bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification and Characterization of the Haloperoxidase VPO-RR from Rhodoplanes roseus by Genome Mining and Structure-Based Catalytic Site Mapping
Authors: Porta Nicola , Fejzagić Alexander Veljko , Dumschott Kathryn , Paschold Beatrix , Usadel Björn , Pietruszka Jörg , Classen Thomas , Gohlke Holger
Published: October 2022

Halogenating enzymes have evolved in considerable mechanistic diversity. The apparent need for secondary metabolism coincides with the current need to introduce halogens in synthetic products. The potential of halogenating enzymes and, especially, vanadate-dependent haloperoxidases has been insufficiently exploited for synthetic purposes. In this work, we identified potential halogenase sequences by screening algal, fungal, and protobacterial sequence databases, structural modeling of putative halogenases, and mapping and comparing active sites. In a final step, individual haloperoxidases were expressed and kinetically characterized. A vanadate-dependent haloperoxidase from Rhodoplanes roseus was heterologously expressible by E. coli and could be purified to homogeneity. The kinetic data revealed a higher turnover number than the known VClPO-CI and no inhibitory effect from bromide, rendering this enzyme a promising biocatalyst. Other predicted haloperoxidases were not expressed successfully yet but these enzymes were predicted to be present in a wide taxonomic variety.
Emerging Copper-Based Semiconducting Materials for Photocathodic Applications in Solar Driven Water Splitting
Authors: Kumar Mohit , Meena Bhagatram , Subramanyam Palyam , Suryakala Duvvuri , Subrahmanyam Challapalli
Published: October 2022

Hydrogen production through solar-driven water splitting is a promising approach and an alternative to the conventional steam reforming of natural gas and coal gasification. The growing energy demand and environmental degradation through carbon-emitting fossil fuels urge a transition in the usage of non-renewable to renewable sources of energy. The photocathodes in a photoelectrochemical (PEC) water-splitting cell are essential for the direct evolution of hydrogen. Among the known photocathodes, Cu-based p-type semiconducting materials are the most promising photo-absorber materials owing to their low-cost, low toxicity, natural abundance, suitable bandgaps, and favorable band edges for reduction. Moreover, the chemical stability and the rate of recombination significantly limit the longevity, the PEC performance, and practical applicability of Cu-based photocathodes. To overcome these problems, it is critical to have a thorough understanding of the constraints, improvement strategies, and an assessment of current developments in order to construct and design highly stable and efficient photocathodes. Here, in this review we have summarized the development of Cu-based metal oxide and sulfide photocathodes with the significant operational challenges and strategies that have successfully been employed to enhance the PEC performance. Furthermore, the emphasis is placed on recent reports and future perspectives regarding emerging challenges.
Mesenchymal Stem/Stromal Cells as a Therapeutic Tool in Cell-Based Therapy and Regenerative Medicine: An Introduction Expertise to the Topical Collection
Authors: Merimi Makram , Fahmi Hassan , De Kock Joery , Beguin Charline , Burny Arsène , Moll Guido , Poggi Alessandro , Najar Mehdi
Published: October 2022

We are pleased to present this opening editorial, introducing our topical collection, “The New Era of Mesenchymal Stromal/Stem Cell Functional Application: State of the Art, Therapeutic Challenges and Future Directions” [...]
Stereological Estimation of Myocardial Fat and Its Associations with Obesity, Epicardial, and Visceral Adipose Tissue
Authors: Holm Pernille Heimdal , Hindsø Louise , Olsen Kristine Boisen , Banner Jytte
Published: October 2022

The normal human heart contains epicardial adipose tissue (EAT) and myocardial fat. The associations between obesity, myocardial fat, visceral adipose tissue (VAT), and cardiovascular disease are not fully understood. The objective of this study was to estimate myocardial fat using stereological methods and investigate its relations with obesity, EAT, and VAT. To establish the EAT volume, 115 deceased individuals were included, and postmortem computed tomography was conducted on their eviscerated hearts. Six samples from the left and right ventricles (LV and RV) of the heart were stereologically examined to calculate the percentage of myocardial fat. Kidney and omental fat were weighed at autopsy, and the waist–hip ratio was calculated. Females had a slightly non-significantly (p = 0.054) larger proportion of RV fat (13.2% ± 4.4) compared to that in men (11.5% ± 2.7). We found a significant positive correlation between body mass index (BMI) and LV myocardial fat (p = 0.033). In the RV, this correlation was only at the borderline of significance (p = 0.052). The EAT volume was positively correlated with both RV and LV myocardial fat. We found no association with the waist–hip ratio (WHR) or the omental or kidney fat as measures of VAT. The myocardial fat was normal, most prominent in the RV, and correlated with the EAT and, partly, BMI. We found no association with VAT.
Cytotoxicity and Lipase Inhibition of Essential Oils from Amazon Annonaceae Species
Authors: de Lima Barros André , de Lima Emilly J. S. P. , Faria Jéssica V. , Acho Leonard R. D. , Lima Emerson S. , Bezerra Daniel P. , Soares Elzalina R. , de Lima Bruna R. , Costa Emmanoel V. , Pinheiro Maria Lúcia B. , Bataglion Giovana A. , da Silva Felipe M. A. , Cardozo Nállaret M. Dávila , Gonçalves José F. C. , Koolen Hector H. F.
Published: October 2022

Essential oils from Amazonian species are gaining increasing interest worldwide due to their medicinal and cosmetic applications; however, the relation among the chemical constituents and their biological properties are not well explored. Therefore, the present research aims to obtain an understanding of the bioactivity of chemical compounds in the essential oils of plants from the Annonaceae family (Bocageopsis pleiosperma, Onychopetalum amazonicum, Unonopsis duckei, U. floribunda, U. rufescens, U.stipitata, U. guatterioides, Duguetia flagellaris and Xylopia benthamii). By means of gas chromatography coupled to mass spectrometry, in vitro cytotoxic and anti-lipase assays, principal component analysis and molecular docking, it was possible to establish the main compounds that may be responsible for the cytotoxic effect of O. amazonicum and B. pleiosperma. Moreover, the anti-lipase potential of D. flagellaris was also established, as well as its composition related to the activity. Thus, by the employed strategy, allo-aromadendrene, cryptomerione, δ-cadinene and β-bisabolene were suggested as plausible cytotoxic agents against cancer cell lines, and dehydroaromadendrene, spathulenol and elemol, against lipase. The present study provides significant information on the chemical profile and bioactivity studies of Amazon Annonaceae aromatic plants.
Hypervalence: A Useful Concept or One That Should Be Gracefully Retired?
Authors: Norman Nicholas C. , Pringle Paul G.
Published: October 2022

In this essay the origins of the term hypervalence and its application in p-block element chemistry are considered and it is argued that the term should now be consigned to the graveyard of concepts that no longer afford any discernible value or insight, certainly from an educational perspective. In contrast, the educational merits of the octet rule are also examined where it is concluded that this rule does have significant pedagogical value, albeit mostly within the ambit of introductory level explanations. For a few of the chosen exemplar compounds, a selection of orbital-based analyses, at different levels of sophistication, are also considered, and their values appraised together with a brief survey of some of the more general computational studies which have been employed in relation to this topic.
Indium Oxide Decorated WS2 Microflakes for Selective Ammonia Sensors at Room Temperature
Authors: Guang Qiyilan , Huang Baoyu , Yu Jun , Zhang Jianwei , Li Xiaogan
Published: October 2022

Tungsten sulfide decorated with indium oxide nanoparticles (In2O3/WS2) was studied for a chemiresistive-type NH3 sensor at room temperature. It was found that the responses of the developed In2O3/WS2 heterostructure nanocomposite-based sensors are significantly improved to 3.81 from 1.45 for WS2. The response and recovery time of the heterostructure-based sensor was found to significantly decrease to 88 s/116 s (10 ppm) from 112 s/192 s for the WS2-based one. The sensor also exhibits excellent selectivity and signal reproducibility. In comparison to WS2 decorated with both ZnO and SnO2 in similar ways, the In2O3-decorated WS2 has overall better sensing performance in terms of sensitivity, selectivity and response/recovery speeds for NH3 from 1 ppm to 10 ppm at room temperature. The improved sensing properties of WS2 incorporating In2O3 could be attributed to the joint enhancement mechanisms of the “electronic and catalytic” sensitizations.
The Functional Fe3O4@SiO2@AuNPs SERS Nanomaterials for Rapid Enrichment and Detection of Mercury Ions in Licorice
Authors: Zhu Jieqiang , Wang Baoling , Yang Ping , Li Junmei , Xiao Guyu , Yao Jiangyu , Gong Xingchu , Yan Jizhong , Zhang Hui
Published: October 2022

There has been an increasing demand for rapid and sensitive techniques for the detection of heavy metal ions that are harmful to the human body in traditional Chinese medicine (TCM). However, the complex chemical composition of TCM makes the quantitative detection of heavy metal ions difficult. In this study, the magnetic Fe3O4@SiO2@AuNPs nanoparticles combined with a probe molecule DMcT were used for the specific enrichment and detection of Hg2+ in the complex system of licorice. The core of Fe3O4 was bonded with SiO2 to increase its stability. A layer of AuNPs was deposited to produce a “core–shell” Raman substrate with high surface-enhanced Raman spectroscopy (SERS) activity, which was surface modified by DMcT probe molecules with sulfhydryl groups. In the presence of Hg2+, Hg2+ binds to N on the amino group of DMcT to form N-Hg2+-N complexes, which induces Fe3O4@SiO2@AuNPs-DMcT clustering to enhance SERS signal. The Raman probe molecule DMcT showed an excellent linear relationship (R2 = 0.9709) between the SERS signal at 1416 cm−1 and the Hg2+ concentration (0.5~100 ng/mL). This method achieved a good recovery (89.10~111.00%) for the practical application of detection of Hg2+ in licorice extracts. The results demonstrated that the functional Fe3O4@SiO2@AuNPs-DMcT performed effective enrichment and showed high sensitivity and accurate detection of heavy metal ions from the analytes.
Western Diet-Fed ApoE Knockout Male Mice as an Experimental Model of Non-Alcoholic Steatohepatitis
Authors: Camargo Felipe N. , Matos Sandro L. , Araujo Layanne C. C. , Carvalho Carla R. O. , Amaral Andressa G. , Camporez João Paulo
Published: October 2022

One of the consequences of the Western lifestyle and high-fat diet is non-alcoholic fatty liver disease (NAFLD) and its aggressive form, non-alcoholic steatohepatitis (NASH), which can progress to cirrhosis and hepatocellular carcinoma (HCC) and is rapidly becoming the leading cause of end-stage liver disease or liver transplantation. Currently, rodent NASH models lack significant aspects of the full NASH spectrum, representing a major problem for NASH research. Therefore, this work aimed to characterize a fast rodent model with all characteristic features of NASH. Eight-week-old male ApoE KO mice were fed with Western diet (WD), high fatty diet (HFD) or normal chow (Chow) for 7 weeks. Whole-body fat was increased by ~2 times in WD mice and HFD mice and was associated with increased glucose intolerance, hepatic triglycerides, and plasma ALT and plasma AST compared with Chow mice. WD mice also showed increased galectin-3 expression compared with Chow or HFD mice and increased plasma cholesterol compared with Chow mice. WD and HFD displayed increased hepatic fibrosis and increased F4/80 expression. WD mice also displayed increased levels of plasma MCP-1. Hepatic inflammatory markers were evaluated, and WD mice showed increased levels of TNF-α, MCP-1, IL-6 and IFN-γ. Taken together, these data demonstrated that the ApoE KO mouse fed with WD is a great model for NASH research, once it presents the fundamental parameters of the disease, including hepatic steatosis, fibrosis, inflammation, and metabolic syndrome.
Special Issue: Advanced Electrochromic Materials and Devices
Authors: Zhang Shengliang , Peng Yutao , Zhao Jing , Zhang Xiaogang
Published: October 2022

Electrochromism is the phenomenon where the optical properties (transmittance, absorbance or reflectance) of a material are dynamically and reversibly changed by the application of a small external electric field [...]
Bimetallic Assembled Silver Nanoparticles Impregnated in Aspergillus fumigatus Extract Damage the Bacterial Membrane Surface and Release Cellular Contents
Authors: Saqib Saddam , Faryad Saima , Afridi Muhammad Irfan , Arshad Bushra , Younas Muhammad , Naeem Muhammad , Zaman Wajid , Ullah Fazal , Nisar Momina , Ali Sajid , Elgorban Abdallah M. , Syed Asad , Elansary Hosam O. , El-Abedin Tarek K. Zin
Published: October 2022

The bactericidal effects of nanomaterials play an essential role in cytoplasmic leakage, leading to bacterial cell death. In this study, silver nanoparticles (AgNPs) were synthesized using a fungal extract of Aspergillus fumigatus (A. fumigatus). The physicochemical properties of the bare and myco-synthesized AgNPs (MS-AgNPs) were examined by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, ultraviolet–visible (UV–vis) spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). XRD revealed the crystalline structure of the prepared NPs. The FTIR spectrum of the MS-AgNPs revealed the presence of the stretching vibrations of hydroxyl (−OH) and carbonyl groups (C=O). The UV results showed absorption from 450 nm to 590 nm, confirming the synthesis of the AgNPs. SEM and TEM showed rough cubic shapes (spheres), 20–60 nm in size, while EDX confirmed the presence of 60% Ag in the sample. The MS-AgNPs revealed the highest antibacterial activity against Staphylococcus aureus, with a zone of inhibition of 18.21 ± 2.1 mm, followed by Shigella dysenteriae and Salmonella typhi. The bimetallic-AgNPs played a vital role in cell membrane damage and the release of cellular contents, specifically nucleic acids and proteins. These results suggest that MS-AgNPs have promising antimicrobial capabilities and might be beneficial for an extensive array of biological applications.
Laser-Induced Magnetization Dynamics in Si-Doped Yttrium-Iron Garnet Film
Authors: Gaponov Mikhail , Ovcharenko Sergey , Ilyin Nikita , Mishina Elena
Published: October 2022

We experimentally demonstrate that the excitation of a silicon-doped yttrium-iron garnet film by femtosecond laser pulses triggers a magnetization precession with an amplitude determined by the external magnetization direction. The maximum efficiency is achieved at the pump wavelength corresponding to the absorption maximum due to doping with silicon ions. Based on the azimuthal dependences of the precession amplitude and frequency, it is shown that the magnetization dynamics is induced by a thermal disruption of the magnetocrystalline anisotropy. By modeling hysteresis loops, it was found that the silicon doping leads to a decrease in the value of the exchange interaction in the film and an increase in the anisotropy field.
Thermophysical Properties of Sawdust and Coconut Coir Dust Incorporated Unfired Clay Blocks
Authors: Jannat Nusrat , Cullen Jeff , Abdullah Badr , Latif Al-Mufti Rafal , Karyono Karyono
Published: October 2022

Sawdust and coconut coir dust are agro-wastes/by-products which are suitable for use as raw materials to manufacture unfired clay blocks due to their excellent physical and mechanical properties. A limited number of studies have been conducted on the utilisation of these agro-wastes in clay block production, and they have mostly been devoted to investigating the physicomechanical properties, with less attention given to the thermal properties. Moreover, the majority of the studies have used chemical binders (cement and lime) in combination with agro-waste, thus increasing the carbon footprint and embodied energy of the samples. Furthermore, no research has been performed on the thermal performance of these agro-wastes when incorporated into clay blocks at the wall scale. Therefore, to address these limitations, the present study developed unfired clay blocks incorporating sawdust and coconut coir dust (0, 2.5, 5, and 7.5% by weight), without the use of chemical binders, and evaluated their thermal performance, both at the individual and wall scales. The experiments were divided into two phases. In the first phase, individual sample blocks was tested for basic thermal properties. Based on the results of the first phase, small walls with dimensions of 310 mm × 215 mm × 100 mm were built in the second phase, using the best performing mixture from each waste type, and these were assessed for thermal performance using an adapted hot box method. The thermal performance of the walls was evaluated by measuring the heat transfer rate from hot to cold environments and comparing the results to the reference wall. The results showed that thermal conductivity decreased from 0.36 W/mK for the reference sample, to 0.19 W/mK for the 7.5% coconut coir dust sample, and 0.21 W/mK for the 7.5% sawdust sample, indicating an improvement in thermal insulation. Furthermore, the coconut coir dust and sawdust sample walls showed a thermal resistance improvement of around 48% and 35%, respectively, over the reference sample wall. Consequently, the findings of this study will provide additional essential information that will help in assessing the prospective applications of sawdust and coconut coir dust as the insulating material for manufacturing unfired clay blocks.
Effect of Cold Atmospheric Plasma on SARS-CoV-2 Inactivation: A Pilot Study in the Hospital Environment
Authors: Loizou Constantinos , Kniazeva Volha , Apostolou Theofylaktos , Kornev Alexander , Kostevitch Serhei , Roslyakov Evgeny , Constantinou Costas , Hadjihannas Linos
Published: October 2022

The spread of the new coronavirus SARS-CoV-2 has substantial social, health, and economic impacts. High viral load in the air in hospitals poses a risk to medical personnel. Cold atmospheric plasma (CAP) is a new technology based on the emission in the air of reactive species, neutral particles, UV radiation, and electromagnetic field. CAP has the potential as an antiviral agent. In this study, an 80-day clinical trial took place at Nicosia General Hospital to evaluate the application of CAP devices for lowering the viral load in the COVID rooms. A total of 284 indoor environment samples were tested by RT-PCR, for which 9 were positive (~3% Positive Rate). After analyzing the initial results, an ion emitter was paired with each patient, and the results showed that the method could eliminate the virus from the COVID wards up to 100%. The number of patients discharged from the hospital in the ionizer group was 4.8% higher than in the non-ionizer group, and 45% fewer patients in the ionizer group who remained in the rooms required oxygen support. The clinical trial shows evidence that composite CAP can decrease coronavirus spread in hospital environments and potentially prevent virus transmission.
Changes over Time in Association Patterns between Estimated COVID-19 Case Fatality Rates and Demographic, Socioeconomic and Health Factors in the US States of Florida and New York
Authors: Joshi Mansi , Di Yanming , Bhattacharyya Sharmodeep , Chatterjee Shirshendu
Published: October 2022

The United States struggled exceptionally during the COVID-19 pandemic. For researchers and policymakers, it is of great interest to understand the risk factors associated with COVID-19 when examining data aggregated at a regional level. We examined the county-level association between the reported COVID-19 case fatality rate (CFR) and various demographic, socioeconomic and health factors in two hard-hit US states: New York and Florida. In particular, we examined the changes over time in the association patterns. For each state, we divided the data into three seasonal phases based on observed waves of the COVID-19 outbreak. For each phase, we used tests of correlations to explore the marginal association between each potential covariate and the reported CFR. We used graphical models to further clarify direct or indirect associations in a multivariate setting. We found that during the early phase of the pandemic, the association patterns were complex: the reported CFRs were high, with great variation among counties. As pandemics progressed, especially during the winter phase, socioeconomic factors such as median household income and health-related factors such as the prevalence of adult smokers and mortality rate of respiratory diseases became more significantly associated with the CFR. It is remarkable that common risk factors were identified for both states.
Hydrogen Embrittlement Behavior of Plastically Pre-Strained and Cathodically Hydrogen-Charged 316H Grade Austenitic Stainless Steel
Authors: Falat Ladislav , Čiripová Lucia , Petryshynets Ivan , Milkovič Ondrej , Džupon Miroslav , Kovaľ Karol
Published: October 2022

In this work, the effects of electrochemical hydrogen charging of 316H grade austenitic stainless steel were investigated in order to characterize its hydrogen embrittlement (HE) resistance. The as-received 316H material was in a fully recrystallized (solution-annealed) material condition. The susceptibility to HE of the studied material was evaluated by determination of the embrittlement index from the results of conventional uniaxial tensile tests of nonhydrogenated and hydrogen-charged test specimens. The study was focused on the effects of two selected plastic pre-strain levels of tensile specimens on their resulting HE resistance. The selected pre-strains corresponded to the tensile stress conditions within the “yield stress–ultimate tensile strength” (YS–UTS) range and directly at the UTS point. The obtained embrittlement indices for the presently used pre-straining and hydrogen charging conditions indicated that the HE of the studied material states was small. However, it was revealed that the observed degradation of deformation properties of plastically pre-strained and hydrogen-charged materials was mainly caused by gradual plasticity exhaustion due to tensile straining, which well correlated with the observed effects indicated by electron backscatter diffraction analyses and indentation hardness measurements.
Complementary Sampling Methods to Improve the Monitoring of Coastal Lagoons
Authors: Adao Ana C. , Bosch Néstor E. , Bentes Luís , Coelho Rui , Lino Pedro G. , Monteiro Pedro , Gonçalves Jorge M. S. , Erzini Karim
Published: October 2022

Monitoring the ecological status of marine coastal lagoons requires the integration of multiple indices. However, the efficacy of monitoring programs is complicated by the diverse array of habitats that conform coastal lagoons. In this study, we compared four sampling methods (25-m and 50-m beach seines, beam trawl and Riley push net) in the Ria Formosa coastal lagoon (South Portugal) for assessing fish assemblage and diversity. We compared species richness and assemblage structure with species accumulation curves and multivariate analysis, and assessed diversity patterns using taxonomic, phylogenetic and functional diversity indices. Variation in fish assemblage structure was mostly explained by gear type, and almost all diversity metrics varied not only according to sampling method but also depending on habitat characteristics and season. Based on operational costs and diversity patterns captured by each gear, we conclude that the combined use of 25-m beach seine and beam trawl is the preferred approach. The proposed methodology will provide the data necessary for assessment of ecological status of coastal lagoons.
A Novel Reverse Hinge Spoiler for Flight Loads Control
Authors: Ajaj Rafic , Djidjeli Kamal
Published: October 2022

This paper presents the reverse hinge spoiler, a novel spoiler concept, for flight load control. The reverse hinge spoiler is a control surface mounted on the upper surface of the wing. Unlike conventional hinged spoilers that are hinged at their front and rotate forward toward the leading edge of the wing, the proposed spoiler concept is hinged at its rear and rotates backward toward the trailing edge of the wing. The aerodynamic performance of the proposed spoiler is compared and contrasted with that of a conventional hinged spoiler for different flight conditions and hinge locations using the two-dimensional Reynolds-Averaged Navier–Stokes (RANS) with the k-omega SST turbulence model-based computational fluid dynamic solver. The results show that the proposed spoiler results in a larger increase in drag and a sharper reduction in the lift for a wide range of spoiler angles and flight conditions. Reversing the spoiler is found to cause a higher adverse pressure gradient in front of the spoiler compared to a conventional spoiler, as it ‘traps’ more flow, thereby increasing drag and reducing lift.
Is Omalizumab Related to Ear and Labyrinth Disorders? A Disproportionality Analysis Based on a Global Pharmacovigilance Database
Authors: Park Hyeon Tae , Park Sunny , Jung Yong Woo , Choi Soo An
Published: October 2022

Introduction: Asthma is a chronic disease, characterized by reversible airway obstruction, hypersensitivity reactions, and inflammation. Oral corticosteroids are an important treatment option for patients with severe or steroid-resistant asthma. Biologics for asthma are recommended in patients with severe asthma, owing to their steroid-sparing effect as well as their ability to reduce the severity and aggravation of uncontrolled asthma. Most clinical trials of omalizumab in patients with asthma have suggested its tolerability and safety. However, some studies reported eosinophilic comorbidities in the ear, nose, and throat during omalizumab treatment, particularly eosinophilic otitis media. This study examined the relationship between ear disorders and omalizumab compared with that of other biologics for asthma using a large real-world database. Materials and Methods: Individual case safety reports from the Uppsala Monitoring Centre Vigibase of biologics for asthma (omalizumab, mepolizumab, reslizumab, benralizumab, and dupilumab) up to 29 December 2019, were used. A disproportionality analysis was performed using the proportional reporting ratio (PRR), reporting odds ratio (ROR), and information components (IC). A hierarchy analysis used the Medical Dictionary for Regulatory Activities Terminology. A tree map was generated using R studio version 4.2. Results: In 32,618 omalizumab reports, 714 adverse events (AEs) were detected as signals. Among the 714 signals, seventeen AEs were detected as signals of omalizumab-related ear and labyrinth disorders in 394 reports. Only three AEs (ear pain, ear disorder, and ear discomfort) were detected from mepolizumab. No signal was detected from reslizumab, benralizumab, and dupilumab. Conclusions: Careful monitoring of ear disorders is recommended when omalizumab treatment is started, with decreased oral corticosteroid use in patients with severe asthma. Further studies are necessary to confirm the omalizumab-related signals.
The Potential Role of Artificial Intelligence in Lung Cancer Screening Using Low-Dose Computed Tomography
Authors: Grenier Philippe A. , Brun Anne Laure , Mellot François
Published: October 2022

Two large randomized controlled trials of low-dose CT (LDCT)-based lung cancer screening (LCS) in high-risk smoker populations have shown a reduction in the number of lung cancer deaths in the screening group compared to a control group. Even if various countries are currently considering the implementation of LCS programs, recurring doubts and fears persist about the potentially high false positive rates, cost-effectiveness, and the availability of radiologists for scan interpretation. Artificial intelligence (AI) can potentially increase the efficiency of LCS. The objective of this article is to review the performances of AI algorithms developed for different tasks that make up the interpretation of LCS CT scans, and to estimate how these AI algorithms may be used as a second reader. Despite the reduction in lung cancer mortality due to LCS with LDCT, many smokers die of comorbid smoking-related diseases. The identification of CT features associated with these comorbidities could increase the value of screening with minimal impact on LCS programs. Because these smoking-related conditions are not systematically assessed in current LCS programs, AI can identify individuals with evidence of previously undiagnosed cardiovascular disease, emphysema or osteoporosis and offer an opportunity for treatment and prevention.
Concordance Rate of Colposcopy in Detecting Cervical Intraepithelial Lesions
Authors: Stuebs Frederik A. , Dietl Anna K. , Behrens Annika , Adler Werner , Geppert Carol , Hartmann Arndt , Knöll Antje , Beckmann Matthias W. , Mehlhorn Grit , Schulmeyer Carla E. , Gass Paul , Koch Martin C.
Published: October 2022

Background: The purpose of this research is to estimate the rate of concordance, sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of colposcopy for high-grade squamous lesions and carcinomas (HSIL+). Methods: We conducted a retrospective study of colposcopies performed in the certified Dysplasia Unit in Erlangen between January 2015 and May 2022 (7.5 years). The colposcopic findings were correlated with biopsies obtained during examinations or surgery. Cases without histology were excluded. The primary outcome was the rate of concordance between the colposcopic and histological findings in relation to the type of transformation zone (TZ), examiner’s level of experience and age of the patients. Results: A total of 4778 colposcopies in 4001 women were analyzed. The rates of concordance for CIN I/LSIL, CIN II/HSIL, CIN III/HSIL, and carcinoma were 43.4%, 59.5%, 78.5%, and 53.9%, respectively. The rate of concordance was lowest for TZ3 and highest for colposcopists with more than 10 years’ experience. Conclusions: Colposcopy is an important, feasible, and effective method. Careful work-up needs to be performed for women with TZ3 who are over 35 years old, as they are at the highest risk of being misdiagnosed. The highest concordance for detecting HSIL+ was seen for colposcopists with >10 years’ experience.
Development of Advanced Imaging and Molecular Imaging for Barrett’s Neoplasia
Authors: Uno Kaname , Koike Tomoyuki , Hatta Waku , Saito Masahiro , Tanabe Mizuki , Masamune Atsushi
Published: October 2022

Barrett esophagus (BE) is a precursor to a life-threatening esophageal adenocarcinoma (EAC). Surveillance endoscopy with random biopsies is recommended for early intervention against EAC, but its adherence in the clinical setting is poor. Dysplastic lesions with flat architecture and patchy distribution in BE are hardly detected by high-resolution endoscopy, and the surveillance protocol entails issues of time and labor and suboptimal interobserver agreement for diagnosing dysplasia. Therefore, the development of advanced imaging technologies is necessary for Barrett’s surveillance. Recently, non-endoscopic or endoscopic technologies, such as cytosponge, endocytoscopy, confocal laser endomicroscopy, autofluorescence imaging, and optical coherence tomography/volumetric laser endomicroscopy, were developed, but most of them are not clinically available due to the limited view field, expense of the equipment, and significant time for the learning curve. Another strategy is focused on the development of molecular biomarkers, which are also not ready to use. However, a combination of advanced imaging techniques together with specific biomarkers is expected to identify morphological abnormalities and biological disorders at an early stage in the surveillance. Here, we review recent developments in advanced imaging and molecular imaging for Barrett’s neoplasia. Further developments in multiple biomarker panels specific for Barrett’s HGD/EAC include wide-field imaging systems for targeting ‘red flags’, a high-resolution imaging system for optical biopsy, and a computer-aided diagnosis system with artificial intelligence, all of which enable a real-time and accurate diagnosis of dysplastic BE in Barrett’s surveillance and provide information for precision medicine.
Prenatal Diagnosis of Right-Sided Congenital Ventricular Diverticulum (CVD) Assisted by Spatiotemporal Image Correlation (STIC) Acquisition and the Speckle-Tracking Technique to Assess Fetal Cardiac Function: A Case Report
Authors: Koh Ji Hye , Lee Hyo Jeong , Choi Jinyoung , Ahn Jun Woo
Published: October 2022

Congenital ventricular diverticulum (CVD) is a cardiac malformation defined as an outpouching lesion of a ventricle with normal contractility and thickness, and the advancement of prenatal sonography has led to its prenatal diagnosis. In the prenatal period, CVD is reported in association with pericardial effusion, arrhythmia, and fetal hydrops or as an isolated condition. With the development of prenatal echocardiography, CVD can be diagnosed from the early stage of pregnancy, and prenatal procedures, such as pericardiocentesis, are also possible. Spatiotemporal image correlation (STIC) acquisition, a novel approach for the clinical evaluation of fetal hearts, provides easy-to-use techniques for acquiring data from fetal hearts and helping visualization with two-dimensional and three-dimensional (3D) cine sequences. Furthermore, the speckle-tracking technique enables a more comprehensive evaluation of the shape, continuity, and function of the fetal heart. These recent techniques have never been used for CVD diagnosis and evaluation. Here, we present a case of right-sided CVD, which is the first in which STIC acquisition and cardiac function assessment with the speckle-tracking technique were used to assist in the diagnosis and evaluation.
Swiss-Chinese Cooperation in Tropical Medicine: The Role of Professor Marcel Tanner
Authors: Lv Shan , Ding Wei , Bergquist Robert , Yang Guojing , Guo Jiagang , Zhou Xiao-Nong
Published: October 2022

This paper is in honour of Professor Marcel Tanner, President of the Swiss Academies of Arts and Sciences, and former Director of the Swiss Tropical and Public Health Institute (Swiss TPH), in Basel, Switzerland. In the 30 plus years since his first visit to China in 1989, Professor Tanner has tirelessly promoted research collaboration between Switzerland and China on health and tropical diseases through international meetings, scholar exchange, and training of young scientists. As a contribution to Professor Tanner’s life’s work of collaboration with Chinese scientists, we summarize here ideas conceived, work initiated and major outcomes. His approach, embodied in his flowery expression: “Alps and Himalayas never meet, but Swiss and Chinese can”, marked the occasion in 2013 when Xinhua Co., Ltd., a pharmaceutical company in Shandong of China, agreed to produce tribendimidine, a new remedy for tropical helminth infections, that was the fruit of long-term research by scientists at the Swiss TPH in Basel, and National Institute of Parasitic Diseases (NIPD) in Shanghai. This was neither the first nor the last of Professor Tanner’s forceful, yet diplomatic influence, and we follow in his footprints when continuing in Swiss-Chinese cooperation in tropical medicine.
Adaptive Signal Processing and Machine Learning Using Entropy and Information Theory
Authors: Ogunfunmi Tokunbo
Published: October 2022

This Special Issue on “Adaptive Signal Processing and Machine Learning Using Entropy and Information Theory” was birthed from observations of the recent trend in the literature [...]
A Quantile General Index Derived from the Maximum Entropy Principle
Authors: Sei Tomonari
Published: October 2022

We propose a linear separation method of multivariate quantitative data in such a way that the average of each variable in the positive group is larger than that of the negative group. Here, the coefficients of the separating hyperplane are restricted to be positive. Our method is derived from the maximum entropy principle. The composite score obtained as a result is called the quantile general index. The method is applied to the problem of determining the top 10 countries in the world based on the 17 scores of the Sustainable Development Goals (SDGs).
Geometric Partition Entropy: Coarse-Graining a Continuous State Space
Authors: Diggans Christopher Tyler , AlMomani Abd AlRahman R.
Published: October 2022

Entropy is re-examined as a quantification of ignorance in the predictability of a one dimensional continuous phenomenon. Although traditional estimators for entropy have been widely utilized in this context, we show that both the thermodynamic and Shannon’s theory of entropy are fundamentally discrete, and that the limiting process used to define differential entropy suffers from similar problems to those encountered in thermodynamics. In contrast, we consider a sampled data set to be observations of microstates (unmeasurable in thermodynamics and nonexistent in Shannon’s discrete theory), meaning, in this context, it is the macrostates of the underlying phenomenon that are unknown. To obtain a particular coarse-grained model we define macrostates using quantiles of the sample and define an ignorance density distribution based on the distances between quantiles. The geometric partition entropy is then just the Shannon entropy of this finite distribution. Our measure is more consistent and informative than histogram-binning, especially when applied to complex distributions and those with extreme outliers or under limited sampling. Its computational efficiency and avoidance of negative values can also make it preferable to geometric estimators such as k-nearest neighbors. We suggest applications that are unique to this estimator and illustrate its general utility through an application to time series in the approximation of an ergodic symbolic dynamics from limited observations.
The Effects of Machinima on Communication Skills in Students with Developmental Dyslexia
Authors: Pellas Nikolaos , Christopoulos Athanasios
Published: October 2022

Many research efforts in the international literature have been conducted to investigate various fundamental issues associated with communication skills cultivation of students with developmental dyslexia. However, little is known when it comes to the impact that ‘immersive technologies’, such as three-dimensional virtual worlds, without considering any exploration of their impact to assist boys and girls with developmental dyslexia cultivate communication skills. Motivated by this inadequacy in the literature, the purpose of this study is to explore the effectiveness of the machinima approach, created via OpenSimulator and Scratch4SL, for students with developmental dyslexia in vocabulary learning and practicing. This embedded mixed-methods research was conducted over a four-week timetable in-class course, with forty students (n = 40) aged 10–12 years old. All students were equally separated into two groups in line with their gender. Boys and girls were encouraged to unfold the communication skills developed (i.e., spelling, writing, reading) by creating their own stories, after viewing educational videos and machinima scenes, before and after the treatment. The results indicate that machinima positively affected students’ learning outcomes and achievements. Machinima can improve immediate knowledge gains in boys compared to girls to purposefully translate their cognitive thinking into storytelling, when problem-solving situations through simulated realism are considered. This study also offers insights for educational implications and design guidelines for machinima creation, providing empirical evidence on its effect on the participants’ linguistics understanding and communication skills for language learning in girls and boys with developmental dyslexia.
Recent Advances in the Electro-Active Therapeutic Phytochemical-Based Sensors
Authors: Gandhi Mansi , Amreen Khairunnisa , Tiwari Brahm Kumar
Published: October 2022

Naturally occurring phytonutrients/phyto-components are likely to have therapeutic values. These phyto-derived naturally occurring components, such as polyphenols, phenolics, flavonoids and phenolic acids have a hydrocarbon background with a polyphenolic ring, an ester bond with a polyphenolic ring, etc. Their structures play a critical role in determining the chemical and physical attributes that define their activity/functions and roles. Owing to their chemical structure, most of them are electroactive. Thus, these phytochemicals can be used in the preparation of electrochemical sensors. Gaining an understanding of functional genotypical units using electrochemistry is a unique study. The feasibility of incorporating an array of biosensors into a fully-automated micro-electrochemical system is further explored. This review is intended to provide in-depth knowledge of biosensors’ applications based on/for Plantae kingdom and varieties. The discussion focuses primarily on the fields associated with the fully-automated micro-electrochemical system and appropriate methods for its advancement. The intended approach is to provide a selective outlook including the setbacks/shortcomings and usefulness of opting for the concerned technique.
Thermal Annealing Effect on the Structure, Optical and Electrical Properties of Lanthanum Manganite Thin Films Prepared by Reactive Co-Sputtering
Authors: Hourani Wael , Rousselot Christophe , N’Djoré Kouamé Boko Joël-Igor , Billard Alain , Arab Pour Yazdi Mohammad , Makoudi Younes
Published: October 2022

Lanthanum manganite (LMO) thin films were deposited by co-sputtering La and Mn targets in an Ar and O2 gas mixture. The films were synthesized on silicon and fused silica substrates. The influences of thermal annealing on the structure, optical and electrical properties of LMO films were investigated. The results exhibited a correlation between these properties. In the amorphous state, an increase in annealing temperature improved the optical transmission and decreased the electrical capacitance. The beginning of crystallization at 600 °C was manifested by a strong increase in the capacitance and a decrease in the optical transmission. At higher annealing temperature, polycrystalline films were obtained with different optical and electrical characteristics. On the other hand, the annealed LMO films showed a photocurrent effect during exposure to a weak LED light.
Textile Materials for Wireless Energy Harvesting
Authors: Yamada Yusuke
Published: October 2022

Wireless energy harvesting, a technique to generate direct current (DC) electricity from ambient wireless signals, has recently been featured as a potential solution to reduce the battery size, extend the battery life, or replace batteries altogether for wearable electronics. Unlike other energy harvesting techniques, wireless energy harvesting has a prominent advantage of ceaseless availability of ambient signals, but the common form of technology involves a major challenge of limited output power because of a relatively low ambient energy density. Moreover, the archetypal wireless energy harvesters are made of printed circuit boards (PCBs), which are rigid, bulky, and heavy, and hence they are not eminently suitable for body-worn applications from both aesthetic and comfort points of view. In order to overcome these limitations, textile-based wireless energy harvesting architectures have been proposed in the past decade. Being made of textile materials, this new class of harvesters can be seamlessly integrated into clothing in inherently aesthetic and comfortable forms. In addition, since clothing offers a large surface area, multiple harvesting units can be deployed to enhance the output power. In view of these unique and irreplaceable benefits, this paper reviews key recent progress in textile-based wireless energy harvesting strategies for powering body-worn electronics. Comparisons with other power harvesting technologies, historical development, fundamental principles of operation and techniques for fabricating textile-based wireless power harvesters are first recapitulated, followed by a review on the principal advantages, challenges, and opportunities. It is one of the purposes of this paper to peruse the current state-of-the-art and build a scientific knowledge base to aid further advancement of power solutions for wearable electronics.
Hybrid Full Adders: Optimized Design, Critical Review and Comparison in the Energy-Delay Space
Authors: Giustolisi Gianluca , Palumbo Gaetano
Published: October 2022

In this paper, we design and compare seven meaningful hybrid one-bit full adders topologies that are optimized in terms of energy-delay trade-offs to operate in multibit ripple carry adders. The goal is to provide the designer with a simple and powerful approach for choosing the best topology for a given power budget, speed performance, or any combination of both. The design and comparison deal with 4-bit and 8-bit ripple carry adders and exploit the derivation of the energy-efficient curves in the energy-delay space. To do so, first we define the procedures to obtain energy consumption and propagation delay by simulating a ripple carry adder designed at a transistor level. Then, we introduce a design methodology to optimize a ripple carry adder by minimizing some significant figures-of-merit in terms of energy-delay trade-offs. The comparison of the energy-efficient curves allows us to make a simple and effective comparison as well as to identify the best one-bit full adder topologies.
Comparison of Grid Reactive Voltage Regulation with Reconfiguration Network for Electric Vehicle Penetration
Authors: Nagi Farrukh , Azwin Aidil , Boopalan Navaamsini , Ramasamy Agileswari K. , Marsadek Marayati , Ahmed Syed Khaleel
Published: October 2022

Renewable energy sources and EV growth brings new challenges for grid stabilization. Smart grid techniques are required to reconfigure and compensate for load fluctuation and stabilize power losses and voltage fluctuation. Numerical tools are available to equip the smart grid to deal with such challenges. Distribution Feeder reconfiguration and reactive voltage injection to the disturbed grid are some of the techniques employed for the purpose. However, either reconfiguration or injection alone is used commonly for this purpose. In this study, both techniques are applied to EV penetration as load and compared. A balanced IEEE 33 Radial network is used in this study and selected branches with high power losses are targeted for the reactive voltage injection and Minimum Spanning tree techniques (MST). EV charging loads are usually modelled with time base distribution which requires times base power flow analysis for reactive power injection. A comparison between coordinated, reconfiguration, and reactive voltage injection shows differences in power losses, voltage distortion, and cost saving. The analysis is carried out with an integer linear programming technique for coordinated charging, a minimum spanning tree for network reconfiguration, and genetic optimization for reactive power injection. Besides, all power flow analyses are carried out with the Backward/Forward sweep method. The information would help lowering power losses, grid stabilization, and charging station infrastructure planning.