bluuebunny/crossref_metadata_2025 huggingface dataset:
Epigenetic Silencing of P-Element Reporter Genes Induced by Transcriptionally Active Domains of Constitutive Heterochromatin in Drosophila melanogaster
Authors: Messina Giovanni , Celauro Emanuele , Marsano Renè Massimiliano , Prozzillo Yuri , Dimitri Patrizio
Published: December 2022

Reporter genes inserted via P-element integration into different locations of the Drosophila melanogaster genome have been routinely used to monitor the functional state of chromatin domains. It is commonly thought that P-element-derived reporter genes are subjected to position effect variegation (PEV) when transposed into constitutive heterochromatin because they acquire heterochromatin-like epigenetic modifications that promote silencing. However, sequencing and annotation of the D. melanogaster genome have shown that constitutive heterochromatin is a genetically and molecularly heterogeneous compartment. In fact, in addition to repetitive DNAs, it harbors hundreds of functional genes, together accounting for a significant fraction of its entire genomic territory. Notably, most of these genes are actively transcribed in different developmental stages and tissues, irrespective of their location in heterochromatin. An open question in the genetic and molecular studies on PEV in D. melanogaster is whether functional heterochromatin domains, i.e., heterochromatin harboring active genes, are able to silence reporter genes therein transposed or, on the contrary, can drive their expression. In this work, we provide experimental evidence showing that strong silencing of the Pw+ reporters is induced even when they are integrated within or near actively transcribed loci in the pericentric regions of chromosome 2. Interestingly, some Pw+ reporters were found insensitive to the action of a known PEV suppressor. Two of them are inserted within Yeti, a gene expressed in the deep heterochromatin of chromosome 2 which carries active chromatin marks. The difference sensitivity to suppressors-exhibited Pw+ reporters supports the view that different epigenetic regulators or mechanisms control different regions of heterochromatin. Together, our results suggest that there may be more complexity regarding the molecular mechanisms underlying PEV.
Heterogeneous Genomic Divergence Landscape in Two Commercially Important European Scallop Species
Authors: Vendrami David L. J. , Hoffman Joseph I. , Wilding Craig S.
Published: December 2022

Two commercially important scallop species of the genus Pecten are found in Europe: the north Atlantic Pecten maximus and the Mediterranean Pecten jacobaeus whose distributions abut at the Almeria–Orán front. Whilst previous studies have quantified genetic divergence between these species, the pattern of differentiation along the Pecten genome is unknown. Here, we mapped RADseq data from 235 P. maximus and 27 P. jacobaeus to a chromosome-level reference genome, finding a heterogeneous landscape of genomic differentiation. Highly divergent genomic regions were identified across 14 chromosomes, while the remaining five showed little differentiation. Demographic and comparative genomics analyses suggest that this pattern resulted from an initial extended period of isolation, which promoted divergence, followed by differential gene flow across the genome during secondary contact. Single nucleotide polymorphisms present within highly divergent genomic regions were located in areas of low recombination and contrasting patterns of LD decay were found between the two species, hinting at the presence of chromosomal inversions in P. jacobaeus. Functional annotations revealed that highly differentiated regions were enriched for immune-related processes and mRNA modification. While future work is necessary to characterize structural differences, this study provides new insights into the speciation genomics of P. maximus and P. jacobaeus.
Effect of Pressure Perturbations on CO2 Degassing in a Mofette System: The Case of Hartoušov, Czech Republic
Authors: Woith Heiko , Vlček Josef , Vylita Tomáš , Dahm Torsten , Fischer Tomáš , Daskalopoulou Kyriaki , Zimmer Martin , Niedermann Samuel , Stammeier Jessica A. , Turjaková Veronika , Lanzendörfer Martin
Published: December 2022

Mofettes are gas emission sites where high concentrations of CO2 ascend through conduits from as deep as the mantle to the Earth’s surface and as such provide direct windows to processes at depth. The Hartoušov mofette, located at the western margin of the Eger Graben, is a key site to study interactions between fluids and swarm earthquakes. The mofette field (10 mofettes within an area of 100 m × 500 m and three wells of 28, 108, and 239 m depth) is characterized by high CO2 emission rates (up to 100 t/d) and helium signatures with (3He/4He)c up to 5.8 Ra, indicating mantle origin. We compiled geological, geophysical, geochemical, and isotopic data to describe the mofette system. Fluids in the Cheb basin are mixtures between shallow groundwater and brine (>40 g/L at a depth of 235 m) located at the deepest parts of the basin fillings. Overpressured CO2-rich mineral waters are trapped below the mudstones and clays of the sealing Cypris formation. Drilling through this sealing layer led to blow-outs in different compartments of the basin. Pressure transients were observed related to natural disturbances as well as human activities. External (rain) and internal (earthquakes) events can cause pressure transients in the fluid system within hours or several days, lasting from days to years and leading to changes in gas flux rates. The 2014 earthquake swarm triggered an estimated excess release of 175,000 tons of CO2 during the following four years. Pressure oscillations were observed at a wellhead lasting 24 h with increasing amplitudes (from 10 to 40 kPa) and increasing frequencies reaching five cycles per hour. These oscillations are described for the first time as a potential natural analog to a two-phase pipe–relief valve system known from industrial applications.
Sophistry and Law: The Antilogical Pattern of Judicial Debate
Authors: Giombini Stefania
Published: December 2022

This essay aims to reveal the relationship between sophistry and law in a twofold direction: on one side, how the development of ancient Greek law influenced sophistry’s production, and on the other, how and to what extent the knowledge and skills developed by sophists contributed to the development of legal expertise in classical Athens. The essay will initially focus on the historiographical category of the sophists to identify a line that connects these intellectuals to the new vision of society, the democratic polis, and the community that presides over legal and judicial life. This section will show that we can indeed speak of a “sophistic movement” in light of the structuring role of antilogies (antilogiae, or antithetical arguments) in forensic rhetoric. The rest of the essay will examine, from a theoretical point of view, sophistic methods of argument that contributed to the development of ancient Greek law. Touching on the issues of opposition, the debate, the reductio ad absurdum, and the principle of non-contradiction, the essay will highlight the relevance of sophistic thought to the judicial field and, more generally, the legal arena, in ancient Athens, so much so that one can think of the sophists as advocates of a particular legal culture.
How Differential Dimensions of Social Media Overload Influences Young People’s Fatigue and Negative Coping during Prolonged COVID-19 Pandemic? Insights from a Technostress Perspective
Authors: Pang Hua , Ji Min , Hu Xiang
Published: December 2022

Although social networking sites have emerged as the primary source of information for young people, there is a dearth of knowledge concerning the underlying associations between differential aspects of social media overload and whether social media overload ultimately influenced people’s negative coping strategies during the prolonged COVID-19 pandemic. In order to fill this gap in existing knowledge, the current research employed the stressor–strain–outcome (SSO) theoretical paradigm to explicate social media fatigue and negative coping strategies from a technostress perspective. The study used cross-sectional methodology, whereby 618 valid questionnaire responses were gathered from WeChat users to assess the conceptual model. The obtained outcomes demonstrated that information overload and communication overload positively impacted young people’s fatigue. Furthermore, these two patterns of perceived overload heighten social media fatigue, which ultimately leads to young people’s negative coping with the COVID-19 pandemic. These findings would extend the present social media fatigue and technical stress literature by identifying the value of the SSO theoretical approach in interpreting young people’s negative coping phenomena in the post-pandemic time.
Foot Reflexology: Recent Research Trends and Prospects
Authors: Cai Deng-Chuan , Chen Ching-Yun , Lo Ting-Yun
Published: December 2022

Foot reflexology is a non-invasive complementary therapy that is increasingly being accepted by modern people in recent years. To understand the research trends and prospects of foot reflexology in the past 31 years, this study used the Web of Science core collection as the data source and two visualization tools, COOC and VOSviewer, to analyze the literature related to the field of foot reflexology from 1991 to 2021. This study found that the number of articles published in the field of foot reflexology has been increasing year by year, and the top three journals with the most articles are Complementary Therapies in Clinical Practice, Therapies in Medicine, and the Journal of Alternative and Complementary Medicine. The top three most prolific authors are Wyatt, Sikorskii, and Victorson, and the core institutions in the field of foot reflexology are Michigan State University, Northwestern University, Tehran University of Medical Sciences, and the University of Exeter. Foot reflexology has been shown to have a moderating effect on anxiety, fatigue, and cancer, and is a topic of ongoing and future research. This study uses this bibliometric analysis of foot reflexology literature to provide an overview of prior knowledge and a reference direction for modern preventive medicine.
IoT Based Expert System for Diabetes Diagnosis and Insulin Dosage Calculation
Authors: Valsalan Prajoona , Hasan Najam Ul , Farooq Umer , Zghaibeh Manaf , Baig Imran
Published: December 2022

High blood glucose levels are the defining characteristic of diabetes. Uncontrolled blood glucose levels in diabetic patients might result in mortality. As a result, there is a dire need to control blood glucose levels by constantly monitoring them and delivering the appropriate amount of insulin. However, insulin consumption is affected by several variables, including age, calorific intake, and body weight. The patient must see the doctor on a regular basis in order to determine the appropriate dose. Nonetheless, hospital facilities are finding it increasingly difficult to treat patients as the number of patients rises; thus, the healthcare industry is searching for an efficient method that can alleviate their burden by assisting patients with chronic conditions through remote patient care. In this work, we have developed an expert system to provide remote treatment for diabetic patients. Our expert system consists of two distinct components: one for the patient and one for the hospital. The sole requirement for the patient will be a wearable device that captures and transmits all relevant data to the cloud. On the hospital side, there should be a system in place to process that data in the cloud. The system employs a fuzzy system to handle data in two stages. A fuzzy system is initially employed to identify whether or not a patient is diabetic. In the second stage, a fuzzy system is utilized to determine the insulin dosage for a diabetic patient. Using sensors and the ESP8266 platform, we have developed a prototype of patient-side hardware. The MATLAB fuzzy toolbox is used for the processing part, which includes fuzzy systems, and the results of the MATLAB analysis are presented in the form of simulation results to demonstrate the accuracy of the proposed system in terms of determining insulin dosage. The results of the simulation using the fuzzy toolbox for the insulin dose of the diabetic patient are significantly close to the amount of dosage prescribed by the endocrinologist.
Parenting Sense of Competence: Psychometrics and Invariance among a Community and an At-Risk Samples of Portuguese Parents
Authors: Nunes Cristina , Ayala-Nunes Lara , Ferreira Laura Inês , Pechorro Pedro , Freitas Délia , Martins Cátia , Santos Rita
Published: December 2022

Parenting sense of competence refers to parents’ perception about their ability to perform the parenting role, one of the key dimensions in family dynamics. This construct is even more important in families at psychosocial risk, where the exercise of parenting can be more challenging. The Parenting Sense of Competence scale (PSOC) is a self-report measure that aims to assesses one’s perceived efficacy and satisfaction with parenting. In this cross-sectional and instrumental study, we aimed to examine the psychometric properties of the PSOC among a Portuguese sample of community (n = 205) and at-psychological-risk (n = 273) parents. Participants completed the PSOC, the Enrich Marital Satisfaction Scale, the Parenting Stress Index, and the Parenting Alliance Inventory. Results from confirmatory factor analysis showed that a two-factor revised model obtained the best fit, with some items being removed. Our data confirmed that the PSOC has good psychometric properties, with acceptable reliability and validity and measurement invariance across the community and the at-risk samples. Findings of this psychometric exploration provided evidence that the PSOC is a reliable measure of easy application and interpretation for assessing the perceived competence of Portuguese parents.
Radicle Emergence as Seed Vigour Test Estimates Seedling Quality of Hybrid Cucumber (Cucumis sativus L.) Cultivars in Low Temperature and Salt Stress Conditions
Authors: Demir Ibrahim , Kuzucu Canan Oztokat , Ermis Sıtkı , Öktem Güleda
Published: December 2022

This work was conducted on the use of the RE (radicle emergence) test to estimate the seedling emergence (SE) and quality (seedling shoot weight (SSW), seedling root weight (SRW) and seedling height (SH)) of twelve hybrid cucumber seed lots with high germination (>95%) at low temperature conditions, high salt conditions and a combination of the two. The percentages of RE, which is a 2 mm radicle protrusion at 25 °C in the dark, were counted between 18 h and 32 h. The seeds were sown in peat moss in trays and kept at 15 °C (low temperature stress), or salty water was applied as 100 mM NaCl at 25 °C (salt stress) for three days, and both were applied in a combined-stress environment. Seedling emergence and quality parameters were reduced by both stress environments and their combination. SE, SSW, SRW and SH values ranged between: 91–100%, 614–844 mg, 102–143 mg, 6.8–8.8 cm at low temperature; 90–98%, 598–904 mg, 101–154 mg, 6.5–7.8 cm at salt stress; and 76–92%, 464–608 mg, 97–133 mg, 5.8–6.9 cm at their combination. The RE values with great differences seen among the lots (20–28 h) were regressed with seedling emergence and quality values. RE 24 h had the highest R2 and significant values in all stress environments as R2 = 0.596–0.858, p < 0.05–0.001 at low temperature; R2 = 0.620–0.827, p < 0.05–0.001 with salt; and R2 = 0.686–0.842, p < 0.05–0.001 with combined stresses. We concluded that RE as a vigour test can be used successfully to estimate the seedling quality of highly germinating hybrid cucumber seed lots. Use of the RE test in hybrid cucumber seeds and its influence on high-quality seedling production were discussed.
Hairy Root Induction of Taxus baccata L. by Natural Transformation with Rhizobium rhizogenes
Authors: He Junou , Plácido João Paulo Alves , Pateraki Irini , Kampranis Sotirios , Favero Bruno Trevenzoli , Lütken Henrik
Published: December 2022

Paclitaxel (Taxol®) is a potent anticancer agent, but the widespread pharmaceutical use of paclitaxel is hampered by its limited availability due to low accumulation levels in the native yew (Taxus spp.) plants. Currently, hairy root culture is an emerging biotechnological tool that presents several advantages such as reduced costs and higher specialized metabolite production, therefore, its application to paclitaxel production can be of commercial and medicinal interest. The objective of present study was to induce hairy root in Taxus baccata L. by transformation with the wild type Rhizobium rhizogenes A4 strain. Thus, T. baccata was inoculated by three different inoculation methods: (a) ex vitro seedlings inoculation by direct injection of a liquid bacterial culture; (b) ex vitro needles inoculation by liquid co-culturing with bacteria; (c) ex vitro shoots inoculation by dipping liquid bacterial culture. Hairy roots were formed only from ex vitro seedlings inoculated by the direct inoculation method, with transformation efficiency of 14.3%. Formation of hairy roots was observed two months after inoculation. This project forms the basis for the establishment of hairy root cultures from T. baccata for the production of paclitaxel.
Precision Management of Fruit Trees
Authors: Lo Bianco Riccardo , Pisciotta Antonino , Manfrini Luigi
Published: December 2022

The aim of the Special Issue “Precision Management of Fruit Trees” was to collect new insights to support the adoption of advanced, efficient, and sustainable management techniques in the fruit production sector [...]
Evaluation of Biogeochemical Changes in Channelized and Restored Portions of a Subtropical Floodplain
Authors: Julian Paul , Osborne Todd Z. , Ellis Rex
Published: December 2022

Floodplains are critically important ecosystems that provide a whole suite of ecosystem services, including nutrient and carbon sequestration, flood mitigation, water storage, and critical wildlife habitat. However, human modification of rivers and floodplains through channelization, artificial levee construction, reductions in the active floodplain area, and water management can significantly reduce the ecosystem function of river–floodplain systems. In this study, we evaluated the changes in the nutrient loading of the Kissimmee River floodplain during the restoration of the river–floodplain system. In addition to time-series loading analysis, we also evaluated soil nutrient concentrations across the lower portion of the Kissimmee River floodplain. During the 44-year nutrient loading time-series, the floodplain remained a nutrient exporter with changes in nutrient loading generally corresponding to both water quality (i.e., point source reductions) and hydrologic restoration activities in the watershed and Kissimmee River floodplain. During the study period, inputs of total phosphorus and total nitrogen loads from upstream either significantly increased or remained the same. In addition to external sources of nutrients, internal sources of nutrients from floodplain soils can also contribute to the total nutrient export from the system. These internal sources could be organic via the decomposition of organic matter or geologic from the original excavation of the canal and/or restoration backfilling. Soil nutrient concentrations vary between vegetative communities and landscape position and could be a significant source of phosphorus to the downstream system, which is plagued by eutrophic conditions. Therefore, as floodplain function in the Kissimmee River continues to be restored and managed, additional effort may be needed to address nutrient inputs and internal legacy nutrients.
Building Trust and Awareness to Increase AZ Native Nation Participation in COVID-19 Vaccines
Authors: Sears Grant , Tutt Marissa , Sabo Samantha , Lee Naomi , Teufel-Shone Nicolette , Baca Anthony , Bennett Marianne , Nashio J. T. Neva , Flores Fernando , Baldwin Julie
Published: December 2022

The goal of this study was to establish effective, culturally appropriate strategies to enhance participation of American Indian/Alaska Native (AI/AN) communities in prevention and treatment of COVID-19, including vaccine uptake. Thirteen Community Health Representatives (CHRs) from three Arizona Native nations tailored education materials to each community. CHRs delivered the intervention to over 160 community members and administered a pre-posttest to assess trusted sources of information, knowledge, and self-efficacy and intention regarding COVID-19 vaccines. Based on pre-posttest results, doctors/healthcare providers and CHRs were the most trusted health messengers for COVID-19 information; contacts on social media, the state and federal governments, and mainstream news were among the least trusted. Almost two-thirds of respondents felt the education session was relevant to their community and culture, and more than half reported using the education materials to talk to a family member or friend about getting vaccinated. About 67% trusted the COVID-19 information provided and 74% trusted the CHR providing the information. Culturally and locally relevant COVID-19 vaccine information was welcomed and used by community members to advocate for vaccination. The materials and education provided by CHRs were viewed as helpful and emphasized the trust and influence CHRs have in their communities.
Higher Risk for Sensitization to Commonly Consumed Herbs among Adults and Youngsters Suffering from Birch, Mugwort or Grass Pollinosis
Authors: Wagner Waldemar , Buczyłko Krzysztof , Wagner Aneta , Szwed-Kowalska Angelika , Stasiak Anna
Published: December 2022

Background: According to recent findings, mugwort and birch pollen-allergic patients represent a high-risk group for developing adverse allergic reactions to herbal spices due to cross-reacting allergens found in both pollen and raw herbs. Such associations are known as a pollen-plant food allergy syndrome. Objective: The aim of the study was to evaluate the extent of sensitization to commonly consumed herb species representing Lamiaceae, Apiaceae and Brassicaceae families in Polish patients with suspected birch, mugwort or grass pollen allergy. Materials and Methods: Data were obtained from 180 patients, adults and children with suspected allergy to aeroallergens. Skin prick tests (SPT) were performed with standard birch, mugwort, grass mixture or dust mite extracts. Prick by prick tests were performed with fresh extracts of popular herbs: basil, oregano, lemon balm, mint, salvia, rosemary, thyme, anise, caraway and mustard. Results: Twenty-nine percent of patients were characterized by concomitant positive skin prick reactions to both herbs and pollens extracts. The concomitant pollinosis significantly increased the risk of SPT reaction to all tested herbs in adults (odds ratio, OR = 2.15–7.35) and children (OR = 5.3–28). The extent of SPT responses to herbs from Lamiaceae + Apiaceae were strongly correlated with SPT responses to pollens in the pediatric group (r = 0.685/p < 0.001). Conclusion: The study demonstrates that youngsters suffering from pollinosis are at high risk of developing allergic reactions to herbs and highlights the importance of including native skin prick tests with herbs in the diagnostic work-up for suspected food allergy.
Educator-Informed Development of a Mental Health Literacy Course for School Staff: Classroom Well-Being Information and Strategies for Educators (Classroom WISE)
Authors: Semchuk Jaime C. , McCullough Shannon L. , Lever Nancy A. , Gotham Heather J. , Gonzalez Jessica E. , Hoover Sharon A.
Published: December 2022

Educators play a critical role in promoting mental health and well-being with their students. Educators also recognize that they lack knowledge and relevant learning opportunities that would allow them to feel competent in supporting student mental health. As such, educators require resources and training to allow them to develop skills in this area. The Mental Health Technology Transfer Center (MHTTC) Network partnered with the National Center for School Mental Health at the University of Maryland School of Medicine to develop Classroom Well-Being and Information for Educators (WISE), a free, three-part mental health literacy training package for educators and school staff that includes an online course, video library, and resource collection. The Classroom WISE curriculum focuses on promoting positive mental health in the classroom, as well as strategies for recognizing and responding to students experiencing mental health related distress. This paper describes the curriculum development process, including results of focus groups and key informant interviews with educators and school mental health experts. Adoption of Classroom WISE can help educators support student mental health and assist in ameliorating the youth mental health crisis.
Determinants and the Moderating Effects of Individual Characteristics on Autonomous Vehicle Adoption in China
Authors: Tang Tianpei , Wang Xiwei , Wu Jianbing , Yuan Meining , Guo Yuntao , Xu Xunqian
Published: December 2022

Along with the increasing popularity of autonomous vehicles (AVs), urban livability and public health will be enhanced due to ecofriendly issues: alleviated traffic congestion, lower car ownership, and reduced transport emissions. However, some emerging issues, including public safety, trust, privacy, reliability, underdeveloped legislation, and liability, may deter user intentions to adopt an AV. This study introduces an extensive factor, playfulness, into the technology acceptance model (TAM) to quantify the impacts of psychological factors (perceived usefulness, perceived ease of use, and perceived playfulness) on AV adoption intention. This study proposes four AV-related policy measures (financial incentivization, information dissemination, convenience, and legal normalization) and examines how policy measures motivate users to adopt an AV to facilitate public safety. Furthermore, this study investigated the moderating effects of demographic factors on the relationships between independent variables and AV adoption intention. Two models were proposed and estimated using a total of 1831 survey responses in China. The psychology-related and policy-related models explained 62.2% and 33.6% of the variance in AV adoption intention, respectively. The results suggest that perceived playfulness (β = 0.524, p < 0.001) and information dissemination (β = 0.348, p < 0.001) are the most important influencing factors of AV adoption intention. In addition, demographic factors (gender, education, income, the number of private cars owned by a family, and types of cities) can moderate the effects of psychological factors and policy measures on user intentions to adopt an AV. These insights can be employed to design more cost-effective policies and strategies for subgroups of the population to maximize the AV adoption intention.
Association between Community Social Capital and Access to Dental Check-Ups among Elementary School Children in Japan
Authors: Kobayashi Marie , Matsuyama Yusuke , Nawa Nobutoshi , Isumi Aya , Doi Satomi , Fujiwara Takeo
Published: December 2022

The association between parental social capital and a child’s access to dental check-ups has been reported, but few studies have focused on dental check-ups. The present study investigated the association between parental social capital and access to dental check-ups among first-grade elementary school children (6–7 years old) in Japan. We analyzed cross-sectional data of first-grade elementary school children (6–7 years old) in Adachi City, Tokyo, Japan. Community social capital (the total score of social trust, cohesion, mutual aid) and child’s dental check-ups (dental check-ups for purposes other than treatment at least once a year) were assessed by questionnaire surveys for parents of the first graders from all 69 elementary schools in 2017 (n = 5260; response rate: 81.6%) and 2019 (n = 5130; response rate: 78.8%). Multilevel Poisson regression analysis, adjusted for children’s age in months, gender, mother’s educational attainment, mother’s employment status, having siblings, living with grandparents, and the density of dental clinics in the school district, was applied. Of the 7936 respondents included in the analysis, 82.7% of children received dental check-ups at least once a year. Individual-level social capital was positively associated with children’s dental check-up utilization (prevalence ratio, PR = 0.935 per one interquartile range, IQR; 95% confidence interval, CI: 0.877, 0.996). Community-level social capital was not significantly associated with children’s dental check-up utilization (PR = 0.934 per one IQR; 95% CI: 0.865, 1.008). Promoting individual-level social capital, but not community-level social capital, may improve dental check-ups among elementary school children in Japan.
Work-From-Home in the New Normal: A Phenomenological Inquiry into Employees’ Mental Health
Authors: Memon Mumtaz Ali , Shaikh Saba , Mirza Muhammad Zeeshan , Obaid Asfia , Muenjohn Nuttawuth , Ting Hiram
Published: December 2022

The COVID-19 pandemic has forced employees to adapt and adjust to the new normal in an unprecedented way. While some employees have been able to move to work-from-home (WFH) relatively easily, many find it challenging. Notwithstanding the magnitude of change, little is known about the determinants of WFH employees’ mental health during COVID-19. This study therefore aims to explore (1) the salient factors that contribute to the mental health issues of WFH employees and (2) strategies to overcome WFH challenges. A qualitative approach using phenomenological inquiry was adopted. Forty-one employees who worked from home in Pakistan were sampled using the purposive and snowball sampling techniques. Data was collected via semi-structured interviews and analyzed using thematic analysis. Overall, employees believe that organizations offer inadequate support in both work-related and non-work-related matters. Five themes were elicited and coded as factors that contribute to mental health issues among WFH employees. Technical issues and system complexities, the absence of flexible working arrangements, distractions, a lack of communication, and inadequate social support were found to obstruct WFH and cause mental distress. Behavioral and cognitive coping strategies were also determined to tackle these mental issues. This study complements the human resource literature by exploring the factors that obstruct WFH and cause mental health issues in the context of the pandemic crisis. As mental well-being is more intricate than administrative arrangements, the study is useful for organizations to develop a feasible mechanism that facilitates the smooth execution of WFH for employees while ensuring their mental health is preserved. Using a phenomenological inquiry, the present study is one of the few to explore the factors that contribute to the mental health of WFH employees in the context of the pandemic crisis. Apart from its contribution to knowledge on human resource management and organizational behavior, it provides useful implications for managers, policymakers, and practitioners to manage WFH employees more effectively.
Do the Lower Body Strength Assessment Tests in the Spanish Navy Really Measure What They Purport to Measure?
Authors: Vila Mª Helena , de Oliveira Iris M. , Burgos-Martos Francisco J. , Martín-Pinadero Angel , Mollinedo-Cardalda Irimia , Cancela-Carral José M.
Published: December 2022

The main objective of this research was to analyse the efficacy of lower body strength assessment tests in the Armed Forces Physical Assessment System. Secondly, it was to determine what relationship exists between the physical evaluation system of the Spanish Armed forces and standardized evaluation protocols (Gold standard). A total of 905 students enrolled in the military/civil bachelor’s degree (813 male and 92 female) participated in this study. The influence of the sex of the participants was studied through the student’s t-test for independent data, and the degree of association between variables was defined by Pearson’s correlation coefficient. The results present moderate correlations (r = 0.67, r = 0.66; p < 0.001) between the vertical jump test used by the Army and the power or elastic force tests commonly used in practice and in research. The results obtained reflect a moderate relationship between the gold standard tests and the tests used by the Army, which suggests that the tests currently used to assess lower body strength should be adapted to more objective measurement tools which would allow a better comparison between samples from different armed forces.
Red Cell Distribution Width as a Predictive Factor of Celiac Disease in Middle and Late Adulthood and Its Potential Utility as Celiac Disease Screening Criterion
Authors: Cabo del Riego Julia María , Núñez-Iglesias María Jesús , Paz Carreira José , Blanco Hortas Andrés , Álvarez Fernández Tamara , Novío Mallón Silvia , Zaera Sofía , Freire-Garabal Núñez Manuel
Published: December 2022

Red cell distribution width (RDW) could be of interest by its potential use in the assessment of celiac disorder (CD). The main objective of this study was to evaluate the case positive rate of CD and the utility of red cell distribution width (RDW) in the CD diagnosis. This prospective study included 9.066 middle adult (≥45 years old) and elderly patients (≥60 years old) from 2012 to 2021. CD diagnosis was performed by CD antibody tests (serology and Human Leucocyte Antigen genotype (HLA)) and biopsy. Gastrointestinal and extra-intestinal manifestations as well as hematological and biochemical parameters were analyzed. CD diagnoses were confirmed in 101 patients (median (IQR) age = 62 (52.3–73); 68.32% women) by serologic tests (100%) and intestinal biopsy (88.12%), showing mainly marked or complete atrophy (76.24%, MARSH 3a–c). Anemia was the most commonly presenting extra-intestinal manifestation (28.57%). Among 8975 individuals without CD, 168 age and sex matched were included. By comparison of CD and no CD individuals, we observed that high >14.3% RDW was exhibited by 58.40% and 35.2% individuals with CD and without CD, respectively. Furthermore, high RDW is associated with CD and grade III atrophy. We suggest that RDW could be used as a CD screening criterion.
Exploration of the Experiences of Persons in the Traumatic Spinal Cord Injury Population in Relation to Chronic Pain Management
Authors: Williams Tammy-Lee , Joseph Conran , Nilsson-Wikmar Lena , Phillips Joliana
Published: December 2022

Chronic pain amongst individuals with traumatic and nontraumatic spinal cord injury (SCI) has high prevalence rates, with severe impact on the activities of daily living, mood, sleep and quality of life. This study aimed to explore the experiences and challenges of chronic pain management amongst the traumatic spinal cord injury (TSCI) population in the Western Cape region of South Africa. A qualitative descriptive approach was chosen for the study, in which 13 individuals living with TSCI were purposively recruited and interviewed telephonically. An inductive thematic analytic approach was used. The results indicate ineffectiveness of standard pain management, with a lack of education regarding pain physiology and pain management strategies as well as unbalanced decision-making between clinician and patient. Thus, patients develop coping strategies to survive with pain. Current pain regimes are suboptimal at best, underpinned by the lack of clarity or a mutually agreed plan to mitigate and eradicate pain. There is a need for chronic pain management beyond pharmacological prescription. Future practices should focus on adopting a holistic, biopsychosocial approach, which includes alternative pain therapy management. In addition, advances in pain management cannot be achieved without adopting a therapeutic alliance between the clinician and patient.
Solving Constrained Mean-Variance Portfolio Optimization Problems Using Spiral Optimization Algorithm
Authors: Febrianti Werry , Sidarto Kuntjoro Adji , Sumarti Novriana
Published: December 2022

Portfolio optimization is an activity for balancing return and risk. In this paper, we used mean-variance (M-V) portfolio models with buy-in threshold and cardinality constraints. This model can be formulated as a mixed integer nonlinear programming (MINLP) problem. To solve this constrained mean-variance portfolio optimization problem, we propose the use of a modified spiral optimization algorithm (SOA). Then, we use Bartholomew-Biggs and Kane’s data to validate our proposed algorithm. The results show that our proposed algorithm can be an efficient tool for solving this portfolio optimization problem.
Hippocampal Deletion of CB1 Receptor Impairs Social Memory and Leads to Age-Related Changes in the Hippocampus of Adult Mice
Authors: Palmisano Michela , Gargano Alessandra , Olabiyi Bolanle Fatimat , Lutz Beat , Bilkei-Gorzo Andras
Published: December 2022

Endocannabinoid system activity declines with age in the hippocampus, along with the density of the cannabinoid receptor type-1 (CB1). This process might contribute to brain ageing, as previous studies showed that the constitutive deletion of the CB1 receptor in mice leads to early onset of memory deficits and histological signs of ageing in the hippocampus including enhanced pro-inflammatory glial activity and reduced neurogenesis. Here we asked whether the CB1 receptor exerts its activity locally, directly influencing hippocampal ageing or indirectly, accelerating systemic ageing. Thus, we deleted the CB1 receptor site-specifically in the hippocampus of 2-month-old CB1flox/flox mice using stereotaxic injections of rAAV-Cre-Venus viruses and assessed their social recognition memory four months later. Mice with hippocampus-specific deletion of the CB1 receptor displayed a memory impairment, similarly as observed in constitutive knockouts at the same age. We next analysed neuroinflammatory changes in the hippocampus, neuronal density and cell proliferation. Site-specific mutant mice had enhanced glial cell activity, up-regulated levels of TNFα in the hippocampus and decreased cell proliferation, specifically in the subgranular zone of the dentate gyrus. Our data indicate that a local activity of the CB1 receptor in the hippocampus is required to maintain neurogenesis and to prevent neuroinflammation and cognitive decline.
Variability in the Response against Teladorsagia circumcincta in Lambs of Two Canarian Sheep Breeds
Authors: Pérez-Hernández Tara , Hernández Julia N. , Machín Cynthia , McNeilly Tom N. , Nisbet Alasdair J. , Matthews Jacqueline B. , Burgess Stewart T. G. , González Jorge F.
Published: December 2022

The increasing resistance to anthelmintics has necessitated the exploration of alternative control strategies of gastrointestinal nematode (GIN) infections. A sustainable option is genetic selection based on differences in susceptibility to GIN infection between and within breeds of sheep. Here, three-month-old Canaria Hair breed (GIN-resistant) and Canaria Sheep breed (GIN-susceptible) showed no significant between-breed differences after trickle infection with Teladorsagia circumcincta, whereas considerable individual variability was found in both breeds. Next, data from lambs of both breeds were used to explore the relationships between parasitological variables and T. circumcincta-specific IgA levels, local immune cell populations, and abomasal lymph node gene expression to understand the possible mechanisms underlying resistance. Mucosal IgA levels as well as numbers of globular leukocytes and MHC-II+ cells were associated with protection. Analysis of lymph node gene expression revealed the associations between lower parasite numbers and cumulative fecal egg counts and several immune pathways, such as leukocyte cell adhesion, activation and differentiation of T cells, in particular CD4+ and IL-4 production. The data obtained here may inform on the relationship between phenotypic resistance variability and protective responses at the humoral, cellular, and transcriptomic levels, thus contributing to identifying immune responses in young lambs that could be used as markers for selection.
The Improvement of Functional State of Brain Mitochondria with Astaxanthin in Rats after Heart Failure
Authors: Baburina Yulia , Krestinin Roman , Fedorov Dmitry , Odinokova Irina , Pershina Ekaterina , Sotnikova Linda , Krestinina Olga
Published: December 2022

The relationship between neurological damage and cardiovascular disease is often observed. This type of damage is both a cause and an effect of cardiovascular disease. Mitochondria are the key organelles of the cell and are primarily subject to oxidative stress. Mitochondrial dysfunctions are involved in the etiology of various diseases. A decrease in the efficiency of the heart muscle can lead to impaired blood flow and decreased oxygen supply to the brain. Astaxanthin (AST), a marine-derived xanthophyll carotenoid, has multiple functions and its effects have been shown in both experimental and clinical studies. We investigated the effects of AST on the functional state of brain mitochondria in rats after heart failure. Isoproterenol (ISO) was used to cause heart failure. In the present study, we found that ISO impaired the functional state of rat brain mitochondria (RBM), while the administration of AST resulted in an improvement in mitochondrial efficiency. The respiratory control index (RCI) in RBM decreased with the use of ISO, while AST administration led to an increase in this parameter. Ca2+ retention capacity (CRC) decreased in RBM isolated from rat brain after ISO injection, and AST enhanced CRC in RBM after heart failure. The study of changes in the content of regulatory proteins such as adenine nucleotide translocase 1 and 2 (ANT1/2), voltage dependent anion channel (VDAC), and cyclophilin D (CyP-D) of mitochondrial permeability transition pore (mPTP) showed that ISO reduced their level, while AST restored the content of these proteins almost to the control value. In general, AST improves the functional state of mitochondria and can be considered as a prophylactic drug in various therapeutic approaches.
Ion and Water Transport in Ion-Exchange Membranes for Power Generation Systems: Guidelines for Modeling
Authors: Mareev Semyon , Gorobchenko Andrey , Ivanov Dimitri , Anokhin Denis , Nikonenko Victor
Published: December 2022

Artificial ion-exchange and other charged membranes, such as biomembranes, are self-organizing nanomaterials built from macromolecules. The interactions of fragments of macromolecules results in phase separation and the formation of ion-conducting channels. The properties conditioned by the structure of charged membranes determine their application in separation processes (water treatment, electrolyte concentration, food industry and others), energy (reverse electrodialysis, fuel cells and others), and chlore-alkali production and others. The purpose of this review is to provide guidelines for modeling the transport of ions and water in charged membranes, as well as to describe the latest advances in this field with a focus on power generation systems. We briefly describe the main structural elements of charged membranes which determine their ion and water transport characteristics. The main governing equations and the most commonly used theories and assumptions are presented and analyzed. The known models are classified and then described based on the information about the equations and the assumptions they are based on. Most attention is paid to the models which have the greatest impact and are most frequently used in the literature. Among them, we focus on recent models developed for proton-exchange membranes used in fuel cells and for membranes applied in reverse electrodialysis.
UVB-Pretreatment-Enhanced Cadmium Absorption and Enrichment in Poplar Plants
Authors: He Fang , Zhao Qian , Shi Yu-Jie , Li Jun-Lin , Wang Ting , Lin Tian-Tian , Zhao Kuang-Ji , Chen Liang-Hua , Mi Jia-Xuan , Yang Han-Bo , Zhang Fan , Wan Xue-Qin
Published: December 2022

The phenomenon of cross adaptation refers to the ability of plants to improve their resistance to other stress after experiencing one type of stress. However, there are limited reports on how ultraviolet radiation B (UVB) pretreatment affects the enrichment, transport, and tolerance of cadmium (Cd) in plants. Since an appropriate UVB pretreatment has been reported to change plant tolerance to stress, we hypothesized that this application could alter plant uptake and tolerance to heavy metals. In this study, a woody plant species, 84K poplar (Populus alba × Populus glandulosa), was pretreated with UVB and then subjected to Cd treatment. The RT-qPCR results indicated that the UVB-treated plants could affect the expression of Cd uptake, transport, and detoxification-related genes in plants, and that the UVB-Pretreatment induced the ability of Cd absorption in plants, which significantly enriched Cd accumulation in several plant organs, especially in the leaves and roots. The above results showed that the UVB-Pretreatment further increased the toxicity of Cd to plants in UVB-Cd group, which was shown as increased leaf malonaldehyde (MDA) and hydrogen peroxide (H2O2) content, as well as downregulated activities of antioxidant enzymes such as Superoxide Dismutase (SOD), Catalase (CAT), and Ascorbate peroxidase (APX). Therefore, poplar plants in the UVB-Cd group presented a decreased photosynthesis and leaf chlorosis. In summary, the UVB treatment improved the Cd accumulation ability of poplar plants, which could provide some guidance for the potential application of forest trees in the phytoremediation of heavy metals in the future.
Atomic Force Microscopy Reveals Complexity Underlying General Secretory System Activity
Authors: Weaver Dylan R. , King Gavin M.
Published: December 2022

The translocation of specific polypeptide chains across membranes is an essential activity for all life forms. The main components of the general secretory (Sec) system of E. coli include integral membrane translocon SecYEG, peripheral ATPase SecA, and SecDF, an ancillary complex that enhances polypeptide secretion by coupling translocation to proton motive force. Atomic force microscopy (AFM), a single-molecule imaging technique, is well suited to unmask complex, asynchronous molecular activities of membrane-associated proteins including those comprising the Sec apparatus. Using AFM, the dynamic structure of membrane-external protein topography of Sec system components can be directly visualized with high spatial-temporal precision. This mini-review is focused on AFM imaging of the Sec system in near-native fluid conditions where activity can be maintained and biochemically verified. Angstrom-scale conformational changes of SecYEG are reported on 100 ms timescales in fluid lipid bilayers. The association of SecA with SecYEG, forming membrane-bound SecYEG/SecA translocases, is directly visualized. Recent work showing topographical aspects of the translocation process that vary with precursor species is also discussed. The data suggests that the Sec system does not employ a single translocation mechanism. We posit that differences in the spatial frequency distribution of hydrophobic content within precursor sequences may be a determining factor in mechanism selection. Precise AFM investigations of active translocases are poised to advance our currently vague understanding of the complicated macromolecular movements underlying protein export across membranes.