bluuebunny/crossref_metadata_2025 huggingface dataset:
Antimalarial and antitumour activities of the steroidal quinone-methide celastrol and its combinations with artemiside, artemisone and methylene blue
Authors: Ng Jerome P. L. , Han Yu , Yang Li Jun , Birkholtz Lyn-Marie , Coertzen Dina , Wong Ho Ning , Haynes Richard K. , Coghi Paolo , Wong Vincent Kam Wai
Published: September 2022

Artemisinin, isolated from the traditional Chinese medicinal plant qīng hāo 青蒿 (Artemisia annua) and its derivatives are used for treatment of malaria. With treatment failures now being recorded for the derivatives and companion drugs used in artemisinin combination therapies new drug combinations are urgently required. The amino-artemisinins artemiside and artemisone display optimal efficacies in vitro against asexual and sexual blood stages of the malaria parasite Plasmodium falciparum and are active against tumour cell lines. In continuing the evolution of combinations of the amino-artemisinins with new drugs, we examine the triterpenoid quinone methide celastrol isolated from the traditional Chinese medicinal plant léi gōng téng 雷公藤 (Tripterygium wilfordii). This compound is redox active, and has attracted considerable attention because of potent biological activities against manifold targets. We report that celastrol displays good IC50 activities ranging from 0.50–0.82 µM against drug-sensitive and resistant asexual blood stage Pf, and 1.16 and 0.28 µM respectively against immature and late stage Pf NF54 gametocytes. The combinations of celastrol with each of artemisone and methylene blue against asexual blood stage Pf are additive. Given that celastrol displays promising antitumour properties, we examined its activities alone and in combinations with amino-artemisinins against human liver HepG2 and other cell lines. IC50 values of the amino-artemisinins and celastrol against HepG2 cancer cells ranged from 0.55–0.94 µM. Whereas the amino-artemisinins displayed notable selectivities (SI > 171) with respect to normal human hepatocytes, in contrast, celastrol displayed no selectivity (SI < 1). The combinations of celastrol with artemiside or artemisone against HepG2 cells are synergistic. Given the promise of celastrol, judiciously designed formulations or structural modifications are recommended for mitigating its toxicity.
RAB6B is a potential prognostic marker and correlated with the remolding of tumor immune microenvironment in hepatocellular carcinoma
Authors: Peng Hao , Zhu Erwei , Wang Jitao , Du Xuanlong , Wang Chonggao , Yang Meng , Zhang Yewei
Published: September 2022

Backgrounds: Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer and the second leading cause of death among all cancers. The Ras-associated binding (Rab) proteins constitute the largest family of the Ras superfamily of small GTPases, which mainly mediate membrane trafficking processes. RAB6B is a member of Rab GTPases, and it has been found to be dysregulated in various tumors. However, the clinical significance, correlations with immune cells, and stroma infiltration of RAB6B in HCC remain unclear.

Methods: RAB6B mRNA and protein expression in HCC were examined using the TIMER, HCCDB, UALCAN, and HPA databases. The genetic alterations of RAB6B were analyzed by cBioPortal and COSMIC databases. The correlations between RAB6B and tumor-infiltrating immune cells and cancer-associated fibroblasts were explored by using TIMER, TISIDB, and GEPIA databases. Co-expression networks of RAB6B were investigated based on LinkedOmics. Drug sensitivity was analyzed through the GDSC and CTRP databases. RAB6B was knocked down with siRNA in HCC cell lines. EdU assay was performed to detect the cell proliferation ability, flow cytometry was used to compare the differences in the ability of apoptosis, and MTT was used to evaluate the drug sensitivity in vitro.

Results: RAB6B mRNA and protein expression were upregulated in the HCC tissues. Kaplan–Meier and Cox regression analyses suggested that highly expressed RAB6B was an independent prognostic factor for poor survival in HCC patients. Moreover, we found that RAB6B expression was positively correlated with the infiltration of immune cells in HCC, including some immunosuppressive cells, chemokines, and receptors, meanwhile RAB6B expression was associated with CD8+T cells exhaustion, resulting in an immunosuppressive microenvironment. Additionally, functional enrichment analysis indicated that RAB6B may be involved in ECM remodeling in the TME, and RAB6B expression was positively associated with CAFs infiltration. Furthermore, RAB6B presented a positive association with sensitivity to GDSC and CTRP drugs. RAB6B knockdown inhibited the cell proliferation and promoted apoptosis and sensitivity to cisplatin of HCC cells in vitro.

Conclusion: Our study revealed that RAB6B is a potential biomarker for poor prognosis in HCC patients and correlates with the formation of the immunosuppressive microenvironment in HCC.
Curcumin against gastrointestinal cancer: A review of the pharmacological mechanisms underlying its antitumor activity
Authors: Fan Yuanyuan , Zhang Xiqin , Tong Yuxin , Chen Suning , Liang Jingjing
Published: September 2022

Gastrointestinal cancer (GIC) poses a serious threat to human health globally. Curcumin (CUR), a hydrophobic polyphenol extracted from the rhizome of Curcuma longa, has shown reliable anticancer function and low toxicity, thereby offering broad research prospects. Numerous studies have demonstrated the pharmacological mechanisms underlying the effectiveness of CUR against GIC, including the induction of apoptosis and autophagy, arrest of the cell cycle, inhibition of the epithelial–mesenchymal transition (EMT) processes, inhibition of cell invasion and migration, regulation of multiple signaling pathways, sensitization to chemotherapy and reversal of resistance to such treatments, and regulation of the tumor survival environment. It has been confirmed that CUR exerts its antitumor effects on GIC through these mechanisms in vitro and in vivo. Moreover, treatment with CUR is safe and tolerable. Newly discovered types of regulated cell death (RCD), such as pyroptosis, necroptosis, and ferroptosis, may provide a new direction for research on the efficacy of CUR against GIC. In this review, we discuss the recently found pharmacological mechanisms underlying the effects of CUR against GIC (gastric and colorectal cancers). The objective is to provide a reference for further research on treatments against GIC.
32-Channel silicon strip detection module for combined X-ray fluorescence spectroscopy and X-ray diffractometry analysis
Authors: Carminati Marco , Borghi Giacomo , Demenev Evgeny , Gugiatti Matteo , Pepponi Giancarlo , Crivellari Michele , Ficorella Francesco , Ronchin Sabina , Zorzi Nicola , Borovin Evgeny , Lutterotti Luca , Fiorini Carlo
Published: September 2022

A compact detection module for the simultaneous measurement of XRF and XRD in portable analytical applications, in particular in the mining sector, is presented. The detector head is based on 32 silicon strip detectors, fabricated with a low-leakage technology by FBK and readout by two 16-channel low-noise CUBE charge-sensitive amplifiers. The design of the module and its characterization are reported. Multiple configurations are experimentally compared in terms of strip length, spacing, collimation and charge sharing effects. The optimal configuration for a strip length of 6 mm and pitch 0.2 mm is thus identified. It offers an energy resolution of better than 200 eV at 5.9 keV with moderate cooling (−10°C) and peaking time of 14 μs.
A method for identifying the important node in multi-layer logistic networks
Authors: Wang Chengwang , Xia Yongxiang , Zhu Lingzhe
Published: September 2022

Traditional methods to identify the important nodes are suitable for single networks. However, many real-world networks are coupled together, which can be modeled by multi-layer networks. Therefore, traditional identification methods may not be suitable for multi-layer networks. In this paper, we propose a new method to identify the important nodes in multi-layer logistic network. Considering the dynamic of the network, a new routing strategy based on the greedy algorithm and iterative method is proposed. The traditional betweenness centrality and closeness centrality are modified according to the new routing strategy to show the traffic condition and topology characteristics of each node. Then the new identification method is proposed based on the modified betweenness and closeness. The new method is compared with some traditional ones, and the simulation results show its advantages.
Involvement of transmembrane protein 184a during angiogenesis in zebrafish embryos
Authors: Field Cassandra J. , Perez Alexis M. , Samet Taryn , Ricles Victoria , Iovine M. Kathryn , Lowe-Krentz Linda J.
Published: September 2022

Angiogenesis, the outgrowth of new blood vessels from existing vasculature, is critical during development, tissue formation, and wound healing. In response to vascular endothelial growth factors (VEGFs), endothelial cells are activated to proliferate and move towards the signal, extending the vessel. These events are directed by VEGF-VEGF receptor (Vegfr2) signal transduction, which in turn is modulated by heparan sulfate proteoglycans (HSPGs). HSPGs are glycoproteins covalently attached to HS glycosaminoglycan chains. Transmembrane protein 184a (Tmem184a) has been recently identified as a heparin receptor, which is believed to bind heparan sulfate chains in vivo. Therefore, Tmem184a has the potential to fine-tune interactions between VEGF and HS, modulating Vegfr2-dependent angiogenesis. The function of Tmem184a has been investigated in the regenerating zebrafish caudal fin, but its role has yet to be evaluated during developmental angiogenesis. Here we provide insights into how Tmem184a contributes to the proper formation of the vasculature in zebrafish embryos. First, we find that knockdown of Tmem184a causes a reduction in the number of intact intersegmental vessels (ISVs) in the zebrafish embryo. This phenotype mimics that of vegfr2b knockout mutants, which have previously been shown to exhibit severe defects in ISV development. We then test the importance of HS interactions by removing the binding domain within the Tmem184a protein, which has a negative effect on angiogenesis. Tmem184a is found to act synergistically with Vegfr2b, indicating that the two gene products function in a common pathway to modulate angiogenesis. Moreover, we find that knockdown of Tmem184a leads to an increase in endothelial cell proliferation but a decrease in the amount of VE-cadherin present. Together, these findings suggest that Tmem184a is necessary for ISVs to organize into mature, complete vessels.
Effects of running fatigue on lower extremity symmetry among amateur runners: From a biomechanical perspective
Authors: Gao Zixiang , Fekete Gusztáv , Baker Julien S. , Liang Minjun , Xuan Rongrong , Gu Yaodong
Published: September 2022

The objective of this study was to examine the effects of running fatigue on the symmetry of lower limb biomechanical parameters in eighteen male amateur runners. The marker trajectories and ground reaction forces were collected before and after the running-induced fatigue protocol. Symmetry angles (SA) were used to quantify the symmetry of each parameter. Normality tests and Paired sample T-tests were carried out to detect bilateral lower limb differences and SA of parameters between pre- and post-fatigue. One-dimensional statistical parameter mapping (SPM_1d) was used to compare parameters with the characteristic of one-dimensional time series data of lower limbs. After fatigue, the SA of knee extension angles, knee abduction moment, and hip joint flexion moment increased by 17%, 10%, and 11% respectively. In comparison, the flexion angle of the knee joint decreased by 5%. The symmetry of internal rotation of ankle, knee and hip joints increased after fatigued, while the SA of external rotation of the three joints decreased significantly. These findings provide preliminary evidence that fatigue changes lower limb symmetry in running gait and may have implications for understanding running-related injuries and performance.
Exercise Induced-Cytokines Response in Marathon Runners: Role of ACE I/D and BDKRB2 +9/-9 Polymorphisms
Authors: Sierra Ana Paula Renno , Martínez Galán Bryan Steve , de Sousa Cesar Augustus Zocoler , de Menezes Duane Cardoso , Branquinho Jéssica Laís de Oliveira , Neves Raquel Leão , Arata Júlia Galanakis , Bittencourt Clarissa Azevedo , Barbeiro Hermes Vieira , de Souza Heraldo Possolo , Pesquero João Bosco , Cury-Boaventura Maria Fernanda
Published: September 2022

Renin-angiotensin system (RAS) and kallikrein-kinin system (KKS) have a different site of interaction and modulate vascular tone and inflammatory response as well on exercise adaptation, which is modulated by exercise-induced cytokines. The aim of the study was to evaluate the role of ACE I/D and BDKRB2 +9/−9 polymorphism on exercise-induced cytokine response. Seventy-four male marathon finishers, aged 30 to 55 years, participated in this study. Plasma levels of exercise-induced cytokines were determined 24 h before, immediately after, and 24 h and 72 h after the São Paulo International Marathon. Plasma concentrations of MCP-1, IL-6 and FGF-21 increased after marathon in all genotypes of BDKRB2. IL-10, FSTL and BDNF increased significantly after marathon in the genotypes with the presence of the −9 allele. FSTL and BDNF concentrations were higher in the −9/−9 genotype compared to the +9/+9 genotype before (p = 0.006) and after the race (p = 0.023), respectively. Apelin, IL-15, musclin and myostatin concentrations were significantly reduced after the race only in the presence of −9 allele. Marathon increased plasma concentrations of MCP1, IL-6, BDNF and FGF-21 in all genotypes of ACE I/D polymorphism. Plasma concentrations of IL-8 and MIP-1alpha before the race (p = 0.015 and p = 0.031, respectively), of MIP-1alpha and IL-10 after the race (p = 0.033 and p = 0.047, respectively) and VEGF 72 h after the race (p = 0.018) were lower in II homozygotes compared to runners with the presence of D allele. One day after the race we also observed lower levels of MIP-1alpha in runners with II homozygotes compared to DD homozygotes (p = 0.026). Before the marathon race myostatin concentrations were higher in DD compared to II genotypes (p = 0.009). Myostatin, musclin, IL-15, IL-6 and apelin levels decreased after race in genotypes with the presence of D allele. After the race ACE activity was negatively correlated with MCP1 (r = −56, p < 0.016) and positively correlated with IL-8, IL-10 and MIP1-alpha (r = 0.72, p < 0.0007, r = 0.72, p < 0.0007, r = 0.47, p < 0.048, respectively). The runners with the −9/−9 genotype have greater response in exercise-induced cytokines related to muscle repair and cardioprotection indicating that BDKRB2 participate on exercise adaptations and runners with DD genotype have greater inflammatory response as well as ACE activity was positively correlated with inflammatory mediators. DD homozygotes also had higher myostatin levels which modulates protein homeostasis.
Frequency-dependent signaling in cardiac myocytes
Authors: Haftbaradaran Esfahani Payam , Westergren Jan , Lindfors Lennart , Knöll Ralph
Published: September 2022

Background: Recent experimental data support the view that signaling activity at the membrane depends on its geometric parameters such as surface area and curvature. However, a mathematical, biophysical concept linking shape to receptor signaling is missing. The membranes of cardiomyocytes are constantly reshaped due to cycles of contraction and relaxation. According to constant-volume behavior of cardiomyocyte contraction, the length shortening is compensated by Z-disc myofilament lattice expansion and dynamic deformation of membrane between two adjacent Z-discs. Both morphological changes are strongly dependent on the frequency of contraction. Here, we developed the hypothesis that dynamic geometry of cardiomyocytes could be important for their plasticity and signaling. This effect may depend on the frequency of the beating heart and may represent a novel concept to explain how changes in frequency affect cardiac signaling.

Methods: This hypothesis is almost impossible to answer with experiments, as the in-vitro cardiomyocytes are almost two-dimensional and flattened rather than being in their real in-vivo shape. Therefore, we designed a COMSOL multiphysics program to mathematically model the dynamic geometry of a human cardiomyocyte and explore whether the beating frequency can modulate membrane signal transduction. Src kinase is an important component of cardiac mechanotransduction. We first presented that Src mainly localizes at costameres. Then, the frequency-dependent signaling effect was studied mathematically by numerical simulation of Src-mediated PDGFR signaling pathway. The reaction-convection-diffusion partial differential equation was formulated to simulate PDGFR pathway in a contracting sarcomeric disc for a range of frequencies from 1 to 4 Hz. Results: Simulations exhibits higher concentration of phospho-Src when a cardiomyocyte beats with higher rates. The calculated phospho-Src concentration at 4, 2, and 1 Hz beat rates, comparing to 0 Hz, was 21.5%, 9.4%, and 4.7% higher, respectively.

Conclusion: Here we provide mathematical evidence for a novel concept in biology. Cell shape directly translates into signaling, an effect of importance particularly for the myocardium, where cells continuously reshape their membranes. The concept of locality of surface-to-volume ratios is demonstrated to lead to changes in membrane-mediated signaling and may help to explain the remarkable plasticity of the myocardium in response to biomechanical stress.
Oxidative stress in Physella acuta: An integrative response of exposure to water from two rivers of Atlantic Mexican slope
Authors: Sedeño-Díaz Jacinto Elías , López-López Eugenia
Published: September 2022

Freshwater pollution is a complex mixture of xenobiotics due to the wastewater and the various chemicals routinely applied to agricultural lands that are discharged into water bodies. Xenobiotics can exert damage to the aquatic biota threatening the biodiversity of aquatic ecosystems. The oxidative damage and antioxidant responses have been widely investigated in freshwater organisms, mainly in fish and some invertebrates but in freshwater snails are scarce. This study aimed to assess the oxidative stress exerted by potential toxicity of water from two rivers of the Mexican Atlantic Slope (Tecolutla and Tuxpan rivers) in a freshwater mollusk Physella acuta. Lipid peroxidation level and a battery of antioxidant enzymes (Superoxide dismutase, Catalase and Glutathione peroxidase) were measured in P. acuta. The results are contextualized from an ecological point of view, associating the bioassay results with water quality characteristics. Water samples were obtained from three study sites for each river (in two seasons: Northern wind and dry). Twelve water quality variables were analyzed, and an additional water sample was used to perform a static bioassay for 96 h with snails grown in laboratory. After the exposure, we assessed lipid peroxidation level and the antioxidant responses of P. acuta exposed to water of rivers, and the Integrated Biomarker Response was computed. The highest lipid peroxidation level occurred in organisms exposed to water during the Northern wind season in both rivers. During this season, in the Tecolutla river, the superoxide dismutase activity was able to counteract the lipid peroxidation process, representing an adaptive response. In contrast, in the Tuxpan river, the superoxide dismutase was unable to counteract that process, stimulating CAT and GPx activities. The Integrated Biomarker Response showed that the Tecolutla river had higher values in the upper reaches than the Tuxpan river, showing a decreasing downstream gradient in both seasons. In the Tuxpan river, during the Dry season, the IBR score showed an increasing downstream gradient. During the Northern wind season, the IBR was higher in the upper reaches of both rivers, possibly due to the increased materials transported by runoff from the catchment, which includes a complex mixture of xenobiotics that affects the health of the sentinel species and aquatic biota in general. Based on our results, Physella acuta is proposed as sentinel species.
Macrophage migration inhibitory factor in acute kidneyinjury
Authors: Du Yiwei , Hao Hao , Ma Heng , Liu Hongbao
Published: September 2022

Acute kidney injury (AKI) is a complex clinical syndrome with multiple etiologies and pathogenesis, which lacks early biomarkers and targeted therapy. Recently, macrophage migration inhibitory factor (MIF) family protein have received increasing attention owing to its pleiotropic protein molecule character in acute kidney injury, where it performed a dual role in the pathological process. macrophage migration inhibitory factor and macrophage migration inhibitory factor-2 are released into the peripheral circulation when Acute kidney injury occurs and interact with various cellular pathways. On the one hand, macrophage migration inhibitory factor exerts a protective effect in anti-oxidation and macrophage migration inhibitory factor-2 promotes cell proliferation and ameliorates renal fibrosis. On the other hand, macrophage migration inhibitory factor aggravates renal injury as an upstream inflammation factor. Herein, we provide an overview on the biological role and possible mechanisms of macrophage migration inhibitory factor and macrophage migration inhibitory factor-2 in the process of Acute kidney injury and the clinical application prospects of macrophage migration inhibitory factor family proteins as a potential therapeutic target.
Abnormal ECG detection based on an adversarial autoencoder
Authors: Shan Lianfeng , Li Yu , Jiang Hua , Zhou Peng , Niu Jing , Liu Ran , Wei Yuanyuan , Peng Jiao , Yu Huizhen , Sha Xianzheng , Chang Shijie
Published: September 2022

Automatic detection and alarm of abnormal electrocardiogram (ECG) events play an important role in an ECG monitor system; however, popular classification models based on supervised learning fail to detect abnormal ECG effectively. Thus, we propose an ECG anomaly detection framework (ECG-AAE) based on an adversarial autoencoder and temporal convolutional network (TCN) which consists of three modules (autoencoder, discriminator, and outlier detector). The ECG-AAE framework is trained only with normal ECG data. Normal ECG signals could be mapped into latent feature space and then reconstructed as the original ECG signal back in our model, while abnormal ECG signals could not. Here, the TCN is employed to extract features of normal ECG data. Then, our model is evaluated on an MIT-BIH arrhythmia dataset and CMUH dataset, with an accuracy, precision, recall, F1-score, and AUC of 0.9673, 0.9854, 0.9486, 0.9666, and 0.9672 and of 0.9358, 0.9816, 0.8882, 0.9325, and 0.9358, respectively. The result indicates that the ECG-AAE can detect abnormal ECG efficiently, with its performance better than other popular outlier detection methods.
Physical exercise improved muscle strength and pain on neck and shoulder in military pilots
Authors: Heng Wei , Wei Feilong , Liu Zhisheng , Yan Xiaodong , Zhu Kailong , Yang Fan , Du Mingrui , Zhou Chengpei , Qian Jixian
Published: September 2022

Purpose: To evaluate the effects of physical exercise on neck and shoulder muscle strength and pain in military pilots.

Method: Embase, PubMed, and Cochrane Library databases were searched studies published up to April 1, 2022. Studies that met the screening criteria were included in the final meta-analysis. We calculated neck and shoulder maximal voluntary isometric contractions (MVICs), prevalence of pain, and pain intensity. Heterogeneity was explored by subgroup and sensitivity analyses.

Result: A total of 15 studies with 907 participants were included. In the exercise group, muscle strength was significantly increased in four directions of neck motion: flexion (standardized mean difference (SMD) = 0.45; 95% CI, 0.08–0.82), extension (SMD = 0.63; 95% CI, 0.27–1.00), right lateral flexion (Rtflx) (SMD = 0.53; 95% CI, 0.12–0.94), and left lateral flexion (Ltflx) (SMD = 0.50; 95% CI, 0.09–0.91). Subgroup analysis showed that fighter pilots, strength plus endurance training, and a follow-up period <20 weeks exhibited more significant muscle strength improvements than helicopter pilots, simple strength training, and a follow-up period ≥20 weeks. Overall, the pooled odds ratio (OR) for the effect of physical exercise on the prevalence of neck pain was not statistically significant (I2 = 60%). Sensitivity analysis revealed that the heterogeneity was restored after removing each of two studies (I2 = 47%), and the pooled OR was statistically significant (OR = 0.46; 95% CI, 0.23 to 0.94, or OR = 0.47; 95% CI, 0.24–0.91). Furthermore, compared with observational studies (OS), the reduction in the prevalence of neck pain was more significant in randomized controlled trials (RCTs) (OR = 0.37; 95% CI, 0.18–0.78). No significant differences in the effects of exercise on shoulder muscle strength and neck and shoulder pain intensity were observed.

Conclusion: Physical exercise can improve neck muscle strength in military pilots. After removing studies that may be the source of heterogeneity, exercise showed a protective effect on neck pain, especially in RCTs. The conclusion that exercise had no effects on shoulder muscle strength and pain intensity should be taken with caution.
Integrating a growth degree-days based reaction norm methodology and multi-trait modeling for genomic prediction in wheat
Authors: Raffo Miguel Angel , Sarup Pernille , Andersen Jeppe Reitan , Orabi Jihad , Jahoor Ahmed , Jensen Just
Published: September 2022

Multi-trait and multi-environment analyses can improve genomic prediction by exploiting between-trait correlations and genotype-by-environment interactions. In the context of reaction norm models, genotype-by-environment interactions can be described as functions of high-dimensional sets of markers and environmental covariates. However, comprehensive multi-trait reaction norm models accounting for marker × environmental covariates interactions are lacking. In this article, we propose to extend a reaction norm model incorporating genotype-by-environment interactions through (co)variance structures of markers and environmental covariates to a multi-trait reaction norm case. To do that, we propose a novel methodology for characterizing the environment at different growth stages based on growth degree-days (GDD). The proposed models were evaluated by variance components estimation and predictive performance for winter wheat grain yield and protein content in a set of 2,015 F6-lines. Cross-validation analyses were performed using leave-one-year-location-out (CV1) and leave-one-breeding-cycle-out (CV2) strategies. The modeling of genomic [SNPs] × environmental covariates interactions significantly improved predictive ability and reduced the variance inflation of predicted genetic values for grain yield and protein content in both cross-validation schemes. Trait-assisted genomic prediction was carried out for multi-trait models, and it significantly enhanced predictive ability and reduced variance inflation in all scenarios. The genotype by environment interaction modeling via genomic [SNPs] × environmental covariates interactions, combined with trait-assisted genomic prediction, boosted the benefits in predictive performance. The proposed multi-trait reaction norm methodology is a comprehensive approach that allows capitalizing on the benefits of multi-trait models accounting for between-trait correlations and reaction norm models exploiting high-dimensional genomic and environmental information.
Dissecting the genetic basis of bioactive metabolites and fruit quality traits in blueberries (Vaccinium corymbosum L.)
Authors: Mengist Molla Fentie , Grace Mary H. , Mackey Ted , Munoz Bryan , Pucker Boas , Bassil Nahla , Luby Claire , Ferruzzi Mario , Lila Mary Ann , Iorizzo Massimo
Published: September 2022

Blueberry is well-recognized as a healthy fruit with functionality derived largely from anthocyanin and chlorogenic acid. Despite their importance, no study to date has evaluated the genetic basis of these bioactives in blueberries and their relationship with fruit quality traits. Hence, to fill this gap, a mapping population including 196 F1 individuals was phenotyped for anthocyanin and chlorogenic acid concentration and fruit quality traits (titratable acidity, pH, and total soluble solids) over 3 years and data were used for QTL mapping and correlation analysis. Total soluble solids and chlorogenic acid were positively correlated with glycosylated anthocyanin and total anthocyanin, respectively, indicating that parallel selection for these traits is possible. Across all the traits, a total of 188 QTLs were identified on chromosomes 1, 2, 4, 8, 9, 11 and 12. Notably, four major regions with overlapping major-effect QTLs were identified on chromosomes 1, 2, 4 and 8, and were responsible for acylation and glycosylation of anthocyanins in a substrate and sugar donor specific manner. Through comparative transcriptome analysis, multiple candidate genes were identified for these QTLs, including glucosyltransferases and acyltransferases. Overall, the study provides the first insights into the genetic basis controlling anthocyanins accumulation and composition, chlorogenic acid and fruit quality traits, and establishes a framework to advance genetic studies and molecular breeding for anthocyanins in blueberry.
Exploring chemical diversity in Glycine max cultivars: A multivariate approach in the search for bioactive compounds against Spodoptera cosmioides
Authors: Aguiar Maria Clara Santana , Mueller de Freitas Marcelo , de Freitas Carlos Alessandro , Boiça Júnior Arlindo Leal , Carneiro Renato Lajarim , da Silva Maria Fátima das Graças Fernandes , Fernandes João Batista , Forim Moacir Rossi
Published: September 2022

Soybean crop is regulated by abiotic and biotic stresses with great potential in reducing grain yield and quality. The selection of resistant cultivars is a promising approach for mitigating these damages. We evaluated the chemical profile of Glycine max leaves from different cultivars in order to explore their defense mechanisms against Spodoptera cosmioides caterpillars. We optimized solid–liquid extraction techniques using ultrasound bath and static headspace extraction. Additionally, we developed an approach based on liquid and gas chromatography for analyzing the chemical profile of G. max cultivars. The principal component analysis allowed the classification of transgenic cultivars, which are classified as susceptible to S. cosmioides, from those obtained by genetic improvement and resistant to the insect. Differences were observed in the abundance of phenolic glycosides, lipids, aldehydes, and alcohols. More specifically, S. cosmioides resistant cultivars presented molecules related to the jasmonic and salicylic acid pathways. Such data can contribute to a molecular understanding of phenotypic diversity in soybean cultivars, from plant quality to resistance mechanisms and adaptation, to environmental stress and herbivory.
Mental wellbeing among urban young adults in a developing country: A Latent Profile Analysis
Authors: Nguyen Thao Thi Phuong , Nguyen Tham Thi , Dam Vu Trong Anh , Vu Thuc Thi Minh , Do Hoa Thi , Vu Giang Thu , Tran Anh Quynh , Latkin Carl A. , Hall Brian J. , Ho Roger C. M. , Ho Cyrus S. H.
Published: September 2022

Introduction

This study aimed to explore the mental wellbeing profiles and their related factors among urban young adults in Vietnam.

Methods

A cross-sectional study was conducted in Hanoi, which is the capital of Vietnam. There were 356 Vietnamese who completed the Mental Health Inventory-5 (MHI-5) questionnaire. The Latent Profile Analysis (LPA) was used to identify the subgroups of mental wellbeing through five items of the MHI-5 scale as the continuous variable. Multinomial logistic regression was used to determine factors related to subgroups.

Results

Three classes represented three levels of MHI-5 score, which included “Poor mental health,” “Fair mental health,” and “Good mental health,” were, respectively, 14.3, 46.6, and 39.0%. Compared to a low household economy, participants with an average household economy had 2.11 and 4.79 times higher odds of being in a good mental health class relative to fair and poor mental health classes. Respondents with more than two acute symptoms had 3.85 times higher odds of being in a good mental health class relative to a poor mental health class, as compared to those without acute symptoms. Regarding the measurement of the Perceived Social Support Scale (MSPSS), people having support from their family had 1.80 and 2.23 times higher odds of being in classes of fair and good mental health relative to the poor mental health class; and participants having friend support also had 1.87 times higher odds of being in a good mental health class compared with the fair mental health class, as the MSPSS score increased by 1 unit. People with Rosenberg’s self-esteem scale increased by 1 score, those who had 1.17, 1.26, and 1.47 times higher odds of being in a good compared to fair mental health class, fair compared to poor mental health class, and good compared to poor mental health class, respectively.

Conclusion

Our findings were given to promote a new classification method for mental health screening among the general population. The current findings could be used as evidence to develop policies and plans that focus on encouraging early screening for mental health problems among the general young population in the future.
Alternative Object Use in Adults and Children: Embodied Cognitive Bases of Creativity
Authors: Gubenko Alla , Houssemand Claude
Published: September 2022

Why does one need creativity? On a personal level, improvisation with available resources is needed for online coping with unforeseen environmental stimuli when existing knowledge and apparent action strategies do not work. On a cultural level, the exploitation of existing cultural means and norms for the deliberate production of novel and valuable artifacts is a basis for cultural and technological development and extension of human action possibilities across various domains. It is less clear, however, how creativity develops and how exactly one arrives at generating new action possibilities and producing multiple alternative action strategies using familiar objects. In this theoretical paper, we first consider existing accounts of the creative process in the Alternative Uses Task and then present an alternative interpretation, drawing on sociocultural views and an embodied cognition approach. We explore similarities between the psychological processes underlying the generation of new uses in the Alternative Uses Task and children’s pretend play. We discuss possible cognitive mechanisms and speculate how the generation of new action possibilities for common objects in pretend play can be related to adults’ ability to generate new action strategies associated with object use. Implications for creativity development in humans and embodied artificial agents are discussed.
The own-voice benefit for word recognition in early bilinguals
Authors: Cheung Sarah , Babel Molly
Published: September 2022

The current study examines the self-voice benefit in an early bilingual population. Female Cantonese–English bilinguals produced words containing Cantonese contrasts. A subset of these minimal pairs was selected as stimuli for a perception task. Speakers’ productions were grouped according to how acoustically contrastive their pronunciation of each minimal pair was and these groupings were used to design personalized experiments for each participant, featuring their own voice and the voices of others’ similarly-contrastive tokens. The perception task was a two-alternative forced-choice word identification paradigm in which participants heard isolated Cantonese words, which had undergone synthesis to mask the original talker identity. Listeners were more accurate in recognizing minimal pairs produced in their own (disguised) voice than recognizing the realizations of speakers who maintain similar degrees of phonetic contrast for the same minimal pairs. Generally, individuals with larger phonetic contrasts were also more accurate in word identification for self and other voices overall. These results provide evidence for an own-voice benefit for early bilinguals. These results suggest that the phonetic distributions that undergird phonological contrasts are heavily shaped by one’s own phonetic realizations.
A landmark-based approach to locate symptom-specific transcranial magnetic stimulation targets of depression
Authors: Du Rongrong , Zhou Qian , Hu Tianzheng , Sun Jinmei , Hua Qiang , Wang Yingru , Zhang Yuanyuan , He Kongliang , Tian Yanghua , Ji Gong-Jun , Wang Kai
Published: September 2022

Objective

Two subregions of the dorsolateral prefrontal cortex have been identified as effective repetitive transcranial magnetic stimulation (rTMS) targets for the “anxiosomatic” and “dysphoric” symptoms, respectively. We aimed to develop a convenient approach to locate these targets on the scalp.

Materials and methods

In a discovery experiment, the two personalized targets were precisely identified on 24 subjects using a neuronavigation system. Then, a localized approach was developed based on individual scalp landmarks. This “landmark-based approach” was replicated and validated in an independent cohort (N = 25). Reliability of the approach was tested by calculating the correlation of both the inter-rater and intra-rater results. Validity was tested by comparing the mean distance between the personalized and landmark-based targets to the TMS spatial resolution (i.e., 5 mm). We further conducted a total of 24 sham rTMS sessions to estimate the misplacement between the coil center and target during a 10-min stimulation without neuronavigation.

Results

The parameters of the “landmark-based approach” in the discovery experiment were replicated well in an independent cohort. Using discovery parameters, we successfully identified the symptom-specific targets in the independent cohort. Specifically, the mean distance between the personalized and landmark-based targets on the cortex was not significantly larger than 5 mm. However, the personalized and landmark-based targets distance exceeded 5 mm in more than 50% of subjects. During the 10-min sham rTMS session, the average coil misplacement was significantly larger than 5 mm.

Conclusion

The “landmark-based approach” can conveniently and reliably locate the two symptom-specific targets at group level. However, the accuracy was highly varied at individual level and further improvement is needed.
Use of remote data collection methodology to test for an illusory effect on visually guided cursor movements
Authors: Langridge Ryan W. , Marotta Jonathan J.
Published: September 2022

Investigating the influence of perception on the control of visually guided action typically involves controlled experimentation within the laboratory setting. When appropriate, however, behavioral research of this nature may benefit from the use of methods that allow for remote data collection outside of the lab. This study tested the feasibility of using remote data collection methods to explore the influence of perceived target size on visually guided cursor movements using the Ebbinghaus illusion. Participants completed the experiment remotely, using the trackpad of their personal laptop computers. The task required participants to click on a single circular target presented at either the left or right side of their screen as quickly and accurately as possible (Experiment 1), or to emphasize speed (Experiment 2) or accuracy (Experiment 3). On each trial the target was either surrounded by small or large context circles, or no context circles. Participants’ judgments of the targets’ perceived size were influenced by the illusion, however, the illusion failed to produce differences in click-point accuracy or movement time. Interestingly, the illusion appeared to affect participants’ movement of the cursor toward the target; more directional changes were made when clicking the Perceived Large version of the illusion compared to the Perceived Small version. These results suggest the planning of the cursor movement may have been influenced by the illusion, while later stages of the movement were not, and cursor movements directed toward targets perceived as smaller required less correction compared to targets perceived as larger.
Does a look of fear prompt to act? The effects of gaze and face emotional expression on manipulable objects
Authors: Scerrati Elisa , Rubichi Sandro , Iani Cristina
Published: September 2022

Gaze direction is an important social cue for understanding the intentions of other people. Indeed, interacting with others requires the ability to encode their current focus of attention in order to predict their future actions. Previous studies have showed that when asked to detect or identify a target, people are faster if shown a gaze oriented toward rather than away from that target. Most importantly, there is evidence that the emotion conveyed by the face with the averted gaze matters. We further tested the interplay between gaze and face emotion in the context of manipulable objects to understand whether and to what extent other people's gaze influences our own actions toward objects. Participants judged whether a target graspable object was upright or inverted after viewing a face cue with a central or averted gaze. Importantly, the target's handle could be oriented toward the gazed-at location or the opposite side such that gaze and handle were corresponding or non-corresponding in space. Furthermore, we manipulated the expression of the cue by using neutral and fearful faces. Results showed a handle-response (H-R) compatibility effect (i.e., a facilitation when the response key is on the same side as the object's handle) only with fearful cues with a central gaze.
Thinking about Kindergarten thinking: A mixed methods study
Authors: Braund Heather
Published: September 2022

Metacognition, otherwise known as ‘thinking about one’s thinking,’ leads to greater academic success and is foundational. Given this importance, metacognitive behaviors need to be developed within early years contexts to provide young children the opportunity to practice these behaviors and receive feedback. However, literature continues to focus on the development of metacognition in later grades. This mixed methods study explored metacognition in eight Kindergarten classrooms. Participants included eight Kindergarten teachers, six early childhood educators (ECEs), and 80 students. Data collection was conducted at two time periods separated by 12 weeks. Data collection included the Children’s Independent Learning Development (CHILD) measure, semi-structured interviews, and classroom observations. The quantitative data from the CHILD were analyzed using a paired samplest-test in SPSS. All qualitative data were analyzed thematically. Qualitatively, three themes were identified: (1) Conceptualization of metacognition, (2) Barriers to developing metacognition, and (3) Operationalization of strategies to facilitate metacognitive development. Evidence demonstrated that participants had incomplete conceptualizations of metacognition. Some articulated simple understandings by reporting the literal translation and were unable to articulate more fulsome conceptions. However, some teachers had more developed conceptions of metacognition that included different facets such as planning and reflective thinking. All participants were forthcoming with identifying ways in which they struggled with implementing metacognitive practices or encouraging the development of metacognition. These barriers included large classroom sizes, developmental readiness, and wide student ability. Despite having an incomplete understanding of metacognition, early years educators were trying a variety of different strategies to help promote metacognitive thinking within their Kindergarten classrooms. Surprisingly, teacher ratings using the CHILD did not change significantly from Time 1 (M= 1.88, SD = 0.744) to Time 2 (M= 1.85, SD = 0.66),t(72) = 0.72,p> 0.05. A similar trend was observed for ECE ratings using the CHILD as they did not change significantly from Time 1 (M= 1.89, SD = 0.70) to Time 2 (M= 1.80, SD = 0.79),t(52) = 1.36,p> 0.05. This research highlights empirical practices that Kindergarten educators can use to help facilitate metacognitive thinking. Furthermore, it identifies a need to better support Kindergarten educators by integrating practices aimed at developing metacognitive thinking in their students through explicit examples of strategies.
The Mediating Roles of Attitude Toward COVID-19 Vaccination, Trust in Science and Trust in Government in the Relationship Between Anti-vaccine Conspiracy Beliefs and Vaccination Intention
Authors: Capasso Miriam , Caso Daniela , Zimet Gregory D.
Published: September 2022

Since the outbreak of COVID-19, many conspiracy theories have spread widely, which has the potential to reduce adherence to recommended preventive measures. Specifically, anti-vaccine conspiracy beliefs can have a strong negative impact on COVID-19 vaccination attitude and intention. The present study aimed to clarify how such beliefs can reduce vaccination intention, exploring the possible mediating roles of attitude toward vaccination, trust in science, and trust in government, among a sample of 822 unvaccinated Italian adults (Women = 67.4%; Mage = 38.1). Path analysis showed that anti-vaccine conspiracy beliefs influenced intention to get vaccinated both directly and indirectly through the mediating effects of attitude, trust in science, and trust in government. In particular, the simple mediating effect of attitude was the strongest one, followed by the serial mediating effect of trust in science and attitude itself. Findings provide insights into the design of interventions aimed at reducing misinformation and subsequent vaccine hesitancy.
Students’ adaptive deep learning path and teaching strategy of contemporary ceramic art under the background of Internet +
Authors: Zhang Rui , Yao Xianjing , Ye Lele , Chen Min
Published: September 2022

With the rapid expansion of Internet technology, this research aims to explore the teaching strategies of ceramic art for contemporary students. Based on deep learning (DL), an automatic question answering (QA) system is established, new teaching strategies are analyzed, and the Internet is combined with the automatic QA system to help students solve problems encountered in the process of learning. Firstly, the related theories of DL and personalized learning are analyzed. Among DL-related theories, Back Propagation Neural Network (BPNN), Convolutional Neural Network (CNN), Long Short-Term Memory (LSTM), and Gated Recurrent Unit (GRU) are compared to implement a single model and a mixed model. Secondly, the collected student questions are selected and processed, and experimental parameters in different models are set for comparative experiments. Experiments reveal that the average accuracy and Mean Reciprocal Rank (MRR) of traditional retrieval methods can only reach about 0.5. In the basic neural network, the average accuracy of LSTM and GRU structural models is about 0.81, which can achieve better results. Finally, the accuracy of the hybrid model can reach about 0.82, and the accuracy and MRR of the Bidirectional Gated Recurrent Unit Network-Attention (BiGRU-Attention) model are 0.87 and 0.89, respectively, achieving the best results. The established DL model meets the requirements of the online automatic QA system, improves the teaching system, and helps students better understand and solve problems in the ceramic art courses.