bluuebunny/crossref_metadata_2025 huggingface dataset:
Acute kidney injury, renal impairment and renal failure associated with sodium glucose co‐transporter‐2 inhibitors in at‐risk groups: A systematic review
Authors: Ziser Kate E. D. , Livori Adam C. , Morton Jedidiah I. , Prosser Adaire , Ilomäki Jenni , Wood Stephen J.
Published: May 2024

Randomized controlled trials (RCTs) show a reduction in acute kidney injury, renal impairment and acute renal failure after initiation of a sodium glucose cotransporter‐2 inhibitor. Observational literature on the association is conflicting, but important to understand for populations with a higher risk of medication‐related adverse renal events. We aimed to systematically review the literature to summarize the association between sodium glucose cotransporter‐2 inhibitor use and acute kidney injury, renal impairment and acute renal failure in three at‐risk groups: older people aged >65 years, people with heart failure and people with reduced renal function. A systematic search of Embase (1974 until 23 February 2024) and PubMed (1946 until 23 February 2024) was performed. RCTs were included if they reported numbers of acute kidney injury or acute renal failure in people using sodium glucose cotransporter‐2 inhibitors compared to other diabetic therapies. Studies needed to report results by level of renal function, heart failure status or age. Of 922 results, eight studies were included. The absolute risk of acute kidney injury or acute renal failure was higher in people >65 years compared to those <65 years, higher in people with heart failure (vs without) and higher in people with reduced kidney function (vs preserved kidney function), but insufficient evidence to determine if the relative effect of sodium glucose cotransporter‐2 inhibitors on this risk was similar for each group. At‐risk cohorts are associated with a higher incidence of acute kidney problems in users of sodium glucose cotransporter‐2 inhibitors.
Safety evaluation of adenosine, lidocaine and magnesium (ALM) intranasal therapy toward human nasal epithelial cells in vitro
Authors: Morris Jodie L. , Letson Hayley L. , Dobson Geoffrey P.
Published: May 2024

Adenosine, lidocaine and Mg2+ (ALM) solution is an emerging therapy that reduces secondary injury after intravenous administration in experimental models of traumatic brain injury (TBI). Intranasal delivery of ALM may offer an alternative route for rapid, point‐of‐care management of TBI. As a preliminary safety screen, we evaluated whether ALM exerts cytotoxic or inflammatory effects on primary human nasal epithelial cells (pHNEC) in vitro. Submerged monolayers and air–liquid interface cultures of pHNEC were exposed to media only, normal saline only, therapeutic ALM or supratherapeutic ALM for 15 or 60 min. Safety was measured through viability, cytotoxicity, apoptosis, cellular and mitochondrial stress, and inflammatory mediator secretion assays. No differences were found in viability or cytotoxicity in cultures exposed to saline or ALM for up to 60 min, with no evidence of apoptosis after exposure to supratherapeutic ALM concentrations. Despite comparable inflammatory cytokine secretion profiles and mitochondrial activity, cellular stress responses were significantly lower in cultures exposed to ALM than saline. In summary, data show ALM therapy has neither adverse toxic nor inflammatory effects on human nasal epithelial cells, setting the stage for in vivo toxicity studies and possible clinical translation of intranasal ALM therapy for TBI treatment.
Riverscape features determining the genetic structure of the Yellow‐Spotted Amazon River Turtle (Podocnemis unifilis) in Brazilian Amazonia
Authors: Agostini Maria Augusta Paes , Machado Arielli Fabrício , Ritter Camila Duarte , Félix‐Silva Daniely , Pezzuti Juarez C. B. , Andrade Paulo César Machado , Pantoja‐Lima Jackson , da Silva Viana Maria das Neves , dos Santos Monjeló Luiz Alberto , Vogt Richard C. , de Oliveira Junior Waldesse Piragé , Farias Izeni Pires
Published: May 2024

Landscape characteristics can influence gene flow depending on the species ability to disperse. This imposes different levels of resistance to movement, determining the genetic structure and diversity of populations. Waterfalls and rapids in the Amazon basin have been suggested as contributing factors to the diversification and genetic structure of freshwater species, including turtles. Thus, along some main rivers of the Brazilian Amazon: Juruá, Madeira, Amazonas, Trombetas, Tapajós, Xingu, Tocantins, and Araguaia, we evaluated the spatial distribution of genetic diversity and characterized the population structure of Podocnemis unifilis, a widely distributed and endangered Amazonian turtle, using the mitochondrial DNA control region. We tested for isolation by geographic distance and by resistance models through an integrative approach using genetic, geographic, and ecological data, comparing these matrices with genetic distance. We found heterogeneous levels of genetic diversity and no spatial patterns. Results supported the isolation by resistance hypothesis, where the movement of individuals was influenced by freshwater, vegetational, climatological, and geomorphological variables, explaining the genetic distance of P. unifilis. Rapids and waterfalls impose greater resistance although they are barriers with different levels of permeability to the P. unifilis gene flow. Thus, the idiosyncrasies of each sub‐basin must be considered in the interpretation of the patterns found in assessing phylogeographic history.

Abstract in Portuguese is available with online material.
Mammal diversity responses to anthropic, environmental, and seasonal changes within Caatinga seasonal dry forest landscapes
Authors: Polli Daiana Jeronimo , dos Santos Juliana Silveira , Palmeira Francesca Belem Lopes , Conciani Dhemerson E. , de Campos Cláudia Bueno , Ribeiro Milton Cezar
Published: May 2024

Caatinga's conservation and biodiversity are threatened due to the intensification of anthropic activities and climate change. The mammals have different responses to seasonal and anthropic changes, however particularly in Caatinga, these effects are still poorly understood. We assessed the influence of anthropic (distance from urban areas and wind farms), environmental (distance from water), and seasonal (Normalized Difference Vegetation Index (NDVI) and land surface temperature (LST)) variables on the number of records and richness of medium and large‐sized mammals in Brazilian Caatinga. We used camera traps in 2016–2017 and 2018–2019, estimated the variation (cv) of NDVI and LST, and generated Euclidean distance maps to anthropic and environmental variables at 250, 500, and 1000 m spatial scales. We performed Generalized Linear Models, used the Akaike information criterion, and calculated model averaging to assess the strength and direction of effect and the uncertainties of the winner models, respectively. The distance from wind farms and maximum LST had a noticeable effect on the number of records and total richness. The distance from wind farms had a negative effect on the records of Dicotyles tajacu and a positive effect on the records of Leopardus pardalis and richness. The maximum LST had a negative effect on the records of L. pardalis and a positive effect on the records of Puma concolor and Cerdocyon thous. Our results emphasize that an unsustainable expansion of wind farms is likely to compromise mammal diversity. We found an opposite pattern for some species regarding LST. However, it is important to highlight that the conservation of vegetation areas on the top of mountains and springs, and the installation of artificial water sources are important strategies to mitigate the impacts of high temperatures on mammals' biodiversity in Caatinga.

Abstract in Portuguese is available with online material.
My cave, my rules: Specialization, modularity, and topology of bat‐fly interactions in hot and cold caves from eastern South America
Authors: Urbieta Gustavo Lima , da Cunha Tavares Valéria
Published: May 2024

Caves provide relatively stable and advantageous roosting sites for bats compared to more open roosts, like tree foliage. This environment may have the drawback of facilitating interactions with their ectoparasites due to the confined spaces. Understanding the structure of interactions between bats, acting as hosts, and bat flies, serving as parasites in cave ecosystems, is a crucial first step in deciphering the roles of each species (pullers and pushers) within the networks that form in subterranean ecosystems. Here, we describe and evaluate the network structures of bat‐fly interactions in two distinct cave systems: cold caves (n = 10), also known as bat caves, and hot caves (n = 6). Based on the records of 700 bats from 16 species and 1.412 bat flies from 30 species we uncovered highly distinct topologies comparing hot and cold bat caves that differed also in terms of interactions, specializations, and modularity. We found relatively lower specialization and modularity in hot caves compared to the cold caves, which may be associated to the bat composition and the cave microclimate. Bat flies were highly species‐specific in relation to their bat hosts and dependent on the bats in both hot and cold caves systems. The differences in network structure and at the species level between the bat (cold) and hot caves systems suggest that bat‐fly interactions are shaped by the host species' composition and by the cave system type. Those differences stem from each bat species' adaptation to extreme cave microclimates and their species‐specific roosting behaviors.

Abstract in Portuguese is available with online material.
Social Network Enhancement: Bridging and Bonding the Social Networks of Child Welfare Involved Youth
Authors: Okland Idun , Oterholm Inger , Brady Eavan
Published: May 2024

A supportive social network is crucial for the well‐being of child welfare involved youth; however, their social networks are often small and/or unable to provide necessary support. Child welfare workers can play an important role in strengthening and expanding the social networks of youth. In this Norwegian study, 15 child welfare workers' experiences with social network enhancement were examined using semi‐structured interviews. Data analysis applied a social capital lens to identify whether and how this framework could enhance our understanding of this issue. Study findings highlight three main objectives, implemented to (a) strengthening the youth's existing network, (b) supporting the youth to take part in (new) social arenas and (c) cultivating the youth's social skills and relationship building. Findings suggest that this approach can contribute to the development of bonding and bridging social capital. Within all objectives and associated strategies, trusting relationships between youth and child welfare workers were considered a prerequisite for success. Several barriers and impeding factors were also identified, such as the limited size of the social networks or lack of social skills. These barriers show how social network enhancement can be complicated, in actual practice.
Patterns of antibiotic prescription in implant dentistry and antibiotic resistance awareness among European dentists: A questionnaire‐based study
Authors: Becker Kathrin , Gurzawska‐Comis Katarzyna , Klinge Björn , Lund Bodil , Brunello Giulia
Published: May 2024

Objectives

Antimicrobial resistance is an alarming global public health concern, threatening the effective treatment of common infections. This phenomenon is driven by the improper prescription of antibiotics. This study aimed to elucidate the patterns of antibiotic prescription in implant dentistry among European dentists and their awareness of antibiotic resistance.

Materials and Methods

An anonymous online validated questionnaire was distributed via e‐mail to 6431 recipients through the European Association for Osseointegration. It comprised of 17 structured questions investigating demographic variables, working environment, clinical experience, attitude towards antibiotic prescription in particular in relation to implant dentistry and COVID‐19 pandemic, and awareness of antibiotic resistance. Data were collected from April to May 2023.

Results

281 dentists from 33 European countries completed the survey. Almost 80% affirmed to routinely prescribe antibiotics as prophylaxis as well as after dental implant placement, especially in medically compromised patients or in cases of bone grafting. Amoxicillin, alone (61%) or in combination with clavulanic acid (56%), was the most common antibiotic of choice. Awareness of penicillin resistance among respondents was high. For peri‐implantitis treatment, more than half reported the use of systemic antibiotics. The large majority (95%) did not prescribe more antibiotics since the beginning of COVID‐19 pandemic. Less than 40% declared to follow national guidelines for antibiotic prescription.

Conclusion

This survey revealed a high prescription rate of antibiotics in implant dentistry, despite the awareness about antibiotic resistance among the respondents. The development and adherence to European guidelines has been identified as a potential strategy for improving antimicrobial stewardship.
Transthoracic echocardiographic Doppler metrics in evaluating bioprosthetic tricuspid valve dysfunction
Authors: Zhang Xin , Peng Lingli , Fang Lingyun , Xu Jia , Wang Jing , Sun Wei , Gao Tang , Li Yuman , Zhang Li , Lv Qing , Xie Mingxing , Wu Wenqian
Published: May 2024

Purpose

There is currently limited information on the utility of transthoracic echocardiography (TTE)‐derived Doppler parameters for assessing bioprosthetic tricuspid valve (BTV) dysfunction. Our study aimed to establish the precision and appropriate reference ranges for routinely collected transthoracic Doppler parameters in the assessment of BTV dysfunction.

Methods

We retrospectively evaluated 100 BTV patients who underwent TTE. Based on redo surgical confirmation or more than 2 repeat TTE or transesophageal echocardiography (TEE) examinations, patients were allocated to normal (n = 61), regurgitant (n = 24), or stenotic (n = 15) BTV group. Univariate and multivariate binary logistic regression were performed to identify TTE Doppler parameters that detected BTV dysfunction.

Results

The VTI ratio (VTITV/VTILVOT) was the most accurate Doppler parameter for detecting BTV dysfunction, with a ratio of >2.8 showing 84.6% sensitivity and 90.2% specificity. VTI ratio > 3.2, mean gradient (MGTV) > 6.2 mmHg and pressure half‐time > 218 ms detected significant BTV stenosis, with sensitivities of 100%, 93.3% and 93.3% and specificities of 82.4%, 75.3% and 87.1%, respectively. After multivariate analysis, the VTI ratio > 2.8 (OR = 9.00, 95% CI = 2.13–41.61, p = .003) and MGTV > 5.1 mmHg (OR = 6.50, 95% CI = 1.69–27.78, p = .008) were the independent associations of BTV dysfunction. With these cutoff values, 75.0%–92.2% of normal and 62.5%–96.0% of dysfunctional BTV were identified.

Conclusions

Doppler parameters from TTE can accurately identify BTV dysfunction, particularly with VTI ratio > 2.8 and MGTV > 5.1 mmHg, to assess the need for additional testing with TEE.
Identifying and managing treatment resistance early with the integration of a clozapine clinic within an early intervention for psychosis service
Authors: O'Donoghue Brian , Mora Linda , Bismark Marie , Thompson Andrew , McGorry Patrick
Published: May 2024

Background

Despite being the most effective antipsychotic medication for treatment‐resistant psychosis, clozapine is often under‐utilized with long delays to initiation.

Aims

This study aimed to determine whether the integration of a clozapine clinic within an early intervention for psychosis service resulted in a change in the rate and time to initiation of clozapine, the number of trials of different antipsychotic medications prior to clozapine, community initiation and discontinuation rates.

Methods

A clozapine clinic was established in the Early Psychosis Prevention and Intervention Centre in Melbourne. This was a pre‐ and post‐evaluation study design. The ‘clozapine clinic’ cohort included those who commenced on clozapine from 01 January 2016 to 30 June 2018.

Results

Prior to the clozapine clinic, 24 young people commenced clozapine over the 30‐month period compared to 36 in the clozapine clinic cohort (RR = 1.30, 95% CI: 0.75–2.28, p = .32). In the pre‐clozapine clinic cohort, 4.6% of all those with a first episode of psychosis were commenced on clozapine compared to 6% in the clozapine clinic cohort. Prior to the clozapine clinic, the median time to the commencement of clozapine was 72 weeks (IQR: 38, 87), compared to 53.5 weeks (IQR: 38, 81.5) in the clozapine clinic (Z = −0.86, p = .393). The mean number of different antipsychotic medications prior to commencing clozapine remained stable at 3.2 (SD ± 1.1) in both cohorts (t = −0.20, p = .841). There was a lower rate of discontinuation in the clozapine clinic (43.5% vs. 14.7%, HR = 0.30, 95% CI: 0.09–0.98, p = .046).

Conclusions

While this study was underpowered and there are limitations to the naturalistic study design, the findings lend support to the integration of a clozapine clinic within early intervention for psychosis services.
Molecular cytogenetic screening of sex chromosome abnormalities in young horse populations
Authors: Bugno‐Poniewierska Monika , Jankowska Magdalena , Raudsepp Terje , Kowalska Katarzyna , Pawlina‐Tyszko Klaudia , Szmatola Tomasz
Published: May 2024

Background

Chromosomal abnormalities occur in the equine population at a rate of approximately 2%. The use of molecular cytogenetic techniques allows a more accurate identification of chromosomal abnormalities, especially those with a low rate of abnormal metaphases, demonstrating that the actual incidence in equine populations is higher.

Objectives

Estimation of the number of carriers of karyotypic abnormalities in a sample from a population of young horses of various breeds, using molecular cytogenetic techniques.

Study design

Cross‐sectional.

Methods

Venous blood samples were collected from 500 young horses representing 5 breeds (Purebred Arabian, Hucul, Polish primitive horse [Konik], Małopolska, Coldblood, Silesian). Chromosomes and DNA were obtained from blood lymphocytes and evaluated by fluorescence in situ hybridisation (FISH) and PCR, using probes and markers for the sex chromosomes and select autosomes.

Results

Nineteen horses, 18 mares and 1 stallion, were diagnosed with different chromosomal abnormalities: 17 cases of mosaic forms of sex chromosome aneuploidies with a very low incidence (0.6%–4.7%), one case of a SRY‐negative 64,XY sex reversal mare, and one mare with X‐autosome translocation. The percentage of sex chromosomal aberrations was established as 3.8% in the whole population, 6.08% in females and 0.49% in males.

Main limitations

Limited sample size, confined to horses from Poland.

Conclusions

The rate of sex chromosomal abnormalities we identified was almost double that reported in previous population studies that used classical chromosome staining techniques. FISH allowed the detection of aneuploid cell lines which had a very low incidence. The FISH technique is a faster and more precise method for karyotype examination; however, it is usually focused on only one or two chromosomes while banding karyotyping includes the entire chromosome set.
Catch uncertainty and recreational fishing attraction: Propositions and future research directions
Authors: Arlinghaus Robert
Published: May 2024

Why do people fish for recreation? Social science literature suggests that both catch (e.g., number or sizes of fish) and non‐catch dimensions (e.g., nature experience, temporary escape) play a role. After reviewing the literature from environmental psychology, neuroscience, anthropology, recreational fisher motivation research as well as popular fishing books, I find that the opposite of catching fish, more specifically the uncertainty of the catch, maybe another, perhaps fundamental force that explains the attraction of the activity to millions of people. There appears to be strong utility in the gaming nature of the activity. This quality may contribute to explain various patterns that are well known, e.g., the overinvestment of time and money by recreational fishers that drastically exceed the market value of fish, the lack of self‐regulation of a local recreational fishery in terms of effort being spent also on low stock sizes, the disutility associated with providing certain catch probability information, diminishing marginal utility return for increasing catch rates, management regulations that make fishing harder than necessary, suboptimal satisfaction despite rising catch rates, and finally the dominance of men among populations of recreational fishers. I present a serious of testable propositions and call for a novel research focus that seeks to better understand what makes catch ambiguity attractive psychologically and emotionally.
The educational resources and needs of mothers of preschool children from two socioeconomic levels
Authors: Han‐Uysal Senem , Kızıltepe Zeynep , Akmehmet‐Şekerler Sibel , Buran Aylin
Published: May 2024

Objective

This study aimed to investigate the educational resources and needs of mothers of children aged 60 to 72 months and the differences between those of high and low socioeconomic status (SES).

Background

Parents have a crucial impact on their children's development in the preschool years. Mothers are especially challenged by the variety of information sources available and struggle with parenting decisions.

Method

Thirty‐three structured interviews with mothers were carried out in one private and one public school, representing high and low SES, respectively.

Findings

Between the two SES groups, both similarities and differences were observed in terms of their educational resources and needs. The following themes emerged in the study: general sources of parenting information, challenges, the first source to consult when problems occur, topics preferred in a parent education program, and the phase of maternity in which they preferred to receive training. The two SES groups were similar in terms of their main challenges, the topics preferred in a parent education program, and the phase of maternity in which they preferred to receive education. Differences were observed in terms of the sources of parenting information, the first source to consult when problems occur, and some of the preferred topics of a parent education program.

Implications

These findings may be useful in revisiting existing parent education programs, or in creating new ones, for the caregivers of preschool children.
Cannibalism facilitates and sustains the invasion of top‐predator fish (Cichlidae: Cichla) in human‐modified ecosystems
Authors: Guedes Gustavo Henrique Soares , Franco Ana Clara Sampaio , dos Santos Luciano Neves , dos Santos Alejandra Filippo Gonzalez Neves , Araújo Francisco Gerson
Published: May 2024

The strategies that allow invasive alien species to perpetuate themselves in the long term are still poorly understood in invasion science. Considering top predators, a key issue is how they overcome the prey decline which they have caused during the invasion process. We investigate factors related to the persistence, impacts and long‐term success of the emerging peacock bass (Cichlidae: Cichla) invasion, a fish native to the Amazon biome that has been introduced globally.

We analysed a robust temporal database that includes comparisons (1994–2002 vs. 2015–2017) on the diet of Cichla ocellaris, the abundance of this invader, its prey and competitors in a human‐modified ecosystem. In addition, we compare the frequency of cannibalism (presence or absence) between native versus non‐native populations, and between natural versus human‐modified ecosystems in the American continent. Four hypotheses were tested: (i) prey and competitor populations decrease through time as a result of predation impacts; (ii) cannibalism is size‐ and density‐dependent, and increases after native prey decline; (iii) cannibalism is more frequent in non‐native populations; and (iv) the abundance of invader decreases over time because of reduced resources and increased cannibalism.

Our results revealed that the impacts of introducing this predator into communities are temporally cumulative and continue to evolve even after 70 years since its introduction. These impacts have led to dramatic reductions in prey availability, resulting in widespread cannibalism. Adult individuals preying on juveniles in areas with higher abundances of peacock bass indicated that cannibalism is size‐ and density‐dependent. Cannibalism was more frequent in non‐native populations and in human‐modified ecosystems. A complex feedback process (reproduce and cannibalise frequently) may be responsible for overcoming long‐term resource scarcity, and the cannibalism may not be a regulatory force strong enough to surpass the reproductive success of the population.

Our findings at the local and continental scale converge to confirm the cannibalism as an adaptive invasiveness strategy that facilitates the invasion process and makes populations self‐sustainable in the long term, which may jeopardise the efficacy of management actions for these top predators introduced globally.
Density‐dependent trophic consequences of invasive mrigal carp on native mud carp in Chinese fresh waters
Authors: Zhang Yingqiu , Li Jie , Chen Fangcan , Li Yuefei , Dai Shouhui , Britton J. Robert
Published: May 2024

Understanding the strength, direction and consequences of the trophic impacts of invasive fish on functionally analogous native fishes is important in invasion impact assessment and for informing management programmes. As functionally analogous native and invasive fishes can share prey resources, there is high potential for competitive interactions, which could affect trophic niche sizes and positions.

The highly invasive mrigal carp Cirrhinus mrigala and native mud carp Cirrhinus molitorella now co‐exist in many freshwaters in southern China, raising concern on the consequences for mud carp. The trophic interactions of the two species and their consequences (for growth rates and trophic niche sizes as the isotopic niche, calculated using δ13C and δ15N) were tested in a pond enclosure experiment based on additive and substitutive treatments using fish in allopatric and sympatric contexts, with results compared with invaded wild communities.

In the experiment, specific growth rates decreased significantly as fish abundance in treatments increased. These density‐dependent effects were observed in both species and were independent of whether the treatment was allopatric or sympatric. In allopatric treatments, isotopic niches of mud carp increased in size with fish number. Conversely, in sympatric treatments, mud carp isotopic niche size decreased with increased mrigal carp number. For mrigal carp, isotopic niche sizes decreased as fish number increased in both allopatric and sympatric treatments.

In sympatric experiment treatments, the isotopic niches of mud carp and mrigal carp were always divergent. This was, however, in contrast to four invaded wild communities, where the isotopic niches of the two fishes always had some overlap.

Experimental and field data suggest that although native mud carp could be impacted by mrigal carp, these impacts might only manifest when the species are at very high abundances and inter‐specific competitive interactions could be intense. These results also suggest the two species have mechanisms that reduce their inter‐specific interactions to minimise their competitive interactions, thus any ecological impacts from mrigal carp might manifest more from indirect than direct processes.
Study of New CGSP‐P Series Phosphate Matrix Reference Materials for LA‐ICP‐MS
Authors: Sun Dongyang , Fan Chenzi , Guo Wei , Zhao Linghao , Zhan Xiuchun , Hu Mingyue
Published: May 2024

A series of three CGSP‐P phosphate matrix reference materials, with variable element mass fractions, were synthesised by co‐precipitation with CaCl2 and (NH4)3PO4 to form a hydroxylapatite matrix. Each powder sample was packaged into multiple bottles and pressed into tablets as replicates. The within‐bottle homogeneity was evaluated by the repeatability of element mass fractions of six spots in per tablet of CGSP‐Ps by LA‐ICP‐MS. For the between‐bottle homogeneity evaluation, one approach adopted the % RSD of element mass fractions in twelve different tablets from twelve bottles in the same reference material in comparison with the repeatability field of LA‐ICP‐MS analyses obtained from homogeneous glasses, and the other used a one‐way analysis of variance (ANOVA) approach. Most of the elements (e.g., Mg, Ca, P, Mn and REE) were homogenous within 10% RSD, and other elements (e.g., Si, Al, K, Rb, Cs and Ni) were considered heterogeneous (with > 20% RSD). All of the elements passed the t‐test except Ni in CGSP‐P3, and V, Cr, Ni, Zr, Gd, Dy and Th in CGSP‐P4 after a 29‐month period stability examination by LA‐ICP‐MS. The preliminary reference values and standard uncertainties for CGSP‐Ps are given with a network of methods and eight laboratories by bulk analysis according to ISO Guide 35:2006 and JJF 1343‐2012.
Revisiting the gene mutations and protein profile of WT 9‐12: An autosomal dominant polycystic kidney disease cell line
Authors: Chai Hwa Chia , Mahendran Rhubaniya , Ong Kien Chai , Chua Kek Heng
Published: May 2024

WT 9‐12 is one of the cell lines commonly used for autosomal dominant polycystic kidney disease (ADPKD) studies. Previous studies had described the PKD gene mutations and polycystin expression in WT 9‐12. Nonetheless, the mutations occurring in other ADPKD‐associated genes have not been investigated. This study aims to revisit these mutations and protein profile of WT 9‐12. Whole genome sequencing verified the presence of truncation mutation at amino acid 2556 (Q2556X) in PKD1 gene of WT 9‐12. Besides, those variations with high impacts included single nucleotide polymorphisms (rs8054182, rs117006360, and rs12925771) and insertions and deletions (InDels) (rs145602984 and rs55980345) in PKD1L2; InDel (rs1296698195) in PKD1L3; and copy number variations in GANAB. Protein profiles generated from the total proteins of WT 9‐12 and HK‐2 cells were compared using isobaric tags for relative and absolute quantitation (iTRAQ) analysis. Polycystin‐1 was absent in WT 9‐12. The gene ontology enrichment and reactome pathway analyses revealed that the upregulated and downregulated proteins of WT 9‐12 relative to HK‐2 cell line leaded to signaling pathways related to immune response and amino acid metabolism, respectively. The ADPKD‐related mutations and signaling pathways associated with differentially expressed proteins in WT 9‐12 may help researchers in cell line selection for their studies.
Treatment of thrombotic cardiovascular diseases in people with haemophilia: A Japanese consensus study
Authors: Nagao Azusa , Chikasawa Yushi , Hiroi Yukio , Ieko Masahiro
Published: May 2024

Introduction

Cardiovascular diseases (CVD) that require long‐term anticoagulant and antiplatelet therapy presents a problem in people with haemophilia (PWH) who receive factor replacement therapy to reduce bleeding risk. Currently, there are no Japanese guidelines for the management of PWH with CVD.

Aim

To develop expert guidance on managing CVD in PWH in Japan.

Methods

A steering committee of four experts (two haemophilia specialists, one thrombosis specialist, one cardiologist) identified 44 statements related to five key themes. An online questionnaire was produced comprising a mix of 4‐point Likert scale and multiple‐choice questions that was sent to specialists in the management of PWH with CVD in Japan. Consensus was defined as high or very high if a respective ≥75% or ≥90% of respondents agreed with a statement.

Results

Of 16 potential respondents, responses were received from 15 specialists. Of the Likert scale questions, 71% (29/41) achieved ≥90% agreement (very strong agreement), 17% (7/41) achieved 75%–89% agreement (strong agreement) and 15% (6/41) did not achieve consensus agreement. The three multiple‐choice questions failed to identify a strong preference. Agreement on specific target trough clotting factor levels for managing certain clinical situations, such as when in the presence of non‐valvular atrial fibrillation or myocardial infarction, was also achieved.

Conclusion

The results of this consensus study provide a framework for cardiologists and haematologists to manage PWH who are at risk of, or who have, CVD. Implementation of the recommendations provided herein may improve outcomes for PWH with CVD.
Machine learning identifies factors most associated with seeking medical care for migraine: Results of the OVERCOME (US) study
Authors: Ashina Sait , Muenzel E. Jolanda , Nicholson Robert A. , Zagar Anthony J. , Buse Dawn C. , Reed Michael L. , Shapiro Robert E. , Hutchinson Susan , Pearlman Eric M. , Lipton Richard B.
Published: May 2024

Objective

Utilize machine learning models to identify factors associated with seeking medical care for migraine.

Background

Migraine is a leading cause of disability worldwide, yet many people with migraine do not seek medical care.

Methods

The web‐based survey, ObserVational survey of the Epidemiology, tReatment and Care Of MigrainE (US), annually recruited demographically representative samples of the US adult population (2018–2020). Respondents with active migraine were identified via a validated diagnostic questionnaire and/or a self‐reported medical diagnosis of migraine, and were then asked if they had consulted a healthcare professional for their headaches in the previous 12 months (i.e., “seeking care”). This included in‐person/telephone/or e‐visit at Primary Care, Specialty Care, or Emergency/Urgent Care locations. Supervised machine learning (Random Forest) and Least Absolute Shrinkage and Selection Operator (LASSO) algorithms identified 13/54 sociodemographic and clinical factors most associated with seeking medical care for migraine. Random Forest models complex relationships (including interactions) between predictor variables and a response. LASSO is also an efficient feature selection algorithm. Linear models were used to determine the multivariable association of those factors with seeking care.

Results

Among 61,826 persons with migraine, the mean age was 41.7 years (±14.8) and 31,529/61,826 (51.0%) sought medical care for migraine in the previous 12 months. Of those seeking care for migraine, 23,106/31,529 (73.3%) were female, 21,320/31,529 (67.6%) were White, and 28,030/31,529 (88.9%) had health insurance. Severe interictal burden (assessed via the Migraine Interictal Burden Scale‐4, MIBS‐4) occurred in 52.8% (16,657/31,529) of those seeking care and in 23.1% (6991/30,297) of those not seeking care; similar patterns were observed for severe migraine‐related disability (assessed via the Migraine Disability Assessment Scale, MIDAS) (36.7% [11,561/31,529] vs. 14.6% [4434/30,297]) and severe ictal cutaneous allodynia (assessed via the Allodynia Symptom Checklist, ASC‐12) (21.0% [6614/31,529] vs. 7.4% [2230/30,297]). Severe interictal burden (vs. none, OR 2.64, 95% CI [2.5, 2.8]); severe migraine‐related disability (vs. little/none, OR 2.2, 95% CI [2.0, 2.3]); and severe ictal allodynia (vs. none, OR 1.7, 95% CI [1.6, 1.8]) were strongly associated with seeking care for migraine.

Conclusions

Seeking medical care for migraine is associated with higher interictal burden, disability, and allodynia. These findings could support interventions to promote care‐seeking among people with migraine, encourage assessment of these factors during consultation, and prioritize these domains in selecting treatments and measuring their benefits.
German language adaptation of the Cogniphobia Scale for Headache Disorders (CS‐HD) and development of a new short form (CS‐HD‐6)
Authors: Klan Timo , Bräscher Anne‐Kathrin , Seng Elizabeth K. , Gaul Charly , Witthöft Michael
Published: May 2024

Objective

This study is part of the ODIN‐migraine (Optimization of Diagnostic Instruments in migraine) project. It is a secondary, a priori analysis of previously collected data, and aimed to assess the psychometric properties and factor structure of the Cogniphobia Scale for Headache Disorders (CS‐HD). We aimed to construct a German‐language version and a short version.

Background

Cogniphobia is the fear and avoidance of cognitive exertion, which the patient believes triggers or exacerbates headache. High cogniphobia may worsen the course of a headache disorder.

Methods

The 15‐item CS‐HD was translated into German and back translated in a masked form by a professional translator. Modifications were discussed and carried out in an expert panel. A cross‐sectional online survey including the CS‐HD and further self‐report questionnaires was conducted in a sample of N = 387 persons with migraine (364/387 [94.1%] female, M = 41.0 [SD = 13.0] years, migraine without aura: 152/387 [39.3%], migraine with aura: 85/387 [22.0%], and chronic migraine: 150/387 [38.8%]).

Results

Exploratory factor analysis resulted in two clearly interpretable factors (interictal and ictal cogniphobia). Confirmatory factor analysis yielded an acceptable to good model fit (χ2(89) = 117.87, p = 0.022, χ2/df = 1.32, RMSEA = 0.029, SRMR = 0.055, CFI = 0.996, TLI = 0.995). Item response theory‐based analysis resulted in the selection of six items for the short form (CS‐HD‐6). Reliability was acceptable to excellent (interictal cogniphobia subscale: ω = 0.92 [CS‐HD] or ω = 0.77 [CS‐HD‐6]; ictal cogniphobia subscale: ω = 0.77 [CS‐HD] or ω = 0.73 [CS‐HD‐6]). The pattern of correlations with established questionnaires confirmed convergent validity of both the CS‐HD and the CS‐HD‐6.

Conclusion

Both the CS‐HD and the CS‐HD‐6 have good psychometric properties and are suitable for the assessment of cogniphobia in migraine.
A real‐world prospective observational study of eptinezumab in Asian patients with migraine
Authors: Zhao Yi Jing , Ong Jonathan Jia Yuan , Sonu Sumit Kumar , Dang Jiaojiao , Ng Chai Ching , Herr Keira Joann , Bose Rohini , Jion Yasmin Idu
Published: May 2024

Objective

To evaluate the real‐world effectiveness of eptinezumab for migraine prevention in Asian patients.

Background

Eptinezumab is a monoclonal antibody that targets calcitonin gene‐related peptide (CGRP), a potent vasodilator with an important role in migraine pathophysiology. Although there is robust clinical evidence from pivotal Phase 3 placebo‐controlled trials of the efficacy of eptinezumab for migraine prevention, there are limited data on the real‐world effectiveness of eptinezumab in Asian patient cohorts.

Methods

This was a non‐interventional, prospective, multisite cohort study of adults with migraine (International Classification of Headache Disorders, 3rd edition criteria) in Singapore who were prescribed eptinezumab (100 mg at baseline and Month 3, administered intravenously) and were followed until Month 6. The primary endpoint was change from baseline in monthly migraine days (MMDs) at Month 3 and Month 6. Secondary endpoints were ≥30% and ≥50% responder rates, and change from baseline in the Headache Impact Test‐6 (HIT‐6), Migraine Disability Assessment (MIDAS), Migraine‐Specific Quality of Life (MSQ), patient‐identified most bothersome symptom (PI‐MBS), acute medication use at Month 3 and Month 6, and safety.

Results

Enrolled patients (completed = 29/30) had on average 3.4 (SD 2.9) previous preventive treatments; 29/30 of the patients had trialed at least one previous preventive treatment without benefit. Most had previously trialed oral preventives (87%, 26/30) and anti‐CGRP (70%, 21/30). Relative to baseline, mean MMDs decreased by 4.3 days (95% CI 2.1–6.4; p < 0.001) at Month 3 and 4.9 days (95% CI 2.1–7.7; p < 0.001) at Month 6. At Month 3 and Month 6, 14/30 (47%) and 15/29 (52%) of the patients were ≥30% responders, and 6/30 (20%) and 8/29 (28%) patients were ≥50% responders, respectively. The number of patients with severe life impairment based on the HIT‐6 score (total score 60–78) decreased from 24/30 (80%) at baseline to 19/30 (63%) at Month 3 and 19/29 (66%) at Month 6. The mean MIDAS score decreased by 24.6 points (95% CI 2.82–46.38; p = 0.028) at Month 6, and the mean MSQ score increased by 12.2 points (95% CI 5.18–19.20; p = 0.001) at Month 3 and 13.6 points (95% CI 4.58–22.66; p = 0.004) at Month 6. Most patients reported improvement in the PI‐MBS at Month 3 (73%, 22/30) and Month 6 (55%, 16/29). Acute medication use for headache relief decreased by 3.3 days/month (95% CI 1.0–5.6; p = 0.007) at Month 3 and 4.7 days/month (95% CI 1.7–7.7; p = 0.003) at Month 6. Treatment‐emergent adverse events (TEAEs) were reported in 16/30 (54%) patients, mostly mild/moderate in severity. No serious TEAEs led to treatment discontinuation.

Conclusion

Quarterly eptinezumab administration was effective and well‐tolerated in Asian patients with chronic migraine.
Effect of heating and germination on bioactive properties, phenolic compounds, fatty acid and mineral contents of quinoa (Chenopodium quinoa Willd.) seeds
Authors: Younis Mahmoud , Ahmed Isam A. Mohamed , AlJuhaimi Fahad , Wang Dongying , Uslu Nurhan , Özcan Mehmet Musa
Published: May 2024

In this study, the effect of roasting, boiling and germination processes on bioactive compounds, antioxidant activity, fatty acid and phenolic profiles and biogenic elements of quinoa seed and oils was assigned. The moisture amounts of boiled and germinated quinoa seeds were recorded to be significantly higher than the control. Also, the oil yield of quinoa seeds decreased compared with the control by roasting, boiling and germinating. The total phenolic and flavonoid quantities of quinoa seeds were assigned to be between 137.70 (germinated) and 315.00 (boiled) to 156.62 (boiled) and 402.38 mg/100 g (control), respectively. Antioxidant capacities of processed quinoa seeds were reported to be between 11.91 (germinated) and 14.43 mmol kg−1 (roasted). The highest total flavonoid and antioxidant capacities were recorded in the control group and roasted quinoa seeds. The highest decrease in total phenol amount of quinoa seeds was detected in germinated quinoa. Gallic acid and catechin values of quinoa seeds varied to be between 29.17 (boiled) and 38.00 mg/100 g (control) to 2.72 (germinated) and 137.26 mg/100 g (control), respectively. The rutin, ferulic acid and p‐coumaric acid contents of the processed (roasted, boiled and germinated) quinoa seeds gradually increased compared with the control. The highest palmitic, oleic, behenic and arachidonic acids were recorded in germinated quinoa oil, followed by the fatty acids of boiled, raw (control) and roasted quinoa seed oils in descending order. P and K amounts of quinoa were established to be between 2203.44 (roasted) and 3314.59 (control) to 4350.60 (boiled) and 6266.85 mg kg−1 (control), respectively.
A critical role for the nuclear protein Akirin in larval development in Henosepilachna vigintioctopunctata
Authors: Anjum Ahmad Ali , Lin Meng‐Jiao , Jin Lin , Li Guo‐Qing
Published: May 2024

Akirin is a nuclear protein that controls development in vertebrates and invertebrates. The function of Akirin has not been assessed in any Coleopteran insects. We found that high levels of akirin transcripts in Henosepilachna vigintioctopunctata, a serious Coleopteran potato defoliator (hereafter Hvakirin), were present at prepupal, pupal and adult stages, especially in larval foregut and fat body. RNA interference (RNAi) targeting Hvakirin impaired larval development. The Hvakirin RNAi larvae arrested development at the final larval instar stage. They remained as stunted larvae, gradually blackened and finally died. Moreover, the remodelling of gut and fat body was inhibited in the Hvakirin depleted larvae. Two layers of cuticles, old and newly formed, were noted in the dsegfp‐injected animals. In contrast, only a layer of cuticle was found in the dsakirin‐injected beetles, indicating the arrest of larval development. Furthermore, the expression of three transforming growth factor‐β cascade genes (Hvsmox, Hvmyo and Hvbabo), a 20‐hydroxyecdysone (20E) receptor gene (HvEcR) and six 20E response genes (HvHR3, HvHR4, HvE75, HvBrC, HvE93 and Hvftz‐f1) was significantly repressed, consistent with decreased 20E signalling. Conversely, the transcription of a juvenile hormone (JH) biosynthesis gene (Hvjhamt), a JH receptor gene (HvMet) and two JH response genes (HvKr‐h1 and HvHairy) was greatly enhanced. Our findings suggest a critical role of Akirin in larval development in H. vigintioctopunctata.
Systematic histology and evidence for the male reproductive activity of Amphibalanus amphitrite (Darwin, 1854)
Authors: Senarat Sinlapachai , Kaewnuan Romfa , Sudtongkong Chanyut , Boonyoung Piyakorn , Sukparangsi Woranop , Kongtueng Piyamat , Srivorakul Saralee , Wongkhamhaeng Koraon , Charoenphon Natthawut , lida Atsuo , Kenthao Anan
Published: May 2024

The striped acorn barnacle, Amphibalanus amphitrite, a taxon of Balanidae, is commonly found along Thailand's coastline. In this study, we used histological methods to describe the microscopic anatomy of A. amphitrite. All samples (n = 30) were obtained from the seagrass beds of Libong Island, Thailand, in the Andaman Sea. Histological observation of various longitudinal sections from specimens of A. amphitrite provides clear insights into the organization of organ systems in the species. The digestive tract was observed to be mainly divided into four subregions, including the cuticle‐lined mouth and esophagus, which is characterized by a high columnar epithelium, the stomach, and the intestine. The accessory organ, including the digestive gland, exhibited lobulated structures and was covered with low columnar epithelial cells on a thin layer of smooth muscle. Furthermore, we examined the reproductive system, including both the ovary and testes, as well as the process of gametogenesis. Testicular development was predominantly observed during active breeding seasons throughout the observation.
Spin coating high‐k multicomponent (Al,Ti,V,Zr,Hf)Ox films with sub‐nm EOT for MOS‐based electronic devices
Authors: Wang Yu‐Chen , Nguyen Van Dung , Chang Kao‐Shuo
Published: May 2024

The facile spin coating fabrication of high‐dielectric‐constant (high‐k) multicomponent (Al,Ti,V,Zr,Hf)Ox films with an equivalent oxide thickness of ≈0.95 nm and the investigation of their thermal stability and dielectric and electrical properties of the resulting advanced metal–oxide–semiconductor (MOS)‐based electronic devices are reported in this study. Various heating conditions, including drying following spin coating, annealing after drying, and forming gas annealing, were investigated to optimize the quality of the films and reduce film defects. The favorable film‐based MOS devices exhibited robust capacitance–voltage (C–V) and current–voltage (I–V) characteristics with a remarkable k value of approximately 62 at 1 kHz. The thermal stability of the films was affirmed through a rapid thermal annealing treatment at 900°C for 5 s and observation of nondegraded C–V and I–V curves. The electrical performance of the resulting MOS field‐effect transistors (MOSFETs), including a threshold voltage of 0.4 V, an on/off ratio of 106, a saturated mobility of 40.1 cm2 V−1 s−1, and a subthreshold swing of 66.7 mV dec−1, was obtained. Moreover, hole‐ and electron‐trapping measurements through negative and positive gate bias stress instabilities, respectively, indicate the reliable performance of our MOSFETs. Our results imply that solution‐based processes are promising for fabricating fundamental semiconductor devices.