bluuebunny/crossref_metadata_2025 huggingface dataset:
The Perception of Indonesian Nursing Students on the Learning Environment in Clinical Practice
Authors: Nahariani Pepin , Kurdi Fahruddin , Priyanti Ratna Puji
Published: June 2019

Introduction: The clinical practice environment students have multiple roles as learners and service providers. At this time students are faced with a fluctuating, complex environment and far from the predictions and controls of academic teachers. The aim of this study was to evaluate nursing student satisfaction and perception of the learning environment in clinical practice.Methods: This study used correlational analytic survey methods with cross-sectional approach. A total of 164 nursing student were selected using a convenience sampling. The data were collected in three schools of nursing in East Java. Data were obtained using the Bahasa version of the Clinical Learning Environment, Supervisions and Nurse Teacher (CLES+T). The instrument showed good validity and reliability. The data were analyzed using descriptive analytic, Spearman rho correlation and logistic regression.Results: Respondents were 164 students, clinical learning practice is mainly determined by the role of nurse teacher (M = 3.82; SD = 0.496). Student satisfaction obtained at 56.7%. There was a significant relationship between clinical practice learning and student satisfaction with p-value 0.000. The highest correlation value is the role of lecturer nurse (r = 0.544), with value R2 = 0.377, the coefficient of β adjusted highest to 2.075.Conclusion: According to student perceptions, the content of supervisory relations, the pedagogy atmosphere in the ward/room and the role of the nurse lecturer contributed greatly to creating a conducive clinical learning environment.
G20 ÜLKELERİNİN EKONOMİK PERFORMANSLARININ GRİ İLİŞKİSEL ANALİZ YÖNTEMİ İLE DEĞERLENDİRİLMESİ - ASSESSING THE ECONOMIC PERFORMANCE OF THE G20 MEMBERS BY USING GREY RELATIONAL ANALYSIS
Authors: BÜYÜKGEBİZ KOCA Eda , TUNCA Mustafa Zihni
Published: June 2019

Dünya çapında geçmişten günümüze pek çok ekonomik, siyasi, toplumsal kriz yaşanmıştır. Her ne kadar bu krizler önemli ölçüde ülkesel ya da bölgesel düzeyde gerçekleşse de Büyük Buhran ve Küresel Finans Krizi gibi bazı ekonomik krizler tüm dünyayı önemli ölçüde etkilemiştir.  Bu çalışmada G20 ülkelerinin Küresel Finans Krizi’nden etkilenme durumları çok kriterli karar verme tekniklerinden biri olan Gri İlişkisel Analiz yöntemiyle incelenmiştir. Bu doğrultuda G20 ülkelerine ait çeşitli makro ekonomik veriler 2000 ve 2017 yıllarını da kapsayacak şekilde kriz öncesi dönem, kriz dönemi ve kriz sonrası dönem olmak üzere altışar yıllık üç dönem halinde ele alınmıştır. Çalışma sonucunda çok sayıda ülkenin Küresel Finans Krizi’nden olumsuz etkilendiği gözlenmiştir.
Weak and Strong Warm Logamediate Anisotropic Inflationary Universe Model
Authors: Javed Wajiha , Nawazish Iqra , Khan Mohsin Raza , Övgün Ali
Published: June 2019

This paper is given to the investigation of warm inflation using Modified Chaplygin gas in the background of locally rotationally symmetric Bianchi Identity type I. We find out the field equations and perturbations parameters such as; scalar power spectrum, scalar spectral index, scalar potential and tensor to scalar ratio under slow roll approximation. We find out these parameters in directional of Hubble parameter during the Logamediate inflationary regime in weak and strong case. These comological parameters shows that the anisotropic model is also compatible WMAP$7$ with recent observational data Planck $2018$.
Formation of Nanoporous Mixed Aluminum-Iron Oxides by Self-Organized Anodizing of FeAl3 Intermetallic Alloy
Authors: Chilimoniuk Paulina , Michalska-Domańska Marta , Czujko Tomasz
Published: June 2019

Nanostructured anodic oxide layers on an FeAl3 intermetallic alloy were prepared by two-step anodization in 20 wt.% H2SO4 at 0°C. The voltage range was 10.0 – 22.5 V with a step of 2.5 V. The structural and morphological characterizations of the received anodic oxide layers were performed by FE-SEM. Therefore, the formed anodic oxide was found to be highly porous with a high surface area, as indicated by the FE-SEM studies. It has been shown that the morphology of fabricated nanoporous oxide layers is strongly affected by the anodization potential. The oxide growth rate first increased slowly (from 0.010 μm/s for 10 V to 0.02 μm/s for 15 V) and then very rapidly (from 0.04 μm/s for 17.5 V up to 0.13 μm/s for 22.5 V). The same trend was observed for the change in the oxide thickness. Moreover, for all investigated anodizing voltages, the structural features of the anodic oxide layers, such as the pore diameter and interpore distance, increase with increasing anodizing potential. The obtained anodic oxide layer was identified as a crystalline FeAl2O4, Fe2O3 and Al2O3 oxide mixture.
Surface Micromorphology and Crystalline Structures of Hard Dental Tissue Treated by Atmospheric Pressure Plasma Jet
Authors: Beryani Nezafat Negin , Kulesza Slawomir , Ghoranneviss Mahmood , Elahi Seyed Mohammad , Bramowicz Miroslaw , Shafiekhani Azizollah , Ţălu Ştefan , Ghorannevis Zohreh , Solaymani Shahram
Published: June 2019

In this paper, the results of helium plasma jet treatment of hard tissues of natural human teeth including enamel, dentin, and cementum are presented. Changes in surface morphology and structural properties have been investigated using scanning electron microscopy (SEM) in connection with multiscale image analysis, X-ray diffraction (XRD) with the aid of the Rietveld method and Williamson-Hall plots, energy dispersive X-ray (EDX) and Raman spectra analysis. Despite initial differences in scaling behaviors of the surfaces of freshly extracted tissues, spatial characteristics of their plasma-treated counterparts appeared similar taking into account the fractal measures. On the other hand, despite improvement in crystalline structure of enamel due to the plasma treatments, the structure of cementum has been found intact.
The Effectiveness of Progressive and Traditional Coaching Strategies to Improve Sprint and Jump Performance across Varying Levels of Maturation within a General Youth Population
Authors: Standing Regan , Maulder Peter
Published: June 2019

Literature pertaining to youth development has identified the importance of understanding the physical, intellectual and emotional needs of adolescent youth. The purpose of this study was to compare the use of a ‘traditional’ and ‘progressive’ coaching style to train a general male youth population to improve sprint and jump performances, whilst assessing enjoyment to comment on long term application. Maximal sprint times, sprint kinematics, unilateral jump distances and repetitive tuck jump scores were measured alongside anthropometric variables to characterise performance. Results revealed significant (p>0.05) pre/post differences in anthropometric variables across all maturation groups, and each of the maturational levels displayed a tendency to favour a particular coaching or control condition. Pre-PHV groups responded most effectively to the progressive style of coaching, displaying improvements in horizontal jump performances, and -0.7 to -2.7% improvements in all sprint times, despite also showing the largest increase in tuck jump scores (25.8%). The circa-PHV group produced their greatest improvements in the traditional intervention, as displayed through significant improvements (p<0.05) in 20m sprint times and dominant-leg horizontal jump performance, whilst also revealing the greatest deterioration in tuck jump scores (14.2%). Post-PHV displayed the greatest improvements in the control setting, suggesting the natural benefits gained through adolescent development were greater than the influence of the training interventions. In conclusion, it is suggested that matching coaching strategies and delivery techniques to the period of biological maturation may have implications for both performance and athlete safety.
“I’ve only just heard about it”: Complementary and Alternative Medicine Knowledge and Educational Needs of Clinical Psychologists in Indonesia
Authors: Liem Andrian
Published: June 2019

Background and objectives: The inadequate knowledge of complementary and alternative medicine (CAM) among health professionals may put their clients in risky situation because they then would find information about CAM from unreliable sources. Clinical psychologists (CPs), as health professionals, have also the opportunity to provide psychoeducation on the latest CAM scientific research to their clients. The current study aimed to explore knowledge and educational needs of CAM among CPs in Indonesia because previous studies on exploring CAM knowledge and educational needs of CAM were primarily conducted in Western countries. Materials and Methods: Data were collected through semi-structured face-to-face interviews with 43 CPs in public health centers (PHCs) in Indonesia. Most interviews were held at the PHCs where participants worked and interviews lasted for 55 minutes, on average. The interview recordings were transcribed and were analyzed using deductive thematic analysis. Results: Five main themes emerged within participants’ responses regarding CAM knowledge and educational needs. First (CAM understanding), participants’ responses ranged from those with little or no prior knowledge of CAM treatments and uses, to those with much greater familiarity. Second (source of knowledge), participants’ access ranged widely in terms of references, from popular to scientific literature. Third (why is it important?), participants identified CAM essentially as part of Indonesian culture and it was therefore crucial to have this cultural knowledge. Fourth (the challenges and what is needed?), the challenges for improving participants’ knowledge came from personal and institutional levels. Fifth (what and how to learn?), participants advised that only CAM treatments that fit in brief psychotherapy sessions should be introduced in professional training. Conclusions: This qualitative study discovered that CAM was neither well-known nor understood widely. Participants advised that professional associations and health institutions should work together in enhancing knowledge of CAM and incorporating CAM education into psychology education.
Understanding Substance Abuse and its Correlation with Social Changes in Sikkim: A Sociological Study
Authors: Rai Peter
Published: June 2019

In contemporary society, the full-fledged impact of modernisation and globalisation which has given the free movement of people, goods and money across the countries of the world can be witnessed. This has also opened the economic opportunities among the individuals and communities in the society, which has become a new way of life. Society has changed over the years and activities of the people have also undergone social change. Even the trend of taking drugs has changed with change in society. Traditionally used drugs have been replaced by modern synthetic drugs. The proliferation of pharmaceutical industries, which manufactures narcotic drugs, has, directly and indirectly, encouraged the use and abuse of drugs due to easy access in the market. The epidemic of illicit drug users in the global society has increased significantly and simultaneously related crimes in society. 

Drug abuse is global phenomena. A drug is a biological substance, synthetic or natural, which is taken primarily for non-dietary needs, and it is a substance, which affects the functioning of the mind and body or both. Globally, according to UNODC estimate, in 2009, between 149 and 272 million people, or 3.3% to 6.1% of the population aged 15-64, had used illicit substance once in the previous year. Cannabis and ATS (Amphetamine-type stimulants) are two important drugs which are commonly used worldwide. Within Asia, ATS ranks as the main drug of abuse in Thailand, Japan, Republic of Korea and the Philippines, and also China, Myanmar and Indonesia are in the second rank (UNODC, 2004). Heroin, cocaine and other drugs kill around 0.2 million people each year and also causes health problems with incurable diseases. The European Monitoring Centre for Drug and Drug Addiction (EMCDDA) defines the problem of drug use as, “injecting drug use or long duration regular use of opium, cocaine and amphetamines”. World Health Organisation (WHO), defines drug addiction as a ‘disease,’ and the American Psychiatric Association, define drug abuse as the ‘illicit consumption of any naturally occurring of pharmaceutical substance for the purpose of changing the way, in which a person feels, thinks or behaves, without understanding or taking into consideration the damaging physical and mental side-effects that are caused.'
KINERJA KEUANGAN SESUDAH DAN SEBELUM SPIN OFF UNIT USAHA SYARIAH KE BANK UMUM SYARIAH
Authors: Kuncoro Amin , Yulianto Heru
Published: June 2019

 
This study aims to distinguish between conventional banks and Islamic banks before and after Spin Off. The population and samples are Islamic banks in 2011 - 2013 before spin off and 2013 - 2016 after the spin off totaling 5 banks. The design of this study uses descriptive analytics, with the results of the study there are differences in Islamic banks that do spin offs.
Editorial
Authors: Oliver Beverley
Published: June 2019

This is a momentous year for the Journal of Teaching and Learning for Graduate Employability – having started from scratch in 2010, this journal was established as an outcome of Curtin University’s ‘Curriculum 2010’ curriculum renewal initiative, particularly to create a forum where teaching academics might engage in scholarly reflection and research associated with graduate employability, a relatively new field in Australia at that time. In 2010, we welcomed contributions about any aspect of higher education teaching and learning which related to the broad topic of graduate employability, including but not limited to:Graduate employability and how it can be measured;The relationship between graduate employability and the achievement of graduate attributes, employability skills and preparation for global citizenship;Teaching and learning experiences, resources and assessments which enhance graduate employability;Industry partnerships and perspectives;Life-long learning.As we go forward, it is appropriate to note our achievements to date: this year, the journal will have been publishing for ten consecutive years: we published sixty-four peer-reviewed articles by May 2019, and ten editorials, and we have just published our first special issue (with plans for more). We are all interested not just in publications, but their impact. Citations clearly take time to build so focussing on our first six years (2010 to 2016) our thirty-three peer-reviewed articles were cited (in total) 570 times, and five articles were cited more than 40 times (to May 2019). Our most highly cited article to date – with 78 citations – is Catherine Caballero and Arlene Walker’s “Work readiness in graduate recruitment and selection: A review of current assessment methods”.
The Role of Advanced MRI in the Development of Treat-to-Target Therapeutic Strategies, Patient Stratification and Phenotyping in Rheumatoid Arthritis
Authors: Mobasheri Ali , Hinton Mark , Kubassova Olga
Published: June 2019

In this commentary we discuss the potential of advanced imaging, particularly Dynamic Contrast Enhanced (DCE) magnetic resonance imaging (MRI) for the objective assessment of disease progression in rheumatoid arthritis (RA). We emphasise the potential DCE-MRI in advancing the field and exploring new areas of research and development in RA. We believe that different grades of bone marrow edema (BME) and synovitis in RA can be examined and monitored in a more sensitive manner with DCE-MRI. Future treatments for RA will be significantly improved by enhanced imaging of BMEs and synovitis. DCE-MRI will also facilitate enhanced stratification and phenotyping of patients enrolled in clinical trials.
Health professionals’ perceptions of colorectal cancer patients’ treatment burden and their supportive work to ameliorate the burden - a qualitative study
Authors: Husebø Anne Marie Lunde , Karlsen Bjørg , Husebø Sissel I. Eikeland
Published: June 2019

Background

Support is pivotal for patients in managing colorectal cancer treatment, as they might be overwhelmed by the burden of treatment. There is scarce knowledge regarding health professionals’ perceptions of colorectal cancer patients’ burden in the treatment trajectory during the hospital stay and their views on supportive factors that may ameliorate the burden.

Aim

The aim of the study was to describe how health professionals perceive aspects of the treatment burden among patients receiving curative surgical treatment for colorectal cancer during the hospital stay and how they support patients to ameliorate the burden.

Methods

This study has an explorative, qualitative design, using semi-structured interviews with nine health professionals recruited from a university hospital. Data were systematised and coded by systematic text condensation.Results: Data analysis identified the themes “capturing patients’ burdens of colorectal cancer treatment” and “health professionals’ support to ameliorate the burden”. Patients with colorectal cancer had to face burdens related to a challenging emotional situation, treatment complications and side effects, and an extensive need for information. A trusting patient-carer relationship was therefore perceived as the essence of health professionals’ support. Health professionals focused their support on safeguarding patients, motivating patients to self-manage, and involving family and peers as supporters. Patients’ journey characteristics and illness severity challenged health professionals’ supportive work.

Conclusion

Support from health professionals includes providing patients emotional support and relevant treatment-related information and motivating patients for early post-surgical mobilisation. Health professionals should thus be aware of identifying colorectal cancer patients’ information needs according to the specific treatment stages, which may ameliorate the burden of colorectal cancer treatment and enable patients to self-manage at home.
Transcriptome analysis provides insights into key gene(s) involved in steroidal alkaloid biosynthesis in the medicinally important herb Fritillaria taipaiensis
Authors: Wang Xiaoyue , Luo Hong-mei , Wei Xue-min , Cao Pei , Gao Zi-tong , Han Jian-ping
Published: June 2019

Background

Fritillariae Cirrhosae Bulbus, which is a traditional antitussive and expectorant medicine in China, is derived from six original plants. Among these six species, all except for Fritillaria taipaiensis and Fritillaria unibracteata var. wabuensis have been recorded in the National Protected Plants III. Their major bioactive compounds, including imperialine and peimisine, are steroidal alkaloids, derived from the steroid synthesis pathway. However, research on the F. taipaiensis genome, transcriptomes and steroid-synthesis-related genes has been lacking for a long time. In this study, the first transcriptome sequencing of F. taipaiensis was performed.

Results

A total of 94,396,694 (~11.4 Gb) high-quality reads obtained using paired-end Illumina sequencing were de novo assembled into 190,350 transcripts. Functional annotation with multiple public databases identified an array of genes involved in steroidal alkaloid biosynthesis and other secondary metabolite pathways, including steroid and terpenoid backbone biosynthesis, and important oxidoreductases. The large number of differentially expressed transcripts together with CYPs suggests the involvement of these candidates in tissue-specific expression. The expression analysis revealed that bulbs may be the main site of the upstream steroidal alkaloid biosynthesis pathway, and we speculated that the downstream reactions, including the oxidation-reduction reaction catalyzed by CYPs, might also occur primarily in bulbs.

Conclusion

In conclusion, the comprehensive transcriptome dataset created in this study will serve as a resource for the identification of potential candidates for the genetic manipulation of targeted bioactive metabolites and will also contribute to the development of functionally relevant molecular marker resources to expedite molecular breeding and conservation efforts for F. taipaiensis.
One-step dual CRISPR/Cas9 guide RNA cloning protocol
Authors: Beucher Anthony , Cebola Ines
Published: June 2019

Existing protocols for dual guide RNA cloning rely on synthesised DNA oligonucleotides of >100 bp that contain both guide RNA sequences, and are therefore not reusable in alternative experimental designs. Here, we describe a single-step protocol to rapidly and inexpensively generate vectors expressing two guide RNAs (gRNAs) simultaneously, which allows re-usage of gRNAs oligonucleotides from one experimental design to another. This protocol is applicable to cloning gRNAs into virtually any CRISPR/Cas9 backbone that allows cloning by Golden Gate, by adapting the primer design. Here, we provide details for cloning gRNAs into vectors with BbsI and BsmBI sites, two of the most frequently found enzymes in CRISPR/Cas9 gRNA expression cassettes.This protocol has been successfully applied to delete pancreatic islet enhancers that harbour type 2 diabetes variants and to validate enhancer-promoter interactions (Miguel-Escalada et al., Nature Genetics 2019).In the future, we foresee that this simple protocol may also be applied to target coding sequences, as well as to target other important kinds of noncoding regulatory elements, including lncRNAs, miRNAs, and chromatin structural anchor points.
Barriers to epilepsy care in central Uganda, a qualitative interview and focus group study involving PLWE and their caregivers
Authors: Kaddumukasa Mark , Nalubwama Haddy , Kaddumukasa Martin N , Lhatoo Samden , Sewankambo Nelson , Katabira Elly , Sajatovic Martha , Blixen Carol
Published: June 2019

Background

Epilepsy is a common neurological disease with substantial impact on the subject and their caretakers. This exploratory study identified barriers to care for persons living with epilepsy (PLWE) to develop a culturally acceptable nurse led self-management intervention for PLWE previously developed in the United States.

Methods

The study involving 48 participants (31 PLWE and 17 caregivers) with in depth interviews and focus groups was conducted. We obtained insights into barriers to care in PLWE and their caregivers. Using a thematic analytic procedure emphasizing the dominant themes the qualitative responses were analyzed. Median age of PLWE was 24 years (IQR 19–30), and10 (52.6%) were male. The median age of epilepsy onset was 12 years (IQR 6 – 18), range of 1 -37 years. The median age of caregivers was 50 years (IQR 45 – 50.5), with a range of 18 -78 years. Seventy five percent of caregivers (6/8) were females.

Results

Three major areas of perceived barriers involving individual, family or community and provider and healthcare system barriers to epilepsy care emerged. Individual factors like limited epilepsy knowledge and medication non-adherence were reported to be key barriers to epilepsy care. Caregiver burden and lack of family support as well as poor health care access were identified from the family and health care systems.

Conclusions

The main barrier to epilepsy care is limited epilepsy knowledge in PLWE and their caregivers. Improving epilepsy care awareness and knowledge within communities and appropriate health care provider service for epilepsy would help reduce epilepsy barriers and improve care.
A Randomised Controlled Trial on Analgesic Effect of Repeated Quadratus Lumborum Block Versus Continuous Epidural Analgesia Following Laparoscopic Nephrectomy
Authors: Aditianingsih Dita , Pryambodho Pryambodho , Anasy Naufal , Tantri Aida Rosita , Mochtar Chaidir Arif
Published: June 2019

Background Epidural analgesia as the pain management for abdominal surgery has side effects such as paraesthesia, hypotension, haematomas, and impaired motoric of lower limbs. The quadratus lumborum block (QLB) has potential as an abdominal truncal block, however, the analgesic efficacy of QLB compared to epidural analgesia is unknown. This prospective randomised controlled study compared the effectiveness of QLB on postoperative opioid requirement and pain intensity with the epidural analgesia technique in transperitoneal laparoscopic nephrectomy. Methods Sixty-two patients underwent laparoscopic donor nephrectomy were randomised to receive QLB (n=31) or continuous epidural (n=31). The QLB group received bilateral QLB with 0.3–0.4 ml/kg bupivacaine 0.25% and the epidural group received bupivacaine 0.25% 6 ml/h for intraoperative analgesia. As postoperative analgesia, the QLB group received repeated bilateral QLB and the epidural group received the decreased dosage of bupivacaine 0.125% 6 ml/h for 24 hours after surgery completion. The primary outcome was cumulative morphine requirement 24 hours postoperatively. Secondary outcomes included haemodynamic changes, postoperative pain scores, sensory block coverage, Bromage score, postoperative nausea and vomiting (PONV), and duration of urinary catheterisation. Result Postoperative cumulative morphine requirement, pain scores, PONV and Bromage score were not significantly different between the QLB and epidural group. The QLB affected T9–L2, continuous epidural block affected T8–L3 dermatomes. Duration of urinary catheterisation was shorter (p < 0.001) in the QLB group. The mean arterial pressure (MAP) measured at 24 hours after surgery was lower in the epidural group (p = 0.001). Conclusion The repeated QLB had similar cumulative 24-h morphine requirement, higher MAP, similar postoperative pain scores, similar PONV and degree of motor and sensory blockade, and shorter urinary catheterisation duration, compared with continuous epidural analgesia after transperitoneal laparoscopic nephrectomy. Trial Registration ClinicalTrial.gov NCT03520205 retrospectively registered on May 9th 2018.
Exclusion process subject to conformational size changes
Authors: Rousset Yvan , Ciandrini Luca , Kern Norbert
Published: June 2019

We introduce a model for stochastic transport on a one-dimensional
substrate with particles assuming different conformations during their
stepping cycles. These conformations correspond to different footprints
on the substrate: in order to advance, particles are subject to
successive contraction and expansion steps with different characteristic
rates. We thus extend the paradigmatic exclusion process, provide
predictions for all regimes of these rates that are in excellent
agreement with simulations, and show that the current-density relation
may be affected considerably. Symmetries are discussed, and exploited.
We discuss our results in the context of molecular motors, confronting a
hand-over-hand and an inchworm stepping mechanism, as well as for
ribosomes.
Adaptación, validación y propiedades psicométricas de la “Escala de Evaluación del Estado de Crisis” (EEEC), en una muestra de estudiantes técnicos y universitarios en Colombia
Authors: Gonzalez-Cifuentes Carlos Eduardo , Cañas-Sánchez Ericka , Olaya-Higuera Martha , Nieto-Silva Carlos
Published: June 2019

El objetivo del presente estudio instrumental fue adaptar y analizar la validez y la fiabilidad de la “Escala de Evaluación del Estado de Crisis” (Crisis State Assessment Scale, CSAS; Lewis, 2005) en una muestra de 648 estudiantes colombianos en formación técnica o universitaria. La traducción y adaptación de la escala se realizó mediante la técnica de doble traducción, la versión en español resultante fue sometida a un estudio instrumental exploratorio con 129 participantes. Se obtuvo un alfa Cronbach total de 0,84 indicando una buena consistencia interna del instrumento adaptado, por lo que se procedió a la fase de validación factorial. El instrumento evalúa los siguientes tres constructos psicológicos: la intensidad del estado de crisis psicológica, la percepción del evento estresante y la percepción de los problemas de afrontamiento. El coeficiente alfa de Cronbach en la muestra de validación fue de .88 (n = 648), indicando una adecuada exactitud en la medida instrumental. El análisis factorial exploratorio de ejes principales con rotación promax arrojó una estructura bifactorial bien definida que explica el 60% de la varianza común. En el análisis factorial confirmatorio, los mejores índices de ajuste entre los datos empíricos y los modelos teóricos probados, corresponde al modelo que postula que existen dos factores de primer orden, Percepción del Evento Estresante “PEE” y Problemas Percibidos de Afrontamiento “PPA”, y que están explicados por un factor emergente de segundo orden que correspondería al constructo del Estado de Crisis “EEEC”. Los datos anteriores soportan una validez de constructo congruente con la teoría transaccional del estrés de Lazarus y Folkman (1984). Las correlaciones de Pearson entre el puntaje total y la “Escala de Estrés Percibido” fue de .70, apoyando la validez concurrente. En conclusión, estos resultados sugieren que la versión adaptada al español de la “Escala de Evaluación del Estado de Crisis” (EEEC) posee propiedades psicométricas satisfactorias para ser utilizada en la población colombiana y que los ítems que la componen son comprendidos sin problemas de adecuación cultural.
Velocity Control of Visco-Elastic Materials Movement in the Cylindrical Channel of the Grinder Feed Screw of the Former
Authors: Фоменко Екатерина , Fomenko Ekaterina , Нугманов Альберт , Nugmanov Albert
Published: June 2019

The present research features the problems of wheat processing. Wheat processing has its own specific features. For instance, the process of gluten extrusion forming is very complex since it is associated with the visco-elastic and adhesive properties of raw gluten. The article discusses the results of applying the numerical finite difference method to the Navier-Stokes equation in the case of the one-dimensional problem when a cooled viscoelastic material has to pass through circular nozzles. The paper also features the obtained surface model of velocity evolution and some averaged results for the possible automation of the process. The viscosity properties of raw gluten are variable and depend on temperature, chemical composition, and properties of the raw material. Modeling makes it possible to characterize the properties of the material and its behavior in various situations. Such research demands neither additional time nor significant costs. The authors identified patterns of movement for raw gluten in the extrusion molding unit and selected the most appropriate automation system to control the speed of its movement to the molding assembly in the grinder feed screw. The significance of the research is obvious for subsequent physical and mathematical modeling of heat and mass transfer processes of vacuum freezing and drying and granulating of gluten extrusions. The results of the research presented in the article are consistent with the available information on this topic. The present approach to solving the problem of choosing the best rational hydrodynamic regimes was applied due to the complexity of the experimental determination of velocity fields and the difficulty of analyzing the Navier-Stokes system of hydrodynamic differential equations with variable proportionality coefficients.
Kemerovo chocolate market: consumer preferences
Authors: Шемчук Мария , Shemchuk Mariya , Лобач Евгения , Lobach Evgeniya
Published: June 2019

Currently, chocolate and chocolate products remain quite popular among various consumer groups. According to experts, the average Russian consumes 4.7 kg of chocolate and chocolate products per year. The Russian chocolate market is 91% Russian-made. The market offers a wide range of products for various market segments. The high level of competition among the major domestic producers stimulates them to systematically study consumer preferences and adapt to them. In contrast with the central part of Russia, such market novelties as chocolate with salt, pepper, and other specific additives take root quite slowly in the non-metropolitan areas. The present research features the Kemerovo market of chocolate products and the preferences of Kemerovo residents. The main research method was questioning. The study revealed that the main target audience is the economically active female population aged 18–39. The greatest preference is given to milk chocolate in the middle price segment. As for the most preferred chocolate packaging, most respondents singled out plastic and paper packaging. As a rule, chocolate is purchased at a supermarket. Consumers are positive about new products but prefer to buy their favorite types of chocolate. The data obtained allowed the authors to formulate recommendations for chocolate producers taking into account the requirements of the main target audience, their preferences in taste, packaging, and the place of purchase.
New Biotechnology for the Production of Phytocompounds from Secondary Products of Grain Processing
Authors: Битюкова Анна , Bityukova Anna , Амелькина Александра , Amelkina Alexandra , Евтеев Александр , Evteev Alexander , Банникова Анна , Bannikova Anna
Published: June 2019

The present research features a new biotechnological approach for complex processing of secondary cereal products. The approach makes it possible to obtain new functional foods and ingredients, such as carbohydrate-protein concentrate, dietary fiber concentrates, biologically active substances, polyphenols, and xylo-oligosaccharides. The complex biotechnology involves oat bran biotransformation and includes chemical, hydrothermal, and enzymatic methods of extraction, which allows manufacturers to obtain functional ingredients with antioxidant and prebiotic properties. The concentrates obtained as a result of the study contained a significant amount of protein, hydrolysis products of polysaccharides (glucose, maltodextrins), and free polyphenols. The experiment produced concentrates of biologically active substances containing xylo-oligosaccharides and phenolic compounds. The content of polyphenols was 67% of the total amount in oat bran, protein – 6.9%, carbohydrates – 80.7% xylo-oligosaccharides whith prebiotic properties 35.3%–71.5%, and ash 11.3%. The obtained data indicated a high antioxidant activity of polyphenol extracts. The differences in antioxidant activity between various methods of extraction are associated primarily with the completeness of extraction and the stability of the extracted phenolic compounds. Ultrasonic treatment proved beneficial for the extraction kinetics and the polyphenol yield at the initial stage, with an increase in antioxidant properties. As for the antioxidant activity of polyphenol concentrate during storage, the research did not reveal changes for 8 months at a temperature of 20 ± 1°C and a relative humidity of 70 ± 5%. The study of the growth dynamics of Lactobacillus acidophilus and Bifi obacterium bifi um in media containing xylo-oligosaccharide concentrate confirmed the presence of prebiotic properties and their selectivity. The accumulation of biomass of prebiotic cultures occurred faster with the use of nutrient media with the use of xylo-oligosaccharide concentrate and lactulose, as compared with milk. Thus, the research confirmed the feasibility of biotechnology for transforming oat bran into functional ingredients, which makes it possible to use them in new technological solutions for products with bifidogenic properties.
Electrocoagulation of azo dye containing synthetic wastewater using monopolar iron electrodes and the characterization of the sludge
Authors: Suresh A. , Sathish S. , Narendrakumar G.
Published: June 2019

In this study, the decolourisation of Congo red dye is studied using the natural coagulant Maize Seed Powder (MSP); a suitable alternative for chemical coagulants in electro coagulation (EC) using iron electrodes. The effects of various parameters such as initial dye concentration, pH, applied voltage and time on the decolourisation of dye were studied. The cost of water treatment is increasing due to suspended and colloidal particle load. The coagulation process can be used as a primary step for water and wastewater treatment processes. Among plant materials that have been tested, the seeds of maize have been shown to be one of the most effective primary coagulants in water and wastewater treatment. The removal efficiency of the dye with and without the presence of MSP as a coagulant aid was determined. In the EC process maximum Congo red removal was achieved by using the natural coagulant MSP. Congo red (CR) decolourisation efficiency is 95.23% at a coagulant dosage of 50 mg/lit, pH of 5, operating time of 15 minutes, and a power of 40 volts with iron electrodes. In the absence of coagulant MSP, Congo red decolourisation efficiency was found to be 80.31% at pH of 5, operating time of 15 minutes, and a power of 40 volts with iron electrodes.
Protein Post-Translational Modification Crosstalk in Acute Myeloid Leukemia Calls for Action
Authors: Hernandez-Valladares Maria , Wangen Rebecca , Berven Frode S. , Guldbrandsen Astrid
Published: June 2019

Background:

Post-translational modification (PTM) crosstalk is a young research field.
However, there is now evidence of the extraordinary characterization of the different proteoforms
and their interactions in a biological environment that PTM crosstalk studies can describe. Besides
gene expression and phosphorylation profiling of acute myeloid leukemia (AML) samples, the
functional combination of several PTMs that might contribute to a better understanding of the
complexity of the AML proteome remains to be discovered.

Objective:

By reviewing current workflows for the simultaneous enrichment of several PTMs and
bioinformatics tools to analyze mass spectrometry (MS)-based data, our major objective is to introduce
the PTM crosstalk field to the AML research community.

Results:

After an introduction to PTMs and PTM crosstalk, this review introduces several protocols
for the simultaneous enrichment of PTMs. Two of them allow a simultaneous enrichment of at
least three PTMs when using 0.5-2 mg of cell lysate. We have reviewed many of the bioinformatics
tools used for PTM crosstalk discovery as its complex data analysis, mainly generated from
MS, becomes challenging for most AML researchers. We have presented several non-AML PTM
crosstalk studies throughout the review in order to show how important the characterization of
PTM crosstalk becomes for the selection of disease biomarkers and therapeutic targets.

Conclusion:

Herein, we have reviewed the advances and pitfalls of the emerging PTM crosstalk
field and its potential contribution to unravel the heterogeneity of AML. The complexity of sample
preparation and bioinformatics workflows demands a good interaction between experts of several
areas.
iMTBGO: An Algorithm for Integrating Metabolic Networks with Transcriptomes Based on Gene Ontology Analysis
Authors: Mao Zhitao , Ma Hongwu
Published: June 2019

Background:

Constraint-based metabolic network models have been widely used in phenotypic prediction and metabolic engineering design. In recent years, researchers have attempted to improve prediction accuracy by integrating regulatory information and multiple types of “omics” data into this constraint-based model. The transcriptome is the most commonly used data type in integration, and a large number of FBA (flux balance analysis)-based integrated algorithms have been developed.

Methods and Results:

We mapped the Kcat values to the tree structure of GO terms and found that the Kcat values under the same GO term have a higher similarity. Based on this observation, we developed a new method, called iMTBGO, to predict metabolic flux distributions by constraining reaction boundaries based on gene expression ratios normalized by marker genes under the same GO term. We applied this method to previously published data and compared the prediction results with other metabolic flux analysis methods which also utilize gene expression data. The prediction errors of iMTBGO for both growth rates and fluxes in the central metabolic pathways were smaller than those of earlier published methods.

Conclusion:

Considering the fact that reaction rates are not only determined by genes/expression levels, but also by the specific activities of enzymes, the iMTBGO method allows us to make more precise predictions of metabolic fluxes by using expression values normalized based on GO.
Monocytes as Carriers of Magnetic Nanoparticles for Tracking Inflammation in the Epileptic Rat Brain
Authors: Han Hadas , Eyal Sara , Portnoy Emma , Mann Aniv , Shmuel Miriam , Benifla Mony , Ekstein Dana , Polyak Boris
Published: June 2019

Background:

Inflammation is a hallmark of epileptogenic brain tissue. Previously, we have
shown that inflammation in epilepsy can be delineated using systemically-injected fluorescent and magnetite-
laden nanoparticles. Suggested mechanisms included distribution of free nanoparticles across a
compromised blood-brain barrier or their transfer by monocytes that infiltrate the epileptic brain.

Objective:

In the current study, we evaluated monocytes as vehicles that deliver nanoparticles into the
epileptic brain. We also assessed the effect of epilepsy on the systemic distribution of nanoparticleloaded
monocytes.

Methods:

The in vitro uptake of 300-nm nanoparticles labeled with magnetite and BODIPY (for optical
imaging) was evaluated using rat monocytes and fluorescence detection. For in vivo studies we used the
rat lithium-pilocarpine model of temporal lobe epilepsy. In vivo nanoparticle distribution was evaluated
using immunohistochemistry.

Results:

89% of nanoparticle loading into rat monocytes was accomplished within 8 hours, enabling
overnight nanoparticle loading ex vivo. The dose-normalized distribution of nanoparticle-loaded monocytes
into the hippocampal CA1 and dentate gyrus of rats with spontaneous seizures was 176-fold and
380-fold higher compared to the free nanoparticles (p<0.05). Seizures were associated with greater
nanoparticle accumulation within the liver and the spleen (p<0.05).

Conclusion:

Nanoparticle-loaded monocytes are attracted to epileptogenic brain tissue and may be used
for labeling or targeting it, while significantly reducing the systemic dose of potentially toxic compounds.
The effect of seizures on monocyte biodistribution should be further explored to better
understand the systemic effects of epilepsy.
Inhibitors of Cyclin-Dependent Kinase 1/2 for Anticancer Treatment
Authors: Mou Jiajia , Chen Danghui , Deng Yanru
Published: June 2019

Background:

The cell cycle is regulated by cyclin-dependent kinases (CDKs) and their
cognate cyclins, along with their endogenous inhibitors (CDKIs). CDKs act as central regulators in
this process. Different CDKs play relevant roles in different phases. Among all CDKs, CDK1 is
indispensible, which can drive all events that are required in the cell cycle in the absence of interphase
CDKs (CDK2, CDK3, CDK4 and CDK6). So, CDK1 is an attractive target for anticancer
drug development.

Methods:

CDK1 and CDK2 have 89.19% similar residues and 74.32% identical residues, their
structures especially the ATP-binding sites are of great similarity. So, it is difficult to inhibit
CDK1 and CDK2 individually. In this review, recent advances about CDK1/2 inhibitors were
summarized. The chemical structures of different classes of CDK1/2 inhibitors and their structure
activity are presented.

Results:

19 kinds of CDK1/2 or CDK1 inhibitors with different scaffolds, including CDK2 allosteric
inhibitors, were summarized. Some inhibitors are nature derived, for example, phenanthrene
derivatives, nortopsentin derivatives, variolin B derivatives and meridians.

Conclusion:

Nature products, especially marine ones are potential resources for CDK1 inhibitors
development. The findings of CDK2 allosteric inhibitors open an avenue to the discovery of novel
selective CDK1 or other CDKs allosteric inhibitors.
Design, Synthesis, and Evaluation of Isoquinoline Ureas as TRPV1 Antagonists
Authors: Gujarati Nehaben A. , Undem Bradley J. , Korlipara Vijaya L.
Published: June 2019

Background:

The inhibition of transient receptor potential vanilloid receptor 1
(TRPV1) has emerged as a novel approach for the treatment of various pain states. Pyrrolidinyl
urea, SB 705498 with pKb = 7.3 in guinea pig TRPV1 receptor has been investigated in Phase II
clinical trials for pain and chronic cough. Another heteroaryl urea derivative, A-425619 1, has
been reported to be a potent and selective TRPV1 antagonist of capsaicin-evoked receptor activation
with an IC50 value of 4 nM in hTRPV1.

Objective:

A series of thirteen A-425619 1 analogues with modifications centered around the Cregion
were synthesized to understand the binding site characteristics of TRPV1 receptors.

Method:

We synthesized a series of isoquinoline ureas and evaluated their antagonist potency using
smooth muscle assay using guinea pig trachea along with the evaluation of the molecular properties
and molecular modeling using CoMFA studies.

Results:

p-Chloro 4, p-bromo 5, m-isothiocyanate 15, and p-isothiocyanate 16 derivatives were
found to be the most potent members of the series with pKb values in the range of 7.3-7.4 in the
functional assay using guinea pig trachea. The lead compound A-425619 1 exhibited a pKb value
of 8.1 in this assay.

Conclusion:

The para-substituted analogues were found to be more potent than the ortho- and
meta- analogues in the biological assay. This observation was further supported by molecular
modeling studies using CoMFA.
Overcoming Cancer Cell Drug Resistance by a Folic Acid Targeted Polymeric Conjugate of Buthionine Sulfoximine
Authors: Cilurzo Felisa , Cristiano Maria C. , Da Pian Marta , Cianflone Eleonora , Quintieri Luigi , Paolino Donatella , Pasut Gianfranco
Published: June 2019

Background:

Glutathione (GSH), which is the predominant low molecular weight intracellular thiol in mammals, has multiple functions, such as those of protecting against oxidative stress and detoxifying endogenous and exogenous electrophiles. High GSH levels, which have been observed in various types of tumors, have been thought to contribute to the resistance of neoplastic cells to apoptotic stimuli triggered by pro-oxidant therapy. Although L-(S,R)-Buthionine Sulfoximine (BSO), a selective irreversible inhibitor of glutamate cysteine ligase, depletes GSH in vitro and in in vivo and sensitizes tumor cells to radiation and some cancer chemotherapeutics, its toxicity and short in vivo half-life have limited its application to combination anticancer therapies.

Objective:

To demonstrate that a folate-targeted PEGylated BSO conjugate can sensitize cancer cells to a Reactive Oxygen Species (ROS)-generating anticancer agent by depleting GSH.

Methods:

A novel folate-targeted PEGylated-BSO conjugate was synthesized and tested in combination with gemcitabine in human cell lines that over-express (HeLa) or do not express (A549) the folate receptor.

Results:

The prepared folate-PEG-GFLG-BSO conjugate proved to be efficacious in reducing GSH levels and, when used in combination with the pro-oxidant drug gemcitabine, it enhanced drug activity in the cell line overexpressing the folate receptor.

Conclusion:

The folate-PEG-GFLG-BSO conjugate studied was found to be effective in sensitizing folatereceptor positive cancer cells to the ROS-generating drug gemcitabine.