bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of polypeptides in bee venom on growth inhibition and apoptosis induction of the human hepatoma cell line SMMC-7721 in-vitro and Balb/c nude mice in-vivo
Authors: Hu H , Chen D , Li Y , Zhang X
Published: December 2005

Polypeptides in bee venom (PBV) produced a significant growth inhibition against SMMC-7721 human hepatoma cell line. Analysis of the mechanisms of cell death indicated that PBV induced an apoptotic cell death. SMMC-7721 cells exposed to PBV (10.0 μg mL−1) produced an insignificant morphological change. Analysis of the cytotoxicity with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium) assay confirmed that the cytotoxic effects of PBV were dose- and time-dependent. The result of Ki67 immunohistochemistry demonstrated that the proliferation of SMMC-7721 cells treated with PBV (10.0 μg mL−1) was inhibited. The apoptotic cell death was then confirmed by annexin V, propidium iodide staining and DNA fragmentation analysis. In in-vivo experiments, treatment with PBV (1.5 or 3 mg kg−1) resulted in a significant retardation of SMMC-7721 cell growth in Balb/c nude mice. These findings suggested that PBV could be used as a chemotherapeutic agent against tumours.
Effect of tranilast on matrix metalloproteinase production from neutrophils in-vitro
Authors: Shimizu Toshiyuki , Kanai Ken-Ichi , Kyo Yoshiyuki , Suzaki Harumi , Asano Kazuhito , Hisamitsu Tadashi
Published: December 2005

Tranilast is an anti-allergic agent that blocks the release of chemical mediators, such as histamine and leukotrienes from mast cells, and has been reported to suppress keloid and hypertrophic scar formation. Since matrix metalloproteinases (MMPs) play an essential role in tissue remodelling, this study was undertaken to determine whether tranilast suppresses MMP production from neutrophils after lipopolysaccharide (LPS) stimulation in-vitro. Neutrophils from five healthy donors (1times105 cells/mL) were stimulated with 1.0 μg mL−1 LPS in the presence or absence of various concentrations of tranilast for 24 h. MMP-7, MMP-8, MMP-9 and tissue inhibitor of metalloproteinase (TIMP)-1 levels in the culture supernatants were assayed by ELISA. In addition, the influence of tranilast on MMP mRNA expression and transcriptional factor activation in cells cultured for 12 h and 4 h was also evaluated by reverse transcriptase—polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Tranilast inhibited MMP and TIMP-1 production from neutrophils when cells were treated with the agent at more than 5.0times10−5  m. It also suppressed MMP mRNA expression and transcriptional factor activation induced in neutrophils by LPS stimulation. The results suggest that tranilast inhibits the formation of keloid scarring through the suppression of factors such as MMPs and TIMP, which are essential for tissue remodelling, from inflammatory cells.
Effects of some natural 5-hydroxy-isoflavones on cultured human endothelial cells in presence and absence of hydrogen peroxide
Authors: Paulo Alexandra , Mota-Filipe Helder
Published: December 2005

Due to their biological activity, it has been suggested that consumption of isoflavone-rich diets may prevent prostate and breast cancers, osteoporosis and cardiovascular diseases. Preventive effects of isoflavones on cancer and cardiovascular diseases have been associated with their oestrogenic and antioxidant properties. However, concerns still exist about the potential dangers of consuming high levels of these compounds, since it is known that some of them have cytostatic or cytotoxic properties, depending on the concentration. To evaluate the potential cytotoxic risk and antioxidant benefit of natural 5-hydroxy-isoflavones (5-OH-isoflavones) for human vascular endothelium, the effect of some natural 5-OH-isoflavones was evaluated on cultured human endothelial cells, in the presence and absence of H2O2 (3 mm for 4 h). None of the isoflavones tested were able to prevent oxidative damage to endothelial cells at maximal extracellular concentrations of 1 mm. The low antioxidant capacity of these compounds was also shown by the DPPH (1,1-diphenyl-2-picrylhydrazyl radical) method. On the other hand, genistein and biochanin A, having a free 7-OH group, were toxic to the human endothelial cells in a dose-dependent manner, at concentrations ≥300 μm and ≥ 100 μm, respectively. These results indicate that the non-specific cytotoxic effect of 5-OH-isoflavones is associated with the free 7-OH group. In conclusion, we were not able to show that 5-OH-isoflavones are beneficial to human endothelial cells when the cells were exposed to oxidative stress caused by 3 mm of H2O2, but it can be concluded that consumption of 5-OH-isoflavones is of no direct cytotoxic risk to the human vascular endothelium since toxic concentrations are believed to be unreachable in-vivo.
Antinociceptive properties of coumarins, steroid and dihydrostyryl-2-pyrones from Polygala sabulosa (Polygalaceae) in mice
Authors: Ardenghi Juliana V , Pretto Juliana B , Souza Márcia M , Junior Anildo Cunha , Soldi Cristian , Pizzolatti Moacir Geraldo , Meotti Flavia Carla , Moura Janaína d‘Ávila , Santos Adair R S
Published: December 2005

We have investigated the possible antinociceptive action of the extract, fractions and pure compounds obtained from the whole plant Polygala sabulosa A. W. Bennett (Polygalaceae) in acetic acid-induced visceral pain in mice. Intraperitoneal injection of animals with the hydroalcoholic extract and fractions (CH2Cl2, EtOAc, n-BuOH, aqueous fraction) (1–100 mg kg−1) caused a dose-related and significant inhibition of the acetic acid-induced visceral nociceptive response. The CH2Cl2, EtOAc and n-BuOH fractions were more potent than the hydroalcoholic extract and aqueous fraction. The isolated compounds dihydrostyryl-2-pyrones (1, 2, 3), styryl-2-pyrone (7), α-spinasterol (9), scopoletin (10) and two esters of the coumarin (scopoletin) obtained semisynthetically, acetylscopoletin (10a) and benzoylscopoletin (10b) (0.001–10 mg kg−1), exhibited significant and dose-related antinociceptive effects against acetic acid-induced visceral pain. The results distinguished, for the first time, the extract, fractions and pure compounds obtained from P. sabulosa that produced marked antinociception against the acetic acid-induced visceral nociceptive response, supporting the ethnomedical use of P. sabulosa.
Radical scavenging and anti-inflammatory activity of extracts from Opuntia humifusa Raf.
Authors: Cho J Y , Park S-C , Kim T-W , Kim K-S , Song J-C , Lee H-M , Sung H-J , Rhee M-H , Kim S-K , Park H-J , Song Y-B , Yoo E-S , Lee C-H
Published: December 2005

Opuntia humifusa Raf. (O. humifusa Raf.) is a member of the Cactaceae family. To determine the antioxidative and anti-inflammatory effects of this herb, various solvent fractions (methanol, hexane, chloroform, ethyl acetate, butanol, and water) prepared from the leaves of cacti were tested using DPPH (2,2-diphenyl-l-picrylhydrazyl radical) and xanthine oxidase assays, and nitric oxide (NO)-producing macrophage cells. We found that O. humifusa Raf. displayed potent antioxidative and anti-inflammatory activity. Thus, all solvent fractions, except for the water layer, showed potent scavenging effects. The scavenging effect of the ethyl acetate fraction was higher than that of the other fractions, with IC50 values of 3.6 and 48.2 μg mL−1. According to activity-guided fractionation, one of the active radical scavenging principles in the ethyl acetate fraction was found to be quercetin. In contrast, only two fractions (chloroform and ethyl acetate) significantly suppressed nitric oxide production from the lipopolysaccharide (LPS)-activated RAW264.7 cells. In addition, chloroform and ethyl acetate fractions significantly blocked the expression of inducible nitric oxide synthetase (iNOS) and interleukin-6 (IL-6) from the RAW264.7 cells stimulated by LPS. Moreover, ethyl acetate fractions significantly blocked the expression of IL-1β from the RAW264.7 cells stimulated by LPS. Therefore, the results suggested that O. humifusa Raf. may modulate radical-induced toxicity via both direct scavenging activity and the inhibition of reactive species generation, and the modulation of the expression of inflammatory cytokines. Finally, O. humifusa Raf. may be useful as a functional food or drug against reactive species-mediated disease.
Hypoglycaemic and hypolipidaemic effects of fractions from kolaviron, a biflavonoid complex from Garcinia Kola in streptozotocin-induced diabetes mellitus rats
Authors: Adaramoye O A , Adeyemi E O
Published: December 2005

In the search for natural hypoglycaemic agents as alternatives to synthetic ones that are expensive and not easily accessible, and to justify the use of Garcinia kola seeds in traditional African medicine to treat diabetes, the hypoglycaemic and hypolipidaemic effects of fractions from kolaviron (KV) (a Garcinia kola seed extract) were investigated in normal and streptozotocin (STZ)-diabetic rats. KV, a biflavonoid complex from Garcinia kola seed, was separated by thin-layer chromatography into three fractions; Fraction I (FI), Fraction II (FII) and Fraction III (FIII) with RF values of 0.48, 0.71 and 0.76, respectively. In normoglycaemic rats, KV, FI and FII administered at a dose of 100 mg kg−1 body weight elicited significant (P < 0.05) hypoglycaemic activity within 4 h of oral administration. Precisely, KV, FI and FII decreased blood glucose levels of normoglycaemic rats by 66%, 50% and 61%, respectively, when compared with controls 30 min after oral administration of the extracts. In hyperglycaemic rats, KV, FI and FII significantly (P < 0.05) reduced blood sugar levels in STZ-diabetic rats within 4 h of oral administration. Furthermore, KV alone produced a significant (P < 0.05) anti-diabetic effect from day 3 to day 7 of oral intubation of STZ-diabetic rats. In addition, the extracts showed favourable effect on the plasma lipid profile of STZ-diabetic rats, and also decreased significantly (P < 0.05) the STZ-induced increase in the activity of microsomal glucose-6-phosphatase and lipid peroxidation (LPO) products. This study confirms the anti-diabetic and hypo-lipidaemic effects of KV in STZ-diabetic rats. These observed effects of KV are attributed to two of its fractions, FI and FII, with RF values of 0.48 and 0.71, respectively.
The inhibitory effect of phenylpropanoid glycosides and iridoid glucosides on free radical production and β2 integrin expression in human leucocytes
Authors: Lin Lie-Chwen , Shen Yuh-Chiang , Wang Yea-Hwey , Liou Kuo-Tong , Hou Yu-Chang , Chang Shiou , Wang Wen-Yen , Chou Yueh-Ching
Published: December 2005

Rapid production of reactive oxygen species (ROS) and upregulation of β2 integrin by leucocytes are two important inflammatory responses in human leucocytes. To evaluate whether three phenylpropanoid glycosides (acteoside, crenatoside, and rossicaside B) and two iridoid glucosides (boschnaloside and 8-epideoxyloganic acid) identified from two medicinal plants with similar indications (Orobanche caerulescens and Boschniakia rossica) exhibited anti-inflammatory activity, their effects on N-formyl-methionyl-leucyl-phenylalanine (fMLP) and phorbol-12-myristate-13-acetate (PMA)-activated peripheral human neutrophils (PMNs) and mononuclear cells were examined. Pretreatment with 1–50 μm phenylpropanoid glycoside concentration-dependently diminished PMA- and fMLP-induced ROS production with IC50 values of approximately 6.8–23.9 and 3.0–8.8 μm, respectively. Iridoid glucoside was less effective than phenylpropanoid glycoside with an IC50 value of approximately 8.9–28.4 μm in PMA-activated PMNs and 19.1–21.1 μm in fMLP-activated mononuclear cells. Phenylpropanoid glycosides also effectively inhibited NADPH oxidase (NOX) and displayed potent free radical-scavenging activity, but did not interfere with pan-protein kinase C (PKC) activity. Furthermore, all compounds, except rossicaside B, significantly inhibited PMA- and fMLP-induced Mac-1 (a β2 integrin) upregulation at 50 μm but not that of fMLP-induced intracellular calcium mobilization. These drugs had no significant cytotoxicity as compared with the vehicle control. Our data suggested that inhibition of ROS production, possibly through modulation of NOX activity and/or the radical scavenging effect, and β2 integrin expression in leucocytes indicated that these compounds had the potential to serve as anti-inflammatory agents during oxidative stress.
Protective effect of crude extract from Wedelia paludosa (Asteraceae) on the hepatotoxicity induced by paracetamol in mice
Authors: Rosa Juliana Martins , Brocardo Patrícia S , Balz Daniela , Rodrigues Ana Lúcia S , Waltrick Ana Paula , Bagio Angelize , Goulart Eduardo Comeli , Meotti Flavia Carla , Dafre Alcir Luiz , Santos Adair R S
Published: December 2005

Several in-vitro and in-vivo ethnopharmacological studies carried out with plants of the genus Wedelia have already demonstrated hepatoprotective effects in chemically-induced liver injury, including those induced by paracetamol. Here, the effects of the crude extract from Wedelia paludosa on paracetamol-induced hepatotoxicity in mice was investigated. Intraperitoneal injection of paracetamol (1000 mg kg−1) caused 80% death after 24 h in mice, which was significantly reduced by oral pretreatment with W. paludosa (500 mg kg−1). Hepatotoxicity was observed 24 h after an intraperitoneal injection of paracetamol (600 mg kg−1), as evidenced by an increase in plasma activity of aspartate and alanine aminotransferases. That hepatotoxicity was significantly attenuated by W. paludosa pretreatment (100–500 mg kg−1) in a dose-response manner. Paracetamol (1000 mg kg−1) drastically depleted total glutathione levels and decreased glutathione peroxidase and δ-aminolevulinate dehydratase activity in the liver, such effects not being prevented by pretreatment with W. paludosa. Neither paracetamol treatment alone nor pretreatment with W. paludosa altered glutathione reductase and glutathione S-transferase activity or the levels of end-products of lipid peroxidation. In conclusion, we found that W. paludosa protected against paracetamol-induced hepatotoxicity, an effect not observed over oxidative stress-related parameters. Hepatoprotection is likely mediated by some terpenes present in W. paludosa extract. However, further studies will be required to explain the mechanisms involved in the hepatoprotection afforded by W. paludosa.
Protective effect of glycoprotein isolated from Ulmus davidiana Nakai on carbon tetrachloride-induced mouse liver injury
Authors: Lee Sei-Jung , Oh Phil-Sun , Ko Jeong-Hyeon , Lim Kye-Taek , Lim Kwang
Published: December 2005

This study was carried out to evaluate the hepatoprotective activity of glycoprotein isolated from the stems of Ulmus davidiana Nakai (UDN), which has been used as an anti-inflammatory agent in folk medicine. We evaluated lipid peroxidation in glucose/glucose oxidase (G/GO)-induced BNL CL.2 cells and measured thiobarbituric acid reactive substances (TBARS), lactate dehydrogenase (LDH), nitric oxide (NO), antioxidant enzyme (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)), activity of cytotoxic-related signals (hepatic cytochrome c, nuclear factor-kappa B (NF-κB) and activator protein-1 (AP-1)) and levels of plasma lipids (triglyceride (TG) and total cholesterol (TC)) in carbon tetrachloride (CCl4, 1.0 mL kg−1)-induced A/J mouse. The results in G/GO-induced BNL CL.2 cells showed that UDN glycoprotein had a dose-dependent inhibitory effect on lipid peroxidation. The results in carbon tetrachloride (CCl4, 1.0 mL kg−1)-induced A/J mouse indicated that treatment with UDN glycoprotein (40 mg kg−1) lowered LDH activity and TBARS formation, and increased NO production and antioxidant enzymes activity, compared with control. Also, our finding from CCl4-treated mice after pretreatment with UDN glycoprotein demonstrated that the activity of cytotoxic-related signals decreased but the levels of plasma lipids increased, compared with CCl4 treatment alone. Here, we speculate that UDN glycoprotein has a protective character to CCl4-induced mouse liver injury.
Comparing ultrasound measurements of neonatal hips using the methods of Graf and Terjesen
Authors: Falliner A. , Schwinzer D. , Hahne H.-J. , Hedderich J. , Hassenpflug J.
Published: December 2005

In a prospective study, 232 neonates were examined sonographically using the methods of Graf and Terjesen. In order to determine the reproducibility of the methods, 50 hips were evaluated by two skilled examiners. In an inter-observer study, five physicians and five medical students evaluated 24 images, which were evaluated on ten occasions at two-weekly intervals by one of the authors. Statistical evaluation used the Bland-Altman approach.

The neonates (110 females, 122 males) were less than four days old. The mean α angle was 62.4° and mean femoral head cover was 55.4%. According to Graf’s method, 1.3% of hips were pathological, compared with 4.1% according to Terjesen. Spearman’s correlation coefficient between femoral head cover and α angles was 0.552. The Bland-Altman approach shows greater variation for femoral head cover than for α, if measured by experienced examiners. The Bland-Altman approach shows almost equal reproducibility for α and femoral head cover in the inter-observer test, but better repeatability for α in the intra-observer test.

The Graf results relate better than Terjesen’s to the well-known frequency of 1% to 2% hip dysplasia in the European population. Kappa statistics indicate a fair agreement between the two methods. Inter-observer evaluation shows an equal reproducibility of both methods, whereas intra-observer tests reveal better repeatability with Graf’s method.
Revision of failed total elbow arthroplasty with use of a linked implant
Authors: Sneftrup S. B. , Jensen S. L. , Johannsen H. V. , Søjbjerg J. O.
Published: December 2005

We studied retrospectively the results of revision arthroplasty of the elbow using a linked Coonrad-Morrey implant in 23 patients (24 elbows) after a mean follow-up period of 55 months. According to the Mayo Elbow Performance Score, 19 elbows were satisfactory, nine were excellent and ten good. The median total score had improved from 35 points (20 to 75) before the primary arthroplasty to 85 points (40 to 100) at the latest follow-up. There was a marked relief of pain, but the range of movement showed no overall improvement. Two patients had a second revision because of infection and two for aseptic loosening. The estimated five-year survival rate of the prosthesis was 83.1% (95% confidence interval 61.1 to 93.3). Revision elbow arthroplasty using the Coonrad-Morrey implant provided satisfactory results but with complications occurring in 13 cases.
A comparison of the shrinkage of commercial bone cements when mixed under vacuum
Authors: Kwong F. N. K. , Power R. A.
Published: December 2005

The outcome of a cemented hip arthroplasty is partly dependent on the type of cement which is used. The production of an interface gap between the stem and the cement mantle as a result of shrinkage of the cement, may be a factor involved. Palacos R, Palacos LV (both with gentamicin), CMW 1, CMW 2, CMW Endurance (CMWE) and Simplex were prepared under vacuum and allowed to cure overnight in similar cylinders. The next day this volume was determined by the displacement of water. Shrinkage varied between 3.82% and 7.08% with CMWE having the lowest and Palacos LV the highest. This could be a factor to consider when choosing a cement for a shape-closed stem.
A prospective comparison of clamping the drain or post-operative salvage of blood in reducing blood loss after total knee arthroplasty
Authors: Tsumara N. , Yoshiya S. , Chin T. , Shiba R. , Kohso K. , Doita M.
Published: December 2005

We undertook a prospective, randomised study in order to evaluate the efficacy of clamping the drains after intra-articular injection of saline with 1:500 000 adrenaline compared with post-operative blood salvage in reducing blood loss in 212 total knee arthroplasties. The mean post-operative drained blood volume after drain clamping was 352.1 ml compared to 662.3 ml after blood salvage (p < 0.0001). Allogenic blood transfusion was needed in one patient in the drain group and for three in the blood salvage group. Drain clamping with intra-articular injection of saline with adrenaline is more effective than post-operative autologous blood transfusion in reducing blood loss during total knee arthroplasty.
The posterior Monteggia lesion with associated ulnohumeral instability
Authors: Strauss E. J. , Tejwani N. C. , Preston C. F. , Egol K. A.
Published: December 2005

The type II Monteggia (posterior) lesion is a rare injury which is sometimes associated with ulnohumeral instability. We have reviewed 23 of 28 patients with this injury. A clinical and radiographic assessment was undertaken at follow-up. Functional outcome scores, including the Broberg and Morrey Index and the Disabilities of the Arm, Shoulder or Hand (DASH), were used. The results from the six patients with associated posterior ulnohumeral dislocation were compared with 17 without ulnohumeral injury. Those with dislocation had reduced movement of the elbow and had outcome scores indicative of greater disability compared to those without associated dislocation.
Removal of acetabular bone in resurfacing arthroplasty of the hip
Authors: Loughead J. M. , Starks I. , Chesney D. , Matthews J. N. S. , McCaskie A. W. , Holland J. P.
Published: December 2005

Resurfacing arthroplasty of the hip is being performed more frequently in the United Kingdom. The majority of these patients are younger than 55 years of age, and in this group the key benefits include conservation of femoral bone stock and the potential reduction in the rate of dislocation afforded by the larger resurfacing head. Early aseptic loosening is well recognised in patients younger than 55 years of age, and proponents of resurfacing believe that the improved wear characteristics of the metal-on-metal bearing may improve the long-term survival of this implant. There has been some concern, however, that resurfacing may not be conservative of acetabular bone.

We compared a series of 33 consecutive patients who had a hybrid total hip arthroplasty with an uncemented acetabular component and a cemented femoral implant, with 35 patients undergoing a Birmingham hip resurfacing arthroplasty. We compared the diameter of the implanted acetabulum in both groups and, because they were not directly comparable, we corrected for patient size by measuring the diameter of the contralateral femoral head. The data were analysed using unpaired t-tests and analysis of covariance.

There was a significantly larger acetabulum in the Birmingham arthroplasty group (mean diameter 56.6 mm vs 52.0 mm; p < 0.001). However, this group had a significantly larger femoral head diameter on the contralateral side (p = 0.03). Analysis of covariance revealed a significant difference between the mean size of the acetabular component implanted in the two operations. The greatest difference in the size of acetabulum was in those patients with a larger diameter of the femoral head. This study shows that more bone is removed from the acetabulum in hip resurfacing than during hybrid total hip arthroplasty, a difference which is most marked in larger patients.
Clinical outcome of nerve injuries associated with supracondylar fractures of the humerus in children
Authors: Ramachandran M. , Birch R. , Eastwood D. M.
Published: December 2005

Between 1998 and 2002, 37 neuropathies in 32 patients with a displaced supracondylar fracture of the humerus who were referred to a nerve injury unit were identified. There were 19 boys and 13 girls with a mean age of 7.9 years (3.6 to 11.3). A retrospective review of these injuries was performed. The ulnar nerve was injured in 19, the median nerve in ten and the radial nerve in eight cases. Fourteen neuropathies were noted at the initial presentation and 23 were diagnosed after treatment of the fracture. After referral, exploration of the nerve was planned for 13 patients. Surgery was later cancelled in three because of clinical recovery. Six patients underwent neurolysis alone. Excision of neuroma and nerve grafting were performed in four. At follow-up, 26 patients had an excellent, five a good and one a fair outcome.
Obstructive sleep apnoea syndrome in morbidly obese children with tibia vara
Authors: Gordon J. E. , Hughes M. S. , Shepherd K. , Szymanski D. A. , Schoenecker P. L. , Parker L. , Uong E. C.
Published: December 2005

Morbid obesity and its association with obstructive sleep apnoea syndrome have been increasingly recognised in children. Orthopaedic surgeons are often the primary medical contact for older children with tibia vara, which has long been associated with obesity, but are unfamiliar with the evaluation and treatment of sleep apnoea in children.

We reviewed all children with tibia vara treated surgically at one of our institutions over a period of five years. Thirty-seven patients were identified; 18 were nine years of age or older and 13 of these (72%) had morbid obesity and a history of snoring.

Eleven children were diagnosed as having sleep apnoea on polysomnography. The incidence of this syndrome in the 18 children aged nine years or older with tibia vara, was 61%. All these patients required pre-operative non-invasive positive-pressure ventilation; tonsillectomy and adenoidectomy were necessary in five (45%). No peri-operative complications related to the airway occurred.

There is a high incidence of sleep apnoea in morbidly obese patients with tibia vara. These patients should be screened for snoring and, if present, should be further evaluated for sleep apnoea before corrective surgery is undertaken.
Assessment of a self-administration protocol for extended subcutaneous thromboprophylaxis in lower limb arthroplasty
Authors: Watts A. C. , Howie C. R. , Simpson A. H. R. W.
Published: December 2005

The risk of venous thromboembolism in patients following arthroplasty may be reduced by continuing chemical thromboprophylaxis for up to 35 days post-operatively. This prospective cohort study investigated the compliance of 40 consecutive consenting patients undergoing lower limb arthroplasty with self-administration of a recommended subcutaneous chemotherapeutic agent for six weeks after surgery. Compliance was assessed by examination of the patient for signs of injection, number of syringes used, and a self-report diary at the end of the six-week period. A total of 40 patients, 15 men and 25 women, were recruited. One woman was excluded because immediate post-operative complications prevented her participation. Self-administration was considered feasible in 87% of patients (95% confidence interval (CI) 76 to 98) at the time of discharge. Among this group of 34 patients, 29 (85%) were compliant (95% CI 73 to 97). Patients can learn to self-administer subcutaneous injections of thromboprophylaxis, and compliance with extended prophylaxis to six weeks is good.
Risk factors for failure after treatment of a periprosthetic fracture of the femur
Authors: Lindahl H. , Malchau H. , Odén A. , Garellick G.
Published: December 2005

Periprosthetic fracture of the femur is an uncommon complication after total hip replacement, but appears to be increasing. We undertook a nationwide observational study to determine the risk factors for failure after treatment of these fractures, examining patient- and implant-related factors, the classification of the fractures and the outcome.

Between 1979 and 2000, 1049 periprosthetic fractures of the femur were reported to the Swedish National Hip Arthroplasty Register. Of these, 245 had a further operation after failure of their initial management. Data were collected from the Register and hospital records. The material was analysed by the use of Poisson regression models.

It was found that the risk of failure of treatment was reduced for Vancouver type B2 injuries (p = 0.0053) if revision of the implant was undertaken (p = 0.0033) or revision and open reduction and internal fixation (p = 0.0039) were performed. Fractures classified as Vancouver type B1 had a significantly higher risk of failure (p = 0.0001). The strongest negative factor was the use of a single plate for fixation (p = 0.001). The most common reasons for failure in this group were loosening of the femoral prosthesis, nonunion and re-fracture.

It is probable that many fractures classified as Vancouver type B1 (n = 304), were in reality type B2 fractures with a loose stem which were not recognised. Plate fixation was inadequate in these cases. The difficulty in separating type B1 from type B2 fractures suggests that the prosthesis should be considered as loose until proven otherwise.
The Oxford medial unicompartmental knee replacement using a minimally-invasive approach
Authors: Pandit H. , Jenkins C. , Barker K. , Dodd C. A. F. , Murray D. W.
Published: December 2005

This prospective study describes the complications and survival of the first 688 Phase 3 Oxford medial unicompartmental knee replacements implanted using a minimally-invasive technique by two surgeons and followed up independently. None was lost to follow-up. We had carried out 132 of the procedures more than five years ago. The clinical assessment of 101 of these which were available for review at five years is also presented.

Nine of the 688 knees were revised: four for infection, three for dislocation of the bearing and two for unexplained pain. A further seven knees (1%) required other procedures: four had a manipulation under anaesthesia, two an arthroscopy and one a debridement for superficial infection. The survival rate at seven years was 97.3% (95% confidence interval 5.3). At five years, 96% of the patients had a good or excellent American Knee Society score, the mean Oxford knee score was 39 and the mean flexion was 133°. This study demonstrates that the minimally-invasive Oxford unicompartmental knee replacement is a reliable and effective procedure.
Maintaining standards in surgical training
Authors: Jain N. , Willett K. M.
Published: December 2005

In order to assess the efficacy of inspection and accreditation by the Specialist Advisory Committee for higher surgical training in orthopaedic surgery and trauma, seven training regions with 109 hospitals and 433 Specialist Registrars were studied over a period of two years.

There were initial deficiencies in a mean of 14.8% of required standards (10.3% to 19.2%). This improved following completion of the inspection, with a mean residual deficiency in 8.9% (6.5% to 12.7%.) Overall, 84% of standards were checked, 68% of the units improved and training was withdrawn in 4%.

Most units (97%) were deficient on initial assessment. Moderately good rectification was achieved but the process of follow-up and collection of data require improvement. There is an imbalance between the setting of standards and their implementation. Any major revision of the process of accreditation by the new Post-graduate Medical Education and Training Board should recognise the importance of assessment of training by direct inspection on site, of the relationship between service and training, and the advantage of defining mandatory and developmental standards.
Synthetic methodology using tertiary phosphines as nucleophilic catalysts
Authors: Lu Xiyan , Du Yishu , Lu Cheng
Published: December 2005

Allenoates or 2-alkynoates are known to react with tertiary phosphines to form the phosphine-substituted 1,3-dipoles, which can react with various substrates with the simultaneous elimination of the tertiary phosphine. The reaction is catalytic to the tertiary phosphine used. The investigation of the appropriate dipolarophiles and the further extension of this reaction are discussed.
Recent developments in the chemistry of low-coordinated organophosphorus compounds
Authors: Yoshifuji Masaaki
Published: December 2005

Sterically protected organophosphorus compounds are described, involving diphosphenes, phosphaethenes, diphosphinidenecyclobutenes (DPCBs), phosphaalkynes, phosphaquinones, diphosphathienoquinones, and so on of coordination number 2 or 1. Application of the DPCBs as well as phosphinophosphaethenes as a ligand of transition-metal catalysts for several organic coupling reactions has been investigated.
Transition-metal-catalyzed reactions of carbon-heteroatom bond formation by substitution and addition processes
Authors: Beletskaya Irina P.
Published: December 2005

Two types of transition-metal-catalyzed cross-coupling reactions, which both lead to the formation of carbon-heteroatom bonds, are considered: RX + E-H and E-X + RM. The potential of addition reactions of E-H or E-E to double or triple bond in C-E bond formation is also demonstrated.
Thiocarbonyl-based 1,3-dipolarophiles for the synthesis of C(2)-unsubstituted penems
Authors: Gallagher Timothy , Sanchez Sylvie , Bateson John H. , O'Hanlon Peter J.
Published: December 2005

The azomethine ylid strategy for β-lactam synthesis, which involves the fragmentation of a β-lactam-based oxazolidinone to give a carboxylated azomethine ylid, has been extended to the C(2)-unsubstituted penem scaffold associated with C(6)-exoalkylidene penems. This involved the use of S-methyl dithioformate as a reactive 1,3-dipolarophile, which was most effectively employed by initial trapping with cyclopentadiene and subsequent regeneration of S-methyl dithioformate via a retro Diels-Alder reaction in the presence of the requisite oxazolidinone. This provided access to the penam scaffold, and this overall sequence was very effectively accelerated by microwave irradiation. The synthesis of C(2)-unsubstituted penems was then accomplished by application of an oxidative elimination sequence.
Discovery of fused azetidines as novel selective α4β2 neuronal nicotinic receptor (NNR) agonists
Authors: Ji Jianguo , Bunnelle William H. , Li Tao , Pace Jennifer M. , Schrimpf Michael R. , Sippy Kevin B. , Anderson David J. , Meyer Michael D.
Published: December 2005

An efficient synthesis of (1R,5S)-6-(5-cyano-3-pyridinyl)-3,6-diaza-bicyclo[3.2.0]heptane A-366833, a novel potent selective neuronal nicotinic receptor (NNR) agonist, is described. The enantiomerically pure pharmacophore benzyl (1S,5S)-3,6-diaza-bicyclo[3.2.0]heptane-3-carbamate was successfully constructed from benzyl N-allyl-N-(2-hydroxyimino-ethyl)-carbamate through a convenient approach including an intramolecular [1,3]-dipolar cycloaddition, reductive ring-opening reaction, chiral resolution, and intramolecular cyclization. Subsequent N-arylation of the pharmacophore with 3-bromo-5-cyanopyridine and N-Cbz deprotection with trifluoroacetic acid furnished A-366833.
Recent progress in Lewis acid-Lewis base bifunctional asymmetric catalysis
Authors: Kanai Motomu , Kato Nobuki , Ichikawa Eiko , Shibasaki Masakatsu
Published: December 2005

Two enantioselective cyanation reactions, the Strecker reaction of ketoimines and the Reissert reaction of pyridine derivatives, promoted by Lewis acid-Lewis base bifunctional asymmetric catalysts are described.
Dual enantioselective control by heterocycles of (S)-indoline derivatives
Authors: Kim Yong Hae , Jung Doo Young , Youn So Won , Kim Sam Min , Park Doo Han
Published: December 2005

Diastereo-and enantioselective pinacol coupling reactions of chiral α-ketoamides mediated by samarium diiodide afforded extremely high diastereoselectivities. Enantiopure (S,S)- or (R,R)-2,3-dialkyltartaric acid and derivatives can be synthesized. Diels-Alder cycloadditions of S-indoline chiral acrylamides with cyclopentadiene in the presence of Lewis acids proceed with high diastereofacial selectivity, giving either endo-R or endo-S products depending on Lewis acid and the structures of chiral dienophiles.
New reactions of functionalized β-lactams
Authors: Van Brabandt Willem , Dejaegher Yves , De Kimpe Norbert
Published: December 2005

An easy synthesis of 4-(1- and 2-haloalkyl)azetidin-2-ones by a [2+2] cycloadditon protocol is described. Starting from these functionalized β-lactams, new pathways toward bicyclic β-lactams were developed via intramolecular nucleophilic substitutions and radical cyclizations. 4-(1- and 2-Haloalkyl)azetidin-2-ones also proved to be very useful starting products in the synthesis of various highly substituted aziridines, azetidines, and alkenoates.
From vinylsilanes to organic/inorganic hybrid materials
Authors: Luh Tien-Yau
Published: December 2005

Alternating regioregular silylene-spaced divinylarene copolymers [(donor-SiMe2-)
n

=1-3-(acceptor-SiMe2)] are synthesized by rhodium-catalyzed hydrosilylation of bis-alkynes with bisvinylic silyl hydrides. Efficient intrachain energy transfer has been observed in these copolymers. The intensities of emission profiles depend on the relative ratio of donor-to-acceptor chromophores. The corresponding inorganic/organic hybrid materials are synthesized, and their photophysical properties are described.
Asymmetric cyclopropanation of chiral (1-phosphoryl)vinyl sulfoxides: A new approach to constrained analogs of biologically active compounds
Authors: Mikołajczyk Marian
Published: December 2005

This account outlines the results obtained in the author's laboratory on the asymmetric cyclopropanation of enantiopure 1-phosphorylvinyl p-tolyl sulfoxides with sulfur ylides and diazoalkanes. Based on experimental results and theoretical calculations, the transition-state model for asymmetric cyclopropanation is proposed. A great synthetic value of the reaction investigated is exemplified by the total synthesis of constrained analogs of bioactive compounds, namely, enantiopure cyclic analog of phaclofen and cyclopropylphosphonate analogs of nucleotides.
π-Conjugated systems: Can phosphole offer more than pyrrole?
Authors: Hissler Muriel , Lescop Christophe , Réau Régis
Published: December 2005

An overview of the synthesis, physical properties, and use as material for organic light-emitting diodes (OLEDs) of linear organic π-conjugated systems incorporating phosphole and thiophene rings is presented.
Chiral phosphine Lewis bases in catalytic, asymmetric aza-Morita-Baylis-Hillman reaction
Authors: Shi Min , Chen Lian-Hui
Published: December 2005

In the aza-Morita-Baylis-Hillman reaction of N-sulfonated imines with methyl vinyl ketone (MVK) promoted by chiral phosphine Lewis base: (R)-2'-diphenylphosphanyl-[1,1']binaphthalenyl-2-ol (LB1) (10 mol %), the aza-Morita-Baylis-Hillman adducts were obtained in good yields with high ee (70-94 % ee) at -30 °C in THF. The scope and limitations of this reaction have been disclosed.
Enantioselective addition of organozinc reagents to carbonyl compounds
Authors: Yus Miguel , Ramón Diego J.
Published: December 2005

Different chiral camphorsulfonamide derivatives containing a hydroxy or a sulfonamido functionality, as well as chiral 1,2-hydroxysulfonamides, have been evaluated as chiral promoters in the classical enantioselective addition of dialkylzinc reagents to aldehydes in the presence of titanium tetraisopropoxide (ee up to 96 %). Surprisingly, ligands with a structure of isoborneol are able to promote the related unknown addition to ketones (ee >99 %), the best ligand being the exo-diol derived from 1,2-trans-biscamphorsulfonamidocyclohexane (HOCSAC).
Supramolecular assemblies with DNA* (Special Topic Article)
Authors: Barthélémy Philippe , Lee Stephen J. , Grinstaff Mark
Published: December 2005

Information storage in chemical and biological systems involves recognition processes occurring at the molecular and macromolecular level. The implementation of a "code" can consist of multiple noncovalent interactions, which include hydrogen bonds, π-stacking, hydrophobic interactions, and appropriate molecular and supramolecular architectures. With the double-helical DNA structure stabilized by Watson-Crick hydrogen bond base-pairing and aryl π-π stacking interactions, nature provides to scientists an example of one of the most sophisticated supramolecular systems. Molecular organization using these types of processes has become a very powerful strategy for the construction of well-defined nanostructures. Self-assemblies using noncovalent interactions have been designed to build fibers, membranes, two-dimensional monolayers, hydro, organo gels, etc. This paper highlights the research presented at the workshop entitled DNA Supramolecular Assemblies, which was held in Avignon, France on 5-6 May 2004. In this article, we first focus on the recent progress achieved in the design of supramolecular self-assemblies that mimic the molecular recognition functionalities found with nucleic acids. Second, we present several synthetic-DNA supramolecular assemblies currently developed to transport nucleic acids into cells. The marriage of supramolecular chemistry with nucleic acids as illustrated through examples in this article will open new avenues for designing artificial molecular devices and expand the current repertoire of supramolecular assemblies available.
Atomic force microscopy and direct surface force measurements (IUPAC Technical Report)
Authors: Ralston John , Larson Ian , Rutland Mark W. , Feiler Adam A. , Kleijn Mieke
Published: December 2005

The atomic force microscope (AFM) is designed to provide high-resolution (in the ideal case, atomic) topographical analysis, applicable to both conducting and nonconducting surfaces. The basic imaging principle is very simple: a sample attached to a piezoelectric positioner is rastered beneath a sharp tip attached to a sensitive cantilever spring. Undulations in the surface lead to deflection of the spring, which is monitored optically. Usually, a feedback loop is employed, which holds the spring deflection constant, and the corresponding movement of the piezoelectric positioner thus generates the image. From this it can be seen that the scanning AFM has all the attributes necessary for the determination of surface and adhesion forces; a sensitive spring to determine the force, a piezoelectric crystal to alter the separation of the tip and surface, which if sufficiently well-calibrated also allows the relative separation of the tip and surface to be calculated. One can routinely quantify both the net surface force (and its separation dependence) as the probe approaches the sample, and any adhesion (pull-off) force on retraction. Interactions in relevant or practical systems may be studied, and, in such cases, a distinct advantage of the AFM technique is that a particle of interest can be attached to the end of the cantilever and the interaction with a sample of choice can be studied, a method often referred to as colloid probe microscopy. The AFM, or, more correctly, the scanning probe microscope, can thus be used to measure surface and frictional forces, the two foci of this article. There have been a wealth of force and friction measurements performed between an AFM tip and a surface, and many of the calibration and analysis issues are identical to those necessary for colloid probe work. We emphasize that this article confines itself primarily to elements of colloid probe measurement using the AFM.
Archaeology and the “Savage Slot”: Displacement and Emplacement in the Premodern World
Authors: COBB CHARLES R.
Published: December 2005

Many attempts to understand the cultural impact of the forces of modernism, capitalism, and globalization have come to highlight contemporary cultural diversity at the expense of reifying a homogenized past of traditional, static societies. The “savage slot” still provides a convenient myth for characterizing small‐scale communities before the advent of modernism—communities that experienced dramatic change only as they were pulled into the world system. Archaeological evidence from the southeastern United States challenges this stereotype, as Native American groups routinely migrated and continually redefined notions of “place” and “locality”—processes often treated as distinctly (post)modern. Such case studies emphasize the importance of working toward a deep historical anthropology that will continue to undermine stereotypes about the Other in the past as well as the present.
Between Religion and Desire: Being Muslim and Gay in Indonesia
Authors: BOELLSTORFF TOM
Published: December 2005

Thousands of Indonesian men now identify as both “gay” and “Muslim.” How do these men understand the relationship between religion and sexuality? How do these understandings reflect the fact that they live in the nation that is home to more Muslims than any other? In this article, I address questions such as these through an ethnographic study of gay Muslims. I argue that dominant social norms render being gay and being Muslim “ungrammatical” with each other in the public sphere that is crucial to Muslim life in Indonesia. Through examining doctrine, interpretation, and community, I explore how gay Muslim subjectivity takes form in this incommensurability between religion and desire.
The Truth from the Body: Medical Certificates as Ultimate Evidence for Asylum Seekers
Authors: FASSIN DIDIER , D'HALLUIN ESTELLE
Published: December 2005

Whether through traditional law or modern torture, the body has always been a privileged site on which to demonstrate the evidence of power. But for immigrants, the poor, and, more generally, the dominated—all of whom have to prove their eligibility to certain social rights—it has also become the place that displays the evidence of truth. In France, as immigration control increases, asylum seekers are more and more submitted to the evaluation of their physical sequels and psychic traumas, as if their autobiographical accounts were not sufficient. In this article, we show how nongovernmental organizations (NGOs) deal with the dilemmas posed by this situation, how they develop protocols standardizing their expertise, and how their medical authority progressively substitutes itself for the asylum seekers' word. In this process of objectification, it is the experience of the victims as political subjects that is progressively erased.
Explaining Corporal Punishment of Children: A Cross‐Cultural Study
Authors: EMBER CAROL R. , EMBER MELVIN
Published: December 2005

Corporal punishment of children is a frequent child‐training technique in many societies in the ethnographic record. In other societies it is infrequent or rare. Using a worldwide sample of largely preindustrial societies in this article, we test previous and new theories that might explain the variation. Our multiple regression analyses indicate that frequent corporal punishment of children is predicted by higher levels of social stratification and political integration, and long‐term use of an alien currency. These findings are consistent with our theory that societies are likely to practice corporal punishment to prepare children for living in a society with native or imposed (e.g., colonial) power inequality. In addition, corporal punishment appears more likely in societies in which nonrelative caretakers help raise children. And in nonpacified societies, undemocratic political decision making and a culture of violence also predict corporal punishment of children.
The Politics of Mediation: Local–Global Interactions in the Central Amazon of Brazil
Authors: CHERNELA JANET
Published: December 2005

The proliferation of a nongovernmental sector held the promise of linking local actors with national and international ones, thereby contributing to a highly participatory, Habermasian ideal in which the formerly marginalized would find greater participation and expression. Yet the role of international agents in community‐based resource management projects has recently come under scrutiny. In addressing these issues in this article, I consider the roles of different interlocutors in two contrastive phases in an Amazonian community's movement to preserve its endangered fisheries. The comparative exercise demonstrates how institutional agents, by establishing a discourse that structures the criteria through which collective demands may be problematized, may inadvertently shift from mediation to domination, and from local partnering to local production.