bluuebunny/crossref_metadata_2025 huggingface dataset:
Relationship Between Occupants’ Adaptive Behaviors, Air-Conditioning Usage, and Thermal Acceptability Among Residences in the Hot–Humid Climate of Indonesia
Authors: Apriliyanthi Sri Rahma , Sakoi Tomonori , Kubota Tetsu , Nakaya Takashi , Koerniawan Mochammad Donny , Alfata Muhammad Nur Fajri , Aziiz Akhlish Diinal , Suhedi Fefen , Fathuna Inat Shani , Takiguchi Taiga
Published: January 2025

A strategy for effectively utilizing occupants’ adaptive behaviors (OABs) to achieve thermal acceptability while maintaining low energy consumption is necessary. This study aims to clarify the relationship between OABs and thermal acceptability over various climate zones, as well as the change in OABs due to air conditioner (AC) ownership in Indonesian residences. An online questionnaire consisting of perceived OABs’ time intensity, thermal acceptability, and personal attributes from 3000 respondents across Indonesia was analyzed using logistic regression. The results suggested that NV occupants engage in more fan usage and window opening to enhance ventilative cooling, while AC occupants are more likely to adjust clothing and use portable fans to create cooler environments. Moreover, the effects of OABs on NV residences varied depending on the local climate conditions. In hot local climates, averages of 90% fan usage intensity and 92% window opening intensity, complemented with active clothing adjustment, were unable to effectively provide thermal acceptability. These findings imply that there is a range of indoor environmental conditions in which conventional OABs work well. This study highlights the need to promote behavioral adaptations, especially in AC mixed-mode buildings, and to consider behavioral adaptations in NV buildings based on local climates.
Economic and Carbon Emission Analyses of C50 Manufactured Sand Concrete Considering Workability and Compressive Strength
Authors: Li Ning , Zhang Zewei , Hu Dongxia , Pang Guangwei , Wang Qian , Si Wei
Published: January 2025

C50 manufactured sand concrete requires good workability and strength, and economic efficiency and carbon emissions also need to be considered. This study incorporates sensitivity and significance analyses to recommend the optimal economic mix composition for C50 manufactured sand concrete. The relationship between cost, workability, and mechanical properties was analyzed by considering the water/binder ratio, sand ratio, fly ash content, and superplasticizer dosage. An optimal composition of C50 manufactured sand concrete was recommended. The cost and carbon emissions were quantified at the optimal composition. The results showed that the water/binder ratio had the most significant impact on the cost and carbon emission, while the sand ratio and superplasticizer dosage had the least. All factors significantly affected its cost and carbon emission. Compared to natural sand concrete, manufactured sand concrete achieved a lower cost but higher carbon emissions. Considering the workability, strength, and cost per cubic meter of concrete, the most economical mix proportion for C50 concrete was recommended with a water/binder ratio of 0.36, a fly ash content of 25%, a sand ratio of 0.42, and a superplasticizer dosage of 1.2%. This composition cost 356 yuan, and carbon emission was 352.6 kg CO2 per cubic meter of concrete. Compared to a composition with a water/binder ratio of 0.34 and fly ash content of 15%, the unit cost can be reduced by 18.4 yuan, and carbon emission can be minimized by 56.6 kg CO2 e/m3. The appropriate water/binder ratio and fly ash content can reduce cost and carbon emissions without compromising the workability, compressive strength, or elastic modulus of C50 concrete. This achieves triple benefits in terms of performance, economy, and the environment when applying C50 manufactured sand concrete.
Effects of Retrofit Strategies on Thermal Comfort and Energy Performance in Social Housing for Current and Future Weather Scenarios
Authors: Basaly Lucienne G. , Hashemi Arman , Elsharkawy Heba , Newport Darryl , Badawy Nancy Mahmoud
Published: January 2025

With growing concerns over energy and heat-related mortality/morbidity rates, enhancing building performances is key to improving the health and well-being of building occupants while reducing CO2 emissions, in line with the UK Government’s Net-Zero targets. This study investigates the impacts of different retrofitting scenarios on overheating risk and energy performance in social housing for current and future climate conditions. Dynamic thermal simulations were carried out using Design Summer Year (DSY) weather files in DesignBuilder software for selected case study buildings. Winter performance was analysed using the Predicted Mean Vote (PMV) index, while summer results were assessed according to the Chartered Institution of Building Services Engineers Technical Memorandum 59 (CIBSE TM59) guidelines. The findings revealed that bedrooms, especially those facing south, were at high risk of overheating. Factors such as building construction, the number of exposed surfaces, and window area influenced the risks. External wall insulation outperformed internal wall insulation in improving summer comfort. In the winter, Passivhaus standards with natural ventilation ensured thermal comfort across all zones, with a 41–53% reduction in heating energy consumption under current weather conditions. The risk of overheating and associated health issues significantly increased for the future weather scenarios. Further investigation into ventilation strategies, occupant behaviour, and passive design is required to mitigate overheating risks while reducing energy consumption in buildings.
The Domestic Acoustic Environment in Online Education—Part 1: Differences by Gender, Perceived Academic Quality, and Self-Rated Performance
Authors: Puyana-Romero Virginia , Díaz-Márquez Angela María , Garzón Christiam , Ciaburro Giuseppe
Published: January 2025

The domestic environment plays a critical role in online education, particularly its acoustic aspect. This study investigates whether there are differences in the perception of acoustic variables between students who perceive their academic performance as negative (G1) and those who rate it positively (G2). It also explores differences between students who assess the quality of education as positive or negative, along with gender distinctions. Data were collected through an online survey conducted by Universidad de Las Américas, Quito, Ecuador, during the COVID-19 lockdown. Statistical analyses revealed significant differences in the perception of noise between groups G1 and G2: Students who rated their academic performance negatively experienced higher noise interference from sources like voices and household appliances. Similar patterns were observed when assessing educational quality. Gender differences also emerged, with women reporting higher levels of noise interference caused by sound sources (voices, TV/Radio/household appliances, and animals), and in the development of autonomous and synchronous tasks than men. The differences between the groups evaluated in pairs suggest that these aspects should be taken into account when designing domestic spaces and strategies to improve the quality of the acoustic environment for students.
Occupants’ Thermal Adaptive Behavior Pattern in Indonesian Residential Buildings
Authors: Apriliyanthi Sri Rahma , Sakoi Tomonori , Nakaya Takashi , Kubota Tetsu , Koerniawan Mochammad Donny , Alfata Muhammad Nur Fajri , Aziiz Akhlish Diinal , Suhedi Fefen , Fathuna Inat Shani
Published: January 2025

Occupants’ adaptive behaviors (OABs) can boost thermal acceptability. This study aims to identify the OAB pattern in the hot-humid region of Indonesia and compare the thermal acceptability percentage of the groups with the identified patterns. Data from an online questionnaire with 3000 respondents were adopted for the analysis of air conditioning (AC), fan, and portable fan usage intensities, along with AC set-point temperature, clo-value, clothing adjustment, and window opening intensity. Hierarchical cluster analysis and logistic regressions were used to distinguish and evaluate OAB patterns. Five groups with various patterns for each AC and naturally ventilated (NV) residence were identified, and similar pattern groups with high clo-values and low thermal acceptability percentages appeared in both residences. The highest window opening intensity for the most active patterns in both AC and NV residences was perceived to be 87% and 91% as time intensity averages. These findings imply a great influence of NV lifestyles with active behavior patterns on improving thermal acceptability for both residences. This study suggests an appropriate OAB strategy to promote a better trade-off between energy consumption and thermal acceptability, such as patterns with a ±25 °C set-point temperature and various OABs in AC residences. Additionally, further investigation into the socio-cultural effect of OABs is necessary.
Effect of Stiffeners on the Axial Strength of Face-to-Face Unequal-Angle Cold-Formed Steel Columns
Authors: Gurupatham Beulah Gnana Ananthi , Roy Krishanu , Lim James Boon Piang
Published: January 2025

Cold-formed steel (CFS) unsymmetrical angles are increasingly used in structural applications such as portal frames, roof trusses, and transmission towers. However, research on built-up face-to-face unsymmetrical CFS angle columns (FFUACs) with stiffeners remains limited. This study addresses this gap by presenting the findings from six experimental investigations on intermediate FFUACs connected using intermittent screw fasteners. The results offer insights into failure deformation patterns and load-axial shortening behaviour. A nonlinear finite element (FE) model was developed to account for material and geometric nonlinearity, with experimental results used for validation. This study contributes 166 new data points, including six experimental tests under concentric compression and 160 finite element analysis (FEA) results focused on the compressive strength of FFUACs. Additionally, this study evaluates the performance of existing design guidelines based on the direct strength method (DSM). The DSM strength predictions were found to be less conservative for stub FFUAC specimens that failed due to local buckling and more conservative for short FFUAC specimens that failed due to a combination of local and flexural buckling. A revised DSM methodology is proposed to address these discrepancies.
Everyday-Carry Equipment Mapping: A Portable and Low-Cost Method for 3D Digital Documentation of Architectural Heritage by Integrated iPhone and Microdrone
Authors: Zhang Nan , Lan Xijian
Published: January 2025

Mapping constitutes a critical component of architectural heritage research, providing the groundwork for both conservation and utilization efforts. Three-dimensional (3D) digital documentation represents a prominent form of mapping in the contemporary era, and its value is widely recognized. However, cost and portability constraints often limit its widespread use in routine research and conservation initiatives. This study proposes a cost-effective and portable approach to 3D digital documentation, employing everyday-carry (EDC) equipment, the iPhone 15 Pro and DJI Mini 4 Pro, for data acquisition in architectural heritage. The workflow was subsequently optimized, and the datasets from the iPhone-LiDAR and microdrone were seamlessly integrated, resulting in an integrated 3D digital model of both the indoor and outdoor spaces of the architectural heritage site. The model demonstrated an overall relative error of 4.93%, achieving centimeter-level accuracy, precise spatial alignment between indoor and outdoor sections, clear and smooth texture mapping, high visibility, and suitability for digital display applications. This optimized workflow leverages the strengths of both EDC equipment types while addressing the limitations identified in prior studies.
Design of Prestressed Cable Dome Using Minor Perturbation Method
Authors: Zhou Haitao , Fu Feng , Si Bo , You Deqing , Zhang Fengjian
Published: January 2025

For the structural design of cable domes, the determination of prestress force distribution, the section of the structural components, and initial configuration are prerequisites for the subsequent detailed design of cable and strut sizes. To solve this problem, this paper elucidates the basic theory of the Minor Perturbation Method, introduces this theory into the field of force finding design for cable dome structures, and develops a new design method whose core is the comparison between the combined stress of each component conforming to mechanical characteristics of cable-strut structure and control stress, and meeting the convergence condition by adjusting the prestress level and cross-section of components. A corresponding design flow chart is established and programmed with finite element analysis software. Through the case studies of two different kinds of cable dome, it is proven that the proposed method and software program can simply, quickly, and effectively design the cable domes with an economic cross-section.
Numerical Study on the Risk of Infection in Adjacent Residential Spaces: Door Operation and the Impact of Outdoor Wind Speeds
Authors: Wu Xunmei , Han Mengtao , Chen Hong
Published: January 2025

Infectious diseases have profoundly impacted global health and daily life. To control virus transmission, countries worldwide have implemented various preventive measures. A critical pathway for infection spread is cross-infection within households, especially among family members in the same or adjacent rooms. This study uses numerical simulations to examine aerosol transmission characteristics in adjacent spaces in home settings and assess associated infection risks. The study evaluated the effects of factors such as outdoor wind speed, door gap leakage, and door opening actions on aerosol concentration and infection risk across various areas. Key conclusions include the following: Under prolonged lack of ventilation, aerosol leakage through the door gap is minimal, with the average aerosol concentration outside the bedroom remaining low (<0.04). In the absence of ventilation, aerosol accumulation primarily occurs within the bedroom. Under ventilated conditions, door gap leakage may increase infection risk in adjacent areas, suggesting a stay duration of no more than 75 min to keep infection risk below 30%. The findings provide practical recommendations for airtight design and activity area selection within residential spaces, offering valuable guidance for effective infection control measures.
Workplace Well-Being Through Nature-Based Solutions: A Fuzzy Framework for Decision-Making
Authors: Bressane Adriano , de Castro Marcos Vinícius
Published: January 2025

The rise in performance and health concerns presents a complex challenge to workplace well-being. While existing research highlights the positive impact of exposure to nature on well-being, there is a gap in supporting biophilic building designs in workplaces. This study addresses this issue by introducing a Type-1 Interval Fuzzy Set (IFS) framework to assess workplace health, and recommends Nature-Based Solutions (NbS) for enhancement. The proposed framework comprises two fuzzy subsystems targeting environmental comfort and occupational health that are integrated into a third subsystem to provide tailored NbS recommendations. A case study conducted in a coworking space validated the framework’s practical application, revealing significant improvements in environmental and occupational conditions. Key findings include a reduction in absenteeism, enhancement in employee satisfaction, and creation of more comfortable and restorative environments. Recommendations, such as green walls, indoor gardens, and natural light filters, have been shown to effectively mitigate discomfort and promote well-being. The framework demonstrated flexibility in managing uncertainties, optimizing resource allocation, and providing targeted interventions for workplace conditions. These results highlight the framework’s potential as a robust decision-support tool for improving workplace health and well-being through systematic and evidence-based strategies.
Tumor Cell Survival Factors and Angiogenesis in Chronic Lymphocytic Leukemia: How Hot Is the Link?
Authors: Ayoub Marianne , Susin Santos A. , Bauvois Brigitte
Published: January 2025

Chronic lymphocytic leukemia (CLL) is characterized by the accumulation of neoplastic CD5+/CD19+ B lymphocytes in the blood. These cells migrate to and proliferate in the bone marrow and lymphoid tissues. Despite the development of new therapies for CLL, drug resistance and disease relapse still occur; novel treatment approaches are therefore still needed. Inhibition of the angiogenesis involved in the progression of CLL might be a relevant therapeutic strategy. The literature data indicate that vascular endothelial growth factor, angiopoietin-2, and matrix metalloproteinase-9 are pro-angiogenic factors in CLL. A number of other CLL factors might have pro-angiogenic activity: fibroblast growth factor-2, certain chemokines (such as CXCL-12 and CXCL-2), tumor necrosis factor-α, insulin-like growth factor-1, neutrophil gelatinase-associated lipocalin, and progranulin. All these molecules contribute to the survival, proliferation, and migration of CLL cells. Here, we review the literature on these factors’ respective expression profiles and roles in CLL. We also summarize the main results of preclinical and clinical trials of novel agents targeting most of these molecules in a CLL setting. Through the eradication of leukemic cells and the inhibition of angiogenesis, these therapeutic approaches might alter the course of CLL.
Hepatotoxicity in Cancer Immunotherapy: Diagnosis, Management, and Future Perspectives
Authors: Savino Alberto , Rossi Alberto , Fagiuoli Stefano , Invernizzi Pietro , Gerussi Alessio , Viganò Mauro
Published: January 2025

Cancer immunotherapy, particularly immune checkpoint inhibitors, has positively impacted oncological treatments. Despite its effectiveness, immunotherapy is associated with immune-related adverse events (irAEs) that can affect any organ, including the liver. Hepatotoxicity primarily manifests as immune-related hepatitis and, less frequently, cholangitis. Several risk factors, such as pre-existing autoimmune and liver diseases, the type of immunotherapy, and combination regimens, play a role in immune-related hepatotoxicity (irH), although reliable predictive markers or models are still lacking. The severity of irH ranges from mild to severe cases, up to, in rare instances, acute liver failure. Management strategies require regular monitoring for early diagnosis and interventions, encompassing strict monitoring for mild cases to the permanent suspension of immunotherapy for severe forms. Corticosteroids are the backbone of treatment in moderate and high-grade damage, alone or in combination with additional immunosuppressive drugs for resistant or refractory cases. Given the relatively low number of events and the lack of dedicated prospective studies, much uncertainty remains about the optimal management of irH, especially in the most severe cases. This review presents the main features of irH, focusing on injury patterns and mechanisms, and provides an overview of the management landscape, from standard care to the latest evidence.
Molecular Basis of Oncogenic PI3K Proteins
Authors: Sheng Zhi , Beck Patrick , Gabby Maegan , Habte-Mariam Semhar , Mitkos Katherine
Published: January 2025

The dysregulation of phosphatidylinositol 3-kinase (PI3K) signaling plays a pivotal role in driving neoplastic transformation by promoting uncontrolled cell survival and proliferation. This oncogenic activity is primarily caused by mutations that are frequently found in PI3K genes and constitutively activate the PI3K signaling pathway. However, tumorigenesis can also arise from nonmutated PI3K proteins adopting unique active conformations, further complicating the understanding of PI3K-driven cancers. Recent structural studies have illuminated the functional divergence among highly homologous PI3K proteins, revealing how subtle structural alterations significantly impact their activity and contribute to tumorigenesis. In this review, we summarize current knowledge of Class I PI3K proteins and aim to unravel the complex mechanism underlying their oncogenic traits. These insights will not only enhance our understanding of PI3K-mediated oncogenesis but also pave the way for the design of novel PI3K-based therapies to combat cancers driven by this signaling pathway.
miR-217-5p NanomiRs Inhibit Glioblastoma Growth and Enhance Effects of Ionizing Radiation via EZH2 Inhibition and Epigenetic Reprogramming
Authors: Korleski Jack , Sudhir Sweta , Rui Yuan , Caputo Christopher A. , Sall Sophie , Johnson Amanda L. , Khela Harmon S. , Madhvacharyula Tanmaya , Rasamsetty Anisha , Li Yunqing , Lal Bachchu , Zhou Weiqiang , Smith-Connor Karen , Tzeng Stephany Y. , Green Jordan J. , Laterra John , Lopez-Bertoni Hernando
Published: January 2025

Background/Objectives: CSCs are critical drivers of the tumor and stem cell phenotypes of glioblastoma (GBM) cells. Chromatin modifications play a fundamental role in driving a GBM CSC phenotype. The goal of this study is to further our understanding of how stem cell-driving events control changes in chromatin architecture that contribute to the tumor-propagating phenotype of GBM. Methods: We utilized computational analyses to identify a subset of clinically relevant genes that were predicted to be repressed in a Polycomb repressive complex 2 (PRC2)-dependent manner in GBM upon induction of stem cell-driving events. These associations were validated in patient-derived GBM neurosphere models using state-of-the-art molecular techniques to express, silence, and measure microRNA (miRNA) and gene expression changes. Advanced Poly(β-amino ester) nanoparticle formulations (PBAEs) were used to deliver miRNAs in vivo to orthotopic human GBM tumor models. Results: We show that glioma stem cell (GSC) formation and tumor propagation involve the crosstalk between multiple epigenetic mechanisms, resulting in the repression of the miRNAs that regulate PRC2 function and histone H3 lysine 27 tri-methylation (H3K27me3). We also identified miR-217-5p as an EZH2 regulator repressed in GSCs and showed that miR-217-5p reconstitution using advanced nanoparticle formulations re-activates the PRC2-repressed genes, inhibits GSC formation, impairs tumor growth, and enhances the effects of ionizing radiation in an orthotopic model of GBM. Conclusions: These findings suggest that inhibiting PRC2 function by targeting EZH2 with miR-217-5p advanced nanoparticle formulations could have a therapeutic benefit in GBM.
The Effect of Postoperative Sepsis on 1-Year Mortality and Cancer Recurrence Following Transhiatal Esophagectomy for Esophageal–Gastric Junction Adenocarcinomas: A Retrospective Observational Study
Authors: Faucher Marion , Dahan Samuel , Morel Bastien , de Guibert Jean Manuel , Chow-Chine Laurent , Gonzalez Frédéric , Bisbal Magali , Servan Luca , Sannini Antoine , Tezier Marie , Tourret Maxime , Cambon Sylvie , Pouliquen Camille , Mallet Damien , Nguyen Duong Lam , Ettori Florence , Mokart Djamel
Published: January 2025

Introduction: Transhiatal esophagectomy (THE) is used for specific gastroesophageal junction adenocarcinomas. THE is a high-risk surgical procedure. We aimed to assess the impact of postoperative sepsis (sepsis or septic shock) on the 1-year mortality after THE and to determine the risk factors associated with these outcomes. Secondly, we aimed to assess the impact of postoperative sepsis and other risk factors on 1-year cancer recurrence. Method: A retrospective, observational study was undertaken at the Paoli-Calmettes Institute, Marseille, from January 2012 to March 2022. Results: Of 118 patients, 24.6% (n = 29) presented with postoperative sepsis. Their 1-year mortality was 11% (n = 13), and their 1-year cancer recurrence was 23.7% (n = 28). In the multivariate analysis, independent factors for 1-year mortality were the following: postoperative sepsis (OR: 7.22 (1.11–47); p = 0.038), number of lymph nodes removed (OR: 0. 78 (0.64–0.95); p = 0.011), recurrence at one year (OR: 9.22 (1.66–51.1); p = 0.011), mediastinitis (OR: 17.7 (1.43–220); p = 0.025) and intraoperative driving pressure (OR: 1.77 (1.17–2.68); p = 0.015). For postoperative sepsis, independent factors were low-dose vasopressors (OR: 0.26; 95% CI: 0.07–0.95; p = 0.049), a cervical abscess (OR: 5.33; 95% CI: 1.5–18.9; p = 0.01), bacterial pneumonia (OR: 11.1; 95% CI: 2.99–41.0; p < 0.001) and a high SOFA score on day 1 (OR: 2.65; 95% CI: 1.36–5.19; p = 0.04). For 1-year cancer recurrence, independent factors were the number of lymph nodes removed (sHR: 0.87; 95% CI: 0.79–0.96; p = 0.005), pTNM stages of III or IV (sHR: 8.29; 95% CI: 2.71–25.32; p < 0.001) and postoperative sepsis (sHR: 6.54; 95% CI: 1.70–25.13; p = 0.005). Conclusions: Our study indicates that after THE, postoperative sepsis influences survival and cancer recurrence. We identified the associated risk factors, suggesting an early diagnosis might decrease mortality and recurrence.
Superior Anti-Tumor Response After Microbeam and Minibeam Radiation Therapy in a Lung Cancer Mouse Model
Authors: Subramanian Narayani , Čolić Aleksandra , Santiago Franco Marina , Stolz Jessica , Ahmed Mabroor , Bicher Sandra , Winter Johanna , Lindner Rainer , Raulefs Susanne , Combs Stephanie E. , Bartzsch Stefan , Schmid Thomas E.
Published: January 2025

Objectives: The present study aimed to compare the tumor growth delay between conventional radiotherapy (CRT) and the spatially fractionated modalities of microbeam radiation therapy (MRT) and minibeam radiation therapy (MBRT). In addition, we also determined the influence of beam width and the peak-to-valley dose ratio (PVDR) on tumor regrowth. Methods: A549, a human non-small-cell lung cancer cell line, was implanted subcutaneously into the hind leg of female CD1-Foxn1nu mice. The animals were irradiated with sham, CRT, MRT, or MBRT. The spatially fractionated fields were created using two specially designed multislit collimators with a beam width of 50 μm and a center-to-center distance (CTC) of 400 μm for MRT and a beam width of 500 μm and 2000 μm CTC for MBRT. Additionally, the concept of the equivalent uniform dose (EUD) was chosen in our study. A dose of 20 Gy was applied to all groups with a PVDR of 20 for MBRT and MRT. Tumor growth was recorded until the tumors reached at least a volume that was at least three-fold of their initial value, and the growth delay was calculated. Results: We saw a significant reduction in tumor regrowth following all radiation modalities. A growth delay of 11.1 ± 8 days was observed for CRT compared to the sham, whereas MBRT showed a delay of 20.2 ± 7.3 days. The most pronounced delay was observed in mice irradiated with MRT PVDR 20, with 34.9 ± 26.3 days of delay. Conclusions: The current study highlights the fact that MRT and MBRT modalities show a significant tumor growth delay in comparison to CRT at equivalent uniform doses.
Bi-Hormonal Endocrine Cell Presence Within the Islets of Langerhans of the Human Pancreas Throughout Life
Authors: Hahm Jiwon , Kumar Dawn , Andrade Juan Andres Fernandez , Arany Edith , Hill David J.
Published: January 2025

Bi-hormonal islet endocrine cells have been proposed to represent an intermediate state of cellular transdifferentiation, enabling an increase in beta-cell mass in response to severe metabolic stress. Beta-cell plasticity and regenerative capacity are thought to decrease with age. We investigated the ontogeny of bi-hormonal islet endocrine cell populations throughout the human lifespan. Immunofluorescence microscopy was performed for insulin, glucagon, and somatostatin presence on paraffin-embedded sections of pancreata from 20 donors without diabetes aged between 11 days and 79 years of age. The mean proportional presence of glucagon-, insulin-, and somatostatin-immunoreactive cells within islets was 27.5%, 62.1%, and 12.1%, respectively. There was no change in the relative presence of alpha- or beta-cells with advancing age, but delta-cell presence showed a decline with age (R2 = 0.59, p < 0.001). The most abundant bi-hormonal cell phenotype observed co-stained for glucagon and insulin, representing 3.1 ± 0.3% of all islet cells. Glucagon/somatostatin and insulin/somatostatin bi-hormonal cells were also observed representing 2–3% abundance relative to islet cell number. Glucagon/insulin bi-hormonal cells increased with age (R2 = 0.30, p < 0.05) whilst insulin/somatostatin (R2 = 0.50, p < 0.01) and glucagon/somatostatin (R2 = 0.35, p < 0.05) cells decreased with age of donor. Findings show that bi-hormonal cells are present within human pancreatic islets throughout life, perhaps reflecting an ongoing potential for endocrine cell plasticity.
Dysregulation of the NLRP3 Inflammasome and Promotion of Disease by IL-1β in a Murine Model of Sandhoff Disease
Authors: Platt Nick , Shepherd Dawn , Smith David A. , Smith Claire , Wallom Kerri-Lee , Luqmani Raashid , Churchill Grant C. , Galione Antony , Platt Frances M.
Published: January 2025

Sandhoff disease (SD) is a progressive neurodegenerative lysosomal storage disorder characterized by GM2 ganglioside accumulation as a result of mutations in the HEXB gene, which encodes the β-subunit of the enzyme β-hexosaminidase. Lysosomal storage of GM2 triggers inflammation in the CNS and periphery. The NLRP3 inflammasome is an important coordinator of pro-inflammatory responses, and we have investigated its regulation in murine SD. The NLRP3 inflammasome requires two signals, lipopolysaccharide (LPS) and ATP, to prime and activate the complex, respectively, leading to IL-1β secretion. Peritoneal, but not bone-marrow-derived, macrophages from symptomatic SD mice, but not those from pre-symptomatic animals, secrete the cytokine following priming with LPS with no requirement for activation with ATP, suggesting that such NLRP3 deregulation is related to the extent of glycosphingolipid storage. Dysregulated production of IL-1β was dependent upon caspase activity but not cathepsin B. We investigated the role of IL-1β in SD pathology using two approaches: the creation of hexb−/−Il1r1−/− double knockout mice or by treating hexb−/− animals with anakinra, a recombinant form of the IL-1 receptor antagonist, IL-1Ra. Both resulted in modest but significant extensions in lifespan and improvement of neurological function. These data demonstrate that IL-1β actively participates in the disease process and provides proof-of-principle that blockade of the pro-inflammatory cytokine IL-1β may provide benefits to patients.
A Reverse Design Method for Convective PCR Chips Featuring Precise Control of Steady-State Flow Fields
Authors: Li Chenfei , Xie Yaping , Yong Haochen , Zhao Xin , Ke Xingxing , Wu Zhigang
Published: January 2025

Convective Polymerase Chain Reaction (cPCR), owing to its enhanced thermal cycling efficiency, holds promise for application in the next generation of mainstream commercial PCR instruments. Despite its potential, existing capillary-based and annular reaction chamber designs encounter limitations in precisely controlling the internal flow field, which poses a significant barrier to the progression of cPCR. To overcome these obstacles, this work innovatively proposes a cPCR chip utilizing a “racetrack-shaped” reaction chamber, along with a reverse design approach tailored to meet diverse reaction requirements. Through modeling and simulation, we accurately obtained the relationship between the design parameters and the average flow velocity of the cPCR chip with a “racetrack-shaped” reaction chamber. By capturing the motion of fluorescent particles using a high-speed camera, we acquired the velocity distribution of the actual flow field. Further, we utilized these relationships to conduct a reverse design. Ultimately, a reaction chamber was designed based on the actual amplification needs of 2019-nCoV and hepatitis B virus, and successful amplification was achieved using a self-developed temperature control platform.
Indicators of Glucose Metabolism in Children and Adolescents Characterized as Having “Metabolically Healthy” and “Metabolically Unhealthy” Obesity
Authors: Baltogianni Maria , Dermitzaki Niki , Giapros Vasileios , Balomenou Foteini , Kosmeri Chrysoula , Ladomenou Fani , Kantza Evanthia , Serbis Anastasios
Published: January 2025

Background/Objectives: Some individuals with obesity may exhibit fewer metabolic disturbances and face a lower long-term risk of complications; however, the existence of this so-called “metabolically healthy obesity” (MHO) compared to “metabolically unhealthy obesity” (MUO) remains controversial. We hypothesized that children with MHO might have a more favorable profile than children with MUO. Markers of glucose metabolism and insulin sensitivity were compared between children and adolescents diagnosed with MHO and MUO. Methods: This study recruited prospectively 104 children and adolescents (aged 6–16 years, 47 boys) with obesity. All participants underwent an oral glucose tolerance test (OGTT), and a comparative analysis was performed on HOMA-IR, QUICKI, insulin sensitivity index (ISI), insulinogenic index (IGI), disposition index (DI), and oral disposition index (oDI). Glucose metabolism indices were compared in these subgroups according to pubertal status. Results: Forty-seven children (45.2%) were diagnosed with MHO. The whole-body ISI differed significantly between the MHO and MUO groups (4.02 vs. 2.7, p < 0.01). The IGI was statistically lower in the MHO group compared to MUO (1.26 vs. 1.54, p < 0.01), while neither the DI nor the oDI differed significantly. A higher ISI (4.5 vs. 3.9, p < 0.01) was observed in prepubertal MHO individuals compared to MHO adolescents. Conclusions: Children classified as MHO according to the more recent criteria exhibit a more favorable metabolic profile than those with MUO. However, a completely healthy profile was not demonstrated in the MHO group, as many crucial metabolic profile parameters were comparable to those observed in the MUO group. The findings of this study indicate that all children with obesity, irrespective of whether they are categorized as having MUO or MHO, necessitate close monitoring.
Chronic Microvascular Complications in Sulfonylureas-Treated Diabetic Patients: Correlations with Glycemic Control, Risk Factors and Duration of the Disease
Authors: Confederat Luminita-Georgeta , Stefan Roxana , Condurache Mihaela-Iustina , Dragostin Oana-Maria
Published: January 2025

Background: Diabetes has become one of the most challenging public health problems due to the alarming increase in prevalence and the morbidity and mortality attributed to its acute and chronic complications. Objective: This study aimed to investigate the development of chronic microvascular complications in sulfonylureas-treated diabetic patients and their correlations with glycemic control, risk factors and duration of the disease. Methods: This study included 200 patients that presented to “Providența” Medical Center, Iași. The information was obtained in a retrospective manner based on the observation sheets of the patients. A database was created, analyzed and statistically processed using the Microsoft Excel software (Version 15) and the chi-square test of independence. Results: The prevalence of diabetic polyneuropathy was 33.5%, while diabetic retinopathy was found in 27% of cases. For diabetic polyneuropathy, the results of the statistical analysis demonstrated a statistically significant dependence of the risk factors hyperlipidemia (significance level = 0.01) and overweight/obesity (significance level = 0.05). For diabetic retinopathy, the results demonstrated a statistically significant dependence of the risk factors hypertension (significance level = 0.05) and hyperlipidemia (significance level = 0.01). Conclusions: The present study reveals a strong correlation between the presence of risk factors and the development of microvascular complications of diabetes.
A Comparative Study on Corrosion and Tribocorrosion Behaviors of NiCoCrAlY High-Entropy Alloy Coatings and M50 Steel
Authors: Zeng Qunfeng , Wang Jiahe , Liu Wei , Lin Naiming
Published: January 2025

High-entropy alloy (HEA) coatings have attracted wide scientific attention in academic research and industrial innovation. In the present paper, the NiCoCrAlY HEA coatings are successfully synthesized on the surface of M50 steel to improve the corrosion resistance and tribocorrosion resistance of M50 steel in salt-contaminated lubricating oil. The corrosion and tribocorrosion behaviors of M50 steel and NiCoCrAlY coatings are studied systemically under the same conditions. The experimental results show that NiCoCrAlY coatings have good, densified microstructures and improve effectively the corrosion resistance and tribocorrosion resistance of M50 steel because the protective passivation films and oxide films are formed on the surface of NiCoCrAlY coatings. NiCoCrAlY coatings have high corrosion potential, a low corrosion current density, and a corrosion rate that is comparable with M50 steel. The corrosion potential of M50 steel decreases and the corrosion current density increases with the increase in load due to wear-induced corrosion. The corrosion and tribocorrosion mechanisms of M50 steel and coatings are discussed in light of the experimental results. The wear mechanism of M50 steel is abrasive wear. It is accompanied by corrosion wear for M50 steel and oxidative wear for NiCoCrAlY coatings.
Effect of the Aging Process in the Failure Pressure Estimation in an API 5L Gr. B Cracked Pipeline Using Finite Element Modeling
Authors: Terán Gerardo , Capula-Colindres Selene , C. Velázquez Julio , González-Arévalo Noé E. , Santillán Esther Torres , Angeles-Herrera Daniel , Cervantes-Tobón Arturo
Published: January 2025

This work shows the effect of artificial aging on the mechanical properties of an API 5L Gr. B steel that undergoes different artificial aging times (0, 500, 1250, and 1500 h). Among the mechanical properties studied are the stress–strain curve, yield stress, and ultimate tensile stress. In addition, the parameters from the Ludwik–Hollomon equation, which are the parameter (K) and the work-hardening coefficient (n), are obtained for the true stress–strain curve. Once the true stress–strain curve is plotted, a 3D model of a transverse crack in a pipeline is proposed. The crack defects are straight, and the finite element method (FEM) is used to determine its behavior at different sizes in order to estimate the failure pressure. It can be said that the mechanical properties (stress–strain curve) increase for an aging time of 500 h compared to the air condition because of the over-aging process, something that is well recognized in the literature. In general, past the over-aging condition, with an increase in the aging time, the mechanical properties tend to decrease. This behavior is similar for the failure pressure. The FEM is sensitive to the decrease in mechanical properties along with the true stress–strain curve.
Enhanced Magneto-Optical Kerr Effects in Micron Array Thin Films with Organic–Inorganic Interfaces
Authors: Luo Hao , Wang Ziqi , He Yufei , Song Yujun
Published: January 2025

The modulation of the magneto-optical signal is a crucial research focus in the field of magneto-optics. Micron arrays of varying shapes were fabricated using the UV exposure technique, and their effects were tested on the magneto-optical Kerr response of CoFeB thin films. The influence of the organic–inorganic interface on the magneto-optical Kerr effect (MOKE) was investigated by utilizing organic materials to fabricate arrays. The results indicate that the micron-scale metal array exhibits a limited enhancement of the magneto-optical Kerr signal, but it can change the ferromagnetic strength and the magnetization speed of the magnetic layer. This is because the electron orbit coupling at the organic–inorganic interface enhances the performance of the magnetic film. When a photoresist is used as the array material, the longitudinal magneto-optical Kerr signal of the CoFeB film increases by at least two-fold. The enhancement effect of different shapes of arrays is different because of the different contact area with the CoFeB film.
Predicting High-Grade Acute Urinary Toxicity and Lower Gastrointestinal Toxicity After Postoperative Volumetric Modulated Arc Therapy for Cervical and Endometrial Cancer Using a Normal Tissue Complication Probability Model
Authors: Yang Tianyu , Ji Zhe , Lei Runhong , Qu Ang , Jiang Weijuan , Deng Xiuwen , Jiang Ping
Published: January 2025

(1) Background: Volumetric modulated arc therapy (VMAT) can deliver more accurate dose distribution and reduce radiotherapy-induced toxicities for postoperative cervical and endometrial cancer. This study aims to retrospectively analyze the relationship between dosimetric parameters of organs at risk (OARs) and acute toxicities and provide suggestions for the dose constraints. (2) Methods: A total of 164 postoperative cervical and endometrial cancer patients were retrospectively analyzed, and the endpoints were grade ≥ 2 acute urinary toxicity (AUT) and acute lower gastrointestinal toxicity (ALGIT). The normal tissue complication probability (NTCP) model was established using the logistic regression model. Restricted cubic spline (RCS) curves were used to explore the association between dosimetric parameters and toxicities. The receiver operating characteristic (ROC) curve, calibration curve, Akaike’s corrected information criterion (AICc), decision curve analysis (DCA), and clinical impact curve (CIC) were analyzed to evaluate the performance of NTCP models. (3) Results: Bladder V40Gy was identified to develop the NTCP model of AUT, and the mean AUC was 0.69 (CI: 0.58–0.80). Three candidate predictors, namely the small intestine V30Gy, colon D45%, and rectum D55%, were identified to develop the NTCP model of ALGIT, and the mean AUC was 0.71 (CI: 0.61–0.80). Both models were considered to have relatively good discriminative accuracy and could provide a high net benefit in clinical applications. (4) Conclusions: We developed NTCP models to predict the probability for grade ≥ 2 AUT and ALGIT. We recommend that bladder V40Gy, the small intestine V30Gy, colon D45%, and rectum D55% be controlled below 42%, 20.4%, 16.9 Gy, and 32.0 Gy, respectively.
Feasibility and Preliminary Effects of Acupuncture for Cognitive Dysfunction in Diverse Cancer Survivors: A Pilot, Randomized, Placebo-Controlled Trial
Authors: Li Xiaotong , Lampson Kaitlin , Ahles Tim A. , Root James C. , Li Q. Susan , Li Yuelin , Ahsan Anam , Mao Jun J. , Liou Kevin T.
Published: January 2025

(1) Background: This pilot study evaluates the feasibility and preliminary effects of acupuncture for cancer-related cognitive dysfunction (CRCD) in cancer survivors. (2) Methods: A randomized trial comparing real acupuncture (RA) to sham acupuncture (SA) and waitlist control (WLC) among cancer survivors reporting cognitive difficulties. Interventions were delivered weekly over 10 weeks. Feasibility was evaluated by recruitment, treatment adherence, and assessment completion. Subjective CRCD was assessed by the Functional Assessment of Cancer Therapy-Cognitive Function—Perceived Cognitive Impairment subscale (FACT-Cog PCI) and objective CRCD was assessed by the Hopkins Verbal Learning Test—Revised (HVLT-R). (3) Results: 32 participants (57.1% of eligible patients) were enrolled. All participants in acupuncture groups completed ≥8 of 10 treatments. Assessment completion rate was 100% for all participants. From baseline to week 10, the RA group (n = 19) reported a clinically meaningful 17.3-point increase in FACT-Cog PCI (95% confidence interval [CI] 12.5 to 22.1), compared to 9.7 points (95% CI 2.8 to 16.7) in the SA group (n = 9), and 6.8 points (95% CI −3.7 to 17.2) in the WLC group (n = 4). In the subgroup analysis among patients with a below-average baseline HVLT-R (T-score < 50), the RA group (n = 8) increased FACT-Cog PCI scores by 20.4 (95% CI 13.6 to 27.3), compared to 11.1 points (95% CI 0.6 to 21.5) in the SA group (n = 5). The improvements from RA persisted through week 16 in both the total sample and the sub-group. Eleven mild adverse events were reported, with pain and bleeding at the needling sites being the most common. (4) Conclusions: The findings support the feasibility and safety of conducting a randomized, placebo-controlled trial to evaluate acupuncture for cognitive dysfunction in cancer survivors.
The Prognostic Value of Total Blood Count Parameter Ratios in Acute Pulmonary Embolism
Authors: Yurtseven Aynur , Ensarioğlu Kerem
Published: January 2025

Background/Objectives: Acute pulmonary embolism (PE) is a leading cause of cardiovascular mortality, characterized by nonspecific symptoms and variable clinical presentations. Accurate risk stratification is essential for effective management. While conventional tools like the simplified pulmonary embolism severity index (sPESI) and imaging modalities are widely used, they are often costly and have limitations in predictive accuracy. Inflammatory and coagulative markers, such as the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and mean-platelet-volume-to-lymphocyte ratio (MPVLR), have shown promise in thrombotic conditions. This study explores their prognostic value in PE, focusing on their associations with risk stratification and clinical outcomes. Methods: This retrospective study included 231 adult patients diagnosed with PE at a tertiary care center. Exclusion criteria included recent infection, autoimmune diseases, or immunosuppressive therapy. Laboratory data, clinical parameters, and outcomes (e.g., hospitalization duration, complications, and mortality) were analyzed. Ratios were calculated from routine blood counts, and statistical comparisons were conducted between low- and high-risk groups based on sPESI. Results: High-risk patients (n = 203) exhibited significantly higher troponin, blood urea nitrogen, aspartate aminotransferase, lactate, the NLR (median 4.9 vs. 2.7, p = 0.005), and the MPVLR (median 7.1 vs. 3.9, p = 0.001) compared to low-risk patients. The PLR showed no significant difference between risk groups (p = 0.233). An elevated NLR, PLR, and MPVLR correlated with ICU admission, intubation, and mortality (p < 0.001, p < 0.007, and p < 0.001, respectively). The NLR was the most consistently associated with hospitalization duration and mortality, while the MPVLR and PLR were less predictive of overall hospitalization. Conclusions: The NLR, MPVLR, and PLR are cost-effective, easily calculable markers with the potential for improving risk stratification in PE patients. Among these, the NLR showed the strongest prognostic value, correlating with multiple clinical outcomes. Multicenter studies are needed to validate these findings further and establish clinical utility.