bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Effects of Low-Intensity Shock Wave Therapy on L6 Dorsal Root Ganglion/Spinal Cord and Blood Oxygenation Level-Dependent (BOLD) Functional Magnetic Resonance Imaging (fMRI) Changes in Capsaicin-Induced Prostatitis Rat Models
Authors: Wang Hung-Jen , Su Chia-Hao , Chen Yu-Ming , Yu Chun-Chieh , Chuang Yao-Chi
Published: April 2022

Neurogenic inflammation and central sensitization play a role in chronic prostatitis/chronic pelvic pain syndrome. We explore the molecular effects of low-intensity shock wave therapy (Li-ESWT) on central sensitization in a capsaicin-induced prostatitis rat model. Male Sprague–Dawley rats underwent intraprostatic capsaicin (10 mM, 0.1 cm3) injections. After injection, the prostate received Li-ESWT twice, one day apart. The L6 dorsal root ganglion (DRG)/spinal cord was harvested for histology and Western blotting on days 3 and 7. The brain blood oxygenation level-dependent (BOLD) functional images were evaluated using 9.4 T fMRI before the Li-ESWT and one day after. Intraprostatic capsaicin injection induced increased NGF-, BDNF-, and COX-2-positive neurons in the L6 DRG and increased COX-2, NGF, BDNF, receptor Trk-A, and TRPV1 protein expression in the L6 DRG and the dorsal horn of the L6 spinal cord, whose effects were significantly downregulated after Li-ESWT on the prostate. Intraprostatic capsaicin injection increased activity of BOLD fMRI responses in brain regions associated with pain-related responses, such as the caudate putamen, periaqueductal gray, and thalamus, whose BOLD signals were reduced after Li-ESWT. These findings suggest a potential mechanism of Li-ESWT on modulation of peripheral and central sensitization for treating CP/CPPS.
Overexpression of Rice BSR2 Confers Disease Resistance and Induces Enlarged Flowers in Torenia fournieri Lind
Authors: Maeda Satoru , Sasaki Katsutomo , Kaku Hisatoshi , Kanda Yasukazu , Ohtsubo Norihiro , Mori Masaki
Published: April 2022

Plant pathogens evade basal defense systems and attack different organs and tissues of plants. Genetic engineering of plants with genes that confer resistance against pathogens is very effective in pathogen control. Conventional breeding for disease resistance in ornamental crops is difficult and lagging relative to that in non-ornamental crops due to an inadequate number of disease-resistant genes. Therefore, genetic engineering of these plants with defense-conferring genes is a practical approach. We used rice BSR2 encoding CYP78A15 for developing transgenic Torenia fournieri Lind. lines. The overexpression of BSR2 conferred resistance against two devastating fungal pathogens, Rhizoctonia solani and Botrytis cinerea. In addition, BSR2 overexpression resulted in enlarged flowers with enlarged floral organs. Histological observation of the petal cells suggested that the enlargement in the floral organs could be due to the elongation and expansion of the cells. Therefore, the overexpression of BSR2 confers broad-spectrum disease resistance and induces the production of enlarged flowers simultaneously. Therefore, this could be an effective strategy for developing ornamental crops that are disease-resistant and economically more valuable.
The SUV4-20H Histone Methyltransferases in Health and Disease
Authors: Gabellini Davide , Pedrotti Simona
Published: April 2022

The post-translational modification of histone tails is a dynamic process that provides chromatin with high plasticity. Histone modifications occur through the recruitment of nonhistone proteins to chromatin and have the potential to influence fundamental biological processes. Many recent studies have been directed at understanding the role of methylated lysine 20 of histone H4 (H4K20) in physiological and pathological processes. In this review, we will focus on the function and regulation of the histone methyltransferases SUV4-20H1 and SUV4-20H2, which catalyze the di- and tri-methylation of H4K20 at H4K20me2 and H4K20me3, respectively. We will highlight recent studies that have elucidated the functions of these enzymes in various biological processes, including DNA repair, cell cycle regulation, and DNA replication. We will also provide an overview of the pathological conditions associated with H4K20me2/3 misregulation as a result of mutations or the aberrant expression of SUV4-20H1 or SUV4-20H2. Finally, we will critically analyze the data supporting these functions and outline questions for future research.
Induction of Brain Insulin Resistance and Alzheimer’s Molecular Changes by Western Diet
Authors: Mietelska-Porowska Anna , Domańska Justyna , Want Andrew , Więckowska-Gacek Angelika , Chutorański Dominik , Koperski Maciej , Wojda Urszula
Published: April 2022

The term Western diet (WD) describes the consumption of large amounts of highly processed foods, rich in simple sugars and saturated fats. Long-term WD feeding leads to insulin resistance, postulated as a risk factor for Alzheimer’s disease (AD). AD is the main cause of progressive dementia characterized by the deposition of amyloid-β (Aβ) plaques and neurofibrillary tangles consisting of the hyperphosphorylated tau (p-Tau) protein in the brain, starting from the entorhinal cortex and the hippocampus. In this study, we report that WD-derived impairment in insulin signaling induces tau and Aβ brain pathology in wild-type C57BL/6 mice, and that the entorhinal cortex is more sensitive than the hippocampus to the impairment of brain insulin signaling. In the brain areas developing WD-induced insulin resistance, we observed changes in p-Tau(Thr231) localization in neuronal subcellular compartments, indicating progressive tauopathy, and a decrease in amyloid precursor protein levels correlating with the appearance of Aβ peptides. These results suggest that WD promotes the development of AD and may be considered not only a risk factor, but also a modifiable trigger of AD.
Treating High COD Dyeing Wastewater via a Regenerative Sorption-Oxidation Process Using a Nano-Pored Activated Carbon
Authors: Ou Shih-Fu , Yang Dun-Sheng , Liao Jia-Wei , Chen Shyi-Tien
Published: April 2022

Nowadays, the structural complexity of dyes used in the textile industry and the widely adopted water-saving strategy in the dyeing processes often fail plants’ biological wastewater treatment units due to chemical oxygen demand (COD) overload. To alleviate this problems, this study investigated a regenerable adsorption–oxidation process to treat dyeing wastewater with COD around 10,000 mg/dm3 using a highly nano-pored activated carbon (AC) as a COD adsorbent, followed by its regeneration using hydrogen peroxide as an oxidizing reagent. In addition to studying AC’s COD adsorption and oxidation performance, its operational treatment conditions in terms of temperature and pH were assessed. The results firstly demonstrated that about 50–60% of the COD was consistently adsorbed during the repeated adsorption operation before reaching AC’s maximum adsorption capacity (qmax) of 0.165 g-COD/g-AC. The optimal pH and temperature during adsorption were 4.7 and 25 °C, respectively. Secondly, AC regeneration was accomplished by using an initial peroxide concentration of 2.5% (by wt %) and EDTA-Fe of 2.12 mmole/dm3. The reuse of the regenerated ACs was doable. Surprisingly, after the first AC regeneration, the COD adsorption capacity of the regenerated AC even increased by ~7% with respect to the virgin AC. Thirdly, the results of a five-consecutive adsorption–regeneration operation showed that a total of 0.3625 g COD was removed by the 5 g AC used, which was equivalent to an adsorption capacity (q) of 0.0725 (= 0.3625/5) g-COD/g-AC during each adsorption stage. Based on the obtained results, a regenerable COD adsorption–oxidation process using a nano-pored AC to treat the high-textile-COD wastewater looks promising. Thus, a conceptual treatment unit was proposed, and its potential benefits and limitations were addressed.
On the Base Composition of Transposable Elements
Authors: Boissinot Stéphane
Published: April 2022

Transposable elements exhibit a base composition that is often different from the genomic average and from hosts’ genes. The most common compositional bias is towards Adenosine and Thymine, although this bias is not universal, and elements with drastically different base composition can coexist within the same genome. The AT-richness of transposable elements is apparently maladaptive because it results in poor transcription and sub-optimal translation of proteins encoded by the elements. The cause(s) of this unusual base composition remain unclear and have yet to be investigated. Here, I review what is known about the nucleotide content of transposable elements and how this content can affect the genome of their host as well as their own replication. The compositional bias of transposable elements could result from several non-exclusive processes including horizontal transfer, mutational bias, and selection. It appears that mutation alone cannot explain the high AT-content of transposons and that selection plays a major role in the evolution of the compositional bias. The reason why selection would favor a maladaptive nucleotide content remains however unexplained and is an area of investigation that clearly deserves attention.
The Regulatory Circuit Underlying Downregulation of a Type III Secretion System in Yersinia enterocolitica by Transcription Factor OmpR
Authors: Nieckarz Marta , Jaworska Karolina , Raczkowska Adrianna , Brzostek Katarzyna
Published: April 2022

In a previous study, differential proteomic analysis was used to identify membrane proteins of the human enteropathogen Yersinia enterocolitica, whose levels are influenced by OmpR, the transcriptional regulator in the two-component EnvZ/OmpR system. Interestingly, this analysis demonstrated that at 37 °C, OmpR negatively affects the level of over a dozen Ysc-Yop proteins, which constitute a type III secretion system (T3SS) that is essential for the pathogenicity of Y. enterocolitica. Here, we focused our analysis on the role of OmpR in the expression and secretion of Yops (translocators and effectors). Western blotting with anti-Yops antiserum and specific anti-YopD, -YopE and -YopH antibodies, confirmed that the production of Yops is down-regulated by OmpR with the greatest negative effect on YopD. The RT-qPCR analysis demonstrated that, while OmpR had a negligible effect on the activity of regulatory genes virF and yscM1, it highly repressed the expression of yopD. OmpR was found to bind to the promoter of the lcrGVsycD-yopBD operon, suggesting a direct regulatory effect. In addition, we demonstrated that the negative regulatory influence of OmpR on the Ysc-Yop T3SS correlated with its positive role in the expression of flhDC, the master regulator of the flagellar-associated T3SS.
Hydrothermal Synthesis of β-Nb2ZnO6 Nanoparticles for Photocatalytic Degradation of Methyl Orange and Cytotoxicity Study
Authors: Almofty Sarah Ameen , Nawaz Muhammad , Qureshi Faiza , Al-Mutairi Rayanah
Published: April 2022

β-Nb2ZnO6 nanoparticles were synthesized by a hydrothermal process and calcined at two temperatures, 500 °C and 700 °C, and assigned as A and B, respectively. X-ray diffraction, together with transmission electron microscopy, revealed that the β-Nb2ZnO6 nanoparticles calcined at 700 °C (B) were more crystalline than the β-Nb2ZnO6 calcined at 500 °C (A) with both types of nanoparticles having an average size of approximately 100 nm. The physiochemical, photocatalytic, and cytotoxic activities of both types of β-Nb2ZnO6 nanoparticles (A and B) were examined. Interestingly, the photodegradation of methyl orange, used as a standard for environmental pollutants, was faster in the presence of the β-Nb2ZnO6 nanoparticles calcined at 500 °C (A) than in the presence of those calcined at 700 °C (B). Moreover, the cytotoxicity was evaluated against different types of cancer cells and the results indicated that both types of β-Nb2ZnO6 nanoparticles (A and B) exhibited high cytotoxicity against MCF-7 and HCT116 cells but low cytotoxicity against HeLa cells after 24 and 48 h of treatment. Overall, both products expressed similar EC50 values on tested cell lines and high cytotoxicity after 72 h of treatment. As a photocatalyst, β-Nb2ZnO6 nanoparticles (A) could be utilized in different applications including the purification of the environment and water from specific pollutants. Further biological studies are required to determine the other potential impacts of utilizing β-Nb2ZnO6 nanoparticles in the biomedical application field.
Regulation of Poly-E Motif Flexibility by pH, Ca2+ and the PPAK Motif
Authors: Dassanayake Mudiyanselage Sudarshi Premawardhana , Gage Matthew J.
Published: April 2022

The disordered PEVK region of titin contains two main structural motifs: PPAK and poly-E. The distribution of these motifs in the PEVK region contributes to the elastic properties of this region, but the specific mechanism of how these motifs work together remains unclear. Previous work from our lab has demonstrated that 28-amino acid peptides of the poly-E motif are sensitive to shifts in pH, becoming more flexible as the pH decreases. We extend this work to longer poly-E constructs, including constructs containing PPAK motifs. Our results demonstrate that longer poly-E motifs have a much larger range of pH sensitivity and that the inclusion of the PPAK motif reduces this sensitivity. We also demonstrate that binding calcium can increase the conformational flexibility of the poly-E motif, though the PPAK motif can block this calcium-dependent change. The data presented here suggest a model where PPAK and calcium can alter the stiffness of the poly-E motif by modulating the degree of charge repulsion in the glutamate clusters.
Targeting Cell Death Mechanism Specifically in Triple Negative Breast Cancer Cell Lines
Authors: Pruteanu Lavinia-Lorena , Braicu Cornelia , Módos Dezső , Jurj Maria-Ancuţa , Raduly Lajos-Zsolt , Zănoagă Oana , Magdo Lorand , Cojocneanu Roxana , Paşca Sergiu , Moldovan Cristian , Moldovan Alin Iulian , Ţigu Adrian Bogdan , Gurzău Eugen , Jäntschi Lorentz , Bender Andreas , Berindan-Neagoe Ioana
Published: April 2022

Triple negative breast cancer (TNBC) is currently associated with a lack of treatment options. Arsenic derivatives have shown antitumoral activity both in vitro and in vivo; however, their mode of action is not completely understood. In this work we evaluate the response to arsenate of the double positive MCF-7 breast cancer cell line as well as of two different TNBC cell lines, Hs578T and MDA-MB-231. Multimodal experiments were conducted to this end, using functional assays and microarrays. Arsenate was found to induce cytoskeletal alteration, autophagy and apoptosis in TNBC cells, and moderate effects in MCF-7 cells. Gene expression analysis showed that the TNBC cell lines’ response to arsenate was more prominent in the G2M checkpoint, autophagy and apoptosis compared to the Human Mammary Epithelial Cells (HMEC) and MCF-7 cell lines. We confirmed the downregulation of anti-apoptotic genes (MCL1, BCL2, TGFβ1 and CCND1) by qRT-PCR, and on the protein level, for TGFβ2, by ELISA. Insight into the mode of action of arsenate in TNBC cell lines it is provided, and we concluded that TNBC and non-TNBC cell lines reacted differently to arsenate treatment in this particular experimental setup. We suggest the future research of arsenate as a treatment strategy against TNBC.
GenoType CM Direct® and VisionArray Myco® for the Rapid Identification of Mycobacteria from Clinical Specimens
Authors: Schildhaus Hans-Ulrich , Steindor Mathis , Kölsch Bernd , Herold Thomas , Buer Jan , Kehrmann Jan
Published: April 2022

M. tuberculosis is the single infectious agent responsible for most deaths worldwide outside of pandemics. Diseases due to non-tuberculous mycobacteria (NTM) are increasing in many regions of the world. The two molecular assays GenoType CM direct® (GTCMd) (Bruker, Billerica, MA, USA) and VisionArray Myco® (VAM) (ZytoVision, Bremerhaven, Germany) are based on the DNA/DNA hybridization technique, and allow for the identification of tuberculous and the most clinically relevant non-tuberculous mycobacterial species from clinical specimens. We evaluated the performance of both assays for the identification of mycobacteria from 65 clinical specimens of 65 patients and compared it with the results of conventional culture. Based on conventional culture that recovered 37 mycobacterial isolates including 11 tuberculous and 26 NTM isolates, sensitivity, specificity, positive predictive value and negative predictive value were 89.2%, 81.5%, 86.8% and 84.6% for GTCMd and 73.0%, 96.3%, 96.4% and 72.2% for VAM. Additionally, GTCMd identified mycobacteria from five and VAM from one culture-negative sample. Both assays identified a mycobacterium in one sample overgrown by other microorganisms. Two M. abscessus subsp. abscessus isolates grown from culture were identified as M. chelonae by GTCMd assay. In conclusion, both assays improve the rapid identification of mycobacteria directly from clinical specimens.
The Impact of Resistance Training Program on Static Balance in Multiple Sclerosis Population: A Randomized Controlled Trial Study
Authors: Andreu-Caravaca Luis , Ramos-Campo Domingo J. , Manonelles Pedro , Chung Linda H. , Ramallo Salvador , Rubio-Arias Jacobo Á.
Published: April 2022

Background: Multiple sclerosis (MS) is a neurological disease that affects balance. Among the non-pharmacological strategies to improve this variable, physical exercise is one of the most widely used. However, the benefits of some types of training, such as resistance training, on static balance in this population are still unclear. This study aims to analyze the effects of a resistance training (RT) intervention on balance in people with MS. Methods: Thirty people with MS were randomized to either an experimental (n = 18) or a control (n = 12) group. The RT group performed 10 weeks of lower limb resistance training with a concentric phase at maximum velocity. Static balance was measured before and after intervention. Results: No significant group × time interaction effects were found (ANOVA test) in any of the variables at the end of the intervention. No intragroup differences were found before or after the intervention in the balance variables. Conclusions: Resistance training with a concentric phase at maximum velocity showed no impact on balance in our sample. Future studies should examine programs of longer duration or combined with other types of training, such as balance training, with the aim of obtaining improvements in this variable in people with MS.
Three Clinical Clusters Identified through Hierarchical Cluster Analysis Using Initial Laboratory Findings in Korean Patients with Systemic Lupus Erythematosus
Authors: Jung Ju-Yang , Lee Hyun-Young , Lee Eunyoung , Kim Hyoun-Ah , Yoon Dukyong , Suh Chang-Hee
Published: April 2022

Systemic lupus erythematosus (SLE) is a heterogeneous disorder with diverse clinical manifestations. This study classified patients by combining laboratory values at SLE diagnosis via hierarchical cluster analysis. Linear discriminant analysis was performed to construct a model for predicting clusters. Cluster analysis using data from 389 patients with SLE yielded three clusters with different laboratory characteristics. Cluster 1 had the youngest age at diagnosis and showed significantly lower lymphocyte and platelet counts and hemoglobin and complement levels and the highest erythrocyte sedimentation rate (ESR) and anti-double-stranded DNA (dsDNA) antibody level. Cluster 2 showed higher white blood cell (WBC), lymphocyte, and platelet counts and lower ESR and anti-dsDNA antibody level. Cluster 3 showed the highest anti-nuclear antibody titer and lower WBC and lymphocyte counts. Within approximately 171 months, Cluster 1 showed higher SLE Disease Activity Index scores and number of cumulative manifestations, including malar rash, alopecia, arthritis, and renal disease, than did Clusters 2 and 3. However, the damage index and mortality rate did not differ significantly between them. In conclusion, the cluster analysis using the initial laboratory findings of the patients with SLE identified three clusters. While disease activities, organ involvements, and management patterns differed between the clusters, damages and mortalities did not.
References Values of Soluble α-Klotho Serum Levels Using an Enzyme-Linked Immunosorbent Assay in Healthy Adults Aged 18–85 Years
Authors: Espuch-Oliver Andrea , Vázquez-Lorente Héctor , Jurado-Fasoli Lucas , de Haro-Muñoz Tomás , Díaz-Alberola Irene , López-Velez María del Señor , de Haro-Romero Teresa , Castillo Manuel J. , Amaro-Gahete Francisco J.
Published: April 2022

α-Klotho protein is a powerful predictor of the aging process and lifespan. Although lowered circulating soluble α-Klotho levels have been observed in aged non-healthy individuals, no specific reference values across a wide range of ages and sex using an enzyme-linked immunosorbent assay (ELISA) are available for larger cohorts of healthy individuals. The present analytical cross-sectional study was aimed to establish the reference values of soluble α-Klotho serum levels in healthy adults by age and sex groups. A total of 346 (59% women) healthy individuals aged from 18 to 85 years were recruited. Subjects were divided by sex and age as: (i) young (18–34.9 years), (ii) middle-aged (35–54.9 years), and (iii) senior (55–85 years) individuals. The soluble α-Klotho levels were measured in serum using ELISA. Senior adults were the age-group that presented the lowest soluble α-Klotho serum levels (p < 0.01), with age showing a negative association with soluble α-Klotho serum levels (p < 0.001). No differences between sexes were observed. Therefore, soluble α-Klotho levels were especially decreased—regardless of sex—in our cohort of healthy individuals because of the physiological decline derived from the aging process. We recommend routine assessments of soluble α-Klotho levels using ELISA as a simple and cheap detectable marker of aging that improves quality of life in the elderly.
Mid-Term Mortality in Older Anemic Patients with Type 2 Myocardial Infarction: Does Blood Transfusion sImprove Prognosis?
Authors: Hacquin Arthur , Putot Alain , Chague Frederic , Manckoundia Patrick , Cottin Yves , Zeller Marianne
Published: April 2022

(1) Anemia often predisposes older patients to type 2 myocardial infarction (T2MI). However, the management of this frequent association remains uncertain. We aimed to evaluate the impact of red blood cell transfusion during the acute phase of T2MI in older anemic inpatients. (2) Methods and results: We performed a retrospective study using a French regional database. One hundred and seventy-eight patients aged 65 years or older, presenting with a T2MI and anemia, were selected. Patients were split into two groups: one that received a red blood cell transfusion (≥1 red blood cell unit) and one that did not. A propensity score was built to adjust for potential confounders, and the association between transfusion and 30-day mortality was evaluated with an inverse propensity score weighted Cox model. Transfusion was not associated with 30-day all-cause mortality (propensity score weighted hazard ratio (HR) 1.59 (0.55–4.56), p = 0.38). However, 1-year all-cause mortality was significantly higher in the transfusion group (propensity score weighted HR 2.47 (1.22–4.97), p = 0.011). (3) Conclusion: Our findings in older adults with anemia suggest that blood transfusion in the acute phase of T2MI could not be associated with improved short-term prognosis. Prospective studies are urgently needed to assess the impact of transfusion on longer-term prognosis.
Significance of Serum-Plasma Leptin Profile during Pregnancy in Gestational Diabetes Mellitus: A Systematic Review and Meta-Analysis
Authors: Roca-Rodríguez María del Mar , Ramos-García Pablo , López-Tinoco Cristina , Aguilar-Diosdado Manuel
Published: April 2022

Gestational diabetes mellitus (GDM) represents a stage of subclinical inflammation and a risk factor for subsequent future type 2 diabetes and cardiovascular disease development. Leptin has been related with vascular and metabolic changes in GDM with heterogeneous and contradictory results with respect to their possible involvement in maternal, perinatal, and future complications. Our objective is to evaluate current evidence on the role of leptin in maternal and perinatal complications in women with GDM. PubMed, Embase, Web of Science, and Scopus databases were searched. We evaluated the studies’ quality using the Newcastle-Ottawa scale. Meta-analyses were conducted, and heterogeneity and publication bias were examined. Thirty-nine relevant studies were finally included, recruiting 2255 GDM and 3846 control pregnant women. Leptin levels were significantly higher in GDM participants than in controls (SMD = 0.57, 95%CI = 0.19 to 0.94; p < 0.001). Subgroup meta-analysis did not evidence significant differences in leptin in the different trimesters of pregnancy. Meta-regression showed a positive significant relationship for HOMA in the GDM group (p = 0.05). According to these results, it seems that high levels of leptin can be used as predictive markers in GDM.
Pandemic Preparedness: The Importance of Adequate Immune Fitness
Authors: Kiani Pantea , Balikji Jessica , Kraneveld Aletta D. , Garssen Johan , Bruce Gillian , Verster Joris C.
Published: April 2022

Pandemic preparedness is an important issue in relation to future pandemics. The two studies described here aimed to identify factors predicting the presence and severity of coronavirus disease 2019 (COVID-19) symptoms. The CLOFIT study comprised an online survey among the Dutch population (n = 1415). Perceived immune fitness before the pandemic (2019) and during the first lockdown period (15 March–11 May 2020) and the number and severity of COVID-19 symptoms were assessed. The COTEST study, conducted between December 2020 and June 2021, replicated the CLOFIT study in n = 925 participants who were tested for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Dutch commercial test locations. The CLOFIT study revealed that immune fitness before the pandemic was the greatest predictor of the number and severity of COVID-19 symptoms (20.1% and 19.8%, respectively). Other significant predictors included immune fitness during the lockdown (5.5% and 7.1%, respectively), and having underlying diseases (0.4% and 0.5%, respectively). In the COTEST study, for those who tested positive for SARS-CoV-2, immune fitness before the pandemic was the single predictor of the number (27.2%) and severity (33.1%) of COVID-19 symptoms during the pandemic. In conclusion, for those who tested positive for SARS-CoV-2, immune fitness before the pandemic was the strongest predictor of the number and severity of COVID-19 symptoms during the pandemic. Therefore, the development of strategies to maintain an adequate immune fitness must be regarded as an essential component of pandemic preparedness.
3D Printing of Calcium Phosphate/Calcium Sulfate with Alginate/Cellulose-Based Scaffolds for Bone Regeneration: Multilayer Fabrication and Characterization
Authors: Wattanaanek Nattanan , Suttapreyasri Srisurang , Samruajbenjakun Bancha
Published: April 2022

Congenital abnormalities, trauma, and disease result in significant demands for bone replacement in the craniofacial region and across the body. Tetra-compositions of organic and inorganic scaffolds could provide advantages for bone regeneration. This research aimed to fabricate and characterize amorphous calcium phosphate (ACP)/calcium sulfate hemihydrate (CSH) with alginate/cellulose composite scaffolds using 3D printing. Alginate/cellulose gels were incorporated with 0%, 13%, 15%, 18%, 20%, and 23% ACP/CSH using the one-pot process to improve morphological, physiochemical, mechanical, and biological properties. SEM displayed multi-staggered filament layers with mean pore sizes from 298 to 377 μm. A profilometer revealed mean surface roughness values from 43 to 62 nm that were not statistically different. A universal test machine displayed the highest compressive strength and modulus with a statistical significance in the 20% ACP/CSH group. FTIR spectroscopy showed peaks in carbonate, phosphate, and sulfate groups that increased as more ACP/CSH was added. Zero percent of ACP/CSH showed the highest swelling and lowest remaining weight after degradation. The 23% ACP/CSH groups cracked after 60 days. In vitro biocompatibility testing used the mouse osteoblast-like cell line MC3T3-E1. The 18% and 20% ACP/CSH groups showed the highest cell proliferation on days five and seven. The 20% ACP/CSH was most suitable for bone cell regeneration.
Infrared and Visible Image Fusion Methods for Unmanned Surface Vessels with Marine Applications
Authors: Zhang Renran , Su Yumin , Li Yifan , Zhang Lei , Feng Jiaxiang
Published: April 2022

Infrared and visible image fusion is a very effective way to solve the degradation of sea images for unmanned surface vessels (USVs). Fused images with more clarity and information are useful for the visual system of USVs, especially in harsh marine environments. In this work, three novel fusion strategies based on adaptive weight, cross bilateral filtering, and guided filtering are proposed to fuse the feature maps that are extracted from source images. First, the infrared and visible cameras equipped on the USV are calibrated using a self-designed calibration board. Then, pairs of images containing water scenes are aligned and used as experimental data. Finally, each proposed strategy is inserted into the neural network as a fusion layer to verify the improvements in quality of water surface images. Compared to existing methods, the proposed method based on adaptive weight provides a higher spatial resolution and, in most cases, less spectral distortion. The experimental results show that the visual quality of fused images obtained based on an adaptive weight strategy is superior compared to other strategies, while also providing an acceptable computational load.
A Straightforward Approach to Synthesize 7-Aminocephalosporanic Acid In Vivo in the Cephalosporin C Producer Acremonium chrysogenum
Authors: Lin Xuemei , Lambertz Jan , Dahlmann Tim A. , Nowaczyk Marc M. , König Burghard , Kück Ulrich
Published: April 2022

The pharmaceutical industry has developed various highly effective semi-synthetic cephalosporins, which are generated by modifying the side chains of the core molecule 7-aminocephalosporanic acid (7-ACA). In industrial productions, the 7-ACA nucleus is obtained in vitro from cephalosporin C (CPC) by chemical or enzymatic processes, which are waste intensive and associated with high production costs. Here, we used a transgenic in vivo approach to express bacterial genes for cephalosporin C acylase (CCA) in the CPC producer Acremonium chrysogenum. Western blot and mass spectrometry analyses verified that the heterologous enzymes are processed into α- and β-subunits in the fungal cell. Extensive HPLC analysis detected substrates and products of CCAs in both fungal mycelia and culture supernatants, with the highest amount of 7-ACA found in the latter. Using different incubation times, temperatures, and pH values, we explored the optimal conditions for the active bacterial acylase to convert CPC into 7-ACA in the culture supernatant. We calculated that the best transgenic fungal strains exhibit a one-step conversion rate of the bacterial acylase of 30%. Our findings can be considered a remarkable contribution to supporting future pharmaceutical manufacturing processes with reduced production costs.
The Need for Oral Hygiene Care and Periodontal Status among Hospitalized Gastric Cancer Patients
Authors: Nicolae Flavia Mirela , Bennardo Francesco , Barone Selene , Șurlin Petra , Gheorghe Dorin Nicolae , Burtea Daniela , Pătrascu Ștefan , Râmboiu Sandu , Radu Adrian Petru , Ungureanu Bogdan Silviu , Turcu-Știolica Adina , Didilescu Andreea Cristiana , Strâmbu Victor Dan Eugen , Șurlin Valeriu Marin , Gheonea Dan Ionuț
Published: April 2022

Poor oral hygiene leads to the accumulation of dental plaque, thus contributing to the initiation of periodontal disease (PD). Local infections can lead to systemic inflammatory responses, which are essential mediators for the evolution of systemic conditions or cancer tumorigenesis. Often, patients hospitalized with life-threatening and incapacitating disorders such as gastric cancer (GC) might lose interest in keeping their mouth healthy. This study evaluates oral hygiene, periodontal status, and the need for oral care and medical personnel to assist in achieving it in patients hospitalized with GC. This study was carried out on 25 patients with a diagnosis of GC, divided into two groups (GP—14 patients from the Gastroenterology Department, and SP—11 patients from the 1st Department of Surgery). Patients were examined on the day of admission (T0), the day of the medical procedure of endoscopy or surgery (T1), and the day of discharge (T2), recording the number of absent teeth, dental plaque (PI), bleeding on probing (BOP), probing depths (PPD), frequency of toothbrushing, and if the oral hygiene had been self-performed or assisted. Data were subjected to statistical analysis. Our results showed that, in both the GP and the SP group, there were strong and statistically significant correlations between PI and BOP measured on the last day of hospitalization and the period of hospitalization after the medical procedure. Longer hospital stays and the presence of surgery were risk factors for changing toothbrushing frequency. Results also highlight the need for a dentist to diagnose and eventually treat periodontal disease before and after hospitalization, and for a trained nurse who should help take care of the patient’s oral hygiene during hospitalization.
Simulation-Based Business Valuation: Methodical Implementation in the Valuation Practice
Authors: Ernst Dietmar
Published: April 2022

The simulation-based company valuation values a company on the basis of the risks actually present in the company without having to derive them from the capital market data. The simulation-based company valuation takes into account the market imperfections, such as the probability of insolvency or the lack of diversification, and fulfils the legal requirements and auditing standards for a company valuation. The simulation-based company valuation is an alternative to the CAPM-based company valuation, which, under the assumption of perfect capital markets, derives the risks through capital market comparisons. A simulation-based business valuation has many advantages and is particularly suitable for valuing medium-sized companies, start-ups, companies in a crisis, and for integrating country-specific risks into business valuations. Due to the internationally widespread use of the CAPM, a simulation-based company valuation is still rarely used in practice. This article shows which valuation formulas are necessary for the application of a simulation-based company valuation. These are used for both the certainty equivalent method and for the risk premium method. In a concrete and valuation example, the simulation-based business planning and company valuation is carried out, and the derived valuation formulas are applied in a way that allows a transfer to concrete valuation cases in practice. It is shown that the certainty equivalent method and the risk premium method lead to identical company values.
How Is Construction Land Transition Related to Rural Transformation? Evidence from a Plain County in China Based on the Grey Correlation Model
Authors: Shan Bowen , Liu Jian , Liu Yaqiu , Wang Huanhuan , Wang Ailing
Published: April 2022

Under the background of urban-rural integration, the frequent flow of urban and rural elements has promoted the close connection between rural transformation (RT) and construction land transition (CLT). How is CLT related to RT? By taking the plain area Linqing County in China as the study area, basing the study on the RT and CLT coupling framework of relevance theory, building an RT and CLT evaluation index system, using the multi-factor evaluation method for the evaluation of RT and CLT from 2010 to 2018, and using the grey correlation model to measure the RT and CLT coupling relationship, the results showed that the level of RT was from 0.04 to 97.42, and the level of CLT was from 14.89 to 82.47, showing the trends of gradual increase and fluctuating increase, respectively. Taking 2013 and 2016 as the time point, RT could be divided into the initial stage, growth stage I, and growth stage II, corresponding to the initial stage, high growth stage, and stable development stage of CLT. The coupling degree between the two was in the range of 0.6–0.8 and was in the stage of a medium to high coupling degree. The correlation degree between the subsystem of RT and CLT was over 0.65. In the subsystem of CLT, the correlation degrees between quantitative structure transition and RT and efficiency transition and RT were both lower than 0.65, which were relatively low. Controlling the scale of construction land and taking efficiency transition are the effective methods to guide the deep exploration of potential and are the inevitable way to strengthen the relationship between CLT and RT and promote urban-rural integration development.
Structural Change in Relative Clauses and the Autonomy of Heritage Grammars
Authors: Frasson Alberto
Published: April 2022

This paper addresses the question of structural change in relative clauses in heritage speakers of two varieties of Venetan, a northern Italo-Romance language. It will be shown that appositive and restrictive relative clauses are not structurally distinguished in Brazilian Venetan, while they display different structural properties in Italian Venetan. It will be proposed that the phenomenon described in the paper does not depend on transfer from another language and it is not exclusively a matter of processing. The approach presented here aims to account for structural change in syntactic terms, without resorting to extra-linguistic factors. Heritage grammars are autonomous systems and follow predictable paths of language variation, as such, variation may take place at an interface level and at a syntactic level alike. This does not exclude possible influences from the dominant language, which, however, do not need to be taken as the only triggers of change.
Relationship between the Levels of lncRNA H19 in Plasma and Different Adipose Tissue Depots with Patients’ Response to Bariatric Surgery
Authors: Artemyeva Marina S. , Vasileva Ludmila B. , Ma Yi , Kondratov Kirill A. , Fedorov Anton V. , Kostareva Anna A. , Lapshina Sofia E. , Anopova Anna D. , Likhonosov Nikolai P. , Neymark Alexander E. , Babenko Alina Yu. , Shlyakhto Evgeny V.
Published: April 2022

Bariatric surgery represents a widespread approach to treating morbid obesity. The search for biomarkers to identify patients to whom this type of treatment will be most effective is needed. Our aim was to characterize the relationship of levels of lncRNA H19 in plasma and different adipose tissue depots with patients’ response to bariatric surgery. The study includes control subjects, patients with obesity and patients with obesity accompanied by impaired carbohydrate metabolism (ICM). Quantitative analysis of lncRNA H19 levels has been performed using qPCR in plasma and subcutaneous (SAT) and visceral adipose tissue (VAT). Patients with obesity without ICM have higher levels of lncRNA H19 in VAT compared to SAT, and higher levels of lncRNA H19 in SAT compared to SAT of control individuals. One year after the intervention, levels of lncRNA H19 decreased in SAT of patients with obesity without ICM. The preoperative level of lncRNA H19 in VAT demonstrates a positive correlation with excess weight loss and a negative correlation with initial BMI. In conclusion, ICM affects expression of lncRNA H19 in SAT of patients with obesity. The preoperative level of lncRNA H19 in VAT can be used to predict excess weight loss in patients with obesity after bariatric surgery.
Brunneosporopsis yunnanensis gen. et sp. nov. and Allocryptovalsa xishuangbanica sp. nov., New Terrestrial Sordariomycetes from Southwest China
Authors: Maharachchikumbura Sajeewa S. N. , Wanasinghe Dhanushka N. , Elgorban Abdallah M. , Al-Rejaie Salim S. , Kazerooni Elham A. , Cheewangkoon Ratchadawan
Published: April 2022

Three fungal taxa were collected on dead branches of wood during fieldwork in Sichuan and Yunnan Provinces, China. The new generic name Brunneosporopsis gen. nov. and species B. yunnanensis sp. nov. are introduced for a novel taxon characterized by globose to subglobose and dark olivacous-brown conidia. Phylogenetic analyses based on combined LSU, SSU and tef1-α loci strongly support the monophyly of this taxon and place it in the subclass Diaporthomycetidae. It could not be assigned to any currently recognized families in the subclass and was, therefore, placed in the Diaporthomycetidae genera incertae sedis. A second taxon represents a new species in Allocryptovalsa based on an analysis of the sequence datasets of ITS and btub loci of the novel, brown-spored sexual morphic species. This taxon is described here as A. xishuangbanica sp. nov. An interesting hypocrealean fungus producing synnemata, Stilbocrea gracilipes, was collected from dead wood of an unknown host from Sichuan Province and is reported here, with asexual morph from both the host and culture as well as LSU, ITS, tef1-α, rpb2 and rpb1 sequence data.
Structural Balance under Weight Evolution of Dynamic Signed Network
Authors: Li Zhenpeng , Ma Ling , Chi Simin , Qian Xu
Published: April 2022

The mutual feedback mechanism between system structure and system function is the ‘hot spot’ of a complex network. In this paper, we propose an opinions–edges co-evolution model on a weighted signed network. By incorporating different social factors, five evolutionary scenarios were simulated to investigate the feedback effects. The scenarios included the variations of edges and signed weights and the variations of the proportions of positive and negative opinions. The level of balance achieved depends on the connection weight and the distribution of negative edges/opinions on the signed graph. This paper sheds light on the analysis of constraints and opportunities of social and cognitive processes, helping us understand the real-world opinions polarization process in depth. For example, the results serve as a confirmation of the imperfect balance theory, i.e., even if the system evolves to a stable state, the signed network still cannot achieve perfect structural balance.
Asymptotic Behavior of a Surface Implicitly Defined
Authors: Campo-Montalvo Elena , Fernández de Sevilla Marián , Pérez-Díaz Sonia
Published: April 2022

In this paper, we introduce the notion of infinity branches and approaching surfaces. We obtain an algorithm that compares the behavior at the infinity of two given algebraic surfaces that are defined by an irreducible polynomial. Furthermore, we show that if two surfaces have the same asymptotic behavior, the Hausdorff distance between them is finite. All these concepts are new and represent a great advance for the study of surfaces and their applications.
Extended Lymphadenectomy for Proximal Transverse Colon Cancer: Is There a Place for Standardization?
Authors: Popescu Răzvan Cătălin , Botea Florin , Dumitru Eugen , Mazilu Laura , Micu Luminița Gențiana , Tocia Cristina , Dumitru Andrei , Croitoru Adina , Leopa Nicoleta
Published: April 2022

Background and Objectives: Complete mesocolon excision and high vascular ligation have become a standard procedure in the treatment of colon cancer. The transverse colon has certain embryological and anatomical particularities which require special attention in case of oncological surgeries. Proximal transverse colon cancer (TCC) can metastasize to the lymph nodes in the gastrocolic ligament. The aim of this study is to assess the tumor involvement of these lymph nodes and to determine the applicability of gastrocolic ligament lymph nodes dissection as the standard approach for proximal transverse colon cancer. Materials and Methods: this study analyzes the cases of patients admitted to the Surgery Department, diagnosed with proximal transverse colon cancer, with tumor invasion ≥ T2 and for which complete mesocolon excision with high vascular ligation and lymphadenectomy of the gastrocolic ligament (No. 204, 206, 214v) were performed. Results: A total of 43 cases operated during 2015–2020 were included in the study. The median total number of retrieved central lymph nodes was 23 (range, 12–38), that had tumor involvement in 22 cases (51.2%). Gastrocolic ligament tumor involvement was found in 5 cases (11.6%). The median operation time was 180 min, while the median blood loss was 115 mL (range 0–210). The median time of hospitalization was 6 days (range, 5–11). Grade IIIA in the Clavien-Dindo classification was noticed in 3 patients, with no mortality. Upon Kaplan–Meier analysis, tumors > T3 (p < 0.016) and lymph node ratio < 0.05 (p < 0.025) were statistically significant. Conclusions: lymph node dissection of the gastrocolic ligament in patients with advanced proximal transverse colon cancer may improve the oncological outcome in T3/T4 tumors, and therefore standardization could be feasible
Life Cycle Assessment of Hybrid Nanofiltration Desalination Plants in the Persian Gulf
Authors: Bordbar Benyamin , Khosravi Arash , Ahmadi Orkomi Ali , Peydayesh Mohammad
Published: April 2022

Although emerging desalination technologies such as hybrid technologies are required to tackle water scarcity, the impacts of their application on the environment, resources, and human health, as prominent pillars of sustainability, should be evaluated in parallel. In the present study, the environmental footprint of five desalination plants, including multi-stage flash (MSF), hybrid reverse osmosis (RO)–MSF, hybrid nanofiltration (NF)–MSF, RO, and hybrid NF–RO, in the Persian Gulf region, have been analyzed using life cycle assessment (LCA) as an effective tool for policy making and opting sustainable technologies. The comparison was based on the impacts on climate change, ozone depletion, fossil depletion, human toxicity, and marine eutrophication. The LCA results revealed the superiority of the hybrid NF–RO plant in having the lowest environmental impact, although the RO process produces more desalinated water at the same feed and input flow rates. The hybrid NF–RO system achieves 1.74 kg CO2 equivalent, 1.24 × 10−7 kg CFC-11 equivalent, 1.28 × 10−4 kg nitrogenous compounds, 0.16 kg 1,4-DB equivalent, and 0.56 kg oil equivalent in the mentioned impact indicators, which are 7.9 to 22.2% lower than the single-pass RO case. Furthermore, the sensitivity analysis showed the reliability of the results, which helps to provide an insight into the life cycle impacts of the desalination plants.
A Smart Digital Twin to Stabilize Return Sand Temperature without Using Coolers
Authors: Nieves Javier , Bravo Beñat , Sierra David-Ciro
Published: April 2022

In order to ensure the optimal state of recovered molding sand inside a foundry, it is necessary to avoid temperature peaks and to ensure optimal humidity conditions prior to reusing the sand. Sand that is too hot or without optimal moisture can cause production delays due to a long mixing process, excessive consumption of raw materials, or poor agglutination. To ensure a stable and optimal sand temperature, many foundries choose to incorporate coolers into their process, however, it is a solution that is not always viable, either due to their high cost or a lack of space within the facility. Another solution is to incorporate water sprinklers into the cooling drum which contribute by reducing the temperature of the castings and the sand, but these systems do not prevent temperature peaks from occurring. Therefore, here, we present a control methodology, based on a digital architecture that, governed by an intelligent digital twin allows us to know the real situation and the current rate of production, providing suggestions for water addition. The obtained system reduces the average temperature and its variation, as well as eliminates temperature peaks, giving a more controlled manufacturing process.