bluuebunny/crossref_metadata_2025 huggingface dataset:
Phosphorylation of H3-Thr3 by Haspin Is Required for Primary Cilia Regulation
Authors: Quadri Roberto , Sertic Sarah , Ghilardi Anna , Rondelli Diego , Gallo Guido Roberto , Del Giacco Luca , Muzi-Falconi Marco
Published: July 2021

Primary cilia are commonly found on most quiescent, terminally differentiated cells and play a major role in the regulation of the cell cycle, cell motility, sensing, and cell–cell communication. Alterations in ciliogenesis and cilia maintenance are causative of several human diseases, collectively known as ciliopathies. A key determinant of primary cilia is the histone deacetylase HDAC6, which regulates their length and resorption and whose distribution is regulated by the death inducer-obliterator 3 (Dido3). Here, we report that the atypical protein kinase Haspin is a key regulator of cilia dynamics. Cells defective in Haspin activity exhibit longer primary cilia and a strong delay in cilia resorption upon cell cycle reentry. We show that Haspin is active in quiescent cells, where it phosphorylates threonine 3 of histone H3, a known mitotic Haspin substrate. Forcing Dido3 detachment from the chromatin prevents Haspin inhibition from impacting cilia dynamics, suggesting that Haspin activity is required for the relocalization of Dido3–HDAC6 to the basal body. Exploiting the zebrafish model, we confirmed the physiological relevance of this mechanism. Our observations shed light on a novel player, Haspin, in the mechanisms that govern the determination of cilia length and the homeostasis of mature cilia.
Microbeam Radiotherapy—A Novel Therapeutic Approach to Overcome Radioresistance and Enhance Anti-Tumour Response in Melanoma
Authors: Trappetti Verdiana , Fazzari Jennifer M. , Fernandez-Palomo Cristian , Scheidegger Maximilian , Volarevic Vladislav , Martin Olga A. , Djonov Valentin G.
Published: July 2021

Melanoma is the deadliest type of skin cancer, due to its invasiveness and limited treatment efficacy. The main therapy for primary melanoma and solitary organ metastases is wide excision. Adjuvant therapy, such as chemotherapy and targeted therapies are mainly used for disseminated disease. Radiotherapy (RT) is a powerful treatment option used in more than 50% of cancer patients, however, conventional RT alone is unable to eradicate melanoma. Its general radioresistance is attributed to overexpression of repair genes in combination with cascades of biochemical repair mechanisms. A novel sophisticated technique based on synchrotron-generated, spatially fractionated RT, called Microbeam Radiation Therapy (MRT), has been shown to overcome these treatment limitations by allowing increased dose delivery. With MRT, a collimator subdivides the homogeneous radiation field into an array of co-planar, high-dose microbeams that are tens of micrometres wide and spaced a few hundred micrometres apart. Different preclinical models demonstrated that MRT has the potential to completely ablate tumours, or significantly improve tumour control while dramatically reducing normal tissue toxicity. Here, we discuss the role of conventional RT-induced immunity and the potential for MRT to enhance local and systemic anti-tumour immune responses. Comparative gene expression analysis from preclinical tumour models indicated a specific gene signature for an ‘MRT-induced immune effect’. This focused review highlights the potential of MRT to overcome the inherent radioresistance of melanoma which could be further enhanced for future clinical use with combined treatment strategies, in particular, immunotherapy.
Effect of ZnSO4, MnSO4 and FeSO4 on the Partial Hydrogenation of Benzene over Nano Ru-Based Catalysts
Authors: Sun Haijie , Fan Yiru , Sun Xiangrong , Chen Zhihao , Li Huiji , Peng Zhikun , Liu Zhongyi
Published: July 2021

Nano Ru-based catalysts, including monometallic Ru and Ru-Zn nanoparticles, were synthesized via a precipitation method. The prepared catalysts were evaluated on partial hydrogenation of benzene towards cyclohexene generation, during which the effect of reaction modifiers, i.e., ZnSO4, MnSO4, and FeSO4, was investigated. The fresh and the spent catalysts were thoroughly characterized by XRD, TEM, SEM, XPS, XRF, and DFT studies. It was found that Zn2+ or Fe2+ could be adsorbed on the surface of a monometallic Ru catalyst, where a stabilized complex could be formed between the cations and the cyclohexene. This led to an enhancement of catalytic selectivity towards cyclohexene. Furthermore, electron transfer was observed from Zn2+ or Fe2+ to Ru, hindering the catalytic activity towards benzene hydrogenation. In comparison, very few Mn2+ cations were adsorbed on the Ru surface, for which no cyclohexene could be detected. On the other hand, for Ru-Zn catalyst, Zn existed as rodlike ZnO. The added ZnSO4 and FeSO4 could react with ZnO to generate (Zn(OH)2)5(ZnSO4)(H2O) and basic Fe sulfate, respectively. This further benefited the adsorption of Zn2+ or Fe2+, leading to the decrease of catalytic activity towards benzene conversion and the increase of selectivity towards cyclohexene synthesis. When 0.57 mol·L−1 of ZnSO4 was applied, the highest cyclohexene yield of 62.6% was achieved. When MnSO4 was used as a reaction modifier, H2SO4 could be generated in the slurry via its hydrolysis, which reacted with ZnO to form ZnSO4. The selectivity towards cyclohexene formation was then improved by the adsorbed Zn2+.
Regulation of VEGFR Signalling in Lymphatic Vascular Development and Disease: An Update
Authors: Secker Genevieve A. , Harvey Natasha L.
Published: July 2021

The importance of lymphatic vessels in a myriad of human diseases is rapidly gaining recognition; lymphatic vessel dysfunction is a feature of disorders including congenital lymphatic anomalies, primary lymphoedema and obesity, while improved lymphatic vessel function increases the efficacy of immunotherapy for cancer and neurological disease and promotes cardiac repair following myocardial infarction. Understanding how the growth and function of lymphatic vessels is precisely regulated therefore stands to inform the development of novel therapeutics applicable to a wide range of human diseases. Lymphatic vascular development is initiated during embryogenesis following establishment of the major blood vessels and the onset of blood flow. Lymphatic endothelial progenitor cells arise from a combination of venous and non-venous sources to generate the initial lymphatic vascular structures in the vertebrate embryo, which are then further ramified and remodelled to elaborate an extensive lymphatic vascular network. Signalling mediated via vascular endothelial growth factor (VEGF) family members and vascular endothelial growth factor receptor (VEGFR) tyrosine kinases is crucial for development of both the blood and lymphatic vascular networks, though distinct components are utilised to different degrees in each vascular compartment. Although much is known about the regulation of VEGFA/VEGFR2 signalling in the blood vasculature, less is understood regarding the mechanisms by which VEGFC/VEGFD/VEGFR3 signalling is regulated during lymphatic vascular development. This review will focus on recent advances in our understanding of the cellular and molecular mechanisms regulating VEGFA-, VEGFC- and VEGFD-mediated signalling via VEGFRs which are important for driving the construction of lymphatic vessels during development and disease.
Newborn Screening for SCID: Experience in Spain (Catalonia)
Authors: Argudo-Ramírez Ana , Martín-Nalda Andrea , González de Aledo-Castillo Jose Manuel , López-Galera Rosa , Marín-Soria Jose Luis , Pajares-García Sonia , Martínez-Gallo Mónica , García-Prat Marina , Colobran Roger , Riviere Jacques G. , Quintero Yania , Collado Tatiana , Ribes Antonia , García-Villoria Judit , Soler-Palacín Pere
Published: July 2021

Newborn screening (NBS) for severe combined immunodeficiency (SCID) started in Catalonia in January-2017, being the first Spanish and European region to universally include this testing. In Spain, a pilot study with 5000 samples was carried out in Seville in 2014; also, a research project with about 35,000 newborns will be carried out in 2021–2022 in the NBS laboratory of Eastern Andalusia. At present, the inclusion of SCID is being evaluated in Spain. The results obtained in the first three and a half years of experience in Catalonia are presented here. All babies born between January-2017 and June-2020 were screened through TREC-quantification in DBS with the Enlite Neonatal TREC-kit from PerkinElmer. A total of 222,857 newborns were screened, of which 48 tested positive. During the study period, three patients were diagnosed with SCID: an incidence of 1 in 74,187 newborns; 17 patients had clinically significant T-cell lymphopenia (non-SCID) with an incidence of 1 in 13,109 newborns who also benefited from the NBS program. The results obtained provide further evidence of the benefits of early diagnosis and curative treatment to justify the inclusion of this disease in NBS programs. A national NBS program is needed, also to define the exact SCID incidence in Spain.
The Effect of Mipomersen in the Management of Patients with Familial Hypercholesterolemia: A Systematic Review and Meta-Analysis of Clinical Trials
Authors: Astaneh Behrooz , Makhdami Nima , Astaneh Vala , Guyatt Gordon
Published: July 2021

Background: Familial hypercholesterolemia (FH) lead to significant adverse effects in coronary arteries. Mipomersen is a second-generation antisense oligonucleotide that inhibits the synthesis of apolipoprotein B-100, an essential component of low density lipoprotein (LDL), and thus decreases the production of LDL. We aimed to determine the effect of mipomersen in patients with FH. Methods: We searched Ovid Medline, Ovid EMBASE, WHO ICTRP search portal, ISI database, the reference lists of relevant articles, and also Google Scholar to retrieve articles. All randomized controlled trials (RCTs) comparing patients with FH receiving mipomersen as an add-on and a parallel group receiving a placebo or no intervention were selected. Results: Five studies with more than 500 patients were included. All had low risk of bias. Pooling data showed that mipomersen probably reduces LDL compared with placebo [mean difference: −24.79, 95% CI (−30.15, −19.43)] but with a moderate level of certainty. There was a high level of evidence for injection site reactions [RR = 2.56, CI (1.47–4.44)] and a low level for increased serum alanine transaminase (ALT) > 3 times upper limit of normal (ULN) [RR = 5.19, CI (1.01–26.69)]. Conclusion: A moderate level of evidence in decreasing serum LDL indicates that we are uncertain if this drug provides benefit in any outcome important to patients. Although a low level of evidence for an increase in serum ALT leaves uncertainty about this adverse effect, injection site reactions in 10% or more of patients can be an important concern.
The Level of Conus Medullaris in 629 Healthy Japanese Individuals
Authors: Nakashima Hiroaki , Ito Keigo , Katayama Yoshito , Tsushima Mikito , Ando Kei , Kobayashi Kazuyoshi , Machino Masaaki , Ito Sadayuki , Koshimizu Hiroyuki , Segi Naoki , Tomita Hiroyuki , Imagama Shiro
Published: July 2021

The conus medullaris typically terminates at the L1 level; however, variations in its level and the factors associated with the conus medullaris level are unclear. We investigated the level of conus medullaris on magnetic resonance imaging in healthy volunteers. In total, 629 healthy adult volunteers (≥50 individuals of each sex and in each decade of age from 20 to 70) were enrolled. The level of the conus medullaris was assessed based on the T2-weighted sagittal magnetic resonance images, and factors affecting its level were investigated employing multivariate regression analysis including the participants’ background and radiographical parameters. L1 was the most common conus medullaris level. Participant height was significantly shorter in the caudally placed conus medullaris (p = 0.013). With respect to the radiographical parameters, pelvic incidence (p = 0.003), and pelvic tilt (p = 0.03) were significantly smaller in participants with a caudally placed conus medullaris. Multiple regression analysis showed that the pelvic incidence (p < 0.0001) and height (p < 0.0001) were significant factors affecting the conus medullaris level. These results indicated that the length of the spinal cord varies little among individuals and that skeletal differences affect the level of the conus medullaris.
Immunomodulatory Nanomedicine for the Treatment of Atherosclerosis
Authors: Peters Linsey J. F. , Jans Alexander , Bartneck Matthias , van der Vorst Emiel P. C.
Published: July 2021

Atherosclerosis is the main underlying cause of cardiovascular diseases (CVDs), which remain the number one contributor to mortality worldwide. Although current therapies can slow down disease progression, no treatment is available that can fully cure or reverse atherosclerosis. Nanomedicine, which is the application of nanotechnology in medicine, is an emerging field in the treatment of many pathologies, including CVDs. It enables the production of drugs that interact with cellular receptors, and allows for controlling cellular processes after entering these cells. Nanomedicine aims to repair, control and monitor biological and physiological systems via nanoparticles (NPs), which have been shown to be efficient drug carriers. In this review we will, after a general introduction, highlight the advantages and limitations of the use of such nano-based medicine, the potential applications and targeting strategies via NPs. For example, we will provide a detailed discussion on NPs that can target relevant cellular receptors, such as integrins, or cellular processes related to atherogenesis, such as vascular smooth muscle cell proliferation. Furthermore, we will underline the (ongoing) clinical trials focusing on NPs in CVDs, which might bring new insights into this research field.
Value of Transnasal Esophagoscopy in the Workup of Laryngo-Pharyngeal Reflux
Authors: Horvath Lukas , Fostiropoulos Karolos , Burri Emanuel , Kraft Marcel
Published: July 2021

Background: Laryngopharyngeal reflux (LPR) can display a variety of symptoms, and upper endoscopy is occasionally used for its investigation. The aim of the present study was to determine the value of transnasal esophagoscopy (TNE) in the workup of LPR. Methods: In 200 consecutive patients with suspected LPR, reflux symptom index (RSI), reflux finding score (RFS), oropharyngeal pH-monitoring (PHM) and transnasal esophagoscopy (TNE) were carried out and rated according to the Horvath Score. Results: In the investigation of LPR, TNE showed a sensitivity, specificity and accuracy of 96%, 85% and 95%, respectively. The most common pathologic TNE findings in LPR patients were an insufficient cardia, hiatal hernia, lymphoid follicles and visible reflux. Conclusions: TNE is a supportive method in the workup of LPR, which can display the underlying pathology and directly affect therapeutic decisions.
Concomitant Acute Ischemic Stroke and Upper Extremity Arterial Occlusion: Feasibility of Mechanical Thrombectomy of the Upper Limb Using Neurointerventional Devices and Techniques
Authors: Vollherbst Dominik F. , Ulfert Christian , Maus Volker , Boujan Timan , Henkes Hans , Bendszus Martin , Möhlenbruch Markus A.
Published: July 2021

Background: Concomitant acute ischemic stroke (AIS) caused by large vessel occlusion (LVO), and acute upper extremity arterial occlusion causing upper limb ischemia (ULI) is a rarely observed coincidence. The first-line treatment for AIS is mechanical thrombectomy (MT), with or without additional intravenous thrombolysis, while there are different pharmacological, surgical and endovascular treatment options for an acute occlusion of the UL arteries. Here, we describe the practicability, efficacy and safety of neurointerventional devices and techniques for MT of upper extremity arterial occlusions. Materials and Methods: A retrospective analysis of prospectively collected patient databases from four neurovascular centers was performed. Clinical and imaging data, as well as procedural parameters, were assessed. Results: Seven out of 6138 patients (incidence: 0.11%) presenting with an AIS due to the occlusion of craniocervical arteries requiring MT and a concomitant occlusion of the brachial (4/7), axillary (2/7), or ulnar (1/7) artery causing acute ULI were identified. Craniocervical MT was technically successful in all cases. Subsequent MT of the upper limb was performed using neurointerventional thrombectomy techniques, most frequently stent retriever thrombectomy (in 4/7 cases) and direct aspiration (in 7/7 cases). MT achieved successful recanalization in 6/7 cases, and the UL completely recovered in all six cases. In one case, recanalization was not successful, and the patient still had a marginally threatened extremity after the procedure, which improved after pharmacological therapy. Conclusion: In the rare case of AIS requiring MT and concomitant acute upper extremity arterial occlusion, MT of the UL arteries using neurointerventional devices and techniques is practical, effective, and safe.
Mental Health and Psychological Wellbeing during the COVID-19 Lockdown: A Longitudinal Study in the Balearic Islands (Spain)
Authors: Ripoll Joana , Contreras-Martos Sara , Esteva Magdalena , Soler Aina , Serrano-Ripoll Maria Jesús
Published: July 2021

Confining the entire population to a lockdown after the outbreak of SARS-CoV-2 was an unprecedented measure designed to protect the health of those living in Spain. The objective of the present study is to assess the evolution of mental health and psychological wellbeing during lockdown. To do this, we carried out a longitudinal study, via an online survey over the eight weeks of lockdown (weekly assessments). Sociodemographic variables were recorded, along with data related to COVID-19, psychological wellbeing (anxiety, depression, psychotropic drugs, consultations made to improve mood or anxiety), life satisfaction, and self-perceived health. A total of 681 individuals participated in the study, 76.8% were women; the mean age was 43 years old (SD = 12.7). Initially, high scores were reported for anxiety, depression, and the number of consultations to improve mood, but these decreased significantly over the study period. The reverse seems to be true for life satisfaction, perceived good health, and intake of psychotropic drugs. We also identified groups whose psychological wellbeing was more susceptible to the effects of lockdown. Women, those worried about their jobs after the pandemic, and those afraid of being infected were the most affected individuals. More generally, after the initial negative effect on psychological wellbeing, various indicators improved over the lockdown period.
Prenatal and Postnatal Anxiety and Depression in Mothers during the COVID-19 Pandemic
Authors: Baran Joanna , Leszczak Justyna , Baran Rafał , Biesiadecka Anna , Weres Aneta , Czenczek-Lewandowska Ewelina , Kalandyk-Osinko Katarzyna
Published: July 2021

The aim of this study was to assess the changes in the occurrence of prenatal and postnatal anxiety and depression symptoms, and to assess what factors significantly affect the appearance of symptoms of depression and anxiety in young mothers. The study group consisted of 130 women after childbirth. Due to the ongoing restrictions caused by the COVID-19 pandemic, the survey was prepared online. The questionnaire was fully anonymous, and it contained the authors’ own questions and two standardized questionnaires: the Edinburgh Postnatal Depression Scale (EPDS) and Generalized Anxiety Disorders GAD-7. The conducted analysis clearly indicated that the level of postpartum depression, in as many as 52 of the mothers, had increased significantly compared to the time before delivery, when symptoms of depression were shown by 22 women (p = 0.009). However, there was no statistically significant change between prenatal and postnatal anxiety. There are many factors associated with postnatal depression. The strongest predictors turned out to be average socioeconomic status, history of anxiety disorders, past neurosis or depression, lack of or inadequate level of assistance from healthcare professionals, as well as lactation problems and postpartum pain.
Vanadium Dioxide–Iridium Composite Development: Specific Near Infrared Surface Plasmon Resonance
Authors: Bercea Adrian Ionut , Champeaux Corinne , Constantinescu Catalin Daniel , Dumas-Bouchiat Frédéric
Published: July 2021

This work serves as a roadmap for the development of a Vanadium dioxide (VO2)–Iridium composite based on the self-assembly of closely packed colloidal polystyrene microspheres (P-spheres) coupled with a Pulsed Laser Deposition (PLD) process. The self-assembly of a monolayer of PS is performed on an Al2O3-c substrate, using an adapted Langmuir–Blodgett (LB) process. Then, on the substrate covered with P-spheres, a 50-nanometer Iridium layer is deposited by PLD. The Iridium deposition is followed by the removal of PS with acetone, revealing an array of triangular shaped metallic elements formed on the underlaying substrate. In a last deposition step, 50-, 100- and 200-nanometer thin films of VO2 are deposited by PLD on top of the substrates covered with the Iridium quasi-triangles, forming a composite. Adapting the size of the P-spheres leads to control of both the size of the Iridium micro-triangle and, consequently, the optical transmittance of the composite. Owing to their shape and size the Iridium micro-triangles exhibit localized surface plasmon resonance (LSPR) characterized by a selective absorption of light. Due to the temperature dependent properties of VO2, the LSPR properties of the composite can be changeable and tunable.
Statistics of Simulated Storm Waves over Bathymetry
Authors: Latifah Arnida Lailatul , Handri Durra , Shabrina Ayu , Hariyanto Henokh , van Groesen E.
Published: July 2021

This paper shows simulations of high waves over different bathymetries to collect statistical information, particularly kurtosis and crest exceedance, that quantifies the occurrence of exceptionally extreme waves. This knowledge is especially pertinent for the design and operation of marine structures, safe ship trafficking, and mooring strategies for ships near the coast. Taking advantage of the flexibility to perform numerical simulations with HAWASSI software, with the aim of investigating the physical and statistical properties for these cases, this paper investigates the change in wave statistics related to changes in depth, breaking and differences between long- and short-crested waves. Three different types of bathymetry are considered: run-up to the coast with slope 1/20, waves over a shoal, and deep open-water waves. Simulations show good agreement in the examined cases compared with the available experimental data and simulations. Then predictive simulations for cases with a higher significant wave height illustrate the changes that may occur during storm events.
Effectiveness of Floating Breakwater in Special Configurations for Protecting Nearshore Infrastructures
Authors: Zhang Chi , Magee Allan R.
Published: July 2021

This paper investigates the effectiveness of floating breakwaters consisting of two barges (L-shape), three barges (U-shape), four barges (barge frame), and conventional single floating breakwater. The floating breakwaters of different spatial layouts have sheltered internal gaps/moonpools when compared to their conventional counterparts. The motions of these floating breakwaters and their effectiveness in wave transmission and motion reduction of the protected floating bodies are evaluated. The study is conducted based on a subsystem of a floating hydrocarbon storage facility that combines a floating breakwater with two floating tanks, studied previously. Numerical models based on linear potential flow theory are built for these floating breakwaters with and without the floating tanks. The numerical models of the barge frame are validated through laboratory experiments, and the dipole damping lids to reduce the resonant fluid motions in the gaps/moonpools are calibrated with experimental results. The L-shape floating breakwater is found effective in reducing wave transmissions without the presence of the floating tanks, while the barge frame is the most effective in the motion reduction of the floating tanks. In addition, significant fluid resonant motions are identified for all investigated floating breakwaters, including the conventional one. Orienting the structure obliquely can improve the performance of the floating breakwaters of special configurations, especially the barge frame. The results and findings show the importance of considering the protected floating structures in the analysis when designing floating breakwaters for many types of marine structures, including floating fish farms and floating cities in coastal waters.
Semi-Natural Areas on Post-Mining Brownfields as an Opportunity to Strengthen the Attractiveness of a Small Town. An Example of Radzionków in Southern Poland
Authors: Kantor-Pietraga Iwona , Zdyrko Aleksandra , Bednarczyk Jakub
Published: July 2021

The socio-economic and political changes of the end of the 20th century in Central and Eastern Europe had significant impacts on the transformation of urban spaces, especially in industrial and mining towns. The article attempts to explain the essence of these changes concerning the spatial development of—a small post-mining town in southern Poland. This article evaluates urban development policy in response to the significant land-use changes in the small post-mining city of Radzionków, with particular attention to the transformation of brownfield sites to semi-natural areas of regional importance. This issue is interesting for two reasons. First, this small city, located in a large European agglomeration, has to face competition focused on interesting regional projects. Second, there is a desire for reindustrialization as a remedy for job losses in mining and heavy industry. The successful establishment of a large botanical garden in this city provides a case study for discussing the future of small post-industrial cities and the development of land use policy regarding valuable natural areas located in post-industrial and post-mining areas. This study also indicates the vital role of the creative management factor.
Sources of and Control Measures for PTE Pollution in Soil at the Urban Fringe in Weinan, China
Authors: Han Lei , Chen Rui , Liu Zhao , Chang Shanshan , Zhao Yonghua , Li Leshi , Li Risheng , Xia Longfei
Published: July 2021

The environment of the urban fringe is complex and frangible. With the acceleration of industrialization and urbanization, the urban fringe has become the primary space for urban expansion, and the intense human activities create a high risk of potentially toxic element (PTE) pollution in the soil. In this study, 138 surface soil samples were collected from a region undergoing rapid urbanization and construction—Weinan, China. Concentrations of As, Pb, Cr, Cu, and Ni (Inductively Coupled Plasma Mass Spectrometry, ICP-MS) and Hg (Atomic Fluorescence Spectrometry, AFS) were measured. The Kriging interpolation method was used to create a visualization of the spatial distribution characteristics and to analyze the pollution sources of PTEs in the soil. The pollution status of PTEs in the soil was evaluated using the national environmental quality standards for soils in different types of land use. The results show that the content range of As fluctuated a small amount and the coefficient of variation is small and mainly comes from natural soil formation. The content of Cr, Cu, and Ni around the automobile repair factory, the prefabrication factory, and the building material factory increased due to the deposition of wear particles in the soil. A total of 13.99% of the land in the study area had Hg pollution, which was mainly distributed on category 1 development land and farmland. Chemical plants were the main pollution sources. The study area should strictly control the industrial pollution emissions, regulate the agricultural production, adjust the land use planning, and reduce the impact of pollution on human beings. Furthermore, we make targeted remediation suggestions for each specific land use type. These results are of theoretical significance, will be of practical value for the control of PTEs in soil, and will provide ecological environmental protection in the urban fringe throughout the urbanization process.
In Vitro Assays for the Assessment of Impaired Mitochondrial Bioenergetics in Equine Atypical Myopathy
Authors: Kruse Caroline-J. , Stern David , Mouithys-Mickalad Ange , Niesten Ariane , Art Tatiana , Lemieux Hélène , Votion Dominique-M.
Published: July 2021

Equine atypical myopathy is a seasonal intoxication of grazing equids. In Europe, this poisoning is associated with the ingestion of toxins contained in the seeds and seedlings of the sycamore maple (Acer pseudoplatanus). The toxins involved in atypical myopathy are known to inhibit ß-oxidation of fatty acids and induce a general decrease in mitochondrial respiration, as determined by high-resolution respirometry applied to muscle samples taken from cases of atypical myopathy. The severe impairment of mitochondrial bioenergetics induced by the toxins may explain the high rate of mortality observed: about 74% of horses with atypical myopathy die, most within the first two days of signs of poisoning. The mechanism of toxicity is not completely elucidated yet. To improve our understanding of the pathological process and to assess therapeutic candidates, we designed in vitro assays using equine skeletal myoblasts cultured from muscle biopsies and subjected to toxins involved in atypical myopathy. We established that equine primary myoblasts do respond to one of the toxins incriminated in the disease.
Loss of Group II Metabotropic Glutamate Receptor Signaling Exacerbates Hypertension in Spontaneously Hypertensive Rats
Authors: Hsu Julia Chu-Ning , Sekizawa Shinichi , Tochinai Ryota , Kuwahara Masayoshi
Published: July 2021

High blood pressure is a major risk factor of cerebro-cardiovascular outcomes. Blood pressure is partly regulated by the autonomic nervous system and its reflex functions; therefore, we hypothesized that pharmacological intervention in the brainstem that can regulate blood pressure could be a novel therapeutic strategy to control hypertension. We infused a group II metabotropic glutamate receptor (mGluR) antagonist (LY341495, 0.40 μg/day), using a mini-osmotic pump, into the dorsal medulla oblongata in young spontaneously hypertensive rats (SHRs), as this area is adjacent to the nucleus tractus solitarius (NTS), of which the neurons are involved in baroreflex pathways with glutamatergic transmission. Blood pressure was recorded for conscious rats with the tail cuff method. A 6-week antagonist treatment from 6 to 12 weeks of age slightly but significantly increased systolic blood pressure by >30 mmHg, compared to that in SHRs without treatment. Moreover, the effect continued even 3 weeks after the treatment ended, and concurred with an increase in blood catecholamine concentration. However, heart rate variability analysis revealed that LY341495 treatment had little effect on autonomic activity. Meanwhile, mRNA expression level of mGluR subtype 2, but not subtype 3 in the brainstem was significantly enhanced by the antagonist treatment in SHRs, possibly compensating the lack of mGluR signaling. In conclusion, mGluR2 signaling in the dorsal brainstem is crucial for preventing the worsening of hypertension over a relatively long period in SHRs, through a mechanism of catecholamine secretion. This may be a specific drug target for hypertension therapy.
Sensitivity of Ultrasonic Coda Wave Interferometry to Material Damage—Observations from a Virtual Concrete Lab
Authors: Finger Claudia , Saydak Leslie , Vu Giao , Timothy Jithender J. , Meschke Günther , Saenger Erik H.
Published: July 2021

Ultrasonic measurements are used in civil engineering for structural health monitoring of concrete infrastructures. The late portion of the ultrasonic wavefield, the coda, is sensitive to small changes in the elastic moduli of the material. Coda Wave Interferometry (CWI) correlates these small changes in the coda with the wavefield recorded in intact, or unperturbed, concrete specimen to reveal the amount of velocity change that occurred. CWI has the potential to detect localized damages and global velocity reductions alike. In this study, the sensitivity of CWI to different types of concrete mesostructures and their damage levels is investigated numerically. Realistic numerical concrete models of concrete specimen are generated, and damage evolution is simulated using the discrete element method. In the virtual concrete lab, the simulated ultrasonic wavefield is propagated from one transducer using a realistic source signal and recorded at a second transducer. Different damage scenarios reveal a different slope in the decorrelation of waveforms with the observed reduction in velocities in the material. Finally, the impact and possible generalizations of the findings are discussed, and recommendations are given for a potential application of CWI in concrete at structural scale.
Microstructure and Optical Properties of Nanostructural Thin Films Fabricated through Oxidation of Au–Sn Intermetallic Compounds
Authors: Skowronski Lukasz , Trzcinski Marek , Olszewska Aleksandra , Szczesny Robert
Published: July 2021

AuSn and AuSn2 thin films (5 nm) were used as precursors during the formation of semiconducting metal oxide nanostructures on a silicon substrate. The nanoparticles were produced in the processes of annealing and oxidation of gold–tin intermetallic compounds under ultra-high vacuum conditions. The formation process and morphology of a mixture of SnO2 and Au@SnOx (the core–shell structure) nanoparticles or Au nanocrystalites were carefully examined by means of spectroscopic ellipsometry (SE), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) combined with energy-dispersive X-ray spectroscopy (EDX). The annealing and oxidation of the thin film of the AuSn intermetallic compound led to the formation of uniformly distributed structures with a size of ∼20–30 nm. All of the synthesized nanoparticles exhibited a strong absorption band at 520–530 nm, which is typical for pure metallic or metal oxide systems.
Experimental Study on Mechanical Properties of High-Ductility Concrete against Combined Sulfate Attack and Dry–Wet Cycles
Authors: Li Lingling , Shi Junping , Kou Jialiang
Published: July 2021

Concrete will deteriorate and damage under sulfate attack.In order to study the degradation characteristics of HDC under sulfate attack, the mechanical properties of high-ductility concrete (HDC) were investigated using the uniaxial compressive strength test of HDC specimens soaked in different concentrations of sulfate solution and subjected to different times of dry–wet cycles. The variations in the compressive strength, loss rate of compressive strength, and the max compressive strength under the action of sulfate attack and dry–wet cycles were analyzed. The analytical expressions of damage variables were given. SEM was used to observe the microstructure of the sample, and the microdamage mechanism of the HDC was explored. The deterioration of the HDC was found to be the result of the combined action of sulfate attack and dry–wet cycles and was caused by physical attack and chemical attack. PVA prevented the rapid development of deterioration. On the basis of the change of compressive strength, the damage variable was established to quantitatively describe the degree of damage to HDC. The experimental results showed that with the increase in the number of dry–wet cycles, the compressive strength of HDC generally increased first and then decreased. As the concentration of the sulfate solution increased, the loss rate of the compressive strength of HDC generally increased and the max compressive strength gradually decreased. With the increase inthe number of dry–wet cycles, HDC first showed self-compacting characteristics and then gradually became destroyed. Compared with ordinary concrete (OC), HDC is superior to OC in sulfate resistance and dry–wet cycles. This study provided a test basis for the engineering application of HDC in sulfate attack and dry–wet cycles environment.
Effects of Application of Recycled Chicken Manure and Spent Mushroom Substrate on Organic Matter, Acidity, and Hydraulic Properties of Sandy Soils
Authors: Lipiec Jerzy , Usowicz Bogusław , Kłopotek Jerzy , Turski Marcin , Frąc Magdalena
Published: July 2021

Soil organic matter is a key resource base for agriculture. However, its content in cultivated soils is low and often decreases. This study aimed at examining the effects of long-term application of chicken manure (CM) and spent mushroom substrate (SMS) on organic matter accumulation, acidity, and hydraulic properties of soil. Two podzol soils with sandy texture in Podlasie Region (Poland) were enriched with recycled CM (10 Mg ha−1) and SMS (20 Mg ha−1), respectively, every 1–2 years for 20 years. The application of CM and SMS increased soil organic matter content at the depths of 0–20, 20–40, and 40–60 cm, especially at 0–20 cm (by 102–201%). The initial soil pH increased in the CM- and SMS-amended soil by 1.7–2.0 units and 1.0–1.2 units, respectively. Soil bulk density at comparable depths increased and decreased following the addition of CM and SMS, respectively. The addition of CM increased field water capacity (at –100 hPa) in the range from 45.8 to 117.8% depending on the depth within the 0–60 cm layer. In the case of the SMS addition, the value of the parameter was in the range of 42.4–48.5% at two depths within 0–40 cm. Depending on the depth, CM reduced the content of transmission pores (>50 µm) in the range from 46.3 to 82.3% and increased the level of residual pores (<0.5 µm) by 91.0–198.6%. SMS increased the content of residual pores at the successive depths by 121.8, 251.0, and 30.3% and decreased or increased the content of transmission and storage pores. Additionally, it significantly reduced the saturated hydraulic conductivity at two depths within 0–40 cm. The fitted unsaturated hydraulic conductivity at two depths within the 0–40 cm layer increased and decreased in the CM- and SMS-amended soils, respectively. The results provide a novel insight into the application of recycled organic materials to sequester soil organic matter and improve crop productivity by increasing soil water retention capacity and decreasing acidity. This is of particular importance in the case of the studied low-productivity sandy acidic soils that have to be used in agriculture due to limited global land resources and rising food demand.
Wear and Corrosion Resistance of Plasma Electrolytic Oxidation Coatings on 6061 Al Alloy in Electrolytes with Aluminate and Phosphate
Authors: Peng Zhenjun , Xu Hui , Liu Siqin , Qi Yuming , Liang Jun
Published: July 2021

Phosphate and aluminate electrolytes were used to prepare plasma electrolytic oxidation (PEO) coatings on 6061 aluminum alloy. The surface and cross-section microstructure, element distribution, and phase composition of the PEO coatings were characterized by SEM, EDS, XPS, and XRD. The friction and wear properties were evaluated by pin-on-disk sliding tests under dry conditions. The corrosion resistance of PEO coatings was investigated by electrochemical corrosion and salt spray tests in acidic environments. It was found that the PEO coatings prepared from both phosphate and aluminate electrolytes were mainly composed of α-Al2O3 and γ-Al2O3. The results demonstrate that a bi-layer coating is formed in the phosphate electrolyte, and a single-layered dense alumina coating with a hardness of 1300 HV is realizable in the aluminate electrolyte. The aluminate PEO coating had a lower wear rate than the phosphate PEO coating. However, the phosphate PEO coating showed a better corrosion resistance in acidic environment, which is mainly attributed to the presence of an amorphous P element at the substrate/coating interface.
Analysis of the Surface Condition and Changes in Crystallographic Structure of Zirconium Oxide Affected by Mechanical Processing
Authors: Regulska Kinga , Januszewicz Bartłomiej , Klimek Leszek , Palatyńska-Ulatowska Aleksandra
Published: July 2021

Zirconium oxide is a material commonly used in dental prosthetics for making cups of permanent prosthetic restorations. In order to properly prepare the surface of zirconium oxide for prosthetic treatment, it must be veneered with ceramics. The quality of cup-veneered ceramics is dependent on many factors, including the surface free energy (SFE) and transformation of zirconium oxide. The aim of the study was to investigate the type of phase transition and the value of free energy of the surface subjected to machining (wet and dry grinding, polishing). Quantitative and qualitative phase identification measurements showed that mechanical treatment causes transformation of the tetragonal phase into a monoclinic phase in the zirconium oxide surface. Prepared samples were analyzed by means of X-ray diffraction (XRD), which confirmed the phenomenon of transition. Measurements of the wetting angle and the calculated values of the surface free energy (SFE) showed no significant differences between the samples subjected to each treatment
Endogenous Enzymatic Activity of Primary and Permanent Dentine
Authors: Maravic Tatjana , Breschi Lorenzo , Paganelli Federica , Bonetti Giulio , Martina Stefano , Di Giorgio Gianni , Bossù Maurizio , Polimeni Antonella , Checchi Vittorio , Generali Luigi , Tay Franklin , Cadenaro Milena , Mazzoni Annalisa
Published: July 2021

Matrix metalloproteinases (MMPs) play an important role in tooth development and influence caries development and hybrid layer degradation. Literature is scant on the differences in the activity of MMPs between primary and permanent dentine. Accordingly, the aim of the present study was to investigate endogenous gelatinolytic activity in primary and permanent dentine. Separate batches of dentine powder were obtained from intact human primary and permanent molars (n = 6). Each batch was divided in two subgroups: (1) mineralised; and (2) demineralised with 10% H3PO4. After protein extraction, gelatine zymography was performed. Furthermore, in situ zymography was performed on dentine sections of the same groups (n = 3). The slices were polished, covered with fluorescein-conjugated gelatine and evaluated using a confocal microscope. In situ zymography data were analysed using two-way analysis of variance and post hoc Holm–Šidák statistics (α = 0.05). Primary dentine showed poorly defined bands in the zymograms that vaguely corresponded to the pro-form and active form of MMP-2 and the pro-form of MMP-9. In permanent dentine, demineralised powder demonstrated stronger gelatinolytic activity than mineralised powder. In situ zymography identified stronger enzymatic activity in primary etched dentine (p < 0.05). Stronger enzymatic activity recorded in primary dentine may be related to the differences in morphology and composition between primary and permanent dentine.
Influence of the Nb and V Addition on the Microstructure and Corrosion Resistance of the Fe-B-Co-Si Alloy
Authors: Mech Rafał , Gąsiorek Jolanta , Łaszcz Amadeusz , Babiarczuk Bartosz
Published: July 2021

The paper presents a comparison of the results of the corrosion resistance for three Fe-B-Co-Si-based newly developed alloys with the addition of Nb and V. The corrosion performance differences and microstructure variations were systematically studied using scanning electron microscope, electric corrosion equipment, X-ray diffractometer, and differential calorimeter. It has been shown that each alloying addition increased the corrosion resistance. The highest corrosion resistance obtained by potentiodynamic polarization was found for the alloy with both Nb and V addons (Fe57Co10B20Si5Nb4V4) and lowest in the case of the basic four-element Fe62Co15B14Si9 material. This shows that the proper choice of additions is of significant influence on the final performance of the alloy and allows tailoring of the material for specific applications.
Analyzing the Effects of the Kinematic System on the Quality of Holes Drilled in 42CrMo4 + QT Steel
Authors: Bronis Mateusz , Miko Edward , Nowakowski Lukasz
Published: July 2021

This article discusses the relationship between the kinematic system used in drilling and the quality of through-holes. The drilling was done on a CTX Alpha 500 universal turning center using a TiAlN-coated 6.0 mm drill bit with internal cooling, mounted in a driven tool holder. The holes were cut in cylindrical 42CrMo4 + QT steel samples measuring 30 mm in diameter and 30 mm in length. Three types of hole-drilling kinematic systems were considered. The first consisted of a fixed workpiece and a tool performing rotary (primary) and linear motions. In the second system, the workpiece rotated (primary motion) while the tool moved linearly. In the third system, the workpiece and the tool rotated in opposite directions; the tool also moved linearly. The analysis was carried out for four output parameters characterizing the hole quality (i.e., cylindricity, straightness, roundness, and diameter errors). The experiment was designed using the Taguchi approach (orthogonal array). ANOVA multi-factor statistical analysis was used to determine the influence of the input parameters (cutting speed, feed per revolution and type of kinematic system) on the geometrical and dimensional errors of the hole. From the analysis, it is evident that the kinematic system had a significant effect on the hole roundness error.
Investigation into the Effect of Spinel Pigments on the Photostability and Combustion Properties of Ethylene-Norbornene Copolymer
Authors: Kuśmierek Małgorzata , Szadkowski Bolesław , Rybiński Przemysław , Śliwka-Kaszyńska Magdalena , Prochoń Mirosława , Syrek Bartłomiej , Marzec Anna
Published: July 2021

Multicolor ethylene-norbornene (EN) composites filled with three different spinel pigments (Cobalt Green-PG50, Zinc Iron Yellow-PY 119, Praseodym Yellow-PY159) were prepared by melt mixing and characterized in terms of their stability under destructive environmental conditions. The EN films were subjected to accelerated aging by ultraviolet (UV) photooxidation for 300 h, 600 h, or 900 h. The mechanical performance of the EN composites was investigated in static and dynamic mechanical tests. The morphologies of the EN samples and their color changes during the aging process were evaluated by scanning electron microscopy (SEM) and spectrophotometric measurements. Fourier transform infrared (FTIR) spectroscopy was applied to determine the amount of carbonyl groups resulting from surface oxidation at different aging times. The effects of the spinel pigments on the thermal stability and combustion properties of the multicolor polymer composites were also assessed, and compared with a sample containing the organic Pigment Yellow 139 (PY139). The results show that the color changes (ΔE) in the spinel pigments were minor in comparison to those in the organic pigment (PY139) and the reference film. The Zinc Yellow (PY119) pigment was the most effective stabilizer of EN copolymer. Moreover, the spinel pigments had a positive effect on the flame retardancy of the EN composites. Microcombustion tests (MCC) showed that the incorporation of both the spinels and the organic pigment PY139 into the EN matrix reduced the heat release rate (HRR) and total heat release (THR) parameters.