bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Sex on Infection Risk in Patients with Systemic Lupus Erythematosus
Authors: Borrelli R. , Nicola S. , Corradi F. , Badiu I. , Lo Sardo L. , Rashidy N. , Quinternetto A. , Mazzola M. , Meli F. , Saracco E. , Vitali I. , Negrini S. , Brussino L.
Published: January 2025

Background: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that exhibits considerable diversity in terms of both clinical and immunological manifestations. Since its female-to-male ratio is around 9:1, it is well recognized that systemic lupus erythematosus mostly affects women, especially those of childbearing age. There is a greater susceptibility to infections in adult patients with systemic lupus erythematosus (SLE) compared to the general population. However, only a small number of studies have attempted to analyze this risk using real-life data, and even fewer have successfully assessed the influence of sex. Materials and Methods: A retrospective study was conducted, enrolling patients and dividing them into two groups based on their biological sex. Infectious episodes were identified from medical records and categorized by severity. Patients were stratified according to disease duration and treatment received. Logistic regression analysis was used to calculate the odds ratio (OR), with a 95% confidence interval (CI) for the assessment of risk factors. Multivariable logistic regression was performed to adjust for potential confounders. Model fit was evaluated using the Hosmer–Lemeshow test, and interactions between variables were tested. Sensitivity analyses were conducted to assess the robustness of the findings. Results: A total of 119 patients (107 females and 12 males) were included in the analysis. No significant difference in age was found between sexes (t = −0.715, p = 0.487), but disease duration was significantly shorter in males (t = 3.35, p = 0.003). Logistic regression showed a significant association between male sex and infection risk (β = 0.9426, p = 0.05), with males having an almost sixfold higher probability of infection compared to females (OR 5.675, 95% CI: 1.4479–22.2477, p = 0.0127). Disease duration (β = 0.0250, p = 0.102) and smoking status (β = 0.4529, p = 0.078) were not statistically significant. Lastly, correlation analysis revealed a significant association between SS-A antibodies and infection rate (r = 0.291, p = 0.003). Conclusions: This study highlights a significant sex-based disparity in the risk of infections among SLE patients, with males being at a higher risk compared to females. The differences in the distribution of infections, such as the higher prevalence of pneumonia in males and urinary tract infections in females, suggest that sex-specific factors, including immunological and hormonal differences, may influence infection susceptibility. Our findings emphasize the need for tailored clinical management, with increased vigilance for infections in male patients, to improve prevention strategies and targeted therapeutic interventions in this subgroup.
iPSC Technology Revolutionizes CAR-T Cell Therapy for Cancer Treatment
Authors: Zong Jiepu , Li Yan-Ruide
Published: January 2025

Chimeric Antigen Receptor (CAR)-engineered T (CAR-T) cell therapy represents a highly promising modality within the domain of cancer treatment. CAR-T cell therapy has demonstrated notable efficacy in the treatment of hematological malignancies, solid tumors, and various infectious diseases. However, current CAR-T cell therapy is autologous, which presents challenges related to high costs, time-consuming manufacturing processes, and the necessity for careful patient selection. A potential resolution to this restriction could be found by synergizing CAR-T technology with the induced pluripotent stem cell (iPSC) technology. iPSC technology has the inherent capability to furnish an inexhaustible reservoir of T cell resources. Experimental evidence has demonstrated the successful generation of various human CAR-T cells using iPSC technology, showcasing high yield, purity, robustness, and promising tumor-killing efficacy. Importantly, this technology enables the production of clinical-grade CAR-T cells, significantly reducing manufacturing costs and time, and facilitating their use as allogeneic cell therapies to treat multiple cancer patients simultaneously. In this review, we aim to elucidate essential facets of current cancer therapy, delineate its utility, enumerate its advantages and drawbacks, and offer an in-depth evaluation of a novel and pragmatic approach to cancer treatment.
Accessible AI Diagnostics and Lightweight Brain Tumor Detection on Medical Edge Devices
Authors: Abdusalomov Akmalbek , Mirzakhalilov Sanjar , Umirzakova Sabina , Shavkatovich Buriboev Abror , Meliboev Azizjon , Muminov Bahodir , Jeon Heung Seok
Published: January 2025

The timely and accurate detection of brain tumors is crucial for effective medical intervention, especially in resource-constrained settings. This study proposes a lightweight and efficient RetinaNet variant tailored for medical edge device deployment. The model reduces computational overhead while maintaining high detection accuracy by replacing the computationally intensive ResNet backbone with MobileNet and leveraging depthwise separable convolutions. The modified RetinaNet achieves an average precision (AP) of 32.1, surpassing state-of-the-art models in small tumor detection (APS: 14.3) and large tumor localization (APL: 49.7). Furthermore, the model significantly reduces computational costs, making real-time analysis feasible on low-power hardware. Clinical relevance is a key focus of this work. The proposed model addresses the diagnostic challenges of small, variable-sized tumors often overlooked by existing methods. Its lightweight architecture enables accurate and timely tumor localization on portable devices, bridging the gap in diagnostic accessibility for underserved regions. Extensive experiments on the BRATS dataset demonstrate the model robustness across tumor sizes and configurations, with confidence scores consistently exceeding 81%. This advancement holds the potential for improving early tumor detection, particularly in remote areas lacking advanced medical infrastructure, thereby contributing to better patient outcomes and broader accessibility to AI-driven diagnostic tools.
Bioprospecting of a Native Plant Growth-Promoting Bacterium Bacillus cereus B6 for Enhancing Uranium Accumulation by Sudan Grass (Sorghum sudanense (Piper) Stapf)
Authors: Wu Longyuan , Zhang Lijuan , Wang Ning , Huang Wei , Wang Yanzhi , Sun Meng , Zheng Guofeng , Wang Wei , Shi Chong
Published: January 2025

Phytoremediation technology is viewed as a potential solution for addressing soil uranium contamination. Sudan grass (Sorghum sudanense (Piper) Stapf.), noted for its robust root structure and resilience to heavy metals, has garnered significant attention. This paper investigates a strain of uranium-tolerant bacterium, B6, obtained from the inter-root environment of native plants in soil contaminated with uranium tailings. The bacterium was identified as Bacillus cereus. Genomic analyses and assessment of uranium tolerance-promoting properties showed that strain B6 not only exhibited high uranium tolerance, but also possessed beneficial properties such as phosphorus solubilization and iron-producing carriers. In this study, we used strain B6 as an inoculant in combination with Sudan grass for germination and potting experiments. The findings demonstrated that Bacillus cereus B6 could substantially mitigate the adverse effects of uranium stress on Sudan grass, boost the plant’s antioxidant response, significantly increase the root length and dry biomass of Sudan grass, and facilitate the accumulation of uranium in the roots, as well as its translocation to the aboveground portions. The study showed that PGPB strain B6 can significantly enhance the effect of plant accumulation of uranium and increase the potential of Sudan grass to become a uranium-rich plant, which provides an important scientific basis and application prospect for the use of microbial-assisted Sudan grass remediation technology to treat uranium-contaminated soil.
Microbial Contamination and Food Safety (Volume I)
Authors: Barbosa Joana Bastos
Published: January 2025

Food safety is a global concern driven by the constant need to find innovative solutions to mitigate contamination risks by pathogenic microorganisms [...]
Research Progress on Natural Products That Regulate miRNAs in the Treatment of Osteosarcoma
Authors: Wang Lin , Liu Xinyu , Lv Haoze , Zhang Han , Lin Rimei , Xu Shan , Zhang Chaojing , Lou Shilei , Qiu Zhidong , Sun Cong , Cui Ning
Published: January 2025

miRNAs are small non-coding RNA molecules that play critical roles in the regulation of gene expression and have been closely associated with various diseases, including cancer. These molecules significantly influence the cell cycle of tumor cells and control programmed cell death (apoptosis). Currently, research on miRNAs has become a major focus in developing cancer therapies. Osteosarcoma, a malignant neoplasm predominantly occurring during adolescence and later in life, is characterized by a high propensity for metastasis. This review explores the role of miRNAs in the initiation and progression of cancer, highlighting their potential as predictive biomarkers for disease. It discusses the mechanisms by which natural products modulate miRNA activity to influence apoptosis, ferroptosis, and autophagy in osteosarcoma cells, aiming to identify new strategies for osteosarcoma treatment. Recent studies on how natural products regulate miRNAs to reduce tumor cell resistance to chemotherapy are also reviewed. Furthermore, the review elaborates on how natural products regulate m6A modifications to influence miRNA expression, thereby exerting antitumor effects. In this process, interactions between m6A modifications and miRNAs have been identified, with both jointly influencing tumorigenesis and cancer progression, offering a new perspective in osteosarcoma treatment. These approaches could help uncover novel regulatory mechanisms in osteosarcoma pathways and provide a theoretical foundation for developing new drugs and identifying novel therapeutic targets.
Anti-Tissue-Transglutaminase IgA Antibodies Presence Determination Using Electrochemical Square Wave Voltammetry and Modified Electrodes Based on Polypyrrole and Quantum Dots
Authors: Pãun Angela Gabriela , Popescu Simona , Ungureanu Alisa Ioana , Trusca Roxana , Popp Alina , Dumitriu Cristina , Buica George-Octavian
Published: January 2025

A novel electrochemical detection method utilizing a cost-effective hybrid-modified electrode has been established. A glassy carbon (GC) modified electrode was tested for its ability to measure electrochemical tTG antibody levels, which are essential for diagnosing and monitoring Celiac disease (CD). Tissue transglutaminase protein biomolecules are immobilized on a quantum dots-polypyrrole nanocomposite in the improved electrode. Initial, quantum dots (QDs) were obtained from Bombyx mori silk fibroin and embedded in polypyrrole film. Using carbodiimide coupling, a polyamidoamine (PAMAM) dendrimer was linked with GQDs-polypyrrole film to improve sensor sensitivity. The tissue transglutaminase (tTG) antigen was cross-linked onto PAMAM using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC)-N-hydroxy succinimide (NHS) chemistry to develop a nanoprobe that can detect human serum anti-tTG antibodies. The physicochemical characteristics of the synthesized nanocomposite were examined by FTIR, UV-visible, FE-SEM, EDX, and electrochemical studies. The novel electrode measures anti-tissue antibody levels in real time using human blood serum samples. The modified electrode has great repeatability and an 8.7 U/mL detection limit. Serum samples from healthy people and CD patients were compared to standard ELISA kit assays. SPSS and Excel were used for statistical analysis. The improved electrode and detection system can identify anti-tissue antibodies up to 80 U/mL.
Assessing the Measurement Properties of the Test of Gross Motor Development-3 Using the COSMIN Methodology—A Systematic Review
Authors: Zhu Yuanye , Wang Jing , Ding Yaru , Qian Yongdong , Korivi Mallikarjuna , Chen Qian , Ye Weibing
Published: January 2025

This study aimed to systematically review the measurement properties of the Test of Gross Motor Development-3 (TGMD-3) using the COSMIN methodology. A search of four databases (PubMed, EMBASE, Web of Science, CINAHL) identified 23 relevant studies. The methodological quality of the studies was assessed using the COSMIN risk of bias checklist; the measurement properties of the TGMD-3 were evaluated by the COSMIN quality criteria; and the quality of the evidence was rated using a modified GRADE approach. The findings indicated that the test–retest, inter-rater, and intra-rater reliability, as well as measurement invariance and part content validity (relevance and comprehensibility), were sufficient, supported by high-quality evidence. The bifactor structure was found to be a more appropriate model for the TGMD-3, with structural validity and internal consistency rated as sufficient, though based on moderate-quality evidence. However, hypothesis testing for construct validity produced inconsistent results, also supported by moderate-quality evidence. Responsiveness was rated as inconsistent, based on low-quality evidence. Overall, the TGMD-3 is graded as “B”, meaning it has the potential to be recommended, but further research is needed to fully establish its measurement properties. Future studies should focus on verifying the comprehensiveness of items of the TGMD-3 to optimise its application.
Optimizing Public Spaces for Age-Friendly Living: Renovation Strategies for 1980s Residential Communities in Hangzhou, China
Authors: Gong Min , Wang Ning , Chu Yubei , Wu Yiyao , Huang Jiadi , Wu Jing
Published: January 2025

Population aging and urbanization are two of the most significant social transformations of the 21st century. Against the backdrop of rapid aging in China, developing age-friendly community environments, particularly through the renovation of legacy residential communities, not only supports active and healthy aging but also promotes equity and sustainable development. This study focuses on residential communities built in the 1980s in Hangzhou, exploring strategies for the age-friendly renovation of outdoor public spaces. The residential communities that flourished during the construction boom of the 1980s are now confronting a dual challenge: aging populations and deteriorating facilities. However, existing renovation efforts often pay insufficient attention to the comprehensive age-friendly transformation of outdoor public spaces within these neighborhoods. Following a structured research framework encompassing investigation, evaluation, design, and discussion, this study first analyzes linear grid layouts and usage patterns of these communities. Then, the research team uses post-occupancy evaluation (POE) to assess the age-friendliness of outdoor public spaces. Semi-structured interviews with elderly residents identify key concerns and establish a preliminary evaluation framework, while a Likert-scale questionnaire quantifies the satisfaction with age-friendly features across four communities. The assessment reveals that key age-friendliness issues, including poor traffic safety, dispersed activity spaces, and insufficiently adapted facilities, are closely linked to the linear usage patterns within the spatial framework of the grid layouts. Based on the findings, the study develops tiered renovation goals, renovation principles and implemented an age-friendly design in the Hemu Community. The strengths, weaknesses, and feasibility of the renovation plan are discussed, while three recommendations are made to ensure successful implementation. The study is intended to provide a valuable reference for advancing age-friendly residential renewal efforts in Hangzhou and contributing to the broader objective of sustainable, inclusive city development.
A Study of Member Displacement According to Seasonal Climate of the Sungnyemun Gate, a Korean Wooden Architectural Heritage Site
Authors: Seo Hyowon , Lee Hana , Kim Sunghan
Published: January 2025

This study analyzes the results of a displacement measurement of the Sungnyemun Gate’s structural members, such as column, girder, and hip rafter, carried out by the National Institute of Cultural Heritage for about 10 years from December 2013 to October 2022. Through this, we attempt to examine the behavior of wooden architectural heritage sites according to seasonal changes and infer the factors influencing structural deformation. As a result of the analysis, it was confirmed that the structural members of the Sungnyemun Gate, including the columns, girders, and hip rafters, continued to move and that the displacement of members was accumulated, and the structure was deformed. It was also confirmed that member displacements accumulated in a specific direction. In the case of the Sungnyemun Gate, the column leaning south, the hip rafters’ endpoint sagging, and the girders’ center deflecting were continuously observed. Furthermore, the behavior of wooden architectural heritage sites, where displacement accumulates as it undergoes repeated deformation and recovery according to seasonal changes, was also revealed in detail. The deformation of the Sungnyemun Gate’s members shows a pattern that reflects the mechanical properties of wood, which repeatedly increases and decreases displacement depending on the season. However, seasonal deformation did not appear the same in all the members. Even the same member has an uneven drying speed due to differences in the amount of sunlight it receives depending on its location, which leads to uneven distribution of deformation. The significance of this study is that it examined the behavior of a wooden architectural heritage site in detail based on the quantitative results of long-term measurements and prepared primary data for the future management of wooden architectural heritage sites.
Research on Concrete Beam Damage Detection Using Convolutional Neural Networks and Vibrations from ABAQUS Models and Computer Vision
Authors: Bai Xin , Zhang Zi
Published: January 2025

Researchers have already used vibration data and deep learning methods, such as Convolutional Neural Networks (CNNs), to detect structural damage. Moreover, some researchers have employed image-based displacement sensors (such as the template matching and edge detection methods) to obtain structural vibration information. It is necessary to verify whether deep learning methods can detect minor damage inside beams, for example, small hollowing in concrete. In addition, there is an urgent need to develop an effective image-based displacement sensor that can simultaneously detect a large number of reliable vibration data from different measurement points. In this study, the vibration data of two beam-ABAQUS models were used as the input data for a newly designed deep learning-based structural health monitoring method. There were 500 vibration samples for each case, and the peak of vibrations was several millimeters. The proposed CNN model can locate damage positions in beams with high accuracy (close to 100%), and the damage sizes are 3 cm and 6 cm. Laboratory experiments were carried out on four beams with different damage. The optimized displacement sensor developed based on the edge detection method was used to detect the displacement of the beams. Each beam had 200 vibration data, and there were 800 vibration data in total. These vibration data were used as input data to train the proposed deep learning architecture, and satisfactory accuracy was achieved in detecting the damage of the beams with an accuracy of 97%. The training process is satisfactory in that the training loss and validation loss dropped very quickly.
A Multidisciplinary Approach to Diagnosing Blastic Plasmacytoid Dendritic Cell Neoplasm (BPDCN): Practical Recommendations and Insights from Countries of the Gulf Cooperation Council
Authors: Bakshi Nasir , Al Hejazi Ayman , Al-Maghraby Hatim , Al Mugairi Areej , Alotaibi Ahmad S. , Khogeer Haitham , Seliem Rania Medhat , Pandita Ramesh , Raslan Heba , Aung Phyu P. , Ohgami Robert S.
Published: January 2025

Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is an aggressive orphan hematopoietic malignancy characterized by cutaneous and systemic hematologic involvement. BPDCN is frequently misidentified, but early, accurate diagnosis is critical to extending patient survival using tagraxofusp, a first-in-class CD123-targeted therapy, and increasing their chances of receiving a potentially curative stem cell transplantation. Cases of BPDCN in countries of the Gulf Cooperation Council are lower than the extrapolated incidence from other geographic locations due to lack of awareness of key diagnostic features, lack of consensus on the minimal phenotype for diagnosis, and lack of local immunohistochemistry testing facilities, contributing to underdiagnosis in this region. Practical recommendations, a streamlined diagnostic panel, and suggested multidisciplinary approaches based on expert experience regarding diagnostic and clinical challenges specific to this region, and a review of the literature are presented here to facilitate diagnosis of BPDCN in this region by primary care physicians, dermatologists, and hematologists.
Unveiling the Interplay Between the Human Microbiome and Gastric Cancer: A Review of the Complex Relationships and Therapeutic Avenues
Authors: Al-Matouq Jenan , Al-Ghafli Hawra , Alibrahim Noura N. , Alsaffar Nida , Radwan Zaheda , Ali Mohammad Daud
Published: January 2025

The human microbiota plays a crucial role in maintaining overall health and well-being. The gut microbiota has been implicated in developing and progressing various diseases, including cancer. This review highlights the related mechanisms and the compositions that influence cancer pathogenesis with a highlight on gastric cancer. We provide a comprehensive overview of the mechanisms by which the microbiome influences cancer development, progression, and response to treatment, with a focus on identifying potential biomarkers for early detection, prevention strategies, and novel therapeutic interventions that leverage microbiome modulation. This comprehensive review can guide future research and clinical practices in understanding and harnessing the microbiome to optimize gastric cancer therapies.
Triple-Negative Breast Cancer Progression and Drug Resistance in the Context of Epithelial–Mesenchymal Transition
Authors: Błaszczak Ewa , Miziak Paulina , Odrzywolski Adrian , Baran Marzena , Gumbarewicz Ewelina , Stepulak Andrzej
Published: January 2025

Triple-negative breast cancer (TNBC) is one of the most difficult subtypes of breast cancer to treat due to its distinct clinical and molecular characteristics. Patients with TNBC face a high recurrence rate, an increased risk of metastasis, and lower overall survival compared to other breast cancer subtypes. Despite advancements in targeted therapies, traditional chemotherapy (primarily using platinum compounds and taxanes) continues to be the standard treatment for TNBC, often with limited long-term efficacy. TNBC tumors are heterogeneous, displaying a diverse mutation profile and considerable chromosomal instability, which complicates therapeutic interventions. The development of chemoresistance in TNBC is frequently associated with the process of epithelial–mesenchymal transition (EMT), during which epithelial tumor cells acquire a mesenchymal-like phenotype. This shift enhances metastatic potential, while simultaneously reducing the effectiveness of standard chemotherapeutics. It has also been suggested that EMT plays a central role in the development of cancer stem cells. Hence, there is growing interest in exploring small-molecule inhibitors that target the EMT process as a future strategy for overcoming resistance and improving outcomes for patients with TNBC. This review focuses on the progression and drug resistance of TNBC with an emphasis on the role of EMT in these processes. We present TNBC-specific and EMT-related molecular features, key EMT protein markers, and various signaling pathways involved. We also discuss other important mechanisms and factors related to chemoresistance in TNBC within the context of EMT, highlighting treatment advancements to improve patients’ outcomes.
Next-Generation Immunotherapy for Hepatocellular Carcinoma: Mechanisms of Resistance and Novel Treatment Approaches
Authors: Eghbali Shabnam , Heumann Thatcher Ross
Published: January 2025

Hepatocellular carcinoma (HCC) is a leading cause of cancer-related mortality worldwide, and, with only 15–20% of HCC patients being suitable for potentially curative treatments, the vast majority of patients with HCC ultimately require systemic therapy. For decades, the choice of effective systemic therapy for HCC remained sparse. In recent years, after the combination of atezolizumab and bevacizumab demonstrated superior overall survival over the first-line standard, sorafenib, there has been a major therapeutic paradigm shift to immunotherapy-based regimens for HCC. While representing a great leap forward for the treatment of this cancer, the reality is that less than one-third of patients achieve an objective response to immune checkpoint inhibitor-based therapy, so there remains a significant clinical need for further therapeutic optimization. In this review, we provide an overview of the current landscape of immunotherapy for unresectable HCC and delve into the tumor intrinsic and extrinsic mechanisms of resistance to established immunotherapies with a focus on novel therapeutic targets with strong translational potential. Following this, we spotlight emerging immunotherapy approaches and notable clinical trials aiming to optimize immunotherapy efficacy in HCC that include novel immune checkpoint inhibitors, tumor microenvironment modulators, targeted delivery systems, and locoregional interventions.
Female Oncofertility and Immune Checkpoint Blockade in Melanoma: Where Are We Today?
Authors: Lee Cha Len , Martinez Erika , Malon Gimenez Diego , Muniz Thiago Pimentel , Butler Marcus Otho , Saibil Samuel David
Published: January 2025

The incidence of melanoma among young adults has risen, yet mortality has declined annually since the introduction of immune checkpoint inhibitors (ICI). The utilization of peri-operative ICI has significantly altered the treatment landscape in melanoma, with PD-1 inhibitors showing promising efficacy in improving relapse-free survival rates in high-risk stage II-III disease. With the increasing use of ICI, secondary concerns have emerged regarding the impact of cancer drugs on fertility and reproductive health among women of childbearing potential, especially in early-stage cancer settings. The exclusion of pregnant women from trials contributes to limited human data and clinical uncertainties, such as maternal and fetal toxicities related to ICI exposure during pregnancy, as well as the value of fertility preservation before ICI therapy. Uncertainty persists regarding pregnancy post-adjuvant immunotherapy, given the potential detrimental effects of hormonal and immunological changes during pregnancy on melanoma relapse. There is additional uncertainty about whether pregnancy-associated melanoma (PAM) represents a distinct disease entity that warrants tailored management compared to non-pregnant cases. Our review aims to give an overview of oncofertility practices among female melanoma patients after immunotherapy. We also focus on the literature gap in the published evidence and synthesize summaries regarding ICI toxicities on reproductive health and fetal development, pregnancy planning, and recurrence risks after melanoma treatment.
Characterization of a 3S PRAME VLD-Specific T Cell Receptor and Its Use in Investigational Medicinal Products for TCR-T Therapy of Patients with Myeloid Malignancies
Authors: Bürdek Maja , Prinz Petra U. , Mutze Kathrin , Tippmer Stefanie , Geiger Christiane , Longinotti Giulia , Schendel Dolores J.
Published: January 2025

Background/Objectives: MDG1011 is an autologous TCR-T therapy developed as a treatment option for patients with myeloid malignancies, including acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and multiple myeloma (MM). It is specific for the target antigen PReferentially expressed Antigen in MElanoma (PRAME). The recombinant TCR used in MDG1011 recognizes PRAME100–108 VLD-peptide presented by HLA-A*02:01-encoded surface molecules. Methods: Two preclinical batches of MDG1011, produced from enriched CD8+ T cells of healthy donors, underwent rigorous evaluation of on-target and off-target recognition of tumor cells and test cells representing healthy tissues. MDG1011 investigational medicinal products (IMPs) were produced for 13 patients. VLD-TCR surface expression was assessed using dual-marker flow cytometry using TCR V-beta-specific antibody and VLD/HLA-A2-specific multimer. Functionality was assessed by interferon-gamma (IFN-γ) secretion and cell-mediated cytotoxicity of target cells. Results: Preclinical MDG1011 batches displayed strong VLD-TCR expression, cytokine secretion, and cytotoxicity after antigen-specific activation, while showing no signals of on-target/off-tumor or off-target recognition. All IMPs had good VLD-TCR expression as well as functionality after activation by multiple target cells. Conclusions: Preclinical studies demonstrated that MDG1011 displayed key 3S attributes of high specificity, sensitivity, and safety required for regulatory approval of a first-in-human (FIH) clinical study of patients with myeloid malignancies (CD-TCR-001: ClinicalTrials.gov Identifier: NCT03503968). MDG1011 IMP manufacturing was successful at 92%, even including heavily pretreated elderly patients with very advanced disease. The IMPs applied in nine patients all displayed antigen-specific functionality. Elsewhere, clinical study results for MDG1011 showed no dose-limiting toxicity and signs of biological and/or clinical activity in several patients.
Hydrogenation of Simulated Bio-Syngas in the Presence of GdBO3 (B = Fe, Co, Mn) Perovskite-Type Oxides
Authors: Sheshko Tatiana F. , Akhmina Polina V. , Skvortsova Liliya G. , Borodina Elizaveta M. , Kryuchkova Tatiana A. , Zvereva Irina A. , Cherednichenko Alexander G.
Published: January 2025

Direct light olefin synthesis from bio-syngas hydrogenation is a promising pathway to decarbonize the chemical industry. The present study is devoted to the investigation of co-hydrogenation of carbon oxides in the presence of complex systems with the perovskite structure GdBO3 (B = Fe, Mn, Co). The catalyst samples were synthesized by sol-gel technology and characterized by XRD, XPS, BET and TPR. It was found that the Fe/Mn-containing samples exhibited efficient catalysis of the hydrogenation of simulated bio-syngas to light hydrocarbons. The GdMnO3 catalyst exhibits selectivity for C2–C3 light olefins of up to 37% among C1+ hydrocarbons, with a maximum olefin/paraffin ratio. GdMnO3 also exhibits high conversion of CO and CO2, reaching up to 70–75% at 723 K. However, the GdFeO3 catalyst shows a lower selectivity of (C2−3= = 22%, while it exhibits a higher conversion of CO2, up to 95%, at the same temperature. Herein, we established a catalyst structure–performance relationship as a function of chemical composition. Oxygen mobilities and ratios of surface (Os) to lattice (Ol) oxygen, forms of hydrogen adsorption, formation of -CHx- radicals and their subsequent recombination to olefins are influenced by the nature of the element in the B position. This work provides valuable insights for the rational design of bimetallic catalysts for bio-syngas hydrogenation.
Solid-State Reaction Synthesis of CoSb2O6-Based Electrodes Towards Oxygen Evolution Reaction in Acidic Electrolytes: Effects of Calcination Time and Temperature
Authors: Vanzetti Francesco , Guzmán Hilmar , Hernández Simelys
Published: January 2025

Mitigating global warming necessitates transitioning from fossil fuels to alternative energy carriers like hydrogen. Efficient hydrogen production via electrocatalysis requires high-performance, stable anode materials for the oxygen evolution reaction (OER) to support the hydrogen evolution reaction (HER) at the cathode. Developing noble metal-free electrocatalysts is therefore crucial, particularly for acidic electrolytes, to avoid reliance on scarce and expensive metals such as Ir and Ru. This study investigates a low-cost, solvent-free solid-state synthesis of CoSb2O6, focusing on the influence of calcination time and temperature. Six samples were prepared and characterized using powder X-ray diffraction (PXRD), energy-dispersive X-ray spectroscopy (EDX), Brunauer–Emmett–Teller (BET) analysis, field-emission scanning electron microscopy (FESEM), and electrochemical techniques. A non-pure CoSb2O6 phase was observed across all samples. Electrochemical testing revealed good short-term stability; however, all samples exhibited Tafel slopes exceeding 200 mV dec−1 and overpotentials greater than 1 V. The sample calcined at 600 °C for 6 h showed the best performance, with the lowest Tafel slope and overpotential, attributed to its high CoSb2O6 content and maximized {110} facet exposure. This work highlights the role of calcination protocols in developing Co-based OER catalysts and offers insights for enhancing their electrocatalytic properties.
Glucose-Sensing Carbohydrate Response Element-Binding Protein in the Pathogenesis of Diabetic Retinopathy
Authors: Starr Christopher R. , Zhylkibayev Assylbek , Gorbatyuk Oleg , Nuotio-Antar Alli M. , Mobley James , Grant Maria B. , Gorbatyuk Marina
Published: January 2025

Glucose-sensing ChREBP and MondoA are transcriptional factors involved in the lipogenic, inflammatory, and insulin signaling pathways implicated in metabolic disorders; however, limited ocular studies have been conducted on these proteins. We aimed to investigate the potential role of ChREBP in the pathogenesis of diabetic retinopathy (DR). We used diabetic human and mouse retinal cryosections analyzed by immunohistochemistry. qRT-PCR was performed to quantify gene expression. To explore the role of ChREBP in rods, we generated caChREBPRP mice with constitutively active (ca) ChREBP. These mice underwent retinal functional testing, which was followed by proteomic analysis using LC-MS. Furthermore, ARPE-19 cells were infected with lentiviral particles expressing human ChREBP (ARPE-19ChREBP) and subjected to global proteomics. Our results demonstrate that both proteins were expressed across the retina, although with distinct distribution patterns: MondoA was more prominently expressed in cones, while ChREBP was broadly expressed throughout the retina. Elevated expression of both proteins was observed in DR. This may have contributed to rod photoreceptor degeneration, as we observed diminished scotopic ERG amplitudes in caChREBPRP mice at P35. The retinal proteomic landscape revealed a decline in the KEGG pathways associated with phototransduction, amino acid metabolism, and cell adhesion. Furthermore, rod-specific caChREBP induced TXNIP expression. Consistent with altered retinal proteomics, ARPE-19ChREBP cells exhibit a metabolic shift toward increased glyoxylate signaling, sugar metabolism, and lysosomal activation. Our study demonstrates that ChREBP overexpression causes significant metabolic reprogramming triggering retinal functional loss in mice.
Blockade of TIPE2-Mediated Ferroptosis of Myeloid-Derived Suppressor Cells Achieves the Full Potential of Combinatory Ferroptosis and Anti-PD-L1 Cancer Immunotherapy
Authors: Tariq Hafiza Kashaf , Liang Zihao , Rabiu Lawan , Ibrahim Abdulrahman , Mohamady Farouk Abdalsalam Nada , Li Rong , Yang Qiong , Wan Xiaochun , Yan Dehong
Published: January 2025

Although immune checkpoint blockade (ICB) therapy has attained unprecedented clinical success, the tolerance and immune suppression mechanisms evolved by tumor cells and their tumor microenvironment (TME) hinder its maximum anti-cancer potential. Ferroptosis therapy can partially improve the efficacy of ICB, but it is still subject to immune suppression by myeloid-derived suppressor cells (MDSCs) in the TME. Recent research suggests that an MDSC blockade can unleash the full therapeutic potential of the combined therapy of ferroptosis and ICB in liver cancer treatment. However, whether blocking the intrinsic ferroptosis pathways of MDSCs can relieve imidazole ketone erastin (IKE)-initiated ferroptosis-induced immune suppression and ultimately trigger the optimal therapeutic effect of the combined ferroptosis and ICB therapy is still unknown. Here, we report that TIPE2, a phospholipid transfer protein, regulated the ferroptosis susceptibility in MDSCs through reprogramming lipid peroxidation-related phosphatidylethanolamine (PE) and phosphatidylcholine (PC) species composition. TIPE2-deficient MDSCs resisted IKE-induced ferroptosis by up-regulating SLC7A11 and GPX4, and dissolved ferroptosis-induced immunosuppressive function by down-regulating lipid ROS whilst encouraging T cell proliferation and infiltration into tumor tissues to improve ferroptosis therapy. More importantly, TIPE2-deficient MDSCs achieved the full anti-tumor therapeutic potential of IKE-induced ferroptosis therapy and a PD-L1 blockade. These findings indicate that TIPE2 confers the ferroptosis sensitivity of MDSCs, and combining the targeting of the TIPE2 of MDSCs, ferroptosis therapy, and ICB is a novel therapeutic option for cancer treatment.
Differentiated Slip Casting: Producing Variable Thickness Ceramic Tiles with Functionally Graded Plaster Moulds
Authors: Baseta Efilena , Palma Marco , Heher Florian , Konegger Thomas , Kaftan Martin
Published: January 2025

This paper introduces a method that enhances the traditional slip casting technique’s potential to fabricate ceramic objects with variable thickness. The variability depends on the different filtration rates offered by plaster moulds of varying densities. Two sets of experiments are presented. They focused on identifying (1) the maximum workable density range of moulds made from plaster of Paris and (2) the range of thickness in the resulting ceramic casts. This was accomplished by creating four square flat moulds with different gypsum/water (G:W) ratios and their corresponding casts. Based on these findings, the second set of experiments focused on assembling graded plaster moulds with variable densities (G:W 1:3 to 2:1), resulting in ceramic tiles exhibiting a thickness gradient of 2 mm. These results suggest the possibility of producing double-curved ceramic objects (e.g., custom ceramic tiles or sanitaryware) with graded thickness, tailored to their desired structural and functional performance.
Volatolomics for Anticipated Diagnosis of Cancers with Chemoresistive Vapour Sensors: A Review
Authors: Sachan Abhishek , Castro Mickaël , Feller Jean-François
Published: January 2025

The anticipated diagnosis of cancers and other fatal diseases from the simple analysis of the volatiles emitted by the body (volatolome) is getting closer and closer from becoming reality. The promises of vapour sensor arrays are to provide a rapid, reliable, non-invasive and ready-to-use method for clinical applications by making an olfactive fingerprint characteristic of people’s health state, to increase their chance of early recovery. However, the different steps of this complex and ambitious process are still paved with difficulties needing innovative answers. The purpose of this review is to provide a statement of the blocs composing the diagnostic chain to identify the improvements still needed. Nanocomposite chemo-resistive transducers have unique prospects to enhance both the selectivity and sensitivity to volatile biomarkers. The variety of their formulations offers multiple possibilities to chemical functionalization and conductive architectures that should provide solutions to discriminations and stability issues. A focus will be made on the protocols for the collection of organic volatile compounds (VOC) from the body, the choice of vapour sensors assembled into an array (e-nose), in particular, chemo-resistive vapour sensors, their principle, fabrication and characteristics, and the way to extract pertinent features and analyse them with suitable algorithms that are able to find and produce a health diagnosis.
Scaphoid Fat Stripe Sign: Is It a Reliable Radiological Sign of Scaphoid Fracture in Children?
Authors: Manic Pavle , Schizas Stéphanie , Zambelli Pierre-Yves , Samara Eleftheria
Published: January 2025

Objectives: The scaphoid fat pad stripe (SFS) is a radiological sign first described in 1975 as a line of relative lucency lying parallel to the lateral border of the scaphoid, with slight convexity toward it, and it is optimally demonstrated on postero-anterior and oblique views with ulnar deviation of the carpus. The obliteration or displacement of this line is commonly present in acute fractures of the scaphoid, radial styloid process, and proximal first metacarpus. The aim of this observational study is to investigate the supportive value of the fat stripe sign (SFS) in the diagnosis of scaphoid fractures in the pediatric population. Methods: This is a monocentric, retrospective study of all patients referred to the Pediatric Traumatology Unit of a tertiary hospital from the Emergency Department with clinical suspicion of scaphoid fracture without visible fracture in the initial X-ray. Radiological reports for CT and MRIs were recorded, and the initial X-rays were blindly reviewed by a pediatric orthopedic fellowship-accredited surgeon for the presence of an abnormal scaphoid fat pad stripe sign and the presence of a fracture line in the initial X-rays. Results: The results of the blind interpretation of the initial X-rays for the fat stripe sign showed 86% sensitivity and 58% specificity, with the negative predictive value reaching 92%. Conclusions: The scaphoid fat stripe sign can be used as an adjacent in the diagnosis of an occult scaphoid fracture in children or adolescents. Its high negative predictive value, if confirmed in larger studies, can be an element used to exclude scaphoid fracture and consequently avoid unnecessary immobilizations and health costs.
Influence of Silica Fume (SF) Content on Passive Film Formation of Steel Reinforcement Inside Hardened Concrete
Authors: Amaral Marcella , Aguiar Oliveira Isaac , de Bem Diogo Henrique , Costa Réus Giovana , Macioski Gustavo , Miranda Farias Marcelo , Farias de Medeiros Marcelo Henrique
Published: January 2025

Corrosion is one of the causes of failure in reinforced concrete structures, and forming a passive film on the steel is essential for protection. Although several studies have looked at passive film formation in concrete pore solutions, few have considered its formation in hardened concrete and the influence of silica fume (SF) in the binder composition. This study aims to evaluate the influence of the SF content on passive film formation time in concrete. Periodic measurements assessed the electrical resistivity and corrosion current density of concrete samples containing 5%, 10%, 15%, and 20% SF. The alkalinity of the mixtures and the kinetics of the pozzolanic reaction were also monitored by XRD and titration tests. The control mixtures exhibited susceptibility to corrosion, regardless of the curing age evaluated. In contrast, the partial replacement of cement with SF accelerated the formation of the passive film on the steel surface, suggesting a delayed onset of corrosion due to modifications in the physical properties of the concrete. Also, the portlandite content and pH can predict passive film formation, with SF significantly accelerating this process.
Development of Gelatin/Zein Electrospun Nanofiber Films Containing Purple Sweet Potato Anthocyanin for Real-Time Freshness Monitoring of Aquatic Products
Authors: Wang Chenyu , Huang Aixia , Fang Jiaxuan , Li Shuangdie , Wu Siyu , Sun Di , Ma Qingbao , Yu Zhongjie , Liu Yu , Jiang Wei
Published: January 2025

In the present study, an electrospinning freshness monitoring film prepared by gelatin/zein loading with purple sweet potato anthocyanins (PSPA) was produced to track the freshness state of Penaeus vannamei. The electrospun nanofiber films with the gelatin and zein weight ratio of 1:0, 3:1, 2:1, and 1:1 were named GA, GZA 3:1, GZA 2:1, and GZA 1:1, respectively. The impacts of zein concentration on the electrospun nanofiber film properties were investigated. SEM results showed that a smooth surface was observed for the electrospun nanofiber films. As the zein content increased, the average diameter decreased. No new characteristic peaks were shown by FTIR and XRD, indicating the good compatibility between gelatin, zein, and PSPA. The incorporation of zein decreased the swelling ratio (from completely dissolved to 100.7%) and water solubility (from 100% to 30%) and increased the water contact angle (from 0° to 113.3°). The GA, GZA 3:1, GZA 2:1, and GZA 1:1 had apparent color changes to NH3 and demonstrated good stability and reversibility. Furthermore, the freshness states (fresh, sub-fresh, and spoiled) of Penaeus vannamei storage at 4 °C could be effectively distinguished by GZA 3:1 by showing different colors (from pink to grayish purple to blue). Consequently, GZA3:1 exhibited improved hydrophobicity and pH sensitivity and has great potential in real-time monitoring of aquatic product quality.
Combinational Circuits Testing Based on Hsiao Codes with Self-Dual Check Functions
Authors: Efanov Dmitry V. , Pogodina Tatiana S. , Aripov Nazirjan M. , Boltayev Sunnatillo T. , Azizov Asadulla R. , Ametova Elnara K. , Shakirova Feruza F.
Published: January 2025

This paper investigates the features of using modified Hamming codes, which are also known as Hsiao codes. Self-checking digital devices are proposed to be implemented with calculations testing using two diagnostic signs. These signs indicate that the functions (there are functions that describe check bits) belong to the class of self-dual Boolean functions and also belong to the codewords of Hsiao codes (these are codes with an odd column of weights). The authors have established that all check functions can be self-dual for a certain number of the Hsiao codes’ data symbols. Such codes can be used in the synthesis of concurrent error-detection circuits by two diagnostic signs. The paper describes the structure of an organization for a concurrent error-detection circuit based on Hsiao codes with self-dual check functions. Some experimental results are presented on the synthesis of self-checking devices using the proposed methodology. The controllability of the structure and the number of test combinations both increased. Hsiao codes can be effectively used with self-dual check functions in the synthesis of self-checking digital devices.
Exploring the Properties of Quantum Scars in a Toy Model
Authors: Sinha Sudip , Sinha Subhasis
Published: January 2025

We introduce the concept of ergodicity and explore its deviation caused by quantum scars in an isolated quantum system, employing a pedagogical approach based on a toy model. Quantum scars, originally identified as traces of classically unstable orbits in certain wavefunctions of chaotic systems, have recently regained interest for their role in non-ergodic dynamics, as they retain memory of their initial states. We elucidate these features of quantum scars within the same framework of this toy model. The integrable part of the model consists of two large spins, with a classical counterpart, which we combine with a random matrix to induce ergodic behavior. Scarred states can be selectively generated from the integrable spin Hamiltonian by protecting them from the ergodic states using a projector method. Deformed projectors mimic the ‘quantum leakage’ of scarred states, enabling tunable mixing with ergodic states and thereby controlling the degree of scarring. In this simple model, we investigate various properties of quantum scarring and shed light on different aspects of many-body quantum scars observed in more complex quantum systems. Notably, the underlying classicality can be revealed through the entanglement spectrum and the dynamics of ‘out-of-time-ordered correlators’.