bluuebunny/crossref_metadata_2025 huggingface dataset:
Main Determinants Affecting the Antiproliferative Activity of Stilbenes and Their Gut Microbiota Metabolites in Colon Cancer Cells: A Structure–Activity Relationship Study
Authors: González-Sarrías Antonio , Espín-Aguilar Juan Carlos , Romero-Reyes Salvador , Puigcerver Julio , Alajarín Mateo , Berná José , Selma María Victoria , Espín Juan Carlos
Published: December 2022

trans-Resveratrol can be catabolized by the gut microbiota to dihydroresveratrol, 3,4′-dihydroxy-trans-stilbene, lunularin, and 4-hydroxydibenzyl. These metabolites can reach relevant concentrations in the colon. However, not all individuals metabolize RSV equally, as it depends on their RSV gut microbiota metabotype (i.e., lunularin producers vs. non-producers). However, how this microbial metabolism affects the cancer chemopreventive activity of stilbenes and their microbial metabolites is poorly known. We investigated the structure–antiproliferative activity relationship of dietary stilbenes, their gut microbial metabolites, and various analogs in human cancer (Caco-2 and HT-29) and non-tumorigenic (CCD18-Co) colon cells. The antiproliferative IC50 values of pterostilbene, oxy-resveratrol, piceatannol, resveratrol, dihydroresveratrol, lunularin, 3,4′-dihydroxy-trans-stilbene, pinosylvin, dihydropinosylvin, 4-hydroxy-trans-stilbene, 4-hydroxydibenzyl, 3-hydroxydibenzyl, and 4-trans-stilbenemethanol were calculated. IC50 values were correlated with 34 molecular characteristics by bi- and multivariate analysis. Little or no activity on CCD18-Co was observed, while Caco-2 was more sensitive than HT-29, which was explained by their different capacities to metabolize the compounds. Caco-2 IC50 values ranged from 11.4 ± 10.1 μM (4-hydroxy-trans-stilbene) to 73.9 ± 13.8 μM (dihydropinosylvin). In HT-29, the values ranged from 24.4 ± 11.3 μM (4-hydroxy-trans-stilbene) to 96.7 ± 6.7 μM (4-hydroxydibenzyl). At their IC50, most compounds induced apoptosis and arrested the cell cycle at the S phase, pterostilbene at G2/M, while 4-hydroxy-trans-stilbene and 3,4′-dihydroxy-trans-stilbene arrested at both phases. Higher Connolly values (larger size) hindered the antiproliferative activity, while a lower pKa1 enhanced the activity in Caco-2, and higher LogP values (more hydrophobicity) increased the activity in HT-29. Reducing the styrene double bond in stilbenes was the most critical feature in decreasing the antiproliferative activity. These results (i) suggest that gut microbiota metabolism determines the antiproliferative effects of dietary stilbenes. Therefore, RSV consumption might exert different effects in individuals depending on their gut microbiota metabotypes associated with RSV metabolism, and (ii) could help design customized drugs with a stilbenoid and (or) dibenzyl core against colorectal cancer.
Germ Cell Isolation and Cryopreservation from Reproductive Organs of Brown Mealworm
Authors: Byeun Do Gyeung , Moon Byoung-San , Lee Seungki , Choi Jung Kyu
Published: December 2022

This study aimed to isolate and freeze germ cells from the superior brown mealworm. Styrofoam diet changes were observed for 20 days to determine whether mealworms were useful insects for decomposing Styrofoam. The average weight of mealworms before the Styrofoam diet was 500 mg, which decreased to 336 mg at D20 after their diet. To preserve mealworms with excellent Styrofoam-degrading ability, we first isolated the reproductive organs of mealworms, testes, ovaries, sperms, and ovarioles. Morphologically, male and female adult brown mealworms were distinguished according to the presence or absence of a protrusion at the tip of the fifth segment of the abdomen. Sperms and ovarioles were observed in anatomically isolated testes and ovaries. We compared mechanical and enzymatic (collagenase I) methods to effectively isolate ovarioles from adult female brown mealworms. For the enzymatic method, most were torn and burst as the membrane of the ovarioles was damaged by collagenase I, unlike the mechanical method. To preserve the superior genetic resources of mealworms, we cryopreserved the ovaries of female brown mealworms using slow-freezing and vitrification. Histological analysis showed that the yolk sac was completely damaged in the ovaries after slow-freezing. However, only partial damage was achieved in the vitrification group compared to the control group (no freezing). The newly developed vitrification method with alginate-encapsulated ovarioles maintained the yolk sac in the ovarioles but was evenly distributed. These results provide basic data for reproductive studies of other useful insects and contribute to the biobanking and fertility preservation of superior mealworm germ cells and endangered insects.
The Association between the Distribution of Resin Beads and the Emergence of Sirex noctilio on Red Pine in North America
Authors: Faal Hajar , Teale Stephen A.
Published: December 2022

This study examined the relationships of the abundance and distribution of resin beads (signs of Sirex noctilio parent female ovipositor activity) with the abundance and distribution of emerging progeny of S. noctilio, S. nigricornis and their parasitoid Ibalia leucospoides. S. noctilio is native to Europe and is an invasive pest of pines in the Southern Hemisphere and North America; S. nigricornis is native to North America and is a secondary pest of dying pines. I. leucospoides is a parasitoid that has been widely deployed for biological control of S. noctilio. This study aimed to determine if the distribution of resin beads is associated with the height, diameter, or cardinal direction on red pines, Pinus resinosa, as well as the distribution of wood wasp and parasitoid emergence. Our results showed that among log sections taken at five heights, resin beads were most abundant on the north, east, and south sides of logs and mid log at 4.5 m above the ground. Emergence of S. noctilio was most abundant only from logs with more than five resin beads per square meter, while diameter and height were not contributing factor. None of variables evaluated (resin bead densities, height, and diameter) had significant effects on the emergence of S. nigricornis and I. leucospoides. These findings help clarify the biological significance of resin beads as indicators of S. noctilio colonization of host trees in North America.
Karyotypes and COI Gene Sequences of Chironomus sp. Le1 (Kiknadze and Salova, 1996), Ch. laetus (Belyanina and Filinkova, 1996) and Their Hybrid from the Yamal Peninsula, Arctic Zone of Russia
Authors: Bolshakov Viktor , Prokin Alexander , Pavlov Dmitry , Akkizov Azamat , Movergoz Ekaterina
Published: December 2022

The study of the biological diversity of the Arctic zone yields intriguing results. Initial research on the lakes of the Yamal Peninsula resulted in the identification of Chironomus laetus and the hybrid Ch. laetus × Ch. sp. Le1. To avoid misidentification, we used morphological, cytogenetic, and molecular genetic approaches. By cytogenetics, in Ch. sp. Le1, seven banding sequences were found: Le1A1, Le1B1, Le1C1, Le1D1, Le1E1, Le1F1, and Le1G1. The karyotype of Ch. laetus was mapped for the first time; it is the first species with the arm combinations AE BC DF G. We propose the name of a new cytocomplex—“laetus”. DNA-barcoding of the COI gene was carried out for Ch. laetus and Ch. laetus × Ch. sp. Le1 for the first time. The estimated genetic distance between the sequences of Ch. laetus and Ch. riihimakiensis is 2.3–2.5%. The high similarity in morphology, banding sequences, and the possibility of hybridization indicate a close relationship between Ch. laetus and Ch. sp. Le1, which is assumed to be the northern variant of Ch. riihimakiensis. Molecular genetic data suggests the presence of a subgroup with Ch. laetus.
Impact of Preoperative Handgrip Strength on Postoperative Outcome after Radical Gastrectomy for Gastric Cancer Patients
Authors: Matsui Ryota , Inaki Noriyuki , Tsuji Toshikatsu , Momosaki Ryo , Fukunaga Tetsu
Published: December 2022

In this study, we investigated whether preoperative low-handgrip strength (HGS) defined by the Asian working group for sarcopenia could be a predictor of postoperative outcomes in patients with gastric cancer. A total of 327 patients who underwent radical gastrectomy for c-stage I–III primary gastric cancer with pre-operative HGS records were included. The cut-off values of HGS were defined as 28 kg for males and 18 kg for females, with values below and above the cut-off defined as low-HGS and high-HGS, respectively. The primary outcome was infectious complications. We compared the postoperative outcomes of the groups after adjusting for the background using propensity score matching. Of the 327 patients, 246 (75.2%) and 81 (24.8%) were in the high and low-HGS groups, respectively. After adjusting for background, there were 57 patients in both groups. After matching, the low-HGS group had significantly more infectious complications (17.5% vs. 1.8%, p = 0.008). Multivariate analysis of infectious complications in the low-HGS group demonstrated chronic kidney disease and diabetes as independent risk factors (odds ratio 4.390, 95% confidence interval 1.120–17.20, p = 0.034). Preoperative low-HGS according to the Asian criteria was associated with infectious complications after gastrectomy. Chronic kidney disease and diabetes were independent risk factors for infectious complications among patients with low-HGS.
Prognosis of Spontaneous Pneumothorax/Pneumomediastinum in Coronavirus Disease 2019: The CoBiF Score
Authors: Woo Wongi , Kipkorir Vincent , Marza Adina Maria , Hamouri Shadi , Albawaih Omar , Dhali Arkadeep , Kim Wooshik , Udwadia Zarir F. , Nashwan Abdulqadir J. , Shaikh Nissar , Belletti Alessandro , Landoni Giovanni , Palumbo Diego , Swed Sarya , Sawaf Bisher , Buonsenso Danilo , Pimenta Inês , Gonzalez Filipe André , Fiorentino Giuseppe , Rashid Ali Muhammad Redzwan S. , Quincho-Lopez Alvaro , Javanbakht Mohammad , Alhakeem Ayat , Khan Muhammad Mohsin , Shah Sangam , Rafiee Moezedin Javad , Padala Sri Rama Ananta Nagabhushanam , Diebel Sebastian , Song Seung Hwan , Kang Du-young , Moon Duk Hwan , Lee Hye Sun , Yang Juyeon , Flower Luke , Yon Dong Keon , Lee Seung Won , Shin Jae Il , Lee Sungsoo ,   International COVID-19 Pneumothorax Working Group (ICP-WG)
Published: December 2022

Objectives: Pneumothorax and pneumomediastinum are associated with high mortality in invasively ventilated coronavirus disease 2019 (COVID-19) patients; however, the mortality rates among non-intubated patients remain unknown. We aimed to analyze the clinical features of COVID-19-associated pneumothorax/pneumomediastinum in non-intubated patients and identify risk factors for mortality. Methods: We searched PubMed Scopus and Embase from January 2020 to December 2021. We performed a pooled analysis of 151 patients with no invasive mechanical ventilation history from 17 case series and 87 case reports. Subsequently, we developed a novel scoring system to predict in-hospital mortality; the system was further validated in multinational cohorts from ten countries (n = 133). Results: Clinical scenarios included pneumothorax/pneumomediastinum at presentation (n = 68), pneumothorax/pneumomediastinum onset during hospitalization (n = 65), and pneumothorax/pneumomediastinum development after recent COVID-19 treatment (n = 18). Significant differences were not observed in clinical outcomes between patients with pneumomediastinum and pneumothorax (±pneumomediastinum). The overall mortality rate of pneumothorax/pneumomediastinum was 23.2%. Risk factor analysis revealed that comorbidities bilateral pneumothorax and fever at pneumothorax/pneumomediastinum presentation were predictors for mortality. In the new scoring system, i.e., the CoBiF system, the area under the curve which was used to assess the predictability of mortality was 0.887. External validation results were also promising (area under the curve: 0.709). Conclusions: The presence of comorbidity bilateral pneumothorax and fever on presentation are significantly associated with poor prognosis in COVID-19 patients with spontaneous pneumothorax/pneumomediastinum. The CoBiF score can predict mortality in clinical settings as well as simplify the identification and appropriate management of patients at high risk.
From Virtual Reality to Regenerative Virtual Therapy: Some Insights from a Systematic Review Exploring Inner Body Perception in Anorexia and Bulimia Nervosa
Authors: Malighetti Clelia , Sansoni Maria , Gaudio Santino , Matamala-Gomez Marta , Di Lernia Daniele , Serino Silvia , Riva Giuseppe
Published: December 2022

Despite advances in our understanding of the behavioral and molecular factors that underlie the onset and maintenance of Eating Disorders (EDs), it is still necessary to optimize treatment strategies and establish their efficacy. In this context, over the past 25 years, Virtual Reality (VR) has provided creative treatments for a variety of ED symptoms, including body dissatisfaction, craving, and negative emotions. Recently, different researchers suggested that EDs may reflect a broader impairment in multisensory body integration, and a particular VR technique—VR body swapping—has been used to repair it, but with limited clinical results. In this paper, we use the results of a systematic review employing PRISMA guidelines that explore inner body perception in EDs (21 studies included), with the ultimate goal to analyze the features of multisensory impairment associated with this clinical condition and provide possible solutions. Deficits in interoception, proprioception, and vestibular signals were observed across Anorexia and Bulimia Nervosa, suggesting that: (a) alteration of inner body perception might be a crucial feature of EDs, even if further research is needed and; (b) VR, to be effective with these patients, has to simulate/modify both the external and the internal body. Following this outcome, we introduce a new therapeutic approach—Regenerative Virtual Therapy—that integrates VR with different technologies and clinical strategies to regenerate a faulty bodily experience by stimulating the multisensory brain mechanisms and promoting self-regenerative processes within the brain itself.
Left Heart Disease Phenotype in Elderly Patients with Pulmonary Arterial Hypertension: Insights from the Italian PATRIARCA Registry
Authors: Toma Matteo , Miceli Roberta , Bonsante Edoardo , Colombo Davide , Confalonieri Marco , Garascia Andrea , Ghio Stefano , Lattanzio Mariangela , Lombardi Carlo Maria , Paciocco Giuseppe , Piccinino Cristina , Rota Irene , Santolamazza Caterina , Scelsi Laura , Scuri Piermario , Stolfo Davide , Vincenzi Antonella , Volpiano Lorenzo , Vicenzi Marco , Ameri Pietro
Published: December 2022

Pulmonary arterial hypertension (PAH) in the elderly is often associated with left heart disease (LHD), prompting concerns about the use of pulmonary vasodilators. The PATRIARCA registry enrolled ≥70 year-old PAH or chronic thromboembolic pulmonary hypertension (CTEPH) patients at 11 Italian centers from 1 December 2019 through 15 September 2022. After excluding those with CTEPH, post-capillary PH at the diagnostic right heart catheterization (RHC), and/or incomplete data, 23 (33%) of a total of 69 subjects met the criteria proposed in the AMBITION trial to suspect LHD. Diabetes [9 (39%) vs. 6 (13%), p = 0.01] and chronic kidney disease [14 (61%) vs. 12 (26%), p = 0.003] were more common, and the last RHC pulmonary artery wedge pressure [14 ± 5 vs. 10 ± 3 mmHg, p < 0.001] was higher and pulmonary vascular resistance [5.56 ± 3.31 vs. 8.30 ± 4.80, p = 0.02] was lower in LHD than non-LHD patients. However, PAH therapy was similar, with 13 (57%) and 23 (50%) subjects, respectively, taking two oral drugs. PAH medication patterns remained comparable between LHD and non-LHD patients also when the former [37, 54%] were identified by atrial fibrillation and echocardiographic features of LHD, in addition to the AMBITION criteria. In this real-world snapshot, elderly PAH patients were treated with pulmonary vasodilators, including combinations, despite a remarkable prevalence of a LHD phenotype.
Reliability of 3D Stereophotogrammetry for Measuring Postoperative Facial Swelling
Authors: Buitenhuis Margje B. , Klijn Reinoud J. , Rosenberg Antoine J. W. P. , Speksnijder Caroline M.
Published: December 2022

This study aimed to determine the reliability of three-dimensional (3D) stereophotogrammetry as a measurement instrument for evaluating soft tissue changes in the head and neck area. Twelve patients received a bilateral sagittal split osteotomy (BSSO). Test and retest 3D photographs were captured within the first three postoperative weeks, and a reference 3D photograph was capture at three months postoperatively. Distance measurements, mean and root mean square of the distance map, and volume differences were obtained. Reliability of these parameters was assessed by intraclass correlation coefficients (ICCs), standard error of measurement (SEM), and smallest detectable change (SDC). All distance measurements had an ICC > 0.91, and the distance map parameters and volume differences showed ICCs > 0.89. The neck region presented the largest SEMs (5.09 mL) and SDC (14.1 mL) for the volume difference. In conclusion, 3D stereophotogrammetry is reliable for distance and volume measurements of soft tissues in patients after a BSSO advancement.
Associations between Fatty Acid Intake and Tension-Type Headache: A Cross-Sectional Study
Authors: Domínguez-Balmaseda Diego , Del-Blanco-Muñiz José Ángel , González-de-la-Flor Angel , García-Pérez-de-Sevilla Guillermo
Published: December 2022

Introduction: Patients with tension-type headache (TTH) are characterized by recurrent pain that can become disabling. Identifying the dietary triggers of headaches has led to defining dietary strategies to prevent this disease. In fact, excessive dietary intake of Omega-6 (ω-6) fatty acids, or an ω-6: ω3 ≥ 5 ratio, typical of Western diets, has been associated with a higher prevalence of headaches. The objectives of the present study were to compare dietary fatty acid intake between participants with and without chronic TTH and to investigate the association between dietary fatty acid intake, pain characteristics, and quality of life in patients with chronic TTH. Methods: An observational study was conducted, comparing healthy participants (n = 24) and participants diagnosed with chronic TTH for more than six months (n = 24). The variables analyzed were dietary fatty acid intake variables, the Headache Impact Test (HIT-6), and the characteristics of the headache episodes (intensity, frequency, and duration). Results: The TTH group reported a significantly higher intake of saturated fatty acids (SFAs) but similar intakes of monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), and ω-6: ω-3 ratio when compared to controls. Furthermore, in the TTH group, the Ω-6 fatty acid intake was associated with more intense headache episodes. In addition, the TTH group reported a significant impact of headaches on their activities of daily living according to the HIT-6. Conclusions: Higher intakes of SFAs and Ω-6 fatty acids were associated with more severe headache episodes in patients with TTH. Therefore, the characteristics of the diet, in particular the dietary fatty acid intake, should be considered when treating these patients.
Utilization of Antireflux Mucosectomy and Resection and Plication: A Novel Approach for the Management of Recurrent Gastroesophageal Reflux Disease after Prior Nissen Fundoplication or Transoral Incisionless Fundoplication
Authors: Randhawa Navkiran , Khalyfa Ahamed , Khan Mahnoor , Roebuck Christopher , Inam Mahnoor , Ayub Kamran
Published: December 2022

Background: Nissen Fundoplication (NF) and Transoral Incisionless Fundoplication (TIF) are established procedures for the treatment of gastroesophageal reflux disease (GERD). However, the surgically induced plication can loosen over time. This multicenter study aims to evaluate the safety and efficacy of Antireflux Mucosectomy (ARMS) and Resection and Plication (RAP) in symptomatic patients with prior NF or TIF that has become loose. Patients and methods: Eighteen patients were enrolled in the study. Ten had prior TIF, while eight had prior NF. Half of these patients had a Hill Grade 3 Valve while the other half had a Hill Grade 2 valve. Endoscopic submucosal dissection (ESD) was performed in six patients, while endoscopic mucosal resection (EMR) was performed in twelve patients. A follow-up endoscopy was performed at 4–12 weeks. Results: At follow-up, 11 patients had a Hill Grade 1 valve, and seven patients had a Hill Grade 2 valve. All patients had improvement in symptoms for up to 32 months. Conclusions: In this pilot study, ARMS/RAP appears to be an effective option in patients who had prior NF or TIF with recurrent GERD symptoms.
The Association of Preoperative PET-CT and Survival in Patients with Resectable Cervical Cancer
Authors: Su Chih-Hsiung , Chen Wan-Ming , Chen Ming-Chih , Shia Ben-Chang , Wu Szu-Yuan
Published: December 2022

Purpose: No randomized study with a long-term follow-up has investigated the effect of pretreatment 18-fluorodeoxyglucose positron emission tomography–computed tomography (18FDG-PET–CT) on the survival of patients with stage IB-IIA cervical cancer receiving curative surgery. Therefore, in this propensity score–matched, population-based cohort study, we investigated the effect of preoperative 18FDG-PET–CT on the survival outcomes of patients with potentially resectable cervical cancer. Patients and Methods: We included 2550 patients with stage IB-IIA cervical cancer receiving curative surgery with complete data on clinical stages. The patients were categorized into two 1:4 propensity, score–matched groups depending on whether they underwent pretreatment 18FDG-PET–CT, and their outcomes were compared. Results: We included 2030 and 520 patients with cervical cancer in the non-pretreatment and pretreatment PET–CT groups, respectively. Multivariable analyses revealed that the most prominent correlation between preoperative PET–CT and all-cause death was observed in the patients with stage IB–IIA cervical cancer receiving surgery (aHR [95% CI]: 1.16 [0.83–1.63]; p = 0.3752). Conclusions: Preoperative 18FDG-PET–CT was not associated with longer survival in the patients with clinical stage IB–IIA cervical cancer receiving curative surgery.
A New Role for Epidurography: A Simple Method for Assessing the Adequacy of Decompression during Percutaneous Plasma Disc Decompression
Authors: Gil Ho Young , Seo Wonseok , Choi Gyu Bin , Ha Eunji , Kim Taekwang , Ryu Jungyul , Kim Jae Hyung , Choi Jong Bum
Published: December 2022

Percutaneous plasma disc decompression (PPDD) is a minimally invasive treatment for discogenic low back pain and herniated disc-related symptoms. However, there are no known outcome predictive variables during the procedure. The purpose of this study was to evaluate and validate epidurography as an intra-procedure outcome predictor. We retrospectively enrolled 60 consecutive patients who did not respond to conventional treatments. In the next stage of treatment, PPDD was performed, and the epidurography was conducted before and after the PPDD. We analyzed the relationship between epidurographic improvement and the success rate. The Numerical Rating Scale and the Oswestry Disability Index were used to assess pain and functional capacity, respectively, before the procedure and 1 month after the procedure. The pain reduction and the success rate in the epidurographic improvement group were significantly higher than in the epidurographic non-improvement group. Both the Numerical Rating Scale and the Oswestry Disability Index scores were significantly reduced in both groups, but there was no significant difference in Oswestry Disability Index scores. This study’s results showed that PPDD is an effective treatment method. We also suggested that epidurography may be a potential outcome predictor for ensuring successful outcomes and determining the endpoint of the procedure.
Prognostic Impact of Severe Atrial Functional Tricuspid Regurgitation in Atrial Fibrillation Patients
Authors: Vîjan Ancuța Elena , Daha Ioana Cristina , Delcea Caterina , Bădilă Elisabeta , Dan Gheorghe-Andrei
Published: December 2022

Background and Aim: Atrial fibrillation (AF) is an epidemic disease with a significant global health impact. Atrial functional tricuspid regurgitation (AF-TR) is a more recently acknowledged complication of AF. The main purpose of this study was to determine the prognostic value of severe AF-TR in patients with AF, and its determinants. Methods: In this retrospective, observational study, we included AF patients admitted consecutively to a tertiary clinical hospital between January 2018 and February 2020, irrespective of cause of hospitalization. Patients with organic TR, significant pulmonary hypertension, left ventricular ejection fraction < 50%, those with implanted cardiac devices and those with in-hospital mortality were excluded. Severe TR was defined according to current guidelines. Median follow-up time was 34 (28–39) months. Primary endpoint was all-cause mortality. Results: We included 246 AF patients, with a mean age of 71.5 ± 9.4 years. 86.2% had AF-TR, while 8.1% had severe AF-TR. Mortality rate was 8.5%. Right atrial diameter (p = 0.005), systolic pulmonary artery pressure (sPAP) (p = 0.015) and NT-proBNP (p = 0.026) were independent predictors for the presence of severe valvular dysfunction. In multivariable survival analysis, severe AF-TR, was an independent predictor of all-cause mortality (HR 5.4, 95% CI 1.1–26.2, p = 0.035). Conclusion: Severe AF-TR was an independent predictor of mortality in AF patients, while mild/moderate AF-TR apparently had no impact on prognosis.
Atypical Response to Affective Touch in Children with Autism: Multi-Parametric Exploration of the Autonomic System
Authors: Bufo Maria Rosa , Guidotti Marco , Mofid Yassine , Malvy Joëlle , Bonnet-Brilhault Frédérique , Aguillon-Hernandez Nadia , Wardak Claire
Published: December 2022

This study aimed at evaluating the autonomic response to pleasant affective touch in children with Autism Spectrum Disorders (ASD) and age-matched typically developing (TD) peers, thanks to multiple autonomic nervous system (ANS) parameters and by contrasting CT (C-tactile fibers) high- vs. low-density territory stimulations. We measured pupil diameter, skin conductance, and heart rate during gentle stroking of two skin territories (CT high- and low-density, respectively, forearm and palm of the hand) in thirty 6–12-year-old TD children and twenty ASD children. TD children showed an increase in pupil diameter and skin conductance associated with a heart rate deceleration in response to tactile stimulations at the two locations. Only the pupil was influenced by the stimulated location, with a later dilation peak following CT low-density territory stimulation. Globally, ASD children exhibited reduced autonomic responses, as well as different ANS baseline values compared to TD children. These atypical ANS responses to pleasant touch in ASD children were not specific to CT-fiber stimulation. Overall, these results point towards both basal autonomic dysregulation and lower tactile autonomic evoked responses in ASD, possibly reflecting lower arousal and related to social disengagement.
Impact of Traditional and Non-Traditional Lipid Parameters on Outcomes after Intravenous Thrombolysis in Acute Ischemic Stroke
Authors: Ming Chua , Toh Emma M. S. , Yap Qai Ven , Yeo Leonard L. L. , Sia Ching-Hui , Ho Andrew F. W. , Chan Yiong Huak , Nastar Fathima Ashna , Lim Amanda Y. L. , Tan Benjamin Y. Q.
Published: December 2022

Contradicting evidence exists regarding the role of lipids in outcomes following intravenous (IV) thrombolysis with tissue plasminogen activator (tPA). Restricted cubic spline curves and adjusted logistic regression were used to evaluate associations of low-density lipoprotein cholesterol (LDL-C), non-high-density lipoprotein cholesterol (non-HDL-C), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and LDL-C/HDL-C ratio with poor functional outcome, symptomatic intracranial hemorrhage (SICH) and 90-day mortality, among 1004 acute ischemic stroke (AIS) patients who received IV tPA in a comprehensive stroke center. Quartile (Q) 1, Q2 and Q3 of HDL-C were associated with increased odds of poor functional outcome (adjusted odds ratio (adjOR) 1.66, 95% CI 1.06–2.60, p = 0.028, adjOR 1.63, 95% CI 1.05–2.53, p = 0.027, adjOR 1.56, 95% CI 1.01–2.44, p = 0.048) compared to Q4. Q2 and Q4 of non-HDL-C were associated with increased odds of SICH (adjOR 4.28, 95% CI 1.36–18.90, p = 0.025, adjOR 5.17, 95% CI 1.64–22.81, p = 0.011) compared to Q3. Q1 and Q2 of LDL-C was associated with increased odds of mortality (adjOR 2.57, 95% CI 1.27–5.57, p = 0.011 and adjOR 2.28, 95% CI 1.10–5.02, p = 0.032) compared to Q3. In AIS patients who received IV tPA, low LDL-C was associated with increased odds of mortality while HDL-C may be protective against poor functional outcome.
The Influence of Spatial and Temporal Scales on Fisheries Modeling—An Example of Sthenoteuthis oualaniensis in the Nansha Islands, South China Sea
Authors: Zhou Xingxing , Ma Shengwei , Cai Yancong , Yu Jie , Chen Zuozhi , Fan Jiangtao
Published: December 2022

The choice of spatial and temporal scales affects the performance of fisheries models and is particularly important in exploring the relationship between resource abundance and the marine environment. Traditional fishery models are constructed at a particular scale, and the results of the study hold only at that scale. Sthenoteuthis oualaniensis is one of the main target species of large-scale light falling-net fishing in the Nansha Islands in the South China Sea. We used the S. oualaniensis fishery in the Nansha Islands as an example to compare the performance of fisheries models for 12 spatial and temporal settings and to explore the relationship between the abundance of S. oualaniensis and the marine environment in the Nansha Islands under the optimal spatial and temporal settings. The results show that the spatial and temporal scale chosen in the construction of the fishery model is not as fine as possible in generalized additive models (GAMs) for abundance index-catch per unit effort (AI-CPUE)-based scenarios, and 0.5° with the season was the best spatial and temporal setting; meanwhile, in GAMs for AI-effort-based scenarios, 0.1° with the month was the best spatial and temporal setting. The distribution of S. oualaniensis resources in the Nansha Islands was characterized by significant seasonal variation, and the monthly center of gravity had a significant negative correlation with the Niño 3.4 index and the PDO index, with correlation coefficients of 100 and 1000, respectively. It is hypothesized that Pacific Decadal Oscillation and ENSO events affect the marine environment in the South China Sea by influencing the strength of the Kuroshio force and the degree of Kuroshio curvature, which in turn affects the distribution of S. oualaniensis in the Nansha Islands. The results help us to understand the influence of spatial and temporal scales on fisheries models and the environmental factors affecting the distribution of S. oualaniensis resources in the Nansha Islands. Thus, they provide a scientific basis for the sustainable development of S. oualaniensis fisheries in this region.
Biogeochemical Role of Algae in Aquatic Ecosystems: Basic Research and Applied Biotechnology
Authors: Lobus Nikolay V.
Published: December 2022

The term “algae” has no formal taxonomic position [...]
Effect of Flagellin Pre-Exposure on the Inflammatory and Antifungal Response of Bronchial Epithelial Cells to Fungal Pathogens
Authors: Bigot Jeanne , Ruffin Manon , Guitard Juliette , Vellaissamy Sandra , Thorez Sophie , Corvol Harriet , Guillot Loïc , Balloy Viviane , Hennequin Christophe
Published: December 2022

Bronchial epithelial cells (BEC) play a crucial role in innate immunity against inhaled fungi. Indeed, in response to microorganisms, BEC synthesize proinflammatory cytokines involved in the recruitment of neutrophils. We have recently shown that BEC exert antifungal activity against Aspergillus fumigatus by inhibiting filament growth. In the present study, we first analyzed the inflammatory and antifungal responses of BEC infected by several fungal species such as Aspergillus spp., Scedosporium apiospermum and Candida albicans, which are frequently isolated from the sputum of people with chronic pulmonary diseases. The airways of these patients, such as people with cystic fibrosis (pwCF), are mainly colonized by P. aeruginosa and secondary by fungal pathogens. We have previously demonstrated that BEC are capable of innate immune memory, allowing them to increase their inflammatory response against A. fumigatus following a previous contact with Pseudomonas aeruginosa flagellin. To identify the impact of bacteria exposure on BEC responses to other fungal infections, we extended the analysis of BEC innate immune memory to Aspergillus spp., Scedosporium apiospermum and Candida albicans infection. Our results show that BEC are able to recognize and respond to Aspergillus spp., S. apiospermum and C. albicans infection and that the modulation of BEC responses by pre-exposure to flagellin varies according to the fungal species encountered. Deepening our knowledge of the innate immune memory of BEC should open new therapeutic avenues to modulate the inflammatory response against polymicrobial infections observed in chronic pulmonary diseases such as CF.
Censorship, Pandemic, and the Field of Power: The Death and Revival of a Chinese War Epic
Authors: Liu Zhaoxi (Josie)
Published: December 2022

This case study examines the dramatic change of fortune of the Chinese war epic “The Eight Hundred”. The movie was censored in 2019 during China’s celebration of the country’s 70th anniversary but became the market-saving hero in the wake of the COVID-19 pandemic in 2020. Through the lens of Bourdieu’s theory of the field of cultural production, this study argues that the movie’s changing fate is essentially the change of its political, symbolic and economic capitals, under different field conditions. The subfield of commercial films in China is subject to the control of political and economic forces in the field of power, but is also becoming an economic power itself.
Personalized Medicine in Skull Base and Sinonasal Tumors
Authors: Mattavelli Davide , Bossi Paolo
Published: December 2022

Skull base and sinonasal tumors (SBSNTs) represent a considerable challenge for clinicians in view of their rarity, anatomical complexity of the site of origin, and great histological variety [...]
In Vitro Differentiation of Myoblast Cell Lines on Spider Silk Scaffolds in a Rotating Bioreactor for Vascular Tissue Engineering
Authors: Obed Doha , Dastagir Nadjib , Liebsch Christina , Bingoel Alperen S. , Strauss Sarah , Vogt Peter M. , Dastagir Khaled
Published: December 2022

Functional construction of tissue-engineered vessels as an alternative to autologous vascular grafts has been shown to be feasible, however the proliferation of seeded smooth-muscle cells remains a limiting factor. We employed a rotating bioreactor system to improve myoblast cell differentiation on a spider silk scaffold for tissue-engineered vessel construction. C2C12 myofibroblast cells were seeded on the surface of spider silk scaffold constructs and cultivated in a rotating bioreactor system with a continuous rotation speed (1 rpm). Cell function, cell growth and morphological structure and expression of biomarkers were analyzed using scanning electron microscopy, the LIVE/DEAD® assay, Western blot and quantitative real-time PCR analyses. A dense myofibroblast cell sheet could be developed which resembled native blood vessel muscular tissue in morphological structure and in function. Bioreactor perfusion positively affected cell morphology, and increased cell viability and cell differentiation. The expression of desmin, MYF5 and MEF2D surged as an indication of myoblast differentiation. Cell-seeded scaffolds showed a tear-down at 18 N when strained at a set speed (20 mm min−1). Spider silk scaffolds appear to offer a reliable basis for engineered vascular constructs and rotating bioreactor cultivation may be considered an effective alternative to complex bioreactor setups to improve cell viability and biology.
COVID-19 and Pregnancy: Have We Gotten through the Darkest Hour?
Authors: Favilli Alessandro , Laganà Antonio Simone , Chiantera Vito , Uccella Stefano , Gerli Sandro , Garzon Simone
Published: December 2022

On December 2019, a new Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) was isolated and identified in Wuhan (China) [...]
Sepsis as a Challenge for Personalized Medicine
Authors: Zahorec Roman , Průcha Miroslav
Published: December 2022

Sepsis is a clinical syndrome of systemic inflammation induced by infection, now defined as life-threatening organ dysfunction caused by a dysregulated immune response to infection [...]
Location Optimization of Offline Physical Stores Based on MNL Model under BOPS Omnichannel
Authors: Wan Peng , Zhang Jie , Liu Yiwei , Jiang Xiaoxuan
Published: December 2022

With the continuous upgrading of consumer demand and retail modes, more and more retailers are switching to an omnichannel retail mode. In order to study the location problem of offline physical stores of online retail enterprises that plan to implement the BOPS (Buy Online and Pickup in Store) omnichannel retail model, this paper considers consumers’ choice behavior under the omnichannel retail model; uses the MNL (Multinomial Logit) model to depict customers’ choice behavior between the online channel, BOPS channel, and offline physical channel; and constructs a location optimization model of offline physical stores with the goal of minimizing the enterprise’s cost. According to the characteristics of the model, an improved genetic algorithm was designed; the algorithm was improved on chromosome selection mode, crossover, and mutation rules. Finally, an example is calculated, and the physical store location scheme of a retail enterprise and the vehicle routing optimization scheme under the two-level distribution network are obtained, which verifies the effectiveness of the model and algorithm and provides a scientific reference for the physical store location decision of online retail enterprises planning to implement the BOPS omnichannel retail model. The impact of freight, return rate, and service level of physical stores on the location of offline physical stores is analyzed. The results show that in the process of expanding offline physical stores to implement the BOPS omnichannel retail model, online retail enterprises can reduce enterprise costs by improving the freight level and service level of the physical store. The higher the return rate of the online channel, the more necessary it is to expand offline physical stores, and the lower the enterprise cost. At the same time, management suggestions are put forward for the enterprise operation under the BOPS omnichannel retail mode.
Effect of Land Use-Land Cover Change on Soil Erosion and Sediment Yield in Muger Sub-Basin, Upper Blue Nile Basin, Ethiopia
Authors: Teshome Dawit Samuel , Moisa Mitiku Badasa , Gemeda Dessalegn Obsi , You Songcai
Published: December 2022

Land use and land cover (LULC) change have intense implications on soil resources, that requires research attention and global collaboration to take urgent actions. The present study attempted to analyze the impact of LULC change on soil erosion and sedimentation in Muger Sub-basin, southwestern parts of Ethiopia. Landsat TM 1986, Landsat ETM+ 2003, and Landsat OLI/TIRS 2020 are used for LULC analysis. We used rainfall erosivity, soil erodibility, slope length and steepness, cover management, and conservation practices to calculate soil erosion and sedimentation between 1986 and 2020. In this study, the integration of revised universal soil loss equation (RUSLE) model and geographic information system (GIS) are used to analyze the impact of LULC change on soil erosion and sedimentation. The average soil loss rate is about 53.2 ton/ha/year in 1986 and increased to 63.6, and 64 ton/ha/year in 2003, 2020, respectively. Result revealed that about 2707.7 ha (33%), 3124.5 ha (38.1%) and 3197.4 ha (38.9%) were exposed to severe erosion in 1986, 2003 and 2020, respectively. The mean sediment yields of the study area is estimated to 7.8 ton/ha/year in 1986, and later increased to 10, and 10.2 ton/ha/year in 2003, and 2020, respectively. Results revealed that the steep slope areas with crop land experience considerable soil loss, with mean soil loss increasing over all study period. Thus, urgent policy on the wise use of natural resources is essential not optional to reduce the current soil loss and sedimentation in Muger Sub-basin.
Anthropogenically Created Alpine Pastures as Landscape Resources for the Alpine Chamois Population in the Western Carpathians Mountain Range: Ďumbier Tatras Case Study
Authors: Hronček Pavel , Urban Peter , Gregorová Bohuslava , Čech Vladimír , Tometzová Dana
Published: December 2022

This study analysed the history of anthropogenically created alpine pastures from the 15th century to the present, as landscape resources for the chamois reintroduction in the second half of the 20th century in the Western Carpathians mountain arc (Slovakia), using the example of the Ďumbier Tatras (the second highest mountain range of the arc). Analysis and reconstructions were carried out on the basis of detailed archival and field research, which showed that grazing herds in the mountain peaks from the Middle Ages to the 20th century created anthropogenically suitable and sufficiently extensive grassy habitats for the chamois reintroduction and the survival of its population. The native chamois population became extinct in the Ďumbier Tatras at the end of the last ice age (about 10,000 years ago). However, anthropogenic deforestation has once again created suitable conditions for its distribution. In the 20th century, a new factor emerged, namely nature conservation and the proclamation of a national park, which meant the end of grazing in the alpine environment and the onset of succession. In the second half of the 20th century, modern tourism became another negative factor for the relocated Alpine chamois population, from the High Tatras back to its quasi-original environment. Tourism development was related to the construction of extensive infrastructure and superstructure in the chamois habitats in the Chopok and Ďumbier massifs. At present, therefore, the preservation of these ‘anthropogenic’ habitats requires active conservation and landscape management.
Minor Actinides Transmutation Performance in a Closed Th–U Cycle Based on Molten Chloride Salt Fast Reactor
Authors: He Liaoyuan , Chen Liang , Cui Yong , Xia Shaopeng , Zou Yang
Published: December 2022

The molten chloride salt fast reactor (MCFR) with a closed Th–U fuel cycle is receiving more and more attention due to its excellent performance, such as high solubility of actinides, superior breeding capacity, and good inherent safety. In this work, the neutronics performances for different minor actinides (MA) loadings and operation modes are analyzed and discussed based on an optimized MCFR. The results indicate that online continuous reprocessing can significantly increase the transmutation performance of MAs. In addition, MA loadings have an obvious effect on the neutronics characteristics of the MCFR, and it is helpful for improving the MA transmutation capability and 233U breeding performance, simultaneously. When MA = 5 mol%, the average annual MA transmutation mass and incineration mass can achieve about 53 kg and 13 kg, respectively, and the corresponding annual net production of 233U is 250 kg. When MA = 33.5 mol%, the annual MA transmutation mass and incineration mass can be about 310 kg and 77 kg, respectively, and the corresponding annual net production of 233U is 349 kg. However, when the MA loadings exceed 10%, the corresponding keff will exceed 1.1 for decades, even if only Th is continuously fed online. The results also indicate that the transmutation ratio (TR) and incineration ratio (IR) of MA increase and reach maximum values in the first decades for all the different MA loadings, which means MA may be fed into the fuel salt to improve its transmutation capability. Moreover, though MA loading will increase the level of radiotoxicity of the core in the early stage of burnup, the radiotoxicity of MA will drop rapidly after a brief rise during the operation. It can also be found that the temperature coefficient of reactivity (TCR) of all different MA loadings can be negative enough to maintain the safety of the MCFR during the whole operation, although it decreases in the beginning of life (BOL) with the increasing MA loading. Furthermore, the evolution of an effective delayed neutron fraction (EDNF) is also researched and discussed, and the EDNF varies most significantly when loading MA = 35.5 mol%, with a range of 273 to 310 pcm over the entire 100 years of operation.
Hydrolyzed Polyacrylamide as an In Situ Assistant in the Nucleation and Growth of Gold Nanoparticles
Authors: M. Aguilar Nery , Perez-Aguilar Jose Manuel , González-Coronel Valeria J. , Martínez-Gutiérrez Hugo , Zayas Pérez Teresa , Soriano-Moro Guillermo , Sanchez-Gaytan Brenda L.
Published: December 2022

The modulation of nanoparticles’ size, shape, and dispersion by polymers has attracted particular attention in different fields. Nevertheless, there is a lack of information regarding the use of charged macromolecules as assistants in the nanostructures’ nucleation and growth processes. Prompted by this, the in situ synthesis of gold nanoparticles (AuNPs) aided by hydrolyzed polyacrylamides (HPAM), with different chemical structures, was developed. In contrast to the conventional synthesis of nanostructures assisted by polyacrylamide, here, the polymerization, hydrolysis, and nanostructure formation processes were carried out simultaneously in the same milieu. Likewise, the growing chains acted as a template for the nanoparticles’ growth, so their conformations and chemical structure, especially the amount of charges along the chain, played an important role in the AuNPs’ morphology, size, and some of the final composite features. The nanocomposite was thoroughly characterized with appropriate techniques, including ATR–FTIR, GPC, UV–Vis, and SEM.
Selective Laser Melting (SLM) Additively Manufactured CoCrFeNiMn High-Entropy Alloy: Process Optimization, Microscale Mechanical Mechanism, and High-Cycle Fatigue Behavior
Authors: Zhang Jianrui , Yan Yabin , Li Bo
Published: December 2022

The equiatomic CoCrFeNiMn high-entropy alloy (HEA) possesses excellent properties including exceptional strength–ductility synergy, high corrosion resistance, and good thermal stability. Selective laser melting (SLM) additive manufacturing facilitates the convenient fabrication of the CoCrFeNiMn HEA parts with complex geometries. Here, the SLM process optimization was conducted to achieve a high relative density of as-built CoCrFeNiMn HEA bulks. The mechanisms of process-induced defects and process control were elucidated. The microscale mechanical behaviors were analyzed through in situ scanning electron microscopy observation during the compression tests on micro-pillars of the as-built HEA. The stress–strain characteristics by repeated slip and mechanism of “dislocation avalanche” during the compression of micro-pillars were discussed. The high-cycle fatigue tests of the as-built HEA were performed. It was found that a large number of nano-twins were induced by the fatigue, causing a non-negligible cycle softening phenomenon. The effects of promoted ductility due to the fatigue-induced nano-twins were illustrated. This work has some significance for the engineering application of the SLM additively manufactured CoCrFeNiMn HEA parts.