bluuebunny/crossref_metadata_2025 huggingface dataset:
Revealing the distinct clinical patterns and relapse risk factors in seronegative IgG4‐RD patients: A retrospective cohort study over a decade
Authors: Peng Yu , Wang Mu , Sun Ruijie , Nie Yuxue , Zhang Nianyi , He Xin , Sun Boyuan , Peng Linyi , Fei Yunyun , Zhou Jiaxin , Li Mengtao , Zhang Wen
Published: June 2024

Objectives

Our study aimed to investigate the distinct clinical patterns of seronegative IgG4‐related disease (IgG4‐RD) patients.

Methods

We retrospectively enrolled 698 treatment‐naïve IgG4‐RD patients in this study. Patients were divided into four different subgroups according to their baseline serum IgG4 levels. The distinct clinical patterns of seronegative IgG4‐RD patients were revealed through the comparison of baseline clinical data and disease prognosis among the different subgroups. COX regression analyses were used to investigate the risk factors for disease relapse and to construct the nomogram model.

Results

Seronegative IgG4‐RD patients account for a minority of IgG4‐RD patients (49/698, 7.02%). The proportions of seronegative IgG‐RD patients in our study and several Asian cohorts were significantly lower than those of the European and American cohorts. Seronegative IgG4‐RD patients got lower serum IgG levels (p < 0.0001), lower eosinophil count (p < 0.0001), lower serum IgE levels (p < 0.0001)), lower IgG4‐RD responder index (RI) scores (p < 0.0001), and fewer affected organ numbers (p < 0.0001) compared with other subgroups, whereas they were more likely to manifest fibrotic type with some special organ involvement. Younger age at onset, GCs monotherapy, elevated C‐reactive protein level, and elevated erythrocyte sedimentation rate level are the risk factors for the disease relapse of seronegative IgG4‐RD patients. An effective nomogram model predicting disease relapse of seronegative IgG4‐RD patients was constructed. Seronegative IgG4‐RD patients with scores >84.65 at baseline were susceptible to suffering from disease relapse.

Conclusions

Distinct clinical features and multiple risk factors for disease relapse of seronegative IgG4‐RD patients have been revealed in this study. A nomogram model was constructed to effectively predict disease relapse during the follow‐up period.
Mitochondrial puzzle in muscle: Linking the electron transport system to overweight
Authors: Casuso Rafael A.
Published: June 2024

Human skeletal muscle mitochondria regulate energy expenditure. Research has shown that the functionality of muscle mitochondria is altered in subjects with overweight, as well as in response to nutrient excess and calorie restriction. Two metabolic features of obesity and overweight are (1) incomplete muscular fatty acid oxidation and (2) increased circulating lactate levels. In this study, I propose that these metabolic disturbances may originate from a common source within the muscle mitochondrial electron transport system. Specifically, a reorganization of the supramolecular structure of the electron transport chain could facilitate the maintenance of readily accessible coenzyme Q pools, which are essential for metabolizing lipid substrates. This approach is expected to maintain effective electron transfer, provided that there is sufficient complex III to support the Q‐cycle. Such an adaptation could enhance fatty acid oxidation and prevent mitochondrial overload, thereby reducing lactate production. These insights advance our understanding of the molecular mechanisms underpinning metabolic dysregulation in overweight states. This provides a basis for targeted interventions in the quest for metabolic health.
Breaking up male‐dominated collusive arrangements: Women's representation in bureaucracy and corruption
Authors: Cho Beomgeun , Kim Heeun
Published: June 2024

We investigate the impact of women's representation in bureaucracy on corruption. In this study, we demonstrate how women bureaucrats' experiences in male‐dominated workplaces contribute to curbing corruption. To do this, we investigate three dimensions of women's representation (average share, average rank, and hierarchical dispersion) in South Korean regional governments. Empirical evidence shows that the higher women bureaucrats are ranked, the more bureaucrats are disciplined against misbehaviors and the lower the corruption risks are in public procurement. The findings imply that having women bureaucrats at higher ranks reduces corruption risks because they disrupt male‐dominated collusive arrangements and abide by ethical standards to legitimize their leadership.
Biosynthesis and differential spatial distribution of the 3‐deoxyanthocyanidins apigenidin and luteolinidin at the interface of a plant‐cyanobacteria symbiosis exposed to cold
Authors: Güngör Erbil , Bartels Benjamin , Bolchi Giorgio , Heeren Ron M. A. , Ellis Shane R. , Schluepmann Henriette
Published: June 2024

Aquatic ferns of the genus Azolla (Azolla) form highly productive symbioses with filamentous cyanobacteria fixing N2 in their leaf cavities, Nostoc azollae. Stressed symbioses characteristically turn red due to 3‐deoxyanthocyanidin (DA) accumulation, rare in angiosperms and of unknown function. To understand DA accumulation upon cold acclimation and recovery, we integrated laser‐desorption‐ionization mass‐spectrometry‐imaging (LDI‐MSI), a new Azolla filiculoides genome‐assembly and annotation, and dual RNA‐sequencing into phenotypic analyses of the symbioses. Azolla sp. Anzali recovered even when cold‐induced DA‐accumulation was inhibited by abscisic acid. Cyanobacterial filaments generally disappeared upon cold acclimation and Nostoc azollae transcript profiles were unlike those of resting stages formed in cold‐resistant sporocarps, yet filaments re‐appeared in leaf cavities of newly formed green fronds upon cold‐recovery. The high transcript accumulation upon cold acclimation of AfDFR1 encoding a flavanone 4‐reductase active in vitro suggested that the enzyme of the first step in the DA‐pathway may regulate accumulation of DAs in different tissues. However, LDI‐MSI highlighted the necessity to describe metabolite accumulation beyond class assignments as individual DA and caffeoylquinic acid metabolites accumulated differentially. For example, luteolinidin accumulated in epithelial cells, including those lining the leaf cavity, supporting a role for the former in the symbiotic interaction during cold acclimation.
An innovative referral system: Bridging healthcare providers and community supports for pregnant and parenting people
Authors: Hirst Kimberly , Fixsen Amanda A. M.
Published: June 2024

The connection between community healthcare providers and available community‐based programs is often weak. For community members to be connected with services, a strong referral link between providers and programs is paramount. The Referral Express and Central Hub (REACH) project was born out of this need for Nurse–Family Partnership (NFP) program sites in the Denver Metro area of Colorado to be better connected and coordinated with local referral sources. The objective of the REACH pilot was to create a centralized referral system and determine its feasibility in practice. After a 12‐month pilot, this innovative referral system supported the increase in a number of referral partners to Denver Metro area sites, an increase in clients served across the Denver Metro area, as well as an increase in the “refer to enroll” rate which increased by 37%. The success of the pilot of REACH led the developers to test the process on a second program, Child First, which was also successful. REACH has proved to be sustainable and continues to operate for both programs. Additionally, REACH acts as a consultant to NFP sites outside the Denver Metro area, in the state of Colorado, as each site works to increase its relationships with referral partners.
Carrier‐based delivery system of phytohormones in plants: stepping outside of the ordinary
Authors: Tiwari Kavita , Tripathi Sneha , Mahra Shivani , Mathew Sobhitha , Rana Shweta , Tripathi Durgesh Kumar , Sharma Shivesh
Published: June 2024

Climate change is increasing the stresses on crops, resulting in reduced productivity and further augmenting global food security issues. The dynamic climatic conditions are a severe threat to the sustainability of the ecosystems. The role of technology in enhancing agricultural produce with the minimum environmental impact is hence crucial. Active molecule/Plant growth regulators (PGRs) are molecules helping plants' growth, development, and tolerance to abiotic and biotic stresses. However, their degradation, leaching in surrounding soil and ground water, as well as the assessment of the correct dose of application etc., are some of the technical disadvantages faced. They can be resolved by encapsulation/loading of PGRs on polymer matrices. Micro/nanoencapsulation is a revolutionary tool to deliver bioactive compounds in an economically affordable and environmentally friendly way. Carrier‐based smart delivery systems could be a better alternative to PGRs application in the agriculture field than conventional methods (e.g., spraying). The physiochemical properties and release kinetics of PGRs from the encapsulating system are being explored. Therefore, the present review emphasizes the current status of PGRs encapsulation approach and their potential benefits to plants. This review also addressed the mechanistic action of carrier‐based delivery systems for release, which may aid in developing smart delivery systems with specific tailored properties in future research.
Phenotyping revealed tolerance traits and genotypes for acidity and aluminum toxicity in European Vicia faba L
Authors: Belachew Kiflemariam Y. , Skovbjerg Cathrine Kiel , Andersen Stig U. , Stoddard Frederick L.
Published: June 2024

Soil acidity is a global issue; soils with pH <4.5 are widespread in Europe. This acidity adversely affects nutrient availability to plants; pH levels <5.0 lead to aluminum (Al3+) toxicity, a significant problem that hinders root growth and nutrient uptake in faba bean (Vicia faba L.) and its symbiotic relationship with Rhizobium. However, little is known about the specific traits and tolerant genotypes among the European faba beans. This study aimed to identify response traits associated with tolerance to root zone acidity and Al3+ toxicity and potentially tolerant genotypes for future breeding efforts. Germplasm survey was conducted using 165 genotypes in a greenhouse aquaponics system. Data on the root and shoot systems were collected. Subsequently, 12 genotypes were selected for further phenotyping in peat medium, where data on physiological and morphological parameters were recorded along with biochemical responses in four selected genotypes. In the germplasm survey, about 30% of genotypes showed tolerance to acidity and approximately 10% exhibited tolerance to Al3+, while 7% showed tolerance to both. The phenotyping experiment indicated diverse morphological and physiological responses among treatments and genotypes. Acid and Al3+ increased proline concentration. Interaction between genotype and environment was observed for ascorbate peroxidase activity, malondialdehyde, and proline concentrations. Genomic markers associated with acidity and acid+Al3+‐toxicity tolerances were identified using GWAS analysis. Four faba bean genotypes with varying levels of tolerance to acidity and Al3+ toxicity were identified.
Diagnostic methods/parameters to monitor peri‐implant conditions
Authors: Monje Alberto , Salvi Giovanni E.
Published: June 2024

The diagnostic accuracy of clinical parameters, including visual inspection and probing to monitor peri‐implant conditions, has been regarded with skepticism. Scientific evidence pointed out that primary diagnostic tools (chairside) seem to be highly specific, while their sensitivity is lower compared with their use in monitoring periodontal stability. Nonetheless, given the association between pocket depth at teeth and implant sites and the aerobic/anaerobic nature of the microbiome, it seems plausible for pocket probing depth to be indicative of disease progression or tissue stability. In addition, understanding the inflammatory nature of peri‐implant diseases, it seems reasonable to advocate that bleeding, erythema, ulceration, and suppuration might be reliable indicators of pathology. Nevertheless, single spots of bleeding on probing may not reflect peri‐implant disease, since implants are prone to exhibit bleeding related to probing force. On the other side, bleeding in smokers lacks sensitivity owing to the decreased angiogenic activity. Hence, the use of dichotomous scales on bleeding in the general population, in contrast to indices that feature profuseness and time after probing, might lead to false positive diagnoses. The definitive distinction between peri‐implant mucositis and peri‐implantitis, though, relies upon the radiographic evidence of progressive bone loss that can be assessed by means of two‐ and three‐dimensional methods. Accordingly, the objective of this review is to evaluate the existing clinical and radiographic parameters/methods to monitor peri‐implant conditions.
Lessons learned from CMS's National Partnership to Improve Dementia Care: a thematic synthesis of multiple stakeholder‐engaged studies
Authors: Winter Jonathan D. , Kerns J. William , Winter Katherine M. , Winter Christopher , Krist Alex , Etz Rebecca S.
Published: June 2024

Background

Antipsychotic prescribing in United States nursing homes (NHs) has decreased since the Center for Medicare & Medicaid Service debuted the National Partnership to Improve Dementia Care in Nursing Homes (NP); however, reductions have stalled. To help explain persistent antipsychotic use despite the NP's reduction efforts, the perspectives of diverse NP stakeholders were qualitatively assessed. This study aimed to re‐evaluate these individual perspectives in combined thematic synthesis to discover NP improvement opportunities undetectable in single stakeholder assessments.

Methods

Thematic synthesis. Through immersive crystallisation, original source coding results were organised into related descriptive themes. Similarities and differences were identified, and descriptive themes were regrouped into new, increasingly abstract, analytical themes. This cycle continued until variances were resolved and analytic themes sufficiently described and explained all initial descriptive themes.

Results

Three analytic themes emerged regarding NP improvement opportunities. The NP's positive impacts would be augmented by: (i) a deeper and expanded appreciation of stakeholder perspectives; (ii) more urgent and rapid adaptation to unintended adverse outcomes; and (iii) greater recognition of the contextual and environmental factors influencing decisions to prescribe or not prescribe antipsychotic medications. Stakeholder groups described: perspectives they perceived as inadequately considered by the NP; insufficient NP engagement with the stakeholders capable of creating evidenced, affordable, and available non‐pharmacologic therapies for dementia symptoms; recognition that dementia interventions effective for a specific individual at a specific time in a specific community may not generalise; and diverse ongoing undesirable outcomes from NP policies that could be mitigated by NP modifications.

Conclusions

The NP has done much to advance dementia care in NHs. Notwithstanding, these results suggest the NP would only be improved through increasingly comprehensive inclusion of stakeholder perspectives, enhanced incorporation of individual contextual factors, and a more decisive mechanism for ongoing and continual adaptation.
Computed tomography mucus plugs and airway tree structure in patients with chronic obstructive pulmonary disease: Associations with airflow limitation, health‐related independence and mortality
Authors: Tanabe Naoya , Shimizu Kaoruko , Shima Hiroshi , Wakazono Nobuyasu , Shiraishi Yusuke , Terada Kunihiko , Terada Satoru , Oguma Tsuyoshi , Sakamoto Ryo , Suzuki Masaru , Makita Hironi , Sato Atsuyasu , Sato Susumu , Nishimura Masaharu , Konno Satoshi , Hirai Toyohiro
Published: June 2024

Background and Objective

Mucus plugs and underlying airway tree structure can affect airflow limitation and prognosis in patients with chronic obstructive pulmonary disease (COPD), but their relative roles are unclear. This study used two COPD cohorts to examine whether mucus plugs on computed tomography (CT) were associated with airflow limitation and clinical outcomes independent of other airway structural changes and emphysema.

Methods

Based on visual CT assessment, patients with mucus plugs in 0, 1–2 and ≥3 lung segments were assigned to no‐, low‐ and high‐mucus groups. Loss of health‐related independence and mortality were prospectively recorded for 3 and 10 years in the Kyoto–Himeji and Hokkaido cohorts, respectively. The percentages of the wall area of the central airways (WA%), total airway count (TAC) and emphysema were quantified on CT.

Results

Of 199 and 96 patients in the Kyoto–Himeji and Hokkaido cohorts, 34% and 30%, respectively, had high mucus scores. In both cohorts, TAC was lower in the high‐mucus group than in the no‐mucus group, whereas their emphysema severity did not differ. High mucus score and low TAC were independently associated with airflow limitation after adjustment for WA% and emphysema. In multivariable models adjusted for WA% and emphysema, TAC, rather than mucus score, was associated with a greater rate of loss of independence, whereas high mucus score, rather than TAC, was associated with increased mortality.

Conclusion

Mucus plugs and lower airway branch count on CT had distinct roles in airflow limitation, health‐related independence and mortality in patients with COPD.

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In severe acne vulgaris, TNF‐α gene variants are connected to increased TNF‐α gene expression and insulin resistance
Authors: AbdElneam Ahmed Ibrahim , Alhajlah Sharif , Al‐Dhubaibi Mohammed Saleh , Bahaj Saleh Salem , Mohammed Ghada Farouk , Alantry Ahmed Kaid , Atef Lina Mohammed
Published: June 2024

Background

Acne vulgaris (AV) is a chronic inflammatory skin condition affecting the pilosebaceous unit, commonly presenting as comedones, papules, pustules, or nodules on the face, upper limbs, torso, and back, with comedones formation being the primary pathology leading to disfiguring inflammation, hyperpigmentation, scarring, and psychological impact.

Aim

The purpose of this study was to investigate the significance of two genetic variants in the promoter region of the tumor necrosis factor‐alpha (TNF‐α) gene and their association with insulin resistance (IR) in acne patients. To understand how these variants contribute to AV and its associated IR.

Subjects and methods

An analytical cross‐sectional study with a case‐control design and research evaluation was carried out on 87 AV patients and 73 healthy volunteers. The medical histories of both groups were obtained, as well as the severity and duration of inflammation among acne sufferers, as well as demographic data. Biochemical analysis was performed on both sets of participants, including fasting blood glucose levels, insulin levels while fasting, IR, and serum TNF‐α. PCR‐RFLP analysis identified −863 G > A (rs1800630) and −308 G > A (rs1800629) variations, and real‐time PCR analysis evaluated TNF‐α gene expression in both patients and healthy people.

Results

Acne patients exhibited significantly higher levels of IR, fasting glucose, fasting insulin, serum TNF‐α, and TNF‐α folding change, when compared to healthy controls. The co‐dominant model for −863 G > A and −308 G > A variants exhibited significant variations between the two groups. Severe acne patients who had the A/A genotype for −308 variants exhibited higher levels of IR, serum TNF‐α, and TNF‐α folding change. Highly significant positive linear correlation between IR, serum TNF‐α, and TNF‐α folding change in severe AV.

Conclusion

There is a correlation between AV, especially severe acne, and the −863 G > A and −308 G > A polymorphism, which influences TNF‐α gene expression and serum TNF‐α levels.
Identification of novel disulfidptosis‐related lncRNA signatures to predict the prognosis and immune microenvironment of skin cutaneous melanoma patients
Authors: Cheng Shengrong , Wang Xin , Yang Shuhan , Liang Jiahui , Song Caiying , Zhu Qiuxuan , Chen Wendong , Ren Zhiyao , Zhu Fei
Published: June 2024

Background

Skin cutaneous melanoma (SKCM) is an aggressive form of malignant melanoma with poor prognosis and high mortality rates. Disulfidptosis is a newly discovered cell death regulatory mechanism caused by the abnormal accumulation of disulfides. This unique pathway is guiding significant new research to understand cancer progression for targeted treatment. However, the correlation between disulfidptosis with long non‐coding RNAs (lncRNAs) in SKCM remains unknown at present.

Methods

The Cancer Genome Atlas database furnished lncRNA expression data and clinical information for SKCM patients. Pearson correlation and Cox regression analyses identified disulfidptosis‐related lncRNAs associated with SKCM prognosis. ROC curves and a nomogram validated the model. TME, immune infiltration, GSEA analysis, immune checkpoint gene expression profiling, and drug sensitivity were assessed in high and low‐risk groups. Consistent clustering categorized SKCM patients for personalized clinical treatment guidance.

Results

A total of twelve disulfidptosis‐related lncRNAs were identified for the development of prognosis prediction models. The area under the curve (AUC) values of the ROC curve and the nomogram provided reliable discrimination to evaluate the prognostic potential for SKCM patients. The TME played a crucial role in tumorigenesis, progression and prognosis, and the risk scores were closely related to immune cell infiltration. Meanwhile, the combination of chemotherapy, targeted therapy, and immunotherapy was recommended for low‐risk patients based on drug sensitivity and immune efficacy analyses.

Conclusion

We identified a risk model of twelve disulfidptosis‐related lncRNAs that could be used to predict the prognosis of SKCM patients and help guide immunotherapy and chemotherapy for personalized treatment plans.
Discovering spatial co‐location patterns of urban facilities and their asymmetric characteristics
Authors: Jin Sijia , Yi Disheng , Yuan Junlei , Zhao Yuxin , Qin Jiahiu , Zhou Huijun , Zhang Jing
Published: June 2024

Spatial co‐location pattern (CP) mining can discover sets of geographical features frequently appearing in adjacent locations, which is valuable for comprehending the co‐occurrence relationship between features. However, due to the quantitative differences and heterogeneous distribution of features, the probabilities that features appear in each other's neighborhood are unequal, resulting in an asymmetric spatial pattern. Current studies have paid little attention to the asymmetric characteristics of CPs. Therefore, this study explores the CPs and their asymmetric relationships. Firstly, we adopt the weighted participation index to evaluate the frequency of global candidate CPs. Secondly, we employ an asymmetry index we developed and the local co‐location quotient to quantify the asymmetry intensity of CPs. The results indicate that the frequent CPs mainly comprise facilities related to the residents' daily lives. Investigating the asymmetric relationships and spatial associations among features in the CPs is significant for identifying resource shortages and rationally planning urban resources.
Physiological responses of pepper (Capsicum annum) to combined ozone and pathogen stress
Authors: Modelski Collin , Potnis Neha , Sanz‐Saez Alvaro , Leisner Courtney P.
Published: June 2024

Tropospheric ozone [O3] is a secondary air pollutant formed from the photochemical oxidation of volatile organic compounds in the presence of nitrogen oxides, and it is one of the most damaging air pollutants to crops. O3 entry into the plant generates reactive oxygen species leading to cellular damage and oxidative stress, leading to decreased primary production and yield. Increased O3 exposure has also been shown to have secondary impacts on plants by altering the incidence and response to plant pathogens. We used the Capsicum annum (pepper)‐Xanthomonas perforans pathosystem to investigate the impact of elevated O3 (eO3) on plants with and without exposure to Xanthomonas, using a disease‐susceptible and disease‐resistant pepper cultivar. Gas exchange measurements revealed decreases in diurnal photosynthetic rate (A′) and stomatal conductance (), and maximum rate of electron transport (Jmax) in the disease‐resistant cultivar, but no decrease in the disease‐susceptible cultivar in eO3, regardless of Xanthomonas presence. Maximum rates of carboxylation (Vc,max), midday A and gs rates at the middle canopy, and decreases in aboveground biomass were negatively affected by eO3 in both cultivars. We also observed a decrease in stomatal sluggishness as measured through the Ball–Berry–Woodrow model in all treatments in the disease‐resistant cultivar. We hypothesize that the mechanism conferring disease resistance to Xanthomonas in pepper also renders the plant less tolerant to eO3 stress through changes in stomatal responsiveness. Findings from this study help expand our understanding of the trade‐off of disease resistance with abiotic stresses imposed by future climate change.
Fungal invasion‐induced accumulation of salicylic acid promotes anthocyanin biosynthesis through MdNPR1‐MdTGA2.2 module in apple fruits
Authors: Li Zhao‐Yang , Ma Ning , Sun Ping , Zhang Fu‐Jun , Li Lianzhen , Li Haojian , Zhang Shuai , Wang Xiao‐Fei , You Chun‐Xiang , Zhang Zhenlu
Published: June 2024

In the field, necrosis area induced by pathogens is usually surrounded by a red circle in apple fruits. However, the underlying molecular mechanism of this phenomenon remains unclear. In this study, we demonstrated that accumulated salicylic acid (SA) induced by fungal infection promoted anthocyanin biosynthesis through MdNPR1‐MdTGA2.2 module in apple (Malus domestica). Inoculating apple fruits with Valsa mali or Botryosphaeria dothidea induced a red circle surrounding the necrosis area, which mimicked the phenotype observed in the field. The red circle accumulated a high level of anthocyanins, which was positively correlated with SA accumulation stimulated by fungal invasion. Further analysis showed that SA promoted anthocyanin biosynthesis in a dose‐dependent manner in both apple calli and fruits. We next demonstrated that MdNPR1, a master regulator of SA signaling, positively regulated anthocyanin biosynthesis in both apple and Arabidopsis. Moreover, MdNPR1 functioned as a co‐activator to interact with and enhance the transactivation activity of MdTGA2.2, which could directly bind to the promoters of anthocyanin biosynthetic and regulatory genes to promote their transcription. Suppressing expression of either MdNPR1 or MdTGA2.2 inhibited coloration of apple fruits, while overexpressing either of them significantly promoted fruit coloration. Finally, we revealed that silencing either MdNPR1 or MdTGA2.2 in apple fruits repressed SA‐induced fruit coloration. Therefore, our data determined that fungal‐induced SA promoted anthocyanin biosynthesis through MdNPR1‐MdTGA2.2 module, resulting in a red circle surrounding the necrosis area in apple fruits.
BacillusB2 promotes root growth and enhances phosphorus absorption in apple rootstocks by affecting MhMYB15
Authors: Huang Yimei , Zhai Longmei , Chai Xiaofen , Liu Yao , Lv Jiahong , Pi Ying , Gao Beibei , Wang Xiaona , Wu Ting , Zhang Xinzhong , Han Zhenhai , Wang Yi
Published: June 2024

Due to the chelation of phosphorus in the soil, it becomes unavailable for plant growth and development. The mechanisms by which phosphorus‐solubilizing bacteria activate immobilized phosphorus to promote the growth and development of woody plants, as well as the intrinsic molecular mechanisms, are not clear. Through the analysis of microbial communities in the rhizosphere 16S V3–V4 and a homologous gene encoding microbial alkaline phosphomonoesterase (phoD) in phosphate‐efficient (PE) and phosphate‐inefficient apple rootstocks, it was found that PE significantly enriched beneficial rhizobacteria. The best phosphorus‐solubilizing bacteria, Bacillus sp. strain 7DB1 (B2), was isolated, purified, and identified from the rhizosphere soil of PE rootstocks. Incubating with Bacillus B2 into the rhizosphere of apple rootstocks significantly increased the soluble phosphorus and flavonoid content in the rhizosphere soil. Simultaneously, this process stimulates the root development of the rootstocks and enhances plant phosphorus uptake. After root transcriptome sequencing, candidate transcription factor MhMYB15, responsive to Bacillus B2, was identified through heatmap and co‐expression network analysis. Yeast one‐hybrid, electrophoretic mobility shift assay, and LUC assay confirmed that MhMYB15 can directly bind to the promoter regions of downstream functional genes, including chalcone synthase MhCHS2 and phosphate transporter MhPHT1;15. Transgenic experiments with MhMYB15 revealed that RNAi‐MhMYB15 silenced lines failed to induce an increase in flavonoid content and phosphorus levels in the roots under the treatment of Bacillus B2, and plant growth was slower than the control. In conclusion, MhMYB15 actively responds to Bacillus B2, regulating the accumulation of flavonoids and the uptake of phosphorus, thereby influencing plant growth and development.
Serine hydroxymethyltransferase6 is involved in growth and resistance against pathogens via ethylene and lignin production in Arabidopsis
Authors: Singh Pooja , Kumari Aprajita , Khaladhar Vemula Chandra , Singh Namrata , Pathak Pradeep Kumar , Kumar Vinod , Kumar Ritika Jantu , Jain Priyanka , Thakur Jitendra Kumar , Fernie Alisdair R. , Bauwe Hermann , Raghavendra A.S. , Gupta Kapuganti Jagadis
Published: June 2024

Photorespiratory serine hydroxymethyltransferases (SHMTs) are important enzymes of cellular one‐carbon metabolism. In this study, we investigated the potential role of SHMT6 in Arabidopsis thaliana. We found that SHMT6 is localized in the nucleus and expressed in different tissues during development. Interestingly SHMT6 is inducible in response to avirulent, virulent Pseudomonas syringae and to Fusarium oxysporum infection. Overexpression of SHMT6 leads to larger flowers, siliques, seeds, roots, and consequently an enhanced overall biomass. This enhanced growth was accompanied by increased stomatal conductance and photosynthetic capacity as well as ATP, protein, and chlorophyll levels. By contrast, a shmt6 knockout mutant displayed reduced growth. When challenged with Pseudomonas syringae pv tomato (Pst) DC3000 expressing AvrRpm1, SHMT6 overexpression lines displayed a clear hypersensitive response which was characterized by enhanced electrolyte leakage and reduced bacterial growth. In response to virulent Pst DC3000, the shmt6 mutant developed severe disease symptoms and becomes very susceptible, whereas SHMT6 overexpression lines showed enhanced resistance with increased expression of defense pathway associated genes. In response to Fusarium oxysporum, overexpression lines showed a reduction in symptoms. Moreover, SHMT6 overexpression lead to enhanced production of ethylene and lignin, which are important components of the defense response. Collectively, our data revealed that SHMT6 plays an important role in development and defense against pathogens.
Blood transfusion dynamics in Colombia: Unveiling patterns, reactions and survival rates in multitransfused patients
Authors: Bermúdez‐Forero María‐Isabel , García‐Otálora Michel‐Andrés
Published: June 2024

Background and Objectives

There is no consensus on a universally accepted threshold to categorize a patient as multitransfused. In 2019, Colombia established the definition of a multitransfused patient as someone who has received six or more blood components, irrespective of the time frame. This study aims to delineate the characteristics, adverse transfusion reactions (ATRs, definitions according to the International Society of Blood Transfusion [ISBT]) and survival rates in this population.

Materials and Methods

We performed an analysis from the data of all institutions engaged in blood component transfusions at the national level who notified events to the National Information System of Haemovigilance (SIHEVI‐INS), from January 2018 to December 2022. The selection criteria focused on individuals who not only exhibited ATRs but also received six or more blood components.

Results

Among the 1,784,428 patients who received 6,637,271 blood components, an average of 3.7 components per patient was noted. Concurrently, 8378 ATRs were reported (12.6 ATRs/10,000 transfused components). Within this cohort, 691 patients met the criteria for multitransfusion. Predominantly women (51.8%), these individuals received between 6 and 14 blood components. Out of the 691 multitransfused individuals who experienced ATR, 541 had an allergic reaction. Conversely, out of the 6479 non‐multitransfused individuals who experienced ATR, 3835 had an allergic reaction (odds ratio: 2.49, 95% confidence interval: 2.06–3.0). Notably, 271 multitransfused individuals (39.2%) were documented as deceased, with 76% succumbing within 12 months of encountering their most recent ATR.

Conclusion

Multitransfused individuals in Colombia, being a high‐risk group, exhibit a heightened susceptibility to allergic reactions, surpassing the frequency observed in other transfusion populations. This underscores the necessity for tailored medical care specific to this group.
Cardiopulmonary haemodynamics in Tibetans and Han Chinese during rest and exercise
Authors: Möller Fabian N. , Fan Jui‐Lin , Futral Joel E. , Hodgman Charles F. , Kayser Bengt , Lovering Andrew T.
Published: June 2024

During sea‐level exercise, blood flow through intrapulmonary arteriovenous anastomoses (IPAVA) in humans without a patent foramen ovale (PFO) is negatively correlated with pulmonary pressure. Yet, it is unknown whether the superior exercise capacity of Tibetans well adapted to living at high altitude is the result of lower pulmonary pressure during exercise in hypoxia, and whether their cardiopulmonary characteristics are significantly different from lowland natives of comparable ancestry (e.g. Han Chinese). We found a 47% PFO prevalence in male Tibetans (n = 19) and Han Chinese (n = 19) participants. In participants without a PFO (n = 10 each group), we measured heart structure and function at rest and peak oxygen uptake (), peak power output (), pulmonary artery systolic pressure (PASP), blood flow through IPAVA and cardiac output () at rest and during recumbent cycle ergometer exercise at 760 Torr (SL) and at 410 Torr (ALT) barometric pressure in a pressure chamber. Tibetans achieved a higher than Han, and a higher at ALT without differences in heart rate, stroke volume or . Blood flow through IPAVA was generally similar between groups. Increases in PASP and total pulmonary resistance at ALT were comparable between the groups. There were no differences in the slopes of PASP plotted as a function of during exercise. In those without PFO, our data indicate that the superior aerobic exercise capacity of Tibetans over Han Chinese is independent of cardiopulmonary features and more probably linked to differences in local muscular oxygen extraction.

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Key points

Patent foramen ovale (PFO) prevalence was 47% in Tibetans and Han Chinese living at 2 275 m. Subjects with PFO were excluded from exercise studies.

Compared to Han Chinese, Tibetans had a higher peak workload with acute compression to sea level barometric pressure (SL) and acute decompression to 5000 m altitude (ALT).

Comprehensive cardiac structure and function at rest were not significantly different between Han Chinese and Tibetans.

Tibetans and Han had similar blood flow through intrapulmonary arteriovenous anastomoses (IPAVA) during exercise at SL.

Peak pulmonary artery systolic pressure (PASP) and total pulmonary resistance were different between SL and ALT, with significantly increased PASP for Han compared to Tibetans at ALT. No differences were observed between groups at acute SL and ALT.
Development of a real-time PCR test system for the identification of Francisella tularensis
Authors: Dauletov A. , Ostrovskii A. , Tursunbay N. , Kairzhanova A. , Mukanov K. , Amirgazin A. , Izbanova U. , Shevtsov A.
Published: June 2024

Tularaemia is caused by the Gram-negative bacterium Francisella tularensis, which has three subspecies: holarctica, mediacia and tularensis. Due to the high virulence of the pathogen, the wide range of susceptible animals and the presence of numerous vectors and natural reservoirs, the development of sensitive diagnostic methods for the epidemiological surveillance of tularemia is crucial.  New opportunities for understanding the epidemiological situation of tularemia are opening up with the use of new molecular analysis technologies for typing F. tularensis. The development and use of molecular methods for the diagnosis of tularemia is important in this context. The present study developed a protocol for detection of F. tularensis by real-time polymerase chain reaction (qPCR). The selection of primers and a fluorescent probe was based on the aligned sequences of ISFtu1 (multi-copy insertion element). qPCR conditions were optimised, including the determination of the optimal annealing and extension temperatures for the primers. Sensitivity was tested using successive 4-fold dilutions of DNA derived from F. tularensis subsp. mediasiatica, indicating a minimum sensitivity threshold of 15 genomic equivalents per reaction. Two subspecies of F. tularensis (subsp. mediasiatica and subsp. holarctica), 27 non-target bacterial species and 3 eukaryotic organisms were used to assess specificity.
An Integrated Approach to Designing Robust Gas-Bearing Supported Turbocompressors Through Surrogate Modeling and Constrained All-At-Once Multi-Objective Optimization
Authors: Massoudi Soheyl , Picard Cyril , Schiffmann Jürg
Published: June 2024

This research introduces an innovative framework to engineering design to tackle the challenges of robustness against manufacturing deviations and holistic optimization simultaneously in a multi-disciplinary, multi-subsystems context. The methodology is based on an application of ensemble artificial neural networks, which significantly accelerates computational processes. Coupled with the non-dominated sorting genetic algorithm III, this approach facilitates efficient multi-objective optimization, yielding a comprehensive Pareto front and high-quality design solutions. Here, the framework is applied to the design of gas-bearing-supported turbocompressors. These systems are challenging due to their sensitivity to manufacturing variations, particularly in the gas-bearing geometry, which can lead to rotordynamic instability. Additionally, the interdependencies between the subsystems, such as axial and journal bearings, rotor, compressor impellers, and magnets, necessitate a multidisciplinary approach that spans aerodynamics, structural dynamics, rotordynamics, mechanics, loss analyses, and more. A clear tradeoff between system efficiency, mass-flow range, and robustness has been identified for the compressor design. Higher nominal compressor mass-flows, i.e., increased nominal power, is suggested to decrease the hypervolume of feasible manufacturing deviations. Hence, there is a sweet power spot for gas-bearing supported turbomachinery. Further, the framework’s computational efficiency is on par with that of a university cluster, while only employing a desktop computer equipped with a consumer-grade graphics card. This work demonstrates a significant advancement in the design of complex engineering systems and sets a new standard for speed and efficiency in computational engineering design.
Model Parameter Calibration for Vibration Fatigue Analysis by Means of Bayesian Updating and Artificial Neural Network Based Surrogate Models
Authors: Maier S. , Saenz-Betancourt C. C. , Bachmann F. , Feldmann R. , Götz B. , Duddeck F. , Melz T.
Published: June 2024

In this paper, a methodology for model parameter calibration for vibration fatigue analysis is proposed. It combines Bayesian updating of uncertain model parameters and artificial neural networks (ANNs). The calibrated parameters are used to increase the accuracy of fatigue lifetime calculations for components submitted to vibrational loads. The Bayesian updating uses eigenfrequencies, mode shapes, total mass, and the frequency response functions (FRFs). These quantities are predicted by ANN-based surrogate models to accelerate the Bayesian updating process. A novel strategy for the prediction of the magnitude and phase of FRFs with ANNs is proposed. The frequency is used as an additional input variable, and a schematic selection of significant points of the FRF curves is presented. A high prediction accuracy of the surrogate models could be achieved. The procedure includes the analysis of the relevant frequency range and a sensitivity analysis based on the Morris method to identify appropriate modes and the most-influential parameters. The proposed framework is applied to a current vehicle component subjected to vibrational loads. An experimental modal analysis is used for the calibration and consideration of real parameter uncertainty. First, the accuracy of the surrogate models and Bayesian updating is verified by a nominal reference simulation and then validated with experimental data. The measurable control parameter thickness and component mass are used to examine the calibration accuracy. Finally, a decrease in the dispersion of the vibration fatigue distribution is obtained with the calibrated parameters.
High power impulse magnetron sputtering of a zirconium target
Authors: Suresh Babu Swetha , Fischer Joel , Barynova Kateryna , Rudolph Martin , Lundin Daniel , Gudmundsson Jon Tomas
Published: June 2024

High power impulse magnetron sputtering (HiPIMS) discharges with a zirconium target are studied experimentally and by applying the ionization region model (IRM). The measured ionized flux fraction lies in the range between 25% and 59% and increases with increased peak discharge current density ranging from 0.5 to 2 A/cm2 at a working gas pressure of 1 Pa. At the same time, the sputter rate-normalized deposition rate determined by the IRM decreases in accordance with the HiPIMS compromise. For a given discharge current and voltage waveform, using the measured ionized flux fraction to lock the model, the IRM provides the temporal variation of the various species and the average electron energy within the ionization region, as well as internal discharge parameters such as the ionization probability and the back-attraction probability of the sputtered species. The ionization probability is found to be in the range 73%–91%, and the back-attraction probability is in the range 67%–77%. Significant working gas rarefaction is observed in these discharges. The degree of working gas rarefaction is in the range 45%–85%, higher for low pressure and higher peak discharge current density. We find electron impact ionization to be the main contributor to working gas rarefaction, with over 80% contribution, while kick-out by zirconium atoms and argon atoms from the target has a smaller contribution. The dominating contribution of electron impact ionization to working gas rarefaction is very similar to other low sputter yield materials.
Caffeine Gum Improves Reaction Time but Reduces Composure Versus Placebo During the Extra-Time Period of Simulated Soccer Match-Play in Male Semiprofessional Players
Authors: Field Adam , Corr Liam , Birdsey Laurence , Langley Christina , Marshall Ben , Wood Greg , Hearris Mark , Martinho Diogo , Carbry Christa , Naughton Robert , Fleming James , Mohr Magni , Krustrup Peter , Russell Mark , David Harper Liam
Published: June 2024

This study aimed to determine whether caffeine gum influenced perceptual-cognitive and physical performance during the extra-time period of simulated soccer match-play. Semiprofessional male soccer players (n = 12, age: 22 ± 3 years, stature: 1.78 ± 0.06 m, mass: 75 ± 9 kg) performed 120-min soccer-specific exercise on two occasions. In a triple-blind, randomized, crossover design, players chewed caffeinated (200 mg; caffeine) or control (0 mg; placebo) gum for 5 min following 90 min of soccer-specific exercise. Perceptual-cognitive skills (i.e., passing accuracy, reaction time, composure, and adaptability) were assessed using a soccer-specific virtual reality simulator, collected pre- and posttrial. Neuromuscular performance (reactive-strength index, vertical jump height, absolute and relative peak power output, and negative vertical displacement) and sprint performance (15 and 30 m) were measured at pretrial, half-time, 90 min, and posttrial. Caffeine gum attenuated declines in reaction time (pre: 90.8 ± 0.8 AU to post: 90.7 ± 0.8 AU) by a further 4.2% than placebo (pre: 92.1 ± 0.8 AU to post: 88.2 ± 0.8 AU; p < .01). Caffeine gum reduced composure by 4.7% (pre: 69.1 ± 0.8 AU to post: 65.9 ± 0.8 AU) versus placebo (pre: 68.8 ± 0.8 AU to post: 68.3 ± 0.8 AU; p < .01). Caffeine gum did not influence any other variables (p > .05). Where caffeine gum is consumed by players prior to extra-time, reaction time increases but composure may be compromised, and neuromuscular and sprint performance remain unchanged. Future work should assess caffeine gum mixes with substances like L-theanine that promote a relaxed state under stressful conditions.
Football Movement Profile–Based Creatine-Kinase Prediction Performs Similarly to Global Positioning System–Derived Machine Learning Models in National-Team Soccer Players
Authors: Schuth Gabor , Szigeti György , Dobreff Gergely , Pašić Alija , Gabbett Tim , Szilas Adam , Pavlik Gabor
Published: June 2024

Purpose: The relationship between external load and creatine-kinase (CK) response at the team/position or individual level using Global Positioning Systems (GPS) has been studied. This study aimed to compare GPS-derived and Football Movement Profile (FMP) –derived CK-prediction models for national-team soccer players. The second aim was to compare the performance of general and individualized CK prediction models. Methods: Four hundred forty-four national-team soccer players (under 15 [U15] to senior) were monitored during training sessions and matches using GPS. CK was measured every morning from whole blood. The players had 19.3 (18.1) individual GPS-CK pairs, resulting in a total of 8570 data points. Machine learning models were built using (1) GPS-derived or (2) FMP-based parameters or (3) the combination of the 2 to predict the following days’ CK value. The performance of general and individual-specific prediction models was compared. The performance of the models was described by R2 and the root-mean-square error (RMSE, in units per liter for CK values). Results: The FMP model (R2 = .60, RMSE = 144.6 U/L) performed similarly to the GPS-based model (R2 = .62, RMSE = 141.2 U/L) and the combination of the 2 (R2 = .62, RMSE = 140.3 U/L). The prediction power of the general model was better on average (R2 = .57 vs R2 = .37) and for 73% of the players than the individualized model. Conclusions: The results suggest that FMP-based CK-prediction models perform similarly to those based on GPS-derived metrics. General machine learning models’ prediction power was higher than those of the individual-specific models. These findings can be used to monitor postmatch recovery strategies and to optimize weekly training periodization.