bluuebunny/crossref_metadata_2025 huggingface dataset:
Promoters versus Adversaries of Change: Agent-Based Modeling of Organizational Conflict in Co-Evolving Networks
Authors: Ioannidis Evangelos , Varsakelis Nikos , Antoniou Ioannis
Published: December 2020

The social adoption of change is usually hard because in reality, forces opposing the social adoption of change manifest. This situation of organizational conflict corresponds to the case where two competing groups of influential agents (“promoters” versus “adversaries” of change) operate concurrently within the same organizational network. We model and explore the co-evolution of interpersonal ties and attitudes in the presence of conflict, taking into account explicitly the microscopic “agent-to-agent” interactions. In this perspective, we propose a new ties-attitudes co-evolution model where the diffusion of attitudes depends on the weights and the evolution of weights is formulated as a “learning mechanism” (weight updates depend on the previous values of both weights and attitudes). As a result, the co-evolution is intrinsic/endogenous. We simulate representative scenarios of conflict in 4 real organizational networks. In order to formulate structural balance in directed networks, we extended Heider’s definition of balance considering directed triangles. The evolution of balance involves two stages: first, negative links pop up disorderly and destroy balance, but after some time, as new negative links are formed, a “new” balance is re-established. This “new” balance is emerging concurrently with the polarization of attitudes or domination of one attitude. Moreover, same-minded agents are positively linked and different-minded agents are negatively-linked. This macroscopic self-organization of the system is due only to agent-to-agent interactions, involving feedbacks on weight updates at the local microscopic level.
Essential Oils as Alternative Biocides for the Preservation of Waterlogged Archaeological Wood
Authors: Antonelli Federica , Bartolini Marco , Plissonnier Marie-Laure , Esposito Alfonso , Galotta Giulia , Ricci Sandra , Davidde Petriaggi Barbara , Pedone Cristian , Di Giovanni Antonella , Piazza Silvano , Guerrieri Francesca , Romagnoli Manuela
Published: December 2020

Waterlogged archaeological wood is exposed to a high risk of biological degradation during the post-excavation phases of storage and restoration. For this reason, often biocides must be used to preserve wooden remains. In the present work three essential oils (cinnamon, wild thyme, and common thyme) were tested as possible alternative biocides to use in the preservation of waterlogged archaeological wood. The oils were first tested in vitro to establish the minimum inhibitory concentration (MIC) and to evaluate the biocidal activity on selected fungal strains. Then, the established MIC was applied on waterlogged archaeological wood samples and during an actual restoration treatment. The effectiveness of the oils was evaluated through cultural analyses, ATP quantification, and next-generation sequencing. The results showed that the oils caused a significant decrease in the vitality of fungal mycelia grown in vitro and of the microbiota present in treated wood and storage water. Furthermore, an influence on the composition of the bacterial communities of treated wood samples was observed. Although further tests are needed to evaluate interferences with the materials used during restoration procedures, essential oils could be considered as a possible alternative to the currently used biocide.
Eukaryotic and Prokaryotic Microbiota Interactions
Authors: Kodio Aly , Menu Estelle , Ranque Stéphane
Published: December 2020

The nature of the relationship between the communities of microorganisms making up the microbiota in and on a host body has been increasingly explored in recent years. Microorganisms, including bacteria, archaea, viruses, parasites and fungi, have often long co-evolved with their hosts. In human, the structure and diversity of microbiota vary according to the host’s immunity, diet, environment, age, physiological and metabolic status, medical practices (e.g., antibiotic treatment), climate, season and host genetics. The recent advent of next generation sequencing (NGS) technologies enhanced observational capacities and allowed for a better understanding of the relationship between distinct microorganisms within microbiota. The interaction between the host and their microbiota has become a field of research into microorganisms with therapeutic and preventive interest for public health applications. This review aims at assessing the current knowledge on interactions between prokaryotic and eukaryotic communities. After a brief description of the metagenomic methods used in the studies were analysed, we summarise the findings of available publications describing the interaction between the bacterial communities and protozoa, helminths and fungi, either in vitro, in experimental models, or in humans. Overall, we observed the existence of a beneficial effect in situations where some microorganisms can improve the health status of the host, while the presence of other microorganisms has been associated with pathologies, resulting in an adverse effect on human health.
The Role of Petrimonas mucosa ING2-E5AT in Mesophilic Biogas Reactor Systems as Deduced from Multiomics Analyses
Authors: Maus Irena , Tubbesing Tom , Wibberg Daniel , Heyer Robert , Hassa Julia , Tomazetto Geizecler , Huang Liren , Bunk Boyke , Spröer Cathrin , Benndorf Dirk , Zverlov Vladimir , Pühler Alfred , Klocke Michael , Sczyrba Alexander , Schlüter Andreas
Published: December 2020

Members of the genera Proteiniphilum and Petrimonas were speculated to represent indicators reflecting process instability within anaerobic digestion (AD) microbiomes. Therefore, Petrimonas mucosa ING2-E5AT was isolated from a biogas reactor sample and sequenced on the PacBio RSII and Illumina MiSeq sequencers. Phylogenetic classification positioned the strain ING2-E5AT in close proximity to Fermentimonas and Proteiniphilum species (family Dysgonomonadaceae). ING2-E5AT encodes a number of genes for glycosyl-hydrolyses (GH) which are organized in Polysaccharide Utilization Loci (PUL) comprising tandem susCD-like genes for a TonB-dependent outer-membrane transporter and a cell surface glycan-binding protein. Different GHs encoded in PUL are involved in pectin degradation, reflecting a pronounced specialization of the ING2-E5AT PUL systems regarding the decomposition of this polysaccharide. Genes encoding enzymes participating in amino acids fermentation were also identified. Fragment recruitments with the ING2-E5AT genome as a template and publicly available metagenomes of AD microbiomes revealed that Petrimonas species are present in 146 out of 257 datasets supporting their importance in AD microbiomes. Metatranscriptome analyses of AD microbiomes uncovered active sugar and amino acid fermentation pathways for Petrimonas species. Likewise, screening of metaproteome datasets demonstrated expression of the Petrimonas PUL-specific component SusC providing further evidence that PUL play a central role for the lifestyle of Petrimonas species.
A Column Leaching Model of Low-Grade Chalcopyrite Ore: Mineral Preferences and Chemical Reactivity
Authors: Bostelmann Heike , Southam Gordon
Published: December 2020

Bioleaching models to examine copper extraction from low-grade chalcopyrite ores were set up to identify the influence of pyrite on leaching efficacy. A combination of scanning electron microscopy and geochemical analysis showed that extraction was marginally enhanced by the addition of pyrite when using a combination of Leptospirillum ferrooxidans, an iron oxidiser, Acidithiobacillus thiooxidans, a sulphur oxidising species and Acidithiobacillus ferrooxidans, an iron and sulphur oxidiser. Extensive biofilms formed on the pyrite surfaces (>106 cells/mm2) but were severely limited on chalcopyrite, possessing approximately the same number of cells as quartz grains, an internal non-nutrient control “substrate” (with ca. 2 × 103 cells/mm2). The presence of dissolved copper did not inhibit the growth of this consortium. Indirect “bioleaching” of chalcopyrite appears to be limited by proton activity at the chalcopyrite surface.
Transition of Sulphide Self-Heating from Stage A to Stage B
Authors: Moon Sungjae , Rosenblum Frank , Tan Yuehua , Waters Kristian E. , Finch James A.
Published: December 2020

Previous work has shown that sulphide self-heating occurs in three distinct stages, referred to as Stage A, Stage B and Stage C. In this publication, the focus is the transition from Stage A to Stage B which occurs at ca. 100 °C. Background literature hints that the transition corresponds to the transformation of the rhombic form of elemental sulphur to the more reactive monoclinic form that occurs at 96 °C. A test apparatus is modified for adiabatic heating to track the transition. The results support this transformation of sulphur as being key to the transition, and the transition temperature is thus modified to 96 °C. Variations in a sample’s response under adiabatic conditions are observed and possible reasons are discussed. Testing in adiabatic mode provides new insights into the sulphide self-heating process that complements the test designed to identify propensity to self-heat.
Enhancing Ion Flotation through Decoupling the Overflow Gas and Liquid Fluxes
Authors: Baynham Siân , Ireland Peter , Galvin Kevin
Published: December 2020

Conventional ion flotation is hydrodynamically constrained by coupling of the gas flux and liquid flux that report to the concentrate. This constraint has greatly limited the industrial application of ion flotation, despite its remarkable effectiveness in extracting ionic species down to very low concentrations, of order 1 ppm. Previous work demonstrated that these hydrodynamic constraints could be significantly relaxed using the reflux flotation cell (RFC), a system incorporating parallel inclined channels to improve bubble-liquid segregation. However, it was found that bubble coalescence placed an additional limit on performance. In this study the impact of coalescence was minimized by reducing the volume reduction from 20 to 5, ensuring sufficient liquid reported to the concentrate with the bubbles. Under these conditions, an equivalent adsorptive recovery was achieved using the RFC at feed fluxes up to four times those in the conventional system. The maximum adsorptive extraction rate achieved with the RFC was three times that for the conventional system. A refined experimental methodology was used to quantify much more accurately the relative hydrodynamic limits of conventional and RFC operation. The previously neglected issue of split-zone segregation, resulting in smaller bubbles in the lower part of the cell, was also investigated.
Nano-Biomimetic Drug Delivery Vehicles: Potential Approaches for COVID-19 Treatment
Authors: Witika Bwalya A. , Makoni Pedzisai A. , Mweetwa Larry L. , Ntemi Pascal V. , Chikukwa Melissa T. R. , Matafwali Scott K. , Mwila Chiluba , Mudenda Steward , Katandula Jonathan , Walker Roderick B.
Published: December 2020

The current COVID-19 pandemic has tested the resolve of the global community with more than 35 million infections worldwide and numbers increasing with no cure or vaccine available to date. Nanomedicines have an advantage of providing enhanced permeability and retention and have been extensively studied as targeted drug delivery strategies for the treatment of different disease. The role of monocytes, erythrocytes, thrombocytes, and macrophages in diseases, including infectious and inflammatory diseases, cancer, and atherosclerosis, are better understood and have resulted in improved strategies for targeting and in some instances mimicking these cell types to improve therapeutic outcomes. Consequently, these primary cell types can be exploited for the purposes of serving as a “Trojan horse” for targeted delivery to identified organs and sites of inflammation. State of the art and potential utilization of nanocarriers such as nanospheres/nanocapsules, nanocrystals, liposomes, solid lipid nanoparticles/nano-structured lipid carriers, dendrimers, and nanosponges for biomimicry and/or targeted delivery of bioactives to cells are reported herein and their potential use in the treatment of COVID-19 infections discussed. Physicochemical properties, viz., hydrophilicity, particle shape, surface charge, composition, concentration, the use of different target-specific ligands on the surface of carriers, and the impact on carrier efficacy and specificity are also discussed.
Development of Advanced Chemometric-Assisted Spectrophotometric Methods for the Determination of Cromolyn Sodium and Its Alkaline Degradation Products
Authors: El Zahar Noha M. , Tadros Mariam M. , Ayoub Bassam M.
Published: December 2020

Advanced and sensitive spectrophotometric and chemometric analytical methods were successfully established for the stability-indicating assay of cromolyn sodium (CS) and its alkaline degradation products (Deg1 and Deg2). Spectrophotometric mean centering ratio spectra method (MCR) and chemometric methods, including principal component regression (PCR) and partial least square (PLS-2) methods, were applied. Peak amplitudes after MCR at 367.8 nm, 373.8 nm and 310.6 nm were used within linear concentration ranges of 2–40 µg mL−1, 5–40 µg mL−1 and 10–100 µg mL−1 for CS, Deg1 and Deg2, respectively. For PCR and PLS-2 models, a calibration set of eighteen mixtures and a validation set of seven mixtures were built for the simultaneous determination of CS, Deg1 and Deg2 in the ranges of 5–13 µg mL−1, 8–16 µg mL−1, and 10–30 µg mL−1, respectively. The authors emphasize the importance of a stability-indicating strategy for the investigation of pharmaceutical products.
Unripe Rubus coreanus Miquel Extract Containing Ellagic Acid Promotes Lipolysis and Thermogenesis In Vitro and In Vivo
Authors: Kim Kyeong Jo , Jeong Eui-Seon , Lee Ki Hoon , Na Ju-Ryun , Park Soyi , Kim Jin Seok , Na Chang-Su , Kim Young Ran , Kim Sunoh
Published: December 2020

Previously, we demonstrated that a 5% ethanol extract of unripe Rubus coreanus (5-uRCK) and ellagic acid has hypocholesterolemic and antiobesity activity, at least partially mediated by the downregulation of adipogenic and lipogenic gene expression in high-fat diet (HFD)-fed animals. The present study investigated the thermogenic and lipolytic antiobesity effects of 5-uRCK and ellagic acid in HFD-induced obese C57BL/6 mice and explored its mechanism of action. Mice fed an HFD received 5-uRCK or ellagic acid as a post-treatment or pretreatment. Both post-treated and pretreated mice showed significant reductions in body weight and adipose tissue mass compared to the HFD-fed mice. The protein levels of lipolysis-associated proteins, such as adipose triglyceride lipase (ATGL), phosphorylated hormone-sensitive lipase (p-HSL), and perilipin1 (PLIN1), were significantly increased in both the 5-uRCK- and ellagic acid-treated mouse epididymal white adipose tissue (eWAT). Additionally, thermogenesis-associated proteins, such as peroxisome proliferator-activated receptor α (PPARα), carnitine palmitoyl transferase-1 (CPT1), uncoupling protein 1 (UCP1), and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC1α), in inguinal white adipose tissue (ingWAT) were clearly increased in both the 5-uRCK- and ellagic acid-treated mice compared to HFD-fed mice. These results suggest that 5-uRCK and ellagic acid are effective for suppressing body weight gain and enhancing the lipid profile.
Recent Progress in Small Spirocyclic, Xanthene-Based Fluorescent Probes
Authors: G. Keller Sascha , Kamiya Mako , Urano Yasuteru
Published: December 2020

The use of fluorescent probes in a multitude of applications is still an expanding field. This review covers the recent progress made in small molecular, spirocyclic xanthene-based probes containing different heteroatoms (e.g., oxygen, silicon, carbon) in position 10′. After a short introduction, we will focus on applications like the interaction of probes with enzymes and targeted labeling of organelles and proteins, detection of small molecules, as well as their use in therapeutics or diagnostics and super-resolution microscopy. Furthermore, the last part will summarize recent advances in the synthesis and understanding of their structure–behavior relationship including novel computational approaches.
Oligosaccharides: Defense Inducers, Their Recognition in Plants, Commercial Uses and Perspectives
Authors: Guarnizo Nathalie , Oliveros Diego , Murillo-Arango Walter , Bermúdez-Cardona María Bianney
Published: December 2020

Plants have innate immune systems or defense mechanisms that respond to the attack of pathogenic microorganisms. Unlike mammals, they lack mobile defense cells, so defense processes depend on autonomous cellular events with a broad repertoire of recognition to detect pathogens, which compensates for the lack of an adaptive immune system. These defense mechanisms remain inactive or latent until they are activated after exposure or contact with inducing agents, or after the application of the inductor; they remain inactive only until they are affected by a pathogen or challenged by an elicitor from the same. Resistance induction represents a focus of interest, as it promotes the activation of plant defense mechanisms, reducing the use of chemical synthesis pesticides, an alternative that has even led to the generation of new commercial products with high efficiency, stability and lower environmental impact, which increase productivity by reducing not only losses but also increasing plant growth. Considering the above, the objective of this review is to address the issue of resistance induction with a focus on the potential of the use of oligosaccharides in agriculture, how they are recognized by plants, how they can be used for commercial products and perspectives.
Silicon Nanotubes Fabricated by Wet Chemical Etching of ZnO/Si Core–Shell Nanowires
Authors: Sun Yong-Lie , Zheng Xiang-Dong , Jevasuwan Wipakorn , Fukata Naoki
Published: December 2020

Silicon nanotubes (SiNTs) have garnered a great deal of interest for both their synthesis and their potential for application to high-capacity energy storage, biosensors, and selective transport. In this study, we report a convenient and low-cost route to the fabrication of vertically aligned SiNTs via a wet-etching process that enables the control of the wall thickness of SiNTs by varying the gas flux and growth temperature. Transmission electron microscopy (TEM) characterization showed the resultant SiNTs to have an amorphous nature and a hexagonal hollow core. These SiNTs can be crystallized by thermal annealing.
In-Operando Diffraction and Spectroscopic Evaluation of Pure, Zr-, and Ce-Doped Vanadium Dioxide Thermochromic Films Derived via Glycolate Synthesis
Authors: Kurajica Stanislav , Mandić Vilko , Panžić Ivana , Gaboardi Mattia , Mužina Katarina , Lozančić Ana , Šipušić Juraj , Munda Ivana Katarina , Višić Lucija , Blagojević Sanja Lučić , Gigli Lara , Plaisier Jasper Rikkert
Published: December 2020

Pure and doped vanadia (VO2, V0.98Zr0.02O2, V0.98Ce0.02O2) samples were prepared by wet chemistry synthesis from vanadyl glycolate intermediate phase and tape casted into films. Combining in-operando grazing incidence synchrotron X-ray diffraction and Raman spectroscopy, we studied the structural evolution of the films under isothermal conditions. The setup allowed assessment of the thermochromic functionality with continuous monitoring of the monoclinic to tetragonal transition in pure and doped vanadia phases, responsible for the transmission and reflection of light in the infrared part of the solar spectrum. The materials characterisation by X-ray diffraction beamline (MCX) goniometer demonstrated ideal performance, combining flexible geometry, high resolution, and the potential to accommodate the multi-channel equipment for in-operando characterisation. This method proved viable for evaluating the relevant structural and physical, and thereof functional properties of these systems. We revealed that dopants reduce the transition temperature by 5 °C on average. The synthetic route of the films was held responsible for the observed phase separation. The more favourable behaviour of cerium-doped sample was attributed to cerium alkoxide behaviour. In addition, structural, microstructural, thermal, and spectroscopic characterisation on powder samples was performed to gain more insight into the development of the phases that are responsible for thermochromic features in a broader range of doping ratios. The influence of the dopants on the extent of the thermochromic transition (transmission to reflection hysteresis) was also evaluated using (micro) structural, thermal and spectroscopic methods of powder samples. Characterisations showed that zirconium doping in 2, 4, and 6 mol% significantly influenced the phase composition and morphology of the precursor. Vanadium oxides other than VO2 can easily crystallise; however, a thermal treatment regime that allowed crystallisation of VO2 as a single phase was established.
Solid-State Dewetting Dynamics of Amorphous Ge Thin Films on Silicon Dioxide Substrates
Authors: Toliopoulos Dimosthenis , Fedorov Alexey , Bietti Sergio , Bollani Monica , Bonera Emiliano , Ballabio Andrea , Isella Giovanni , Bouabdellaoui Mohammed , Abbarchi Marco , Tsukamoto Shiro , Sanguinetti Stefano
Published: December 2020

We report on the dewetting process, in a high vacuum environment, of amorphous Ge thin films on SiO2/Si (001). A detailed insight of the dewetting is obtained by in situ reflection high-energy electron diffraction and ex situ scanning electron microscopy. These characterizations show that the amorphous Ge films dewet into Ge crystalline nano-islands with dynamics dominated by crystallization of the amorphous material into crystalline nano-seeds and material transport at Ge islands. Surface energy minimization determines the dewetting process of crystalline Ge and controls the final stages of the process. At very high temperatures, coarsening of the island size distribution is observed.
Caffeine-Containing Energy Shots Cause Acute Impaired Glucoregulation in Adolescents
Authors: Shearer Jane , Reimer Raylene A. , Hittel Dustin S. , Gault Mackenzie A. , Vogel Hans J. , Klein Matthias S.
Published: December 2020

Caffeine-containing, nutritionally fortified energy shots are consumed at high rates by adolescents, yet little is known about their metabolic impact. The purpose of this study was to examine the consequences of small format, caffeinated energy shots on glucose metabolism and gastrointestinal hormone secretion in adolescents. Twenty participants aged 13–19 years participated in a double-blind, randomized cross-over study consisting of two trials separated by 1–4 weeks. Participants consumed a volume-matched caffeinated energy shot (CAF, 5 mg/kg) or a decaffeinated energy shot (DECAF) followed by a 2 h oral glucose tolerance test. Blood samples were collected and area under the curve (AUC) calculated for glucose, insulin and gut and metabolic hormones. Consumption of CAF resulted in a 25% increase in glucose and a 26% increase in insulin area under the curve (AUC, p = 0.037; p < 0.0001) compared to DECAF. No impact on gut hormones was observed. To further characterize responses, individuals were classified as either slow or fast caffeine metabolizers based on an allele score. Glucose intolerance was greater in genetically fast vs. slow caffeine metabolizers and differences between groups were supported by distinct serum metabolomics separation. Consumption of caffeine-containing energy shots results in acute impaired glucoregulation in healthy adolescents as characterized by hyperinsulinemia following an oral glucose challenge.
Milk Containing A2 β-Casein ONLY, as a Single Meal, Causes Fewer Symptoms of Lactose Intolerance than Milk Containing A1 and A2 β-Caseins in Subjects with Lactose Maldigestion and Intolerance: A Randomized, Double-Blind, Crossover Trial
Authors: Ramakrishnan Monica , Eaton Tracy K. , Sermet Omer M. , Savaiano Dennis A.
Published: December 2020

Acute-feeding and multiple-day studies have demonstrated that milk containing A2 β-casein only causes fewer symptoms of lactose intolerance (LI) than milk containing both A1 and A2 β-caseins. We conducted a single-meal study to evaluate the gastrointestinal (GI) tolerance of milk containing different concentrations of A1 and A2 β-casein proteins. This was a randomized, double-blind, crossover trial in 25 LI subjects with maldigestion and an additional eight lactose maldigesters who did not meet the QLCSS criteria. Subjects received each of four types of milk (milk containing A2 β-casein protein only, Jersey milk, conventional milk, and lactose-free milk) after overnight fasting. Symptoms of GI intolerance and breath hydrogen concentrations were analyzed for 6 h after ingestion of each type of milk. In an analysis of the 25 LI subjects, total symptom score for abdominal pain was lower following consumption of milk containing A2 β-casein only, compared with conventional milk (p = 0.004). Post hoc analysis with lactose maldigesters revealed statistically significantly improved symptom scores (p = 0.04) and lower hydrogen production (p = 0.04) following consumption of milk containing A2 β-casein only compared with conventional milk. Consumption of milk containing A2 β-casein only is associated with fewer GI symptoms than consumption of conventional milk in lactose maldigesters.
Midwifery Education Institutions in Italy Creation and Validation of Clinical Preceptors’ Assessment Tool: Students’ and Expert Midwives’ Views
Authors: Mauri Paola Agnese , Cortinovis Ivan , Guerrini Contini Norma Nilde , Soldi Marta
Published: December 2020

Background: The aim of the study is to create and validate a midwifery preceptor’s evaluation form to be used by midwifery students. The International Confederation of Midwives recommends that clinical placements need to be supervised by a preceptor in order to be efficient for students who, in this way, gain competence and proper practice within the midwifery practical area. Methods: This is an observational multi-center transversal study and leads to the validation of an evaluation questionnaire. Methodically, the following steps were followed: literature review, focus group with midwifery students, meeting between expert midwives, creation of the preceptor’s assessment form, filling in of the forms by midwifery students and expert midwives, and validation of the form. The study was carried out in eight Italian universities and included eighty-eight midwifery students and eight midwives. Results and Conclusion: A midwifery preceptor’s assessment questionnaire was created made up of four attribute areas which, as a total, included 33 items. Cronbach’s alpha score was calculated after examining the forms filled in by students and expert midwives. An alpha score of 0.97–0.85 was obtained. The result was Pearson Correlation Coefficient r = 0.78.
Differential Mortality and High Viral Load in Naive Pacific Oyster Families Exposed to OsHV-1 Suggests Tolerance Rather than Resistance to Infection
Authors: Agnew M. Victoria , Friedman Carolyn S. , Langdon Christopher , Divilov Konstantin , Schoolfield Blaine , Morga Benjamin , Degremont Lionel , Dhar Arun K. , Kirkland Peter , Dumbauld Brett , Burge Colleen A.
Published: December 2020

Pacific oysters, Crassostrea gigas, are one of the most productive aquaculture species in the world. However, they are threatened by the spread of Ostreid herpesvirus-1 (OsHV-1) and its microvariants (collectively “µvars”), which cause mass mortalities in all life stages of Pacific oysters globally. Breeding programs have been successful in reducing mortality due to OsHV-1 variants following viral outbreaks; however, an OsHV-1-resistant oyster line does not yet exist in the United States (US), and it is unknown how OsHV-1 µvars will affect US oyster populations compared to the current variant, which is similar to the OsHV-1 reference, found in Tomales Bay, CA. The goals of this study were to investigate the resistance of C. gigas juveniles produced by the Molluscan Broodstock Program (MBP) to three variants of OsHV-1: a California reference OsHV-1, an Australian µvar, and a French µvar. This is the first study to directly compare OsHV-1 µvars to a non-µvar. The survival probability of oysters exposed to the French (FRA) or Australian (AUS) µvar was significantly lower (43% and 71%, respectively) than to the reference variant and controls (96%). No oyster family demonstrated resistance to all three OsHV-1 variants, and many surviving oysters contained high copy numbers of viral DNA (mean ~3.53 × 108). These results indicate that the introduction of OsHV-1 µvars could have substantial effects on US Pacific oyster aquaculture if truly resistant lines are not achieved, and highlight the need to consider resistance to infection in addition to survival as traits in breeding programs to reduce the risk of the spread of OsHV-1 variants.
Conjugates Containing Two and Three Trithiolato-Bridged Dinuclear Ruthenium(II)-Arene Units as In Vitro Antiparasitic and Anticancer Agents
Authors: Studer Valentin , Anghel Nicoleta , Desiatkina Oksana , Felder Timo , Boubaker Ghalia , Amdouni Yosra , Ramseier Jessica , Hungerbühler Martin , Kempf Christoph , Heverhagen Johannes Thomas , Hemphill Andrew , Ruprecht Nico , Furrer Julien , Păunescu Emilia
Published: December 2020

The synthesis, characterization, and in vitro antiparasitic and anticancer activity evaluation of new conjugates containing two and three dinuclear trithiolato-bridged ruthenium(II)-arene units are presented. Antiparasitic activity was evaluated using transgenic Toxoplasmagondii tachyzoites constitutively expressing β-galactosidase grown in human foreskin fibroblasts (HFF). The compounds inhibited T.gondii proliferation with IC50 values ranging from 90 to 539 nM, and seven derivatives displayed IC50 values lower than the reference compound pyrimethamine, which is currently used for treatment of toxoplasmosis. Overall, compound flexibility and size impacted on the anti-Toxoplasma activity. The anticancer activity of 14 compounds was assessed against cancer cell lines A2780, A2780cisR (human ovarian cisplatin sensitive and resistant), A24, (D-)A24cisPt8.0 (human lung adenocarcinoma cells wild type and cisPt resistant subline). The compounds displayed IC50 values ranging from 23 to 650 nM. In A2780cisR, A24 and (D-)A24cisPt8.0 cells, all compounds were considerably more cytotoxic than cisplatin, with IC50 values lower by two orders of magnitude. Irrespective of the nature of the connectors (alkyl/aryl) or the numbers of the di-ruthenium units (two/three), ester conjugates 6–10 and 20 exhibited similar antiproliferative profiles, and were more cytotoxic than amide analogues 11–14, 23, and 24. Polynuclear conjugates with multiple trithiolato-bridged di-ruthenium(II)-arene moieties deserve further investigation.
Engineering of Doxorubicin-Encapsulating and TRAIL-Conjugated Poly(RGD) Proteinoid Nanocapsules for Drug Delivery Applications
Authors: Hadad Elad , Rudnick-Glick Safra , Itzhaki Ella , Avivi Matan Y. , Grinberg Igor , Elias Yuval , Margel Shlomo
Published: December 2020

Proteinoids are non-toxic biodegradable polymers prepared by thermal step-growth polymerization of amino acids. Here, P(RGD) proteinoids and proteinoid nanocapsules (NCs) based on D-arginine, glycine, and L-aspartic acid were synthesized and characterized for targeted tumor therapy. Doxorubicin (Dox), a chemotherapeutic drug used for treatment of a wide range of cancers, known for its adverse side effects, was encapsulated during self-assembly to form Dox/P(RGD) NCs. In addition, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), which can initiate apoptosis in most tumor cells but undergoes fast enzyme degradation, was stabilized by covalent conjugation to hollow P(RGD) NCs. The effect of polyethylene glycol (PEG) conjugation was also studied. Cytotoxicity tests on CAOV-3 ovarian cancer cells demonstrated that Dox/P(RGD) and TRAIL-P(RGD) NCs were as effective as free Dox and TRAIL with cell viability of 2% and 10%, respectively, while PEGylated NCs were less effective. Drug-bearing P(RGD) NCs offer controlled release with reduced side effects for improved therapy.
Characterization of Porcine Urinary Bladder Matrix Hydrogels from Sodium Dodecyl Sulfate Decellularization Method
Authors: Kao Chen-Yu , Nguyen Huynh-Quang-Dieu , Weng Yu-Chuan
Published: December 2020

Urinary bladder matrix (UBM) is one of the most studied extracellular matrixes (ECM) in the tissue engineering field. Although almost all of the UBM hydrogels were prepared by using peracetic acid (PAA), recent studies indicated that PAA was not a trustworthy way to decellularize UBM. A stronger detergent, such as sodium dodecyl sulfate (SDS), may help tackle this issue; however, its effects on the hydrogels’ characteristics remain unknown. Therefore, the objective of this study was to develop a more reliable protocol to decellularize UBM, using SDS, and to compare the characteristics of hydrogels obtained from this method to the widely employed technique, using PAA. The results indicated that SDS was superior to PAA in decellularization efficacy. Different decellularization methods led to dissimilar gelation kinetics; however, the methods did not affect other hydrogel characteristics in terms of biochemical composition, surface morphology and rheological properties. The SDS-treated hydrogels possessed excellent cytocompatibility in vitro. These results showed that the SDS decellularization method could offer a more stable and safer way to obtain acellular UBM, due to reducing immunogenicity. The hydrogels prepared from this technique had comparable characteristics as those from PAA and could be a potential candidate as a scaffold for tissue remodeling.
On the Heuristic Procedure to Determine Processing Parameters in Additive Manufacturing Based on Materials Extrusion
Authors: Bakradze Georgijs , Arājs Egīls , Gaidukovs Sergejs , Thakur Vijay Kumar
Published: December 2020

We present a heuristic procedure for determining key processing parameters (PPs) in materials-extrusion-based additive manufacturing processes. The concept relies on a design-of-experiment approach and consists of eleven “test objects” to determine the optimal combinations of key PPs values, starting with the PPs for printing the first layer and progressing to more complex geometric features, e.g., “bridges”. In each of the test objects, several combinations of the known PPs’ values are used, and only the values resulting in the best printed-part quality are selected for the following tests. The concept is intrinsically insensitive to different artefacts of the additive manufacturing machine (e.g., discrepancies between the nominal and actual nozzle diameters, and improper calibration of the feeding screws) and the optimal values of key PPs for manufacturing defect-free parts under the actual processing conditions can be determined. We validated the proposed procedure for two common commercial polymer feedstock materials, and we show that, by using the proposed procedure, it is possible to reduce the optimization time down to several hours, as well as to reduce the amount of consumed feedstock material. Tensile tests revealed a strong effect of amorphous and semi-crystalline nature of the polymer on the results of optimization. To the best of our knowledge, this is the first attempt to describe a systematic approach for optimizing PPs for materials extrusion-based additive manufacturing processes without relying on statistical data analysis or virtual simulations. The concept was implemented as a web-tool 3DOptimizer®.
Enhanced Hydroxide Conductivity and Dimensional Stability with Blended Membranes Containing Hyperbranched PAES/Linear PPO as Anion Exchange Membranes
Authors: Kim Sang Hee , Lee Kyu Ha , Chu Ji Young , Kim Ae Rhan , Yoo Dong Jin
Published: December 2020

A series of novel blended anion exchange membranes (AEMs) were prepared with hyperbranched brominated poly(arylene ether sulfone) (Br-HB-PAES) and linear chloromethylated poly(phenylene oxide) (CM-PPO). The as-prepared blended membranes were fabricated with different weight ratios of Br-HB-PAES to CM-PPO, and the quaternization reaction for introducing the ionic functional group was performed by triethylamine. The Q-PAES/PPO-XY (quaternized-PAES/PPO-XY) blended membranes promoted the ion channel formation as the strong hydrogen bonds interconnecting the two polymers were maintained, and showed an improved hydroxide conductivity with excellent thermal behavior. In particular, the Q-PAES/PPO-55 membrane showed a very high hydroxide ion conductivity (90.9 mS cm−1) compared to the pristine Q-HB-PAES membrane (32.8 mS cm−1), a result supported by the morphology of the membrane as determined by the AFM analysis. In addition, the rigid hyperbranched structure showed a suppressed swelling ratio of 17.9–24.9% despite an excessive water uptake of 33.2–50.3% at 90 °C, and demonstrated a remarkable alkaline stability under 2.0 M KOH conditions over 1000 h.
Comparison of Conformational Phase Behavior for Flexible and Semiflexible Polymers
Authors: Aierken Dilimulati , Bachmann Michael
Published: December 2020

We employ the recently introduced generalized microcanonical inflection point method for the statistical analysis of phase transitions in flexible and semiflexible polymers and study the impact of the bending stiffness upon the character and order of transitions between random-coil, globules, and pseudocrystalline conformations. The high-accuracy estimates of the microcanonical entropy and its derivatives required for this study were obtained by extensive replica-exchange Monte Carlo simulations. We observe that the transition behavior into the compact phases changes qualitatively with increasing bending stiffness. Whereas the Θ collapse transition is less affected, the first-order liquid-solid transition characteristic for flexible polymers ceases to exist once bending effects dominate over attractive monomer-monomer interactions.
Scaling Theory of a Polymer Ejecting from a Cavity into a Semi-Space
Authors: Hsiao Pai-Yi
Published: December 2020

A two-stage model is developed in order to understand the scaling behaviors of single polymers ejecting from a spherical cavity through a nanopore. The dynamics of ejection is derived by balancing the free energy change with the energy dissipation during a process. The ejection velocity is found to vary with the number of monomers in the cavity, m, as mz1/(Nx1D3z1) at the confined stage, and it turns to be m−z2 at the non-confined stage, where N is the chain length and D the cavity diameter. The exponents are shown to be z1=(3ν−1)−1, z2=2ν and x1=1/3, with ν being the Flory exponent. The profile of the velocity is carefully verified by performing Langevin dynamics simulations. The simulations further reveal that, at the starting point, the decreasing of m can be stalled for a good moment. It suggests the existence of a pre-stage that can be explained by using the concept of a classical nucleation theory. By trimming the pre-stage, the ejection time are properly studied by varying N, D, and ϕ0 (the initial volume fraction). The scaling properties of the nucleation time are also analyzed. The results fully support the predictions of the theory. The physical pictures are given for various ejection conditions that cover the entire parameter space.
Polymer Based Bioadhesive Biomaterials for Medical Application—A Perspective of Redefining Healthcare System Management
Authors: Saha Nibedita , Saha Nabanita , Sáha Tomas , Toksoy Öner Ebru , Brodnjak Urška Vrabič , Redl Heinz , von Byern Janek , Sáha Petr
Published: December 2020

This article deliberates about the importance of polymer-based bioadhesive biomaterials’ medical application in healthcare and in redefining healthcare management. Nowadays, the application of bioadhesion in the health sector is one of the great interests for various researchers, due to recent advances in their formulation development. Actually, this area of study is considered as an active multidisciplinary research approach, where engineers, scientists (including chemists, physicists, biologists, and medical experts), material producers and manufacturers combine their knowledge in order to provide better healthcare. Moreover, while discussing the implications of value-based healthcare, it is necessary to mention that health comprises three main domains, namely, physical, mental, and social health, which not only prioritize the quality healthcare, but also enable us to measure the outcomes of medical interventions. In addition, this conceptual article provides an understanding of the consequences of the natural or synthetic polymer-based bioadhesion of biomaterials, and its significance for redefining healthcare management as a novel approach. Furthermore, the research assumptions highlight that the quality healthcare concept has recently become a burning topic, wherein healthcare service providers, private research institutes, government authorities, public service boards, associations and academics have taken the initiative to restructure the healthcare system to create value for patients and increase their satisfaction, and lead ultimately to a healthier society.
Municipal Solid Waste as Secondary Resource: Selectively Separating Cu(II) from Highly Saline Fly Ash Extracts by Polymer-Assisted Ultrafiltration
Authors: Hettenkofer Christine , Fromm Stephan , Schuster Michael
Published: December 2020

Urban mining from fly ash resulting from municipal solid waste incineration (MSWI) is becoming more and more important due to the increasing scarcity of supply-critical metals. Metal extraction from acid fly ash leaching has already been established. In this context selective Cu recovery is still a challenge. Therefore, our purpose was the separation of Cu(II) from MSWI fly ash extracts by polymer-assisted ultrafiltration (PAUF). We investigated three polyethyleneimines (PEIs) with regard to metal retention, Cu(II) selectivity, Cu(II) loading capacity, and the viscosity of the PEI containing solutions. A demanding challenge was the highly complex matrix of the fly ash extracts, which contain up to 16 interfering metal ions in high concentrations and a chloride content of 60 g L−1. Overcoming that, Cu(II) was selectively enriched and separated from real fly ash extract at pH 3.0. At pH 1.0, a PEI-free Cu(II) concentrate was obtained and PEIs could be regenerated for reuse in further separation cycles. The PAUF conditions developed at laboratory scale were successfully transferred to pilot scale, and hyperbranched PEI (HB-PEI) was found to be the most suitable reagent for PAUF in a technical scale. Moreover, HB-PEI enables photometric control of the Cu(II) enrichment.
Seng Zhao’s The Immutability of Things and Responses to It in the Late Ming Dynasty
Authors: Liu Yu , Anderl Christoph , Dessein Bart
Published: December 2020

Seng Zhao and his collection of treatises, the Zhao lun, have enjoyed a particularly high reputation in the history of Chinese Buddhism. One of these treatises, The Immutability of Things, employs the Madhyamaka argumentative method of negating dualistic concepts to demonstrate that, while “immutability” and “mutability” coexist as the states of phenomenal things, neither possesses independent self-nature. More than a thousand years after this text was written, Zhencheng’s intense criticism of it provoked fierce reactions among a host of renowned scholar–monks. This paper explores Zhencheng’s main points as well as the perspectives and motives of his principal adversaries in order to shed light on the nature of philosophical discourse during the late Ming dynasty.
Effects of Patchiness on Surface Soil Moisture of Alpine Meadow on the Northeastern Qinghai-Tibetan Plateau: Implications for Grassland Restoration
Authors: Zhang Wei , Yi Shuhua , Qin Yu , Sun Yi , Shangguan Donghui , Meng Baoping , Li Meng , Zhang Jianguo
Published: December 2020

Surface soil moisture (SSM) is a key limiting factor for vegetation growth in alpine meadow on the Qinghai-Tibetan Plateau (QTP). Patches with various sizes and types may cause the redistribution of SSM by changing soil hydrological processes, and then trigger or accelerate alpine grassland degradation. Therefore, it is vital to understand the effects of patchiness on SSM at multi-scales to provide a reference for alpine grassland restoration. However, there is a lack of direct observational evidence concerning the role of the size and type of patches on SSM, and little is known about the effects of patches pattern on SSM at plot scale. Here, we first measured SSM of typical patches with different sizes and types at patch scale and investigated their patterns and SSM spatial distribution through unmanned aerial vehicle (UAV)-mounted multi-type cameras at plot scale. We then analyzed the role of the size and type of patchiness on SSM at both patch and plot scales. Results showed that: (1) in situ measured SSM of typical patches was significantly different (P < 0.01), original vegetation patch (OV) had the highest SSM, followed by isolate vegetation patch (IV), small bare patch (SP), medium bare patch (MP) and large bare patch (LP); (2) the proposed method based on UAV images was able to estimate SSM (0–40 cm) with a satisfactory accuracy (R2 = 0.89, P < 0.001); (3) all landscape indices of OV, with the exception of patch density, were positively correlated with SSM at plot scale, while most of the landscape indices of LP and IV showed negative correlations (P < 0.05). Our results indicated that patchiness intensified the spatial heterogeneity of SSM and potentially accelerated the alpine meadow degradation. Preventing the development of OV into IV and the expansion of LP is a critical task for alpine meadow management and restoration.
ECOSTRESS and CIMIS: A Comparison of Potential and Reference Evapotranspiration in Riverside County, California
Authors: Kohli Gurjot , Lee Christine M. , Fisher Joshua B. , Halverson Gregory , Variano Evan , Jin Yufang , Carney Daniel , Wilder Brenton A. , Kinoshita Alicia M.
Published: December 2020

The ECOsystem Spaceborne Thermal Radiometer Experiment on Space Station (ECOSTRESS) provides remotely-sensed estimates of evapotranspiration at 70 m spatial resolution every 1–5 days, sampling across the diurnal cycle. This study, in partnership with an operational water management organization, the Eastern Municipal Water District (EMWD) in Southern California, was conducted to evaluate estimates of evapotranspiration under ideal conditions where water is not limited. EMWD regularly uses a ground-based network of reference evapotranspiration (ETo) from the California Irrigation Management Information System (CIMIS); yet, there are gaps in spatial coverage and questions of spatial representativeness and consistency. Space-based potential evapotranspiration (PET) estimates, such as those from ECOSTRESS, provide consistent spatial coverage. We compared ECOSTRESS ETo (estimated from PET) to CIMIS ETo at five CIMIS sites in Riverside County, California from July 2018–June 2020. We found strong correlations between CIMIS ETo and ECOSTRESS ETo across all five sites (R2 = 0.89, root mean square error (RMSE) = 0.11 mm hr−1). Both CIMIS and ECOSTRESS ETo captured similar seasonal patterns through the study period as well as diurnal variability. There were site-specific differences in the relationship between ECOSTRESS AND CIMIS, in part due to spatial heterogeneity around the station site. Consequently, careful examination of landscapes surrounding CIMIS sites must be considered in future comparisons. These results indicate that ECOSTRESS successfully retrieves PET that is comparable to ground-based reference ET, highlighting the potential for providing observation-driven guidance for irrigation management across spatial scales.