bluuebunny/crossref_metadata_2025 huggingface dataset:
The Prognostic Value of Deleted in Colorectal Cancer (DCC) Receptor and Serum Netrin-1 in Severe Traumatic Brain Injury
Authors: Zhang Yuanda , Zhang Qiao , Sun Lihua , Zhao Dongxu , Ruan Cijie , Zhou Jue , Tan Haoyuan , Bao Yinghui
Published: June 2022

Traumatic brain injury (TBI) is a common neurological disease. Netrin-1 and deleted in colorectal cancer (DCC) receptor are potential biomarkers associated with nerve regeneration and immune regulation. We aimed to investigate the ability of the DCC receptor and Netrin-1 to predict a high ICP level after operation in severe traumatic brain injury and their prognostic significance. This study is a prospective observational study. We selected 23 patients with traumatic brain injury who had undergone surgical operations as subjects. Immunohistochemical staining was performed on the contusion tissue that was removed by the operation to determine the expression of DCC receptor. At the same time, enzyme-linked immunosorbent assay (ELISA) kits were used to detect the serum Netrin-1 content. Determination of intracranial pressure (ICP) value was measured by intraventricular catheter. The Glasgow Outcome Scale (GOS) score at six months after trauma was defined as the main study endpoint. The results showed that serum Netrin-1 concentrations of patients in the critical TBI group (GCS 3–5 points) was significantly lower than that in the severe TBI group (GCS 6–8 points). The ICP peak and average mannitol consumption in the high Netrin-1 group were significantly lower than those in the low Netrin-1 group. DCC receptor-positive patients had a significantly lower ICP peak. There was no significant difference in six month-GOS scores between patients in the high and low Netrin-1 groups, while DCC receptor concentrations below 3.82 ng/mL predicted poor prognosis (GOS 1–3 points). In conclusion, the expression level of the DCC receptor can better evaluate the postoperative high ICP level and prognosis than the level of serum Netrin-1 in severe traumatic brain injury.
Cutaneous Adverse Reactions Associated with Monoclonal Antibodies Treatment in Multiple Sclerosis: Case Reports and Short Literature Review
Authors: Sirbu Carmen Adella , Ivan Raluca , Vasile Titus Mihai , Eftimie Lucian George , Costache Daniel Octavian
Published: June 2022

Background and aims. Multiple sclerosis is a disease of the central nervous system, whose treatment often involves the use of monoclonal antibodies. This can lead to a series of complications that the clinician should pay attention to and accordingly adjust the therapy. We aim to emphasize real-life experiences with adverse cutaneous reactions to monoclonal antibodies by presenting a series of two cases from our clinic. Methods. In the first case, a female patient was treated with natalizumab for eight years and developed relapsing-remitting cutaneous lesions following the monthly administration of the treatment. The second case is of a male patient treated with ocrelizumab, who developed plaque-like lesions following the fifth administration. We analyzed the biological parameters and performed investigations, dermatological evaluation and skin biopsies. Results. The result of the skin biopsy for the natalizumab patient showed a chronic spongiotic dermatitis, with the anti-natalizumab antibodies being negative. The patient who received ocrelizumab developed nummular eczema, disseminated on his trunk and limbs. Conclusions. Given the fact that these therapies are frequently used in multiple sclerosis patients, and their skin adverse reactions are known, we described some particularities and a brief review of the literature with practical implications. Further studies need to be conducted to establish a firm association between monoclonal antibodies therapy and adverse cutaneous reactions, but the clinician should be aware of their existence.
Femoral and Lateral Femoral Cutaneous Nerve Block as Anesthesia for High-Risk Intertrochanteric Fracture Repair Patients
Authors: Klimkiewicz Jakub , Klimkiewicz Anna , Gutowski Mateusz , Rustecki Bartosz , Kochanowski Dymitr , Ryczek Robert , Lubas Arkadiusz
Published: June 2022

Introduction. Intertrochanteric fracture (IF) is a common injury among the elderly. Due to significant comorbidities, anesthesia for IF repair may be challenging. The authors propose femoral nerve block together with a lateral femoral cutaneous nerve block and sedation as an anesthetic technique for most severe cases of IF with contraindications to spinal anesthesia. Methods. In total, 61 patients were enrolled prospectively in a study, 19 received general anesthesia (GA group), 22 spinal anesthesia (SA group), and 20 nerve blocks with sedation (PNB group). Results. Groups were comparable in terms of age, gender, independence, and several comorbidities: diabetes, obesity, underweight, cardiovascular, and cerebrovascular incidents in the past, dementia, chronic obstructive pulmonary disease, and frailty. Heart failure (p = 0.033), hemoglobin < 10 g/dL (p = 0.001) and eGFR < 30 mL/min (p = 0.039) were more frequent in PNB group. PNB group had higher American Society of Anesthesiologists (ASA) (p < 0.001), Nottingham Hip Fracture Score (NHFS) (p < 0.001), and Charlson Comorbidity Index (CCI) (p = 0.002) scales scores, and lower probability of 10-year survival according to CCI (p = 0.012). GA group had more frequent active malignancy (p = 0.041). GA and PNB groups had a higher frequency of hemostasis disorder (p < 0.001). Surgery was completed under the scheduled anesthesia technique. Survival, frequency of cardio and cerebrovascular incidents after surgery, loss of independence, and postoperative delirium were comparable between groups, as well as the length of postoperative stay. Conclusions. Surgical repair of intertrochanteric fracture with intramedullary nailing system among elderly, frail, and sick patients can be conducted under peripheral nerve block. FNB and LFCNB in the combination is a viable option for IT fracture repair.
DJ-1 Alleviates Neuroinflammation and the Related Blood-Spinal Cord Barrier Destruction by Suppressing NLRP3 Inflammasome Activation via SOCS1/Rac1/ROS Pathway in a Rat Model of Traumatic Spinal Cord Injury
Authors: Cai Lingxin , Gao Liansheng , Zhang Guoqiang , Zeng Hanhai , Wu Xinyan , Tan Xiaoxiao , Qian Cong , Chen Gao
Published: June 2022

DJ-1 has been shown to play essential roles in neuronal protection and anti-inflammation in nervous system diseases. This study aimed to explore how DJ-1 regulates neuroinflammation after traumatic spinal cord injury (t-SCI). The rat model of spinal cord injury was established by the clamping method. The Basso, Beattie, Bresnahan (BBB) score and the inclined plane test (IPT) were used to evaluate neurological function. Western blot was then applied to test the levels of DJ-1, NLRP3, SOCS1, and related proinflammatory factors (cleaved caspase 1, IL-1β and IL-18); ROS level was also examined. The distribution of DJ-1 was assessed by immunofluorescence staining (IF). BSCB integrity was assessed by the level of MMP-9 and tight junction proteins (Claudin-5, Occludin and ZO-1). We found that DJ-1 became significantly elevated after t-SCI and was mainly located in neurons. Knockdown of DJ-1 with specific siRNA aggravated NLRP3 inflammasome-related neuroinflammation and strengthened the disruption of BSCB integrity. However, the upregulation of DJ-1 by Sodium benzoate (SB) reversed these effects and improved neurological function. Furthermore, SOCS1-siRNA attenuated the neuroprotective effects of DJ-1 and increased the ROS, Rac1 and NLRP3. In conclusion, DJ-1 may alleviate neuroinflammation and the related BSCB destruction after t-SCI by suppressing NLRP3 inflammasome activation by SOCS1/Rac1/ROS pathways. DJ-1 shows potential as a feasible target for mediating neuroinflammation after t-SCI.
Prevalence of Obesity and Severe Obesity among Professionally Active Adult Population in Poland and Its Strong Relationship with Cardiovascular Co-Morbidities-POL-O-CARIA 2016–2020 Study
Authors: Rulkiewicz Anna , Pilchowska Iwona , Lisik Wojciech , Pruszczyk Piotr , Ciurzyński Michał , Domienik-Karłowicz Justyna
Published: June 2022

For several decades, a steady increase in the percentage of overweight and obese people has been observed all over the world. There are many studies available in the literature emphasizing the relationship of overweight and obesity with the occurrence of other diseases. The aim of this study is to characterize the prevalence of obesity and severe obesity, as well as their changes over time, among professionally active adults who underwent occupational medicine examinations in Poland in 2016–2020, for the POL-O-CARIA 2016–2020 study. In total, the results of 1,450,455 initial, control and periodic visits as part of the occupational medicine certificate were analyzed. Statistical calculations were performed with the use of IBM SPSS Statistics 25. In both groups (men/women), a significant decrease was observed every year for people who had normal body weight. In addition, the tendency to increase in people with I and III degrees of obesity was more strongly observed in the male group. A significant relationship was also observed between BMI categories and the occurrence of all analyzed comorbidities: hypertension, type 2 diabetes, lipid disorders and coronary artery disease (chi2 (70) = 12,228.11; p < 0.001). Detailed results showed that in the group of patients diagnosed with hypertension or lipid disorders, significant differences were observed between all groups; it turned out that as the BMI level increased (I, I, III), there was an increase in the percentage of occurrence of hypertension (38.1%, 41% and 45.3%, respectively) and type 2 diabetes (3.2%, 4.6% and 5.8%, respectively) (p < 0.001). Our analysis indicates that the prevalence of adult obesity and severe obesity will continue to increase nationwide, with an accompanying large increase in comorbidities.
Immunophenotypic Characteristics of Bone Marrow Microenvironment Cellular Composition at the Biochemical Progression of Multiple Myeloma
Authors: Krzywdzińska Agnieszka , Puła Bartosz , Szymczak Donata , Milanowska Aneta , Szeremet Agnieszka , Jamroziak Krzysztof
Published: June 2022

Multiple myeloma (MM) relapses are inevitable in the majority of patients, and in addition to genetic changes in the MM clone, the immune profile of the bone marrow (BM) plays a key role in this process. Biochemical progression or relapse (BR) precedes clinical relapse in a significant proportion of patients with MM. In the present study, we used flow cytometry to assess the cellular composition of the BM microenvironment in MM patients with confirmed BR. Fifteen distinct cells subsets in the BM were evaluated with the panel of antibodies used routinely for MRD monitoring in MM in 52 patients with MM (MRD-negative n = 20, BR n = 20, and clinically relapsed MM, RMM n = 12). The median percentage of MM cells detected in BR patients was 0.90% versus not detectable in MRD-negative patients and of 3.0% in RMM cohort. Compared to the MRD-negative group, BR status was associated with an increase in the percentage of lymphoid subpopulations, including memory B cells (p = 0.003), CD27+T cells (p = 0.002), and NK/NKT cells (p < 0.001). Moreover, a decrease in B-cell precursors (p < 0.001) and neutrophils (p = 0.006) was observed. There were no significant differences in the composition of the BM cell subpopulations between the BR and RMM groups. Our results indicate the involvement of B-, T-, and NK cells in the process of losing immune surveillance over the MM clone that leads to relapse. It can be speculated that similar studies of a larger cohort of BR patients can potentially identify a group of patients for which an early treatment intervention would be beneficial.
Delirium in ICU Patients after Cardiac Arrest: A Scoping Review
Authors: Mędrzycka-Dąbrowska Wioletta , Lange Sandra , Religa Dorota , Dąbrowski Sebastian , Friganović Adriano , Oomen Ber , Krupa Sabina
Published: June 2022

Introduction: The incidence of delirium in the intensive care unit is high, although it may differ according to the specific characteristics of the unit. Despite the rapid development of research on delirium in recent years, the pathophysiological mechanisms leading to the clinical presentation of delirium are still subject to hypotheses. The aim of this review was to describe the incidence of delirium in cardiac arrest survivors and the clinical impact of delirium on patient outcomes. Methods: A scoping review was conducted in the second quarter of 2022. The number of articles retrieved during each search test was limited to studies conducted between 2010 and 2020. Strict inclusion and exclusion criteria were applied. The last search was conducted in May 2022. Results: A total of 537 records was initially obtained from the databases. After discarding duplicates, selecting titles and abstracts, and then analyzing full-text articles, 7 studies met the inclusion criteria. The incidence of delirium in the cardiac arrest survivor population ranged from 8% to as high as 100%. The length of stay in ICU and hospital was significantly longer in patients with delirium than those without. Ninety-eight percent of patients had cognitive or perceptual impairment and psychomotor impairment. Of the seven studies included in the analysis, the RASS, CAM, and NuDesc scales were used to diagnose delirium. Potential risk factors that may influence the duration of delirium include age and time since resuscitation; propofol use shortened the duration of delirium. Conclusion: the incidence of delirium in ICU patients who survived CA is high. Cardiac arrest is an additional predisposing factor for delirium. In cardiac arrest survivors, the occurrence of delirium prolongs the duration of ICU and hospital stay and adversely affects functional outcomes. The most common type of delirium among this population was hypoactive delirium. A large percentage of patients manifested symptoms such as cognitive or perception impairment, psychomotor impairment, and impaired concentration and attention.
Impact of l-Carnitine Supplementation on Liver Enzyme Normalization in Patients with Chronic Liver Disease: A Meta-Analysis of Randomized Trials
Authors: Oh Hyunwoo , Park Chan Hyuk , Jun Dae Won
Published: June 2022

The effectiveness of l-carnitine in chronic liver disease remains controversial. We conducted this meta-analysis to assess the efficacy of various forms of l-carnitine in the treatment of chronic liver disease. Methods: We searched the Cochrane Library, EMBASE, KMBASE, and Medline databases for all relevant studies published until April 2022 that examined the ability of l-carnitine or its derivatives to normalize liver enzymes in patients with chronic liver disease. We performed meta-analyses of the proportion of patients with alanine aminotransferase (ALT) normalization and post-treatment serum aspartate aminotransferase (AST) and ALT levels. A random effects model was used for meta-analyses. Results: Fourteen randomized controlled trials (1217 patients) were included in this meta-analysis. The proportion of patients in whom ALT normalized was higher in the carnitine-orotate treatment group than in the control group (pooled odds ratio (OR), 95% confidence interval (CI) = 4.61 (1.48–14.39)). The proportion of patients in whom ALT normalized was also higher among those who received the carnitine-orotate complex, a combination of carnitine-orotate, biphenyl dimethyl dicarboxylate, and other minor supplementary compounds than in those who did not without significant heterogeneity (pooled OR (95% CI) = 18.88 (7.70–46.27); df = 1; p = 0.51; I2 = 0%). l-carnitine supplementation effectively lowered serum ALT levels compared to controls (pooled mean difference (95% CI) = −11.99 (−22.48 to −1.49)). Conclusions: l-carnitine supplementation significantly lowered ALT and AST levels and normalized ALT levels in patients with chronic liver disease.
Effects of Iloprost on Arterial Oxygenation and Lung Mechanics during One-Lung Ventilation in Supine-Positioned Patients: A Randomized Controlled Study
Authors: Lee Kyuho , Kim Mina , Kim Namo , Kang Su Jeong , Oh Young Jun
Published: June 2022

Patients undergoing one-lung ventilation (OLV) in the supine position face an increased risk of intraoperative hypoxia compared with those in the lateral decubitus position. We hypothesized that iloprost (ILO) inhalation improves arterial oxygenation and lung mechanics. Sixty-four patients were enrolled and allocated to either the ILO or control group (n = 32 each), to whom ILO or normal saline was administered. The partial pressure of the arterial oxygen/fraction of inspired oxygen (PaO2/FiO2) ratio, dynamic compliance, alveolar dead space, and hemodynamic variables were assessed 20 min after anesthesia induction with both lungs ventilated (T1) and 20 min after drug nebulization in OLV (T2). A linear mixed model adjusted for group and time was used to analyze repeated variables. While the alveolar dead space remained unchanged in the ILO group, it increased at T2 in the control group (n = 30 each) (p = 0.002). No significant differences were observed in the heart rate, mean blood pressure, PaO2/FiO2 ratio, or dynamic compliance in either group. Selective ILO nebulization was inadequate to enhance oxygenation parameters during OLV in the supine position. However, it favorably affected alveolar ventilation during OLV in supine-positioned patients without adverse hemodynamic effects.
1,1′-{[3,5-Bis(dodecyloxycarbonyl)-4-(naphthalen-2-yl)-1,4-dihydropyridine-2,6-diyl]bis(methylene)}bis{4-[(E)-2-(naphthalen-2-yl)vinyl]pyridin-1-ium}dibromide
Authors: Rucins Martins , Kaukulis Martins , Plotniece Aiva , Pajuste Karlis , Pikun Nadiia , Sobolev Arkadij
Published: June 2022

Synthesis of a double-charged cationic amphiphilic 1,4-dihydropyridine derivative with dodecyl ester groups at positions 3 and 5 of the 1,4-DHP ring was performed starting from Hantzsch type cyclization of dodecyl acetoacetate, 2-naphthaldehyde and ammonium acetate. Bromination of this compound followed by nucleophilic substitution of bromine with (E)-4-(2-(naphthalen-2-yl)vinyl)pyridine gave the desired cationic amphiphilic 1,1′-{[3,5-bis(dodecyloxycarbonyl)-4-(naphthalen-2-yl)-1,4-dihydropyridine-2,6-diyl]bis(methylene)}bis{4-[(E)-2-(naphthalen-2-yl)vinyl]pyridin-1-ium}dibromide. The obtained target compound was fully characterized by IR, UV, 1H-NMR, 13C-NMR, HRMS and microanalysis. The characterization of the cationic 1,4-DHP nanoparticles in an aqueous solution was performed by DLS measurements. The obtained results showed that the compound formed nanoparticles with an average diameter of around 300 nm, a PDI value of around 490 and a zeta-potential of around 20 mV for freshly prepared samples. However, after one week of storage at room temperature, an aggregation of nanoparticles was detected.
Effect of Silver Diamine Fluoride and Potassium Iodide Solution on Enamel Remineralization and Discoloration in Artificial Caries
Authors: Lee Ko Eun , Erdenebulgan Munkhulzii , Kang Chung-Min , Jung Hoi-In , Song Je Seon
Published: June 2022

Silver diamine fluoride (SDF) is a strong fluoride agent for caries control, remineralization, and reducing the incidence of dental caries. This study used 38% SDF with potassium iodide (KI) on enamel remineralization and compared it with the currently used 38% SDF and 5% sodium fluoride (NaF) varnish to treat artificial caries. Bovine incisors were demineralized for 120 h and randomly distributed for treatment by the remineralization agents for 120 h as follows: control (n = 15), SDF/KI (n = 15), SDF (n = 15), and NaF (n = 15). Colorimetric analysis was performed using the ΔE value. The Vickers microhardness (VHN) was determined to evaluate the enamel remineralization of the specimens. Polarized light microscopy (PLM) and scanning electron microscopy (SEM) were performed to confirm the surfaces precisely and histologically. SDF/KI caused no significant difference in discoloration between the control and NaF groups. There was also no significant difference in the VHN after remineralization, but SDF/KI exhibited the highest mean microhardness. PLM revealed that SDF/KI had the greatest remineralization ability. In conclusion, SDF/KI is effective for dental enamel remineralization even with KI, which reduces discoloration.
Mechanical Behavior of Shape Memory Alloy Fibers Embedded in Engineered Cementitious Composite Matrix under Cyclic Pullout Loads
Authors: Yang Zhao , Du Yalong , Liang Yujia , Ke Xiaolong
Published: June 2022

The incorporation of superelastic shape memory alloy (SMA) fibers into engineered cementitious composite (ECC) materials can provide high seismic energy dissipation and deformation self-centering capabilities for ECC materials. Whether the SMA fibers can be sufficiently bonded or anchored in the ECC matrix and whether the mechanical properties of the SMA fibers in the ECC matrix can be effectively utilized are the key scientific issues that urgently need to be studied. In order to study the mechanical behavior of SMA fiber embedded in ECC matrix, four groups of semi-dog-bone pullout specimens were fabricated, and the cyclic pullout tests were conducted in this paper. The pullout stress, displacement, and self-centering capability were analyzed, and different influencing factors were discussed. The results show that the knotted ends can provide sufficient anchorage force for SMA fibers, and the maximum pullout stress of SMA fiber can reach 1100 MPa, thus the superelasticity can be effectively stimulated. The SMA fibers show excellent self-centering capability in the test. The minimum residual deformation in the test is only 0.29 mm, and the maximum self-centering ratio can reach 0.93. Increasing bond length can increase the ultimate strain of SMA fibers with knotted ends, but reduce the maximum pullout stress. Increasing fiber diameter can increase both the ultimate strain and the maximum stress of knotted end SMA fibers. While neither bond length nor fiber diameter has significant effect on the self-centering ratio. This paper provides a theoretical basis for further study of the combination of SMA fibers and ECC materials.
A Feasibility Study of High-Entropy Alloy Coating Deposition by Detonation Spraying Combined with Laser Melting
Authors: Batraev Igor S. , Ulianitsky Vladimir Yu. , Sova Alexey A. , Samodurova Marina N. , Trofimov Evgeny A. , Pashkeev Kirill Yu. , Malikov Alexander G. , Dudina Dina V. , Ukhina Arina V.
Published: June 2022

In this work, a new two-stage approach to the deposition of high-entropy alloy coatings is proposed. At the first stage, a composite precursor coating is formed by detonation spraying of the metal powder mixtures. At the second stage, the precursor coating is re-melted by a laser, and the formation of multi-component solid solution phases can be expected upon solidification. The feasibility of the proposed approach was validated using three different mixtures of Fe, Ni, Cu, Co and Al powders. It was shown that detonation spraying allows forming composite coatings with a uniform distribution of the lamellae of different metals. The results of the structural analysis of the laser-treated coatings suggest that complete alloying occurred in the melt and face-centered cubic solid solutions formed in the coatings upon cooling.
Mechanical Deformation Analysis of a Flexible Finger in Terms of an Improved ANCF Plate Element
Authors: Xing Yu , Liu Lei , Liu Chao , Li Bo , Wang Zishen , Li Pengfei , Zhang Erhu
Published: June 2022

In recent years, flexible continuum robots have been substantially developed. Absolute nodal coordinates formulation (ANCF) gives a feasible path for simulating the behavior of flexible robots. However, the model of finger-shaped robots is often regarded as a cylinder and characterized by a beam element. Obviously, this is short of characterizing the geometrical feature of fingers in detail, especially under bending conditions. Additionally, for the lower-order plate element, it is hard to characterize the bending behavior of the flexible finger due to fewer nodes; a higher-order plate element often requires an extremely long computing time. In this work, an improved ANCF lower-order plate element is used to increase the accuracy of the Yeoh model and characterize the geometrical structure of silicone rubber fingers, taking into particular consideration the effect of volume locks and multi-body system constraints. Since it is a kind of lower-order plate element, essentially, the computing time is nearly the same as that of conventional lower-order plate elements. The validity of this model was verified by comparing it with the results of the published reference. The flexible finger, manufactured using silicone rubber, is characterized by the novel ANCF lower-order plate element, whereby its mechanical deformation and bending behavior are simulated both efficiently and accurately. Compared to the ANCF beam element, conventional lower-order plate element, and higher-order plate element, the novel plate element in this paper characterizes the external contour of the finger better, reflects bending behavior more realistically, and converges in less computing time.
Fault Detection of Bearing by Resnet Classifier with Model-Based Data Augmentation
Authors: Qian Lu , Pan Qing , Lv Yaqiong , Zhao Xingwei
Published: June 2022

It is always an important and challenging issue to achieve an effective fault diagnosis in rotating machinery in industries. In recent years, deep learning proved to be a high-accuracy and reliable method for data-based fault detection. However, the training of deep learning algorithms requires a large number of real data, which is generally expensive and time-consuming. To cope with this, we proposed a Resnet classifier with model-based data augmentation, which is applied for bearing fault detection. To this end, a dynamic model was first established to describe the bearing system by adjusting model parameters, such as speed, load, fault size, and the different fault types. Large amounts of data under various operation conditions can then be generated. The training dataset was constructed by the simulated data, which was then applied to train the Resnet classifier. In addition, in order to reduce the gap between the simulation data and the real data, the envelop signals were used instead of the original signals in the training process. Finally, the effectiveness of the proposed method was demonstrated by the real bearing experimental data. It is remarkable that the application of the proposed method can be further extended to other mechatronic systems with a deterministic dynamic model.
Solving Inverse Conductivity Problems in Doubly Connected Domains by the Homogenization Functions of Two Parameters
Authors: Lu Jun , Shi Lianpeng , Liu Chein-Shan , Chen C. S.
Published: June 2022

In the paper, we make the first attempt to derive a family of two-parameter homogenization functions in the doubly connected domain, which is then applied as the bases of trial solutions for the inverse conductivity problems. The expansion coefficients are obtained by imposing an extra boundary condition on the inner boundary, which results in a linear system for the interpolation of the solution in a weighted Sobolev space. Then, we retrieve the spatial- or temperature-dependent conductivity function by solving a linear system, which is obtained from the collocation method applied to the nonlinear elliptic equation after inserting the solution. Although the required data are quite economical, very accurate solutions of the space-dependent and temperature-dependent conductivity functions, the Robin coefficient function and also the source function are available. It is significant that the nonlinear inverse problems can be solved directly without iterations and solving nonlinear equations. The proposed method can achieve accurate results with high efficiency even for large noise being imposed on the input data.
Removal of Ni(II) Ions by Poly(Vinyl Alcohol)/Al2O3 Nanocomposite Film via Laser Ablation in Liquid
Authors: Alkallas Fatemah H. , Ahmed Hoda A. , Alrebdi Tahani A. , Pashameah Rami Adel , Alrefaee Salhah H. , Alsubhe Emaan , Trabelsi Amira Ben Gouider , Mostafa Ayman M. , Mwafy Eman A.
Published: June 2022

Al2O3-poly(vinyl alcohol) nanocomposite (Al2O3-PVA nanocomposite) was generated in a single step using an eco-friendly method based on the pulsed laser ablation approach immersed in PVA solution to be applicable for the removal of Ni(II) from aqueous solution, followed by making a physicochemical characterization by SEM, XRD, FT-IR, and EDX. After that, the effect of adsorption parameters, such as pH, contact time, initial concentration of Ni(II), and medium temperature, were investigated for removal Ni(II) ions. The results showed that the adsorption was increased when pH was 5.3, and the process was initially relatively quick, with maximum adsorption detected within 90 min of contact time with the endothermic sorption process. Moreover, the pseudo-second-order rate kinetics (k2 = 9.9 × 10−4 g mg−1 min−1) exhibited greater agreement than that of the pseudo-first-order. For that, the Ni(II) was effectively collected by Al2O3-PVA nanocomposite prepared by an eco-friendly and simple method for the production of clean water to protect public health.
Correction: You, K.H.; Kim, H.-K. A Study on the Effect of Process and Material Variables on the Hot Stamping Formability of Automotive Body Parts. Metals 2021, 11, 1029
Authors: You Kang Ho , Kim Heung-Kyu
Published: June 2022

Data and expressions provided by Hyundai Steel, which are not intended to be disclosed, were included in the figures and text [...]
Effect of Ultrasonic Vibration in Friction Stir Welding of 2219 Aluminum Alloy: An Effective Model for Predicting Weld Strength
Authors: Xue Fei , He Diqiu , Zhou Haibo
Published: June 2022

Friction stir welding (FSW) is today used as a premier solution for joining non-ferrous metals, although there are many limitations in its application. One of the objectives of this study was to propose an innovative welding technique, namely ultrasonic-assisted friction stir welding (UAFSW) with longitudinal ultrasonic vibration applied to the stirring head. In this paper, UAFSW mechanical properties and microstructure analysis were performed to demonstrate that the fluidity of the weld area was improved and the strengthened phase organization was partially preserved, due to the application of ultrasonic vibration. The addition of 1.8 kW of ultrasonic vibration at 1200 rpm and 150 mm/min welding parameters resulted in a 10.5% increase in the tensile strength of the weld. The ultimate tensile strength of 2219 aluminum alloy UAFSW was analyzed and predicted using mathematical modeling and machine learning techniques. A full factorial design method with multiple regression, random forest, and support vector machine was used to validate the experimental results. In predicting the tensile behavior of UAFSW joints, by comparing the evaluation metrics, such as R2, MSE, RMSE, and MAE, it was found that the RF model was 22% and 21% more accurate in the R2 metric compared to other models, and RF was considered as the best performing machine learning method.
PPARβ/δ Augments IL-1β-Induced COX-2 Expression and PGE2 Biosynthesis in Human Mesangial Cells via the Activation of SIRT1
Authors: Li Yaqing , Cao Rong , Gu Tingting , Cao Cong , Chen Tingyue , Guan Youfei , Zhang Xiaoyan
Published: June 2022

Peroxisome proliferator-activated receptor β/δ (PPARβ/δ), a ligand-activated nuclear receptor, regulates lipid and glucose metabolism and inflammation. PPARβ/δ can exert an anti-inflammatory effect by suppressing proinflammatory cytokine production. Cyclooxygenase-2 (COX-2)-triggered inflammation plays a crucial role in the development of many inflammatory diseases, including glomerulonephritis. However, the effect of PPARβ/δ on the expression of COX-2 in the kidney has not been fully elucidated. The present study showed that PPARβ/δ was functionally expressed in human mesangial cells (hMCs), where its expression was increased by interleukin-1β (IL-1β) treatment concomitant with enhanced COX-2 expression and prostaglandin E2 (PGE2) biosynthesis. The treatment of hMCs with GW0742, a selective agonist of PPARβ/δ, or the overexpression of PPARβ/δ via an adenovirus-mediated approach significantly increased COX-2 expression and PGE2 production. PPARβ/δ could further augment the IL-1β-induced COX-2 expression and PGE2 production in hMCs. Moreover, both PPARβ/δ activation and overexpression markedly increased sirtuin 1 (SIRT1) expression. The inhibition or knockdown of SIRT1 significantly attenuated the effects of PPARβ/δ on the IL-1β-induced expression of COX-2 and PGE2 biosynthesis. Taken together, PPARβ/δ could augment the IL-1β-induced COX-2 expression and PGE2 production in hMCs via the SIRT1 pathway. Given the critical role of COX-2 in glomerulonephritis, PPARβ/δ may represent a novel target for the treatment of renal inflammatory diseases.
Untargeted Metabolomics Reveal Parenteral Nutrition-Associated Alterations in Pediatric Patients with Short Bowel Syndrome
Authors: Wang Ying , Liu Yang , Gao Bei , Yan Junkai , Cai Wei , Jiang Lu
Published: June 2022

Short bowel syndrome (SBS) is a major cause of intestinal failure (IF) that may require long-term parenteral nutrition (PN) support. However, long-term PN is accompanied by severe complications such as catheter-related blood stream infection (CRBSI) and intestinal failure-associated liver disease (IFALD), and it is associated with high healthcare costs. In this study, we characterized the plasma metabolomic profile and investigated the role of metabolism in predicting long-term PN in pediatric patients with SBS. Untargeted metabolomics was performed in plasma samples from 20 SBS patients with PN support: 6 patients had IFALD and 14 patients had no liver disease. As controls, 18 subjects without liver or intestinal diseases were included for the analysis. SBS patients had distinct plasma metabolomic signatures compared to controls, and several pathways associated with amino acid metabolism and cell death were significantly changed. The presence of IFALD in SBS was associated with alterations of metabolites mainly classified as “amino acids, peptides, and analogues” and “benzene and derivatives”. Serum direct bilirubin levels were negatively correlated with levels of uridine, skatole, and glabrol. Importantly, SBS patients with long-term PN showed significantly increased levels of glutamine compared to those in the short-term PN group. Finally, using multivariate logistic regression analysis, we developed a prediction model including glutamine and creatinine to identify pediatric SBS patients who need long-term PN support. These findings underscore the potential key role of the metabolome in SBS with IF and suggest that metabolomic profiles could be used in long-term PN assessment.
Retardation of Bacterial Biofilm Formation by Coating Urinary Catheters with Metal Nanoparticle-Stabilized Polymers
Authors: Rugaie Osamah Al , Abdellatif Ahmed A. H. , El-Mokhtar Mohamed A. , Sabet Marwa A. , Abdelfattah Ahmed , Alsharidah Mansour , Aldubaib Musaed , Barakat Hassan , Abudoleh Suha Mujahed , Al-Regaiey Khalid A. , Tawfeek Hesham M.
Published: June 2022

Urinary catheter infections remain an issue for many patients and can complicate their health status, especially for individuals who require long-term catheterization. Catheters can be colonized by biofilm-forming bacteria resistant to the administered antibiotics. Therefore, this study aimed to investigate the efficacy of silver nanoparticles (AgNPs) stabilized with different polymeric materials generated via a one-step simple coating technique for their ability to inhibit biofilm formation on urinary catheters. AgNPs were prepared and characterized to confirm their formation and determine their size, charge, morphology, and physical stability. Screening of the antimicrobial activity of nanoparticle formulations and determining minimal inhibitory concentration (MIC) and their cytotoxicity against PC3 cells were performed. Moreover, the antibiofilm activity and efficacy of the AgNPs coated on the urinary catheters under static and flowing conditions were examined against a clinical isolate of Escherichia coli. The results showed that the investigated polymers could form physically stable AgNPs, especially those prepared using polyvinyl pyrrolidone (PVP) and ethyl cellulose (EC). Preliminary screening and MIC determinations suggested that the AgNPs-EC and AgNPs-PVP had superior antibacterial effects against E. coli. AgNPs-EC and AgNPs-PVP inhibited biofilm formation to 58.2% and 50.8% compared with AgNPs-PEG, silver nitrate solution and control samples. In addition, coating urinary catheters with AgNPs-EC and AgNPs-PVP at concentrations lower than the determined IC50 values significantly (p < 0.05; t-test) inhibited bacterial biofilm formation compared with noncoated catheters under both static and static and flowing conditions using two different types of commercial Foley urinary catheters. The data obtained in this study provide evidence that AgNP-coated EC and PVP could be useful as potential antibacterial and antibiofilm catheter coating agents to prevent the development of urinary tract infections caused by E. coli.
Acne, Microbiome, and Probiotics: The Gut–Skin Axis
Authors: Sánchez-Pellicer Pedro , Navarro-Moratalla Laura , Núñez-Delegido Eva , Ruzafa-Costas Beatriz , Agüera-Santos Juan , Navarro-López Vicente
Published: June 2022

The objective of this narrative review was to check the influence of the human microbiota in the pathogenesis of acne and how the treatment with probiotics as adjuvant or alternative therapy affects the evolution of acne vulgaris. Acne is a chronic inflammatory skin disease involving the pilosebaceous units. The pathogenesis of acne is complex and multifactorial involving genetic, metabolic, and hormonal factors in which both skin and gut microbiota are implicated. Numerous studies have shown the bidirectionality between the intestinal microbiota and skin homeostasis, a communication mainly established by modifying the immune system. Increased data on the mechanisms of action regarding the relevance of Cutibacterium acnes, as well as the importance of the gut–skin axis, are becoming known. Diverse and varied in vitro studies have shown the potential beneficial effects of probiotics in this context. Clinical trials with both topical and oral probiotics are scarce, although they have shown positive results, especially with oral probiotics through the modulation of the intestinal microbiota, generating an anti-inflammatory response and restoring intestinal integrity, or through metabolic pathways involving insulin-like growth factor I (IGF-1). Given the aggressiveness of some standard acne treatments, probiotics should continue to be investigated as an alternative or adjuvant therapy.
Characterization of Roasting Time on Sensory Quality, Color, Taste, and Nonvolatile Compounds of Yuan An Yellow Tea
Authors: Ye Fei , Qiao Xiaoyan , Gui Anhui , Liu Panpan , Wang Shengpeng , Wang Xueping , Teng Jin , Zheng Lin , Feng Lin , Han Hanshan , Zhang Binghua , Chen Xun , Gao Zhiming , Gao Shiwei , Zheng Pengcheng
Published: June 2022

Roasting is crucial for producing Yuan An yellow tea (YAYT) as it substantially affects sensory quality. However, the effect of roasting time on YAYT flavor quality is not clear. To investigate the effect of roasting time on the sensory qualities, chemical components, odor profiles, and metabolic profile of YAYTs produced with 13 min roasting, 16 min roasting, 19 min roasting, 22 min roasting, and 25 min roasting were determined. The YAYTs roasted for 22 min got higher sensory scores and better chemical qualities, such as the content of gallocatechin (GC), gallocatechin gallate (GCG), free amino acids, solutable sugar, meanwhile the lightness decreased, the hue of tea brew color (b) increased, which meant the tea brew got darker and yellower. YAYTs roasted for 22 min also increased the contents of key odorants, such as benzaldehyde, nonanal, β-cyclocitral, linalool, nerol, α-cedrol, β-ionone, limonene, 2-methylfuran, indole, and longiborneol. Moreover, non-targeted metabolomics identified up to 14 differentially expressed metabolites through pair-wise comparisons, such as flavonoids, phenolic acids, sucrose, and critical metabolites, which were the main components corresponding to YAYT roasted for 22 min. In summary, the current results provide scientific guidance for the production of high quality YAYT.
Changes in Isoflavone Profile from Soybean Seeds during Cheonggukjang Fermentation Based on High-Resolution UPLC-DAD-QToF/MS: New Succinylated and Phosphorylated Conjugates
Authors: Lee Suji , Kwon Ryeong Ha , Kim Ju Hyung , Na Hyemin , Lee So-Jeong , Choi Yu-Mi , Yoon Hyemyeong , Kim So Young , Kim Yong-Suk , Lee Sang Hoon , Yoo Seon Mi , Kim Heon-Woong , Wee Chi-Do
Published: June 2022

In this study, thirty-eight isoflavone derivatives were comprehensively identified and quantified from the raw, steamed and fermented seeds of four selected soybean cultivars based on UPLC-DAD-QToF/MS results with reference to the previously reported LC-MS library and flavonoid database, and summarized by acylated group including glucosides (Glu), malonyl-glucosides (Mal-Glu), acetyl-glucosides (Ac-Glu), succinyl-glucosides (Suc-Glu) and phosphorylated conjugates (Phos) in addition to aglycones. Among them, Suc-Glu and Phos derivatives were newly generated due to fermentation by B. subtilis AFY-2 (cheonggukjang). In particular, Phos were characterized for the first time in fermented soy products using Bacillus species. From a proposed roadmap on isoflavone-based biotransformation, predominant Mal-Glu (77.5–84.2%, raw) decreased rapidly by decarboxylation and deesterification into Ac-Glu and Glu (3.5–8.1% and 50.0–72.2%) during steaming, respectively. As fermentation continued, the increased Glu were mainly succinylated and phosphorylated as well as gradually hydrolyzed into their corresponding aglycones. Thus, Suc-Glu and Phos (17.3–22.4% and 1.5–5.4%, 36 h) determined depending on cultivar type and incubation time, and can be considered as important biomarkers generated during cheonggukjang fermentation. Additionally, the changes of isoflavone profile can be used as a fundamental report in applied microbial science as well as bioavailability research from fermented soy foods.
Cavitand Decorated Silica as a Selective Preconcentrator for BTEX Sensing in Air
Authors: Rozzi Andrea , Pedrini Alessandro , Pinalli Roberta , Cozzani Enrico , Elmi Ivan , Zampolli Stefano , Dalcanale Enrico
Published: June 2022

The monitoring of benzene and other carcinogenic aromatic volatile compounds at the ppb level requires boosting both the selectivity and sensitivity of the corresponding sensors. A workable solution is the introduction in the devices of preconcentrator units containing molecular receptors. In particular, quinoxaline cavitands (QxCav) resulted in very efficient preconcentrator materials for the BTEX in air to the point that they have been successfully implemented in a commercial sensor. In this work, we report a highly efficient quinoxaline-based preconcentrator material, in which the intrinsic adsorption capacity of the QxCav has been maximized. The new material consists of silica particles covalently coated with a suitable functionalized QxCav derivative (QxCav@SiO2). In this way, all the cavities are exposed to the analyte flux, boosting the performance of the resulting preconcentration cartridge well above that of the pure QxCav. It is noteworthy that the preconcentrator adsorption capacity is independent of the relative humidity of the incoming air.
Entropy Generation in 2D Lid-Driven Porous Container with the Presence of Obstacles of Different Shapes and under the Influences of Buoyancy and Lorentz Forces
Authors: Maneengam Apichit , Laidoudi Houssem , Abderrahmane Aissa , Rasool Ghulam , Guedri Kamel , Weera Wajaree , Younis Obai , Bouallegue Belgacem
Published: June 2022

This paper includes a numerical investigation of a hybrid fluid containing 4% of Al2O3-Cu nanoparticles in a lid-driven container. The upper wall of the container has a high temperature and is movable. The lower wall is cool and wavy. An obstacle is set in the middle of the container for its effect on thermal activity. The medium is permeable to the fluid, and the entire system is immersed in a fixed-effect magnetic field. The digital simulation is achieved using the technique of Galerkin finite element (GFEM) which solves the differential equations. This investigation aims to know the pattern of heat transfer between the lateral walls and the lower wall of the container through the intervention of a set of conditions and criteria, namely: the strength of the magnetic field changes in the range of (Ha = 0 to 100); the chamber porosity varies in the range of (Da = 10−5 to 10−2); the strength of buoyancy force is varied according to the Grashof number (Gr = 102 to 104); the cross-section of the baffle includes the following shapes—elliptical, square, triangular and circular; the surface of the lower wall contains waves; and the number changes (N = 2 to 8). Through this research, it was concluded that the triangular shape of the baffle is the best in terms of thermal activity. Also, increasing the number of lower-wall waves reduces thermal activity. For example, the change in the shape of the obstacle from the elliptical to triangular raises the value of Nu number at a rate of 15.54% for Ha = 0, N = 8, and Gr = 104.
Skipping Breakfast and Incidence of Frequent Alcohol Drinking in University Students in Japan: A Retrospective Cohort Study
Authors: Matsumura Yuichiro , Yamamoto Ryohei , Shinzawa Maki , Matsushita Taisuke , Yoshimura Ryuichi , Otsuki Naoko , Mizui Masayuki , Matsui Isao , Kaimori Junya , Sakaguchi Yusuke , Ishibashi Chisaki , Ide Seiko , Nakanishi Kaori , Nishida Makoto , Kudo Takashi , Yamauchi-Takihara Keiko , Nagatomo Izumi , Moriyama Toshiki
Published: June 2022

Frequency of alcohol drinking is a potential predictor of binge drinking of alcohol, a serious social problem for university students. Although previous studies have identified skipping breakfast as a predictor of various health-compromising behaviors and cardiometabolic diseases, few studies have assessed the association between skipping breakfast and the incidence of frequent alcohol drinking. This retrospective cohort study included 17,380 male and 8799 female university students aged 18–22 years admitted to Osaka universities between 2004 and 2015. The association between breakfast frequency (eating every day, skipping occasionally, and skipping often/usually) and the incidence of frequent alcohol drinking, defined as drinking ≥4 days/week, was assessed using multivariable-adjusted Cox proportional hazards models. During the median observational period of 3.0 years, 878 (5.1%) men and 190 (2.2%) women engaged in frequent alcohol drinking. Skipping breakfast was significantly associated with the incidence of frequent alcohol drinking (adjusted hazard ratios [95% confidence interval] of eating every day, skipping occasionally, and skipping often/usually: 1.00 [reference], 1.02 [0.84–1.25], and 1.48 [1.17–1.88] in men; 1.00 [reference], 1.60 [1.03–2.49], and 3.14 [1.88–5.24] in women, respectively). University students who skipped breakfast were at a higher risk of frequent alcohol drinking than those who ate breakfast every day.