bluuebunny/crossref_metadata_2025 huggingface dataset:
Combined analysis of metagenome and transcriptome revealed the adaptive mechanism of different golden Camellia species in karst regions
Authors: Liu Jianxiu , Jiang Haidu , Huang Yang , Zhong Lisha , Xu Qin , Yang Quanguang , Liu Shengyuan , Wei Xiao , Liang Yu , Chai Shengfeng
Published: November 2023

Camellia sect. Chrysantha is an important rare and protected plant species. Some golden Camellia species grow in karst soil while others grow in acidic soil. In order to study the adaptation mechanism of golden Camellia to the karst environment, four species of golden Camellia growing in the karst soil (Camellia pubipetala, Camellia perpetua, Camellia grandis, and Camellia limonia) and four species growing in the acidic soil (Camellia nitidissima, Camellia euphlebia, Camellia tunghinensis, and Camellia parvipetala) were selected for this study. Combining the metagenome and transcriptome, the structure and function of the rhizosphere microbial communities and the gene expression in roots of golden Camellia were analyzed. The results showed that the rhizosphere microbial communities in different golden Camellia were significantly different in abundance of Acidobacteria, Actinobacteria, Candidatus_Rokubacteria, Nitrospirae, Planctomycetes, and Candidatus_Tectomicrobia. The proportion of Candidatus_Rokubacteria was significantly higher in the rhizosphere soil of four species of golden Camellia grown in karst areas, compared to C. nitidissima, C. euphlebia, and C. tunghinensis. The linear discriminant analysis Effect Size showed that C. parvipetala was similar to karst species in the enrichment of ABC transporters and quorum sensing. During the transcriptome analysis, numerous upregulated genes in four karst species, including CYP81E, CHS, F3H, C12RT1, NAS, and CAD, were found to be enriched in the secondary metabolite synthesis pathway in the KEGG library, when compared to C. tunghinensis. This study provides information for plant adaptation mechanisms on the rhizosphere soil microbial composition and gene expression in secondary metabolic pathways to karst habitats and its distribution in karst areas.
Changes in fear-associated learning task brain activation over the COVID-19 pandemic period: a preliminary longitudinal analysis
Authors: Popovich Claire , Grau Aaron S. , Shih Chia-Hao , Chidiac Neejad T. , Zhou Adrian , Wang Xin , Xie Hong
Published: November 2023

Background

The COVID-19 pandemic has had profound impacts on people worldwide. Previous studies have shown that fear learning, extinction, recall, and contextual information processing involve the activation of emotion and sensory brain systems, which can be modified. However, it remains unclear whether brain functions associated with these processes have been altered over the pandemic period.

Methods

We compared pre- and peri-pandemic brain activation during a fear-associated learning task (FALT) using previously collected data. The participants were divided into two groups: the pandemic group (n = 16), who completed a baseline FALT before the pandemic and repeated the task during the pandemic, and the non-pandemic group (n = 77), who completed both sessions before the pandemic began.

Results

Compared with the non-pandemic group, the pandemic group exhibited significant decreases in brain activation from baseline to follow-up assessments, including activation in the brainstem during early fear learning, the posterior thalamus/hippocampus during late extinction, and the occipital pole during late recall phases for contextual processing. Furthermore, activations associated with retrieving safety cues were reduced in the posterior cingulate, premotor, and calcarine cortices during the early recall phase, and activations associated with retrieving dangerous cues decreased in the occipital pole during the late recall phase. Additionally, correlations between decreased activation and elevated posttraumatic stress symptoms were observed.

Conclusion

These findings suggest that activations associated with processing low arousal contextual information, safety cues, and extinguished fear cues decreased during the pandemic. These changes in brain activation may have contributed to the increase in mental health disturbances observed during this time.
Biological studies of clavine alkaloids targeting CNS receptors
Authors: Tasker Nikhil R. , Pazur Ethan J. , Wipf Peter
Published: November 2023

In contrast to well established psychedelics such as lysergic acid diethylamide (LSD) and psilocybin, ergot alkaloids of the clavine subclass have not been thoroughly investigated, in spite of their broad occurrence in nature and their well-established potent physiological effects. This study presents the current knowledge on the biological properties of clavine alkaloids, draws comparisons to the pharmacology of ergolines and related psychedelics, and demonstrates opportunities to develop novel structure–activity relationship (SAR) profiles. The latter could usher in a new stage of medicinal chemistry studies that enable an expansion of the currently structurally limited portfolio of psychedelic therapeutics.
A comparison of quality of life between older adults living in high and low altitude areas
Authors: Liu Shou , Wang Fei , Sha Sha , Cai Hong , Ng Chee H. , Feng Yuan , Xiang Yu-Tao
Published: November 2023

Background

High altitude is known to have a significant impact on human physiology and health, therefore, understanding its relationship with quality of life is an important research area. This study compared the quality of life (QOL) in older adults living in high and low altitude areas, and examined the independent correlates of QOL in those living in a high altitude area.

Methods

Older adults living in three public nursing homes in Xining (high altitude area) and one public nursing home in Guangzhou (low altitude area) were recruited. The WHOQOL-BREF was used to measure the QOL.

Results

644 older adults (male: 39.1%) were included, with 207 living in high altitude and 437 living in low altitude areas. After controlling for the covariates, older adults living in the high altitude area had higher QOL in terms of physical (P = 0.035) and social domains (P = 0.002), but had lower QOL in psychological (P = 0.009) domain compared to their counterparts living in the low altitude area. For older adults living in the high altitude area, smoking status was associated with higher social QOL (P = 0.021), good financial status was associated with higher physical QOL (P = 0.035), and fair or good health status was associated with higher physical (p < 0.001) and psychological QOL (P = 0.046), while more severe depressive symptoms were associated with lower QOL.

Conclusion

Appropriate interventions and support to improve depressive symptoms and both financial and health status should be developed for older adults living in high altitude areas to improve their QOL.
Parental anxiety and depression are associated with adverse mental health in children with special needs during the COVID-19 pandemic
Authors: Gruszka Piotr , Ganahl Kristin , Stasch Nicole , Burger Christoph , Haberlandt Edda , Bauer Susanne M.
Published: November 2023

Introduction

The coronavirus disease 2019 (COVID-19) pandemic has led to restrictions in various areas of life, including social life, work, leisure, health, and education. Vulnerable groups, such as children with special needs and their parents, may be at increased risk of experiencing exacerbated mental health problems during stressful periods such as the COVID-19 lockdowns.

Materials and methods

Telephone interviews were conducted with 954 parents of children with special needs. We assessed parental levels of generalized anxiety and depression using the validated GAD-7 and PHQ-8 scales. Parents were asked to rate family burden and their worry about the COVID-19 crisis, as well as their children's adverse mental health symptoms and health behaviors. Parents also reported their children's worries about the COVID-19 crisis. We conducted regressions to examine the relationship between parents' mental health problems and their children's adverse mental health symptoms and health behaviors. Qualitative data from open-ended questions were coded thematically and major themes of parental worry about the COVID-19 crisis were identified.

Results

Parental anxiety and depression symptoms predicted adverse mental health symptoms and behaviors in children with special needs. Criteria for current depression were met by 7.9% of parents of children with special needs, whereas 4.7% of the general population in Vorarlberg met the criteria for current depression according to data from the Austrian Health Interview Survey in 2019. Parental self-ratings of both depression and anxiety were highly correlated. The majority of parents reported being burdened (79.1%) or worried (67.8%) about the COVID-19 crisis. The main themes of parental worry about the COVID-19 crisis included COVID-19 infection (40.6%), economic situation (13.1%), uncertainty (8.4%), lack of social contact with family and friends (8.1%), family health status (7.5%), and school life (7.5%).

Discussion

Mental health symptoms in parents of children with special needs were strongly associated with increased adverse mental health symptoms and health behaviors in their children. Parents of children with special needs were more likely to be depressed during the COVID-19 pandemic than adults in 2019. We call for additional mental health support to reduce the mental health burden in families with children with special needs.
The emergence of carbapenemase-producing Enterobacterales in hospitals: a major challenge for a debilitated healthcare system in Lebanon
Authors: Daaboul Dina , Oueslati Saoussen , Rima Mariam , Kassem Issmat I. , Mallat Hassan , Birer Aurélien , Girlich Delphine , Hamze Monzer , Dabboussi Fouad , Osman Marwan , Naas Thierry
Published: November 2023

Background

Carbapenem- and extended-spectrum cephalosporin-resistant Enterobacterales (CR-E and ESCR-E, respectively) are increasingly isolated worldwide. Information about these bacteria is sporadic in Lebanon and generally relies on conventional diagnostic methods, which is detrimental for a country that is struggling with an unprecedented economic crisis and a collapsing public health system. Here, CR-E isolates from different Lebanese hospitals were characterized.

Materials and methods

Non-duplicate clinical ESCR-E or CR-E isolates (N = 188) were collected from three hospitals from June 2019 to December 2020. Isolates were identified by MALDI-TOF, and their antibiotic susceptibility by Kirby-Bauer disk diffusion assay. CR-E isolates (n = 33/188) were further analyzed using Illumina-based WGS to identify resistome, MLST, and plasmid types. Additionally, the genetic relatedness of the CR-E isolates was evaluated using an Infrared Biotyper system and compared to WGS.

Results

Using the Kirby-Bauer disk diffusion assay, only 90 isolates out of the 188 isolates that were collected based on their initial routine susceptibility profile by the three participating hospitals could be confirmed as ESCR-E or CR-E isolates and were included in this study. This collection comprised E. coli (n = 70; 77.8%), K. pneumoniae (n = 13; 14.4%), Enterobacter spp. (n = 6; 6.7%), and Proteus mirabilis (n = 1; 1.1%). While 57 were only ESBL producers the remaining 33 isolates (i.e., 26 E. coli, five K. pneumoniae, one E. cloacae, and one Enterobacter hormaechei) were resistant to at least one carbapenem, of which 20 were also ESBL-producers. Among the 33 CR-E, five different carbapenemase determinants were identified: blaNDM-5 (14/33), blaOXA-244 (10/33), blaOXA-48 (5/33), blaNDM-1 (3/33), and blaOXA-181 (1/33) genes. Notably, 20 CR-E isolates were also ESBL-producers. The analysis of the genetic relatedness revealed a substantial genetic diversity among CR-E isolates, suggesting evolution and transmission from various sources.

Conclusion

This study highlighted the emergence and broad dissemination of blaNDM-5 and blaOXA-244 genes in Lebanese clinical settings. The weak AMR awareness in the Lebanese community and the ongoing economic and healthcare challenges have spurred self-medication practices. Our findings highlight an urgent need for transformative approaches to combat antimicrobial resistance in both community and hospital settings.
The Association Between Tobacco Use and Risk of COVID-19 Infection and Clinical Outcomes in Sweden: A Population-Based Study
Authors: Shaaban A. N. , Andersson F. , Peña S. , Caspersen I. H. , Magnusson C. , Orsini N. , Karvonen S. , Magnus P. , Hergens M. P. , Galanti M. R.
Published: November 2023

Background: The association between tobacco use and COVID-19 is controversial. During the early course of the pandemic, limited testing prevented studying a wide spectrum of clinical manifestations.

Objective: To examine the potential causal association between tobacco use and COVID-19 during the second wave (1 October 2020–30 June 2021) of the pandemic in Stockholm, Sweden.

Methods: A population-based cohort study was conducted in the Stockholm region of Sweden, with information on tobacco use collected prior to the pandemic. Adjusted relative risks (RR) of COVID-19 and 95% confidence intervals (CI) were calculated, contrasting current smokers and snus users to non-users of tobacco.

Results: Compared with non-users of tobacco, current smokers had a lower risk of COVID-19 (RR 0.78, 95% CI = 0.75–0.81) and of hospitalisation for the disease. Current snus users had a higher risk of COVID-19. Heavy smokers and snus users had longer hospital stays than non-users of tobacco.

Conclusion: Tobacco use may have a different impact on the risk of being infected with SARS-CoV-2 and the risk of developing severe clinical manifestations. Further research is needed to determine the underlying mechanisms.
Advances in Virtual Cutting Guide and Stereotactic Navigation for Complex Tumor Resections of the Sacrum and Pelvis: Case Series with Short-Term Follow-Up
Authors: Hirase Takashi , McChesney Grant R. , Garvin Lawrence , Tappa Karthik , Satcher Robert L. , Mericli Alexander F. , Rhines Laurence D. , Bird Justin E.
Published: November 2023

Primary malignancies of the sacrum and pelvis are aggressive in nature, and achieving negative margins is essential for preventing recurrence and improving survival after en bloc resections. However, these are particularly challenging interventions due to the complex anatomy and proximity to vital structures. Using virtual cutting guides to perform navigated osteotomies may be a reliable method for safely obtaining negative margins in complex tumor resections of the sacrum and pelvis. This study details the technique and presents short-term outcomes. Patients who underwent an en bloc tumor resection of the sacrum and/or pelvis using virtual cutting guides with a minimum follow-up of two years were retrospectively analyzed and included in this study. Preoperative computer-assisted design (CAD) was used to design osteotomies in each case. Segmentation, delineating the tumor from normal tissue, was performed by the senior author using preoperative CT scans and MRI. Working with a team of biomedical engineers, virtual surgical planning was performed to create osteotomy lines on the preoperative CT and overlaid onto the intraoperative CT. The pre-planned osteotomy lines were visualized as “virtual cutting guides” providing real-time stereotactic navigation. A precision ultrasound-powered cutting tool was then integrated into the navigation system and used to perform the osteotomies in each case. Six patients (mean age 52.2 ± 17.7 years, 2 males, 4 females) were included in this study. Negative margins were achieved in all patients with no intraoperative complications. Mean follow-up was 38.0 ± 6.5 months (range, 24.8–42.2). Mean operative time was 1229 min (range, 522–2063). Mean length of stay (LOS) was 18.7 ± 14.5 days. There were no cases of 30-day readmissions, 30-day reoperations, or 2-year mortality. One patient was complicated by flap necrosis, which was successfully treated with irrigation and debridement and primary closure. One patient had local tumor recurrence at final follow-up and two patients are currently undergoing treatment for metastatic disease. Using virtual cutting guides to perform navigated osteotomies is a safe technique that can facilitate complex tumor resections of the sacrum and pelvis.
Tuberculosis Severity Predictive Model Using Mtb Variants and Serum Biomarkers in a Colombian Cohort of APTB Patients
Authors: Ocampo Juan C. , Alzate Juan F. , Barrera Luis F. , Baena Andres
Published: November 2023

Currently, tuberculosis (TB) is a bacterial infection caused by Mycobacterium tuberculosis (Mtb) that primarily affects the lungs. The severity of active pulmonary TB (APTB) is an important determinant of transmission, morbidity, mortality, disease experience, and treatment outcomes. Several publications have shown a high prevalence of disabling complications in individuals who have had severe APTB. Furthermore, certain strains of Mtb were associated with more severe disease outcomes. The use of biomarkers to predict severe APTB patients who are candidates for host-directed therapies, due to the high risk of developing post-tuberculous lung disease (PTLD), has not yet been implemented in the management of TB patients. We followed 108 individuals with APTB for 6 months using clinical tools, flow cytometry, and whole-genome sequencing (WGS). The median age of the study population was 26.5 years, and the frequency of women was 53.7%. In this study, we aimed to identify biomarkers that could help us to recognize individuals with APTB and improve our understanding of the immunopathology in these individuals. In this study, we conducted a follow-up on the treatment progress of 121 cases of APTB. The follow-up process commenced at the time of diagnosis (T0), continued with a control visit at 2 months (T2), and culminated in an exit appointment at 6 months following the completion of medical treatment (T6). People classified with severe APTB showed significantly higher levels of IL-6 (14.7 pg/mL; p < 0.05) compared to those with mild APTB (7.7 pg/mL) at T0. The AUCs for the ROC curves and the Matthews correlation coefficient values (MCC) demonstrate correlations ranging from moderate to very strong. We conducted WGS on 88 clinical isolates of Mtb, and our analysis revealed a total of 325 genes with insertions and deletions (Indels) within their coding regions when compared to the Mtb H37Rv reference genome. The pattern of association was found between serum levels of CHIT1 and the presence of Indels in Mtb isolates from patients with severe APTB. A key finding in our study was the high levels of CHIT1 in severe APTB patients. We identified a biomarker profile (IL-6, IFN-γ, IL-33, and CHIT1) that allows us to identify individuals with severe APTB, as well as the identification of a panel of polymorphisms (125) in clinical isolates of Mtb from individuals with severe APTB. Integrating these findings into a predictive model of severity would show promise for the management of APTB patients in the future, to guide host-directed therapy and reduce the prevalence of PTLD.
A Next-Generation Sequencing Study in a Cohort of Sicilian Patients with Parkinson’s Disease
Authors: Salemi Michele , Lanza Giuseppe , Salluzzo Maria Grazia , Schillaci Francesca A. , Di Blasi Francesco Domenico , Cordella Angela , Caniglia Salvatore , Lanuzza Bartolo , Morreale Manuela , Marano Pietro , Tripodi Mariangela , Ferri Raffaele
Published: November 2023

Parkinson’s disease (PD) is a multisystem and multifactorial disorder and, therefore, the application of modern genetic techniques may assist in unraveling its complex pathophysiology. We conducted a clinical–demographic evaluation of 126 patients with PD, all of whom were Caucasian and of Sicilian ancestry. DNA was extracted from the peripheral blood for each patient, followed by sequencing using a Next-Generation Sequencing system. This system was based on a custom gene panel comprising 162 genes. The sample underwent further filtering, taking into account the allele frequencies of genetic variants, their presence in the Human Gene Mutation Database, and their association in the literature with PD or other movement/neurodegenerative disorders. The largest number of variants was identified in the leucine-rich repeat kinase 2 (LRRK2) gene. However, variants in other genes, such as acid beta-glucosidase (GBA), DNA polymerase gamma catalytic subunit (POLG), and parkin RBR E3 ubiquitin protein ligase (PRKN), were also discovered. Interestingly, some of these variants had not been previously associated with PD. Enhancing our understanding of the genetic basis of PD and identifying new variants possibly linked to the disease will contribute to improved diagnostic accuracy, therapeutic developments, and prognostic insights for affected individuals.
Clinical Tools for Rectal Cancer Response Assessment following Neoadjuvant Treatment in the Era of Organ Preservation
Authors: Stefanou Amalia J. , Dessureault Sophie , Sanchez Julian , Felder Seth
Published: November 2023

Local tumor response evaluation following neoadjuvant treatment(s) in rectal adenocarcinoma requires a multi-modality approach including physical and endoscopic evaluations, rectal protocoled MRI, and cross-sectional imaging. Clinical tumor response exists on a spectrum from complete clinical response (cCR), defined as the absence of clinical evidence of residual tumor, to near-complete response (nCR), which assumes a significant reduction in tumor burden but with increased uncertainty of residual microscopic disease, to incomplete clinical response (iCR), which incorporates all responses less than nCR that is not progressive disease. This article aims to review the clinical tools currently routinely available to evaluate treatment response and offers a potential management approach based on the extent of local tumor response.
“Multisystem Inflammatory Syndrome in Children”-Like Disease after COVID-19 Vaccination (MIS-V) with Potential Significance of Functional Active Autoantibodies Targeting G-Protein-Coupled Receptors (GPCR-fAAb) for Pathophysiology and Therapy
Authors: Schmidt Marius , Hébert Steven , Wallukat Gerd , Ponader Rolf , Krickau Tobias , Galiano Matthias , Reutter Heiko , Woelfle Joachim , Agaimy Abbas , Mardin Christian , Hoerning André , Hohberger Bettina
Published: November 2023

Background: An infection with SARS-CoV-2 can trigger a systemic disorder by pathological autoimmune processes. A certain type of this dysregulation is known as Multisystemic inflammatory syndrome in children (MIS-C). However, similar symptoms may occur and have been described as Multisystemic inflammatory syndrome after SARS-CoV-2 Vaccination (MIS-V) following vaccination against SARS-CoV-2. We report the case of a 12-year-old boy who was identified with MIS-C symptoms without previous SARS-CoV-2 infection after receiving two doses of the Pfizer–BioNTech COVID-19 vaccine approximately one month prior to the onset of symptoms. He showed polyserositis, severe gastrointestinal symptoms and, consequently, a manifestation of a multiorgan failure. IgG antibodies against spike proteins of SARS-CoV-2 were detected, indicating a successful vaccination, while SARS-CoV-2 Nucleocapsid protein antibodies and SARS-CoV-2 PCR were not detected. Several functional, active autoantibodies against G-protein-coupled receptors (GPCR-fAAb), previously associated with Long COVID disease, were detected in a cardiomyocyte bioassay. Immunosuppression with steroids was initiated. Due to side effects, treatment with steroids and later interleukin 1 receptor antagonists had to be terminated. Instead, immunoadsorption was performed and continued with tacrolimus and mycophenolic acid therapy, leading to improvement and discharge after 79 days. GPCR-fAAb decreased during therapy and remained negative after clinical curing and under continued immunosuppressive therapy with tacrolimus and mycophenolic acid. Follow-up of the patient showed him in good condition after one year. Conclusions: Infection with SARS-CoV-2 shows a broad and severe variety of symptoms, partly due to autoimmune dysregulation, which, in some instances, can lead to multiorgan failure. Despite its rarity, post-vaccine MIS-C-like disease may develop into a serious condition triggered by autoimmune dysregulation. The evidence of circulating GPCR-fAAb and their disappearance after therapy suggests a link of GPCR-fAAb to the clinical manifestations. Thus, we hypothesize a potential role of GPCR-fAAb in pathophysiology and their potential importance for the therapy of MIS-C or MIS-V. However, this observation needs further investigation to prove a causative correlation.
Developing Botanical Formulations for Sustainable Cosmetics
Authors: Lima Lonetá Lauro , Bispo-dos-Santos Karina , Trevisan Ingrid Mayara Cavalcante , Rapôso Catarina , Velho Paulo Eduardo Neves Ferreira , Bagatin Ediléia , Rezende Rodrigo Alvarenga , da Silva Jorge Vicente Lopes , Ricci Leonardi Gislaine
Published: November 2023

Interest in clean beauty is rising due to minimalism in the formulation of cosmetics, rational use of water, and fewer chemical additives like preservatives, colorants, surfactants, and artificial fragrances. Green ingredients lead to the development of sustainable formulations with advanced performance and are less aggressive to human health and the environment. Currently, the electrospinning technique is used as a simple one-step manufacturing process to produce nanostructured cosmetics under mild temperature conditions. This study focuses on the utilization of rice bran oil (RBO) in the creation of sustainable nanostructured cosmetics for potential cosmetic and well-being applications. Four sustainable formulations were developed to optimize the creation of nanostructured cosmetics using ethyl cellulose and rice bran oil (RBO). Ethanol absolute and polyvinyl pyrrolidone have been chosen to compose the sustainable formulation due to their biocompatibility and biodegradability. We studied four different RBO concentrations regarding morphology, encapsulation efficiency, biodegradability, and cytotoxicity. Nanostructured cosmetics present biomimetic surfaces, high RBO encapsulation ability, low mass loss at simulated physiologic conditions, and non-cytotoxicity. Therefore, the minimalist sustainable formulation does not contain any toxic solvents and incompatible harmful excipients, was nanostructured using a mild manufacturing process, and obtained high RBO entrapment.
Quantum Communications Feasibility Tests over a UK-Ireland 224 km Undersea Link
Authors: Amies-King Ben , Schatz Karolina P. , Duan Haofan , Biswas Ayan , Bailey Jack , Felvinti Adrian , Winward Jaimes , Dixon Mike , Minder Mariella , Kumar Rupesh , Albosh Sophie , Lucamarini Marco
Published: November 2023

The future quantum internet will leverage existing communication infrastructures, including deployed optical fibre networks, to enable novel applications that outperform current information technology. In this scenario, we perform a feasibility study of quantum communications over an industrial 224 km submarine optical fibre link deployed between Southport in the United Kingdom (UK) and Portrane in the Republic of Ireland (IE). With a characterisation of phase drift, polarisation stability and the arrival time of entangled photons, we demonstrate the suitability of the link to enable international UK–IE quantum communications for the first time.
Decision-Making Algorithm for the Issuance of the Green Bonds by Municipalities in Inter-Municipal Cooperation in Lithuania
Authors: Bužinskė Julija , Stankevičienė Jelena
Published: November 2023

The objective of this study is to establish a decision-making algorithm for issuing green bonds by municipalities in inter-municipal cooperation in Lithuania with a focus on zero-waste projects. The research methodology comprises four parts. The first part of the research methodology corresponds to a literature review that examines the concept of inter-municipal cooperation and its development in different countries. The second part of the research methodology is dedicated to the formulation of the valuation approach for the inter-municipal cooperation possibility and approbation of it in the selected municipalities. The third part of the research methodology covers the development of the decision-making algorithm for issuing green bonds by municipalities in inter-municipal cooperation in Lithuania. The last part of the research methodology summarizes the results of the survey and proposals for its further development. The findings suggest that the selected municipalities for the valuation of inter-municipal cooperation possibility could improve municipal performance for better inter-municipal cooperation results. The results also show that a smaller municipality corresponds to moderate municipal performance, which can be expected in comparison to a larger municipality. The findings of the construction of the decision-making algorithm suggest a 12-step process for the issuance of green bonds in Lithuania. The practical implications of the study are based on the notion that findings can be applied by municipalities willing to issue green bonds, smaller municipalities having challenges finding financial sources, and municipalities in inter-municipal cooperation sharing the same goals. An original aspect of this paper is its study focus on inter-municipal cooperation valuation and the issuance of green bonds in the municipalities of a smaller country which can serve as a reference point for considering the potential issuance of green bonds in municipalities of similar size.
The Impact of 10 Unmalted Alternative Adjuncts on Wort Characteristics
Authors: Laureys David , Baillière Jeroen , Vermeir Pieter , Vanderputten Dana , De Clippeleer Jessika
Published: November 2023

Consumers are more than ever in search of novel and exciting beer choices, and brewers are, therefore, continuously experimenting to adapt their product portfolio. One interesting way to naturally incorporate novel flavors and tastes is by using alternative adjuncts, but this is not always an easy and straightforward process. In this study, a 40% unmalted alternative adjunct (einkorn, emmer, spelt, khorasan, quinoa, amaranth, buckwheat, sorghum, teff, and tritordeum) or reference (barley malt, unmalted barley, and unmalted wheat) was added to 60% barley malt, after which three different laboratory mashing processes (Congress mash, Congress mash with pre-gelatinization of the adjunct, and Evans mash) were performed, and their behavior during mashing and the resulting wort characteristics were investigated in detail. Overall, the extraction process of all 10 unmalted alternative adjuncts was not complete for all three laboratory mashing processes, whereby Congress mashing resulted in the highest extract and fastest filtration, whereas Evans mashing resulted in the lowest extract and slowest filtration. Pre-gelatinization of the unmalted was generally only beneficial for adjuncts with high onset starch gelatinization temperatures. This process also inactivated endogenous enzymes in the unmalted adjuncts, which had an adverse effect on the mashing process.
The Effects of Structure and Oxidative Polymerization on Antioxidant Activity of Catechins and Polymers
Authors: Wang Wei , Le Ting , Wang Wei-Wei , Yin Jun-Feng , Jiang He-Yuan
Published: November 2023

Polyphenols are key free radical scavengers in tea. This study screened the antioxidant active groups of catechins and dimers and analyzed the effects of the degree of oxidative polymerization and oxidative dimerization reaction on their antioxidant activities. ABTS+· free radical scavenging activity, DPPH free radical scavenging activity, and total antioxidant capacity of catechins and polymers were systematically analyzed and compared in this study. Results manifested antioxidant activities of catechins were dominated by B-ring pyrogallol and 3-galloyl, but were not decided by geometrical isomerism. 3-galloyl had a stronger antioxidant activity than B-ring pyrogallol in catechins. The number, not the position, of the galloyl group was positively correlated with the antioxidant activities of theaflavins. Theasinensin A has more active groups than (−)-epigallocatechin gallate and theaflavin-3,3′-digallate, so it had a stronger antioxidant activity. Additionally, the higher the degree of oxidation polymerization, the weaker the antioxidant activities of the samples. The oxidative dimerization reaction hindered the antioxidant activities of the substrate–catechin mixture by reducing the number of active groups of the substrate and increasing the molecular structure size of the product. Overall, pyrogallol and galloyl groups were antioxidant active groups. The degree of oxidative polymerization and the oxidative dimerization reaction weakened the antioxidant activity.
The Identification and Cytotoxic Evaluation of Nutmeg (Myristica fragrans Houtt.) and Its Substituents
Authors: Khamnuan Suthiwat , Phrutivorapongkul Ampai , Pitchakarn Pornsiri , Buacheen Pensiri , Karinchai Jirarat , Chittasupho Chuda , Na Takuathung Mingkwan , Theansungnoen Tinnakorn , Thongkhao Kannika , Intharuksa Aekkhaluck
Published: November 2023

The aril and seed of nutmeg, Myristica fragrans Houtt. (Myristicaceae), hold significant value in various industries globally. Our preliminary research found two morphological variations: a globose shape and an oval shape. Due to these different characteristics, the safety of consumers is of primary concern. Thus, authentication and comparative pharmacological and toxicity analyses are necessary. In this study, pharmacognostic and advanced phytochemical analyses, DNA barcoding, cytotoxicity, and the anti-nitric oxide production of commercial Thai nutmeg were examined. Via morphologic examinations and TLC fingerprinting, all the sampled aril and seed were categorized into globose and oval-shaped groups. The results of HPLC, GC-MS, and LC-MS/MS experiments revealed distinct differences between these groups. The DNA barcoding of the trnH-psbA region using the BLAST method and neighbor-joining tree analyses confirmed the globose nutmeg as M. fragrans and the oval-shaped variant as M. argentea. A comparison was then carried out between the potential toxicity and anti-inflammatory capabilities of M. fragrans and M. argentea. Cytotoxicity tests on HaCaT, 3T3-L1, Caco-2, HEK293, and RAW264.7 were performed using both methanolic extracts and volatile oil from the arils and seeds of both species. This study concludes that blending or substituting these two species maintains their therapeutic integrity without posing safety concerns.
Embracing Tradition: The Vital Role of Traditional Foods in Achieving Nutrition Security
Authors: Ghosh Sampat , Meyer-Rochow Victor Benno , Jung Chuleui
Published: November 2023

In a press release from the Convention on Biological Diversity (CBD) in 2019, it was reported that, currently, a mere 12 plants and 5 animal species account for approximately 75% of global food production [...]
Characterization and Evaluation of Heat–Moisture-Modified Black and Red Rice Starch: Physicochemical, Microstructural, and Functional Properties
Authors: Ribeiro Victor Herbert de Alcântara , Cavalcanti-Mata Mario Eduardo Rangel Moreira , Almeida Raphael Lucas Jacinto , Silva Virgínia Mirtes de Alcântara
Published: November 2023

This study sought to evaluate starch from black and red rice modified by heat–moisture, investigating the extraction yield, starch and amylose content, color, and phenolic compounds. The water and oil absorption capacity, whole milk and zero lactose absorption index, syneresis index, and texture were also analyzed. Microstructural analysis included Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy. The heat–moisture treatment (HMT) reduced the extraction yield and the starch and amylose content, with native black rice starch having the highest values for these parameters. The modification also affected the color and phenolic compounds of the starch, making it darker and changing its appearance. The modification improved the absorption of water, oil, and milk, reducing syneresis and increasing stability during storage. The starch surface was altered, especially for modified black rice starch, with larger agglomerates. The type of starch also changed from A to Vh, with lower relative crystallinity. The textural properties of modified red rice starch were also significantly altered. The HMT proved to be a viable and economical option to modify the analyzed parameters, influencing the texture and physicochemical properties of pigmented rice starch, expanding its applications, and improving its stability during storage at temperatures above 100 °C.
Differences in Changes in Game Usage Time and Game Use-Related Factors Depending on Parity in a Prospective Study of Pregnant Women in Japan
Authors: Sato Hiroko , Yasui Toshiyuki
Published: November 2023

Game usage has recently been increasing, but the actual situation of game usage and issues among pregnant women are not clarified. The purpose of this prospective longitudinal study was to examine changes in game usage, lifestyle, and thoughts about game usage during pregnancy depending on parity and to clarify the characteristics of pregnant women who continue to use games. We conducted three web surveys in early, mid- and late pregnancy in 238 pregnant women. For primiparous women who continued to use games, there was a significant increase in game usage time from early to late pregnancy (p = 0.022), and 25.0% of those women had anxiety that they might have a game addiction. For primiparous women in mid-pregnancy and multiparous women in early and late pregnancy, the proportions of women who thought that they could not use gaming sufficiently due to pregnancy and child-rearing were significantly higher in women who continued to use games. In both primiparous women and multiparous women, the proportion of partners who used games was significantly higher in women who continued to use games. It is necessary for midwives to discuss with pregnant women and their partners about game usage and to provide advice about control of game usage in daily life.
Preventing Healthcare-Associated Infections: Hand Disinfection Monitoring Using an Automated System in an Italian Neurological Hospital
Authors: Bolcato Vittorio , Robustelli della Cuna Francesco Saverio , Fassina Giovanni , Odone Anna , Gervasio Luisa , Bosone Daniele , Blandi Lorenzo
Published: November 2023

Hand hygiene plays a pivotal role in preventing Healthcare-Associated Infections (HAIs). Nevertheless, the quality of hand disinfection techniques remains suboptimal, and the reliability of assessment methodologies is notably lacking. This study aimed to evaluate hand disinfection techniques using an automated system in an Italian research hospital. Hospital employees underwent automated hand-disinfection technique assessment, according to the World Health Organization’s six-step protocol, at a basal time and two further times. Detection was carried out with a scanner that evaluated the effective hand disinfectant coverage through a fluorescent dye present in the hydro-alcoholic gel rub. The hand-hygiene technique of 222 employees was analyzed by HandInScan®. At the initial assessment of hand coverage with the hydro-alcoholic gel, the mean palm coverage was 82.2%, mean back coverage was 66.8%, and overall mean hand coverage was 74.5%. Then, two measurements were performed in June (t1) and December 2022 (t2). The third measurement showed an increase in hand coverage, with a mean palm coverage of 97.2%, a mean back coverage of 91.8%, and a mean hand coverage of 94.5% (p < 0.05). Moreover, the mean coverage of the hand-back was lower than that of the hand-palm at all times (p < 0.001). The automated scanner provided evidence supporting its effectiveness in enhancing hand hygiene among hospital employees. These findings have motivated researchers to conduct long-term studies, given the potential effects on HAI prevention—including their impact on HAI trends.
Reconceptualizing Social and Emotional Competence Assessment in School Settings
Authors: von der Embse Nathaniel , Kilgus Stephen , Oddleifson Carly , Way Jason D. , Welliver Megan
Published: November 2023

The mental health needs of youth are both significant and increasing. Recent advancements have highlighted the need to reduce psychological distress while promoting the development of important social and emotional competencies. Current social and emotional assessment tools are limited in important ways that preclude their widespread use. In the current article, these limitations are discussed. A novel social and emotional learning assessment framework guided by methodological and theoretical innovations is presented. Future research directions and opportunities are discussed.
Blood Serum Stimulates the Virulence Potential of Mucorales through Enhancement in Mitochondrial Oxidative Metabolism and Rhizoferrin Production
Authors: Patiño-Medina José Alberto , Alejandre-Castañeda Viridiana , Valle-Maldonado Marco Iván , Martínez-Pacheco Mauro Manuel , Ruiz-Herrera León Francisco , Ramírez-Emiliano Joel , Ramírez-Marroquín Oscar Abelardo , Castro-Cerritos Karla Viridiana , Campos-García Jesús , Ramírez-Díaz Martha Isela , Garre Victoriano , Binder Ulrike , Meza-Carmen Víctor
Published: November 2023

This study analyzed the role of blood serum in enhancing the mitochondrial metabolism and virulence of Mucorales through rhizoferrin secretion. We observed that the spores of clinically relevant Mucorales produced in the presence of serum exhibited higher virulence in a heterologous infection model of Galleria mellonella. Cell-free supernatants of the culture broth obtained from spores produced in serum showed increased toxicity against Caenorhabditis elegans, which was linked with the enhanced secretion of rhizoferrin. Spores from Mucoralean species produced or germinated in serum showed increased respiration rates and reactive oxygen species levels. The addition of non-lethal concentrations of potassium cyanide and N-acetylcysteine during the aerobic or anaerobic growth of Mucorales decreased the toxicity of the cell-free supernatants of the culture broth, suggesting that mitochondrial metabolism is important for serum-induced virulence. In support of this hypothesis, a mutant strain of Mucor lusitanicus that lacks fermentation and solely relies on oxidative metabolism exhibited virulence levels comparable to those of the wild-type strain under serum-induced conditions. Contrary to the lower virulence observed, even in the serum, the ADP-ribosylation factor-like 2 deletion strain exhibited decreased mitochondrial activity. Moreover, spores produced in the serum of M. lusitanicus and Rhizopus arrhizus that grew in the presence of a mitophagy inducer showed low virulence. These results suggest that serum-induced mitochondrial activity increases rhizoferrin levels, making Mucorales more virulent.
Research on Belt Deviation Fault Detection Technology of Belt Conveyors Based on Machine Vision
Authors: Wu Xiangfan , Wang Chusen , Tian Zuzhi , Huang Xiankang , Wang Qian
Published: November 2023

Traditional belt deflection detection devices for underground belt conveyors in coal mines have problems, such as their single function, poor fault location and analysis accuracy, low automation level, and low reliability. In order to solve the defects of traditional detection devices, the belt deviation faults of the underground belt conveyor transport process require to be detected effectively and reliably. This paper proposes a belt deviation detection method based on machine vision. This method makes use of a global adaptive high dynamic range imaging method to complete the brightness enhancement processing of the underground image. Then the straight-line features of the conveyor belt edges are extracted using Canny edge detection and the Hough transform algorithm. In addition, a dual-baseline localization judgment method is proposed to realize the identification of band bias faults. Finally, a test bench for belt conveyor deviation was built. Testing experiments for different deviations were conducted. The accuracy of the tape deviation detection reached 99.45%. The method proposed in this study improves the reliability of belt deviation fault detection of underground belt conveyors in coal mines and has wide application prospects in the field of coal mining.
An Augmented Reality-Assisted Disassembly Approach for End-of-Life Vehicle Power Batteries
Authors: Li Jie , Liu Bo , Duan Liangliang , Bao Jinsong
Published: November 2023

The rapid expansion of the global electric vehicle industry has presented significant challenges in the management of end-of-life power batteries. Retired power batteries contain valuable resources, such as lithium, cobalt, nickel, and other metals, which can be recycled and reused in various applications. The existing disassembly processes rely on manual operations that are time-consuming, labour-intensive, and prone to errors. This research proposes an intelligent augmented reality (AR)-assisted disassembly approach that aims to increase disassembly efficiency by providing scene awareness and visual guidance to operators in real-time. The approach starts by employing a deep learning-based instance segmentation method to process the Red-Green-Blue-Dept (RGB-D) data of the disassembly scene. The segmentation method segments the disassembly object instances and reconstructs their point cloud representation, given the corresponding depth information obtained from the instance masks. In addition, to estimate the pose of the disassembly target in the scene and assess their disassembly status, an iterative closed point algorithm is used to align the segmented point cloud instances with the actual disassembly objects. The acquired information is then utilised for the generation of AR instructions, decreasing the need for frequent user interaction during the disassembly processes. To verify the feasibility of the AR-assisted disassembly system, experiments were conducted on end-of-life vehicle power batteries. The results demonstrated that this approach significantly enhanced disassembly efficiency and decreased the frequency of disassembly errors. Consequently, the findings indicate that the proposed approach is effective and holds promise for large-scale industrial recycling and disassembly operations.
A Data-Driven Convolutional Neural Network Approach for Power Quality Disturbance Signal Classification (DeepPQDS-FKTNet)
Authors: Saeed Fahman , Aldera Sultan , Alkhatib Mohammad , Al-Shamma’a Abdullrahman A. , Hussein Farh Hassan M.
Published: November 2023

Power quality disturbance (PQD) signal classification is crucial for the real-time monitoring of modern power grids, assuring safe and reliable operation and user safety. Traditional power quality disturbance signal classification approaches are sensitive to noise, feature selection, etc. This study introduces a novel approach utilizing a data-driven convolutional neural network (CNN) to improve the effectiveness of power quality disturbance signal classification. Deep learning has been successfully used in various fields of recognition, yielding promising outcomes. Deep learning is often characterized as a complex system, with its filters and layers being determined through empirical investigations. A deep learning model was developed for the purpose of classifying PQDs, with the aim of narrowing down the search for unidentified PQDs to a specific problem domain. This approach demonstrates a high level of efficiency in accelerating the process of recognizing PQDs among a vast database of PQDs. In order to automatically identify the number of filters and the number of layers in the model in a PQD dataset, the proposed model uses pyramidal clustering, the Fukunaga–Koontz transform, and the ratio of the between-class scatter to the within-class scatter. The suggested model was assessed using the synthetic dataset generated, with and without the presence of noise. The proposed models outperformed both well-known pre-trained models and state-of-the-art PQD classification techniques in terms of classification accuracy.