bluuebunny/crossref_metadata_2025 huggingface dataset:
Injury-Inciting Activities in Male and Female Football Players: A Systematic Review
Authors: Aiello Francesco , Impellizzeri Franco M. , Brown Susan J. , Serner Andreas , McCall Alan
Published: October 2022

Background

A comprehensive examination of the sport-specific activities performed around the time of injury is important to hypothesise injury mechanisms, develop prevention strategies, improve management, and inform future investigations. The aim of this systematic review is to summarise the current literature describing the activities performed around the time of injury in football (soccer).

Methods

A systematic search was carried out in PubMed, Web of Science, SPORTDiscus, and OpenGrey. Studies were included if participants were football players aged > 13 years old and the activities performed at the time of injury were reported together with the total number of injuries. Risk of bias was assessed using an adapted version of checklists developed for prevalence studies. The activities reported by the studies were grouped to account for inconsistent reporting, and the proportion of each injury activity was calculated. Data were not meta-analysed due to high heterogeneity of methods and classification criteria.

Results

We included 64 studies reporting on 56,740 injuries in total. ACL injures were analysed by 12 studies, ankle/foot and knee injuries were analysed by five studies, thigh injuries were analysed by four studies, hip/groin injuries were analysed by three studies, and hamstring injuries were analysed by two studies. Five studies analysed more than one type of injury and 38 studies did not specify the type of injuries analysed. Running and kicking were the predominant activities leading to thigh and hamstring injuries. Changing direction and kicking were the predominant activities leading to hip and groin injuries and duels were the predominant activities leading to ankle injuries. Duels and pressing seem the predominant activities leading to ACL injuries, while results for other knee and general injuries were inconsistent.

Conclusions

A qualitative summary of the activities performed at the time of injury has been reported. The results need to be interpreted carefully due to the risk of bias observed in the included studies. If we are to meaningfully progress our knowledge in this area, it is paramount that future research uses consistent methods to record and classify injuries and activities leading up to and performed at the time of injury.

Registration

The protocol of this systematic review was registered at the Open Science Framework (https://doi.org/10.17605/OSF.IO/U96KV).
Minimal Intervention Dentistry (MID) mainstream or unconventional option? Study exploring the impact of COVID-19 on paediatric dentists’ views and practices of MID for managing carious primary teeth in children across the United Kingdom and European Union
Authors: BaniHani A. , Hamid A. , Van Eeckhoven J. , Gizani S. , Albadri S.
Published: October 2022

Purpose

To explore the techniques used to manage carious primary teeth during the COVID-19 pandemic by paediatric dentists and dentists with a special interest in paediatric dentistry (DwSI) who are members of the British Society of Paediatric Dentistry (BSPD) and the European Academy of Paediatric Dentistry (EAPD), and their views on the use of minimal intervention dentistry (MID) in children prior to, during and post the COVID era.

Methods

A total of 212 paediatric dentists and DwSI completed an online questionnaire. Six MID techniques were explored: fissure sealants, resin infiltration, Hall Technique (HT), 38% silver diamine fluoride (SDF), atraumatic restorative treatment (ART), stepwise removal and selective caries removal.

Results

The majority were specialists (26%) followed by clinical academics (23.1%) working mainly in university teaching hospitals (46.2%). Routine dental treatment for children with carious primary teeth was provided by the majority (92.5%) during the pandemic. HT (96%) and 38% SDF (65.7%) were the most commonly used techniques among the BSPD members whereas conventional restoration of non-selective caries removal and pulp therapy remained the most widely used technique among the EAPD members (66.2%). Most of the MID techniques were used as a treatment option (48.1%) rather than a choice (43.4%), with most of these choices having been affected by the patient’s behaviour (82.5%). More than one thirds (39.2%) of the participants were reluctant to adopt MID after the pandemic. Several barriers such as lack of teaching and confidence as well as perceived lack of evidence were identified.

Conclusion

A range of MID techniques is practiced broadly by a sample of paediatric dentists and DwSI across the United Kingdom (U.K) and European Union (E.U). The majority of clinicians are willing to continue using these techniques going forward after COVID restrictions are lifted. The pandemic served as an opportunity for many dentists to become familiar with various MID practices, such as SDF, which has been already established some time ago.
Quasi-Solid-State Ion-Conducting Arrays Composite Electrolytes with Fast Ion Transport Vertical-Aligned Interfaces for All-Weather Practical Lithium-Metal Batteries
Authors: Li Xinyang , Wang Yong , Xi Kai , Yu Wei , Feng Jie , Gao Guoxin , Wu Hu , Jiang Qiu , Abdelkader Amr , Hua Weibo , Zhong Guiming , Ding Shujiang
Published: October 2022

The rapid improvement in the gel polymer electrolytes (GPEs) with high ionic conductivity brought it closer to practical applications in solid-state Li-metal batteries. The combination of solvent and polymer enables quasi-liquid fast ion transport in the GPEs. However, different ion transport capacity between solvent and polymer will cause local nonuniform Li+ distribution, leading to severe dendrite growth. In addition, the poor thermal stability of the solvent also limits the operating-temperature window of the electrolytes. Optimizing the ion transport environment and enhancing the thermal stability are two major challenges that hinder the application of GPEs. Here, a strategy by introducing ion-conducting arrays (ICA) is created by vertical-aligned montmorillonite into GPE. Rapid ion transport on the ICA was demonstrated by 6Li solid-state nuclear magnetic resonance and synchrotron X-ray diffraction, combined with computer simulations to visualize the transport process. Compared with conventional randomly dispersed fillers, ICA provides continuous interfaces to regulate the ion transport environment and enhances the tolerance of GPEs to extreme temperatures. Therefore, GPE/ICA exhibits high room-temperature ionic conductivity (1.08 mS cm−1) and long-term stable Li deposition/stripping cycles (> 1000 h). As a final proof, Li||GPE/ICA||LiFePO4 cells exhibit excellent cycle performance at wide temperature range (from 0 to 60 °C), which shows a promising path toward all-weather practical solid-state batteries.
Nanocrystalline Iron Pyrophosphate-Regulated Amorphous Phosphate Overlayer for Enhancing Solar Water Oxidation
Authors: Xia Chengkai , Li Yuankai , Je Minyeong , Kim Jaekyum , Cho Sung Min , Choi Chang Hyuck , Choi Heechae , Kim Tae-Hoon , Kim Jung Kyu
Published: October 2022

A rational regulation of the solar water splitting reaction pathway by adjusting the surface composition and phase structure of catalysts is a substantial approach to ameliorate the sluggish reaction kinetics and improve the energy conversion efficiency. In this study, we demonstrate a nanocrystalline iron pyrophosphate (Fe4(P2O7)3, FePy)-regulated hybrid overlayer with amorphous iron phosphate (FePO4, FePi) on the surface of metal oxide nanostructure with boosted photoelectrochemical (PEC) water oxidation. By manipulating the facile electrochemical surface treatment followed by the phosphating process, nanocrystalline FePy is localized in the FePi amorphous overlayer to form a heterogeneous hybrid structure. The FePy-regulated hybrid overlayer (FePy@FePi) results in significantly enhanced PEC performance with long-term durability. Compared with the homogeneous FePi amorphous overlayer, FePy@FePi can improve the charge transfer efficiency more significantly, from 60% of FePi to 79% of FePy@FePi. Our density-functional theory calculations reveal that the coexistence of FePi and FePy phases on the surface of metal oxide results in much better oxygen evolution reaction kinetics, where the FePi was found to have a typical down-hill reaction for the conversion from OH* to O2, while FePy has a low free energy for the formation of OH*.

Graphical abstract
The Effects of Teaching Undergraduate Freshmen Biology Courses in Spanish and English
Authors: Chapman Angela , Weimer Amy A. , Torres-Avila Mirayda , Trejo Cristina , Racelis Alexis
Published: October 2022

This study examines the attitudes and experiences of bilingual learners across two instructional types, identifying how the language of instruction affects the context of learning, and how the experience of the learners differs. Participants included 269 predominantly Hispanic undergraduate students in a general biology course attending a Hispanic Serving Institution in a bilingual community along the US-Mexico border. A bilingual faculty member, who recognizes the sociocultural and contextual factors that affect student learning and adopts a teaching philosophy founded in Culturally Responsive Practices (CRP) and valuing students’ Funds of Knowledge taught the class. This study utilized a mixed method design including analyses of quantitative student learning outcomesa and qualitative data on students’ experiences in educational contexts. While all learners were enrolled in sections of the course in which the instructor used CRP, only half of the learners received instruction bilingually in Spanish and English, allowing a comparison of the effects of bilingual instruction. Analyses of student experiences and knowledge gained revealed significantly greater learning in the bilingual courses, compared to English monolingual sections taught by the same faculty member. Results suggest that the use of bilingual instructional approaches can lead to positive outcomes for bilingual undergraduate students. These findings can be used by educators and policy makers alike, with interest in promoting the academic and sociocultural development of bilingual undergraduate students.
Breaking bad news in the era of artificial intelligence and algorithmic medicine: an exploration of disclosure and its ethical justification using the hedonic calculus
Authors: Post Benjamin , Badea Cosmin , Faisal Aldo , Brett Stephen J.
Published: October 2022

An appropriate ethical framework around the use of Artificial Intelligence (AI) in healthcare has become a key desirable with the increasingly widespread deployment of this technology. Advances in AI hold the promise of improving the precision of outcome prediction at the level of the individual. However, the addition of these technologies to patient–clinician interactions, as with any complex human interaction, has potential pitfalls. While physicians have always had to carefully consider the ethical background and implications of their actions, detailed deliberations around fast-moving technological progress may not have kept up. We use a common but key challenge in healthcare interactions, the disclosure of bad news (likely imminent death), to illustrate how the philosophical framework of the 'Felicific Calculus' developed in the eighteenth century by Jeremy Bentham, may have a timely quasi-quantitative application in the age of AI. We show how this ethical algorithm can be used to assess, across seven mutually exclusive and exhaustive domains, whether an AI-supported action can be morally justified.
Impact of Spirulina platensis on physicochemical, antioxidant, microbiological and sensory properties of functional labneh
Authors: Ebid Warda M. A. , Ali Gehad S. , Elewa Neimat A. H.
Published: October 2022

Spirulina powder contains vitamins A, E and all B vitamins, high-quality proteins, 18 of the 20 known amino acids, Ca and K as well as many essential minerals and enzymes. The current study was conducted to make healthy Spirulina probiotic labneh (SPL) by investigating the effect of adding Spirulina (Spirulina platensis) powder on physicochemical, microbiological properties, antioxidant activity and sensorial characteristics beside nutritional value compared to control. SPL was inoculated by Lactobacillus acidophilus and Streptococcus thermophilus. Added of microalgae to concentrated fermented buffalo’s milk by (0, 0.2, 0.4, 0.7 and 1% w/v) Spirulina individually. Spirulina probiotic labneh samples exhibited significantly (p ≤ 0.05) increased the viability of probiotic, higher levels of protein, dietary fiber and antioxidant activity, while lower syneresis than the control labneh. Vitamins B1, B9, and B12, as well as minerals like Fe, Zn, K, and Mg, were found to be higher in the SPL samples than in the control. The phytopigments increased with increasing levels of Spirulina with values ranging from 0.16 to 0.61 for chlorophyll a and from 3.10 to 4.89 for Phycocyanin. (Chlorophyll a, carotenoids) were increased with increasing the added levels from Spirulina comparing with control. Subsequently, it is recommended manufacture probiotic labneh with forficate by Spirulina as a potential source for phenolic and flavonoid compounds, phytopigments, vitamins, fiber and a high content of minerals.
Counter-peace: From isolated blockages in peace processes to systemic patterns
Authors: Pogodda Sandra , Richmond Oliver P. , Visoka Gëzim
Published: October 2022

In the face of the current decline or spectacular collapse of peace processes, this article investigates whether peace has become systematically blocked. It investigates whether the ineffectiveness of an ‘international peace architecture’ (IPA) can be explained by a more potent counterpeace system, which is growing in its shadow. It identifies counterpeace as proto-systemic processes that connect spoilers across all scales (local, regional, national, transnational), while exploiting structural blockages to peace and unintended consequences of peace interventions. It elaborates three distinct patterns of blockages to peace in contemporary conflicts across the globe: the stalemate, limited counterpeace, and unmitigated counterpeace. Drawing on the counterrevolution literature, this research asks: Have peace interventions become the source of their own undoing? Which factors consolidate or aggravate emerging conflict patterns? Are blockages to peace systemic enough to construct a sedimentary and layered counterpeace edifice?
Missing from parish records: Anglican and nonconformist occupational differences and the economy of Wales c.1817
Authors: Richardson Frances
Published: October 2022

Recent studies of male occupations have used Anglican baptisms as a source. However, in areas where nonconformity was strong, a significant proportion of baptisms were missing from parish registers: in Wales, around a quarter of births were to Nonconformist fathers in the years 1813–1820. This study analyses whether there were significant differences between the occupations of Anglican and nonconformist fathers based on a 14 per cent sample of Welsh baptisms. Revised estimates suggest that Anglican data underestimate employment in mining and industry. These estimates are used to give an overview of the Welsh economy c.1817.
Age as a yardstick for political citizenship Voting age and eligibility age in Sweden during the twentieth century
Authors: Sandin Bengt , Josefsson Jonathan
Published: October 2022

In this article we analyse the changes in the age of voting and eligibility for office in Sweden during the twentieth century. We scrutinise arguments, actors, and contexts. Age proved to be an important yardstick for political citizenship and a source of political conflicts of importance for the development of democratic institutions which is largely neglected in earlier research on universal suffrage. Democratisation processes not only have led to the inclusion of new groups of citizens, but also exclusions. Our study demonstrates the importance of shifting understanding of young people, family formation, demographic shifts, intergenerational power balances and constitutional dilemmas.
Protecting the Colony from its People: Bushranging, Vagrancy, and Social Control in Colonial New South Wales
Authors: Foster Meg
Published: October 2022

During the 1830s, the Bushranging Act and the Vagrancy Act were crafted to prevent crime, revolt and insurrection in the colony of New South Wales. These statutes contained exceptional methods to police and control colonial populations and suspended legal safeguards designed to protect the population from abuses of power. Supporters of the laws argued that extreme measures were necessary due to the emergency of the occasion. Understanding the Bushranging Act and the Vagrancy Act’s enactment and operation, as well as the purposes they were designed to serve and the liberties they infringed to achieve these ends requires attention to local circumstance. A fine-grained analysis, rooted in the peculiarities of life in colonial New South Wales and anchored by the law’s operation on the ground is needed to understand the malleability of British law at this place and at this time. In this article, I argue that rather than a select criminal contingent, the New South Wales’ authorities increasingly feared that the composition of the colony threatened their colonial enterprise. The Bushranging Act of 1830 and the Vagrancy Act of 1835 contained wide coercive and discretionary powers to mitigate the extent of this threat.
The recently discovered Principe Scops-owl is highly threatened: distribution, habitat associations, and population estimates
Authors: Freitas Bárbara , Melo Martim , do Bom Jesus Ceciliano , da Costa Sátiro R. , dos Santos Yodiney , Crottini Angelica , de Lima Ricardo Faustino
Published: October 2022

Species baseline information is required for effective biodiversity conservation. Here we provide sound ecological data to support effective conservation of the Principe Scops-owl,OtusbikegilaMelo, Freitas, Verbelen, Costa, Pereira, Fuchs, Sangster, Correia, de Lima & Crottini 2022, discovered in 2016. We mapped the observed and potential distribution, inferred habitat associations, estimated population size, and assessed the International Union for Conservation of Nature (IUCN) Red List category for this species. Surveys were carried out across Príncipe Island during the long and short dry seasons, recording owl presence in sampling points along transects. These data were used to model distribution, revealing that the Principe Scops-owl is restricted to 34.4 km2in the south of the island, inside the Príncipe Obô Natural Park. Most records were in lower altitude native forest. Remoteness was the most important variable to explain presence at island level, followed by land use, which showed that the species is restricted to forest, clearly depending on native forest. Distance sampling using different models suggested a population density ranging between 33.4 individuals/km2(95% CI: 23.6–47.2) and 46.4 individuals/km2(95% CI: 29.3–73.6), which extrapolates to an estimated population size ranging from 1,149 individuals (95% CI: 813–1,623) to 1,597 individuals (95% CI: 1,007–2,533). We propose that the species is classified as “Critically Endangered” due to the small extent of occurrence, coupled with occurrence in a single location and inferred continuing declines in the extent of occurrence, area of occupancy, number of mature individuals and area, extent, and quality of habitat. Effective conservation of the Principe Scops-owl requires regular monitoring and further studies focusing on reproduction and potential nest predation by introduced mammals. Widespread support for the conservation of the Natural Park is vital to ensure the protection of this species and the endemic-rich native forests of Príncipe on which it depends.
Multi Grain‐Size Total Sediment Load Model Based on the Disequilibrium Length
Authors: Le Minor Marine , Davy Philippe , Howarth Jamie , Lague Dimitri
Published: October 2022

In natural rivers, sediment heterogeneity and flow variability control the diversity of transport modes that occur. Although these different modes contribute to the total sediment transport, a law extending from bed load to suspended load that is, relevant for a wide range of sediment mixtures and flow conditions is lacking. Besides, a transport‐limited assumption is often made in modeling of fluvial morphodynamics and thus potentially misses under‐/over‐capacity regimes associated with a particular range of grain sizes and hydraulic conditions. We present a Multi Grain‐Size Total Load model based on widely accepted concepts of sediment transport and developed within the transport length framework in combination with an erosion‐deposition formulation. The new transport length model captures the diversity of transport modes as a physical continuum. Transport capacities for single or bimodal grain sizes are reasonably predicted when compared to published data and scale with the bed shear stress through a continuously varying exponent linked to the characteristic transport height. Modeled transport lengths extend over several orders of magnitude at given flow conditions. Extremely long distances suggest that suspended transport is probably never at capacity. The model can be extended to populations of various grain sizes with a threshold of motion corrected from hiding‐exposure. However, further experimental constraints are needed to better describe entrainment and saltation in strongly heterogeneous bed load transport. The new theoretical formalism we introduce paves the way for a Multi Grain‐Size Total Load Sediment Transport model that includes the variety of transport modes in both non‐stationary and stationary regimes.
The Two‐Dimensional and Three‐Dimensional Structures Concerning the Traveling Ionospheric Disturbances Over Japan Caused by Typhoon Faxai
Authors: Song Rui , Hattori Katsumi , Zhang Xuemin , Liu Jann‐Yenq
Published: October 2022

The two‐dimensional concentric traveling ionospheric disturbances (TIDs) over Japan on the landfall day of Typhoon Faxai in 2019 were detected in total electron content by the ground‐based Global Navigation Satellite System observations. They propagated in the radial direction away from the typhoon eye with a horizontal velocity of ∼190 m/s, a period of ∼24 min, and a horizontal wavelength of ∼180 km. Then, we systematically investigated the corresponding three‐dimensional ionospheric responses for the first time. Remarkable wavelike structures of ionospheric electron density (Ne) and inverted conic‐like structures concerning the disturbed Ne (dNe) were visualized in the F region. Furthermore, the descending phase progression of dNe with increasing time was detected at 220–550 km altitudes, which powerfully revealed that the typhoon‐related TIDs were dominated by the upward atmospheric gravity waves (AGWs) propagating from the troposphere into the ionospheric F2 layer. By analyzing the spatial and temporal evolutions of Ne and dNe, we postulated that the transport process of neutral wind fluctuations associated with the upward AGWs were likely responsible for generating TIDs during Typhoon Faxai. In the end, the background wind velocity was estimated based on the recovered vertical wavelength of ∼460 km and the dispersion equation of AGWs. Our results also demonstrated that the weak background wind condition is essential for gravity waves propagating in the ionosphere.
Thioredoxin-interacting protein deficiency protects against severe acute pancreatitis by suppressing apoptosis signal-regulating kinase 1
Authors: Liu Yanna , Li Mengke , Mei Chaopeng , Cui Huning , Wang Qiaofang , Li Dejian , Song Yaodong , Cui Mengwei , He Qianqian , Chen Sanyang , Zhu Changju
Published: October 2022

Acute pancreatitis is a common acute inflammatory abdominal disease. When acute pancreatitis progresses to severe acute pancreatitis (SAP), it can lead to systemic inflammation and even multiple organ failure. Thioredoxin-interacting protein (TXNIP) is an important protein involved in redox reactions of the inflammatory response. However, the specific role of TXNIP in SAP remains unclear. In this study, we investigated the role of thioredoxin interacting protein (TXNIP) in acute pancreatitis when induced by high doses of arginine. We found that pancreatic damage and the inflammatory response associated with acute pancreatitis were largely restrained in TXNIP knock-out mice but were enhanced in mice overexpressing TXNIP. Interestingly, the phosphorylation of p38, JNK, and ASK1 diminished in TXNIP-KO mice with pancreatitis in comparison with wild-type mice. The role of oxidative stress in SAP was explored in two models: TXNIP and AVV-TXNIP. TXNIP knockdown or the inhibition of ASK1 by gs-4997 abrogated the increase in p-p38, p-JNK, and p-ASK1 in AR42J cells incubated with L-Arg. The administration of gs-4997 to mice with pancreatitis largely reduced the upregulation of IL-6, IL-1β, TNF-α, and MCP-1. Systemic inflammatory reactions and injury in the lungs and kidneys were assessed in TXNIP-KO and AVV-TXNIP mice with expected outcomes. In conclusion, TXNIP is a novel mediator of SAP and exerts action by regulating inflammatory responses and oxidative stress via the ASK1-dependent activation of the JNK/p38 pathways. Thus, targeting TXNIP may represent a promising approach to protect against SAP.
Role of reactive oxygen species in regulating 27-hydroxycholesterol-induced apoptosis of hematopoietic progenitor cells and myeloid cell lines
Authors: Woo Soo-Yeon , Lee Hansong , Park Su Min , Choi Hee-Seon , Kim Jayoung , Kwon Munju , Sohn Jihyung , Nam Ji Ho , Kim Hyung-Sik , Song Parkyong , Baryawno Ninib , Kim Yun-Hak , Kim Koanhoi , Lee Dongjun
Published: October 2022

Oxysterols are oxygenated derivatives of cholesterol that contain an additional hydroxy, epoxide, or ketone group in the sterol nucleus and/or a hydroxyl group in the side chain of the cholesterol molecule. 27-Hydroxycholesterol (27HC) is a side-chain oxysterol that is oxygenated at the 27th carbon atom of cholesterol. The oxysterol (27HC) is produced via oxidation by sterol 27-hydroxylase (CYP27A1) and metabolized via oxysterol 7a-hydroxylase (CYP7B1) for bile acid synthesis in the liver. A previous study has demonstrated that treatment with the alternative Estrogen receptor alpha (ERα) ligand 27HC induces ERα-dependent hematopoietic stem cell (HSC) mobilization. In addition,Cyp27a1-deficient mice demonstrate significantly reduced 27HC levels and HSC mobilization. Here, we report that exogenous 27HC treatment leads to a substantial reduction in the hematopoietic stem and progenitor cell (HSPC) population owing to significantly increased reactive oxygen species (ROS) levels and apoptosis in the bone marrow (BM). However, 27HC does not influence the population of mature hematopoietic cells in the BM. Furthermore, exogenous 27HC treatment suppresses cell growth and promotes ROS production and apoptosis in leukemic cells. Moreover, acute myeloid leukemia (AML) patients with highCYP7B1expression (expected to have inhibition of 27HC) had significantly shorter survival than those with lowCYP7B1expression (expected to have an elevation of 27HC). Single-cell RNA-sequencing (scRNA seq) analysis revealed that the expression ofCYP7B1was significantly increased in AML patients. Thus, our study suggests that 27HC may serve as a potent agent for regulating pools of HSPCs and may have an application as a novel therapeutic target for hematological malignancies. Collectively, pharmacological inhibition of CYP7B1 (expected to have an elevation of 27HC) would potentially have fewer long-term hematological side effects, particularly when used in combination with chemotherapy or radiation for the treatment of leukemia patients.
NOP53 undergoes liquid-liquid phase separation and promotes tumor radio-resistance
Authors: Shi Jie , Chen Si-Ying , Shen Xiao-Ting , Yin Xin-Ke , Zhao Wan-Wen , Bai Shao-Mei , Feng Wei-Xing , Feng Li-Li , Qin Caolitao , Zheng Jian , Wang Yun-Long , Fan Xin-Juan
Published: October 2022

Aberrant DNA damage response (DDR) axis remains the major molecular mechanism for tumor radio-resistance. We recently characterized liquid-liquid phase separation (LLPS) as an essential mechanism of DDR, and identified several key DDR factors as potential LLPS proteins, including nucleolar protein NOP53. In this study, we found that NOP53 formed highly concentrated droplets in vivo and in vitro, which had liquid-like properties including the fusion of adjacent condensates, rapid fluorescence recovery after photobleaching and the sensitivity to 1,6-hexanediol. Moreover, the intrinsically disordered region 1 (IDR1) is required for NOP53 phase separation. In addition, multivalent-arginine-rich linear motifs (M-R motifs), which are enriched in NOP53, were essential for its nucleolar localization, but were dispensable for the LLPS of NOP53. Functionally, NOP53 silencing diminished tumor cell growth, and significantly sensitized colorectal cancer (CRC) cells to radiotherapy. Mechanically, NOP53 negatively regulated p53 pathway in CRC cells treated with or without radiation. Importantly, data from clinical samples confirmed a correlation between NOP53 expression and tumor radio-resistance. Together, these results indicate an important role of NOP53 in radio-resistance, and provide a potential target for tumor radio-sensitization.
Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis
Authors: Kang Fei , Xie Li , Qin Tairan , Miao Yifan , Kang Youhou , Takahashi Toshimasa , Liang Tao , Xie Huanli , Gaisano Herbert Y.
Published: October 2022

Enhancing pancreatic β-cell secretion is a primary therapeutic target for type-2 diabetes (T2D). Syntaxin-2 (Stx2) has just been identified to be an inhibitory SNARE for insulin granule exocytosis, holding potential as a treatment for T2D, yet its molecular underpinnings remain unclear. We show that excessive Stx2 recruitment to raft-like granule docking sites at higher binding affinity than pro-fusion syntaxin-1A effectively competes for and inhibits fusogenic SNARE machineries. Depletion of Stx2 in human β-cells improves insulin secretion by enhancing trans-SNARE complex assembly and cis-SNARE disassembly. Using a genetically-encoded reporter, glucose stimulation is shown to induce Stx2 flipping across the plasma membrane, which relieves its suppression of cytoplasmic fusogenic SNARE complexes to promote insulin secretion. Targeting the flipping efficiency of Stx2 profoundly modulates secretion, which could restore the impaired insulin secretion in diabetes. Here, we show that Stx2 acts to assist this precise tuning of insulin secretion in β-cells, including in diabetes.
A hierarchical transcription factor cascade regulates enteroendocrine cell diversity and plasticity in Drosophila
Authors: Guo Xingting , Zhang Yongchao , Huang Huanwei , Xi Rongwen
Published: October 2022

Enteroendocrine cells (EEs) represent a heterogeneous cell population in intestine and exert endocrine functions by secreting a diverse array of neuropeptides. Although many transcription factors (TFs) required for specification of EEs have been identified in both mammals andDrosophila, it is not understood how these TFs work together to generate this considerable subtype diversity. Here we show that EE diversity in adultDrosophilais generated via an “additive hierarchical TF cascade”. Specifically, a combination of a master TF, a secondary-level TF and a tertiary-level TF constitute a “TF code” for generating EE diversity. We also discover a high degree of post-specification plasticity of EEs, as changes in the code—including as few as one distinct TF—allow efficient switching of subtype identities. Our study thus reveals a hierarchically-organized TF code that underlies EE diversity and plasticity inDrosophila, which can guide investigations of EEs in mammals and inform their application in medicine.
A systematic review and meta-analysis on effects of aerobic exercise in people with Parkinson’s disease
Authors: Zhen Kai , Zhang Shiyan , Tao Xifeng , Li Gen , Lv Yuanyuan , Yu Laikang
Published: October 2022

Previous studies have shown that aerobic exercise is an effective way to improve symptoms of Parkinson’s disease (PD). The aim of this study [PROSPERO CRD42022340730] was to explore the effects of aerobic exercises on balance, gait, motor function, and quality of life in PD patients. Searches were performed in PubMed, Web of Science, and EBSCO electronic databases. The Cochrane risk assessment tool was used to evaluate the methodological quality of the included literature. From 1287 search records initially identified, 20 studies were considered eligible for systematic review and meta-analysis. There was a significant effect of aerobic exercise on improving timed up and go test [standardized mean difference (SMD), −0.41 (95% CI, −0.61 to −0.22), p < 0.00001], Berg Balance Scale [0.99 (95% CI, 0.76 to 1.23), p < 0.00001], stride/step length [0.32 (95% CI, 0.03 to 0.61), p = 0.03], gait velocity [0.49 (95% CI, 0.20 to 0.78), p = 0.0009], Unified Parkinson’s Disease Rating Scale Part-III [-0.40 (95% CI, −0.55 to −0.24), p < 0.00001], and 6-minute walking test [0.35 (95% CI, 0.13 to 0.56), p = 0.002] in people with PD, but not in step cadence [−0.08 (95% CI, −0.43 to 0.27), p = 0.65] and Parkinson’s Disease Questionnaire-39 [−0.113 (95% CI, −0.39 to 0.13), p = 0.32]. Aerobic exercise had beneficial effects in improving balance, gait (velocity and stride/step length), and motor function in PD patients. However, aerobic exercise had no significant associations with the step cadence and quality of life in PD patients.
Characterization of B-cell and T-cell responses to a tetravalent dengue purified inactivated vaccine in healthy adults
Authors: Friberg Heather , Gargulak Morgan , Kong Amanda , Lin Leyi , Martinez Luis J. , Schmidt Alexander C. , Paris Robert M. , Jarman Richard G. , Diaz Clemente , Thomas Stephen J. , Moris Philippe , Currier Jeffrey R.
Published: October 2022

The increasing global impact of dengue underscores the need for a dengue virus (DENV) vaccine. We assessed B-cell and T-cell responses following vaccination with four formulations of a tetravalent dengue purified inactivated vaccine (DPIV) in dengue-primed and dengue-naive adults from two studies (NCT01666652, NCT01702857). Frequencies of DPIV-induced memory B cells specific to each DENV serotype remained high up to 12 months post-vaccination, and were higher in the dengue-primed than dengue-naive adults. A subsequent DPIV booster dose induced strong anamnestic B-cell responses. Multifunctional CD4+ T cells (predominantly expressing IL-2) were induced by DPIV, with higher frequencies in dengue-primed adults. DPIV-induced CD4+ T cells also demonstrated in vitro proliferative capacity and antigen-specific production of GM-CSF, IFN-γ, and IL-13. CD8+ T-cell responses were undetectable in dengue-naive adults and low in dengue-primed individuals. B- and T-cell responses persisted up to 12 months post-vaccination in both dengue-primed and dengue-naive adults.
TAFA4-IL-10 axis potentiate immunotherapy for airway allergy by induction of specific regulatory T cells
Authors: Qiu Shuyao , Luo Xiangqian , Mo Lihua , Zhang Shuang , Liao Yun , Guan Li , Yang Liteng , Huang Qinmiao , Liu Dabo , Yang Pingchang
Published: October 2022

Allergen-specific immunotherapy (AIT) is the main treatment for allergic diseases. The therapeutic efficacy of AIT has to be improved. Neuropeptides, such as TAFA4, have immune-regulating features. The objective of this study is to promote the efficacy of AIT in experimental allergic rhinitis (AR) by the concurrent use of TAFA chemokine as a family member 4 (TAFA4). In this study, an AR mouse model was developed using ovalbumin (OVA) as the specific antigen. The AR response was assessed in mice after treatment with AIT or/and TAFA4. We found that exposure to TAFA4 activated dendritic cells (DCs) in the airway tissues. Activation of DC by TAFA4 resulted in the expression of IL-10. TAFA4 also promoted the activities of c-Maf inducing protein. The FPR1-MyD88-AKT signal pathway was associated with the TAFA4-induced Il10 expression in the DCs. Co-administration of AIT/TAFA4 attenuated the AR response in mice by inducing antigen-specific Tr1 cells. In conclusion, TAFA4 induces the expression of IL-10 in DCs. Acting as an adjuvant, TAFA4 significantly improves AIT’s therapeutic efficacy against AR by inducing antigen-specific Tr1 cells.
Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing
Authors: Adler Benjamin A. , Hessler Tomas , Cress Brady F. , Lahiri Arushi , Mutalik Vivek K. , Barrangou Rodolphe , Banfield Jillian , Doudna Jennifer A.
Published: October 2022

CRISPR-Cas13 proteins are RNA-guided RNA nucleases that defend against incoming RNA and DNA phages by binding to complementary target phage transcripts followed by general, non-specific RNA degradation. Here we analysed the defensive capabilities of LbuCas13a from Leptotrichia buccalis and found it to have robust antiviral activity unaffected by target phage gene essentiality, gene expression timing or target sequence location. Furthermore, we find LbuCas13a antiviral activity to be broadly effective against a wide range of phages by challenging LbuCas13a against nine E. coli phages from diverse phylogenetic groups. Leveraging the versatility and potency enabled by LbuCas13a targeting, we applied LbuCas13a towards broad-spectrum phage editing. Using a two-step phage-editing and enrichment method, we achieved seven markerless genome edits in three diverse phages with 100% efficiency, including edits as large as multi-gene deletions and as small as replacing a single codon. Cas13a can be applied as a generalizable tool for editing the most abundant and diverse biological entities on Earth.
Probing coenzyme A homeostasis with semisynthetic biosensors
Authors: Xue Lin , Schnacke Paul , Frei Michelle S. , Koch Birgit , Hiblot Julien , Wombacher Richard , Fabritz Sebastian , Johnsson Kai
Published: October 2022

Coenzyme A (CoA) is one of the central cofactors of metabolism, yet a method for measuring its concentration in living cells is missing. Here we introduce the first biosensor for measuring CoA levels in different organelles of mammalian cells. The semisynthetic biosensor is generated through the specific labeling of an engineered GFP–HaloTag fusion protein with a fluorescent ligand. Its readout is based on CoA-dependent changes in Förster resonance energy transfer efficiency between GFP and the fluorescent ligand. Using this biosensor, we probe the role of numerous proteins involved in CoA biosynthesis and transport in mammalian cells. On the basis of these studies, we propose a cellular map of CoA biosynthesis that suggests how pools of cytosolic and mitochondrial CoA are maintained.
LifeSnaps, a 4-month multi-modal dataset capturing unobtrusive snapshots of our lives in the wild
Authors: Yfantidou Sofia , Karagianni Christina , Efstathiou Stefanos , Vakali Athena , Palotti Joao , Giakatos Dimitrios Panteleimon , Marchioro Thomas , Kazlouski Andrei , Ferrari Elena , Girdzijauskas Šarūnas
Published: October 2022

Ubiquitous self-tracking technologies have penetrated various aspects of our lives, from physical and mental health monitoring to fitness and entertainment. Yet, limited data exist on the association between in the wild large-scale physical activity patterns, sleep, stress, and overall health, and behavioral and psychological patterns due to challenges in collecting and releasing such datasets, including waning user engagement or privacy considerations. In this paper, we present the LifeSnaps dataset, a multi-modal, longitudinal, and geographically-distributed dataset containing a plethora of anthropological data, collected unobtrusively for the total course of more than 4 months by n = 71 participants. LifeSnaps contains more than 35 different data types from second to daily granularity, totaling more than 71 M rows of data. The participants contributed their data through validated surveys, ecological momentary assessments, and a Fitbit Sense smartwatch and consented to make these data available to empower future research. We envision that releasing this large-scale dataset of multi-modal real-world data will open novel research opportunities and potential applications in multiple disciplines.
On the analyses of carbon atom diffused into grey cast iron during carburisation process
Authors: Salawu Enesi Y. , Adediran Adeolu A. , Ajayi Oluseyi O. , Inegbenebor Anthony O. , Dirisu Joseph O.
Published: October 2022

The study employed Fick’s second law of diffusion to discover some unknown aspect of carbon diffusion in grey cast iron during carburisation process. Emphasis on the experiments and theoretical modelling were established for better accomplishments. Pulverised palm kernel and eggshell additives of 70 (wt.%) and 30 (wt.%) according to the Voige law of mixture was considered as a continuous medium without considering the atomic nature of the mixture. Furthermore, a kinetic approach was described where a physical model of the substrate immersed in the carbon mixture was established while diffusion equations were modelled to establish the mechanism of carbon diffusion during carburisation. Initial composition and concentration of diffused atom remained constant which are 2.68 and 6.67% carbon. While the carburizing time used varied from 60 min, 90 min, 120 min, 150 min, 180 min and 210 min respectively at constant carburising temperature of 900° The results revealed varying composition gradient of carbon atom ranging from 5.4%, 5.42%, 5.44%, 5.46%, 5.51%, and 5.65 compared to the initial carbon content of 2.68%. The concentration of carbon atom on the substrate surface at varying time implies that the process was non-steady state diffusion which verified Fick’s second law of diffusion. Hence, the composition achieved is a function of boundary conditions such as time position and temperature. This novel study will enhance the understanding of heat treat treatment of metals such that their applications in the industry will be numerous.
Hypothermia and heart rate variability in a healthy newborn piglet model
Authors: Pedersen Mette Vestergård , Andelius Ted Carl Kejlberg , Andersen Hannah Brogård , Kyng Kasper Jacobsen , Henriksen Tine Brink
Published: October 2022

Decreased heart rate variability (HRV) may be a biomarker of brain injury severity in neonatal hypoxic-ischemic encephalopathy for which therapeutic hypothermia is standard treatment. While therapeutic hypothermia may influence the degree of brain injury; hypothermia may also affect HRV per se and obscure a potential association between HRV and hypoxic-ischemic encephalopathy. Previous results are conflicting. This study aimed to investigate the effect of hypothermia on HRV in healthy, anaesthetised, newborn piglets. Six healthy newborn piglets were anaesthetised. Three piglets were first kept normothermic (38.5–39.0 °C) for 3 h, then exposed to hypothermia (33.5–34.5 °C) for 3 h. Three piglets were first exposed to hypothermia for 3 h, then rewarmed to normothermia for 3 h. Temperature and ECG were recorded continuously. HRV was calculated from the ECG in 5 min epochs and included time domain and frequency domain variables. The HRV variables were compared between hypothermia and normothermia. All assessed HRV variables were higher during hypothermia compared to normothermia. Heart rate was lower during hypothermia compared to normothermia and all HRV variables correlated with heart rate. Hypothermia was associated with an increase in HRV; this could be mediated by bradycardia during hypothermia.
Differential expression and regulation of HSP70 gene during growth phase in ruminants in response to heat stress
Authors: Kaushik Rakesh , Goel Anjana , Rout P. K.
Published: October 2022

Heat shock proteins regulate the physiological mechanism of heat stress adaptation at cellular level. The present investigation was carried out to analyse the HSP70 gene regulation in various growth stage in ruminants in peripheral blood mononuclear cells (PBMCs). The relationship between HSP gene expression and thermotolerance in age-specific manner in ruminants has not been analysed. Therefore m-RNA HSP70 expression level was examined in different age groups of Jamunpari goat during hot climatic conditions. The experiment was carried out in 32 animals of Jamunapari goat belonging to the age groups of 3-months, 9-months, 12-months, and adults (2–3 year). Total RNA was isolated from peripheral blood mononuclear cells. The physiological response such as rectal temperature (RT), respiration rate (RR) and heart rate (HR) was used as indicator to heat stress. Temperature Humidity Index (THI) was used as an indicator of severity of environmental stress. The THI range varied from 82.00–92.08 during experimental period. The m-RNA HSP70 expression level at 9-month age of animals was up-regulated and significantly higher than other age groups. It was observed that the level of HSP70 transcripts in PBMCs was highest at 9-month age group, and age-related decline in HSP70 expression was observed in adult age. Based on the physiological response, the contrasting heat-stress phenotypes were recognised as heat stress susceptible (HSS) and heat stress tolerant (HST) individuals and the expression of m-RNA HSP70 was analysed at different ages in response to chronic heat stress. The differential mRNA expression of HSS individuals at 3 and 9-month of age showed the highest fold expression than HST. Age and phenotype had significant effect (p < 0.01) on the crossing point (CP) value. The m-RNA HSP70 gene expression in different age groups was correlated with heat stress tolerance and this could be used as biomarker for breeders to analyse the HSP responsein -vivoin ruminants.
Comprehensive single-shot biophysical cytometry using simultaneous quantitative phase imaging and Brillouin spectroscopy
Authors: Steelman Zachary A. , Coker Zachary N. , Sedelnikova Anna , Keppler Mark A. , Kiester Allen S. , Troyanova-Wood Maria A. , Ibey Bennett L. , Bixler Joel N.
Published: October 2022

Single-cell analysis, or cytometry, is a ubiquitous tool in the biomedical sciences. Whereas most cytometers use fluorescent probes to ascertain the presence or absence of targeted molecules, biophysical parameters such as the cell density, refractive index, and viscosity are difficult to obtain. In this work, we combine two complementary techniques—quantitative phase imaging and Brillouin spectroscopy—into a label-free image cytometry platform capable of measuring more than a dozen biophysical properties of individual cells simultaneously. Using a geometric simplification linked to freshly plated cells, we can acquire the cellular diameter, volume, refractive index, mass density, non-aqueous mass, fluid volume, dry volume, the fractional water content of cells, both by mass and by volume, the Brillouin shift, Brillouin linewidth, longitudinal modulus, longitudinal viscosity, the loss modulus, and the loss tangent, all from a single acquisition, and with no assumptions of underlying parameters. Our methods are validated across three cell populations, including a control population of CHO-K1 cells, cells exposed to tubulin-disrupting nocodazole, and cells under hypoosmotic shock. Our system will unlock new avenues of research in biophysics, cell biology, and medicine.