bluuebunny/crossref_metadata_2025 huggingface dataset:
Surgical Treatment of Dysphagia Secondary to Anterior Cervical Osteophytes Due to Diffuse Idiopathic Skeletal Hyperostosis
Authors: Choi Ho Yong , Jo Dae Jean
Published: July 2022

Diffuse idiopathic skeletal hyperostosis (DISH) is an ossifying and ankylosing skeletal condition that can be associated with DISH-related dysphagia in the case of cervical involvement. In contrast to most cases of dysphagia, which are managed conservatively, DISH-related dysphagia can be discouraging due to the progressive nature of DISH. We report two cases of DISH-related dysphagia that were treated with the surgical removal of osteophytes via an anterolateral approach. We were able to remove osteophytes using the bottleneck point as an anatomical landmark between the vertebral body and the bony excrescence. Patients’ symptoms improved following osteophyte removal, without recurrence. In cases of DISH-related dysphagia, osteophyte removal using an osteotome could improve dysphagia safely and quickly.
In Situ Observation of Tensile Deformation of Ti-22Al-25Nb Alloy and Characterization of Deformation in α2 Phase
Authors: Li Dong , Zeng Weidong , Zhang Penghui , Ma Haoyuan , Xu Jianwei , Ma Xiong
Published: July 2022

The room temperature tensile deformation of Ti-22Al-25Nb alloy with an equiaxed α2 phase microstructure and the activated slip system of α2 particles were investigated by a combination of in situ tensile tests and electron backscatter diffraction experiments. The results demonstrate that only a few wide and long slip bands occur in the B2 matrix in the initial stage of yielding. With the tensile displacement increases, a large number of slip bands, including multiple- and cross-slip bands, appear in the B2 matrix and the distance between two adjacent slip bands decreases significantly. Meanwhile, the movement of the slip bands is hindered by the α2 particles and the B2 grain boundaries, and the slip bands appear only in a small number of the α2 particles. From the beginning of the tensile process to the final fracture, there are lots of α2 particles without slip bands. The slip bands penetrate the needle-like lamellar O phase without changing the slip direction. Compared with the α2 particles, the hindering effect of needle-like O phases on the motion of the slip bands is quite small. The microcracks nucleated at the α2/B2 phase boundaries or within the α2 particles, and microcracks propagated along the α2/B2 phase boundaries or across the α2 particles. The fracture surface shows the quasi-cleavage feature, which contains a large number of small and shallow dimples on planar facets. The analysis indicates that the plastic deformation of the alloy is mainly contributed by the B2 phase. For room temperature tensile deformation of α2 phase, there are three types of slip systems that can be activated, including the prism  type slip, the basal  type slip and the pyramidal  type slip. The prism  type slip is most likely to be activated, followed by the basal  type slip and finally the pyramidal  type slip. In addition, the critical resolved shear stress (CRSS) for the pyramidal  type slip is the highest among the three types of slip systems. Therefore, the deformation in the α2 phase is mainly contributed by the prism  type slip and the basal  type slip.
Challenges and Latest Developments in Diffusion Bonding of High-Magnesium Aluminium Alloy (Al-5056/Al-5A06) to Stainless Steels
Authors: Shirzadi Amir A. , Zhang Chengcong , Mughal Muhammad Zeeshan , Xia Peiyun
Published: July 2022

The aim of this work was to investigate the challenges associated with bonding Al-Mg alloys and develop a new method for bonding these alloys to steels. During an extensive R&D project, over 80 attempts, using 11 methods, were made to bond Al-6 wt.% Mg alloy (Al-5056/Al-5A06) to two types of stainless steels (heat-resistant 1Cr18Ni9Ti and conventional 316). Wide ranges of temperature (500 °C to 580 °C), pressure (0.5 MPa to 10 MPa) and time (1 min to 2 h) were used when direct diffusion bonding of these alloys. Then, effects of using various interlayers and brazing foils were investigated. The interlayers used in this work were gallium, pure titanium, copper and aluminium foils, aluminium 6061 alloy sheets, aluminium-silicon brazing foils, zinc and zinc alloy foils as well as an active brazing foil (known as Incusil-ABA containing silver, copper, indium and titanium). Several complex and multi-stage processes, using up to 3 different interlayers in the same joint, were also developed and assessed. Examination and assessment of the bonded samples, including failed attempts, paved the way of developing new methods for bonding these dissimilar materials. A number of samples with tensile strengths from 200 MPa to 226 MPa were made by using complex combinations of 2 or 3 interlayers and triple-stage bonding cycles. The highest recorded bond strength was 226 MPa in the as-bonded condition. This value is above the measured yield strength (134 MPa) and about 93% of the measured ultimate strength (243 MPa) of the parent Al-Mg alloy after it was subjected to the same bonding cycle. Since the use of complex processes was not feasible for bonding large components, a simpler and more practical bonding cycle was also developed in the project. Using the simpler process, joints with tensile strengths around 90 MPa could be made. This article also sheds light on the difficulties associated with brazing and soldering aluminium alloys with a high magnesium content.
Impact of Cattle Feeding Strategy on the Beef Metabolome
Authors: Gómez Juan Fernando Morales , Cônsolo Nara Regina Brandão , Antonelo Daniel Silva , Beline Mariane , Gagaoua Mohammed , Higuera-Padilla Angel , Colnago Luiz Alberto , Gerrard David Edwin , Silva Saulo Luz
Published: July 2022

The present study explored changes in the meat metabolome of animals subjected to different finishing systems and growth rates. Thirty-six Angus × Nellore crossbred steers were used in a completely randomized design with four treatments: (1) feedlot system with high average daily gain (ADG; FH); (2) feedlot system with low ADG (FL); (3) pasture system with high ADG (PH); and (4) pasture system with low ADG (PL). After harvest and chilling, Longissimus thoracis (LT) muscle samples were taken for metabolite profile analysis using nuclear magnetic resonance. Spectrum was analyzed using chenomx software, and multi- and mega-variate data analyses were performed. The PLS-DA showed clear separation between FH and PL groups and overlap among treatments with different finishing systems but similar for matching ADG (FL and PH) treatments. Using a VIP cut-off of around 1.0, ATP and fumarate were shown to be greater in meat from PL cattle, while succinate, leucine, AMP, glutamate, carnosine, inosine, methionine, G1P, and choline were greater in meat from FH. Comparing FL and PH treatments, glutamine, carnosine, urea, NAD+, malonate, lactate, isoleucine, and alanine were greater in the meat of PH cattle, while G6P and betaine were elevated in that of FL cattle. Relevant pathways were also identified by differences in growth rate (FH versus PL) and finishing system were also noted. Growth rate caused a clear difference in meat metabolism that was highlighted by energy metabolism and associated pathways, while the feeding system tended to alter protein and lipid metabolism.
Special Issue on Metabolism and Metabolite Markers in Type 2 Diabetes
Authors: Eriksson Jan W.
Published: July 2022

It is well recognized that type 2 diabetes poses a major and increasing public health challenge, and presently, almost 500 million individuals around the globe are diagnosed with this condition [...]
Probabilistic Evaluation of the Multicategory Seasonal Precipitation Re-Forecast
Authors: Xu Yiwen
Published: July 2022

The Meteo-France seasonal forecasting system 7 provides a 7-month forecast range with 25 ensembles. The seasonal precipitation re-forecast (from May to November 1993–2015) was evaluated by the Brier score in terms of accuracy and reliability based on tercile probabilities. Multiple analyses were performed to assess the robustness of the score. These results show that the spatial distribution of the Brier score depends significantly on tercile thresholds, reference data, sampling methods, and ensemble types. Large probabilistic errors over the dry regions on land and the Nino regions in the Pacific can be reduced by adjusting the tercile thresholds. The forecast errors were identified when they were insensitive to different analysis methods. All the analyses detected that the errors increase/decrease with the lead time over the tropical Indian/Pacific Ocean. The intra-seasonal analysis reveals that some of these errors are inherited from monthly forecasts, which may be related to large-scale, short-term variability modes. A new confidence interval calculation was formulated for the “uncertain” case in the reference data. The confidence interval at a 95% level for the mean Brier score over the entire tropical region was quantified. The best estimations are ~6% the mean Brier score for both the above and below-normal terciles.
Simulation Analysis and Performance Test of a Compressible Piezoelectric Pump Fluid Cavity
Authors: Liu Xia , Wang Tongyu , Wang Hu , Hou Jun , Liu Jinlong , Lin Jiaying , Li Shenfang , Wang Zhicong , Tian Xiaochao , Yang Zhigang
Published: July 2022

The large load loss of piezoelectricity pumps leads to fluid energy in the fluid chamber during fluid transportation. In this paper, the output performance of a piezoelectricity pump is improved by changing the structure parameters of the fluid chamber to reduce the fluid load. The mechanism of fluid flow energy loss in the body cavity of hydraulic pumps is simulated and analyzed, and the influence of the dimensions of the inlet and outlet valves and the height of the cavity on fluid energy loss is obtained. The flow rate and pressure of inlet and outlet valves with different cavity heights and different driving frequencies are obtained. The results show that the flow rate and output pressure of the hydraulic pump are optimized when the cavity height is 3 mm, and the inlet and outlet valve diameters are 2.5 mm.
Sequence-Specific Electrochemical Genosensor for Rapid Detection of blaOXA-51-like Gene in Acinetobacter baumannii
Authors: Kanapathy Swarnaletchumi , Obande Godwin Attah , Chuah Candy , Shueb Rafidah Hanim , Yean Chan Yean , Banga Singh Kirnpal Kaur
Published: July 2022

Acinetobacter baumannii (A. baumannii) are phenotypically indistinguishable from the Acinetobacter calcoaceticus–A. baumannii (ACB) complex members using routine laboratory methods. Early diagnosis plays an important role in controlling A. baumannii infections and this could be assisted by the development of a rapid, yet sensitive diagnostic test. In this study, we developed an enzyme-based electrochemical genosensor for asymmetric PCR (aPCR) amplicon detection of the blaOXA-51-like gene in A. baumannii. A. baumanniiblaOXA-51-like gene PCR primers were designed, having the reverse primer modified at the 5′ end with FAM. A blaOXA-51-like gene sequence-specific biotin labelled capture probe was designed and immobilized using a synthetic oligomer (FAM-labelled) deposited on the working electrode of a streptavidin-modified, screen-printed carbon electrode (SPCE). The zot gene was used as an internal control with biotin and FAM labelled as forward and reverse primers, respectively. The blaOXA-51-like gene was amplified using asymmetric PCR (aPCR) to generate single-stranded amplicons that were detected using the designed SPCE. The amperometric current response was detected with a peroxidase-conjugated, anti-fluorescein antibody. The assay was tested using reference and clinical A. baumannii strains and other nosocomial bacteria. The analytical sensitivity of the assay at the genomic level and bacterial cell level was 0.5 pg/mL (1.443 µA) and 103 CFU/mL, respectively. The assay was 100% specific and sensitive for A. baumannii. Based on accelerated stability performance, the developed genosensor was stable for 1.6 years when stored at 4 °C and up to 28 days at >25 °C. The developed electrochemical genosensor is specific and sensitive and could be useful for rapid, accurate diagnosis of A. baumannii infections even in temperate regions.
Adsorption of PAHs and PCDD/Fs in Microplastics: A Review
Authors: Conesa Juan A.
Published: July 2022

The presence of microplastics (MPs) in the environment, and the effects that the ingestion of these materials can have on organisms, can be aggravated by the adsorption of harmful substances on the surface or inside the MPs. Of special relevance are the studies that have been carried out on the adsorption and transport of polycyclic aromatic hydrocarbons (PAHs) as well as persistent organic pollutants (POPs) such as dioxins and furans (PCDD/Fs). This review will delve into the research carried out to date regarding the adsorption by conventional and biodegradable MPs of dangerous organic compounds such as those mentioned. In general, the presence of MPs is considered a vector for the entry of these contaminants into living beings, since their capacity to adsorb contaminants is very high and they are ingested by different organisms that introduce these contaminants into the trophic chain.
Petrogenesis and Tectonic Implications of the Triassic Granitoids in the Ela Mountain Area of the East Kunlun Orogenic Belt
Authors: Pan Zhongcui , Sun Fengyue , Cong Zhichao , Tian Nan , Xin Wei , Wang Li , Zhang Yajing , Wu Dongqian
Published: July 2022

The East Kunlun Orogenic Belt is located in the western part of the Central Orogenic Belt of China, with a large number of Triassic igneous rocks parallel to the Paleo-Tethys ophiolite belt, which provides a large amount of geological information for the tectonic evolution of the Paleo-Tethys Ocean. The granitoids studied in this paper are located in the Ela Mountain area in the eastern part of the East Kunlun Orogenic Belt. Zircon U-Pb dating results show that these different types of granitoids were crystallized in the Triassic. The 247.5 Ma porphyritic granites from Zairiri (ZRR) displayed calc-alkaline I-type granite affinities, with the zircon εHf(t) values being mainly positive (−0.5 to + 3.8, TDM2 of 1309–1031 Ma), indicating that they are derived from the partial melting of the juvenile crust and mixed with ancient crustal components. The 236.8 Ma Henqionggou (HQG) granodiorites and 237.5 Ma Daheba (DHB) granodiorites are high-K calc-alkaline I-type granite, and both have mafic microgranular enclaves (MMEs), showing higher and more varied Mg# (39.73–62.73), combined with their negative Hf isotopes (εHf(t) = −2.6 to −1.6, TDM2 = 1430–1369 Ma), suggesting that their primary magmas were the products of partial melting of the Mesoproterozoic lower crust that mixed with mantle-derived rocks. The 236.4 Ma DHB porphyritic diorites showed characteristics of high-K calc-alkaline I-type granitoids, with moderate SiO2 contents, medium Mg# values (40.41–40.65), with the Hf isotopes (εHf(t) = −2.9 to −0.5; TDM2 = 1451–1298 Ma) indistinguishably relative to contemporaneous host granodiorites and MMEs. The petrographic and geochemical characteristics indicate that the porphyritic diorites are the product of well-mixed magma derived from the Mesoproterozoic lower crust and lithospheric mantle. Based on the results of this paper and previous data, the chronology framework of Late Permian–Triassic magmatic rocks in the eastern part of the East Kunlun Orogenic Belt was constructed, and the magmatic activities in this area were divided into three peak periods, with each peak representing an extensional event in a particular tectonic setting, for example, P1 (slab roll-back in subduction period; 254–246 Ma), P2 (slab break-off in transition period of subduction and collision; 244–232 Ma), P3 (delamination after collision; 230–218 Ma).
Investigation of the 176Yb Interference Correction during Determination of the 176Hf/177Hf Ratio by Laser Ablation and Solution Analysis on the Neoma MC-ICP-MS
Authors: Zirakparvar Nasser A. , Manard Benjamin T. , Hexel Cole R. , Dunlap Daniel R.
Published: July 2022

We utilized the Neoma™, a recently released MC-ICP-MS platform offered by ThermoFisher Scientific, to assess the behavior of the Lu-Yb-Hf system during laser ablation analyses of various zircon standards as well as solution-based analyses of the JMC-475 Hf standard doped with varying quantities of Yb and Lu. The primary goal of this work was to characterize the behavior of the Yb interference correction on the Neoma™ platform since this is one of the biggest issues in the Hf isotope analysis community and because the Neoma™ platform will supplant the Neptune™ series instrument. During laser ablation analysis, we found that the overall data quality scales proportionally with the total Hf signal intensity, with higher signal analyses producing extremely accurate (within 1 εHf unit) and precise (sub εHf unit within-run standard errors) data. At low Yb signals (<0.1 V 173Yb), we were not able to produce an accurate internal Yb mass bias factor. However, utilizing an empirical approach allows for the application of session-specific relationships between the Yb and Hf mass bias factors, determined by analysis of standards of varying Yb content, to produce accurate εHf values from zircons with higher Yb/Hf ratios even where the total Hf signal intensity is relatively low. Similar behavior was observed in the solution analyses. Lastly, while the behavior of the Yb interference correction on the Neoma™ platform appears comparable to the Neptune™ series MC-ICP-MS, further work will help refine the understanding of the controls on mass bias behavior, oxide formation, session-to-session stability, etc.
Environmental Fate of Organic Sunscreens during Water Disinfection Processes: The Formation of Degradation By-Products and Their Toxicological Profiles
Authors: Medici Antonio , Luongo Giovanni , Di Fabio Giovanni , Zarrelli Armando
Published: July 2022

The development of any commercial product should also be aimed at reducing the risk associated with it, according to the safe-by-design concept; that is, risk assessment should always be at the center of the design, and the impact on human and environmental health should be assessed and eliminated during the product development phase and not afterwards. Unfortunately, even today, most operators in any production sector implement the philosophy of “risk management” or rather of managing the problem when it occurs, using spot interventions instead of changing the approach. This argument is also valid in the production of solar filters, which have reached a satisfactory degree of efficiency in the face of a substantial underestimation of the risks associated with their possible environmental fate. In fact, solar filters have been found in bathing waters and their environmental fate may depend on various factors such as the pH of the water, the presence of organic material, metal ions and light, and, above all, the chemical agents used in the disinfection of the water itself. Thus, during disinfection processes, the generation of dozens of products with a lower molecular weight and generally of an aromatic nature has been tested, where some of them did not receive an exact structural definition and a precise evaluation of their precise toxicological profile. Therefore, it is interesting to draw a complete picture of organic sunscreens and of the byproducts obtained under different conditions and their related ecotoxicological profile.
Development and Optimization of Ciprofloxacin HCl-Loaded Chitosan Nanoparticles Using Box–Behnken Experimental Design
Authors: Soliman Noha M. , Shakeel Faiyaz , Haq Nazrul , Alanazi Fars K. , Alshehri Sultan , Bayomi Mohsen , Alenazi Ahmed S. M. , Alsarra Ibrahim A.
Published: July 2022

Various chitosan (CS)-based nanoparticles (CS-NPs) of ciprofloxacin hydrochloride (CHCl) have been investigated for therapeutic delivery and to enhance antimicrobial efficacy. However, the Box–Behnken design (BBD)-supported statistical optimization of NPs of CHCl has not been performed in the literature. As a result, the goal of this study was to look into the key interactions and quadratic impacts of formulation variables on the performance of CHCl-CS-NPs in a systematic way. To optimize CHCl-loaded CS-NPs generated by the ionic gelation process, the response surface methodology (RSM) was used. The BBD was used with three factors on three levels and three replicas at the central point. Tripolyphosphate, CS concentrations, and ultrasonication energy were chosen as independent variables after preliminary screening. Particle size (PS), polydispersity index (PDI), zeta potential (ZP), encapsulation efficiency (EE), and in vitro release were the dependent factors (responses). Prepared NPs were found in the PS range of 198–304 nm with a ZP of 27–42 mV. EE and drug release were in the range of 23–45% and 36–61%, respectively. All of the responses were optimized at the same time using a desirability function based on Design Expert® modeling and a desirability factor of 95%. The minimum inhibitory concentration (MIC) of the improved formula against two bacterial strains, Pseudomonas aeruginosa and Staphylococcus aureus, was determined. The MIC of the optimized NPs was found to be decreased 4-fold compared with pure CHCl. The predicted and observed values for the optimized formulation were nearly identical. The BBD aided in a better understanding of the intrinsic relationship between formulation variables and responses, as well as the optimization of CHCl-loaded CS-NPs in a time- and labor-efficient manner.
Effects of Dietary α-Linolenic Acid Treatment and the Efficiency of Its Conversion to Eicosapentaenoic and Docosahexaenoic Acids in Obesity and Related Diseases
Authors: Takic Marija , Pokimica Biljana , Petrovic-Oggiano Gordana , Popovic Tamara
Published: July 2022

The essential fatty acid alpha-linolenic acid (ALA) is present in high amounts in oils such as flaxseed, soy, hemp, rapeseed, chia, and perilla, while stearidonic acid is abundant in echium oil. ALA is metabolized to eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) by desaturases and elongases in humans. The conversion of ALA to EPA and DHA is limited, and these long-chain n−3 polyunsaturated fatty acids (PUFAs) are mainly provided from dietary sources (fish and seafood). This review provides an overview of studies that explored the effects of dietary supplementation with ALA in obesity and related diseases. The obesity-associated changes of desaturase and elongase activities are summarized, as they could influence the metabolic conversion of ALA. Generally, supplementation with ALA or ALA-rich oils leads to an increase in EPA levels and has no effect on DHA or omega-3 index. According to the literature data, stearidonic acid could enhance conversion of ALA to long-chain n−3 PUFA in obesity. Recent studies confirm that EPA and DHA intake should be considered as a primary dietary treatment strategy for improving the omega-3 index in obesity and related diseases.
Determination of Osimertinib, Aumolertinib, and Furmonertinib in Human Plasma for Therapeutic Drug Monitoring by UPLC-MS/MS
Authors: Li Ying , Meng Lu , Ma Yinling , Li Yajing , Xing Xiaoqing , Guo Caihui , Dong Zhanjun
Published: July 2022

The third-generation epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs), osimertinib, aumolertinib, and furmonertinib represent a new treatment option for patients with EGFR p.Thr790 Met (T790 M)-mutated non-small cell lung cancer (NSCLC). Currently, there are no studies reporting the simultaneous quantification of these three drugs. A simple ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous quantitative determination of osimertinib, aumolertinib, and furmonertinib concentrations in human plasma, and it was applied for therapeutic drug monitoring (TDM). Plasma samples were processed using the protein precipitation method (acetonitrile). A positive ion monitoring mode was used for detecting analytes. D3-Sorafenib was utilized as the internal standard (IS), and the mobile phases were acetonitrile (containing 0.1% formic acid) and water with gradient elution on an XSelect HSS XP column (2.1 mm × 100.0 mm, 2.5 µm, Waters, Milford, MA, USA) at a flow rate of 0.5 mL·min−1. The method’s selectivity, precision (coefficient of variation of intra-day and inter-day ≤ 6.1%), accuracy (95.8–105.2%), matrix effect (92.3–106.0%), extraction recovery, and stability results were acceptable according to the guidelines. The linear ranges were 5–500 ng·mL−1, 2–500 ng·mL−1, and 0.5–200 ng·mL−1 for osimertinib, aumolertinib, and furmonertinib, respectively. The results show that the method was sensitive, reliable, and simple and that it could be successfully applied to simultaneously determine the osimertinib, aumolertinib, and furmonertinib blood concentrations in patients. These findings support using the method for TDM, potentially reducing the incidence of dosing blindness and adverse effects due to empirical dosing and inter-patient differences.
A Comparative Study of Methods for the Visualization of Probability Distributions of Geographical Data
Authors: Srabanti Sanjana , Veiga Carolina , Silva Edcley , Lage Marcos , Ferreira Nivan , Miranda Fabio
Published: July 2022

Probability distributions are omnipresent in data analysis. They are often used to model the natural uncertainty present in real phenomena or to describe the properties of a data set. Designing efficient visual metaphors to convey probability distributions is, however, a difficult problem. This fact is especially true for geographical data, where conveying the spatial context constrains the design space. While many different alternatives have been proposed to solve this problem, they focus on representing data variability. However, they are not designed to support spatial analytical tasks involving probability quantification. The present work aims to adapt recent non-spatial approaches to the geographical context, in order to support probability quantification tasks. We also present a user study that compares the efficiency of these approaches in terms of both accuracy and usability.
Sustainable Green Synthesis of Yttrium Oxide (Y2O3) Nanoparticles Using Lantana camara Leaf Extracts: Physicochemical Characterization, Photocatalytic Degradation, Antibacterial, and Anticancer Potency
Authors: Govindasamy Rajakumar , Govindarasu Mydhili , Alharthi Salman S. , Mani Preeyanghaa , Bernaurdshaw Neppolian , Gomathi Thandapani , Ansari Mohammad Azam , Alomary Mohammad N. , Atwah Banan , Malik M. Shaheer , Rajeswari V. Devi , Rekha Kaliaperumal , Ahmed Saleh A. , Thiruvengadam Muthu
Published: July 2022

Due to their appropriate physicochemical properties, nanoparticles are used in nanomedicine to develop drug delivery systems for anticancer therapy. In biomedical applications, metal oxide nanoparticles are used as powerful and flexible multipurpose agents. This work described a green synthesis of Y2O3 nanoparticles (NPs) using the sol-gel technique with the use of aqueous leaf extracts of Lantana camara L (LC). These nanoparticles were characterized with the aid of different methods, including UV, X-ray diffraction (XRD), Fourier transformed infrared spectroscopy (FTIR), transmitted electron microscopy (TEM), and photocatalytic degradation. Y2O3 nanoparticles showed excellent antibacterial activity against Gram-positive Bacillus subtilis and Gram-negative Escherichia coli with a 10 to 15 mm inhibitory zone. Green Y2O3 NPs were released with a 4 h lag time and 80% sustained release rate, indicating that they could be used in drug delivery. In addition, the bioavailability of green Y2O3 NPs was investigated using cell viability in cervical cancer cell lines. These green-synthesized Y2O3 NPs demonstrated photocatalytic degradation, antibacterial, and anticancer properties.
Review on Perovskite Semiconductor Field–Effect Transistors and Their Applications
Authors: Abiram Gnanasampanthan , Thanihaichelvan Murugathas , Ravirajan Punniamoorthy , Velauthapillai Dhayalan
Published: July 2022

Perovskite materials are considered as the most alluring successor to the conventional semiconductor materials to fabricate solar cells, light emitting diodes and electronic displays. However, the use of the perovskite semiconductors as a channel material in field effect transistors (FET) are much lower than expected due to the poor performance of the devices. Despite low attention, the perovskite FETs are used in widespread applications on account of their unique opto-electrical properties. This review focuses on the previous works on perovskite FETs which are summarized into tables based on their structures and electrical properties. Further, this review focuses on the applications of perovskite FETs in photodetectors, phototransistors, light emitting FETs and memory devices. Moreover, this review highlights the challenges faced by the perovskite FETs to meet the current standards along with the future directions of these FETs. Overall, the review summarizes all the available information on existing perovskite FET works and their applications reported so far.
Impact of Dietitian-Led Nutrition Therapy of Food Order on 5-Year Glycemic Control in Outpatients with Type 2 Diabetes at Primary Care Clinic: Retrospective Cohort Study
Authors: Nitta Ayasa , Imai Saeko , Kajiayama Shizuo , Matsuda Mikuko , Miyawaki Takashi , Matsumoto Shinya , Kajiyama Shintaro , Hashimoto Yoshitaka , Ozasa Neiko , Fukui Michiaki
Published: July 2022

The aim of this retrospective cohort study was to evaluate the effect of 5-year follow-up of dietitian-led medical nutrition therapy (eating vegetables before carbohydrates) on glycemic control in outpatients with type 2 diabetes (T2DM) at a primary care clinic. A total of 138 patients with dietitian-led medical nutrition therapy (intervention group) and 104 patients without dietitian-led nutrition therapy (control group) were compared for glycemic control, serum lipid, blood pressure, and diabetic complications for 5 years. Each patient in the intervention group received dietary education focused on food order (eating vegetables before carbohydrates) by dietitians. A significant improvement in HbA1c after 5 years in the intervention group [8.5 ± 1.7% (69 mmol/mol) to 7.6 ± 1.1% (59 mmol/mol), p < 0.001] was observed, whereas no change was observed in the control group [7.9 ± 1.2% (62 mmol/mol) to 8.0 ± 1.2% (63 mmol/mol)]. Dietary intake of protein, fat, carbohydrates, cholesterol, and salt in the intervention group demonstrated significant reduction, while the intake of dietary fiber significantly increased after the dietary education. Simple dietary education of ‘eating vegetables before carbohydrates’ presented by dietitians achieved good glycemic control after a 5-year period in outpatients with T2DM at primary care clinic.
How the “Are We Drinking Ourselves Sick?” Communication Campaign Built Support for Policy Action on Sugary Drinks in Jamaica
Authors: Christian Donnelle , Maharjan Meena , Kotov Alexey , Cotter Trish , Mullin Sandra , Nurse Vonetta , McGaw Barbara , Chen Deborah , Puri Pallavi , Wang Shuo , Negi Nalin Singh , Murukutla Nandita
Published: July 2022

Background: This study assesses the effectiveness of a campaign “Are We Drinking Ourselves Sick?” that ran nationally in Jamaica in four phases from 2017 to 2019 to increase knowledge about the harms of sugary drinks, shift attitudes, and build support for policy actions to address sugary drink consumption, including a tax and a ban in schools. Methods: Campaign impact was measured in representative cross-sectional household surveys of adults ages 18 to 55. A baseline survey was conducted before the launch of the campaign (n = 1430). Evaluation surveys were conducted mid-campaign (n = 1571) and post-campaign (n = 1500). Campaign impact was assessed by comparing changes across survey periods on key knowledge, attitudinal and policy support outcome indicators. The independent association between campaign awareness and outcomes was analyzed using logistic regression analyses. Results: The campaign was recalled by more than 80% of respondents and was well-received with 90% or more respondents describing it as believable and relevant. There was a decline in knowledge on the harms of sugary drinks from the baseline to post-campaign period, notably on risks of diabetes (adjusted odds ratio or AOR = 0.62, p < 0.001), overweight and obesity (AOR = 0.58, p < 0.001), and heart disease (AOR = 0.79, p < 0.003). However, post-campaign awareness was independently associated in logistic regression analysis with improved knowledge of the harms of sugary drinks, including risks of diabetes (AOR = 1.45, p = 0.019), overweight or obesity (AOR = 1.65, p = 0.001), and heart disease (AOR = 1.44, p = 0.011). Support for government action remained high across survey waves (≥90%), and campaign awareness was independently associated with increased policy support for sugary drinks taxes (Mid-campaign: AOR = 1.43, p = 0.019; post-campaign: AOR = 1.46, p = 0.01) and restrictions on sugary drinks in schools (AOR = 1.55, p = 0.01). Conclusion: This study demonstrates the role that media campaigns can play in maintaining knowledge and concern about the health harms of sugary drinks and increasing support for policy passage.
Assessment of the Effect of Selenium Supplementation on Production of Selected Cytokines in Women with Hashimoto’s Thyroiditis
Authors: Kryczyk-Kozioł Jadwiga , Prochownik Ewelina , Błażewska-Gruszczyk Anna , Słowiaczek Marian , Sun Qian , Schomburg Lutz , Ochab Ewa , Bartyzel Mirosław , Zagrodzki Paweł
Published: July 2022

The impact of selenium on the course of Hashimoto’s thyroiditis (HT) was mainly assessed by monitoring the titer of antithyroid autoantibodies in most of the studies conducted hitherto. On the other hand, the imbalance in activity of T cells such as Th1, Th2, Th17, and Treg may be relevant in the pathogenesis of this disease. Hence, the assessment of changes in the secretion of cytokines by these cells during selenium supplementation in patients with HT seems to be an important issue and was the main goal of this study. A further aim was to search for correlations among these cytokines, as well as markers of thyroid function, selenium/iodine status in the body, and other biochemical parameters. The group of 29 women with newly diagnosed Hashimoto’s thyroiditis was supplemented with selenium in a dose of 100 µg/day for 6 months. Immunological parameters: interferon γ, tumor necrosis factor α, chemokine CXCL10, interleukin 4, interleukin 1β, interleukin 17, transforming growth factor β, and C-reactive protein, as well as selenium status parameters were determined in serum twice, i.e., before and after supplementation. Selenium supplementation was associated with a change in the production of two cytokines: interferon γ and interleukin 1β, for which a decrease and an increase in concentration were observed, respectively. The partial least squares (PLS) model revealed the presence of many relevant correlations among analyzed parameters. The stage of HT development, degree of thyroid dysfunction, and selenium supplementation of diet are interdependent factors which shape the profile of some cytokines secreted by cells participating in the autoimmunity process.
Fatty Acids: A Safe Tool for Improving Neurodevelopmental Alterations in Down Syndrome?
Authors: Martínez-Cué Carmen , Bartesaghi Renata
Published: July 2022

The triplication of chromosome 21 causes Down syndrome (DS), a genetic disorder that is characterized by intellectual disability (ID). The causes of ID start in utero, leading to impairments in neurogenesis, and continue into infancy, leading to impairments in dendritogenesis, spinogenesis, and connectivity. These defects are associated with alterations in mitochondrial and metabolic functions and precocious aging, leading to the early development of Alzheimer’s disease. Intense efforts are currently underway, taking advantage of DS mouse models to discover pharmacotherapies for the neurodevelopmental and cognitive deficits of DS. Many treatments that proved effective in mouse models may raise safety concerns over human use, especially at early life stages. Accumulating evidence shows that fatty acids, which are nutrients present in normal diets, exert numerous positive effects on the brain. Here, we review (i) the knowledge obtained from animal models regarding the effects of fatty acids on the brain, by focusing on alterations that are particularly prominent in DS, and (ii) the progress recently made in a DS mouse model, suggesting that fatty acids may indeed represent a useful treatment for DS. This scenario should prompt the scientific community to further explore the potential benefit of fatty acids for people with DS.
Telehealth Adoption in an Outpatient Oncology Ward: A Best Practice Implementation Project
Authors: Ventura Filipa , Domingues Helena , Almeida Gisela , Cardoso Daniela , Rodrigues Rogério , Moreira Isabel , Pires Mariana , Gomes Inês , Silva Rosa , Oliveira Cláudia , Cardoso Ana Filipa , Ribeiro Liliana , Costeira Cristina
Published: July 2022

Telehealth is increasingly taking place to support the transition of care and self-management of people living with cancer in outpatient oncology settings. Despite its recognised value, the scientific evidence points to disparities with regard to implementation of telehealth that might compromise the equity of access. Following the Joanna Briggs Institute (JBI) implementation approach, this project aims to promote the implementation of best practice recommendations for telehealth adoption in an outpatient oncology setting. Assisted by the Practical Application of Clinical Evidence System (PACES), the implementation process comprises three phases of (i) a baseline audit, (ii) feedback to the healthcare team and establishment of implementation strategies with the Getting Research into Practice (GRiP) tool, and (iii) a follow-up audit. The project is expected to allow the identification of barriers and facilitators for the implementation of telehealth in outpatient oncology and develop a strategy plan for its adoption, with the involvement of end-users and stakeholders. The successful adoption of telehealth according to the best available evidence will likely enhance equity of access to healthcare and quality of care at a distance.
The Efficiency of Commercial Immunodiagnostic Assays for the Field Detection of Schistosoma japonicum Human Infections: A Meta-Analysis
Authors: Mei Zhongqiu , Lv Shan , Tian Liguang , Wang Wei , Jia Tiewu
Published: July 2022

Although great strides have been achieved, schistosomiasis japonica remains a major public health concern in China. Immunodiagnostics have been widely accepted as the first choice in large-scale screening of Schistosoma japonicum human infections, and indirect hemagglutination test (IHA), enzyme-linked immunosorbent assay (ELISA), and dipstick dye immunoassay (DDIA) are currently the three most common immunological tests for the diagnosis of S. japonicum human infections in China. This meta-analysis aimed to comprehensively assess the performance of IHA, ELISA, and DDIA for the field diagnosis of S. japonicum human infections. A total of 37 eligible publications were enrolled in the final analysis, including 29 Chinese publications and 8 English publications. No significant heterogeneities were detected among the studies reporting ELISA (I2 = 88%, p < 0.05), IHA (I2 = 95%, p < 0.05), or DDIA (I2 = 84%, p < 0.05). DDIA showed the highest pooled sensitivity (90.8%, 95% CI: 84.6% to 94.7%) and IHA presented the highest pooled specificity for detection of S. japonicum human infections (71.6%, 95% CI: 65.9% to 76.7%). Summary receiver operating characteristic (SROC) curve analysis showed that IHA exhibited the highest area under the SROC curve (AUC) (0.88, 95% CI: 0.85 to 0.9), and ELISA presented the lowest AUC (0.85, 95% CI: 0.82 to 0.88). Deeks’ funnel plots indicated no publication bias. IHA presented the highest sensitivity in medium-endemicity regions and the highest specificity for diagnosis of S. japonicum human infections in low-endemicity regions, and ELISA showed the highest diagnostic sensitivity in high-endemicity regions and the highest specificity in medium-endemicity regions, while DDIA exhibited the highest diagnostic sensitivity in high-endemicity regions and the highest specificity in low-endemicity regions. IHA and DDIA presented a higher efficiency for the diagnosis of S. japonicum human infections in marshland and lake regions than in hilly and mountainous regions, while ELISA showed a comparable diagnostic sensitivity between in marshland and lake regions and hilly and mountainous regions (88.3% vs. 88.6%), and a higher specificity in marshland and lake regions than in hilly and mountainous regions (60% vs. 48%). Our meta-analysis demonstrates a comparable diagnostic accuracy of IHA, ELISA, and DDIA for S. japonicum human infections, and the diagnostic sensitivity and specificity of IHA, ELISA, and DDIA vary in types and infection prevalence of endemic regions. DDIA combined with IHA is recommended as a tool for screening chemotherapy targets and seroepidemiological surveys during the stage moving towards schistosomiasis elimination in China. Further studies to examine the effectiveness of combinations of two or three immunological tests for diagnosis of S. japonicum human infections are warranted.
Oral Preparation of Hyaluronic Acid, Chondroitin Sulfate, Curcumin, and Quercetin (Ialuril® Soft Gels) for the Prevention of LUTS after Intravesical Chemotherapy
Authors: Manfredi Celeste , Spirito Lorenzo , Calace Francesco Paolo , Balsamo Raffaele , Terribile Marco , Stizzo Marco , Romano Lorenzo , Napolitano Luigi , Califano Gianluigi , Cirillo Luigi , Fusco Giovanni Maria , Rosati Claudia , Quattrone Carmelo , Sciorio Carmine , Creta Massimiliano , Longo Nicola , De Sio Marco , Arcaniolo Davide
Published: July 2022

Intravesical chemotherapy may cause chemical cystitis and related lower urinary tract symptoms (LUTS). The aims of this study were to evaluate the efficacy and safety of an oral preparation of hyaluronic acid (HA), chondroitin sulfate (CS), curcumin, and quercetin (Ialuril® Soft Gels) to reduce the severity of LUTS in patients with a history of bladder cancer (BCa) undergoing intravesical chemotherapy. We designed a monocentric, randomized, double-blind, placebo-controlled pilot trial. Patients referred to our institute between November 2016 and March 2018 were enrolled. All subjects had non-muscle-invasive BCa and received intravesical chemotherapy with mitomycin C (MMC). Patients were randomized 1:1 in two groups (intervention vs. control). All subjects underwent oral administration (Ialuril® Soft Gels or placebo) starting one week before the first weekly instillation and ending 30 days after the last one, subsequently starting one week before each monthly instillation and ending 14 days after it. International prostate symptom score (IPSS) and 0-100 visual analogue scale (VAS) were used to assess the efficacy of the treatment. Adverse events were also described. Patients were evaluated at baseline and after 1, 4, 7, and 13 months of intravesical chemotherapy. A total of 34 patients were enrolled. The median IPSS score was significantly lower in the intervention group compared to the control group at 4 (13 vs. 17 points; p = 0.038), 7 (10 vs. 18 points; p < 0.001), and 13 (10 vs. 17 points; p = 0.002) months. The median VAS score was significantly lower in the intervention group compared to the control group at 7 (22 vs. 37 points; p = 0.021) and 13 (20 vs. 35 points; p = 0.024) months. No AE specifically related to supplement or placebo was recorded. Oral formulation of HA, CS, quercetin, and curcumin could be an effective and safe supportive therapy against chemical cystitis in patients receiving intravesical chemotherapy for BCa.
Synthesis and Biological Evaluation of Imadazo[1,2-a]pyrazines as Anticancer and Antiviral Agents through Inhibition of CDK9 and Human Coronavirus
Authors: Alsfouk Aisha A. , Alshibl Hanan M. , Alsfouk Bshra A. , Altwaijry Najla A. , Al-Abdullah Ebtehal S.
Published: July 2022

In this work, novel imadazo[1,2-a]pyrazine derivatives were synthesized and evaluated as CDK9 inhibitors. The results of CDK9 assay showed that the derivatives with pyridin-4-yl in position 2 and benzyl in position 3 of imadazo[1,2-a]pyrazine 3c displayed the most potent CDK9 inhibitory activity with IC50 of 0.16 µM. The anti-proliferative effect of the new compounds was examined against breast cancer (MCF7), colorectal cancer (HCT116), and chronic myelogenous leukaemia (K652) cell lines. The data of MTT assay showed that the cytotoxic effect of the inhibitors is correlated to their inhibitory activity against CDK9. Compound 3c exhibited the most potent cytotoxicity effect with average IC50s of three cell lines of 6.66 µM. The drug likeness properties of 3c were predicated in silico and demonstrated that 3c have reasonable physiochemical and pharmacokinetic properties. Selected derivatives were assessed in antiviral assay against human coronavirus 229E. The results of this assay showed that the derivative with pyridin-4-yl in position 2 and cyclohexyl in position 3 of imadazo[1,2-a]pyrazine 3b exhibited the most potent anti-coronaviral activity with IC50 of 56.96 µM and selectivity index of 7.14. The target predication result revealed that 3b showed high affinity to protease enzyme. Docking studies of 3b with COVID-19 main protease was conducted and showed good binding affinity, which confirmed the in vitro assay data.
Immune Checkpoint Inhibitor-Mediated Cancer Theranostics with Radiolabeled Anti-Granzyme B Peptide
Authors: de Aguiar Ferreira Carolina , Heidari Pedram , Ataeinia Bahar , Sinevici Nicoleta , Granito Alyssa , Kumar Hritik Mahajan , Wehrenberg-Klee Eric , Mahmood Umar
Published: July 2022

Although immune checkpoint inhibitors (ICI) have revolutionized cancer management, patient response can be heterogeneous, and the development of ICI resistance is increasingly reported. Novel treatment strategies are necessary not only to expand the use of ICI to previously unresponsive tumor types but also to overcome resistance. Targeted radionuclide therapy may synergize well with ICIs since it can promote a pro-inflammatory tumor microenvironment. We investigated the use of a granzyme B targeted peptide (GZP) as a cancer theranostic agent, radiolabeled with 68Ga (68Ga-GZP) as a PET imaging agent and radiolabeled with 90Y (90Y-GZP) as a targeted radionuclide therapy agent for combinational therapy with ICI in murine models of colon cancer. Our results demonstrate that GZP increasingly accumulates in tumor tissue after ICI and that the combination of ICI with 90Y-GZP promotes a dose-dependent response, achieving curative response in some settings and increased overall survival.