bluuebunny/crossref_metadata_2025 huggingface dataset:
Origin of Extremely Intense Southward Component of Magnetic Field (Bs) in ICMEs
Authors: Shen Chenglong , Chi Yutian , Xu Mengjiao , Wang Yuming
Published: December 2021

The intensity of the southward component of the magnetic field (Bs) carried by Interplanetary Coronal Mass Ejections (ICMEs) is one of the most critical parameters in causing extreme space weather events, such as intense geomagnetic storms. In this work, we investigate three typical ICME events with extremely intense Bs in detail and present a statistical analysis of the origins of intense Bs in different types of ICMEs based on the ICME catalogue from 1995 to 2020. According to the in-situ characteristics, the ICME events with extremely high Bs are classified into three types: isolated ICMEs, multiple ICMEs, and shock-ICME interaction events with shocks inside ICMEs or shocks passing through ICMEs. By analyzing all ICME events with Bs ≥ 10nT and Bs ≥ 20nT, we find that 39.6% of Bs,mean ≥ 10nT events and 50% of Bs,mean ≥ 20nT events are associated with shock-ICME events. Approximately 35.7% of shock-ICME events have Bs,mean ≥ 10nT, which is much higher than the other two types (isoloted ICMEs: 7.2% and multiple ICMEs: 12.1%). Those results confirm that the ICMEs interaction events are more likely to carry extreme intense Bs and cause intense geomagntic storms. Only based on the in-situ observations at Earth, some interaction ICME events, such as shock-ICME interaction events with shocks passing through the preceding ICME or ICME cannibalism, could be classified as isolated ICME events. This may lead to an overestimate of the probability of ICME carrying extremely intense Bs. To further investigate such events, direct and multi-point observations of the CME propagation in the inner heliosphere from the Solar Ring Mission could be crucial in the future.
Antiphospholipid Antibodies From Women With Pregnancy Morbidity and Vascular Thrombosis Induce Endothelial Mitochondrial Dysfunction, mTOR Activation, and Autophagy
Authors: Rodríguez Carlos M. , Velásquez-Berrío Manuela , Rúa Carolina , Viana Marta , Abrahams Vikki M. , Cadavid Angela P. , Alvarez Angela M.
Published: December 2021

Antiphospholipid syndrome (APS) is an autoimmune disease characterized by thrombosis and pregnancy morbidity (PM) obstetric events together with persistent high titers of circulating antiphospholipid antibodies (aPL). Several mechanisms that explain the development of thrombosis and PM in APS include the association of aPL with alterations in the coagulation cascade and inflammatory events. Other mechanisms disturbing cellular homeostases, such as mitochondrial dysfunction, autophagy, and cell proliferation, have been described in other autoimmune diseases. Therefore, the objective of this study was to investigate the impact of aPL from different patient populations on endothelial cell mitochondrial function, activation of the mammalian target of rapamycin (mTOR) and autophagy pathways, and cellular growth. Using an in vitro model, human umbilical vein endothelial cells (HUVECs) were treated with polyclonal immunoglobulin G (IgG) purified from the serum of women with both PM and vascular thrombosis (PM/VT), with VT only (VT), or with PM and non-criteria aPL (seronegative-obstetric APS, SN-OAPS). We included IgG from women with PM without aPL (PM/aPL-) and healthy women with previous uncomplicated pregnancies (normal human serum, NHS) as control groups. Mitochondrial function, mTOR activation, autophagy, and cell proliferation were evaluated by Western blotting, flow cytometry, and functional assays. IgG from women with PM/VT increased HUVEC mitochondrial hyperpolarization and activation of the mTOR and autophagic pathways, while IgG from patients with VT induced endothelial autophagy and cell proliferation in the absence of elevated mTOR activity or mitochondrial dysfunction. IgG from the SN-OAPS patient group had no effect on any of these HUVEC responses. In conclusion, aPL from women with PM and vascular events induce cellular stress evidenced by mitochondrial hyperpolarization and increased activation of the mTOR and autophagic pathways which may play a role in the pathogenesis of obstetric APS.
Water Use, Leaf Cooling and Carbon Assimilation Efficiency of Heat Resistant Common Beans Evaluated in Western Amazonia
Authors: Suárez Juan Carlos , Urban Milan O. , Contreras Amara Tatiana , Noriega Jhon Eduar , Deva Chetan , Beebe Stephen E. , Polanía José A. , Casanoves Fernando , Rao Idupulapati M.
Published: December 2021

In our study, we analyzed 30years of climatological data revealing the bean production risks for Western Amazonia. Climatological profiling showed high daytime and nighttime temperatures combined with high relative humidity and low vapor pressure deficit. Our understanding of the target environment allows us to select trait combinations for reaching higher yields in Amazonian acid soils. Our research was conducted using 64 bean lines with different genetic backgrounds. In high temperatures, we identified three water use efficiency typologies in beans based on detailed data analysis on gasometric exchange. Profligate water spenders and not water conservative accessions showed leaf cooling, and effective photosynthate partitioning to seeds, and these attributes were found to be related to higher photosynthetic efficiency. Thus, water spenders and not savers were recognized as heat resistant in acid soil conditions in Western Amazonia. Genotypes such as BFS 10, SEN 52, SER 323, different SEFs (SEF 73, SEF 10, SEF 40, SEF 70), SCR 56, SMR 173, and SMN 99 presented less negative effects of heat stress on yield. These genotypes could be suitable as parental lines for improving dry seed production. The improved knowledge on water-use efficiency typologies can be used for bean crop improvement efforts as well as further studies aimed at a better understanding of the intrinsic mechanisms of heat resistance in legumes.
Characterization of Metal Tolerance Proteins and Functional Analysis of GmMTP8.1 Involved in Manganese Tolerance in Soybean
Authors: Li Jifu , Dong Rongshu , Jia Yidan , Huang Jie , Zou Xiaoyan , An Na , Song Jianling , Chen Zhijian
Published: December 2021

Manganese is an essential micronutrient for plant growth but can be toxic to plants when it reaches excessive levels. Although metal tolerance proteins (MTPs), which belong to the cation diffusion facilitator (CDF) family, have been demonstrated to play critical roles in manganese (Mn) tolerance in plants, the characteristics and functions of GmMTP members in the response of soybean (Glycine max) to Mn toxicity have not been documented. In this study, growth inhibition was observed in soybean plants that were exposed to a toxic level of Mn in hydroponics, as reflected by the generation of brown spots, and decreased leaf chlorophyll concentration and plant fresh weight. Subsequent genome-wide analysis resulted in the identification of a total of 14 GmMTP genes in the soybean genome. Among these GmMTPs, 9 and 12 were found to be regulated by excess Mn in leaves and roots, respectively. Furthermore, the function of GmMTP8.1, a Mn-CDF homologue of ShMTP8 identified in the legume Stylosanthes hamata that is involved in Mn detoxification, was characterized. Subcellular localization analysis showed that GmMTP8.1 was localized to the endoplasmic reticulum (ER). Heterologous expression of GmMTP8.1 led to the restoration of growth of the Mn-hypersensitive yeast (Saccharomyces cerevisiae) mutant Δpmr1, which is made defective in Mn transport into the Golgi apparatus by P-type Ca/Mn-ATPase. Furthermore, GmMTP8.1 overexpression conferred tolerance to the toxic level of Mn in Arabidopsis (Arabidopsis thaliana). Under excess Mn conditions, concentrations of Mn in shoots but not roots were decreased in transgenic Arabidopsis, overexpressing GmMTP8.1 compared to the wild type. The overexpression of GmMTP8.1 also led to the upregulation of several transporter genes responsible for Mn efflux and sequestration in Arabidopsis, such as AtMTP8/11. Taken together, these results suggest that GmMTP8.1 is an ER-localized Mn transporter contributing to confer Mn tolerance by stimulating the export of Mn out of leaf cells and increasing the sequestration of Mn into intracellular compartments.
The Genomic Architecture of Competitive Response of Arabidopsis thaliana Is Highly Flexible Among Plurispecific Neighborhoods
Authors: Libourel Cyril , Baron Etienne , Lenglet Juliana , Amsellem Laurent , Roby Dominique , Roux Fabrice
Published: December 2021

Plants are daily challenged by multiple abiotic and biotic stresses. A major biotic constraint corresponds to competition with other plant species. Although plants simultaneously interact with multiple neighboring species throughout their life cycle, there is still very limited information about the genetics of the competitive response in the context of plurispecific interactions. Using a local mapping population of Arabidopsis thaliana, we set up a genome wide association study (GWAS) to estimate the extent of genetic variation of competitive response in 12 plant species assemblages, based on three competitor species (Poa annua, Stellaria media, and Veronica arvensis). Based on five phenotypic traits, we detected strong crossing reaction norms not only between the three bispecific neighborhoods but also among the plurispecific neighborhoods. The genetic architecture of competitive response was highly dependent on the identity and the relative abundance of the neighboring species. In addition, most of the enriched biological processes underlying competitive responses largely differ among neighborhoods. While the RNA related processes might confer a broad range response toolkit for multiple traits in diverse neighborhoods, some processes, such as signaling and transport, might play a specific role in particular assemblages. Altogether, our results suggest that plants can integrate and respond to different species assemblages depending on the identity and number of each neighboring species, through a large range of candidate genes associated with diverse and unexpected processes leading to developmental and stress responses.
Climate and Competitive Status Modulate the Variation in Secondary Metabolites More in Leaves Than in Fine Roots of Betula pendula
Authors: Tullus Arvo , Rusalepp Linda , Lutter Reimo , Rosenvald Katrin , Kaasik Ants , Rytter Lars , Kontunen-Soppela Sari , Oksanen Elina
Published: December 2021

Plant secondary metabolites have many important functions; they also determine the productivity and resilience of trees under climate change. The effects of environmental factors on secondary metabolites are much better understood in above-ground than in below-ground part of the tree. Competition is a crucial biotic stress factor, but little is known about the interaction effect of climate and competition on the secondary chemistry of trees. Moreover, competition effect is usually overlooked when analyzing the sources of variation in the secondary chemistry. Our aim was to clarify the effects of competitive status, within-crown light environment, and climate on the secondary chemistry of silver birch (Betula pendula Roth). We sampled leaves (from upper and lower crown) and fine roots from competitively dominant and suppressed B. pendula trees in plantations along a latitudinal gradient (56–67° N) in Fennoscandia, with mean annual temperature (MAT) range: −1 to 8°C. Secondary metabolites in leaves (SML) and fine roots (SMFR) were determined with an HPLC-qTOF mass spectrometer. We found that SML content increased significantly with MAT. The effect of competitive stress on SML strengthened in colder climates (MAT<4°C). Competition and shade initiated a few similar responses in SML. SMFR varied less with MAT. Suppressed trees allocated relatively more resources to SML in warmer climates and to SMFR in colder ones. Our study revealed that the content and profile of secondary metabolites (mostly phenolic defense compounds and growth regulators) in leaves of B. pendula varied with climate and reflected the trees’ defense requirements against herbivory, exposure to irradiance, and competitive status (resource supply). The metabolic profile of fine roots reflected, besides defense requirements, also different below-ground competition strategies in warmer and colder climates. An increase in carbon assimilation to secondary compounds can be expected at northern latitudes due to climate change.
Regeneration and Endogenous Phytohormone Responses to High-Temperature Stress Drive Recruitment Success in Hemiepiphytic Fig Species
Authors: Fang Chuangwei , Chen Huayang , Castillo-Díaz Diana , Wen Bin , Cao Kun-Fang , Goodale Uromi Manage
Published: December 2021

Exposure to high-temperature stress (HTS) during early regeneration in plants can profoundly shape seed germination, seedling growth, and development, thereby providing stress resilience. In this study, we assessed how the timing of HTS, which was implemented as 8 h in 40°C, could affect the early regeneration stages and phytohormone concentration of four hemiepiphytic (Hs) and four non-hemiepiphytic (NHs) Ficus species. Their seed germination, seedling emergence, and seedling survival probabilities and the concentrations of three endogenous phytohormones, abscisic acid (ABA), indole-3-acetic acid (IAA), and salicylic acid (SA) were assessed after HTS imposed during imbibition, germination, and emergence. In both groups, seeds were more sensitive to HTS in the early regeneration process; stress experienced during imbibition affected emergence and survival, and stress experienced during germination affected subsequent emergence. There was no effect from HTS when received after emergence. Survival was highest in hemiepiphytes regardless of the HTS treatment. The phytohormones showed growth form- and regeneration stage-specific responses to HTS. Due to the HTS treatment, both SA and ABA levels decreased in non-hemiepiphytes during imbibition and germination; during germination, IAA increased in hemiepiphytes but was reduced in non-hemiepiphytes. Due to the HTS treatment experienced during emergence ABA and IAA concentrations were greater for hemiepiphytes but an opposite effect was seen in the two growth forms for the SA concentration. Our study showed that the two growth forms have different strategies for regulating their growth and development in the early regeneration stages in order to respond to HTS. The ability to respond to HTS is an ecologically important functional trait that allows plant species to appropriately time their seed germination and seedling development. Flexibility in modulating species regeneration in response to HTS in these subtropical and tropical Ficus species could provide greater community resilience under climate change.
Complete Chloroplast Genome Sequence of Erigeron breviscapus and Characterization of Chloroplast Regulatory Elements
Authors: Yu Yifan , Ouyang Zhen , Guo Juan , Zeng Wen , Zhao Yujun , Huang Luqi
Published: December 2021

Erigeron breviscapus is a famous medicinal plant. However, the limited chloroplast genome information of E. breviscapus, especially for the chloroplast DNA sequence resources, has hindered the study of E. breviscapus chloroplast genome transformation. Here, the complete chloroplast (cp) genome of E. breviscapus was reported. This genome was 152,164bp in length, included 37.2% GC content and was structurally arranged into two 24,699bp inverted repeats (IRs) and two single-copy areas. The sizes of the large single-copy region and the small single-copy region were 84,657 and 18,109bp, respectively. The E. breviscapus cp genome consisted of 127 coding genes, including 83 protein coding genes, 36 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes. For those genes, 95 genes were single copy genes and 16 genes were duplicated in two inverted regions with seven tRNAs, four rRNAs, and five protein coding genes. Then, genomic DNA of E. breviscapus was used as a template, and the endogenous 5' and 3' flanking sequences of the trnI gene and trnA gene were selected as homologous recombinant fragments in vector construction and cloned through PCR. The endogenous 5' flanking sequences of the psbA gene and rrn16S gene, the endogenous 3' flanking sequences of the psbA gene, rbcL gene, and rps16 gene and one sequence element from the psbN-psbH chloroplast operon were cloned, and certain chloroplast regulatory elements were identified. Two homologous recombination fragments and all of these elements were constructed into the cloning vector pBluescript SK (+) to yield a series of chloroplast expression vectors, which harbored the reporter gene EGFP and the selectable marker aadA gene. After identification, the chloroplast expression vectors were transformed into Escherichia coli and the function of predicted regulatory elements was confirmed by a spectinomycin resistance test and fluorescence intensity measurement. The results indicated that aadA gene and EGFP gene were efficiently expressed under the regulation of predicted regulatory elements and the chloroplast expression vector had been successfully constructed, thereby providing a solid foundation for establishing subsequent E. breviscapus chloroplast transformation system and genetic improvement of E. breviscapus.
Flavonoids Modulate the Accumulation of Toxins From Aspergillus flavus in Maize Kernels
Authors: Castano-Duque Lina , Gilbert Matthew K. , Mack Brian M. , Lebar Matthew D. , Carter-Wientjes Carol H. , Sickler Christine M. , Cary Jeffrey W. , Rajasekaran Kanniah
Published: December 2021

Aspergillus flavus is an opportunistic fungal pathogen capable of producing aflatoxins, potent carcinogenic toxins that accumulate in maize kernels after infection. To better understand the molecular mechanisms of maize resistance to A. flavus growth and aflatoxin accumulation, we performed a high-throughput transcriptomic study in situ using maize kernels infected with A. flavus strain 3357. Three maize lines were evaluated: aflatoxin-contamination resistant line TZAR102, semi-resistant MI82, and susceptible line Va35. A modified genotype-environment association method (GEA) used to detect loci under selection via redundancy analysis (RDA) was used with the transcriptomic data to detect genes significantly influenced by maize line, fungal treatment, and duration of infection. Gene ontology enrichment analysis of genes highly expressed in infected kernels identified molecular pathways associated with defense responses to fungi and other microbes such as production of pathogenesis-related (PR) proteins and lipid bilayer formation. To further identify novel genes of interest, we incorporated genomic and phenotypic field data from a genome wide association analysis with gene expression data, allowing us to detect significantly expressed quantitative trait loci (eQTL). These results identified significant association between flavonoid biosynthetic pathway genes and infection by A. flavus. In planta fungal infections showed that the resistant line, TZAR102, has a higher fold increase of the metabolites naringenin and luteolin than the susceptible line, Va35, when comparing untreated and fungal infected plants. These results suggest flavonoids contribute to plant resistance mechanisms against aflatoxin contamination through modulation of toxin accumulation in maize kernels.
Extending the Cultivation Area of Pecan (Carya illinoinensis) Toward the South in Southeastern Subtropical China May Cause Increased Cold Damage
Authors: Zheng Jinbin , Hänninen Heikki , Lin Jianhong , Shen Sitian , Zhang Rui
Published: December 2021

Pecan (Carya illinoinensis) is an important nut tree species in its native areas in temperate and subtropical North America, and as an introduced crop in subtropical southeastern China as well. We used process-based modeling to assess the effects of climatic warming in southeastern China on the leaf-out phenology of pecan seedlings and the subsequent risk of “false springs,” i.e., damage caused by low temperatures occurring as a result of prematurely leafing out. In order to maximize the biological realism of the model used in scenario simulations, we developed the model on the basis of experiments explicitly designed for determining the responses modeled. The model showed reasonable internal accuracy when calibrated against leaf-out observations in a whole-tree chamber (WTC) experiment with nine different natural-like fluctuating temperature treatments. The model was used to project the timing of leaf-out in the period 2022–2099 under the warming scenarios RCP4.5 and RCP8.5 in southeastern China. Two locations in the main pecan cultivation area in the northern subtropical zone and one location south of the main cultivation area were addressed. Generally, an advancing trend of leaf-out was projected for all the three locations under both warming scenarios, but in the southern location, a delay was projected under RCP8.5 in many years during the first decades of the 21st century. In the two northern locations, cold damage caused by false springs was projected to occur once in 15–26 years at most, suggesting that pecan cultivation can be continued relatively safely in these two locations. Paradoxically, more frequent cold damage was projected for the southern location than for the two northern locations. The results for the southern location also differed from those for the northern locations in that more frequent cold damage was projected under the RCP4.5 warming scenario (once in 6 years) than under the RCP8.5 scenario (once in 11 years) in the southern location. Due to the uncertainties of the model applied, our conclusions need to be re-examined in an additional experimental study and further model development based on it; but on the basis of our present results, we do not recommend starting large-scale pecan cultivation in locations south of the present main pecan cultivation area in southeastern subtropical China.
Plant Functional Groups Dominate Responses of Plant Adaptive Strategies to Urbanization
Authors: Xiao Yihua , Liu Shirong , Zhang Manyun , Tong Fuchun , Xu Zhihong , Ford Rebecca , Zhang Tianlin , Shi Xin , Wu Zhongmin , Luo Tushou
Published: December 2021

Urbanization causes alteration in atmospheric, soil, and hydrological factors and substantially affects a range of morphological and physiological plant traits. Correspondingly, plants might adopt different strategies to adapt to urbanization promotion or pressure. Understanding of plant traits responding to urbanization will reveal the capacity of plant adaptation and optimize the choice of plant species in urbanization green. In this study, four different functional groups (herbs, shrubs, subcanopies, and canopies, eight plant species totally) located in urban, suburban, and rural areas were selected and eight replicated plants were selected for each species at each site. Their physiological and photosynthetic properties and heavy metal concentrations were quantified to reveal plant adaptive strategies to urbanization. The herb and shrub species had significantly higher starch and soluble sugar contents in urban than in suburban areas. Urbanization decreased the maximum photosynthetic rates and total chlorophyll contents of the canopies (Engelhardtia roxburghiana and Schima superba). The herbs (Lophatherum gracile and Alpinia chinensis) and shrubs (Ardisia quinquegona and Psychotria rubra) species in urban areas had significantly lower nitrogen (N) allocated in the cell wall and leaf δ15N values but higher heavy metal concentrations than those in suburban areas. The canopy and subcanopy (Diospyros morrisiana and Cratoxylum cochinchinense) species adapt to the urbanization via reducing resource acquisition but improving defense capacity, while the herb and shrub species improve resource acquisition to adapt to the urbanization. Our current studies indicated that functional groups affected the responses of plant adaptive strategies to the urbanization.
Are Grasses Really Useful for the Phytoremediation of Potentially Toxic Trace Elements? A Review
Authors: Rabêlo Flávio Henrique Silveira , Vangronsveld Jaco , Baker Alan J. M. , van der Ent Antony , Alleoni Luís Reynaldo Ferracciú
Published: December 2021

The pollution of soil, water, and air by potentially toxic trace elements poses risks to environmental and human health. For this reason, many chemical, physical, and biological processes of remediation have been developed to reduce the (available) trace element concentrations in the environment. Among those technologies, phytoremediation is an environmentally friendlyin situand cost-effective approach to remediate sites with low-to-moderate pollution with trace elements. However, not all species have the potential to be used for phytoremediation of trace element-polluted sites due to their morpho-physiological characteristics and low tolerance to toxicity induced by the trace elements. Grasses are prospective candidates due to their high biomass yields, fast growth, adaptations to infertile soils, and successive shoot regrowth after harvest. A large number of studies evaluating the processes related to the uptake, transport, accumulation, and toxicity of trace elements in grasses assessed for phytoremediation have been conducted. The aim of this review is (i) to synthesize the available information on the mechanisms involved in uptake, transport, accumulation, toxicity, and tolerance to trace elements in grasses; (ii) to identify suitable grasses for trace element phytoextraction, phytostabilization, and phytofiltration; (iii) to describe the main strategies used to improve trace element phytoremediation efficiency by grasses; and (iv) to point out the advantages, disadvantages, and perspectives for the use of grasses for phytoremediation of trace element-polluted soils.
Revealing the Genetic Components Responsible for the Unique Photosynthetic Stem Capability of the Wild Almond Prunus arabica (Olivier) Meikle
Authors: Brukental Hillel , Doron-Faigenboim Adi , Bar-Ya’akov Irit , Harel-Beja Rotem , Attia Ziv , Azoulay-Shemer Tamar , Holland Doron
Published: December 2021

Almond [Prunus dulcis (Mill.) D. A. Webb] is a major deciduous fruit tree crop worldwide. During dormancy, under warmer temperatures and inadequate chilling hours, the plant metabolic activity increases and may lead to carbohydrate deficiency. Prunus arabica (Olivier) Meikle is a bushy wild almond species known for its green, unbarked stem, which stays green even during the dormancy period. Our study revealed that P. arabica green stems assimilate significantly high rates of CO2 during the winter as compared to P. dulcis cv. Um el Fahem (U.E.F.) and may improve carbohydrate status throughout dormancy. To uncover the genetic inheritance and mechanism behind the P. arabica stem photosynthetic capability (SPC), a segregated F1 population was generated by crossing P. arabica to U.E.F. Both parent’s whole genome was sequenced, and SNP calling identified 4,887 informative SNPs for genotyping. A robust genetic map for U.E.F. and P. arabica was constructed (971 and 571 markers, respectively). QTL mapping and association study for the SPC phenotype revealed major QTL [log of odd (LOD) = 20.8] on chromosome 7 and another minor but significant QTL on chromosome 1 (LOD = 3.9). As expected, the P. arabica allele in the current loci significantly increased the SPC phenotype. Finally, a list of 64 candidate genes was generated. This work sets the stage for future research to investigate the mechanism regulating the SPC trait, how it affects the tree’s physiology, and its importance for breeding new cultivars better adapted to high winter temperatures.
Deciphering Main Climate and Edaphic Components Driving Oat Adaptation to Mediterranean Environments
Authors: Canales Francisco J. , Montilla-Bascón Gracia , Gallego-Sánchez Luis M. , Flores Fernando , Rispail Nicolas , Prats Elena
Published: December 2021

Oat, Avena sativa, is an important crop traditionally grown in cool-temperate regions. However, its cultivated area in the Mediterranean rim steadily increased during the last 20 years due to its good adaptation to a wide range of soils. Nevertheless, under Mediterranean cultivation conditions, oats have to face high temperatures and drought episodes that reduce its yield as compared with northern regions. Therefore, oat crop needs to be improved for adaptation to Mediterranean environments. In this work, we investigated the influence of climatic and edaphic variables on a collection of 709 Mediterranean landraces and cultivars growing under Mediterranean conditions. We performed genotype–environment interaction analysis using heritability-adjusted genotype plus genotype–environment biplot analyses to determine the best performing accessions. Further, their local adaptation to different environmental variables and the partial contribution of climate and edaphic factors to the different agronomic traits was determined through canonical correspondence, redundancy analysis, and variation partitioning. Here, we show that northern bred elite cultivars were not among the best performing accessions in Mediterranean environments, with several landraces outyielding these. While all the best performing cultivars had early flowering, this was not the case for all the best performing landraces, which showed different patterns of adaption to Mediterranean agroclimatic conditions. Thus, higher yielding landraces showed adaptation to moderate to low levels of rain during pre- and post-flowering periods and moderate to high temperature and radiation during post-flowering period. This analysis also highlights landraces adapted to more extreme environmental conditions. The study allowed the selection of oat genotypes adapted to different climate and edaphic factors, reducing undesired effect of environmental variables on agronomic traits and highlights the usefulness of variation partitioning for selecting genotypes adapted to specific climate and edaphic conditions.
TaMYB29: A Novel R2R3-MYB Transcription Factor Involved in Wheat Defense Against Stripe Rust
Authors: Zhu Xiaoxu , Li Xiang , He Qi , Guo Dongxiao , Liu Caiqi , Cao Junying , Wu Zhongyi , Kang Zhensheng , Wang Xiaojing
Published: December 2021

Members of the R2R3-MYB transcription factor superfamily have been implicated in plant development, improved disease resistance, and defense responses to several types of stresses. To study the function of TaMYB29 transcription factor—a member of the R2R3-MYB superfamily—in response to an avirulent race of stripe rust pathogen, Puccinia striiformis f. sp. tritici (Pst), we identified and cloned the TaMYB29 gene from wheat cultivar (cv.) AvS+Yr10 following infection with Pst. The TaMYB29 protein, comprising 261 amino acids, contains two highly conserved MYB domains. We first showed that TaMYB29 is a transcription factor, whose transcriptional levels are significantly induced by salicylic acid (SA), abscisic acid (ABA), jasmonic acid (JA), ethylene (ET), and Pst. The results showed that TaMYB29 is involved in the wheat response to stipe rust. The overexpression of the TaMYB29 gene resulted in the accumulation of reactive oxygen species (ROS) and pathogen-independent cell death in Nicotiana benthamiana leaves. The silencing of TaMYB29 gene in wheat cv. AvS+Yr10, containing the stripe rust resistance gene Yr10, promoted hyphae growth, significantly downregulated the expression of pathogenesis-related (PR) genes, and substantially reduced the wheat resistance to Pst compared with the non-silenced control. In addition, the accumulation of hydrogen peroxide (H2O2) significantly decreased, and the activity of catalase, an enzyme required for H2O2 scavenging, was elevated. Altogether, TaMYB29 positively regulates the defense response against stripe rust in wheat AvS+Yr10 by enhancing H2O2 accumulation, PR gene expression, and SA signaling pathway-induced cell death. These results provide new insights into the contribution of TaMYB29 to the defense response against rust pathogens in wheat.
A Cytochrome B5-Like Heme/Steroid Binding Domain Protein, PlCB5L1, Regulates Mycelial Growth, Pathogenicity and Oxidative Stress Tolerance in Peronophythora litchii
Authors: Li Wen , Li Peng , Zhou Xiaofan , Situ Junjian , Lin Yiming , Qiu Jiahui , Yuan Yuling , Xi Pinggen , Jiang Zide , Kong Guanghui
Published: December 2021

As an electron transport component, cytochrome b5 is an essential component of the Class II cytochrome P450 monooxygenation system and widely present in animals, plants, and fungi. However, the roles of Cyt-b5 domain proteins in pathogenic oomycetes remain unknown. Peronophythora litchii is an oomycete pathogen that causes litchi downy blight, the most destructive disease of litchi. In this study, we identified a gene, designated PlCB5L1, that encodes a Cyt-b5 domain protein in P. litchii, and characterized its function. PlCB5L1 is highly expressed in the zoospores, cysts, germinated cysts, and during early stages of infection. PlCB5L1 knockout mutants showed reduced growth rate and β-sitosterol utilization. Importantly, we also found that PlCB5L1 is required for the full pathogenicity of P. litchii. Compared with the wild-type strain, the PlCB5L1 mutants exhibited significantly higher tolerance to SDS and sorbitol, but impaired tolerance to cell wall stress, osmotic stress, and oxidative stress. Further, the expression of genes involved in oxidative stress tolerance, including peroxidase, cytochrome P450, and laccase genes, were down-regulated in PlCB5L1 mutants under oxidative stress. This is the first report that a Cyt-b5 domain protein contributes to the development, stress response, and pathogenicity in plant pathogenic oomycetes.
Advances and Perspectives of Transgenic Technology and Biotechnological Application in Forest Trees
Authors: Yin Yiyi , Wang Chun , Xiao Dandan , Liang Yanting , Wang Yanwei
Published: December 2021

Transgenic technology is increasingly used in forest-tree breeding to overcome the disadvantages of traditional breeding methods, such as a long breeding cycle, complex cultivation environment, and complicated procedures. By introducing exogenous DNA, genes tightly related or contributed to ideal traits—including insect, disease, and herbicide resistance—were transferred into diverse forest trees, and genetically modified (GM) trees including poplars were cultivated. It is beneficial to develop new varieties of GM trees of high quality and promote the genetic improvement of forests. However, the low transformation efficiency has hampered the cultivation of GM trees and the identification of the molecular genetic mechanism in forest trees compared to annual herbaceous plants such as Oryza sativa. In this study, we reviewed advances in transgenic technology of forest trees, including the principles, advantages and disadvantages of diverse genetic transformation methods, and their application for trait improvement. The review provides insight into the establishment and improvement of genetic transformation systems for forest tree species. Challenges and perspectives pertaining to the genetic transformation of forest trees are also discussed.
[Re]moving Bodies – A Shared Diminished Reality Installation for Exploring Relational Movement
Authors: Laroche Julien , Vuarnesson Loup , Endaltseva Alexandra , Dumit Joseph , Bachrach Asaf
Published: December 2021

In this article we explore an epistemic approach we name dis/embodiment and introduce “Articulations,” an interdisciplinary project bringing together Virtual Reality (VR) designers, cognitive scientists, dancers, anthropologists, and human–machine interaction specialists. According to Erin Manning, our sense of self and other emerges from processes of bodying and relational movement (becoming oneself by moving in relation with the world). The aim of the project is to exploit the potential of multi-person VR in order to explore the intersubjective dynamics of relational movement and bodying, and to do so with scientific, artistic and therapeutic purposes in mind. To achieve this bridge, we bring up a novel paradigm we name “Shared Diminished Reality”. It consists in using minimalist representation to instantiate users’ bodies in the virtual space. Instead of using humanoid avatars or full body skeletons, we reduce the representation of the moving bodies to three spheres whose trajectories reflect the tracking of the head and the two wrists. This “diminished”virtual rendition of the body-in-movement, we call dis/embodiment. It provides a simple but clear experience of one’s own responsive movement in relation to the world and other bodies. It also allows for subtle manipulations of bodies’ perceptual and cross-perceptual feedback and simplifies the tracking and the analysis of movements. After having introduced the epistemic framework, the basic architecture, and the empirical method informing the installation, we present and discuss, as a proof-of-concept, some data collected in a situated experiment at a science-art event. We investigate motion patterns observed in different experimental conditions (in which participants either could or could not see the representation of their own hands in the virtual space) and their relation with subjective reports collected. We conclude with reflection on further possibilities of our installation in exploring bodying and relational movement.
The Use of Questionable Research Practices to Survive in Academia Examined With Expert Elicitation, Prior-Data Conflicts, Bayes Factors for Replication Effects, and the Bayes Truth Serum
Authors: van de Schoot Rens , Winter Sonja D. , Griffioen Elian , Grimmelikhuijsen Stephan , Arts Ingrid , Veen Duco , Grandfield Elizabeth M. , Tummers Lars G.
Published: December 2021

The popularity and use of Bayesian methods have increased across many research domains. The current article demonstrates how some less familiar Bayesian methods can be used. Specifically, we applied expert elicitation, testing for prior-data conflicts, the Bayesian Truth Serum, and testing for replication effects via Bayes Factors in a series of four studies investigating the use of questionable research practices (QRPs). Scientifically fraudulent or unethical research practices have caused quite a stir in academia and beyond. Improving science starts with educating Ph.D. candidates: the scholars of tomorrow. In four studies concerning 765 Ph.D. candidates, we investigate whether Ph.D. candidates can differentiate between ethical and unethical or even fraudulent research practices. We probed the Ph.D.s’ willingness to publish research from such practices and tested whether this is influenced by (un)ethical behavior pressure from supervisors or peers. Furthermore, 36 academic leaders (deans, vice-deans, and heads of research) were interviewed and asked to predict what Ph.D.s would answer for different vignettes. Our study shows, and replicates, that some Ph.D. candidates are willing to publish results deriving from even blatant fraudulent behavior–data fabrication. Additionally, some academic leaders underestimated this behavior, which is alarming. Academic leaders have to keep in mind that Ph.D. candidates can be under more pressure than they realize and might be susceptible to using QRPs. As an inspiring example and to encourage others to make their Bayesian work reproducible, we published data, annotated scripts, and detailed output on the Open Science Framework (OSF).
Entering Into the Story: Implications for Emergent Literacy
Authors: Sperry Linda L. , Sperry Douglas E.
Published: December 2021

In this article we explore the ways in which three young children from a non-mainstream cultural group created stories with the assistance of their caregivers and siblings in the social contexts of their homes. We assert that these children’s oral narrations show us important dimensions of early experience with decontextualized content as practiced in their families that may offer suggestions for analysis of culturally sensitive experiences with literacy for all children. The dimensions we highlight are the tangibility of the elements around which the story is created, the interlocutor support children receive for beginning and continuing their stories, and the interaction between the storytelling process and the child’s self-interest. These three dimensions illustrate how children “enter” into stories and storytelling and broaden our understanding for fostering culturally sustaining pedagogy within schools.
What Language Disorders Reveal About the Mechanisms of Morphological Processing
Authors: Manouilidou Christina , Nerantzini Michaela , Chiappetta Brianne M. , Mesulam M. Marsel , Thompson Cynthia K.
Published: December 2021

We addressed an understudied topic in the literature of language disorders, that is, processing of derivational morphology, a domain which requires integration of semantic and syntactic knowledge. Current psycholinguistic literature suggests that word processing involves morpheme recognition, which occurs immediately upon encountering a complex word. Subsequent processes take place in order to interpret the combination of stem and affix. We investigated the abilities of individuals with agrammatic (PPA-G) and logopenic (PPA-L) variants of primary progressive aphasia (PPA) and individuals with stroke-induced agrammatic aphasia (StrAg) to process pseudowords which violate either the syntactic (word class) rules (*reheavy) or the semantic compatibility (argument structure specifications of the base form) rules (*reswim). To this end, we quantified aspects of word knowledge and explored how the distinct deficits of the populations under investigation affect their performance. Thirty brain-damaged individuals and 10 healthy controls participated in a lexical decision task. We hypothesized that the two agrammatic groups (PPA-G and StrAg) would have difficulties detecting syntactic violations, while no difficulties were expected for PPA-L. Accuracy and Reaction Time (RT) patterns indicated: the PPA-L group made fewer errors but yielded slower RTs compared to the two agrammatic groups which did not differ from one another. Accuracy rates suggest that individuals with PPA-L distinguish *reheavyfrom *reswim, reflecting access to and differential processing of syntactic vs. semantic violations. In contrast, the two agrammatic groups do not distinguish between *reheavyand *reswim. The lack of difference stems from a particularly impaired performance in detecting syntactic violations, as they were equally unsuccessful at detecting *reheavyand *reswim. Reduced grammatical abilities assessed through language measures are a significant predictor for this performance, suggesting that the “hardware” to process syntactic information is impaired. Therefore, they can only judge violations semantically where both *reheavyand *reswimfail to pass as semantically ill-formed. This finding further suggests that impaired grammatical knowledge can affect word level processing as well. Results are in line with the psycholinguistic literature which postulates the existence of various stages in accessing complex pseudowords, highlighting the contribution of syntactic/grammatical knowledge. Further, it points to the worth of studying impaired language performance for informing normal language processes.
An Association Between Montessori Education in Childhood and Adult Wellbeing
Authors: Lillard Angeline S. , Meyer M. Joseph , Vasc Dermina , Fukuda Eren
Published: December 2021

Wellbeing, or how people think and feel about their lives, predicts important life outcomes from happiness to health to longevity. Montessori pedagogy has features that enhance wellbeing contemporaneously and predictively, including self-determination, meaningful activities, and social stability. Here, 1905 adults, ages 18–81 (M= 36), filled out a large set of wellbeing scales followed by demographic information including type of school attended each year from 2 to 17. About half the sample had only attended conventional schools and the rest had attended Montessori for between 2 and 16 years (M= 8 years). To reduce the variable set, we first developed a measurement model of wellbeing using the survey data with exploratory then confirmatory factor analyses, arriving at four factors: general wellbeing, engagement, social trust, and self-confidence. A structural equation model that accounted for age, gender, race, childhood SES, and years in private school revealed that attending Montessori for at least two childhood years was associated with significantly higher adult wellbeing on all four factors. A second analysis found that the difference in wellbeing between Montessori and conventional schools existed even among the subsample that had exclusively attended private schools. A third analysis found that the more years one attended Montessori, the higher one’s wellbeing as an adult. Unmeasured selection effects could explain the results, in which case research should determine what third variable associated with Montessori schooling causes adult wellbeing. Several other limitations to the study are also discussed. Although some of these limitations need to be addressed, coupled with other research, including studies in which children were randomly assigned to Montessori schools, this study suggests that attending Montessori as a child might plausibly cause higher adult wellbeing.
How Children and Adolescents Perceive Their Coping With Home Learning in Times of COVID-19: A Mixed Method Approach
Authors: Simm Inga , Winklhofer Ursula , Naab Thorsten , Langmeyer Alexandra N. , Linberg Anja
Published: December 2021

With the COVID-19 pandemic, children and adolescents confronted a completely new learning situation. Instead of learning in class, they had to cope with home learning to achieve academically. This mixed-method study examines how children and adolescents in Germany perceive their coping success with home learning during the COVID-19 pandemic and how personal, school, family, and peer context factors relate to this self-perceived coping success. Quantitative data from an online survey of n=141 children (mage=10,8y) and n=266 adolescents (mage=15,2y; study 1) were used to analyze the questions with multiple regression analysis. With the qualitative data from 10 interviews with parents and their children (study 2), we examined the process of how school, family, and peer groups interact with students’ way of coping with home learning. Quantitative data show that most children and adolescents perceived their coping with home learning as successful and that school joy before COVID-19, parental support, and available equipment during home learning are still relevant for children, and family climate, calm place to learn, and equipment during home learning are important for adolescents learning at home. Qualitative data show that students apply individual ways of coping with home learning, where family and peers have a vital role, especially when contact with teachers is limited. Quantitative data confirm the importance of family context for students’ self-perceived coping success.