bluuebunny/crossref_metadata_2025 huggingface dataset:
Differentiation of Human-Induced Pluripotent Stem Cell-Derived Endocrine Progenitors to Islet-like Cells Using a Dialysis Suspension Culture System
Authors: Choi Hyunjin , Shinohara Marie , Ibuki Masato , Nishikawa Masaki , Sakai Yasuyuki
Published: August 2021

The production of functional islet-like cells from human-induced pluripotent stem cells (hiPSCs) is a promising strategy for the therapeutic use and disease modeling for type 1 diabetes. However, the production cost of islet-like cells is extremely high due to the use of expensive growth factors for differentiation. In a conventional culture method, growth factors and beneficial autocrine factors remaining in the culture medium are removed along with toxic metabolites during the medium change, and it limits the efficient utilization of those factors. In this study, we demonstrated that the dialysis suspension culture system is possible to reduce the usage of growth factors to one-third in the differentiation of hiPSC-derived endocrine progenitor cells to islet-like cells by reducing the medium change frequency with the refinement of the culture medium. Furthermore, the expression levels of hormone-secretion-related genes and the efficiency of differentiation were improved with the dialysis suspension culture system, possibly due to the retaining of autocrine factors. In addition, we confirmed several improvements required for the further study of the dialysis culture system. These findings showed the promising possibility of the dialysis suspension culture system for the low-cost production of islet-like cells.
Improving the Assessment of Neonatal Abstinence Syndrome (NAS)
Authors: Chin Foo Claire A. , Dansereau Lynne M. , Hawes Katheleen , Oliveira Erica L. , Lester Barry M.
Published: August 2021

Neonatal Abstinence Syndrome (NAS) is a public health problem of epidemic proportions. The Finnegan Neonatal Abstinence Scoring System (FNASS) is the tool most widely used to evaluate NAS. However, it is limited by its lack of interrater reliability and standardized approach. Surveys to evaluate the FNASS were distributed to nurses at the Women and Infants Hospital in Providence, RI, USA. Infants (n = 78) treated for NAS and born to methadone-maintained mothers were examined to compare items administered from the FNASS and the NICU Network Neurobehavioral Scale (NNNS). All nurses reported that the FNASS was somewhat to very subjective. More than half reported that it was somewhat to not accurate and a new scoring method is needed to accurately diagnose NAS. Correlations between FNASS items and NNNS items showed 9 of 32 (28.1%) correlations were strong (rs > 0.5), 5 of 32 (15.6%) were moderate (0.3 < rs < 0.5), and 10 of 32 (31.3%) were weak (0.1 < rs < 0.3). Principal component factor analysis (PCA) of the NNNS explained more variance (35.1%) than PCA of NNNS and FNASS items combined (33.1%). The nursing survey supported the need for developing a more objective exam to assess NAS. NNNS exam items may be used to improve the evaluation of NAS.
Descending Mediastinitis in a Child: Diagnostic Challenges and a Different Treatment Approach
Authors: Lira Joana , Santos Joana , Capela Mariana , Rodrigues Joana , Cunha Otília , Carvalho Isabel
Published: August 2021

In children, spontaneous mediastinitis is a rare, severe and commonly misdiagnosed disease. Although standard of care treatment frequently involves surgery, we report a case of mediastinitis in a five-year-old child, successfully treated with 4 weeks of intravenous antibiotics. Ultrasound imaging was used to monitor patient response to conservative treatment while reducing radiation exposure.
Synthesis, Microstructure and Properties of Magnetron Sputtered Lead Zirconate Titanate (PZT) Thin Film Coatings
Authors: Ma Youcao , Song Jian , Wang Xubo , Liu Yue , Zhou Jia
Published: August 2021

Compared to aluminum nitride (AlN) with simple stoichiometry, lead zirconate titanate thin films (PZT) are the other promising candidate in advanced micro-electro-mechanical system (MEMS) devices due to their excellent piezoelectric and dielectric properties. The fabrication of PZT thin films with a large area is challenging but in urgent demand. Therefore, it is necessary to establish the relationships between synthesis parameters and specific properties. Compared to sol-gel and pulsed laser deposition techniques, this review highlights a magnetron sputtering technique owing to its high feasibility and controllability. In this review, we survey the microstructural characteristics of PZT thin films, as well as synthesis parameters (such as substrate, deposition temperature, gas atmosphere, and annealing temperature, etc.) and functional proper-ties (such as dielectric, piezoelectric, and ferroelectric, etc). The dependence of these influential factors is particularly emphasized in this review, which could provide experimental guidance for researchers to acquire PZT thin films with expected properties by a magnetron sputtering technique.
The Electrochemical Reduction Mechanism of ZnFe2O4 in NaCl-CaCl2 Melts
Authors: Liu Chang , Liang Jinglong , Li Hui , Yan Hongyan , Zheng Sijia , Cao Weigang , Wang Le
Published: August 2021

The electrochemical reduction process of ZnFe2O4 in NaCl-CaCl2 melts was studied. Thermodynamic analysis shows that the reduction process of ZnFe2O4 is carried out in multiple steps, and it is difficult to reduce Fe3+ to Fe in one step. Electrochemical tests revealed that the reduction process of ZnFe2O4 includes three steps: First, Fe3+ is reduced to Fe in two steps, then Zn2+ is reduced to Zn in one step. The reduction of Fe3+ on the Mo electrode is a reversible process controlled by diffusion, while the reduction of Zn2+ is an irreversible process controlled by diffusion. The influence of electrolysis voltage and temperature on the process of electric deoxidation has also been studied. It is indicated that properly increasing the temperature is conducive to the diffusion of oxygen ions, thereby increasing the deoxidation rate. With the gradual increase of voltage, the reduction process of ZnFe2O4 is ZnFe2O4 → FeO + ZnO → Fe + ZnO → Fe + Zn.
The Diversity of Culture-Dependent Gram-Negative Rhizobacteria Associated with Manihot esculenta Crantz Plants Subjected to Water-Deficit Stress
Authors: Zapata Tatiana , Galindo Diana Marcela , Corrales-Ducuara Alba Rocío , Ocampo-Ibáñez Iván Darío
Published: August 2021

There is a lack of studies on the root-associated bacterial microbiome of cassava plants. The identification and characterization of rhizobacteria can contribute to understanding the adaptation of the agriculturally important crop plants to abiotic stress. Rhizobacteria play a significant role in plants, as they can alleviate the drought stress by various mechanisms that enhance the plant growth under these stressor conditions. In this study, Gram-negative bacterial strains from the plant rhizosphere of cassava Manihot esculenta Crantz CIAT MCOL1734 variety subjected to water deprivation were isolated, characterized according to their morphological properties, and then identified by VITEK® 2. An increase in the diversity, abundance, and species richness of Gram-negative rhizobacterial community was found in cassava plants subjected to water-deficit stress. In total, 58 rhizobacterial strains were isolated from cassava plants. The identification process found that the bacteria belonged to 12 genera: Achromobacter, Acinetobacter, Aeromonas, Buttiauxella, Cronobacter, Klebsiella, Ochrobactrum, Pluralibacter, Pseudomonas, Rhizobium, Serratia, and Sphingomonas. Interestingly, Pseudomonas luteola and Ocrhobactrum anthropi were rhizobacteria isolated exclusively from plants submitted to drought conditions. The cassava roots constitute a great reservoir of Gram-negative bacteria with a remarkable potential for biotechnological application to improve the drought tolerance of plant crops under water-deficit conditions.
Mask-Related Motion Artifact on 99mTc-MIBI SPECT: Unexpected Pitfalls of SARS-CoV-2 Countermeasures
Authors: Cichocki Paweł , Adamczewski Zbigniew , Kuśmierek Jacek , Płachcińska Anna
Published: August 2021

A 61-year-old man was referred for myocardial perfusion scintigraphy (MPS) by an occupational physician to exclude coronary artery disease (CAD). The patient had a complete left bundle branch block (LBBB) that rendered the routine exercise stress test non-diagnostic, but otherwise had no history of heart diseases, good stress tolerance with no symptoms of angina, and no abnormalities in transthoracic echocardiogram, apart from contraction patterns typical for LBBB. Initial MPS, performed using technetium-labeled Sestamibi on a Discovery NM 530c camera equipped with solid-state semiconductor detectors, revealed a significant stress-induced ischemia that did not match the good overall condition of the patient. A motion detection procedure revealed significant heart motion in Z-axis during the stress study. Upon inquiry, the patient reported breathing difficulties caused by the mandatory mask, which slipped into an uncomfortable position during the study. Repeated acquisition, without motion artifacts, revealed no features of ischemia.
Comprehensive Assessment of Medial Knee Joint Instability by Valgus Stress MRI
Authors: Ciba Malin , Winkelmeyer Eva-Maria , Schock Justus , Schad Philipp , Kotowski Niklas , Nolte Teresa , Wollschläger Lena Marie , Knobe Matthias , Prescher Andreas , Kuhl Christiane , Truhn Daniel , Nebelung Sven
Published: August 2021

Standard clinical MRI techniques provide morphologic insights into knee joint pathologies, yet do not allow evaluation of ligament functionality or joint instability. We aimed to study valgus stress MRI, combined with sophisticated image post-processing, in a graded model of medial knee joint injury. To this end, eleven human cadaveric knee joint specimens were subjected to sequential injuries to the superficial medial collateral ligament (sMCL) and the anterior cruciate ligament (ACL). Specimens were imaged in 30° of flexion in the unloaded and loaded configurations (15 kp) and in the intact, partially sMCL-deficient, completely sMCL-deficient, and sMCL- and ACL-deficient conditions using morphologic sequences and a dedicated pressure-controlled loading device. Based on manual segmentations, sophisticated 3D joint models were generated to compute subchondral cortical distances for each condition and configuration. Statistical analysis included appropriate parametric tests. The medial compartment opened gradually as a function of loading and injury, especially anteriorly. Corresponding manual reference measurements by two readers confirmed these findings. Once validated in clinical trials, valgus stress MRI may comprehensively quantify medial compartment opening as a functional imaging surrogate of medial knee joint instability and qualify as an adjunct diagnostic tool in the differential diagnosis, therapeutic decision-making, and monitoring of treatment outcomes.
Robust Nonlinear Tracking Control for Unmanned Aircraft in the Presence of Wake Vortex
Authors: Kazarin Petr , Golubev Vladimir , MacKunis William , Moreno Claudia
Published: August 2021

The flight trajectory of unmanned aerial vehicles (UAVs) can be significantly affected by external disturbances such as turbulence, upstream wake vortices, or wind gusts. These effects present challenges for UAV flight safety. Hence, addressing these challenges is of critical importance for the integration of unmanned aerial systems (UAS) into the National Airspace System (NAS), especially in terminal zones. This work presents a robust nonlinear control method that has been designed to achieve roll/yaw regulation in the presence of unmodeled external disturbances and system nonlinearities. The data from NASA-conducted airport experimental measurements as well as high-fidelity Large Eddy Simulations of the wake vortex are used in the study. Side-by-side simulation comparisons between the robust nonlinear control law and both linear H∞ and PID control laws are provided for completeness. These simulations are focused on applications involving small UAV affected by the wake vortex disturbance in the vicinity of the ground (which models the take-off or landing phase) as well as in the out-of-ground zone. The results demonstrate the capability of the proposed nonlinear controller to asymptotically reject wake vortex disturbance in the presence of the nonlinearities in the system (i.e., parametric variations, unmodeled, time-varying disturbances). Further, the nonlinear controller is designed with a computationally efficient structure without the need for the complex calculations or function approximators in the control loop. Such a structure is motivated by UAV applications where onboard computational resources are limited.
Study on the Effect of Gaze Position and Image Brightness on Peripheral Dimming Technique
Authors: Kim Jeong-Sik , Jeong Won-Been , An Byeong Hun , Lee Seung-Woo
Published: August 2021

Here, we study a low-power technique for displays based on gaze tracking, called peripheral dimming. In this work, the threshold levels of the lightness reduction ratio (LRR), where people notice differences in brightness, depending on gaze positions and image brightness, are investigated. A psychophysical experiment with five gaze positions and three image brightness conditions is performed, and the estimated threshold levels are obtained. To investigate the significance of the differences between the threshold levels, the overlap method and the Bayesian estimation (BEST) analysis are performed. The analysis results show that the difference of the threshold levels depending on the conditions is insignificant. Thus, the proposed technique can operate with a constant LRR level, regardless of the gaze position or image brightness, while maintaining the perceptual image quality. In addition, the proposed technique reduces the power consumption of virtual reality (VR) displays by 12–14% on average. We believe that the peripheral dimming technique would contribute to reducing the power of the self-luminous displays used for VR headsets with an integrated eye tracker.
Feature Matching Synchronized Reasoning from Energy-Based Memory Network for Intelligent Data Management in Cloud Computing Data Center
Authors: Shim JeongYon
Published: August 2021

A cloud data center for software-as-a-service (SaaS) was built for the purpose of stably managing these server computers in one place in order to provide an uninterrupted service, not only for a stable power supply and security but also for the efficient data management. To manage such a data center efficiently, it is important to build a cloud database with structured storage above all else. In recent decades, many studies have focused on designing cloud data centers and most of the research has focused on communication traffic, routing, topological issues and communication technology. However, in order to build an efficient cloud database that can support user demand, the most sophisticated intelligent system, based on AI technology and considering user convenience, should be designed. From this viewpoint, adopting human brain functions, Energy-Based Memory Network was designed for a knowledge-based frame of an intelligent system. And its event-related synchronized data extraction mechanism was proposed. In particular, a Thinking Thread extraction phase was implemented for the reasoning process using qualia matching and a deep extraction method in a cloud database. The purpose of this approach is to design and implement an intelligent cloud database that has an efficient structure and mechanism for supporting user demand and providing accurate, prompt services. In experiments, the working phase of the functions was simulated with data and analyzed. As a result, it was confirmed that the proposed system works well and intelligently for the design purpose.
The Linkage of Soil CO2 Emissions in a Moso Bamboo (Phyllostachysedulis (Carriere) J. Houzeau) Plantation with Aboveground and Belowground Stoichiometry
Authors: Tian Xiaokun , Ge Xiaogai , Zhou Benzhi , Li Maihe
Published: August 2021

Understanding the effects of soil stoichiometry and nutrient resorption on soil CO2 emissions is critical for predicting forest ecosystem nutritional demands and limitations tooptimal forest growth. In this study, we examined the effects of above- and belowground stoichiometry on soil CO2 emissions and their mediating effect on soil respiration in subtropical moso bamboo (Phyllostachys edulis) plantations. Our results showed that the soil respiration rate did not differ significantly among four bamboo stands. Nitrogen (N) and phosphorous (P) concentrations were higher in bamboo leaves than litter, whereas the C:N and C:P ratios showed the opposite trend. Significant positive correlations of soil cumulative CO2 emission with litter C:P (p = 0.012) and N:P (p = 0.041) ratios indicated that litter stoichiometry was a better predictor of soil respiration than aboveground stoichiometry. Cumulative soil CO2 emissions were significantly negatively correlated with soil microbe C:N (p = 0.021) and C:N (p = 0.036) ratios, and with soil respiratory quotients (p < 0.001). These results suggest that litter and soil stoichiometry are reliable indicators of the soil respiration rate. This study provides important information about the effects of ecosystem stoichiometry and soil microbial biomass on soil CO2 emissions and highlights them editing role of soil nutritional demands and limitations in the association between soil respiration rates and aboveground plant tissues.
Radial Growth Response of Scots Pine (Pinus sylvestris L.) after Increasing the Availability of Water Resources
Authors: Czyżyk Krzysztof
Published: August 2021

In the years 1998–2022, about eight thousand hydrotechnical facilities were built or planned for construction at the State Forests National Forest Holding in Poland, which could slow down the outflow of water from small forest catchments and store over 50 hm³ of water. Due to the innovative and unprecedented nature of investments retaining water resources on forest land, having a significant impact on the adaptation and mitigation of the negative effects of climate change, the literature on the subject lacks in studies describing their impact on the adjacent forest environment. The aim of the presented research is to determine the evolution of the tree-ring width of Scots pine (Pinus sylvestris L.) before and after the reconstruction of two water reservoirs and the construction of gates in drainage ditches as part of small retention projects in lowland areas. The research was carried out on the basis of core drillings collected in four forest districts and climate data provided by the Polish Institute of Meteorology and Water Management—National Research Institute. In the first stage of the work, no statistically significant influence of climatic conditions on the shaping of growths was demonstrated. In the next step, the variability of increments and their width in individual years before and after the investment was examined. Then, the distance of individual trees from reservoirs and drainage ditches was analyzed, as well as its importance in forming increments. The calculated statistical significance of differences in increments, average, minimum and maximum values, as well as standard deviation indicated the possible influence of retention reservoirs and valves on shaping the increments. The study did not confirm the importance of the distance of trees from the newly available water resources in the growth of the width of the increments.
Securing SDN-Based IoT Group Communication
Authors: Alzahrani Bander , Fotiou Nikos
Published: August 2021

IoT group communication allows users to control multiple IoT devices simultaneously. A convenient method for implementing this communication paradigm is by leveraging software-defined networking (SDN) and allowing IoT endpoints to “advertise” the resources that can be accessed through group communication. In this paper, we propose a solution for securing this process by preventing IoT endpoints from advertising “fake” resources. We consider group communication using the constrained application protocol (CoAP), and we leverage Web of Things (WoT) Thing Description (TD) to enable resources’ advertisement. In order to achieve our goal, we are using linked-data proofs. Additionally, we evaluate the application of zero-knowledge proofs (ZKPs) for hiding certain properties of a WoT-TD file.
Authentication and Chemometric Discrimination of Six Greek PDO Table Olive Varieties through Morphological Characteristics of Their Stones
Authors: Agriopoulou Sofia , Tarapoulouzi Maria , Bedine Boat Marie Ampères , Rébufa Catherine , Dupuy Nathalie , Theocharis Charis R. , Varzakas Theodoros , Roussos Sevastianos , Artaud Jacques
Published: August 2021

Table olives, the number one consumed fermented food in Europe, are widely consumed as they contain many valuable ingredients for health. It is also a food which may be the subject of adulteration, as many different olive varieties with different geographical origin, exist all over the word. In the present study, the image analysis of stones of six main Greek protected designation of origin (PDO) table olive varieties was performed for the control of their authentication and discrimination, with cv. Prasines Chalkidikis, cv. Kalamata Olive, cv. Konservolia Stylidas, cv. Konservolia Amfissis, cv. Throuba Thassos and cv. Throuba Chios being the studied olive varieties. Orthogonal partial least square discriminant analysis (OPLS-DA) was used for discrimination and classification of the six Greek table olive varieties. With a 98.33% of varietal discrimination, the OPLS-DA model proved to be an efficient tool to authentify table olive varieties from their morphological characteristics.
Spinal and Pelvic Alignment of Sitting Posture Associated with Smartphone Use in Adolescents with Low Back Pain
Authors: In Tae-sung , Jung Jin-hwa , Jung Kyoung-sim , Cho Hwi-young
Published: August 2021

This study aimed to assess the association between smartphone use in the sitting posture and changes in thoracolumbar kyphosis, lumbar lordosis, and pelvic asymmetry in adolescents with low back pain (LBP). Twenty-five adolescents with LBP and 25 healthy adolescents participated in this study. They were instructed to sit on a height-adjustable chair with their hips and knees bent at 90° for 30 min in their usual sitting postures. Thoracolumbar kyphosis, lumbar lordosis, and pelvic asymmetry were measured using a three-dimensional motion capture system. Thoracolumbar kyphosis and lumbar lordosis increased after 30 min of sitting compared to the baseline. In both groups, thoracic kyphosis and lumbar lordosis angle increased with increasing sitting time. Compared to healthy adolescents, adolescents with LBP presented greater thoracolumbar kyphosis and lumbar lordosis after prolonged sitting. Pelvic asymmetry showed no significant difference between the sitting time and groups. Using a smartphone during prolonged sitting may lead to a slumped posture; these associations were more pronounced in adolescents with LBP.
Pickleball for Inactive Mid-Life and Older Adults in Rural Utah: A Feasibility Study
Authors: Wray Paige , Ward Callahan K. , Nelson Cindy , Sulzer Sandra H. , Dakin Christopher J. , Thompson Brennan J. , Vierimaa Matthew , Das Gupta Debasree , Bolton David A. E.
Published: August 2021

Many diseases, disabilities, and mental health conditions associated with aging can be delayed or prevented through regular exercise. Several barriers to exercise, many of which are exacerbated in rural communities, prevent mid-life and older adults from accessing its benefits. However, recently, a racquet sport named pickleball has become popular among older adults, and it appears to overcome some of these barriers. We conducted a feasibility study to evaluate the impact of a six-week pickleball intervention on measures of muscle function, cognitive function, perceived pain, and cardio-metabolic risk, as well as several psychosocial factors contributing to adherence in sedentary rural participants. Participants improved their vertical jump, cognitive performance, and reported a decrease in self-reported pain, suggesting improved physical and cognitive health across the sample. Participants also reported high levels of satisfaction and demonstrated good adherence over the duration of the study. Perhaps of greatest value was the overwhelmingly positive response from participants to the intervention and follow-up interviews reporting a desire to continue pickleball play beyond the study period. Overall, pickleball appears to be a promising intervention to, (1) elicit functional- and cognitive-related improvements, and (2) motivate mid-life and older adults to adhere to exercise sufficiently long to benefit their health.
Cultural Considerations for the Adaptation of a Diabetes Self-Management Education Program in Cotonou, Benin: Lessons Learned from a Qualitative Study
Authors: Alaofè Halimatou , Yeo Sarah , Okechukwu Abidemi , Magrath Priscilla , Amoussa Hounkpatin Waliou , Ehiri John , Rosales Cecilia
Published: August 2021

Background: Type 2 diabetes (T2D) poses a disproportionate burden on Benin, West Africa. However, no diabetes intervention has yet been developed for Benin’s contexts. This study aimed to explore specific cultural beliefs, attitudes, behaviors, and environmental factors to help adapt a diabetes self-management program to patients with T2D from Cotonou, in southern Benin. Methods: Qualitative data were collected through focus group discussions (FDGs) involving 32 patients with T2D, 16 academic partners, and 12 community partners. The FDGs were audio-recorded, transcribed verbatim from French to English, and then analyzed thematically with MAXQDA 2020. Results: Healthy food was challenging to obtain due to costs, seasonality, and distance from markets. Other issues discussed were fruits and vegetables as commodities for the poor, perceptions and stigmas surrounding the disease, and the financial burden of medical equipment and treatment. Information about local food selections and recipes as well as social support, particularly for physical activity, were identified, among other needs. When adapting the curriculum, gender dynamics and spirituality were suggested. Conclusions: The study demonstrates the need for culturally sensitive interventions and a motivation-based approach to health (spiritual and emotional support). It also lays the groundwork for addressing T2D contextually in Benin and similar sub-Saharan African countries.
Assessment of the Emission of Pollutants from Public Transport Based on the Example of Diesel Buses and Trolleybuses in Gdynia and Sopot
Authors: Połom Marcin , Wiśniewski Paweł
Published: August 2021

The present study attempts to examine the research gap in terms of comparing the environmental impact of trolleybuses and diesel buses in the conditions of a country with an unfavourable energy mix. The analysed example concerns the trolleybus transport system in Gdynia, in northern Poland, which also partially serves the neighbouring city of Sopot. In the last few years, two bus lines have been electrified with trolleybuses in the In-Motion-Charging technology, which enables operation on sections without an overhead network. Using the actual operational data, a comparative analysis of the emissivity of diesel buses and trolleybuses used on the same lines in an identical operating regime was conducted. Moreover, an attempt was made to estimate the damage costs of the emission of air pollutants for the above-mentioned means of transport. Research has shown that trolleybuses significantly help to reduce emissions of nitrogen oxides, non-methane volatile organic compounds and particulate matter, while increasing sulphur dioxide emissions on the served lines. They also generate lower specific emissions of carbon dioxide compared to diesel buses. However, taking into account the differences in the number of seats in these vehicles, the length of routes resulting from a need to provide access to the necessary infrastructure and the total amount of kilometres covered on a given route, they may cause higher emissions per year and per the product life cycle than diesel buses. This is related to the unfavourable structure of energy production in Poland, which is dominated by coal sources. The research results clearly show that the use of trolleybuses in public transport contributes to a reduction of the damage costs of the emission of pollutants that amount to approximately EUR (€) 30,000–60,000 per year for the analysed lines.
The Effect of Static and Dynamic Stretching during Warm-Up on Running Economy and Perception of Effort in Recreational Endurance Runners
Authors: Faelli Emanuela , Panascì Marco , Ferrando Vittoria , Bisio Ambra , Filipas Luca , Ruggeri Piero , Bove Marco
Published: August 2021

This randomized crossover counterbalanced study investigated, in recreational runners, the acute effects of pre-exercise stretching on physiological and metabolic responses, endurance performance, and perception of effort. Eight male endurance runners (age 36 ± 11 years) performed three running-until-exhaustion tests, preceded by three warm-ups, including the following different stretching protocols: static (SS), dynamic (DS), and no-stretching (NS). During the SS and DS sessions, the warm-up consisted of 10 min of running plus 5 min of SS or DS, respectively, while during the NS session, the warm-up consisted of 15 min of running. Physiological and metabolic responses, and endurance running performance parameters, were evaluated. The perception of effort was derived from the rating of perceived exertion (RPE). Running economy significantly improved after SS (p < 0.05) and DS (p < 0.01), and RPE values were significantly lower in SS (p < 0.05) and DS (p < 0.01), compared to NS. No differences in physiological and metabolic responses among the sessions were found. This study showed that including SS and DS within the warm-up ameliorated running economy and decreased the perception of effort during a running-until-exhaustion test, highlighting the benefits of stretching on endurance performance. These results should encourage recreational runners to insert stretching during warm-up, to optimize the running energy costs, reducing the perception of effort and making the training sessions more enjoyable.
Association between Body Mass Index and Outcomes in Patients with Return of Spontaneous Circulation after Out-of-Hospital Cardiac Arrest: A Systematic Review and Meta-Analysis
Authors: Lee Heekyung , Shin Hyungoo , Oh Jaehoon , Lim Tae-Ho , Kang Bo-Seung , Kang Hyunggoo , Choi Hyuk-Joong , Kim Changsun , Park Jung-Hwan
Published: August 2021

Increased body mass index (BMI) is a risk factor for cardiovascular disease, stroke, and metabolic diseases. A high BMI may affect outcomes of post-cardiac arrest patients, but the association remains debatable. We aimed to determine the association between BMI and outcomes in patients with return of spontaneous circulation (ROSC) after out-of-hospital cardiac arrest (OHCA). A systematic literature search was conducted using MEDLINE, EMBASE, and the Cochrane Library. Studies that included patients who presented ROSC after OHCA, had a recorded BMI, and were assessed for neurological outcomes and in-hospital mortality were included. To assess the risk of bias of each included study, we employed the Risk of Bias Assessment Tool for Non-randomized Studies. We assessed 2427 patients from six studies. Neurological outcomes were significantly poorer in underweight patients (risk ratio (RR) = 1.21; 95% confidence interval (CI) = 1.07–1.37; p = 0.002; I2 = 51%) than in normal-weight patients. Additionally, in-hospital mortality rate was significantly higher in underweight patients (RR = 1.35; 95% CI = 1.14–1.60; p<0.001; I2 = 21%) and in obese patients (RR = 1.25; 95% CI = 1.12–1.39; p<0.001; I2 = 0%) than in normal-weight patients. Poor neurological outcome is associated with underweight, and low survival rate is associated with underweight and obesity in patients with ROSC after OHCA.
Social Connectivity, Sentiment and Participation on Twitter during COVID-19
Authors: Castro-Martinez Andrea , Méndez-Domínguez Paula , Sosa Valcarcel Aimiris , Castillo de Mesa Joaquín
Published: August 2021

In a transnational context defined by the irruption of COVID-19 and the social isolation it has generated around the world, social networking sites are essential channels for communicating and developing new forms of social coexistence based on connectivity and interaction. This study analyzes the feelings expressed on Twitter through the hashtags #YoMeQuedoEnCasa, #stayhome, #jeresteàlamaison, #restealamaison, #stoacasa, #restaacasa, #ficaemcasa, #euficoemcasa, #ichbleibezuHause and #Bleibzuhause, and the communicative and social processes articulated from network participation, during the lockdown in 2020. Through Gephi software, the aspects underlying the communicative interaction and the distribution of the network at a global level are studied, with the identification of leaderships, communities and connectivity nodes. As a result of this interaction, the emergence of social and organizational links derived from community participation and motivated by the common interest of preserving health and general wellbeing through collective action is detected. The study notes the presence of feelings of solidarity, a sense of community and social support among connected crowds who, despite being in geographically dispersed settings, share similar concerns about the virus effect.
Reducing Peak Energy Demand among Residents Who Are Not Billed for Their Electricity Consumption: Experimental Evaluation of Behaviour Change Interventions in a University Setting
Authors: Jorgensen Bradley S. , Fumei Sarah , Byrne Graeme
Published: August 2021

Behaviour change interventions aiming to reduce household energy consumption are regarded as an effective means to address disparities between demand and supply and reduce emissions. Less recognised is their success in shifting consumers’ energy consumption from peak demand periods to off-peak times of the day. This study reports two experiments that test the effect of feedback and reminder notifications on energy consumption in university halls-of-residence. A quasi-experiment and a randomised controlled experiment were conducted with residential students to evaluate behaviour change interventions aimed at reducing daily peak and critical peak demand, respectively. The results of Experiment One (n = 143) demonstrated significant reductions in the energy use of the treatment group relative to the control. On average, the treatment group’s energy use was 12.4 per cent lower than their pre-intervention baseline. In Experiment Two (n = 88), normative elements of the intervention were supplemented with a reminder notification prior to the onset of the simulated critical peak demand period. The results showed that, relative to the control condition, the 8-h notification reduced demand by 20% on average with a 12% decrease for the 24-h notification (with 2-h follow-up). These results indicate that peak energy issues can be alleviated using low-cost and easily implemented behaviour change strategies.
A Novel Urban Tourism Path Planning Approach Based on a Multiobjective Genetic Algorithm
Authors: Damos Mohamed A. , Zhu Jun , Li Weilian , Hassan Abubakr , Khalifa Elhadi
Published: August 2021

One of the most important variables that leads to effective individual and group tours is the tourism route planning approach, which enables tourists to engage with tourism with ease, speed, and safety. However, current methods of designing tourist routes have some glitches, such as relying only on external objectives to find the best route. In this paper, a novel urban tourism path planning method based on a multiobjective genetic algorithm is proposed. The main goal of this paper is to enhance the accuracy of the genetic algorithm (GA) by adopting new parameters and selecting the optimal tourism path by combining external and internal tourist site potentials. Moreover, the GA and analytical hierarchy process (AHP) were used in our proposed approach to evaluate urban tourism route planning under multiple conflicting objectives. To visualize and execute the proposed approach, the geographic information system (GIS) environment was used. Our suggested approach has been applied to develop the tourist road network of Chengdu City in China. Compared with existing tourism path planning approaches, our proposed approach is more accurate and straightforward than other approaches used to choose routes.
PocketCTF: A Fully Featured Approach for Hosting Portable Attack and Defense Cybersecurity Exercises
Authors: Karagiannis Stylianos , Ntantogian Christoforos , Magkos Emmanouil , Ribeiro Luís L. , Campos Luís
Published: August 2021

Capture the flag (CTF) challenges are broadly used for engaging trainees in the technical aspects of cybersecurity, maintaining hands-on lab exercises, and integrating gamification elements. However, deploying the appropriate digital environment for conducting cybersecurity exercises can be challenging and typically requires a lot of effort and system resources by educators. In this paper, we present PocketCTF, an extensible and fully independent CTF platform, open to educators to run realistic virtual labs to host cybersecurity exercises in their classrooms. PocketCTF is based on containerization technologies to minimize the deployment effort and to utilize less system resources. A proof-of-concept implementation demonstrates the feasibility of deploying CTF challenges that allows the trainees to engage not only in offensive security but also in defensive tasks that have to be conducted during cybersecurity incidents. When using PocketCTF, educators can deploy hands-on labs, spending less time on the deployment and without necessarily having the advanced technical background to deploy complex labs and scenarios.
Improving Ruby on Rails-Based Web Application Performance
Authors: Klochkov Denys , Mulawka Jan
Published: August 2021

The evolution of web development and web applications has resulted in creation of numerous tools and frameworks that facilitate the development process. Even though those frameworks make web development faster and more efficient, there are certain downsides to using them. A decrease in application performance when using an “off the shelf” framework might be a crucial disadvantage, especially given the vital role web application response time plays in user experience. This contribution focuses on a particular framework—Ruby on Rails. Once the most popular framework, it has now lost its leading position, partially due to slow performance metrics and response times, especially in larger applications. Improving and expanding upon the previous work in this field, an attempt to improve the response time of a specially developed benchmark application is made. This is achieved by performing optimizations that can be roughly divided into two groups. The first group concerns the frontend improvements, which include: adopting the client-side rendering, JavaScript Document Object Model (DOM) manipulation and asynchronous requests. Another group can be described as the backend improvements, which include implementing intelligent, granular caching, disabling redundant modules, as well as profiling and optimizing database requests and reducing database access inefficiencies. Those improvements resulted in overall up to 74% decreased page loading times, with perceived application performance being improved above this mark due to the adoption of a client-side rendering strategy. Using the different metrics of application performance measurements, each of the improvement steps is evaluated with regards to its effect on different aspects of overall performance. In conclusion, this work presents a way to significantly decrease the response time of a particular Ruby on Rails application and simultaneously provide a better user experience. Even though the majority of this process is specific to Rails, similar steps can be taken to improve applications implemented with the use of other similar frameworks. As the result of the work, a groundwork is laid for the development of the tool that could assist the developers in improving their applications as well.
Extensive Ossification of the Achilles Tendon with and without Acute Fracture: A Scoping Review
Authors: Sullivan Daniel , Pabich Allison , Enslow Ryan , Roe Avery , Borchert Donald , Barr Keenan , Cook Bailey , Brooks Amanda
Published: August 2021

Extensive Ossification of the Achilles Tendon (EOAT) is an uncommon condition characterized by the presence of heterotopic ossification within the substance of the Achilles Tendon and is distinct from other tendinopathies associated with tendon mineralization. The purpose of this scoping review of the literature on EOAT is to describe the pathogenesis, patient population, presentation, management, and outcomes of this rare condition. Fifty-four articles were included in the scoping review after screening and selection. According to the literature, EOAT often presents with pain and swelling around the Achilles Tendon and is frequently associated with acute trauma. EOAT is more common in men, and although the exact mechanisms of the pathology are not fully understood, EOAT may demonstrate specific molecular signaling patterns. The lack of knowledge regarding the molecular mechanism may be a significant hindrance to the management of the condition. Even though a standard treatment regimen for EOAT does not exist, conservative management for six months in patients without complications is recommended. Those who have an acute fracture of the ossification should be managed more aggressively and will often require surgical repair with autograft, although there is no standardized procedure at this time. Clinicians should be aware of the typical presentation, risk factors, and management options of patients with EOAT. Additionally, they should be cautious when selecting treatment strategies and conduct a thorough evaluation of long-term outcomes with various treatment modalities, which this review provides. Most important, this review highlights the need for further research to determine the best course of clinical treatment of EOAT injuries, in order to establish a standard treatment regimen.
H2S and Oxytocin Systems in Early Life Stress and Cardiovascular Disease
Authors: McCook Oscar , Denoix Nicole , Radermacher Peter , Waller Christiane , Merz Tamara
Published: August 2021

Today it is well established that early life stress leads to cardiovascular programming that manifests in cardiovascular disease, but the mechanisms by which this occurs, are not fully understood. This perspective review examines the relevant literature that implicates the dysregulation of the gasomediator hydrogen sulfide and the neuroendocrine oxytocin systems in heart disease and their putative mechanistic role in the early life stress developmental origins of cardiovascular disease. Furthermore, interesting hints towards the mutual interaction of the hydrogen sulfide and OT systems are identified, especially with regards to the connection between the central nervous and the cardiovascular system, which support the role of the vagus nerve as a communication link between the brain and the heart in stress-mediated cardiovascular disease.
Invasion and Metastasis as a Central Hallmark of Breast Cancer
Authors: Saha Trishna , Solomon Jonathan , Samson Abraham O. , Gil-Henn Hava
Published: August 2021

Hanahan and Weinberg introduced the “hallmarks of cancer” and typified essential biological abilities acquired by human cancer. Since then, a growing understanding of hallmark principles associated with breast cancer has assisted knowledge-based therapeutics development; however, despite the rapidly increasing number of targeted therapeutics, enduring disease-free responses for most forms of breast cancer is rare. Invasion and metastasis are the most defining feature of breast cancer malignancy and the leading cause of patient mortality. Hence, we propose a modified hallmarks model adapted to breast cancer, in which invasion and metastasis are shifted to the center of attention, thereby emphasizing it as a potentially superior therapeutic target. Although the scientific community highly appreciates the importance of the invasion and metastasis hallmark, as can be demonstrated by the growing number of publications on breast cancer metastasis, very few clinical trials concentrate on testing anti-metastasis inhibitors and even fewer trials focus on inhibitors for breast cancer metastasis. Here, we discuss the obstacles of applying research on invasion and metastasis therapeutics into the clinic and present current developments that could provide a potential solution to this dilemma.
Rationale for Polyclonal Intravenous Immunoglobulin Adjunctive Therapy in COVID-19 Patients: Report of a Structured Multidisciplinary Consensus
Authors: Coloretti Irene , Berlot Giorgio , Busani Stefano , De Rosa Francesco Giuseppe , Donati Abele , Forfori Francesco , Grasselli Giacomo , Mirabella Lucia , Tascini Carlo , Viale Pierluigi , Girardis Massimo
Published: August 2021

Introduction: Adjunctive therapy with polyclonal intravenous immunoglobins (IVIg) is currently used for preventing or managing infections and sepsis, especially in immunocompromised patients. The pathobiology of COVID-19 and the mechanisms of action of Ig led to the consideration of this adjunctive therapy, including in patients with respiratory failure due to the SARS-CoV-2 infection. This manuscript reports the rationale, the available data and the results of a structured consensus on intravenous Ig therapy in patients with severe COVID-19. Methods: A panel of multidisciplinary experts defined the clinical phenotypes of COVID-19 patients with severe respiratory failure and, after literature review, voted for the agreement on the rationale and the potential role of IVIg therapy for each phenotype. Due to the scarce evidence available, a modified RAND/UCLA appropriateness method was used. Results: Three different phenotypes of COVID-19 patients with severe respiratory failure were identified: patients with an abrupt and dysregulated hyperinflammatory response (early phase), patients with suspected immune paralysis (late phase) and patients with sepsis due to a hospital-acquired superinfection (sepsis by bacterial superinfection). The rationale for intravenous Ig therapy in the early phase was considered uncertain whereas the panelists considered its use in the late phase and patients with sepsis/septic shock by bacterial superinfection appropriate. Conclusion: As with other immunotherapies, IVIg adjunctive therapy may have a potential role in the management of COVID-19 patients. The ongoing trials will clarify the appropriate target population and the true effectiveness.
Rapid Evolutionary Adaptation in Response to Selection on Quantitative Traits
Authors: Stephan Wolfgang
Published: August 2021

Evolutionary adaptation after sudden environmental changes can occur very rapidly. The mechanisms facilitating rapid adaptation range from strong positive directional selection leading to large shifts in the allele frequencies at a few loci (selective sweeps) to polygenic selection causing small changes in allele frequencies at many loci. In addition, combinations of these two extreme mechanisms may also result in fast evolution. In recent years, following reports of new case studies of rapid adaptation, population genetic models have been proposed to explain these observations. In these models, the role of the major selective forces (positive directional and stabilizing selection) is highlighted as well as the genetic architecture of quantitative traits. Furthermore, the factors limiting the speed of adaptation are analyzed, in particular, the effects of random genetic drift and demography due to finite population size.