bluuebunny/crossref_metadata_2025 huggingface dataset:
A Structural Perspective on Calprotectin as a Ligand of Receptors Mediating Inflammation and Potential Drug Target
Authors: Garcia Velia , Perera Yasiru Randika , Chazin Walter Jacob
Published: March 2022

Calprotectin, a heterodimer of S100A8 and S100A9 EF-hand calcium-binding proteins, is an integral part of the innate immune response. Calprotectin (CP) serves as a ligand for several pattern recognition cell surface receptors including the receptor for advanced glycation end products (RAGE), toll-like receptor 4 (TLR4), and cluster of differentiation 33 (CD33). The receptors initiate kinase signaling cascades that activate inflammation through the NF-kB pathway. Receptor activation by CP leads to upregulation of both receptor and ligand, a positive feedback loop associated with specific chronic inflammatory syndromes. Hence, CP and its two constituent homodimers have been viewed as potential targets to suppress certain chronic inflammation pathologies. A variety of inhibitors of CP and other S100 proteins have been investigated for more than 30 years, but no candidates have advanced significantly into clinical trials. Here, current knowledge of the interactions of CP with its receptors is reviewed along with recent progress towards the development of CP-directed chemotherapeutics.
Campylobacter jejuni Serine Protease HtrA Induces Paracellular Transmigration of Microbiota across Polarized Intestinal Epithelial Cells
Authors: Sharafutdinov Irshad , Tegtmeyer Nicole , Müsken Mathias , Backert Steffen
Published: March 2022

Campylobacter jejuni represents an eminent zoonotic germ responsible for foodborne infections causing campylobacteriosis. In addition, infections with C. jejuni constitute a risk factor for the occurrence of inflammatory bowel disease (IBD). In the latter case, patients show inflammatory reactions not only against C. jejuni, but also against the non-infectious microbiota. However, the involved mechanisms and molecular basis are still largely unclear. We recently reported that C. jejuni breaches the intestinal epithelial barrier by secretion of serine protease HtrA (high temperature requirement A), which cleaves several major tight and adherens junction proteins. In the present study, we aimed to study if HtrA-expressing C. jejuni may also trigger the transepithelial migration of non-pathogenic gastrointestinal microbiota. Using confocal immunofluorescence and scanning electron microscopy, we demonstrate that C. jejuni wild-type (wt) as well as the isogenic ∆htrA mutant bind to the surface of polarized intestinal Caco-2 epithelial cells, but do not invade them at the apical side. Instead, C. jejuni wt, but not ∆htrA mutant, disrupt the cellular junctions and transmigrate using the paracellular route between neighboring cells. Using transwell filter systems, we then co-incubated the cells with C. jejuni and non-invasive microbiota strains, either Escherichia coli or Lactococcus lactis. Interestingly, C. jejuni wt, but not ∆htrA mutant, induced the efficient transmigration of these microbiota bacteria into the basal compartment. Thus, infection of the intestinal epithelium with C. jejuni causes local opening of cellular junctions and paracellular translocation in an HtrA-dependent manner, which paves the way for transmigration of microbiota that is otherwise non-invasive. Taken together, these findings may have impacts on various Campylobacter-associated diseases such as IBD, which are discussed here.
The Impact of Food Insecurity on Glycemic Control among Individuals with Type 2 Diabetes
Authors: Gordon Barbara
Published: March 2022

The global prevalence of diabetes exceeds half a billion people globally. The Diabetes Prevention Program, a 27-site, randomized clinical trial demonstrated that dietary and other lifestyle changes can prevent more than half (58%) of diabetes cases. Implementation of dietary recommendations can be challenging for those who are not food secure. In a review on the intersection of food insecurity (FI) and diabetes (date range through May 2014), the authors concluded that the lack of access to sufficient, safe, and nutritious food impairs the ability of those with diabetes to implement the dietary modifications required to manage the condition. A challenge to diabetes self-management among this population was adverse social determinants of health. This study assessed insights gleaned about the association between FI and suboptimal glycemic control among adults with type 2 diabetes from research published after May 2014. Conflicting evidence emerged regarding the impact of FI on HbA1c levels among adults with type 2 diabetes. Glycemic control was impacted by social and medical factors. Potential areas for further research are also presented.
The Hallmarks of Glioblastoma: Heterogeneity, Intercellular Crosstalk and Molecular Signature of Invasiveness and Progression
Authors: Torrisi Filippo , Alberghina Cristiana , D’Aprile Simona , Pavone Anna M. , Longhitano Lucia , Giallongo Sebastiano , Tibullo Daniele , Di Rosa Michelino , Zappalà Agata , Cammarata Francesco P. , Russo Giorgio , Ippolito Massimo , Cuttone Giacomo , Li Volti Giovanni , Vicario Nunzio , Parenti Rosalba
Published: March 2022

In 2021 the World Health Organization published the fifth and latest version of the Central Nervous System tumors classification, which incorporates and summarizes a long list of updates from the Consortium to Inform Molecular and Practical Approaches to CNS Tumor Taxonomy work. Among the adult-type diffuse gliomas, glioblastoma represents most primary brain tumors in the neuro-oncology practice of adults. Despite massive efforts in the field of neuro-oncology diagnostics to ensure a proper taxonomy, the identification of glioblastoma-tumor subtypes is not accompanied by personalized therapies, and no improvements in terms of overall survival have been achieved so far, confirming the existence of open and unresolved issues. The aim of this review is to illustrate and elucidate the state of art regarding the foremost biological and molecular mechanisms that guide the beginning and the progression of this cancer, showing the salient features of tumor hallmarks in glioblastoma. Pathophysiology processes are discussed on molecular and cellular levels, highlighting the critical overlaps that are involved into the creation of a complex tumor microenvironment. The description of glioblastoma hallmarks shows how tumoral processes can be linked together, finding their involvement within distinct areas that are engaged for cancer-malignancy establishment and maintenance. The evidence presented provides the promising view that glioblastoma represents interconnected hallmarks that may led to a better understanding of tumor pathophysiology, therefore driving the development of new therapeutic strategies and approaches.
The Biosynthesis and Medicinal Properties of Taraxerol
Authors: Mus Ahmad Asnawi , Goh Lucky Poh Wah , Marbawi Hartinie , Gansau Jualang Azlan
Published: March 2022

Taraxerol is a pentacyclic triterpenoid that is actively produced by some higher plants as part of a defense mechanism. The biosynthesis of taraxerol in plants occurs through the mevalonate pathway in the cytosol, in which dimethylallyl diphosphate (DMAPP) and isopentyl pyrophosphate (IPP) are first produced, followed by squalene. Squalene is the primary precursor for the synthesis of triterpenoids, including taraxerol, β-amyrin, and lupeol, which are catalyzed by taraxerol synthase. Taraxerol has been extensively investigated for its medicinal and pharmacological properties, and various biotechnological approaches have been established to produce this compound using in vitro techniques. This review provides an in-depth summary of the hypothesized taraxerol biosynthetic pathway, the medicinal properties of taraxerol, and recent developments on tissue culture for the in vitro production of taraxerol.
Longitudinal Study of Circulating Biomarkers in Patients with Resectable Pancreatic Ductal Adenocarcinoma
Authors: Dopico Pablo J. , Le Minh-Chau N. , Burgess Benjamin , Yang Zhijie , Zhao Yu , Wang Youxiang , George Thomas J. , Fan Z. Hugh
Published: March 2022

While patients with resectable pancreatic ductal adenocarcinoma (PDAC) show improved survival compared to their non-resectable counterparts, survival remains low owing to occult metastatic disease and treatment resistance. Liquid biopsy based on circulating tumor cells (CTCs) and cell-free DNA (cfDNA) has been shown to predict recurrence and treatment resistance in various types of cancers, but their utility has not been fully demonstrated in resectable PDAC. We have simultaneously tracked three circulating biomarkers, including CTCs, cfDNA, and circulating tumor DNA (ctDNA), over a period of cancer treatment using a microfluidic device and droplet digital PCR (ddPCR). The microfluidic device is based on the combination of filtration and immunoaffinity mechanisms. We have measured CTCs, cfDNA, and ctDNA in a cohort of seven resectable PDAC patients undergoing neoadjuvant therapy followed by surgery, and each patient was followed up to 10 time points over a period of 4 months. CTCs were detectable in all patients (100%) at some point during treatment but were detectable in only three out of six patients (50%) prior to the start of treatment. Median cfDNA concentrations remained comparable to negative controls throughout treatment. ddPCR was able to find KRAS mutations in six of seven patients (86%); however, these mutations were present in only two of seven patients (29%) prior to treatment. Overall, the majority of circulating biomarkers (81% for CTCs and 91% for cfDNA/ctDNA) were detected after the start of neoadjuvant therapy but before surgery. This study suggests that a longitudinal study of circulating biomarkers throughout treatment provides more useful information than those single time-point tests for resectable PDAC patients.
Genetically Modified Soybean Detection Using a Biosensor Electrode with a Self-Assembled Monolayer of Gold Nanoparticles
Authors: Chou Cheng-Chi , Lin Ying-Ting , Kuznetsova Iren , Wang Gou-Jen
Published: March 2022

In this study, we proposed a genosensor that can qualitatively and quantitatively detect genetically modified soybeans using a simple electrode with evenly distributed single layer gold nanoparticles. The DNA sensing electrode is made by sputtering a gold film on the substrate, and then sequentially depositing 1,6-hexanedithiol and gold nanoparticles with sulfur groups on the substrate. Then, the complementary to the CaMV 35S promoter (P35S) was used as the capture probe. The target DNA directly extracted from the genetically modified soybeans rather than the synthesized DNA segments was used to construct the detection standard curve. The experimental results showed that our genosensor could directly detect genetically modified genes extracted from soybeans. We obtained two percentage calibration curves. The calibration curve corresponding to the lower percentage range (1–6%) exhibits a sensitivity of 2.36 Ω/% with R2 = 0.9983, while the calibration curve corresponding to the higher percentage range (6–40%) possesses a sensitivity of 0.1 Ω/% with R2 = 0.9928. The limit of detection would be 1%. The recovery rates for the 4% and 5.7% GMS DNA were measured to be 104.1% and 102.49% with RSD at 6.24% and 2.54%. The gold nanoparticle sensing electrode developed in this research is suitable for qualitative and quantitative detection of genetically modified soybeans and can be further applied to the detection of other genetically modified crops in the future.
Role of the Hydroxyl Groups Coordinated toTiO2 Surface on the Photocatalytic Decomposition of Ethylene at Different Ambient Conditions
Authors: Rychtowski Piotr , Tryba Beata , Skrzypska Agnieszka , Felczak Paula , Sreńscek-Nazzal Joanna , Wróbel Rafał Jan , Nishiguchi Hiroyasu , Toyoda Masahiro
Published: March 2022

The titania pulp—a semi product received from the industrial production of titania white—was submitted for the thermal heating at 400–600 °C under Ar and H2 to obtain TiO2 with different structure and oxygen surface defects. Heating of titania in H2 atmosphere accelerated dehydration and crystallisation of TiO2 compared to heating in Ar. TiO2 prepared at 500 and 600 °C under H2 had some oxygen vacancies and Ti3+ centres (electron traps), whereas TiO2 obtained at 450 °C under H2 exhibited some hole traps centres. The presence of oxygen vacancies induced adsorption of atmospheric CO2. It was evidenced, that ethylene reacted with TiO2 after UV irradiation. Formic acid was identified on TiO2 surface as the reaction product of ethylene oxidation. Hydroxyl radicals were involved in complete mineralisation of ethylene. TiO2 prepared at 500 °C under H2 was poorly active because some active sites for coordination of ethylene molecules were occupied by CO2. The most active samples were TiO2 with high quantity of OH terminal groups. At 50 °C, the physically adsorbed water molecules on titania surface were desorbed, and then photocatalytic decomposition of ethylene was more efficient. TiO2 with high quantity of chemisorbed OH groups was very active for ethylene decomposition. The acidic surface of TiO2 enhances its hydroxylation. Therefore, it is stated that TiO2 having acidic active sites can be an excellent photocatalyst for ethylene decomposition under UV light.
Src-Family Protein Kinase Inhibitors Suppress MYB Activity in a p300-Dependent Manner
Authors: Biyanee Abhiruchi , Yusenko Maria V. , Klempnauer Karl-Heinz
Published: March 2022

Recent studies have disclosed transcription factor MYB as a potential drug target for malignancies that are dependent on deregulated MYB function, including acute myeloid leukemia (AML) and adenoid cystic carcinoma (ACC). Although transcription factors are often regarded as undruggable, successful targeting of MYB by low-molecular-weight compounds has recently been demonstrated. In an attempt to repurpose known drugs as novel MYB-inhibitory agents, we have screened libraries of approved drugs and drug-like compounds for molecules with MYB-inhibitory potential. Here, we present initial evidence for the MYB-inhibitory activity of the protein kinase inhibitors bosutinib, PD180970 and PD161570, that we identified in a recent screen. We show that these compounds interfere with the activity of the MYB transactivation domain, apparently by disturbing the ability of MYB to cooperate with the coactivator p300. We show that treatment of the AML cell line HL60 with these compounds triggers the up-regulation of the myeloid differentiation marker CD11b and induces cell death. Importantly, we show that these effects are significantly dampened by forced expression of an activated version of MYB, confirming that the ability to suppress MYB function is a relevant activity of these compounds. Overall, our work identifies several protein kinase inhibitors as novel MYB-inhibitory agents and suggests that the inhibition of MYB function may play a role in their pharmacological impact on leukemic cells.
Inflammatory Caspase Activity Mediates HMGB1 Release and Differentiation in Myoblasts Affected by Peripheral Arterial Disease
Authors: Ferrari Ricardo , Xie Bowen , Assaf Edwyn , Morder Kristin , Scott Melanie , Liao Hong , Calderon Michael J. , Ross Mark , Loughran Patricia , Watkins Simon C. , Pipinos Iraklis , Casale George , Tzeng Edith , McEnaney Ryan , Sachdev Ulka
Published: March 2022

Introduction: We previously showed that caspase-1 and -11, which are activated by inflammasomes, mediate recovery from muscle ischemia in mice. We hypothesized that similar to murine models, inflammatory caspases modulate myogenicity and inflammation in ischemic muscle disease. Methods: Caspase activity was measured in ischemic and perfused human myoblasts in response to the NLRP3 and AIM2 inflammasome agonists (nigericin and poly(dA:dT), respectively) with and without specific caspase-1 or pan-caspase inhibition. mRNA levels of myogenic markers and caspase-1 were assessed, and protein levels of caspases-1, -4, -5, and -3 were measured by Western blot. Results: When compared to perfused cells, ischemic myoblasts demonstrated attenuated MyoD and myogenin and elevated caspase-1 mRNA. Ischemic myoblasts also had significantly higher enzymatic caspase activity with poly(dA:dT) (p < 0.001), but not nigericin stimulation. Inhibition of caspase activity including caspase-4/-5, but not caspase-1, blocked activation effects of poly(dA:dT). Ischemic myoblasts had elevated cleaved caspase-5. Inhibition of caspase activity deterred differentiation in ischemic but not perfused myoblasts and reduced the release of HMGB1 from both groups. Conclusion: Inflammatory caspases can be activated in ischemic myoblasts by AIM2 and influence ischemic myoblast differentiation and release of pro-angiogenic HMGB1. AIM2 inflammasome involvement suggests a role as a DNA damage sensor, and our data suggest that caspase-5 rather than caspase-1 may mediate the downstream mediator of this pathway.
Alterations in Protein Translation and Carboxylic Acid Catabolic Processes in Diabetic Kidney Disease
Authors: Collins Kimberly S. , Eadon Michael T. , Cheng Ying-Hua , Barwinska Daria , Melo Ferreira Ricardo , McCarthy Thomas W. , Janosevic Danielle , Syed Farooq , Maier Bernhard , El-Achkar Tarek M. , Kelly Katherine J. , Phillips Carrie L. , Hato Takashi , Sutton Timothy A. , Dagher Pierre C.
Published: March 2022

Diabetic kidney disease (DKD) remains the leading cause of end-stage kidney disease despite decades of study. Alterations in the glomerulus and kidney tubules both contribute to the pathogenesis of DKD although the majority of investigative efforts have focused on the glomerulus. We sought to examine the differential expression signature of human DKD in the glomerulus and proximal tubule and corroborate our findings in the db/db mouse model of diabetes. A transcriptogram network analysis of RNAseq data from laser microdissected (LMD) human glomerulus and proximal tubule of DKD and reference nephrectomy samples revealed enriched pathways including rhodopsin-like receptors, olfactory signaling, and ribosome (protein translation) in the proximal tubule of human DKD biopsy samples. The translation pathway was also enriched in the glomerulus. Increased translation in diabetic kidneys was validated using polyribosomal profiling in the db/db mouse model of diabetes. Using single nuclear RNA sequencing (snRNAseq) of kidneys from db/db mice, we prioritized additional pathways identified in human DKD. The top overlapping pathway identified in the murine snRNAseq proximal tubule clusters and the human LMD proximal tubule compartment was carboxylic acid catabolism. Using ultra-performance liquid chromatography–mass spectrometry, the fatty acid catabolism pathway was also found to be dysregulated in the db/db mouse model. The Acetyl-CoA metabolite was down-regulated in db/db mice, aligning with the human differential expression of the genes ACOX1 and ACACB. In summary, our findings demonstrate that proximal tubular alterations in protein translation and carboxylic acid catabolism are key features in both human and murine DKD.
A Sweet Cherry Glutathione S-Transferase Gene, PavGST1, Plays a Central Role in Fruit Skin Coloration
Authors: Qi Xiliang , Liu Congli , Song Lulu , Dong Yuanxin , Chen Lei , Li Ming
Published: March 2022

Sweet cherry, an economically important horticultural crop, has strong antioxidant activity. The fruits contain compounds potentially beneficial to human health—particularly anthocyanins, which are synthesized in cytosol and predominantly accumulated in vacuoles. Although anthocyanin levels differ among dark-red, blush, and yellow sweet cherry cultivars, the regulatory mechanism of anthocyanin transport and accumulation is not well understood in this species. In this study, we identified 53 glutathione S-transferase genes (PavGSTs) from sweet cherry and found that PavGST1 expression was well correlated with anthocyanin accumulation in cultivars with different fruit skin colors. TRV-mediated virus-induced silencing of PavGST1 decreased anthocyanin accumulation in sweet cherry fruits and downregulated the expressions of anthocyanin biosynthetic and regulatory genes. In addition, transient overexpression of PavGST1 promoted anthocyanin accumulation. Furthermore, yeast one-hybrid and dual-luciferase assays revealed that PavMYB10.1 and PavMYB75 directly bind to different MYB binding sites of the PavGST1 promoter (MBS-1 and MBS-3) to activate PavGST1 transcription. According to our results, PavGST1 plays a central role in sweet cherry fruit anthocyanin accumulation. Our findings provide novel insights into the coordinative regulatory mechanisms of PavGST1 and PavMYBs in anthocyanin accumulation in sweet cherry.
A Special Issue in Honor of Professor Josef Michl
Authors: Alabugin Igor , Klán Petr
Published: March 2022

This Special Issue of Chemistry is dedicated to Professor Josef Michl [...]
Early Intervention in Families with Preterm Infants: A Review of Findings from a Randomized Controlled Trial Following Children Up to 9 Years of Age
Authors: Ulvund Stein Erik
Published: March 2022

The Tromsø Intervention Study on Preterms (TISP) randomized 146 preterm born children (<2000 g) either to the Mother-Infant Transaction Program-Modified (MITP-M, n = 72) or to a preterm control group (n = 74). In addition, 75 full-term babies were followed up until 9 years of age. TISP was conducted at the University Hospital Northern Norway (UNN) and only infants who did not have congenital anomalies and families where the mothers’ native language was Norwegian were included. The study investigates the effect of MITP-M on cognitive and social development including behavioral problems, quality of life and stress in the family. The results have so far been published in various journals. The aim of this article is to give a comprehensive overall presentation of the main findings and discuss implications for clinical practice and further research. Parents in the intervention group were superior in “reading” their infants’ temperament, and at 3, 5 and 7 years of age the intervention group scored significantly higher on well-known tests of cognitive outcome. At 9 years of age, the intervention group had fewer attentional problems, better school achievements and a better quality of life. From the first year onwards, mothers and fathers in the intervention group reported lower levels of stress than parents of in the preterm control group.
The Impact of Parental Alienating Behaviours on the Mental Health of Adults Alienated in Childhood
Authors: Verhaar Suzanne , Matthewson Mandy Louise , Bentley Caitlin
Published: March 2022

This study qualitatively investigated the mental health of adults exposed to parental alienating behaviours in childhood. Research suggests that exposure to parental alienating behaviours in childhood can have a profound impact on the mental health of those children later in life, including experiencing anxiety disorders and trauma reactions. An international sample of 20 adults exposed to parental alienating behaviours in childhood participated in semi-structured interviews on their experience and its impact. Four themes were identified: mental health difficulties, including anxiety disorders and trauma reactions, emotional pain, addiction and substance use, and coping and resilience. Intergenerational transmission of parental alienation was found. Confusion in understanding their experience of alienation, the mental health sequelae, and elevated levels of suicidal ideation were found. This study demonstrated the insidious nature of parental alienation and parental alienating behaviours and provided further evidence of these behaviours as a form of emotional abuse.
Lung Function Tests, Quality of Life and Telemedicine: Three Windows on the Multifaceted World of Asthma in Adolescents
Authors: Nucera Eleonora , Rizzi Angela , Agrosì Chiara , Lohmeyer Franziska Michaela , Inchingolo Riccardo
Published: March 2022

Asthma is a heterogeneous disease usually characterized by chronic airway inflammation and recognized as the most prevalent chronic illness among children. Despite this, the knowledge as to how asthma affects adolescents is still scarce. One of the main management problems of asthmatic adolescents is the poor adherence to pharmacological and non-pharmacological treatments. The assessment of respiratory function and the impact on quality of life are still two crucial challenges in the management of asthmatic adolescents. Additionally, the COVID-19 pandemic has prompted physicians to explore complementary management strategies including telemedicine technologies. This review aims to provide an update on the contribution of respiratory functional tests, how asthma affects quality of life of adolescents and, finally, how telemedicine contributes to the management of adolescent asthmatics during the COVID-19 pandemic.
Ulcerative Colitis Seems to Imply Oral Microbiome Dysbiosis
Authors: Molinero Natalia , Taladrid Diego , Zorraquín-Peña Irene , de Celis Miguel , Belda Ignacio , Mira Alex , Bartolomé Begoña , Moreno-Arribas M. Victoria
Published: March 2022

Ulcerative colitis (UC) is a recurrent pathology of complex etiology that has been occasionally associated with oral lesions, but the overall composition of the oral microbiome in UC patients and its role in the pathogenesis of the disease are still poorly understood. In this study, the oral microbiome of UC patients and healthy individuals was compared to ascertain the possible changes in the oral microbial communities associated with UC. For this, the salivary microbiota of 10 patients diagnosed with an active phase of UC and 11 healthy controls was analyzed by 16S rRNA gene sequencing (trial ref. ISRCTN39987). Metataxonomic analysis revealed a decrease in the alpha diversity and an imbalance in the relative proportions of some key members of the oral core microbiome in UC patients. Additionally, Staphylococcus members and four differential species or phylotypes were only present in UC patients, not being detected in healthy subjects. This study provides a global snapshot of the existence of oral dysbiosis associated with UC, and the possible presence of potential oral biomarkers.
Triazine 2D Nanosheets as a New Class of Nanomaterials: Crystallinity, Properties and Applications
Authors: Zakharov Valery N. , Kudryavtsev Igor K. , Dunaev Sergey F. , Paseshnichenko Kseniya A. , Aslanov Leonid A.
Published: March 2022

Based on the recent (2015–2021) literature data, the authors analyze the mutual dependence of crystallinity/amorphism and specific surface area and porosity in covalent triazine frameworks (CTFs), taking into account thermodynamic and kinetic control in the synthesis of these 2D nanosheets. CTFs have now become a promising new class of high-performance porous organic materials. They can be recycled and reused easily, and thus have great potential as sustainable materials. For 2D CTFs, numerous examples are given to support the known rule that the structure and properties of any material with a given composition depend on the conditions of its synthesis. The review may be useful for elder students, postgraduate students, engineers and research fellows dealing with chemical synthesis and modern nanotechnologies based on 2D covalent triazine frameworks.
Shape Optimization of a Shell in Comsol Multiphysics
Authors: Ermakova Evgenia , Elberdov Timur , Rynkovskaya Marina
Published: March 2022

Optimization calculations are currently an actual and in-demand direction of computer-aided design. It allows not only the identification of the future characteristics of an object, but also the implementation of its exact model using a set of various optimization algorithms. The advent of digital modeling has significantly facilitated the approach to optimization and its methods. Many software systems are equipped with capabilities not only for calculating the design, but also for finding its optimal variant. The calculation programs can include a special optimization module that can be based on one or more mathematical methods. The purpose of the present study is to explore a process of shape optimization through the calculation of two shells: the simple one (spherical dome) and complex one (helicoid) in Comsol Multiphysics using three optimization methods: MMA, SNOPT and IPOPT. Additionally, special attention is paid to the construction of a mesh for calculations and two types of selected element sizes: finer and fine. Then, the important task is to compare the obtained results and to find the most optimal method and most effective design solution for each shell. When calculating the sphere, the most suitable solution was obtained using the IPOPT method, with the help of which it was possible to achieve an optimal reduction in the dome along the z-axis. When calculating the helicoid, all methods showed approximately the same values and equally changed the angle of inclination of the surface relative to the horizontal plane.
Microstructure and High-Temperature Properties of TC31 Alloy Manufactured by Laser Melting Deposition
Authors: Li Hepeng , Guo Bin , Zong Yingying , Shan Debin
Published: March 2022

This paper presents a comprehensive study conducted to optimize the mechanical properties for a laser-melting-deposition fabricated TC31 (Ti-Al-Sn-Zr-Mo-Nb-W-Si) alloy, which is a newly developed high-temperature alloy used in the aerospace industry. The results showed that the laser melting deposition (LMD)-built sample exhibited columnar structures with very fine α-laths inside. Annealing and solution treatment resulted in an α+β lamellar structure consisting of α-laths and β-films, of which thicknesses depended on the temperature. Solution treatment and subsequent aging did not significantly change the lamellar structure. However, aging at 650 °C led to the formation of nanoscale α precipitates within the remaining β, while aging at 750 °C resulted in coarse α precipitates. The solution-treated samples exhibited the best combination of strength and ductility at room temperature, ultimate tensile strength of 1047 MPa, and elongation of 13.0%, which is superior to the wrought TC31 counterparts. The sample after solution treatment at 980 °C and subsequent aging at 650 °C obtained an attractive combination of strength and ductility both at room temperature and high temperature due to the synergistic effect of the soft α + β lamellar structure and hard fine α precipitates. These findings provide valuable information on developments of LMD-built TC31 alloy for aerospace applications and shed light on AM of other titanium alloys with desirable high-temperature properties.
Role of Oxygen and Fluorine in Passivation of the GaSb(111) Surface Depending on Its Termination
Authors: Bakulin Alexander V. , Chumakova Lora S. , Korchuganov Aleksandr V. , Kulkova Svetlana E.
Published: March 2022

The mechanism of the chemical bonding of oxygen and fluorine on the GaSb(111) surface depending on its termination is studied by the projector augmented-waves method within density functional theory. It is shown that on an unreconstructed (111) surface with a cation termination, the adsorption of fluorine leads to the removal of surface states from the band gap. The binding energy of fluorine on the cation-terminated surface in the most preferable Ga-T position is lower by ~0.4 eV than that of oxygen, but it is significantly lower (by ~0.8 eV) on the anion-terminated surface. We demonstrate that the mechanism of chemical bonding of electronegative adsorbates with the surface has an ionic–covalent character. The covalence of the O–Sb bond is higher than the F–Sb one, and it is higher than both O–Ga and F–Ga bonds. Trends in the change in the electronic structure of the GaSb(111) surface upon adsorption of fluorine and oxygen are discussed. It is found that an increase in the oxygen concentration on the Sb-terminated GaSb(111) surface promotes a decrease in the density of surface states in the band gap.
Genotyping-by-Sequencing Analysis Shows That Siberian Lindens Are Nested within Tilia cordata Mill
Authors: Shekhovtsov Sergei V. , Shekhovtsova Irina N. , Kosterin Oleg E.
Published: March 2022

Tilia sibirica and T. nasczokinii are considered to be endemic Siberian linden species. They have very small distributions located hundreds to thousands of kilometers away from other lindens. It is unclear how closely these species are related to the widespread Tilia cordata: according to the current hypotheses, they could be pre-Pleistocene relicts or remnants of the recent continuous range of T. cordata that existed during the Holocene climatic optimum. Earlier studies detected significant differences between T. sibirica, T. nasczokinii, and T. cordata in microsatellite loci, but not in plastid sequences. Here we performed a phylogenetic analysis of several linden species based on GBS data. The obtained GBS sequences were assembled to create phylogenetic trees based on 16,000–294,000 variable sites. We found that T. cordata and the two putative Siberian species formed a monophyletic group. It consisted of three clades: the basal clade containing specimens from the Caucasus, and two sister clades representing populations from the East European Plains+the Urals and Siberia, respectively. Neither of the Siberian species was related to the Far Eastern T. amurensis, as was hypothesized earlier. Our study suggests that the colonization of Europe and Siberia after the Last Glacial Maximum occurred from different glacial refugia.
Noninvasive Evaluation of Myocardial Work in Patients with Chronic Kidney Disease Using Left Ventricular Pressure-Strain Loop Analysis
Authors: Liu Xiaohua , Chen Lixin , Zhong Xiaofang , Peng Guijuan , Sheng Yuanyuan , Li Jian , Liu Qian , Shi Bobo , Huang Yuxiang , Xu Jinfeng , Liu Yingying
Published: March 2022

(1) Objective: To evaluate myocardial injury by observing the different parameters of global myocardial work (MW) by left ventricular pressure-strain loop (PSL) analysis in patients with chronic kidney disease (CKD). (2) Methods: According to the left ventricular mass index, the study patients with CKD were further divided into two groups: the left ventricular normal group (CKDN-LVH, 59) and left ventricular hypertrophy group (CKDLVH, 46). Thirty-three healthy controls (CON) matched in age and sex with the CKD group were recruited. The routine ultrasonic parameters were obtained by routine TTE, and the strain index and different parameters of the left ventricular MW were obtained by dynamic image offline analysis. (3) Results: This study found that (1) compared with the CON group, the CKDN-LVH group had a significantly increased global waste work (GWW) and significantly decreased global work efficiency (GWE), the GWW further increased, and GWE further decreased in the CKDLVH group. There was no significant change in the global work index (GWI) and global constructive work index (GCW) in the CKDN-LVH group, but the GWI and GCW in the CKDLVH group were significantly increased. (2) According to the grouping analysis of systolic blood pressure (SBP), we found that the GWW increased and GWE decreased in CKD patients with an elevated SBP. (3) Correlation analysis showed that the increase of the peak strain dispersion, SBP, and left ventricular mass index and the decrease of the estimated glomerular filtration rate were significantly correlated with the decrease of the GWE and the increase of the GWW. (4) Receiver operating characteristic curve analysis showed that the area under the curve (AUC) of myocardial damage induced by the GWE and GWW in the CKD group and CON group was higher than that of left ventricular global longitudinal strain (AUCs: 0.87 and 0.878 versus 0.72, respectively). (4) Conclusions: Noninvasive left ventricular PSL analysis can be used to evaluate the global MW in patients with CKD. The study justified the role of GWW in the noninvasive assessment of myocardial function in patients with CKD.
Systemic Inflammatory Predictors of In-Hospital Mortality in COVID-19 Patients: A Retrospective Study
Authors: Kudlinski Bartosz , Zgoła Dominika , Stolińska Marta , Murkos Magdalena , Kania Jagoda , Nowak Pawel , Noga Anna , Wojciech Magdalena , Zaborniak Gabriel , Zembron-Lacny Agnieszka
Published: March 2022

The purpose of this study was to investigate whether routine blood tests and clinical characteristics can predict in-hospital mortality in COVID-19. Clinical data of 285 patients aged 59.7 ± 10.3 yrs. (males n = 189, females n = 96) were retrospectively collected from December 2020 to June 2021. Routine blood tests were recorded within the 1st hour of admission to hospital. The inflammatory variables, such as C-reactive protein (CRP), procalcitonin (PCT), neutrophils–lymphocyte ratio (NLR) and the systemic inflammatory index (SII), exceeded the reference values in all patients and were significantly higher in deceased patients (n = 108) compared to survivors (n = 177). The log-rank test for comparing two survival curves showed that patients aged ≥60.5 years, with PCT ≥ 0.188 ng/mL or NLR ≥ 11.57 103/µL were at a greater risk of death. NLR demonstrated a high impact on the COVID-19 mortality (HR 1.317; 95%CI 1.004–1.728; p < 0.05), whereas CRP and SII showed no effect (HR 1.000; 95%CI 1.000–1.004; p = 0.085 and HR 1.078; 95%CI 0.865–1.344; p = 0.503, respectively). In the first Polish study including COVID-19 patients, we demonstrated that age in relation to simple parameters derived from complete blood cell count has prognostic implications in the course of COVID-19 and can identify the patients at a higher risk of in-hospital mortality.
Factors Influencing Mammographic Density in Asian Women: A Retrospective Cohort Study in the Northeast Region of Peninsular Malaysia
Authors: Hanis Tengku Muhammad , Arifin Wan Nor , Haron Juhara , Wan Abdul Rahman Wan Faiziah , Ruhaiyem Nur Intan Raihana , Abdullah Rosni , Musa Kamarul Imran
Published: March 2022

Mammographic density is a significant risk factor for breast cancer. In this study, we identified the risk factors of mammographic density in Asian women and quantified the impact of breast density on the severity of breast cancer. We collected data from Hospital Universiti Sains Malaysia, a research- and university-based hospital located in Kelantan, Malaysia. Multivariable logistic regression was performed to analyse the data. Five significant factors were found to be associated with mammographic density: age (OR: 0.94; 95% CI: 0.92, 0.96), number of children (OR: 0.88; 95% CI: 0.81, 0.96), body mass index (OR: 0.88; 95% CI: 0.85, 0.92), menopause status (yes vs. no, OR: 0.59; 95% CI: 0.42, 0.82), and BI-RADS classification (2 vs. 1, OR: 1.87; 95% CI: 1.22, 2.84; 3 vs. 1, OR: 3.25; 95% CI: 1.86, 5.66; 4 vs. 1, OR: 3.75; 95% CI: 1.88, 7.46; 5 vs. 1, OR: 2.46; 95% CI: 1.21, 5.02; 6 vs. 1, OR: 2.50; 95% CI: 0.65, 9.56). Similarly, the average predicted probabilities were higher among BI-RADS 3 and 4 classified women. Understanding mammographic density and its influencing factors aids in accurately assessing and screening dense breast women.
An Information-Theoretic Analysis of the Cost of Decentralization for Learning and Inference under Privacy Constraints
Authors: Jose Sharu Theresa , Simeone Osvaldo
Published: March 2022

In vertical federated learning (FL), the features of a data sample are distributed across multiple agents. As such, inter-agent collaboration can be beneficial not only during the learning phase, as is the case for standard horizontal FL, but also during the inference phase. A fundamental theoretical question in this setting is how to quantify the cost, or performance loss, of decentralization for learning and/or inference. In this paper, we study general supervised learning problems with any number of agents, and provide a novel information-theoretic quantification of the cost of decentralization in the presence of privacy constraints on inter-agent communication within a Bayesian framework. The cost of decentralization for learning and/or inference is shown to be quantified in terms of conditional mutual information terms involving features and label variables.
Unconditional Authentication Based on Physical Layer Offered Chain Key in Wireless Communication
Authors: Wang Shaoyu , Huang Kaizhi , Xu Xiaoming , Hu Xiaoyan , Yang Jing , Jin Liang
Published: March 2022

Authentication is a critical issue in wireless communication due to the impersonation and substitution attacks from the vulnerable air interface launched by the malicious node. There are currently two kinds of authentication research in wireless communication. One is based on cryptography and relies on computational complexity, the other is based on physical layer fingerprint and can not protect data integrity well. Both of these approaches will become insecure when facing attackers with infinite computing power. In this paper, we develop a wireless unconditional authentication framework based on one-time keys generated from wireless channel. The proposed unconditional authentication framework provides a new perspective to resist infinite computing power attackers. We study the performance of the unconditional authentication framework in this paper. First, a physical layer offered chain key (PHYLOCK) structure is proposed, which can provide one-time keys for unconditional authentication. The physical layer offered chain keys are generated by XORing the physical layer updated keys extracted from the current channel state information (CSI) and the previous chain keys. The security of PHYLOCK is analyzed from the perspective of information theory. Then, the boundary of the deception probability is conducted. It is shown that unconditional authentication can achieve a probability of deception 2−12Hk, where Hk is the entropy of the one-time key used for one message. Finally, the conditions for unconditional authentication are listed. Our analysis shows that the length of the key and the authentication code need to be twice the length of the message and the encoding rules of the authentication code need to satisfy the restrictions we listed.
Enhancing AES Using Chaos and Logistic Map-Based Key Generation Technique for Securing IoT-Based Smart Home
Authors: Rahman Ziaur , Yi Xun , Billah Mustain , Sumi Mousumi , Anwar Adnan
Published: March 2022

The Internet of Things (IoT) has brought new ways for humans and machines to communicate with each other over the internet. Though sensor-driven devices have largely eased our everyday lives, most IoT infrastructures have been suffering from security challenges. Since the emergence of IoT, lightweight block ciphers have been a better option for intelligent and sensor-based applications. When public-key infrastructure dominates worldwide, the symmetric key encipherment such as Advanced Encryption Standard (AES) shows immense prospects to sit with the smart home IoT appliances. As investigated, chaos motivated logistic map shows enormous potential to secure IoT aligned real-time data communication. The unpredictability and randomness features of the logistic map in sync with chaos-based scheduling techniques can pave the way to build a particular dynamic key propagation technique for data confidentiality, availability and integrity. After being motivated by the security prospects of AES and chaos cryptography, the paper illustrates a key scheduling technique using a 3-dimensional S-box (substitution-box). The logistic map algorithm has been incorporated to enhance security. The proposed approach has applicability for lightweight IoT devices such as smart home appliances. The work determines how seeming chaos accelerates the desired key-initiation before message transmission. The proposed model is evaluated based on the key generation delay required for the smart-home sensor devices.
Implementation of Soft Decoding Mechanism for Addressing Nonlinearities in Long-Distance Optical Transmission System
Authors: Armghan Ammar
Published: March 2022

A soft decoding technique is discussed in this paper to improve the performance of long-distance optical networks (LDOTNs). LDOTNs are affected by phase noise and nonlinearities generated inside the fiber. The investigations of the proposed LDOTN were carried out by dual-polarization 16-quadrature amplitude modulation (DP-16QAM), DP-64QAM over single-mode fiber (SMF) and digital signal processing (DSP) methodologies. The improved performance of the presented mechanism is discussed over SMF based on constellation shaping (CS). The CS of the presented LDOTN is then compared to the standard 16-QAM and 64-QAM using international telecommunication union-telecommunication (ITU-T) standard G-652.D and G-657.A1 SMF. The soft detecting procedure enables the LDOTNs to attain significant outcomes.
Fully Automatic Analysis of Muscle B-Mode Ultrasound Images Based on the Deep Residual Shrinkage U-Net
Authors: Zheng Weimin , Zhou Linxueying , Chai Qingwei , Xu Jianguo , Liu Shangkun
Published: March 2022

The parameters of muscle ultrasound images reflect the function and state of muscles. They are of great significance to the diagnosis of muscle diseases. Because manual labeling is time-consuming and laborious, the automatic labeling of muscle ultrasound image parameters has become a research topic. In recent years, there have been many methods that apply image processing and deep learning to automatically analyze muscle ultrasound images. However, these methods have limitations, such as being non-automatic, not applicable to images with complex noise, and only being able to measure a single parameter. This paper proposes a fully automatic muscle ultrasound image analysis method based on image segmentation to solve these problems. This method is based on the Deep Residual Shrinkage U-Net(RS-Unet) to accurately segment ultrasound images. Compared with the existing methods, the accuracy of our method shows a great improvement. The mean differences of pennation angle, fascicle length and muscle thickness are about 0.09°, 0.4 mm and 0.63 mm, respectively. Experimental results show that the proposed method realizes the accurate measurement of muscle parameters and exhibits stability and robustness.
Run-Time Hierarchical Management of Mapping, Per-Cluster DVFS and Per-Core DPM for Energy Optimization
Authors: Qiu Weiming , Chen Yonghao , Chen Dihu , Su Tao , Yang Simei
Published: March 2022

Heterogeneous cluster-based multi/many-core systems (e.g., ARM big.LITTLE, supporting dynamic voltage and frequency scaling (DVFS) at cluster level and dynamic power management (DPM) at core level) have attracted much attention to optimize energy on modern embedded systems. For concurrently executing applications on such a platform, this paper aims to study how to appropriately apply the three system configurations (mapping, DVFS, and DPM) to reduce both dynamic and static energy. To this end, this paper first formulates the dependence of the three system configurations on heterogeneous cluster-based systems as a 0–1 integrated linear programming (ILP) model, taking into account run-time configuration overheads (e.g., costs of DPM mode switching and task migration). Then, with the 0–1 ILP model, different run-time strategies (e.g., considering the three configurations in fully separate, partially separate, and holistic manners) are compared based on a hierarchical management structure and design-time prepared data. Experimental case studies offer insights into the effectiveness of different management strategies on different platform sizes (e.g., #cluster × #core, 2 × 4, 2 × 8, 4 × 4, 4 × 8), in terms of application migration, energy efficiency, resource efficiency, and complexity.