bluuebunny/crossref_metadata_2025 huggingface dataset:
Amelioration of the Oxidative Stress Generated by Simple or Combined Abiotic Stress through the K+ and Ca2+ Supplementation in Tomato Plants
Authors: García-Martí María , Piñero María Carmen , García-Sanchez Francisco , Mestre Teresa C. , López-Delacalle María , Martínez Vicente , Rivero Rosa M.
Published: April 2019

Abiotic stressors such as drought, heat, or salinity are major causes of yield loss worldwide due to the oxidative burst generated under these conditions. Recent studies have revealed that plant response to a combination of different environmental stressors is unique and cannot be deduced from the response developed to each stress when applied individually. Some studies have demonstrated that a different management of some nutrients in the irrigation solution may provide an advantage to the plants against abiotic stressors. Thus, the aim of this study was to investigate if an increase in potassium (K+) and calcium (Ca2+) concentration in the nutrient solution may have a positive effect on the amelioration of oxidative stress which occurs under the combination of salinity and heat in tomato plants. Our results indicated that plants irrigated with an increase in K+ and Ca2+ concentrations in the irrigation solution from 7mM (K+) to 9.8 mM and from 4 mM (Ca2+) to 5.6 mM, respectively, induced a recovery of the biomass production compared to the plants treated with salinity or salinity + heat, and subsequently irrigated with the regular Hoagland solution. This was correlated with a better performance of all the photosynthetic parameters, a reduction in the foliar concentration of H2O2 and a lower lipid peroxidation rate, and with a better performance of the antioxidant enzymes ascorbate peroxidase ascorbate peroxidase (APX), dehydroascorbate reductactase (DHAR), glutathione reductase (GR), and NADPH oxidase. Our results showed that these enzymes were differentially regulated at the transcriptional level, showing a higher reactive oxygen species (ROS) detoxification efficiency under salinity and under the combination of salinity and heat, as compared to those plants irrigated with common Hoagland. An increase in K+ and Ca2+ in the irrigation solution also induced a lower Na+ accumulation in leaves and a higher K+/Na+ ratio. Thus, our study highlights the importance of the right management of the plant nutritional status and fertilization in order to counteract the deleterious effects of abiotic stress in plants.
Protective Effect of Glutathione against Oxidative Stress-induced Cytotoxicity in RAW 264.7 Macrophages through Activating the Nuclear Factor Erythroid 2-Related Factor-2/Heme Oxygenase-1 Pathway
Authors: Kwon Da Hye , Cha Hee-Jae , Lee Hyesook , Hong Su-Hyun , Park Cheol , Park Shin-Hyung , Kim Gi-Young , Kim Suhkmann , Kim Heui-Soo , Hwang Hye-Jin , Choi Yung Hyun
Published: April 2019

Reactive oxygen species (ROS), products of oxidative stress, contribute to the initiation and progression of the pathogenesis of various diseases. Glutathione is a major antioxidant that can help prevent the process through the removal of ROS. The aim of this study was to evaluate the protective effect of glutathione on ROS-mediated DNA damage and apoptosis caused by hydrogen peroxide, H2O2, in RAW 264.7 macrophages and to investigate the role of the nuclear factor erythroid 2-related factor-2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway. The results showed that the decrease in the survival rate of RAW 264.7 cells treated with H2O2 was due to the induction of DNA damage and apoptosis accompanied by the increased production of ROS. However, H2O2-induced cytotoxicity and ROS generation were significantly reversed by glutathione. In addition, the H2O2-induced loss of mitochondrial membrane potential was related to a decrease in adenosine triphosphate (ATP) levels, and these changes were also significantly attenuated in the presence of glutathione. These protective actions were accompanied by a increase in the expression rate of B-cell lymphoma-2 (Bcl-2)/Bcl-2-associated X protein (Bax) and poly(ADP-ribose) polymerase cleavage by the inactivation of caspase-3. Moreover, glutathione-mediated cytoprotective properties were associated with an increased activation of Nrf2 and expression of HO-1; however, the inhibition of the HO-1 function using an HO-1 specific inhibitor, zinc protoporphyrin IX, significantly weakened the cytoprotective effects of glutathione. Collectively, the results demonstrate that the exogenous administration of glutathione is able to protect RAW 264.7 cells against oxidative stress-induced mitochondria-mediated apoptosis along with the activity of the Nrf2/HO-1 signaling pathway.
Investigating Quantum Coherence by Negative Excursions of the Wigner Quasi-Distribution
Authors: Ballicchia Mauro , Ferry David K. , Nedjalkov Mihail , Weinbub Josef
Published: April 2019

Quantum information and quantum communication are both strongly based on concepts of quantum superposition and entanglement. Entanglement allows distinct bodies, that share a common origin or that have interacted in the past, to continue to be described by the same wave function until evolution is coherent. So, there is an equivalence between coherence and entanglement. In this paper, we show the relation between quantum coherence and quantum interference and the negative parts of the Wigner quasi-distribution, using the Wigner signed-particle formulation. A simple physical problem consisting of electrons in a nanowire interacting with the potential of a repulsive dopant placed in the center of it creates a quasi two-slit electron system that separates the wave function into two entangled branches. The analysis of the Wigner quasi-distribution of this problem establishes that its negative part is principally concentrated in the region after the dopant between the two entangled branches, maintaining the coherence between them. Moreover, quantum interference is shown in this region both in the positive and in the negative part of the Wigner function and is produced by the superposition of Wigner functions evaluated at points of the momentum space that are symmetric with respect to the initial momentum of the injected electrons.
Experimental Study on the Fracture Process Zone Characteristics in Concrete Utilizing DIC and AE Methods
Authors: Dai Shuhong , Liu Xiaoli , Nawnit Kumar
Published: April 2019

The present work focuses on investigating the characteristics of the fracture process zone (FPZ) in concrete. The Single-edge notched (SEN) concrete beams under three-points bending are employed for conducting mode I fracture propagation. The displacement fields on the specimen surface and the internal AE signal of specimen are obtained simultaneously in real time by digital image correlation (DIC) and acoustic emission (AE) techniques. The experimental and analytical results indicated that the crack tip position, the crack extension length and the stress intensity factors (SIF) are obtained dynamically and quantitatively by DIC technique, and the length of FPZ is identified, respectively, by DIC and AE techniques in the crack extension process. The distribution of internal AE events is consistent with that of FPZ identified from surface deformation of specimens.
Water Quality Changes in Cement-Lined Water Pipe Networks
Authors: Wąsowski Jacek , Kowalski Dariusz , Kowalska Beata , Kwietniewski Marian , Zawilska Małgorzata
Published: April 2019

This research focused on a model setup fed with underground water pumped into a water supply network. The scope of the research included a long- and a short-term stagnation of water in the setup and a water flow of 0.1 m/s. The water supplied into the system gradually lost its corrosive properties and developed calcium carbonate precipitation capability. These processes indicated that cement components migrate from the lining into the water. Apart from the primary components, the presence of the following trace elements was found: iron (399.6 ppb), magnesium (17.8 ppm), zinc (26.9 ppb), barium (22.6 ppb), boron (21.6 ppb), sodium (10.8 ppm), gallium (2.1 ppb). At early stages of the operation, recorded quantities were not significant. Leaching of the trace elements from cement increased after water was chlorinated. In turn, the highest percent increases were observed in the case of zinc (217%), boron (19%) and gallium (12%), whereas the increase for sodium, iron, barium and magnesium in water was insignificant (reaching a few percent). A repeated analysis of samples of stagnant water in the setup showed that most of trace elements were below the detectability level. Final concentrations of all elements identified in the water after its contact with internal cement mortar lining were much lower than the limit values set out for drinking water.
A New Method of Simultaneous Localization and Mapping for Mobile Robots Using Acoustic Landmarks
Authors: Chen Xiaohui , Sun Hao , Zhang Heng
Published: April 2019

The simultaneous localization and mapping (SLAM) problem for mobile robots has always been a hotspot in the field of robotics. Simultaneous localization and mapping for robots using visual sensors and laser radar is easily affected by the field of view and ground conditions. According to the problems of traditional sensors applied in SLAM, this paper presents a novel method to perform SLAM using acoustic signals. This method enables robots equipped with sound sources, moving within a working environment and interacting with microphones of interest, to locate itself and map the objects simultaneously. In our case, a method of microphone localization based on a sound source array is proposed, and it was applied as a pre-processing step to the SLAM procedure. A microphone capable of receiving sound signals can be directly used as a feature landmark of a robot observation model without feature extraction. Meanwhile, to eliminate the random error caused by hardware equipment, a sound settled in the middle of two microphones was applied as a calibration sound source to determine the value of the random error. Simulations and realistic experimental results demonstrate the feasibility and effectiveness of the proposed method.
Three-Dimensional Physical and Numerical Modelling of Fracturing and Deformation Behaviour of Mining-Induced Rock Slopes
Authors: Yang Guoxiang , Leung Anthony K. , Xu Nengxiong , Zhang Kunxiang , Gao Kunpeng
Published: April 2019

Fracturing behaviour of jointed rock mass subjected to mining can significantly affect the stability of the rock structures and rock slopes. Ore mining within an open-pit final slope would lead to large-scale strata and surface movement of the rock slope. Rock mass structure, or more specifically, the strength, spacing and distribution of rock joints, are the controlling factors that govern the failure and deformation mechanisms of the final slope. Two-dimensional (2-D) physical modelling tests have been conducted in the literature, but in general, most of them have simplified the geological conditions and neglected some key features of rock mass structure in the field. In this study, new three-dimensional (3-D) physical modelling methods are introduced, with realistic modelling of mechanical behaviour of rock mass as well as identified properties of predominant rock joint sets. A case study of Yanqianshan iron mine is considered and the corresponding 1:200 model rock slope was created for studying the rock joint effects on the strata movement and the subsidence mechanism of the slope. The physical model test results are subsequently verified with 3-D discrete element numerical modelling. Due to the presence of the predominant joints, the observed well-shaped strata subsidence in Yanqianshan iron mine was successfully reproduced in the 3-D physical model. The failure mechanism of rock slopes differs from the trumpet-shaped subsidence observed in unconsolidated soil. Due to the formation of an arching mechanism within the rock mass, the strata deformation transferred gradually from the roof of the goaf to the slope surface.
SDD-CNN: Small Data-Driven Convolution Neural Networks for Subtle Roller Defect Inspection
Authors: Xu Xiaohang , Zheng Hong , Guo Zhongyuan , Wu Xiongbin , Zheng Zhaohui
Published: April 2019

Roller bearings are some of the most critical and widely used components in rotating machinery. Appearance defect inspection plays a key role in bearing quality control. However, in real industries, bearing defects are usually extremely subtle and have a low probability of occurrence. This leads to distribution discrepancies between the number of positive and negative samples, which makes intelligent data-driven inspection methods difficult to develop and deploy. This paper presents a small data-driven convolution neural network (SDD-CNN) for roller subtle defect inspection via an ensemble method for small data preprocessing. First, label dilation (LD) is applied to solve the problem of an imbalance in class distribution. Second, a semi-supervised data augmentation (SSDA) method is proposed to extend the dataset in a more efficient and controlled way. In this method, a coarse CNN model is trained to generate ground truth class activation and guide the random cropping of images. Third, four variants of the CNN model, namely, SqueezeNet v1.1, Inception v3, VGG-16, and ResNet-18, are introduced and employed to inspect and classify the surface defects of rollers. Finally, a rich set of experiments and assessments is conducted, indicating that these SDD-CNN models, particularly the SDD-Inception v3 model, perform exceedingly well in the roller defect classification task with a top-1 accuracy reaching 99.56%. In addition, the convergence time and classification accuracy for an SDD-CNN model achieve significant improvement compared to that for the original CNN. Overall, using an SDD-CNN architecture, this paper provides a clear path toward a higher precision and efficiency for roller defect inspection in smart manufacturing.
Effect of Vinyl Flooring on the Modal Properties of a Steel Footbridge
Authors: Jiménez-Alonso Javier Fernando , Pérez-Aracil Jorge , Hernández Díaz Alejandro Mateo , Sáez Andrés
Published: April 2019

Damping ratios associated with non-structural elements play an important role in mitigating the pedestrian-induced vibrations of slender footbridges. In particular, this paper analyses the effect of vinyl flooring on the modal parameters of steel footbridges. Motivated by the unexpected high experimental damping ratios of the first vibration modes of a real footbridge, whose deck was covered by a vinyl flooring, this paper aims at assessing more accurately the experimental damping ratios generated by this non-structural element on steel footbridges. For this purpose, a laboratory footbridge was built and vinyl flooring was installed on it. Its numerical and experimental modal parameters without and with the vinyl flooring were determined. The operational modal analysis method was used to estimate experimentally the modal parameters of the structure. The damping ratios associated with the vinyl flooring were obtained via the substraction between the experimental damping ratios of the laboratory footbridge with and without the vinyl flooring. An average increase of the damping ratios of 2.069% was observed due to the vinyl flooring installed. According to this result, this type of pavement may be a useful tool to significantly increase the damping ratios of steel footbridges in order to reduce pedestrian-induced vibrations.
A Contactless Measuring Method of Skin Temperature based on the Skin Sensitivity Index and Deep Learning
Authors: Cheng Xiaogang , Yang Bin , Tan Kaige , Isaksson Erik , Li Liren , Hedman Anders , Olofsson Thomas , Li Haibo
Published: April 2019

In human-centered intelligent building, real-time measurements of human thermal comfort play critical roles and supply feedback control signals for building heating, ventilation, and air conditioning (HVAC) systems. Due to the challenges of intra- and inter-individual differences and skin subtleness variations, there has not been any satisfactory solution for thermal comfort measurements until now. In this paper, a contactless measuring method based on a skin sensitivity index and deep learning (NISDL) was proposed to measure real-time skin temperature. A new evaluating index, named the skin sensitivity index (SSI), was defined to overcome individual differences and skin subtleness variations. To illustrate the effectiveness of SSI proposed, a two multi-layers deep learning framework (NISDL method I and II) was designed and the DenseNet201 was used for extracting features from skin images. The partly personal saturation temperature (NIPST) algorithm was use for algorithm comparisons. Another deep learning algorithm without SSI (DL) was also generated for algorithm comparisons. Finally, a total of 1.44 million image data was used for algorithm validation. The results show that 55.62% and 52.25% error values (NISDL method I, II) are scattered at (0 °C, 0.25 °C), and the same error intervals distribution of NIPST is 35.39%.
A Hardware-Efficient Vector Quantizer Based on Self-Organizing Map for High-Speed Image Compression. Appl. Sci. 2017, 7, 1106
Authors: Huang Zunkai , Suzuki Dai , Zhang Xiangyu , Chen Lei , Zhu Yongxin , An Fengwei , Wang Hui , Feng Songlin , Mattausch Hans
Published: April 2019

We, the authors, wish to make the following corrections to our published paper [...]
A Review of the Representation of Aerosol Mixing State in Atmospheric Models
Authors: Stevens Robin , Dastoor Ashu
Published: April 2019

Aerosol mixing state significantly affects concentrations of cloud condensation nuclei (CCN), wet removal rates, thermodynamic properties, heterogeneous chemistry, and aerosol optical properties, with implications for human health and climate. Over the last two decades, significant research effort has gone into finding computationally-efficient methods for representing the most important aspects of aerosol mixing state in air pollution, weather prediction, and climate models. In this review, we summarize the interactions between mixing-state and aerosol hygroscopicity, optical properties, equilibrium thermodynamics and heterogeneous chemistry. We focus on the effects of simplified assumptions of aerosol mixing state on CCN concentrations, wet deposition, and aerosol absorption. We also summarize previous approaches for representing aerosol mixing state in atmospheric models, and we make recommendations regarding the representation of aerosol mixing state in future modelling studies.
A Comparison of North American Surface Temperature and Temperature Extreme Anomalies in Association with Various Atmospheric Teleconnection Patterns
Authors: Yu Bin , Lin Hai , Soulard Nicholas
Published: April 2019

The atmospheric teleconnection pattern reflects large-scale variations in the atmospheric wave and jet stream, and has pronounced impacts on climate mean and extremes over various regions. This study compares those patterns that have significant circulation anomalies over the North Pacific–North American–North Atlantic sector, which directly influence surface temperature and temperature extremes over North America. We analyze the pattern associated anomalies of surface temperature and warm and cold extremes over North America, during the northern winter and summer seasons. In particular, we assess the robustness of the regional temperature and temperature extreme anomaly patterns by evaluating the field significance of these anomalies over North America, and quantify the percentages of North American temperature and temperature extreme variances explained by these patterns. The surface temperature anomalies in association with the Pacific–North American pattern (PNA), Tropical–Northern Hemisphere pattern (TNH), North Pacific pattern (NP), North Atlantic Oscillation (NAO), Arctic Oscillation (AO), Western Pacific pattern (WP), circumglobal teleconnection (CGT), and Asian–Bering–North American (ABNA) patterns are similar to those reported in previous studies based on various datasets, indicating the robustness of the results. During winter, the temperature anomaly patterns considered are field significant at the 5% level over North America, except the WP-related one. These pattern associated anomalies explained about 5–15% of the total interannual temperature variance over North America, with relatively high percentages for the ABNA and PNA patterns, and low for the WP pattern. The pattern associated warm and cold extreme anomalies resemble the corresponding surface mean temperature anomaly patterns, with differences mainly in magnitude of the anomalies. Most of the anomalous extreme patterns are field significant at the 5% level, except the WP-related patterns. These extreme anomalies explain about 5–20% of the total interannual variance over North America. During summer, the pattern-related circulation and surface temperature anomalies are weaker than those in winter. Nevertheless, all of the pattern associated temperature anomalies are of field significance at the 5% level over North America, except the PNA-related one, and explain about 5–10% of the interannual variance. In addition, the temperature extreme anomalies, in association with the circulation patterns, are comparable in summer and winter. Over North America, the NP-, WP-, ABNA-, and CGT-associated anomalies of warm extremes are field significant at the 5% level and explain about 5–15% of the interannual variance. Most of the pattern associated cold extreme anomalies are field significant at the 5% level, except the PNA and NAO related anomalies, and also explain about 5–15% of the interannual variance over North America.
Factors that May Protect the Native Hibernator Syrian Hamster Renal Tubular Epithelial Cells from Ferroptosis Due to Warm Anoxia-Reoxygenation
Authors: Eleftheriadis Theodoros , Pissas Georgios , Liakopoulos Vassilios , Stefanidis Ioannis
Published: April 2019

Warm anoxia-reoxygenation induces ferroptotic cell death in mouse proximal renal tubular epithelial cells (RPTECs), whereas RPTECs of the native hibernator Syrian hamster resist cell death. Clarifying how hamster cells escape ferroptosis may reveal new molecular targets for preventing or ameliorating ischemia-reperfusion-induced human diseases or expanding the survival of organ transplants. Mouse or hamster RPTECs were subjected to anoxia and subsequent reoxygenation. Cell death was assessed with the lactated dehydrogenase (LDH) release assay and lipid peroxidation by measuring cellular malondialdehyde (MDA) fluorometrically. The effect of the ferroptosis inhibitor α-tocopherol on cell survival was assessed by the 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) assay. The expression of the critical ferroptotic elements cystine-glutamate antiporter (xCT), ferritin, and glutathione peroxidase 4 (GPX4) was assessed by Western blot. Contrary to mouse RPTECs, hamster RPTECs resisted anoxia-reoxygenation-induced cell death and lipid peroxidation. In mouse RPTECs, α-tocopherol increased cell survival. Anoxia increased the levels of xCT, ferritin, and GPX4 in both cell types. During reoxygenation, at which reactive oxygen species overproduction occurs, xCT and ferritin decreased, whereas GPX4 increased in mouse RPTECs. In hamster RPTECs, reoxygenation raised xCT and ferritin, but lowered GPX4. Hamster RPTECs resist lipid peroxidation-induced cell death. From the three main evaluated components of the ferroptotic pathway, the increased expression of xCT and ferritin may contribute to the resistance of the hamster RPTECs to warm anoxia-reoxygenation.
Splicing Factors Have an Essential Role in Prostate Cancer Progression and Androgen Receptor Signaling
Authors: Takayama Ken-ichi
Published: April 2019

Although inhibition of the androgen–androgen receptor (AR) axis effectively represses the growth of prostate cancer, most of all cases eventually become castration-resistant prostate cancers (CRPCs). Enhancement of the expression of AR and its variants along with the downstream signals is important for disease progression. AR-V7, a constitutive active form of AR, is generated as a result of RNA splicing. RNA splicing creates multiple transcript variants from one pre-messenger RNA (mRNA) by removing introns/exons to allow mRNA translation. The molecular mechanisms leading to marked increases of AR and generation of AR-V7 have been unclear. However, recent papers highlighted the roles of RNA splicing factors which promote AR expression and production of variants. Notably, a broad range of splicing components were aberrantly regulated in CRPC tissues. Interestingly, expression of various spliceosome genes is enhanced by RNA-binding protein splicing factor proline- and glutamine-rich (PSF/SFPQ), leading to changes in the expression of AR transcript variants. Moreover, inhibition of several splicing factors repressed tumor growth in vivo. Altered expression of splicing factors is correlated to biochemical recurrence in prostate cancer patients. Thus, these findings suggest that splicing factors would be a potential therapeutic target. This review focuses on the emerging roles of splicing factors in prostate cancer progression and AR signaling.
Optimization of the Electrodeposition of Gold Nanoparticles for the Application of Highly Sensitive, Label-Free Biosensor
Authors: Chiang Hao-Chun , Wang Yanyan , Zhang Qi , Levon Kalle
Published: April 2019

A highly sensitive electrochemical biosensor with a signal amplification platform of electrodeposited gold nanoparticle (AuNP) has been developed and characterized. The sizes of the synthesized AuNP were found to be critical for the performance of biosensor in which the sizes were dependent on HAuCl4 and acid concentrations; as well as on scan cycles and scan rates in the gold electro-reduction step. Systematic investigations of the adsorption of proteins with different sizes from aqueous electrolyte solution onto the electrodeposited AuNP surface were performed with a potentiometric method and calibrated by design of experiment (DOE). The resulting amperometric glucose biosensors was demonstrated to have a low detection limit (>50 μM) and a wide linear range after optimization with AuNP electrodeposition.
CX Chemokine Receptor 7 Contributes to Survival of KRAS-Mutant Non-Small Cell Lung Cancer upon Loss of Epidermal Growth Factor Receptor
Authors: Liu Bin , Song Shanshan , Setroikromo Rita , Chen Siwei , Hu Wenteng , Chen Deng , van der Wekken Anthonie J. , Melgert Barbro N. , Timens Wim , van den Berg Anke , Saber Ali , Haisma Hidde J.
Published: April 2019

KRAS-driven non-small cell lung cancer (NSCLC) patients have no effective targeted treatment. In this study, we aimed to investigate targeting epidermal growth factor receptor (EGFR) as a therapeutic approach in KRAS-driven lung cancer cells. We show that ablation of EGFR significantly suppressed tumor growth in KRAS-dependent cells and induced significantly higher expression of CX chemokine receptor 7 (CXCR7) and activation of MAPK (ERK1/2). Conversely, rescue of EGFR led to CXCR7 downregulation in EGFR−/− cells. Dual EGFR and CXCR7 inhibition led to substantial reduction of MAPK (pERK) and synergistic inhibition of cell growth. Analysis of two additional EGFR knockout NSCLC cell lines using CRISPR/Cas9 revealed genotype dependency of CXCR7 expression. In addition, treatment of different cells with gefitinib increased CXCR7 expression in EGFRwt but decreased it in EGFRmut cells. CXCR7 protein expression was detected in all NSCLC patient samples, with higher levels in adenocarcinoma as compared to squamous cell lung carcinoma and healthy control cases. In conclusion, EGFR and CXCR7 have a crucial interaction in NSCLC, and dual inhibition may be a potential therapeutic option for NSCLC patients.
Circulating Cell-Free DNA and RNA Analysis as Liquid Biopsy: Optimal Centrifugation Protocol
Authors: Sorber Laure , Zwaenepoel Karen , Jacobs Julie , De Winne Koen , Goethals Sofie , Reclusa Pablo , Van Casteren Kaat , Augustus Elien , Lardon Filip , Roeyen Geert , Peeters Marc , Van Meerbeeck Jan , Rolfo Christian , Pauwels Patrick
Published: April 2019

The combined analysis of circulating cell-free (tumor) DNA (cfDNA/ctDNA) and circulating cell-free (tumor) RNA (cfRNA/ctRNA) shows great promise in determining the molecular profile of cancer patients. Optimization of the workflow is necessary to achieve consistent and reproducible results. In this study, we compared five centrifugation protocols for the optimal yield of both cfDNA/ctDNA and cfRNA/ctRNA. These protocols varied in centrifugation speed, ambient temperature, time, and number of centrifugation steps. Samples from 33 participants were collected in either BD Vacutainer K2EDTA (EDTA) tubes or cell-free DNA BCT® (Streck) tubes. cfDNA concentration and fragment size, and cfRNA concentration were quantitated in all samples by digital droplet PCR (ddPCR) and quantitative PCR (qPCR). The KRAS-mutated ctDNA and ctRNA fraction was determined via ddPCR. In EDTA tubes, the protocol generating both plasma and platelets was found to produce high quality cfDNA and cfRNA concentrations. Two-step, high-speed centrifugation protocols were associated with high cfDNA but low cfRNA concentrations. High cfRNA concentrations were generated by a one-step, low-speed protocol. However, this coincided with a high amount of genomic DNA (gDNA) contamination. In Streck tubes, two-step, high-speed centrifugation protocols also generated good quality, high cfDNA concentration. However, these tubes are not compatible with cfRNA analysis.
Cx43 Expression Correlates with Breast Cancer Metastasis in MDA-MB-231 Cells In Vitro, In a Mouse Xenograft Model and in Human Breast Cancer Tissues
Authors: Kazan Jalal M. , El-Saghir Jamal , Saliba Jessica , Shaito Abdullah , Jalaleddine Nour , El-Hajjar Layal , Al-Ghadban Sara , Yehia Lamis , Zibara Kazem , El-Sabban Marwan
Published: April 2019

Connexins regulate multiple cellular functions and are considered tumor suppressors. Connexin43 (Cx43) is frequently down-regulated in breast tumors. However, Cx43 regulation during cancer onset and metastasis is complex and context-dependent. We investigated the effect of Cx43 over-expression or knock-down on the metastatic potential of MDA-MB-231 breast cancer cells in vitro and in vivo and in human breast cancer tissues. MDA-MB-231 cells over-expressing (Cx43D) or down-regulating Cx43 (shCx43) were generated and used in proliferation, migration, and invasion assays. The regulation of genes/proteins implicated in progression, invasion and metastasis was assessed in vitro and in immune-compromized mice injected with MDA-MB-231, Cx43D or shCx43 cells. Primary tumor onset/growth, metastasis and overall survival of these animals was monitored and evaluated. In addition, Cx43 expression in human breast carcinoma samples was assessed by qPCR. Cx43 over-expression increased protein levels of epithelial markers E-cadherin and zonula occludens 1 expression and resulted in the sequestration of β-catenin at the cell membrane, while Cx43 knock-down induced protein expression of the mesenchymal marker N-cadherin and an increased invasive potential of shCx43 cells. In vivo, in mice xenografted with breast cancer cells, Cx43 over-expression decreased tumor volume, attenuated cell metastasis to lungs and liver and increased overall mice survival. Importantly, the expression of Cx43 in triple negative human breast cancer tissues is also down-regulated. Collectively, Cx43 over-expression induced an epithelial-like phenotype in MDA-MB-231 cells and suppressed tumor growth and metastasis to secondary organs in vivo. In contrast, Cx43 knock-down in MDA-MB-231 cells induced a mesenchymal phenotype with increased cell invasion leading to an enhanced metastatic phenotype. These data provide evidence for a pivotal role of Cx43 in breast cancer metastasis and support the potential targeting of connexins in breast cancer therapy.
Chondroitin Sulfate-Degrading Enzymes as Tools for the Development of New Pharmaceuticals
Authors: Benito-Arenas Raúl , Zárate Sandra G. , Revuelta Julia , Bastida Agatha
Published: April 2019

Chondroitin sulfates are linear anionic sulfated polysaccharides found in biological tissues, mainly within the extracellular matrix, which are degraded and altered by specific lyases depending on specific time points. These polysaccharides have recently acquired relevance in the pharmaceutical industry due to their interesting therapeutic applications. As a consequence, chondroitin sulfate (CS) lyases have been widely investigated as tools for the development of new pharmaceuticals based on these polysaccharides. This review focuses on the major breakthrough represented by chondroitin sulfate-degrading enzymes and their structures and mechanisms of function in addition to their major applications.
Contribution of High Mechanical Fatigue to Gas Turbine Blade Lifetime during Steady-State Operation
Authors: Chang Sung Yong , Oh Ki-Yong
Published: April 2019

In this study, the contribution of high thermomechanical fatigue to the gas turbine lifetime during a steady-state operation is evaluated for the first time. An evolution of the roughness on the surface between the thermal barrier coating and bond coating is addressed to elucidate the correlation between operating conditions and the degradation of a gas turbine. Specifically, three factors affecting coating failure are characterized, namely isothermal operation, low-cycle fatigue, and high thermomechanical fatigue, using laboratory experiments and actual service-exposed blades in a power plant. The results indicate that, although isothermal heat exposure during a steady-state operation contributes to creep, it does not contribute to failure caused by coating fatigue. Low-cycle fatigue during a transient operation cannot fully describe the evolution of the roughness between the thermal barrier coating and the bond coating of the gas turbine. High thermomechanical fatigue during a steady-state operation plays a critical role in coating failure because the temperature of hot gas pass components fluctuates up to 140 °C at high operating temperatures. Hence, high thermomechanical fatigue must be accounted for to accurately predict the remaining useful lifetime of a gas turbine because the current method of predicting the remaining useful lifetime only accounts for creep during a steady-state operation and for low-cycle fatigue during a transient operation.
Structure and Properties of Protective Coatings Deposited by Pulsed Cathodic Arc Evaporation in Ar, N2, and C2H4 Environments using the TiC–NiCr–Eu2O3 Cathode
Authors: Kiryukhantsev-Korneev Philipp , Sytchenko Alina , Sheveyko Alexander , Vorotilo Stepan
Published: April 2019

Coatings were deposited by pulsed cathodic arc evaporation (PCAE) of a TiC–NiCr–Eu2O3 cathode fabricated by the powder metallurgy method. The deposition was carried out in different gas media, including Ar, N2, and C2H4. The structure, elemental, and phase compositions of coatings were studied by scanning electron microscopy (SEM), X-ray diffraction (XRD), energy-dispersive spectroscopy (EDS), Raman spectroscopy, and glow discharge optical emission spectroscopy (GDOES). Coatings were tested in terms of their hardness, elastic modulus, elastic recovery, friction coefficient, and wear and corrosion resistance. The obtained results demonstrated that the coatings deposited in Ar possessed higher hardness up to 20 GPa and an elastic recovery of 92%. Coatings produced using C2H4 showed the minimum friction coefficient (0.35 ± 0.01). The use of nitrogen as a gas medium led to the formation of coatings with the best corrosion resistance in sulfuric acid. Coatings formed in N2 had a free corrosion potential of +0.28 V and a corrosion current density of 0.012 µA/cm2.
Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution
Authors: Susek Karolina , Bielski Wojciech , Czyż Katarzyna B. , Hasterok Robert , Jackson Scott A. , Wolko Bogdan , Naganowska Barbara
Published: April 2019

Plant genome evolution can be very complex and challenging to describe, even within a genus. Mechanisms that underlie genome variation are complex and can include whole-genome duplications, gene duplication and/or loss, and, importantly, multiple chromosomal rearrangements. Lupins (Lupinus) diverged from other legumes approximately 60 mya. In contrast to New World lupins, Old World lupins show high variability not only for chromosome numbers (2n = 32–52), but also for the basic chromosome number (x = 5–9, 13) and genome size. The evolutionary basis that underlies the karyotype evolution in lupins remains unknown, as it has so far been impossible to identify individual chromosomes. To shed light on chromosome changes and evolution, we used comparative chromosome mapping among 11 Old World lupins, with Lupinus angustifolius as the reference species. We applied set of L. angustifolius-derived bacterial artificial chromosome clones for fluorescence in situ hybridization. We demonstrate that chromosome variations in the species analyzed might have arisen from multiple changes in chromosome structure and number. We hypothesize about lupin karyotype evolution through polyploidy and subsequent aneuploidy. Additionally, we have established a cytogenomic map of L. angustifolius along with chromosome markers that can be used for related species to further improve comparative studies of crops and wild lupins.
Satellite SAR Data-based Sea Ice Classification: An Overview
Authors: Zakhvatkina Natalia , Smirnov Vladimir , Bychkova Irina
Published: April 2019

A review of the main approaches developed for sea ice classification using satellite imagery is presented. Satellite data are the main and very often only information source for sea ice classification and charting in the remote arctic regions. The main techniques used for ice classification and ice charting in several national ice services are considered. Advantages and disadvantages of various SAR data-based methods for ice classification are analyzed. It is shown that an increase of SAR technical abilities contributes to the enhancement of sea ice classification reliability. The possible further development of satellite data-based methods for ice classification is discussed.
Titanian Clinohumite-Bearing Peridotite from the Ulamertoq Ultramafic Body in the 3.0 Ga Akia Terrane of Southern West Greenland
Authors: Nishio Ikuya , Morishita Tomoaki , Szilas Kristofer , Pearson Graham , Tani Ken-Ichiro , Tamura Akihiro , Harigane Yumiko , Guotana Juan Miguel
Published: April 2019

A titanian clinohumite-bearing dunite was recently found in the Ulamertoq ultramafic body within the 3.0 Ga Akia Terrane of southern West Greenland. Titanian clinohumite occurs as disseminated and discrete grains. Titanian clinohumite contains relatively high amounts of fluorine, reaching up to 2.4 wt.%. The high-Fo content of olivine (Fo93) coupled with low Cr/(Cr + Al) ratio of orthopyroxene implies that the dunite host is not of residual origin after melt extraction by partial melting of the primitive mantle. Olivine grains are classified into two types based on abundances of opaque mineral inclusions: (1) dusty inclusion-rich and (2) clear inclusion-free olivines. Opaque inclusions in coarse-grained olivines are mainly magnetite. Small amounts of ilmenite are also present around titanian clinohumite grains. The observed mineral association indicates partial replacement of titanian clinohumite to ilmenite (+magnetite) and olivine following the reaction: titanian clinohumite = ilmenite + olivine + hydrous fluid. The coexistence of F-bearing titanian clinohumite, olivine, and chromian chlorite indicates equilibration at around 800–900 °C under garnet-free conditions (<2 GPa). Petrological and mineralogical characteristics of the studied titanian clinohumite-bearing dunite are comparable to deserpentinized peridotites derived from former serpentinites. This study demonstrates the importance of considering the effects of hydration/dehydration processes for the origin of ultramafic bodies found in polymetamorphic Archaean terranes.
The Evaluation of the Heavy Metal Leaching Behavior of MSWI-FA Added Alkali-Activated Materials Bricks by Using Different Leaching Test Methods
Authors: Xu Peng , Zhao Qingliang , Qiu Wei , Xue Yan
Published: April 2019

Alkali-activated materials (AAMs) not only have the potential to replace cement applications in architecture and civil engineering, but also have an excellent effect on the stabilization solidification of hazardous industrial wastes. This study used two types of municipal solid waste incineration fly ash (MSWI-FA)—grate firing fly ash (GFFA) and fluidized bed fly ash (FBFA)—as AAMs brick raw materials. It is discovered from this study that AAMs bricks with different weight ratios of GFFA and FBFA can both meet the required standard of GB21144-2007 (Solid concrete brick). From the results obtained from the four leaching tests, the equilibrium pH of the leachate varies, resulting in significant differences in the leaching of heavy metals in Raw GFFA, Raw FBFA, and AAMs bricks with GFFA and FBFA. The AAMs brick with the addition of GFFA and FBFA has an alkali activation system to encapsulate heavy metals. By comparing the results obtained from the CEN/TS 14429 leaching behavior test and the four batch leaching tests, it was found that the most influential factors for the heavy metal leaching concentration are whether the heavy metal has been solidified/stabilized in the samples. GFFA and FBFA tend to have consistent characteristics after being activated by alkali to form AAMs bricks. This can be confirmed by the acid neutralization ability concentrated on a specific pH range. The results obtained from CEN/TS14429 verified that the AAMs bricks with the addition of GFFA and FBFA have excellent environmental compatibility and that it provides a comprehensive evaluation on the environmental compatibility of the test materials and products. This demonstrated that the MSWI-FA is suitable for used as alkali-activated materials and its products have the potential to be commercially used in the future.
Overexpression of Long-Chain Acyl-CoA Synthetase 5 Increases Fatty Acid Oxidation and Free Radical Formation While Attenuating Insulin Signaling in Primary Human Skeletal Myotubes
Authors: Kwak Hyo-Bum , Woodlief Tracey L. , Green Thomas D. , Cox Julie H. , Hickner Robert C. , Neufer P. Darrell , Cortright Ronald N.
Published: April 2019

In rodent skeletal muscle, acyl-coenzyme A (CoA) synthetase 5 (ACSL-5) is suggested to localize to the mitochondria but its precise function in human skeletal muscle is unknown. The purpose of these studies was to define the role of ACSL-5 in mitochondrial fatty acid metabolism and the potential effects on insulin action in human skeletal muscle cells (HSKMC). Primary myoblasts isolated from vastus lateralis (obese women (body mass index (BMI) = 34.7 ± 3.1 kg/m2)) were transfected with ACSL-5 plasmid DNA or green fluorescent protein (GFP) vector (control), differentiated into myotubes, and harvested (7 days). HSKMC were assayed for complete and incomplete fatty acid oxidation ([1-14C] palmitate) or permeabilized to determine mitochondrial respiratory capacity (basal (non-ADP stimulated state 4), maximal uncoupled (carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP)-linked) respiration, and free radical (superoxide) emitting potential). Protein levels of ACSL-5 were 2-fold higher in ACSL-5 overexpressed HSKMC. Both complete and incomplete fatty acid oxidation increased by 2-fold (p < 0.05). In permeabilized HSKMC, ACSL-5 overexpression significantly increased basal and maximal uncoupled respiration (p < 0.05). Unexpectedly, however, elevated ACSL-5 expression increased mitochondrial superoxide production (+30%), which was associated with a significant reduction (p < 0.05) in insulin-stimulated p-Akt and p-AS160 protein levels. We concluded that ACSL-5 in human skeletal muscle functions to increase mitochondrial fatty acid oxidation, but contrary to conventional wisdom, is associated with increased free radical production and reduced insulin signaling.
The Relationship between Body Fat Percentage and Some Anthropometric and Physical Fitness Characteristics in Pre- and Peripubertal Boys
Authors: Szmodis Márta , Szmodis Iván , Farkas Anna , Mészáros Zsófia , Mészáros János , Kemper Han C.G.
Published: April 2019

The main aim of this study was to compare anthropometric and physical fitness indicators of boys of the same chronical age but with different fat percentages. Subjects were Hungarian boys aged 9–13 years (N = 6919). Anthropometry was measured according the guidelines of the International Biological Program. Relative body fat was estimated by Drinkwater–Ross’s method (1980); Conrad’s growth type of physique was also estimated (1963). Physical fitness was tested with 30 m dash (s), standing long jump (cm), fistball throw (m), and 1200 m run (s). Subjects of each cohort were grouped into seven subgroups with fat percentage ranges of 4%. Differences between subgroups were tested by one-way ANOVA. In the case of a significant F-test, Tukey’s post-hoc tests were used. The level of effective random error was set at 5% in all significance tests (p < 0.05). Except for the three groups with low fat percentages, values of body weight, stature, body mass index, and plastic and metric indexes were significantly higher; results of 30 m, 1200 m running, and standing long jump were worse in all groups with higher fat percentages. An interesting finding of the current study is that body fat percentage also influenced the physical fitness of non-overweight and obese children as well when using merely the 4% ranges in grouping by fatness. The lower the fat the better the physical fitness was in this sample of pre- and peripubertal boys.
Do Protected Areas Contribute to Health and Well-Being? A Cross-Cultural Comparison
Authors: Jiricka-Pürrer Alexandra , Tadini Valeria , Salak Boris , Taczanowska Karolina , Tucki Andrzej , Senes Giulio
Published: April 2019

Due to their valuable landscape and natural characteristics, protected areas (PAs) distinguish themselves from other green spaces. Studies that survey individuals on the perceived capacity of PAs to provide health benefits are very limited. However, the importance of PAs for societal health could emphasize the necessity to preserve them. In addition, studies of cross-country comparisons of nature-based activities show strong cultural differences with regard to the importance of wild nature and landscape preferences. Cross-country comparisons of the perception of PAs as a resource for personal well-being and health are lacking. An extensive survey with face-to-face questionnaires was conducted in PAs in Poland, Austria, and Italy with an overall sample size of 1390 people. It examined both emotional and physical personal well-being in the context of a stay in a PA. In general, the results showed that between the three countries, different perceptions of the capacity of PAs to contribute to personal health and well-being exist. Both diverse emotional and physical benefits were associated with a stay in a PA. Moreover, respondents from the three countries assessed the perceived capacity of a PA to reduce negative physical symptoms, such as muscular pain or tension, and negative emotional symptoms, such as depression or stress.