bluuebunny/crossref_metadata_2025 huggingface dataset:
Biological Regulatory Network (BRN) Analysis and Molecular Docking Simulations to Probe the Modulation of IP3R Mediated Ca2+ Signaling in Cancer
Authors: Ismatullah Humaira , Jabeen Ishrat , Saeed Muhammad Tariq
Published: December 2020

Inositol trisphosphate receptor (IP3R) mediated Ca+2 signaling is essential in determining the cell fate by regulating numerous cellular processes, including cell division and cell death. Despite extensive studies about the characterization of IP3R in cancer, the underlying molecular mechanism initiating the cell proliferation and apoptosis remained enigmatic. Moreover, in cancer, the modulation of IP3R in downstream signaling pathways, which control oncogenesis and cancer progression, is not well characterized. Here, we constructed a biological regulatory network (BRN), and describe the remodeling of IP3R mediated Ca2+ signaling as a central key that controls the cellular processes in cancer. Moreover, we summarize how the inhibition of IP3R affects the deregulated cell proliferation and cell death in cancer cells and results in the initiation of pro-survival responses in resistance of cell death in normal cells. Further, we also investigated the role of stereo-specificity of IP3 molecule and its analogs in binding with the IP3 receptor. Molecular docking simulations showed that the hydroxyl group at R6 position along with the phosphate group at R5 position in ‘R’ conformation is more favorable for IP3 interactions. Additionally, Arg-266 and Arg-510 showed π–π and hydrogen bond interactions and Ser-278 forms hydrogen bond interactions with the IP3 binding site. Thus, they are identified as crucial for the binding of antagonists.
Evaluation of a Prehospital Rotation by Senior Residents: A Web-Based Survey
Authors: Suppan Laurent , Chan Michèle , Gartner Birgit , Regard Simon , Campana Mathieu , Chatellard Ghislaine , Cottet Philippe , Larribau Robert , Sarasin François Pierre , Niquille Marc
Published: December 2020

The added value of prehospital emergency medicine is usually assessed by measuring patient-centered outcomes. Prehospital rotations might however also help senior residents acquire specific skills and knowledge. To assess the perceived added value of the prehospital rotation in comparison with other rotations, we analyzed web-based questionnaires sent between September 2011 and August 2020 to senior residents who had just completed a prehospital rotation. The primary outcome was the perceived benefit of the prehospital rotation in comparison with other rotations regarding technical and non-technical skills. Secondary outcomes included resident satisfaction regarding the prehospital rotation and regarding supervision. A pre-specified subgroup analysis was performed to search for differences according to the participants’ service of origin (anesthesiology, emergency medicine, or internal medicine). The completion rate was of 71.5% (113/158), and 91 surveys were analyzed. Most senior residents found the prehospital rotation either more beneficial or much more beneficial than other rotations regarding the acquisition of technical and non-technical skills. Anesthesiology residents reported less benefits than other residents regarding pharmacological knowledge acquisition and confidence as to their ability to manage emergency situations. Simulation studies should now be carried out to confirm these findings.
Development and Investigation of a New Model Explaining Job Performance and Uncertainty among Nurses and Physicians
Authors: Olsen Espen , Mikkelsen Aslaug
Published: December 2020

The purpose of this paper is to develop and investigate a new theoretical model explaining variance in job performance and uncertainty among nurses and physicians. The study adopted a cross-sectional survey. Data was collected from 2946 nurses and 556 physicians employed at four public hospitals in Norway. We analysed data using descriptive statistics, correlations, Cronbach’s alpha, confirmatory factor analyses and structural equation modelling. To explain job performance and uncertainty, two sets of explanatory variables were used: first, satisfactions of three psychological needs—namely autonomy, social support and competence development—and second, employee perceptions of hospital management quality (HMQ) and local leadership quality (LLQ). The results supported the theoretical model among nurses and physicians; (1) HMQ was positively associated with LLQ; (2) LLQ was positively associated with psychological needs; (3) the majority of psychological needs were positively associated with job performance and negatively associated with uncertainty, but more of these relations were significant among nurses than physicians. The results suggest that job performance and uncertainty among nurses and physicians can be improved by helping personnel meet their psychological needs. Improving job design and staff involvement will be important to strengthen need satisfaction. Results suggest enhancement of HMQ and LLQ will be positively related to need satisfaction among nurses and physicians and will strengthen job performance and reduce uncertainty.
Food Insecurity Is Associated with Depression among a Vulnerable Workforce: Early Care and Education Workers
Authors: Loh Ivory H. , Oddo Vanessa M. , Otten Jennifer
Published: December 2020

Objective: We aimed to explore the association between food insecurity and depression among early care and education (ECE) workers, a vulnerable population often working in precarious conditions. Design: We utilized cross-sectional data from a study exploring the effects of wage on ECE centers. Participants were enrolled between August 2017 and December 2018. Food insecurity was measured using the validated six-item U.S. Household Food Security Survey Module and participants were categorized as food secure (score = 0–1), low food security (score = 2–4), and very low food security (score = 5–6). Depression (defined as a score ≥ 16) was measured using the 20-item Center for Epidemiologic Studies Depression Scale-Revised. We employed a logistic regression model to examine the relationship between food insecurity and depression. All models controlled for marital status, nativity, race/ethnicity, number of children in the household, job title, weekly hours of work, education, income, and study site. Setting: Participants were from Seattle (40%) and South King County (26%), Washington, and Austin, Texas (34%). Participants: Participants included 313 ECE workers from 49 ECE centers. Results: A majority of participants were female, non-Hispanic White, born in the U.S., and did not have children. Compared to being food secure, very low and low food insecurities were associated with a 4.95 (95% confidence interval (CI): 2.29, 10.67) and 2.69 (95% CI: 1.29, 5.63) higher odds of depression, respectively. Conclusions: Policies and center-level interventions that address both food insecurity and depression may be warranted, in order to protect and improve the health of this valuable, yet vulnerable, segment of the U.S. workforce.
Postural Stability in Athletes during Special Hurdle Tests without a Definite Dominant Leg
Authors: Wojciechowska-Maszkowska Bożena , Marcinów Ryszard , Iskra Janusz , Tataruch Rafał
Published: December 2020

The purpose of this study was to analyze the body balance control of people walking and hurdling with or without a specific dominant leg in a monopodal position. This study involved 28 physical education students. The center of pressure (COP) was measured with a force plate under four conditions: single-leg standing (right and left) with eyes open and two upper limb positions (the arms were positioned in either a specific hurdle technique manner or alongside the body). A repeated measures analysis of variance (ANOVA) was conducted separately for five parameters of the COP in the medial-lateral (ML) and anterior-posterior (AP) directions under the four conditions. In the single-leg position, athletes without a dominant lower leg had better body balance than those with a dominant lower leg. The position of the upper limbs influenced the body position when hurdling. Accepting the correct position of the upper limbs helped to maintain balance (when overcoming hurdles). In hurdles, the position of the upper limbs should be improved to maintain postural stability and save this function for more demanding postural tasks.
Availability of Essential Medicines in a Country in Conflict: A Quantitative Insight from Yemen
Authors: Mohamed Ibrahim Mohamed Izham , Alshakka Mohammed , Al-abd Nazeh , Bahattab Awsan , Badulla Wafa
Published: December 2020

Background: Medicine and medical supplies are often in short supply in countries suffering from the scourge of conflict. Effective medicine supply policies are lacking in many low- and middle-income countries (LMICs), particularly during conflict. This study aimed to assess the availability of essential medicines in both the public and private healthcare sectors. Methods: The study was conducted by administering a survey from November 2017 to February 2018 using the World Health Organization/Health Action International (WHO/HAI) guidelines and methodology. Thirty healthcare facilities in thirteen districts from three governorates in Yemen were included in the assessment of thirty essential medicines. The results were reported as frequencies and percentages of outlets with available medicines on the day of data collection. Results: A set of 30 vital and essential medicines were selected from the list of essential medicines that are used in healthcare centers in Yemen to treat prevalent diseases. In general, only 52.8% of the selected medicines were available in public and private healthcare settings. The distribution and availability of medicines in the three governorates were approximately equal. The availability of medicines was better in the private healthcare settings, specifically 73.3% in private hospitals and approximately 79.7% in private pharmacies. Conclusions: The availability of essential medicines during this state of conflict in three governorates in Yemen is low, in both public and private hospitals and healthcare centers. Many of the medications that were not available are used to treat chronic illnesses.
Sociodemographic Predictors of Changes in Physical Activity, Screen Time, and Sleep among Toddlers and Preschoolers in Chile during the COVID-19 Pandemic
Authors: Aguilar-Farias Nicolas , Toledo-Vargas Marcelo , Miranda-Marquez Sebastian , Cortinez-O'Ryan Andrea , Cristi-Montero Carlos , Rodriguez-Rodriguez Fernando , Martino-Fuentealba Pia , Okely Anthony D. , del Pozo Cruz Borja
Published: December 2020

The aim was to examine the sociodemographic predictors associated with changes in movement behaviors (physical activity, screen time, and sleep) among toddlers and preschoolers during the early stages of the coronavirus disease 2019 pandemic in Chile. Caregivers of 1- to 5-year-old children completed an online survey between 30 March and 27 April 2020. Information about the child’s movement behaviors before (retrospectively) and during the pandemic, as well as family characteristics were reported. In total, 3157 participants provided complete data (mean children age: 3.1 ± 1.38 years). During early stages of the pandemic, time spent in physical activity decreased, recreational screen time and sleep duration increased, and sleep quality declined. Toddlers and preschoolers with space to play at home and living in rural areas experienced an attenuated impact of the pandemic restrictions on their physical activity levels, screen time, and sleep quality. Older children, those whose caregivers were aged ≥35–<45 years and had a higher educational level, and those living in apartments had greater changes, mainly a decrease in total physical activity and increase in screen time. This study has shown the significant impact of the pandemic restrictions on movement behaviors in toddlers and preschoolers in Chile.
Ammonia Volatilization and Greenhouse Gases Emissions during Vermicomposting with Animal Manures and Biochar to Enhance Sustainability
Authors: Raza Syed Turab , Tang Jia Liang , Ali Zulfiqar , Yao Zhiyuan , Bah Hamidou , Iqbal Hassan , Ren Xiao
Published: December 2020

There is a huge potential for nutrient recovery from organic waste materials for soil fertility restoration as well as negative environmental emission mitigation. Previous research has found vermicomposting the optimal choice for converting organic waste into beneficial organic fertilizer while reducing reactive N loss. However, a great deal of the processes of greenhouse gases (GHG) and ammonia volatilization during vermicomposting are not well-documented. A field vermicomposting experiment was conducted by deploying earthworms (Eisenia fetida) with three types of agricultural by-products—namely, cow manure (VCM), pig manure (VPM), and biochar (VBC)—and crop (maize) residues compared with traditional composting (COM) without earthworms in the Sichuan Basin, China. Results showed that vermicomposting caused a decrease in electrical conductivity (EC) and total organic carbon (TOC) while increasing total nitrogen (TN). The greatest TN increase was found with VCM. The cumulative NH3 volatilization in COM, VCM, VPM, and VBC during experimental duration was 9.00, 8.02, 15.16, and 8.91 kg N ha−1, respectively. The cumulative CO2 emissions in COM, VCM, VPM, and VBC were 2369, 2814, 3435, and 2984 (g·C·m−2), while for CH4, they were 0.36, 0.28, 4.07, and 0.19 (g·C·m−2) and, for N2O, they were 0.12, 0.06, 0.76, and 0.04 (g·N m−2), respectively. Lower emissions of N2O, CH4, and NH3 were observed in VBC. We concluded that earthworms, as ecological engineers, enhanced reactive nutrients and reduced ammonia volatilization during vermicomposting in our test system. Overall, vermicomposting is proposed as an eco-friendly, sustainable technique that helps to reduce environmental impacts and associated health risks.
Sex and Exposure to Postnatal Chlorpyrifos Influence the Epigenetics of Feeding-Related Genes in a Transgenic APOE Mouse Model: Long-Term Implications on Body Weight after a High-Fat Diet
Authors: Guardia-Escote Laia , Blanco Jordi , Basaure Pia , Biosca-Brull Judit , Verkaik-Schakel Rikst Nynke , Cabré Maria , Peris-Sampedro Fiona , Pérez-Fernández Cristian , Sánchez-Santed Fernando , Plösch Torsten , Domingo José L. , Colomina Maria Teresa
Published: December 2020

Developmental exposure to toxicants and diet can interact with an individual’s genetics and produce long-lasting metabolic adaptations. The different isoforms of the apolipoprotein E (APOE) are an important source of variability in metabolic disorders and influence the response to the pesticide chlorpyrifos (CPF). We aimed to study the epigenetic regulation on feeding control genes and the influence of postnatal CPF exposure, APOE genotype, and sex, and how these modifications impact on the metabolic response to a high-fat diet (HFD). Both male and female apoE3- and apoE4-TR mice were exposed to CPF on postnatal days 10–15. The DNA methylation pattern of proopiomelanocortin, neuropeptide Y, leptin receptor, and insulin-like growth factor 2 was studied in the hypothalamus. At adulthood, the mice were given a HFD for eight weeks. The results highlight the importance of sex in the epigenetic regulation and the implication of CPF treatment and APOE genotype. The body weight progression exhibited sex-dimorphic differences, apoE4-TR males being the most susceptible to the effects induced by CPF and HFD. Overall, these results underscore the pivotal role of sex, APOE genotype, and developmental exposure to CPF on subsequent metabolic disturbances later in life and show that sex is a key variable in epigenetic regulation.
Normative Hand Strength of Healthcare Industry Workers in Central Taiwan
Authors: Lo Victor Ei-Wen , Chao Shu-Min , Tu Hsin-Hung
Published: December 2020

Objectives: The purpose of this study is to establish the norms of hand grip strength in the healthcare industry in Taiwan and propose models to predict the strength of hand movement by regression with demographic and anthropometric factors. Methods: This is a cross-sectional study with a stratified convenience sample of workers in healthcare service industries in central Taiwan. Three hundred twenty-nine healthy subjects were recruited. Strength of different hand movement were tested three times in both hands and rests were given between tests. Results: Female strength of these hand movement was 59.1% to 73.0% that in males (p < 0.001). In general, the hand strength of male workers in the healthcare industry was less than that of male workers in the manufacturing industry (p < 0.001). In the prediction model, sex and weight played important roles in predicting hand strength. Conclusions: The norms of different types of hand strength was investigated the first time in workers in the healthcare industry in Taiwan. The tasks performed by healthcare personnel vary widely, and this variable should be considered in a future prediction model.
Investigating the Dynamics of China’s Green Building Policy Development from 1986 to 2019
Authors: Wu Zezhou , He Qiufeng , Yang Kaijie , Zhang Jinming , Xu Kexi
Published: December 2020

China has enacted numerous green building policies (GBPs) to promote green building (GB) development in the past decades. Investigating the evolution characteristics of China’s GBPs is significant for the future optimization of the GBP system. However, few studies on this topic have been conducted. To bridge this research gap, this paper adopted the methods of bibliometric analysis and text mining to probe the dynamic evolution of the GBPs in China. Firstly, a total 199 collected policies from 1986 to 2019 were grouped into five stages according to the Five-Year Plan. Then, the topics emphasized in different stages and the cooperative relationships among policymaking agencies were discovered by mapping and visualizing the co-word network and co-author network. Based on the derived results, an in-depth discussion was further conducted from five aspects: targets, objects, instruments, GB performance indicators, and the collaboration structure of policymaking agencies. It was revealed that the topics of GBPs evolved from macro to specific, and the types of policy targets, objects, instruments, and GB performance indicators evolved from few to multiple. Additionally, the collaboration structure of policymaking agencies went from dispersive to centralized. This study sheds lights on the dynamic evolution of China’s GBPs and provides valuable references for other countries in need.
Are Yellow Sticky Cards and Light Traps Effective on Tea Green Leafhoppers and Their Predators in Chinese Tea Plantations?
Authors: Shi Longqing , He Haifang , Yang Guang , Huang Huoshui , Vasseur Liette , You Minsheng
Published: December 2020

In Chinese tea plantations, yellow sticky cards and light traps are increasingly used to control insect pests, especially the tea green leafhopper Empoasca onukii. In this study, a 16-week open-field experiment with daily weather monitoring was designed to test the responses of tea green leafhopper, parasitoids and spiders to yellow sticky cards and three light traps with different wavelengths (covered with sticky cards). An exclosure experiment was also designed to further test the influence of the three light systems (without sticky card) on the same species. The results showed that all three light emitting diode (LED) light traps (white, green and yellow) and yellow sticky cards attracted many more E. onukii male adults than females during the course of the open field experiment, with less than 25% of trapped adults being females. Parasitoids and spiders were also attracted by these systems. Weather variables, especially rainfall, influenced the trapping efficiency. In the exclosure experiment, the population of leafhoppers in the yellow sticky card treatment did not decline significantly, but the number of spiders significantly decreased. The green and white light treatments without sticky cards showed a significant control of E. onukii and no obvious harm to spiders. These results suggest that yellow sticky cards and light traps have limited capacity to control tea green leafhoppers. However, light, especially green light, may be a promising population control measure for tea green leafhoppers, not as killing agents in the traps, but rather as a behavioral control system.
Fast, Cheap, and Scalable Magnetic Tracker with an Array of Magnetoresistors
Authors: Biancalana Valerio , Cecchi Roberto , Chessa Piero , Bevilacqua Giuseppe , Dancheva Yordanka , Vigilante Antonio
Published: December 2020

We present the hardware of a cheap multi-sensor magnetometric setup, where a relatively large set of magnetic field components is measured in several positions by calibrated magnetoresistive detectors. The setup is developed to map the (inhomogeneous) field generated by a known magnetic source, which is measured and then discerned from the background (homogeneous) geomagnetic field. The data output from this hardware can be successfully and reliably used to retrieve the position and orientation of the magnetic source with respect to the sensor frame, together with the orientation of the frame with respect to the environmental field. Possible applications of the setup are briefly discussed, and a synthetic description of the methods of data elaboration and analysis is provided.
Analysis of Corneal Distortion after Myopic PRK
Authors: Lanza Michele , De Rosa Luigi , Sbordone Sandro , Boccia Rosa , Gironi Carnevale Ugo Antonello , Simonelli Francesca
Published: December 2020

The purpose of the study is to evaluate the corneal biomechanical properties (CBP) and their behaviors after myopic refractive surgery both with Ocular Response Analyzer (ORA) and Corvis ST (CST). This retrospective study included 145 eyes of 145 patients with a mean age of 33.13 ± 9.24 years, who underwent myopic photorefractive keratectomy (PRK) for a refractive defect, measured as spherical equivalent, of mean −4.69 ± 2.04 D and have been evaluated before surgery and at 1, 3 and 6 months follow-up. Corneal hysteresis (CH) and corneal resistance factor (CRF) values significantly decreased after 1 month and remained statistically stable during further follow-ups. CST parameters had a different evolution: only second applanation time (AT2) differences showed a significant variation after 1 month that did not statistically change over time. Highest concavity deformation amplitude (HCDA), highest concavity peak distance (HCPD), first applanation time (AT1) and velocity (AV1) showed continuous significant differences both after 3 and after 6 months. This study suggests that after central surface ablation surgery, such as myopic PRK, corneal shape is remodeling, and its deformation parameters are going to change even at 6 months follow-up. This indicates that it should be important to evaluate refractive surgery patients during a longer follow-up because this could allow earlier diagnosis and better management of late-onset complications.
Thromboembolic and Bleeding Risk in Atrial Fibrillation Patients with Chronic Kidney Disease: Role of Anticoagulation Therapy
Authors: Magnocavallo Michele , Bellasi Antonio , Mariani Marco Valerio , Fusaro Maria , Ravera Maura , Paoletti Ernesto , Di Iorio Biagio , Barbera Vincenzo , Della Rocca Domenico Giovanni , Palumbo Roberto , Severino Paolo , Lavalle Carlo , Di Lullo Luca
Published: December 2020

Atrial fibrillation (AF) and chronic kidney disease (CKD) are strictly related; several independent risk factors of AF are often frequent in CKD patients. AF prevalence is very common among these patients, ranging between 15% and 20% in advanced stages of CKD. Moreover, the results of several studies showed that AF patients with end stage renal disease (ESRD) have a higher mortality rate than patients with preserved renal function due to an increased incidence of stroke and an unpredicted elevated hemorrhagic risk. Direct oral anticoagulants (DOACs) are currently contraindicated in patients with ESRD and vitamin K antagonists (VKAs), remaining the only drugs allowed, although they show numerous critical issues such as a narrow therapeutic window, increased tissue calcification and an unfavorable risk/benefit ratio with low stroke prevention effect and augmented risk of major bleeding. The purpose of this review is to shed light on the applications of DOAC therapy in CKD patients, especially in ESRD patients.
Current and New Perspectives in the Diagnosis of Blastomycosis and Histoplasmosis
Authors: Linder Kathleen A. , Kauffman Carol A.
Published: December 2020

The diagnosis of blastomycosis and histoplasmosis can be difficult for clinicians who rarely see infections caused by these environmentally restricted dimorphic fungi. Historically, the diagnosis of blastomycosis has been established by culture and sometimes by histopathologic identification. Currently, antigen detection in urine and serum has been shown to aid in the rapid diagnosis of blastomycosis, and newer antibody assays are likely to contribute to our diagnostic capability in the near future. The gold standard for the diagnosis of histoplasmosis has been culture of the organism from involved tissues, aided in some patients by histopathological verification of the typical yeast forms in tissues. Antigen detection has contributed greatly to the ability of clinicians to rapidly establish the diagnosis of histoplasmosis, especially in severely ill and immunocompromised patients, and antibody testing for Histoplasma capsulatum provides important adjunctive diagnostic capability for several forms of both acute and chronic histoplasmosis. For both of these endemic mycoses, novel molecular tests are under active investigation, but remain available in only a few reference laboratories. In this review, we provide a synopsis of diagnostic test options that aid in establishing whether a patient has blastomycosis or histoplasmosis.
COVID-19 Outbreak and CO2 Emissions: Macro-Financial Linkages
Authors: Chevallier Julien
Published: December 2020

In the Dynamic Conditional Correlation with Mixed Data Sampling (DCC-MIDAS) framework, we scrutinize the correlations between the macro-financial environment and CO2 emissions in the aftermath of the COVID-19 diffusion. The main original idea is that the economy’s lock-down will alleviate part of the greenhouse gases’ burden that human activity induces on the environment. We capture the time-varying correlations between U.S. COVID-19 confirmed cases, deaths, and recovered cases that were recorded by the Johns Hopkins Coronavirus Center, on the one hand; U.S. Total Industrial Production Index and Total Fossil Fuels CO2 emissions from the U.S. Energy Information Administration on the other hand. High-frequency data for U.S. stock markets are included with five-minute realized volatility from the Oxford-Man Institute of Quantitative Finance. The DCC-MIDAS approach indicates that COVID-19 confirmed cases and deaths negatively influence the macro-financial variables and CO2 emissions. We quantify the time-varying correlations of CO2 emissions with either COVID-19 confirmed cases or COVID-19 deaths to sharply decrease by −15% to −30%. The main takeaway is that we track correlations and reveal a recessionary outlook against the background of the pandemic.
Lashing Force Prediction Model with Multimodal Deep Learning and AutoML for Stowage Planning Automation in Containerships
Authors: Lee Chaemin , Lee Mun Keong , Shin Jae Young
Published: December 2020

The calculation of lashing forces on containerships is one of the most important aspects in terms of cargo safety, as well as slot utilization, especially for large containerships such as more than 10,000 TEU (Twenty-foot Equivalent Unit). It is a challenge for stowage planners when large containerships are in the last port of region because mostly the ship is full and the stacks on deck are very high. However, the lashing force calculation is highly dependent on the Classification society (Class) where the ship is certified; its formula is not published and it is different per each Class (e.g., Lloyd, DNVGL, ABS, BV, and so on). Therefore, the lashing result calculation can only be verified by the Class certified by the Onboard Stability Program (OSP). To ensure that the lashing result is compiled in the stowage plan submitted, stowage planners in office must rely on the same copy of OSP. This study introduces the model to extract the features and to predict the lashing forces with machine learning without explicit calculation of lashing force. The multimodal deep learning with the ANN, CNN and RNN, and AutoML approach is proposed for the machine learning model. The trained model is able to predict the lashing force result and its result is close to the result from its Class.
Mechanical Properties and Epoxy Resin Infiltration Behavior of Carbon-Nanotube-Fiber-Based Single-Fiber Composites
Authors: Shin Jongseon , Lee Kyunbae , Jung Yeonsu , Park Byeongjin , Yang Seung Jae , Kim Taehoon , Lee Sang Bok
Published: December 2020

Carbon nanotube fiber (CNTF), prepared by the direct-spinning method, has several nanopores, and the infiltration behavior of resins into these nanopores could influence the mechanical properties of CNTF-based composites. In this work, we investigated the infiltration behavior of resin into the nanopores of the CNTFs and mechanical properties of the CNTF-based single-fiber composites using six epoxy resins with varying viscosities. Epoxy resins can be easily infiltrated into the nanopores of the CNTF; however, pores appear when a resin with significantly high or low viscosity is used in the preparation process of the composites. All the composite fibers exhibit lower load-at-break value compared to as-densified CNTF, which is an unexpected phenomenon. It is speculated that the bundle structure of the CNTF can undergo changes due to the high affinity between the epoxy and CNTF. As composite fibers containing pores exhibit an even lower load-at-break value, the removal of pores by the defoaming process is essential to enhance the mechanical properties of the composite fibers.
Generation of 3D Turbine Blades for Automotive Organic Rankine Cycles: Mathematical and Computational Perspectives
Authors: Alshammari Fuhaid , Pesyridis Apostolos , Elashmawy Mohamed
Published: December 2020

Organic Rankine cycle technology is gaining increasing interest as one of potent future waste heat recovery potential from internal combustion engines. The turbine is the component where power production takes place. Therefore, careful attention to the turbine design through mathematical and numerical simulations is required. As the rotor is the main component of the turbine, the generation of the 3D shape of the rotor blades and stator vanes is of great importance. Although several types of commercial software have been developed, such types are still expensive and time-consuming. In this study, detailed mathematical modelling was presented. To account for real gas properties, REFPROP software was used. Moreover, a detailed 3D CFD numerical analysis was presented to examine the nature of the flow after generating the 3D shapes of the turbine. Moreover, finite element analysis was performed using various types of materials to obtain best-fit material for the current application. As the turbine is part of a larger system (i.e., ORC system), the effects of its performance on the whole ORC system were discussed. The results showed that the flow was smooth with no recirculation at the design point except at the last part of the suction surface where strong vortices were noticed. Despite the strong vortices, the mathematical model proved to be an effective and fast tool for the generation of the 3D shapes of turbine blades and vanes. The deviations between the 1D mean-line and 3D CFD in turbine efficiency and power output were 2.28% and 5.10%, respectively.
Effects of Resveratrol on Thymic Stromal Lymphopoietin Expression in Mast Cells
Authors: Moon Phil-Dong , Han Na-Ra , Lee Jin Soo , Jee Hyun-Woo , Kim Ji-Hyeon , Kim Hyung-Min , Jeong Hyun-Ja
Published: December 2020

Background and objectives: Cytokine thymic stromal lymphopoietin (TSLP) plays a pivotal role in the pathogenesis of atopic diseases such as atopic dermatitis, allergic rhinitis, and asthma. Resveratrol (RSV) exerts various pharmacological effects such as antioxidant, anti-inflammatory, neuroprotective, and anticancer. Although, it has been verified the beneficial effects of RSV on various subjects, the effect of RSV on thymic stromal lymphopoietin (TSLP) regulation has not been elucidated. Materials and Methods: Here, we examined how RSV regulates TSLP in HMC-1 cells. Enzyme-linked immunosorbent assay, real-time polymerase chain reaction, Western blotting, and calcium assay were performed to evaluate the effect of RSV. Results: TSLP production and mRNA expression were reduced by RSV. RSV down-regulated nuclear factor-κB activation, IκBα phosphorylation as well as activation of receptor-interacting protein2 and caspase-1 in HMC-1 cells. In addition, RSV treatment decreased the up-regulation of intracellular calcium in HMC-1 cells. Conclusions: These results suggest that RSV might be useful for the treatment of atopic diseases through blocking of TSLP.
Resin Infiltration in Dental Fluorosis Treatment—1-Year Follow-Up
Authors: Zotti Francesca , Albertini Luca , Tomizioli Nicolò , Capocasale Giorgia , Albanese Massimo
Published: December 2020

Background and Objective: Dental fluorosis is a disease affecting dental hard tissues featured with white or yellowish lesions. Several treatments are proposed in the literature, some even invasive. This clinical study aimed to evaluate the effectiveness of resin infiltration in terms of lesions resolution, trend of sensitive teeth and satisfaction of patients over time. Methods and Material: 200 fluorosis lesions were treated using ICON infiltrating resin (DMG, Hamburg, Germany). Parameters related to patients were collected by a questionnaire and analyzed aesthetic dissatisfaction about lesions, Shiff Air Index Sensitive Scale, sensitive teeth after treatment, the satisfaction of duration of treatment. The same operator measured dimensions of lesions Tooth Surface Index of Fluorosis (TSIF) and numbers of etching cycles needed for treating lesions. Statistical analysis was performed. The follow-up was of 1-year a measurement were performed at baseline (t0), immediately after the treatment (t1) and every three months during the observation period. Results: All lesions disappeared after one treatment. Pain or sensitive teeth were reported inside the 72 h and they disappeared after. Statistical analysis showed highly statistically correlation between etching cycles and the dimension of lesions and TSIF at the time-points evaluated as well as for pain during treatment, whereas a statistical significance was not noticed where etching cycles were correlated to sensitive teeth after 72 h. Overall, the treatment was found to be statistically significantly associated with differences in answers of aesthetic dissatisfaction between t0 and t1 and those collected between t1 and t2. Between t2 and t3 and between t3 and t4 no statistical differences were found in answers of patients about dissatisfaction, indicating the stability of the results. Conclusions: The ICON resin infiltration technique was found to be effective in lesions resolution with steady results.
A Systematic Review to Identify the Effects of Biologics in the Feet of Patients with Rheumatoid Arthritis
Authors: Ramos-Petersen Laura , Nester Christopher James , Reinoso-Cobo Andres , Nieto-Gil Pilar , Ortega-Avila Ana Belen , Gijon-Nogueron Gabriel
Published: December 2020

Background and Objective: Ninety percent of patients with rheumatoid arthritis (RA) feel foot pain during the disease process. Pharmacological treatment of RA has a systematic effect on the body and includes: Nonsteroidal anti-inflammatory drugs, disease-modifying antirheumatic drugs (DMARDs) and biologics. The objective of our review was to examine the impact of biologics on patients with RA ‘foot. Methods and Material: A systematic review of randomized control trials and observational studies that evaluated the efficacy of biologics against other pharmacological treatment, and included a foot outcome measure. The search covered MEDLINE Ovid, Pubmed, CINAHL, Cochrane Library, Evidence Search, and Web of Science. Risk of bias was evaluated using Cochrane guidance and the Newcastle Ottawa Scale adapted version. Results: A total of eight studies fully met the inclusion criteria: Three randomized control trials, and five observational studies were the basis of our review. A total sample of 1856 RA patients with RA treatment participated. The use of biologics was not associated as a risk factor for post-operative surgical site infection or delayed wound healing. The benefits of biologics, in terms of the disease evolution, were assessed using X-ray. Conclusion: Evidence suggests that the use of biologics is not a risk factor for post-operative surgical site infection or delayed wound healing. The use of biologics presents benefits in terms of the disease evolution assessed through X-ray.
Epigenetic-Based Biomarkers in the Malignant Transformation of BEAS-2B Cells Induced by Coal Tar Pitch Extract
Authors: Duan Shuyin , Yuan Huijie , Yu Songcheng , Wei Xiaoling , Zhou Xiaoshan , Wang Wei , Feng Feifei , Qu Lingbo , Wu Yongjun
Published: December 2020

Background and Objectives: The carcinogenicity of coal tar pitch (CTP) to occupational workers has been confirmed by the International Agency for Research on Cancer, especially for lung cancer. Herein, we explored the dynamic changes of epigenetic modifications in the malignant transformation process of CTP-induced BEAS-2B cells and also provided clues for screening early biomarkers of CTP-associated occupational lung cancer. Material and Methods: BEAS-2B cells treated with 3.0 μg/mL CTP extract (CTPE) were cultured to the 30th passage to set up a malignant transformation model, which was confirmed by platelet clone formation assay and xenograft assay. DNA methylation levels were determined by ultraviolet-high performance liquid chromatography. mRNA levels in cells and protein levels in supernatants were respectively detected by Real-Time PCR and enzyme-linked immunosorbent assay. Results: The number of clones and the ability of tumor formation in nude mice of CTPE-exposed BEAS-2B cells at 30th passage were significantly increased compared to vehicle control. Moreover, genomic DNA methylation level was down-regulated. The mRNA levels of DNMT1, DNMT3a and HDAC1 as well as the expression of DNMT1 protein were up-regulated since the 10th passage. From the 20th passage, the transcriptional levels of DNMT3b, let-7a and the expression of DNMT3a, DNMT3b, and HDAC1 proteins were detected to be higher than vehicle control, while the level of miR-21 increased only at the 30th passage. Conclusion: Data in this study indicated that the changes of epigenetic molecules including DNMT1, DNMT3a, DNMT3b, HDAC1, and let-7a occurred at the early stages of BEAS-2B cell malignant transformation after CTPE exposure, which provided critical information for screening early biomarkers of CTP-associated occupational lung cancer.
Composite Ferroelectric Membranes Based on Vinylidene Fluoride-Tetrafluoroethylene Copolymer and Polyvinylpyrrolidone for Wound Healing
Authors: Tverdokhlebova Tamara S. , Antipina Ludmila S. , Kudryavtseva Valeriya L. , Stankevich Ksenia S. , Kolesnik Ilya M. , Senokosova Evgenia A. , Velikanova Elena A. , Antonova Larisa V. , Vasilchenko Dmitry V. , Dambaev Georgiy T. , Plotnikov Evgenii V. , Bouznik Vyacheslav M. , Bolbasov Evgeny N.
Published: December 2020

Wound healing is a complex process and an ongoing challenge for modern medicine. Herein, we present the results of study of structure and properties of ferroelectric composite polymer membranes for wound healing. Membranes were fabricated by electrospinning from a solution of vinylidene fluoride/tetrafluoroethylene copolymer (VDF–TeFE) and polyvinylpyrrolidone (PVP) in dimethylformamide (DMF). The effects of the PVP content on the viscosity and conductivity of the spinning solution, DMF concentration, chemical composition, crystal structure, and conformation of VDF–TeFE macromolecules in the fabricated materials were studied. It was found that as PVP amount increased, the viscosity and conductivity of the spinning solutions decreased, resulting in thinner fibers. Using FTIR and XRD methods, it was shown that if the PVP content was lower than 50 wt %, the VDF–TeFE copolymer adopted a flat zigzag conformation (TTT conformation) and crystalline phases with ferroelectric properties were formed. Gas chromatography results indicated that an increase in the PVP concentration led to a higher residual amount of DMF in the material, causing cytotoxic effects on 3T3L1 fibroblasts. In vivo studies demonstrated that compared to classical gauze dressings impregnated with a solution of an antibacterial agent, ferroelectric composite membranes with 15 wt % PVP provided better conditions for the healing of purulent wounds.
Membrane Fractionation of Protein Hydrolysates from By-Products: Recovery of Valuable Compounds from Spent Yeasts
Authors: Vollet Marson Gabriela , Belleville Marie-Pierre , Lacour Stella , Dupas Hubinger Miriam
Published: December 2020

Spent brewer’s yeast (Saccharomyces sp.), the second most generated by-product from the brewing industry, contains bioactive and nutritional compounds with high added value such as proteins (40–50%), polysaccharides, fibers and vitamins. Molecules of interest from agro-industrial by-products need to be extracted, separated, concentrated, and/or purified so that a minimum purity level is achieved, allowing its application. Enzymatic hydrolysis has been successfully used in the production of peptides and protein hydrolysates. The obtained hydrolysates require efficient downstream processes such as membrane technology, which is an important tool for the recovery of thermolabile and sensitive compounds from complex mixtures, with low energy consumption and high specificity. The integration of membrane techniques that promote the separation through sieving and charge-based mechanisms is of great interest to improve the purity of the recovered fractions. This review is specifically addressed to the application of membrane technologies for the recovery of peptides from yeast protein hydrolysates. Fundamental concepts and practical aspects relative to the ultrafiltration of agro-industrial protein hydrolysates will be described. Challenges and perspectives involving the recovery of peptides from yeast protein hydrolysates will be presented and thoroughly discussed.
Metabolic Dynamics of In Vitro CD8+ T Cell Activation
Authors: Edwards-Hicks Joy , Mitterer Michael , Pearce Erika L. , Buescher Joerg M.
Published: December 2020

CD8+ T cells detect and kill infected or cancerous cells. When activated from their naïve state, T cells undergo a complex transition, including major metabolic reprogramming. Detailed resolution of metabolic dynamics is needed to advance the field of immunometabolism. Here, we outline methodologies that when utilized in parallel achieve broad coverage of the metabolome. Specifically, we used a combination of 2 flow injection analysis (FIA) and 3 liquid chromatography (LC) methods in combination with positive and negative mode high-resolution mass spectrometry (MS) to study the transition from naïve to effector T cells with fine-grained time resolution. Depending on the method, between 54% and 98% of measured metabolic features change in a time-dependent manner, with the major changes in both polar metabolites and lipids occurring in the first 48 h. The statistical analysis highlighted the remodeling of the polyamine biosynthesis pathway, with marked differences in the dynamics of precursors, intermediates, and cofactors. Moreover, phosphatidylcholines, the major class of membrane lipids, underwent a drastic shift in acyl chain composition with polyunsaturated species decreasing from 60% to 25% of the total pool and specifically depleting species containing a 20:4 fatty acid. We hope that this data set with a total of over 11,000 features recorded with multiple MS methodologies for 9 time points will be a useful resource for future work.
Cytotoxic Activity and Metabolic Profiling of Fifteen Euphorbia Species
Authors: El-Hawary Seham S. , Mohammed Rabab , Tawfike Ahmed F. , Lithy Nadia M. , AbouZid Sameh Fekry , Amin Mohamed N. , Abdelmohsen Usama Ramadan , Amin Elham
Published: December 2020

Euphorbia is a large genus of flowering plants with a great diversity in metabolic pattern. Testing the cytotoxic potential of fifteen Euphorbia species revealed highest activity of E. officinarum L. against human colon adenocarcinoma (CACO2) cell line (IC50 7.2 µM) and of E. lactea Haw. against human hepatoma (HepG2) and human breast adenocarcinoma (MCF-7) cell lines (IC50 5.2 and 5.1 µM, respectively). Additionally, metabolic profiling of the fifteen tested species, using LC-HRMS, for dereplication purposes, led to the annotation of 44 natural compounds. Among the annotated compounds, diterpenoids represent the major class. Dereplication approach and multivariate data analysis are adopted in order to annotate the compounds responsible for the detected cytotoxic activity. Results of Principle component analysis (PCA) come in a great accordance with results of biological testing, which emphasized the cytotoxic properties of E. lactea Haw. A similarity correlation network showed that the two compounds with the molecular formula C16H18O8 and C20H30O10, are responsible for cytotoxic activity against MCF-7 and HepG2 cell lines. Similarly, the compound with molecular formula C18H35NO correlates with cytotoxic activity against CACO2.
Effect of Ceramic Capillary Parameters on Bonded Morphology and Strength
Authors: Cao Jun , Zhang Junchao , Song Kexing , Wu Baoan , Ding Yong , Chen Dingbiao , Ding Yutian
Published: December 2020

The effects of the geometry parameters of a ceramic cleaver on the morphology of ball and second bonded points were studied using an automatic wire bonder, push pull tester, scanning electron microscope, ceramic capillary with different geometric parameters and φ25.4 μmAg-5Au bonding alloy wire, etc. The result shows that when the inner hole diameter (IHD) of the ceramic capillary is 1.3 times the diameter of the alloy wire (33 μm), the neck morphology of the ball bonded point (first bonded point) meet the requirements. The neck of the ball bonded point appeared to fracture when the IHD is 26 μm; The neck of the ball bonded point appeared as an irregular shape when the IHD is 41 μm. When the inner cutting angle diameter (ICAD) is 64 μm, the size of the mashed ball diameter (MBD) is qualified. When the ICAD is 51 μm, the MBD is too large and mashed ball overflows the pad. When the ICAD is 76 μm, the ball bonded point is too high. When the inner cutting bevel angle (ICBA) is 100°, the MBD size meets the requirements of the pad. When the ICBA was reduced to 70°, the ball bonded point is eccentric. When the ICBA was increased to 120°, the MBD is too large and is connected to the adjacent pad contact. The size of the fish tail of the second bonded point (second bonded point) changed in the same direction as the tip diameter (TD) changes. When the TD is 178 μm, the fish tail shape is regular and symmetrical. When the working face angle (WFA) is 8° and the outer circular radius (OCR) is equal to the diameter of the alloy wire (25.4 μm), the fish tail shape is regular. When the WFA is higher than 11° or the OCR is higher than 30 μm, the fish tail will appear as virtual welding, and when the WFA is less than 4°, the fish tail of the second bonded point will break due to thinning. When the OCR is less than 20 μm, the fish tail of the second bonded point is too long and causes a short circuit.