bluuebunny/crossref_metadata_2025 huggingface dataset:
A Blockchain Solution for Securing Real Property Transactions: A Case Study for Serbia
Authors: Sladić Goran , Milosavljević Branko , Nikolić Siniša , Sladić Dubravka , Radulović Aleksandra
Published: January 2021

The origins of digital money and blockchain technology goes back to the 1980s, but in the last decade, the blockchain technology gained large popularity in the financial sector with the appearance of cryptocurrencies such as Bitcoin. However, recently, many other fields of application have been recognized, particularly with the development of smart contracts. Among them is the possible application of blockchain technology in the domain of land administration, mostly as a tool for transparency in the developing countries and means to fight corruption. However, developed countries also find interest in launching pilot projects to test their applicability in land administration domain for reasons such as to increase the speed and reduce costs of the real property transactions through a more secure environment. In this paper, we analyse how transactions are handled in Serbian land administration and how this process may be supported by modern ledger technologies such as blockchain. In order to analyse how blockchain could be implemented to support transactions in land information systems (LIS), it is necessary to understand cadastral processes and transactions in LIS, as well as legislative and organizational aspects of LIS. Transactions in cadastre comprise many actors and utilize both alphanumeric (descriptive or legal) data and geospatial data about property boundaries on the cadastral map. Based on the determined requirements for the blockchain-based LIS, we propose a system architecture for its implementation. Such a system keeps track of transactions in LIS in an immutable and tamper-proof manner to increase the security of the system and consequently increase the speed of transactions, efficiency, and data integrity without a significant impact on the existing laws and regulations. The system is anticipated as a permissioned public blockchain implemented on top of the Ethereum network.
Prussian Blue: A Safe Pigment with Zeolitic-Like Activity
Authors: Estelrich Joan , Busquets Maria Antònia
Published: January 2021

Prussian blue (PB) and PB analogues (PBA) are coordination network materials that present important similarities with zeolites concretely with their ability of adsorbing cations. Depending on the conditions of preparation, which is cheap and easy, PB can be classified into soluble PB and insoluble PB. The zeolitic-like properties are mainly inherent to insoluble form. This form presents some defects in its cubic lattice resulting in an open structure. The vacancies make PB capable of taking up and trapping ions or molecules into the lattice. Important adsorption characteristics of PB are a high specific area (370 m2 g−1 determined according the BET theory), uniform pore diameter, and large pore width. PB has numerous applications in many scientific and technological fields. PB are assembled into nanoparticles that, due to their biosafety and biocompatibility, can be used for biomedical applications. PB and PBA have been shown to be excellent sorbents of radioactive cesium and radioactive and nonradioactive thallium. Other cations adsorbed by PB are K+, Na+, NH4+, and some divalent cations. PB can also capture gaseous molecules, hydrocarbons, and even luminescent molecules such as 2-aminoanthracene. As the main adsorptive application of PB is the selective removal of cations from the environment, it is important to easily separate the sorbent of the purified solution. To facilitate this, PB is encapsulated into a polymer or coats a support, sometimes magnetic particles. Finally, is remarkable to point out that PB can be recycled and the adsorbed material can be recovered.
The Landscape of microRNAs in βCell: Between Phenotype Maintenance and Protection
Authors: Grieco Giuseppina Emanuela , Brusco Noemi , Licata Giada , Fignani Daniela , Formichi Caterina , Nigi Laura , Sebastiani Guido , Dotta Francesco
Published: January 2021

Diabetes mellitus is a group of heterogeneous metabolic disorders characterized by chronic hyperglycaemia mainly due to pancreatic β cell death and/or dysfunction, caused by several types of stress such as glucotoxicity, lipotoxicity and inflammation. Different patho-physiological mechanisms driving β cell response to these stresses are tightly regulated by microRNAs (miRNAs), a class of negative regulators of gene expression, involved in pathogenic mechanisms occurring in diabetes and in its complications. In this review, we aim to shed light on the most important miRNAs regulating the maintenance and the robustness of β cell identity, as well as on those miRNAs involved in the pathogenesis of the two main forms of diabetes mellitus, i.e., type 1 and type 2 diabetes. Additionally, we acknowledge that the understanding of miRNAs-regulated molecular mechanisms is fundamental in order to develop specific and effective strategies based on miRNAs as therapeutic targets, employing innovative molecules.
Transcriptional Analysis of C-Repeat Binding Factors in Fruit of Citrus Species with Differential Sensitivity to Chilling Injury during Postharvest Storage
Authors: Salvo Matías , Rey Florencia , Arruabarrena Ana , Gambetta Giuliana , Rodrigo María J. , Zacarías Lorenzo , Lado Joanna
Published: January 2021

Citrus fruit are sensitive to chilling injury (CI) during cold storage, a peel disorder that causes economic losses. C-repeat binding factors (CBFs) are related to cold acclimation and tolerance in different plants. To explore the role of Citrus CBFs in fruit response to cold, an in silico study was performed, revealing three genes (CBF1, CBF2, and CBF3) whose expression in CI sensitive and tolerant cultivars was followed. Major changes occurred at the early stages of cold exposure (1–5 d). Interestingly, CBF1 was the most stimulated gene in the peel of CI-tolerant cultivars (Lisbon lemon, Star Ruby grapefruit, and Navelina orange), remaining unaltered in sensitive cultivars (Meyer lemon, Marsh grapefruit, and Salustiana orange). Results suggest a positive association of CBF1 expression with cold tolerance in Citrus cultivars (except for mandarins), whereas the expression of CBF2 or CBF3 genes did not reveal a clear relationship with the susceptibility to CI. Light avoidance during fruit growth reduced postharvest CI in most sensitive cultivars, associated with a rapid and transient enhance in the expression of the three CBFs. Results suggest that CBFs-dependent pathways mediate at least part of the cold tolerance responses in sensitive Citrus, indicating that CBF1 participates in the natural tolerance to CI.
Comprehensive Overview of Gene Rearrangements in Childhood T-Cell Acute Lymphoblastic Leukaemia
Authors: Mroczek Anna , Zawitkowska Joanna , Kowalczyk Jerzy , Lejman Monika
Published: January 2021

Acute lymphoblastic leukaemia (ALL) is a relevant form of childhood neoplasm, as it accounts for over 80% of all leukaemia cases. T-cell ALL constitutes a genetically heterogeneous cancer derived from T-lymphoid progenitors. The diagnosis of T-ALL is based on morphologic, immunophenotypic, cytogenetic, and molecular features, thus the results are used for patient stratification. Due to the expression of surface and intracellular antigens, several subtypes of T-ALL can be distinguished. Although the aetiology of T-ALL remains unclear, a wide spectrum of rearrangements and mutations affecting crucial signalling pathways has been described so far. Due to intensive chemotherapy regimens and supportive care, overall cure rates of more than 80% in paediatric T-ALL patients have been accomplished. However, improved knowledge of the mechanisms of relapse, drug resistance, and determination of risk factors are crucial for patients in the high-risk group. Even though some residual disease studies have allowed the optimization of therapy, the identification of novel diagnostic and prognostic markers is required to individualize therapy. The following review summarizes our current knowledge about genetic abnormalities in paediatric patients with T-ALL. As molecular biology techniques provide insights into the biology of cancer, our study focuses on new potential therapeutic targets and predictive factors which may improve the outcome of young patients with T-ALL.
Pulsed Electromagnetic Field Stimulation in Osteogenesis and Chondrogenesis: Signaling Pathways and Therapeutic Implications
Authors: Varani Katia , Vincenzi Fabrizio , Pasquini Silvia , Blo Irene , Salati Simona , Cadossi Matteo , De Mattei Monica
Published: January 2021

Mesenchymal stem cells (MSCs) are the main cell players in tissue repair and thanks to their self-renewal and multi-lineage differentiation capabilities, they gained significant attention as cell source for tissue engineering (TE) approaches aimed at restoring bone and cartilage defects. Despite significant progress, their therapeutic application remains debated: the TE construct often fails to completely restore the biomechanical properties of the native tissue, leading to poor clinical outcomes in the long term. Pulsed electromagnetic fields (PEMFs) are currently used as a safe and non-invasive treatment to enhance bone healing and to provide joint protection. PEMFs enhance both osteogenic and chondrogenic differentiation of MSCs. Here, we provide extensive review of the signaling pathways modulated by PEMFs during MSCs osteogenic and chondrogenic differentiation. Particular attention has been given to the PEMF-mediated activation of the adenosine signaling and their regulation of the inflammatory response as key player in TE approaches. Overall, the application of PEMFs in tissue repair is foreseen: (1) in vitro: to improve the functional and mechanical properties of the engineered construct; (2) in vivo: (i) to favor graft integration, (ii) to control the local inflammatory response, and (iii) to foster tissue repair from both implanted and resident MSCs cells.
Crosstalk of the Caspase Family and Mammalian Target of Rapamycin Signaling
Authors: Yan Junfang , Xie Yi , Si Jing , Gan Lu , Li Hongyan , Sun Chao , Di Cuixia , Zhang Jinhua , Huang Guomin , Zhang Xuetian , Zhang Hong
Published: January 2021

Cell can integrate the caspase family and mammalian target of rapamycin (mTOR) signaling in response to cellular stress triggered by environment. It is necessary here to elucidate the direct response and interaction mechanism between the two signaling pathways in regulating cell survival and determining cell fate under cellular stress. Members of the caspase family are crucial regulators of inflammation, endoplasmic reticulum stress response and apoptosis. mTOR signaling is known to mediate cell growth, nutrition and metabolism. For instance, over-nutrition can cause the hyperactivation of mTOR signaling, which is associated with diabetes. Nutrition deprivation can inhibit mTOR signaling via SH3 domain-binding protein 4. It is striking that Ras GTPase-activating protein 1 is found to mediate cell survival in a caspase-dependent manner against increasing cellular stress, which describes a new model of apoptosis. The components of mTOR signaling-raptor can be cleaved by caspases to control cell growth. In addition, mTOR is identified to coordinate the defense process of the immune system by suppressing the vitality of caspase-1 or regulating other interferon regulatory factors. The present review discusses the roles of the caspase family or mTOR pathway against cellular stress and generalizes their interplay mechanism in cell fate determination.
Dynamic Changes in the Ability to Release Neutrophil ExtraCellular Traps in the Course of Childhood Acute Leukemias
Authors: Ostafin Magdalena , Ciepiela Olga , Pruchniak Michał , Wachowska Małgorzata , Ulińska Edyta , Mrówka Piotr , Głodkowska-Mrówka Eliza , Demkow Urszula
Published: January 2021

Acute leukemias, the most common cancers in children, are characterized by excessive proliferation of malignant progenitor cells. As a consequence of impaired blood cell production, leukemia patients are susceptible to infectious complications—a major cause of non-relapse mortality. Neutrophil extracellular traps (NETs) are involved in various pathologies, from autoimmunity to cancer. Although aberrant NETs formation may be partially responsible for immune defects observed in acute leukemia, still little is known on the NET release in the course of leukemia. Here, we present the first comprehensive evaluation of NETs formation by neutrophils isolated from children with acute leukemia in different stages of the disease and treatment stimulated in vitro with phorbol 12-myristate 13-acetate (PMA), N-formyl-methionyl-leucyl-phenylalanine (fMLP), and calcium ionophore (CI). NETs release was measured using quantitative fluorescent method and visualized microscopically. In this setting, NETs release was significantly impaired in leukemic children both at the diagnosis and during the treatment, and full restoration of neutrophil function was achieved only after successful completion of the leukemia treatment. We suggest that neutrophil function impairment may result from both disease- and treatment-related factors. In this context, deficient innate immune response observed in acute leukemia patients may be present regardless of neutrophil count and contribute to secondary immunodeficiency observed in this population.
High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee
Authors: Wang Xiaofei , Zhong Zhaopeng , Chen Xiangyin , Hong Ziyun , Lin Weimin , Mu Xiaohuan , Hu Xiaosong , Zheng Hao
Published: January 2021

HFD (high-fat diet) induces obesity and metabolic disorders, which is associated with the alteration in gut microbiota profiles. However, the underlying molecular mechanisms of the processes are poorly understood. In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota. Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees. However, microbiota-free honey bees reared on high-fat diets did not significantly change their phenotypes. Different fatty acid compositions in palm and soybean oil altered the lipid profiles of the honey bee body. Remarkably, dietary fats regulated lipid metabolism and immune-related gene expression at the transcriptional level. Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats. Moreover, a high-fat diet increased the relative abundance of Gilliamella, while the level of Bartonella was significantly decreased in palm oil groups. This study establishes a novel honey bee model of studying the crosstalk between dietary fat, gut microbiota, and host metabolism.
A Single Dose of Psilocybin Increases Synaptic Density and Decreases 5-HT2A Receptor Density in the Pig Brain
Authors: Raval Nakul Ravi , Johansen Annette , Donovan Lene Lundgaard , Ros Nídia Fernandez , Ozenne Brice , Hansen Hanne Demant , Knudsen Gitte Moos
Published: January 2021

A single dose of psilocybin, a psychedelic and serotonin 2A receptor (5-HT2AR) agonist, may be associated with antidepressant effects. The mechanism behind its antidepressive action is unknown but could be linked to increased synaptogenesis and down-regulation of cerebral 5-HT2AR. Here, we investigate if a single psychedelic dose of psilocybin changes synaptic vesicle protein 2A (SV2A) and 5-HT2AR density in the pig brain. Twenty-four awake pigs received either 0.08 mg/kg psilocybin or saline intravenously. Twelve pigs (n = 6/intervention) were euthanized one day post-injection, while the remaining twelve pigs were euthanized seven days post-injection (n = 6/intervention). We performed autoradiography on hippocampus and prefrontal cortex (PFC) sections with [3H]UCB-J (SV2A), [3H]MDL100907 (5-HT2AR antagonist) and [3H]Cimbi-36 (5-HT2AR agonist). One day post psilocybin injection, we observed 4.42% higher hippocampal SV2A density and lowered hippocampal and PFC 5-HT2AR density (−15.21% to −50.19%). These differences were statistically significant in the hippocampus for all radioligands and in the PFC for [3H]Cimbi-36 only. Seven days post-intervention, there was still significantly higher SV2A density in the hippocampus (+9.24%) and the PFC (+6.10%), whereas there were no longer any differences in 5-HT2AR density. Our findings suggest that psilocybin causes increased persistent synaptogenesis and an acute decrease in 5-HT2AR density, which may play a role in psilocybin’s antidepressive effects.
The Effect of Hypoxia on the Expression of CXC Chemokines and CXC Chemokine Receptors—A Review of Literature
Authors: Korbecki Jan , Kojder Klaudyna , Kapczuk Patrycja , Kupnicka Patrycja , Gawrońska-Szklarz Barbara , Gutowska Izabela , Chlubek Dariusz , Baranowska-Bosiacka Irena
Published: January 2021

Hypoxia is an integral component of the tumor microenvironment. Either as chronic or cycling hypoxia, it exerts a similar effect on cancer processes by activating hypoxia-inducible factor-1 (HIF-1) and nuclear factor (NF-κB), with cycling hypoxia showing a stronger proinflammatory influence. One of the systems affected by hypoxia is the CXC chemokine system. This paper reviews all available information on hypoxia-induced changes in the expression of all CXC chemokines (CXCL1, CXCL2, CXCL3, CXCL4, CXCL5, CXCL6, CXCL7, CXCL8 (IL-8), CXCL9, CXCL10, CXCL11, CXCL12 (SDF-1), CXCL13, CXCL14, CXCL15, CXCL16, CXCL17) as well as CXC chemokine receptors—CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6, CXCR7 and CXCR8. First, we present basic information on the effect of these chemoattractant cytokines on cancer processes. We then discuss the effect of hypoxia-induced changes on CXC chemokine expression on the angiogenesis, lymphangiogenesis and recruitment of various cells to the tumor niche, including myeloid-derived suppressor cells (MDSCs), tumor-associated macrophages (TAMs), tumor-associated neutrophils (TANs), regulatory T cells (Tregs) and tumor-infiltrating lymphocytes (TILs). Finally, the review summarizes data on the use of drugs targeting the CXC chemokine system in cancer therapies.
Elucidating the Functional Roles of Helper and Cytotoxic T Cells in the Cell-Mediated Immune Responses of Olive Flounder (Paralichthys olivaceus)
Authors: Jung Jae Wook , Lee Ae Rin , Kim Jaesung , Kim Young Rim , Lazarte Jassy Mary S. , Lee Jung Suk , Thompson Kim D. , Kim Hyeongsu , Jung Tae Sung
Published: January 2021

In higher vertebrates, helper and cytotoxic T cells, referred to as CD4 and CD8 T lymphocytes, respectively, are mainly associated with adaptive immunity. The adaptive immune system in teleosts involves T cells equivalent to those found in mammals. We previously generated monoclonal antibodies (mAbs) against olive flounder (Paralichthys olivaceus) CD4 T cells, CD4-1 and CD4-2, and used these to describe the olive flounder’s CD4 Tcell response during a viral infection. In the present study, we successfully produced mAbs against CD8 T lymphocytes and their specificities were confirmed using immuno-blotting, immunofluorescence staining, flow cytometry analysis andreverse transcription polymerase chain reaction (RT-PCR). The results showed that these mAbs are specific for CD8 T lymphocytes. We also investigated variations in CD4 and CD8 T cells populations, and analyzed the expression of immune-related genes expressed by these cells in fish infected with nervous necrosis virus or immunized with thymus dependent and independent antigens. We found that both CD4 and CD8 T lymphocyte populations significantly increased in these fish and Th1-related genes were up-regulated compared to the control group. Collectively, these findings suggest that the CD4 and CD8 T lymphocytes in olive flounder are similar to the helper and cytotoxic T cells found in mammals, and Th1 and cytotoxic immune responses are primarily involved in the early adaptive immune response against extracellular antigens.
The Prognostic Value of Eosinophil Recovery in COVID-19: A Multicentre, Retrospective Cohort Study on Patients Hospitalised in Spanish Hospitals
Authors: Mateos González María , Sierra Gonzalo Elena , Casado Lopez Irene , Arnalich Fernández Francisco , Beato Pérez José Luis , Monge Monge Daniel , Vargas Núñez Juan Antonio , García Fenoll Rosa , Suárez Fernández Carmen , Freire Castro Santiago Jesús , Mendez Bailon Manuel , Perales Fraile Isabel , Madrazo Manuel , Pesqueira Fontan Paula Maria , Magallanes Gamboa Jeffrey Oskar , González García Andrés , Crestelo Vieitez Anxela , Fonseca Aizpuru Eva María , Aranguren Arostegui Asier , Coduras Erdozain Ainara , Martinez Cilleros Carmen , Loureiro Amigo Jose , Epelde Francisco , Lumbreras Bermejo Carlos , Antón Santos Juan Miguel , for the SEMI-COVID-19 Network
Published: January 2021

Objectives: A decrease in blood cell counts, especially lymphocytes and eosinophils, has been described in patients with serious Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), but there is no knowledge of their potential role of the recovery in these patients’ prognosis. This article aims to analyse the effect of blood cell depletion and blood cell recovery on mortality due to COVID-19. Design: This work was a retrospective, multicentre cohort study of 9644 hospitalised patients with confirmed COVID-19 from the Spanish Society of Internal Medicine’s SEMI-COVID-19 Registry. Setting: This study examined patients hospitalised in 147 hospitals throughout Spain. Participants: This work analysed 9644 patients (57.12% male) out of a cohort of 12,826 patients ≥18 years of age hospitalised with COVID-19 in Spain included in the SEMI-COVID-19 Registry as of 29 May 2020. Main outcome measures: The main outcome measure of this work is the effect of blood cell depletion and blood cell recovery on mortality due to COVID-19. Univariate analysis was performed to determine possible predictors of death, and then multivariate analysis was carried out to control for potential confounders. Results: An increase in the eosinophil count on the seventh day of hospitalisation was associated with a better prognosis, including lower mortality rates (5.2% vs. 22.6% in non-recoverers, OR 0.234; 95% CI, 0.154 to 0.354) and lower complication rates, especially regarding the development of acute respiratory distress syndrome (8% vs. 20.1%, p = 0.000) and ICU admission (5.4% vs. 10.8%, p = 0.000). Lymphocyte recovery was found to have no effect on prognosis. Treatment with inhaled or systemic glucocorticoids was not found to be a confounding factor. Conclusion: Eosinophil recovery in patients with COVID-19 who required hospitalisation had an independent prognostic value for all-cause mortality and a milder course.
Efficacy of Computer-Aided Static Navigation Technique on the Accuracy of Endodontic Microsurgery. A Systematic Review and Meta-Analysis
Authors: Zubizarreta-Macho Álvaro , Castillo-Amature César , Montiel-Company José María , Mena-Álvarez Jesús
Published: January 2021

The aim of this systematic review and meta-analysis was to analyze the efficacy of the computer-aided static navigation technique on the accuracy of root apex location in endodontic microsurgery. Material and Methods: A systematic literature review and meta-analysis, based on Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) recommendations, of clinical studies that evaluated the apex location rate of the computer-aided static navigation techniques applied to endodontic microsurgery. A total of four databases were consulted in the literature search: Pubmed-Medline, Scopus, Cochrane, and Web of Science. After eliminating duplicated articles and applying the inclusion criteria, seven articles were selected for the qualitative and the quantitative analysis. Results: The root apex location success rate stated at 96.8% (confidence interval (CI): 93.0–100%) of the cases performed through a computer-aided static navigation technique. The prediction interval ranges from 91.4% to 100%. The meta-analysis did not detect heterogeneity between the combined studies (Q-test = 6.15; p-value = 0.407; I2 = 2.4%). The computer-aided static navigation techniques showed a root apex location success rate 27 times higher than conventional endodontic microsurgery procedures (Q test = 0.80; p = 0.671; I2 = 0%). Three studies of computer-aided static navigation techniques and control group were compared using a random effects model with the Mantel-Haenszel method with a statistically significant odds success ratio of 27.7, with a 95% confidence interval between 11.3 and 68.1 (z test = 7.23; p < 0.0001). Conclusions: According to in vitro studies analyzed, endodontic microsurgeries performed through computer-aided static navigation techniques show a high precision.
Improved Acquisition and Reconstruction for Wavelength-Resolved Neutron Tomography
Authors: Venkatakrishnan Singanallur , Zhang Yuxuan , Dessieux Luc , Hoffmann Christina , Bingham Philip , Bilheux Hassina
Published: January 2021

Wavelength-resolved neutron tomography (WRNT) is an emerging technique for characterizing samples relevant to the materials sciences in 3D. WRNT studies can be carried out at beam lines in spallation neutron or reactor-based user facilities. Because of the limited availability of experimental time, potential imperfections in the neutron source, or constraints placed on the acquisition time by the type of sample, the data can be extremely noisy resulting in tomographic reconstructions with significant artifacts when standard reconstruction algorithms are used. Furthermore, making a full tomographic measurement even with a low signal-to-noise ratio can take several days, resulting in a long wait time before the user can receive feedback from the experiment when traditional acquisition protocols are used. In this paper, we propose an interlaced scanning technique and combine it with a model-based image reconstruction algorithm to produce high-quality WRNT reconstructions concurrent with the measurements being made. The interlaced scan is designed to acquire data so that successive measurements are more diverse in contrast to typical sequential scanning protocols. The model-based reconstruction algorithm combines a data-fidelity term with a regularization term to formulate the wavelength-resolved reconstruction as minimizing a high-dimensional cost-function. Using an experimental dataset of a magnetite sample acquired over a span of about two days, we demonstrate that our technique can produce high-quality reconstructions even during the experiment compared to traditional acquisition and reconstruction techniques. In summary, the combination of the proposed acquisition strategy with an advanced reconstruction algorithm provides a novel guideline for designing WRNT systems at user facilities.
Cryptococcus bacillisporus (VGIII) Meningoencephalitis Acquired in Santa Cruz, Bolivia
Authors: Thompson Luis , Porte Lorena , Díaz Violeta , Díaz María Cristina , Solar Sebastián , Valenzuela Pablo , Norley Nicole , Pires Yumai , Carreño Fernando , Valenzuela Sergio , Shabani Rukmane , Rickerts Volker , Weitzel Thomas
Published: January 2021

We describe a case of chronic meningoencephalitis with hydrocephalus caused by Cryptococcus bacillisporus (VGIII) in an immunocompetent patient from Santa Cruz, Bolivia. This first report of a member of the Cryptococcus gattii species complex from Bolivia suggests that C. bacillisporus (VGIII) is present in this tropical region of the country and complements our epidemiological and clinical knowledge of this group of emerging fungal pathogens in South America.
The Effect of Enrichment Filling and Engagement Time on Regurgitation and Reingestion Behaviour in Three Zoo-Housed Orangutans
Authors: Nash Rebecca , Johnston Helen , Robbins Amy , Descovich Kris
Published: January 2021

Regurgitation and reingestion (R/R) is a prevalent, abnormal behaviour observed in captive great apes. R/R may be related to animal welfare and while less R/R appears to occur when apes are provided with browse and continuous foraging opportunities, the aetiology of the behaviour (e.g., foraging time or taste characteristics such as sweetness) is not well understood. This study aimed to determine how environmental enrichment may affect R/R in three zoo-housed, adult orangutans. Over eight weeks, nine fillable enrichment items were provided twice to each orangutan–once with a sweet filling and once with a savoury filling. Enrichment engagement time and R/R behaviour were monitored for 1-h after the item was provided. Individual differences were found in R/R occurrence. One individual was more likely to perform R/R when given enrichment with a sweet filling (p < 0.05), and a second was more likely to R/R with savoury filled enrichment (p < 0.05). R/R behaviour from the third orangutan was unaffected by enrichment filling (p > 0.05), however he engaged longer with savoury filled enrichment, compared to sweet (p < 0.05). No relationship was found between engagement time and amount of R/R behaviour, for any of the orangutans (p > 0.05). While these results should not be generalized without a larger study, they do suggest that diet and enrichment qualities may play a role in the performance of R/R, and individual variation should not be overlooked when considering causation.
Rural Development from a Gender Perspective: The Case of Women Farmers in Southern Spain
Authors: De Pablo Valenciano Jaime , Milán-García Juan , Uribe-Toril Juan , Guerrero-Villalba María Angustias
Published: January 2021

This article analyses the contribution to local development by women workers in the fruit- and vegetable-handling sector in Almería (Spain) over the last five years (2015–2019). It is a continuation of research carried out during the period 2000–2014. Using data collected through surveys and focus groups, the aim is to ascertain if the results obtained in this analysis meet the condition of sustainability, i.e., whether the improvement in working women’s quality of life has been maintained over time, and whether these beneficial effects have multiplied. The results show that women workers in the fruit- and vegetable-handling sector are satisfied with their jobs and with the company they are working for. The existence of fixed-discontinuous employment contracts facilitates greater flexibility for women in terms of balancing work and family life. This main contribution of this study lies in extrapolating the sustainability of a local development model in regard to other initiatives that aim to increase women’s empowerment in the labour market.
Mitochondrial Functionality in Inflammatory Pathology-Modulatory Role of Physical Activity
Authors: Casuso Rafael A. , Huertas Jesús R.
Published: January 2021

The incidence and severity of metabolic diseases can be reduced by introducing healthy lifestyle habits including moderate exercise. A common observation in age-related metabolic diseases is an increment in systemic inflammation (the so-called inflammaging) where mitochondrial reactive oxygen species (ROS) production may have a key role. Exercise prevents these metabolic pathologies, at least in part, due to its ability to alter immunometabolism, e.g., reducing systemic inflammation and by improving immune cell metabolism. Here, we review how exercise regulates immunometabolism within contracting muscles. In fact, we discuss how circulating and resident macrophages alter their function due to mitochondrial signaling, and we propose how these effects can be triggered within skeletal muscle in response to exercise. Finally, we also describe how exercise-induced mitochondrial adaptations can help to fight against virus infection. Moreover, the fact that moderate exercise increases circulating immune cells must be taken into account by public health agencies, as it may help prevent virus spread. This is of interest in order to face not only acute respiratory-related coronavirus (SARS-CoV) responsible for the COVID-19 pandemic but also for future virus infection challenges.
Fly-Ash-Based Geopolymers Reinforced by Melamine Fibers
Authors: Kozub Barbara , Bazan Patrycja , Mierzwiński Dariusz , Korniejenko Kinga
Published: January 2021

This paper presents the results of research on geopolymer composites based on fly ash with the addition of melamine fibers in amounts of 0.5%, 1% and 2% by weight and, for comparison, without the addition of fibers. The melamine fibers used in the tests retain their melamine resin properties by 100% and are characterized by excellent acoustic and thermal insulation as well as excellent filtration. In addition, these fibers are nonflammable, resistant to chemicals, resistant to UV radiation, characterized by high temperature resistance and, most importantly, do not show thermal-related shrinking, melting and dripping. This paper presents the results of density measurements, compressive and flexural strength as well as the results of the measurement of thermal radiation changes in samples subjected to a temperature of 600 °C. The results indicate that melamine fibers can be used as geopolymer reinforcement. The best result was achieved for 0.5% by weight amount of reinforcement, approximately 53 MPa, compared to 41 MPa for a pure matrix. In the case of flexural strength, the best results were obtained for the samples made of unreinforced geopolymer and samples with the addition of 0.5% by weight of melamine fibers, which were characterized by bending strength values above 9 MPa, amounting to 10.7 MPa and 9.3 MPa, respectively. The thermal radiation measurements and fire-jet test did not confirm the increasing thermal and fire resistance of the composites reinforced by melamine fiber.
Flexible Phase Change Material Fiber: A Simple Route to Thermal Energy Control Textiles
Authors: Yan Yurong , Li Weipei , Zhu Ruitian , Lin Chao , Hufenus Rudolf
Published: January 2021

A flexible hollow polypropylene (PP) fiber was filled with the phase change material (PCM) polyethylene glycol 1000 (PEG1000), using a micro-fluidic filling technology. The fiber’s latent heat storage and release, thermal reversibility, mechanical properties, and phase change behavior as a function of fiber drawing, were characterized. Differential scanning calorimetry (DSC) results showed that both enthalpies of melting and solidification of the PCM encased within the PP fiber were scarcely influenced by the constraint, compared to unconfined PEG1000. The maximum filling ratio of PEG1000 within the tubular PP filament was ~83 wt.%, and the encapsulation efficiencies and heat loss percentages were 96.7% and 7.65% for as-spun fibers and 93.7% and 1.53% for post-drawn fibers, respectively. Weak adherence of PEG on the inner surface of the PP fibers favored bubble formation and aggregating at the core–sheath interface, which led to different crystallization behavior of PEG1000 at the interface and in the PCM matrix. The thermal stability of PEG was unaffected by the PP encasing; only the decomposition temperature, corresponding to 50% weight loss of PEG1000 inside the PP fiber, was a little higher compared to that of pure PEG1000. Cycling heating and cooling tests proved the reversibility of latent heat release and storage properties, and the reliability of the PCM fiber.
A Numerical Analysis of Resin Flow in Woven Fabrics: Effect of Local Tow Curvature on Dual-Scale Permeability
Authors: Alotaibi Hatim , Jabbari Masoud , Soutis Constantinos
Published: January 2021

Permeability is a crucial flow parameter in liquid composite moulding (LCM), which is required to predict fibre impregnation, void formation and resin back flow. This work investigates the dual-scale (micro- and meso-) nature of permeability during resin infusion into woven fabric by incorporating the intra tow flow where the degree of local tow curvature (tow/yarn undulation) is taken into account. The mesoscopic permeability of a dual-scale porous media in a unit cell is estimated using Darcy’s law, where the Gebart analytical model is applied for the intra tow flow in longitudinal and transverse directions with respect to distinct fibre packing arrangements. The results suggest that for a low fibre volume fraction (≤42%), the degree of local curvature at the mesoscale can be neglected. However, for a high fibre volume fraction (>42%) and a higher fibre bundle curvature, the proposed model should be adopted, since the resin flow is affected by a mesoscopic tow curvature that could result in around 14% error in predicting permeability. It is shown that the permeability results of the current study are in good agreement with and in the range of the retrieved available experimental data from the literature.
Investigation and Optimization of the SLM and WEDM Processes’ Parameters for the AlSi10Mg-Sintered Part
Authors: Franczyk Emilia , Machno Magdalena , Zębala Wojciech
Published: January 2021

Presented study concerns the issue of processing the AlSi10Mg aluminum alloy with a use of WEDM technology. Two types of samples tested during the experiment were previously produced in SLM and in casting processes. The aim of the research was to determine the dependence of the input parameters of SLM (laser scanning speed) and WEDM (current amplitude) processes on the performance of the WEDM process as well as on the roughness of the cut surfaces. The experiment was carried out on a specially prepared test stand, and the results’ analysis was carried out using the ANOVA (analysis of variance). A strong influence of the WEDM current on the process speed and on the Ra and Rz roughness parameters of the produced samples was found. The effect of SLM laser scanning speed was not so strong, but it tended to be uniform. On the other hand, the influence of the tested parameters on the WEDM process energy turned out to be insignificant and irregular. It was also found that for the WEDM process a sample made in SLM technology with relatively high laser scanning speed may be a better choice than the cast one. A case study was carried out to optimize the parameters of the tested processes.
Dual Ion Releasing Nanoparticles for Modulating Osteogenic Cellular Microenvironment of Human Mesenchymal Stem Cells
Authors: Kim Yu-Jin , Lee Jaeyoung , Im Gwang-Bum , Song Jihun , Song Jiwoo , Chung Jiyong , Yu Taekyung , Bhang Suk Ho
Published: January 2021

In this study we developed a dual therapeutic metal ion-releasing nanoparticle for advanced osteogenic differentiation of stem cells. In order to enhance the osteogenic differentiation of human mesenchymal stem cells (hMSCs) and induce angiogenesis, zinc (Zn) and iron (Fe) were synthesized together into a nanoparticle with a pH-sensitive degradation property. Zn and Fe were loaded within the nanoparticles to promote early osteogenic gene expression and to induce angiogenic paracrine factor secretion for hMSCs. In vitro studies revealed that treating an optimized concentration of our zinc-based iron oxide nanoparticles to hMSCs delivered Zn and Fe ion in a controlled release manner and supported osteogenic gene expression (RUNX2 and alkaline phosphatase) with improved vascular endothelial growth factor secretion. Simultaneous intracellular release of Zn and Fe ions through the endocytosis of the nanoparticles further modulated the mild reactive oxygen species generation level in hMSCs without cytotoxicity and thus improved the osteogenic capacity of the stem cells. Current results suggest that our dual ion releasing nanoparticles might provide a promising platform for future biomedical applications.
The Antibacterial Effects of Resin-Based Dental Sealants: A Systematic Review of In Vitro Studies
Authors: AlShahrani Saad Saeed , AlAbbas Mana’a Saleh , Garcia Isadora Martini , AlGhannam Maha Ibrahim , AlRuwaili Muath Abdulrahman , Collares Fabrício Mezzomo , Ibrahim Maria Salem
Published: January 2021

This review aimed to assess the antimicrobial effects of different antibacterial agents/compounds incorporated in resin-based dental sealants. Four databases (PubMed, MEDLINE, Web of Science and Scopus) were searched. From the 8052 records retrieved, 275 records were considered eligible for full-text screening. Nineteen studies met the inclusion criteria. Data extraction and quality assessment was performed by two independent reviewers. Six of the nineteen included studies were judged to have low risk of bias, and the rest had medium risk of bias. Compounds and particles such as zinc, tin, Selenium, chitosan, chlorhexidine, fluoride and methyl methacrylate were found to be effective in reducing the colony-forming unit counts, producing inhibition zones, reducing the optical density, reducing the metabolic activities, reducing the lactic acid and polysaccharide production and neutralizing the pH when they are added to the resin-based dental sealants. In addition, some studies showed that the antibacterial effect was not significantly different after 2 weeks, 2 months and 6 months aging in distilled water or phosphate-buffered saline. In conclusion, studies have confirmed the effectiveness of adding antibacterial agents/compounds to dental sealants. However, we should consider that these results are based on laboratory studies with a high degree of heterogeneity.
Investigation into the Effect of Cutting Conditions in Turning on the Surface Properties of Filament Winding GFRP Pipe Rings
Authors: Mansour Gabriel , Kyratsis Panagiotis , Korlos Apostolos , Tzetzis Dimitrios
Published: January 2021

There are numerous engineering applications where Glass Fiber Reinforced Polymer (GFRP) composite tubes are utilized, such as desalination plants, power transmission systems, and paper mill, as well as marine, industries. Some type of machining is required for those various applications either for joining or fitting procedures. Machining of GFRP has certain difficulties that may damage the tube itself because of fiber delamination and pull out, as well as matrix deboning. Additionally, short machining tool life may be encountered while the formation of powder like chips maybe relatively hazardous. The present paper investigates the effect of process parameters for surface roughness of glass fiber-reinforced polymer composite pipes manufactured using the filament winding process. Experiments were conducted based on the high-speed turning Computer Numerical Control (CNC) machine using Poly-Crystalline Diamond (PCD) tool. The process parameters considered were cutting speed, feed, and depth of cut. Mathematical models for the surface roughness were developed based on the experimental results, and Analysis of Variance (ANOVA) has been performed with a confidence level of 95% for validation of the models.
Dynamic Graph Learning: A Structure-Driven Approach
Authors: Jiang Bo , Huang Yuming , Panahi Ashkan , Yu Yiyi , Krim Hamid , Smith Spencer L.
Published: January 2021

The purpose of this paper is to infer a dynamic graph as a global (collective) model of time-varying measurements at a set of network nodes. This model captures both pairwise as well as higher order interactions (i.e., more than two nodes) among the nodes. The motivation of this work lies in the search for a connectome model which properly captures brain functionality across all regions of the brain, and possibly at individual neurons. We formulate it as an optimization problem, a quadratic objective functional and tensor information of observed node signals over short time intervals. The proper regularization constraints reflect the graph smoothness and other dynamics involving the underlying graph’s Laplacian, as well as the time evolution smoothness of the underlying graph. The resulting joint optimization is solved by a continuous relaxation of the weight parameters and an introduced novel gradient-projection scheme. While the work may be applicable to any time-evolving data set (e.g., fMRI), we apply our algorithm to a real-world dataset comprising recorded activities of individual brain cells. The resulting model is shown to be not only viable but also efficiently computable.
Four-Fold Formal Concept Analysis Based on Complete Idempotent Semifields
Authors: Valverde-Albacete Francisco José , Peláez-Moreno Carmen
Published: January 2021

Formal Concept Analysis (FCA) is a well-known supervised boolean data-mining technique rooted in Lattice and Order Theory, that has several extensions to, e.g., fuzzy and idempotent semirings. At the heart of FCA lies a Galois connection between two powersets. In this paper we extend the FCA formalism to include all four Galois connections between four different semivectors spaces over idempotent semifields, at the same time. The result is K¯-four-fold Formal Concept Analysis (K¯-4FCA) where K¯ is the idempotent semifield biasing the analysis. Since complete idempotent semifields come in dually-ordered pairs—e.g., the complete max-plus and min-plus semirings—the basic construction shows dual-order-, row–column- and Galois-connection-induced dualities that appear simultaneously a number of times to provide the full spectrum of variability. Our results lead to a fundamental theorem of K¯-four-fold Formal Concept Analysis that properly defines quadrilattices as 4-tuples of (order-dually) isomorphic lattices of vectors and discuss its relevance vis-à-vis previous formal conceptual analyses and some affordances of their results.
Preliminary Evaluation of 3D Printed Chitosan/Pectin Constructs for Biomedical Applications
Authors: Michailidou Georgia , Terzopoulou Zoe , Kehagia Argyroula , Michopoulou Anna , Bikiaris Dimitrios N.
Published: January 2021

In the present study, chitosan (CS) and pectin (PEC) were utilized for the preparation of 3D printable inks through pneumatic extrusion for biomedical applications. CS is a polysaccharide with beneficial properties; however, its printing behavior is not satisfying, rendering the addition of a thickening agent necessary, i.e., PEC. The influence of PEC in the prepared inks was assessed through rheological measurements, altering the viscosity of the inks to be suitable for 3D printing. 3D printing conditions were optimized and the effect of different drying procedures, along with the presence or absence of a gelating agent on the CS-PEC printed scaffolds were assessed. The mean pore size along with the average filament diameter were measured through SEM micrographs. Interactions among the characteristic groups of the two polymers were evident through FTIR spectra. Swelling and hydrolysis measurements confirmed the influence of gelation and drying procedure on the subsequent behavior of the scaffolds. Ascribed to the beneficial pore size and swelling behavior, fibroblasts were able to survive upon exposure to the ungelated scaffolds.