bluuebunny/crossref_metadata_2025 huggingface dataset:
Reservoir landslide monitoring and mechanism analysis based on UAV photogrammetry and sub-pixel offset tracking: a case study of Wulipo landslide
Authors: Li Zhi-Hai , Jiang Nan , Shi An-Chi , Zhao Liu-Yuan , Xian Zhao , Luo Xiang-Long , Li Hai-Bo , Zhou Jia-Wen
Published: January 2024

Introduction: Reservoir landslides undergo large deformations during the early stages of impoundment and maintain long-term persistent deformations during the operational period of the reservoir. The management of reservoir landslides mostly focuses on the early identification, risk assessment during the large deformations, and long-sequence monitoring during long-term persistent deformations, which requires sufficient continuity and integrity of the landslide monitoring data.

Methods: Taking the Wulipo (WLP) landslide in Baihetan Reservoir as example, this paper proposes a reservoir landslide monitoring method that integrates field survey, unmanned aerial vehicle (UAV) photogrammetry and global navigation satellite system (GNSS) monitoring, which can effectively eliminate the practical monitoring gaps between multiple monitoring methods and improve the continuity and completeness of monitoring data.

Results and discussion: First, this study determined the initiation time of the landslide through the field investigation and collected five period of UAV data to analyze the overall displacement vector of the WLP landslide using sub-pixel offset tracking (SPOT). On the basis of the above data, we compensated for the missing data in GNSS system due to the practical monitoring vacancies by combining the field survey and the landslide-water level relationship. Based on these monitoring data, this paper points out that the WLP landslide is a buoyancy-driven landslide, and whether or not accelerated deformation will occur is related to the maximum reservoir water level. Finally, this study analyzed and discussed the applicability of UAV photogrammetry for reservoir landslide monitoring in the absence of ground control points (GCPs), and concluded that this method can be quickly and flexibly applied to the stage of large deformation of reservoir landslides.
STEM teachers’ beliefs about the relevance and use of evidence-based information in practice: a case study using thematic analysis
Authors: Hillmayr Delia , Reinhold Frank , Holzberger Doris , Reiss Kristina
Published: January 2024

The term evidence-based practice has gained importance in teacher education as well as in everyday school life. Calls from policymakers, academics, and society have become increasingly apparent that teachers’ professional actions should not exclusively be based on subjective experiential knowledge but also on empirical evidence from research studies. However, the use of evidence comes along with several challenges for teachers such as often lacking applicability of available sources or limited time resources. This case study explores how teachers (n = 12) at secondary schools think about the relevance and usage of evidence-based information in practice as well as the barriers associated with it. As we see a particular need for evidence-based teaching in STEM disciplines, we focus on these subjects. A thematic analysis of the data indicates that the teachers generally rate relevance highly, for instance seeing opportunities for support and guidance. However, the actual use of evidence-based information in the classroom is rather low. The teachers most frequently mentioned the feasibility of implementation in class as a quality indicator of evidence-based information. Based on the data, we discuss possible conclusions to promote evidence-based practice at schools. Furthermore, the study opens up directions for further research studies with representative teacher samples in various disciplines.
Cloning, distribution, and effects of growth regulation of MC3R and MC4R in red crucian carp (Carassius auratus red var.)
Authors: Huang Lu , Deng Xin , Yang Xiangqiong , Tang Zhong , Fan Siyu , Zhou Zhifeng , Tao Min , Liu Shaojun
Published: January 2024

Background

Melanocortin-3 and -4 receptors (MC3R and MC4R), G protein-coupled receptors, play vital roles in the regulation of energy homeostasis. To understand the functions of mc3r and mc4r in the energy homeostasis of red crucian carp (Carassius auratus red var., RCC), we cloned mc3r and mc4r, analyzed the tissue expression and localization of the genes, and investigated the effects of knockout of mc3r (mc3r+/-) and mc4r (mc4r+/-) in RCC.

Results

The full-length cDNAs of RCC mc3r and mc4r were 1459 base pairs (bp) and 1894 bp, respectively. qRT-PCR indicated that mc3r and mc4r were profusely expressed in the brain, but lower expressed in the periphery tissues. ISH revealed that mc3r and mc4r were located in NPP, NPO, NAPv, NSC, NAT, NRL, NLTl, and NLTp of the brain, suggesting that mc3r and mc4r might regulate many physiological and behavioral aspects in RCC. To further verify the roles of mc3r and mc4r in energy homeostasis, the mc3r+/- and mc4r+/- fish were obtained by the CRISPR/Cas9 system. The average body weights, total lengths, body depths, and food intake of mc4r+/- fish were significantly higher than those of mc3r+/- and the normal wild-type (WT) fish, but there was no difference between the mc3r+/- and WT fish, indicating that the RCC phenotype and food intake were mainly influenced by mc4r but not mc3r. Interestingly, mc4r+/- fish displayed more visceral fat mass than mc3r+/- and WT fish, and mc3r+/- fish also exhibited slightly more visceral fat mass compared to WT. RNA-seq of the liver and muscle revealed that a large number of differentially expressed genes (DEGs) differed in WT vs. mc3r+/-, WT vs. mc4r+/-, and mc3r+/- vs. mc4r+/-, mainly related to lipid, glucose, and energy metabolism. The KEGG enrichment analysis revealed that DEGs were mainly enriched in pathways such as steroid biosynthesis, fatty acid metabolism, fatty acid biosynthesis, glycolysis/gluconeogenesis, wnt signaling pathway, PPAR signaling pathway, and MAPK signaling pathway, thereby affecting lipid accumulation and growth.

Conclusion

In conclusion, these results will assist in the further investigation of the molecular mechanisms in which MC3R and MC4R were involved in the regulation of energy homeostasis in fish.
Soil nutrients determine leaf traits and above-ground biomass in the tropical cloud forest of Hainan Island
Authors: Batool Farwa , Bahadur Saraj , Long Wenxing
Published: January 2024

Identifying soil characteristics associated with the plant’s resource use and acquisition strategy at different scales could be a crucial step to understanding community assembly and plant strategy. There is an increasing trend that plant functional properties can be an important driver of ecosystem functioning. However, major knowledge gaps exist about how soil abiotic properties, shape species diversity, above-ground biomass (AGB) and plant functional diversity in the Bawangling tropical forest (TCF) of Hainan island. Hence we hypothesized that plant functional traits and above-ground biomass would be strongly associated with soil abiotic factors given their direct relationship to soil resource acquisition and use. Here, we used 12 plant functional traits (FTs), above-ground biomass (AGB), and five soil nutrients in the Bawangling tropical cloud forest of Hainan Island by using a polynomial regression model and multivariate correlations to show relationship and identify how plants allocate their limited resources to adapt to their surroundings. Various phytosociological attributes were assessed and an Importance Value Index (IVI) value was calculated for each species to determine the dominant species. More than half of the total variations could be attributed to interspecific variations in H, DBH, LA, LMA, and LDW. From a taxonomic perspective; we found that species-level variance was more significant for plant functional traits and soil nutrients like TN, AP, TP, and OM. On the other hand, variation in specific stem density (SSD), leaf thickness (LT), leaf phosphorus (LP) and leaf soluble sugar (LS) was an exception for these tendencies. Among soil nutrients, soil nitrogen and phosphorus significantly impact the species and functional traits. Furthermore, the soil AN and TP we also found to have a comparatively strong positive relationship with above-ground biomass (AGB) as compared with other soil nutrients. The morpho-physiological functional traits showed a trade-off between conservative and acquisitive resource usage. These variations suggested that the relationships of functional traits, AGB, and species with soil nutrients mainly AN and TP in tropical cloud forests can directly affect the growth, reproduction, and survival of the species and are beneficial for the species co-existence and maintenance of biodiversity.
Chronic stress from adolescence to adulthood increases adiposity and anxiety in rats with decreased expression of Krtcap3
Authors: Szalanczy Alexandria M. , Fitzpatrick Mackenzie , Beeson Angela , Bui Trangdai , Dyson Christina , Eller Seth , Landry Julia , Scott Christina , Grzybowski Michael , Klotz Jason , Geurts Aron M. , Weiner Jeff L. , Redei Eva E. , Solberg Woods Leah C.
Published: January 2024

We previously identified Keratinocyte-associated protein 3, Krtcap3, as a novel adiposity gene, but subsequently found that its impact on adiposity may depend on environmental stress. To more thoroughly understand the connection between Krtcap3, adiposity, and stress, we exposed wild-type (WT) and Krtcap3 knock-out (KO) rats to chronic stress then measured adiposity and behavioral outcomes. We found that KO rats displayed lower basal stress than WT rats under control conditions and exhibited metabolic and behavioral responses to chronic stress exposure. Specifically, stress-exposed KO rats gained more weight, consumed more food when socially isolated, and displayed more anxiety-like behaviors relative to control KO rats. Meanwhile, there were minimal differences between control and stressed WT rats. At study conclusion stress-exposed KO rats had increased corticosterone (CORT) relative to control KO rats with no differences between WT rats. In addition, KO rats, independent of prior stress exposure, had an increased CORT response to removal of their cage-mate (psychosocial stress), which was only seen in WT rats when exposed to chronic stress. Finally, we found differences in expression of the glucocorticoid receptor, Nr3c1, in the pituitary and colon between control and stress-exposed KO rats that were not present in WT rats. These data support that Krtcap3 expression affects stress response, potentially via interactions with Nr3c1, with downstream effects on adiposity and behavior. Future work is necessary to more thoroughly understand the role of Krtcap3 in the stress response.
Deciphering the link: ferroptosis and its role in glioma
Authors: Wang He , Liu Yingfeng , Che Shusheng , Li Xiangjun , Tang Dongxue , Lv Shaojing , Zhao Hai
Published: January 2024

Glioma, as the most frequently occurring primary malignancy in the central nervous system, significantly impacts patients’ quality of life and cognitive abilities. Ferroptosis, a newly discovered form of cell death, is characterized by significant iron accumulation and lipid peroxidation. This process is fundamentally dependent on iron. Various factors inducing ferroptosis can either directly or indirectly influence glutathione peroxidase, leading to reduced antioxidant capabilities and an increase in lipid reactive oxygen species (ROS) within cells, culminating in oxidative cell death. Recent research indicates a strong connection between ferroptosis and a range of pathophysiological conditions, including tumors, neurological disorders, ischemia-reperfusion injuries, kidney damage, and hematological diseases. The regulation of ferroptosis to intervene in the progression of these diseases has emerged as a major area of interest in etiological research and therapy. However, the exact functional alterations and molecular mechanisms underlying ferroptosis remain to be extensively studied. The review firstly explores the intricate relationship between ferroptosis and glioma, highlighting how ferroptosis contributes to glioma pathogenesis and how glioma cells may resist this form of cell death. Then, we discuss recent studies that have identified potential ferroptosis inducers and inhibitors, which could serve as novel therapeutic strategies for glioma. We also examine the current challenges in targeting ferroptosis in glioma treatment, including the complexity of its regulation and the need for precise delivery methods. This review aims to provide a comprehensive overview of the current state of research on ferroptosis in glioma, offering insights into future therapeutic strategies and the broader implications of this novel cell death pathway in cancer biology.
Bomb radiocarbon determines absolute age of adult fin whales, and validates use of earplug growth bands for age determination
Authors: Campana Steven E. , Finnsdóttir Sigríður Vala , Sigurðsson Guðjón Már
Published: January 2024

Baleen whales are one of the few vertebrate taxa for which there are no confirmed estimates of longevity or methods of age determination. Lamina counts in the waxy earplug are assumed to represent age, but ageing accuracy is completely unknown. In this study, bomb radiocarbon assays of the earplug growth sequence in three adult fin whales (Balaenoptera physalus) were used to prepare the most complete within-individual bomb radiocarbon chronologies yet reported for any vertebrate. The whale radiocarbon chronologies matched those of known-age carbonate reference chronologies very well, indicating that the earplug laminae were both metabolically stable and formed throughout the life of the whale. Earplug lamina counts accurately represented absolute ages of 65-85 yr to within 6% of the correct age. Detection of a significant declining trend in δ13C with year of lamina formation within individual whales was consistent with that of the Suess effect, again underlining the metabolic stability of the earplug laminae. Given our results, recent applications of earplug laminae for reconstructing diet and life history events appear to be firmly based, with the potential for further elemental and isotopic applications analogous to those of the otolith.
Acute graft versus host disease 1976–2020: reduced incidence and predictive factors
Authors: Di Francesco Alessandra , Raiola Anna Maria , Dominietto Alida , Di Grazia Carmen , Gualandi Francesca , Van Lint Maria Teresa , Bregante Stefania , Chiusolo Patrizia , Laurenti Luca , Sora Federica , Giammarco Sabrina , Metafuni Elisabetta , Fresa Alberto , Sica Simona , Angelucci Emanuele , Bacigalupo Andrea
Published: January 2024

We studied the incidence of acute graft versus host disease (GvHD) and its outcome in three consecutive time frames (year <2000; 2000–2010; >2010), in 3,120 patients allografted in two transplant Centers between 1976 and 2020. The median age increased over the three periods from 32 to 42 to 54 years (p < 0.00001). The median day of onset of GvHD in the three periods was day +14, day +16, and day +30, respectively (p < 0.0001). The cumulative incidence (CI) of GvHD grades II–IV in the three periods was 47, 24, and 16%, respectively (p < 0.00001). The CI of GvHD grades III–IV was 13, 5, and 4% (p < 0.001). In multivariate analysis, significant predictive factors for GvHD II–IV, on top of year of transplant, were anti-thymocyte globulin (ATG) (RR 0.67, p > 0.001); post-transplant cyclophosphamide (PTCY) (RR 0.41, p < 0.001), a family mismatched donor (RR 1.31, p = 0.03) a matched unrelated donor (RR 2.1, p < 0.001), an unrelated mismatched donor (RR1.8, p = 0.001), donor age above 40 years (RR 1.27, p < 0.001), hematological malignancy—as compared to aplastic anemia (RR 2.3, p < 0.001). When selecting only GvHD grade II, in a multivariate analysis, there was a significant reduction of transplant-related mortality (TRM) for patients grafted in 2001–2010 (RR 0.62, p < 0.0001) and for patients grafted in 2011–2020 (RR 0.35, p < 0.0001) as compared to grafts before the year 2000. A similar reduction in time was seen for patients with GvHD grades III–IV. The overall TRM in the three periods was 30, 22, and 16% (p < 0.0001) and survival was 47, 51, and 58% (p < 0.0001). Relapse risk was unchanged. In conclusion, we showed improved prevention of acute GvHD with time, together with a significant delay in the onset of the disease. Treatment of GvHD has also improved over time, as suggested by both reduced TRM and improved survival in more recent transplant periods.
Metabolic-associated fatty liver disease: a selective review of pathogenesis, diagnostic approaches, and therapeutic strategies
Authors: Habibullah Mohammad , Jemmieh Khaleed , Ouda Amr , Haider Mohammad Zulqurnain , Malki Mohammed Imad , Elzouki Abdel-Naser
Published: January 2024

Background

Metabolic associated fatty liver disease (MAFLD) is a novel terminology introduced in 2020 to provide a more accurate description of fatty liver disease associated with metabolic dysfunction. It replaces the outdated term nonalcoholic fatty liver disease (NAFLD) and aims to improve diagnostic criteria and tailored treatment strategies for the disease. NAFLD, the most prevalent liver disease in western industrialized nations, has been steadily increasing in prevalence and is associated with serious complications such as cirrhosis and hepatocellular carcinoma. It is also linked to insulin resistance syndrome and cardiovascular diseases. However, current studies on NAFLD have limitations in meeting necessary histological endpoints.

Objective

This literature review aims to consolidate recent knowledge and discoveries concerning MAFLD, integrating the diverse aspects of the disease. Specifically, it focuses on analyzing the diagnostic criteria for MAFLD, differentiating it from NAFLD and alcoholic fatty liver disease (AFLD), and exploring the epidemiology, clinical manifestations, pathogenesis, and management approaches associated with MAFLD. The review also explores the associations between MAFLD and other conditions. It discusses the heightened mortality risk associated with MAFLD and its link to chronic kidney disease (CKD), showing that MAFLD exhibits enhanced diagnostic accuracy for identifying patients with CKD compared to NAFLD. The association between MAFLD and incident/prevalent CKD is supported by cohort studies and meta-analyses.

Conclusion

This literature review highlights the importance of MAFLD as a distinct terminology for fatty liver disease associated with metabolic dysfunction. The review provides insights into the diagnostic criteria, associations with CKD, and management approaches for MAFLD. Further research is needed to develop more accurate diagnostic tools for advanced fibrosis in MAFLD and to explore the underlying mechanisms linking MAFLD with other conditions. This review serves as a valuable resource for researchers and healthcare professionals seeking a comprehensive understanding of MAFLD.
Metabarcoding of zooplankton communities of Dianchi Lake based on the mitochondrial cytochrome oxidase subunit 1 gene
Authors: Cen Fu , Xu Shan , Yin Genshen , Dong Minghua
Published: January 2024

Freshwater lakes as an essential component of the ecosystem, provide ecological resources in addition to economic source for humans. Under recent climate change scenario, preserving the biodiversity of freshwater ecosystems is crucial. This study aimed to characterize the diversity of zooplankton communities in Dianchi Lake, located in Kunming Municipality, Yunnan Province, China, using Illumina high-throughput sequencing of the cytochrome oxidase subunit 1 (COI) gene marker. A total of 18 water samples were collected including 16 from the outer sea area of Dianchi Lake: 4 from the east (E1-4), 4 from the west (W1-4), 4 from the south (S1-4), and 4 from the north (N1-4), and: 2 from the Caohai area (C1-2) as research sites. All environmental parameters including pH, ammonium (NH4+), total nitrogen (TN), total phosphorus (TP), chlorophyll a content (CHLA) were found to be insignificant (p > 0.05), except for chemical oxygen demand (COD) and transparency (T), which were found to be significant (p < 0.05). Alpha diversity indices including ACE, Chao1, Shannon, and Simpson showed non-significant differences (p > 0.05), indicating no variation in the richness of zooplankton communities at different locations of Dianchi Lake. However, principal coordinate analysis (PCoA) showed that most of the samples from East, West, and South groups were close to each other, showing more similarities among them, while Caohai and North group samples were distant from each other, showing more differences with other groups. Rotifera, Arthropoda, and Chordata were the top three phyla, while Keratella, Macrothrix, and Brachionus were the dominant genera. Mantel test analysis showed that COD and transparency were important environmental factors that shaped the Rotifera community structure of Dianchi Lake. In conclusion, this study provides insights on conserving the diversity of zooplankton communities in Dianchi Lake, especially by controlling COD and maintaining water transparency, in order to preserve its ecological resources and economic significance.
Influenza vaccine compatibility among hospitalized patients during and after the COVID-19 pandemic
Authors: Fratty Ilana S. , Jurkowicz Menucha , Zuckerman Neta , Nemet Ital , Atari Nofar , Kliker Limor , Gur-Arie Lea , Rosenberg Alina , Glatman-Freedman Aharona , Lustig Yaniv , Mandelboim Michal
Published: January 2024

Introduction

Following the significant decrease in SARS-CoV-2 cases worldwide, Israel, as well as other countries, have again been faced with a rise in seasonal influenza. This study compared circulating influenza A and B in hospitalized patients in Israel with the influenza strains in the vaccine following the 2021–2022 winter season which was dominated by the omicron variant.

Methods

Nasopharyngeal samples of 16,325 patients were examined for the detection of influenza A(H1N1)pdm09, influenza A(H1N1)pdm09 and influenza B. Phylogenetic trees of hemagglutinin were then prepared using sanger sequencing. Vaccine immunogenicity was also performed using the hemagglutination inhibition test.

Results

Of the 16,325 nasopharyngeal samples collected from hospitalized patients between September 2021 (Week 40) and April 2023 (Week 15), 7.5% were found to be positive for influenza. Phylogenetic analyses show that in the 2021–2022 winter season, the leading virus subtype was influenza A(H3N2), belonging to clade 3C.2a1b.2a.2. However, the following winter season was dominated by influenza A(H1N1)pdm09, which belongs to clade 6B.aA.5a.2. The circulating influenza A(H1N1)pdm09 strain showed a shift from the vaccine strain, while the co-circulating influenza A(H3N2) and influenza B strains were similar to those of the vaccine. Antigenic analysis coincided with the sequence analysis.

Discussion

Influenza prevalence during 2022–2023 returned to typical levels as seen prior to the emergence of SARS-CoV-2, which may suggest a gradual viral adaptation to SARS-CoV-2 variants. Domination of influenza A(H1N1)pdm09 was observed uniquely in Israel compared to Europe and USA and phylogenetic and antigenic analysis showed lower recognition of the vaccine with the circulating influenza A(H1N1)pdm09 in Israel compared to the vaccine.
Comparative genomic analysis of uropathogenic Escherichia coli strains from women with recurrent urinary tract infection
Authors: Flores-Oropeza Marco A. , Ochoa Sara A. , Cruz-Córdova Ariadnna , Chavez-Tepecano Rolando , Martínez-Peñafiel Eva , Rembao-Bojórquez Daniel , Zavala-Vega Sergio , Hernández-Castro Rigoberto , Flores-Encarnacion Marcos , Arellano-Galindo José , Vélez Daniel , Xicohtencatl-Cortes Juan
Published: January 2024

Introduction

Recurrent urinary tract infections (RUTIs) caused by uropathogenic Escherichia coli are costly public health problems impacting patients’ quality of life.

Aim

In this work, a comparative genomics analysis of three clinical RUTI strains isolated from bladder biopsy specimens was performed.

Materials and methods

One hundred seventy-two whole genomes of urinary tract E. coli strains were selected from the NCBI database. The search for virulence factors, fitness genes, regions of interest, and genetic elements associated with resistance was manually carried out. The phenotypic characterization of antibiotic resistance, haemolysis, motility, and biofilm formation was performed. Moreover, adherence and invasion assays with human bladder HTB-5 cells, and transmission electron microscopy (TEM) were performed.

Results

The UTI-1_774U and UTI-3_455U/ST1193 strains were associated with the extraintestinal pathotypes, and the UTI-2_245U/ST295 strain was associated with the intestinal pathotype, according to a phylogenetic analysis of 172 E. coli urinary strains. The three RUTI strains were of clinical, epidemiological, and zoonotic relevance. Several resistance genes were found within the plasmids of these strains, and a multidrug resistance phenotype was revealed. Other virulence genes associated with CFT073 were not identified in the three RUTI strains (genes for type 1 and P fimbriae, haemolysin hlyA, and sat toxin). Quantitative adherence analysis showed that UTI-1_774U was significantly (p < 0.0001) more adherent to human bladder HTB-5 cells. Quantitative invasion analysis showed that UTI-2_245U was significantly more invasive than the control strains. No haemolysis or biofilm activity was detected in the three RUTI strains. The TEM micrographs showed the presence of short and thin fimbriae only in the UTI-2_245U strain.

Conclusion

The high variability and genetic diversity of the RUTI strains indicate that are a mosaic of virulence, resistance, and fitness genes that could promote recurrence in susceptible patients.
Otitis media: recent advances in otitis media vaccine development and model systems
Authors: Zahid Ayesha , Wilson Jennifer C. , Grice I. Darren , Peak Ian R.
Published: January 2024

Otitis media is an inflammatory disorder of the middle ear caused by airways-associated bacterial or viral infections. It is one of the most common childhood infections as globally more than 80% of children are diagnosed with acute otitis media by 3 years of age and it is a common reason for doctor’s visits, antibiotics prescriptions, and surgery among children. Otitis media is a multifactorial disease with various genetic, immunologic, infectious, and environmental factors predisposing children to develop ear infections. Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are the most common culprits responsible for acute otitis media. Despite the massive global disease burden, the pathogenesis of otitis media is still unclear and requires extensive future research. Antibiotics are the preferred treatment to cure middle ear infections, however, the antimicrobial resistance rate of common middle ear pathogens has increased considerably over the years. At present, pneumococcal and influenza vaccines are administered as a preventive measure against otitis media, nevertheless, these vaccines are only beneficial in preventing carriage and/or disease caused by vaccine serotypes. Otitis media caused by non-vaccine serotype pneumococci, non-typeable H. influenza, and M. catarrhalis remain an important healthcare burden. The development of multi-species vaccines is an arduous process but is required to reduce the global burden of this disease. Many novel vaccines against S. pneumoniae, non-typeable H. influenza, and M. catarrhalis are in preclinical trials. It is anticipated that these vaccines will lower the disease burden and provide better protection against otitis media. To study disease pathology the rat, mouse, and chinchilla are commonly used to induce experimental acute otitis media to test new therapeutics, including antibiotics and vaccines. Each of these models has its advantages and disadvantages, yet there is still a need to develop an improved animal model providing a better correlated mechanistic understanding of human middle ear infections, thereby underpinning the development of more effective otitis media therapeutics. This review provides an updated summary of current vaccines against otitis media, various animal models of otitis media, their limitations, and some future insights in this field providing a springboard in the development of new animal models and novel vaccines for otitis media.
Age-related positivity effect in emotional memory consolidation from middle age to late adulthood
Authors: Niu Xinran , Utayde Mia F. , Sanders Kristin E. G. , Denis Dan , Kensinger Elizabeth A. , Payne Jessica D.
Published: January 2024

Background

While younger adults are more likely to attend to, process, and remember negative relative to positive information, healthy older adults show the opposite pattern. The current study evaluates when, exactly, this positivity shift begins, and how it influences memory performance for positive, negative, and neutral information.

Methods

A total of 274 healthy early middle-aged (35–47), late middle-aged (48–59), and older adults (>59) viewed scenes consisting of a negative, positive, or a neutral object placed on a plausible neutral background, and rated each scene for its valence and arousal. After 12 h spanning a night of sleep (n = 137) or a day of wakefulness (n = 137), participants completed an unexpected memory test during which they were shown objects and backgrounds separately and indicated whether the scene component was the “same,” “similar,” or “new” to what they viewed during the study session.

Results and conclusions

We found that both late middle-aged and older adults rated positive and neutral scenes more positively compared to early middle-aged adults. However, only older adults showed better memory for positive objects relative to negative objects, and a greater positive memory trade-off magnitude (i.e., remembering positive objects at the cost of their associated neutral backgrounds) than negative memory trade-off magnitude (i.e., remembering negative objects at the cost of their associated neutral backgrounds). Our findings suggest that while the positivity bias may not emerge in memory until older adulthood, a shift toward positivity in terms of processing may begin in middle age.
Listening to your partner: serotonin increases male responsiveness to female vocal signals in mice
Authors: Hood Kayleigh E. , Hurley Laura M.
Published: January 2024

The context surrounding vocal communication can have a strong influence on how vocal signals are perceived. The serotonergic system is well-positioned for modulating the perception of communication signals according to context, because serotonergic neurons are responsive to social context, influence social behavior, and innervate auditory regions. Animals like lab mice can be excellent models for exploring how serotonin affects the primary neural systems involved in vocal perception, including within central auditory regions like the inferior colliculus (IC). Within the IC, serotonergic activity reflects not only the presence of a conspecific, but also the valence of a given social interaction. To assess whether serotonin can influence the perception of vocal signals in male mice, we manipulated serotonin systemically with an injection of its precursor 5-HTP, and locally in the IC with an infusion of fenfluramine, a serotonin reuptake blocker. Mice then participated in a behavioral assay in which males suppress their ultrasonic vocalizations (USVs) in response to the playback of female broadband vocalizations (BBVs), used in defensive aggression by females when interacting with males. Both 5-HTP and fenfluramine increased the suppression of USVs during BBV playback relative to controls. 5-HTP additionally decreased the baseline production of a specific type of USV and male investigation, but neither drug treatment strongly affected male digging or grooming. These findings show that serotonin modifies behavioral responses to vocal signals in mice, in part by acting in auditory brain regions, and suggest that mouse vocal behavior can serve as a useful model for exploring the mechanisms of context in human communication.
The global state of research in stem cells therapy for spinal cord injury (2003–2022): a visualized analysis
Authors: Chen Taoyu , Zhu Jiaying , Wang Gang , Sun Jinlei , Ma Xiaofeng , Tian Lijun , Zhang Meiling , Wang Fengyan , Yu Ze
Published: January 2024

Objective

Our study aimed to visualize the global status and frontiers in stem cell therapy for spinal cord injury by using bibliometric methodology.

Methods

Publication citation information related to stem cell therapy for spinal cord injury (SCI) studies between 2003 and 2022 was retrieved from the Web of Science Core Collection database. For the visualized study, VOS viewer software and Graph Pad Prism 9.5 were used to perform bibliometric analysis of included data and publication number statistics in stem cell therapy for the SCI domain.

Results

A total of 6,686 publications were retrieved. The USA and China made the highest contributions to global research with the highest number of citations and link strength. The journal Experimental Neurology ranks as the top journal, combining the publication amount and bibliometrics results. The University of Toronto, based in Canada, was the first-ranking institution. The directions of the current study could be divided into five clusters. The research of Transplantation and Regenerative Medicine and Neurosciences Mechanism Research may be the emerging frontiers in this domain.

Conclusion

In summary, stem cell therapy for spinal cord injuries is poised for more valuable advances.
The projection from dorsal medial prefrontal cortex to basolateral amygdala promotes behaviors of negative emotion in rats
Authors: Cai Youqing , Ge Jianlong , Pan Zhizhong Z.
Published: January 2024

Brain circuits between medial prefrontal cortex (mPFC) and amygdala have been implicated in cortical control of emotion, especially anxiety. Studies in recent years focus on differential roles of subregions of mPFC and amygdala, and reciprocal pathways between mPFC and amygdala in regulation of emotional behaviors. It has been shown that, while the projection from ventral mPFC to basomedial amygdala has an anxiolytic effect, the reciprocal projections between dorsal mPFC (dmPFC) and basolateral amygdala (BLA) are generally involved in an anxiogenic effect in various conditions with increased anxiety. However, the function of the projection from dmPFC to BLA in regulation of general emotional behaviors under normal conditions remains unclear. In this study, we used optogenetic analysis to identify how this dmPFC–BLA pathway regulates various emotional behaviors in normal rats. We found that optogenetic stimulation of the dmPFC–BLA pathway promoted a behavioral state of negative emotion, increasing anxiety-like and depressive-like behaviors and producing aversive behavior of place avoidance. Conversely, optogenetic inhibition of this pathway produced opposite effects, reducing anxiety-like and depressive-like behaviors, and inducing behaviors of place preference of reward. These findings suggest that activity of the dmPFC–BLA pathway is sufficient to drive a negative emotion state and the mPFC–amygdala circuit is tonically active in cortical regulation of emotional behaviors.
Causal relationship of cereal intake and type with cardiovascular disease: a Mendelian randomization study
Authors: Liu Jianhui , Cai Dihui
Published: January 2024

Background

Observational studies have suggested that cereal consumption is associated with a reduced risk of cardiovascular disease (CVD). However, the potential causal relationship is not clear. We aimed to investigate the association of cereal intake and cereal type with CVD risk.

Methods

Two-step Mendelian randomization (MR) analysis was performed to confirm the causal association of cereal intake and cereal type with the risk of several common CVDs. Furthermore, two-step MR analysis was used to explore the mediating effect of cardiovascular metabolic factors, and multivariable MR analysis was used to assess the impact of socioeconomic status, such as education and income, on the causal association.

Results

The MR analysis indicated that genetically predicted cereal intake is associated with reduced risk of large artery stroke (LAS) (odd ratio (OR): 0.421; 95% confidence interval (CI) [0.193, 0.918]; p = 0.030), and muesli as the primary cereal intake is associated with reduced risk of coronary heart disease (CHD) (OR: 0.100; 95% CI [0.023, 0.437]; p = 0.002), myocardial infarction (MI) (OR: 0.101; 95% CI [0.020, 0.509]; p = 0.005), heart failure (OR: 0.210; 95% CI [0.064, 0.684]; p = 0.010), ischemic stroke (IS) (OR: 0.130; 95% CI [0.029, 0.591]; p = 0.008), LAS (OR: 0.017; 95% CI [0.0004, 0.737]; p = 0.034), and small-vessel stroke (OR: 0.021; 95% CI [0.001, 0.708]; p = 0.005). In contrast, genetically predicted biscuits as the primary cereal intake increased the risk of CHD (OR: 6.557; 95% CI [1.197, 36.031]; p = 0.031), and other cereals, such as cornflakes, as the primary cereal intake increased the risk of CHD (OR: 3.803; 95% CI [1.194, 12.111]; p = 0.024), MI (OR: 4.240; 95% CI [1.185, 15.174]; p = 0.026), stroke (OR: 3.154; 95% CI [1.070, 9.298]; p = 0.037), and IS (OR: 3.736; 95% CI [1.185, 11.782]; p = 0.024). Multivariable MR analysis underscored the significant role of education and income in the causal association, and two-step MR analysis indicated that body mass index, lipids, and blood glucose exerted important mediating effects in the causal association.

Conclusion

The findings of our study underscore the causal beneficial influence of muesli as the primary cereal intake on CVDs. A reasonable consumption of muesli may provide primary prevention of CVDs.
Unveiling diagnostic and therapeutic strategies for cervical cancer: biomarker discovery through proteomics approaches and exploring the role of cervical cancer stem cells
Authors: Jafari Ameneh , Farahani Masoumeh , Abdollahpour-Alitappeh Meghdad , Manzari-Tavakoli Asma , Yazdani Mohsen , Rezaei-Tavirani Mostafa
Published: January 2024

Cervical cancer (CC) is a major global health problem and leading cause of cancer deaths among women worldwide. Early detection through screening programs has reduced mortality; however, screening compliance remains low. Identifying non-invasive biomarkers through proteomics for diagnosis and monitoring response to treatment could improve patient outcomes. Here we review recent proteomics studies which have uncovered biomarkers and potential drug targets for CC. Additionally, we explore into the role of cervical cancer stem cells and their potential implications in driving CC progression and therapy resistance. Although challenges remain, proteomics has the potential to revolutionize the field of cervical cancer research and improve patient outcomes.
Treatment decision for recurrences in non-small cell lung cancer during or after adjuvant osimertinib: an international Delphi consensus report
Authors: Mirza Myriam , Shrivastava Aseem , Matthews Cecile , Leighl Natasha , Ng Calvin S. H. , Planchard David , Popat Sanjay , Rotow Julia , Smit Egbert F. , Soo Ross , Tsuboi Masahiro , Yang Fan , Stiles Brendon , Grohe Christian , Wu Yi-Long
Published: January 2024

Introduction

Osimertinib is recommended by major guidelines for use in the adjuvant setting in patients with EGFR mutation-positive NSCLC following the significant improvement in disease-free survival observed in the Phase III ADAURA trials. Due to limited real-world data in the adjuvant setting, little guidance exists on how to approach potential recurrences either during or after the completion of the treatment. This study aimed to reach a broad consensus on key treatment decision criteria in the events of recurrence.

Methods

To reach a broad consensus, a modified Delphi panel study was conducted consisting of two rounds of surveys, followed by two consensus meetings and a final offline review of key statements. An international panel of experts in the field of NSCLC (n=12) was used to provide clinical insights regarding patient management at various stages of NSCLC disease including patient monitoring, diagnostics, and treatment approach for specific recurrence scenarios. This study tested recurrences occurring 1) within or outside the central nervous system (CNS), 2) during or after the adjuvant-osimertinib regimen in NSCLC disease which is 3) amenable or not amenable to local consolidative therapy.

Results

Panellists agreed on various aspects of patient monitoring and diagnostics including the use of standard techniques (e.g., CT, MRI) and tumour biomarker assessment using tissue and liquid biopsies. Consensus was reached on 6 statements describing treatment considerations for the specific NSCLC recurrence scenarios. Panellists agreed on the value of osimertinib as a monotherapy or as part of the overall treatment strategy within the probed recurrence scenarios and acknowledged that more clinical evidence is required before precise recommendations for specific patient populations can be made.

Discussion

This study provides a qualitative expert opinion framework for clinicians to consider within their treatment decision-making when faced with recurrence during or after adjuvant-osimertinib treatment.
Sex-specific molecular differences in glioblastoma: assessing the clinical significance of genetic variants
Authors: Jovanovich Nicolina , Habib Ahmed , Chilukuri Akanksha , Hameed N. U. Farrukh , Deng Hansen , Shanahan Regan , Head Jeffrey R. , Zinn Pascal O.
Published: January 2024

Introduction

Glioblastoma multiforme (GBM) is one of the most aggressive types of brain cancer, and despite rigorous research, patient prognosis remains poor. The characterization of sex-specific differences in incidence and overall survival (OS) of these patients has led to an investigation of the molecular mechanisms that may underlie this dimorphism.

Methods

We reviewed the published literature describing the gender specific differences in GBM Biology reported in the last ten years and summarized the available information that may point towards a patient-tailored GBM therapy.

Results

Radiomics analyses have revealed that imaging parameters predict OS and treatment response of GBM patients in a sex-specific manner. Moreover, gender-based analysis of the transcriptome GBM tumors has found differential expression of various genes, potentially impacting the OS survival of patients in a sex-dependent manner. In addition to gene expression differences, the timing (subclonal or clonal) of the acquisition of common GBM-driver mutations, metabolism requirements, and immune landscape of these tumors has also been shown to be sex-specific, leading to a differential therapeutic response by sex. In male patients, transformed astrocytes are more sensitive to glutaminase 1 (GLS1) inhibition due to increased requirements for glutamine uptake. In female patients, GBM is more sensitive to anti-IL1β due to an increased population of circulating granulocytic myeloid-derived suppressor cells (gMDSC).

Conclusion

Moving forward, continued elucidation of GBM sexual dimorphism will be critical in improving the OS of GBM patients by ensuring that treatment plans are structured to exploit these sex-specific, molecular vulnerabilities in GBM tumors.
A Mendelian analysis of the relationships between immune cells and breast cancer
Authors: Wang Xin , Gao Haoyu , Zeng Yiyao , Chen Jie
Published: January 2024

Background

Emerging evidence showed immune cells were associated with the development of breast cancer. Nonetheless, the causal link between them remains uncertain. Consequently, the objective of this study was to investigate the causal connection between immune traits and the likelihood of developing breast cancer.

Methods

A two-sample Mendelian randomization (MR) analysis was conducted to establish the causal relationship between immune cells and breast cancer in this study. Utilizing publicly accessible genetic data, we investigated causal connections between 731 immune cells and the occurrence of breast cancer. The primary approach for exploring this relationship was the application of the inverse-variance-weighted (IVW) method. Furthermore, sensitivity analyses, encompassing the leave-one-out analysis, Cochran Q test, and Egger intercept test were performed to validate the reliability of the Mendelian randomization results. Finally, we used Bayesian Weighted Mendelian Randomization (BWMR) approach to test the results of MR study.

Results

According to the Bonferroni correction, no immune trait was identified with a decreased or increased risk of overall breast cancer risk. As for the ER+ breast cancer, 6 immune trait was identified after the Bonferroni method. the IVW method results showed that CD45RA- CD4+ %CD4+ (p-value:1.37×10−6), CD8dim %T cell (p-value:4.62×10−43), BAFF-R on IgD+ CD38- unsw mem (p-value:6.93×10−5), CD27 on PB/PC (p-value:2.72×10−18) lowered the risk of breast cancer. However, CD19 on IgD- CD38br (p-value:1.64×10−6), CD25 on IgD+ CD38dim (p-value: - ∞) were associated with a higher risk of developing breast cancer. As for the CX3CR1 on CD14+ CD16- monocyte (p-value: 1.15×10−166), the IVW method clearly demonstrated a protective effect against ER- breast cancer. For the above positive results, BAFF-R on IgD+ CD38- unsw mem was the sole association linked to reduced breast cancer risk using the BWMR method. The intercept terms’ p-values in MR-Egger regression all exceeded 0.05, indicating the absence of potential horizontal pleiotropy.

Conclusion

Through genetic approaches, our study has illustrated the distinct correlation between immune cells and breast cancer, potentially paving the way for earlier diagnosis and more efficient treatment alternatives.
Therapeutic evolution in HR+/HER2- breast cancer: from targeted therapy to endocrine therapy
Authors: Cao Lu-Qi , Sun Haidong , Xie Yuhao , Patel Harsh , Bo Letao , Lin Hanli , Chen Zhe-Sheng
Published: January 2024

Breast cancer, a complex and varied disease, has four distinct subtypes based on estrogen receptor and human epidermal growth factor receptor 2 (HER2) levels, among which a significant subtype known as HR+/HER2-breast cancer that has spurred numerous research. The prevalence of breast cancer and breast cancer-related death are the most serious threats to women’s health worldwide. Current progress in treatment strategies for HR+/HER2-breast cancer encompasses targeted therapy, endocrine therapy, genomic immunotherapy, and supplementing traditional methods like surgical resection and radiotherapy. This review article summarizes the current epidemiology of HR+/HER2-breast cancer, introduces the classification of HR+/HER2-breast cancer and the commonly used treatment methods. The mechanisms of action of various drugs, including targeted therapy drugs and endocrine hormone therapy drugs, and their potential synergistic effects are deeply discussed. In addition, clinical trials of these drugs that have been completed or are still in progress are included.