bluuebunny/crossref_metadata_2025 huggingface dataset:
Declined Organs for Liver Transplantation: A Right Decision or a Missed Opportunity for Patients with Hepatocellular Carcinoma?
Authors: Lozanovski Vladimir J. , Adigozalov Said , Khajeh Elias , Ghamarnejad Omid , Aminizadeh Ehsan , Schleicher Christina , Hackert Thilo , Müller-Stich Beat Peter , Merle Uta , Picardi Susanne , Lund Frederike , Chang De-Hua , Mieth Markus , Fonouni Hamidreza , Golriz Mohammad , Mehrabi Arianeb
Published: February 2023

Background: Liver transplantation is the only promising treatment for end-stage liver disease and patients with hepatocellular carcinoma. However, too many organs are rejected for transplantation. Methods: We analyzed the factors involved in organ allocation in our transplant center and reviewed all livers that were declined for transplantation. Reasons for declining organs for transplantation were categorized as major extended donor criteria (maEDC), size mismatch and vascular problems, medical reasons and risk of disease transmission, and other reasons. The fate of the declined organs was analyzed. Results: 1086 declined organs were offered 1200 times. A total of 31% of the livers were declined because of maEDC, 35.5% because of size mismatch and vascular problems, 15.8% because of medical reasons and risk of disease transmission, and 20.7% because of other reasons. A total of 40% of the declined organs were allocated and transplanted. A total of 50% of the organs were completely discarded, and significantly more of these grafts had maEDC than grafts that were eventually allocated (37.5% vs. 17.7%, p < 0.001). Conclusion: Most organs were declined because of poor organ quality. Donor-recipient matching at time of allocation and organ preservation must be improved by allocating maEDC grafts using individualized algorithms that avoid high-risk donor-recipient combinations and unnecessary organ declination.
Rational Targets of Therapy in Extranodal NK/T-Cell Lymphoma
Authors: Major Ajay , Porcu Pierluigi , Haverkos Bradley M.
Published: February 2023

Extranodal NK/T-cell lymphoma (ENKTL) is an aggressive extranodal non-Hodgkin lymphoma (NHL) with poor outcomes, particularly in advanced-stage and relapsed/refractory disease. Emerging research on molecular drivers of ENKTL lymphomagenesis by next-generation and whole genome sequencing has revealed diverse genomic mutations in multiple signaling pathways, with the identification of multiple putative targets for novel therapeutic agents. In this review, we summarize the biological underpinnings of newly-understood therapeutic targets in ENKTL with a focus on translational implications, including epigenetic and histone regulatory aberrations, activation of cell proliferation signaling pathways, suppression of apoptosis and tumor suppressor genes, changes in the tumor microenvironment, and EBV-mediated oncogenesis. In addition, we highlight prognostic and predictive biomarkers which may enable a personalized medicine approach toward ENKTL therapy.
Predicting Patient-Specific Tumor Dynamics: How Many Measurements Are Necessary?
Authors: Harshe Isha , Enderling Heiko , Brady-Nicholls Renee
Published: February 2023

Acquiring sufficient data is imperative to accurately predict tumor growth dynamics and effectively treat patients. The aim of this study was to investigate the number of volume measurements necessary to predict breast tumor growth dynamics using the logistic growth model. The model was calibrated to tumor volume data from 18 untreated breast cancer patients using a varying number of measurements interpolated at clinically relevant timepoints with different levels of noise (0–20%). Error-to-model parameters and the data were compared to determine the sufficient number of measurements needed to accurately determine growth dynamics. We found that without noise, three tumor volume measurements are necessary and sufficient to estimate patient-specific model parameters. More measurements were required as the level of noise increased. Estimating the tumor growth dynamics was shown to depend on the tumor growth rate, clinical noise level, and acceptable error of the to-be-determined parameters. Understanding the relationship between these factors provides a metric by which clinicians can determine when sufficient data have been collected to confidently predict patient-specific tumor growth dynamics and recommend appropriate treatment options.
USP15 Represses Hepatocellular Carcinoma Progression by Regulation of Pathways of Cell Proliferation and Cell Migration: A System Biology Analysis
Authors: Ren Yiyue , Song Zhen , Rieser Jens , Ackermann Jörg , Koch Ina , Lv Xingyu , Ji Tong , Cai Xiujun
Published: February 2023

Background: Hepatocellular carcinoma (HCC) leads to 600,000 people’s deaths every year. The protein ubiquitin carboxyl-terminal hydrolase 15 (USP15) is a ubiquitin-specific protease. The role of USP15 in HCC is still unclear. Method: We studied the function of USP15 in HCC from the viewpoint of systems biology and investigated possible implications using experimental methods, such as real-time polymerase chain reaction (qPCR), Western blotting, clustered regularly interspaced short palindromic repeats (CRISPR), and next-generation sequencing (NGS). We investigated tissues samples of 102 patients who underwent liver resection between January 2006 and December 2010 at the Sir Run Run Shaw Hospital (SRRSH). Tissue samples were immunochemically stained; a trained pathologist then scored the tissue by visual inspection, and we compared the survival data of two groups of patients by means of Kaplan–Meier curves. We applied assays for cell migration, cell growth, and wound healing. We studied tumor formation in a mouse model. Results: HCC patients (n = 26) with high expression of USP15 had a higher survival rate than patients (n = 76) with low expression. We confirmed a suppressive role of USP15 in HCC using in vitro and in vivo tests. Based on publicly available data, we constructed a PPI network in which 143 genes were related to USP15 (HCC genes). We combined the 143 HCC genes with results of an experimental investigation to identify 225 pathways that may be related simultaneously to USP15 and HCC (tumor pathways). We found the 225 pathways enriched in the functional groups of cell proliferation and cell migration. The 225 pathways determined six clusters of pathways in which terms such as signal transduction, cell cycle, gene expression, and DNA repair related the expression of USP15 to tumorigenesis. Conclusion: USP15 may suppress tumorigenesis of HCC by regulating pathway clusters of signal transduction for gene expression, cell cycle, and DNA repair. For the first time, the tumorigenesis of HCC is studied from the viewpoint of the pathway cluster.
Prostate Cancer Morphologies: Cribriform Pattern and Intraductal Carcinoma Relations to Adverse Pathological and Clinical Outcomes—Systematic Review and Meta-Analysis
Authors: Osiecki Rafał , Kozikowski Mieszko , Sarecka-Hujar Beata , Pyzlak Michał , Dobruch Jakub
Published: February 2023

The present study aimed to assess the association between the cribriform pattern (CP)/intraductal carcinoma (IDC) and the adverse pathological and clinical outcomes in the radical prostatectomy (RP) cohort. A systematic search was performed according to the Preferred Reporting Items for Systematic Review and Meta-Analysis statement (PRISMA). The protocol from this review was registered on the PROSPERO platform. We searched PubMed®, the Cochrane Library and EM-BASE® up to the 30th of April 2022. The outcomes of interest were the extraprostatic extension (EPE), seminal vesicle invasion (SVI), lymph node metastasis (LNS met), risk of biochemical recurrence (BCR), distant metastasis (MET) and disease-specific death (DSD). As a result, we identified 16 studies with 164 296 patients. A total of 13 studies containing 3254 RP patients were eligible for the meta-analysis. The CP/IDC was associated with adverse outcomes, including EPE (pooled OR = 2.55, 95%CI 1.23–5.26), SVI (pooled OR = 4.27, 95%CI 1.90–9.64), LNs met (pooled OR = 6.47, 95%CI 3.76–11.14), BCR (pooled OR = 5.09, 95%CI 2.23–11.62) and MET/DSD (pooled OR = 9.84, 95%CI 2.75–35.20, p < 0.001). In conclusion, the CP/IDC belong to highly malignant prostate cancer patterns which have a negative impact on both the pathological and clinical outcomes. The presence of the CP/IDC should be included in the surgical planning and postoperative treatment guidance.
Radiomic Features Associated with Lymphoma Development in the Parotid Glands of Patients with Primary Sjögren’s Syndrome
Authors: Muntean Delia Doris , Lenghel Lavinia Manuela , Ștefan Paul Andrei , Fodor Daniela , Bădărînză Maria , Csutak Csaba , Dudea Sorin Marian , Rusu Georgeta Mihaela
Published: February 2023

Non-Hodgkin Lymphoma (NHL) represents a severe complication and the main cause of morbidity in patients with primary Sjögren’s syndrome (pSS). This study aimed to assess the role of textural analysis (TA) in revealing lymphoma-associated imaging parameters in the parotid gland (PG) parenchyma of patients with pSS. This retrospective study included a total of 36 patients (54.93 ± 13.34 years old; 91.6% females) diagnosed with pSS according to the American College of Rheumatology and the European League Against Rheumatism criteria (24 subjects with pSS and no lymphomatous proliferation; 12 subjects with pSS and NHL development in the PG, confirmed by the histopathological analysis). All subjects underwent MR scanning between January 2018 and October 2022. The coronal STIR PROPELLER sequence was employed to segment PG and perform TA using the MaZda5 software. A total of 65 PGs underwent segmentation and texture feature extraction (48 PGs were included in the pSS control group, and 17 PGs were included in the pSS NHL group). Following parameter reduction techniques, univariate analysis, multivariate regression, and receiver operating characteristics (ROC) analysis, the following TA parameters proved to be independently associated with NHL development in pSS: CH4S6_Sum_Variance and CV4S6_Inverse_Difference_Moment, with an area under ROC of 0.800 and 0.875, respectively. The radiomic model (resulting by combining the two previously independent TA features), presented 94.12% sensitivity and 85.42% specificity in differentiating between the two studied groups, reaching the highest area under ROC of 0.931 for the chosen cutoff value of 1.556. This study suggests the potential role of radiomics in revealing new imaging biomarkers that might serve as useful predictors for lymphoma development in patients with pSS. Further research on multicentric cohorts is warranted to confirm the obtained results and the added benefit of TA in risk stratification for patients with pSS.
Unlocking the Resistance to Anti-HER2 Treatments in Breast Cancer: The Issue of HER2 Spatial Distribution
Authors: Giugliano Federica , Carnevale Schianca Ambra , Corti Chiara , Ivanova Mariia , Bianco Nadia , Dellapasqua Silvia , Criscitiello Carmen , Fusco Nicola , Curigliano Giuseppe , Munzone Elisabetta
Published: February 2023

Approximately 15% of breast cancers are classified as HER2-positive, with an amplification of the ERBB2 gene and/or an overexpression of the HER2 protein. Up to 30% of HER2-positive breast cancers shows heterogeneity in HER2 expression and different patterns of spatial distribution, i.e., the variability in the distribution and expression of the HER2 protein within a single tumour. Spatial heterogeneity may potentially affect treatment, response, assessment of HER2 status and consequently, may impact on the best treatment strategy. Understanding this feature can help clinicians to predict response to HER2-targeted therapies and patient outcomes, and to fine tune treatment decisions. This review summarizes the available evidence on HER2 heterogeneity and spatial distribution and how this may affect current available treatment choices, exploring possible opportunities for overcoming this issue, such as novel pharmacological agents, belonging to the group of antibody–drug conjugates.
The Catalytic Performance of Nanorod Nickel Catalyst in the Hydrolysis of Lithium Borohydride and Dimethylamine Borane
Authors: Akkus Meryem Sena
Published: February 2023

In the current global energy crisis, the value of hydrogen has become better appreciated. Metal borohydrides attract a lot of attention from researchers because they are rich in hydrogen. In this study, glass microscope slides were coated with nickel as nanorods for use as a catalyst by the magnetron sputtering method, and then catalytic hydrolysis reactions of dimethylamine borane and lithium borohydride were carried out to produce hydrogen. Parameters such as temperature, the amount of catalyst, lithium borohydride, or dimethylamine borane concentration were varied and their effects on the catalytic performances of the catalyst were examined. Moreover, the catalyst was characterized by field emission scanning electron microscopy and X-ray diffraction, and hydrolysis products were analyzed through field emission scanning electron microscopy with energy dispersive spectroscopy analyses. Reaction kinetic parameters were also determined. The activation energy values of dimethylamine borane and lithium borohydride were determined to be 40.0 kJ mol−1 and 63.74 kJ mol−1, respectively. Activation enthalpy values were also calculated as 37.34 kJ mol−1 and 62.45 kJ mol−1 for dimethylamine borane and lithium borohydride, respectively. Initial hydrogen production rates under different conditions were also investigated in the study. For both hydrolysis systems, the fastest hydrogen production rates were calculated as 109 mL gNi−1 min−1 and 103 mL gNi−1 min−1 for dimethylamine borane and lithium borohydride, respectively, in the experiment performed at 60 °C at 0.2 M substrate concentration and with 1.3 g of catalyst. These hydrolysis systems using this catalyst are good candidates for systems that need hydrogen.
Modelling Cancer Metastasis in Drosophila melanogaster
Authors: Sharpe Joanne L. , Morgan Jason , Nisbet Nicholas , Campbell Kyra , Casali Andreu
Published: February 2023

Cancer metastasis, the process by which tumour cells spread throughout the body and form secondary tumours at distant sites, is the leading cause of cancer-related deaths. The metastatic cascade is a highly complex process encompassing initial dissemination from the primary tumour, travel through the blood stream or lymphatic system, and the colonisation of distant organs. However, the factors enabling cells to survive this stressful process and adapt to new microenvironments are not fully characterised. Drosophila have proven a powerful system in which to study this process, despite important caveats such as their open circulatory system and lack of adaptive immune system. Historically, larvae have been used to model cancer due to the presence of pools of proliferating cells in which tumours can be induced, and transplanting these larval tumours into adult hosts has enabled tumour growth to be monitored over longer periods. More recently, thanks largely to the discovery that there are stem cells in the adult midgut, adult models have been developed. We focus this review on the development of different Drosophila models of metastasis and how they have contributed to our understanding of important factors determining metastatic potential, including signalling pathways, the immune system and the microenvironment.
A Beginner’s Guide to Cell Culture: Practical Advice for Preventing Needless Problems
Authors: Weiskirchen Sabine , Schröder Sarah K. , Buhl Eva Miriam , Weiskirchen Ralf
Published: February 2023

The cultivation of cells in a favorable artificial environment has become a versatile tool in cellular and molecular biology. Cultured primary cells and continuous cell lines are indispensable in investigations of basic, biomedical, and translation research. However, despite their important role, cell lines are frequently misidentified or contaminated by other cells, bacteria, fungi, yeast, viruses, or chemicals. In addition, handling and manipulating of cells is associated with specific biological and chemical hazards requiring special safeguards such as biosafety cabinets, enclosed containers, and other specialized protective equipment to minimize the risk of exposure to hazardous materials and to guarantee aseptic work conditions. This review provides a brief introduction about the most common problems encountered in cell culture laboratories and some guidelines on preventing or tackling respective problems.
RNA-Seq Analysis of Extradomain A and Extradomain B Fibronectin as Extracellular Matrix Markers for Cancer
Authors: Hall Ryan C. , Vaidya Amita M. , Schiemann William P. , Pan Quintin , Lu Zheng-Rong
Published: February 2023

Alternatively spliced forms of fibronectin, called oncofetal fibronectin, are aberrantly expressed in cancer, with little to no expression in normal tissue, making them attractive biomarkers to exploit for tumor-targeted therapeutics and diagnostics. While prior studies have explored oncofetal fibronectin expression in limited cancer types and limited sample sizes, no studies have performed a large-scale pan-cancer analysis in the context of clinical diagnostics and prognostics to posit the utility of these biomarkers across multiple cancer types. In this study, RNA-Seq data sourced from the UCSC Toil Recompute project were extracted and analyzed to determine the correlation between the expression of oncofetal fibronectin, including extradomain A and extradomain B fibronectin, and patient diagnosis and prognosis. We determined that oncofetal fibronectin is significantly overexpressed in most cancer types relative to corresponding normal tissues. In addition, strong correlations exist between increasing oncofetal fibronectin expression levels and tumor stage, lymph node activity, and histological grade at the time of diagnosis. Furthermore, oncofetal fibronectin expression is shown to be significantly associated with overall patient survival within a 10-year window. Thus, the results presented in this study suggest oncofetal fibronectin as a commonly upregulated biomarker in cancer with the potential to be used for tumor-selective diagnosis and treatment applications.
Hydroquinone, an Environmental Pollutant, Affects Cartilage Homeostasis through the Activation of the Aryl Hydrocarbon Receptor Pathway
Authors: Heluany Cintia Scucuglia , De Palma Anna , Day Nicholas James , Farsky Sandra Helena Poliselli , Nalesso Giovanna
Published: February 2023

Exposure to environmental pollutants has a proven detrimental impact on different aspects of human health. Increasing evidence has linked pollution to the degeneration of tissues in the joints, although through vastly uncharacterised mechanisms. We have previously shown that exposure to hydroquinone (HQ), a benzene metabolite that can be found in motor fuels and cigarette smoke, exacerbates synovial hypertrophy and oxidative stress in the synovium. To further understand the impact of the pollutant on joint health, here we investigated the effect of HQ on the articular cartilage. HQ exposure aggravated cartilage damage in rats in which inflammatory arthritis was induced by injection of Collagen type II. Cell viability, cell phenotypic changes and oxidative stress were quantified in primary bovine articular chondrocytes exposed to HQ in the presence or absence of IL-1β. HQ stimulation downregulated phenotypic markers genes SOX-9 and Col2a1, whereas it upregulated the expression of the catabolic enzymes MMP-3 and ADAMTS5 at the mRNA level. HQ also reduced proteoglycan content and promoted oxidative stress alone and in synergy with IL-1β. Finally, we showed that HQ-degenerative effects were mediated by the activation of the Aryl Hydrocarbon Receptor. Together, our findings describe the harmful effects of HQ on articular cartilage health, providing novel evidence surrounding the toxic mechanisms of environmental pollutants underlying the onset of articular diseases.
Role of SIRT3 in Microgravity Response: A New Player in Muscle Tissue Recovery
Authors: Aventaggiato Michele , Barreca Federica , Vitiello Laura , Vespa Simone , Valente Sergio , Rotili Dante , Mai Antonello , Lotti Lavinia Vittoria , Sansone Luigi , Russo Matteo A. , Bizzarri Mariano , Ferretti Elisabetta , Tafani Marco
Published: February 2023

Life on Earth has evolved in the presence of a gravity constraint. Any change in the value of such a constraint has important physiological effects. Gravity reduction (microgravity) alters the performance of muscle, bone and, immune systems among others. Therefore, countermeasures to limit such deleterious effects of microgravity are needed considering future Lunar and Martian missions. Our study aims to demonstrate that the activation of mitochondrial Sirtuin 3 (SIRT3) can be exploited to reduce muscle damage and to maintain muscle differentiation following microgravity exposure. To this effect, we used a RCCS machine to simulate microgravity on ground on a muscle and cardiac cell line. During microgravity, cells were treated with a newly synthesized SIRT3 activator, called MC2791 and vitality, differentiation, ROS and, autophagy/mitophagy were measured. Our results indicate that SIRT3 activation reduces microgravity-induced cell death while maintaining the expression of muscle cell differentiation markers. In conclusion, our study demonstrates that SIRT3 activation could represent a targeted molecular strategy to reduce muscle tissue damage caused by microgravity.
Tandem Multimerization Can Enhance the Structural Homogeneity and Antifungal Activity of the Silkworm Protease Inhibitor BmSPI39
Authors: Li Youshan , Wang Yuan , Zhu Rui , Yang Xi , Wei Meng , Zhang Zhaofeng , Chen Changqing , Zhao Ping
Published: February 2023

Previous studies have shown that BmSPI39, a serine protease inhibitor of silkworm, can inhibit virulence-related proteases and the conidial germination of insect pathogenic fungi, thereby enhancing the antifungal capacity of Bombyx mori. The recombinant BmSPI39 expressed in Escherichia coli has poor structural homogeneity and is prone to spontaneous multimerization, which greatly limits its development and application. To date, the effect of multimerization on the inhibitory activity and antifungal ability of BmSPI39 remains unknown. It is urgent to explore whether a BmSPI39 tandem multimer with better structural homogeneity, higher activity and a stronger antifungal ability can be obtained by protein engineering. In this study, the expression vectors of BmSPI39 homotype tandem multimers were constructed using the isocaudomer method, and the recombinant proteins of tandem multimers were obtained by prokaryotic expression. The effects of BmSPI39 multimerization on its inhibitory activity and antifungal ability were investigated by protease inhibition and fungal growth inhibition experiments. In-gel activity staining and protease inhibition assays showed that tandem multimerization could not only greatly improve the structural homogeneity of the BmSPI39 protein, but also significantly increase its inhibitory activity against subtilisin and proteinase K. The results of conidial germination assays showed that tandem multimerization could effectively enhance the inhibitory ability of BmSPI39 on the conidial germination of Beauveria bassiana. A fungal growth inhibition assay showed that BmSPI39 tandem multimers had certain inhibitory effects on both Saccharomyces cerevisiae and Candida albicans. The inhibitory ability of BmSPI39 against these the above two fungi could be enhanced by tandem multimerization. In conclusion, this study successfully achieved the soluble expression of tandem multimers of the silkworm protease inhibitor BmSPI39 in E. coli and confirmed that tandem multimerization can improve the structural homogeneity and antifungal ability of BmSPI39. This study will not only help to deepen our understanding of the action mechanism of BmSPI39, but also provide an important theoretical basis and new strategy for cultivating antifungal transgenic silkworms. It will also promote its exogenous production and development and application in the medical field.
Intrahippocampal Inoculation of Aβ1–42 Peptide in Rat as a Model of Alzheimer’s Disease Identified MicroRNA-146a-5p as Blood Marker with Anti-Inflammatory Function in Astrocyte Cells
Authors: Aquino Ruth , de Concini Vidian , Dhenain Marc , Lam Suzanne , Gosset David , Baquedano Laura , Forero Manuel G. , Menuet Arnaud , Baril Patrick , Pichon Chantal
Published: February 2023

Circulating microRNAs (miRNAs) have aroused a lot of interest as reliable blood diagnostic biomarkers of Alzheimer’s disease (AD). Here, we investigated the panel of expressed blood miRNAs in response to aggregated Aβ1–42 peptides infused in the hippocampus of adult rats to mimic events of the early onset of non-familial AD disorder. Aβ1–42 peptides in the hippocampus led to cognitive impairments associated with an astrogliosis and downregulation of circulating miRNA-146a-5p, -29a-3p, -29c-3p, -125b-5p, and-191-5p. We established the kinetics of expression of selected miRNAs and found differences with those detected in the APPswe/PS1dE9 transgenic mouse model. Of note, miRNA-146a-5p was exclusively dysregulated in the Aβ-induced AD model. The treatment of primary astrocytes with Aβ1–42 peptides led to miRNA-146a-5p upregulation though the activation of the NF-κB signaling pathway, which in turn downregulated IRAK-1 but not TRAF-6 expression. As a consequence, no induction of IL-1β, IL-6, or TNF-α was detected. Astrocytes treated with a miRNA-146-5p inhibitor rescued IRAK-1 and changed TRAF-6 steady-state levels that correlated with the induction of IL-6, IL-1β, and CXCL1 production, indicating that miRNA-146a-5p operates anti-inflammatory functions through a NF-κB pathway negative feedback loop. Overall, we report a panel of circulating miRNAs that correlated with Aβ1–42 peptides’ presence in the hippocampus and provide mechanistic insights into miRNA-146a-5p biological function in the development of the early stage of sporadic AD.
Optimization of Electrical Properties of Nanocrystallized Na3M2(PO4)2F3 NASICON-like Glasses (M = V, Ti, Fe)
Authors: Nowagiel Maciej , Hul Anton , Kazakevicius Edvardas , Kežionis Algimantas , Garbarczyk Jerzy E. , Pietrzak Tomasz K.
Published: February 2023

Recently, an interest in NASICON-type materials revived, as they are considered potential cathode materials in sodium–ion batteries used in large-scale energy storage. We applied a facile technique of thermal nanocrystallization of glassy analogs of these compounds to enhance their electrical parameters. Six nanomaterials of the Na3M2(PO4)2F3 (M = V, Ti, Fe) system were studied. Samples with nominal compositions of Na3V2(PO4)2F3, Na3Ti2(PO4)2F3, Na3Fe2(PO4)2F3, Na3TiV(PO4)2F3, Na3FeV(PO4)2F3 and Na3FeTi(PO4)2F3 have been synthesized as glasses using the melt-quenching method. X-ray diffraction measurements were conducted for as-synthesized samples and after heating at elevated temperatures to investigate the structure. Extensive impedance measurements allowed us to optimize the nanocrystallization process to enhance the electrical conductivity of cathode nanomaterials. Such a procedure resulted in samples with the conductivity at room temperature ranging from 1×10−9 up to 8×10−5 S/cm. We carried out in situ impedance spectroscopy measurements (in an ultra-high-frequency range up to 10 GHz) and compared them with thermal events observed in differential thermal analysis studies.
Designing Personas for E-Resources Users in the University Libraries
Authors: Rohmiyati Yuli , Tengku Wook Tengku Siti Meriam , Sahari Noraidah , Hanawi Siti Aishah , Qamar Faizan
Published: February 2023

Persona is a method to create a user profile by describing a fictitious user through user experience. This persona study needs to be carried out for the benefit of system design according to the users’ wishes because, so far, electronic resources (e-resources) are not widely used due to cognitive and affective factors such as limited subscription resources, limited user manuals, limited navigation features, and frequent errors when using electronic resources. This leaves the user feeling confused and stressed. The aim of this study is to obtain profiles of e-resource users in college libraries. The method used is an empathy map created with data from 32 users who answered questionnaires and participated in interviews. This study found four e-resource user personas in university libraries: lecturers, students, research assistants, and librarians. Users want a guide for using electronic resources that allows for chat and sharing, and which is fun and can be accessed from any device anytime and anywhere. The benefits of this study will be useful for designing e-resource systems according to users’ wishes.
Crystal Structure and Properties of Zinc Phosphate Layers on Aluminum and Steel Alloy Surfaces
Authors: Herbáth Beáta , Kovács Kristóf , Jakab Miklós , Makó Éva
Published: February 2023

Many studies have been carried out on the phosphating of steel and aluminum alloys used in automotive engineering, but characterization of the properties of the phosphate layers formed by the co-phosphating of these alloys in the presence of different base metals is still lacking. In this study, the crystal structure and properties of the phosphate conversion layers formed on the surface of the aluminum alloys important in vehicle manufacturing (cast and forged AlSi1MgMn, and AA6014 panel) and the CRS SAE 1008/1010 reference steel plate by co-deposition prior to painting were investigated. On a process line set up for the phosphating of typical iron and steel alloys, the phosphate coating was formed using nitrite and nitroguanidine accelerators under identical technological parameters. The microstructure of the formed phosphate layers was examined using scanning electron microscopy (SEM), its phase composition using X-ray diffraction (XRD), and its elemental composition using energy-dispersive X-ray analysis (EDX). The suggested main crystalline phase (Zn2.3(Ni0.1Mn0.6)(PO4)2·4H2O) in the surface phosphate layer of both aluminum alloys studied was similar to hopeite, whereas in the steel plate, a minor hopeite phase were identified in addition to the main crystalline phosphophyllite phase (~95%). It can be concluded that, during the combined phosphating treatments, the surfaces of different aluminum and steel alloys behaved similarly to the individual treatments and did not impede the coating reactions of the other metal. To obtain an adequate coating of aluminum and steel alloys, fluoride should always be present in the production line. Comparing the effects of accelerators, we found that the use of nitrite accelerator with the same amount of fluoride resulted in a higher coverage and better quality of the surface protective layer of the aluminum alloys. However, for the steel plate, there was no significant difference between the phosphate coatings prepared with the two different accelerators.
Plasma-Assisted Halide Vapor Phase Epitaxy for Low Temperature Growth of III-Nitrides
Authors: Pozina Galia , Hsu Chih-Wei , Abrikossova Natalia , Hemmingsson Carl
Published: February 2023

Developing growth techniques for the manufacture of wide band gap III-nitrides semiconductors is important for the further improvement of optoelectronic applications. A plasma-assisted halide phase vapor epitaxy (PA-HVPE) approach is demonstrated for the manufacture of undoped and In-doped GaN layers at ~600 °C. A dielectric barrier discharge (DBD) plasma source is utilized for the low-temperature activation of ammonia. The use of the plasma source at a growth temperature of ~600 °C increases the growth rate from ~1.2 to ~4–5 µm/h. Furthermore, the possibility for the growth of InGaN at ~600 °C has been studied. Precursors of GaCl and InCl/InCl3 are formed in situ in the reactor by flowing HCl gas over a melt of metallic Ga and In, respectively. The In concentration was low, in the order of a few percent, as the incorporation of In is reduced by plasma due to the activation of chlorine-containing species that etch the relatively poorly bonded In atoms. Nevertheless, the approach of using plasma for ammonia activation is a very promising approach to growing epitaxial III-nitrides at low temperatures.
Phenotypic Plasticity Drives the Successful Expansion of the Invasive Plant Pedicularis kansuensis in Bayanbulak, China
Authors: Li Wenchao , Huang Liju , Yang Lei , Liu Yanyan , Chen Huimei , Li Wenjun
Published: February 2023

To better understand the phenotypic plasticity of the highly invasive native weed, Pedicularis kansuensis, we investigated and compared phenotypes (morphology, biomass, and nutrient composition) at different levels of invasion (low: 0 < cover ≤ 30%; medium: 30% < cover ≤ 70%; and high: cover > 70%). With the increase in invasion level, the plasticity of inflorescence length, single-leaf thickness, and specific leaf area increased, while the plasticity of single-leaf area and crown width decreased. During the invasion process, we observed significant density-dependent effects, including changed morphological characteristics, increased total aboveground biomass, and decreased plant height, inflorescence length, root length, crown width, single-leaf area, structure biomass of structures (root, stem, inflorescence), and individual biomass (p < 0.05). During the reproductive period of P. kansuensis, the resource allocation (C, N, and P content, total biomass, biomass allocation) to inflorescence was significantly higher than to root and stem, while the elemental ratios (C:N, C:P, N:P) of inflorescences were significantly lower than those of roots and stems (p < 0.05). When the invasion level increased, the ratio of inflorescence C:N and biomass allocation to roots increased significantly; conversely, inflorescence N and biomass allocation to inflorescences and stems decreased significantly (p < 0.05). This led to a decrease in resource allocation to aboveground parts and more resources allocated to the roots, significantly increasing the root-to-shoot ratio (p < 0.05). Based on the phenotypic differences among different invasion levels, we suggest that P. kansuensis adapted to a competitive environment by regulating morphology, biomass, and nutrient allocation, thereby enhancing the potential of invasion and spread.
New Records of the Alien Chinese Ricefish (Oryzias sinensis) and Its Dispersal History across Eurasia
Authors: Makhrov Alexander A. , Artamonova Valentina S. , Sun Yue-Hua , Fang Yun , Pashkov Andrey N. , Reshetnikov Andrey N.
Published: February 2023

The diversity of biota in different parts of the planet has demonstrated dramatic changes within the last several decades due to the extinction of native taxa and the appearance of invasive taxa. The correct taxonomic identification of non-native species is important for understanding their dispersal abilities, especially when potential invaders may be of closely related species. Information on the species identity of ricefish (Oryzias spp.), which have formed self-sustainable populations in some parts of Eurasia, is contradictory. In this paper, we studied samples from non-native populations of Oryzias from several Eurasian regions. The results of our mtDNA COI partial sequence study confirm that the individuals we studied belong to the species Chinese ricefish, O. sinensis. Analyses of the literature and our own data suggest that all known alien populations of Oryzias in continental Eurasia belong to the same species, O. sinensis. A recent finding of O. sinensis in the Don delta suggests that one of the species’ secondary dispersal pathways could run from the Kuban region through the lower part of the Don basin to the Cis-Azov area.
A Devonian Fish Tale: A New Method of Body Length Estimation Suggests Much Smaller Sizes for Dunkleosteus terrelli (Placodermi: Arthrodira)
Authors: Engelman Russell K.
Published: February 2023

Dunkleosteus terrelli, an arthrodire placoderm, is one of the most widely recognized fossil vertebrates due to its large size and status as one of the earliest vertebrate apex predators. However, the exact size of this taxon is unclear due to its head and thoracic armor being the only elements of its body regularly preserved in the fossil record. Lengths of 5–10 m are commonly cited, but these estimates are not based on rigorous statistical analysis. Here, I estimate the body size of D. terrelli using a new metric, orbit-opercular length, and a large dataset of arthrodires and extant fishes (3169 observations, 972 species). Orbit-opercular length strongly correlates with total length in fishes (r2 = 0.947, PEcf = 17.55%), and accurately predicts body size in arthrodires known from complete remains. Applying this method to Dunkleosteus terrelli results in much smaller sizes than previous studies: 3.4 m for typical adults (CMNH 5768) with the largest known individuals (CMNH 5936) reaching ~4.1 m. Arthrodires have a short, deep, and cylindrical body plan, distinctly different from either actinopterygians or elasmobranchs. Large arthrodires (Dunkleosteus, Titanichthys) were much smaller than previously thought and vertebrates likely did not reach sizes of 5 m or greater until the Carboniferous.
Forest Disturbances Threatening Cypripedium calceolus Populations Can Improve Its Habitat Conditions
Authors: Křenová Zdenka , Lustyk Pavel , Kindlmann Pavel , Vosmíková Alžběta
Published: February 2023

Inappropriate forest management activities, above all clear-cutting, are recognized as the major threats to an iconic orchid species—the lady’s slipper orchid (Cypripedium calceolus), the flagship species of nature protection in Europe. Although clear-cutting in protected species’ localities is strongly regulated in many European countries, salvage logging is allowed in some circumstances (e.g., following windstorms, and insect outbreaks) and can threaten C. calceolus populations. In this paper, we review a database of recently registered populations of this species in the Czech Republic and study historical maps, to better understand the history of local habitat conditions and assess threats to C. calceolus populations by bark beetle outbreaks. We found that about one-third of the C. calceolus populations have suffered in coniferous or mixed tree plantations with a high proportion of spruce trees, which are forests with a high risk of bark beetle infestation. We review bark beetle management measures and distinguish management efforts in areas with known C. calceolus populations that result in no damage to the population and, at times, improve habitat conditions for the species. Thus, the bark beetle—usually understood as the enemy—can be transformed into a savior if smart management measures will replace the panic salvage logging.
Diagnostic Performance of Extrahepatic Protein Induced by Vitamin K Absence in the Hepatocellular Carcinoma: A Systematic Review and Meta-Analysis
Authors: Perne Mirela Georgiana , Sitar-Tăut Adela-Viviana , Alexescu Teodora Gabriela , Ciumărnean Lorena , Milaciu Mircea-Vasile , Coste Sorina-Cezara , Vlad Calin-Vasile , Cozma Angela , Sitar-Tăut Dan-Andrei , Orăşan Olga Hilda , Crăciun Alexandra
Published: February 2023

Background and Objectives: the early diagnosis of hepatocellular carcinoma (HCC) benefits from the use of alpha-fetoprotein (AFP) together with imaging diagnosis using abdominal ultrasonography, CT, and MRI, leading to improved early detection of HCC. A lot of progress has been made in the field, but some cases are missed or late diagnosed in advanced stages of the disease. Therefore, new tools (serum markers, imagistic technics) are continually being reconsidered. Serum alpha-fetoprotein (AFP), protein induced by vitamin K absence or antagonist II (PIVKA II) diagnostic accuracy for HCC (global and early disease) has been investigated (in a separate or cumulative way). The purpose of the present study was to determine the performance of PIVKA II compared to AFP. Materials and Methods: systematic research was conducted in PubMed, Web of Science, Embase, Medline and the Cochrane Central Register of Controlled Trials, taking into consideration articles published between 2018 and 2022. Results: a total number of 37 studies (5037 patients with HCC vs. 8199 patients—control group) have been included in the meta-analysis. PIVKA II presented a better diagnostic accuracy in HCC diagnostic vs. alpha-fetoprotein (global PIVKA II AUROC 0.851 vs. AFP AUROC 0.808, respectively, 0.790 vs. 0.740 in early HCC cases). The conclusion from a clinical point of view, concomitant use of PIVKA II and AFP can bring useful information, added to that brought by ultrasound examination.
Hypoplastic Left Heart Syndrome: About a Postnatal Death
Authors: Giugliano Pasquale , Ciamarra Paola , De Simone Mariavictoria , Feola Alessandro , Zangani Pierluca , Campobasso Carlo Pietro , Mansueto Gelsomina
Published: February 2023

Background: Hypoplastic left heart syndrome (HLHS) is a congenital heart disease that is associated with high mortality rates in the early neonatal period and during surgical treatments. This is mainly due to missed prenatal diagnosis, delayed diagnostic suspicion, and consequent unsuccessful therapeutic intervention. Case report: twenty-six hours after birth, a female newborn died of severe respiratory failure. No cardiac abnormalities and no genetic diseases had been evidenced or documented during intrauterine life. The case became of medico-legal concern for the assessment of alleged medical malpractice. Therefore, a forensic autopsy was performed. Results: the macroscopic study of the heart revealed the hypoplasia of the left cardiac cavities with the left ventricle (LV) reduced to a slot and a right ventricular cavity that simulated the presence of a single and unique ventricular chamber. The predominance of the left heart was evident. Conclusions: HLHS is a rare condition that is incompatible with life, with very high mortality from cardiorespiratory insufficiency that occurs soon after birth. The prompt diagnosis of HLHS during pregnancy is crucial in managing the disease with surgery.
Polyclonal Antibody Generation against PvTRAg for the Development of a Diagnostic Assay for Plasmodium vivax
Authors: Aggarwal Shalini , Selvaraj Selvamano , Subramanian Jayaprakash Nattamai , Vijayalakshmi Mookambeswaran Arunachalam , Patankar Swati , Srivastava Sanjeeva
Published: February 2023

The World Health Organization (WHO) has set forth a global call for eradicating malaria, caused majorly by the protozoan parasites Plasmodium falciparum and Plasmodium vivax. The lack of diagnostic biomarkers for P. vivax, especially those that differentiate the parasite from P. falciparum, significantly hinders P. vivax elimination. Here, we show that P. vivax tryptophan-rich antigen (PvTRAg) can be a diagnostic biomarker for diagnosing P. vivax in malaria patients. We report that polyclonal antibodies against purified PvTRAg protein show interactions with purified PvTRAg and native PvTRAg using Western blots and indirect enzyme-linked immunosorbent assay (ELISA). We also developed an antibody-antigen-based qualitative assay using biolayer interferometry (BLI) to detect vivax infection using plasma samples from patients with different febrile diseases and healthy controls. The polyclonal anti-PvTRAg antibodies were used to capture free native PvTRAg from the patient plasma samples using BLI, providing a new expansion range to make the assay quick, accurate, sensitive, and high-throughput. The data presented in this report provides a proof of concept for PvTRAg, a new antigen, for developing a diagnostic assay for P. vivax identification and differentiation from the rest of the Plasmodium species and, at a later stage, translating the BLI assay into affordable, point-of-care formats to make it more accessible.
Partial Bell-State Measurement with Type-II Parametric Down Conversion: Extracting Phase Information from the Zeropoint Field (I)
Authors: Casado Alberto , Guerra Santiago
Published: February 2023

In this paper, the nexus between the Bell-state measurement and extracting phase information from the zeropoint field is investigated. For this purpose, the Wigner representation in the Heisenberg picture is applied in a Bell-type experiment in which the polarisation-entangled photon pairs generated in a type-II parametric down-conversion do not overlap. The signal intensities at the detectors are calculated in a four-mode approximation, being expressed as functions of the modules and phases of the four zeropoint amplitudes entering the crystal. A general criterion for identifying the correlated detectors is proposed based on the equality of the signal intensities, and without involving the calculation of the joint detection probabilities. In addition, from the analyses in the rectilinear and diagonal basis, it is shown that the distinguishability of the polarisation Bell states, which is in direct correspondence with the joint detection events in each experiment, can be related to the knowledge of the phases of the vacuum field entering the entanglement source, and giving rise to correlated detections. To this purpose, it is conjectured that a detection event is associated with a maximum value of the signal intensity averaged in the modules of the zeropoint amplitudes, as a function of the vacuum phases.
Use of Digital Tools by English Language Schoolteachers
Authors: Moorhouse Benjamin Luke , Yan Lu
Published: February 2023

The present study explored the use of digital tools by English language schoolteachers in Hong Kong. Using a qualitative-dominant sequential mixed-methods design, wherein an online mixed-methods survey of primary and secondary school English language teachers (N = 83) and follow-up in-depth interviews (N = 22) were employed, this study collectively explored which digital tools the participants used, why the participants used these tools, and how the participants used them. Fourteen categories of tools utilized by teachers in their professional practice were identified. These were further categorized into core, additional, and remote digital tools based on their mode and frequency of use. Affordances of the core digital tools for language teaching were identified, with examples of teachers’ pedagogical uses of the tools presented. By providing an overview of digital tool use, teachers, school leadership, and professional development providers can better understand how teachers should be prepared for the digital realities of schools. In addition, this study provides a model for understanding the context and subject-specific bidirectional relationship between tools, their affordances, and teachers’ professional tasks.
Dealing with Teacher Shortage in Germany—A Closer View of Four Federal States
Authors: Seeliger Sandra , Håkansson Lindqvist Marcia
Published: February 2023

Teacher shortage can be said to be an international challenge. In this article, the phenomenon of teacher shortage in 4 of the 16 German states is explored and analysed. These four states were selected to demonstrate the variety and give examples of different approaches dealing with teacher shortage. Similarities and differences were observed and the most evident similarity is that all states that are presented have to deal with a shortage of teachers. Fluctuations of student numbers and waves of retirements, and increased need of new employment may be foreseen. However, the resources of the universities and teacher mobility between states is not always facilitated. The number of side entrants is remarkably low in some states. This may mean that their planning strategies are better or their recruitment for teacher training study places is more successful than in those states that need to employ side entrants on a regular basis. These are questions that cannot be answered yet. In conclusion, more research is called for to study how these results can be interpreted in order to allow the findings and their implications to be discussed in the broadest context possible.