bluuebunny/crossref_metadata_2025 huggingface dataset:
Rhodopsin 5– and Rhodopsin 6–Mediated Clock Synchronization in Drosophila melanogaster Is Independent of Retinal Phospholipase C-β Signaling
Authors: Szular Joanna , Sehadova Hana , Gentile Carla , Szabo Gisela , Chou Wen-Hai , Britt Steven G. , Stanewsky Ralf
Published: February 2012

Circadian clocks of most organisms are synchronized with the 24-hour solar day by the changes of light and dark. In Drosophila, both the visual photoreceptors in the compound eyes as well as the blue-light photoreceptor Cryptochrome expressed within the brain clock neurons contribute to this clock synchronization. A specialized photoreceptive structure located between the retina and the optic lobes, the Hofbauer-Buchner (H-B) eyelet, projects to the clock neurons in the brain and also participates in light synchronization. The compound eye photoreceptors and the H-B eyelet contain Rhodopsin photopigments, which activate the canonical invertebrate phototransduction cascade after being excited by light. We show here that 2 of the photopigments present in these photoreceptors, Rhodopsin 5 (Rh5) and Rhodopsin 6 (Rh6), contribute to light synchronization in a mutant ( norpAP41) that disrupts canonical phototransduction due to the absence of Phospholipase C-β (PLC-β). We reveal that norpAP41 is a true loss-of-function allele, resulting in a truncated PLC-β protein that lacks the catalytic domain. Light reception mediated by Rh5 and Rh6 must therefore utilize either a different (nonretinal) PLC-β enzyme or alternative signaling mechanisms, at least in terms of clock-relevant photoreception. This novel signaling mode may distinguish Rhodopsin-mediated irradiance detection from image-forming vision in Drosophila.
Association of limited joint mobility and increased plantar hardness in diabetic foot ulceration in north Asian Indian: A preliminary study
Authors: Periyasamy R , Anand Sneh , Ammini AC
Published: February 2012

The aim of this article is to investigate the association of limited joint mobility and foot sole hardness in north Asian Indian type 2 diabetic patients. Limited joint mobility and hardness of the foot sole were measured for 39 subjects attending the AIIMS Endocrinology & Metabolism Clinic. The total subject divided into three groups: 13 control subjects (non-diabetic), 13 diabetic patients without neuropathy and 13 diabetic neuropathy patients. Neuropathy status was assessed using 10 gm Semen’s Weinstein monofilament. Joint mobility parameters, such as ankle dorsiflexion/plantar flexion and metatarsophalangeal–I dorsiflexion/plantar flexion, are measured using a goniometer. Foot sole hardness was measured using a durometer or shore meter. We found that diabetic patients with a neuropathic foot had significantly reduced joint mobility and increased foot sole hardness, placing them at risk for subsequent ulceration. Metatarsophalangeal-I dorsiflexion/plantar flexion of both feet of diabetic patients had significant correlation (at p < 0.05, p < 0.001, p < 0.001 level) over age and body mass index. Also ankle plantar flexion/dorsiflexion and metatarsophalangeal-I dorsiflexion/plantar flexion has a significant correlations (at p < 0.01, p < 0.05, p < 0.001, p < 0.001 level) with foot sole hardness in both feet of diabetic neuropathy subjects. Also linear regression analysis showed that duration of diabetes was significantly associated with the joint mobility parameters. In this study we conclude that joint mobility had reduced further if neuropathy and increased foot sole hardness coexisted owing to high plantar pressures. Hence, both limited joint mobility and increased foot sole hardness appears to be important determinants of foot sole ulceration in diabetic neuropathic subject.
Travels, Songs and Displacements
Authors: Mukherjee Rahul
Published: February 2012

This article analyzes a set of translocal documentary films by always-on-the-move filmmakers who attempt to connect place-based struggles against exploitative development projects. These films stitch together recorded footage from various locales/places to bring out the commonalities and shared patterns among them, thereby creating translocal/transplace alliances interrogating the dominant development discourse. In some of these works, train journeys between two places become a way of connecting to the past and renewing erstwhile translocal ties. I examine the links between aesthetic strategies of depicting movement in the documentaries, and their political critique of the displacement and forced migration of indigenous communities in the wake of exogenous developmental interventions. While each documentary film highlights “movement,” the formal elements that achieve this vary from songs narrating journeys to expressive montages with multilayered soundtracks. Engaging in a discussion of Kora Rajee, which remembers and redeploys indigenous historical experiences of travel of the Oraon tribe, I explore how critiques of development can be approached by attending to the changing cultural-politics of tribal identities and their representation through documentaries.
Grief and Work
Authors: Han Yuri
Published: February 2012

A seemingly rational workplace, in fact, is full of intertwined emotional events, and grief is one of them. However, the significance of underlying emotions that employees carry with them to the workplace and the impact of employees’ perceived notions toward the organization about the way management handles employee problems are not recognized and properly understood. I learned about my own emotional struggles and those of my coworkers while experiencing the loss of a close colleague, Jay, with cancer. This autoethnographical study is my attempt to formulate a narrative of the whole experience to elicit subjective meanings and learning from it.
Impact of pregnancy on conversion to clinically isolated syndrome in a radiologically isolated syndrome cohort
Authors: Lebrun C , Le Page E , Kantarci O , Siva A , Pelletier D , Okuda DT ,   on behalf of the Club Francophone de la Sclerose en Plaques (CFSEP) and the Radiologically Isolated Syndrome Consortium (RISC) Group
Published: February 2012

Background:

In multiple sclerosis (MS), the relapse rate declines during pregnancy and increases during the first three months post-partum before returning to the pre-pregnancy rate. It is unknown whether pregnancy impacts the risk of clinical conversion in those within the presymptomatic period.

Objectives:

We investigate the impact of pregnancy on developing a clinical event in women diagnosed with radiologically isolated syndrome (RIS).

Methods:

All women with RIS underwent clinical and radiological assessments as part of an observational, prospective, longitudinal study. Clinical and MRI outcomes were analyzed during and after pregnancy. Subjects who became pregnant were compared with an age-matched female RIS group who did not become pregnant during the same follow-up period.

Results:

A total of 60 women with RIS were followed for up to seven years. Among them, seven became pregnant and were compared with 53 age-matched control women with RIS who did not become pregnant during the observation period. A significantly shorter time of conversion to the first neurological event was observed in the pregnant group [15.3 months (10–18)] compared with the non-pregnant controls [35.7 months (8–76)], yielding an absolute difference of 20.4 months ( p<0.05). The mean (SD) number of active lesions on a subsequent brain MRI scan was significantly higher in the pregnant group [3.2 (±1.7)] compared with the control group [1.8 (±0.6)].

Conclusions:

The risk for clinical conversion from RIS to a clinical event and new MRI disease activity seems to be influenced by pregnancy. Pregnancy related physiological changes could operate as early as the presymptomatic period in patients with MS.
Cool places, creative places? Community perceptions of cultural vitality in the suburbs
Authors: Gibson Chris , Brennan-Horley Chris , Laurenson Beth , Riggs Naomi , Warren Andrew , Gallan Ben , Brown Heidi
Published: February 2012

This article stems from a project examining cultural assets in Wollongong – a medium-sized Australian city with a decentralized and linear suburban pattern that challenges orthodox binaries of inner-city bohemia/outer-suburban domesticity. In Wollongong we documented community perceptions of cultural assets across this unusual setting, through a simple public research method. At the city’s largest annual festival we recruited the general public to nominate the city’s most ‘cool’ and ‘creative’ places, by drawing on a map of Wollongong and telling their stories. Hand-drawn maps from 205 participants were combined in a Geographical Information System and 50 hours of stories transcribed for qualitative analysis. Over 2300 places were identified. Among them were some surprising results: although places known for the arts and bohemian creative industries figured prominently, these were not only in the inner-city but in beachside suburbs with unique cultural histories. Also, a range of affective engagements with place, including unconventional forms of creativity, were described in industrial and blue-collar suburbs. Network topology analysis by place of residence also revealed the extent of localism, as well as specializations and aggrandizements among suburbs. Our conclusions are threefold: first, that ‘creativity’ is relationally situated and linked across all parts of the city; second, that decentralized forms of small-scale cultural infrastructure provision are vital for vernacular cultural pursuits; and, third, that ‘creativity’ is a polysemic and contested category – only ever partially revealing the contours of cultural vitality in the suburbs.
Australian television, popular memory and suburbia
Authors: McKee Alan
Published: February 2012

Different archives of television material construct different versions of Australian national identity. There exists a Pro-Am archive of Australian television history materials consisting of many individual collections. This archive is not centrally located nor clearly bounded. The collections are not all linked to each other, nor are they aware of each other, and they do not claim to have a single common project. Pro-Am collections tend not to address Australian television as a whole, rather addressing particular genres, programs or production companies. Their vision of Australia is ‘ordinary’ and everyday. The boundaries of ‘Australia’ in the Pro-Am archive are porous, allowing non-Australians to contribute material, and also including non-Australian material and this causes little sense of anxiety.
The impact of lung cancer on patients and carers
Authors: Ellis Jackie
Published: February 2012

This article aims to provide a brief review of the literature with regard to the impact of lung cancer on patients and their informal carers. Compared to other types of cancer, the distress associated with lung cancer has been found to be the most intense. Rather than focusing on symptoms in isolation recent emphasis regarding the symptom experience has been on symptoms clusters, as understanding these clusters may improve the management of ongoing and unrelieved symptoms. However, the disparities in methodology are significant barriers to producing comparable results, although recent efforts have been made to address these. Whilst research into symptoms has enormous potential for the management of symptom clusters, it needs to move away from the essentially reductionist stance which currently dominates and broaden its scope to one that acknowledges the complexity of the experience of symptom clusters from the perspective of the patient and their informal carer. Poor management of symptoms complicates patient care and potentially contributes to the heavy burden which often falls on family caregivers, especially as the disease progresses. The majority of studies focus on the experiences of primary care providers, most often the partner/spouse. Such studies have shown that spouses of patients with lung cancer exhibit significant distress and lower levels of quality of life than the general population. Research also indicates that significant others go through a transition process due to changes brought about by the diagnosis of lung cancer and struggle to endure and overcome difficulties and distress. Significant others were seen to suffer during this process of transition and experienced altered relationships. Clinicians working with patients suffering from lung cancer and their carers should intervene to enhance their quality of life from diagnosis, during the disease trajectory and during bereavement. Interventions need to be developed to support both patients and carers.
Participatory Culture and the New Governance of Communication
Authors: Langlois Ganaele
Published: February 2012

This article develops a critical alternative to the common equation between participatory culture and democratic communication and argues that power on online participatory platforms should be understood as the governance of semiotic open-endedness. This article argues that the concept of cultural expression cannot be understood solely by looking at users’ cultural practices, but should be revisited to pay attention to the networked conditions that enable it. This involves tracing the governance of disparate processes such as protocols, software, linguistic processes, and cultural practices that make the production and circulation of meaning possible. Thus, communication on participatory platforms should be understood as the management of flows of meaning, that is, as the processes of codification of the informational, technical, cultural, and semiotic dynamics through which meanings are expressed. This makes it possible to understand the logics through which software platforms transform information into cultural signs and shape users’ perceptions and agencies.
Early Detection of Metabolic Changes Using Microdialysis During and After Experimental Kidney Transplantation in a Porcine Model
Authors: Fonouni Hamidreza , Esmaeilzadeh Majid , Jarahian Parvin , Rad Morva Tahmasbi , Golriz Mohammad , Faridar Alireza , Hafezi Mohammadreza , Jafarieh Shadi , Kashfi Arash , Yazdi Seid Hashem Fani , Soleimani Mehrdad , Longerich Thomas , Shevchenko Maxim , Sakowitz Oliver , Schmidt Jan , Mehrabi Arianeb
Published: February 2012

Background: Microdialysis (MD) can detect organ-related metabolic changes before they become measurable in plasma through the biochemical parameters. This study aims to evaluate the early detection of metabolic changes during experimental kidney transplantation (KTx). Material and methods: During preparation of 8 donor kidneys, one MD catheter was inserted in the renal cortex and samples were collected. After a 6-hour cold ischemia time (CIT), kidneys were implanted in the 8 recipient pigs. Throughout the warm ischemia time (WIT) and after reperfusion, kidneys were monitored. The interstitial glucose, lactate, pyruvate, glutamate, and glycerol concentrations were evaluated. Results: A significant decline in glucose level was observed at the end of CIT. The lactate level was reduced to the minimum point of 0.35 ± 0.08 mmol/L in CIT. After reperfusion, lactate values raised significantly. During the WIT, the pyruvate level increased, continued until the end of the WIT. For glutamate, a steady increase was noted during explantation, CIT, WIT, and early reperfusion phases. The increase of glycerol value continued in the early postreperfusion, which was then followed by a sharp decline. Conclusion: MD is a fast and simple minimally invasive method for measurement of metabolic substrates in renal parenchyma during KTx. MD offers the option of detecting minor changes of interstitial glucose, lactate, pyruvate, glutamate, and glycerol in every stage of KTx. Through the use of MD, metabolic changes can be continuously monitored during the entire procedure of KTx.
Glycoproteins as targets of autoantibodies in CNS inflammation: MOG and more
Authors: Mayer Marie Cathrin , Meinl Edgar
Published: February 2012

B cells and antibodies constitute an important element in different inflammatory diseases of the central nervous system (CNS). Autoantibodies can serve as a biomarker to identify disease subgroups and may in addition contribute to the pathogenic process. One candidate autoantigen for multiple sclerosis (MS) is myelin oligodendrocyte glycoprotein (MOG). MOG is localized at the outermost surface of myelin in the CNS and has been the focus of extensive research for more than 30 years. Its role as an important autoantigen for T cells and as a target of demyelinating autoantibodies has been established in several variants of experimental autoimmune encephalomyelitis (EAE), an animal model of MS. The literature regarding antibodies to MOG in MS patients is confusing and contradictory. Recent studies, however, have described high levels of antibodies to conformationally correct MOG in pediatric acquired demyelination, both acute disseminated encephalomyelitis (ADEM) and MS. In adult MS, such antibodies are rarely found and then only at low levels. In this review, we summarize key findings from animal models and patient studies, discuss challenges in detecting anti-MOG antibodies in patients and present recent approaches to identifying new autoantigens in MS.
A phase 1/2 study of chemosensitization with the CXCR4 antagonist plerixafor in relapsed or refractory acute myeloid leukemia
Authors: Uy Geoffrey L. , Rettig Michael P. , Motabi Ibraheem H. , McFarland Kyle , Trinkaus Kathryn M. , Hladnik Lindsay M. , Kulkarni Shashikant , Abboud Camille N. , Cashen Amanda F. , Stockerl-Goldstein Keith E. , Vij Ravi , Westervelt Peter , DiPersio John F.
Published: February 2012

The interaction of acute myeloid leukemia (AML) blasts with the leukemic microenvironment is postulated to be an important mediator of resistance to chemotherapy and disease relapse. We hypothesized that inhibition of the CXCR4/CXCL12 axis by the small molecule inhibitor, plerixafor, would disrupt the interaction of leukemic blasts with the environment and increase the sensitivity of AML blasts to chemotherapy. In this phase 1/2 study, 52 patients with relapsed or refractory AML were treated with plerixafor in combination with mitoxantrone, etoposide, and cytarabine. In phase 1, plerixafor was escalated to a maximum of 0.24 mg/kg/d without any dose-limiting toxicities. In phase 2, 46 patients were treated with plerixafor 0.24 mg/kg/d in combination with chemotherapy with an overall complete remission and complete remission with incomplete blood count recovery rate (CR + CRi) of 46%. Correlative studies demonstrated a 2-fold mobilization in leukemic blasts into the peripheral circulation. No evidence of symptomatic hyperleukocytosis or delayed count recovery was observed with the addition of plerixafor. We conclude that the addition of plerixafor to cytotoxic chemotherapy is feasible in AML, and results in encouraging rates of remission with correlative studies demonstrating in vivo evidence of disruption of the CXCR4/CXCL12 axis. This study was registered at www.clinicaltrials.gov, no. NCT00512252.
HIF-1α deletion partially rescues defects of hematopoietic stem cell quiescence caused by Cited2 deficiency
Authors: Du Jinwei , Chen Yu , Li Qiang , Han Xiangzi , Cheng Cindy , Wang Zhengqi , Danielpour David , Dunwoodie Sally L. , Bunting Kevin D. , Yang Yu-Chung
Published: February 2012

Cited2 is a transcriptional modulator involved in various biologic processes including fetal liver hematopoiesis. In the present study, the function of Cited2 in adult hematopoiesis was investigated in conditional knockout mice. Deletion of Cited2 using Mx1-Cre resulted in increased hematopoietic stem cell (HSC) apoptosis, loss of quiescence, and increased cycling, leading to a severely impaired reconstitution capacity as assessed by 5-fluorouracil treatment and long-term transplantation. Transcriptional profiling revealed that multiple HSC quiescence- and hypoxia-related genes such as Egr1, p57, and Hes1 were affected in Cited2-deficient HSCs. Because Cited2 is a negative regulator of HIF-1, which is essential for maintaining HSC quiescence, and because we demonstrated previously that decreased HIF-1α gene dosage partially rescues both cardiac and lens defects caused by Cited2 deficiency, we generated Cited2 and HIF-1α double-knockout mice. Additional deletion of HIF-1α in Cited2-knockout BM partially rescued impaired HSC quiescence and reconstitution capacity. At the transcriptional level, deletion of HIF-1α restored expression of p57 and Hes1 but not Egr1 to normal levels. Our results suggest that Cited2 regulates HSC quiescence through both HIF-1–dependent and HIF-1–independent pathways.
Effects of nanoparticle zinc oxide on spatial cognition and synaptic plasticity in mice with depressive-like behaviors
Authors: Xie Yongling , Wang Yiyi , Zhang Tao , Ren Guogang , Yang Zhuo
Published: February 2012

Background

Nanomaterials, as a new kind of materials, have been greatly applied in different fields due to their special properties. With the industrialization of nanostructured materials and increasing public exposure, the biosafety and potential influences on central nervous system (CNS) have received more attention. Nanosized zinc oxide (nanoZnO) was suggested to up-regulate neuronal excitability and to induce glutamate release in vitro. Therefore, we hypothesized nanoparticles of nanoZnO may lead to changes in balance of neurotransmitter or neuronal excitability of CNS. This study was to investigate if there were effects of nanoZnO on animal model of depression.

Methods

Male Swiss mice were given lipopolysaccharides (LPS, 100 μg/kg, 100 μg/ml, every other day, 8 times, i.p.) from weaning to induce depressive-like behaviors. NanoZnO (5.6 mg/kg, 5.6 mg/ml, every other day, 8 times, i.p.) was given as the interaction. The mouse model was characterized using the methods of open field test, tail suspension test and forced swim test. Furthermore, the spatial memory was evaluated using Morris water maze (MWM) and the synaptic plasticity was assessed by measuring the long-term potentiation (LTP) in the perforant pathway (PP) to dentate gyrus (DG) in vivo.

Results

Results indicated that model mice showed disrupted spatial memory and LTP after LPS injections and the behavioral and electrophysiological improvements after nanoZnO treatment.

Conclusion

Data suggested that nanoZnO may play some roles in CNS of mental disorders, which could provide some useful direction on the new drug exploring and clinical researches.
Independent Principal Component Analysis for biologically meaningful dimension reduction of large biological data sets
Authors: Yao Fangzhou , Coquery Jeff , Lê Cao Kim-Anh
Published: February 2012

Background

A key question when analyzing high throughput data is whether the information provided by the measured biological entities (gene, metabolite expression for example) is related to the experimental conditions, or, rather, to some interfering signals, such as experimental bias or artefacts. Visualization tools are therefore useful to better understand the underlying structure of the data in a 'blind' (unsupervised) way. A well-established technique to do so is Principal Component Analysis (PCA). PCA is particularly powerful if the biological question is related to the highest variance. Independent Component Analysis (ICA) has been proposed as an alternative to PCA as it optimizes an independence condition to give more meaningful components. However, neither PCA nor ICA can overcome both the high dimensionality and noisy characteristics of biological data.

Results

We propose Independent Principal Component Analysis (IPCA) that combines the advantages of both PCA and ICA. It uses ICA as a denoising process of the loading vectors produced by PCA to better highlight the important biological entities and reveal insightful patterns in the data. The result is a better clustering of the biological samples on graphical representations. In addition, a sparse version is proposed that performs an internal variable selection to identify biologically relevant features (sIPCA).

Conclusions

On simulation studies and real data sets, we showed that IPCA offers a better visualization of the data than ICA and with a smaller number of components than PCA. Furthermore, a preliminary investigation of the list of genes selected with sIPCA demonstrate that the approach is well able to highlight relevant genes in the data with respect to the biological experiment.

IPCA and sIPCA are both implemented in the R package mixomics dedicated to the analysis and exploration of high dimensional biological data sets, and on mixomics' web-interface.
Post-genomic analyses of fungal lignocellulosic biomass degradation reveal the unexpected potential of the plant pathogen Ustilago maydis
Authors: Couturier Marie , Navarro David , Olivé Caroline , Chevret Didier , Haon Mireille , Favel Anne , Lesage-Meessen Laurence , Henrissat Bernard , Coutinho Pedro M , Berrin Jean-Guy
Published: February 2012

Background

Filamentous fungi are potent biomass degraders due to their ability to thrive in ligno(hemi)cellulose-rich environments. During the last decade, fungal genome sequencing initiatives have yielded abundant information on the genes that are putatively involved in lignocellulose degradation. At present, additional experimental studies are essential to provide insights into the fungal secreted enzymatic pools involved in lignocellulose degradation.

Results

In this study, we performed a wide analysis of 20 filamentous fungi for which genomic data are available to investigate their biomass-hydrolysis potential. A comparison of fungal genomes and secretomes using enzyme activity profiling revealed discrepancies in carbohydrate active enzymes (CAZymes) sets dedicated to plant cell wall. Investigation of the contribution made by each secretome to the saccharification of wheat straw demonstrated that most of them individually supplemented the industrial Trichoderma reesei CL847 enzymatic cocktail. Unexpectedly, the most striking effect was obtained with the phytopathogen Ustilago maydis that improved the release of total sugars by 57% and of glucose by 22%. Proteomic analyses of the best-performing secretomes indicated a specific enzymatic mechanism of U. maydis that is likely to involve oxido-reductases and hemicellulases.

Conclusion

This study provides insight into the lignocellulose-degradation mechanisms by filamentous fungi and allows for the identification of a number of enzymes that are potentially useful to further improve the industrial lignocellulose bioconversion process.
Analysis of the SOS response of Vibrio and other bacteria with multiple chromosomes
Authors: Sanchez-Alberola Neus , Campoy Susana , Barbé Jordi , Erill Ivan
Published: February 2012

Background

The SOS response is a well-known regulatory network present in most bacteria and aimed at addressing DNA damage. It has also been linked extensively to stress-induced mutagenesis, virulence and the emergence and dissemination of antibiotic resistance determinants. Recently, the SOS response has been shown to regulate the activity of integrases in the chromosomal superintegrons of the Vibrionaceae, which encompasses a wide range of pathogenic species harboring multiple chromosomes. Here we combine in silico and in vitro techniques to perform a comparative genomics analysis of the SOS regulon in the Vibrionaceae, and we extend the methodology to map this transcriptional network in other bacterial species harboring multiple chromosomes.

Results

Our analysis provides the first comprehensive description of the SOS response in a family (Vibrionaceae) that includes major human pathogens. It also identifies several previously unreported members of the SOS transcriptional network, including two proteins of unknown function. The analysis of the SOS response in other bacterial species with multiple chromosomes uncovers additional regulon members and reveals that there is a conserved core of SOS genes, and that specialized additions to this basic network take place in different phylogenetic groups. Our results also indicate that across all groups the main elements of the SOS response are always found in the large chromosome, whereas specialized additions are found in the smaller chromosomes and plasmids.

Conclusions

Our findings confirm that the SOS response of the Vibrionaceae is strongly linked with pathogenicity and dissemination of antibiotic resistance, and suggest that the characterization of the newly identified members of this regulon could provide key insights into the pathogenesis of Vibrio. The persistent location of key SOS genes in the large chromosome across several bacterial groups confirms that the SOS response plays an essential role in these organisms and sheds light into the mechanisms of evolution of global transcriptional networks involved in adaptability and rapid response to environmental changes, suggesting that small chromosomes may act as evolutionary test beds for the rewiring of transcriptional networks.
Predictors of warfarin use in atrial fibrillation in the United States: a systematic review and meta-analysis
Authors: Baczek Victoria L , Chen Wendy T , Kluger Jeffrey , Coleman Craig I
Published: February 2012

Background

Despite warfarin's marked efficacy, not all eligible patients receive it for stroke prevention in AF. The aim of this meta-analysis was to evaluate the association between prescriber and/or patient characteristics and subsequent prescription of warfarin for stroke prevention in patients with atrial fibrillation (AF).

Methods

Observational studies conducted in the US using multivariate analysis to determine the relationship between characteristics and the odds of receiving warfarin for stroke prevention were identified in MEDLINE, EMBASE and a manual review of references. Effect estimates of prescriber and/or patient characteristics from individual studies were pooled to calculate odds ratios (ORs) with 95% confidence intervals.

Results

Twenty-eight studies reporting results of 33 unique multivariate analyses were identified. Warfarin use across studies ranged from 9.1%-79.8% (median = 49.1%). There was a moderately-strong correlation between warfarin use and year of study (r = 0.60, p = 0.002). Upon meta-analysis, characteristics associated with a statistically significant increase in the odds of warfarin use included history of cerebrovascular accident (OR = 1.59), heart failure (OR = 1.36), and male gender (OR = 1.12). Those associated with a significant reduction in the odds of warfarin use included alcohol/drug abuse (OR = 0.62), perceived barriers to compliance (OR = 0.87), contraindication(s) to warfarin (OR = 0.81), dementia (OR = 0.32), falls (OR = 0.60), gastrointestinal hemorrhage (OR = 0.47), intracranial hemorrhage (OR = 0.39), hepatic (OR = 0.59), and renal impairment (OR = 0.69). While age per 10-year increase (OR = 0.78) and advancing age as a dichotomized variable (cut-off varied by study) (OR = 0.57) were associated with significant reductions in warfarin use; qualitative review of results of studies evaluating age as a categorical variable did not confirm this relationship.

Conclusions

Warfarin use has increased somewhat over time. The decision to prescribe warfarin for stroke prevention in atrial fibrillation is based upon multiple prescriber and patient characteristics. These findings can be used by family practice prescribers and other healthcare decision-makers to target interventions or methods to improve utilization of warfarin when it is indicated for stroke prevention.
Starmerella bombicola influences the metabolism of Saccharomyces cerevisiae at pyruvate decarboxylase and alcohol dehydrogenase level during mixed wine fermentation
Authors: Milanovic Vesna , Ciani Maurizio , Oro Lucia , Comitini Francesca
Published: February 2012

Background

The use of a multistarter fermentation process with Saccharomyces cerevisiae and non-Saccharomyces wine yeasts has been proposed to simulate natural must fermentation and to confer greater complexity and specificity to wine. In this context, the combined use of S. cerevisiae and immobilized Starmerella bombicola cells (formerly Candida stellata) was assayed to enhance glycerol concentration, reduce ethanol content and to improve the analytical composition of wine. In order to investigate yeast metabolic interaction during controlled mixed fermentation and to evaluate the influence of S. bombicola on S. cerevisiae, the gene expression and enzymatic activity of two key enzymes of the alcoholic fermentation pathway such as pyruvate decarboxylase (Pdc1) and alcohol dehydrogenase (Adh1) were studied.

Results

The presence of S. bombicola immobilized cells in a mixed fermentation trial confirmed an increase in fermentation rate, a combined consumption of glucose and fructose, an increase in glycerol and a reduction in the production of ethanol as well as a modification in the fermentation of by products. The alcoholic fermentation of S. cerevisiae was also influenced by S. bombicola immobilized cells. Indeed, Pdc1 activity in mixed fermentation was lower than that exhibited in pure culture while Adh1 activity showed an opposite behavior. The expression of both PDC1 and ADH1 genes was highly induced at the initial phase of fermentation. The expression level of PDC1 at the end of fermentation was much higher in pure culture while ADH1 level was similar in both pure and mixed fermentations.

Conclusion

In mixed fermentation, S. bombicola immobilized cells greatly affected the fermentation behavior of S. cerevisiae and the analytical composition of wine. The influence of S. bombicola on S. cerevisiae was not limited to a simple additive contribution. Indeed, its presence caused metabolic modifications during S. cerevisiae fermentation causing variation in the gene expression and enzymatic activity of alcohol deydrogenase and pyruvate decarboxilase.
Cytoadherence and virulence - the case of Plasmodium knowlesi malaria
Authors: Fatih Farrah A , Siner Angela , Ahmed Atique , Woon Lu Chan , Craig Alister G , Singh Balbir , Krishna Sanjeev , Cox-Singh Janet
Published: February 2012

Background

Cytoadherence of infected red blood cells to brain endothelium is causally implicated in malarial coma, one of the severe manifestations of falciparum malaria. Cytoadherence is mediated by specific binding of variant parasite antigens, expressed on the surface of infected erythrocytes, to endothelial receptors including, ICAM-1, VCAM and CD36. In fatal cases of severe falciparum malaria with coma, blood vessels in the brain are characteristically congested with infected erythrocytes. Brain sections from a fatal case of knowlesi malaria, but without coma, were similarly congested with infected erythrocytes. The objective of this study was to determine the binding phenotype ofPlasmodium knowlesiinfected human erythrocytes to recombinant human ICAM-1, VCAM and CD36.

Methods

Five patients with PCR-confirmedP. knowlesimalaria were recruited into the study with consent between April and August 2010. Pre-treatment venous blood was washed and culturedex vivoto increase the proportion of schizont-infected erythrocytes. Cultured blood was seeded into Petri dishes with triplicate areas coated with ICAM-1, VCAM and CD36. Following incubation at 37°C for one hour the dishes were washed and the number of infected erythrocytes bound/mm2to PBS control areas and to recombinant human ICAM-1 VCAM and CD36 coated areas were recorded. Each assay was performed in duplicate. Assay performance was monitored with thePlasmodium falciparumclone HB3.

Results

Blood samples were culturedex vivofor up to 14.5 h (mean 11.3 ± 1.9 h) to increase the relative proportion of mature trophozoite and schizont-infected red blood cells to at least 50% (mean 65.8 ± 17.51%). Three (60%) isolates bound significantly to ICAM-1 and VCAM, one (20%) isolate bound to VCAM and none of the five bound significantly to CD36.

Conclusions

Plasmodium knowlesiinfected erythrocytes from human subjects bind in a specific but variable manner to the inducible endothelial receptors ICAM-1 and VCAM. Binding to the constitutively-expressed endothelial receptor CD36 was not detected. Further work will be required to define the pathological consequences of these interactions.
Adaptation of Salmonella enterica Hadar under static magnetic field: effects on outer membrane protein pattern
Authors: Snoussi Sarra , May Alya El , Coquet Laurent , Chan Philippe , Jouenne Thierry , Landoulsi Ahmed , DÉ Emmanuelle
Published: February 2012

Background

Salmonella entericaserovar Hadar (S. Hadar) is a highly prevalent foodborne pathogen and therefore a major cause of human gastroenteritis worldwide. Outer membrane proteins whose production is often regulated by environmental conditions also play important roles in the adaptability of bacterial pathogens to various environments.

Results

The present study investigated the adaptation ofS. Hadar under the effect of acute static magnetic field exposure (200 mT, 9 h) and the impact on the outer membrane protein pattern.Viatwo-dimensional electrophoresis (2-DE) and LC-MS/MS spectrometry, we compared the proteome of enriched-outer membrane fraction before and after exposure to a magnetic field. A total of 11 proteins, displaying more than a two-fold change, were differentially expressed in exposed cells, among which 7 were up-regulated and 4 down-regulated. These proteins were involved in the integrity of cell envelope (TolB, Pal), in the response to oxidative stress (OmpW, dihydrolipoamide dehydrogenase, UspF), in the oxidative stress status (bacterioferritin), in virulence (OmpX, Yfgl) or in motility (FlgE and UspF). Complementary experiments associated the down-regulation of FlgE and UspF with an alteration of swarming, a flagella-driven motility, under SMF. Furthermore, the antibiotic disc diffusion method confirmed a decrease of gentamicin susceptibility in exposed cells. This decrease could be partly associated with the up-regulation of TolC, outer membrane component of an efflux pump. OmpA, a multifunctional protein, was up-regulated.

Conclusions

SMF (200 mT) seems to maintain the cell envelope integrity and to submit the exposed cells to an oxidative stress. Some alterations suggest an increase of the ability of exposed cells to form biofilms.
Reconfigurable parasitic antennas for compact mobile terminals in multiuser wireless systems
Authors: Barousis Vlasis I , Kanatas Athanasios G , Kalis Antonis , Perruisseau-Carrier Julien
Published: February 2012

This article considers the exploitation of parasitic antenna arrays in multi-user (MU) wireless communication systems by using their adaptive beamforming capabilities in order to improve the average system throughput. The use of parasitic arrays and especially the electrically steerable passive array radiator (ESPAR) antennas enables the design of terminals with a single RF front-end and reduced antenna dimensions, i.e., lightweight and compact mobile terminals. Although the beamforming capabilities of active element arrays at the receiver have been well investigated in the past, this article highlights the potentials of pattern reconfigurable parasitic arrays based on the beamspace representation of the ESPAR antenna. The advantages of using ESPAR at the receiving terminal are examined both in opportunistic beamforming and in MIMO broadcast channel MU systems, optimizing correspondingly the SNR or the SINR of the forward link.
Prehospital trauma care reduces mortality. Ten-year results from a time-cohort and trauma audit study in Iraq
Authors: Murad Mudhafar K , Larsen Stig , Husum Hans
Published: February 2012

Background

Blunt implementation of Western trauma system models is not feasible in low-resource communities with long prehospital transit times. The aims of the study were to evaluate to which extent a low-cost prehospital trauma system reduces trauma deaths where prehospital transit times are long, and to identify specific life support interventions that contributed to survival.

Methods

In the study period from 1997 to 2006, 2,788 patients injured by land mines, war, and traffic accidents were managed by a chain-of-survival trauma system where non-graduate paramedics were the key care providers. The study was conducted with a time-period cohort design.

Results

37% of the study patients had serious injuries with Injury Severity Score ≥ 9. The mean prehospital transport time was 2.5 hours (95% CI 1.9 - 3.2). During the ten-year study period trauma mortality was reduced from 17% (95% CI 15 -19) to 4% (95% CI 3.5 - 5), survival especially improving in major trauma victims. In most patients with airway problems, in chest injured, and in patients with external hemorrhage, simple life support measures were sufficient to improve physiological severity indicators.

Conclusion

In case of long prehospital transit times simple life support measures by paramedics and lay first responders reduce trauma mortality in major injuries. Delegating life-saving skills to paramedics and lay people is a key factor for efficient prehospital trauma systems in low-resource communities.
An in vivoinvestigation of the initiation and progression of subchondral cysts in a rodent model of secondary osteoarthritis
Authors: McErlain David D , Ulici Veronica , Darling Mark , Gati Joseph S , Pitelka Vasek , Beier Frank , Holdsworth David W
Published: February 2012

Introduction

Subchondral bone cysts (SBC) have been identified in patients with knee osteoarthritis (OA) as a cause of greater pain, loss of cartilage and increased chance of joint replacement surgery. Few studies monitor SBC longitudinally, and clinical research using three-dimensional imaging techniques, such as magnetic resonance imaging (MRI), is limited to retrospective analyses as SBC are identified within an OA patient cohort. The purpose of this study was to use dual-modality, preclinical imaging to monitor the initiation and progression of SBC occurring within an established rodent model of knee OA.

Methods

Eight rodents underwent anterior cruciate ligament transection and partial medial meniscectomy (ACLX) of the right knee. In vivo 9.4 T MRI and micro-computed tomography (micro-CT) scans were performed consecutively prior to ACLX and 4, 8, and 12 weeks post-ACLX. Resultant images were co-registered using anatomical landmarks, which allowed for precise tracking of SBC size and composition throughout the study. The diameter of the SBC was measured, and the volumetric bone mineral density (vBMD) was calculated within the bone adjacent to SBC. At 12 weeks, the ACLX and contralateral knees were processed for histological analysis, immunohistochemistry, and Osteoarthritis Research Society International (OARSI) pathological scoring.

Results

At 4 weeks post-ACLX, 75% of the rodent knees had at least 1 cyst that formed in the medial tibial plateau; by 12 weeks all ACLX knees contained SBC. Imaging data revealed that the SBC originate in the presence of a subchondral bone plate breach, with evolving composition over time. The diameter of the SBC increased significantly over time (P = 0.0033) and the vBMD significantly decreased at 8 weeks post-ACLX (P = 0.033). Histological analysis demonstrated positive staining for bone resorption and formation surrounding the SBC, which were consistently located beneath the joint surface with the greatest cartilage damage. Trabecular bone adjacent the SBC lacked viable osteocytes and, combined with bone marrow changes, indicated osteonecrosis.

Conclusions

This study provides insight into the mechanisms leading to SBC formation in knee OA. The expansion of these lesions is due to stress-induced bone resorption from the incurred mechanical instability. Therefore, we suggest these lesions can be more accurately described as a form of OA-induced osteonecrosis, rather than 'subchondral cysts'.
Mapping the magnetic susceptibility and electric conductivity of marine surficial sediments by benthic EM profiling
Authors: Müller Hendrik , von Dobeneck Tilo , Hilgenfeldt Christian , SanFilipo Bill , Rey Daniel , Rubio Belén
Published: February 2012

Distribution, accumulation, and diagenesis of surficial sediments in coastal and continental shelf systems follow complex chains of localized processes and form deposits of great spatial variability. Given the environmental and economic relevance of ocean margins, there is growing need for innovative geophysical exploration methods to characterize seafloor sediments by more than acoustic properties. A newly conceptualized benthic profiling and data processing approach based on controlled-source electromagnetic (CSEM) imaging permits to coevally quantify the magnetic susceptibility and the electric conductivity of shallow marine deposits. The two physical properties differ fundamentally insofar as magnetic susceptibility mostly assesses solid particle characteristics such as terrigenous or iron mineral content, redox state, and contamination level, while electric conductivity primarily relates to the fluid-filled pore space and detects salinity, porosity, and grain-size variations. We develop and validate a layered half-space inversion algorithm for submarine multifrequency CSEM with concentric sensor configuration. Guided by results of modeling, we modified a commercial land CSEM sensor for submarine application, which was mounted into a nonconductive and nonmagnetic bottom-towed sled. This benthic EM profiler Neridis II achieves 25 soundings/second at 3–4 knots over continuous profiles of up to a hundred kilometers. Magnetic susceptibility is determined from the 75 Hz in-phase response (90% signal originates from the top 50 cm), while electric conductivity is derived from the 5 kHz out-of-phase (quadrature) component (90% signal from the top 92 cm). Exemplary survey data from the north-west Iberian margin underline the excellent sensitivity, functionality, and robustness of the system in littoral (∼0–50 m) and neritic (∼50–300 m) environments. Susceptibility versus porosity crossplots successfully identify known lithofacies units and their transitions. All presently available data indicate an eminent potential of CSEM profiling for assessing the complex distribution of shallow marine surficial sediments and for revealing climatic, hydrodynamic, diagenetic, and anthropogenic factors governing their formation.
Accounting for soil surface roughness in the inversion of ultrawideband off-ground GPR signal for soil moisture retrieval
Authors: Jonard François , Weihermüller Lutz , Vereecken Harry , Lambot Sébastien
Published: February 2012

We combined a full-waveform ground-penetrating radar (GPR) model with a roughness model to retrieve surface soil moisture through signal inversion. The proposed approach was validated under laboratory conditions with measurements performed above a sand layer subjected to seven different water contents and four different surface roughness conditions. The radar measurements were performed in the frequency domain in the range of 1–3 GHz and the roughness amplitude standard deviation was varied from 0 to 1 cm. Two inversion strategies were investigated: (1) Full-waveform inversion using the correct model configuration, and (2) inversion focused on the surface reflection only. The roughness model provided a good description of the frequency-dependent roughness effect. For the full-waveform analysis, accounting for roughness permitted us to simultaneously retrieve water content and roughness amplitude. However, in this approach, information on soil layering was assumed to be known. For the surface reflection analysis, which is applicable under field conditions, accounting for roughness only enabled water content to be reconstructed, but with a root mean square error (RMS) in terms of water content of [Formula: see text] compared to an RMS of [Formula: see text] for an analysis where roughness is neglected. However, this inversion strategy required a priori information on soil surface roughness, estimated, e.g., from laser profiler measurements.