bluuebunny/crossref_metadata_2025 huggingface dataset:
OB Stars and Cepheids From the Gaia TGAS Catalogue: Test of their Distances and Proper Motions
Authors: Bobylev Vadim V. , Bajkova Anisa T.
Published: December 2017

We consider young distant stars from the Gaia TGAS catalog. These are 250 classical Cepheids and 244 OB stars located at distances up to 4 kpc from the Sun. These stars are used to determine the Galactic rotation parameters using both trigonometric parallaxes and proper motions of the TGAS stars. In this case the considered stars have relative parallax errors less than 200%. Following the well-known statistical approach, we assume that the kinematic parameters found from the line-of-sight velocities Vr are less dependent on errors of distances than the found from the velocity components Vl. From values of the first derivative of the Galactic rotation angular velocity ′0, found from the analysis of velocities Vr and Vl separately, the scale factor of distances is determined.We found that from the sample of Cepheids the scale of distances of the TGAS should be reduced by 3%, and from the sample of OB stars, on the contrary, the scale should be increased by 9%.
Worlds of Transitive Identities
Authors: Sbiri Kamal
Published: December 2017

In studying certain autobiographical narratives of forced migration and exile, the experience of being caught between two worlds is always emphasized. The forced migrant narrative provides different pictures of the journey from homeland to borderland, and highlights on the ruptures that identity undergoes, especially when the migrant seeks to link the pain of identity construction at a liminal phase. The experience of being lost is understood as a double movement, where in either case, identity loses its originality. Both the forced immigrant and her/his native speaker counterpart seem to exercise similar roles of translation, that is to understand and be understood. In my study of two contemporary autobiographical narratives of forced migrants, I would like to investigate how translation can help in conflating ruptures and identity construction in a transnational context. By leaning on Stephen Clingman’s theory on transnational fiction and Said’s contrapuntal analysis, I seek to emphasize the transitive elements that reside in the immigrant’s psyche. Based on this assumption, I seek to argue that both navigation within the self and recognition of displacement as an experience of mobility encourage the forced immigrant to realize her singularity as transnational.
On TSVD regularization for a Broyden-type algorithm
Authors: Smirnova Alexandra
Published: December 2017

An applied inverse problem in the form of a nonlinear operator equation on a pair of two Hilbert spaces is considered. In the lack of stability, a version of Broyden’s method regularized by truncated singular value decomposition (TSVD)
is proposed in order to suppress the potential noise propagation and to reduce the cost associated with storage and inversion of the Fréchet
derivative operator. An attractive feature of this study is that it does not rely on any restrictions on the nonlinearity of the operator and/or on the spectrum of the Fréchet
derivative. For the numerical analysis of the proposed algorithm, a parameter identification problem in epidemiology is investigated.
Whither the Middle Class? Financialization, Labor Institutions, and the Gap between Top- and Middle-Income Earners in Advanced Industrial Societies
Authors: Kwon Roy , Roberts Anthony , Zingula Karissa
Published: December 2017

Recent research shows that financial activities expand the wage gap between affluent and middle-class workers in advanced industrial societies. Furthermore, a well-established literature indicates that differences in labor institutions may be responsible for cross-national variations of income inequality in developed countries. Surprisingly, there is no empirical research examining whether the positive association between financialization and income inequality is conditioned by differences in wage coordination. We contribute to this comparative income inequality literature by testing the claim that wage-setting institutions suppress the inequality-widening effects of finance in advanced industrial societies. To test this contention, we compile an unbalanced panel dataset of 20 developed economies during the years 1988 to 2009. According to our results, financial activities are a robust positive predictor of 90–50 inequality. More importantly, the interaction of financialization and wage coordination returns significant negative associations with the dependent variable. These results are found to be consistent across different estimation techniques and numerous regression parameters.
Natural and environmental oestrogens induce TGFB1 synthesis in oviduct cells
Authors: Cometti Barbara P S , Dubey Raghvendra K , Imthurn Bruno , Rosselli Marinella
Published: December 2017

Autocrine/paracrine factors generated in response to 17β-oestradiol (E2), within the oviduct, facilitate early embryo development for implantation. Since transforming growth factor beta 1 (TGFB1) plays a key role in embryo implantation, regulation of its synthesis by E2 may be of biological/pathophysiological relevance. Here, we investigated whether oviduct cells synthesize TGFB1 and whether E2 and environmental oestrogens (EOEs; xenoestrogens and phytoestrogens) modulate its synthesis. Under basal conditions, bovine oviduct cells (OCs; oviduct epithelial cells and oviduct fibroblasts; 1:1 ratio) synthesized TGFB1. E2 concentration-dependent induced TGFB1 levels in OCs and these effects were mimicked by some, but not all EOEs (genistein, biochanin A and 4-hydroxy-2′,4′,6′-trichlorobiphenyl, 4-hydroxy-2′,4′,6′-dichlorobiphenyl); moreover, EOEs enhanced (P < 0.05) the stimulatory effects of E2 on TGFB1 synthesis. The OCs expressed oestrogen receptors alpha and beta and aryl hydrocarbon; moreover, co-treatment with ER antagonist ICI182780 blocked the stimulatory effects of E2 and EOEs on TGFB1 synthesis. Treatment with non-permeable E2-BSA failed to induce TGFB1, thereby ruling out the involvement of membrane ERs. Cycloheximide (protein synthesis inhibitor) blocked E2-induced TGFB1 synthesis providing evidence forde novosynthesis. The stimulatory effects of E2 and EOEs, were inhibited (P < 0.05) by MAPK inhibitor (PD98059), whereas intracellular-Ca2+chelator (BAPTA-AM) and adenylyl cyclase inhibitor (SQ22536) abrogated the effects of E2, but not EOEs, suggesting that post-ER effects of E2 and EOEs involve different pathways. Our results provide the first evidence that in OCs, E2 and EOEs stimulate TGFB1 synthesis via an ER-dependent pathway. Exposure of the oviduct to EOEs may result in continuous/sustained induction of TGFB1 levels in a non-cyclic fashion and may induce deleterious effects on reproduction.
Fabrication of Nanocomposite Scaffolds Including Metal Nanoparticles for Tissue Engineering Applications
Authors: Ayşen Aktürk  , Cagkan Alemdar  , Emre Ekmen  , İrem Keskin  , Nagihan Tunca  , Melek Erol Taygun  , Sadriye Kücükbayrak
Published: December 2017

This study presents the findings of a research on fabricating composite nanofibrous mats including silver and copper nanoparticles for tissue engineering applications. For this purpose, two different types of silver nanoparticles (soluble starch capped silver nanoparticles, sodium alginate capped silver nanoparticles) and two different types of copper nanoparticles (soluble starch capped copper nanoparticles, sodium alginate capped copper nanoparticles) were successfully incorporated into polyvinyl alcohol (PVA) fibers through electrospinning process. Characterization studies with x-ray diffraction (XRD), scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR), inductively coupled plasma spectrometer (ICP) were conducted to determine physical and structural properties of the obtained nanofiber mats. According to SEM analysis it was observed that interconnected and randomly-oriented nanofibers were successfully generated. Additionally, XRD and FTIR studies proved the existence of silver nanoparticles and hydroxapatite on the nanofiber mats immersed in simulated body fluid (SBF) for 7 days. The results indicated that long term silver ion release was achieved. Overall results showed that these nanofibrous mats can be good candidates as multifunctional scaffolds for tissue engineering applications.
Imposex en Crassilabrum crassilabrum (Neogastropoda: Muricidae) en la Costa Central del Perú
Authors: Rivas Rolf , Hurtado Soledad , Cornelio Dayanne , Mendoza Jorge , Guabloche Angélica , Iannacone José , Alvariño Lorena , Castañeda Luz
Published: December 2017

El imposex es un fenómeno que consiste en el desarrollo de caracteres sexuales masculinos sobre el sistema reproductivo de las hembras de caracoles gasterópodos por la contaminación por compuestos organoestañosos (OTs), como el tributil estaño (TBT). En el presente trabajo se evaluó el imposex en Crassilabrum crassilabrum Sowerby, 1834 (Neogastropoda: Muricidae) proveniente de la costa central del Perú, para lo cual se analizaron individuos colectados de la zona intermareal de Pucusana y Ancón, Lima. Se tomaron medidas biométricas y se calcularon los índices de imposex. El porcentaje de imposex en Ancón fue del 98% y en Pucusana de 100%. El índice de la longitud relativa del pene (RPLI) fue de 52.3% y 44.5% para Pucusana y Ancón, respectivamente. Una relación significativa fue encontrada entre la longitud de la conchilla y la longitud del pene en machos en ambas playas, pero no así en las hembras. Crassilabrum crassilabrum es un molusco con aplicación para emplearse como un bioindicador de componentes OTs en la costa central del Perú y en el Pacífico sudamericano.
Identificación de Hongos Micorrízicos en Plantaciones de Melina (Gmelina arborea Roxb) en el Trópico Húmedo Ecuatoriano
Authors: Meza B. Fabricio , Díaz O. Eduardo , Escobar T. Harold , Belezaca Pinargote Carlos , Cachipuendo C. Jesica , Meza B. Gary , López M. Francel , Meza B. Carlos , Meza B. Jessica , Cachipuendo C. Judith , Cabrera V. Rodrigo
Published: December 2017

El objetivo de esta investigación fue aislar e identificar hongos formadores de micorriza arbuscular en plantaciones de melina (Gmelina arborea Roxb). Se muestrearon dos plantaciones de melina de 1 y 3 años de edad, ubicadas en la zona central del trópico húmedo ecuatoriano, provincia de Los Ríos, cantón Valencia. Las muestras de suelo y raíces se recolectaron en la época seca entre junio y diciembre de 2015. Los resultados mostraron la presencia de los géneros de hongos micorrízicos arbusculares Glomus y Gigaspora. El género con mayor representatividad en cantidad de esporas por gramo de suelo fue Glomus. El mayor porcentaje de colonización micorrízica se presentó en las raíces de los árboles de 1 año de edad.
Congelación de Semen Equino Bajo Dos Esquemas de Adición de Dimetilformamida
Authors: Pérez Q. Daniel Domingo , Acosta L. Mariano , Restrepo B. Giovanni , Camacho Cesar , Pérez O. Jair
Published: December 2017

El objetivo del estudio fue evaluar la calidad posdescongelación de semen equino sometido a dos esquemas de adición de dimetilformamida (DMF) durante la congelación. Se obtuvo el semen de cinco caballos criollos colombianos, el cual fue sometido a dilución en medio INRA82® modificado con dos tratamientos de adición de DMF: T1, adición inmediata de 5% de DMF; T2, adición fraccionada de 5% de DMF (durante 10 min). El semen se empacó en pajillas de 0.5 ml que se sometieron a congelación convencional. La movilidad espermática, la vitalidad y la morfología de los espermatozoides se evaluaron pre- y posdilución. Adicionalmente, se realizó un análisis posdescongelación de la movilidad y de la cinética espermática mediante un sistema computarizado, y la evaluación de la integridad de la membrana plasmática por la prueba hipoosmótica (HOS). Se observó una menor reducción de la movilidad espermática por la adición inmediata de DMF (T1) antes y después de la congelación, en comparación con la adición fraccionada del crioprotector (T2) (p<0.05). Se encontraron valores superiores de movilidad total y progresiva posdescongelación para T1, mientras el índice de rectitud (STR) fue mayor para T2 (p<0.05). Se concluye que la adición inmediata de DMF durante la congelación, provee una mayor protección de la movilidad espermática posdescongelación del semen equino, respecto a la adición fraccionada de este crioprotector.
Congelación de Semen Epididimal Canino con Yema de Huevo Centrifugada
Authors: Restrepo B. Giovanni , Madrid R. Carlos Andrés , Prieto R. Laura , Duque C. Juan Esteban , Usuga S. Alexandra
Published: December 2017

El objetivo de esta investigación fue evaluar el uso de yema de huevo centrifugada (YHC) en la congelación de espermatozoides epididimales caninos. Se obtuvieron los complejos testículo-epidídimo (CTE) después de la orquiectomía de 20 perros domésticos (Canis familiaris) de varias razas y en un rango de edad entre 1 y 6 años. Se diseccionó la cola del epidídimo y se obtuvieron los espermatozoides mediante la técnica de flujo retrógrado. Los espermatozoides obtenidos se dividieron en dos alícuotas y se diluyeron en Triladyl® suplementado con YHC al 10% (YHC10) o YHC al 20% (YHC20). Se realizó la congelación del semen mediante un protocolo convencional con vapores de nitrógeno líquido en pajillas para 0.5 ml. Se utilizó un sistema computarizado para evaluar la movilidad, la cinética y la concentración de los espermatozoides. Adicionalmente, se evaluaron posdescongelación la vitalidad espermática (VE), la morfología anormal (MA) y la integridad de la membrana plasmática (HOS). El análisis estadístico se realizó mediante el ajuste de modelos lineales generalizados (GLM) y la comparación de medias por la prueba de Tukey. Para YHC10 se encontraron valores superiores para las velocidades lineal (VSL), curvilínea (VCL) y media (VAP) en comparación con YHC20 (p<0.05). No se encontraron diferencias relacionadas con la concentración de YHC para otros parámetros de movilidad y cinética espermática, así como para VE, MA y HOS. Se concluye que la YHC puede ser utilizada como suplemento para la congelación de semen epididimal canino. Adicionalmente, cuando la YHC se utiliza en una proporción del 10% del diluyente, favorece la cinética espermática posdescongelación.
Características de la Fibra de Alpaca Huacaya de Cotaruse, Apurímac, Perú
Authors: Machaca Machaca V. , Bustinza Choque A.V. , Corredor Arizapana F.A. , Paucara Ocsa V. , Quispe Peña E.E. , Machaca Machaca R.
Published: December 2017

El objetivo del estudio fue establecer el perfil de las principales características físicas de la fibra de alpaca que pueden servir para su mejor comercialización y para fines de mejoramiento genético. Se hicieron mediciones de 145 muestras de colores blanco, intermedio y obscuro pertenecientes a alpacas de cinco comunidades del distrito de Cotaruse, Apurímac, Perú, utilizando el equipo ODFA 2000 para determinar el diámetro de fibra (DF), el coeficiente de variación (CV[DF]), el factor de confort (FC) y el índice de curvatura (IC). Se estimaron los efectos de la comunidad, el sexo, edad, color de la fibra y sitio de muestreo sobre el DF, CV(DF), FC e IC y la relación entre ellos. El promedio del diámetro de fibra (MDF) estuvo influenciado por la edad (p<0.01) (valores entre 21.61 y 24.32 ìm), por color de fibra (22.30, 23.81 y 26.69 ìm para blanco, intermedio y obscuro, respectivamente) y por comunidad (de 21.9 ìm para Iscahuaca a 24.2 ìm para San Miguel de Mestizas) y por sexo (p<0.05), siendo la fibra de las hembras 1 ìm más fina que la de los machos; sin embargo, no hubo diferencias significativas por la zona corporal de la toma de la muestra. El CV(DF) mostró diferencias significativas por efecto de la edad (p<0.01) y por sexo y sitio de muestreo (p<0.05), pero sin diferencias por color y comunidad. El FC tuvo diferencias significativas (p<0.01) por efecto de la comunidad y color de la fibra, así como por edad, sexo y sitio de muestreo (p<0.05). El IC tuvo diferencias significativas debido a la comunidad (p<0.01), edad, sexo y color (p<0.05), pero no por el sitio de muestreo. La MDF presentó una alta y negativa correlación con FC (r=-0.99) e IC (r=-0.61) y la FC presentó una correlación positiva con IC (r=0.62). No se encontró una definición clara del sitio apropiado para el muestreo del vellón de alpaca, pero se puede realizar a la edad de 1 año. Las alpacas del distrito de Cotaruse, Apurímac, indistintamente del color del vellón, producen una buena calidad de fibra y hay un gran potencial de variabilidad para su mejoramiento genético.
Lesiones Anatomopatológicas Producidas por Dosis Prolongadas de Fenilbutazona en una Potranca de Carrera
Authors: Choez A. Katherine , Chavera C. Alfonso
Published: December 2017

El presente trabajo describe el caso de una potranca de dos años que desarrolló graves lesiones gastrointestinales después de recibir dosis prolongadas de fenilbutazona al tratar una fractura en el miembro anterior derecho. Los hallazgos de necropsia revelaron una severa gastritis catarro- hemorrágico-ulcerativa difusa aguda y edema submucoso y severa enterocolitis mucohemorrágica difusa aguda y esplenomegalia con severa congestión pasiva aguda. La causa de muerte se debió a un paro cardio-respiratorio y coagulopatía con disturbio circulatorio y proceso ulcerogénico gástrico asociado a shock iatrogénico por fenilbutazona.
Detección de Anticuerpos contra Ehrlichia spp en Propietarios de Caninos Domésticos con Ehrlichiosis
Authors: Gómez M. Beatriz , Li E. Olga , Manchego S. Alberto , Hoyos S. Luis , Suárez A. Francisco fsuareza@unmsm.edu.pe
Published: December 2017

Se determinó la seropositividad contra Ehrlichia chaffeensis en propietarios de perros con antecedentes de ehrlichiosis mediante la prueba de inmunofluorescencia indirecta (IFI) y su asociación con el género, edad, exposición a garrapatas y nivel de contacto con los perros. El estudio se realizó entre enero de 2009 y diciembre de 2009, donde se evaluaron 95 personas de Lima Metropolitana (Perú), sin distinción de género, edad o condición socioeconómica y cuyos perros tenían historia de ehrlichiosis menor de un año. El 31.6% (30/95) presentaron anticuerpos contra E. chaffeensis. El 38.7% (24/62) de los individuos menores a 40 años y el 18.2% (6/33) de los individuos mayores a 40 años resultó ser seropositivo a E. chaffeensis (p<0.05). El 29.4% de varones y el 32.7% de mujeres resultaron seropositivos a E. chaffeensis (p<0.05). Además, el 40.0 y 8.0% de las personas expuestas y no expuestas a artrópodos presentaron seropositividad a este patógeno.
QUALITY OF BOVINE PREIMPLANTATION EMBRYOS IN RELATION TO CATTLE BREED
Authors: V. Makarevich Alexander , Kubovičova Elena , Chrenek Peter
Published: December 2017

The aim of this study was to compare quality of bovine preimplantation embryos recovered from cows of three cattle breeds bred in Slovakia: Holstein (n= 38), Pinzgau (n= 28) and Slovak Pied (n= 53). Total number of embryo cells (DAPI-stained nuclei) and incidence of dead cells (TUNEL-positive nuclei) were counted in total of 563 bovine blastocysts collected from three breeds under the Leica fluorescent microscope. This study was done in 12 experiments performed during seven years. The lowest total number of embryo cells was counted in Pinzgau cattle (107 ± 8.5) and the highest – in Holstein cows (121 ± 5.5), whilst in Slovak Pied cows the embryo cell number was at moderate value (112 ± 6.4). The highest dead cell index (%) was calculated in embryos from Pinzgau cattle (6.22 ± 0.57), whilst Holstein and Slovak Pied embryos showed almost similar values (5.72 ± 0.34 and 5.31 ± 0.49, resp.). However, in both cases these differences among the breeds were statistically insignificant. In conclusion, present study did now reveal an influence of cattle breed on the quality of bovine preimplantation embryos. These findings may have an importance at the realization of genetic resource preservation program in Slovakia.
INFLUENCE OF GLUCOSE AND L-ASPARAGINE CONCENTRATIONS ON L-ASPARAGINASE PRODUCTION BY ENDOPHYTIC FUNGI
Authors: Yng Chow Yiing , Su Yien Ting Adeline
Published: December 2017

In this study, five L-asparaginase–producing endophytes (ODL4, PBS1, PBL13, CCL1, MKS1) were evaluated for their L-asparaginase production when cultivated in various glucose (0, 0.5, 1, 2, 3, 4, 5 %) and L-asparagine (0, 5, 10, 20, 30, 40, 50 mM) concentrations over a period of 20 days. L-asparaginase production was quantified via Nesslerization and expressed as L-asparaginase activities. Results revealed supplementation with nitrogen-source (L-asparagine) induced higher L-asparaginase activities compared to carbon-source (glucose), with means of 0.106 compared to 0.062 µM mL-1 min-1, respectively. The optimum concentration of L-asparagine was 30 mM. On the contrary, optimum levels for glucose was inconclusive. Among the isolates, isolate MKS1 produced the highest L-asparaginase activities when supplemented with 5% glucose and 10 mM asparagine. This study revealed that nitrogen-based source (L-asparagine) is more effective than carbon-source (glucose) in inducing L-asparaginase activities.
BIOFILM PRODUCTION BY Staphylococcus sp ON STAINLESS STEEL CHIPS IN CONTACT WITH BRAZILIAN MINAS CHEESE HOMOGENATES AND BHI BROTH UNDER DIFFERENT EXTRINSIC FACTORS
Authors: C.C. Silva NathÃ¡lia , C.R. Bonsaglia Ãrika , Fernandes Júnior Ary , T. Hernandes Rodrigo , C.F. Pantoja JosÃ© , L.M. Rall Vera
Published: December 2017

The aim of this study was to evaluate biofilm production by Staphylococcus sp on stainless steel using brain heart infusion (BHI) broth and Minas Frescal cheese broth, at different levels of contamination and under different temperatures, atmospheres. The cheeses were classified according to their microbiota. It was evaluated biofilm formation on stainless steel chips at 8, 15 and 35°C after 48, 72 and 96 h under different conditions. Both Staphylococcus isolates formed biofilm on stainless steel chip in BHI broth and cheese broth, under all temperatures and atmospheres studied. In conclusion, BHI broth and Brazilian Minas cheese homogenate performed equally well for biofilm formation tests using Staphylococcus strains, and S. aureus can produce biofilm on stainless steel at 8ºC, which may be a concern for the dairy industry. Finally, it is important to consider that S. aureus can be a good competitor, depending on the nature of the microbiota.
COMPARATIVE ANALYSIS OF Mal d 1.03A EXPRESSED TRANSCRIPTS IN PULP OF APPLE CULTIVARS GOLDEN AND SPARTAN
Authors: Knoteková Ä½udmila , Žiarovská Jana
Published: December 2017

Apples are one of the oldest, most desirable and most consumed fruit in the world and in our agroclimatic conditions apples are the fruit that are also in the winter mostly consumed fresh. As apples are available all the year, they are a very important source of secondary plant metabolites. On the other hand, apples do not provide only benefits and belong to the fruit that cause an allergic reaction in a sensitized the population. Mal d 1 protein, one of the apple allergens, belongs to the PR proteins and is encoded by multiple isoforms in the genome of Malus domestica Borkh. The aim of the study was to compare the level of expression of Mal d 1.03A isoform in the pulps of apple varieties Golden and Spartan during the ripening. Expression analysis of Mal d 1.03A allergen were performed in pulps from a total of six ripening stages ranged from the early up to the physiological maturity at approximately monthly intervals. Assessment of the level of amplification of transcripts of Mal d 1.03A allergen was performed by software comparing them to a well-defined value of 1000 ng × μl-1. In the comparing analysis, the levels of amplified transcripts of Mal d 1.03A were more balanced in the first stages of ripening in the variety Spartan. A high increasing of the amount of transcripts were obtained in the first stages of ripening in the variety Golden. In Continual increasing of Mal d 1.03A transcripts was observed the middle ripening stage for both varieties. At the end of the ripening a different situation was observed in analysed apple varieties. The expression of Mal d 1.03A allergen was balanced for the variety Golden but a high increase was observed at the end of the ripening for the variety Spartan.
PROTECTION AND SUSTAINABILITY OF ANIMAL GENETIC RESOURCES FOR ENSURING THE PRODUCTION OF QUALITY DOMESTIC FOOD
Authors: Chrenek Peter , Kubovičová Elena , Makarevich Alexander
Published: December 2017

Rapid rise in population, economic growth and urbanization are important factors, which can affect the current and future quality of life and food security. An enormous pressure to streamline animal breeding and production have led to the rapid erosion of animal genetic diversity. The importance of animal genetic resource protection emerges from biological, economic, social, cultural and environmental needs of the country. Animal genetic resources contribute to the development of good food quality and food products, new medicines and manufactured goods, as well as other important goods and services. This will ensure that these resources will be fully used to respond to current food security and that they will continue to contribute to human development and well-being. The situation in protection of animal genetic resources in the Slovak Republic is not satisfactory, therefore, it is very important to pay attention to the management of certain breeds and to its conservation. Also, the legal base is needed to ensure the long-term maintaining of the endangered breeds.
INFLUENCE OF MACERATION AND ADDED FLAVOR – RELEASING ENZYME ON THE AROMATIC COMPOSITION OF WHITE WINES
Authors: Dimitrov Dimitar , Yoncheva Tatyana , Haygarov Vanyo
Published: December 2017

Study on the impact of maceration and maceration with the addition of flavor-releasing enzyme on the aromatic composition of white wines from grapes grown in Central Northern Bulgaria was conducted. White wines from varieties Dimyat, Vrachanski Muscat, Aligote, Muscat Ottonel and Plevenska Rosa were examined. The research was performed at the Institute of Viticulture and Enology, Pleven, Bulgaria in the period 2015 – 2016 year. In the tested wines were identified twenty four volatile compounds related to the basic aromatic groups – esters, higher alcohols, terpenes. Presence of acetaldehyde and methyl alcohol was found. The study demonstrated the following trend: the use of oenological practices - maceration and maceration with the addition of flavor-releasing enzyme have a positive influence on the aromatic composition of wines, increasing the total content of esters, higher alcohols, and terpenes. The amount of methyl alcohol established in part of the wines was within the range 0.007 - 0.030% of the total alcohol content, typical characteristics for white wines composition. The established content of acetaldehyde in the wines (53.80 - 191.00 mg/dm3) was three times lower than the maximum limit. The comprehensive analysis of the results leads to the conclusion that the application of oenological practices - maceration (12h) and maceration (12h) with the addition of flavor-releasing enzyme in the production of white wines from studied varieties results in improved overall aromatic composition and increase their chemical complexity.
BIOCHEMICAL CHARACTERIZATION OF ANTI-METHICILLIN RESISTANT S. aureus PROTEIN (P-80) FROM MARINE Pseudoalteromonas
Authors: Bautista Secun Jorge , Hernández Santoyo Alejandra , Monteón Padilla VÃ­ctor , Hernández Vázquez Oscar , López Alcántara Ruth
Published: December 2017

The marine Pseudoalteromonas are recognized as major active metabolite producers against different biological targets. These compounds include cyclic peptides, toxic proteins or enzymes, among others. In previous studies, we isolated a protein named P-80 from Pseudoalteromonas sp with antibiotic activity against S. aureus, and proteolytic action. In the present work, we studied properties such as structure and the function of P-80. We performed different assays, including MALDI-TOF mass spectrometry, antibacterial activity by the standard disc diffusion, pH and temperature stability, cytotoxicity in vitro and finally the effect of metal ions and protease inhibitors on antimicrobial activity. The purified P-80 showed a molecular mass of 78.5 kDa as determined by MALDI-TOF mass spectrometry. It was highly active over pH range of 7.0 to 9.2, and remained stable over a pH range of 7.0 to 8.0 although its activity was reduced at pH 9.2 (only 55% was retained). This protein showed quite a good antibacterial activity over a broad temperature range (> 65% of activity is retained between -20 and 70 ºC). The inhibitor tested such as EDTA, EGTA, PMSF, DTT and TLCK (at 5 mM) had no effect on the antibacterial activity. Whereas Mn2+ ions enhanced the antibiotic activity but Zn2+, Fe2+, Mg2+, Ca2+, Cu2+ and Co2+ ions had no effect. In conclusion, the P-80 protein remained 55% and 65 % of its antimicrobial activity against Staphylococcus aureus at pH 9.0 and 70 ºC respectively, suggesting high stability compared to other marine antibacterial proteins indicating intrinsic features that could enable commercial applications.
MOLECULAR PHYLOGENY OF SELATIUM BROCKII AND NANOSESARMA MINUTUM GRAPSID CRABS INFERRED FROM COI GENE
Authors: Rajan Durairaj Karthick , Fredrick Wilson Sylvester , Samuthirapandian Ravichandran
Published: December 2017

The phylogenetic relationship of the two grapsid crabs Selatium brockii and Nanosesarma minutum collected from mangrove creeks have been studied corresponding to the mitochondrial genome cytochrome oxidase subunit I (COI) gene. Genomic DNA was isolated from the claw muscle of the two crabs Selatium brockii and Nanosesarma minutum. The COI gene of the crabs was amplified and sequenced. The phylogenetic tree was constructed from the COI gene sequence. Present findings show that the genetic relationship between these two species to be 74% and both the species show 99% similarity to Parasesarma erythrodactyla strain MSCAS9 (KX018517), a red handed shore crab found in the mangrove creeks. COI gene could be a useful molecular marker to determine the monophyly of a crustacean family. No earlier reports were available on the molecular phylogeny of currently studied species. Increase in the taxon sampling of COI gene sequences from different groups could be a promising methodological resolution for determining the genetic divergence of the species.
INFLUENCE OF CULTURAL CONDITIONS FOR EXTRACELLULAR LIPASE PRODUCTION BY A HALOTOLERANT BACTERIUM, BACILLUS sp. ORS4
Authors: Biswas Jhuma , Barman Rupa , K. Paul A.
Published: December 2017

During the course of survey of halotolerant bacteria from saline environments, 17 bacterial cultures were isolated in pure form from the multi-pond solar salterns of Odisha and West Bengal, India. The isolates were screened for their lipase production capability on basal MH-Tween 80 agar medium containing 10% NaCl and the isolate ORS4 having potential for high lipase production, the most potent lipolytic isolate ORS4 was selected for further detailed studies. The ORS4 isolate showed most similarity to Bacillus vallismortis from genus Bacillus on behalf of the morphological, physiological and biochemical characteristics along with 16S rRNA gene sequence analysis. Time course of growth and lipase production by the isolate ORS4 showed maximum enzyme activity (35.79 U/ml) during the stationary phase of growth. Moreover, the optimum conditions for lipase production by the isolate in basal MH medium involved 1% Tween 80, 5% NaCl, 1% glucose, 1% tryptone with an inoculum density of 1012 cells/ml. The partially purified extracellular lipase of Bacillus sp. ORS4 showed tolerance to alkaline pH along with high salinity and temperature suggesting its potential for biotechnological applications in saline environments.
EXTRACTION AND IDENTIFICATION OF ESSENTIAL OIL FROM CINNAMOMUM ZEYLANICUM BARKS AND STUDY THE ANTIBACTERIAL ACTIVITY
Authors: F. Al-fekaiki Dhia , Kareem Niamah Alaa , Thyab Gddoa Al-Sahlany Shayma
Published: December 2017

This study aimed to identify the essential oil compound from Cinnamomum zeylanicum barks by using GC-MS analysis and evaluate essential oil inhibition effects against Listeria monocytogenes, Escherichia coli, Enterobacter aerogenes, Pseudomonas erogenous and Staphylococcus aureus by using agar diffusion method and studied kill-time for this bacterium. The yield of Cinnamomum zeylanicum essential oil was 5%. Cinnamaldehyde 57.83% was the main compound in the essential oil extract, followed by cyclohexane carboxylic acid 9.29% and 6-octadecenoic acid 8.41%. Gram-positive and Gram-negative bacteria were inhibited growth after addition 6, 12 and 18 μL of essential oil. Zone diameter of inhibition ranged from 17.00-30.30 mm for bacterial test. A concentration of 10, 20 and
30 μL.mL-1 of the essential oil caused a rapid and steady decline in the number of viable cells from 2 to 5 Log. cycles of all strains during 24 hours.
Assessment of adrenocortical reserve capacity and inflammatory parameters in critically ill dogs
Authors: Csöndes Judit , Fábián Ibolya , Szabó Bernadett , Máthé Ákos , Vajdovich Péter
Published: December 2017

Inflammatory markers and adrenocorticotropic hormone (ACTH) stimulation test results may help us recognise critically ill dogs with poor disease outcome. Systemic inflammatory response syndrome (SIRS) criteria, the fast version of the Acute Patient Physiologic and Laboratory Evaluation Score (APPLEfast), complete blood count, albumin and C-reactive protein (CRP) levels, baseline and stimulated cortisol levels and Δcortisol value were recorded in 50 client-owned dogs admitted to the Small Animal Hospital of the University of Veterinary Medicine Budapest with various inflammatory or neoplastic conditions. Increasing APPLEfast score was associated with a decreasing chance of survival (P = 0.0420). The Δcortisol value was significantly higher in SIRS dogs than in non-SIRS dogs (mean ± SD ΔcortisolSIRS: 342.5 ± 273.96; mean ± SD Δcortisolnon-SIRS: 175.3 ± 150.35; P = 0.0443). Elevated baseline or stimulated cortisol levels were associated with a higher chance of non-survival (P = 0.0135 and P = 0.0311, respectively). These data indicate that pathologically higher baseline and stimulated cortisol levels represent an exaggerated stress response in critically ill dogs, which is negatively associated with survival.
Prevalence of Trypanosoma cruzi and organ alterations in Virginia opossums (Didelphis virginiana) from western Mexico – short communication
Authors: Carnevali Vincenzo , Nogueda-Torres Benjamín , Villagrán-Herrera María E. , De Diego-Cabrera José A. , Rocha-Chávez Gonzalo , Martínez-Ibarra José A.
Published: December 2017

Small populations of Virginia opossum (Didelphis virginiana) in western Mexico are endangered by hunting and natural predators as well as by different kinds of diseases. After two serological analyses using Serodia® latex particle agglutination and indirect haemagglutination (IHA) tests, 35 (53.03%) of 66 collected opossums in two small towns in western Mexico were positive for the presence of Trypanosoma cruzi. Twenty-eight of the 35 seropositive opossums had pathological lesions: 11 had changes in only one organ, 13 in two organs, and four had pathological changes in three organs. Splenomegaly was the most common finding in the examined opossums, followed by hepatomegaly. These potentially fatal pathological changes could contribute to the scarcity of the opossum population, even leading to the extinction of this species in western Mexico.
Effect of storage tube material and resveratrol during liquid storage of matured bovine oocytes on subsequent development
Authors: Suttirojpattana Tayita , Somfai Tamás , Matoba Satoko , Nagai Takashi , Parnpai Rangsun , Geshi Masaya
Published: December 2017

This study determined the optimum storage vessel and the effects of resveratrol for the storage of in vitro matured (IVM) bovine oocytes. After IVM, the oocytes were kept in a Hepes-buffered medium at 25 °C for 20 h in different containers including Eppendorf tubes (ET) made of polypropylene (PP) and polystyrene (PS), and tissue culture tubes (TCT) made of PP, PS, and glass. Then oocytes were subjected to IVF and subsequent in vitro embryo development was compared among the groups and to that of a control group without storage. The percentage of blastocyst development in the control group was significantly higher than in the stored groups (P < 0.05). Among oocytes stored in TCT, the percentage of blastocyst development of oocytes stored in glass TCT was significantly higher than that of oocytes stored in PP and PS TCT (P < 0.05); however, it did not differ from that of oocytes stored in ET. The quality of blastocysts did not differ among the control and stored groups. Embryo development was not affected when 0.1, 1 or 10 μM resveratrol was added to the medium during oocyte storage. In conclusion, glass tubes were optimal for oocyte storage and resveratrol did not improve the development of stored oocytes.
Transurethral urinary bladder eversion and prolapse in a castrated male pet rabbit
Authors: Szabo Zoltan
Published: December 2017

A 7-year-old castrated male rabbit was presented with a red oedematous mass at the prepuce. The tissue was identified as the urinary bladder, and the condition was diagnosed as complete transurethral urinary bladder eversion. Exploratory laparotomy was performed, the prolapse was successfully reduced and the bladder was secured to the body wall with cystopexy. The surgery was successful and the bladder remained in place without complications until the time of this report (three years after surgery). Transurethral bladder prolapse is a very rare condition previously reported only in women, mares, cows, bitches, queens, and rabbit does. The case herein is the first reported case of transurethral bladder prolapse in a male of any species.
EFFECT OF THE CHANGE OF CLIMATE INDICATORS ON AGRICULTURAL YIELDS IN SON LA
Authors: Hung Nguyen The , Quyen Nguyen Huu
Published: December 2017

Due to climate change, the agro-climatic indicators in Son La province has changed in the following trends: (i) the total heat in the Winter - Spring crop and the Summer crop as well as the total annual heat have increased; (ii) The amount of time in a year with temperature below 20 degrees has shortened; the amount of time in a year with temperatures above 25 degrees has lengthened; (iii) the absolute minimum temperature tends to increase rapidly; (iv) the rainfall in Winter - Spring crop has increased slightly whereas the season rainfall and total annual rainfall tend to decrease. The climatic factors in Son La province obviously affect rice yield in the Winter - Spring crop, the Summer crop as well as corn and soybeans. However, compared with other crops, soybean yield is less related to climatic factors. There are major differences in climate characteristics between the high and the low crops yield year. However, these differences depend on the type of the crop and the harvest season.
A RESEARCH ON THE EFFECT OF CUTTING PARAMETERS ON CUTTING FORCE IN FLAT GRINDING USING SEGMENTED GRINDING WHEEL
Authors: Phuong Nguyen Thi , Nguyen Giang Phuong Thi , Nguyen Dong Tien
Published: December 2017

This article represents the result of a research on the impact of cutting parameters including the number of segments, cutting depth and feed rate on cutting force P when grind SKD11 harden steel using bevel segment grinding wheel using Taguchi method and analysis of variance (ANOVA). The result release the information of the relationship between the number of segments, cutting depth and feed rate and its interactions with cutting force. Local optimal value of cutting force can also be determined with minimal cutting force and low surface roughness when machining the SKD11 harden steel using bevel segment grinding wheel.
ELECTRODEPOSITION OF HYDROXYAPATITE/FUNCTIONALIZED CARBON NANOTUBES (HAp/fCNTs) COATINGS ON THE SURFACE OF 316L STAINLESS STEEL
Authors: Thom Nguyen Thi , Nam Pham Thi , Phuong Nguyen Thu , Hong Cao Thi , Trang Nguyen Van , Xuyen Nguyen Thi , Mai Thanh Dinh Thi
Published: December 2017

The coatings of hydroxyapatite/functionalized carbon nanotubes (HAp/fCNTs) were successfully synthesized on the surface of 316L stainless steel (316L SS) by electrodeposition. To improve the dispersion of CNTs into the electrolyte, CNTs were functionalized in the mixture of 67 % HNO3 and 98 % H2SO4 before electrodeposition coatings to form fCNTs. Different amounts of fCNTs were  incorporated into the apatite coatings by adding fCNTs into the  electrolyte solution. In this study, Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Thermal Gravimetric Analysis (TGA) and mechanical techniques were used to analyze characteristic of fCNTs and the HAp/fCNTs coatings. The FTIR spectra of CNTs before and after acid treated process show that CNTs are functionalized by chemically method with carboxylic acid groups (–COOH) on their surface. The SEM images confirm that the functionalized process by the acid mixture does not change the morphology of CNTs. The nanocomposite coatings contain single phase of HAp and CNTs with the coating thickness of 6.9 µm and the amount of fCNTs in the coatings is 7.25 %. With 0.5 g/L of fCNTs in the electrolyte solution, the solubility of the coating decreases in comparision with pure HAp coatings.
WATER-SOLUBLE COMPONENTS OF Ancistrocladus cochinchinensis
Authors: Lien Le Quynh , Tuan Anh Hoang Le , Cuc Nguyen Thi , Linh Tran My , Giang Vu Huong , Ban Ninh Khac , Mai Nguyen Chi , Hung Trieu Quy , Hai Yen Pham Thi , Kiem Phan Van
Published: December 2017

Using various chromatography methods, a saponin, ginsenoside Rg1 (1), and three phenolic glycosides, tortoside A (2), phlorizin (3), and 4-hydroxy-2-methoxyphenyl-6-O-syringyl-b-D-glucopyranoside (4) were isolated from the water-soluble fraction of Ancistrocladus cochinchinensis. Their structures were elucidated by 1D- and 2D-NMR spectroscopic analyses and comparison with those reported in the literature. Compound 1 was reported from A. cochinchinensis for the first time.
OPTIMIZATION OF THE ENZYME ASSISTED EXTRACTION OF ESSENTIAL OIL FROM THE LEAVES AND BRANCHES OF CINNAMOMUM CASSIA USING BOX-WILSON METHOD
Authors: Bich Hoang Thi , Huong Le Mai , Chien Nguyen Quyet , Thuy Dinh Thi Thu , Thanh Le Tat , Sy Do Trung , Hang Le Thi Thuy , Hai Pham Hong
Published: December 2017

The process of enzyme assisted extraction of essential oil from the leaves and branches of the Vietnamese aromatic plant Cinnamomum cassia was studied and optimized using a Box-Wilson central composite design consisting of 05 independent variables (pH, temperature T, time τ, concentration of the enzyme Laccase, and concentration of the enzyme Cellic Htec2) and two dependent variables (reducing sugar and yield of essential oil). Second-order polynomial equations were obtained for the responses, which fitted well with the experimental data. Optimal conditions for oil yield were found at pH = 5.2; T = 440C; τ = 5h30'; Laccase = 0.42 ml/g, and Cellic Htec2 = 1.15%. The experimental value (0.982% oil yield) was close to the predicted value (0.978%). The application of enzyme assisted extraction  in combination with optimization using response surface methodology substantially improved the oil yield as compared with traditional method.
Короткие уникальные последовательности в бактериальных геномах как штамм- и видоспецифические маркеры
Authors: Панюков В.В. , Panyukov V.V.
Published: December 2017

The paper presents a new approach for phylotyping that can be potentially used for pure cultures and for mixed bacterial populations. It is based
on the use of short unique nucleotide sequences (k-mers) that are present in the genomes of all strains of the same species
and are absent in bacterial genomes of other taxonomic groups. We show that the number of such sequences depends on the percentage bias towards A/T
or G/C base pairs, increasing for genomes with approximately equal composition. We found that the largest contribution to the set of primarily
unique sequences is given by 16–17-mers, while sigmoidal curves reflecting the dependence of the number of unique sequences on the length of
k-mers showed the maximum slope increment for k = 17, 18. Unique sequences of
the length 16–18 bases can therefore be offered as potential markers. Comparing the sets of unique k-mers in the genomes
of four Enterobacter strains, we estimated the level of their intraspecies stability and interspecies plasticity. As a result, we suggest
discriminatory subsets as stencils for phylotyping, thereby increasing the list of genotyping markers with signatures of the new type.
THE ETHNIC DIMENSIONS IN SOCIAL MOVEMENTS*
Authors: Oliver Pamela
Published: December 2017

This article draws on work in the social construction of race and ethnicity to explain why racial/ethnic divisions are so often axes of domination and why these divisions are central to social movements. Racial/ethnic groups are constructed in political processes that are intertwined with state formation and social movements. Processes of state formation and collective action create racial/ethnic groups, define majorities and minorities, and create racial/ethnic structures of domination. Physical and social segregation in tandem with intergenerational inheritance creates network cliquing that reinforces group boundaries, group differences, and group interests. Movements by members of dominant racial/ethnic majorities differ from movements by members of subordinate racial/ethnic minorities in key ways, including access to democratic processes for achieving group goals, experience of repression, need for allies, identity construction, processes of consciousness raising, and bases of mobilization. These “ethnic dimensions” matter for all social movements.
Selective Targeting of T and B Cell Populations by Alemtuzumab in the Treatment of Multiple Sclerosis
Authors: Grigoriadis Nikolaos C ,   AHEPA University Hospital, Thessaloniki, Greece
Published: December 2017

Upstream targeting of both T and B cells is a rational therapeutic approach in multiple sclerosis (MS) in view of how both cell types and the interaction between them contribute to MS pathophysiology. This article will discuss this new way of thinking in MS: the targeting of both T and B cells, with a focus on the recently developed therapy, alemtuzumab (Lemtrada®, Genzyme, UK). Alemtuzumab depletes T and B lymphocytes, mainly via complement-dependent cytolysis and antibody-dependent cytolysis; depletion of B cells is not an enduring effect compared with the depletion of T cells. After dosing, CD4+ and CD8+ T cells and CD19 B cells decrease initially but increase over the following 11 months. During repopulation after alemtuzumab treatment, there is a shift in the relative proportions of T cell and B cell subsets whereby proportions of regulatory T cells and memory-phenotype T cells are increased and the proportion of naive T cells is decreased. A switch from a pro- to an anti-inflammatory phenotype and cytokine profile caused by alemtuzumab may underpin the long-lasting suppression of MS activity that has been observed in clinical trials. Alemtuzumab treatment is also associated with a consistently good safety and tolerability profile. Further, alemtuzumab appears to promote neurorehabilitation by improving measures of physical functioning, disability, measures of quality of life, and brain volume loss. Alemtuzumab therefore has the potential to reduce disease burden and improve substantially the prognosis for patients with MS.