bluuebunny/crossref_metadata_2025 huggingface dataset:
Quality of Life after Cataract Surgery
Authors: Błachnio Klaudia , Dusińska Aleksandra , Szymonik Julia , Juzwiszyn Jan , Bestecka Monika , Chabowski Mariusz
Published: September 2024

Background: The impact of medical intervention on a patient’s quality of life (QoL) is more and more important. Treatment success is defined not only in terms of the success of the procedure performed but also with regard to its impact on different areas of the patient’s life. The aim of the study was to assess the QoL of patients after cataract surgery and identify factors that affect it. Methods: Between January and March 2018, a survey was carried out among 100 patients who had undergone cataract surgery with intraocular lens implantation at the ‘Spektrum’ Clinical Ophthalmology Centre in Wrocław. The World Health Organization Quality of Life—BREF (WHOQOL-BREF) questionnaire and Illness Acceptance Scale (AIS) were used. Results: Most respondents (67%) rated their overall health as very good. The median score on the AIS was 34 (31.5–39), indicating a high level of illness acceptance. There was no statistically significant relationship (p > 0.05) between sex and QoL nor between the level of illness acceptance and QoL. We found no statistically significant relationships between place of residence and QoL (p > 0.05) nor between place of residence and AIS. Conclusions: The respondents reported the highest QoL scores for the environment domain and the lowest QoL scores for the social relationships domain. QoL had a positive impact on illness acceptance among the study patients. Younger patients (aged 50 or under) reported significantly higher scores for all the domains of QoL. Being employed was found to be associated with better QoL and greater illness acceptance.
Novel Chitosan-Gelatin Scaffold with Valproic Acid Augments In Vitro Osteoblast Differentiation of Mesenchymal Stem Cells
Authors: Alghofaily Maha , Alsalleeh Fahd , Alssum Lamees , Muthurangan Manikandan , Alfayez Musaad , Weir Michael D. , Xu Hockin H. K.
Published: September 2024

The study aimed to develop a biodegradable scaffold incorporating valproic acid (VPA) for improved human bone marrow-derived mesenchymal stem cell (hBMSC) proliferation, differentiation, and bone mineral synthesis. A chitosan–gelatin (CH-G) scaffold was fabricated and loaded with varying concentrations of VPA (1, 3, 5 mM/L). In vitro studies assessed drug release, cell proliferation, morphology, mineralization, and gene expression. VPA was rapidly released from the scaffold, with over 90% cumulative release within seven days. Cells cultured on VPA-loaded scaffolds exhibited significantly enhanced proliferation and mineralization compared to the control. VPA treatment upregulated osteocalcin and runt-related transcription factor 2 (Runx-2) expression, key markers of osteogenic differentiation. The CH-G scaffold, particularly with 1 mM/L VPA, demonstrates excellent biocompatibility and promotes hBMSC-mediated bone regeneration. This novel approach holds promise for future applications in bone tissue engineering.
Galling-Free Forging of Titanium Using Carbon-Supersaturated SiC Coating Dies
Authors: Aizawa Tatsuhiko , Fukuda Tatsuya
Published: September 2024

The thermal chemical vapor deposition (CVD) process was utilized to fabricate 6H-structured SiC coating dies with carbon control. The carbon-rich clusters along the SiC grain boundaries acted as a pinning site to suppress irregular crystal growth and to homogenize the fine-grained structure. These massive carbon-supersaturated (MCSed) SiC dies with a thickness of 4 mm were utilized for upsetting pure titanium bars in dry and cold conditions. Under a stress gradient from the contact interface to the depth of the SiC coating, the carbon solute isolated from these carbon clusters diffused through the grain boundaries and formed free carbon agglomerates on the contact interface to the pure titanium bars. These in situ-formed free carbon agglomerates acted as a solid lubricant to sustain the friction coefficient at 0.09 at the hot spots on the contact interface and to protect the dies and bars from severe adhesive wearing.
Development of a Novel Adsorbent Derived from Olive Mill Solid Wastes for Enhanced Removal of Methylene Blue
Authors: Hamieh Malak , Tabaja Nabil , Tlais Sami , Koubaissy Bachar , Hammoud Mohammad , Chawraba Khaled , Hamieh Tayssir , Toufaily Joumana
Published: September 2024

Industrial wastewater discharge, tainted with hazardous substances, including dyes like methylene blue (MB) from the textile sector, further emphasizes the need for water treatment to produce safe drinking water. This study explores the potential of olive mill solid waste, an abundant and cost-effective agricultural waste in Mediterranean regions, to yield high-quality activated carbon (AC) with zinc chloride activation for MB adsorption. The activation process, carried out at a modest temperature of 500 °C without the need for an inert atmosphere, resulted in AC with remarkable characteristics, boasting a substantial surface area of 1184 cm2·g−1 and a total pore volume of 0.824 cm3·g−1. Extensive characterization of the AC was carried out through a large range of surface techniques. The pH of the solution had minimal influence on MB adsorption, the maximum removal was 95%, which was under slightly acidic pH conditions (5.8), and the adsorbent dose was 0.4 g·L−1 for a 50 mg·L−1 MB concentration. Equilibrium data pertaining to MB adsorption were subjected to fitting with different models, namely Langmuir, Freundlich, and Temkin. Notably, the Langmuir model exhibited the best fit, revealing a maximum monolayer adsorption capacity of 500 mg·g−1 at 25 °C, and the adsorption kinetics closely followed a pseudo-second-order model.
Effect of Deposition Parameters on Micromechanical Properties and Machining Performance of CrN Coating for Wet Finish Turning of Ti6Al4V Alloy
Authors: Chowdhury Mohammad Shariful Islam , Bose Bipasha , Akter Shahana , Veldhuis Stephen Clarence
Published: September 2024

This study aims to optimize the performance of CrN coatings deposited on WC cutting tools for machining Ti6Al4V alloy, where the formation of built-up edge (BUE) is a prevalent and critical issue. In-house CrN coatings were developed using the PVD (Physical Vapor Deposition) process, with variations in deposition parameters including nitrogen gas pressure, bias voltage, and coating thickness. A comprehensive experimental approach encompassing deposition, characterization, and machining performance evaluation was employed to identify the optimal deposition conditions. The results indicated that CrN coatings deposited at a nitrogen gas pressure of 4 Pa, a bias voltage of −50 V, and a thickness of 1.81 µm exhibited superior performance, significantly reducing BUE formation and tool wear. These optimized coatings demonstrated enhanced properties, such as a higher elastic modulus and a lower coefficient of friction, which contributed to improved tool life and machining performance. Comparative studies with commercial CrN coatings revealed that the in-house developed coatings outperformed the commercial variants by approximately 65% in tool life, owing to their superior mechanical properties and reduced friction. This research highlights the potential of tailored CrN coatings for advanced machining applications and emphasizes the importance of optimizing deposition parameters to achieve high-performance tool coatings.
Corncob-Derived Activated Carbon as Electrode Material for High-Performance Supercapacitor
Authors: Dong Lili , Pan Chenghao , Ji Yongfeng , Ren Suxia , Lei Tingzhou
Published: September 2024

In this study, corncob was explored as a low-cost and abundant precursor for the preparation of activated carbon via carbonization and the KOH activation method. The alkaline/biochar ratios varied from 3:1 to 5:1, and the activation temperatures ranged from 700 to 900 °C. The characterized results reveal that the alkaline/biochar ratios and activation temperatures had a remarkable influence on the morphology and microstructure of as-prepared activated carbon (CACT-R). The CACT-R presented a porous structure with a large number of micropores and a small number of mesopores. The reasonable distribution of micropores and mesopores endows the ideal structure for ion transfer and charge storage. The optimal sample CAC700-4 exhibited the best capacitive performance with a specific capacitance of 260 F/g at 1 A/g. Moreover, the assembled CAC700-4//CAC700-4 symmetric supercapacitor showed a high energy density of 14.3 Wh/kg at a power density of 250 W/kg in 6 M KOH electrolyte. It also has a capacitance retention of 95.5% after 10,000 cycles, indicating its excellent cycle stability. These results indicate that corncob-derived activated carbon provides the possible application of biomass waste in high-performance supercapacitors.
The Various Radii Circle Packing Problem in a Triangle
Authors: Chiang Ching-Shoei , Chiang Yi-Ting
Published: September 2024

Malfatti’s problem is the problem of fitting three circles into a triangle such that they are tangent to each other and each circle is also tangent to a pair of the triangle’s sides. This problem has been extended to include Tn = 1 + 2 + … + n circles inside the triangle with special tangency properties among the circles and triangle sides; this problem is referred to as the extended Malfatti problem or the Tri(Tn) problem. In the extended Malfatti problem, the number of circles in the triangle is a triangle number because the tangency properties between the internal circles and the three sides of the triangle have a special type of structure; that is, the corner circle is tangent to two sides of the triangle and two other circles, the boundary circles are tangent to one side of the triangle and four other circles, and the inner circles are always tangent to six other circles. The circles we find in the extended Malfatti problem have the following property: the smallest and largest radii of the circles differ to a great extent. In the study presented herein, we propose algorithms to solve the problem that the tangency properties between the circles and the sides of the triangle are not fixed, so that the number of circles in the triangle is not necessarily a triangle number. The purpose of this change is to attempt to establish the radii of the circles in the triangle within a small range.
Antiviral Activity of Chlorophyll Extracts from Tetraselmis sp., a Marine Microalga, Against Zika Virus Infection
Authors: Kang Nalae , Kim Eun-A , Park Areumi , Heo Seong-Yeong , Heo Jun-Ho , Lee Won-Kyu , Ryu Yong-Kyun , Heo Soo-Jin
Published: September 2024

Recent advancements in the large-scale cultivation of Tetraselmis sp. in Korea have enabled year-round production of this marine microalgae. This study explores the potential industrial applications of Tetraselmis sp. biomass by investigating the antiviral properties of its extracts and primary components. The antiviral effects of Tetraselmis sp. extracts were evaluated in Zika virus (ZIKV)-infected cells. Following extensive isolation and purification, the main compounds were characterized using liquid chromatography–mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) analyses. Their antiviral activities were confirmed using in vitro and in silico tests. Tetraselmis sp. extracts reduced infectious viral particles and non-structural protein 1 messenger RNA levels in ZIKV-infected cells without inducing cytotoxicity. Additionally, they modulated the interferon-mediated immune system responses. Tetraselmis sp. extracts are composed of four main chlorophylls: chlorophyll a, chlorin e6-131-152-dimethyl-173-phytyl ester, hydroxychlorophyll a, and hydroxypheophytin a. Among them, chlorophyll a, chlorin e6-131-152-dimethyl-173-phytyl ester, and hydroxypheophytin showed the antiviral activities in ZIKV-infected cells and molecular docking simulations predicted interactions between these chlorophylls and ZIKV. Our findings suggest that Tetraselmis sp. chlorophyll extracts exert antiviral effects against ZIKV and could serve as potential therapeutic candidates against ZIKV infection.
Preparation and Vasodilation Mechanism of Angiotensin-I-Converting Enzyme Inhibitory Peptide from Ulva prolifera Protein
Authors: Li Zhiyong , He Hongyan , Liu Jiasi , Gu Huiyue , Fu Caiwei , Zeb Aurang , Che Tuanjie , Shen Songdong
Published: September 2024

Ulva prolifera, a type of green algae that can be consumed, was utilized in the production of an angiotensin-I converting enzyme (ACE) inhibitory peptide. The protein from the algae was isolated and subsequently hydrolyzed using a neutral protease. The resulting hydrolysate underwent several processes including Sephadex-G100 filtration chromatography, ultrafiltration, HPLC-Q-TOF-MS analysis, ADMET screening, UV spectrum detection test, molecular docking, and molecular dynamic simulation. Then, the ACE inhibitory peptide named KAF (IC50, 0.63 ± 0.26 µM) was identified. The effectiveness of this peptide in inhibiting ACE can be primarily attributed to two conventional hydrogen bonds. Additionally, it could activate endothelial nitric oxide synthase (eNOS) activity to promote the generation of nitric oxide (NO). Additionally, KAF primarily increased the intracellular calcium (Ca2+) level by acting on L-type Ca2+ channel (LTCC) and the ryanodine receptor (RyR) in the endoplasmic reticulum, and completed the activation of eNOS under the mediation of protein kinase B (Akt) signaling pathway. Our study has confirmed that KAF has the potential to be processed into pharmaceutical candidate functions on vasoconstriction.
Anti-Inflammatory Effect of Fucoidan from Costaria costata Inhibited Lipopolysaccharide-Induced Inflammation in Mice
Authors: Zhang Wei , Lee Peter C. W. , Jin Jun-O
Published: September 2024

Seaweed extracts, especially fucoidan, are well known for their immune-modulating abilities. In this current study, we extracted fucoidan from Costaria costata, a seaweed commonly found in coastal Asia, and examined its anti-inflammatory effect. Fucoidan was extracted from dried C. costata (FCC) using an alcohol extraction method at an extraction rate of 4.5 ± 0.21%. The extracted FCC comprised the highest proportion of carbohydrates, along with sulfate and uronic acid. The immune regulatory effect of FCC was examined using bone marrow-derived dendritic cells (BMDCs). Pretreatment with FCC dose-dependently decreased the lipopolysaccharide (LPS)-induced upregulation of co-stimulatory molecules and major histocompatibility complex. In addition, FCC prevented morphological changes in LPS-induced BMDCs. Moreover, treatment of LPS-induced BMDCs with FCC suppressed the secretion of pro-inflammatory cytokines. In C57BL/6 mice, oral administration of FCC suppressed LPS-induced lung inflammation, reducing the secretion of pro-inflammatory cytokines in the bronchoalveolar lavage fluid. Finally, the administration of FCC suppressed LPS-induced sepsis. Therefore, FCC could be developed as a health supplement based on the observed anti-inflammatory effects.
An Overview of Secondary Metabolites from Soft Corals of the Genus Capnella over the Five Decades: Chemical Structures, Pharmacological Activities, NMR Data, and Chemical Synthesis
Authors: Liu Can-Qi , Yang Qi-Bin , Zhang Ling , Liang Lin-Fu
Published: September 2024

There has been no specific review on the secondary metabolites from soft corals of the genus Capnella till now. In this work, all secondary metabolites from different species of the title genus were described. It covered the first work from 1974 to May 2024, spanning five decades. In the viewpoint of the general structural features, these chemical constituents were classified into four groups: sesquiterpenes, diterpenes, steroids, and lipids. Additionally, the 1H and 13C NMR data of these metabolites were provided when available in the literature. Among them, sesquiterpenes were the most abundant chemical compositions from soft corals of the genus Capnella. A variety of pharmacological activities of these compounds were evaluated, such as cytotoxic, antibacterial, antifungal, and anti-inflammatory activities. In addition, the chemical synthesis works of several representative sesquiterpenes were provided. This review aims to provide an up-to-date knowledge of the chemical structures, pharmacological activities, and chemical synthesis of the chemical constituents from soft corals of the genus Capnella.
Fluid Dynamics Studies on Bottom Liquid Detachment from a Rising Bubble Crossing a Liquid–Liquid Interface
Authors: Cheng Xiangfeng , Qing Gele , Zhao Zhixing , Zhao Baojun
Published: September 2024

The detachment regimes and corresponding detachment height of lower liquid from a coated bubble during the bubble passage through an immiscible liquid–liquid interface were studied. High-speed imaging techniques were used to visualize the lower liquid detachment from a rising bubble near the interface. Analysis of industrial slag samples by a scanning electron microscope (SEM) was also carried out. The results indicate that the detachment height of lower liquid from a rising bubble showed a distinct correlation to penetration regimes. Bubble size and a fluid’s physical properties exerted a significant influence on the detachment height of the lower liquid. The detachment height for medium bubbles (Weber number: 4~4.5; Bond number: 2.5~7.5) varied significantly with increasing bubble size, which contributes to the lower liquid entrainment in the upper phase due, significantly, to the higher detachment height and large entrainment volume. The maximum detachment height for large bubbles is limited to approximately 100 mm due to the early detachment with the liquid column at the interface though large bubbles transporting a larger volume of lower liquid into the upper phase.
Effect of Fe Element and Ultrasonic Vibration on the Microstructure and Mechanical Properties of the Cu-TiB2 Composites
Authors: Zhang Siruo , Li Guanglong , Duan Cunhu , Qu Yingdong , Cheng Min , Dong Shulin
Published: September 2024

Cu-(Fe-Ti)-TiB2 composites were prepared by in situ reaction and vacuum casting with and without ultrasonic vibration. The evolution of the microstructure and mechanical properties of the composite with the variation in Fe element was analyzed. The import of Fe elements could purify the matrix after in situ reaction and the formation of a nanoprecipitated phase, thus improving the strength of Cu-Fe-Ti-TiB2 composites. Meanwhile, compared with the traditional casting process, the Cu-Fe-Ti-TiB2 composites with ultrasonic vibration treatment exhibit uniform TiB2 particle distribution and better properties. The tensile strength and uniform elongation of the composite with a Fe content of 0.7 wt.% reached 511 MPa and 6.02%, increasing by 14.3% and 318% compared to the unalloyed composite, respectively. The tensile strength and uniform elongation of Cu-0.7Fe-Ti-TiB2 composite with ultrasonic vibration treatment increased to 533 MPa and 7.16%, respectively. The TiB2 microscale particles and Fe2Ti nanoscale precipitates with uniform distribution effectively impeded dislocation movement and recrystallization, which improved the tensile strength and stability at elevated temperatures.
Fine-Tuning of Optical Resonance Wavelength of Surface-Micromachined Optical Ultrasound Transducer Arrays for Single-Wavelength Light Source Readout
Authors: Yan Zhiyu , Fang Cheng , Zou Jun
Published: September 2024

This article reports the fine-tuning of the optical resonance wavelength (ORW) of surface-micromachined optical ultrasound transducer (SMOUT) arrays to enable ultrasound data readout with non-tunable interrogation light sources for photoacoustic computed tomography (PACT). Permanent ORW tuning is achieved by material deposition onto or subtraction from the top diaphragm of each element with sub-nanometer resolution. For demonstration, a SMOUT array is first fabricated, and its ORW is tuned for readout with an 808 nm laser diode (LD). Experiments are conducted to characterize the optical and acoustic performances of the elements within the center region of the SMOUT array. Two-dimensional and three-dimensional PACT (photoacoustic computed tomography) is also performed to evaluate the imaging performance of the ORW-tuned SMOUT array. The results show that the ORW tuning does not degrade the optical, acoustic, and overall imaging performances of the SMOUT elements. As a result, the fine-tuning method enables new SMOUT-based PACT systems that are low cost, compact, powerful, and even higher speed, with parallel readout capability.
Operator-Based Triboelectric Nanogenerator Power Management and Output Voltage Control
Authors: Liu Chengyao , Shimane Ryusei , Deng Mingcong
Published: September 2024

In this paper, an operator-based voltage control method for TENGs is investigated, achieving output voltage tracking without compensators and uncertainty suppression using robust right coprime factorization. Initially, a comprehensive simulation-capable circuit model for TENGs is developed, integrating their open-circuit voltage and variable capacitance characteristics. This model is implemented to simulate the behavior of TENGs with a rectifier bridge and capacitive load. To address the high-voltage, low-current pulsating nature of TENG outputs, a storage capacitor switching model is designed to effectively transfer the pulsating energy. This switching model is directly connected to a buck converter and operates under a unified control strategy. A complete TENG power management system was established based on this model, incorporating an operator theory-based control strategy. This strategy ensures steady output voltage under varying load conditions without using compensators, thereby reducing disturbances. Simulation results validate the feasibility of the proposed TENG system and the efficacy of the control strategy, providing a robust framework for optimizing TENG energy harvesting and management systems with significant potential for practical applications.
Chemical Constituents, Anti-Tumor Mechanisms, and Clinical Application: A Comprehensive Review on Scutellaria barbata
Authors: Sun Jiagui , Cao Yuqi , Liu Qiqi , Zhou Zhengshu , Xu Yanan , Liu Chenggang
Published: September 2024

With the increasing global incidence and mortality rates of cancer, the development of novel anti-tumor drugs has become particularly urgent. Scutellaria barbata D. Don, a perennial herb belonging to the genus Scutellaria in the family Lamiaceae, has aroused extensive attention for its medicinal value in recent years. This article presents an exhaustive review of the flavonoid, diterpene, and other chemical constituents harbored within Scutellaria barbata, delving into the intricate mechanisms by which these compounds orchestrate their anti-tumor effects via diverse biological pathways. Remarkably, these compounds distinguish themselves through their capability to regulate cellular signaling, inhibit cancer cell proliferation, trigger apoptosis, disrupt angiogenesis, and bolster immune responses. These anti-tumor effects are achieved through strategic modulation of pivotal signaling cascades, particularly the PI3K/Akt/mTOR, MAPK, and NFκB pathways. In addition, this article also summarizes the clinical applications of Scutellaria barbata in tumor treatment, especially its potential in alleviating the side effects of radiotherapy and chemotherapy and improving patients’ quality of life. In conclusion, this review comprehensively summarizes and analyzes the chemical constituents, anti-tumor mechanisms, and clinical applications of Scutellaria barbata, with the aim of systematically reviewing the existing research results and exploring potential future research directions.
Neuroprotective Effect of Flavonoid Agathisflavone in the Ex Vivo Cerebellar Slice Neonatal Ischemia
Authors: Carreira Rodrigo Barreto , dos Santos Cleonice Creusa , de Oliveira Juciele Valeria Ribeiro , da Silva Victor Diogenes Amaral , David Jorge Maurício , Butt Arthur Morgan , Costa Silvia Lima
Published: September 2024

Agathisflavone is a flavonoid that exhibits anti-inflammatory and anti-oxidative properties. Here, we investigated the neuroprotective effects of agathisflavone on central nervous system (CNS) neurons and glia in the cerebellar slice ex vivo model of neonatal ischemia. Cerebellar slices from neonatal mice, in which glial fibrillary acidic protein (GFAP) and SOX10 drive expression of enhanced green fluorescent protein (EGFP), were used to identify astrocytes and oligodendrocytes, respectively. Agathisflavone (10 μM) was administered preventively for 60 min before inducing ischemia by oxygen and glucose deprivation (OGD) for 60 min and compared to controls maintained in normal oxygen and glucose (OGN). The density of SOX-10+ oligodendrocyte lineage cells and NG2 immunopositive oligodendrocyte progenitor cells (OPCs) were not altered in OGD, but it resulted in significant oligodendroglial cell atrophy marked by the retraction of their processes, and this was prevented by agathisflavone. OGD caused marked axonal demyelination, determined by myelin basic protein (MBP) and neurofilament (NF70) immunofluorescence, and this was blocked by agathisflavone preventative treatment. OGD also resulted in astrocyte reactivity, exhibited by increased GFAP-EGFP fluorescence and decreased expression of glutamate synthetase (GS), and this was prevented by agathisflavone pretreatment. In addition, agathisflavone protected Purkinje neurons from ischemic damage, assessed by calbindin (CB) immunofluorescence. The results demonstrate that agathisflavone protects neuronal and myelin integrity in ischemia, which is associated with the modulation of glial responses in the face of ischemic damage.
Ultrafast Laser Processing for High-Aspect-Ratio Structures
Authors: Qin Muyang , Zhao Xinjing , Fan Hanyue , Leng Ruizhe , Yu Yanhao , Li Aiwu , Gao Bingrong
Published: September 2024

Over the past few decades, remarkable breakthroughs and progress have been achieved in ultrafast laser processing technology. Notably, the remarkable high-aspect-ratio processing capabilities of ultrafast lasers have garnered significant attention to meet the stringent performance and structural requirements of materials in specific applications. Consequently, high-aspect-ratio microstructure processing relying on nonlinear effects constitutes an indispensable aspect of this field. In the paper, we review the new features and physical mechanisms underlying ultrafast laser processing technology. It delves into the principles and research achievements of ultrafast laser-based high-aspect-ratio microstructure processing, with a particular emphasis on two pivotal technologies: filamentation processing and Bessel-like beam processing. Furthermore, the current challenges and future prospects for achieving both high precision and high aspect ratios simultaneously are discussed, aiming to provide insights and directions for the further advancement of high-aspect-ratio processing.
Ratiometric Fluorescent pH Sensing with Carbon Dots: Fluorescence Mapping across pH Levels for Potential Underwater Applications
Authors: Szapoczka Wiktoria Karolina , Olla Chiara , Carucci Cristina , Truskewycz Adam Leo , Skodvin Tore , Salis Andrea , Carbonaro Carlo Maria , Holst Bodil , Thomas Peter James
Published: September 2024

Ocean acidification has become a major climate change concern requiring continuous observation. Additionally, in the industry, pH surveillance is of great importance. Consequently, there is a pressing demand to develop robust and inexpensive pH sensors. Ratiometric fluorescence pH sensing stands out as a promising concept. The application of carbon dots in fluorescent sensing presents a compelling avenue for the advancement of pH-sensing solutions. This potential is underpinned by the affordability of carbon dots, their straightforward manufacturing process, low toxicity, and minimal susceptibility to photobleaching. Thus, investigating novel carbon dots is essential to identify optimal pH-sensitive candidates. In this study, five carbon dots were synthesized through a simple solvothermal treatment, and their fluorescence was examined as a function of pH within the range of 5–9, across an excitation range of 200–550 nm and an emission range of 250–750 nm. The resulting optical features showed that all five carbon dots exhibited pH sensitivity in both the UV and visible regions. One type of carbon dot, synthesized from m-phenylenediamine, displayed ratiometric properties at four excitation wavelengths, with the best results observed when excited in the visible spectrum at 475 nm. Indeed, these carbon dots exhibited good linearity over pH values of 6–9 in aqueous Carmody buffer solution by calculating the ratio of the green emission band at 525 nm to the orange one at 630 nm (I525nm/I630nm), demonstrating highly suitable properties for ratiometric sensing.
Matcha Does Not Affect Electroencephalography during Sleep but May Enhance Mental Well-Being: A Randomized Placebo-Controlled Clinical Trial
Authors: Baba Yoshitake , Takihara Takanobu , Okamura Noritaka
Published: September 2024

Although theanine in matcha improves sleep quality and cognitive function, the caffeine in green tea is thought to worsen sleep quality. Therefore, this study investigated the factors behind the observed improvements in subjective sleep quality in matcha. A placebo-controlled randomized double-blind parallel-group study was conducted on healthy Japanese men and women aged 27–64 years. After 4 weeks of consuming 2.7 g of matcha daily (containing 50.3 mg theanine, 301.4 mg catechins, and 71.5 mg caffeine), no significant differences were observed between the control and matcha groups on total sleep time, sleep latency, wake after sleep onset, or sleep efficiency measured by electroencephalography (EEG). However, the sleep questionnaire Oguri–Shirakawa–Azumi Sleep Inventory, the Middle-age and Aged version (OSA-MA), administered immediately after waking showed a trend toward increased satisfaction with sleep time (p < 0.1), and EEG measurements indicated significantly shortened wake-up times after waking with matcha intake (p < 0.05). The Beck Depression Inventory-II scores also tended to decrease (p < 0.1). The continuous intake of matcha may offer improved subjective sleep quality and emotional stability despite not offering significant changes in objective sleep parameters.
Nutrient Analysis of Raw and Cooked USDA Prime Beef Cuts
Authors: Mortensen Emma G. , Fuerniss Hannah F. , Legako Jerrad F. , Thompson Leslie D. , Woerner Dale R.
Published: September 2024

Nutrient composition data that accurately represent available beef products are critical to understanding beef’s role in healthy dietary patterns. The quality of beef products has changed over the past several decades, and updated nutrient data are warranted as USDA Prime beef cuts become more available. In an effort to provide a complete nutrient profile for frequently purchased USDA Prime beef cuts, five USDA Prime cuts; strip loin steak, tenderloin steak, ribeye steak, top sirloin steak, and rib roast were collected from retail stores in six geographical locations over three collections for macro- and micronutrient analysis in both the raw and cooked state. The separable lean portion of all analyzed USDA Prime cuts qualified as a good or excellent source, providing 10–19% or at least 20% of the daily value, respectively, for protein, niacin, vitamin B12, selenium, phosphorus, and zinc per FDA labeling claim standards. There was not a significant difference in cholesterol content between any of the cuts, raw or cooked (p ≥ 0.44 and 0.34, respectively). The percent lipid in raw, separable lean portions of the rib roast and strip loin steak was significantly greater than the lipid portion in tenderloin and top sirloin steaks (p ≤ 0.01). Per USDA standards, the separable lean portions of tenderloin steak and top sirloin steak qualify as lean beef, containing less than 10 g total fat, less than 4.5 g saturated fat, and less than or equal to 95 mg cholesterol. The current study provides the most up-to-date nutrient analysis for USDA Prime beef cuts, helping consumers and health professionals better identify the role of high-quality beef cuts in healthy dietary patterns.
Impact of Coffee Intake on Measures of Wellbeing in Mice
Authors: Machado Nuno J. , Ardais Ana Paula , Nunes Ana , Szabó Eszter C. , Silveirinha Vasco , Silva Henrique B. , Kaster Manuella P. , Cunha Rodrigo A.
Published: September 2024

Coffee intake is increasingly recognized as a life-style factor associated with the preservation of health, but there is still a debate on the relative effects of caffeinated and decaffeinated coffee. We now tested how the regular drinking of caffeinated and decaffeinated coffee for 3 weeks impacted on the behavior of male and female adult mice. Males drinking caffeinated coffee displayed statistically significant lower weight gain, increased sensorimotor coordination, greater motivation in the splash test, more struggling in the forced swimming test, faster onset of nest building, more marble burying and greater sociability. Females drinking caffeinated coffee displayed statistically significant increased hierarchy fighting, greater self-care and motivation in the splash test and faster onset of nest building. A post-hoc two-way ANOVA revealed sex-differences in the effects of caffeinated coffee (p values for interaction between the effect of caffeinated coffee and sex) on the hierarchy in the tube test (p = 0.044; dominance), in the time socializing (p = 0.044) and in the latency to grooming (p = 0.048; selfcare), but not in the marble burying test (p = 0.089). Intake of decaffeinated coffee was devoid of effects in males and females. Since caffeine targets adenosine receptors, we verified that caffeinated but not decaffeinated coffee intake increased the density of adenosine A1 receptors (A1R) and increased A1R-mediated tonic inhibition of synaptic transmission in the dorsolateral striatum and ventral but not dorsal hippocampus, the effects being more evident in the ventral hippocampus of females and striatum of males. In contrast, caffeinated and decaffeinated coffee both ameliorated the antioxidant status in the frontal cortex. It is concluded that caffeinated coffee increases A1R-mediated inhibition in mood-related areas bolstering wellbeing of both males and females, with increased sociability in males and hierarchy struggling and self-care in females.
Precision or Personalized Nutrition: A Bibliometric Analysis
Authors: Hinojosa-Nogueira Daniel , Subiri-Verdugo Alba , Díaz-Perdigones Cristina Mª , Rodríguez-Muñoz Alba , Vilches-Pérez Alberto , Mela Virginia , Tinahones Francisco J. , Moreno-Indias Isabel
Published: September 2024

Food systems face the challenge of maintaining adequate nutrition for all populations. Inter-individual responses to the same diet have made precision or personalized nutrition (PN) an emerging and relevant topic. The aim of this study is to analyze the evolution of the PN field, identifying the principal actors and topics, and providing a comprehensive overview. Therefore, a bibliometric analysis of the scientific research available through the Web of Science (WOS) database was performed, revealing 2148 relevant papers up to June 2024. VOSviewer and the WOS platform were employed for the processing and analysis, and included an evaluation of diverse data such as country, author or most frequent keywords, among others. The analysis revealed a period of exponential growth from 2015 to 2023, with the USA, Spain, and England as the top contributors. The field of “Nutrition and Dietetics” is particularly significant, comprising nearly 33% of the total publications. The most highly cited institutions are the universities of Tufts, College Dublin, and Navarra. The relationship between nutrition, genetics, and omics sciences, along with dietary intervention studies, has been a defining factor in the evolution of PN. In conclusion, PN represents a promising field of research with significant potential for further advancement and growth.
Comparative Chemical Analysis and Bioactive Properties of Aqueous and Glucan-Rich Extracts of Three Widely Appreciated Mushrooms: Agaricus bisporus (J.E.Lange) Imbach, Laetiporus sulphureus (Bull.) Murill and Agrocybe aegerita (V. Brig.) Vizzini
Authors: Petrović Jovana , Glamočlija Jasmina , Milinčić Danijel D. , Doroški Ana , Lević Steva , Stanojević Slađana P. , Kostić Aleksandar Ž. , Minić Dušanka A. Popović , Vidović Bojana B. , Plećić Ana , Nedović Viktor A. , Pešić Mirjana B. , Stojković Dejan
Published: September 2024

Herein we describe the antioxidant, antimicrobial, antibiofilm, anti-inflammatory and wound-healing potential of aqueous and polysaccharide extracts from three widely appreciated mushrooms: Agrocybe aegerita, Laetiporus sulphureus and Agaricus bisporus. Moreover, we present their detailed phenolic, polysaccharide and protein profiles and ATR-FTIR spectra. The study found that polysaccharide extracts (PEs) from mushrooms had higher total and β-glucan levels than aqueous extracts (AEs), with A. aegerita showing the highest content. L. sulphureus had a higher total protein content, and A. aegerita AE had the highest phenolic content. Our results indicate that all the tested extracts have high potential regarding their bioactive properties, with A. aegerita being the most promising one. Namely, the antibacterial activity assay showed that the development of the skin-infection-causing agent, Staphylococcus aureus, was inhibited with a minimal inhibitory concentration of 4.00 mg/mL and minimal bactericidal concentration of 8.00 mg/mL, while the results regarding wound healing showed that, over the course of 24 h, the A. aegerita extract actively promoted wound closure in the HaCaT keratinocyte cell line model. The anti-inflammatory activity results clearly showed that when we used S. aureus as an inflammation-inducing agent and the A. aegerita aqueous extract in treatment, IL-6 levels reduced to the level of 4.56 pg/mL. The obtained data suggest that the tested mushroom extracts may serve as a source of bioactive compounds, with potential applications in the cosmeceutical, pharmaceutical and food industries. Furthermore, potential skin preparations carefully crafted with mushroom extract may help restore the skin’s barrier function, decrease the probability of staph infections and minimize skin irritation.
Synthesis of New Thiazole-Privileged Chalcones as Tubulin Polymerization Inhibitors with Potential Anticancer Activities
Authors: Hashem Hamada , Hassan Abdelfattah , Abdelmagid Walid M. , Habib Ahmed G. K. , Abdel-Aal Mohamed A. A. , Elshamsy Ali M. , El Zawily Amr , Radwan Ibrahim Taha , Bräse Stefan , Abdel-Samea Ahmed S. , Rabea Safwat M.
Published: September 2024

A series of novel thiazole-based chalcones were evaluated for their anticancer activity as potential tubulin polymerization inhibitors. In vitro anticancer screening for the thiazole derivatives 2a–2p exhibited broad-spectrum antitumor activity against various cancer cell lines particularly Ovar-3 and MDA-MB-468 cells with a GI50 range from 1.55 to 2.95 μΜ, respectively. Compound 2e demonstrated significant inhibition of tubulin polymerization, with an IC50 value of 7.78 μM compared to Combretastatin-A4 (CA-4), with an IC50 value of 4.93 μM. Molecular docking studies of compounds 2e, 2g, and 2h into tubulin further supported these findings, revealing that they bind effectively to the colchicine binding site, mirroring key interactions exhibited by CA-4. Computational predictions suggested favorable oral bioavailability and drug-likeness for these compounds, highlighting their potential for further development as chemotherapeutic agents.
TnP and AHR-CYP1A1 Signaling Crosstalk in an Injury-Induced Zebrafish Inflammation Model
Authors: Disner Geonildo Rodrigo , Fernandes Thales Alves de Melo , Nishiyama-Jr Milton Yutaka , Lima Carla , Wincent Emma , Lopes-Ferreira Monica
Published: September 2024

Aryl Hydrocarbon Receptor (AHR) signaling is crucial for regulating the biotransformation of xenobiotics and physiological processes like inflammation and immunity. Meanwhile, Thalassophryne nattereri Peptide (TnP), a promising anti-inflammatory candidate from toadfish venom, demonstrates therapeutic effects through immunomodulation. However, its influence on AHR signaling remains unexplored. This study aimed to elucidate TnP’s molecular mechanisms on the AHR–cytochrome P450, family 1 (CYP1) pathway upon injury-induced inflammation in wild-type (WT) and Ahr2-knockdown (KD) zebrafish larvae through transcriptomic analysis and Cyp1a reporters. TnP, while unable to directly activate AHR, potentiated AHR activation by the high-affinity ligand 6-Formylindolo [3,2-b]carbazole (FICZ), implying a role as a CYP1A inhibitor, confirmed by in vitro studies. This interplay suggests TnP’s ability to modulate the AHR-CYP1 complex, prompting investigations into its influence on biotransformation pathways and injury-induced inflammation. Here, the inflammation model alone resulted in a significant response on the transcriptome, with most differentially expressed genes (DEGs) being upregulated across the groups. Ahr2-KD resulted in an overall greater number of DEGs, as did treatment with the higher dose of TnP in both WT and KD embryos. Genes related to oxidative stress and inflammatory response were the most apparent under inflamed conditions for both WT and KD groups, e.g., Tnfrsf1a, Irf1b, and Mmp9. TnP, specifically, induces the expression of Hspa5, Hsp90aa1.2, Cxcr3.3, and Mpeg1.2. Overall, this study suggests an interplay between TnP and the AHR-CYP1 pathway, stressing the inflammatory modulation through AHR-dependent mechanisms. Altogether, these results may offer new avenues in novel therapeutic strategies, such as based on natural bioactive molecules, harnessing AHR modulation for targeted and sustained drug effects in inflammatory conditions.
Pharmacological Prescription at the End of Life: Quality Assessment in the Transition of Care to a Community Palliative Care Support Team
Authors: Rodrigues Inês , Ribeiro Hugo , Costa Carolina , Rocha-Neves João , Dourado Marília
Published: September 2024

Appropriate pharmacological management is a cornerstone of quality in palliative care (PC), focusing on comfort and quality of life. Therapeutic review is crucial in PC, aiming to optimize symptom relief, reduce adverse effects, and manage drug interactions. This study aims to delve into the real-world pharmacological prescription practices within a Community Palliative Care Support Team (CPCST) in the northern region of Portugal, comparing practices at admission and at the last consultation before death. It is an observational, cross-sectional, retrospective study without intervention involving patients admitted to a CPCST in 2021. Data were obtained from clinical records, and the statistical analysis included descriptive and inferential measures. Sixty-four patients were included, with an average age of 77.34 years, referred mainly by a specialized Hospital Palliative Care Support Team (65.63%). Polypharmacy was present, with a significant increase in opioids, antipsychotics, prokinetics, antiemetics, antispasmodics, and local corticosteroids, and a reduction in drugs for peptic ulcer and gastroesophageal reflux treatment, antithrombotics, hypolipidemics, antihypertensives, and antidiabetics, among others. The oral route was preferred, decreasing between the two analyzed moments (85.5% versus 49.1%). Pro re nata (PRN) medications increased significantly (p ≤ 0.001). The prescription profile reflects a focus on symptom relief. The deprescription of drugs for chronic comorbidities suggests adaptation to care goals. At the end of life, PRN medications increase significantly (1.34 versus 3.26, p ≤ 0.001), administered as needed to soothe fluctuating symptoms. The pharmacological classes that have significantly increased are relevant in alleviating common symptoms in PC. The use of alternative routes for medication administration increases as instability of the oral route occurs, leading to a reduction in orally administered medications. Among these alternatives, the subcutaneous route shows the largest increase. The findings underscore the importance of flexible and responsive medication strategies in end-of-life care.
Recent Advances in Drug Delivery Strategies for High-Risk BCG-Unresponsive Non-Muscle Invasive Bladder Cancer: A Brief Review from 2018 to 2024
Authors: Qu Feng , Darji Saloni , Thompson David H.
Published: September 2024

High-risk BCG-unresponsive non-muscle invasive bladder cancer (NMIBC) is a condition that is typically treated with Bacillus Calmette–Guérin (BCG) therapy. Unfortunately, NMIBC is characterized by high recurrence, with a significant percentage of BCG patients ultimately requiring radical cystectomy. As a consequence, the development of effective new therapies to avoid RC has become a rapidly evolving field to address this unmet clinical need. To date, three biologics—Keytruda, Adstiladrin, and Anktiva—have been approved by the FDA, and multiple drug modalities, particularly gene therapies, have shown promising results in clinical trials. Advances in drug delivery strategies, such as targeted delivery, sustained release, and permeabilization of protective layers, are critical in overcoming the challenges posed by therapeutic intervention in bladder cancer. This review focuses on high-risk BCG-unresponsive NMIBC therapies that have been or are currently being investigated in clinical trials, offering a broad overview of the delivery system designs and up-to-date clinical outcomes that have been reported as of July 2024. It aims to inform the development of future drug delivery systems for second-line therapies in high-risk BCG-unresponsive NMIBC.
Can We Simplify Liposome Manufacturing Using a Complex DoE Approach?
Authors: Lindsay Sarah , Tumolva Olympia , Khamiakova Tatsiana , Coppenolle Hans , Kovarik Martin , Shah Sanket , Holm René , Perrie Yvonne
Published: September 2024

Microfluidic liposome production presents a streamlined pathway for expediting the translation of liposomal formulations from the laboratory setting to clinical applications. Using this production method, resultant liposome characteristics can be tuned through the control of both the formulation parameters (including the lipids and solvents used) and production parameters (including the production speed and mixing ratio). Therefore, the aim of this study was to investigate the relationship between not only total flow rate (TFR), the fraction of the aqueous flow rate over the organic flow rate (flow rate ratio (FRR)), and the lipid concentration, but also the solvent selection, aqueous buffer, and production temperature. To achieve this, we used temperature, applying a design of experiment (DoE) combined with machine learning. This study demonstrated that liposome size and polydispersity were influenced by manipulation of not only the total flow rate and flow rate ratio but also through the lipids, lipid concentration, and solvent selection, such that liposome attributes can be in-process controlled, and all factors should be considered within a manufacturing process as impacting on liposome critical quality attributes.
Kierkegaard’s Lesson on Religious Conformism vs. the Current Mainstream Environmentalism
Authors: Tavilla Igor
Published: September 2024

This paper aims to show how Kierkegaard’s attack upon Christendom still works today to contrast current forms of conformism disguised under the appearance of new secular religions. I will start with considering Kierkegaard’s concept of conformism as a form of despair. As such, conformism is incompatible with Christianity, as well as with the development of a true Self. Secondly, I will focus on the current religious scene in Western Europe. While Christianity has become a minority in society, new secular religions have arisen and, along with them, new compelling narratives. Mainstream environmentalism appears to be one of these. Finally, I will try to show how Kierkegaard’s arguments against Christendom can be also applied to environmental propaganda.