bluuebunny/crossref_metadata_2025 huggingface dataset:
Features of treatment of periprosthetic hip fractures
Authors: Osipov Maxim D.
Published: November 2022

We consider periprosthetic hip fractures. The active spread of hip replacement and its large implementation into the practice of traumatology naturally lead to the fact that the number of postoperative complications is growing, which will always have high risks after this operation. The main complication is a periprosthetic fracture – bone fractures in the area of the endoprosthesis, which can be intraoperatively or at any time after surgery. This research presents the results of studies conducted at the clinical base of the traumatological and orthopedic department and the trauma center of the 1st level of the Archbishop Luke Tambov City Clinical Hospital. The purpose of the research: to study the features of periprosthetic hip fractures. Materials and methods: clinical cases of the Traumatological and Orthopedic Department and the Trauma Center of the 1st level of the Archbishop Luke Tambov City Clinical Hospital, analysis of educational and applied literature, as well as scientific articles published in journals included in the List of HAC, indexed by Scopus, RSCI, ESCI, CAS, PubMed.
Doses de calcário nos atributos químicos de solo de várzea do sul do Tocantins
Authors: Cachoeira Jader Nunes , Silva Allan Deyvid Pereira da , Leite Olavo da Costa , Santos Thiago César Nunes dos , Santos Micael Moreira , Dos Santos Cardoso Janiere
Published: November 2022

A baixa fertilidade dos solos do Cerrados é fator limitante ao desenvolvimento radicular para as principais plantas cultivadas para produção agropecuaria, promovendo baixa produtividade ha¹. O objetivo foi avaliar os efeitos de doses empregadas de Carbonato de Cálcio PA, em um Gleissolo Melânico Tb distrófico de várzea do Sul do Estado do Tocantins. Com intuito de compreender o desenvolvimento do efeito da fertilidadade dos nutrientes como: Ca, Ca+Mg, P, Mg, K e verificação da reação de pH e Al e Fe. O experimento foi desenvolvido no município de Gurupi (TO), na área experimental da Universidade Federal do Tocantins, campus de Gurupi, sendo conduzido em blocos casualizado com cincos doses de CaCO3 (0; 1,5; 3,0; 4,5 e 6,0 t ha-1), com seis repetições de 5 dm³ de solo. A cada 7 dias foram realizadas avaliações em laboratório do nível do pH em água, Al e Fe, verificando, também, os principais macronutriens. Verificou-se que a correção do solo por calcário favoreceu a elevação do pH, diminuindo os teores de Al e Fe, e aumentando os teores de P, K, Ca e Mg, Ca+Mg em solo de várzea.
Conhecimento popular do uso de plantas medicinais no município de Dom Pedrito, Rio Grande do Sul
Authors: Tejero Ignacio Pablo Traversa , Machado Fernanda Rodrigues
Published: November 2022

As plantas medicinais têm se tornado cada vez mais utilizadas na recuperação da saúde da população. Foi analisado o uso das plantas medicinais, seus fins terapêuticos e o perfil dos consumidores do município de Dom Pedrito, no Estado do Rio Grande do Sul. Os dados foram coletados por meio de um questionário estruturado. Os resultados obtidos nos retornam que o perfil da maioria dos respondentes caracteriza-se por ser do sexo feminino, com o ensino médio o grau máximo de escolarização. As espécies mais utilizadas foram Malva sylvestris, Plectranthus ornatus, Phyllanthus niruri, Casearia sylvestris, Matricaria chamomilla, Zea mays, e Mikania glomerata, sendo sua forma de obtenção mais comum por meio do próprio quintal, da família ou de vizinhos. Os principais motivos apresentados para a utilização das plantas medicinais foram a cultura, o baixo custo e baixo risco.
A Self-Emulsified Adjuvant System Containing Alpha-Tocopherol Induced Higher Neutralizing Antibody Responses than a Squalene Only Emulsion Against a Recombinant Cytomegalovirus (CMV) Pentamer Antigen
Authors: Lodaya Rushit N , Kanitkar Amey P , Ashraf Asma , Bamba Douty , Amiji Mansoor M. , O'Hagan Derek T.
Published: November 2022

Research for novel vaccine adjuvants remains a critical need to improve the immune responses to a recombinant vaccine antigen. Emulsion adjuvants such as AS03 and MF59, particularly in the area of Influenza vaccines, have shown antigen dose sparing and allowed reduced immunizations. It was previously demonstrated that these emulsion adjuvants can be formulated using a simpler, low shear process of self-emulsification. The role of alpha tocopherol as an immunomodulator in emulsion adjuvants is evident from the success of AS03 in the on-going covid-19 pandemic. Although, it was a challenge to formulate alpha tocopherol with a low shear process to get closer to the AS03 composition, the self-emulsified version referred as self-emulsifying adjuvant systems (SE-AS) showed comparable immune responses to AS03 when co-administered with a Quadrivalent influenza virus (QIV) vaccine. In this paper, we first optimized the SE-AS with alpha tocopherol referred to as SE-AS44 to allow sterile filtration. We compared the in vitro cytokine profile with self-emulsifying adjuvant 160 (SEA160), a squalene-only self-emulsified adjuvant with composition similar to MF59. We compared SE-AS44 and SEA160 co-administered with a recombinant cytomegalovirus (CMV) pentamer antigen, which is a less immunogenic antigen, in vivo to compare the antibody and T-cell responses, in different adjuvanted groups, in C57BL/6 mice.
Gradient-Applied Weighted Loss for Details of 3D Shape in Single-View Reconstruction
Authors: Lee Jiho , Kim Taehyeon , Lee Gihwan , Choe Yoonsik
Published: November 2022

There has been considerable research on reconstructing 3D shapes from single-view images; however, preserving the detailed information of the input image remains difficult. In this paper, we propose the application of a gradient map to train a network, aimed at improving the visual quality of fine-grained details such as the thin and tiny components of generated shapes. Each gradient map was created from the original voxel data, and each value represented the amount of information per volume. Here, the gradient map was defined by several methods that mathematically quantify and represent the detailed structure of an object. By applying this map to the loss function in training, we could induce the network to intensively train partial details, such as thin and narrow parts. We demonstrated that the detailed information was well-recovered when a weight that is proportional to the gradient value was applied to the loss. Furthermore, it is expected that our method will contribute to the development of 3D technologies related to the construction of virtual space for simulation and new customer experience.
Machine Learning Applied To LoRaWAN Network for Improving Fingerprint Localization Accuracy in Dense Urban Areas
Authors: Piroddi Andrea , Torregiani Maurizio
Published: November 2022

In the field of low power wireless networks, one of the techniques on which many researchers are putting their efforts is related to positioning methodologies such as fingerprinting in dense urban areas. This paper presents an experimental study aimed at quantifying the mean location estimation error in densely urbanized areas.Using a dataset made available by the University of Antwerp, a neural network was implemented with the aim of providing the position of the end-devices. In this way it was possible to measure the mean location estimation error in an area with high urban density. The results obtained show an accuracy in the localization of the end-device of less than 150 meters.This result would make it possible to use the fingerprint instead of alternative, energy consuming, methodologies such as GPS in IoT (Internet of Things) applications where battery life is the primary requirement to be met.
New Therapeutic Applications of Ozenoxacin in Superficial Skin Infections in Geriatric Patients: A Case Series
Authors: Esquirol Caussa Jordi , Sánchez Aldeguer Josep , Herrero Vila Elisabeth , Antón Gorostidi Iñaki , Colomer Giner Miquel , Olivé Torralba Anna , Abella Grau Josep Mª , Jiménez Herreros Sònia , Llobregat Delgado Carlos
Published: November 2022

INTRODUCTION: Superficial cutaneous bacterial infections have a high incidence in geriatric patients. The most implicated pathogens are gram-positive cocci (Staphylococcus aureus, Streptococcus pyogenes) while gram-negative germs are also implicated. Resistances to common topical antibiotics (mupirocin, fusidic acid) require alternatives to gram-positive and gram-negative microorganisms.Ozenoxacin cream for topical use (non-fluorinated bactericidal quinolone), in other countries and with other galenics, is indicated in children older than 6 months and in adults as a treatment of superficial bacterial infections, such as acne. In Spain, ozenoxacin cream is indicated only for non-bullous impetigo; scientific evidence show effectiveness also in other superficial skin bacterial infections.A cases series of clinical use of ozenoxacin in bacterial superficial skin infections in geriatric patients (institutionalized or community dwelling) is presented.METHODS: Multicenter case series (March-August 2022) of bacterial superficial skin infections treated with ozenoxacin cream (10mg/g every 12h, 5days); data from medical records (controls: 1-3-5 days), after obtaining informed consent (use of data and images).RESULTS: Series of 28 patients (mean age: 84,79) from nine nursing homes and one outpatient geriatric service, including acute and subacute/chronic cases.In all cases treatment was ozenoxacin 10mg/g topical cream applied every 12 hours for 5 days according to medical prescription (except for one case in which 3 days were enough for complete healing and another case treated for 10 days).Results showed complete healing in all 20 acute cases and significant clinical improvement in all subacute/chronic cases (with complete healing in one of them). Professionals scored the effectiveness in acute cases as a mean 4.5 points (maximum score: 5, p<.0001) and in subacute/chronic cases as 3.8 points (p=.010).There was no skin irritation or other adverse effects in anyone of the patients, and clinical improvement of pain, itching and other symptoms was observed, suggesting an anti-inflammatory effect. DISCUSSION AND CONCLUSIONS: Our results seem to demonstrate the effectiveness and tolerability of ozenozacin cream in bacterial infections other than non-bullous impetigo. Ozenoxacin cream is indicated only for the treatment of non-bullous impetigo; however, it is also shown to be effective, both in the scientific evidence and in our case series, for other superficial bacterial skin infections, both acute and subacute/chronic, suggesting the opportunity for clinical studies with an experimental design to evaluate the findings of clinical practice and to be able to have a therapeutic alternative to compensate for the complications of the appearance of resistance.
Discovery of the First Blattinopsids of the Genus Glaphyrophlebia Handlirsch, 1906 (Paoliida: Blattinopsidae) in the Upper Carboniferous of Southern France and Spain and Hypothesis on the Diversification of the Family
Authors: Nel André , Garrouste Romain E. , Peñalver Enrique , Hernández-Orúe Antonio , Jouault Corentin
Published: November 2022

Glaphyrophlebia victoriensis sp. nov. (Paoliida: Blattinopsidae) is the third Gzhelian representative of the genus and is described based on a beautiful forewing from the Var department in Southern France. Together with the description of another forewing fragment of a Glaphyrophlebia sp. from the Province of León in NW Spain, they improve our knowledge of fossil insects from French and Spanish late Carboniferous deposits. The specimen of Glaphyrophlebia sp. is the first mention of the family in the Carboniferous of Spain and extends the geographical distribution of the genus. These descriptions suggest that the genus Glaphyrophlebia was speciose during the Upper Pennsylvanian, while otherwise, very diverse in the early and middle Permian strata of the Russian Federation. We proposed the first hypothesis to explain the diversification of family and of its most speciose genera, and argue their diversity dynamics were likely linked with the major environmental changes that followed the collapse of the Carboniferous rainforest notably the extension of arid biomes during the Permian period. The exquisite preservation and the fineness of the sediment from Tante Victoire, in which the new species was found, suggests that the locality is suitable for preserving other fossil insects and will require additional investigations.
Compounds Characterization of a Mucuna Seed Extract: L-Dopa, Arginine, Stizolamine, and Some Fructooligosaccharides
Authors: Hernández-Orihuela Ana Lilia , Castro-Cerritos Karla Viridiana , López Mercedes Guadalupe , Martínez-Antonio Agustino
Published: November 2022

Human societies demand sustainable alternatives for goods and services. Plants are sustainable sources of important metabolites with beneficial impacts on human health. There are many reported methodologies and commercial suppliers for extract preparations from the plant Mucuna sp. They usually claim to be enriched in L-dopa, their distinctive metabolite. However, there are poor characterizations of the metabolite's components in that extracts. Here, we present the metabolite characterization of a Mucuna seed extract, emphasizing the L-dopa identification and quantification. To obtain the extracts, we follow a green and sustainable extraction protocol. The lyophilized extract was subject to liquid chromatography and mass spectrometry to identify its primary metabolites. Additionally, we follow thin-layer chromatography to identify some carbohydrates in the sample. The resultant extract has a 56% L-dopa. Other main components in the extract were arginine, stizolamine, and the fructooligosaccharides sucrose and nystose. The characterized Mucuna extract can be easily standardized as powder presentation and used in several biomedical applications.
A Mini Review of Novel Topoisomerase II Inhibitors as Future Anticancer Agents
Authors: Okoro Cosmas Obi , Fatoki Toluwase Hezekiah
Published: November 2022

Several reviews of inhibitors of topoisomerase II literature have been published covering research before 2018. Therefore, this review is focused primarily on more recent publications with relevant points from the earlier literature. Topoisomerase II is an established target for anticancer drugs, that are further subdivided into poisons and catalytic inhibitors. Whereas most of the topoisomerase II-based drugs in clinical use are mostly topoisomerase II poisons, their mechanism of action has posed severe concern due to DNA damaging potential, including development of multi drug resistance. As a result, we are beginning to see a gradual paradigm shift towards a non-DNA damaging agents, such as the lesser studied topoisomerase II catalytic inhibitors. In addition, this review will describe some novel selective catalytic topoisomerase II inhibitors. The ultimate goal is to bring researchers up to speed by curating and delineating new scaffolds as leads for optimization and development to new potent, safe and selective agents for the treatment of cancer.
Reinserción social e invisibilidad
Authors: Bazán Deirdré
Published: November 2022

Se presenta un panorama que aborda la indiferencia o poco interés social por las personas privadas de la libertad y sus procesos de reinserción; esto, desde los ámbitos normativos, académicos, empresariales, sociales y familiares. Por ello, de manera propositiva, se enuncian cuatro planteamientos que abonarían a la profundización del análisis y reflexión sobre el tema y que pudieran servir como base para acabar con la indiferencia gubernamental e invisibilidad social.
De la reintegración a la amnistía
Authors: Vázquez Martínez Alejandro Ernesto
Published: November 2022

Analiza la correlación entre las políticas de reintegración social y la propuesta de pacificación anunciada a principios del actual gobierno federal, subrayando las contradicciones y retos a las que nos enfrentamos como ciudadanía, así como la necesidad de cuestionarnos acerca de nuestros supuestos, saberes y prejuicios.
Limitaciones en los proyectos para educación básica de la SEP durante pandemia
Authors: Gallegos de Dios Osbaldo Amauri
Published: November 2022

En el artículo se analizan las problemáticas de la población mexicana al usar el aprendizaje por televisión a causa de la pandemia del Covid-19. De esta forma, se puede entender que si con las clases en línea se considera que los estudiantes no estarán preparados para el siguiente escolar (2021-2022), con las clases basadas en el aprendizaje por televisión el panorama fue muy desalentador en México y la respuesta educativa de la SEP tuvo grandes deficiencias durante la pandemia.
Fidel Castro, ¿héroe mitológico?
Authors: O´farrill Jiménez Juan Carlos
Published: November 2022

La última vez que vi a Fidel Castro no semejaba siquiera la sombra de un héroe. No vestía su uniforme verde olivo. No estaba de pie. Su voz no era firme sino un murmullo que se apaga por instantes y que serpentea como un tímido arroyo en tiempos de sequía. El invicto comandante en jefe, aquella tarde de abril de 2016, no era el hombre con que las jóvenes cubanas habían fantaseado a finales de la década de 1950. Mucho menos el guerrero armado y sonriente que mi padre, desde la perplejidad de su infancia, acudió a ver desfilar triunfante en enero de 1959. Parecía más bien un triste dictador que, a punto de morir, quiere alterar el modo en que van a recordarle. Creo que, justamente, eso era.
Auge y Caída del Tractatus Logico-Philosophicus
Authors: Tomasini Bassols Alejandro
Published: November 2022

Lo que sucede es que Wittgenstein fue siempre un pensador original, es decir, no asimilable a ninguna corriente y, por consiguiente, un pensador poco proclive a servirse de terminologías acuñadas por otros.
Implementación de un servicio intranet cloud para la mejora en la gestión del factoring en la empresa Plus capital Perú SAC, 2021
Authors:   , Torres Mejia Brigitte Stefani
Published: November 2022

El objetivo de la investigación es determinar la implementación de un servicio intranet cloud en la gestión del Factoring en la empresa Plus Capital SAC, debido a la ausencia de una intranet que permita acceder a sus archivos e ingresos de operaciones desde cualquier dispositivo a través del internet. La investigación tiene enfoque cuantitativo, método hipotético deductivo, tipo aplicada y de diseño Pre Experimental por ser un solo grupo de control y un cuestionario validado por expertos. La muestra estuvo conformada por el total de colaboradores. Los resultados nos muestran que la implementación de un servicio intranet cloud influye significativamente en la mejora de la gestión del Factoring en la compañía analizada. Se obtuvo el objetivo general de la investigación, considerando que la gestión del Factoring mejoró al implementarse un sistema de intranet cloud mediante la prueba T para muestras emparejadas con sig<0.001, lo que hizo una prueba significativa.
Hábitos de consumo y comercio electrónico en los millenials de Lima Metropolitana durante la pandemia COVID-19/2021
Authors:   , Angulo Espinoza Vanessa Estefania , Mongrut Tello Patricia del Pilar
Published: November 2022

Desde el punto de vista de este estudio, el objetivo de la investigación considera “determinar si los hábitos de consumo se relacionan con el comercio electrónico en los millennials de Lima Metropolitana durante la pandemia Covid -19/2021”. Respecto a la metodología, se desarrolló el diseño no experimental, de enfoque cuantitativo, alcance descriptivo correlacional. La muestra fue compuesta de 384 personas entre los 23 a 37 años (millennials). Para la obtención de datos se aplicó un cuestionario conformado por 28 ítems para ambas variables. Se trabajó con la prueba de normalidad de Rho de Spearman, encontrando correlaciones positivas. Para la hipótesis general la correlación hallada fue de (r = 0,371) indicando que la relación es baja, concluyendo que los hábitos de consumo se relacionan significativamente con el comercio electrónico en los millennials de Lima Metropolitana durante la pandemia Covid-19/2021.
Characterization of Nonlinear Viscoelastic Properties of Enhanced Oil Recovery Polymer Systems Using Steady-Shear Rheometry
Authors: Azad Madhar Sahib
Published: November 2022

Enhanced oil recovery (EOR) polymer systems such as hydrolyzed polyacrylamide (HPAM) solutions flowing in porous media at high fluxes were reported to cause shear-thickening, a nonlinear viscoelastic (NLVE) phenomenon. Bulk rheological studies are usually performed to characterize the viscous and viscoelastic behaviors of polymer solutions in porous media. To characterize the viscoelastic properties, oscillatory shear rheology based on linear viscoelastic (LVE) data and extensional rheology based on NLVE data have been used. Although both extensional flow and high-speed shear flow can stretch and thicken polymer solutions in a nonlinear fashion, steady-shear rheometry has been used to measure only the viscous and thinning behavior of HPAM systems. In this paper, (a) the thickening ability of HPAM polymer systems formulated with contrasting salinity and molecular weight (MW) concentration is characterized in bulk shear rheometry to analyze if the thickening in the pure shear field correlates with the thickening behavior reported in porous media and other rheometry and (b) the thickening intensity of the polymer solutions of varying salinity concentration in the bulk shear field is compared with the reported mechanical degradation effects in the literature.

The shear-thickening index obtained by fitting a power-law model to thickening data in steady-shear rheograms is higher for the high-saline, high-MW low-concentration HPAM systems that have inherently higher nonlinear viscoelasticity. Furthermore, the thickening behavior observed due to salinity variation in bulk steady shear contrasts with linear oscillatory shear behavior but conforms to the thickening behavior observed in porous media and in the extensional field. This signifies that polymer-EOR researchers performing comparative studies on low-salinity and high-salinity polymer floods, and having a shear rheometer at their disposal, must look beyond linear oscillatory rheology and make use of nonlinear steady-shear rheology. There is a direct relation between the shear-thickening index of saline HPAM solutions and their mechanical degradation intensity. This paper shows that the high-speed data in steady-shear rheometry that are usually ignored in EOR literature have useful information and the notion that HPAM solutions are purely thinning in the bulk shear field needs to be reconsidered.
Peripheral blood CD86-positive plasmacytoid dendritic cells and immune-related adverse events in lung cancer patients
Authors: Karayama Masato , Mizoguchi Yukihiro , Inoue Yusuke , Hozumi Hironao , Suzuki Yuzo , Furuhashi Kazuki , Fujisawa Tomoyuki , Enomoto Noriyuki , Nakamura Yutaro , Inui Naoki , Suda Takafumi , Kitano Shigehisa , Aoki Kazunori , Yamada Yasuhide
Published: November 2022

Background: The immune-related adverse events (irAEs) is unpredictable. Peripheral blood mononuclear cell (PBMC) profiles have potential for predicting irAEs.
Methods: PBMCs were measured using multicolor flow cytometry before and at weeks 2 and 8 after the immune checkpoint inhibitors (ICIs) in lung cancere patients.
Results: Sixteen patients were eligible for the analysis. The irAEs occurred in 6 (37.5%) patients. Patients with irAEs demonstrated higher levels of CD86+plasmacytoid dendritic cells (pDCs) at baseline and weeks 2 and 8 after the ICIs than patients without irAEs (p = 0.005, 0.038, and 0.050, respectively). Higher levels of natural killer (NK) cells were significantly associated with overall objective response (p = 0.024).
Conclusions: Higher levels of CD86+pDCs at baseline and the reduction at 2 and 8 weeks after the ICIs were associated with the occurrence of irAEs. Higher levels of NK cells were associated with therapeutic efficacy. Measurements of PBMCs may be useful for predicting the efficacy and safety of ICIs.
Exosomal circRNA-miRNA Expression profile from plasma in Alzheimer’s Disease Patients by Bioinformatics and Integrative Analysis
Authors: Besli Nail , Sarikamis Bahar , Cakmak Rabia Kalkan , Kilic Ulkan
Published: November 2022

Background Alzheimer's disease (AD) is an age-dependent neurodegenerative ailment globally. Compelling evidence suggests the function of exosomal non-coding RNAs has been associated with the progression of AD but whose exosomal-linked non-coding RNAs mediated regulatory mechanisms are broadly unlit. This study, therefore, set out with the aim of exploring the exosomal circRNA-miRNA networks in the plasma of AD patients.
Methods and Results Data of 3 samples from each group (healthy, mild cognitive impairment (MCI), and AD) were fetched from ArrayExpress. The MCI and AD groups were compared with the healthy group by screening for differentially expressed miRNAs (DEmiRs) and circRNAs (DEcircRs) in plasma exosomes. Subsequently, common DEmiRs and DEcircRs for both MCI and AD groups were evaluated to identify gene ontologies, pathways, and networks. Lastly, the analysis of the PPI (protein–protein interaction) network and hub genes selection were performed. A total of common 19 (7 upregulated and 12 downregulated) DEmiRs and 24 DEcircRs were identified. It was predicted 4559 target genes for upregulated DemiRs, while 6504 target genes for downregulated DEmiRs and most of the target genes were associated with the PI3K-Akt pathway and that they were mostly regulated by hsa-mir-615-3p, hsa-mir-196a-5p, hsa-let-7c-5p, hsa-let-205-5p, hsa-mir-185-3p, hsa-mir-185-5p, hsa-mir-374a-5p, hsa-mir-374a-3p. Also, 9 hub genes (CCNE2, CCND1, CDK6, ACTB, MAPK1, AKT1, GSK3B, IGF1R, HSP90AA) were uncovered as the genes most associated with AD by a PPI network using Cytoscape plug-in cytohubba.
Conclusions Our outcomes exhibit a new outlook on a possible exosomal-linked miRNA-circRNA network in the pathogenesis of AD.
C/D box small nucleolar RNA SNORD104 promotes endometrial cancer by regulating the 2'-O-methylation of PARP1
Authors: Lu Bingfeng , Chen Xi , Liu Xin , Chen Jingwen , Qin Honglei , Chen Shuo , Zhao Yang
Published: November 2022

Background: Small nucleolar RNAs (snoRNAs) are dysregulated in many cancers, although their exact role in tumor genesis and progression remains unclear.
Methods: The expression profiles of snoRNAs in endometrial cancer (EC) tissues were analyzed using data from The Cancer Genome Atlas, and SNORD104 was identified as an upregulated snoRNA in EC. The tumorigenic role of SNORD104 in EC was established in CCK8, colony formation, EdU, apoptosis, Transwell, and in vivo xenograft experiments. The molecular mechanisms of SNORD104 were analyzed by RNA immunoprecipitation (RIP), Nm-seq, RTL-P assay, RNA stability assay, qRT-PCR, and western blotting.
Results: Antisense oligonucleotide (ASO)‑mediated knockdown of SNORD104 in Ishikawa cells significantly inhibited their proliferation, colony formation ability, migration, and invasion in vitro and increased apoptosis. On the other hand, overexpression of SNORD104 promoted EC growth in vivo and in vitro. RIP assay showed that SNORD104 binds to the 2'-O-methyltransferase fibrillarin (FBL), and according to the results of Nm-seq and RTL-P assay, SNORD104 upregulated PARP1 (encoding poly (ADP-ribose) polymerase 1) 2'-O-methylation. The binding of FBL to PARP1 mRNA was also verified by ROP assay. Furthermore, SNORD104 expression was positively correlated with PARP1 expression in EC tissues. In the presence of actinomycin D, SNORD104 increased the stability of PARP1 mRNA and promoted its nuclear localization. Finally, silencing FBL or PARP1 in the HEC1B cells overexpressing SNORD104 inhibited their proliferative and clonal capacities and increased apoptosis rates.
Conclusions: SNORD104 enhances PARP1 mRNA stability and translation in the EC cells by upregulating 2'‑O‑methylation and promotes tumor growth.
Validity of the modified Rankin Scale in patients with aneurysmal subarachnoid hemorrhage: a randomized study
Authors: Nobels-Janssen E , Postma E N , Abma I L , Dijk J M C van , de Ridder I R , Schenck H , Moojen W A , Hertog M H den , Nanda D , Potgieser A R E , Coert B A , Verhagen W I M , Bartels R H M A , Wees P J van der , Verbaan D , Boogaarts H D
Published: November 2022

Purpose
The modified Rankin Scale (mRS), a clinician-reported outcome measure of global disability, has never been validated in patients with aneurysmal subarachnoid hemorrhage (aSAH). The aims of this study are to assess: 1) convergent validity of the mRS; 2) responsiveness of the mRS; and 3) the distribution of mRS scores across patient reported outcome measures (PROMs).
Methods
This is a prospective, randomized, multicenter study. The mRS was scored by a physician for all patients; subsequently for one half by structured interview and the other half by self-assessment. All patients completed EuroQoL 5D-5L, RAND-36, Stroke Specific Quality of Life scale (SS-QoL) and Global Perceived Effect (GPE). Convergent validity and responsiveness were assessed by testing hypotheses.
Results
In total, 149 patients were included for analysis. The correlation of the mRS with EQ-5D-5L was r = -0.546, with RAND-36 physical and mental component score being r = -0.439 resp. r = -0.574, and with SS-QoL r = -0.671. Three out of four hypotheses for convergent validity were met. The mRS assessed through structured interview has higher correlation with the mental component score than with the physical component score of RAND-36. Improvement on GPE was indicated by 83% of patients; the mean change score of these patients on the mRS was − 0.08 (SD 0.915). None of the hypotheses for responsiveness were met.
Conclusion
The results show that the mRS generally correlates with other instruments as expected, but it lacks responsiveness. A structured interview of the mRS is best for detecting disabling neuropsychological complaints.
Registration
URL: https://trialsearch.who.int; Unique identifier: NL7859, Date of first administration: 08-07-2019
Barriers and facilitators to publicly-funded gender-affirming surgery: The perspectives amongst a cohort of Australian clinicians
Authors: Piñón-O’Connor Katie E. , Mullens Amy B. , Debattista Joseph , Sanders Tait , Brö Annette
Published: November 2022

Background: Barriers to publicly-funded gender-affirming surgery (GAS) in Australia have been identified as cost, limited availability of qualified providers and lack of public hospital systems performing/offering these services. Our study explores barriers, facilitators, and potential implications for expanding, and improving publicly-funded GAS in Australia from the perspectives of an Australian cohort of gender-affirming clinicians.
Methods: We conducted semi-structured interviews with eight clinicians who currently work within gender-affirming health services in Queensland, Australia. Through ecological systems theory, gender minority stress framework, and reflexive thematic analysis, themes and subthemes were identified.
Results: Our study identified three themes and five sub-themes exploring the barriers and facilitators to publicly-funded GAS in Queensland, Australia. The first theme “I don’t know how this it’s ever going to happen” captures participant concerns that gender-affirming surgery is a misunderstood intervention within the wider medical community and this perception impedes progress of publicly-funded GAS. The second theme, “Hoping Against Hope” depicts the double-edged dilemma of maintaining hope of accessing surgery even if it is “unrealistic.” The last, and third theme, “No if, When” depicts the certainty amongst participants that publicly-funded GAS is inevitable and will become a reality in Queensland, Australia.
Conclusions: Gender-affirming clinicians indicated establishing a surgical center for excellence in trans and non-binary healthcare is an essential facilitator needed to implement publicly-funded GAS. This would allow for a best-practice decentralized model of gender-affirming care to be realized in future to optimize health and wellbeing among trans and non-binary persons. Thereremain substantial barriers, specifically at exosystem and macrosystem levels, within the public health service needing urgent attention. Implications of findings are relevant to funding, clinical practice, research, and policy within and beyond Australia.
PPARγ-dependent hepatic macrophage switching acts as a central hub for hUCMSCs-mediated alleviation of decompensated liver cirrhosis in rats
Authors: Yao Yunqi , Zhang Lin , Cheng Fuyi , Jiang Qingyuan , Ye Yixin , Ren Yushuang , He Yuting , Su Dongsheng , Cheng Lin , Shi Gang , Dai Lei , Deng Hongxin
Published: November 2022

Background: Decompensated liver cirrhosis (DLC), a terminal-stage complication of liver disease, is a major cause of morbidity and mortality in patients with hepatopathie. Human umbilical cord mesenchymal stem cell (hUCMSCs) therapy has emerged as a treatment novel alternative for the treatment of DLC. However, optimized therapy protocols and the associated mechanisms are not completely understood.
Methods: We constructed a DLC rat model consistent with the typical clinical characteristics combined use of PB and CCL4. By performing dynamic detection of liver morphology and function in rats for 11 weeks, the various disease characteristics of DLC and the therapeutic effect of hUCMSCs on DLC in experimental rats were fully investigated, according to ascites examination, histopathological and related blood biochemical analyses. Flow-cytometry analysis of rat liver, immunofluorescence and RT-qPCR were performed to examine the changes of liver immune microenvironment after hucMSCs treatment. RNA-seq analysis of liver and primary macrophages and hUCMSCs co-culture system in vitro were performed to explore possible signaling pathways. PPARγ antagonist, GW9662, and clodronate liposomes were used to inhibit PPAR activation and pre-exhaustion of macrophages in DLC rats’ liver respectively.
Results: We found that changing of the two key issues, the frequency and initial phase of hUCMSCs infusion can affect the efficacy of hUCMSCs and the optimal hUCMSCs treatment schedule is once every week for three weeks at the early-stage of DLC progression, providing the best therapeutic effect in reducing mortality and ascites, and improving liver function in DLC rats. hUCMSCs treatment skewed the macrophage phenotype from M1-type to M2-type through activating PPARγ signaling pathway in liver, which was approved by primary macrophages and hUCMSCs co-culture system in vitro. Both inhibition of PPARγ activation with GW9662 and pre-exhaustion of macrophages in DLC rats’ liver abolished the regulation of hUCMSCs on macrophage polarization, thus attenuating the beneficial effect of hUCMSCs treatment in DLC rats.
Conclusions: HUCMSCs treatment on DLC were attributed to the activation of the PPARγ signaling pathway in liver macrophages of DLC rats, which polarizes M1-type macrophages to M2-type, thus inhibiting inflammation and promoting the repair of damaged liver tissue. Our results about different infusion regimens comparison and mechanisms exploration provide a robust theoretical foundation for the future study design of Mesenchymal Stem Cells therapy on DLC.
Performance Improvement of an Industrial Axial Turbine: Two-Stage Power Turbine Aerodynamic Optimization
Authors: Amini Ali Mobasher , Khavari Ali , Alizadeh Mohammad
Published: November 2022

For high-aspect-ratio turbine blades, profile loss is dominant and its reduction is important for improving performance. In this paper, a two-stage low-pressure turbine (power turbine) is optimized in two steps: cycle optimization and aerodynamic optimization. In the cycle optimization step, the thermodynamic boundary condition of the turbine is modified. In the second step, which is the main focus of this study, the aerodynamic improvement of a turbine using a two-dimensional optimization technique is performed. The Genetic Algorithm (GA) is used in the optimization process to identify variables that satisfy a defined objective function which is subject to some constraints. The Artificial Neutral Network (ANN) is introduced to correlate variables with the defined objective functions and constraints. Maximum velocity and its location on the blade surface determine transition location and diffusion factor which directly affect total pressure losses. Therefore, in the optimization process, maximum velocity and its location are controlled and defined as objective functions. Profile shape at any given radius is represented with the camber-thickness method. The flow solver used for the aerodynamic analysis of profiles is the cascade analysis code MISES. After improving the performance of vanes and blades’ 2D sections, to be confident for performance enhancement of final power turbine ANSYS CFX is used. Finally, the aerodynamic characteristics of the power turbine using optimized geometries are discussed and compared to the original one. The results demonstrate a 1.19 percent improvement in power turbine efficiency at the same pressure ratio.
Study Title Efficacy of Interventions aiming to Increase Nurse Resilience: A Rapid Review of the Literature
Authors: Mallon Anita , Mitchell Gary , Lecturer Gillian Carter , McLaughlin Derek , Wilson Christine Brown
Published: November 2022

BACKGOUND
The COVID 19 pandemic has exposed and further exacerbated systemic weaknesses in the health and social care system, placing an inordinate pressure on nurses. The importance of effective resilience building interventions as a strategy to enable recovery and growth in these times of uncertainty have been advocated. The aim of this rapid review was to inform the development of a resource to support the resilience of care home nurses by extracting empirical evidence as to the efficacy of resilience building interventions undertaken with nurses
METHODS
We undertook a rapid review using quantitative studies published in peer reviewed journals that reported resilience scores using a valid and reliable scale before and after an intervention aimed at supporting nurse resilience. The databases; Cumulative Index to Nursing and Allied Health Literature, Medline and PsychInfo. and the Cochrane Library were searched. The searches were restricted to studies published between January 2011 and October 2021 in the English language. Only studies that that reported using a validated tool to measure resilience before and after the interventions were included.
RESULTS
Fifteen studies were included in this rapid review with over half of the studies taking place in the USA. No studies reported on an intervention to support resilience with care home nurses. The interventions focused primarily on hospital-based nurses in general and specialist contexts. The interventions varied in duration content and mode of delivery, with interventions incorporating mindfulness techniques, cognitive reframing and holistic approaches to building and sustaining resilience. Thirteen of the fifteen studies selected demonstrated an increase in resilience scores as measured by validated and reliable scales. Those studies incorporating ‘on the job,’ easily accessible practices that promote self-awareness and increase sense of control reported significant differences in pre and post intervention resilience scores.
CONCLUSION
Nurses continue to face significant challenges, their capacity to face these challenges can be nurtured through interventions focused on strengthening individual resources. The content, duration, and mode of delivery of interventions to support resilience should be tailored to specific populations and responsive to their unique contexts.
Which factors affect the spontaneous stone passage after flexible ureteroscopic lithotripsy for renal stones?
Authors: Liao NaiKai , TAN ShuTing , YANG GuangLin , YANG ShuBo , ZHAI GaoQiang , LI ChengYang , Mo LinJian , CHENG JiWen
Published: November 2022

Objectives: The aim of this study was to investigate factors predicting spontaneous stone passage after flexible ureteroscopic lithotripsy ( fURS) for renal stones.
Materials and Methods: This study included 239 patients with renal stones who underwent fURS using the dusting technique. The final outcome was evaluated 3 months postoperatively and the residual fragment status (RFs) was defined as any residual stone fragments greater than 2 mm. Univariate and multivariate analyses of possible predictive factors associated with spontaneous clearance of residual renal fragments were performed.
Results:186 patients(77.8%) had achieved an stone-free status (SFs) and 53 patients(22.2%) were considered with RFs. Univariate analysis showed the stone location, stone number, stone size, stone CT value, IPA degree, the presence of hydronephrosis and stone covered with purulent substance were all associated with RFs (P<0.05). Multivariate logistic regression analysis showed that larger stone size (P=0.001),multiple stones (P=0.038) , the IPA of <45°(P=0.035) , the presence of hydronephrosis (P=0.045), the stone CT value ≥1000 (P=0.047) were all significantly associated with higher rates of residual stone after fURS. The stone covered with purulent substance was found to be the strongest predictor of RFs (P=0.027). However, the presence of lower pole stone had no significant influence on stone clearance after fURS (P=0.263).
Conclusions: Stone number, stone size , an IPA of <45°, stone CT value ≥1000 HU, stone covered with purulent substance and presence of hydronephrosis are all predictive factors of the spontaneous clearance of residual renal fragments after fURS.
Prevalence and Factors Associated with Genital Tobacco Powder Use Among Women in the Gambia: A Mixed Study
Authors: Jaiteh Musa , Awolude Olutosin A. , Bah Haddy-Tunkara
Published: November 2022

Background:Genital tobacco powder, locally known as "tabaa," is a mixture of powdered tobacco and other substances that women use vaginally for sexual enhancement. Through a media report, the Ministry of Health issued a warning for women to stop the practice. However, literature search showed that no any scientific study documenting the burden of this cultural practice beyond media reports. The aim of this study was to determine the prevalence and factors associated with genital tobacco powder use among women in The Gambia.
Methods: A sequential exploratory mixed approach was used. The research was carried out in two areas of The Gambia: one rural (Farafenni) and one urban (Banjul). Focus group discussions and in-depth interviews were used to recruit (30) participants in the qualitative study. The quantitative study interviewed (400) women using structured questionnaire that was developed from the qualitative results. SPSS version 24 and STATA version 7 were used to analyze the data. Descriptive, Bivariate and multivariate statistics were used to summarize the data.
Results: Most of the study participants were between 30-39 years old who were married. The prevalence (17.0%; n=68) of genital tobacco powder was high among women aged 40 years and above (OR = 2.88; 95% CI = 1.03–8.05; p = 0.04), from Farafenni and satellite villages (OR = 2.01; 95% CI = 1.02-3.96; p = 0.04), had non-formal education (OR = 3.09; 95% CI 0.85–11.26; p = 0.02) and had husbands who traveled for 6 (OR = 6.67; 95% CI = 1.71–26.01; p= 0.006) or 12 or more months (OR = 9.64; 95% CI 3.19-29.18; p<0.001). The perception of women and peer influence were associated with genital tobacco powder use.
Conclusion: Genital tobacco powder is practiced all over the Gambia and highest in the rural areas. Socio-demographic variables such age, residence, marital status, education status were significantly associated with the practice. According to the findings, women with low level of education and have poor perception can easily be convinced by close friends/family members to use genital tobacco powder. Therefore, awareness creation is the most important intervention to mitigate this practice.
Re-translocation of photoassimilates by Nano-TiO2 spraying in favor of osmotic adjustment in water-stressed sunflower
Authors: Ramadan Taha , Sayed Suzan A. , Abd-Elaal Amna K.A. , Amro Ahmed M.A
Published: November 2022

Background
Water deficit is one of the main environmental stresses that modifies the biomass allocation patterns between shoots and roots. Any attempt to improve the water status of plants, especially in regions of water scarcity, will be significantly important. In this study, the effect of foliar application of nanoparticles or ordinary TiO2 on water status of Helianthus annuus subjected to water deficit stress was evaluated.
Results
The water content of H. annuus shoots or roots didn’t change significantly by spraying with different concentrations of Nano- or Ord-TiO2. The dry mass (DM), relative dry mass (RDM) and root / shoot ratio of sunflower sprayed with Nano-TiO2, when averaged across all concentrations, mostly didn’t differ significantly from those sprayed with Ord-TiO2. In roots, the DM and RDM were decreased with increasing concentration of Ord-TiO2 but unchanged significantly by spraying with Nano-TiO2. Under all levels of water availability, total osmotic potential (ψs) and osmotic potential contributed by organic substances (ψorganic) didn’t change significantly by Ord-TiO2. Nano-TiO2 doesn't have any effect on the shoot or root dry mass and osmotic potential contributed by electrolytes (ψelect). Low concentrations of Nano-TiO2 significantly decreased relative water content (RWC) and ψs due to decreasing ψorganic. The ψs and ψelect of the root sap of sunflower were greatly lower than that of leaf sap. The soluble sugars partitioning and re-translocation was mainly in the priority of osmotic adjustment of the roots as a functional equilibrium under water deficit stress.
Conclusion
The foliar application of Nano-TiO2 didn’t significantly improve the sunflower water status built up by the shortage in water supply, and the quite small effect was via re-translocation of electrolytes and organic substances from shoots to roots.
Tectona grandis: Narrow Genetic Base? A New Perspective on the Genetic Variability of Teak
Authors: Anjos Isabela Vera , Gilio Thiago A. S. , Amorim Ana Flávia S. , Jesus Jeferson G. , Chimello Antonio M. , Takizawa Fausto H. , Araújo Kelly Lana , Neves Leonarda Grillo
Published: November 2022

Teak (Tectona grandis Linn. f.) is considered one of the most expensive hardwoods in the world. The dispersion of the species over the years has taken the teak beyond its origin centers and little is known about the genetic origin and genetic variability. This study aimed to investigate the genetic diversity and population structure existing in a teak germplasm bank collection in Brazil. DNA was extracted from young leaves and each sample were genotyped by whole genome sequencing at 8x of coverage, the sequencing were aligned using the genome at NCBI, and SNPcalls and quality control were made. To study the population structure of the genotypes, Bayesian variational inference was used via fastStructure, the phylogenetic tree was based on the modified Euclidean distance and the clustering by the UPGMA hierarchical method. Genetic diversity was analyzed based on the pairwise genetic divergence (Fst) of Weir and Cockerham. Genotyping by sequencing resulted in a database of approximately 1.4 million of variations SNPs were used for analysis. It was possible to identify four subpopulations with genetic variability between and within the subpopulations, so this study made it possible to confirm the existence of genetic variability in teak, contrary to what was expected.