bluuebunny/crossref_metadata_2025 huggingface dataset:
Zearalenone exposure differentially affects the ovarian proteome in pre-pubertal gilts during thermal neutral and heat stress conditions
Authors: Roach Crystal M , Mayorga Edith J , Baumgard Lance H , Ross Jason W , Keating Aileen F
Published: April 2024

Zearalenone (ZEN), a nonsteroidal estrogenic mycotoxin, causes endocrine disruption and porcine reproductive dysfunction. Heat stress (HS) occurs when exogenous and metabolic heat accumulation exceeds heat dissipation. Independently, HS and ZEN both compromise swine reproduction; thus, the hypothesis investigated was two-pronged: that ZEN exposure would alter the ovarian proteome and that these effects would differ in thermal neutral (TN) and HS pigs. Pre-pubertal gilts (n = 38) were fed ad libitum and assigned to either (TN: 21.0 ± 0.1 °C) or HS (12 h cyclic temperatures of 35.0 ± 0.2 °C and 32.2 ± 0.1 °C). Within the TN group, a subset of pigs were pair-fed (PF) to the amount of feed that the HS gilts consumed to eliminate the confounding effects of dissimilar nutrient intake. All gilts orally received a vehicle control (CT) or ZEN (40 μg/kg/BW) resulting in six treatment groups: thermoneutral (TN) vehicle control (TC; n = 6); TN ZEN (TZ; n = 6); PF vehicle control (PC; n = 6); PF ZEN (PZ; n = 6); HS vehicle control (HC; n = 7); or HS ZEN (HZ; n = 7) for 7 d. When compared to the TC pigs, TZ pigs had 45 increased and 39 decreased proteins (P ≤ 0.05). In the HZ pigs, 47 proteins were increased and 61 were decreased (P ≤ 0.05). Exposure to ZEN during TN conditions altered sec61 translocon complex (40%), rough endoplasmic reticulum membrane (8.2%), and proteasome complex (5.4%), asparagine metabolic process (0.60%), aspartate family amino acid metabolic process (0.14%), and cellular amide metabolic process (0.02%) pathways. During HS, ZEN affected cellular pathways associated with proteasome core complex alpha subunit complex (0.23%), fibrillar collagen trimer (0.14%), proteasome complex (0.05%), and spliceosomal complex (0.03%). Thus, these data identify ovarian pathways altered by ZEN exposure and suggest that the molecular targets of ZEN differ in TN and HS pigs.
‘Now completely Americanized’: Collecting and transatlantic exchange of the Lansdowne Marbles
Authors: Cochrane Nicole
Published: April 2024

This article examines the sale in 1930 by Christie’s of the collection of ancient marbles formerly belonging to William Petty-Fitzmaurice, 1st Marquess of Lansdowne, and the role that the auction played in the transatlantic trade in antiquities between Britain and the United States in the early twentieth century. It examines how provenance and collecting histories, and the prestige engendered by owning antiquities from a notable collection, became an important marker of taste and desirability for American collectors.
Feasibility and effectiveness of preconception check-ups at workplaces in Japan
Authors: Fujishima Akiko , Maeda Eri , Sato Koki , Saito Hidekazu , Ozeki Chihiro , Terada Yukihiro
Published: April 2024

Objectives

Despite the recent increase in infertility and perinatal complications, preconception care is not commonly available in Japan. Working women are considered to have the greatest need for preconception care, as increasingly they marry and have children later in life. This study aimed to assess the feasibility and effectiveness of preconception check-ups in the workplace.

Methods

We provided 51 female employees aged 18-39 years with free preconception check-ups, including additional blood tests and an online medical questionnaire, during mandatory health check-ups at their workplace. A doctor provided online counseling based on the check-up results. We assessed fertility knowledge using the Cardiff Fertility Knowledge Scale (CFKS-J) and childbearing desire pre- and postintervention.

Results

Preconception check-ups revealed various potential risk factors for future pregnancies, including underweight (12%), obesity (20%), Chlamydia trachomatis IgG antibody positivity (22%), low rubella IgG antibody levels (47%), iron deficiency (12%), and 25-hydroxyvitamin D levels <30 ng/mL (98%). Postintervention, the participants reported high satisfaction with the check-ups and significantly advanced their reproductive plans (P = .008). Furthermore, 95% of the participants indicated an intention to seek medical attention or make lifestyle changes. The postintervention CFKS-J score (mean [SD]) was higher than the preintervention score (71.7 [19.3] vs 63.0 [22.0]; P = .006).

Conclusions

We developed a preconception check-up package that can be integrated into workplace health examinations, complemented by tailored counseling. This novel check-up package is a feasible and effective approach for improving preconception health and fertility awareness.
Quanzhen Yiqi decoction attenuates inflammation in mice with smoking-induced COPD by activating the Nrf2/HO-1 pathway and inhibiting the NLRP3 inflammasome
Authors: Zeng Jiamin , Li Zhenqiu , Li Wanyan , Liang Zhu , Luo Yuewen , Li Caiping , Liao Sida , Wang Kexin , Hu Yuanlong , Li Yuanyuan , Liang Qiuling , Lu Wenju , Li Lu , Wu Zhijuan , Zhang Dapeng , Zhang Zhimin
Published: April 2024

Objective

Quanzhen Yiqi decoction (QZYQ) is a traditional Chinese medicine for treating chronic obstructive pulmonary disease.

Methods

Mice were exposed to cigarette smoke (CS) 6 days/week (40 cigarettes/day) for 24 weeks and then intragastrically administered QZYQ (4.72, 9.45, or 18.89 g/kg) or dexamethasone (DEX, 0.6 mg/kg) for 6 weeks. We examined the lung function and collected bronchoalveolar lavage fluid for inflammatory cell and cytokine quantification. The pathological lung changes, ROS and oxidative biomarkers were measured. We used immunohistochemistry and western blotting to evaluate the levels of Nrf2/HO-1, NLRP3/ASC/Caspase1/IL-1β/IL-18.

Results

The CS group showed significant increases in the forced vital capacity, lung resistance, and chord compliance and a lower FEV50/FVC compared with the control, and QZYQ improved these changes. In addition, QZYQ effectively reduced emphysema, immune cell infiltration, and airway remodeling. QZYQ stimulated HO-1 expression and reduced oxidative stress through the Nrf2 pathway. QZYQ inhibited the production of NLRP3/ASC/Caspase-1 to inhibit IL-1β and IL-18.

Conclusion

Our study suggested that QZYQ can improve the function and histology of the lungs and reduce inflammatory cell recruitment. QZYQ inhibits ROS production and NLRP3 inflammasome activation by upregulating Nrf2 to reduce lung injury. The anti-inflammatory effects of QZYQ are similar to those of DEX.
Redox regulation of meristem quiescence: outside/in
Authors: Considine Michael J , Foyer Christine H
Published: April 2024

Quiescence is an essential property of meristematic cells, which restrains the cell cycle while retaining the capacity to divide. This crucial process not only facilitates life-long tissue homeostasis and regenerative capacity but also provides protection against adverse environmental conditions, enabling cells to conserve the proliferative potency while minimizing DNA damage. As a survival attribute, quiescence is inherently regulated by the products of aerobic life, in particular reactive oxygen species (ROS) and the redox (reduction/oxidation) mechanisms that plant have evolved to channel these into pervasive signals. Adaptive responses allow quiescent cells to compensate for reduced oxygen tension (hypoxia) in a reversible manner, while the regulated production of the superoxide anion (O2·−) facilitates cell division and the maintenance of stem cells. Here we discuss the role of ROS and redox reactions in the control of the quiescent state in plant meristems, and how this process is integrated with cellular energy and hormone biochemistry. We consider the pathways that sense and transmit redox signals with a focus on the central significance of redox regulation in the mitochondria and nucleus, which is a major regulator of quiescence in meristems. We discuss recent studies that suggest that ROS are a critical component of the feedback loops that control stem cell identity and fate, and suggest that the ROS/hypoxia interface is an important ‘outside/in’ positional cue for plant cells, particularly in meristems.
Mitochondrial transplantation for the treatment of cardiac and noncardiac diseases: mechanisms, prospective, and challenges
Authors: Wang Xinyi , Liu Zhiyuan , Zhang Ling , Hu Guangyu , Tao Ling , Zhang Fuyang
Published: April 2024

Mitochondrial transplantation (MT) is a promising therapeutic strategy that involves introducing healthy mitochondria into damaged tissues to restore cellular function. This approach has shown promise in treating cardiac diseases, such as ischemia-reperfusion injury, myocardial infarction, and heart failure, where mitochondrial dysfunction plays a crucial role. Transplanting healthy mitochondria into affected cardiac tissue has resulted in improved cardiac function, reduced infract size, and enhanced cell survival in preclinical studies. Beyond cardiac applications, MT is also being explored for its potential to address various noncardiac diseases, including stroke, infertility, and genetic mitochondrial disorders. Ongoing research focused on refining techniques for mitochondrial isolation, preservation, and targeted delivery is bolstering the prospects of MT as a clinical therapy. As the scientific community gains a deeper understanding of mitochondrial dynamics and pathology, the development of MT as a clinical therapy holds significant promise. This review provides an overview of recent research on MT and discusses the methodologies involved, including sources, isolation, delivery, internalization, and distribution of mitochondria. Additionally, it explores the effects of MT and potential mechanisms in cardiac diseases, as well as non-cardiac diseases. Future prospects for MT are also discussed.
‘Formalistic Freaks in Music’: ‘Ilya Golovin’, Shostakovich, and Zhdanovshchina for the Masses
Authors: Elphick Daniel
Published: April 2024

Amidst all the art, literature, music, and theatre of the ‘Zhdanovshchina’ (the post-war Soviet intervention in arts policy), one play stands out: Sergei Mikhalkov’s Ilya Golovin. In this play, a leading Soviet composer is chastised for his formalism but sees the error of his ways and again writes music of sufficiently socialist character. Ilya Golovin is noteworthy not just because it presents a crude parody of Shostakovich and his music, but because it reveals the wider context of the ideological campaign against artists and shows us something of how different artists responded. In this article, I argue that Ilya Golovin is not simply a bizarre example of multiple artists toeing the ideological line: instead it reveals the uglier side of late Stalinism in the form of vehement anti-Semitism, as well as demonstrating the xenophobic Cold War patriotism that served as the ultimate motivation behind the Zhdanovshchina itself.
Cosmological prediction of the CSST Ultra Deep Field Type Ia supernova photometric survey
Authors: Wang Minglin , Gong Yan , Deng Furen , Miao Haitao , Chen Xuelei , Zhan Hu
Published: April 2024

Type Ia supernova (SN Ia) as a standard candle is an ideal tool to measure cosmic distance and expansion history of the Universe. Here, we investigate the SN Ia photometric measurement in the China Space Station Telescope Ultra Deep Field (CSST-UDF) survey, and study the constraint power on the cosmological parameters, such as the equation of state of dark energy. The CSST-UDF survey is expected to cover a 9 deg2 sky area in 2 yr with 250 s × 60 exposures for each band. The magnitude limit can reach i ≃ 26 AB mag for 5σ point source detection with a single exposure. We generate light-curve mock data for SNe Ia and different types of core-collapse SNe (CCSNe). sncosmo is chosen as the framework by utilizing the salt3 model to simulate SN Ia data. After selecting high-quality data and fitting the light curves, we derive the light-curve parameters and identify CCSNe as contamination, resulting in ∼2200 SNe with an $\sim\!\! 7{{\ \rm per\, cent}}$ CCSN contamination rate. We adopt a calibration method similar to Chauvenet’s criterion, and apply it to the distance modulus data to further reduce the contamination. We find that this method is effective and can suppress the contamination fraction to $\sim\!\! 3.5{{\ \rm per\, cent}}$ with 2012 SNe Ia and 73 CCSNe. In the cosmological fitting stage, we did not distinguish between SNe Ia and CCSNe. We find that the constraint accuracies on ΩM, ΩΛ, and w are about two times better than the current SN surveys, and they could be further improved by a factor of ∼1.4 if including the baryon acoustic oscillation data from the CSST spectroscopic wide-field galaxy survey.
Constraints on cosmological models from quasars calibrated with type Ia supernova by a Gaussian process
Authors: Zhang Haixiang , Liu Yang , Yu Hongwei , Nong Xiaodong , Liang Nan , Wu Puxun
Published: April 2024

In this paper, we use quasars calibrated from type Ia supernova (SN Ia) to constrain cosmological models. We consider three different X-ray luminosity (LX)–ultraviolet luminosity (LUV) relations of quasars, i.e. the standard LX–LUV relation and two redshift–evolutionary relations (Type I and Type II), respectively, constructed from copula and considering a redshift correction to the luminosity of quasars. Only in the case of the Type I relation, quasars can always provide effective constraints on the ΛCDM (cosmological constant Λ plus cold dark matter) model. Furthermore, we show that, when the observational Hubble data (OHD) are added, the constraints on the absolute magnitude M of SN Ia and the Hubble constant H0 can be obtained. In the ΛCDM model, the OHD measurements plus quasars with the Type I relation yields M = $-19.321^{+0.085}_{-0.076}$, which is in good agreement with the measurement from SH0ES (M = −19.253 ± 0.027), and H0 = $70.80\pm 3.6~\mathrm{km~s^{-1}\, Mpc^{-1}}$, falling between the measurements from SH0ES and the Planck cosmic microwave background radiation data.
Mapper of the IGM spin temperature: instrument overview
Authors: Monsalve R A , Altamirano C , Bidula V , Bustos R , Bye C H , Chiang H C , Díaz M , Fernández B , Guo X , Hendricksen I , Hornecker E , Lucero F , Mani H , McGee F , Mena F P , Pessôa M , Prabhakar G , Restrepo O , Sievers J L , Thyagarajan N
Published: April 2024

The observation of the global 21 cm signal produced by neutral hydrogen gas in the intergalactic medium (IGM) during the Dark Ages, Cosmic Dawn, and Epoch of Reionization requires measurements with extremely well-calibrated wideband radiometers. We describe the design and characterization of the Mapper of the IGM Spin Temperature (MIST), which is a new ground-based, single-antenna, global 21 cm experiment. The design of MIST was guided by the objectives of avoiding systematics from an antenna ground plane and cables around the antenna, as well as maximizing the instrument’s on-sky efficiency and portability for operations at remote sites. We have built two MIST instruments, which observe in the range 25–105 MHz. For the 21 cm signal, this frequency range approximately corresponds to redshifts 55.5 > z > 12.5, encompassing the Dark Ages and Cosmic Dawn. The MIST antenna is a horizontal blade dipole of 2.42 m in length, 60 cm in width, and 52 cm in height above the ground. This antenna operates without a metal ground plane. The instruments run on 12 V batteries and have a maximum power consumption of 17 W. The batteries and electronics are contained in a single receiver box located under the antenna. We present the characterization of the instruments using electromagnetic simulations and lab measurements. We also show sample sky measurements from recent observations at remote sites in California, Nevada, and the Canadian High Arctic. These measurements indicate that the instruments perform as expected. Detailed analyses of the sky measurements are left for future work.
Implications on star formation rate indicators from H ii regions and diffuse ionized gas in the M101 Group
Authors: Watkins A E , Mihos J C , Harding P , Garner R
Published: April 2024

We examine the connection between diffuse ionized gas (DIG), H ii regions, and field O and B stars in the nearby spiral M101 and its dwarf companion NGC 5474 using ultra-deep H α narrow-band imaging and archival GALEX UV imaging. We find a strong correlation between DIG H α surface brightness and the incident ionizing flux leaked from the nearby H ii regions, which we reproduce well using simple cloudy simulations. While we also find a strong correlation between H α and co-spatial far-ultraviolet (FUV) surface brightness in DIG, the extinction-corrected integrated UV colours in these regions imply stellar populations too old to produce the necessary ionizing photon flux. Combined, this suggests that H ii region leakage, not field OB stars, is the primary source of DIG in the M101 Group. Corroborating this interpretation, we find systematic disagreement between the H α- and FUV-derived star formation rates (SFRs) in the DIG, with SFRH α <SFRFUV everywhere. Within H ii regions, we find a constant SFR ratio of 0.44 to a limit of ∼10−5 M⊙ yr−1. This result is in tension with other studies of star formation in spiral galaxies, which typically show a declining SFRH α/SFRFUV ratio at low SFR. We reproduce such trends only when considering spatially averaged photometry that mixes H ii regions, DIG, and regions lacking H α entirely, suggesting that the declining trends found in other galaxies may result purely from the relative fraction of diffuse flux, leaky compact H ii regions, and non-ionizing FUV-emitting stellar populations in different regions within the galaxy.
Senescence of human pancreatic beta cells enhances functional maturation through chromatin reorganization and promotes interferon responsiveness
Authors: Patra Milan , Klochendler Agnes , Condiotti Reba , Kaffe Binyamin , Elgavish Sharona , Drawshy Zeina , Avrahami Dana , Narita Masashi , Hofree Matan , Drier Yotam , Meshorer Eran , Dor Yuval , Ben-Porath Ittai
Published: April 2024

Senescent cells can influence the function of tissues in which they reside, and their propensity for disease. A portion of adult human pancreatic beta cells express the senescence marker p16, yet it is unclear whether they are in a senescent state, and how this affects insulin secretion. We analyzed single-cell transcriptome datasets of adult human beta cells, and found that p16-positive cells express senescence gene signatures, as well as elevated levels of beta-cell maturation genes, consistent with enhanced functionality. Senescent human beta-like cells in culture undergo chromatin reorganization that leads to activation of enhancers regulating functional maturation genes and acquisition of glucose-stimulated insulin secretion capacity. Strikingly, Interferon-stimulated genes are elevated in senescent human beta cells, but genes encoding senescence-associated secretory phenotype (SASP) cytokines are not. Senescent beta cells in culture and in human tissue show elevated levels of cytoplasmic DNA, contributing to their increased interferon responsiveness. Human beta-cell senescence thus involves chromatin-driven upregulation of a functional-maturation program, and increased responsiveness of interferon-stimulated genes, changes that could increase both insulin secretion and immune reactivity.
EDA2R reflects the acute brain response to cranial irradiation in liquid biopsies
Authors: Lastra Romero Alejandro , Seitz Thea , Zisiadis Georgios Alkis , Jeffery Holli , Osman Ahmed M
Published: April 2024

Background

Cranial radiotherapy is standard of care for high-grade brain tumors and metastases; however, it induces debilitating neurocognitive impairments in cancer survivors, especially children. As the numbers of pediatric brain cancer survivors continue improving, the numbers of individuals developing life-long neurocognitive sequalae are consequently expected to rise. Yet, there are no established biomarkers estimating the degree of the irradiation-induced brain injury at completion of radiotherapy to predict the severity of the expected neurocognitive complications. We aimed to identify sensitive biomarkers associated with brain response to irradiation that can be measured in easily accessible clinical materials, such as liquid biopsies.

Methods

Juvenile mice were subjected to cranial irradiation with 0.5, 1, 2, 4, and 8 Gy. Cerebrospinal fluid (CSF), plasma, and brains were collected at acute, subacute, and subchronic phases after irradiation, and processed for proteomic screens, and molecular and histological analyses.

Results

We found that the levels of ectodysplasin A2 receptor (EDA2R), member of tumor necrosis factor receptor superfamily, increased significantly in the CSF after cranial irradiation, even at lower irradiation doses. The levels of EDA2R were increased globally in the brain acutely after irradiation and decreased over time. EDA2R was predominantly expressed by neurons, and the temporal dynamics of EDA2R in the brain was reflected in the plasma samples.

Conclusions

We propose EDA2R as a promising potential biomarker reflecting irradiation-induced brain injury in liquid biopsies. The levels of EDA2R upon completion of radiotherapy may aid in predicting the severity of IR-induced neurocognitive sequalae at a very early stage after treatment.
An all-metallic nanovesicle for hydrogen oxidation
Authors: Zhang Juntao , Jin Lujie , Sun Hao , Liu Xiaozhi , Ji Yujin , Li Youyong , Liu Wei , Su Dong , Liu Xuerui , Zhuang Zhongbin , Hu Zhiwei , Shao Qi , Huang Xiaoqing
Published: April 2024

Vesicle, a microscopic unit that encloses a volume with an ultrathin wall, is ubiquitous in biomaterials. However, it remains a huge challenge to create its inorganic metal-based artificial counterparts. Here, inspired by the formation of biological vesicles, we proposed a novel biomimetic strategy of curling the ultrathin nanosheets into nanovesicles, which was driven by the interfacial strain. Trapped by the interfacial strain between the initially formed substrate Rh layer and subsequently formed RhRu overlayer, the nanosheet begins to deform in order to release a certain amount of strain. Density functional theory (DFT) calculations reveal that the Ru atoms make the curling of nanosheets more favorable in thermodynamics applications. Owing to the unique vesicular structure, the RhRu nanovesicles/C displays excellent hydrogen oxidation reaction (HOR) activity and stability, which has been proven by both experiments and DFT calculations. Specifically, the HOR mass activity of RhRu nanovesicles/C are 7.52 A mg(Rh+Ru)−1 at an overpotential of 50 mV at the rotating disk electrode (RDE) level; this is 24.19 times that of commercial Pt/C (0.31 mA mgPt−1). Moreover, the hydroxide exchange membrane fuel cell (HEMFC) with RhRu nanovesicles/C displays a peak power density of 1.62 W cm−2 in the H2-O2 condition, much better than that of commercial Pt/C (1.18 W cm−2). This work creates a new biomimetic strategy to synthesize inorganic nanomaterials, paving a pathway for designing catalytic reactors.
Feasibility Randomized Controlled Trial of Face-to-Face Counseling and Mobile Phone Messages Compared to Usual Care for Smokeless Tobacco Cessation in Indian Primary Care: Project CERTAIN
Authors: Panda Rajmohan , Omar Rumana , Hunter Rachael , Lahoti Supriya , Prabhu Rajath R , Vickerstaff Victoria , Satapathy Durga Madhab , Das Sangeeta , Nazareth Irwin
Published: April 2024

Introduction

Smokeless tobacco (SLT) use in low- and middle-income countries has adverse health consequences. We hypothesize that it is feasible to test an intervention of mobile phone messages and face-to-face counseling sessions for SLT cessation in India.

Aims and Methods

We conducted an exploratory, individual parallel two group, randomized controlled trial (RCT), with baseline and end-point (3 months from randomization) assessments in urban primary health centers in Odisha, India. A total of 250 current (i.e., users in the last 3 months) SLT users or dual users (ie, smokers and SLT users) were recruited to the trial (125 in each group). Participants were randomized to either routine care, face-to-face counseling, and reminder mobile messages or routine care only. The primary outcomes were to assess the feasibility of running a full RCT including recruitment, compliance, and retention.

Results

A total of seven (77.8%) out of nine primary care centers took part in the trial. Out of the 315 SLT users invited to participate, 250 provided consent and were randomized [79.4% (95% CI: 74.5, 83.7)]. Out of the 250 randomized SLT users, 238 [95% (95% CI: 91.8, 97.5)] were followed up at 3 months (117 in the intervention group and 121 in the control group). Of the participants in the intervention group, 74 (63.8%) reported that they received the mobile messages.

Conclusions

This exploratory trial demonstrated the feasibility of delivering and evaluating an intervention of mobile phone messages and face-to-face counseling for SLT users in Indian primary care in a full randomized trial

Implications

This study found that combining mobile messages with face-to-face counseling for smokeless tobacco users visiting primary health care settings in India is feasible in terms of recruitment of users, compliance with the intervention, and retention of study participants within the trial. The biochemically verified smokeless tobacco abstinence rate was higher in the intervention group compared with the control group. There was poor agreement between self-reported tobacco cessation and the measured salivary cotinine in smokeless tobacco users. The findings support the feasibility and acceptability of the intervention signaling the need for a larger clinical trial to test the effectiveness of the intervention.
Health effects of drinking 100% juice: an umbrella review of systematic reviews with meta-analyses
Authors: Beckett Emma L , Fayet-Moore Flávia , Cassettari Tim , Starck Carlene , Wright Jutta , Blumfield Michelle
Published: April 2024

Context

Low fruit and vegetable intakes are major modifiable determinants of disease. One hundred percent juice may facilitate intake and deliver essential nutrients and bioactive compounds. However, the position of 100% juice in healthy eating guidelines remains controversial due to its lower dietary fiber and higher free-sugar contents compared with whole fruits and vegetables.

Objective

To conduct an umbrella review of systematic literature reviews with meta-analyses (MAs) to summarize the health benefits of drinking 100% fruit and/or vegetable juice.

Data Sources

Four databases (Medline, The Cochrane Library, EMBASE, and CINAHL) were systematically searched for MAs of 100% juice and any health outcomes.

Data Analysis

Screening, quality, risk of bias, and content overlap tools were applied, and extracted data were narratively synthesized. No eligible studies for vegetable juice were found. Fifteen systematic literature reviews (51 primary MAs, 6 dose–response, and 87 subanalyses; 50–1200 mL/day; hours to years of duration) were included. Ten MAs (19.6%) reported health benefits (4 for blood pressure, 2 for vascular function, 3 for inflammation, 1 for stroke mortality), 3 MAs (5.9%) reported adverse risks (1 each for cardiovascular disease mortality, prostate cancer, type 2 diabetes risk), while most (74.5%) reported no effect (blood lipids, body composition, liver function, metabolic health, cancers, and inflammation). Risks were limited to cohort studies and benefits were found in both cohort and intervention studies.

Conclusion

The findings collate evidence showing some potential health benefits associated with 100% juice consumption, with fewer potential risks. The balance of evidence does not support the exclusion of 100% juice from food-based guides to healthy eating, although caution may be warranted in certain groups or individuals, and the body of evidence is not yet conclusive.

Systematic Review Registration

PROSPERO registration no. CRD42022380588.
The risk of complaints against occupational physicians to the medical regulatory body in Ireland
Authors: Gleeson Deirdre
Published: April 2024

Complaints against doctors to regulators are increasing in all jurisdictions. Occupational physicians (OPs) are at increased risk of complaint due to the unique doctor–patient relationship and the dual obligations of the role. OPs may become embroiled in third-party disputes and must be clear that their role is an advisor to the decision-maker, but not the decision-maker. OPs require training in how to reduce, cope with and defend against complaints to regulators.
No Patient Left Behind: A Novel Paradigm to Fulfill Hepatitis C Virus Treatment for Rural Patients
Authors: Gormley Mirinda Ann , Moschella Phillip , Cordero-Romero Susan , Wampler Wesley R , Allison Marie , Kitzmiller Katiey , Estes Luke , Heo Moonseong , Litwin Alain H , Roth Prerana
Published: April 2024

Background

This study evaluates a novel multidisciplinary program providing expanded access to hepatitis C virus (HCV) treatment for rural Appalachian patients in South Carolina. This program identified patients via an opt-out emergency department screening program, and it aimed to achieve HCV cure by using community paramedics (CPs) to link and monitor patients from treatment initiation through 12-week sustained virologic response (SVR).

Methods

Patients aged ≥18 years who were HCV RNA positive were eligible for enrollment if they failed to appear for a scheduled HCV appointment or reported barriers to accessing office-based treatment. CPs provided home visits (initial and 4, 12, and 24 weeks) using a mobile Wi-Fi hotspot to support telemedicine appointments (compliant with the Health Insurance Portability and Accountability Act) and perform focused physical assessments, venipuncture, and coordinated home delivery of medications. Statistics described participant characteristics, prevalence of SVR, and patient satisfaction results at 12 weeks posttreatment.

Results

Thirty-four patients were eligible for SVR laboratory tests by 31 August 2023; the majority were male (61.7%) and White (64.7%) with an average age of 56 years (SD, 11.7). Twenty-eight (82.4%) completed treatment and achieved 12-week SVR. Six (17.6%) were lost to follow-up. Two-thirds strongly agreed that they were satisfied with the overall care that they received, and half strongly agreed that their overall health had improved.

Conclusions

This CP-augmented treatment program demonstrated success curing HCV for rural patients who lacked access to office-based treatment. Other health care systems may consider this novel delivery model to treat hard-to-reach individuals who are HCV positive.
Shared Vision for Improving Outcomes for Serious Fungal Diseases: Report of a Patient, Caregiver, and Clinician Summit
Authors: Purdie Rob , Tushla Lisa A , Ferretti Jonathan , Castro Gonzalo (Kiko) , Watson Ricky , Davis Thomas , Raborg Brianna , Mazi Patrick B , Stroman Angela , Thomas Jones Carolynn , Walsh Thomas J , Chiller Tom M , Pappas Peter G , Meyer John , Spec Andrej
Published: April 2024

Background

Recently, increasing focus on patient input into research and healthcare improvements has fostered expanded patient-centered advocacy efforts. This first pan-fungal disease summit, part of the MYCology Advocacy, Research, & Education effort, brought together patients, caregivers, and mycology experts to better document patient experiences with invasive fungal disease (IFD) and establish priorities for mycology education, advocacy, and research.

Methods

Patients who had suffered from IFD, their caregivers, clinicians, industry representatives, government officials, and patient advocacy professionals were invited. Patients and caregivers shared their stories and struggles with IFD. Breakout sessions separated mycology experts from patients and caregivers for further discussions to identify commonalities and perceived gaps and to formulate recommendations. The 2 groups then reconvened to develop consensus recommendations.

Results

IFD patients and their caregivers shared experiences reflecting the typically lengthy prediagnosis, acute treatment, long-term treatment, and posttreatment recovery stages of IFD. They reported substantial physical, psychological, and financial burdens associated with the IFD experience, particularly related to delayed diagnoses. They reaffirmed a need for coordinated patient-centered education, peer support, and advocacy to document the burden of serious fungal infections. Mycology experts discussed strategies to address gaps in the mycology field, such as insufficient training, inadequate workforce support, and a need to partner more with patient groups.

Conclusions

A summit involving patients with IFD, family caregivers, and mycology experts identified a substantial nonclinical burden of disease associated with IFD. Patients and mycology experts prioritized several goals for education, advocacy, and research to raise awareness of IFD and improve outcomes.
Pharmacist-Driven Rapid Initiation of Antiretroviral Therapy Decreases Time to Viral Suppression in People With HIV
Authors: Brotherton Amy L , Coroniti Ann-Marie , Ayuninjam Diane K , Sanchez Martha C , Benitez Gregorio , Garland Joseph M
Published: April 2024

Background

Rapid initiation of antiretroviral therapy (rapid ART) improves clinical outcomes in people with HIV and is endorsed by clinical guidelines. However, logistical challenges limit widespread implementation. We describe an innovative rapid ART model led by pharmacists and its impact on clinical outcomes, including time to viral suppression (TVS).

Methods

On 1 January 2019, we implemented Pharmacist-Driven Rapid ART (PHARM-D RAPID ART), including rapid ART initiation by pharmacists. Our retrospective cohort study compared TVS, using a Cox proportional hazards model, and clinical outcomes among individuals with a new HIV diagnosis before (1 January 2017 to 31 December 2017) and after (1 January 2019 to 31 December 2019) implementation.

Results

A total of 108 individuals were included. TVS was significantly shorter (P < .001) for the PHARM-D RAPID ART group (n = 51) compared with the preimplementation group (n = 57) (median: 30 days and 66 days, respectively). Those in the PHARM-D RAPID ART group were significantly more likely to achieve VS at any given time during the study period (adjusted hazard ratio: 3.47 [95% confidence interval, 2.25–5.33]). A total of 94.1% (48/51) of patients in the PHARM-D RAPID ART group were retained in care at 1 year. With a median follow-up of 2.4 years in the PHARM-D RAPID ART group, 98% remained suppressed at last recorded viral load.

Conclusions

A pharmacist-driven model for rapid ART delivery decreases TVS with high rates of retention in care and durable VS. This model could improve clinical outcomes and increase program feasibility and sustainability.
Cutaneous Larva Migrans (CLM) may not be easy to diagnose: a case report and narrative review
Authors: Osman Solafa , Tarnari Nectaria , Ahsan Areeba , Ahmed Khabab Abbasher Hussien Mohamed
Published: April 2024

Background: Cutaneous Larva Migrans (CLM) is one of the most common zoonotic dermatoses in subtropical and tropical regions and some European countries. It is caused by different types of hookworm, such as Ancylostoma braziliense, Ancylostoma caninum, and Uncinaria stenocephala. It is usually easy to diagnose, but the atypical presentation may occasionally mimic other dermatoses. Case report: A 32-year-old man presented with an extensive eczematous rash that developed during a recent vacation in Thailand. He didn’t respond to antihistamines and systemic steroids. Finally, he was diagnosed with an atypical presentation of CLM and treated successfully with anthelminthic therapy. Conclusion: The report of an atypical presentation of CLM is crucial to increase awareness among healthcare workers, helping in early diagnosis and reducing potential psychological distress that patients may face.
Orchestration of Photosynthesis-Associated Gene Expression and Galactolipid Biosynthesis during Chloroplast Differentiation in Plants
Authors: Fujii Sho , Wada Hajime , Kobayashi Koichi
Published: April 2024

The chloroplast thylakoid membrane is composed of membrane lipids and photosynthetic protein complexes, and the orchestration of thylakoid lipid biosynthesis and photosynthesis-associated protein accumulation is considered important for thylakoid development. Galactolipids consist of ∼80% of the thylakoid lipids, and their biosynthesis is fundamental for chloroplast development. We previously reported that the suppression of galactolipid biosynthesis decreased the expression of photosynthesis-associated nuclear-encoded genes (PhAPGs) and photosynthesis-associated plastid-encoded genes (PhAPGs). However, the mechanism for coordinative regulation between galactolipid biosynthesis in plastids and the expression of PhANGs and PhAPGs remains largely unknown. To elucidate this mechanism, we investigated the gene expression patterns in galactolipid-deficient Arabidopsis seedlings during the de-etiolation process. We found that galactolipids are crucial for inducing both the transcript accumulation of PhANGs and PhAPGs and the accumulation of plastid-encoded photosynthesis-associated proteins in developing chloroplasts. Genetic analysis indicates the contribution of the GENOMES UNCOUPLED1 (GUN1)–mediated plastid-to-nucleus signaling pathway to PhANG regulation in response to galactolipid levels. Previous studies suggested that the accumulation of GUN1 reflects the state of protein homeostasis in plastids and alters the PhANG expression level. Thus, we propose a model that galactolipid biosynthesis determines the protein homeostasis in plastids in the initial phase of de-etiolation and optimizes GUN1-dependent signaling to regulate the PhANG expression. This mechanism might contribute to orchestrating the biosynthesis of lipids and proteins for the biogenesis of functional chloroplasts in plants.
Sex on Steroids: How Brassinosteroids Shape Reproductive Development in Flowering Plants
Authors: Lima Rita B , Figueiredo Duarte D
Published: April 2024

Since the discovery of brassinolide in the pollen of rapeseed, brassinosteroids (BRs) have consistently been associated with reproductive traits. However, compared to what is known for how BRs shape vegetative development, the understanding of how these hormones regulate reproductive traits is comparatively still lacking. Nevertheless, there is now considerable evidence that BRs regulate almost all aspects of reproduction, from ovule and pollen formation to seed and fruit development. Here, we review the current body of knowledge on how BRs regulate reproductive processes in plants and what is known about how these pathways are transduced at the molecular level. We also discuss how the manipulation of BR biosynthesis and signaling can be a promising avenue for improving crop traits that rely on efficient reproduction. We thus propose that BRs hold an untapped potential for plant breeding, which could contribute to attaining food security in the coming years.
Photosynthetic control at the cytochrome b6f complex
Authors: Degen Gustaf E , Johnson Matthew P
Published: April 2024

Photosynthetic control (PCON) is a protective mechanism that prevents light-induced damage to PSI by ensuring the rate of NADPH and ATP production via linear electron transfer (LET) is balanced by their consumption in the CO2 fixation reactions. Protection of PSI is a priority for plants since they lack a dedicated rapid-repair cycle for this complex, meaning that any damage leads to prolonged photoinhibition and decreased growth. The imbalance between LET and the CO2 fixation reactions is sensed at the level of the transthylakoid ΔpH, which increases when light is in excess. The canonical mechanism of PCON involves feedback control by ΔpH on the plastoquinol oxidation step of LET at cytochrome b6f. PCON thereby maintains the PSI special pair chlorophylls (P700) in an oxidized state, which allows excess electrons unused in the CO2 fixation reactions to be safely quenched via charge recombination. In this review we focus on angiosperms, consider how photo-oxidative damage to PSI comes about, explore the consequences of PSI photoinhibition on photosynthesis and growth, discuss recent progress in understanding PCON regulation, and finally consider the prospects for its future manipulation in crop plants to improve photosynthetic efficiency.
New US deposit insurance rules for trust accounts take effect
Authors: Matusic Marla , Bisanz Matthew , Clements Ross , Leong Marc
Published: April 2024

On 1 April 2024, new requirements for US bank deposit insurance coverage for trust accounts took effect. These requirements apply to insured depository institutions, which include the over 4,500 US banks and similar organizations that are chartered under US law and hold deposits that are insured by the Federal Deposit Insurance Corporation. The new requirements apply retroactively to existing trust accounts, and some individuals may be entitled to more or less deposit insurance coverage than under existing requirements. Further, US-based banks that offer certificates of deposit (CDs), including structured CDs, to investors should be aware of how the new requirements affect their trust account clients’ deposit insurance and investment strategies and should update their program disclosures accordingly.
Can Discussions about Girls’ Education Improve Academic Outcomes? Evidence from a Randomized Development Project
Authors: Cotton Christopher S , Nordstrom Ardyn , Nanowski Jordan , Richert Eric
Published: April 2024

This article evaluates the impact that facilitated discussions about girls’ education have on education outcomes for students in rural Zimbabwe. The staggered implementation of components of a randomized education project allowed for the causal analysis of a dialogue-based engagement campaign. This campaign involved regular discussions between trained facilitators and parents, teachers, and youth about girls’ rights, the importance of attending school, and the barriers girls face in pursuing education. The campaigns increased mathematics performance and enrollment in the year after implementation. There was no similar improvement in literacy performance during this period. Longer-term data on the broader project suggest that adding additional education-focused interventions did not further increase mathematics performance and enrollment beyond what can be attributable to the dialogue campaigns alone.
A Multilocus Sequence Typing Scheme for Rapid Identification of Xanthomonas citri Based on Whole-Genome Sequencing Data
Authors: Okoh Efenaide B. , Payne Michael , Lan Ruiting , Riegler Markus , Chapman Toni A. , Bogema Daniel R.
Published: April 2024

Xanthomonas citri is a plant-pathogenic bacterium associated with a diverse range of host plant species. It has undergone substantial reclassification and currently consists of 14 different subspecies or pathovars that are responsible for a wide range of plant diseases. Whole-genome sequencing (WGS) provides a cutting-edge advantage over other diagnostic techniques in epidemiological and evolutionary studies of X. citri because it has a higher discriminatory power and is replicable across laboratories. WGS also allows for the improvement of multilocus sequence typing (MLST) schemes. In this study, we used genome sequences of Xanthomonas isolates from the NCBI RefSeq database to develop a seven-gene MLST scheme that yielded 19 sequence types (STs) that correlated with phylogenetic clades of X. citri subspecies or pathovars. Using this MLST scheme, we examined 2,911 Xanthomonas species assemblies from NCBI GenBank and identified 15 novel STs from 37 isolates that were misclassified in NCBI. In total, we identified 545 X. citri assemblies from GenBank with 95% average nucleotide identity to the X. citri type strain, and all were classified as one of the 34 STs. All MLST classifications correlated with a phylogenetic position inferred from alignments using 92 conserved genes. We observed several instances where strains from different pathovars formed closely related monophyletic clades and shared the same ST, indicating that further investigation of the validity of these pathovars is required. Our MLST scheme described here is a robust tool for rapid classification of X. citri pathovars using WGS and a powerful method for further comprehensive taxonomic revision of X. citri pathovars.
Temporal trend in mortality due to congenital heart disease in China from 2008 to 2021
Authors: Tian Youping , Hu Xiaojing , Gu Qing , Yang Miao , Jia Pin , Ma Xiaojing , Ge Xiaoling , Zhao Quming , Liu Fang , Ye Ming , Yan Weili , Huang Guoying
Published: April 2024

Background:

Congenital heart disease (CHD) is a leading cause of birth defect-related mortality. However, more recent CHD mortality data for China are lacking. Additionally, limited studies have evaluated sex, rural–urban, and region-specific disparities of CHD mortality in China.

Methods:

We designed a population-based study using data from the Dataset of National Mortality Surveillance in China between 2008 and 2021. We calculated age-adjusted CHD mortality using the sixth census data of China in 2010 as the standard population. We assessed the temporal trends in CHD mortality by age, sex, area, and region from 2008 to 2021 using the joinpoint regression model.

Results:

From 2008 to 2021, 33,534 deaths were attributed to CHD. The period witnessed a two-fold decrease in the age-adjusted CHD mortality from 1.61 to 0.76 per 100,000 persons (average annual percent change [AAPC] = −5.90%). Females tended to have lower age-adjusted CHD mortality than males, but with a similar decline rate from 2008 to 2021 (females: AAPC = −6.15%; males: AAPC = −5.84%). Similar AAPC values were observed among people living in urban (AAPC = −6.64%) and rural (AAPC = −6.12%) areas. Eastern regions experienced a more pronounced decrease in the age-adjusted CHD mortality (AAPC = −7.86%) than central (AAPC = −5.83%) and western regions (AAPC = −3.71%) between 2008 and 2021. Approximately half of the deaths (46.19%) due to CHD occurred during infancy. The CHD mortality rates in 2021 were lower than those in 2008 for people aged 0–39 years, with the largest decrease observed among children aged 1–4 years (AAPC = −8.26%), followed by infants (AAPC = −7.01%).

Conclusions:

CHD mortality in China has dramatically decreased from 2008 to 2021. The slower decrease in CHD mortality in the central and western regions than in the eastern regions suggested that public health policymakers should pay more attention to health resources and health education for central and western regions.