Influence of Inositol on The Content of Oxidatively Modified Proteins and Morphological Changes in the Skin of Animals of Different Sexes with Metabolic Associated Fatty Liver Disease
Authors: Sopel Olesya , Denefil Olha 
Published: December 2024

Introduction. Metabolic dysfunction-associated steatotic liver disease (MASLD) remains a leading cause of chronic liver disease worldwide. Data on the global epidemic of obesity are driving a number of obesity-related complications, including MASLD, and it present in 20-30% in the European Union, and from 15% to 40% in Asia, of 30% of Middle East and Turkey, 13.5% in Africa countries. MASLD is also complicated by other accompanying metabolic diseases, and changes occur in other organs, including the heart, eyes, and kidneys. At skin, there is not a lot of investigation of pathogenetic processes in the MASLD. As a cause of violation of the integrity of cells we can determine the oxidative stress, and disfunction of cells membranes, lipids and proteins. There are data in the literature that inositols normalize lipid metabolism in the liver, so it is reasonable to study their effect on the skin.
The aim of the research: determine the features of changes in the content of oxidative modified proteins in the homogenates of the skin of rats with experimentally simulated metabolically associated fatty liver disease and the influence of inositol isoforms on their content and to evaluate the morphological changes in the skin under these conditions.
Research methods. Experiments were performed on 216 white rats of different sexes, at the time of withdrawal from the experiment they were 7.5-8 months old. The animals were divided into 9 groups: 1 – control, 2 – myo-inositol (MI), 3 – D-chiro inositol (DI), 4 – MASLD 2 months, 5 – MASLD 2 months + MI 2 months, 6 – MASLD 2 months + DI 2 months, 7 – MASLD 4 months, 8 – MASLD 4 months+ MI 2 last months, 9 – MASLD 4 months+DI 2 last months. MASLD was induced by giving 20% fructose solution (Fr) instead of drinking for 2 months in groups 4. 5 and 6, for 4 months in groups 7, 8 and 9. Inositols were administered intragastrically (MI – 400 mg/kg of weight, DI – 30 mg/kg of weight) after 2 months from the beginning of the simulation of MASLD. The content of oxidative modified proteins (OMP370, OMP430) was determined in eye homogenates. A morphological study of the skin was carried out.
Results and discussion. Modeling of MASLD, even 2 months after cessation of fructose consumption, caused an increase in males OMP370 by 3.4 times (p<0.001), OMP430 – by 3.5 times (p<0.001); in females OMP370 increased by 3.3 times (p<0.001), OMP430 – by 3.3 times (p<0.01). Simulation of MASLD during 4 months of fructose consumption caused an increase in males OMP370 by 6.3 times (p<0.001), OMP430 – by 7.2 times (p<0.001); in females OMP370 increased by 5.4 times (p<0.001), OMP430 – by 6.9 times (p<0.001). MI and DI decreased the OMP level. The morphological study causes significant disturbance of the skin layers of animals. Using of MI and DI caused much smaller changes, and more expressed effect has MI.
Conclusions. 1. In experimentally modeled MASLD, OMP accumulate in the skin of male and female rats. Longer consumption of Fr causes more damage. 2. Correction of simulated MASLD with MI and DI during 2 and 4 months fructose consumption causes a decrease in the OMP in skin homogenates, which is more pronounced during 4 months Fr consumption in males, and with 2 months use – in females. 3. The morphological study of the skin under the influences of drinking a Fr solution for 2 and 4 months causes significant disturbance of the skin layers of animals. MI and DI in MASLD caused much smaller changes, and more expressed effect has MI.