bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimal Breeding Strategy for Livestock with a Dynamic Price
Authors: Wang Leishi , Li Mingtao , Pei Xin , Zhang Juan
Published: May 2022

China’s livestock output has been growing, but domestic livestock products such as beef, mutton and pork have been unable to meet domestic consumers’ demands. The imbalance between supply and demand causes unstable livestock prices and affects profits on livestock. Therefore, the purpose of this paper is to provide the optimal breeding strategy for livestock farmers to maximize profits and adjust the balance between supply and demand. Firstly, when the price changes, livestock farmers will respond in two ways: by not adjusting the scale of livestock with the price or adjusting the scale with the price. Therefore, combining the model of price and the behavior of livestock farmers, two livestock breeding models were established. Secondly, we proposed four optimal breeding strategies based on the previously studied models and the main research method is Pontryagin’s Maximum Principle. Optimal breeding strategies are achieved by controlling the growth and output of livestock. Further, their existence was verified. Finally, we simulated two situations and found the most suitable strategy for both situations by comparing profits of four strategies. From that, we obtained several conclusions: The optimal strategy under constant prices is not always reasonable. The effect of price on livestock can promote a faster balance. To get more profits, the livestock farmers should adjust the farm’s productivity reasonably. It is necessary to calculate the optimal strategy results under different behaviors.
Sequence-to-Sequence Remaining Useful Life Prediction of the Highly Maneuverable Unmanned Aerial Vehicle: A Multilevel Fusion Transformer Network Solution
Authors: Ai Shaojie , Song Jia , Cai Guobiao
Published: May 2022

The remaining useful life (RUL) of the unmanned aerial vehicle (UAV) is primarily determined by the discharge state of the lithium-polymer battery and the expected flight maneuver. It needs to be accurately predicted to measure the UAV’s capacity to perform future missions. However, the existing works usually provide a one-step prediction based on a single feature, which cannot meet the reliability requirements. This paper provides a multilevel fusion transformer-network-based sequence-to-sequence model to predict the RUL of the highly maneuverable UAV. The end-to-end method is improved by introducing the external factor attention and multi-scale feature mining mechanism. Simulation experiments are conducted based on a high-fidelity quad-rotor UAV electric propulsion model. The proposed method can rapidly predict more precisely than the state-of-the-art. It can predict the future RUL sequence by four-times the observation length (32 s) with a precision of 83% within 60 ms.
The Role of Seawater and Saline Solutions in Treatment of Upper Respiratory Conditions
Authors: Štanfel Danijela , Kalogjera Livije , Ryazantsev Sergej V. , Hlača Kristina , Radtsig Elena Y. , Teimuraz Rashidov , Hrabač Pero
Published: May 2022

The history of saline nasal irrigation (SNI) is indeed a long one, beginning from the ancient Ayurvedic practices and gaining a foothold in the west at the beginning of the 20th century. Today, there is a growing number of papers covering the effects of SNI, from in vitro studies to randomized clinical trials and literature overviews. Based on the recommendations of most of the European and American professional associations, seawater, alone or in combination with other preparations, has its place in the treatment of numerous conditions of the upper respiratory tract (URT), primarily chronic (rhino)sinusitis, allergic rhinitis, acute URT infections and postoperative recovery. Additionally, taking into account its multiple mechanisms of action and mounting evidence from recent studies, locally applied seawater preparations may have an important role in the prevention of viral and bacterial infections of the URT. In this review we discuss results published in the past years focusing on seawater preparations and their use in clinical and everyday conditions, since such products provide the benefits of additional ions vs. saline, have an excellent safety profile and are recommended by most professional associations in the field of otorhinolaryngology.
Cyclodepsipeptides: Isolation from Endophytic Fungi of Sarcophyton ehrenbergi and Verification of Their Larvicidal Activity via In-Vitro and In-Silico Studies
Authors: Singab Abdel Nasser B. , Mostafa Nada M. , Elkhawas Yasmin A. , Al-Sayed Eman , Bishr Mokhtar M. , Elissawy Ahmed M. , Elnaggar Mohamed S. , Fawzy Iten M. , Salama Osama M. , Tsai Yi-Hong , Chang Fang-Rong
Published: May 2022

Culex pipiens mosquitoes are vectors to many viruses and can transmit diseases such as filariasis and avian malaria. The present study evaluated the larvicidal activity of marine-derived endophytic fungi Aspergillus nomius and Aspergillus flavus from the soft coral Sarcophyton ehrenbergi along with two known cyclodepsipeptide compounds, scopularide A (1) and B (2), isolated from A. flavus extract, against third-instar larvae of C. pipiens, using distilled water as a negative control and toosenedanin as a positive control. The structures of the isolated compounds were confirmed by various spectroscopic analyses. The lethal concentrations (LC50 and LC90) were calculated by probit analysis. Scopularide A was the most potent after 96 h treatment, with LC50 and LC90 values of 58.96 and 994.31 ppm, respectively, and with 82.66% mortality at a concentration of 300 ppm. To unravel the biochemical mechanism of the tested extracts and compounds, their effects against protease, chitinase, phenoloxidases and lipase enzymes from the whole-body tissue of C. pipiens were evaluated after 72 h treatment at LC50 dose. Superior activity was observed for A. flavus extract against all tested enzymes. A molecular docking study was conducted for scopularide A and B on the four tested enzymes, to further verify the observed activity. Results revealed good binding affinities for both compounds as compared to the docked ligands, mainly via a number of hydrogen bonds. This was the first study to report the isolation of endophytic fungi A. flavus and A. nomius from the marine soft coral S. ehrenbergi. The endophytic fungal extract of A. flavus was found to be a promising source for a natural larvicidal agent against C. pipiens populations.
Deep Fat Saving Elevation of the Superficial Circumflex Iliac Artery Perforator Flap
Authors: Fuse Yuma , Yoshimatsu Hidehiko , Karakawa Ryo , Yano Tomoyuki
Published: May 2022

Background and Objectives: Prolonged drain stay and lymphorrhea are often problems at the donor site of the superficial circumflex iliac artery perforator (SCIP) flap. This study aimed to introduce a novel technique of the SCIP flap elevation: Deep Fat Saving (DFS) technique. Materials and Methods: Thirty-two patients who underwent the SCIP flap transfer were divided based on the flap-elevated layer: above the deep fascia or the Camper fascia saving the deep fat. The duration of drain stay and the rates of flap survival and donor-site complications were compared between the groups. The inverse probability weighting (IPW) method was conducted to balance confounders. Results: By IPW, two balanced pseudo-populations were created: DFS = 33.9 and Conventional = 31.3. There were no significant differences in the rate of flap survival (DFS: 100% verses Conventional: 95.8%, p = 0.32) and donor site complications (DFS: 2.4% versus Conventional: 1.3%, p = 0.68, respectively). The duration of drain stay was shorter in the DFS group (weighted median: 6 versus 8 days; weighted difference: −1.6 days (95% confidence interval: −2.8 to −0.4), p = 0.01). Conclusions: An SCIP flap can be reliably harvested using the Deep Fat Saving technique.
Duplex Analysis of Cannulated Vessels in Peripheral Veno-Arterial Extracorporeal Membrane Oxygenation
Authors: Simons Jorik , Agricola Sandra , Smets Jeroen , Metz Renske , Mariani Silvia , Vleugels Marie-José , Smeets Reinier R. , van Mook Walther N. K. A. , Mees Barend , Lorusso Roberto
Published: May 2022

Background and objectives: Veno-arterial extracorporeal membrane oxygenation (V-A ECMO) cannulas have major repercussions on vascular hemodynamics that can potentially lead to limb ischemia. Duplex ultrasound enables the non-invasive analysis of vascular hemodynamics. This study aims to describe the duplex parameters of the femoral vessels during V-A ECMO support, investigate differences between cannulated and non-cannulated vessels, and analyze the variations in the case of limb ischemia and intra-aortic balloon pumps (IABPs). Methods: Nineteen adults (≥18 years), supported with femoro-femoral V-A ECMO, underwent a duplex analysis of the superficial femoral arteries (SFAs) and veins (FVs). Measured parameters included flow velocities, waveforms, and vessel diameters. Results: 89% of patients had a distal perfusion cannula during duplex analysis and 21% of patients developed limb ischemia. The mean peak systolic flow velocity (PSV) and end-diastolic flow velocity (EDV) of the SFAs on the cannulated side were, respectively, 42.4 and 21.4 cm/s. The SFAs on the non-cannulated side showed a mean PSV and EDV of 87.4 and 19.6 cm/s. All SFAs on the cannulated side had monophasic waveforms, whereas 63% of the SFAs on the non-cannulated side had a multiphasic waveform. Continuous/decreased waveforms were seen in 79% of the FVs on the cannulated side and 61% of the waveforms of the contralateral veins were respirophasic. The mean diameter of the FVs on the cannulated side, in patients who developed limb ischemia, was larger compared to the FVs on the non-cannulated side with a ratio of 1.41 ± 0.12. The group without limb ischemia had a smaller ratio of 1.03 ± 0.25. Conclusions: Femoral cannulas influence flow velocities in the cannulated vessels during V-A ECMO and major waveforms alternations can be seen in all SFAs on the cannulated side and most FVs on the cannulated side. Our data suggest possible venous stasis in the FV on the cannulated side, especially in patients suffering from limb ischemia.
Tomographic Imaging of Mucociliary Clearance Following Maxillary Sinus Augmentation: A Case Series
Authors: Park Won-Bae , Cho Nam-Jun , Kang Philip
Published: May 2022

Mucociliary clearance (MCC) allows ventilation of graft particles that are displaced through a perforated Schneiderian membrane during maxillary sinus augmentation (MSA). However, it is very rarely confirmed by cone-beam computed tomographic (CBCT) images. It is not yet known how long the dislodged bone graft particles remain in the maxillary sinus or how quickly they are ventilated after MSA. The purpose of these case reports is to introduce tomographic imaging of ventilation of bone graft particles displaced through a perforated Schneiderian membrane after MSA. Four patients, who needed implant placement in the posterior maxilla, received MSA, during which the Schneiderian membrane was perforated but was not repaired. Therefore, some bone graft particles were dislocated into the sinus cavity. The sizes of the perforated membranes were measured and recorded. CBCT scans were taken at multiple time points after the surgery to visualize and trace the ejected material. In addition, the time from when the bone graft substitute was delivered to the sinus until the CBCT scans were taken was recorded. The expelled bone graft particles migrated to the ostium along the sinus wall immediately after MSA on CBCT images taken immediately after the surgery. No displaced graft particles were observed in the maxillary sinus on CBCT scans after 1 week. The CBCT scans at 6 months showed no unusual radiographic images. Within the limitations of the case reports, tomographic imaging revealed an MCC system that allows displaced graft particles to be ventilated into the ostium very early during MSA healing and not stagnate in the maxillary sinus.
Proteobacteria and Firmicutes Secreted Factors Exert Distinct Effects on Pseudomonas aeruginosa Infection under Normoxia or Mild Hypoxia
Authors: Charalambous Anna , Grivogiannis Evangelos , Dieronitou Irene , Michael Christina , Rahme Laurence , Apidianakis Yiorgos
Published: May 2022

Microbiota may alter a pathogen’s virulence potential at polymicrobial infection sites. Here, we developed a multi-modal Drosophila assay, amenable to the assessment of human bacterial interactions using fly survival or midgut regeneration as a readout, under normoxia or mild hypoxia. Deploying a matrix of 12 by 33 one-to-one Drosophila co-infections via feeding, we classified bacterial interactions as neutral, synergistic, or antagonistic, based on fly survival. Twenty six percent of these interactions were antagonistic, mainly occurring between Proteobacteria. Specifically, Pseudomonas aeruginosa infection was antagonized by various Klebsiella strains, Acinetobacter baumannii, and Escherichia coli. We validated these interactions in a second screen of 7 by 34 one-to-one Drosophila co-infections based on assessments of midgut regeneration, and in bacterial co-culture test tube assays, where antagonistic interactions depended on secreted factors produced upon high sugar availability. Moreover, Enterococci interacted synergistically with P. aeruginosa in flies and in test tubes, enhancing the virulence and pyocyanin production by P. aeruginosa. However, neither lactic acid bacteria nor their severely hypoxic culture supernatants provided a survival benefit upon P. aeruginosa infection of flies or mice, respectively. We propose that at normoxic or mildly hypoxic sites, Firmicutes may exacerbate, whereas Proteobacteria secreted factors may ameliorate, P. aeruginosa infections.
Automated Cancer Diagnostics via Analysis of Optical and Chemical Images by Deep and Shallow Learning
Authors: Isberg Olof Gerdur , Giunchiglia Valentina , McKenzie James S. , Takats Zoltan , Jonasson Jon Gunnlaugur , Bodvarsdottir Sigridur Klara , Thorsteinsdottir Margret , Xiang Yuchen
Published: May 2022

Optical microscopy has long been the gold standard to analyse tissue samples for the diagnostics of various diseases, such as cancer. The current diagnostic workflow is time-consuming and labour-intensive, and manual annotation by a qualified pathologist is needed. With the ever-increasing number of tissue blocks and the complexity of molecular diagnostics, new approaches have been developed as complimentary or alternative solutions for the current workflow, such as digital pathology and mass spectrometry imaging (MSI). This study compares the performance of a digital pathology workflow using deep learning for tissue recognition and an MSI approach utilising shallow learning to annotate formalin-fixed and paraffin-embedded (FFPE) breast cancer tissue microarrays (TMAs). Results show that both deep learning algorithms based on conventional optical images and MSI-based shallow learning can provide automated diagnostics with F1-scores higher than 90%, with the latter intrinsically built on biochemical information that can be used for further analysis.
Monitoring the Starvation–Survival Response of Edwardsiella piscicida and E. tarda in Freshwater Microcosms, at Various Temperatures
Authors: Esteve Consuelo , Alcaide Elena
Published: May 2022

Edwardsiella piscicida is an important fish pathogen responsible for economic losses in global aquaculture, and E. tarda is also a human zoonotic pathogen. In this study, the survival of E. piscicida and E. tarda strains kept in filtered and sterilized lake water microcosms was investigated during a 20-week period at 7 °C, 15 °C and 25 °C, as well as its pathogenicity retention during a starvation period. E. tarda V43.2 stayed culturable for 6 weeks at 7 °C, 9 weeks at 25 °C and 12 weeks at 15 °C. Both E. piscicida strains (V12.1 and V57.2) stayed culturable even longer, for at least 12 weeks at 7 °C, 15 °C and 25 °C under the same starvation conditions. After Edwardsiella cells entered into the VBNC state, some became shorter and ”rounded up,” but others aggregated and retained a short rod shape. Aggregates of Edwardsiella cells were common throughout the VBNC period, and a well-formed biofilm was observed for all tested strains at the end of the experiment. The growth capacity of VBNC cells was restored by cultivating microcosm water samples in LB broth at 28 °C. Resuscitated E. piscicida cells were as virulent for the European eel as the controls. Natural waters can be a reservoir for Edwardsiella, and its underestimation in environmental samples poses a risk to public health and aquaculture.
Gut Microbiota Associated with Clinical Relapse in Patients with Quiescent Ulcerative Colitis
Authors: Kitae Hiroaki , Takagi Tomohisa , Naito Yuji , Inoue Ryo , Azuma Yuka , Torii Takashi , Mizushima Katsura , Doi Toshifumi , Inoue Ken , Dohi Osamu , Yoshida Naohisa , Kamada Kazuhiro , Uchiyama Kazuhiko , Ishikawa Takeshi , Konishi Hideyuki , Itoh Yoshito
Published: May 2022

The microbiota associated with relapse in patients with quiescent ulcerative colitis (qUC) remains unclear. Our objective was to analyze the fecal microbiota of Japanese patients with qUC and identify the relapse-associated microbiota. In this study, 59 patients with qUC and 59 healthy controls (HCs) were enrolled (UMIN 000019486), and their fecal microbiota was compared using 16S rRNA gene amplicon sequencing. We followed their clinical course up to 3.5 years and analyzed the relapse-associated microbiota. Potential functional changes in the fecal microbiota were evaluated using PICRUSt software and the Kyoto Encyclopedia of Genes and Genomes database. There were significant differences in fecal microbiota diversity between HC and qUC subjects, with 13 taxa characterizing each subject. Despite no significant difference in variation of microbiota in a single sample (α diversity) between patients in sustained remission and relapsed patients, the variation in microbial communities between samples (β diversity) was significantly different. Prevotella was more abundant in the sustained remission patients, whereas Faecalibacterium and Bifidobacterium were more abundant in the relapsed patients. We clustered the entire cohort into four clusters, and Kaplan–Meier analysis revealed the subsequent clinical course of each cluster was different. We identified 48 metabolic pathways associated with each cluster using linear discriminant analysis effect size. We confirmed the difference in microbiota between patients with qUC and HCs and identified three genera associated with relapse. We found that the clusters based on these genera had different subsequent clinical courses and activated different metabolic pathways.
Trust Your Gut: The Association of Gut Microbiota and Liver Disease
Authors: Manzoor Ridda , Ahmed Weshah , Afify Nariman , Memon Mashal , Yasin Maryam , Memon Hamda , Rustom Mohammad , Al Akeel Mohannad , Alhajri Noora
Published: May 2022

The gut microbiota composition is important for nutrient metabolism, mucosal barrier function, immunomodulation, and defense against pathogens. Alterations in the gut microbiome can disturb the gut ecosystem. These changes may lead to the loss of beneficial bacteria or an increase in potentially pathogenic bacteria. Furthermore, these have been shown to contribute to the pathophysiology of gastrointestinal and extra-intestinal diseases. Pathologies of the liver, such as non-alcoholic liver disease, alcoholic liver disease, cirrhosis, hepatocellular carcinoma, autoimmune hepatitis, viral hepatitis, and primary sclerosing cholangitis have all been linked to changes in the gut microbiome composition. There is substantial evidence that links gut dysbiosis to the progression and complications of these pathologies. This review article aimed to describe the changes seen in the gut microbiome in liver diseases and the association between gut dysbiosis and liver disease, and finally, explore treatment options that may improve gut dysbiosis in patients with liver disease.
Sodium Citrate Alleviates Virulence in Pseudomonas aeruginosa
Authors: Khayat Maan T. , Ibrahim Tarek S. , Khayyat Ahdab N. , Alharbi Majed , Shaldam Moataz A. , Mohammad Khadijah A. , Khafagy El-Sayed , El-damasy Dalia A. , Hegazy Wael A. H. , Abbas Hisham A.
Published: May 2022

The development of bacterial resistance is an insistent global health care issue, especially in light of the dwindled supply of new antimicrobial agents. This mandates the development of new innovative approaches to overcome the resistance development obstacle. Mitigation of bacterial virulence is an interesting approach that offers multiple advantages. Employing safe chemicals or drugs to mitigate bacterial virulence is an additive advantage. In the current study, the in vitro antivirulence activities of citrate were evaluated. Significantly, sodium citrate inhibited bacterial biofilm formation at sub-MIC concentrations. Furthermore, sodium citrate decreased the production of virulence factors protease and pyocyanin and diminished bacterial motility. Quorum sensing (QS) is the communicative system that bacterial cells utilize to communicate with each other and regulate the virulence of the host cells. In the present study, citrate in silico blocked the Pseudomonas QS receptors and downregulated the expression of QS-encoding genes. In conclusion, sodium citrate showed a significant ability to diminish bacterial virulence in vitro and interfered with QS; it could serve as a safe adjuvant to traditional antibiotic treatment for aggressive resistant bacterial infections such as Pseudomonas aeruginosa infections.
Microplastics’ Occurrence in Edible Fish Species (Mullus barbatus and M. surmuletus) from an Italian Marine Protected Area
Authors: Felline Serena , Piccardo Manuela , De Benedetto Giuseppe Egidio , Malitesta Cosimino , Terlizzi Antonio
Published: May 2022

This study reports on the presence of microplastics in the gastrointestinal tracts and livers of demersal fish (the mullet, Mullus spp.) from a Marine Protected Area (Porto Cesareo) along the Ionian Sea coast (Apulia, Southern Italy). The results showed microplastic ingestion in more than 60% of specimens analyzed with an average of three items per fish and average levels in red mullets being almost twice as high as the average in the congeneric striped red mullets. The dominant polymers identified by Attenuated Total ReflectanceFourier Transform Infrared spectroscopy (ATR-FTIR) and Prolysis Gas Chromatography/Mass Spectrometry (Py-GCMS) analysis were polyethylene and polystyrene. Results can be used to set baseline levels for the assessment of microplastic pollution useful for the implementation of the Marine Strategy Framework Directive (MSFD) descriptor 10 in the Italian coast of Ionian Sea.
Enhancement of Dissolving Capacity and Reducing Gastric Mucosa Irritation by Complex Formation of Resibufogenin with β-Cyclodextrin or 2-Hydroxypropyl-β-cyclodextrin
Authors: Liu Nan , Chen Huan-Ping , Yang Zi-Meng , Xia Ming-Yu , Wang Dong , Zang Ling-He , Liu Dong-Chun
Published: May 2022

Resibufogenin (RBG) is a natural medicinal ingredient with promising cardiac protection and antitumor activity. However, poor solubility and severe gastric mucosa irritation restrict its application in the pharmaceutical field. In this study, the inclusion complex of RBG with β-cyclodextrin (β-CD) and 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) was prepared using the co-evaporation method, and the molar ratio of RBG to CD was determined to be approximately 1:2 by continuous variation plot for both CDs. The formation of inclusion complexes between RBG and each CD (RBG/β-CD and RBG/HP-β-CD) was evaluated by phase solubility study, Fourier transform infrared spectroscopy, and thin-layer chromatography. Powder X-ray diffraction and differential scanning calorimetry confirmed drug amorphization and encapsulation in the molecular cage for both CDs. Moreover, the inclusion complexes’ morphologies were observed using scanning electron microscopy. The dissolution rate of the inclusion complexes was markedly improved compared to that of RBG, and the complexes retained their antitumor activity, as shown in the in vitro cytotoxicity assay on a human lung adenocarcinoma cancer (A549) cell line. Moreover, less gastric mucosal irritation was observed for the inclusion complex. Thus, the inclusion complex should be considered a promising strategy for the delivery of poorly water-soluble anticancer agents, such as RBG.
One-Chip Isolation of Drug-Resistant Acute Myeloid Leukemia Cells with CXCR4-Targeted Magnetic Fluorescent Nanoprobes
Authors: Wang Fan , Jiang Yuqi , Wang Luhai , Chen Yi , Zhang Yu , Ma Ming
Published: May 2022

Drug resistance and relapse lead to high mortality in acute myeloid leukemia, and studies have shown that CXCR4 overexpression is highly correlated with poor prognosis and drug resistance in leukemia cells. Isolation and detection of AML cells with CXCR4 overexpression will be crucial to the treatment of AML. In this paper, magnetic nanoparticles were firstly prepared successfully by high-temperature thermal decomposition method, and then characterized by TEM, VSM and DLS. Subsequently CXCR4-targeted magnetic fluorescent nanoprobes conjugated with antibody 12G5 were constructed by stepwise coupling. In cell experiments, the obtained probes demonstrated excellent targeting efficacy to CXCR4 overexpressed AML cells HL-60. In addition, HL-60 cells labelled with the magnetic probes can be magnetic isolated successfully in one microfluidics chip, with efficiency of 82.92 ± 7.03%. Overall, this method utilizes the superiority of superparamagnetic nanomaterials and microfluidic technology to achieve the enrichment and capture of drug-resistant cells in a microfluidic chip, providing a new idea for the isolation and detective of drug-resistant acute myeloid leukemia cells.
Pinning Potential of the Self-Assembled Artificial Pinning Centers in Nanostructured YBa2Cu3O7−x Superconducting Films
Authors: Ivan Ion , Ionescu Alina M. , Crisan Daniel N. , Andrei Andreea , Galluzzi Armando , Polichetti Massimiliano , Mosqueira Jesus , Crisan Adrian
Published: May 2022

For high-field power applications of high-temperature superconductors, it became obvious in recent years that nano-engineered artificial pinning centers are needed for increasing the critical current and pinning potential. As opposed to the artificial pinning centers obtained by irradiation with various particles, which is a quite expensive approach, we have studied superconducting samples having self-assembled defects, created during the sample fabrication, that act as effective pinning centers. We introduced a simple, straight-forward method of estimating the frequency-dependent critical current density by using frequency-dependent AC susceptibility measurements, in fixed temperatures and DC magnetic fields, from the positions of the maxima in the dependence of the out-of-phase susceptibility on the amplitude of AC excitation magnetic field. The results are compatible with a model that stipulates a logarithmic dependence of the pinning potential on the probing current. A mathematical derivation allowed us to estimate from the experimental data the pinning potentials in various samples, and in various DC magnetic fields. The resulted values indicate large pinning potentials, leading to very small probability of magnetic flux escaping the pinning wells, hence, leading to very high critical currents in high magnetic fields.
Achieving Up-Conversion Amplified Spontaneous Emission through Spin Alignment between Coherent Light-Emitting Excitons in Perovskite Microstructures
Authors: Zhu Xixiang , Xie Yulin , Ma Xiaoling
Published: May 2022

Metal hybrid perovskites have presented interesting infrared-to-visible up-conversion light-emitting lasing properties through multi-photon absorption. Here, when the optical pumping switches between circular and linear polarization, up-conversion amplified spontaneous emission (ASE) intensity exhibits large and small amplitudes, respectively, leading to a positive up-conversion ΔASE in the CsPbBr3 perovskite microrods. This observed phenomenon demonstrates that the coherent interaction between coherent light-emitting excitons is indeed established at the up-conversion ASE regime in the CsPbBr3 perovskite microrods. In addition, the positive up-conversion ΔASE indicates the orbital magnetic dipoles between coherent light-emitting excitons are conserved during up-conversion ASE action. Essentially, the up-conversion ΔASE results provide evidence that shows up-conversion ASE can be realized by the orbit−orbit polarization interaction between light-emitting excitons. Moreover, up-conversion ASE proportionally increased as the pumping fluence increased, which shows that orbit–orbit polarization interaction can be gradually enhanced between coherent light-emitting excitons by increasing pumping density in the CsPbBr3 perovskite microrods. Substantially, our studies provide a fundamental understanding of the spin alignment between coherent light-emitting excitons towards developing spin-dependent nonlinear lasing actions in metal halide perovskites.
Post-Proline Cleaving Enzymes (PPCEs): Classification, Structure, Molecular Properties, and Applications
Authors: Baharin Anis , Ting Tiew-Yik , Goh Hoe-Han
Published: May 2022

Proteases or peptidases are hydrolases that catalyze the breakdown of polypeptide chains into smaller peptide subunits. Proteases exist in all life forms, including archaea, bacteria, protozoa, insects, animals, and plants due to their vital functions in cellular processing and regulation. There are several classes of proteases in the MEROPS database based on their catalytic mechanisms. This review focuses on post-proline cleaving enzymes (PPCEs) from different peptidase families, as well as prolyl endoprotease/oligopeptidase (PEP/POP) from the serine peptidase family. To date, most PPCEs studied are of microbial and animal origins. Recently, there have been reports of plant PPCEs. The most common PEP/POP are members of the S9 family that comprise two conserved domains. The substrate-limiting β-propeller domain prevents unwanted digestion, while the α/β hydrolase catalyzes the reaction at the carboxyl-terminal of proline residues. PPCEs display preferences towards the Pro-X bonds for hydrolysis. This level of selectivity is substantial and has benefited the brewing industry, therapeutics for celiac disease by targeting proline-rich substrates, drug targets for human diseases, and proteomics analysis. Protein engineering via mutagenesis has been performed to improve heat resistance, pepsin-resistant capability, specificity, and protein turnover of PPCEs for pharmacological applications. This review aims to synthesize recent structure–function studies of PPCEs from different families of peptidases to provide insights into the molecular mechanism of prolyl cleaving activity. Despite the non-exhaustive list of PPCEs, this is the first comprehensive review to cover the biochemical properties, biological functions, and biotechnological applications of PPCEs from the diverse taxa.
Review of Particle Filters for Internal Combustion Engines
Authors: Dong Rui , Zhang Zhiqing , Ye Yanshuai , Huang Huiqiong , Cao Chao
Published: May 2022

Diesel engines have gradually become one of the main forces in the human transportation industry because of their high efficiency, good durability, and stable operation. However, compared with gasoline vehicles, the high emission of diesel vehicles forces manufacturers to introduce new pollutant control technologies. Although the particulate matter emissions of gasoline vehicles are lower than that of diesel vehicles, with the popularity of gasoline vehicles and the continuous rise of power, the impact of these particles on the environment cannot be ignored. Therefore, diesel particulate filters and gasoline particulate filters have been invented to collect the fine particles in the exhaust gas to protect the environment and meet increasingly stringent emission regulations. This paper summarizes the research progress on diesel particulate filters and gasoline particulate filters at present and comprehensively introduces the diesel particulate filter and gasoline particulate filter from the mechanism, composition, and operation processes. Additionally, the laws and regulations of various countries and the impact of gas waste particulates on the human body are described. In addition, the mechanisms of the diesel particulate filter, gasoline particulate filter, and regeneration were studied. Finally, the prospects and future directions for the development of particle filters for internal combustion engines are presented.
Non-Thermal Technologies Combined with Antimicrobial Peptides as Methods for Microbial Inactivation: A Review
Authors: Andaluz-Mejía Larissa , Ruiz-De Anda Daniela , Ozuna César
Published: May 2022

Non-thermal technologies allow for the nutritional and sensory properties of foods to be preserved, something that consumers demand. Combining their use with antimicrobial peptides (AMPs) provides potential methods for food preservation that could have advantages over the use of chemical preservatives and thermal technologies. The aim of this review was to discuss the advances in the application of non-thermal technologies in combination with AMPs as a method for microbial inactivation. Published papers reporting studies on the combined use of power ultrasound (US), pulsed electrical fields (PEF), and high hydrostatic pressure (HHP) with AMPs were reviewed. All three technologies show a possibility of being combined with AMPs, generally demonstrating higher efficiency than the application of US, PEF, HHP, and AMPs separately. The most studied AMP used in combination with the three technologies was nisin, probably due to the fact that it is already officially regulated. However, the combination of these non-thermal technologies with other AMPs also shows promising results for microbial inactivation, as does the combination of AMPs with other novel non-thermal technologies. The effectiveness of the combined treatment depends on several factors; in particular, the characteristics of the food matrix, the conditions of the non-thermal treatment, and the conditions of AMP application.
Particle Residence Time Distribution in a Concurrent Multiphase Flow Reactor: Experiments and Euler-Lagrange Simulations
Authors: Unger Laura , Gómez Bonilla Juan Sebastián , dos Santos Dyrney Araújo , Bück Andreas
Published: May 2022

The present work focuses on investigating the residence time behavior of microparticles in a concurrent downer reactor through experiments and numerical simulations. For the numerical simulations, a three-dimensional multiphase model was developed using the Euler-Lagrange approach. The experiments were performed in a 0.8 m-long steel reactor with gravitational particle injection. The effects of different operating conditions, e.g., the sheath gas velocity on the particle residence time distribution were assessed. An increase in the sheath gas flow rate led to a decrease in the peak residence time, although the maximum residence time increased. Regarding the lowest sheath gas flow rate, the particles’ peak residence time was twice as high compared to the peak residence time within the highest flow rate. The particles’ residence time curves presented a broad distribution coinciding with the size distribution of the powder. The numerical results agreed with the experimental data; thus, this study presents a numerical model for predicting the particle residence time behavior in a concurrent downer reactor. Furthermore, the numerical simulations contributed to a better understanding of the particle residence time behavior inside a concurrent downer reactor which is essential for optimizing thermal rounding processes. Dimensionless correlations for the observed effects are developed.
Effect of Spray Parameters on Electrical Characteristics of Printed Layer by Morphological Study
Authors: Kim Gye Hyeon , Shin Eun Ae , Jung Je Young , Lee Jun Young , Lee Chang Kee
Published: May 2022

Products are manufactured as printed electronics through electro-conductive ink having properties suitable for flexible substrates. As printing process conditions affect the quality of the electronic properties of the final devices, it is essential to understand how the parameters of each process affect print quality. Spray printing, one of several printing processes, suits flexible large-area substrates and continuous processes with a uniform layer for electro-conductive aqueous ink. This study adopted the spray printing process for cellulose nanofiber (CNF)/carbon nanotube (CNT) composite conductive printing. Five spray parameters (nozzle diameter, spray speed, amount of sprayed ink, distance of nozzle to substrate, and nozzle pressure) were chosen to investigate the effects between process parameters and electrical properties relating to the morphology of the printing products. This study observed the controlling morphology through parameter adjustment and confirmed how it affects the final electrical conductivity. It means that the quality of the electronic properties can be modified by adjusting several spray process parameters.
sEMG and Vibration System Monitoring for Differential Diagnosis in Temporomandibular Joint Disorders
Authors: Kulesa-Mrowiecka Małgorzata , Barański Robert , Kłaczyński Maciej
Published: May 2022

The stomatognathic system represents an important element of human physiology, constituting a part of the digestive, respiratory, and sensory systems. One of the signs of temporomandibular joint disorders (TMD) can be the formation of vibroacoustic and electromyographic (sEMG) phenomena. The aim of the study was to evaluate the effectiveness of temporomandibular joint rehabilitation in patients suffering from locking of the temporomandibular joint (TMJ) articular disc by analysis of vibrations, sEMG registration of masseter muscles, and hypertension of masticatory muscles. In this paper, a new system for the diagnosis of TMD during rehabilitation is proposed, based on the use of vibration and sEMG signals. The operation of the system was illustrated in a case study, a 27-year-old woman with articular dysfunction of the TMJ. The first results of TMD diagnostics using the k-nearest neighbors method are also presented on a group of fifteen people (ten women and five men). Vibroacoustic registration of temporomandibular joints, sEMG registration of masseter muscles, and functional manual analysis of the TMJ were simultaneously assessed before employing splint therapy with stomatognathic physiotherapy. Analysis of vibrations with the monitoring of sEMG in dysfunctions of the TMJ can lead to improve differential diagnosis and can be an objective way of monitoring the rehabilitation process of TMD.
A New Self-Calibration and Compensation Method for Installation Errors of Uniaxial Rotation Module Inertial Navigation System
Authors: Niu Meng , Ma Hongyu , Sun Xinglin , Huang Tiantian , Song Kaichen
Published: May 2022

Calibration and compensation techniques are essential to improve the accuracy of the strap-down inertial navigation system. Especially for the new uniaxial rotation module inertial navigation system (URMINS), replacing faulty uniaxial rotation modules introduces installation errors between modules and reduces navigation accuracy. Therefore, it is necessary to calibrate these systems effectively and compensate for the installation error between modules. This paper proposes a new self-calibration and compensation method for installation errors without additional information and equipment. Using the attitude, velocity, and position differences between the two sets of navigation information output from URMINS as measurements, a Kalman filter is constructed and the installation error is estimated. After URMINS is compensated for the installation error, the average of the demodulated redundant information is taken to calculate the carrier’s navigation information. The simulation results show that the proposed method can effectively assess the installation error between modules with an estimation accuracy better than 5”. Experimental results for static navigation show that the accuracy of heading angle and positioning can be improved by 73.12% and 81.19% after the URMINS has compensated for the estimated installation errors. Simulation and experimental results further validate the effectiveness of the proposed self-calibration and compensation method.
Real-Time Vehicle Classification and Tracking Using a Transfer Learning-Improved Deep Learning Network
Authors: Neupane Bipul , Horanont Teerayut , Aryal Jagannath
Published: May 2022

Accurate vehicle classification and tracking are increasingly important subjects for intelligent transport systems (ITSs) and for planning that utilizes precise location intelligence. Deep learning (DL) and computer vision are intelligent methods; however, accurate real-time classification and tracking come with problems. We tackle three prominent problems (P1, P2, and P3): the need for a large training dataset (P1), the domain-shift problem (P2), and coupling a real-time multi-vehicle tracking algorithm with DL (P3). To address P1, we created a training dataset of nearly 30,000 samples from existing cameras with seven classes of vehicles. To tackle P2, we trained and applied transfer learning-based fine-tuning on several state-of-the-art YOLO (You Only Look Once) networks. For P3, we propose a multi-vehicle tracking algorithm that obtains the per-lane count, classification, and speed of vehicles in real time. The experiments showed that accuracy doubled after fine-tuning (71% vs. up to 30%). Based on a comparison of four YOLO networks, coupling the YOLOv5-large network to our tracking algorithm provided a trade-off between overall accuracy (95% vs. up to 90%), loss (0.033 vs. up to 0.036), and model size (91.6 MB vs. up to 120.6 MB). The implications of these results are in spatial information management and sensing for intelligent transport planning.
Floating Wetlands for Sustainable Drainage Wastewater Treatment
Authors: Gabr Mohamed Elsayed , Salem Madleen , Mahanna Hani , Mossad Mohamed
Published: May 2022

The preservation of water resources in modern urbanized society is a major concern. In this study, a floating constructed wetland (FWT) pilot plant was designed and constructed for the treatment of a polluted wastewater drain. A series of experiments were run continuously for a year in pilot-scale FWTs in a semi-arid area located in Egypt’s Delta. Four aquatic plant species (Eichhornia, Ceratophyllum, Pistia stratiotes, and Nymphaea lotus) were used to assess the performance of FWTs for pollutant removals, such as biochemical oxygen demand (BOD), chemical oxygen demand (COD), total suspended solids (TSS), total nitrogen (TN), total phosphorus (TP), electrical conductivity (EC), and total dissolved solids (TDS), from drainage wastewater to reuse the treated effluent in irrigation practices. The FWT systems were fed drainage tainted water on a weekly basis, and the concentrations and removal efficiency were assessed in the experiments. The average reduction in BOD, COD, TSS, TDS, TN, EC, and TP were 76–86%, 61–80%, 87–95%, 36.6–44.1%, 70–97%, 37–44%, and 83–96%, respectively. ANOVA with Post-HOC t-tests show that the Eichhornia, Pistia stratiotes, and Nymphaea lotus have the highest BOD and COD removal performance, whereas Pistia stratiotes and Nymphaea lotus have the highest TN and TP removal performance. In all cases, the Nymphaea lotus performed well in terms of pollutant removal. In addition, a design procedure for a FWT systems is presented. For wastewater treatment, FWT systems have proven to be a low-cost, long-term option.
Development of a 96-Well Electrophilic Allergen Screening Assay for Skin Sensitization Using a Measurement Science Approach
Authors: Petersen Elijah J. , Uhl Richard , Toman Blaza , Elliott John T. , Strickland Judy , Truax James , Gordon John
Published: May 2022

The Electrophilic Allergen Screening Assay (EASA) has emerged as a promising in chemico method to detect the first key event in the adverse outcome pathway (AOP) for skin sensitization. This assay functions by assessing the depletion of one of two probe molecules (4-nitrobenzenethiol (NBT) and pyridoxylamine (PDA)) in the presence of a test compound (TC). The initial development of EASA utilized a cuvette format resulting in multiple measurement challenges such as low throughput and the inability to include adequate control measurements. In this study, we describe the redesign of EASA into a 96-well plate format that incorporates in-process control measurements to quantify key sources of variability each time the assay is run. The data from the analysis of 67 TCs using the 96-well format had 77% concordance with animal data from the local lymph node assay (LLNA), a result consistent with that for the direct peptide reactivity assay (DPRA), an OECD test guideline (442C) protein binding assay. Overall, the measurement science approach described here provides steps during assay development that can be taken to increase confidence of in chemico assays by attempting to fully characterize the sources of variability and potential biases and incorporate in-process control measurements into the assay.
Towards Reference Values for Malondialdehyde on Exhaled Breath Condensate: A Systematic Literature Review and Meta-Analysis
Authors: Turcu Veronica , Wild Pascal , Hemmendinger Maud , Sauvain Jean-Jacques , Bergamaschi Enrico , Hopf Nancy B. , Canu Irina Guseva
Published: May 2022

Many pathological conditions and certain airway exposures are associated with oxidative stress (OS). Malondialdehyde (MDA) is an end-product of the oxidation of lipids in our cells and is present in all biological matrices including exhaled breath condensate (EBC). To use MDA as a biomarker of OS in EBC, a reference interval should be defined. Thus, we sought to summarize reference values reported in healthy adult populations by performing a systematic review and meta-analysis using a standardized protocol registered in PROSPERO (CRD42020146623). Articles were retrieved from four major databases and 25 studies with 28 subgroups were included. Defining the distribution of MDA measured in reference populations with a detection combined with a separation technique still represents a challenge due to the low number of studies available, different analytical methods used, and questionable methodological qualities of many studies. The most salient methodological drawbacks have been in data collection and reporting of methods and study results by the researchers. The lack of compliance with the recommendations of the European Respiratory Society and American Thoracic Society was the major limitation in the current research involving EBC. Consequently, we were unable to establish a reference interval for MDA in EBC.