bluuebunny/crossref_metadata_2025 huggingface dataset:
Morphine Antinociception Restored by Use of Methadone in the Morphine-Resistant Inflammatory Pain State
Authors: Watanabe Chizuko , Komiyama Asami , Yoshizumi Masaru , Sakurada Shinobu , Mizoguchi Hirokazu
Published: December 2020

The antinociceptive effect of methadone in the morphine-resistant inflammatory pain state was described in the paw-withdrawal test using the complete Freund’s adjuvant (CFA)-induced mouse inflammatory pain model. After intraplantar (i.pl.) injection of CFA, thermal hyperalgesia was observed in the ipsilateral paw. The antinociceptive effects of subcutaneous (s.c.) injection of morphine, fentanyl, and oxycodone against thermal hyperalgesia in the inflammatory pain state were reduced in the ipsilateral paw 7 days after CFA pretreatment. On the contrary, the antinociceptive effect of s.c. injection of methadone was maintained in the ipsilateral paw 7 days after CFA pretreatment. The suppressed morphine antinociception in the CFA model mice was bilaterally restored following s.c. treatment with methadone 20 min prior to or 3 days after CFA pretreatment. The suppressed morphine antinociception was also bilaterally restored by intraperitoneal treatment with MK-801 30 min prior to CFA pretreatment; however, the s.c. injection of morphine 30 min prior to CFA pretreatment failed to restore the suppressed morphine antinociception in the CFA model mice. The expression level of mRNA for µ-opioid receptors 7 days after i.pl. pretreatment was not significantly changed by i.pl. pretreatment with CFA or s.c. pretreatment with methadone. In conclusion, methadone is extremely effective against thermal hyperalgesia in the morphine-resistant inflammatory pain state, and restores suppressed morphine antinociception in the inflammatory pain state without altering the expression level of mRNA for µ-opioid receptors.
Network Pharmacology-Based Analysis of Xiao-Xu-Ming Decoction on the Treatment of Alzheimer’s Disease
Authors: Shen Yanjia , Zhang Baoyue , Pang Xiaocong , Yang Ran , Chen Miao , Zhao Jiaying , Wang Jinhua , Wang Zhe , Yu Ziru , Wang Yuehua , Li Li , Liu Ailin , Du Guanhua
Published: December 2020

Alzheimer's disease (AD) has become a worldwide disease that is harmful to human health and brings a heavy economic burden to healthcare system. Xiao-Xu-Ming Decoction (XXMD) has been widely used to treat stroke and other neurological diseases for more than 1000 years in China. However, the synergistic mechanism of the constituents in XXMD for the potential treatment of AD is still unclear. Therefore, the present study aimed to predict the potential targets and uncover the material basis of XXMD for the potential treatment of AD. A network pharmacology-based method, which combined data collection, drug-likeness filtering and absorption, distribution, metabolism, excretion and toxicity (ADME/T) properties filtering, target prediction and network analysis, was used to decipher the effect and potential targets of XXMD for the treatment of AD. Then, the acetylcholinesterase (AChE) inhibitory assay was used to screen the potential active constituents in XXMD for the treatment of AD, and the molecular docking was furtherly used to identify the binding ability of active constituents with AD-related target of AChE. Finally, three in vitro cell models were applied to evaluate the neuroprotective effects of potential lead compounds in XXMD. Through the China Natural Products Database, Traditional Chinese Medicine Systems Pharmacology (TCMSP) Database, Traditional Chinese Medicine (TCM)-Database @Taiwan and literature, a total of 1481 compounds in XXMD were finally collected. After ADME/T properties filtering, 908 compounds were used for the further study. Based on the prediction data, the constituents in XXMD formula could interact with 41 AD-related targets. Among them, cyclooxygenase-2 (COX-2), estrogen receptor α (ERα) and AChE were the major targets. The constituents in XXMD were found to have the potential to treat AD through multiple AD-related targets. 62 constituents in it were found to interact with more than or equal to 10 AD-related targets. The prediction results were further validated by in vitro biology experiment, resulting in several potential anti-AD multitarget-directed ligands (MTDLs), including two AChE inhibitors with the IC50 values ranging from 4.83 to 10.22 μM. Moreover, fanchinoline was furtherly found to prevent SH-SY5Y cells from the cytotoxicities induced by sodium nitroprusside, sodium dithionate and potassium chloride. In conclusion, XXMD was found to have the potential to treat AD by targeting multiple AD-related targets and canonical pathways. Fangchinoline and dauricine might be the potential lead compounds in XXMD for the treatment of AD.
Targeted Delivery of Curcumin to Polyethylene-Induced Osteolysis by Magnetically Guided Zoledronate-Anchored Poly Lactic-Co-Glycolic Acid Nanoparticles via Repressing NF-κB Signaling
Authors: Li Jingyi , Niu Chengcheng , Jiang Zichao , Zhang Zhen , Pan Yixiao , Xing Qiqi , Guo Qi , An Senbo , Hu Yihe , Wang Long
Published: December 2020

Aseptic loosening induced by periprosthetic osteolysis (PPO) is the leading complication of total joint arthroplasty (TJA) and results in patients having to receive revision surgery. However, there is still no efficient drug to prevent or even slow the pathological process. Herein, we report novel dual-targeted, curcumin-loaded Poly lactic-co-glycolic acid nanoparticles (ZSCNPs) to inhibit polyethylene-induced osteolysis. These ZSCNPs have good biocompatibility and excellent bone binding affinity. Under external magnetic field guidance, the ZSCNPs can specifically target osteolytic sites with sustained curcumin release, efficiently suppress the effect of IκB kinase, subsequently inhibit activation of the nuclear factor-kappa B (NF-κB) signaling pathway, and ultimately prevent osteoclast formation and particle-induced osteolysis. Therefore, these novel dual-targeted, drug-loaded nanoparticles could be applied as a useful strategy for targeted treatment of PPO after TJA.
Limestone Acoustic Emission Evolution Characteristics Under Different Experimental Loading and Unloading Conditions
Authors: Li Jielin , Hong Liu , Zhou Keping , Xia Caichu , Zhu Longyin
Published: December 2020

To study the acoustic emission evolution characteristics of saturated limestone under different loading and unloading paths, three cyclic loading and unloading tests were conducted with different loading rates and initial cyclic peak stresses, and acoustic emission monitoring was performed simultaneously. The results indicate that, during loading and unloading, the intermediate-frequency signals of saturated rock exhibit a variation trend of sparse–dense–sparse signals, whereas the low-frequency signals are continuously and massively produced. With the increase in the loading rate, the development trends of cumulative hits and energy become closer, and the development form of ringing count changes from N-type to U-type and then to N-type. The slight increase period and attenuation period are extended, whereas the intense growth period and postpeak calm period are shortened. With an increase in the initial cyclic peak stress, the change in cumulative energy is more obvious than that in cumulative hits near the rock failure. The development form of the ringing count changes from U-type to W-type and then to N-type, and each period is first shortened and then extended. With the increase in loading rate, the increase in the slow-change period tends to change from gradually increasing to increasing and then decreasing. By contrast, the increase in the step tends to change to a gradual increase. With the increase in the initial cyclic peak stress, the duration of and increase in the energy in the step and the slow-change period tend to decrease and then increase.
Histone Deacetylase 3-Mediated Inhibition of microRNA-19a-3p Facilitates the Development of Rheumatoid Arthritis-Associated Interstitial Lung Disease
Authors: Yuan Hui , Jiao Li , Yu Nan , Duan Haifeng , Yu Yong , Bai Yanrong
Published: December 2020

Histone deacetylase (HDAC) has been implicated in rheumatoid arthritis (RA) progression. We investigated the roles of histone deacetylase 3 (HDAC3) involved in RA-associated interstitial lung disease (ILD) fibrosis. Firstly, we measured the expression of HDAC3 and interleukin 17 receptor A (IL17RA) in lung tissue samples from normal controls, idiopathic pulmonary fibrosis (IPF) patients, and RA-ILD patients. Next, chromatin immunoprecipitation (ChIP) and dual luciferase reporter assay were employed to detect the interaction between HDAC3 and microRNA-19a-3p (miR-19a-3p) and between miR-19a-3p and IL17RA. Further, immunohistochemistry was used to localize HDAC3 and IL17RA expression in lung tissues. Additionally, functional assays were conducted followed by expression determination of HDAC3, miR-19a-3p, and IL17RA with reverse transcription quantitative PCR (RT-qPCR) and Western blot analysis. The effect of HDAC3 on RA-ILD in the constructed RA-ILD mouse model was also studied based on arthritis assessment. We found overexpressed HDAC3 and IL17RA as well as silenced miR-19a-3p in RA-ILD mouse model and RA-ILD patients. In the mouse model, HDAC3 downregulated miR-19a-3p in lung fibroblasts to promote the progression of RA-ILD fibrosis. In lung fibroblasts of RA-ILD mice, IL17RA was a target gene of miR-19a-3p. miR-19a-3p negatively regulated IL17RA, thereby increasing the expression of fibrosis markers, COL1A1, COL3A1, and FN, in lung fibroblasts of mice. Taken together, HDAC3 upregulated IL17RA expression by targeting miR-19a-3p to facilitate the RA-ILD fibrosis development, which sheds light on a new HDAC3/miR-19a-3p/IL17RA axis functioning in RA-ILD fibrosis.
Response of Basil Growth and Morphology to Light Intensity and Spectrum in a Vertical Farm
Authors: Larsen Dorthe H. , Woltering Ernst J. , Nicole Celine C. S. , Marcelis Leo F. M.
Published: December 2020

Vertical farming is becoming increasingly popular for production of leafy vegetables and herbs, with basil (Ocimum basilicum L.) as one of the most popular herbs. In basil most research has focused on increasing secondary metabolites with light spectra. However, knowledge about the effect of light intensity (photosynthetic photon flux density, PPFD) and spectra on growth and morphology is key for optimizing quality at harvest. The impact of PPFD and spectrum on plant growth and development is species dependent and currently few studies in basil are available. Understanding the response to End-Of-Production (EOP) light of growth and morphology is important for successful vertical farming. We performed a comprehensive series of experiments, where the effects of EOP PPFD, fraction of blue and their interaction on the growth and morphology were analyzed in two green and one purple basil cultivar. In addition, the impact of different EOP intensities and duration of far-red were investigated. We found that increasing the PPFD increased fresh mass, dry matter content and plant height in all three cultivars. The responses were linear or quadratic depending on the cultivar. A high fraction of blue (>90%) increased plant height and decreased the dry mass partitioning to the leaves. The only interaction found between the fraction of blue and overall PPFD was on plant height in the green cultivar whereas other growth parameters and morphology responded stronger to PPFD than to the fraction of blue light. Plant dry matter production was increased with the addition of far-red. Far-red EOP intensity treatments enhanced the fraction of dry mass partitioned to the leaves, whereas a prolonged far-red treatment enhanced partitioning to the stem. Both plant fresh mass and dry matter content were improved by applying high PPFD shortly before harvest. Light spectra were found to be of less importance than PPFD with respect to plant dry matter content. Light use efficiency (LUE) based on fresh mass decreased with increasing PPFD whereas LUE based on dry mass increased with increasing PPFD, when given as EOP treatments. The overall physiological mechanisms of the light intensity and spectral effects are discussed.
Comparison of MALDI-TOF-MS and RP-HPLC as Rapid Screening Methods for Wheat Lines With Altered Gliadin Compositions
Authors: Jang You-Ran , Cho Kyoungwon , Kim Sewon , Sim Jae-Ryeong , Lee Su-Bin , Kim Beom-Gi , Gu Yong Q. , Altenbach Susan B. , Lim Sun-Hyung , Goo Tae-Won , Lee Jong-Yeol
Published: December 2020

The wheat gliadins are a complex group of flour proteins that can trigger celiac disease and serious food allergies. As a result, mutation breeding and biotechnology approaches are being used to develop new wheat lines with reduced immunogenic potential. Key to these efforts is the development of rapid, high-throughput methods that can be used as a first step in selecting lines with altered gliadin contents. In this paper, we optimized matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and reversed-phase high-performance liquid chromatography (RP-HPLC) methods for the separation of gliadins from Triticum aestivum cv. Chinese Spring (CS). We evaluated the quality of the resulting profiles using the complete set of gliadin gene sequences recently obtained from this cultivar as well as a set of aneuploid lines in CS. The gliadins were resolved into 13 peaks by MALDI-TOF-MS. α- or γ-gliadins that contain abundant celiac disease epitopes and are likely targets for efforts to reduce the immunogenicity of flour were found in several peaks. However, other peaks contained multiple α- and γ-gliadins, including one peak with as many as 12 different gliadins. In comparison, separation of proteins by RP-HPLC yielded 28 gliadin peaks, including 13 peaks containing α-gliadins and eight peaks containing γ-gliadins. While the separation of α- and γ-gliadins gliadins achieved by RP-HPLC was better than that achieved by MALDI-TOF-MS, it was not possible to link peaks with individual protein sequences. Both MALDI-TOF-MS and RP-HPLC provided adequate separation of ω-gliadins. While MALDI-TOF-MS is faster and could prove useful in studies that target specific gliadins, RP-HPLC is an effective method that can be applied more broadly to detect changes in gliadin composition.
Gaining Longitudinal Accounts of Carers' Experiences Using IPA and Photograph Elicitation
Authors: Morrison Val , Williams Karina
Published: December 2020

Fluctuations in positive and negative caregiving experiences remain only partially explained as the significant variability over time of potential predictive factors themselves is understudied. The current study aims to gain considerable insight into caregiving experiences and perceptions over time by using photovoice methodology to support semi-structured interviews. A case study, longitudinal design is taken with three female caregivers who provide detailed insight into their caregivers' experiences over a 12 month period. The interview transcripts were analyzed using IPA- Interpretative Phenomenological Analysis. This innovative combination of methods resulted in the emergence of three related themes which included consuming the role, feeling consumed by the role, and letting go of the role. The idiographic approach taken allowed both within case differences to be examined over time, and also between carer differences to be highlighted. Implications of illness type and its characteristics, and of attachment and relationship quality with the care recipient were seen in terms of how and when the caregivers moved between the themes identified. The use of others' support or respite care is examined vis-a vis caregiver's own beliefs, emotions, relationship attachment and motivations to care. Caregivers self-efficacy beliefs also shifted over time and were influential in caregiver experience as the care recipient condition or needs changed. No previous studies have found that negative caregiving consequences are, in part, under volitional control and yet our data on the underlying reasons for consuming caregiving or allowing themselves to consume, would suggest this may in part be true. This is important because it suggests that interventions to support caregivers should address relational and motivational factors more fully.
Exploring the Relationship Between Parental Involvement, Paper Folding Skills, and Early Spatial Ability: A Mediation Model
Authors: Wu Dandan , Sun Jin
Published: December 2020

Paper folding is a common activity in East Asian kindergartens, but its potential value to early spatial skills have not been empirically explored. This study aims to investigate whether and how paper folding skills can predict spatial ability (SA) in the early years. Altogether 101 preschoolers (Ngirl = 45, Mage = 4.54, SD = 0.75) were randomly sampled from two Hong Kong kindergartens and invited to complete the map-use and the paper folding tasks. The paper folding task taps two levels of children’s paper folding skills: Basic Folding Skill (BFS) and Advanced Folding Skill (AFS). The parents reported the demographic information and their involvement in spatial activities at home. The results indicated the following: (1) there was a significant age-related increase in the paper folding performance; (2) child age could significantly predict both BFS (β = 0.551, p < 0.001) and AFS (β = 0.627, p < 0.001), while parental involvement could only predict BFS (β = 0.246, p < 0.001); (3) after controlling for confounders, paper folding skills could significantly predict SA as measured by the map-use task; (4) BFS was found to mediate the relationship between parental involvement and SA. The educational implications of these findings are also discussed.
Method Development for Multimodal Data Corpus Analysis of Expressive Instrumental Music Performance
Authors: Visi Federico Ghelli , Östersjö Stefan , Ek Robert , Röijezon Ulrik
Published: December 2020

Musical performance is a multimodal experience, for performers and listeners alike. This paper reports on a pilot study which constitutes the first step toward a comprehensive approach to the experience of music as performed. We aim at bridging the gap between qualitative and quantitative approaches, by combining methods for data collection. The purpose is to build a data corpus containing multimodal measures linked to high-level subjective observations. This will allow for a systematic inclusion of the knowledge of music professionals in an analytic framework, which synthesizes methods across established research disciplines. We outline the methods we are currently developing for the creation of a multimodal data corpus dedicated to the analysis and exploration of instrumental music performance from the perspective of embodied music cognition. This will enable the study of the multiple facets of instrumental music performance in great detail, as well as lead to the development of music creation techniques that take advantage of the cross-modal relationships and higher-level qualities emerging from the analysis of this multi-layered, multimodal corpus. The results of the pilot project suggest that qualitative analysis through stimulated recall is an efficient method for generating higher-level understandings of musical performance. Furthermore, the results indicate several directions for further development, regarding observational movement analysis, and computational analysis of coarticulation, chunking, and movement qualities in musical performance. We argue that the development of methods for combining qualitative and quantitative data are required to fully understand expressive musical performance, especially in a broader scenario in which arts, humanities, and science are increasingly entangled. The future work in the project will therefore entail an increasingly multimodal analysis, aiming to become as holistic as is music in performance.
Safe but Lonely? Loneliness, Anxiety, and Depression Symptoms and COVID-19
Authors: Okruszek Łukasz , Aniszewska-Stańczuk Aleksandra , Piejka Aleksandra , Wiśniewska Marcelina , Żurek Karolina
Published: December 2020

Background

The COVID-19 pandemic has led governments worldwide to implement unprecedented response strategies. While crucial to limiting the spread of the virus, “social distancing” may lead to severe psychological consequences, especially in lonely individuals.

Methods

We used cross-sectional (n = 380) and longitudinal (n = 74) designs to investigate the links between loneliness, anxiety, and depression symptoms (ADS) and COVID-19 risk perception and affective response in young adults who implemented social distancing during the first 2 weeks of the state of epidemic threat in Poland.

Results

Loneliness was correlated with ADS and with affective response to COVID-19’s threat to health. However, increased worry about the social isolation and heightened risk perception for financial problems was observed in lonelier individuals. The cross-lagged influence of the initial affective response to COVID-19 on subsequent levels of loneliness was also found.

Conclusion

The reciprocal connections between loneliness and COVID-19 response may be of crucial importance for ADS during the COVID-19 crisis.
Psychological Resources Protect Well-Being During the COVID-19 Pandemic: A Longitudinal Study During the French Lockdown
Authors: Pellerin Nicolas , Raufaste Eric
Published: December 2020

This longitudinal study investigated the capability of various positive psychological resources to directly or indirectly protect specific well-being outcomes and moderate the effects on well-being of health and economic threats in a lockdown situation during the 2020 health crisis in France. At the beginning of lockdown (wave 1), participants (N = 470) completed self-assessment questionnaires to document their initial level of well-being and state of nine different well-established psychological resources, measured as traits: optimism, hope, self-efficacy, gratitude toward the world, self-transcendence, wisdom, gratitude of being, peaceful disengagement, and acceptance. Three weeks later, a weekly follow-up was started to record changes in well-being and reported threats for a duration of 5 weeks (waves 2–6). Results show that psychological resources efficiently protected well-being in a variety of ways: they buffered the adverse effects of reported threats to health and wealth, increased the well-being averages, and reduced the decline in well-being over time. More specifically, emotional well-being was positively predicted by hope, gratitude of being, and, to a lesser level, by acceptance; psychological well-being by self-efficacy, personal wisdom, and gratitude of being; social well-being only by gratitude toward the world; and inner well-being by optimism, gratitude of being, and acceptance. The study emphasizes the importance of cultivating psychological resources in ordinary times to protect individuals' well-being when difficult and extraordinary circumstances occur. It also offers clues to the kind of resources one may want to develop.
The Child Affective Facial Expression Set Short Versions (CAFE-Ss): Development and Validation of Two Subsets of Children’s Emotional Faces With Variability
Authors: Yang Yang , LoBue Vanessa
Published: December 2020

Emotion recognition plays an important role in children’s socio-emotional development. Research on children’s emotion recognition has heavily relied on stimulus sets of photos of adults posed stereotyped facial configurations. The Child Affective Facial Expression set (CAFE) is a relatively new stimulus set that provides researchers with photographs of a diverse group of children’s facial configurations in seven emotional categories—angry, sad, happy, fearful, disgusted, surprised, and neutral. However, the large size of the full CAFE set makes it less ideal for research in children. Here, we introduce two subsets of CAFE with 140 photographs of children’s facial configurations in each set, diverse in the race and ethnicity of the models, and designed to produce variability in naïve observers. The subsets have been validated with 1000 adult participants.
An Algorithmic Metaphysics of Self-Patterns
Authors: Beni Majid D.
Published: December 2020

The paper draws on an algorithmic criterion to demonstrate that the self (as initially described in Shaun Gallagher’s a pattern theory of self) is a composite, scattered, and patterned object. It also addresses the question of extendedness of the self-pattern. Based on the criteria drawn from algorithmic complexity, I argue that although the self-pattern possesses a genuinely extended aspect (and in this sense, the self-pattern is minimally extended) the self-pattern and its environment do not constitute a genuine composite object.
Comparative Effectiveness of Mild or Conventional GnRH-Antagonist Protocols for Ovarian Stimulation in Poor Responders (Poseidon Group 4)
Authors: Le Hoang , Nguyen Dong D. , Cao Anh T. , Nguyen Huong T. L. , Tham Dung C. , Le Thang D. , Hugues Jean-Noël
Published: December 2020

Background and Aims: A panel of experts (the Poseidon Group) introduced a new and more detailed stratification for poor ovarian responders in order to predict the prognosis of IVF outcome according to the sensitivity to FSH. However, various arguments about the management strategy of these patients still remain, including the convenience and the cost. Therefore, this study was conducted to compare the efficacy of mild and conventional GnRH antagonist ovarian stimulation prescribed in patients classified in Poseidon Group 4.

Methods: This retrospective cohort study included 359 poor responder patients (Poseidon Group 4) treated with mild or conventional GnRH antagonist stimulation regimens from 8/2017 to 7/2019 at Tam Anh Hospital ART Center. The main outcomes were the index of Follicular Output Rate (FORT) or Follicle to Oocyte Index (FOI), the number of day-2 embryos and top-quality embryos obtained. The t-test and Mann–Whitney U test in SPSS v25.0 was used to analyze the continuous data and Chi-squared/Exact test was used for binary variables. Multiple linear regression analysis was done by using Stata versions 15.0 to measure association between primary endpoints with stimulation regimen controlled for covariates and possible confounding factors.

Results: In the overall group of poor responders, the conventional GnRH antagonist protocol performed better than the mild protocol. Subsequently, data were analyzed according to the AFC. In women with AFC < 3, no significant differences were observed between the 2 regimens regarding FORT (p = 0.71), FOI (p = 0.12), the number of day-2-embryos (p = 0.052) and the number of top-quality embryos (p = 0.26). In contrast, in women with AFC ≥ 3, mild stimulation regimen resulted in significantly poorer outcome compared to the conventional GnRH antagonist regimen, regarding FORT (p < 0.01), FOI (p < 0.01), the number of day-2-embryos (p < 0.01) and top-quality embryos (p = 0.01).

Conclusions: Considering poor responders classified in Poseidon Group 4, both ovarian stimulation regimens resulted in similar outcome for patients with a very low ovarian reserve (AFC < 3). In contrast, the GnRH conventional antagonist protocol with maximum initial FSH dose (300–375 IU/day) and supplementary LH (75–150 IU/day) was more effective than the mild one for patients whose ovarian reserve was less reduced. The Clinical Trial was approved by the Ethnical Biomedical Research Committee Tam Anh General Hospital.
Some Notes on the Omega Distribution and the Pliant Probability Distribution Family
Authors: Vasileva Maria T.
Published: December 2020

In 2020 Dombi and Jónás (Acta Polytechnica Hungarica 17:1, 2020) introduced a new four parameter probability distribution which they named the pliant probability distribution family. One of the special members of this family is the so-called omega probability distribution. This paper deals with one of the important characteristic “saturation” of these new cumulative functions to the horizontal asymptote with respect to Hausdorff metric. We obtain upper and lower estimates for the value of the Hausdorff distance. A simple dynamic software module using CAS Mathematica and Wolfram Cloud Open Access is developed. Numerical examples are given to illustrate the applicability of obtained results.
Hydraulic Switching Control Supplementing Speed Variable Hydraulic Drives
Authors: Zagar Philipp , Kogler Helmut , Scheidl Rudolf , Winkler Bernd
Published: December 2020

Primary control of linear motion by variable speed electric motors driving a hydraulic cylinder via a constant displacement pump is an established and successful concept with a frequent use in industry. One problem arises when low or zero motion speed has to be realized under high pump pressure conditions. Such load scenarios occur frequently in certain pressing processes, e.g., for sintering or veneering. Most pumps have a lower speed limit, below which critical tribological conditions occur which impair lifespan and efficiency. In addition, pump speed control and pump fluctuation suffer from the mixed lubrication conditions in such an operation range. For a circumvention of such low speed pump operation, a digital valve control concept is presented and studied in this paper. Valve control is used in load holding phases with low speed. Pressure is provided by an accumulator which is charged by the pump in short charging cycles at reasonable pump speeds. It is shown that the mean control error during load holding phase lies within the desired band and the fluctuations of the control force are lower than those of the pump control. In addition, the unfavorable pump operation conditions can be avoided via digital control.
Effect of Type of Pregnancy on Transcriptional and Plasma Metabolic Response in Sheep and Its Further Effect on Progeny Lambs
Authors: Gallardo Paffetti María , Cárcamo Juan , Arias-Darraz Luis , Alvear Carlos , Ojeda Javier
Published: December 2020

The following study was performed in order to determine the effect of type of pregnancy on the transcriptional expression of genes that are engaged in angiogenesis and cell turnover/lactogenesis in the ewe mammary gland, evaluating its impact on the plasma metabolic response. In addition, an assessment of its further influence on plasma metabolic response, performance, and muscle transcriptional expression of lipogenic enzymes in progeny lambs was made. Thirteen Ile de France sheep (six twin- and seven single-bearing ewes) were allocated to graze ad libitum naturalized pasture from d 45 pre-partum to day 70 post-partum, while keeping their lambs on the same diet until day 60 after weaning. The samples were collected at different times and analyzed by qRT-PCR and plasma metabolic indicators. The data were processed using SPSS package. The results showed that twin-bearing ewes overexpressed VEGFR1 at birth, and BCL2 at birth and day 35 post-partum; however, single-bearing ewes overexpressed CAIV and IGF1 at day 35 post-partum. Similar metabolite concentrations in blood plasma were found between groups of ewes. The plasma metabolic response in lambs was similar between groups and it did not influence their performance, where a similar transcriptional expression of lipogenic enzymes in muscle was observed. Therefore, the type of pregnancy can explain the slight differences in mRNA expression that were found in angiogenesis and cell turnover/lactogenesis in mammary gland, although these differences not only did not affect the plasma metabolic response in ewes, but they also had no influence on plasma metabolic response, performance, and muscle transcriptional expression of their lambs.
Morphological and Molecular Characterization of Kochinema farodai Baqri and Bohra, 2001 (Dorylaimida: Nordiidae) from California, with the First Molecular Study and an Updated Taxonomy of the Genus
Authors: Álvarez-Ortega Sergio
Published: December 2020

This paper deals with the morphological and molecular characterization of Kochinema farodai Baqri and Bohra, 2001, with an integrative approach. The finding of K. faroidai in California is a remarkable biogeographical novelty, as it is the first American record of the species. Molecular data herein obtained represent the first molecular study of the genus Kochinema. Furthermore, scanning electron microscopy (SEM) observations of a member of Kochinema are provided for the first time. Additionally, this contribution provides new insights into the phylogeny and taxonomy of the nematode genus Kochinema. A brief historical outline of the matter is presented. Then, the morphological pattern of the genus is revised and illustrated, the anterior position of amphids, whose opening is located on lateral lip, being its most relevant diagnostic feature. The phylogenetic analysis inferred from D2–D3 expansion segments of 28S rRNA gene shows that Kochinema clustered together with other dorylaimid species characterized by the absence of pars refringens vaginae and that it does not share a recent common ancestor with other members of the family Nordiidae. A likely polyphyly of the family Nordiidae is confirmed. Finally, an updated taxonomy of the genus is proposed, including a revised diagnosis, a list of species, a key to species identification, and a compendium of their main morphometrics and distribution data.
Synergistic Antimicrobial Activity of Supplemented Medical-Grade Honey against Pseudomonas aeruginosa Biofilm Formation and Eradication
Authors: Pleeging Carlos C. F. , Coenye Tom , Mossialos Dimitris , de Rooster Hilde , Chrysostomou Daniela , Wagener Frank A. D. T. G. , Cremers Niels A. J.
Published: December 2020

Biofilms hinder wound healing. Medical-grade honey (MGH) is a promising therapy because of its broad-spectrum antimicrobial activity and the lack of risk for resistance. This study investigated the inhibitory and eradicative activity against multidrug-resistant Pseudomonas aeruginosa biofilms by different established MGH-based wound care formulations. Six different natural wound care products (Medihoney, Revamil, Mebo, Melladerm, L-Mesitran Ointment, and L-Mesitran Soft) were tested in vitro. Most of them contain MGH only, whereas some were supplemented. L-Mesitran Soft demonstrated the most potent antimicrobial activity (6.08-log inhibition and 3.18-log eradication). Other formulations ranged between 0.89-log and 4.80-log inhibition and 0.65-log and 1.66-log eradication. Therefore, the contribution of different ingredients of L-Mesitran Soft was investigated in more detail. The activity of the same batch of raw MGH (1.38-log inhibition and 2.35-log eradication), vitamins C and E (0.95-log inhibition and 0.94-log eradication), and all ingredients except MGH (1.69-log inhibition and 0.75-log eradication) clearly support a synergistic activity of components within the L-Mesitran Soft formulation. Several presented clinical cases illustrate its clinical antimicrobial efficacy against Pseudomonas aeruginosa biofilms. In conclusion, MGH is a potent treatment for Pseudomonas biofilms. L-Mesitran Soft has the strongest antimicrobial activity, which is likely due to the synergistic activity mediated by its supplements.
Anti-Viral Potential and Modulation of Nrf2 by Curcumin: Pharmacological Implications
Authors: Rahban Mahdie , Habibi-Rezaei Mehran , Mazaheri Mansoureh , Saso Luciano , Moosavi-Movahedi Ali A.
Published: December 2020

Nuclear factor erythroid 2-related factor 2 (Nrf2) is an essential transcription factor that maintains the cell’s redox balance state and reduces inflammation in different adverse stresses. Under the oxidative stress, Nrf2 is separated from Kelch-like ECH-associated protein 1 (Keap1), which is a key sensor of oxidative stress, translocated to the nucleus, interacts with the antioxidant response element (ARE) in the target gene, and then activates the transcriptional pathway to ameliorate the cellular redox condition. Curcumin is a yellow polyphenolic curcuminoid from Curcuma longa (turmeric) that has revealed a broad spectrum of bioactivities, including antioxidant, anti-inflammatory, anti-tumor, and anti-viral activities. Curcumin significantly increases the nuclear expression levels and promotes the biological effects of Nrf2 via the interaction with Cys151 in Keap1, which makes it a marvelous therapeutic candidate against a broad range of oxidative stress-related diseases, including type 2 diabetes (T2D), neurodegenerative diseases (NDs), cardiovascular diseases (CVDs), cancers, viral infections, and more recently SARS-CoV-2. Currently, the multifactorial property of the diseases and lack of adequate medical treatment, especially in viral diseases, result in developing new strategies to finding potential drugs. Curcumin potentially opens up new views as possible Nrf2 activator. However, its low bioavailability that is due to low solubility and low stability in the physiological conditions is a significant challenge in the field of its efficient and effective utilization in medicinal purposes. In this review, we summarized recent studies on the potential effect of curcumin to activate Nrf2 as the design of potential drugs for a viral infection like SARS-Cov2 and acute and chronic inflammation diseases in order to improve the cells’ protection.
Standardized Bacopa monnieri Extract Ameliorates Learning and Memory Impairments through Synaptic Protein, Neurogranin, Pro-and Mature BDNF Signaling, and HPA Axis in Prenatally Stressed Rat Offspring
Authors: Sivasangari Karunanithi , Rajan Koilmani Emmanuvel
Published: December 2020

Prenatal stress (PNS) influences offspring neurodevelopment, inducing anxiety-like behavior and memory deficits. We investigated whether pretreatment of Bacopa monnieri extract (CDRI-08/BME) ameliorates PNS-induced changes in signaling molecules, and changes in the behavior of Wistar rat offspring. Pregnant rats were randomly assigned into control (CON)/prenatal stress (PNS)/PNS and exposed to BME treatment (PNS + BME). Dams were exposed to stress by placing them in a social defeat cage, where they observed social defeat from gestational day (GD)-16–18. Pregnant rats in the PNS + BME group were given BME treatment from GD-10 to their offspring’s postnatal day (PND)-23, and to their offspring from PND-15 to -30. PNS led to anxiety-like behavior; impaired memory; increased the level of corticosterone (CORT), adrenocorticotropic hormone, glucocorticoid receptor, pro-apoptotic Casepase-3, and 5-HT2C receptor; decreased anti-apoptotic Bcl-2, synaptic proteins (synaptophysin, synaptotagmin-1), 5-HT1A, receptor, phosphorylation of calmodulin-dependent protein kinase II/neurogranin, N-methyl-D-aspartate receptors (2A,2B), postsynaptic density protein 95; and conversion of pro and mature brain derived neurotropic factor in their offspring. The antioxidant property of BME possibly inhibiting the PNS-induced changes in observed molecules, anxiety-like behavior, and memory deficits. The observed results suggest that pretreatment of BME could be an effective coping strategy to prevent PNS-induced behavioral impairments in their offspring.
In Vitro and In Vivo Inhibitory Effect of Citrus Junos Tanaka Peel Extract against Oxidative Stress-Induced Apoptotic Death of Lung Cells
Authors: Kim Jin Woo , Jo Eun Hee , Moon Ji Eun , Cha Hanvit , Chang Moon Han , Cho Hyung Taek , Lee Min Kook , Jung Wan Sik , Lee Jin Hyup , Heo Wan , Kim Young Jun
Published: December 2020

Various stresses derived from both internal and external oxidative environments lead to the excessive production of reactive oxygen species (ROS) causing progressive intracellular oxidative damage and ultimately cell death. The objective of this study was to evaluate the protective effects of Citrus junos Tanaka peel extract (CE) against oxidative-stress induced the apoptosis of lung cells and the associated mechanisms of action using in vitro and in vivo models. The protective effect of CE was evaluated in vitro in NCI-H460 human lung cells exposed to pro-oxidant H2O2. The preventive effect of CE (200 mg/kg/day, 10 days) against pulmonary injuries following acrolein inhalation (10 ppm for 12 h) was investigated using an in vivo mouse model. Herein, we demonstrated the inhibitory effect of CE against the oxidative stress-induced apoptosis of lung cells under a highly oxidative environment. The function of CE is linked with its ability to suppress ROS-dependent, p53-mediated apoptotic signaling. Furthermore, we evaluated the protective role of CE against apoptotic pulmonary injuries associated with the inhalation of acrolein, a ubiquitous and highly oxidizing environmental respiratory pollutant, through the attenuation of oxidative stress. The results indicated that CE exhibits a protective effect against the oxidative stress-induced apoptosis of lung cells in both in vitro and in vivo models.
Impact of the Enamel Cleaning Procedure during Debonding on Endodontium Temperature: In Vitro Tests
Authors: Machoy Monika , Szyszka-Sommerfeld Liliana , Duda Piotr , Wawrzyk Anna , Woźniak Krzysztof , Wilczyński Sławomir
Published: December 2020

Interference with live tooth tissue during dental treatment affects the temperature within the pulp. The pulp is sensitive to temperature changes, which can cause its inflammation. The aim of this study was to analyze the dynamics of pulp chamber temperature changes in response to the enamel cleaning procedure after orthodontic treatment. In the presented in vitro studies, by using a thermal imaging camera, the change in the temperature of the vestibular wall of the pulp chamber of the incisors and premolars was assessed as a function of time under the influence of polishing the enamel with the silicone rubber and aluminum oxides used during the debonding procedure after completion of orthodontic treatment with fixed appliances. The relationship between dentin density and enamel from changing the chamber temperature was evaluated by using Micro computed tomography, microtomography (micro-CT). The maximum achieved tooth surface temperature during polishing was 52.34 °C without water cooling and 43.15 °C using water cooling. The time after which a safe pulp temperature of 40 °C was obtained without water cooling was 29.4 s, while the time with water cooling was 34.6 s. The correlation between the maximum and average temperature achieved and the density of the teeth was analyzed based on micro-CT scans. No correlation between enamel or dentin density and rise in temperature was found.
Meshing Stiffness—A Parameter Affecting the Emission of Gearboxes
Authors: Maláková Silvia , Puškár Michal , Frankovský Peter , Sivák Samuel , Palko Maroš , Palko Miroslav
Published: December 2020

Emission is one of the key problems in the automotive industry, which engineers try to eliminate by lowering emissions to the minimum. Transmission emission plays an important part here. The basic characteristics of gears include their shape, load capacity, and emissions. The most significant source of noise and vibration in the gearbox is the step change in the meshing stiffness of the gearing, which depends on the path of meshing at the entry and exit of the meshed teeth. Ensuring a permanent multi-pair mesh is a way to mitigate these step changes as much as possible. This leads to the design and implementation of gears in an integer contact ratio. In addition to this, the article deals with the impact of individual parameters on the stiffness of the gearing, which is a source of noise and vibration. The meshing stiffness of the gearing was determined on the deformation basis of the gearing, as solved by the Finite Element Method.
Hyperlipidemic Rabbit Models for Anti-Atherosclerotic Drug Development
Authors: Niimi Manabu , Chen Yajie , Yan Haizhao , Wang Yao , Koike Tomonari , Fan Jianglin
Published: December 2020

Hyperlipidemia or dyslipidemia is a major risk factor for atherosclerotic diseases. Experimental animals play an important role in elucidating the molecular mechanisms of the pathophysiology of hyperlipidemia as well as in drug development. Rabbits are one of the most suitable models to study human hyperlipidemia because many features of the lipoprotein metabolism of rabbits are similar to those of humans such as LDL-rich lipoproteins in plasma, apolipoprotein B mRNA editing, and cholesteryl ester transfer protein. Currently, three types of rabbit models are commonly used for studying hyperlipidemia: (1) diet-induced hyperlipidemic rabbits, (2) spontaneous hyperlipidemic rabbits, and (3) gene-manipulated rabbits (transgenic and knockout rabbits). In this review, we give an overview of the features of hyperlipidemic rabbits and discuss the usefulness of rabbits for the development of anti-atherogenic drugs.
Efficient Regression Testing of Software Product Lines by Reducing Redundant Test Executions
Authors: Jung Pilsu , Kang Sungwon , Lee Jihyun
Published: December 2020

Regression testing for software product lines (SPLs) is challenging because it must ensure that all the products of a product family work correctly whenever changes are made. One approach to reducing the cost of regression testing is the regression test selection (RTS), which selects a subset of regression test cases. However, even when RTS is applied, SPL regression testing can still be expensive because, in the product line context, each test case can be executed on more than one product that reuses the test case, which would typically result in a large number of test executions. A promising direction is to eliminate redundant test executions of test cases. We propose a method that, given a test case, identifies a set of products, on which the test case will cover the same sequence of source code statements and produce the same testing results, and then excludes these products from products to apply the test case to. The evaluation results showed that when the full selection approach and the approach of repetitively applying an RTS method for a single software system are used for test selection, our method reduced, respectively, 59.3% and 40.0% of the numbers of test executions of the approaches.
Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) Coupled with Chemometrics, to Control the Botanical Authenticity and Quality of Cold-Pressed Functional Oils Commercialized in Romania
Authors: Socaciu Carmen , Fetea Florinela , Ranga Floricuta , Bunea Andrea , Dulf Francisc , Socaci Sonia , Pintea Adela
Published: December 2020

Attenuated total reflectance-Fourier transform infrared ppectroscopy (ATR-FTIR) proved to be a reliable, rapid, and easy-to-use technique to evaluate vegetable oils quality and authenticity. The spectral range of the middle infrared region (MIR) of FTIR spectra, from 4000 to 600 cm−1, has been commonly used to fingerprint specific functional groups of lipids and their modified forms induced by oxidation of thermal treatment. The applicability of FTIR-MIR spectroscopy in assessing oil fingerprinting and quality parameters is crucially dependent on the chemometric methods, including calibrations with authentic samples. We report here the evaluation of seven types of cold-pressed functional oils (sunflower, pumpkin, hempseed, soybean, walnut, linseed, sea buckthorn) produced in Romania, provided directly from small enterprises (as genuine, process-controlled authentic samples) comparative to commercialized samples. Concomitantly, olive oils of similar claimed quality were investigated. The ATR-FTIR-MIR data were complemented by UV–Vis spectral fingerprints and multivariate analysis using Unscrambler X.10.4 and Metaboanalyst 4.0 software (e.g., PCA, PLSDA, cluster analysis, heatmap, Random forest analysis) and ANOVA post-hoc analysis using Fischer’s least significant difference. The integration of spectral and chemometric analysis proved to offer valuable criteria for their botanical group recognition, individual authenticity, and quality, easy to be applied for large cohorts of commercialized oils.