bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effect of Stress on the Skin Welfare in Lumpfish (Cyclopterus lumpus) Broodstock
Authors: Jonassen Thor Magne , Imsland Albert Kjartan Dagbjartarson , Pittman Karin Anna
Published: September 2024

The aim of the experiment was to investigate how induced stress can affect skin welfare of broodstock of lumpfish. From a population of approx. 300 fish of 1587 g (SEM ± 704 g), a sub-population of 20 fish were injected with 30 mg/kg fish cortisol implants 5 January 2018. The control group was not treated with implants. The stress-induced cortisol implant group showed elevated plasma cortisol over a period of approximately one month. Analyses of mucus cell area and density suggested that induced stress could produce a positive functional response (stimulus) in lumpfish early in the experiment, through a gentle increase in the barrier strength of the skin, and reduced mucus cell size and increased density of mucous cells, resulting in a strengthening of the respiratory capacity of the gills. Mucous cell density and calculated barrier strength in the skin were significantly negatively correlated with plasma cortisol, while in the gill filaments of females there was a significant negative correlation between mucous cell density and estrogen levels. The reduced volumetric density of "empty" cells (Q cells) after stress inducement indicates that these cells are important for the maintenance of homeostasis. The fish in this experiment were considered more robust compared to previous analyses on smaller lumpfish.
Research on a Key Method for the Optimization of Port Vessel Detection Based on an Improved Multi-Structural Morphology Approach
Authors: Tabi Fouda Bernard Marie , Zhang Wenjun , Atangana Jacques , Edima-Durand Hélène Carole
Published: September 2024

Some vessels do not comply with maritime rules, particularly in port and safety zones; they must be detected to avoid incidents. Due to background noise and congestion interference at the sea surface, it is difficult to accurately detect these vessels when it is getting dark, especially smaller ones. For these reasons, this paper proposes and develops an improved multi-structural mor-phology (IMSM) approach to eliminate all this noise and interference. The vessel target is sepa-rated from the sea surface backdrop using weighted morphological filtering of many groups of structural components. Then, the neighborhood-based adaptive fast median filtering is used to filter out impulse noise. Finally, the characteristic morphological model of the vessel target is established using the connected domain; this permits eliminating the sea surface congestion and locating vessels movement in real-time. Multiple tests were carried out in a small and discon-tinuous area of the vessel’s inter-frame motion. The results of many groups of data collected show that the proposed approach can effectively eliminate the background noise and congestion in-terference in the monitoring video. The detection accuracy rate and the processing time have increased by approximately 3.91% and 1.14s, respectively.
Properties and Photocuring Behavior of Ceramic Polymer Composite Slurries with Varying Concentration of Silane Coupling Agent
Authors: Ryu Kook-Hyun , Kim Ung-Soo , Kim Jin-Ho , Choi Jung-Hoon , Han Kyu-Sung
Published: September 2024

In this study, 3-trimethoxy-silylpropane-1-thiol (MPTMS) was used as a surface modifier for Al2O3 powder to systematically analyze the effects of MPTMS concentration on the rheological properties, photocuring characteristics, and 3D printing performance of photocurable composite slurries. MPTMS concentration significantly influenced the rheological behavior of the slurry. Slurries containing 2 wt.% and 5 wt.% MPTMS exhibited a wide linear viscoelastic range (LVR). However, at concentrations of 10 wt.% and 20 wt.%, the LVR range narrowed, which led to reduced dispersion stability. In dispersion stability tests, the slurry with 2 wt.% MPTMS showed the most stable dispersion, while the 5 wt.% MPTMS concentration exhibited the highest photocuring rate. In 3D printing experiments, the 5 wt.% MPTMS concentration resulted in the most stable printed structures, whereas printing failures occurred with the 2 wt.% concentration. At 10 wt.% and 20 wt.%, internal cracking was observed, leading to structural defects. In conclusion, MPTMS forms silane bonds on the Al2O3 surface, significantly impacting the stability, rheological properties, and printing quality of Al2O3-acrylate composite slurries. An MPTMS concentration of 5 wt.% was found to be optimal, contributing to the formation of stable and robust structures.
Blood-Brain Barrier Integrity Disruption is Associated to both Chronic Vascular Risk Factors and White Matter Hyperintensities
Authors: Chen James Xiao Yuan , Vipin Ashwati , Sandhu Gurveen Kaur , Leow Yi Jin , Zailan Fatin Zahra , Tanoto Pricilia , Lee Ee Soo , Lee Khang Leng , Cheung Christine , Kandiah Negaenderan
Published: September 2024

Cardiovascular risk factors (CRFs) such as hypertension, high cholesterol, and diabetes are increasingly implicated in cognitive decline and cerebral small vessel disease (cSVD). White matter hyperintensities (WMH), a hallmark of cSVD, are strongly linked to cognitive impairment, but their underlying mechanisms remain unclear. Blood-brain barrier (BBB) dysfunction has been proposed as a potential contributor to WMH development. This study aimed to explore the relationships between CRFs, BBB integrity, and WMH burden. We analyzed 155 participants from the Biomarkers and Cognition Study, Singapore, assessing CRFs through blood tests for glucose and lipid profiles, alongside blood pressure measurements. WMH volume was quantified using MRI, and an in vitro assay evaluated the impact of plasma on BBB integrity using human brain microvascular endothelial cells. Results showed that plasma from participants with higher WMH burden correlated with increased BBB disruption, which was further associated with higher blood pressure, elevated blood glucose, and lipid levels. Both periventricular and subcortical WMH were linked to BBB disruption. These findings suggest that CRFs contribute to BBB dysfunction, promoting WMH development and cognitive decline in cSVD. Further research is needed to clarify the precise mechanisms driving these associations.
Genetic Engineering in Bacteria, Fungi, and Oomycetes
Authors: Yang Piao , Condrich Abraham , Lu Ling , Scranton Sean , Hebner Camina , Sheykhhasan Mohsen , Ali Muhammad Azam
Published: September 2024

Genetic engineering has revolutionized our ability to modify microorganisms for various applications in agriculture, medicine, and industry. This review examines recent advances in genetic engineering techniques for bacteria, fungi, and oomycetes, with a focus on CRISPR-Cas systems. In bacteria, CRISPR-Cas9 has enabled precise genome editing, enhancing applications in antibiotic production and metabolic engineering. For fungi, despite challenges associated with their complex cell structures, CRISPR/Cas9 has advanced the production of enzymes and secondary metabolites. In oomycetes, significant plant pathogens, modified Agrobacterium-mediated transformation and CRISPR/Cas12a have contributed to developing disease-resistant crops. The review provides a comparative analysis of genetic engineering efficiencies across these microorganisms and addresses ethical and regulatory considerations. Future research directions include refining genetic tools to improve efficiency and expand applicability in non-model organisms. This comprehensive overview highlights the transformative potential of genetic engineering in microbiology and its implications for addressing global challenges in agriculture, medicine, and biotechnology.
Protonation Is Part of Dielectric Relaxation upon Reduction of Bacteriopheophytin in Reaction Center of Photosynthetic Bacterium Rhodobacter sphaeroides
Authors: Sipka Gábor , Maróti Péter
Published: September 2024

The pH dependence of the free energy level of the flash induced primary charge pair P+IA– was determined by combination of the results from indirect charge recombination of P+QA– and from delayed fluorescence of the excited dimer (P*) in reaction center of photosynthetic bacterium Rhodobacter sphaeroides where the native ubiquinone at the primary quinone binding site QA was replaced by low potential anthraquinone derivatives. The following observations were made: 1) The free energy state of P+IA– was pH independent below pH 10 (–370 ± 10 meV relative to that of the excited dimer P*) and showed remarkable decrease (about 20 meV/pH unit) above pH 10. A not insignificant part of the dielectric relaxation of the P+IA– charge pair (about 120 meV) should come from protonation related changes. 2) The single exponential decay character of the kinetics proves that the protonated/unprotonated P+IA– and P+QA– states are in equilibria and the rate constants of protonation konH +koffH is much larger than that of the charge back reaction kback ~103 s-1. 3) Highly similar pH profiles were measured for the free energy states of P+QA– and P+IA–, indicating that the same acidic cluster around QB should response to both anionic species. This was supported by model calculations based on anticooperative proton distribution in the cluster with key residues of GluL212, AspL213, AspM17 and GluH173 and on effect of polarization of the aqueous phase on the electrostatic interaction. The larger distance of IA– from the cluster (25.2 Å) than that of QA‒ (14.5 Å) is compensated by smaller effective dielectric constant (6.5 ± 0.5 and 10.0 ± 0.5, respectively). 4) The P* → P+QA− and IA‒QA → IAQA− electron transfers are enthalpy driven reactions with exemption of very large (>60%) or negligible entropic contributions in cases of substitution by 2,3-dimethyl-AQ or 1-chloro-AQ, respectively. The possible structural consequences are discussed.
Robin’s Criterion on Divisibility (II)
Authors: Vega Frank
Published: September 2024

Robin's criterion states that the Riemann hypothesis is true if and only if the inequality $\sigma(n) < e^{\gamma } \cdot n \cdot \log \log n$ holds for all natural numbers $n > 5040$, where $\sigma(n)$ is the sum-of-divisors function of $n$ and $\gamma \approx 0.57721$ is the Euler-Mascheroni constant. We show that the Robin inequality is true for all natural numbers $n > 5040$ that are not divisible by some prime between $2$ and $1771559$. We prove that the Robin inequality holds when $\frac{\pi^{2}}{6} \cdot \log\log n' \leq \log\log n$ for some $n>5040$ where $n'$ is the square free kernel of the natural number $n$. The possible smallest counterexample $n > 5040$ of the Robin inequality implies that $q_{m} > e^{31.018189471}$, $1 < \frac{(1 + \frac{1.2762}{\log q_{m}}) \cdot \log(1.006479799241)}{\log \log n}+ \frac{\log N_{m}}{\log n}$, $(\log n)^{\beta_{n}} < 1.000208229291\cdot\log(N_{m})$ and $n < (1.006479799241)^{m} \cdot N_{m}$, where $N_{m} = \prod_{i = 1}^{m} q_{i}$ is the primorial number of order $m$, $q_{m}$ is the largest prime divisor of $n$ and $\beta_{n} = \prod_{i = 1}^{m} \frac{q_{i}^{a_{i}+1}}{q_{i}^{a_{i}+1}-1}$ when $n$ is an Hardy-Ramanujan integer of the form $\prod_{i=1}^{m} q_{i}^{a_{i}}$. By combining these results, we present a proof of the Riemann hypothesis. This work is an expansion and refinement of the article "Robin's criterion on divisibility", published in The Ramanujan Journal.
Fungal Elicitor-Induced Resistance Against Pine Wilt Disease in Pinus densiflora
Authors: Moon Young Bum , Kim Yi Rae , Han Jung Yeon , Choi Yong Eui
Published: September 2024

Pinosylvin and its methylated derivatives, pinosylvin monomethyl ether (PME) and dihydropinosylvin monomethyl ether (DPME), are representative phytoalexins found in Pinus species. Both PME and DPME have strong nematicidal activity against pine wood nematodes (PWNs). In this study, we investigated the resistance of Pinus densiflora to PWN after trunk injection or soil drenching with a fungal elicitor prepared from Penicillium chrysogenum. Fungal elicitor treatment by soil drenching resulted in increased accumulation of PME and DPME in the needles of the plants. When the pine trees without fungal elicitor pretreatment were inoculated with PWNs, 67.9% died after five months, but only 33.3% died among the plants pretreated with the fungal elicitor. Injection of the fungal elicitor into the trunks of P. densiflora trees also strongly increased the synthesis of PMEs. The concentration of PME in the leaves of P. densiflora reached a maximum (155.4 µg/g DW) after 4 weeks of fungal elicitor injection. The second injection of the elicitor after the first injection of the fungal elicitor also increased the accumulation of PME. To investigate PWN resistance in P. densiflora after two months of trunk injection of elicitor, the downward movement of PWNs from the top of branches to the bottom was monitored after PWNs were inoculated on the holes of the upper internodal segments of branches. The number and mobility of PWNs that moved from the bottom of branches were highly reduced in fungal elicitor-injected pine plants. This result suggests an eco-friendly approach for eliciting-induced PWN resistance in PWN-susceptible P. densiflora via fungal elicitor treatment.
Comparative Evaluation of Post-Thaw Viability and In Vitro Fertility Between Ejaculated and Epididymal Semen, and Characterization of Seminal cfDNA in Pantaneiro Bulls
Authors: Carneiro Marcos Coura , Castro Alice Caroline Souza , Silva Roberta Reis , Chaves José Eduardo Vieira , Silva Venâncio Augusto Oliveira , Capobianco Natalia Ernandes , Queiroz Paulo José Bastos , Melo Leonardo de França e , Barbosa Eleonora Araújo , Dode Margot Alves Nunes , Spricigo José Felipe Warmling
Published: September 2024

This study evaluated the post-thaw viability and in vitro fertility of ejaculated and epididymal semen from Pantaneiro bulls and characterized cell-free DNA (cfDNA) in fresh seminal plasma. Semen from five bulls was collected via electroejaculation or post-mortem epididymal extraction. Fresh semen parameters and cfDNA concentrations were assessed before cryopreservation. Post-thaw sperm kinetics were evaluated using CASA at 0 and 6 hours of incubation, and in vitro embryo development was analyzed following IVF. Data were assessed using ANOVA and logistic regression. Ejaculate samples exhibited more morphological defects than epididymal samples (15.8% vs. 1.8%, P≤0.05). Post-thaw, epididymal semen showed higher total (87.2% vs. 32.4%) and progressive (67.1% vs. 14.4%) motility at 0 h (P≤0.05), and higher motility at 6 h (38.9% vs. 11.0%, P≤0.05). In vitro fertility did not differ significantly between ejaculated (n=525 oocytes) and epididymal (n=500 oocytes) semen groups in terms of cleavage (49.6% vs. 44.2%) and blastocyst formation on D7 (26.1% vs. 22.2%, P>0.05). cfDNA concentration in fresh semen ranged from 11.4 to 50.9 ng/µL. These findings indicate that epididymal sperm from Pantaneiro bulls retain high post-thaw viability and fertility. Additionally, cfDNA characterization in seminal plasma contributes to indigenous cattle preservation and advances in male fertility research.
The Microbiota of the Outer Gut Mucus Layer of the Migrating Northeast Arctic Cod (Gadus morhua) as Determined by Shotgun DNA Sequencing
Authors: Le Doujet Typhaine , Haugen Peik
Published: September 2024

Animals form functional units with their microbial communities, termed metaorganisms. While extensive research has been conducted on a few model animals, much remains unknown about the microbial diversity in most species. Here, we describe the taxonomic profile of the microbes from the outer gut mucus layer from the Northeast Arctic cod or also called the migrating Atlantic cod using a shotgun DNA sequencing approach. We provide metagenomes from six individuals. The results reveal three different taxonomic profile “types": Type I is dominated in numbers by Pseudomonaceae (44%) and Xanthomonadaceae (13%), Type II contains 65% Vibrionaceae and Type III contains 76% Enterobacteriaceae. This stands in sharp contrast to the bacterial diversity of the transient gut content (i.e., feces). Additionally, binning of assembled reads followed by phylogenomic analyses place a high-completeness bin of Type I within the Pseudomonas fluorescens group, one bin of Type II within the Photobacterium phosphoreum clade, and finally a bin of Type III within the Escherichia/Shigella group. In conclusion, we describe the adherent bacterial diversity in the Northeast Arctic cod's intestine using shotgun sequencing, revealing different taxonomic profiles compared to the more homogenous transient (fecal) microbiota. This suggests that the intestine contains two separate and distinct microbial populations.
Evaluation of an Alternative Screening Method for Gestational Diabetes Diagnosis During the COVID-19 Pandemic (DIABECOVID STUDY): An Observational Cohort Study
Authors: Casellas Alba , Martinez Cristina , Amigó Judit , Ferrer Roser , Marti Laia , Merced Carme , Medina M Carmen , Molinero Istria , Calveiro Marta , Maroto Anna , del Barco Ester , Carreras Elena , Goya Maria M.
Published: September 2024

Aim: To evaluate the impact of applying alternative diagnostic criteria for gestational diabetes mellitus (GDM) during the COVID-19 pandemic on GDM prevalence, obstetrical and perinatal outcomes, and costs, as compared to the standard diagnostic method. Methods: A cohort of pregnant individuals undergoing GMD screening with the alternative GDM method, which uses plasma glucose (fasting or non-fasting) and HbA1c, was compared with a cohort of pregnant individuals undergoing the standard GDM screening method. Both cohorts were obtained from 6 hospitals across Catalonia, Spain, from April 2020 to April 2022. The primary outcome was large for gestational age rate at birth. Secondary outcomes were composite adverse outcomes including pregnancy complications, delivery complications, and neonatal complications. Cost differences between screening methods were also evaluated. A similar analysis was performed in the subgroup diagnosed with GDM. Results: Data were collected from 1,543 pregnant individuals in the standard screening group and 2,197 in the alternative screening group. The standard screening group had a higher GDM diagnostic rate than the alternative screening group (10.8% vs 6.9%, respectively; p<0.0001). The primary outcome (large for gestational age rate) was similar between groups: 200/1543 (13.0%) vs 303/2197 (13.8%). The adjusted OR for this outcome was 1.74 (95% CI: 0.74-4.10). An adjusted analysis showed no differences between groups in composite adverse outcomes for pregnancy complications (OR: 1.11; 95% CI: 0.91-1.36), delivery complications (OR: 0.95; 95% CI: 0.75-1.19), and neonatal complications (OR: 1.28; 95% CI: 0.94-1.75). Among individuals diagnosed with GDM, the large for gestational age rate was similar between groups: 13/166 (7.8 %) vs 15/151 (9.9 %). The OR adjusted for this outcome was 1.24 (95% CI: 0.51-3.09). An adjusted analysis showed no differences in composite adverse outcomes for pregnancy complications (OR: 1.57; 95% CI: 0.84-2.98), delivery complications (OR: 1.21; 95% CI: 0.63-2.35), and neonatal complications (OR: 1.35; 95% CI: 0.61-3.04). The mean cost (which included expenses for consumables, equipment and personnel) of the alternative screening method was 46.0 euros (22.3 SD), as compared to 85.6 euros (67.5 SD) for the standard screening method. Conclusions: In this Spanish population during the COVID-19 pandemic, GDM prevalence was lower in the alternative screening group than in the standard screening group. After adjusting for GDM risk factors, outcomes related to obstetrics, delivery, and neonatal complications were comparable between both groups. Finally, the alternative screening method was cheaper than the standard screening method.
The Relationship between Understanding Diversity and Brain Health, Focusing on the Triple Network
Authors: Otsuka Taiko , Kokubun Keisuke , Okamoto Maya , Yamakawa Yoshinori
Published: September 2024

Background/Objectives: Interest in diversity is growing worldwide. Today, understanding and social acceptance of diverse people is becoming increasingly important. Therefore, in this study, we aimed to clarify the relationship between gray matter volume (GMV), which is thought to reflect brain health, and understanding of diversity (gender, LGBTQ, and race). Methods: GMV was the value of the Gray Matter Brain Healthcare Quotient (GM-BHQ) based on MRI image analysis. Meanwhile, diversity was calculated from the answers to the psychological questions included in the World Values Survey Wave 7 (WVS7). Results: As a result of the analysis, in the group of participants with the highest understanding of diversity (PHUD. n = 11), not only the GMV at the whole brain level but also the GMV of the central executive network (CEN) and saliency network (SN) were shown to be significantly higher than the theoretical value estimated from sex, age, and BMI at the 5% level. In addition, the GMV of the default mode network (DMN) was also shown to be higher than the theoretical value at the 10% level. Meanwhile, in the group of others (n = 10), there was no significant difference from the theoretical value. These differences between PHUD and others were also observed when comparing the two. Conclusions: These results suggest that understanding diversity requires a healthy brain, centered on three networks that govern rational judgment, emotion regulation, other-awareness, self-awareness, and the valuing of actions. This is the first study to show that brain structure is related to understanding and acceptance of diverse people.
Development of an Eco-Friendly Nanogel Incorporating Pectis brevipedunculata Essential Oil as Larvicidal Agent Against Aedes aegypti
Authors: Marques Estela Mesquita , Rocha Raiene Lisboa , Brandão Clenilma Marques , Xavier Júlia Karla de Albuquerque Melo , Camara Marcos Bispo Pinheiro , Mendonça Caritas de Jesus Silva , de Lima Roberto Batista , Souza Melissa Pires , Costa Emmanoel Vilaça , Gonçalves Renato Sonchini
Published: September 2024

Arboviruses, transmitted by mosquitoes like Aedes aegypti, pose significant public health challenges globally, particularly in tropical regions. The rapid spread and adaptation of viruses such as Dengue, Zika, and Chikungunya have emphasized the need for innovative control methods. Larvicide property of essential oils has emerged as a potential alternative to conventional insecticides. This work showed the development of an eco-friendly nanogel using a low-energy, solvent-free method, incorporating the copolymer F127 and carbopol 974p with a high concentration of essential oil from Pectis brevipedunculata (Gardner) Sch.Bip. (Pb). The resulting nanogel displayed excellent physical stability and was maintained under varying temperature conditions. Characterization techniques, including FTIR and DLS, confirmed the stable incorporation of essential oil (EOPb) within the nanogel matrix. The in vitro assays against Aedes aegypti larvae revealed that at 500 μg/mL, the mortality rates was 96.0% ± 7.0 after 24 h and 100.0% ± 0.0 after 48 h. The positive control group treated with temephos, achieved 100% mortality at both time points, validating the experimental conditions and providing a benchmark for assessing the efficacy of the nGF2002Pb nanogel. These results indicate that the nGF2002Pb demonstrates a pronounced concentration-dependent larvicidal effect against Aedes aegypti, offering an innovative and sustainable approach for arbovirus vector control.
Radiomics Features of FDG PET Images Predict ApoE4 and may Provide Clues for Further Studies on Alzheimer’s Disease
Authors: Rasi Ramin , Guvenis Albert
Published: September 2024

Objective: Fludeoxyglucose F18 positron emission tomography (FDG PET) can give early clues for diagnosing Alzheimer’s disease (AD). Our objective was to use the same scan to predict Apolipoprotein E4 (ApoE4), known to be a risk factor. A second objective was to determine the brain regions and imaging features associated with this gene allele that can potentially help elucidate the mechanisms of the disease and better comprehend the images. Method: We employed data from 112 participants from the Alzheimer’s Disease Neuroimaging Initiative (ADNI). PET and structural MRI data were co-registered with ANTx, and the Freesurfer tool delineated 95 whole brain, 19 hippocampal, and 9 amygdala regions. Using PyRadiomics we extracted 120 radiomic features from these segments. We employed a hybrid feature selection strategy. The HistGradientBoosting (HGB) classifier was evaluated using stratified five-fold cross-validation. Results: The proposed radiomics model predicted the homozygous ApoE4 genotype, with an AUC of 0.945 an accuracy of 0.875, a sensitivity of 0.914, and a specificity of 0.838. Reducing the feature set to seven key features resulted in a slightly decreased performance (AUC: 0.889). The mean values of the features showed statistically significant (p<0.05) incremental deterioration between different groups of homozygous ApoE4 carriers. Conclusion: Our findings underscore the critical role of specific brain regions and associated features in differentiating ApoE4 carriers. The hippocampus, entorhinal cortex, amygdala, thalamus, and pars orbitalis exhibited significant associations with the ApoE4 genotype. By employing SHAP analysis, we identified key features driving model predictions, enhancing interpretability, and providing insights into the underlying pathophysiology.
Research Progress on CoCrCuNi High-Entropy Alloys
Authors: Zhao Fachang , Zhao Xingming , Jiang Tianzhi , Li Honglin , Liu Xue , Ai Ziqing , Zhao Rongda , Wu Fufa
Published: September 2024

High-entropy alloys have attracted widespread attention from researchers worldwide due to their unique microstructure and outstanding mechanical properties, making them a prominent focus in the field of metallurgy. Among the various high-entropy alloys, the CoCrCuNi system was among the first alloys discovered, and it has shown significant progress in development. By employing different preparation and heat treatment processes, researchers have obtained alloys with diverse performances. The addition of various alloying elements or other components can lead to pronounced variations in the properties of CoCrCuNi high-entropy alloys. This work provides a comprehensive review of recent research progress on CoCrCuNi high-entropy alloys both domestically and internationally. It covers the preparation methods, thermodynamic and kinetic simulation calculations, as well as discussions on heat treatment processes and the influence of alloying elements on the microstructure and mechanical properties of CoCrCuNi high-entropy alloys. Finally, the review concludes with a prospective analysis and predictions for the potential applications and future directions in developing novel high-entropy alloys.
A Realistic Model Reference Computed Torque Control Strategy for Human Lower Limb Exoskeletons
Authors: Hasan Sk K
Published: September 2024

Exoskeleton robots have become a promising tool in neurorehabilitation, offering effective physical therapy and recovery monitoring. The success of these therapies relies on precise motion control systems. Although computed torque control based on inverse dynamics provides a robust theoretical foundation, its practical application in rehabilitation is limited by its sensitivity to model accuracy, making it less effective when dealing with unpredictable payloads. To overcome these limitations, this study introduces a novel model reference computed torque controller that accounts for parametric uncertainties while optimizing computational efficiency. A dynamic model of a seven-degree-of-freedom human lower limb exoskeleton is developed, incorporating a realistic joint friction model to accurately reflect the physical behavior of the robot. To reduce computational demands, the control system is split into two loops: a slower loop that predicts joint torque requirements based on input trajectories and robot dynamics, and a faster PID loop that corrects trajectory tracking errors. Coriolis and centrifugal forces are excluded from the model due to their minimal impact on system dynamics relative to their computational cost. Experimental results show high accuracy in trajectory tracking, and statistical analyses confirm the controller’s robustness and effectiveness in handling parametric uncertainties. This approach presents a promising advancement for improving the stability and performance of exoskeleton-based neurorehabilitation.
Genetic Diversity of Listeria monocytogenes in Fruits and Vegetables: Environmental Persistence and Antimicrobial Resistance
Authors: Avila-Novoa María Guadalupe , Solis-Velazquez Oscar Alberto , Guerrero-Medina Pedro Javier , Martínez-Chávez Liliana , Matínez-Gonzáles Nanci Edid , Gutiérrez-Lomelí Melesio
Published: September 2024

Background/Objectives: Listeria monocytogenes is a foodborne pathogen that can infect humans and animals, causing non-invasive gastrointestinal listeriosis or invasive listeriosis. The objectives of this study were to determine: the genetic diversity of L. monocytogenes; the genes associated with the resistance to antibiotics, benzalkonium chloride (BC), and CdCl2, and biofilm formation. Methods: Seventeen strains of L. monocytogenes isolated fresh fruits and vegetables were selected for this study. Genetic diversity and the genes associated with antibiotic resistance were determined by PCR amplification. Susceptibility to antibiotics were determined using the agar diffusion method. Resistance to BC and CdCl2 was determined using the minimum inhibitory concentration method. Capacity for biofilm formation was evaluated using the crystal violet staining method. Results: This study demonstrates that the isolates of L. monocytogenes belong to serotypes 1/2a (29.4%) and 1/2b (70.5%). Potential hypervirulent strains containing 70.5% Listeria pathogenicity island 1 (LIPI-1-2), 17.6% (LIPI-1-3), and 11.7% (LIPI-1-4) were also identified. Antibiotic susceptibility tests show that L. monocytogenes exhibit six different multiresistant patterns with a MAR index of ³ 0.46 (70.5%); besides, the gen Ide, tetM, and msrA were detected in 52.9%, 29.4%, and 17.6%, respectively, associated with efflux pump Lde, tetracycline and ciprofloxacin resistance. Phenotypic test shows that 58.8% cadmium-resistant L. monocytogenes have a co-resistance to BC of 23.5%. Subsequently, all strains of L. monocytogenes exhibited moderate biofilm. Conclusions: This study contributes to the persistence and genetic diversity of strains L. monocytogenes isolated fresh fruits and vegetables; in addition, resistance to CdCl2 and correlation with co-resistance to BC disinfectant used in the food industry.
Recent Advancements in Applying Machine Learning in Power-to-X Processes: A Literature review
Authors: Shojaei S. Mohammad , Aghamolaei Reihaneh , Ghaani Mohammad Reza
Published: September 2024

For decades, fossil fuels have been the backbone of reliable energy systems, offering unmatched energy density and flexibility. However, as the world shifts toward renewable energy, overcoming the limitations of intermittent power sources requires a bold reimagining of energy storage and integration. Power-to-X (PtX) technologies, which convert excess renewable electricity into storable energy carriers, offer a promising solution for long-term energy storage and sector coupling. Recent advancements in machine learning (ML) have revolutionized PtX systems by enhancing efficiency, scalability, and sustainability. This review provides a detailed analysis of how ML techniques, such as deep reinforcement learning, data-driven optimization, and predictive diagnostics, are driving innovation in Power-to-Gas, Power-to-Liquid, and Power-to-Heat systems. While ML applications have shown great potential in optimizing operational decisions and managing uncertainties in renewable energy integration, challenges such as data quality, real-time processing, and scalability remain. Addressing these gaps presents important future research opportunities. These advancements are critical to decarbonizing hard-to-electrify sectors such as heavy industry, transportation, and aviation, aligning with global sustainability goals.
Introduction to General Theory of Relativity and Interpretation of Geometrical Quantities
Authors: Kamberaj Hiqmet
Published: September 2024

In this paper, we will describe the general theory of relativity. The special theory of relativity is based on the ideal inertial frames. These ideal inertial frames are assumed to exist in every event in space-time. In contrast, in the general theory of relativity, we do not have to make that assumption. Additionally, the space-time is considered to be part of the solution to the motion. Moreover, interpretation of geometrical quantities is introduced.
Optimisation of Windmill Power Plant Epicyclic Gearbox
Authors: Vrcan Željko , Troha Sanjin , Marković Kristina , Marinković Dragan
Published: September 2024

Humanity is shifting its power generation to renewable energy, notably wind power. As more and more wind power plants are built, it has become important to improve the efficiency of wind power generation and reduce the manufacturing costs of wind power equipment. The mechanical multiplier gearbox is one of the most important parts of the wind power turbine, and it is now being built as a planetary gear train as these gearboxes offer a compact build capable of high power ratings in a relatively small package with coaxial input and output shafts. It is very important to select the right kinematic scheme and the other relevant parameters in the early design stages as the wrong kinematic scheme will result in a suboptimal solution. This article deals with the selection of the optimal gearbox solution for a 700 kW wind turbine application, taking into account both two-carrier and three-carrier gearbox solutions. As the optimal solution may be deducted only by systematic analysis, the proprietary 2-SPEED software has been developed for this purpose. The results of the analysis have been presented in this article, and optimal gear train solutions have been suggested for both two-carrier and three-carrier multiplier configurations.
Bias Analysis and Correction in Weighted-L1 Estimators for the First-Order Bifurcating Autoregressive Model
Authors: Elbayoumi Tamer , Mostafa Sayed
Published: September 2024

This study examines the bias in weighted least absolute deviation (WL1) estimation within the context of stationary first-order bifurcating autoregressive (BAR(1)) models, which are frequently employed to analyze binary tree-like data, including applications in cell lineage studies. Initial findings indicate that WL1 estimators can demonstrate substantial and problematic biases, especially when small to moderate sample sizes. The autoregressive parameter and the correlation between model errors influence the volume and direction of the bias. To address this issue, we propose two bootstrap-based bias-corrected estimators for the WL1 estimator. We conduct extensive simulations to assess the performance of these bias-corrected estimators. Our empirical findings demonstrate that these estimators effectively reduce the bias inherent in WL1 estimators, with their performance being particularly pronounced at the extremes of the autoregressive parameter range.
Effects of Hydrogen Dissociation during Gas Flooding on Formation of Metal Hydride Cluster Secondary Ions in SIMS
Authors: Ekar Jernej , Markelj Sabina , Mozetič Miran , Zaplotnik Rok , Kovač Janez
Published: September 2024

The application of hydrogen flooding was recently shown to be a simple and effective approach towards improved layer differentiation and interface determination during SIMS depth profiling of thin films as well as an approach with a potential in the field of quantitative SIMS analyses. To further study the effects of hydrogen, the flooding of H2 molecules was compared to the reactions of the atomic H on the samples of pure metals and their alloys. H2 was introduced into the analytical chamber via a capillary which was, to achieve the dissociation, heated to approximately 2200 K. Dissociation of H2 up to 30% resulted in a significant increase in the intensity of the metal hydride cluster secondary ions originating from the metallic samples. Comparison of time scales of possible processes provided an insight into the mechanism of the hydride cluster secondary ion formation. Cluster ions presumably form during the recombination of the atoms and molecules from the sample and adsorbed atoms and molecules from the gas. This process occurs on the surface or just above it during the sputtering process. These findings coincide with the previous mechanistic and computational studies.
Canopy Type and Its Effects on Photosynthesis and Fruit Quality of Cabernet Sauvignon Grapevines in Northern Mexico
Authors: Hernández-Ordoñez Elizabeth , Cruz-Alvarez Oscar , Orozco-Avitia Jesús Antonio , Hernández-Rodríguez Ofelia Adriana , Alonso-Villegas Rodrigo , Jacobo-Cuellar Juan Luis , Gardea-Bejar Alfonso Antero , Ojeda-Barrios Damaris Leopoldina
Published: September 2024

Modification of canopy architecture is fundamental to productivity in grapevine. This research focused on evaluating the impact of canopy openness on photosynthetically active radiation, photosynthetic activity, and the physicochemical properties of fruit in Cabernet Sauvignon grapevines. A completely randomised design was used to compare open and closed canopies, with ten replicates per treatment (20 trees in total), during the vegetative growth period and after harvest. Key measurements included photosynthetically active radiation (PAR), daily light inte-gral (DLI), photosynthetic rate (PR), stomatal conductance (SC), intercellular CO2 concentration (IC), leaf area (LA), transpiration, ambient CO2 concentration, and temperature. Additionally, we assessed fruit quality parameters such as total soluble solids (TSS), glucose-fructose, total titratable acidity (TTA), pH, TSS/TTA, and total phenols (TP). significantly (p≤0.05) increased PAR, DLI, PR, IC, and LA, while after harvest, only PR and IC showed variation. Closed canopies increased CO2/H2O by 62.5%, while H2O/CO2 and temperature were higher in open canopies. Canopy openness increased contacts, gaps and visible sky and reduced leaf area index. Open canopy fruits showed higher TSS, glucose-fructose, pH, TSS/TTA and TP contents. Canopy openness is essential for improving light interception, photosynthetic efficiency and fruit quality in Cabernet Sauvi-gnon grapevine cultivated in northern Mexico.
Progress in Precision Medicine for Head and Neck Cancer
Authors: Vakili Sanaz , Barzegar Behrooz Amir , Wichelo Rachel , Fernades Alexandra , Emwas Abdul-Hamid , Jaremko Mariusz , Markowski Jarosław , Łos Marek J. , Ghavami Saeid , Vitorino Rui
Published: September 2024

This paper presents a comprehensive comparative analysis of head and neck cancer (HNC) biomarkers, a prevalent but molecularly diverse malignancy. We detail the roles of key proteins and genes in tumorigenesis and progression, emphasizing their diagnostic, prognostic, and therapeutic relevance. Our bioinformatic validation reveals crucial genes such as AURKA, HMGA2, MMP1, PLAU, and SERPINE1, along with microRNAs (miRNA) linked to HNC progression. OncomiRs, including hsa-miR-21-5p, hsa-miR-31-5p, hsa-miR-221-3p, hsa-miR-222-3p, hsa-miR-196a-5p, and hsa-miR-200c-3p, drive tumorigenesis, while tumour-suppressive miRNAs like hsa-miR-375 and hsa-miR-145-5p inhibit it. Notably, hsa-miR-155-3p correlates with survival outcomes in addition to genes RAI14, S1PR5, OSBPL10, and METTL6, highlighting its prognostic potential. Future directions focus on leveraging precision medicine, novel therapeutics, and AI integration to advance personalized treatment strategies to optimize patient outcomes in HNC care.
About Stability of One System of Stochastic Difference Equations with Exponential Nonlinearity
Authors: Shaikhet Leonid
Published: September 2024

A system of two nonlinear difference equations under stochastic perturbations is considered. Nonlinearity of the exponential type in each equation of the system under consideration depends on all variables of the system. The stability in probability of a positive equilibrium of the system is studied via the general method of Lyapunov functionals construction and the method of linear matrix inequalities (LMIs). The obtained results are illustrated via examples and figures with numerical simulations of solutions of a considered system of stochastic difference equations. The proposed way of investigation can be applied to nonlinear systems of higher dimension and with other types of nonlinearity, for both difference equations and for delay differential equations.
Two-Dimensional Directions Determination for GNSS Spoofing Source Based on MEMS-Based Dual-GNSS/INS Integration
Authors: Zhang Chengzhong , Wang Dingjie , Wu Jie
Published: September 2024

Satellite navigation spoofing is a major challenge in the field of satellite/inertial integrated navigation security. To effectively enhance the anti-spoofing capability of low-cost GNSS/MEMS-SINS integrated navigation system, this paper proposes a method integrating a dual-antenna global navigation satellite system (GNSS) and a micro-inertial measurement unit (MIMU) to determine the two-dimensional (2D) directions of spoofing signal sources. The proposed method evaluates whether the single-difference carrier-phase measurements conform to the corresponding directions given in ephemeris files, and employs the micro-inertial navigation technology to determine the 2D directions of the signal source. Based on a set of short-baseline dual-station measurements, the accuracy of the proposed method in determining the 2D azimuths of satellites in synchronous orbits is verified, and the deviation from the real value is evaluated. The experimental results show that the proposed method can effectively identify the spoofed satellite signals while providing high-precision direction information at three different distances: 100m, 10km, and 36000km.The two-dimensional angles errors do not exceed 0.2 rad, 0.05 rad, and 0.01 rad, respectively.
Virtual Reality and Artificial Intelligence Paving the Future of Cardiac Interventions and Training, a Review of the Available Reviews
Authors: AbdelMassih Antoine , Nasser Abdullah , AbdelRahman Gawahir , Mkarem Lama , AbuShashieh Mariam , AbuGhosh Rahaf
Published: September 2024

Several medical graduates, avoid surgical specialties especially those with complex and delicate anatomy due to their doubt in their visual spatial abilities. Many patients are deemed inoperable, particularly in the field of pediatric cardiology, due to the complexity of the anatomy. On a similar note, stratification of risk of cardiac interventions, and decision making, is a room of significant person-based and center-based bias. Two inter-related technologies, namely immersive virtual reality (VR) and artificial intelligence have made impossible things possible and can help to standardize decision making strategies. This review aimed at reviewing the main anatomical and lesion-based scopes of these advancements in the field of cardiac care, with a special focus on pediatric cardiology.
Novel Nonlinear Suspension based on the Concept of Origami Metastructures: Theoretical and Experimental Investigations
Authors: Zippo Antonio , Molaie Moslem , Iarriccio Giovanni , Pellicano Francesco
Published: September 2024

This study presents a comprehensive investigation on an innovative mechanical system inspired by recent advancements in metamaterials, more specifically the work is focused on Origami-type structures for their intriguing mechanical properties. Originating from the specific fields such as aerospace for their lightweight and foldable characteristics, Origami mechanical devices exhibit unique non-linear stiffness, in particular, when suitably designed, they show Quasi-Zero Stiffness (QZS) characteristics within a specific working range. The QZS property, aligned with the High Static Low Dynamic (HSLD) stiffness concept, suggests promising applications such as a low-frequency mechanical passive vibration isolator. The study explores the vibration isolation characteristics of Origami-type suspensions, with a particular emphasis on their potential application as low-frequency passive vibration isolators. The Kresling Origami Module (KOM) has been selected for its compactness and compatibility with 3D printers. A detailed analysis using 3D CAD, Finite Element Analysis, and experimental testing have been carried out. The investigation includes the analysis of the influence of geometric parameters on the non-linear Force-Displacement curve. Multibody simulations validate the low-frequency isolation properties within the QZS region, and disparities in dynamic properties beyond the QZS range. The study underscores the transformative potential of Origami-type metamaterials in enhancing low-frequency vibration isolation technology. It also highlights challenges related to material properties and loading mass variations, providing valuable insights for future developments in this promising field.
Unraveling the Omega-3 Puzzle: Navigating Challenges and Innovations for Bone Health and Healthy Aging
Authors: Ali Zayana , Al-Ghouti Mohammad A. , Abou-Saleh Haissam , Rahman Md Mizanur
Published: September 2024

Omega-3 polyunsaturated fatty acids (ω-3 PUFAs, n-3 PUFAs), including eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and alpha-linolenic acid (ALA), are essential polyunsatu-rated fats primarily obtained from fatty fish and plant-based sources. Compelling evidence from preclinical and epidemiological studies consistently suggests beneficial effects of ω-3 PUFAs on bone health and healthy aging processes. However, clinical trials have yielded mixed results, with some failing to replicate these benefits seen in preclinical models. This contraindication is mainly due to challenges such as low bioavailability, potential adverse effects with higher doses, and susceptibility to oxidation of ω-3 fatty acids, hindering their clinical effectiveness. This review provides an in-depth analysis of recent findings, focusing on clinical perspectives and encom-passing all relevant published studies to date, including preclinical and epidemiological research, with an emphasis on the role of omega-3 fatty acids in promoting bone health and supporting healthy aging. Additionally, it explores strategies to improve ω-3 PUFA efficacy, including na-noparticle encapsulation and incorporation of specialized pro-resolving mediators (SPM) derived from DHA and EPA, to mitigate oxidation and enhance solubility, thereby improving therapeutic potential. By consolidating evidence from various studies, this review underscores current in-sights and future directions in leveraging ω-3 PUFAs for therapeutic applications.