bluuebunny/crossref_metadata_2025 huggingface dataset:
Getting familiar with Teaching Games for Understanding – A qualitative experiment with German and Swiss teachers
Authors: König S. , Baumberger J. , Bislin S.
Published: January 2022

The professionalization of teachers is one central topic in both educational sciences and subject didactics. Within this debate pedagogical content knowledge (PCK) has turned out to be a core component of professional competence. Consequently, PCK must be a primary task for teacher education. In Physical Education teaching games has been a conflicting issue in sport didactics for more than fifty years, especially considered from the perspective of game vs. skill orientation. In this case, the concept of TGfU has proved as an adequate and effective conception; thus, it can be assumed that this approach should be integrated into the education of PE teachers. This article deals with the issue of teaching TGfU to preservice teachers in Germany and Switzerland. We first deal with a framework for teacher education and its main target dimensions. Second, we present TGfU as a didactical conception for teaching sport games and its possibilities of implementing quality dimensions. Third, we present a study in which a qualitative experiment was conducted. It aimed at teaching the concept of TGfU to a variety of teachers over nine months. Some ideas and recommendations for teacher education round off the article.
Trauma-sensitive Physical education teacher education
Authors: Subramaniam P. R. , Wuest D. A.
Published: January 2022

Trauma is an epidemic and a public health crisis affecting schools and institutions of higher education globally. Childhood trauma has detrimental impacts on the brain, learning, and socio-moral development into adulthood. Trauma is a mental health as well as an educational problem that poses a serious threat to the academic achievement of students. Public schools have made some progress to become more trauma-sensitive to help build resiliency in students impacted by trauma, but institutions of higher education are lagging in the area of trauma-sensitivity. Physical education teacher preparation programs need a paradigm shift to address trauma with the COVID-19 pandemic posing new challenges. Preparing preservice physical education teachers to be trauma-informed lays the foundation for future teachers to mitigate trauma in schools. Such an effort will align physical education teacher education with public schools in addressing trauma and closing the trauma-sensitivity loop.
Health-Related Quality of Life and Related Factors in Patients with Fibromyalgia: A Cross-sectional Study
Authors: Jafari Fatemeh , Mobini Maryam , Moradi Siavash , Dashti Dargahloo Saeed , Ghafour Ideh , Elyasi Forouzan
Published: January 2022

Background: Fibromyalgia syndrome (FMS) is a chronic musculoskeletal condition, which can reduce the quality of life (QoL). Objectives: This study aimed to evaluate the impact of FMS on health-related quality of life (HRQoL) and the possible related factors such as sociodemographic and psychological variables. Methods: In this cross-sectional study, 150 women patients with FMS were included who visited a psychosomatic clinic at a referral center in northern Iran. The samples were selected in a consecutive procedure from April 2019 to March 2019. Sociodemographic variables, HRQoL score [36-Item Short Form (SF-36) Health Survey], and the Pittsburgh Sleep Quality Index (PSQI) were recorded. Data were analyzed using the SPSS Statistics software (ver. 24). The quantitative data were reported as mean ± SD, and the qualitative variables were presented as frequency and percentage. Also, analysis was performed using analytical tests such as chi-square test (χ2). P < 0.05 was considered to be significant. Results: The mean (SD) age was 42.35 (11.73) years. The mean physical and mental health scores were 33.45 (16.22) and 48.99 (18.19), respectively. Moreover, 88% of patients had moderate-to-severe sleep disorder, and those who did not, had a better physical and mental health status [42.7 (14.1) vs. 32.2 (16.1), P-value = 0.009 and 62.2 (16.3) vs. 47.2 (17.7), P-value = 0.001, respectively]. History of depression according to self-reporting was associated with worse mental health subscale scores [44.4 (16.8 Vs. 52.4 (18.5), P-value = 0.007], and lower scores in social functioning and emotional well-being (P-value = 0.012, P-value = 0.001, respectively). Being postmenopausal was associated with a lower physical health quality (P-value = 0.049). Body Mass Index (BMI) reversely correlated with HRQoL subscales (r = -0.163, P-value = 0.046), but the subscales were not affected by the level of income (P-value = 0.644, P-value = 0.170, respectively). Conclusions: Patients with fibromyalgia report a considerable significant impact on their quality of life and impaired sleep quality. Assessment of sleep quality and QoL are needed in patients with fibromyalgia.
Výslovnost ve výuce německého jazyka na ZŠ
Authors: Čeřovská Martina
Published: January 2022

This publication presents an empirical research of pronunciation mistakes of Czech students learning German as a second foreign language at elementary school. The aim of the research was to verify the extent of the students’ pronunciation difficulties with German pronunciation phenomena error-prone for Czech native speakers. The research determined in what way these errors manifest themselves in the transfer from the students’ previously acquired languages, i.e. their mother tongue (Czech) and the first foreign language learnt (English). Within the theoretical foundation, the focus is put on different concepts of pronunciation in terms of foreign language teaching objectives, definitions of language errors and their causes, the development of the German pronunciation standard and methodological approaches to error analysis.
Cofola International 2021
Authors: Bečvářová Bára , Shcherbyna Yevhen , Gyurász Zoltán , Gornaľová Dominika , Wasylkowska-Michór Magdalena , Nový Zdeněk , Plšek Michal , Ševčíková Tereza , Hron Igor , Zhang Kaiqiang , Grodl Lukáš , Malachta Radovan , Zabloudilová Kateřina , Provazník Patrik , Bugyiová Vanessa , Garajová Michaela , Filipová Martina , Barková Veronika , Rakovský Peter , Perkušić Trpimir , Chan Monica
Published: January 2022

Apart from international arbitration, where common state approaches may be seen, e.g. in the field of recognition and enforcement of arbitral awards, some contributions focus on national arbitration, which can bring a comparative point of view of arbitration in different states. Several contributions relate to investment arbitration, in particular, following the Achmea decision. From the most up-to-date topics, some papers are devoted to the COVID-19 pandemic or blockchain technology and their impact on arbitration. Also, the so-called Macao arbitration framework statutory established in 2020 by China will be presented. Traditional topics such as determining the applicability of non-state body of law or rules of law in arbitration or the institute of public policy exception are also included. Arbitration and the issue of double taxation may also bring attention and be beneficial to conference diversity.respective obligations are discussed.
International Investment Court System: The Future of Investment Dispute Settlement?
Authors: Plšek Michal
Published: January 2022

The paper is concerned with examining the texts of the Investment Protection Agreements concluded by the European Union, for the purpose of addressing some of the issues created by the shortcomings of the texts. Focus is given to the provisions related to the Investment Court System established under the respective agreements. The relevant provisions are subject to analysis and conclusions are drawn in an effort to address the issues that arisen. Taken into consideration are works of various other scho-lars who contributed to the topic.
Difference-Making Causation
Authors: Andreas Holger , Günther Mario ,   Journal of Philosophy Inc.
Published: January 2022

We put forth an analysis of causation. The analysis centers on the notion of a causal model that provides only partial information as to which events occur, but complete information about the dependences between the events. The basic idea is this: an event causes another just in case there is a causal model that is uninformative on both events and in which the first event makes a difference as to the occurrence of the other. We show that our analysis captures more causal scenarios than the other counterfactual accounts to date.
Mise en forme et contrôle qualité de données, l’informatique au service de l’écologie
Authors: HUGON Florèn , NAVARRO Xavier , RODRIGUEZ Matt , NEVES Gabriel DAS , BERRONEAU Matthieu , D'AMICO Frank
Published: January 2022

Dans de nombreuses disciplines scientifiques, des études expérimentales ou des suivis sur le terrain assurent la récolte de données. Celles-ci sont stockées sur des fichiers bruts avec un format intuitif, permettant une saisie facile par l’expérimentateur. Cependant, ce format brut est rarement directement compatible avec l’analyse des données récoltées et peut engendrer des analyses erronées ; il est nécessaire d’effectuer une mise en forme et un contrôle qualité des données. Face au nombre de jeux de données brutes croissants et toujours plus massifs, la discipline du numérique pour les sciences du vivant s’est développée. La programmation informatique représente une aide précieuse pour les modélisateurs puisqu’elle permet d’automatiser la mise en forme et le contrôle qualité qui nécessitent souvent un nettoyage des données. Dans cet article, nous présentons une collaboration entre informaticien et modélisateur dans le cadre du suivi de l’abondance d’espèces animales. Les données récoltées sur plusieurs feuilles d’un tableur sont regroupées sur une seule et leur qualité est vérifiée. Les diverses fonctionnalités du programme effectuant cette vérification ont été mises en place à l’aide de la méthode « agile », méthode de développement informatique constituée de sprints. Après la fourniture d’une version du programme, un nouveau sprint définit une nouvelle fonctionnalité à mettre en place par l’informaticien dans une nouvelle version du programme. La première version permet l’appropriation du jeu de données par l’informaticien grâce à la fonctionnalité de mise en forme. Une version plus avancée gère l’absence de données, puis d’autres contrôlent la qualité des données récoltées et rapportent le traitement des anomalies détectées – donnée absente ou erronée ou en dehors d’une plage spécifiée – dans un fichier texte. Ce programme informatique a été explicité afin qu’il puisse être ré-approprié et ré-utilisé. Sa version complète est déposée dans GitHub. Le lien est donné en conclusion.
Genotype-phenotype correlations and long-term efficacy of pamidronate therapy in patients with osteogenesis imperfecta
Authors: Choi Yunha , Hwang Soojin , Kim Gu-Hwan , Lee Beom Hee , Yoo Han-Wook , Choi Jin-Ho
Published: January 2022

Purpose: Osteogenesis imperfecta (OI) is a rare bone fragility disorder caused by defects in type 1 collagen biosynthesis. This study investigated the genotype-phenotype correlations and the efficacy of pamidronate therapy in patients with OI in a single academic center.Methods: This study included 24 patients with OI. A clinical scoring system was used to evaluate disorder severity. COL1A1 and COL1A2 genes were analyzed in 13 patients using Sanger sequencing. Genotype-phenotype correlations and the efficacy of pamidronate therapy were analyzed through a retrospective medical chart review.Results: Of the 24 patients, 18 (75%) were classified as type I (12 with type Ia and 6 with type Ib), 2 as type III (8.4%), and 4 as type IV (16.7%). Type Ia patients showed relatively higher lumbar bone mineral density (BMD) standard deviation scores (SDS) and lower clinical scores than those with other types. Seven patients with qualitative mutations had lower lumbar BMD-SDS (P=0.015) and higher clinical scores (P=0.008) than 6 patients with quantitative mutations. The annual fracture frequency and lumbar BMD-SDS improved in patients with qualitative mutations after pamidronate treatment.Conclusion: This study demonstrated that OI patients with qualitative mutations in COL1A1/2 had a more severe phenotype than those with quantitative mutations. Patients with qualitative mutations showed a significant reduction in fracture frequency and an increase in lumbar BMD-SDS after pamidronate treatment. Clinical score and genotype might be helpful for predicting phenotype and response to pamidronate therapy in OI patients.
Baserer de såkaldte Bartholinannaler sig på danske middelalderlige årbøger?
Authors: Clausen Svend
Published: January 2022

Svend Clausen: Are the Annales Bartholiniani based on Danish medievalannals?

The article discusses the so-called Annales Bartholiniani, written by the Danish scholarThomas Bartholin (1659‑1690). Analysis of a series of events that are clearly dated incorrectlyin the Annales Bartholiniani and a comparison of the dating of these eventswith the content of Danish medieval annals suggest that the inaccurate dating of suchevents in the Annales Bartholiniani must go back to the Danish medieval annalistictraditions. This proves that a great deal of the content of the Annales Bartholinianimust go back either directly or indirectly to the content of Danish medieval annals. Italso points to the fact that there needs to be more discussion of the Annales Bartholinianiin future studies, as they also contain a great deal of information not availableelsewhere, and the close link between them and the content of Danish medieval annalsmakes it much more likely that Bartholin may have used also some Danish medievalannals now lost in addition to the medieval annals still known to us. In this respect,such an identified close link between the Annales Bartholiniani and the known contentof the Danish medieval annalistic traditions may help significantly in explaining alsosome of the otherwise unknown information present in the Annales Bartholiniani.Discussions of the content for years such as 1087 and 1142, for example, suggest thatspecific details such as these may go back directly or indirectly to a now lost medievalDanish annalistic tradition.
Små bøger med vidtfavnende forgreninger - erhvervelser til Judaistisk Samling
Authors: Jansson Eva-Maria
Published: January 2022

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A antecipação de efeitos restritivos à propriedade de não indígenas na pendência de conclusão o processo de demarcação de terras indígenas
Authors: Niebuhr Pedro
Published: January 2022

Oartigo trata da natureza e dos efeitos jurídicos do processo administrativo de demarcação de terras indígenas. O problema investigado consiste em saber se é juridicamente viável impingir restrições aos direitos de não indígenas eventualmente estabelecidos em áreas reivindicadas como de ocupação tradicional por indígenas enquanto pendente de conclusão o processo de demarcação de terras indígenas. São indicados, basicamente, dois argumentos que subsidiariam esse tipo de providência, a saber, (i) o fato de os indígenas possuírem direitos originários sobre as terras por si ocupadas e que, em função disso, (ii) a demarcação de terras indígenas é providência meramente declaratória. Demonstra-se, na presente análise, o conteúdo material e a finalidade do processo demarcatório, bem como se esclarece o fato de que o direito originário às terras tradicionalmente ocupadas pelos indígenas não dispensa a comprovação do preenchimento dos requisitos legais para o reconhecimento deste direito. Conclui-se ser antijurídica a precipitação, a abreviação ou a dispensa do processo demarcatório para efeito de se impor medidas restritivas aos direitos de não indígenas, especialmente se pendente de conclusão o processo demarcatório. O método empregado foi dedutivo, a partir de pesquisa bibliográfica.
Embargo e suspensão ambiental: critérios existentes para aplicação
Authors: Fonseca Wagner Cleyton , Ruschel Caroline
Published: January 2022

Os critérios adotados para medidas administrativas em matéria ambiental são vagos e pouco explorados. Este artigo buscou critérios para aplicação de embargos/suspensões, contribuindo para reduzir a subjetividade dos órgãos ambientais, além de reduzir o controle judicial. Utilizou-se o método dedutivo com pesquisa bibliográfica e documental conforme as seguintes etapas: a) Diferenciação de sanção e medida administrativa de embargo/suspensão; b) Revisão bibliográfica; c) Levantamento da legislação federal e estadual; d) Avaliação dos critérios existentes. Na literatura, há corrente favorável ao embargo/suspensão apenas pela falta de Licença Ambiental, e outra defende que tais medidas se aplicam em reincidências. Na legislação federal, o embargo aplica-se a condutas expressamente vedadas na legislação, e nos estados a falta de Licença justifica o embargo. Quanto à suspensão, exige-se o risco/perigo ao meio ambiente. Os critérios existentes para embargo/suspensão são difíceis de serem caracterizados pelos fiscais, demandando estudos mais aprofundados sobre as práticas dos órgãos ambientais.
O conflito entre o direito à água e o direito de greve: análise da operação carro-pipa do exército brasileiro
Authors: Santos Ivanna Pequeno dos , Tassigny Monica Mota , Filho Antonio Luis Dos Santos
Published: January 2022

O presente estudo tem por propósito analisar a melhor solução para o choque entre direitos normativos no contexto da Operação Carro-Pipa. Destarte, com base em uma investigação documental-bibliográfica na doutrina, jurisprudência e legislação nacional e internacional, exterioriza-se de um lado o direito à água da população excluída que vive em situação de emergência devido à catástrofe da seca no semiárido brasileiro e, de outro lado, o direito de greve dos prestadores de serviço contratados pelo Exército Brasileiro para cumprir a atividade fim – distribuir água potável. Nesse sentido, indaga-se sobre qual critério objetivo promove racionalidade à precedência entre os direitos fundamentais conflitantes no caso concreto desta Operação. Entende-se por intermédio do estudo de caso, a luz da Teoria dos Direitos Fundamentais de Robert Alexy, que o direito de greve, no caso concreto, tem cláusulas de exceção que lhe obriga a ceder ao antagônico direito à água.
O caso Embraer como uma antecipação efetiva da Lei nº 10.973/2004
Authors: Coimbra Elisa Mara , Feres Marcos Vinício Chein
Published: January 2022

Este artigo parte da premissa de que o caso Embraer representa uma antecipação efetiva dos mecanismos legais de incentivo à inovação presentes na Lei nº 10.973/2004. O objetivo é compreender por um raciocínio indutivo como a Embraer se tornou uma referência em inovação sem que existisse um aparato legal estruturado neste sentido, encontrando caminhos muito semelhantes aos hoje institucionalizados. Assim, a partir dos dados encontrados no Decreto-Lei nº 770/1969, procede-se a uma análise do documento, levando em consideração os contextos econômico, político e social, bem como a sua lógica interna. Desse modo, por meio de uma análise de conteúdo, explicitam-se os elementos que foram relevantes para elaboração do Decreto-Lei nº 770/1969 para, posteriormente, indicar quais os institutos foram efetivamente antecipados, ainda antes da Lei nº 10.973/2004. Enfim, a principal contribuição é que estudos futuros possam propor aperfeiçoamentos para os institutos da Lei nº 10.973/2004, a partir da experiência da Embraer.
Os limites da função de poder de polícia do licenciamento ambiental como instrumento de controle para uma gestão integrada dos recursos minerais marinhos
Authors: Araújo Lima Raquel , Costa de Oliveira Carina
Published: January 2022

O artigo tem o objetivo de analisar os limites da função de poder de polícia do licenciamento ambiental para a promoção da gestão integrada dos recursos minerais marinhos, uma vez que o licenciamento ambiental se apresenta como um instrumento de gestão. A metodologia empregada no estudo foi o mapeamento regional, institucional e normativo e elegeu-se o espaço marinho brasileiro como área de estudo. A pesquisa foi realizada no âmbito federal e executada em duas etapas: a primeira referente aos procedimentos de licenciamento encontrados no âmbito do IBAMA e a segunda consistiu na análise dos processos de autorização e concessão de lavra no âmbito da ANM. Ao todo foram selecionados sete processos de licenciamento ambiental. A partir da análise desses procedimentos, observou-se que o licenciamento ambiental pode realizar uma gestão integrada quando alcança uma integração normativa e do quadro institucional. Todavia, alguns limites no âmbito da função de poder de polícia do licenciamento ambiental foram encontrados ao longo do caminho, especialmente quando há falta de controle fiscalizatório das licenças ambientais nas atividades de mineração pelo órgão mineraria e a falta de controle de fiscalização no cumprimento das condicionantes ambientais no regime de outorga mineraria pelo órgão ambiental.
Preexisting memory CD4 T cells in naïve individuals confer robust immunity upon hepatitis B vaccination
Authors: Elias George , Meysman Pieter , Bartholomeus Esther , De Neuter Nicolas , Keersmaekers Nina , Suls Arvid , Jansens Hilde , Souquette Aisha , De Reu Hans , Emonds Marie-Paule , Smits Evelien , Lion Eva , Thomas Paul G , Mortier Geert , Van Damme Pierre , Beutels Philippe , Laukens Kris , Van Tendeloo Viggo , Ogunjimi Benson
Published: January 2022

Antigen recognition through the T cell receptor (TCR) αβ heterodimer is one of the primary determinants of the adaptive immune response. Vaccines activate naïve T cells with high specificity to expand and differentiate into memory T cells. However, antigen-specific memory CD4 T cells exist in unexposed antigen-naïve hosts. In this study, we use high-throughput sequencing of memory CD4 TCRβ repertoire and machine learning to show that individuals with preexisting vaccine-reactive memory CD4 T cell clonotypes elicited earlier and higher antibody titers and mounted a more robust CD4 T cell response to hepatitis B vaccine. In addition, integration of TCRβ sequence patterns into a hepatitis B epitope-specific annotation model can predict which individuals will have an early and more vigorous vaccine-elicited immunity. Thus, the presence of preexisting memory T cell clonotypes has a significant impact on immunity and can be used to predict immune responses to vaccination.
Urinary metabolic biomarkers of diet quality in European children are associated with metabolic health
Authors: Stratakis Nikos , Siskos Alexandros P , Papadopoulou Eleni , Nguyen Anh N , Zhao Yinqi , Margetaki Katerina , Lau Chung-Ho E , Coen Muireann , Maitre Lea , Fernández-Barrés Silvia , Agier Lydiane , Andrusaityte Sandra , Basagaña Xavier , Brantsaeter Anne Lise , Casas Maribel , Fossati Serena , Grazuleviciene Regina , Heude Barbara , McEachan Rosemary RC , Meltzer Helle Margrete , Millett Christopher , Rauber Fernanda , Robinson Oliver , Roumeliotaki Theano , Borras Eva , Sabidó Eduard , Urquiza Jose , Vafeiadi Marina , Vineis Paolo , Voortman Trudy , Wright John , Conti David V , Vrijheid Martine , Keun Hector C , Chatzi Leda
Published: January 2022

Urinary metabolic profiling is a promising powerful tool to reflect dietary intake and can help understand metabolic alterations in response to diet quality. Here, we used 1H NMR spectroscopy in a multicountry study in European children (1147 children from 6 different cohorts) and identified a common panel of 4 urinary metabolites (hippurate, N-methylnicotinic acid, urea, and sucrose) that was predictive of Mediterranean diet adherence (KIDMED) and ultra-processed food consumption and also had higher capacity in discriminating children’s diet quality than that of established sociodemographic determinants. Further, we showed that the identified metabolite panel also reflected the associations of these diet quality indicators with C-peptide, a stable and accurate marker of insulin resistance and future risk of metabolic disease. This methodology enables objective assessment of dietary patterns in European child populations, complementary to traditional questionary methods, and can be used in future studies to evaluate diet quality. Moreover, this knowledge can provide mechanistic evidence of common biological pathways that characterize healthy and unhealthy dietary patterns, and diet-related molecular alterations that could associate to metabolic disease.
A low-tech, cost-effective and efficient method for safeguarding genetic diversity by direct cryopreservation of poultry embryonic reproductive cells
Authors: Hu Tuanjun , Taylor Lorna , Sherman Adrian , Keambou Tiambo Christian , Kemp Steven J , Whitelaw Bruce , Hawken Rachel J , Djikeng Appolinaire , McGrew Michael J
Published: January 2022

Chickens are an important resource for smallholder farmers who raise locally adapted, genetically distinct breeds for eggs and meat. The development of efficient reproductive technologies to conserve and regenerate chicken breeds safeguards existing biodiversity and secures poultry genetic resources for climate resilience, biosecurity, and future food production. The majority of the over 1600 breeds of chicken are raised in low and lower to middle income countries under resource-limited, small-scale production systems, which necessitates a low-tech, cost-effective means of conserving diversity is needed. Here, we validate a simple biobanking technique using cryopreserved embryonic chicken gonads. The gonads are quickly isolated, visually sexed, pooled by sex, and cryopreserved. Subsequently, the stored material is thawed and dissociated before injection into sterile host chicken embryos. By using pooled GFP and RFP-labelled donor gonadal cells and Sire Dam Surrogate mating, we demonstrate that chicks deriving entirely from male and female donor germ cells are hatched. This technology will enable ongoing efforts to conserve chicken genetic diversity for both commercial and smallholder farmers, and to preserve existing genetic resources at poultry research facilities.
Structure of the human ATM kinase and mechanism of Nbs1 binding
Authors: Warren Christopher , Pavletich Nikola P
Published: January 2022

DNA double-strand breaks (DSBs) can lead to mutations, chromosomal rearrangements, genome instability, and cancer. Central to the sensing of DSBs is the ATM (Ataxia-telangiectasia mutated) kinase, which belongs to the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family. In response to DSBs, ATM is activated by the MRN (Mre11-Rad50-Nbs1) protein complex through a poorly understood process that also requires double-stranded DNA. Previous studies indicate that the FxF/Y motif of Nbs1 directly binds to ATM, and is required to retain active ATM at sites of DNA damage. Here, we report the 2.5 Å resolution cryo-EM structures of human ATM and its complex with the Nbs1 FxF/Y motif. In keeping with previous structures of ATM and its yeast homolog Tel1, the dimeric human ATM kinase adopts a symmetric, butterfly-shaped structure. The conformation of the ATM kinase domain is most similar to the inactive states of other PIKKs, suggesting that activation may involve an analogous realigning of the N and C lobes along with relieving the blockage of the substrate-binding site. We also show that the Nbs1 FxF/Y motif binds to a conserved hydrophobic cleft within the Spiral domain of ATM, suggesting an allosteric mechanism of activation. We evaluate the importance of these structural findings with mutagenesis and biochemical assays.
Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodies
Authors: Xu Jun , Kim Ah-Ram , Cheloha Ross W , Fischer Fabian A , Li Joshua Shing Shun , Feng Yuan , Stoneburner Emily , Binari Richard , Mohr Stephanie E , Zirin Jonathan , Ploegh Hidde L , Perrimon Norbert
Published: January 2022

Expansion of the available repertoire of reagents for visualization and manipulation of proteins will help understand their function. Short epitope tags linked to proteins of interest and recognized by existing binders such as nanobodies facilitate protein studies by obviating the need to isolate new antibodies directed against them. Nanobodies have several advantages over conventional antibodies, as they can be expressed and used as tools for visualization and manipulation of proteins in vivo. Here, we characterize two short (<15aa) NanoTag epitopes, 127D01 and VHH05, and their corresponding high-affinity nanobodies. We demonstrate their use in Drosophila for in vivo protein detection and re-localization, direct and indirect immunofluorescence, immunoblotting, and immunoprecipitation. We further show that CRISPR-mediated gene targeting provides a straightforward approach to tagging endogenous proteins with the NanoTags. Single copies of the NanoTags, regardless of their location, suffice for detection. This versatile and validated toolbox of tags and nanobodies will serve as a resource for a wide array of applications, including functional studies in Drosophila and beyond.
Identification and validation of a novel signature for prediction the prognosis and immunotherapy benefit in bladder cancer
Authors: Zhang Yichi , Lin Yifeng , Lv Daojun , Wu Xiangkun , Li Wenjie , Wang Xueqing , Jiang Dongmei
Published: January 2022

Background

Bladder cancer (BC) is a common urinary tract system tumor with high recurrence rate and different populations show distinct response to immunotherapy. Novel biomarkers that can accurately predict prognosis and therapeutic responses are urgently needed. Here, we aim to identify a novel prognostic and therapeutic responses immune-related gene signature of BC through a comprehensive bioinformatics analysis.

Methods

The robust rank aggregation was conducted to integrate differently expressed genes (DEGs) in datasets of the Cancer Genome Atlas (TCGA) and the gene expression omnibus (GEO). Lasso and Cox regression analyses were performed to formulate a novel mRNA signature that could predict prognosis of BC patients. Subsequently, the prognostic value and predictive value of the signature was validated with two independent cohorts GSE13507 and IMvigor210. Finally, quantitative Real-time PCR (qRT-PCR) analysis was conducted to determine the expression of mRNAs in BC cell lines (UM-UC-3, EJ-1, SW780 and T24).

Results

We built a signature comprised the eight mRNAs: CNKSR1, COPZ2, CXorf57, FASN, PCOLCE2, RGS1, SPINT1 and TPST1. Our prognostic signature could be used to stratify BC population into two risk groups with distinct immune profile and responsiveness to immunotherapy. The results of qRT-PCR demonstrated that the eight mRNAs exhibited different expression levels in BC cell lines.

Conclusion

Our study constructed a convenient and reliable 8-mRNA gene signature, which might provide prognostic prediction and aid treatment decision making of BC patients in clinical practice.
Everyday Life under Communism and After: Lifestyle and Consumption in Hungary, 1945–2000
Authors: Valuch Tibor
Published: January 2022

By providing a survey of consumption and lifestyle in Hungary during the second half of the twentieth century, this book shows how common people lived during and after tumultuous regime changes. After an introduction covering the late 1930s, the study centers on the communist era, and goes on to describe changes in the post-communist period with its legacy of state socialism. Tibor Valuch poses a series of questions. Who could be called rich or poor and how did they live in the various periods? How did living, furnishings, clothing, income, and consumption mirror the structure of the society and its transformations? How could people accommodate their lifestyles to the political and social system? How specific to the regime was consumption after the communist takeover, and how did consumption habits change after the demise of state socialism? The answers, based on micro-histories, statistical data, population censuses and surveys help to understand the complexities of daily life, not only in Hungary, but also in other communist regimes in east-central Europe, with insights on their antecedents and afterlives.
Lipoedema and Overweight Leading to Generalised Oedema: A Case Report
Authors: Godoy Jose Maria Pereira De , Godoy LÃ­via Maria Pereira De
Published: January 2022

The aim of the case report was to discuss generalised oedema in a patient with lipoedema and obesity, describing a novel concept of a stage 0 lymphoedema that was denominated as subclinical systemic lymphoedema. A 35-year-old female patient reported to the clinic due to telangiectasia of the lower limbs and leg pain that increased in the heat and when she spend a lot of time in a standing position. The patient had a physical appearance of lipoedema involving the upper and lower limbs associated with a family history of lipoedema, Body Mass Index (BMI) of 33.9 kg/m2. Bioelectrical impedance analysis demonstrated important changes in intracellular and extracellular water as well as in all limbs and the thorax beyond the limits of normality. The telangiectasia was treated with sclerotherapy and pateint was instructed to lose weight and perform physical activity.
Determination of Sexual Dimorphism by Odontometric Study of Permanent Maxillary Incisors and Canines: A Cross-sectional Study from Population of Haryana
Authors: Devi Anju , Gupta Shruti , Kamboj Mala , Narwal Anjali , Kumar Vinay , Raman Rahul
Published: January 2022

Introduction: Sex determination is one of the prime factors employed to assist with the identification of an individual. As extensively mutilated bodies are difficult to identify, teeth can be used as they can withstand bacterial decomposition and fire. Odontometric parameters can be used for gender determination in a specific population. Variations have been documented in different regions in determining sex of an individual using tooth size and accuracy of odontometric sex prediction which prompted us to draw population specific data. Aim: To evaluate permanent maxillary incisors and canines for sexual dimorphism and to estimate the level of accuracy with which they could be used for sex determination in the age group of 18-24 years in Haryana. Materials and Methods: This cross-sectional study comprised of 300 subjects (150 males and150 females) in the age group of 18-24 years, from population of Haryana during January 2017 to May 2017. Maximum mesiodistal (MD) diameter of permanent maxillary incisors and canines were measured using digital vernier calliper. The mean, range, and standard deviation were calculated for the size of the teeth. A two-sample t-test was used to test for statistical difference between means. Results: The present study comprised of 300 individuals (150 males with mean age of 20.81 years and 150 females with mean age 20.54 years) between the age group of 18-24 years. Mean mesiodistal dimension for each tooth was found to be greater in males as compared to females with statistically significant difference for right canine (p-value=0.003), left canine (p-value=0.001) and right lateral incisor (p-value=0.019). Left maxillary canine showed highest percentage (3.164%) of sexual dimorphism. The level of accuracy of sex determination when all the teeth under study were taken into consideration was 62.7% of females and 58% of males. Conclusion: Sexual dimorphism of teeth is population specific and was well evident in population of Haryana, hence the mesiodistal dimensions in maxillary left permanent canine can aid in sex determination.
Nail Patterns in Papulosquamous Disorders- An Observational Study from Southern India
Authors: Antony Amutha , Sivaprakasam Karthika , Mathan Revathy
Published: January 2022

Introduction: Nail changes are always a cause of physical handicap and psychological stress in this era where utmost importance is given to cosmetic appearance. Other than trauma and infections, Papulosquamous disorders are major contributor to nail changes. Thorough knowledge of their patterns will aid in early detection and treatment before irreversible damage to nails occurs. Aim: To observe the clinical patterns of nail changes in various papulosquamous disorders. Materials and Methods: This observational descriptive cross- sectional study was conducted in a tertiary care set up at Government Coimbatore Medical College, Coimbatore from June 2016 to June 2017. The study group comprised of 104 cases of various papulosquamous disorders. The type of papulosquamous disorder, presence of nail changes, their onset, duration, various patterns and associated co-morbidities were noted in detail. The observed data were entered in Microsoft Excel and data analysis was done in Epi Info system. Results: Out of the 104 cases taken for study 71 (68%) had nail changes. Majority of them were in 31-45 years age group (n=29; 41%). More of males (n=38, 54%) had nail changes than the females (n=33, 46%). Psoriasis (n=47; 66%) was the most common papulosquamous disorder with nail changes followed by lichen planus (n=9; 13%) and pityriasis rubrapilaris (n=7; 10%). Pitting of nails was the most common nail pattern (n=44; 62%), followed by subungual hyperkeratosis (n=24; 34%), thickened nail plate and longitudinal striations (both n=19; 27%). Finger nails were more affected than toe nails. Psoriasis and pityriasis rubrapilaris had 100% nail involvement. The patterns of nail changes were maximum in psoriasis and lichen planus. Beaus lines was observed in most of the papulosquamous disorders except Lichen nitidus and Lichen striatus. The time gap for onset of nail changes was longer for psoriasis (4.2 years) and shorter for pityriasis rosea (two months). In lichen striatus and lichen nitidus, nails were involved only in children. Minimal nail changes were observed in para psoriasis and pityriasis lichenoides. More number of co-morbidities like diabetes, hypertension, atopy and arthritis were seen in psoriasis. Conclusion: In the present study, it was observed that the nail changes were more common in psoriasis, lichen planus and pityriasis rubra pilaris among the papulosquamous disorders. The highly frequent nail patterns were pitting and subungual hyperkeratosis.
Effect of Squat Training with Whole Body Vibration on Balance and Functional Performance in Elderly- A Quasi-experimental Study
Authors: Rathi Manisha Ashish , Joshi Reema , Desai Roopa , Gazbare Preeti , Kulkarni Neha , Balid Maryson
Published: January 2022

Introduction: Elderly population shows many health related disorders which disrupt their functional performance. Exercise training can alter muscle strength, balance, gait, endurance and finally physical function. Whole Body Vibration (WBV) may enhance reduced performance. Hence, the present study evaluated the effects WBV on balance and functional performance in elders. Aim: To assess and compare the effects of squat training on WBV versus plane surface on balance using Timed Get-Up and Go Test (TUG) and functional performance using Short Physical Performance Battery (SPPB). Materials and Methods: This quasi-experimental study was conducted at Dr. D.Y. Patil College of Physiotherapy, Pune, Maharashtra, India from August 2015 to March 2016, including 30 healthy elderly individuals with age between 60 to 70 years. They were divided into group A (Squat training on vibratory surface) and group-B (squat training on plane surface). Training was given for four weeks, three sessions per week. Pretest and post-test values of TUG and SPPB which included balance, gait speed and lower extremity strength was assessed. Epi Info version 7 software was used for statistical analysis. Results: Mean age in group A was 64.53±3.22 years and group B was 65±3.81 years. Group A showed significant reduction (p-value <0.001) in time taken to complete TUG from 15.27 seconds to 12.53 seconds and also group B showed improvement from 14.53 seconds to 13.93 seconds. The SPPB score showed improvement in balance (p-value <0.001), gait speed (p-value <0.001) and strength (p-value <0.001) in group A and gait speed (p-value <0.001) in group B. When compared, Group A showed significant improvement in TUG (p-value=0.01) and lower extremity performance (p-value <0.001) than group B. Conclusion: Squat training on vibratory surface significantly improves balance and functional performance in elderly. Hence, this training can be included as a routine exercise for elderly population with all precautions.
Malignant Transformation of Oral Lichen Planus: A Peril of COVID-19 Pandemic
Authors: Deb Debarati , Sinha Rupam , Maity Subhadeep , Jha Harsh Vardhan , Datta Mainak
Published: January 2022

Oral Lichen Planus (OLP) is a chronic autoimmune inflammatory disease of the oral mucosa. The erosive variety of OLP is multifocal, ulcerative, and painful in nature. Patients with OLP have a higher risk of developing Oral Squamous Cell Carcinomas (OSCCs). Here, authors presents a case of 49-year-old female patient presented to the Department of Oral Medicine and Radiology with the chief complaint of burning sensation in the inner aspect of right cheek, since one year. A diffuse whitish area surrounded by erythematous area was present in the right buccal mucosa. Lesion was diagnosed primarily as erosive Lichen Planus (LP). She discontinued the medication and did not come for follow-up visits because of the Coronavirus Disease-2019 (COVID-19) pandemic. After 10 months, she came back with pain and a diffuse swelling with indurated margin in the same region involving right buccal vestibule and retromolar area. After incisional biopsy it was found that the lesion has progressed to squamous cell carcinoma, and the patient was referred to the Department of Oral Surgery where segmental mandibulectomy was done with placement of free fibula graft. The OLP is considered as a potentially malignant disorder and patient should be advised to avoid additional risk factors such as tobacco and alcohol and should be followed at minimum one year interval as prognosis and patient survival is directly related to stage and grade of cancer at its initial diagnosis.
Contribution of the Characteristics of Farmers to use of Coping Strategies towards Household Food Security
Authors: Haque M. E. , Islam M. N. , Mostafa M.G. , Majid M. A. , Islam M. R. , Haque M. M. , Uddin M. Y. , Alam M. J. , Tasnim J. , Muhammad N. , Turin M. Z.
Published: January 2022

A study was carried out at each of three flood affected reverine villages of three upazilas (small administrative unit) under Jamalpur district in Bangladesh during September, 2011 to May, 2012 to find out the contribution of the characteristics of the farmers to the use of coping strategies towards household food security practiced by the farmers during flood. Data were collected from randomly selected 336 respondents both the qualitative and quantitative techniques and analyzed with the help of SPSS. Out of 18 characteristics, 11 had positive, 2 had negative and 5 had no significant relation with coping strategies towards household food security during flood period. Stepwise regression analysis revealed that six variables namely participation in income generating activities (IGAs) (20.1 percent), knowledge on flood coping mechanisms (8.5 percent), cosmopoliteness (6.5 percent), utilization percentage of received credit (2.0 percent), water and sanitation condition  (1.6 percent) and year round household food situation (0.8 percent) were the major contributing variables which combindly explained 39.5 percent of total variations. Path analysis revealed that knowledge on flood coping mechanisms had the highest positive direct effects (0.285) and participation in IGAs had highest positive indirect effects (0.169) on coping strategy practices. Considering the relative contribution on the coping strategy practices towards household food security during flood period, based on their direct effects, the six variables could be arranged as follows knowledge on flood coping mechanism > participation in income generating activities > cosmopoliteness > water and sanitation condition > year round household food situation > utilization of received credit.
Effect of Packaging Types on Quality of Red Tilapia Shredded Added by Cayenne Pineapple (Ananas comosus (L) Merr) Fruit Solution
Authors: Najmia Muflihatun , Rostini Iis , Gumilar Iwang , Pratama Rusky Intan
Published: January 2022

The purpose of this research is to determine the right type of packaging for organoleptic quality and analysis of the peroxide value of red tilapia. This research uses an experimental method consisting of 3 types of packaging, namely (Aluminum Foil), (Plastic High Density Polyethylene (HDPE), Plastic Low Density Polyethylene (LDPE) using 20 panelists as replication. The parameters observed in this research are organoleptic characteristics (appearance, texture, aroma, and taste) based on the panelists preference level using hedonic test, chemical analysis (peroxide number test) and storage for 28 days in an incubator with a temperature of 25ºC and 45ºC for each treatment. The chemical test results were analyzed descriptively while the organoleptic test results were analyzed using the Friedman test. Based on the Friedman test, it was found that HDPE plastic packaging with the addition of a solution of pineapple cayenne was the preferred treatment for panelists compared to other treatments. Based on the Bayes test, taste is the most important criterion compared to appearance, texture, and aroma. The results of the peroxide value analysis for each type of packaging treatment during 28 days of storage at a temperature of 25°C showed results with a peroxide value of 0 meq/kg and 45°C showed results with a peroxide value of 0 meq/kg per week.
Digitization in Detecting Fish Freshness Quality: Literature Review
Authors: Pratiwy Fittrie Meyllianawaty , . Aisyah , Mulyani Yuniar
Published: January 2022

Fish contain a lot of nutrients that are very good for the body, but fish are often traded in a postmortem state with poor quality. One of the reasons is the difficulty of detecting the quality of fish freshness. Many studies currently make applications and special tools to see the fish quality in the digitalization of tools to facilitate human work. The method used is by identifying digital images, acoustic techniques, and electronic noses. In digital image identification, the fish’s eye image is applied by taking pictures of the condition of the fish on a smartphone device and then processing it using the small square method or curve fitting. After that, the identification of the acoustic form utilizes sound waves emitted to the observed target. In addition, the electric nose method is carried out by using the nose's working principle, which receives a response to odors that arise from rotting fish.
Impact of Climate Change on Arid Region: Case Study of Rajasthan
Authors: De Moushila , Dhote Meenakshi
Published: January 2022

The climate is changing at a very quick rate adding to the expanded global concern. During the most recent couple of years, the noble Coronavirus held the world by a threat that caused huge financial mishaps and loss of lives. Simultaneously, the extreme weather events kept on adding to the outrageous climate crisis. To mitigate the effect of climate change, the entire world met up and made various commitments made in various UN conventions, culminating in the Paris Convention to both implement mitigation and adaptation measures to reduce the emissions. The Sustainable Development Goals plan was recognized by all individuals from the United Nations in 2012 at Rio De Janeiro with an expect to propel a sound and created future for the planet and its people. It was in 2015 the Sustainable Development Goals (SDGs) were embraced with 17 goals; with SDGs Goal No.13 addressing earnest activities to combat climate change and its impact in all topographical elements including arid areas. The paper is an attempt to give an overview of the Arid region, climate vulnerability, and to identify the main challenges which climate change presents to the region's future development. The paper further explores the impact of climate change in an arid area and the variation and alleviation ways to deal with address the worries of climate change and closes with methods of freeing the impact from climate change in a parched Rajasthan.