bluuebunny/crossref_metadata_2025 huggingface dataset:
Accurate Electro-Thermal Computational Model Design and Validation for Inverters of Automotive Electric Drivetrain Applications
Authors: Rasool Haaris , El Baghdadi Mohamed , Rauf Abdul Manan , Zhaksylyk Assel , D’hondt Thomas , Sarrazin Mathieu , Hegazy Omar
Published: May 2022

This paper proposes the fast and accurate electro-thermal model of the existing Simrod three-phase inverter for an electric vehicle (EV) application. The research focuses on analytical and dynamic electro-thermal models of inverters that can be applied for multi-applications. The optimal design approach of passive filters is presented for the DC and AC sides of the inverter. The analytical model has been established, including a mathematical representation of the inverter and induction motor (IM). The high-fidelity electro-thermal simulation model of an inverter with integrated power loss and thermal model is established. The state-space thermal model (for the IRFS4115PbF device) has been created and incorporated into the MATLAB simulation. The simulation model is then validated with the PLECS software-based thermal model to confirm the accuracy. Indirect field-oriented control (IFOC) is designed for squirrel-cage IM at a maximum power rating of 45 kW and implemented on MATLAB/Simulink. The comparative analysis between the real and simulated results is performed to validate the simulation model at a specific speed, torque, and current. Furthermore, the electro-thermal simulation model has been validated with experimental data using efficiency and temperature comparison. The developed simulation model is beneficial for designing, optimizing, and developing advanced technology-based inverters to achieve higher efficiency at a particular operating range of temperature and power quality. The new European driving cycle (NEDC) speed profile simulation results are demonstrated.
Mathematical Modeling Research Output Impacting New Technological Development: An Axiomatization to Build Novelty
Authors: Bajuri Mohd Razip , Siri Zailan , Abdullah Mohd Nor Syahrir
Published: May 2022

The mathematical modeling of research-based output impacting new technology development is crucial for a developing country. However, the complexity of modern mathematical modeling research output makes it unclear over how it can impact the development of new technology. Therefore, this study aims to explore, categorize and formulize the axioms of mathematical modeling research output that impacts the development of new technology. Seven participants were involved in this research. Interviews were conducted to explore their remarkable mathematical modeling output and how the output can impact the development of new technology. The categorization axioms are: i. mathematical modeling for theorizing, ii. mathematical modeling for simulations, iii. mathematical modeling for useable innovation and iv. patent and product commercialization. Finally, the categorization can be formulized as an axiom of mathematical modeling novelty, which is the desired research output. Moreover, patents and commercialization are the elements that mathematical modeling should possess for new technological development. The limited number of participants involved in this study makes this study formulation limited to only some types of mathematical modeling output. However, this substantive formulation could give some ideas in proposing the path and processes on how to enhance the effort for society to develop the culture of mathematical modeling in developing new technology.
Decellularized Human Umbilical Tissue-Derived Hydrogels Promote Proliferation and Chondrogenic Differentiation of Mesenchymal Stem Cells
Authors: Ramzan Faiza , Ekram Sobia , Frazier Trivia , Salim Asmat , Mohiuddin Omair Anwar , Khan Irfan
Published: May 2022

Tissue engineering is a promising approach for the repair and regeneration of cartilaginous tissue. Appropriate three-dimensional scaffolding materials that mimic cartilage are ideal for the repair of chondral defects. The emerging decellularized tissue-based scaffolds have the potential to provide essential biochemical signals and structural integrity, which mimics the natural tissue environment and directs cellular fate. Umbilical cord-derived hydrogels function as 3D scaffolding material, which support adherence, proliferation, migration, and differentiation of cells due to their similar biochemical composition to cartilage. Therefore, the present study aimed to establish a protocol for the formulation of a hydrogel from decellularized human umbilical cord (DUC) tissue, and assess its application in the proliferation and differentiation of UC-MSCs along chondrogenic lineage. The results showed that the umbilical cord was efficiently decellularized. Subsequently, DUC hydrogel was prepared, and in vitro chondral differentiation of MSCs seeded on the scaffold was determined. The developed protocol efficiently removed the cellular and nuclear content while retaining the extracellular matrix (ECM). DUC tissue, pre-gel, and hydrogels were evaluated by FTIR spectroscopy, which confirmed the gelation from pre-gel to hydrogel. SEM analysis revealed the fibril morphology and porosity of the DUC hydrogel. Calcein AM and Alamar blue assays confirmed the MSC survival, attachment, and proliferation in the DUC hydrogels. Following seeding of UC-MSCs in the hydrogels, they were cultured in stromal or chondrogenic media for 28 days, and the expression of chondrogenic marker genes including TGF-β1, BMP2, SOX-9, SIX-1, GDF-5, and AGGRECAN was significantly increased (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Moreover, the hydrogel concentration was found to significantly affect the expression of chondrogenic marker genes. The overall results indicate that the DUC-hydrogel is compatible with MSCs and supports their chondrogenic differentiation in vitro.
Simplified Convolutional Neural Network Application for Cervix Type Classification via Colposcopic Images
Authors: Pavlov Vitalii , Fyodorov Stanislav , Zavjalov Sergey , Pervunina Tatiana , Govorov Igor , Komlichenko Eduard , Deynega Viktor , Artemenko Veronika
Published: May 2022

The inner parts of the human body are usually inspected endoscopically using special equipment. For instance, each part of the female reproductive system can be examined endoscopically (laparoscopy, hysteroscopy, and colposcopy). The primary purpose of colposcopy is the early detection of malignant lesions of the cervix. Cervical cancer (CC) is one of the most common cancers in women worldwide, especially in middle- and low-income countries. Therefore, there is a growing demand for approaches that aim to detect precancerous lesions, ideally without quality loss. Despite its high efficiency, this method has some disadvantages, including subjectivity and pronounced dependence on the operator’s experience. The objective of the current work is to propose an alternative to overcoming these limitations by utilizing the neural network approach. The classifier is trained to recognize and classify lesions. The classifier has a high recognition accuracy and a low computational complexity. The classification accuracies for the classes normal, LSIL, HSIL, and suspicious for invasion were 95.46%, 79.78%, 94.16%, and 97.09%, respectively. We argue that the proposed architecture is simpler than those discussed in other articles due to the use of the global averaging level of the pool. Therefore, the classifier can be implemented on low-power computing platforms at a reasonable cost.
The Influence of a Shoe’s Heel-Toe Drop on Gait Parameters during the Third Trimester of Pregnancy
Authors: Li Xin , Lu Zhenghui , Sun Dong , Xuan Rongrong , Zheng Zhiyi , Gu Yaodong
Published: May 2022

Background: Changes in physical shape and body mass during pregnancy may increase the risk of walking falls. Shoes can protect and enhance the inherent function of the foot, helping to maintain dynamic and static stability. Methods: Sixteen women during the third trimester of pregnancy participated in this study to investigate the effect of negative heel shoes (NHS), positive heel shoes (PHS), and normal shoes (NS) on spatiotemporal parameters, ground reaction force (GRF), and stability. Differences in spatiotemporal parameter, GRF, and center of pressure (COP) between footwear conditions were examined using Statistical Parametric Mapping (SPM) and repeated measures analyses of variance (ANOVA). Results: The walking speed and step length increased with the increase in heel-toe drop. The anterior-posterior (AP)-COP in NHS decreased significantly (p < 0.001). When wearing NHS, peak posterior angles were significantly lower than NS and PHS (p < 0.05). Conclusions: The results show that changing the heel-toe drop can significantly affect the gait pattern of pregnant women. Understanding the gait patterns of pregnant women wearing shoes with different heel-toe drops is very important for reducing the risk of injury and equipment design.
3D Bioprinting of Prevascularized Full-Thickness Gelatin-Alginate Structures with Embedded Co-Cultures
Authors: Böttcher Bastian , Pflieger Astrid , Schumacher Jan , Jungnickel Berit , Feller Karl-Heinz
Published: May 2022

The use of bioprinting allows the creation of complex three-dimensional cell laden grafts with spatial placements of different cell lines. However, a major challenge is insufficient nutrient transfer, especially with the increased size of the graft causing necrosis and reduced proliferation. A possibility to improve nutrient support is the integration of tubular structures for reducing diffusion paths. In this study the influence of prevascularization in full-thickness grafts on cell growth with a variation of cultivation style and cellular composition was investigated. To perform this, the rheological properties of the used gelatin-alginate hydrogel as well as possibilities to improve growth conditions in the hydrogel were assessed. Prevascularized grafts were manufactured using a pneumatic extrusion-based bioprinter with a coaxial extrusion tool. The prevascularized grafts were statically and dynamically cultured with a monoculture of HepG2 cells. Additionally, a co-culture of HepG2 cells, fibroblasts and HUVEC-TERT2 was created while HUVEC-TERT2s were concentrically placed around the hollow channels. A static culture of prevascularized grafts showed short-term improvements in cell proliferation compared to avascular grafts, while a perfusion-based culture showed improvements in mid-term cultivation times. The cultivation of the co-culture indicated the formation of vascular structures from the hollow channels toward avascular areas. According to these results, the integration of prevascular structures show beneficial effects for the in vitro cultivation of bioprinted grafts for which its impact can be increased in larger grafts.
Aerosol Nutrients and Their Biological Influence on the Northwest Pacific Ocean (NWPO) and Its Marginal Seas
Authors: Guo Cui , Zhou Yao , Zhou Hongyan , Su Chang , Kong Liangliang
Published: May 2022

Atmospheric deposition is recognized as a significant source of nutrients in the surface ocean. The East Asia region is among the largest sources of aerosol emissions in the world, due to its large industrial, agricultural, and energy production. Thus, East Asian aerosols contain a large proportion of anthropogenic particles that are characterized by small size, complex composition, and high nutrient dissolution, resulting in important influences on marine microbes and biogeochemical cycles in the downwind areas of the northwest Pacific Ocean (NWPO). By using remote sensing, modeling, and incubation experimental methods, enhanced primary production due to the East Asian aerosol input has been observed in the NWPO, with subsequent promotion and inhibition impacts on different phytoplankton taxa. Changes of bacterial activity and diversity also occur in response to aerosol input. The impact of East Asian aerosol loadings is closely related to the amount and composition of the aerosol deposition as well as the hydrological condition of the receiving seawater. Here, we review the current state of knowledge on the atmospheric nutrients and the effects of the East Asian aerosols on microbes in the NWPO region. Future research perspectives are also proposed.
Cortisol Regulates Cerebral Mitochondrial Oxidative Phosphorylation and Morphology of the Brain in a Region-Specific Manner in the Ovine Fetus
Authors: Davies Katie L. , Smith Danielle J. , El-Bacha Tatiana , Wooding Peter F. P. , Forhead Alison J. , Murray Andrew J. , Fowden Abigail L. , Camm Emily J.
Published: May 2022

In adults, glucocorticoids are stress hormones that act, partly, through actions on mitochondrial oxidative phosphorylation (OXPHOS) to increase energy availability. Before birth, glucocorticoids are primarily maturational signals that prepare the fetus for new postnatal challenges. However, the role of the normal prepartum glucocorticoid rise in preparing mitochondria for the increased postnatal energy demands remains largely unknown. This study examined the effect of physiological increases in the fetal cortisol concentration on cerebral mitochondrial OXPHOS capacity near term (~130 days gestation, term ~145 days gestation). Fetal sheep were infused with saline or cortisol for 5 days at ~0.8 of gestation before the mitochondrial content, respiratory rates, abundance of the electron transfer system proteins and OXPHOS efficiency were measured in their cortex and cerebellum. Cerebral morphology was assessed by immunohistochemistry and stereology. Cortisol treatment increased the mitochondrial content, while decreasing Complex I-linked respiration in the cerebellum. There was no effect on the cortical mitochondrial OXPHOS capacity. Cortisol infusion had regional effects on cerebral morphology, with increased myelination in the cerebrum. The findings demonstrate the importance of cortisol in regulating the cerebral mitochondrial OXPHOS capacity prenatally and have implications for infants born preterm or after glucocorticoid overexposure due to pregnancy complications or clinical treatment.
Mechanisms of Regenerative Potential Activation in Cardiac Mesenchymal Cells
Authors: Docshin Pavel M. , Karpov Andrei A. , Mametov Malik V. , Ivkin Dmitry Y. , Kostareva Anna A. , Malashicheva Anna B.
Published: May 2022

Recovery of the contractile function of the heart and the regeneration of the myocardium after ischemic injury are contemporary issues in regenerative medicine and cell biology. This study aimed to analyze early transcriptional events in cardiac tissue after infarction and to explore the cell population that can be isolated from myocardial tissue. We induced myocardial infarction in Wistar rats by permanent ligation of the left coronary artery and showed a change in the expression pattern of Notch-associated genes and Bmp2/Runx2 in post-MI tissues using RNA sequencing and RT-PCR. We obtained primary cardiac mesenchymal cell (CMC) cultures from postinfarction myocardium by enzymatic dissociation of tissues, which retained part of the activation stimulus and had a pronounced proliferative potential, assessed using a “xCELLigence” real-time system. Hypoxia in vitro also causes healthy CMCs to overexpress Notch-associated genes and Bmp2/Runx2. Exogenous activation of the Notch signaling pathway by lentiviral transduction of healthy CMCs resulted in a dose-dependent activation of the Runx2 transcription factor but did not affect the activity of the Bmp2 factor. Thus, the results of this study showed that acute hypoxic stress could cause short-term activation of the embryonic signaling pathways Notch and Bmp in CMCs, and this interaction is closely related to the processes of early myocardial remodeling after a heart attack. The ability to correctly modulate and control the corresponding signals in the heart can help increase the regenerative capacity of the myocardium before the formation of fibrotic conditions.
A Review of Physical and Digital Mock-Up Applications in Healthcare Building Development
Authors: Lu Jun , Fu Changfeng , Zhou Tongyu , Xie Jing , Loo Yat Ming
Published: May 2022

Mock-up simulation is a design or human factor research method to help designers identify key design issues and factors of a product or environment. This paper discusses physical mock-up (PMU) and digital mock-up (DMU) applications in healthcare building development through a narrative literature review. The following questions are addressed in this paper: what would the purposes of using PMU or DMU simulations be? At which phase of a hospital design would a PMU or DMU simulation be used? What methods can be used to conduct PMU and DMU simulations? The paper discusses the advantages and disadvantages of these two mock-up methods and highlights the importance of clinical staff’s involvement in mock-up simulations. It gives recommendations for the design practitioners or project managers of healthcare building development recommendations to implement these two mock-up methods in healthcare building development projects.
Study of the Durability Damage of Ultrahigh Toughness Fiber Concrete Based on Grayscale Prediction and the Weibull Model
Authors: Wang Chen , Fu Pei , Liu Zeli , Xu Ziling , Wen Tao , Zhu Yingying , Long Yuhua , Jiang Jiuhong
Published: May 2022

The purpose of this research is to investigate the durability damage law for ultrahigh toughness cementitious composites (UHTCCs) under freeze–thaw environments and impact resistance. In this study, UHTCCs with fiber length-to-diameter ratios of 5/30, 8/30, 12/20, 12/30 and 12/48 were tested for impact resistance and freeze–thaw cycles. The freeze–thaw cycle process and impact resistance process for UHTCC are comprehensively analyzed and evaluated in terms of mass loss, compressive strength loss, relative dynamic elastic modulus loss and impact resistance number. The freeze–thaw damage prediction model for the relative dynamic elastic modulus of the UHTCC is established based on the regularity of the measured data for the relative dynamic elastic modulus of UHTCC and also on the GM(1,1) power model. The accuracy and reliability of the GM(1,1) power model is analyzed using the relative error, absolute correlation degree, mean variance and probability of small errors. According to the evolution law of the impact resistance number of the UHTCC, the impact damage prediction model for UHTCC is established based on the Weibull distribution model, and the accuracy of the model is analyzed by using the decision coefficient R2. The results show that UHTCC has high durability performance, and the durability performance of UHTCC at a length-diameter ratio of 12/48 is optimal. The freeze–thaw damage evolution model and impact damage evolution model established in this research are sufficiently realistic, the average relative error of the GM(1,1) power model is less than 5%, and the coefficient of determination R2 of the Weibull distribution model is greater than 0.93, which effectively reflects the damage development process for concrete under freeze–thaw and impact environment with high fitting accuracy.
Infrastructural Requirements and Regulatory Challenges of a Sustainable Urban Air Mobility Ecosystem
Authors: Takacs Arpad , Haidegger Tamas
Published: May 2022

The United Nations has long put on the discussion agenda the sustainability challenges of urbanization, which have both direct and indirect effects on future regulation strategies. Undoubtedly, most initiatives target better quality of life, improved access to services & goods and environment protection. As commercial aerial urban transportation may become a feasible research goal in the near future, the connection possibilities between cities and regions scale up. It is expected that the growing number of vertical takeoff & landing vehicles used for passenger and goods transportation will change the infrastructure of the cities, and will have a significant effect on the cityscapes as well. In addition to the widely discussed regulatory and safety issues, the introduction of elevated traffic also raises environmental concerns, which influences the existing and required service and control infrastructure, and thus significantly affects sustainability. This paper provides narrated overview of the most common aspects of safety, licensing and regulations for passenger vertical takeoff & landing vehicles, and highlights the most important aspects of infrastructure planning, design and operation, which should be taken into account to maintain and efficiently operate this new way of transportation, leading to a sustainable urban air mobility ecosystem.
The HIF-1α as a Potent Inducer of the Hallmarks in Gastric Cancer
Authors: Ucaryilmaz Metin Cemre , Ozcan Gulnihal
Published: May 2022

Hypoxia is the principal architect of the topographic heterogeneity in tumors. Hypoxia-inducible factor-1α (HIF-1α) reinforces all hallmarks of cancer and donates cancer cells with more aggressive characteristics at hypoxic niches. HIF-1α potently induces sustained growth factor signaling, angiogenesis, epithelial–mesenchymal transition, and replicative immortality. Hypoxia leads to the selection of cancer cells that evade growth suppressors or apoptotic triggers and deregulates cellular energetics. HIF-1α is also associated with genetic instability, tumor-promoting inflammation, and escape from immunity. Therefore, HIF-1α may be an important therapeutic target in cancer. Despite that, the drug market lacks safe and efficacious anti-HIF-1α molecules, raising the quest for fully unveiling the complex interactome of HIF-1α in cancer to discover more effective strategies. The knowledge gap is even wider in gastric cancer, where the number of studies on hypoxia is relatively low compared to other well-dissected cancers. A comprehensive review of the molecular mechanisms by which HIF-1α induces gastric cancer hallmarks could provide a broad perspective to the investigators and reveal missing links to explore in future studies. Thus, here we review the impact of HIF-1α on the cancer hallmarks with a specific focus on gastric cancer.
LncRNA JPX Promotes Esophageal Squamous Cell Carcinoma Progression by Targeting miR-516b-5p/VEGFA Axis
Authors: He Yi , Hua Rong , Yang Yang , Li Bin , Guo Xufeng , Li Zhigang
Published: May 2022

Long non-coding RNAs (lncRNAs) are reported act as important regulators in various types of cancer. LncRNA JPX was identified as an oncogenic regulator in lung cancer. However, the function of JPX in the progression of esophageal squamous cell carcinoma (ESCC) remains unclear. In the present study, we found JPX was highly expressed in esophageal tissue from ESCC patients. Functional assays demonstrated that JPX promoted ESCC cell proliferation, migration, and invasion in vitro, and accelerated tumor growth in vivo. Mechanistically, the results showed that JPX functioned as a sponge of miR-516b-5p, which targeted vascular endothelial growth factor A (VEGFA) in ESCC cells. Interactions between miR-516b-5p and JPX or VEGFA were confirmed by luciferase reporter assays. Inhibition of JPX significantly attenuated the cell growth and mobility ability of ESCC cells in vitro. In addition, overexpression of miR-516b-5p abrogated JPX-enhanced proliferation, migration, invasion, and angiogenesis of ESCC cells. Our study demonstrated that JPX played an important role in promoting ESCC progression via the miR-516b-5p/VEGFA pathway, which might serve as a promising novel diagnostic biomarker and therapeutic target for ESCC in clinic.
SAR131675, a VEGRF3 Inhibitor, Modulates the Immune Response and Reduces the Growth of Colorectal Cancer Liver Metastasis
Authors: Walsh Katrina A. , Kastrappis Georgios , Fifis Theodora , Paolini Rita , Christophi Christopher , Perini Marcos V.
Published: May 2022

Most patients with colorectal cancer (CRC) develop metastases, predominantly in the liver (CLM). Targeted therapies are being investigated to improve current CLM treatments. This study tested the effectiveness of SAR131675, a selective VEGFR-3 tyrosine kinase inhibitor, to inhibit CLM in a murine model. Following intrasplenic induction of CLM, mice were treated daily with SAR131675. Tumor growth and immune infiltrates into tumor and liver tissues were assessed at 10-, 16- and 22-days post tumor induction by stereology, IHC and flow cytometry. SAR151675 treatment significantly reduced tumor burden and F4/80+ macrophages in the liver tissues. Analysis of immune cell infiltrates in liver showed tissue that at day 22, had the proportion of CD45+ leukocytes significantly reduced, particularly myeloid cells. Analysis of myeloid cells (CD11b+ CD45+) indicated that the proportion of F4/80− Ly6Clow was significantly reduced, including a predominate PD-L1+ subset, while CD3+ T cells increased, particularly CD8+ PD1+, reflected by an increase in the CD8+:CD4+ T cell ratio. In the tumor tissue SAR11675 treatment reduced the predominant population of F4/80+ Ly6Clo and increased CD4+ T cells. These results suggest that SAR131675 alters the immune composition within tumor and the surrounding liver in the later stages of development, resulting in a less immunosuppressive environment. This immunomodulation effect may contribute to the suppression of tumor growth.
PET-CT in Clinical Adult Oncology—V. Head and Neck and Neuro Oncology
Authors: Wiggins Richard H. , Hoffman John M. , Fine Gabriel C. , Covington Matthew F. , Salem Ahmed Ebada , Koppula Bhasker R. , Morton Kathryn A.
Published: May 2022

PET-CT is an advanced imaging modality with many oncologic applications, including staging, assessment of response to therapy, restaging, and longitudinal surveillance for recurrence. The goal of this series of six review articles is to provide practical information to providers and imaging professionals regarding the best use of PET-CT for specific oncologic indications, and the potential pitfalls and nuances that characterize these applications. In addition, key tumor-specific clinical information and representative PET-CT images are provided to outline the role that PET-CT plays in the management of oncology patients. Hundreds of different types of tumors exist, both pediatric and adult. A discussion of the role of FDG PET for all of these is beyond the scope of this review. Rather, this series of articles focuses on the most common adult malignancies that may be encountered in clinical practice. It also focuses on FDA-approved and clinically available radiopharmaceuticals, rather than research tracers or those requiring a local cyclotron. The fifth review article in this series focuses on PET-CT imaging in head and neck tumors, as well as brain tumors. Common normal variants, key anatomic features, and benign mimics of these tumors are reviewed. The goal of this review article is to provide the imaging professional with guidance in the interpretation of PET-CT for the more common head and neck malignancies and neuro oncology, and to inform the referring providers so that they can have realistic expectations of the value and limitations of PET-CT for the specific type of tumor being addressed.
Catalysts Derived from Nickel-Containing Layered Double Hydroxides for Aqueous-Phase Furfural Hydrogenation
Authors: Belskaya Olga B. , Mironenko Roman M. , Gulyaeva Tatiana I. , Trenikhin Mikhail V. , Muromtsev Ivan V. , Trubina Svetlana V. , Zvereva Valentina V. , Likholobov Vladimir A.
Published: May 2022

Changes in the structural and textural properties of NiAl-layered double hydroxides (LDHs) (with 2–4 molar ratios of metals) and state of nickel that occur in different steps of the synthesis of nickel catalysts were studied using XRD, thermal analysis, TPR, low-temperature nitrogen adsorption, XANES, EXAFS, and electron microscopy methods. Relations between nickel content, catalyst reduction conditions, state of nickel, and its catalytic properties were revealed. It was shown that the use of NiAl LDH as the catalyst precursor even at a high content of active metal allows for the obtaining of the dispersed particles of supported nickel that are active in the aqueous-phase hydrogenation of furfural. The catalyst activity and conversion of furfural were found to increase with elevation of the catalyst reduction temperature and the corresponding growth of the fraction of reduced nickel. However, a lower reduction temperature (500 °C) makes it possible to form smaller nickel particles with the size of 4–6 nm, and a high Ni content (Ni:Al = 4) can be used to obtain the active Ni@NiAlOx catalyst. Under mild reaction conditions (90 °C, 2.0 MPa), the furfural conversion reached 93%, and furfuryl alcohol was formed with the selectivity of 70%. Under more severe reaction conditions (150 °C, 3.0 MPa), complete conversion of furfural was achieved, and cyclopentanol and tetrahydrofurfuryl alcohol were the main hydrogenation products.
Effect of Potassium Promoter on the Performance of Nickel-Based Catalysts Supported on MnOx in Steam Reforming of Ethanol
Authors: Greluk Magdalena , Rotko Marek , Słowik Grzegorz , Turczyniak-Surdacka Sylwia , Grzybek Gabriela , Góra-Marek Kinga , Kotarba Andrzej
Published: May 2022

The effect of a potassium promoter on the stability of and resistance to a carbon deposit formation on the Ni/MnOx catalyst under SRE conditions was studied at 420 °C for different H2O/EtOH molar ratios in the range from 4/1 to 12/1. The catalysts were prepared by the impregnation method and characterized using several techniques to study their textural, structural, and redox properties before being tested in a SRE reaction. The catalytic tests indicated that the addition of a low amount of potassium (1.6 wt.%) allows a catalyst with high stability to be obtained, which was ascribed to high resistance to carbon formation. The restriction of the amount of carbon deposits originates from the potassium presence on the Ni surface, which leads to (i) a decrease in the number of active sites available for methane decomposition and (ii) an increase in the rate of the steam gasification of carbon formed during SRE reactions.
Poloxamer 407 Induces Hypertriglyceridemia but Decreases Atherosclerosis in Ldlr−/− Mice
Authors: Peng Xueying , Lian Zeqin , Perrard Xiao-Yuan Dai , Xiao Yunjie , Ni Jing , O’Brien Veronica , Dong Henry , Pownall Henry J. , Ballantyne Christie M. , Wu Huaizhu
Published: May 2022

Background: Hypertriglyceridemia (HTG) increases the risk for atherosclerotic cardiovascular disease, but underlying mechanisms are incompletely understood. Circulating monocytes play an important role in atherogenesis by infiltrating arterial walls, where they differentiate into macrophages. We tested the hypothesis that HTG is mechanistically linked to atherogenesis by altering the monocyte phenotype and infiltration into atherosclerotic lesions in a model of diet-induced atherogenesis in Ldlr−/− mice. Methods: HTG was induced in male Ldlr−/− mice, fed a Western, high-fat high-cholesterol diet, by daily injection of poloxamer 407 (P407), a lipoprotein lipase inhibitor, for seven weeks. Atherosclerosis, monocyte phenotypes, and monocyte migration into atherosclerotic lesions were determined by well-validated methods. Results: Compared with the saline control, P407 injection in Ldlr−/− mice rapidly induced profound and persistent HTG, modestly elevated plasma cholesterol levels, and increased levels of triglyceride and cholesterol carried in very-low-density lipoprotein and low-density lipoprotein. Unexpectedly, mice receiving P407 versus saline control showed less atherosclerosis. Following induction of HTG by P407, CD36+ (also CD11c+), but not CD36− (CD11c−), monocytes showed early increases in lipid accumulation, but the number of CD36+ (not CD36−) monocytes was dramatically decreased afterwards in the circulation until the end of the test. Concurrently, CD36+ (CD11c+) monocyte migration into atherosclerotic lesions was also reduced in mice receiving P407 versus controls. Conclusions: P407 induced severe HTG, but reduced atherosclerosis, in Ldlr−/− mice, possibly because of profound reductions of circulating CD36+ (CD11c+) monocytes, leading to decreased monocyte migration into atherosclerotic lesions.
Stem Cell-Based Regenerative Therapy and Derived Products in COPD: A Systematic Review and Meta-Analysis
Authors: Calzetta Luigino , Aiello Marina , Frizzelli Annalisa , Camardelli Francesca , Cazzola Mario , Rogliani Paola , Chetta Alfredo
Published: May 2022

COPD is an incurable disorder, characterized by a progressive alveolar tissue destruction and defective mechanisms of repair and defense leading to emphysema. Currently, treatment for COPD is exclusively symptomatic; therefore, stem cell-based therapies represent a promising therapeutic approach to regenerate damaged structures of the respiratory system and restore lung function. The aim of this study was to provide a quantitative synthesis of the efficacy profile of stem cell-based regenerative therapies and derived products in COPD patients. A systematic review and meta-analysis was performed according to PRISMA-P. Data from 371 COPD patients were extracted from 11 studies. Active treatments elicited a strong tendency towards significance in FEV1 improvement (+71 mL 95% CI -2–145; p = 0.056) and significantly increased 6MWT (52 m 95% CI 18–87; p < 0.05) vs. baseline or control. Active treatments did not reduce the risk of hospitalization due to acute exacerbations (RR 0.77 95% CI 0.40–1.49; p > 0.05). This study suggests that stem cell-based regenerative therapies and derived products may be effective to treat COPD patients, but the current evidence comes from small clinical trials. Large and well-designed randomized controlled trials are needed to really quantify the beneficial impact of stem cell-based regenerative therapy and derived products in COPD.
Immune Microenvironment of Muscular-Invasive Urothelial Carcinoma: The Link to Tumor Immune Cycle and Prognosis
Authors: Stakhovskyi Oleksandr , Kobyliak Nazarii , Voylenko Oleg , Stakhovskyi Eduard , Ponomarchuk Roman , Sulaieva Oksana
Published: May 2022

In this study, we investigated the relationship between the tumor immune microenvironment (TIME), histological differentiation and hypoxia in patients with muscular-invasive urothelial carcinomas (MIUC) after radical cystectomy. Forty-two cases of pT2-3N0M0 MIUCs underwent clinical, histological and immunohistochemical evaluation by counting CD8+, FOXP3+, CD68+, CD163+ cells and polymorphonuclear leukocytes (PMN) in intra-tumoral and peritumoral areas, assessing PD-L1 and GLUT1 expression for defining the impact of tumor immune contexture on patients’ outcomes. Five-year survival rates and overall survival were calculated. Most of the MIUCs demonstrated the immune-desert or immune-excluded TIME, reflecting altered mechanisms of T-cells’ activation or traffic into tumors. Tumor immune contexture was closely related to histological differentiation. CD8+ cells were scant in MIUCs with papillary and squamous differentiation, while basal-like or mesenchymal-like histological differentiation was associated with increased density of CD8+ cells. A high rate of PD-L1 expression (47.6%) was not related to immune cell infiltration. M2-macrophages predominated under CD8+ lymphocytes. The abundance of PMN and CD163+ macrophages in MIUCs was associated with high GLUT1 expression. CD8+, CD68+, FOXP3+ cells and PD-L1 status did not affect patients’ outcomes, while high CD163+ density and PMN infiltration were associated with the unfavorable outcome of patients with MIUC. These data drive the hypothesis that in MIUC, immune escape mechanisms are shifted towards the role of the innate immunity cells rather than CD8+ lymphocytes’ functioning.
Renal Ischemia Induces Epigenetic Changes in Apoptotic, Proteolytic, and Mitochondrial Genes in Swine Scattered Tubular-like Cells
Authors: Rajagopalan Kamalnath S. , Glasstetter Logan M. , Zhu Xiang-Yang , Thaler Roman , Tang Hui , Jordan Kyra L. , Saadiq Ishran M. , Herrmann Sandra M. , Chade Alejandro R. , Irazabal Maria V. , Lerman Lilach O. , Eirin Alfonso
Published: May 2022

Background: Scattered tubular-like cells (STCs) are dedifferentiated renal tubular cells endowed with progenitor-like characteristics to repair injured parenchymal cells. STCs may be damaged and rendered ineffective by renal artery stenosis (RAS), but the underlying processes remain unclear. We hypothesized that RAS alters the epigenetic landscape on DNA and the ensuing gene transcriptional profile of swine STCs. Methods: CD24+/CD133+ STCs were isolated from pig kidneys after 10 weeks of RAS or sham (n = 3 each) and their whole 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) profiles were examined by 5mC and 5hmC immunoprecipitation sequencing (MeDIP-/hMeDIP-seq, respectively). A subsequent integrated (MeDIP/hMeDIP-seq/mRNA-seq) analysis was performed by comparing all online available gene sets using Gene Set Enrichment Analysis. Apoptosis, proteolysis, and mitochondrial structure and function were subsequently evaluated in vitro. Results: Differential expression (DE) analysis revealed 239 genes with higher and 236 with lower 5mC levels and 275 genes with higher and 315 with lower 5hmC levels in RAS-STCs compared to Normal-STCs (fold change ≥1.4 or ≤0.7, p ≤ 0.05). Integrated MeDIP-/hMeDIP-seq/mRNA-seq analysis identified several overlapping (DE-5mC/mRNA and DE-5hmC/mRNA levels) genes primarily implicated in apoptosis, proteolysis, and mitochondrial functions. Furthermore, RAS-STCs exhibited decreased apoptosis, mitochondrial matrix density, and ATP production, and increased intracellular amino acid concentration and ubiquitin expression. Conclusions: Renal ischemia induces epigenetic changes in apoptosis-, proteolysis-, and mitochondria-related genes, which correlate with alterations in the transcriptomic profile and corresponding function of swine STCs. These observations may contribute to developing novel targeted interventions to preserve the reparative potency of STCs in renal disease.
Kinetics of Thermal Decomposition of Particulate Samples of MgCO3: Experiments and Models
Authors: Mancarella Francesca , D’Elia Marcella , Micca Longo Gaia , Longo Savino , Orofino Vincenzo
Published: May 2022

In this work, we study the kinetics of thermal decomposition of MgCO3 in the form of particles of known size. In the experiments, the material is heated to a known temperature in a vacuum oven, and it is characterized, both before and after heating, by infrared spectroscopy and gravimetry. The agreement between the results of the two techniques is excellent. These results are rationalized by means of a model based on Languir’s law, and the comparison with the experiments allows us to estimate the activation energy of the process. The reabsorption of atmospheric water by the oxide is shown spectroscopically, finding that is strongly influenced by the temperature of the process.
Barriers to the Successful Health Care Transition of Patients with Kidney Disease: A Mixed-Methods Study on the Perspectives of Adult Nephrologists
Authors: Prüfe Jenny , Pape Lars , Kreuzer Martin
Published: May 2022

The transition from paediatric to adult-based health care is a challenging period bearing a high risk of medication nonadherence and transplant loss in adolescents and young adults after kidney transplantation. Successful transition asks for the cooperation of many, not least the adult physicians. Yet little is known about their thoughts and attitudes on the transition. We conducted a cross-sectional mixed-methods study, inviting all nephrologists registered with the German Society of Nephrology. A total of 119/1984 nephrologists answered an online survey, and 9 nephrologists participated in expert interviews on transition experiences and perceived barriers. Interviews were thematically analysed. Based on the results, 30 key statements were listed and returned to participants for a ranking of their relevance. The main themes extracted are (1) available resources, (2) patient-related factors, (3) qualification and (4) preparation of and cooperation with the paediatric setting. In conclusion, it became evident that successful transition faces multiple obstacles. At the least, it asks for time, staff, and money. Rigid structures in health care leave little room for addressing the specific needs of this small group of patients. Transition becomes a topic one wants to and is able to afford.
The Role of the Versius Surgical Robotic System in the Paediatric Population
Authors: Brownlee Ewan M. , Slack Mark
Published: May 2022

The uptake of robot-assisted surgery has continuously grown since its advent in the 1990s. While robot-assisted surgery is well-established in adult surgery, the rate of uptake in paediatric surgical centres has been slower. The advantages of a robot-assisted system, such as improved visibility, dexterity, and ergonomics, could make it a superior choice over the traditional laparoscopic approach. However, its implementation in the paediatric surgery arena has been limited primarily due to the unavailability of appropriately sized instruments as per paediatric body habitus, therefore, requiring more technologically advanced systems. The Versius surgical robotic system is a new modular platform that offers several benefits such as articulated instruments which pass through conventional 5 mm ports, compact arms for easier manoeuvrability and patient access, the ability to mimic conventional port placements, and adaptive machine learning concepts. Prior to its introduction to paediatric surgery, it needs to go through a careful pre-clinical and clinical research program.
Perception of Changing Habits among Italian Children and Adolescents during COVID-19 Quarantine: An Epidemiological Study
Authors: Bassi Giulia , Mancinelli Elisa , Boldrini Bianca , Mondini Giada , Ferruzza Emilia , Di Riso Daniela , Salcuni Silvia
Published: May 2022

We used an epidemiological study to explore the perception of change in several psychosocial dimensions during the COVID-19 quarantine. We focused on emotions, use of social network sites (SNSs), family life, important relationships, body functions, and school life. Using snowball recruitment, N = 1047 Italian children, pre-adolescents, and adolescents (M = 13.74 ± 3.59) were selected to complete ad hoc online questionnaires. A differential semantic inventory plot was prepared to analyze the emotional experience of children, pre-adolescents, and adolescents during quarantine compared to the pre-quarantine period. The Kruskal–Wallis test was run to assess gender and age differences in emotions experienced, habitual SNS use, and the experience of attending classes remotely. A post hoc Wilcoxon test was performed to compare such differences. Results showed that most of the sample (93.1%) attended classes using technological devices and reported missing their classmates very much (59.3%). Adolescents experienced more negative emotions (M = 3.69 ± 1.33) than pre-adolescents (M = 4.64 ± 1.32), who experienced negative emotions more than children (M = 5.11 ± 1.24). Females were more prone to experience negative emotions compared to males. Adolescents were also the most prolific SNS users (78.1%), particularly female adolescents. Overall, these finding highlight the necessity of preserving the emotional state and relational well-being of youth in these developmental phases by considering their school and social lives.
Differences in Cognitive and Non-Cognitive Results between Only-Child and Non-Only-Child Children: Analysis of Propensity Scores Based on Large-Scale Assessment
Authors: Zhao Dexuan , He Zhuang , Tian Yi , Liu Hongyun
Published: May 2022

Based on the data of 3561 fifth-grade and 4062 eighth-grade students from the Beijing Assessment of Educational Quality in China, the present study used a propensity-value matching model to scientifically analyze only-child and non-only-child children in primary and secondary schools. Female differences in cognitive outcomes (linguistic performance) and non-cognitive outcomes (teacher-student relationships, peer relationships, and emotional management) were also evaluated. The results of the study were as follows. First, fifth-grade only-child students had a higher linguistic performance compared to that of their non-only-child counterparts, and the same result was found for eighth-grade students. Second, fifth- and eighth-grade only-child students had good teacher-student relationships that were not significantly different from those of their non-only-child counterparts. Third-, fifth-, and eighth-grade only-child students had significantly better peer relationships and emotional management compared to these parameters in their non-only-child counterparts.
Neonatal Abusive Head Trauma without External Injuries: Suspicion Improves Diagnosis
Authors: Yoon Seokwon , Lee Juyoung , Jun Yong Hoon , Jeon Ga Won
Published: May 2022

The term “shaken baby syndrome” has been replaced by “abusive head trauma (AHT)” based on the mechanism of injury. The reported mortality rate of AHT ranges from 10% to 30%. Up to two-thirds of survivors suffer from serious long-term disabilities. Thus, an expeditious and accurate diagnosis is crucial to prevent further abuse that might result in death or serious disabilities. It remains a challenge for physicians to diagnose AHT when parents do not give a history of trauma in preverbal infants without any external signs. Here, we report a case of a 14-day-old boy who presented with a febrile convulsion without evident external injuries nor history of trauma according to his parents. He was diagnosed with AHT based on MRI findings of subacute subdural hemorrhage, multiple cortical hemorrhages, cerebral edema, and diffuse axonal injury. In conclusion, health care providers should keep in mind that the history of trauma provided by the parents or caregivers might not always be true and that reasonable suspicion of abuse is the most important in the diagnosis of AHT, although neuroimaging plays a pivotal role. Reasonable suspicion of AHT in combination with a thorough physical examination, neuroimaging, and skilled neuroradiologist can improve diagnosis and help victims in a timely manner.
Advances in the Prenatal Management of Fetal Cardiac Disease
Authors: Sharma Chetan , Burns Joseph , Joshi Krittika , Gupta Monesha , Singh Harinder , Agarwal Arpit
Published: May 2022

Advances in the field have improved the prenatal management of cardiovascular diseases over the past few decades; however, there remains considerable challenges in the approach towards patient selection as well as the applicability of available therapies. This review aims to discuss the current knowledge, outcomes and challenges for prenatal intervention for congenital heart disease.
Inclusive Culture in Compulsory Education Centers: Values, Participation and Teachers’ Perceptions
Authors: Valle-Flórez Rosa-Eva , de Caso Fuertes Ana María , Baelo Roberto , Marcos-Santiago Rosario
Published: May 2022

This article explores teachers’ perceptions concerning educational inclusion as part of an inclusive culture. The study focuses on compulsory education from the teachers’ point of view. We used three factors indicated in the “Index of Inclusion”: inclusive values, degree of participation in the educational community, and the teachers’ perceptions of the educational response offered to SEN students. To comply with the proposed objective, we explored nine variables to understand their influence on the attitudes of teachers and other professionals towards educational inclusion. These variables were gender, age, teaching seniority, educational stage, professional profile, type of center, geographic location of the center, years of experience and characteristics of SEN students, as well as the training received to meet the needs of all students. We found significant differences in the variables of age, educational stage, student characteristics, and training received, and recommendations are provided to address the needs detected.