bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantifying multi-regional indirect economic losses: An assessment based on the 2021 rainstorm events in China
Authors: Liu Yuan , Li Ying , Wang Guofu , Gao Ge , Chen Yixiao
Published: January 2023

Quantitative assessment of economic losses from disasters can benefit government decision-making as well as mitigation and adaptation strategies. Here, we identified significant rainstorm events in China in 2021 using an objective identification method and investigated the direct economic losses (DELs) from each event. Then, a loss assessment model was developed to estimate the indirect economic losses (IDELs) from rainstorm events. We found that, in 2021, China experienced 36 major rainstorm events, causing approximately 179.8 billion yuan in DELs. The north of China was severely affected by rainstorms and floods, with Henan, Hebei and Shaanxi being the main loss centers. The assessment of IDELs based on rainstorm events showed a non-linear relationship between direct and indirect losses. The socio-economic impact of the 2021 Henan flood (Event No. 15) was the most serious, with direct and indirect losses of 125.8 billion yuan and 269.1 billion yuan, respectively. The primary industry in Henan was seriously affected, and the impact also spread to Inner Mongolia and Guangdong, causing indirect losses of 23.9 billion and 13.1 billion yuan, respectively. We recommend that the indirect losses resulting from such interregional trade linkages should be considered in catastrophe risk management. Finally, the sensitivity analysis showed that moderate overproduction can reduce the indirect impacts caused by disasters. A more detailed study is required to explore how to determine the appropriate levels of disaster relief, as well as a rational funding allocation mechanism.
Present-day activity and seismic potential of the north Qinling fault, southern ordos block, central China, as revealed from GPS data and seismicity
Authors: Yang Chenyi , Ji Lingyun , Yang Yihai , Su Lina , Wang Ying , Shi Huiren
Published: January 2023

The North Qinling Fault, located at the boundary of the North China Block and the South China Block, represents an important tectonic structure between the Weihe Basin and the Qinling Mountains, and controls the subsidence and expansion of the Weihe Basin. This fault has been highly active and has caused strong earthquakes since the Holocene and in a pre-seismic stage currently, as indicated by the many paleoearthquake traces found along it. To determine the present-day activity and seismic potential of the North Qinling Fault, by inverting GPS data, we produced fault locking depth, slip rate, and regional strain fields maps; moreover, based on seismicity, we produced a seismic b-value map. Combining this information with modern seismicity, we were able to comprehensively analyze the seismic potential of different fault segments. Our inversion of GPS data showed that the slip rate of the western segment of the fault (Qingjiangkou–Xitangyu) and the correspondent locking depth are 1.33 mm/a and 13.54 km, respectively, while the slip rate of the middle segment (Xitangyu–Fengyukou) and the correspondent locking depth are 0.45 mm/a and 8.58 km, respectively; finally, the slip rate of the eastern segment (Xitangyu–Daiyu) and the correspondent locking depth are 0.36 mm/a and 21.46 km, respectively. The locking depths of the western and middle segments of the fault are shallower than 90% of the seismic cutoff depth, while the locking depth of the eastern segment of the fault is similar to 90% of the seismic cutoff depth, indicating that “deep creep” occurs in the western and middle segments, while the eastern segment is locked. Modern small earthquakes have involved the western and middle segments of the fault, while the eastern segment has acted as a seismic gap with weak seismicity, characterized by a higher shear strain value and a lower b-value. These characteristics reflect the relationship between the locking depth and seismicity distribution. The results of our comprehensive analysis, combined with field geological surveys, show that the eastern segment of the North Qinling Fault has a strong seismic potential and is presently locked.
Overview of the application of ecological concrete in sponge city construction
Authors: Wang Lei , Liu Zhikui , Yan Yan , Li Shengdong , Xie Yongxiong
Published: January 2023

As a prominent component of the construction field of sponge cities, ecological concrete is an essential tool to reach the goals of green, low-carbon living and sustainable development. A quantitative summary of the preliminary research on ecological concrete infrastructure in sponge city architecture is needed. Therefore, CiteSpace and VOSviewer were applied to perform a comparative analysis of the number of papers, countries, institutions, core authors, literature co-citations, research hotspots, and future trends in ecological concrete in the sponge city construction industry. The results show that the number of papers on ecological concrete is increasing, the research collaboration between domestic and foreign authors is relatively single, and there is insufficient interdisciplinary integration between institutions and the phenomenon of “relatively independent research.” The number of papers published in the field of ecological concrete construction has been on the rise, reaching more than 100 in each of the last 10 years, with China and the United States contributing more to the scientific output of the field. To meet the needs of global environmental protection and resource conservation, the theme of “promoting comprehensive resource conservation and recycling” will continue in the future, making concrete a feature of green, low-carbon, sustainable development and other areas of environmental protection in the construction field.
Risk analysis and countermeasures of TBM tunnelling over the operational tunnel
Authors: Lu Feng , Li Linlong , Chen Zhenyu , Liu Maoyi , Li Pinpin , Gao Xinqiang , Ji Changjun , Gong Lun
Published: January 2023

To study the risk and control countermeasures of the TBM tunnel construction adjacent to the operational railway tunnel, based on the TBM tunnel project of Chongqing Rail Transit Line 5, this paper first evaluates the quality health degree of the operational tunnel lining (OTL) structure according to the on-site structural inspection. Then, the displacement, internal force, and proximity influence scope influenced by the metro TBM tunnel construction are studied using numerical simulation. Finally, the corresponding control countermeasures are proposed. The results show that: (1) The adjacent construction of the upper TBM tunnel will lead to the uplift deformation trend of the lower operational tunnel, and the uplift deformation of the vault is greater than that of the ballast bed. The influence scope is roughly a parallelogram, with the long axis parallel to the operational tunnel and the short axis parallel to the new TBM subway tunnel. (2) TBM tunnelling over the operational tunnel will cause the transformation of the mechanical mode of the OTL structure from the small eccentric compression mode to the large eccentric compression mode. The OTL structure between the left and right lines of TBM is unfavorable. (3) The longitudinal curve of the bending moment and axial force of the OTL fluctuates greatly within the influence range. The bending moment and axial force are reduced in operational tunnel construction joints. Based on field evaluation and numerical analysis, this paper puts forward some risk control countermeasures, such as TBM tunnelling parameters control, pea-gravel backfilling, backfill grouting, and bottom grouting, which can effectively solve the risk of the operational tunnel structure in the adjacent construction. This study has important reference value for risk control and safety assessment of tunnel in complex adjacent tunnel construction.
Controls on anomalously high porosity/permeability of Middle Jurassic deeply buried tight sandstones in the Taibei Sag, Turpan-Hami Basin, northwestern China: Implications for reservoir quality prediction
Authors: Wu Hao , Ban Shuyue , Du Zhiwei , Hao Aisheng , Li Jian , Wei Guoliang , Zhang Hua , Zhang Yunzhao , Wu Heng , Li Mi
Published: January 2023

The lower member of the Middle Jurassic Xishanyao Formation (J2x1+2) sandstones are significant exploratory targets for hydrocarbon resources in the Taibei Sag of the Turpan-Hami Basin, northwestern China. Formation of anomalously high porosity/permeability in deeply buried J2x1+2 sandstones and reservoir quality prediction were investigated using a variety of petrographic analyses. These results show that the J2x1+2 sandstones are mostly feldspathic litharenites and litharenites, which are characterized by moderate-to good sorting, silty to medium granularity, and point- to long grain contacts. The J2x1+2 sandstone has low porosity (avg 6.0%) and permeability (avg 1.12 mD), but shows anomalously high porosity/permeability at depth interval of 3850–4050 m. There is a noticeable variation in pore types and sizes from intergranular-intragranular dissolution pores with a size mode of 2.0 μm in anomalously high porosity (AHP) sandstones, to major intragranular dissolution pores with a size mode of 0.5 μm in normally high porosity (NHP) sandstones, to most micropores with a size mode of 0.25 μm occurred in the normally low porosity (NLP) sandstones. The compaction is the main cause of porosity destruction, resulting in an average porosity loss of 89.6%. However, the authigenic minerals have relatively little influence on reservoir quality. The combination of nappe tectonics and well-sorted particles alleviated the compaction and thus preserved more intergranular pores in the AHP sandstones. Dissolution further increases the porosity and eventually forms the AHP sandstones. The strong carbonate-cemented facies (SCC), tightly compacted facies (TC), moderately compacted with moderate dissolution facies (MCMD), and weakly compacted with strong dissolution facies (WCSD) are determined in the J2x1+2 sandstones. The sandstones with SCC and TC are recognized as the NLP reservoirs. The MCMD and WCSD correspond to the NHP and AHP reservoirs, respectively. The reservoir quality predicted using the constructed diagenetic facies charts is in good agreement with the photomicrograph observations and physical property tests. Additionally, the AHP reservoirs are still developed in the deeply buried sandstones with depths larger than 4000 m.
Evaluation of access and participation plans: Understanding what works
Authors: Moores Elisabeth , Summers Robert J. , Horton Matthew , Woodfield Lizzy , Austen Liz , Crockford Julian
Published: January 2023

We present an analysis of two current policy options to improve evaluation of access and participation work: independent external evaluation vs. in-house evaluation. Evaluation of access and participation work needs to be well-conducted, objective and widely disseminated, regardless of the outcome. Independent external evaluation is likely to provide objectivity and the right skills, but providing effective and timely feedback may be prohibitively expensive. Without support, in-house practitioner teams risk lack of objectivity and skills. Neither external nor in-house evaluation is likely to solve issues of publication bias; usage of open science principles could help. Working with academics and other experts internal to the institution could provide the skills to work well under the open science framework. Working as a sector to avoid duplication of effort is likely to get us further, faster.
Identification of ULK1 as a novel mitophagy-related gene in diabetic nephropathy
Authors: Yang Yuan-Yuan , Gao Zhong-Xiuzi , Mao Zi-Hui , Liu Dong-Wei , Liu Zhang-Suo , Wu Peng
Published: January 2023

Background

Accumulating evidence indicates that mitophagy is crucial for the development of diabetic nephropathy (DN). However, little is known about the key genes involved. The present study is to identify the potential mitophagy-related genes (MRGs) in DN.

Methods

Five datasets were obtained from the Gene Expression Omnibus (GEO) database and were split into the training and validation set. Then the differentially expressed MRGs were screened and further analyzed for GO and KEGG enrichment. Next, three algorithms (SVM-RFE, LASSO and RF) were used to identify hub genes. The ROC curves were plotted based on the hub genes. We then used the CIBERSORT algorithm to assess the infiltration of 22 types of immune cells and explore the correlation between hub genes and immune cells. Finally, the Nephroseq V5 tool was used to analyze the correlation between hub genes and GFR in DN patients.

Results

Compared with the tubulointerstitium, the expression of MRGs was more noticeably varied in the glomeruli. Twelve DE-MRGs were identified in glomerular samples, of which 11 genes were down-regulated and only MFN1 was up-regulated. GO and KEGG analysis indicated that several enrichment terms were associated with changes in autophagy. Three genes (MFN1, ULK1 and PARK2) were finally determined as potential hub genes by three algorithms. In the training set, the AUROC of MFN1, ULK1 and PARK2 were 0.839, 0.906 and 0.842. However, the results of the validation set demonstrated that MFN1 and PARK2 had no significant difference in distinguishing DN samples from healthy controls, while the AUROC of ULK1 was 0.894. Immune infiltration analysis using CIBERSORT showed that ULK1 was positively related to neutrophils, whereas negatively related to M1 and M2 macrophages. Finally, ULK1 was positively correlated with GFR in Nephroseq database.

Conclusions

ULK1 is a potential biomarker for DN and may influence the development of diabetic nephropathy by regulating mitophagy.
Observation study of using a small dose of rituximab treatment for thyroid-associated ophthalmopathy in seven Chinese patients: One pilot study
Authors: Wang Yueyue , Hu Hao , Chen Lu , Zhang Haitao , Yang Tao , Xu Xiaoquan , Chen Huanhuan
Published: January 2023

Objective

To report the efficacy, long-term safety, and tolerability of using a small dose (125 mg/m2 weekly for 4 weeks) of rituximab to treat Chinese patients with thyroid-associated ophthalmopathy (TAO).

Methods

Seven patients with active moderate-to-severe TAO were prospectively recruited in this study. A small dose of rituximab (125mg/m2 body surface area) was given weekly with a duration of four weeks. Thyroid function, thyrotropin receptor antibody (TRAb), B cell and T cell subsets, ophthalmological examination, magnetic resonance imaging derived parameters, and adverse reactions were recorded at each visit.

Results

Seven patients were followed for an average of 224 weeks. B-cell depletion was observed in all patients following rituximab infusion. The clinical activity score (CAS) decreased from 4.86 ± 0.69 to 3.00 ± 0.82 at 5 weeks after treatment (P = 0.033) and remained significantly lower than baseline values at the end of follow-up (P = 0.001). Compared to baseline values, significant decreases in exophthalmos of the right eye, the thickness of extraocular muscles with maximum signal intensity, and the highest signal intensity ratio (SIR) of extraocular muscle to ipsilateral temporal muscle values were observed at the last follow-up (all P < 0.05). Disease progressions or recurrences were not observed during follow-up. Only mild fatigue was observed after the first infusion as a side effect (n = 1).

Conclusion

Small dose of rituximab may be a promising option with adequate safety, tolerability, and long-term efficacy for patients with active moderate-to-severe TAO.
Augmentation of IFN-γ by bone marrow derived immune cells in the presence of severe suppression of IFN-γ in gingivae induced by zoledronic acid and denosumab in Hu-BLT mice model of ONJ
Authors: Kaur Kawaljit , Sun Yujie , Kanayama Keiichi , Morinaga Kenzo , Hokugo Akishige , Nishimura Ichiro , Jewett Anahid
Published: January 2023

Introduction

The potential mechanisms governing drug induced osteonecrosis of the jaw (ONJ) is not well understood, and is one of the objectives of this study. Thus, we tested the release of IFN-γ within different immune compartments including bone marrow and gingivae upon treatment with zoledronic acid (ZOL) and denosumab which are known to induce ONJ in susceptible individuals.

Methods

We used humanized-BLT mouse model for the in-vivo studies reported in this paper. To determine the effects of zoledronic acid and denosumab on IFN-γ secretion and NK cell-mediated cytotoxicity; peripheral blood, bone marrow, spleen and gingiva were obtained after the injection of ZOL and denosumab in mice.

Results

Percentages of B cells are much higher in wild-type mice whereas the proportions of immune subsets in humans and reconstituted hu-BLT peripheral-blood are similar. Therefore, hu-BLT mice are preferable model to study human disease, in particular, immune-pathologies induced by ZOL and denosumab. Both agents resulted in a severe suppression of IFN-γ in the gingiva, whereas they heightened the release of IFN-γ and NK cell-mediated cytotoxicity by the BM-derived immune cells. ZOL increased the IFN-γ secretion by the spleen and peripheral blood immune cells, whereas denosumab decreased the release IFN-γ by these cells significantly.

Discussion

ZOL and denosumab may likely suppress IFN-γ secretion in gingiva through different mechanisms. In addition, to the suppression of IFN-γ secretion, denosumab mediated effect could in part be due to the decrease in the bone resorptive function of osteoclasts due to the induction of antibody dependent cellular cytotoxicity and lysis of osteoclasts, whereas ZOL is able to mediate cell death of osteoclasts directly. Suppression of IFN-gamma in gingiva is largely responsible for the inhibition of immune cell function, leading to dysregulated osteoblastic and osteoclastic activities. Restoration of IFN-gamma in the local microenvironment may result in establishment of homeostatic balance in the gingiva and prevention of osteonecrosis of jaw.
A preliminary study understanding the possibility and benefits of solar photovoltaic collector integration with vertical green balconies in building facade reconstruction
Authors: Wu Zhang , Chul-Soo Kim
Published: January 2023

The facades of buildings provide significant potential for photovoltaic panels integration, allowing renewable energy deployment within the built environment. In literature, various options, such as building-integrated photovoltaics, building-integrated photovoltaics-thermal collectors, building-attached photovoltaics, and rooftop photovoltaics, have already been explored. However, this study aimed to develop a new solar photovoltaic collectors’ integration with vertical-green balconies in old high-rise buildings considering the façade reconstruction concepts mainly focusing on the water heating application. The objective of this study is to conduct a preliminary research study investigating such integration possibilities with old buildings considering the façade reconstruction concepts, followed by exploring various benefits. For this, an old high-rise building was rebuilt scientifically and rationally. PHOENICS tool was used to gauge and assess the building’s wind environment first, followed by the solar photovoltaic collector-based facade installation and preliminary assessment. The results include the system design, economic analysis of the solar photovoltaic collector’s application for water heating, calculation of the energy-saving rate, and functional analysis of the solar photovoltaic collectors combined with a vertical green balcony. The outcome of this study suggested that the process-specific rationalization plan can be applied in future urban architecture renovation.
Technology opportunity discovery of proton exchange membrane fuel cell from the perspective of papers-patents correlation analysis
Authors: Feng Lijie , Liu Kehui , Wang Jinfeng , Lin Kuo-Yi , Zhang Ke , Zhang Luyao
Published: January 2023

Papers and patents can respectively present the latest progress of scientific research and technological development. Combining the two for correlation analysis has certain reference significance for technology opportunity discovery. Therefore, this paper proposes a method for technology opportunity discovery of proton exchange membrane fuel cell from the perspective of papers-patents correlation analysis. Firstly, papers and patent data are collected from papers and patent databases and the LDA model is applied to extract paper and patent topics. Secondly, the indicators of topic heat and novelty are calculated to select high-value paper topics. After that, the similarity analysis between high-value paper topics and all patent topics is considered to select the patent topics with development potential. Finally, the patent texts with high relevance to these patent topics with development potential are positioned. The technical keywords in them are extracted by text mining tool and classified into innovation dimensions according to their attributes and the TEMPEST model. After that, the innovation rules in the SCAMPER model are introduced to combine with the technical keywords in each innovation dimension to identify specific technology opportunities. An empirical study on the technology of proton exchange membrane fuel cell provides sufficient evidence of the method’s ability to discover technology opportunities. This paper contributes by generating four specific technology opportunities, which can make up for the existing defects of proton exchange membrane fuel cells and provide useful guidance for companies to carry out technological innovation.
Parallel Jacobian-free Newton Krylov discrete ordinates method for pin-by-pin neutron transport models
Authors: Zhang Yangyi , Zhou Xiafeng
Published: January 2023

A parallel Jacobian-Free Newton Krylov discrete ordinates method (comePSn_JFNK) is proposed to solve the multi-dimensional multi-group pin-by-pin neutron transport models, which makes full use of the good efficiency and parallel performance of the JFNK framework and the high accuracy of the Sn method for the large-scale models. In this paper, the k-eigenvalue and the scalar fluxes (rather than the angular fluxes) are chosen as the global solution variables of the parallel JFNK method, and the corresponding residual functions are evaluated by the Koch–Baker–Alcouffe (KBA) algorithm with the spatial domain decomposition in the parallel Sn framework. Unlike the original Sn iterative strategy, only a “flattened” power iterative process which includes a single outer iteration without nested inner iterations is required for the JFNK strategy. Finally, the comePSn_JFNK code is developed in C++ language and, the numerical solutions of the 2-D/3-D KAIST-3A benchmark problems and the 2-D/3-D full-core MOX/UOX pin-by-pin models with different control rod distribution show that comePSn_JFNK method can obtain significant efficiency advantage compared with the original power iteration method (comePSn) for the parallel simulation of the large-scale complicated pin-by-pin models.
Can public–private partnership investment in energy (PPPI) mitigate CO2 emissions in South Africa? Fresh evidence from the novel dynamic ARDL simulations approach
Authors: Udeagha Maxwell Chukwudi , Ngepah Nicholas
Published: January 2023

Global warming presents a worrisome condition that necessitates important actions across the planet. One promising strategy is encouraging investments through public–private partnership investment (PPPI) in green ventures. The Environmental Kuznets Curve (EKC) hypothesis framework is used in the current study to examine the relationship between the aforementioned factor and the level of carbon dioxide (CO2) emissions in South Africa between 1960 and 2020. Using the recently developed novel dynamic autoregressive distributed lag (ARDL) simulations framework, the results showed that (i) public–private partnership investment in energy contribute to deteriorating environmental quality; (ii) while the technique effect (TE) improves environmental quality, the scale effect (SE) largely contributes to escalating CO2 emissions, thus confirming that the EKC hypothesis holds; iii) energy consumption, foreign direct investment, trade openness, and industrial growth escalate CO2 emissions, but technological innovation reduces it; and iv) public–private partnership investment in energy, technological innovation, scale effect, technique effect, foreign direct investment, energy consumption, industrial growth, and trade openness Granger-cause CO2 emissions in the short, medium, and long run, showing that these variables are fundamental to determining environmental quality. Our empirical findings suggest that PPPIs should be accomplished in environmentally friendly sectors and industries, and South Africa’s government and policymakers should take further initiatives to ensure the implementation of policies that enable the change from non-renewable energy sources to renewable ones to promote efficiency in the production processes and curtail CO2 emissions in the country.
Spatio-temporal characteristics of PM2.5 and O3 synergic pollutions and influence factors in the Yangtze River Delta
Authors: Zhu Qing , Yu Yang , Gong Haixing , Wang Yanyu , Wang Hongli , Wang Weijie , Xu Bo , Cheng Tiantao
Published: January 2023

Since the implementation of pollution prevention and control action in China in 2013, particulate pollution has been greatly reduced, while ozone pollution has become gradually severe, especially in the economically developed eastern region. Recently, a new situation of air pollution has emerged, namely, enhanced atmospheric oxidation, ascending regional ozone pollution, and increasing particle and ozone synergic pollution (i.e., double-high pollution). Based on the long-term observation data from 2015 to 2021, we examined the spatio-temporal characteristics of urban PM2.5 and O3 pollution in the Yangtze River Delta and quantified the effects of meteorological and non-meteorological factors on pollution in four city clusters using stepwise multiple linear regression models. Temporally, PM2.5 decreased gradually year by year while, O3 increased in city clusters. Spatially, PM2.5 declined from northwest to southeast, while O3 decreased from northeast to southwest. Except for southern Zhejiang, other city clusters suffer from complex air pollution at different levels. In general, pollution intensity and frequency vary with city location and time. Single PM2.5 pollution mostly occurred in northern Anhui. Single O3 pollution occurred in central and southern Jiangsu and northern Zhejiang. Synergic pollutions of PM2.5 and O3 mainly occurred in central Jiangsu. The contributions (90%) of non-meteorological factors (e.g., anthropogenic emission) to PM2.5 decrease and O3 increase are far larger than that of meteorological factors (5%). Relative humidity, sea level pressure, and planetary boundary layer height are the most important meteorological factors to drive PM2.5 changes during pollution. Downward solar radiation, total cloud cover, and precipitation are the most important meteorological factors that affect O3 changes during pollution. The results provide insights into particulate and ozone pollution in the Yangtze River Delta and can help policymakers to formulate accurate air pollution prevention and control strategies at urban and city cluster scales in the future.
Runoff regulation and nitrogen and phosphorus removal performance of a bioretention substrate with HDTMA-modified zeolite
Authors: Qin Yifeng , Chen Mingshen , Liu Shuchang , Fang Yunqing , Li Xudong , Qiu Jiangpin
Published: January 2023

As a commonly used material in bioretention substrates, natural zeolite (NZ) provides decent adsorption capacity for cation pollutants and heavy metals, but limited ability to remove anion pollutants. Hexadecyltrimethylammonium bromide (HDTMA)-modified zeolite (MZ) was used as the bioretention substrate material. The performance of the media including runoff reduction, nitrate nitrogen (NO3−-N) removal, ammonium nitrogen (NH4+-N) removal, and total phosphorus (TP) removal was assessed by the column experiment. The effects of different levels of modification, ratio of zeolite in the substrate, and rainfall intensity on media performance were investigated. The results indicate that HDTMA-modified zeolite significantly improves the NO3−-N (up to 38.2 times of NZ) and TP (up to17.5 times of NZ) removal rate of media and slightly increases the NH4+-N (up to 1.5 times of NZ) purification performance of the substrate. Compared with the media with NZ, decline on both runoff volume reduction (maximum decline up to 32.9%) and flow rate reduction (maximum decline up to 29.9%) of the media with MZ were observed. Based on multiple regression analysis, quantitative relationship models between influencing factors and response variables were established (R2 > 0.793), the level of the effect of influencing factors on response variables was investigated, and the interactions between influencing factors were explored. The main effect analysis found that the degree of modification affects NO3−-N and TP removal rate of the substrate the most, and when the amount of HDTMA molecules loaded on the zeolite surface exceeds 0.09meq/g, the modification can no longer improve NO3−-N removal efficiency.
Estimated discharge of microplastics via urban stormwater during individual rain events
Authors: Ross Matthew S. , Loutan Alyssa , Groeneveld Tianna , Molenaar Danielle , Kroetch Kimberly , Bujaczek Taylor , Kolter Sheldon , Moon Sarah , Huynh Alan , Khayam Rosita , Franczak Brian C. , Camm Eric , Arnold Victoria I , Ruecker Norma J
Published: January 2023

Urban stormwater runoff is an important pathway for the introduction of microplastics and other anthropogenic pollutants into aquatic environments. Highly variable concentrations of microplastics have been reported globally in runoff, but knowledge of key factors within urban environments contributing to this variability remains limited. Furthermore, few studies to date have quantitatively assessed the release of microplastics to receiving waters via runoff. The objectives of this study were to assess the influence of different catchment characteristics on the type and amount of microplastics in runoff and to provide an estimate of the quantity of microplastics discharged during rain events. Stormwater samples were collected during both dry periods (baseflow) and rain events from 15 locations throughout the city of Calgary, Canada’s fourth largest city. These catchments ranged in size and contained different types of predominant land use. Microplastics were found in all samples, with total concentrations ranging from 0.7 to 200.4 pcs/L (mean = 31.9 pcs/L). Fibers were the most prevalent morphology identified (47.7 ± 33.0%), and the greatest percentage of microplastics were found in the 125–250 µm size range (26.6 ± 22.9%) followed by the 37–125 µm size range (24.0 ± 22.3%). Particles were predominantly black (33.5 ± 33.8%), transparent (22.6 ± 31.3%), or blue (16.0 ± 21.6%). Total concentrations, dominant morphologies, and size distributions of microplastics differed between rain events and baseflow, with smaller particles and higher concentrations being found during rain events. Concentrations did not differ significantly amongst catchments with different land use types, but concentrations were positively correlated with maximum runoff flow rate, catchment size, and the percentage of impervious surface area within a catchment. Combining microplastic concentrations with hydrograph data collected during rain events, we estimated that individual outfalls discharged between 1.9 million to 9.6 billion microplastics to receiving waters per rain event. These results provide further evidence that urban stormwater runoff is a significant pathway for the introduction of microplastics into aquatic environments and suggests that mitigation strategies for microplastic pollution should focus on larger urbanized catchments.
Research on the impact of exchange rates and interest rates on carbon price changes in the context of sustainable development
Authors: Yang Jiajun , Wan Yuzhu , Shen Songdong
Published: January 2023

Carbon emissions are closely linked to a company’s production activities. The amount of carbon allowances a company holds determines its carbon emissions. Changes in carbon prices interact with firms’ production activities, while changes in exchange rates and interest rates have a direct impact on firms’ production structure, willingness to produce and scale of production. Under the existing literature, most scholars have selected only one market to be used to study its influence on the carbon market. In this paper, the exchange rate market and the interest rate market are selected for the study of the impact of both on the carbon market, using the following empirical analysis methods: DCC-GARCH, TVP-VAR and GA-BP neural network. The empirical results show that interest rates are positively linked to carbon prices, while exchange rates are more negatively linked to carbon prices; exchange rates are less affected by macro factors and external shocks, while interest rates are the opposite and very sensitive to macro shocks; under the training and simulation of the neural network, carbon prices can fluctuate drastically under the combined influence of exchange rate and interest rate movements, which can provide appropriate early warning of future price fluctuations in carbon trading. This shows that adjustments to exchange rates and interest rates need to be treated with caution, and appropriate adjustments should be made to keep carbon prices stable; the government should build a mechanism to transform the green development of enterprises, pushing them to save energy and reduce emissions to achieve low-carbon transformational development; the government should not only introduce policies to support low-carbon enterprises, but also improve the national carbon market laws and regulations and price regulation mechanisms.
Discovery of a gene cluster for the biosynthesis of novel cyclic peptide compound, KK-1, in Curvularia clavata
Authors: Yamaguchi Shigenari , Fujioka Tomonori , Yoshimi Akira , Kumagai Toshitaka , Umemura Maiko , Abe Keietsu , Machida Masayuki , Kawai Kiyoshi
Published: January 2023

KK-1, a cyclic depsipeptide with 10 residues produced by a filamentous fungus Curvularia clavata BAUA-2787, is a promising pesticide active compound with high activity against many plant pathogens, especially Botrytis cinerea. As a first step toward the future mass production of KK-1 through synthetic biological approaches, we aimed to identify the genes responsible for the KK-1 biosynthesis. To achieve this, we conducted whole genome sequencing and transcriptome analysis of C. clavata BAUA-2787 to predict the KK-1 biosynthetic gene cluster. We then generated the overexpression and deletion mutants for each cluster gene using our originally developed transformation system for this fungus, and analyzed the KK-1 production and the cluster gene expression levels to confirm their involvement in KK-1 biosynthesis. As a result of these, a region of approximately 71 kb was found, containing 10 open reading frames, which were co-induced during KK-1 production, as a biosynthetic gene cluster. These include kk1B, which encodes nonribosomal peptide synthetase with a domain structure that is consistent with the structural features of KK-1, and kk1F, which encodes a transcription factor. The overexpression of kk1F increased the expression of the entire cluster genes and, consequently, improved KK-1 production, whereas its deletion decreased the expression of the entire cluster genes and almost eliminated KK-1 production, demonstrating that the protein encoded by kk1F regulates the expressions of the other nine cluster genes cooperatively as the pathway-specific transcription factor. Furthermore, the deletion of each cluster gene caused a reduction in KK-1 productivity, indicating that each gene is involved in KK-1 production. The genes kk1A, kk1D, kk1H, and kk1I, which showed a significant decrease in KK-1 productivity due to deletion, were presumed to be directly involved in KK-1 structure formation, including the biosynthesis of the constituent residues. kk1C, kk1E, kk1G, and kk1J, which maintained a certain level of KK-1 productivity despite deletion, were possibly involved in promoting or assisting KK-1 production, such as extracellular transportation and the removal of aberrant units incorporated into the peptide chain.
The new oncogene transmembrane protein 60 is a potential therapeutic target in glioma
Authors: Yang Fengdong , Zhang Xuezhi , Wang Xinzhuang , Xue Yake , Liu Xianzhi
Published: January 2023

Glioma is a malignant tumor with a high fatality rate, originating in the central nervous system. Even after standard treatment, the prognosis remains unsatisfactory, probably due to the lack of effective therapeutic targets. The family of transmembrane proteins (TMEM) is a large family of genes that encode proteins closely related to the malicious behavior of tumors. Thus, it is necessary to explore the molecular and clinical characteristics of newly identified oncogenes, such as transmembrane protein 60 (TMEM60), to develop effective treating options for glioma. We used bioinformatic methods and basic experiments to verify the expression of transmembrane protein 60 in gliomas and its relationship with 1p and 19q (1p19q) status, isocitrate dehydrogenase (IDH) status, patient prognosis, and immune cell infiltration using public databases and clinical samples. In addition, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to detect co-expressed genes. Thus, we inhibited the expression of transmembrane protein 60 to observe the proliferation and activity of glioma LN229 cells. We found transmembrane protein 60 was significantly upregulated in glioma compared with that in normal brain tissue at the mRNA. In the subgroups of World Health Organization high grade, isocitrate dehydrogenase wildtype, 1p and 19q non-codeletion, or isocitrate dehydrogenase wild combined with 1p and 19q non-codeletion, the expression of transmembrane protein 60 increased, and the prognosis of glioma patients worsened. In the transmembrane protein 60 high expression group, infiltration of immune cells and stromal cells in the tumor microenvironment increased, tumor purity decreased, and immune cells and pathways were activated. The immune cells mainly included regulatory T-cell, gamma delta T-cell, macrophages M0, neutrophils, and CD8+ T-cells. Overexpression of co-inhibitory receptors (CTLA4, PDL1 and CD96) may promote the increase of depletion of T-cell, thus losing the anti-tumor function in the transmembrane protein 60 high expression group. Finally, we found that transmembrane protein 60 silencing weakened the viability, proliferation, and colony formation of glioma LN229 cells. This is the 0 report on the abnormally high expression of transmembrane protein 60 in glioma and its related clinical features, such as tumor microenvironment, immune response, tumor heterogeneity, and patient prognosis. We also found that transmembrane protein 60 silencing weakened the proliferation and colony formation of glioma LN229 cells. Thus, the new oncogene transmembrane protein 60 might be an effective therapeutic target for the clinical treatment of glioma.
Whole-genome DNA methylome analysis of different developmental stages of the entomopathogenic fungus Beauveria bassiana NCHU-157 by nanopore sequencing
Authors: Li Yi-Hsuan , Chang Ju-Chun , Yen Ming-Ren , Huang Yu-Feng , Chen Tzu-Han , Chen Li-Hung , Nai Yu-Shin
Published: January 2023

The entomopathogenic fungus (EPF), Beauveria bassiana, is an important and commonly used EPF for microbial control. However, the role of DNA methylation has not been thoroughly studied. Therefore, the whole genomic DNA methylome of one promising EPF isolate, B. bassiana NCHU-157 (Bb-NCHU-157), was investigated by Oxford Nanopore Technologies (ONT). First, the whole genome of Bb-NCHU-157 was sequenced by next-generation sequencing (NGS) and ONT. The genome of Bb-NCHU-157 contains 16 contigs with 34.19 Mb and 50% GC content, which are composed of 10,848 putative protein-coding genes. Two putative DNA methyltransferases (DNMTs) were found, including Dim-2 and C-5 cytosine-specific DNA methylases. Both DNMTs showed higher expression levels in the mycelium stage than in the conidia stage, indicating that development of DNA methylation in Bb-NCHU-157 might occur in the mycelium stage. The global methylation level of the mycelium stage (5 mC = 4.56%, CG = 3.33%, CHG = 0.74%, CHH = 0.49%) was higher than that of the conidial stage (5 mC = 2.99%, CG = 1.99%, CHG = 0.63%, CHH = 0.37%) in both the gene and transposable element (TE) regions. Furthermore, the TE regions showed higher methylation frequencies than the gene regions, especially for CHH site methylation, suggesting regulation of genomic stabilization during mycelium development. In the gene regions, high methylation frequencies were found around the transcription start site (TSS) and transcription end site (TES). Moreover, CG and CHG methylation mainly occur in the promoter and intergenic regions, while CHH methylation occurs in the TE region. Among the methylated regions, 371, 661, and 756 differentially DNA methylated regions (DMRs) were hypermethylated in the mycelium in CG, CHG, and CHH, while only 13 and 7 DMRs were hypomethylated in the mycelium in CHG, and CHH, respectively. Genes located in the DMR shared the GO terms, DNA binding (GO: 0003677), and sequence-specific DNA binding (GO: 0043565) for hypermethylation in the mycelium, suggesting that methylation might regulate gene expression from the initial process. Evaluation of the DNA methylome in Bb-NCHU-157 by ONT provided new insight into this field. These data will be further validated, and epigenetic regulation during the development of B. bassiana will be explored.
Manipulation of metabolic pathways to promote stem-like and memory T cell phenotypes for immunotherapy
Authors: Claiborne Michael D.
Published: January 2023

Utilizing the immune system’s capacity to recognize and kill tumor cells has revolutionized cancer therapy in recent decades. Phenotypic study of antitumor T cells supports the principle that superior tumor control is achieved by cells with more long-lived memory or stem-like properties as compared to terminally differentiated effector cells. In this Mini-Review, we explore recent advances in profiling the different metabolic programs that both generate and define subsets of memory T cells. We additionally discuss new experimental approaches that aim to maximize the durability and sustained antitumor response associated with memory T cells within the unique immunosuppressive conditions of the tumor microenvironment, such as engineered attempts to overcome hypoxia-induced changes in mitochondrial function, the inhibitory effects of tumor metabolites, and exploitation of more recently-defined metabolic pathways controlling T cell memory fate such as glycogen metabolism.
Airway microbiome-immune crosstalk in chronic obstructive pulmonary disease
Authors: Kayongo Alex , Robertson Nicole M. , Siddharthan Trishul , Ntayi Moses Levi , Ndawula Josephine Caren , Sande Obondo J. , Bagaya Bernard S. , Kirenga Bruce , Mayanja-Kizza Harriet , Joloba Moses L. , Forslund Sofia K.
Published: January 2023

Chronic Obstructive Pulmonary Disease (COPD) has significantly contributed to global mortality, with three million deaths reported annually. This impact is expected to increase over the next 40 years, with approximately 5 million people predicted to succumb to COPD-related deaths annually. Immune mechanisms driving disease progression have not been fully elucidated. Airway microbiota have been implicated. However, it is still unclear how changes in the airway microbiome drive persistent immune activation and consequent lung damage. Mechanisms mediating microbiome-immune crosstalk in the airways remain unclear. In this review, we examine how dysbiosis mediates airway inflammation in COPD. We give a detailed account of how airway commensal bacteria interact with the mucosal innate and adaptive immune system to regulate immune responses in healthy or diseased airways. Immune-phenotyping airway microbiota could advance COPD immunotherapeutics and identify key open questions that future research must address to further such translation.
Targeting STING: From antiviral immunity to treat osteoporosis
Authors: Gao Zhonghua , Gao Zhongguo , Zhang Hao , Hou Shoubo , Zhou Yunhua , Liu Xiangjie
Published: January 2023

The cGAS-STING signaling pathway can trigger innate immune responses by detecting dsDNA from outside or within the host. In addition, the cGAS-STING signaling pathway has emerged as a critical mediator of the inflammatory response and a new target for inflammatory diseases. STING activation leads to dimerization and translocation to the endoplasmic reticulum Golgi intermediate compartment or Golgi apparatus catalyzed by TBK1, triggers the production of IRF3 and NF-κB and translocates to the nucleus to induce a subsequent interferon response and pro-inflammatory factor production. Osteoporosis is a degenerative bone metabolic disease accompanied by chronic sterile inflammation. Activating the STING/IFN-β signaling pathway can reduce bone resorption by inhibiting osteoclast differentiation. Conversely, activation of STING/NF-κB leads to the formation of osteoporosis by increasing bone resorption and decreasing bone formation. In addition, activation of STING inhibits the generation of type H vessels with the capacity to osteogenesis, thereby inhibiting bone formation. Here, we outline the mechanism of action of STING and its downstream in osteoporosis and discuss the role of targeting STING in the treatment of osteoporosis, thus providing new ideas for the treatment of osteoporosis.
Vesicular traffic-mediated cell-to-cell signaling at the immune synapse in Ankylosing Spondylitis
Authors: Tavasolian Fataneh , Pastrello Chiara , Ahmed Zuhaib , Jurisica Igor , Inman Robert D.
Published: January 2023

The chronic inflammatory disease ankylosing spondylitis (AS) is marked by back discomfort, spinal ankylosis, and extra-articular symptoms. In AS, inflammation is responsible for both pain and spinal ankylosis. However, the processes that sustain chronic inflammation remain unknown. Despite the years of research conducted to decipher the intricacy of AS, little progress has been made in identifying the signaling events that lead to the development of this disease. T cells, an immune cell type that initiates and regulates the body’s response to infection, have been established to substantially impact the development of AS. T lymphocytes are regarded as a crucial part of adaptive immunity for the control of the immune system. A highly coordinated interaction involving antigen-presenting cells (APCs) and T cells that regulate T cell activation constitutes an immunological synapse (IS). This first phase leads to the controlled trafficking of receptors and signaling mediators involved in folding endosomes to the cellular interface, which allows the transfer of information from T cells to APCs through IS formation. Discrimination of self and nonself antigen is somatically learned in adaptive immunity. In an autoimmune condition such as AS, there is a disturbance of self/nonself antigen discrimination; available findings imply that the IS plays a preeminent role in the adaptive immune response. In this paper, we provide insights into the genesis of AS by evaluating recent developments in the function of vesicular trafficking in IS formation and the targeted release of exosomes enriched microRNAs (miRNA) at the synaptic region in T cells.