bluuebunny/crossref_metadata_2025 huggingface dataset:
Alterations of the Adipo–Myokine Irisin in Sepsis and Septic Shock: Diagnostic and Prognostic Implications
Authors: Karampela Irene , Vallianou Natalia G. , Tsilingiris Dimitrios , Christodoulatos Gerasimos Socrates , Psallida Sotiria , Kounatidis Dimitris , Stratigou Theodora , Marinou Ioanna , Vogiatzakis Evaggelos , Dalamaga Maria
Published: February 2024

Irisin, a novel adipo-myokine with metabolic regulatory functions, exerts anti-inflammatory, antioxidant, and anti-apoptotic actions that may confer protection against sepsis-induced organ injury in experimental studies. Until now, only one human study has explored circulating irisin at sepsis onset. We aimed to examine serum irisin and its kinetics in critically ill patients with sepsis and septic shock with regard to sepsis severity and outcome. We enrolled 102 critically ill patients with sepsis or septic shock within 48 h of diagnosis and 102 age- and gender-matched healthy controls. Irisin was determined in serum upon enrollment in all participants and one week later in patients using an immunoenzymatic method. The outcome of sepsis was recorded 28 days after enrollment. At enrollment, circulating irisin was significantly lower in patients than controls (22.3 ± 6.8 μg/L vs. 28.1 ± 6.7 μg/L, p < 0.001), and increased significantly one week later (22.3 ± 6.8 μg/L vs. 26.6 ± 9.5 μg/L, p < 0.001). Irisin was significantly lower in patients who presented with septic shock than those with sepsis, and in non-survivors than survivors both at enrollment and one week later. However, kinetics of irisin did not differ between the groups (p > 0.05). Patients with higher circulating irisin during the first week of sepsis had a better outcome (p < 0.001). Lower irisin was independently associated with 28-day mortality (sepsis onset: HR 0.44, 95% C.I. 0.26–0.77, p = 0.004 and one week after: HR 0.37, 95% C.I. 0.23–0.58, p < 0.001). Irisin was negatively correlated with severity scores, metabolic, and inflammatory biomarkers. Circulating irisin decreases early in sepsis and is an independent predictor of 28-day mortality. Irisin may be a promising diagnostic and prognostic sepsis biomarker; nevertheless, larger studies are needed to explore its role in sepsis.
TRPV Channels in Osteoarthritis: A Comprehensive Review
Authors: Chen Changshun , Yang Fei , Chen Rongjin , Yang Chenhui , Xiao Hefang , Geng Bin , Xia Yayi
Published: February 2024

Osteoarthritis (OA) is a debilitating joint disorder that affects millions of people worldwide. Despite its prevalence, our understanding of the underlying mechanisms remains incomplete. In recent years, transient receptor potential vanilloid (TRPV) channels have emerged as key players in OA pathogenesis. This review provides an in-depth exploration of the role of the TRPV pathway in OA, encompassing its involvement in pain perception, inflammation, and mechanotransduction. Furthermore, we discuss the latest research findings, potential therapeutic strategies, and future directions in the field, shedding light on the multifaceted nature of TRPV channels in OA.
Pregnancy-Specific Beta-1-Glycoprotein 1 Increases HTR-8/SVneo Cell Migration through the Orai1/Akt Signaling Pathway
Authors: Wang Qunhua , Fang Yan , Li Yuan , Liu Huali , Zhu Maoni , Hu Xue , Zhou Jinzhuo , Deng Anqi , Shen Bing , Chen Hongbo
Published: February 2024

The impaired invasion ability of trophoblast cells is related to the occurrence of preeclampsia (PE). We previously found that pregnancy-specific beta-1-glycoprotein 1 (PSG1) levels were decreased in the serum of individuals with early-onset preeclampsia (EOPE). This study investigated the effect of PSG1 on Orai1-mediated store-operated calcium entry (SOCE) and the Akt signaling pathway in human trophoblast cell migration. An enzyme-linked immunosorbent assay (ELISA) was used to determine the level of PSG1 in the serum of pregnant women with EOPE. The effects of PSG1 on trophoblast proliferation and migration were examined using cell counting kit-8 (CCK8) and wound healing experiments, respectively. The expression levels of Orai1, Akt, and phosphorylated Akt (p-Akt) were determined through Western blotting. The results confirmed that the serum PSG1 levels were lower in EOPE women than in healthy pregnant women. The PSG1 treatment upregulated the protein expression of Orai1 and p-Akt. The selective inhibitor of Orai1 (MRS1845) weakened the migration-promoting effect mediated by PSG1 via suppressing the Akt signaling pathway. Our findings revealed one of the mechanisms possibly involved in EOPE pathophysiology, which was that downregulated PSG1 may reduce the Orai1/Akt signaling pathway, thereby inhibiting trophoblast migration. PSG1 may serve as a potential target for the treatment and diagnosis of EOPE.
TNF Superfamily and ILC2 Activation in Asthma
Authors: Matsuyama Takahiro , Salter Brittany Marie , Emami Fard Nahal , Machida Kentaro , Sehmi Roma
Published: February 2024

Eosinophilic asthma is the most prevalent and well-defined phenotype of asthma. Despite a majority of patients responding to corticosteroid therapy and T2 biologics, there remains a subset that have recurrent asthma exacerbations, highlighting a need for additional therapies to fully ameliorate airway eosinophilia. Group 2 innate lymphoid cells (ILC2) are considered key players in the pathogenesis of eosinophilic asthma through the production of copious amounts of type 2 cytokines, namely IL-5 and IL-13. ILC2 numbers are increased in the airways of asthmatics and with the greatest numbers of activated ILC2 detected in sputa from severe prednisone-dependent asthma with uncontrolled eosinophilia. Although epithelial-derived cytokines are important mediators of ILC2 activation, emerging evidence suggests that additional pathways stimulate ILC2 function. The tumor necrosis factor super family (TNFSF) and its receptors (TNFRSF) promote ILC2 activity. In this review, we discuss evidence supporting a relationship between ILC2 and TNFSF/TNFRSF axis in eosinophilic asthma and the role of this relationship in severe asthma with airway autoimmune responses.
Fibroblast Activation Protein (FAP)-Mediated Cleavage of Type III Collagen Reveals Serum Biomarker Potential in Non-Small Cell Lung Cancer and Spondyloarthritis
Authors: Pedersen Rasmus S. , Thorlacius-Ussing Jeppe , Raimondo Maria G. , Langholm Lasse L. , Schett Georg , Ramming Andreas , Karsdal Morten , Willumsen Nicholas
Published: February 2024

Fibroblast activation protein (FAP) is a known promoter of tumor development and is associated with poor clinical outcome for various cancer types. Being specifically expressed in pathological conditions including multiple types of fibrosis and cancers, FAP is an optimal target for diagnostics and treatment. Treatment strategies utilizing the unique proteolytic activity of FAP are emerging, thus emphasizing the importance of biomarkers to directly assess FAP activity. FAP is a type II transmembrane serine protease that has been shown to cleave collagens and other ECM components. In this study, we developed an ELISA assay (C3F) targeting a circulating type III collagen fragment derived from FAP cleavage to reflect FAP activity. We demonstrated that C3F was specific to the neoepitope of the cleavage site and that the fragment was generated through FAP cleavage of type III collagen. We measured C3F in serum from a cohort of patients with non-small cell lung cancer (NSCLC) (n = 109) matched to healthy subjects (n = 42) and a cohort of patients with spondyloarthritis (SpA) (n = 17) matched to healthy subjects (n = 19). We found that C3F was significantly elevated in patients with NSCLC and in patients with SpA compared to healthy controls (p < 0.0001 and p = 0.0015, respectively). These findings suggest that C3F is a promising non-invasive biomarker reflecting FAP activity, which may aid in understanding tumor heterogeneity and potentially FAP-targeted therapies.
New Insights into Chemoresistance Mediated by Mdm2 Inhibitors: The Benefits of Targeted Therapy over Common Cytostatics
Authors: Grigoreva Tatyana , Sagaidak Aleksandra , Novikova Daria , Tribulovich Vyacheslav
Published: February 2024

The inhibition of the Mdm2-p53 protein–protein interaction is a promising strategy for anticancer therapy. However, the problem of developing secondary chemoresistance in tumors treated with such drugs has not yet been sufficiently studied. In this work, we compared the properties of a drug-resistant cell line obtained during long-term cultivation in the presence of an Mdm2 inhibitor, Nutlin-3a, with a similarly obtained line insensitive to the cytostatic drug paclitaxel. We first confirmed the higher safety levels of Mdm2 inhibitors when compared with cytostatics in terms of the development of secondary chemoresistance. We showed that Nutlin-3a affects both the targeted p53-mediated cellular machinery and the universal ABC-mediated efflux mechanism. While both targeted and general defense mechanisms are activated by the Mdm2 inhibitor, it still increases the susceptibility of tumor cells to other drugs. The results obtained indicate that the risks of developing chemoresistance under the therapy with a targeted agent are fundamentally lower than during cytotoxic therapy.
Some Novel Therapies in Parkinson’s Disease: A Promising Path Forward or Not Yet? A Systematic Review of the Literature
Authors: Bougea Anastasia
Published: February 2024

In light of the unsuccessful traditional therapies for Parkinson’s disease (PD) overmany years, there is an unmet need for the development of novel therapies to alleviate the symptoms of PD retardation or halt the progression of the disease itself. This systematic review aims to critically update some of the most promising novel treatments including gene therapy, cell-based therapies, targeted drug delivery, and neuroprotective agents, focusing on their challenges, limitations and future directions in PD research. Gene therapy in PD is encouraging, with AAV-based approaches targeting neurotrophic factors, dopamine production, and neuronal circuits in animal and clinical trials. A promising approach to targeted drug delivery for PD involves the use of nanotechnology to create drug delivery vehicles that can traverse the blood–brain barrier and deliver medications specifically to the regions of the brain affected by PD. Neuroprotective agents are compounds that have the ability to protect neurons from degeneration and death, and they hold great promise for the evolution of disease-modifying treatments for PD. Magnetic field therapy is a promising non-invasive method that promotes neural plasticity in PD. The establishment of standardized protocols for animal and human studies, safety, ethical considerations, and cost-effectiveness are the major challenges for the future research of novel PD therapies. The development of novel therapies for PD represents a promising path toward to effective personalized disease-modifying treatments for PD.
Maneuvering Characteristics of Bilateral Amplitude–Asymmetric Flapping Motion Based on a Bat-Inspired Flexible Wing
Authors: Lilong Chuyi , Yu Yongliang
Published: February 2024

Flapping-wing micro air vehicles (FWMAVs) have gained much attention from researchers due to their exceptional performance at low Reynolds numbers. However, the limited understanding of active aerodynamic modulation in flying creatures has hindered their maneuverability from reaching that of their biological counterparts. In this article, experimental investigations were conducted to examine the effect of the bilateral amplitude asymmetry of flexible flapping wings. A reduced bionic model featuring bat-like wings is built, and a dimensionless number ΔΦ* is introduced to scale the degree of bilateral amplitude asymmetry in flapping motion. The experimental results suggest that the bilateral amplitude–asymmetric flapping motion primarily induces maneuvering control forces of coupling roll moment and yaw moment. Also, roll moment and yaw moment have a good linear relationship. To achieve more efficient maneuvers based on this asymmetric motion, it is advisable to maintain ΔΦ* within the range of 0 to 0.4. The magnitude of passive pitching deformation during the downstroke is significantly greater than that during the upstroke. The phase of the peak of the passive pitching angle advances with the increase in flapping amplitude, while the valleys lag. And the proportion of pronation and supination in passive pitching motion cannot be adjusted by changing the flapping amplitude. These findings have important practical relevance for regulating turning maneuvers based on amplitude asymmetry and help to understand the active aerodynamic modulation mechanism through asymmetric wing kinematics.
Short- and Long-Term Outcomes of an Adventure Therapy Programme on Borderline Personality Disorder: A Pragmatic Controlled Clinical Trial
Authors: Gabarda-Blasco Alba , Elias Aina , Mendo-Cullell Mariona , Arenas-Pijoan Laura , Forné Carles , Fernandez-Oñate David , Bossa Laura , Torrent Aurora , Gallart-Palau Xavier , Batalla Iolanda
Published: February 2024

Adventure Therapy (AT) is a therapeutic intervention utilizing the natural environment and adventure activities as tools for psychotherapeutic interventions. It has been demonstrated to be appropriate for the intervention of patients with borderline personality disorder (BPD). This study aims to evaluate the response to AT treatment compared with the response to treatment as usual (TAU), based on cognitive behavioural therapy, in the short and long term, assessing clinical, psychosocial, and functional outcomes; quality of life; and physical health levels. This study extends the sample of and is a follow-up to a pilot study published in 2021, with a sample of 30 patients in the AT group and 10 in the control group. It does not allow us to affirm that AT provides better outcomes than TAU, as the positive effects observed immediately after therapy seem to be attenuated in the long term. Therefore, the effectiveness of long-term psychotherapy did not show differences between AT and TAU therapies in the treatment of BPD patients. However, the effects of intangibles observed during therapy by professionals and patients were not reflected in the measurements collected. Therefore, we believe it is necessary to increase the programme duration, complement treatment with a specific physical health programme, assess results with more specific instruments, and/or move towards a qualitative methodology to measure perceived changes in clinical improvement. New studies are needed to evaluate the results of the proposed changes.
The Impact of the Need for Cognitive Closure on Attitudes toward Women as Managers and the Sequential Mediating Role of Belief in a Just World and Gender Essentialism
Authors: Baldner Conrad , Pierro Antonio
Published: February 2024

This research investigated the relation between the need for cognitive closure (i.e., a desire for epistemic certainty) and attitudes toward women as managers among men and women. In a cross-sectional study (total N = 241) collected in Italy, we found that need for cognitive closure, controlling for participants’ gender, was related to having more prejudice toward women leaders. Furthermore, the results revealed that the positive relation between the need for cognitive closure and negative attitudes toward women as managers was sequentially mediated by belief in a just world (i.e., the belief that people get what they deserve and deserve what they get and other people do not) and gender essentialism (i.e., the belief that women and men are distinctly, immutably, and naturally different, and thus have complementary skills to bring to the workplace). We suggest that men and women who are characterized by a need for cognitive closure are more sensitive to stereotypes of women as being incompatible with leadership roles. Either priming a low need for cognitive closure or providing contrary stereotypes could obviate the effect on beliefs in a just world and in gender essentialism that impedes progress towards greater gender equality in the workplace.
Prediction of the Unconfined Compressive Strength of Salinized Frozen Soil Based on Machine Learning
Authors: Zhao Huiwei , Bing Hui
Published: February 2024

Unconfined compressive strength (UCS) is an important parameter of rock and soil mechanical behavior in foundation engineering design and construction. In this study, salinized frozen soil is selected as the research object, and soil GDS tests, ultrasonic tests, and scanning electron microscopy (SEM) tests are conducted. Based on the classification method of the model parameters, 2 macroscopic parameters, 38 mesoscopic parameters, and 19 microscopic parameters are selected. A machine learning model is used to predict the strength of soil considering the three-level characteristic parameters. Four accuracy evaluation indicators are used to evaluate six machine learning models. The results show that the radial basis function (RBF) has the best UCS predictive performance for both the training and testing stages. In terms of acceptable accuracy and stability loss, through the analysis of the gray correlation and rough set of the three-level parameters, the total amount and proportion of parameters are optimized so that there are 2, 16, and 16 macro, meso, and micro parameters in a sequence, respectively. In the simulation of the aforementioned six machine learning models with the optimized parameters, the RBF still performs optimally. In addition, after parameter optimization, the sensitivity proportion of the third-level parameters is more reasonable. The RBF model with optimized parameters proved to be a more effective method for predicting soil UCS. This study improves the prediction ability of the UCS by classifying and optimizing the model parameters and provides a useful reference for future research on salty soil strength parameters in seasonally frozen regions.
Nano-Scale and Macro-Scale Characterizations of the Effects of Recycled Plastics on Asphalt Binder Properties
Authors: Al-Hosainat Ahmad , Nazzal Munir D. , Kaya Savas , Reza Toufiq
Published: February 2024

This paper summarizes the results of one of the first comprehensive laboratory studies that was conducted to evaluate the effects of adding different contents of recycled polyethylene terephthalate (rPETE) as a modifier to an asphalt binder on the rheological and mechanical properties of the modified binder as well as on the agglomeration behavior between the rPETE and asphalt binder at a multiscale level. The high-temperature and low-temperature performances of the modified binder were investigated at the macro-scale and compared with those of the unmodified binder using dynamic shear rheometer (DSR) and bending-beam rheometer (BBR) rheological tests, as well as asphalt binder cracking device (ABCD) testing. The nano-scale evaluation of the binder properties, including the surface roughness, bonding energy, and reduced modulus, was accomplished using atomic force microscopy (AFM). The results indicated that the addition of rPETE enhanced the high- and intermediate-temperature rheological properties of the modified PG 64-22 binder. The low-temperature rheological properties and resistance to cracking decreased slightly with increasing rPETE content in the asphalt binder. However, this reduction was not remarkable when adding 4%, 8%, and 10% rPETE contents. The asphalt binder modified with 4% rPETE had a low-temperature grade of −22, similar to that of the unmodified binder, indicating that 4% rPETE can be added to the binder to improve its high- and intermediate-temperature properties without reducing its resistance to low-temperature damage. The AFM tapping-mode results indicated that the inclusion of rPETE in the asphalt binder improved the stiffness properties of the modified binder as compared with those of the control asphalt binder. In addition, the rPETE-modified binders showed rougher surfaces than the control binder. The addition of rPETE to the binder increased the values of the reduced modulus and bonding energy compared with those of the control binder.
Improving Project Estimates at Completion through Progress-Based Performance Factors
Authors: Ottaviani Filippo Maria , De Marco Alberto , Narbaev Timur , Rebuglio Massimo
Published: February 2024

From a managerial perspective, project success hinges on estimates at completion as they allow tailoring response actions to cost and schedule overruns. While the literature is moving towards sophisticated approaches, standard methodologies, such as Earned-Value Management (EVM) and Earned Schedule (ES), are barely implemented in certain contexts. Therefore, it is necessary to improve performance forecasting without increasing its difficulty. The objective of this study was twofold. First, to guide modeling and implementing project progress within cost and to schedule Performance Factors (PFs). Second, to test several PFs utilized within EVM and ES formulae to forecast project cost and duration at completion. Progress indicators dynamically adjust the evaluation approach, shifting from neutral to conservative as the project progresses, either physically or temporally. This study compared the performance of the progress-based PFs against EVM and ES standard, combined, and average-based PFs on a dataset of 65 real construction projects, in both cost and duration forecasting. The results show that progress-based PFs provide more accurate, precise, and timely forecasts than other PFs. This study allows practitioners to select one or more of the proposed PFs, or even to develop one, following the guidelines provided, to reflect best their assumptions about the future course of project performance.
Epigenetic Mechanisms in Latent Epstein-Barr Virus Infection and Associated Cancers
Authors: Torne Atharva S. , Robertson Erle S.
Published: February 2024

The Epstein–Barr Virus (EBV) is a double-stranded DNA-based human tumor virus that was first isolated in 1964 from lymphoma biopsies. Since its initial discovery, EBV has been identified as a major contributor to numerous cancers and chronic autoimmune disorders. The virus is particularly efficient at infecting B-cells but can also infect epithelial cells, utilizing an array of epigenetic strategies to establish long-term latent infection. The association with histone modifications, alteration of DNA methylation patterns in host and viral genomes, and microRNA targeting of host cell factors are core epigenetic strategies that drive interactions between host and virus, which are necessary for viral persistence and progression of EBV-associated diseases. Therefore, understanding epigenetic regulation and its role in post-entry viral dynamics is an elusive area of EBV research. Here, we present current outlooks of EBV epigenetic regulation as it pertains to viral interactions with its host during latent infection and its propensity to induce tumorigenesis. We review the important epigenetic regulators of EBV latency and explore how the strategies involved during latent infection drive differential epigenetic profiles and host-virus interactions in EBV-associated cancers.
Correction: Soung et al. Therapeutic Potential of Chemically Modified MiR-489 in Triple-Negative Breast Cancers. Cancers 2020, 12, 2209
Authors: Soung Young Hwa , Chung Heesung , Yan Cecilia , Fesler Andrew , Kim Hyungjin , Oh Eok-Soo , Ju Jingfang , Chung Jun
Published: February 2024

In the original publication [...]
Lung Cancers: Parenchymal Biochemistry and Mechanics
Authors: Lecarpentier Yves , Tremblay Bruno , Locher Christèle , Schussler Olivier , Vallée Alexandre , Locher Christophe , Pho David
Published: February 2024

Parenchyma of pulmonary cancers acquires contractile properties that resemble those of muscles but presents some particularities. These non-muscle contractile tissues could be stimulated either electrically or chemically (KCl). They present the Frank–Starling mechanism, the Hill hyperbolic tension–velocity relationship, and the tridimensional time-independent tension–velocity–length relationship. Relaxation could be obtained by the inhibition of crossbridge molecular motors or by a decrease in the intracellular calcium concentration. They differ from muscles in that their kinetics are ultraslow as evidenced by their low shortening velocity and myosin ATPase activity. Contractility is generated by non-muscle myosin type II A and II B. The activation of the β-catenin/WNT pathway is accompanied by the high level of the non-muscle myosin observed in lung cancers.
Harnessing the Diversity of Burkholderia spp. Prophages for Therapeutic Potential
Authors: Nordstrom Hayley R. , Griffith Marissa P. , Rangachar Srinivasa Vatsala , Wallace Nathan R. , Li Anna , Cooper Vaughn S. , Shields Ryan K. , Van Tyne Daria
Published: February 2024

Burkholderia spp. are often resistant to antibiotics, and infections with these organisms are difficult to treat. A potential alternative treatment for Burkholderia spp. infections is bacteriophage (phage) therapy; however, it can be difficult to locate phages that target these bacteria. Prophages incorporated into the bacterial genome have been identified within Burkholderia spp. and may represent a source of useful phages for therapy. Here, we investigate whether prophages within Burkholderia spp. clinical isolates can kill conspecific and heterospecific isolates. Thirty-two Burkholderia spp. isolates were induced for prophage release, and harvested phages were tested for lytic activity against the same 32 isolates. Temperate phages were passaged and their host ranges were determined, resulting in four unique phages of prophage origin that showed different ranges of lytic activity. We also analyzed the prophage content of 35 Burkholderia spp. clinical isolate genomes and identified several prophages present in the genomes of multiple isolates of the same species. Finally, we observed that Burkholdera cenocepacia isolates were more phage-susceptible than Burkholderia multivorans isolates. Overall, our findings suggest that prophages present within Burkholderia spp. genomes are a potentially useful starting point for the isolation and development of novel phages for use in phage therapy.
Advanced Glycation End Products Upregulate CD40 in Human Retinal Endothelial and Müller Cells: Relevance to Diabetic Retinopathy
Authors: Portillo Jose-Andres C. , Pfaff Amelia , Vos Sarah , Weng Matthew , Nagaraj Ram H. , Subauste Carlos S.
Published: February 2024

CD40 induces pro-inflammatory responses in endothelial and Müller cells and is required for the development of diabetic retinopathy (DR). CD40 is upregulated in these cells in patients with DR. CD40 upregulation is a central feature of CD40-driven inflammatory disorders. What drives CD40 upregulation in the diabetic retina remains unknown. We examined the role of advanced glycation end products (AGEs) in CD40 upregulation in endothelial cells and Müller cells. Human endothelial cells and Müller cells were incubated with unmodified or methylglyoxal (MGO)-modified fibronectin. CD40 expression was assessed by flow cytometry. The expression of ICAM-1 and CCL2 was examined by flow cytometry or ELISA after stimulation with CD154 (CD40 ligand). The expression of carboxymethyl lysine (CML), fibronectin, and laminin as well as CD40 in endothelial and Müller cells from patients with DR was examined by confocal microscopy. Fibronectin modified by MGO upregulated CD40 in endothelial and Müller cells. CD40 upregulation was functionally relevant. MGO-modified fibronectin enhanced CD154-driven upregulation of ICAM-1 and CCL2 in endothelial and Müller cells. Increased CD40 expression in endothelial and Müller cells from patients with DR was associated with increased CML expression in fibronectin and laminin. These findings identify AGEs as inducers of CD40 upregulation in endothelial and Müller cells and enhancers of CD40-dependent pro-inflammatory responses. CD40 upregulation in these cells is associated with higher CML expression in fibronectin and laminin in patients with DR. This study revealed that CD40 and AGEs, two important drivers of DR, are interconnected.
The Role of β-Dystroglycan in Nuclear Dynamics
Authors: Cook Matthew , Stevenson Ben , Jacobs Laura A. , Leocadio Victoria Daniel , Cisneros Bulmaro , Hobbs Jamie K. , Stewart Colin L. , Winder Steve J.
Published: February 2024

Dystroglycan is a ubiquitously expressed heterodimeric cell-surface laminin receptor with roles in cell adhesion, signalling, and membrane stabilisation. More recently, the transmembrane β-subunit of dystroglycan has been shown to localise to both the nuclear envelope and the nucleoplasm. This has led to the hypothesis that dystroglycan may have a structural role at the nuclear envelope analogous to its role at the plasma membrane. The biochemical fraction of myoblast cells clearly supports the presence of dystroglycan in the nucleus. Deletion of the dystroglycan protein by disruption of the DAG1 locus using CRISPR/Cas9 leads to changes in nuclear size but not overall morphology; moreover, the Young’s modulus of dystroglycan-deleted nuclei, as determined by atomic force microscopy, is unaltered. Dystroglycan-disrupted myoblasts are also no more susceptible to nuclear stresses including chemical and mechanical, than normal myoblasts. Re-expression of dystroglycan in DAG1-disrupted myoblasts restores nuclear size without affecting other nuclear parameters.
Modulations of Homeostatic ACE2, CD147, GRP78 Pathways Correlate with Vascular and Endothelial Performance Markers during Pulmonary SARS-CoV-2 Infection
Authors: Nisa Annuurun , Kumar Ranjeet , Ramasamy Santhamani , Kolloli Afsal , Olejnik Judith , Jalloh Sallieu , Gummuluru Suryaram , Subbian Selvakumar , Bushkin Yuri
Published: February 2024

The pathologic consequences of Coronavirus Disease-2019 (COVID-19) include elevated inflammation and dysregulated vascular functions associated with thrombosis. In general, disruption of vascular homeostasis and ensuing prothrombotic events are driven by activated platelets, monocytes, and macrophages, which form aggregates (thrombi) attached to the endothelium lining of vessel walls. However, molecular pathways underpinning the pathological interactions between myeloid cells and endothelium during COVID-19 remain undefined. Here, we tested the hypothesis that modulations in the expression of cellular receptors angiotensin-converting enzyme 2 (ACE2), CD147, and glucose-regulated protein 78 (GRP78), which are involved in homeostasis and endothelial performance, are the hallmark responses induced by SARS-CoV-2 infection. Cultured macrophages and lungs of hamster model systems were used to test this hypothesis. The results indicate that while macrophages and endothelial cells are less likely to support SARS-CoV-2 proliferation, these cells may readily respond to inflammatory stimuli generated by the infected lung epithelium. SARS-CoV-2 induced modulations of tested cellular receptors correlated with corresponding changes in the mRNA expression of coagulation cascade regulators and endothelial integrity components in infected hamster lungs. Among these markers, tissue factor (TF) had the best correlation for prothrombotic events during SARS-CoV-2 infection. Furthermore, the single-molecule fluorescence in situ hybridization (smFISH) method alone was sufficient to determine the peak and resolution phases of SARS-CoV-2 infection and enabled screening for cellular markers co-expressed with the virus. These findings suggest possible molecular pathways for exploration of novel drugs capable of blocking the prothrombotic shift events that exacerbate COVID-19 pathophysiology and control the disease.
Ferroptosis-Related lncRNA to Predict the Clinical Outcomes and Molecular Characteristics of Kidney Renal Papillary Cell Carcinoma
Authors: Gong Yubo , Zhang Chenchen , Li Hao , Yu Xiaojie , Li Yuejia , Liu Zhiguo , He Ruyi
Published: February 2024

Kidney renal papillary cell carcinoma (KIRP) is a highly heterogeneous type of kidney cancer, resulting in limited effective prognostic targets for KIRP patients. Long non-coding RNAs (lncRNAs) have emerged as crucial regulators in the regulation of ferroptosis and iron metabolism, making them potential targets for the treatment and prognosis of KIRP. In this study, we constructed a ferroptosis-related lncRNA risk score model (FRM) based on the TCGA-KIRP dataset, which represents a novel subtype of KIRP not previously reported. The model demonstrated promising diagnostic accuracy and holds potential for clinical translation. We observed significant differences in metabolic activities, immune microenvironment, mutation landscape, ferroptosis sensitivity, and drug sensitivity between different risk groups. The high-risk groups exhibit significantly higher fractions of cancer-associated fibroblasts (CAFs), hematopoietic stem cells (HSC), and pericytes. Drugs (IC50) analysis provided a range of medication options based on different FRM typing. Additionally, we employed single-cell transcriptomics to further analyze the impact of immune invasion on the occurrence and development of KIRP. Overall, we have developed an accurate prognostic model based on the expression patterns of ferroptosis-related lncRNAs for KIRP. This model has the potential to contribute to the evaluation of patient prognosis, molecular characteristics, and treatment modalities, and can be further translated into clinical applications.
The Role of Bioactives in Inflammation
Authors: Hsu Po-Chih , Liu Guan-Ting , Wu Jiunn-Sheng , Kuo Chan-Yen
Published: February 2024

As a physiological defense mechanism, inflammation is a complex response to harmful stimuli [...]
Delamination Techniques of Waste Solar Panels: A Review
Authors: Ghahremani Ali , Adams Scott D. , Norton Michael , Khoo Sui Yang , Kouzani Abbas Z.
Published: February 2024

Solar panels are an environmentally friendly alternative to fossil fuels; however, their useful life is limited to approximately 25 years, after which they become a waste management issue. Proper management and recycling of end-of-life (EOL) solar panels are paramount. It protects the environment because of the high energy consumption of silicon production. We can effectively decrease energy and cost requirements by recovering silicon from recycled solar panels. This is one-third of those needed for manufacturing silicon directly. Moreover, solar panels include heavy metals, such as lead, tin, and cadmium, which pose risks to human health and the environment. Empirical evidence suggests that the costs of mining materials can exceed those of recycled materials, thereby making recycling a more cost-effective means of resource harvesting. This review paper focuses on the techniques developed to delaminate solar panels, which are considered a crucial step in the recycling of EOL solar panels. Initially, various classifications of solar panels are given. Subsequently, an analysis of the diverse methods of solar panel delamination and their efficacy in the retrieval of valued materials is presented. This investigation has identified three primary modes of delamination, namely mechanical, thermal, and chemical. Among these, mechanical delamination is deemed to be a sustainable and cost-effective option when compared to thermal and chemical delamination. The current most popular method of thermal delamination is characterized by its high energy consumption and potential emission, and the chemical delamination generates hazardous liquids that pose their own threat to the environment. This study emphasizes the mechanical delamination techniques, characterized by their environmentally friendly nature, minimal ecological footprint, and capacity to retrieve entire glass panels intact. This paper also discusses the current gaps and potential enhancements for mechanical delamination techniques. For example, some delamination techniques result in crushed materials. Thus, the handling and recovery of materials such as glass and silicon cells require the implementation of an appropriate sorting technique. Also, the value obtained from recovering crushed materials is lower than that of intact glass and silicon cells.
The Relationship between Vitamin D Levels and Blood Glucose and Cholesterol Levels
Authors: Elsheikh Eman , Alabdullah Abdulhakim Ibrahim , Al-Harbi Sarah Saleh , Alagha Amal Omar , AlAhmed Dhiyaa Hassan , Alalmaee Mazen Moraya Ali
Published: February 2024

Background: Vitamin D deficiency has reached epidemic proportions globally. Observational data link low vitamin D status to diabetes, dyslipidemia, and metabolic syndrome, but interventional trials on the effects of supplementation are limited. Objective: We investigated associations between serum 25-hydroxyvitamin D (25(OH)D) levels and metabolic markers in Saudi adults. Methods: This retrospective cross-sectional study analyzed the clinical records of 476 patients from Saudi Arabia, aged 15–78 years. According to 25(OH)D levels, participants were stratified as vitamin D-sufficient (≥30 ng/mL), -insufficient (21–29 ng/mL), or -deficient (≤20 ng/mL). The outcomes were diabetic status (fasting glucose, HbA1c) and lipid panel results. Results: Higher diabetes prevalence was significantly associated with lower 25(OH)D levels (10.1% in the sufficient group, 11.6% in the insufficient group, and 18.3% in the deficient group). Similarly, worse lipid profiles were associated with more severe hypovitaminosis D, including a total cholesterol level of ≥240 mg/dL (5.3% in participants with normal vitamin D levels vs. 18.9% in those with deficient levels) and LDL ≥ 160 mg/dL (6.9% in participants with normal vitamin D levels vs. 13.2% in those with deficient levels). Vitamin D deficiency disproportionately affected women and adults > 45 years old. Conclusions: Vitamin D deficiency is endemic in Saudi Arabia and strongly linked to worsened metabolic markers. Optimizing vitamin D status through screening and correcting the deficiency may provide a cost-effective approach to confronting the regional diabetes epidemic and reducing cardiovascular disease risk.
Performance of Phenolic-Epoxy Coatings after Exposure to High Temperatures
Authors: Ahmed Saleh , Lepkova Katerina , Sun Xiao , Rickard William D. A. , Pojtanabuntoeng Thunyaluk
Published: February 2024

Phenolic-epoxy coatings, which are designed to protect substrates from thermal damage, are widely applied in many fields. There remains an inadequate understanding of how such coatings change during their service life after exposure to various temperature conditions. To further elucidate this issue, this case study investigated the effects of high temperatures on carbon steel panels coated with phenolic epoxy and exposed to different heating conditions. A general trend of decreasing barrier performance was observed after exposure to 150 °C for 3 d, as evidenced by the appearance of cracks on the panel surfaces. In contrast, the coating performance improved after exposure to isothermal conditions (120 °C) or thermal cycling from room temperature to 120 °C, as indicated by the increased low-frequency impedance modulus values of the coating. This unexpected improvement was further examined by characterising the coatings using transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), pull-off adhesion tests, and time-of-flight secondary ion mass spectrometry (ToF-SIMS). The maximum pull-off adhesion force (24.9 ± 3.6 MPa) was measured after thermal cycling for 40 d.
Effect of Spark Plasma Sintering Temperature on the Microstructure and Thermophysical Properties of High-Silicon–Aluminum Composites
Authors: Kong Zhaoyang , Wang Zhipeng , Li Yingmin , Li Runxia
Published: February 2024

Spark plasma sintering is a process of rapid, low-temperature, and high-density sintering. Moreover, traditional sintering methods can solve the problems of large grain sizes and low densities. The sintering temperature plays a crucial role in influencing the physical properties of high-silicon–aluminum (Si-Al) composites. This work investigated the impact of temperature on the microstructure, interface, and physical properties of high-Si-Al composites by spark plasma sintering. The results demonstrate that when the powder was processed by ball milling at a sintering temperature of 565 °C, the material exhibited the densest microstructure with minimal pore formation. The average size of the silicon phase is the smallest. The material’s thermal conductivity is 134.6 W/m·K, the thermal expansion coefficient is 8.55 × 10−6 K−1, the Brinell hardness is 219 HBW, the density is 2.415 g/cm3, and the density reaches 97.75%. An appropriate sintering temperature facilitates particle rearrangement and dissolution–precipitation processes, enhancing the material structure and performance.
Brazing Temperature Effects on the Microstructure and Mechanical Properties of Ti-45Al-8Nb Joints Using TiZrCuNi Amorphous Interlayer
Authors: Wang Sheng , Xu Tianle , Wu Yingchen , Chen Xiguo , Yang Xiaohong
Published: February 2024

Ti-45Al-8Nb alloy is widely utilized in the lightweight design of the aerospace field because of its excellent properties. In order to make full use of this alloy, it is important to carry out relevant research, such as into the joining process of Ti-45Al-8Nb alloy. In this work, Ti-45Al-8Nb alloys were successfully connected by a TiZrCuNi amorphous interlayer, which was fabricated using the rapid solidification method. Ti-45Al-8Nb joints were composed of two zones. The typical microstructure of a Ti-45Al-8Nb joint was Ti-45Al-8Nb/AlCuTi + Ti3Al/(Ti, Zr)(Cu, Ni) + (Ti, Zr)2(Cu, Ni)/Ti3Al + AlCuTi/Ti-45Al-8Nb. The diffusion of elements between the interlayer and the substrate was enhanced by increasing the brazing temperature, which resulted in an increase in the thickness of the interfacial reaction layer. The maximum shear strength was 171.2 MPa, which was obtained at 930 °C. The typical cleavage fracture was found in all of the Ti-45Al-8Nb joints. The mechanical properties of the joint were compromised at high brazing temperature due to the presence of excessive (Ti, Zr)2(Cu, Ni) phase and coarse Ti3Al phase, both of which are inherently brittle and harmful to the shear strength of the obtained joint.
Contribution of Magnetization Mechanisms in MnZn Ferrites with Different Grain Sizes and Sintering Densification
Authors: Liu Hai , Liao Jihong , Li Chonghua , Huang Gang
Published: February 2024

This study investigates the magnetization mechanisms in MnZn ferrites, which are key materials in high-frequency power electronics, to understand their behavior under various sintering conditions. Employing X-ray diffraction and scanning electron microscopy, we analyzed the microstructure and phase purity of ferrites sintered at different temperatures. Our findings confirm consistent spinel structures and highlight significant grain-growth and densification variabilities. Magnetic properties, particularly the saturation magnetization (Ms) and initial permeability (μi), were explored, revealing their direct correlation with the sintering process. The decomposition of magnetic spectra into domain-wall-motion and spin-rotation components offered insights into the dominant magnetization mechanisms, with the domain wall movement becoming increasingly significant at higher sintering temperatures. The samples sintered at 1310 °C showcased superior permeability and the least loss in our investigations. This research underscores the impact of sintering conditions on the magnetic behavior of MnZn ferrites, providing valuable guidelines for optimizing their magnetic performance in advanced electronic applications and contributing to the material science field’s understanding of the interplay between sintering, microstructures, and magnetic properties.
Kidney Stones: Crystal Characterization
Authors: González-Enguita Carmen , García-Giménez Rosario
Published: February 2024

The variety of types that appear in kidney stones makes it necessary to describe them to try to understand their formation. The crystalline phases recognized in stones are classified according to their chemical composition. For this reason, a study using X-ray microdiffraction, Raman spectroscopy, SEM/EDX analysis, and cathodoluminescence (CL) is recommended, to identify their composition. In the present study, this was carried out on kidney stones collected from surgeries in hospitals from Spain. The samples were collected in two representative urban centers: one from a large city, Madrid; the other with a mostly rural population, in order to contrast the possible compositions of the kidney stones. In large cities, struvite appears more frequently, although calcium oxalates are very common in both areas. An attempt was made to provide a guide to the most frequent phases, accompanied by four analysis methods that make their identification possible.
Investigation into the Effects of Cross-Sectional Shape and Size on the Light-Extraction Efficiency of GaN-Based Blue Nanorod Light-Emitting Diode Structures
Authors: Lee Bohae , Ryu Han-Youl
Published: February 2024

We investigated the effect of cross-sectional shape and size on the light-extraction efficiency (LEE) of GaN-based blue nanorod light-emitting diode (LED) structures using numerical simulations based on finite-difference time-domain methods. For accurate determination, the LEE and far-field pattern (FFP) were evaluated by averaging them over emission spectra, polarization, and source positions inside the nanorod. The LEE decreased as rod size increased, owing to the nanorods’ increased ratio of cross-sectional area to sidewall area. We compared circular, square, triangular, and hexagonal cross-sectional shapes in this study. To date, nanorod LEDs with circular cross sections have been mainly demonstrated experimentally. However, circular shapes were found to show the lowest LEE, which is attributed to the coupling with whispering-gallery modes. For the total emission of the nanorod, the triangular cross section exhibited the highest LEE. When the angular dependence of the LEE was calculated using the FFP simulation results, the triangular and hexagonal shapes showed relatively high LEEs for direction emission. The simulation results presented in this study are expected to be useful in designing high-efficiency nanorod LED structures with optimum nanorod shape and dimensions.
Improving the Energy Storage Performance of Barium Titanate-Based Ceramics through the Addition of ZnO-Bi2O3-SiO2 Glass
Authors: Xiong Peifeng , Xiao Man , Yao Zhonghua , Liu Hanxing , Hao Hua
Published: February 2024

Lead-free ceramics with excellent energy storage performance are important for high-power energy storage devices. In this study, 0.9BaTiO3-0.1Bi(Mg2/3Nb1/3)O3 (BT-BMN) ceramics with x wt% ZnO-Bi2O3-SiO2 (ZBS) (x = 2, 4, 6, 8, 10) glass additives were fabricated using the solid-state reaction method. X-ray diffraction (XRD) analysis revealed that the ZBS glass-added ceramics exhibited a perovskite structure, with the maximum relative density achieved at x = 6. The average grain size reduced obviously as the glass additive wt% increased. Also, the dielectric constant decreased and the breakdown strength increased with increases in the glass additives. The optimal energy storage density of 1.39 J/cm3 with an energy storage efficiency of 78.3% was obtained at x = 6 due to high maximum polarization and enhanced breakdown strength. The results demonstrate that this material is a potential candidate for high-pulse-power energy storage devices.