bluuebunny/crossref_metadata_2025 huggingface dataset:
Correlation between safety attitudes and early adoption of cognitive aids in the German culture sphere: a multicenter survey study
Authors: Wegener Justus , St.Pierre Michael , Keil Oliver , Eismann Hendrik
Published: September 2022

Background

Cognitive Aids (checklists) are a common tool to improve patient safety. But the factors for their successful implementation and continuous use are not yet fully understood. Recent publications suggest safety culture to play a key role in this context. However, the effects on the outcome of implementation measures remain unclear. Hospitals and clinics that are involved in cognitive aid development and research might have significantly different safety cultures than their counterparts, resulting in skewed assessments of proper implementation. Therefore, the objective of this study was to assess the correlation between cognitive aid implementation and safety attitudes of staff members in early adopting and later adopting clinics.

Methods

An online survey of the Safety Attitudes Questionnaire (SAQ) was carried out in German anaesthesiology departments during the initial implementation of a new checklist for emergencies during anesthesia (“eGENA” app). Subsequently an analysis between subgroups (“eGENA” app usage and occupation), with Kruskal–Wallis- and Mann–Whitney-U-Tests was carried out for the general SAQ, as well as it six subscales.

Results

Departments that introduced “eGENA” app (Median 3,74, IQR 0,90) reported a significantly higher median SAQ (U (NeGENA = 6, Nnon eGENA = 14) = 70,0, z = 2,31, p = 0,02, r = 0,516) than their counterparts (Median 2,82, IQR 0,77) with significant differences in the dimensions teamwork climate, work satisfaction, perception of management and working conditions.

Conclusion

Early adopters of cognitive aids are likely to show a significantly higher perception of safety culture in the SAQ. Consequently, successful implementation steps from these settings might not be sufficient in different clinics. Therefore, further investigation of the effects of safety culture on cognitive aid implementation should be conducted.
Scientific rewards for biomedical specialization are large and persistent
Authors: de Rassenfosse Gaétan , Higham Kyle , Penner Orion
Published: September 2022

Background

While specialization plays an essential role in how scientific research is pursued, we understand little about its effects on a researcher’s impact and career. In particular, the extent to which one specializes within their chosen fields likely has complex relationships with productivity, career stage, and eventual impact. Here, we develop a novel and fine-grained approach for measuring a researcher’s level of specialization at each point in their career and apply it to the publication data of almost 30,000 established biomedical researchers to measure the effect that specialization has on the impact of a researcher’s publications.

Results

Using a within-researcher, panel-based econometric framework, we arrive at several important results. First, there are significant scientific rewards for specialization—25% more citations per standard deviation increase in specialization. Second, these benefits are much higher early in a researcher’s career—as large as 75% per standard deviation increase in specialization. Third, rewards are higher for researchers who publish few papers relative to their peers. Finally, we find that, all else equal, researchers who make large changes in their research direction see generally increased impact.

Conclusions

The extent to which one specializes, particularly at the early stages of a biomedical research career, appears to play a significant role in determining the citation-based impact of their publications. When this measure of impact is, implicitly or explicitly, an input into decision-making processes within the scientific system (for example, for job opportunities, promotions, or invited talks), these findings lead to some important implications for the system-level organization of scientific research and the incentives that exist therein. We propose several mechanisms within modern scientific systems that likely lead to the scientific rewards we observe and discuss them within the broader context of reward structures in biomedicine and science more generally.
Rapid and sensitive single-cell RNA sequencing with SHERRY2
Authors: Di Lin , Liu Bo , Lyu Yuzhu , Zhao Shihui , Pang Yuhong , Zhang Chen , Wang Jianbin , Qi Hai , Shen Jie , Huang Yanyi
Published: September 2022

Background

Prevalent single-cell transcriptomic profiling (scRNA-seq) methods are mainly based on the synthesis and enrichment of full-length double-stranded complementary DNA. These approaches are challenging to generate accurate quantification of transcripts when their abundance is low or their full-length amplifications are difficult.

Results

Based on our previous finding that Tn5 transposase can directly cut-and-tag DNA/RNA hetero-duplexes, we present SHERRY2, a specifically optimized protocol for scRNA-seq without second-strand cDNA synthesis. SHERRY2 is free of pre-amplification and eliminates the sequence-dependent bias. In comparison with other widely used scRNA-seq methods, SHERRY2 exhibits significantly higher sensitivity and accuracy even for single nuclei. Besides, SHERRY2 is simple and robust and can be easily scaled up to high-throughput experiments. When testing single lymphocytes and neuron nuclei, SHERRY2 not only obtained accurate countings of transcription factors and long non-coding RNAs, but also provided bias-free results that enriched genes in specific cellular components or functions, which outperformed other protocols. With a few thousand cells sequenced by SHERRY2, we confirmed the expression and dynamics of Myc in different cell types of germinal centers, which were previously only revealed by gene-specific amplification methods.

Conclusions

SHERRY2 is able to provide high sensitivity, high accuracy, and high throughput for those applications that require a high number of genes identified in each cell. It can reveal the subtle transcriptomic difference between cells and facilitate important biological discoveries.
Disease-associated regulation of gene expression by resveratrol: Special focus on the PI3K/AKT signaling pathway
Authors: Ghafouri-Fard Soudeh , Bahroudi Zahra , Shoorei Hamed , Hussen Bashdar Mahmud , Talebi Seyedeh Fahimeh , Baig Sadia Ghousia , Taheri Mohammad , Ayatollahi Seyed Abdulmajid
Published: September 2022

Resveratrol (3,5,4′-trihydroxy-trans-stilbene) is a natural phenol that is present in the skin of the grape, blueberry, raspberry, mulberry, and peanut. This substance is synthesized in these plants following injury or exposure to pathogens. Resveratrol is used as a dietary supplement for a long time and its effects have been assessed in animal models of human disorders. It has potential beneficial effects in diverse pathological conditions such as diabetes mellitus, obesity, hypertension, neoplastic conditions, Alzheimer's disease, and cardiovascular disorders. Notably, resveratrol has been found to affect the expression of several genes including cytokine coding genes, caspases, matrix metalloproteinases, adhesion molecules, and growth factors. Moreover, it can modulate the activity of several signaling pathways such as PI3K/AKT, Wnt, NF-κB, and Notch pathways. In the current review, we summarize the results of studies that reported modulatory effects of resveratrol on the expression of genes and the activity of signaling pathways. We explain these results in two distinct sections of non-neoplastic and neoplastic conditions.
Law enforcement and syringe services program implementation in rural counties in Kentucky: a qualitative exploration
Authors: Allen Sean T. , Danforth Sarah , Grieb Suzanne M. , Glick Jennifer L. , Harris Samantha J. , Tomko Catherine , Sherman Susan G.
Published: September 2022

Background

Existing research in urban areas has documented a multitude of ways in which law enforcement may affect risks for bloodborne infectious disease acquisition among people who inject drugs (PWID), such as via syringe confiscation and engaging in practices that deter persons from accessing syringe services programs (SSPs). However, limited work has been conducted to explore how law enforcement may impact SSP implementation and operations in rural counties in the United States. This creates a significant gap in the HIV prevention literature given the volume of non-urban counties in the United States that are vulnerable to injection drug use-associated morbidity and mortality.

Objective

This study explores the influence of law enforcement during processes to acquire approvals for SSP implementation and subsequent program operations in rural Kentucky counties.

Methods

From August 2020 to October 2020, we conducted eighteen in-depth qualitative interviews among persons involved with SSP implementation in rural counties in Kentucky (USA). Interviews explored the factors that served as barriers and facilitators to SSP implementation and operations, including the role of law enforcement.

Results

Participants described scenarios in which rural law enforcement advocated for SSP implementation; however, they also reported police opposing rural SSP implementation and engaging in adverse behaviors (e.g., targeting SSP clients) that may jeopardize the public health of PWID. Participants reported that efforts to educate rural law enforcement about SSPs were particularly impactful when they discussed how SSP implementation may prevent needlestick injuries.

Conclusions

The results of this study suggest that there are multiple ways in which rural SSP implementation and subsequent operations in rural Kentucky counties are affected by law enforcement. Future work is needed to explore how to expeditiously engage rural law enforcement, and communities more broadly, about SSPs, their benefits, and public health necessity.
Knockdown circTRIM28 enhances tamoxifen sensitivity via the miR-409-3p/HMGA2 axis in breast cancer
Authors: Yang Shiyong , Zou Changwu , Li Yuxin , Yang Xianguo , Liu Wei , Zhang Guannan , Lu Nina
Published: September 2022

Background

Tamoxifen (TAM) is a frequently-used treatment for breast cancer (BC). But the TAM resistance seriously affects the patient therapeutic effect. Previous research indicated that circular RNAs (circRNAs) might participate in the regulatory processes of BC. Here, we discovered the parts of circular RNA tripartite motif-containing 28 (circTRIM28) in BC.

Methods

CircTRIM28, microRNA-409-3p (miR-409-3p), and high mobility group AT-hook 2 (HMGA2) levels were perceived by qRT-PCR and western blot. Moreover, the biological functions of the cells were examined. Furthermore, dual-luciferase report was employed to reconnoiter the targeted relationship between miR-409-3p and circTRIM28 or HMGA2.

Results

CircTRIM28 and HMGA2 were augmented, and the miR-409-3p was repressed in BC. Silencing circTRIM28 enhanced tamoxifen sensitivity and cell apoptosis, whereas hampered cell development in BC cells. In mechanism, circTRIM28 could sponge miR-409-3p to increase HMGA2. In addition, silencing circTRIM28 impeded tumor growth.

Conclusion

CircTRIM28 facilitated the BC via miR-409-3p/HMGA2.
Identification of SSBP1 as a ferroptosis-related biomarker of glioblastoma based on a novel mitochondria-related gene risk model and in vitro experiments
Authors: Su Jun , Li Yue , Liu Qing , Peng Gang , Qin Chaoying , Li Yang
Published: September 2022

Background

Glioblastoma (GBM) is the most common primary malignant brain tumor that leads to lethality. Several studies have demonstrated that mitochondria play an important role in GBM and that mitochondria-related genes (MRGs) are potential therapeutic targets. However, the role of MRGs in GBM remains unclear.

Methods

Differential expression and univariate Cox regression analyses were combined to screen for prognostic differentially-expressed (DE)-MRGs in GBM. Based on LASSO Cox analysis, 12 DE-MRGs were selected to construct a risk score model. Survival, time dependent ROC, and stratified analyses were performed to evaluate the performance of this risk model. Mutation and functional enrichment analyses were performed to determine the potential mechanism of the risk score. Immune cell infiltration analysis was used to determine the association between the risk score and immune cell infiltration levels. CCK-8 and transwell assays were performed to evaluate cell proliferation and migration, respectively. Mitochondrial reactive oxygen species (ROS) levels and morphology were measured using a confocal laser scanning microscope. Genes and proteins expression levels were investigated by quantitative PCR and western blotting, respectively.

Results

We identified 21 prognostic DE-MRGs, of which 12 DE-MRGs were selected to construct a prognostic risk score model for GBM. This model presented excellent performance in predicting the prognosis of patients with GBM and acted as an independent predictive factor. Functional enrichment analysis revealed that the risk score was enriched in the inflammatory response, extracellular matrix, and pro-cancer-related and immune related pathways. Additionally, the risk score was significantly associated with gene mutations and immune cell infiltration in GBM. Single-stranded DNA-binding protein 1 (SSBP1) was considerably upregulated in GBM and associated with poor prognosis. Furthermore, SSBP1 knockdown inhibited GBM cell progression and migration. Mechanistically, SSBP1 knockdown resulted in mitochondrial dysfunction and increased ROS levels, which, in turn, increased temozolomide (TMZ) sensitivity in GBM cells by enhancing ferroptosis.

Conclusion

Our 12 DE-MRGs-based prognostic model can predict the GBM patients prognosis and 12 MRGs are potential targets for the treatment of GBM. SSBP1 was significantly upregulated in GBM and protected U87 cells from TMZ-induced ferroptosis, which could serve as a prognostic and therapeutic target/biomarker for GBM.
Analysis of risk factors of multiorgan failure after pericardiectomy for constrictive pericarditis
Authors: Huang Jing-bin , Wen Zhao-ke , Yang Jian-rong , Li Jun-jun , Li Min , Lu Chang-chao , Liang Da-ying , Wei Cheng-xin
Published: September 2022

Background

We aimed to investigate risk factors of multiorgan failure following pericardiectomy.

Methods

This was a retrospective study of patients undergoing pericardiectomy between January 1994 and May 2021 at three hospitals.

Results

826 patients were included in the study and divided into two groups: group with multiorgan failure (n = 86) and group without multiorgan failure (n = 740). There were 86 patients with multiorgan failure (86/826, 10.4%). There were 66 operative deaths (66/826, 8.0%). The causes of operative deaths were multiorgan failure, including cardiogenic shock + AKI + ventricular fibrillation (13/66), cardiogenic shock + AKI (35/66), cardiogenic shock + AKI + hepatic failure + septicemia (8/66), cardiogenic shock + AKI + respiratory failure (10/66). Univariate and multivariate analyses showed the factors associated with multiorgan failure, including male (P = 0.006), time between symptoms and surgery (P < 0.001), thickness of pericardium (P < 0.001), intubation time (P < 0.001), ICU retention time (P < 0.001), hospitalized time postoperative (P < 0.001), preoperative central venous pressure (P < 0.001), postoperative central venous pressure (P < 0.001), D0 fluid balance (P < 0.001), D2 fluid balance (P < 0.001), postoperative chest drainage (P < 0.001), preoperative LVEDD(P < 0.001), postoperative LVEDD (P < 0.001), surgical duration (P < 0.001), bleeding during operation (P < 0.001), serum creatinine 24 h after surgery (P = 0.042), serum creatinine 48 h after surgery (P < 0.001), fresh-frozen plasma (P < 0.001), packed red cells (P < 0.001), blood lactate (P < 0.001).

Conclusion

In our study, incomplete pericardial dissection, fluid overload, delayed diagnosis and treatment are associated with multiorgan failure following pericardiectomy.
The COVID-19 pandemic as a catalyst for innovation: a regulatory framework to assess fit-for-purpose innovative approaches in clinical research
Authors: Leyens Lada , Simkins Tracy , Horst Nafsika Kronidou
Published: September 2022

The COVID-19 pandemic has had a devastating impact on individuals and multiple aspects of our society including healthcare and clinical research. The silver lining is that the pandemic also served as a catalyst for wider adoption of innovative approaches in clinical research, notably the use of mobile or remote services, and digital technologies. Regulators, clinical study investigators, clinical study participants, sponsors, and other stakeholders collaborated to adopt measures that ensured safe participation in clinical studies whilst maintaining study integrity. In this article, we propose a regulatory framework for assessing fit-for-purpose innovative approaches in clinical research based on Roche/Genentech’s experience during the COVID-19 pandemic with the aim to inform and encourage broader implementation of patient-centric and sustainable innovation in clinical research. Our goal is to contribute to ongoing discussions on introducing innovative approaches in clinical trials and eventually the development of globally harmonised guidelines.
Assigning Culicoides larvae to species using DNA barcoding of adult females and phylogenetic associations
Authors: Jin Tao , Husseneder Claudia , Foil Lane
Published: September 2022

Background

Orbivirus-induced hemorrhagic diseases cause high mortality in wild and captive white-tailed deer in North America. The role of differentCulicoidesspecies inOrbivirustransmission outside of areas of intensive animal production has not been established. At our study location, bluetongue virus (BTV) RNA-positive femaleCulicoides debilipalpispools have been detected annually since 2012 when BTV transmission was noted in a captive deer herd. Identifying specific larval habitats of suspected vectors at active transmission sites is crucial both for identifying the source of the vectors and for subsequently planning intervention actions. SinceC. debilipalpislarvae are known to develop in tree holes, this study was designed to use DNA barcoding to identify larvae collected from tree holes.

Methods

Adult femaleCulicoideswere collected using light or emergence traps and morphologically identified to 11 species.Culicoides sonorensiswere also obtained from a laboratory colony. Substrate was collected from tree holes and flooded with water to harvest floating larvae. Total DNA from three to seven adult females per species and 19 larvae was extracted. Two loci of the nuclear 18S ribosomal RNA (rRNA) gene, one locus each of the mitochondrial cytochrome oxidase subunit I (COI) gene and the nuclear 28S rRNA gene were amplified using loci-specific primers.

Results

All 61 adults were sequenced at each of the four loci under study. Since no single locus delineated all putative species and the COI locus yielded unreliable pseudogenes for two individuals ofC. arboricola, sequences of all four loci were concatenated to maximize species separation and allow for larval association with identified adults. Sixteen larvae were clearly assigned to species based on DNA barcoding and phylogenetic results. Multiple larvae were assigned to each of theC. debilipalpisclade, theC. villosipennisclade, theC. arboricolaclade and theC. nanusclade.

Conclusions

Of the approximately 62 species described in the southeast USA, 21 have now been barcoded and sequences are publicly available. In this study, we constructed a database composed of species-specific sequences of adultCulicoidesand then identified larvae to species by matching their corresponding sequences with adults. SinceCulicoideslarvae are difficult to identify, using DNA barcoding to facilitate larval habitat surveys can be a valuable tool.

Graphical Abstract
Circ_0000554 is identified as a cancer-promoting circRNA in colorectal cancer by regulating the miR-1205/LASP1 axis
Authors: Luo Jinlong , Yang Hua , Peng Xuefeng , Zhang Faqiang , Shu Shilong , Lan Ke , Tu Shengjin , Lu Kai , Cha Xiaoying
Published: September 2022

Background

Colorectal cancer (CRC) is a prevalent malignant tumor with poor prognosis. Circular RNAs (circRNAs) are key regulators in the progression of CRC. Our study aimed to disclose the role of circ_0000554 in CRC.

Methods

The expression of circ_0000554, miR-1205 and LIM and SH3 protein 1 (LASP1) was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation, invasion and migration were monitored using cell counting kit-8 (CCK-8) assay, EdU assay, transwell assay and wound healing assay respectively. The protein levels of C-myc, matrix metallopeptidase 2 (MMP-2) and LASP1 were detected by western blot. Tumor formation assay in nude mice was conducted to explore the role of circ_0000554 in vivo. The association between miR-1205 and circ_0000554 or LASP1 was identified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay.

Results

circ_0000554 was upregulated in CRC tissues and cells, high circ_0000554 expression was significantly linked to shorter overall survival. Downregulation of circ_0000554 restrained cell growth and metastasis while promoted apoptosis in vitro, and suppressed tumorigenesis of CRC in vivo. Furthermore, mechanism study and rescue experiments confirmed miR-1205 could be sponged by circ_0000554 and its inhibitor reversed the inhibitory effect of circ_0000554 silencing on CRC progression. LASP1 was a target gene of miR-1205 and the upregulation of LASP1 overturned miR-1205-induced effects on CRC cells. Circ_0000554 could elevate LASP1 expression via interacting with miR-1205.
Spatial behavior of red deer (Cervus elaphus) in Northern Apennines: are we managing them correctly?
Authors: Fontana Riccardo , Calabrese Licia , Lanzi Ambrogio , Armaroli Elisa , Raganella Pelliccioni Elisabetta
Published: September 2022

Background

Throughout their range, red deer are a well-studied species. In Italy, this species occupies two ecologically different ranges: the Alps and the Apennines. Although several studies have described the spatial behaviour of red deer in the Italian Alps, no data are available for the Apennine population.

Results

The spatial behaviours of 13 deer from the Northern Apennines range were analysed for the first time using GPS-GSM telemetry from 2011 to 2017. Red deer displayed two coexisting strategies, i.e., migratory and stationary. In our sample, females tended to migrate more than males. We found a high level of interindividual variability in the date of migration/return, while each migratory deer was very conservative during the study period. The migration ranges were on average 12 ± 4.2 km from the resident range. Both migratory and resident deer displayed high site fidelity. No switch from the migratory to stationary strategy was observed for any deer during the study period; however, the period could have been too short to detect any switch. At the management level, over 18 management cycles occurred during the study period, and a spatial mismatch was found between deer range and management units (districts) in 38.9% (7) of the cases. Merging the districts belonging to each province to obtain an area of approximately 1000 km2would partially address such spatial mismatch, reducing its occurrence to 22%.

Conclusions

Despite the small sample size, these results can guide future management actions. However, an in-depth study with a larger sample size is required to better understand and manage the red deer Apennines population.
Ex vivo expanded human regulatory T cells modify neuroinflammation in a preclinical model of Alzheimer’s disease
Authors: Faridar Alireza , Vasquez Matthew , Thome Aaron D. , Yin Zheng , Xuan Hui , Wang Jing Hong , Wen Shixiang , Li Xuping , Thonhoff Jason R. , Zhao Weihua , Zhao Hong , Beers David R. , Wong Stephen T. C. , Masdeu Joseph C. , Appel Stanley H.
Published: September 2022

Background

Regulatory T cells (Tregs) play a neuroprotective role by suppressing microglia and macrophage-mediated inflammation and modulating adaptive immune reactions. We previously documented that Treg immunomodulatory mechanisms are compromised in Alzheimer’s disease (AD). Ex vivo expansion of Tregs restores and amplifies their immunosuppressive functions in vitro. A key question is whether adoptive transfer of ex vivo expanded human Tregs can suppress neuroinflammation and amyloid pathology in a preclinical mouse model.

Methods

An immunodeficient mouse model of AD was generated by backcrossing the 5xFAD onto Rag2 knockout mice (5xFAD-Rag2KO). Human Tregs were expanded ex vivo for 24 days and administered to 5xFAD-Rag2KO. Changes in amyloid burden, microglia characteristics and reactive astrocytes were evaluated using ELISA and confocal microscopy. NanoString Mouse AD multiplex gene expression analysis was applied to explore the impact of ex vivo expanded Tregs on the neuroinflammation transcriptome.

Results

Elimination of mature B and T lymphocytes and natural killer cells in 5xFAD-Rag2KO mice was associated with upregulation of 95 inflammation genes and amplified number of reactive microglia within the dentate gyrus. Administration of ex vivo expanded Tregs reduced amyloid burden and reactive glial cells in the dentate gyrus and frontal cortex of 5xFAD-Rag2KO mice. Interrogation of inflammation gene expression documented down-regulation of pro-inflammatory cytokines (IL1A&B, IL6), complement cascade (C1qa, C1qb, C1qc, C4a/b), toll-like receptors (Tlr3, Tlr4 and Tlr7) and microglial activations markers (CD14, Tyrobp,Trem2) following Treg administration.

Conclusions

Ex vivo expanded Tregs with amplified immunomodulatory function, suppressed neuroinflammation and alleviated AD pathology in vivo. Our results provide preclinical evidences for Treg cell therapy as a potential treatment strategy in AD.
Grain boundary passivation via balancing feedback of hole barrier modulation in HfO2-x for nanoscale flexible electronics
Authors: Kim Yeon Soo , Chung Harry , Kwon Suhyoun , Kim Jihyun , Jo William
Published: September 2022

Flexible electronics has attracted considerable attention owing to its enormous potential for practical applications in various fields. However, the massive strain produced during bending degrades the device. Especially at grain boundaries, due to the accumulation of defects, this degradation is exacerbated in flexible electronic devices. The importance of electrically inactivated grain boundaries increases as devices scale down to the nanoscale. Here, we propose an HfO2-xthin film that can be used as an excellent material for flexible electronics with versatile functionality, especially for grain boundary passivation. Various electrical phases of HfO2-xthin films with conducting to insulating behavior, which originates from oxygen deficiency, have been fabricated on flexible substrates. Furthermore, owing to the most stable charge state of oxygen vacancies, oxygen-deficient HfO2-xshowsp-type conductivity. Current mapping by conductive atomic force microscopy reveals that current flow is hindered at grain boundaries due to the formation of potential barriers. This phenomenon is also observed in bent flexible thin films on convex and concave molds, leading to tensile and compressive strains, respectively. Although the defect concentration increases because of lattice deformation during bending, more holes are trapped at the grain boundaries, resulting in an increased hole barrier height. We believe that grain boundary passivation through hole barrier modulation during bending would pave the way for advances in hafnia-based nanoscale flexible electronics.
Acute and Chronic Effects of Blood Flow Restricted High-Intensity Interval Training: A Systematic Review
Authors: Chua Man Tong , Sim Alexiaa , Burns Stephen Francis
Published: September 2022

Background

The implementation of blood flow restriction (BFR) during exercise is becoming an increasingly useful adjunct method in both athletic and rehabilitative settings. Advantages in pairing BFR with training can be observed in two scenarios: (1) training at lower absolute intensities (e.g. walking) elicits adaptations akin to high-intensity sessions (e.g. running intervals); (2) when performing exercise at moderate to high intensities, higher physiological stimulus may be attained, leading to larger improvements in aerobic, anaerobic, and muscular parameters. The former has been well documented in recent systematic reviews, but consensus on BFR (concomitant or post-exercise) combined with high-intensity interval training (HIIT) protocols is not well established. Therefore, this systematic review evaluates the acute and chronic effects of BFR + HIIT.

Methods

The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were used to identify relevant studies. A systematic search on 1 February 2022, was conducted on four key databases: ScienceDirect, PubMed, Scopus and SPORTDiscus. Quality of each individual study was assessed using the Physiotherapy Evidence Database (PEDro) scale. Extraction of data from included studies was conducted using an adapted version of the 'Population, Intervention, Comparison, Outcome' (PICO) framework.

Results

A total of 208 articles were identified, 18 of which met inclusion criteria. Of the 18 BFR + HIIT studies (244 subjects), 1 reported both acute and chronic effects, 5 examined acute responses and 12 investigated chronic effects. Acutely, BFR challenges the metabolic processes (vascular and oxygenation responses) during high-intensity repeated sprint exercise—which accelerates central and peripheral neuromuscular fatigue mechanisms resulting in performance impairments. Analysis of the literature exploring the chronic effects of BFR + HIIT suggests that BFR does provide an additive physiological training stimulus to HIIT protocols, especially for measured aerobic, muscular, and, to some extent, anaerobic parameters.

Conclusion

Presently, it appears that the addition of BFR into HIIT enhances physiological improvements in aerobic, muscular, and, to some extent, anaerobic performance. However due to large variability in permutations of BFR + HIIT methodologies, it is necessary for future research to explore and recommend standardised BFR guidelines for each HIIT exercise type.
Time–frequency co-movement and risk connectedness among cryptocurrencies: new evidence from the higher-order moments before and during the COVID-19 pandemic
Authors: Cui Jinxin , Maghyereh Aktham
Published: September 2022

Analyzing comovements and connectedness is critical for providing significant implications for crypto-portfolio risk management. However, most existing research focuses on the lower-order moment nexus (i.e. the return and volatility interactions). For the first time, this study investigates the higher-order moment comovements and risk connectedness among cryptocurrencies before and during the COVID-19 pandemic in both the time and frequency domains. We combine the realized moment measures and wavelet coherence, and the newly proposed time-varying parameter vector autoregression-based frequency connectedness approach (Chatziantoniou et al. in Integration and risk transmission in the market for crude oil a time-varying parameter frequency connectedness approach. Technical report, University of Pretoria, Department of Economics, 2021) using intraday high-frequency data. The empirical results demonstrate that the comovement of realized volatility between BTC and other cryptocurrencies is stronger than that of the realized skewness, realized kurtosis, and signed jump variation. The comovements among cryptocurrencies are both time-dependent and frequency-dependent. Besides the volatility spillovers, the risk spillovers of high-order moments and jumps are also significant, although their magnitudes vary with moments, making them moment-dependent as well and are lower than volatility connectedness. Frequency connectedness demonstrates that the risk connectedness is mainly transmitted in the short term (1–7 days). Furthermore, the total dynamic connectedness of all realized moments is time-varying and has been significantly affected by the outbreak of the COVID-19 pandemic. Several practical implications are drawn for crypto investors, portfolio managers, regulators, and policymakers in optimizing their investment and risk management tactics.
A case of severe generalized pustular psoriasis successfully treated with IL-17A monoclonal antibody and granulocyte removal therapy
Authors: Shimada Keiki , Katagiri Daisuke , Kato Aika , Nunose Naoto , Sato Motohiko , Katayama Yuri , Terakawa Kanako , Niikura Takahito , Sakamoto Emi , Yoshizaki Yuki , Suzuki Minami , Fukaya Takashi , Tamaki Takeshi , Takano Hideki
Published: September 2022

Background

Generalized pustular psoriasis (GPP) usually presents with fever, generalized flushing, and multiple sterile pustules on the skin, which histopathologically form subcorneal pustules characterized by Kogoj spongiform pustules. Granulocyte/monocyte adsorption apheresis (GMA) was approved in Japan in 2012. The use of biologics for psoriasis treatment is increasing. Several case reports have evaluated the combination of GMA and cyclosporine (CyA) for GPP. However, very few English reports on combining biologics and GMA in treating GPP exist.

Case presentation

A 79-year-old man with a history of hypertension, diabetes mellitus, chronic kidney disease, and atrial fibrillation was admitted. He had been consulting a dermatologist for psoriasis vulgaris (PV) since the age of 44. The patient was diagnosed with severe GPP and treated with 300 mg secukinumab (SEC) on day 3. SEC is a fully human monoclonal IgG1 antibody that targets IL-17A. Five doses were administered. In addition, GMA was administered once a week, three times from day 4. After the first administration of GMA, the inflammatory response and skin condition improved markedly. The patient was discharged from the hospital on day 34.

Conclusions

The present study is the first English-written report on the combined administration of SEC and GMA both instituted since admission for severe GPP, with immediate patient response to treatment. Notably, IL-17A plays a vital role in the pathogenesis of GPP. GMA can eliminate activated leukocytes, and the early introduction of combined IL-17 monoclonal antibody and GMA may allow disease suppression in patients with severe GPP, thus avoiding progression to multiorgan failure. Further studies may verify the effects of IL-17 monoclonal antibodies and GMA on severe GPP.
Effect of entomopathogenic fungi against banana pseudostem weevil Odoiporus longicollis (Olivier) and elucidation of infection process
Authors: Viswakethu Velavan , Ramasamy Vinitha , Krishnamoorthy Saranya , Segar Thilakshana , Ramaraj Aravindh , Kuttithodika Muhammed Aboobacker , Narayanasamy Baskar , Balakrishnan Padmanaban , Rajagopal Rangeshwaran
Published: September 2022

Background

The banana stem weevil,Odoiporus longicollis(Olivier), is a serious threat to banana cultivation world over. Since banana is a food crop, the use of naturally infecting biological control agents could be an effective alternative to manage the insect pest instead of harmful chemicals. Also, the efficacy of entomopathogenic fungi againstO. longicolliswas used in bioassay.

Results

Among theBeauveria bassianaisolates tested the median lethal concentration (LC50) 10.468 × 105conidia ml−1when treated withB. bassiana(NRCBEFPMP1), two other isolates ofB. bassiana, namely NRCBEPF22 and NRCBEPF2, were also effective againstO. longicollisand recorded LC50of 12.617 × 105and 12.891 × 105conidia ml−1, respectively. The results of bioassay with differentMetarhiziumspp. showed variations in efficacy, where the most virulent isolate wasM. quizhouense(NRCBEPF11) with LC508.050 × 105conidia ml−1. Scanning electron microscopic analysis showed thatB. bassianaandM. quizhouensecaused infection by cuticle penetration and completed the infection process in 15 days. The composition of volatile organic compounds released byB. bassianaandM. anisopliaeduring pathogenesis showed that a significantly high number of known insect volatiles were present in infected insects. Consequently, these volatiles were emission in Insect attractant, Odorant receptor agonist, Plant hormone Plant, and Microbial Metabolites, through the biological activity, such as Methyl salicylate, Benzaldehyde, alpha-Terpineol, Limonene, Benzene, 1,2-dimethoxy, Phthalic acid, 1-Octadecene, Phenylacetaldehyde, 3-Octanone, Octanal, Methylheptenone and 2-Ethyl-1-hexyl alcohol.

Conclusion

Overall, the results show that EPF could significantly reduce damage byO. longicollisand produce a wide profile of secondary metabolites. Further, analysis was used for principal components to determine whether separated classes of fungi can be distinguished from one another based on their metabolite profiles.
Influence of perioperative anesthesia methods or anesthetic agents preferred for gastric cancer surgery on the survival of patients: a narrative review
Authors: Yılmaz Fulya , Bas Koray
Published: September 2022

Background

Gastric cancer is a major global public health problem. It is the fourth most common cancer and the second cause of cancer-related deaths worldwide. Despite advances in the field of medical and radiation oncology, surgical resection is a crucial intervention and remains the mainstay of gold standard treatment. Recently, the effects of anesthesia method(s) and/or anesthetic agent(s) on survival for different types of cancers gained attention. So, we want to summarize the evidences of anesthesia methods and/or anesthetic agents preferred for gastric cancer surgery on the survival.

Main body

The Web of Science software was used for the search and the analysis. To analyze scientific productivity of all scientific papers published about survival of patients due to the anesthesia methods or anesthetic agents on gastric cancer in Science Citation Index Expanded (SCI-E) from 1980 to December 5, 2020, the date of the search was searched by using the terms of “gastric cancer,” “survival,” and “anesthesia” in the topic search section of the software.

As a result, overall, fifteen papers were related to our topic. Four of these studies compared total intravenous anesthesia (TIVA) with general anesthesia, five of these compared general anesthesia with general anesthesia combined with epidural anesthesia/analgesia for gastric cancer, and three of these studies investigated effect of anesthetic agents for gastric cells in in vitro conditions. Other publications were review on this topic.

Conclusions

The important role of anesthesia in treatment of gastric cancer patients is still controversial. Further prospective randomized studies are needed.
Effects of dietary Syzygium aromaticum leaf meal supplementation on blood profile and oxidative status of laying hens
Authors: Adu Olufemi Adesanya , Olateju Imoleayo Sarah , Samuel Adeniyi David , Gbore Francis Ayodeji
Published: September 2022

Background

The study examined the effects of dietary supplementation of Syzygium aromaticum leaf meal (SLM) on the haematology, serum biochemistry and antioxidative status of laying hens.

Results

A total of 150 20-week-old Isa brown birds were randomly assigned to three experimental diets: Diet 1/control (no supplementation), Diet 2 (0.25% SLM) and Diet 3 (0.50% SLM). Each experimental diet was further divided into five (5) replicates and comprised 10 birds per replicate, that is, 50 birds per experimental diet. The birds were fed with layers mash for 32 weeks of the feeding trial. Blood samples collected in the 32nd week were subjected to haematological and serum biochemical analyses. The dietary supplementation of 0.25% and 0.50% SLM did not significantly (P > 0.05) affect the haematological parameters observed in the layer birds which indicated that SLM did not render the birds anaemic nor compromise their immunity. Likewise, the result of the serum protein (total protein, albumin, globulin and albumin: globulin), serum enzymes (alanine and aspartate aminotransferases) and serum metabolites (creatinine, uric acid and glucose) were not affected (P > 0.05) by the dietary treatment except for the concentration of serum catalase and glutathione peroxidase which was statistically higher (P < 0.05) in layers fed with diets supplemented with SLM compared to the control group, while the serum superoxide dismutase concentration of laying hens was not significantly influenced (P > 0.05).

Conclusion

The supplementation of SLM at 0.25% and 0.50% in layers diet can be referred to as a safe additive as the blood indices of birds were not adversely affected. Also, the liver functions which were investigated through the serum enzymes were not impaired and oxidative stress is not triggered in the birds.
Exploring the feasibility of ex-post harmonisation of religiosity items from the European Social Survey and the European Values Study
Authors: Aizpurua Eva , Fitzgerald Rory , de Barros Julia Furtado , Giacomin Gregorio , Lomazzi Vera , Luijkx Ruud , Maineri Angelica , Negoita Daniela
Published: September 2022

This paper examines the feasibility of ex-post harmonisation strategies using European Values Study (EVS) Wave 5 (2017–2020) and European Social Survey (ESS) Round 9 (2018–2019) data across 17 countries. The study shows an empirical assessment of the comparability of four items measuring religious behaviours (belonging to a religious denomination at present/in the past, religious services attendance, and praying), captured in both surveys. The novelty of this paper lies in the analytical comparison of religiosity indicators that are rarely assessed from a comparative perspective.

The harmonisation strategy was based upon several analytical techniques that seek to determine similarities and differences between the selected items in terms of (a) their validity, by examining their correlations with a set of sociodemographic and substantive correlates, (b) their distributions, supplemented by visual comparisons and relevant statistical tests, and (c) item non-substantive shares. The findings pointed to the most consistency among the partial correlations, where individual religiosity produced the most differences between the surveys. Distributions produced the most discrepancies that also corresponded to less similarity across variable categories as gauged by Duncan’s index. This paper is descriptive and exploratory in its aim. It can be taken as a jumping-off point for future research where the time series of these two surveys, and potentially others, can be examined across aggregate levels (e.g. birth cohorts, countries).
Role of Strauss ECG criteria as predictor of response in patients undergoing cardiac resynchronization therapy
Authors: Shoman Khaled Ashraf , Eldamanhory Hayam Mohammed , Fakhry Emad Effat , Badran Haitham Abdelfatah
Published: September 2022

Background

Cardiac resynchronization therapy (CRT) is a standard treatment in patients with heart failure; however, approximately 20–40% of recipients of (CRT) do not respond to it based on the current patients’ selection criteria. The purpose of this study was to identify the baseline parameters that predict the CRT response and how the ECG morphology can affect the outcome. The study aimed to evaluate the Strauss ECG criteria as a predictor of response in patients undergoing cardiac resynchronization therapy.

Results

Out of 70 patients, 3 patients missed the 6-month follow-up after CRT implantation, so the study enrolled 67 patients that have been classified according to ECG morphology of LBBB to 37 patients with non-Strauss ECG criteria—one of whom died after 4 months—and 30 patients with Strauss ECG criteria. The number of responders in the study was 50 patients with percentage 75.8%; 52% of CRT responder (26 patients) had non-Strauss ECG criteria, while 48% of CRT responders (24 patients) had Strauss ECG criteria with P value = 0.463. While there was no statistical significance of overall CRT response nor 6-month hospitalization and mortality between patients of Strauss and non-Strauss ECG criteria, there was a significant improvement in NYHA class, EF assessed by biplane Simpson’s, end-systolic volume, global longitudinal strain and global circumferential strain by speckle tracking echocardiography in patients with Strauss ECG criteria of LBBB.

Conclusions

There is no statistical significance in overall CRT response nor the 6-month hospitalization and mortality after 6 months of follow-up between patients with Strauss and non-Strauss ECG criteria of LBBB; however, patients with Strauss ECG criteria have better improvement in NYHA class, echocardiographic parameters such as EF and ESV and speckle tracking parameters (GLS and GCS).
Anticancer cytotoxicity and antifungal abilities of green-synthesized cobalt hydroxide (Co(OH)2) nanoparticles using Lantana camara L.
Authors: Ravi Lokesh , Sreenivas B. K. Anirudh , Kumari G. R. Shree , Archana O.
Published: September 2022

Background

Green synthesis of metal nanoparticles with pharmaceutical applications is the current focus in the field of nanomedicine. This study aims at use of Lantanacamara L as a source of green reducing agent toward synthesis of cobalt nanoparticles.

Results

Fe3+-reducing assay demonstrated that Lantana camara methanol extract (LCM) has significant electron transfer potential. Gas chromatography mass spectroscopy (GC–MS) analysis of the crude extracts revealed the presence of 7 known and 17 unknown phytochemicals in LCM. Synthesis of cobalt nanoparticles was confirmed based on color change of reaction mixture from light brown to dark brown. UV–visible spectrometry analysis showed that the synthesized particles had a λmax at 267.5 nm. Based on the two theta (2θ) and Miller indices (hkl) values obtained in XRD analysis, the particles were confirmed to be cobalt hydroxide (Co(OH)2) nanoparticles. Further dynamic light scattering (DLS) analysis showed that the average size of the Co(OH)2 nanoparticles is 180 nm. SEM image analysis of the particles revealed that they are spherical mass of feather-like structure, contributing toward increased surface area of the particles. Further, the pharmaceutical potential of the Co(OH)2 nanoparticles was evaluated against eukaryotic cancer and fungal cells. MTT cytotoxicity analysis showed that Co(OH)2 nanoparticles have selective toxicity toward HCT-116 cancer cells with an IC50 value of 25 µg/ml and reduced cytotoxicity to non-cancerous VERO cells with an IC50 value of 200 µg/ml suggesting that the particles possess selective anti-cancerous cytotoxicity. Additionally, the particles demonstrated significant antifungal activity against 5 human fungal pathogens.

Conclusions

Results of this study conclude that green-synthesized Co(OH)2 nanoparticles using Lantanacamara L possess excellent eukaryotic cytotoxicity against cancer cells and fungal pathogens.