bluuebunny/crossref_metadata_2025 huggingface dataset:
The Good, the Bad, and the Ugly: A Unified Approach to Behavioral Welfare Economics
Authors: Bernheim B. Douglas
Published: May 2016

This paper discusses the ways in which behavioral economics challenges the premises of conventional welfare economics. It proposes revised premises that survive those challenges and sets forth a welfare framework derived from that foundation. It argues that the proposed framework is practical, in the sense that it lends itself to applications, as well as unifying, in the sense that it subsumes other approaches and illuminates the relationships between them.
Use of Antibiotics Reserved for Resistant Gram-Negative Infections at Freestanding U.S. Children’s Hospitals from 2004 to 2014
Authors: Jelic Monika , Adler Amanda L. , Miles-Jay Arianna , Weissman Scott J. , Kronman Matthew P. , Zerr Danielle M.
Published: May 2016

We used the Pediatric Health Information System database to assess the use of antibiotics reserved for the treatment of resistant Gram-negative infections in children from 2004 to 2014. Overall, use of these agents increased in children from 2004 to 2007 and subsequently decreased.

Infect Control Hosp Epidemiol 2016:37:967–970
Silicon stable isotope fractionation between metal and silicate at high-pressure, high-temperature conditions as a tracer of planetary core formation
Authors: Kempl J. , Vroon P.Z. , van der Wagt B. , Zinngrebe E. , Frost D.J. , van Westrenen W.
Published: May 2016

The largest differentiation event in Earth and other terrestrial planets was the high-pressure, high-temperature process of metal core segregation from a silicate mantle. The abundant element silicon (Si) can be partially sequestered into the metallic core during metal–silicate differentiation, depending on pressure, temperature and planetary oxidation state. Knowledge of the Si content of a planet's core can constrain the conditions of core formation, but in the absence of direct samples from planetary cores, quantifying core Si content is challenging. One relatively new tool to study core formation in terrestrial planets is based on combining measurements of the Si stable isotopic composition of planetary crust and mantle samples with measurements of the Si stable isotope fractionation between metal and silicate at high-temperature and high-pressure conditions. In this study we present the results of a small set of high-pressure, high-temperature (HPT) experiments and combine these with a review of literature data to investigate how the Si isotope fractionation behaviour between metal and silicate varies as a function specifically of experimental run time and temperature. We show that although there is no debate about the sign of fractionation, absolute values for Si isotope fractionation between metal and silicate are difficult to constrain because the experimental database remains incomplete, and because Si isotopic measurements of metals in particular suffer from the absence of a true inter-laboratory comparison. We conclude that in order to derive accurate quantitative estimates of the Si content of the core of the Earth or other planets a wide range of additional experiments will be required.
Provenance signals in metaturbidites of the Paleoproterozoic greenstone belt of the Guiana Shield in Suriname
Authors: Naipal R. , Kroonenberg S.B.
Published: May 2016

The sedimentological, metamorphic, petrographic and geochemical characteristics of the Armina Formation, part of the Paleoproterozoic Greenstone Belt of Suriname in South America, are described, based on field, geochemical and petrographic evidence obtained through fieldwork along the Marowijne River and study of diamond drill cores from Rosebel Gold Mine (RGM). The metagreywackes show characteristic features of deposition by turbidity currents: coarse-grained, poorly sorted graded greywackes, covered by fine-grained, parallel-laminated phyllitic beds, often with convolute structures and climbing ripples. Their immature character and composition suggest deposition in an arc-trench environment. In the Marowijne River three different facies of metagreywackes are distinguished: (1) the greyish Bonnidoro Falls facies, characterised by common red millimetre-sized pseudomorphs after siderite in the finer beds, (2) the green Paroe Tabiki metagreywacke facies, with decimetre-sized calcsilicate nodules, both metamorphosed in the lower greenschist facies with chlorite as the main mafic mineral, and (3) the grey Armina Falls metagreywacke facies, geochemically similar to the Bonnidoro type but of higher metamorphic grade with biotite as the main mafic mineral. The metagreywackes from the Marowijne River show a predominance of quartz, plagioclase and lithic (tonalitic) clasts, suggesting exhumation of tonalite–trondhjemite–granodiorite plutons before deposition of the turbidites. There is a slight increase in maturity from (1) to (3), suggesting increasing weathering in the source areas. The metagreywackes of the RGM (JZone) have a predominantly metavolcanic origin, suggesting that they have a different provenance area than the Marowijne metagreywackes. Geochemically the spread in composition within each facies is larger than between the facies because of the wide range in grain sizes in each turbidite sequence. A large part of the rocks from the RGM, classified by previous authors as arenites, are geochemically and petrographically metagreywackes. Only a few RGM samples are real arenites, and plot as a separate cluster in geochemical factor score plots because of their low Fe and Na contents.
The role of rumen-protected choline in hepatic function and performance of transition dairy cows
Authors: Shahsavari Arash , D’Occhio Michael J. , Al Jassim Rafat
Published: May 2016

High-producing dairy cows enter a period of negative energy balance during the first weeks of lactation. Energy intake is usually sufficient to cover the increase in energy requirements for fetal growth during the period before calving, but meeting the demand for energy is often difficult during the early stages of lactation. A catabolic state predominates during the transition period, leading to the mobilisation of energy reserves (NEFA and amino acids) that are utilised mainly by the liver and muscle. Increased uptake of mobilised NEFA by the liver, combined with the limited capacity of hepatocytes to either oxidise fatty acids for energy or to incorporate esterified fatty acids into VLDL results in fatty liver syndrome and ketosis. This metabolic disturbance can affect the general health, and it causes economic losses. Different nutritional strategies have been used to restrict negative effects associated with the energy challenge in transition cows. The provision of choline in the form of rumen-protected choline (RPC) can potentially improve liver function by increasing VLDL exportation from the liver. RPC increases gene expression of microsomal TAG transfer protein and APOB100 that are required for VLDL synthesis and secretion. Studies with RPC have looked at gene expression, metabolic hormones, metabolite profiles, milk production and postpartum reproduction. A reduction in liver fat and enhanced milk production has been observed with RPC supplementation. However, the effects of RPC on health and reproduction are equivocal, which could reflect the lack of sufficient dose–response studies.
Phylogenetic relationships among reindeer lichens of North America
Authors: ATHUKORALA Sarangi N. P. , PINO-BODAS Raquel , STENROOS Soili , AHTI Teuvo , PIERCEY-NORMORE Michele D.
Published: May 2016

Cladoniais one of the largest lichen-forming ascomycete genera. It was formerly divided into ten sections, three of which,Crustaceae(Cladina),Tenues, andImpexae, are called the reindeer lichens. While previous studies have elucidated the relationships between species and sections, they often examined only one or a few specimens of each species in the analysis. This study examined the monophyly of selected members of sectionsCrustaceae,Tenues, andImpexaeand their relationships in the genusCladoniausing the internal transcribed spacer region of the nuclear ribosomal DNA (ITS rDNA) and the mitochondrial small subunit gene of the mitochondrial ribosomal DNA (mtSSU). The phylogenetic tree contained four clades, two representing species in sectionImpexae, one representing species that belong to sectionsCrustaceaeandTenues, and one clade withC. arbusculaand related species. Five of 22 species,C. pycnoclada,C. stellaris,C. evansii,C. ciliataandC. subtenuis, showed monophyly in the phylogenetic tree; some of these 5 species have been shown previously to be monophyletic. The thallus branching pattern was interpreted as an important heritable character using the mtSSU network. Three duplets of paraphyletic species were further examined using ITS rDNA haplotype networks and AMOVA analysis. The results for the species duplets showed some mixing of haplotypes but the AMOVA analysis provided support for species separation within the duplets. While the evidence supports distinct species, further study is needed to conclusively show separate species in these duplets.
Two new lecanoroid Caloplaca (Teloschistaceae) species from gneiss inselbergs in equatorial Brazil, with a key to tropical lecanoroid species of Caloplaca s. lat.
Authors: APTROOT André , CÁCERES Marcela Eugenia da Silva
Published: May 2016

Gneiss inselbergs in the Caatinga region of Ceará in equatorial Brazil are literally covered with a wide diversity of caloplacoid lichens. This includes two closely related new saxicolous species of Caloplaca, both with the aspect of a Lecanora of the subfusca-aggregate: C. lecanorocarpa has a grey areolate bullate thallus with atranorin on a black prothallus, grey thalline apothecium margin, sessile apothecia with glossy dark brown disc, and ascospores of 10–12×5·0–5·5 μm, with a 5·0–5·5 μm thick septum; and the similar, related C. lecapustulata which mainly differs by the rimose thallus with irregular mushroom-shaped pustules. The phylogenetic position of the two new species has been assessed to be within the Caloplacoideae. A key to all tropical lecanoroid Caloplaca species is provided.
Micarea soraliferasp. nov., a new sorediate species in theM. prasinagroup
Authors: GUZOW-KRZEMIŃSKA Beata , CZARNOTA Paweł , ŁUBEK Anna , KUKWA Martin
Published: May 2016

Micarea soraliferasp. nov., a new sorediate species belonging to theM. prasinagroup, is characterized by soralia developing directly from the endoxylic thallus or small external areoles, as well as the presence of micareic acid. Phylogenetic analyses of mtSSU rDNA sequences have shown that its closest relative isM. subviridescens. ITS rDNA sequence, a marker proposed as the universal barcoding region of fungi, was generated from the holotype.
A new species ofPorpidiafrom China
Authors: ZHAO Xiang-Xiang , ZHANG Lu-Lu , MIAO Cong-Cong , ZHAO Zun-Tian
Published: May 2016

Porpidia hyposticticais described as a new species; it has a white to grey-white thallus with yellow, oxidized patches near the margin, and contains hypostictic acid as the only major compound. A phylogenetic analysis is provided which is based on ITS sequences using ML and Bayesian analyses. The phylogeny supported the separation of the new species from other species. A key to all known ChinesePorpidiaspecies is provided.
Japan and the Asia-Pacific in the 1970s: From an economic to a ‘heart-to-heart’ relationship
Authors: CHIAPPONI CHIARA
Published: May 2016

This article deals with the relationships Japan has built in the Asia-Pacific region in the aftermath of decolonization. In post-war historic studies, the emphasis has been on issues such as paying reparations and providing economic assistance as Japan's means of rising to become a world power, at least from an economic point of view. The article explores, from a historical perspective, Japan's efforts with regard to development aid, but focuses on its transition to taking a more active role in Asia. This became more evident from the mid-1970s, when some crucial events related to the Cold War altered the the balance of power in the world. Hence, it investigates how Japan faced and took advantage of the situation in this area, and how it modified its approach to providing foreign assistance to Southeast Asia. Finally, it meditates upon the meaning of the Fukuda Doctrine as an enhancement of Tokyo's regional policy.
Krishna's Curse in the Age of Global Tourism: Hindu pilgrimage priests and their trade
Authors: AUKLAND KNUT
Published: May 2016

This article explores the strategies ofpandas (Hindu pilgrimage priests) in Vrindavan, relating changes in their trade (pandagiri) to tourism. These changes are the result of thepandas’ creative adjustments to shifting travel patterns that affect their market niche. Utilizing audio-recordings of thepandas’ guided tours, the article first portrays howpandas acquire ritual income from pilgrims by ‘inspiring’ donations of which they get a percentage. While commercial interests and economic conditions have always been crucial in shaping and perpetuating pilgrimage institutions and practices, global tourism has become an increasingly significant factor.Pandas all over India modify their services while the traditional exchange model (jajmanisystem) wanes. Changing travel patterns have made the guided tour a crucial component in the operation of Hindu pilgrimage. Vrindavanpandas have therefore turned into guides conducting religious sightseeing tours (darshan yatra). These tours are core to the new strategy for acquiring ritual income. To secure clients,pandas build connections with travel agencies and drivers and, in some cases, establish their own travel agencies that combine priestly and tourism services. Thepandas’ own understandings of their methods and contemporary travel trends further reflect the dynamic interplay between pilgrimage and tourism in India.
Are we working to save the species our children want to protect? Evaluating species attribute preferences among children
Authors: Frew Kristin , Peterson M. Nils , Stevenson Kathryn
Published: May 2016

As conservation resources decline and numbers of threatened species increase, prioritizing species for conservation is increasingly important, and prioritizing based on attributes may be the most efficient approach. Despite the importance of biodiversity as a legacy to future generations, children's preferences for species attributes have never been considered. We surveyed 3rd and 5th grade students, typically 8–10 years old, in North Carolina, USA, to determine how children prioritize conservation of species based on attributes. We asked the students to rank five species attributes, allocate money to species with each attribute, and choose between each species attribute and endemism in terms of their importance for conservation. Children prioritized species that are important in nature and those whose numbers are declining over species with other attributes, whereas research suggests that adults prioritize endemic species over most other types. Our results suggest children prioritize biodiversity conservation differently from adults, and in ways that may be more conducive to biodiversity conservation in cases where endemism is not directly related to species endangerment, and we suggest the perspectives of children be considered more fully within biodiversity conservation.
Crop raiding by wild mammals in Ethiopia: impacts on the livelihoods of smallholders in an agriculture–forest mosaic landscape
Authors: Ango Tola Gemechu , Börjeson Lowe , Senbeta Feyera
Published: May 2016

We assessed the impacts of crop raiding by wild mammals on the livelihoods of smallholding farmers in south-western Ethiopia. Data were generated through participatory field mapping, interviews and focus groups. The results indicated that wild mammals, mainly olive baboonsPapio anubisand bush pigsPotamochoerus larvatus, were raiding most crops cultivated in villages close to forests. In addition to the loss of crops, farmers incurred indirect costs in having to guard and cultivate plots far from their residences, sometimes at the expense of their children's schooling. Raiding also undermined farmers’ willingness to invest in modern agricultural technologies. Various coping strategies, including guarding crops and adapting existing local institutions, were insufficient to reduce raiding and its indirect impacts on household economies to tolerable levels, and were undermined by existing policies and government institutions. It is essential to recognize wild mammal pests as a critical ecosystem disservice to farmers, and to identify ways to mitigate their direct and indirect costs, to facilitate local agricultural development and livelihood security, and integrate wildlife conservation and local development more fully in agriculture–forest mosaic landscapes.
Adaptable Fuzzy Expert System for Ship Lock Control Support
Authors: Bačkalić Todor , Bugarski Vladimir , Kulić Filip , Kanović Željko
Published: May 2016

A ship lock zone represents a specific area on waterway, and control of the ship lockage process requires a comprehensive approach. This research is a practical application of a Mamdani-type fuzzy inference system and particle swarm optimisation to control this process. It presents an optimisation process that adapts control logic to the desired criteria. The initially proposed Fuzzy Expert System (FES) was developed using suggestions from lockmasters (ship lock operators) with extensive experience. Further optimisation of the membership function parameters of the input variables was performed to achieve better results in the local distribution of ship arrivals. The presented fuzzy logic-based expert system was designed as part of a Programmable Logic Controller (PLC) and Supervisory Control And Data Acquisition (SCADA) system to support decision making and control. The developed fuzzy algorithm is a rare application of artificial intelligence in navigable canals and significantly improves performance of the ship lockage process. This adaptable FES is designed to be used as a support in decision-making processes or for the direct control of ship lock operations.
International Criminal Law's Millennium of Forgotten History
Authors: Bohrer Ziv
Published: May 2016

At the close of World War II (WWII), Winston Churchill suggested summarily executing the remaining Nazi leadership. Franklin Delano Roosevelt disagreed, insisting on prosecuting them in an international military tribunal. This is considered the “birth” of International Criminal Law (ICL), following a consensus that “[t]he Nazi atrocities gave rise to the idea that some crimes are so grave as to concern the international community as a whole.” A few earlier instances of penal action against violators of the laws of war are acknowledged, but they are dismissed as unrelated to current ICL, because (presumably) these cases are sporadic domestic legal actions that lack a common doctrine.
A dynamic semisupervised feedforward neural network clustering
Authors: Asadi Roya , Kareem Sameem Abdul , Asadi Shokoofeh , Asadi Mitra
Published: May 2016

An efficient single-layer dynamic semisupervised feedforward neural network clustering method with one epoch training, data dimensionality reduction, and controlling noise data abilities is discussed to overcome the problems of high training time, low accuracy, and high memory complexity of clustering. Dynamically after the entrance of each new online input datum, the code book of nonrandom weights and other important information about online data as essentially important information are updated and stored in the memory. Consequently, the exclusive threshold of the data is calculated based on the essentially important information, and the data is clustered. Then, the network of clusters is updated. After learning, the model assigns a class label to the unlabeled data by considering a linear activation function and the exclusive threshold. Finally, the number of clusters and density of each cluster are updated. The accuracy of the proposed model is measured through the number of clusters, the quantity of correctly classified nodes, and F-measure. Briefly, in order to predict the survival time, the F-measure is 100% of the Iris, Musk2, Arcene, and Yeast data sets and 99.96% of the Spambase data set from the University of California at Irvine Machine Learning Repository; and the superior F-measure results in between 98.14% and 100% accuracies for the breast cancer data set from the University of Malaya Medical Center. We show that the proposed method is applicable in different areas, such as the prediction of the hydrate formation temperature with high accuracy.
IMMEDIATE POST-NATAL AIRWAY MANAGEMENT WHERE THERE ARE ANTENATAL CONCERNS
Authors: TAN NEIL , JONAS NICO
Published: May 2016

Management of the antenatal compromised airway is a situation that can at best be controlled and managed in a way that is safe for both mother and baby. As the 16th century author, Miguel de Cervantes wrote“Forewarned, forearmed; to be prepared is half the victory”, in a similar fashion we, as clinicians involved in the management of neonatal airways must recognise and prepare for all eventualities in order to produce the best outcomes for our patients. In this review, we discuss strategies in managing the compromised neonatal airway along with specific pathologies that may cause post-natal airway compromise. With each sub-group of pathologies, we suggest potential strategies that can be considered in their management.
Effect of temperature during embryonic development and first feeding of Trichogaster leeri larvae
Authors: Pereira Samuel Louzada , de Andrade Dalcio Ricardo , Radael Marcella Costa , Fosse Filho João Carlos , de Azevedo Rafael Vieira , Mattos Douglas da Cruz , Vidal Junior Manuel Vazquez
Published: May 2016

Temperature is an environmental factor that influences the development of fish, and when changed abruptly can lead to high mortality. Some species of fish are influenced by this factor, exhibiting a longer time for embryonic development and time to first feeding. This study aims to evaluate the effect of water temperature on embryonic and larval development up to first feeding, to describe the time in hours post fertilization (hpf) of the emergence of different structures and to determine the best hatching rate and survival of animals under different treatments. Five different egg incubation temperatures were used (24, 26, 28, 30 or 32°C, respectively). The eggs were observed at regular intervals of 30 min up to 24 h, every 2 h until 48 h and every 4 h until the display of first feeding in all treatments. Embryonic development was longer for eggs incubated at 24°C and the best results for hatching rate and survival of spawning efficiency were at 28°C. We recommend that incubation of Trichogaster leeri eggs is carried out at 28°C up to the first feeding of larvae.
Female fertility preservation strategies: cryopreservation and ovarian tissuein vitroculture, current state of the art and future perspectives
Authors: Filatov M.A. , Khramova Y.V. , Kiseleva M.V. , Malinova I.V. , Komarova E.V. , Semenova M.L.
Published: May 2016

In the present review, the main strategies of female fertility preservation are covered. Procedures of fertility preservation are necessary for women who suffer from diseases whose treatment requires the use of aggressive therapies, such as chemotherapy and radiotherapy. These kinds of therapy negatively influence the health of gametes and their progenitors. The most commonly used method of female fertility preservation is ovarian tissue cryopreservation, followed by the retransplantation of thawed tissue. Another approach to female fertility preservation that has been actively developed lately is the ovarian tissuein vitroculture. The principal methods, advantages and drawbacks of these two strategies are discussed in this article.
Social Cognition and Interaction Training (SCIT) for Adults with Psychotic Disorders: A Feasibility Study in Finland
Authors: Voutilainen Greta , Kouhia Tiina , Roberts David L. , Oksanen Jorma
Published: May 2016

Background: Social Cognition and Interaction Training (SCIT) is a psychosocial treatment designed to improve social functioning in schizophrenia by improving social cognition. Positive results have been reported from several studies, mainly from the USA, but more studies are needed to determine the feasibility of SCIT in different cultural contexts. Aims: The objective of this study was to evaluate the feasibility and acceptability of the Finnish translation of SCIT in Finland. Method: This was an uncontrolled, within-group study. Thirty-three patients with psychotic disorders participated in SCIT groups and also received the standard services provided at their respective care facilities. We measured participant attendance, attrition and responses on feedback surveys. Participants also completed measures of emotion perception, Theory of Mind (ToM), attributional bias and metacognitive overconfidence both before and after SCIT. Results: The attendance rate was high, attrition was low, and the patients expressed satisfaction with SCIT. Preliminary efficacy analyses showed a statistically significant pre to posttest improvement in emotion perception and ToM, but not attributional bias or overconfidence. Conclusions: SCIT is feasible and well accepted and may remediate social cognitive dysfunction in people with psychotic disorders in Finland.
Simultaneous Scanning Electron Microscope Imaging of Topographical and Chemical Contrast Using In-Lens, In-Column, and Everhart–Thornley Detector Systems
Authors: Zhang Xinming , Cen Xi , Ravichandran Rijuta , Hughes Lauren A. , van Benthem Klaus
Published: May 2016

The scanning electron microscope provides a platform for subnanometer resolution characterization of material morphology with excellent topographic and chemical contrast dependent on the used detectors. For imaging applications, the predominantly utilized signals are secondary electrons (SEs) and backscattered electrons (BSEs) that are emitted from the sample surface. Recent advances in detector technology beyond the traditional Everhart–Thornley geometry have enabled the simultaneous acquisition and discrimination of SE and BSE signals. This study demonstrates the imaging capabilities of a recently introduced new detector system that consists of the combination of two in-lens (I-L) detectors and one in-column (I-C) detector. Coupled with biasing the sample stage to reduce electron–specimen interaction volumes, this trinity of detector geometry allows simultaneous acquisition of signals to distinguish chemical contrast from topographical changes of the sample, including the identification of surface contamination. The I-C detector provides 4× improved topography, whereas the I-L detector closest to the sample offers excellent simultaneous chemical contrast imaging while not limiting the minimization of working distance to obtain optimal lateral resolution. Imaging capabilities and contrast mechanisms for all three detectors are discussed quantitatively in direct comparison to each other and the conventional Everhart–Thornley detector.
Increased Risk Of Early-Onset Neonatal Sepsis After Laser Surgery For Twin-to-Twin Transfusion Syndrome
Authors: van Kempen Liselotte E. M. , Zhao Depeng , Steggerda Sylke J. , Bekker Vincent , Middeldorp Johanna M. , Oepkes Dick , Lopriore Enrico
Published: May 2016

Objective: To investigate the occurrence of early-onset neonatal sepsis (EOS) in twin–twin transfusion syndrome (TTTS) managed with laser surgery.

Study design: We performed a prospective cohort study of all consecutive TTTS cases treated with laser surgery (TTTS group) delivered at the Leiden University Medical Center. We recorded the occurrence of EOS, defined as a positive blood culture ≤72 hours postpartum (proven sepsis) or administration of a full course of antibiotics due to risk factors or signs of sepsis, in the absence of a positive blood culture (suspected sepsis). Perinatal variables in the TTTS group were compared with uncomplicated monochorionic twins (no-TTTS group). A multivariate model was generated, examining the association between EOS and gestational age at birth, interval between laser surgery and birth, anterior placenta, laser period (first study period: 2002–2008; second study period: 2009–2015), and preterm premature rupture of membranes (PPROM).

Results: The rates of combined suspected and proven EOS in the TTTS group and no-TTTS group were 16% (68/416) and 10% (55/542), respectively (relative ratio [RR] 1.74, 95% confidence interval [CI] 1.19–2.55). Multivariate analysis showed that EOS in the TTTS group was independently associated with lower gestational age at birth (odds ratio [OR] 0.75, 95% CI 0.63–0.88), first study period (OR 2.25, 95% CI 1.08–4.67) and PPROM (OR 2.47, 95% CI 1.28–4.75).

Conclusion: The rate of EOS in the TTTS group is low, but increased compared to the no-TTTS group. EOS in TTTS is independently associated with premature delivery, earlier laser period, and PPROM.
Long-Term Neurodevelopmental Outcome in Survivors of Twin-to-Twin Transfusion Syndrome
Authors: van Klink Jeanine M. M. , Koopman Hendrik M. , Rijken Monique , Middeldorp Johanna M. , Oepkes Dick , Lopriore Enrico
Published: May 2016

Twin–twin transfusion syndrome (TTTS) is a severe complication of monochorionic (MC) twin pregnancies associated with high perinatal mortality and morbidity rates. Management in TTTS is a major challenge for obstetricians and neonatologists. Twins with TTTS are often born prematurely after an extremely distressing and highly hazardous fetal period. Follow-up studies report varying rates of cerebral palsy (CP) and long-term neurodevelopmental impairment (NDI). This review discusses the latest findings on the long-term outcome of TTTS survivors, possible risk factors for long-term impairment, and provides recommendations for future research.
Laminin regulates PDGFRβ+ cell stemness and muscle development
Authors: Yao Yao , Norris Erin H. , E. Mason Christopher , Strickland Sidney
Published: May 2016

Muscle-resident PDGFRβ+ cells, which include pericytes and PW1+ interstitial cells (PICs), play a dual role in muscular dystrophy. They can either undergo myogenesis to promote muscle regeneration or differentiate into adipocytes and other cells to compromise regeneration. How the differentiation and fate determination of PDGFRβ+ cells are regulated, however, remains unclear. Here, by utilizing a conditional knockout mouse line, we report that PDGFRβ+ cell-derived laminin inhibits their proliferation and adipogenesis, but is indispensable for their myogenesis. In addition, we show that laminin alone is able to partially reverse the muscle dystrophic phenotype in these mice at the molecular, structural and functional levels. Further RNAseq analysis reveals that laminin regulates PDGFRβ+ cell differentiation/fate determination via gpihbp1. These data support a critical role of laminin in the regulation of PDGFRβ+ cell stemness, identify an innovative target for future drug development and may provide an effective treatment for muscular dystrophy.
Wnt pathway activation by ADP-ribosylation
Authors: Yang Eungi , Tacchelly-Benites Ofelia , Wang Zhenghan , Randall Michael P. , Tian Ai , Benchabane Hassina , Freemantle Sarah , Pikielny Claudio , Tolwinski Nicholas S. , Lee Ethan , Ahmed Yashi
Published: May 2016

Wnt/β-catenin signalling directs fundamental processes during metazoan development and can be aberrantly activated in cancer. Wnt stimulation induces the recruitment of the scaffold protein Axin from an inhibitory destruction complex to a stimulatory signalosome. Here we analyse the early effects of Wnt on Axin and find that the ADP-ribose polymerase Tankyrase (Tnks)—known to target Axin for proteolysis—regulates Axin’s rapid transition following Wnt stimulation. We demonstrate that the pool of ADP-ribosylated Axin, which is degraded under basal conditions, increases immediately following Wnt stimulation in both Drosophila and human cells. ADP-ribosylation of Axin enhances its interaction with the Wnt co-receptor LRP6, an essential step in signalosome assembly. We suggest that in addition to controlling Axin levels, Tnks-dependent ADP-ribosylation promotes the reprogramming of Axin following Wnt stimulation; and propose that Tnks inhibition blocks Wnt signalling not only by increasing destruction complex activity, but also by impeding signalosome assembly.
Contributions of historical and contemporary geographic and environmental factors to phylogeographic structure in a Tertiary relict species, Emmenopterys henryi (Rubiaceae)
Authors: Zhang Yong-Hua , Wang Ian J. , Comes Hans Peter , Peng Hua , Qiu Ying-Xiong
Published: May 2016

Examining how historical and contemporary geographic and environmental factors contribute to genetic divergence at different evolutionary scales is a central yet largely unexplored question in ecology and evolution. Here, we examine this key question by investigating how environmental and geographic factors across different epochs have driven genetic divergence at deeper (phylogeographic) and shallower (landscape genetic) evolutionary scales in the Chinese Tertiary relict treeEmmenopterys henryi. We found that geography played a predominant role at all levels – phylogeographic clades are broadly geographically structured, the deepest levels of divergence are associated with major geological or pre-Quaternary climatic events and isolation by distance (IBD) primarily explained population genetic structure. However, environmental factors are clearly also important – climatic fluctuations since the Last Interglacial (LIG) have likely contributed to phylogeographic structure and the population genetic structure (in our AFLP dataset) was partly explained by isolation by environment (IBE), which may have resulted from natural selection in environments with divergent climates. Thus, historical and contemporary geography and historical and contemporary environments have all shaped patterns of genetic structure inE. henryi, and, in fact, changes in the landscape through time have also been critical factors.
Children’s altruistic behavior in context: The role of emotional responsiveness and culture
Authors: Rajhans Purva , Altvater-Mackensen Nicole , Vaish Amrisha , Grossmann Tobias
Published: May 2016

Altruistic behavior in humans is thought to have deep biological roots. Nonetheless, there is also evidence for considerable variation in altruistic behaviors among individuals and across cultures. Variability in altruistic behavior in adults has recently been related to individual differences in emotional responsiveness to fear in others. The current study examined the relation between emotional responsiveness (using eye-tracking) and altruistic behavior (using the Dictator Game) in 4 to 5-year-old children (N = 96) across cultures (India and Germany). The results revealed that increased altruistic behavior was associated with a greater responsiveness to fear faces (faster fixation), but not happy faces, in both cultures. This suggests that altruistic behavior is linked to our responsiveness to others in distress across cultures. Additionally, only among Indian children greater altruistic behavior was associated with greater sensitivity to context when responding to fearful faces. These findings further our understanding of the origins of altruism in humans by highlighting the importance of emotional processes and cultural context in the development of altruism.
Cross-tissue Analysis of Gene and Protein Expression in Normal and Cancer Tissues
Authors: Kosti Idit , Jain Nishant , Aran Dvir , Butte Atul J. , Sirota Marina
Published: May 2016

The central dogma of molecular biology describes the translation of genetic information from mRNA to protein, but does not specify the quantitation or timing of this process across the genome. We have analyzed protein and gene expression in a diverse set of human tissues. To study concordance and discordance of gene and protein expression, we integrated mass spectrometry data from the Human Proteome Map project and RNA-Seq measurements from the Genotype-Tissue Expression project. We analyzed 16,561 genes and the corresponding proteins in 14 tissue types across nearly 200 samples. A comprehensive tissue- and gene-specific analysis revealed that across the 14 tissues, correlation between mRNA and protein expression was positive and ranged from 0.36 to 0.5. We also identified 1,012 genes whose RNA and protein expression was correlated across all the tissues and examined genes and proteins that were concordantly and discordantly expressed for each tissue of interest. We extended our analysis to look for genes and proteins that were differentially correlated in cancer compared to normal tissues, showing higher levels of correlation in normal tissues. Finally, we explored the implications of these findings in the context of biomarker and drug target discovery.
Diffraction-limited ultrabroadband terahertz spectroscopy
Authors: Baillergeau M. , Maussang K. , Nirrengarten T. , Palomo J. , Li L. H. , Linfield E. H. , Davies A. G. , Dhillon S. , Tignon J. , Mangeney J.
Published: May 2016

Diffraction is the ultimate limit at which details of objects can be resolved in conventional optical spectroscopy and imaging systems. In the THz spectral range, spectroscopy systems increasingly rely on ultra-broadband radiation (extending over more 5 octaves) making a great challenge to reach resolution limited by diffraction. Here, we propose an original easy-to-implement wavefront manipulation concept to achieve ultrabroadband THz spectroscopy system with diffraction-limited resolution. Applying this concept to a large-area photoconductive emitter, we demonstrate diffraction-limited ultra-broadband spectroscopy system up to 14.5 THz with a dynamic range of 103. The strong focusing of ultrabroadband THz radiation provided by our approach is essential for investigating single micrometer-scale objects such as graphene flakes or living cells and besides for achieving intense ultra-broadband THz electric fields.
Contrasting global genetic patterns in two biologically similar, widespread and invasive Ciona species (Tunicata, Ascidiacea)
Authors: Bouchemousse Sarah , Bishop John D. D. , Viard Frédérique
Published: May 2016

Human-mediated dispersal interplays with natural processes and complicates understanding of the biogeographical history of species. This is exemplified by two invasive tunicates, Ciona robusta (formerly Ciona intestinalis type A) and C. intestinalis (formerly Ciona intestinalis type B), globally distributed and sympatric in Europe. By gathering new mitochondrial sequences that were merged with published datasets, we analysed genetic patterns in different regions, with a focus on 1) their sympatric range and 2) allopatric populations in N and S America and southern Europe. In the sympatric range, the two species display contrasting genetic diversity patterns, with low polymorphism in C. robusta supporting the prevalent view of its recent introduction. In the E Pacific, several genetic traits support the non-native status of C. robusta. However, in the NE Pacific, this appraisal requires a complex scenario of introduction and should be further examined supported by extensive sampling efforts in the NW Pacific (putative native range). For C. intestinalis, Bayesian analysis suggested a natural amphi-North Atlantic distribution, casting doubt on its non-native status in the NW Atlantic. This study shows that both natural and human-mediated dispersal have influenced genetic patterns at broad scales; this interaction lessens our ability to confidently ascertain native vs. non-native status of populations, particularly of those species that are globally distributed.
New recombinant cyclohexylamine oxidase variants for deracemization of secondary amines by orthogonally assaying designed mutants with structurally diverse substrates
Authors: Li Guangyue , Yao Peiyuan , Cong Peiqian , Ren Jie , Wang Lei , Feng Jinhui , Lau Peter C.K. , Wu Qiaqing , Zhu Dunming
Published: May 2016

To further expand the substrate range of the cyclohexylamine oxidase (CHAO) fromBrevibacterium oxydans, a library of diverse mutants was created and assayed toward a group of structurally diverse substrates. Among them, mutants T198A and M226A exhibited enhanced activity relative to wt CHAO for most (S)-enantiomers of primary amines and some secondary amines. While mutants T198I, L199I, L199F, M226I and M226T were more active than wt CHAO toward the primary amines, mutants T198F, L199T, Y321A, Y321T, Y321I and Y321F enhanced the enzyme activity toward the secondary amines. In particular, mutant Y321I displayed an enhanced catalytic efficiency toward 1-(4-methoxybenzyl)-1, 2, 3, 4, 5, 6, 7, 8-octahydroisoquinoline (13). Whereas a double mutant, Y321I/M226T, acted on (S)-N-(prop-2-yn-1-yl)-2, 3-dihydro-1H-inden-1-amine [(S)-8]. Since (R)-8is an irreversible inhibitor of monoamine oxidase and (S)-13is an intermediate of dextromethorphan, a cough suppressant drug, deracemizations of8and13were carried out with crude enzyme extracts of the respective mutants. This resulted in 51% and 78% isolated yields of (R)-8and (S)-13, respectively, each with high enantiomeric excess (93% and 99% ee). The results demonstrated the application potential of the evolved CHAO mutants in drug synthesis requiring chiral secondary amines.
Stationary bubble formation and cavity collapse in wedge-shaped hoppers
Authors: Yagisawa Yui , Then Hui Zee , Okumura Ko
Published: May 2016

The hourglass is one of the apparatuses familiar to everyone, but reveals intriguing behaviors peculiar to granular materials, and many issues are remained to be explored. In this study, we examined the dynamics of falling sand in a special form of hourglass, i.e., a wedge-shaped hopper, when a suspended granular layer is stabilized to a certain degree. As a result, we found remarkably different dynamic regimes of bubbling and cavity. In the bubbling regime, bubbles of nearly equal size are created in the sand at a regular time interval. In the cavity regime, a cavity grows as sand beads fall before a sudden collapse of the cavity. Bubbling found here is quite visible to a level never discussed in the physics literature and the cavity regime is a novel phase, which is neither continuous, intermittent nor completely blocked phase. We elucidate the physical conditions necessary for the bubbling and cavity regimes and develop simple theories for the regimes to successfully explain the observed phenomena by considering the stability of a suspended granular layer and clogging of granular flow at the outlet of the hopper. The bubbling and cavity regimes could be useful for mixing a fluid with granular materials.
Charge collection kinetics on ferroelectric polymer surface using charge gradient microscopy
Authors: Choi Yoon-Young , Tong Sheng , Ducharme Stephen , Roelofs Andreas , Hong Seungbum
Published: May 2016

A charge gradient microscopy (CGM) probe was used to collect surface screening charges on poly(vinylidene fluoride-trifluoroethylene) [P(VDF-TrFE)] thin films. These charges are naturally formed on unscreened ferroelectric domains in ambient condition. The CGM data were used to map the local electric current originating from the collected surface charges on the poled ferroelectric domains in the P(VDF-TrFE) thin films. Both the direction and amount of the collected current were controlled by changing the polarity and area of the poled domains. The endurance of charge collection by rubbing the CGM tip on the polymer film was limited to 20 scan cycles, after which the current reduced to almost zero. This degradation was attributed to the increase of the chemical bonding strength between the external screening charges and the polarization charges. Once this degradation mechanism is mitigated, the CGM technique can be applied to efficient energy harvesting devices using polymer ferroelectrics.
pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCD- and CID-MS/MS and MS3
Authors: Zeng Wen-Feng , Liu Ming-Qi , Zhang Yang , Wu Jian-Qiang , Fang Pan , Peng Chao , Nie Aiying , Yan Guoquan , Cao Weiqian , Liu Chao , Chi Hao , Sun Rui-Xiang , Wong Catherine C. L. , He Si-Min , Yang Pengyuan
Published: May 2016

Confident characterization of the microheterogeneity of protein glycosylation through identification of intact glycopeptides remains one of the toughest analytical challenges for glycoproteomics. Recently proposed mass spectrometry (MS)-based methods still have some defects such as lack of the false discovery rate (FDR) analysis for the glycan identification and lack of sufficient fragmentation information for the peptide identification. Here we proposed pGlyco, a novel pipeline for the identification of intact glycopeptides by using complementary MS techniques: 1) HCD-MS/MS followed by product-dependent CID-MS/MS was used to provide complementary fragments to identify the glycans and a novel target-decoy method was developed to estimate the false discovery rate of the glycan identification; 2) data-dependent acquisition of MS3 for some most intense peaks of HCD-MS/MS was used to provide fragments to identify the peptide backbones. By integrating HCD-MS/MS, CID-MS/MS and MS3, intact glycopeptides could be confidently identified. With pGlyco, a standard glycoprotein mixture was analyzed in the Orbitrap Fusion and 309 non-redundant intact glycopeptides were identified with detailed spectral information of both glycans and peptides.
Identification and manipulation of the pleuromutilin gene cluster from Clitopilus passeckerianus for increased rapid antibiotic production
Authors: Bailey Andy M. , Alberti Fabrizio , Kilaru Sreedhar , Collins Catherine M. , de Mattos-Shipley Kate , Hartley Amanda J. , Hayes Patrick , Griffin Alison , Lazarus Colin M. , Cox Russell J. , Willis Christine L. , O’Dwyer Karen , Spence David W. , Foster Gary D.
Published: May 2016

Semi-synthetic derivatives of the tricyclic diterpene antibiotic pleuromutilin from the basidiomycete Clitopilus passeckerianus are important in combatting bacterial infections in human and veterinary medicine. These compounds belong to the only new class of antibiotics for human applications, with novel mode of action and lack of cross-resistance, representing a class with great potential. Basidiomycete fungi, being dikaryotic, are not generally amenable to strain improvement. We report identification of the seven-gene pleuromutilin gene cluster and verify that using various targeted approaches aimed at increasing antibiotic production in C. passeckerianus, no improvement in yield was achieved. The seven-gene pleuromutilin cluster was reconstructed within Aspergillus oryzae giving production of pleuromutilin in an ascomycete, with a significant increase (2106%) in production. This is the first gene cluster from a basidiomycete to be successfully expressed in an ascomycete, and paves the way for the exploitation of a metabolically rich but traditionally overlooked group of fungi.
The arachidonic acid metabolite 11β-ProstaglandinF2α controls intestinal epithelial healing: deficiency in patients with Crohn’s disease
Authors: Coquenlorge Sabrina , Van Landeghem Laurianne , Jaulin Julie , Cenac Nicolas , Vergnolle Nathalie , Duchalais Emilie , Neunlist Michel , Rolli-Derkinderen Malvyne
Published: May 2016

In healthy gut enteric glial cells (EGC) are essential to intestinal epithelial barrier (IEB) functions. In Crohn’s Disease (CD), both EGC phenotype and IEB functions are altered, but putative involvement of EGC in CD pathogenesis remains unknown and study of human EGC are lacking. EGC isolated from CD and control patients showed similar expression of glial markers and EGC-derived soluble factors (IL6, TGF-β, proEGF, GSH) but CD EGC failed to increase IEB resistance and healing. Lipid profiling showed that CD EGC produced decreased amounts of 15-HETE, 18-HEPE, 15dPGJ2 and 11βPGF2α as compared to healthy EGC. They also had reduced expression of the L-PGDS and AKR1C3 enzymes. Produced by healthy EGC, the 11βPGF2 activated PPARγ receptor of intestinal epithelial cells to induce cell spreading and IEB wound repair. In addition to this novel healing mechanism our data show that CD EGC presented impaired ability to promote IEB functions through defect in L-PGDS-AKR1C3-11βPGF2α dependent pathway.