bluuebunny/crossref_metadata_2025 huggingface dataset:
Increased HLA-G Expression in Term Placenta of Women with a History of Recurrent Miscarriage Despite Their Genetic Predisposition to Decreased HLA-G Levels
Authors: Craenmehr Moniek H. C. , Nederlof Iris , Cao Milo , Drabbels Jos J. M. , Spruyt-Gerritse Marijke J. , Anholts Jacqueline D. H. , Kapsenberg Hanneke M. , Stegehuis Janine A. , van der Keur Carin , Fasse Esther , Haasnoot Geert W. , van der Hoorn Marie-Louise P. , Claas Frans H. J. , Heidt Sebastiaan , Eikmans Michael
Published: February 2019

Human leukocyte antigen (HLA)-G is an immune modulating molecule that is present on fetal extravillous trophoblasts at the fetal-maternal interface. Single nucleotide polymorphisms (SNPs) in the 3 prime untranslated region (3′UTR) of the HLA-G gene can affect the level of HLA-G expression, which may be altered in women with recurrent miscarriages (RM). This case-control study included 23 women with a medical history of three or more consecutive miscarriages who delivered a child after uncomplicated pregnancy, and 46 controls with uncomplicated pregnancy. Genomic DNA was isolated to sequence the 3′UTR of HLA-G. Tissue from term placentas was processed to quantify the HLA-G protein and mRNA levels. The women with a history of RM had a lower frequency of the HLA-G 3′UTR 14-bp del/del genotype as compared to controls (Odds ratio (OR) 0.28; p = 0.039), which has previously been related to higher soluble HLA-G levels. Yet, HLA-G protein (OR 6.67; p = 0.006) and mRNA (OR 6.33; p = 0.010) expression was increased in term placentas of women with a history of RM as compared to controls. In conclusion, during a successful pregnancy, HLA-G expression is elevated in term placentas from women with a history of RM as compared to controls, despite a genetic predisposition that is associated with decreased HLA-G levels. These findings suggest that HLA-G upregulation could be a compensatory mechanism in the occurrence of RM to achieve an ongoing pregnancy.
Interaction of Halictine-Related Antimicrobial Peptides with Membrane Models
Authors: Pazderková Markéta , Maloň Petr , Zíma Vlastimil , Hofbauerová Kateřina , Kopecký Vladimír , Kočišová Eva , Pazderka Tomáš , Čeřovský Václav , Bednárová Lucie
Published: February 2019

We have investigated structural changes of peptides related to antimicrobial peptide Halictine-1 (HAL-1) induced by interaction with various membrane-mimicking models with the aim to identify a mechanism of the peptide mode of action and to find a correlation between changes of primary/secondary structure and biological activity. Modifications in the HAL-1 amino acid sequence at particular positions, causing an increase of amphipathicity (Arg/Lys exchange), restricted mobility (insertion of Pro) and consequent changes in antimicrobial and hemolytic activity, led to different behavior towards model membranes. Secondary structure changes induced by peptide-membrane interaction were studied by circular dichroism, infrared spectroscopy, and fluorescence spectroscopy. The experimental results were complemented by molecular dynamics calculations. An α-helical structure has been found to be necessary but not completely sufficient for the HAL-1 peptides antimicrobial action. The role of alternative conformations (such as β-sheet, PPII or 310-helix) also seems to be important. A mechanism of the peptide mode of action probably involves formation of peptide assemblies (possibly membrane pores), which disrupt bacterial membrane and, consequently, allow membrane penetration.
Are the Biological and Biomechanical Properties of Meniscal Scaffolds Reflected in Clinical Practice? A Systematic Review of the Literature
Authors: Ranmuthu Chanuka D. S. , Ranmuthu Charindu K. I. , Russell Jodie C. , Singhania Disha , Khan Wasim S.
Published: February 2019

The aim of this PRISMA review was to assess whether the CMI and Actifit scaffolds, when used in clinical practice, improve clinical outcomes and demonstrate the ideal biological and biomechanical properties of scaffolds: being chondroprotective, porous, resorbable, able to mature and promote regeneration of tissue. This was done by only including studies that assessed clinical outcome and used a scale to assess both integrity of the scaffold and its effects on articular cartilage via MRI. A search was performed on PubMed, EMBASE, Scopus and clinicaltrials.gov. 2457 articles were screened, from which eight studies were selected: four used Actifit, three used CMI and one compared the two. All studies reported significant improvement in at least one clinical outcome compared to baseline. Some studies suggested that the scaffolds appeared to show porosity, mature, resorb and/or have possible chondroprotective effects, as assessed by MRI. The evidence for clinical translation is limited by differences in study methodology and small sample sizes, but is promising in terms of improving clinical outcomes in the short to mid-term. Higher level evidence, with MRI and histological evaluation of the scaffold and articular cartilage, is now needed to further determine whether these scaffolds exhibit these useful properties.
Olanzapine Induced Dysmetabolic Changes Involving Tissue Chromium Mobilization in Female Rats
Authors: Yang Ching-Ping , Wang Ya-Yu , Lin Shih-Yi , Hong Yi-Jheng , Liao Keng-Ying , Hsieh Sheng-Kuo , Pan Ping-Ho , Chen Chun-Jung , Chen Wen-Ying
Published: February 2019

Atypical antipsychotics, such as olanzapine, are commonly prescribed to patients with schizophrenic symptoms and other psychiatric disorders. However, weight gain and metabolic disturbance cause adverse effects, impair patient compliance and limit clinical utility. Thus, a better understanding of treatment-acquired adverse effects and identification of targets for therapeutic intervention are believed to offer more clinical benefits for patients with schizophrenia. Beyond its nutritional effects, studies have indicated that supplementation of chromium brings about beneficial outcomes against numerous metabolic disorders. In this study, we investigated whether olanzapine-induced weight gain and metabolic disturbance involved chromium dynamic mobilization in a female Sprague-Dawley rat model, and whether a dietary supplement of chromium improved olanzapine-acquired adverse effects. Olanzapine medicated rats experienced weight gain and adiposity, as well as the development of hyperglycemia, hyperinsulinemia, insulin resistance, hyperlipidemia, and inflammation. The olanzapine-induced metabolic disturbance was accompanied by a decrease in hepatic Akt and AMP-activated Protein Kinase (AMPK) actions, as well as an increase in serum interleukin-6 (IL-6), along with tissue chromium depletion. A daily intake of chromium supplements increased tissue chromium levels and thermogenic uncoupling protein-1 (UCP-1) expression in white adipose tissues, as well as improved both post-olanzapine weight gain and metabolic disturbance. Our findings suggest that olanzapine medicated rats showed a disturbance of tissue chromium homeostasis by inducing tissue depletion and urinary excretion. This loss may be an alternative mechanism responsible for olanzapine-induced weight gain and metabolic disturbance.
Attention-Based Joint Entity Linking with Entity Embedding
Authors: Liu Chen , Li Feng , Sun Xian , Han Hongzhe
Published: February 2019

Entity linking (also called entity disambiguation) aims to map the mentions in a given document to their corresponding entities in a target knowledge base. In order to build a high-quality entity linking system, efforts are made in three parts: Encoding of the entity, encoding of the mention context, and modeling the coherence among mentions. For the encoding of entity, we use long short term memory (LSTM) and a convolutional neural network (CNN) to encode the entity context and entity description, respectively. Then, we design a function to combine all the different entity information aspects, in order to generate unified, dense entity embeddings. For the encoding of mention context, unlike standard attention mechanisms which can only capture important individual words, we introduce a novel, attention mechanism-based LSTM model, which can effectively capture the important text spans around a given mention with a conditional random field (CRF) layer. In addition, we take the coherence among mentions into consideration with a Forward-Backward Algorithm, which is less time-consuming than previous methods. Our experimental results show that our model obtains a competitive, or even better, performance than state-of-the-art models across different datasets.
CaACBIM: A Context-aware Access Control Model for BIM
Authors: Zheng Rongyue , Jiang Jianlin , Hao Xiaohan , Ren Wei , Xiong Feng , Zhu Tianqing
Published: February 2019

A building information model (BIM) is of upmost importance with a full life-time cycle in architecture engineering and construction industry. Smart construction relies on BIM to manipulate information flow, data flow, and management flow. Currently, BIM has been explored mainly for information construction and utilization, but there exist few works concerning information security, e.g., audits of critical models and exposure of sensitive models. Moreover, few BIM systems have been proposed to make use of new computing paradigms, such as mobile cloud computing, blockchain and Internet of Things. In this paper, we propose a Context-aware Access Control (CaAC) model for BIM systems on mobile cloud architectures. BIM data can be confidentially accessed according to contexts in a fine-grained manner. We describe functions of CaAC formally by illustrating location-aware access control and time-aware access control. CaAC model can outperform role-based access control for preventing BIM data leakage by distinguishing contexts. In addition, grouping algorithms are also presented for flexibility, in which basic model (user grouping based on user role permissions) and advanced model (user grouping based on user requests) are differentiated. Compared with the traditional role-based access control model, security and feasibility of CaAC are remarkably improved by distinguishing an identical role with multiple contexts. The average efficiency is improved by 2 n / ( 2 n − p − q ) , and time complexity is O ( n ) .
Investigations on the Grape Leafhopper Erasmoneura vulnerata in North-Eastern Italy
Authors: Duso Carlo , Moret Renzo , Manera Alessandro , Berto Dario , Fornasiero Diego , Marchegiani Gaia , Pozzebon Alberto
Published: February 2019

The leafhopper Erasmoneura vulnerata (Fitch) (Hemiptera: Cicadellidae) is native of Northern and Central America where it occurs on wild and cultivated grapes as well as on a number of secondary hosts. This species was recorded for the first time in Europe (Italy, Veneto region) in 2004. Since then it has spread over Northern Italy and Slovenia. Studies on the biology of E. vulnerata in America are limited and thus its phenology was investigated on Vitis labrusca L. and Vitis vinifera L. plants under field and semi-field conditions. These observations suggest that E. vulnerata can complete 2–3 generations per year. The development of E. vulnerata from first instar nymphs to adults was studied under controlled conditions (about 23 °C). Developmental times lasted from 16.1 days in July–August to 19.5 days in September, and this variability was probably due to grape cultivar and plant susceptibility. Data were consistent with the number of generations previously reported. Erasmoneura vulnerata was more abundant on vines close to overwintering sites than on those located 100–250 m from these sites and contiguous to commercial vineyards.
Automatically Monitoring, Controlling, and Reporting Status/Data for Multiple Product Life Test Stands
Authors: Fertell Richard , Ershad Hamed
Published: February 2019

This paper describes an industry laboratory implementation of local and remote automation monitoring and control of several product testing stands for status, safety, and an efficient use of resources, as well as the purpose and inter-relationship between the tests, product reliability estimations, customer applications, and possible solutions to test specification issues. Like an airport departure/arrival board, the summary statuses of several life stands are reported on a monitor using a mobile application interface. Detailed data analysis reports and emergency shutdown statuses are emailed to authorized-interested parties. The life stands include submersion tanks and flow rigs at different fluid temperatures (steady-state, slow cycling, thermal shock). To reduce equipment costs and lab space requirements, some life test stands are combined to share heating/cooling transfer fluids and pumping resources that are automatically controlled to direct different fluid temperature ranges through different flow paths. A heater/chilling system cycles between −80 °C and +140 °C with automated diverter valves to route −80 °C to +85 °C fluid through a set of products; when the fluid temperature is increased to > +85 °C, diverter valves route the > +85 °C fluid through a different set of products that are tested only between fluid temperatures of +85 °C to +140 °C.
Assessment of Renal Dysfunction Improves the Simplified Pulmonary Embolism Severity Index (sPESI) for Risk Stratification in Patients with Acute Pulmonary Embolism
Authors: Trimaille Antonin , Marchandot Benjamin , Girardey Mélanie , Muller Clotilde , Lim Han S. , Trinh Annie , Ohlmann Patrick , Moulin Bruno , Jesel Laurence , Morel Olivier
Published: February 2019

Background: Whereas the major strength of the simplified pulmonary embolism severity index (sPESI) lies in ruling out an adverse outcome in patients with sPESI of 0, the accuracy of sPESI ≥ 1 in risk assessment remains questionable. In acute pulmonary embolism (APE), the estimated glomerular filtration rate (eGFR) can be viewed as an integrate marker reflecting not only previous chronic kidney disease (CKD) damage but also comorbid conditions and hemodynamic disturbances associated with APE. We sought to determine whether renal dysfunction assessment by eGFR improves the sPESI score risk stratification in patients with APE. Methods: 678 consecutive patients with APE were prospectively enrolled. Renal dysfunction (RD) at diagnosis of APE was defined by eGFR < 60 mL/min/1.73 m2 and acute kidney injury (AKI) by elevation of creatinine level >25% during in-hospital stay. Results: RD was observed in 26.9% of the cohort. AKI occurred in 18.8%. A stepwise increase in 30-day mortality, cardiovascular mortality and overall mortality was evident with declining renal function. Multivariate analysis identified RD and CRP (C-reactive protein) level but not sPESI score as independent predictors of 30-day mortality. AKI, 30-day mortality, overall mortality, and cardiovascular mortality were at their highest level in patients with eGFR < 60 mL/min/1.73 m2 and sPESI ≥1. Conclusion: in patients with APE, the addition of RD to the sPESI score identifies a specific subset of patients at very high mortality.
Changing Spectrum of Opportunistic Illnesses among HIV-Infected Taiwanese Patients in Response to a 10-Year National Anti-TB Programme
Authors: Lee Chun-Yuan , Wu Pei-Hua , Lu Po-Liang , Tsai Hung-Chin
Published: February 2019

The current trends and spectrum of acquired immunodeficiency syndrome (AIDS)-related opportunistic illnesses (AOIs) among newly diagnosed human immunodeficiency virus (HIV)-infected patients after the implementation of the 2006–2015 national anti-tuberculosis (TB) programmes in Taiwan remain unknown. We retrospectively reviewed 1757 patients at two centres in southern Taiwan between 2001 and 2015. Based on the anti-TB programme, patients were classified into periods 1 (2001–2005), 2 (2006–2010), and 3 (2011–2015). We further analysed factors associated with Mycobacterium tuberculosis (MTB) at presentation and during follow-up. The overall AOI incidence rate (23.6%) remained unchanged across the periods, with 81.4% of AOIs occurring at presentation. Pneumocystis jirovecii pneumonia was the leading AOI across the periods. MTB declined significantly from period 1 to period 3 (39.3% vs. 9.3%). Age and CD4+ cell count <200 cells/µL (vs. ≥501) were the risk factors associated with MTB at presentation, whereas period 2/3 (vs. period 1) was the protective factor. Intravenous drug use (vs. homosexual contact) was the risk factor associated with MTB during follow-up, and period 3 (vs. period 1) was the protective factor. AOI statistics in Taiwan must be closely monitored for fluctuations. Although MTB decreased substantially after implementation of the anti-TB programmes, additional efforts to reduce MTB are required.
Incremental Predictive Value of Longitudinal Axis Strain and Late Gadolinium Enhancement Using Standard CMR Imaging in Patients with Aortic Stenosis
Authors: Agoston-Coldea Lucia , Bheecarry Kunal , Cionca Carmen , Petra Cristian , Strimbu Lelia , Ober Camelia , Lupu Silvia , Fodor Daniela , Mocan Teodora
Published: February 2019

To analyse the predictive ability and incremental value of left ventricular longitudinal axis strain (LAS) and late gadolinium enhancement (LGE) using standard cardiovascular magnetic resonance (CMR) imaging for the diagnosis and prognosis of severe aortic stenosis (AS) in patients with an indication for aortic valve replacement. We conducted a prospective study on 52 patients with severe AS and 52 volunteers. The evaluation protocol included standard biochemistry tests, novel biomarkers of myocardial fibrosis, 12-lead electrocardiograms and 24-hour Holter, the 6-minute walk test and extensive echocardiographic and CMR imaging studies. Outcomes were defined as the composite of major cardiovascular events (MACEs). Among AS patients, most (n = 17, 77.2%) of those who exhibited LGE at CMR imaging had MACEs during follow-up. Kaplan–Meier curves for event-free survival showed a significantly higher rate of MACEs in patients with LGE (p < 0.01) and decreased LAS (p < 0.001). In Cox regression analysis, only reduced LAS (hazard ratio 1.33, 95% CI (1.01 to 1.74), p < 0.01) and the presence of LGE (hazard ratio 11.3, 95% CI (1.82 to 70.0), p < 0.01) were independent predictors for MACEs. The predictive value increased if both LGE and reduced LAS were added to left ventricular ejection fraction (LVEF). None of the biomarkers of increased collagen turnover exhibited any predictive value for MACEs. LAS by CMR is an independent predictor of outcomes in patients with AS and provides incremental value beyond the assessment of LVEF and the presence of LGE.
The Forgotten Skeletogenic Condensations: A Comparison of Early Skeletal Development Amongst Vertebrates
Authors: Giffin Jennifer L. , Gaitor Danielle , Franz-Odendaal Tamara A.
Published: February 2019

The development of a skeletogenic condensation is perhaps the most critical yet considerably overlooked stage of skeletogenesis. Described in this comprehensive review are the mechanisms that facilitate skeletogenic condensation formation, growth, and maintenance to allow for overt differentiation into a skeletal element. This review discusses the current knowledge of gene regulation and characterization of skeletogenic condensations in the chicken, mouse, zebrafish, and other developmental models. We limited our scope to condensations that give rise to the bones and cartilages of the vertebrate skeleton, with a particular focus on craniofacial and limb bud regions. While many of the skeletogenic processes are similar among vertebrate lineages, differences are apparent in the site and timing of the initial epithelial–mesenchymal interactions as well as in whether the condensation has an osteogenic or chondrogenic fate, both within and among species. Further comparative studies are needed to clarify and broaden the existing knowledge of this intricate phenomenon.
KNSwing—On the Mooring Loads of a Ship-Like Wave Energy Converter
Authors: Nielsen Kim , Thomsen Jonas Bjerg
Published: February 2019

The critical function of keeping a floating Wave Energy Converter in position is done by a mooring system. Several WECs have been lost due to failed moorings, indicating that extreme loads, reliability and durability are very important aspects. An understanding of the interaction between the WEC’s motion in large waves and the maximum mooring loads can be gained by investigating the system at model scale supported by numerical models. This paper describes the testing of a novel attenuator WEC design called KNSwing. It is shaped like a ship facing the waves with its bow, which results in low mooring loads and small motions in most wave conditions when the structure is longer than the waves. The concept is tested using an experimental model at scale 1:80 in regular and irregular waves, moored using rubber bands to simulate synthetic moorings. The experimental results are compared to numerical simulations done using the OrcaFlex software. The experimental results show that the WEC and the mooring system survives well, even under extreme and breaking waves. The numerical model coefficient concerning the nonlinear drag term for the surge motion is validated using decay tests. The numerical results compare well to the experiments and, thereby, the numerical model can be further used to optimize the mooring system.
Predicting Land Use Changes in Philadelphia Following Green Infrastructure Policies
Authors: Shade Charlotte , Kremer Peleg
Published: February 2019

Urbanization is a rapid global trend, leading to consequences such as urban heat islands and local flooding. Imminent climate change is predicted to intensify these consequences, forcing cities to rethink common infrastructure practices. One popular method of adaptation is green infrastructure implementation, which has been found to reduce local temperatures and alleviate excess runoff when installed effectively. As cities continue to change and adapt, land use/landcover modeling becomes an important tool for city officials in planning future land usage. This study uses a combination of cellular automata, machine learning, and Markov chain analysis to predict high resolution land use/landcover changes in Philadelphia, PA, USA for the year 2036. The 2036 landcover model assumes full implementation of Philadelphia’s green infrastructure program and past temporal trends of urbanization. The methodology used to create the 2036 model was validated by creating an intermediate prediction of a 2015 landcover that was then compared to an existing 2015 landcover. The accuracy of the validation was determined using Kappa statistics and disagreement scores. The 2036 model successfully met Philadelphia’s green infrastructure goals. A variety of landscape metrics demonstrated an overall decrease in fragmentation throughout the landscape due to increases in urban landcover.
Judging Values and Participation in Mental Capacity Law
Authors: Kong Camillia , Coggon John , Dunn Michael , Cooper Penny
Published: February 2019

Judges face a challenging task in determining the weight that ought to be accorded to the person (P)’s values and testimony in judicial deliberation about her capacity and best interests under the Mental Capacity Act 2005 (MCA). With little consensus emerging in judicial practice, incommensurable values drawn from divergent sources often collide in such cases. This paper outlines strict and flexible interpretations of the MCA’s values-based approach to making decisions about capacity and best interests, highlighting the problematic implications for the normative status of P’s values and the participatory role of P in judicial deliberations. The strict interpretation draws a false separation between ascertaining P’s values and the intrinsic value of enabling P’s participation in court proceedings; meanwhile, the flexible interpretation permits judicial discretion to draw on values which may legitimately override the expressed values of P. Whether in the ambiguous form of internal and/or extra-legal judicial values, these value sources demand further scrutiny, particularly regarding their intersection with the values held by P. We offer provisional normative guidelines, which set constraints on the appeal to extra-legal values in judicial deliberation and outline further research pathways to improve the justification around judicial decisions regarding P’s participation.
Microfluidic Reactors for Carbon Fixation under Ambient-Pressure Alkaline-Hydrothermal-Vent Conditions
Authors: Sojo Victor , Ohno Aya , McGlynn Shawn E. , Yamada Yoichi M.A. , Nakamura Ryuhei
Published: February 2019

The alkaline-hydrothermal-vent theory for the origin of life predicts the spontaneous reduction of CO2, dissolved in acidic ocean waters, with H2 from the alkaline vent effluent. This reaction would be catalyzed by Fe(Ni)S clusters precipitated at the interface, which effectively separate the two fluids into an electrochemical cell. Using microfluidic reactors, we set out to test this concept. We produced thin, long Fe(Ni)S precipitates of less than 10 µm thickness. Mixing simplified analogs of the acidic-ocean and alkaline-vent fluids, we then tested for the reduction of CO2. We were unable to detect reduced carbon products under a number of conditions. As all of our reactions were performed at atmospheric pressure, the lack of reduced carbon products may simply be attributable to the low concentration of hydrogen in our system, suggesting that high-pressure reactors may be a necessity.
CuPc: Effects of its Doping and a Study of Its Organic-Semiconducting Properties for Application in Flexible Devices
Authors: Leyva Esqueda Mariel , Sánchez Vergara María Elena , Álvarez Bada José Ramón , Salcedo Roberto
Published: February 2019

This study refers to the doping of organic semiconductors by a simple reaction between copper phthalocyanine and tetrathiafulvalene or tetracyanoquinodimethane. The semiconductor films of copper phthalocyanine, doped with tetrathiafulvalene donor (CuPc-TTF) and tetracyanoquinodimethane acceptor (CuPc-TCNQ) on different substrates, were prepared by vacuum evaporation. The structure and morphology of the semiconductor films were studied with infrared (IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM). The absorption spectra for CuPc-TTF, recorded in the 200–900 nm UV–vis region for the deposited films, showed two peaks: a high energy peak, around 613 nm, and a second one, around 695 nm, with both peaks corresponding to the Q-band transition of the CuPcs. From the spectra, it can also be seen that CuPc-TTF has a B-band at around 330 nm and has a bandgap of approximately 1.4 eV. The B-band in the CuPc-TCNQ spectrum is quite similar to that of CuPc-TTF; on the other hand, CuPc-TCNQ does not include a Q-band in its spectrum and its bandgap value is of approximately 1.6 eV. The experimental optical bandgaps were compared to the ones calculated through density functional theory (DFT). In order to prove the effect of dopants in the phthalocyanine semiconductor, simple devices were manufactured and their electric behaviors were evaluated. Devices constituted by the donor-acceptor active layer and by the hollow, electronic-transport selective layers, were deposited on rigid and flexible indium tin oxide (ITO) substrates by the vacuum sublimation method. The current–voltage characteristics of the investigated structures, measured in darkness and under illumination, show current density values of around 10 A/cm2 for the structure based on a mixed-PET layer and values of 3 A/cm2 for the stacked-glass layered structure. The electrical properties of the devices, such as carrier mobility (μ) were obtained from the J–V characteristics. The mobility values of the devices on glass were between 1.59 × 109 and 3.94 × 1010 cm2/(V·s), whereas the values of the devices on PET were between 1.84 × 109 and 4.51 × 109 cm2/(V·s). The different behaviors of the rigid and flexible devices is mainly due to the effect of the substrate.
Performance of Nano-Silica Modified Self-Compacting Glass Mortar at Normal and Elevated Temperatures
Authors: Natarajan Sakthieswaran , Udayabanu Muthuraman , Ponnan Suresh , Murugan Sophia
Published: February 2019

This research aims to combine the effects of nanosilica and glass powder on the properties of self-compacting mortar at normal and at higher temperatures. The fine aggregate was replaced by waste glass powder at various percentage levels of 10%, 20%, 30%, 40% and 50%. The mechanical properties of self-compacting glass mortar (SGCM) were studied at elevated temperatures of 200, 400, 600 and 800 °C. Furthermore the effect of sudden and gradual cooling technique on the residual strength of glass mortar was also investigated In order to enhance the behavior of SCGM the nanosilica of 3% by weight of cement was added. From the results it was obtained that the glass powder replacement effectively contributed towards the thermal performance while the addition of nanosilica enhanced the mechanical performance. The enhanced physical properties were obtained mainly at the glass transition temperature thus showing the active participation of glass powders during high temperatures. Moreover the gradually cooled specimens exhibited improved strength characteristics than the suddenly cooled specimens.
Metakaolinite Phosphate Cementitious Matrix: Inorganic Polymer Obtained by Acidic Activation
Authors: Katsiki Antigoni , Hertel Tobias , Tysmans Tine , Pontikes Yiannis , Rahier Hubert
Published: February 2019

This work aims to study an aluminosilicate phosphate cementitious matrix. The cementitious matrix was studied on paste samples. The synthesis of metakaolinite phosphate cement (MKPC) was investigated using calorimetric techniques. A systematic study was performed by emphasizing a broad range of Al/P molar ratios, covering the different behavior of the material to the extremes, as well as the optimum composition. X-ray diffraction and scanning electron microscopy revealed that the final structure was mainly an amorphous network, albeit with some non-reacted phases. The compressive strength was studied on mortars using a cement/sand ratio of 1:3. MKPC specimens with Al/P ratios close to 1/1 showed optimal behavior. MKPCs with Al/P ratios above 1/1 were characterized by high porosity and low strength, whereas MKPCs with Al/P < 1 contained an excess of phosphates. The influence of the Al/P molar ratio on compressive strength was also studied, reaching a maximum of 68 MPa for the optimum composition. Based on the results, MKPC may be a promising candidate for construction purposes.
Modeling and Optimization of Fractal Dimension in Wire Electrical Discharge Machining of EN 31 Steel Using the ANN-GA Approach
Authors: Mukhopadhyay Arkadeb , Barman Tapan Kumar , Sahoo Prasanta , Davim J. Paulo
Published: February 2019

To achieve enhanced surface characteristics in wire electrical discharge machining (WEDM), the present work reports the use of an artificial neural network (ANN) combined with a genetic algorithm (GA) for the correlation and optimization of WEDM process parameters. The parameters considered are the discharge current, voltage, pulse-on time, and pulse-off time, while the response is fractal dimension. The usefulness of fractal dimension to characterize a machined surface lies in the fact that it is independent of the resolution of the instrument or length scales. Experiments were carried out based on a rotatable central composite design. A feed-forward ANN architecture trained using the Levenberg-Marquardt (L-M) back-propagation algorithm has been used to model the complex relationship between WEDM process parameters and fractal dimension. After several trials, 4-3-3-1 neural network architecture has been found to predict the fractal dimension with reasonable accuracy, having an overall R-value of 0.97. Furthermore, the genetic algorithm (GA) has been used to predict the optimal combination of machining parameters to achieve a higher fractal dimension. The predicted optimal condition is seen to be in close agreement with experimental results. Scanning electron micrography of the machined surface reveals that the combined ANN-GA method can significantly improve the surface texture produced from WEDM by reducing the formation of re-solidified globules.
Modification of Residual Stresses in Laser Additive Manufactured AlSi10Mg Specimens Using an Ultrasonic Peening Technique
Authors: Xing Xiaodong , Duan Xiaoming , Sun Xiaojing , Gong Haijun , Wang Liquan , Jiang Fengchun
Published: February 2019

Ultrasonic peening treatment (UPT) has been proved to be an effective way of improving residual stresses distribution in weld structures. Thus, it shows a great potential in stress modification for metal parts fabricated by additive manufacturing technology. In this paper, an investigation into the ultrasonic treatment process of AlSi10Mg specimens fabricated by selective laser melting (SLM) process was conducted by means of experimental and numerical simulation. The specimens were prepared using a SLM machine, and UPT on their top surface was carried out. The residual stresses were measured with an X-ray stress diffraction device before and after UPT. Meanwhile, a finite element simulation method for analyzing the influence of UPT on the residual stress field of specimens was proposed and validated by experiments. Firstly, the thermal mechanical coupling numerical simulation of the SLM process of the specimen was carried out in order to obtain the residual stress distribution in the as-fabricated specimen. Then, the transient dynamic finite element simulation model of the UPT process of the specimen was established, and the UPT effect analysis was implemented. In the UPT simulation, the residual stress was applied as a pre-stress on the specimen, and the specimen’s material mechanical property was described by the Johnson–Cook model, whose parameters were determined by Split Hopkinson Pressure Bar (SHPB) experiment. The residual stress distribution before and after UPT predicted by the finite element model agree well with the measurement results. This paper concludes with a discussion of the effects of ultrasonic peening time, as well as the frequency and amplitude of the peening needle on residual stress.
A Theory of Elastic/Plastic Plane Strain Pure Bending of FGM Sheets at Large Strain
Authors: Alexandrov Sergey , Wang Yun-Che , Lang Lihui
Published: February 2019

An efficient analytical/numerical method has been developed and programmed to predict the distribution of residual stresses and springback in plane strain pure bending of functionally graded sheets at large strain, followed by unloading. The solution is facilitated by using a Lagrangian coordinate system. The study is concentrated on a power law through thickness distribution of material properties. However, the general method can be used in conjunction with any other through thickness distributions assuming that plastic yielding initiates at one of the surfaces of the sheet. Effects of material properties on the distribution of residual stresses are investigated.
Hydration and Microstructure of Cement Pastes with Calcined Hwangtoh Clay
Authors: Lin Run-Sheng , Wang Xiao-Yong , Lee Han-Seung , Cho Hyeong-Kyu
Published: February 2019

Calcined Hwangtoh (HT) clay is a very promising supplementary cementitious material (SCM). In this work, the development of the mechanical properties and microstructures of HT-blended cement paste was studied after substituting the binder with HT powder calcined at 800 °C. The water-to-binder (w/b) ratios of the paste used were 0.2 and 0.5, and the quantities of HT powder added to the mixture were 0, 10, and 20%. The compressive strength test indicates that the addition of the HT powder increases the compressive strength of the paste after seven days of curing, and the highest compressive strength is obtained with the 10% HT substitution, regardless of whether the w/b ratio is 0.5 or 0.2. X-ray fluorescence (XRF), X-ray diffraction (XRD), inductively coupled plasma mass spectrometry (ICP-MS), isothermal calorimetry, thermogravimetric analysis (TGA), and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) analysis show that the HT powder not only has a physical effect (i.e., nucleation effect and dilution effect) on cement hydration but also has a chemical effect (i.e., chemical reaction of HT). The results of scanning electron microscopy (SEM) and mercury intrusion porosimetry (MIP) analysis show that the paste has more ettringite during the early stage, and the microstructure is refined after the addition of the HT powder. In addition, the relationships between chemically bound water, hydration heat, and compressive strength are presented.
New Refinement of the Operator Kantorovich Inequality
Authors: Moradi Hamid Reza , Furuichi Shigeru , Heydarbeygi Zahra
Published: February 2019

We focus on the improvement of operator Kantorovich type inequalities. Among the consequences, we improve the main result of the paper [H.R. Moradi, I.H. Gümüş, Z. Heydarbeygi, A glimpse at the operator Kantorovich inequality, Linear Multilinear Algebra, doi:10.1080/03081087.2018.1441799].
Antioxidant and Neuroprotective Potential of the Brown Seaweed Bifurcaria bifurcata in an in vitro Parkinson’s Disease Model
Authors: Silva Joana , Alves Celso , Freitas Rafaela , Martins Alice , Pinteus Susete , Ribeiro Joana , Gaspar Helena , Alfonso Amparo , Pedrosa Rui
Published: February 2019

Bifurcaria bifurcata is a marine brown seaweed mainly found on the Atlantic coast. Herein, we report the antioxidant and neuroprotective activities of seven fractions (F1–F7) obtained by normal phase chromatography from the B. bifurcata dichloromethane extract, as well as of its two major isolated diterpenes. Total phenolic content of fractions was determined by the Folin–Ciocalteu method, while antioxidant activity was evaluated by the DPPH, ORAC, and FRAP assays. Neuroprotective effects were evaluated in a neurotoxic model induced by 6-hydroxydopamine (6-OHDA) in a human neuroblastoma cell line (SH-SY5Y), while the mechanisms associated to neuroprotection were investigated by the determination of mitochondrial membrane potential, H2O2 production, Caspase-3 activity, and by observation of DNA fragmentation. Fractions F4 and F5 exhibited the best neuroprotective and antioxidant activities, respectively. F4 fraction prevented changes in mitochondrial potential, and induced a reduction of H2O2 levels production and an increase in cell viability, suggesting that it may contain multi-target compounds acting on different pathways. Hence, this fraction was subjected to purification steps, affording the known diterpenes eleganolone and eleganonal. Both compounds exhibited antioxidant potential, being interesting candidates for further neuroprotective studies.
Molecular Networking-Based Analysis of Cytotoxic Saponins from Sea Cucumber Holothuria atra
Authors: Grauso Laura , Yegdaneh Afsaneh , Sharifi Mohsen , Mangoni Alfonso , Zolfaghari Behzad , Lanzotti Virginia
Published: February 2019

The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy. The saponin composition of Holothuria atra turned out to be more complex than previously reported. The most abundant saponins in the extract (1–4) were isolated and characterized by 1D- and 2D-NMR experiments. Compound 1 was identified as a new triterpene glycoside saponin, holothurin A5. The side chain of the new saponin 1, unprecedented among triterpene glycosides, is characterized by an electrophilic enone function, which can undergo slow water or methanol addition under neutral conditions. The cytotoxic activity of compounds 1–4, evaluated on the human cervix carcinoma HeLa cell line, was remarkable, with IC50 values ranging from 1.2 to 2.5 µg/mL.
Four New Isocoumarins and a New Natural Tryptamine with Antifungal Activities from a Mangrove Endophytic Fungus Botryosphaeria ramosa L29
Authors: Wu Zhihui , Chen Jiaqing , Zhang Xiaolin , Chen Zelin , Li Tong , She Zhigang , Ding Weijia , Li Chunyuan
Published: February 2019

Four new isocoumarin derivatives, botryospyrones A (1), B (2), C (3), and D (4), and a new natural tryptamine, (3aS, 8aS)-1-acetyl-1, 2, 3, 3a, 8, 8a-hexahydropyrrolo [2,3b] indol-3a-ol (5), were isolated from a marine mangrove endophytic fungus Botryosphaeria ramosa L29, obtained from the leaf of Myoporum bontioides. Their structures were elucidated using spectroscopic analysis. The absolute configurations of compounds 3, 4, and 5 were determined by comparison of their circular dichroism (CD) spectra with the calculated data. The inhibitory activities of compound 1 on Fusarium oxysporum, of compounds 2 and 3 on F. oxysporum and Fusarium graminearum, and of compound 5 on F. oxysporum, Penicillium italicum, and F. graminearum were higher than those of triadimefon, widely used as an agricultural fungicide. Compound 5 was produced after using the strategy we called “using inhibitory stress from components of the host” (UISCH), wherein (2R, 3R)-3, 5, 7-trihydroxyflavanone 3-acetate, a component of M. bontioides with weak growth inhibitory activity towards B. ramosa L29, was introduced into the culture medium.
Novel Antimicrobial Indolepyrazines A and B from the Marine-Associated Acinetobacter sp. ZZ1275
Authors: Anjum Komal , Kaleem Sidra , Yi Wenwen , Zheng Guowan , Lian Xiaoyuan , Zhang Zhizhen
Published: February 2019

Two new alkaloids indolepyrazines A (1) and B (2) were isolated from the marine-derived Acinetobacter sp. ZZ1275. Their structures were elucidated through extensive nuclear magnetic resonance (NMR) spectroscopic analyses, high resolution electrospray ionization mass spectroscopy (HRESIMS) data, and electronic circular dichroism (ECD) calculation. Indolepyrazine A represents the first example of alkaloids with an indole-pyrazine-oxindole skeleton. Both 1 and 2 showed antimicrobial activities against methicillin-resistant Staphylococcus aureus, Escherichia coli, and Candida albicans with minimum inhibitory concentration (MIC) values of 12 μg/mL, 8–10 μg/mL, and 12–14 μg/mL, respectively.
Jellyfish-Associated Microbiome in the Marine Environment: Exploring Its Biotechnological Potential
Authors: Tinta Tinkara , Kogovšek Tjaša , Klun Katja , Malej Alenka , Herndl Gerhard J. , Turk Valentina
Published: February 2019

Despite accumulating evidence of the importance of the jellyfish-associated microbiome to jellyfish, its potential relevance to blue biotechnology has only recently been recognized. In this review, we emphasize the biotechnological potential of host–microorganism systems and focus on gelatinous zooplankton as a host for the microbiome with biotechnological potential. The basic characteristics of jellyfish-associated microbial communities, the mechanisms underlying the jellyfish-microbe relationship, and the role/function of the jellyfish-associated microbiome and its biotechnological potential are reviewed. It appears that the jellyfish-associated microbiome is discrete from the microbial community in the ambient seawater, exhibiting a certain degree of specialization with some preferences for specific jellyfish taxa and for specific jellyfish populations, life stages, and body parts. In addition, different sampling approaches and methodologies to study the phylogenetic diversity of the jellyfish-associated microbiome are described and discussed. Finally, some general conclusions are drawn from the existing literature and future research directions are highlighted on the jellyfish-associated microbiome.
Diphlorethohydroxycarmalol Attenuates Fine Particulate Matter-Induced Subcellular Skin Dysfunction
Authors: Zhen Ao Xuan , Piao Mei Jing , Hyun Yu Jae , Kang Kyoung Ah , Madushan Fernando Pincha Devage Sameera Madushan , Cho Suk Ju , Ahn Mee Jung , Hyun Jin Won
Published: February 2019

The skin, the largest organ in humans, is exposed to major sources of outdoor air pollution, such as fine particulate matter with a diameter ≤ 2.5 µm (PM2.5). Diphlorethohydroxycarmalol (DPHC), a marine-based compound, possesses multiple activities including antioxidant effect. In the present study, we evaluated the protective effect of DPHC on PM2.5-induced skin cell damage and elucidated the underlying mechanisms in vitro and in vivo. The results showed that DPHC blocked PM2.5-induced reactive oxygen species generation in human keratinocytes. In addition, DPHC protected cells against PM2.5-induced DNA damage, endoplasmic reticulum stress, and autophagy. HR-1 hairless mice exposed to PM2.5 showed lipid peroxidation, protein carbonylation, and increased epidermal height, which were inhibited by DPHC. Moreover, PM2.5 induced apoptosis and mitogen-activated protein kinase (MAPK) protein expression; however, these changes were attenuated by DPHC. MAPK inhibitors were used to elucidate the molecular mechanisms underlying these actions, and the results demonstrated that MAPK signaling pathway may play a key role in PM2.5-induced skin damage.
Promising Recent Strategies with Potential Clinical Translational Value to Combat Antibacterial Resistant Surge
Authors: Karmakar Partha , Gaitonde Vishwanath
Published: February 2019

Multiple drug resistance (MDR) for the treatment of bacterial infection has been a significant challenge since the beginning of the 21st century. Many of the small molecule-based antibiotic treatments have failed on numerous occasions due to a surge in MDR, which has claimed millions of lives worldwide. Small particles (SPs) consisting of metal, polymer or carbon nanoparticles (NPs) of different sizes, shapes and forms have shown considerable antibacterial effect over the past two decades. Unlike the classical small-molecule antibiotics, the small particles are less exposed so far to the bacteria to trigger a resistance mechanism, and hence have higher chances of fighting the challenge of the MDR process. Until recently, there has been limited progress of clinical treatments using NPs, despite ample reports of in vitro antibacterial efficacy. In this review, we discuss some recent and unconventional strategies that have explored the antibacterial efficacy of these small particles, alone and in combination with classical small molecules in vivo, and demonstrate possibilities that are favorable for clinical translations in near future.
Membrane Fouling Due to Protein—Polysaccharide Mixtures in Dead-End Ultrafiltration; the Effect of Permeation Flux on Fouling Resistance
Authors: Sioutopoulos Dimitrios , Karabelas Anastasios , Mappas Vasileios
Published: February 2019

Significant gaps exist in our knowledge of ultrafiltration (UF) membrane fouling, due to mixtures of poly-saccharides and proteins, despite a fair amount of related research. To get new insights into fouling layer characteristics, experiments were performed under constant-flux, within the range of practical interest (15–90 L/m2h), with typical polysaccharides (sodium alginate, SA), proteins (bovine serum albumin, BSA) as well as their mixtures in various proportions (1:3, 1:1, 3:1), and total organic matter concentration of 30 mg/L. The feed-water salinity and calcium ion concentration were 2000 mg/L NaCl and 2 mM, respectively. The temporal evolution of such fouling layers on flat-sheet membranes was monitored by recording the trans-membrane pressure variation. The results show that the specific fouling resistance α is strongly affected by flux, and the fouling propensity of polysaccharide-protein mixtures is significantly enhanced compared to single foulants, i.e., when BSA and SA are alone. The fouling layers are compressible and their resistance α tends to increase with the mass ratio of alginate in the mixture, particularly at high fluxes. To quantify these effects, correlations are presented of the initial fouling resistance αi with permeate flux J and of the evolution of α. R&D priorities are suggested on this topic of mixed foulants.