bluuebunny/crossref_metadata_2025 huggingface dataset:
The type 1 diabetes gene TYK2 regulates β-cell development and its responses to interferon-α
Authors: Chandra Vikash , Ibrahim Hazem , Halliez Clémentine , Prasad Rashmi , Vecchio Federica , Dwivedi Om Prakash , Kvist Jouni , Balboa Diego , Saarimäki-Vire Jonna , Montaser Hossam , Barsby Tom , Lithovius Väinö , Artner Isabella , Gopalakrishnan Swetha , Groop Leif , Mallone Roberto , Eizirik Decio L. , Otonkoski Timo
Published: February 2022

Type 1 diabetes (T1D) is an autoimmune disease that results in the destruction of insulin producing pancreatic β-cells. One of the genes associated with T1D is TYK2, which encodes a Janus kinase with critical roles in type-Ι interferon (IFN) mediated intracellular signaling. To study the role of TYK2 in human pancreatic β-cell development and response to IFNα, we generated TYK2 knockout human iPSCs and directed them into the pancreatic endocrine lineage. Here we show that loss of TYK2 compromised the emergence of endocrine precursors by regulating KRAS expression while mature stem cell-islets (SC-islets) function was not affected. In the maturing SC-islets, the loss or inhibition of TYK2 prevented IFNα-induced antigen processing and presentation, including MHC Class Ι expression in pancreatic endocrine and progenitor cells. Furthermore, in a CD8+ cytotoxic T-cell co-culture model, the survival of β-cells was enhanced by a selective TYK2 inhibitor. These results identify an unsuspected role for TYK2 on β-cell development and support TYK2 inhibition in adult β-cells as a potent therapeutic target to halt T1D progression.
Redifferentiated cardiomyocytes retain residual dedifferentiation signatures and are protected against ischaemic injury
Authors: Shakked Avraham , Petrover Zachary , Aharonov Alla , Ghiringhelli Matteo , Umansky Kfir-Baruch , Nguyen Phong Dang , Kain David , Elkahal Jacob , Divinsky Yalin , Miyara Shoval , Friedlander Gilgi , Savidor Alon , Zhang Lingling , Perez Dahlia , Kastan Nathaniel , Lendengolts Daria , Levin Yishai , Bakkers Jeroen , Gepstein Lior , Tzahor Eldad
Published: February 2022

Cardiomyocyte renewal by dedifferentiation and proliferation has fueled the field of regenerative cardiology in recent years, while the reverse process of redifferentiation remains largely unexplored. Redifferentiation is characterised by the restoration of function that is lost during dedifferentiation and is key to the healing process following injury. Previously, we showed that ERBB2-mediated heart regeneration has these two distinct phases: dedifferentiation, followed by redifferentiation. Here, using temporal RNAseq and proteomics, we survey the landscape of the dedifferentiation-redifferentiation process in the adult mouse heart. We find well characterised dedifferentiation pathways, such as reduced oxphos, increased proliferation and increased EMT-like features, largely return to normal, though elements of residual dedifferentiation remain, even after contractile function is restored. These hearts appeared rejuvenated and showed robust resistance to ischaemic injury. We find that redifferentiation is driven by negative feedback signalling, notably through LATS1/2 Hippo pathway activity. Disabling LATS1/2 in dedifferentiated cardiomyocytes augments dedifferentiation in vitro and prevents redifferentiation in vivo. Taken together, our data reveal the non-trivial nature of redifferentiation, whereby elements of dedifferentiation linger in a surprisingly beneficial manner. This cycle of dedifferentiation-redifferentiation protects against future insult, in what could become a novel prophylactic treatment against ischemic heart disease for at-risk patients.
Cell cycle-dependent binding between Cyclin B1 and Cdk1 revealed by time-resolved Fluorescence Correlation Spectroscopy
Authors: Barbiero Martina , Cirillo Luca , Veerapathiran Sapthaswaran , Coates Catherine , Ruffilli Camilla , Pines Jonathon
Published: February 2022

Measuring the dynamics with which the regulatory complexes of the cell cycle machinery assemble and disassemble is a crucial barrier to our understanding that until now has been difficult to address. This considerable gap in our understanding is due to the difficulty of reconciling biochemical assays with single cell-based techniques, but recent advances in microscopy and gene editing techniques now enable the measurement of protein-protein interaction kinetics in living cells.

Here, we apply Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Cross-Correlation Spectroscopy (FCCS) to study the dynamics of the cell cycle machinery, beginning with Cyclin B1 and its binding to its partner kinase Cdk1 that together form the major mitotic kinase. Although Cyclin B1 and Cdk1 are known to bind with high affinity, our results reveal that in living cells there is a pool of Cyclin B1 that is not bound to Cdk1. Furthermore, we provide evidence that the affinity of Cyclin B1 for Cdk1 increases during the cell cycle, indicating that the assembly of the complex is a regulated step. Our work lays the groundwork for studying the kinetics of protein complex assembly and disassembly during the cell cycle in living cells.
Lysine acetylation regulates antibiotic resistance in Escherichia coli
Authors: Fang Zuye , Lai Fubin , Cao Kun , Zhang Ziyuan , Cao Linlin , Liu Shiqin , Duan Yufeng , Yin Xingfeng , Ge Ruiguang , He Qing-Yu , Sun Xuesong
Published: February 2022

Antibiotic resistance is increasingly becoming a serious challenge to public health. The regulation of metabolism by post-translational modifications (PTMs) has been widely studied; however, the comprehensive mechanism underlying the regulation of acetylation in bacterial resistance against antibiotics is unknown. Herein, with Escherichia coli as the model, we performed quantitative analysis of the acetylated proteome of wild-type sensitive strain (WT) and ampicillin- (Re-Amp), kanamycin- (Re-Kan), and polymyxin B-resistant (Re-Pol) strains. Based on bioinformatics analysis combined with biochemical validations, we found that a common regulatory mechanism exists between the different resistant strains. Acetylation negatively regulates bacterial metabolism to maintain antibiotic resistance, but positively regulates bacterial motility. Further analyses revealed that key enzymes in various metabolic pathways were differentially acetylated. Particularly, pyruvate kinase (PykF), a key glycolytic enzyme regulating bacterial metabolism, and its acetylated form were highly expressed in the three resistant types and were identified as reversibly acetylated by the deacetylase CobB and the acetyl-transferase PatZ, and also could be acetylated by non-enzyme AcP in vitro. Further, the deacetylation of Lys413 of PykF increased the enzyme activity by changing the conformation of ATP binding site of PykF, resulting in an increase in energy production, which in turn increased the sensitivity of drug-resistant strains to antibiotics. This study provides novel insights for understanding bacterial resistance and lays the foundation for future research on regulation of acetylation in antibiotic-resistant strains.

Importance

The misuse of antibiotics has resulted in an emergence of a large number of antibiotic-resistant strains, which seriously threaten human health. Bacterial metabolism is tightly controlled by protein post-translational modifications, especially acetylation. However, the comprehensive mechanism underlying regulation of acetylation in bacterial resistance remains unexplored. Here, acetylation was found to positively regulate bacterial motility and negatively regulate energy metabolism, which was common in all the different antibiotic-resistant strains. Moreover, the acetylation and deacetylation process of PykF was uncovered, and deacetylation of the Lys 413 of PykF was found to contribute to bacterial sensitivity to antibiotics. This study provides a new direction for research on development of bacterial resistance through post-translational modifications and provides a theoretical basis for the development of antibacterial drugs.
Population structure and genetic connectivity of the scalloped hammerhead shark (Sphyrna lewini) across nursery grounds from the Eastern Tropical Pacific: implications for management and conservation
Authors: Elizondo-Sancho Mariana , Rodríguez-Arriatti Yehudi , Albertazzi Federico J. , Bonilla-Salazar Adrián , Arauz Daniel , Arauz Randall , Areano Elisa , Avalos-Castillo Cristopher G. , Brenes Óscar , Chávez Elpis J. , Dominici-Arosemena Arturo , Espinoza Mario , Heidemeyer Maike , Tavares Rafael , Hernández Sebastián
Published: February 2022

Defining demographically independent units and understanding gene flow between them is essential for managing and conserving exploited populations. The scalloped hammerhead shark, Sphyrna lewini, is a coastal semi-oceanic species found worldwide in tropical and subtropical waters. Pregnant females give birth in shallow coastal estuarine habitats that serve as nursery grounds for neonates and small juveniles, and adults move offshore and become highly migratory. We evaluated the population structure and connectivity of S. lewini in coastal areas across the Eastern Tropical Pacific (ETP) using both sequences of the mitochondrial DNA control region (mtCR) and nuclear-encoded microsatellite loci. The mtCR defined two genetically discrete geographic groups: the Mexican Pacific and the central-southern Eastern Tropical Pacific (Guatemala, Costa Rica, Panamá, and Colombia). Overall, the mtCR data showed low levels of haplotype diversity ranged from 0.000 to 0.608, while nucleotide diversity ranged from 0.000 to 0.0015. A more fine-grade population structure analysis was detected using microsatellite loci where Guatemala, Costa Rica, and Panamá differed significantly. Genetic diversity analysis with nuclear markers revealed an observed heterozygosity ranging from 0.68 to 0.71 and an allelic richness from 5.89 to 7.00. Relatedness analysis revealed that individuals within nursery areas were more closely related than expected by chance, suggesting that S. lewini may exhibit reproductive philopatric behaviour within the ETP. Findings of at least two different management units, and evidence of philopatric behaviour call for intensive conservation actions for this critically endangered species in the ETP.
Development of a physiological insulin resistance model in human stem cell-derived adipocytes
Authors: Friesen Max , Khalil Andrew S. , Barrasa M. Inmaculada , Jeppesen Jacob F. , Mooney David J. , Jaenisch Rudolf
Published: February 2022

Adipocytes are key regulatory cells of human metabolism, and their dysfunction in insulin signaling is central to metabolic diseases such as type II diabetes mellitus (T2D). However, the progression of insulin resistance that leads to T2D is still poorly understood. This limited understanding is due, in part, to the dearth of suitable models of insulin signaling in human adipocytes. Traditionally, in vitro adipocyte models fail to recapitulate in vivo insulin signaling, possibly due to exposure to supraphysiological nutrient and hormone conditions. Here, we have developed a sensitization protocol for human pluripotent stem cell-derived adipocytes that uses physiologically relevant nutrient conditions to produce a potent signaling response comparable to in vivo adipocytes. After systematically optimizing conditions, this protocol allows for robust insulin-stimulated glucose uptake and transcriptional insulin response. Furthermore, exposure of these sensitized adipocytes to physiologically relevant hyperinsulinemic conditions dampens insulin-stimulated glucose uptake and dysregulates transcription of insulin-responsive genes. Overall, this sensitization methodology provides a novel platform for the mechanistic study of insulin signaling and resistance using human pluripotent stem cell-derived adipocytes.

Teaser

A new protocol to generate hPSC-adipocytes that respond to physiological insulin levels and can model diabetes.
Evolutionary changes in the chromatin landscape contribute to reorganization of a developmental gene network during rapid life history evolution in sea urchins
Authors: Davidson Phillip L. , Byrne Maria , Wray Gregory A.
Published: February 2022

Chromatin configuration is highly dynamic during embryonic development in animals, exerting an important point of control in transcriptional regulation. Yet there exists remarkably little information about the role of evolutionary changes in chromatin configuration to the evolution of gene expression and organismal traits. Genome-wide assays of chromatin configuration, coupled with whole-genome alignments, can help address this gap in knowledge in several ways. In this study we present a comparative analysis of regulatory element sequences and accessibility throughout embryogenesis in three sea urchin species with divergent life histories: a lecithotroph Heliocidaris erythrogramma, a closely related planktotroph H. tuberculata, and a distantly related planktotroph Lytechinus variegatus. We identified distinct epigenetic and mutational signatures of evolutionary modifications to the function of putative cis-regulatory elements in H. erythrogramma that have accumulated non-uniformly throughout the genome, suggesting selection, rather than drift, underlies many modifications associated with the derived life history. Specifically, regulatory elements composing the sea urchin developmental gene regulatory network are enriched for signatures of positive selection and accessibility changes which may function to alter binding affinity and access of developmental transcription factors to these sites. Furthermore, regulatory element changes often correlate with divergent expression patterns of genes involved in cell type specification, morphogenesis, and development of other derived traits, suggesting these evolutionary modifications have been consequential for phenotypic evolution in H. erythrogramma. Collectively, our results demonstrate that selective pressures imposed by changes in developmental life history rapidly reshape the cis-regulatory landscape of core developmental genes to generate novel traits and embryonic programs.
A framework to score the effects of structural variants in health and disease
Authors: Kleinert Philip , Kircher Martin
Published: February 2022

Although technological advances improved the identification of structural variants (SVs) in the human genome, their interpretation remains challenging. Several methods utilize individual mechanistic principles like the deletion of coding sequence or 3D genome architecture disruptions. However, a comprehensive tool using the broad spectrum of available annotations is missing. Here, we describe CADD-SV, a method to retrieve and integrate a wide set of annotations to predict the effects of SVs. Previously, supervised learning approaches were limited due to a small number and biased set of annotated pathogenic or benign SVs. We overcome this problem by using a surrogate training objective, the Combined Annotation Dependent Depletion (CADD) of functional variants. We use human- and chimpanzee-derived SVs as proxy-neutral and contrast them with matched simulated variants as proxy-deleterious, an approach that has proven powerful for short sequence variants. Our tool computes summary statistics over diverse variant annotations and uses random forest models to prioritize deleterious structural variants. The resulting CADD-SV scores correlate with known pathogenic and rare population variants. We further show that we can prioritize somatic cancer variants as well as noncoding variants known to affect gene expression. We provide a website and offline-scoring tool for easy application of CADD-SV.
Understanding the purchase intentions for organic vegetables across EU: a proposal to extend the TPB model
Authors: Loera Barbara , Murphy Blain , Fedi Angela , Martini Mara , Tecco Nadia , Dean Moira
Published: February 2022

Purpose

The study aims to propose a systematic and innovative model of purchase intention development that integrates Theory of Planned Behaviour (TPB) with its main extensions and clarifies the logical status of the variables involved and the structure of the causal path.

Design/methodology/approach

The TPB is the most useful predictive model of purchase intentions, which can be viewed as the product of various psychological determinants. Previous works have proposed extensions of the TPB model to selectively include knowledge, trust or social norms, but an integrated proposal has not yet been formulated. Based on a survey in four European countries (Germany, Italy, Poland and the UK; N = 1,035), this study tests the process of organic vegetable purchase intention development using a structural equation model (SEM). This comprises part of the measurement of latent variables and part of the analysis of dependency relationships (MLR estimation method).

Findings

The results show that purchase intention for organic vegetables is primarily dependent on positive moral attitude (PoMA) towards such consumption. The inclusion of PoMA reduces the effect of attitude toward buying organic vegetables, but the effects of social norms, past behaviour and perceived behavioural control remain significant.

Originality/value

This study proposes an innovative model to explain purchase intention for organic vegetables that incorporates the key current extensions of the TPB model (knowledge, trust and PoMA) into an integrated causal pathway. Understanding the relationships between the antecedents of purchase intention provides relevant information on “what” needs to be improved and “where” interventions are needed to steer consumers towards organic food.
Local food: a constellation of perceived proximity
Authors: Chicoine Marilyne , Rodier Francine , Durif Fabien
Published: February 2022

Purpose

The purpose of this study is to explore the definition of local food through the concept of perceived proximity in order to improve the understanding of food locality and to propose a new framework for analysis.

Design/methodology/approach

The paper presents an exploratory research through 32 semi-structured interviews with six agri-food industry stakeholders carried out in Quebec, Canada. Thematic analysis is used to identify the main dimensions of the proximity of a local food. A conceptual framework based on the results is presented.

Findings

The results suggest that local food can be defined according to nine dimensions of proximity: geographic, process, price, identity, relational, functional, cultural, access and experiential.

Originality/value

This study allows the concept of local food to be broken down into a constellation of perceived proximities and expands the understanding of the differences in the perception of food locality.
On the application of the GS4-1 framework for fluid dynamics and adaptive time-stepping via a universal A-posteriori error estimator
Authors: Wang Yazhou , Xie Ningning , Yin Likun , Zhang Tong , Zhang Xuelin , Mei Shengwei , Xue Xiaodai , Tamma Kumar
Published: February 2022

Purpose

The purpose of this paper is to describe a novel universal error estimator and the adaptive time-stepping process in the generalized single-step single-solve (GS4-1) computational framework, applied for the fluid dynamics with illustrations to incompressible Navier–Stokes equations.

Design/methodology/approach

The proposed error estimator is universal and versatile that it works for the entire subsets of the GS4-1 framework, encompassing the nondissipative Crank–Nicolson method, the most dissipative backward differential formula and anything in between. It is new and novel that the cumbersome design work of error estimation for specific time integration algorithms can be avoided. Regarding the numerical implementation, the local error estimation has a compact representation that it is determined by the time derivative variables at four successive time levels and only involves vector operations, which is simple for numerical implementation. Additionally, the adaptive time-stepping is further illustrated by the proposed error estimator and is used to solve the benchmark problems of lid-driven cavity and flow past a cylinder.

Findings

The proposed computational procedure is capable of eliminating the nonphysical oscillations in GS4-1(1,1)/Crank–Nicolson method; being CPU-efficient in both dissipative and nondissipative schemes with better solution accuracy; and detecting the complex physics and hence selecting a suitable time step according to the user-defined error threshold.

Originality/value

To the best of the authors’ knowledge, for the first time, this study applies the general purpose GS4-1 family of time integration algorithms for transient simulations of incompressible Navier–Stokes equations in fluid dynamics with constant and adaptive time steps via a novel and universal error estimator. The proposed computational framework is simple for numerical implementation and the time step selection based on the proposed error estimation is efficient, benefiting to the computational expense for transient simulations.
Renewable energy and CO2 emissions: the economics and geopolitical implications, experiences from the BRICS nations
Authors: Ghosh Sudeshna
Published: February 2022

Purpose

The purpose of this study is to examine how renewable energy consumption moderates the relationship between inequality and carbon dioxide (CO2) emissions for Brazil, Russia, India, China and South Africa (BRICS). The nexus between energy use and geopolitical tensions has also been explored.

Design/methodology/approach

This study has used distinctive data sets from 1990 to 2018 to explore the interconnections on emission, energy use, inequality and geopolitics. To do away with the difficulties related to heterogeneity and cross-sectional dependence (CD), this paper uses recent estimation methods that are robust to panel heterogeneity and CD.

Findings

The results of the panel augmented mean group (AMG) estimation and common correlated effects mean group (CCEMG) estimation verify the environmental Kuznets curve. The findings show that a 1% rise in Gini inequality leads to a 0.24% rise in the CO2 emission (AMG) method and a 0.17% rise in emissions CCEMG (method). As far as the moderating impact of renewable energy upon Gini measure of inequality is concerned, it is −0.10 AMG and CCEMG methods of estimation, respectively. However, the moderating impact of renewable energy on the geopolitical index leads to a mitigating impact on CO2 emissions, 0.55% decline in AMG method.

Originality/value

This research makes a distinctive contribution by investigating for the first time to the best of the authors’ knowledge the main pillars of sustainable ecological development in the context of the BRICS nations.
Employing machine learning for capturing COVID-19 consumer sentiments from six countries: a methodological illustration
Authors: Schlegelmilch Bodo B. , Sharma Kirti , Garg Sambbhav
Published: February 2022

Purpose

This paper aims to illustrate the scope and challenges of using computer-aided content analysis in international marketing with the aim to capture consumer sentiments about COVID-19 from multi-lingual tweets.

Design/methodology/approach

The study is based on some 35 million original COVID-19-related tweets. The study methodology illustrates the use of supervised machine learning and artificial neural network techniques to conduct extensive information extraction.

Findings

The authors identified more than two million tweets from six countries and categorized them into PESTEL (i.e. Political, Economic, Social, Technological, Environmental and Legal) dimensions. The extracted consumer sentiments and associated emotions show substantial differences across countries. Our analyses highlight opportunities and challenges inherent in using multi-lingual online sentiment analysis in international marketing. Based on these insights, several future research directions are proposed.

Originality/value

First, the authors contribute to methodology development in international marketing by providing a “use-case” for computer-aided text mining in a multi-lingual context. Second, the authors add to the knowledge on differences in COVID-19-related consumer sentiments in different countries. Third, the authors provide avenues for future research on the analysis of unstructured multi-media posts.
Strengths and shortcomings of Latin American participation in post-conflict Colombia
Authors: Hirst Monica E. , Dalponte Bruno
Published: February 2022

Purpose

The purpose of this paper is to analyse the characteristics of the Latin American participation in the United Nations Mission in Colombia (UNMC), looking into the contributions of regional actors to the peace process in the immediate post-conflict. Testimonies from Latin American actors involved in the demobilization, disarmament and reintegration process help identify their perceptions of strengths and shortcomings of the mission. This paper analyses both the enduring relevance of their individual/institutional trajectories and the adaptations needed to implement the Peace Agreement in the Colombian context.

Design/methodology/approach

This is an exploratory case study. Primary data collection relied on in-depth, semi-structured interviews, allowing the authors to tap into how the trajectories of those involved help explain continuities and innovations of the UNMC regarding previous UN missions.

Findings

This paper argues that cultural factors are central to understand how Latin Americans participated in the UNMC. The design of the Monitoring and Verification Mechanism and the inclusion of a strong gender agenda make the UNMC a salient case, both for scholars studying Latin American presence in peace processes and for those looking into peace building, more generally.

Originality/value

The fieldwork, conducted with military/security forces, UN officials and civilians, has made available a diverse spectrum of testimonies that provide crucial insights into “lessons learned”, contributing to tracing the trajectories of these actors and providing insights for the improvement of future political and peacebuilding multilateral missions.
How ethnic dissimilarity influences perceived organizational support and organizational citizenship behaviors?
Authors: Tan Luen Peng , Choong Yuen Onn , Yap Ching Seng , Choe Kum Lung , Rungruang Parisa , Li Zhen
Published: February 2022

Purpose

This study examines the mediating effect of self-efficacy between perceived organizational support (POS) and organizational citizenship behaviors (OCB) organization (OCBO); and POS and OCB individual (OCBI) in a cross-cultural context.

Design/methodology/approach

Data were collected from 207 full-time academics from the private universities in Malaysia and Mainland China using a questionnaire survey.

Findings

The results indicate that self-efficacy is a salient mediator linking POS with OCBI and OCBO. Additionally, ethnic dissimilarity is found to have a contextual influence on the research model as the results reveal that self-efficacy only mediates the relationship between POS and OCBO but not between POS and OCBI in a heterogeneous society. In contrast, self-efficacy is found to mediate the relationships between POS and OCBO and between POS and OCBI in a homogeneous society.

Originality/value

This study contributes to the literature by being one of the first studies that examine the relationship between self-efficacy and two dimensions of OCB in two different cultural contexts.
The need for new public policies to increase entrepreneurship and spur economic growth
Authors: Singh Robert P.
Published: February 2022

Purpose

The purpose of this viewpoint paper is to bring attention to the declining rate of entrepreneurship, its effect on the broader economy, the need for public policy solutions and to offer several such solutions. Government intervention is needed to address the stagnation trends discussed throughout the paper. It is up to academic scholars, researchers and think tanks to make government leaders aware of the trends and possible solutions.

Design/methodology/approach

A literature review was performed and the need to increase entrepreneurship through public policies was discussed.

Findings

The current ten-year economic expansion is suffering from secular stagnation as a result of diminishing new venture creation and slower adoption of technological innovations. Economic policies are heavily tilted toward large, established firms even as new entrepreneurial ventures have greater impact on job creation. The importance of using public policies to spur economic growth through greater opportunity-based entrepreneurship is discussed. Unique tax policy changes are suggested as well as a proposed national business plan competition.

Originality/value

This paper makes a contribution to the entrepreneurship and public policy literature by discussing how the declining rate of entrepreneurship and slower adoption of new technology are related to the relatively tepid pace of economic expansion seen over the last decade in the US. The proposed policies focus on increasing new ventures based on opportunity-based entrepreneurship, and it is hoped that this will help in the development of other policies and spur new lines of research and knowledge that lead to increased economic growth.
Finance journal rankings: a paper affiliation methodology
Authors: Docampo Domingo , Safón Vicente
Published: February 2022

Purpose

In this paper, the authors use a new methodology, called paper affiliation index, to create finance journal ranking using expert judgment and research impact, both of which are based on secondary, objective measures, thus making it possible to produce lists every year without human manipulation at virtually no cost.

Design/methodology/approach

Bibliometrics. Python implementation.

Findings

A new ranking with 65 finance journals.

Research limitations/implications

This procedure helps to reduce bias and to deal with known problems associated with current methodologies. The data used in the methodology comes from public sources; the procedure is therefore easily replicable. This methodology is not subject-dependent and thus can be transferred to other realms of knowledge. Once the bibliometric institutional data has been gathered, the procedure is not computationally costly: a Python implementation of the algorithm executes the whole computation in a few seconds. Results seem to correct the pernicious Matthew effect which is so evident in citation-based methods.

Originality/value

The institutional classification created includes all institutions that have contributed papers to the field of finance. The procedure helps to reduce bias and to deal with known problems associated with current methodologies. The data used in the methodology comes from public sources, the procedure is therefore easily replicable. The methodology is not subject-dependent and thus can be transferred to other realms of knowledge. Once the bibliometric institutional data has been gathered, the procedure is not computationally costly.
Surmounting challenges and breaking barriers: will the cruise liners open up new frontiers in the new global village?
Authors: Ilori Matthew Olusoji , Ajagunna Ibrahim , Oluseyi Peter Olabisi , Lawal-Are Aderonke , Ogundipe Oluwatoyin Temitayo
Published: February 2022

Purpose

There are numerous pressures on the cruise tourism industry as in the recent past, many well-established cruise tourism locations have been turning tourists and ships away due to overtourism. It is argued here that now is the time to consider the introduction of new destinations.

Design/methodology/approach

The article is based on a literature review and discussions with experienced practitioners.

Findings

The cruise industry has continued to evolve and the numbers of people engaging in cruise tourism had been on the increase globally until the Covid-19 pandemic. Medium-sized and mega ships with state-of-the-art facilities were in regular use. Despite the fact that there was a boom, the need for an extension of cruise lines destinations has arisen as there are numerous nations with exciting locations waiting to be discovered. Some of the limitations of such destinations include difficulty of access for large mega ships and infrastructure. A way forward can be found though by engaging local smaller boats/ships to transfer tourists to local destinations.

Research limitations/implications

More work is needed to clarify issues such as (1) the readiness of proposed host communities to receive tourists; (2) terms and conditions of engagement of local boat companies; (3) cruising infrastructure development and (4) facilities upgrading that is compatible with international cruising standards.

Originality/value

This paper highlights some of the issues being faced by cruise companies in well-established tourism destinations. It also emphasizes the need for new, hitherto undiscovered locations to accommodate a likely expansion in demand in the post-Covid-19 era.
Young onset Parkinson’s disease: Biographical disruption as a repetitive process
Authors: Valasaki Maria
Published: February 2022

Young Onset Parkinson's Disease (YOPD) is a complex condition which raises issues of interpretation and understanding of individuals’ biographies. While interviewing seventeen people with YOPD from Greece, the issue of biographical disruption emerged. Parkinson's is a neurodegenerative disorder divided in five stages and has no permanent cure; based on this knowledge, individuals reconstruct their past and see their future. Under this perspective, unlike other chronic illnesses, in the case of Parkinson's disease interviewees have designated dimensions of disruption that are experienced due to continuous degeneration and while narrating their stories they designated disruptions even before diagnosis. In this article, biographical disruption is understood as a repetitive process and the latter is perceived as a continuous process caused by neurodegeneration; it never stops and is constructed through multiple dimensions: (a) bodily, (b) daily, (c) in stages and (d) biographical. These dimensions are interrelated and construct, both theoretically and analytically, the concept of disruption in daily life with a neurodegenerative disorder. Through the concept of repetitiveness, the complexity of living with PD is understood and also the need for deconstructing expected social roles.
Gastroesophageal reflux disease and paraneoplastic neurological syndrome associated with long‐term survival in limited stage small‐cell lung cancer
Authors: Ernani Vinicius , Du Lin , Ross Helen J. , Yi Joanne E. , Wampfler Jason A. , Schild Steven E. , Xie Hao , Swanson Karen L. , Tazelaar Henry D. , Yang Ping
Published: February 2022

Introduction

Patients with small‐cell lung cancer (SCLC) have a very poor prognosis. However, a subset of SCLC achieves long‐term survival. The objective of this study was to investigate factors and pattern of long‐term survival in patients with limited‐stage small cell lung cancer (LS‐SCLC) who achieved a complete response (CR) after chemoradiotherapy.

Patient and Methods

This was a single‐center retrospective study. The analysis of hazard ratio (HR) and 95% confidence interval (CI) was performed using Cox proportional hazards model. For pattern analysis, the date of recurrence was used as the endpoint. The nominal categorical variables were analyzed by the χ2 test. Survival was estimated using the Kaplan–Meier model, and the results were reported as the median and interquartile range.

Results

We identified 162 patients, median age was 64.7 (56.2–70.2) years, and 94 (58%) were females. Eighty‐one patients (50%) had recurrence during follow‐up. Gastroesophageal reflux disease (GERD) (HR, 0.65; 95% CI, 0.45–0.93; p = 0.016) and neurological paraneoplastic syndrome (PNS) (HR, 0.46; 95% CI, 0.29–0.72; p < 0.001) were independent factors associated with improved overall survival (OS). Patients with GERD had prolonged recurrence free survival (RFS) compared to patients without GERD (median, 29.1 months vs. 13.9 months, p < 0.001), whereas patients with neurological PNS had a reduced recurrence rate compared to those patients without neurological PNS (No. [%], 8 [20.5] vs. 73 [59.3], p < 0.001).

Conclusions

Patients with LS‐SCLC achieving a CR after chemoradiotherapy, GERD, and neurological PNS were associated with improved OS. GERD and neurological PNS were associated with longer RFS and lower recurrence rate, respectively.
Portable locomotion activity monitor (pLAM): A cost‐effective setup for robust activity tracking in small animals
Authors: Sondhi Yash , Jo Nicolas J. , Alpizar Britney , Markee Amanda , Dansby Hailey E. , Currea John Paul , Fabian Samuel T. , Ruiz Carlos , Barredo Elina , Allen Pablo , DeGennaro Matthew , Kawahara Akito Y. , Theobald Jamie C.
Published: February 2022

Characterising the frequency and timing of biological processes such as locomotion, eclosion or foraging, is often needed to get a complete picture of a species' ecology. Automated trackers are an invaluable tool for high‐throughput collection of activity data and have become more accurate and efficient with advances in computer vision and deep learning. However, tracking activity of small and fast flying animals remains a hurdle, especially in a field setting with variable light conditions. Commercial activity monitors can be expensive, closed source and generally limited to laboratory settings.

Here, we present a portable locomotion activity monitor (pLAM), a mobile activity detector to quantify small animal activity. Our setup uses inexpensive components, builds upon open‐source motion tracking software, and is easy to assemble and use in the field. It runs off‐grid, supports low‐light tracking with infrared lights and can implement arbitrary light cycle colours and brightnesses with programmable LEDs. We provide a user‐friendly guide to assembling pLAM hardware, accessing its pre‐configured software and guidelines for using it in other systems.

We benchmarked pLAM for insects under various laboratory and field conditions, then compared results to a commercial activity detector. They offer broadly similar activity measures, but our setup captures flight and bouts of motion that are often missed by beam breaking activity detection.

pLAM can automate laboratory and field monitoring of activity and timing in a wide range of biological processes, including circadian rhythm, eclosion and diapause timing, pollination and flower foraging, or pest feeding activity. This low cost and easy setup allows high‐throughput animal behaviour studies for basic and applied ecology and evolution research.
Multi‐scale habitat selection by a cryptic, critically endangered grassland bird—The Plains‐wanderer (Pedionomus torquatus): Implications for habitat management and conservation
Authors: Nugent Daniel T. , Baker‐Gabb David J. , Green Peter , Ostendorf Bertram , Dawlings Finella , Clarke Rohan H. , Morgan John W.
Published: February 2022

Our understanding of the habitat needs of grassland fauna is often incomplete because of their cryptic behaviour. This presents a barrier to identifying important habitat attributes, whether these change at different spatial scales, and how this informs management decisions. Here, we use a critically endangered bird, the Plains‐wanderer (Pedionomus torquatus, Pedionomidae), as an exemplar of the challenge of managing grasslands for cryptic species. Until now, almost all ecological knowledge of Plains‐wanderers has come from the detection of nocturnally roosting individuals and habitat assessments at fine‐scales that indicate open swards are preferred habitat. We GPS‐tracked 13 adult Plains‐wanderers to better understand diurnal habitat utilization in native grasslands of south‐eastern Australia. Using these data, we assessed whether Plains‐wanderers select for different habitat attributes during the day and night, and whether this varied according to spatial scale. At the fine‐scale (< 1 ha), daytime foraging occurred in denser swards than those of nocturnal roosting sites. At the patch‐scale (1–50 ha), Plains‐wanderers selected for denser vegetation, with higher grass and lichen cover, whilst avoiding areas where structure was impacted by a high exotic plant cover. Plains‐wanderers did not select habitat based on grassland type at the landscape‐scale (>100 ha). We demonstrate that Plains‐wanderers require grasslands with both open and denser swards to support foraging and roosting. In doing so, we address the biases associated with habitat assessments based on roost‐only locations and extend known habitat associations critical to the successful management of the species. Our findings highlight that a precautionary approach to the classification of habitat requirements is warranted when uncertainty around habitat use of cryptic grassland fauna exists.
Positioning kindness and care at the centre of health services: A case study of an informal health and development programme oriented to surviving well collectively
Authors: McKinnon Katharine
Published: February 2022

The mainstream development agenda highlights how important access to health care is for poorer regions of the world. In the area of maternal health, this is expressed in a concern to drive down rates of maternal morbidity and improve access to maternal health care services. While important, the focus on metrics misses the way that relations of care are fundamental to good health. This paper takes an example of a project which is offering a different approach to health and development in the resource scarce environment of Luang Prabang Province, in northern Laos. Here, a group of antipodean midwives has partnered with provincial health authorities to offer a midwifery training programme to health workers posted in remote rural health centres. Supported by the analytical tools of diverse economies, this paper explores how this programme centres relationality, collectivity and an ethic of kindness, and discusses the advantages of being relationship based, small and informal. The paper concludes that this training programme can be understood as an example of a community economy of care: based on global networks of care instead of formal development programmes built on global networks of bureaucracy.
Do bad apples do good deeds? The role of morality
Authors: Zhu Jinqiang , Xu Shiyong
Published: February 2022

Research on counterproductive work behaviours (CWBs) has predominantly adopted a victim‐centric approach to focus on deleterious effects on organisations while overlooking how displaying CWBs affects the employees themselves and their subsequent behaviours. Drawing on moral cleansing and moral licencing theories, we tested how and when employees’ CWBs influenced their subsequent organisational citizenship behaviours (OCBs) and whether OCBs affect CWBs. The results from an experimental study and a field study showed that CWBs were associated with guilt which, in turn, motivated employees to engage in more OCBs. In addition, employees with low moral relativism and high guilt‐repair proneness strengthened the link between CWBs, guilt and OCBs. We extend research on CWBs by switching focus from a victim‐centric perspective to a perpetrator‐centric perspective. In addition, we elucidate a specific mechanism and boundary conditions of moral cleansing theory.
Review: Cultural adaptations to psychosocial interventions for families with refugee/asylum‐seeker status in the United Kingdom – a systematic review
Authors: Taylor Alice , Radford Gillian , Calia Clara
Published: February 2022

Background

Young people with refugee or asylum‐seeker status (R/AS) often present with complex mental health needs, in the context of traumatic life experiences. Generic mental health services in the United Kingdom (UK) may be ill‐equipped to manage the unique experiences of these young people. Culturally adapted interventions (CAI) could provide a culturally sensitive approach to mental health support for refugee children experiencing difficult symptoms. A systematic review was conducted to determine the different types of cultural adaptation in the included studies, and to determine the efficacy of CAIs in comparison to generic treatment.

Methods

Systematic searches of eleven databases were completed in December 2020. Any psychosocial interventions conducted in the United Kingdom aimed at providing mental health support for refugee young people and families were included. This was to ensure the potential inclusion of all studies regardless of their adherence to the traditional framework of assessment and intervention in high‐income countries, for example randomised control trials.

Results

Eleven studies of varying methodology, participant group, intervention type and outcome measures were included in this review. Studies used a variety of cultural adaptations including surface‐level and deep‐level adaptations. Studies showed some support for the use of CAIs with young people with R/AS, with varying degrees of symptom reduction. It was not possible to compare the effectiveness of CAIs against ‘treatment‐as‐usual’, nor to determine the effectiveness of different CAI components.

Conclusions

Whilst there is evidence for the use of CAIs with R/AS young people, the heterogeneity between studies limits the generalisability of these results. The available research is not sufficient to provide conclusive evidence of the use of CAIs over ‘treatment‐as‐usual’. Research and clinical implications are highlighted. Future research could examine the most effective components of CAIs and aim to increase the evidence base of interventions for young people and families with R/AS.
Albumin to globulin ratio was associated with in‐stent restenosis and revascularization events after percutaneous coronary intervention
Authors: Zhang Rui , Tao Zaixiao , Gong Jing , Ji Zhenjun , Yang Mingming , Ma Genshan , Li Yongjun
Published: February 2022

In‐stent restenosis is a common complication after percutaneous coronary intervention (PCI) for coronary heart disease requiring revascularization. We performed a retrospective analysis to assess the value of inflammatory biomarker albumin to globulin ratio (AGR) in clinical prognosis of PCI. In total, 992 patients with coronary heart disease who underwent the first drug‐eluting stent implantation and re‐examination angiography in our hospital were enrolled in this study. The AGR was measured. At mean follow‐up of 11.2 ± 4 months, the in‐stent restenosis (ISR) and revascularization events (including target lesion revascularization, target vessel revascularization, and revascularization of de novo lesions) occurred in 127 and 284 patients, respectively. Compared with the non‐ISR or non‐event group, AGR was significantly lower in the ISR group and the events group. Beyond that, albumin was significantly lower, whereas urea nitrogen, glucose, and Gensini score, as well as the proportions of a history of diabetes and peripheral vascular diseases were significantly higher in the ISR group and the events group. Age, heart rate, white blood cell, neutrophils, lymphocyte, monocyte, and incidence of ischemic stroke were significantly higher in the events group. Multivariate Cox regression analysis showed that AGR was independently associated with ISR (p = 0.032) and events (p = 0.024). Besides, Kaplan‐Meier analysis indicated that the higher quartile of AGR had a lower rate of ISR (p = 0.038) and events (p ≤ 0.001). Finally, the receiver operating characteristic curve for AGR in diagnosing ISR and events indicated that the area under the curve were 0.56 and 0.57, respectively. Therefore, AGR is one of the most important factors that independently associate with the ISR and revascularization events after PCI.