)D level was correlated with sprint 20 m, 20 yards tests, and RSI, showing a greater role of 25(OH)D in physical fitness tests where energetic capacity is essential than in sport-related motor performance tests where skills are crucial. Our results support the idea that vitamin D can play a determinant role in physical fitness tests with a clear physiological component, but is not crucial in motor performance tests related to specific sports where skills are a key component. Future studies should investigate the effects of vitamin D supplementation on the performance in physical fitness and motor performance tests among youth athletes.
Pre-Existing Diabetes and COVID-Associated Hyperglycaemia in Patients with COVID-19 Pneumonia
Authors: Laurenzi Andrea , Caretto Amelia , Molinari Chiara , Bazzigaluppi Elena , Brigatti Cristina , Marzinotto Ilaria , Mercalli Alessia , Melzi Raffaella , Nano Rita , Tresoldi Cristina , Landoni Giovanni , Ciceri Fabio , Lampasona Vito , Scavini Marina , Piemonti Lorenzo
Published: August 2021

Aim. The aim of the current study was to compare clinical characteristics, laboratory findings, and major outcomes of patients hospitalized for COVID-19 pneumonia with COVID-associated hyperglycaemia or pre-existing diabetes. Methods. A cohort of 176 adult patients with a diagnosis of pre-existing diabetes (n = 112) or COVID-associated hyperglycaemia (n = 55) was studied. Results. Patients with COVID-associated hyperglycaemia had lower BMI, significantly less comorbidities, and higher levels of inflammatory markers and indicators of multi-organ injury than those with pre-existing diabetes. No differences between pre-existing diabetes and COVID-associated hyperglycaemia were evident for symptoms at admission, the humoral response against SARS-CoV-2, or autoantibodies to glutamic acid decarboxylase or interferon alpha-4. COVID-associated hyperglycaemia was independently associated with the risk of adverse clinical outcome, which was defined as ICU admission or death (HR 2.11, 95% CI 1.34–3.31; p = 0.001), even after adjustment for age, sex, and other selected variables associated with COVID-19 severity. Furthermore, at the same time, we documented a negative association (HR 0.661, 95% CI 0.43–1.02; p = 0.063) between COVID-associated hyperglycaemia to swab negativization. Conclusions. Recognizing hyperglycaemia as a specific clinical entity associated with COVID-19 pneumonia is relevant for early and appropriate patient management and close monitoring for the progression of disease severity.
TCMacro: A Simple and Robust ImageJ-Based Method for Automated Measurement of Tail Coiling Activity in Zebrafish
Authors: Kurnia Kevin Adi , Santoso Fiorency , Sampurna Bonifasius Putera , Audira Gilbert , Huang Jong-Chin , Chen Kelvin H.-C. , Hsiao Chung-Der
Published: August 2021

Tail coiling is a reflection response in fish embryos that can be used as a model for neurotoxic analysis. The previous method to analyze fish tail coiling is largely based on third-party software. In this study, we aim to develop a simple and cost-effective method called TCMacro by using ImageJ macro to reduce the operational complexity. The basic principle of the current method is based on the dynamic change of pixel intensity in the region of interest (ROI). When the fish tail is moving, the average intensity is increasing. In time when the fish freeze, the peak of mean intensity is maintaining at a relatively low level. By using the optimized macro settings and excel VBA scripts, all the tail coiling measurement processes can be archived with few operation steps with high precision. Three major endpoints of tail coiling counts, tail coiling duration and tail coiling intervals can be obtained in batch. To validate this established method, we tested the potential neurotoxic activity of Tricaine (methanesulfonate, MS-222) and psychoactive compound of caffeine. Zebrafish embryos after Tricaine exposure displayed significantly less tail coiling activity in a dose-dependent manner, and were comparable to manual counting through the Wilcoxon test and Pearson correlation double validation. Zebrafish embryos after caffeine exposure displayed significantly high tail coiling activity. In conclusion, the TCMacro method presented in this study provides a simple and robust method that is able to measure the relative tail coiling activities in zebrafish embryos in a high-throughput manner.
Diagnostic Significance of Serum Galectin-3 in Hospitalized Patients with COVID-19—A Preliminary Study
Authors: Kuśnierz-Cabala Beata , Maziarz Barbara , Dumnicka Paulina , Dembiński Marcin , Kapusta Maria , Bociąga-Jasik Monika , Winiarski Marek , Garlicki Aleksander , Grodzicki Tomasz , Kukla Michał
Published: August 2021

Severe coronavirus disease 2019 (COVID-19) is associated with hyperinflammation leading to organ injury, including respiratory failure. Galectin-3 was implicated in innate immunological response to infections and in chronic fibrosis. The aim of our preliminary study was the assessment of the diagnostic utility of serum galectin-3 in patients with COVID-19. The prospective observational study included adult patients admitted with active COVID-19 and treated in tertiary hospital between June and July 2020. The diagnosis was confirmed by the quantitative detection of nucleic acid of severe acute respiratory syndrome coronavirus 2 in nasopharyngeal swabs. Galectin-3 was measured by enzyme immunoassay in serum samples obtained during the first five days of hospital stay. We included 70 patients aged 25 to 73 years; 90% had at least one comorbidity. During the hospital stay, 32.9% were diagnosed with COVID-19 pneumonia and 12.9% required treatment in the intensive care unit (ICU). Serum galectin-3 was significantly increased in patients who developed pneumonia, particularly those who required ICU admission. Positive correlations were found between galectin-3 and inflammatory markers (interleukin-6, C-reactive protein, ferritin, pentraxin-3), a marker of endothelial injury (soluble fms-like tyrosine kinase-1), and a range of tissue injury markers. Serum galectin-3 enabled the diagnosis of pneumonia with moderate diagnostic accuracy and the need for ICU treatment with high diagnostic accuracy. Our findings strengthen the hypothesis that galectin-3 may be involved in severe COVID-19. Further studies are planned to confirm the preliminary results and to verify possible associations of galectin-3 with long-term consequences of COVID-19, including pulmonary fibrosis.
Novel Glutamate–Putrescine Ligase Activity in Haloferax mediterranei: A New Function for glnA-2 Gene
Authors: Rodríguez-Herrero Verónica , Peris Arnau , Camacho Mónica , Bautista Vanesa , Esclapez Julia , Bonete María-José
Published: August 2021

The genome of the halophilic archaea Haloferax mediterranei contains three ORFs that show homology with glutamine synthetase (GS) (glnA-1, glnA-2, and glnA-3). Previous studies have focused on the role of GlnA-1, suggesting that proteins GlnA-2 and GlnA-3 could play a different role to that of GS. Glutamine synthetase (EC 6.3.1.2) belongs to the class of ligases, including 20 subclasses of other different enzymes, such as aspartate–ammonia ligase (EC 6.3.1.1), glutamate–ethylamine ligase (EC 6.3.1.6), and glutamate–putrescine ligase (EC 6.3.1.11). The reaction catalyzed by glutamate–putrescine ligase is comparable to the reaction catalyzed by glutamine synthetase (GS). Both enzymes can bind a glutamate molecule to an amino group: ammonium (GS) or putrescine (glutamate–putrescine ligase). In addition, they present the characteristic catalytic domain of GS, showing significant similarities in their structure. Although these proteins are annotated as GS, the bioinformatics and experimental results obtained in this work indicate that the GlnA-2 protein (HFX_1688) is a glutamate–putrescine ligase, involved in polyamine catabolism. The most significant results are those related to glutamate–putrescine ligase’s activity and the analysis of the transcriptional and translational expression of the glnA-2 gene in the presence of different nitrogen sources. This work confirms a new metabolic pathway in the Archaea domain which extends the knowledge regarding the utilization of alternative nitrogen sources in this domain.
Strontium Binding to α-Parvalbumin, a Canonical Calcium-Binding Protein of the “EF-Hand” Family
Authors: Vologzhannikova Alisa A. , Shevelyova Marina P. , Kazakov Alexey S. , Sokolov Andrey S. , Borisova Nadezhda I. , Permyakov Eugene A. , Kircheva Nikoleta , Nikolova Valya , Dudev Todor , Permyakov Sergei E.
Published: August 2021

Strontium salts are used for treatment of osteoporosis and bone cancer, but their impact on calcium-mediated physiological processes remains obscure. To explore Sr2+ interference with Ca2+ binding to proteins of the EF-hand family, we studied Sr2+/Ca2+ interaction with a canonical EF-hand protein, α-parvalbumin (α-PA). Evaluation of the equilibrium metal association constants for the active Ca2+ binding sites of recombinant human α-PA (‘CD’ and ‘EF’ sites) from fluorimetric titration experiments and isothermal titration calorimetry data gave 4 × 109 M−1 and 4 × 109 M−1 for Ca2+, and 2 × 107 M−1 and 2 × 106 M−1 for Sr2+. Inactivation of the EF site by homologous substitution of the Ca2+-coordinating Glu in position 12 of the EF-loop by Gln decreased Ca2+/Sr2+ affinity of the protein by an order of magnitude, whereas the analogous inactivation of the CD site induced much deeper suppression of the Ca2+/Sr2+ affinity. These results suggest that Sr2+ and Ca2+ bind to CD/EF sites of α-PA and the Ca2+/Sr2+ binding are sequential processes with the CD site being occupied first. Spectrofluorimetric Sr2+ titration of the Ca2+-loaded α-PA revealed presence of secondary Sr2+ binding site(s) with an apparent equilibrium association constant of 4 × 105 M−1. Fourier-transform infrared spectroscopy data evidence that Ca2+/Sr2+-loaded forms of α-PA exhibit similar states of their COO− groups. Near-UV circular dichroism (CD) data show that Ca2+/Sr2+ binding to α-PA induce similar changes in symmetry of microenvironment of its Phe residues. Far-UV CD experiments reveal that Ca2+/Sr2+ binding are accompanied by nearly identical changes in secondary structure of α-PA. Meanwhile, scanning calorimetry measurements show markedly lower Sr2+-induced increase in stability of tertiary structure of α-PA, compared to the Ca2+-induced effect. Theoretical modeling using Density Functional Theory computations with Polarizable Continuum Model calculations confirms that Ca2+-binding sites of α-PA are well protected against exchange of Ca2+ for Sr2+ regardless of coordination number of Sr2+, solvent exposure or rigidity of sites. The latter appears to be a key determinant of the Ca2+/Sr2+ selectivity. Overall, despite lowered affinity of α-PA to Sr2+, the latter competes with Ca2+ for the same EF-hands and induces similar structural rearrangements. The presence of a secondary Sr2+ binding site(s) could be a factor contributing to Sr2+ impact on the functional activity of proteins.