A shared group of bacterial taxa in the duodenal microbiota of undernourished Pakistani children with environmental enteric dysfunction
Authors: Iqbal Najeeha T. , Chen Robert Y. , Griffin Nicholas W. , Hibberd Matthew C. , Khalid Aqsa , Sadiq Kamran , Jamil Zehra , Ahmed Kumail , Iqbal Junaid , Hotwani Aneeta , Kabir Furqan , Rahman Najeeb , Rizvi Arjumand , Idress Romana , Ahmed Zubair , Ahmed Sheraz , Umrani Fayaz , Syed Sana , Moore Sean R. , Ali Asad , Barratt Michael J. , Gordon Jeffrey I. 
Published: May 2024

Environmental enteric dysfunction (EED) is a subclinical syndrome of altered small intestinal function postulated to be an important contributor to childhood undernutrition. The role of small intestinal bacterial communities in the pathophysiology of EED is poorly defined due to a paucity of studies where there has been a direct collection of small intestinal samples from undernourished children. Sixty-three members of a Pakistani cohort identified as being acutely malnourished between 3 and 6 months of age and whose wasting (weight-for-length
Z
-score [WLZ]) failed to improve after a 2-month nutritional intervention underwent esophagogastroduodenoscopy (EGD). Paired duodenal luminal aspirates and duodenal mucosal biopsies were obtained from 43 children. Duodenal microbiota composition was characterized by sequencing bacterial 16S rRNA gene amplicons. Levels of bacterial taxa (amplicon sequence variants [ASVs]) were referenced to anthropometric indices, histopathologic severity in biopsies, expression of selected genes in the duodenal mucosa, and fecal levels of an immunoinflammatory biomarker (lipocalin-2). A “core” group of eight bacterial ASVs was present in the duodenal samples of 69% of participants.
Streptococcus anginosus
was the most prevalent, followed by
Streptococcus
sp.,
Gemella haemolysans
,
Streptococcus australis
,
Granulicatella elegans
,
Granulicatella adiacens,
and
Abiotrophia defectiva
. At the time of EGD, none of the core taxa were significantly correlated with WLZ. Statistically significant correlations were documented between the abundances of
Granulicatella elegans
and
Granulicatella adiacens
and the expression of duodenal mucosal genes involved in immune responses (dual oxidase maturation factor 2, serum amyloid A, and granzyme H). These results suggest that a potential role for members of the oral microbiota in pathogenesis, notably
Streptococcus
,
Gemella,
and
Granulicatella
species, warrants further investigation.

IMPORTANCE

Undernutrition among women and children is a pressing global health problem. Environmental enteric dysfunction (EED) is a disease of the small intestine (SI) associated with impaired gut mucosal barrier function and reduced capacity for nutrient absorption. The cause of EED is ill-defined. One emerging hypothesis is that alterations in the SI microbiota contribute to EED. We performed a culture-independent analysis of the SI microbiota of a cohort of Pakistani children with undernutrition who had failed a standard nutritional intervention, underwent upper gastrointestinal tract endoscopy, and had histologic evidence of EED in their duodenal mucosal biopsies. The results revealed a shared group of bacterial taxa in their duodenums whose absolute abundances were correlated with levels of the expression of genes in the duodenal mucosa that are involved in inflammatory responses. A number of these bacterial taxa are more typically found in the oral microbiota, a finding that has potential physiologic and therapeutic implications.