bluuebunny/crossref_metadata_2025 huggingface dataset:
HER3-Receptor-Mediated STAT3 Activation Plays a Central Role in Adaptive Resistance toward Vemurafenib in Melanoma
Authors: Hüser Laura , Kokkaleniou Marianthi-Maria , Granados Karol , Dworacek Jennifer , Federico Aniello , Vierthaler Marlene , Novak Daniel , Arkhypov Ihor , Hielscher Thomas , Umansky Viktor , Altevogt Peter , Utikal Jochen
Published: December 2020

Melanoma is an aggressive form of skin cancer that is often characterized by activating mutations in the Mitogen-Activated Protein (MAP) kinase pathway, causing hyperproliferation of the cancer cells. Thus, inhibitors targeting this pathway were developed. These inhibitors are initially very effective, but the occurrence of resistance eventually leads to a failure of the therapy and is the major obstacle for clinical success. Therefore, investigating the mechanisms causing resistance and discovering ways to overcome them is essential for the success of therapy. Here, we observed that treatment of melanoma cells with the B-Raf Proto-Oncogene, Serine/Threonine Kinase (BRAF) inhibitor vemurafenib caused an increased cell surface expression and activation of human epidermal growth factor receptor 3 (HER3) by shed ligands. HER3 promoted the activation of signal transducer and activator of transcription 3 (STAT3) resulting in upregulation of the STAT3 target gene SRY-Box Transcription Factor 2 (SOX2) and survival of the cancer cells. Pharmacological blocking of HER led to a diminished STAT3 activation and increased sensitivity toward vemurafenib. Moreover, HER blocking sensitized vemurafenib-resistant cells to drug treatment. We conclude that the inhibition of the STAT3 upstream regulator HER might help to overcome melanoma therapy resistance toward targeted therapies.
Ubiquitination and Ubiquitin-Like Modifications in Multiple Myeloma: Biology and Therapy
Authors: Wirth Matthias , Schick Markus , Keller Ulrich , Krönke Jan
Published: December 2020

Multiple myeloma is a genetically heterogeneous plasma cell malignancy characterized by organ damage and a massive production of (in-)complete monoclonal antibodies. Coping with protein homeostasis and post-translational regulation is therefore essential for multiple myeloma cells to survive. Furthermore, post-translational modifications such as ubiquitination and SUMOylation play key roles in essential pathways in multiple myeloma, including NFκB signaling, epigenetic regulation, as well as DNA damage repair. Drugs modulating the ubiquitin–proteasome system, such as proteasome inhibitors and thalidomide analogs, are approved and highly effective drugs in multiple myeloma. In this review, we focus on ubiquitin and ubiquitin-like modifications in the biology and current developments of new treatments for multiple myeloma.
A Novel Platform to Test In Vivo Single Gene Dependencies in t(8,21) and t(15,17) AML Confirms Zeb2 as Leukemia Target
Authors: De Conti Giulia , Gruszka Alicja M. , Valli Debora , Cammarata Andrea Umberto , Righi Matteo , Mazza Massimiliano , Pelicci Pier Giuseppe
Published: December 2020

The increased usage of high-throughput technologies in cancer research, including genetic and drug screens, generates large sets of candidate targets that need to be functionally validated for their roles in tumor development. Thus, reliable and robust in vivo model systems are needed to perform reverse genetic experiments. Ideally, these models should allow for a conditional silencing of the target and an unambiguous identification of engineered cancer cells. Here, we present a platform consisting of: (i) t(8;21) and t(15;17) driven acute myeloid leukemia (AML) transgenic mice with constitutive expression of green fluorescent protein (GFP) and inducible expression of Cre recombinase, and (ii) REX, a modified pSico lentiviral vector for inducible shRNA expression and red fluorescent protein (RFP) as a selection marker. In this system, leukemic cells from transgenic mice are transduced with REX, flow sorted, and transplanted into syngeneic hosts. Gene interference is induced in established tumors by tamoxifen treatment. Dual-color cell fluorescence guides the in vivo identification of shRNA interfered AML cells, monitoring engraftment and disease progression. We tested the platform by inducing knockdown of Zeb2, a gene upregulated by AML1-ETO and PML-RARα oncogenes in pre-leukemic hematopoietic stem cell compartment, and observed a significant delay in leukemia onset. This proves the power and utility of the platform and confirms Zeb2 contribution to the pathogenesis of AML.
GAS2L1 Is a Potential Biomarker of Circulating Tumor Cells in Pancreatic Cancer
Authors: Zhu Lei , Kan Ke-Jia , Grün Johanna L. , Hissa Barbara , Yang Cui , Győrffy Balázs , Loges Sonja , Reißfelder Christoph , Schölch Sebastian
Published: December 2020

Pancreatic cancer is a malignant disease with high mortality and a dismal prognosis. Circulating tumor cell (CTC) detection and characterization have emerged as essential techniques for early detection, prognostication, and liquid biopsy in many solid malignancies. Unfortunately, due to the low EPCAM expression in pancreatic cancer CTCs, no specific marker is available to identify and isolate this rare cell population. This study analyzed single-cell RNA sequencing profiles of pancreatic CTCs from a genetically engineered mouse model (GEMM) and pancreatic cancer patients. Through dimensionality reduction analysis, murine pancreatic CTCs were grouped into three clusters with different biological functions. CLIC4 and GAS2L1 were shown to be overexpressed in pancreatic CTCs in comparison with peripheral blood mononuclear cells (PBMCs). Further analyses of PBMCs and RNA-sequencing datasets of enriched pancreatic CTCs were used to validate the overexpression of GAS2L1 in pancreatic CTCs. A combinatorial approach using both GAS2L1 and EPCAM expression leads to an increased detection rate of CTCs in PDAC in both GEMM and patient samples. GAS2L1 is thus proposed as a novel biomarker of pancreatic cancer CTCs.
A New Landscape for Systemic Pharmacotherapy of Recurrent Glioblastoma?
Authors: Lombardi Giuseppe , Idbaih Ahmed , Le Rhun Emilie , Preusser Matthias , Zagonel Vittorina , French Pim
Published: December 2020

Glioblastoma is the most common and aggressive primary malignant brain tumor in adult patients [...]
Pathogenic Genome Signatures That Damage Motor Neurons in Amyotrophic Lateral Sclerosis
Authors: Yousefian-Jazi Ali , Seol YunHee , Kim Jieun , Ryu Hannah L. , Lee Junghee , Ryu Hoon
Published: December 2020

Amyotrophic lateral sclerosis (ALS) is the most frequent motor neuron disease and a neurodegenerative disorder, affecting the upper and/or lower motor neurons. Notably, it invariably leads to death within a few years of onset. Although most ALS cases are sporadic, familial amyotrophic lateral sclerosis (fALS) forms 10% of the cases. In 1993, the first causative gene (SOD1) of fALS was identified. With rapid advances in genetics, over fifty potentially causative or disease-modifying genes have been found in ALS so far. Accordingly, routine diagnostic tests should encompass the oldest and most frequently mutated ALS genes as well as several new important genetic variants in ALS. Herein, we discuss current literatures on the four newly identified ALS-associated genes (CYLD, S1R, GLT8D1, and KIF5A) and the previously well-known ALS genes including SOD1, TARDBP, FUS, and C9orf72. Moreover, we review the pathogenic implications and disease mechanisms of these genes. Elucidation of the cellular and molecular functions of the mutated genes will bring substantial insights for the development of therapeutic approaches to treat ALS.
Publication Trends of Pediatric and Adult Randomized Controlled Trials in General Medical Journals, 2005–2018: A Citation Analysis
Authors: Groff Michael L. , Offringa Martin , Emdin Abby , Mahood Quenby , Parkin Patricia C. , Cohen Eyal
Published: December 2020

Policy has been developed to promote the conduct of high-quality pediatric randomized controlled trials (RCTs). Whether these strategies have influenced publication trends in high-impact journals is unknown. We aim to evaluate characteristics, citation patterns, and publication trends of pediatric RCTs published in general medical journals (GMJs) compared with adult RCTs over a 13-year period. Studies were identified using Medline, and impact metrics were collected from Web of Science and Scopus. All RCTs published from 2005–2018 in 7 GMJs with the highest impact factors were identified for analysis. A random sample of matched pediatric and adult RCTs were assessed for publication characteristics, academic and non-academic citation. Citations were counted from publication until June 2019. Among 4146 RCTs, 2794 (67.3%) enrolled adults, 591 (14.2%) enrolled children, and 761 RCTs (18.3%) enrolled adult and pediatric patients. Adult RCTs published in GMJs grew by 5.1 publications per year (95% CI: 3.3–6.9), while the number of pediatric RCTs did not show significant change (−0.4 RCTs/year, 95% CI: −1.4–0.6). Adult RCTs were cited more than pediatric RCTs (median(IQR): 29.9 (68.5–462.8) citations/year vs. 13.2 (6.8–24.9) citations/year; p < 0.001); however, social media attention was similar (median(IQR) Altmetric Attention Score: 37 (13.75–133.8) vs. 26 (6.2–107.5); p = 0.25). Despite policies which may facilitate conduct of pediatric RCTs, the publishing gap in high-impact GMJs is widening.
Impacts of Agroclimatic Variability on Maize Production in the Setsoto Municipality in the Free State Province, South Africa
Authors: Hadisu Bello Abubakar , Scholes Mary , Newete Solomon W.
Published: December 2020

The majority of people in South Africa eat maize, which is grown as a rain-fed crop in the summer rainfall areas of the country, as their staple food. The country is usually food secure except in drought years, which are expected to increase in severity and frequency. This study investigated the impacts of rainfall and minimum and maximum temperatures on maize yield in the Setsoto municipality of the Free State province of South Africa from 1985 to 2016. The variation of the agroclimatic variables, including the Palmer stress diversity index (PSDI), was investigated over the growing period (Oct–Apr) which varied across the four target stations (Clocolan, Senekal, Marquard and Ficksburg). The highest coefficients of variance (CV) recorded for the minimum and maximum temperatures and rainfall were 16.2%, 6.2% and 29% during the growing period. Non-parametric Mann Kendal and Sen’s slope estimator were used for the trend analysis. The result showed significant positive trends in minimum temperature across the stations except for Clocolan where a negative trend of 0.2 to 0.12 °C year−1 was observed. The maximum temperature increased significantly across all the stations by 0.04–0.05 °C year−1 during the growing period. The temperature effects were most noticeable in the months of November and February when leaf initiation and kernel filling occur, respectively. The changes in rainfall were significant only in Ficksburg in the month of January with a value of 2.34 mm year−1. Nevertheless, the rainfall showed a strong positive correlation with yield (r 0.46, p = < 0.05). The overall variation in maize production is explained by the contribution of the agroclimatic parameters; the minimum temperature (R2 0.13–0.152), maximum temperature (R2 0.214–0.432) and rainfall (R2 0.17–0.473) for the growing period across the stations during the study period. The PSDI showed dry years and wet years but with most of the years recording close to normal rainfall. An increase in both the minimum and maximum temperatures over time will have a negative impact on crop yield.
One-Step Reinforcement and Deacidification of Paper Documents: Application of Lewis Base—Chitosan Nanoparticle Coatings and Analytical Characterization
Authors: Jia Zhihui , Yang Chun , Zhao Fangnan , Chao Xiaolian , Li Yuhu , Xing Huiping
Published: December 2020

To delay acidification and deterioration during natural aging, deacidification and reinforcement of paper manuscripts have been the most important technologies to prolong the life of objects. Herein, a novel approach for the conservation of paper manuscripts is proposed using chitosan nanoparticles as Lewis base that leads to both deacidification and strengthening of paper in one-step. Chitosan nanoparticles were prepared through physical ball grinding method and characterized via scanning electron microscopy (SEM), X-ray diffraction (XRD), laser particle size analyzer (LPSA), Fourier transform infrared spectroscopy (FTIR), and atomic force microscope (AFM). To evaluate the resistance of chitosan nanoparticle coating, the mechanical properties of paper after artificial aging were evaluated using dry heat and hygrothermal accelerated aging methods. The SEM, EDX, and X-ray Photoelectron Spectroscopy (XPS) were used to analyze the interaction mechanism between chitosan and Shuxuan paper. The results show that the coated paper had superior durability with respect to pH, tensile strength, and folding endurance. There was a presence of protonated amines in the form of ammonium salts due to ionic bindings with free H+ in the acidified paper, and the remaining –NH2 could be used as a base reserve. Finally, the resulting coated papers displayed good antibacterial properties.
Special Issue “Exercise-Induced Facial Rejuvenation and Orofacial Strength and Function”
Authors: Abe Takashi
Published: December 2020

The desire to stay young and beautiful forever is a common aspiration for everyone [...]
Microstructural and Hardness Evolution in a Duplex Stainless Steel Processed by High-Pressure Torsion
Authors: Ma Ming , Ding Hua , Huang Yi , Tian Cheng Wei , Langdon Terence G.
Published: December 2020

The duplex stainless steel 2205, designated DSS2205 and having a duplex structure comprising ferrite and austenite phases, was processed by high-pressure torsion (HPT) and the microstructural and hardness evolutions were investigated after various HPT revolutions and at different positions within the specimens. The results show that the grain refinement induced by severe deformation processing is different in the ferrite and austenite phases such that the ferrite grains are refined via dislocation subdivision, whereas grain refinement in the austenite phase depends mainly on the interaction of dislocations and twin boundaries at relatively low strains. When the numbers of revolutions increases, the grain refinement in austenite restricts the occurrence of deformation twinning so that dislocation slip becomes dominant. During HPT processing, the effect of the phase boundaries on the mechanical properties of the alloy is very significant. The results show the average width between two adjacent phases and the hardness of the alloy are generally consistent with the classical Hall–Petch relationship.
Dataset on the Effects of Different Pre-Harvest Factors on the Metabolomics Profile of Lettuce (Lactuca sativa L.) Leaves
Authors: Corrado Giandomenico , Lucini Luigi , Miras-Moreno Begoña , Zhang Leilei , Senizza Biancamaria , Basile Boris , Rouphael Youssef
Published: December 2020

The study of the relationship between cultivated plants and environmental factors can provide information ranging from a deeper understanding of the plant biological system to the development of more effective management strategies for improving yield, quality, and sustainability of the produce. In this article, we present a comprehensive metabolomics dataset of two phytochemically divergent lettuce (Lactuca sativa L.) butterhead varieties under different growing conditions. Plants were cultivated in hydroponics in a growth chamber with ambient control. The pre-harvest factors that were independently investigated were light intensity (two levels), the ionic strength of the nutrient solutions (three levels), and the molar ratio of three macroelements (K, Mg, and Ca) in the nutrient solution (three levels). We used an untargeted, mass-spectrometry-based approach to characterize the metabolomics profiles of leaves harvested 19 days after transplant. The data revealed the ample impact on both primary and secondary metabolism and its range of variation. Moreover, our dataset is useful for uncovering the complex effects of the genotype, the environmental factor(s), and their interaction, which may deserve further investigation.
Prognostic Implications of Epilepsy Onset Age According to Relapse Pattern in Patients with Four-Year Remission
Authors: Park Soochul , Lee Myeongjee
Published: December 2020

A total of 472 epilepsy patients with a 4-year remission period were divided into 10-year age groups according to age of onset. The relapse patterns during at least 3 years of follow-up were classified as early relapse (ER), late relapse (LR), and seizure-free (SF). The remission probability and multiplicity of prognostic factors were evaluated using univariate and multivariate multinomial logistic analyses. The weighted risk score based on odd ratios (ORs) was used for comparisons of the relative risk of relapse between groups. The group with onset in their 20s had the lowest remission probability among the groups. The risks of relapse in the LR patients and the relative weighted risk score of ER patients in the group with onset in their 20s were 3.11 and 19.44, respectively, which was the highest risk among the age groups. Patients without remission within 1 year had the highest relapse risk, with an OR of 7.18 in ER patients. The OR of relapse in patients with >10 generalized tonic–clonic (GTC) seizures was the second most important prognostic factor in LR patients. The distinct risk and corresponding prognostic factors in LR and ER patients reflected inherent differences between these relapse patterns.
Can MRI Biomarkers Predict Triple-Negative Breast Cancer?
Authors: Moffa Giuliana , Galati Francesca , Collalunga Emmanuel , Rizzo Veronica , Kripa Endi , D’Amati Giulia , Pediconi Federica
Published: December 2020

The purpose of this study was to investigate MRI features of triple-negative breast cancer (TNBC) compared with non-TNBC, to predict histopathological results. In the study, 26 patients with TNBC and 24 with non-TNBC who underwent multiparametric MRI of the breast on a 3 T magnet over a 10-months period were retrospectively recruited. MR imaging sets were evaluated by two experienced breast radiologists in consensus and classified according to the 2013 American College of Radiology (ACR) BI-RADS lexicon. The comparison between the two groups was performed using the Chi-square test and followed by logistic regression analyses. We found that 92% of tumors presented as mass enhancements (p = 0.192). 41.7% of TNBC and 86.4% of non-TNBC had irregular shape (p = 0.005); 58.3% of TNBC showed circumscribed margins, compared to 9.1% of non-TNBC masses (p = 0.001); 75% of TNBC and 9.1% of non-TNBC showed rim enhancement (p < 0.001). Intralesional necrosis was significantly associated with TNBC (p = 0.016). Rim enhancement and intralesional necrosis risulted to be positive predictors at univariate analysis (OR = 29.86, and 8.10, respectively) and the multivariate analysis confirmed that rim enhancement is independently associated with TNBC (OR = 33.08). The mean ADC values were significantly higher for TNBC (p = 0.011). In conclusion, TNBC is associated with specific MRI features that can be possible predictors of pathological results, with a consequent prognostic value.
Neurocognitive Functioning and Suicidal Behavior in Violent Offenders with Schizophrenia Spectrum Disorders
Authors: Sánchez-Sansegundo Miriam , Portilla-Tamarit Irene , Rubio-Aparicio María , Albaladejo-Blazquez Natalia , Ruiz-Robledillo Nicolás , Ferrer-Cascales Rosario , Zaragoza-Martí Ana
Published: December 2020

Suicide is one of the main premature causes of death in patients with schizophrenia. However, little is known about the relationship between neurocognitive functioning and suicidality in violent offenders with schizophrenia who have been sentenced to psychiatric treatment after committing violent crimes. We examined the neurocognitive functioning of a sample of 61 violent offenders, most of them murderers with schizophrenia who were classified as suicide attempters (n = 26) and non-attempters (n = 35). We compared the neurocognitive functioning of both groups using a neuropsychological battery. Suicide attempters showed similar performance to non-attempters in a neuropsychological test across all domains of cognitive functioning, memory, attention, verbal fluency, and executive functioning. However, after controlling for demographic and clinical variables, suicide attempters performed better than non-attempters in two planning-related tasks: the Tower of London (p < 0.01) and the Zoo Map (p < 0.01). Suicide attempters were also characterized as having more family histories of suicidality and as displaying more depressive symptoms and negative symptoms of psychopathology on the Positive and Negative Syndrome Scale (PANSS) scale. These results suggest that suicide attempters have a greater ability to formulate plans and initiate goals directed at making a suicide attempt.
Thermal Simulation of Phase Change Material for Cooling of a Lithium-Ion Battery Pack
Authors: Madani Seyed Saeed , Schaltz Erik , Kær Søren Knudsen
Published: December 2020

A new heat transfer enhancement approach was proposed for the cooling system of lithium-ion batteries. A three-dimensional numerical simulation of the passive thermal management system for a battery pack was accomplished by employing ANSYS Fluent (Canonsburg, PA, USA). Phase change material was used for the thermal management of lithium-ion battery modules and as the heat transmission source to decrease battery temperature in fast charging and discharge conditions. Constant current charge and discharge were applied to lithium-ion battery modules. In the experimental part of the research, an isothermal battery calorimeter was used to determine the heat dissipation of lithium-ion batteries. Thermal performance was simulated for the presence of phase change material composites. Simulation outcomes demonstrate that phase change material cooling considerably decreases the lithium-ion battery temperature increase during fast charging and discharging conditions use. The greatest temperature at the end of 9 C, 7 C, 5 C, and 3 C charges and discharges were approximately 49.7, 44.6, 38.4, and 33.1 °C, respectively, demonstrating satisfactory performance in lithium-ion battery thermal homogeneity of the passive thermal management system.
Improving Classification Accuracy of Hand Gesture Recognition Based on 60 GHz FMCW Radar with Deep Learning Domain Adaptation
Authors: Lee Hyo Ryun , Park Jihun , Suh Young-Joo
Published: December 2020

With the recent development of small radars with high resolution, various human–computer interaction (HCI) applications using them have been developed. In particular, a method of applying a user’s hand gesture recognition using a short-range radar to an electronic device is being actively studied. In general, the time delay and Doppler shift characteristics that occur when a transmitted signal that is reflected off an object returns are classified through deep learning to recognize the motion. However, the main obstacle in the commercialization of radar-based hand gesture recognition is that even for the same type of hand gesture, recognition accuracy is degraded due to a slight difference in movement for each individual user. To solve this problem, in this paper, the domain adaptation is applied to hand gesture recognition to minimize the differences among users’ gesture information in the learning and the use stage. To verify the effectiveness of domain adaptation, a domain discriminator that cheats the classifier was applied to a deep learning network with a convolutional neural network (CNN) structure. Seven different hand gesture data were collected for 10 participants and used for learning, and the hand gestures of 10 users that were not included in the training data were input to confirm the recognition accuracy of an average of 98.8%.
Optimization Method for the Evaluation of Convective Heat and Mass Transfer Effective Coefficients and Energy Sources in Drying Processes
Authors: Stasiak Marcin , Musielak Grzegorz , Mierzwa Dominik
Published: December 2020

A new optimization method for the assessment of the coefficients existing in a model of drying kinetics is developed and presented in this article. This method consists of matching the drying kinetics resulting from the mathematical model with the drying kinetics resulting from the experiments. Both the heat and mass transfer coefficients, the critical relative humidity, and the additional ultrasound energy (heat) source are included in the optimization procedure. The Adams–Bashforth multistep method of solving nonlinear ordinary differential equations is used. The inverse problem of model parameter estimation is solved by the Rosenbrock optimization method. The methodology is illustrated by the example of the ultrasound-assisted convective drying of apple and carrot. A high level of agreement between the results obtained experimentally and numerically was found. The obtained results confirmed the great influence of ultrasound on the drying kinetics. It was found that ultrasound application improved the mass transfer by 20–80% and heat transfer by 30–90%. It was also found that the heating effect caused by the ultrasound’s absorption was very small, with a value below 1%.
Optimized Design of 1 MHz Intermediate Bus Converter Using GaN HEMT for Aerospace Applications
Authors: Maset Enrique , Ejea Juan Bta. , Ferreres Agustín , Lizán José Luis , Blanes Jose Manuel , Sanchis-Kilders Esteban , Garrigós Ausias
Published: December 2020

This paper presents the possibility of using Gallium Nitride (GaN) high-electron-mobility transistors (HEMTs) instead of the conventional silicon metal oxide semiconductor field effect transistor (MOSFET) to implement a high-frequency intermediate bus converter (IBC) as part of a typical distributed power architecture used in a space power application. The results show that processing the power at greater frequencies is possible with a reduction in mass and without impacting the system efficiency. The proposed solution was experimentally validated by the implementation of a 1 MHz zero-voltage and zero-current switching (ZVZCS) current-fed half-bridge converter with synchronous rectification compared with the same converter using silicon as the standard technology on power switches and working at 100 kHz. In conclusion, the replacement of silicon (Si) transistors by GaN HEMTs is feasible, and GaN HEMTs are promising next-generation devices in the power electronics field and can coexist with silicon semiconductors, mainly in some radiation-intensive environments, such as power space converters. The best physical properties of GaN HEMTs, such as inherent radiation hardness, low on resistance and parasitic capacitances, allow them to switch at higher frequencies with high efficiency achieving higher power density. We present an optimized design procedure to guaranty the zero-voltage switching condition that enables the power density to be increased without a penalization of the efficiency.
Review of Process Modeling of Solid-Fuel Thermal Power Plants for Flexible and Off-Design Operation
Authors: Avagianos Ioannis , Rakopoulos Dimitrios , Karellas Sotirios , Kakaras Emmanouil
Published: December 2020

Since the widespread deployment of non-dispatchable, intermittent, and highly variable power production from renewable energy sources (RES), the demand for flexible power production has been steadily growing. As new-built dispatchable power plants have not been very quickly adapted to the emerging flexible operation, this task has been addressed by existing plants as well. Existing solid-fuel thermal power plants have undergone an extensive study to increase their flexible operation. Thermodynamic process-modeling tools have been extensively used for plant modeling. Steady- and transient-state simulations have been performed under various operating regimes, supplying valuable results for efficient power-plant operation. Flexibility aspects regarding low-load operation and steady operational conditions are mostly investigated with steady-state simulations. Flexibility aspects related to variation over time such as ramping rates are investigated with transient simulations. The off-design operation is mainly attributed to the existing fleet of power plants, struggling to balance between their former operational schemes as base and/or medium-load plants. However, off-design operation is also considered for new plants in the design phase and is included as a simulation aspect. Process modeling turns out to be a proven tool for calculating plant flexibility and predicting extreme operating conditions, defining further steps for a new operational scheme, drafting accident mitigation control procedures or, furthermore, provisioning more complex and cross-field future tasks. A review of the off-design aspect as a simulation approach is undertaken and presented in this work. Finally, challenges and future perspectives for this aspect of solid-fuel thermal power plants are discussed.
The Impact of Purchasing Group on the Profitability of Companies Operating in the Renewable Energy Sector—The Case of Poland
Authors: Zimon Grzegorz , Zimon Dominik
Published: December 2020

Purchasing groups are multi-entity organisations that operate in practically every sector. Their greatest role is to improve the financial situation of small and medium enterprises (SMEs). In the case of enterprises operating in the sectors that are just developing, especially those belonging to SMEs, it is particularly difficult to obtain favourable financial results. In Poland, such sectors include, among others, renewable energy, which is slowly developing, but has not yet reached the size set in climate policy. The purpose of the paper is to present how functioning under group purchasing organisations (GPOs) affects the profitability of companies related to the renewable energy sector. For the example of the largest industry purchasing groups, the analysis showed that high profitability results were achieved by enterprises operating in them compared to entities operating independently in the market. In total, 71 enterprises operating in the sector related to the renewable energy sector in Poland were analysed. The enterprises were divided into units operating in the purchasing groups of 46 enterprises, and those that operate independently in the market of 25 enterprises. The research period covered the years 2016–2019. The analysis showed that the scale effect allowed companies to increase profits and had a positive effect on their financial liquidity. The research proved that in the case of commercial enterprises operating in the sector related to renewable energy, functioning within the industry purchasing group allowed high profitability and financial liquidity. There is a dependency that the liquidity increases with a growth in profitability.
Design and Implementation of Model Predictive Control Based PID Controller for Industrial Applications
Authors: Aboelhassan Ahmed , Abdelgeliel M. , Zakzouk Ezz Eldin , Galea Michael
Published: December 2020

Advanced control approaches are essential for industrial processes to enhance system performance and increase the production rate. Model Predictive Control (MPC) is considered as one of the promising advanced control algorithms. It is suitable for several industrial applications for its ability to handle system constraints. However, it is not widely implemented in the industrial field as most field engineers are not familiar with the advanced techniques conceptual structure, the relation between the parameter settings and control system actions. Conversely, the Proportional Integral Derivative (PID) controller is a common industrial controller known for its simplicity and robustness. Adapting the parameters of the PID considering system constraints is a challenging task. Both controllers, MPC and PID, merged in a hierarchical structure in this work to improve the industrial processes performance considering the operational constraints. The proposed control system is simulated and implemented on a three-tank benchmark system as a Multi-Input Multi-Output (MIMO) system. Since the main industrial goal of the proposed configuration is to be easily implemented using the available automation technology, PID controller is implemented in a PLC (Programable Logic Controller) controller as a lower controller level, while MPC controller and the adaptation mechanism are implemented within a SCADA (Supervisory Control And Data Acquisition) system as a higher controller level.
Possibilities of Speciation in the Central Sandy Steppe, Woody Steppe Area of the Carpathian Basin through the Example of Festuca Taxa
Authors: Penksza Károly , Csík Attila , Filep Anna Fruzsina , Saláta Dénes , Pápay Gergely , Kovács László , Varga Kristina , Pauk János , Lantos Csaba , Lisztes-Szabó Zsuzsa
Published: December 2020

Research Highlights: We examined the vegetation appearing in forest-steppes in the Pannon region. In the present survey taxonomical relations of the dominant Festuca species were examined. Background and Objectives: After deforestation and shrubcutting bare soil patches exposed to anthropogenous effects provided an opportunity for new vegetation to form. Materials and Methods: Inflorescence parameters and micromorphological characters of the leaves were examined in a new taxon and compared with two, presumably closely related, species of the genus Festuca L. Festuca tomanii Korneck & T.Gregor, with silvery leaf surface, Festuca vaginata W. K. and Festuca pseudovaginata Penksza were compared based on 24 traits of the inflorescence and their leaf anatomy studied on leaf cross-sections. Moreover, leaf micromorphological features were compared using a stereomicroscope, a scanning electron microscope completed with Energy Dispersive X-Ray Spectroscopy measurements and phytolith analysis method to establish the taxonomic applications of the micromorphological characters of the epidermis. Results: The awns of the lemma of Festuca tomanii were shown to be longer than those of the two other species. Festuca vaginata and Festuca pseudovaginata specimen showed low variability in inflorescence parameters but inflorescence characters were not uniform because the panicle of Festuca tomanii individuals was found to be bigger in the northern part than the panicles originating from the southern part of the sampled area. The phytolith assemblages of the Festuca pseudovaginata and Festuca tomanii differ from the Festuca vaginata in the abundance of ELONGATE SINUATE phytolith morphotype. Conclusions: we confirmed the appearance of F. vaginata in natural grasslands and discovered new occurrences of F. pseudovaginata and F. tomanii. F. pseudovaginata inhabits only the Pannon region, we found endemic and natural stands of it, but in its secondary habitats it was confirmed as a completely new species. Furthermore, taxa of disturbed vegetations are currently being examined. These habitats are potential hotspots of speciation.
Gene Set Subtraction Reveals 633 Candidate Genes for Bamboo Culm Wall Thickening
Authors: Hu Yaping , Zhou Jie , Yu Zhaoyan , Li Jiajia , Cai Jinfeng , Wang Guibin , Guo Qirong
Published: December 2020

Abundant research has been conducted on the physiological, biochemical, and anatomical aspects of bamboo culm wall thickening, but its molecular mechanism has not yet been investigated. In this study, we performed whole-genome resequencing of Phyllostachys edulis ‘Pachyloen’, Phyllostachys nidularia f. farcta, Phyllostachys heteroclada f. solida with significantly thicker culm walls, and Schizostachyum dumetorum var. xinwuense with extremely thin culm walls. Moreover, we pioneered the innovative use of gene set subtraction to explore candidate genes that regulate bamboo culm wall thickening. A candidate gene set, containing 633 genes, was obtained by eliminating shared genes that help maintain physiological processes after alignment with the P. edulis reference genome. Starch and sucrose, oxidative phosphorylation, and ribosome were the three most important pathways enriched by differentially expressed genes. Although it cannot be used for hyperfine localization of bamboo wall thickness-regulatory genes, gene set reduction narrows down the range of candidate genes at minimal cost and provides new clues for the application of bioinformatics in plant research.
Observations on the Malting of Ancient Wheats: Einkorn, Emmer and Spelt
Authors: Fujita Alice , Simsek Senay , Schwarz Paul B.
Published: December 2020

There have been tremendous marketing efforts and consumer interest in the so-called ancient grains. Einkorn, emmer and spelt, which are sometimes referred to as ancient wheats, are frequently included in this category, and have gained some attention among brewers. The objective of the current study was to compare the malting behavior and quality of einkorn, emmer and spelt cultivars obtained from the same growing environment. Aside from standard malt quality traits, the levels of β-amylase, protease, xylanase, wort arabinoxylans and wort phenolic acids were measured. While protein levels of the samples were higher (11.4–14.0%) than normally selected for wheat malt, the results indicated that malts of acceptable quality in terms of extract and amylolytic activity can be prepared from the three grain types. However, the ideal malting protocol will likely differ between the grains. The kernels of einkorn are significantly smaller, and steep hydration and malt modification are quicker. In terms of potential health benefits from antioxidant capacity and dietary fiber, wort from einkorn trended to higher levels of free and conjugated ferulic acid, as well as high-molecular-weight arabinoxylan.
Control of Penicillium glabrum by Indigenous Antagonistic Yeast from Vineyards
Authors: Cabañas Catalina M. , Hernández Alejandro , Martínez Ana , Tejero Paula , Vázquez-Hernández María , Martín Alberto , Ruiz-Moyano Santiago
Published: December 2020

Biocontrol is one of the most promising alternatives to synthetic fungicides for food preservation. Botrytis cinerea, Alternaria alternata, and Aspergillus section Nigri are the most concerning pathogens for grape development. However, frequently, other species, such as Penicillium glabrum in this study, are predominant in spoiled bunches. In this work, 54 native yeasts from vineyards were screened by direct confrontation in potato dextrose agar plates as antagonists against P. glabrum. Isolates of Pichia terricola, Aureobasidium pullulans, and Zygoascus meyerae were selected for their antagonist activity in vitro, plus isolates of Pichia kudriavzevii, Hormonema viticola, and Hanseniaspora uvarum were used as negative controls. However, in vivo, confrontations in wounded grapes showed disagreement with direct confrontation in vitro. P. terricola, P. kudriavzevii, H. viticola, Z. meyerae, and H. uvarum significantly reduced the incidence of P. glabrum on grapes. Nevertheless, P. terricola, H. viticola, and H. uvarum themselves spoiled the wounded grapes. Inhibitions were associated with different mechanisms such as the production of volatile organic compounds (VOCs), lytic enzymes, biofilm formation, and competition for nutrients. The isolates of P. kudriavzevii L18 (a producer of antifungal VOCs which completely inhibited the incidence of P. glabrum) and Z. meyerae L29 (with pectinase, chitinase and β-glucanase activity and biofilm formation which reduced 70% of the incidence of P. glabrum) are proposed as suitable biocontrol agents against P. glabrum.
Macroelements and Trace Elements Content in Brine-Canned Mackerel (Scomber colias) Subjected to High-Pressure Processing and Frozen Storage
Authors: Prego Ricardo , Vázquez Manuel , Cobelo-García Antonio , Aubourg Santiago P.
Published: December 2020

This study analysed the effect of prior high-pressure processing (HPP; 200–600 MPa, 2 min), freezing (−30 °C, 48 h), and frozen storage (−18 °C, 6 months) on the macroelement and trace element content in brine-canned mackerel (Scomber colias). Most elements (Na, Ca, Ba, Mn, Fe, Cu, Cd, Sn, As, S, and Se) showed an increased (p < 0.05) presence in mackerel muscle canned after freezing. A content increase (p < 0.05) was also observed for Na and Sn if prior frozen storage was also applied; on the contrary, Ca, Ba, Mn, Fe, Cd, S, and Se showed a content decrease (p < 0.05) as a result of such storage. Freezing, frozen storage, and canning led to lower values (p < 0.05) in canned fish for K, Mg, Pb, and P. Prior HPP led to relevant content decreases (p < 0.05) for K, Mg, Ca, Ba, Mn, Fe, Pb, and P contents in fish canned after the freezing step; HPP provoked additional decreases (p < 0.05) in Ca, Ba, and Mn levels in samples corresponding to 6-month frozen storage. On the contrary, prior HPP led to marked increases (p < 0.05) for Cd, S, and Se contents in all canned samples. Content changes are explained on the basis of modifications of other constituents and liquor losses from muscle.
Fighting Iron-Deficiency Anemia: Innovations in Food Fortificants and Biofortification Strategies
Authors: Liberal Ângela , Pinela José , Vívar-Quintana Ana Maria , Ferreira Isabel C. F. R. , Barros Lillian
Published: December 2020

Iron deficiency remains one of the main nutritional disorders worldwide and low iron intake and/or bioavailability are currently the major causes of anemia. To fight this public health problem, the scientific challenge is to find an iron form with sufficient bioavailability to increase its levels in humans through food fortification. In turn, biofortification appears as a comparatively advantageous and bearable strategy for the delivery of vitamins and other micronutrients for people without access to a healthy and diverse diet. This approach relies on plant breeding, transgenic techniques, or agronomic practices to obtain a final food product with a higher iron content. It is also known that certain food constituents are able to favor or inhibit iron absorption. The management of these compounds can thus successfully improve the absorption of dietary iron and, ultimately, contribute to fight this disorder present all over the world. This review describes the main causes/manifestations of iron-deficiency anemia, forms of disease prevention and treatment, and the importance of a balanced and preventive diet. A special focus was given to innovative food fortification and biofortification procedures used to improve the iron content in staple food crops.
LMI Criteria for Admissibility and Robust Stabilization of Singular Fractional-Order Systems Possessing Poly-Topic Uncertainties
Authors: Zhang Xuefeng , Dong Jia
Published: December 2020

The issue of robust admissibility and control for singular fractional-order systems (FOSs) with polytopic uncertainties is investigated in this paper. Firstly, a new method based on linear matrix inequalities (LMIs) is presented to solve the admissibility problems of uncertain linear systems. Then, a solid criterion of robust admissibility and a corresponding state feedback controller are derived, which overcome the conservatism of the existing results. Finally, for the sake of demonstrating the validity of proposed results, some relevant examples are provided.
Solventless Crosslinking of Chitosan, Xanthan, and Locust Bean Gum Networks Functionalized with β-Cyclodextrin
Authors: Petitjean Max , Aussant Florian , Vergara Ainara , Isasi José Ramón
Published: December 2020

The incorporation of cyclodextrins into polymeric crosslinked gels of hydrophilic nature can be useful for promoting the sorption of hydrophobic molecules and/or modulating the release of active principles. The covalent addition of these excipients to the matrix integrates their solubilizing effect that can contribute to increase the capacity of retention of hydrophobic substances. In this study, three diverse polysaccharides, chitosan, xanthan gum, and locust bean gum, were crosslinked with or without β-cyclodextrin, using citric acid in different ratios, to create hydrogel matrices. Through a green synthetic path, the efficient production of soluble and insoluble (hydrogel) networks functionalized with β-cyclodextrin was achieved by means of a solventless procedure. The characterization of their chemical composition, swelling in water, and their sorption and release behavior were also carried out in this work.
“We Were Queens.” Listening to Kānaka Maoli Perspectives on Historical and On-Going Losses in Hawai’i
Authors: Alvarez Antonia R.G. , Kanuha Val. Kalei , Anderson Maxine K.L. , Kapua Cathy , Bifulco Kris
Published: December 2020

This study examines a historical trauma theory-informed framework to remember Kānaka Maoli (Native Hawaiian) and lesbian, gay, bisexual, transgender, queer, and/or māhū (LGBTQM) experiences of colonization in Hawai`i. Kānaka Maoli people and LGBTQM Kānaka Maoli face health issues disproportionately when compared with racial and ethnic minorities in Hawai’i, and to the United States as a whole. Applying learnings from historical trauma theorists, health risks are examined as social and community-level responses to colonial oppressions. Through the crossover implementation of the Historical Loss Scale (HLS), this study makes connections between historical losses survived by Kānaka Maoli and mental health. Specifically, this manuscript presents unique ways that Kānaka Maoli describe and define historical losses, and place-based themes that emerged. These themes were: the militarization of land; the adoption of christianity by Kānaka Maoli ali`i; the overthrow of the sovereign Hawaiian monarch; and the importance of māhū and LGBTQ perspectives. Each of these themes will be presented in detail. Written by a queer, mestiza Pinay-American scholar, her mentor, a lesbian Kanaka Maoli scholar/activist, with contributions from Community Advisory Board members, there will also be discussion about ethics of research with and for Kānaka Maoli.
A Comprehensive Transcriptional Profiling of Pepper Responses to Root-Knot Nematode
Authors: Hu Weiming , Kingsbury Krista , Mishra Shova , DiGennaro Peter
Published: December 2020

Genetic resistance remains a key component in integrated pest management systems. The cosmopolitan root-knot nematode (RKN; Meloidogyne spp.) proves a significant management challenge as virulence and pathogenicity vary among and within species. RKN greatly reduces commercial bell pepper yield, and breeding programs continuously develop cultivars to emerging nematode threats. However, there is a lack of knowledge concerning the nature and forms of nematode resistance. Defining how resistant and susceptible pepper cultivars mount defenses against RKN attacks can help inform breeding programs. Here, we characterized the transcriptional responses of the highly related resistant (Charleston Belle) and susceptible (Keystone Resistance Giant) pepper cultivars throughout early nematode infection stages. Comprehensive transcriptomic sequencing of resistant and susceptible cultivar roots with or without Meloidogyneincognita infection over three-time points; covering early penetration (1-day), through feeding site maintenance (7-days post-inoculation), produced > 300 million high quality reads. Close examination of chromosome P9, on which nematode resistance hotspots are located, showed more differentially expressed genes were upregulated in resistant cultivar at day 1 when compared to the susceptible cultivar. Our comprehensive approach to transcriptomic profiling of pepper resistance revealed novel insights into how RKN causes disease and the plant responses mounted to counter nematode attack. This work broadens the definition of resistance from a single loci concept to a more complex array of interrelated pathways. Focus on these pathways in breeding programs may provide more sustainable and enduring forms of resistance.