The Role of Interelukin -6, Resistin and Cystatin C in Iraqi Patients with Chronic Kidney Disease
Authors:   Department of Optical Techniques , Al Noor University College, Ali Baraa , Mohammad Fatima ,   Department of Chemistry, College of Science, Mosul University
Published: August 2024

Abstract— Background: Studies have found that Chronic Kidney Disease (CKD) is fairly prevalent throughout the world. Due to their comorbidities and lifestyle, patients receiving hemodialysis for chronic renal illness are more prone to show changes in some biochemical indicators. The present work was conducted to study the role of some biochemical factors, including interleukin-6, resistin, and cystatin C. and the relationship between cystatin C and creatinine in order to track the progression of individuals with chronic renal disease. Objective: Due to the rise in instances of chronic kidney disease in Mosul city, the primary objective of the study was to examine levels of interelukin_6, Resistin , Cystatin C and some biochemical parameters in the blood of patients and compare their levels in healthy people, and to investigate the relationship of serum interelukin _6 with the development of the disease. Materials and methods: The study enrolled (80) cases of well diagnosed chronic kidney disease Iraqi patients, while the healthy group included (79) individuals. The age of both groups ranged between 18 and 55 years. Blood samples (5ml) have been used for serum separation , which is in turn used in the biochemical analysis, which included determination of biochemical parameters (interleukin-6, resistin, and cystatin C) using the protocol of ELISA kit, while estimated Cystatin C / Creatinine ratio . Results: The study's findings revealed that the levels of interleukin-6, resistin, cystatin C, and creatinine had increased significantly (P ≤ 0.01). The cystatin C/creatinine ratio was found to be significantly (P≤0.01) higher in the patient group as compared to the control group, A substantial significant at (P ≤ 0.01) decrease in albumin concentration was noted, whereas C- reactive protein concentration and the C-reactive protein/albumin ratio did not differ significantly at (P ≤ 0.05) between the patient group and the control group. A correlation study has been done between the parameters (interleukin-6, resistin and cystatin C) and other biochemical parameters. IL-6- shows a significant positive correlation with cystatin C and the cystatin C/ Creatinine ratio(r = 0.504, p = 0.02 and r = 0.5, p = 0.01 respectively), while non significant with other parameters ,there is a highly significant positive association between Resistin and creatinine as well as between Cystatin C /creatinine ratio in the group of patients with CKD (r= 0.305, p=0.01, and r= 0.30, p=0.05, respectively). On other hand there was non significant correlation with other parameters. The results revealed the presence of significant positive correlation of Cystatin C associated with IL-6- and Cystatin C / Creatinine ratio ( r= 0.504, p=0.02 and r= 0.63,p=0.00 respectivly ), There was non significant correlation between ( IL- 6- ,Resistin, Cystatin C) and other paramerters . Conclusion: A high concentration of IL-6, Resistin, and Cystatin C is associated with chronic kidney disease. The development of the disease is tightly correlated with the studied biochemical characteristics.