bluuebunny/crossref_metadata_2025 huggingface dataset:
Deciphering Transcriptomic Variations in Hematopoietic Lineages: HSCs, EBs, and MKs
Authors: Dahariya Swati , Enright Anton , Kumar Santosh , Gutti Ravi Kumar
Published: September 2024

In the realm of hematopoiesis, hematopoietic stem cells (HSCs) serve as pivotal entities responsible for generating various blood cell types, initiating both the myeloid and lymphoid branches within the hematopoietic lineage. This intricate process is marked by genetic variations that underscore the crucial role of genes in regulating cellular functions and interactions. Recognizing the significance of genetic factors in this context, this article delves into a genetic perspective, aiming to unravel the biological factors that govern the transition from one cell’s fate to another within the hematopoietic system. To gain deeper insights into the genetic traits of three distinct blood cell types—HSCs, erythroblasts (EBs), and megakaryocytes (MKs)—we conducted a comprehensive transcriptomic analysis. Leveraging diverse hematopoietic cell datasets from healthy individuals, sourced from The BLUEPRINT consortium, our investigation targeted the identification of genetic variants responsible for changes in gene expression levels and epigenetic modifications across the entire human genome in each of these cell types. The total number of normalized expressed transcripts includes 14,233 novel trinity lncRNAs, 13,749 mRNAs, and 3092 lncRNAs. This scrutiny revealed a total of 31,074 transcripts, with a notable revelation that 14,233 of them were previously unidentified or novel lncRNAs, highlighting a substantial reservoir of genetic information yet to be explored. Examining their expression across distinct lineages further unveiled 2845 differentially expressed (DE) mRNAs and 354 DE long noncoding RNAs (lncRNAs) notably enriched among the three distinct blood cell types: HSCs, EBs, and MKs. Our investigation extended beyond mRNA to focus on the dynamic expression of lncRNAs, revealing a well-defined pattern that played a significant role in regulating differentiation and cell-fate specification. This coordination of lncRNA dynamics extended to aberrations in both mRNA and lncRNA transcriptomes within HSCs, EBs, and MKs. We specifically characterized lncRNAs with preferential expression in HSCs, as well as in various downstream differentiated lineage progenitors of EBs and MKs, providing a comprehensive perspective on lncRNAs in human hematopoietic cells. Notably, the expression of lncRNAs exhibited substantial cell-to-cell variation, a phenomenon discernible only through single-cell analysis. The comparative analysis undertaken in this study provides valuable insights into the distinctive genetic signatures guiding the differentiation of these crucial hematopoietic cell types.
Effect of Different Glucose Levels and Glycation on Meningioma Cell Migration and Invasion
Authors: Selke Philipp , Strauss Christian , Horstkorte Rüdiger , Scheer Maximilian
Published: September 2024

Meningiomas are predominantly benign tumors, but there are also malignant forms that are associated with a poor prognosis. Like almost all tumors, meningiomas metabolize glucose as part of aerobic glycolysis (Warburg effect) for energy supply, so there are attempts to influence the prognosis of tumor diseases using a glucose-reduced diet. This altered metabolism leads to so called hallmarks of cancer, such as glycation and glycosylation. In this study, we investigated the influence of low (3 mM), normal (5.5 mM) and high glucose (15 mM) on a malignant meningioma cell line (IOMM-Lee, WHO grade 3). In addition, the influence of methylglyoxal, a by-product of glycolysis and a precursor for glycation, was investigated. Impedance-based methods (ECIS and RTCA) were used to study migration and invasion, and immunoblotting was used to analyze the expression of proteins relevant to these processes, such as focal adhesion kinase (FAK), merlin or integrin ß1. We were able to show that low glucose reduced the invasive potential of the cells, which was associated with a reduced amount of sialic acid. Under high glucose, barrier function was impaired and adhesion decreased, which correlated with a decreased expression of FAK.
Exogenous Melatonin Alleviates NaCl Injury by Influencing Stomatal Morphology, Photosynthetic Performance, and Antioxidant Balance in Maize
Authors: He Fuqiang , Zhao Xiaoqiang , Qi Guoxiang , Sun Siqi , Shi Zhenzhen , Niu Yining , Wu Zefeng , Zhou Wenqi
Published: September 2024

Maize (Zea mays L.) is sensitive to salt stress, especially during seed germination and seedling morphogenesis, which limits maize growth and productivity formation. As a novel recognized plant hormone, melatonin (MT) participates in multiple growth and developmental processes and mediates biotic/abiotic stress responses, yet the effects of salt stress on maize seedlings remain unclear. Herein, we investigated the effects of 150 μM exogenous MT on multiple phenotypes and physiologic metabolisms in three-leaf seedlings across eight maize inbred lines under 180 mM NaCl salt stress, including growth parameters, stomatal morphology, photosynthetic metabolisms, antioxidant enzyme activities, and reactive oxygen species (ROS). Meanwhile, the six gene expression levels controlling antioxidant enzyme activities and photosynthetic pigment biosynthesis in two materials with contrasting salt resistance were examined for all treatments to explore the possible molecular mechanism of exogenous MT alleviating salt injury in maize. The results showed that 150 μM exogenous MT application protected membrane integrity and reduced ROS accumulation by activating the antioxidant system in leaves of maize seedlings under salt stress, their relative conductivity and H2O2 level average reduced by 20.91% and 17.22%, while the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) averaged increased by 13.90%, 17.02%, 22.00%, and 14.24% relative to salt stress alone. The improvement of stomatal size and the deposition of photosynthetic pigments were more favorable to enhancing photosynthesis in leaves when these seedlings treated with MT application under salt stress, their stomatal size, chlorophyll content, and net photosynthetic rate averaged increased by 11.60%, 19.64%, and 27.62%. Additionally, Gene expression analysis showed that MT stimulation significantly increased the expression of antioxidant enzyme genes (Zm00001d009990, Zm00001d047479, Zm00001d014848, and Zm00001d007234) and photosynthetic pigment biosynthesis genes (Zm00001d011819 and Zm00001d017766) under salt stress. At the same time, 150 μM MT significantly promoted seedling growth and biomass accumulation. In conclusion, our study may unravel crucial evidence of the role of MT in maize seedlings against salt stress, which can provide a novel strategy for improving maize salt stress resistance.
Colchicine, Caffeine, Gramine, and Their Derivatives as Potential Herbicides, Fungicides, and Insecticides
Authors: Kurek Joanna , Sierakowska Arleta , Berdzik Natalia , Jasiewicz Beata
Published: September 2024

A preliminary in silico screening of 94 compounds, including colchicine, caffeine, gramine, and their derivatives, was conducted to identify potential herbicides, insecticides, and fungicides. Among the compounds tested, only gramine and its 13 derivatives exhibited potential activity. These compounds were further tested against eight species of insects, three species of weeds, and four species of fungi. All of the tested alkaloids were found to be ineffective as herbicides and insecticides, but they did exhibit some fungicidal activity. Four gramine derivatives showed some activity against Phytophthora infestans, Botrytis cinerea, Zymoseptoria tritici, and Fusarium culmorum.
Genus Ribes: Ribes aureum, Ribes pauciflorum, Ribes triste, and Ribes dikuscha—Comparative Mass Spectrometric Study of Polyphenolic Composition and Other Bioactive Constituents
Authors: Razgonova Mayya P. , Nawaz Muhammad Amjad , Sabitov Andrey S. , Golokhvast Kirill S.
Published: September 2024

This study presents the metabolomic profiles of the four Ribes species (Ribes pauciflorum Turcz., Ribes triste Pall., Ribes dicuscha Fisch., and Ribes aureum Purch.). The plant material was collected during two expeditions in the Russian Far East. Tandem mass spectrometry was used to detect target analytes. A total of 205 bioactive compounds (155 compounds from polyphenol group and 50 compounds from other chemical groups) were tentatively identified from the berries and extracts of the four Ribes species. For the first time, 29 chemical constituents from the polyphenol group were tentatively identified in the genus Ribes. The newly identified polyphenols include flavones, flavonols, flavan-3-ols, lignans, coumarins, stilbenes, and others. The other newly detected compounds in Ribes species are the naphthoquinone group (1,8-dihydroxy-anthraquinone, 1,3,6,8-tetrahydroxy-9(10H)-anthracenone, 8,8′-dihydroxy-2,2′-binaphthalene-1,1′,4,4′-tetrone, etc.), polyhydroxycarboxylic acids, omega-3 fatty acids (stearidonic acid, linolenic acid), and others. Our results imply that Ribes species are rich in polyphenols, especially flavanols, anthocyanins, flavones, and flavan-3-ols. These results indicate the utility of Ribes species for the health and pharmaceutical industry.
CD68-Negative Histiocytoses with Cardiac Involvement, Associated with COVID-19
Authors: Mitrofanova Lubov , Korneva Lubov , Makarov Igor , Bortsova Maria , Sitnikova Maria , Ryzhkova Daria , Kudlay Dmitry , Starshinova Anna
Published: September 2024

Histiocytoses are rare diseases characterised by infiltration of affected organs by myeloid cells with a monocyte or dendritic cell phenotype. Symptoms can range from self-resolving localised forms to multisystemic lesions requiring specific treatment. To demonstrate extremely rare cases of CD68-negative cardiac histiocytosis with expression of SARS-CoV-2 antigen in infiltrate cells. We demonstrated a case of Erdheim–Chester disease in a 67-year-old man with pericardial involvement and positive dynamics with vemurafenib treatment, an autopsy case of xanthogranulomatous myopericarditis in a 63-year-old man, surgical material of xanthogranulomatous constrictive pericarditis in a 57-year-old man, and an autopsy case of xanthogranulomatosis in a 1-month-old girl. In all cases, xanthogranuloma cells expressed CD163, many of them spike protein SARS-CoV-2, while CD68 expression was detected only in single cells. In this article, we demonstrated four cases of extremely rare CD68-negative cardiac xanthogranulomatosis in three adults and one child with expression of the spike protein SARS-CoV-2 in M2 macrophages. This potential indirect association between COVID-19 and the development of histiocytosis in these patients warrants further investigation. To substantiate this hypothesis, more extensive research is needed.
Molecular Aspects of the Interactions between Selected Benzodiazepines and Common Adulterants/Diluents: Forensic Application of Theoretical Chemistry Methods
Authors: Džodić Jelica , Marković Milica , Milenković Dejan , Dimić Dušan
Published: September 2024

Benzodiazepines are frequently encountered in crime scenes, often mixed with adulterants and diluents, complicating their analysis. This study investigates the interactions between two benzodiazepines, lorazepam (LOR) and alprazolam (ALP), with common adulterants/diluents (paracetamol, caffeine, glucose, and lactose) using infrared (IR) spectroscopy and quantum chemical methods. The crystallographic structures of LOR and ALP were optimized using several functionals (B3LYP, B3LYP-D3BJ, B3PW91, CAM-B3LYP, M05-2X, and M06-2X) combined with the 6-311++G(d,p) basis set. M05-2X was the most accurate when comparing experimental and theoretical bond lengths and angles. Vibrational and 13C NMR spectra were calculated to validate the functional’s applicability. The differences between LOR’s experimental and theoretical IR spectra were attributed to intramolecular interactions between LOR monomers, examined through density functional theory (DFT) optimization and quantum theory of atoms in molecules (QTAIM) analysis. Molecular dynamics simulations modeled benzodiazepine–adulterant/diluent systems, predicting the most stable structures, which were further analyzed using QTAIM. The strongest interactions and their effects on IR spectra were identified. Comparisons between experimental and theoretical spectra confirmed spectral changes due to interactions. This study demonstrates the potential of quantum chemical methods in analyzing complex mixtures, elucidating spectral changes, and assessing the structural stability of benzodiazepines in forensic samples.
Protective Effects of Crotonis Semen Extract against Sepsis through NF-κB Pathway Inhibition
Authors: Hwang Yo Sep , Yoon Hyang Ran , Park Hyo-Min , Jang Jun-Pil , Park Jun Hong , Park Seong-Hoon , Lim Jong Seok , Cho Hee Jun , Lee Hee Gu
Published: September 2024

Sepsis is an inflammatory condition causing organ failure due to an uncontrolled immune response to infection and remains a significant challenge. Crotonis Semen has displayed various pharmacological effects, yet its potential in protecting against sepsis and the mechanisms involved remains largely unclear. Here, we explored the antiseptic properties of Crotons Semen extract (CSE) in both LPS-stimulated J774 macrophages and mice subjected to sepsis through Cecal ligation and Puncture (CLP) or LPS induction. We found that CSE enhanced survival rates in mouse models with acute sepsis induced by CLP operation and LPS injection. Administering CSE also reduced levels of enzymes indicating organ damage, such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), and creatine kinase (CK), in septic mice. Furthermore, CSE lowered the serum levels of inflammatory mediators and cytokines, such as NO, TNF-α, IL-1β, and IL-6, in septic mice. In LPS-stimulated J774 macrophages, CSE reduced the expression of pro-inflammatory proteins, including iNOS and COX-2. Moreover, CSE inhibited the phosphorylation of IκBα and IKK, key components of the NF-κB signaling pathway, thereby reducing inflammatory mediators and cytokines. These results demonstrate CSE’s protective effects against sepsis through NF-κB pathway disruption, indicating its potential as a therapeutic option for acute inflammatory conditions.
Charting the Ethical Frontier in Newborn Screening Research: Insights from the NBSTRN ELSI Researcher Needs Survey
Authors: Unnikumaran Yekaterina , Lietsch Mei , Brower Amy
Published: September 2024

From 2008 to 2024, the Newborn Screening Translational Research Network (NBSTRN), part of the National Institute of Child Health and Human Development (NICHD) Hunter Kelly Newborn Screening Program, served as a robust infrastructure to facilitate groundbreaking research in newborn screening (NBS), public health, rare disease, and genomics. Over its sixteen years, NBSTRN developed into a significant international network, supporting innovative research on novel technologies to screen, diagnose, treat, manage, and understand the natural history of more than 280 rare diseases. The NBSTRN tools and resources were used by a variety of stakeholders including researchers, clinicians, state NBS programs, parents, families, and policy makers. Resources and expertise for the newborn screening community in ethical, legal, and social issues (ELSI) has been an important area of focus for the NBSTRN and this includes efforts across the NBS system from pilot studies of candidate conditions to public health implementation of screening for new conditions, and the longitudinal follow-up of NBS-identified individuals to inform health outcomes and disease understanding. In 2023, the NBSTRN conducted a survey to explore ELSI issues in NBS research, specifically those encountered by the NBS community. Since NBS research involves collaboration among researchers, state NBS programs, clinicians, and families, the survey was broadly designed and disseminated to engage all key stakeholders. With responses from 88 members of the NBS community, including researchers and state NBS programs, the survey found that individuals rely most on institutional and collegial resources when they encounter ELSI questions. Most survey responses ranked privacy as extremely or very important in NBS research and identified the need for policies that address informed consent in NBS research. The survey results highlight the need for improved collaborative resources and educational programs focused on ELSI for the NBS community. The survey results inform future efforts in ELSI and NBS research in the United States (U.S.) and the rest of the world, including the development of policies and expanded ELSI initiatives and tools that address the needs of all NBS stakeholders.
Preventing Dental Caries with Calcium-Based Materials: A Concise Review
Authors: Chen Jieyi , Zhang Yuqing , Yin Iris Xiaoxue , Yu Ollie Yiru , Chan Alice Kit Ying , Chu Chun Hung
Published: September 2024

This concise review provides an update on the use of calcium-based materials for the prevention of dental caries. Some calcium-based materials promote remineralization and neutralize bacterial acids, disrupting cariogenic biofilms and inhibiting bacterial growth. Medical Subject Headings of [Dental Caries] and [Calcium] were adopted to search publications. Information related to the aim of this review was extracted and summarized. Common calcium-based materials are calcium phosphate, hydroxyapatite, calcium carbonate, calcium fluoride and casein phosphopeptide–amorphous calcium phosphate (CPP-ACP). Calcium phosphate is commonly used in toothpaste. It provides calcium and phosphate ions, enhances the incorporation of fluoride into caries lesions and increases mineral density. Hydroxyapatite is a form of calcium phosphate that is chemically similar to the mineral found in teeth. It can be applied on teeth to prevent caries. Calcium carbonate can be found in toothpastes. It neutralizes bacterial acids and acts as a calcium reservoir during remineralization. Calcium fluoride is found in dental products and promotes remineralization as a source of fluoride, which can be incorporated into tooth enamel, forming fluorapatite and increasing resistance to caries. CPP-ACP is derived from milk proteins. It contains calcium and phosphate, which help to remineralize tooth enamel. CPP-ACP inhibits cariogenic bacteria. It also interacts with bacterial biofilms and disrupts their formation. These calcium-based materials can be used to boost the preventive effect of fluorides or, alternatively, as a therapy for caries prevention.
Eco-Morphological Responses of Camponotus japonicus (Hymenoptera: Formicidae) to Varied Climates and Habitats
Authors: Ma Ruoqing , Zhang Liangliang , He Hong
Published: September 2024

Ants are a highly adaptable group of insects that have globally established themselves in diverse climates and habitats. This study investigates the influence of climate and habitat on the morphological traits of Camponotus japonicus across 22 sites in mainland China. These sites span three climate zones (mid-temperate, warm temperate, and subtropical) and three habitat types (urban parks, farmlands, and sparse woodlands). Principal component analysis (PCA) was used to determine the principal axis of morphological variation, while hypervolume analysis and centroid distance calculation were used to verify the environmental filtering hypothesis and the optimal transfer hypothesis. The results support both hypotheses showing that climate and habitat significantly affect the morphological space of C. japonicus workers. In particular, the morphological space is more constrained in mid-temperate farmlands, while workers in sparse woodlands exhibit greater morphological variation. In contrast, urban parks are characterized by higher stability and reduced morphological differences. Additionally, robust regression analysis reveals that environmental factors such as temperature, precipitation, humidity, and altitude are closely linked to the morphological traits of the workers. Understanding how ant morphology responds to external environmental changes enhances our understanding of their adaptability and their essential ecological roles across various ecosystems.
Review of Existing Tools for Software Implementation of Digital Twins in the Power Industry
Authors: Iumanova Irina F. , Matrenin Pavel V. , Khalyasmaa Alexandra I.
Published: September 2024

Digital twin technology is an important tool for the digitalization of the power industry. A digital twin is a concept that allows for the creation of virtual copies of real objects that can be used for technical state analysis, predictive analysis, and optimization of the operation of power systems and their components. Digital twins are used to address different issues, including the management of equipment reliability and efficiency, integration of renewable energy sources, and increased flexibility and adaptability of power grids. Digital twins can be developed with the use of specialized software solutions for designing, prototyping, developing, deploying, and supporting. The existing diversity of software requires systematization for a well-informed choice of digital twin’s development tool. It is necessary to take into account the technical characteristics of power systems and their elements (equipment of power plants, substations and power grids of power systems, mini- and microgrids). The reviews are dedicated to tools for creating digital twins in the power industry. The usage of Digital Twin Definition Language for the description data of electromagnetic, thermal, and hydrodynamic models of a power transformer is presented.
Beyond Clinical Factors: Harnessing Artificial Intelligence and Multimodal Cardiac Imaging to Predict Atrial Fibrillation Recurrence Post-Catheter Ablation
Authors: Truong Edward T. , Lyu Yiheng , Ihdayhid Abdul Rahman , Lan Nick S. R. , Dwivedi Girish
Published: September 2024

Atrial fibrillation (AF) is the most common type of cardiac arrhythmia, with catheter ablation being a key alternative to medical treatment for restoring normal sinus rhythm. Despite advances in understanding AF pathogenesis, approximately 35% of patients experience AF recurrence at 12 months after catheter ablation. Therefore, accurate prediction of AF recurrence occurring after catheter ablation is important for patient selection and management. Conventional methods for predicting post-catheter ablation AF recurrence, which involve the use of univariate predictors and scoring systems, have played a supportive role in clinical decision-making. In an ever-changing landscape where technology is becoming ubiquitous within medicine, cardiac imaging and artificial intelligence (AI) could prove pivotal in enhancing AF recurrence predictions by providing data with independent predictive power and identifying key relationships in the data. This review comprehensively explores the existing methods for predicting the recurrence of AF following catheter ablation from different perspectives, including conventional predictors and scoring systems, cardiac imaging-based methods, and AI-based methods developed using a combination of demographic and imaging variables. By summarising state-of-the-art technologies, this review serves as a roadmap for developing future prediction models with enhanced accuracy, generalisability, and explainability, potentially contributing to improved care for patients with AF.
A Meta-Analysis Examining the Effect of Perioperative Biologic Disease-Modifying Anti-Rheumatic Medications on Postoperative Wound Complications in Various Orthopedic Surgeries
Authors: Imam Mohamed S. , Alshahrani Shahad A. , Alotaibi Rahaf Mubarak S. , Almurayeh Khalid Nassir , Alshabab Naif Ayidh , Almutairi Nora Khaled , Alomar Hanin , Alomair Leen , Boules Marina E. , Abdelrahim Mohamed E. , Amin Mohammed A.
Published: September 2024

This meta-analysis was designed to compare the risk of postoperative wound complications in various orthopedic surgeries (OSs) affected by the perioperative use of biologic disease-modifying anti-rheumatic drugs (bDMARDs). The odds ratio (OR) and mean difference (MD), with 95% confidence intervals (CIs), were calculated using dichotomous or continuous random or fixed-effect models, based on the meta-analysis data. This study incorporated 14 investigations conducted between 2005 and 2023, encompassing a total population of 19,021 individuals undergoing diverse OSs. Participants who continued their bDMARDs exhibited a substantially higher incidence of postoperative surgical site infections (OR, 1.39; 95% CI, 1.12–1.72, p = 0.002) compared to those who withheld bDMARDs. However, the study did not find any statistically significant difference between the continuation or withholding of bDMARDs regarding delayed wound healing (OR, 2.02; 95% CI, 1.00–4.06, p = 0.05) or disease flares (OR, 0.59; 95% CI, 0.28–1.25, p = 0.17). The results show that patients who continued their bDMARDs had a notably higher incidence of postoperative surgical site infections. However, no significant differences were observed in delayed wound healing or disease flares when compared to those who withheld bDMARDs. It is important to acknowledge the limitations of this analysis, such as the relatively small number of participants and the limited number of studies available for certain comparisons, which may impact the validity of the findings.
Left Atrial Volume versus Coronary Artery Calcium Score in Patients on Peritoneal Dialysis: An Observational Study
Authors: Reis Fabrício Moreira , Ferrari Eduarda Baccarin , Reis Nayrana Soares do Carmo , Costa Fabiana Lourenço , Morimoto Paula Naomi , Maurício Alejandra Del Carmen Villanueva , Hueb João Carlos , Bazan Rodrigo , Banin Vanessa Burgugi , Barretti Pasqual , Martin Luis Cuadrado , Zanati Bazan Silméia Garcia
Published: September 2024

Background: The coronary artery calcium score and left atrial volume have been shown to predict the incidence of acute myocardial infarction and death from cardiovascular disease in patients undergoing peritoneal dialysis. However, the association between these factors has not been well-established. Methods: This cross-sectional, prospective, single-center study was conducted on patients undergoing outpatient peritoneal dialysis, who were followed up at a university hospital between March 2018 and August 2019. The coronary artery calcium score was calculated based on cardiovascular computed tomography findings. The score was “positive” when it was ≥100 Agatston and “negative” when it was <100 Agatston. The left atrial volume was obtained using the biplane disc method at the end of the left ventricular systole, and then it was indexed to the body surface. Results: Forty-four patients were evaluated. They had an age [mean (range)] of 56 (43–65) years and had been on dialysis therapy for 11.7 (6.8–25.4) months. Univariate analysis revealed a relationship between the coronary artery calcium score and left atrial volume index and the following variables: age, diabetes, overhydration, pulse wave velocity, E/A ratio, and left ventricular mass index. In multivariate logistic regression analysis, only the left atrial volume index was independently associated with a positive coronary artery calcium score. Conclusions: The left atrial volume index was associated with a positive coronary artery calcium score in patients on peritoneal dialysis, regardless of other factors. It may be a useful risk marker for coronary artery disease in this population.
Heterotopic Ossifications Following Intramedullary Stabilization of Femoral Fractures in Polytraumatized Patients
Authors: Wollner Gregor , Hruska Florian , Koenig Felix R. M. , Haider Thomas , Negrin Lukas L.
Published: September 2024

Introduction: Heterotopic ossifications (HOs) are a well-known complication following total hip arthroplasty. Yet only little is known about the development of HOs following a femoral fracture and intramedullary stabilization in polytraumatized patients. Thus, the present study aimed to investigate whether the development of HOs is being observed more frequently in patients suffering polytrauma compared to those with single-extremity trauma. Materials and Methods: The retrospective outcome study was conducted at our level I trauma center. All patients admitted from 2010 to 2020 were included if they (1) presented with multiple injuries (≥2 body regions), (2) had an Injury Severity Score ≥16, (3) suffered a femoral fracture, and (4) were treated with intramedullary stabilization. Furthermore, a control group was established to match the polytrauma group (sex, age), who were suffering from single-extremity trauma (femoral fracture) which was treated with intramedullary stabilization. Subsequently, X-rays of the hip were performed and evaluated for up to one-year post-trauma. Results: Our study group consisted of 36 patients in total (91.7% male; mean age 39.4 ± 17.4 years, range: 18–82 years). The polytrauma (PT) group included 12 patients (mean age 39.5 years, median ISS 28), whereas the control group (single-extremity-trauma) included 24 patients (mean age 39.3 years). We documented HOs in nine (75%) patients in the PT group vs. five (20.8%) patients in the single-extremity group (p = 0.03). Conclusion: In this study, we were able to demonstrate that heterotopic ossifications are being observed significantly more frequently in patients suffering from polytrauma in comparison to patients with single-extremity trauma following intramedullary stabilization after a femoral fracture.
A Study on the Impact of Vertical Grid Parameter Perturbations in the Regional Ocean Modeling System
Authors: Wang Lei , Zhang Feng , Zheng Chongwei , Zhong Yaozhao , Lu Tianxiu , Shang Shaoping , Pu Siyu , Xia Guodong , Chen Huafei , Leng Wei
Published: September 2024

In this study, the Regional Ocean Modeling System (ROMS) is employed to construct a three-dimensional barotropic ocean model with a monodirectional upper boundary and homogeneous and steady wind covering the entire computation area. Eight perturbation experiments are designed to determine the vertical grid distribution difference with high resolution at the surface and bottom. Two types are considered in the model, including removing the Coriolis force (type 1) and employing a different Coriolis force (type 2). According to the experiments, the velocity of the current in type 1 yields uncertainty, and wind energy could penetrate the upper ocean and reach the abyss. The surface velocity in type 2 is fundamentally compatible with the empirical relationship constructed by Ekman, and the curved lines of the vertical distribution of horizontal currents nearly match. For type 1, the velocity is very strong from the sea surface to the bottom. When comparing type 1 and type 2 cases, the Coriolis force obstructs the wind energy transfer into the deep ocean. In addition, the European Centre for Medium-Range Weather Forecasts (ECMWF)’s global surface wind distribution indicates that the realistic ocean upper wind boundary is similar to the numerical experiment in the Pacific and Atlantic oceans, where the wind direction is along the latitude line at the equator. In order to make the experimental situation as close as possible to the real ocean, validation experiments are conducted in this study to consider the uncertainty in the current profile at the equator. The simulation results of type 1 differ significantly from the data obtained from the real ocean. This uncertainty may transfer the signal to higher latitudes, causing incorrect simulation results, especially in the critical region. Overall, this research not only makes discoveries in physical ocean theory but also guides predictive and forecasting techniques for ocean modeling.
Intelligent Fault Diagnosis Method for Constant Pressure Variable Pump Based on Mel-MobileViT Lightweight Network
Authors: Zhao Yonghui , Jiang Anqi , Jiang Wanlu , Yang Xukang , Xia Xudong , Gu Xiaoyang
Published: September 2024

The sound signals of hydraulic pumps contain abundant key information reflecting their internal mechanical states. In environments characterized by high temperatures or high-speed rotation, or where sensor deployment is challenging, acoustic sensors offer non-contact and flexible arrangement features. Therefore, this study aims to develop an intelligent fault diagnosis method for hydraulic pumps based on acoustic signals. Initially, the Adaptive Chirp Mode Decomposition (ACMD) method is employed to remove environmental noise from the acoustic signals, enhancing the feature signals. Subsequently, the Mel spectrum is extracted as the acoustic fingerprint features of various fault states of the hydraulic pump, and these features are used to train the MobileViT network, achieving accurate identification of the different fault modes. The results indicate that the proposed Mel-MobileViT model effectively identifies and classifies various faults in constant pressure variable pumps, outperforming other models. This study not only provides an efficient and reliable intelligent method for the fault diagnosis of critical industrial equipment such as hydraulic pumps, but also offers new perspectives on the application of deep learning in acoustic pattern analysis.
The Impact of Frailty and Gender Differences on Hospitalization and Complications in Proximal Femoral Pathological Fractures: A Cross-Sectional Study
Authors: El Motassime Alessandro , Pesare Elisa , Russo Andrea , Salini Sara , Gava Giordana , Recupero Carla , Giani Tommaso , Covino Marcello , Maccauro Giulio , Vitiello Raffaele
Published: September 2024

Background: Frailty associated with aging increases the risk of falls, disability, and death. The aim of this study is to explore gender-related disparities in the survival outcomes of pathological femoral fractures in older frail patients, while analyzing potential specific prognostic factors. Methods: This study is a retrospective observational analysis conducted at a single medical center. It enrolled all patients aged 65 and above who were admitted to our emergency department between 2016 and 2020 with a diagnosis of pathological femur fracture requiring surgical intervention. The primary study endpoint was evaluating gender-related differences in survival outcomes. The secondary endpoint involves investigating gender-specific prognostic factors through the analysis of clinical and laboratory parameters. Results: The average Charlson Comorbidity Index (CCI) was slightly lower in men, but the difference was not statistically significant (p = 0.53). The Clinical Frailty Scale (CFS) showed similar results, with men and women 5.23 (SD 1.46), also not significant (p = 0.83). An evaluation comparing patients aged 75 years or younger to those older than 75 years found significant differences in health metrics. The average CCI was higher in the over 75 group compared to the under 75 group, with a p-value of 0.001. Similarly, the CFS average was also greater in the over 75 group than in the under 75 group, with a p-value of 0.0001. Complications were more frequent in patients over 75 and those with lower educational qualifications. The evaluation analyzed cardiac patients compared to a control group, revealing that the average age of cardiac patients was 75.22 years, while the control group was younger at 73.98 years (p = 0.5119). The CCI for cardiac patients averaged 6.53, significantly higher than 4.43 for non-cardiac patients (p = 0.0003). Conclusion: Frailty assessment is therefore essential in patients with pathological fracture of the proximal femur and is an important predictor of both gender differences and hospital complications. Enhancing gender analysis in this field is crucial to gather more robust evidence and deeper comprehension of potential sex- and gender-based disparities.
Effectiveness and Safety of mRNA Vaccines in the Therapy of Glioblastoma
Authors: Strika Zdeslav , Petković Karlo , Likić Robert
Published: September 2024

Glioblastoma (GBM) is the most common and most malignant primary brain tumor, presenting significant treatment challenges due to its heterogeneity, invasiveness, and resistance to conventional therapies. Despite aggressive treatment protocols, the prognosis remains poor, with a median survival time of approximately 15 months. Recent advancements in mRNA vaccine technology, particularly the development of lipid nanoparticles (LNPs), have revitalized interest in mRNA-based therapies. These vaccines offer unique advantages, including rapid production, personalization based on tumor-specific mutations, and a strong induction of both humoral and cellular immune responses. mRNA vaccines have demonstrated potential in preclinical models, showing significant tumor regression and improved survival rates. Early-phase clinical trials have indicated that mRNA vaccines are safe and can induce robust immune responses in GBM patients. Combining mRNA vaccines with other immunotherapeutic approaches, such as checkpoint inhibitors, has shown synergistic effects, further enhancing their efficacy. However, challenges such as optimizing delivery systems and overcoming the immunosuppressive tumor microenvironment remain. Future research should focus on addressing these challenges and exploring combination therapies to maximize therapeutic benefits. Large-scale, randomized clinical trials are essential to validate the efficacy and safety of mRNA vaccines in GBM therapy. The potential to reshape the tumor microenvironment and establish long-term immunological memory underscores the transformative potential of mRNA vaccines in cancer immunotherapy.
Multi-Scenario Simulation of Land-Use/Land-Cover Changes and Carbon Storage Prediction Coupled with the SD-PLUS-InVEST Model: A Case Study of the Tuojiang River Basin, China
Authors: Wang Qi , Zhang Wenying , Xia Jianguo , Ou Dinghua , Tian Zhaonan , Gao Xuesong
Published: September 2024

Land-use and land-cover changes (LUCCs) significantly impact carbon sequestration by modifying the structure and function of terrestrial ecosystems. This study utilized GIS and remote sensing techniques to forecast future LUCC patterns and their influence on regional carbon budgets, which is essential for sustainable development. We devised a coupled system dynamics (SD) model integrated with a patch-generating land-use simulation (PLUS) model to simulate LUCCs under diverse future scenarios using multisource environmental data. Additionally, the InVEST model was employed to quantify carbon storage in terrestrial ecosystems. By establishing three scenarios—ecological priority (EP), highly urbanized (HU), and coordinated development (CD)—this study’s aim was to predict the LUCC patterns and carbon storage distribution of the Tuojiang River Basin (TRB), China, up to 2035. The results showed that (1) from 2000 to 2020, significant LUCCs occurred in the TRB, primarily involving the conversion of cultivated land into construction areas and forestland; (2) LUCCs had a substantial impact on carbon storage in the TRB, with the EP scenario demonstrating the highest carbon storage by 2035 due to extensive forest expansion, while the HU scenario indicated a decline in carbon storage associated with rapid urbanization; and (3) the mountainous regions of the TRB, dominated by forestland, consistently exhibited higher carbon storage, whereas the Chengdu Plain region in the upper basin displayed the lowest. In conclusion, we recommend prioritizing the CD scenario in future development strategies to balance economic growth with ecological protection while simultaneously enhancing carbon storage. Our findings offer valuable insights to shape future LUCC policies in the Tuojiang River Basin, underscoring the adaptability of the coupled model approach to a wide range of geographic scales and contexts.
Bilingualism of Children in Different Multilingual Contexts
Authors: Nocus Isabelle
Published: September 2024

Many parents and professionals believe that learning to speak, read and write in two languages can lead to academic deficiencies due to cognitive overload and the risk of confusion linked to handling two language codes. Therefore, some bilinguals abandon or are tempted to abandon one of the two languages, often the first language, in exchanges with their children, in favor of the language of schooling. However, all recent scientific data tend to show that bilingualism is an asset more than a handicap. Nevertheless, these positive results most often concern English-speaking contexts and are not directly transposable to a French-speaking context. Drawing on the results of our work carried out in Oceania and in other territories, this article will deal with bilingual development and the impact of educational systems that promote the heritage or local languages from primary school. More specifically, the oral language of the bilingual, biliteracy and the effects of cross-linguistic transfer will be addressed. Results from both longitudinal studies in New Caledonia and French Polynesia, confirmed by other studies conducted in Sub-Saharan African, show a positive effect of the bilingual education curriculum on local language (Drehu and Tahitian) skills without having negative effects on French. We demonstrated that the expected effects of cross-linguistic transfer are only possible if the pupils learn to read and write in the two languages (local language and French). Additionally, learning to read in one of those local languages makes it easier to learn to read in French, which has a more opaque writing system.
Human Rights at the Climate Crossroads: Analysis of the Interconnection between Human Rights, Right to Climate, and Intensification of Extreme Climate Events
Authors: Díaz-Cruces Eliana , Méndez Rocasolano María , Zamora-Ledezma Camilo
Published: September 2024

This paper analyzes the theoretical foundation and practical implications of recognizing the right to a stable climate as a fundamental human right. Further, it examines the intersection of human rights, right to climate, and the intensification of extreme climate events. Through a bibliometric analysis, the study highlights the increase in scholarly attention paid to this nexus. The intensification of extreme climate events, such as the Cumbre Vieja volcano in Spain, is also analyzed as a catalyst for recognizing the right to climate as a human right, as a fundamental requirement for its enactment. Indeed, it is argued that this recognition is necessary to achieve climate justice. These thoughts about the necessity of recognizing the right to climate as a human right are also based on a similar case, the enactment of the rights to water and sanitation, which is presented as a case study, demonstrating how specific environmental rights can be integrated into human rights discourse. The results and discussion section synthesizes these findings, highlighting the imperative of recognizing climate rights to ensure justice and sustainability amidst escalating climate challenges.
Management of Extra-Articular and Intra-Articular Distal Radius Malunion
Authors: Liu Ting-Yu , Yang Chen-Yuan
Published: September 2024

This article presents a comprehensive overview of managing extra-articular and intra-articular distal radius malunions (DRM), discussing the pathoanatomy, clinical, and radiologic evaluation, conservative treatment, and surgical strategies. Corrective osteotomy remains the primary surgical intervention for symptomatic DRM. Surgical planning should consider factors such as timing, approach, correction technique, implant, graft, and associated injuries. The correction of extra-articular malunion necessitates brachioradialis tenotomy, circumferential periosteum release, and intrafocal elevation with grafting to facilitate distal radius realignment following osteotomy. Computer-assisted planning with 3D-printed patient-specific instrumentation (PSI) could help execute extra-articular osteotomy with high precision. As for the management of intra-articular malunion, it may require wrist arthrotomy, arthroscopy, or PSI assistance for precise articular osteotomy and reduction of the joint surface. This review highlights the importance of early intervention, thorough preoperative planning, and appropriate surgical techniques to optimize outcomes and minimize complications. Future research should focus on large-scale randomized controlled trials to compare different surgical methods, particularly for intra-articular DRM.
HLA-C Peptide Repertoires as Predictors of Clinical Response during Early SARS-CoV-2 Infection
Authors: Olp Michael D. , Laufer Vincent A. , Valesano Andrew L. , Zimmerman Andrea , Woodside Kenneth J. , Lu Yee , Lauring Adam S. , Cusick Matthew F.
Published: September 2024

The human leukocyte antigen (HLA) system plays a pivotal role in the immune response to viral infections, mediating the presentation of viral peptides to T cells and influencing both the strength and specificity of the host immune response. Variations in HLA genotypes across individuals lead to differences in susceptibility to viral infection and severity of illness. This study uses observations from the early phase of the COVID-19 pandemic to explore how specific HLA class I molecules affect clinical responses to SARS-CoV-2 infection. By analyzing paired high-resolution HLA types and viral genomic sequences from 60 patients, we assess the relationship between predicted HLA class I peptide binding repertoires and infection severity as measured by the sequential organ failure assessment score. This approach leverages functional convergence across HLA-C alleles to identify relationships that may otherwise be inaccessible due to allelic diversity and limitations in sample size. Surprisingly, our findings show that severely symptomatic infection in this cohort is associated with disproportionately abundant binding of SARS-CoV-2 structural and non-structural protein epitopes by patient HLA-C molecules. In addition, the extent of overlap between a given patient’s predicted HLA-C and HLA-A peptide binding repertoires correlates with worse prognoses in this cohort. The findings highlight immunologic mechanisms linking HLA-C molecules with the human response to viral pathogens that warrant further investigation.
Vector-Borne Zoonotic Lymphadenitis—The Causative Agents, Epidemiology, Diagnostic Approach, and Therapeutic Possibilities—An Overview
Authors: Oršolić Martina , Sarač Nikolina , Balen Topić Mirjana
Published: September 2024

In addition to common skin pathogens, acute focal lymphadenitis in humans can, in rare cases, be caused by a zoonotic pathogen. Furthermore, it can develop in the absence of any direct or indirect contact with infected animals, in cases when the microorganism is transmitted by a vector. These clinical entities are rare, and therefore often not easily recognized, yet many zoonotic illnesses are currently considered emerging or re-emerging in many regions. Focal zoonotic vector-borne lymphadenitis and its numerous causative agents, with their variegated clinical manifestations, have been described in some case reports and small case series. Therefore, we summarized those data in this narrative overview, with the aim of raising clinical awareness, which could improve clinical outcomes. This overview briefly covers reported pathogens, their vectors and geographic distribution, and their main clinical manifestations, diagnostic possibilities, and recommended therapy. Vector-borne tularemia, plague, bartonellosis, rickettsioses, borreliosis, and Malayan filariasis are mentioned. According to the existing data, when acute focal bacterial vector-borne zoonotic lymphadenitis is suspected, in severe or complicated cases it seems prudent to apply combined aminoglycoside (or quinolone) plus doxycycline as an empirical therapy, pending definite diagnostic results. In this field, the “one health approach” and further epidemiological and clinical studies are needed.