bluuebunny/crossref_metadata_2025 huggingface dataset:
Amelioration Effects against Salinity Stress in Strawberry by Bentonite–Zeolite Mixture, Glycine Betaine, and Bacillus amyloliquefaciens in Terms of Plant Growth, Nutrient Content, Soil Properties, Yield, and Fruit Quality Characteristics
Authors: Ntanos Efstathios , Kekelis Panagiotis , Assimakopoulou Anna , Gasparatos Dionisios , Denaxa Nikoleta-Kleio , Tsafouros Athanassios , Roussos Petros Anargyrou
Published: September 2021

Strawberry, the most significant berry crop, is characterized as a salt-sensitive plant. The present study aimed to examine ways to alleviate salinity symptoms (34 mM of NaCl in irrigation water) in strawberry plants. For this purpose, the osmolyte glycine betaine was foliarly applied, a mixture of bentonite–zeolite was added to the substrate, and a microbial product based on Bacillys amyloliquefaciens as a soil drench were tested in terms of plant growth and nutrient status, yield, fruit physiological and organoleptic characteristics, as well as phytochemical properties (phenolic compounds, carbohydrates, organic acids, anthocyanins, and antioxidant capacity), and soil physicochemical properties. Salinity severely reduced plant growth and yield, while the effects on fruit quality were also distinct. Treatments alleviated to some extent these negative effects. Plant nutrient content was not severely affected by product application, and neither were most of the soil physicochemical properties. Among the products applied, the mixture of bentonite plus zeolite and glycine betaine proved to be more efficient in ameliorating toxicity symptoms, as both treatments preserved plant hydric status and plant growth, while glycine betaine resulted in an almost 30% higher yield than the treatment with saline water.
Real-Time Semantic Image Segmentation with Deep Learning for Autonomous Driving: A Survey
Authors: Papadeas Ilias , Tsochatzidis Lazaros , Amanatiadis Angelos , Pratikakis Ioannis
Published: September 2021

Semantic image segmentation for autonomous driving is a challenging task due to its requirement for both effectiveness and efficiency. Recent developments in deep learning have demonstrated important performance boosting in terms of accuracy. In this paper, we present a comprehensive overview of the state-of-the-art semantic image segmentation methods using deep-learning techniques aiming to operate in real time so that can efficiently support an autonomous driving scenario. To this end, the presented overview puts a particular emphasis on the presentation of all those approaches which permit inference time reduction, while an analysis of the existing methods is addressed by taking into account their end-to-end functionality, as well as a comparative study that relies upon a consistent evaluation framework. Finally, a fruitful discussion is presented that provides key insights for the current trend and future research directions in real-time semantic image segmentation with deep learning for autonomous driving.
Effects of Dietary Fishmeal Replacement by Poultry By-Product Meal and Hydrolyzed Feather Meal on Liver and Intestinal Histomorphology and on Intestinal Microbiota of Gilthead Seabream (Sparus aurata)
Authors: Psofakis Pier , Meziti Alexandra , Berillis Panagiotis , Mente Eleni , Kormas Konstantinos A. , Karapanagiotidis Ioannis T.
Published: September 2021

The effects on liver and intestinal histomorphology and on intestinal microbiota in gilthead seabream (Sparus aurata) fed diets that contained poultry by-product meal (PBM) and hydrolyzed feather meal (HFM) as fishmeal replacements were studied. Fish fed on a series of isonitrogenous and isoenergetic diets, where fishmeal protein of the control diet (FM diet) was replaced by either PBM or by HFM at 25%, 50% and 100% without amino acid supplementation (PBM25, PBM50, PBM100, HFM25, HFM50 and HFM100 diets) or supplemented with lysine and methionine (PBM25+, PBM50+, HFM25+ and HFM50+ diets). The use of PBM and HFM at 25% fishmeal replacement generated a similar hepatic histomorphology to FM-fed fish, indicating that both land animal proteins are highly digestible at low FM replacement levels. However, 50% and 100% FM replacement levels by either PBM or HFM resulted in pronounced hepatic alterations in fish with the latter causing more severe degradation of the liver. Dietary amino acid supplementation delivered an improved tissue histology signifying their importance at high FM replacement levels. Intestinal microbiota was dominated by Proteobacteria (58.8%) and Actinobacteria (32.4%) in all dietary groups, but no specific pattern was observed among them at any taxonomic level. This finding was probably driven by the high inter-individual variability observed.
Which Utterance Types Are Most Suitable to Detect Hypernasality Automatically?
Authors: Moreno-Torres Ignacio , Lozano Andrés , Nava Enrique , Bermúdez-de-Alvear Rosa
Published: September 2021

Automatic tools to detect hypernasality have been traditionally designed to analyze sustained vowels exclusively. This is in sharp contrast with clinical recommendations, which consider it necessary to use a variety of utterance types (e.g., repeated syllables, sustained sounds, sentences, etc.) This study explores the feasibility of detecting hypernasality automatically based on speech samples other than sustained vowels. The participants were 39 patients and 39 healthy controls. Six types of utterances were used: counting 1-to-10 and repetition of syllable sequences, sustained consonants, sustained vowel, words and sentences. The recordings were obtained, with the help of a mobile app, from Spain, Chile and Ecuador. Multiple acoustic features were computed from each utterance (e.g., MFCC, formant frequency) After a selection process, the best 20 features served to train different classification algorithms. Accuracy was the highest with syllable sequences and also with some words and sentences. Accuracy increased slightly by training the classifiers with between two and three utterances. However, the best results were obtained by combining the results of multiple classifiers. We conclude that protocols for automatic evaluation of hypernasality should include a variety of utterance types. It seems feasible to detect hypernasality automatically with mobile devices.
Expression of the Melatonin-Associated Genes in Fibroblasts That Have Been Co-Exposed to Fluoride and a Moderate-Strength Static Magnetic Field
Authors: Kruszniewska-Rajs Celina , Synowiec-Wojtarowicz Agnieszka , Gola Joanna , Kimsa-Dudek Magdalena
Published: September 2021

Fluoride can weaken the protective role of melatonin in reducing cellular damage. A static magnetic field is a physical factor that can counteract the negative effect of fluoride. Hence, the main objective of the study was to analyze the transcriptional activity of the genes that are associated with the activity of melatonin in human skin fibroblasts that have been co-exposed to fluoride and a moderate-strength static magnetic field. The expression of the melatonin-associated genes in human fibroblasts that had simultaneously been exposed to F− and a static magnetic field was determined using an oligonucleotide microarray and RT-qPCR techniques. The concentration of oxidative damage markers was also measured. In NaF and static magnetic field-treated cells, there was a tendency to compensate for the expression of the differentiating genes (IL27RA, NR1D1, RRP7A, YIPF1, HIST1H2BD) that had been modified by the presence of fluoride. It has been also shown that the oxidative damage marker concentration was statistically lower in the cells that had simultaneously been exposed to fluoride and a static magnetic field compared to the F-treated cells. In conclusion, the protective role of a moderate-strength static magnetic field on human dermal fibroblasts that had been exposed to fluoride was demonstrated, and its mechanism of action is associated with the melatonin-dependent pathways.
Keyword Extraction Algorithm for Classifying Smoking Status from Unstructured Bilingual Electronic Health Records Based on Natural Language Processing
Authors: Bae Ye Seul , Kim Kyung Hwan , Kim Han Kyul , Choi Sae Won , Ko Taehoon , Seo Hee Hwa , Lee Hae-Young , Jeon Hyojin
Published: September 2021

Smoking is an important variable for clinical research, but there are few studies regarding automatic obtainment of smoking classification from unstructured bilingual electronic health records (EHR). We aim to develop an algorithm to classify smoking status based on unstructured EHRs using natural language processing (NLP). With acronym replacement and Python package Soynlp, we normalize 4711 bilingual clinical notes. Each EHR notes was classified into 4 categories: current smokers, past smokers, never smokers, and unknown. Subsequently, SPPMI (Shifted Positive Point Mutual Information) is used to vectorize words in the notes. By calculating cosine similarity between these word vectors, keywords denoting the same smoking status are identified. Compared to other keyword extraction methods (word co-occurrence-, PMI-, and NPMI-based methods), our proposed approach improves keyword extraction precision by as much as 20.0%. These extracted keywords are used in classifying 4 smoking statuses from our bilingual EHRs. Given an identical SVM classifier, the F1 score is improved by as much as 1.8% compared to those of the unigram and bigram Bag of Words. Our study shows the potential of SPPMI in classifying smoking status from bilingual, unstructured EHRs. Our current findings show how smoking information can be easily acquired for clinical practice and research.
The Impact of Physical Education Classes on Health and Quality of Life during the COVID-19
Authors: Fang Jiceh-Der Dong , Teng Pi-Cheng , Wang Fong-Jia
Published: September 2021

The purpose of this study was to examine the effects of the COVID-19 pandemic on the well-being and the quality of life of college students participating in physical education classes. We consider this study to be relevant because during the COVID-19 pandemic we tested whether the boost in effective activity among physical education class participants affected well-being and quality of life. A sectional questionnaire survey was conducted across Taiwan in 2019–2020. Data were collected in two stages within 6 months from a sample of 1011 university students in Taiwan (328 male, 683 female). A series of one-way ANOVAs was adopted to examine each outcome across groups and time phases. The results provide support for a positive relationship between well-being (β = 0.25, p < 0.001), and quality of life (β = 0.92, p < 0.001), supporting our expectations. Findings from our study suggest that physical activity was positively associated with mental health; and participation in physical activity had an effect on the well-being and quality of life in college students.
Impacts of Projected Urban Expansion on Rainfall and Temperature during Rainy Season in the Middle-Eastern Region in Tanzania
Authors: Anande Doreen M. , Park Moon-Soo
Published: September 2021

Future changes of land use and land cover (LULC) due to urbanization can cause variations in the frequency and severity of extreme weather events, affecting local climate and potentially worsening impact of such events. This work examines the local climatic impacts associated with projected urban expansion through simulations of rainfall and temperature over the rapidly growing city of the middle-eastern region in Tanzania. Simulations were conducted using a mesoscale Weather Research and Forecasting (WRF) model for a period of 10 days during the rainfall season in April 2018. The Global Forecasting System data of 0.25° resolution was used to simulate the WRF model in two-way nested domains at resolutions of 12 km and 4 km correspondingly. Urban and built-up areas under the current state, low urbanization (30%), and high urbanization (99%) scenarios were taken into account as LULC categories. As the urbanized area increased, daily mean, maximum and minimum air temperatures, as well as precipitation increased. Local circulation affected the spatial irregularities of air temperature and precipitation. Results imply that urbanization can amplify the impacts of future climate changes dramatically. These results can be applicable to the city planning to minimize the adverse effect of urbanization on temperature and precipitation.
Quantum Confinement Effects of Thin Co3O4 Films
Authors: Barnasas Alexandros , Garoufalis Christos S. , Anyfantis Dimitrios I. , Bouropoulos Nikolaos , Poulopoulos Panagiotis , Hayrapetyan David B. , Baskoutas Sotirios
Published: September 2021

Thin Co films were deposited on quartz and Corning glass by radio frequency magnetron sputtering. The films were postannealed at 500 °C in a furnace in air atmosphere. The resulting samples were examined with X-ray diffraction experiments, which revealed that they consist of single-phase, polycrystalline Co3O4. The morphology of selected samples was recorded by atomic force microscopy. Ultraviolet-visible light absorption spectroscopy experiments probed the absorbance of the films in the wavelength range 200–1100 nm. Two types of transitions (energy gaps) were clearly identified. Both of them were found to be “blue shifted” with decreasing film thickness; this is interpreted as evidence of quantum confinement effects. For the case of the first gap value, this was corroborated by calculations based on a combination of the Potential Morphing Method and the effective mass approximation.
Molecular Determinants of Filament Capping Proteins Required for the Formation of Functional Flagella in Gram-Negative Bacteria
Authors: Nedeljković Marko , Postel Sandra , Pierce Brian G. , Sundberg Eric J.
Published: September 2021

Bacterial flagella are cell surface protein appendages that are critical for motility and pathogenesis. Flagellar filaments are tubular structures constructed from thousands of copies of the protein flagellin, or FliC, arranged in helical fashion. Individual unfolded FliC subunits traverse the filament pore and are folded and sorted into place with the assistance of the flagellar capping protein complex, an oligomer of the FliD protein. The FliD filament cap is a stool-like structure, with its D2 and D3 domains forming a flat head region, and its D1 domain leg-like structures extending perpendicularly from the head towards the inner core of the filament. Here, using an approach combining bacterial genetics, motility assays, electron microscopy and molecular modeling, we define, in numerous Gram-negative bacteria, which regions of FliD are critical for interaction with FliC subunits and result in the formation of functional flagella. Our data indicate that the D1 domain of FliD is its sole functionally important domain, and that its flexible coiled coil region comprised of helices at its extreme N- and C-termini controls compatibility with the FliC filament. FliD sequences from different bacterial species in the head region are well tolerated. Additionally, head domains can be replaced by small peptides and larger head domains from different species and still produce functional flagella.
In Vivo Experimental Endovascular Uses of Cyanoacrylate in Non-Modified Arteries: A Systematic Review
Authors: Guillen Kévin , Comby Pierre-Olivier , Chevallier Olivier , Salsac Anne-Virginie , Loffroy Romaric
Published: September 2021

Cyanoacrylates were first used for medical purposes during World War II to close skin wounds. Over time, medical applications were developed, specifically in the vascular field. Uses now range from extravascular instillation in vascular grafting to intravascular injection for embolization. These applications were made possible by the conduct of numerous preclinical studies involving a variety of tests and outcome measures, including angiographic and histological criteria. Cyanoacrylates were first harshly criticized by vascular surgeons, chiefly due to their fast and irreversible polymerization. Over the past five years, however, cyanoacrylates have earned an established place in endovascular interventional radiology. Given the irreversible effects of cyanoacrylates, studies in animal models are ethically acceptable only if supported by reliable preliminary data. Many animal studies of cyanoacrylates involved the experimental creation of aneurysms or arteriovenous fistulas, whose treatment by endovascular embolization was then assessed. In clinical practice, however, injection into non-modified arteries may be desirable, for instance, to deprive a tumor of its vascular supply. To help investigators in this field select the animal models and procedures that are most appropriate for their objectives, we have reviewed all published in vivo animal studies that involved the injection of cyanoacrylates into non-modified arteries to discuss their main characteristics and endpoints.
The Role of Vitamins in Neurodegenerative Disease: An Update
Authors: Rai Sachchida Nand , Singh Payal , Steinbusch Harry W.M. , Vamanu Emanuel , Ashraf Ghulam , Singh Mohan Prasad
Published: September 2021

Acquiring the recommended daily allowance of vitamins is crucial for maintaining homeostatic balance in humans and other animals. A deficiency in or dysregulation of vitamins adversely affects the neuronal metabolism, which may lead to neurodegenerative diseases. In this article, we discuss how novel vitamin-based approaches aid in attenuating abnormal neuronal functioning in neurodegeneration-based brain diseases such as Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, Amyotrophic lateral sclerosis, and Prion disease. Vitamins show their therapeutic activity in Parkinson’s disease by antioxidative and anti-inflammatory activity. In addition, different water- and lipid-soluble vitamins have also prevented amyloid beta and tau pathology. On the other hand, some results also show no correlation between vitamin action and the prevention of neurodegenerative diseases. Some vitamins also exhibit toxic activity too. This review discusses both the beneficial and null effects of vitamin supplementation for neurological disorders. The detailed mechanism of action of both water- and lipid-soluble vitamins is addressed in the manuscript. Hormesis is also an essential factor that is very helpful to determine the effective dose of vitamins. PubMed, Google Scholar, Web of Science, and Scopus were employed to conduct the literature search of original articles, review articles, and meta-analyses.
Heart-Ankle Pulse Wave Velocity Is Superior to Brachial-Ankle Pulse Wave Velocity in Detecting Aldosterone-Induced Arterial Stiffness
Authors: Chen Zheng-Wei , Pan Chien-Ting , Tsai Cheng-Hsuan , Chang Yi-Yao , Chang Chin-Chen , Lee Bo-Ching , Chiu Yu-Wei , Huang Wei-Chieh , Lin Yu-Li , Wu Vin-Cent , Hung Chi-Sheng , Liao Che-Wei , Lin Yen-Hung ,   on behalf of TAIPAI Study Group
Published: September 2021

Primary aldosteronism (PA) is associated with higher arterial stiffness compared to essential hypertension (EH). However, few studies have compared different pulse wave velocity (PWV) parameters to detect aldosterone-induced arterial stiffness. In this study, we aimed to compare the sensitivity in detecting aldosterone-induced arterial stiffness between brachial-ankle PWV (baPWV) and heart-ankle PWV (haPWV). We prospectively enrolled 1006 PA patients and 983 EH patients. Detailed medical history, basic biochemistry data and two PWV measurements (baPWV and haPWV) were collected in both groups. We performed analysis on the original cohort and two propensity score matching (PSM) models (model 1 adjusted for age and sex; model 2 adjusted for age, sex, systolic and diastolic blood pressure). The DeLong test was used to compare areas under receiver operating characteristic curves (AUCs) between baPWV and haPWV to predict PA. In all models, the PA patients had significantly higher baPWV compared to the EH patients. The AUC of haPWV was greater than that of baPWV. In conclusion, haPWV seems to be a better PWV parameter than baPWV in detecting aldosterone-induced arterial stiffness.
Impact of Viral Decontamination Method on Cytokine Profile of COVID-19 Patients
Authors: Magrì Davide , Navarro Anna , Bergami Federica , Percivalle Elena , Ferrari Alessandro , Lettieri Teresa , Calzolai Luigi , Piralla Antonio , Baldanti Fausto , Gioria Sabrina
Published: September 2021

COVID-19 related morbidity and mortality have been often attributed to an exaggerated immune response. The role of cytokines and chemokines in COVID-19 and their contributions to illness severity are known, and thus their profiling from patient bronchoalveolar lavage (BAL) samples would help in understanding the disease progression. To date, limited studies have been performed on COVID-19 BAL samples, as the manipulation of such specimens (potentially containing live viruses) requires several laboratorial precautions, such as personnel training and special equipment, a requirement that not all laboratories can fulfil. Here, we assessed two fast and easily applicable methods (ultrafiltration and ultraviolet–C irradiation) for their impact on viral load removal or inactivation, respectively and on cytokine profiles preservation. Eight samples of BAL fluids from SARS-CoV2 patients with high viral load were tested. For both methods, complete removal was confirmed by lack of viral replication in Vero E6 cells and by RT-qPCR. Although both methods showed to remove completely the active SARS-CoV2 viral load, only UVC treatment has little or no quantitative effect on total cytokines/chemokines measurements, however cytokines profile and relative ratios are preserved or minimally altered when compared data obtained by the two different decontamination methods. Sample preparation and manipulation can greatly affect the analytical results; therefore, understanding if changes occurred after sample processing is of outmost importance for reliable data and can be useful to improve clinical practice.
Has the Phase of the Menstrual Cycle Been Considered in Studies Investigating Pressure Pain Sensitivity in Migraine and Tension-Type Headache: A Scoping Review
Authors: Curiel-Montero Francisca , Alburquerque-Sendín Francisco , Fernández-de-las-Peñas César , Rodrigues-de-Souza Daiana P.
Published: September 2021

Objective: The aim of this scoping review was to identify if the phase of the menstrual cycle was considered in observational studies comparing pressure pain sensitivity between women with migraine or tension-type headache (TTH) and headache-free women. Methods: A systematic electronic literature search in PubMed, Medline, Web of Science, Scopus, and CINAHL databases was conducted. Observational studies including one or more groups with TTH and/or migraine comparing pressure pain thresholds (PPTs) were included. The methodological quality (risk of bias) was assessed with the Newcastle-Ottawa Scale. Authors, objectives, inclusion/exclusion criteria, size sample, female sample, tool to assess PPTs, mean age, and the use of any medication were extracted and analyzed independently by two authors. Results: From a total of 1404 and 1832 identified articles for TTH and migraine, 30 and 18 studies satisfied the criteria and were included. Nineteen (63.4%) studies assessing TTH patients and eleven (61.1%) assessing migraine patients showed a high risk of bias. The most common flaws were attributed to improper selection of control and control over other additional factors. Based on the systematic review, just one study including TTH and one including migraine patients considered the menstrual cycle. Conclusion: The results of this scoping review identified that the phase of the menstrual cycle has been rarely considered in studies investigating sensitivity to pressure pain in primary headaches, such as TTH or migraine, although there is evidence showing the relevance of the phase of the menstrual cycle in pain perception.
Nucleus Accumbens Chemogenetic Inhibition Suppresses Amphetamine-Induced Ultrasonic Vocalizations in Male and Female Rats
Authors: Lawson Kate A. , Flores Abigail Y. , Hokenson Rachael E. , Ruiz Christina M. , Mahler Stephen V.
Published: September 2021

Adult rats emit ultrasonic vocalizations (USVs) related to their affective states, potentially providing information about their subjective experiences during behavioral neuroscience experiments. If so, USVs might provide an important link between invasive animal preclinical studies and human studies in which subjective states can be readily queried. Here, we induced USVs in male and female Long Evans rats using acute amphetamine (2 mg/kg), and asked how reversibly inhibiting nucleus accumbens neurons using designer receptors exclusively activated by designer drugs (DREADDs) impacts USV production. We analyzed USV characteristics using “Deepsqueak” software, and manually categorized detected calls into four previously defined subtypes. We found that systemic administration of the DREADD agonist clozapine-n-oxide, relative to vehicle in the same rats, suppressed the number of frequency-modulated and trill-containing USVs without impacting high frequency, unmodulated (flat) USVs, nor the small number of low-frequency USVs observed. Using chemogenetics, these results thus confirm that nucleus accumbens neurons are essential for production of amphetamine-induced frequency-modulated USVs. They also support the premise of further investigating the characteristics and subcategories of these calls as a window into the subjective effects of neural manipulations, with potential future clinical applications.
Development of Double-Sided Multipoint Press CNC and Operational Technology for Producing Freeform Molds
Authors: Yun Jiyeong , Youn Jongyoung , Lee Donghoon
Published: September 2021

Many studies concerning the precision manufacturing of freeform concrete panels have been conducted, however, this process remains labor intensive taking significant amounts of time and cost. In particular, the precision in the shape of the panels produced tends to be low because of the manual work involved in producing the curves of those panels. This study documents the development of mold production technologies that can be used to produce precise curved surfaces on the upper and lower parts of a mold for freeform concrete panels. A double-sided multipoint press CNC (computer numerical control) produces curved upper and lower surfaces of a mold without the need for manual work, while the operational technology we developed to control this tool enhances the precision of the curves created. The precision of these technologies was verified through experiments. The difference between the shapes designed and those produced were found using 3D scans and quality inspections. Unpredictable errors can occur due to the size of certain curvatures, the elasticity of the silicone plate, and the rotational angle of the joints of the rods supporting the surfaces. To minimize errors, shape compensation was carried out through reverse engineering, leading to a maximum error of 2.887 mm, which is within the allowable error. The results achieved in this study are a significant step toward technologies that will produce increasingly precise freeform concrete panels.
Chronic Obstructive Pulmonary Disease and Its Acute Exacerbation before Colon Adenocarcinoma Treatment Are Associated with Higher Mortality: A Propensity Score-Matched, Nationwide, Population-Based Cohort Study
Authors: Chen Yen-Chang , Li Ming-Chang , Yu Ying-Hui , Lin Chih-Ming , Wu Szu-Yuan
Published: September 2021

Purpose: To investigate whether chronic obstructive pulmonary disease (COPD) and COPD severity (acute exacerbation of COPD (AECOPD)) affect the survival outcomes of patients with colon adenocarcinoma receiving standard treatments. Methods: From the Taiwan Cancer Registry Database, we recruited patients with clinical stage I–III colon adenocarcinoma who had received surgery. The Cox proportional hazards model was used to analyze all-cause mortality. We categorized the patients into COPD and non-COPD (Group 1 and 2) groups through propensity score matching. Results: In total, 1512 patients were eligible for further comparative analysis between non-COPD (1008 patients) and COPD (504 patients) cohorts. In the multivariate Cox regression analysis, the adjusted hazard ratio (aHR; 95% confidence interval (CI)) for all-cause mortality for Group 1 compared with Group 2 was 1.17 (1.03, 1.29). In patients with colon adenocarcinoma undergoing curative resection, the aHRs (95% CIs) for all-cause mortality in patients with hospitalization frequencies of ≥1 and ≥2 times for AECOPD within 1 year before adenocarcinoma diagnosis were 1.08 (1.03, 1.51) and 1.55 (1.15, 2.09), respectively, compared with those without AECOPD. Conclusion: In patients with colon adenocarcinoma undergoing curative resection, COPD was associated with worse survival outcomes. Being hospitalized at least once for AECOPD within 1 year before colon adenocarcinoma diagnosis was an independent risk factor for poor overall survival in these patients, and a higher number of hospitalizations for AECOPD within 1 year before diagnosis was associated with poorer survival. Our study highlights the importance of COPD management, particularly the identification of frequent exacerbators and the prevention of AECOPD before standard colon adenocarcinoma treatments are applied.
A Novel Human Biospecimen Repository for Clinical and Molecular Investigation of Thoracic Aortopathy
Authors: Vujakovich Courtney E. , Landis Benjamin J.
Published: September 2021

Thoracic aortic aneurysm (TAA) is a heritable aortopathy with significant morbidity and mortality, affecting children and adults. Genetic causes, pathobiological mechanisms, and prognostic markers are incompletely understood. In 2015, the Collaborative Human Aortopathy Repository (CHAR) was created to address these fundamental gaps. Patients with thoracic aortopathy, associated genetic diagnoses, or aortic valve disease are eligible for prospective enrollment. Family members and controls are also enrolled. Detailed clinical and family data are collected, and blood and aortic tissue biospecimens are processed for broad usage. A total of 1047 participants were enrolled. The mean age in 834 affected participants was 47 ± 22 (range <1 to 88) years and 580 were male (70%). A total of 156 (19%) were under the age of 21 years. Connective tissue diagnoses such as Marfan syndrome were present in 123 (15%). Unaffected participants included relatives (N = 176) and healthy aorta tissue controls (N = 37). Aortic or aortic valve biospecimens were acquired from over 290 and 110 participants, respectively. RNA and protein were extracted from cultured aortic smooth muscle cells (SMCs) for 90 participants. Over 1000 aliquots of aortic SMCs were cryopreserved. The CHAR’s breadth, robust biospecimen processing, and phenotyping create a unique, multipronged resource to accelerate our understanding of human aortopathy.
Organocatalytic Asymmetric Michael Addition in Aqueous Media by a Hydrogen-Bonding Catalyst and Application for Inhibitors of GABAB Receptor
Authors: Shim Jae Ho , Hong Yeonsun , Kim Ji Hae , Kim Hyeon Soo , Ha Deok-Chan
Published: September 2021

Catalysts based on (R, R)-1,2-diphenylethylenediamine are, as chiral organic catalysts, applied to the asymmetric Michael addition to α, β-unsaturated nitroalkenes under neutral conditions. The role of an aqueous medium for organic catalytic activity can be reversed concerning hydrophilic-hydrophobic function depending on the reaction conditions. In this study, to provide an environmentally friendly system, the thiourea-based catalyst substituted with 3,5-(CF3)2-Ph was used in water solvents. The hydrophobic effect of the substituent provided fast reaction, high chemical yield, and mirror-image selectivity. This reaction allowed the preparation of GABAB agonists in an optically pure manner. Additionally, GABA (γ-aminobutyric acid) analogs such as baclofen and phenibut were synthesized as R-type S-type with high optical purity.
Atoms vs. Ions: Intermediates in Reversible Electrochemical Hydrogen Evolution Reaction
Authors: Juodkazytė Jurga , Juodkazis Kȩstutis , Juodkazis Saulius
Published: September 2021

We present a critical analysis of the mechanism of reversible hydrogen evolution reaction based on thermodynamics of hydrogen processes considering atomic and ionic species as intermediates. Clear distinction between molecular hydrogen evolution/oxidation (H2ER and H2OR) and atomic hydrogen evolution/oxidation (HER and HOR) reactions is made. It is suggested that the main reaction describing reversible H2ER and H2OR in acidic and basic solutions is: H3O++2e−⇌(H2+)adH2+OH− and its standard potential is E0 = −0.413 V (vs. standard hydrogen electrode, SHE). We analyse experimentally reported data with models which provide a quantitative match (R.J.Kriek et al., Electrochem. Sci. Adv. e2100041 (2021)). Presented analysis implies that reversible H2 evolution is a two-electron transfer process which proceeds via the stage of adsorbed hydrogen molecular ion H2+ as intermediate, rather than Had as postulated in the Volmer-Heyrovsky-Tafel mechanism. We demonstrate that in theory, two slopes of potential vs. lg(current) plots are feasible in the discussed reversible region of H2 evolution: 2.3RT/F≈60 mV and 2.3RT/2F≈30 mV, which is corroborated by the results of electrocatalytic hydrogen evolution studies reported in the literature. Upon transition to irreversible H2ER, slowdown of H2+ formation in the first electron transfer stage manifests, and the slope increases to 2.3RT/0.5F≈120 mV; R,F,T are the universal gas, Faraday constants and absolute temperature, respectively.
DNA Fingerprint Analysis of Raman Spectra Captures Global Genomic Alterations in Imatinib-Resistant Chronic Myeloid Leukemia: A Potential Single Assay for Screening Imatinib Resistance
Authors: Mojidra Rahul , Hole Arti , Iwasaki Keita , Noothalapati Hemanth , Yamamoto Tatsuyuki , C Murali Krishna , Govekar Rukmini
Published: September 2021

Monitoring the development of resistance to the tyrosine kinase inhibitor (TKI) imatinib in chronic myeloid leukemia (CML) patients in the initial chronic phase (CP) is crucial for limiting the progression of unresponsive patients to terminal phase of blast crisis (BC). This study for the first time demonstrates the potential of Raman spectroscopy to sense the resistant phenotype. Currently recommended resistance screening strategy include detection of BCR-ABL1 transcripts, kinase domain mutations, complex chromosomal abnormalities and BCR-ABL1 gene amplification. The techniques used for these tests are expensive, technologically demanding and have limited availability in resource-poor countries. In India, this could be a reason for more patients reporting to clinics with advanced disease. A single method which can identify resistant cells irrespective of the underlying mechanism would be a practical screening strategy. During our analysis of imatinib-sensitive and -resistant K562 cells, by array comparative genomic hybridization (aCGH), copy number variations specific to resistant cells were detected. aCGH is technologically demanding, expensive and therefore not suitable to serve as a single economic test. We therefore explored whether DNA finger-print analysis of Raman hyperspectral data could capture these alterations in the genome, and demonstrated that it could indeed segregate imatinib-sensitive and -resistant cells. Raman spectroscopy, due to availability of portable instruments, ease of spectrum acquisition and possibility of centralized analysis of transmitted data, qualifies as a preliminary screening tool in resource-poor countries for imatinib resistance in CML. This study provides a proof of principle for a single assay for monitoring resistance to imatinib, available for scrutiny in clinics.
Influence of Isovalent ‘W’ Substitutions on the Structure and Electrical Properties of La2Mo2O9 Electrolyte for Intermediate-Temperature Solid Oxide Fuel Cells
Authors: Paul Tanmoy , Tsur Yoed
Published: September 2021

Lanthanum molybdenum oxide (La2Mo2O9, LAMOX)-based ion conductors have been used as potential electrolytes for solid oxide fuel cells. The parent compound La2Mo2O9 undergoes a structural phase transition from monoclinic (P21) to cubic (P213) at 580 °C, with an enhancement in oxide ion conductivity. The cubic phase is of interest because it is beneficial for oxide ion conduction. In search of alternative candidates with a similar structure that might have a stable cubic phase at lower temperatures, we have studied the variations of the crystal structure and ionic conductivity for 25, 50, 62.5 and 75 mol% W substitutions at the Mo site using high-temperature X-ray diffraction, dilatometry, and impedance spectroscopy. Highly dense ceramic samples have been synthesized by solid-state reaction in a two-step sintering process. Low-angle X-ray diffraction and Rietveld refinement confirm the stabilization of the cubic phase for all compounds in the entire temperature range considered. The substitutions of W at the Mo site produce a decrement in the lattice parameter. The thermal expansion coefficients in the high-temperature range of the W-substituted ceramics, as determined by dilatometry, are much higher than that of the unmodified sample. The impedance spectra have been modeled using a modified genetic algorithm within 300–600 °C. A distribution function of the relaxation times is obtained, and the contributions of ohmic drop, grains and grain boundaries to the conductivity have been identified. Overall, our investigation provides information about cationic substitution and insights into the understanding of oxide ion conductivity in LAMOX-based compounds for developing solid oxide fuel cells.
Feasibility and Safety of Percutaneous Cardiac Interventions for Congenital and Acquired Heart Defects in Infants ≤1000 g
Authors: Philip Ranjit , Towbin Jeffrey , Tailor Neil , Joshi Vijaya , Johnson Jason N. , Naik Ronak , Waller B. Rush , Sathanandam Shyam
Published: September 2021

The transcatheter closure of patent ductus arteriosus (TCPC) has been demonstrated to be feasible even in infants weighing ≤1000 g. However, other percutaneous cardiac interventions (PCI) for such small infants born with congenital heart defects (CHD) or acquired heart defects (AHD) have not been well described. The purpose of this study was to describe the feasibility and safety of PCI in infants ≤1000 g. A retrospective review was conducted between June 2015 and May 2021, looking at 148 consecutive PCIs performed on infants weighing ≤1000 g at the time of the procedure. The procedural success rate was 100%. The major adverse event (AE) rate for TCPC was 3%, while there were no major AEs for other PCI. It is feasible to perform PCIs in infants weighing ≤1000 g with CHD and AHD using currently available technologies.
Two Sides of a Coin: Parental Disease-Specific Training as Seen by Health Care Practitioners and Parents in Pediatric Liver Transplantation
Authors: Ohlendorf Johanna , Stasch Luisa , Baumann Ulrich , Konietzny Christiane , Pfister Eva-Doreen , Ernst Gundula , Lange Karin , Sautmann Kirsten , Goldschmidt Imeke
Published: September 2021

In the absence of widely accepted education standards for parents of children after liver transplantation (LTx), the content and structure of parental training are influenced by health care practitioners’ (HCP) individual knowledge and assessment of the relevance of its contents. This study examines the hypothesis that expectations towards training differ between HCPs and parents, and that the quality of parental training affects the job-satisfaction of HCPs. Attitudes towards disease-specific education were assessed by tailor-made questionnaires in HCPs (n = 20) and parents of children with chronic liver disease or after LTx (n = 113). These were supplemented by focused interviews in n = 7 HCPs and n = 16 parents. Parents were more satisfied with current counseling than HCP. Language barriers and low parental educational background were perceived as obstacles by 43% of HCPs. The quality of parental knowledge was felt to have a strong influence on HCPs job satisfaction. The expectations towards the content of disease-specific education largely overlap but are not synonymous. HCP and parents agreed with regards to the importance of medication knowledge. Parents rated the importance about the meaning of laboratory values and diagnostic procedures significantly higher (3.50 vs. 2.85, p < 0.001 and 3.42 vs. 2.80, p < 0.001) than HCPs. Parents and HCPs would prefer a structured framework with sufficient staff resources for disease-specific counseling.
Mechanisms and Insights for the Development of Heart Failure Associated with Cancer Therapy
Authors: Fraley Claire , Milgrom Sarah A. , Kondapalli Lavanya , Taylor Matthew R. G. , Mestroni Luisa , Miyamoto Shelley D.
Published: September 2021

Cardiotoxicity is a well-recognized late effect among childhood cancer survivors. With various pediatric cancers becoming increasingly curable, it is imperative to understand the disease burdens that survivors may face in the future. In order to prevent or mitigate cardiovascular complications, we must first understand the mechanistic underpinnings. This review will examine the underlying mechanisms of cardiotoxicity that arise from traditional antineoplastic chemotherapies, radiation therapy, hematopoietic stem cell transplantation, as well as newer cellular therapies and targeted cancer therapies. We will then propose areas for prevention, primarily drawing from the anthracycline-induced cardiotoxicity literature. Finally, we will explore the role of human induced pluripotent stem cell cardiomyocytes and genetics in advancing the field of cardio-oncology.
Topical Probiotics Do Not Satisfy New Criteria for Effective Use Due to Insufficient Skin Microbiome Knowledge
Authors: Wallen-Russell Christopher , Wallen-Russell Samuel
Published: September 2021

We propose a set of criteria for topical probiotics to adhere to for safe and effective use for the skin microbiome. To form the basis of the criteria, we redefine the term “probiotics” and discuss successful and unsuccessful high-profile examples of the artificial addition of organisms to ecosystems in nature to understand what worked and what did not. Probiotics are often immediately assumed to have health benefits. However, as ecologists are aware, interfering with ecosystems is potentially catastrophic. The addition or removal of just one organism can significantly upset the delicate ecosystem balance. If our criteria are not met, we argue that topical probiotics could also cause damage and will not be beneficial. Due to the large intra- and inter-personal variation of the skin microbiome, our current knowledge of a healthy skin microbiome composition is not complete enough to fully satisfy the criteria. In follow-up work, we will investigate whether current topical probiotics research and commercial products meet our new criteria. We will also discuss problems with how to measure their effectiveness and suggest alternative solutions to replacing the lost biodiversity of the skin microbiome that was stripped away by environmental factors in the Western world.
The Cost of a True Random Bit—On the Electronic Cost Gain of ASIC Time-Domain-Based TRNGs
Authors: Klein Netanel , Harel Eyal , Levi Itamar
Published: September 2021

Random number generators are of paramount importance in numerous fields. Under certain well-defined adversarial settings, True Random Number Generators (TRNGs) are more secure than their computational (pseudo) random number generator counterparts. TRNGs are also known to be more efficiently implemented on hardware platforms where, for various applications, efficiency in terms of electronic cost factors is critical. In this manuscript, we first provide an evaluation of robustness and reliability of efficient time-domain-based TRNG implementation over FPGA platform. In particular, we demonstrate sensitivities which imply a TRNG construction which is not agnostic to electronic-design-automation tools and to the level of designers’ know-how. This entails a large amount of effort and validation to make the designs robust, as well as requires a high degree of complexity from non-trivial FPGAs flows. This motivates the second part of the manuscript, where we propose an ASIC-based implementation of the TRNG, along with the optimization steps to enhance its characteristics. The optimized design improves the randomness-throughput by 42× for the same entropy level described in previous works, and it can provide maximal entropy level of 0.985 with 7× improvement in randomness throughput over the raw samples (no pre-processing). The proposed design simultaneously provides a reduced energy of 0.1 (mW/bit) for the same entropy level as previous works, and 1.06 (mW/bit) for the higher entropy flavor, and a lower area utilization of 0.000252 (mm2) on a 65 nm technology evaluation, situating it in the top-class of the discuss ratings. This leads to the quantitative question of the gain in electronic cost factors over ASIC TRNGs, and the minimum Cost Per Bit/Source possible to date. Finally, we exemplify a TRNG versus PRNG cost-extrapolation for security architects and designers, targeting an ASIC scenario feeding a lightweight encryption core.
Eccrine Nevus in the Forearm of a 16-Year-Old Presenting as Unilateral Hyperhidrosis: A Clinicopathological Correlation Paradigm
Authors: Martin-Gorgojo Alejandro , Sanchez-Carpintero Ignacio , Ruiz-Rodriguez Ricardo , Enguita-Valls Ana-Belen
Published: September 2021

A case of a purely eccrine nevus in an adolescent patient presenting with focal hyperhidrosis on an area comprising the left forearm and the dorsal aspect of the left hand is described. No clinically evident lesions were identifiable. Dermatopathologic findings were subtle, showing only a slight increase in the number of eccrine glands. Clinicopathological correlation was paramount to achieve the diagnosis.
Leveraging AI to Estimate Caribou Lichen in UAV Orthomosaics from Ground Photo Datasets
Authors: Richardson Galen , Leblanc Sylvain G. , Lovitt Julie , Rajaratnam Krishan , Chen Wenjun
Published: September 2021

Relating ground photographs to UAV orthomosaics is a key linkage required for accurate multi-scaled lichen mapping. Conventional methods of multi-scaled lichen mapping, such as random forest models and convolutional neural networks, heavily rely on pixel DN values for classification. However, the limited spectral range of ground photos requires additional characteristics to differentiate lichen from spectrally similar objects, such as bright logs. By applying a neural network to tiles of a UAV orthomosaics, additional characteristics, such as surface texture and spatial patterns, can be used for inferences. Our methodology used a neural network (UAV LiCNN) trained on ground photo mosaics to predict lichen in UAV orthomosaic tiles. The UAV LiCNN achieved mean user and producer accuracies of 85.84% and 92.93%, respectively, in the high lichen class across eight different orthomosaics. We compared the known lichen percentages found in 77 vegetation microplots with the predicted lichen percentage calculated from the UAV LiCNN, resulting in a R2 relationship of 0.6910. This research shows that AI models trained on ground photographs effectively classify lichen in UAV orthomosaics. Limiting factors include the misclassification of spectrally similar objects to lichen in the RGB bands and dark shadows cast by vegetation.