bluuebunny/crossref_metadata_2025 huggingface dataset:
Lipid and Protein Oxidation of Brown Rice and Selenium-Rich Brown Rice during Storage
Authors: Zhang Minghui , Liu Kunlun
Published: December 2022

Selenium-rich rice has become one of the effective ways to increase people’s selenium intake. Selenium-containing proteins have higher antioxidant properties, which may lead to selenium-rich brown rice (Se-BR) having better storage stability than ordinary brown rice (BR). By measuring the peroxidation value, fatty acid value, carbonyl value and protein secondary structure, it was found that Se-BR had higher oxidation resistance stability than BR. The biological function of the differential proteins (DEPs) between ordinary brown rice stored for 0 days (BR-0) and 180 days (BR-6) as well as Se-rich brown rice stored for 0 days (Se-0) and 180 days (Se-6) was investigated by using iTRAQ. A total of 237, 235, 113 and 213 DEPs were identified from group A (BR-0/BR-6), group B (Se-0/Se-6), group C (BR-0/Se-0) and group D (BR-6/Se-6), respectively. Kyoto Encyclopedia of Genes and Genomes analysis showed that the DEPs were mainly enriched in glucose metabolism, tricarboxylic acid cycle, fatty acid biosynthesis and degradation, glutathione metabolism, sulfur metabolism, peroxisome and other metabolic pathways. This study provides theoretical support for the study of protein oxidation kinetics and storage quality control of brown rice during storage.
“I Just Want to Exist as Me”: Reflexivity and Our Duoethnographic Journey to Understanding the Self as Asian American and Asian Critical Scholars
Authors: Pedraza Chadrhyn Arevalo Agpalo , Park Chan Jeong
Published: December 2022

Critical research, such as that involving the deconstruction of monoracialism, aims to empower and elevate the voices of marginalized populations. When we engage in critical research, whether it be quantitative or qualitative, scholars must recognize how our own lived experiences might shape each stage of the research process. The purpose of this paper is twofold. First, we present scholars with a structured method using a conceptual mapping of social identities combined with written reflection and regularly scheduled debriefings to begin their own explorations of identity. Second, we present our experiences negotiating with monoracialism as we worked to understand our identities as Asian scholars. Through this process we discovered new perspectives on how we, along with our participants, have grappled with socially imposed notions of who we are as Asians.
Varying Doses of Rare-Earth-Metal-Based Neodymium Zirconate Zinc Sulfide Nanocomposite Disrupt Blood and Serum Parameters, as well as Markers of Oxidative Stress in the Selected Organs of Albino Mice
Authors: Khosa Tafheem , Ben Said Mourad , Rehman Zia , Ali Muhammad , Jamil Sania , Fatima Qandeel , Hussain Hafsa , Iqbal Rehana , Khan Adil , Iqbal Furhan
Published: December 2022

Despite extensive industrial use, the biocompatibility of nanocomposites has not been extensively explored. The present study was designed to report the effect of variable doses of a newly synthesized nanocomposite, Neodymium Zirconate Zinc Sulfide, on selective serum and complete blood count parameters and on the oxidative stress markers from the vital organs of albino mice. Albino mice (C57BL/6 strain, 5 weeks old) of both sexes were orally treated for 11 days, either with 10 mg (low dose) or 20 mg/mL saline/kg body weight (high dose) of Neodymium Zirconate Zinc Sulfide nanocomposite. A control group that was not treated with the nanocomposite but with saline solution was also maintained. Data analysis revealed that high-dose nanocomposite-treated male mice had significantly reduced hemoglobin concentration as compared to the control males. Female mice treated with both doses of nanocomposite had higher serum triglyceride levels than controls. High-dose-treated female mice had elevated serum cholesterol concentration compared to their saline-treated controls. Oxidative stress marker analysis from selected organs indicated that concentrations of malonaldehyde (MDA) in the kidney and liver, Superoxide dismutase (SOD) levels in the brain and catalase in the kidney of male mice treated with the nanocomposite were significantly higher than in the control group, whereas SOD in the heart, MDA in the heart and kidney and catalase levels in the kidney were significantly disrupted in female mice compared to their respective controls.
Utility of Cardiac Power Hemodynamic Measurements in the Evaluation and Risk Stratification of Cardiovascular Conditions
Authors: Farshadmand Jonathan , Lowy Zachary , Hai Ofek , Zeltser Roman , Makaryus Amgad
Published: December 2022

Despite numerous advancements in prevention, diagnosis and treatment, cardiovascular disease has remained the leading cause of mortality globally for the past 20 years. Part of the explanation for this trend is persistent difficulty in determining the severity of cardiac conditions in order to allow for the deployment of prompt therapies. This review seeks to determine the prognostic importance of cardiac power (CP) measurements, including cardiac power output (CPO) and cardiac power index (CPI), in various cardiac pathologies. CP was evaluated across respective disease-state categories which include cardiogenic shock (CS), septic shock, transcatheter aortic valve replacement (TAVR), heart failure (HF), post-myocardial infarction (MI), critical cardiac illness (CCI) and an “other” category. Literature review was undertaken of articles discussing CP in various conditions and this review found utility and prognostic significance in the evaluation of TAVR patients with a significant correlation between one-year mortality and CPI; in HF patients showing CPI and CPO as valuable tools to assess cardiac function in the acute setting; and, additionally, CPO was found to be an essential tool in patients with CCI, as the literature showed that CPO was statistically correlated with mortality. Cardiac power and the derived measures obtained from this relatively easily obtained variable can allow for essential estimations of prognostic outcomes in cardiac patients.
A Scoping Review on Long COVID-19: Physiological and Psychological Symptoms Post-Acute, Long-Post and Persistent Post COVID-19
Authors: Surapaneni Krishna Mohan , Singhal Manmohan , Saggu Sofia Rani , Bhatt Ashruti , Shunmathy Priya , Joshi Ashish
Published: December 2022

Background: The identification of persistent symptoms of chronic/long COVID-19 is crucial in understanding the management of long haulers of post COVID-19. Methods: Pub Med (Medline) database was scoped for original articles based on a search strategy related to the objectives. The selected articles post-screening were analyzed for variables relating to chronic/long COVID-19. Results were analyzed using descriptive statistics. Results: A total of 33 studies were reviewed. A total of 60% of the studies were observational studies and most of them were from high income countries. Almost half of the studies were in phase 3 of post-COVID-19, i.e., symptoms lasting >24 weeks. Among the physiological and psychological symptoms studied, fatigue, dyspnea, cough, headache, memory loss, depression, brain fog and lack of concentration were found to be the most frequently reported symptoms. Excessive sleep, constipation and neuropathic pain were among the least reported symptoms. Prior hospitalization, the female gender was found to be a risk factor. Limitations were reported by all studies. Conclusions: The major physiological and psychological symptoms of long COVID-19 have been explained with risk factors and its impact on patients’ lifestyles. The findings of this review hope to facilitate clinicians to draw conclusions to manage the long-term effects of post/chronic COVID-19.
Impact of Digital Device Use on Neck and Low Back Pain Intensity among Nursing Students at a Saudi Government University: A Cross-Sectional Study
Authors: Mahmoud Nermen A. , Abu Raddaha Ahmad H. , Zaghamir Donia E.
Published: December 2022

As digital technology and online activities have become more widely accessible over the past few years, information and communication technology have grown in importance in all students’ lives. Most of them routinely use digital devices for a range of activities, primarily for online learning, activities, assignments, conversing, and Internet browsing. This study elucidated the relationship between neck and low back pain intensity and the use of digital devices among Saudi nursing students. A cross-sectional descriptive exploratory research design was applied using a convenience sample of 120 nursing students enrolled in an undergraduate nursing program at a Saudi government university located in Riyadh, the capital city of Saudi Arabia. A valid and reliable self-administered survey was employed. Data about sociodemographic characteristics, the Neck Disability Index and the Roland–Morris Disability Questionnaire were collected. The participants reported 9.1 ± 4.6 study hours on average per week. A total of 82.5% of them do not receive enough exercise, and 87.5% indicated daily use of digital devices. Around half (54.2%) of participants reported having neck pain of a mild intensity. About 60% of the participants regularly shift positions to rest their backs, 39.2% are only able to stand for limited periods due to back issues, and 39.2% expressed concern for others about what might happen to their health. Although there was an association between neck pain intensity and the age or gender of all research participants, there was a significant association between neck pain severity and marital status (χ2 = 15.226, p = 0.019). Our findings suggest that nursing students should maintain neutral neck and back postures on a regular basis to reduce pain, which could be attributed to extensive reading on digital devices.
Comparison of Clinical Features, Complete Blood Count Parameters, and Outcomes between Two Distinct Waves of COVID-19: A Monocentric Report from Italy
Authors: Fois Sara Solveig , Zinellu Elisabetta , Zinellu Angelo , Merella Michela , Pau Maria Carmina , Carru Ciriaco , Fois Alessandro Giuseppe , Pirina Pietro
Published: December 2022

Background: Since the beginning of the SARS-CoV-2 pandemic, the ability to predict the trajectory of the disease has represented a major challenge for clinicians. There is recent evidence that complete blood cell count (CBC)-derived inflammation indexes have predictive value in COVID-19. We aimed to describe any changes in the clinical features, CBC-derived ratios, and outcomes of patients admitted to our hospital across two temporally distinct waves. Methods: We retrospectively assessed and compared the clinical characteristics and blood cell count values of patients hospitalized during the second and fourth waves of COVID-19, and explored any outcome differences in terms of the level of respiratory support required and transfer to intensive care. Results: We observed that fourth-wave patients were older, less male-predominant, and carried more comorbidities compared to the second-wave patients but, nevertheless, experienced more favorable outcomes. A strong internal correlation was documented for both waves between outcomes and CBC-derived ratios, with the fourth-wave cases displaying lower admission values of the neutrophil-to-lymphocyte ratio (NLR), derived NLR (dNLR), platelet-to-lymphocyte ratio (PLR), and systemic inflammation index (SII). No significant differences were found for lymphocyte-to-monocyte ratio (LMR), systemic inflammation response index (SIRI), and aggregate index of systemic inflammation (AISI). Conclusions: We observed that both admission values of CBC-derived indexes and adverse respiratory outcomes decreased from the second to the fourth wave of COVID-19. These data represent a contribution to the existing knowledge on the role of CBC-derived indexes as a potential tool to help clinicians to quickly differentiate in-hospital patients at increased risk of serious illness and death.
Combined Web-Based Visualisation of 3D Point Clouds and Acoustic Descriptors: An Interdisciplinary Challenge
Authors: Bergerot Laurent , Blaise Jean-Yves , Dudek Iwona , Pamart Anthony , Aramaki Mitsuko , Fargeot Simon , Kronland-Martinet Richard , Vidal Adrien , Ystad Sølvi
Published: December 2022

This paper presents an online application—called 3D integrator—enabling visual cross-examinations of architectural and acoustic data on web browsers and explains the rationale behind its development. The tool consists of a series of overlays developed over the Potree 3D point cloud renderer. It is used to display visually, in an interactive environment, the 3D point clouds resulting from a survey protocol tailored to the specific needs of the corpus under scrutiny—15 small-scale edifices often referred to as “minor heritage assets”—and to the research’s overall ambition, reasoning on architectural and acoustic features at an interdisciplinary level. One of the tool’s distinctive features is to project abstract information (acoustic descriptors) in a “close to real” 3D space (point clouds), hence merging scientific visualisation and information visualisation practices. The paper first shortly sums up the particularities of the survey protocol and discusses the implementation of the analytical add-ons that have been introduced (visual trace of the survey protocol itself, volume calculation, and exploratory 3D representation of acoustic descriptors). It then focuses on use cases that illustrate what the approach helps to observe concerning the interiors of edifices when capturing and co-examining dimensional and acoustic features.
Evaluation of the Acaricidal Activity of Lithium Chloride against Tetranychus urticae (Acari: Tetranychidae)
Authors: Solti Izabella , Kolics Éva , Keszthelyi Sándor , Bacsi Zsuzsanna , Staszny Ádám , Nagy Erzsébet , Taller János , Mátyás Kinga , Kolics Balázs
Published: December 2022

Tetranychus urticae is a severe threat and a major source of yield loss in some agricultural and horticultural crops and it is also as a vector for several viruses. The number of active substances used against the pest is limited. Therefore, there is a continuous need for new active substances. Recently, lithium salts have been shown to be one of the most promising potential alternatives to control Varroa destructor; an apicultural mite pest. Based on this, we aimed to test whether the efficacy of lithium chloride extends to other agricultural mite pests, such as the two-spotted spider mite. In the present pilot study, we report for the first time that the efficacy of lithium chloride is extended to the two-spotted spider mite. Additionally, this is the first report on the acaricidal effectiveness of lithium on a plant mite pest. In the present study, we report three different concentrations that bear 100% mortality at concentrations of 5.52 M, 2.76 M, and 1.38 M. The symptoms caused were similar and followed the same sequence compared to those observed on animal parasites such as V. destructor and Dermanyssus gallinae.
Overwintering Improves Ranunculus Cut Flower Production in the US Intermountain West
Authors: Rauter Shannon , Stock Melanie , Black Brent , Drost Dan , Dai Xin , Ward Ruby
Published: December 2022

Ranunculus (Ranunculus asiaticus L.) cut flower production in the US Intermountain West is limited by a narrow window of optimal temperature ranges in the spring. With the increasing number of Intermountain West cut flower growers, regional management recommendations are needed to improve ranunculus harvest timing and yield. The objectives of this research were to evaluate planting dates, winter insulation, tuberous root (TR) preparation, and cultivar selection for flower timing, yield, quality, and profitability in high tunnel and field production systems. Trials were conducted in a North Logan, UT (41.7665° N, −111.811° W, 1405 m elevation, USDA hardiness zone 5) high tunnel and field from fall 2019 to spring 2022. TRs were either pre-sprouted or directly planted into a high tunnel (left bare or covered with low tunnels) or field (left bare or covered with mulch, a low tunnel, or mulch and a low tunnel) from November to April. High tunnels advanced production by four weeks, nearly doubled total yield, and increased the proportion of quality (longer than 25 cm) stems by 50% compared to the field. Planting pre-sprouted TRs in the high tunnel in November delivered the earliest harvest (6 Apr.), highest marketable yield (286 stems per m2 ± 36 SE), and highest net returns ($54 per m2), with 39% greater marketable yield for ‘LaBelle’ than ‘Amandine.’ Insulation nearly doubled emergence and improved marketable yield by 49 stems per m2 ± 8 SE for November field plantings. Ranunculus production was optimized as a fall-planted high tunnel crop in the Intermountain West but may be fall-planted in the field with insulation, allowing growers to maximize production during more optimal, early-season temperatures.
Analysis of the Hydraulic Efficiency of a Steerable Detention Tank—Simulation Studies
Authors: Pochwat Kamil , Pizzo Henrique
Published: December 2022

The article contains the results of the first part of the research on the analysis of the operation of the retention device cooperating with the drainage system—steerable detention tank and concerns model simulation studies. The obtained results are an introduction to conducting laboratory tests. The planned research was carried out on the basis of the theory of experimental planning and hydrodynamic modelling, in which the systems of hydraulic parameters of the drainage system and hydrological of the catchment were selected. In total, over a thousand hydrodynamic simulations were carried out using SWMM 5.1. The planned analyses had two main goals. Firstly, to verify the possibility of reducing the rainwater volume flow in the drainage system by means of controllable devices enabling cooperation with the drainage system in various hydraulic conditions of the drainage system. Secondly, determining the impact of the connection method (parallel or serial) of the device enabling retention and cooperation with the sewage system on the efficiency of the system. The conducted analyses showed that the use of a retention device cooperating with the drainage system may significantly reduce the amount of sewage outfall from system, depending on the capacity of a single micro-accumulator, even up to 83% (in the analysed data range). It was also shown that the method of connecting the device to the network has an influence on the efficiency of the system in depend on hydraulic conditions.
Carbothermal Synthesis of Sludge Biochar Supported Nanoscale Zero-Valent Iron for the Removal of Cd2+ and Cu2+: Preparation, Performance, and Safety Risks
Authors: Shao Yingying , Tian Chao , Yang Yanfeng , Shao Yanqiu , Zhang Tao , Shi Xinhua , Zhang Weiyi , Zhu Ying
Published: December 2022

The practical application of nanoscale zero-valent iron (NZVI) is restricted by its easy oxidation and aggregation. Here, sludge biochar (SB) was used as a carrier to stabilize NZVI for Cd2+ and Cu2+ removal. SB supported NZVI (SB-NZVI) was synthesized using the carbothermic method. The superior preparation conditions, structural characteristics, and performance and mechanisms of the SB-NZVI composites for the removal of Cd2+ and Cu2+ were investigated via batch experiments and characterization analysis. The optimal removal capacities of 55.94 mg/g for Cd2+ and 97.68 mg/g for Cu2+ were achieved at a Fe/sludge mass ratio of 1:4 and pyrolysis temperature of 900 °C. Batch experiments showed that the SB-NZVI (1:4-900) composite had an excellent elimination capacity over a broad pH range, and that weakly acidic to neutral solutions were optimal for removal. The XPS results indicated that the Cd2+ removal was mainly dependent on the adsorption and precipitation/coprecipitation, while reduction and adsorption were the mechanisms that play a decisive role in Cu2+ removal. The presence of Cd2+ had an opposite effect on the Cu2+ removal. Moreover, the SB-NZVI composites made of municipal sludge greatly reduces the leaching toxicity and bio-availability of heavy metals in the municipal sludge, which can be identified as an environmentally-friendly material.
Mental Health of Cystic Fibrosis Patients and the COVID-19 Pandemic in Poland: A Single-Center Comparative Study
Authors: Humaj-Grysztar Magdalena , Rachel Marta , Śmiech-Michalec Olga , Bonior Joanna
Published: December 2022

Research shows that people with cystic fibrosis are more prone to suffer from psychological problems than healthy people; thus, the outbreak of the COVID-19 pandemic in Poland could have had an impact on their mental health. To assess this impact, we examined the mental health of patients before and during the pandemic. Survey participants were asked to fill in questionnaires that consisted of Beck Depression Inventory (BDI), 12-Item General Health Questionnaire (GHQ-12) and Cystic Fibrosis Questionnaire-Revised (CFQ-R; for the purpose of the study, an emotional functioning domain was used) during their hospital visits. A total of 81 patients took part in the study: 39 before the COVID-19 pandemic (BP) and 42 during the COVID-19 pandemic (DP). Patients’ medians were lower for the BDI, GHQ-12 and higher for the emotional domain of CFQ-R during the pandemic (3, 6, 75 vs. 4, 10, 73.33). Fewer patients felt that their mental health had deteriorated during the pandemic (Δχ2 = 7.723; p = 0.005), and GHQ-12 scores were lower in the DP group (Z = −3.044; p = 0.002). No significant differences were found between groups in terms of experiencing depressive symptoms (Δχ2 = 1.036; p = 0.309). It was found that patients with cystic fibrosis from our study group not only maintained but also improved their mental health state during the COVID-19 pandemic.
Development, Validation, and Utilization of a Social Media Use and Mental Health Questionnaire among Middle Eastern and Western Adults: A Pilot Study from the UAE
Authors: Hegazi Omar , Alalalmeh Samer , Alfaresi Ahmad , Dashtinezhad Soheil , Bahada Ahmed , Shahwan Moyad , Jairoun Ammar Abdulrahman , Babalola Tesleem K. , Yasin Haya
Published: December 2022

Objectives: We aimed to develop and validate a mental health stigma measurement tool for use within the social media context, utilizing the tool to assess whether the stigma shown in face-to-face interactions translates to social media, coupled with comparing whether social media use can cause the stigma among a sample of Middle Eastern and Western populations. Methods: The development and validation phase comprised a systematic process that was used to develop an assessment tool that could be used within the social media context and establish its validity and reliability. A 5-point Likert-type scale (1 = strongly disagree to 5 = strongly agree) was developed to assess mental health stigma. The anonymous questionnaire was distributed from June 2022 to August 2022 on various social media platforms and groups predominated by the two demographics of interest, enrolling 1328 participants (with only 1001 responses deemed valid). The utilization phase consisted of bivariate and multivariable analysis of the data. The cutoff points for low, medium, and high scores were the 25th, 50th, and 75th percentil, respectively. Results: The instrument comprised three dimensions: acceptance, intolerance, and digital care sentiment. In the Middle Eastern subset of participants, a higher score of intolerance (more stigma) toward mental illness was found in 72.4% of the participants, with a higher score of acceptance being 35.1% and of digital care sentiment being 46.4%. The mean scores for all the scales were as follows: intolerance (3.08 ± 0.64), acceptance (3.87 ± 0.71), and digital care sentiment (3.18 ± 0.69). For Westerners, a higher score of intolerance toward mental illness was found in 24.0% of the participants, with a higher score of acceptance being 56.8% and of digital care sentiment being 38.2%. The mean scores for all the scales were as follows: intolerance (2.28 ± 0.73), acceptance (4.21 ± 0.61), and digital care sentiment (3.08 ± 0.62). Various results were obtained regarding the effect of individual social media platforms on the different subscales. Conclusions: Stigma does follow people on social media, whether they are Middle Easterners or Westerners, although to varying degrees. The results of social media interaction and activity varied based on the group that used them, with some having an impact on one group but not the other. For these reasons, proper guidance is advised when utilizing and interacting with social media platforms.
Social Support and Symptoms of Depression in Late Life: Bidirectional Associations over Time
Authors: Turner Shira T. , Carmel Sara , O’Rourke Norm , Raveis Victoria H. , Tovel Hava , Cohn-Schwartz Ella
Published: December 2022

Social support functions as an effective buffer against depression, especially among older adults with limited social networks. For the current study, we examined longitudinal bidirectional associations between social support and depression among those 75+ years of age. We recruited and followed a sample of Israeli adults 75+ years of age (N = 824; M = 80.84; range 75–96 years). Structured interviews were conducted in the homes of participants at three annual points of measurement. Participants reported depressive symptoms and emotional and instrumental support received from friends and family. We examined a cross-lagged, longitudinal structural equation model (SEM) in which social support and depressive symptoms predict each other over time, covarying for previously reported social support and depressive symptoms. We found that both depressive symptoms and social support are largely consistent in late life. Depressive symptoms and social support reported at baseline predict levels reported 1 and 2 years thereafter. Cross-over effects emerged over time. Depressive symptoms predicted lower social support in future, and social support at baseline predicted depressive symptoms 2 years later. These findings suggest that associations between depressive symptoms and social support are bidirectional in late life. Further research is needed to replicate findings in other cultures and over longer periods, ideally until end of life.
Health Benefits of Residence at Moderate Altitude Do Not Reduce COVID-19 Mortality
Authors: Burtscher Johannes , Millet Gregoire P. , Leitner Barbara , Burtscher Martin
Published: December 2022

Moderate altitude (1000–2000 m above sea level) residence is emerging as a protective factor from the mortality of various causes, including of cardiovascular diseases. Conversely, mortality from certain respiratory diseases is higher at these altitudes than in lowlands. These divergent outcomes could indicate either beneficial or detrimental effects of altitude on the mortality of COVID-19 that primarily infects the respiratory tract but results in multi-organ damage. Previous epidemiological data indeed suggest divergent outcomes of moderate to high altitude residence in various countries. Confounding factors, such as variations in the access to clinical facilities or selection biases of investigated populations, may contribute to the equivocation of these observations. We interrogated a dataset of the complete population of an Alpine country in the center of Europe with relatively similar testing and clinical support conditions across altitude-levels of residence (up to around 2000 m) to assess altitude-dependent mortality from COVID-19 throughout 2020. While a reduced all-cause mortality was confirmed for people living higher than 1000 m, no differences in the mortality from COVID-19 between the lowest and the highest altitude regions were observed for the overall population and the population older than 60 years as well. Conversely, COVID-19 mortality seems to have been reduced in the very old (>85 years) women at moderate altitudes.
Using Tree-Based Machine Learning for Health Studies: Literature Review and Case Series
Authors: Hu Liangyuan , Li Lihua
Published: December 2022

Tree-based machine learning methods have gained traction in the statistical and data science fields. They have been shown to provide better solutions to various research questions than traditional analysis approaches. To encourage the uptake of tree-based methods in health research, we review the methodological fundamentals of three key tree-based machine learning methods: random forests, extreme gradient boosting and Bayesian additive regression trees. We further conduct a series of case studies to illustrate how these methods can be properly used to solve important health research problems in four domains: variable selection, estimation of causal effects, propensity score weighting and missing data. We exposit that the central idea of using ensemble tree methods for these research questions is accurate prediction via flexible modeling. We applied ensemble trees methods to select important predictors for the presence of postoperative respiratory complication among early stage lung cancer patients with resectable tumors. We then demonstrated how to use these methods to estimate the causal effects of popular surgical approaches on postoperative respiratory complications among lung cancer patients. Using the same data, we further implemented the methods to accurately estimate the inverse probability weights for a propensity score analysis of the comparative effectiveness of the surgical approaches. Finally, we demonstrated how random forests can be used to impute missing data using the Study of Women’s Health Across the Nation data set. To conclude, the tree-based methods are a flexible tool and should be properly used for health investigations.
Opposite Regulation of Homer Signal at the NMJ Postsynaptic Micro Domain between Slow- and Fast-Twitch Muscles in an Experimentally Induced Autoimmune Myasthenia Gravis (EAMG) Mouse Model
Authors: Schubert Martin , Pelz Andreas , Trautmann Gabor , Block Katharina , Furlan Sandra , Gutsmann Martina , Kohler Siegfried , Volpe Pompeo , Blottner Dieter , Meisel Andreas , Salanova Michele
Published: December 2022

Accelerated postsynaptic remodelling and disturbance of neuromuscular transmission are common features of autoimmune neurodegenerative diseases. Homer protein isoform expression, crosslinking activity and neuromuscular subcellular localisation are studied in mouse hind limb muscles of an experimentally induced autoimmune model of Myasthenia Gravis (EAMG) and correlated to motor end plate integrity. Soleus (SOL), extensor digitorum longus (EDL) and gastrocnemius (GAS) skeletal muscles are investigated. nAChR membrane clusters were studied to monitor neuromuscular junction (NMJ) integrity. Fibre-type cross-sectional area (CSA) analysis is carried out in order to determine the extent of muscle atrophy. Our findings clearly showed that crosslinking activity of Homer long forms (Homer 1b/c and Homer2a/b) are decreased in slow-twitch and increased in fast-twitch muscle of EAMG whereas the short form of Homer that disrupts Homer crosslinking (Homer1a) is upregulated in slow-twitch muscle only. Densitometry analysis showed a 125% increase in Homer protein expression in EDL, and a 45% decrease in SOL of EAMG mice. In contrast, nAChR fluorescence pixel intensity decreased in endplates of EAMG mice, more distinct in type-I dominant SOL muscle. Morphometric CSA of EAMG vs. control (CTR) revealed a significant reduction in EDL but not in GAS and SOL. Taken together, these results indicate that postsynaptic Homer signalling is impaired in slow-twitch SOL muscle from EAMG mice and provide compelling evidence suggesting a functional coupling between Homer and nAChR, underscoring the key role of Homer in skeletal muscle neurophysiology.
A Novel bHLH Transcription Factor PtrbHLH66 from Trifoliate Orange Positively Regulates Plant Drought Tolerance by Mediating Root Growth and ROS Scavenging
Authors: Liang Beibei , Wan Shiguo , Ma Qingling , Yang Li , Hu Wei , Kuang Liuqing , Xie Jingheng , Huang Yingjie , Liu Dechun , Liu Yong
Published: December 2022

Drought limits citrus yield and fruit quality worldwide. The basic helix-loop-helix (bHLH) transcription factors (TFs) are involved in plant response to drought stress. However, few bHLH TFs related to drought response have been functionally characterized in citrus. In this study, a bHLH family gene, named PtrbHLH66, was cloned from trifoliate orange. PtrbHLH66 contained a highly conserved bHLH domain and was clustered closely with bHLH66 homologs from other plant species. PtrbHLH66 was localized to the nucleus and had transcriptional activation activity. The expression of PtrbHLH66 was significantly induced by polyethylene glycol 6000 (PEG6000) and abscisic acid (ABA) treatments. Ectopic expression of PtrbHLH66 promoted the seed germination and root growth, increased the proline and ABA contents and the activities of antioxidant enzymes, but reduced the accumulation of malondialdehyde (MDA) and reactive oxygen species (ROS) under drought stress, resulting in enhanced drought tolerance of transgenic Arabidopsis. In contrast, silencing the PtrbHLH66 homolog in lemon plants showed the opposite effects. Furthermore, under drought stress, the transcript levels of 15 genes involved in ABA biosynthesis, proline biosynthesis, ROS scavenging and drought response were obviously upregulated in PtrbHLH66 ectopic-expressing Arabidopsis but downregulated in PtrbHLH66 homolog silencing lemon. Thus, our results suggested that PtrbHLH66 acted as a positive regulator of plant drought resistance by regulating root growth and ROS scavenging.
Kaempferol: Antimicrobial Properties, Sources, Clinical, and Traditional Applications
Authors: Periferakis Argyrios , Periferakis Konstantinos , Badarau Ioana Anca , Petran Elena Madalina , Popa Delia Codruta , Caruntu Ana , Costache Raluca Simona , Scheau Cristian , Caruntu Constantin , Costache Daniel Octavian
Published: December 2022

Flavonoids are a category of plant-derived compounds which exhibit a large number of health-related effects. One of the most well-known and studied flavonoids is kaempferol, which can be found in a wide variety of herbs and plant families. Apart from their anticarcinogenic and anti-inflammatory effects, kaempferol and its associated compounds also exhibit antibacterial, antifungal, and antiprotozoal activities. The development of drugs and treatment schemes based on these compounds is becoming increasingly important in the face of emerging resistance of numerous pathogens as well as complex molecular interactions between various drug therapies. In addition, many of the kaempferol-containing plants are used in traditional systems all over the world for centuries to treat numerous conditions. Due to its variety of sources and associated compounds, some molecular mechanisms of kaempferol antimicrobial activity are well known while others are still under analysis. This paper thoroughly documents the vegetal and food sources of kaempferol as well as the most recent and significant studies regarding its antimicrobial applications.
Non-Small Cell Lung Cancer Targeted Therapy: Drugs and Mechanisms of Drug Resistance
Authors: Wu Jiajia , Lin Zhenghong
Published: December 2022

The advent of precision medicine has brought light to the treatment of non-small cell lung cancer (NSCLC), expanding the options for patients with advanced NSCLC by targeting therapy through genetic and epigenetic cues. Tumor driver genes in NSCLC patients have been uncovered one by one, including epidermal growth factor receptor (EGFR), mesenchymal lymphoma kinase (ALK), and receptor tyrosine kinase ROS proto-oncogene 1 (ROS1) mutants. Antibodies and inhibitors that target the critical gene-mediated signaling pathways that regulate tumor growth and development are anticipated to increase patient survival and quality of life. Targeted drugs continue to emerge, with as many as two dozen approved by the FDA, and chemotherapy and targeted therapy have significantly improved patient prognosis. However, resistance due to cancer drivers’ genetic alterations has given rise to significant challenges in treating patients with metastatic NSCLC. Here, we summarized the main targeted therapeutic sites of NSCLC drugs and discussed their resistance mechanisms, aiming to provide new ideas for follow-up research and clues for the improvement of targeted drugs.
Phosphorylation of CaMK and CREB-Mediated Cardiac Aldosterone Synthesis Induced by Arginine Vasopressin in Rats with Myocardial Infarction
Authors: Zhai Yuan-Sheng , Li Jie , Peng Longyun , Lu Guihua , Gao Xiuren
Published: December 2022

Both aldosterone and arginine vasopressin (AVP) are produced in the heart and may participate in cardiac fibrosis. However, their relationship remains unknown. This study aims to demonstrate the regulation and role of AVP in aldosterone synthesis in the heart. Rats were subjected to a sham operation or myocardial infarction (MI) by ligating the coronary artery. Cardiac function and fibrosis were assessed using echocardiography and immunohistochemical staining, respectively. In addition, the effects of AVP stimulation on cardiac microvascular endothelial cells (CMECs) were studied using ELISA, real-time PCR, and Western blotting. Compared with the rats having undergone a sham operation, the MI rats had an increased LVMI, type I collagen composition, and concentrations of aldosterone and AVP in the heart but decreased cardiac function. As the MI rats aged, the LVMI, type I collagen, aldosterone, and AVP increased, while the LVMI decreased. Furthermore, AVP time-dependently induced aldosterone secretion and CYP11B2 mRNA expression in CMECs. The p-CREB levels were significantly increased by AVP. Nevertheless, these effects were completely blocked by SR49059 or partially inhibited by KN93. This study demonstrated that AVP could induce the secretion of local cardiac aldosterone, which may involve CaMK and CREB phosphorylation and CYP11B2 upregulation through V1 receptor activation.
UCP2 as a Cancer Target through Energy Metabolism and Oxidative Stress Control
Authors: Luby Angèle , Alves-Guerra Marie-Clotilde
Published: December 2022

Despite numerous therapies, cancer remains one of the leading causes of death worldwide due to the lack of markers for early detection and response to treatment in many patients. Technological advances in tumor screening and renewed interest in energy metabolism have allowed us to identify new cellular players in order to develop personalized treatments. Among the metabolic actors, the mitochondrial transporter uncoupling protein 2 (UCP2), whose expression is increased in many cancers, has been identified as an interesting target in tumor metabolic reprogramming. Over the past decade, a better understanding of its biochemical and physiological functions has established a role for UCP2 in (1) protecting cells from oxidative stress, (2) regulating tumor progression through changes in glycolytic, oxidative and calcium metabolism, and (3) increasing antitumor immunity in the tumor microenvironment to limit cancer development. With these pleiotropic roles, UCP2 can be considered as a potential tumor biomarker that may be interesting to target positively or negatively, depending on the type, metabolic status and stage of tumors, in combination with conventional chemotherapy or immunotherapy to control tumor development and increase response to treatment. This review provides an overview of the latest published science linking mitochondrial UCP2 activity to the tumor context.
Rapid Detection of Brettanomyces bruxellensis in Wine by Polychromatic Flow Cytometry
Authors: De Bellis Domenico , Di Stefano Alessio , Simeone Pasquale , Catitti Giulia , Vespa Simone , Patruno Antonia , Marchisio Marco , Mari Eleonora , Granchi Lisa , Viti Carlo , Chiacchiaretta Piero , Cichelli Angelo , Tofalo Rosanna , Lanuti Paola
Published: December 2022

Brettanomyces bruxellensis is found in several fermented matrices and produces relevant alterations to the wine quality. The methods usually used to identify B. bruxellensis contamination are based on conventional microbiological techniques that require long procedures (15 days), causing the yeast to spread in the meantime. Recently, a flow cytometry kit for the rapid detection (1–2 h) of B. bruxellensis in wine has been developed. The feasibility of the method was assessed in a synthetic medium as well as in wine samples by detecting B. bruxellensis in the presence of other yeast species (Saccharomyces cerevisiae and Pichia spp.) and at the concentrations that produce natural contaminations (up to 105 cells/mL), as well as at lower concentrations (103–102 cells/mL). Wine samples naturally contaminated by B. bruxellensis or inoculated with four different strains of B. bruxellensis species together with Saccharomyces cerevisiae and Pichia spp., were analyzed by flow cytometry. Plate counts were carried out in parallel to flow cytometry. We provide evidence that flow cytometry allows the rapid detection of B. bruxellensis in simple and complex mixtures. Therefore, this technique has great potential for the detection of B. bruxellensis and could allow preventive actions to reduce wine spoilage.
Proteomic Signature and mRNA Expression in Hippocampus of SAMP8 and SAMR1 Mice during Aging
Authors: Reale Marcella , Costantini Erica , Aielli Lisa , Di Giuseppe Fabrizio , Angelucci Stefania , Kamal Mohammad A. , Greig Nigel H.
Published: December 2022

Aging is a complex process often accompanied by cognitive decline that represents a risk factor for many neurodegenerative disorders including Alzheimer’s and Parkinson’s disease. The molecular mechanisms involved in age-related cognitive decline are not yet fully understood, although increased neuroinflammation is considered to play a significant role. In this study, we characterized a proteomic view of the hippocampus of the senescence-accelerated mouse prone-8 (SAMP8), a model of enhanced senescence, in comparison with the senescence-accelerated-resistant mouse (SAMR1), a model of normal aging. We additionally investigated inflammatory cytokines and cholinergic components gene expression during aging in the mouse brain tissues. Proteomic data defined the expression of key proteins involved in metabolic and cellular processes in neuronal and glial cells of the hippocampus. Gene Ontology revealed that most of the differentially expressed proteins are involved in the cytoskeleton and cell motility regulation. Molecular analysis results showed that both inflammatory cytokines and cholinergic components are differentially expressed during aging, with a downward trend of cholinergic receptors and esterase enzymes expression, in contrast to an upward trend of inflammatory cytokines in the hippocampus of SAMP8. Together, our results support the important role of the cholinergic and cytokine systems in the aging of the murine brain.
Trigeminal Sensory Supply Is Essential for Motor Recovery after Facial Nerve Injury
Authors: Rink-Notzon Svenja , Reuscher Jannika , Nohroudi Klaus , Manthou Marilena , Gordon Tessa , Angelov Doychin N.
Published: December 2022

Recovery of mimic function after facial nerve transection is poor. The successful regrowth of regenerating motor nerve fibers to reinnervate their targets is compromised by (i) poor axonal navigation and excessive collateral branching, (ii) abnormal exchange of nerve impulses between adjacent regrowing axons, namely axonal crosstalk, and (iii) insufficient synaptic input to the axotomized facial motoneurons. As a result, axotomized motoneurons become hyperexcitable but unable to discharge. We review our findings, which have addressed the poor return of mimic function after facial nerve injuries, by testing the hypothesized detrimental component, and we propose that intensifying the trigeminal sensory input to axotomized and electrophysiologically silent facial motoneurons improves the specificity of the reinnervation of appropriate targets. We compared behavioral, functional, and morphological parameters after single reconstructive surgery of the facial nerve (or its buccal branch) with those obtained after identical facial nerve surgery, but combined with direct or indirect stimulation of the ipsilateral infraorbital nerve. We found that both methods of trigeminal sensory stimulation, i.e., stimulation of the vibrissal hairs and manual stimulation of the whisker pad, were beneficial for the outcome through improvement of the quality of target reinnervation and recovery of vibrissal motor performance.
Technical Study of Automated High-Throughput High-Sensitive Ceruloplasmin Assay on Dried Blood Spots—Reinstate the Potential Use for Newborn Screening of Wilson Disease
Authors: Mak Chloe Miu , Choi Ching Tung , Wong Tsz Ki , Chin Hanson Heearn , Lai Hillman Kai Yin , Yuet Koon Yu
Published: December 2022

In this study, we modified a fully automatic immunoassay on ceruloplasmin concentration on dried blood spots (DBS) to increase its analytical sensitivity in order to accurately differentiate newborns from true Wilson disease (WD) patients. Modifications to the assay parameters of the Roche/Hitachi Cobas c systems immunoturbidimetric assay are adjusted to lower the limit of quantitation to 0.60 mg/L from 30 mg/L. This enables sensitive measurement of ceruloplasmin in eluent after DBS extraction. In addition, reference intervals and receiver operating characteristic curve analysis for diagnostic cut-off were established using DBS of neonates and WD adult patients. After DBS whole blood calibration, the 95th percentile of the reference interval for newborns was 86–229 mg/L. The cut-off value of 54 mg/L was found to be the most optimal point for differentiating true adult WD from newborn controls. This test shows a high area under curve of 1.000 with 100% sensitivity and specificity in differentiating normal newborns from WD adult samples. However, the results should be further validated with true newborn WD patient samples together with the consideration of other factors that can also lead to low ceruloplasmin levels. This test shows application potential in newborn screening for WD, which can save lives through early identification and timely treatment.
Effects of Thymoquinone Alone or in Combination with Losartan on the Cardiotoxicity Caused by Oxidative Stress and Inflammation in Hypercholesterolemia
Authors: Al-Oanzi Ziad H. , Alenazy Fawaz O. , Alhassan Hassan H. , El-Aassar Mohamed R. , Alzarea Abdulaziz I. , Alzarea Sami I. , Abbas Anass M. , Alanazi Muteb H. , Al-Enazi Maher M.
Published: December 2022

Dietary cholesterol accelerates oxidative and pro-inflammatory processes, causing hypercholesterolemia and cardiovascular diseases. Thus, the purpose of the current study is to compare the protective effects of thymoquinone (TQ) alone or in combination with losartan (LT) against the heart damage caused by a high-cholesterol diet (HCD). HCD-fed rat groups revealed an elevated activity of indicators of cardiac enzymes in the serum. Serum and cardiac lipids were also found to be significantly higher in HCD-fed rat groups. Cardiac pro-inflammatory and oxidative markers were also increased in HCD-fed rat groups, whereas antioxidant indicators were decreased. However, all of these biochemical, inflammatory, antioxidant, and oxidative change indicators returned to levels similar to those of normal rats after treatment with TQ alone or in combination with LT administered to HCD-fed rat groups. Hypercholesterolemia considerably induced the lipid peroxidation product, thiobarbituric acid reaction substances (TBARs), and oxidative radicals in cardiac cells, which were attenuated by QT and LT treatments, particularly when combined. Finally, QT, LT, and their combination were able to reduce the histological changes changes brought on by cholesterol excess in cardiac tissues. In conclusion, administration of TQ in a combination with LT which has a better protective effect, significantly reduced the hypercholesterolemic-induced oxidative and inflammatory changes that occurred in cardiac tissue.