bluuebunny/crossref_metadata_2025 huggingface dataset:
Cascading renal injury after brain death: Unveiling glycocalyx alteration and the potential protective role of tacrolimus
Authors: Idouz Kaoutar , Belhaj Asmae , Rondelet Benoit , Dewachter Laurence , Flamion Bruno , Kirschvink Nathalie , Dogné Sophie
Published: August 2024

Brain death (BD) is a complex medical state that triggers systemic disturbances and a cascade of pathophysiological processes. This condition significantly impairs both kidney function and structural integrity, thereby presenting considerable challenges to graft viability and the long-term success of transplantation endeavors. Tacrolimus (FK506), an immunosuppressive drug, was used in this study to assess its impact as a pretreatment on brain death-induced renal injury. This study aimed to investigate changes associated with brain death-induced renal injury in a 4-month-old female porcine model. The experimental groups included brain death placebo-pretreated (BD; n = 9), brain death tacrolimus-pretreated using the clinical dose of 0.25 mg/kg the day before surgery, followed by 0.05 mg/kg/day 1 hour before the procedure (BD + FK506; n = 8), and control (ctrl, n = 7) piglets, which did not undergo brain death induction. Furthermore, we aimed to assess the effect of FK506 on these renal alterations through graft preconditioning. We hypothesized that immunosuppressive properties of FK506 reduce tissue inflammation and preserve the glycocalyx. Our findings revealed a series of interconnected events triggered by BD, leading to a deterioration of renal function and increased proteinuria, increased apoptosis in the vessels, glomeruli and tubules, significant leukocyte infiltration into renal tissue, and degradation of the glycocalyx in comparison with ctrl group. Importantly, treatment with FK506 demonstrated significant efficacy in attenuating these adverse effects. FK506 helped reduce apoptosis, maintain glycocalyx integrity, regulate neutrophil infiltration, and mitigate renal injury following BD. This study offers new insights into the pathophysiology of BD-induced renal injury, emphasizing the potential of FK506 pretreatment as a promising therapeutic intervention for organ preservation, through maintaining endothelial function with the additional benefit of limiting the risk of rejection.
Structural insights of an LCP protein–LytR–from Streptococcus dysgalactiae subs. dysgalactiae through biophysical and in silico methods
Authors: Paquete-Ferreira João , Freire Filipe , Fernandes Henrique S. , Muthukumaran Jayaraman , Ramos João , Bryton Joana , Panjkovich Alejandro , Svergun Dmitri , Santos Marino F. A. , Correia Márcia A. S. , Fernandes Alexandra R. , Romão Maria João , Sousa Sérgio F. , Santos-Silva Teresa
Published: August 2024

The rise of antibiotic-resistant bacterial strains has become a critical health concern. According to the World Health Organization, the market introduction of new antibiotics is alarmingly sparse, underscoring the need for novel therapeutic targets. The LytR-CpsA-Psr (LCP) family of proteins, which facilitate the insertion of cell wall glycopolymers (CWGPs) like teichoic acids into peptidoglycan, has emerged as a promising target for antibiotic development. LCP proteins are crucial in bacterial adhesion and biofilm formation, making them attractive for disrupting these processes. This study investigated the structural and functional characteristics of the LCP domain of LytR from Streptococcus dysgalactiae subsp. dysgalactiae. The protein structure was solved by X-ray Crystallography at 2.80 Å resolution. Small-angle X-ray scattering (SAXS) data were collected to examine potential conformational differences between the free and ligand-bound forms of the LytR LCP domain. Additionally, docking and molecular dynamics (MD) simulations were used to predict the interactions and conversion of ATP to ADP and AMP. Experimental validation of these predictions was performed using malachite green activity assays. The determined structure of the LCP domain revealed a fold highly similar to those of homologous proteins while SAXS data indicated potential conformational differences between the ligand-free and ligand-bound forms, suggesting a more compact conformation during catalysis, upon ligand binding. Docking and MD simulations predicted that the LytR LCP domain could interact with ADP and ATP and catalyze their conversion to AMP. These predictions were experimentally validated by malachite green activity assays, confirming the protein’s functional versatility. The study provides significant insights into the structural features and functional capabilities of the LCP domain of LytR from S. dysgalactiae subsp. dysgalactiae. These findings pave the way for designing targeted therapies against antibiotic-resistant bacteria and offer strategies to disrupt bacterial biofilm formation.
Exploring the causality of appendectomy and ischaemic heart disease: a Mendelian randomization study and meta-analysis
Authors: Wang Shuai , Zhang Tao , Sun Yuanlin , Yao Yiwei , Yang Dongliang , Cao Xueyuan
Published: August 2024

Background

The risk of ischaemic heart disease (IHD) is increased in appendectomy patients, but it is not clear whether there is a causal relationship. We aimed to systematically estimate the causal relationship between appendectomy and IHD and its subtypes, acute myocardial infarction (AMI) and angina pectoris (AP), using Mendelian randomization (MR) study methods and meta-analysis.

Methods

As the discovery cohort analysis, we extracted independent genetic variants strongly associated with appendectomy from the FinnGen study (28,601 cases) as instrumental variables (IVs). Genome-wide association study (GWAS) from UK Biobank were selected for outcome data. A first two-sample MR analysis was then conducted. As the replication cohort, IVs associated with appendectomy were extracted in the UK Biobank (50,105 cases). GWAS from the FinnGen study were selected for outcome data. A second MR analysis was then performed. Finally, meta-analyses were applied to assess the combined causal effects of the MR results.

Results

In the discovery cohort, there was a significant positive causal relationship between appendectomy and IHD and its subtypes AMI and AP. The replication cohort only found a positive causal relationship between appendectomy and AMI. Meta-analysis showed a positive causal relationship between appendectomy and IHD (OR: 1.128, 95% CI: 1.067–1.193, P = 2.459e-05), AMI (OR: 1.195, 95% CI: 1.095–1.305, P = 6.898e-05), and AP (OR: 1.087, 95% CI: 1.016–1.164, P = 1.598e-02).

Conclusions

This comprehensive MR analysis suggests that genetically predicted appendectomy may be a risk factor for the development of IHD and its subtypes AMI and AP. We need to continue to pay attention to these links.
A comparative evaluation of the leadership development needs of basic school leaders in the 4.0 era
Authors: Awodiji Omotayo A. , Naicker Suraiya R.
Published: August 2024

School leadership development (SLP) in the context of the Fourth Industrial Revolution (4.0) is a crucial aspect of preparing basic school leaders to navigate the challenges and opportunities presented by the rapid technological advancements and changes in the modern world. The 4.0 is characterized by the fusion of digital, physical, and biological technologies, which profoundly impact various aspects of society, including education. Thus, this study examined basic school leaders’ development needs in 4.0 in Nigeria and South Africa. A comparative research approach of survey design was adopted with the aid of a questionnaire. Descriptive statistics (mean score) and an independent t-test were used to analyze the collected data. Results indicated that basic school leaders in South Africa fared better than their counterparts from Nigeria regarding their 4.0 knowledge. However, no significant difference was found in 4.0 leadership skills. Leaders from both nations indicated the need for development programs in technological applications to enhance competency to perform their administrative tasks. In theory, the study addresses African schools’ 4.0 leadership needs. To lead education into the future, each nation must also understand the specific 4.0 leadership skills its school leaders will need. In light of the study’s findings, policymakers should consider what gaps need to be filled to equip their basic school leaders with Education 4.0 skills.
Development and validation of a predictive nomogram for the risk of MAFLD in postmenopausal women
Authors: Yang Ming , Chen Xingyu , Shen Qiaohui , Xiong Zhuang , Liu Tiejun , Leng Yan , Jiao Yue
Published: August 2024

Background and aim

Metabolic-associated fatty liver disease (MAFLD) has gradually become one of the main health concerns regarding liver diseases. Postmenopausal women represent a high-risk group for MAFLD; therefore, it is of great importance to identify and intervene with patients at risk at an early stage. This study established a predictive nomogram model of MAFLD in postmenopausal women and to enhance the clinical utility of the new model, the researchers limited variables to simple clinical and laboratory indicators that are readily obtainable.

Methods

Data of 942 postmenopausal women from January 2023 to October 2023 were retrospectively collected and divided into two groups according to the collection time: the training group (676 cases) and the validation group (226 cases). Significant indicators independently related to MAFLD were identified through univariate logistic regression and stepwise regression, and the MAFLD prediction nomogram was established. The C-index and calibration curve were used to quantify the nomogram performance, and the model was evaluated by measuring the area under the receiver operating characteristic curve (AUC), calibration curve, and decision curve analysis (DCA).

Results

Of 37 variables, 11 predictors were identified, including occupation (worker), body mass index, waist-to-hip ratio, number of abortions, anxiety, hypertension, hyperlipidemia, diabetes, hyperuricemia, and diet (meat and processed meat). The C-index of the training group predicting the related risk factors was 0.827 (95% confidence interval [CI] 0.794–0.860). The C-index of the validation group was 0.787 (95% CI 0.728–0.846). Calibration curves 1 and 2 (BS1000 times) were close to the diagonal, showing a good agreement between the predicted probability and the actual incidence in the two groups. The AUC of the training group was 0.827, the sensitivity was 0.784, and the specificity was 0.735. The AUC of the validation group was 0.787, the sensitivity was 0.674, and the specificity was 0.772. The DCA curve showed that the nomogram had a good net benefit in predicting MAFLD in postmenopausal women.

Conclusions

A predictive nomogram for MAFLD in postmenopausal women was established and verified, which can assist clinicians in evaluating the risk of MAFLD at an early stage.
Enhanced recovery after surgery in elective cesarean section patients with gestational diabetes mellitus does not lead to glucose-related maternal and neonatal complications
Authors: Zhou Jin , Zhang Peizhen , Tan Zhangmin , Li Chuo , Yao Lin , He Tiantian , Hou Hongyin , Yin Yuzhu
Published: August 2024

Objective

For elective cesarean section patients with gestational diabetes mellitus (GDM), there is a lack of evidence-based research on the use of enhanced recovery after surgery (ERAS). This study aims to compare the ERAS after-surgery protocol and traditional perioperative management.

Research design and methods

In this retrospective cohort study, singleton pregnancies with good glucose control GDM, delivered by elective cesarean sections under intravertebral anesthesia at least 37 weeks from January 1 to December 31, 2022, were collected at the Third Affiliated Hospital of Sun Yat-sen University. We divided all enrolled pregnant women and newborns into an ERAS group and a control group (the traditional perioperative management group) based on their adherence to the ERAS protocol. The primary outcome was the preoperative blood glucose level, with an increase of more than 1 mmol/L indicating clinical significance when compared to the control group. The secondary outcome was centered around an adverse composite outcome that affected both mothers and newborns.

Results

We collected a total of 161 cases, with 82 in the ERAS group and 79 in the control group. Although the mean preoperative blood glucose level in the ERAS group was significantly higher than in the control group (5.01 ± 1.06 mmol/L vs. 4.45 ± 0.90 mmol/L, p<0.001), the primary outcome revealed that the mean glycemic difference between the groups was 0.47 mmol/L (95% CI 0.15-0.80 mmol/L), which was below the clinically significant difference of 1 mmol/L. For the secondary outcomes, the ERAS group had an 86% lower risk of a composite adverse outcome compared to the control group. This included a 73% lower risk of perioperative maternal hypoglycemia and a 92% lower rate of neonatal hypoglycemia, all adjusted by age, hypertensive disorder of pregnancy, BMI, gestational weeks, primigravidae, primary pregnancy, GDM, surgery duration, and fasting glucose.

Conclusion

Implementing a low-dose carbohydrate ERAS in pregnant women with GDM prior to elective cesarean section, compared to traditional perioperative management, does not lead to clinically significant maternal glucose increases and thus glucose-related maternal or neonatal perioperative complications.
Microwave ablation for lymph node metastasis in thyroid cancer: the impact of lymph node diameter
Authors: Xiao Xiaoyi , Chen Xi , Li Jingwei , Li Pei , Zhu Yun
Published: August 2024

Objectives

To explore the impact of lymph node diameter on the efficacy and safety of ultrasound-guided microwave ablation (MWA) in the treatment of cervical metastatic lymph nodes (CMLNs) from thyroid cancer.

Methods

A total of 32 patients with 58 CMLNs from thyroid cancer underwent ultrasound-guided MWA and were included in the retrospective study. Patients were divided into three groups based on the mean largest diameter of the CMLNs: Group A (diameter ≤10mm), Group B (10mm < diameter ≤20mm), and Group C (diameter >20mm). The research involved comparing changes in cervical metastatic lymph nodes and serum thyroglobulin (sTg) levels, as well as the incidence of complications, before and after microwave ablation across three groups of patients.

Results

The technical success rate of this study was 100% (32/32), and they showed no major complications. Compared with measurements taken before MWA, the mean largest diameter and volume of CMLNs, as well as the sTg level, showed significant reductions (p <0.05) at the last follow-up in all three patient groups. Group A and B exhibited higher lymph node volume reduction rates and complete disappearance rates compared to Group C. However, the recurrence rate in the three groups were in the following order: Group C > Group B > Group A. The occurrence rate of mild complications was Group A > Group C > Group B.

Conclusion

MWA is a safe and effective method for treating CMLNs, with advantages for localized nodes but limitations for larger ones. Careful consideration and personalized plans are advised, based on comprehensive evidence assessment.
Paraben residues in wastewater and surface water: a case study of KwaZulu Natal and Gauteng provinces (South Africa) during the COVID-19 pandemic
Authors: Mpayipheli Neliswa , Mpupa Anele , Madala Ntakadzeni Edwin , Nomngongo Philiswa Nosizo
Published: August 2024

Introduction: The presence of pharmaceuticals and personal care products in environmental matrices is considered one of the major scientific concerns. Most of these substances are disposed of unchanged through wastewater treatment plants and sewage systems. Consequently, they are continuously introduced into the water systems and progressively contaminate surface, ground and drinking water. During the COVID-19 pandemic, a large number of emerging contaminants including parabens were released to the environment through various routes. In this study, the occurrence of parabens (methylparaben (MePB), ethylparaben (EtPB), propylparaben (PrPB), and butylparaben (BuPB) was investigated in wastewater samples from various wastewater treatment plants (WWTPs) and the receiving surface waters in KwaZulu Natal and Gauteng Provinces (South Africa).

Methods: The samples were collected between October 2020 and December 2021, covering the 2nd, 3rd and 4th waves of the COVID-19 pandemic. A solid phase extraction protocol with high-performance liquid chromatography was used to extract and enrich parabens before analysis.

Results and Discussion: Methylparaben (2.02–84.7 μg/L), EtPB (<0.24–24.8 μg/L), PrPB (<0.26–55.1 μg/L), and BuPB (<0.27–17.3 μg/L) were quantified in wastewater influent collected WWTPs of KwaZulu Natal Province. While <0.19–5.43 μg/L, <0.16–5.63 μg/L, <0.17–6.89 μg/L, and <0.19–5.32 μg/L for MePB, EtPB, PrPB, and BuPB, respectively, were quantified in effluent wastewater from the same province. The concentrations of MePB, EtPB, PrPB, and BuPB in influent wastewater from Gauteng Province were 2.58–123 μg/L, <0.24–33.6 μg/L, 3.77–73.4 μg/L and <0.27–85.8 μg/L, respectively. In effluent wastewater, concentrations ranging from 0.24–17.76 μg/L (MePB), <0.16–4.88 μg/L (EtPB), 0.69-12.5 μg/L (PrPB), and <0.19–4.726 μg/L (BuPB) were quantified. During the 4th wave, the concentrations of parabens in surface water were lower compared to the second and third waves of the pandemic. In general, the paraben residues in the surface of KwaZulu Natal Province (<0.08–16.4 μg/L) were higher than those in Gauteng Province (0.08-3.14 µg/L). Methylparaben and propylparaben were dominant in all investigated samples (wastewater and surface water), followed by ethylparaben. The ecotoxicological risk assessment was carried out for aquatic biota, which was estimated in terms of risk quotients (RQs). RQs for the target compounds in river water indicated that MePB and EtPB pose low risk, whereas PrPB and BuPB pose low to medium risk to aquatic organisms.
Fire behavior simulation of Xintian forest fire in 2022 using WRF-fire model
Authors: Hu Hongmei , Deng Xiangwen , Zhang Gui , Feng Lanbo , Long Jun , Li Ziming , Zhu Yu , Wang Yiying
Published: August 2024

Introduction

The behavior of forest fire is a complex phenomenon, and accurate simulation of forest fire is conducive to emergency response management after ignition. In order to further understand the characteristics of forest fire spread and the applicability of WRF-Fire in China, which is a coupled fire-atmospheric wildfire model, this study simulated a high-intensity forest fire event that occurred on October 17, 2022 in Xintian County, southern Hunan Province.

Methods

Based on the fire-atmosphere coupled WRF-Fire model, we used high-resolution geographic information, meteorological observation and fuel classification data to analyze the forest fire behavior. At the same time, the simulation results are compared with the fire burned area observed by satellite remote sensing forest fire monitoring data.

Results

The study found that, the simulated wind speed, direction and temperature trends are similar to the observation results, but the simulated wind speed is overestimated, the dominant wind direction is N, and the temperature is slightly underestimated. The simulated wind field is close to the actual wind field, and the simulation results can show the spatial and temporal variation characteristics of the local wind field under complex terrain while obtaining the high-resolution wind field. The simulated fire burned area is generally overestimated, spreading to the north and southwest compared with the observed fires, but it can also capture the overall shape and spread trend of the fire well.

Discussion

The results show that the model can accurately reproduce the real spread of fire, and it is more helpful to forest fire management.
Abundance, diversity and composition of understory plants along the altitudinal gradient and dominant overstory composition types in the temperate Himalayan region
Authors: Sangry Saveena , Kumar Praveen , Bhardwaj D.R. , Dogra K.S. , Poonam
Published: August 2024

Introduction

The Indian Himalayan forests are remarkable landforms experiencing tremendous climatic variation, constituting complex and diversified ecosystems with prominent vegetation zones. Despite their global significance and substantial research efforts focused on plant diversity in the temperate Himalayan region, only a few studies have explicitly assessed the distribution patterns of understory vegetation in relation to forest compositional types along altitudinal gradients.

Methods

To cover a wide range of altitudes and diverse overstory compositions, stands were sampled across four altitudinal ranges from 1500 to 3500 meters above mean sea level with increments of 500 meters in elevation steps. The overstory compositions were classified on the basis of dominant tree species in each stand on the similar sites. Vegetation in the shrub and ground layers was surveyed by visually estimating the percentage coverage within circular plots.

Results

A total of 99 understory species including 37 species each in the shrub layer, 62 species in the herb layer vegetation were recorded. The abundance, species diversity and composition of understory vegetation differed significantly along the altitudinal gradient and dominant overstory composition types. Moreover, distinct understory vegetation communities were observed at lower elevations compared to higher elevations, with middle elevations exhibiting intermediate vegetation characteristics. The study also highlighted the importance of dominant overstory composition types in shaping the pattern of understory vegetation abundance, species diversity and composition in the temperate Himalayan region. The higher resource conditions associated with broadleaved stands supported higher understory species abundance at lower elevations, while the heterogeneous conditions induced by the mixedwood stands promoted higher understory species diversity.

Conclusion

The hump shaped pattern along the altitudinal gradient appeared to be the most dominant pattern of plant abundance and species diversity and call for more conservation concern towards the middle elevation zones in the temperate Himalayan region. Furthermore, the management interventions should aim at maintaining diverse range of overstory composition types for conserving biodiversity and their ecological functions in the temperate Himalayan region.
Factors influencing fish migration in one of the world's largest inland fisheries
Authors: Sor Ratha , Prudencio Liana , Hogan Zeb S. , Chandra Sudeep , Ngor Peng Bun , Null Sarah E.
Published: August 2024

Fish from Cambodia's Tonle Sap Lake are economically, culturally, and nutritionally significant for people in the Lower Mekong Basin, providing income, livelihoods, and protein. Fish in this system generally migrate toward upstream Mekong River in dry season and return in early wet season. However, drivers of fish migration from Tonle Sap Lake to the Mekong River are not well-understood. In this paper, we utilized Mixed Effects Random Forest to predict the catch weight of six fish species migrating from the Tonle Sap Lake to the Mekong River using precipitation, lunar cycle, and hydrologic conditions like river stage, streamflow, flow magnitude, and timing as predictors. As a surrogate for fish migration, we used daily fish catch weight from 2002 through 2008 at the bagnet, or Dai, fisheries along Tonle Sap River, a migration corridor connecting Tonle Sap Lake to the Mekong River. We found that migration of large fish was mainly cued by streamflow and flow magnitude, while smaller fish migrate depending on the combination of streamflow and flow timing. Streamflow less than average cumulative flow was the most important driver for migration of Pangasianodon hypophthalmus, and Cirrhinus microlepis. Migration of Cyclocheilichthys enoplos and Osteochilus melanopleurus was highly dependent on the number of low- and minimum-flow days. Cumulative flows, period of high flow and water level were the main predictors of the small mud-carp Henicorhynchus entmema's migration, while individuals of Labiobarbus leptocheilus migrated out of the Tonle Sap Lake depending on the number of days after 7-, 30-, and 90-day minimum flows. These results suggest that flow characteristics can be used to aid conservation and adaptive management of Cambodia's Dai fisheries.
The modification role and tumor association with a methyltransferase: KMT2C
Authors: Jiao Yunjuan , Lv Yuanhao , Liu Mingjie , Liu Yun , Han Miaomiao , Xiong Xiwen , Zhou Hongyan , Zhong Jiateng , Kang Xiaohong , Su Wei
Published: August 2024

Histone methylation can affect chromosome structure and binding to other proteins, depending on the type of amino acid being modified and the number of methyl groups added, this modification may promote transcription of genes (H3K4me2, H3K4me3, and H3K79me3) or reduce transcription of genes (H3K9me2, H3K9me3, H3K27me2, H3K27me3, and H4K20me3). In addition, advances in tumor immunotherapy have shown that histone methylation as a type of protein post-translational modification is also involved in the proliferation, activation and metabolic reprogramming of immune cells in the tumor microenvironment. These post-translational modifications of proteins play a crucial role in regulating immune escape from tumors and immunotherapy. Lysine methyltransferases are important components of the post-translational histone methylation modification pathway. Lysine methyltransferase 2C (KMT2C), also known as MLL3, is a member of the lysine methyltransferase family, which mediates the methylation modification of histone 3 lysine 4 (H3K4), participates in the methylation of many histone proteins, and regulates a number of signaling pathways such as EMT, p53, Myc, DNA damage repair and other pathways. Studies of KMT2C have found that it is aberrantly expressed in many diseases, mainly tumors and hematological disorders. It can also inhibit the onset and progression of these diseases. Therefore, KMT2C may serve as a promising target for tumor immunotherapy for certain diseases. Here, we provide an overview of the structure of KMT2C, disease mechanisms, and diseases associated with KMT2C, and discuss related challenges.
Temporal patterns in dolphin foraging activity in the Mediterranean Sea: insights from vocalisations recorded during the ACCOBAMS Survey Initiative
Authors: Sol Morgane , Ollier Camille , Boisseau Oliver , Ridoux Vincent , Virgili Auriane
Published: August 2024

Marine organisms continually adapt their physiology and behaviour to temporal variations in their environment, resulting in diurnal rhythmic behaviour, particularly when foraging. In delphinids, these rhythms can be studied by recording echolocation clicks, which can provide indicators of foraging activity. The foraging rhythms of delphinids and their relationship to temporal parameters are poorly documented and most studies so far have used moored passive acoustic systems. The present study provides, for the first time, information on the activity rhythms of delphinids investigated in relation with temporal variables at a basin scale from a moving platform, in the western and central Mediterranean Sea. We used passive acoustic recordings collected by hydrophones towed along transect lines during the ACCOBAMS Survey Initiative in the summer 2018. We extracted variables that may influence daily and monthly rhythms, including time of day, lunar cycle, lunar illumination and sea state and fitted generalised additive models. The nycthemeral and lunar cycles were the two main factors influencing dolphin activity rhythms. Echolocation activity was predominant at night, with a maximum of 0.026 acoustic events per minute at 21:00/22:00 compared to as few as 0.0007 events per minute at 11:00. These events were also more frequent during the third quarter of the moon; 0.033 acoustic events on day 22 of the lunar cycle as opposed to 0.0008 on day 8 of the lunar cycle, corresponding to the first quarter of the moon. Variations in the echolocation activity of delphinids in the Mediterranean Sea could reflect variation in their foraging effort and be related to prey density, composition, accessibility and catchability within dolphin foraging depth range. These results should also improve interpretation of passive acoustic monitoring data.
Short-term effects of an unprecedented heatwave on intertidal bivalve populations: fisheries management surveys provide an incomplete picture
Authors: Raymond Wendel W. , Tobin Elizabeth D. , Barber Julie S. , Hayford Hilary A. , Raymond Ann E. T. , Speck Camille A. , Rogers Doug , Brown Rana
Published: August 2024

Introduction

Coastal marine ecosystems, are particularly susceptible to climate change. One such threat is atmospheric heatwaves, which are predicted to increase in frequency, duration, and intensity. Many intertidal organisms already live at the edge of their thermal tolerance limits and heatwaves can outstretch an organism’s ability to compensate in the short term. In June 2021 the Pacific Northwest region of North America, including the Salish Sea, experienced a significant atmospheric heatwave during some of the lowest tides of the year. This was followed by numerous reports of dead and dying intertidal marine organisms region-wide. A semi-quantitative rapid assessment found a range of both species- and location-specific effects but generally recorded widespread negative impacts to intertidal shellfish species across the Salish Sea.

Methods

Following these results, we opportunistically analyzed data collected by intertidal bivalve resource managers from the region. These datasets allowed us to examine regional density and size data for clam and oyster populations before and after the heatwave to increase our quantitative understanding of heatwave effects.

Results

We found a range of responses including positive and negative effects of the heatwave on clam and oyster density. While we generally found small changes in bivalve size, some site-species combinations displayed large shifts in size frequency. Many of our analyses did not indicate even moderate statistical support, even with large changes in the mean, driven in part by high variability in the data. Time intervals between surveys, ranging from 2 to over 25 months, had little effect on observed variability indicating that any heatwave-induced effects may be masked by variability inherent to the population ecology and/or survey methodology.

Discussion

This analysis has highlighted the need for intertidal resource managers, and the greater research community, to consider alternative survey approaches designed to constrain variability in order to detect the effects of acute or extreme events. With the effects of climate change predicted to become more intense, targeted survey approaches may be needed to detect the effects and implications of such events and to continue effective management of intertidal bivalves in the Salish Sea and worldwide.
Research on seamount substrate classification method based on machine learning
Authors: Huang DeXiang , Sun YongFu , Gao Wei , Xu WeiKun , Wang Wei , Zhang YiXin , Wang Lei
Published: August 2024

The western Pacific seamount area is abundant in both biological and mineral resources, making it a crucial location for international investigation of regional seabed resources. An essential stage in comprehending and advancing seamounts is gaining knowledge about the distribution characteristics and laws governing the seabed substrate. Deep-sea geological sampling is challenging because of the intricate nature of the deep-sea environment, resulting in increased difficulty in identifying and evaluating substrates. This study addresses the aforementioned issues by utilizing in-situ video footage obtained from the “Jiaolong” manned deep submersible and shipborne deep-water multibeam data. This data is used as a foundation for constructing a Western Pacific seamount areas substrate classification point set. Additionally, the paper introduces the mRMR-XGBoost substrate classification model. Substrate categorization in deep sea and mountainous regions has been successfully accomplished, yielding a classification accuracy of 92.5%. The classification experiments and box sampling results demonstrate that the mRMR-XGBoost substrate classification model proposed in this paper can efficiently use acoustic and optical data to accurately divide the substrate types in seamount areas, with better classification accuracy, when compared with commonly used machine learning models. It has a significant application value and the best classification effect on the two types of substrates: nodules and gravel substrates.
PTEN in kidney diseases: a potential therapeutic target in preventing AKI-to-CKD transition
Authors: Cao Fangfang , Li Yuanyuan , Peng Ting , Li Yuanmei , Yang Lihua , Hu Lanping , Zhang Han , Wang Jiali
Published: August 2024

Renal fibrosis, a critical factor in the development of chronic kidney disease (CKD), is predominantly initiated by acute kidney injury (AKI) and subsequent maladaptive repair resulting from pharmacological or pathological stimuli. Phosphatase and tensin homolog (PTEN), also known as phosphatase and tensin-associated phosphatase, plays a pivotal role in regulating the physiological behavior of renal tubular epithelial cells, glomeruli, and renal interstitial cells, thereby preserving the homeostasis of renal structure and function. It significantly impacts cell proliferation, apoptosis, fibrosis, and mitochondrial energy metabolism during AKI-to-CKD transition. Despite gradual elucidation of PTEN’s involvement in various kidney injuries, its specific role in AKI and maladaptive repair after injury remains unclear. This review endeavors to delineate the multifaceted role of PTEN in renal pathology during AKI and CKD progression along with its underlying mechanisms, emphasizing its influence on oxidative stress, autophagy, non-coding RNA-mediated recruitment and activation of immune cells as well as renal fibrosis. Furthermore, we summarize prospective therapeutic targeting strategies for AKI and CKD-treatment related diseases through modulation of PTEN.
AM/PM dosing of LAMA for COPD: a randomized controlled trial protocol using digital recruitment and registries
Authors: Sivapalan Pradeesh , Rømer Valdemar , Wirenfeldt Klausen Tobias , Dyrby Johansen Niklas , Pareek Manan , Modin Daniel , Mathioudakis Alexander , Vestbo Jørgen , Eklöf Josefin , Jordan Alexander , Hurst John R. , Biering-Sørensen Tor , Jensen Jens-Ulrik
Published: August 2024

Rationale

Long-acting muscarinic antagonists (LAMAs) reduce the risk of acute exacerbations of chronic obstructive pulmonary disease (AECOPD), usually taken once daily in the morning. However, the circadian activity of autonomic regulation suggests that the highest need for anticholinergic therapy may be in the late night/early morning. This is supported by evidence that AECOPD most often begins in the morning. Furthermore, the trough spirometry effect of LAMA is lower than the peak effect, which further argues that evening dosing may be more optimal than morning dosing. This trial aims to determine whether evening administration of LAMA reduces hospitalization-requiring AECOPD or death from all causes within 1 year as compared to morning administration of the same LAMA.

Methods

Randomized controlled open-label trial. Persons aged 30 years or older with a once-daily LAMA prescription and a confirmed COPD diagnosis were recruited. Participants were randomized in a 1:1 ratio to either morning or evening LAMA administration. Complete follow-up for the primary outcome, hospitalization-requiring AECOPD, or death from all causes within 1 year was captured from the Danish National Health Register, as were patient-reported outcome assessments at 6 and 12 months.

Results

A total of 10,013 participants were randomized, and the recruitment process started on 9 March 2023. Secondary outcomes include (i) moderate COPD exacerbations; (ii) all-cause hospitalization; (iii) ICU admission; (iv) need for non-invasive ventilation; and (v) all-cause mortality, among others. All outcomes will be evaluated 12 months after recruitment.

Clinical trial registration:ClinicalTrials.gov, NCT05563675.
Microbial metabolic potential of hydrothermal vent chimneys along the submarine ring of fire
Authors: Murray Laura , Fullerton Heather , Moyer Craig L.
Published: August 2024

Hydrothermal vents host a diverse community of microorganisms that utilize chemical gradients from the venting fluid for their metabolisms. The venting fluid can solidify to form chimney structures that these microbes adhere to and colonize. These chimney structures are found throughout many different locations in the world’s oceans. In this study, comparative metagenomic analyses of microbial communities on five chimney structures from around the Pacific Ocean were elucidated focusing on the core taxa and genes that are characteristic of each of these hydrothermal vent chimneys. The differences among the taxa and genes found at each chimney due to parameters such as physical characteristics, chemistry, and activity of the vents were highlighted. DNA from the chimneys was sequenced, assembled into contigs, and annotated for gene function. Genes used for carbon, oxygen, sulfur, nitrogen, iron, and arsenic metabolisms were found at varying abundances at each of the chimneys, largely from either Gammaproteobacteria or Campylobacteria. Many taxa shared an overlap of these functional metabolic genes, indicating that functional redundancy is critical for life at these hydrothermal vents. A high relative abundance of oxygen metabolism genes coupled with a low abundance of carbon fixation genes could be used as a unique identifier for inactive chimneys. Genes used for DNA repair, chemotaxis, and transposases were found at high abundances at each of these hydrothermal chimneys allowing for enhanced adaptations to the ever-changing chemical and physical conditions encountered.
Diversity of Shiga toxin transducing phages in Escherichia coli O145:H28 and the different Shiga toxin 2 production levels associated with short- or long-tailed phages
Authors: Nakamura Keiji , Taniguchi Itsuki , Gotoh Yasuhiro , Isobe Junko , Kimata Keiko , Igawa Yukiko , Kitahashi Tomoko , Takahashi Yohei , Nomoto Ryohei , Iwabuchi Kaori , Morimoto Yo , Iyoda Sunao , Hayashi Tetsuya
Published: August 2024

Shiga toxin (Stx)-producing Escherichia coli (STEC) causes serious gastrointestinal illness, including hemorrhagic colitis and hemolytic uremic syndrome. Two types of Stxs (Stx1 and Stx2) are known and both are encoded by bacteriophages (Stx phages), but the production of Stx2 is known to be a major risk factor for severe STEC infections. The production of Stx2, but not Stx1, is tightly coupled with the induction of Stx phages, and Stx2 production levels vary between STEC strains even within the same serotype. Here, we analyzed the genomic diversity of all Stx phages in 71 strains representing the entire O145:H28 lineage, one of the often highly pathogenic STECs, and the relationship between the variations in Stx phage genomes and the levels of Stx2 production by host strains. Our analysis reveals highly dynamic natures of Stx phages in O145:H28, including the independent acquisition of similar Stx phages by different sublineages, the recent transfer of Stx phage between different sublineages, and the frequent gain and loss of Stx phages in some sublineages. We also show the association of the Stx2 phage types with the Stx2 production levels of host strains: strains carrying short-tailed Stx2 phages exhibited significantly higher Stx2 production levels than those carrying long-tailed Stx2 phages. Detailed analyses of the Stx2 phage genomes revealed that both of short- and long-tailed phages exhibited sequence diversification and they were divided into two groups, respectively, based on the sequence similarity of the phage early region encoding genes responsible for phage induction, short-tailed phages contained early regions clearly different in genetic organization from those in long-tailed phages. Therefore, the variations in the early regions between short-and long-tailed Stx2 phages appeared to be linked to a striking difference in Stx2 production levels in their host strains. These results broaden our understanding of the diversification and dynamism of Stx phages in O145:H28 and the association of Stx2 phage types with the Stx2 production level in this STEC lineage.
Effectiveness of acupuncture in treating patients with pain and mental health concerns: the results of the Alberta Complementary Health Integration Project
Authors: Lu Mingshan , Sharmin Sumaiya , Tao Yong , Xia Xin , Yang Gongliang , Cong Yingying , Yang Guanhu , Jiang Jing , Xiao Yun , Peng Laura , Quan Joshua , Xu Bentong
Published: August 2024

Background

This study presents real-world evidence on the clinical outcomes of the Alberta Complementary Health Integration Project (ABCHIP), which utilized acupuncture to address pain and mental health issues in two vulnerable populations in Alberta: youth (aged 24 and below) and elderly (aged 55 and above).

Methods

Over 282 days, a total of 606 patients received 5,424 acupuncture treatments. Tailored to each patients’ specific pain and mental health concerns, an individualized treatment plan was selected, following a standard treatment protocol lasting 1 to 3 months. Patients were evaluated at least twice: initially and upon completing therapy. Primary treatment outcomes were assessed using various measures, including the Brief Pain Inventory (BPI), Pittsburgh Sleep Quality Index (PSQI), Patient Health Questionnaire 9 (PHQ9), PROMIS Anxiety 8a and its pediatric form PROMIS Anxiety-Pediatric, PROMIS Short Form v1.0 Fatigue 8a and its pediatric counterpart PROMIS Pediatric Short Form v2.0 Fatigue 10a, PROMIS Short Form v1.1 Anger 5a and its version PROMIS SF v2.0 5a, and EQ-5D-5L. These measures gauged pain reduction, improved sleep quality, reduced depression, anxiety, fatigue, anger, and quality of life, respectively.

Results

Analysis of data from 500 patients who received at least 6 acupuncture sessions through ABCHIP showed statistically significant improvements in clinical outcomes. Among this group, the subgroup of 235 patients who received at least 12 sessions demonstrated the most favorable treatment outcomes, including an 75.5% reduction in pain severity, a 53.1% improvement in sleep quality, a 78.4% drop in depression, a 41.1% decline in anxiety, a 43.7% decrease in fatigue, a 38.2% decrease in anger, and a 42.6% improvement in overall quality of life.

Conclusion

Integrating acupuncture with usual care demonstrates promise in enhancing mental health, alleviating chronic and general pain, and improving overall quality of life. The findings suggest that integrative programs, such as ABCHIP, present an effective approach to addressing pain and mental health concerns in vulnerable populations, providing valuable insights for future healthcare interventions.
Focused ultrasound therapy for Alzheimer’s disease: exploring the potential for targeted amyloid disaggregation
Authors: Scott Kurt , Klaus Stephen P.
Published: August 2024

Introduction

Alzheimer’s disease, a progressive neurodegenerative disorder, is marked by beta-amyloid plaque accumulation and cognitive decline. The limited efficacy and significant side effects of anti-amyloid monoclonal antibody therapies have prompted exploration into innovative treatments like focused ultrasound therapy. Focused ultrasound shows promise as a non-invasive technique for disrupting the blood–brain barrier, potentially enhancing drug delivery directly to the brain and improving the penetration of existing therapeutic agents.

Methods

This systematic review was conducted using PubMed and Embase databases, focusing on studies published in the last ten years that examined the use of low–intensity focused ultrasound for blood–brain barrier disruption in Alzheimer’s disease. The search strategy encompassed terms related to Alzheimer’s disease, focused ultrasound, and the blood–brain barrier. Studies were selected based on predefined inclusion and exclusion criteria. The quality of included studies was assessed using the Oxford Centre for Evidence-Based Medicine Levels of Evidence framework.

Results

Twelve studies were analyzed, the results of which suggested that low intensity focused ultrasound when combined with microbubbles may safely and transiently disrupt the blood–brain barrier. These studies, primarily early-phase and observational, highlight the potential feasibility of focused ultrasound in facilitating drug delivery to the brain for the treatment of Alzheimer’s disease. Notably, one study reported positive impacts on cognitive tests, suggesting potential direct therapeutic effects of focused ultrasound beyond blood–brain barrier disruption.

Conclusion

The results of the included studies indicate the use of focused ultrasound in Alzheimer’s disease treatment might be safe and effective in transiently opening the blood–brain barrier. Although current evidence is promising, further research is needed to establish generalizability. Future studies should also aim to further elucidate the mechanisms of action of low-intensity focused ultrasound as well as microbubbles for blood–brain barrier opening and explore potential clinical benefits beyond blood–brain barrier opening such as impacts on cognitive outcomes. Future studies should also aim for greater participant diversity to ensure findings are applicable across the full spectrum of Alzheimer’s disease patients.
Effects of post-acute COVID-19 syndrome on cerebral white matter and emotional health among non-hospitalized individuals
Authors: Churchill Nathan W. , Roudaia Eugenie , Chen J. Jean , Sekuler Allison , Gao Fuqiang , Masellis Mario , Lam Benjamin , Cheng Ivy , Heyn Chris , Black Sandra E. , MacIntosh Bradley J. , Graham Simon J. , Schweizer Tom A.
Published: August 2024

Introduction

Post-acute COVID syndrome (PACS) is a growing concern, given its impact on mental health and quality of life. However, its effects on cerebral white matter remain poorly understood, particularly in non-hospitalized cohorts. The goals of this cross-sectional, observational study were to examine (1) whether PACS was associated with distinct alterations in white matter microstructure, compared to symptom-matched non-COVID viral infection; and (2) whether microstructural alterations correlated with indices of post-COVID emotional health.

Methods

Data were collected for 54 symptomatic individuals who tested positive for COVID-19 (mean age 41 ± 12 yrs., 36 female) and 14 controls who tested negative for COVID-19 (mean age 41 ± 14 yrs., 8 female), with both groups assessed an average of 4–5 months after COVID testing. Diffusion magnetic resonance imaging data were collected, and emotional health was assessed via the NIH emotion toolbox, with summary scores indexing social satisfaction, well-being and negative affect.

Results

Despite similar symptoms, the COVID-19 group had reduced mean and axial diffusivity, along with increased mean kurtosis and neurite dispersion, in deep white matter. After adjusting for social satisfaction, higher levels of negative affect in the COVID-19 group were also correlated with increased mean kurtosis and reduced free water in white matter.

Discussion

These results provide preliminary evidence that indices of white matter microstructure distinguish PACS from symptomatic non-COVID infection. Moreover, white matter effects seen in PACS correlate with the severity of emotional sequelae, providing novel insights into this highly prevalent disorder.