bluuebunny/crossref_metadata_2025 huggingface dataset:
Death by Lightning: Effective Public Health Strategies Needed
Authors: Nurunnabi Abu Sadat Mohammad , Islam Md Mozaharul , Sultan Mohammad Tipu , Sanwar Kursiya , Fatema Tofa Ishrak
Published: June 2022

Lightning is one of the leading causes of weather-related fatalities. A man, approximately 30 years old, was brought to the morgue of Kishoreganj District Hospital, Bangladesh, in July 2021, known to be lightning strike victim. During postmortem examination, various types of skin burns were found in the body, at an entry site on and near the head, and at an inferiorly located exit site. The skin injuries consisted of superficial burns, charring, and singed hair; however, there was no deep burn. „Lichtenberg figures‟ were found. Internal findings at autopsy were nonspecific. Epidemiologically, the total number of global annual lightning fatalities range from 6,000 to 24,000 per year. In recent years, Bangladesh experiences a high rate of lightning deaths. We feel that the number of deaths by lightning should be seen as a call to action. Hence, deaths by lightning should be regarded as a public health problem in the country and necessary measures must be taken. Our case study aims to draw the attention of the physicians and public health department on injuries and deaths due to lightning strike. It is also important for forensic professionals to be familiar with the nature of injuries caused by lightning and lightning strike deaths.

CBMJ 2022 January: vol. 11 no. 01 P: 52-55
A Young Bangladeshi Boy with Primary Ciliary Dyskinesia (Kartagener’s Syndrome): A Rare Case
Authors: Bari Muhammad Abdul , Hasan Mahmud Javed , Ahmed Sultan
Published: June 2022

Kartagener’s syndrome, an autosomal recessive inherited disorder, is a subgroup of primary ciliary dyskinesias (PCD). This genetic disorder manifests from early life which distinguishes it from acquired mucociliary disorders. Kartagener’s syndrome presents as a classical triad of situs inversus, sinusitis and bronchiectasis occurring majorly due to impaired ciliary motility. A 16 year-old boy from Fulbaria, Mymensingh with left sided consolidation for 1 month and recurrent episodes of nasal congestion and lower respiratory tract infection (LRTI) since his childhood. Clinical and imaging findings revealed left sided consolidation, chronic sinusitis, bronchiectasis, dextrocardia, and situs inversus. He was treated with orally administered antibiotics, bronchodilators, mucolytics, and chest physiotherapy. He was symptomatically better with the above therapy, and started on a long-term low-dose prophylactic antibiotic. As there is no easy, reliable non-invasive diagnostic test for Kartagener’s syndrome and the correct diagnosis is often delayed by years, it may cause chronic respiratory problems with reduced quality of life. Genetic counseling and fertility issues should be addressed once Kartagener’s syndrome is diagnosed.

CBMJ 2022 January: vol. 11 no. 01 P: 56-60
Modern Trends in the Formation and Development of the Human Rights Mechanism in Ukraine
Authors: Stefanchuk Maryna
Published: June 2022

The article highlights the modern determinants of the formation and function of institutions of the national human rights mechanism in Ukraine. Particular attention is paid to the institutions of the justice system as key elements of the national human rights mechanism, the formation and functioning of which, at the present stage, are determined by a number of factors, at both internal (national) and external (supranational) levels. It is established that external determinants determine the impact on the human rights mechanism in Ukraine through functional indicators of its effectiveness in the evaluation and reporting documents of the institutions of the supranational (international) human rights mechanism. Internal determinants dictate the impact on the national human rights mechanism through functional indicators of its effectiveness in the evaluation and reporting documents of national human rights institutions, the results of sociological research, and expert assessments and depend directly on the ‘quality of law’. The current trends in the development of human rights mechanisms in Ukraine, which are enshrined in a number of corresponding strategies in the field of human rights due to the need to improve the state’s activities to promote and ensure human rights and freedoms, create effective mechanisms for their implementation and protection in the field of development of the justice system as a whole, as well as its constituent institutions, such as the prosecutor’s office and the bar. Emphasis is placed on the priority of reaching consensus among stakeholders in the implementation of these strategic documents as a normative component, which determines the development trends of the institutional and functional components of the national human rights mechanism.
PENGATURAN PENGGUNAAN LAHAN DI DAERAH HULU DAS CIMANUK SEBAGAI UPAYA OPTIMALISASI PEMANFAATAN SUMBERDAYA AIR
Authors: Susetyaningsih Adi
Published: June 2022

Sungai Cimanuk merupakan salah satu sungai besar yang ada di wilayah propinsi Jawa Barat. DAS Cimanuk merupakan salah satu penopang utama sumberdaya air di Jawa Barat. Meningkatnya lahan kritis di daerah hulu DAS Cimanuk dapat mengancam kelangsungan Sungai Cimanuk sebagai salah satu sumberdaya air di Jawa Barat ini. Mengingat DAS sebagai satu kesatuan ekosistem maka perencanaanÂ  penggunaan lahan yang utuhÂ  sangat penting dan merupakan konsekuensi logis untuk menjaga kesinambungan pemanfaatan DAS Cimanuk sebagai sumberdaya air di propinsi Jawa Barat. Upaya untuk menyelamatkan hulu DAS Cimanuk dapat dilakukan melalui pendekatan administrative maupun teknis. Pendekatan adminstratisÂ  dilakukan dengan kebijakan pengawasan yang ketat terhadap perubahan tata ruang di daerah hulu. Disamping itu perlu pula dibentuk komunitas-komunitas dari masyarakat di sekitar DAS yang bisa mengelola lahan kritis menjadi lahan konservasi yang produktif.Â  Secara teknis upayaÂ  dilakukan dengan membangun cekdam di sepanjang DAS yang berfungsi menampung lumpur yang terbawa arus sungai. Lumpur atau kerikil yang tertampung di cekdam tersebut nantinya boleh dimanfaatkan warga, untuk dijual. Dengan demikian, limbah alam itu memiliki nilai ekonomi. Keterkaitan faktor penggunaan lahan dengan kemampuan sungai menyediakan sumberdaya air adalah kemampuannya dalam memberi sanggahan (buffer) terhadap masukan atau resapanÂ  curah hujan sehingga tidak menimbulkan sedimentasi dan debit air yang berlebih (banjir) akibat besarnya aliran permukaan
STUDI PEMANFAATAN RUANG PUBLIK UNTUK LAHAN PARKIR DI JALAN CIKURAY GARUT
Authors: Ibrahim Devi Maulana , Walujodjati Eko , Ismail Agus
Published: June 2022

Bentuk pemanfaatan ruang publik Kota Garut salah satunya adalah parkir khususnya Jalan Cikuray Garut. Tujuan penelitian ini untuk mengetahui karakter dan ruang publik, mengetahui karakter dan Â lahan parkir di ruang publik, dan menyusun arahan penataan lahan parkir di ruang publik. Metode penelitianya adalah observasi, wawancara, inventarisasi, dokumentasi. Teknik analisis yang digunakan adalah analisis deskriptif. Distribusi ruang publik di Kota Garut mengelompok di pusat kota dengan fungsi sebagai kawasan komersial, sebagai pusat kuliner dan sarana rekreasi. Pengembangan lahan parkir pada ruang publik didukung oleh Dinas Perhubungan, dan pengelolaan lahan parkir oleh masyarakat lokal. Hasil terakhir penelitian ini adalah penambahan serta pengadaan tempat khusus parkir kendaraan, pengadaan legalitas pengelola parkir, pemberdayaan terhadap petugas parkir legal, dan menjadikan lokasi ruang publik sebagai area parkir.
ANALISIS KARAKTERISTIK PARKIR PADA BADAN JALAN DAN DAMPAKNYA TERHADAP LALU LINTAS (STUDI KASUS: JALAN SILIWANGI KABUPATEN GARUT)
Authors: Wahdan Yaumil , Permana Sulwan
Published: June 2022

Salah satu ruas jalan yang tinggi aktivitasnya di Kabupaten Garut adalah Jalan Siliwangi. Jalan tersebut tidak hanya berfungsi sebagai pelayanan akses tetapi dijadikan pula sebagai fungsi mobilitas sebagai tempat parkir pada badan jalan (on street parking). akibatnya dapat menimbulkan permasalahan lalu lintas pada kawasan tersebut. Penelitian ini bertujuan menganalisis karakteristik parkir dan kebutuhan parkir serta mengetahui dampaknya terhadap lalu lintas. Data yang diperlukan meliputi data primer dan data sekunder dengan metode pengumpulan data pencatatan langsung nomor polisi kendaraan yang masuk dan keluar di lokasi studi pada 3 interval waktu, jam 07.00-09.00, 11.00-13.00, 15.00-17.00 dalam jangka waktu 3 hari yaitu, Minggu, Senin, dan Kamis. Hasil kajian menjelaskan bahwa, selama penelitian volume puncak tertinggi kendaraan ringan sebanyak 43 kendaraan dan sepeda motor 439 kendaraan dengan akumulasi maksimum 184 kendaraan. Durasi parkir maksimum kendaraan ringan 1,448 jam/kendaraan dengan parking turnover 2,182 mobil/petak dan sepeda motor 1,017 jam/kendaraan dengan parking turnover 4,722 motor/petak. Kapasitas parkir maksimum kendaraan ringan sebanyak 28 petak dan sepeda motor 123 petak. Indeks parkir maksimum kendaraan ringan sebesar 434,36%, dan untuk sepeda motor 944,33%. Jumlah petak parkir yang diperlukan yaitu 25 petak parkir kendaraan ringan dan 135 petak parkir sepeda motor, dan dampak yang ditimbulkannya yaitu pengurangan kapasitas ruas jalan dan kemacetan.
Program Pelatihan Web Development untuk Komunitas Maya
Authors: Ade Sutedi  , Dzikri Nursyaban  , Cahya Mutiara  , Dede Sopiah  , Diaz Radhian Salam  , Diva Nuratnika Rahayu  , Hasfi Syahrul Ramadhan  , Ilham Muhamad Ramdan  , Intan Sri Fatmalasari  , Irsyad Ahmad
Published: June 2022

Di era serba digital seperti saat ini, web sudah tidak asing lagi di telinga masyarakat. Bahkan, profesi sebagai Web Developer banyak diminati oleh kaum muda yang ingin menjadi freelancer. Oleh sebab itu, banyak kaum muda terutama siswa dan mahasiswa yang kini mulai tertarik untuk mempelajari Web Development. Program pelatihan ini bertujuan untuk memberikan pembelajaran dasar mengenai Web Development bagi pemula atau masyarakat umum. Metode pelaksanaanya dilakukan secara daring dengan mengadakan sesi webinar setiap minggu dan pembekalan modul secara berkala disertai tugas project yang berlangsung dalam kurun waktu satu bulan. Hasil yang dicapai yaitu dengan meningkatnya pemahaman dan kemampuan para peserta dalam membuat web sederhana.
Predictors of renal scars in infants with recurrent febrile urinary tract infection: a retrospective, single-center study
Authors: Han Jae Ha , Rhie Seonkyeong , Lee Jun Ho
Published: June 2022

Purpose: To determine predictive factors for detecting renal parenchymal damages (RPDs) in infants with recurrent febrile urinary tract infection (fUTI).Methods: From January 2015 to December 2021, 102 infants with recurrent fUTI and who underwent 99mTc-dimercaptosuccinic acid (DMSA) renal scan in our hospital were included in this study. Controls included infants with normal DMSA results performed 3 months apart from the 2nd episode of fUTI. DMSA-positive group included infants with positive DMSA results performed 3 months apart from the 2nd episode of fUTI or at the 3rd episode of fUTI. The recurrence rate, causative bacteria, renal size discrepancy of both kidneys, and laboratory findings including C-reactive protein (CRP) and spot urine sodium-to-potassium ratio (uNa/K) were compared between both groups.Results: Only 3.8% of 79 infants with a 2nd episode of fUTI showed positive DMSA results. fUTI recurred more frequently within 12 months of follow-up in the DMSA-positive group than in the control group (69% vs. 13%, P=0.00). CRP values were significantly higher in the DMSA-positive group than in the control group (7.3 mg/dL vs. 3.7 mg/dL, P=0.00). Spot uNa/K were significantly lower in the DMSA-positive group than in the control group (0.6 vs. 1.1, P=0.00). Conclusions: Congenital renal scar and RPDs on the DMSA scan were more frequently found in infants with recurrent fUTI than those in the control group. High CRP values and low spot uNa/K in acute infections were helpful in predicting the presence of RPD in infants with recurrent fUTI.
Clasificador automático de clase (adulto-cría) mediante características distintivas en vocalizaciones de manatíes
Authors: Morales Jaira , Pacheco Hazel , Merchan Spiegel Fernando
Published: June 2022

En este documento se desarrollan algunos métodos específicos para llevar a cabo un clasificador que sea capaz de identificar manatíes adultos y cría de distintos audios. La duración de las vocalizaciones se evaluó con un método de eliminación de ruido basado en eliminación de silencios, para luego aprovechar esta señal y evaluar uno de los principales parámetros, la frecuencia fundamental la cual se calculó con dos métodos (cepstrum y THD) y de esta manera se compararon los valores obtenidos y se evaluaron en un clasificador de mínima distancia.
Fabricación de polímeros a base de almidón de tubérculos panameños seleccionados
Authors: Rodríguez José , Montenegro Virna , Marín Nacarí
Published: June 2022

El alto índice de contaminación plástica en la actualidad ha llevado a la búsqueda de soluciones que sean amigables con el ambiente. Se presenta un estudio de la fabricación de polímero biodegradable a base de almidón de tubérculos panameños seleccionados (yuca, ñame, otoe y ñampí) y su evaluación mediante un análisis de tensión y biodegradación. Se generó un procedimiento para la extracción de almidón de los tubérculos, así como de la fabricación de biopolímero. El ensayo de tensión se hizo de manera artesanal con una probeta ASTM D638-14 tipo IV incrementando progresivamente el peso y registrando fotográficamente hasta su fractura. El ensayo de biodegradación se monitoreaba la presencia de hongos, se hizo un ensayo con una solución con enzimas digestivas que contenía amilasa, registrando su desintegración en 24 horas y por último, se sembraron los segmentos directamente al suelo y se registró su descomposición después de 7 y 15 días. Se pudo extraer el almidón y fabricar biopolímeros de todos los tubérculos mediante la metodología presentada. La acuosidad del almidón decantado variaba según el tubérculo. El biopolímero generado por ambos fue muy similar y dos de los óptimos para fabricar y cortar. En los ensayos de tensión el biopolímero de otoe mostro mayor resistencia al esfuerzo y deformación previa a la fractura comparado con los demás tubérculos y biopolímero de almidón de maíz utilizando la misma metodología de fabricación. Todos los biopolímeros mostraron avances en su proceso de biodegradación.
Efectos del acoso sexual callejero sobre el desarrollo del miedo y la ansiedad en mujeres entre los 20 y 30 años en Panamá
Authors: Gómez Lourdes , Álvarez Yuliana , Pauli Elianne , Oviedo Diana
Published: June 2022

Objetivos: Esta investigación tiene como propósito explorar los efectos derivados de la experimentación de acoso sexual callejero (ASC) en mujeres entre los 20 y 30 años en Panamá. Metodología: Para obtener resultados sobre la experimentación de ASC, mujeres entre los 20 y 30 años (N=33) contestaron un cuestionario sociodemográfico y la Escala de Ansiedad de Hamilton. A 5 de las participantes se les realizó una entrevista a profundidad para conocer sus experiencias de ASC y el efecto que estas han tenido sobre ellas. Resultados: Se encontró que todas las participantes del estudio (100%) reportaron haber experimentado miedo durante la experiencia de ASC. Otras emociones reportadas fueron: impotencia, rabia y enojo. Se evidenciaron asociaciones significativas (p = 0.01) entre las burlas de los acosadores ante las reacciones de las víctimas y la ansiedad. Se encontró que, al experimentar mayores niveles de ansiedad, las víctimas perciben cambios corporales y tienden a cambiar de vestimenta y sus rutas.  Conclusiones: El presente estudio es uno de los primeros en explorar en Panamá la experimentación del miedo y la ansiedad como un efecto directo del ASC. El acoso tiene repercusiones a nivel físico, social y emocional, por lo que debe comenzar a llamar la atención de la sociedad como una señal alarmante de la necesidad de educar a la población desde tempranas edades sobre el valor y el respeto que merece la mujer, como ser humano, en todos sus entornos y espacios de desarrollo.
SISTEM PENGAIRAN OTOMATIS MEDIA TANAM HIDROPONIK MENGGUNAKAN SENSOR KELEMBABAN UDARA
Authors: Hidayatulloh Syarif , Rifa’i Fahmi Alwi , Prasetya Rangga Ziancy
Published: June 2022

Population growth has led to a reduction in urban agricultural land. With the development of technology, the agricultural sector is also experiencing growth in farming patterns without soil media known as hydroponics. Hydroponics requires watering through pipes run by manually operated electric pumps. Human resources are needed to standby to operate it. Do not rule out the possibility, humans can be negligent and cause the water system is disrupted so that plants die. Making a pump active automatically is a way to reduce the occurrence of these problems. This automatic pumping system will be controlled by a microcontroller to irrigate plants automatically and can meet the required nutrient sources. An ATMega16 microcontroller is used that is programmed to analyze the input and output signals as needed. The input is a DHT11 sensor that is used to measure temperature and humidity, while the output is a water pump to irrigate all pipes. The output is also equipped with a 16x2 LCD which functions to display information on temperature and humidity. With the automatic irrigation system in hydroponic plants can save energy and facilitate the work in caring for plants with hydroponic growing media.
PERANCANGAN SISTEM INFORMASI PENDAFTARAN UMROH BERBASIS WEB PADA NURRAHMA TOUR & TRAVEL
Authors: Anwar Syaiful , Hilpiah Dida
Published: June 2022

Nurrahma Tour & Travel perwakilan Bogor adalah biro jasa perjalanan umroh, dimana perusahaan ini dalam melayani pendaftaran paket umroh mengalami kesulitan dalam pengolaan data pesertanya. Masalah yang terjadi pada sistem saat ini, calon jamaah umroh masih melakukan pendaftaran secara manual seperti mengisi formulir, pencarian data jamaah masih dicari secara manual sehingga tidak efisien, dan pembuatan laporan daftar jamaah yang cukup lama karena harus mengumpulkan data secara keseluruhan. Maka salah satu solusi pemecahan masalah tersebut dengan membuat aplikasi pendaftaran umroh. Metode penelitian ini merupakan studi kasus perancangan aplikasi online menggunakan model waterfall yang terdiri dari lima tahapan pembahasannya disertai juga dengan LRS dan Navigasi. Tujuan dari penelitian ini ingin mengimplementasikan sistem pendaftaran umroh secara online kepada para calon jamaah untuk meningkatkan efektifitas bagi calon jamaah maupun perusahaan itu sendiri..  Dan hasil dari penelitian ini berupa rancangan aplikasi pendaftaran umroh  yang dapat dijalankan secara online sehingga memudahkan dalam melakukan pendaftaran umroh dan pengelolaan datanya.
RANCANG BANGUN APLIKASI SISTEM INFORMASI ABSENSI KARYAWAN ONLINE
Authors: Roosdianto Rully , Sari Ani Oktarini , Satriansyah Arief
Published: June 2022

Absensi dan rekap absensi adalah kegiatan yang sangat penting dalam proses penggajian karyawan. Perhitungan absen yang dihitung dengan jumlah hari kerja menjadikan proses ini perlu adanya pengawasan dalam pencatatan data absensi maupun perekapannya nanti. CV Cahaya Toner adalah perusahaan swasta yang bergerak dibidang retail toner dan printer, yang sudah berdirikan sejak akhir tahun 2014. Sistem yang digunakan dalam proses absensi karyawan pada CV Cahaya Toner masih manual, yakni dengan dicatat buku absensi dan membubuhkan tanda tangan. Hal ini tentu saja memiliki dampak pada efektifitas dan efisiensi pendataan, pencarian data, dan menghitung rekap absensi yang membutuhkan waktu, sehingga resiko kesalahan akan kehilangan data absensi semakin besar. Untuk itulah dibutuhkannya aplikasi yang digunakan untuk absensi dan sekaligus monitoring dalam rekapan absensinya. Metode penelitian yang digunakan adalah pengembangan dengan model waterfall. Diharapkan dengan adanya aplikasi absensi karyawan berbasisw web ini dapat memberikan kemudahan dalam proses absensi, pencarian data dan perhitungan rekap absensi, serta mengurangi resiko kehilangan dan kesalahan pencatatan data absensi pada CV Cahaya Toner.
PERANCANGAN APLIKASI LAYANAN SALON HEWAN SECARA DARING MENGGUNAKAN MODEL PROSES INCREMENTAL
Authors: Wasesha Dian Ambar
Published: June 2022

Happy Petshop merupakan salon hewan yang memiliki layanan seperti gunting kuku, mandi dan beberapa terapi untuk hewan peliharaan agar lebih sehat. Pandemi yang cukup lama mempengaruhi jumlah kunjungan pelanggan Happy Petshop menjadi semakin sedikit karena pergerakan masyarakat sangat dibatasi. Sedangkan pemesanan layanan pada Happy Petshop masih menggunakan sistem konvensional, dimana para pelanggan harus datang langsung ke outlet dengan membawa hewan peliharaan mereka. Untuk itu inovasi harus dilakukan oleh manajemen Happy Petshop agar bisnis tetap berjalan. Mengkonversikan layanan pemesanan grooming dari luring menjadi daring adalah solusi yang dipilih. Ditambah dengan fasilitas antar jemput agar pelanggan tetap aman dirumah menghidari terinfeksi dari covid 19. Pengembangan sistem ini dibuat dengan model proses incremental. Model proses incremental ini berfokus pada pengiriman produk di tiap increment-nya. Dengan adanya sistem ini diharapkan Happy Petshop dapat menarik pelanggan lebih banyak dengan jangkauan yang lebih luas. Dan dapat memberikan kenyamanan dan kemudahan bagi pelanggannya.
Transient hypobaric hypoxia improves spatial orientation in young rats.
Authors: Wittner M , Říha P
Published: June 2022

To achieve a better understanding of learning and declarative memory under mild transient stress, we investigated the effect of brief hypobaric hypoxia on spatial orientation in rats. Young male Wistar rats aged 30 days were exposed for 60 min to hypobaric hypoxia, simulating an altitude of 7,000 m (23,000 ft) either shortly prior to attempting or after mastering an allothetic navigation task in the Morris water maze with a submerged platform. The post-hypoxic group performed significantly better in the navigation task than the control animals (the mean difference in escape latencies was 11 seconds; P=0.0033, two-way ANOVA with repeated measures, group x session). The experimental group also achieved a remarkably higher search efficiency (calculated as a percentage of successful trials per session), especially during the first four days following hypoxic stress (P=0.0018). During the subsequent training, the post-hypoxic group performed better than the control animals, whilst the efficiency levels of both groups progressively converged. Spatial memory retention and recall of well-trained rats were not affected by the transient hypobaric hypoxia. These results indicate that brief hypobaric hypoxia enhances rats' spatial orientation. Our findings are consistent with several studies, which also suggested that mild transient stress improves learning.
The postoperative stress response and its reflection in cytokine network and leptin plasma levels.
Authors: Chachkhiani I , Gürlich R , Maruna P , Fraško R , Lindner J
Published: June 2022

The objective of the present report was to clarify the postoperative stress response of some inflammatory markers, namely of proinflammatory cytokines and leptin levels during uncomplicated postoperative periods. The results were compared with the dynamics of these parameters during intraabdominal sepsis. We followed 20 patients after a planned resection of colorectal cancer in stage Ib-IV with uncomplicated healing and 13 obese men after laparoscopic non-adjustable gastric banding. These were compared to 12 patients with proven postoperative sepsis. The control group consisted of 18 healthy men. The observed parameters included serum levels of cytokines, tumor necrosis factor-alpha (TNFalpha), interleukin-1 beta (IL-1 beta), interleukin-1 receptor antagonist (IL-1 ra), IL-6, IL-8, soluble receptor of interleukin-2 (sIL-2R) and leptin. It was found that during the first 24 h after resection there was a significant increase in the serum concentration of IL-6 up to 1125+/-240 ng/l, which declined within the next 48-72 h. Serum concentration of TNFalpha was highest 18-24 h after resection (205+/-22 ng/l) and after banding (184+/-77 ng/l). IL-1 beta had a stable serum concentration without significant elevation. Serum concentration of IL-8 after resection rose to 520+/-200 ng/l after 36-48 h. Maximal cytokine levels after gastric banding were quantitatively lower (IL-6 414+/-240 ng/l, TNFalpha 184+/-77 ng/l) than after resection. We found significant elevation of plasma leptin concentration (32+/-10 ng/ml) 24 h after banding compared with preoperative values (18+/-5 ng/ml, p 0.05). Leptin levels 48 and 72 h after banding rapidly returned to the level before operation. During abdominal surgery leptin shows to be an acute phase reactant. Proinflammatory cytokines can be main regulatory factors of leptin during this period. Significant correlation between leptin and TNFalpha (similarly demonstrated by other authors in models of bacterial inflammation) indicates that TNFalpha can be the crucial regulator of leptin generation in the early postoperative period. On the basis of our results we recommend to observe IL-6 and IL-8 at 24-72 h after the surgery in patients with a high risk of early postoperative septic complications.
Effect of systemic hypoxia and reoxygenation on electrical stability of the rat myocardium: chronophysiological study.
Authors: Švorc P , Beňačka R , Petrášová D , Bračoková I , Kujaník Š
Published: June 2022

The aim of the study was to determine the dependence of changes in the electrical stability of the heart on the light-dark cycle (LD cycle) in disorders of pulmonary ventilation. The ventricular arrhythmia threshold (VAT) was measured in female Wistar rats (adaptation to the light regime 12:12 h, ketamine/xylazine anesthesia 100 mg/15 mg/kg, i.m., open chest experiments). The conditions of the normal artificial ventilation and reoxygenation were V(T) = 1 ml/100 g, respiratory rate 40 breaths/min, hypoventilation V(T) = 0.5 ml/100 g, respiratory rate 20 breaths/min. The animals (n=11 light group; n=19 dark group) were subjected to 20 min hypoventilation followed by 20 min reoxygenation. The control prehypoventilatory VAT differences were not found between the light (1.90+/-0.84 mA) and dark (1.88+/-0.87 mA) part of the day. Artificial hypoventilation changed the VAT values in light and dark part of the day differently. While during the light period, the average VAT values in most animals (90.9 %) were significantly decreased (1.29+/-0.59 vs. 1.90+/-0.84 mA control, p<0.05), during the dark part these values showed either significant increase (63.2 %) (2.23+/-0.77 vs. 1.48+/-0.39 mA, p<0.005) or a slight non-significant decrease (36.8 %) (2.18+/-0.89 vs. 2.54+/-0.99 mA). Reoxygenation returned the VAT values to the level before hypoventilation by an increase of the VAT (81.8 %) in the light part of day and by decrease of the VAT (68.4 %) in the dark part of the day. It is concluded that 1) in hypoventilation/reoxygenation model, the significant higher average VAT values are in the dark part of the day vs. the light one, 2) rat hearts are more resistant to systemic hypoxia and reoxygenation in the dark part of day, and 3) proarrhythmogenic effect of the systemic hypoxia is only seen in the light part of the day.
LEARNING INDEPENDENCE OF BIOLOGICAL EDUCATION STUDENTS IN CALCULUS COURSE ONLINE REVIEWED BY GENDER
Authors: Silitonga R. H. Yanti
Published: June 2022

This research was conducted to determine the independence of student learning in calculus courses conducted online based on gender. This type of research is descriptive quantitative. The subjects in this study were 34 biology education students. There are 4 male students and 30 female students. Methods of data collection using a scale of learning independence. The results showed that there were significant differences in the learning independence of male and female students. Female students outperform male students for all indicators of independent learning. The percentage of the average score of male students 'learning independence was 59.37, while the percentage of the average score of female students' learning independence reached 66.54.
 
Keywords :
THE APPLICATION OF PROBLEM-BASED LEARNING MODELS TO IMPROVE MATHEMATICS LEARNING OUTCOMES FOR STUDENTS OF CLASS XII IPA 6 SMA NEGERI 4 PEKANBARU
Authors: Aries Citra
Published: June 2022

This classroom action research aims to improve the learning process and improve mathematics learning outcomes by applying problem-solving strategies through problem-based learning. The subjects of this study were students of class XII IPA 6 SMA Negeri 4 Pekanbaru, totaling 38 students with academic ability levels heterogeneous. This study consisted of two cycles consisting of four stages, namely planning, implementation, observation, and reflection. Data collection was carried out through observations of teacher and students activities and learning outcomes tests in daily tests. There is an improvement towards a better direction in the learning process after problem-solving strategies through problem-based learning. This can be seen in students who participate actively and are more responsible in the learning process, such as discussing, presenting, expressing opinions, and asking questions to provide a learning conclusion. The number of students who achieved the KKM on the basic score, daily test I (first cycle), daily test II (second cycle) was 47,36%, 65,78%, and 92,10%, respectively. The percentage of KKM achievement also shows an increase from before to after the action, so that it can be said that student learning outcomes also increase. The results of this study indicate that the application of problem solving strategies through problem-based learning can improve the learning process and improve mathematics learning outcomes for students of grade XII science 6 SMA Negeri 4 Pekanbaru.
DEVELOPMENT OF MATHEMATICS LEARNING DEVICES BASED ON PROBLEM-BASED LEARNING ON THE MATERIAL OF POLYHEDRON FOR CLASS VIII SMP/MTs STUDENTS
Authors: Siahaan Evi Afrina Widayati , Armis
Published: June 2022

This research aims to produce mathematics learning tools in the form of syllabus, lesson plans (RPP), and student worksheets (LKPD) based on learning models based on the problem of building flat side spaces for 8th-grade students of junior high school. This research uses a 4-D development model which includes four stages, namely: (1) define (defining); (2) design (planning); (3) develop (development); and (4) disseminate (spread). The research instrument used is the instrument of validity and practicality. The validity instrument is in the form of a validation sheet to assess the feasibility of the syllabus, lesson plans, and LKPD. The practical instrument is in the form of a student response questionnaire to the use of LKPD. The learning tools that have been compiled are then validated by three validators and revised according to suggestions from the validators. The valid learning tools were then tested in small groups with the subject of 6 students in grade 8th junior high school. Based on the data analysis and discussion results, mathematics learning tools in the form of Syllabus, RPP and LKPD based on learning models based on problems in the flat-sided space building material are considered very valid and practical to be used by 8th-grade students of junior high school.
Siyer-Hadis İlişkisi Bağlamında Selmân-ı Fârisî’nin Müslüman Oluşu
Authors: Topal Ramazan
Published: June 2022

Hz. Peygamber’in hayatını ele alan siyer, İslâmî ilimler arasında en fazla hadis ile ortak noktalara sahiptir. Hatta bazı âlimler siyerin içerisinde yer aldığı tarih ilmini hadisin bir alt dalı olarak kabul etmişlerdir. Hadis eserlerinde zengin bir siyer malzemesi bulunması ve Zührî ile İbn İshâk gibi müverrih râvilerin rivayetlerinin bu eserlerde nakledilmesi, iki ilmin aralarındaki bağı kuvvetlendirmiştir.
Siyer ve hadisin usul ve içerik bakımından birçok ortak noktaya sahip olmakla beraber birbirinden ayrıldıkları yönler de vardır. Meselâsiyerde senede, hadiste olduğu kadar önem verilmemektedir. Aynı şekilde hadiste olayların tarihi ve mekânını tespit etmek, temel hedeflerden biri değildir. Çünkü her iki ilmin farklı gaye ve yöntemleri bulunmaktadır. Dolayısıyla bu iki alana hitap eden çalışmalarda bunlara dikkat edilmelidir.
Bu makalede siyer-hadis irtibatının bir örneği olmak üzere Selmân-ı Fârisî’nin İslâm’ı kabul edişine dair Kütüb-i Tis‘a’da yer alan bilgiler, ilk dönem siyer kaynaklarıyla mukayese edilerek değerlendirilmiştir. Özellikle hem müverrih hem de muhaddis olan İbn İshâk’ın her iki alandaki rivayetlere yön vermesi dikkat çekmektedir.
DOES GASTRONOMY BRAND IMAGE AFFECT THE PERCEIVED EMOTIONAL VALUE AND TOUR SATISFACTION?
Authors: AYDIN Melih , SARI GÖK Hatice , SALVARCI Simge
Published: June 2022

Gastro-cultural tours, which are prevalent as part of today’s experience-based travel, generate memorable gastronomy encounters for tourists. Gastronomically designed cultural tours also shed light on the past's heritage and cultural identity through the local tastes of the regions by promising distinct stories and experiences to travelers. Regions with gastronomic affluence arouse gastro tourists’ interest with their cultural and tourist attractions. One of the critical factors contributing to the awareness of visitors towards a particular destination is the creation of a gastronomy brand image in their minds. A region with a gastronomy brand can also affect the emotional value tourists attribute to the region. From different perspectives, this cuisine-based brand image appears to have the potential to influence tourists' satisfaction with the tour. The Southeastern Anatolia Region, one of Turkey's most prominent culinary centers, is a diverse region with both indigenous gastronomic value and deep-rooted cultural history. From this point of view, the effects of gastronomy brand image on perceived emotional value and tour satisfaction of tourists taking part in gastro-cultural tours were examined in this study. According to the data obtained from the participants, gastronomy brand image has a significant effect on tour satisfaction and the perceived emotional value of the tourists.
Environmental Tax Convergence and Interdependence in the European Union
Authors: Ripollés Jordi , Vidal Meliá Lidia
Published: June 2022

This paper empirically analyzes the convergence and interdependence of environmental taxes in Europe from 1998 to 2018. Our results evidence a lack of European integration and reveal a group of converging countries characterized by a lowering tax burden. Moreover, a subsequent spatial regression analysis supports that the converging countries exhibit a significantly greater sensibility to the neighboring environmental tax policies, compatible with some degree of cross-country tax competition.
Same-Day Desensitization in Patients Who Experience Their First Reaction to a Platin Agent at the Oncology Day Unit: A Pilot Study to Safely Include This Technique Within the Multidisciplinary Pathways for the Diagnosis & Management of Hypersensitivity to Platin Agents
Authors: Borrás Cuartero Julián , Farzanegan Miñano Roxana , Torres Gorriz María Cruz , Germán Sánchez Adrián , Aznar Raquel Cervera , Raducan Isabela , Castelló Carrascosa Jose Vicente , Sanchez Hernandez Alfredo , Enrique Ernesto
Published: June 2022

One of the main objectives when assessing patients who react to antineoplastics must be to ensure that they receive the required treatments without delay. From January to July 2021, at the Allergy Department at the Provincial University Consortium Hospital a pilot study was performed in which those patients suspected of having suffered a type I hypersensitivity reaction (grade 1 or 2) following Brown's anaphylaxis severity grading to a platin agent at the Provincial University Consortium oncology day unit, and once the reaction was properly treated and completely resolved, were subjected to a new procedure named as Same-Day Desensitization, which consists in the reintroduction and administration of full chemotherapy dose by allergists on the same day of the reaction by following the 1 bag/10 step protocol, looking forwards to systematize same-day reexposure using Same-Day Desensitization, doing it in the safest way possible. In total, 9 oncological patients suspected of having suffered a type I hypersensitivity reaction (grade 1 or 2) to a platin agent received total dose administration the same day of the initial reaction by following Same-Day Desensitization 1 bag/10 step protocol, without presenting further reactions. The manuscript describes a new approach in the use of Rapid Drug Desensitizations in reactive oncologic patients in treatment with platin agents, presenting the first 9 cases of oncologic patients who have been submitted to this procedure.
A Gene Transfer-Positive Cell Sorting System Utilizing Membrane-Anchoring Affinity Tag
Authors: Yang Lele , Cui Lifang , Ma Shumin , Zuo Qingqing , Huang Qilai
Published: June 2022

Gene delivery efficiency is an essential limit factor in gene study and gene therapy, especially for cells that are hard for gene transfer. Here we develop an affinity cell sorting system that allows efficient enrichment of gene transfer-positive cells. The system expresses an enhanced green fluorescent protein (EGFP) fused with an N-terminal high-affinity Twin-Strep-Tag (TST) that will be anchored to the cell membrane at the out-surface through a glycosylphosphatidylinositol (GPI) membrane-anchoring structure. The EGFP permits microscopy and flow cytometry analysis of the gene transfer-positive cells, and the TST tag at the N terminal of EGFP allows efficient affinity sorting of the positive cells using Strep-Tactin magnetic beads. The cell sorting system enables efficient isolation of gene transfer-positive cells in a simple, convenient, and fast manner. Cell sorting on transfected K-562 cells resulted in a final positive cell percentage of up to 95.0% with a positive cell enrichment fold of 5.8 times. The applications in gene overexpression experiments could dramatically increase the gene overexpression fold from 10 times to 58 times, and in shRNA gene knockdown experiments, cell sorting increased the gene knockdown efficiency from 12% to 53%. In addition, cell sorting in CRISPR/Cas9 genome editing experiments allowed more significant gene modification, with an editing percentage increasing from 20% to 79%. The gene transfer-positive cell sorting system holds great potential for all gene transfer studies, especially on those hard-to-transfect cells.
On the Interaction Between SMARCAL1 and BRG1
Authors: Bisht Deepa , Patne Ketki , Rakesh Radhakrishnan , Muthuswami Rohini
Published: June 2022

SMARCAL1 and BRG1, both classified as ATP-dependent chromatin remodeling proteins, play a role in double-strand break DNA damage response pathways. Mutations in SMARCAL1 cause Schimke Immuno-osseous Dysplasia (SIOD) while mutations in BRG1 are associated with Coffin-Siris Syndrome (CSS4). In HeLa cells, SMARCAL1 and BRG1 co-regulate the expression of ATM, ATR, and RNAi genes on doxorubicin-induced DNA damage. Both the proteins are found to be simultaneously present on the promoter of these genes. Based on these results we hypothesized that SMARCAL1 and BRG1 interact with each other forming a complex. In this paper, we validate our hypothesis and show that SMARCAL1 and BRG1 do indeed interact with each other both in the absence and presence of doxorubicin. The formation of these complexes is dependent on the ATPase activity of both SMARCAL1 and BRG1. Using deletion constructs, we show that the HARP domains of SMARCAL1 mediate interaction with BRG1 while multiple domains of BRG1 are probably important for binding to SMARCAL1. We also show that SIOD-associated mutants fail to form a complex with BRG1. Similarly, CSS4-associated mutants of BRG1 fail to interact with SMARCAL1, thus, possibly contributing to the failure of the DNA damage response pathway and pathophysiology associated with SIOD and CSS4.
Role of SNAREs in Unconventional Secretion—Focus on the VAMP7-Dependent Secretion
Authors: Vats Somya , Galli Thierry
Published: June 2022

Intracellular membrane protein trafficking is crucial for both normal cellular physiology and cell-cell communication. The conventional secretory route follows transport from the Endoplasmic reticulum (ER) to the plasma membrane via the Golgi apparatus. Alternative modes of secretion which can bypass the need for passage through the Golgi apparatus have been collectively termed as Unconventional protein secretion (UPS). UPS can comprise of cargo without a signal peptide or proteins which escape the Golgi in spite of entering the ER. UPS has been classified further depending on the mode of transport. Type I and Type II unconventional secretion are non-vesicular and non-SNARE protein dependent whereas Type III and Type IV dependent on vesicles and on SNARE proteins. In this review, we focus on the Type III UPS which involves the import of cytoplasmic proteins in membrane carriers of autophagosomal/endosomal origin and release in the extracellular space following SNARE-dependent intracellular membrane fusion. We discuss the role of vesicular SNAREs with a strong focus on VAMP7, a vesicular SNARE involved in exosome, lysosome and autophagy mediated secretion. We further extend our discussion to the role of unconventional secretion in health and disease with emphasis on cancer and neurodegeneration.
Myosin Heavy Chain Converter Domain Mutations Drive Early-Stage Changes in Extracellular Matrix Dynamics in Hypertrophic Cardiomyopathy
Authors: Hsieh Jeanne , Becklin Kelsie L. , Givens Sophie , Komosa Elizabeth R. , Lloréns Juan E. Abrahante , Kamdar Forum , Moriarity Branden S. , Webber Beau R. , Singh Bhairab N. , Ogle Brenda M.
Published: June 2022

More than 60% of hypertrophic cardiomyopathy (HCM)-causing mutations are found in the gene loci encoding cardiac myosin-associated proteins including myosin heavy chain (MHC) and myosin binding protein C (MyBP-C). Moreover, patients with more than one independent HCM mutation may be at increased risk for more severe disease expression and adverse outcomes. However detailed mechanistic understanding, especially at early stages of disease progression, is limited. To identify early-stage HCM triggers, we generated single (MYH7 c.2167C > T [R723C] with a known pathogenic significance in the MHC converter domain) and double (MYH7 c.2167C > T [R723C]; MYH6 c.2173C > T [R725C] with unknown significance) myosin gene mutations in human induced pluripotent stem cells (hiPSCs) using a base-editing strategy. Cardiomyocytes (CMs) derived from hiPSCs with either single or double mutation exhibited phenotypic characteristics consistent with later-stage HCM including hypertrophy, multinucleation, altered calcium handling, metabolism, and arrhythmia. We then probed mutant CMs at time points prior to the detection of known HCM characteristics. We found MYH7/MYH6 dual mutation dysregulated extracellular matrix (ECM) remodeling, altered integrin expression, and interrupted cell-ECM adhesion by limiting the formation of focal adhesions. These results point to a new phenotypic feature of early-stage HCM and reveal novel therapeutic avenues aimed to delay or prohibit disease onset.
Cas-Based Systems for RNA Editing in Gene Therapy of Monogenic Diseases: In Vitro and in Vivo Application and Translational Potential
Authors: Reshetnikov Vasiliy V. , Chirinskaite Angelina V. , Sopova Julia V. , Ivanov Roman A. , Leonova Elena I.
Published: June 2022

Rare genetic diseases reduce quality of life and can significantly shorten the lifespan. There are few effective treatment options for these diseases, and existing therapeutic strategies often represent only supportive or palliative care. Therefore, designing genetic-engineering technologies for the treatment of genetic diseases is urgently needed. Rapid advances in genetic editing technologies based on programmable nucleases and in the engineering of gene delivery systems have made it possible to conduct several dozen successful clinical trials; however, the risk of numerous side effects caused by off-target double-strand breaks limits the use of these technologies in the clinic. Development of adenine-to-inosine (A-to-I) and cytosine-to-uracil (C-to-U) RNA-editing systems based on dCas13 enables editing at the transcriptional level without double-strand breaks in DNA. In this review, we discuss recent progress in the application of these technologies in in vitro and in vivo experiments. The main strategies for improving RNA-editing tools by increasing their efficiency and specificity are described as well. These data allow us to outline the prospects of base-editing systems for clinical application.
Huaier Induces Immunogenic Cell Death Via CircCLASP1/PKR/eIF2α Signaling Pathway in Triple Negative Breast Cancer
Authors: Li Chen , Wang Xiaolong , Chen Tong , Li Wenhao , Zhou Xianyong , Wang Lishui , Yang Qifeng
Published: June 2022

Triple-negative breast cancer (TNBC) is the most lethal breast cancer subtype owing to the lack of targeted therapeutic strategies. Immunogenic cell death (ICD), a modality of regulated cancer cell death, offered a novel option for TNBC via augmenting tumor immunogenic microenvironment. However, few ICD-inducing agents are currently available. Here, we showed that Trametes robiniophila Murr (Huaier) triggered ICD in TNBC cells by promoting cell surface calreticulin (CRT) exposure, and increasing release of adenosine triphosphate (ATP) and high-mobility group protein B1 (HMGB1). Co-culturing with Huaier-treated TNBC cells efficiently enhanced the maturation of dendritic cells (DCs), which was further validated via cell-based vaccination assay. In the xenograft mouse model, oral administration of Huaier led to tumor-infiltrating lymphocytes (TILs) accumulation and significantly delayed tumor growth. Besides, depletion of endogenous T cells obviously abrogated the effect. Mechanically, Huaier could elicit endoplasmic reticulum (ER) stress-associated ICD through eIF2α signaling pathway. Further studies revealed that circCLASP1 was involved in the Huaier-induced immunogenicity by binding with PKR in the cytoplasm and thus blocking its degradation. Taken together, we highlighted an essential role of circCLASP1/PKR/eIF2α axis in Huaier-induced ICD. The findings of our study carried significant translational potential that Huaier might serve as a promising option to achieve long-term tumor remission in patients with TNBC.
The Relationship Between Microbial Community and Breast Cancer
Authors: Song Xuelian , Wei Changran , Li Xiangqi
Published: June 2022

Breast cancer (BC) is the most common cancer in women and the leading cause of cancer-related deaths in women worldwide. Recent research studies have shown that the intestinal flora is related to the occurrence and progression of BC. Notably, some evidence identifies a unique microbial community in breast tissue, a site previously thought to be sterile. In addition, breast tumors have their own specific microbial community, distinct from normal mammary gland tissue, and all of them may result from intestinal flora. Some microbial community in breast tissue may lead to the occurrence and development of BC. This review focuses on the relationship between the microbial community and breast cancer, which will lay a solid theoretical foundation for further understanding the local microenvironment of BC and developing effective targeted therapeutic drugs.