bluuebunny/crossref_metadata_2025 huggingface dataset:
Antioxidative potential and ameliorative effects of green lentil (Lens culinaris M.) sprouts against CCl4-induced oxidative stress in rats
Authors: Barakat Hassan , Alshimali Saleh I. , Almutairi Abdulkarim S. , Alkhurayji Raghad I. , Almutiri Sarah M. , Aljutaily Thamer , Algheshairy Reham M. , Alhomaid Raghad M. , Aljalis Rashed A. , Alkhidhr Mohammed F. , Abdellatif Ahmed A. H.
Published: November 2022

The present study is aimed to investigate the antioxidative potential and ameliorative effects of Lens culinaris Medikus sprouts hydroalcoholic extract (LSHE) on CCl4-induced oxidative stress in rats. The research has been carried out in two successive stages. Firstly, the highest phenolic content and antioxidant activity of L. culinaris sprouts were assessed at 20 ± 1°C and 90–93% RH during sprouting. Total phenolic content (TPC), total carotenoids (TC), total flavonoids (TF), total flavonols (TFL), DPPH-RSA, and vitamin C contents of L. culinaris seeds and 6-days sprouts were determined. Subsequently, phenolics by HPLC analysis of L. culinaris seeds, 3rd and 6th-day sprouts were identified and quantified. Results indicated that 6th-day sprouts contained considerable phenolics with superior antioxidant capacity, thus selected to be examined for biological activity in a rat's module consisting of five groups. G1, normal rats orally received distilled water. G2 received 1.0 mL kg−1 of CCl4 and olive oil (1:1) intraperitoneally (i.p.) twice a week. G3 received CCl4 (i.p.) and 50 mg GAE kg−1 of LSHE daily/orally. G4 received CCl4 (i.p.) 100 mg kg−1 of LSHE orally/daily. G5 (reference group) treated by intramuscular injection (i.m.) of vit. E+Selenium (Vit. E+Se, 50 mg kg−1 twice a week). The weight gain, relative weight of organs, hypoglycemic and hypolipidemic efficiencies, liver's and kidneys' functions, and antioxidant biomarkers were examined. LSHE enhanced the weight gain recovery % and significantly reduced fasting blood glucose. The hypolipidemic effect of LSHE was dramatically reduced triglycerides (TG), total cholesterol (CHO), high- and low-density lipoproteins (HDL-c and LDL-c), and very-low-density lipoproteins (VLDL-c). Administration of 50 and 100 LSHE mg kg−1 ameliorated liver and kidney function in dose-dependent manure. Intriguingly, LSHE considerably reduced malondialdehyde (MDA) while significantly raising reduced glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) in a dose-dependent manner. In conclusion, biochemical examinations confirmed the therapeutic efficacy of LSHE as a functional product. It encouraged us to recommend L. culinaris sprout production for attenuating hepatotoxicity and nephrotoxicity, as well as being beneficial and profitable for controlling oxidative stress complications.
Proteoglycan-4 predicts good prognosis in patients with hepatocellular carcinoma receiving transcatheter arterial chemoembolization and inhibits cancer cell migration in vitro
Authors: Guo Yuan , Hu Hong Tao , Xu Shi Jun , Xia Wei Li , Zhao Yan , Zhao Xiao Hui , Zhu Wen Bo , Li Fang Ting , Li Hai Liang
Published: November 2022

Purpose

To search for adaptive response molecules that affect the efficacy of transcatheter arterial chemoembolization (TACE), analyze their clinical correlation with and prognostic value for hepatocellular carcinoma (HCC), and explore their impact on cell biological behavior and their mechanisms of action.

Methods

HCC tissue gene sequencing was used to identify differentially expressed genes. The expression of proteoglycan 4 (PRG4) in the serum of 117 patients with HCC who received TACE was detected by enzyme-linked immunosorbent assay. Serum-free medium mimicked TACE-induced nutrient deprivation. Cells with stable knockdown of PRG4 (shPRG4) were constructed to verify the effect and mechanism of PRG4 on the biological behavior of HCC cells in vitro.

Results

The expression of PRG4 was significantly elevated under TACE-induced starvation conditions. Low PRG4 expression was associated with worse response to TACE treatment, shorter survival time, and stronger HCC migration ability. Furthermore, in vitro experiments showed that knockdown of PRG4 promoted HCC cell migration by enhancing epithelial–mesenchymal transition (EMT) while did not affect proliferation. When PRG4 expression was low, starvation treatment impaired the migratory ability of HCC cells and reduced the chemosensitivity of HCC cells to epirubicin.

Conclusions

PRG4 expression predicts survival and TACE treatment response in patients with HCC. Furthermore, knockdown of PRG4 enhanced EMT, leading to HCC cell migration. PRG4 may serve as a biomarker for HCC patients receiving TACE.
PD-L1 expression and association with genetic background in pheochromocytoma and paraganglioma
Authors: Hadrava Vanova Katerina , Uher Ondrej , Meuter Leah , Ghosal Suman , Talvacchio Sara , Patel Mayank , Neuzil Jiri , Pacak Karel
Published: November 2022

Metastatic pheochromocytomas and paragangliomas (PPGLs) are rare neuroendocrine tumors associated with poor prognosis and limited therapeutic options. Recent advances in oncology-related immunotherapy, specifically in targeting of programmed cell death-1 (PD-1)/programmed death-ligand 1 (PD-L1) pathways, have identified a new treatment potential in a variety of tumors, including advanced and rare tumors. Only a fraction of patients being treated by immune checkpoint inhibitors have shown to benefit from it, displaying a need for strategies which identify patients who may most likely show a favorable response. Building on recent, promising outcomes in a clinical study of metastatic PPGL using pembrolizumab, a humanized IgG4κ monoclonal antibody targeting the PD-1/PD-L1 pathway, we examined PD-L1 and PD-L2 expression in relation to oncogenic drivers in our PPGL patient cohort to explore whether expression can predict metastatic potential and/or be considered a predictive marker for targeted therapy. We evaluated RNA expression in the NIH cohort of 48 patients with known genetic predisposition (sporadic; pseudohypoxia: SDHB, VHL, EPAS1, EGLN1; kinase signaling: RET, NF1) and 6 normal medulla samples (NAM). For comparison, 72 PPGL samples from The Cancer Genome Atlas (TCGA) were used for analysis of gene expression based on the variant status (pseudohypoxia: SDHB, VHL, EPAS1, EGLN1; kinase signaling: NF1, RET). Expression of PD-L1 was elevated in the PPGL cohort compared to normal adrenal medulla, aligning with the TCGA analysis, whereas PD-L2 was not elevated. However, expression of PD-L1 was lower in the pseudohypoxia cluster compared to the sporadic and the kinase signaling subtype cluster, suggesting that sporadic and kinase signaling cluster PPGLs could benefit from PD-1/PD-L1 therapy more than the pseudohypoxia cluster. Within the pseudohypoxia cluster, expression of PD-L1 was significantly lower in both SDHB- and non-SDHB-mutated tumors compared to sporadic tumors. PD-L1 and PD-L2 expression was not affected by the metastatic status. We conclude that PD-L1 and PD-L2 expression in our cohort of PPGL tumors was not linked to metastatic behavior, however, the presence of PPGL driver mutation could be a predictive marker for PD-L1-targeted therapy and an important feature for further clinical studies in patients with PPGL.
Stage IV non-small cell lung cancer among young individuals: Incidence, presentations, and survival outcomes of conventional therapies
Authors: Cai Jing-Sheng , Qiu Man-Tang , Yang Fan , Wang Xun
Published: November 2022

Background

There is a paucity of data published on the clinicopathological features and prognosis of stage IV non-small cell lung cancer (NSCLC) patients aged ≤45 years. Herein, we evaluated a large clinical series in an effort to provide a clearer picture of this population.

Methods

The least absolute shrinkage and selection operator (LASSO)-penalized Cox regression model was performed to identify prognostic factors for NSCLC among individuals aged ≤45 years. The Kaplan–Meier method with log-rank test was used to compare overall survival (OS) differences between groups. Competing risk analysis with the Fine–Gray test was used to analyze cancer-specific survival (CSS) differences. Propensity score matching (PSM) was used to minimize selection bias.

Results

Incidence-rate analyses, including 588,680 NSCLC cases (stage IV, 233,881; age ≤ 45 years stage IV, 5,483; and age > 45 years stage IV, 228,398) from 2004 to 2015, showed that the incidence of stage IV NSCLC among young individuals decreased over the years. In comparative analyses of clinical features and survival outcomes, a total of 48,607 eligible stage IV cases (age ≤ 45 years stage IV, 1,390; age > 45 years stage IV, 47,217) were included. The results showed that although patients in the young cohort were more likely to be diagnosed at advanced stages, they were also more likely to receive aggressive treatments. In addition, the survival rates of the young patients were superior to those of the older patients both before and after PSM.

Conclusions

Stage IV NSCLC patients aged ≤45 years comprise a relatively small but special NSCLC subgroup. Although this population had better survival outcomes than older patients, these patients deserve more attention due to their young age and the significant socioeconomic implications.
Characterization of genomic alterations and neoantigens and analysis of immune infiltration identified therapeutic and prognostic biomarkers in adenocarcinoma at the gastroesophageal junction
Authors: Lao Yueqiong , Wang Yuqian , Yang Jie , Liu Tianyuan , Ma Yuling , Luo Yingying , Sun Yanxia , Li Kai , Zhao Xuan , Niu Xiangjie , Xi Yiyi , Zhong Ce
Published: November 2022

Objectives

Adenocarcinoma at the gastroesophageal junction (ACGEJ) refers to a malignant tumor that occurs at the esophagogastric junction. Despite some progress in targeted therapies for HER2, FGFR2, EGFR, MET, Claudin 18.2 and immune checkpoints in ACGEJ tumors, the 5-year survival rate of patients remains poor. Thus, it is urgent to explore genomic alterations and neoantigen characteristics of tumors and identify CD8+ T-cell infiltration-associated genes to find potential therapeutic targets and develop a risk model to predict ACGEJ patients’ overall survival (OS).

Methods

Whole-exome sequencing (WES) was performed on 55 paired samples from Chinese ACGEJ patients. Somatic mutations and copy number variations were detected by Strelka2 and FACETS, respectively. SigProfiler and SciClone were employed to decipher the mutation signature and clonal structure of each sample, respectively. Neoantigens were predicted using the MuPeXI pipeline. RNA sequencing (RNA-seq) data of ACGEJ samples from our previous studies and The Cancer Genome Atlas (TCGA) were used to identify genes significantly associated with CD8+ T-cell infiltration by weighted gene coexpression network analysis (WGCNA). To construct a risk model, we conducted LASSO and univariate and multivariate Cox regression analyses.

Results

Recurrent MAP2K7, RNF43 and RHOA mutations were found in ACGEJ tumors. The COSMIC signature SBS17 was associated with ACGEJ progression. CCNE1 and VEGFA were identified as putative CNV driver genes. PI3KCA and TP53 mutations conferred selective advantages to cancer cells. The Chinese ACGEJ patient neoantigen landscape was revealed for the first time, and 58 potential neoantigens common to TSNAdb and IEDB were identified. Compared with Siewert type II samples, Siewert type III samples had significant enrichment of the SBS17 signature, a lower TNFRSF14 copy number, a higher proportion of samples with complex clonal architecture and a higher neoantigen load. We identified 10 important CD8+ T-cell infiltration-related Hub genes (CCL5, CD2, CST7, GVINP1, GZMK, IL2RB, IKZF3, PLA2G2D, P2RY10 and ZAP70) as potential therapeutic targets from the RNA-seq data. Seven CD8+ T-cell infiltration-related genes (ADAM28, ASPH, CAMK2N1, F2R, STAP1, TP53INP2, ZC3H3) were selected to construct a prognostic model. Patients classified as high risk based on this model had significantly worse OS than low-risk patients, which was replicated in the TCGA-ACGEJ cohort.

Conclusions

This study provides new neoantigen-based immunotherapeutic targets for ACGEJ treatment and effective disease prognosis biomarkers.
Methylation-based reclassification and risk stratification of skull-base chordomas
Authors: Huo Xulei , Guo Tengxian , Wang Ke , Yao Bohan , Li Da , Li Huan , Chen Wei , Wang Liang , Wu Zhen
Published: November 2022

Background

Skull-base chordomas are rare malignant bone cancers originating from the remnant of the notochord. Survival is variable, and clinical or molecular factors cannot reliably predict their outcomes. This study therefore identified epigenetic subtypes that defined new chordoma epigenetic profiles and their corresponding characteristics.

Methods

Methylation profiles of 46 chordoma-resected neoplasms between 2008 and 2014, along with clinical information, were collected. K-means consensus clustering and principal component analysis were used to identify and validate the clusters. Single-sample gene set enrichment analysis, methylCIBERSORT algorithm, and copy number analysis were used to identify the characteristics of the clusters.

Results

Unsupervised clustering analysis confirmed two clusters with a progression-free survival difference. Gene set enrichment analysis indicated that the early and late estrogen response pathways and the hypoxia pathway were activated whereas the inflammatory and interferon gamma responses were suppressed. Forty-six potential therapeutic targets corresponding to differentially methylated sites were identified from chordoma patients. Subgroups with a worse outcome were characterized by low immune cell infiltration, higher tumor purity, and higher stemness indices. Moreover, copy number amplifications mostly occurred in cluster 1 tumors and the high-risk group. Additionally, the presence of a CCNE1 deletion was exclusively found in the group of chordoma patients with better outcome, whereas RB1 and CDKN2A/2B deletions were mainly found in the group of chordoma patients with worse outcome.

Conclusions

Chordoma prognostic epigenetic subtypes were identified, and their corresponding characteristics were found to be variable.
Osteofibrous dysplasia-like adamantinoma: A case report and literature review
Authors: Li Jian-Wei , Miao Lei , Zhao Zhen-Guo , Yang Lin , Shi Zhuo , Li Meng
Published: November 2022

Abstract background

Osteofibrous dysplasia-like adamantinoma (OFD-like adamantinoma), classical adamantinoma and dedifferentiated adamantinoma were previously considered to be three subtypes of adamantinoma of long bones. In the 5th edition of the World Health Organization (WHO) classification of bone tumors in 2020, OFD-like adamantinoma was newly proposed and classified as an intermediate-locally aggressive tumor in other mesenchymal tumors of bone. OFD-like adamantinoma is rare, accounting for only 0.4% of all primary bone tumors. OFD-like adamantinoma is often misdiagnosed due to the insufficient understanding of it. Here we report a case of OFD-like adamantinoma treated in our hospital with a literature review.

Case presentation

The patient, a 14-year-old male, had swelling in his right leg with intermittent pain for one year. Plain radiography, computed tomography (CT) and magnetic resonance imaging (MRI) were performed. Based on the radiological and histological examinations, a diagnosis of OFD-like adamantinoma was suspected. After admission, the patient underwent tumor resection of the right tibia, free transplantation of the left fibula and internal fixation. After resection of the tumor, the wound recovered well, the vital signs were stable, and activity was normal. The patient has been followed up for more than a year with no recurrence or distant metastasis.

Conclusion

OFD-like adamantinoma is a rare primary bone tumor with nonspecific clinical features. Imaging examination can demonstrate the lesion and help diagnosis. The pathological discovery of epithelioid tissue is the key evidence for diagnosis.
Efficacy of transdermal flunixin in mitigating castration pain in piglets
Authors: Lopez-Soriano Magdiel , Rocha Merenda Victoria , Esteves Trindade Pedro Henrique , Loureiro Luna Stelio Pacca , Pairis-Garcia Monique Danielle
Published: November 2022

Castration is a painful procedure performed in swine and to date, there are no approved products available in the US to alleviate this pain. Previous work evaluating the efficacy of flunixin meglumine has shown promise in mitigating pain in swine, but no work to date has evaluated transdermal flunixin efficacy in mitigating castration pain in piglets. Therefore, the objective of this study was to evaluate the efficacy of transdermal flunixin (TDF) in mitigating castration pain utilizing a previously validated behavioral pain scale. A total of 98 Large White x Duroc cross male piglets from 98 litters were enrolled in this study. Piglets were randomly assigned to the following treatments: (1) TDF plus castration (3.33 mg/kg; CF;n = 24), (2) TDF plus sham castration (3.33 mg/kg; SF;n = 26), (3) topical physiological saline plus sham castration (S;n = 24), or (4) topical physiological saline plus castration (C;n = 24). All treatments were administered 24 h prior to castration. Four-min continuous videos clips were collected 24 h before castration (−24 h), immediately post-castration (0 h), and 24 h post-castration (+24 h). Video clips were then observed and scored by one trained observer using a 4-point pain scale (score 0–3) encompassing the five behavioral domains of the pig acute pain scale (UPAPS). Total pain score averages were analyzed as repeated measures by analysis of variance applying a multilevel model. The UPAPS effectively distinguished varying levels of painful and non-painful states in castrated piglets as observedviadeviations in total pain scores across timepoints (P < 0.0001), treatment (P < 0.001) and treatment*timepoint (P < 0.0001). Immediately post-castration (0 h), piglets in the C and CF group demonstrated greater total average pain scores than piglets in the S (P < 0.03) and SF (P < 0.01) groups and castrated piglets treated with TDF demonstrated lower total pain scores (P < 0.05) and required less analgesic intervention immediately post-castration compared to castrated piglets receiving no treatment (P < 0.0001). For C group 54% required rescue analgesia compared to 29%, 8% and 0% for CF, SF and S piglets respectively. Future work should evaluate implementation of this pain management protocol on a wide scale commercial farm setting.
Carrier frequency and incidence estimation of familial hemophagocytic lymphohistiocytosis in East Asian populations by genome aggregation database (gnomAD) based analysis
Authors: Park Jong Eun , Lee Taeheon , Ha Kyeongsu , Cho Eun Hye , Ki Chang-Seok
Published: November 2022

Objectives

Hemophagocytic lymphohistiocytosis (HLH) is a clinical syndrome characterized by a life-threatening condition caused by severe hypercytokinemia. The hereditary forms of HLH, also called familial HLH (fHLH), have 4 different genes (PRF1, UNC13D, STX11, and STXBP2) and have been identified as being causative for fHLH. This study aimed to analyze the carrier frequency and expected incidence of fHLH in East Asians and Koreans using exome data from the Genome Aggregation Database (gnomAD).

Methods

We analyzed 9,197 exomes for East Asian populations from gnomAD with 1,909 Korean for four fHLH genes. All identified variants were classified according to 2015 American College of Medical Genetics and Genomics and the Association for Molecular Pathology guideline.

Results

19 pathogenic variant/likely pathogenic variants (PV/LPVs) were identified in 30 East Asian individuals (0.33%). Among them, 7 PV/LPVs were identified in 17 Korean individuals (0.63%). The estimated incidence of fHLH was 1 in 1,105,652 for East Asians and l in 235,128 for Koreans.

Conclusions

This study is the first to identify carrier frequencies in East Asian and Korean populations for fHLH using gnomAD. It was confirmed that the carrier frequency of fHLH patients was high in Koreans among East Asians and the incidence was also predicted to be higher than that of other East Asians. The variant spectrum of fHLH genes in East Asian and Korean populations differed greatly from those of other ethnic groups.
Clinical application of artificial intelligence in longitudinal image analysis of bone age among GHD patients
Authors: Zhang Lina , Chen Jia , Hou Lele , Xu Yingying , Liu Zulin , Huang Siqi , Ou Hui , Meng Zhe , Liang Liyang
Published: November 2022

Objective

This study aims to explore the clinical value of artificial intelligence (AI)-assisted bone age assessment (BAA) among children with growth hormone deficiency (GHD).

Methods

A total of 290 bone age (BA) radiographs were collected from 52 children who participated in the study at Sun Yat-sen Memorial Hospital between January 2016 and August 2017. Senior pediatric endocrinologists independently evaluated BA according to the China 05 (CH05) method, and their consistent results were regarded as the gold standard (GS). Meanwhile, two junior pediatric endocrinologists were asked to assessed BA both with and without assistance from the AI-based BA evaluation system. Six months later, around 20% of the images assessed by the junior pediatric endocrinologists were randomly selected to be re-evaluated with the same procedure half a year later. Root mean square error (RMSE), mean absolute error (MAE), accuracy, and Bland-Altman plots were used to compare differences in BA. The intra-class correlation coefficient (ICC) and one-way repeated ANOVA were used to assess inter- and intra-observer variabilities in BAA. A boxplot of BA evaluated by different raters during the course of treatment and a mixed linear model were used to illustrate inter-rater effect over time.

Results

A total of 52 children with GHD were included, with mean chronological age and BA by GS of 6.64 ± 2.49 and 5.85 ± 2.30 years at baseline, respectively. After incorporating AI assistance, the performance of the junior pediatric endocrinologists improved (P < 0.001), with MAE and RMSE both decreased by more than 1.65 years (Rater 1: ΔMAE = 1.780, ΔRMSE = 1.655; Rater 2: ΔMAE = 1.794, ΔRMSE = 1.719), and accuracy increasing from approximately 10% to over 91%. The ICC also increased from 0.951 to 0.990. During GHD treatment (at baseline, 6-, 12-, 18-, and 24-months), the difference decreased sharply when AI was applied. Furthermore, a significant inter-rater effect (P = 0.002) also vanished upon AI involvement.

Conclusion

AI-assisted interpretation of BA can improve accuracy and decrease variability in results among junior pediatric endocrinologists in longitudinal cohort studies, which shows potential for further clinical application.
Common recognition topology of mex transporters of Pseudomonas aeruginosa revealed by molecular modelling
Authors: Catte Andrea , K. Ramaswamy Venkata , Vargiu Attilio Vittorio , Malloci Giuliano , Bosin Andrea , Ruggerone Paolo
Published: November 2022

The secondary transporters of the resistance-nodulation-cell division (RND) superfamily mediate multidrug resistance in Gram-negative bacteria like Pseudomonas aeruginosa. Among these RND transporters, MexB, MexF, and MexY, with partly overlapping specificities, have been implicated in pathogenicity. Only the structure of the former has been resolved experimentally, which together with the lack of data about the functional dynamics of the full set of transporters, limited a systematic investigation of the molecular determinants defining their peculiar and shared features. In a previous work (Ramaswamy et al., Front. Microbiol., 2018, 9, 1144), we compared at an atomistic level the two main putative recognition sites (named access and deep binding pockets) of MexB and MexY. In this work, we expand the comparison by performing extended molecular dynamics (MD) simulations of these transporters and the pathologically relevant transporter MexF. We employed a more realistic model of the inner phospholipid membrane of P. aeruginosa and more accurate force-fields. To elucidate structure/dynamics-activity relationships we performed physico-chemical analyses and mapped the binding propensities of several organic probes on all transporters. Our data revealed the presence, also in MexF, of a few multifunctional sites at locations equivalent to the access and deep binding pockets detected in MexB. Furthermore, we report for the first time about the multidrug binding abilities of two out of five gates of the channels deputed to peripheral (early) recognition of substrates. Overall, our findings help to define a common “recognition topology” characterizing Mex transporters, which can be exploited to optimize transport and inhibition propensities of antimicrobial compounds.
Acute hepatitis of unknown origin in children: A combination of factors
Authors: Gong Kai , Xu Xianbin , Yao Junjie , Ye Shaoheng , Yu Xia , Tu Huilan , Lan Yan , Fan Yu-chen , Shi Yu
Published: November 2022

On 5 April 2022, the World Health Organization was notified of 10 cases of severe acute hepatitis of unknown etiology in children under 10 years of age in the United Kingdom. Although the exact cause of a proportion of pediatric acute hepatitis and acute liver failure cases was unclear, the above event has caused widespread concern worldwide. As of 14 September 2022, approximately 1,296 probable cases of acute hepatitis of unknown etiology have been reported from 37 countries/regions, of which approximately 55 required or received liver transplantation and 29 died. Although the etiology of acute hepatitis of unknown origin in children remains unclear, many hypotheses have been proposed about the disease. Instead of individual factors such as “adenovirus infection,” “SARS-CoV-2 related,” and “Adeno-associated virus 2 with helper virus coinfection,” it is more likely due to a combination of factors. Accordingly, there is an urgent need for more data and research to clarify the disease etiology. This review aims to provide a historical perspective of acute hepatitis of unknown etiology in children in the past decades and summarize the current hypothesis and evidence on this emerging disease.
Imbalance hepatic metabolism homeostasis in the F1 generation of endometrial DNMT3B conditional knockout female mice
Authors: Li Weike , Gao Rufei , Ding Yubin , Chen Xuemei , Liu Xueqing , He Junlin , Li Fangfang , Long Jing , Lu Siyu , Yang Chengshun , Wang Yingxiong
Published: November 2022

Numerous studies have suggested the possibility of explaining the etiology of metabolic syndrome through DNA methylation. DNA methyltransferase 3B (DNMT3B) plays an important role in de novo DNA methylation. There was an alteration in maternal (F0) endometrial function, which might lead to growth and developmental disorder in offspring (F1). In this study, we investigated the effect of maternal endometrial DNMT3B deficiency on the metabolism in offspring. We constructed endometrial DNMT3B conditional knockout female mice (cKO) which were mated with normal C57BL/6 male mice to obtain the F1 generation. Further, to study the development of these offspring, we observed them at three different life stages which included the 6-week-old juvenile, 9-week-old sub-adult and 12-week-old adult. Follow the detection of a range of metabolism-related indicators, we found that in the cKO F1 generation, liver triglyceride level was significantly elevated in 9-week-old female mice, lipid droplet deposition was significantly increased in 9-week-old and 12-week-old mice, and the expression of lipid metabolism key factors in the liver was markedly decreased except of 6-week-old male mice. These results indicate that maternal endometrial DNMT3B conditional knockout leads to imbalance in hepatic metabolism in F1 generation, the mechanism of which requires further discussion.
Arbuscular mycorrhizal fungi in the soil using cover crops with and without nitrogen addition
Authors: Moura Jadson Belem de , Ramos Maria Lucrécia Gerosa , Konrad Maria Luiza de Freitas , Saggin Júnior Orivaldo José , Ribeiro Junior Walter Quadros , Carvalho Arminda Moreira de , Santos Luana de Miranda , Souza Rodrigo Fernandes de , Lopes Filho Luiz César , Dutra e Silva Sandro
Published: November 2022

This study aimed to evaluate the occurrence of mycorrhizal fungi and glomalin content in soil under different cover crops with and without the application of nitrogen in the cover. The following cover plants were used: Crotalaria juncea (Crotalaria junceaL.), wild beans from Ceará (Canavalia brasiliensisMart. ex Benth.), Guandú ‘BRS mandarin’ [Cajanus cajan(L.) Millsp.], millet ‘BR05’ [Pennisetum glaucum(L.) R.Br.] and sorghum ‘BR 304’ [Sorghum bicolor(L.) Moench]. The absolute control of the experiment was the treatment without the use of cover crops, that is, the vegetation of spontaneous occurrence in the area. The experimental design was randomized blocks in subplots with three replications. Spore density, mycorrhizal colonization rate, easily extractable glomalin, and species present in the rhizosphere of the cover crops were determined. No differences were found in the diversity of mycorrhizal fungi associated with the different cover crops studied or in the values of spore density, root colonization, or glomalin content. Nitrogen application did not influence the mycorrhizal activity in the investigated cover crops. The most frequent species associated with cover crops wereScutellospora pellucidaandScutellospora persicainC. juncea;Gigasporasp. on Sorghum;Glomus macrocarpumin Guandu;G. macrocarpumandGlomus clavisporumin millet; andGlomus microaggregatumandGlomus tortuosumin Spontaneous Vegetation.
Genetic background- and environment-independent QTL and candidate gene identification of appearance quality in three MAGIC populations of rice
Authors: Chen Huizhen , Zhai Laiyuan , Chen Kai , Shen Congcong , Zhu Shuangbing , Qu Pingping , Tang Jie , Liu Jianping , He Haohua , Xu Jianlong
Published: November 2022

Many QTL have been identified for grain appearance quality by linkage analysis (LA) in bi-parental mapping populations and by genome-wide association study (GWAS) in natural populations in rice. However, few of the well characterized genes/QTL have been successfully applied in molecular rice breeding due to genetic background (GB) and environment effects on QTL expression and deficiency of favorable alleles. In this study, GWAS and LA were performed to identify QTL for five grain appearance quality-related traits using three multi-parent advanced generation inter-cross (MAGIC) populations. A total of 22 QTL on chromosomes 1-3, 5-8 were identified by GWAS for five traits in DC1, DC2 and 8way, and four combined populations DC12 (DC1+DC2), DC18 (DC1+8way), DC28 (DC2+8way) and DC128 (DC1+DC2+8way). And a total of 42 QTL were identified on all 12 chromosomes except 10 by LA in the three single populations. Among 20 QTL identified by GWAS in DC1, DC2 and 8way, 10, four and three QTL were commonly detected in DC18, DC28, and DC128, respectively. Similarly, among 42 QTL detected by LA in the three populations, four, one and two QTL were commonly detected in DC18, DC28, and DC128, respectively. There was no QTL mapped together in DC12 by both two mapping methods, indicating that GB could greatly affect the mapping results, and it was easier to map the common QTL among populations with similar GB. The 8way population was more powerful for QTL mapping than the DC1, DC2 and various combined populations. Compared with GWAS, LA can not only identify large-effect QTL, but also identify minor-effect ones. Among 11 QTL simultaneously detected by the two methods in different GBs and environments, eight QTL corresponded to known genes, including AqGL3b and AqGLWR3a for GL and GLWR, AqGW5a, AqGLWR5, AqDEC5 and AqPGWC5 for GW, GLWR, DEC and PGWC, and AqDEC6b and AqPGWC6b for DEC and PGWC, respectively. AqGL7, AqGL3c/AqGLWR3b, AqDEC6a/AqPGWC6a, and AqPGWC7 were newly identified and their candidate genes were analyzed and inferred. It was discussed to further improve grain appearance quality through designed QTL pyramiding strategy based on the stable QTL identified in the MAGIC populations.
Development of the maize 5.5K loci panel for genomic prediction through genotyping by target sequencing
Authors: Ma Juan , Cao Yanyong , Wang Yanzhao , Ding Yong
Published: November 2022

Genotyping platforms are important for genetic research and molecular breeding. In this study, a low-density genotyping platform containing 5.5K SNP markers was successfully developed in maize using genotyping by target sequencing (GBTS) technology with capture-in-solution. Two maize populations (Pop1 and Pop2) were used to validate the GBTS panel for genetic and molecular breeding studies. Pop1 comprised 942 hybrids derived from 250 inbred lines and four testers, and Pop2 contained 540 hybrids which were generated from 123 new-developed inbred lines and eight testers. The genetic analyses showed that the average polymorphic information content and genetic diversity values ranged from 0.27 to 0.38 in both populations using all filtered genotyping data. The mean missing rate was 1.23% across populations. The Structure and UPGMA tree analyses revealed similar genetic divergences (76-89%) in both populations. Genomic prediction analyses showed that the prediction accuracy of reproducing kernel Hilbert space (RKHS) was slightly lower than that of genomic best linear unbiased prediction (GBLUP) and three Bayesian methods for general combining ability of grain yield per plant and three yield-related traits in both populations, whereas RKHS with additive effects showed superior advantages over the other four methods in Pop1. In Pop1, the GBLUP and three Bayesian methods with additive-dominance model improved the prediction accuracies by 4.89-134.52% for the four traits in comparison to the additive model. In Pop2, the inclusion of dominance did not improve the accuracy in most cases. In general, low accuracies (0.33-0.43) were achieved for general combing ability of the four traits in Pop1, whereas moderate-to-high accuracies (0.52-0.65) were observed in Pop2. For hybrid performance prediction, the accuracies were moderate to high (0.51-0.75) for the four traits in both populations using the additive-dominance model. This study suggests a reliable genotyping platform that can be implemented in genomic selection-assisted breeding to accelerate maize new cultivar development and improvement.
The effect of the perceived social support on mental health of Chinese college soccer players during the COVID-19 lockdown: The chain mediating role of athlete burnout and hopelessness
Authors: Zhao Liangyu , Liu Zongyu , Zhang Liguo
Published: November 2022

The isolation and blockade during the COVID-19 pandemic have a great impact on the mental health of Chinese college soccer players. This study aimed to explore the effect of perceived social support on mental health of college soccer players, as well as the mediating role of athlete burnout and hopelessness during the COVID-19 lockdown. Based on a sample of 674 college soccer players from several universities in China, the study used the Perceived Social Support Scale (PSSS), Kessler Psychological Distress Scale (K10), Athlete Burnout Questionnaire (ABQ) and Beck Hopelessness Scale (BHS). The results indicated that: (1) there was a significant positive correlation between perceived social support and mental health of college soccer players; (2) the athlete burnout played a mediating role between perceived social support and mental health of college soccer players; (3) the hopelessness also played a mediating role between perceived social support and mental health of college soccer players; (4) the athlete burnout and hopelessness played a chain mediating role between perceived social support and mental health. These findings could contribute to insight into the mechanisms by which perceived social support affects the mental health of college soccer players and provide important practical guidance for protecting and promoting their mental health.
Dialect diversity and total factor productivity: Evidence from Chinese listed companies
Authors: Xiong Jiacai , Chen Linghong
Published: November 2022

Using a sample of Chinese listed companies over the 2007–2019 period, we examined the influence of dialect diversity on a firm's total factor productivity. We found that dialect diversity affects the psychological distance of interpersonal communication and significantly affects the firm's total factor productivity. The results are robust to a battery of tests based on different specifications. The relationship between dialect diversity and a firm's total factor productivity is more pronounced in state-owned enterprises, firms located in southern regions, and more capital-intensive firms. Furthermore, we demonstrated an innovative factor flow mechanism and a human capital accumulation mechanism through which dialect diversity inhibits total factor productivity. Overall, this paper provides new evidence and decision-making reference for coordinating the protection of dialect diversity and high-quality economic development.
Research on the impact of live streaming marketing by online influencers on consumer purchasing intentions
Authors: Wang Xueli , Aisihaer Nadilai , Aihemaiti Aihetanmujiang
Published: November 2022

Drawing from the stimulus–organism–response (S-O-R) model, this study explores the impact on consumer attitudes in the context of Chinese online influencers' e-commerce live streaming. To examine this impact, we distributed our survey questionnaire to Chinese consumers with live streaming shopping experiences. Using data from 430 valid questionnaires, a hierarchical regression analysis was used to examine our hypotheses. The results show that expertise, bargaining power, post-sales services, and live streaming schedules of online influencers affect consumer trust in online influencers. The expertise, bargaining power, and livestreaming schedules of online influencers affect consumer impulsivity. Moreover, the trust and impulsiveness of online influencers increase consumer purchasing intentions. The implications and future research directions are discussed in this article.
A survey of health status of healthcare providers in a square cabin hospital during the new corona omicron outbreak: A cross-sectional study
Authors: Pei Haoyu , Gan Xiaoqin , Guo Fei , Wu Qiuping , Liu Ding , Li Zhouzhou , Lan Ping , Zhang Lili , Yan Hong
Published: November 2022

Background

The coronavirus omicron variant outbroke in early 2022 in Shanghai. Although previous studies indicated that long working hours in a square cabin hospital might increase the risk of mental health among frontline healthcare providers, few studies have investigated whether the mental health risk could be reduced among well-trained professionals following the new guidelines.

Objective

This study aimed to investigate the health situation of frontline healthcare providers in Shanghai square cabin during the omicron variant circulation.

Methods

An online survey was used to evaluate those healthcare providers working in the square cabin hospitals from March 1, 2022, to May 31, 2022. The first online survey was conducted and emailed to the health providers on April 1. The second survey was conducted and sent to the nonrespondents on May 31. Overall, 142 frontline healthcare providers completed the online survey. Their mental health was assessed by the Insomnia Severity Index Scale, the Generalized Anxiety Disorder Scale, the Patient Health Questionnaire-9, and the Psychological Resilience Scale. We estimated multiple clinical systems and identified factors associated with those symptoms among participants. Multivariable logistic regression models were used to assess the risk factors of these symptoms.

Results

Overall, 66.20%, 45.07%, and 27.46% of frontline healthcare providers in Shanghai City reported symptoms of insomnia, depression, and anxiety, respectively. In addition, the most common symptoms included dry eyes (57.75%), lumbar muscle strain (47.18%), dry mouth (35.92%), itching (31.69%), headache (29.58%), and sore throat (28.87%) among the frontline healthcare providers. There was no statistical difference in symptoms by gender, age, personnel category, or job position (p > 0.05).

Conclusion

In the case of an unexpected pandemic, the mental health of healthcare providers is not optimistic. This situation still exists more than 2 years after the global outbreak of the COVID-19 pandemic. Therefore, the physical and mental health of long-term healthcare providers working in a square cabin hospital still needs monitoring.
The Swedish version of the multidimensional inventory for religious/spiritual well-being – Part II: Development of a four-field typology
Authors: Podolin-Danner Nikita , Wenzl Magdalena , Knorr Anna , Fuchshuber Jürgen , Silani Giorgia , Unterrainer Human-Friedrich
Published: November 2022

Background

In the field of mental health, religiosity and spirituality have gained particular attention in recent decades. However, only a few studies to date have investigated the effects of different types of religiosity and spirituality. In association with the recent introduction of a Swedish version of the multidimensional inventory of religious/spiritual well-being (MI-RSWB-S), the present study aimed to identify possible types of Religious/Spiritual Well-Being by using cluster analyses and to examine the extracted groups for differences in the sense of coherence (SOC), the Big Five personality factors, and central aspects of religiosity. Additionally, the study design was intended to further contribute to the validation of the MI-RSWB-S.

Methods

Based on a convenience sample of Swedish students (N = 1,011), initially obtained for the development of the MI-RSWB-S, the study included the MI-RSWB-S, the 13-items sense of coherence scale, the 10-item personality inventory, and the centrality of religiosity scale. For the statistical analysis, cluster analyses and one-way analyses of variance (ANOVAs) were conducted.

Results

The cluster analyses yielded the following four groups: Religiosity and spirituality high (n = 124), religiously oriented (n = 200), spiritually oriented (n = 149), and religiosity and spirituality low (n = 538). The groups differed in most aspects of well-being, in the personality dimensions agreeableness and openness to experience, as well as in central aspects of religiosity. In contrast, no differences were found for SOC, extraversion, conscientiousness, and emotional instability.

Conclusion

Our results suggest that different types of religious/spiritual well-being are associated with mental health and personality dimensions in substantially different ways, thus offering an interesting potential for future research.
The impact of COVID-19 on the employment status and psychological expectations of college graduates: Empirical evidence from the survey data of Chinese recruitment websites
Authors: Mao Yufei , Zhang Yuan , Bai Jiaxin , Zhang Liangbo , Hu Wenxin
Published: November 2022

Based on the big data and survey data of online recruitment platform, this paper empirically tests the impact of COVID-19 on the employment status and psychological expectations of college graduates. The results show that: under the impact of COVID-19 epidemic, both supply and demand sides of college graduates’ employment market are affected, such as the decline of recruitment demand, the rise of the employment supply, and the obvious decrease of employment market prosperity. The impacts of COVID-19 epidemic on college graduates’ employment status and psychological expectation in different cities are heterogeneous. In the short term, the epidemic has a negative impact on the employment of graduates, but the employment situation is gradually improving with the support of national policies. Under the influence of COVID-19 epidemic, graduates will change their employment location and expected salary, and they tend to choose “temporary non-employment,” and their proportions of getting offers and signing contracts are significantly reduced. This paper suggests: Firstly, we should continue to push forward the action plan of “expanding jobs in graduation season to promote employment,” and strengthen the persistence and permanence of employment promotion policies for college graduates; Secondly, encourage college students to change their employment concept and rationally adjust their employment expectations; Thirdly, to promote the development of flexible employment of college graduates, it is necessary to strengthen the propaganda of flexible employment, so that students can understand relevant policies; Fourthly, strengthen employment guidance services for graduates from poor families to ensure the continuity and stability of employment assistance policies.
Alexithymia and post-traumatic stress disorder symptoms in Chinese undergraduate students during the COVID-19 national lockdown: The mediating role of sleep problems and the moderating role of self-esteem
Authors: Zhang Yi , Zhao Yijin , Ni Ting , Chen Jing , Tang Wanjie
Published: November 2022

Objective

This study examined whether sleep disturbance was a mediator between alexithymic traits and post-traumatic stress disorder (PTSD) COVID-19 pandemic-related stress symptoms, and explored whether self-esteem moderated the alexithymic contribution to poor sleep and PTSD symptoms.

Method

A representative sample of young adults (N = 2,485) from six universities in Southwest China completed online self-report surveys on alexithymia, sleep, PTSD, self-esteem, sociodemographic information, and health-related behaviors.

Results

High alexithymic young adults were found to be more likely to have higher sleep problems and higher PTSD symptoms. The moderated mediation model showed that sleep problems mediated the associations between alexithymia and PTSD symptoms. Alexithymic people with lower self-esteem were more likely to have elevated PTSD symptoms and sleep problems than those with higher self-esteem.

Conclusion

Targeted psychological interventions for young people who have difficulty expressing and identifying emotions are recommended as these could assist in reducing their post-traumatic psychophysical and psychological problems. Improving self-esteem could also offer some protection for trauma-exposed individuals.
Being helpful and being innovative: The role of psychological meaningfulness and positive affect
Authors: Shen Yifei , Zhang Zhenduo , Song Haoyang , Zheng Junwei , Bu Qiong
Published: November 2022

The present study is developed based on conservation of resources theory (COR) to explore the underlying mechanism and boundary condition for the relationship between helping behavior and innovative behavior. To avoid the shortages of cross-sectional data, the present study collected two-wave and multi-source data. By collecting from 193 full-time Chinese workers and 68 supervisors at two separate time points, this study developed and examined a moderated mediation model using Mplus 7.0. The results show that helping behavior increases innovative behavior through enhancing positive affect, and psychological meaningfulness moderates the indirect relationship between helping behavior and innovative behavior through positive affect. In the condition of high psychological meaningfulness, helping behavior has a stronger indirect impact on innovative behavior through enhancing positive affect. This study enriches the literature on the outcomes of helping behavior. Moreover, this study provides several managerial implications to amplify the positive impact of helping behavior on innovative behavior. This study develops several strategies to enhance psychological meaningfulness and promote the benefits of helping behavior.