bluuebunny/crossref_metadata_2025 huggingface dataset:
Phytogenic Antioxidants Prolong n-3 Fatty Acid-Enriched Eggs’ Shelf Life by Activating the Nrf-2 Pathway through Phosphorylation of MAPK
Authors: Shahid Muhammad Suhaib , Zhou Shengyu , Nie Wei , Wang Liang , Lv Huiyuan , Yuan Jianmin
Published: October 2022

Helpful for human health, omega-3 (n-3)-enriched eggs are preferred by consumers. However, antioxidants should be added to the hen’s diet to prevent n-3 fatty acid oxidation due to their unsaturated bonds. A study was designed to investigate the effects of different antioxidants on performance, egg quality, fatty acid profile, oxidation parameters, gene expression, and magnum morphology. A total of 450 hens were divided into five dietary groups. Wheat–flaxseed was used for the basic diet (control) and supplemented with vitamin E (VE), chlorogenic acid (CA), polyphenol (PF), and lutein (L). The experiment lasted for 10 weeks. The eggs were collected on the 5th week and were analyzed for quality, oxidative stability, and fatty acid (FA) content, being stored for 0 d, 7 d, 14 d, 21 d, 28 d, 35 d, and 42 d. The results showed that supplemental VE, PF, CA, and L improved the egg weight and hen day egg production compared to the control group (p < 0.05). The VE, PF, and L groups significantly (p < 0.05) reduced the malondialdehyde (MDA) and maintained the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) in the egg yolk. The albumen height and Haugh unit were maintained in the egg yolk till 35 days of storage by the VE, PF, and L groups, while the CA group reduced the albumen quality after 21 d storage. The VE, PF, CA, and lutein maintained the content of alpha-linolenic acid (ALA), during the whole storage period. The total n-3 FA and docosahexaenoic acid (DHA) were retained in the egg yolk till 35 and 28 days of storage, respectively, and slightly decreased after 35 and 28 days in the L groups. The total n-6 (Tn-6) FA was maintained in the yolk till 28 days of storage in the CA and PF groups, respectively. The VE, PF, and L groups upregulated the expression of Nrf-2, P38MAPK, HO-1, SOD-1, and GSH-Px as compared to the CA and control groups. The VE, PF, and L groups significantly increased the magnum primary folds and epithelium height as compared to CA and the control. Thus, it was concluded that the use of PF and L is better at preventing egg quality deterioration and lipid oxidation, maintaining more than 300 mg/egg n-3 FA during storage, by activating the Nrf-2 pathway through the phosphorylation of P38MAPK, and enhancing the phase-2 antioxidant defense enzymes, namely, SOD, GSH-Px, and HO-1.
Intake of Sugar Substitute Gummy Candies Benefits the Glycemic Response in Healthy Adults: A Prospective Crossover Clinical Trial
Authors: Gan Dan , Xu Minjun , Chen Ling , Cui Shaohua , Deng Changyong , Qiao Qian , Guan Ruimiao , Zhong Fang
Published: October 2022

Sugar reduction in food has attracted great health concerns worldwide. Gummies have been one of the most popular and highly favored candies due to their chewable properties, simplicity to swallow, and delicious taste. The general perception is that gummies raise blood sugar levels, but the truth is that gummies with the right formula can control glycemic response. The purpose of this study is to investigate the effects of the gummy dosage form and sugar types on the glycemic response control. Maltitol and erythritol as sweetener alternatives were applied in gummy candies (total and partial sugar substitutes gummy, T-SG and P-SG), with sucrose-based gummies used as comparisons (CG). A prospective crossover study was then conducted on 17 healthy adults. The effects of different types of gummies on glycemic response in healthy adults were evaluated on the basis of the participants’ glycemic index (GI) and glycemic load (GL) values. Every three-day interval, participants took CG, P-SG, T-SG, and glucose solution, respectively, and the theoretical glucose conversion content was kept the same in all groups for each trial. Each participant performed four tests with each sample and recorded the changes in blood glucose after food consumption. It was found that all three types of gummies slowed down subjects’ glycemic response when not taken in excess, and the improvement effect was in the trend of T-SG > P-SG > CG. Both P-SG and T-SG were low-GI candies (54.1 and 49.9). CG that was not consumed in excess of 17.2 g had a high GI (81.9) but a low GL (<10). Texture analysis and in vitro digestion were used to explore the effect of gummy matrix on glucose release. T-SG and P-SG retained a higher hardness and were less hydrolyzed to release glucose during digestion compared with CG. Additionally, experiments have revealed that gummies can reverse the poor glucose tolerance in women. In conclusion, gummies are a good carrier for dietary supplements due to their sustained-release characteristic of available carbohydrates and provide healthier options for people in control of glucose homeostasis.
A First Simulation of the Impact upon the Hidroagoyán Dam Due to Lahars Triggered by an 1877-Type Cotopaxi Eruption in Ecuador
Authors: Chidichimo Francesco , Catelan Paolo , Lupiano Valeria , Straface Salvatore , Gregorio Salvatore Di
Published: October 2022

We forecast the impact that the lahars triggered on the summit of the Cotopaxi volcano in Ecuador would have upon the Hidroagoyán Dam should an 1877-type catastrophic eruption occur nowadays, with disastrous implications for the energy production of Ecuador. The Cotopaxi’ lahars have been simulated with the use of different computational models, yet none of them were so extended as to map their entire path to the dam. To fill this gap, we applied a version of the semi-empirical Cellular Automata LLUNPIY model to simulate primary and secondary lahars flowing from the summit of the Cotopaxi volcano until they reach the Hidroagoyán Dam in Baños. This version of LLUNPIY accounts for the triggering event by pyroclastic bombs and has already been validated by its successful simulation of the northbound 1877 cataclysmic lahars of the Cotopaxi volcano. The likely consequences of a similar disaster are discussed considering present territorial conditions. Computer simulations of natural hazards of this type represent a powerful tool that can be used when planning for the mitigation of environmental and social risks.
Frontline Involvement in Population COVID-19 Vaccinations: Lived Experience of Nursing Students
Authors: Villa Giulia , Galli Emanuele , Allieri Sara , Baldrighi Riccardo , Brunetti Adelaide , Giannetta Noemi , Manara Duilio Fiorenzo
Published: October 2022

(1) Background: The globally promoted vaccination campaign has been shown to be the solution for the COVID-19 pandemic, reducing transmission, hospitalisation and the need for intensive care. Although several studies have examined the experiences of healthcare workers during the pandemic, few studies have investigated healthcare student experiences. The aim of this study is to explore the lived experience of third-year nursing students during the COVID-19 vaccination campaign. (2) Methods: A phenomenological qualitative method was adopted. The researchers selected third-year students undertaking a bachelor’s nursing degree who took part in the COVID-19 vaccination campaign at a high-volume vaccination centre in the period from May to August 2021. (3) Results: Thirteen students were included in the study. Three themes and twelve subthemes emerged from the study. The themes were: a challenging experience; it is not as easy as it seems; a learning experience worth living; and teamwork and trust leading to professional development. (4) Conclusions: Participation in the vaccination campaign was a novelty for students in their degree program. Students emphasized the positive aspects of having the opportunity to participate in the vaccination campaign and help the entire community in the fight against COVID-19.
Regional Anesthetic and Analgesic Techniques for Clavicle Fractures and Clavicle Surgeries: Part 2—A Retrospective Study
Authors: Lee Chang Chuan Melvin , Lua Chong Boon , Peng Kailing , Beh Zhi Yuen , Fathil Shahridan Mohd , Hou Jin-De , Lin Jui-An
Published: October 2022

Objective. Clavicle fracture fixation is commonly performed under general anesthesia due to the complex sensory innervation in this region which poses a challenge for anesthesiologists applying regional anesthetic (RA) techniques. In part 1 of this two-part study, we summarized the current literature describing various RA approaches in clavicle fractures and surgery. In our earlier scoping review, we surmised that a superficial or intermediate cervical plexus block (CPB) may provide analgesia for this procedure and, when combined with an interscalene brachial plexus block (ISB), can provide anesthesia to the clavicular region for surgical fixation. We performed a retrospective study, consolidating assumptions that were based on the results of our earlier scoping review. Methods. A retrospective study was conducted on 168 consecutive patients who underwent clavicle fixation surgery at a tertiary healthcare system in Singapore. We used a standardized pro forma to collate perioperative data from the electronic health records of both hospitals, including anesthetic technique, analgesic requirements, pain scores, and adverse events, up to the second postoperative day or up until discharge. Results. In our study, patients who received RA had significantly reduced pain scores and opioid requirements, compared to general anesthesia (GA) alone. Through subgroup analysis, differences were found in postoperative pain scores and opioid requirements in the following order: GA alone > GA with local infiltration analgesia > CPB > CPB plus ISB. All patients who received combined CPB and ISB had upper limb weakness in recovery, compared to none with CPB alone (p < 0.001). Of those who received an ISB either in isolation or combined with a CPB, four (9.3%) were reported to have dyspnea (within 24 h) and motor weakness that persisted beyond 12 h, compared to none for patients that received CPB alone. Conclusions. Addition of a CPB to GA for clavicle fracture fixation surgery is associated with reduced pain scores in the early postoperative period, with a lower opioid requirement compared to GA alone. In patients undergoing GA, the combination of a CPB with an ISB was associated with a small, although statistically significant, reduction in pain scores and opioid requirements compared to a CPB alone.
Social Support Mediates the Association between Health Anxiety and Quality of Life: Findings from a Cross-Sectional Study
Authors: Ciułkowicz Marta , Misiak Błażej , Szcześniak Dorota , Grzebieluch Jolanta , Maciaszek Julian , Rymaszewska Joanna
Published: October 2022

This study aimed to test if perceived social support and cyberchondria mediate the association between health anxiety and quality of life (QoL) in a nonclinical sample. Cross-sectional research involved adult internet users (n = 538) between 16 May 2020 and 29 December 2020 in Poland who completed self-report questionnaires, including the cyberchondria severity scale (CSS-PL), the short health anxiety inventory (SHAI), the multidimensional scale of perceived social support (MSPSS) and the quality of life scale (QOLS). A mediation analysis was performed to examine the direct effects of health anxiety on cyberchondria, perceived social support and quality of life. Likewise, the effects of cyberchondria and perceived social support on QoL were analyzed. Hence, indirect effects of health anxiety on QoL through cyberchondria and perceived social support were explored. Health anxiety significantly impaired QoL both directly and indirectly through low-perceived social support. Perceived social support partly mediated the association between health anxiety and QoL. Cyberchondria did not have a significant direct effect on the latter. Thus, cyberchondria did not mediate the relationship between health anxiety and QoL. Boosting-perceived social support may mitigate the detrimental effect of health anxiety on QoL. Cyberchondria was not found to have a significant effect on QoL in contrast to health anxiety alone.
The Digital Divide Is Aging: An Intergenerational Investigation of Social Media Engagement in China
Authors: Zhou Yuqiong , He Tao , Lin Feng
Published: October 2022

The aging-based digital divide has gained increased research attention in recent years. This research focused on two important aspects of the aging society, the digital divide and the intergenerational differences, with regard to social media usage in China. The data were collected through a household interview, using a nationally representative sample (n= 3790) from 58 cities in mainland China. The present study investigated the association between differentiated social media usage with demographic characteristics, social economic status, physical and mental health, and social media perceptions. The results show a significant digital divide and generational differences. The existing socio-economic inequalities and demographic variance largely explained older adults’ social media access and diversity of social media engagement. Social media perceptions play more essential roles in the intensity of social media engagement for each generation group. This study contributes a better understanding of the manner in which different factors are associated with different generations’ social media engagement. A discussion is included regarding the necessity to close the aging-based digital gap in order to cope with the issue of rapid aging.
Factors Associated with the Acceptability of Mass Drug Administration for Filariasis: A Systematic Review
Authors: Abdul Halim Ahmad Farid Nazmi , Ahmad Dzulfitree , Miaw Yn Jane Ling , Masdor Noor Azreen , Ramly Nurfatehar , Othman Rahayu , Kandayah Thinakaran , Hassan Mohd Rohaizat , Dapari Rahmat
Published: October 2022

Mass drug administration (MDA) has been implemented as a tool to eliminate lymphatic filariasis. Acceptability among susceptible populations is crucial to achieving MDA effective coverage. This systematic review aims to present and systematically determine the factors associated with the acceptability of MDA. Articles related to factors associated with acceptability were collected electronically from three different databases (Scopus, Web of Science, and PubMed). Four pairs of independent reviewers screened the titles and abstracts of the collected data, stored in EndnoteX7, against the inclusion criteria. Afterwards, the included articles have been critically appraised to assess the quality of the studies using the Mixed Method Appraisal Tool (MMAT). Of the 68 articles identified, 11 were included in the final review. Knowledge, awareness, attitude and perceptions, communications, delivery and accessibility of MDA, gender, and age are the factors associated with MDA acceptability. Community acceptance remains a challenge in the implementation of MDA. To expand MDA coverage in all endemic countries, there is a strong need to address the factors influencing community acceptance of MDA.
Subjective Exercise Experience and Group Cohesion among Chinese Participating in Square Dance: A Moderated Mediation Model of Years of Participation and Gender
Authors: Ji Peiyao , Zhou Shihan , Wang Ruohang , Fan Hongying , Wang Yan
Published: October 2022

(1) Background: This study aimed to explore the relationship between years of participation, subjective exercise experience, and group cohesion among gender-specific square dance practitioners. (2) Methods: The Subjective Exercise Experience Questionnaire (SEEQ) and Group Environment Questionnaire (GEQ) were used to evaluate Subjective Exercise Experience (SEE) and group cohesion (GC). An analysis was conducted on 130 Chinese (63 males and 67 females) using multiple group analysis within a structural equation model. (3) Results: (a) The positive aspects of Subjective Exercise Experience (SEE) and Positive Well-Being (PWB), had a strongly positive effect on GC in both groups. The negative aspects of SEE, Psychological Fatigue (PF), and Psychological Distress (PD), had negative effects on GC. (b) Only for the male group was there an indirect effect of participation years on the association between SEE and GC in the model (a × b = 0.062, 95% CI [0.001, 0.181]; standard error (SE) = 0.062, p = 0.048). (c) The significant differences between paths coefficients were noticed in the association of years of participation with SEE (t = −2.043) and GC (t = −1.962). (4) Conclusion: Based on these results, gender differences in terms of the partial mediating role of adherence in the relationship of SEE and GC were presented for future research, fitness popularization, and society.
Working with Waste: Hazards and Mitigation Strategies Used by Waste Pickers in the Inner City of Durban
Authors: Mlotshwa Ntobeko , Dayaram Tanya , Khanyile Asiphile , Sibanda Princess A. , Erwin Kira , Fleetwood Tamlynn
Published: October 2022

Informal waste pickers in cities across the Global South divert significant amounts of tonnage from landfills. This diversion contributes towards a sustainable environment and better public health practices. Informal workers globally derive livelihoods from collecting, sorting, and selling recyclable waste. In South Africa, there is growing recognition of the valuable work that waste pickers carry out. Despite this, however, these informal workers remain largely unrecognised, are often stigmatised, and suffer from a lack of social protection linked to their work. This lack of recognition and protection creates specific occupational hazards for waste pickers. Using an ethnographic method, this study explores the physical and socio-psychological hazards that emerge from waste picking on the streets of the inner city of Durban, in South Africa. We found that the waste pickers, the majority of whom were women, developed mitigation strategies against these risks. A better understanding of how the occupational hazards of waste picking are shaped by the local context of working on the street enables the recognition of the knowledge waste pickers already hold regarding mitigation strategies. Insight into occupational hazards are important to consider if the municipal integration of waste pickers is to happen in a way that ensures access to social protections for these informal workers.
Transport of High-Risk Infectious Substances: Packaging for the Transport of Category A Infectious Specimens in Spain
Authors: Sánchez Jorge H. , Gouveia Susana , Cameselle Claudio
Published: October 2022

Infectious specimens and materials with pathogens included in Category A of the European Agreement concerning the International Carriage of Dangerous Goods by Road (ADR) must be transported following Packing Instruction P620. A triple packaging system must include leakproof receptacles and impact-resistant packaging to preserve the integrity of the samples and prevent the release of their content in any event during transport. ADR Packing Instruction P620 indicates that the primary receptacle or secondary packaging must withstand, without leakage, an internal pressure not less than 95 kPa at temperatures ranging from −40 °C to +55 °C. This study analyzes various packaging systems available in the Spanish market for the transportation of infectious samples to determine if they comply with the overpressure test, the most difficult to meet according to Packing Instruction P620. Five packaging systems were selected in this study. None of the secondary packaging tested showed adequate characteristics to withstand the pressure leakproof test. In this case, a primary receptacle (containing the sample directly) capable of withstanding an internal pressure of 95 kPa without leakage must be used (for example: test tubes with screw caps). However, manufacturer or distributor specifications are not always clear or readily available in this regard. Health, laboratory, and carrier personnel should be aware of the ADR regulation and packaging characteristics for safe and secure handling and transportation of high-risk Category A infectious materials.
Comparative Effectiveness of East Asian Traditional Medicine for Childhood Simple Obesity: A Systematic Review and Network Meta-Analysis
Authors: Lee Boram , Kwon Chan-Young
Published: October 2022

Childhood obesity leads to various comorbidities and usually persists into adulthood, increasing socioeconomic burden. In the absence of a clearly effective treatment, East Asian traditional medicine (EATM) therapies have been widely used. We aimed to analyze the comparative effectiveness and safety of EATM techniques for children with simple obesity through network meta-analysis (NMA). Twelve databases were searched for randomized controlled trials (RCTs) evaluating the effect of EATMs on childhood simple obesity. Individual EATMs were ranked based on the surface under the cumulative ranking curve. The risk of bias in the individual studies and publication bias in the NMA were evaluated. Thirty-three RCTs were included. Acupuncture, chuna, chuna plus acupressure, cupping plus acupressure, herbal medicine (HM), and HM plus acupuncture significantly reduced BMI compared with lifestyle management. Based on the treatment ranking, cupping plus acupressure was optimal for BMI reduction compared with a non-medical management, followed by chuna and HM. The quality of evidence for individual findings was usually moderate to low, and no serious adverse events of EATM were reported. Cupping plus acupressure might have a large beneficial effect, and chuna or HM probably have a moderate beneficial effect on reducing BMI in children with simple obesity.
The Roles of Different Fractions in Freshwater Biofilms in the Photodegradation of Methyl Orange and Bisphenol A in Aqueous Solutions
Authors: Yin Haojie , Wang Lingling , Zeng Guangshu , Wang Longfei , Li Yi
Published: October 2022

Freshwater biofilms play an important role in the migration and transformation of organic pollutants, especially under illumination conditions. Nonetheless, the roles of variable fractions in freshwater biofilms, e.g., extracellular polymeric substances (EPS), microbial cells and original biofilms, in promoting the photodegradation of trace organic pollutants remain largely unclear. In this study, two contaminants, i.e., methyl orange (MO) and bisphenol A (BPA), were selected, and the roles of different fractions in freshwater biofilms in their photodegradation performances were investigated. After dosing 696 mg/L SS biofilm harvested from an effluent-receiving river, the direct photodegradation rate of MO and BPA was increased 8.7 times and 5.6 times, respectively. River biofilm EPS contained more aromatic fractions, chromogenic groups and conjugated structures than biofilm harvested from a less eutrophic pond, which might be responsible for the enhanced photodegradation process. The quenching experiments suggested that when EPS fractions derived from river biofilm were dosed, 3EPS* was the major reactive oxygen species during the photodegradation of MO and BPA. Meanwhile, for EPS derived from the pond biofilm, ·OH/1O2 was predominantly responsible for the enhanced photodegradation. Batch experimental results suggested that the cells and EPS in river biofilms could collaboratively interact with each other to enhance the preservation of reactive species and protection of microbes, thus facilitating the photoactivity of biofilms. Our results might suggest that biofilms generated from eutrophic waterbodies, such as effluent-receiving rivers, could play a more important role in the photodegradation processes of contaminants.
The Influence of Biostimulants Used in Sustainable Agriculture for Antifungal Protection on the Chemical Composition of Winter Wheat Grain
Authors: Szpunar-Krok Ewa , Depciuch Joanna , Drygaś Barbara , Jańczak-Pieniążek Marta , Mazurek Katarzyna , Pawlak Renata
Published: October 2022

Field studies were conducted from 2016 to 2019 (south-eastern Poland; 49°58′40.6″ N 22°33′11.3″ E) with the aim to identify the chemical composition of winter wheat grain upon foliar application of biostimulants, of which PlanTonic BIO (containing nettle and willow extracts) showed antifungal activity. The main chemical compositions and their spatial distribution in wheat grain were characterized by Raman spectroscopy technique. It was established that applied biostimulants and hydro-thermal conditions changed the chemical composition of the grain during all the studied years. A similar chemical composition of the grain was achieved in plants treated with synthetic preparations, including both intensive and extensive variants. The second group, in terms of an increase in fatty acid content, consists of grains of plants treated with biostimulants PlanTonic BIO, PlanTonic BIO + Natural Crop and PlanTonic BIO + Biofol Plex. The future of using biostimulants in crop production, including those containing salicylic acid and nettle extracts, appears to be a promising alternative to synthetic crop protection products.
Effectiveness of Teaching Mini Handball through Non-Linear Pedagogy in Different Socioeconomic Contexts: A Pilot Study
Authors: Espoz-Lazo Sebastián , Farías-Valenzuela Claudio , Reyes-Contreras Victor , Ferrero-Hernández Paloma , Giakoni-Ramírez Frano , Tapia-Zavala Mauricio , Duclos-Bastías Daniel , Valdivia-Moral Pedro
Published: October 2022

Mini handball is among the sports included as part of school physical education in Chile to improve children’s motor skills and to motivate their adherence to a healthy and active lifestyle in response to concerns about this country’s high level of childhood obesity. To this end, non-linear pedagogy (NLP) has been used to develop motor skills through mini handball in the school context. However, socioeconomic differences that influence the development of children’s motor skills have not been considered to determine whether the methodology applies to everyone. The aim of the present observational study is to describe and compare the effectiveness of the previously applied NLP methodology in two contrasting socioeconomic contexts to determine whether it helps to develop motor skills through mini handball in both school contexts. The Levine test was used to determine the homogeneity of the variances (p < 0.05), as the distribution of the data was not normal. The Kruskal–Wallis H statistical test was used to analyse within-group data. Additionally, the Mann–Whitney U test was applied for comparisons between groups. The results show significant improvements in the acquisition of the expected motor skills specific to mini handball. Additionally, a shortening of the gap was evidenced between the groups during the training process, with no significant differences at the end of the progression. Therefore, the investigated NLP is equally as effective for schoolchildren in two opposite socioeconomic contexts.
NAC Transcription Factor GmNAC12 Improved Drought Stress Tolerance in Soybean
Authors: Yang Chengfeng , Huang Yanzhong , Lv Peiyun , Antwi-Boasiako Augustine , Begum Naheeda , Zhao Tuanjie , Zhao Jinming
Published: October 2022

NAC transcription factors (TFs) could regulate drought stresses in plants; however, the function of NAC TFs in soybeans remains unclear. To unravel NAC TF function, we established that GmNAC12, a NAC TF from soybean (Glycine max), was involved in the manipulation of stress tolerance. The expression of GmNAC12 was significantly upregulated more than 10-fold under drought stress and more than threefold under abscisic acid (ABA) and ethylene (ETH) treatment. In order to determine the function of GmNAC12 under drought stress conditions, we generated GmNAC12 overexpression and knockout lines. The present findings showed that under drought stress, the survival rate of GmNAC12 overexpression lines increased by more than 57% compared with wild-type plants, while the survival rate of GmNAC12 knockout lines decreased by at least 46%. Furthermore, a subcellular localisation analysis showed that the GmNAC12 protein is concentrated in the nucleus of the tobacco cell. In addition, we used a yeast two-hybrid assay to identify 185 proteins that interact with GmNAC12. Gene ontology (GO) and KEGG analysis showed that GmNAC12 interaction proteins are related to chitin, chlorophyll, ubiquitin–protein transferase, and peroxidase activity. Hence, we have inferred that GmNAC12, as a key gene, could positively regulate soybean tolerance to drought stress.
Microbiome of Nodules and Roots of Soybean and Common Bean: Searching for Differences Associated with Contrasting Performances in Symbiotic Nitrogen Fixation
Authors: Bender Flávia Raquel , Alves Leonardo Cardoso , da Silva João Fernando Marques , Ribeiro Renan Augusto , Pauli Giuliano , Nogueira Marco Antonio , Hungria Mariangela
Published: October 2022

Biological nitrogen fixation (BNF) is a key process for the N input in agriculture, with outstanding economic and environmental benefits from the replacement of chemical fertilizers. However, not all symbioses are equally effective in fixing N2, and a major example relies on the high contribution associated with the soybean (Glycine max), contrasting with the low rates reported with the common bean (Phaseolus vulgaris) crop worldwide. Understanding these differences represents a major challenge that can help to design strategies to increase the contribution of BNF, and next-generation sequencing (NGS) analyses of the nodule and root microbiomes may bring new insights to explain differential symbiotic performances. In this study, three treatments evaluated in non-sterile soil conditions were investigated in both legumes: (i) non-inoculated control; (ii) inoculated with host-compatible rhizobia; and (iii) co-inoculated with host-compatible rhizobia and Azospirillum brasilense. In the more efficient and specific symbiosis with soybean, Bradyrhizobium presented a high abundance in nodules, with further increases with inoculation. Contrarily, the abundance of the main Rhizobium symbiont was lower in common bean nodules and did not increase with inoculation, which may explain the often-reported lack of response of this legume to inoculation with elite strains. Co-inoculation with Azospirillum decreased the abundance of the host-compatible rhizobia in nodules, probably because of competitiveness among the species at the rhizosphere, but increased in root microbiomes. The results showed that several other bacteria compose the nodule microbiomes of both legumes, including nitrogen-fixing, growth-promoters, and biocontrol agents, whose contribution to plant growth deserves further investigation. Several genera of bacteria were detected in root microbiomes, and this microbial community might contribute to plant growth through a variety of microbial processes. However, massive inoculation with elite strains should be better investigated, as it may affect the root microbiome, verified by both relative abundance and diversity indices, that might impact the contribution of microbial processes to plant growth.
Sequence Characteristics and Expression Analysis of GhCIPK23 Gene in Upland Cotton (Gossypium hirsutum L.)
Authors: Chao Maoni , Dong Jie , Hu Genhai , Ren Rui , Huang Ling , Li Yanyan , Zhang Jinbao , Wang Qinglian
Published: October 2022

CIPK (calcineurin B-like-interacting protein kinase) is a kind of serine/threonine protein kinase widely existing in plants, and it plays an important role in plant growth and development and stress response. To better understand the biological functions of the GhCIPK23 gene in upland cotton, the coding sequence (CDS) of the GhCIPK23 gene was cloned in upland cotton, and its protein sequence, evolutionary relationship, subcellular localization, expression pattern and cis-acting elements in the promoter region were analyzed. Our results showed that the full-length CDS of GhCIPK23 was 1368 bp, encoding a protein with 455 amino acids. The molecular weight and isoelectric point of this protein were 50.83 KDa and 8.94, respectively. The GhCIPK23 protein contained a conserved N-terminal protein kinase domain and C-terminal regulatory domain of the CIPK gene family member. Phylogenetic tree analysis demonstrated that GhCIPK23 had a close relationship with AtCIPK23, followed by OsCIPK23, and belonged to Group A with AtCIPK23 and OsCIPK23. The subcellular localization experiment indicated that GhCIPK23 was located in the plasma membrane. Tissue expression analysis showed that GhCIPK23 had the highest expression in petals, followed by sepals, and the lowest in fibers. Stress expression analysis showed that the expression of the GhCIPK23 gene was in response to drought, salt, low-temperature and exogenous abscisic acid (ABA) treatment, and had different expression patterns under different stress conditions. Further cis-acting elements analysis showed that the GhCIPK23 promoter region had cis-acting elements in response to abiotic stress, phytohormones and light. These results established a foundation for understanding the function of GhCIPK23 and breeding varieties with high-stress tolerance in cotton.
Molecular Biomarkers for the Early Detection of Ovarian Cancer
Authors: Zhang Ruiqian , Siu Michelle K. Y. , Ngan Hextan Y. S. , Chan Karen K. L.
Published: October 2022

Ovarian cancer is the deadliest gynecological cancer, leading to over 152,000 deaths each year. A late diagnosis is the primary factor causing a poor prognosis of ovarian cancer and often occurs due to a lack of specific symptoms and effective biomarkers for an early detection. Currently, cancer antigen 125 (CA125) is the most widely used biomarker for ovarian cancer detection, but this approach is limited by a low specificity. In recent years, multimarker panels have been developed by combining molecular biomarkers such as human epididymis secretory protein 4 (HE4), ultrasound results, or menopausal status to improve the diagnostic efficacy. The risk of ovarian malignancy algorithm (ROMA), the risk of malignancy index (RMI), and OVA1 assays have also been clinically used with improved sensitivity and specificity. Ongoing investigations into novel biomarkers such as autoantibodies, ctDNAs, miRNAs, and DNA methylation signatures continue to aim to provide earlier detection methods for ovarian cancer. This paper reviews recent advancements in molecular biomarkers for the early detection of ovarian cancer.
Biomarkers of Periodontitis and Its Differential DNA Methylation and Gene Expression in Immune Cells: A Systematic Review
Authors: Cárdenas Angélica , Ardila Laura , Vernal Rolando , Melgar-Rodríguez Samanta , Hernández Hernán
Published: October 2022

The characteristic epigenetic profile of periodontitis found in peripheral leukocytes denotes its impact on systemic immunity. In fact, this profile not only stands for periodontitis as a low-grade inflammatory disease with systemic effects but also as an important source of potentially valuable clinical biomarkers of its systemic effects and susceptibility to other inflammatory conditions. Thus, we aimed to identify relevant genes tested as epigenetic systemic biomarkers in patients with periodontitis, based on the DNA methylation patterns and RNA expression profiles in peripheral immune cells. A detailed protocol was designed following the Preferred Reporting Items for Systematic Review and Meta-analysis -PRISMA guideline. Only cross-sectional and case-control studies that reported potential systemic biomarkers of periodontitis in peripheral immune cell types were included. DNA methylation was analyzed in leukocytes, and gene expression was in polymorphonuclear and mononuclear cells. Hypermethylation was found in TLR regulators genes: MAP3K7, MYD88, IL6R, RIPK2, FADD, IRAK1BP1, and PPARA in early stages of periodontitis, while advanced stages presented hypomethylation of these genes. TGFB1I1, VNN1, HLADRB4, and CXCL8 genes were differentially expressed in lymphocytes and monocytes of subjects with poorly controlled diabetes mellitus, dyslipidemia, and periodontitis in comparison with controls. The DAB2 gene was differentially overexpressed in periodontitis and dyslipidemia. Peripheral blood neutrophils in periodontitis showed differential expression in 163 genes. Periodontitis showed an increase in ceruloplasmin gene expression in polymorphonuclears in comparison with controls. Several genes highlight the role of the epigenetics of peripheral inflammatory cells in periodontitis that could be explored in blood as a source of biomarkers for routine testing.
Decreased Prosaposin and Progranulin in the Cingulate Cortex Are Associated with Schizophrenia Pathophysiology
Authors: He Yachao , Zhang Xiaoqun , Flais Ivana , Svenningsson Per
Published: October 2022

Prosaposin (PSAP) and progranulin (PGRN) are two lysosomal proteins that interact and modulate the metabolism of lipids, particularly sphingolipids. Alterations in sphingolipid metabolism have been found in schizophrenia. Genetic associations of PSAP and PGRN with schizophrenia have been reported. To further clarify the role of PSAP and PGRN in schizophrenia, we examined PSAP and PGRN levels in postmortem cingulate cortex tissue from healthy controls along with patients who had suffered from schizophrenia, bipolar disorder, or major depressive disorder. We found that PSAP and PGRN levels are reduced specifically in schizophrenia patients. To understand the role of PSAP in the cingulate cortex, we used an AAV strategy to knock down PSAP in neurons located in this region. Neuronal PSAP knockdown led to the downregulation of neuronal PGRN levels and behavioral abnormalities. Cingulate-PSAP-deficient mice exhibited increased anxiety-like behavior and impaired prepulse inhibition, as well as intact locomotion, working memory, and a depression-like state. The behavioral changes were accompanied by increased early growth response protein 1 (EGR-1) and activity-dependent cytoskeleton-associated protein (ARC) levels in the sensorimotor cortex and hippocampus, regions implicated in circuitry dysfunction in schizophrenia. In conclusion, PSAP and PGRN downregulation in the cingulate cortex is associated with schizophrenia pathophysiology.
Development of Two-Layer Hybrid Scaffolds Based on Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges for Tissue Engineering Purposes
Authors: Stocco Elena , Barbon Silvia , Zeni Elena , Cassari Leonardo , Zamuner Annj , Gloria Antonio , Russo Teresa , Boscolo-Berto Rafael , Sfriso Maria Martina , Macchi Veronica , De Caro Raffaele , Dettin Monica , Porzionato Andrea
Published: October 2022

Oxidized polyvinyl alcohol (OxPVA) is a new polymer for the fabrication of nerve conduits (NCs). Looking for OxPVA device optimization and coupling it with a natural sheath may boost bioactivity. Thus, OxPVA/chitosan sponges (ChS) as hybrid scaffolds were investigated to predict in the vivo behaviour of two-layered NCs. To encourage interaction with cells, ChS were functionalized with the self-assembling-peptide (SAP) EAK, without/with the laminin-derived sequences -IKVAV/-YIGSR. Thus, ChS and the hybrid scaffolds were characterized for mechanical properties, ultrastructure (Scanning Electron Microscopy, SEM), bioactivity, and biocompatibility. Regarding mechanical analysis, the peptide-free ChS showed the highest values of compressive modulus and maximum stress. However, among +EAK groups, ChS+EAK showed a significantly higher maximum stress than that found for ChS+EAK-IKVAV and ChS+EAK-YIGSR. Considering ultrastructure, microporous interconnections were tighter in both the OxPVA/ChS and +EAK groups than in the others; all the scaffolds induced SH-SY5Y cells’ adhesion/proliferation, with significant differences from day 7 and a higher total cell number for OxPVA/ChS+EAK scaffolds, in accordance with SEM. The scaffolds elicited only a slight inflammation after 14 days of subcutaneous implantation in Balb/c mice, proving biocompatibility. ChS porosity, EAK 3D features and neuro-friendly attitude (shared with IKVAV/YIGSR motifs) may confer to OxPVA certain bioactivity, laying the basis for future appealing NCs.
Evolutionary Origins of Metabolic Reprogramming in Cancer
Authors: García-Sancha Natalia , Corchado-Cobos Roberto , Gómez-Vecino Aurora , Jiménez-Navas Alejandro , Pérez-Baena Manuel Jesús , Blanco-Gómez Adrián , Holgado-Madruga Marina , Mao Jian-Hua , Cañueto Javier , Castillo-Lluva Sonia , Mendiburu-Eliçabe Marina , Pérez-Losada Jesús
Published: October 2022

Metabolic changes that facilitate tumor growth are one of the hallmarks of cancer. These changes are not specific to tumors but also take place during the physiological growth of tissues. Indeed, the cellular and tissue mechanisms present in the tumor have their physiological counterpart in the repair of tissue lesions and wound healing. These molecular mechanisms have been acquired during metazoan evolution, first to eliminate the infection of the tissue injury, then to enter an effective regenerative phase. Cancer itself could be considered a phenomenon of antagonistic pleiotropy of the genes involved in effective tissue repair. Cancer and tissue repair are complex traits that share many intermediate phenotypes at the molecular, cellular, and tissue levels, and all of these are integrated within a Systems Biology structure. Complex traits are influenced by a multitude of common genes, each with a weak effect. This polygenic component of complex traits is mainly unknown and so makes up part of the missing heritability. Here, we try to integrate these different perspectives from the point of view of the metabolic changes observed in cancer.
Focus on PD-1/PD-L1 as a Therapeutic Target in Ovarian Cancer
Authors: Dumitru Adrian , Dobrica Elena-Codruta , Croitoru Adina , Cretoiu Sanda Maria , Gaspar Bogdan Severus
Published: October 2022

Ovarian cancer is considered one of the most aggressive and deadliest gynecological malignancies worldwide. Unfortunately, the therapeutic methods that are considered the gold standard at this moment are associated with frequent recurrences. Survival in ovarian cancer is associated with the presence of a high number of intra tumor infiltrating lymphocytes (TILs). Therefore, immunomodulation is considered to have an important role in cancer treatment, and immune checkpoint inhibitors may be useful for restoring T cell-mediated antitumor immunity. However, the data presented in the literature until now are not sufficient to allow for the identification and selection of patients who really respond to immunotherapy among those with ovarian cancer. Although there are some studies with favorable results, more prospective trials are needed in this sense. This review focuses on the current and future perspectives of PD-1/L1 blockade in ovarian cancer and analyzes the most important immune checkpoint inhibitors used, with the aim of achieving optimal clinical outcomes. Future studies and trials are needed to maximize the efficacy of immune checkpoint blockade therapy in ovarian cancer, as well as in all cancers, in general.
Transparency Assessment on Level 2 Automated Vehicle HMIs
Authors: Liu Yuan-Cheng , Figalová Nikol , Bengler Klaus
Published: October 2022

The responsibility and role of human drivers during automated driving might change dynamically. In such cases, human-machine interface (HMI) transparency becomes crucial to facilitate driving safety, as the states of the automated vehicle have to be communicated correctly and efficiently. However, there is no standardized transparency assessment method to evaluate the understanding of human drivers toward the HMI. In this study, we defined functional transparency (FT) and, based on this definition, proposed a transparency assessment method as a preliminary step toward the objective measurement for HMI understanding. The proposed method was verified in an online survey where HMIs of different vehicle manufacturers were adopted and their transparencies assessed. Even though no significant result was found among HMI designs, FT was found to be significantly higher for participants more experienced with SAE Level 2 automated vehicles, suggesting that more experienced users understand the HMIs better. Further identification tests revealed that more icons in BMW’s and VW’s HMI designs were correctly used to evaluate the state of longitudinal and lateral control. This study provides a novel method for assessing transparency and minimizing confusion during automated driving, which could greatly assist the HMI design process in the future.
Types of Major League Baseball Broadcast Information and Their Impacts on Audience Experience
Authors: Zheng Meng-Cong , Chen Chih-Yung
Published: October 2022

Baseball is a sport that involves a large number of statistics, which are often displayed during broadcast events to show the players’ performance levels. With the advent of big data, the amount and types of data used in broadcasts have increased yearly. However, the use of complex information challenges the audience’s ability to process it. This study considered data types used during broadcasts as the basis for an in-depth exploration of audiences’ experience resulting from the application of visualization. The study also examined the relationship between the contents of broadcast information and audiences’ sports participation, entertainment experience, and cognitive load. Baseball fans with varying levels of experience with handling different types of information were surveyed to understand the variations in their entertainment experiences and cognitive load levels when they watched a baseball game. The results indicated that fans with low participation levels had insufficient viewing experience, such that the use of visualized statistical information did not facilitate their understanding of the game, nor did they gain more pleasure or meaning from the game through the visualized information. Fans with high participation levels already possessed a wealth of baseball knowledge and experience, so providing visualized information did not significantly elevate their viewing experiences either. Moreover, the visualized information caused them to experience varying amounts of additional cognitive load. These results provide a reference that can be used to design sports broadcasts tailored to different information types and fan characteristics, thus improving fans’ viewing experience of sports broadcasts.
Polarimetry for 3He Ion Beams from Laser–Plasma Interactions
Authors: Zheng Chuan , Fedorets Pavel , Engels Ralf , Kannis Chrysovalantis , Engin Ilhan , Möller Sören , Swaczyna Robert , Feilbach Herbert , Glückler Harald , Lennartz Manfred , Pfeifer Heinz , Pfennings Johannes , Schneider Claus M. , Schnitzler Norbert , Soltner Helmut , Büscher Markus
Published: October 2022

We present a compact polarimeter for 3He ions with special emphasis on the analysis of short-pulsed beams accelerated during laser–plasma interactions. We discuss the specific boundary conditions for the polarimeter, such as the properties of laser-driven ion beams, the selection of the polarization-sensitive reaction in the polarimeter, the representation of the analyzing-power contour map, the choice of the detector material used for particle identification, as well as the production procedure of the required deuterated foil-targets. The assembled polarimeter has been tested using a tandem accelerator delivering unpolarized 3He ion beams, demonstrating good performance in the few-MeV range. The statistical accuracy and the deduced figure-of-merit of the polarimetry are discussed, including the count-rate requirement and the lower limit of accuracy for beam-polarization measurements at a laser-based ion source.