bluuebunny/crossref_metadata_2025 huggingface dataset:
IL-6 and SAA—Strong Predictors for the Outcome in COVID-19 CKD Patients
Authors: Filev Rumen , Lyubomirova Mila , Bogov Boris , Kalinov Krassimir , Hristova Julieta , Svinarov Dobrin , Rostaing Lionel
Published: December 2023

In this prospective study, we assessed biomarkers of inflammation (IL-6 and SAA) from the serum of 120 COVID-19 patients, of whom 70 had chronic kidney disease. All the samples were taken at emergency-department (ED) admission. Our goal was to relate the biomarkers to the results of death and acute kidney injury. All the patients underwent chest computer tomography to estimate the severity score (0–5), which was performed at hospital admission. Finally, biomarkers were also evaluated in a healthy control group and in non-COVID-19-CKD patients. IL-6 and SAA were statistically different between the subgroups, i.e., they were significantly increased in patients with COVID-19. Both of the biomarkers (IL-6 and SAA) were independently associated with mortality, AKI and a higher grade of pathological changes in the lung’s parenchyma. Both high baseline levels of IL-6 and SAA on hospital admission were highly correlated with a later ventilatory requirement and mortality, independent of hospital stay. Mortality was found to be significantly higher when the chest CT severity score was 3–4, compared with a severity score of 0–2 (p < 0.0001). Conclusions: at the admission stage, IL-6 and SAA are useful markers for COVID-19 patients with CKD.
Glycolysis Reprogramming in Idiopathic Pulmonary Fibrosis: Unveiling the Mystery of Lactate in the Lung
Authors: Yan Peishuo , Liu Jingyi , Li Zhenwei , Wang Jiawei , Zhu Zhao , Wang Lan , Yu Guoying
Published: December 2023

Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive lung disease characterized by excessive deposition of fibrotic connective tissue in the lungs. Emerging evidence suggests that metabolic alterations, particularly glycolysis reprogramming, play a crucial role in the pathogenesis of IPF. Lactate, once considered a metabolic waste product, is now recognized as a signaling molecule involved in various cellular processes. In the context of IPF, lactate has been shown to promote fibroblast activation, myofibroblast differentiation, and extracellular matrix remodeling. Furthermore, lactate can modulate immune responses and contribute to the pro-inflammatory microenvironment observed in IPF. In addition, lactate has been implicated in the crosstalk between different cell types involved in IPF; it can influence cell–cell communication, cytokine production, and the activation of profibrotic signaling pathways. This review aims to summarize the current research progress on the role of glycolytic reprogramming and lactate in IPF and its potential implications to clarify the role of lactate in IPF and to provide a reference and direction for future research. In conclusion, elucidating the intricate interplay between lactate metabolism and fibrotic processes may lead to the development of innovative therapeutic strategies for IPF.
Prospective Application of Tannic Acid in Acetaminophen (APAP)-Induced Acute Liver Failure
Authors: Lin Yong-Heng , Lin Yu-Che , Hou Yung-Te
Published: December 2023

This study investigated the effect of tannic acid (TA), a natural plant-derived polyphenol, on hepatocyte viability and function, focusing on both hepatoprotective and hepatocurative aspects within liver failure models. In an in vitro prevention model, the TA-containing group exhibited 1.5-fold and 59-fold higher relative cell viability and albumin synthesis, respectively, in injured mature hepatocytes (MHs) and 1.14-fold and 1.10-fold higher values in injured small hepatocytes (SHs), compared with the TA-free group. In the in vitro curative model, the TA-containing group exhibited 3.25-fold and 113-fold higher relative cell viability and albumin synthesis, respectively, in injured MHs and 0.36-fold and 3.55-fold higher values in injured SHs, compared with the TA-free group. In the in vivo disease model, the administration of 300 μL of 1 μg/mL TA significantly mitigated acute liver failure damage and post-APAP toxicity in mice. This was evident in serum analysis, where the levels of alanine transaminase, aspartate aminotransferase, and total bilirubin notably decreased, in agreement with histological observations. The study findings reveal that TA can enhance hepatic function at specific additive concentrations. Furthermore, even when injured by APAP, hepatocytes could revert to their preinjury state after additional TA supplementation. Additionally, pretreating hepatocytes with TA can alleviate subsequent damage. Thus, TA holds clinical potential in the treatment of APAP-induced liver failure.
Hyper-Production of Pullulan by a Novel Fungus of Aureobasidium melanogenum ZH27 through Batch Fermentation
Authors: Wang Qin-Qing , Lin Jia , Zhou Qian-Zhi , Peng Juan , Zhang Qi , Wang Jiang-Hai
Published: December 2023

Pullulan, which is a microbial exopolysaccharide, has found widespread applications in foods, biomedicines, and cosmetics. Despite its versatility, most wild-type strains tend to yield low levels of pullulan production, and their mutants present genetic instability, achieving a limited increase in pullulan production. Therefore, mining new wild strains with robust pullulan-producing abilities remains an urgent concern. In this study, we found a novel strain, namely, Aureobasidium melanogenum ZH27, that had a remarkable pullulan-producing capacity and optimized its cultivation conditions using the one-factor-at-a-time method. To elucidate the reasons that drove the hyper-production of pullulan, we scrutinized changes in cell morphology and gene expressions. The results reveal that strain ZH27 achieved 115.4 ± 1.82 g/L pullulan with a productivity of 0.87 g/L/h during batch fermentation within 132 h under the optimized condition (OC). This pullulan titer increased by 105% compared with the initial condition (IC). Intriguingly, under the OC, swollen cells featuring 1–2 large vacuoles predominated during a rapid pullulan accumulation, while these swollen cells with one large vacuole and several smaller ones were prevalent under the IC. Moreover, the expressions of genes associated with pullulan accumulation and by-product synthesis were almost all upregulated. These findings suggest that swollen cells and large vacuoles may play pivotal roles in the high level of pullulan production, and the accumulation of by-products also potentially contributes to pullulan synthesis. This study provides a novel and promising candidate for industrial pullulan production.
Analysis of Chromatin Accessibility and DNA Methylation to Reveal the Functions of Epigenetic Modifications in Cyprinus carpio Gonads
Authors: Hou Mingxi , Wang Qi , Zhao Ran , Cao Yiming , Zhang Jin , Sun Xiaoqing , Yu Shuangting , Wang Kaikuo , Chen Yingjie , Zhang Yan , Li Jiongtang
Published: December 2023

Epigenetic modifications are critical in precisely regulating gene expression. The common carp (Cyprinus carpio) is an economically important fish species, and females exhibit faster growth rates than males. However, the studies related to epigenetic modifications in the common carp gonads are limited. In this study, we conducted the Assay for Transposase Accessible Chromatin sequencing (ATAC-seq) and Bisulfite sequencing (BS-seq) to explore the roles of epigenetic modifications in the common carp gonads. We identified 84,207 more accessible regions and 77,922 less accessible regions in ovaries compared to testes, and some sex-biased genes showed differential chromatin accessibility in their promoter regions, such as sox9a and zp3. Motif enrichment analysis showed that transcription factors (TFs) associated with embryonic development and cell proliferation were heavily enriched in ovaries, and the TFs Foxl2 and SF1 were only identified in ovaries. We also analyzed the possible regulations between chromatin accessibility and gene expression. By BS-seq, we identified 2087 promoter differentially methylated genes (promoter-DMGs) and 5264 gene body differentially methylated genes (genebody-DMGs) in CG contexts. These genebody-DMGs were significantly enriched in the Wnt signaling pathway, TGF-beta signaling pathway, and GnRH signaling pathway, indicating that methylation in gene body regions could play an essential role in sex maintenance, just like methylation in promoter regions. Combined with transcriptomes, we revealed that the expression of dmrtb1-like, spag6, and fels was negatively correlated with their methylation levels in promoter regions. Our study on the epigenetic modifications of gonads contributes to elucidating the molecular mechanism of sex differentiation and sex maintenance in the common carp.
Thiazolidin-4-Ones as a Promising Scaffold in the Development of Antibiofilm Agents—A Review
Authors: Trotsko Nazar
Published: December 2023

Thiazolidin-4-ones have a broad range of medical and clinical implementation, which is important for pharmaceutical and medicinal chemistry. This heterocyclic core has been reported to possess a diversity of bioactivities, including antimicrobial and antibiofilm-forming potential. The resistance of biofilms to antibiotics or disinfectants is a serious medical problem. Therefore, there is a natural need to discover new effective structures with properties that inhibit biofilm formation. This review aims to analyze the antibiofilm features of thiazolidin-4-ones described in the literature over the last two decades. The information gathered in this review could benefit the rational design of new effective antibiofilm small molecules with thiazolidin-4-one cores.
Molecular Therapies in Cardiovascular Diseases: Small Interfering RNA in Atherosclerosis, Heart Failure, and Hypertension
Authors: Sarzani Riccardo , Spannella Francesco , Di Pentima Chiara , Giulietti Federico , Landolfo Matteo , Allevi Massimiliano
Published: December 2023

Small interfering RNA (siRNA) represents a novel, fascinating therapeutic strategy that allows for selective reduction in the production of a specific protein through RNA interference. In the cardiovascular (CV) field, several siRNAs have been developed in the last decade. Inclisiran has been shown to significantly reduce low-density lipoprotein cholesterol (LDL-C) circulating levels with a reassuring safety profile, also in older patients, by hampering proprotein convertase subtilisin/kexin type 9 (PCSK9) production. Olpasiran, directed against apolipoprotein(a) mRNA, prevents the assembly of lipoprotein(a) [Lp(a)] particles, a lipoprotein linked to an increased risk of ischemic CV disease and heart valve damage. Patisiran, binding transthyretin (TTR) mRNA, has demonstrated an ability to improve heart failure and polyneuropathy in patients with TTR amyloidosis, even in older patients with wild-type form. Zilebesiran, designed to reduce angiotensinogen secretion, significantly decreases systolic and diastolic blood pressure (BP). Thanks to their effectiveness, safety, and tolerability profile, and with a very low number of administrations in a year, thus overcoming adherence issues, these novel drugs are the leaders of a new era in molecular therapies for CV diseases.
Editorial: Photodynamic Therapy as an Important Tool for Biological Breakthroughs—Photoactive Photosensitizers Applied from Cancer to Microbial Targets
Authors: Lourenço Leandro M. O. , Tomé Augusto C. , Tomé João P. C.
Published: December 2023

Photodynamic therapy (PDT) stands as an approved clinical treatment for both oncologic and nononcologic disorders [...]
1,3-Dichloroadamantyl-Containing Ureas as Potential Triple Inhibitors of Soluble Epoxide Hydrolase, p38 MAPK and c-Raf
Authors: Gladkikh Boris P. , Danilov Dmitry V. , D’yachenko Vladimir S. , Butov Gennady M.
Published: December 2023

Soluble epoxide hydrolase (sEH) is an enzyme involved in the metabolism of bioactive lipid signaling molecules. sEH converts epoxyeicosatrienoic acids (EET) to virtually inactive dihydroxyeicosatrienoic acids (DHET). The first acids are “medicinal” molecules, the second increase the inflammatory infiltration of cells. Mitogen-activated protein kinases (p38 MAPKs) are key protein kinases involved in the production of inflammatory mediators, including tumor necrosis factor-α (TNF-α) and cyclooxygenase-2 (COX-2). p38 MAPK signaling plays an important role in the regulation of cellular processes, especially inflammation. The proto-oncogenic serine/threonine protein kinase Raf (c-Raf) is a major component of the mitogen-activated protein kinase (MAPK) pathway: ERK1/2 signaling. Normal cellular Raf genes can also mutate and become oncogenes, overloading the activity of MEK1/2 and ERK1/2. The development of multitarget inhibitors is a promising strategy for the treatment of socially dangerous diseases. We synthesized 1,3-disubstituted ureas and diureas containing a dichloroadamantyl moiety. The results of computational methods show that soluble epoxide hydrolase inhibitors can act on two more targets in different signaling pathways of mitogen-activated protein kinases p38 MAPK and c-Raf. The two chlorine atoms in the adamantyl moiety may provide additional Cl-π interactions in the active site of human sEH. Molecular dynamics studies have shown that the stability of ligand–protein complexes largely depends on the “spacer effect.” The compound containing a bridge between the chloroadamantyl fragment and the ureide group forms more stable ligand–protein complexes with sEH and p38 MAPK, which indicates a better conformational ability of the molecule in the active sites of these targets. In turn, a compound containing two chlorine atoms forms a more stable complex with c-Raf, probably due to the presence of additional halogen bonds of chlorine atoms with amino acid residues.
COPI Vesicle Disruption Inhibits Mineralization via mTORC1-Mediated Autophagy
Authors: Nie Jiaming , Ma Shaoyang , Zhang Yuchen , Yu Shuchen , Yang Jiajia , Li Ang , Pei Dandan
Published: December 2023

Bone mineralization is a sophisticated regulated process composed of crystalline calcium phosphate and collagen fibril. Autophagy, an evolutionarily conserved degradation system, whereby double-membrane vesicles deliver intracellular macromolecules and organelles to lysosomes for degradation, has recently been shown to play an essential role in mineralization. However, the formation of autophagosomes in mineralization remains to be determined. Here, we show that Coat Protein Complex I (COPI), responsible for Golgi-to-ER transport, plays a pivotal role in autophagosome formation in mineralization. COPI vesicles were increased after osteoinduction, and COPI vesicle disruption impaired osteogenesis. Mechanistically, COPI regulates autophagy activity via the mTOR complex 1 (mTORC1) pathway, a key regulator of autophagy. Inhibition of mTOR1 rescues the impaired osteogenesis by activating autophagy. Collectively, our study highlights the functional importance of COPI in mineralization and identifies COPI as a potential therapeutic target for treating bone-related diseases.
Administration of Bevacizumab and the Risk of Chronic Kidney Disease Development in Taiwan Residents: A Population-Based Retrospective Cohort Study
Authors: Lye Lon-Fye , Chou Ruey-Hwang , Wu Tsai-Kun , Chuang Wu-Lung , Tsai Stella Chin-Shaw , Lin Heng-Jun , Tsai Fuu-Jen , Chang Kuang-Hsi
Published: December 2023

Vascular endothelial growth factor (VEGF) plays a significant role as a pro-angiogenic and pro-permeability factor within the kidney. Bevacizumab is a pharmaceutical monoclonal anti-VEGF antibody that inhibits the growth of new blood vessels, which blocks blood supply and thereby restricts tumor growth. Thus, we conducted a nationwide study to explore the risk of chronic kidney disease (CKD) development in Taiwan residents after bevacizumab therapy. We drew data from the extensive National Health Insurance Research Database (NHIRD), which encompasses data from >99% of Taiwan’s population from 1995 onwards. Individuals who received bevacizumab between 2012–2018 were identified as the bevacizumab cohort, with the index date set at the first usage. We randomly selected dates within the study period for the control group to serve as index dates. We excluded patients with a history of CKD prior to the index date or those <20 years old. In both cohorts, patients’ propensity scores matched in a 1:1 ratio based on sex, age, index year, income, urbanization level, comorbidities, and medications. We found patients treated with bevacizumab had a significantly higher risk of contracting CKD than patients without bevacizumab (adjusted hazard ratio = 1.35, 95% confidence interval = 1.35–1.73). The risk of CKD was 1.35-fold higher in participants with bevacizumab treatment than those in the control group. These findings suggest that close monitoring of CKD development after bevacizumab administration is needed.
Recombinant Phytase Modulates Blood Amino Acids and Proteomics Profiles in Pigs Fed with Low-Protein, -Calcium, and -Phosphorous Diets
Authors: Shili Cedrick N. , Kiyimba Frank , Hartsen Steve , Ramanathan Ranjith , Pezeshki Adel
Published: December 2023

A beneficial effect of corn-expressed phytase (CEP) on the growth performance of pigs fed with very low-protein (VLP) diets was previously shown. Little is known whether this improvement is related to alterations in the expression profiles of blood proteins and amino acids (AAs). The objective of this study was to investigate whether supplementation of VLP, low-calcium (Ca), and low-P diets with a CEP would alter the blood AAs and protein expression profiles in pigs. Forty-eight pigs were subjected to one of the following groups (n = 8/group) for 4 weeks: positive control (PC), negative control-reduced protein (NC), NC + low-dose CEP (LD), NC + high-dose CEP (HD), LD with reduced Ca/P (LDR), and HD with reduced Ca/P (HDR). Plasma leucine and phenylalanine concentrations were reduced in NC; however, the LD diet recovered the concentration of these AAs. Serum proteomics analysis revealed that proteins involved with growth regulation, such as selenoprotein P were upregulated while the IGF-binding proteins family proteins were differentially expressed in CEP-supplemented groups. Furthermore, a positive correlation was detected between growth and abundance of proteins involved in bone mineralization and muscle structure development. Taken together, CEP improved the blood profile of some essential AAs and affected the expression of proteins involved in the regulation of growth.
Prognostic Value of the Width of Invasion in pT3 Cutaneous Melanomas
Authors: Țăpoi Dana Antonia , Gheorghișan-Gălățeanu Ancuța-Augustina , Gosman Laura Maria , Dumitru Adrian Vasile , Ciongariu Ana Maria , Costache Mariana
Published: December 2023

Intermediate-thickness melanomas display highly variable outcomes influenced by both clinical and histopathological characteristics. This study investigates several clinicopathological prognostic factors for pT3 cutaneous melanomas, focusing on a novel parameter, the width of invasion. This is a retrospective study of 49 patients diagnosed with cutaneous melanoma between 2012 and 2018 who were followed up for at least five years. We evaluated the age, gender, tumor location, Breslow depth of invasion, width of invasion, mitotic index, the presence/absence of ulceration, regression, microsatellites, lymphovascular invasion, and perineural invasion for their association with disease progression and survival. Cox univariate analysis revealed that progression-free survival (PFS) was significantly associated with age, depth of invasion, width of invasion, lymphovascular invasion, microsatellites, and perineural invasion. Overall survival (OS) was significantly associated with age, depth of invasion, width of invasion, microsatellites, and perineural invasion. Through multivariate Cox proportional hazards regression, the only factor associated with both PFS and OS was the width of the invasion. This is one of the few studies to assess the width of invasion and we have demonstrated that this parameter could become an important prognostic factor for cutaneous melanomas.
Safety and Performance of the Subcutaneous Implantable Cardioverter Defibrillator Detection Algorithm INSIGHTTM in Pacemaker Patients
Authors: Weipert Kay F. , Kostic Srdjan , Gökyildirim Timur , Johnson Victoria , Chasan Ritvan , Gemein Christopher , Rosenbauer Josef , Erkapic Damir , Schmitt Jörn
Published: December 2023

Background: The use of the S-ICD is limited by its inability to provide backup pacing. Combined use of the S-ICD with a pacemaker may be a good choice in certain situations, yet current experience concerning the compatibility is limited. The goal of this study was to determine the safety and efficacy of the S-ICD in patients with a pacemaker. Methods: A total of 74 consecutive patients with a bipolar pacemaker were prospectively enrolled. First, surface rhythm strips were recorded in all possible pacemaker stimulation modes, to screen for T-wave oversensing (TWOS). Second, a S-ICD functional dummy was placed epicutaneously on the patient in the typical implant position. The same standardized pacing protocol was used as mentioned above, and every stimulation mode was recorded via S-ECG in all vectors. Results: In 16 patients (21.6%), programmed stimulation would have led to VT/VF detection. Triggered episodes were due to counting of the pacing spike(s), QRS complex, premature ventricular contractions, and/or additional TWOS. Three cases triggered in the bipolar stimulation mode. Oversensing was associated with lung emphysema and a reduced QRS amplitude in the S-ECG. Conclusion: The combination of an S-ICD and a pacemaker may lead to inadequate shock delivery due to oversensing, even under programmed bipolar stimulation. Oversensing cannot be sufficiently predicted by the screening tool in pacemaker patients. Testing with an epicutaneous S-ICD dummy in all vectors and stimulation settings is recommended in patients with pre-existing pacemakers.
Linear Longitudinal Strength Analysis of a Multipurpose Cargo Ship under Combined Bending and Torsional Load
Authors: Abedin Joynal , Franklin Francis , Mahmud S. M. Ikhtiar
Published: December 2023

Cargo ships with wide hatches usually have thin walls and limited torsional rigidity. Consequently, conducting a comprehensive torsional analysis is important because these loads can exert a significant impact. In this paper, the structural response of a multipurpose cargo ship to combined bending and torsional loads is studied using finite element analysis. The bending and torsional moments are calculated following the rules and standard regulations followed by the classification society. The ship’s 3D finite element model was verified using beam theory and direct calculations. In contrast, the accuracy of torsional stress was confirmed by comparing thin wall girder theory with direct calculation results. This study thoroughly examined the impacts of the still water bending moment, the vertical wave bending moment, and the wave-induced torsional moment on the structural response of ships. Furthermore, it scrutinised the impact of torsion on both open-deck and closed-deck ships. Hull girder normal stresses at midship due to still water and the vertical wave bending moment are shown to contribute to almost 70% of total stress in an inclined condition; stresses resulting from the horizontal wave bending moment contribute nearly 10%, while warping stresses contribute approximately 20% in open-deck ships. It is also shown that torsion has little impact on closed-deck ships. Finally, a buckling analysis was conducted to assess the ship’s buckling criteria, confirming that the linear buckling criteria were satisfied.
Regulatory T Cells in Multiple Sclerosis Diagnostics—What Do We Know So Far?
Authors: Arneth Borros
Published: December 2023

Background: Multiple sclerosis (MS) is an autoimmune disorder that affects the central nervous system (CNS) through inflammation. MS symptoms become acute if the disease progresses to the relapsing phase. Aim: This review aimed to evaluate the role played by regulatory T cells (Tregs) in the pathogenesis of MS. Methods: This review used scholarly journal articles obtained from PubMed, PsycINFO, and CINAHL with different search parameters such as ‘regulatory T cells’, ‘multiple sclerosis’, and ‘current knowledge’. The process of searching for articles was limited to those that had publication dates falling between 2010 and 2020. Results: Tregs play a role in the pathogenesis of MS. This conclusion is supported by animal disease models and environmental factors that can underlie Treg alterations in MS. Despite the knowledge of the role played by Tregs in MS pathogenesis, the specific subsets of Tregs involved in MS development remain incompletely understood. Discussion: This review provides an essential link between Tregs and MS activity. Targeting Tregs could be an efficient way to establish new treatment methods for MS management. Conclusion: MS is a complex condition affecting many people worldwide. Research has shown that Tregs can influence MS development and progression. More investigations are needed to understand how Tregs affect the pathogenesis of MS.
Introduction: Fairy Tales and Other Horrors
Authors: Tosi Laura , Cabiati Alessandro
Published: December 2023

In a Christmas 2017 interview with the British magazine Fortean Times, the celebrated Mexican filmmaker Guillermo del Toro described ‘Hansel and Gretel’, ‘the original Cinderella’, and ‘Little Red Riding Hood’ as ‘a horror story’, before affirming that ‘horror and the fairy tale walk hand in hand’ (del Toro 2017, p [...]
Effect of Local Pressurization on Microstructure and Mechanical Properties of Aluminum Alloy Flywheel Housing with Complex Shape
Authors: Chen Qiang , Ge Ning , Jiang Jufu , Huang Minjie , Li Mingxing , Wang Ying , Dong Jian , Ding Changjie , Zou Dechao
Published: December 2023

In this work, squeeze casting experiments of flywheel housing components with a large wall thickness difference and a complex shape were carried out with AlSi9Mg aluminum alloy. The defects, microstructures, and mechanical properties under different process parameters were investigated. Furthermore, the local pressurization process was applied to the thick-walled positions to force-feed the cast defects. The mechanical properties and microstructures at these positions were analyzed. The results showed that the surface quality of formed components was good and that local pressurization could effectively reduce the shrinkage cavity and shrinkage porosity in thick walls, but the scope and effect of forced feeding were limited. The optimum process parameters were a pouring temperature of 650 °C, a specific pressure of 48 MPa, a mold temperature of 220 °C, a local pressurization of 800 MPa, and pressure delay times of 15 s (side A) and 17 s (side B). The ultimate tensile strength, yield strength, and elongation of the formed component under validation experiments of the optimum process parameters were 201 MPa, 103 MPa, and 5.1%. Meanwhile, the fine grains of primary α-Al were mainly rosette and equiaxed grains, and the average grain size was about 40 μm. The microstructure of the eutectic silicon was acicular and was prone to segregation under pressure. According to profile morphology, the positions after pressurization were divided into a deformation zone, a direct action zone, and an indirect action zone. The coexistence of as-cast and plastic deformation microstructures was observed. The effect of local pressurization mainly involved a change in the solidification process, plastic deformation, and forced feeding.
A Micromechanical Study of Interactions of Cyanate Ester Monomer with Graphene or Boron Nitride Monolayer
Authors: Sachdeva Geeta , Lobato Álvaro , Pandey Ravindra , Odegard Gregory M.
Published: December 2023

Polymer composites, hailed for their ultra-strength and lightweight attributes, stand out as promising materials for the upcoming era of space vehicles. The selection of the polymer matrix plays a pivotal role in material design, given its significant impact on bulk-level properties through the reinforcement/polymer interface. To aid in the systematic design of such composite systems, molecular-level calculations are employed to establish the relationship between interfacial characteristics and mechanical response, specifically stiffness. This study focuses on the interaction of fluorinated and non-fluorinated cyanate ester monomers with graphene or a BN monolayer, representing non-polymerized ester composites. Utilizing micromechanics and the density functional theory method to analyze interaction energy, charge density, and stiffness, our findings reveal that the fluorinated cyanate-ester monomer demonstrates lower interaction energy, reduced pull-apart force, and a higher separation point compared to the non-fluorinated counterpart. This behavior is attributed to the steric hindrance caused by fluorine atoms. Furthermore, the BN monolayer exhibits enhanced transverse stiffness due to increased interfacial strength, stemming from the polar nature of B–N bonds on the surface, as opposed to the C-C bonds of graphene. These molecular-level results are intended to inform the design of next-generation composites incorporating cyanate esters, specifically for structural applications.
Investigation on the Attainment of High-Density 316L Stainless Steel with Selective Laser Sintering
Authors: Zhu Pengfei , He Xinbo , Guan Hongda , Zhang Zijian , Zhang Tao , Qu Xuanhui
Published: December 2023

Due to the low density of the green part produced by selective laser sintering (SLS), previous reports mainly improve the sample’s density through the infiltration of low-melting metals or using isostatic pressing technology. In this study, the feasibility of preparing high-density 316L stainless steel using 316L and epoxy resin E-12 as raw materials for SLS combined with debinding and sintering was investigated. The results indicated that in an argon atmosphere, high carbon and oxygen contents, along with the uneven distribution of oxygen, led to the formation of impurity phases such as metal oxides, including Cr2O3 and FeO, preventing the effective densification of the sintered samples. Hydrogen-sintered samples can achieve a high relative density exceeding 98% without losing their original design shape. This can be attributed to hydrogen’s strong reducibility (effectively reducing the carbon and oxygen contents in the samples, improving their distribution uniformity, and eliminating impurity phases) and hydrogen’s higher thermal conductivity (about 10 times that of argon, reducing temperature gradients in the sintered samples and promoting better sintering). The microstructure of the hydrogen-sintered samples consisted of equiaxed austenite and ferrite phases. The samples exhibited the highest values of tensile strength, yield strength, and elongation at 1440 °C, reaching 513.5 MPa, 187.4 MPa, and 76.1%, respectively.
Influence of Fly Ash Content on the Durability of Mortar Specimens under Dry/Wet Sulfate Attack
Authors: Zhang Yage , Wu Dongge , Wang Yushan , Zhou Yang , Wang Shan , Zhao Yi
Published: December 2023

To investigate the durability of cementitious materials under complex environmental conditions in Xinjiang, this study conducted durability tests on mortar specimens with different fly ash contents under dry/wet sulfate attack conditions, with standard curing and steam curing at 70 °C. The appearance loss and flexural and compressive strength variations in the specimens were analyzed, and an evolution model of the mortar strength under a dry/wet sulfate attack was established. Moreover, XRD and SEM techniques were used to characterize the erosion products and microstructure, and to explore the erosion resistance mechanism of fly ash cementitious materials. The results showed that, after 160 cycles of erosion, the flexural strength of the specimens decreased with the increase in the fly ash content. In the context of steam-cured mortar specimens, throughout the entire erosion period, specimens with a fly ash content of 45% exhibited the highest relative compressive strength. The established strength evolution model had a minimum determination coefficient of 0.879, indicating a good agreement between the model and experimental results. Microscopic research showed that fly ash would undergo a pozzolanic reaction under the action of sulfate and calcium hydroxide, which was beneficial to the improvement of the erosion resistance. As the fly ash content increased, the erosion products of the specimens gradually became dominated by gypsum.
Biomedical Applications of Titanium Alloys: A Comprehensive Review
Authors: Marin Elia , Lanzutti Alex
Published: December 2023

Titanium alloys have emerged as the most successful metallic material to ever be applied in the field of biomedical engineering. This comprehensive review covers the history of titanium in medicine, the properties of titanium and its alloys, the production technologies used to produce biomedical implants, and the most common uses for titanium and its alloys, ranging from orthopedic implants to dental prosthetics and cardiovascular devices. At the core of this success lies the combination of machinability, mechanical strength, biocompatibility, and corrosion resistance. This unique combination of useful traits has positioned titanium alloys as an indispensable material for biomedical engineering applications, enabling safer, more durable, and more efficient treatments for patients affected by various kinds of pathologies. This review takes an in-depth journey into the inherent properties that define titanium alloys and which of them are advantageous for biomedical use. It explores their production techniques and the fabrication methodologies that are utilized to machine them into their final shape. The biomedical applications of titanium alloys are then categorized and described in detail, focusing on which specific advantages titanium alloys are present when compared to other materials. This review not only captures the current state of the art, but also explores the future possibilities and limitations of titanium alloys applied in the biomedical field.
ZrO2 Superhydrophobic Coating with an Excellent Corrosion Resistance and Stable Degradation Performance on Zr-Based Bulk Metallic Glass
Authors: Wei Ranfeng , Zheng Rui , Li Chaojun , Wang Wei , Zhang Hao , Sun Qijing , Lv Jingwang , Zhang Guoyang , Liu Li , Zhao Xiangjin
Published: December 2023

Photocatalysis is an energy-saving and high-efficiency green environmental technology. Because of its wide band gap and low light utilization, few studies have been conducted on ZrO2 used as a photocatalytic material. In this paper, a corrosion-resistant superhydrophobic ZrO2 coating was prepared on the surface of Zr-based bulk metallic glass by electrochemical etching. This coating not only showed a better corrosion resistance and easier collection, but also presented a stable degradation performance when combined with H2O2; these characteristics are necessary for photocatalysts to survive under harsh environments. This study provides a new direction for designing superhydrophobic surfaces on bulk metallic glass that possess a functional performance.
Optical Pump–Terahertz Probe Diagnostics of the Carrier Dynamics in Diamonds
Authors: Bulgakova Vladislava , Chizhov Pavel , Ushakov Alexander , Ratnikov Pavel , Goncharov Yuri , Martyanov Artem , Kononenko Vitali , Savin Sergey , Golovnin Ilya , Konov Vitaly , Garnov Sergey
Published: December 2023

Diamond is a promising material for terahertz applications. In this work, we use a non-invasive optical pump–terahertz probe method to experimentally study the photoinduced carrier dynamics in doped diamond monocrystals and a new diamond-silicon composite. The chemical vapor deposited diamond substrate with embedded silicon microparticles showed two photoinduced carrier lifetimes (short lifetime on the order of 4 ps and long lifetime on the order of 200 ps). The short lifetime is several times less than in boron-doped diamonds and nitrogen-doped diamonds which were grown using a high temperature–high pressure technique. The observed phenomenon is explained by the transport of photoexcited carriers across the silicon–diamond interface, resulting in dual relaxation dynamics. The observed phenomenon could be used for ultrafast flexible terahertz modulation.
Stress Distribution in Wear Analysis of Nano-Y2O3 Dispersion Strengthened Ni-Based μm-WC Composite Material Laser Coating
Authors: Tao Li , Yang Yang , Zhu Wenliang , Sun Jian , Wu Jiale , Xu Hao , Yan Lu , Yang Anhui , Xu Zhilong
Published: December 2023

Oxide-dispersion- and hard-particle-strengthened (ODS) laser-cladded single-layer multi-tracks with a Ni-based alloy composition with 20 wt.% μm-WC particles and 1.2 wt.% nano-Y2O3 addition were produced on ultra-high-strength steel in this study. The investigation of the composite coating designed in this study focused on the reciprocating friction and wear workpiece surface under heavy load conditions. The coating specimens were divided into four groups: (i) Ni-based alloy, nano-Y2O3, and 2 μm-WC (2 μm WC-Y/Ni); (ii) Ni-based alloy with added 2 μm-WC (2 μmWC/Ni); (iii) Ni-based alloy with added 80 μm-WC (80 μmWC/Ni); and (iv) base metal ultra-high-strength alloy steel 30CrMnSiNi2A. Four conclusions were reached: (1) Nano-Y2O3 could effectively inhibit the dissolution of 2 μm-WC. (2) It can be seen from the semi-space dimensionless simulation results that the von Mises stress distribution of the metal laser composite coating prepared with a 2 μm-WC particle additive was very uniform and it had better resistance to normal impact and tangential loads than the laser coating prepared with the 80 μm-WC particle additive. (3) The inherent WC initial crack and dense stress concentration in the 80 μm-WC laser coating could easily cause dislocations to accumulate, as shown both quantitatively and qualitatively, resulting in the formation of micro-crack nucleation. After the end of the running-in phase, the COF of the 2 μm-WC-Y2O3/Ni component samples stabilized at the minimum of the COF of the four samples. The numerical order of the four COF curves was stable from small to large as follows: 2 μm-WC-Y2O3/Ni, 2 μm-WC/Ni, 80 μm-WC/Ni, and 30CrMnSiNi2A. (4) The frictional volume loss rate of 2 μm-WC-Y2O3/Ni was 1.3, which was significantly lower than the corresponding values of the other three components: 2.4, 3.5, and 13.
Rational Design of Flexible Mechanical Force Sensors for Healthcare and Diagnosis
Authors: Zhang Hang , Zhang Yihui
Published: December 2023

Over the past decade, there has been a significant surge in interest in flexible mechanical force sensing devices and systems. Tremendous efforts have been devoted to the development of flexible mechanical force sensors for daily healthcare and medical diagnosis, driven by the increasing demand for wearable/portable devices in long-term healthcare and precision medicine. In this review, we summarize recent advances in diverse categories of flexible mechanical force sensors, covering piezoresistive, capacitive, piezoelectric, triboelectric, magnetoelastic, and other force sensors. This review focuses on their working principles, design strategies and applications in healthcare and diagnosis, with an emphasis on the interplay among the sensor architecture, performance, and application scenario. Finally, we provide perspectives on the remaining challenges and opportunities in this field, with particular discussions on problem-driven force sensor designs, as well as developments of novel sensor architectures and intelligent mechanical force sensing systems.
Pestalotiopols E–J, Six New Polyketide Derivatives from a Marine Derived Fungus Pestalotiopsis sp. SWMU-WZ04-1
Authors: Jiang Liyuan , Teng Baorui , Zhang Mengyu , Chen Siwei , Zhang Dan , Zhai Longfei , Lin Jiafu , Lei Hui
Published: December 2023

Chemical epigenetic cultivation of the sponge-derived fungus Pestalotiopsis sp. SWMU-WZ04-1 contributed to the identification of twelve polyketide derivatives, including six new pestalotiopols E–J (1–6) and six known analogues (7–12). Their gross structures were deduced from 1D/2D NMR and HRESIMS spectroscopic data, and their absolute configurations were further established by circular dichroism (CD) Cotton effects and the modified Mosher’s method. In the bioassay, the cytotoxic and antibacterial activities of all compounds were evaluated. Chlorinated benzophenone derivatives 7 and 8 exhibited inhibitory effects on Staphylococcus aureus and Bacillus subtilis, with MIC values varying from 3.0 to 50 μg/mL. In addition, these two compounds were cytotoxic to four types of human cancer cells, with IC50 values of 16.2~83.6 μM. The result showed that compound 7 had the probability of being developed into a lead drug with antibacterial ability.
Optimized Design of an Ultrasonic-Based High-Efficiency Wireless Passive Monitoring System for Sealed Metal Compartments
Authors: Qi Bowen , Cui Juan , Zheng Yongqiu , Zhang Bingrui , Chu Chengqun , Yan Xiaolong , Gao Xiang , Xue Chenyang
Published: December 2023

The condition monitoring (CM) of sealed metal compartments (SMCs) is an urgently required restructure. Ultrasound penetrates SMCs to power and communicate with built-in sensors, enabling the CM of SMCs. However, current ultrasonic wireless power transfer and data communication (UWPTADC) systems are large and complex, and limited by the efficiency of energy transfer and data reliability. In this paper, an optimized design of a high-efficiency wireless passive monitoring system using UWPTADC techniques is proposed for SMC. The circuit model of the system is developed and analyzed to achieve an optimal design for efficient wireless power transfer and effective data communication coupling. A test system was constructed using a steel wall of 11 mm thickness as a validation object. At the ultrasonic carrier frequency of 1.045 MHz, the system has an energy transfer efficiency of 60%, and a communication rate of 50 kbps. In addition, the system realizes temperature and humidity monitoring inside a 13 mm thick cylindrical SMC, simulating the process of ultrasonic CM of an actual engine compartment. The system provides a wiring-free and battery-free solution for CM in SMCs, advancing CM in aerospace, marine and other fields.
Selenoproteins in Health
Authors: Qi Ziqi , Duan Alex , Ng Ken
Published: December 2023

Selenium (Se) is a naturally occurring essential micronutrient that is required for human health. The existing form of Se includes inorganic and organic. In contrast to the inorganic Se, which has low bioavailability and high cytotoxicity, organic Se exhibits higher bioavailability, lower toxicity, and has a more diverse composition and structure. This review presents the nutritional benefits of Se by listing and linking selenoprotein (SeP) functions to evidence of health benefits. The research status of SeP from foods in recent years is introduced systematically, particularly the sources, biochemical transformation and speciation, and the bioactivities. These aspects are elaborated with references for further research and utilization of organic Se compounds in the field of health.
Overnutrition during Pregnancy and Lactation Induces Gender-Dependent Dysmetabolism in the Offspring Accompanied by Heightened Stress and Anxiety
Authors: Melo Gonçalo M. , Capucho Adriana M. , Sacramento Joana F. , Ponce-de-Leão José , Fernandes Marcos V. , Almeida Inês F. , Martins Fátima O. , Conde Silvia V.
Published: December 2023

Maternal obesity and gestational diabetes predispose the next generation to metabolic disturbances. Moreover, the lactation phase also stands as a critical phase for metabolic programming. Nevertheless, the precise mechanisms originating these changes remain unclear. Here, we investigate the consequences of a maternal lipid-rich diet during gestation and lactation and its impact on metabolism and behavior in the offspring. Two experimental groups of Wistar female rats were used: a control group (NC) that was fed a standard diet during the gestation and lactation periods and an overnutrition group that was fed a high-fat diet (HF, 60% lipid-rich) during the same phases. The offspring were analyzed at postnatal days 21 and 28 and at 2 months old (PD21, PD28, and PD60) for their metabolic profiles (weight, fasting glycemia insulin sensitivity, and glucose tolerance) and euthanized for brain collection to evaluate metabolism and inflammation in the hypothalamus, hippocampus, and prefrontal cortex using Western blot markers of synaptic dynamics. At 2 months old, behavioral tests for anxiety, stress, cognition, and food habits were conducted. We observed that the female offspring born from HF mothers exhibited increased weight gain and decreased glucose tolerance that attenuated with age. In the offspring males, weight gain increased at P21 and worsened with age, while glucose tolerance remained unchanged. The offspring of the HF mothers exhibited elevated levels of anxiety and stress during behavioral tests, displaying decreased predisposition for curiosity compared to the NC group. In addition, the offspring from mothers with HF showed increased food consumption and a lower tendency towards food-related aggression. We conclude that exposure to an HF diet during pregnancy and lactation induces dysmetabolism in the offspring and is accompanied by heightened stress and anxiety. There was sexual dimorphism in the metabolic traits but not behavioral phenotypes.
Anti-Inflammatory Mechanisms of Curcumin and Its Metabolites in White Adipose Tissue and Cultured Adipocytes
Authors: Islam Tariful , Scoggin Shane , Gong Xiaoxia , Zabet-Moghaddam Masoud , Kalupahana Nishan S. , Moustaid-Moussa Naima
Published: December 2023

The plant-derived polyphenol curcumin alleviates the inflammatory and metabolic effects of obesity, in part, by reducing adipose tissue inflammation. We hypothesized that the benefits of curcumin supplementation on diet-induced obesity and systemic inflammation in mice occur through downregulation of white adipose tissue (WAT) inflammation. The hypothesis was tested in adipose tissue from high-fat diet-induced obese mice supplemented with or without curcumin and in 3T3-L1 adipocytes treated with or without curcumin. Male B6 mice were fed a high-fat diet (HFD, 45% kcal fat) with or without 0.4% (w/w) curcumin supplementation (HFC). Metabolic changes in these mice have been previously reported. Here, we determined the serum levels of the curcumin metabolites tetrahydrocurcumin (THC) and curcumin-O-glucuronide (COG) using mass spectrometry. Moreover, we determined interleukin 6 (IL-6) levels and proteomic changes in LPS-stimulated 3T3-L1 adipocytes treated with or without curcumin by using immunoassays and mass spectrometry, respectively, to gain further insight into any altered processes. We detected both curcumin metabolites, THC and COG, in serum samples from the curcumin-fed mice. Both curcumin and its metabolites reduced LPS-induced adipocyte IL-6 secretion and mRNA levels. Proteomic analyses indicated that curcumin upregulated EIF2 and mTOR signaling pathways. Overall, curcumin exerted anti-inflammatory effects in adipocytes, in part by reducing IL-6, and these effects may be linked to the upregulation of the mTOR signaling pathway, warranting additional mechanistic studies on the effects of curcumin and its metabolites on metabolic health.