bluuebunny/crossref_metadata_2025 huggingface dataset:
Removal of Ammonium Ions and Ammonia from Groundwater by Oxidation Processes
Authors: Lupascu Tudor , Ciobanu Mihai , Botan Victor , Sandu Ioan Gabriel , Dragalin Ion , Mitina Tatiana , Sandu Ion
Published: February 2020

The process of removal of ammonium ions and ammoniafrom ground water, which contained these components in quantities exceeding the maximum permissible limit, was studied by the oxidation method. It was shown by the CG-MS method and thin layer chromatography that calcium hypochlorite in certain amounts and at certain pH values oxidizes ammonium ions and ammonia, presumably with the formation of free nitrogen, without forming chloramines, reducing concentration of ammonium ions and ammonia to values below the maximum allowable limit for drinking water.
Role of Immunohistochemical Tests in Early Diagnosis of T-cell Lymphoma with Primary Oral Manifestations
Authors: Muica Adrian , Oltean Galafteon , Mocan Simona , Horvath Emoke , Grozescu Veronica , Muica Lucia Maria , Bechir Edwin Sever , Elsaafin Mahmoud , Pacurar Mariana
Published: February 2020

The immunohistochemistry investigations have an essential role in the establishment of the early diagnosis and in evaluating the management of T cell lymphoma disease with first localization in the oral-pharynx area. This study present the role of immunohistochemistry tests in the diagnosis of T cell lymphoma with first oral manifestation and detailed the case of a patient with T cell lymphoma located at the level of the palatine tonsils. Early diagnosis was followed by a treatment according to Protocol LSG 15 associated with bone marrow transplant, and the evolution being favorable.
The Role of Monoclonal Antibody Targeted Therapy in Uterine Sarcomas
Authors: Cirlan Cristian , Calin Cezar Ionut , Costea Radu , Barbilian Adrian Gheorghe
Published: February 2020

The uterine sarcoma is one of the type of neoplasm that affects the abdominopelvine organs. The behaviour of this hystopathological type of cellular line is very aggresive both to the primer organ and also to the nearby organs and to the main filter� organs like the liver and lung, where it develops metastasis. The treatment of this disease is multidisciplinary and it uses many therapeutic strategies depending on staging and the other comorbidities of the pacient. We will present the case of an 81 year old female known with the diagnosis of relapse tumoral abdominopelvin sarcoma developed one year after total hysterectomy surgery with bilateral anexetomie for the uterine sarcoma. Also we will discuss about a series of characteristics of the treatment plan observed at different similar cases. We will evaluate the latest drug lines used in oncology treatment, we will discuss about the therapeutic indications and in the end we will draw conclusions about the therapeutic indications of each case.
Researches on the Diffusion Phenomena in the Thermo-Chemical Treatment of Oxy-nitrocarburizing of Some Grey Cast Irons
Authors: Sicoe Gina Mihaela
Published: February 2020

The thermo-chemical treatment of oxy-nitrocarburizing consists in the enrichment of materials in C, N and O in order to improve the physico-mechanical properties and the use of the materials. This leads to the increase in exploitation life, the increase of wear resistance, galling, corrosion, and slight erosion. The research presented in this article followed the study of the application of this treatment and the results obtained on pearlitic grey cast iron with different chemical compositions, domain in which there are few experimental results published. The results obtained on a number of 10 samples, presented in the article, highlighted the good compatibility of these materials with the thermo-chemical treatment of oxy-nitrocarburizing, obtaining on the surface some white layers which give good anti-corrosive properties, similar to those obtained on steel. There was no correlation between the depth of the white layer and the chemical composition of the studied cast iron. It was also emphasized that the depth of nitrogen diffusion is influenced by the chemical composition of the studied materials which is directly proportional to carbon equivalent (Ceq) of cast iron.
Study of the Total Serum Concentration of Serrum Ionized Magnesium in Children and Adolescents from Sibiu Area
Authors: Antonescu Elisabeta , Bota Gabriela , Serb Bogdan , Atasie Diter , Tataru Cristina Dahm , Totan Maria , Duica Lavinia , Silisteanu Sinziana Calina , Szakacs Julianna , Arghir Oana Cristina , Oswald Ioan , Manea marinela Minodora
Published: February 2020

Magnesium is an essential nutrient for the living organisms and plays an important part in the prevention and treatment of many diseases. It is an enzymatic cofactor for more than 300 reactions. Magnesium is essential for regulating blood pressure, muscle contraction, cardiac excitability, insulin metabolism, vasomotor tonus. Studying the way in which serum magnesium concentration varies in children and adolescents in the Sibiu area according to the reference intervals we especially set for this area. The study is a retrospective one, using approximately 4900 data from the archives of the Medical Analysis Laboratory within the Sibiu Pediatric Clinical Hospital. Serum magnesium was dosed using the Konelab Prime 30i analyser. The data from the literature was used to compare the results. The reference ranges obtained in the current study were similar to the literature studied. The percentage of patients with magnesium concentration outside the reference ranges was roughly equal for all age groups. The difference was between 1 month and 2 year-old children with very few deviations from the reference range. The results of our study reflect more accurately the real reference range for the population in the Sibiu area, helping clinicians to establish a diagnosis as quickly and accurate as possible. These results were not significantly different from the literature studied.
Design and Synthesis of the Candesartan Key Intermediate
Authors: Pintilie Lucia , Stefaniu Amalia , Negut Catalina , Tanase Constantin , Caproiu Miron Teodor
Published: February 2020

This paper presents experimental data regarding the synthesis and structural characterization by: 1H-NMR, 13C-NMR, IR spectral analysis, melting point and thin layer chromatography of the candesartan key intermediate: methyl 2-[(tert-butoxycarbonyl)amino]-3-nitrobenzoate. In addition, a computational study of predicted molecular parameters, vibrational wavenumbers, frontier molecular orbitals energy diagram, molecular electrostatic potential map and other electronic distributions maps using restricted hybrid HF-DFT SCF calculation has been performed for obtaining the most stable conformer. For the most Stable conformer has been made a series of DFT calculations using the B3LYP levels using the 6-31G * basis set.
In vitro Response of Human Osteoblasts Cultured on Strontium Substituted Hydroxyapatites
Authors: Rapuntean Sorin , Frangopol Petre T. , Hodisan Ioana , Tomoaia Gheorghe , Oltean-Dan Daniel , Mocanu Aurora , Prejmerean Cristina , Soritau Olga , Racz Levente Zsolt , Tomoaia-Cotisel Maria
Published: February 2020

The goal of this study was to analyze the response of osteoblasts cultured on strontium substituted hydroxyapatites (HAP-Sr) of well-defined high crystallinity deposited as thin films on glass plates. Up to now, this aspect has not been carefully investigated in the context of bio-ceramics. In this study, we present the osteoblasts activity on synthesized HAP-Sr for different amounts of strontium substitution for calcium within the hydroxyapatite (Ca10(PO4)6(OH)2, HAP) lattice, namely HAP-5%Sr, HAP-10%Sr, HAP-15%Sr and HAP-59.2%Sr (Sr-HAP, of formula Sr10(PO4)6(OH)2), in comparison with stoichiometric pure HAP, chosen as control. Each bio-ceramic was deposited as thin multilayers self-assembled substrate (scaffold) and chemically bonded to the surface of glass plates. These coatings revealed by AFM and SEM imaging a granular texture formed from bio-ceramic nanoparticles. They possessed a high degree of crystallinity, i.e. 68% to 86%, depending on the Sr amount within the HAP lattice, as judged by XRD. Osteoblasts were cultured up to 21days and displayed enhanced adhesion and proliferation particularly evidenced on relatively high strontium contents (especially 5 and 10 weight %, determined by SEM-EDX), where the alkaline phosphatase activity and type I collagen were strongly evidenced. These bio-ceramics showed a high in vitro biocompatibility stimulating the activity of osteoblasts in the process of bone formation. These nano biomaterials can have applications in orthopedic and dental surgery improving the osteointegration as coatings of bone implants as well as for bone repair and regeneration.
Effect of Ceratonia siliqua Methanolic Extract and 6-hydroxydopamine on Memory Impairment and Oxidative Stress in Zebrafish (Danio rerio) Model
Authors: Dumitru Gabriela , Abidar Sara , Nhiri Mohamad , Hritcu Lucian , Boiangiu Razvan Stefan , Sandu Ion , Todirascu-Ciornea Elena
Published: February 2020

The richness in soluble sugars and polyphenols, especially condensed tannins, vitamins, minerals and other phytochemicals, recommends Ceratonia siliqua L. species for use in therapeutic practice, especially in terms of neurotoxicity - a major public health problem. Neurodegenerative disorders arise from various metabolic dysfunctions or from the administration of chemicals such as 6-hydroxydopamine (6-OHDA), a catecholaminergic neurotoxin that induces oxidative stress and, implicitly, cellular apoptosis. The results obtained after the administration of the methanolic extract of carob in Danio rerio highlights a phenomenon of prevention of short-term memory deficits, as well as a significant antioxidant activity.
A Pilot Study for Testing the Efficiency of a Novel Integrated On-line Platform Using Tumoral Biomarkers Evaluation in Colorectal Cancer
Authors: Isaic Alexandru , Cheveresan Adelina , Dobrescu Amadeus , Tarta Cristi , Stoica Laurian , Duta Ciprian , Fulger Lazar
Published: February 2020

Colorectal cancer (CRC) is one of the leading causes of death among cancer patients in Romania. It is known as a heterogeneous disease, characterized by diversion in multiple molecular pathways during its evolutionary process. This research was focusing on the two biomarkers that are related to CRC, specifically Carcinoembryonic Antigen (CEA) and Carbohydrate Antigen 19-9 (CA 19-9). Being an integral part of a wider study, we explored the use of a novel platform to support the strictly controlled process of data collection on colorectal cancer cases. We collected data about the patients regarding baseline clinical parameters, medical history, important comorbidities, intraoperative findings and adverse events, tumor characteristics, histological findings and main laboratory data (blood cell count, serum protein electrophoresis, liver and kidney function tests, serum levels of CEA and CA19-9). The results suggest that these two biomarkers are useful preoperative indicators of local recurrences and prognosis in colorectal cancer. Increased levels of both tumor markers are correlated with an advanced stage of the disease and a poor prognosis. Finally, this study aims to provide the necessary infrastructure, geared towards efficient, safe and comprehensive clinical evaluation of CRC cases.
Drug Hypersensitivity Reduction Using Encapsulation Method with Chitosan-Cetirizine Derivatives
Authors: Dragostin Ionut , Dragostin Oana Maria , Dragan Maria , Stan Catalina Daniela , Zamfir Carmen Lacramioara
Published: February 2020

The ideal material for encapsulation should have good rheological properties, good dispersing capacity of active substances, lack of chemical reactivity with encapsulated substances, the ability to complete release the active compounds, under different conditions, solubility in non-toxic solvents. The degree of novelty of this work consists in applying a preclinical solution for reducing the risk of allergic reaction manifestation, after the treatment with therapeutic agents with allergic potential, as it is the case of beta-lactam antibiotics or non-steroidal anti-inflammatory drugs. The originality of this research it is based on obtaining new chitosan derivatives, starting from cetirizine which is well known for its antihistaminic properties, followed by the use of these new derivatives for encapsulation of drugs with allergic potential. The obtained particles will lead to the reducing of the drug hypersensitivity, keeping, at the same time, the followed therapeutic effect.
Influences of Vitamin A and E Supplementation on Haematological, Functional and Oxidative Balance Parameters on Handball Players
Authors: Badau Dana , Badau Adela , Paraschiv Florin , Rogozea Liliana Marcela , Hussein Hamida Al , Brinzaniuc Klara
Published: February 2020

The objective of this study was to evaluate the antioxidant effect of the vitamins A and E before and after intensive aerobic exercise effort and to provide pros and cons arguments on the use of both vitamins during the physical training. The research highlighted important adaptive changes of the functional parameters: PMA, VCEE, VO2max, of the oxidative balance: LPx, MDA, as well as the haematological parameters: HGB, CRT, LYM, LAK.
Correlation Criteria Between Extramural Invasion of Blood Vessels and Immunohistochemical Markers in the Processes of Neovasculogenesis
Authors: Hinganu Delia , Hinganu Marius Valeriu , Bulimar Virgil , Andronic Dan
Published: February 2020

Establishing the treatment strategy for patients diagnosed with colorectal cancer is based on their imaging and immunohistochemical evaluation. Depending on the stage of tumor invasion and its aggressiveness, is individualized, surgical, oncological, or both treatment. The most important factor for establishing these protocols is the impact of the tumor on intra- and perilesional colorectal vasculature and implicitly on the process of vascular neoformation in the paraneoplastic syndrome. In this regard, we studied a group of patients, colonoscopically diagnosed with colorectal cancer. The study method was to compare the expression of CD34 tumor marker with EMVI (extramural vascular invasion) staging criteria. The results of our study were compared with the intraoperative aspects. In all cases studied tumor extension reached to at least intestinal muscle layer. We conclude that expression of the CD34 marker has always been increased in correlation with EMVI 3-4 stages. In 2 patients with EMVI score 2, the immunohistochemical marker expression was 6% above the maximum threshold. The expression of vascular endothelial proliferation marker is closely correlated with the EMVI score - these values increase proportionally. The use of both criteria for establishing neovasculogenesis, significantly increases the predictability of postoperative progression and long-term survival.
Salvia Officinalis Essential Oil Loaded Gelatin Hydrogel as Potential Antibacterial Wound Dressing Materials
Authors: Gherman Timea , Popescu Violeta , Carpa Rahela , Gavril Georgiana Luminita , Rapa Maria , Oprescu Elena Emilia
Published: February 2020

Salvia officinalis essential oil loaded gelatin hydrogels with improved antibacterial activity and enhanced stability was prepared by microwave-assisted polymerization method. FT-IR spectra indicated no chemical interaction between the hydrogel matrix and the essential oil functional groups. According to the swelling studies, enhanced stability in all pH media was obtained. Studying two kinetic models: Fickian transport and Schott second order kinetic model, it was demonstrated that the swelling process of the prepared hydrogels occurs after a second order kinetics. Antibacterial activity, investigated by the agar diffusion method, regarding S. aureus and E. coli is comparable to that of silver nanoparticles and twice more efficient compared to cinnamon essential oil.
Immunohistochemical Expression of CD8, CDX2, P53, D2-40 and KI 67 in Colorectal Adenocarcinoma, Conventional and Malignant Colo-rectal Polyps
Authors: Mesina Cristian , Stoean Liviu Catalin , Stoean Ruxandra , Sandita Victor Adrian , Gruia Corina Lavinia , Foarfa Maria Camelia , Rotaru Luciana Teodora , Ciobanu Alina Elena , Mesina Mihai , Calborean Veronica , Gheorman Victor , Ciobanu Daniela
Published: February 2020

82 patients, who had been diagnosed with colo-rectal adenocarcinoma in our department between 2007 and 2014, were included in our study. Additionally, 31 patients with colo-rectal polyps (20 conventional adenomatous polyps and 11 malignant colo-rectal polyps) were also included in this study. The patients with colo-rectal adenocarcinoma were reevaluated in terms of gender, age, topography of recto-colic tumor, TNM stadialization, vasculo-lymphatic invasion, lymph nodes metastases and distant metastases. The study of CD8, Ki67, p53, CDX2 and D2-40 immunoexpression in colo-rectal cancer patients revealed: an elevated positivity index in patients with pT2 and pT3 stages and in patients with lymph node metastases in case of the CD8; an elevated positivity index in patients with pT2, pT3 and pT4 stage in case of Ki67; an elevated positivity index in patients with pT4 stage in in case of the p53; an increased positivity index in the pT2, pT3 stage, the absence of vasculo-lymphatic invasion, the absence of lymph node and distant metastases in case of the CDX2 and an increased positivity index in patients with the pT2, pT3 stage, the abscence of lymph node and distant metastases in case of the D2-40 Tumoral staging (pT2, pT3 and pT4) has been shown to be correlated with immunoexpression of the following markers: CD8, CDX2 and p53.
Geochemical and Spatial Distribution of Heavy Metals in Forest Soils Adjacent to the Tinovul Mare Poiana Stampei Peat Bog
Authors: Cazacu Bogdan Constantin , Buzgar Nicolae , Iancu Ovidiu Gabriel
Published: February 2020

Tinovul Mare Poiana Stampei is situated in the northern group of the Oriental Carpathians within the Dornelor Depression, one of the richest peat areas in Romania. In this study we have performed analyses of certain heavy metals (Cr, Co, Ni, Cu, Zn, Pb, As and Cd) found in the forest soils adjacent to the Tinovul Mare Poiana Stampei peat bog. The analyses performed through X-ray fluorescence spectrometry indicated the following variation limits (mg/kg): Cr: 20-66; Co: 8-29; Ni: 16-78; Cu: 16-42; Zn: 36-199; Pb: 21-229; As: 5.5-36 and Cd: 0.01-1.4. The outcomes of the present study indicate high concentrations for certain (Ni, Pb and As). These concentrations exceed the alert threshold established by the Romanian Law, according to Order no. 756 of November, 3rd 1997.
Analysis of the Amount of Polyphenols, Flavonoids and Assessment of the Antioxidant Capacity of Frozen Fruits
Authors: Pallag Annamaria , Jurca Tunde , Sirbu Vasile , Honiges Ana , Jurca Claudia
Published: February 2020

The data from literature shows that frozen vegetal products preserve their nutritional qualities for a long time. Fruits have a high content of substances with antioxidant capacity in the body. There are many recent studies demonstrating the importance of antioxidant substances in neutralizing free radicals in the human body. In this study there were analysed phenolic compounds and flavonoids in eight different species of fruits, belonging to three families. The antioxidant capacity of the extracts was evaluated by: CUPRAC assay, ABTS method and FRAP method. Our results show that the studied fruits represent rich sources of compounds with antioxidant capacity.
Iodinated Contrast Media in Pediatric Cardiac Angiography Nephrotoxic risk evaluation
Authors: Cinteza Eliza , Balgradean Mihaela , Filip Cristina , Duica Gabriela , Nicolae Georgiana , Nicolescu Alin , Mahmoud Hyam
Published: February 2020

Contrast agents are among the most frequently used drugs in medical practice. The aim of our study was to evaluate the incidence of complications associated with the use of different contrast agents in an angiography lab dedicated to pediatric patients with congenital heart disease. Between June 2015 and December 2017, 166 patients with congenital heart disease were diagnosed and/or treated in the angiography lab. Of these patients, 38 were excluded because they did not require contrast substance administration. As interventional procedures we performed pulmonary valvular dilatation in 17 cases (10.2%), stent implantation in coarctation of the aorta in 9 cases (5.4%), PDA closure in 62 cases (37.3%), atrial septal defect (ASD) closure in 36 patients (including patients without contrast agent�s administration) (21.7%). In the group of 129 patients who received contrast agents, the median age was 5.8 years (range 0.1-19 years) and median weight 22.8 kg (range 2.8-72 kg). Average consumption of contrast media per procedure was 79 ml/procedure (range 5 - 400 mL) and 4.3 mL/kg (range 0.4 - 22.7). We used iomeprol (24%), iohexol (8.5%), iopromide (67.4%). No contrast related complications are reported in this group. In conclusion, the contrast agents we used seem to be safe and are not associated with renal complications.
High Temperature Ionic Liquids Electrosynthesis of Mo2C Protective Coatings on Diamond, Boron Nitride, Silicon and Boron Carbide
Authors: Malyshev Victor , Gab Angelina , Shakhnin Dimitri , Donath Cristina , Neacsu Elena Ionela , Popescu Ana Maria , Constantin Virgil
Published: February 2020

On the basis of the thermodynamic analysis of the reactions of dielectrics and semiconductors with ionic melts, the systems suitable for an electrochemical synthesis from high temperature ionic liquids of films consisting in molybdenum carbide on the surfaces of these materials were selected. Then, the study of their electrochemical behavior was performed.
Risk Behaviours for Traffic Accidents in 18-20 Years Young People Travelling with Drunk Driver of Timis County, Romania
Authors: Pantea Cristina , Horhat Razvan , Putnoky Salomeia , Suciu Oana , Sas Ioana Tuta , Bacean Codruta , Bagiu Radu , Mladinescu Corneluta Fira , Vlaicu Brigitha
Published: February 2020

The present research aimed to assess some predictors for experiencing traveling in a car with a driver who has consumed alcohol, in a group of young people, aged between 18 and 20 years, residents of Timis County, Romania. The study group of 1606 young subjects, 18-20 years of age, 51.4% pupils and 48.6% students, with girls being significantly better represented, was applied a transversal population study. Percents of 29.8% of boys and 28.4% of girls got 1-3 times in a car with a drunk driver, and 10.1% of boys and 6.5% of girls traveled in such circumstances more than 4 times. Boys tend to accept the risks of traveling in a car with a drunk driver significantly more frequently than girls. We identified some predictors for traveling with a driver who has consumed alcohol, such as the binge drinking model and the model of mixed alcohol and drugs consumption, the practice of alcohol consumption associated with vehicle driving by the father, as well as by friends.
Pretreatments Testing of High Biodiversity Inocula with Simultaneous Biohydrogen Production and Wastewater Treatment
Authors: Gherman Vasile Daniel , Molnar Paul , Motoc Marilena , Negrea Adina
Published: February 2020

Hydrogen represents a renewable energy resource and it is an ideal alternative to fossil fuels since it does not contribute to the greenhouse effect. In the present study it has been proposed to develop an experimental model to test and compare fermentative capacity of waste water with biohydrogen production for two high biodiversity heterotrophic microbial inocula. All the pretreatment methods tested yielded good results, but heat and acid pretreatment had the best results. These observations open the way for the development and application of new technologies using microbial consortia specially developed to serve the dual role of biological waste water treatment and the production of a renewable energy in the form of biohydrogen.
Evaluation of Surface Modification of PA 2200 Parts Made by Selective Laser Sintering Process
Authors: Borzan Cristina Stefana Miron , Moldovan Marioara , Bocanet Vlad
Published: February 2020

Cellular adhesion on surface structures from PA 2200 made through Selective Laser Sintering (SLS) process can be improved by modifying the parts surface. In this paper, different methods for surface modification are presented. The PA 2200 samples were immersed in six different solvents. SEM images and surface roughness tests were performed in order to evaluate the surface modification both for the control group and for the tested groups. The obtained results clearly show that there is a significant difference between the roughness of the control (untreated) surface and each of the treated surfaces. Modifying by immersion, of the PA 2200 structures produced by Selective Laser Sintering process, brings original approaches concerning the use of those procedures in the increase of the surfaces quality.
Garlic and Chlorella Biomodulate Lead Toxicity on Manganese Homeostasis in Carassius gibelio Bloch
Authors: Nicula Marioara , Pacala Nicolae , Stef Lavinia , Pet Ioan , Dronca Dorel , Ahmadi Mirela , Gherbon Adriana
Published: February 2020

Environmental pollution negatively affects the aquatic ecosystems. Heavy metals are considered dangerous toxic elements for aquatic biota, as metallic elements or as salts which exhibit great stability. Among these, lead is toxic for all aquatic biotic components. Our research investigated the manganese distribution in tissue of Prussian carp fishes after lead intoxication, highlighted also the lead detoxifying potential of active principles from garlic and chlorella (powder). For our experiment 120 Prussian carp fingerlings, weighing 22-25g each, for 21 consecutive days, were divided according to the following treatments: C group (without treatment), E1 group (75ppm Pb in water as Pb(NO3)2 x �H2O), E2 group (75ppm Pb in water+2% freeze-dry garlic in feed), E3 group (75ppm Pb in water+2% freeze-dry chlorella in feed). At the end of the experimental period, tissue samples (gills, muscle myotomers�epaxial, heart, skin and scales, intestine, liver, brain, gonads, kidney) were collected after euthanasia with clove oil. Manganese concentration was analytical performed using AAS (atomic absorption spectrometry). Using fish as bioindicators of lead experimental contamination allowed us to obtain valuable informations about its ability to substitut/remove bioactiv minerals from animal tisssues. In the same time, we could emphasize the efficiency of natural antioxidants or chelators of edible plants (such as garlic) or algae (such as chlorella), to alleviat the lead impact on homeostasis of trace elements from tissues.
Influence of the Bone Cements Processing on the Mechanical Properties in Cranioplasty
Authors: Rivis Mircea , Pricop Marius , Talpos S. , Ciocoiu Robert , Antoniac Iulian , Gheorghita Daniela , Trante Octavian , Moldovan Horatiu , Grigorescu Gabriel , Seceleanu Vicentiu , Mohan Aurel , Niculescu Serban Talpos
Published: February 2020

The aim of this study is to observe the time of mixing influence on the properties of commercially available PMMA bone cement, widely used in cranioplasty. The studied bone cement is provided in the form of a solid powder (the copolymer) and a liquid monomer. The increase of the mixing phase duration and the use of two mixing methods (manual and mechanical) effect on surface and mechanical characteristics were studied. The samples were prepared as if in the operation room. Surface characteristics were studied by means of contact angle measurements, morphology by scanning electron microscopy (SEM) and mechanical characteristics determined by flexural tests in a three point bending configuration. The conclusion of this study is that by using a mechanical mixing method and increasing mixing time higher flexural strength can be achieved by reducing pore content within bone cement.
Association Between Chronic Obstructive Pulmonary Disease and Sleep Apnea - Overlap Syndrome- Experience of Pulmonology Clinic Tg. Mures, Romania
Authors: Jimborean Gabriela , Szasz Simona , Szathmary Mioara , Csipor Alpar , Arghir Oana Cristina , Nemes Roxana Maria , Postolache Paraschiva , Ianos Edith Simona
Published: February 2020

Association between chronic obstructive pulmonary disease (COPD) and sleep apnea SA (overlap syndrome - OS) includes serious clinical manifestations and high mortality due to early respiratory failure, cardiovascular and metabolic complications from both diseases. 90 COPD patients (85.5% males) were strongly suspected to have concomitant SA after clinical examination and sleep questionnaires. We performed a cardio-ventilatory poligraphy during sleep. 82 patients (91.1%) from our OS group had obstructive sleep apnea (OSA), 8 patients (8.9%) mixed apnea and 20% had also OHS. 17 (18.8%) of OS were overweight and 66 (73.3%) obese. A third of them were in a very active group of age: 49 patients (54.4%) under 60 year-old and 11 patients (12.2%) between 61-65 year/old. We noted severe complication/comorbidities in our OS group: 63.3% hypertension, 43.3% core pulmonale, 31.1% arrhythmia, 32.2% cardiac failure, 38.8% dyslipidemia, 31.1% diabetes. The second night investigation permitted titration for the targeted pressures for CPAP therapy (Continuous Positive Airways Pressure). Treatment of OS patients had an interdisciplinary approach: CPAP in OSA, BPAP (Bi-level Positive Airways Pressure) in OHS, inhaled bronchodilators, treatment of cardiovascular comorbities, pulmonary rehabilitation, weight loss, tobacco/alcohol cessation counseling, and oxygen therapy in remaining hypoxemic patients. 51.2% of patients had not accessibility for long time CPAP (lack of coverage by the public health system). OS included clinical aspects of severity due to both COPD and OSA. Clinical investigation, sleep questionnaires, assessment of the diurnal somnolence and sleep cardiorespiratory poligraphy are recommended in all COPD patients. Obesity was the main risk factor for OSA in COPD patients.
Metabolic and Weight Changes After Bariatric Surgery in a Rat Model of Induced Type 2 Diabetes Mellitus and Obesity
Authors: Dobrescu Amadeus , Stoica Laurian , Mituletu Mihai , Isaic Alexandru , Duta Ciprian , Verdes Gabriel , Tarta Cristi , Heghes Alina , Fulger Lazar
Published: February 2020

Sleeve gastrectomy (SG) and gastric plication (GP) are surgical procedures providing weight loss through several mechanisms incompletely understood. Rat models allow the possibility of tight regulation of experimental conditions, making them the ideal candidates for animal models in bariatric surgery. The aim of this study was to compare the weight and metabolic changes between sleeve gastrectomy and gastric plication in a rat model with type 2 induced diabetes mellitus and obesity. Fifteen male Wistar rats were fed with DIO food (Bio Serv �F3282 - Mouse Diet, High Fat, Fat Calories -60%), after 36 weeks were allocated to the study three arms-SG, GP and sham operation (SO). Four weeks after the surgery the rats were weighted again. Blood tests were performed before surgery and four weeks after surgery searching for blood glucose, total cholesterol, HDL, triglycerides, and LDL. At the onset of the study, the rats were 9 weeks old and had an average body mass of 231.6g � 31.58. After 36 weeks of DIO, one day preoperatively body weight was 774.93g � 95.02. The preoperatively average of body weight in the SG group was 777.4g �104.66, 775.4 g �104.6 in the GP group, respectively 772g �79 in the SO group. 4 weeks after surgery the mean body weight in the SG group was 648.8g �99.09, in the GP group was 695.6g �99.09, respectively 825.4g �79.87 in the SO fed ad libitum group. There was a significant decrease of mean fasting glucose levels at 4 weeks postoperative in the SG group compared to the SO group (87.4mg/dL�8.73 versus 103.6 mg/dlL�4.66, p= 0.01). The same trend of mean fasting glucose was registered in the GP group versus the SO group (92.8 � 5.67 mg/dl vs 103.6 mg/dl � 4.66, p=0.01). Our study provides evidence of the positive effects of bariatric surgery for treating patients with morbid obesity associated with diabetes mellitus and dyslipidemia and the use of rats to study the mechanisms of weight reduction and metabolic changes in bariatric surgery.
A Study of Some Mechanical and Chemical Properties of Composite Materials Made Up of UPR and ISO/NPG Granules of the Type of Buzi/Perdido Cromate
Authors: Ciuca Ion , Stanescu Marius Marinel , Bolcu Dumitru , Deaconu (Stanescu) Luminita Florentina
Published: February 2020

In this work we have measured the mechanical properties for unsaturated polyester resin (UPR) and unsaturated polyester resin mixed with isophthalic resin granules NPG (Neopentyl Glycol) of the type of Buzi/Perdido Chromate. All sets of samples underwent compression testing. We obtained the characteristic curve, the admissible resistance to compression, and the modulus of elasticity for each sample. Moreover, on the basis of FTIR analysis we measured the type of bonds (C-O, C=O, C-C and various bonds in the functional groups) in each polymer.
Research on CO Pollution in Urban Areas
Authors: Tatar Adina Milena
Published: February 2020

The environment is an essential element to human existence and being the interference result of natural elements, soil-air-water-climate-biosphere, with elements created by human activity. The purpose of this study has been to monitor carbon monoxide (CO) immissions in the municipality of Targu-Jiu, and the towns of Rovinari and Turceni during 2017 and to interpret the generated data by reference to the limit value for the protection of human health (L.V.), according to Law no. 104/2011. From the analysis of the data generated during the monitoring it has been concluded that for the urban areas that are the subject of the present study, the CO pollutant is not a danger to the population and the environment.
Studies on the Behavior Some Paints in Electro-insulating Fluid Based on Vegetable Esters
Authors: Lingvay Iosif , Bors Adriana Mariana , Ungureanu Livia Carmen , Stanoi Valerica , Rus Traian
Published: February 2020

For the purpose of using three different types of painting materials for the inner protection of the transformer vats, their behavior was studied under actual conditions of operation in the transformer (thermal stress in electro-insulating fluid based on the natural ester in contact with copper for electro-technical use and electro-insulating paper). By comparing determination of the content in furans products (HPLC technique) and gases formed (by gas-chromatography) in the electro-insulating fluid (natural ester with high oleic content) thermally aged at 130 �C to 1000 hours in closed glass vessels, it have been found that the presence the investigated painting materials lead to a change in the mechanism and kinetics of the thermo-oxidation processes. These changes are supported by oxygen dissolved in oil, what leads to decrease both to gases formation CO2, CO, H2, CH4, C2H4 and C2H6) and furans products (5-HMF, 2-FOL, 2 -FAL and 2-ACF). The painting materials investigated during the heat treatment applied did not suffer any remarkable structural changes affecting their functionality in the electro-insulating fluid based on vegetable esters.
Effect of Sewage Sludge Amended Soil on Maize Crop. II. Influence on metal accumulation
Authors: Ilie Leonard , Mihalache Mircea , Scaeteanu Gina Vasile , Madjar Roxana Maria , Popovici Daniela Roxana
Published: February 2020

The aim of the present study was to evaluate the influence of sewage sludge application on metal accumulation (Cu, Mn, Zn, Cd, Ni, Pb) at maize (Zea Mays L.). It was applied different doses varying from 0-600 kg/ha of sewage sludge associated with mineral fertilization. The results indicated that copper and manganese contents increase with fertilization and rates equivalent to 200 kg N/ha resulted in statistically significant copper content in the maize leaves. The highest accumulation of manganese in the leaves was recorded at the sludge rate equivalent to 300 kg N/ha, meanwhile in the maize kernels after the increasing sludge rate, the manganese content recorded a decreasing tendency. The zinc content in the maize leaves significantly increased with the increasing rate of sludge, starting with a rate equivalent to 200 kg N/ha, sewage sludge application being a promoter of zinc accumulation but without becoming excessive. As concerns the zinc content in the maize kernels, sewage sludge rates higher than 300 kg N/ha resulted in statistically assured increases. The cadmium level in the maize leaves increased directly proportional with the rate applied and the values were statistically assured at rates higher than 200 kg N/ha. The cadmium content in the maize kernels was not affected by the treatments applied, as the very low values obtained were below the detection limit of the analytical method. The lead content in the maize leaves increased under the influence of sludge fertilization, but the accumulation was generally low, all values in leaves being under 10 mg/kg and in kernels under 1 mg/kg Pb.
Biochemical Effects of Intraliposomal Angiotensins on Isolated Vascular Smooth Muscle Cells
Authors: Tatarciuc Diana , Vasincu Decebal , Stoleriu Gabriela , Iancu Roxana Irina , Costuleanu Marcel
Published: February 2020

The intracellular renin-angiotensin effectors (peptides, enzymes, receptors) and their effects are intriguing for a lot of systems. That�s why we aimed the effects of intracellularly-administered angiotensins (angiotensin II, angiotensin III, angiotensin IV, angiotensin fragment 1-7), angiotensinogen, CGP-42112A, apelin, and angiotensin receptors blockers (losartan, PD123319), by the means of liposomes, on apoptosis of cultured isolated rat aortic vascular smooth muscle cells. We evidenced that CGP-42112A (a potent AT2 angiotensin II receptor agonist), administered intracellularly, induced the apoptosis of the cultured isolated vascular smooth muscle cells in a much higher proportion than other agonists and antagonists of angiotensin system: CGP-42112A ] angiotensin II] angiotensin III@ angiotensinogen. Moreover, losartan (an AT1 angiotensin II receptor antagonist), administered intracellularly, induced an important degree of apoptosis of cultured isolated vascular smooth muscle cells. Losartan, administered as concomitant treatment for other angiotensin peptides and CGP-42112A, did not significantly modified the apoptotic effects of these peptides. On the other hand, PD123319 (an AT2 angiotensin II receptor antagonist) was able to significantly reduce the losartan effects when administered as co-treatment for 24 h. The same effects were obtained when LY294002, a PI3K/Akt signaling inhibitor, was administered as a co-treatment. We can conclude an involvement of an AT2 angiotensin II receptor and PI3K/Akt signaling in these apoptotic effects induced by some angiotensin peptides and losartan on cultured isolated rat aortic vascular smooth muscle cells.
The Sintering Behaviour and Mechanical Properties of Hydroxyapatite - Based Composites for Bone Tissue Regeneration
Authors: Tecu Camelia , Antoniac Aurora , Goller Gultekin , Gok Mustafa Guven , Manole Marius , Mohan Aurel , Moldovan Horatiu , Earar Kamel
Published: February 2020

Bone reconstruction is a complex process which involves an osteoconductive matrix, osteoinductive signaling, osteogenic cells, vascularization and mechanical stability. Lately, to improve the healing of the bone defects and to accelerate the bone fusion and bone augmentation, bioceramic composite materials have been used as bone substitutes in the field of orthopedics and dentistry, as well as in cosmetic surgery. Of all types of bioceramics, the most used is hydroxyapatite, because of its similar properties to those of the human bone and better mechanical properties compared to b-tricalcium phosphate [1]. Currently, the most used raw materials sources for obtaining the hydroxyapatite are: bovine bone, seashells, corals, oyster shell, eggshells and human teeth. There are two common ways to obtain hydroxyapatite: synthetically and naturally. Generally, for the improvement of the mechanical properties and the structural one, hydroxyapatite is subjected to the sintering process. Considering the disadvantages of hydroxyapatite such as poor biodegradation rate, b-TCP has been developed, which has some disadvantages too, such as brittleness.
For this reason, the aim of this study is to look into the effect of adding magnesium oxide on the sintering behavior, the structure and the mechanical properties of the hydroxyapatite-tricalcium phosphate composites.
Study on Critical Micelle Concentration Influence in Green Synthesis of Silver Nanoparticles Assisted by Sapindus mukorossi Aqueous Extract
Authors: Olteanu Radu Lucian , Nicolescu Cristina Mihaela , Bumbac Marius , Dulama Ioana Daniela , Ion Rodica Mariana , Suica Bunghez Ioana Raluca
Published: February 2020

Silver nanoparticles synthesis mediated by plant extracts involves the colloidal stability of generated metallic particles in solutions. Formation of micelles in solutions influences the nanoparticle synthesis process and consequently, the surface plasmonic response (SPR) of the formed silver nanoparticles. Critical micelle concentration (CMC) of Sapindus mukorossi aqueous extract has been determined with ultraviolet-visible spectroscopy, two methods were used: dye micellization method, and correlation of silver nanoparticles SPR with the variation of surfactant concentration. Results obtained for CMC determination by the two methods were highly reproducible and in good correlation indicating that micelles formed by saponins present in the Sapindus mukorossi aqueous extract are useful as effective structure-driving agents to synthesize colloidal metallic nanoparticles, offering them proper growth conditions. Silver nanoparticles synthesis mixtures were studied, with Sapindus mukorossi aqueous extract and silver nitrates as precursors, in acidic and basic media, at room temperature to establish correlations between saponins structure modifications and surface plasmonic response of metallic nanoparticles. Light absorption spectrometric techniques (ultraviolet-visible and infrared), X-ray diffraction and scanning electron microscopy were used to characterize the synthesis process.
The Use of Methylene Blue Staining Test in Determining the CSF Leaks Location of the Anterior Skull Base
Authors: Hainarosie Razvan , Ghindea Teodora , Ionita Irina Gabriela , Hainarosie Mura , Stefanescu Cristian Dragos , Zainea Viorel , Rusescu Andreea , Pituru Silviu , Pietrosanu Catalina
Published: February 2020

Cerebrospinal fluid rhinorrhea represents drainage of cerebrospinal fluid into the nasal cavity. The first steps in diagnosing CSF rhinorrhea are a thorough history and physical examination of the patient. Other diagnostic procedures are the double ring sign, glucose content of the nasal fluid, Beta-trace protein test or beta 2-transferrin. To establish the exact location of the defect imagistic examinations are necessary. However, the gold standard CSF leakage diagnostic method is an intrathecal injection of fluorescein with the endoscopic identification of the defect. In this paper we analyze a staining test, using Methylene Blue solution, to identify the CSF leak�s location.
Hydrodesulphurization of Thiophenes over CoMoRe/ ZSM 5gama-Al2O3 Catalyst
Authors: Doukeh Rami , Bombos Mihaela , Moldovan Marioara , Bolocan Ion
Published: February 2020

The hydrodesulphurization (HDS) of thiophenes was performed on metallic catalyst CoMoRe/ZSM5-gAl2O3. Experiments were carried out on a fixed bed catalytic reactor at 175-300�C, 30-60 atm, thiophene volume hourly space velocities of 1h-1- 4h-1 and molar ratio hydrogen/thiophene of 60/1. Texture features like specific surface area, the pore volume and the average pore diameter decrease after catalyst sulfurization. The thiophenes conversion on CoMoRe/gAl2O3-Zn-HZSM 5 catalyst differs with the nature of the studied thiophenes.
Biochemical Features of an Acute Viral Hepatitis A Outbreak
Authors: Cambrea Simona Claudia , Arghir Oana Cristina , Rascu Agripina , Petcu Cristian Lucian
Published: February 2020

In developing countries, outbreaks of acute hepatitis A virus (HAV) infection have a cyclic recurrence and almost 90% of children go through disease by the age of 10. Although the evolution of HAV is rarely severe, it can cause significant economic and social losses. In order to analyze clinical and biochemical characteristics of acute HAV reported diseases in Constanta county, South Eastern Romania, during the last pediatric outbreak, all cases of hospitalized children, less than 13 years old (n=578), mostly boys with residence in urban cities, were included. Cases were divided into 295 isolated cases, mean aged 6.939 years, and 283, mean aged 6.587 years, diagnosed in different foci of the outbreak. Clinical and biochemical features of an acute HAV outbreak in the foci children��s collectivities and families consisted in mild form of disease with frequent hepatomegaly, lack of jaundice and lower levels of aminotransferases and bilirubin.
3D Reconstruction Role in Surgical Treatment of Sinonasal Tumours
Authors: Palade Dragos Octavian , Cobzeanu Bogdan Mihail , Zaharia Petronela , Dabija Marius
Published: February 2020

Three-dimensional printing has numerous applications and has gained much interest in the medical world. The constantly improving quality of 3D-printing applications has contributed to their increased use on patients. Nowadays, 3D printing is very well integrated in the surgical practice and research. Also, the field of head and neck reconstructive surgery is constantly evolving because of the three-dimensional printing, a technology which can be widely used in a variety of situations such as reconstruction of tissue defects, surgical planning, medical modeling and prosthesis. By using 3D printing into tissue engineering and materials, it may be possible for otolaryngologists to implant 3D printed functional grafts into patients and will also provide a rapid production of personalized patient-specific devices. Advances in 3D printed implants and future tissue-engineered constructs will bring great progress to the field of otorhinolaryngology.
The Assessment of Chitosan Solutions Effects on Bacterial Strains
Authors: Filimon Marioara Nicoleta , Popescu Roxana , Sinitean Adrian , Maniu Paula , Dumitrescu Gabi , Verdes Doina , Vlad Cristian Sebastian
Published: February 2020

The interest in the antimicrobial actions of chitosan is due to its multiple properties and effects. The aim of the study was to assess the potential antibacterial effects of chitosan applied on 7 bacterial strains: Escherichia coli, Streptococcus pyogenes, Pseudomonas aeruginosa, Enterococcus faecalis, Clostridium perfringens, Legionella pneumophila and Staphylococcus aureus. Six different concentrations of chitosan were dissolved in 1% acetic acid, following two working protocols (Kirby-Bauer method and testing for bacterial cell viability). The sensitivity of tested bacterial strains following the effect of exposure to chitosan decreased as follows: E. coli] L. pneumophila] S. aureus] S. pyogenes] C. perfringens] P. aeruginosa] E. faecalis. The inhibition rates for the bacterial strains E. faecalis, S. pyogenes and S. aureus highlighted again the strong antibacterial properties of this product. Conclude that the chitosan presents a different antibacterial effect against several bacterial strains of interest directly with the employed concentrations.