bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic Variability of Mass-Selected and Wild Populations of Yellow Drum (Nibea albiflora) Revealed Using Microsatellites
Authors: Yu Qin , Liu Simiao , Zhu Qihui , Chen Ruiyi , Hu Weihua , Xu Dongdong
Published: January 2024

In this study, twelve polymorphic microsatellite loci were screened to evaluate the genetic diversity of five yellow drum (Nibea albiflora) populations in the Zhoushan Sea region of the East China Sea, including one wild population (WP), one artificially propagated population (common population, CP), and three breeding populations (parent population, F4; all-female population, AF; and neo-male population, NeoG). The results of genetic diversity analyses showed that all five yellow drum populations had relatively high genetic diversity, with the highest in WP and the lowest in NeoG. Genetic structure analyses showed that the level of genetic differentiation among populations was low, with that between CP and F4 being the largest, whereas that between CP and WP was the smallest. Mutation–drift equilibrium analysis showed that the five populations likely did not experience a recent bottleneck. Our results suggest the CP population was the most suitable for large-scale release for stock enhancement, and precautionary measures shall be taken for the AF population before it is used for cage culture to avoid potential genetic concerns of the wild population. Nevertheless, further genetic diversity monitoring is needed to evaluate genetic effects and avoid the negative impact of excessive genetic differentiation between breeding and wild populations.
Bio-Mapping Salmonella and Campylobacter Loads in Three Commercial Broiler Processing Facilities in the United States to Identify Strategic Intervention Points
Authors: Chavez-Velado Daniela R. , Vargas David A. , Sanchez-Plata Marcos X.
Published: January 2024

The poultry industry in the United States is one of the largest in the world. Poultry consumption has significantly increase since the COVID-19 pandemic and is predicted to increase over 16% between 2021 and 2030. Two of the most significant causes of hospitalizations and death in the United States are highly related to poultry consumption. The FSIS regulates poultry processing, enforcing microbial performance standards based on Salmonella and Campylobacter prevalence in poultry processing establishments. This prevalence approach by itself is not a good indicator of food safety. More studies have shown that it is important to evaluate quantification along with prevalence, but there is not much information about poultry mapping using quantification and prevalence. In this study, enumeration and prevalence of Salmonella and Campylobacter were evaluated throughout the process at three different plants in the United States. Important locations were selected in this study to evaluate the effect of differences interventions. Even though there were high differences between the prevalences in the processes, some of the counts were not significantly different, and they were effective in maintaining pathogens at safe levels. Some of the results showed that the intervention and/or process were not well controlled, and they were not effective in controlling pathogens. This study shows that every plant environment is different, and every plant should be encouraged to implement a bio-mapping study. Quantification of pathogens leads to appropriate risk assessment, where physical and chemical interventions can be aimed at specific processing points with higher pathogen concentrations using different concentrations of overall process improvement.
Red Onion Peel Powder as a Functional Ingredient for Manufacturing Ricotta Cheese
Authors: Lipșa Florin Daniel , Stoica Florina , Rațu Roxana Nicoleta , Veleșcu Ionuț Dumitru , Cârlescu Petru Marian , Motrescu Iuliana , Usturoi Marius Giorgi , Râpeanu Gabriela
Published: January 2024

Onion (Allium cepa L.) is a vegetable widely cultivated and consumed due to its rich content in bioactive compounds. Red onion peel (ROP) powder, which is a by-product derived from the onion industry, has been attracting significant interest as a potential functional ingredient for improving the overall quality of foods. The present study explores the potential of ROP powder as a functional ingredient to improve the quality and nutritional value of whey cheese. Despite being frequently viewed as a food processing waste byproduct, ROP is a rich source of bioactive substances, including antioxidants, flavonoids, and dietary fiber, having antioxidant and antibacterial effects. The ROP extract exhibited high amounts of total polyphenols (119.69 ± 2.71 mg GAE/g dw) and antioxidant activity (82.35 ± 1.05%). Different quantities (1 and 3%) of ROP powder were added to cheese formulations, and the subsequent impact on the texture characteristics, sensory attributes, and phytochemical composition of the value-added cheeses was evaluated. The findings show that the addition of ROP powder improved the texture and the color of the cheeses, providing a visually appealing product. Additionally, adding the ROP powder significantly raised the amount of phytochemicals and antioxidant activity (17.08 ± 0.78 µmol TE/g dw for RCROP1, 24.55 ± 0.67 µmol TE/g dw for RCROP2) in the final product’s formulation. Moreover, adding powder to cheese is an effective way to increase the value of onion by-products and produce polyphenol-enriched cheese.
Research on Application of Fractional Calculus Operator in Image Underlying Processing
Authors: Huang Guo , Qin Hong-ying , Chen Qingli , Shi Zhanzhan , Jiang Shan , Huang Chenying
Published: January 2024

Fractional calculus extends traditional, integer-based calculus to include non-integer orders, offering a powerful tool for a range of engineering applications, including image processing. This work delves into the utility of fractional calculus in two crucial aspects of image processing: image enhancement and denoising. We explore the foundational theories of fractional calculus together with its amplitude–frequency characteristics. Our focus is on the effectiveness of fractional differential operators in enhancing image features and reducing noise. Experimental results reveal that fractional calculus offers unique benefits for image enhancement and denoising. Specifically, fractional-order differential operators outperform their integer-order counterparts in accentuating details such as weak edges and strong textures in images. Moreover, fractional integral operators excel in denoising images, not only improving the signal-to-noise ratio but also better preserving essential features such as edges and textures when compared to traditional denoising techniques. Our empirical results affirm the effectiveness of the fractional-order calculus-based image-processing approach in yielding optimal results for low-level image processing.
Hydrogels and Wound Healing: Current and Future Prospects
Authors: Gounden Varshan , Singh Moganavelli
Published: January 2024

The care and rehabilitation of acute and chronic wounds have a significant social and economic impact on patients and global health. This burden is primarily due to the adverse effects of infections, prolonged recovery, and the associated treatment costs. Chronic wounds can be treated with a variety of approaches, which include surgery, negative pressure wound therapy, wound dressings, and hyperbaric oxygen therapy. However, each of these strategies has an array of limitations. The existing dry wound dressings lack functionality in promoting wound healing and exacerbating pain by adhering to the wound. Hydrogels, which are commonly polymer-based and swell in water, have been proposed as potential remedies due to their ability to provide a moist environment that facilitates wound healing. Their unique composition enables them to absorb wound exudates, exhibit shape adaptability, and be modified to incorporate active compounds such as growth factors and antibacterial compounds. This review provides an updated discussion of the leading natural and synthetic hydrogels utilized in wound healing, details the latest advancements in hydrogel technology, and explores alternate approaches in this field. Search engines Scopus, PubMed, Science Direct, and Web of Science were utilized to review the advances in hydrogel applications over the last fifteen years.
Evolution of Armenian Surname Distribution in France between 1891 and 1990
Authors: Darlu Pierre , Chareille Pascal
Published: January 2024

The evolution of the Armenian presence in mainland France from 1891 to 1990 is described on the basis of an inventory of more than 7000 family names of Armenian origin extracted from the INSEE surname database. Several surname samplings are proposed, and parameters such as the number of different Armenian names, the number of births with these names and their proportions are used as descriptors for each of the 320 French arrondissements and the four successive 25-year periods between 1891 and 1990. Before 1915, Armenian surnames and births with these names are infrequent and almost exclusively located in Paris and the arrondissements of Marseille. From 1915 onwards, subsequent to the genocide in Turkey, the number of births and the diversity of Armenian surnames rose sharply until 1940, before stabilizing thereafter. The diaspora remains essentially centred in Paris, Lyon, and Marseille, with little regional extension around these poles.
Estimation of Genetic Diversity and Number of Unique Genotypes of Cassava Germplasm from Burkina Faso Using Microsatellite Markers
Authors: Soro Monique , Zida Serge Marie Felicien Wend-Pagnagdé , Somé Koussao , Tiendrébéogo Fidèle , Otron Daniel H. , Pita Justin S. , Néya James B. , Koné Daouda
Published: January 2024

Genetic diversity is very important in crop improvement. This study was carried out to assess the genetic diversity and the number of unique multilocus genotypes (MLGs) in a cassava collection in Burkina Faso. To achieve this objective, 130 cassava accessions were genotyped using 32 simple sequence repeat (SSR) markers. The results revealed that among these markers, twelve (12) were highly informative, with polymorphic information content (PIC) values greater than 0.50; twelve (12) were moderately informative, with PIC values ranging between 0.25 and 0.50; and eight (8) were not very informative, with PIC values lower than 0.25. A moderate level of genetic diversity was found for the population, indicated by the average expected heterozygosity (0.45) and the observed heterozygosity (0.48). About 83.8% of unique multilocus genotypes were found in the cassava collection, indicating that SSR markers seem to be most appropriate for MLG identification. Population structure analysis based on hierarchical clustering identified two subpopulations and the Bayesian approach suggested five clusters. Additionally, discriminant analysis of principal components (DAPC) separated the cassava accessions into 13 subpopulations. A comparison of these results and those of a previous study using single nucleotide polymorphisms (SNP) suggests that each type of marker can be used to assess the genetic structure of cassava grown in Burkina Faso.
Attentional Resources and Independence in Basic and Instrumental Activities of Daily Living in Individuals with Intellectual Disabilities
Authors: García-Pintor Beatriz , Morales-Rodríguez Francisco Manuel , Pérez-Mármol José Manuel
Published: January 2024

The relationship between attentional resources and functionality in individuals with intellectual disabilities (IDs) is clinically relevant. This study aimed to examine the possible relationship between the degree of ID and attentional resources, and to evaluate whether attentional resources predict the performance of basic and instrumental activities of daily living (ADL) in individuals with mild and moderate ID. This study, which employed a descriptive, cross-sectional, observational design, was conducted between July 2019 and May 2020. The sample consisted of 166 individuals divided into three groups: moderate ID, mild ID, and those without ID. These groups were compared for attentional functions (p < 0.001), obtaining an effect size ranging from medium to large. The results indicated that 40% of the variance in basic ADL performance was explained by the age of the participants, degree of disability, and sustained attention in individuals with ID. Additionally, 64% of the variance in instrumental ADL performance was explained by sustained, divided, and executive attention. Therefore, attentional resources appear to be associated with the performance of basic and instrumental ADL in individuals with mild and moderate ID.
Assessing the Effect of Plant Growth Stimulants and Retardants on Cyclamen “Halios F1 Salmon Rose” Cultivar
Authors: Bálint János , Benedek Klára , Csorba Artúr Botond
Published: January 2024

In Eastern Europe, the traditional marketing of cyclamen is in the period from the middle of February to the middle of March. The poor light of winter months and the higher number of plants (because of the reduction in heating costs) often result in elongated plants. To avoid this, it is recommended to use plant growth retardants. At the same time, another problem is that flowers do not rise from the level of rosette due to the unfavorable cultivation conditions. This can be solved with growth stimulants. In the experiment, we tested the effect of growth regulators on the growth of a frequently used variety of cyclamen. We used daminozide and paclobutrazol plant growth retardants for height control and gibberellic acid (GA) and benzyladenine (BAP) as growth promoters for increasing the number of flowers and the length of stems. The results show that daminozide and paclobutrazol are both effective for height control in cyclamen production.
Effects of the Cultivation Methods on the Sensory Quality and Phytochemical Profiles of Satsuma Mandarin (Citrus unshiu)
Authors: Jeong Sung-Man , Kim Dong-Shin , Kim Sang Suk , Kim Hyun-Jin
Published: January 2024

Citrus fruits have a distinctive flavor and can convey health benefits because of their unique phytochemicals. Phytochemical profiles are influenced by many factors, including variety and environmental growing conditions; however, the effect of the cultivation methods on the phytochemical profile of Satsuma mandarin (Citrus unshiu) has received little attention. In this study, we examined the relationships between the cultivation conditions, sensory quality, and phytochemical profiles of C. unshiu cultivated using four methods: open field, greenhouse, film mulching, and tunnel farming. The soil water content differed significantly between the cultivation methods and showed a strong positive correlation with sourness, bitterness, and astringency and a strong negative correlation with sweetness. The metabolites of C. unshiu were not associated with the soil water content but with the soil mineral content, including nitrogen (N+), phosphorus (P+), and potassium (K+). The soil P+ and K+ content was positively correlated with most secondary metabolites. The relative abundance of sugars did not differ significantly between the cultivation methods; however, the sweetness was higher under film mulching than under the other cultivation methods because of the suppression of sweetness by bitter compounds. We did not investigate the effect of other growing conditions, such as sunlight; however, the results improve our understanding of the effect of cultivation methods on the quality of C. unshiu and may inform crucial decisions concerning citrus cultivation.
Novel Fermentation Strategies of Strawberry Tree Arbutus unedo Fruits to Obtain High Nutritional Value Products
Authors: Ramires Francesca Anna , Durante Miriana , D’Antuono Isabella , Garbetta Antonella , Bruno Angelica , Tarantini Annamaria , Gallo Antonia , Cardinali Angela , Bleve Gianluca
Published: January 2024

The strawberry tree (Arbustus unedo) is a medicinal plant and an important source of biocompounds, potentially useful for pharmaceutical and chemical applications to prevent or treat several human diseases. The strawberry tree fruits have usually been used to produce traditional products such as jams and jellies and to obtain fermented alcoholic drinks, representing the most valuable derivative products. Other fermented products are potentially interesting for their nutritional value; however, the fermentation process needs to be controlled and standardized to obtain high-quality products/ingredients. In this work, we investigated two different fermentative procedures, using strawberry tree whole fruit and fruit paste as matrices inoculated with a selected starter strain of Saccharomyces cerevisiae LI 180-7. The physical, chemical, microbiological and nutritional properties of fermented products were evaluated, as well as their antioxidant activity. The new obtained fermented products are enriched in organic acids (acetic acid varied from 39.58 and 57.21 mg/g DW and lactic acid from 85.33 to 114.1 mg/g DW) and have better nutritional traits showing a higher amount of total polyphenols (phenolic acids, flavonoids and anthocyanins) that ranged from 1852 mg GAE/100 g DW to 2682 mg GAE/100 g DW. Also, the amount of isoprenoid increased ranging from 155.5 μg/g DW to 164.61 μg/g DW. In this regard, the most promising strategy seemed to be the fermentation of the fruit paste preparation; while the extract of fermented whole fruits showed the most powerful antioxidant activity. Finally, a preliminary attempt to produce a food prototype enriched in fermented strawberry tree fruits suggested the whole fruit fermented sample as the most promising from a preliminary sensory analysis.
Nanoparticulated WO3/NiWO4 Using Cellulose as a Template and Its Application as an Auxiliary Co-Catalyst to Pt for Ethanol and Glycerol Electro-Oxidation
Authors: Guimarães Munique G. , Macedo Julio L. , Linares José J. , Ghesti Grace F.
Published: January 2024

This work reports the use of cellulose as a template to prepare nanosized WO3 or NiWO4 and its application as a co-catalyst in the electro-oxidation of ethanol and glycerol. Microcrystalline cellulose was hydrolyzed with phosphotungstic acid (H3PW12O40) to prepare the nanocrystalline cellulose template. The latter was air-calcinated to remove the template and obtain nanometric WO3. Tungsten oxide was impregnated with Ni(NO3)2, which was subsequently air-calcinated to obtain the nanometric NiWO4. Elemental analysis confirmed the coexistence of nickel and tungsten, whereas thermal analysis evidenced a high thermal stability for these materials. The X-ray diffractograms displayed crystal facets of WO3 and, when Ni(II) was added, NiWO4. The transmission electron micrographs corroborated the formation of nanosized particles with average particle sizes in the range of 30 to 50 nm. Finally, to apply this material, Pt/WO3-C and Pt/WO3-NiWO4-C were prepared and used in ethanol and glycerol electro-oxidation in an alkaline medium, observing a promotional effect of the oxide and tungstate by reducing the onset potential and increasing the current density. These materials show great potential to produce clean electricity or green hydrogen, contributing to energetic transition.
Supplementary Low Far-Red Light Promotes Proliferation and Photosynthetic Capacity of Blueberry In Vitro Plantlets
Authors: Wang Yuting , Jiang Zhehao , Li Wenxiang , Yang Xiaolong , Li Chengming , Cai Dunxu , Pan Yunxue , Su Wei , Chen Riyuan
Published: January 2024

Far-red light exerts an important regulatory influence on plant growth and development. However, the mechanisms underlying far-red light regulation of morphogenesis and photosynthetic characteristics in blueberry plantlets in vitro have remained elusive. Here, physiological and transcriptomic analyses were conducted on blueberry plantlets in vitro supplemented with far-red light. The results indicated that supplementation with low far-red light, such as 6 μmol m−2 s−1 and 14 μmol m−2 s−1 far-red (6FR and 14FR) light treatments, significantly increased proliferation-related indicators, including shoot length, shoot number, gibberellin A3, and trans-zeatin riboside content. It was found that 6FR and 14 FR significantly reduced chlorophyll content in blueberry plantlets but enhanced electron transport rates. Weighted correlation network analysis (WGCNA) showed the enrichment of iron ion-related genes in modules associated with photosynthesis. Genes such as NAC, ABCG11, GASA1, and Erf74 were significantly enriched within the proliferation-related module. Taken together, we conclude that low far-red light can promote the proliferative capacity of blueberry plantlets in vitro by affecting hormone pathways and the formation of secondary cell walls, concurrently regulating chlorophyll content and iron ion homeostasis to affect photosynthetic capacity.
iPSC-Derived Endothelial Cells Reveal LDLR Dysfunction and Dysregulated Gene Expression Profiles in Familial Hypercholesterolemia
Authors: Zakharova Irina S. , Shevchenko Alexander I. , Arssan Mhd Amin , Sleptcov Aleksei A. , Nazarenko Maria S. , Zarubin Aleksei A. , Zheltysheva Nina V. , Shevchenko Vlada A. , Tmoyan Narek A. , Saaya Shoraan B. , Ezhov Marat V. , Kukharchuk Valery V. , Parfyonova Yelena V. , Zakian Suren M.
Published: January 2024

Defects in the low-density lipoprotein receptor (LDLR) are associated with familial hypercholesterolemia (FH), manifested by atherosclerosis and cardiovascular disease. LDLR deficiency in hepatocytes leads to elevated blood cholesterol levels, which damage vascular cells, especially endothelial cells, through oxidative stress and inflammation. However, the distinctions between endothelial cells from individuals with normal and defective LDLR are not yet fully understood. In this study, we obtained and examined endothelial derivatives of induced pluripotent stem cells (iPSCs) generated previously from conditionally healthy donors and compound heterozygous FH patients carrying pathogenic LDLR alleles. In normal iPSC-derived endothelial cells (iPSC-ECs), we detected the LDLR protein predominantly in its mature form, whereas iPSC-ECs from FH patients have reduced levels of mature LDLR and show abolished low-density lipoprotein uptake. RNA-seq of mutant LDLR iPSC-ECs revealed a unique transcriptome profile with downregulated genes related to monocarboxylic acid transport, exocytosis, and cell adhesion, whereas upregulated signaling pathways were involved in cell secretion and leukocyte activation. Overall, these findings suggest that LDLR defects increase the susceptibility of endothelial cells to inflammation and oxidative stress. In combination with elevated extrinsic cholesterol levels, this may result in accelerated endothelial dysfunction, contributing to early progression of atherosclerosis and other cardiovascular pathologies associated with FH.
Circulating Interleukins-33 and -37 and Their Associations with Metabolic Syndrome in Arab Adults
Authors: Amer Osama E. , Sabico Shaun , Khattak Malak N. K. , Alnaami Abdullah M. , Saadawy Gamal M. , Al-Daghri Nasser M.
Published: January 2024

Interleukins (ILs) are a group of cytokines known to have immunomodulatory effects; they include ILs–33 and –37 whose emerging roles in the pathogenesis of metabolic syndrome (MetS) remain under investigated. In this study, we compared circulating IL–33 and IL–37 in Arab adults with and without MetS to determine its associations with MetS components. A total of 417 Saudi participants (151 males, 266 females; mean age ± SD 41.3 ± 9.0 years; mean body mass index ± SD 30.7 ± 6.3 kg/m2) were enrolled and screened for MetS using the ATP III criteria. Anthropometrics and fasting blood samples were taken for the assessment of fasting glucose and lipids. Circulating levels of IL–33 and IL–37 were measured using commercially available assays. The results showed higher levels of serum IL–33 and IL–37 in participants with MetS than those without (IL-33, 3.34 3.42 (2.3–3.9) vs. (1–3.9), p = 0.057; IL-37, 5.1 (2.2–8.3) vs. 2.9 (2.1–6.1), p = 0.01). Additionally, having elevated levels of IL–33 was a risk factor for hypertension, low HDL-c, and hypertriglyceridemia. A stratification of the participants according to sex showed that males had higher IL-33 levels than females [3.7 (3.0–4.1) vs. 3.15 (1.4–3.8), p < 0.001], while females had higher levels of IL–37 than males [3.01 (2.2–7.0) vs. 2.9 (2.1–5.6), p = 0.06]. In conclusion, the presence of MetS substantially alters the expression of ILs–33 and -37. IL-33 in particular can be potentially used as a therapeutic target to prevent MetS progression. Longitudinal and interventional studies are warranted to confirm present findings.
Enhanced Enzymatic Synthesis of Puerarin Palmitate with Different Acyl Donors for Lipid Solubility Improvement
Authors: Lee Seungmee , Shin Hyeonmi , Bae Jihyun , Lee Taek , Kim Minji , Jeon Heung Bae , Lee Kang Hyun , Yoo Hah Young , Park Chulhwan
Published: January 2024

Puerarin is a flavonoid known as a natural antioxidant found in the root of Pueraria robata. Its antioxidant, anticancer, and anti-inflammatory effects have attracted attention as a potential functional ingredient in various bioindustries. However, puerarin has limited bioavailability owing to its low lipid solubility and stability. Acylation is proposed as a synthesis method to overcome this limitation. In this study, lipase-catalyzed acylation of puerarin and various acyl donors was performed, and the enzymatic synthetic condition was optimized. Under the condition (20 g/L of Novozym 435, palmitic anhydride, 1:15, 40 °C, tetrahydrofuran (THF)), the synthesis of puerarin ester achieved a significantly high conversion (98.97%) within a short time (3 h). The molecule of the synthesized puerarin palmitate was identified by various analyses such as liquid chromatography–mass spectrometry (LC–MS), Fourier-transform infrared spectroscopy (FT-IR), and carbon-13 nuclear magnetic resonance (13C NMR). The lipid solubility and the radical scavenging activity were also evaluated. Puerarin palmitate showed a slight decrease in antioxidant activity, but lipid solubility was significantly improved, improving bioavailability. The high conversion achieved for puerarin esters in this study will provide the foundation for industrial applications.
Y-Box Binding Protein 1: Unraveling the Multifaceted Role in Cancer Development and Therapeutic Potential
Authors: Dinh Ngoc Thi Minh , Nguyen Tuan Minh , Park Mi Kyung , Lee Chang Hoon
Published: January 2024

Y-box binding protein 1 (YBX1), a member of the Cold Shock Domain protein family, is overexpressed in various human cancers and is recognized as an oncogenic gene associated with poor prognosis. YBX1’s functional diversity arises from its capacity to interact with a broad range of DNA and RNA molecules, implicating its involvement in diverse cellular processes. Independent investigations have unveiled specific facets of YBX1’s contribution to cancer development. This comprehensive review elucidates YBX1’s multifaceted role in cancer across cancer hallmarks, both in cancer cell itself and the tumor microenvironment. Based on this, we proposed YBX1 as a potential target for cancer treatment. Notably, ongoing clinical trials addressing YBX1 as a target in breast cancer and lung cancer have showcased its promise for cancer therapy. The ramp up in in vitro research on targeting YBX1 compounds also underscores its growing appeal. Moreover, the emerging role of YBX1 as a neural input is also proposed where the high level of YBX1 was strongly associated with nerve cancer and neurodegenerative diseases. This review also summarized the up-to-date advanced research on the involvement of YBX1 in pancreatic cancer.
Predicting an Optimal Medication/Prescription Regimen for Patient Discordant Chronic Comorbidities Using Multi-Output Models
Authors: Sharma Ichchha Pradeep , Nguyen Tam V. , Singh Shruti Ajay , Ongwere Tom
Published: January 2024

This paper focuses on addressing the complex healthcare needs of patients struggling with discordant chronic comorbidities (DCCs). Managing these patients within the current healthcare system often proves to be a challenging process, characterized by evolving treatment needs necessitating multiple medical appointments and coordination among different clinical specialists. This makes it difficult for both patients and healthcare providers to set and prioritize medications and understand potential drug interactions. The primary motivation of this research is the need to reduce medication conflict and optimize medication regimens for individuals with DCCs. To achieve this, we allowed patients to specify their health conditions and primary and major treatment concerns, for example, costs of medication, interactions with current drugs, and weight gain. Utilizing data gathered from MTurk and Qualtrics, we gained insights into healthcare providers’ strategies for making/customizing medication regimens. We constructed a dataset and subsequently deployed machine learning algorithms to predict optimal medication regimens for DCC patients with specific treatment concerns. Following the benchmarking different models, Random forest emerged as the top performer, achieving an accuracy of 0.93. This research contributes significantly to the enhancement of decision-making processes, empowers patients to take a more active role in their healthcare, and promotes more informed and productive discussions between patients and their care teams.
Heartfelt Breakthroughs: Elevating Quality of Life with Cutting-Edge Advances in Heart Failure Treatment
Authors: Devadoss Ramprakash , Dhillon Gagandeep , Sharma Pranjal , Verma Ram Kishun , Munjal Ripudaman , Kashyap Rahul
Published: January 2024

Heart failure is a cardiovascular condition, leading to fatigue, breathlessness, and fluid retention. It affects around 56 million people globally and is a leading cause of hospitalization and mortality. Its prevalence is rising due to aging populations and lifestyle factors. Managing heart failure demands a multidisciplinary approach, encompassing medications, lifestyle modifications, and often medical devices or surgeries. The treatment burden is substantial, impacting patients’ daily lives and straining healthcare systems. Improving early detection, novel therapies, and patient education are crucial for alleviating the burden and enhancing the quality of life. There are notable advancements in the field of heart failure treatment and prevention. We will discuss significant pharmacological and device advances related to heart failure, including angiotensin receptor–neprilysin inhibitor, sodium–glucose co-transporter inhibition, glucagon-like peptide-1 agonist, cardiac resynchronization therapy, cardiac contractility modulation, mechanical circulatory support devices, and transcatheter valve interventions. We will also review novel therapies on the horizon, emerging technologies like CRISPR-based treatments for genetic anomalies, and the involvement of artificial intelligence in heart failure detection and management.
Evaluation of Conventional Cardiovascular Risk Factors and Ordinal Coronary Artery Calcium Scoring in a Lung Cancer Screening Cohort
Authors: Kasprzyk Piotr , Undrunas Aleksandra , Dziadziuszko Katarzyna , Dziedzic Robert , Kuziemski Krzysztof , Szurowska Edyta , Rzyman Witold , Zdrojewski Tomasz
Published: January 2024

(1) Background: Lung cancer screening (LCS) consists of low-dose computed tomography (LDCT) results to reduce lung cancer-related mortality. The LCS program has a unique opportunity to impact CVD mortality by providing tools for CVD risk assessment and implementing preventative strategies. In this study, we estimated standardized CVD risk (SCORE) and assessed the prevalence of coronary artery calcium (CAC) in a Polish LCS cohort. (2) Methods: In this observational study, 494 LCS participants aged 50–79 years with a cigarette smoking history of at least 30 pack-years were included. Medical history, anthropometric measurements, blood pressure measurements, serum glucose, and cholesterol levels were assessed in one visit. CVD risk assessment using SCORE tables was performed. The results were compared to the general population (NATPOL 2011 study). On LDCT scans, CAC was classified using an Ordinal Score ranging from 0 to 12. (3) Results: The prevalence of classic cardiovascular risk factors was very high. Among study participants, 83.7% of men and 40.7% of women were classified with a very high CVD SCORE risk (>10%). CAC was reported in 190 (47%) participants. Calcification was categorized as severe (CAC ≥ 4) in 84 (21%) participants. (4) Conclusions: Due to the high cardiovascular risk, intensive preventive strategies are recommended for LCS participants.
Telerehabilitation for Neurological Motor Impairment: A Systematic Review and Meta-Analysis on Quality of Life, Satisfaction, and Acceptance in Stroke, Multiple Sclerosis, and Parkinson’s Disease
Authors: Federico Sara , Cacciante Luisa , Cieślik Błażej , Turolla Andrea , Agostini Michela , Kiper Pawel , Picelli Alessandro
Published: January 2024

Telerehabilitation (TR) seems to be a viable and feasible solution to face the rehabilitative challenges posed by neurological impairments and to improve patients’ quality of life (QoL). This review aims to synthesize and analyze the evidence on the impact of physiotherapy intervention through TR on QoL in patients with stroke, Parkinson’s disease (PD), and multiple sclerosis (MS), together with an evaluation of their satisfaction and technology acceptance levels. Through a systematic search of the literature and a screening process, treatment effects were assessed with meta-analyses using the standardized mean difference, setting the confidence interval at 95%. We included 28 studies in the review, which were analyzed for methodological quality, whereas 16 studies were included in the meta-analyses. The results suggest a significant improvement in QoL in patients who underwent TR. We were unable to perform analyses for satisfaction and technology acceptance outcomes due to insufficient data. Overall, motor TR has a positive impact on the QoL of patients with neurological diseases, especially in stroke patients; although caution is needed in the interpretation of the results due to the high heterogeneity found. For PD and MS, TR seems to yield comparable results to in-person treatment.
The Value of Next-Generation Sequencing in Diagnosis and Therapy of Critically Ill Patients with Suspected Bloodstream Infections: A Retrospective Cohort Study
Authors: Overbeek Remco , Leitl Christoph J. , Stoll Sandra E. , Wetsch Wolfgang A. , Kammerer Tobias , Mathes Alexander , Böttiger Bernd W. , Seifert Harald , Hart Dominique , Dusse Fabian
Published: January 2024

Bloodstream infection (BSI), a frequent cause of severe sepsis, is a life-threatening complication in critically ill patients and still associated with a high mortality rate. Rapid pathogen identification from blood is crucial for an early diagnosis and the treatment of patients with suspected BSI. For this purpose, novel diagnostic tools on the base of genetic analysis have emerged for clinical application. The aim of this study was to assess the diagnostic value of additional next-generation sequencing (NGS) pathogen test for patients with suspected BSI in a surgical ICU and its potential impact on antimicrobial therapy. In this retrospective single-centre study, clinical data and results from blood culture (BC) and NGS pathogen diagnostics were analysed for ICU patients with suspected BSI. Consecutive changes in antimicrobial therapy and diagnostic procedures were evaluated. Results: 41 cases with simultaneous NGS and BC sampling were assessed. NGS showed a statistically non-significant higher positivity rate than BC (NGS: 58.5% (24/41 samples) vs. BC: 21.9% (9/41); p = 0.056). NGS detected eight different potentially relevant bacterial species, one fungus and six different viruses, whereas BC detected four different bacterial species and one fungus. NGS results affected antimicrobial treatment in 7.3% of cases. Conclusions: NGS-based diagnostics have the potential to offer a higher positivity rate than conventional culture-based methods in patients with suspected BSI. Regarding the high cost, their impact on anti-infective therapy is currently limited. Larger randomized prospective clinical multicentre studies are required to assess the clinical benefit of this novel diagnostic technology.
Hand-Held Echocardiography by Advanced Practice Providers in Patients with Congestive Heart Failure
Authors: Tagle-Cornell Maria Cecilia , Novais Barbara S. , Wen Songnan , Shipman Justin N. , Mandale Deepa R. , Flom Andrew P. , Sahnan Sandeep K. , Kriz Lindsey M. , Alland Michelle L. , Bird Christen W. , Naqvi Tasneem Z.
Published: January 2024

Objectives: The performed hand-held echocardiography (HHE) was evaluated and interpreted by trained advanced practice providers (APPs) on hospitalized CHF patients for image quality and interpretation by comparing with expert echocardiographer and SE findings. Background: Congestive heart failure (CHF) is associated with increased hospital admissions and mortality. While a standard echocardiogram (SE) is the gold standard for cardiac assessment, it is not readily available. Hospitalized CHF patients require rapid assessment for expedited treatment. Methods: Over 6 months, five trained APPs performed HHE on hospitalized CHF patients and interpreted: (a) left ventricular (LV) size, (b) LV ejection fraction (LVEF), and (c) right atrial pressure (RAP). The study echocardiographer reviewed and blindly interpreted the HHE images and compared them with APPs and SE findings. Kappa statistics determined the degree of agreement between APPs and the study echocardiographer’s interpretation of the HHE images and SE. Results: A total of 80 CHF patients (age 73 ± 14 years, 58% males; LVEF (by SE) 45 ± 19%; 36.3% body mass indexes ≥ 30 kg/m2) were enrolled. HHE interpretation by APPs had a good agreement for LVEF (kappa 0.79) with the study echocardiographer and SE (kappa 0.74) and a good agreement for RAP (kappa 0.67) with the study echocardiographer. The correlation between the absolute LVEF interpretation by the study echocardiographer on HHE and SE was r = 0.88 (p < 0.0001). Conclusions: Trained APPs obtained diagnostic-quality HHE images and interpreted the LV function and RAP in CHF patients in good agreement with the study echocardiographer. LVEF by HHE correlated with LVEF by SE. Our study suggests trained APPs can use HHE to evaluate LVEF and RAP in CHF patients, leading to expedited and optimized treatment.
Antepartum Fetal Surveillance and Optimal Timing of Delivery in Diabetic Women: A Narrative Review
Authors: Braverman-Poyastro Alan , Suárez-Rico Blanca Vianey , Borboa-Olivares Héctor , Espino y Sosa Salvador , Torres-Torres Johnatan , Arce-Sánchez Lidia , Martínez-Cruz Nayeli , Reyes-Muñoz Enrique
Published: January 2024

Antepartum fetal surveillance (AFS) is essential for pregnant women with diabetes to mitigate the risk of stillbirth. However, there is still no universal consensus on the optimal testing method, testing frequency, and delivery timing. This review aims to comprehensively analyze the evidence concerning AFS and the most advantageous timing for delivery in both gestational and pregestational diabetes mellitus cases. This review’s methodology involved an extensive literature search encompassing international diabetes guidelines and scientific databases, including PubMed, MEDLINE, Google Scholar, and Scopus. The review process meticulously identified and utilized pertinent articles for analysis. Within the scope of this review, a thorough examination revealed five prominent international guidelines predominantly addressing gestational diabetes. These guidelines discuss the utility and timing of fetal well-being assessments and recommendations for optimal pregnancy resolution timing. However, the scarcity of clinical trials directly focused on this subject led to a reliance on observational studies as the basis for most recommendations. Glucose control, maternal comorbidities, and the medical management received are crucial in making decisions regarding AFS and determining the appropriate delivery timing.
Assessment of Quality of Life in Patients with Chronic Anal Fissures: A 1-Year Follow-Up Study before and after Botulinum Toxin (Botox) Injection
Authors: Burtic Sonia-Roxana , Castiglione Luca , Murariu Marius , Rosca Ovidiu , Dumitru Catalin , Neagoe Octavian
Published: January 2024

This longitudinal study aimed to assess the quality of life in patients with anal fissures treated with botulinum toxin (Botox) injections over a one-year period. The study hypothesized that Botox injections would significantly improve quality of life and that these improvements would be sustained over a year. Conducted as a cross-sectional study, it assessed adults diagnosed with chronic anal fissures unresponsive to conventional treatments. Participants received 25 U of Botox in two sessions and their quality of life was assessed using the WHOQOL-BREF, COPE-60, Hospital Anxiety and Depression Scale (HADS), and SF-36 surveys. Data were collected at baseline six months and one year post-treatment. The study involved 113 patients, with a mean age of 38.1 years. Significant improvements were observed in the WHOQOL-BREF scores across all domains from baseline to 12 months (physical domain: 49.4 ± 10.5 to 70.2 ± 10.6, p < 0.001; mental domain: 34.8 ± 11.2 to 61.9 ± 11.5, p < 0.001). SF-36 scores also showed significant enhancements in physical and mental health components (physical: 44.3 ± 7.5 to 56.9 ± 5.9, p < 0.001; mental: 41.1 ± 7.2 to 54.4 ± 6.3, p < 0.001). Additionally, significant improvements were noted in patient perception on quality of life from the perspective of various aspects including physical discomfort, pain management, and mood and emotional well-being. The study demonstrated that Botox injections significantly improved the quality of life in patients with chronic anal fissures, with sustained benefits observed over a year. These findings suggest Botox as an effective treatment modality for enhancing life quality in patients with this condition, highlighting the potential for broader applications in managing chronic anal fissures.
The Association of Nanostructured Carbonated Hydroxyapatite with Denatured Albumin and Platelet-Rich Fibrin: Impacts on Growth Factors Release and Osteoblast Behavior
Authors: de Lima Barbosa Renata , Rodrigues Santiago Rocha Neilane , Stellet Lourenço Emanuelle , de Souza Lima Victor Hugo , Mavropoulos Elena , Mello-Machado Rafael Coutinho , Spiegel Carolina , Mourão Carlos Fernando , Alves Gutemberg Gomes
Published: January 2024

Platelet-rich Fibrin (PRF), a second-generation blood concentrate, offers a versatile structure for bone regeneration due to its composition of fibrin, growth factors, and cytokines, with adaptations like denatured albumin-enriched with liquid PRF (Alb-PRF), showing potential for enhanced stability and growth factor dynamics. Researchers have also explored the combination of PRF with other biomaterials, aiming to create a three-dimensional framework for enhanced cell recruitment, proliferation, and differentiation in bone repair studies. This study aimed to evaluate a combination of Alb-PRF with nanostructured carbonated hydroxyapatite microspheres (Alb-ncHA-PRF), and how this association affects the release capacity of growth factors and immunomodulatory molecules, and its impact on the behavior of MG63 human osteoblast-like cells. Alb-PRF membranes were prepared and associated with nanocarboapatite (ncHA) microspheres during polymerization. MG63 cells were exposed to eluates of both membranes to assess cell viability, proliferation, mineralization, and alkaline phosphatase (ALP) activity. The ultrastructural analysis has shown that the spheres were shattered, and fragments were incorporated into both the fibrin mesh and the albumin gel of Alb-PRF. Alb-ncHA-PRF presented a reduced release of growth factors and cytokines when compared to Alb-PRF (p < 0.05). Alb-ncHA-PRF was able to stimulate osteoblast proliferation and ALP activity at lower levels than those observed by Alb-PRF and was unable to positively affect in vitro mineralization by MG63 cells. These findings indicate that the addition of ncHA spheres reduces the biological activity of Alb-PRF, impairing its initial effects on osteoblast behavior.
Phase Composition, Microstructure and Mechanical Properties of Zr57Cu15Ni10Nb5 Alloy Obtained by Selective Laser Melting
Authors: Khmyrov Roman S. , Korotkov Andrey , Gridnev Mikhail , Podrabinnik Pavel , Tarasova Tatiana V. , Gusarov Andrey V.
Published: January 2024

Zr57Cu15Ni10Nb5 (more known as Vit-106) is a promising zirconium-based alloy with a high glass-forming ability, and belongs to the so-called bulk metallic glasses (BMG). Workpieces with a size of around one centimeter in all three dimensions can be obtained from a BMG alloy by casting. However, further increasing the cast size decreases the cooling rate and thus induces crystallization. Selective laser melting (SLM) is a well-known technique to overcome size limitations for BMGs because a workpiece is built by the addition of multiple melt portions in which the cooling rate is kept above the critical one. Currently, BMG parts obtained by SLM suffer from partial crystallization. The present work studies the influence of SLM process parameters on the partial crystallization of Vit-106 by metallography and the influence of the microstructure on mechanical properties by microhardness and wear resistance testing. Submicron crystalline inclusions are observed in an amorphous matrix of a Vit-106 alloy obtained by SLM. The size and the concentration of the inclusions can be controlled by varying the laser scanning speed. It is shown that submicron crystalline inclusions formed in the amorphous matrix during SLM can favorably affect microhardness and wear resistance.
Vickers Hardness Mechanical Models and Thermoplastic Polymer Injection-Molded Products’ Static Friction Coefficients
Authors: Takayama Tetsuo
Published: January 2024

The surface mechanical properties of thermoplastics are crucially important for evaluating molded products’ vulnerability to scratching. Because surface mechanical properties reflect material performance directly in terms of durability and frictional behavior, understanding and modeling them is important for industry and research. This emphasizes the surface mechanical properties of Vickers hardness and the static friction coefficient, with attempts to model them as functions of stress at yield initiation. Vickers hardness can be related to the compressive stress at yield initiation. The static friction coefficient can be modeled as a function of the surface shear strength and Vickers hardness. This research has improved our understanding of thermoplastics’ surface mechanical properties and has enabled the prediction of the scratch performance of molded products and the provision of effective indicators for material design.
Cooperative Maritime Search of Multi-Ship Based on Improved Robust Line-of-Sight Guidance
Authors: Guo Weili , Liu Cheng , Sun Ting
Published: January 2024

In this paper, an improved robust line-of-sight (RLOS) guidance-based fuzzy sliding mode controller is presented to control underactuated ships to conduct the cooperative maritime search operation under the presented improved creeping line search method. First, considering that the ship cannot perform turning with corners, an improved creeping line search method is presented by integrating the Bezier method into the traditional creeping line search method to smooth the transition points with corners and employing the cubic spline interpolation method to generate continuous reference paths. Second, an improved RLOS guidance method is presented for the first time by exploring the idea of robust adaptive control to mitigate the chattering effect of the RLOS guidance. Third, the fuzzy logic system with approximate ability is integrated into the design of sliding mode controller to handle unknown nonlinear model dynamics and environmental disturbances. Finally, an improved RLOS guidance-based fuzzy sliding mode controller is presented. The closed-loop stability is guaranteed by the Lyapunov theorem. Comparative simulations are conducted to illustrate the advantages and verify the effectiveness of the presented method.