bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Evidence of Orthomyxovirus Presence in Colombian Neotropical Bats
Authors: Uribe Manuel , Rodríguez-Posada Miguel E. , Ramirez-Nieto Gloria C.
Published: April 2022

The Orthomyxoviridae family includes the genera Influenzavirus, Isavirus, Quaranjavirus, and Thogotovirus. In turn, Influenzavirus can be classified into four types: α, β, γ, and δ (Formerly A, B, C, and D), from which Alphainfluenzavirus (AIV) has the broadest host range, including birds, mammals, reptiles, and amphibians. Additionally, AIV has shown global epidemiological relevance owing to its pandemic potential. The epidemiological relevance of Chiropteran due to its multiple functional characteristics makes them ideal reservoirs for many viral agents. Recently, new influenza-like subtypes have been reported in Neotropical bats, but little is known about the relevance of bats as natural reservoirs of influenza viruses. Therefore, the current study aimed to determine the presence of AIV and new influenza-like subtypes in South American bats. For a better understanding of the drivers and interactions between AIV and bats, we used molecular assays with different gene targets (i.e., M, NP, and PB1) to identify AIV in New World bats. A housekeeping gene (CytB) PCR was used to check for nucleic acid preservation and to demonstrate the bat-origin of the samples. A total of 87 free-living bats belonging to 25 different species of the families Phyllostomidae and Vespertilionidae were collected in Casanare, Colombia. As a result, this study found seven AIV-positive bat species, three of them reported for the first time as AIV prone hosts. Neither of the AIV-like analyzed samples were positive for H17N10/H18/N11subtypes. Although additional information is needed, the presence of a completely new or divergent AIV subtype in neotropical bats cannot be discarded. Collectively, the results presented here expand the epidemiological knowledge and distribution of AIV in neotropical free-ranging bats and emphasize the need to continue studying these viruses to establish the role they could play as a threat to animal and public health.
Lactobacillus delbrueckii CIDCA 133 Ameliorates Chemotherapy-Induced Mucositis by Modulating Epithelial Barrier and TLR2/4/Myd88/NF-κB Signaling Pathway
Authors: Barroso Fernanda Alvarenga Lima , de Jesus Luís Cláudio Lima , da Silva Tales Fernando , Batista Viviane Lima , Laguna Juliana , Coelho-Rocha Nina Dias , Vital Kátia Duarte , Fernandes Simone Odília Antunes , Cardoso Valbert Nascimento , Ferreira Enio , Martins Flaviano Santos , Drumond Mariana Martins , Mancha-Agresti Pamela , Birbrair Alexander , Barh Debmalya , Azevedo Vasco
Published: April 2022

Intestinal mucositis promoted by the use of anticancer drugs is characterized by ulcerative inflammation of the intestinal mucosa, a debilitating side effect in cancer patients undergoing treatment. Probiotics are a potential therapeutic option to alleviate intestinal mucositis due to their effects on epithelial barrier integrity and anti-inflammatory modulation. This study investigated the health-promoting impact of Lactobacillus delbrueckii CIDCA 133 in modulating inflammatory and epithelial barrier markers to protect the intestinal mucosa from 5-fluorouracil-induced epithelial damage. L. delbrueckii CIDCA 133 consumption ameliorated small intestine shortening, inflammatory cell infiltration, intestinal permeability, villus atrophy, and goblet cell count, improving the intestinal mucosa architecture and its function in treated mice. Upregulation of Muc2, Cldn1, Hp, F11r, and Il10, and downregulation of markers involved in NF-κB signaling pathway activation (Tlr2, Tlr4, Nfkb1, Il6, and Il1b) were observed at the mRNA level. This work suggests a beneficial role of L. delbrueckii strain CIDCA 133 on intestinal damage induced by 5-FU chemotherapy through modulation of inflammatory pathways and improvement of epithelial barrier function.
Use of Fourier-Transform Infrared Spectroscopy With IR Biotyper® System for Legionella pneumophila Serogroups Identification
Authors: Pascale Maria Rosaria , Bisognin Francesco , Mazzotta Marta , Girolamini Luna , Marino Federica , Dal Monte Paola , Cordovana Miriam , Scaturro Maria , Ricci Maria Luisa , Cristino Sandra
Published: April 2022

Legionella spp. are Gram-negative bacteria that inhabit freshwater environments representing a serious risk for human health. Legionella pneumophila (Lp) is the species most frequently responsible for a severe pneumonia known as Legionnaires' disease. Lp consists of 15 serogroups (Sgs), usually identified by monoclonal or polyclonal antibodies. With regard to Lp serogrouping, it is well known that phenotyping methods do not have a sufficiently high discriminating power, while genotypic methods although very effective, are expensive and laborious. Recently, mass spectrometry and infrared spectroscopy have proved to be rapid and successful approaches for the microbial identification and typing. Different biomolecules (e.g., lipopolysaccharides) adsorb infrared radiation originating from a specific microbial fingerprint. The development of a classification system based on the intra-species identification features allows a rapid and reliable typing of strains for diagnostic and epidemiological purposes. The aim of the study was the evaluation of Fourier Transform Infrared Spectroscopy using the IR Biotyper® system (Bruker Daltonik, Germany) for the identification of Lp at the serogroup (Sg) level for diagnostic purposes as well as in outbreak events. A large dataset of Lp isolates (n = 133) and ATCC reference strains representing the 15 Lp serogroups were included. The discriminatory power of the instrument's classifier, was tested by Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA). All isolates were classified as follows: 12/133 (9.0%) as Lp Sg1 and 115/133 (86.5%) as Lp Sg 2–15 (including both ATCC and environmental Lp serogroup). Moreover, a mis-classification for 2/133 (1.5%) isolates of Lp Sg 2–15 that returned as Lp Sg1 was observed, and 4/133 (3.0%) isolates were not classified. An accuracy of 95.49% and an error rate of 4.51% were calculated. IR Biotyper® is able provide a quick and cost-effective reliable Lp classification with advantages compared with agglutination tests that show ambiguous and unspecific results. Further studies including a larger number of isolates could be useful to implement the classifier obtaining a robust and reliable tool for the routine Lp serogrouping. IR Biotyper® could be a powerful and easy-to-use tool to identify Lp Sgs, especially during cluster/outbreak investigations, to trace the source of the infection and promptly adopt preventive and control strategies.
Bacteriocin-Like Inhibitory Substances in Staphylococci of Different Origins and Species With Activity Against Relevant Pathogens
Authors: Fernández-Fernández Rosa , Lozano Carmen , Eguizábal Paula , Ruiz-Ripa Laura , Martínez-Álvarez Sandra , Abdullahi Idris Nasir , Zarazaga Myriam , Torres Carmen
Published: April 2022

Bacteriocins are antimicrobial peptides with relevance in the modulation of human and animal microbiota that have gained interest in biomedical and biotechnological applications. In this study, the production of bacteriocin-like inhibitory substances (BLIS) was tested among a collection of 890 staphylococci of different origins (humans, animals, food, and the environment) and species, both coagulase-positive (CoPS, 238 isolates of 3 species) and coagulase-negative staphylococci (CoNS, 652 isolates of 26 species). Of the 890 staphylococci, 60 (6.7%) showed antimicrobial activity by the spot-on-lawn method against at least one of the 25 indicator bacteria tested. BLIS-producer (BLIS+) isolates were detected in 8.8% of CoPS and 6.0% of CoNS. The staphylococcal species with the highest percentages of BLIS+ isolates were S. chromogenes (38.5%), S. pseudintermedius (26.7%), and S. warneri (23.1%). The production of BLIS was more frequently detected among isolates of pets, wild animals, and food. Moreover, 13 BLIS+ isolates showed wide antimicrobial activiy spectrum, and 7 of these isolates (of species S. aureus, S. pseudintermedius, S. sciuri, and S. hominis) demonstrated antimicrobial activity against more than 70% of the indicator bacteria tested. The genetic characterization (by PCR and sequencing) of the 60 BLIS+ isolates revealed the detection of (a) 11 CoNS and CoPS isolates carrying putative lantibiotic-like genes; (b) 3 S. pseudintermedius isolates harboring the genes of BacSp222 bacteriocin; and (c) 2 S. chromogenes isolates that presented the gene of a putative cyclic bacteriocin (uberolysin-like), being the first report in this CoNS species. Antimicrobial susceptibility testing was performed in BLIS+ isolates and one-third of the CoNS isolates showed susceptibility to all antibiotics tested, which also lacked the virulence genes studied. These BLIS+ CoNS are good candidates for further characterization studies.
A State-of-the-Art Review of EEG-Based Imagined Speech Decoding
Authors: Lopez-Bernal Diego , Balderas David , Ponce Pedro , Molina Arturo
Published: April 2022

Currently, the most used method to measure brain activity under a non-invasive procedure is the electroencephalogram (EEG). This is because of its high temporal resolution, ease of use, and safety. These signals can be used under a Brain Computer Interface (BCI) framework, which can be implemented to provide a new communication channel to people that are unable to speak due to motor disabilities or other neurological diseases. Nevertheless, EEG-based BCI systems have presented challenges to be implemented in real life situations for imagined speech recognition due to the difficulty to interpret EEG signals because of their low signal-to-noise ratio (SNR). As consequence, in order to help the researcher make a wise decision when approaching this problem, we offer a review article that sums the main findings of the most relevant studies on this subject since 2009. This review focuses mainly on the pre-processing, feature extraction, and classification techniques used by several authors, as well as the target vocabulary. Furthermore, we propose ideas that may be useful for future work in order to achieve a practical application of EEG-based BCI systems toward imagined speech decoding.
ACU193: An Immunotherapeutic Poised to Test the Amyloid β Oligomer Hypothesis of Alzheimer’s Disease
Authors: Krafft Grant A. , Jerecic Jasna , Siemers Eric , Cline Erika N.
Published: April 2022

Alzheimer’s disease (AD) is an age-related neurodegenerative disease that affects 50 million people worldwide, with 10 million new cases occurring each year. The emotional and economic impacts of AD on patients and families are devastating. Approved treatments confer modest improvement in symptoms, and recently one treatment obtained accelerated approval from the United States Food and Drug Administration (FDA) and may have modest disease modifying benefit. Research over the past three decades has established a clear causal linkage between AD and elevated brain levels of amyloid β (Aβ) peptide, and substantial evidence now implicates soluble, non-fibrillar Aβ oligomers (AβOs) as the molecular assemblies directly responsible for AD-associated memory and cognitive failure and accompanying progressive neurodegeneration. The widely recognized linkage of elevated Aβ and AD spawned a comprehensive 20-year therapeutic campaign that focused primarily on two strategies – inhibition of the secretase enzymes responsible for Aβ production and clearance of Aβ peptide or amyloid plaques with Aβ-directed immunotherapeutics. Unfortunately, all clinical trials of secretase inhibitors were unsuccessful. Of the completed phase 3 immunotherapy programs, bapineuzumab (targeting amyloid plaque) and solanezumab (targeting Aβ monomers) were negative, and the crenezumab program (targeting Aβ monomers and to a small extent oligomers) was stopped for futility. Aducanumab (targeting amyloid plaques), which recently received FDA accelerated approval, had one positive and one negative phase 3 trial. More than 25 negative randomized clinical trials (RCTs) have evaluated Aβ-targeting therapeutics, yet none has directly evaluated whether selective blockage of disease-relevant AβOs can stop or reverse AD-associated cognitive decline. Here, we briefly summarize studies that establish the AD therapeutic rationale to target AβOs selectively, and we describe ACU193, the first AβO-selective immunotherapeutic to enter human clinical trials and the first positioned to test the AβO hypothesis of AD.
A Cross-Sectional Study of Breast Cancer Surgery and the Cost Based on Data From 77 Chinese 3A Hospitals in 2015
Authors: Zhao Rong , Jin Meng , Gao Jinnan , Zhang Linhui , Tao Liyuan , Bao Xiaoyuan
Published: April 2022

Purposes

We aimed to clarify the real-world status of breast cancer surgery and the cost in China.

Methods

This cross-sectional survey relied on data obtained from the hospitalization summary reports (HSRs) in 77 top-ranked (grade 3A) hospitals in China to analyze breast cancer patients who underwent surgery between January 2015 and December 2015. The surgery and cost were mainly evaluated.

Results

Overall, 31,900 breast cancer patients underwent surgeries in 77 hospitals. The mean age in our study was 51.5 years (SD, 11.7 years). The primary types of surgical procedures were mastectomy (n = 24,629, 77.2%) and breast-conserving surgery (6,210, 19.5%). The rate of mastectomy was the highest at age band 50–65 years (n = 10,861, 82.1%) and in non-first-tier cities (n = 7,651, 88.4%) as well as in Northeast China (n = 3,107, 93.2%). The rate of breast-conserving surgery was less than 10% in non-first-tier cities (9.8%), Southwest China (6.1%), and Northeast China (5.8%). The median cost was $3,352.4 (interquartile range (IQR), $2,492.6–4,588.0). Mastectomy cost was significantly higher than breast-conserving surgery cost in both different city tiers and regional distribution except Northeast China (p < 0.001).

Conclusions

This study demonstrated that the main breast cancer surgery in Chinese 3A hospitals was mastectomy and that the cost varied both across and within geographic regions and city tiers. This information helps describe the real-world status of breast surgery and the cost in China.
Histone Acetylation and Modifiers in Renal Fibrosis
Authors: Shen Fengchen , Zhuang Shougang
Published: April 2022

Histones are the most abundant proteins bound to DNA in eukaryotic cells and frequently subjected to post-modifications such as acetylation, methylation, phosphorylation and ubiquitination. Many studies have shown that histone modifications, especially histone acetylation, play an important role in the development and progression of renal fibrosis. Histone acetylation is regulated by three families of proteins, including histone acetyltransferases (HATs), histone deacetylases (HDACs) and bromodomain and extraterminal (BET) proteins. These acetylation modifiers are involved in a variety of pathophysiological processes leading to the development of renal fibrosis, including partial epithelial-mesenchymal transition, renal fibroblast activation, inflammatory response, and the expression of pro-fibrosis factors. In this review, we summarize the role and regulatory mechanisms of HATs, HDACs and BET proteins in renal fibrosis and provide evidence for targeting these modifiers to treat various chronic fibrotic kidney diseases in animal models.
Magnetic Resonance Imaging Investigation of Neuroplasticity After Ischemic Stroke in Tetramethylpyrazine-Treated Rats
Authors: Feng Xue-Feng , Lei Jian-Feng , Li Man-Zhong , Zhan Yu , Yang Le , Lu Yun , Li Ming-Cong , Zhuang Yu-Ming , Wang Lei , Zhao Hui
Published: April 2022

Ischemic stroke elicits white matter injury typically signed by axonal disintegration and demyelination; thus, the development of white matter reorganization is needed. 2,3,5,6-Tetramethylpyrazine (TMP) is widely used to treat ischemic stroke. This study was aimed to investigate whether TMP could protect the white matter and promote axonal repair after cerebral ischemia. Male Sprague–Dawley rats were subjected to permanent middle cerebral artery occlusion (MCAO) and treated with TMP (10, 20, 40 mg/kg) intraperitoneally for 14 days. The motor function related to gait was evaluated by the gait analysis system. Multiparametric magnetic resonance imaging (MRI) was conducted to noninvasively identify gray-white matter structural integrity, axonal reorganization, and cerebral blood flow (CBF), followed by histological analysis. The expressions of axonal growth-associated protein 43 (GAP-43), synaptophysin (SYN), axonal growth-inhibitory signals, and guidance factors were measured by Western blot. Our results showed TMP reduced infarct volume, relieved gray-white matter damage, promoted axonal remodeling, and restored CBF along the peri-infarct cortex, external capsule, and internal capsule. These MRI findings were confirmed by histopathological data. Moreover, motor function, especially gait impairment, was improved by TMP treatment. Notably, TMP upregulated GAP-43 and SYN and enhanced axonal guidance cues such as Netrin-1/DCC and Slit-2/Robo-1 but downregulated intrinsic growth-inhibitory signals NogoA/NgR/RhoA/ROCK-2. Taken together, our data indicated that TMP facilitated poststroke axonal remodeling and motor functional recovery. Moreover, our findings suggested that TMP restored local CBF, augmented guidance cues, and restrained intrinsic growth-inhibitory signals, all of which might improve the intracerebral microenvironment of ischemic areas and then benefit white matter remodeling.
Tanhuo Formula Inhibits Astrocyte Activation and Apoptosis in Acute Ischemic Stroke
Authors: Nie Yuting , Wen Lulu , Li Hui , Song Juexian , Wang Ningqun , Huang Liyuan , Gao Li , Qu Miao
Published: April 2022

Tanhuo formula (THF), a traditional Chinese medicinal formula, has been demonstrated to be effective in the clinical treatment of acute ischemic stroke (AIS). However, its active ingredients, potential targets, and molecular mechanisms remain unknown. Based on the validation of active ingredient concentrations, our study attempted to elucidate the possible mechanisms of THF based on network pharmacological analysis and experimental validation. Components of THF were screened using network pharmacological analysis, and a compound–target network and protein–protein interaction (PPI) network were constructed. In total, 42 bioactive compounds and 159 THF targets related to AIS were identified. The PPI network identified AKT1, TNF, IL6, IL1B, and CASP3 as key targets. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis demonstrated that the inflammation and apoptotic pathways were enriched by multiple targets. The main components of THF were identified via high-performance liquid chromatography. Subsequently, a validation experiment was conducted, and the expressions of GFAP, C3, TNF-α, and IL-6 were detected via immunofluorescence staining, confirming the inflammatory response at 30 min and 3 days post injury. Immunohistochemical staining for caspase-3 and TUNEL was also performed to assess apoptosis at the same time points. These results indicate that THF can effectively decrease neural cell apoptosis through the caspase-3 pathway and restrain excessive abnormal activation of astrocytes and the release of TNF-α and IL-6, which might be accompanied by the recovery of motor function. Thus, THF may serve as a promising therapeutic strategy for AIS through multiple targets, components, and pathways.
Dicoma anomala Enhances Phthalocyanine Mediated Photodynamic Therapy in MCF-7 Breast Cancer Cells
Authors: Chota Alexander , George Blassan P. , Abrahamse Heidi
Published: April 2022

Breast cancer is one of the most common types of cancer in women, and it is regarded as the second leading cause of cancer-related deaths worldwide. The present study investigated phytochemical profiling, in vitro anticancer effects of Dicoma anomala methanol root extract and its enhancing effects in phthalocyanine mediated PDT on MCF-7 (ATCC® HTB-22™) breast cancer cells. Ultra-high performance liquid chromatography coupled to electrospray ionization quadrupole-time of flight mass spectrometry (UHPLC-qTOF-MS2) was used to identify the secondary metabolites in the crude extract. The 50% inhibitory concentration (IC50) of the two experimental models was established from dose response studies 24 h post-treatment with D. anomala methanol root extract (25, 50, and 100 μg/ml) and ZnPcS4 (5, 10, 20, 40, and 60 μM) mediated PDT. The inverted microscope was used to analyze morphological changes, trypan blue exclusion assay for viability, and Annexin V-fluorescein isothiocyanate (FITC)-propidium iodide (PI) for cell death mechanisms. Immunofluorescence analysis was used to investigate the qualitative expression of the Bax, p53, and caspase 3 apoptotic proteins. Experiments were performed 4 times (n = 4) and SPSS version 27 software was used to analyze statistical significances. D. anomala methanol root extract induced cell death in MCF-7 cells by decreasing cell viability. The combination of D. anomala methanol root extract and ZnPcS4 mediated PDT led to a significant increase in apoptotic activities, expression of Bax, and p53 with significant decrease in cell viability. These findings pinpoint the possibility of D. anomala methanol root extract of being employed as a natural antiproliferative agent in the treatment of various cancers.
The Effect of Dietary Fat and Sucrose on Cognitive Functioning in Mice Lacking Insulin Signaling in Neuropeptide Y Neurons
Authors: Mitchell Caitlin S. , Goodman Elisabeth K. , Tedesco Caitlin R. , Nguyen Kathy , Zhang Lei , Herzog Herbert , Begg Denovan P.
Published: April 2022

Obesogenic diets can produce hippocampal insulin resistance and impairments to hippocampal-dependent cognition. This study investigated the effect of disrupted insulin signaling in Neuropeptide Y (NPY) neurons on diet-induced deficits in hippocampal-dependent memory. Wild-type mice and mice that had a targeted knockout of insulin receptors on NPY cells (IRlox/lox;NPYCre/+) were given ad libitum access to a high-fat diet (high fat; HF), 10% sucrose solution (high sugar; HS), both high-fat diet and sucrose solution (high fat, high sugar; HFHS), or a normal fat control chow for 12 weeks. Mice were tested in the Morris Water Maze (MWM), a hippocampal-dependent spatial memory task. Glucose homeostasis was assessed via a glucose tolerance test. Independent of genotype, consumption of HF, but not HS, diet increased energy intake, body weight, and plasma leptin, and impaired glucose tolerance. Disrupted insulin signaling in NPY cells and dietary interventions did not significantly affect the ability of mice to learn the location of the platform in the MWM. However, for IRlox/lox control mice, consumption of HF, but not HS, diet resulted in reduced time spent in the target quadrant during the probe trial, suggesting a hippocampal-dependent memory deficit. IRlox/lox;NPYCre/+ mice had poor performance in the probe trial regardless of diet, suggesting a floor effect. This study did not find adverse effects of chronic sucrose intake on metabolic outcomes or hippocampal-dependent memory. These data also suggest that the effects of HF diet on hippocampal-dependent memory may be dependent on insulin signaling in hippocampal NPY cells.
A Parameter Representing Missing Charge Should Be Considered when Calibrating Action Potential Models
Authors: Barral Yann-Stanislas H. M. , Shuttleworth Joseph G. , Clerx Michael , Whittaker Dominic G. , Wang Ken , Polonchuk Liudmila , Gavaghan David J. , Mirams Gary R.
Published: April 2022

Computational models of the electrical potential across a cell membrane are longstanding and vital tools in electrophysiology research and applications. These models describe how ionic currents, internal fluxes, and buffering interact to determine membrane voltage and form action potentials (APs). Although this relationship is usually expressed as a differential equation, previous studies have shown it can be rewritten in an algebraic form, allowing direct calculation of membrane voltage. Rewriting in this form requires the introduction of a new parameter, called Γ0 in this manuscript, which represents the net concentration of all charges that influence membrane voltage but are not considered in the model. Although several studies have examined the impact of Γ0 on long-term stability and drift in model predictions, there has been little examination of its effects on model predictions, particularly when a model is refit to new data. In this study, we illustrate how Γ0 affects important physiological properties such as action potential duration restitution, and examine the effects of (in)correctly specifying Γ0 during model calibration. We show that, although physiologically plausible, the range of concentrations used in popular models leads to orders of magnitude differences in Γ0, which can lead to very different model predictions. In model calibration, we find that using an incorrect value of Γ0 can lead to biased estimates of the inferred parameters, but that the predictive power of these models can be restored by fitting Γ0 as a separate parameter. These results show the value of making Γ0 explicit in model formulations, as it forces modellers and experimenters to consider the effects of uncertainty and potential discrepancy in initial concentrations upon model predictions.
Lockdown in France: Impact on Families of Young Children With Special Needs
Authors: Pinel-Jacquemin Stéphanie , Martinez Amalia , Martinasso Maud , Katkoff Valerie , Willig Thiébaut-Noël , Zaouche Gaudron Chantal
Published: April 2022

Background

Families with young children have faced serious challenges during the first lockdown as a result of the COVID-19 pandemic. In addition to remote working, parents have had to monitor their children’s schoolwork and manage their daily lives. When one of the children also has neuro-developmental disorders, this results in an increased burden. We can therefore wonder how these families with one or more young children (under 6 years old) with special needs have experienced and dealt with this lockdown.

Aim of the Study

In this context, the “COVJEUNENFANT” study focused more specifically on the subjective experience, as a parent, of those who cared for children with special needs (i.e., with developmental disorders, neurodevelopmental disorders, proven disabilities or chronic health conditions) compared to the general population. We wished to see if the consequences of the health crisis were significantly different from those perceived by respondents in the general population (n = 490) and if the sociodemographic structure of these families differed from those of other respondents.

Methods

Ninety three French families with at least one child under 6 years old and one with developmental difficulties or a chronic illness, from a cohort of 490 control families, participated in a web-based survey during the first lockdown, from the 28th April 2020 to 29th May 2020.

Results

After presenting the participants’ sociodemographic characteristics, the results show that these French families (n = 93) are less wealthy than the control population “without special needs” (n = 397), have felt more pressures originating from their environment (families, friends, colleagues, media, social networks…), have suffered from more health issues (other than COVID-19), have taken more measures to protect themselves (social-distancing), and were less likely to feel happy. A significantly larger number of them lamented the lack of free time and voiced a larger need for information regarding children’s education. However, their parental role was felt as being more satisfying and their family relations strengthened more than in the general population of participants.

Conclusion

It is apparent that urgent prioritisation is needed in order to support and care for these families by continuing to provide care for their children in one way or another, and by ensuring that their need to adapt again does not exceed their own abilities and resources, especially as young children, who have high levels of requirements, are present in the home.
Comparisons Between COVID-19 Stigma and Other Stigmas: Distinct in Explicit Attitudes and Similar in Implicit Process
Authors: Zhu Jiajia , Yan Lihua , Mu Yan
Published: April 2022

Since the outbreak of COVID-19, the public stigma associated with COVID-19 has emerged. To better understand the COVID-19 stigma, the present research conducted three studies on 1,493 Chinese participants from the outbreak to the recovery period of the COVID-19 pandemic to examine the psychological mechanisms of COVID-19 stigma by comparing it with other disease-related stigmas in terms of their explicit and implicit processes. Study 1 and Study 2 jointly demonstrated that the public endorsed more stigma toward the COVID-19 related people (i.e., the COVID-19 patients) relative to the other disease-related people (i.e., the SARS patients, people with flu) in multiple explicit aspects, including emotional, motivational, cognitive, and social processing. Using the implicit association test (IAT), Study 3 found no significant difference in the implicit measures of the COVID-19 vs. the SARS groups, which further revealed that the pandemic stigmas (i.e., COVID-19 and SARS) were similar at the implicit level. These findings suggest common (implicit level) but distinct (explicit level) psychological processes of the pandemic-related stigmas, which provide reference to policymakers in formulating suitable interventions to deal with COVID-19 stigma and a newly generated potential stigma and provide psychological support for the public in the future.
Accessing the Influence of Community Experience on Brand Loyalty Toward Virtual Brand Community: Developing Country Perspective
Authors: Huangfu Zhounan , Ruan Yaohui , Zhao Jing , Wang Qiaqia , Zhou Lei
Published: April 2022

With the development of information technology, more and more companies have taken the initiative to build virtual brand communities to strengthen the connection between brands and customers and create brand loyalty, but existing research lacks a clear explanation of how the customer community experience of virtual brand communities affects brand loyalty. In response to this question, this paper investigates the role and mechanism of different types of community experiences, namely information experience, entertainment experience and interactive experience, generated by customers’ participation in community activities on brand loyalty based on the customer experience perspective. The findings show that different types of community experiences have a significant impact on brand loyalty, and which community identity is partially mediated in all of them. Further analysis for segmented populations in virtual communities revealed significant differences in the above mechanisms for members of different genders. The findings of this paper are important guidance for companies to manage virtual brand communities and create brand loyalty.
Merger and Acquisitions Integration, Implementation as Innovative Approach Toward Sustainable Competitive Advantage: A Case Analysis From Chinese Sports Brands
Authors: Chiu Wen-Hong , Shih Yuan-Shen , Chu Li-Sheng , Chen Shieh-Liang
Published: April 2022

Brand M&A has long been an extremely common strategy for expanding the scale of an organization and entering new business areas, but various signs show that many brand mergers and acquisitions (M&As) do not add value. They often lose money and fail. This research explores the value, scarcity, and non-replicability of resources in corporate M&A, as well as organizational resource management, innovation resource management, and implementation of the combination of resource utilization and brand strategy that incorporate M&As. Taking 03 of China’s sporting shoe industry cases, this study uses the literature to collect, analyze, and organize the conversations of high-level managers to compare and integrate the motivations of corporate M&As to conduct confirmatory analysis. Using case studies and cross-border M&A related secondary data from 2014 to 2021 and supplemented by senior executives’ conversations, 1,836 articles were collected as analysis units. The research results show that Chinese companies’ cross-border M&A’s main corporate strategic motives have four key elements: accelerated expansion, integration of resources, brand integration, rapid entry into the international market, and obstacles to the construction of new entrants. The research results also show that integrating resources and brand execution strategies after M&As correlates to their success or failure. The purpose of the research was first to discuss brand M&As and corporate strategies in the Taiwanese context. Secondly, it discusses the issue of the use of resources by the acquired party in specific to emerging trends in consumer resistance to innovation and acceptance of technological innovativeness in the sports industry brands. Third, it analyzes the effectiveness of brand strategy integration and implementation. Finally, it provides a strategic reference for brand M&As in the industry.
The Effect of Self-Discrepancy on Online Behavior: A Literature Review
Authors: Hu Chuan , Cao Rui , Huang Jiao , Wei Ying
Published: April 2022

People will experience self-discrepancy when they perceive their actual self is distanced from their ideal self and ought self. The perceived self-discrepancy has a significant impact on people’s online behavior. The goal of this paper is to provide an overview of the research on self-discrepancy in the Internet era through a literature review. A total of 37 papers were included in this review. First, this study presents the basic characteristic of the reviewed articles (such as research contexts, samples, and data collection methods). Second, it summarized how the Internet affected people’s self-discrepancy. Third, this review identified how self-discrepancy was associated with different kinds of online behavior (such as impression management, knowledge contribution, and gaming addiction). Finally, this study outlined several possible directions for future research.
Fear of Progression, Anxiety, and Depression in Patients With Advanced Melanoma in the COVID-19 and Post-COVID-19 Era
Authors: Wang Xiaowen , Li Min , Shi Qiong , Ji Hongchen , Kong Shengnan , Zhu Lei , Zhang Hong-Mei
Published: April 2022

Background

The novel coronavirus disease 2019 (COVID-19) pandemic causes great disruption to cancer care services, which might bring about psychological problems and further lower both physical and mental life quality in cancer patients. Until now, very few studies focused on the psychological distress of patients with advanced melanoma before or during the epidemic. This study aimed to elucidate the fear of progression (FoP), anxiety, depression, and related independent predictors in patients with advanced melanoma during the COVID-19 outbreak.

Methods

Two hundred and seventy-three patients with unresectable stage III or metastatic melanoma were recruited from February 2020 to November 2021, and completed the Fear of Progression Questionnaire-Short Form (FoP-Q-SF), State Trait Anxiety Inventory (STAI-6), and Patient Health Questionnaire (PHQ-9).

Results

One hundred and seventy-four (64.7%) patients experienced heighted FoP (FoP-Q-SF: 39.9 ± 11.0), 198 (72.5%) patients reported elevated anxiety (STAI-6: 13.1 ± 3.0), and 62 (22.7%) patients had increased depression (PHQ-9: 6.4 ± 6.1). In multivariate analysis, illness duration (OR = 0.987 for FoP; OR = 0.984 for depression), cancer stage (OR = 14.394 for anxiety) and disease progression (OR = 1.960 for FoP; OR = 23.235 for anxiety; OR = 1.930 for depression) were independent predictors for FoP, anxiety or depression. Additionally, the high levels of FoP, anxiety and depression were significantly positive correlated with each other (r = 0.466 for FoP and anxiety; r = 0.382 for FoP and depression; r = 0.309 for anxiety and depression).

Conclusion

Our study indicates that FoP, anxiety and depression are persisting among patients with advanced melanoma in the COVID-19 and post-COVID-19 era. Effective psycho-oncological interventions are needed for melanoma patients with psychological distress during the ongoing COVID-19 pandemic.
Gendered Analysis of Cyberbullying Victimization and Its Associations with Suicidality: Findings from the 2019 Youth Risk Behavior Survey
Authors: Levine Rebecca S. , Bintliff Amy Vatne , Raj Anita
Published: April 2022

Cyberbullying victimization (CV), a widespread experience in adolescence, is associated with increased depression and suicidality. However, few studies have taken a gender approach when investigating the association between CV and suicidality, despite research that indicates disparate experiences by gender for both CV and mental health. We conducted a secondary data analysis of the 2019 Youth Risk Behavior Survey (N = 10,309; 50.1% girls), a cross-sectional survey drawn from a representative sample of US high school students. We found that CV remained significantly associated with suicidality after controlling for emotional and behavioral risk factors, for both boys and girls. CV increased the odds of suicidality directly and indirectly by increasing risk for depression, for both boys and girls. Boys contending with both CV and sexual violence were particularly vulnerable to suicidality, and binge drinking was positively associated with CV for girls but negatively associated with CV for boys. Findings confirmed that CV is a pervasive issue among U.S. adolescents. A gendered approach is necessary in order to understand and address the effects of CV.
Feeding Date-Palm Leaves Ensiled with Fibrolytic Enzymes or Multi-Species Probiotics to Farafra Ewes: Intake, Digestibility, Ruminal Fermentation, Blood Chemistry, Milk Production and Milk Fatty Acid Profile
Authors: Kholif Ahmed E. , Hamdon Hatem A. , Gouda Gouda A. , Kassab Ayman Y. , Morsy Tarek A. , Patra Amlan K.
Published: April 2022

The present experiment evaluated the feeding of date palm leaves (DPL) ensiled with fibrolytic enzymes (ENZ) or multi-species probiotics (MSP) on nutrient utilization and lactational performance of ewes. Fifty multiparous lactating Farafra ewes were used in a completely randomized design for 90 d. The treatments consisted of the control diet with a concentrate feed mixture and date palm leaves (at 60:40, DM basis) ensiled without additive (control) or DPL ensiled with ENZ or MSP replacing control DPL at 50 or 100%. Both ENZ and MSP increased (p < 0.01) DPL and total intakes, digestibility of all nutrients, concentrations of ammonia, total volatile fatty acids, acetate and propionate in the rumen. Increased milk production, concentrations of fat, lactose and energy in milk, and feed efficiency were observed with MSP and ENZ compared to the control treatment. Moreover, ENZ and MSP increased (p < 0.05) the concentrations of total n3, n6 fatty acids, polyunsaturated fatty acids and conjugated linoleic acids and decreased (p < 0.001) the atherogenicity. The differences between ENZ and MSP and between the low and high replacement levels were minor for all measured parameters. Ensiling of DPL with MSP or fibrolytic enzymes is recommended to improve feed efficiency and improve lactational performance of ewes.
Fish Oil Replacement by a Blend of Vegetable Oils in Diets for Juvenile Tench (Tinca tinca Linnaeus, 1758): Effects on Growth Performance and Whole-Body Composition
Authors: Sáez-Royuela María , García Teresa , Carral José M. , Celada Jesús D.
Published: April 2022

Among freshwater species, tench (Tinca tinca Linnaeus, 1758) is considered as a promising species for the diversification of aquaculture, but the intensification of techniques is necessary to promote and consolidate its culture. Adequate feeding in early growth phases is essential to face further grow-out. Fish oil (FO) is the main source of lipids in fish diets, but its production is unsustainable, and thus, alternative oils should be considered. A 90-day experiment was performed to evaluate the effects of partial and total replacement of cod liver oil (FO) by a blend of vegetable oils (VO) in juvenile tench. Six isonitrogenous and isolipidic diets with different levels of a VO blend containing 30% linseed oil, 20% corn oil, and 50% olive oil were tested: 0% (control), 20%VO, 40%VO, 60%VO, 80%VO, and 100%VO. With all diets, survival was 100%, and there were not differences in growth performance (total length (TL); weight (W); specific growth rate (SGR); feed conversion ratio (FCR); and biomass gain (BG)). Compared to the control group, whole-body lipid content decreased significantly in the 100%VO group. No differences in total saturated (SFA), monounsaturated (MUFA), and polyunsaturated (PUFA) fatty acids were found in the whole-body. The content of linolenic acid (ALA) in the whole-body increased as FO substitution did, being significantly higher with respect to control diet from 40% FO replacement diets. Nutritional indices, such as ΣPUFA/ΣSFA and Σn-6/Σn-3 ratios, tended to increase with increasing VO content, whereas the EPA + DHA showed an opposite trend. A total replacement of FO by the blend of VO did not affect the growth performance and fatty acid profile of juvenile tench. Further research on the effects of VO diet on nutritional quality in tench reared to commercial size should be performed.
Modeling Wicking Fabric Inhibition Effect on Frost Heave
Authors: Wu Yuwei , Ishikawa Tatsuya , Maruyama Kimio , Ueno Chigusa , Yasuoka Tomohisa , Okuda Sho
Published: April 2022

The deterioration of roads in cold regions can result in unsafe driving conditions and high maintenance costs. Frost heaving is regarded as one of the main reasons for road degradation. Generally, frost heave is caused by water migrating from the unfrozen zone to the freezing front, where it is then transformed into an ice lens. Frost heave can be reduced by removing frost-susceptible soil, raising the temperature, or removing water from the soil. Among these methods, the most economical and practical approach is to reduce the water content. Recently, an innovative geotextile known as wicking fabric (WF) has been used to drain water from unsaturated conditions and minimize frost heaving. The objective of this study was to evaluate the inhibition effects of WF on frost heave under different experimental conditions in the freezing process. In this study, a thermo-hydro-mechanical (THM) coupled numerical model is proposed to simulate the freezing process of subgrade soil with WF. The evaporation model is used to simply describe the water absorption characteristics of WF. The numerical model was validated by comparing the simulation results with the experimental results of the wicking fabric model (WWF) and the non-wicking fabric model (NWF). Additionally, parametric analysis was conducted to examine the effectiveness of WF in reducing frost heave under various experimental conditions. As a result, the freezing process of soil installed with WF was accurately simulated by the proposed model. WF showed inhibition effects on frost heave under various experimental conditions. The results indicate the following: (1) Compared to Touryo soil (a high frost-susceptible clay-sand soil), WF inhibited frost heave more effectively in Tomakomai soil (a medium frost-susceptible lean clay), while the inhibition effect of WF in Fujinomori soil (a medium frost-susceptible lean clay) was limited. (2) WF has a more significant frost heave inhibition effect at a slower cooling rate in the freezing process. (3) The further the WF is installed from the groundwater level (GWL), the greater its impact on inhibiting frost heave.
Changes in Weather-Related Fatalities in the Czech Republic during the 1961–2020 Period
Authors: Brázdil Rudolf , Chromá Kateřina , Zahradníček Pavel , Dobrovolný Petr , Dolák Lukáš , Řehoř Jan , Řezníčková Ladislava
Published: April 2022

Fatalities associated with severe weather, collected from newspapers and other documentary sources, were used to create a corresponding database for the 1961–2020 period for the Czech Republic. Fatalities attributed to floods, windstorms, convective storms, snow and glaze ice, frost, fog, and other severe weather, on the one hand, and vehicle accident fatalities connected with rain, snow, glaze ice, fog, and inclement weather, on the other, were analysed separately for two standard periods, 1961–1990 and 1991–2020. The number of weather-related fatalities between these two periods increased in the flood, windstorm, and especially frost categories, and decreased for the convective storm and fog categories. For snow and glaze ice they were the same. Despite significant differences in both 30-year periods, the highest proportions of fatalities corresponded to the winter months, and in individual fatality characteristics to males, adults, direct deaths, deaths by freezing or hypothermia, and to hazardous behaviour. A statistically significant (p < 0.05) Spearman rank correlation between fatalities and climate variables was only found in the 1991–2020 period for snow/glaze ice-related fatalities, with the number of days with snow cover depth and frost-related fatalities having days with daily minimum temperatures below −5 °C or −10 °C. Despite the highest proportions of the rain and wet road categories being in the number of vehicle accident fatalities, a statistically significant correlation was only found for the category of snow-related fatalities in the number of days with snowfall. The results and conclusions of this study have to be evaluated in the broader context of climatological, political, economic, and societal changes within the country, and have the potential to be used in risk management.
Advances in Recapitulating Alzheimer’s Disease Phenotypes Using Human Induced Pluripotent Stem Cell-Based In Vitro Models
Authors: Hasan Md Fayad , Trushina Eugenia
Published: April 2022

Alzheimer’s disease (AD) is an incurable neurodegenerative disorder and the leading cause of death among older individuals. Available treatment strategies only temporarily mitigate symptoms without modifying disease progression. Recent studies revealed the multifaceted neurobiology of AD and shifted the target of drug development. Established animal models of AD are mostly tailored to yield a subset of disease phenotypes, which do not recapitulate the complexity of sporadic late-onset AD, the most common form of the disease. The use of human induced pluripotent stem cells (HiPSCs) offers unique opportunities to fill these gaps. Emerging technology allows the development of disease models that recapitulate a brain-like microenvironment using patient-derived cells. These models retain the individual’s unraveled genetic background, yielding clinically relevant disease phenotypes and enabling cost-effective, high-throughput studies for drug discovery. Here, we review the development of various HiPSC-based models to study AD mechanisms and their application in drug discovery.