bluuebunny/crossref_metadata_2025 huggingface dataset:
A Systematic Comparison of the Intrinsic Properties of Wheat and Oat Bran Fractions and Their Effects on Dough and Bread Properties: Elucidation of Chemical Mechanisms, Water Binding, and Steric Hindrance
Authors: Renzetti Stefano , Theunissen Mira , Horrevorts Karlijn
Published: September 2021

This study aimed at elucidating the contribution of chemical interactions, water binding, and steric hindrance on the effect of wheat and oat brans and of their fractions, i.e., soluble and insoluble, on dough and bread properties. For such purpose, an inert filler, i.e., glass beads of comparable particle size and with no water binding capacity and moisture sorption properties, was also studied. The glass beads provided breads most similar to the control, indicating the limited role of steric hindrance. Brans and bran fractions showed distinct compositional and physical properties. The soluble fraction from oat bran, rich in β-glucan, was less hygroscopic than the wheat counterpart and could bind more water, resulting in larger detrimental effects on bread quality. The β-glucan content showed a prevalent role in affecting gluten development, the thermo-setting behaviour of the dough, and crumb texture, i.e., cohesiveness and resilience. Overall, the comparison between the two brans and their fractions indicated that the interplay between water binding, mainly provided by the insoluble fraction, and the plasticizing properties of the soluble bran fraction controlled the effects on bread volume and texture. From a compositional standpoint, β-glucan content was a determining factor that discriminated the effects of wheat and oat brans.
The Blood-Pressure-Lowering Effect of Food-Protein-Derived Peptides: A Meta-Analysis of Recent Clinical Trials
Authors: Liao Wang , Sun Guiju , Xu Dengfeng , Wang Yuanyuan , Lu Yifei , Sun Jihan , Xia Hui , Wang Shaokang
Published: September 2021

Although clinical trials of food-protein-derived peptides in the management of hypertension have been published, the results are controversial, which compelled us to conduct a meta-analysis to evaluate the pooled effect of peptide intervention. In this study, we searched for studies published between 2010 and 2021 and selected 12 eligible studies for a meta-analysis. The pooled effect of peptide intervention for systolic blood pressure (SBP) and diastolic blood pressure (DBP) was −3.28 mmHg (95% CI: −4.54, −2.03, p < 0.001) and −1.82 mmHg (95% CI: −3.46, −0.18, p = 0.03), respectively. Sub-group analyses showed that the reduction in BP in participants with higher basal BP (>140/85 mmHg) was greater (p = 0.007 for SBP and p = 0.01 for DBP), and the effect was stronger in Asian participants as compared with non-Asian participants (p = 0.01 for SBP and p = 0.04 for DBP). In addition, the effect of peptide intervention was more pronounced on SBP in participant groups with a lower ratio of male to female (≤0.5) as well as in participants with a mean age ≥50 years old. In conclusion, food-protein-derived antihypertensive peptides can significantly reduce BP in prehypertensive and hypertensive patients. Findings from this study could provide guidance for the design of clinical trials of antihypertensive peptides.
Surface Cracking and Fractal Characteristics of Bending Fractured Polypropylene Fiber-Reinforced Geopolymer Mortar
Authors: Li Li , Sun Hai-Xin , Zhang Yang , Yu Bo
Published: September 2021

Fiber is effective in restricting cracks and improving the toughness of geopolymer composites, but few studies have focused on the surface crack characteristics of fiber-reinforced geopolymer composites. In this paper, after flexural tests of polypropylene fiber-reinforced geopolymer mortar, the surface cracking image was collected by a digital camera and cracking information was extract by deep learning. Finally, the cracking and fractal characteristics were specifically discussed. The semantic segmentation network can accurately extract surface cracks for calculating various parameters. The results showed that the mean intersection over union (mIoU) and mean pixel accuracy (mPA) of the cracks are 0.8451 and 0.9213, respectively. Generally, the crack length, width, area, and fractal dimension of the specimen are all increased with the increase in the fiber volume fraction. These crack parameters grow rapidly when the fiber content is small, and the growth of the crack parameters gradually slows down as the fiber volume fraction increases to approximately 1.5%. The highest crack parameter values were found in the geopolymer mortar, with a 0.48 water–binder ratio and 12 mm fiber length. The variation of the bottom crack length and the side crack fractal dimension can be used to represent the overall crack variation patterns. Meanwhile, the crack parameters increase with the increased fiber factor in a quadratic function. Based on these crack parameters, the critical fiber factor and dense fiber factor of polypropylene fiber-reinforced geopolymer mortar were 200 and 550, respectively. They are greater than those of fiber-reinforced Portland cementitious composites. The influence of various crack parameters on the flexural strength is in the order of the crack area, width, length, and fractal dimension.
The Distribution of Campylobacter jejuni Virulence Genes in Genomes Worldwide Derived from the NCBI Pathogen Detection Database
Authors: Panzenhagen Pedro , Portes Ana Beatriz , dos Santos Anamaria M. P. , Duque Sheila da Silva , Conte Junior Carlos Adam
Published: September 2021

Campylobacter jejuni (C. jejuni) is responsible for 80% of human campylobacteriosis and is the leading cause of gastroenteritis globally. The relevant public health risks of C. jejuni are caused by particular virulence genes encompassing its virulome. We analyzed 40,371 publicly available genomes of C. jejuni deposited in the NCBI Pathogen Detection Database, combining their epidemiologic metadata with an in silico bioinformatics analysis to increase our current comprehension of their virulome from a global perspective. The collection presented a virulome composed of 126 identified virulence factors that were grouped in three clusters representing the accessory, the softcore, and the essential core genes according to their prevalence within the genomes. The multilocus sequence type distribution in the genomes was also investigated. An unexpected low prevalence of the full-length flagellin flaA and flaB locus of C. jejuni genomes was revealed, and an essential core virulence gene repertoire prevalent in more than 99.99% of genomes was identified. Altogether, this is a pioneer study regarding Campylobacter jejuni that has compiled a significant amount of data about the Multilocus Sequence Type and virulence factors concerning their global prevalence and distribution over this database.
BAZ1B the Protean Protein
Authors: Sharif Shahin Behrouz , Zamani Nina , Chadwick Brian P.
Published: September 2021

The bromodomain adjacent to the zinc finger domain 1B (BAZ1B) or Williams syndrome transcription factor (WSTF) are just two of the names referring the same protein that is encoded by the WBSCR9 gene and is among the 26–28 genes that are lost from one copy of 7q11.23 in Williams syndrome (WS: OMIM 194050). Patients afflicted by this contiguous gene deletion disorder present with a range of symptoms including cardiovascular complications, developmental defects as well as a characteristic cognitive and behavioral profile. Studies in patients with atypical deletions and mouse models support BAZ1B hemizygosity as a contributing factor to some of the phenotypes. Focused analysis on BAZ1B has revealed this to be a versatile nuclear protein with a central role in chromatin remodeling through two distinct complexes as well as being involved in the replication and repair of DNA, transcriptional processes involving RNA Polymerases I, II, and III as well as possessing kinase activity. Here, we provide a comprehensive review to summarize the many aspects of BAZ1B function including its recent link to cancer.
Modelling Physical Accessibility to Public Green Spaces in Switzerland to Support the SDG11
Authors: Chênes Camille , Giuliani Gregory , Ray Nicolas
Published: September 2021

Urban sprawl has a strong impact on the provision and use of green spaces and, consequently, on the benefits that society can derive from these natural ecosystems, especially in terms of public health. In looking at the Sustainable Development Goals and other regional policy frameworks, there is a strong need for quantifying access to green spaces. This study presents and applies a methodology to model the physical accessibility at national and sub-national scales to public green spaces (i.e., urban green spaces and forests) in Switzerland, using AccessMod and ArcGIS travel time functions. We found that approximately 75% and 36% of the Swiss population can access the nearest urban green space within 5 min and 15 min, respectively, using motorized transport. For motorized access to the nearest forest patch, 72% and 52% of the population are within 5 min and 15 min, respectively. When considering only the main urban areas, approximately 55% of the population can walk to the nearest urban green space within 5 min. However, a high heterogeneity in access exists at cantonal and municipal levels, depending on road density, green space density, and population distribution. Despite some possible challenges in correctly delineating public green spaces, our methodology offers a replicable approach offering not only insights into sustainable urban development, but also the facilitation of comparison with other European countries.
The Mechanical Response of a Silty Sand Stabilized with Colloidal Silica
Authors: Vranna Antigoni , Tika Theodora
Published: September 2021

This paper presents a laboratory investigation into the mechanical response of a silty sand, with a fines content of 10%, stabilized with colloidal silica (CS). To this end, a series of unconfined compression tests as well as monotonic and cyclic triaxial tests was performed on a silty sand, comprising a mixture of a clean sand and a silty sand, stabilized with two concentrations of CS. The effect of various parameters on the behaviour of the stabilized silty sand was studied, such as CS concentration, soil density, and the presence of fines. The test results were compared with the corresponding of the untreated silty sand as well as the parent clean sand. It is shown that stabilization, even at the lowest CS = 6% concentration studied, significantly improves the undrained shear strength as well as the liquefaction resistance of the stabilized silty sand. Both the monotonic and cyclic response of the stabilized soil are only slightly affected by density. Furthermore, cyclic straining up to at least 5% of double-amplitude axial strain does not influence the undrained shear strength of the stabilized silty sand.
Stringent Nonpharmaceutical Interventions Are Crucial for Curbing COVID-19 Transmission in the Course of Vaccination: A Case Study of South and Southeast Asian Countries
Authors: Zhao Zebin , Li Xin , Liu Feng , Jin Rui , Ma Chunfeng , Huang Bo , Wu Adan , Nie Xiaowei
Published: September 2021

The ongoing spread of coronavirus disease 2019 (COVID-19) in most South and Southeast Asian countries has led to severe health and economic impacts. Evaluating the performance of nonpharmaceutical interventions in reducing the number of daily new cases is essential for policy designs. Analysis of the growth rate of daily new cases indicates that the value (5.47%) decreased significantly after nonpharmaceutical interventions were adopted (1.85%). Vaccinations failed to significantly reduce the growth rates, which were 0.67% before vaccination and 2.44% and 2.05% after 14 and 28 d of vaccination, respectively. Stringent nonpharmaceutical interventions have been loosened after vaccination drives in most countries. To predict the spread of COVID-19 and clarify the implications to adjust nonpharmaceutical interventions, we build a susceptible–infected–recovered–vaccinated (SIRV) model with a nonpharmaceutical intervention module and Metropolis–Hastings sampling in three scenarios (optimistic, neutral, and pessimistic). The daily new cases are expected to decrease rapidly or increase with a flatter curve with stronger nonpharmaceutical interventions, and the peak date is expected to occur earlier (5–20 d) with minimum infections. These findings demonstrate that adopting stringent nonpharmaceutical interventions is the key to alleviating the spread of COVID-19 before attaining worldwide herd immunity.
The Impact of COVID-19 on the Service of Emergency Department
Authors: Alharthi Shaia , Al-Moteri Modi , Plummer Virginia , Al Thobiaty Abdulellah
Published: September 2021

(1) Introduction: the COVID-19 pandemic significantly impacted the number and acuity of emergency departments (ED) patients, specifically those with non-COVID-19-related health problems. However, the exact impact of the COVID-19 pandemic on ED services is the subject of comprehensive debate. (2) Aim: to gain insight into the consequences of the first wave of the COVID-19 pandemic based on non-COVID-19 presentations and patient acuity using the Canadian Triage and Acuity Scale (CTAS). (3) Method: in Phase 1, the ED records of one of the main regional non-COVID-19 hospitals in Saudi Arabia were retrospectively audited from August 2020 to February 2021—after the first wave of COVID-19—then compared to information collected for the same period in previous year. Phase 2 included calculating the waiting time to identify delays and issues that may impact the triage effectiveness. (4) Results: a change across all CTAS levels was observed post the 1st wave of COVID-19 pandemic. Specifically, there was an increase in the number of patients presenting as higher acuity (CTAS 1 and 2) and a decrease in patients presenting as lower acuity (CTAS 4 and 5). Longer waiting times for patients presenting to ED were also reported. Specifically, 83% of patients presenting as higher acuity experienced a delay. (5) Conclusion: further studies are required to investigate association between the 1st wave of COVID-19 and patient presentations and/or acuity or patient demand and ED capacity.
Comprehensive Energy Renovation of Two Danish Heritage Buildings within IEA SHC Task 59
Authors: Rose Jørgen , Thomsen Kirsten Engelund
Published: September 2021

Historic and heritage buildings present a significant challenge when it comes to reducing energy consumption to mitigate climate change. These buildings need careful renovation, and increasing their energy efficiency is often associated with a high level of complexity, because consideration for heritage values can often reduce and impede possibilities and sometimes even rule out certain improvements completely. Despite these issues, many such renovation projects have already been carried out, and therefore the IEA SHC Task 59 project (Renovating Historic Buildings Towards Zero Energy) in cooperation with Interreg Alpine Space ATLAS has developed a tool for sharing these best-practice examples—the HiBERatlas (Historical Building Energy Retrofit Atlas). The Internet serves as a best-practice database for both individual energy efficiency measures and whole-building renovation projects. This paper presents two of the Danish projects featured in HiBERatlas. The first project, Ryesgade 30, is a Copenhagen apartment building with a preservation-worthy period brick façade. The second project is the Osram Building, a listed Copenhagen office building from 1959 with a protected façade, which today acts as a culture centre. Both renovation projects achieved significant energy savings and consequently CO2-emission reductions, and the indoor climate in both buildings have also improved significantly. Furthermore, a detailed analysis was carried out regarding possible window solutions and ventilation systems in Ryesgade 30, and for the Osram Building regarding daylighting technologies. This paper investigates the two renovation cases through the available measurement and calculation results before and after renovations and demonstrates that it is possible to reduce energy consumption significantly and at the same time improve the indoor climate without compromising the cultural values of buildings.
Multi-Leaf Stone Masonry Walls in Egypt: A Legend
Authors: Amer Osama , Aita Danila , Mohamed Ezzeldin k. , Torky Akram , Shawky Ashraf
Published: September 2021

Multi-leaf stone masonry walls are a typical construction technique in architectural heritage in Egypt. The assessment, modeling, and strengthening of historic masonry walls of multi-leaf systems essentially require suitable knowledge of their construction technology, typology, geometrical characteristics, and the properties of their components. Within the current research project, a comprehensive structural survey of multiple-leaf walls of medieval historic buildings in Cairo was performed. The observation and statistical analyses allowed characterization of the transversal section of the surveyed walls, as well as examination and identification of the construction materials and techniques. The slenderness ratios of this type of wall, its blocks’ dimensions, the utilized connectivity between the inner and outer leaves, and leaves ratio were also investigated. Three construction hypotheses of multiple-leaf stone masonry walls are presented considering weak, thick, and monolithic core infill layers. The study’s objectives were to enlarge the knowledge of typology, morphology, and construction materials used in three-leaf masonry walls and provide a proper characterization as a prerequisite for determining the most suitable materials and techniques for further strengthening interventions.
Study on the Spatiotemporal Evolution and Influencing Factors of Urban Resilience in the Yellow River Basin
Authors: Chen Yu , Su Xuyang , Zhou Qian
Published: September 2021

The outbreak of COVID-19 has prompted consideration of the importance of urban resilience. Based on a multidimensional perspective, the authors of this paper established a comprehensive evaluation indicator system for evaluating urban resilience in the Yellow River basin (YRB), and various methods such as the entropy value method, Theil index, exploratory spatial data analysis (ESDA) model, and geographical detector model were used to measure the spatiotemporal characteristics and influencing factors of urban resilience in the YRB from 2011 to 2018. The results are as follows. (1) From 2011 to 2018, the urban resilience index (URI) of the YRB showed a “V”-shaped dynamic evolution in the time series, and the URI increased by 13.4% overall. The resilience of each subsystem showed the following hierarchical structure: economic resilience > social resilience > ecological resilience > infrastructure resilience. (2) The URI of the three major regions—upstream, midstream, and downstream—increased, and the resilience of each subsystem in the region showed obvious regional characteristics. The comprehensive difference in URI values within the basin was found to be shrinking, and intraregional differences have contributed most to the comprehensive difference. (3) There were obvious zonal differences in the URI from 2011 to 2018. Shandong Peninsula and Hohhot–Baotou–Ordos showed a “High–High” agglomeration, while the southern and southwestern regions showed a “Low–Low” agglomeration. (4) Among the humanist and social factors, economic, fiscal, market, urbanization, openness, and innovation were found to be the factors that exert a high impact on the URI, while the impacts of natural factors were found to be low. The impact of the interaction of each factor is greater than that of a single factor.
On-Site Medical Management of Avalanche Victims—A Narrative Review
Authors: Rauch Simon , Strapazzon Giacomo , Brugger Hermann
Published: September 2021

Avalanche accidents are common in mountain regions and approximately 100 fatalities are counted in Europe each year. The average mortality rate is about 25% and survival chances are mainly determined by the degree and duration of avalanche burial, the patency of the airway, the presence of an air pocket, snow characteristics, and the severity of traumatic injuries. The most common cause of death in completely buried avalanche victims is asphyxia followed by trauma. Hypothermia accounts for a minority of deaths; however, hypothermic cardiac arrest has a favorable prognosis and prolonged resuscitation and extracorporeal rewarming are indicated. In this article, we give an overview on the pathophysiology and on-site management of avalanche victims.
Associations between the School Environment and Physical Activity Pattern during School Time in Swedish Adolescents
Authors: Nyberg Gisela , Ekblom Örjan , Kjellenberg Karin , Wang Rui , Larsson Håkan , Thedin Jakobsson Britta , Helgadóttir Björg
Published: September 2021

Knowledge about associations between school-based initiatives and physical activity patterns is limited. The purpose of this paper was to examine associations between factors in the school environment, physical activity and sedentary time during school time. The cross-sectional study included 1139 adolescents aged 13–14 from 34 schools. Physical activity and sedentary time were measured using hip-worn accelerometers. Factors in the school environment included health policy, a mobile phone ban during breaks, organized physical activities during breaks and activity breaks during lessons reported by teachers. The frequency and duration of breaks and physical education (PE) lessons were collected from school schedules. The results showed significant associations between health policy (β = 3.87, 95% confidence interval (CI): 2.37, 5.23), the mobile phone ban (β = 2.51, 95% CI: 1.29, 3.94) and PE; total duration (β = 0.08, 95% CI: 0.05, 0.11), average duration (β = 0.08, 95% CI: 0.01, 0.13) and frequency (β = 1.73, 95% CI: 0.50, 3.04) and moderate-vigorous physical activity. There were negative associations between health policy (β = −6.41, 95% CI: −10.24, −2.67), the mobile phone ban (β = −3.75, 95% CI: −7.25, −0.77) and PE; total duration (β = −0.15, 95% CI: −0.23, −0.08) and average duration (β = −0.14, 95% CI: −0.27, −0.03) and time spent sedentary. Adolescents attending schools with health policies, mobile phone bans and more time for PE showed higher levels of physical activity and lower sedentary time.
The Immediate and Lasting Effect of Emotion Regulation in Adolescents: An ERP Study
Authors: Yang Meng , Deng Xinmei , An Sieun
Published: September 2021

The immediate effect is an important index of the outcomes of emotion regulation. However, in daily life, whether the effect of emotion regulation lasts and the lasting mechanism have been examined less. The present research focused on the relationships between the immediate and lasting effect of the emotion regulation of adolescents. Electroencephalogram (EEG) was recorded from 51 adolescents (31 boys and 20 girls, Mage = 12.82) during online emotion regulation using the Reactivity and Regulation-Image Task (phase 1) and re-presentation of emotional stimuli after a period of time (phase 2). Event-related potentials (ERPs) related to emotion regulation, such as N2, P3, and the late positive potential (LPP), were examined in the two phases. The results showed that: (1) In both of the two phases, in negative emotion conditions, the amplitudes of P3 and LPP 300–600 of no-regulation conditions were significantly higher than those in reappraisal conditions. However, there was no significant difference under neutral conditions; (2) The amplitudes of P3, N2, and LPP 300–600 during emotion regulation in phase 1 positively predicted the amplitudes of P3, N2, and LPP300–600 in phase 2 in different experimental conditions. Results from the regression analysis implied that the immediate effect of online emotion regulation may predict the lasting effect when adolescents face the same emotions again. In addition, our findings provide neurological evidence that the use of cognitive reappraisal could effectively help adolescents to reduce the recruitment of cognitive resources when they regulate negative emotions and when they face those negative emotions again.
Weight Loss Barriers and Dietary Quality of Intermittent and Continuous Dieters in Women with a History of Gestational Diabetes
Authors: Gray Kristy L. , Clifton Peter M. , Keogh Jennifer B.
Published: September 2021

Weight-loss after gestational diabetes (GDM) lowers the risk of type-2 diabetes (T2DM). Intermittent energy restriction (IER) produces comparable weight-loss to continuous energy restriction (CER), but long-term adherence remains difficult in this population. This exploratory secondary analysis of a 12-month trial comparing IER to CER following GDM examined weight-loss and dietary quality associated with barriers to weight-loss or T2DM risk perception as assessed in a Likert scale questionnaire at baseline. The participants had a median (IQR) BMI of 32.6 (9.4) kg/m2 and 3 (4) years postpartum (n = 121). Forty-five percent (n = 54) of the participants thought they were at a high risk of developing T2DM. Greater affordability of healthy food was related with greater weight-loss at 3 months (p = 0.044, n = 85). At 12 months, there was no significant relationship between weight-loss and the barriers to weight-loss (p > 0.05). CER had superior improvement in dietary quality at 12 months (CER 11 ± 10, IER 6 ± 5.6, n = 42, p = 0.05). Under the Theoretical Domains Framework, the barriers were predominantly related to behavioral regulation (n = 83, 69%; n = 76, 63%) and environmental context and resources (n = 67, 56%). Interventions for diabetes prevention in this population should include behavioral regulation strategies, consider the family home environment, and ensure that the risk of T2DM is conveyed. Women choosing IER may benefit from education to improve their dietary quality.
Assessment of Personal Relaxation in Indoor-Air Environments: Study in Real Full-Scale Laboratory Houses
Authors: Nakayama Yoshitake , Suzuki Norimichi , Nakaoka Hiroko , Tsumura Kayo , Takaguchi Kohki , Takaya Kazunari , Hanazato Masamichi , Todaka Emiko , Mori Chisato
Published: September 2021

The relationship between chemical concentrations in indoor air and the human sense of comfort and relaxation have been reported. We investigated the effect of the sum of volatile organic compounds (ΣVOCs; sum of 79 VOCs) on the level of relaxation in two laboratory houses with almost identical interior and exterior appearances. The electroencephalogram (EEG) was monitored to evaluate the degree of personal relaxation objectively. The experiments were conducted in laboratory houses (LH) A and B with lower and higher levels of ΣVOCs, respectively. A total of 168 healthy volunteers participated, who each performed the task for 20 min, followed by a 10-min break, and EEG was measured during the break. Simultaneously as subjective evaluations, the participants were asked to fill a questionnaire regarding the intensity of odor and preference for the air quality in each LH. The subjective evaluation showed a significant association between ΣVOCs and participants’ relaxation (OR: 2.86, 95%CI: 1.24–6.61), and the objective evaluation indicated that the participants were more relaxed in the LH with lower levels of ΣVOCs than that with higher levels (OR: 3.03, 95%CI: 1.23–7.50). Therefore, the reduction of ΣVOCs and odors in indoor air would have an effect, which is the promotion of relaxation.
Template for Rapid Iterative Consensus of Experts (TRICE)
Authors: Chater Angel M. , Shorter Gillian W. , Swanson Vivien , Kamal Atiya , Epton Tracy , Arden Madelynne A. , Hart Jo , Byrne-Davis Lucie M. T. , Drury John , Whittaker Ellie , Lewis Lesley J. M. , McBride Emily , Chadwick Paul , O’Connor Daryl B. , Armitage Christopher J.
Published: September 2021

Background: Public health emergencies require rapid responses from experts. Differing viewpoints are common in science, however, “mixed messaging” of varied perspectives can undermine credibility of experts; reduce trust in guidance; and act as a barrier to changing public health behaviours. Collation of a unified voice for effective knowledge creation and translation can be challenging. This work aimed to create a method for rapid psychologically-informed expert guidance during the COVID-19 response. Method: TRICE (Template for Rapid Iterative Consensus of Experts) brings structure, peer-review and consensus to the rapid generation of expert advice. It was developed and trialled with 15 core members of the British Psychological Society COVID-19 Behavioural Science and Disease Prevention Taskforce. Results: Using TRICE; we have produced 18 peer-reviewed COVID-19 guidance documents; based on rapid systematic reviews; co-created by experts in behavioural science and public health; taking 4–156 days to produce; with approximately 18 experts and a median of 7 drafts per output. We provide worked-examples and key considerations; including a shared ethos and theoretical/methodological framework; in this case; the Behaviour Change Wheel and COM-B. Conclusion: TRICE extends existing consensus methodologies and has supported public health collaboration; co-creation of guidance and translation of behavioural science to practice through explicit processes in generating expert advice for public health emergencies.
How Can Flowers and Their Colors Promote Individuals’ Physiological and Psychological States during the COVID-19 Lockdown?
Authors: Xie Junfang , Liu Binyi , Elsadek Mohamed
Published: September 2021

The global spread of COVID-19 has disrupted the normality of people’s daily lives, leading the population to social distancing and isolation. The closure of green areas also affected the well-being of the individual during the COVID-19 pandemic. Viewing flowers is expected to have similar positive effects to viewing natural scenery. Therefore, this study investigates how white, red, and yellow flower colors affect individuals’ psychological and physiological well-being. The experiment was conducted in an office-like setting with 50 participants. Participants looked at each flower color for 3 min. Electroencephalograms (EEGs), heart rate variability, and skin conductivity were measured to evaluate physiological responses along with both the semantic differential questionnaire (SD) and the Profile of Mood States (POMS) to assess psychological responses. EEGs showed that the mean values of alpha relative power in the prefrontal lobe were significantly higher when viewing yellow and red flowers vs. white flowers. Furthermore, heart rate variability revealed that viewing yellow and red flowers increased parasympathetic nerve activity significantly. After viewing the yellow and red flowers, the average results for each subscale of the POMS questionnaire improved. The vigor (V) subscale and overall mood status values were significantly improved. The results of the SD method revealed that viewing yellow and red flowers resulted in a significantly higher sense of relaxation, cheerfulness, and comfort than viewing white flowers.
Does Long-Term Training in a Water Immersion Environment Change Interoception?
Authors: Baba Yasuhiro , Sato Daisuke , Otsuru Naofumi , Ikarashi Koyuki , Fujimoto Tomomi , Yamashiro Koya
Published: September 2021

The aim of this study was to investigate individual interoception by comparing the responses of swimmers and baseball players when exposed to specific water environments, depending on training content and environment. Forty-eight healthy male university students were evaluated for their interoceptive response (accuracy, sensibility, and awareness) and heart rate following 25 min of water immersion (WI) at 35 °C. We assessed three conditions: pre-WI, during WI, and post-WI. The results indicated that interoceptive accuracy (IAcc) did not differ between groups because both swimming and baseball do not require emotional expression, as opposed to an activity such as dance. The heart rate was significantly decreased at post-WI compared to that at pre-WI. The IAcc of post-WI presented as higher than that of pre-WI. In addition, there was a significant negative correlation between the ratio of IAcc and that of HR among subjects. Moreover, the attention regulation subscale of the MAIA changed in the WI environment and the ratio of IAcc was negatively correlated with that of the not-distracting subscale of the MAIA. These results suggested that interoception did not differ among the athletes who had long-term training, which enabled them to acquire multi-modal sensorimotor integration, compared to that of non-athlete control participants. We conclude that interoception did not differ among athletes who had long-term training compared to that of non-athlete control participants.
Polypharmacy Exposure, Aging Populations, and COVID-19: Considerations for Healthcare Providers and Public Health Practitioners in Africa
Authors: Nwanaji-Enwerem Jamaji C. , Boyer Edward W. , Olufadeji Ayobami
Published: September 2021

Given the continent’s growing aging population and expanding prevalence of multimorbidity, polypharmacy is an increasingly dire threat to the health of persons living in Africa. The COVID-19 pandemic has only exacerbated these issues. Widespread misinformation, lack of vaccine access, and attempts to avoid being infected have resulted in increases in Africans’ willingness to take multiple prescription and nonprescription medications and supplements. Issues with counterfeit pharmaceuticals and the relatively new recognition of emergency medicine as a specialty across the continent also create unique challenges for addressing this urgent public health need. Experts have called for more robust pharmaceutical regulation and healthcare/public health infrastructure investments across the continent. However, these changes take time, and more near-term strategies are needed to mitigate current health needs. In this commentary, we present a nonexhaustive set of immediately implementable recommendations that can serve as local strategies to address current polypharmacy-related health needs of Africans. Importantly, our recommendations take into consideration that not all healthcare providers are emergency medicine trained and that local trends related to polypharmacy will change over time and require ever-evolving public health initiatives. Still, by bolstering training to safeguard against provider availability biases, practicing evidence-based prescribing and shared decision making, and tracking and sharing local trends related to polypharmacy, African healthcare providers and public health practitioners can better position themselves to meet population needs. Furthermore, although these recommendations are tailored to Africans, they may also prove useful to providers and practitioners in other regions facing similar challenges.
Ginsenoside Rh1 Prevents Migration and Invasion through Mitochondrial ROS-Mediated Inhibition of STAT3/NF-κB Signaling in MDA-MB-231 Cells
Authors: Jin Yujin , Huynh Diem Thi Ngoc , Myung Chang-Seon , Heo Kyung-Sun
Published: September 2021

Breast cancer (BC) a very common cancer in women worldwide. Triple negative breast cancer (TNBC) has been shown to have a poor prognosis with a high level of tumor metastatic spread. Here, the inhibitory effects of ginsenoside-Rh1 (Rh1) on BC metastasis, and its underlying signaling pathway in TNBC were investigated. Rh1-treated MDA-MB-231 cells were analyzed for metastasis using a wound healing assay, transwell migration and invasion assay, western blotting, and qRT-PCR. Rh1 treatment significantly inhibited BC metastasis by inhibiting the both protein and mRNA levels of MMP2, MMP9, and VEGF-A. Further, Rh1-mediated inhibitory effect on BC migration was associated with mitochondrial ROS generation. Rh1 treatment significantly eliminated STAT3 phosphorylation and NF-κB transactivation to downregulate metastatic factors, such as MMP2, MMP9, and VEGF-A. In addition, Mito-TEMPO treatment reversed Rh1 effects on the activation of STAT3, NF-κB, and their transcriptional targets. Rh1 further enhanced the inhibitory effects of STAT3 or NF-κB specific inhibitor, stattic or BAY 11-7082 on MMP2, MMP9, and VEGF-A expression, respectively. In summary, our results revealed the potent anticancer effect of Rh1 on TNBC migration and invasion through mtROS-mediated inhibition of STAT3 and NF-κB signaling.
Adipose-Derived Lipid-Binding Proteins: The Good, the Bad and the Metabolic Diseases
Authors: Frances Laurie , Tavernier Geneviève , Viguerie Nathalie
Published: September 2021

Adipose tissue releases a large range of bioactive factors called adipokines, many of which are involved in inflammation, glucose homeostasis and lipid metabolism. Under pathological conditions such as obesity, most of the adipokines are upregulated and considered as deleterious, due to their pro-inflammatory, pro-atherosclerotic or pro-diabetic properties, while only a few are downregulated and would be designated as beneficial adipokines, thanks to their counteracting properties against the onset of comorbidities. This review focuses on six adipose-derived lipid-binding proteins that have emerged as key factors in the development of obesity and diabetes: Retinol binding protein 4 (RBP4), Fatty acid binding protein 4 (FABP4), Apolipoprotein D (APOD), Lipocalin-2 (LCN2), Lipocalin-14 (LCN14) and Apolipoprotein M (APOM). These proteins share structural homology and capacity to bind small hydrophobic molecules but display opposite effects on glucose and lipid metabolism. RBP4 and FABP4 are positively associated with metabolic syndrome, while APOD and LCN2 are ubiquitously expressed proteins with deleterious or beneficial effects, depending on their anatomical site of expression. LCN14 and APOM have been recently identified as adipokines associated with healthy metabolism. Recent findings on these lipid-binding proteins exhibiting detrimental or protective roles in human and murine metabolism and their involvement in metabolic diseases are also discussed.
Characterization of Human Medullary Thyroid Carcinoma Glycosphingolipids Identifies Potential Cancer Markers
Authors: Säljö Karin , Thornell Anders , Jin Chunsheng , Norlén Olov , Teneberg Susann
Published: September 2021

Medullary thyroid carcinoma (MTC) accounts for only 1–2% of thyroid cancers; however, metastatic MTC is a mortal disease with no cure. In this study, glycosphingolipids were isolated from human MTCs and characterized by mass spectrometry and binding of carbohydrate recognizing ligands. The tissue distribution of selected compounds was investigated by immunohistochemistry. The amount of acid glycosphingolipids in the MTCs was higher than in the normal thyroid glands. The major acid glycosphingolipid was the GD3 ganglioside. Sulfatide and the gangliosides GM3 and GD1a were also present. The majority of the complex non-acid glycosphingolipids had type 2 (Galβ4GlcNAc) core chains, i.e., the neolactotetraosylceramide, the Lex, H type 2 and x2 pentaosylceramides, the Ley and A type 2 hexaosylceramides, and the A type 2 heptaosylceramide. There were also compounds with globo (GalαGalβ4Glc) core, i.e., globotriaosylceramide, globotetraosylceramide, the Forssman pentaosylceramide, and the Globo H hexaosylceramide. Immunohistochemistry demonstrated an extensive expression av Ley in the MTC cells and also a variable intensity and prevalence of Globo H and Lex. One individual with multiple endocrine neoplasia type 2B expressed the Forssman determinant, which is rarely found in humans. This study of human MTC glycosphingolipids identifies glycans that could serve as potential tumor-specific markers.
Are Non-Coding RNAs Useful Biomarkers in Parathyroid Tumorigenesis?
Authors: Aurilia Cinzia , Donati Simone , Palmini Gaia , Miglietta Francesca , Falsetti Irene , Iantomasi Teresa , Brandi Maria Luisa
Published: September 2021

Tumors of the parathyroid glands are common endocrine diseases almost always characterized by parathyroid hormone hypersecretion that determines the clinical manifestations of primary hyperparathyroidism, such as fatigue, kidney problems, weakness, brittle bones, and other symptoms. Most parathyroid neoplasia are benign adenomas, although rare malignant forms have been described. They are heterogeneous in terms of clinical presentation and the associated signs and symptoms overlap with those of disease and aging. Furthermore, most patients with hypercalcemia are discovered during routine blood tests for other reasons. Surgical removal is considered the main therapeutic option to cure these endocrine tumors and, therefore, innovative therapeutic approaches are actively required. Recently, a growing number of studies have suggested that alterations to the epigenetic mechanisms could play a pivotal role in parathyroid tumorigenesis. Most of the attention has been focused on non-coding RNAs (ncRNAs) (i.e., miRNAs, lncRNAs, and circRNAs) whose expression profile has been found to be deregulated in parathyroid tumors. The aim of the present paper is to give an insight into the ncRNAs involved in parathyroid tumorigenesis, which could be used in the future either as innovative diagnostic biomarkers or as therapeutic targets for the treatment of this endocrine neoplasia.
Untargeted Metabolic Profiling of Extracellular Vesicles of SARS-CoV-2-Infected Patients Shows Presence of Potent Anti-Inflammatory Metabolites
Authors: Alzahrani Faisal A. , Shait Mohammed Mohammed Razeeth , Alkarim Saleh , Azhar Esam I. , El-Magd Mohammed A. , Hawsawi Yousef , Abdulaal Wesam H. , Yusuf Abdulaziz , Alhatmi Abdulaziz , Albiheyri Raed , Fakhurji Burhan , Kurdi Bassem , Madani Tariq A. , Alguridi Hassan , Alosaimi Roaa S. , Khan Mohammad Imran
Published: September 2021

Extracellular vesicles (EVs) carry important biomolecules, including metabolites, and contribute to the spread and pathogenesis of some viruses. However, to date, limited data are available on EV metabolite content that might play a crucial role during infection with the SARS-CoV-2 virus. Therefore, this study aimed to perform untargeted metabolomics to identify key metabolites and associated pathways that are present in EVs, isolated from the serum of COVID-19 patients. The results showed the presence of antivirals and antibiotics such as Foscarnet, Indinavir, and lymecycline in EVs from patients treated with these drugs. Moreover, increased levels of anti-inflammatory metabolites such as LysoPS, 7-α,25-Dihydroxycholesterol, and 15-d-PGJ2 were detected in EVs from COVID-19 patients when compared with controls. Further, we found decreased levels of metabolites associated with coagulation, such as thromboxane and elaidic acid, in EVs from COVID-19 patients. These findings suggest that EVs not only carry active drug molecules but also anti-inflammatory metabolites, clearly suggesting that exosomes might play a crucial role in negotiating with heightened inflammation during COVID-19 infection. These preliminary results could also pave the way for the identification of novel metabolites that might act as critical regulators of inflammatory pathways during viral infections.
The Ephb2 Receptor Uses Homotypic, Head-to-Tail Interactions within Its Ectodomain as an Autoinhibitory Control Mechanism
Authors: Xu Yan , Robev Dorothea , Saha Nayanendu , Wang Bingcheng , Dalva Matthew B. , Xu Kai , Himanen Juha P. , Nikolov Dimitar B.
Published: September 2021

The Eph receptor tyrosine kinases and their ephrin ligands direct axon pathfinding and neuronal cell migration, as well as mediate many other cell–cell communication events. Their dysfunctional signaling has been shown to lead to various diseases, including cancer. The Ephs and ephrins both localize to the plasma membrane and, upon cell–cell contact, form extensive signaling assemblies at the contact sites. The Ephs and the ephrins are divided into A and B subclasses based on their sequence conservation and affinities for each other. The molecular details of Eph–ephrin recognition have been previously revealed and it has been documented that ephrin binding induces higher-order Eph assemblies, which are essential for full biological activity, via multiple, distinct Eph–Eph interfaces. One Eph–Eph interface type is characterized by a homotypic, head-to-tail interaction between the ligand-binding and the fibronectin domains of two adjacent Eph molecules. While the previous Eph ectodomain structural studies were focused on A class receptors, we now report the crystal structure of the full ectodomain of EphB2, revealing distinct and unique head-to-tail receptor–receptor interactions. The EphB2 structure and structure-based mutagenesis document that EphB2 uses the head-to-tail interactions as a novel autoinhibitory control mechanism for regulating downstream signaling and that these interactions can be modulated by posttranslational modifications.
Comparative and Systematic Omics Revealed Low Cd Accumulation of Potato StMTP9 in Yeast: Suggesting a New Mechanism for Heavy Metal Detoxification
Authors: Li Dandan , He Guandi , Tian Weijun , Saleem Muhammad , Huang Yun , Meng Lulu , Wu Danxia , He Tengbing
Published: September 2021

The metal tolerance protein (MTP) family is a very old family with evolutionary conservation and less specific amplification. It seems to retain the original functions of the ancestral genes and plays an important role in maintaining metal homeostasis in plant cells. We identified the potato MTP family members for the first time, the specific and conservative StMPTs were discovered by using systematic and comparative omics. To be surprised, members of the StMTP family seem to have mutated before the evolution of dicotyledon and monocotyledon, and even the loss of the entire subfamily (subfamily G6, G7). Interestingly, StMTP9 represents the conserved structure of the entire subfamily involved in toxic metal regulation. However, the gene structure and transmembrane domain of StMTP8 have undergone specific evolution, showing that the transmembrane domain (Motif13) located at the NH2 terminal has been replaced by the signal peptide domain, so it was selected as the control gene of StMTP9. Through real-time fluorescence quantitative analysis of StMTPs under Cd and Zn stress, a co-expression network was constructed, and it was found that StMTP9 responded significantly to Cd stress, while StMTP8 did the opposite. What excites us is that by introducing StMTPs 8/9 into the ∆ycf1 yeast cadmium-sensitive mutant strain, the functional complementation experiment proved that StMTPs 8/9 can restore Cd tolerance. In particular, StMTP9 can greatly reduce the cadmium content in yeast cells, while StMTP8 cannot. These findings provide a reference for further research on the molecular mechanism of potato toxic metal accumulation.