bluuebunny/crossref_metadata_2025 huggingface dataset:
Identifying Actionable Variants Using Capture-Based Targeted Sequencing in 563 Patients With Non-Small Cell Lung Carcinoma
Authors: Jiang Haiping , Wang Yinan , Xu Hanlin , Lei Wei , Yu Xiaoyun , Tian Haiying , Meng Cong , Wang Xueying , Zhao Zicheng , Jin Xiangfeng
Published: February 2022

Although the NSCLC diagnostic standards recommend the detection of driver gene mutation, comprehensive genomic profiling has not been used widely in clinical practice. As to the different mutation spectrum characteristics between populations, the research based on Chinese NSCLC cohort is very important for clinical practice. Therefore, we collected 563 surgical specimens from patients with non-small cell lung carcinoma and applied capture-based sequencing using eight-gene panel. We identified 556 variants, with 416 potentially actionable variants in 54.88% (309/563) patients. These single nucleotide variants, insertions and deletions were most commonly found in EGFR (55%), followed by ERBB2 (12%), KRAS (11%), PIK3CA (9%), MET (8%), BRAF (7%), DDR2 (2%), NRAS (0.3%). By using ten protein function prediction algorithms, we also identified 30 novel potentially pathogenic variants. Ninety-eight patients harbored EFGR exon 21 p.L858R mutation and the catalytic domain of the protein tyrosine kinase (PTKc) in EGFR is largely mutated. In addition, there were nine frequent pathogenic variants found in five or more patients. This data provides the potential molecular basis for directing the treatment of lung cancer.
Analgesic Effects of Oxycodone in Combination With Risperidone or Ziprasidone: Results From a Pilot Randomized Controlled Trial in Healthy Volunteers
Authors: Nagpal Ameet S. , Lodge Daniel J. , Potter Jennifer S. , Frazer Alan , Tragus Robin , Curtis Megan E. , Boley Angela M. , Eckmann Maxim
Published: February 2022

Background and Objectives

Patients taking opioids are at risk of developing dependence and possibly abuse. Given the role of the mesolimbic dopamine system in opioid reward, blocking dopamine D2 receptors should limit the abuse liability of opioid analgesics. This pilot study evaluates the analgesic efficacy of oxycodone combined with an atypical antipsychotic (dopamine D2 receptor antagonist).

Methods

A randomized, double-blind, within-subjects, controlled trial in healthy volunteers was conducted at UT Health SA Pain Clinic. Fifteen volunteers with previous medical exposure to opioids were enrolled. Risperidone (2 mg) or ziprasidone (80 mg) in combination with oxycodone (5, 10, 15 mg) was administered. Pain intensity using the cold pressor test, Current Opioid Misuse Measure (COMM), Addiction Research Center Inventory (ARCI, opioid subscale), Drug likability with drug effects questionnaire (DEQ) were assessed.

Results

Oxycodone produced dose dependent increases in thermal analgesia on the cold pressor test that was significant at 10 and 15 mg (t = 3.087, P = 0.017). The combination did not significantly alter thermal analgesia. There was no significant effect of the combination on the ARCI or the POMS.

Discussion and Conclusion

The combination of an atypical antipsychotic with oxycodone does not alter analgesic response or increase the incidence of adverse effects when compared to oxycodone alone. Such information is critical for the development of drug combinations for the treatment of pain and provide the foundation for future studies of abuse potential in drug users.

Scientific Significance

This intervention in chronic pain patients is unique because it utilizes FDA approved drugs in combination to reduce abuse liability. The first step, and aim of this study, is to confirm the drug combination does not interfere with analgesic efficacy. The next step is to examine the combination in recreational drug users to assess the potential to block the euphoric effects of oxycodone. Ultimately, if this combination is effective, this approach could be beneficial in management of chronic pain.
Translational Strategies to Eliminate Chronic Hepatitis B in Children: Prophylaxis and Management in East Asian Countries
Authors: Kang Ben , Yi Dae Yong , Choe Byung-Ho
Published: February 2022

Translational medical research on hepatitis B virus (HBV) infection and chronic hepatitis B (CHB) pathogenesis provides guidance on strengthening the treatment and prevention strategies of CHB. Preventing vertical transmission is the key to eliminating HBV infection in children. The understanding of HBV replication, hepatocyte turnover, and the fate of covalently closed circular DNA (cccDNA) would help establish a personalized application of the guidelines, especially concerning the discontinuation of nucleos(t)ide analog (NA) treatment in children. Transplacental leakage of HBV-infected maternal blood is suggested as the leading cause of vertical transmission. Prenatal maternal prophylaxis could diminish maternal HBV viremia at delivery, to reduce the risk of neonatal HBV infection. The meaning of the expression “no additional risk of breast milk feeding” is thereby explained. Understanding the untreated natural course of CHB in children and the course changeable by treatment is important to apply individualistic strategies and avoid the immoral selection of treatment indications. NAs with potent efficacy and a high barrier to drug resistance should be used as first-line treatment to reduce the likelihood of NA-resistant HBV development because the rate of mutant HBV emergence might count on the infected hepatocyte turnover rate in chronic HBV infection. Although elimination of intranuclear cccDNA is difficult by NAs alone, a cure is possible by human immunity and hepatocyte turnover. The reduction of intranuclear cccDNA occurs after the destruction of HBV-infected hepatocytes, non-cytolytic immune response, apoptosis of hepatocytes, and compensatory cell proliferation. Therefore, consolidation therapy after NA-induced hepatitis B e-antigen seroconversion must be necessary for a sufficient period. This review also summarizes the treatment strategies of CHB in children based on the practical application of translational research.
Analysis of Influence Factors of Negative Skin Friction on PHC Pile in High-Backfill Sites
Authors: Gen Xiong , Ben He , Bing Pan , Xiangming Ge , Weijiang Chu
Published: February 2022

It is recognized that NSF on a pile affects the structural and geotechnical design. The formation of NSF on a pre-stressed high-strength concrete (PHC) pile under a control building in a Vietnamese project was investigated using a verified numerical model in FLAC3D. The height of backfills, the consolidation degree of soil, working loads on the PHC pile, and the pile diameter were analyzed in sensitivity analyses to investigate the influence on NSF. It was found that higher backfills and higher consolidation degree were dominant in the formation of NSF, causing an increase of the dragload and an upward movement of the neutral plane. The working load and the pile diameter affected the dragload and the position of the neutral plane less. A limit decrease of the dragload induced by NSF was observed when the working load increased. Accelerating the soil consolidation process would be suggested to avoid great displacement due to NSF.
Second Harmonic Generation Properties in Chiral Sarcomeres of Drosophila Larval Muscles
Authors: Karunendiran Abiramy , Mirsanaye Kamdin , Stewart Bryan A. , Barzda Virginijus
Published: February 2022

Polarimetric second harmonic generation (SHG) microscopy is a label-free ultrastructural imaging technique that is applied to study molecular organization in striated muscle structure. Wild type OreR and UNC45 knockdown Drosophila melanogaster somatic larva body wall muscles are used to elucidate the influence of myosin organization on SHG imaging response. Structural changes due to varying myosin content are studied with linear polarization-in polarization-out (PIPO) SHG microscopy. PIPO SHG microscopy revealed changes in the striated pattern of somatic muscles and a reduced signal intensity correlated with reduced order of myosin filaments. The achiral and chiral susceptibility component ratios R- and C-ratios, respectively, were deduced. R-ratio values were higher in the UNC45 knockdown larva mutant compared to control, suggesting that in addition to myosin, another protein structure provide basis for a second harmonic response. The SHG active protein structure is located in the region of isotropic bands of the striated muscles. The measured C-ratio from the muscle fibers appeared to be small. Nonetheless, the wide distribution of C-ratio values of myosin filaments could be observed in the wild-type OreR muscles. Overall, this technique provides insight on how second harmonic properties change with myosin filament structure.
Transcriptomic Regulation of Muscle Mitochondria and Calcium Signaling by Insulin/IGF-1 Receptors Depends on FoxO Transcription Factors
Authors: Bhardwaj Gourav , Penniman Christie M. , Klaus Katherine , Weatherford Eric T. , Pan Hui , Dreyfuss Jonathan M. , Nair K. Sreekumaran , Kahn C. Ronald , O’Neill Brian T.
Published: February 2022

Insulin and IGF-1, acting through the insulin receptor (IR) and IGF-1 receptor (IGF1R), maintain muscle mass and mitochondrial function, at least part of which occurs via their action to regulate gene expression. Here, we show that while muscle-specific deletion of IR or IGF1R individually results in only modest changes in the muscle transcriptome, combined deletion of IR/IGF1R (MIGIRKO) altered > 3000 genes, including genes involved in mitochondrial dysfunction, fibrosis, cardiac hypertrophy, and pathways related to estrogen receptor, protein kinase A (PKA), and calcium signaling. Functionally, this was associated with decreased mitochondrial respiration and increased ROS production in MIGIRKO muscle. To determine the role of FoxOs in these changes, we performed RNA-Seq on mice with muscle-specific deletion of FoxO1/3/4 (M-FoxO TKO) or combined deletion of IR, IGF1R, and FoxO1/3/4 in a muscle quintuple knockout (M-QKO). This revealed that among IR/IGF1R regulated genes, >97% were FoxO-dependent, and their expression was normalized in M-FoxO TKO and M-QKO muscle. FoxO-dependent genes were related to oxidative phosphorylation, inflammatory signaling, and TCA cycle. Metabolomic analysis showed accumulation of TCA cycle metabolites in MIGIRKO, which was reversed in M-QKO muscle. Likewise, calcium signaling genes involved in PKA signaling and sarcoplasmic reticulum calcium homeostasis were markedly altered in MIGIRKO muscle but normalized in M-QKO. Thus, combined loss of insulin and IGF-1 action in muscle transcriptionally alters mitochondrial function and multiple regulatory and signaling pathways, and these changes are mediated by FoxO transcription factors.
A Systematic Review of Thermosensation and Thermoregulation in Anxiety Disorders
Authors: Fischer Susanne , Haas Florence , Strahler Jana
Published: February 2022

Graphical Abstract

This systematic review sought to summarise studies comparing thermosensory and thermoregulatory functioning between individuals with anxiety disorders and healthy controls. Its main findings are summarised on the right side.
Protective Effect of Moderate Hypotonic Fluid on Organ Dysfunction via Alleviating Lethal Triad Following Seawater Immersion With Hemorrhagic Shock in Rats
Authors: Zhu Yu , Deng Haoyue , She Han , Zhou Yuanqun , Wu Yue , Zhang Jie , Liu Liangming , Tao Li
Published: February 2022

Previous studies found that seawater immersion combined with hemorrhagic shock (SIHS) induced serious organ function disorder, and lethal triad was a critical sign. There were no effective treatments of SIHS. Fluid resuscitation was the initial measurement for early aid following hemorrhagic shock, while the proper fluid for SIHS is not clear. Effects of different osmotic pressures [lactated Ringer’s (LR) solution, 0.3% saline, 0.6% saline, and 0.9% normal saline] on the lethal triad, mitochondrial function, vital organ functions, and survival were observed following SIHS in rats. The results showed that SIHS led to an obvious lethal triad, which presented the decrease of the body temperature, acidosis, and coagulation functions disorder in rats. Fluid resuscitation with different osmotic pressures recovered the body temperature and corrected acidosis with different levels; effects of 0.6% normal saline were the best; especially for the coagulation function, 0.6% normal saline alleviated the lethal triad significantly. Further studies showed that SIHS resulted in the damage of the mitochondrial function of vital organs, the increase of the vascular permeability, and, at the same time, the organ function including cardiac, liver, and kidney was disordered. Conventional fluid such as LR or 0.9% normal saline could not improve the mitochondrial function and vascular leakage and alleviate the damage of the organ function. While moderate hypotonic fluid, the 0.6% normal saline, could lighten organ function damage via protecting mitochondrial function. The 0.6% normal saline increased the left ventricular fractional shortening and the left ventricular ejection fraction, and decreased the levels of aspartate transaminase, alanine transferase, blood urea nitrogen, and creatinine in the blood. The effects of fluids with different osmotic pressures on the mean arterial pressure (MAP) had a similar trend as above parameters. The survival results showed that the 0.6% normal saline group improved the survival rate and prolonged the survival time, the 72 h survival rate was 7/16, as compared with the LR group (3/16). The results indicate that appropriate hypotonic fluid is suitable after SIHS, which alleviates the lethal triad, protects the mitochondrial function and organ functions, and prolongs the survival time.
Soil Seed Bank Persistence Across Time and Burial Depth in Calcareous Grassland Habitats
Authors: Mašková Tereza , Poschlod Peter
Published: February 2022

Seed persistence in the soil is crucial for population dynamics. Interspecific differences in soil seed mortality could be a mechanism that may stimulate species coexistence in herbaceous plant communities. Therefore, understanding the levels and causes of seed persistence is vital for understanding community composition and population dynamics. In this study, we evaluated the burial depth as a significant predictor of the temporal dynamics of soil seed persistence. We suppose that species differ in this temporal dynamics of soil seed persistence according to burial depth. Furthermore, we expected that burial depth would affect soil seed persistence differently concerning the species-specific type of dormancy, light, and fluctuating temperature requirements for germination. Seeds of 28 herbaceous species of calcareous grasslands were buried in the field into depths of 1, 5, and 10 cm under the soil surface. Seed viability was tested by germination and tetrazolium tests several times for three years. Species-specific seed traits—a type of dormancy, light requirements and alternating temperature requirements for germination, and longevity index—were used for disentangling the links behind species-specific differences in soil seed persistence. Our study showed differences in soil seed persistence according to the burial depth at the interspecific level. Generally, the deeper the buried seeds, the longer they stayed viable, but huge differences were found between individual species. Species-specific seed traits seem to be an essential determinant of seed persistence in the soil. Seeds of dormant species survived less and only dormant seeds stayed viable in the soil. Similarly, seeds of species without light or alternating temperature requirements for germination generally remained viable in the soil in smaller numbers. Moreover, seeds of species that require light for germination stayed viable longer in the deeper soil layers. Our results help understand the ecosystem dynamics caused by seed reproduction and highlight the importance of a detailed long-term investigation of soil seed persistence. That is essential for understanding the fundamental ecological processes and could help restore valuable calcareous grassland habitats.
Genotypic Variation of Nitrogen Use Efficiency and Amino Acid Metabolism in Barley
Authors: Decouard Bérengère , Bailly Marlène , Rigault Martine , Marmagne Anne , Arkoun Mustapha , Soulay Fabienne , Caïus José , Paysant-Le Roux Christine , Louahlia Said , Jacquard Cédric , Esmaeel Qassim , Chardon Fabien , Masclaux-Daubresse Céline , Dellagi Alia
Published: February 2022

Owing to the large genetic diversity of barley and its resilience under harsh environments, this crop is of great value for agroecological transition and the need for reduction of nitrogen (N) fertilizers inputs. In the present work, we investigated the diversity of a North African barley genotype collection in terms of growth under limiting N (LN) or ample N (HN) supply and in terms of physiological traits including amino acid content in young seedlings. We identified a Moroccan variety, Laanaceur, accumulating five times more lysine in its leaves than the others under both N nutritional regimes. Physiological characterization of the barley collection showed the genetic diversity of barley adaptation strategies to LN and highlighted a genotype x environment interaction. In all genotypes, N limitation resulted in global biomass reduction, an increase in C concentration, and a higher resource allocation to the roots, indicating that this organ undergoes important adaptive metabolic activity. The most important diversity concerned leaf nitrogen use efficiency (LNUE), root nitrogen use efficiency (RNUE), root nitrogen uptake efficiency (RNUpE), and leaf nitrogen uptake efficiency (LNUpE). Using LNUE as a target trait reflecting barley capacity to deal with N limitation, this trait was positively correlated with plant nitrogen uptake efficiency (PNUpE) and RNUpE. Based on the LNUE trait, we determined three classes showing high, moderate, or low tolerance to N limitation. The transcriptomic approach showed that signaling, ionic transport, immunity, and stress response were the major functions affected by N supply. A candidate gene encoding the HvNRT2.10 transporter was commonly up-regulated under LN in the three barley genotypes investigated. Genes encoding key enzymes required for lysine biosynthesis in plants, dihydrodipicolinate synthase (DHPS) and the catabolic enzyme, the bifunctional Lys-ketoglutarate reductase/saccharopine dehydrogenase are up-regulated in Laanaceur and likely account for a hyperaccumulation of lysine in this genotype. Our work provides key physiological markers of North African barley response to low N availability in the early developmental stages.
Parents’ Education Anxiety and Children’s Academic Burnout: The Role of Parental Burnout and Family Function
Authors: Wu Kai , Wang Feng , Wang Wei , Li Yongxin
Published: February 2022

This study aimed to explore the effect of parents’ education anxiety on children’s academic burnout, and the mediation effect of parental burnout and the moderating effect of family function. A total of 259 paired parents and children from two middle schools in central China participated in the survey. The questionnaire was conducted using the Educational Anxiety Scale, Parental Burnout Scale, Adolescent Student Burnout Inventory, and Family APGAR Index. Our results indicated that parental education anxiety had a positive predictive effect on children’s academic burnout. Moreover, parental burnout played a complete mediating role between parents’ education anxiety and children’s academic burnout. Finally, the relationship between education anxiety and parental burnout was moderated by family function, and higher family function buffered the effect of education anxiety on parental burnout. The results suggest the mechanism of parental education anxiety on children’s academic burnout, and the role of family function in alleviating parental burnout.
Citizen Environmental Behavior From the Perspective of Psychological Distance Based on a Visual Analysis of Bibliometrics and Scientific Knowledge Mapping
Authors: Yang Li , Fang Xin , Zhu Junqi
Published: February 2022

Global warming and other climate issues seriously threaten global sustainable development. As citizen environmental behavior can have a positive impact on the environment, it is of great theoretical significance and practical reference value to study the impact of psychological distance theory on citizen environmental behavior. This study obtained 2,633 related studies from 1980 to 2020 from the Web of Science as research objects, and used CiteSpace, VOSviewer, Netdraw, and other software programs to perform a bibliometric analysis, which can show the complex relationship implied in the citation, intuitively grasp the development context and hot frontier in this field, and help scholars better study citizen environmental behavior. The results and conclusions are as follows. (1) The related research covered three periods: infancy, growth, and outbreak. (2) Chronologically, the relevant research evolved from building models to analyze the impact of environmental enrichment on human environmental behavior, to studying the motivation behind citizen environmental behavior, and finally to paying attention to sustainable development. (3) A cluster analysis of high-frequency keywords identified three research hotspots: “drivers of psychological distance of environmental change perception on citizen environmental behavior,” “impact of social distance on adolescents’ behavior,” and “construal level theory and citizen green behavior.” Based on these findings, possible future research directions were identified, including changing from a single theory to a combination of multiple theories to comprehensively study citizen environmental protection behavior; analyzing the motivation of citizen environmental behavior and summarizing the general motivation of environmental behavior according to its internal relationship; and determining how to narrow the global psychological distance, strengthen the awareness of the community of human destiny, and explore the establishment of an efficient global climate cooperation mechanism.
Moral Decision-Making During COVID-19: Moral Judgements, Moralisation, and Everyday Behaviour
Authors: Francis Kathryn B. , McNabb Carolyn B.
Published: February 2022

The COVID-19 pandemic continues to pose significant health, economic, and social challenges. Given that many of these challenges have moral relevance, the present studies investigate whether the COVID-19 pandemic is influencing moral decision-making and whether moralisation of behaviours specific to the crisis predict adherence to government-recommended behaviours. Whilst we find no evidence that utilitarian endorsements have changed during the pandemic at two separate timepoints, individuals have moralised non-compliant behaviours associated with the pandemic such as failing to physically distance themselves from others. Importantly, our findings show that this moralisation predicts sustained individual compliance with government-recommended behaviours.
Validation and Psychometric Properties of the Minnesota Living With Heart Failure Questionnaire in Individuals With Coronary Artery Disease in Lithuania
Authors: Gecaite-Stonciene Julija , Burkauskas Julius , Bunevicius Adomas , Steibliene Vesta , Macijauskiene Jurate , Brozaitiene Julija , Mickuviene Narseta , Kazukauskiene Nijole
Published: February 2022

Background

Health-related quality of life (HRQoL) is known to be impaired in individuals with coronary artery disease (CAD), especially in those after a recent acute coronary syndrome (ACS). Heart failure (HF) is a common burden in this population that significantly contributes to worsening HRQoL. To accurately measure the level of HRQoL in individuals with CAD after ACS, disease-specific scales, such as the Minnesota living with heart failure questionnaire (MLHFQ), are recommended. Nevertheless, to date, there has not been a study that would comprehensively evaluate the psychometric properties of the MLHFQ in a large sample of individuals with CAD after ACS. The debate regarding the internal structure of MLHFQ is also still present. Hence, this study aimed to translate the MLHFQ and evaluate its internal structure, reliability/precision, and validity in individuals with CAD following ACS in Lithuania.

Methods

In the cross-sectional study, 1,083 participants (70% men, age M = 58, SD = 9) were evaluated for sociodemographic and clinical characteristics. HRQoL was measured using the MLHFQ and the Short Form-36 health survey (SF-36). In addition, exercise capacity (EC) was also evaluated in the study patients, using a standardized computer-driven bicycle ergometer.

Results

The internal consistency of the MLHFQ subscales (0.79−0.88) was found to be good. Confirmatory factor analysis (CFA) provided the support for the three-factor model (“physical domain,” “social domain,” and “emotional domain”) of the MLHFQ and showed acceptable fit [comparative fit indices (CFI) = 0.894; goodness-of-fit (GFI) = 0.898; non-normal fit index (NFI) = 0.879, and root mean square error of approximation (RMSEA) = 0.073]. Regarding convergent evidence, significant associations were found between the MLHFQ domains and the SF-36 domains and EC (r’s range 0.11−0.58).

Conclusion

The current study completed cultural validation and provided further information on the psychometric characteristics of the MLHFQ in Lithuania, suggesting MLHFQ as a valid and reliable instrument to measure HRQoL. The Lithuanian version of MLHFQ is best described by a three-factor solution, measuring physical, social, and emotional dimensions of HRQoL among individuals with CAD following ACS.
Employee Perceptions About Participation in Decision-Making in the COVID Era and Its Impact on the Psychological Outcomes: A Case Study of a Cooperative in MONDRAGON (Basque Country, Spain)
Authors: Arregi Aitziber , Gago Monica , Legarra Maite
Published: February 2022

This research aims to study possible effects or impacts of COVID-19 in the context of a democratic organizational system analyzing how COVID-19 has influenced employees’ perception of their participation in decision-making and its impact on some psychological outcomes and emotions. COVID-19 has accelerated the process of implementation of new frameworks at work (digitalization, teleworking, new skills, and abilities) that have generated the modification of culture and employee management practices. Our hypothesis are, on the one hand, that COVID-19 has generated changes in participation structures and internal communication mechanisms, having to make modifications not to deteriorate the perception of employees about their participation in decision making. On the other hand, COVID-19 has generated changes in the psychological outcomes and emotions of the employees. In the study, we analyze a cooperative belonging to the MONDRAGON cooperative group, where participation in decision-making and ownership is in its DNA. Through qualitative (5 focus groups) and quantitative (short questionnaire) methodologies, involving 42 employees, we investigate firstly, how COVID-19 has affected perceptions about participation in decision-making analyzing what role has played internal communication in these perceptions. Secondly, we investigate how COVID-19 has affected psychological outcomes and emotions. In this case, the perceptions arising from participation in decision-making focus on the assessment that participators make of the governance channels and the day-to-day meetings. Therefore, their appropriateness seems to be a key factor in the perception of participation in the COVID-19 era. Differences have been detected between the perceptions of blue and white collar employees. Such differences have also been founded in the psychological outcomes and emotions. Although this is a single case study, the analysis carried out provides elements of reflection to modify and restructure the decision-making and participation mechanisms, adapting them to the needs of blue and white collar employees in order to “guarantee” the expected outcomes.
Risky Decision Making Under Stressful Conditions: Men and Women With Smaller Cortisol Elevations Make Riskier Social and Economic Decisions
Authors: Dreyer Anna J. , Stephen Dale , Human Robyn , Swanepoel Tarah L. , Adams Leanne , O'Neill Aimee , Jacobs W. Jake , Thomas Kevin G. F.
Published: February 2022

Men often make riskier decisions than women across a wide range of real-life behaviors. Whether this sex difference is accentuated, diminished, or stable under stressful conditions is, however, contested in the scientific literature. A critical blind spot lies amid this contestation: Most studies use standardized, laboratory-based, cognitive measures of decision making rather than complex real-life social simulation tasks to assess risk-related behavior. To address this blind spot, we investigated the effects of acute psychosocial stress on risk decision making in men and women (N = 80) using a standardized cognitive measure (the Iowa Gambling Task; IGT) and a novel task that simulated a real-life social situation (an online chatroom in which participants interacted with other men and women in sexually suggestive scenarios). Participants were exposed to either an acute psychosocial stressor or an equivalent control condition. Stressor-exposed participants were further characterized as high- or low-cortisol responders. Results confirmed that the experimental manipulation was effective. On the IGT, participants characterized as low-cortisol responders (as well as those in the Non-Stress group) made significantly riskier decisions than those characterized as high-cortisol responders. Similarly, in the online chatroom, participants characterized as low-cortisol responders (but not those characterized as high-cortisol responders) were, relative to those in the Non-Stress group, significantly more likely to make risky decisions. Together, these results suggest that at lower levels of cortisol both men and women tend to make riskier decisions in both economic and social spheres.
Involuntary Retirement and Depression Among Adults: A Systematic Review and Meta-Analysis of Longitudinal Studies
Authors: Zhai Long , Wang Junhui , Liu Yantao , Zhang Hua
Published: February 2022

Results from longitudinal studies on involuntary retirement and depression remain controversial. PubMed, Web of Science, Embase, ScienceDirect, Wanfang, and VIP updated on 4 January 2022 were searched for eligible publications. Pooled relative risks (RRs) with 95% confidence interval (CI) were calculated using a random-effects model. Eight published articles with 14,604 participants for the effect of involuntary retirement on depression incidence and 26,822 participants for the relationship between depression and involuntary retirement were included. Compared with working, the pooled RR for depression was 1.31 (95% CI, 1.13–1.51; I2 = 37.7%) for the involuntary retirement overall. For involuntary retirement, the pooled RR was 1.70 (95% CI, 1.28–2.25; I2 = 84.2%). The associations between involuntary retirement and depression did not substantially change in sensitivity and subgroup analyses. No evidence of publication bias was found. This meta-analysis indicates that there might be mutual causal relationship between involuntary retirement and depression. More large longitudinal studies with different gender and income levels are needed.
Interventions for the Current COVID-19 Pandemic: Frontline Workers' Intention to Use Personal Protective Equipment
Authors: Irfan Muhammad , Salem Sultan , Ahmad Munir , Acevedo-Duque Ángel , Abbasi Kashif Raza , Ahmad Fayyaz , Razzaq Asif , Işik Cem
Published: February 2022

Background

Frontline workers (FLWs) are at a higher risk of COVID-19 infection during care interactions than the general population. Personal protective equipment (PPE) is regarded as an effective intervention for limiting the transmission of airborne viruses. However, research examining FLWs' intention to use PPE is limited.

Objectives

This study addresses this research gap and also contributes by expanding the conceptual mechanism of planned behavior theory by incorporating three novel dimensions (perceived benefits of PPE, risk perceptions of the epidemic, and unavailability of PPE) in order to gain a better understanding of the factors that influence FLWs' intentions to use PPE.

Method

Analysis is based on a sample of 763 FLWs in Pakistan using a questionnaire survey, and the structural equation modeling approach is employed to evaluate the suppositions.

Results

Study results indicate that attitude, perceived benefits of PPE, and risk perceptions of the epidemic have positive influence on FLWs' intention to use PPE. In comparison, the unavailability of PPE and the cost of PPE have opposite effects. Meanwhile, environmental concern has a neutral effect.

Conclusions

The study results specify the importance of publicizing COVID-19's lethal impacts on the environment and society, ensuring cheap PPE, and simultaneously enhancing workplace safety standards.
The Pendulum Has Swung: How Do We Ensure a Life Course Approach to Immunisation in Australia?
Authors: Seale Holly , McFadden Kathleen , Dyda Amalie , Kaufman Jessica , Heywood Anita
Published: February 2022

Rather than concentrating primarily on children and adolescents, there has been a shift in the discourse around immunisation to encompass a whole-of-life approach. Despite this acknowledgement and ongoing high burdens of vaccine preventable diseases in adults, coverage for some adult risk groups remains sub-optimal. This study aimed to explore key informant's and stakeholder's perceptions of factors impacting provision of immunisation programs for Australian adults and to identify strategies to promote acceptance and uptake. Semi-structured telephone interviews were undertaken with people involved in adult immunisation program delivery, advocacy, policy or research between September 2020 and June 2021. Transcripts were inductively analysed, with the resulting themes categorised into the five influences on vaccination gaps that have informed program planning in other countries: Access, Affordability, Awareness, Acceptance and Activation. Participants spoke of improvements in the provision of vaccines to adults, however, ongoing challenges persisted. Participants agreed that the focus or emphasis of policies and the promotion/communication strategies has been on childhood vaccination in Australia, however there is a sense that the “pendulum has swung.” These included understanding of eligibility amongst the Australian population and the reluctance of some health providers to dedicate time to exploring immunisation needs with adult patients. In comparison to the childhood vaccination program, there has been a lack of data available on coverage for adult vaccines on the national immunisation program. This has contributed to the ongoing challenges of identifying and promoting certain vaccines. At a government level, questions were raised about why the Australian government has never set an aspirational target for adult vaccination (i.e., influenza or pneumococcal) coverage. While significant improvements have been made in adult immunisation uptake, there are still gaps across the program. While the system remains under stress because of the COVID-19 pandemic, it is not appropriate to implement any additional programs. There needs to be strong commitment to establish the value of adult vaccination in the eyes of community members, policy makers and healthcare professionals. Having a national adult immunisation strategic plan would help advance action.
Clinical Trials Targeting Aging
Authors: Nielsen Johannes Leth , Bakula Daniela , Scheibye-Knudsen Morten
Published: February 2022

The risk of morbidity and mortality increases exponentially with age. Chronic inflammation, accumulation of DNA damage, dysfunctional mitochondria, and increased senescent cell load are factors contributing to this. Mechanistic investigations have revealed specific pathways and processes which, proposedly, cause age-related phenotypes such as frailty, reduced physical resilience, and multi-morbidity. Among promising treatments alleviating the consequences of aging are caloric restriction and pharmacologically targeting longevity pathways such as the mechanistic target of rapamycin (mTOR), sirtuins, and anti-apoptotic pathways in senescent cells. Regulation of these pathways and processes has revealed significant health- and lifespan extending results in animal models. Nevertheless, it remains unclear if similar results translate to humans. A requirement of translation are the development of age- and morbidity associated biomarkers as longitudinal trials are difficult and not feasible, practical, nor ethical when human life span is the endpoint. Current biomarkers and the results of anti-aging intervention studies in humans will be covered within this paper. The future of clinical trials targeting aging may be phase 2 and 3 studies with larger populations if safety and tolerability of investigated medication continues not to be a hurdle for further investigations.
Persistent SARS-CoV-2 Infection in a Patient With Non-hodgkin Lymphoma: Intra-Host Genomic Diversity Analysis
Authors: Bianco Angelica , Capozzi Loredana , Del Sambro Laura , Simone Domenico , Pace Lorenzo , Rondinone Valeria , Difato Laura M. , Miccolupo Angela , Manzari Caterina , Fedele Alberto , Parisi Antonio
Published: February 2022

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic, threatening global public health. Several cases of persistent infection have been described, but there are few reports that compared the genetic variability among samples collected from the patient during infection. In the current study, we reported a viral genetic analysis of a diabetic male patient with Non-Hodgkin Lymphoma affected by persistent SARS-CoV-2 infection. We sequenced the patient-derived viral isolated both from oro/nasopharyngeal swab and VeroE6 cell line, collected from the same patient at different points of the infection. Due to the insufficient material of the second swab received, in order to obtain a complete coverage of the viral genome, it was convenient to perform a virus isolation after cell culture. Both genomes belonged to Pangolin Lineage B.1, Nextstrain clade 20A and GISAID clade G. The mutation spectrum predicted for the two viral genomes reveal three additionally mutations in the sequence of second sample when compared with mutations set identified in the first sample. Our findings show the evolution of the intra-host variability during the course of a long-lasting infection.
Measuring Health Literacy in Romania: Validation of the HLS-EU-Q16 Survey Questionnaire
Authors: Coman Madalina Adina , Forray Alina Ioana , Van den Broucke Stephan , Chereches Razvan Mircea
Published: February 2022

Objective:Empirical data on health literacy (HL) for Romania is almost inexistent. The present study aimed to validate the HLS-EU-Q16 questionnaire for the Romanian population and explore the predictors of HL in the North-West Region of the country.

Methods:A non-experimental, cross-sectional study was conducted between March and November 2019 on a representative, stratified random sample of 1,622 participants from the North-Western Region of Romania. Exploratory Factor Analysis (EFA), Principal component analysis (PCA), Pearson correlations, and Chronbach’s Alpha were used to validate the scale. Linear regressions were conducted to assess the determinants of health literacy.

Results:Results obtained for the HL scale support its factorial component and reliability, with a Cronbach’s alpha ofα= 0.84. Age, gender, education and self-reported health status were identified as determinants of HL.

Conclusion:Study findings indicate that the Romanian version of HLS-EU-Q16 is psychometrically sound and comparable to the original version. These results provide the first validated tool to measure HL in Romanian and the first population level data for Romania.
Donor-Transmitted Cancer in Orthotopic Solid Organ Transplant Recipients: A Systematic Review
Authors: Greenhall George H. B. , Ibrahim Maria , Dutta Utkarsh , Doree Carolyn , Brunskill Susan J. , Johnson Rachel J. , Tomlinson Laurie A. , Callaghan Chris J. , Watson Christopher J. E.
Published: February 2022

Donor-transmitted cancer (DTC) has major implications for the affected patient as well as other recipients of organs from the same donor. Unlike heterotopic transplant recipients, there may be limited treatment options for orthotopic transplant recipients with DTC. We systematically reviewed the evidence on DTC in orthotopic solid organ transplant recipients (SOTRs). We searched MEDLINE, EMBASE, PubMed, Scopus, and Web of Science in January 2020. We included cases where the outcome was reported and excluded donor-derived cancers. We assessed study quality using published checklists. Our domains of interest were presentation, time to diagnosis, cancer extent, management, and survival. There were 73 DTC cases in liver (n = 51), heart (n = 10), lung (n = 10) and multi-organ (n = 2) recipients from 58 publications. Study quality was variable. Median time to diagnosis was 8 months; 42% were widespread at diagnosis. Of 13 cases that underwent re-transplantation, three tumours recurred. Mortality was 75%; median survival 7 months. Survival was worst in transmitted melanoma and central nervous system tumours. The prognosis of DTC in orthotopic SOTRs is poor. Although re-transplantation offers the best chance of cure, some tumours still recur. Publication bias and clinical heterogeneity limit the available evidence. From our findings, we suggest refinements to clinical practice.

Systematic Review Registration:https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42020165001, Prospero Registration Number: CRD42020165001.
Relinquishing Anonymity in Living Donor Kidney Transplantation: Lessons Learned From the UK Policy for Anonymous Donors
Authors: Pronk Mathilde C. , Burnapp Lisa , Reinders Marlies E. J. , Massey Emma K.
Published: February 2022

Anonymous living donor kidney transplantation (LDKT) is performed in many countries and policies on anonymity differ. The UK is the only European country with a conditional policy, allowing pairs to break anonymity post-transplant. There is little evidence on how contact after anonymous LDKT is experienced. In this cross-sectional study participants who donated or received a kidney through non-directed altruistic kidney donation or within the UK living kidney sharing scheme completed a questionnaire on their experiences with and attitudes towards anonymity. Non-parametric statistics were used to analyse the data. 207 recipients and 354 donors participated. Anonymity was relinquished among 11% of recipients and 8% of donors. Non-anonymous participants were generally content with non-anonymity. They reported positive experiences with contact/meeting the other party. Participants who remained anonymous were content with anonymity, however, 38% would have liked to meet post-transplant. If the other party would like to meet, this number increased to 64%. Although participants agreed with anonymity before surgery, they believe that, if desired, a meeting should be allowed after surgery. UK donors and recipients were satisfied with conditional anonymity and experiences with breaking anonymity were positive. These results support the expansion of conditional anonymity to other countries that allow anonymous LDKT.
Investigation on Surface Roughness of PolyJet-Printed Airfoil Geometries for Small UAV Applications
Authors: Gülcan Orhan , Günaydın Kadir , Çelik Alican
Published: February 2022

The present study focuses on producibility and surface roughness characteristics of airfoil geometries and their effect on aerodynamic performance for different velocities, surface roughness values and angles of attack. Two different tray orientations (along X and Y axis), two different build directions (vertical and horizontal) and two different surface finish settings (matte and glossy) were used to evaluate the effect of these parameters on the surface roughness of both up- and down-facing surfaces of airfoils produced by PolyJet. On both surfaces, surface roughness measurements were performed on two crossing directions. The results showed that horizontal build direction where surfaces of airfoils were parallel to the build platform experienced lower surface roughness than the vertical build direction. Vertically oriented specimens showed a considerable degree of distortions especially in trailing edges along with very high surface irregularities on side walls. In general, glossy or matte finish settings resulted in similar surface roughness values and specimens located along X direction showed better surface quality than specimens located along Y direction with an inconsiderable difference. Besides this, CFD analysis revealed that surface roughness caused by printing strategies directly influences the aerodynamic performance of the fixed-wing UAVs (Unmanned Aerial Vehicles) to considerable degrees. The increase in the drag force coefficient, due to surface roughness, reached almost 7.5% for high cruise velocity at 0° angle of attack and 13% at 10° angle of attack in which stall commences.
The Effect of Climatic Parameters on Strawberry Production in a Small Walk-In Greenhouse
Authors: Khammayom Napassawan , Maruyama Naoki , Chaichana Chatchawan
Published: February 2022

The purpose of this study was to evaluate the impact of different environmental factors such as temperature, solar radiation, and relative humidity on the quality of strawberries in terms of their shape, size, and sugar accumulation. The experiment was carried out in a small walk-in greenhouse in Matsusaka city, Japan. Harunoka strawberries (Fragaria × ananassa Duch.) were cultivated from September to May of the following year. Production was evaluated on 20 February 2021 (peak season) and 5 April 2021 (end season). To evaluate the influence of environmental factors on strawberry fruit quality, the weight, shape, and soluble sugar content were recorded and compared to each other. According to the environmental data, the average temperature between day and night at peak harvest was around 12 °C, which was suitable for high-quality strawberry cultivation. However, the average temperature difference between day and night was approximately 4 °C at the end of the season. In addition, there were no significant differences in solar radiation and relative humidity between both seasons. Increasing temperatures led to the decline in the soluble sugar content at the end season. Thus, it can be concluded that the temperature difference between day and night is a major factor affecting strawberry production. The assessment of the impact of environmental conditions on strawberry quality can be used as a guideline not only in temperate climates, but also in other climates, such as in tropical countries.
A Bioinformatics Approach to Mine the Microbial Proteomic Profile of COVID-19 Mass Spectrometry Data
Authors: Alnakli Aziz Abdullah A. , Jabeen Amara , Chakraborty Rajdeep , Mohamedali Abidali , Ranganathan Shoba
Published: February 2022

Mass spectrometry (MS) is one of the key technologies used in proteomics. The majority of studies carried out using proteomics have focused on identifying proteins in biological samples such as human plasma to pin down prognostic or diagnostic biomarkers associated with particular conditions or diseases. This study aims to quantify microbial (viral and bacterial) proteins in healthy human plasma. MS data of healthy human plasma were searched against the complete proteomes of all available viruses and bacteria. With this baseline established, the same strategy was applied to characterize the metaproteomic profile of different SARS-CoV-2 disease stages in the plasma of patients. Two SARS-CoV-2 proteins were detected with a high confidence and could serve as the early markers of SARS-CoV-2 infection. The complete bacterial and viral protein content in SARS-CoV-2 samples was compared for the different disease stages. The number of viral proteins was found to increase significantly with the progression of the infection, at the expense of bacterial proteins. This strategy can be extended to aid in the development of early diagnostic tests for other infectious diseases based on the presence of microbial biomarkers in human plasma samples.