bluuebunny/crossref_metadata_2025 huggingface dataset:
Bone Milling: On Monitoring Cutting State and Force Using Sound Signals
Authors: Ying Zhenzhi , Shu Liming , Sugita Naohiko
Published: May 2022

Efficient monitoring of bone milling conditions in orthopedic and neurosurgical surgery can prevent tissue, bone, and tool damage, and reduce surgery time. Current researches are mainly focused on recognizing the cutting state using force signal. However, the force signal during the milling process is difficult and expensive to acquire. In this study, a neural network-based method is proposed to recognize the cutting state and force during the bone milling process using sound signals. Numerical modeling of the cutting force is performed to capture the relationship between the cutting force and the depth of cut in the bone milling process. The force model is used to calibrate the training data to improve the recognition accuracy. Wavelet package transform is used for signal processing to understand bone-cutting phenomena using sound signals. The proposed system succeeds to monitor the bone milling process to reduce the surgical risk. Experiments on standard bone specimens and vertebrae also indicate that the proposed approach has considerable potential for use in computer-assisted and robot-assisted bone-cutting systems used in various types of surgery.
The effect of nafamostat mesilate infusion after ERCP for post-ERCP pancreatitis
Authors: Kim Joo Seong , Lee Sang Hyub , Park Namyoung , Huh Gunn , Chun Jung Won , Choi Jin Ho , Cho In Rae , Paik Woo Hyun , Ryu Ji Kon , Kim Yong-Tae
Published: May 2022

Background

Nafamostat mesilate decreases the incidence of pancreatitis after endoscopic retrograde cholangiopancreatography (ERCP). However, no studies have administered nafamostat mesilate after ERCP. So we investigated if the infusion of nafamostat mesilate after ERCP can affect the post-ERCP pancreatitis (PEP) in high-risk patients.

Methods

In a tertiary hospital, 350 high-risk patients of PEP were reviewed retrospectively. Among them, 201 patients received nafamostat mesilate after ERCP. Patient-related and procedure-related risk factors for PEP were collected. We performed a propensity score matching to adjust for the significant different baseline characteristics. The incidence and severity of PEP were evaluated according to the infusion of nafamostat mesilate. The risk factors of PEP were also analyzed by multivariate logistic regression.

Results

The baseline characteristics were not different after the matching. The PEP rate (17.4% vs. 10.3%, P = 0.141) was insignificant. Among the PEP patients, mild PEP was significantly higher in the nafamostat mesilate group (85.7% vs. 45.5%, P = 0.021). Only one patient in the nafamostat mesilate group developed severe PEP. Although young age (odds ratio [OR] 3.60, 95% CI 1.09–11.85, P = 0.035) was a risk factor, nafamostat mesilate (odds ratio [OR] 0.30, 95% CI 0.09–0.98, P = 0.047) was a protective factor for moderate to severe PEP.

Conclusions

The administration of nafamostat mesilate after ERCP in high-risk patients was not effective in preventing PEP, but may attenuate the severity of PEP.
Discordant results of Xpert MTB/Rif assay and BACTEC MGIT 960 liquid culture to detect Mycobacterium tuberculosis in community screening in Vietnam
Authors: Nguyen Hai Viet , de Haas Petra , Nguyen Hoa Binh , Nguyen Nhung Viet , Cobelens Frank G. J. , Mirtskhulava Veriko , Finlay Alyssa , Van Nguyen Hung , Huyen Pham T. T. , Tiemersma Edine W.
Published: May 2022

Background

Xpert MTB/Rif, a molecular test to detect tuberculosis (TB), has been proven to have high sensitivity and specificity when compared with liquid culture in clinical settings. However, little is known about its performance in community TB screening.

Methods

In Vietnam, a national TB prevalence survey was conducted in 2017. Survey participants who screened positive by chest X-ray, cough symptoms and/or recent history of tuberculosis were requested to provide at least two sputum samples that were tested for Mycobacterium tuberculosis by Xpert MTB/Rif G4 (Xpert) and BACTEC MGIT960 culture (MGIT).

Results

There were 4,649 eligible participants provided both samples for testing. Among them, 236 (5.1%) participants tested positive for TB by Xpert, 244 (5.3%) tested positive by MGIT and 317 tested positive by at least one test; 163 (51.4%) had discordant test results. Of the positive Xpert, 162 (68.6%) showed a low or very low bacterial load. In multivariate logistic regression comparing discordant with Xpert-MGIT concordant positive results, discordant Xpert-positive results occurred more often among participants who had low sputum bacterial load, male sex, a history of TB treatment, or night sweats. The associated factors were male sex, abnormal chest X-ray and having night sweats when the logistic model was against those with both Xpert and MGIT negative.

Conclusions

We found high rates of discordance in the performance of Xpert and MGIT for community-based TB case finding. In situations where the majority of TB cases are expected to have a low bacterial load, multiple diagnostic tests and/or multiple samples are required to reach sufficient sensitivity.
Prognostic values of left ventricular mass index progression in incident peritoneal dialysis patients : a prospective cohort study
Authors: Chen Yun , Dai Shuqi , Ge Xiaolin , Shang Da , Xie Qionghong , Hao Chuanming , Zhu Tongying
Published: May 2022

Background

Left ventricular hypertrophy (LVH) is common among patients undergoing dialysis. However, the dynamic structural changes of LV are rarely discussed. The study aimed to investigate the prognostic significance of left ventricular mass index (LVMI)-progression in incident peritoneal dialysis (PD) patients, and explore risks factors for LVMI-progression.

Methods

Incident PD patients between February 2008 and July 2018 were recruited. Echocardiography was performed yearly to collect LVMI and evaluate its changes. Participants were divided into three subgroups: group with LVMI-regression, group with LVMI stable and group with LVMI-progression. The end points include all-cause mortality, cardiovascular mortality and cardiovascular events. Cox regression models were performed to identify the associations between LVMI-progression and these endpoints. Multivariate logistic regression was conducted to identify risk factors for LVMI-progression.

Results

A total of 216 PD patients (130 men,60.2%) with a mean age of 54.3 ± 16.8 years were recruited. LVMI-progression was identified in 72 patients (33.3%) after PD initiation. The cohort was followed for a median duration of 65.9 months. Multivariable Cox regression analysis revealed that LVMI-progression was an independent predictor of all-cause mortality (HR, 1.419; 95% CI, 1.016–1.982; p = 0.040), cardiovascular mortality (HR, 1.836; 95%CI, 1.084–3.108; p = 0.024), and cardiovascular events (HR, 1.494; 95%CI, 1.063–2.099; p = 0.021). Multivariable logistic regression showed that hemoglobin, ferritin, blood pressure and fibrinogen were significantly associated with LVMI-progression.

Conclusion

Early LVMI-progression was independently associated with all-cause mortality and cardiovascular outcomes in PD patients. The dynamic monitoring of LVMI might therefore help identify high-risk patients.
Adolescent pregnancy in Sao Tome and Principe: are there different obstetric and perinatal outcomes?
Authors: Vasconcelos Alexandra , Bandeira Nelson , Sousa Swasilanne , Machado Maria Céu , Pereira Filomena
Published: May 2022

Background

Adolescent childbirth is a major public health problem in Sao Tome and Principe (STP). Adolescent pregnancy and childbirth can carry a risk of morbidity associated with the physiological and sociological characteristics of teenage girls. This study aims to identify the main adverse obstetric and perinatal outcomes for adolescent pregnancies in the Hospital Dr. Ayres de Menezes (HAM), the only hospital in STP.

Methods

An institution-based cross-sectional study. Pregnant women ≤ 19 years of age (n = 104) were compared to non-adolescent women (n = 414). The obstetric and perinatal outcomes were compared between groups using the t test. Odds ratio (OR) were calculated through Cochran’s and Mantel–Haenszel statistics test for odds ratio equal to 1, 95% confidence intervals (CI) and p values (p < 0.05) were considered significant.

Results

The adverse perinatal outcomes imputable to adolescent births were foetal distress with low first minute Apgar score < 7 (OR 1.94, 95% CI 1.18–3.18, p = 0.009) and performance of neonatal resuscitation manoeuvres (OR 2.4, 95% CI 1.07–5.38, p = 0.032). Compared to older mothers, teenage girls were likely to have a non-statistically significant threefold higher risk of having an obstructed labour (OR 3.40, 95% CI 0.89–12.94, p = 0.07). Other perinatal outcomes as neonatal asphyxia, risk for cerebral palsy, premature birth, early neonatal infection, and neonatal death were identical between groups as well as maternal anaemia, mode of delivery or other obstetrical outcomes.

Conclusion

Adolescent pregnancies were associated with worse perinatal outcomes as foetal distress and higher need for neonatal resuscitation manoeuvres. This study may support STP health authorities in their efforts to make Sustainable Development Goals 3 (good health and wellbeing), 4 (quality education) and 5 (gender equality) a reality by 2030, since it identifies specific problems that need to be addressed to improve maternal adolescent health.
Predictors of central and general obesity in Iranian preschool children: which anthropometric indices can be used as screening tools?
Authors: Mardali Farzaneh , Naziri Mahdyieh , Sohouli Mohammad Hassan , Fatahi Somaye , Sadat Hosseini-Baharanchi Fatemeh , Găman Mihnea-Alexandru , Shidfar Farzad
Published: May 2022

Aim

To compare the ability of anthropometric indices [waist-to-hip ratio (WHR), waist-to-height ratio (WHtR), neck-to-height ratio (NHR), conicity index (CI), body adiposity index (BAI), tri-ponderal mass index (TMI) and body mass index (BMI)] and,measuerments like neck(NC), hip(HC) and waist circumferences to predict overweight and obesity in Iranian preschool children.

Materials and Methods

A total of 498 Iranian preschool children were included in this case–control study conducted in Tehran, Iran. The participants were selected using the stratified random sampling procedure based on gender and school. Using sex-based receiver operating curve (ROC) analysis, we compared the area under the curve and defined the cut-off points for detecting central and general obesity for each index in order to identify the most suitable tools in predicting obesity.

Results

Boys had significantly higher values for NC, WC, WHR, NHR, CI, TMI and BMI as compared to girls, whereas BAI and HC were higher in girls. The area under the curve was calculated for all the possible predictors of central obesity, i.e., NC (0.841–0.860), WC (0.70–0.679), HC (0.785–0.697), WHR (0.446–0.639) and CI (0.773–0.653) in boys and girls, respectively. And according to the ROC curve analysis, BMI (0.959–0.948), TMI (0.988–0.981), WHtR (0.667–0.553) and NHR (0.785–0.769) were predictors of general obesity and NC (0.841–0.860) as predictor of central obesity in boys and girls, respectively. The optimal cut-off points for TMI (13.80–15.83), NC (28.68–27.5) and for other anthropometric indices were estimated in both boys and girls.

Conclusion

TMI and NC seem to predict general and central obesity in Iranian preschool children.
Factors relevant to work participation from the perspective of adults with developmental dyslexia: a systematic review of qualitative studies
Authors: de Beer Joost , Heerkens Yvonne , Engels Josephine , van der Klink Jac
Published: May 2022

Background

This review is focused on workers with developmental dyslexia (DD). In this review DD is considered an expression of neurodiversity, a consequence of a natural variant of the brain. Evidence was synthesized to explore which factors workers with DD consider relevant for their participation in work and whether these factors reflect shifts in the concepts of health and sustainable employability. The factors were classified according to the International Classification of Functioning, Disability and Health (ICF), adapted for occupational health.

Methods

A systematic review of qualitative studies was performed. Two search strings were used to determine the population and the context of work. The factors were classified using a recently proposed rearrangement of the ICF scheme that places participation in a central position and incorporates preliminary lists of work-related environmental factors and personal factors.

Results

Fifty-one factors were found that appeared in 35% or more of the included studies and that were relevant to work participation according to the workers themselves. These factors were dispersed over all ICF categories. In the category Functions and Structures (11 factors), most of the factors had negative connotations. In the category Activities (9 factors), all the factors cause difficulties, except speaking (which is ambiguous). In the category Participation (4 factors), the formal relationships are important for the degree of participation. Overall, more than half of the factors are environmental (18) or personal (9) and they both hinder and facilitate work participation.

Conclusions

The results of this review give an indication for the importance of the biopsychosocial model as a relevant approach for people with a disability in the world of work. This review also adds data for the usefulness of the proposals for the reconsideration of the ICF scheme. The data has not (yet) returned any visible trends revealing that the concept of neurodiversity is common in organizations.
Household food insecurity and unimproved toilet facilities associate with child morbidity: evidence from a cross-sectional study in Bangladesh
Authors: Islam Mohammad Ashraful , Rahman Mahfuzur , Uddin Md. Fakhar , Tariqujjaman Md. , Karmakar Gobinda , Rahman Mohammad Ashikur , Kelly Matthew , Gray Darren , Ahmed Tahmeed , Sarma Haribondhu
Published: May 2022

Background

Despite recent progress in rural economic development and food production, the prevalence of household food insecurity (FI) and use of unimproved toilet facilities are widespread in Bangladesh. Evidence regarding the consequencs of household FI and poor sanitation on child morbidity is scarce. This study aimed to understand the association of FI and unimproved toilet facility with morbidity status of under-5 children in Bangladesh.

Methods

We used data from a cross-sectional survey that was conducted as part of an evaluation of the Maternal, Infant and Young Child Nutrition (MIYCN) Program in 9 districts of Bangladesh. The study population included children aged 6–59 months and their caregivers, identified using a two-stage cluster-sampling procedure. Child morbidity status was the outcome variable, and household FI status and type of toilet used were considered the main exposure variables in this study. We performed logistic regression, calculated adjusted odds ratios (AOR) to assess the association of child morbidity with household FI and unimproved toilet facility after adjusting for potential confounders.

Results

A total of 1,728 households were eligible for this analysis. About 23% of the households were food-insecure, and a large number of households had improved toilet facilities (93.4%). In the multivariable logistic regression model, we found that children in food-insecure households with unimproved toilet facility had 5.88 (AOR: 5.88; 95% CI 2.52, 13.70) times more chance, of being morbid compared to the children of food-secure households with improved toilet facility. A similar association of FI and toilet facilities with each of the morbidity components was observed, including diarrhea (AOR:3.6; 95% CI 1.79, 7.89), fever (AOR:3.47; 95% CI 1.72, 6.99), difficult or fast breathing with cough (AOR:3.88; 95% CI 1.99, 7.59), and difficult or fast breathing with blocked or running nose (AOR:1.29; 95% CI 0.56, 2.95).

Conclusions

Our study shows that household FI and unimproved toilet facility jointly have more deteriorative effects on child morbidity than either of these conditions alone. Therefore, it is recommended to consider these two critical factors while designing a public health intervention for reducing morbidity among under-five children.
An analysis of English national policy approaches to health inequalities: ‘transforming children and young people’s mental health provision’ and its consultation process
Authors: Griffin Naomi , Wistow Jonathan , Fairbrother Hannah , Holding Eleanor , Sirisena Mihirini , Powell Katie , Summerbell Carolyn
Published: May 2022

Background

A national policy for England, published in 2017, entitled ‘Transforming Children and Young People’s Mental Health Provision’ aimed to address the increasing prevalence mental health problems in children and tackle inequalities. In the context of this policy’s implementation as ongoing and the effects of the Covid-19 pandemic, the need for appropriate, timely and ongoing national government commitment is vital.

Methods

A narrative review using a problem representation evaluation [1], we critiqued the policy and related consultation documents using a social determinants of health perspective. We also reviewed wider policy discourses through engaging with stakeholder responses, providing an innovative methodological contribution to scholarship on public health policy and health inequalities.

Results

We found absences and oversights in relation to inequalities (most notably the lack of acknowledgement that mental health can cause inequalities), access, workforce capacity, and the impacts of cuts and austerity on service provision. We suggest these inadequacies may have been avoided if stakeholder responses to the consultation process had been more meaningfully addressed. We illustrate how ‘problems’ are discursively created through the process of policy development, justified using specific types of evidence, and that this process is politically motivated. Local policy makers have a critical role in translating and adapting national policy for their communities but are constrained by absences and oversights in relation to health inequalities.

Conclusions

This narrative review illustrates how policy discourse frames and produces ‘problems’, and how the evidence used is selected and justified politically. This review contributes to the existing transdisciplinary field of knowledge about how using methods from political and social science disciplines can reveal new insights when critiquing and influencing policy approaches to health inequalities.
Identification of icaritin derivative IC2 as an SCD-1 inhibitor with anti-breast cancer properties through induction of cell apoptosis
Authors: Yang Chen , Jin Yi-Yuan , Mei Jie , Hu Die , Jiao Xiaoyu , Che Hui-Lian , Tang Chun-Lei , Zhang Yan , Wu Guo-Sheng
Published: May 2022

Background

Breast cancer is the most common malignancy affecting women, yet effective targets and related candidate compounds for breast cancer treatment are still lacking. The lipogenic enzyme, stearoyl-CoA desaturase-1 (SCD1), has been considered a potential target for breast cancer treatment. Icaritin (ICT), a prenylflavonoid derivative from the Traditional Chinese Medicine Epimedii Herba, has been reported to exert anticancer effects in various types of cancer. The purpose of the present study was to explore the effect of the new ICT derivative, IC2, targeting SCD1 on breast cancer cells and to explore the specific mechanism.

Methods

Immunohistochemistry and semiquantitative evaluation were performed to detect the expression level of SCD1 in normal and tumor samples. Computer-aided drug design (CADD) technology was used to target SCD1 by molecular docking simulation, and several new ICT derivatives were prepared by conventional chemical synthesis. Cell viability was evaluated by an MTT assay and dead cell staining. SCD1 expression in cancer cells was determined by Western blot and qRT-PCR analyses. The enzymatic activity of SCD1 was evaluated by detecting the conversion rate of [d31] palmitic acid (PA) using Gas chromatography-mass spectrometry (GC–MS). DAPI staining, flow cytometry and Western blot were used to detect cell apoptosis. Mitochondrial membrane potential and reactive oxygen species (ROS) assays were used to determine cell mitochondrial function. Lentiviral transduction was utilized to generate SCD1-overexpressing cell lines.

Results

We found that SCD1 was overexpressed and correlated with poor prognosis in breast cancer patients. Among a series of ICT derivatives, in vitro data showed that IC2 potentially inhibited the viability of breast cancer cells, and the mechanistic study revealed that IC2 treatment resulted in ROS activation and cellular apoptosis. We demonstrated that IC2 inhibited SCD1 activity and expression in breast cancer cells in a dose-dependent manner. Moreover, SCD1 overexpression alleviated IC2-induced cytotoxicity and apoptosis in breast cancer cells.

Conclusions

The new ICT derivative, IC2, was developed to induce breast cancer cell apoptosis by inhibiting SCD1, which provides a basis for the development of IC2 as a potential clinical compound for breast cancer treatment.
Anopheles stephensi in Africa requires a more integrated response
Authors: Mnzava Abraham , Monroe April C. , Okumu Fredros
Published: May 2022

There are increasing reports of the Asian malaria mosquito, Anopheles stephensi invading and spreading in Eastern Africa. We discuss the importance of these invasions in the context of broader challenges facing malaria control in Africa and argue against addressing it as an isolated problem. Anopheles stephensi is only one of multiple biological threats facing malaria control in the region—and is itself an indication of wide-ranging weaknesses in vector surveillance and control programs. Expanded investigations are needed in both urban and rural areas, especially in countries serviced by the Indian Ocean trade routes, to establish the full extent and future trajectories of the problem. More importantly, instead of tackling this vector species as a stand-alone threat, affected countries should adopt more integrated and multi-sectorial initiatives that can sustainably drive and keep out malaria.
Resection of giant malignant solitary fibrous pleural tumor after interventional embolization: a case report and literature review
Authors: Yao Kelin , Zhu Lvcong , Wang Liang , Xia Ruiming , Yang Jianfeng , Hu Wenbin , Yu Zhongqiang
Published: May 2022

Background

Solitary fibrous tumor of the pleura (SFTP) is a rare mesenchymal tumor that arises at various sites and typically originates from the pleura. Most patients with SFTPs are asymptomatic, unless the tumor is large. Approximately 20% of SFTP cases are malignant. There are few reports on imaging diagnoses and interventional treatments of SFTP. Here, we report a case of a giant SFTP that exhibited malignant behavior and underwent successful resection after embolization of the main supply artery of the tumor.

Case presentation

We report a clinical case of a giant SFTP in a 66-year-old Chinese female patient complaining of chest tightness and cough for more than 2 months. Ten years ago, the patient had undergone a chest CT scan at a local hospital for cough. Computed tomography (CT) had revealed a mass in the right thoracic region, which was misdiagnosed as a pulmonary abscess by CT-guided biopsy. Therefore, the patient did not receive appropriate/complete treatment at that time. She was hospitalized again, because CT showed significant enlargement of the right thoracic mass, which caused her obvious symptoms of discomfort. The pathological results of CT-guided biopsy at our hospital confirmed SFTP. Considering the large size of the tumor and the rich blood supply, some of the main blood vessels were treated with embolization before surgical resection. A large tumor, about 23 cm × 16 cm × 15 cm in size, was then successfully removed by thoracic surgery. The diagnosis of malignant SFTP was confirmed by surgical pathology and immunohistochemistry.

Conclusion

Imaging findings of SFTPs are not characteristic, especially when a tumor is large, the diagnosis is difficult, and the final diagnosis still depends on histological and immunohistochemical examinations. The two-stage surgical treatment described here, which involves first embolization of the main supplying artery of the large tumor and then complete surgical resection, is effective and safe for SFTPs. Whether needle biopsy or vascular embolization is performed, intervention plays a crucial role in the diagnosis and treatment of patients with SFTPs.
Surgical outcomes associated with partial upper sternotomy in obese aortic disease patients
Authors: Luo Zeng-Rong , Chen Yi-Xing , Chen Liang-wan
Published: May 2022

Background

Excellent partial upper sternotomy outcomes have been reported for patients undergoing aortic surgery, but whether this approach is particularly beneficial to obese patients remains to be established. This study was developed to explore the outcomes of aortic surgical procedures conducted via a partial upper sternotomy or a full median sternotomy approach in obese patients.

Methods

We retrospectively examined consecutive acute type A aortic dissection patients who underwent aortic surgery in our hospital between January 2015 to January 2021. Patients were divided into two groups based on body mass index: ‘non-obese’ and ‘obese’. We then further stratified patients in the obese and non-obese groups into partial upper sternotomy and full median sternotomy groups, with outcomes between these two sternotomy groups then being compared within and between these two body mass index groups.

Results

In total, records for 493 patients that had undergone aortic surgery were retrospectively reviewed, leading to the identification of 158 consecutive obese patients and 335 non-obese patients. Overall, 88 and 70 obese patients underwent full median sternotomy and partial upper sternotomy, respectively, while 180 and 155 non-obese patients underwent these respective procedures. There were no differences between the full median sternotomy and partial upper sternotomy groups within either BMI cohort with respect to preoperative baseline indicators and postoperative complications. Among non-obese individuals, the partial upper sternotomy approach was associated with reduced ventilation time (P = 0.003), shorter intensive care unit stay (P = 0.017), shorter duration of hospitalization (P = 0.001), and decreased transfusion requirements (Packed red blood cells: P < 0.001; Fresh frozen plasma: P < 0.001). Comparable findings were also evident among obese patients.

Conclusions

Obese aortic disease patients exhibited beneficial outcomes similar to those achieved for non-obese patients via a partial upper sternotomy approach which was associated with significant reductions in the duration of intensive care unit residency, duration of hospitalization, ventilator use, and transfusion requirements. This surgical approach should thus be offered to aortic disease patients irrespective of their body mass index.
Histidine–tryptophan–ketoglutarate solution versus multidose cardioplegia for myocardial protection in cardiac surgeries: a systematic review and meta-analysis
Authors: Albadrani Muayad
Published: May 2022

Background

Surgical procedures in the heart requires protection of the heart from ischemia–reperfusion injury. Cardioplegia is the primary myocardial protective method in use. Histidine–tryptophan–ketoglutarate (HTK) solution is an intracellular cardioplegic solution that was initially used to preserve organs for transplantation.

Methods

A systematic electronic search was conducted in July 2021, in four databases; PubMed, Scopus, Web of Science, and Cochrane Library for eligible randomized controlled trials. The results were screened and the eligible trials were identified. Thereafter, the relevant data were extracted and pooled as mean difference or risk ratio, and 95% confidence interval in an inverse variance method using RevMan software.

Results

This review included 12 trials (n = 1327). HTK solution has resulted significantly in shorter intensive care unit stay (MD = − 0.09; 95% CI [− 0.15, − 0.03], p = 0.006), and shorter hospital stay (MD = − 0.51; 95% CI [− 0.71, − 0.31], p < 0.00001). Moreover, the patients who received the HTK solution had significantly lower levels of creatine kinase (after 4–7 h (MD = − 157.52; 95% CI [− 272.31, − 42.19], p = 0.007), and 24 h (MD = − 136.62; 95% CI [− 267.20, − 6.05], p = 0.04)), as well as creatine kinase muscle brain band (after 44–48 h (MD = − 3.35; 95% CI [− 5.69, − 1.02], p = 0.005)).

Conclusion

HTK solution had the same efficacy and safety as other cardioplegic solutions in most of the clinical parameters. Furthermore, the solution showed superiority in fastening the recovery and protecting the myocardium at the biochemical level. HTK solution provides longer myocardial protection; therefore, it limits surgical interruption. HTK solution can be used as an alternative to the currently used cardioplegic solutions.
High-risk twin pregnancy: case report of an adolescent patient with cystic fibrosis and systemic lupus erythematosus
Authors: Sanseverino Paula Baptista , Hoffmann Anneliese , Machado Sandra , Farias Mariana , Michels Marcus Silva , Sanseverino Maria Teresa Vieira , Marostica Paulo José Cauduro
Published: May 2022

Background

We present the first case to our knowledge of a spontaneous twin pregnancy in a 16-year-old Caucasian patient with cystic fibrosis and systemic lupus erythematosus. Cystic fibrosis is one of the most common autosomal recessive genetic disorders and primarily affects the respiratory and digestive systems. Systemic lupus erythematosus is a chronic inflammatory disease of unknown cause that affects nearly every organ. Patients with cystic fibrosis or systemic lupus erythematosus are progressively having longer life expectancy and better quality of life, which has led a greater number of female patients reporting the desire to become mothers.

Case presentation

We present a case of a Caucasian 16-year-old pregnant with twins being treated for both cystic fibrosis and systemic lupus erythematosus. She has two CFTR mutations: p.F508del and 1812_1G>A. In the second trimester, she was admitted for possible preterm labor, which was successfully stopped. The patient’s nutritional status worsened, and she had a pulmonary exacerbation as well as a flare of systemic lupus erythematosus. At the 28th gestational week, she presented with a massive hemoptysis episode. The cesarean delivery had no complications, and there were no serious immediate postpartum complications.

Discussion and conclusions

While adolescent pregnancies in and of themselves are considered high risk for both the young mothers and their children, they are further complicated when the mother has two chronic diseases and a twin pregnancy. We achieved positive results using a multidisciplinary approach; however, the risks involved were so high that major efforts are to be taken by our medical community to prevent unplanned pregnancies in all patients with cystic fibrosis, especially when a serious comorbidity like the one in this case is present.
Mxc, a Drosophila homolog of mental retardation-associated gene NPAT, maintains neural stem cell fate
Authors: Sang Rong , Wu Cheng , Xie Shanshan , Xu Xiao , Lou Yuhan , Ge Wanzhong , Xi Yongmei , Yang Xiaohang
Published: May 2022

Background

Mental retardation is a complex neurodevelopmental disorder. NPAT, a component of the histone locus body (HLB), has been implicated as a candidate gene for mental retardation, with a mechanism yet to be elucidated.

Results

We identified that mxc, the Drosophila ortholog of NPAT, is required for the development of nervous system. Knockdown of mxc resulted in a massive loss of neurons and locomotion dysfunction in adult flies. In the mxc mutant or RNAi knockdown larval brains, the neuroblast (NB, also known as neural stem cell) cell fate is prematurely terminated and its proliferation potential is impeded concurrent with the blocking of the differentiation process of ganglion mother cells (GMCs). A reduction of transcription levels of histone genes was shown in mxc knockdown larval brains, accompanied by DNA double-strand breaks (DSBs). The subsidence of histone transcription levels leads to prematurely termination of NB cell fate and blockage of the GMC differentiation process. Our data also show that the increase in autophagy induced by mxc knockdown in NBs could be a defense mechanism in response to abnormal HLB assembly and premature termination of NB cell fate.

Conclusions

Our study demonstrate that Mxc plays a critical role in maintaining neural stem cell fate and GMC differentiation in the Drosophila larval brain. This discovery may shed light on the understanding of the pathogenesis of NPAT-related mental retardation in humans.
Claudin18.2 is a novel molecular biomarker for tumor-targeted immunotherapy
Authors: Cao Weijie , Xing Haizhou , Li Yingmei , Tian Wenliang , Song Yongping , Jiang Zhongxing , Yu Jifeng
Published: May 2022

The claudin18.2 (CLDN18.2) protein, an isoform of claudin18, a member of the tight junction protein family, is a highly selective biomarker with limited expression in normal tissues and often abnormal expression during the occurrence and development of various primary malignant tumors, such as gastric cancer/gastroesophageal junction (GC/GEJ) cancer, breast cancer, colon cancer, liver cancer, head and neck cancer, bronchial cancer and non-small-cell lung cancer. CLDN18.2 participates in the proliferation, differentiation and migration of tumor cells. Recent studies have identified CLDN18.2 expression as a potential specific marker for the diagnosis and treatment of these tumors. With its specific expression pattern, CLDN18.2 has become a unique molecule for targeted therapy in different cancers, especially in GC; for example, agents such as zolbetuximab (claudiximab, IMAB362), a monoclonal antibody (mAb) against CLDN18.2, have been developed. In this review, we outline recent advances in the development of immunotherapy strategies targeting CLDN18.2, including monoclonal antibodies (mAbs), bispecific antibodies (BsAbs), chimeric antigen receptor T (CAR-T) cells redirected to target CLDN18.2, and antibody–drug conjugates (ADCs).
The polyG diseases: a new disease entity
Authors: Liufu Tongling , Zheng Yilei , Yu Jiaxi , Yuan Yun , Wang Zhaoxia , Deng Jianwen , Hong Daojun
Published: May 2022

Recently, inspired by the similar clinical and pathological features shared with fragile X-associated tremor/ataxia syndrome (FXTAS), abnormal expansion of CGG repeats in the 5’ untranslated region has been found in neuronal intranuclear inclusion disease (NIID), oculopharyngeal myopathy with leukoencephalopathy (OPML), and oculopharyngodistal myopathy (OPDMs). Although the upstream open reading frame has not been elucidated in OPML and OPDMs, polyglycine (polyG) translated by expanded CGG repeats is reported to be as a primary pathogenesis in FXTAS and NIID. Collectively, these findings indicate a new disease entity, the polyG diseases. In this review, we state the common clinical manifestations, pathological features, mechanisms, and potential therapies in these diseases, and provide preliminary opinions about future research in polyG diseases.
The dural angioleiomyoma harbors frequent GJA4 mutation and a distinct DNA methylation profile
Authors: Tauziède-Espariat Arnault , Pierre Thibaut , Wassef Michel , Castel David , Riant Florence , Grill Jacques , Roux Alexandre , Pallud Johan , Dezamis Edouard , Bresson Damien , Benichi Sandro , Blauwblomme Thomas , Benzohra Djallel , Gauchotte Guillaume , Pouget Celso , Colnat-Coulbois Sophie , Mokhtari Karima , Balleyguier Corinne , Larousserie Frédérique , Dangouloff-Ros Volodia , Boddaert Nathalie , Debily Marie-Anne , Hasty Lauren , Polivka Marc , Adle-Biassette Homa , Métais Alice , Lechapt Emmanuèle , Chrétien Fabrice , Sahm Felix , Sievers Philipp , Varlet Pascale ,   the RENOCLIP-LOC
Published: May 2022

The International Society for the Study of Vascular Anomalies (ISSVA) has defined four vascular lesions in the central nervous system (CNS): arteriovenous malformations, cavernous angiomas (also known as cerebral cavernous malformations), venous malformations, and telangiectasias. From a retrospective central radiological and histopathological review of 202 CNS vascular lesions, we identified three cases of unclassified vascular lesions. Interestingly, they shared the same radiological and histopathological features evoking the cavernous subtype of angioleiomyomas described in the soft tissue. We grouped them together with four additional similar cases from our clinicopathological network and performed combined molecular analyses. In addition, cases were compared with a cohort of 5 soft tissue angioleiomyomas. Three out 6 CNS lesions presented the same p.Gly41CysGJA4mutation recently reported in hepatic hemangiomas and cutaneous venous malformations and found in 4/5 soft tissue angioleiomyomas of our cohort with available data. Most DNA methylation profiles were not classifiable using the CNS brain tumor (version 12.5), and sarcoma (version 12.2) classifiers. However, using unsupervised t-SNE analysis and hierarchical clustering analysis, 5 of the 6 lesions grouped together and formed a distinct epigenetic group, separated from the clusters of soft tissue angioleiomyomas, other vascular tumors, inflammatory myofibroblastic tumors and meningiomas. Our extensive literature review identified several cases similar to these lesions, with a wide variety of denominations. Based on radiological and histomolecular findings, we suggest the new terminology of “dural angioleiomyomas” (DALM) to designate these lesions characterized by a distinct DNA methylation pattern and frequentGJA4mutations.
Social media analysis of car parking behavior using similarity based clustering
Authors: Arhab Nabil , Oussalah Mourad , Jahan Md Saroar
Published: May 2022

This paper investigates car parking users’ behaviors from social media perspective using social network based analysis of online communities revealed by mining the associated hashtags in Twitter. We propose a newinterpretablecommunity detection approach for mapping user’s car parking behavior by combining Clique, K-core and Girvan–Newman community detection algorithms together with a content-based analysis that exploits polarity, relative frequency and dominant topics. Twitter API was used to collect relevant data by tracking popular car-parking hashtags. A social network graph is constructed using a similarity-based analysis. Finally, interpretable communities are inferred by monitoring the outcomes of clique, K-core and Girvan–Newman community detection algorithms. This interpretability is linked to the aggregation of keywords, hashtags and/or location attributes of the tweet messages as well as a visualization module that enables interaction with users. In parallel, a global trend analysis investigates parking types and Twitter influence with respect to both sentiment polarity and dominant trends (extracted using KeyBERT based approach) is performed. The implementation of this social media analytics has uncovered several aspects associated to car-parking behaviors. A comparison with some state-of-the-art community detection methods has also been carried out and revealed some similarities with our developed approach.
Earthquake probability in the National Seismic Hazard Maps for Japan and people’s risk perceptions: the search for more effective expression
Authors: Saito Sayaka , Yasumoto Shinya , Sekiya Naoya
Published: May 2022

This study summarizes the findings of an attitude survey focused on people’s perceptions of seismic hazard maps, which illustrate the risk of an earthquake in each location throughout Japan. These seismic hazard maps depict the likelihood of an earthquake with a seismic intensity of “6 Lower” or more within 30 years, with 3.0% being considered a high possibility. The 3.0% occurrence probability within 30 years can be reworded to 2.0% occurrence probability within 20 years, 0.1% occurrence probability within a year, 4.9% occurrence probability within 50 years, or 39.4% occurrence probability within 500 years when being converted according to a Poisson distribution. In this study, we convert the occurrence probability within 30 years in the seismic hazard maps to obtain that within a year up to that within 1000 years, present the involved risk and demonstrate the yearly probability of the people who see the maps becoming most convinced of the need for countermeasures. Although those are shorter than the occurrence probability within 30 years anyway, it is a characteristic that the peaks existed at the occurrence probability within 10–20 years, not at the occurrence probability within one year or five years. However, the results changed according to the age of the people viewing the maps, and it was when the risk was presented as the occurrence probabilities within 20 and 30 years for the people in their 20–40 s, while it was when the risk was presented the occurrence probabilities within 10 years for the people in their 50–60 s, the perception toward the need of countermeasures increased the most. In addition, regardless of gender and place of residence (coast of the Sea of Japan or the Pacific coast), the perception toward the need of countermeasures peaked at the occurrence probabilities within 10 and 20 years.

Graphical Abstract
Improved succinic acid production through the reconstruction of methanol dissimilation in Escherichia coli
Authors: Guo Feng , Wu Min , Zhang Shangjie , Feng Yifan , Jiang Yujia , Jiang Wankui , Xin Fengxue , Zhang Wenming , Jiang Min
Published: May 2022

Synthetic biology has boosted the rapid development on using non-methylotrophy as chassis for value added chemicals production from one-carbon feedstocks, such as methanol and formic acid. The one-carbon dissimilation pathway can provide more NADH than monosaccharides including glucose, which is conducive for reductive chemicals production, such as succinic acid. In this study, the one-carbon dissimilation pathway was introduced in E. coli Suc260 to enhance the succinic acid production capability. Through the rational construction of methanol dissimilation pathway, the succinic acid yield was increased from 0.91 to 0.95 g/g with methanol and sodium formate as auxiliary substrates in anaerobic fed-batch fermentation. Furthermore, the metabolic flux of by-product pyruvate was redirected to succinic acid together with the CO2 fixation. Finally, through the immobilization on a specially designed glycosylated membrane, E. coli cells are more resistant to adverse environments, and the final yield of succinic acid was improved to 0.98 g/g. This study proved the feasibility of endowing producers with methanol dissimilation pathway to enhance the production of reductive metabolites.

Graphical Abstract
Review of Fire ecology and management: past, present, and future of US forested ecosystems by Cathryn H. Greenberg and Beverly Collins (editors) and 75 contributing authors
Authors: Chaideftou Evgenia
Published: May 2022

Background

Fire is a multifaceted force. Fire activity and risk of fire incidence across US forested ecosystems have accelerated over the last two decades. At the same time, human land-use choices and climate change interacted with fire, in an era we are called to meet specific sustainability goals of reducing CO2 emissions and protecting biodiversity, ecosystems, and their resources. To understand the development of the traits and functions of organisms, and the processes and drivers of natural and human-induced events that cause fire, it is essential to study the fire ecology in the context of human land-use history in the forested ecoregions of the USA.

Results

This book is a research synthesis of the fire ecology in the US forest ecosystems at the regional level. Two main pillars are analyzed across all ecoregions and forest types covered in the book, the drivers and linkages that form fire history and interactions initiated by recurring fire events, and the interdependence of fire with the forest ecosystem dynamics and humans.

Conclusions

The research, policy, and management tools available for managing fire as a disturbance and as prevention and mitigation treatment need cross-boundary revision. It is fundamental to realize the precise role of fire at present and to redefine the role of fire in the short and long term. This book is an invitation to change our perspective and methods towards these directions.
Application of 3-nitrooxypropanol and canola oil to mitigate enteric methane emissions of beef cattle results in distinctly different effects on the rumen microbial community
Authors: Gruninger Robert J. , Zhang Xiu Min , Smith Megan L. , Kung Limin , Vyas Diwakar , McGinn Sean M. , Kindermann Maik , Wang Min , Tan Zhi Liang , Beauchemin Karen A.
Published: May 2022

Background

The major greenhouse gas from ruminants is enteric methane (CH4) which in 2010, was estimated at 2.1 Gt of CO2 equivalent, accounting for 4.3% of global anthropogenic greenhouse gas emissions. There are extensive efforts being made around the world to develop CH4 mitigating inhibitors that specifically target rumen methanogens with the ultimate goal of reducing the environmental footprint of ruminant livestock production. This study examined the individual and combined effects of supplementing a high-forage diet (90% barley silage) fed to beef cattle with the investigational CH4 inhibitor 3-nitrooxypropanol (3-NOP) and canola oil (OIL) on the rumen microbial community in relation to enteric CH4 emissions and ruminal fermentation.

Results

3-NOP and OIL individually reduced enteric CH4 yield (g/kg dry matter intake) by 28.2% and 24.0%, respectively, and the effects were additive when used in combination (51.3% reduction). 3-NOP increased H2 emissions 37-fold, while co-administering 3-NOP and OIL increased H2 in the rumen 20-fold relative to the control diet. The inclusion of 3-NOP or OIL significantly reduced the diversity of the rumen microbiome. 3-NOP resulted in targeted changes in the microbiome decreasing the relative abundance of Methanobrevibacter and increasing the relative abundance of Bacteroidetes. The inclusion of OIL resulted in substantial changes to the microbial community that were associated with changes in ruminal volatile fatty acid concentration and gas production. OIL significantly reduced the abundance of protozoa and fiber-degrading microbes in the rumen but it did not selectively alter the abundance of rumen methanogens.

Conclusions

Our data provide a mechanistic understanding of CH4 inhibition by 3-NOP and OIL when offered alone and in combination to cattle fed a high forage diet. 3-NOP specifically targeted rumen methanogens and partly inhibited the hydrogenotrophic methanogenesis pathway, which increased H2 emissions and propionate molar proportion in rumen fluid. In contrast, OIL caused substantial changes in the rumen microbial community by indiscriminately altering the abundance of a range of rumen microbes, reducing the abundance of fibrolytic bacteria and protozoa, resulting in altered rumen fermentation. Importantly, our data suggest that co-administering CH4 inhibitors with distinct mechanisms of action can both enhance CH4 inhibition and provide alternative sinks to prevent excessive accumulation of ruminal H2.