bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization of soybean chitinase genes induced by rhizobacteria involved in the defense against Fusarium oxysporum
Authors: Chen Jheng-Yan , Sang Hyunkyu , Chilvers Martin I. , Wu Chih-Hang , Chang Hao-Xun
Published: February 2024

Rhizobacteria are capable of inducing defense responses via the expression of pathogenesis-related proteins (PR-proteins) such as chitinases, and many studies have validated the functions of plant chitinases in defense responses. Soybean (Glycine max) is an economically important crop worldwide, but the functional validation of soybean chitinase in defense responses remains limited. In this study, genome-wide characterization of soybean chitinases was conducted, and the defense contribution of three chitinases (GmChi01, GmChi02, or GmChi16) was validated in Arabidopsis transgenic lines against the soil-borne pathogen Fusarium oxysporum. Compared to the Arabidopsis Col-0 and empty vector controls, the transgenic lines with GmChi02 or GmChi16 exhibited fewer chlorosis symptoms and wilting. While GmChi02 and GmChi16 enhanced defense to F. oxysporum, GmChi02 was the only one significantly induced by Burkholderia ambifaria. The observation indicated that plant chitinases may be induced by different rhizobacteria for defense responses. The survey of 37 soybean chitinase gene expressions in response to six rhizobacteria observed diverse inducibility, where only 10 genes were significantly upregulated by at least one rhizobacterium and 9 genes did not respond to any of the rhizobacteria. Motif analysis on soybean promoters further identified not only consensus but also rhizobacterium-specific transcription factor-binding sites for the inducible chitinase genes. Collectively, these results confirmed the involvement of GmChi02 and GmChi16 in defense enhancement and highlighted the diverse inducibility of 37 soybean chitinases encountering F. oxysporum and six rhizobacteria.
GhBRX.1, GhBRX.2, and GhBRX4.3 improve resistance to salt and cold stress in upland cotton
Authors: Wei Wei , Ju Jisheng , Zhang Xueli , Ling Pingjie , Luo Jin , Li Ying , Xu Wenjuan , Su Junji , Zhang Xianliang , Wang Caixiang
Published: February 2024

Introduction

Abiotic stress during growth readily reduces cotton crop yield. The different survival tactics of plants include the activation of numerous stress response genes, such as BREVIS RADIX (BRX).

Methods

In this study, the BRX gene family of upland cotton was identified and analyzed by bioinformatics method, three salt-tolerant and cold-resistant GhBRX genes were screened. The expression of GhBRX.1, GhBRX.2 and GhBRXL4.3 in upland cotton was silenced by virus-induced gene silencing (VIGS) technique. The physiological and biochemical indexes of plants and the expression of related stress-response genes were detected before and after gene silencing. The effects of GhBRX.1, GhBRX.2 and GhBRXL4.3 on salt and cold resistance of upland cotton were further verified.

Results and discussion

We discovered 12, 6, and 6 BRX genes in Gossypium hirsutum, Gossypium raimondii and Gossypium arboreum, respectively. Chromosomal localization indicated that the retention and loss of GhBRX genes on homologous chromosomes did not have a clear preference for the subgenomes. Collinearity analysis suggested that segmental duplications were the main force for BRX gene amplification. The upland cotton genes GhBRX.1, GhBRX.2 and GhBRXL4.3 are highly expressed in roots, and GhBRXL4.3 is also strongly expressed in the pistil. Transcriptome data and qRT‒PCR validation showed that abiotic stress strongly induced GhBRX.1, GhBRX.2 and GhBRXL4.3. Under salt stress and low-temperature stress conditions, the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and the content of soluble sugar and chlorophyll decreased in GhBRX.1-, GhBRX.2- and GhBRXL4.3-silenced cotton plants compared with those in the control (TRV: 00). Moreover, GhBRX.1-, GhBRX.2- and GhBRXL4.3-silenced cotton plants exhibited greater malondialdehyde (MDA) levels than did the control plants. Moreover, the expression of stress marker genes (GhSOS1, GhSOS2, GhNHX1, GhCIPK6, GhBIN2, GhSnRK2.6, GhHDT4D, GhCBF1 and GhPP2C) decreased significantly in the three target genes of silenced plants following exposure to stress. These results imply that the GhBRX.1, GhBRX.2 and GhBRXL4.3 genes may be regulators of salt stress and low-temperature stress responses in upland cotton.
A theory of the skill-performance relationship
Authors: Iso-Ahola Seppo E.
Published: February 2024

The skill-performance relationship is a cornerstone of a meritocratic society. People are selected for schools, colleges and jobs based on the premise that more skillful individuals perform better. Scientific understanding of the skill-performance relationship demands that the effect of skill on performance is objectively assessed without subjective, social, and political considerations. One of the best areas for this analysis is sports. In many sports settings, the skill-performance relationship can objectively be examined at the technical, behavioral, psychological, and neurological levels. This examination reveals that skill and performance are inextricably intertwined. While skill affects performance, performance in turn defines and affects skill. To disentangle the previously confusing and interchangeable use of these key constructs, the paper presents a theoretical model specifying that ability and effort have their own direct effects on performance, as well as indirect effects on performance through skill possession and skill execution in cognitive and physical domains of human performance. Thus, ability and skill are not the same. Although skill is a key determinant of performance, recent theory and research suggests that successful performers are successful not just because of their skills per se, but because they take advantage of their skills by creating more occurrences of momentum, making them last longer, and using them to bounce back faster from streaks of unsuccessful performance. Thus, momentum is an important mediator of the effects of skill on performance.
A generic approach to estimate airborne concentrations of substances released by indoor spray processes using a deterministic 2-box model
Authors: Hahn Stefan , Schwarz Katharina , Nowak Norman , Schwarz Janine , Meyer Jessica , Koch Wolfgang
Published: February 2024

Sprays are used both in workplace and consumer settings. Although spraying has advantages, such as uniform distribution of substances on surfaces in a highly efficient manner, it is often associated with a high inhalation burden. For an adequate risk assessment, this exposure has to be reliably quantified. Exposure models of varying complexity are available, which are applicable to spray applications. However, a need for improvement has been identified. In this contribution, a simple 2-box approach is suggested for the assessment of the time-weighted averaged exposure concentration (TWA) using a minimum of input data. At the moment, the model is restricted to binary spray liquids composed of a non-volatile fraction and volatile solvents. The model output can be refined by introducing correction factors based on the classification and categorization of two key parameters, the droplet size class and the vapor pressure class of the solvent, or by using a data set of experimentally determined airborne release fractions related to the used spray equipment. A comparison of model results with measured data collected at real workplaces showed that this simple model based on readily available input parameters is very useful for screening purposes. The generic 2-box spray model without refinement overestimates the measurements of the considered scenarios in approximately 50% of the cases by more than a factor of 100. The generic 2-box model performs better for room spraying than for surface spraying, as the airborne fraction in the latter case is clearly overestimated. This conservatism of the prediction was significantly reduced when correction factors or experimentally determined airborne release fractions were used in addition to the generic input parameters. The resulting predictions still overestimate the exposure (ratio tool estimate to measured TWA > 10) or they are accurate (ratio 0.5–10). If the available information on boundary conditions (application type, equipment) does not justify the usage of airborne release fraction, room spraying should be used resulting in the highest exposure estimate. The model scope may be extended to (semi)volatile substances. However, acceptance may be compromised by the limited availability of measured data for this group of substances and thus may have limited potency to evaluate the model prediction.
Physical activity interventions in workplace health promotion: objectives, related outcomes, and consideration of the setting—a scoping review of reviews
Authors: Schaller Andrea , Stassen Gerrit , Baulig Lukas , Lange Martin
Published: February 2024

Background

The workplace is a recognized setting for promoting health among adults, and physical activity (PA) interventions are an integral part of workplace health promotion (WHP).

Objectives

The present review of reviews aims to provide an overview of the main objectives and related outcomes addressed in WHP-related PA interventions, as well as the setting-specific aspects considered in the research field.

Methods

A scoping review of reviews was conducted. Reviews were included if they were peer-reviewed, written in English, and focused on PA interventions conducted in the context of WHP. A literature search was conducted in PubMed, SPORTDiscus, and Web of Science. Reviews were included if they had been published after the year 2000. Information on the following dimensions was extracted: author, region, number of primary studies included, target group(s), PA interventions included, main objective(s), related outcomes, and setting-specific aspects.

Results

A total of 17 reviews were included. Six reviews aimed at solely identifying the effectiveness of promoting daily PA and reducing sedentary behavior. Eleven reviews showed a combined approach considering physical activity behavior and/or health and job-related outcomes. Outcomes in the primary studies were heterogeneous. None of the reviews had an explicit definition of WHP and setting-specific information was very general and sparse. The reported setting-specific information was referred to the general importance of the workplace setting, the specific importance as an access route to target groups, and implementation aspects. Regarding the additional characteristics of the reviews, the selection of primary studies was restricted to a specific region in 2 of the 17 reviews in advance. Three reviews restricted the target group (sedentary workers, women, desk-based workers), while eleven reviews included working adults in general and, three reviews gave no information about the target group. Eleven intervention approaches of the reviews were behaviorally oriented, two focused solely on environmental interventions, and four reviews can be attributed to a combined approach considering behavioral and environmental interventions.

Conclusion

For sustainable future developments, the present results indicate a strong need for conceptual consolidation of WHP in the research field of PA interventions. Therefore, both WHP and health-related PA interventions need to take a comprehensive approach comprising behavioral and environmental interventions.
MAPKs signaling is obligatory for male reproductive function in a development-specific manner
Authors: Kumar Lokesh , Solanki Subhash , Jain Ashish , Botts Michael , Gupta Rahul , Rajput Sandeep , Roti Roti Elon
Published: February 2024

Mitogen-activated protein kinases (MAPKs) represent widely expressed and evolutionarily conserved proteins crucial for governing signaling pathways and playing essential roles in mammalian male reproductive processes. These proteins facilitate the transmission of signals through phosphorylation cascades, regulating diverse intracellular functions encompassing germ cell development in testis, physiological maturation of spermatozoa within the epididymis, and motility regulation at ejaculation in the female reproductive tract. The conservation of these mechanisms appears prevalent across species, including humans, mice, and, to a limited extent, livestock species such as bovines. In Sertoli cells (SCs), MAPK signaling not only regulates the proliferation of immature SCs but also determines the appropriate number of SCs in the testes at puberty, thereby maintaining male fertility by ensuring the capacity for sperm cell production. In germ cells, MAPKs play a crucial role in dynamically regulating testicular cell-cell junctions, supporting germ cell proliferation and differentiation. Throughout spermatogenesis, MAPK signaling ensures the appropriate Sertoli-to-germ cell ratio by regulating apoptosis, controlling the metabolism of developing germ cells, and facilitating the maturation of spermatozoa within the cauda epididymis. During ejaculation in the female reproductive tract, MAPKs regulate two pivotal events—capacitation and the acrosome reaction essential for maintaining the fertility potential of sperm cells. Any disruptions in MAPK pathway signaling possibly may disturb the testicular microenvironment homeostasis, sperm physiology in the male body before ejaculation and in the female reproductive tract during fertilization, ultimately compromising male fertility. Despite decades of research, the physiological function of MAPK pathways in male reproductive health remains inadequately understood. The current review attempts to combine recent findings to elucidate the impact of MAPK signaling on male fertility and proposes future directions to enhance our understanding of male reproductive functions.
Legal violence: the struggles of Thai women in Thai massage businesses
Authors: Kantachote Krittiya
Published: February 2024

Prior studies of legal violence concerning minority groups in the United States are often linked to Immigration Law. Drawing primarily from 30 interviews with Thai massage business owners in Los Angeles, this article reveals how the implementation of local and federal regulations and laws regarding massage businesses constitutes legal violence. Using Crenshaw’s structural, political, and representational intersectionality, this article demonstrates how Thai women’s race, ethnicity, and gender affect their experiences in countering the state regulations. The analysis focuses on two interrelated areas of Thai massage business owners’ and Thai massage therapists’ lives—the strict government surveillance of Thai massage business operations and the enforcement of professional certification. This legal violence toward Thai massage owners and massage therapists is rooted in a legal system that aims to protect consumers. Nevertheless, the effect of legal violence is not limited to undocumented Thai immigrants but also affects Thai American citizens and legal permanent residents.
Interactions between climate, shade canopy characteristics and cocoa production in Colombia
Authors: Hernández-Nuñez Héctor Eduardo , Suárez Juan Carlos , Andrade Hernán J. , Acosta José Ramiro Sánchez , Núñez Ramiro Duarte , Gutiérrez David Ricardo , Gutiérrez Gustavo Adolfo , Gutiérrez-Montes Isabel , Casanoves Fernando
Published: February 2024

Properly designed agroforestry systems (AFS) can generate optimal cocoa bean (BC) yields, produce co-products and provide ecosystem services. This study analyzes the interactions between climate, AFS structure and BC yield in six climatic zones across three natural regions of Colombia. A total of 305 plots of 1,000 m2 each were established in 132 farms where the cocoa-AFS structure, BC yield and climatic variables were determined. Five typologies of cocoa-AFS were obtained based on the characteristics of the shade canopy and the abundance of cocoa trees: “Highly diversified multistratum with high biomass” (HDMHB), “Diversified multistratum with high shade and abundance of Musaceae (DMHSM),” “Diversified multistratum with high abundance of cocoa trees (DMHDC),” “Diversified monostratum with low shade (DMLS)” and “monostratum with minimal shade (MMS).” In the departments of Huila and Caquetá, Andean and Amazonia regions, respectively, the HDMHB typology predominated, while in Meta, the Orinoquia region, it was MMS. In the temperate-humid zone, the DMHDC and DMHSM typologies were not found. A high floristic diversity of the shade canopy was found: 229 species; Caquetá registered the highest number (152). The most frequent canopy companion species were Musa paradisiaca, Cariniana pyriformis, Cedrela odorata, Psidium guajava, Musa sapientum, and Cordia alliodora. The highest abundance of cocoa trees occurs in areas with lower temperature and relative humidity and in AFS with lower abundance of fruit and timber trees. Zones with higher temperature and lower precipitation had higher abundance of timber species (r = 0.23). The BC yield is higher in areas with higher precipitation and is related to the lower abundance of individuals of timber and fruit species, and to the higher abundance of Fabaceae. The BC yield depends on the typology (p < 0.0001) of the cacao systems and was higher in DMHDC (1,148 kg ha−1 yr.−1). These results are key for the design of cocoa-AFS farms that maximize the integral production of BC, co-products and ecosystem services, approaching sustainable cocoa farming.
A review of robotic-assisted laparoscopic partial nephrectomy in the management of renal duplication anomalies
Authors: Batra Nikhil Varun , Dangle Pankaj
Published: February 2024

Open and purely laparoscopic partial nephrectomy or heminephrectomy has been the standard management for renal duplication anomalies for symptomatic children with non-functional renal moieties. While robotic-assisted laparoscopic partial nephrectomy (RALPN) has been established as a safe and feasible option for the management of renal duplex anomalies, there remains a paucity of data on this topic. The aim of this study is to comprehensively review all available outcomes data and update the use of emerging technologies in robotic surgery which continue to make RALPN a viable and advantageous option in the management of renal duplex anomalies.
Benzoic acid supplementation improves the growth performance, nutrient digestibility and nitrogen metabolism of weaned lambs
Authors: Zhang Wenjie , Sun Shuo , Zhang Yaqian , Zhang Yanan , Wang Jianguo , Liu Zhiqiang , Yang Kailun
Published: February 2024

Nitrogen is one of the essential components of proteins and nucleic acids and plays a crucial role in the growth and development of ruminants. However, the nitrogen utilization rate of ruminants is lower than that of monogastric animals, which not only reduces protein conversion and utilization, but also increases manure nitrogen discharge as well as causing environmental pollution. The lamb stage is an important period in the life of sheep, which can affect the production performance and meat quality of fattening sheep. The purpose of this experiment was to explore effects of benzoic acid supplementation on growth performance, nutrient digestibility, nitrogen metabolism and plasma parameters of weaned lambs. A total of 40 weaned male Hu sheep lambs with similar body weight were randomly divided into 4 groups: control with no benzoic acid (0 BA) and the lambs in other 3 groups were fed 0.5, 1, and 1.5% benzoic acid on the basis of experimental diet (0.5, 1, and 1.5 BA, respectively). The experiment lasted for 60 days. Results showed that the average daily gain of 1 BA group was significantly increased (p < 0.05) when compared to 0 and 1.5 BA groups, while an opposite tendency of dry matter intake to average daily gain ratio was observed. The dry matter, organic matter, neutral detergent fiber and acid detergent fiber digestibility of 1 BA group was significantly increased (p < 0.05) as compared with 0 and 1.5 BA groups as well as plasma albumin content. Also, the urinary hippuric acid and hippurate nitrogen concentrations in 1 and 1.5 BA groups were higher (p < 0.05) than those in 0 and 0.5 BA groups. Additionally, the nitrogen intake in 0.5 and 1 BA groups was significantly increased (p < 0.05) when compared to other groups. At 1 h after morning feeding, the plasma benzoic acid concentration of 1 BA group reached up to maximum value and was higher (p < 0.05) than other groups, and then began to decrease. Similarly, the hippuric acid concentration in plasma of 1 and 1.5 BA groups was higher (p < 0.05) than that of 0 BA group from 1 to 4 h post morning feeding. At 3 h after feeding, the urea nitrogen concentration in plasma of 0 BA group was higher (p < 0.05) than that of 1.5 BA group. Overall, the appropriate supplementation of benzoic acid (1%) in the diet can improve growth performance and nitrogen metabolism of weaned lambs.
Characterizing Trends in the Use of Food Donations and Other Food-Related Community-Based Social Assistance Programs in a Cohort of New Food Bank Users in Quebec, Canada
Authors: Pérez Elsury Johanna , Carabali Mabel , Mercille Geneviève , Sylvestre Marie-Pierre , Roncarolo Federico , Potvin Louise
Published: February 2024

Objective:To characterize 12-month trends in the use of food donations and other food-related community-based social assistance programs (CB-SAPs) during the first year following the enrollment of new food bank (FB) users in Quebec, Canada.

Methods:A cohort of 1,001 newly registered FB-users in Quebec from the Pathways Study were followed-up during 12-month following baseline assessment. Outcomes were monthly use of food donations and other food-related CB-SAPs. Main predictors were alternative food source utilization (AFSU) profiles: 1) exclusive-FB-users; 2) FB+fruit/vegetable-market-users; and 3) Multiple/diverse-AFS-users. Covariates included sociodemographic characteristics, health status, and major life events. We fit Bayesian hierarchical mixed-effect models, accounting for spatial clustering, temporal correlation, and censoring.

Results:We observed an overall downward trend of food donation use among study completers (n= 745). Each AFSU profile had a distinctive monthly trend of food donation use, but probabilities of use across the three profiles overlapped, between 44% and 55%. The use of other food-related CB-SAPs was low and not correlated with AFSU profiles.

Conclusion:De novoFB-users use food donations in different ways over time according to specific contextual AFSU profiles.
Management of Neuroendocrine Breast Carcinoma (NEBC): Review of Literature
Authors: Mohamed A. , Zeidalkilani J. , Asa S. L. , Trybula M. , Montero Alberto J.
Published: February 2024

Extra pulmonary high-grade poorly differentiated neuroendocrine carcinomas (EP-NECs) are rare tumors that usually arise in the gastrointestinal and genitourinary tracts. Primary neuroendocrine carcinoma of the breast (NEBC) is extremely rare, representing less than 0.1% of all breast cancers and less than 1% of neuroendocrine neoplasms. Consequently, they can be misdiagnosed as other types of breast cancer, however, proper immunohistochemical (IHC) studies can assist with making the correct diagnosis. Management of NEBC can be challenging given the paucity of evidence-based literature and should not routinely follow the therapeutic guidelines of other breast cancers. In this article, we review the current literature regarding the management of NEBC.
Development of a Detection System for Types of Weeds in Maize (Zea mays L.) under Greenhouse Conditions Using the YOLOv5 v7.0 Model
Authors: García-Navarrete Oscar Leonardo , Santamaria Oscar , Martín-Ramos Pablo , Valenzuela-Mahecha Miguel Ángel , Navas-Gracia Luis Manuel
Published: February 2024

Corn (Zea mays L.) is one of the most important cereals worldwide. To maintain crop productivity, it is important to eliminate weeds that compete for nutrients and other resources. The eradication of these causes environmental problems through the use of agrochemicals. The implementation of technology to mitigate this impact is also a challenge. In this work, an artificial vision system was implemented based on the YOLOv5s (You Only Look Once) model, which uses a single convolutional neural network (CNN) that allows differentiating corn from four types of weeds, for which a mobile support structure was built to capture images. The performance of the trained model had a value of mAP@05 (mean Average Precision) at a threshold of 0.5 of 83.6%. A prediction accuracy of 97% and a mAP@05 of 97.5% were obtained for the maize class. For the weed classes, Lolium perenne, Sonchus oleraceus, Solanum nigrum, and Poa annua obtained an accuracy of 86%, 90%, 78%, and 74%, and a mAP@05 of 81.5%, 90.2%, 76.6% and 72.0%, respectively. The results are encouraging for the construction of a precision weeding system.
An Evaluation of Bacterial Wilt (Ralstonia solanacearum) Resistance in a Set of Tomato Germplasm from the United States Department of Agriculture
Authors: Phiri Theresa Makawa , Bhattarai Gehendra , Chiwina Kenani Edward , Fan Qiurong , Xiong Haizheng , Alatawi Ibtisam , Dickson Ryan , Joshi Neelendra K. , Rojas Alejandro , Ling Kai-Shu , Shi Ainong
Published: February 2024

Bacterial wilt (BW), caused by Ralstonia solanacearum, is one of the devastating diseases in tomatoes (Solanum lycopersicum L.). The use of resistant cultivars and breeding for genetic resistance is the most effective, economical, and environmentally friendly management strategy for this disease. It is necessary to screen diverse germplasm and cultivated genotypes to identify resistant resources and to develop resistant cultivars in tomatoes to combat the changing pathogen isolates. This study evaluated 40 United States Department of Agriculture (USDA) tomato accessions for their BW resistance to the R. solanacearum isolate P822 under greenhouse conditions. The tomato plants were inoculated and visually assessed to observe their symptoms, and the disease severity was scored on a scale of 0 to 4 (0 = no leaf wilted, 1 = 25% of leaves wilted, 2 = 50% leaves wilted, 3 = 75% of leaves wilted, and 4 = 100% leaves wilted). Five accessions (PI 645370, PI 647306, PI 600993, PI 355110, and PI 270210) were observed as BW resistance, with PI 645370 showing the greatest resistance. The broad-sense heritability for BW resistance was estimated as 59.9% and 42.8% based on a 0–4 scale of disease incidence and the disease severity index, respectively. Two distinct clusters (sub-populations) were detected among 39 of the 40 accessions. The five identified BW-resistant accessions were distributed in both clusters, suggesting a likely difference in the genetic base among the five resistance accessions. The resistant accessions will contribute significantly to the tomato breeding program to develop new cultivars with BW resistance.
Enhancement of Soil Organic Carbon, Water Use Efficiency and Maize Yield (Zea mays L.) in Sandy Soil through Organic Amendment (Grass Peat) Incorporation
Authors: Yang Kaiqi , Hu Jian , Ren Yunzhuo , Zhang Zhiao , Tang Mei , Shang Zhenkun , Zhen Qing , Zheng Jiyong
Published: February 2024

The efficient use of organic amendment (OM) is considered an economic, environmental and sustainable practice to improve soil quality, especially the accumulation of organic carbon (C) and water use efficiency (WUE) in dryland agriculture. However, the effect of different OM on soil nutrients, organic carbon fractions, water content and maize yield is unclear in arid and semi-arid regions with sandy soil. Field experiments with four OM, grass peat (GP), biochar (BC), organic fertilizer (OF) and maize straw (MS), were conducted with an equivalent amount of C input on the southeastern edge of Mu Us Sandy Land in China. Results indicated that the soil nutrients and labile organic carbon (DOC, MBC, KMnO4-C and POC) concentrations were higher under OM (GP, BC, OF and MS) treatments than in CK in the 0–0.10 m soil layers. GP treatment remarkably improved carbon pool index values (1.63, 2.51 and 2.24, respectively) in all layers compared to CK (1.00). At maturity stages of maize, the soil water content (SWC) under GP and OF treatments (11.3–13.4%) was remarkably higher than that in CK treatment (around 10.0%). Yield and WUE were remarkably greater in GP and OF treatments compared to CK. The results proved that GP amendment is superior for barren sandy soil than BC, OF and MS treatments in improving soil nutrients, organic carbon sequestration, WUE and crop yield in China.
The Role of Endogenous Brassinosteroids in the Mechanisms Regulating Plant Reactions to Various Abiotic Stresses
Authors: Miao Rong , Li Caijuan , Liu Ziliang , Zhou Xiangyan , Chen Sijin , Zhang Dan , Luo Jiaqi , Tang Wenhui , Wang Cuiling , Wu Jiling , Chen Zhengjun
Published: February 2024

Plants are vulnerable to many abiotic stresses, resulting in reduced plant productivity. Its adaptation to unfavorable environments relies on transmitting external stress signals into internal signaling pathways. A series of stress response mechanisms have been developed. Among them, brassinosteroids (BRs) are a class of steroid hormones that are widely involved in plant growth, development, and stress response. Via genetics, proteomics, and genomics studies, the major components of signaling and signaling pathways through a series of phosphorylation cascade reactions have been identified in model plants such as Arabidopsis. Numerous studies have shown that BRs play important roles in plant responses to drought, temperature, salt, heavy metals, and other environmental stresses. The application of BRs to improve plant stress resistance has become the focus of research in recent years, especially the regulation of stress via endogenous BRs. Therefore, this paper systematically summarizes the research progress related to endogenous BR levels and provides an overview of BR biosynthesis and the signaling pathway, as well as the function of endogenous BRs in the response to abiotic stresses.
Exploring Microbial Rhizosphere Communities in Asymptomatic and Symptomatic Apple Trees Using Amplicon Sequencing and Shotgun Metagenomics
Authors: Muñoz-Ramírez Zilia Y. , González-Escobedo Román , Avila-Quezada Graciela D. , Ramírez-Sánchez Obed , Higareda-Alvear Victor M. , Zapata-Chávez Emiliano , Borrego-Loya Alejandra , Muñoz-Castellanos Laila N.
Published: February 2024

The rhizosphere is a dynamic and highly interactive habitat where diverse microbial communities are established, and it plays crucial roles in plant health and disease dynamics. In this study, microbial communities and functional profiles in the rhizosphere of both asymptomatic and symptomatic apple trees were investigated through amplicon sequencing and shotgun metagenomics. The research was conducted at a location in the municipality of Cuauhtemoc, Chihuahua State, Mexico, and a total of 22 samples were collected, comprising 12 for amplicon sequencing and 10 for shotgun metagenomic sequencing. Symptomatic trees were identified based on reddish branches and internal necrosis in the trunk and root, while asymptomatic trees exhibited a healthy physiology. The findings showed that the dominant bacterial phyla included Proteobacteria, Actinobacteria, and Bacteroidetes, with prevalent genera such as Streptomyces, Pseudomonas, and Rhodanobacter. The fungal communities featured Ascomycota, Mortierellomycota, and Basidiomycota, which were dominated by Fusarium, Penicillium, and Mortierella. In the fungal communities, Mortierellomycota, notably abundant in asymptomatic trees, holds potential as a biocontrol agent, as seen in other studies on the suppression of Fusarium wilt disease. The application of shotgun metagenomic sequencing revealed significant differences in alpha and beta diversities in bacterial communities, suggesting a health-dependent change in species composition and abundance. Functional profile analysis highlighted enzymatic activities associated with lipid synthesis/degradation, amino acid biosynthesis, carbohydrate metabolism, and nucleotide synthesis, which have been documented to participate in symbiotic relationships between plants. These insights not only contribute to understanding the dynamics of rhizosphere microbial activity but also provide valuable perspectives on the potential application of microbial communities for tree health and implications for the management of apple orchards.
Lactiplantibacillus plantarum and Saccharomyces cerevisiae—Fermented Coconut Water Alleviates Dextran Sodium Sulfate-Induced Enteritis in Wenchang Chicken: A Gut Microbiota and Metabolomic Approach
Authors: Zheng Leijie , Han Zhe , Zhang Jiachao , Kang Jiamu , Li Congfa , Pang Qing , Liu Sixin
Published: February 2024

In order to investigate the potential mechanisms of probiotic-fermented coconut water in treating enteritis, this study conducted a comprehensive analysis of the effects of probiotic intervention on the recovery from Dextran Sodium Sulfate-induced acute enteritis in Wenchang chicks. The analysis encompassed the assessment of growth performance, serum indicators, intestinal tissue structure, and metagenomic and metabolomic profiles of cecal contents in 60 Wenchang chicks subjected to intervention. This approach aimed to elucidate the impact of probiotic intervention on the recovery process from acute enteritis at both the genetic and metabolic levels in the avian model. The results revealed that intervention with Saccharomyces cerevisiae Y301 improved the growth rate of chicks. and intervention with Lactiplantibacillus plantarum MS2c regulated the glycerophospholipid metabolism pathway and reshaped the gut microbiota structure in modeling chicks with acute enteritis, reducing the abundance of potentially pathogenic bacteria from the Alistipes and increasing the abundance of potentially beneficial species from the Christensenellaceae. This intervention resulted in the production of specific gut metabolites, including Gentamicin C and polymyxin B2, recognized for their therapeutic effects on acute enteritis. The combined intervention of S. cerevisiae Y301 and L. plantarum MS2c not only enhanced growth performance but also mitigated intestinal wall damage and increased the abundance of gut metabolites such as gentamicin C and polymyxin B2, thereby mitigating symptoms of enteritis. Furthermore, this combined intervention reduced the levels of serum immune markers, including IL-10, IL-6, TNF-α, IFN-γ, and D-lactic acid, thus mitigating intestinal epithelial cell damage and promoting acute enteritis recovery. This study provides crucial insights into the mechanisms of action of probiotics and probiotic-fermented coconut water in acute enteritis recovery, offering new perspectives for sustainable farming practices for Wenchang chicken.
Transcriptome Data Revealed the circRNA–miRNA–mRNA Regulatory Network during the Proliferation and Differentiation of Myoblasts in Shitou Goose
Authors: Huang Rongqin , Chen Jiahui , Dong Xu , Zhang Xiquan , Luo Wen
Published: February 2024

CircRNA, a recently characterized non-coding RNA (ncRNA) variant, functions as a molecular sponge, exerting regulatory control by binding to microRNA (miRNA) and modulating the expression of downstream proteins, either promoting or inhibiting their expression. Among poultry species, geese hold significant importance, prized by consumers for their delectable taste and rich nutritional content. Despite the prominence of geese, research on the growth and development of goose muscle, particularly the regulatory role of circRNAs in goose muscle formation, remains insufficiently explored. In this study, we constructed comprehensive expression profiles of circRNAs and messenger RNAs (mRNAs) within the myoblasts and myotubes of Shitou geese. We identified a total of 96 differentially expressed circRNAs (DEcircRNAs) and 880 differentially expressed mRNAs (DEmRNAs). Notably, the parental genes of DEcircRNAs and DEmRNAs exhibited enrichment in the Wnt signaling pathway, highlighting its potential impact on the proliferation and differentiation of goose myoblasts. Employing RNAhybrid and miRDB, we identified circRNA-miRNA pairs and mRNA-miRNA pairs that may play a role in regulating myogenic differentiation or muscle growth. Subsequently, utilizing Cytoscape, we constructed a circRNA–miRNA–mRNA interaction network aimed at unraveling the intricate regulatory mechanisms involved in goose muscle growth and development, which comprises 93 circRNAs, 351 miRNAs, and 305 mRNAs. Moreover, the identification of 10 hub genes (ACTB, ACTN1, BDNF, PDGFRA, MYL1, EFNA5, MYSM1, THBS1, ITGA8, and ELN) potentially linked to myogenesis, along with the exploration of their circRNA–miRNA–hub gene regulatory axis, was also conducted. These competitive endogenous RNA (ceRNA) regulatory networks elucidate the molecular regulatory mechanisms associated with muscle growth in Shitou geese, providing deeper insights into the reciprocal regulation of circRNA, miRNA, and mRNA in the context of goose muscle formation.
Treatment of Medial Instability of the Carpometacarpal and Tarsometatarsal Joints Using the Isolock® System in Two Dogs
Authors: Pinna Stefania , Tassani Chiara , Di Benedetto Matteo
Published: February 2024

This case report describes a novel procedure using the Isolock Intrauma® implant system for treating medial instability of the carpometacarpal and tarsometatarsal joints, as demonstrated in in two dogs. A 9-year-old spayed female Spanish greyhound presented with a non-weight-bearing right hindlimb following a trauma. The clinical and radiological findings confirmed medial tarsometatarsal instability consistent with valgus deviation of the tarsus and the opening of the joint line on the medial aspect from the first to the third tarsometatarsal joints. A 4-year-old female Drahthaar presented with a non-weight-bearing left forelimb, swelling of the carpus and valgus instability. Radiological examination revealed a widening of the spaces between the intermedioradial carpal bone, second carpal bone and metacarpal bone II, confirming the medial carpometacarpal instability. In both cases, the Isolock system, an implant including ultra-high-molecular-weight polyethylene suture (UHMWPE), was used to reinforce the medial joint structures. Minor short-term complications were observed, such as swelling of the tarsal surgical site and hyperextension of the carpus, but these resolved spontaneously. No lameness or major complications were reported five months postoperatively. Carpometacarpal and tarsometatarsal instabilities are rare diseases in dogs as compared to subluxations of the other joints of the carpus and tarsus. There are no previous reports regarding the use of a UHMPWE implant for the treatment of these rare joint injuries, though the present case report suggests the validity and efficacy of the Isolock Intrauma® implant for restoring carpal and tarsal stability and preserving joint mobility.
Antimicrobial Tolerance in Salmonella: Contributions to Survival and Persistence in Processing Environments
Authors: Obe Tomi , Kiess Aaron S. , Nannapaneni Ramakrishna
Published: February 2024

Salmonella remains a top bacterial pathogen implicated in several food-borne outbreaks, despite the use of antimicrobials and sanitizers during production and processing. While these chemicals have been effective, Salmonella has shown the ability to survive and persist in poultry processing environments. This can be credited to its microbial ability to adapt and develop/acquire tolerance and/or resistance to different antimicrobial agents including oxidizers, acids (organic and inorganic), phenols, and surfactants. Moreover, there are several factors in processing environments that can limit the efficacy of these antimicrobials, thus allowing survival and persistence. This mini-review examines the antimicrobial activity of common disinfectants/sanitizers used in poultry processing environments and the ability of Salmonella to respond with innate or acquired tolerance and survive exposure to persists in such environments. Instead of relying on a single antimicrobial agent, the right combination of different disinfectants needs to be developed to target multiple pathways within Salmonella.
Supplementation with Silybum marianum Extract, Synbiotics, Omega-3 Fatty Acids, Vitamins, and Minerals: Impact on Biochemical Markers and Fecal Microbiome in Overweight Dogs
Authors: Balouei Fatemeh , Stefanon Bruno , Martello Elisa , Atuahene David , Sandri Misa , Meineri Giorgia
Published: February 2024

Overweight and obese dogs can develop metabolic dysfunction, characterized by an inflammatory response and involvement of liver functions. If a modulation of the gut microbiome and its interaction with the gut–liver axis is implicated in the development of metabolic dysfunction, exploration becomes necessary. Over the past decade, diverse therapeutic approaches have emerged to target pathogenic factors involved in metabolic dysfunction. This study investigated the impact of a supplement with hepatoprotective activity, containing extracts of Silybum marianum, prebiotics, probiotics, n-3 polyunsaturated fatty acids, vitamins, and minerals on hematological markers of liver functions and inflammation, as well as on the intestinal microbiota of 10 overweight adult dogs over a 35-day time span. Animals underwent clinical and laboratory evaluations every 7 days, both before the administration of the supplement (T0) and after 7, 14, 21, 28, and 35 days (T1, T2, T3, T4, and T5). In comparison to T0, a significant (p < 0.05) decrease in ALP, glucose, direct bilirubin, and CRP was observed from T3 to T5. The alpha diversity of the fecal microbiota significantly decreased (p < 0.05) only at T1, with high variability observed between dogs. Total short-chain fatty acid and lactic acid were also lower at T1 (p < 0.05) compared to the other times of sampling. The beta diversity of the fecal microbiota failed to show a clear pattern in relation to the sampling times. These results of blood parameters in overweight dogs show a reduction of the inflammation and an improvement of metabolic status during the study period, but the effective contribution of the supplement in this clinical outcome deserves further investigation. Furthermore, the considerable individual variability observed in the microbiome hinders the confident detection of supplement effects.
Demography and Genealogical Analysis of Massese Sheep, a Native Breed of Tuscany
Authors: Giuliotti Lorella , Benvenuti Maria Novella , Preziuso Giovanna , Ventura Emilia , Fresi Pancrazio , Cecchi Francesca
Published: February 2024

This study investigates the genealogical and demographic trends of the Massese sheep breed in Tuscany from 2001 to 2021. The Herd Book kept by the Italian Sheep and Goat Breeders Association (Asso.Na.Pa) provided the data. The descriptive statistics were analyzed using JMP software. The pedigree parameters of a total of 311,056 animals (whole population—WP) were analyzed using CFC, ENDOG, and Pedigree viewer software. A total of 24,586 animals born in the period 2007–2021 represented the Reference Population (RP), and 18,554 animals the Base Population (BP). The demographic results showed an inconsistent trend of offspring registration. This study showed a short period of productivity for both ewes and rams, with means of 1.47 and 19.2 registered newborn ewes and rams, respectively. The genealogical analysis revealed incomplete data, highlighting inaccurate assessments of the relationships among the animals, and inbreeding with large differences among provinces. The average inbreeding coefficient in the WP was 1.16%, and it was 2.26% in the RP. The total number of inbreds was 2790 in the WP, with an average FPED of 13.56%, and 2713 in the RP, with an average FPED of 12.82%. The use of pedigree data is a key and economical approach to calculating inbreeding and relationship coefficients. It is the primary step in genetic management, playing a crucial role in the preservation of a breed. The regular updating of genealogical data is the first step to ensuring the conservation of animal genetic resources, and this study is compromised by the lack of such updates.
Finding Ways to Fight Antimicrobial Resistance: Present, Future, and Perspectives
Authors: Straniero Valentina
Published: February 2024

Antimicrobial resistance (AMR) is a worldwide severe topic, affecting both human and animal health [...]
Trends in Antibiotic Use in a Large Children’s Hospital in London (United Kingdom): 5 Years of Point Prevalence Surveys
Authors: Meesters Kevin , Chappell Faye , Demirjian Alicia
Published: February 2024

Background: Antibiotics are commonly prescribed in paediatrics. As their excessive use contributes to adverse drug events, increased healthcare costs, and antimicrobial resistance, antimicrobial stewardship initiatives are essential to optimising medical care. These single-centre point prevalence surveys aimed to provide insights into antibiotic prescribing trends and identify targets for paediatric AMS activities. Methods: 14 point prevalence surveys were conducted from March 2016–April 2021, collecting data on antibiotic prescriptions, indication, adherence to guidelines, and route of administration. The UK adapted the World Health Organisation’s AWaRe classification-guided antibiotic categorization. Results: 32.5% of all inpatients were on at least one antimicrobial; this remained stable during all surveys (range: 20–44%, p = 0.448). Of all prescriptions, 67.2% had an end- or review-date, and the majority was for agents in the Watch category (46.8–70.5%). Amoxicillin and clavulanate were the most frequently prescribed antibiotics (20.8%), followed by gentamicin (15.3%). Approximately 28.8% of all prescriptions were for prophylactic indications, while 7.6% of the prescriptions were not adherent to the hospital guidelines. Conclusions: This study highlights the importance of ongoing monitoring and robust AMS initiatives to ensure prudent antibiotic prescribing in paediatric healthcare. It underscores the need for tailored guidelines, educational efforts, and targeted interventions to enhance the quality of antibiotic usage, ultimately benefiting both individual patients and public health.
Occurrence of Trace-Level Antibiotics in the Msunduzi River: An Investigation into South African Environmental Pollution
Authors: Addis Temesgen Zelalem , Adu Joy Tuoyo , Kumarasamy Muthukrishnavellaisamy , Demlie Molla
Published: February 2024

The presence of antibiotics in the environmental matrix has raised concerns regarding their risk to the aquatic ecosystem and human health. Surface water, such as rivers, plays a pivotal role in the dispersion and transport of antibiotic residues. The effective monitoring of these contaminants requires investigating their sources and distribution. While numerous studies have been conducted globally to comprehend the emergence, prevalence, and management of these substances, the investigation of therapeutic antibiotics in Africa remains notably underrepresented. Consequently, data regarding these emerging contaminants in the African aquatic environments are scarce, warranting further exploration. This study aims to investigate the occurrence of four specific therapeutic antibiotics—tetracycline, sulfathiazole, penicillin g, and erythromycin—across different seasons in the Msunduzi River, Eastern South Africa. Three sampling campaigns were conducted during spring, autumn, and winter to assess the presence of these antibiotics in the river. Analyte extraction from water samples was achieved through solid-phase extraction, and quantification was performed using liquid chromatography–mass spectrometry. The findings reveal notable concentrations of these antibiotics in the river at locations closest to a wastewater treatment discharge point. Among the antibiotics studied, tetracycline (158.42–1290.43 ng/L) and sulfathiazole (112.68–1151.25 ng/L) were the most frequently detected compounds across the majority of the sampling sites and tributaries of the river. Erythromycin was less frequently detected in the surface water and wastewater effluent but was found to be a risk to algal species within the river. While wastewater effluents represent a significant source of antibiotic contamination in the river, tributaries from industrial areas and informal settlements were identified as continuous sources of antibiotic pollution. Thus, it is imperative to implement appropriate monitoring protocols to mitigate antibiotic pollution in the aquatic environment.