Traditional and confocal descriptions of a new genus and two new species of deep water Cerviniinae Sars, 1903 from the Southern Atlantic and the Norwegian Sea: with a discussion on the use of digital media in taxonomy (Copepoda, Harpacticoida, Aegisthidae)
Authors: Corgosinho Paulo H. C. , Kihara Terue C. , Schizas Nikolaos V. , Ostmann Alexandra , Arbizu Pedro Martínez , Ivanenko Viatcheslav N. 
Published: June 2018

Aegisthidae is one of the most abundant and diverse families of harpacticoid copepods living in deep-sea benthos, and the phylogenetic relationships within the family are in state of flux. Females of two new deep-water species of harpacticoid copepods belonging to the Hasegen. n. (Aegisthidae: Cerviniinae) are described. The first taxonomic description of marine copepod species based on the combined use of interference and confocal microscopy for the study of the habitus and dissected appendages is presented here. CLSM (Confocal Laser Scanning Microscopy) is a non-destructive method, comparable in quality to SEM (scanning electron microscopy) at the same magnifications. To observe and reconstruct in detail the habitus and dissected appendages, whole specimens and dissected parts were stained with Congo Red, mounted on slides with glycerine for CLSM and scanned under three visible-light lasers. Hase lagomorphicusgen. et sp. n. and Hase talpamorphicusgen. et sp. n. were collected from the sediments of the Southern Atlantic and the Norwegian Sea, from 2270 m and 5468 m depths, respectively. Hasegen. n. is included within Cerviniinae based on the caudal rami which are relatively divergent. Hasegen. n. is the sister taxon of Cerviniella based on the following synapomorphies: sturdy body, exopodites 1–3 of pereopods 1–3 heavily built, transformed into digging limbs, with strong outer and distal spines/setae, two-segmented endopod on the pereopods 2 and 3, and a reduced pereopod 5. Compared to Cerviniella, Hasegen. n. exhibits a more developed armature on the pereopod 1, which has outer and distal elements transformed into strong and long spines vs. stiff setae on Cerviniella.Hasegen. n. has one or two strong and long spines on the inner margin of the exopodite 3 of pereopod 4 and pereopod 5 is fused to the somite, ornamented with three distal setae. The telson of Hasegen. n. is subquadratic, and the furca is among the shortest yet described for Aegisthidae. The new species differ in a number of diagnostic characters, three of which are: a) the somite bearing pereopods 3 and 4 with latero-distal spiniform processes in H. talpamorphicusgen. et sp. n. but smooth in H. lagomorphicusgen. et sp. n., b) antenna is armed with three stout spines on the lateral inner margin of the exopod in H. talpamorphicusgen. et sp. n. and two proximal setae in H. lagomorphicusgen. et sp. n., and c) pereopod 4 exopodite 3 has two long and strong spines on the inner margin in H. lagomorphicusgen. et sp. n. and one spine in H. talpamorphicusgen. et sp. n. The high quality of CLSM images should foster discussion about the use of high quality digital images as type or as part of the type series in zoological studies, especially when studying rare and small macrofaunal and meiofaunal taxa.