bluuebunny/crossref_metadata_2025 huggingface dataset:
Natural Bioactive Cinnamoyltyramine Alkylamides and Co-Metabolites
Authors: Evidente Antonio , Masi Marco
Published: November 2021

Natural products are a vital source for agriculture, medicine, cosmetic and other fields. Among them alkylamides are a broad and expanding group found in at least 33 plant families. Frequently, they possess a simple carbon skeleton architecture but show broad structural variability and important properties such as immunomodulatory, antimicrobial, antiviral, larvicidal, insecticidal and antioxidant properties, amongst others. Despite to these several and promising biological activities, up to today, only two reviews have been published on natural alkylamides. One focuses on their potential pharmacology application and their distribution in the plant kingdom and the other one on the bioactive alkylamides specifically found in Annona spp. The present review is focused on the plant bioactive cinnamoyltyramine alkylamides, which are subject of several works reported in the literature. Furthermore, the co-metabolites isolated from the same natural sources and their biological activities are also reported.
Amazonian Bacuri (Platonia insignis Mart.) Fruit Waste Valorisation Using Response Surface Methodology
Authors: Yamaguchi Klenicy K. L. , Dias David S. , Lamarão Carlos Victor , Castelo Karen F. A. , Lima Max S. , Antonio Ananda S. , Converti Attilio , Lima Emerson S. , Veiga-Junior Valdir F.
Published: November 2021

Bacuri (Platonia insignis Mart) is a species from the Clusiaceae genus. Its fruit pulp is commonly used in South America in several food products, such as beverages, ice cream and candies. Only the pulp of the fruit is used, and the peels and seeds are considered waste from these industries. As a trioxygenated xanthone source, this species is of high interest for bioproduct development. This work evaluated the mesocarp and epicarp of bacuri fruits through different extraction methods and experimental conditions (pH, temperature and solvent) in order to determine the most effective method for converting this agro-industrial waste in a value-added bioproduct. Open-column procedures and HPLC and NMR experiments were performed to evaluate the chemical composition of the extracts, along with total phenols, total flavonoids and antioxidant activities (sequestration of the DPPH and ABTS radicals). A factorial design and response surface methodology were used. The best extraction conditions of substances with antioxidant properties were maceration at 50 °C with 100% ethanol as solvent for mesocarp extracts, and acidic sonication in 100% ethanol for epicarp extracts, with an excellent phenolic profile and antioxidant capacities. The main compounds isolated were the prenylated benzophenones garcinielliptone FC (epicarp) and 30-epi-cambogin (mesocarp). This is the first study analysing the performance of extraction methods within bacuri agro-industrial waste. Results demonstrated that shells and seeds of bacuri can be used as phenolic-rich bioproducts obtained by a simple extraction method, increasing the value chain of this fruit.
Ethyl Pyruvate Ameliorates Experimental Autoimmune Myocarditis
Authors: Gajić Dragica , Despotović Sanja , Koprivica Ivan , Miljković Đorđe , Saksida Tamara
Published: November 2021

Ethyl pyruvate (EP) has profound anti-inflammatory and immunomodulatory properties. Here, its effects were determined on experimental autoimmune myocarditis (EAM) induced in mice by heart-specific myosin-alpha heavy chain peptide immunization. EP was applied intraperitoneally, daily, starting with the immunization. Severity of EAM was determined by histological assessment of immune cell infiltrates into the heart. Cells were phenotypically characterized by flow cytometry. Concentration of cytokines in cell culture supernatants and sera was determined by ELISA. EP reduced the infiltration of immune cells into the heart and lessened heart inflammation. Smaller number of total immune cells, as well as of CD11b+ and CD11c+ cells were isolated from the hearts of EP-treated mice. A reduced number of antigen-presenting cells, detected by anti-CD11c, MHC class II and CD86 antibodies, as well as of T helper (Th)1 and Th17 cells, detected by anti-CD4, IFN-γ and IL-17 antibodies, was determined in mediastinal lymph nodes draining the heart, in parallel. In the spleen, only the number of CD11c+ cells were reduced, but not of the other examined populations, thus implying limited systemic effect of EP. Reduced production of IFN-γ and IL-17 by myosin-alpha heavy chain peptide-restimulated cells of the lymph nodes draining the site of immunization was observed in EP-treated mice. Our results clearly imply that EP restrains autoimmunity in EAM. Therapeutic application of EP in the treatment of myocarditis in humans should be addressed in the forthcoming studies.
Deepening into Intracellular Signaling Landscape through Integrative Spatial Proteomics and Transcriptomics in a Lymphoma Model
Authors: Landeira-Viñuela Alicia , Díez Paula , Juanes-Velasco Pablo , Lécrevisse Quentin , Orfao Alberto , De Las Rivas Javier , Fuentes Manuel
Published: November 2021

Human Proteome Project (HPP) presents a systematic characterization of the protein landscape under different conditions using several complementary-omic techniques (LC-MS/MS proteomics, affinity proteomics, transcriptomics, etc.). In the present study, using a B-cell lymphoma cell line as a model, comprehensive integration of RNA-Seq transcriptomics, MS/MS, and antibody-based affinity proteomics (combined with size-exclusion chromatography) (SEC-MAP) were performed to uncover correlations that could provide insights into protein dynamics at the intracellular level. Here, 5672 unique proteins were systematically identified by MS/MS analysis and subcellular protein extraction strategies (neXtProt release 2020-21, MS/MS data are available via ProteomeXchange with identifier PXD003939). Moreover, RNA deep sequencing analysis of this lymphoma B-cell line identified 19,518 expressed genes and 5707 protein coding genes (mapped to neXtProt). Among these data sets, 162 relevant proteins (targeted by 206 antibodies) were systematically analyzed by the SEC-MAP approach, providing information about PTMs, isoforms, protein complexes, and subcellular localization. Finally, a bioinformatic pipeline has been designed and developed for orthogonal integration of these high-content proteomics and transcriptomics datasets, which might be useful for comprehensive and global characterization of intracellular protein profiles.
Selective Biological Effects of Selenium-Enriched Polysaccharide (Se-Le-30) Isolated from Lentinula edodes Mycelium on Human Immune Cells
Authors: Kaleta Beata , Roszczyk Aleksander , Zych Michał , Kniotek Monika , Zagożdżon Radosław , Klimaszewska Marzenna , Malinowska Eliza , Pac Michał , Turło Jadwiga
Published: November 2021

A common edible mushroom Lentinula edodes, is an important source of numerous biologically active substances, including polysaccharides, with immunomodulatory and antitumor properties. In the present work, the biological activity of the crude, homogenous (Se)-enriched fraction (named Se-Le-30), which has been isolated from L. edodes mycelium by a modified Chihara method towards human peripheral blood mononuclear cells (PBMCs) and peripheral granulocytes, was investigated. The Se-Le-30 fraction, an analog of lentinan, significantly inhibited the proliferation of human PBMCs stimulated with anti-CD3 antibodies or allostimulated, and down-regulated the production of tumor necrosis factor (TNF)-α by CD3+ T cells. Moreover, it was found that Se-Le-30 significantly reduced the cytotoxic activity of human natural killer (NK) cells. The results suggested the selective immunosuppressive activity of this fraction, which is non-typical for mushroom derived polysaccharides.
Role of Store-Operated Ca2+ Entry in the Pulmonary Vascular Remodeling Occurring in Pulmonary Arterial Hypertension
Authors: Masson Bastien , Montani David , Humbert Marc , Capuano Véronique , Antigny Fabrice
Published: November 2021

Pulmonary arterial hypertension (PAH) is a severe and multifactorial disease. PAH pathogenesis mostly involves pulmonary arterial endothelial and pulmonary arterial smooth muscle cell (PASMC) dysfunction, leading to alterations in pulmonary arterial tone and distal pulmonary vessel obstruction and remodeling. Unfortunately, current PAH therapies are not curative, and therapeutic approaches mostly target endothelial dysfunction, while PASMC dysfunction is under investigation. In PAH, modifications in intracellular Ca2+ homoeostasis could partly explain PASMC dysfunction. One of the most crucial actors regulating Ca2+ homeostasis is store-operated Ca2+ channels, which mediate store-operated Ca2+ entry (SOCE). This review focuses on the main actors of SOCE in human and experimental PASMC, their contribution to PAH pathogenesis, and their therapeutic potential in PAH.
Transcription Factors Active in the Anterior Blastema of Schmidtea mediterranea
Authors: Suzuki-Horiuchi Yoko , Schmitz Henning , Barlassina Carlotta , Eccles David , Sinn Martina , Ortmeier Claudia , Moritz Sören , Gentile Luca
Published: November 2021

Regeneration, the restoration of body parts after injury, is quite widespread in the animal kingdom. Species from virtually all Phyla possess regenerative abilities. Human beings, however, are poor regenerators. Yet, the progress of knowledge and technology in the fields of bioengineering, stem cells, and regenerative biology have fostered major advancements in regenerative medical treatments, which aim to regenerate tissues and organs and restore function. Human induced pluripotent stem cells can differentiate into any cell type of the body; however, the structural and cellular complexity of the human tissues, together with the inability of our adult body to control pluripotency, require a better mechanistic understanding. Planarians, with their capacity to regenerate lost body parts thanks to the presence of adult pluripotent stem cells could help providing such an understanding. In this paper, we used a top-down approach to shortlist blastema transcription factors (TFs) active during anterior regeneration. We found 44 TFs—31 of which are novel in planarian—that are expressed in the regenerating blastema. We analyzed the function of half of them and found that they play a role in the regeneration of anterior structures, like the anterior organizer, the positional instruction muscle cells, the brain, the photoreceptor, the intestine. Our findings revealed a glimpse of the complexity of the transcriptional network governing anterior regeneration in planarians, confirming that this animal model is the perfect playground to study in vivo how pluripotency copes with adulthood.
EmbedDTI: Enhancing the Molecular Representations via Sequence Embedding and Graph Convolutional Network for the Prediction of Drug-Target Interaction
Authors: Jin Yuan , Lu Jiarui , Shi Runhan , Yang Yang
Published: November 2021

The identification of drug-target interaction (DTI) plays a key role in drug discovery and development. Benefitting from large-scale drug databases and verified DTI relationships, a lot of machine-learning methods have been developed to predict DTIs. However, due to the difficulty in extracting useful information from molecules, the performance of these methods is limited by the representation of drugs and target proteins. This study proposes a new model called EmbedDTI to enhance the representation of both drugs and target proteins, and improve the performance of DTI prediction. For protein sequences, we leverage language modeling for pretraining the feature embeddings of amino acids and feed them to a convolutional neural network model for further representation learning. For drugs, we build two levels of graphs to represent compound structural information, namely the atom graph and substructure graph, and adopt graph convolutional network with an attention module to learn the embedding vectors for the graphs. We compare EmbedDTI with the existing DTI predictors on two benchmark datasets. The experimental results show that EmbedDTI outperforms the state-of-the-art models, and the attention module can identify the components crucial for DTIs in compounds.
A Combined Spectroscopic and In Silico Approach to Evaluate the Interaction of Human Frataxin with Mitochondrial Superoxide Dismutase
Authors: Doni Davide , Meggiolaro Marta , Santos Javier , Audran Gérard , Marque Sylvain R. A. , Costantini Paola , Bortolus Marco , Carbonera Donatella
Published: November 2021

Frataxin (FXN) is a highly conserved mitochondrial protein whose deficiency causes Friedreich’s ataxia, a neurodegenerative disease. The precise physiological function of FXN is still unclear; however, there is experimental evidence that the protein is involved in biosynthetic iron–sulfur cluster machinery, redox imbalance, and iron homeostasis. FXN is synthesized in the cytosol and imported into the mitochondria, where it is proteolytically cleaved to the mature form. Its involvement in the redox imbalance suggests that FXN could interact with mitochondrial superoxide dismutase (SOD2), a key enzyme in antioxidant cellular defense. In this work, we use site-directed spin labelling coupled to electron paramagnetic resonance spectroscopy (SDSL-EPR) and fluorescence quenching experiments to investigate the interaction between human FXN and SOD2 in vitro. Spectroscopic data are combined with rigid body protein–protein docking to assess the potential structure of the FXN-SOD2 complex, which leaves the metal binding region of FXN accessible to the solvent. We provide evidence that human FXN interacts with human SOD2 in vitro and that the complex is in fast exchange. This interaction could be relevant during the assembly of iron-sulfur (FeS) clusters and/or their incorporation in proteins when FeS clusters are potentially susceptible to attacks by reactive oxygen species.
Neuromodulation Induced by Sitagliptin: A New Strategy for Treating Diabetic Retinopathy
Authors: Ramos Hugo , Bogdanov Patricia , Sabater David , Huerta Jordi , Valeri Marta , Hernández Cristina , Simó Rafael
Published: November 2021

Diabetic retinopathy (DR) involves progressive neurovascular degeneration of the retina. Reduction in synaptic protein expression has been observed in retinas from several diabetic animal models and human retinas. We previously reported that the topical administration (eye drops) of sitagliptin, a dipeptidyl peptidase-4 (DPP-4) inhibitor, prevented retinal neurodegeneration induced by diabetes in db/db mice. The aim of the present study is to examine whether the modulation of presynaptic proteins is a mechanism involved in the neuroprotective effect of sitagliptin. For this purpose, 12 db/db mice, aged 12 weeks, received a topical administration of sitagliptin (5 μL; concentration: 10 mg/mL) twice per day for 2 weeks, while other 12 db/db mice were treated with vehicle (5 μL). Twelve non-diabetic mice (db/+) were used as a control group. Protein levels were assessed by western blot and immunohistochemistry (IHC), and mRNA levels were evaluated by reverse transcription polymerase chain reaction (RT-PCR). Our results revealed a downregulation (protein and mRNA levels) of several presynaptic proteins such as synapsin I (Syn1), synaptophysin (Syp), synaptotagmin (Syt1), syntaxin 1A (Stx1a), vesicle-associated membrane protein 2 (Vamp2), and synaptosomal-associated protein of 25 kDa (Snap25) in diabetic mice treated with vehicle in comparison with non-diabetic mice. These proteins are involved in vesicle biogenesis, mobilization and docking, membrane fusion and recycling, and synaptic neurotransmission. Sitagliptin was able to significantly prevent the downregulation of all these proteins. We conclude that sitagliptin exerts beneficial effects in the retinas of db/db mice by preventing the downregulation of crucial presynaptic proteins. These neuroprotective effects open a new avenue for treating DR as well other retinal diseases in which neurodegeneration/synaptic abnormalities play a relevant role.
Selective Anticancer and Antimicrobial Metallodrugs Based on Gold(III) Dithiocarbamate Complexes
Authors: Abás Elisa , Aguirre-Ramírez Diego , Laguna Mariano , Grasa Laura
Published: November 2021

New dithiocarbamate cycloaurated complexes have been synthesized and their physicochemical and in vitro antitumor properties have been evaluated. All the performed studies highlighted good transport through the blood and biodistribution, according to the balance between the properties of hydrophilicity/lipophilicity and the binding of moderate strength to the BSA protein. Furthermore, none of the complexes exhibited reduction or decomposition reactions, presenting excellent physiological stability. The in vitro cytotoxic effect was evaluated on human colon cancer cell line Caco-2/TC7, and the complexes showed great antiproliferative activity and excellent selectivity, as much less effect was detected on normal Caco-2/TC7 cells. Most of the complexes exhibit antiproliferative activity that was better than or similar to auranofin, and at least nine times better than that of cisplatin. Its action mechanism is still under discussion since no evidence of cell cycle arrest was found, but an antioxidant role was shown for some of the selective complexes. All complexes were also tested as antimicrobial drugs, exhibiting good activity towards S. aureus and E. coli. bacteria and C. albicans and C. neoformans fungi.
Vaccinia Virus: From Crude Smallpox Vaccines to Elaborate Viral Vector Vaccine Design
Authors: Kaynarcalidan Onur , Moreno Mascaraque Sara , Drexler Ingo
Published: November 2021

Various vaccinia virus (VACV) strains were applied during the smallpox vaccination campaign to eradicate the variola virus worldwide. After the eradication of smallpox, VACV gained popularity as a viral vector thanks to increasing innovations in genetic engineering and vaccine technology. Some VACV strains have been extensively used to develop vaccine candidates against various diseases. Modified vaccinia virus Ankara (MVA) is a VACV vaccine strain that offers several advantages for the development of recombinant vaccine candidates. In addition to various host-restriction genes, MVA lacks several immunomodulatory genes of which some have proven to be quite efficient in skewing the immune response in an unfavorable way to control infection in the host. Studies to manipulate these genes aim to optimize the immunogenicity and safety of MVA-based viral vector vaccine candidates. Here we summarize the history and further work with VACV as a vaccine and present in detail the genetic manipulations within the MVA genome to improve its immunogenicity and safety as a viral vector vaccine.
A Customized Microfluidic Paper-Based Platform for Colorimetric Immunosensing: Demonstrated via hCG Assay for Pregnancy Test
Authors: Rahbar Mohammad , Zou Siyi , Baharfar Mahroo , Liu Guozhen
Published: November 2021

Over the past decades, paper-based lateral flow immunoassays (LFIAs) have been extensively developed for rapid, facile, and low-cost detection of a wide array of target analytes in a point-of-care manner. Conventional home pregnancy tests are the most significant example of LFAs, which detect elevated concentrations of human chorionic gonadotrophin (hCG) in body fluids to identify early pregnancy. In this work, we have upgraded these platforms to a higher version by developing a customized microfluidic paper-based analytical device (μPAD), as the new generation of paper-based point-of-care platforms, for colorimetric immunosensing. This will offer a cost-efficient and environmentally friendly alternative platform for paper-based immunosensing, eliminating the need for nitrocellulose (NC) membrane as the substrate material. The performance of the developed platform is demonstrated by detection of hCG (as a model case) in urine samples and subsequently indicating positive or negative pregnancy. A dual-functional silane-based composite was used to treat filter paper in order to enhance the colorimetric signal intensity in the detection zones of μPADs. In addition, microfluidic pathways were designed in a manner to provide the desired regulated fluid flow, generating sufficient incubation time (delays) at the designated detection zones, and consequently enhancing the obtained signal intensity. The presented approaches allow to overcome the existing limitations of μPADs in immunosensing and will broaden their applicability to a wider range of assays. Although, the application of the developed hCG μPAD assay is mainly in qualitative (i.e., positive or negative) detection of pregnancy, the semi-quantitative measurement of hCG was also investigated, indicating the viability of this assay for sensitive detection of the target hCG analyte within the related physiological range (i.e., 10–500 ng/mL) with a LOD value down to 10 ng/mL.
Exhaled Breath Analysis for Diabetes Diagnosis and Monitoring: Relevance, Challenges and Possibilities
Authors: Dixit Kaushiki , Fardindoost Somayeh , Ravishankara Adithya , Tasnim Nishat , Hoorfar Mina
Published: November 2021

With the global population prevalence of diabetes surpassing 463 million cases in 2019 and diabetes leading to millions of deaths each year, there is a critical need for feasible, rapid, and non-invasive methodologies for continuous blood glucose monitoring in contrast to the current procedures that are either invasive, complicated, or expensive. Breath analysis is a viable methodology for non-invasive diabetes management owing to its potential for multiple disease diagnoses, the nominal requirement of sample processing, and immense sample accessibility; however, the development of functional commercial sensors is challenging due to the low concentration of volatile organic compounds (VOCs) present in exhaled breath and the confounding factors influencing the exhaled breath profile. Given the complexity of the topic and the skyrocketing spread of diabetes, a multifarious review of exhaled breath analysis for diabetes monitoring is essential to track the technological progress in the field and comprehend the obstacles in developing a breath analysis-based diabetes management system. In this review, we consolidate the relevance of exhaled breath analysis through a critical assessment of current technologies and recent advancements in sensing methods to address the shortcomings associated with blood glucose monitoring. We provide a detailed assessment of the intricacies involved in the development of non-invasive diabetes monitoring devices. In addition, we spotlight the need to consider breath biomarker clusters as opposed to standalone biomarkers for the clinical applicability of exhaled breath monitoring. We present potential VOC clusters suitable for diabetes management and highlight the recent buildout of breath sensing methodologies, focusing on novel sensing materials and transduction mechanisms. Finally, we portray a multifaceted comparison of exhaled breath analysis for diabetes monitoring and highlight remaining challenges on the path to realizing breath analysis as a non-invasive healthcare approach.
Ten Years Progress of Electrical Detection of Heavy Metal Ions (HMIs) Using Various Field-Effect Transistor (FET) Nanosensors: A Review
Authors: Falina Shaili , Syamsul Mohd , Rhaffor Nuha Abd , Sal Hamid Sofiyah , Mohamed Zain Khairu Anuar , Abd Manaf Asrulnizam , Kawarada Hiroshi
Published: November 2021

Heavy metal pollution remains a major concern for the public today, in line with the growing population and global industrialization. Heavy metal ion (HMI) is a threat to human and environmental safety, even at low concentrations, thus rapid and continuous HMI monitoring is essential. Among the sensors available for HMI detection, the field-effect transistor (FET) sensor demonstrates promising potential for fast and real-time detection. The aim of this review is to provide a condensed overview of the contribution of certain semiconductor substrates in the development of chemical and biosensor FETs for HMI detection in the past decade. A brief introduction of the FET sensor along with its construction and configuration is presented in the first part of this review. Subsequently, the FET sensor deployment issue and FET intrinsic limitation screening effect are also discussed, and the solutions to overcome these shortcomings are summarized. Later, we summarize the strategies for HMIs’ electrical detection, mechanisms, and sensing performance on nanomaterial semiconductor FET transducers, including silicon, carbon nanotubes, graphene, AlGaN/GaN, transition metal dichalcogenides (TMD), black phosphorus, organic and inorganic semiconductor. Finally, concerns and suggestions regarding detection in the real samples using FET sensors are highlighted in the conclusion.
Multiplexed, High-Sensitivity Measurements of Antibody Affinity Using Interferometric Reflectance Imaging Sensor
Authors: Marn Allison M. , Needham James , Chiodi Elisa , Ünlü M. Selim
Published: November 2021

Anthrax lethal factor (LF) is one of the enzymatic components of the anthrax toxin responsible for the pathogenic responses of the anthrax disease. The ability to screen multiplexed ligands against LF and subsequently estimate the effective kinetic rates (kon and koff) and complementary binding behavior provides critical information useful in diagnostic and therapeutic development for anthrax. Tools such as biolayer interferometry (BLI) and surface plasmon resonance imaging (SPRi) have been developed for this purpose; however, these tools suffer from limitations such as signal jumps when the solution in the chamber is switched or low sensitivity. Here, we present multiplexed antibody affinity measurements obtained by the interferometric reflectance imaging sensor (IRIS), a highly sensitive, label-free optical biosensor, whose stability, simplicity, and imaging modality overcomes many of the limitations of other multiplexed methods. We compare the multiplexed binding results obtained with the IRIS system using two ligands targeting the anthrax lethal factor (LF) against previously published results obtained with more traditional surface plasmon resonance (SPR), which showed consistent results, as well as kinetic information previously unattainable with SPR. Additional exemplary data demonstrating multiplexed binding and the corresponding complementary binding to sequentially injected ligands provides an additional layer of information immediately useful to the researcher.
Integration of Smart Pavement Data with Decision Support Systems: A Systematic Review
Authors: Amândio Margarida , Parente Manuel , Neves José , Fonseca Paulo
Published: November 2021

Nowadays, pavement management systems (PMS) are mainly based on monitoring processes that have been established for a long time, and strongly depend on acquired experience. However, with the emergence of smart technologies, such as internet of things and artificial intelligence, PMS could be improved by applying these new smart technologies to their decision support systems, not just by updating their data collection methodologies, but also their data analysis tools. The application of these smart technologies to the field of pavement monitoring and condition evaluation will undoubtedly contribute to more efficient, less costly, safer, and environmentally friendly methodologies. Thus, the main drive of the present work is to provide insight for the development of future decision support systems for smart pavement management by conducting a systematic literature review of the developed works that apply smart technologies to this field. The conclusions drawn from the analysis allowed for the identification of a series of future direction recommendations for researchers. In fact, future PMS should tend to be capable of collecting and analyzing data at different levels, both externally at the surface or inside the pavement, as well as to detect and predict all types of functional and structural flaws and defects.
Thermal Performance Assessment of a Wall Made of Lightweight Concrete Blocks with Recycled Brick and Ground Polystyrene
Authors: Krstić Hrvoje , Miličević Ivana , Markulak Damir , Domazetović Mihaela
Published: November 2021

Hollow concrete masonry blocks made of low strength self-compacting concrete with recycled crushed brick and ground polystyrene as an aggregate (RBC-EP blocks), and their expected structural role as masonry infill in steel frames, has been confirmed in previous research studies, thus the extensive investigation of thermal properties is presented in this paper to fully approve their potential application in practice. The Heat Flow and Temperature Based Method was used to conduct in-situ measurements of the wall thermal transmittance (U-value). The experimental U-values of the wall without insulation varied from 1.363 to 1.782 W/m2·K, and the theoretical value was calculated to be 2.01 W/m2·K. Thermal conductivity of the material used for making RBC-EP blocks was measured in a laboratory by using a heat flow meter instrument. To better understand the thermal performance characteristics of a wall constructed from RBC-EP blocks, a comparison with standard materials currently used and found on the market was performed. Walls constructed from RBC-EP blocks show an improvement of building technology and environmentally based enhancement of concrete blocks, since they use recycled materials. They can replace standard lightweight concrete blocks due to their desired mechanical properties, as well as the better thermal performance properties compared to commonly used materials for building walls.
Commercial Buildings in Town. The Influence of Discount Shops on the Trade Structure of a Small Town
Authors: Szumilas Agnieszka , Pach Pawel
Published: November 2021

Changes taking place in the spatial structure of trade in cities have been very dynamic in recent decades. The goal of the research was to determine the dynamics and direction of changes in shaping the traditional commercial structure of small towns, in the context of discount shops location. The subject matter of the research constituted more than ten towns in southern Poland. Traditional shops located there were analysed in terms of their location, the profile and length of their activity. Studies on global trade development processes are available, as well as detailed research results on individual trade assumptions. On the other hand, there is a research gap regarding studies involving the location of commercial buildings, especially in small towns. The research results are a valuable source of information for representatives of various scientific disciplines as well as city authorities. The presented research provides knowledge and constitutes the basis for further research for representatives of various research fields. The areas with the most intensive commercial activity in the last 30 years were correlated with the number of inhabitants. For a selected group of towns, their binding urban planning documentation was verified with respect to specifications facilitating or excluding the establishment of discount shops. The conclusions reached on the basis of the research do not confirm a popular view that discount shops have a negative effect on urban space. The performed analyses indicate that there is no simple relation between a discount shop location and the decreasing number of traditional shops in the investigated cities. The presented research results gain an additional advantage—they form an archive of the development of commerce structure in towns in the period starting with the fall of the centrally planned economy and finishing with the COVID–19 pandemic. The presented material forms a basis for further urban studies which will use the experience gained at this stage
Effects of Wall-to-Wall Supported Ceilings on Impact Sound Insulation for Use in Residential Buildings
Authors: Kim Sin-Tae , Cho Hyun-Min , Kim Myung-Jun
Published: November 2021

In Korean residential buildings, floor impact sounds were reduced over the past few decades mainly through a floating floor system. However, ceiling constructions for impact sound reduction have not been applied actively because of a lack of useful information. This study focuses on the effects of wall-to-wall supported ceilings (WSC), which are designed with construction discontinuities between concrete slabs and ceilings, and the damping caused by porous absorbers for impact sound insulation. To examine the impact sound insulation according to ceiling conditions, measurements were performed in 25 floor–ceiling assemblies. The results indicate that ceiling treatment is mostly useful in reducing the floor impact sound. The floor impact sound owing to the WSC decreased by 2–7 dB and 2–8 dB in terms of the single number quantity for the tapping machine and rubber balls, respectively, compared with representative existing housing constructions wherein ceilings were attached on wooden sticks. Furthermore, the reduction effect of the WSC appeared to be more profound when it was applied to the floor–ceiling assembly with poor impact sound insulation. Thus, the WSC can be used to enhance the impact of sound insulation of existing housings without major repairs of floor structural layers.
Interaction between Thermal Comfort, Indoor Air Quality and Ventilation Energy Consumption of Educational Buildings: A Comprehensive Review
Authors: Jia Lin-Rui , Han Jie , Chen Xi , Li Qing-Yun , Lee Chi-Chung , Fung Yat-Hei
Published: November 2021

Thermal comfort and indoor air quality (IAQ) of educational buildings can affect students’ academic performance and well-being and are closely related to ventilation energy consumption. Demands of the indoor environmental quality within the classroom generally vary with the education levels and result in ventilation energy consumption accounting for a considerable proportion of the total energy use in bulk educational buildings. Its huge energy-saving potential is attracting worldwide attention from scholars and governments. Therefore, appropriate operation strategies of ventilation systems should be adopted to effectively reduce energy consumption without sacrificing thermal comfort and IAQ. However, the absence of relevant standards and guidelines for designing a quality classroom environment considering the special features of educational buildings remains an important research question. This study conducts a comprehensive review to determine research gaps and identify future directions for the interaction between thermal comfort, IAQ and ventilation energy consumption for educational buildings. The review results show that: (1) The thermal comfort prediction model should consider the influences of genders, ages and socioeconomic backgrounds; (2) The mixed-mode ventilation coupling the natural and mechanical approaches is preferred given its advantage of lower energy consumption and improved thermal comfort, but its control strategies need further exploration; (3) Optimizing passive design parameters of buildings (e.g., window to wall ratios, window orientations and sun shading installations) can significantly reduce the ventilation demands while maintaining indoor thermal comfort; (4) More studies are required for investigating thermal comfort in educational buildings during the heating period; and (5) IAQ of university buildings clearly requires further studies, especially on bacterial and fungal aerosol pollutants, for a more comprehensive assessment of the built environment.
Derivation of Kinematic Equations Based on Full-Scale Racking Tests for Seismic Performance Evaluation of Unitized Four-Sided Structural Sealant Glazing Curtain Wall Systems
Authors: Memari Ali M. , Simmons Nicholas , Solnosky Ryan L.
Published: November 2021

Curtain wall glazing systems are a major part of a building due to the multiple roles they have, including occupant protection against environmental effects and the transfer of loads to the structural system. From a structural perspective, limited analytical guidelines and methods exist to aid designers in their determination of the curtain wall performance without extensive simulation or laboratory testing. This study takes experimental data from full-scale, “unitized”, four-sided structural sealant glazing (4SSG) curtain wall system mockups featuring a re-entrant corner subjected to cyclic racking displacements in accordance with the American Architectural Manufacturers Association AAMA 501.6 protocol to derive and establish equations that predict the relative displacements of the glass relative to the glazing frame, based on the amount of inter-story drift. Through derivation and testing, sealant cohesive failure and glass cracking were identified as limit states and corresponding drift levels were determined to control many of the equations. Displacements from the newly derived equations were correlated to the effective shear strain value experienced by the structural silicone in the mockup concurrently with the curtain wall’s drift capacity. This paper provides detailed derivation of the kinematic equations for possible use by glazing design professionals. Such equations can help designers to more easily predict the drifts that cause damage to such systems by manual calculations without the need for expensive mockup testing or time-intensive computer models.
It’s Time to Shift the Paradigm: Translation and Clinical Application of Non-αvβ3 Integrin Targeting Radiopharmaceuticals
Authors: Kossatz Susanne , Beer Ambros Johannes , Notni Johannes
Published: November 2021

For almost the entire period of the last two decades, translational research in the area of integrin-targeting radiopharmaceuticals was strongly focused on the subtype αvβ3, owing to its expression on endothelial cells and its well-established role as a biomarker for, and promoter of, angiogenesis. Despite a large number of translated tracers and clinical studies, a clinical value of αvβ3-integrin imaging could not be defined yet. The focus of research has, thus, been moving slowly but steadily towards other integrin subtypes which are involved in a large variety of tumorigenic pathways. Peptidic and non-peptidic radioligands for the integrins α5β1, αvβ6, αvβ8, α6β1, α6β4, α3β1, α4β1, and αMβ2 were first synthesized and characterized preclinically. Some of these compounds, targeting the subtypes αvβ6, αvβ8, and α6β1/β4, were subsequently translated into humans during the last few years. αvβ6-Integrin has arguably attracted most attention because it is expressed by some of the cancers with the worst prognosis (above all, pancreatic ductal adenocarcinoma), which substantiates a clinical need for the respective theranostic agents. The receptor furthermore represents a biomarker for malignancy and invasiveness of carcinomas, as well as for fibrotic diseases, such as idiopathic pulmonary fibrosis (IPF), and probably even for Sars-CoV-2 (COVID-19) related syndromes. Accordingly, the largest number of recent first-in-human applications has been reported for radiolabeled compounds targeting αvβ6-integrin. The results indicate a substantial clinical value, which might lead to a paradigm change and trigger the replacement of αvβ3 by αvβ6 as the most popular integrin in theranostics.
Hypoxia and Its Influence on Radiotherapy Response of HPV-Positive and HPV-Negative Head and Neck Cancer
Authors: Wegge Marilyn , Dok Rüveyda , Nuyts Sandra
Published: November 2021

Head and neck squamous cancers are a heterogeneous group of cancers that arise from the upper aerodigestive tract. Etiologically, these tumors are linked to alcohol/tobacco abuse and infections with high-risk human papillomavirus (HPV). HPV-positive HNSCCs are characterized by a different biology and also demonstrate better therapy response and survival compared to alcohol/tobacco-related HNSCCs. Despite this advantageous therapy response and the clear biological differences, all locally advanced HNSCCs are treated with the same chemo-radiotherapy schedules. Although we have a better understanding of the biology of both groups of HNSCC, the biological factors associated with the increased radiotherapy response are still unclear. Hypoxia, i.e., low oxygen levels because of an imbalance between oxygen demand and supply, is an important biological factor associated with radiotherapy response and has been linked with HPV infections. In this review, we discuss the effects of hypoxia on radiotherapy response, on the tumor biology, and the tumor microenvironment of HPV-positive and HPV-negative HNSCCs by pointing out the differences between these two tumor types. In addition, we provide an overview of the current strategies to detect and target hypoxia.
Immune Checkpoints and Innate Lymphoid Cells—New Avenues for Cancer Immunotherapy
Authors: Jacquelot Nicolas , Ghaedi Maryam , Warner Kathrin , Chung Douglas C. , Crome Sarah Q. , Ohashi Pamela S.
Published: November 2021

Immune checkpoints (IC) are broadly characterized as inhibitory pathways that tightly regulate the activation of the immune system. These molecular “brakes” are centrally involved in the maintenance of immune self-tolerance and represent a key mechanism in avoiding autoimmunity and tissue destruction. Antibody-based therapies target these inhibitory molecules on T cells to improve their cytotoxic function, with unprecedented clinical efficacies for a number of malignancies. Many of these ICs are also expressed on innate lymphoid cells (ILC), drawing interest from the field to understand their function, impact for anti-tumor immunity and potential for immunotherapy. In this review, we highlight ILC specificities at different tissue sites and their migration potential upon inflammatory challenge. We further summarize the current understanding of IC molecules on ILC and discuss potential strategies for ILC modulation as part of a greater anti-cancer armamentarium.
Updated Principles of Surgical Management of Pancreatic Neuroendocrine Tumours (pNETs): What Every Surgeon Needs to Know
Authors: de Ponthaud Charles , Menegaux Fabrice , Gaujoux Sébastien
Published: November 2021

Pancreatic neuroendocrine tumours (pNETs) represent 1 to 2% of all pancreatic neoplasm with an increasing incidence. They have a varied clinical, biological and radiological presentation, depending on whether they are sporadic or genetic in origin, whether they are functional or non-functional, and whether there is a single or multiple lesions. These pNETs are often diagnosed at an advanced stage with locoregional lymph nodes invasion or distant metastases. In most cases, the gold standard curative treatment is surgical resection of the pancreatic tumour, but the postoperative complications and functional consequences are not negligible. Thus, these patients should be managed in specialised high-volume centres with multidisciplinary discussion involving surgeons, oncologists, radiologists and pathologists. Innovative managements such as “watch and wait” strategies, parenchymal sparing surgery and minimally invasive approach are emerging. The correct use of all these therapeutic options requires a good selection of patients but also a constant update of knowledge. The aim of this work is to update the surgical management of pNETs and to highlight key elements in view of the recent literature.
NiO-TiO2 p-n Heterojunction for Solar Hydrogen Generation
Authors: Zheng Dewen , Zhao Heng , Wang Shanyu , Hu Jinguang , Chen Zhangxin
Published: November 2021

Photocatalytic water splitting for hydrogen production has been widely recognized as a promising strategy for relieving the pressure from energy crisis and environmental pollution. However, current efficiency for photocatalytic hydrogen generation has been limited due to a low separation of photogenerated electrons and holes. p-n heterojunction with a built-in electric field emerges as an efficient strategy for photocatalyst design to boost hydrogen evolution activities due to a spontaneous charge separation. In this work, we investigated the effect of different preparation methods on photocatalytic hydrogen production over NiO-TiO2 composites. The results demonstrated that a uniform distribution of NiO on a surface of TiO2 with an intimate interfacial interaction was formed by a sol-gel method, while direct calcination tended to form aggregation of NiO, thus leading to an uneven p-n heterojunction structure within a photocatalyst. NiO-TiO2 composites fabricated by different methods showed enhanced hydrogen production (23.5 ± 1.2, 20.4 ± 1.0 and 8.8 ± 0.7 mmolh−1g−1 for S1-20%, S2-20% and S3-10%, respectively) as compared with pristine TiO2 (6.6 ± 0.7 mmolh−1g−1) and NiO (2.1 ± 0.2 mmolh−1g−1). The current work demonstrates a good example to improve photocatalytic hydrogen production by finely designing p-n heterojunction photocatalysts.
Plasma Promotes Dry Reforming Reaction of CH4 and CO2 at Room Temperature with Highly Dispersed NiO/γ-Al2O3 Catalyst
Authors: Lin Shan-Shan , Li Peng-Rui , Jiang Hui-Bo , Diao Jian-Feng , Xu Zhong-Ning , Guo Guo-Cong
Published: November 2021

Plasma is an efficient method that can activate inert molecules such as methane and carbon dioxide in a mild environment to make them reactive. In this work, we have prepared an AE-NiO/γ-Al2O3 catalyst using an ammonia-evaporation method for plasma promoted dry reforming reaction of CO2 and CH4 at room temperature. According to the characterization data of XRD, H2-TPR, TEM, XPS, etc., the AE-NiO/γ-Al2O3 catalyst has higher dispersion, smaller particle size and stronger metal-support interaction than the catalyst prepared by the traditional impregnation method. In addition, the AE-NiO/γ-Al2O3 catalyst also exhibits higher activity in dry reforming reaction. This work provides a feasible reference experience for the research of plasma promoted dry reforming reaction catalysts at room temperature.
Advancements in Solar Desalination of Seawater by Various Ti3C2 MXene Based Morphologies for Freshwater Generation: A Review
Authors: Sreedhar Adem , Noh Jin-Seo
Published: November 2021

For a few years, we have been witnessing ubiquitous fresh and drinking water scarcity in various countries. To mitigate these problematic situations, many countries relied on non-conventional freshwater generation technologies through solar desalination of seawater. In this manner, we excel the ability of new class 2D Ti3C2 MXenes as a photothermal material (solar absorber) for freshwater generation via the solar desalination technique. In this review, the air–water interfacial interaction is highlighted for improving the evaporation efficiency. To provide the dependence of the desalination efficiency on the microstructure of the solar absorbers, we summarized various forms of 2D Ti3C2 MXenes (aerosol, films, foam, hydrogel, membrane, monolith and porous structure) and their characteristics. These microstructures prevailed ultrahigh photoconversion efficiency. In this aspect, we further explained key features such as light absorption, reflection, multiple internal reflection, hydrophilicity, lower thermal conduction, light-to-heat generation, and salt rejection for achieving efficient desalination output throughout the visible and broadband region. Specifically, we targeted to explore the self-floating and salt rejection nature of various state-of-the-art 2D Ti3C2 MXene structures. Further, we highlighted the long-term stability. Among the above morphologies, Ti3C2 MXene in the form of a membrane is believed to be a promising morphology which effectively desalinates seawater into freshwater. Finally, we highlighted the challenges and future perspectives, which can pave a potential path for advancing the sustainable solar desalination of seawater into freshwater.
Gold(I)-Catalyzed Tandem Synthesis of Polycyclic Dihydroquinazolinones
Authors: Sun Jingyang , Song Yoona , Ryu Jae-Sang
Published: November 2021

A gold-catalyzed cascade process for the synthesis of dihydroquinazolinone scaffolds was developed. A series of gold catalysts were screened for this tandem transformation, and the (PPh3)AuCl/AgOTf catalyst combination was found to be the best catalyst system. This method is characterized by good yields, high regioselectivity, and broad substrate scope. This method is also applicable to the synthesis of tetracyclic dihydroquinazolinones and seven-membered ring-fused dihydroquinazolinones.