bluuebunny/crossref_metadata_2025 huggingface dataset:
NGOs and the Neo-Liberalization of Political Theatre in Pakistan: Ajoka’s Surrender to the Politics of Rights
Authors: Abbas Qaisar
Published: February 2024

The neoliberal enterprise of NGOs has transformed the left-leaning politics of the political theatre movement in the Punjab region of Pakistan. Commencing in the 1980s, this theatre acted as a vibrant movement of the Left, challenging the brutal military dictatorship of General Zia. At a later stage, its politics changed to the neoliberal politics of NGOs, giving way to economics and the agenda of international donor organizations of the Global North. This article demonstrates the turn-around of theatre company Ajoka’s recent production Saira aur Miara (2019) and focuses on the production’s politics, together with its text, design, and performance modes in aesthetic terms. A materialist and context-specific political approach examines to what extent class struggle and leftist ideas inform this company’s ideological imaginings and how much it has moved away from its original political position. It indicates the tensions and contradictions that have been created during this change and because of it.
Post Scriptum Budapest: The Tenth International Meeting of Theatre (MITEM)
Authors: Shevtsova Maria
Published: February 2024

MITEM (Mádach International Theatre Meeting) celebrated its tenth anniversary in October 2023, somewhat out of sync and out of time because of the festival’s cancellation due to Covid in 2020. It was absorbed into the Theatre Olympics (as discussed in New Theatre Quarterly, XXXIX, No. 4 (November 2023) [NTQ 156], p. 377–86), but its most recent edition was something of a replacement for the cancelled event, presented in the round figure of ten that has made everybody happy. It has also allowed this editor to follow through with a ‘PS’ to that article, acknowledging the importance of MITEM for both the National Theatre in Budapest and the theatregoing public. By contrast with the three and a half months of the Theatre Olympiad, MITEM lasted a modest twenty-four days.
From writing to record-keeping: a study of York’s civic administrative literacy, 1272–1307
Authors: Yi Jinming
Published: February 2024

Using York during the late thirteenth and early fourteenth centuries as a case-study, this article discusses a pivotal aspect of the development of civic administrative literacy: the inception of record-keeping. Previous historians have failed to note the evident advancement in York’s civic administrative literacy during the late thirteenth century, and they have usually dated the earliest surviving urban records to the mid-fourteenth century. By comparing different classes of civic and ecclesiastical records, this article reveals that York’s civic administration was in fact engaged in archival preservation from the late thirteenth century. In addition, by examining evidence that appears sporadically in royal archives, this article argues that the commencement of York’s urban archive was significantly influenced by the policies and archival activities of the royal government. Overall, this article aims to contribute to the literature on the early history of civic administrative literacy.
Psychological Distress and Domestic Violence Under COVID-19 Lockdown in LGBT+ Persons in Belgium, Germany, and Portugal
Authors: De Schrijver Lotte , Fomenko Elizaveta , Schuster Isabell , Tomaszewska Paulina , Dias Sonia , Keygnaert Ines
Published: February 2024

Abstract: Background: During the COVID-19 pandemic, governments worldwide issued lockdown and social-distancing measures, which triggers psychological distress and may increase the occurrence of domestic violence (DV). We examined the role of the LGBT+ status in the relationship between risk factors of DV and its occurrence during the pandemic. Methods: In this cross-sectional study (n = 5,148), an online self-report questionnaire was administered to a non-probabilistic sample of participants living in Belgium, Germany, and Portugal between March and June 2020. Participants were sampled through national media, social media, and snowballing procedures. Results: LGBT+ persons reported significantly higher levels of perceived stress, more acute stress symptoms, and more illegal drug use during the first weeks of the lockdown compared to non-LGBT+ persons. DV in LGBT+ persons was not more prevalent than in the non-LGBT under COVID-19 lockdown measures. No significant evidence was found indicating that identifying as LGBT+ itself increases the risk of DV exposure. Conclusion: LGBT+ persons were more at risk of DV compared to non-LGBT+ persons prior to but not during COVID-19 lockdown measures. The increased risk for DV can be linked to socio-demographic characteristics, levels of stress, and illegal drug use which are more prevalent in LGBT+ persons than in non-LGBT+ persons.
Long‐Term Stability, Noise, and Temperature Sensitivity of Modular Porous‐Pot Electrodes Designed for Geophysical and Geotechnical Applications, and Details of Their Construction
Authors: Comeau Matthew J. , Ueding Stefan , Becken Michael
Published: February 2024

Electrodes are used to measure a potential difference between two points. In geophysical and geotechnical applications they are often in the form of non‐polarizable porous‐pot electrodes. Here we describe the design, construction, and testing of modular and refillable electrodes, which facilitates repair as the electrodes degrade over time. We use a chemical composition based on a metal in contact with an over‐saturated electrolyte that consists of a salt of that metal and an auxiliary salt. We compare characteristics when the electrolyte is stabilized in a clay or not, and with various states of ceramic porous plugs and two types of wood plugs. Next, we assess the long‐term stability (more than 1 month), noise (periods of 1 s to 1 hr), and temperature sensitivity of different types of electrodes. Electrodes with an electrolyte and clay formula showed lower noise (0.2–0.4 μV at periods of 1–120 s), greater long‐term stability (0.05–0.5 mV/month of smooth drift), and greater consistency between samples measured than those with no clay (noise and drift values up to four times larger). The effects from different porous plugs were negligible, with similar results for ceramic and wood types. The temperature sensitivity of the electric potential was assessed, from −3 to 35°C. All electrodes showed a temperature sensitivity of about −30 μV/°C. This is considered very low compared to some commercially available electrodes. Finally, continuous long‐term laboratory and field measurements of the potential highlight the application of the new electrodes.
Incorporating Intensity Distance Attenuation Into PLUM Ground‐Motion‐Based Earthquake Early Warning in the United States: The APPLES Configuration
Authors: Saunders Jessie K. , Cochran Elizabeth S. , Bunn Julian J. , Baltay Annemarie S. , Minson Sarah E. , O’Rourke Colin T.
Published: February 2024

We develop Attenuated ProPagation of Local Earthquake Shaking (APPLES), a new configuration for the United States West Coast version of the Propagation of Local Undamped Motion (PLUM) earthquake early warning (EEW) algorithm that incorporates attenuation into its ground‐motion prediction procedures. Under APPLES, instead of using a fixed radius to forward‐predict observed peak ground shaking to the area surrounding a seismic station, the forward‐predicted intensity at a location depends on the distance from the station using an intensity prediction relationship. We conduct conceptual tests of maximum intensity distribution predictions in APPLES and PLUM using a catalog of ShakeMaps to confirm that the attenuation relationship in APPLES is appropriately modeling shaking distributions for West Coast earthquakes. Then, we run APPLES and PLUM in simulated real‐time tests to determine warning time performance. Finally, we compare real‐time alert behavior during the 2022 M6.4 Ferndale, California, earthquake and other recent events. We find that APPLES presents two potential improvements to PLUM by reducing over‐alerting during smaller magnitude earthquakes and by increasing warning times in some locations during larger earthquakes. APPLES can produce missed and late alerts in locations that experience shaking intensities close to the level used to issue alerts, so preferred alerting strategies with APPLES would use alert thresholds that are lower than the intensities targeted for EEW alerts. We find alerts using APPLES are also similar to those for the source‐based approaches currently used in the ShakeAlert EEW system, which will make APPLES easier to integrate into the system.
A Novel Approach to Impact Crater Mapping and Analysis on Enceladus, Using Machine Learning
Authors: Blanco‐Rojas M. , Carroll M. L. , Spradlin C. S. , Caraballo‐Vega J. A. , Williams Z. W.
Published: February 2024

Impact cratering is one of the most important processes shaping planetary surfaces, offering valuable clues about the target body's geologic history and composition. However, crater mapping has historically been done manually, a process that has proven to be both arduous and time consuming. This paper outlines a machine learning crater mapping approach for bodies with limited elevation data available (Digital Elevation Models). We applied a Convolutional Neural Network for the detection and morphometry of impact craters on Saturn's moon Enceladus using light‐shadow labels trained on data from the Cassini Imaging Science Subsystem. Our algorithm identified a total of 5,240 features which were used to quantify crater distribution; this included the highest number of small craters (<1–2 km in diameter) recorded on Enceladus by any previous published study. The pool of features was later down‐selected to craters between 0 and 30°N (latitude) imaged at high incidence (>60°) and phase angles (>26°). The down selection was necessary to accurately perform diameter measurements and derive depths from shadow estimation techniques to calculate depth–diameter ratios (d/D); a well‐studied relationship used to constrain planetary surface properties. Results show that the d/D ratio of craters in the equatorial region of Enceladus range from ∼0.06 to 0.37, with a median of 0.19. Our results will inform efforts to constrain the surface properties of this region of Enceladus, potentially also supporting future mission concept design for the Saturnian moon. Future work will explore the simple‐to‐complex crater transition and differences between this area's d/D and Enceladus' northern and southern latitudes.
Bipolar patients display stoichiometric imbalance of gene expression in post-mortem brain samples
Authors: Holmgren Asbjørn , Akkouh Ibrahim , O’Connell Kevin Sean , Osete Jordi Requena , Bjørnstad Pål Marius , Djurovic Srdjan , Hughes Timothy
Published: February 2024

Bipolar disorder is a severe neuro-psychiatric condition where genome-wide association and sequencing studies have pointed to dysregulated gene expression as likely to be causal. We observed strong correlation in expression between GWAS-associated genes and hypothesised that healthy function depends on balance in the relative expression levels of the associated genes and that patients display stoichiometric imbalance. We developed a method for quantifying stoichiometric imbalance and used this to predict each sample’s diagnosis probability in four cortical brain RNAseq datasets. The percentage of phenotypic variance on the liability-scale explained by these probabilities ranged from 10.0 to 17.4% (AUC: 69.4–76.4%) which is a multiple of the classification performance achieved using absolute expression levels or GWAS-based polygenic risk scores. Most patients display stoichiometric imbalance in three to ten genes, suggesting that dysregulation of only a small fraction of associated genes can trigger the disorder, with the identity of these genes varying between individuals.
Autophagy-related gene model as a novel risk factor for schizophrenia
Authors: Tan Yunfei , Zhu Junpeng , Hashimoto Kenji
Published: February 2024

Autophagy, a cellular process where cells degrade and recycle their own components, has garnered attention for its potential role in psychiatric disorders, including schizophrenia (SCZ). This study aimed to construct and validate a new autophagy-related gene (ARG) risk model for SCZ. First, we analyzed differential expressions in the GSE38484 training set, identifying 4,754 differentially expressed genes (DEGs) between SCZ and control groups. Using the Human Autophagy Database (HADb) database, we cataloged 232 ARGs and pinpointed 80 autophagy-related DEGs (AR-DEGs) after intersecting them with DEGs. Subsequent analyses, including metascape gene annotation, pathway and process enrichment, and protein-protein interaction enrichment, were performed on the 80 AR-DEGs to delve deeper into their biological roles and associated molecular pathways. From this, we identified 34 candidate risk AR-DEGs (RAR-DEGs) and honed this list to final RAR-DEGs via a constructed and optimized logistic regression model. These genes include VAMP7, PTEN, WIPI2, PARP1, DNAJB9, SH3GLB1, ATF4, EIF4G1, EGFR, CDKN1A, CFLAR, FAS, BCL2L1 and BNIP3. Using these findings, we crafted a nomogram to predict SCZ risk for individual samples. In summary, our study offers deeper insights into SCZ’s molecular pathogenesis and paves the way for innovative approaches in risk prediction, gene-targeted diagnosis, and community-based SCZ treatments.
The golden key to open mystery boxes of SMARCA4-deficient undifferentiated thoracic tumor: focusing immunotherapy, tumor microenvironment and epigenetic regulation
Authors: Li Xiang , Tian Sen , Shi Hui , Ta Na , Ni Xiang , Bai Chenguang , Zhu Zhanli , Chen Yilin , Shi Dongchen , Huang Haidong , Chen Longpei , Hu Zhenhong , Qu Lei , Fang Yao , Bai Chong
Published: February 2024

SMARCA4-deficient undifferentiated thoracic tumor is extremely invasive. This tumor with poor prognosis is easily confused with SMARCA4-deficent non-small cell lung cancer or sarcoma. Standard and efficient treatment has not been established. In this review, we summarized the etiology, pathogenesis and diagnosis, reviewed current and proposed innovative strategies for treatment and improving prognosis. Immunotherapy, targeting tumor microenvironment and epigenetic regulator have improved the prognosis of cancer patients. We summarized clinicopathological features and immunotherapy strategies and analyzed the progression-free survival (PFS) and overall survival (OS) of patients with SMARCA4-UT who received immune checkpoint inhibitors (ICIs). In addition, we proposed the feasibility of epigenetic regulation in the treatment of SMARCA4-UT. To our knowledge, this is the first review that aims to explore innovative strategies for targeting tumor microenvironment and epigenetic regulation and identify potential benefit population for immunotherapy to improve the prognosis.
Pathway level subtyping identifies a slow-cycling biological phenotype associated with poor clinical outcomes in colorectal cancer
Authors: Malla Sudhir B. , Byrne Ryan M. , Lafarge Maxime W. , Corry Shania M. , Fisher Natalie C. , Tsantoulis Petros K. , Mills Megan L. , Ridgway Rachel A. , Lannagan Tamsin R. M. , Najumudeen Arafath K. , Gilroy Kathryn L. , Amirkhah Raheleh , Maguire Sarah L. , Mulholland Eoghan J. , Belnoue-Davis Hayley L. , Grassi Elena , Viviani Marco , Rogan Emily , Redmond Keara L. , Sakhnevych Svetlana , McCooey Aoife J. , Bull Courtney , Hoey Emily , Sinevici Nicoleta , Hall Holly , Ahmaderaghi Baharak , Domingo Enric , Blake Andrew , Richman Susan D. , Isella Claudio , Miller Crispin , Bertotti Andrea , Trusolino Livio , Loughrey Maurice B. , Kerr Emma M. , Tejpar Sabine ,   S:CORT consortium, Maughan Timothy S. , Lawler Mark , Campbell Andrew D. , Leedham Simon J. , Koelzer Viktor H. , Sansom Owen J. , Dunne Philip D.
Published: February 2024

Molecular stratification using gene-level transcriptional data has identified subtypes with distinctive genotypic and phenotypic traits, as exemplified by the consensus molecular subtypes (CMS) in colorectal cancer (CRC). Here, rather than gene-level data, we make use of gene ontology and biological activation state information for initial molecular class discovery. In doing so, we defined three pathway-derived subtypes (PDS) in CRC: PDS1 tumors, which are canonical/LGR5+ stem-rich, highly proliferative and display good prognosis; PDS2 tumors, which are regenerative/ANXA1+ stem-rich, with elevated stromal and immune tumor microenvironmental lineages; and PDS3 tumors, which represent a previously overlooked slow-cycling subset of tumors within CMS2 with reduced stem populations and increased differentiated lineages, particularly enterocytes and enteroendocrine cells, yet display the worst prognosis in locally advanced disease. These PDS3 phenotypic traits are evident across numerous bulk and single-cell datasets, and demark a series of subtle biological states that are currently under-represented in pre-clinical models and are not identified using existing subtyping classifiers.
A high-quality genome assembly of the waterlily aphid Rhopalosiphum nymphaeae
Authors: Wang Yangzi , Xu Shuqing
Published: February 2024

Waterlily aphid, Rhopalosiphum nymphaeae (Linnaeus), is a host-alternating aphid known to feed on both terrestrial and aquatic hosts. It causes damage through direct herbivory and acting as a vector for plant viruses, impacting worldwide Prunus spp. fruits and aquatic plants. Interestingly, R. nymphaeae’s ability to thrive in both aquatic and terrestrial conditions sets it apart from other aphids, offering a unique perspective on adaptation. We present the first high-quality R. nymphaeae genome assembly with a size of 324.4 Mb using PacBio long-read sequencing. The resulting assembly is highly contiguous with a contig N50 reached 12.7 Mb. The BUSCO evaluation suggested a 97.5% completeness. The R. nymphaeae genome consists of 16.9% repetitive elements and 16,834 predicted protein-coding genes. Phylogenetic analysis positioned R. nymphaeae within the Aphidini tribe, showing close relations to R. maidis and R. padi. The high-quality reference genome R. nymphaeae provides a unique resource for understanding genome evolution in aphids and paves the foundation for understanding host plant adaptation mechanisms and developing pest control strategies.
Street trees provide an opportunity to mitigate urban heat and reduce risk of high heat exposure
Authors: Ettinger Ailene K. , Bratman Gregory N. , Carey Michael , Hebert Ryan , Hill Olivia , Kett Hannah , Levin Phillip , Murphy-Williams Maia , Wyse Lowell
Published: February 2024

Climate change is exacerbating the need for urban greening and the associated environmental and human well-being benefits. Trees can help mitigate urban heat, but more detailed understanding of cooling effects of green infrastructure are needed to guide management decisions and deploy trees as effective and equitable climate adaptation infrastructure. We investigated how urban trees affect summer air temperature along sidewalks within a neighborhood of Tacoma, Washington, USA, and to what extent urban trees reduce risks of high summer temperatures (i.e., the levels regulated by state outdoor heat exposure rules intended to reduce heat-related illnesses). Air temperature varied by 2.57 °C, on average, across our study area, and the probability of daytime temperatures exceeding regulated high temperature thresholds was up to five times greater in locations with no canopy cover within 10 m compared to those with 100% cover. Air temperatures decreased linearly with increasing cover within 10 m, suggesting that every unit of added tree cover can help cool the air. Our findings highlight the value of trees in mitigating urban heat, especially given expected warming with climate change. Protecting existing urban trees and increasing tree cover (e.g., by planting street trees), are important actions to enhance climate change resilience of urban areas.
Pharmacological HIF-1 activation upregulates extracellular vesicle production synergistically with adiponectin through transcriptional induction and protein stabilization of T-cadherin
Authors: Fujii Kohei , Fujishima Yuya , Kita Shunbun , Kawada Keitaro , Fukuoka Keita , Sakaue Taka-aki , Okita Tomonori , Kawada-Horitani Emi , Nagao Hirofumi , Fukuda Shiro , Maeda Norikazu , Nishizawa Hitoshi , Shimomura Iichiro
Published: February 2024

Pharmacological activation of hypoxia-inducible factor 1 (HIF-1), a hypoxia-responsive transcription factor, has attracted increasing attention due to its efficacy not only in renal anemia but also in various disease models. Our study demonstrated that a HIF-1 activator enhanced extracellular vesicle (EV) production from cultured endothelial cells synergistically with adiponectin, an adipocyte-derived factor, through both transcriptional induction and posttranscriptional stabilization of an adiponectin binding partner, T-cadherin. Increased EV levels were observed in wild-type mice but not in T-cadherin null mice after consecutive administration of roxadustat. Adiponectin- and T-cadherin-dependent increased EV production may be involved in the pleiotropic effects of HIF-1 activators.
Land cover changes across Greenland dominated by a doubling of vegetation in three decades
Authors: Grimes Michael , Carrivick Jonathan L. , Smith Mark W. , Comber Alexis J.
Published: February 2024

Land cover responses to climate change must be quantified for understanding Arctic climate, managing Arctic water resources, maintaining the health and livelihoods of Arctic societies and for sustainable economic development. This need is especially pressing in Greenland, where climate changes are amongst the most pronounced of anywhere in the Arctic. Ice loss from the Greenland Ice Sheet and from glaciers and ice caps has increased since the 1980s and consequently the proglacial parts of Greenland have expanded rapidly. Here we determine proglacial land cover changes at 30 m spatial resolution across Greenland during the last three decades. Besides the vastly decreased ice cover (− 28,707 km2 ± 9767 km2), we find a doubling in total areal coverage of vegetation (111% ± 13%), a quadrupling in wetlands coverage (380% ± 29%), increased meltwater (15% ± 15%), decreased bare bedrock (− 16% ± 4%) and increased coverage of fine unconsolidated sediment (4% ± 13%). We identify that land cover change is strongly associated with the difference in the number of positive degree days, especially above 6 °C between the 1980s and the present day. Contrastingly, absolute temperature increase has a negligible association with land cover change. We explain that these land cover changes represent local rapid and intense geomorphological activity that has profound consequences for land surface albedo, greenhouse gas emissions, landscape stability and sediment delivery, and biogeochemical processes.
Enhancing automotive cooling systems: composite fins and nanoparticles analysis in radiators
Authors: Ramesh Kumar R. , Karthik K. , Elumalai P. V. , Elumalai R. , Chandran Davannendran , Prakash E. , Hassin Nasim
Published: February 2024

Composites are driving positive developments in the automobile sector. In this study investigated the use of composite fins in radiators using computational fluid dynamics (CFD) to analyze the fluid-flow phenomenon of nanoparticles and hydrogen gas. Our world is rapidly transforming, and new technologies are leading to positive revolutions in today’s society. In this study successfully analyzed the entire thermal simulation processes of the radiator, as well as the composite fin arrangements with stress efficiency rates. The study examined the velocity path, pressure variations, and temperature distribution in the radiator setup. As found that nanoparticles and composite fins provide superior thermal heat rates and results. The combination of an aluminum radiator and composite fins in future models will support the control of cooling systems in automotive applications. The final investigation statement showed a 12% improvement with nanoparticles, where the velocity was 1.61 m/s and the radiator system’s pressure volume was 2.44 MPa. In the fin condition, the stress rate was 3.60 N/mm2.
Prospective health care costs and lost work days associated with diabetes-related distress and depression symptoms among 1488 individuals with diabetes
Authors: Sommer Jana , Linnenkamp Ute , Gontscharuk Veronika , Andrich Silke , Brüne Manuela , Schmitz-Losem Imke , Kruse Johannes , Evers Silvia M. A. A. , Hiligsmann Mickaël , Hoffmann Barbara , Icks Andrea
Published: February 2024

The aim of this study was to investigating the impact of major depression symptoms and diabetes-related distress on future health care costs and lost workdays in individuals with diabetes. We linked survey data from a random sample of a German statutory health insurance (SHI) with diabetes (n = 1488, 63.0% male, mean age 66.9 years) with their SHI data one year after the survey. Within the survey data we identified major depression symptoms (Patient Health Questionnaire-9) and diabetes-related distress (Problem Areas in Diabetes Scale). We retrieved health care costs and lost workdays from SHI data. To assess the impact of major depression symptoms and diabetes-related distress on health care costs and lost workdays, we adjusted regression models for age, sex, education, employment status, and diabetes duration, type, and severity. Major depression symptoms were associated with significantly higher costs (by a factor of 1.49; 95% CI: 1.18–1.88). Lost workdays were also more likely for respondents with depression symptoms (RR1.34; 0.97–1.86). Health care costs (by a factor of 0.81; 0.66–1.01) and the risk of lost workdays (RR 0.86; 0.62–1.18) may be lower among respondents with high diabetes-related distress. While major depression and diabetes-related distress have overlapping indicators, our results indicate different impacts on health care costs.
Transcriptome analysis of Sparidentex hasta larvae exposed to water-accommodated fraction of Kuwait crude oil
Authors: Kumar Vinod , Karam Qusaie , Shajan Anisha B. , Al-Nuaimi Sabeeka , Sattari Zainab , El-Dakour Saleem
Published: February 2024

Anthropogenic activities have been shown to significantly affect marine life. Water pollution and oil spills are particularly deleterious to the fish population, especially during their larval stage. In this study, Sobaity-sea bream Sparidentex hasta (Valenciennes, 1830) larvae were exposed to serial dilutions of water-accommodated fraction of Kuwait crude oil (KCO-WAF) for varying durations (3, 6, 24, 48, 72 or 96 h) in acute exposure regime. Gene expression was assessed using RNA sequencing and validated through RT-qPCR. The RNA sequencing data were aligned to the sequenced genome, and differentially expressed genes were identified in response to treatment with or without KCO-WAF at various exposure times. The highest number of differentially expressed genes was observed at the early time point of 6 h of post-exposure to KCO-WAF. The lowest number of differentially expressed genes were noticed at 96 h of treatment indicating early response of the larvae to KCO-WAF contaminant. The acquired information on the differentially expressed genes was then used for functional and pathway analysis. More than 90% of the differentially expressed genes had a significant BLAST match, with the two most common matching species being Acanthopagrus latus and Sparus aurata. Approximately 65% of the differentially expressed genes had Gene Ontology annotations, whereas > 35% of the genes had KEGG pathway annotations. The differentially expressed genes were found to be enriched for various signaling pathways (e.g., MAPK, cAMP, PI3K-Akt) and nervous system-related pathways (e.g., neurodegeneration, axon guidance, glutamatergic synapse, GABAergic synapse). Early exposure modulated the signaling pathways, while KCO-WAF exposure of larvae for a longer duration affected the neurodegenerative/nervous system-related pathways. RT-qPCR analysis confirmed the differential expression of genes at each time point. These findings provide insights into the underlying molecular mechanisms of the deleterious effects of acute exposure to oil pollution—on marine fish populations, particularly at the early larval stage of Sparidentex hasta.
A new small duckbilled dinosaur (Hadrosauridae: Lambeosaurinae) from Morocco and dinosaur diversity in the late Maastrichtian of North Africa
Authors: Longrich Nicholas R. , Pereda-Suberbiola Xabier , Bardet Nathalie , Jalil Nour-Eddine
Published: February 2024

In the Late Cretaceous, northern and southern hemispheres evolved distinct dinosaurian faunas. Titanosaurians and abelisaurids dominated the Gondwanan continents; hadrosaurids, ceratopsians and tyrannosaurs dominated North America and Asia. Recently, a lambeosaurine hadrosaurid,Ajnabia odysseus, was reported from the late Maastrichtian phosphates of the Oulad Abdoun Basin Morocco, suggesting dispersal between Laurasia and Gondwana. Here we report new fossils from the phosphates of Morocco showing lambeosaurines achieved high diversity in the late Maastrichtian of North Africa. A skull represents a new dwarf lambeosaurine,Minqaria bata.MinqariaresemblesAjnabia odysseusin size, but differs in the ventrally positioned jugal facet and sinusoidal toothrow. The animal is small, ~ 3.5 m long, but the fused braincase shows it was mature. A humerus and a femur belong to larger hadrosaurids, ~ 6 m long, implying at least three species coexisted. The diversity of hadrosaurids in Europe and Africa suggests a dispersal-driven radiation, with lambeosaurines diversifying to take advantage of low ornithischian diversity. African lambeosaurines are small compared to North American and Asia hadrosaurids however, perhaps due to competition with titanosaurians. Hadrosaurids are unknown from eastern Africa, suggesting Moroccan hadrosaurids may be part of a distinct insular fauna, and represent an island radiation.
A deepfake-based study on facial expressiveness and social outcomes
Authors: Renier Laetitia A. , Shubham Kumar , Vijay Rahil Satyanarayan , Mishra Swasti Shreya , Kleinlogel Emmanuelle P. , Jayagopi Dinesh Babu , Schmid Mast Marianne
Published: February 2024

Artificial intelligence (AI)-generated media is used in entertainment, art, education, and marketing. AI-generated faces or facial expressions using deepfake-based technologies might also contribute to nonverbal behavior studies. As a proof of concept, in this research, we test the replicability of past results regarding the positive effects of facial expressiveness (e.g., gazing, nodding, and smiling) on social outcomes. Because the facial expressions when listening to someone (i.e., nonverbal immediacy) encompass several behaviors that are typically related (i.e., smiling while nodding), the effect of combinations of these behaviors is challenging to study. We thus test the effect of facial expressiveness (gazing, nodding, and smiling vs. none) on first impression judgements in a job interview setting using AI-generated experimental video material. We measure how competent, warm, and favorably independent observers (n = 823) perceive the targets (n = 159) showing AI-generated facial expressions. Our results replicate past research showing that the more targets are facially expressive, the more they are perceived favorably. Apart from supporting evidence of the importance of facial expressiveness for conveying a positive impression, we show the benefits of relying on AI-generated experimental video material for the study of nonverbal behavior.
Association between arterial stiffness and autonomic dysfunction in participants underwent treadmill exercise testing: a cross-sectional analysis
Authors: Park Sungjoon , Kim Hack-Lyoung , Park Kyung-Taek , Joh Hyun Sung , Lim Woo-Hyun , Seo Jae-Bin , Kim Sang-Hyun , Kim Myung-A
Published: February 2024

Data on the impact of arterial stiffness on autonomic function are limited. We sought to investigate whether heart rate recovery (HRR), a predictor of autonomic function, is impaired in patients with increased arterial stiffness. A total of 475 participants (mean age 55.8 ± 11.1 years, 34.3% women) who underwent a treadmill exercise test (TET) for the evaluation of chest pain were retrospectively analyzed. All patients underwent brachial-ankle pulse wave velocity (baPWV) measurement on the same day. HRR was defined as the difference in heart rate from maximal exercise to 1 min of recovery. Participants with the lowest HRR tertile were older and had more cardiovascular risk factors than those with the highest HRR tertile. Simple correlation analysis showed that baPWV was negatively correlated with HRR (r =  − 0.327, P < 0.001). In multiple linear regression analysis, there was a significant association between baPWV and HRR, even after adjusting for potential confounders (β =  − 0.181, P < 0.001). In participants who underwent TET, baPWV was negatively correlated with HRR. The results of our study indicate a potential relationship between arterial stiffness and the autonomic nervous system.
Trajectories of resting energy expenditure and performance of predictive equations in children hospitalized with an acute illness and malnutrition: a longitudinal study
Authors: Afroze Farzana , Khoshnevisan Farnaz , Harawa Philliness Prisca , Islam Zahidul , Bourdon Celine , Khoswe Stanley , Islam Munirul , Sarker Shafiqul Alam , Islam Farhana , Sayeem Bin Shahid Abu Sadat Mohammad , Joosten Koen , Hulst Jessie M. , Eneya Chisomo , Walson Judd L. , Berkley James A. , Potani Isabel , Voskuijl Wieger , Ahmed Tahmeed , Chisti Mohammod Jobayer , Bandsma Robert H. J.
Published: February 2024

There is scarce data on energy expenditure in ill children with different degrees of malnutrition. This study aimed to determine resting energy expenditure (REE) trajectories in hospitalized malnourished children during and after hospitalization. We followed a cohort of children in Bangladesh and Malawi (2–23 months) with: no wasting (NW); moderate wasting (MW), severe wasting (SW), or edematous malnutrition (EM). REE was measured by indirect calorimetry at admission, discharge, 14-and-45-days post-discharge. 125 children (NW, n = 23; MW, n = 29; SW, n = 51; EM, n = 22), median age 9 (IQR 6, 14) months, provided 401 REE measurements. At admission, the REE of children with NW and MW was 67 (95% CI [58, 75]) and 70 (95% CI [63, 76]) kcal/kg/day, respectively, while REE in children with SW was higher, 79 kcal/kg/day (95% CI [74, 84], p = 0.018), than NW. REE in these groups was stable over time. In children with EM, REE increased from admission to discharge (65 kcal/kg/day, 95% CI [56, 73]) to 79 (95% CI [72, 86], p = 0.0014) and was stable hereafter. Predictive equations underestimated REE in 92% of participants at all time points. Recommended feeding targets during the acute phase of illness in severely malnourished children exceeded REE. Acutely ill malnourished children are at risk of being overfed when implementing current international guidelines.
An analytical performance approach for RCS/RS with one robot serving multiple stack heights under a one-path relocation strategy
Authors: Trost Philipp , Eder Michael
Published: February 2024

Robotic compact storage and retrieval systems (RCS/RS) represent a modern and useful storage system since the number of installed systems is growing fast. The modularity and demand-based scalability are reasons, therefore. Nonetheless, there are hardly any statements on the performance of those warehouses. This paper presents an analytical calculation approach to determine the performance of an RCS/RS with one operating robot serving different grid sizes and a varying number of stacked containers. The robot’s cycle time is calculated by assuming a uniform distribution of container stacks and a probabilistic storage height. A discrete-event simulation model of an RCS/RS is built to verify and validate the analytical approximations. The system’s basic structure and the input parameters originate from a European material handling provider. After the verification and validation, an extensive parameter variation is done with the target of displaying a wide range of usage. This analytical approach, which is easy and fast solvable with standard calculation programs, represents an easy and fast tool to predict the performance of one robot operating in an RCS/RS for any system configuration.
Assessing morphological preservation of gastrointestinal parasites from fecal samples of wild capuchin monkeys (Cebus imitator) stored in ethanol versus formalin
Authors: Hass Joelle K. , Henriquez Megan C. , Churcher Jessica , Hamou Hadjira , Morales Suheidy Romero , Melin Amanda D.
Published: February 2024

The copromicroscopic identification of gastrointestinal parasites is a common, cost-effective method vital to understanding host-parasite interactions. However, its efficacy depends on effective preservation of the samples. In this study, we compare the preservation of ethanol and formalin preserved gastrointestinal parasites collected from a wild population of Costa Rican capuchin monkeys (Cebus imitator). Fecal samples were collected, halved, and stored in either 10% formalin or 96% ethanol at ambient temperature, then microscopically screened for the presence of parasites. Parasites were morphologically identified and rated based on their preservation using a newly developed rubric. We identified more parasitic morphotypes in formalin-preserved samples but found no difference in the number of parasites per fecal gram (PFG) between mediums. There was no difference in the PFG of two most prevalent parasite morphotypes, Filariopsis barretoi larvae and Strongyle-type eggs, and while Filariopsis larvae were better preserved in formalin, strongyle eggs showed no preservation difference between mediums. Our results support the suitability of both ethanol and formalin for morphological parasite identification in samples stored over 1 year, describe the morphological changes and challenges associated with parasite degradation, and highlight the potential for future studies to use both morphological and molecular methods in non-invasively collected samples.
Unveiling origins, composition, and appearance of ancient Islamic gold coins through elemental and smartphone-based colorimetric studies
Authors: Sáez-Hernández Roberto , Luque María Josefa , Mauri-Aucejo Adela R. , Morales-Rubio Ángel , Cervera M. Luisa
Published: February 2024

In this article, the Islamic gold coins collections of the University of Valencia is studied for the first time for its elemental composition and colorimetric properties. To that end, non-destructive elemental analysis using energy-dispersive X-ray fluorescence is applied to obtain the coins’ elemental profile. Additionally, the colour of the coins is assessed using smartphone-based colorimetry as an innovative non-invasive method. Results indicate that the Islamic coins could be attributed to Almoravids, and the gold origin could be the famous Sudanese gold, an ore which was valued all over the world. Also, the text found in the coins was translated and allowed to objectively identify the mint and year. Based on these results, it can be seen that the earliest coins struck in the Iberian Peninsula are characterised by slightly lower gold concentrations than the ones struck in the northern part of Africa, pointing towards a potential recycling of coins which already circulated in the area. In conclusion, this work provides new analytical insights into a peculiar and unique type of samples, allowing to draw some conclusions in terms of their origins and materials, and for the first time allows to characterise the chromatic coordinates of this type of samples.
Empirical dynamic modelling and enhanced causal analysis of short-length Culex abundance timeseries with vector correlation metrics
Authors: Kollas Nikos , Gewehr Sandra , Kioutsioukis Ioannis
Published: February 2024

Employing Empirical Dynamic Modelling we investigate whether model free methods could be applied in the study of Culex mosquitoes in Northern Greece. Applying Simplex Projection and S-Map algorithms on yearly timeseries of maximum abundances from 2011 to 2020 we successfully predict the decreasing trend in the maximum number of mosquitoes which was observed in the rural area of Thessaloniki during 2021. Leveraging the use of vector correlation metrics we were able to deduce the main environmental factors driving mosquito abundance such as temperature, rain and wind during 2012 and study the causal interaction between neighbouring populations in the industrial area of Thessaloniki between 2019 and 2020. In all three cases a chaotic and non-linear behaviour of the underlying system was observed. Given the health risk associated with the presence of mosquitoes as vectors of viral diseases these results hint to the usefulness of EDM methods in entomological studies as guides for the construction of more accurate and realistic mechanistic models which are indispensable to public health authorities for the design of targeted control strategies and health prevention measures.
Highly pathogenic avian influenza virus H5N1 clade 2.3.4.4b from Peru forms a monophyletic group with Chilean isolates in South America
Authors: Castro-Sanguinetti Gina R. , González-Veliz Rosa , Callupe-Leyva Alonso , Apaza-Chiara Ana P. , Jara Javier , Silva Walter , Icochea Eliana , More-Bayona Juan A.
Published: February 2024

Highly pathogenic avian Influenza virus (HPAIV) has spread in an unprecedented extent globally in recent years. Despite the large reports of cases in Asia, Europe, and North America, little is known about its circulation in South America. Here, we describe the isolation, and whole genome characterization of HPAIV obtained from sampling 26 wild bird species in Peru, representing one of the largest studies in our region following the latest HPAIV introduction in South America. Out of 147 samples analyzed, 22 were positive for detection of avian influenza virus using a qRT-PCR-based assay. Following inoculation into embryonated chicken eggs, fourteen viral isolates were obtained from which nine isolates were selected for genome characterization, based on their host relevance. Our results identified the presence of HPAIV H5N1 subtype in a highly diverse wild bird species. Phylogenetic analysis revealed that these isolates correspond to the clade 2.3.4.4b, sharing a common ancestor with North American isolates and forming a monophyletic group along with isolates from Chile. Altogether, changes at the amino acid levels compared to their closest relatives indicates the virus is evolving locally, highlighting the need for constant genomic surveillance. This data evidence the chances for spillover events increases as the virus spreads into large populations of immunologically naïve avian species and adding conditions for cross species transmission.
Effects of weights applied to the apex of a bag-valve-mask and pinch strength on tidal volume: a prospective simulation study
Authors: Uhm Dong Choon , Kim A Jung , Koh Bong Yeun , Lee Kui Ja
Published: February 2024

A bag-valve-mask (BVM) is a first aid tool that can easily and quickly provide positive-pressure ventilation in patients with breathing difficulties. The most important aspect of BVM bagging is how closely the mask adheres to the patient’s face when the E–C technique is used. In particular, the greater the adhesion force at the apex of the mask, the greater the tidal volume. The purpose of this study was to investigate the effect of various weights applied to the mask’s apex and the pinch strength needed to perform the E–C technique, on tidal volume. In this prospective simulation study, quasi-experimental and equivalent time-series designs were used. A total of 72 undergraduate paramedic student from three universities were recruited using convenience sampling. The tidal volumes according to the weights (0 g, 100 g, 200 g, 300 g) applied to the apical area of the mask, handgrip strength, and pinch strength (tip pinch strength, key pinch strength, and tripod pinch strength) were measured. A linear mixed model analysis was performed. Linear mixed model analyses showed that tidal volume was significantly higher at 200 g (B = 43.38, p = 0.022) and 300 g (B = 38.74, p = 0.017) than at 0 g. Tripod pinch strength (B = 12.88, p = 0.007) had a significant effect on mask adhesion for effective BVM ventilation. Adding weight to the apical area of the mask can help maintain the E–C technique and enable effective ventilation. Future studies are required to develop specific strategies to improve the ventilation skills, which can be an important first-aid activity.
Breeding behavior analysis in a large captive colony of African penguins (Spheniscus demersus) and its implications for population management and conservation
Authors: Borecki Paweł , Rosenberger Joanna , Mucha Anna , Partyka Agnieszka
Published: February 2024

The African penguin Spheniscus demersus, frequently housed in zoos, holds potential for future reintroduction efforts due to its declining wild population. This paper aims to explore various aspects of reproductive performance in African penguins within a large ex situ colony at Zoo Wrocław in Poland, covering 9 years of breeding behaviors. The analysis reveals parallels in colony growth and partner change patterns with those observed in the wild. Positive correlations were found between breeding success and pair-bond duration, with the increasing colony size influencing reproductive performance. Contrary to their wild counterparts, captive African penguins initiate breeding attempt and produce a fertilized egg at a younger age. However, successful breeding still requires gaining experience or forming pairs with more experienced partners. Our research indicates that providing captive African penguins with unlimited food resources and sufficient nesting space results in rapid colony growth. The increased colony size facilitates breeding behaviors that positively influence population dynamics, particularly through the maintenance of long-term pair bond relationships and the potential for partner changes when necessary or desirable to enhance breeding success. We present compelling case studies in pair fidelity, offering valuable insights and implications for the management of captive populations and conservation efforts.
A virtual simulation approach to assess the effect of trocar-site placement and scar characteristics on the abdominal wall biomechanics
Authors: Tuset Lluís , López-Cano Manuel , Fortuny Gerard , López Josep M. , Herrero Joan , Puigjaner Dolors
Published: February 2024

Analyses of registries and medical imaging suggest that laparoscopic surgery may be penalized with a high incidence of trocar-site hernias (TSH). In addition to trocar diameter, the location of the surgical wound (SW) may affect TSH incidence. The intra-abdominal pressure (IAP) exerted on the abdominal wall (AW) might also influence the appearance of TSH. In the present study, we used finite element (FE) simulations to predict the influence of trocar location and SW characteristics (stiffness) on the mechanical behavior of the AW subject to an IAP. Two models of laparoscopy patterns on the AW, with trocars in the 5–12 mm range, were generated. FE simulations for IAP values within the 4 kPa–20 kPa range were carried out using the Code Aster open-source software. Different stiffness levels of the SW tissue were considered. We found that midline-located surgical wounds barely deformed, even though they moved outwards along with the regular LA tissue. Laterally located SWs hardly changed their location but they experienced significant variations in their volume and shape. The amount of deformation of lateral SWs was found to strongly depend on their stiffness. Trocar incisions placed in a LA with non-diastatic dimensions do not compromise its mechanical integrity. The more lateral the trocars are placed, the greater is their deformation, regardless of their size. Thus, to prevent TSH it might be advisable to close lateral trocars with a suture, or even use a prosthetic reinforcement depending on the patient's risk factors (e.g., obesity).