bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of Availability and Use of Teaching and Learning Resources for Retention of Learners with Special Needs in Regular Primary Schools in Dadaab Sub-County, Kenya
Authors: Osman Dure Mohammed , P. A. Oracha , Joel Okutoyi
Published: June 2020

The status of implementation of inclusive education in terms of availability and use of teaching and learning resources for retention of learners with special needs in Garissa County was unknown. Inclusive education refers to a situation where learners with disabilities and special educational needs have full membership in age-appropriate classes in their regular neighborhood schools with appropriate supplementary aids and support services. Records from Educational Assessment and Resource Centres (EARC) Garissa County revealed that there was a decline in the retention rate of learners` admitted. For example, in the year 2014, the retention rate was 37 learners (45.7%), 2015 (114 learners, 38.0%), 2016 (43 learners, 28.9%), 2017 (35 learners, 31.5%) and 2018 (41 learners, 23.7%). The purpose of the study was to assess the status of implementation of inclusive education practices for the retention of learners with special needs in regular primary schools in Dadaab sub-county. The specific objective of the study was to establish the extent to which the availability and use of teaching and learning resources affect the retention of learners with special needs in regular primary schools in Dadaab sub-county. The study adopted a descriptive survey research design. The target population comprised 26 head-teachers, 78 teachers, 300 learners with special needs, and 4 education officers. Saturated sampling technique was used to select 23 head-teachers, 70 teachers, 100 learners with special needs, and 4 education officers. Instruments for data collection were questionnaires, interview schedules and observation schedules. Quantitative data were analyzed using frequency counts, percentages, and mean. The findings of the study indicated that the availability and use of teaching and learning materials were inadequate (mean= 2.18). The study concluded that the low retention of learners with special needs as a result of the availability and use of teaching and learning materials to a small extent.  The study recommended that the country and national government should ensure that there is adequate provision of teaching and learning resources to enhance learning for learners with special needs. The budget for materials should be enhanced. The study also recommends to non-governmental organizations to assist regular primary schools to access teaching and learning materials that can be used by learners with special needs.
PROBLEM DOKTRIN RASISME ETNIS CHINA SEBAGAI WNI (STATUS KEWARGANEGARAAN ETNIS CHINA)
Authors: Sunaryo Sidik , Purnamawati Shinta Ayu
Published: June 2020

Keanekaragaman harus menjadi kekayaan dan bukan hanya sekedar beda yang satu. Tulisan ini mencoba menjelaskan problem doktrin hukum formal Negara (Konstitusi) yang mengatur tentang hubungan antara Ras (Keturunan atau Etnik) yang ada di Indonesia. Hukum tentang pengaturan Ras (Keturunan atau Etnik) di Indonesia, tidak bisa dilihat dari perspektif  baik dan buruk saja, tetapi hukum pengaturan Ras (Keturunan atau Etnik) di Indonesia harus dilihat dalam perspektif benar dan salah. Antara ketentuan satu dengan yang lainnya masih saling bertentangan yang menjadi penyebab problem konstitusional ketidakteraturan hukum yang mengatur jaminan hak dan kewajiban bagi WNI ras dan etnis China secara sejajar dengan WNI asli. Pembedaan WNI asli dan tidak asli menjadi bukti problem ketidakteraturan hukum yang menjustifikasi Rasisme di Indonesia. Hal ini mengakibatkan perbedaan perlakuan dari penyelenggara Negara terhadap WNI tidak asli dari ras dan etnis China dalam bidang sosial, budaya, agama, kependudukan dan keimigrasian.
El hogar y el héroe: Espacios y subjetividades en True Detective (HBO, 2014)
Authors: Rodríguez Serrano Aarón , García Catalán Shaila
Published: June 2020

La primera temporada de True Detective, más allá del revuelo desatado en redes sociales, planteaba interesantes cuestiones de fondo sobre las relaciones que se establecen entre los espacios que habitamos, las quiebras que surgen en la definición de lo doméstico y su relación con las acciones heroicas. Nuestro trabajo pretende ahondar entre esos tres vectores, poniendo especial hincapié en la manera en la que la forma audiovisual televisiva traduce y aborda las hendiduras en la experiencia de la familia y la justicia para el sujeto contemporáneo. No pretendemos basarnos únicamente en las lecturas heredadas de la narratología heroica clásica (Vladimir Propp, Joseph Campbel…), sino preguntarnos por la manera en la que ciertos recursos audiovisuales concretos (el plano secuencia, el uso del contraplano…) generan una significación determinada, ambigua pero estimulante, a propósito de las experiencias y los modos del habitar. A modo concreto de ejemplo, utilizaremos principalmente el célebre plano secuencia incorporado en el cuarto episodio de la primera temporada, un ejemplo especialmente útil para plantearnos no solo por las posibilidades de significación de ciertos recursos audiovisuales, sino también por la manera en la que dicho plano fue entendido como una suerte de “descubrimiento” por parte de una audiencia poco acostumbrada a los estilemas de la modernidad. En cierto sentido, True Detective nos parece un objeto de estudio especialmente relevante para plantear estas cuestiones en tanto contrapone dos modelos canónicos de personajes y de espacios: del héroe familiar/integrado –que señorea, aparentemente, en la seguridad tradicional de lo doméstico- y el héroe outsider/apocalíptico, desplazado aparentemente al lugar externo del relato, un territorio marginal. Sin embargo, es precisamente en ese territorio marginal en el que emerge la posibilidad de levantar una Verdad y celebrar un Acontecimiento que, precisamente por su eficacia simbólica, ponga en crisis no sólo la concepción misma de lo heroico, sino también la propia estructura invertida de la Ley, mostrando así a todas luces su obscenidad y sus íntimas contradicciones..
El éxito de los podcasts de noticias y su impacto en los medios de comunicación digital
Authors:   Universidad de Navarra, Martínez-Costa Pérez María Pilar , Lus Gárate Eva ,   Universidad de Navarra
Published: June 2020

Los podcasts de noticias están acaparando el interés de las empresas periodísticas a medida que crece su consumo, que se espera mayor a medida que se generalice la implantación de los altavoces inteligentes. Estas nuevas modalidades de acceso a los contenidos informativos abren nuevas oportunidades narrativas para contar las noticias a través del audio y pueden ayudar a consolidar el prestigio informativo de las grandes marcas periodísticas en el entorno digital. Este trabajo es una primera aproximación teórica al fenómeno de los denominados daily news podcasts o podcast de noticias, su definición, sus características, sus tipologías y sus peculiaridades narrativas. A partir de la revisión bibliográfica de este formato novedoso y del análisis de contenido de tres casos —The Daily (The New York Times), Today in Focus (The Guardian) y Las noticias de ABC (Vocento)— se concluirá que el éxito de los podcasts de noticias permitirá a los medios de comunicación digital llegar a nuevas audiencias, crear comunidades de marca a partir del audio, explorar nuevas narrativas y abrir nuevas oportunidades de negocio.
Radiografía de las revistas universitarias de divulgación científica en España: análisis de los temas y las fuentes
Authors: Santos Maestre Lorena
Published: June 2020

La investigación, junto con la docencia, es el motor de las universidades. Es importante obtener buenos resultados, pero también difundirlos. Por este motivo, las universidades españolas ponen en marcha revistas divulgativas que trasladan al público la investigación que se lleva a cabo en la propia institución. Este conjunto de publicaciones, que suponen el primer eslabón de comunicación de la ciencia, son una muestra de estudio idónea para analizar las principales características de la divulgación científica española. El principal objetivo del trabajo es establecer la situación actual de este tipo de divulgación científica realizada en las universidades españolas. Desde la universidad es posible difundir ciencia, por la accesibilidad a las fuentes y por la cantidad de materia prima que se elabora en estas instituciones. Mediante métodos cuantitativos y cualitativos, se examina en primer lugar la existencia de publicaciones de divulgación universitaria y, en segundo lugar, el formato, las temáticas, las fuentes y la periodicidad de dichas revistas. Además, se analiza en profundidad el uso de las fuentes y la preponderancia de los temas en las diferentes revistas. Finalmente, se proponen algunas mejoras que ayudarían a mejorar la tarea de divulgación que realizan estas publicaciones.
A Semianalytical Method for Two-Phase Flowback Rate-Transient Analysis in Shale Gas Reservoirs
Authors: Zhang Fengyuan , Emami-Meybodi Hamid
Published: June 2020

We propose a new semianalytical method for analyzing flowback water and gas production data to estimate hydraulic fracture (HF) properties and to quantify HF dynamics. The method includes a semianalytical flowback model, a set of two-phase diagnostic plots, and a workflow to evaluate initial fracture volume and permeability, as well as fracture compressibility and permeability modulus. The flowback model incorporates two-phase water and gas flow in both HF and matrix domains and considers variations of fluid and rock properties with pressure. The HF domain is modeled by boundary-dominated flow, whereas an infinite-acting linear flow is assumed for the matrix domain. The flowback model is developed by assigning the variable average pressure in the fracture as the inner boundary condition for matrix according to Duhamel's principle. The average pressure in the fracture and distance of investigation (DOI) in the matrix are calculated from a modified material-balance equation by updating the matrix DOI as well as phase saturation and relative permeability in both the fracture and matrix domains. A modified DOI equation is used for two-phase flow in the matrix, which considers the pressure-dependent fluid and rock properties in pseudotime. The diagnostic plots shed light on the identification of flow regimes during the coupled two-phase flow in both fracture and matrix. The proposed workflow quantifies the HF dynamics through the loss of both fracture volume and fracture permeability by reconciling flowback and long-term production data. The accuracy of the new method is tested against numerical simulations conducted by a commercial numerical simulator. The validation results confirm that the proposed method accurately predicts initial fracture volume, permeability, and permeability modulus. Further, we use production data from a multifractured horizontal well (MFHW) drilled in Marcellus Shale to test the practicality of the proposed method. The results show a significant reduction in fracture volume and permeability during production attributable to the HF closure.
Relationship between aspirin use of esophageal, gastric and colorectal cancer patient survival: a meta-analysis
Authors: Lin Juli , Lin Jian-xian , Zheng Chao-hui , Li Ping , Xie Jian-wei , Wang Jia-bin , Lu Jun , Chen Qi-yue , Cao Long-Long , Lin Mi , Huang Chang-ming
Published: June 2020

Background: Many studies have found that use of aspirin can lengthen survival in patients with gastrointestinal cancer. The aim of this study was to assess the survival benefit of aspirin use compared with non-aspirin use for patients with esophageal, gastric or colorectal cancer.Methods: We searched online databases, including PubMed, the Cochrane Library, Embase and www.clinicaltrials.gov for studies that were conducted, before April 30th, 2020, to identify relevant studies. Overall survival and cancer-specific survival of esophageal, gastric and colorectal cancers among aspirin users were compared with those among non-aspirin users. Data extraction and quality evaluation were independently conducted by 2 investigators. A meta-analysis was performed to calculate the pooled risk ratios (RRs) for overall survival and cancer-specific survival by using either a fixed-effects model or a random-effects model.Results: A total of 18 studies were included in this meta-analysis, with more than 74,936 patients. There were no significant differences between postdiagnosis aspirin use and overall survival for esophageal and gastric cancers. For colorectal cancer, a benefit that was associated with postdiagnosis aspirin use was observed for overall survival and cancer-specific survival [HR= 0.83, 95%CI(0.75, 0.9.);HR= 0.78, 95%CI(0.66, 0.92), respectively. However, a prediagnosis of aspirin use did not provide a benefit for overall or cancer-specific survival in colorectal cancer. HR values for overall and cancer-specific survival benefits for colorectal cancer associated with both prediagnosis and postdiagnosis aspirin were as follows: HR=0.75，95%CI(0.61, 0.92) and HR=0.78, 95%CI(0.73, 0.85), respectively. In addition, the survival benefit of postdiagnosis aspirin use appeared to be confined to patients with mutated PIK3CA tumors [HR= 0.78, 95%CI(0.50, 0.99)] and was positive for PTGS2 (COX-2) expression [HR= 0.75, 95%CI(0.43, 1.30)].Conclusions: These findings provide further indications that postdiagnosis aspirin use improves overall survival and cancer-specific survival in colorectal cancer, especially for patients who are positive for PTGS2 (COX-2) expression and PIK3CA-mutated tumors. However, aspirin therapy does not improve overall survival in esophageal and gastric cancers, although the meta-analysis was mainly limited to retrospective studies.
Importance of congruence between communicating and executing implementation programmes: A qualitative study of focus-group interviews.​
Authors: Lindholm Lars H. , Laitila Minna , Lassila Antero , Kampman Olli
Published: June 2020

Background
The Ostrobothnia Depression Programme (ODP) in Finland was intended to implement two evidence-based brief psychotherapy interventions, namely motivational interview and behavioural activation, in several regional psychiatric teams. A simultaneous effectiveness study was conducted. Considerable tension was encountered between these two arms, causing resistance to change. We conducted a qualitative case study to better understand this tension and to ascertain how managerial and executive practices may ensure the successful running of a hybrid design programme.

Methods
We conducted focus group interviews to evaluate the phases of preparation and practical execution of the ODP from the perspectives of management and the programme executives. To gather the data, we applied the revised Socratic approach for health technology assessment and the rapid assessment process model. We analysed the data deductively according to Normalization Process Theory.

Results
We identified two main critical issues: 1) Incongruence occurred between what the project group had explicitly communicated about being the spearhead of the ODP and what they then actually enacted. 2) The ODP programme plan ignored the team leaders' crucial role in influencing the implementation climate and mobilizing organizational strategies. The ODP had a simplistic top-down design with minimal and delayed collaboration with its target groups in the preparation phase. These caused tension between the implementation efforts and the effectiveness study as well as resistance to change among the staff.

Conclusion
Early, open collaboration with all prospective stakeholders towards a shared understanding about the programme is the first action the programme administrators should take. Agreement on goals and the means to achieve them would lower tension between the two arms of a hybrid design programme, thereby reducing resistance to change. Congruence between the goals communicated and the actual managerial and executive actions is of paramount importance in getting the programme recipients on board.
Comparative transcriptome analysis between inbred and hybrids reveals molecular insights into yield heterosis of upland cotton
Authors: Shahzad Kashif , Zhang Xuexian , Guo Liping , Qi Tingxiang , Bao Lisheng , Zhang Meng , Zhang Bingbing , Wang Hailin , Tang Huini , Qiao Xiuqin , Feng Juanjuan , Wu Jianyong , Xing Chaozhu
Published: June 2020

Background

Utilization of heterosis has greatly improved the productivity of many crops worldwide. Understanding the potential molecular mechanism about how hybridization produces superior yield in upland cotton is critical for efficient breeding programs.

Results

In this study, high, medium, and low hybrids varying in the level of yield heterosis were screened based on field experimentation of different years and locations. Phenotypically, high hybrid produced a mean of 14% more seed cotton yield than its better parent. Whole-genome RNA sequencing of these hybrids and their four inbred parents was performed using different tissues of the squaring stage. Comparative transcriptomic differences in each hybrid parent triad revealed a higher percentage of differentially expressed genes (DEGs) in each tissue. Expression level dominance analysis identified majority of hybrids DEGs were biased towards parent like expressions. An array of DEGs involved in ATP and protein binding, membrane, cell wall, mitochondrion, and protein phosphorylation had more functional annotations in hybrids. Sugar metabolic and plant hormone signal transduction pathways were most enriched in each hybrid. Further, these two pathways had most mapped DEGs on known seed cotton yield QTLs. Integration of transcriptome, QTLs, and gene co-expression network analysis discovered genes Gh_A03G1024, Gh_D08G1440, Gh_A08G2210, Gh_A12G2183, Gh_D07G1312, Gh_D08G1467, Gh_A03G0889, Gh_A08G2199, and Gh_D05G0202 displayed a complex regulatory network of many interconnected genes. qRT-PCR of these DEGs was performed to ensure the accuracy of RNA-Seq data.

Conclusions

Through genome-wide comparative transcriptome analysis, the current study identified nine key genes and pathways associated with biological process of yield heterosis in upland cotton. Our results and data resources provide novel insights and will be useful for dissecting the molecular mechanism of yield heterosis in cotton
X-linked muscular dystrophy in a Labrador Retriever strain: phenotypic and molecular characterization.
Authors: Barthelemy Inès , Calmels Nadège , Weiss Robert B. , Tiret Laurent , Vulin Adeline , Wein Nicolas , Peccate Cécile , Drougard Carole , Beroud Christophe , Deburgrave Nathalie , Thibaud Jean-Laurent , Escriou Catherine , Punzon Isabel , Garcia Luis , Kaplan Jean-Claude , Flanigan Kevin M. , Leturcq France , Blot Stéphane
Published: June 2020

BackgroundCanine models of Duchenne muscular dystrophy (DMD) are a valuable tool to evaluate potential therapies because they faithfully reproduce the human disease. Several cases of dystrophinopathies have been described in canines, but the GRMD (Golden Retriever Muscular Dystrophy) model remains the most used in preclinical studies. Here we report a new spontaneous dystrophinopathy in a Labrador retriever strain, named LRMD (Labrador Retriever Muscular Dystrophy).MethodsA colony of LRMD dogs was established from spontaneous cases. Fourteen LRMD dogs were followed-up and compared to the GRMD standard using several functional tests. The disease causing mutation was studied by several molecular techniques and identified using RNA-sequencing.ResultsThe main clinical features of the GRMD disease were found in LRMD dogs; the functional tests provided data roughly overlapping with those measured in GRMD dogs, with similar inter-individual heterogeneity. The LRMD causal mutation was shown to be a 2.2‑Mb inversion disrupting the DMD gene within intron 20 and involving the TMEM47 gene. In skeletal muscle, the Dp71 isoform was ectopically expressed, probably as a consequence of the mutation. We found no evidence of polymorphism in either of the two described modifier genes LTBP4 and Jagged1. No differences were found in Pitpna mRNA expression levels that would explain the inter-individual variability.ConclusionsThis study provides a full comparative description of a new spontaneous canine model of dystrophinopathy, found to be phenotypically equivalent to the GRMD model. We report a novel large DNA mutation within the DMD gene and provide evidence that LRMD is a relevant model to pinpoint additional DMD modifier genes.
A comprehensive multivariate model of biopsychosocial factors associated with opioid misuse and use disorder in a 2017-2018 United States national survey
Authors: Ishino Francisco Alejandro Montiel , Gilreath Tamika , Salmeron Bonita , Williams Faustine
Published: June 2020

Background: Few studies have comprehensively and contextually examined the relationship of variables associated with opioid use. Our purpose was to fill a critical gap in comprehensive risk models of opioid misuse and use disorder in the United States by identifying the most salient predictors.Methods: A multivariate logistic regression was used on the 2017 and 2018 National Survey on Drug Use and Health, which included all 50 states and the District of Columbia of the United States. The sample included all noninstitutionalized civilian adults aged 18 and older (N=85,580; weighted N=248,008,986). The outcome of opioid misuse and/or use disorder was based on reported prescription pain reliever and/or heroin use dependence, abuse, or misuse. Biopsychosocial predictors of opioid misuse and use disorder in addition to sociodemographic characteristics and other substance dependence or abuse were examined in our comprehensive model. Biopsychosocial characteristics included socioecological and health indicators. Criminality was the socioecological indicator. Health indicators included self-reported health, private health insurance, psychological distress, and suicidality. Sociodemographic variables included age, sex/gender, race/ethnicity, sexual identity, education, residence, income, and employment status. Substance dependence or abuse included both licit and illicit substances (i.e., nicotine, alcohol, marijuana, cocaine, inhalants, methamphetamine, tranquilizers, stimulants, sedatives). Results. The comprehensive model found that criminality (adjusted odds ratio [AOR]=2.58, 95% confidence interval [CI]=1.98-3.37, p<0.001), self-reported health (i.e., excellent compared to fair/poor [AOR=3.71, 95%CI=2.19-6.29, p<0.001], good [AOR=3.43, 95%CI=2.20-5.34, p<0.001], and very good [AOR=2.75, 95%CI=1.90-3.98, p<0.001]), no private health insurance (AOR=2.12, 95%CI=1.55-2.89, p<0.001), serious psychological distress (AOR=2.12, 95%CI=1.55-2.89, p<0.001), suicidality (AOR=1.58, 95%CI=1.17-2.14, p=0.004), and other substance dependence or abuse were significant predictors of opioid misuse and/or use disorder. Substances associated were nicotine (AOR=3.01, 95%CI=2.30-3.93, p<0.001), alcohol (AOR=1.40, 95%CI=1.02-1.92, p=0.038), marijuana (AOR=2.24, 95%CI=1.40-3.58, p=0.001), cocaine (AOR=3.92, 95%CI=2.14-7.17, p<0.001), methamphetamine (AOR=3.32, 95%CI=1.96-5.64, p<0.001), tranquilizers (AOR=16.72, 95%CI=9.75-28.65, p<0.001), and stimulants (AOR=2.45, 95%CI=1.03-5.87, p=0.044). Conclusions. Biopsychosocial characteristics such as socioecological and health indicators, as well as other substance dependence or abuse were stronger predictors of opioid misuse and use disorder than sociodemographic characteristics.
Climatology of Medium-Scale Traveling Ionospheric Disturbances (MSTIDs) Observed with GPS Networks in the North African Region
Authors: Oluwadare Temitope Seun , Jakowski Norbert , Valladares Cesar E. , Akala Andrew Oke-Ovie , Abe Oladipo E. , Alizadeh Mahdi M. , Schuh Harald
Published: June 2020

We present for the first time the climatology of medium-scale traveling ionospheric disturbances (MSTIDs) by using Global Positioning System (GPS) receiver networks on geomagnetically quiet days (Kp ≤ 3) over the North African region during 2008-2016. The MSTIDs appear frequently as oscillating waves or wave-like structures in electron density induced by the passage of Atmospheric Gravity Waves (AGW) propagating through the neutral atmosphere and consequently, causing fluctuation in the ionospheric Total Electron Content (TEC). The TEC perturbations (dTEC) data are derived from dual frequency GPS-measurements. We have statistically analyzed the MSTIDs characteristics, occurrence rate, seasonal behavior as well as the interannual dependence. The results show a local and seasonal dependence of nighttime and daytime MSTIDs. The propagation direction is predominantly towards the South (equatorward), MSTIDs event period is (12 ≤ period ≤ 53 mins), and dominant amplitude (0.08 ≤ amp ≤ ~1.5 TECU), with a propagation velocity higher at daytime than nighttime. The amplitudes of the MSTIDs increase with solar activity. The local MSTIDs Spatio-temporal heat reveals variability in disturbance occurrence time, but seems to be dominant within the hours of (Northwest: 1200–1600 LT) and (Northeast: 1000–1400 LT) in December solstice during daytime, and around (NW: 2100–0200 LT) and (NE: 1900–0200 LT) in June solstice, but get extended to March equinox during solar maximum (2014) during the nighttime. The time series of MSTIDs regional distribution map is also generated. Atmospheric gravity waves (AGW) seems to be responsible for the daytime MSTIDs occurrence.
Prevalence and genotype distribution of HPV infection among 214,715 women from Southern China, 2012-2018: baseline measures prior to mass HPV vaccination
Authors: Luo Lipei , He Ping , liu qiaotu , jiang Yanghua , Zhang Yang-nan , Li Qingzhao , Li Qiu , Li Shengtao , Yang Fan , Ling Hua , Dai Xingui , Li Zhongyu , Chen Hongliang
Published: June 2020

Background: The epidemiology on the human papillomavirus (HPV) among females in Southern China is not well-established. Baseline data on the prevalence of HPV infection in China prior to mass prophylactic HPV vaccination would be useful. Thus, this study aimed to determine the type-specific HPV prevalence and distribution among females from Southern China prior to mass HPV vaccination. Methods: A retrospective cross-sectional study employing 214,715 women attending ChenZhou NO.1 People’s Hospital for cervical screening during 2012-2018 was conducted prior to widespread HPV vaccination. HPV genotype was detected using nucleic acid molecular diversion hybridization tests. The overall prevalence, age-specific prevalence, type distribution, and annual trend were analyzed. Results: The overall HPV prevalence was 18.71% (95% confidence interval [CI], 18.55%-18.88%) among Southern China females. During 2012–2018, the prevalence of HPV infection showed a downward tendency, from 21.63% (95% CI, 21.07%-22.20%) in 2012 to 18.75% (95% CI, 18.35%-19.16%) in 2018. Age-specific HPV distribution displayed a peak at young women aged less than 21 years (33.11%, 95% CI, 31.13%-35.15%), 20.07% (95% CI, 19.70%-20.44%) among women aged 21-30 years, 17.29% (95% CI, 17.01%-17.57%) among women aged 31-40 years, 17.23% (95% CI, 16.95%-17.51%) among women aged 41-50 years, 21.65% (95% CI, 21.11%-22.20%) among women aged 51-60 years, and 25.95% (95% CI, 24.86%-27.07%) among women aged over 60 years. Of the 21 subtypes identified, the most three prevalent high-risk HPV (HR-HPV) genotypes were HPV52 (5.12%; 95% CI, 21.11%-22.20%), -16 (2.96%; 95% CI, 2.89%-3.03%), and -58 (2.51%; 95% CI, 2.44%-2.58%); the predominant low-risk HPV (LR-HPV) genotypes were HPV81 (1.86%; 95%CI, 1.80%-1.92%) and -6 (0.69%; 95% CI, 0.66%-0.73%) respectively. Incidence of HR-HPV only, LR-HPV only and mixed LR- and HR-HPV were 15.17%, 2.07% and 1.47% respectively. Besides, single HPV infection accounted for 77.30% of all positive cases in this study.Conclusions: This study highlights 1) a high prevalence of HPV infection among females with a decreasing tendency towards 2012-2018, especially for young women under the age of 21 prior to mass HPV vaccination; 2) HPV52, -16 and -58 were the predominant HPV genotypes, suggesting potential use of HPV vaccine covering these HPV genotypes in Southern China.
Effects on applying Micro-Film Case-Based Learning model in pediatrics education
Authors: Pan Yuan , Chen Xiuqi , Wei Qiuwen , Zhao Jinmin , Chen Xun
Published: June 2020

Background: In view of the harsh reality Chinese paediatricians face, the challenge of paediatric education is about instilling not only knowledge and clinical skills but also resilience and beliefs. The aim of the study is to explore a more effective method than the traditional lecture-based learning (LBL) model for optimizing educational outcomes by establishing an innovative, comprehensive, case-based learning (CBL) model combined with the micro-film technique (MF+CBL). This approach has four important components: interests (attraction), knowledge application, competency, and scenario coping skills. Methods: Experimental research was conducted via a controlled parallel group study. The total sample of 104 senior-year students (Chinese) majoring in clinical medicine was randomly divided into two groups. The experimental group was exposed to the MF+CBL model and the control group to the LBL model. Overall, the results were assessed after an 8-week course via a student self-assessment questionnaire, a satisfaction survey and the final examination.Results: The experimental group generally performed better than the control group on the student self-assessment (P＜0.05), satisfaction survey (P＜0.05), and final examination (80.02±3.77 vs 73.65±3.69, P = 0.000). The open question at the end of the questionnaire revealed that a small number of students did not favour the MF+CBL model due to its time- and energy-consuming features.Conclusions: Compared with LBL, the MF+CBL model was an innovative teaching method that promoted more comprehensive quality development. It represents an alternative model for optimizing the capacity of future paediatric doctors.
Clinical application of chromosomal microarray analysis for fetuses with craniofacial malformations
Authors: Xu Chenyang , Xiang Yanbao , Xu Xueqin , Zhou Lili , Li Huanzheng , Dong Xueqin , Tang Shaohua
Published: June 2020

Background: The potential correlations between chromosomal abnormalities and craniofacial malformations (CFMs) remain a challenge in prenatal diagnosis. This study aimed to evaluate 118 fetuses with CFMs by applying chromosomal microarray analysis (CMA) and G-banded chromosome analysis. Results: Of the 118 cases in this study, 39.8% were isolated CFMs (47/118) whereas 60.2% were non-isolated CFMs (71/118). The detection rate of chromosomal abnormalities in non-isolated CFM fetuses was significantly higher than that in isolated CFM fetuses (26/71 vs. 7/47, p = 0.01). Compared to the 16 fetuses (16/104; 15.4%) with pathogenic chromosomal abnormalities detected by karyotype analysis, CMA identified a total of 33 fetuses (33/118; 28.0%) with clinically significant findings. These 33 fetuses included cases with aneuploidy abnormalities (14/118; 11.9%), microdeletion/microduplication syndromes (9/118; 7.6%), and other pathogenic copy number variations (CNVs) only (10/118; 8.5%).We further explored the CNV/phenotype correlation and found a series of clear or suspected dosage-sensitive CFM genes including TBX1, MAPK1, PCYT1A, DLG1, LHX1, SHH, SF3B4, FOXC1, ZIC2, CREBBP, SNRPB, and CSNK2A1.Conclusion: These findings enrich our understanding of the potential causative CNVs and genes in CFMs. Identification of the genetic basis of CFMs contributes to our understanding of their pathogenesis and allows detailed genetic counselling.
Downregulation of Dickkopf-1 Augments the Therapeutic Effects of Endometrial Regenerative Cells on Experimental Colitis
Authors: Yu Dingding , Zhao Yiming , Hu Yonghao , Kong Dejun , Jin Wang , Qin Yafei , Zhang Baoren , Li Xiang , Hao Jingpeng , Wang Hongda , Li Guangming , Wang Hao
Published: June 2020

Background

We have demonstrated that endometrial regenerative cells (ERCs) are mesenchymal-like stromal cells and can attenuate experimental colitis, however, its underlying mechanism needs further investigation. Dickkopf-1 (DKK1), a glucoprotein secreted by mesenchymal stromal cells (MSCs), is a classical inhibitor of Wnt/β-catenin pathway which is closely associated with the development of colitis. Therefore, the objective of this study was to investigate whether ERCs could also secret DKK1, and whether the downregulation of DKK1 (DKK1 low -ERCs) would enhance the therapeutic effects of ERCs in attenuation of experimental colitis.

Methods

BALB/c mice were given 3% dextran sodium sulfate (DSS) for 7 consecutive days and free tap water for 3 days sequentially to induce experimental colitis. Unmodified ERCs, IL-1β-treated ERCs (DKK1 low -ERCs) and glucocorticoid-treated ERCs (DKK1 high -ERCs) were injected (1 million/mouse/day, i.v. ) on day 2, 5 and 8 respectively. Colonic and splenic samples were harvested on day 10 after DSS-induction.

Results

It was found that DKK1 low -ERC treatment markedly attenuated colonic damage, body weight loss and colon-length shortening in colitis mice. Compared with other treatments, cell populations of CD4 + IL-4 + Th2, CD4 + CD25 + FOXP3 + Treg, and CD68 + CD206 + macrophages in spleens were also significantly upregulated in DKK1 low -ERC group ( p < 0.05). In addition, lower expression of pro-inflammatory (TNF-α and IFN-γ), but higher levels of anti-inflammatory cytokines (IL-4 and IL-10) and β-catenin were detected in colons in DKK1 low -ERC group ( p < 0.01 vs. other groups).

Conclusions

DKK1 low -ERCs display augmented immunoregulatory ability and therapeutic effects in DSS-induced colitis.
Clinical features and outcome of fecal retention in infancy
Authors: Ren Lu , Geng Lan-Lan , Wang hong-li , Gong si-tang , Xie Jing , Xiong Li-Ya , Chen Pei-Yu
Published: June 2020

1) Background: To understand the clinical features and outcome of fecal retention in infancy(FRI), so as to guide the focus and intervention methods of such infants.2) Methods: The electronic medical record system(EMRS)was used to collect and screen out cases diagnosed as fecal retention from June 2018 to June 2019 in outpatient clinic of our hospital. The age, feeding method, frequency and traits of stool, and accompanying symptoms were recorded. The changes of clinical symptoms, medical examinations and drug treatment by the age of 1 year were investigated by means of electronic medical record review and telephone follow-up.3) Results: A total of 286 infants were enrolled, 7 were lost to follow-up, and 279 were effectively followed up. There were 157 males and 129 females, with an age of 3.6 ± 1.5 months. The average stool frequency was 5.9 ± 1.8 days. 63.3% of the infants were breast-fed, 16.8% were formula-fed, and the rest were mixed-fed,all without supplementary food. 9.1% of the infants showed corresponding gastrointestinal symptoms, such as bloating, increased crying, decreased milk intake, and laborious defecation. 87.1% of the infants received medical treatment, including glycerin enema, probiotics, and Chinese herbal preparations, with an effective treatment rate of 7.8%. 38.7% of infants have undergone medical examination, including abdominal ultrasound, X-ray film / barium enema, blood test, etc, the positive rate is 14.8%. The duration of fecal retention in 53% of infants was ≤ 2 months, 22.6% between 2–3 months, 24.4% ≥ 3 months, with an average of 2.6 ± 1.1 months. At the age of followed up to 1 year, 16.8% of infants developed functional constipation(FC). Compared with other infants with normal defecation, there was no significant difference in age, frequency of stool, the proportion of breast milk feeding and receiving treatment, and there was a significant difference in the duration of fecal retention. The duration of FC group was longer than normal defecation group by which was 3.49 ± 0.83 months.4) Conclusions: Infants with fecal retention are more likely to develop FC at age 1 than general population, and may be positively related to the duration of fecal retention.
Predictors of Patient Satisfaction; Quality Dimensions and Demographic Characteristics
Authors: Farrokhi Pouria , Hashjin Asgar Aghaei , Bagherzadeh Rafat , Zarei Ehsan
Published: June 2020

Background: The healthcare industry is increasingly growing in a competitive atmosphere. One of the essential key issues for the survival of healthcare organizations is patient satisfaction. This study aimed to investigate the impact of health service quality and demographic characteristics on patient satisfaction with outpatient departments at teaching hospitals affiliated with Tehran University of Medical Sciences in Iran.Method: This cross-sectional study was conducted in 2018. A sample of 400 patientsreferring to outpatient departments at teaching hospitalswasrandomly selected. A valid and reliable questionnaire was used to collect data which were then analyzed by using SPSS 23. Results: The mean scores of service quality and patient satisfaction were 3.73 ± 0.51 and 3.61 ± 0.97 out of 5, respectively. Moreover, patients’ demographic characteristics; age, marital status, residence area, and service quality dimensions; admission process, physician consultation, service costs, accessibility, and appointment were identified as the most effectivefactors on patient satisfaction. Conclusion: In order to increase patient satisfaction, physicians and reception staff are advised to provide patients with useful information and cost-effective services. Nonetheless,it seems necessary for teaching hospitalsto establish plans which facilitate payment, appointment, and examination process.
Arthroscopic Cartilage Regeneration Facilitating Procedure: A Decompressing Arthroplasty for Knee Osteoarthritis
Authors: Lyu Shaw-Ruey , Hung Jung-pin , Hsu Chia-Chen , Chen Yu-Ruei , Lin Chih-Wen
Published: June 2020

Background The effectiveness of arthroscopic treatment for knee osteoarthritis (OA) has been subject to debate. This study presents an innovative concept of arthroscopic management for knee OA and investigates its clinical outcome.Methods An arthroscopic cartilage regeneration facilitating procedure (ACRFP) to eliminate medial abrasion phenomenon and decompress the patello-femoral joints was performed on 693 knees of 411 patients with knee OA, mean age 60 years (34-90). The Knee Society score (KSS) and the knee injury and osteoarthritis outcome score (KOOS) were used for subjective outcome study. Roentgenographic changes for all cases and magnetic resonance imaging (MRI) variations for twenty randomly selected cases were evaluated for objective outcomes. Results There were 634 knees in 369 patients (93.7%) available with more than 3 years of follow-ups (mean 40 months, SD 9). The overall subjective satisfactory rate was 91.1%. The KSS and all subscales of the KOOS improved statistically. Reversal of the degeneration process of cartilage was observed in 80.1% of the whole series (radiographic outcome) and in 72.2% of the 18 randomly selected cases (one-year MRI outcome study). Gender and OA severity were related to subjective outcomes. Age, body mass index (BMI), pre-operative hyaluronic acid injection, OA severity, and the type and severity of the medial plica are important predictors of radiographic outcomes. An analysis of failed cases reaffirmed the need for early ACRFP and skillful post-operative care. Conclusion ACRFP is an effective treatment for knee OA. If performed in time, it can satisfy most patients and might modify their degeneration process.
Lipidomic Profiling Reveals Distinct Differences in Plasma Lipid Composition in Overweight or Obesity Adolescence Student
Authors: Yang Longyan , Yin Ruili , Wang Xiaojing , Yu Ke
Published: June 2020

Introduction：The relationship between dyslipidemia and obesity has been widely reported, but the global lipid profiles associated with the development of obesity remain to be clarified, especially in the East Asia teenage population. Methods：Mass spectrometry coupled with liquid chromatography was applied to detect the global lipidome in the fasting plasma from 90 Chinese adolescence, including 30 obesity adolescents, 30 overweight adolescents, and 30 adolescents with normal body mass index (BMI). All participants were performed anthropometric measurement by using InBody 770. Clinical biochemical indicators were measured by Cobas Elecsys 601.Results：Both qualitative and quantitative analyses revealed a gradual change in plasma lipid features with obesity students exhibiting characteristics close to overweight students, but they differed significantly from students with normal. The levels of triglyceride (TG), 18-Hydroxycortisol, Isohumulinone A, and 11-Dihydro-12-norneoquassin were up-regulated in obesity group, while phosphatidylcholine (PC), phosphatidylethanolamine (PE), LysoPC, LysoPE, and Phosphatidylinositol (PI) were significantly down-regulated in obesity group than in control and overweight individuals. Then conducted venn diagram and selected 8 significant metabolites from the 3 paired comparisons. Most of the selected features significantly correlated with anthropometric measurement.Conclusions: The altered plasma lipidome in Chinese obesity and overweight students suggests that lipid features may play important role in the pathogenesis of obesity and that such features may provide a basis for evaluating risk and monitoring obesity development.
Effects of alendronate in bone repair: A study in rat femur fractures treated with plates and screws
Authors: Tiboni Fernanda , Weiss Suyany Gabriely , Gerber Jennifer Tsi , Michels Arieli Carini , Rosa Thais Grupp da , Casagrande Thaís Costa , Deliberador Tatiana Miranda , Giovanini Allan Fernando , Johann Aline Cristina , Scariot Rafaela
Published: June 2020

Background

Alendronate (ALN) has direct action on bone metabolism, increasing osteogenesis and decreasing bone resorption. The study rated the effect of ALN on femoral fracture repair and the effect of different doses of the drug on the liver and kidneys.
Methods

Wistar rats were divided into groups: A1 (ALN 1 mg/kg), A2 (ALN 3 mg/kg), and C (saline solution). The drug/solution was applied intraperitoneally three times a week after femoral fracture until euthanasia 45 days later.
Results

Liver analysis from group A1 presented normal histological aspects, while hepatic steatosis was observed in group A2. In groups A1 and A2, kidneys showed amylocymile like cell degeneration. In femur bone callus, no difference was observed in collagens I and III or in number of trabeculae (p ≥ 0.05). Immunohistochemical evaluation showed positivity for the Transforming Grow Factor β-1 (TGFβ-1) marker in the control group, in spinal area and in small chondrocytes, but negativity for hypertrophy. In A1, an extensive area of cartilaginous expansion was observed, with positive hypertrophic TGFβ-1 cartilage, even in areas with bone matrix. A low positivity was observed in the medullar area, in contrast to the control. Group A2 presented a high number of chondroid matrices and a moderate number of TGFβ-1 cells, with little positivity in medullary area.
Conclusions

A dosage of ALN 1 mg/kg promotes cellular differentiation activity in the bone callus region, with mild damage in the liver and kidneys. A dosage of ALN 3 mg/kg became toxic without positive alterations in cell differentiation.
Tissue-nonspecific Alkaline Phosphatase Blockade Ameliorates Behavioural Deficits and Life-expectancy on Tauopathies
Authors: Sebastián-Serrano Álvaro , Merchán-Rubira Jesús , Di-Lauro Caterina , Bianchi Carolina , Narisawa Sonoko , Millan Jose Luis , Avila Jesus , Hernandez Felix , Diaz-hernandez Miguel
Published: June 2020

The authors have withdrawn this preprint due to erroneous posting.
Transjugular Intrahepatic Portosystemic Shunt for Hepatic Sinusoidal Obstruction Syndrome Associated with Consumption of Gynura Segetum
Authors: Zhang Lijie , Li Qing , Makamure Joyman , Zhao Dan , Liu Ziyi , Zheng Chuansheng , Liang Bin
Published: June 2020

Background: To evaluate the efficacy and safety of transjugular intrahepatic portosystemic shunt (TIPS) on hepatic sinusoidal obstruction syndrome (HSOS) associated with consumption of Gynura segetum (GS).Methods: We retrospectively reviewed 9 consecutive patients with GS-related HSOS who were refractory to supportive treatment and underwent TIPS at our institution between January 2014 and September 2019. The patients were evaluated for safety and efficacy, including TIPS complications and changes in portosystemic pressure gradient (PPG), ascites, total bilirubin, liver size and portal vein diameter.Results: TIPS procedures were performed successfully in the 9 patients, without any TIPS technique-related complications. The PPG was improved by TIPS in all patients (mean PPG before TIPS, 30.4 ± 5.2 vs. 13.0 ± 4.1 mm Hg post-TIPS, P=0.008). Except for one patient who was lost to follow-up, the remaining 8 patients survived with a median follow-up period of 12 months (range, 5-39 months). Although the level of total bilirubin was significantly increased 5-7 days after TIPS compared with that before the procedure (3.57 ± 1.58 vs. 4.82 ± 2.06 mg/dl, P=0.017), it returned to baseline level at 1-month follow-up (3.53 ± 2.72 vs. 4.82 ± 2.06 mg/dl, P=0.401). The patients experienced complete resolution or obvious reduction of ascites (P<0.001), significant reduction of liver size (16.7 ± 2.2 vs. 13.7 ± 1.7 cm, P=0.018), and significant enlargement of the portal trunk (10.7 ± 2.5 vs. 13.4 ± 2.4 mm, P=0.017) after TIPS compared to the pre-TIPS state.Conclusion: TIPS may offer a potentially useful treatment for the GS-related HSOS.
Transient Heat-Shock Disrupts Molecular Signals and Competence of Riverine Bubalus Bubalis Oocytes and Early Embryos in a Stage-Specific Manner
Authors: Singh Devender , Kumar Manish , Kumar Rakesh , Datta Tirtha Kumar
Published: June 2020

Background Thermal stress elicits detrimental effect in reproduction performance of Bubalus bubalis, buffalo. Riverine buffalo oocytes and early embryos possess differential ability to grow and survive under thermal stress condition. It is interesting to know how precisely the short and transient heat stress impacts growth and development of buffalo oocytes and early embryos in a stage specific manner. In this study, we aim to identify the most sensitive and vulnerable stage of oocytes and early embryos against transient heat stress as well as unravel the underlying molecular signals responsible for the stunted embryo development under thermal stress. It was assessed by utilizing six different groups of oocytes and embryos with an incorporation of 4 h transient heat shock at 40ºC during different stage of in-vitro maturation (IVM) and in vitro culture (IVC). Results The stressed oocytes of group (grp) 1, 2 and 3 exhibit comparable paces of attaining metaphase-II (M-II) phase at different time interval of IVM. The most detrimental effect of heat stress was observed in grp 2 with sharp reduction in morula to blastocysts transition rate (p<0.05). Expression of mRNA transcripts of HSP8, MnSOD and Sirt-3 genes were significantly increased from mid-IVM to 4-cell embryo although subsequently, down-regulated in embryos of 8-16 cells, morulae and blastocysts. Expression of maternal to embryo transition (MET) genes viz. PAP, U2Af and eIF4A was significantly down regulated from 2-cell embryo to morula (p<0.05). Maternal recognition of pregnancy (MRP) and morulae to blastocyst transition genes were poorly expressed in grp 2 than of the other stress groups (p<0.05). Conclusions Disruption of molecular signal has implicated in the poor formation of inner cell mass (ICM) and trophectoderm (TE) cells, this results in compromised development prospects of early buffalo embryos in a stage specific manner. Well-coordinated molecular signals associated with heat stress held responsible for reduced development of early embryo. We establish the most vulnerable stage of buffalo oocytes and presumptive zygotes against transient heat shock and observe a narrow window as the most critical stage for regulating the development potential of the prospective embryos under short heat stress environment.