bluuebunny/crossref_metadata_2025 huggingface dataset:
Ultrasound and Sialendoscopy Findings in Radioactive Iodine–Induced Sialadenitis: Comparative Analysis and Possible Impact on Management
Authors: Koch Michael , Müller Sarina , Mantsopoulos Konstantinos , Iro Heinrich , Sievert Matti
Published: January 2024

Background: To assess the correlation/association between ultrasound and sialendoscopy findings in radioactive iodine therapy–induced sialadenitis (RAIS). Methods: Patients presenting with RAIS were investigated with ultrasound and sialendoscopy. Four pathologic ultrasound parameters and seven pathologic sialendoscopy parameters were retrospectively assessed. Correlations/associations between ultrasound and sialendoscopy findings and associations between the changes between the first and last ultrasound and sialendoscopy findings were assessed separately for the parotid (PG) and submandibular glands (SMG). Results: Sixty-seven patients were included. In the first examination, 107 glands were investigated (PGs 88.8%, SMGs 11.21%), and in the last examination, 64 glands were investigated (90.6% PGs, 9.4% SMGs). Highly significant positive associations were observed between the severity or category of ultrasound and sialendoscopy findings for first and last examinations for PGs (both p = 0.0001) and SMGs (p = 0.002; p = 0.037). Duct dilation had a significant negative association with the sialendoscopy findings for PGs in the first and last examinations (both p = 0.0001), but not for SMGs. Comparison of changes in the ultrasound and sialendoscopy findings between the first and last examinations showed a significant positive association for PGs (p = 0.0001) but not for SMGs. Conclusions: Ultrasound and sialendoscopy findings for the parenchyma and duct system in RAIS showed significant associations/correlations and can be useful for effective management in RAIS.
Improved YOLOv5 Algorithm for Real-Time Prediction of Fish Yield in All Cage Schools
Authors: Wang Lei , Chen Ling-Zhi , Peng Bo , Lin Ying-Tien
Published: January 2024

Cage aquaculture makes it easier to produce high-quality aquatic products and allows full use of water resources. 3Therefore, cage aquaculture development is highly valued globally. However, the current digitalization level of cage aquaculture is low, and the farming risks are high. Research and development of digital management of the fish population in cages are greatly desired. Real-time monitoring of the activity status of the fish population and changes in the fish population size in cages is a pressing issue that needs to be addressed. This paper proposes an improved network called CC-YOLOv5 by embedding CoordConv modules to replace the original ConV convolution modules in the network, which improves the model’s generalization capability. By using two-stage detection logic, the target detection accuracy is enhanced to realize prediction of the number of fish populations. OpenCV is then used to measure fish tail lengths to establish growth curves of the fish and to predict the output of the fish population in the cages. Experimental results demonstrate that the mean average precision (mAP) of the improved algorithm increases by 14.9% compared to the original YOLOv5, reaching 95.4%. This research provides an effective solution to promote the intelligentization of cage aquaculture processes. It also lays the foundation for AI (Artificial Intelligence) applications in other aquaculture scenarios.
Beach Litter Variability According to the Number of Visitors in Cádiz Beaches, SW Spain
Authors: Fernández García Gonzalo , Asensio-Montesinos Francisco , Anfuso Giorgio , Arenas-Granados Pedro
Published: January 2024

The amount and composition of litter was evaluated during May and June 2021 at two urban beaches, i.e., La Victoria and La Cortadura, in Cádiz, SW Spain. Surveys were carried out daily in the morning and in the evening during the weekends to quantify the daily accumulation of beach litter and relate it to the number of beach users, which was assessed at around 1:00 p.m. Litter amount was also related to cleanup operations that were very mechanically and manually carried out each day very early in the morning. A total of 8108 items were collected at the two investigated sectors during the study period and beach visitors were quantified in 22 surveys. Plastic was the most common material, representing 82% in La Victoria and 68% in La Cortadura. The most common items were cigarette butts and small, hard plastic fragments. Some litter items that were hazardous to beach visitors were identified, such as broken glass. The number of visitors was positively related to the amount of litter. Significant differences were seen in the litter abundance between the morning and evening assessments since the beaches were cleaned daily and bins were available to facilitate trash disposal. Cleaning operations remove many of the litter items but always leave small quantities of small items uncollected. Efforts to prevent litter on these beaches should focus on informing visitors properly in order to avoid littering and on improving cleanup operations.
The Impact of Household Dynamics on Land-Use Change in China: Past Experiences and Future Implications
Authors: Luo Yaxue , Chen Ruishan , Xiong Bo , Jia Nan , Guo Xiaona , Yin Chenglong , Song Wen
Published: January 2024

Population is the main driver of land-system and environmental change. However, population is usually treated as a variable that only considers the population number, and multi-dimensional population structure is largely ignored. There has been a systematic transition of population structure in the past several decades, including changes in household structure, increasing aging populations, increasing divorce rates, and increasing human migration. All of these changes have direct or indirect impacts on land use and environmental issues. Here, taking China as an example and using statistical analysis, namely the Mann–Kendall trend test and a land-use-transition matrix, we examine the relationship between household dynamics and land-use change in China by examining changes in household structure and land-use changes in China between 1980 and 2020. The results show the following three groups of findings. (1) The number of households increased by 130.95% from 1980 to 2020, while the population only increased by 42.83%; the size of households decreased from 4.41 to 2.62 in China from 1982 to 2020; and the household dynamics varied from province to province, which is affected by urbanization rate and economic development. (2) Birth rates, divorce rates, population aging, and migration all affect household structure, which directly or indirectly affect changes in land-use systems. (3) The changes in China’s land use are interlinked and interact with changes in household structure, which is evident in the increase in Residential land use and the abandonment of arable land. The rising household number increased the area of urban and rural settlement, leading to the fragmentation of cultivated land and the reduction of ecological land. To regulate land-use change for sustainable development, future land-use planning should take into account the effect of household dynamics and should reduce the negative effects of household dynamics on land systems and environmental change.
The Effect of d10 Precious Elements on Structural, Magnetic and Elastic Properties of MnPt Alloy: A First-Principles Study
Authors: Diale Ramogohlo , Ngoepe Phuti , Chauke Hasani , Moema Joseph , Phasha Maje
Published: January 2024

MnPt’s exceptional stability and extremely high Néel temperature have generated a lot of interest in data storage applications. Previously, it was reported experimentally that the MnPt alloy shows ferromagnetic (FM) behavior at room temperature. In this study, the effects of partial substitution of Pt with Pd, Au, and Ag on magnetic properties is investigated using density functional theory. The stability of Mn50Pt50−xMx (M = Pd, Au, Ag, x = 6.25, 12.5, 18.75) alloys was assessed by determining their thermodynamic, magnetic, and mechanical properties. The calculated lattice constants of Mn50Pt50 agree well with available theoretical results. The Mn50Pt50−xMx alloys’ formability was assessed by measuring the thermodynamic stability using the heat of formation. It was found that B2 Mn50Pt50−xPdx alloys (0 ≤ x ≤ 18.75) are thermodynamically stable due to the negative heat of formation close to that of a pristine MnPt alloy. Based on the elasticity results, the B2 Mn50Pt50−xPdx is most likely to undergo martensitic transformation for the entire considered composition range. From the calculated values of the Poisson′s ratio, it is shown that an increase in Pd, Ag, and Au effectively improves the ductility of the B2 Mn50Pt50−xMx compounds. It was revealed that ferromagnetism is maintained with Pd addition but significantly reduced in the case of Au and Ag. Thus, this work showed that density functional theory can be exploited to propose new possible compositions for future magnets in spintronic applications.
Microstructural Heterogeneity and Property Variations in Cast and Vacuum Hot-Pressed CoCrPtB Alloy
Authors: Li Yan , Guan Weiming , Wen Ming , Guo Junmei , Chen Ze , Wang Chuanjun
Published: January 2024

Limited research has been undertaken regarding the homogeneity of CoCrPtB alloy billets. A CoCrPtB alloy was processed through casting and vacuum hot pressing. This investigation delved into the interconnection between the secondary dendrite arm spacing (SDAS) in the as-hot-pressed samples and their corresponding attributes, specifically Vickers hardness and magnetic properties. Systematic sampling was conducted on the cross-sectional layer and longitudinal surface. Upon examination of the cross-sectional layer proximate to the uppermost region of the hot casting, a discernible parabolic trend was observed for the SDAS that exhibited a gradual increment from the peripheral regions toward the central area along the width. Simultaneously, the fraction of the dendrite phase displayed a consistent linear decline, attaining its peak value at the central portion of the billet. Conversely, on the longitudinal surface, SDAS and the fraction of the dendrite phase remained fairly uniform within the same column sampling regions. However, a notable divergence was identified in the central section, characterized by an augmented SDAS and diminished dendrite phase content. This inherent microstructural inhomogeneity within the CoCrPtB alloy engendered discernible disparities in material properties.
Three New Proofs of the Theorem rank f(M) + rank g(M) = rank (f,g)(M) + rank [f,g](M)
Authors: Pop Vasile , Negrescu Alexandru
Published: January 2024

It is well known that in C[X], the product of two polynomials is equal to the product of their greatest common divisor and their least common multiple. In a recent paper, we proved a similar relation between the ranks of matrix polynomials. More precisely, the sum of the ranks of two matrix polynomials is equal to the sum of the rank of the greatest common divisor of the polynomials applied to the respective matrix and the rank of the least common multiple of the polynomials applied to the respective matrix. In this paper, we present three new proofs for this result. In addition to these, we present two more applications.
Intuitionistic Fuzzy Modal Multi-Topological Structures and Intuitionistic Fuzzy Multi-Modal Multi-Topological Structures
Authors: Atanassov Krassimir
Published: January 2024

On the basis of K. Kuratowski’s definitions of a topological structure with a closure or interior operator, the concept of a modal topological structure (MTS) with one of these operators was introduced by the author. This new structure was illustrated with examples with intuitionistic fuzzy topological operators from both examples, and for this reason, these structures were named intuitionistic fuzzy MTSs (IFMTSs). In a series of papers, the author introduced some modifications and extensions to the IFMTSs, e.g., intuitionistic fuzzy temporal topological structures, intuitionistic fuzzy level topological structures and others, and intuitionistic fuzzy multi modal topological structures and others. In the present paper, four new examples of intuitionistic fuzzy multi modal topological structures are given. On their base, the concepts of a modal multi-topological structure and of a multi-modal multi-topological structure are introduced and illustrated with examples from the area of the intuitionistic fuzzy sets—intuitionistic fuzzy modal multi-topological structure with a closure or an interior operator; and intuitionistic fuzzy multi-modal multi-topological structure with one of these operators. Two intuitionistic fuzzy topological operators are defined. Their basic properties are studied and they are used in the new structures.
Understanding Melt Pool Behavior of 316L Stainless Steel in Laser Powder Bed Fusion Additive Manufacturing
Authors: Zhang Zilong , Zhang Tianyu , Sun Can , Karna Sivaji , Yuan Lang
Published: January 2024

In the laser powder bed fusion additive manufacturing process, the quality of fabrications is intricately tied to the laser–matter interaction, specifically the formation of the melt pool. This study experimentally examined the intricacies of melt pool characteristics and surface topography across diverse laser powers and speeds via single-track laser scanning on a bare plate and powder bed for 316L stainless steel. The results reveal that the presence of a powder layer amplifies melt pool instability and worsens irregularities due to increased laser absorption and the introduction of uneven mass from the powder. To provide a comprehensive understanding of melt pool dynamics, a high-fidelity computational model encompassing fluid dynamics, heat transfer, vaporization, and solidification was developed. It was validated against the measured melt pool dimensions and morphology, effectively predicting conduction and keyholing modes with irregular surface features. Particularly, the model explained the forming mechanisms of a defective morphology, termed swell-undercut, at high power and speed conditions, detailing the roles of recoil pressure and liquid refilling. As an application, multiple-track simulations replicate the surface features on cubic samples under two distinct process conditions, showcasing the potential of the laser–matter interaction model for process optimization.
Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans
Authors: Simin Stanislav , Tomanović Snežana , Sukara Ratko , Stefanov Marijana , Savović Milan , Gajić Bojan , Lalošević Vesna
Published: January 2024

The “beautiful viviparous fly”, Wohlfahrtia magnifica, may have a magnificent appearance due to its striking morphology; however, it is a potentially deadly agent of obligate traumatic myiasis in humans and animals, with a serious impact on welfare and economics. The fly is found across the Palearctic realm, including the Western Balkan region, with reports from former Yugoslavian countries from the first half of the 20th century. In this paper, a recent case of wohlfahrtiosis recorded in Northern Serbia is evidenced using morphological and molecular techniques. Larvae were collected from two adult sheep with severe hoof myiasis and two young sheep with genital and interdigital myiasis. Morphological identification was performed for adults bred from the infested vulva and third-stage larvae (L3) collected from the hoof wounds, supported with barcoding sequences of the COI gene obtained from larval pairs from the hoof wounds of older and the genitalia of younger sheep. W. magnifica was identified according to the appearance of male fly terminalia and the morphology of L3, which was confirmed after the comparison of representative sequences of the COI gene (deposited in GenBank™ under accession numbers MT027108–MT027114) to those available in GenBank™. This finding represents the first reported case of wohlfahrtiosis in the Western Balkans in 80 years, highlighting the need to re-inform relevant stakeholders to achieve adequate disease control.
The First Description of the Microbial Diversity in the Amarillo River (La Rioja, Argentina), a Natural Extreme Environment Where the Whole Microbial Community Paints the Landscape Yellow
Authors: Bernardelli Cecilia Elena , Colman Deborah , Donati Edgardo Ruben , Urbieta María Sofía
Published: January 2024

The Amarillo River in Famatina, La Rioja, Argentina, is a natural acidic river with distinctive yellow-ochreous iron precipitates along its course. While mining activities have occurred in the area, the river’s natural acidity is influenced by environmental factors beyond mineralogy, where microbial species have a crucial role. Although iron-oxidising bacteria have been identified, a comprehensive analysis of the entire microbial community in this extreme environment has not yet been conducted. In this study, we employ high-throughput sequencing to explore the bacterial and fungal diversity in the Amarillo River and Cueva de Pérez terraces, considered prehistoric analogues of the current river basin. Fe(II)-enrichment cultures mimicking different environmental conditions of the river were also analysed to better understand the roles of prokaryotes and fungi in iron oxidation processes. Additionally, we investigate the ecological relationships between bacteria and fungi using co-occurrence and network analysis. Our findings reveal a diverse bacterial community in the river and terraces, including uncultured species affiliated with Acidimicrobiia, part of an uncharacterised universal microbial acidic diversity. Acidophiles such as Acidithiobacillus ferrivorans, the main iron oxidiser of the system, and Acidiphilium, which is unable to catalyse Fe(II) oxidation but has a great metabolic flexibility,, are part of the core of the microbial community, showing significant involvement in intraspecies interactions. Alicyclobacillus, which is the main Fe(II) oxidiser in the enrichment culture at 30 °C and is detected all over the system, highlights its flexibility towards the iron cycle. The prevalence of key microorganisms in both rivers and terraces implies their enduring contribution to the iron cycle as well as in shaping the iconic yellow landscape of the Amarillo River. In conclusion, this study enhances our understanding of microbial involvement in iron mineral precipitation, emphasising the collaborative efforts of bacteria and fungi as fundamental geological agents in the Amarillo River.
Use of Trichoderma in the Production of Forest Seedlings
Authors: Ferreira Natália Cássia de Faria , Ramos Maria Lucrecia Gerosa , Gatto Alcides
Published: January 2024

Forest production has great relevance in the Brazilian economy, characterized by several production sectors, including the production of seedlings. With the focus on maximizing the capacity of survival, development, and adaptation of seedlings, Trichoderma is highlighted as a potentially useful genus of microorganisms for promoting growth and higher product quality. In this sense, this review aims to describe the main mechanisms of fungi action in forest seedlings’ production. The different species of the genus Trichoderma have specific mechanisms of action, and the current scenario points to more advances in the number of species. The interaction process mediated by different mechanisms of action begins in the communication with plants, from the colonization process. After the interaction, chemical dialogues allow the plant to develop better because, from colonization, the forest seedlings can maximize height and increase shoot and root development. Fungi promote solubilization and availability of nutrients to seedlings, which show numerous benefits to the development. The use of beneficial microorganisms, such as fungi of the genus Trichoderma, has become a sustainable strategy to enhance seedling development, reducing the use of agrochemicals and industrial fertilizers.
Application of Potentiometric and Electrophoretic Measurements to Evaluate the Reversibility of Adsorption of Divalent Ions from a Solution on Titanium Dioxide
Authors: Piasecki Wojciech , Lament Karolina
Published: January 2024

The adsorption of divalent ions on metal oxides is controlled by the pH of a solution. It is commonly assumed that this is a reversible process for pH changes. However, there are reports that the sorption of ions on oxides may not be reversible. To verify this, we used potentiometric titration, ion-selective electrodes (ISEs), and electrokinetic measurements to examine the reversibility of the adsorption of hydrogen ions and three metal ions (Ca2+, Cu2+, and Fe2+) on TiO2. The ferrous ion was used as a reference because its adsorption is entirely irreversible. The surface charge determined by potentiometric titration and the adsorption edges measured using ISE indicate that the adsorption of copper ions is reversible with changes in pH. In the case of calcium ions, the results suggest a certain degree of irreversibility. There are apparent differences in the electrokinetic potential data obtained during titration with base and acid, which suggests that the adsorption is irreversible. We have explained this contradiction by considering the complex and dynamic nature of electrophoretic mobility. In our opinion, potentiometric titration may be the simplest and most reliable method for assessing the reversibility of multivalent ion adsorption.
Self-Assembled Monolayers of Push–Pull Chromophores as Active Layers and Their Applications
Authors: Wang Junlong , Gadenne Virginie , Patrone Lionel , Raimundo Jean-Manuel
Published: January 2024

In recent decades, considerable attention has been focused on the design and development of surfaces with defined or tunable properties for a wide range of applications and fields. To this end, self-assembled monolayers (SAMs) of organic compounds offer a unique and straightforward route of modifying and engineering the surface properties of any substrate. Thus, alkane-based self-assembled monolayers constitute one of the most extensively studied organic thin-film nanomaterials, which have found wide applications in antifouling surfaces, the control of wettability or cell adhesion, sensors, optical devices, corrosion protection, and organic electronics, among many other applications, some of which have led to their technological transfer to industry. Nevertheless, recently, aromatic-based SAMs have gained importance as functional components, particularly in molecular electronics, bioelectronics, sensors, etc., due to their intrinsic electrical conductivity and optical properties, opening up new perspectives in these fields. However, some key issues affecting device performance still need to be resolved to ensure their full use and access to novel functionalities such as memory, sensors, or active layers in optoelectronic devices. In this context, we will present herein recent advances in π-conjugated systems-based self-assembled monolayers (e.g., push–pull chromophores) as active layers and their applications.
Processing Speed and Attentional Shift/Mental Flexibility in Patients with Stroke: A Comprehensive Review on the Trail Making Test in Stroke Studies
Authors: Tsiakiri Anna , Christidi Foteini , Tsiptsios Dimitrios , Vlotinou Pinelopi , Kitmeridou Sofia , Bebeletsi Paschalina , Kokkotis Christos , Serdari Aspasia , Tsamakis Konstantinos , Aggelousis Nikolaos , Vadikolias Konstantinos
Published: January 2024

The Trail Making Test (TMT) is one of the most commonly administered tests in clinical and research neuropsychological settings. The two parts of the test (part A (TMT-A) and part B (TMT-B)) enable the evaluation of visuoperceptual tracking and processing speed (TMT-A), as well as divided attention, set-shifting and cognitive flexibility (TMT-B). The main cognitive processes that are assessed using TMT, i.e., processing speed, divided attention, and cognitive flexibility, are often affected in patients with stroke. Considering the wide use of TMT in research and clinical settings since its introduction in neuropsychological practice, the purpose of our review was to provide a comprehensive overview of the use of TMT in stroke patients. We present the most representative studies assessing processing speed and attentional shift/mental flexibility in stroke settings using TMT and applying scoring methods relying on conventional TMT scores (e.g., time-to-complete part A and part B), as well as derived measures (e.g., TMT-(B-A) difference score, TMT-(B/A) ratio score, errors in part A and part B). We summarize the cognitive processes commonly associated with TMT performance in stroke patients (e.g., executive functions), lesion characteristics and neuroanatomical underpinning of TMT performance post-stroke, the association between TMT performance and patients’ instrumental activities of daily living, motor difficulties, speech difficulties, and mood statue, as well as their driving ability. We also highlight how TMT can serve as an objective marker of post-stroke cognitive recovery following the implementation of interventions. Our comprehensive review underscores that the TMT stands as an invaluable asset in the stroke assessment toolkit, contributing nuanced insights into diverse cognitive, functional, and emotional dimensions. As research progresses, continued exploration of the TMT potential across these domains is encouraged, fostering a deeper comprehension of post-stroke dynamics and enhancing patient-centered care across hospitals, rehabilitation centers, research institutions, and community health settings. Its integration into both research and clinical practice reaffirms TMT status as an indispensable instrument in stroke-related evaluations, enabling holistic insights that extend beyond traditional neurological assessments.
Diet-Induced Severe Hyperhomocysteinemia Promotes Atherosclerosis Progression and Dysregulates the Plasma Metabolome in Apolipoprotein-E-Deficient Mice
Authors: Andrews Stephen G. , Koehle Anthony M. , Paudel Devendra , Neuberger Thomas , Ross A. Catharine , Singh Vishal , Bottiglieri Teodoro , Castro Rita
Published: January 2024

Atherosclerosis and resulting cardiovascular disease are the leading causes of death in the US. Hyperhomocysteinemia (HHcy), or the accumulation of the intermediate amino acid homocysteine, is an independent risk factor for atherosclerosis, but the intricate biological processes mediating this effect remain elusive. Several factors regulate homocysteine levels, including the activity of several enzymes and adequate levels of their coenzymes, including pyridoxal phosphate (vitamin B6), folate (vitamin B9), and methylcobalamin (vitamin B12). To better understand the biological influence of HHcy on the development and progression of atherosclerosis, apolipoprotein-E-deficient (apoE−/− mice), a model for human atherosclerosis, were fed a hyperhomocysteinemic diet (low in methyl donors and B vitamins) (HHD) or a control diet (CD). After eight weeks, the plasma, aorta, and liver were collected to quantify methylation metabolites, while plasma was also used for a broad targeted metabolomic analysis. Aortic plaque burden in the brachiocephalic artery (BCA) was quantified via 14T magnetic resonance imaging (MRI). A severe accumulation of plasma and hepatic homocysteine and an increased BCA plaque burden were observed, thus confirming the atherogenic effect of the HHD. Moreover, a decreased methylation capacity in the plasma and aorta, indirectly assessed by the ratio of S-adenosylmethionine to S-adenosylhomocysteine (SAM:SAH) was detected in HHD mice together with a 172-fold increase in aortic cystathionine levels, indicating increased flux through the transsulfuration pathway. Betaine and its metabolic precursor, choline, were significantly decreased in the livers of HHD mice versus CD mice. Widespread changes in the plasma metabolome of HHD mice versus CD animals were detected, including alterations in acylcarnitines, amino acids, bile acids, ceramides, sphingomyelins, triacylglycerol levels, and several indicators of dysfunctional lipid metabolism. This study confirms the relevance of severe HHcy in the progression of vascular plaque and suggests novel metabolic pathways implicated in the pathophysiology of atherosclerosis.
Sampling Procedures for Estimating the Infant Intake of Human Milk Leptin, Adiponectin, Insulin, Glucose, and Total Lipid
Authors: Suwaydi Majed A. , Lai Ching Tat , Gridneva Zoya , Perrella Sharon L. , Wlodek Mary E. , Geddes Donna T.
Published: January 2024

Limited attention is given to the efficacy of protocols for the estimation of infant intake of milk components when investigating their impact on infant outcomes. We compared the actual measured intake of human milk components with estimations derived from 15 protocols to determine the most reliable approach for estimating intake of HM leptin, adiponectin, insulin, glucose, and total lipid. Twenty mothers who were 3–5 months postpartum completed a 24 h milk profile study with pre-/post-feed milk samples collection. The true infant intake (control group) based on 24 h milk intake (MI) was compared to estimated infant intakes using concentrations from five sampling protocols that were multiplied by one of true infant MI, considered mean MI (800 mL), or global mean MI (766 mL). The mean measured concentrations of six samples (three sets of pre- and post-feed samples, from morning (06:00–09:00), afternoon (13:00–16:00), and evening (19:00–22:00)) multiplied by the true infant MI, mean considered MI, and global mean MI produced the most accurate estimates of infant intake of these components. Therefore, in the absence of 24 h measurements and sampling, a sampling protocol comprising three sets of pre-/post-feed samples provides the most reliable infant intake estimates of HM leptin, adiponectin, insulin, glucose, and total lipid.
Estimated Intake of Potassium, Phosphorus and Zinc with the Daily Diet Negatively Correlates with ADP-Dependent Whole Blood Platelet Aggregation in Older Subjects
Authors: Karolczak Kamil , Guligowska Agnieszka , Sołtysik Bartłomiej K. , Kostanek Joanna , Kostka Tomasz , Watala Cezary
Published: January 2024

The aggregation of blood platelets is the pivotal step that leads to thrombosis. The risk of thrombotic events increases with age. Available data suggest that minerals taken with diet can affect the course of thrombosis. However, little is known about the relationship between platelet aggregability and mineral intake with diet among elderly people. Thus, we evaluated the associations between the reactivities of platelets to arachidonic acid, collagen or ADP and the estimated quantities of minerals consumed as a part of the daily diet in 246 subjects aged 60–65 years (124 men and 122 women). The found simple (not-adjusted) Spearman’s rank negative correlations are as follows: 1. arachidonate-dependent aggregation and the amounts of potassium, zinc, magnesium, phosphorus, iron, copper and manganese; 2. collagen-dependent aggregation and the amounts of potassium, phosphorus, iron and zinc; and 3. ADP-dependent aggregation and the amounts of potassium, phosphorus and zinc. The negative associations between ADP-dependent platelet reactivity and the amount of potassium, phosphorus and zinc and between collagen-dependent aggregability and the amount of phosphorus were also noted after adjusting for a bunch of cardiovascular risk factors. Overall, in older subjects, the intake of minerals with diet is negatively related to blood platelet reactivity, especially in response to ADP. Diet fortification with some minerals may possibly reduce the thrombotic risk among elderly patients.
Multivalent Epigraph Hemagglutinin Vaccine Protects against Influenza B Virus in Mice
Authors: Petro-Turnquist Erika , Corder Kampfe Brigette , Gadeken Amber , Pekarek Matthew J. , Weaver Eric A.
Published: January 2024

Influenza B virus is a respiratory pathogen that contributes to seasonal epidemics, accounts for approximately 25% of global influenza infections, and can induce severe disease in young children. While vaccination is the most commonly used method of preventing influenza infections, current vaccines only induce strain-specific responses and have suboptimal efficacy when mismatched from circulating strains. Further, two influenza B virus lineages have been described, B/Yamagata-like and B/Victoria-like, and the limited cross-reactivity between the two lineages provides an additional barrier in developing a universal influenza B virus vaccine. Here, we report a novel multivalent vaccine using computationally designed Epigraph hemagglutinin proteins targeting both the B/Yamagata-like and B/Victoria-like lineages. When compared to the quadrivalent commercial vaccine, the Epigraph vaccine demonstrated increased breadth of neutralizing antibody and T cell responses. After lethal heterologous influenza B virus challenge, mice immunized with the Epigraph vaccine were completely protected against both weight loss and mortality. The superior cross-reactive immunity conferred by the Epigraph vaccine immunogens supports their continued investigation as a universal influenza B virus vaccine.
Netazepide, an Antagonist of Cholecystokinin Type 2 Receptor, Prevents Vincristine-Induced Sensory Neuropathy in Mice
Authors: Bernard Amandine , Mroué Mohamad , Bourthoumieu Sylvie , Boyce Malcolm , Richard Laurence , Sturtz Franck , Demiot Claire , Danigo Aurore
Published: January 2024

Among the vinca-alkaloid class, vincristine is a potent chemotherapeutic agent with significant neurotoxic effects and is employed to address a wide spectrum of cancer types. Recently, the therapeutic potential of the cholecystokinin type 2 receptor (CCK2R) as a target for vincristine-induced peripheral neuropathy (VIPN) was demonstrated. In this study, the impact of preventive CCK2R blockade using netazepide (Trio Medicines Ltd., London, UK) was investigated in a mouse model of vincristine-induced peripheral neuropathy. Netazepide is a highly selective CCK2R antagonist under development for the treatment of patients with gastric neuroendocrine tumors caused by hypergastrinemia secondary to chronic autoimmune atrophic gastritis. Vincristine-induced peripheral neuropathy was induced by intraperitoneal injections of vincristine at 100 µg/kg/d for 7 days (D0 to D7). Netazepide (2 mg/kg/d or 5 mg/kg/d, per os) was administered one day before vincristine treatment until D7. Vincristine induced a high tactile allodynia from D1 to D7. VIPN was characterized by dorsal root ganglion neuron (DRG) and intraepidermal nerve fiber (IENF) loss, and enlargement and loss of myelinated axons in the sciatic nerve. Netazepide completely prevented the painful symptoms and nerve injuries induced by vincristine. In conclusion, the fact that netazepide protected against vincristine-induced peripheral neuropathy in a mouse model strongly supports the assessment of its therapeutic potential in patients receiving such chemotherapy.
Effects of Tenofovir Disoproxil Fumarate on Bone Quality beyond Bone Density—A Scoping Review of the Literature
Authors: Hashwin Singh Tejpal Singh , Jashwin Singh Tejpal Singh , Chin Kok-Yong
Published: January 2024

Tenofovir disoproxil fumarate (TDF) is a widely used pharmacological agent for the treatment of human immunodeficiency virus infection. While prolonged exposure to TDF has been associated with a decrease in bone mineral density (BMD) and increased fracture risk, limited discussion exists on its effects on various aspects of bone quality. This scoping review aims to provide a comprehensive overview of the impact of TDF on bone quality beyond BMD. A literature search was conducted using the PubMed and Scopus databases to identify studies investigating the effects of TDF on bone quality. Original research articles written in English, irrespective of study type or publication year, were included in the review. Seven articles met the inclusion criteria. Findings indicate that prolonged exposure to TDF adversely affects bone microarchitecture and strength, impeding fracture healing and skeletal microdamage repair. The observed effects suggest a complex interplay involving bone cell signalling, cytokines and bone remodelling processes as potential mechanisms underlying TDF’s impact on bone quality. As a conclusion, TDF impairs bone remodelling and microarchitecture by influencing dynamic bone cell behaviour and signalling pathways. Future studies should delve deeper into understanding the intricate negative effects of TDF on bone and explore strategies for reversing these effects.
Accelerated Neuroimmune Dysfunction in Aged HIV-1-Infected Humanized Mice
Authors: Zhang Chen , Su Hang , Waight Emiko , Poluektova Larisa Y. , Gorantla Santhi , Gendelman Howard E. , Dash Prasanta K.
Published: January 2024

Disordered immunity, aging, human immunodeficiency virus type one (HIV-1) infection, and responses to antiretroviral therapy are linked. However, how each factor is linked with the other(s) remains incompletely understood. It has been reported that accelerated aging, advanced HIV-1 infection, inflammation, and host genetic factors are associated with host cellular, mitochondrial, and metabolic alterations. However, the underlying mechanism remains elusive. With these questions in mind, we used chronically HIV-1-infected CD34-NSG humanized mice (hu-mice) to model older people living with HIV and uncover associations between HIV-1 infection and aging. Adult humanized mice were infected with HIV-1 at the age of 20 weeks and maintained for another 40 weeks before sacrifice. Animal brains were collected and subjected to transcriptomics, qPCR, and immunofluorescence assays to uncover immune disease-based biomarkers. CD4+ T cell decline was associated with viral level and age. Upregulated C1QA, CD163, and CXCL16 and downregulated LMNA and CLU were identified as age-associated genes tied to HIV-1 infection. Ingenuity pathway analysis affirmed links to innate immune activation, pyroptosis signaling, neuroinflammation, mitochondrial dysfunction, cellular senescence, and neuronal dysfunction. In summary, CD34-NSG humanized mice are identified as a valuable model for studying HIV-1-associated aging. Biomarkers of immune senescence and neuronal signaling are both age- and virus-associated. By exploring the underlying biological mechanisms that are linked to these biomarkers, interventions for next generation HIV-1-infected patients can be realized.
RETRACTED: Alhakamy et al. Fluoxetine Ecofriendly Nanoemulsion Enhances Wound Healing in Diabetic Rats: In Vivo Efficacy Assessment. Pharmaceutics 2022, 14, 1133
Authors: Alhakamy Nabil A. , Caruso Giuseppe , Privitera Anna , Ahmed Osama A. A. , Fahmy Usama A. , Md Shadab , Mohamed Gamal A. , Ibrahim Sabrin R. M. , Eid Basma G. , Abdel-Naim Ashraf B. , Caraci Filippo
Published: January 2024

The journal retracts the article, “Fluoxetine Ecofriendly Nanoemulsion Enhances Wound Healing in Diabetic Rats: In Vivo Efficacy Assessment” [...]
A Catalog of GNI-A1 Genes That Regulate Floret Fertility in a Diverse Bread Wheat Collection
Authors: Sakuma Shun , Yamashita Yoko , Suzuki Takako , Nasuda Shuhei
Published: January 2024

Modifying inflorescence architecture improves grain number and grain weight in bread wheat (Triticum aestivum). Allelic variation in Grain Number Increase 1 (GNI-A1) genes, encoding a homeodomain leucine zipper class I transcription factor, influences grain number and yield. However, allelic information about GNI-A1 in diverse germplasms remains limited. Here, we investigated GNI-A1 alleles in a panel of 252 diverse bread wheat accessions (NBRP core collection and HRO breeder’s panel) by target resequencing. Cultivars carrying the reduced-function allele (105Y) were predominant in the NBRP panel, whereas the 105N functional allele was the major type in the HRO panel. Cultivars with the 105Y allele were distributed in Asian landraces but not in European genotypes. Association analysis demonstrated that floret fertility, together with grain size, were improved in cultivars in the NBRP core collection carrying the 105Y allele. These results imply that different alleles of GNI-A1 have been locally selected, with the 105Y allele selected in East Asia and the 105N allele selected in Europe.
Phytochemical Composition and Biological Activity
Authors: Fais Antonella , Era Benedetta
Published: January 2024

Phytochemicals are bioactive plant compounds that provide humans with health benefits, representing a valuable source of novel bioactive molecules [...]
Meta-Analysis of the Effects of Overexpressed bZIP Transcription Factors in Plants under Drought Stress
Authors: Tao Ran , Liu Yaqiu , Chen Su , Shityakov Sergey
Published: January 2024

The bZIP (basic leucine zipper) transcription factors have been identified as key regulators of plant responses to drought stress, which limits plant growth and yield. Overexpression of bZIP genes has shown potential in enhancing drought tolerance in various plant species. However, the constrained types of individual studies and inconsistencies among experimental approaches has resulted in a lack of statistical significance and limited the extrapolation of bZIP transcription factor overexpression for plant improvement. We conducted a meta-analysis to evaluate ten measured parameters of drought tolerance in bZIP transcription factor-expressing plants as well as moderators affecting the performance of transgenic plants. The results showed that seven parameters, including survival rate as well as the content of regulatory substances (proline accumulation, H2O2 concentration, CAT activity, POD activity, SOD activity and MDA accumulation), were most affected while the impact on physiological status indicators is not significant. In addition, donor/recipient species, treatment medium, duration and methods of simulating drought stress all significantly impacted the degree of drought stress tolerance in plants to some extent among the considered moderators. The findings underscore the potential of bZIP transcription factors as key targets for genetic engineering approaches aimed at improving plant resilience to water scarcity.
Preparation of High Specific Surface Area Activated Carbon from Petroleum Coke by KOH Activation in a Rotary Kiln
Authors: Wang Kechao , Xu Shaoping
Published: January 2024

In the preparation of high specific surface area activated carbon (AC) by KOH activation, the swelling of the reactant mixture and the particles’ agglomeration deteriorates the process and the property of product. In this study, a novel method using a rotary kiln loaded with steel balls has been developed for the preparation of AC from petroleum coke (PC) by KOH activation. It has been found that the molten KOH caused the swelling of the reaction mixture at a lower activation temperature, while the molten K2O led to the particles’ agglomeration at a higher temperature. The steel balls could relieve the swelling and agglomeration and enhance the pore structure development of the AC by boosting the heat and mass transfer in the reactor. At an activation temperature of 800 °C and a KOH/PC mass ratio of 3:1, the specific surface area of the AC obtained without the addition of steel balls in the kiln is 1492 m2/g, while that with the steel balls is 1996 m2/g. The introduction of CO2 during the activation could further decrease the particles’ agglomeration by converting the K2O into thermoset K2CO3 and develop more mesopores of AC. Specifically, the average pore size of the AC increased from 2.20 to 2.72 nm.
Risk Management in Islamic Banking: The Impact of Financial Technologies through Empirical Insights from the UAE
Authors: Al Hammadi Mohamed , Jimber-Del Río Juan Antonio , Ochoa-Rico María Salomé , Montero Orlando Arencibia , Vergara-Romero Arnaldo
Published: January 2024

Financial technology (fintech) innovations are transforming banking globally. Their adoption poses new opportunities and risks for Islamic banks with unique requirements. This study examines fintech’s implications for risk management effectiveness in United Arab Emirates Islamic banks. A conceptual model incorporates factors like fintech adoption, emerging capabilities, digital maturity, and IT security influencing outcomes. Primary data were collected via survey from nine UAE Islamic banks and analyzed using PLS-SEM. Results show that fintech adoption and capabilities positively impacted effectiveness, while digital transformation alone did not. The findings also show that the regulatory environment did not moderate relationships as hypothesized. The findings provide empirical evidence on optimizing risk management through responsible fintech enablement and oversight alignment in the UAE context.
ERS-HDRI: Event-Based Remote Sensing HDR Imaging
Authors: Li Xiaopeng , Cheng Shuaibo , Zeng Zhaoyuan , Zhao Chen , Fan Cien
Published: January 2024

High dynamic range imaging (HDRI) is an essential task in remote sensing, enhancing low dynamic range (LDR) remote sensing images and benefiting downstream tasks, such as object detection and image segmentation. However, conventional frame-based HDRI methods may encounter challenges in real-world scenarios due to the limited information inherent in a single image captured by conventional cameras. In this paper, an event-based remote sensing HDR imaging framework is proposed to address this problem, denoted as ERS-HDRI, which reconstructs the remote sensing HDR image from a single-exposure LDR image and its concurrent event streams. The proposed ERS-HDRI leverages a coarse-to-fine framework, incorporating the event-based dynamic range enhancement (E-DRE) network and the gradient-enhanced HDR reconstruction (G-HDRR) network. Specifically, to efficiently achieve dynamic range fusion from different domains, the E-DRE network is designed to extract the dynamic range features from LDR frames and events and perform intra- and cross-attention operations to adaptively fuse multi-modal data. A denoise network and a dense feature fusion network are then employed for the generation of the coarse, clean HDR image. Then, the G-HDRR network, with its gradient enhancement module and multiscale fusion module, performs structure enforcement on the coarse HDR image and generates a fine informative HDR image. In addition, this work introduces a specialized hybrid imaging system and a novel, real-world event-based remote sensing HDRI dataset that contains aligned remote sensing LDR images, remote sensing HDR images, and concurrent event streams for evaluation. Comprehensive experiments have demonstrated the effectiveness of the proposed method. Specifically, it improves state-of-the-art PSNR by about 30% and the SSIM score by about 9% on the real-world dataset.
Assessment of the Performance of Ultrasonography for Detecting Myofascial Trigger Points
Authors: Chen Han-Yu , Hong Chang-Zern , Hsieh Yueh-Ling
Published: January 2024

Needle electromyogram (EMG) research has suggested that endplate noise (EPN) is a characteristic of myofascial trigger points (MTrPs). Although several studies have observed MTrPs through ultrasonography, whether they are hyperechoic or hypoechoic in ultrasound images is still controversial. Therefore, this study determined the echogenicity of MTrP ultrasonography. In stage 1, the MTrP of rat masseter muscle was identified through palpation and marked. Needle EMG was performed to detect the presence of EPN. When EPN was detected, ultrasound scans and indwelling needles were used to identify the nodule with a different grayscale relative to that of its surrounding tissue, and the echogenicity of the identified MTrP was determined. In stage 2, these steps were reversed. An ultrasound scan was performed to detect the nodule at the marked site, and an EMG needle was inserted into the nodule to detect EPN. There were 178 recordings in each stage, obtained from 45 rats. The stage 1 results indicate that the MTrPs in ultrasound images were hypoechoic with a 100% sensitivity of assessment. In stage 2, the accuracy and precision of MTrP detection through ultrasonography were 89.9% and 89.2%, respectively. The results indicate that ultrasonography produces highly accurate and precise MTrP detection results.