bluuebunny/crossref_metadata_2025 huggingface dataset:
The Association between Mycoplasma pneumoniae Genotype and Cutaneous Disease
Authors: Rodman Berlot Jasna , Mrvič Tatjana , Košnik Mitja , Keše Darja
Published: January 2023

Mycoplasma pneumoniae (Mp) can cause several extrapulmonary manifestations, most frequently dermatological ones. It is largely unknown whether Mp genotype determines Mp-induced cutaneous disease. The aim of our study was to assess the association between Mp genotype and this clinical outcome. We performed a retrospective study of children referred with signs of acute Mp infection from 1 January 2014 to 31 December 2014. We compared the characteristics of children presenting as cutaneous disease, upper (URTI) and lower respiratory tract infection (LRTI). In addition, we separately analyzed the data of patients presenting with Mp-induced cutaneous disease. We evaluated data from 435 patients (mean age 7.3 years, SD 3.4 years; 52.0% boys) who had Mp PCR-positive pharyngeal swab, P1 genotype and/or multilocus variable-number tandem-repeat analysis (MLVA) genotype defined and no viral co-detection, presenting as cutaneous disease (38/435), URTI (46/435) or LRTI (351/435). The majority of patients had urticarial (55%, 21/38) or maculopapular eruptions (37%, 14/38). We found no association between Mp genotype and clinical outcome of cutaneous disease, nor any specific dermatological presentation. In the group with cutaneous disease, 18% (7/38) required hospital admission because of rash. We found that infection with MLVA-3,6,6,2 strains was more common in admitted patients than in outpatients (40% vs. 4%, p = 0.017) and significantly affected the likelihood of hospital admission in a logistic regression model. The results of our cohort study suggest that Mp genotype does not determine Mp-induced cutaneous disease or a specific dermatological presentation. Nevertheless, infections with certain MLVA strains could induce more severe cutaneous disease requiring hospitalization.
Calendula officinalis—A Great Source of Plant Growth Promoting Endophytic Bacteria (PGPEB) and Biological Control Agents (BCA)
Authors: Tsalgatidou Polina C. , Thomloudi Eirini-Evangelia , Nifakos Kallimachos , Delis Costas , Venieraki Anastasia , Katinakis Panagiotis
Published: January 2023

The application of beneficial bacteria may present an alternative approach to chemical plant protection and fertilization products as they enhance growth and resistance to biotic and abiotic stresses. Plant growth-promoting bacteria are found in the rhizosphere, epiphytically or endophytically (Plant Growth Promoting Endophytic Bacteria, PGPEB). In the present study, 36 out of 119 isolated endophytic bacterial strains from roots, leaves and flowers of the pharmaceutical plant Calendula officinalis were further identified and classified into Bacillus, Pseudomonas, Pantoea, Stenotrophomonas and Rhizobium genera. Selected endophytes were evaluated depending on positive reaction to different plant growth promoting (PGP) traits, motility, survival rate and inhibition of phytopathogenic fungi in vitro and ex vivo (tomato fruit). Bacteria were further assessed for their plant growth effect on Arabidopsis thaliana seedlings and on seed bio-primed tomato plantlets, in vitro. Our results indicated that many bacterial endophytes increased seed germination, promoted plant growth and changed root structure by increasing lateral root density and length and root hair formation. The most promising antagonistic PGPEB strains (Cal.r.29, Cal.l.30, Cal.f.4, Cal.l.11, Cal.f.2.1, Cal.r.19 and Cal.r.11) are indicated as effective biological control agents (BCA) against Botrytis cinerea on detached tomato fruits. Results underlie the utility of beneficial endophytic bacteria for sustainable and efficient crop production and disease control.
Research on Roof Load Transfer by Passing Coal Pillar of Working Face in Shallow Buried Closely Multiple-Seam
Authors: He Yanpeng , Huang Qingxiang , Wei Yehao , Du Junwu
Published: January 2023

The dynamic load effect of supports is mainly caused by the movement of the roof structure and the load transfer of overburden. In view of the practice issue that the phenomenon of strong ground pressure is easy to happen, when the working face of the lower coal seam passes the inclined coal pillar in shallow buried closely multiple-seam, it will lead to supprot damaged. This paper takes the mining of over-inclined coal pillars in the 22410 working face of the Bulianta Coal Mine as the background, based on the research method combining the field measurement, physical simulation experiment, and numerical calculation, the evolution law of the front abutment pressure (FAP) and roof weighting in mining under the inclined coal pillar is analyzed, and the mechanism of the stress transfer of the inclined coal pillar and the dynamic load of the support is revealed. The research shows that the concentrated stress of the coal pillar is jointly borne by the front coal wall of the working face and the interburden structure above the support. The vertical stress transmitted from the coal pillar to the floor acts on the key blocks of the interburden of the lower coal seam, which causes strong pressure and dynamic load effect, such as roof structure cut-off. The periodic breaking of the key stratum of the interburden leads to the development height and range of the cracks increasing stepwise. The partition characteristics of the mutual transformation of the interburden stress, the FAP, and the working resistance (WR) by passing the coal pillar stage are revealed, which is divided into three stages and four regions. With the working face passing through the inclined coal pillar, the influence area of the concentrated stress of the coal pillar is reduced, and the peak stress of the coal pillar is gradually transferred to the outside of the coal pillar. When the working face is 5 m away from the coal pillar, the peak of FAP and WR reaches the maximum values, the roof is cutting along the peak stress line, and the working face has a strong weighting phenomenon. The research results are consistent with the field measurement results, providing a reference for the mining of working faces under similar conditions.
An Attention towards the Prophylactic and Therapeutic Options of Phytochemicals for SARS-CoV-2: A Molecular Insight
Authors: Shoaib Shoaib , Ansari Mohammad Azam , Kandasamy Geetha , Vasudevan Rajalakshimi , Hani Umme , Chauhan Waseem , Alhumaidi Maryam S. , Altammar Khadijah A. , Azmi Sarfuddin , Ahmad Wasim , Wahab Shadma , Islam Najmul
Published: January 2023

The novel pathogenic virus was discovered in Wuhan, China (December 2019), and quickly spread throughout the world. Further analysis revealed that the pathogenic strain of virus was corona but it was distinct from other coronavirus strains, and thus it was renamed 2019-nCoV or SARS-CoV-2. This coronavirus shares many characteristics with other coronaviruses, including SARS-CoV and MERS-CoV. The clinical manifestations raised in the form of a cytokine storm trigger a complicated spectrum of pathophysiological changes that include cardiovascular, kidney, and liver problems. The lack of an effective treatment strategy has imposed a health and socio-economic burden. Even though the mortality rate of patients with this disease is lower, since it is judged to be the most contagious, it is considered more lethal. Globally, the researchers are continuously engaged to develop and identify possible preventive and therapeutic regimens for the management of disease. Notably, to combat SARS-CoV-2, various vaccine types have been developed and are currently being tested in clinical trials; these have also been used as a health emergency during a pandemic. Despite this, many old antiviral and other drugs (such as chloroquine/hydroxychloroquine, corticosteroids, and so on) are still used in various countries as emergency medicine. Plant-based products have been reported to be safe as alternative options for several infectious and non-infectious diseases, as many of them showed chemopreventive and chemotherapeutic effects in the case of tuberculosis, cancer, malaria, diabetes, cardiac problems, and others. Therefore, plant-derived products may play crucial roles in improving health for a variety of ailments by providing a variety of effective cures. Due to current therapeutic repurposing efforts against this newly discovered virus, we attempted to outline many plant-based compounds in this review to aid in the fight against SARS-CoV-2.
Does Beetroot Supplementation Improve Performance in Combat Sports Athletes? A Systematic Review of Randomized Controlled Trials
Authors: Delleli Slaheddine , Ouergui Ibrahim , Messaoudi Hamdi , Trabelsi Khaled , Glenn Jordan M. , Ammar Achraf , Chtourou Hamdi
Published: January 2023

While studies on dietary nitrate (NO3−) supplementation and its impact on combat sports performance are increasing, finite conclusions from currently available investigations remain unclear. Thus, the present systematic review examined the acute and chronic ergogenic effect(s) of dietary nitrate intake from beetroot on different aspects of combat sports performance. A systematic search for randomized placebo-controlled studies investigating the effects of beetroot supplementation on combat sports outcomes was performed through Scopus, PubMed/MEDLINE, Web of Science, Scielo, Sport Discus, and Cochrane Library databases up to 2 January 2023. The different terms related to beetroot and to combat sports were connected in the search strategies using the Boolean operators ‘AND’ and ‘OR’. A total of nine studies with good methodological quality (based on the Cochrane risk of bias tool) fulfilled the inclusion criteria. Seven studies used an acute supplementation strategy, while the other two studies utilized chronic supplementation. Findings showed beetroot intake may be an effective tool to improve oxidative metabolism and muscle force production (i.e., isokinetic and isometric) in combat sports athletes. However, these effects may depend on the population, intake duration, muscle group activated, and exercise type. Future studies are required to (1) understand the effects on female athletes and (2) elucidate the impacts of dosing protocols and specific exercise modalities for enhancing combat sports performance.
Age Influences on Lifestyle and Stress Perception in the Working Population
Authors: Lucini Daniela , Pagani Eleonora , Capria Francesco , Galiano Michele , Marchese Marcello , Cribellati Stefano , Parati Gianfranco
Published: January 2023

Workplace health promotion programs and services offered by insurers may play a fundamental role to foster health/well-being and to prevent chronic diseases. To this end, they should be tailored to companies/employees’ requirements and characteristics. In particular, age needs to be taken into account, considering both that young age workers are generally healthy, and that young age is the best period in lifespan to address prevention and instilling healthy behaviors. We employed an anonymous, simple web-based questionnaire (filled out by 1305 employees) which furnishes data regarding lifestyle (nutrition, exercise, smoking, stress, sleep, etc.), some of which were used to build a unique descriptor (Lifestyle Index; 0–100 higher scores being healthier). We considered three subgroups accordingly to age: ≤30; between 30 and 50; >50 years. This study showed age influences lifestyle and stress perception in the working population: the youngest employees (both men and women) presented the worst lifestyle index, particularly in its stress component. This observation may potentially be useful to tailor workplace health promotion programs and to personalize insurance protocols and services offered to employees. The practical message of our study is that in healthy young people focusing only on medical parameters (frequently within normal ranges in this cohort), albeit important, may be not sufficient to foster proactive actions to prevent chronic non-communicable diseases in adult life. Vice versa, driving their attention on current behaviors might elicit their proactive role to improve lifestyle, getting immediate advantages such as well-being improvement and the possibility to best manage stress.
Interrelationships between Dietary Outcomes, Readmission Rates and Length of Stay in Hospitalised Oncology Patients: A Scoping Review
Authors: MacFarling Meure Cecelia , Steer Belinda , Porter Judi
Published: January 2023

Background: Poor food intake is an independent risk factor for malnutrition in oncology patients, and achieving adequate nutrition is essential for optimal clinical and health outcomes. This review investigated the interrelationships between dietary intakes, hospital readmissions and length of stay in hospitalised adult oncology patients. Methodology: Three databases, MEDLINE, Web of Science and PubMed were searched for relevant publications from January 2000 to the end of August 2022. Results: Eleven studies investigating the effects of dietary intakes on length of stay (LOS) and hospital readmissions in cohorts of hospitalised patients that included oncology patients were identified. Heterogenous study design, nutritional interventions and study populations limited comparisons; however, a meta-analysis of two randomised controlled trials comparing dietary interventions in mixed patient cohorts including oncology patients showed no effect on LOS: mean difference −0.08 (95% confidence interval −0.64–0.49) days (p = 0.79). Conclusions: Despite research showing the benefits of nutritional intake during hospitalisation, evidence is emerging that the relationship between intakes, LOS and hospital readmissions may be confounded by nutritional status and cancer diagnosis.
The Antioxidant Potential of Graviola and Its Potential Medicinal Application
Authors: Olas Beata
Published: January 2023

Graviola (Annunona muricata L.), a plant growing in tropical regions, has many names and a range of ethnomedicinal uses. The leaves are used to treat insomnia, diabetes, cystitis, and headaches, the crushed seeds have anthelmintic properties, and the fruits are used in the preparation of ice creams, candy, syrups, shakes, and other beverages. The key active components are believed to be annonaceous acetogenins, with more than 100 such compounds having been isolated from A. muricata. The plant is also a source of a range of phenolic compounds, essential oils, alkaloids, flavonol triglycosides, and megastigmanes, together with various minerals, including Mg, Fe, Cu, K, and Ca. Its key phenolic compounds are rutin, kaempferol, and quercetin. This paper provides an overview of the current state of knowledge about the antioxidant properties of various graviola organs and their major constituents, based on a review of various electronic databases. However, few findings have been obtained from clinical trials, and few in vitro and animal studies suggest that graviola preparations have antioxidant properties; as such, the antioxidant potential of graviola, and its safety, remain unclear.
SLC6A4 DNA Methylation Levels and Serum Kynurenine/Tryptophan Ratio in Eating Disorders: A Possible Link with Psychopathological Traits?
Authors: Franzago Marica , Orecchini Elena , Porreca Annamaria , Mondanelli Giada , Orabona Ciriana , Dalla Ragione Laura , Di Nicola Marta , Stuppia Liborio , Vitacolonna Ester , Beccari Tommaso , Ceccarini Maria Rachele
Published: January 2023

Background: The incidence of eating disorders (EDs), serious mental and physical conditions characterized by a disturbance in eating or eating-related behaviors, has increased steadily. The present study aims to develop insights into the pathophysiology of EDs, spanning over biochemical, epigenetic, psychopathological, and clinical data. In particular, we focused our attention on the relationship between (i) DNA methylation profiles at promoter-associated CpG sites of the SCL6A4 gene, (ii) serum kynurenine/tryptophan levels and ratio (Kyn/Trp), and (iii) psychopathological traits in a cohort of ED patients. Among these, 45 patients were affected by restricting anorexia nervosa (AN0), 21 by purging AN (AN1), 21 by bulimia (BN), 31 by binge eating disorders (BED), 23 by unspecified feeding or eating disorders (UFED), and finally 14 by other specified eating disorders (OSFED) were compared to 34 healthy controls (CTRs). Results: Kyn level was higher in BED, UFED, and OSFED compared to CTRs (p ≤ 0.001). On the other hand, AN0, AN1, and BN patients showed significatively lower Kyn levels compared to the other three ED groups but were closed to CTRs. Trp was significantly higher in AN0, AN1, and BN in comparison to other ED groups. Moreover, AN1 and BN showed more relevant Trp levels than CTRs (p <0.001). BED patients showed a lower Trp as compared with CTRs (p ≤ 0.001). In addition, Kyn/Trp ratio was lower in the AN1 subtype but higher in BED, UFED, and OSFED patients than in CTRs (p ≤ 0.001). SCL6A4 DNA methylation level at CpG5 was lower in AN0 compared to BED (p = 0.021), and the CpG6 methylation was also significantly lower in AN0 in comparison to CTRs (p = 0.025). The mean methylation levels of the six CpGs analyzed were lower only in the AN0 subgroup compared to CTRs (p = 0.008). Relevant psychological trait EDI-3 subscales were correlated with biochemical and epigenetic data. Conclusions: These findings underline the complexity of psychological and pathophysiological components of EDs.
Development and Implementation of a Nutritional Education Program Aimed at Improving the Integration Process of Young Orphan Refugees Newly Arrived in Portugal
Authors: Oliveira Bárbara , Bicho Manuel , Valente Ana
Published: January 2023

(1) Background: Refugees are a population group at imminent risk of death, being forced to migrate to countries with different cultures. Many of the refugees are at great risk of malnutrition, especially adolescent orphans. The aim of the study was to establish a nutritional and food education program to improve the integration process of young orphan refugees newly arrived in Portugal. (2) Methods: A nutrition and food education program with nine sessions of food and nutrition education over 12 weeks was carried out by a nutritionist from March to June 2016, in 15 young residents of the Reception Center for Refugee Children. The program included the application of a nutritional knowledge questionnaire, an anthropometric assessment, and the collection of data on food habits and lifestyle. The evaluation of the program was carried out by comparing the initial and final scores of the nutritional knowledge questionnaire. (3) Results: There was an improvement in nutritional knowledge among the adolescents, and a direct relationship between attendance at the sessions and improvement of this knowledge was found. Non-significant changes were observed in some anthropometric measurements between the beginning and the ending of the program. (4) Conclusions: This food education program contributed to a better understanding by young orphan refugees newly arrived in Portugal of the foods available in Portugal and of the Portuguese gastronomy.
Consumers’ Perceptions of the Design of Front-of-Package Warning Labels—A Qualitative Study in China
Authors: Zhang Xiaofan , Ouyang Yifei , Yin Xuejun , Bai Jing , Zhang Run , Zhang Jiguo , Wang Qi , Wang Huijun
Published: January 2023

The warning label is identified as a simple front-of-package labeling format that assists consumers in making healthier food choices at the point of purchase. The color and/or shape of the design elements of the packaging labels can influence the understanding and purchase intention outcomes. This study aimed to explore the perceptions of consumers regarding differently designed warning labels (WLs) to identify a possibly suitable warning label design in the Chinese context. Using a qualitative approach, we collected data through 12 focus group discussions consisting of 116 participants residing in 6 provinces. Participants were selected by purposive sampling. Data were coded following inductive thematic analysis and summarized into three themes: (1) perceived preference for the appearance of the WLs, (2) perceived benefits of the WLs, and (3) suggestions for promoting the WLs. Participants preferred the black shield label showing a high content of the nutrients of concern (e.g., sodium, added sugar, and saturated fat) in both Chinese and English. Consumers generally agreed that the WLs were easy to understand and offered a simple method for choosing healthier foods. They anticipated that WLs could encourage the food industry to reformulate their products. Our study results will support the design and facilitate the use of WLs in China.
Interlinkages between Climate Change and Food Systems: The Impact on Child Malnutrition—Narrative Review
Authors: Agostoni Carlo , Baglioni Mattia , La Vecchia Adriano , Molari Giulia , Berti Cristiana
Published: January 2023

The pandemics of obesity, undernutrition, and climate change represent severe threats to child health. They co-occur; interact with each other to produce sequelae at biological, psychological, or social levels; and share common underlying drivers. In this paper, we review the key issues concerning child diet and nutritional status, focusing on the interactions with climate and food systems. Inadequate infant and young child feeding practices, food insecurity, poverty, and limited access to health services are the leading causes of malnutrition across generations. Food system industrialization and globalization lead to a double burden of malnutrition, whereby undernutrition (i.e., stunting, wasting, and deficiencies in micronutrients) coexists with overweight and obesity, as well as to harmful effects on climate. Climate change and the COVID-19 pandemic are worsening child malnutrition, impacting the main underlying causes (i.e., household food security, dietary diversity, nutrient quality, and access to maternal and child health), as well as the social, economic, and political factors determining food security and nutrition (livelihoods, income, infrastructure resources, and political context). Existing interventions have the potential to be further scaled-up to concurrently address undernutrition, overnutrition, and climate change by cross-cutting education, agriculture, food systems, and social safety nets. Several stakeholders must work co-operatively to improve global sustainable nutrition.
Underlying Mechanisms Involved in Gambling Disorder Severity: A Pathway Analysis Considering Genetic, Psychosocial, and Clinical Variables
Authors: Solé-Morata Neus , Baenas Isabel , Etxandi Mikel , Granero Roser , Gené Manel , Barrot Carme , Gómez-Peña Mónica , Moragas Laura , Ramoz Nicolas , Gorwood Philip , Fernández-Aranda Fernando , Jiménez-Murcia Susana
Published: January 2023

Gambling Disorder (GD) has a complex etiology that involves biological and environmental aspects. From a genetic perspective, neurotrophic factors (NTFs) polymorphisms have been associated with the risk of developing GD. The aim of this study was to assess the underlying mechanisms implicated in GD severity by considering the direct and mediational relationship between different variables including genetic, psychological, socio-demographic, and clinical factors. To do so, we used genetic variants that were significantly associated with an increased risk for GD and evaluated its relationship with GD severity through pathway analysis. We found that the interaction between these genetic variants and other different biopsychological features predicted a higher severity of GD. On the one hand, the presence of haplotype block 2, interrelated with haplotype block 3, was linked to a more dysfunctional personality profile and a worse psychopathological state, which, in turn, had a direct link with GD severity. On the other hand, having rs3763614 predicted higher general psychopathology and therefore, higher GD severity. The current study described the presence of complex interactions between biopsychosocial variables previously associated with the etiopathogenesis and severity of GD, while also supporting the involvement of genetic variants from the NTF family.
Metformin Induces Apoptosis in Human Pancreatic Cancer (PC) Cells Accompanied by Changes in the Levels of Histone Acetyltransferases (Particularly, p300/CBP-Associated Factor (PCAF) Protein Levels)
Authors: Szymczak-Pajor Izabela , Drzewoski Józef , Świderska Ewa , Strycharz Justyna , Gabryanczyk Anna , Kasznicki Jacek , Bogdańska Marta , Śliwińska Agnieszka
Published: January 2023

Accumulating evidence (mainly from experimental research) suggests that metformin possesses anticancer properties through the induction of apoptosis and inhibition of the growth and proliferation of cancer cells. However, its effect on the enzymes responsible for histone acetylation status, which plays a key role in carcinogenesis, remains unclear. Therefore, the aim of our study was to evaluate the impact of metformin on histone acetyltransferases (HATs) (i.e., p300/CBP-associated factor (PCAF), p300, and CBP) and on histone deacetylases (HDACs) (i.e., SIRT-1 in human pancreatic cancer (PC) cell lines, 1.2B4, and PANC-1). The cells were exposed to metformin, an HAT inhibitor (HATi), or a combination of an HATi with metformin for 24, 48, or 72 h. Cell viability was determined using an MTT assay, and the percentage of early apoptotic cells was determined with an Annexin V-Cy3 Apoptosis Detection Assay Kit. Caspase-9 activity was also assessed. SIRT-1, PCAF, p300, and CBP expression were determined at the mRNA and protein levels using RT-PCR and Western blotting methods, respectively. Our results reveal an increase in caspase-9 in response to the metformin, indicating that it induced the apoptotic death of both 1.2B4 and PANC-1 cells. The number of cells in early apoptosis and the activity of caspase-9 decreased when treated with an HATi alone or a combination of an HATi with metformin, as compared to metformin alone. Moreover, metformin, an HATi, and a combination of an HATi with metformin also modified the mRNA expression of SIRT-1, PCAF, CBP, and p300. However, metformin did not change the expression of the studied genes in 1.2B4 cells. The results of the Western blot analysis showed that metformin diminished the protein expression of PCAF in both the 1.2B4 and PANC-1 cells. Hence, it appears possible that PCAF may be involved in the metformin-mediated apoptosis of PC cells.
The Effects of Different Doses of Sildenafil on Coronary Blood Flow and Oxidative Stress in Isolated Rat Hearts
Authors: Banjac Nada , Vasović Velibor , Stilinović Nebojša , Tomas Ana , Vasović Lucija , Martić Nikola , Prodanović Dušan , Jakovljević Vladimir
Published: January 2023

The dose-response relationship of sildenafil effects on cardiac function is not completely elucidated. The aim of this study was to assess the effects of different doses of sildenafil on coronary flow and oxidative stress in isolated rat hearts. Coronary flow and markers of oxidative stress, including nitrite outflow, and superoxide anion production in coronary effluent, were determined for isolated rat hearts. The experiments were performed during control conditions and in the presence of sildenafil (10, 20, 50, 200 nM) alone or with Nω-nitro-L-arginine monomethyl ester (L-NAME) (30 μM). Sildenafil was shown to result in a significant increase in coronary flow at lower coronary perfusion pressure (CPP) values at all administered doses, whereas, with an increase in CPP, a reduction in coronary flow was observed. An increase in nitric oxide (NO) was most pronounced in the group treated with the lowest dose of sildenafil at the highest CPP value. After the inhibition of the NO-cyclic guanosine monophosphate (cGMP) signaling (NOS) system by L-NAME, only a dose of 200 nM sildenafil was high enough to overcome the inhibition and to boost release of O2−. That effect was CPP-dependent, with statistical significance reached at 80, 100 and 120 mmHg. Our findings indicate that sildenafil causes changes in heart vasculature in a dose-dependent manner, with a shift from a vasodilatation effect to vasoconstriction with a pressure increase. The highest dose administered is capable of producing superoxide anion radicals in terms of NOS system inhibition.
In Vitro–In Vivo Correlations (IVIVC) for Predicting the Clinical Performance of Metronidazole Topical Creams Intended for Local Action
Authors: Rath Seeprarani , Kanfer Isadore
Published: January 2023

The safety and efficacy of a generic medicine can be confirmed by demonstrating bioequivalence (BE) between the generic product and its reference listed drug (RLD) by measuring drug concentrations in the blood following administration. However, for topical dermatological products that are not absorbed into the systemic circulation, clinical trials in patients are required. The objective of this investigation was to use an in vitro method to predict in vivo performance by correlating in vitro release testing (IVRT) data with tape stripping (TS) data following the application of metronidazole (MTZ) creams to the skin of healthy human participants. Whereas IVRT is generally used to characterize the release of a drug from topical products across a synthetic membrane into a suitable receptor medium, TS involves the sequential removal of layers of stratum corneum (SC) with an adhesive tape to determine the amount of the drug in the skin. The resulting IVRT and TS data were correlated using the IVRT parameter of the apparent release constant (ARC), which is the slope obtained from the release rate profile, with the TS parameter of the area under the curve (AUC) obtained from a plot of the amount of drug per tape strip vs. the relative SC depth. A rank order relationship for these parameters was established for the reference and test products. A graph of AUC vs. ARC was plotted to establish a Level C in vitro–in vivo correlation (IVIVC). Although the ARC for T1 was slightly lower than that for the reference, the rank order was essentially consistent. A linear relationship was observed between the AUCs and ARCs. The equation derived was used to predict the AUCs for all the tested products based on their respective ARCs. The predicted AUC values based on the observed ARCs were similar to the observed AUCs. The lower and upper limits for the in vitro and in vivo parameters for BE were computed based on regulatory acceptance criteria. In order to predict BE from the IVRT studies, the values of the ARC should be between 30.50 and 47.67 when comparing test and reference cream products containing MTZ.
Design of A3B-Porphyrin Conjugates with Terpyridine as Potential Theranostic Agents: Synthesis, Complexation with Fe(III), Gd(III), and Photodynamic Activity
Authors: Zhdanova Kseniya A. , Ivantsova Anastasia V. , Vyalba Fedor Yu. , Usachev Maxim N. , Gradova Margarita A. , Gradov Oleg V. , Karpechenko Natalia Yu. , Bragina Natal’ya A.
Published: January 2023

This paper reports on the design and synthesis of new multifunctional porphyrin-based therapeutic agents for potential therapeutic and diagnostic applications. Zinc complexes of A3B-type meso-arylporphyrins containing OH- and COOH- groups were modified with chelating ligands based on 4′-(4-methylphenyl)-2,2′:6′,2″-terpyridine derivatives in high yields. Novel complexes with Gd(III), Fe(III) were obtained for these conjugates. Aggregation behaviour in solutions of different solubilisers was studied to inform the selection of the optimal solubilising platform for the porphyrins obtained; their photophysical and photochemical properties were also characterised. Micellar Pluronic F127 formulation was found to be the most effective solubiliser for stabilising the fluorescence-active monomolecular form of the photosensitisers (PS). In vitro cytotoxicity of the compounds was studied on the HEP-2 cell line with and without irradiation for 1.5 and 24 h. As a result, the IC50 of compounds 12 and 14 at an irradiation dose of 8.073 J/cm2 was shown to be 1.87 ± 0.333 and 1.4 ± 0.152 μM, respectively; without irradiation, the compound had no toxic effect within the studied concentration range (1.5 h). A test for the inhibition of metabolic cooperation or promoter activity was also performed for the abovementioned compounds, showing the efficacy and safety of the conjugates obtained. Preliminary data have indicated the high potential of the new type of PS to be promising molecular theranostic agents.
Natural Compounds and Biopolymers-Based Hydrogels Join Forces to Promote Wound Healing
Authors: Falbo Federica , Spizzirri Umile Gianfranco , Restuccia Donatella , Aiello Francesca
Published: January 2023

Rapid and complete wound healing is a clinical emergency, mainly in pathological conditions such as Type 2 Diabetes mellitus. Many therapeutic tools are not resolutive, and the research for a more efficient remedial remains a challenge. Wound dressings play an essential role in diabetic wound healing. In particular, biocompatible hydrogels represent the most attractive wound dressings due to their ability to retain moisture as well as ability to act as a barrier against bacteria. In the last years, different functionalized hydrogels have been proposed as wound dressing materials, showing encouraging outcomes with great benefits in the healing of the diabetic wounds. Specifically, because of their excellent biocompatibility and biodegradability, natural bioactive compounds, as well as biomacromolecules such as polysaccharides and protein, are usually employed in the biomedical field. In this review, readers can find the main discoveries regarding the employment of naturally occurring compounds and biopolymers as wound healing promoters with antibacterial activity. The emerging approaches and engineered devices for effective wound care in diabetic patients are reported and deeply investigated.
Chemopreventive and Anticancer Role of Resveratrol against Oral Squamous Cell Carcinoma
Authors: Angellotti Giuseppe , Di Prima Giulia , Belfiore Elena , Campisi Giuseppina , De Caro Viviana
Published: January 2023

Oral squamous cell carcinoma (OSCC) is one of the most prevailing and aggressive head and neck cancers, featuring high morbidity and mortality. The available conventional treatments suffer from several adverse effects and are often inefficient in terms of their survival rates. Thus, seeking novel therapeutic agents and adjuvants is of the utmost importance for modern society. Natural polyphenolic compounds have recently emerged as promising chemopreventive and anticancer agents. Specifically, the natural compound resveratrol (RSV) has recently gained momentum for this purpose. RSV is useful for treating OSCC due to its antiproliferative, antimetastatic, and proapoptotic effects. Additionally, RSV acts against tumor cells while synergically cooperating with chemotherapeutics, overcoming drug resistance phenomena. Despite these wide-spectrum effects, there are few specific investigations regarding RSV’s effects against OSCC animal models that consider different routes and vehicles for the administration of RSV. Interestingly, an injectable RSV-loaded liposome-based formulation was proven to be effective against both in vitro and in vivo OSCC models, demonstrating that the development of RSV-loaded drug delivery systems for systemic and/or loco-regional applications may be the turning point in oral cancer treatment, leading to benefits from both RSV’s properties as well as from targeted delivery. Given these premises, this review offers a comprehensive overview of the in vitro and in vivo effects of RSV and its main derivative, polydatin (PD), against OSCC-related cell lines and animal models, aiming to guide the scientific community in regard to RSV and PD use in the treatment of oral precancerous and cancerous lesions.
Correction: Zare et al. Encapsulation of miRNA and siRNA into Nanomaterials for Cancer Therapeutics. Pharmaceutics 2022, 14, 1620
Authors: Zare Mina , Pemmada Rakesh , Madhavan Maya , Shailaja Aswathy , Ramakrishna Seeram , Kandiyil Sumodan Padikkala , Donahue James M. , Thomas Vinoy
Published: January 2023

In the original publication [...]
Special Issue on Optical Quantum Manipulation of Rydberg Atoms
Authors: Yan Dong , Wu Jin-Hui
Published: January 2023

Rydberg atoms with large electric dipole moments, strong dipole–dipole interactions, and long radiative lifetimes have attracted great attention and become the subject of intense studies in the past two decades [...]
Unraveling Large and Polyploidy Genome of the Crucifer Orychophragmus violaceus in China, a Potential Oil Crop
Authors: Pan Qi , Zeng Pan , Li Zaiyun
Published: January 2023

The genus Orychophragmus in the Brassicaceae family includes the types with 2n = 20, 22, 24, and 48. The species O. violaceus (L.) O. E. Schulz has 2n = 24 and is widely cultivated as an ornamental plant in China. This review summarizes the research progress of its genome structure and evolution in the context of cytogenetics and genome sequencing. This species has a large genome size of ~1 Gb and longer chromosomes than those of Brassica species, which is attributable to the burst of TE insertions. Even more, one tetraploidization event from about 600–800 million years ago is elucidated to occur during its genome evolution, which is consistent with the polyploidy nature of its genome revealed by the meiotic pairing patterns. Its chromosomes are still characterized by a larger size and deeper staining than those from Brassica species in their intergeneric hybrids, which is likely related to their inherent differences between genome structures and cytology. Its genome is dissected by the development of additional alien lines, and some traits are located on individual chromosomes. Due to the abundant dihydroxy fatty acids in its seed oil with superior lubricant properties and wide environmental adaptations, this plant promises to be utilized as one new oil crop in the future.
One-Pot Tandem Alcoholysis-Hydrogenation of Polylactic Acid to 1,2-Propanediol
Authors: Xu Jialin , Zhou Kuo , Qin Linlin , Tan Zaiming , Huang Shijing , Duan Peigao , Kang Shimin
Published: January 2023

The chemical recycling of end-of-life polylactic acid (PLA) plays roles in mitigating environmental pressure and developing circular economy. In this regard, one-pot tandem alcoholysis and hydrogenation of PLA was carried out to produce 1,2-propanediol, which is a bulk chemical in polymer chemistry. In more detail, the commercially available Raney Co was employed as the catalyst, and transformation was conducted in ethanol, which acted as nucleophilic reagent and solvent. Single-factor analysis and Box–Behnken design were used to optimize the reaction conditions. Under the optimized condition, three kinds of PLA materials were subjected to degradation. Additionally, 74.8 ± 5.5%, 76.5 ± 6.2%, and 71.4 ± 5.7% of 1,2-propanediol was yielded from PLA powder, particle, and straws, respectively, which provided a recycle protocol to convert polylactic acid waste into value-added chemicals.
Synthesis, Thermal and Mechanical Properties of Fully Biobased Poly (hexamethylene succinate-co-2,5-furandicarboxylate) Copolyesters
Authors: Wang Chengqian , Chen Mingkun , Jiang Zhiguo , Qiu Zhaobin
Published: January 2023

Poly (hexamethylene succinate) (PHS) is a biobased and biodegradable polyester. In this research, two fully biobased high-molecular-weight poly (hexamethylene succinate-co-2,5-furandicarboxylate) (PHSF) copolyesters with low hexamethylene furandicarboxylate (HF) unit contents (about 5 and 10 mol%) were successfully synthesized through a two-step transesterification/esterification and polycondensation method. The basic thermal behavior, crystal structure, isothermal crystallization kinetics, melting behavior, thermal stability, and tensile mechanical property of PHSF copolyesters were studied in detail and compared with those of PHS. PHSF showed a decrease in the melt crystallization temperature, melting temperature, and equilibrium melting temperature while showing a slight increase in the glass transition temperature and thermal decomposition temperature. PHSF copolyesters displayed the same crystal structure as PHS. Compared with PHS, PHSF copolyesters showed the improved mechanical property. The presence of about 10 mol% of HF unit increased the tensile strength from 12.9 ± 0.9 MPa for PHS to 39.2 ± 0.8 MPa; meanwhile, the elongation at break also increased from 498.5 ± 4.78% to 1757.6 ± 6.1%.
Multiple Self-Healing Effects of Water-Absorbing Microcapsules in Cementitious Materials
Authors: Mao Qianjin , Chen Jiayi , Wu Wenwen , Li Runfeng , Shi Shuqing , Wang Ziming , Cui Suping
Published: January 2023

Concrete cracking has a negative impact on the durability of the structure. Pre-implanting microcapsules containing healing agents into the concrete are expected to induce the cracks to self-heal. However, the self-healing effect can potentially be influenced by several environmental conditions, thus limiting its applications. To address these challenges, we developed a new type of water-absorbing microcapsules, using calcium alginate hydrogel as the wall material and an adhesive epoxy polymer as the core material, to improve the self-healing adaptability in complex and changing environments. We explored the healing properties and mechanism of cementitious materials containing microcapsules under various environmental conditions. The experimental results showed that the water-absorbent microcapsules exhibit multiple self-healing effects under different external conditions: (1) in an anhydrous environment, fissures prompted the activation of microcapsules, and the epoxy polymer flowed out to seal the cracks. (2) When exposed to water, the microcapsules inflated to form a seal around the fissures. (3) The microcapsules facilitated the autogenous healing of cracks in the cementitious material when wet and dry conditions were alternated. The three self-healing mechanisms worked synergistically and contributed to the effective restoration of the impermeability and strength of concrete under different environments. Particularly, the recovery of compressive strength and impermeability exceeded 100% when the microcapsule content was 4% and the pre-pressure was 40% of fmax.
Physico-Chemical Characterization of Food Wastes for Potential Soil Application
Authors: Islam Md. Rezwanul , Wang Qingyue , Guo Yue , Wang Weiqian , Sharmin Sumaya , Ebere Enyoh Christian
Published: January 2023

Food waste is a matter of deep concern as it is creating multidimensional threats to the environment. Though these are potential sources of plant nutrients, a detailed investigation has not yet been conducted. Hence, a physico-chemical characterization of non-edible food waste such as brinjal waste, potato peels, banana peels, orange peels, eggshells, cow bones, chicken bones, fish bones, and their combined waste was accomplished using FTIR, TGA, XGT-5000, CHN corder, etc. The present study revealed that plant waste samples had lower ash contents (1.36–7.79%) but higher volatile matter (72.33–80.04%), and the reverse was true in animal waste. In addition, animal waste, except eggshells (22.48%), had lower fixed carbon (1.01–2.83%). On the other hand, fruit waste was documented with higher fixed carbon (11.33–14.27%). Results also indicated that animal waste and their combined samples, except eggshells, contained more nitrogen (3.82–5.06%). The concentration of P was statistically significant in the combined fruit sample (28.21% ± 8.451), followed by banana peels (24.96% ± 11.644). Vegetable waste contained a significantly higher amount of K (24.90% ± 28.311–37.92% ± 24.833) than other waste. Maximum Ca (68.82% ± 10.778) and Mg (15.37% ± 29.88) were recorded in eggshell and fish bone waste, respectively. Except for Zn and Mn in eggshells, the waste studied contained a significant amount of micronutrients. FTIR demonstrated the presence of different functional groups such as alcoholic group (OH), aliphatic chain (C–H), aromatic chain (C–H), alkyl aryl ether (C–O), carbonyl group (C=O), alkene (C=C), thiocyanate (S–CN), isothiocyanate (N=C=S), allene (C=C=C), sulphate (S=O), amine (N–H), and NO stretching band, which varied with the waste used. Thermogravimetric analysis revealed that plant waste degraded more quickly than animal waste. Eggshells were found to be less degradable by increased temperature, followed by cow bones > fish bones > chicken bones. The waste material used in the study was slightly acidic (6.67–6.82) in nature. Correlation analysis of the nutrients showed mainly positive relations in all samples for macro and micronutrients. Therefore, non-edible food waste can be a possible source of plant nutrients.
Influence of Water on the Production of Liquid Fuel Intermediates from Furfural via Aldol Condensation over MgAl Catalyst
Authors: Tišler Zdeněk , Vondrová Pavla , Peroutková Kateřina , Šimek Josef , Skuhrovcová Lenka , Strejcová Kateřina , Svobodová Eliška , Gholami Zahra
Published: January 2023

The aldol condensation of furfural and acetone is considered a promising method for the production of liquid fuel intermediates. 4-(2-furyl)-3-buten-2-one (FAc) and 1,5-di-2-furanyl-1,4-pentadien-3-one (F2Ac) are the main products of the reaction, which can go through the hydrodeoxygenation process to convert to diesel and jet fuel range fuels. Considering the present situation at the fuel-market related to crude oil shortage, the above-mentioned process seems to be a convenient path to obtain fuels in the diesel and kerosene range. This research focuses on the effect of water on the furfural conversion and product distribution during the aldol condensation. The catalyst chosen for this research was MgAl mixed oxide in molar ratio 3:1. The reaction was performed at 40 °C and 1 MPa in a continuous-flow reactor with and without water in the feedstock. The physicochemical properties of the catalyst were evaluated using different techniques. The catalyst lifetime decreased and the catalyst deactivation started faster by the addition of 5 wt.% water to the feedstock with the furfural to acetone ratio (F:Ac) of 1:2.5. Selectivity to FAc increased by 10% in the presence of water. The catalyst lifetime enhanced by increasing the F:Ac ratio from 1:2.5 to 1:5, in the presence of 5 wt.% water. The furfural conversion was 100% after 28 h of reaction, and then decreased gradually to 40% after 94 h of reaction. At higher F:Ac ratio, the selectivity to FAc was 10% higher, while the F2Ac was about 8% lower.
ICESat-2 Bathymetric Signal Reconstruction Method Based on a Deep Learning Model with Active–Passive Data Fusion
Authors: Leng Zihao , Zhang Jie , Ma Yi , Zhang Jingyu
Published: January 2023

When carrying out SDB (satellite-derived bathymetry) in island area based on ICESat-2 (Ice, Cloud, and land Elevation Satellite 2) data, it is often found that the ICESat-2 bathymetric signals are partially missing due to the influence of thick aerosols such as clouds and fog. This not only hinders the accurate extraction of the along-track underwater topography, but also restricts the active–passive fusion bathymetry based on ICESat-2 data and multi/hyperspectral remote sensing images. In this paper, aiming at the partially missing ICESat-2 bathymetric signals, combined with passive optical remote sensing images, and based on an LSTM (long short-term memory) deep recurrent neural network model, an ICESat-2 bathymetric signal reconstruction method based on active–passive data fusion is proposed. It is found that this method can effectively reconstruct the local missing bathymetric signals. When the reconstructed ICESat-2 bathymetric data are applied to carry out active–passive fusion and bathymetric inversion, the accuracy indices are better than those of the inversion results of the data with partial missing signals, and the performance is comparable to that of the original data without missing data, which is of great value for the bathymetric application of ICESat-2 data in island and reef areas.