bluuebunny/crossref_metadata_2025 huggingface dataset:
The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles
Authors: Dallastella Marianna , Oliveira Willian Klassen de , Rodrigues Marcio L. , Goldenberg Samuel , Alves Lysangela R.
Published: September 2023

RNA-binding proteins (RBPs) are essential for regulating RNA metabolism, stability, and translation within cells. Recent studies have shown that RBPs are not restricted to intracellular functions and can be found in extracellular vesicles (EVs) in different mammalian cells. EVs released by fungi contain a variety of proteins involved in RNA metabolism. These include RNA helicases, which play essential roles in RNA synthesis, folding, and degradation. Aminoacyl-tRNA synthetases, responsible for acetylating tRNA molecules, are also enriched in EVs, suggesting a possible link between these enzymes and tRNA fragments detected in EVs. Proteins with canonical RNA-binding domains interact with proteins and RNA, such as the RNA Recognition Motif (RRM), Zinc finger, and hnRNP K-homology (KH) domains. Polyadenylate-binding protein (PABP) plays a critical role in the regulation of gene expression by binding the poly(A) tail of messenger RNA (mRNA) and facilitating its translation, stability, and localization, making it a key factor in post-transcriptional control of gene expression. The presence of proteins related to the RNA life cycle in EVs from different fungal species suggests a conserved mechanism of EV cargo packing. Various models have been proposed for selecting RNA molecules for release into EVs. Still, the actual loading processes are unknown, and further molecular characterization of these proteins may provide insight into the mechanism of RNA sorting into EVs. This work reviews the current knowledge of RBPs and proteins related to RNA metabolism in EVs derived from distinct fungi species, and presents an analysis of proteomic datasets through GO term and orthology analysis, Our investigation identified orthologous proteins in fungal EVs on different fungal species.
Left bundle branch pacing preserved left ventricular myocardial work in patients with bradycardia
Authors: Chen Huang-Chung , Liu Wen-Hao , Chen Yung-Lung , Lee Wei-Chieh , Fang Yen-Nan , Chong Shaur-Zheng , Chen Mien-Cheng
Published: September 2023

Background

Left bundle branch pacing (LBBP) is an emerging physiological pacing modality. Left ventricular (LV) myocardial work (MW) incorporates afterload and LV global longitudinal strain to estimate global and segmental myocardial contractility. However, the effect of LBBP on LV MW remains unknown. This study aimed to evaluate the impact of LBBP on LV MW in patients receiving pacemaker for bradyarrhythmia.

Methods

We prospectively enrolled 70 bradycardia patients with normal LV systolic function receiving LBBP (n = 46) and non-selective His-bundle pacing (NS-HBP) (n = 24). For comparative analysis, patients receiving right ventricular pacing (RVP) (n = 16) and control subjects (n = 10) were enrolled. Two-dimensional speckle tracking echocardiography was performed. The LV pressure-strain loop was non-invasively constructed to assess global LV MW.

Results

After 6-month follow-up, LBBP group (with >40% ventricular pacing during 6 months) had shorter peak strain dispersion (PSD) compared with RVP group, and higher LV global longitudinal strain compared with RVP group and NS-HBP group, but had no difference in left intraventricular mechanical dyssynchrony, including septal-to-posterior wall motion delay and PSD, compared with NS-HBP group. During ventricular pacing, LBBP group had higher global MW index (GWI) (2,189 ± 527 vs. 1,493 ± 799 mmHg%, P = 0.002), higher global constructive work (GCW) (2,921 ± 771 vs. 2,203 ± 866 mmHg%, P = 0.009), lower global wasted work (GWW) (211 ± 161 vs. 484 ± 281 mmHg%, P < 0.001) and higher global MW efficiency (GWE) (91.4 ± 5.0 vs. 80.9 ± 8.3%, P < 0.001) compared with RVP group, and had lower GWW (211 ± 161 vs. 406 ± 234 mmHg%, P < 0.001) and higher GWE (91.4 ± 5.0 vs. 86.4 ± 8.1%, P < 0.001) compared with NS-HBP group.

Conclusions

In this study we found that in patients with mid-term (6-month) high ventricular pacing burden (>40%), LBBP preserved more LV MW compared with NS-HBP and RVP. Further studies are warranted to assess the association between LV MW and long-term clinical outcomes in LBBP with high ventricular pacing burden.
“A land whose stones are iron...”—Iron ore sources in the Southern Levant
Authors: Eliyahu-Behar Adi , Ben Dor Yoav , Brauns Michael , Shulman Oriana Adi , Avni Yoav , Killebrew Ann E. , Tirosh Ofir , Yahalom-Mack Naama
Published: September 2023

Recent discoveries of iron production remains in multiple Iron Age sites throughout the Southern Levant indicate the significance of iron production in the region during this time. However, one main question remains unanswered—that is, provenance: Where were the iron resources located? Were they local or brought from afar? Answering these questions would allow great potential for reconstructing iron-ore procurement strategies and exploitation practices in the social, political, and economic context of the Southern Levant in the early 1st millennium BCE. It has long been assumed that iron ore, prevalent in the Earth's crust, is widely available. However, since high-grade ore was required for the smelting technology of the time, the bloomery process, only selected iron ore deposits in the region were suitable. This study aims to identify and characterize chemically and isotopically enriched iron-ore sources in the Southern Levant. Samples were collected from sedimentary and hydrothermal sources and analyzed for their iron content, trace elements composition, and osmium (Os) isotopic ratios. Here, we present the results of this preliminary investigation, introducing several substantial ore sources in the region and demonstrating the possibility of differentiating between these sources based on their chemical and Os isotopic composition. Finally, hematite objects from selected Iron Age archaeological sites are compared against the analyzed ore sources, showing inconsistency with the sampled ores.
Changes in land use and ecosystem service value in desert areas of China after reform and opening up
Authors: Ba Zhidan , Du Huishi , Hasi Eerdun , Lu Xiaoling
Published: September 2023

Desert areas of China have important ecological functions, so analyzing changing characteristics of ecosystem service values in this region is important for sustainable development. Using land use change data for desert areas from 1978 to 2022, this paper combined the equivalent factor method and the actual situation of the study area to revise value coefficients of ecosystem services and study characteristics of land use and ecosystem service value changes after reform and opening up. The results showed that after reform and opening up, the area of plow, water, and other lands in the study area decreased, while grassland, forest, construction land, and sandy land increased. The conversion of a land use type mainly occurred as conversion of grassland and other lands to other land use types. The value of ecosystem services increased, increasing by 19.63 × 108 CNY. From the perspective of land use type in 2022, the ecosystem service value of grassland was the highest, reaching 12.19 × 108 CNY, an increase of 11.73 × 108 CNY compared with the early stage of reform and opening up. From the perspective of ecosystem service types in 2022, the value of maintaining soil ecological services was the highest, reaching 3.07 × 108 CNY, an increase of 2.97 × 108 CNY compared with the beginning of reform and opening up. From the perspective of the ecological sensitivity index, the sensitivity index results for the ecosystem service value in the study area were all <1, and the research results were credible. From the perspective of the land ecological coordination degree, the overall land ecological coordination degree in the study area was at a moderate coordination level, indicating that land use change did not lead to environmental deterioration, but there was a crisis. Therefore, increasing and maintaining ecological land is the key to improving ESV in the study area.
Key technologies research on of soil-structure interaction base story isolated structure response in 3D seismic zone
Authors: Xu Jingran , Gao Haolun , Guo Zihan , Zhao Jie , Yang Zhuoxin , Zhao Guangxing , Guo Zhongfa , Liu Dewen , Sun Weiwei
Published: September 2023

The development of karst in Karst area leads to poor stability of stratum. If earthquake occurs, the area will produce destructive disaster. In order to improve the stability capacity of the grassroots in the region, this study investigates the seismic response of inter-story isolation structures considering soil-structure interaction (SSI) in three-dimensional earthquakes. A model of the inter-story isolation structure incorporating SSI was developed, and one-dimensional, two-dimensional, and three-dimensional ground motions were applied to compare the seismic response under different input conditions. A three-dimensional isolation system was introduced and compared with traditional horizontal isolation structures to address excessive tensile and compressive stresses on the isolation structure during three-dimensional ground motion. The results demonstrate that the seismic response to three-dimensional earthquakes surpasses one-dimensional and two-dimensional inputs. Furthermore, adding a three-dimensional isolation structure effectively isolates vertical ground motion and reduces structural seismic response. Moreover, it minimizes soil stresses on the foundation compared to traditional horizontal isolation structure, enhancing foundation stability. This study will provide theoretical value and practical guidance for the research on key technology of SSI base story isolation structure response in Karst Plateau 3D Seismic zone.
The prevalence of careless response behaviour and its consequences on data quality in self-report questionnaires on student learning
Authors: Chauliac Margot , Willems Jonas , Gijbels David , Donche Vincent
Published: September 2023

Introduction

Self-report questionnaires are widely used in high schools and universities to gain insights into students’ learning strategies and enhance the quality of their education. However, it is important to acknowledge the possibility of respondents being inattentive when completing these questionnaires. While reliability analyses are typically performed at the group level, when providing individual feedback, it is crucial that each respondent’s results are reliable. This study aimed to evaluate the prevalence of careless response behaviour in a questionnaire concerning student learning.

Methods

Data analysis encompassed a substantial sample of 12,000+ students in their final two years of secondary education, averaging around 17 years of age. Employing five complementary detection techniques, the study identified instances of careless responding present in the questionnaire data.

Results

Our results underscore a notable prevalence of careless response behaviour among the surveyed students. Application of the five detection techniques revealed a substantial number of instances indicating inattentive responding. Furthermore, the questionnaire’s measurement scales were evaluated for reliability. The study noted the presence of carelessness but observed minimal impact on group-level results.

Discussion

The outcomes of this study hold important implications for using self-report questionnaires in education. The prevalence of careless responding emphasizes the need for scrutinizing individual responses. Despite careless responses, their influence on overall group-level data integrity seems restricted. Nonetheless, the study underscores the importance of cautiously interpreting individual-level outcomes, particularly when using these results for individual feedback.
School inspections: a rhetoric of quality or reality?
Authors: Gardezi Sarah , McNamara Gerry , Brown Martin , O’Hara Joe
Published: September 2023

This study aims to explore the perspectives of school inspectors and leaders on the quality of school inspection in four countries with different inspection systems: Dubai, Ireland, New Zealand, and Pakistan. The study also examines the general perceptions of school leaders and inspectors about the impact of quality assurance agencies on teaching and learning in these countries. Using semi-structured interviews of school leaders (n = 28) and inspectors (n = 14), the research found that school leaders’ experiences and perceptions of school inspection and evaluation were varied in and across all four countries. While some expressed dissatisfaction with the inspectors and the process, others regarded it as a beneficial endeavor that instilled a sense of vigilance and attentiveness toward the quality standards and inspection criteria. School leaders’ experiences and perceptions were mostly positive in Dubai and mixed in the cases of Ireland, New Zealand and Pakistan. Furthermore, the study found a negative correlation between the perception of school leaders and inspectors regarding the quality of inspection practices and their perception of the impact of inspection. Thus, it is imperative to establish improved avenues of communication to facilitate heightened awareness among school leaders regarding the efficacy of inspectors’ work and the comprehensive measures implemented by inspectorates to ensure the quality of their own practices. This initiative will foster enhanced trust between school leaders and inspectors, consequently amplifying the overall influence of school inspections.
Supporting service integration through early childhood education: challenges and opportunities in regional contexts
Authors: Neilsen-Hewett Cathrine , de Rosnay Marc , Singleton Janine , Stouse-Lee Kim
Published: September 2023

Introduction

Children and families residing in regional Australia experience higher rates of vulnerabilities coupled with inadequate access to the early childhood health and early intervention services which pose increased risk to their health, development and wellbeing. The current study was designed to respond to the inherent complexity of supporting effective integrated service provision in regional communities, with a view to develop a model of effective service integration that leverages the capacity and opportunity of universal early childhood education (ECE) provision.

Method

The study adopted a qualitative multiple case study design to explore the perceptions of ECE professionals across six regional ECE services and two early intervention professionals operating from a regional early childhood intervention (ECI) organization. Data included an initial audit of the service system landscape coupled with facilitated discussions (focus groups and interviews) to identify facilitators and challenges to service integration and current patterns of service usage and engagement.

Results

Findings highlighted the foundational importance of relationships for establishing trust, engagement and service sustainability, as well as the need for embedding structural supports, including the professionalization of educators, the utilization of a key worker model, and staff retention. Systemic constraints, including limitations and inconsistencies in community infrastructure, program atrophy, and the complexity of referral systems, were seen to undermine effective service integration.

Discussion

Findings speak to the potentiality of the ECE context as a hub for effective service integration within a functional practice framework for ECE. We conclude by offering a suggested model to ensure service connections, and enhance professional capacity and sustainability.
Assessing the role of central lymph node ratio in predicting recurrence in N1a low-to-intermediate risk papillary thyroid carcinoma
Authors: Ma Teng , Cui Jian , Shi Peng , Liang Mei , Song Wenxiao , Zhang Xueyan , Wang Lulu , Shi Yafei
Published: September 2023

Introduction

Lymph node metastasis in patients with papillary thyroid carcinoma (PTC) is associated with postoperative recurrence. Recently, most studies have focused on the evaluation of recurrence in patients with late-stage PTC, with limited data on those with early-stage PTC. We aimed to assess the relationship between lymph node ratio (LNR) and recurrence in low-to-intermediate-risk patients and validate its diagnostic efficiency in both structural (STR) and biochemical recurrence (BIR).

Methods

Clinical data of patients with PTC diagnosed at the Affiliated Hospital of Jining Medical University were retrospectively collected. The optimal LNR cut-off values for disease-free survival (DFS) were determined using X-tile software. Predictors were validated using univariate and multivariate Cox regression analyses.

Results

LNR had a higher diagnostic effectiveness than metastatic lymph nodes in patients with low-to-intermediate recurrence risk N1a PTC. The optimal LNR cutoff values for STR and BIR were 0.75 and 0.80, respectively. Multivariate Cox regression analysis showed that LNR≥0.75 and LNR≥0.80 were independent factors for STR and BIR, respectively. The 5-year DFS was 90.5% in the high LNR (≥0.75) and 96.8% in low LNR (<0.75) groups for STR. Regarding BIR, the 5-year DFS was 75.7% in the high LNR (≥0.80) and 86.9% in low LNR (<0.80) groups. The high and low LNR survival curves exhibited significant differences on the log-rank test.

Conclusion

LNR was associated with recurrence in patients with low-to-intermediate recurrence risk N1a PTC. We recommend those with LNR≥0.75 require a comprehensive evaluation of lateral neck lymphadenopathy and consideration for lateral neck dissection and RAI treatment.
Better oral hygiene is associated with a reduced risk of osteoporotic fracture: a nationwide cohort study
Authors: Park Jung-Hyun , Park Moo-Seok , Kim Hyung-Jun , Lee Heajung , Kim Jin-Woo , Song Tae-Jin
Published: September 2023

Background

The aim of this study was to examine the longitudinal association between oral health parameters and osteoporotic fracture.

Methods

The study included participants who received oral health screening by dentists from the National Health Screening cohort database of Korea between 2003 and 2006. The primary outcome was osteoporotic fracture occurrence, which was defined using specific international classification of diseases-10 codes; vertebral fracture (S22.0, S22.1, S32.0, S32.7, T08, M48.4, M48.5, and M49.5), hip fracture (S72.0 and S72.1), distal radius fracture (S52.5 and S52.6), and humerus fracture (S42.2 and S42.3). The presence of periodontitis and various oral health examination findings, such as missing teeth, caries, frequency of tooth brushing, and dental scaling, were analyzed using a Cox proportional hazard model to assess their association with osteoporotic fracture occurrence.

Results

The analysis included a total of 194,192 participants, among whom 16,683 (8.59%) developed osteoporotic fracture during a median follow-up of 10.3 years. Poor oral health status, including periodontitis (adjusted hazard ratio [aHR]: 1.09, 95% confidence interval [CI]: 1.01–1.18, p = 0.039), a higher number of missing teeth (≥15; aHR: 1.59, 95% CI: 1.45–1.75, p < 0.001), and dental caries (≥6; aHR: 1.17, 95% CI: 1.02–1.35, p = 0.030), was associated with an increased risk of osteoporotic fracture. On the other hand, better oral hygiene behaviors such as brushing teeth frequently (≥3 times per day; aHR: 0.82, 95% CI: 0.78–0.86, p < 0.001) and having dental scaling within 1 year (aHR: 0.87, 95% CI: 0.84–0.90, p < 0.001) were negatively associated with the occurrence of osteoporotic fracture.

Conclusion

The study found that poor oral health, such as periodontitis, missing teeth, and dental caries, was associated with an increased risk of osteoporotic fracture. Conversely, good oral hygiene behaviors like frequent teeth brushing and dental scaling within 1 year were associated with a reduced risk. Further research is needed to confirm this association.
In-situ stress prediction in ultra-deep carbonate reservoirs of Fuman Oilfield, Tarim Basin of China
Authors: Yin Guoqing , Wu Kongyou , Ju Wei , Qin Yun , Qian Ziwei , Xu Ke , Lu Zhongyuan , Wang Peng , Liang Xiaobai , Liang Yan
Published: September 2023

The Fuman Oilfield in Tarim Basin has great potential for ultra-deep carbonate oil and gas resources, and is an important area for future storage and production increase. The present-day in-situ stress field is critical during the exploration and development. However, no systemic investigations have been carried out in this oilfield. Therefore, in this study, the present-day in-situ stress field in the Ordovician carbonate reservoir is predicted and analyzed based on well log calculation and geomechanical numerical modeling. The results indicate that, 1) NE-SW-trending is the dominant horizontal maximum principal stress (SHmax) orientation. The vertical principal stress is the maximum principal stress, showing the Ordovician reservoir is under a normal faulting stress regime. 2) The distribution of in-situ stresses in the Ordovician carbonate reservoir is heterogeneous, which is mainly controlled burial depth and fault/fracture development. High stress magnitudes in the Yingshan Formation are mainly in the southeastern part of Fudong area, Fuman Oilfield. The present-day horizontal differential stress mainly ranges from 27 MPa to 30 MPa in the Yingshan carbonate reservoir. iii) Natural fractures are generally stale under the present-day in-situ stress state. Fractures that parallel to the SHmax orientation with high fracture dip angle are easier to be reactivated. The results are expected to provide geomechanical references for further oil and gas development in the Fuman Oilfield of Tarim Basin.
Virtual inertia analysis of photovoltaic energy storage systems based on reduced-order model
Authors: Li Guanyang , Wang Junfei , Wang Xuanlin , Zhang Li
Published: September 2023

The problem of non-ideal inertia of the photovoltaic energy storage system (PVESS) may occur due to unreasonable voltage control parameters. In response to this issue, this paper establishes an equivalent reduced-order model (EROM) for PVESS. This EROM considers the current control loop, voltage control loop and the virtual inertia control loop based on low-pass filter. This low-pass filter can effectively enhance the system’s virtual inertia. Since the output impedance of this EROM can visually reflect the external characteristics of the virtual inertia control loop, it is suitable for inertia analysis of PVESS. Furthermore, the impact of voltage control parameters and low-pass filter bandwidth on the system’s inertia is discussed from the perspective of the frequency response of the output impedance. Finally, the switch model of the PVESS is built on the RT-BOX hardware-in-the-loop experimental platform. The validity of the EROM and theoretical analysis is verified by several sets of experimental results.
Biogeographic patterns and environmental drivers of species richness in the globally distributed Millettioid/Phaseoloid clade (Fabaceae, subfamily Papilionoideae)
Authors: Oyebanji Oyetola O. , Onditi Kenneth O. , Azevedo Josué A. R. , Rahaingoson Fabien R. , Nneji Lotanna M. , Adeleye Matthew. A. , Stull Gregory W. , Zhang Rong , Yi Ting-Shuang
Published: September 2023

Introduction

The Millettioid/Phaseoloid (MP) clade of Fabaceae is globally distributed, economically important, and highly diverse, making it an attractive system for studying biogeographic and macroecological patterns at a global scale. We conducted the first global macroecological study to map and explore the environmental drivers of the MP clade's species richness patterns.

Methods

We compiled 116,212 species occurrences (161 genera) for the MP clade and 20 environmental variables (19 bioclimatic variables and elevation). Geospatial analyses were performed to estimate species richness patterns and biogeographic heterogeneity. The effects of environmental variables on the species richness of the MP clade were measured through multiple regression models.

Results

Our study identified the megathermal regions as hotspots of species richness for the MP clade. While species distributions and richness largely fit the latitudinal diversity gradient pattern, there was a significant negative relationship between the species richness of the MP clade along the latitude and longitude. The Afrotropic biogeographic realm had the highest alpha diversity (~36%); in terms of biome types, tropical and subtropical moist broadleaf forests had the highest alpha diversity (25%), while the beta diversity revealed a high dispersal rate and habitat tracking. Furthermore, the species richness was positively influenced by multiple climatic factors, with the mean diurnal range of temperatures and precipitation in the warmest quarter having strongest influence.

Discussion

Overall, the staggering species richness patterns could be explained by multiple diversity gradient hypotheses. Particularly, colder climates play a crucial role in shaping the species richness pattern by limiting the ecological opportunities for MP clade species in the higher latitudes of the Northern Hemisphere. This suggests that the species richness patterns of the MP clade can be described as "when dispersal meets adaptation." Our study provides a new basis for identifying priority regions for conservation of legumes.
Life on the wall: the diversity and activity of microbes on 13th – century AD. Lan Na mural painting
Authors: Sansupa Chakriya , Suphaphimol Nattaphon , Nonthijun Paradha , Ronsuek Teewararat , Yimklan Saranphong , Semakul Natthawat , Khrueraya Thapakorn , Suwannarach Nakarin , Purahong Witoon , Disayathanoowat Terd
Published: September 2023

Diverse microorganisms from the three domains of life (Archaea, Bacteria, and Eukaryota) cause deterioration in mural paintings worldwide; however, few studies have simultaneously targeted these three domains. This study aims to survey the microbiome and its potential for biodeterioration on unpreserved Lan Na mural paintings in Sean Khan temple, Chiang Mai, Thailand. The overview of the archaeal, bacterial, and fungal communities was reported by Illumina sequencing, whereas the potential for deterioration was revealed by culturable techniques and a literature search. The abundant microbes reported in this study were also found in other ancient mural paintings worldwide. Halococcus, a salt-tolerant archaeon, as well as the eubacterial genus Crossiella dominated the prokaryotic community. On the other hand, the main fungal group was the genus Candida (Ascomycota). However, a low number of fungi and bacteria were isolated. Most of the isolates showed the ability to survive in the drought conditions of mural paintings but could not perform discoloration activities. The deterioration activity mainly affected calcium compounds, which are the main components of painting substrates. Aspergillus and several bacterial isolates could dissolve calcium compounds, but only Trichaptum species could induce crystal formation. These results suggest that deterioration of painting substrate should be taken into consideration in addition to deterioration of color in mural paintings. For the Lan Na painting in Sean Khan temple, the plaster is the prime target for biodeterioration, and thus we suggest that the preservation effort should focus on this component of the mural painting.
Osimertinib inhibits brain metastases and improves long-term survival in a patient with advanced squamous cell lung cancer: a case report and literatures review
Authors: Zhang Zhiqin , Lin Jiamao , Yang Linke , Li Yang
Published: September 2023

Background

Squamous cell carcinoma (SCC) is one of the most common subtypes of non-small cell lung cancer, but its treatment options remain limited. Epidermal growth factor receptor (EGFR)–tyrosine kinase inhibitors (TKIs) have limited efficacy in the treatment of lung SCC. Here, we report an SCC patient who developed EGFR-T790M mutation and showed gefitinib resistance achieved an extremely long survival by taking Osimertinib alternatively.

Case summary

A patient, 66-year-old non-smoking and drinking male with advanced SCC who was deemed inoperable at the time of diagnosis. The first genetic testing showed deletion mutation of exon 19 of EGFR. The patient was then treated with gefitinib with no significant efficacy. EGFR-T790M mutation was found in the second genetic test. The treatment regimen was changed to radiotherapy with Osimertinib, and the patient’s primary lesion and the brain metastases were well controlled.

Conclusion

This typical case highlights the important role of Osimertinib in patients with SCC carrying EGFR mutations.
Oral squamous cell carcinoma – do we always need elective neck dissection? evaluation of clinicopathological factors of greatest prognostic significance: a cross-sectional observational study
Authors: Michcik Adam , Polcyn Adam , Sikora Maciej , Wach Tomasz , Garbacewicz Łukasz , Drogoszewska Barbara
Published: September 2023

The aim of the study was to find clinical and pathological factors with the greatest prognostic significance in patients with OSCC. The analysis included 125 patients grouped according to the tumor primary site (TPS): the floor of the mouth (FOM), tongue (TC) and retromolar triangle (RMT). Grading (G), tumor size (pT), nodal metastases (NM), local recurrence (LR), nodal recurrence (NR), perineural invasion (PNI), lymphovascular invasion (LVI), extranodal extension (pENE), and nodal yield (NY) were evaluated in each group.

Results

With regard to TPS, FOM appeared to be the most metastatic. However, the recurrence rate was similar to TC tumors, which were characterized by higher G than those in other locations. When analyzing G, the highest percentage of LR (40.5%) and NM (34.5%) was observed among patients with G2. As G increased, so did the number of pENE G1 – 7.4%; G2 – 31%; G3 – 35.7%; LVI: G1 – 25.9%; G2 – 50%; G3 – 57.1%; PNI: G1 – 29.6%; G2 – 47.6%; G3 – 92.9%; NR G1 – 14.8%; G2 – 32.1%; G3 – 21.4%. Grading did not affect the type of growth and did not directly affect the occurrence of NR. pT and DOI increased the frequency of NM but we did not observe any effect of pT and DOI on LR, PNI, and LVI. NY in the study group did not increase the risk of NR.

Conclusion

Tumor primary sites within the FOM, TC, and pT classification are the factors that increase the risk of NM and LR. However, apart from the primary site predisposing to the occurrence of NM, the histological structure of the tumor turned out to be the most important feature affecting the patient’s prognosis. The number of cases of pENE+, LVI+, PNI+, NM+, and NR+ increased with the increase in G. Although the pT, DOI increased the frequency of NM, we did not observe the effect of the pT and DOI on LR, PNI and LVI. Thus, even in the case of a small tumor of the FOM and TC with at least G2, elective neck dissection should be performed each time.
Caregiver’s quality of life in advanced cancer: validation of the construct in a real-life setting of early palliative care
Authors: Borelli Eleonora , Bigi Sarah , Potenza Leonardo , Gilioli Fabio , Efficace Fabio , Porro Carlo Adolfo , Luppi Mario , Bandieri Elena
Published: September 2023

Introduction

Early palliative care (EPC) improves the quality of life (QoL) of advanced cancer patients and their caregivers. The increasingly widespread use of this care model requires the development of measures supporting its interventions. Although the construct of patient’s QoL has been extensively investigated and several QoL measures have been further validated, there is a paucity of data concerning the QoL of the caregiver. In 2018, McDonald and colleagues addressed this issue by interviewing 23 primary caregivers of advanced cancer patients who participated in an EPC randomized clinical trial to understand their perspective on the QoL construct. The Authors identified six major dimensions associated with the construct of caregiver’s QoL. The present retrospective study aimed to validate these dimensions on a larger sample and in a real-life EPC setting.

Methods

Previously collected reports from 137 primary caregivers of advanced cancer patients on EPC answering questions about their experience with this care model were qualitatively analyzed through a deductive, thematic approach to identify and confirm the six dimensions constituting the construct of interest based on McDonald’s and colleagues’ results.

Results

The six dimensions (“living in the patient’s world”, “burden of illness and caregiving”, “assuming the caregiver role”, “renegotiating relationships”, “confronting mortality”, and “maintaining resilience”) were consistently found in the reports from primary caregivers in a real-life EPC setting, confirming to be significant themes associated to their QoL.

Conclusion

A definite and recurrent construct of primary caregiver’s QoL as described by McDonald and colleagues was also found in a larger sample and in a real-life EPC setting. Thus it may lay the groundwork for the development of a dedicated questionnaire.
The role of tumor-associated macrophages in glioma cohort: through both traditional RNA sequencing and single cell RNA sequencing
Authors: Hou Yunan , Qiu Wenjin , Ling Yuanguo , Qi Xiaolan , Liu Jian , Yang Hua , Chu Liangzhao
Published: September 2023

Gliomas are the leading cause in more than 50% of malignant brain tumor cases. Prognoses, recurrences, and mortality are usually poor for gliomas that have malignant features. In gliomas, there are four grades, with grade IV gliomas known as glioblastomas (GBM). Currently, the primary methods employed for glioma treatment include surgical removal, followed by chemotherapy after the operation, and targeted therapy. However, the outcomes of these treatments are unsatisfactory. Gliomas have a high number of tumor-associated macrophages (TAM), which consist of brain microglia and macrophages, making them the predominant cell group in the tumor microenvironment (TME). The glioma cohort was analyzed using single-cell RNA sequencing to quantify the genes related to TAMs in this study. Furthermore, the ssGSEA analysis was utilized to assess the TAM-associated score in the glioma group. In the glioma cohort, we have successfully developed a prognostic model consisting of 12 genes, which is derived from the TAM-associated genes. The glioma cohort demonstrated the predictive significance of the TAM-based risk model through survival analysis and time-dependent ROC curve. Furthermore, the correlation analysis revealed the significance of the TAM-based risk model in the application of immunotherapy for individuals diagnosed with GBM. Ultimately, the additional examination unveiled the prognostic significance of PTX3 in the glioma group, establishing it as the utmost valuable prognostic indicator in patients with GBM. The PCR assay revealed the PTX3 is significantly up-regulated in GBM cohort. Additionally, the assessment of cell growth further confirms the involvement of PTX3 in the GBM group. The analysis of cell proliferation showed that the increased expression of PTX3 enhanced the ability of glioma cells to proliferate. The prognosis of glioblastomas and glioma is influenced by the proliferation of tumor-associated macrophages.
Surgical options for patients with early-stage breast cancer and pathogenic germline variants: an oncologist perspectives
Authors: Abdel-Razeq Hikmat
Published: September 2023

Breast cancer continues to be the most common cancer diagnosed among women worldwide. Family history of breast cancer is frequently encountered, and 5-15% of patients may carry inherited pathogenic germline variants, identification of which can be helpful for both; patients themselves and their unaffected close relatives. The availability and affordability of molecular diagnostics, like next generation sequencing (NGS), had resulted in wider adoption of such technologies to detect pathogenic variants of cancer-predisposing genes. International guidelines had recently broadened the indications for germline genetic testing to include much more patients, and also expanded the testing to include multi-gene panels, while some professional societies are calling for universal testing of all newly diagnosed patients with breast cancer, regardless of their age, personal or family history. The risk of experiencing a contralateral breast cancer (CBC) or ipsilateral recurrence, is well known. Such risk is highest with variants like BRCA1 and BRCA2, but less well-studied with other less common variants. The optimal local therapy for women with BRCA-associated breast cancer remains controversial, but tends to be aggressive and may involve bilateral mastectomies, which may not have any survival advantage. Additionally, surgical management of unaffected women, known to carry a pathogenic cancer-predisposing gene, may vary from surveillance to bilateral mastectomies, too. The oncological safety, and the higher satisfaction of unaffected women and patients with new surgical techniques, like the skin-sparing (SSM) and nipple-sparing (NSM) mastectomies, eased up the process of counselling. In this review, we address the oncological safety of less aggressive surgical options for both; patients and unaffected carriers.
LTe2 induces cell apoptosis in multiple myeloma by suppressing AKT phosphorylation at Thr308 and Ser473
Authors: Zhang Yuanjiao , Qian Jiacheng , Jiang Mingmei , Yang Shu , Zhou Lianxin , Zhang Qin , Lin Liping , Yang Ye
Published: September 2023

Multiple myeloma (MM) is a highly heterogeneous hematological malignancy originating from B lymphocytes, with a high recurrence rate primarily due to drug resistance. 2-((1H-indol-3-yl)methyl)-3-((3-((1H-indol-3-yl)methyl)-1H-indol-2-yl)methyl)-1H-indole (LTe2), a tetrameric indole oligomer, possesses a wide range of anticancer activities through various mechanisms. Here, we aim to explore the anti-tumor efficiency and potential downstream targets of LTe2 in MM. Its bioactivity was assessed by employing MTT assays, flow cytometry, and the 5TMM3VT mouse model. Additionally, transcriptomic RNA-seq analysis and molecular dynamics (MD) experiments were conducted to elucidate the mechanism underlying LTe2 induced MM cell apoptosis. The results demonstrated that LTe2 significantly inhibited MM cell proliferation both in vitro and in vivo, and revealed that LTe2 exerts its effect by inhibiting the phosphorylation of AKT at the Thr308 and Ser473 sites. In summary, our findings highlight the potential of LTe2 as a novel candidate drug for MM treatment and provided a solid foundation for future clinical trials involving LTe2.
Recent advances in the study of anesthesia-and analgesia-related mechanisms of S-ketamine
Authors: Zhou Jian-shun , Peng Guan-fa , Liang Wei-dong , Chen Zhen , Liu Ying-ying , Wang Bing-yu , Guo Ming-ling , Deng Yun-ling , Ye Jun-ming , Zhong Mao-lin , Wang Li-feng
Published: September 2023

Ketamine is a racemic mixture of equal amounts of R-ketamine and S-ketamine and is well known to anesthesiologists for its unique dissociative anesthetic properties. The pharmacological properties of ketamine, namely, its sympathetic excitation, mild respiratory depression, and potent analgesia, are still highly valued in its use as an anesthetic for some patients. In particular, since its advent, S-ketamine has been widely used as an anesthetic in many countries due to its increased affinity for NMDA receptors and its enhanced anesthetic and analgesic effects. However, the anesthetic and analgesic mechanisms of S-ketamine are not fully understood. In addition to antagonizing NMDA receptors, a variety of other receptors or channels may be involved, but there are no relevant mechanistic summaries in the literature. Therefore, the purpose of this paper is to review the mechanisms of action of S-ketamine on relevant receptors and systems in the body that result in its pharmacological properties, such as anesthesia and analgesia, with the aim of providing a reference for its clinical applications and research.
Identification of prognostic and diagnostic signatures for cancer and acute myocardial infarction: multi-omics approaches for deciphering heterogeneity to enhance patient management
Authors: Yuan Na , Pan Hai-Hua , Liang Yan-Shan , Hu Hui-Lin , Zhai Chang-Lin , Wang Bo
Published: September 2023

Patients diagnosed with cancer face an increased risk of cardiovascular events in the short term, while those experiencing acute myocardial infarction (AMI) have a higher incidence of cancer. Given limitations in clinical resources, identifying shared biomarkers offers a cost-effective approach to risk assessment by minimizing the need for multiple tests and screenings. Hence, it is crucial to identify common biomarkers for both cancer survival and AMI prediction. Our study suggests that monocyte-derived biomarkers, specifically WEE1, PYHIN1, SEC61A2, and HAL, hold potential as predictors for cancer prognosis and AMI. We employed a novel formula to analyze mRNA levels in clinical samples from patients with AMI and cancer, resulting in the development of a new risk score based on expression profiles. By categorizing patients into high-risk and low-risk groups based on the median risk score, we observed significantly poorer overall survival among high-risk patients in cancer cohorts using Kaplan-Meier analysis. Furthermore, calibration curves, decision curve analysis (DCA), and clinical impact curve analyses provided additional evidence supporting the robust diagnostic capacity of the risk score for AMI. Noteworthy is the shared activation of the Notch Signaling pathway, which may shed light on common high-risk factors underlying both AMI and cancer. Additionally, we validated the differential expression of these genes in cell lines and clinical samples, respectively, reinforcing their potential as meaningful biomarkers. In conclusion, our study demonstrates the promise of mRNA levels as biomarkers and emphasizes the significance of further research for validation and refinement.
Head-Toes-Knees-Shoulders task and EF in two samples of adolescents in Brazil and United States
Authors: Fernandes Valter R. , Becker Derek R. , McClelland Megan M. , Deslandes Andrea C.
Published: September 2023

Executive function (EF) is a foundational cognitive construct, which is linked to better cognitive and physical health throughout development. The present study examines the construct validity of an EF task, the Head-Toes-Knees-Shoulders task (HTKS) that was initially developed for young children, in a sample of adolescents. We investigate the initial validity and range in scores between 54 adolescents from Brazil (mean age 12.58) and 56 US adolescents (mean age 12.48) from different socioeconomic contexts. Results indicated that the HTKS showed sufficient variability in both samples, especially for a measure of HTKS efficiency (completion time divided by the total score). The US sample performed better on all cognitive measures. For the Brazilian sample, regression models controlling for age and sex showed a significant relationship between the digit span working memory task, the HTKS total score, and the HTKS efficiency score. The Heart and Flowers cognitive flexibility measure was also included as an independent variable only for the Brazil sample, showing a significant relationship with both HTKS scores. For the US sample, results showed that only the HTKS efficiency score was significantly related to the digit span working memory task. This study highlights the importance of cognitive efficiency measures to achieve greater validity, as they can assess a broader range of performance with different populations. The HTKS showed good ecological validity with two adolescent samples, as it differentiated between populations with high and low socioeconomic status from different cultural contexts.
Abuse of power in the disciplinary actions of a state psychology licensing board: inequitable outcomes and early career psychologists
Authors: Faber Sonya C. , Wu Edward , Bartlett Amy
Published: September 2023

The field of psychology has established high professional standards which have become a cornerstone of the practice of psychology. However, powerful boards tasked with administering these standards can operate with little oversight, making it difficult to monitor whether these institutions are operating in a fair and impartial way. In particular, early-career psychologists who have less experience and power in their initial years of independent practice may be singularly vulnerable as they have relatively little experience to navigate the profession, including fielding complaints that may be made against them to a licensing board. While it is essential to ensure early-career psychologists are upholding their commitments to the practice, there are risks in policing their activities without orienting toward growth, learning, and professional development. Even the smallest disciplinary action may never be expunged from a psychologist’s record, resulting in long-term implications for insurance coverage, reputation and future professional viability in the field. Overly-punitive approaches can be distressing or even traumatizing. In this paper, we examine disciplinary actions of the Kentucky Board of Examiners of Psychology (KBEP) from the years 2000 to 2020 (N = 65) to determine the methodology by which the Board administers its oversight function. We analyze the nature of the discipline received (fines, suspensions, continuing education, supervision) revealing a two-tiered system of punishments, and provide context regarding the nature of the disciplinary process and its impacts. We report on qualitative interviews of early career psychologists subject to disciplinary actions by the Board, and psychologists who supervised early career psychologists investigated by the Board. We compare legislation governing KBEP and make comparisons to the workings of licensing boards in three other states. Using these findings, we make recommendations for revisions to the applicable legislation and administrative processes of the Board to establish an improved balance between public safety, the well-being of new psychologists, equity considerations such as race, and the development of the practice of psychology in Kentucky. This work brings to light previously unexamined injustices that can knowingly or unknowingly be perpetuated by licensing Boards, and can be used to inform the creation of more just, balanced and inclusive professional Boards.