bluuebunny/crossref_metadata_2025 huggingface dataset:
Measurements of Thermal Conductivity of LWC Cement Composites Using Simplified Laboratory Scale Method
Authors: Kurpińska Marzena , Karwacki Jarosław , Maurin Artur , Kin Marek
Published: March 2021

The implementation of low-energy construction includes aspects related to technological and material research regarding thermal insulation. New solutions are sought, firstly, to reduce heat losses and, secondly, to improve the environment conditions in isolated rooms. The effective heat resistance of insulating materials is inversely proportional to temperature and humidity. Cement composites filled with lightweight artificial aggregates may be a suitable material. Selecting a proper method for measuring the thermal conductivity of concrete is important to achieve accurate values for calculating the energy consumption of buildings. The steady state and transient methods are considered the two main thermal conductivity measurement approaches. Steady state is a constant heat transfer, whereby the temperature or heat flow is time independent. In the transient method, temperature changes over time. Most researchers have measured the conductivity of cement-based materials based on transient methods. The availability and cost of equipment, time for experimental measurements and measurement ability for moist specimens may be some of the reasons for using this method. However, considering the accuracy of the measurements, the steady state methods are more reliable, especially for testing dry materials. Four types of composites were investigated that differed in filler: natural aggregate, sintered fly ash filler, sintered clay and granular foam glass aggregate. The method of preparing the samples for testing is especially important for the obtained results. The samples, with a specific surface roughness, will show a lower coefficient of thermal conductivity by 20–30%; therefore, the selection of the type of contact layer between the plate of the measuring device and the sample is of particular importance.
Influence of the 6061 Aluminium Alloy Thermo-Viscoplastic Behaviour on the Load-Area Relation of a Contact
Authors: Rudnytskyj André , Krenn Stefan , Vorlaufer Georg , Gachot Carsten
Published: March 2021

The contact between solids in metal-forming operations often involves temperature-dependent viscoplasticity of the workpiece. In order to estimate the real contact area in such contexts, both the topography and the deformation behaviour should be taken into account. In this work, a deterministic approach is used to represent asperities in appropriately shaped quadratic surfaces. Such geometries are implemented in indentation finite element simulations, in which the indented material has thermo-viscoplastic properties. By creating a database of simulation data, investigations in terms of contact load and area for the specifically shaped asperities allow for an analysis on the influence of the material properties on the load–area relation of the contact. The temperature and viscoplasticity greatly define how much load is supported by a substrate due to an indenting asperity, but the description of the deformation behaviour at small values of strain and strain rate is also relevant. The pile-up and sink-in regions are very dependent on the thermo-viscoplastic conditions and material model, which consequently affect the real contact area calculation. The interplay between carried load and contact area of a full surface analysis indicates the role that different sized asperities play in the contact under different thermomechanical conditions.
The 3D-Printed Honeycomb Metamaterials Tubes with Tunable Negative Poisson’s Ratio for High-Performance Static and Dynamic Mechanical Properties
Authors: Guo Chunxia , Zhao Dong , Liu Zhanli , Ding Qian , Gao Haoqiang , Yan Qun , Sun Yongtao , Ren Fuguang
Published: March 2021

The synthesized understanding of the mechanical properties of negative Poisson’s ratio (NPR) convex–concave honeycomb tubes (CCHTs) under quasi-static and dynamic compression loads is of great significance for their multifunctional applications in mechanical, aerospace, aircraft, and biomedical fields. In this paper, the quasi-static and dynamic compression tests of three kinds of 3D-printed NPR convex–concave honeycomb tubes are carried out. The sinusoidal honeycomb wall with equal mass is used to replace the cell wall structure of the conventional square honeycomb tube (CSHT). The influence of geometric morphology on the elastic modulus, peak force, energy absorption, and damage mode of the tube was discussed. The experimental results show that the NPR, peak force, failure mode, and energy absorption of CCHTs can be adjusted by changing the geometric topology of the sinusoidal element. Through the reasonable design of NPR, compared with the equal mass CSHTs, CCHTs could have the comprehensive advantages of relatively high stiffness and strength, enhanced energy absorption, and damage resistance. The results of this paper are expected to be meaningful for the optimization design of tubular structures widely used in mechanical, aerospace, vehicle, biomedical engineering, etc.
Underwater Image Enhancement Based on Multi-Scale Fusion and Global Stretching of Dual-Model
Authors: Song Huajun , Wang Rui
Published: March 2021

Aimed at the two problems of color deviation and poor visibility of the underwater image, this paper proposes an underwater image enhancement method based on the multi-scale fusion and global stretching of dual-model (MFGS), which does not rely on the underwater optical imaging model. The proposed method consists of three stages: Compared with other color correction algorithms, white-balancing can effectively eliminate the undesirable color deviation caused by medium attenuation, so it is selected to correct the color deviation in the first stage. Then, aimed at the problem of the poor performance of the saliency weight map in the traditional fusion processing, this paper proposed an updated strategy of saliency weight coefficient combining contrast and spatial cues to achieve high-quality fusion. Finally, by analyzing the characteristics of the results of the above steps, it is found that the brightness and clarity need to be further improved. The global stretching of the full channel in the red, green, blue (RGB) model is applied to enhance the color contrast, and the selective stretching of the L channel in the Commission International Eclairage-Lab (CIE-Lab) model is implemented to achieve a better de-hazing effect. Quantitative and qualitative assessments on the underwater image enhancement benchmark dataset (UIEBD) show that the enhanced images of the proposed approach achieve significant and sufficient improvements in color and visibility.
Assessing Machine Learning versus a Mathematical Model to Estimate the Transverse Shear Stress Distribution in a Rectangular Channel
Authors: Lashkar-Ara Babak , Kalantari Niloofar , Sheikh Khozani Zohreh , Mosavi Amir
Published: March 2021

One of the most important subjects of hydraulic engineering is the reliable estimation of the transverse distribution in the rectangular channel of bed and wall shear stresses. This study makes use of the Tsallis entropy, genetic programming (GP) and adaptive neuro-fuzzy inference system (ANFIS) methods to assess the shear stress distribution (SSD) in the rectangular channel. To evaluate the results of the Tsallis entropy, GP and ANFIS models, laboratory observations were used in which shear stress was measured using an optimized Preston tube. This is then used to measure the SSD in various aspect ratios in the rectangular channel. To investigate the shear stress percentage, 10 data series with a total of 112 different data for were used. The results of the sensitivity analysis show that the most influential parameter for the SSD in smooth rectangular channel is the dimensionless parameter B/H, Where the transverse coordinate is B, and the flow depth is H. With the parameters (b/B), (B/H) for the bed and (z/H), (B/H) for the wall as inputs, the modeling of the GP was better than the other one. Based on the analysis, it can be concluded that the use of GP and ANFIS algorithms is more effective in estimating shear stress in smooth rectangular channels than the Tsallis entropy-based equations.
In Search of a Long-Awaited Consensus on Disciplinary Integration in STEM Education
Authors: Aguilera David , Lupiáñez José Luis , Vílchez-González José Miguel , Perales-Palacios Francisco Javier
Published: March 2021

The emergence of STEM (Science, Technology, Engineering, and Mathematics) in research and the practice of science, technology, engineering, and mathematics education is today an unquestionable fact at international level. Despite the importance attached to STEM education, there is a lack of synthesized approaches to teaching the interdisciplinarity of STEM. This lack of synthesis can hinder a strong theoretical foundation for STEM education and possible new contributions. The purpose of this position paper is to contribute a theoretical framework for STEM education that enables the unification of criteria regarding disciplinary integration and associated teaching methods. The authors discuss disciplinary integration in STEM activities, the implication of STEM literacy, educational stage, and teaching method, and provide suggestions for future research.
Diagnosis and Monitoring of Hepatitis B Virus Infection Using the Cobas® HBV Test for Use on the Cobas® 4800 System
Authors: Ortonne Valérie , Wlassow Mélanie , Bouvier-Alias Magali , Melica Giovana , Poveda Jean-Dominique , Laperche Syria , Pawlotsky Jean-Michel , Chevaliez Stephane
Published: March 2021

(1) Background: Sensitive and accurate nucleic acid amplification technologies are now recommended for hepatitis B virus (HBV) DNA detection and quantification in clinical practice to diagnose and monitor hepatitis B infection. The aim of this study was to assess the analytical and clinical performance of the cobas® HBV Test on the cobas® 4800 System. (2) Methods: Standard panel and clinical specimens were tested in parallel with three different real-time commercial PCR assays including the cobas ® HBV Test, the Cobas® AmpliPrep/Cobas® TaqMan HBV Test v2.0 and Alinity™ m HBV assay. (3) Results: The specificity of the cobas® HBV Test was 97.9%. The limit of detection was estimated to be 2.1 IU/mL. Intra-assay and interassay coefficients of variation varied from 0.14% to 1.92% and 2.16% to 12.02%, respectively. HBV DNA levels in patients infected with different HBV genotypes strongly correlated with those measured by the two other commercial comparators assays. (4) Conclusions: The cobas® HBV Test can be confidently used to detect and accurately quantify HBV DNA in clinical practice as well as in clinical trials with the new anti-HBV drugs currently in development.
Detrital Zircon U-Pb Data for Jurassic–Cretaceous Strata from the South-Eastern Verkhoyansk-Kolyma Orogen—Correlations to Magmatic Arcs of the North-East Asia Active Margin
Authors: Prokopiev Andrei V. , Ershova Victoria B. , Stockli Daniel F.
Published: March 2021

We performed U-Pb dating of detrital zircons collected from Middle–Upper Jurassic strata of the Sugoi synclinorium and Cretaceous rocks of the Omsukchan (Balygychan-Sugoi) basin, in order to identify their provenance and correlate Jurassic–Cretaceous sedimentation of the south-eastern Verkhoyansk-Kolyma orogenic belt with various magmatic belts of the north-east Asia active margins. In the Middle–Late Jurassic, the Uda-Murgal magmatic arc represented the main source area of clastics, suggesting that the Sugoi basin is a back-arc basin. A major shift in the provenance signature occurred during the Aptian, when granitoids of the Main (Kolyma) batholith belt, along with volcanic rocks of the Uyandina-Yasachnaya and Uda-Murgal arcs, became the main sources of clastics deposited in the Omsukchan basin. In a final Mesozoic provenance shift, granitoids of the Main (Kolyma) batholith belt, along with volcanic and plutonic rocks of the Uyandina-Yasachnaya and Okhotsk-Chukotka arcs, became the dominant sources for clastics in the Omsukchan basin in the latest Cretaceous. A broader comparison of detrital zircon age distributions in Jurassic–Cretaceous deposits across the south-eastern Verkhoyansk-Kolyma orogen illustrates that the Sugoi and Omsukchan basins did not form along the distal eastern portion of the Verkhoyansk passive margin, but in the Late Mesozoic back-arc basins.
Preface to “Aziridine Chemistry”
Authors: Ha Hyun-Joon
Published: March 2021

Aziridine is a nitrogen-containing three-membered ring with similar ring strain energy as other three-membered ring compounds, including cyclopropane and oxirane [...]
One-Pot Synthesis of Glucose-Derived Carbon Coated Ni3S2 Nanowires as a Battery-Type Electrode for High Performance Supercapacitors
Authors: Wu Zhongkai , Huang Haifu , Xiong Wenhui , Yang Shiming , Huang Huanhuan , Zou Yaohui , Zhou Weiping , Cheng Zhenzhi , Wang Jun , Luo Guangsheng
Published: March 2021

We report a novel Ni3S2 carbon coated (denoted as NCC) rod-like structure prepared by a facile one-pot hydrothermal method and employ it as a binder free electrode in supercapacitor. We coated carbon with glucose as carbon source on the surface of samples and investigated the suitable glucose concentration. The as-obtained NCC rod-like structure demonstrated great performance with a huge specific capacity of 657 C g−1 at 1 A g−1, preeminent rate capability of 87.7% retention, the current density varying to 10 A g−1, and great cycling stability of 76.7% of its original value through 3500 cycles, which is superior to the properties of bare Ni3S2. The result presents a facile, general, viable strategy to constructing a high-performance material for the supercapacitor applications.
Luminescence from Droplet-Etched GaAs Quantum Dots at and Close to Room Temperature
Authors: Ranasinghe Leonardo , Heyn Christian , Deneke Kristian , Zocher Michael , Korneev Roman , Hansen Wolfgang
Published: March 2021

Epitaxially grown quantum dots (QDs) are established as quantum emitters for quantum information technology, but their operation under ambient conditions remains a challenge. Therefore, we study photoluminescence (PL) emission at and close to room temperature from self-assembled strain-free GaAs quantum dots (QDs) in refilled AlGaAs nanoholes on (001)GaAs substrate. Two major obstacles for room temperature operation are observed. The first is a strong radiative background from the GaAs substrate and the second a significant loss of intensity by more than four orders of magnitude between liquid helium and room temperature. We discuss results obtained on three different sample designs and two excitation wavelengths. The PL measurements are performed at room temperature and at T = 200 K, which is obtained using an inexpensive thermoelectric cooler. An optimized sample with an AlGaAs barrier layer thicker than the penetration depth of the exciting green laser light (532 nm) demonstrates clear QD peaks already at room temperature. Samples with thin AlGaAs layers show room temperature emission from the QDs when a blue laser (405 nm) with a reduced optical penetration depth is used for excitation. A model and a fit to the experimental behavior identify dissociation of excitons in the barrier below T = 100 K and thermal escape of excitons from QDs above T = 160 K as the central processes causing PL-intensity loss.
Silver Nanowire Synthesis and Strategies for Fabricating Transparent Conducting Electrodes
Authors: Kumar Amit , Shaikh Muhammad Omar , Chuang Cheng-Hsin
Published: March 2021

One-dimensional metal nanowires, with novel functionalities like electrical conductivity, optical transparency and high mechanical stiffness, have attracted widespread interest for use in applications such as transparent electrodes in optoelectronic devices and active components in nanoelectronics and nanophotonics. In particular, silver nanowires (AgNWs) have been widely researched owing to the superlative thermal and electrical conductivity of bulk silver. Herein, we present a detailed review of the synthesis of AgNWs and their utilization in fabricating improved transparent conducting electrodes (TCE). We discuss a range of AgNW synthesis protocols, including template assisted and wet chemical techniques, and their ability to control the morphology of the synthesized nanowires. Furthermore, the use of scalable and cost-effective solution deposition methods to fabricate AgNW based TCE, along with the numerous treatments used for enhancing their optoelectronic properties, are also discussed.
Silk Fibroin Coated Magnesium Oxide Nanospheres: A Biocompatible and Biodegradable Tool for Noninvasive Bioimaging Applications
Authors: Li Jitao , Khalid Asma , Verma Rajni , Abraham Amanda , Qazi Farah , Dong Xiuxiu , Liang Gaofeng , Tomljenovic-Hanic Snjezana
Published: March 2021

Fluorescent nanoparticles (NPs) have been increasingly studied as contrast agents for better understanding of biological processes at the cellular and molecular level. However, their use as bioimaging tools is strongly dependent on their optical emission as well as their biocompatibility. This work reports the fabrication and characterization of silk fibroin (SF) coated magnesium oxide (MgO) nanospheres, containing oxygen, Cr3+ and V2+ related optical defects, as a nontoxic and biodegradable hybrid platform for bioimaging applications. The MgO-SF spheres demonstrated enhanced emission efficiency compared to noncoated MgO NPs. Furthermore, SF sphere coating was found to overcome agglomeration limitations of the MgO NPs. The hybrid nanospheres were investigated as an in vitro bioimaging tool by recording their cellular uptake, trajectories, and mobility in human skin keratinocytes cells (HaCaT), human glioma cells (U87MG) and breast cancer cells (MCF7). Enhanced cellular uptake and improved intracellular mobilities of MgO-SF spheres compared to MgO NPs was demonstrated in three different cell lines. Validated infrared and bright emission of MgO-SF NP indicate their prospects for in vivo imaging. The results identify the potential of the hybrid MgO-SF nanospheres for bioimaging. This study may also open new avenues to optimize drug delivery through biodegradable silk and provide noninvasive functional imaging feedback on the therapeutic processes through fluorescent MgO.
Enhancement of Luminance in Powder Electroluminescent Devices by Substrates of Smooth and Transparent Cellulose Nanofiber Films
Authors: Tsuneyasu Shota , Watanabe Rikuya , Takeda Naoki , Uetani Kojiro , Izakura Shogo , Kasuya Keitaro , Takahashi Kosuke , Satoh Toshifumi
Published: March 2021

Powder electroluminescent (EL) devices with an electric field type excitation are surface light sources that are expected to have a wide range of practical applications, owing to their high environmental resistance; however, their low luminance has hindered their use. A clarification of the relationship between the properties of the film substrates and the electroluminescence is important to drastically improve light extraction efficiency. In this study, powder EL devices with different substrates of various levels of surface roughness and different optical transmittances were fabricated to quantitatively evaluate the relationships between the substrate properties and the device characteristics. A decrease in the surface roughness of the substrate caused a clear increase in both the current density and the luminance. The luminance was found to have a direct relationship with the optical transmittance of the substrates. The powder EL device, which was based on a cellulose nanofiber film and was the smoothest and most transparent substrate investigated, showed the highest luminance (641 cd/cm2) when 300 V was applied at 1 kHz.
Assessment of Naturally Sourced Mineral Clays for the 3D Printing of Biopolymer-Based Nanocomposite Inks
Authors: Alexa Rebeca Leu , Iovu Horia , Trica Bogdan , Zaharia Catalin , Serafim Andrada , Alexandrescu Elvira , Radu Ionut-Cristian , Vlasceanu George , Preda Silviu , Ninciuleanu Claudia Mihaela , Ianchis Raluca
Published: March 2021

The present study investigated the possibility of obtaining 3D printed composite constructs using biomaterial-based nanocomposite inks. The biopolymeric matrix consisted of methacrylated gelatin (GelMA). Several types of nanoclay were added as the inorganic component. Our aim was to investigate the influence of clay type on the rheological behavior of ink formulations and to determine the morphological and structural properties of the resulting crosslinked hydrogel-based nanomaterials. Moreover, through the inclusion of nanoclays, our goal was to improve the printability and shape fidelity of nanocomposite scaffolds. The viscosity of all ink formulations was greater in the presence of inorganic nanoparticles as shear thinning occurred with increased shear rate. Hydrogel nanocomposites presented predominantly elastic rather than viscous behavior as the materials were crosslinked which led to improved mechanical properties. The inclusion of nanoclays in the biopolymeric matrix limited hydrogel swelling due the physical barrier effect but also because of the supplementary crosslinks induced by the clay layers. The distribution of inorganic filler within the GelMA-based hydrogels led to higher porosities as a consequence of their interaction with the biopolymeric ink. The present study could be useful for the development of soft nanomaterials foreseen for the additive manufacturing of customized implants for tissue engineering.
Epigenetic Regulation of Alternative Splicing: How LncRNAs Tailor the Message
Authors: Pisignano Giuseppina , Ladomery Michael
Published: March 2021

Alternative splicing is a highly fine-tuned regulated process and one of the main drivers of proteomic diversity across eukaryotes. The vast majority of human multi-exon genes is alternatively spliced in a cell type- and tissue-specific manner, and defects in alternative splicing can dramatically alter RNA and protein functions and lead to disease. The eukaryotic genome is also intensively transcribed into long and short non-coding RNAs which account for up to 90% of the entire transcriptome. Over the years, lncRNAs have received considerable attention as important players in the regulation of cellular processes including alternative splicing. In this review, we focus on recent discoveries that show how lncRNAs contribute significantly to the regulation of alternative splicing and explore how they are able to shape the expression of a diverse set of splice isoforms through several mechanisms. With the increasing number of lncRNAs being discovered and characterized, the contribution of lncRNAs to the regulation of alternative splicing is likely to grow significantly.
Cortical Vein Thrombosis after Infliximab Treatment for Crohn’s Disease
Authors: Razmeh Saeed , Niknam Nafiseh , Sadat Rabbani Negar , Nikoee Shekoofeh , Vafa Pour Fatemeh , Taghavian Laleh
Published: March 2021

Inflammatory bowel disease (IBD) including ulcerative colitis and Crohn’s disease puts patients at high risk of thromboembolism accidents. These patients may take infliximab for active and fistulating Crohn’s disease, which can also increase the risk of thrombosis. Deep vein thrombosis (DVT) and pulmonary thromboembolism (PTE) are more common among these patients, but cerebrovascular, mesenteric, portal and retinal veins can also be affected. In this paper, we report a case of isolated right Labbe vein thrombosis after infliximab therapy for Crohn’s disease. To the best of our knowledge, our patient is the first case report of isolated cortical vein thrombosis following administration of rituximab for Crohn’s disease.
Intraperitoneal Administration of Short-Chain Fatty Acids Improves Lipid Metabolism of Long–Evans Rats in a Sex-Specific Manner
Authors: Shah Shrushti , Fillier Tiffany , Pham Thu Huong , Thomas Raymond , Cheema Sukhinder Kaur
Published: March 2021

Short-chain fatty acids (SCFAs) are microbial metabolites, mainly generated by the action of gut microbiota on dietary fibers. Acetate, propionate, and butyrate are the three main SCFAs produced typically in a 60:20:20 molar ratio in the colon. Acetate, propionate, and butyrate, when given individually as supplements, have shown a protective role in obesity and hyperglycemia; however, the sex-specific effects of a mixture of SCFAs, when given in 60:20:20 ratio, on the regulation of lipid metabolism and lipid profile are not known. Male and female Long–Evans rats were given a mixture of SCFAs (acetate, propionate, and butyrate; molar ratio 60:20:20) each day for seven days intraperitoneally; plasma and hepatic lipids, gene expression, and lipidomics profile were analyzed. SCFAs significantly decreased plasma and hepatic triglycerides and cholesterol in males, whereas the fatty acyl composition of cholesteryl esters, triglycerides, and phospholipids was modulated in females. SCFAs decreased the mRNA expression of hepatic acetyl-CoA carboxylase-1 in both males and females. Our findings demonstrate for the first time that SCFAs (60:20:20) improved plasma and hepatic lipid levels and fatty acyl composition in a manner that may provide cardio-protective and anti-inflammatory effects in both sexes, via independent mechanisms.
Gastrointestinal Contributions to the Postprandial Experience
Authors: Livovsky Dan M. , Azpiroz Fernando
Published: March 2021

Food ingestion induces homeostatic sensations (satiety, fullness) with a hedonic dimension (satisfaction, changes in mood) that characterize the postprandial experience. Both types of sensation are secondary to intraluminal stimuli produced by the food itself, as well as to the activity of the digestive tract. Postprandial sensations also depend on the nutrient composition of the meal and on colonic fermentation of non-absorbed residues. Gastrointestinal function and the sensitivity of the digestive tract, i.e., perception of gut stimuli, are determined by inherent individual factors, e.g., sex, and can be modulated by different conditioning mechanisms. This narrative review examines the factors that determine perception of digestive stimuli and the postprandial experience.
Does the Australian Health Star Rating System Encourage Added Sugar Reformulation? Trends in Sweetener Use in Australia
Authors: Russell Cherie , Dickie Sarah , Baker Phillip , Lawrence Mark
Published: March 2021

Dietary risk factors, including excess added sugar intake, are leading contributors to Australia’s burden of disease. An objective of the Australian Health Star Rating (HSR) system is to encourage the reformulation of packaged foods. Manufacturers may improve a product’s HSR by replacing added sugar with non-nutritive sweeteners (NNS). Concerns have been raised regarding the potential substitution effects of ultra-processed foods containing NNS for whole foods, and the long-term impact this may have on population health. The aim of this study was to determine whether the implementation of the HSR system has impacted the use of added sugars and NNS in the food supply. Four product categories were used: products with no added sweetener, products containing added sugar only, products containing NNS only, and products containing a combination of added sugar and NNS. Of 6477 newly released products analyzed displaying a HSR in Australia between 2014–2020, 63% contained added sugars. The proportion of new products sweetened with added sugars increased over time, while NNS use did not, despite a higher average and median HSR for products sweetened with NNS. These findings suggest that at the current time, the HSR system may not discourage the use of added sugars in new products or incentivize the reformulation of added sugar with NNS. As the health risks of NNS are questioned, increased reformulation of products with NNS to reduce the presence of added sugar in the food supply may not address broader health concerns. Instead, supporting the promotion of whole foods and drinks should be prioritized, as well as policy actions that reduce the proliferation and availability of UPFs.
Assessing the Effectiveness of Front of Pack Labels: Findings from an Online Randomised-Controlled Experiment in a Representative British Sample
Authors: Packer Jessica , Russell Simon J. , Ridout Deborah , Hope Steven , Conolly Anne , Jessop Curtis , Robinson Oliver J. , Stoffel Sandro T. , Viner Russell M. , Croker Helen
Published: March 2021

Front of pack food labels (FOPLs) provide accessible nutritional information to guide consumer choice. Using an online experiment with a large representative British sample, we aimed to examine whether FOPLs improve participants’ ability to identify the healthiness of foods and drinks. The primary aim was to compare ability to rank between FOPL groups and a no label control. Adults (≥18 years), recruited from the NatCen panel, were randomised to one of five experimental groups (Multiple Traffic Light, MTL; Nutri-Score, N-S; Warning Label, WL; Positive Choice tick, PC; no label control). Stratification variables were year of recruitment to panel, sex, age, government office region, and household income. Packaging images were created for three versions, varying in healthiness, of six food and drink products (pizza, drinks, cakes, crisps, yoghurts, breakfast cereals). Participants were asked to rank the three product images in order of healthiness. Ranking was completed on a single occasion and comprised a baseline measure (with no FOPL), and a follow-up measure including the FOPL as per each participant’s experimental group. The primary outcome was the ability to accurately rank product healthiness (all products ranked correctly vs. any incorrect). In 2020, 4504 participants had complete data and were included in the analysis. The probability of correct ranking at follow-up, and improving between baseline and follow-up, was significantly greater across all products for the N-S, MTL and WL groups, compared to control. This was seen for only some of the products for the PC group. The largest effects were seen for N-S, followed by MTL. These analyses were adjusted for stratification variables, ethnicity, education, household composition, food shopping responsibility, and current FOPL use. Exploratory analyses showed a tendency for participants with higher compared to lower education to rank products more accurately. Conclusions: All FOPLs were effective at improving participants’ ability to correctly rank products according to healthiness in this large representative British sample, with the largest effects seen for N-S, followed by MTL.
COVID-19 Stress and Food Intake: Protective and Risk Factors for Stress-Related Palatable Food Intake in U.S. Adults
Authors: Sadler Jennifer R. , Thapaliya Gita , Jansen Elena , Aghababian Anahys H. , Smith Kimberly R. , Carnell Susan
Published: March 2021

(1) Background: The coronavirus (COVID-19) pandemic has caused disruptions to what people eat, but the pandemic’s impact on diet varies between individuals. The goal of our study was to test whether pandemic-related stress was associated with food intake, and whether relationships between stress and intake were modified by appetitive and cognitive traits. (2) Methods: We cross-sectionally surveyed 428 adults to examine current intake frequency of various food types (sweets/desserts, savory snacks, fast food, fruits, and vegetables), changes to food intake during the pandemic, emotional overeating (EOE), cognitive flexibility (CF), and COVID-19-related stress. Models tested associations of stress, EOE, and CF with food intake frequency and changes to intake. (3) Results: Models demonstrated that the positive relationship between stress and intake of sweets/desserts was stronger with higher EOE, while the positive relationship between stress and intake of chips/savory snacks was weaker with higher CF. Higher EOE was associated with greater risk of increased intake of palatable foods. (4) Conclusions: Findings suggest that emotional overeating may escalate stress-associated intake of high-sugar foods, and cognitive flexibility may attenuate stress-associated intake of high-fat foods. Differences in appetitive and cognitive traits may explain changes to and variability in food intake during COVID-19, and efforts to decrease emotional overeating and encourage cognitive flexibility could help lessen the effect of COVID-19-related stress on energy dense food intake.
Supplementation with Octacosanol Affects the Level of PCSK9 and Restore Its Physiologic Relation with LDL-C in Patients on Chronic Statin Therapy
Authors: Ciric Milica Zrnic , Ostojic Miodrag , Baralic Ivana , Kotur-Stevuljevic Jelena , Djordjevic Brizita I. , Markovic Stana , Zivkovic Stefan , Stankovic Ivan
Published: March 2021

Dietary supplementation with sugar cane derivates may modulate low-density lipoprotein cholesterol (LDL-C) and proprotein convertase subtilisin/kexin type 9 (PCSK9) levels. The purpose of this study was to determine if dietary supplement (DS), containing Octacosanol (20 mg) and vitamin K2 (45 µg), could restore the disrupted physiologic relation between LDL-C and serum PCSK9. Double-blind, randomized, placebo-controlled, single-center study including 87 patients on chronic atorvastatin therapy was conducted. Eighty-seven patients were randomized to receive DS (n = 42) or placebo (n = 45), and followed for 13 weeks. Serum PCSK9 levels, lipid parameters and their relationship were the main efficacy endpoints. The absolute levels of PCSK9 and LDL-C were not significantly different from baseline to 13 weeks. However, physiologic correlation between % change of PCSK9 and % change of LDL-C levels was normalized only in the group of patients treated with DS (r = 0.409, p = 0.012). This study shows that DS can restore statin disrupted physiologic positive correlation between PCSK9 and LDL-C. Elevated PCSK9 level is an independent risk factor so controlling its rise by statins may be important in prevention of cardiovascular events.
Saffron Extract-Induced Improvement of Depressive-Like Behavior in Mice Is Associated with Modulation of Monoaminergic Neurotransmission
Authors: Monchaux De Oliveira Camille , Pourtau Line , Vancassel Sylvie , Pouchieu Camille , Capuron Lucile , Gaudout David , Castanon Nathalie
Published: March 2021

Depressive disorders represent a major public health concern and display a continuously rising prevalence. Importantly, a large proportion of patients develops aversive side effects and/or does not respond properly to conventional antidepressants. These issues highlight the need to identify further therapeutic strategies, including nutritional approaches using natural plant extracts with known beneficial impacts on health. In that context, growing evidence suggests that saffron could be a particularly promising candidate. This preclinical study aimed therefore to test its antidepressant-like properties in mice and to decipher the underlying mechanisms by focusing on monoaminergic neurotransmission, due to its strong implication in mood disorders. For this purpose, the behavioral and neurobiochemical impact of a saffron extract, Safr’Inside™ (6.5 mg/kg per os) was measured in naïve mice. Saffron extract reduced depressive-like behavior in the forced swim test. This behavioral improvement was associated with neurobiological modifications, particularly changes in serotonergic and dopaminergic neurotransmission, suggesting that Safr’Inside™ may share common targets with conventional pharmacological antidepressants. This study provides useful information on the therapeutic relevance of nutritional interventions with saffron extracts to improve management of mood disorders.
Causal Effects of Homocysteine, Folate, and Cobalamin on Kidney Function: A Mendelian Randomization Study
Authors: Park Sehoon , Lee Soojin , Kim Yaerim , Cho Semin , Kim Kwangsoo , Kim Yong Chul , Han Seung Seok , Lee Hajeong , Lee Jung Pyo , Joo Kwon Wook , Lim Chun Soo , Kim Yon Su , Kim Dong Ki
Published: March 2021

Blood homocysteine level and related vitamin levels are associated with various health outcomes. We aimed to assess causal effects of blood homocysteine, folate, and cobalamin on kidney function in the general population by performing Mendelian randomization (MR) analysis. Genetic instruments for blood homocysteine, folate, and cobalamin levels were introduced from a previous genome-wide association (GWAS) meta-analysis of European individuals. Summary-level MR analysis was performed for the estimated glomerular filtration rate (eGFR) from the CKDGen consortium GWAS that included 567,460 European ancestry individuals. For replication, allele-score-based MR was performed with an independent U.K. Biobank cohort of 337,138 individuals of white British ancestry. In summary-level MR for the CKDGen data, high genetically predicted homocysteine levels were significantly associated with low eGFR (per 1 standard deviation, beta for eGFR change −0.95 (−1.21, −0.69) %), supported by pleiotropy-robust MR sensitivity analysis. Genetically predicted high folate levels were significantly associated with high eGFR change (0.86 (0.30, 1.42) %); however, causal estimates from cobalamin were nonsignificant (−0.11 (−0.33, 0.11) %). In the U.K. Biobank data, the results were consistently identified. Therefore, a high blood homocysteine level causally decreases eGFR. Future trials with appropriate homocysteine-lowering interventions may be helpful for the primary prevention of kidney function impairment.
Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects
Authors: Mio Kento , Otake Nami , Nakashima Satoko , Matsuoka Tsubasa , Aoe Seiichiro
Published: March 2021

The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated. C57BL/6J male mice were fed a high-fat diet supplemented with HGB flour for 90 days. Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA). Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum. Concentrations of short-chain fatty acids (SCFAs) and organic acids were analyzed by GC/MS. Concentrations of sIgA in the cecum and serum were increased in the HGB group compared to the control. Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR) significantly increased in the HGB group. HGB intake increased the bacterial count of microbiota, such as Bifidobacterium and Lactobacillus. Concentrations of propionate and lactate in the cecum were increased in the HGB group, and a positive correlation was found between these organic acids and the IL-10 expression level. Our findings showed that HGB flour enhanced immune function such as IgA secretion and IL-10 expression, even when the immune system was deteriorated by a high-fat diet. Moreover, we found that HGB flour modulated the gut microbiota, which increased the concentration of SCFAs, thereby stimulating the immune system.
Molecular Detection of Selected Pathogens with Zoonotic Potential in Deer Keds (Lipoptena fortisetosa)
Authors: Gałęcki Remigiusz , Jaroszewski Jerzy , Bakuła Tadeusz , Galon Eloiza M. , Xuan Xuenan
Published: March 2021

Deer keds are obligatory hematophagous ectoparasites of birds and mammals. Cervids serve as specific hosts for these insects. However, ked infestations have been observed in non-specific hosts, including humans, companion animals, and livestock. Lipoptena fortisetosa is a weakly studied ectoparasite, but there is evidence to indicate that it continues to spread across Europe. The existing knowledge on deer keds’ impact on wildlife is superficial, and their veterinary importance is enigmatic. Lipoptena fortisetosa is a species with vectorial capacity, but potential pathogen transmission has not been assessed. The objective of this study was to evaluate the prevalence of selected pathogens in L. fortisetosa collected from cervids and host-seeking individuals in the environment. Out of 500 acquired samples, 307 (61.4%) had genetic material from at least one tested pathogen. Our research suggests that L. fortisetosa may be a potential vector of several pathogens, including A. phagocytophilum, Babesia spp., Bartonella spp., Borellia spp., Coxiella-like endosymbionts, Francisiella tularensis, Mycoplasma spp., Rickettsia spp., and Theileria spp.; however, further, more extensive investigations are required to confirm this. The results of the study indicate that keds can be used as biological markers for investigating the prevalence of vector-borne diseases in the population of free-ranging cervids.
Evaluation of “Caterina assay”: An Alternative Tool to the Commercialized Kits Used for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Identification
Authors: Orrù Germano , Scano Alessandra , Fais Sara , Loddo Miriam , Carta Mauro Giovanni , Steri Giorgio Carlo , Santus Simonetta , Cappai Riccardo , Ferrando Maria Laura , Coghe Ferdinando
Published: March 2021

Here we describe the first molecular test developed in the early stage of the pandemic to diagnose the first cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in Sardinian patients in February–March 2020, when diagnostic certified methodology had not yet been adopted by clinical microbiology laboratories. The “Caterina assay” is a SYBR®Green real-time reverse-transcription polymerase chain reaction (rRT-PCR), designed to detect the nucleocapsid phosphoprotein (N) gene that exhibits high discriminative variation RNA sequence among bat and human coronaviruses. The molecular method was applied to detect SARS-CoV-2 in nasal swabs collected from 2110 suspected cases. The study article describes the first molecular test developed in the early stage of the declared pandemic to identify the coronavirus disease 2019 (COVID-19) in Sardinian patients in February–March 2020, when a diagnostic certified methodology had not yet been adopted by clinical microbiology laboratories. The assay presented high specificity and sensitivity (with a detection limit ≥50 viral genomes/μL). No false-positives were detected, as confirmed by the comparison with two certified commercial kits. Although other validated molecular methods are currently in use, the Caterina assay still represents a valid and low-cost detection procedure that could be applied in countries with limited economic resources.
Bartonella henselae Detected in Malignant Melanoma, a Preliminary Study
Authors: Ericson Marna E. , Breitschwerdt Edward B. , Reicherter Paul , Maxwell Cole , Maggi Ricardo G. , Melvin Richard G. , Maluki Azar H. , Bradley Julie M. , Miller Jennifer C. , Simmons Glenn E. , Dencklau Jamie , Joppru Keaton , Peterson Jack , Bae Will , Scanlon Janet , Bemis Lynne T.
Published: March 2021

Bartonella bacilliformis (B. bacilliformis), Bartonella henselae (B. henselae), and Bartonella quintana (B. quintana) are bacteria known to cause verruga peruana or bacillary angiomatosis, vascular endothelial growth factor (VEGF)-dependent cutaneous lesions in humans. Given the bacteria’s association with the dermal niche and clinical suspicion of occult infection by a dermatologist, we determined if patients with melanoma had evidence of Bartonella spp. infection. Within a one-month period, eight patients previously diagnosed with melanoma volunteered to be tested for evidence of Bartonella spp. exposure/infection. Subsequently, confocal immunohistochemistry and PCR for Bartonella spp. were used to study melanoma tissues from two patients. Blood from seven of the eight patients was either seroreactive, PCR positive, or positive by both modalities for Bartonella spp. exposure. Subsequently, Bartonella organisms that co-localized with VEGFC immunoreactivity were visualized using multi-immunostaining confocal microscopy of thick skin sections from two patients. Using a co-culture model, B. henselae was observed to enter melanoma cell cytoplasm and resulted in increased vascular endothelial growth factor C (VEGFC) and interleukin 8 (IL-8) production. Findings from this small number of patients support the need for future investigations to determine the extent to which Bartonella spp. are a component of the melanoma pathobiome.
Pharmacological Interventions for Pulmonary Involvement in Rheumatic Diseases
Authors: Kang Eun Ha , Song Yeong Wook
Published: March 2021

Among the diverse forms of lung involvement, interstitial lung disease (ILD) and pulmonary arterial hypertension (PAH) are two important conditions in patients with rheumatic diseases that are associated with significant morbidity and mortality. The management of ILD and PAH is challenging because the current treatment often provides only limited patient survival benefits. Such challenges derive from their common pathogenic mechanisms, where not only the inflammatory processes of immune cells but also the fibrotic and proliferative processes of nonimmune cells play critical roles in disease progression, making immunosuppressive therapy less effective. Recently, updated treatment strategies adopting targeted agents have been introduced with promising results in clinical trials for ILD ad PAH. This review discusses the epidemiologic features of ILD and PAH among patients with rheumatic diseases (rheumatoid arthritis, myositis, and systemic sclerosis) and the state-of-the-art treatment options, focusing on targeted agents including biologics, antifibrotic agents, and vasodilatory drugs.
Magnetosomes and Magnetosome Mimics: Preparation, Cancer Cell Uptake and Functionalization for Future Cancer Therapies
Authors: Taher Zainab , Legge Christopher , Winder Natalie , Lysyganicz Pawel , Rawlings Andrea , Bryant Helen , Muthana Munitta , Staniland Sarah
Published: March 2021

Magnetic magnetite nanoparticles (MNP) are heralded as model vehicles for nanomedicine, particularly cancer therapeutics. However, there are many methods of synthesizing different sized and coated MNP, which may affect their performance as nanomedicines. Magnetosomes are naturally occurring, lipid-coated MNP that exhibit exceptional hyperthermic heating, but their properties, cancer cell uptake and toxicity have yet to be compared to other MNP. Magnetosomes can be mimicked by coating MNP in either amphiphilic oleic acid or silica. In this study, magnetosomes are directly compared to control MNP, biomimetic oleic acid and silica coated MNP of varying sizes. MNP are characterized and compared with respect to size, magnetism, and surface properties. Small (8 ± 1.6 nm) and larger (32 ± 9.9 nm) MNP are produced by two different methods and coated with either silica or oleic acid, increasing the size and the size dispersity of the MNP. The coated larger MNP are comparable in size (49 ± 12.5 nm and 61 ± 18.2 nm) to magnetosomes (46 ± 11.8 nm) making good magnetosome mimics. All MNP are assessed and compared for cancer cell uptake in MDA-MB-231 cells and importantly, all are readily taken up with minimal toxic effect. Silica coated MNP show the most uptake with greater than 60% cell uptake at the highest concentration, and magnetosomes showing the least with less than 40% at the highest concentration, while size does not have a significant effect on uptake. Finally, surface functionalization is demonstrated for magnetosomes and silica coated MNP using biotinylation and EDC-NHS, respectively, to conjugate fluorescent probes. The modified particles are visualized in MDA-MB-231 cells and demonstrate how both naturally biosynthesized magnetosomes and biomimetic silica coated MNP can be functionalized and readily up taken by cancer cells for realization as nanomedical vehicles.