bluuebunny/crossref_metadata_2025 huggingface dataset:
Longitudinal hair cortisol in bipolar disorder and a mechanism based on HPA dynamics
Authors: Alon Uri , Milo Tomer , Maimon Lior , Cohen Ben , Haran Dafna , Segman Dror , Danon Tamar , Bren Anat , Mayo Avi , Rappaport Gadi Cohen , McInnis Melvin
Published: July 2023

Bipolar disorder (BD) is a dynamic disease in which mania, depression and mixed states vary on a timescale of months to years. BD patients characteristically exhibit elevated levels of the hormone cortisol. Persistently elevated cortisol can also cause mood episodes in a substantial fraction of the general population. Although BD is a dynamic disease that is related to cortisol, longitudinal cortisol dynamics in BD have rarely been studied. Here we use hair to measure past cortisol where each cm of hair corresponds to a month of growth. Cortisol was measured in 12 cm hair samples from people with BD (n = 26) and controls (n = 59), corresponding to one year of cortisol data. We found that hair cortisol exhibited a frequency spectrum with enhanced year-scale fluctuations whose amplitude was about 4-fold higher on average in BD compared to controls. Cortisol in the proximal 2 cm hair segment correlated with mood scales that report on mood in the past two months. In line with the notion that cortisol correlates with mood, we find that the mean frequency spectrum of depression (n = 266) and mania (n = 273) scores from a large longitudinal study of BD is similar to the hair cortisol spectrum from the present cohort. Taken together, these results suggest a mechanism for BD as the intersection of two neuropsychological traits: cortisol-induced mood episodes (CIM) and high emotional reactivity (ER). High ER causes fluctuations in which cortisol is elevated for months, as shown by a mathematical model of the hypothalamic-pituitary-adrenal (HPA) axis that regulates cortisol. In individuals with CIM, the magnitude of these persistent cortisol fluctuations can be high enough to trigger mood episodes. Thus, this study combines longitudinal cortisol measurements and mathematical modeling to provide a potential mechanistic link between the timescales of cortisol and moods in BD.
Heterologous expression and fibrillary characterization of the microtubule-binding domain of tau associated with tauopathies
Authors: Peng Chong , Wei Wei , Zhang Huitu , Wang Ying , Chang Baogen , Zhao Wenping , Jia Longgang , Li Li , Lu Fuping , Liu Fufeng
Published: July 2023

Background
Neurofibrillary tangles (NFTs) are one of the most common pathological characteristics of Alzheimer's disease (AD). The NFTs are mainly composed of hyperphosphorylated microtubule-associated tau. Thus, recombinant tau is urgently required for the study of its fibrillogenesis and its associated cytotoxicity.
Methods and Results
Heterologous expression, purification, and fibrillation of the microtubule-binding domain (MBD) of tau (tau&MBD) were performed. The tau&MBD was heterologously expressed in E. coli. Ni-chelating affinity (Ni-NTA) chromatography was then performed to purify the target protein. Thereafter, tau&MBD was systematically identified using the SDS-PAGE, western blot and MALDI-TOF MS methods. The aggregation propensity of the tau&MBD was explored by both the thioflavin T fluorescence and atomic force microscopy experiments.
Conclusions
The final yield of the recombinant tau&MBD was ~20 mg·L-1. It is shown that Tau&MBD self-assembled into the typical fibrils. Finally, the in vitro cytotoxicity of tau&MBD aggregates was validated using PC12 cells. The heterologously expressed tau in this study can be further used in the investigation of the biophysical and cellular cytotoxic properties of tau.
Ultrafast Silicon Optical Nonlinear Activator for Neuromorphic Computing
Authors: Yan Siqi , Zhou Ziwen , Liu Chen , Zhao Weiwei , Liu Jingze , Jiang Ting , Peng Wenyi , Xiong Jiawang , Wu Hao , Zhang Chi , Ding Yunhong , Ros Francesco Da , Xu Xingyuan , Xu Kun , Ming Tang
Published: July 2023

Optical neural networks (ONNs) have shown great promise in overcoming the speed and efficiency bottlenecks of artificial neural networks (ANNs). However, the absence of high-speed, energy-efficient nonlinear activators significantly impedes the advancement of ONNs and their extension to ultrafast application scenarios like autonomous vehicles and real-time intelligent signal processing. In this work, we designed and fabricated a novel silicon-based ultrafast all-optical nonlinear activator, leveraging the hybrid integration of silicon slot waveguides, plasmonic slot waveguides, and monolayer graphene. We utilized double-balanced detection and synchronous pump-probe measurement techniques to experimentally evaluate the static and dynamic characteristics of the activators, respectively. Exploiting the exceptional picosecond scale photogenerated carrier relaxation time of graphene, the response time of the activator is markedly reduced to ~93.6 ps. This response time is approximately five times faster than electronic neural networks, establishing our all-optical activator as the fastest known in silicon photonics to our knowledge. Moreover, the all-optical nonlinear activator holds a low threshold power of 5.49 mW and a corresponding power consumption per activation of 0.51 pJ. Furthermore, we confirm its feasibility and capability for use in ONNs by simulation, achieving a high accuracy of 96.8% for MNIST handwritten digit recognition and a mean absolute error of less than 0.1 dB for optical signal-to-noise ratio monitoring of high-speed optical signals. This breakthrough in speed and energy efficiency of all-optical nonlinear activators opens the door to significant improvements in the performance and applicability of ONNs, ushering in a new era of advanced artificial intelligence technologies with enormous potential.
Phenotypic and transcriptional analysis of the antimicrobial effect of lactic acid bacteria on carbapenem-resistant Acinetobacter baumannii: Lacticaseibacillus rhamnosus CRL 2244 an alternative strategy to overcome resistance?”
Authors: Rodriguez Cecilia , Ramlaoui Dema , Georgeos Nardin , Gasca Briea , Leal Camila , Subils Tomás , Tuttobene Marisel R , Sieira Rodrigo , Salzameda Nicholas T. , Bonomo Robert A. , Raya Raúl , Ramirez María Soledad
Published: July 2023

Carbapenem-resistant Acinetobacter baumannii (CRAB) is a recognized nosocomial pathogen with limited antibiotic treatment options. Lactic acid bacteria (LAB) constitute a promising therapeutic alternative. Here we studied the antibacterial properties of a collection of LAB strains using phenotypic and transcriptomic analysis against A. baumannii clinical strains. One strain, Lacticaseibacillus rhamnosus CRL 2244, demonstrated a potent inhibitory capacity on A. baumannii with a significant killing activity. Scanning electron microscopy images showed changes in the morphology of A. baumannii with an increased formation of outer membrane vesicles. Significant changes in the expression levels of a wide variety of genes were also observed. Interestingly, most of the modified genes were involved in a metabolic pathway known to be associated with the survival of A. baumannii. The paa operon, Hut system, and fatty acid degradation were some of the pathways that were induced. The analysis reveals the impact of Lcb. rhamnosus CRL 2244 on A. baumannii response, resulting in bacterial stress and subsequent cell death. These findings highlight the antibacterial properties of Lcb. rhamnosus CRL 2244 and its potential as an alternative or complementary strategy for treating infections. Further exploration and development of LAB as a treatment option could provide valuable alternatives for combating CRAB infections.
Linking rhizospheric microbiota and metabolite interactions with harvested aboveground carbon and soil carbon of lakeshore reed wetlands in a subtropical region
Authors: Wang Junli , Fu Zishi , Qiao Hongxia , Liu Fuxing , Bi Yucui
Published: July 2023

Aims
Lakeshore wetlands are global carbon (C) hotspots, but their role in C sequestration has been largely overlooked. The rhizosphere has a complex interaction of microbiota and metabolites, which plays an important role in wetland C cycling. This study aims to understand how the rhizospheric interactions affects harvested aboveground C and soil C of lakeshore wetlands in a subtropical region.
Methods
An investigation of five lakeshore reed (Phragmites australis) wetlands at the similar latitudes of the Lower Yangtse Valley in China was carried out to explore the relationship of rhizospheric interactions with harvested aboveground C and soil C. The plant traits and soil physicochemical properties were determined due to their important role in affecting rhizosphere interactions.
Results
Plant traits and soil physicochemical properties significantly differed among the sites, while aboveground C fixation did not significantly differ. The soil organic C (SOC) content of the topsoil was accounting for the majority of the soil total C at most sites, except for the wetland at the Yangtze River estuary with higher soil pH and conductivity, whose soil inorganic C (SIC) accounted for almost half. Bacterial community and metabolite composition were significantly partitioned across the region. Structural equation modeling revealed the rhizospheric interactions positively affected aboveground C and SOC, but negatively affected SIC. Their effects on soil C content were stronger than those on aboveground C fixation.
Conclusions
The rhizosphere exhibited the direct and indirect effects on harvested aboveground C and soil C by altering microbial community structure and metabolite composition.
Preoperative Systemic Inflammatory Measures as a Maker of Recurrence in Patients with Early Esophageal Squamous Cell Cancer:a Cross-Sectional Retrospective Study
Authors: Jiao Chenyang , Qian Yun , Yang Bin , Fu Yiwei
Published: July 2023

Background. It is reported that systemic inflammatory measures including neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and systemic immune-inflammatory index (SII) were associated with prognosis in patients with advanced esophageal squamous cell carcinoma (ESCC),but the correlation between the prognosis of early ESCC and above parameters is still unclear.
Objective. The aim of this study was to evaluate whether preoperative systemic inflammatory measures were associated with ectopic recurrence in patients with early ESCC after endoscopic submucosal dissection (ESD).
Methods. Preoperative systemic inflammatory measures were obtained in 120 patients who received the treatment of ESD for early ESCC from July 2015 to September 2018. Clinicopathological characteristics were compared between recurrence and non-recurrence groups. Receiver operating characteristic (ROC) curves were plotted, and the areas under the ROC curves (AUROCs) were compared to verify the accuracy of each measure in predicting recurrence.
Results. NLR and SII were significantly higher in patients suffering ectopic recurrence than non-recurrence patients (NLR, 2.38 ± 1.30 vs 3.32 ± 1.00, p = 0.016; SII, 349.79 ± 223.21 vs 511.49 ± 208.63, p = 0.029). NLR > 2.24 (90% sensitivity and 57.3%, specifically, p = 0.005, AUROC = 0.771) and SII > 345.49 (90% sensitivity and 58.2% specifically, p = 0.007, AUROC = 0.757) were predictors of recurrence .
Conclusion. Patients with higher NLR and SII levels were at higher risk of ectopic recurrence. NLR and SII should be clinically useful biomarkers to guide early ESCC postoperative follow-up procedures.
Accurate nowcasting of cloud cover at solar photovoltaic plants
Authors: Min Min , Xia Pan , Zhang Lu , Li Jun , Wang Yun , Yu Yu , Jia Shengjie
Published: July 2023

Accurate nowcasting of cloud cover or fraction (CF) and its movement is still an intractable challenge for stable solar photovoltaic (PV) electricity generation. By combining continuous radiance images measured by geostationary satellite and an advanced recurrent neural network, we develop a novel nowcasting algorithm for predicting CF at the leading time of 0–4 hours at PV plants. Based on this algorithm, a cyclically updated prediction system is also established and tested at two PV plants and several stations with CF observations in China. The results demonstrate that the CF nowcasting is efficient, high quality and adaptable. Particularly, it shows an excellent forecast performance within the first 2-hour leading time, with an average correlation coefficient close to 0.90(0.80) between predicted CF and actual CF(power generation at PV plants). Our findings highlight the benefits and potential of this technique to improve the competitiveness of solar PV energy in electricity market.
Stacking of Recessive Alleles for P34, Lectin, KTI and 7S α'-subunit Proteins in Soybean [Glycine max (L.) Merr.]
Authors: Ly Sarath , Lee Jeong Hwan , Oh Hyeon Su , Kim Se Yeong , Choi Sang Woo , chung Jongil
Published: July 2023

Soybean [Glycine max (L.) Merr.] is an excellent source of protein for humans, but several allergenic and antinutritional components such as P34, lectin, Kunitz Trypsin Inhibitor (KTI), and 7S α′ subunit proteins exist in the raw mature seed. Genetic elimination of these factors would be the appropriate method to improve soybean food quality. The objective of this research was to develop a new soybean line with a tetra null recessive genotype (titilelecgy1cgy1p34p34) based on Mendelian inheritance. Four germplasms were used to develop the tetra null strain. A total of 322 F2 seeds were obtained from the cross of parent 1 and parent 2. For four proteins of KTI, lectin, 7S α' subunit and P34, and segregation ratio of 107:30:37:8:35:9:14:3:33:9:14:0:13:3:5:2 was observed (χ2 = 9.41, P = 0.9 ~ 0.5). Two F2 seeds possessing the tetra null genotype (cgy1cgy1titilelep34p34) were selected. Confirmation of the Tetra null genotype (titilelecgy1cgy1p34p34 - absence of KTI, lectin, 7S α' subunit and P34 proteins) was verified on the random F3 seeds of the two lines selected. The seed color for both selection lines was yellow and the hilum color was buff. The seed weights (g/100 seeds) for the two selected lines were 12.2 g and 12.0 g. This paper is the first to investigate the genetic segregation ratio and selection of tetra null genotype for four proteins of P34, lectin, KTI and 7S α' subunit proteins in soybean.
The safety profile of tramadol combined with nicotine on liver and testicles: Evidence from endoplasmic reticulum stress
Authors: Ghorab Doaa , Rub Ejlal Abu- , Gharaibeh Mohamed Hamdi , Khasawneh Ramada R , Almazari Rawan A. , Al-Emam Ahmed , HELALY AHMED
Published: July 2023

Background: Tramadol is a substance of abuse in the Middle East, and smoking is extremely common among the population.
Methods:An experimental study was performed on Sprague Dawley rats to explore the effect of both nicotine and tramadol on the liver and testes. Tramadol was administered at 10 mg/kg and 20 mg/kg in the liver and testes, respectively, while nicotine was administered at 125 mcg/kg. Western blot analysis of BIP (immunoglobulin heavy-chain binding protein) and CHOP (CCAAT-enhancer-binding protein homologous protein) revealed that fewer problems were induced by adding nicotine to tramadol.
Conclusions: The results showed mild endoplasmic reticulum stress markers (BIP and CHOP), autophagy, and apoptosis in the liver samples. Histological examination revealed stable spermatogenesis with normal androgen blood levels in the different groups.
Large scale rice germplasm screening for identification of novel brown planthopper resistance sources
Authors: yang ke , Liu Hongmei , Jiang Weihua , Hu Yinxia , Zhou Zhiyang , An Xin , Miao Si , Qin Yushi , Du Bo , Zhu Lili , He Guangcun , Chen Rongzhi
Published: July 2023

Rice (Oryza sativa L.) is a staple food crop globally. Brown planthopper (Nilaparvata lugens Stål, BPH) is the most destructive insect that threatens rice production annually. More than 40 BPH resistance genes have been identified so far, which provide valuable gene resources for marker-assisted breeding against BPH. However, it is still urgent to evaluate rice germplasms and to explore more new wide-spectrum BPH resistance genes to combat newly occurring virulent BPH populations. To this end, 560 germplasm accessions were collected from the International Rice Research Institute (IRRI), and their resistance to current BPH population of China was examined. A total of 105 highly resistant materials were identified. Molecular screening of BPH resistance genes in these rice germplasms was conducted by developing specific functional molecular markers of eight cloned resistance genes. Twenty-three resistant germplasms were found to contain none of the known BPH resistance genes. These accessions also exhibited a variety of resistance mechanisms as indicated by an improved insect weight gain (WG) method, suggesting the existence of new resistance genes. One new BPH resistance gene, Bph44(t), was identified in rice accession IRGC 15344 and preliminarily mapped to a 0–2 Mb region on chromosome 4. This study systematically sorted out the corresponding relationships between BPH resistance genes and germplasm resources using a functional molecular marker system. Newly explored resistant germplasms will provide valualble donors for the identification of new resistance genes and BPH resistance breeding programs.
Germline Genetic NBN Variation and Predisposition to B-cell Acute Lymphoblastic Leukemia in Children
Authors: Yang Jun , Escherich Carolin , Chen Wenan , Li Yizhen , Yang Wenjian , Nishii Rina , Li Zhenhua , Raetz Elizabeth , Devidas Meenakshi , Wu Gang , Nichols Kim , Inaba Hiroto , Pui Ching-Hon , Jeha Sima , Camitta Bruce , Larsen Eric , Hunger Stephen , Loh Mignon
Published: July 2023

Biallelic mutation in the DNA-damage repair gene NBN is the genetic cause of Nijmegen Breakage Syndrome, which is associated with predisposition to lymphoid malignancies. Heterozygous carriers of germline NBN variants may also be at risk for leukemia development, although this is much less characterized. We systematically examined the frequency of germline NBN variants in pediatric B-ALL and identified 25 putatively damaging NBN coding variants in 50 of 4,183 B-ALL patients. Compared with the frequency of NBN variants in 118,479 gnomAD non-cancer controls we found significant overrepresentation in pediatric B-ALL (p=0.004, OR=1.77). Most B-ALL-risk variants were missense and cluster within the NBN N-terminal domains. Using two functional assays, we verified 14 of 25 variants with severe loss-of-function phenotypes and thus classified these as pathogenic or likely pathogenic. Finally, we found that heterozygous germline NBN variant carriers showed similar survival outcomes relative to those with WT status. Taken together, our findings provide novel insights into the genetic predisposition to B-ALL, the impact of NBN variants on protein function and suggest that heterozygous NBN variant carriers may safely receive B-ALL therapy.
Angelica Sinensis Polysaccharide Protects Chondrocytes from Ferroptosis in Osteoarthritis
Authors: Yin Guangrong , Yu Yunyuan , Ni Su , Wang Jiahao , Zhao Chenyu , Xu Chao , Wang Liangliang , Zhou Baojun , Zhao Gongying , Wang Yuji
Published: July 2023

Background
Osteoarthritis (OA) is one of the most common degenerative diseases. Accumulating evidence suggests that ferroptosis may be important in the progression of OA. Angelica Sinensis polysaccharide (ASP), a traditional Chinese medicine, possesses antioxidative, anti-inflammatory and anti-apoptotic properties. However, it is unclear whether ASP can slow down the progression of osteoarthritis by suppressing ferroptosis.
Methods
Collection of postoperative joint cartilage from patients who underwent total knee arthroplasty (TKA), detection of ferroptosis-related markers including glutathione peroxidase 4 (GPX4) and ferritin heavy polypeptide 1 (FTH1) expression, as well as glutathione (GSH) and malondialdehyde (MDA) levels, and investigation of the correlation between ferroptosis and osteoarthritis. Chondrocytes were isolated and cultured, stimulated separately with IL-1β or erastin, rescued by ASP and ferroptosis inhibitors, and changes in cell viability and ferroptosis were detected. A destabilization of the medial meniscus surgical model (DMM) model was established in mice, and ASP was administered orally at different dosages to evaluate its therapeutic effect and level of ferroptosis.
Results
First, the expression of GPX4, FTH1 and reduced GSH was lower while the MDA level was up-regulated in cartilage of the OA group as compared to the control group. Moreover, chondrocyte ferroptosis induced by Erastin or IL-1β was rescued by the application of ASP or ferroptosis inhibitors in vitro. Further, the anti-ferroptotic effect of ASP was related to enhanced nuclear transfer of Nrf2 and decreased activation of NF-κB as indicated by down-regulated expression of p-p65. Next, the in vivo experiments showed that ASP alleviated the cartilage damage of mice joints induced by DMM.
Conclusions
In summary, ASP can attenuate chondrocyte ferroptosis through the Nrf2/NF-κB pathway in the progression of OA, suggesting that ASP may a potential inhibitor of ferroptosis for the treatment of OA.
Association of polymorphism rs74810361 of IP-10 gene with infection of acute respiratory syndrome-corona virus- type 2
Authors: Jandel Farinoosh , Sharifi Zohreh , Nouri Hamid Reza , Khazaili Ali Arab
Published: July 2023

Introduction
CXCL10 is a pro-inflammatory chemokines that have the role in the cytokine storm and the development of severe lung impairment in COVID-19 patients. Considering there have been no studies related to the association of chemokine IP-10 polymorphism with COVID-19, in this study, this association in patients with COVID-19 was compared with healthy blood donors by PCR-RFLP method.
Method:
In this case-control study, 80 COVID-19 infected patients and 80 control donors were selected. After genomic DNA extraction, amplification of the desired gene fragment was done by PCR-method. In order to investigate specified polymorphism, enzymatic digestion with XbaI was done; then the defined bands were detected by electrophoresis. Also, the severity of the disease was analyzed in terms of polymorphism in the subgroup of patients.
Results:
Correlation analysis between the severity of the disease and IP-10 polymorphism, showed that C1596T mutant allele is associated with an increased probability to infect with the severe phase of COVID-19 disease in male patients (p- value=0.02). The presence of T allele in patients with SpO2 <88 compared to patients with SpO2 >88 was also confirmed in these patients significantly (p-value₌ 0.017).
Conclusions:
A significant relationship was observed between the severity of the disease and TT or CT genotype of C1596T polymorphism in the male group. However, there had no significant relationship between the T allele in SNP C1596T and the susceptibility to the covid-19 disease.
Efficacy of Topical Cetirizine 1% in Treatment of Androgenic Alopecia: Clinical and Dermoscopic Evaluation
Authors: Galal Sara , Mahmoud Wafaa , Ahmed Noha
Published: July 2023

Background: Androgenetic alopecia (AGA) is a form of non-scarring scalp hair loss that affects more than 50% of males. Prostaglandins would have an important role in hair growth and their action is variable according to their class. Based on these and the scarce studies that suggest the effectiveness of cetirizine on AGA we carried out this study.
Aim of Work: Dermoscopic evaluation of the efficacy of topical cetirizine 1 % in male patients with androgenetic alopecia.
Materials and methods: A prospective study was carried out on 50 Egyptian male patients treated with topical cetirizine 1% formula once daily. The clinical and dermoscopic examination was done before, during, and after treatment to evaluate the response.
Results: There was a significant increase in the number of terminal hairs, a decrease in the number of vellus hair, and hair diameter diversity after treatment. There was mild to good photographic improvement.
Conclusion: Topical cetirizine 1 % had a promising effect in the treatment of AGA in men after 4 months of treatment. It also is having an excellent safety profile which could be an excellent option in the near future for the patients.
Solutions of some Integro-Differential problems by an anisotropic singular perturbation method
Authors: Benaoudia Djamila
Published: July 2023

The goal of the this note is to study the existence of solutions to some integro-differential problems. Using the singular perturbations method, one can show the existence of such solutions. The perturbed problems are classical, nonlocal, non-linear parabolic problems and the existence of solutions for this problem is insured by the Schauder fixed point theorem. The limit of the solutions of the nonlocal parabolic problem are solutions of the our integro-differential problem.
AMS Subject Classifications: 35K67, 35B40, 35B25, 35K55, 35K15, 45K05.
Surgical Management of Craniospinal Axis Malignant Peripheral Nerve Sheath Tumors: A Single-Institution Experience and Literature Review
Authors: Chowdhury Ajmain , Vivanco-Suarez Juan , Teferi Nahom , Belzer Alex , Al-Kaylani Hend , Challa Meron , Lee Sarah , Buatti John , Hitchon Patrick
Published: July 2023

Background Malignant peripheral nerve sheath tumor (MPNST) is an exceedingly rare and aggressive tumor, with limited literature on its management. Herein, we present our series of surgically managed craniospinal MPNSTs, analyze their outcomes, and review the literature.Methods We retrospectively reviewed surgically managed primary craniospinal MPNSTs treated at our institution between January 2005 and May 2023. Patient demographics, tumor features, and treatment outcomes were assessed. Neurological function was quantified using the Frankel grade and Karnofsky performance scores. Descriptive statistics, rank-sum tests, and Kaplan-Meier survival analyses were performed.Results Eight patients satisfied the inclusion criteria (4 male, 4 female). The median age at presentation was 38 years (range 15–67). Most tumors were localized to the spine (75%), and 3 patients had neurofibromatosis type 1. The most common presenting symptoms were paresthesia (50%) and visual changes (13%). The median tumor size was 3 cm, and most tumors were oval-shaped (50%) with well-defined borders (75%). Six tumors were high grade (75%), and gross total resection was achieved in 5 patients, with subtotal resection in the remaining 3 patients. Postoperative radiotherapy and chemotherapy were performed in 6 (75%) and 4 (50%) cases, respectively. Local recurrence occurred in 5 (63%) cases, and distant metastases occurred in 2 (25%). The median overall survival was 26.7 months. Five (63%) patients died due to recurrence.Conclusions Primary craniospinal MPNSTs are rare and have an aggressive clinical course. Early diagnosis and treatment are essential for managing these tumors. Maximal resection, low-grade pathology, young age (< 30), and adjuvant radiotherapy were associated with improved survival.
TOB1 and TOB2 mark distinct RNA processing granules in differentiating lens fiber cells
Authors: Perez Rafaela C. , Yang Xenia , Familari Mary , Martinez Gemma , Lovicu Frank J. , Hime Gary R , de Iongh Robb
Published: July 2023

Lens fiber cell differentiation involves a complex interplay of growth factor signals and tight control of gene expression via transcriptional and post-transcriptional regulators. Recent studies have demonstrated an important role for RNA-binding proteins, functioning in ribonucleoprotein granules, in regulating post-transcriptional expression during lens development. Here we have documented the expression of TOB1 and TOB2, members of the BTG/TOB family of RNA-binding proteins, in the developing lens and examine the phenotype of mice that lack Tob1. Both Tob1 and Tob2 mRNA were detected by RT-PCR in epithelial and fiber cells of embryonic and postnatal lenses. By in situ hybridization, Tob1 and Tob2 mRNA were most intensely expressed in the early differentiating fibers, with weaker expression in anterior epithelial cells, and were down-regulated in the germinative zone of E15.5 lenses. TOB1 protein was detected from E11.5 to E16.5 and was predominantly detected in large cytoplasmic puncta in early differentiating fiber cells, often co-localizing with the P-body marker, DCP2. Occasional nuclear puncta were also observed. By contrast, TOB2 was detected in a series of interconnected peri-nuclear granules, in later differentiating fiber cells of the inner cortex. TOB2 did not co-localize with DCP2 but partially co-localized with a marker of stress granules (EIF3B). These data suggest that TOB1 and TOB2 are involved with different parts of the mRNA processing cycle in lens fiber cells. In vitro experiments using rat lens epithelial explants treated with or without a fiber differentiating dose of FGF2 showed that both TOB1 and TOB2 were up-regulated during FGF-induced differentiation. In differentiating explants, TOB1 also co-localized with DCP2 in large cytoplasmic granules. Analyses of Tob1-/- mice revealed relatively normal lens morphology but a subtle defect in cell cycle arrest of some cells at the equator and in the lens fiber mass of E13.5 embryos. Overall, these findings suggest that TOB proteins play distinct regulatory roles in RNA processing during lens fiber differentiation.
PRESTO: A Recommender of Musical Collaborations based on Heterogeneous Graph Neural Networks
Authors: Terroso-Saenz Fernando , Soto Jesus , Muñoz Andres , Roose Philippe
Published: July 2023

The music industry is now more complex and competitive than ever before. In recent years, the search for collaborations with other artists has become a common strategy of musicians to maintain their presence in the sector. Besides, existing music streaming services such as Spotify have exposed large data feeds that can be used to develop innovative services within the realm of music. In this context, the present work introduces PRESTO, a novel recommendation system to suggest musicians new collaborations with other artists by means of an ensemble of Graph Neural Networks. The system is fed with an heterogeneous graph representing the time evolution and the stationary aspects of a musician's career. Finally, the proposal has been evaluated with a dataset comprising more than 200,000 artists, with an average F1 score above 0.75.
Efficient Fuzzy-logic Control of Indoor Mobile Robots Evolving in Static and Dynamic Workspaces
Authors: Hentout Abdelfetah , Maoudj Abderraouf , Kouider Ahmed
Published: July 2023

This paper presents an efficient motion control approach for indoor mobile robots in both static and dynamic workspaces. The proposed system consists of two layers. In the Optimization Layer (Global planner), a Deterministic Constructive Algorithm (DCA) generates the best path for the mobile robot, represented as a set of nodes, to move from the initial position (Source) to the final position (Target) while avoiding the static obstacles. The Control layer (Local planner), which is the main focus of this article, consists of an Efficient Fuzzy-Logic Controller (EFLC), mimicking the human reasoning, to avoid the dynamic obstacles and drive safely the mobile robot along the generated path. The strength of the proposed EFLC approach is shown using V-REP software in dynamic workspaces.
Artificial Intelligence assisted prediction of land surface temperature (LST) based on significant air pollutants over the Annamayya district of India
Authors: Mogaraju Jagadish Kumar
Published: July 2023

Remote sensing (RS), Geographic information systems (GIS), and Machine learning (Artificial Intelligence) can be integrated to predict land surface temperatures (LST) based on the data related to carbon monoxide (CO), Formaldehyde (HCHO), Nitrogen dioxide (NO2), Sulfur dioxide (SO2), absorbing aerosol index (AAI), and Aerosol optical depth (AOD). In this study, LST was predicted using machine learning classifiers, i.e., Extra trees classifier (ET), Logistic regressors (LR), and Random Forests (RF). The accuracy of the LR classifier (0.89 or 89%)is higher than ET (82%) and RF (82%) classifiers. Evaluation metrics for each classifier are presented in the form of accuracy, Area under the curve (AUC), Recall, Precision, F1 score, Kappa, and MCC (Matthews correlation coefficient). Based on the relative performance of the ML classifiers, it was concluded that the LR classifier performed better. Geographic information systems and RS tools were used to extract the data across spatial and temporal scales (2019 to 2022). In order to evaluate the model graphically, ROC (Receiver operating characteristic) curve, Confusion matrix, Validation curve, Classification report, Feature importance plot, and t- SNE (t-distributed stochastic neighbor embedding) plot were used. On validation of each ML classifier, it was observed that the RF classifier returned model complexity due to limited data availability and other factors yet to be studied post data availability. Sentinel-5-P and MODIS data are used in this study.
RESPONSIBILITY OF A LAND DEED OFFICIAL IN A MERGER LAWSUITS FOR TORT AND DEFAULT (DECISION STUDY: 217/PDT.G/2017/PN.MLG)
Authors:   Master Student,FacultyofLaw,JenderalSoedirmanUniversity,Indonesia., Adam Satrio Rachmadhanto Dimas , Lisiani Prihatinah Tri ,   AssociateProfessor,FacultyofLaw, JenderalSoedirmanUniversity,Indonesia., a Sulistyandari ,   Senior Lecturer, FacultyofLaw,JenderalSoedirmanUniversity,Indonesia., Wahyu Handayani Sri ,   Senior Lecturer,FacultyofLaw, JenderalSoedirmanUniversity,Indonesia.
Published: July 2023

The judges legal consideration in combining tort and default claims is justified. In District Court Decision Number 217/Pdt.g/2017/PN.Mlg stated that Defendant I had been proven to have committed an act of default for not paying off the dependents on the payment for the sale and purchase of land and buildings, and Defendant II had been proven to have committed an torfOnrecht for making and using notarial sale and purchase agreement in violation of the law, so that it caused losses to the Plaintiff.The research method used is normative juridical, using secondary data from library research. The panel of judges in accepting the merger of tort and default lawsuits was carried out by analyzing whether the two lawsuits were closely related. So if the two lawsuits have a close relationship between one and the other lawsuit, then the merger lawsuit can be justified, where the merger of the lawsuit can create a simple, fast and light cost court, and can avoid conflicting decisions.
IDIOPATHIC PULMONARY HEMOSIDERIN: ABOUT AN UNUSUAL CASE
Authors:   Pneumopediatric and Allergology Unit, Paediatric Department A, Children University Hospital Mohammed VI, FMPM Cadi Ayyad Marrakesh, Morocco., EL Fakiri Karima , Draiss Ghyzlane ,   Pneumopediatric and Allergology Unit, Paediatric Department A, Children University Hospital Mohammed VI, FMPM Cadi Ayyad Marrakesh, Morocco., Rada Noureddine ,   Pneumopediatric and Allergology Unit, Paediatric Department A, Children University Hospital Mohammed VI, FMPM Cadi Ayyad Marrakesh, Morocco., Bouskraoui Mohammed ,   Pneumopediatric and Allergology Unit, Paediatric Department A, Children University Hospital Mohammed VI, FMPM Cadi Ayyad Marrakesh, Morocco.
Published: July 2023

Introduction: idiopathic pulmonary hemosiderosis is a rare disease of unknown etiology. Case report: This is a 12-year-old boy. The beginning of its symptomatology dates back to one year by several episodes of low-abundance hemoptysis associated with cough and night sweats. He had also a microcytic hypochromic anemia (Hb=4.4) transfused 3 times. . Clinical examination found discoloured skin paleness and conjunctival The count showed microcytic hypochromic anemia with HB:5.7 g/dl. A chest x-ray showed a discrete alveolar syndrome. A tuberculous assessment in particular an IDR tuberculin 3 BK expectorations and GÃ¨nexpert were negative. A chest CT scan showed bilateral interstitial syndrome. A bronchoscopy with bronchoalveolar lavage showed the existence of sidephages with a Golde score calculated on 240 Perls staining . A balance of system diseases including antinuclear antibodies were negative. Ac anti transglutaminase IgA were negative. A heart ultrasound did not show a pulmonary hypertension. In fine, the diagnosis was a primary pulmonary hemosidesis. Management consisted of a blood cell transfusion a prenisolone-based corticosteroid 2 mg/kg/d for 3 months. After two months, the improvement was full. Conclusion: We must think about Hemosidesis idiopathic if we have hemoptysis chronic anemia and pulmonary infiltrates and do a Bronchoalveolar lavage to confirm diagnosis.
A clinical case of signet ring cell carcinoma of stomach in a child. Endoscopic diagnostics
Authors: Lozovaya V. V. , Gusarova O. A. , Malikhova O. A. , Besova N. S. , Suleymanova A. M. , Sinyagina Yu. V. , Tumanyan A. O.
Published: July 2023

Malignant neoplasms (MNP) of the gastrointestinal tract in children are extremely rare and account for 1.2 % of all MNP. According to world literature, the incidence of gastric cancer is no more than 0.05 % of all cases of MNP of the gastrointestinal tract in children. In national and foreign literature, only isolated cases of stomach cancer in children and adolescents aged 10 to 18 years are described. Clinical manifestations of the disease, as in patients over the age of 18, are non-specific – epigastric pain, loss of appetite, weight loss, ascites, anemia, melena and others. In this regard, the diagnosis occurs at the late stages of the disease and, therefore, is characterized by an unfavorable prognosis. Gastric signet ring cell carcinoma (SRCC) is an uncommon histologic subtype of adenocarcinoma and usually occurs in people aged 20 to 40 years. We would like to report an extremely rare case of SRCC in a 14-year-old child.
The Natural Protoalkaloid Methyl-2-Amino-3-Methoxybenzoate (MAM)
Alleviates Positive as well as Cognitive Symptoms in Rat and Mouse
Schizophrenia Models
Authors: Bright Yami , Maas Dorien A. , Verheij Michel M.M. , Paladini Maria S. , Amatdjais-Groenen Helene I.V. , Molteni Raffaella , Riva Marco A. , Martens Gerard J.M. , Homberg Judith R.
Published: July 2023

The development of new antipsychotics with pro-cognitive properties and less side effects
represents a priority in schizophrenia drug research. In this study, we present for the first time a preclinical
exploration of the effects of the promising natural atypical antipsychotic Methyl-2-Amino-3-
Methoxybenzoate (MAM), a brain-penetrable protoalkaloid from the seed of the plant Nigella damascena.
Using animal models related to hyperdopaminergic activity, namely the pharmacogenetic apomorphine
(D2/D1 receptor agonist)-susceptible (APO-SUS) rat model and pharmacologically induced
mouse and rat models of schizophrenia, we found that MAM reduced gnawing stereotypy and climbing
behaviours induced by dopaminergic agents. This predicts antipsychotic activity. In line, MAM
antagonized apomorphine-induced c-Fos and NPAS4 mRNA levels in post-mortem brain nucleus accumbens
and dorsolateral striatum of APO-SUS rats. Furthermore, phencyclidine (PCP, an NMDA receptor
antagonist) and 2,5-Dimethoxy-4-iodoamphetamine (DOI, a 5HT2A/2C receptor agonist) induced
prepulse inhibition deficits, reflecting the positive symptoms of schizophrenia, which were rescued
by treatment with MAM and atypical antipsychotics alike. Post-mortem brain immunostaining
revealed that MAM blocked the strong activation of both PCP- and DOI-induced c-Fos immunoreactivity
in a number of cortical areas. Finally, during a 28-day subchronic treatment regime, MAM did
not induce weight gain, hyperglycemia, hyperlipidemia or hepato- and nephrotoxic effects, side effects
known to be induced by atypical antipsychotics. MAM also did not show any cataleptic effects. In
conclusion, its brain penetrability, the apparent absence of preclinical side effects, and its ability to antagonize
positive and cognitive symptoms associated with schizophrenia make MAM an exciting new
antipsychotic drug that deserves clinical testing.
Water-Mediated Synthesis, Antibacterial and Antioxidant Evaluation of
New Fused Pyrimido-pyrimidine and Pyrimido-purines Derived From
Nucleobases
Authors: Bouguessa Ichrak , Aber Mohamed , Khier Nawal , Dehamchia Mohamed , Bayou Samir , Régaïnia Zine
Published: July 2023

Introduction:

A simple and eco-friendly synthesis of novel substituted pyrimido[1,6-
a]pyrimidine, pyrimido[1,2-g]purine, and pyrimido[2,1-e]purine was accomplished by refluxing of nucleobases
(cytosine, adenine or guanine) and dibenzalacetone (DBA) in water using NaOH as a catalyst.

Methods:

The molecular structures of the resulting products were characterized by infrared spectroscopy
(FT-IR), mass spectrometry, and proton (1H) and carbon (13C) nuclear magnetic resonance (NMR).

Results:

The antibacterial activity of the newly fused heterocycles was assayed against the Gram-positive
bacterium Staphylococcus aureus (ATCC 6538) and Gram-negative Escherichia coli (ATCC 8737) using
gentamicin as a standard commercially available antibiotic

Conclusion:

as a standard commercially available antibiotic.
Conclusion: In addition, the antioxidant capacity was screened using the 2,2-diphenyl-2-picrylhydrazyl
hydrate (DPPH•) and the 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS+•)
radicals scavenging assay.
A Retrospective Study of the Safety and Effect of Co-administration of Glucose-lowering Medication and Bojungikgi-tang on Blood Glucose Level in Patients with Type 2 Diabetes Mellitus
Authors: Oh Seung-hyun , Jung Woo-nyoung , Song Mee-ryoung , Noh Ji-won , Ahn Young-min , Ahn Se-young , Lee Byung-cheol
Published: July 2023

Objective: This study aimed to assess the safety and effect on glucose level of Bojungikgi-tang in patients with type 2 diabetes mellitus.Methods: To review patients’ clinical characteristics and laboratory tests retrospectively, we investigated 15 hospitalized patients with type 2 diabetes mellitus who took Bojungikgi-tang at Kyung Hee University Korean Medical Hospital for at least one day between January 2012 and December 2022. The blood glucose levels, including fasting blood sugar (FBS), 2-hour postprandial glucose (PP2) levels, and glycated hemoglobin level, were collected to determine the effect of the Bojungikgi-tang on blood sugar changes. Furthermore, to evaluate the safety of Bojungikgi-tang, hepatic function and renal function tests were implemented, including aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, γ-glutamyltransferase, blood urea nitrogen, creatinine, and Modification of Diet in Renal Disease estimated glomerular filtration rate.Results: When Bojungikgi-tang and a standard treatment for diabetes were administered for patients with type 2 diabetes, it was confirmed that there were no statistically significant changes in FBS and PP2 levels in the analysis of each medication taken. There was no significant difference in the safety profile after taking Bojungikgi-tang.Conclusions: The combined administration of Bojungikgi-tang with standard hypoglycemic medication for patients with type 2 diabetes may not affect blood glucose levels and safety.
Research for the Development of an Integrated Medical System for Idiopathic Pulmonary Fibrosis: Based on an Analysis of Questionnaire Survey in Doctor and Patient Groups
Authors: Park Jiwon , Lee Beom-Joon , Shin Jeong-Won , Chin Su-Hyun , Yang Jung-Min , Kim Sooduk , Kim Kwan-Il , Choi Hye Sook , Jung Hee-Jae
Published: July 2023

Objective: In this study, a questionnaire survey was conducted for doctors and patients to obtain basic data necessary for the development of an integrated medical system for idiopathic pulmonary fibrosis (IPF).Methods: Questionnaires were developed separately for doctors and patients through an expert group meeting. The survey subjects were recruited online and offline, and finally, responses from 231 doctors and 59 patients were used for statistical analysis.Results: The most important parts in the treatment of IPF for both doctors and patients were the “improvement of respiratory symptoms,” “improvement of quality of life,” and “prevention of disease progression.” Antifibrotic agents were prescribed at a high rate, and 100% of the specialists in Western medicine (WM) and 45.8% of patients reported experiencing side effects. As for the additional payment costs that patients considered as affordable for an integrated medical system, “under 50,000 won (about 38$)” was reported the most in both doctor and patient groups. With regard to the reasons for their reluctance to recommend an integrated medical system for IPF, specialists in WM responded highly to “uncertain evidence for treatment effectiveness.” Regarding complementary and alternative medicine therapies that can be beneficial in patients with IPF, “lifestyle management,” “diet management,” “herb,” “relaxation therapy,” and “psychotherapy” were ranked high in both doctor and patient groups.Conclusions: In this study, a questionnaire survey on IPF was conducted to review actual treatment status, analyze shortcomings, and identify considerations for the development of an integrated medical system for IPF in the future.
Effect of Herbal Medicines on Preventing Restenosis After Percutaneous Coronary Intervention: A Systematic Review and Meta-analysis of Randomized Controlled Trials
Authors: Hur Nayeon , Ahn Subin , Kim Hongjun , Jang Insoo
Published: July 2023

Objective: This study was conducted to investigate the preventive effect of herbal medicines on restenosis after percutaneous coronary intervention (PCI) by reviewing randomized controlled trials (RCTs).Methods: RCTs were searched for herbal medicine treatment after PCI using eight online databases (PubMed, CNKI, Wanfang, J-STAGE, OASIS, ScienceON, KTKP, and KISS). Studies that confirmed restenosis through coronary angiography at follow-up were selected according to the inclusion and exclusion criteria. The primary outcome was the restenosis rate, and the secondary outcome was the angina recurrence rate. Data were extracted from the final selected studies according to the research methodology and then analyzed with Review Manager 5.4.1. Study quality was assessed using Cochrane’s risk-of-bias (RoB) tool.Results: Of the 252 papers obtained through the primary search, nine studies that met the selection criteria were finally selected. In these nine studies, herbal medicine combined with western medicine was used for the experimental group, and western medicine treatment was used alone for the control group. The meta-analysis result revealed that the restenosis rate and angina recurrence rate were significantly lower in the experimental group than in the control group (RR=0.34, 95% CI: 0.22-0.53, p<0.00001, I²=0% and RR=0.47, 95% CI: 0.29-0.78, p=0.004, I²=0%, respectively). Furthermore, the quality of studies assessed by Cochrane’s RoB was low.Conclusions: This study showed that the combined treatment of herbal medicine and western medicine was effective in preventing restenosis and angina after PCI. As the number of papers included in this study was small, a large number of high-quality clinical studies should be considered in the future.
A Case Report of Osuyubujaijung-tang on the Recurrence of Breast Cancer in a Patient Complaining of Coldness and Dizziness
Authors: Chung U-ryeong , Hong Soo-hwa , Jang Han-sol , Jung Seung-hyun
Published: July 2023

A 63-year-old female patient was admitted to the hospital with complaints of coldness in the head and right femur, persistent vertigo, dry mouth, vexation, general weakness, anorexia, and hot flashes. After being diagnosed with left-sided ductal carcinoma in 2017 and undergoing partial resection, she was recommended radiotherapy, chemotherapy, and hormone therapy, but she refused and did not receive any treatment. At a checkup in November 2022, the left-sided invasive ductal carcinoma recurred. She underwent partial resection and was scheduled to receive radiotherapy two months later. During hospitalization, the patient received traditional Korean medicine treatment, including Korean herbal medicine (Osuyubujaijung-tang), acupuncture, moxibustion, and cupping. Subjective symptom changes were evaluated daily. At discharge, the patient’s symptoms improved, and her condition varied with the presence of Aconitum carmichaelii. This study suggests that “Osuyubujaijung-tang” cared the coldness and vertigo caused by “Han-dam (寒痰)” in patients with Soeumin visceral syncope pattern (少陰人 臟厥症) and that A. carmichaelii played a major role in this outcome.