bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Habitat Disturbance on the Composition of Soil Nematode Functional Groups Associated with a Tropical Herb: Heliconia collinsiana
Authors: Benítez-Malvido Julieta , Jaramillo-López Pablo Fabián , Lobato-García Juan Manuel , Siliceo-Cantero Héctor Hugo , Mora-Ardila Francisco , Lombera Rafael
Published: October 2022

Habitat loss affects the nature of biotic interactions in all ecosystems and at all levels of the food web. Nevertheless, little attention has been given to soil nematodes in tropical habitats despite their important role in ecosystem functioning worldwide. Here, we analyzed the influence of anthropogenic habitat disturbance on the absolute and relative density and composition (i.e., trophic guilds) of soil nematode communities associated with the rhizosphere of the tropical herb Heliconia collinsiana in continuous mature forests and human-induced secondary forests. We compared nematode densities based on the following feeding guilds: bacterivores, fungivores, herbivores and predators. Thereafter, we classified herbivorous nematodes into genera and described soil properties in both habitat types including pH, electrical conductivity, and organic matter content. Herbivores were significantly the most abundant feeding guild for both habitats represented by Criconemella spp., Helicotylenchus spp., and Meloidogyne spp., which showed no significant differences in density between habitats. Relative but not absolute nematode density differed between habitats, with fungivore nematodes being significantly lower in secondary forests. No significant differences in soil properties were detected. Overall, our results suggest that forest disturbance affects the nematode community associated with the rhizosphere of H. collinsiana which may affect forest succession and the dynamics of the soil biota. Our study contributes to the understanding of biotic interactions in conserved and disturbed tropical habitats.
Risk Screening and Distribution of the Invasive Amphipod Dikerogammarus villosus (Sowinsky, 1894) in the River Adda (Northern Italy)
Authors: Paganelli Daniele , Cianci Maria Angela , Marchini Agnese
Published: October 2022

In 2016, Dikerogammarus villosus (Sowinsky, 1894) (Crustacea, Amphipoda) was recorded for the first time in the River Adda, one of the main tributaries of the major Italian river, river Po. Here we investigate its distribution, population density and size classes distribution in the main course of the river, in the territory of the South Adda Regional Park. Furthermore, we defined its level of invasiveness using two different risk screening methods, in order to obtain a more comprehensive evaluation of its potential impacts: AS-ISK (Aquatic Species Invasiveness Screening Kit) and Harmonia+. Finally, we compared the resulting invasiveness level with the output of the GISS (Generic Impact Scoring System) method, also used by IUCN. Our data confirm that the invasive amphipod is well-established in the downstream part of the River Adda, and its distribution seems related to hydrodynamism. Interestingly, its level of invasiveness was evaluated as low by Harmonia+ and high by AS-ISK: this discrepancy is due to different evaluations of environmental and socio-economic impacts of a non-native species carried out by the two methods. Finally, we propose some possible actions to reduce the spreading rate of this invasive amphipod in this area.
Current and Future Distribution Modeling of Socotra Cormorants Using MaxEnt
Authors: Jaradat Areej , Ksiksi Taoufik , Muzaffar Sabir Bin
Published: October 2022

The Socotra Cormorant (Phalacrocorax nigrogularis) is a regionally endemic seabird that is vulnerable due to human disturbance and habitat degradation. This study aimed to predict the potential current and future marine distribution of the species under different climate change scenarios using environmental variables affecting distribution using MaxEnt. Occurrence data were collected over several years using satellite tagged adults in the Arabian Gulf. The current model showed large areas of high suitability, mainly in the Arabian Gulf and in the Red Sea, where 31,300 km2 or 48% of total highly suitable areas existed. These areas are currently not utilized by the species. The future model predicted a sharp decline in suitable areas with 73% loss under the SSP5-8.5 climate change scenario of 2050 (extreme scenario). Nevertheless, the Red Sea is predicted to still hold considerable moderately suitable areas. Suitable areas increased around the Socotra archipelago. The model did not include biological variables due to lack of fish distribution data. Two variables, namely, mixed layer thickness and sea floor depth, explained most of the species’ distribution. These variables significantly influence nutrient cycling and forage fish distribution patterns, which in turn influence seabird distributions. Thus, the model could be useful in predicting the distribution of Socotra cormorants. However, the model outcomes should be interpreted with caution as potential areas of future expansion of the species to be further tested and validated. Conserving these areas as a precaution might encourage the Socotra Cormorant to colonize the region and persist in the future under the most extreme climate change scenarios, given that small forage fish that are eaten by the species are abundant in the predicted areas outside of the Arabian Gulf.
Conservation of Biocultural Diversity in the Huasteca Potosina Region, Mexico
Authors: Pensado-Leglise Mario del Roble , Luna-Vargas Salvador , Bustamante-Ramírez Hilda Angélica
Published: October 2022

The Huasteca Potosina region has a relevant landscape heritage of biocultural diversity, due to high biological diversity and the presence of the Teenek (Huastec Mayan), Nahua, and Xi’iuy (Pame) ethnic groups. The object of this study is to analyze, among the different cultural groups of the region, how the performances of the relevant Socioecological Systems (SESs) influence the conservation of biocultural diversity. Quantitative approaches are used to determine the expected trends of indices (Informant Consensus Factor, ICF; Cultural Importance Index, CII; Shannon–Wiener Biodiversity Index, SWI) commonly used in the ethnobotanical field. Data of the main domestic forest species used by the groups mentioned above were collected in 2021. We analyzed the SES profile for each of the ethnic groups and a mestizo group, as well as their relationship with the biome they mainly inhabit and the domestic functions fulfilled by the ethnobotanical species. As a result, we found that the low deciduous forest and the sub-evergreen tropical forest biomes, which co-evolved mainly with the Nahua and the Teenek SESs, present higher diversity and effective use of species so that offer better chances for conserving the landscape heritage of biocultural diversity. Otherwise, the results also show the critical nature regarding the biomes inhabited by the Pame and the mestizo’s SESs.
Odonata from Iberá Wetland System (Corrientes, Argentina) Are Regional Biogeographic Schemes Useful to Assess Odonata Biodiversity and Its Conservation?
Authors: del Palacio Alejandro , Lozano Federico , Ramos Lia S. , Navarro María de las Mercedes , Muzón Javier
Published: October 2022

Regionalization schemes reflect different macroscale distribution patterns and show large areas characterized by a common natural history, resulting in similar associations of biotic and abiotic features. Freshwater biota and terrestrial biota do not respond in the same way to environmental variables. The Iberá Depression, one of the largest wetlands in South America, is recognized in many schemes either as a functional unit or as an area with an ecotonal character. We used the distributional data of 128 species of Odonata, from a total of 103 collection sites from Corrientes and Misiones provinces, to test if Iberá functions as an ecological and functional unit, based on the Odonata distribution patterns. In addition, we tested if their distribution patterns fit into the most widespread regionalization schemes (hydrological basins, biogeographical provinces and ecoregions) used in Argentina. The Iberá Depression was not recovered as a functional unit; its sub-basins are more related to external basins than to each other. Neither the ecoregion nor the biogeographical schemes are suitable to explain the distribution patterns of the Odonata. The Odonata seem to respond to the availability of particular wetlands (e.g., ponds, streams, rivers, swamps, etc.), or to specific physical characteristics, such as the type of sediment, the availability of oxygen, etc., instead of to biogeographical or ecoregional schemes.
One Tree at a Time: Restoring Landscape Connectivity through Silvopastoral Systems in Transformed Amazon Landscapes
Authors: Argote Karolina , Rodríguez-Sánchez Beatriz , Quintero Marcela , Francesconi Wendy
Published: October 2022

Due to the continued expansion of pastures and illicit crops, the Andes-Amazon foothills in Colombia are one of most threatened biodiversity hotspots in the country. Halting and restoring the connectivity of the landscapes transformed over the last 40 years and now dominated by extensive cattle ranching practices, represents a challenge. Silvopastoral systems (SPSs) have been proposed as a strategy to help conserve the biodiversity by improving landscape connectivity. However, understanding the contributions of SPSs to biodiversity conservation still requires additional research. At the farm scale (here called farmscape), we compared different landscape fragmentation and connectivity metrics under two SPS conditions (with and without). Overall, the adoption of SPSs increased the probability of connectivity (PC) index in all cases. However, the contributions of SPSs to landscape connectivity were not linear. Greater PC increases were observed in highly degraded farmscapes (ΔPc = 284) compared to farmscapes containing patches that were better connected and had larger habitat areas (ΔPc = 6). These variables could play a fundamental role in enhancing the landscape connectivity through restoration activities that seek to improve biodiversity conservation. Even if they are relatively small and scattered, in highly degraded cattle ranching systems, SPSs could significantly improve the landscape connectivity, which in turn could improve wildlife conservation.
Consumer Perceptions towards Unsolicited Advertisements on Social Media
Authors: Romano Rhoderick , Han Jeongsoo
Published: October 2022

The practice of unsolicited advertisements on social media has grown prevalent. This data article presents 837 US-based social media users’ consumer perceptions of such advertisements. Understanding how consumers perceive unsolicited advertising is vital to developing effective digital marketing strategies. Data collection was via an online survey adopting multi-measurement items from extant studies for reliability and validity. The data showed high internal consistency with Cronbach’s alpha testing, and confirmatory factor analysis (CFA) found the measurement model valid. Goodness-of-fit indices showed a good fit with the data. Finally, convergent and discriminant validity was confirmed using the composite reliability, average variance extracted (AVE), and correlations among constructs. Further research may utilise the data using inferential analysis techniques to add to our understanding of consumer perceptions of unsolicited advertising on social media.
Design for Manufacturing, Assembly, and Reliability: An Integrated Framework for Product Redesign and Innovation
Authors: Juniani Anda Iviana , Singgih Moses Laksono , Karningsih Putu Dana
Published: October 2022

A product redesign strategy can effectively shorten design lead time and reduce the manufacturing cost of innovative development for the manufacturing industry to stay competitive. Identification of function components is the basis of product redesign. Existing practices to identify the critical component and customer requirements are considered while complaint and historical failure data, crucial for improving product reliability, are frequently ignored. The objective of the article is to develop an integrated framework of product redesign and innovation considering customer requirements and product failure modes. The novel framework integrates the design for manufacturing and assembly (DFMA) and design for reliability (DFR) approaches, as well as finite element analysis to address the cost reduction of product redesign at early-stage, reliability improvement, and higher customer satisfaction. The proposed integrated framework is validated using an example of an Indonesian SME’s cooking stove. The redesigned cooking stove showed substantial improvements with 5.46% cost reductions, 20.32% design efficiency, and an 52.81% safety factor.
TACRP: Traffic-Aware Clustering-Based Routing Protocol for Vehicular Ad-Hoc Networks
Authors: Habelalmateen Mohammed , Ahmed Ahmed , Abbas Ali , Rashid Sami
Published: October 2022

On account of the highly dynamic topology of vehicular networks, network congestion and energy utilization are greatly increased, which directly affects the performance of VANETs. So, managing traffic and reducing energy consumption in the network becomes a challenging task in such huge mobility-based VANET networks. Thus, in this paper a new traffic and cluster-based network method is introduced, namely, Traffic-Aware Clustering based Routing Protocol (TACRP). The main aim of the approach is to improve traffic management in the network as well as to reduce energy consumption in it. In the constructed network, a Traffic Management Unit (TMU) is introduced to control the entire network traffic with the help of RSUs. Vehicles with similar speed and direction are grouped into a cluster to increase the network stability and help to reduce the energy consumption of the network. The clustering model provides principles associated with vehicles leaving the clusters, joining the clusters, cluster updates and inter-cluster communication, which makes the network more stable and reliable. For instance, in the proposed work the CH selection is based on centralization, weight, distance, and energy calculation. Such network settings facilitate successfully clustering of vehicles on the road. Simulation experimental analysis showed that the proposed TACRP routing protocol achieved better results in terms of energy efficiency, throughput, packet delivery ratio, and end to end delay of the network when compared with earlier methods, such as ECHS and NRHCS.
Prospects of Triangular Modular Structures for Roadside Service Buildings
Authors: Samoilov Konstantin , Kuspangaliyev Bolat , Sadvokasova Gaukhar , Kuanyshbekov Nurlytan
Published: October 2022

The need for a relatively quick solution to the problem of providing highways with roadside service facilities necessitates the development of a series of appropriate standard projects. To increase the efficiency of these series, it is advisable to carry out the interconnection of space-planning solutions based on a particular module. Taking into account the variety of planning and landscape characteristics of the sites for the placement of objects of the mainline service, it seems advisable to choose as a module not a square or rectangular, but a triangular configuration, which allows in most cases to harmoniously block the modules. The proposed roof module in the form of a “regular” triangle facing the tetrahedron has a structural basis in the form of a single-tier rod spatial plate. The principal space-planning solutions of all four dozen objects from the approved nomenclature of the mainline service performed in the process of analyzing the possibilities show the real possibility of solving the development tasks on the basis of this system. The use of the proposed modular system makes it possible to successfully solve a number of tasks to reduce the harmful impact on the environment and effectively use renewable energy sources. The work is devoted specifically to the field of design.
Improved Local Scale Generic Cycle Model for Aerothermodynamic Simulations of Gas Turbine Engines for Propulsion
Authors: Gurrola Arrieta Manuel de Jesús , Botez Ruxandra Mihaela
Published: October 2022

A local scale Aerothermodynamic Generic Cycle Model (AGCM) is proposed. The AGCM accounts for several improvements not considered in similar models, such as compressor bleed extraction for aircraft Environmental Control System (ECS), parasitic shaft power extraction, and the enthalpy of the fuel entering the combustor. The AGCM is intended for steady-state Design Point (DP) and Off-Design (OD) performance analyses. The underlying physics is presented for the DP model. The turbomachinery component maps scaling and the system of nonlinear equations necessary to define the OD model are thoroughly discussed. The AGCM is compared with an equivalent model developed in the Numerical Propulsion System Simulation (NPSS). The comparisons were performed considering a DP envisioned to approximate a General Electric CF34-8C5B1 engine. The average errors found in these comparisons for the Specific Fuel Consumption (SFC) and net thrust were −0.111% and 0.193%, respectively. Finally, the predictions of the absolute levels of performance intended for the -8C5B1 engine are compared with empirical correlations derived from a comprehensive turbofan engine database. It was found that the predictions of the AGCM are in agreement with the empirical correlations; the errors found in SFC and net thrust at cruise flight condition were −0.43% and 2.06%, respectively.
Neurocognitive Disorders in Post and Long Covid Patients: Preliminary Data, Gender Differences and New Diabetes Diagnosis
Authors: Mezzatesta Concetta , Bazzano Sara , Gesualdo Rosa , Marchese Simone , Savona Maria Luisa , Reyes Mario Tambone , Provenzano Vincenzo
Published: October 2022

The research is based on a clinical observation of the neurological and neuro-cognitive status of 300 patients, belonging to the Partinico Hospital and the Post-Long Covid clinic, which had contracted the SARS-CoV-2 virus in the period between April 2021 and May 2022. In this paper, we present the analysis of the first 100 patients subjected to a neurocognitive screening protocol. The procedure consists of tests that examine the mechanism of different brain domains to check for the presence of cognitive deficits that arose after the negativization of the viral infection. Through a neurocognitive protocol, the research aims to investigate different brain areas and mental functioning. This allowed us to raise the possibility that the presence of cognitive alterations may be related to the evidence of point-like brain alterations (from the cortex to the trunk) visible through neuroimaging techniques. In the article, we highlight the hypothesis that SARS-covid 2, as stated in recently published studies, can produce an alteration of executive functions such as to configure a real dysexecutive syndrome. This research evaluates the symptomatic gender variability within the sample, the presence of important differences in the affective state, and provides a first observation of the impact of SARS-CoV-2 in diabetic pathology as well.
Psychiatric Illness or Immune Dysfunction—Brain Perfusion Imaging Providing the Answer in a Case of Anti-NMDAR Encephalitis
Authors: Šiško Markoš Ines , Blažeković Ivan , Peitl Vjekoslav , Jukić Tomislav , Supanc Višnja , Karlović Dalibor , Fröbe Ana
Published: October 2022

Background: We investigated the potential use of SPECT quantification in addition to qualitative brain perfusion analysis for the detection of anti-NMDAR encephalitis. The question is how to normalize brain activity to be able to quantitatively detect perfusion patterns. Usually, brain activity is normalized to a structure considered unaffected by the disease. Methods: Brain [99mTc]-HMPAO SPECT was performed as a method to detect brain perfusion patterns. The patterns of abnormal brain perfusion cannot always be reliably and qualitatively assessed when dealing with rare diseases. Recent advances in SPECT quantification using commercial software have enabled more objective and detailed analysis of brain perfusion. The cerebellum and whole brain were used as the normalization structures and were compared with visual analysis. Results: The quantification analysis performed with whole brain normalization confirmed right parietal lobe hypoperfusion while also detecting statistically significant left-to-right perfusion differences between the temporal lobe and thalamus. Whole brain normalization further described bilateral frontal lobe hyperperfusion, predominantly of the left lobe, and was in accordance with visual analysis. Conclusion: SPECT quantitative brain perfusion analysis, using the whole brain as the normalization structure rather than the cerebellum, in this case, improved confidence in the visual detection of anti-NMDAR encephalitis and provided unexpected solutions to atypical psychiatric dilemmas.
Systemic Inflammatory Biomarkers and Chest CT Findings as Predictors of Acute Limb Ischemia Risk, Intensive Care Unit Admission, and Mortality in COVID-19 Patients
Authors: Arbănași Emil Marian , Halmaciu Ioana , Kaller Réka , Mureșan Adrian Vasile , Arbănași Eliza Mihaela , Suciu Bogdan Andrei , Coșarcă Cătălin Mircea , Cojocaru Ioana Iulia , Melinte Razvan Marian , Russu Eliza
Published: October 2022

Background: Numerous tools, including inflammatory biomarkers and lung injury severity scores, have been evaluated as predictors of thromboembolic events and the requirement for intensive therapy in COVID-19 patients. This study aims to verify the predictive role of inflammatory biomarkers [monocyte to lymphocyte ratio (MLR), neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR), systemic inflammatory index (SII), Systemic Inflammation Response Index (SIRI), and Aggregate Index of Systemic Inflammation (AISI)] and the CT Severity Score in acute limb ischemia (ALI) risk, intensive unit care (ICU) admission, and mortality in COVID-19 patients.; Methods: The present study was designed as an observational, analytical, retrospective cohort study and included all patients older than 18 years of age with a diagnosis of COVID-19 infection, confirmed through real time-polymerase chain reaction (RT-PCR), and admitted to the County Emergency Clinical Hospital of Targu-Mureș, Romania, and Modular Intensive Care Unit of UMFST “George Emil Palade” of Targu Mures, Romania between January 2020 and December 2021. Results: Non-Survivors and “ALI” patients were associated with higher incidence of cardiovascular disease [atrial fibrillation (AF) p = 0.0006 and p = 0.0001; peripheral arterial disease (PAD) p = 0.006 and p < 0.0001], and higher pulmonary parenchyma involvement (p < 0.0001). Multivariate analysis showed a high baseline value for MLR, NLR, PLR, SII, SIRI, AISI, and the CT Severity Score independent predictor of adverse outcomes for all recruited patients (all p < 0.0001). Moreover, the presence of AF and PAD was an independent predictor of ALI risk and mortality. Conclusions: According to our findings, higher MLR, NLR, PLR, SII, SIRI, AISI, and CT Severity Score values at admission strongly predict ALI risk, ICU admission, and mortality. Moreover, patients with AF and PAD had highly predicted ALI risk and mortality but no ICU admission.
Point-of-Care Echocardiographic Characteristics of COVID-19 Patients with Pulmonary Embolism
Authors: Klasnja Slobodan , Manojlovic Andrea , Popadic Viseslav , Ivankovic Tatjana , Ninkovic Nebojsa , Rajovic Nina , Popovic Maja , Nikolic Novica , Brajkovic Milica , Radojevic Aleksandra , Lasica Ratko , Rajsic Sasa , Todorovic Zoran , Brankovic Marija , Radonjic Tijana , Memon Lidija , Mrda Davor , Milic Natasa , Zdravkovic Marija
Published: October 2022

Introduction: Thrombotic complications, such as pulmonary embolism, are common in COVID-19 patients. Point-of-care ultrasound is a highly recommended tool for orientation in critically ill patients with suspected or confirmed complications. Methods: An observational study was conducted on 32 consecutive patients with confirmed pulmonary embolism and COVID-19 infection treated in the Intensive Care Unit of the University Hospital Medical Center “Bežanijska kosa”, Belgrade, Serbia, between April 2021 and March 2022. Predictors of the need for oxygen support were determined, while point-of-care echocardiographic parameters and various anamnestic, laboratory, and clinically significant parameters were correlated with the Pulmonary Embolism Severity Index (PESI) score. Results: More than two-thirds of patients in our study had PE symptoms present at hospital admission (68.8%). The majority of patients had segmental pulmonary embolism (48.4%), with high to very high PESI score values in 31.3% of patients. Pneumonia was present in 68.8% of the study population. The PESI score was negatively correlated with diastolic blood pressure and SaO2 at the time of PE diagnosis, LV ejection fraction, and PVAT. A positive correlation was found between the PESI score, maximum CRP, and D-dimer at the time of PTE diagnosis. A larger right ventricular diameter was associated with a greater need for oxygen support. Conclusion: Point-of-care echocardiography is a valuable tool for the risk assessment of COVID-19 patients with pulmonary embolism. Right ventricular size stood out as a significant marker of disease severity.
Using 18F-FDG-PET/CT Metrics to Predict Survival in Ra-Dio-Iodine Refractory Thyroid Cancers
Authors: Roy Malanie , Edet-Sanson Agathe , Lefebvre Hervé , Vera Pierre , Decazes Pierre
Published: October 2022

Radio-iodine refractory (RAI-R) differentiated thyroid cancer (DTC) is a rare disease with a poor prognosis and limited therapeutic resources. Therefore, identifying prognostic factors is essential in order to select patients who could benefit from an early start of treatment. The aim of this study is to identify positron emission tomography with 18F-fluorodeoxyglucose with integrated computed tomography (18F-FDG-PET/CT) parameters to predict overall survival (OS) in patients with RAI-R DTC. In this single-center retrospective study, we analyze the 18F-FDG-PET/CT parameters of 34 patients with RAI-R DTC between April 2007 and December 2019. The parameters collected are MTV, SUVmax and progression for each site of metastasis (neck, mediastinum, lungs, liver, bone) and total sites. ROC curves, Kaplan–Meier survival analysis curves, univariate and multivariate Cox analyses determine prognostic factors for 1-year and 5-year OS. The parameters for mediastinum, liver and total sites are significantly associated with worse 1-year and 5-year OS by both ROC curve analysis and Kaplan–Meier survival analysis. Univariate Cox analysis confirms significance of mediastinum SUVmax (HR 1.08; 95% CI [1.02–1.15]; p = 0.014) and total SUVmax (HR 1.06; 95% CI [1–1.12]; p = 0.042) for worse 1-year OS; of mediastinum SUVmax (HR 1.06; 95% CI [1.02–1.10]; p = 0.003), liver SUVmax (HR 1.04; 95% CI [1.01–1.08]; p = 0.02), liver MTV (HR 2.56; 95% CI [1.13–5.82]; p = 0.025), overall SUVmax (HR 1.05; 95% CI [1.02–1.08]; p = 0.001) and total MTV (HR 1.41; 95% CI [1.07–1.86]; p = 0.016) for worse 5-year OS. Multivariate Cox analysis confirms a significant association between liver MTV (HR 1.02; 95% CI [1–1.04]; p = 0.042) and decrease 1-year OS. In this study, we demonstrate that in RAI-R DTC, 18F-FDG-PET/CT parameters of the mediastinum, liver and overall tumor burden were prognostic factors of poor 1-year and 5-year OS. Identifying these criteria could allow early therapeutic intervention in order to improve patients’ survival.
High EZH2 Protein Expression Is a Poor Prognostic Predictor in IDH1 R132H-Negative Gliomas
Authors: Wong Yin Ping , Che Abdul Aziz Roziasyazni , Noor Aizuddin Azimatun , Mohd Saleh Muhamad Fakhri , Mohd Arshad Roslina , Tan Geok Chin
Published: October 2022

Accumulating data indicates that enhancer of zeste homology 2 (EZH2) and isocitrate dehydrogenase 1 (IDH1) are implicated in promoting tumourigenesis in a myriad of malignancies including gliomas. We aimed to determine the immunoexpression of EZH2 in gliomas and its correlation with clinicopathological variables. The prognostic value of the combined immunoexpression of EZH2 and IDH1 was further explored in a retrospective analysis involving 56 patients with histologically confirmed gliomas in Universiti Kebangsaan Malaysia Medical Centre from 2010 to 2016. The patients were then followed up for a period of five years. EZH2 and IDH1 R132H immunoexpressions were performed and analysed on respective tissue blocks. Five-year progression-free survival (PFS) and overall survival (OS) were estimated by Kaplan–Meier analysis. Univariate and multivariate Cox proportional hazard regression models were performed to evaluate the value of EZH2 as an independent factor for the prediction of PFS and OS. High EZH2 immunoexpression was demonstrated in 27 (48.2%) gliomas. High EZH2 expression was significantly correlated with older age (p = 0.003), higher tumour grade (p < 0.001), negative IDH1 R132H immunoexpression (p = 0.039), a poor 5-year PFS (mean = 9.7 months, p < 0.001) and 5-year OS (mean = 28.2 months, p = 0.007). In IDH1 R132H-negative gliomas, there was a trend toward shorter 5-year PFS (mean = 8.0 months, p = 0.001) and 5-year OS (mean = 28.7 months, p = 0.06) in gliomas demonstrating high EZH2 expression compared with those with low EZH2 expression. High EZH2 immunoexpression is an unfavourable independent prognostic predictor of poor survival in gliomas. EZH2 analysis might therefore be of clinical value for risk stratification, especially in patients with IDH1 R132H-negative gliomas.
Role of MRI-Derived Radiomics Features in Determining Degree of Tumor Differentiation of Hepatocellular Carcinoma
Authors: Ameli Sanaz , Venkatesh Bharath Ambale , Shaghaghi Mohammadreza , Ghadimi Maryam , Hazhirkarzar Bita , Rezvani Habibabadi Roya , Aliyari Ghasabeh Mounes , Khoshpouri Pegah , Pandey Ankur , Pandey Pallavi , Pan Li , Grimm Robert , Kamel Ihab R.
Published: October 2022

Background: To investigate radiomics ability in predicting hepatocellular carcinoma histological degree of differentiation by using volumetric MR imaging parameters. Methods: Volumetric venous enhancement and apparent diffusion coefficient were calculated on baseline MRI of 171 lesions. Ninety-five radiomics features were extracted, then random forest classification identified the performance of the texture features in classifying tumor degree of differentiation based on their histopathological features. The Gini index was used for split criterion, and the random forest was optimized to have a minimum of nine participants per leaf node. Predictor importance was estimated based on the minimal depth of the maximal subtree. Results: Out of 95 radiomics features, four top performers were apparent diffusion coefficient (ADC) features. The mean ADC and venous enhancement map alone had an overall error rate of 39.8%. The error decreased to 32.8% with the addition of the radiomics features in the multi-class model. The area under the receiver-operator curve (AUC) improved from 75.2% to 83.2% with the addition of the radiomics features for distinguishing well- from moderately/poorly differentiated HCCs in the multi-class model. Conclusions: The addition of radiomics-based texture analysis improved classification over that of ADC or venous enhancement values alone. Radiomics help us move closer to non-invasive histologic tumor grading of HCC.
SARS-CoV-2 Variants Monitoring Using Real-Time PCR
Authors: Esman Anna , Cherkashina Anna , Mironov Konstantin , Dubodelov Dmitry , Salamaikina Svetlana , Golubeva Anna , Gasanov Gasan , Khafizov Kamil , Petrova Natalya , Cherkashin Evgeniy , Akimkin Vasiliy
Published: October 2022

According to the temporary recommendations of the 2021 World Health Organization (WHO), in addition to whole-genome sequencing, laboratories in various countries can also screen for known mutations utilizing targeted RT-PCR-based mutation detection assays. The aim of this work was to generate a laboratory technique to differentiate the main circulating SARS-CoV-2 variants in 2021–2022, when a sharp increase in morbidity was observed with the appearance of the Omicron variant. Real-time PCR methodology is available for use in the majority of scientific and diagnostic institutions in Russia, which makes it possible to increase the coverage of monitoring of variants in the territories of all 85 regions in order to accumulate information for the Central Services and make epidemiological decisions. With the methodology developed by the Central Research Institute of Epidemiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (FSSCRP Human Wellbeing) (CRIE), more than 6000 biological samples have been typed, and 7% of samples with the Delta variant and 92% of samples with the Omicron variant have been identified as of 25 August 2022. Reagents for 140,000 definitions have been supplied to regional organizations.
Tensor-Based Learning for Detecting Abnormalities on Digital Mammograms
Authors: Tzortzis Ioannis N. , Davradou Agapi , Rallis Ioannis , Kaselimi Maria , Makantasis Konstantinos , Doulamis Anastasios , Doulamis Nikolaos
Published: October 2022

In this study, we propose a tensor-based learning model to efficiently detect abnormalities on digital mammograms. Due to the fact that the availability of medical data is limited and often restricted by GDPR (general data protection regulation) compliance, the need for more sophisticated and less data-hungry approaches is urgent. Accordingly, our proposed artificial intelligence framework utilizes the canonical polyadic decomposition to decrease the trainable parameters of the wrapped Rank-R FNN model, leading to efficient learning using small amounts of data. Our model was evaluated on the open source digital mammographic database INBreast and compared with state-of-the-art models in this domain. The experimental results show that the proposed solution performs well in comparison with the other deep learning models, such as AlexNet and SqueezeNet, achieving 90% ± 4% accuracy and an F1 score of 84% ± 5%. Additionally, our framework tends to attain more robust performance with small numbers of data and is computationally lighter for inference purposes, due to the small number of trainable parameters.
Classification of Vascular Hotspots and Micro-Vessel Flow Velocity Waveforms in Low-Grade Squamous Intraepithelial Lesions and HPV Condyloma of the Cervix
Authors: Wu Yi-Cheng , Chen Ching-Hsuan , Ko Yi-Li , Yuan Chiou-Chung , Wang Peng-Hui , Chu Woei-Chyn
Published: October 2022

To assess hotspot micro-vessel flow velocity waveforms in human papillomavirus (HPV) cervical infections using transvaginal power Doppler ultrasound (TV-PDU) and to explore the associations of these sonographic parameters with HPV condyloma and low-grade squamous intraepithelial lesions (LSIL) of the cervix. A total of 39 patients with cervical HPV infections with abnormal cytology and colposcopy results (26 cases of LSIL; 13 cases of HPV condyloma) were enrolled to assess the vascular classification of the cervix and micro-vessel flow velocity using TV-PDU before treatment; 40 individuals with a pathologically normal cervix were used as the control group; seven parameters were measured, including vascular grading classification (Class I, Class II, and Class III), lowest pulsatility index (PI), resistance index (RI), peak systolic velocity (PS), end-diastolic velocity (ED), time average maximum velocity (TAMV), and the vascular index (VI = PS/ED). According to vascular classification, most LSILs were class I (69.2%, 18/26), followed by class II (26.9%, 7/26) and class III (3.8%, 1/26). Most HPV condylomas were class I (92.3%, 12/13), and one was class II (7.7%, 1/13). PI, RI, VI (p < 0.0001), and the PSs (p < 0.05) were significantly lower in these cases than in the controls. The ED and TAMV were not significantly different between the patients and controls (p = 0.4985 and p = 0.1564). No sonographic parameter was significantly different between LSIL and HPV condyloma. The mean PI, RI, and VI were significantly lower in LSIL than in the controls. For HPV condyloma, a PI of 1.07 had an 84.6% sensitivity, 85.0% specificity, and an AUC of 88.8%; for LSIL, a PI of 1.08 had a 100% sensitivity, 85% specificity, and an AUC of 94.2%; for HPV infection (HPV condyloma + LSIL), a PI of 1.08 had a 94.9% sensitivity, 85% specificity, and an AUC of 92.4%. Hotspot vascular classification and micro-vessel flow velocity waveforms may provide a potential practical method for the auxiliary diagnosis of cervical HPV infection. The PI may represent a valuable index for distinguishing the micro-vessel flow velocity waveforms in LSIL and HPV condyloma. Since the case numbers were limited in the current study, further validation is needed.
Technical Outcome, Clinical Success, and Complications of Low-Milliampere Computed Tomography Fluoroscopy-Guided Drainage of Lymphoceles Following Radical Prostatectomy with Pelvic Lymph Node Dissection
Authors: D’Anastasi Melvin , Ebenberger Simone , Alghamdi Abdulmajeed , Helck Andreas , Herlemann Annika , Stief Christian , Khoder Wael , Trumm Christoph G. , Stahl Robert
Published: October 2022

To evaluate the technical outcome, clinical success, and safety of low-milliampere CT fluoroscopy (CTF)-guided percutaneous drain (PD) placement in patients with lymphoceles following radical prostatectomy (RP) with pelvic lymph node dissection (LND). This retrospective analysis comprised 65 patients with PD placement in lymphoceles following RP under low-milliampere CTF guidance. Technical and clinical success were evaluated. Complications within a 30-day time interval associated with CTF-guided PD placement were classified according to SIR. Patient radiation exposure was quantified using dose-length products (DLP) of the pre-interventional planning CT scan (DLPpre), of the sum of intra-interventional CT fluoroscopic acquisitions (DLPintra) and of the post-interventional control CT scan (DLPpost). Eighty-nine lymphoceles were detected. Seventy-seven CT-guided interventions were performed, with a total of 92 inserted drains. CTF-guided lymphocele drainage was technically successful in 100% of cases. For all symptomatic patients, improvement in symptoms was reported within 48 h after intervention. Time course of C-reactive protein and Leucocytes within 30 days revealed a statistically significant (p < 0.0001) decrease. Median DLPpre, DLPintra and DLPpost were 431 mGy*cm, 45 mGy*cm and 303 mGy*cm, respectively. Only one minor complication (self-resolving haematoma over the bladder dome; SIR Grade 2) was observed. Low-milliampere CTF-guided drainage is a safe treatment option in patients with lymphoceles following RP with pelvic LND characterized by high technical and good clinical success rates, which provides rapid symptom relief and serves as definite treatment or as a bridging therapy prior to laparoscopic marsupialisation.
Measurement and Modeling of the Optical Properties of Adipose Tissue in the Terahertz Range: Aspects of Disease Diagnosis
Authors: Yanina Irina Y. , Nikolaev Viktor V. , Zakharova Olga A. , Borisov Alexei V. , Dvoretskiy Konstantin N. , Berezin Kirill V. , Kochubey Vyacheslav I. , Kistenev Yuri V. , Tuchin Valery V.
Published: October 2022

In this paper, the measurement and modeling of optical properties in the terahertz (THz) range of adipose tissue and its components with temperature changes were performed. Spectral measurements were made in the frequency range 0.25–1 THz. The structural models of main triglycerides of fatty acids are constructed using the B3LYP/6-31G(d) method and the Gaussian03, Revision B.03 program. The optical density (OD) of adipose tissue samples decreases as temperature increases, which can be associated mostly with the dehydration of the sample. Some inclusion of THz wave scattering suppression into the OD decrease can also be expected due to refractive index matching provided by free fatty acids released from adipocytes at thermally induced cell lipolysis. It was shown that the difference between the THz absorption spectra of water and fat makes it possible to estimate the water content in adipose tissue. The proposed model was verified on the basis of molecular modeling and a comparison with experimental data for terahertz spectra of adipose tissue during its heating. Knowing the exact percentage of free and bound water in adipose tissue can help diagnose and monitor diseases, such as diabetes, obesity, and cancer.
Acute Kidney Injury Predictors and Outcomes after Cardiac Surgery in Children with Congenital Heart Disease: An Observational Cohort Study
Authors: Kourelis Georgios , Kanakis Meletios , Samanidis George , Tzannis Kimon , Bobos Dimitrios , Kousi Theofili , Apostolopoulou Sotiria , Kakava Felicia , Kyriakoulis Konstantinos , Bounta Stavroula , Rammos Spyridon , Papagiannis John , Giannopoulos Nickolas , Orfanos Stylianos E. , Dimopoulos George
Published: October 2022

Acute Kidney Injury (AKI) commonly complicates cardiac surgery in children with congenital heart disease (CHD). In this study we assessed incidence, risk factors, and outcomes of postoperative AKI, while testing the hypothesis that, depending on the underlying diagnosis, there would be significant differences in AKI incidence among different diagnostic groups. We conducted an observational cohort study of children with CHD undergoing cardiac surgery in a single tertiary center between January 2019 and August 2021 (n = 362). Kidney Disease Improving Global Outcome (KDIGO) criteria were used to determine the incidence of postoperative AKI. Diagnosis was incorporated into multivariate models using an anatomic-based CHD classification system. Overall survival was estimated using Kaplan–Meier curves. Log-rank test and adjusted Cox proportional hazard modelling were used to test for differences in survival distributions and determine AKI effect on survival function, respectively. AKI occurred in 70 (19.3%), with 21.4% in-hospital mortality for AKI group. Younger age, lower weight, longer cardiopulmonary bypass time, preoperative mechanical ventilation and diagnostic category were associated with postoperative AKI. Resolution rate was 92.7% prior to hospital discharge for survivors. AKI was associated with longer duration of mechanical ventilation, ICU and hospital length of stay. AKI patients had significantly higher probability of all-cause mortality postoperatively when compared to the non-AKI group (log-rank test, p < 0.001). Adjusted hazard ratio for AKI versus non-AKI group was 11.08 (95% CI 2.45–50.01; p = 0.002). Diagnostic category was associated with cardiac surgery-related AKI in children with CHD, a finding supporting the development of lesion specific models for risk stratification. Postoperative AKI had detrimental impact on clinical outcomes and was associated with decreased survival to hospital discharge.
Intra-Individual Changes in Total Procollagen-Type 1 N-terminal Propeptide in a Korean Adult Population
Authors: Choi Rihwa , Lee Sang Gon , Lee Eun Hee
Published: October 2022

We aimed to investigate intra-individual changes in total procollagen-type 1 N-terminal pro-peptide (P1NP), a biochemical marker of bone turnover, to understand patient populations and test utilization in a Korean adult population while considering different definitions of least significant changes by sex, age, and medical institution type. Overall, 31,501 P1NP tests were performed on 24,644 Korean adults (3389 men and 21,255 women) with a median age of 68.9 years (interquartile range, IQR, 61.2–77.2) for osteoporosis evaluation. Among these, 1331 (5.4%) patients (127 men and 1204 women) underwent ≥3 follow-up P1NP measurements. The median follow-up period was 12.5 months (IQR, 11.7–15.9). Among 1331 patients, 64.4% experienced a decrease in P1NP and 35.6% experienced an increase in P1NP during follow-up. Among these, the proportion of patients who experienced serum P1NP changes ≥14.4% from baseline was 92.3%, and the proportion of patients who achieved ≤40 ng/mL (a median level of premenopausal Korean women) during follow-up was 31.8%. The overall proportion of patients that experienced a serum P1NP change exceeding the least significant change during follow-up was not significantly different by the type of medical institution.
Inter/Intra-Observer Agreement in Video-Capsule Endoscopy: Are We Getting It All Wrong? A Systematic Review and Meta-Analysis
Authors: Cortegoso Valdivia Pablo , Deding Ulrik , Bjørsum-Meyer Thomas , Baatrup Gunnar , Fernández-Urién Ignacio , Dray Xavier , Boal-Carvalho Pedro , Ellul Pierre , Toth Ervin , Rondonotti Emanuele , Kaalby Lasse , Pennazio Marco , Koulaouzidis Anastasios
Published: October 2022

Video-capsule endoscopy (VCE) reading is a time- and energy-consuming task. Agreement on findings between readers (either different or the same) is a crucial point for increasing performance and providing valid reports. The aim of this systematic review with meta-analysis is to provide an evaluation of inter/intra-observer agreement in VCE reading. A systematic literature search in PubMed, Embase and Web of Science was performed throughout September 2022. The degree of observer agreement, expressed with different test statistics, was extracted. As different statistics are not directly comparable, our analyses were stratified by type of test statistics, dividing them in groups of “None/Poor/Minimal”, “Moderate/Weak/Fair”, “Good/Excellent/Strong” and “Perfect/Almost perfect” to report the proportions of each. In total, 60 studies were included in the analysis, with a total of 579 comparisons. The quality of included studies, assessed with the MINORS score, was sufficient in 52/60 studies. The most common test statistics were the Kappa statistics for categorical outcomes (424 comparisons) and the intra-class correlation coefficient (ICC) for continuous outcomes (73 comparisons). In the overall comparison of inter-observer agreement, only 23% were evaluated as “good” or “perfect”; for intra-observer agreement, this was the case in 36%. Sources of heterogeneity (high, I2 81.8–98.1%) were investigated with meta-regressions, showing a possible role of country, capsule type and year of publication in Kappa inter-observer agreement. VCE reading suffers from substantial heterogeneity and sub-optimal agreement in both inter- and intra-observer evaluation. Artificial-intelligence-based tools and the adoption of a unified terminology may progressively enhance levels of agreement in VCE reading.
Nuclear Expression of β-Catenin Is Associated with Improved Outcomes in Endometrial Cancer
Authors: Masciullo Valeria , Susini Tommaso , Corrado Giacomo , Stepanova Marina , Baroni Alessandro , Renda Irene , Castiglione Francesca , Minimo Corrado , Bellacosa Alfonso , Chiofalo Benito , Vizza Enrico , Scambia Giovanni
Published: October 2022

Beta-catenin is involved in intercellular adhesion and participates in the Wnt signaling pathway. This study evaluated the expression pattern and prognostic value of β-catenin in a series of endometrial carcinoma patients. Immunohistochemical analyses were used to assess the expression and subcellular localization of β-catenin from tissue sections of 74 patients with endometrial carcinoma. No correlation was found between beta-catenin expression and clinicopathological parameters. Patients expressing nuclear β-catenin (n = 13; 16%) showed a more favorable prognosis than patients expressing membranous β-catenin; the 5-year disease-related survival rate was 100% for cases expressing nuclear β-catenin, compared with 73.8% (SE 0.08) of cases expressing membranous β-catenin (p = 0.04). Although statistical significance was not reached (p = 0.15), cases expressing nuclear β-catenin showed a 5-year disease-free survival rate of 90.9% (SE 0.08) compared with 67.4% (SE 0.08) of cases expressing membranous β-catenin. Univariate Cox analysis revealed that membranous β-catenin expression was found to be associated with a relative risk of death of 33.9 (p = 0.04). The stage of disease (p = 0.0006), histology (p = 0.003), and grading (p = 0.008) were also significantly correlated with disease-free survival according to univariate Cox analyses. Determining β-catenin expression and localization patterns may predict survival in patients with endometrial cancer and, therefore, should be considered a potential prognostic marker of disease.