bluuebunny/crossref_metadata_2025 huggingface dataset:
Malignancies After Lung Transplantation
Authors: Stenman Caroline , Wallinder Andreas , Holmberg Erik , Karason Kristjan , Magnusson Jesper , Dellgren Göran
Published: September 2024

Graphical Abstract
Use of Phenomics in the Selection of UAV-Based Vegetation Indices and Prediction of Agronomic Traits in Soybean Subjected to Flooding
Authors: Lima Charleston dos Santos , Junior Darci Francisco Uhry , Carvalho Ivan Ricardo , Bredemeier Christian
Published: September 2024

Flooding is a frequent environmental stress that reduces soybean growth and grain yield in many producing areas in the world, such as the United States, Southeast Asia, and Southern Brazil. In these regions, soybean is frequently cultivated in lowland areas in crop rotation with rice, which provides numerous technical, economic, and environmental benefits. In this context, the identification of the most important spectral variables for the selection of more flooding-tolerant soybean genotypes is a primary demand within plant phenomics, with faster and more reliable results enabled using multispectral sensors mounted on unmanned aerial vehicles (UAVs). Accordingly, this research aimed to identify the optimal UAV-based multispectral vegetation indices for characterizing the response of soybean genotypes subjected to flooding and to test the best linear model fit in predicting tolerance scores, relative maturity group, biomass, and grain yield based on phenomics analysis. Forty-eight soybean cultivars were sown in two environments (flooded and non-flooded). Ground evaluations and UAV-image acquisition were conducted at 13, 38, and 69 days after flooding and at grain harvest, corresponding to the phenological stages V8, R1, R3, and R8, respectively. Data were subjected to variance component analysis and genetic parameters were estimated, with stepwise regression applied for each agronomic variable of interest. Our results showed that vegetation indices behave differently in their suitability for more tolerant genotype selection. Using this approach, phenomics analysis efficiently identified indices with high heritability, accuracy, and genetic variation (>80%), as observed for MSAVI, NDVI, OSAVI, SAVI, VEG, MGRVI, EVI2, NDRE, GRVI, BNDVI, and RGB index. Additionally, variables predicted based on estimated genetic data via phenomics had determination coefficients above 0.90, enabling the reduction in the number of important variables within the linear model.
Physiological Indexes in Seed Germination and Seedling Growth of Rangpur Lime (Citrus limonia L. Osbeck) under Plant Growth Regulators
Authors: Domingues Neto Francisco José , Carneiro Débora Cavalcante dos Santos , Putti Fernando Ferrari , Rodrigues João Domingos , Tecchio Marco Antonio , Leonel Sarita , Silva Marcelo de Souza
Published: September 2024

The propagation of citrus seedlings is accomplished through grafting, utilizing seeds for the production of rootstocks. The germination of certain seeds may be low and uneven, complicating the production of high-quality seedlings. The use of plant growth regulators (PGRs) is a viable alternative to improve the quality of seedling production, as these compounds can break dormancy, control the hydrolysis of reserves, induce cell division, and regulate permeability and protein functions. This study aimed to evaluate the germination of seeds and the growth of Rangpur lime (Citrus limonia L. Osbeck) seedlings under the influence of imbibition in solutions of gibberellic acid (GA3) and a combination of GA4+7 + 6Benzyladenine. The experiment was conducted under controlled laboratory and greenhouse conditions, using a completely randomized design in a 2 × 5 factorial scheme, with two types of plant regulators (GA3 and GA4+7 + 6BA) at five concentrations (0, 250, 500, 750, and 1000 mg L−1 a.i.). Quantitative and qualitative variables were evaluated, ranging from seed germination to seedling development and formation, including germination percentage and speed index, fresh and dry biomass of roots and shoots, enzymatic activity, and gas exchange. The results indicate that GA3 significantly accelerates the germination process of Rangpur lime cv. Santa Cruz seeds and promotes better seedling growth and development, resulting in vigorous seedlings. These findings demonstrate that the application of PGRs, particularly GA3, can substantially enhance the propagation efficiency of citrus rootstocks, offering a practical solution for improving the uniformity and quality of seedling production in commercial settings.
Green Manure Mediated Improvement in Saline Soils in China: A Meta-Analysis
Authors: Li Yinjuan , Zhao Weiliang , Zhu Haojie , Jia Xuxia
Published: September 2024

The application of green manure is a traditional and valuable practice to improve the fertility of saline soil. However, the impact of environmental factors, green manure types and returning methods on the changes in soil fertility and soil salinity remain poorly quantified at a large scale. In the present study, we conducted a meta-analysis to generate a comprehensive evaluation of the effects of green manure on soil organic carbon (SOC), soil salt content, and soil nutrients compared to bare soil in China. The results showed that compared with bare soil, green manure planting could significantly increase the SOC content of saline soil, reduce salt content, and improve the soil total nitrogen (N), soil available phosphorus (P) and soil available potassium (K) contents. On average, green manure significantly enhanced SOC by 34.82% (percentage change), soil total N by 32.23%, soil available P by 34.34% and soil available K by 17.43%, while reducing soil salt content by 47.75%, compared to bare soil. In areas with a mean annual temperature (MAT) of <10 °C or a mean annual precipitation (MAP) of 200–400 mm, green manure had the largest increase in SOC, soil total N, soil available P, and soil available K. The smallest increases were observed in areas with an MAT above 15 °C and MAP greater than 800 mm. Green manure types influenced the improvement effect of green manure on saline soil. Green manure mixtures were more conducive to increases in SOC, while the increases in soil total N resulting from mixed green manure were lower in comparison to those from both legumes and non-legumes. In addition, the initial salt content, experimental years, and returning method influenced the improvement effect of green manure on saline soil. Therefore, this meta-analysis identified green manure as a promising practice for significantly improved saline soil in China.
A Whole Range of Cattle—An Isotopic Perspective on Roman Animal Husbandry in Lower Austria and Burgenland (Austria)
Authors: Kunst Günther Karl , Horacek Micha
Published: September 2024

In this study, we try to combine traditional archaeozoological biometry, based on outer bone measurements, with stable isotope analyses of bone collagen. Right from the start of archaeozoological research in Central and Western Europe, the important size variability in Roman domestic cattle has puzzled scholars. According to an established view, these differences in bone size are attributed either to the simultaneous presence of different types or even breeds or to the result of crossbreeding of smaller, native, and larger Roman cattle. Likewise, the episodic import of large-sized animals has been considered. First, we selected thirty proximal phalanges of cattle from three sites including five archaeological contexts from eastern Austria (Roman provinces of Noricum and Pannonia). The bone sample comprised the whole hitherto observed metric variability in Roman provincial cattle, and we tried to include minimal and maximal specimens. The results from stable isotope analyses (δ15N, δ13C, δ18O, δ2H) carried out on thirty proximal phalanges indicated that isotope signals were rather site-specific and, generally, not related to bone size. Therefore, we conclude that at least in the area investigated, small and large cattle types were raised and herded in the same areas and not spatially separated. There are, however, uncertain indicators of intra-site differences in isotope signals related to bone size, which should be checked on much larger sample sets.
Protein Expression, Amplification, and Mutation of HER2 Gene in Canine Primary Pulmonary Adenocarcinomas: Preliminary Results
Authors: Brunetti Barbara , de Biase Dario , Millanta Francesca , Muscatello Luisa Vera , Di Oto Enrico , Marchetti Roberta , Laddaga Ester Lidia , De Leo Antonio , Tallini Giovanni , Bacci Barbara
Published: September 2024

Recently, human epidermal growth factor receptor 2 (HER2) has emerged as a therapeutic target of interest for non-small-cell lung cancer in humans. The role of HER2 in canine pulmonary adenocarcinomas is poorly documented. To address this gap, this study employed three methodologies: immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and next-generation sequencing (NGS) to investigate the protein expression, gene amplification, and mutation of HER2 in 19 canine primary pulmonary adenocarcinomas. By IHC, 3 out of 19 cases were overexpressed 3+, 6 were 2+, and 10 were negative. With FISH, 2 cases were amplified (12.5%), 3 were inadequate for the analyses, and the others were non-amplified. With NGS, seven cases were inadequate. All other cases were wild-type, except for one IHC 3+ case, which was amplified with FISH and with a specific mutation already described in human pulmonary adenocarcinoma, V659E. This mutation is probably sensitive to tyrosine kinase inhibitory drugs. These results are similar to those in human medicine and to the few data in the literature on canine lung carcinomas; the presence of 12.5% of amplified cases in dogs lays the foundation for future targeted drugs against HER2 alterations.
Antimicrobial Susceptibility of Commensal Escherichia coli from Pig Fecal Samples and Enhanced Sensitivity for Direct Detection of the blaCTX-M Gene by Nested PCR
Authors: Suchanta Nutchaba , Ullah Naeem , Santanirand Pitak , Am-In Nutthee , Chaichanawongsaroj Nuntaree
Published: September 2024

The commensal Escherichia coli in the gut of pigs is a major reservoir of antimicrobial resistance and can result in possible transmission to humans through the food chain. Direct detection of E. coli from fecal samples is challenging and can be used as a bioindicator of antimicrobial resistance. This study aimed to compare the antimicrobial susceptibility profiles in commensal E. coli from antibiotic- and nonantibiotic-using pig farms and developed the direct detection of ESBL genes in pig fecal samples using nested PCR (nPCR) and multiplex PCR (mPCR) techniques. All direct genotypic results were validated with the results of PCR sequencing of isolated E. coli colonies. The ESBL-producing E. coli were found in 98.6% (145 isolates) and 96.6% (144 isolates) of antibiotic-using and nonantibiotic-using farms, respectively, predominantly CTX-M-55. The nPCR decreased the limit of detection (LOD) from sPCR about 100 times, and the lower LODs of 102, 101, and 1 CFU/mL were reached after incubating samples in an enrichment medium for 2, 4, and 8 h, respectively. The mPCR, sPCR, and nPCR techniques showed sensitivities of 30.15%, 69.85%, and 91.91%, respectively, compared to PCR sequencing. The stability and recycling of ESBL genes were independent of antibiotic usage in commensal E. coli originating in pig farms.
Experimental Study of the Stress State of a Polymer Composite in a State of Compression
Authors: Ishchenko Anatoliy , Kravchenko Volodymyr , Arustamian Artem , Rassokhin Dmytro , Seibert Dimitrij , Nosovska Olena , Böhm Robert , Kapustin Stanislav
Published: September 2024

Long-term operation of the supporting surfaces of large-sized parts, in particular tubular units of thermal power plants, leads to the destruction of the contact surfaces. Moisture penetrates into the formed discontinuities, and the vibrations present in the equipment in use rapidly increase the gap, reaching values of 10–15 mm. The authors of this article proposed the application of a composite layer of multimetal 1018 material without performing additional preparatory operations, ensuring the mandatory penetration of the material into the body of the supporting surface. This depth provides additional stability by maintaining boundary conditions. To determine the rational thickness of the composite layer, mathematical modeling of static loading of samples with different thicknesses in a wide range of values (from 2 mm to 12 mm) was performed. It was determined that the effective implementation of the developed technology was possible due to an increase in the load-bearing capacity of the composite material by creating additional grooves, or artificially creating grooves by welding, in the body of the part with a depth of 2.5–3 mm. The optimal excess of the composite was 1.0–1.5 mm. The proposed technology increases the stability of the composite layer up to three times and allows restoration without the use of mechanical treatment. The increase in the maximum stress values was 770 MPa, compared to the standard technology of 205 MPa.
Habitat Use of the Hen Harrier (Circus cyaneus) during the Breeding Season in Spain
Authors: Maeso Sara , Morollón Sara , García-Macía Jorge , Lee Simon , Urios Vicente
Published: September 2024

Raptors usually show use for a particular habitat to settle during the breeding period. In this study, we aimed to study the habitat use of the breeding populations of Hen Harrier (Circus cyaneus) in Spain, which represents the southern distribution limit of the species. We used GPS/GSM data from 17 individuals during 21 breeding periods. We analysed space and habitat use using 95% kernel density estimators to obtain home ranges during the breeding period. Then, we performed a third-order habitat selection analysis, extracting the percentage of locations on each land use and comparing it with random points generated within each home range. Furthermore, an Agricultural Use Index was created to classify harriers with agricultural or forest use depending on habitat. The results showed that harriers had a general use for agricultural land and, to a lesser extent, habitats with natural vegetation scrubs. Nest location was key to habitat selection, and these uses varied with latitude, with natural vegetation being chosen as the nesting habitat in the north and agricultural fields in the south, probably due to the availability of habitats in different parts of Iberia.
The Effect of Fly Ash Additive on the Thermal Conductivity of Polystyrene Concrete
Authors: Tlegenov Rassul B. , Niyazbekova Rimma K. , Jexembayeva Assel E. , Korniejenko Kinga , Aruova Lyazat B. , Aldabergenova Saule S. , Maykonov Aslan S.
Published: September 2024

The use of fly ash in compositions as a substitute for a part of cement is economically favorable and ecologically feasible in connection with large accumulations of waste at the enterprises of the energy sector. In addition, the technology of cement production provides high-temperature treatment of mineral substances in kilns with significant emissions of carbon dioxide. One of the most effective directions of the utilization of fly ash is their use in concrete composites. The use of this material will provide the required temperature and humidity conditions in residential premises, solve the problem of “cold bridges” in structures, minimize heat losses of the structure, and increase the energy efficiency of buildings in general. At the same time, polystyrene concrete, due to its structural structure and the presence of thermally conductive concrete, has limited opportunities for thermal and physical–mechanical properties. To improve the operational properties of polystyrene concrete, it is proposed to use composite binders, including fly ash from the thermal power station of Astana. The main aim of this study is to develop compositions of polystyrene concrete with reduced thermal conductivity and improved physical and mechanical properties. The objectives of this study include the determination of characteristics of fly ash from Astana, formulation of polystyrene concrete mixtures with different proportions of fly ash, and evaluation of their thermal conductivity properties. These tasks are in line with the objectives of the ISO 50001 standard to improve energy efficiency and reduce environmental impact. The results showed that the addition of fly ash from Astana to polystyrene concrete leads to a marked reduction in thermal conductivity, contributing to improved energy efficiency of the building envelope. Optimal results were achieved by using 15% of Astana fly ash as an additive in polystyrene concrete, which led to a significant reduction in thermal conductivity of 51.47%. This reduction is in line with improving the energy efficiency of building materials, especially in cold climates.
Study on the Shear Performance of the Interface between Post-Cast Epoxy Resin Concrete and Ordinary Concrete
Authors: Chen Peiqi , Wang Hao , Zhou Xiaojie , Zhao Shilong
Published: September 2024

The interface of fresh-aged concrete represents a critical vulnerability within monolithic assembled monolithic concrete structures. In this paper, the shear performance of the interface between post-cast epoxy resin concrete and standard concrete is studied using experimental methods and finite element analysis. The objective is to furnish empirical data that support the broader adoption of epoxy resin concrete in assembled structures. A direct shear experiment of 19 Z-shaped samples and a computation of 20 finite element models were completed. The results from both experimental and computational analyses provided insights into several factors influencing the shear performance at the interface. These factors include the pre-cast part of concrete strength, the friction coefficient of the interface, the longitudinal reinforcement ratio at the interface, the compressive strength of concrete in the post-cast part, and confining stress. The findings indicate that utilizing epoxy resin concrete for post-cast material, roughing the interface, and setting keyways can enhance the shear performance of the interface so that it equals or even exceeds the cast-in situ sample. Optimal shear results are obtained when the compressive strength of the post-cast epoxy resin concrete closely matches that of the pre-cast conventional cement. Moreover, increasing the depth of the keyways rather than their width is more effective in improving the shear capacity of the sample. It is recommended that the depth of the keyway should be at least 30 mm, and its width should be no less than three times the depth. As the longitudinal reinforcement ratio at the interface increases, there is an enhancement in shear capacity coupled with a reduction in deformative performance. It is advisable to maintain this ratio below 1.0% to balance the strength and ductility effectively.
Numerical and Theoretical Study on Flexural Performance and Reasonable Structural Parameters of New Steel Grating–UHPFRC Composite Bridge Deck in Negative Moment Zone
Authors: Ma Jianyong , Yuan Haoyun , Zhang Jiahao , Luo Zuolong
Published: September 2024

As the bridge’s structural component is directly subjected to vehicle loads, the stress performance of the bridge deck has a significant impact on the safety, durability, and driving comfort of the bridge. In order to improve the bending performance of the bridge deck in the negative moment zone, a new type of steel grating–UHPFRC composite bridge deck was proposed in this paper. Firstly, structural details and advantages of the new steel grating-UHPFRC composite bridge deck were introduced. Secondly, the finite element program ABAQUS was used to establish a refined solid finite element model of the new bridge deck. The mathematical program MATLAB (PYTHON) was also used to analyze the effects of the structural parameters on bending bearing capacity and put forward reasonable structural parameters of the new bridge deck, considering the technical and economic indexes. Thirdly, the simplified plasticity theory was applied to analyze the bending bearing capacity of the new bridge deck, and the corresponding formula for bending bearing capacity calculation was derived and verified by numerical model results. In addition, the cost–benefit analysis and environmental impact assessment of the new bridge deck were also conducted. The results show that the bending bearing capacity of the new bridge deck in the negative moment zone increases with the increase of the width of the bridge deck, the thickness of the wing plate, and the height of the web plate, with a trend of increasing and then decreasing when the horizontal inclination of the web plate decreases. The bridge deck width does not have a significant effect on improving the bearing capacity. The bearing capacity calculated by theoretical formulas is close to that calculated by numerical models and the maximum relative deviation is 9.1%. The new steel grating-UHPFRC composite bridge deck proposed in this paper is superior to conventional steel-UHPC composite bridge deck in terms of cost-benefit and environmental impact.
The Role of Pulmonary Collectins, Surfactant Protein A (SP-A) and Surfactant Protein D (SP-D) in Cancer
Authors: Cedzyński Maciej , Świerzko Anna S.
Published: September 2024

Surfactant proteins A and D (SP-A and SP-D) belong to the collectin subfamily of C-type oligomeric lectins. They are pattern-recognition molecules (PRMs), able to recognise pathogen- or danger-associated molecular patterns (PAMPs, DAMPs) in the presence of Ca2+ cations. That property enables opsonisation or agglutination of non-self or altered/abnormal self cells and contributes to their clearance. Like other collectins, SP-A and SP-D are characterised by the presence of four distinct domains: a cysteine-rich domain (at the N-terminus), a collagen-like region, an α-helical neck domain and a globular carbohydrate-recognition domain (CRD) (at the C-terminus). Pulmonary surfactant is a lipoprotein complex, preventing alveolar collapse by reducing surface tension at the air–liquid interface. SP-A and SP-D, produced by type II alveolar epithelial cells and Clara cells, are not only pattern-recognition molecules but also contribute to the surfactant structure and homeostasis. Moreover, they are expressed in a variety of extrapulmonary sites where they are involved in local immunity. The term “cancer” includes a variety of diseases: tumours start from uncontrolled growth of abnormal cells in any tissue which may further spread to other sites of the body. Many cancers are incurable, difficult to diagnose and often fatal. This short review summarises anti- and pro-tumorigenic associations of SP-A and SP-D as well as perspectives of their usefulness in cancer diagnosis and therapy.
Conjunctival Melanoma: A Clinical Review and Update
Authors: Butt Karam , Hussain Rumana , Coupland Sarah , Krishna Yamini
Published: September 2024

Conjunctival melanoma (Co-M) is an aggressive, invasive eye and eyelid cancer. Its global incidence of ~1 in a million is increasing at a rate ratio of ~1.4, but this rises sharply in over 65-year-olds. Although rare, Co-M has a devastating impact on the lives of those who develop it. Co-M is often misdiagnosed or overlooked, leading to vision loss either from the destructive effects of the tumour or side effects of therapy, facial disfigurement from radical surgery, and death from metastases. Due to its rarity, there is limited evidence for diagnosis and management; hence, there is no standardised treatment and not all cases are referred to a specialised ocular oncology centre. Recent progress in cancer immunology and genetics have revolutionised the treatment of cutaneous melanomas, which share some similarities to Co-M. Importantly, a better understanding of Co-M and its precursor lesions is urgently needed to lead to the development of novel targeted and immunotherapies both for local tumour control and disseminated disease. This review aims to provide a comprehensive clinical overview of the current knowledge regarding Co-M, its epidemiology, pathogenesis, presentation, diagnosis and recent changes in the classification of its precursor lesions, management, and recent advances in novel biological therapies for personalised treatment of this disease.
FGF23 and Cell Stress in SaOS-2 Cells—A Model Reflecting X-Linked Hypophosphatemia Dynamics
Authors: Brueck Lisanne , Roocke Sascha , Matschke Veronika , Richter-Unruh Annette , Marcus-Alic Katrin , Theiss Carsten , Stahlke Sarah
Published: September 2024

Our study investigates the impact of FGF23 overexpression on SaOS-2 cells to elucidate its role in cellular stress and morphology, contributing to the understanding of skeletal pathologies like X-linked hypophosphatemia (XLH). Using transmission electron microscopy and protein analysis (Western blot), we analyzed the rough endoplasmic reticulum (rER) and mitochondria in SaOS-2 cells with FGF23 overexpression compared to controls. We found significant morphological changes, including enlarged and elongated rER and mitochondria, with increased contact zones, suggesting enhanced interaction and adaptation to elevated protein synthesis and secretion demands. Additionally, we observed higher apoptosis rates of the cells after 24–72 h in vitro and upregulated proteins associated with ER stress and apoptosis, such as CHOP, XBP1 (spliced and unspliced), GRP94, eIF2α, and BAX. These findings indicate a robust activation of the unfolded protein response (UPR) and apoptotic pathways due to FGF23 overexpression. Our results highlight the critical role of ER and mitochondrial interactions in cellular stress responses and provide new insights into the mechanistic link between FGF23 signaling and cellular homeostasis. In conclusion, our study underscores the importance of analyzing UPR-related pathways in the development of therapeutic strategies for skeletal and systemic diseases and contributes to a broader understanding of diseases like XLH.
The Oncoprotein Fra-2 Drives the Activation of Human Endogenous Retrovirus Env Expression in Adult T-Cell Leukemia/Lymphoma (ATLL) Patients
Authors: Tram Julie , Marty Laetitia , Mourouvin Célima , Abrantes Magali , Jaafari Ilham , Césaire Raymond , Hélias Philippe , Barbeau Benoit , Mesnard Jean-Michel , Baccini Véronique , Chaloin Laurent , Peloponese Jean-Marie Jr.
Published: September 2024

Human endogenous retroviruses (HERVs) are retroviral sequences integrated into 8% of the human genome resulting from ancient exogenous retroviral infections. Unlike endogenous retroviruses of other mammalian species, HERVs are mostly replication and retro-transposition defective, and their transcription is strictly regulated by epigenetic mechanisms in normal cells. A significant addition to the growing body of research reveals that HERVs’ aberrant activation is often associated with offsetting diseases like autoimmunity, neurodegenerative diseases, cancers, and chemoresistance. Adult T-cell leukemia/lymphoma (ATLL) is a very aggressive and chemoresistant leukemia caused by the human T-cell leukemia virus type 1 (HTLV-1). The prognosis of ATLL remains poor despite several new agents being approved in the last few years. In the present study, we compare the expression of HERV genes in CD8+-depleted PBMCs from HTLV-1 asymptomatic carriers and patients with acute ATLL. Herein, we show that HERVs are highly upregulated in acute ATLL. Our results further demonstrate that the oncoprotein Fra-2 binds the LTR region and activates the transcription of several HERV families, including HERV-H and HERV-K families. This raises the exciting possibility that upregulated HERV expression could be a key factor in ATLL development and the observed chemoresistance, potentially leading to new therapeutic strategies and significantly impacting the field of oncology and virology.
Impact of Diamond-like Carbon Films on Reverse Torque: Superior Performance in Implant Abutments with Internal Conical Connections
Authors: Coelho Arianne Vallim Pinto , Figueiredo Viviane Maria Gonçalves de , Ferreira Leandro Lameirão , Silva Alecsandro de Moura , Oliani Marcelo Gallo , Queiroz José Renato Cavalcanti de , Sobrinho Argemiro Soares da Silva , Nogueira Junior Lafayette , Prado Renata Falchete do
Published: September 2024

The loosening or fracture of the prosthetic abutment screw is the most frequently reported complication in implant dentistry. Thin diamond-like carbon (DLC) films offer a low friction coefficient and high wear resistance, functioning as a solid lubricant to prevent the weakening of the implant–abutment system. This study evaluated the effects of DLC nanofilms on the reverse torque of prosthetic abutments after simulated chewing. Abutments with 8° and 11° taper connections, with and without DLC or silver-doped DLC coatings, were tested. The films were deposited through the plasma enhanced chemical vapor deposition process. After two million cycles of mechanical loading, reverse torque was measured. Analyses with scanning electron microscopy were conducted on three samples of each group before and after mechanical cycling to verify the adaptation of the abutments. Tribology, Raman and energy-dispersive spectroscopy analyses were performed. All groups showed a reduction in insertion torque, except the DLC-coated 8° abutments, which demonstrated increased reverse torque. The 11° taper groups experienced the most torque loss. The nanofilm had no significant effect on maintaining insertion torque, except for the DLC8 group, which showed improved performance.
Trust Us—We Are the (COVID-19 Misinformation) Experts: A Critical Scoping Review of Expert Meanings of “Misinformation” in the Covid Era
Authors: Chaufan Claudia , Hemsing Natalie , Heredia Camila , McDonald Jennifer
Published: September 2024

Since the WHO declared COVID-19 a pandemic, prominent social actors and institutions have warned about the threat of misinformation, calling for policy action to address it. However, neither the premises underlying expert claims nor the standards to separate truth from falsehood have been appraised. We conducted a scoping review of the medical and social scientific literature, informed by a critical policy analysis approach, examining what this literature means by misinformation. We searched academic databases and refereed publications, selecting a total of 68 articles for review. Two researchers independently charted the data. Our most salient finding was that verifiability relied largely on the claims of epistemic authorities, albeit only those vetted by the establishment, to the exclusion of independent evidentiary standards or heterodox perspectives. Further, “epistemic authority” did not depend necessarily on subject matter expertise, but largely on a new type of “expertise”: in misinformation itself. Finally, policy solutions to the alleged threat that misinformation poses to democracy and human rights called for suppressing unverified information and debate unmanaged by establishment approved experts, in the name of protecting democracy and rights, contrary to democratic practice and respect for human rights. Notably, we identified no pockets of resistance to these dominant meanings and uses. We assessed the implications of our findings for democratic public policy, and for fundamental rights and freedoms.
Microwave-Assisted Fabrication and Characterization of Carbon Fiber-Sodium Bismuth Titanate Composites
Authors: Azam Fareeha , Rafiq Muhammad Asif , Ahmed Furqan , Moqbool Adnan , Fayyaz Osama , Imran Zerfishan , Habib Muhammad Salman , Shakoor Rana Abdul
Published: September 2024

Lead-based piezoelectric materials cause many environmental problems, regardless of their exceptional performance. To overcome this issue, a lead-free piezoelectric composite material was developed by incorporating different percentages of carbon fiber (CF) into the ceramic matrix of Bismuth Sodium Titanate (BNT) by employing the microwave sintering technique. The aim of this study was also to evaluate the impact of microwave sintering on the microstructure and the electrical behavior of the carbon-fiber-reinforced Bi0.5Na0.5TiO3 composite (BNT-CF). A uniform distribution of the CF and increased densification of the BNT-CF was achieved, leading to improved piezoelectric properties. X-ray diffraction (XRD) showed the formation of a phase-pure crystalline perovskite structure consisting of CF and BNT. A Field Emission Scanning electron microscope (FESEM) revealed that utilizing microwave sintering at lower temperatures and shorter dwell times results in a superior densification of the BNT-CF. Raman Spectroscopy confirmed the perovskite structure of the BNT-CF and the presence of a Morphotropic Phase Boundary (MPB). An analysis of nanohardness indicated that the hardness of the BNT-CF increases with the increasing amount of CF. It is also revealed that the electrical conductivity of the BNT-CF at a low frequency is significantly influenced by the amount of CF and the temperature. Moreover, an increase in the carbon fiber concentration resulted in a decrease in dielectric properties. Finally, a lead-free piezoelectric BNT-CF showing dense and uniform microstructure was developed by the microwave sintering process. The promising properties of the BNT-CF make it attractive for many industrial applications.
Taxonomic Exploration of Rare Amphipods: A New Genus and Two New Species (Amphipoda, Iphimedioidea, Laphystiopsidae) Described from Seamounts in the Western Pacific
Authors: Wang Yanrong , Sha Zhongli , Ren Xianqiu
Published: September 2024

During two expeditions to the seamounts in the Yap-Caroline area of the Western Pacific, a new genus, Phoxirostus gen. nov., in the family Laphystiopsidae Stebbing, 1899, is erected for two new species, P. longicarpus sp. nov. (type species) and P. yapensis sp. nov. The new genus can be distinguished from the other three laphystiopsid genera by the acute rostrum not overreaching the distal end of the first peduncular article of antenna 1, the outer plate of maxilla 1 bearing 10–11 spines, and the elongated carpus of pereopods 3–7 being distinctly longer than half the length of the propodus. Phoxirostus longicarpus sp. nov. differs from P. yapensis sp. nov. by the shape of the eyes and coxa 4, the presence of posterodistal protrusions on pleonite 1, and the number of posterodistal protrusions on pleonite 2. Generic analysis of one mitochondrial (COI) and one nuclear (H3) gene using maximum likelihood and Bayesian inference clarified the phylogenetic position of the Laphystiopsidae within the superfamily Iphimedioidea Boeck, 1871.
Evaluation of the Allplex GI Parasite and Helminth PCR Assay in a Belgian Travel Clinic
Authors: Coppens Jasmine , Drieghe Charlotte , Potters Idzi , Schwob Jean-Marc , Van Esbroeck Marjan
Published: September 2024

Recently a number of broad-range stool parasite PCR assays have been developed. However, there is ongoing disagreement regarding their diagnostic performance, as various studies have produced contradictory results. In this study, we compared the diagnostic accuracy of the Seegene Allplex GI-Parasite and Allplex GI-Helminth assays (SA) with the conventional methods used at the travel clinic of the Institute of Tropical Medicine (ITM) including microscopy, antigen testing, and molecular detection in order to provide insights into the strengths and limitations of this diagnostic tool which may be crucial to select the most appropriate diagnostic tools for the suspected pathogen. A total of 97 native stool samples from 95 patients with suspected gastrointestinal illness were analyzed, including 26 from a frozen collection and 71 prospectively collected samples. The diagnostic performance of SA was notably superior to the conventional workflow in detecting Dientamoeba fragilis (sensitivity 100% vs. 47.4%) and Blastocystis hominis (sensitivity 95% vs. 77.5%). SA had a comparable performance with the conventional workflow in detecting pathogenic protozoa (sensitivity 90% vs. 95%). In contrast, SA had a much lower diagnostic performance in detecting helminths (59.1%) compared to the conventional workflow (100%). We conclude that the Seegene Allplex GI-Parasite assay may be useful for protozoa screening in low-endemic industrialized countries. However, the Allplex GI-Helminth assay is not recommended due to its suboptimal performance compared to microscopy.
Evaluating Prediction Models with Hearing Handicap Inventory for the Elderly in Chronic Otitis Media Patients
Authors: Yoon Hee Soo , Kim Min Jin , Lim Kang Hyeon , Kim Min Suk , Kang Byung Jae , Rah Yoon Chan , Choi June
Published: September 2024

Background: This retrospective, cross-sectional study aimed to assess the functional hearing capacity of individuals with Chronic Otitis Media (COM) using prediction modeling techniques and the Hearing Handicap Inventory for the Elderly (HHIE) questionnaire. This study investigated the potential of predictive models to identify hearing levels in patients with COM. Methods: We comprehensively examined 289 individuals diagnosed with COM, of whom 136 reported tinnitus and 143 did not. This study involved a detailed analysis of various patient characteristics and HHIE questionnaire results. Logistic and Random Forest models were employed and compared based on key performance metrics. Results: The logistic model demonstrated a slightly higher accuracy (73.56%), area under the curve (AUC; 0.73), Kappa value (0.45), and F1 score (0.78) than the Random Forest model. These findings suggest the superior predictive performance of the logistic model in identifying hearing levels in patients with COM. Conclusions: Although the AUC for the logistic regression did not meet the benchmark, this study highlights the potential for enhanced reliability and improved performance metrics using a larger dataset. The integration of prediction modeling techniques and the HHIE questionnaire shows promise for achieving greater diagnostic accuracy and refining intervention strategies for individuals with COM.
Utility of Ultrasound-Guided Attenuation Parameter (UGAP) in Renal Angiomyolipoma (AML): First Results
Authors: Kranert Paul Christian , Kranert Paula , Banas Miriam C. , Jung Ernst Michael , Banas Bernhard , Putz Franz Josef
Published: September 2024

Angiomyolipoma (AML) are the most common benign solid renal mass. Differentiation from malignant tumours is essential. Imaging features in ultrasound may overlap between malignant lesions, especially between renal cell carcinoma (RCC) and AML. So far, sectional imaging has been necessary for reliable differentiation. The aim of this study is to evaluate the use of the ultrasound-guided attenuation parameter (UGAP), a recently established tool for assessing hepatic steatosis, in the differentiation of AMLs from other renal masses. Therefore, 27 patients with unknown solid renal masses were examined by ultrasound including UGAP. The attenuation was assessed qualitatively by attenuation map and quantitatively in comparison to the surrounding renal tissue. UGAP was applicable in 26/27 patients. Findings were compared with CT/MRI as the current imaging standard. A total of 18 AML and 9 other renal tumours were found. The diagnostic performance of B-Mode (hyperechogenic lesion) ultrasound was 77.8% in identifying AML. The diagnostic performance of the attenuation map showed a diagnostic performance of 92.6%, whereby UGAP measurements were successful in 76.9% of cases. Quantitatively, we found a significant difference (p < 0.034) in mean measured attenuation between AML (0.764 ± 0.162 dB/cm/MHz) vs. other renal tumours (0.658 ± 0.155 dB/cm/MHz). The best performance was found by a combined parameter of a hyperechogenic lesion with a positive attenuation map with an accuracy of 95.0%. In conclusion, UGAP may represent a possibility for differentiating solid renal lesions more accurately by ultrasound, especially classic hyperechoic AMLs from other renal lesions. Further studies are needed to increase the diagnostic reliability further.
Concentration Scales and Solvation Thermodynamics: Some Theoretical and Experimental Observations Regarding Spontaneity and the Partition Ratio
Authors: Silva Diego J. Raposo da , Souza Jéssica I. R. de , Longo Ricardo L.
Published: September 2024

The solvation thermodynamics (ST) formalism proposed by A. Ben-Naim is a mathematically rigorous and physically grounded theory for describing properties related to solvation. It considers the solvation process as the transfer of a molecule (“solute”) from a fixed position in the ideal gas phase to a fixed position within the solution. Thus, it removes any contribution to the solvation process that is not related to the interactions between this molecule and its environment in the solution. Because ST is based on statistical thermodynamics, the natural variable is number density, which leads to the amount (or “molar”) concentration scale. However, this choice of concentration scale is not unique in classical thermodynamics and the solvation properties can be different for commonly used concentration scales. We proposed and performed experiments with diethylamine in a water/hexadecane heterogeneous mixture to confront the predictions of the ST, based on the amount (or “molar”) concentration scale, and the Fowler–Guggenheim formalism, based on the mole fraction scale. By means of simple acid–base titration and 1H NMR measurements, it was established that the predictions of differences in the solvation Gibbs energy and the partition ratio (or “partition coefficient”) of diethylamine between water and hexadecane are consistent with the ST formalism. Additionally, with current literature data, we have shown additional experimental support for the ST. However, due to the arbitrariness of the relative amount of solvents in the partition ratio, the choice of a single concentration scale within the classical thermodynamics is still not possible.
A Statistical Analysis of Fluid Interface Fluctuations: Exploring the Role of Viscosity Ratio
Authors: Heijkoop Selwin , Rieder David , Moura Marcel , Rücker Maja , Spurin Catherine
Published: September 2024

Understanding multiphase flow through porous media is integral to geologic carbon storage or hydrogen storage. The current modelling framework assumes each fluid present in the subsurface flows in its own continuously connected pathway. The restriction in flow caused by the presence of another fluid is modelled using relative permeability functions. However, dynamic fluid interfaces have been observed in experimental data, and these are not accounted for in relative permeability functions. In this work, we explore the occurrence of fluid fluctuations in the context of sizes, locations, and frequencies by altering the viscosity ratio for two-phase flow. We see that the fluctuations alter the connectivity of the fluid phases, which, in turn, influences the relative permeability of the fluid phases present.
Distance Decay of Urban Park Visitation: Roles of Personal Characteristics and Visitation Patterns
Authors: Shu Di , Peng Yulin , Zhang Ziyu , Shi Ruirui , Wu Can , Gan Dexin , Li Xiaoma
Published: September 2024

Distance decay of urban park visitation (e.g., visitation number and visitation frequency) has been widely acknowledged and is increasingly integrated into urban park planning and management considering spatial accessibility and service equity. However, thorough understandings especially concerning the variations among visitors with different personal characteristics and visitation patterns are still scarce. Taking Changsha, China as an example, we collected data on visitation distance (i.e., the distance between urban parks and visitor’s homes) and visitation frequency of 2535 urban park visitors, modeled the distance decay of visitation density and visitation frequency, and investigated their variations among visitors with different personal characteristics and visitation patterns. The results show that: (1) The median visitation distance was 1.3 km and the median visitation frequency was 24 times per season. (2) Both visitation density and visitation frequency showed clear spatial patterns of distance decay and can be effectively modeled using common distance decay functions (e.g., power function, exponential function, and logarithmic function). (3) Visitors’ characteristics (e.g., gender and age) and visitation patterns (e.g., duration time, transportation modes, and visitation purposes) significantly impact visitation distance, visitation frequency, and the characteristics of distance decay (i.e., the rate of distance decay). These findings extend our understanding of the distance decay of urban park visitation which can help better urban park planning and management.
Conservation and Avoided Deforestation: Evidence from Protected Areas of Tanzania
Authors: Gizachew Belachew , Shirima Deo D. , Rizzi Jonathan , Kukunda Collins B. , Zahabu Eliakimu
Published: September 2024

Tanzania dedicates a substantial proportion (38%) of its territory to conservation, with a large number of Protected Areas (PAs) managed under various regimes. Nevertheless, the country still experiences high rates of deforestation, which threaten the ecological integrity and socio-economic benefits of its forests. We utilized the Global Forest Change Dataset (2012–2022) and implemented a Propensity Score Matching (PSM) approach followed by a series of binomial logit regression modeling. Our objectives were to evaluate (1) the likelihood of PAs in avoiding deforestation compared with unprotected forest landscapes, (2) the variability in effectiveness among the different PA management regimes in avoiding deforestation, (3) evidence of leakage, defined here as the displacement of deforestation beyond PA boundaries as a result of protection inside PAs. Our findings reveal that, despite ongoing deforestation within and outside of PAs, conservation efforts are, on average, three times more likely to avoid deforestation compared with unprotected landscapes. However, the effectiveness of avoiding deforestation significantly varies among the different management regimes. National Parks and Game Reserves are nearly ten times more successful in avoiding deforestation, likely because of the stringent set of regulations and availability of resources for implementation. Conversely, Nature Forest Reserves, Game Controlled Areas, and Forest Reserves are, on average, only twice as likely to avoid deforestation, indicating substantial room for improvement. We found little evidence of the overall leakage as a consequence of protection. These results highlight the mixed success of Tanzania’s conservation efforts, suggesting opportunities to enhance the effectiveness of many less protected PAs. We conclude by proposing potential strategic pathways to enhance further the climate and ecosystem benefits of conservation in Tanzania.
Computational Fluid Dynamics Study on Bottom-Hole Multiphase Flow Fields Formed by Polycrystalline Diamond Compact Drill Bits in Foam Drilling
Authors: Wei Lihong , Honra Jaime
Published: September 2024

High-temperature geothermal wells frequently employ foam drilling fluids and Polycrystalline Diamond Compact (PDC) drill bits. Understanding the bottom-hole flow field of PDC drill bits in foam drilling is essential for accurately analyzing their hydraulic structure design. Based on computational fluid dynamics (CFD) and multiphase flow theory, this paper establishes a numerical simulation technique for gas-liquid-solid multiphase flow in foam drilling with PDC drill bits, combined with a qualitative and quantitative hydraulic structure evaluation method. This method is applied to simulate the bottom-hole flow field of a six-blade PDC drill bit. The results show that the flow velocity of the air phase in foam drilling fluid is generally higher than that of the water phase. Some blades’ cutting teeth exhibit poor cleaning and cooling effects, with individual cutting teeth showing signs of erosion damage and cuttings cross-flow between channels. To address these issues, optimizing the nozzle spray angle and channel design is necessary to improve hydraulic energy distribution, enhance drilling efficiency, and extend drill bit life. This study provides new ideas and methods for developing geothermal drilling technology in the numerical simulation of a gas-liquid-solid three-phase flow field. Additionally, the combined qualitative and quantitative evaluation method offers new insights and approaches for research and practice in drilling engineering.