Effect of bee pollen extract on lead-induced toxicity in rat testis
Authors: Bouazza Sofiane , Demmouche Abbassia , Toumi-Benali Fawzia , Zouba Mouad , Bahri Mohamed Rabie , Agher Nawel , Merakchi Nacera , Ahmar Mama El 
Published: August 2021

<!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:-536870145 1107305727 0 0 415 0;} @font-face {font-family:"Calibri Light"; panose-1:2 15 3 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 415 0;} @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:-536870145 1073786111 1 0 415 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:6.0pt; margin-left:0cm; text-align:justify; text-indent:21.25pt; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri Light"; mso-ascii-font-family:"Calibri Light"; mso-ascii-theme-font:major-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:"Calibri Light"; mso-hansi-theme-font:major-latin; mso-bidi-font-family:"Calibri Light"; mso-bidi-theme-font:major-latin; mso-ansi-language:EN-GB;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:11.0pt; mso-ansi-font-size:11.0pt; mso-bidi-font-size:11.0pt; font-family:Calibri; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-ansi-language:FR;} .MsoPapDefault {mso-style-type:export-only; margin-bottom:8.0pt; line-height:107%;} @page WordSection1 {size:612.0pt 792.0pt; margin:72.0pt 72.0pt 72.0pt 72.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.WordSection1 {page:WordSection1;} --> Collected by honeybees, Bee pollen is a flower pollen gathered from selected flower species by special pollen traps, therapeutic qualities and reproductive performance of bee pollen are given from biologically active substances. Lead is a heavy metal, which induce reproductive intoxication, comes in the high levels from human activities.The aim of the present study was to evaluate the impact of bee pollen hydro-ethanolic extract on adult Wistar rat testis exposed to lead acetate sub-acute intoxication (15 days), highlighted using histological technics in situ. The biochemical and biometric measurement were conducted to verify any eventual toxicity of hydro-ethanolic extract in vivo. Animals were divided into four equal groups: first control group was treated orally with distillated water (1 ml) by gavage. The second group was administered 100 mg/kg/bw/day of a hydro-ethanolic extract of bee pollen, the third group was administered 30 mg/kg/bw/day lead acetate, the fourth group was given the both treatments in the same levels. The results showed that the relative organs weight indicates that only left kidney (p=0.001) and spleen (p=0.001) were attenuated without a considerable difference of the liver (p=0.07). the same was shown for relative weight of sexual organs. In addition, no significant difference, was marked, in serum biochemical parameters except for: Blood urea nitrogen, Alanine aminotransferase, Total bilirubin and Direct bilirubin. furthermore, ameliorative effect was shown after lead-induced alteration after histological stain of seminiferous tubules, indicating low lumen of seminiferous tubules with presence of spermatogonia and wide height of seminiferous epithelium. Biological activities of bee pollen extract, alleviate the lead induced toxicity on rat testis, marked by a complete spermatogenesis and prevention of Sertoli cells destruction.