bluuebunny/crossref_metadata_2025 huggingface dataset:
UAV Application for Short-Time Evolution Detection of the Vomice Landslide (South Italy)
Authors: Mercuri Michele , Conforti Massimo , Ciurleo Mariantonietta , Borrelli Luigi
Published: January 2023

This paper investigates the possibility to detect the short-time evolution of the slow-moving Vomice earth flow, located in the northeastern sector of the Calabria region (South Italy), by combining the information obtained from two different drone flights, carried out in February 2019 and June 2022, with field surveys. The obtained results consisted of delimiting all landslide bodies constituting the Vomice earth flow, detecting landslide types and the state of activity, as well as identifying spatial and volumetric changes. The obtained results showed that depletion and transition zones of the Vomice earth flow are active, while the accumulation zone appears prevalently dormant. Particularly, in the analyzed period, the depletion zone was characterized by local collapses of the main scarps where several slides evolving in earth flows caused more than 20 m of retrogressive fail upslope. The maximum elevation changes observed in these zones were about ±5 m. The volume of the material mobilized by mass movements was about 114.2 × 103 m3, whereas the volume of the accumulated material was approximately 92.7 × 103 m3. The transition zone was affected by several slow earthflows that re-mobilized the displaced material located in the middle portion of the landslide and reached the accumulation zone. Overall, the results of this study demonstrated the practicality and feasibility of using UAV tools for detecting the short-time evolution of a large landslide.
Is There a Correlation between Gingival Display and Incisal Inclination in a Gummy Smile? Study on Cephalometric Parameters
Authors: Impellizzeri Alessandra , Palmigiani Raissa , Horodynski Martina , D’alfonso Tiziana , Polimeni Antonella , De Stefano Adriana , Galluccio Gabriella
Published: January 2023

Background: Excessive gingival display or “gummy smile” is a clinical condition where a maxillary gum shows between the inferior line of the superior lip and the gingival line of the incisive superior during a spontaneous smile. The aim of this research was to understand the various skeletal and dentoalveolar components contributing to a gummy smile in a sample of 120 patients. Material and Methods: This retrospective case-control study had the primary objectives of analyzing the existence of a correlation between the presence of gingival exposure and the alteration of the inclination of the upper incisors with respect to the Frankfurt plane, the Palatine plane (bi-spinal) and to the NA line in a sample of orthodontic patients, and also evaluating the association with skeletal, dental, and aesthetic cephalometric parameters. Result and Conclusions: In our study, it’s emerged a correlation between the gingival exposure and the presence of alterations to incisal torque in the vestibular direction and the quantity of maxillary gingiva evident during the smile, which is correlated in particular to the Is–Sts distance, overjet and overbite. The major indicative data, therefore, are related to the vertical position of the upper incisors, in particular with respect to the upper lip and to the sagittal position.
The Ribadeo I Wreck—Multi-Year Photogrammetric Survey of a Spanish Galleon of the Second Armada
Authors: Mason Brandon , Heamagi Christin , Nayling Nigel
Published: January 2023

There are multiple approaches to bridging the gap between the relatively new world of three-dimensional survey and so-called reality-capture with traditional recording conventions and archaeological interpretative processes, challenges that have been encountered during the various fieldwork campaigns undertaken on the Ribadeo I wreck. Questions discussed in this paper include: does every team member need to be a 3D expert? Does a 3D specialist need to be an archaeologist? Is it enough to have a specialist 3D ‘navigator’, someone who can drive the software through the data and act as a guide to a wider team to deliver their own interpretation of the evidence? When conventional outputs are required, including plans, sections, profiles and other nautical-specific views, how can these be efficiently generated from the available photogrammetry and presented to provide comparable information to those who speak and require this type of visual language? This paper does not provide simple or definitive answers to these questions. However, the work undertaken so far on Ribadeo I can offer some contributions to current discussions and consider the challenges within the context of an internationally important shipwreck and work that is necessarily limited by available time and funds.
Development of Molecular-Based Species Identification and Optimization of Reaction Conditions for Molecular Diagnosis of Three Major Asian Planthoppers (Hemiptera: Delphacidae)
Authors: Rahman Md-Mafizur , Nam Hwayeun , Choi Nakjung , Kim Juil
Published: January 2023

Asian planthoppers (Hemiptera: Delphacidae) that include brown planthoppers (BPH, Nilaparvata lugens, Stål), white-backed planthoppers (WBPH, Sogatella furcifera, Horváth), and small brown planthoppers (SBPH, Laodelphax striatellus, Fallén) are the primary sucking-type pests of rice. These three insects share morphological and sequence similarities. As insecticide resistance patterns and control strategies vary according to species, the accurate discrimination of these species is important. Here, we developed six species-specific primers based on partial mitochondrial genome sequences. The primers were successfully used in multiplex PCR, loop-mediated isothermal amplification (LAMP) assays, and conventional PCR. Here, we used genomic DNA obtained using the DNA-releasing technique (tissue samples were incubated at 95 °C for 5 min with 30 μL nuclease-free water, and the supernatant was used). We showed that multiplex PCR could analyze the density of each species following a mass collection in the field; the LAMP assay can diagnose the species within 40 min; conventional PCR can be widely applied to a large number of field samples, as well as individuals or mass collections. In conclusion, these results demonstrate the potential of the species-specific primers and DNA-releasing technique for accurate multiplex PCR and LAMP assays, which may assist the intensive field monitoring of integrated management of these species.
How Families’ Use of Digital Technology Can Be a Tool for Reducing Loneliness and Improving Food Intake among Older Adults
Authors: Grini Ida Synnøve Bårvåg , Ueland Øydis
Published: January 2023

The purpose of this study was to explore how a technical solution implemented among older adults and connected with an app supervised by an app administrator can reduce loneliness, prevent malnutrition, and inspire social eating and networking. In October 2020, a survey was distributed to 3500 administrators of the one-button computer communication tool Komp. Komp consists of a screen placed with older adults and an app used by the administrator of the tool. The survey addresses aspects that can provide new insights into how older adults can use digital solutions as a link to family and external networks. The study results show that due to COVID-19, 65% of respondents said they used Komp more frequently than before, but only 5% of current use was associated with eating meals together. However, 54% of the app administrators indicated that this could be a good future activity. Furthermore, 88% thought Komp could contribute to more socializing through shared meals. This study elicited almost 1650 constructive comments on experience, use, and recommendations. The study results show that digital solutions can be a link between older adults and their families and external network. Such tools can address needs connected to loneliness, social isolation, and food intake.
Chronic Breathlessness in Obstructive Sleep Apnea and the Use of Lymphocyte Parameters to Identify Overlap Syndrome among Patients
Authors: Liu Dan , Wang Zhiding , Zhuang Yan , Wang Yan , Zhang Jing , Wang Rui , Cao Jie , Feng Jing
Published: January 2023

Little is known about the distribution of etiology in obstructive sleep apnea (OSA) combined with chronic breathlessness. A significant portion of patients in this group have so-called “overlap syndrome (OVS)”, characterized by chronic obstructive pulmonary disease (COPD). OVS has more complications and a poorer prognosis compared to patients with either OSA or COPD alone, which makes it important to identify OVS early in OSA. The current study was a retrospective cross-sectional analysis of consecutive adult patients who were diagnosed with OSA (n = 1062), of whom 275 were hospitalized due to chronic breathlessness. Respiratory and cardiac diseases accounted for the vast majority of causes, followed by gastrointestinal and renal disorders. The final study population comprised 115 patients with OSA alone (n = 64) and OVS (n = 51), who had chronic breathlessness as the primary complaint, not secondary as one of many other complaints. Lymphocytes, CD4 counts, neutrophil-to-lymphocyte ratio (NLR), and PLR were differently expressed between the OSA-alone group and OVS group. The NLR, lymphocytes, and CD4 counts had a moderate diagnostic value for OVS in OSA patients, with AUCs of 0.708 (95% CI, 0.614–0.802), 0.719 (95% CI, 0.624–0.813), and 0.744 (95% CI, 0.653–0.834), respectively. The NLR had the highest AUC for predicting a 6-month re-admission of OVS, with a cut-off of 3.567 and a moderate prognostic value. The sensitivity and specificity were 0.8 and 0.732, respectively. In the animal model, the spleen hematoxylin- and eosin-stained, electron microscopy images showed germinal-center damage, chromatin activation, and mitochondrial swelling under the overlapping effect of intermittent hypoxia and cigarette smoke exposure. OSA with chronic breathlessness cannot be overstated. A significant proportion of patients with COPD in this group had poor lung function at initial diagnosis. The NLR is a useful biomarker to differentiate OVS among OSA patients combined with chronic breathlessness.
Jacob’s Disease: Case Series, Extensive Literature Review and Classification Proposal
Authors: Raccampo Luca , Panozzo Giorgio , Tel Alessandro , Di Cosola Michele , Colapinto Gianluca , Trevisiol Lorenzo , D’agostino Antonio , Sembronio Salvatore , Robiony Massimo
Published: January 2023

Jacob’s disease is a rare entity consisting of the formation of a pseudojoint between an abnormal coronoid process of the mandible and the inner surface of the zygomatic bone. First described by Jacob in 1899, its diagnosis and definition have never been entirely univocal. In this paper, we present three emblematic cases and an extensive review of the literature on Jacob’s disease. Given the variability observed in the presentation of the disease, we have developed a proposal for the classification, here reported.
Temporomandibular Joint Osteoarthritis Diagnosis Employing Artificial Intelligence: Systematic Review and Meta-Analysis
Authors: Almășan Oana , Leucuța Daniel-Corneliu , Hedeșiu Mihaela , Mureșanu Sorana , Popa Ștefan Lucian
Published: January 2023

The aim was to systematically synthesize the current research and influence of artificial intelligence (AI) models on temporomandibular joint (TMJ) osteoarthritis (OA) diagnosis using cone-beam computed tomography (CBCT) or panoramic radiography. Seven databases (PubMed, Embase, Scopus, Web of Science, LILACS, ProQuest, and SpringerLink) were searched for TMJ OA and AI articles. We used QUADAS-2 to assess the risk of bias, while with MI-CLAIM we checked the minimum information about clinical artificial intelligence modeling. Two hundred and three records were identified, out of which seven were included, amounting to 10,077 TMJ images. Three studies focused on the diagnosis of TMJ OA using panoramic radiography with various transfer learning models (ResNet model) on which the meta-analysis was performed. The pooled sensitivity was 0.76 (95% CI 0.35–0.95) and the specificity was 0.79 (95% CI 0.75–0.83). The other studies investigated the 3D shape of the condyle and disease classification observed on CBCT images, as well as the numerous radiomics features that can be combined with clinical and proteomic data to investigate the most effective models and promising features for the diagnosis of TMJ OA. The accuracy of the methods was nearly equivalent; it was higher when the indeterminate diagnosis was excluded or when fine-tuning was used.
Association of Genetic Polymorphisms and Serum Levels of miR-1-3p with Postoperative Mortality following Abdominal Aortic Aneurysm Repair
Authors: Li Tan , Jiang Bo , Wu Yijun , Yang Jun , Ma Chunyan , Yuan Yuan
Published: January 2023

Background: Several miRNAs have been implicated in the clinical outcomes of cardiovascular disorders, but the role of miR-1-3p in abdominal aortic aneurysm (AAA) prognosis remains unclear. This study aimed to investigate the correlation of single nucleotide polymorphisms (SNPs) in pri-miR-1-3p and mature miR-1-3p expression with postoperative mortality of AAA patients. Methods: A total of 230 AAA patients who received AAA repair were recruited and followed up for 5 years. SNP genotyping was carried out using KASP method and relative expression of serum miR-1-3p was measured with qRT-PCR. Results: Multivariate Cox regression analyses showed that both rs2155975 and rs4591246 variant genotypes were associated with increased all-cause mortality of postoperative AAA patients after adjusting possible confounders. Patients who died tended to have lower baseline miR-1-3p expression (overall and for age < 65 years, aneurysm-related death or cardiac death subgroup) when compared to alive patients; further Cox regression yielded an independent relationship of preoperative low serum miR-1-3p levels with incidents of all-cause death. Patients carrying rs2155975 AG + GG or rs4591246 AG + AA genotype had a higher ratio of low miR-1-3p levels in contrast to those with AA or GG genotype, respectively. The Kaplan–Meier survival curves suggested that the combined genotype in rs2155975 or rs4591246 and low miR-1-3p levels could decrease the overall survival of AAA patients during 5-year follow-up. Conclusions: This pilot study demonstrated the importance of rs2155975 and rs4591246 polymorphisms and baseline serum miR-1-3p levels as promising markers to predict mortality among patients following AAA repair.
Differences and Similarities in Empathy Deficit and Its Neural Basis between Logopenic and Amnesic Alzheimer’s Disease
Authors: Giacomucci Giulia , Polito Cristina , Berti Valentina , Padiglioni Sonia , Galdo Giulia , Mazzeo Salvatore , Bergamin Enrico , Moschini Valentina , Morinelli Carmen , Nuti Claudia , De Cristofaro Maria Teresa , Ingannato Assunta , Bagnoli Silvia , Nacmias Benedetta , Sorbi Sandro , Bessi Valentina
Published: January 2023

The aims of the study were to assess empathy deficit and neuronal correlates in logopenic primary progressive aphasia (lv-PPA) and compare these data with those deriving from amnesic Alzheimer’s disease (AD). Eighteen lv-PPA and thirty-eight amnesic AD patients were included. Empathy in both cognitive and affective domains was assessed by Informer-rated Interpersonal Reactivity Index (perspective taking, PT, and fantasy, FT, for cognitive empathy; empathic concern, EC, and personal distress, PD, for affective empathy) before (T0) and after (T1) cognitive symptoms’ onset. Emotion recognition was explored through the Ekman 60 Faces Test. Cerebral FDG-PET was used to explore neural correlates underlying empathy deficits. From T0 to T1, PT scores decreased, and PD scores increased in both lv-PPA (PT z = −3.43, p = 0.001; PD z = −3.62, p < 0.001) and in amnesic AD (PT z = −4.57, p < 0.001; PD z = −5.20, p < 0.001). Delta PT (T0–T1) negatively correlated with metabolic disfunction of the right superior temporal gyrus, fusiform gyrus, and middle frontal gyrus (MFG) in amnesic AD and of the left inferior parietal lobule (IPL), insula, MFG, and bilateral superior frontal gyrus (SFG) in lv-PPA (p < 0.005). Delta PD (T0-T1) positively correlated with metabolic disfunction of the right inferior frontal gyrus in amnesic AD (p < 0.001) and of the left IPL, insula, and bilateral SFG in lv-PPA (p < 0.005). Lv-PPA and amnesic AD share the same empathic changes, with a damage of cognitive empathy and a heightening of personal distress over time. The differences in metabolic disfunctions correlated with empathy deficits might be due to a different vulnerability of specific brain regions in the two AD clinical presentations.
Contribution to the Personalized Management of the Nosocomial Infections: A New Paradigm Regarding the Influence of the Community Microbial Environment on the Incidence of the Healthcare-Associated Infections (HAI) in Emergency Hospital Surgical Departments
Authors: Mateescu Maria-Cristina , Grigorescu Simona , Socea Bogdan , Bloanca Vlad , Grigorescu Ovidiu-Dan
Published: January 2023

Background: The management of acute surgical pathology implies not only the diagnosis–treatment sequence but also an important preventive component. In the surgical hospital department, wound infection is one of the most frequent complications which must be managed both in a preventive and a personalized manner. To achieve this goal, several factors of negative local evolution, contributing to the slowdown of the healing processes, such as the colonization and contamination of the wounds, need to be emphasized and controlled from the first moment. In this context, knowing the bacteriological status at admission ensures the distinction between the colonization and infection processes and could help to manage in an efficient way the fight against bacterial pathogen infections from the beginning. Methods: A prospective study was performed for 21 months on 973 patients hospitalized as emergencies in the Plastic and Reconstructive Surgery Department within the Emergency University County Hospital of Brasov, Romania. We analyzed the bacteriological profile of the patients from admission to discharge and the bidirectional and cyclic microorganism dynamics both in the hospital and the community microbial environment. Results: Of the 973 samples collected at admission, 702 were positive, with 17 bacterial species and one fungal, with a predominance of Gram-positive cocci at 74,85%. The most frequently isolated strains were Staphylococcus species (86.51% of the Gram-positive/64.7% of the total isolated strains), while Klebsiella at 8.16% and Pseudomonas aeruginosa species at 5.63% were mainly emphasized in the case of Gram-negative bacilli. Two to seven pathogens were introduced after admission, suggesting that the community microbial environment is in a process of evolution and enrichment with hospital pathogens. Conclusions: The high level of positive bacteriological samples and the complex associations of the pathogens found at the admission bacteriological screening sustain the new idea that the pathogenic microorganisms existing in the community microbial environment have started to increasingly influence the hospital microbial environment, in contrast with the previous consideration, which emphasized only the unidirectional relationship between hospital infections and the changing bacteriological characteristics of the community environment. This modified paradigm must become the basis of a new personalized approach to the management of nosocomial infections.
The Role of Allotments and Community Gardens and the Challenges Facing Their Development in Urban Environments—A Literature Review
Authors: Kwartnik-Pruc Anita , Droj Gabriela
Published: January 2023

Current research largely focuses on the role of allotment gardens, the challenges facing them, and the direction of their future development in urban environments. The main idea behind the introduction of allotment gardens was to improve the living conditions and food supply of workers and the underprivileged. The impact of allotment gardening does not only concern the allotment gardeners but also the general public and the environment. It is important to emphasise that allotments have impacts not only on food production and outdoor physical activity, but also on the reuse of idle or neglected land, community development, therapeutic and nutritional benefits, and psychological benefits for allotment holders and residents. For this reason, this study captures six broad themes related to allotments: (1) community participation and cohesion, (2) health and well-being, (3) economic opportunities, (4) pollution, (5) urban planning and development, and (6) sustainable environment. The research is a systematic review in which steps were taken to minimise bias in the identification, selection, and summarisation of studies. The initial literature selection was based on a keyword search (title, abstract, and keywords) of the comprehensive literature databases Web of Science (all years) and Scopus (all years). The total scientific literature on which this review is based includes 162 research articles published between 1978 and July 2022. This work aims to fill these gaps and analyse existing knowledge by providing a detailed review of the academic literature, focusing not only on the benefits of community gardens and allotments in urban areas, but also on the existing problems related to allotments and urban gardening. Possible directions of development are also analysed based on the legal regulations in each country.
How Much Complexity Is Required for Modelling Grassland Production at Regional Scales?
Authors: Vogeler Iris , Kluß Christof , Peters Tammo , Taube Friedhelm
Published: January 2023

Studies evaluating the complexity of models, which are suitable to simulate grass growth at regional scales in intensive grassland production systems are scarce. Therefore, two different grass growth models (GrasProg1.0 and APSIM) with different complexity and input requirements were compared against long-term observations from variety trials with perennial ryegrass (Lolium perenne) in Germany and Denmark. The trial sites covered a large range of environmental conditions, with annual average temperatures ranging from 5.9 to 10.3 °C, and annual rainfall from 536 to 1154 mm. The sites also varied regarding soil type, which were for modelling categorised into three different groups according to their plant available water (PAW) content: light soils with a PAW of 60 mm, medium soils with a PAW of 80 mm, and heavy soils with a PAW of 100 mm. The objective was to investigate whether the simple model performed equally well with the given low number of inputs, namely climate and PAW group. Evaluation statistics showed that both models provided satisfactory results, with root mean square errors for individual cuts ranging from 0.59 to 1.28 t dry matter ha−1. The model efficiency (Nash–Sutcliffe efficiency) for the separate cuts were also good for both models, with 81% of the sites having a positive Nash–Sutcliffe efficiency value with GrasProg1.0, and 72% with APSIM. These results reveal that without detailed site-specific descriptions, the less complex GrasProg1.0 model can be incorporated into a simple decision support tool for optimising grassland management in intensive livestock production systems.
Local Observations of Climate Change and Adaptation Responses: A Case Study in the Mountain Region of Burundi-Rwanda
Authors: Nkurunziza Aline , Intwarinkase Mutaganzwa Dorine , Ndayitwayeko Willy Marcel , Nkengurutse Jacques , Kaplin Beth A. , Teixidor Toneu Irene , Zafra-Calvo Noelia , Cuni-Sanchez Aida
Published: January 2023

Mountain regions and their communities are particularly vulnerable to climate change impacts. However, little is known on the impacts observed and adaptation responses used in Burundi’s mountain region and if these are different to those reported in the contiguous mountain region of Rwanda. This paper aims to fill in these knowledge gaps. Semi-structured interviews were conducted with 300 smallholder farmers, 150 in northern Burundi and 150 in southern Rwanda. Farmers in both countries reported negative impacts on crops, animals, and human health, with small differences between countries driven by the main cultivated crops. More adaptation strategies were used in Burundi than in Rwanda, and more farmers in Burundi were using multiple strategies. In both countries, farmers’ wealth affected farmers’ adaptation responses and their food security. Notably, for all wealth groups (poor, average, rich), food security was lower in Rwanda than in Burundi. We relate our findings to current agricultural intensification policies in both countries and argue for the greater involvement of local farmers in adaptation planning using, for example, science-with-society approaches.
Regulation of Incentives for Use of Renewable Energy at the Level of Regional Legislation in Federal States, Using the Russian Federation as an Example
Authors: Kirichenko Anna , Kirichenko Ksenia , Kirichenko Evgeniy
Published: January 2023

Effective use of renewable energy requires a system of energy legislation that meets modern challenges. Although, in large countries, climate and socioeconomic factors in different regions can significantly vary and can affect the regional legislation regulating renewable energy sources, careful reproduction of good practices and successful experiences of other regions are a good basis for the development of legislation. The comparative method of legal research was the main method used to achieve the objectives set in this study. Based on the results, a number of recommendations were developed to consolidate and expand the powers of regional regulators in the field of renewable energy, to include an economic assessment of the effectiveness of state programs, to use tax incentives for renewable energy projects, and to introduce restrictions on the use of petroleum products. Recommendations were also made to improve regional legislation on renewable energy sources in terms of legal techniques. Further development of this study would contribute to the improvement of regional legal regulation and would accelerate the transition to “green” energy.
Low-Dose Blue Light (420 nm) Reduces Metabolic Activity and Inhibits Proliferation of Human Dermal Fibroblasts
Authors: Brüning Anne K. E. , Schiefer Jennifer L. , Fuchs Paul C. , Petzsch Patrick , Köhrer Karl , Suschek Christoph V. , Stürmer Ewa K. , Opländer Christian
Published: January 2023

Hypertrophic scarring in burn wounds is caused by overactive fibroblasts and myofibroblasts. Blue light reveals wavelength- and dose-dependent antibacterial and antiproliferative effects and may serve as a therapeutic option against wound infection and fibrotic conditions. Therefore, we evaluated in this study the effects of single and multiple irradiations with blue light at 420 nm (BL420) on the intracellular ATP concentration, and on the viability and proliferation of the human skin fibroblast (HDFs). In addition, possible BL420-induced effects on the catalase expression and differentiation were assessed by immunocytochemical staining and western blot analyses. Furthermore, we used RNA-seq analyses to identify BL420-affected genes. We found that BL420 induced toxicity in HDFs (up to 83%; 180 J/cm2). A low dose of 20 J/cm2 reduced the ATP concentration by ~50%. Multiple irradiations (4 × 20 J/cm2) inhibited proliferation without visible toxicity and reduced catalase protein expression by ~37% without affecting differentiation. The expression of about 300 genes was significantly altered. Many downregulated genes have functions in cell division/mitosis. BL420 can strongly influence the fibroblast physiology and has potential in wound therapy. However, it is important to consider the possible toxic and antiproliferative effects, which could potentially lead to impaired wound healing and reduced scar breaking strength.
Potential Health Benefits of Banana Phenolic Content during Ripening by Implementing Analytical and In Silico Techniques
Authors: Kritsi Eftichia , Tsiaka Thalia , Sotiroudis Georgios , Mouka Elizabeth , Aouant Konstantinos , Ladika Georgia , Zoumpoulakis Panagiotis , Cavouras Dionisis , Sinanoglou Vassilia J.
Published: January 2023

Banana ranks as the fifth most cultivated agricultural crop globally, highlighting its crucial socio-economic role. The banana’s health-promoting benefits are correlated with its composition in bioactive compounds, such as phenolic compounds. Thus, the present study attempts to evaluate the potential health benefits of banana phenolic content by combing analytical and in silico techniques. Particularly, the total phenolic content and antioxidant/antiradical activity of banana samples during ripening were determined spectrophotometrically. In parallel, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was implemented to unravel the variations in the phenolic profile of banana samples during ripening. Chlorogenic acid emerged as a ripening marker of banana, while apigenin and naringenin were abundant in the unripe fruit. In a further step, the binding potential of the elucidated phytochemicals was examined by utilizing molecular target prediction tools. Human carbonic anhydrase II (hCA-II) and XII (hCA-XII) enzymes were identified as the most promising targets and the inhibitory affinity of phenolic compounds was predicted through molecular docking studies. This class of enzymes is linked to a variety of pathological conditions, such as edema, obesity, hypertension, cancer, etc. The results assessment indicated that all assigned phenolic compounds constitute great candidates with potential inhibitory activity against CA enzymes.
Adaptive Interval Type-2 Fuzzy Neural Network Sliding Mode Control of Nonlinear Systems Using Improved Extended State Observer
Authors: Liu Lunhaojie , Fei Juntao , Yang Xianghua
Published: January 2023

An adaptive sliding mode control (ASMC) based on improved linear extended state observer (LESO) is proposed for nonlinear systems with unknown and uncertain dynamics. An improved LESO is designed to estimate total disturbance of the uncertain nonlinear system, and an interval type-2 fuzzy neural network (IT2FNN) is used to optimize and approximate the observe bandwidth of LESO, and the adaptive parameter tuning is realized based on the gradient descent (GD) method. Based on the total disturbance estimated by LESO, an ASMC strategy is designed to ensure the system stability. By adapting the sliding mode gain, the observation performance of LESO compared to the total disturbance can be better utilized, and system chattering is reduced. Finally, some simulation results are given which show that the proposed control strategy has a good control effect, strong practicability, and wide versatility.
A Parametric Method Optimised for the Solution of the (21)-Dimensional Nonlinear Schrödinger Equation+
Authors: Anastassi Zacharias A. , Kosti Athinoula A. , Rufai Mufutau Ajani
Published: January 2023

We investigate the numerical solution of the nonlinear Schrödinger equation in two spatial dimensions and one temporal dimension. We develop a parametric Runge–Kutta method with four of their coefficients considered as free parameters, and we provide the full process of constructing the method and the explicit formulas of all other coefficients. Consequently, we produce an adaptable method with four degrees of freedom, which permit further optimisation. In fact, with this methodology, we produce a family of methods, each of which can be tailored to a specific problem. We then optimise the new parametric method to obtain an optimal Runge–Kutta method that performs efficiently for the nonlinear Schrödinger equation. We perform a stability analysis, and utilise an exact dark soliton solution to measure the global error and mass error of the new method with and without the use of finite difference schemes for the spatial semi-discretisation. We also compare the efficiency of the new method and other numerical integrators, in terms of accuracy versus computational cost, revealing the superiority of the new method. The proposed methodology is general and can be applied to a variety of problems, without being limited to linear problems or problems with oscillatory/periodic solutions.
β-Lactams from the Ocean
Authors: Fisher Jed F. , Mobashery Shahriar
Published: January 2023

The title of this essay is as much a question as it is a statement. The discovery of the β-lactam antibiotics—including penicillins, cephalosporins, and carbapenems—as largely (if not exclusively) secondary metabolites of terrestrial fungi and bacteria, transformed modern medicine. The antibiotic β-lactams inactivate essential enzymes of bacterial cell-wall biosynthesis. Moreover, the ability of the β-lactams to function as enzyme inhibitors is of such great medical value, that inhibitors of the enzymes which degrade hydrolytically the β-lactams, the β-lactamases, have equal value. Given this privileged status for the β-lactam ring, it is therefore a disappointment that the exemplification of this ring in marine secondary metabolites is sparse. It may be that biologically active marine β-lactams are there, and simply have yet to be encountered. In this report, we posit a second explanation: that the value of the β-lactam to secure an ecological advantage in the marine environment might be compromised by its close structural similarity to the β-lactones of quorum sensing. The steric and reactivity similarities between the β-lactams and the β-lactones represent an outside-of-the-box opportunity for correlating new structures and new enzyme targets for the discovery of compelling biological activities.
Adherence to the Mediterranean Diet Association with Serum Inflammatory Factors Stress Oxidative and Appetite in COVID-19 Patients
Authors: Mohajeri Mahsa , Mohajery Reza , Cicero Arrigo F. G.
Published: January 2023

Background and Objectives: The Mediterranean diet’s bioactive components are suggested to strengthen the immune system and to exert anti-inflammatory actions. This study investigated the association between adherence to the Mediterranean diet with serum inflammatory factors, total antioxidant capacity, appetite, and symptoms of COVID-19 patients. Materials and Methods: This cross-sectional study was conducted among 600 Iranian COVID-19 patients selected by a simple random method. The ten-item Mediterranean diet adherence questionnaire was used to assess diet adherence. At the beginning of the study, 5 cc of blood was taken from all patients for measurement of serum interleukin 1β) IL-1β), tumor necrosis factor (TNF-α), malondialdehyde (MDA), high sensitivity C-reactive protein (hs-CRP) and total antioxidant capacity (TAC). A human ELISA kit with serial number 950.090.096 produced by the Diaclone Company was used to test this cytokine using the sandwich ELISA method. Results: One hundred and five patients presented a high adherence and 495 patients presented a low adherence to the Mediterranean diet. The incidence of fever, cough, diarrhea, taste changes, and pneumonia severity index were significantly lower in patients who adhered to the Mediterranean diet more than other patients. Serum levels of tumor necrosis factor (5.7 ± 2.1 vs. 6.9 ± 2.8 p = 0.02), interleukin 1 beta (3.2 ± 0.02 vs. 4.9 ± 0.01 p = 0.02), high-sensitivity C-reactive protein (17.08 ± 4.2 vs. 19.8 ± 2.5 p = 0.03), and malondialdehyde (5.7 ± 0.2 vs. 6.2 ± 0.3 p = 0.02) were significantly lower in patients who adhered more to the Mediterranean diet than other patients. Conclusion: The Mediterranean diet can improve the symptoms and elevated serum inflammatory factors in COVID-19 patients, so clinical trial studies are suggested to confirm this effect.
Novel Characterizations of Effective SIFs and Fatigue Crack Propagation Rate of Welded Rail Steel Using DIC
Authors: Fang Xiu-Yang , Gong Jian-En , Huang Wei , Wu Jia-Hong , Ding Jun-Jun
Published: January 2023

The fatigue crack growth-rate test of rail head, waist, and bottom material for U71Mn welded rail was carried out. Digital image correlation (DIC) was used to capture the full-field displacement. The crack-tip position was accurately obtained based on the full-field displacement data, and an accurate crack-tip opening displacement (CTOD) measurement point was found. The CTOD values of the welded rail head under overloaded and unloaded condition were extracted, and the area size of elastic CTOD and plastic CTOD was obtained. According to COD data under different experimental conditions, the corresponding crack opening force was extracted, the crack opening function introduced based on the Elber model, and a calculation method of effective stress-intensity factors (SIFs) considering the plasticity-induced crack closure proposed. The results in this paper provide some references for accurately assessing the fatigue life of welded rail.
Lipidomic Analysis of Cervicovaginal Fluid for Elucidating Prognostic Biomarkers and Relevant Phospholipid and Sphingolipid Pathways in Preterm Birth
Authors: Hong Se Hee , Lee Ji-Youn , Seo Sumin , Shin Bohyun , Jeong Cho Hee , Bae Eunbin , Kim Jiyu , Lee Donghee , An Byungchan , Shim Minki , Shin Jung Hoon , Lee Dong-Kyu , Kim Young Ju , Han Sang Beom
Published: January 2023

Cervicovaginal fluid (CVF) is an excellent specimen for monitoring preterm birth (PTB) as it characterizes cervical metabolites, the vaginal environment, and specific host immune responses. However, extensive lipid analysis of CVF to explain PTB has not been studied. In this study, we performed a systematic analysis combining high-throughput lipid analysis and omics to discover the unique metabolic properties of the cervix. Liquid chromatography-high resolution mass spectrometry successfully detected a total of 190 lipids in the CVF of 30 PTB and 30 term birth (TB) pregnant women. The whole lipidomics dataset analyzed by combining multivariate and univariate statistical analysis revealed 35 lipid biomarkers, including phospholipids and sphingolipids. Remarkably, sphingomyelin, which plays a physiologically essential role in sphingolipids, was significantly downregulated in PTB. Metabolic pathway study provides a close relationship between vaginal microbial organization and cell membrane formation, further supporting the robustness of our findings. Sphingolipids and phospholipids, which were determined to be important lipids for predicting PTB in our study, showed a high value of receiver operating characteristic (ROC) curve >0.7, indicating that a lipid diagnostic test and understanding the mechanism of lipids is highly related to the vaginal microbiome. Therefore, our result has high potential as a predictor of PTB.
Heterosigma akashiwo, a Fish-Killing Flagellate
Authors: Mehdizadeh Allaf Malihe
Published: January 2023

Heterosigma akashiwo is a golden-brown unicellular phytoflagellate with a high potential to create harmful algal blooms (HABs) and kill fish in many coastal regions worldwide, resulting in significant economic losses. Climate change and global warming have been introduced as triggers that impact the frequency and severity of H. akashiwo and other bloom-forming species in the past decades. In this review paper, the author tried to briefly discuss the morphology and taxonomy of H. akashiwo and show how environmental parameters can influence the physiology and toxicity of this species. Although the toxin production and mechanisms are still a conundrum, the proposed fish-killing mechanisms will be reviewed in the next step.
Core Genome Sequencing Analysis of E. coli O157:H7 Unravelling Genetic Relatedness among Strains from Cattle, Beef, and Humans in Bishoftu, Ethiopia
Authors: Gutema Fanta D. , De Zutter Lieven , Piérard Denis , Hinckel Bruno , Imamura Hideo , Rasschaert Geertrui , Abdi Reta D. , Agga Getahun E. , Crombé Florence
Published: January 2023

E. coli O157:H7 is a known Shiga toxin-producing Escherichia coli (STEC), causing foodborne disease globally. Cattle are the main reservoir and consumption of beef and beef products contaminated with E. coli O157:H7 is an important source of STEC infections in humans. To emphasize the cattle-to-human transmission through the consumption of contaminated beef in Bishoftu, Ethiopia, whole-genome sequencing (WGS) was performed on E. coli O157 strains isolated from three sources (cattle, beef, and humans). Forty-four E. coli O157:H7 isolates originating from 23 cattle rectal contents, three cattle hides, five beef carcasses, seven beef cuts at retail shops, and six human stools in Bishoftu between June 2017 and May 2019 were included. This study identified six clusters of closely related E. coli O157:H7 isolates based on core genome multilocus sequence typing (cgMLST) by targeting 2513 loci. A genetic linkage was observed among the isolate genomes from the cattle rectal contents, cattle hides, beef carcasses at slaughterhouses, beef at retail shops, and human stool within a time frame of 20 months. All the strains carried practically the same repertoire of virulence genes except for the stx2 gene, which was present in all but eight of the closely related isolates. All the strains carried the mdfA gene, encoding for the MdfA multi-drug efflux pump. CgMLST analysis revealed genetically linked E. coli O157:H7 isolates circulating in the area, with a potential transmission from cattle to humans through the consumption of contaminated beef and beef products.
Adaptation of Lacticaseibacillus rhamnosus CM MSU 529 to Aerobic Growth: A Proteomic Approach
Authors: Dinarieva Tatiana Yu. , Klimko Alena I. , Kahnt Jörg , Cherdyntseva Tatiana A. , Netrusov Alexander I.
Published: January 2023

The study describes the effect of aerobic conditions on the proteome of homofermentative lactic acid bacterium Lacticaseibacillus rhamnosus CM MSU 529 grown in a batch culture. Aeration caused the induction of the biosynthesis of 43 proteins, while 14 proteins were downregulated as detected by label-free LC-MS/MS. Upregulated proteins are involved in oxygen consumption (Pox, LctO, pyridoxine 5’-phosphate oxidase), xylulose 5-phosphate conversion (Xfp), pyruvate metabolism (PdhD, AlsS, AlsD), reactive oxygen species (ROS) elimination (Tpx, TrxA, Npr), general stress response (GroES, PfpI, universal stress protein, YqiG), antioxidant production (CysK, DkgA), pyrimidine metabolism (CarA, CarB, PyrE, PyrC, PyrB, PyrR), oligopeptide transport and metabolism (OppA, PepO), and maturation and stability of ribosomal subunits (RbfA, VicX). Downregulated proteins participate in ROS defense (AhpC), citrate and pyruvate consumption (CitE, PflB), oxaloacetate production (AvtA), arginine synthesis (ArgG), amino acid transport (GlnQ), and deoxynucleoside biosynthesis (RtpR). The data obtained shed light on mechanisms providing O2-tolerance and adaptation to aerobic conditions in strain CM MSU 529. The biosynthesis of 39 from 57 differentially abundant proteins was shown to be O2-sensitive in lactic acid bacteria for the first time. To our knowledge this is the first study on the impact of aerobic cultivation on the proteome of L. rhamnosus.
Natural Flavonoid Derivatives Have Pan-Coronavirus Antiviral Activity
Authors: Mori Mattia , Quaglio Deborah , Calcaterra Andrea , Ghirga Francesca , Sorrentino Leonardo , Cammarone Silvia , Fracella Matteo , D’Auria Alessandra , Frasca Federica , Criscuolo Elena , Clementi Nicola , Mancini Nicasio , Botta Bruno , Antonelli Guido , Pierangeli Alessandra , Scagnolari Carolina
Published: January 2023

The SARS-CoV-2 protease (3CLpro) is one of the key targets for the development of efficacious drugs for COVID-19 treatment due to its essential role in the life cycle of the virus and exhibits high conservation among coronaviruses. Recent studies have shown that flavonoids, which are small natural molecules, have antiviral activity against coronaviruses (CoVs), including SARS-CoV-2. In this study, we identified the docking sites and binding affinity of several natural compounds, similar to flavonoids, and investigated their inhibitory activity towards 3CLpro enzymatic activity. The selected compounds were then tested in vitro for their cytotoxicity, for antiviral activity against SARS-CoV-2, and the replication of other coronaviruses in different cell lines. Our results showed that Baicalein (100 μg/mL) exerted strong 3CLpro activity inhibition (>90%), whereas Hispidulin and Morin displayed partial inhibition. Moreover, Baicalein, up to 25 μg/mL, hindered >50% of SARS-CoV-2 replication in Vero E6 cultures. Lastly, Baicalein displayed antiviral activity against alphacoronavirus (Feline-CoV) and betacoronavirus (Bovine-CoV and HCoV-OC43) in the cell lines. Our study confirmed the antiviral activity of Baicalein against SARS-CoV-2 and demonstrated clear evidence of its pan-coronaviral activity.
ZIF-67-Derived NiCo-Layered Double Hydroxide@Carbon Nanotube Architectures with Hollow Nanocage Structures as Enhanced Electrocatalysts for Ethanol Oxidation Reaction
Authors: Li Yixuan , Xu Yanqi , Li Cunjun , Zhu Wenfeng , Chen Wei , Zhao Yufei , Liu Ruping , Wang Linjiang
Published: January 2023

The rational design of efficient Earth-abundant electrocatalysts for the ethanol oxidation reaction (EOR) is the key to developing direct ethanol fuel cells (DEFCs). Among these, the smart structure is highly demanded for highly efficient and stable non-precious electrocatalysts based on transition metals (such as Ni, Co, and Fe). In this work, high-performance NiCo-layered double hydroxide@carbon nanotube (NiCo-LDH@CNT) architectures with hollow nanocage structures as electrocatalysts for EOR were prepared via sacrificial ZIF-67 templates on CNTs. Comprehensive structural characterizations revealed that the as-synthesized NiCo-LDH@CNTs architecture displayed 3D hollow nanocages of NiCo-LDH and abundant interfacial structure between NiCo-LDH and CNTs, which could not only completely expose active sites by increasing the surface area but also facilitate the electron transfer during the electrocatalytic process, thus, improving EOR activity. Benefiting from the 3D hollow nanocages and interfacial structure fabricated by the sacrificial ZIF-67-templated method, the NiCo-LDH@CNTs-2.5% architecture exhibited enhanced electrocatalytic activity for ethanol oxidation compared to single-component NiCo-LDH, where the peak current density was 11.5 mA·cm−2, and the jf/jb value representing the resistance to catalyst poisoning was 1.72 in an alkaline environment. These results provide a new perspective on the fabrication of non-precious metal electrocatalysts for EOR in DEFCs.
Synthesis, Characterization, and Study of Catalytic Activity of Chiral Cu(II) and Ni(II) Salen Complexes in the α-Amino Acid C-α Alkylation Reaction
Authors: Tovmasyan Anna S. , Mkrtchyan Anna F. , Khachatryan Hamlet N. , Hayrapetyan Mary V. , Hakobyan Robert M. , Poghosyan Artavazd S. , Tsaturyan Avetis H. , Minasyan Ela V. , Maleev Victor I. , Larionov Vladimir A. , Ayvazyan Armen G. , Shibata Norio , Roviello Giovanni N. , Saghyan Ashot S.
Published: January 2023

A new family of Cu(II) and Ni(II) salen complexes was synthesized and fully characterized through various physicochemical methods. Their catalytic activity was evaluated in the phase transfer Cα-alkylation reaction of the Schiff bases of D,L-alanine ester and benzaldehyde derivatives. It was found that the introduction of a chlorine atom into the ortho- and para-positions of the phenyl ring of the substrate resulted in an increase in both the chemical yield and the asymmetric induction (ee 66–98%). The highest enantiomeric excess was achieved in the case of a Cu(II) salen complex based on (S,S)-cyclohexanediamine and salicylaldehyde at −20 °C. The occurrence of a bulky substituent in the ligand present in the complexes led to a drastic decrease in ee and chemical yield. For instance, the introduction of bulky substituents at positions 3 and 5 of the phenyl ring of the catalyst resulted in a complete loss of the stereoselectivity control in the alkylation reaction.