bluuebunny/crossref_metadata_2025 huggingface dataset:
Are Methylation Patterns in the KALRN Gene Associated with Cognitive and Depressive Symptoms? Findings from the Moli-sani Cohort
Authors: Quiccione Miriam Shasa , Tirozzi Alfonsina , Cassioli Giulia , Morelli Martina , Costanzo Simona , Pepe Antonietta , Bracone Francesca , Magnacca Sara , Cerletti Chiara , Licastro Danilo , Di Castelnuovo Augusto , Donati Maria Benedetta , de Gaetano Giovanni , Iacoviello Licia , Gialluisi Alessandro
Published: September 2024

The KALRN gene (encoding kalirin) has been implicated in several neuropsychiatric and neurodegenerative disorders. However, genetic evidence supporting this implication is limited and targeted epigenetic analyses are lacking. Here, we tested associations between epigenetic variation in KALRN and interindividual variation in depressive symptoms (PHQ9) and cognitive (MoCA) performance, in an Italian population cohort (N = 2409; mean (SD) age: 67 (9) years; 55% women). First, we analyzed the candidate region chr3:124584826–124584886 (hg38), within the KALRN promoter, through pyrosequencing of 1385 samples. Then, we widened the investigated region by analyzing 137 CpGs annotated to the whole gene, rescued from epigenome-wide (Illumina EPIC) data from 1024 independent samples from the same cohort. These were tested through stepwise regression models adjusted for age, sex, circulating leukocytes fractions, education, prevalent health conditions and lifestyles. We observed no statistically significant associations with methylation levels in the three CpGs tested through pyrosequencing, or in the gene-wide association analysis with MoCA score. However, we observed a statistically significant association between PHQ9 and cg13549966 (chr3:124106738; β (Standard Error) = 0.28 (0.08), Bonferroni-corrected p = 0.025), located close to the transcription start site of the gene. This association was driven by a polychoric factor tagging somatic depressive symptoms (β (SE) = 0.127 (0.064), p = 0.048). This evidence underscores the importance of studying epigenetic variation within the KALRN gene and the role that it may play in brain diseases, particularly in atypical depression, which is often characterized by somatic symptoms.
Explainable Ensemble Learning Approaches for Predicting the Compression Index of Clays
Authors: Ge Qi , Xia Yijie , Shu Junwei , Li Jin , Sun Hongyue
Published: September 2024

Accurate prediction of the compression index (cc) is essential for geotechnical infrastructure design, especially in clay-rich coastal regions. Traditional methods for determining cc are often time-consuming and inconsistent due to regional variability. This study presents an explainable ensemble learning framework for predicting the cc of clays. Using a comprehensive dataset of 1080 global samples, four key geotechnical input variables—liquid limit (LL), plasticity index (PI), initial void ratio (e0), and natural water content w—were leveraged for accurate cc prediction. Missing data were addressed with K-Nearest Neighbors (KNN) imputation, effectively filling data gaps while preserving the dataset’s distribution characteristics. Ensemble learning techniques, including Random Forest (RF), Gradient Boosting Decision Trees (GBDT), Extreme Gradient Boosting (XGBoost), and a Stacking model, were applied. Among these, the Stacking model demonstrated the highest predictive performance with a Root Mean Squared Error (RMSE) of 0.061, a Mean Absolute Error (MAE) of 0.043, and a Coefficient of Determination (R2) value of 0.848 on the test set. Model interpretability was ensured through SHapley Additive exPlanations (SHAP), with e0 identified as the most influential predictor. The proposed framework significantly improves both prediction accuracy and interpretability, offering a valuable tool to enhance geotechnical design efficiency in coastal and clay-rich environments.
Artificial Cultivation of Aquatic Plants Promotes Nitrogen Transformation and the Abundance of Key Functional Genes in Agricultural Drainage Ditch Sediments in the Yellow River Irrigation Area in China
Authors: Hong Yu , He Ziqi , Liu Ruliang , Xiang Wenhua , Lei Pifeng , Fang Xi
Published: September 2024

Excess nitrogen in agricultural drainage poses a serious threat to the water quality safety of the Yellow River basin. Utilizing aquatic plants to modify the rhizosphere microbial community structure and facilitate nitrogen transformation is a crucial strategy for mitigating regional water eutrophication. We here compare key processes of nitrogen transformation occurring in the rhizosphere of sediments of a ditch artificially planted with a mix of species (Phragmites australis, Typha orientalis, Nymphaea tetragon) with the rhizosphere of a ditch occupied by naturally occurring aquatic vegetation, dominated either by P. australis or T. orientalis. Our results revealed a species effect, with an increased denitrification rate (DR) and dissimilatory nitrate reduction to ammonium rate (DNRAR) in the cultivated ditch for P. australis, compared to the naturally occurring T. orientalis vegetation. The nitrogen fixation rate (NFR) increased in the artificial setting with T. orientalis in comparison to natural P. australis vegetation. The richness of the bacterial community and the relative abundances of Bacteroidota, Firmicutes, and Geobacter were significantly greater in the rhizosphere of the artificially cultivated ditch due a greater availability in nitrogen and organic carbon. In the artificially cultivated ditch, the dominant functional genes affecting DRNARs in the rhizosphere sediments of P. australis were nrfC and nrfA, whereas DRs were driven mainly by norB and napA, which were influenced by the nitrogen and carbon levels. The dominant functional genes affecting NFRs in the rhizosphere sediments of T. orientalis were nifD, nifK, and nifH. Our results provide a scientific basis for the use of aquatic plants for mitigating excess nitrogen levels in agricultural drainage.
Network Pharmacology, Molecular Dynamics and In Vitro Assessments of Indigenous Herbal Formulations for Alzheimer’s Therapy
Authors: Ojo Oluwafemi Adeleke , Ajayi-Odoko Omolola Adenike , Gyebi Gideon Ampoma , Ayokunle Damilare IyinKristi , Ogunlakin Akingbolabo Daniel , Ezenabor Emmanuel Henry , Olanrewaju Adesoji Alani , Agbeye Oluwatobi Deborah , Ogunwale Emmanuel Tope , Rotimi Damilare Emmanuel , Fouad Dalia , Batiha Gaber El-Saber , Adeyemi Oluyomi Stephen
Published: September 2024

Alzheimer’s disease (AD) is an age-associated neurodegenerative condition marked by amyloid plaques, synaptic dysfunction, and neuronal loss. Besides conventional medical care, herbal therapies, both raw and refined, have attracted researchers for their potential therapeutic effects. As a proof-of-concept, our study combined HPLC-DAD analysis of bioactive constituents, network pharmacology, molecular dynamics (MD), molecular docking, post-MD analysis, and experimental verification to investigate the mechanisms of crude drug formulations as a therapeutic strategy for AD. We identified nine bioactive compounds targeting 188 proteins and 1171 AD-associated genes. Using a Venn diagram, we found 47 overlapping targets, forming “herb-compound-target (HCT)” interaction networks and a protein‒protein interaction (PPI) network. Simulations analyzed binding interactions among the three core targets and their compounds. MD assessed the stability of the best-ranked poses and beneficial compounds for each protein. Among the top 22 hub genes, AChE, BChE, and MAO, ranked 10, 14, and 34, respectively, were selected for further analysis. Two tetraherbal formulations, Form A and Form B, showed notable activity against AChE. Form A exhibited significant (p < 0.0001) inhibitory activity (IC50 = 114.842 ± 2.084 µg/mL) compared to Form B (IC50 = 142.829 ± 4.258 µg/mL), though weaker than galantamine (IC50 = 27.950 ± 0.122 µg/mL). Form B had significant inhibitory effects on BChE (IC50 = 655.860 ± 32.812 µg/mL) compared to Form A (IC50 = 679.718 ± 20.656 µg/mL), but lower than galantamine (IC50 = 23.126 ± 0.683 µg/mL). Both forms protected against Fe2+-mediated brain injury by inhibiting MAO. Docking identified quercetin (−10.2 kcal/mol) and myricetin (−10.1 kcal/mol) for AChE; rutin (−10.6 kcal/mol) and quercetin (−9.7 kcal/mol) for BChE; and kaempferol (−9.1 kcal/mol) and quercetin (−8.9 kcal/mol) for MAO. These compounds were thermodynamically stable based on MD analysis. Collectively, the results offer a scientific rationale for the use of these specifically selected medicinal herbs as AD medications.
Migraine and Pregnancy
Authors: Phillips Katherine , Koonalintip Prut , Wakerley Benjamin R.
Published: September 2024

Migraine affects almost one in five women of reproductive age. Therefore, understanding its impact on pregnancy outcomes and how to manage migraine safely in pregnancy are of particular importance. This review will summarise the clinical course of migraine during pregnancy, the management of women presenting with headaches during pregnancy, the management of migraine during pregnancy and summarise what is known about how migraine and migraine medications impact pregnancy outcomes.
Multi-Parametric Investigations on White Etching Crack Formation in Deep Grove Ball Bearings
Authors: Zuercher Manuel , Schlücker Eberhard , Spaeth Christian , Holweger Walter
Published: September 2024

Research on White Etching Cracks (WEC) in multiple bearing applications has identified various drivers that cause them. Lubricants and electricity combined with contact mechanics have been proven to catalyze WEC significantly. However, none of these factors solely cause WEC on its own; instead, combinations of factors discretize whether WEC appears or not. Hence, the WEC phenomenon appears to be multidimensional, making WEC still unpredictable. The current paper is about a systematic study using a Deep Grove Ball Bearing test rig to investigate how lubricant chemicals, combined with electricity and variations in oil flow and pressure, lead to WEC formation. It becomes evident that even under critical conditions for WEC formation, increasing oil flow and decreasing contact pressure can prevent WEC.
The Effect of the Calcination Time on the Microstructure and Properties of MnZn Ferrite Powders
Authors: Xu Zhanyuan , Zhao Wei , Bai Lichun , Fan Jinglian
Published: September 2024

MnZn ferrite powders were prepared based on the novel nano in situ composite method and through chemical sol-spray drying–calcination technology. The precursor powders were calcined at 1060 °C at different calcination times (1–9 h) to research the influences of the calcination time on MnZn ferrite powders. The research results revealed that all samples had similar morphologies composed of fine particles. The pure MnZn ferrite spinel phase can only be obtained when the calcination time does not exceed 3 h. Otherwise, some α-Fe2O3 or γ-Fe2O3 impurities will appear. The particle size descended with an increasing calcination time and then ascended. After 3 h of preservation, the smallest particle size was obtained, and it exhibited a unimodal distribution. The saturation magnetization (Ms) increased at first and decreased later with an increasing calcination time, and the optimal value (53.4 emu/g) was reached after holding for 3 h. In view of this work, the optimal calcination time is 3 h.
Asymptotics for Finite-Time Ruin Probabilities of a Dependent Bidimensional Risk Model with Stochastic Return and Subexponential Claims
Authors: Shen Xiaowen , Wang Kaiyong , Yang Yang
Published: September 2024

The paper considers a bidimensional continuous-time risk model with subexponential claims and Brownian perturbations, in which the price processes of the investment portfolio of the two lines of business are two geometric Lévy processes and the two lines of business share a common claim-number process, which is a renewal counting process. The paper mainly considers the claims of each line of business having a dependence structure. When the claims have subexponential distributions, the asymptotics of the finite-time ruin probabilities ψand(x1,x2;T) and ψsim(x1,x2;T) have been obtained. When the distributions of claims belong to the intersection of long-tailed and dominatedly varying-tailed distribution classes, the asymptotics of the finite-time ruin probability ψor(x1,x2;T) is given.
A Zassenhaus Lemma for Digroups
Authors: Biyogmam Guy Roger
Published: September 2024

In this paper, we construct a quotient structure on digroups. This construction yields a new functor from the category of digroups to the category of groups. We obtain a modular property for digroups and use it to prove an analogue of the Zassenhaus lemma in this framework.
Intelligent Reflecting Surface-Assisted Wireless Communication Using RNNs: Comprehensive Insights
Authors: Tabassum Rana , Sejan Mohammad Abrar Shakil , Rahman Md Habibur , Aziz Md Abdul , Song Hyoung-Kyu
Published: September 2024

By adjusting the propagation environment using reconfigurable reflecting elements, intelligent reflecting surfaces (IRSs) have become potential techniques used to improve the efficiency of wireless communication networks. In IRS-assisted communication systems, accurate channel estimation is crucial for optimizing signal transmission and achieving high spectral efficiency. As mobile data traffic continues to surge and the demand for high-capacity and low-latency wireless connectivity grows, IRSs are becoming pivotal technologies in the development of next-generation communication networks. IRSs offer the potential to revolutionize wireless propagation environments, improving network capacity and coverage, particularly in high-frequency wave scenarios where traditional signals encounter obstacles. Amidst this evolving landscape, machine learning (ML) emerges as a powerful tool to harness the full potential of IRS-assisted communication systems, particularly given the escalating computational complexity associated with deploying and operating IRSs in dynamic environments. This paper presents an overview of preliminary results for IRS-assisted communication using recurrent neural networks (RNNs). We first implement single- and double-layer LSTM, BiLSTM, and GRU techniques for an IRS-based communication system. In the next phase, we explore a hybrid approach, combining different RNN techniques, including LSTM-BiLSTM, LSTM-GRU, and BiLSTM-GRU, as well as their reverse configurations. These RNN algorithms were evaluated with respect to bit error rate (BER) and symbol error rate (SER) for IRS-enhanced communication. According to the experimental results, the BiLSTM double-layer model and the BiLSTM-GRU combination demonstrated the highest BER and SER accuracy compared to other approaches.
Solving the Robust Shortest Path Problem with Multimodal Transportation
Authors: Guo Jinzuo , Liu Tianyu , Song Guopeng , Guo Bo
Published: September 2024

This paper explores the challenges of finding robust shortest paths in multimodal transportation networks. With the increasing complexity and uncertainties in modern transportation systems, developing efficient and reliable routing strategies that can adapt to various disruptions and modal changes is essential. By incorporating practical constraints in parameter uncertainty, this paper establishes a robust shortest path mixed-integer programming model based on a multimodal transportation network under transportation time uncertainty. To solve robust shortest path problems with multimodal transportation, we propose a modified Dijkstra algorithm that integrates parameter uncertainty with multimodal transportation. The effectiveness of the proposed multimodal transportation shortest path algorithm is verified using empirical experiments on test sets of different scales and a comparison of the runtime using a commercial solver. The experimental results on the multimodal transportation networks demonstrate the effectiveness of our approach in providing robust and efficient routing solutions. The results demonstrate that the proposed method can generate optimal solutions to the robust shortest path problem in multimodal transportation under time uncertainty and has practical significance.
Digital Twin Framework for Aircraft Lifecycle Management Based on Data-Driven Models
Authors: Kabashkin Igor
Published: September 2024

This paper presents a comprehensive framework for implementing digital twins in aircraft lifecycle management, with a focus on using data-driven models to enhance decision-making and operational efficiency. The proposed framework integrates cutting-edge technologies such as IoT sensors, big data analytics, machine learning, 6G communication, and cloud computing to create a robust digital twin ecosystem. This paper explores the key components of the framework, including lifecycle phases, new technologies, and models for digital twins. It discusses the challenges of creating accurate digital twins during aircraft operation and maintenance and proposes solutions using emerging technologies. The framework incorporates physics-based, data-driven, and hybrid models to simulate and predict aircraft behavior. Supporting components like data management, federated learning, and analytics tools enable seamless integration and operation. This paper also examines decision-making models, a knowledge-driven approach, limitations of current implementations, and future research directions. This holistic framework aims to transform fragmented aircraft data into comprehensive, real-time digital representations that can enhance safety, efficiency, and sustainability throughout the aircraft lifecycle.
Semisynthesis, Structure Elucidation and Anti-Mycobacterium marinum Activity of a Series of Marine-Derived 14-Membered Resorcylic Acid Lactones with Interesting Ketal Groups
Authors: Yin Jun-Na , Wang Cui-Fang , Zhang Xiu-Li , Cheng Ya-Jie , Wu Yan-Wei , Zhang Qun , Shao Chang-Lun , Wei Mei-Yan , Gu Yu-Cheng
Published: September 2024

The incidence of Mycobacterium marinum infection is on the rise; however, the existing drug treatment cycle is lengthy and often requires multi-drug combination. Therefore, there is a need to develop new and effective anti-M. marinum drugs. Cochliomycin A, a 14-membered resorcylic acid lactone with an acetonide group at C-5′ and C-6′, exhibits a wide range of antimicrobial, antimalarial, and antifouling activities. To further explore the effect of this structural change at C-5′ and C-6′ on this compound’s activity, we synthesized a series of compounds with a structure similar to that of cochliomycin A, bearing ketal groups at C-5′ and C-6′. The R/S configuration of the diastereoisomer at C-13′ was further determined through an NOE correlation analysis of CH3 or CH2 at the derivative C-13′ position and the H-5′ and H-6′ by means of a 1D NOE experiment. Further comparative 1H NMR analysis of diastereoisomers showed the difference in the chemical shift (δ) value of the diastereoisomers. The synthetic compounds were screened for their anti-microbial activities in vitro. Compounds 15–24 and 28–35 demonstrated promising activity against M. marinum, with MIC90 values ranging from 70 to 90 μM, closely approaching the MIC90 of isoniazid. The preliminary structure–activity relationships showed that the ketal groups with aromatic rings at C-5′ and C-6′ could enhance the inhibition of M. marinum. Further study demonstrated that compounds 23, 24, 29, and 30 had significant inhibitory effects on M. marinum and addictive effects with isoniazid and rifampicin. Its effective properties make it an important clue for future drug development toward combatting M. marinum resistance.
Natural Products from Marine-Derived Fungi with Anti-Inflammatory Activity
Authors: Qiu Yikang , Chen Shiji , Yu Miao , Shi Jueying , Liu Jiayu , Li Xiaoyang , Chen Jiaxing , Sun Xueping , Huang Guolei , Zheng Caijuan
Published: September 2024

Inflammation is considered as one of the most primary protective innate immunity responses, closely related to the body’s defense mechanism for responding to chemical, biological infections, or physical injuries. Furthermore, prolonged inflammation is undesirable, playing an important role in the development of various diseases, such as heart disease, diabetes, Alzheimer’s disease, atherosclerosis, rheumatoid arthritis, and even certain cancers. Marine-derived fungi represent promising sources of structurally novel bioactive natural products, and have been a focus of research for the development of anti-inflammatory drugs. This review covers secondary metabolites with anti-inflammatory activities from marine-derived fungi, over the period spanning August 2018 to July 2024. A total of 285 anti-inflammatory metabolites, including 156 novel compounds and 11 with novel skeleton structures, are described. Their structures are categorized into five categories: terpenoids, polyketides, nitrogen-containing compounds, steroids, and other classes. The biological targets, as well as the in vitro and in vivo screening models, were surveyed and statistically summarized. This paper aims to offer valuable insights to researchers in the exploration of natural products and the discovery of anti-inflammatory drugs.
Purification of Liquid Fraction of Digestates from Different Origins—Comparison of Polymeric and Ceramic Ultrafiltration Membranes Used for This Purpose
Authors: Urbanowska Agnieszka
Published: September 2024

Circular economy, clean technologies, and renewable energy are key to climate protection and modern environmental technology. Recovering water and valuable minerals from the liquid fraction of digestate is in line with this strategy. Digestate, a byproduct of anaerobic methane fermentation in biogas plants, is a potential source of water, minerals for fertilizers, and energy rather than waste. This study examined digestate from municipal and agricultural biogas plants and highlights the need for research on both due to their differences. The use of membrane techniques for water recovery from liquid digestate offers an innovative alternative to conventional methods. This study used standalone membrane filtration and an integrated system to produce water suitable for agricultural use. Ceramic membranes with cut-offs of 1, 5, 15, and 50 kDa and polymeric membranes of polyethersulfone and regenerated cellulose with cut-offs of 10 and 30 kDa were tested. The results showed that the membrane material significantly affects the transport and separation properties. Higher cut-off values increased permeate flux across all membranes. Ceramic membranes were more susceptible to fouling in standalone ultrafiltration, but were more effective in purifying digestate than polymeric membranes. The best results were obtained with a ceramic membrane with a 1 kDa cut-off (for example, for the integrated process and the municipal digestate, the retention rates of COD, BOD5 and DOC were 69%, 62%, and 75%, respectively).
Comparative Study on Growth and Metabolomic Profiles of Six Lactobacilli Strains by Sodium Selenite
Authors: Wang Longrui , Ju Jiasheng , Xie Huichun , Qiao Feng , Luo Qiaoyu , Zhou Lianyu
Published: September 2024

Selenium (Se) has garnered increasing attention in the field of nutrition, as it is essential for both humans and animals. Certain microorganisms can enrich inorganic selenium and convert it into organic selenium. The growth and metabolomic profiles of six lactobacilli strains exposed to 50 μg/mL of sodium selenite were performed using gas chromatography tandem time-off light mass spectrometry (GC-TOF-MS) analysis. The addition of selenium significantly increased both the population and weight of the Lacticaseibacillus rhamnosus PS5, Lbs. rhamnosus RT-B, Limosilactobacillus reuteri 3630, and Lmb. reuteri 1663 strains, while those of the other two strains decreased. A total of 271 metabolites were determined, with their concentrations ranked from highest to lowest as follows: organic acids and derivatives, oxygen compounds, lipids and lipid-like molecules, and benzenoids. In certain groups, the concentrations of serine, aspartic acid, trehalose, palmitic acid, methylthreonine, and melibiose increased significantly, whereas glucuronic acid, ribose, ornithine, and methionine were downregulated. The metabolic pathways were significantly associated with ABC transporters, glycine, serine, threonine metabolism, and aminobenzoate degradation and other pathways. Based on these findings, we concluded that the transport, absorption, assimilation, and stress response to selenium by lactobacilli in metabolomic changed. Furthermore, the metabolomic alterations among different types of lactobacilli varied primarily due to their distinct properties.
Biogenic Sulfide-Mediated Iron Reduction at Low pH
Authors: Becerra Caryl Ann , Murphy Brendan , Veldman Brittnee V. , Nüsslein Klaus
Published: September 2024

Acid mine drainage (AMD) pollutes natural waters, but some impacted systems show natural attenuation. We sought to identify the biogeochemical mechanisms responsible for the natural attenuation of AMD. We hypothesized that biogenic sulfide-mediated iron reduction is one mechanism and tested this in an experimental model system. We found sulfate reduction occurred under acidic conditions and identified a suite of sulfate-reducing bacteria (SRB) belonging to the groups Desulfotomaculum, Desulfobacter, Desulfovibrio, and Desulfobulbus. Iron reduction was not detected in microcosms when iron-reducing bacteria or SRB were selectively inhibited. SRB also did not reduce iron enzymatically. Rather, the biogenic sulfide produced by SRB was found to be responsible for the reduction of iron at low pH. Addition of organic substrates and nutrients stimulated iron reduction and increased the pH. X-ray diffraction and an electron microprobe analysis revealed that the polycrystalline, black precipitate from SRB bioactive samples exhibited a greater diversity of iron chalcogenide minerals with reduced iron oxidation states, and minerals incorporating multiple metals compared to abiotic controls. The implication of this study is that iron reduction mediated by biogenic sulfide may be more significant than previously thought in acidic environments. This study not only describes an additional mechanism by which SRB attenuate AMD, which has practical implications for AMD-impacted sites, but also provides a link between the biogeochemical cycling of iron and sulfur.
Quaternized Curcumin Derivative—Synthesis, Physicochemical Characteristics, and Photocytotoxicity, Including Antibacterial Activity after Irradiation with Blue Light
Authors: Bakun Pawel , Wysocki Marcin , Stachowiak Magdalena , Musielak Marika , Dlugaszewska Jolanta , Mlynarczyk Dariusz T. , Sobotta Lukasz , Suchorska Wiktoria M. , Goslinski Tomasz
Published: September 2024

Over the past few years, numerous bacterial strains have become resistant to selected drugs from various therapeutic groups. A potential tool in the fight against these strains is antimicrobial photodynamic therapy (APDT). APDT acts in a non-specific manner by generating reactive oxygen species and radicals, thereby inducing multidimensional intracellular effects. Importantly, the chance that bacteria will develop defense mechanisms against APDT is considered to be low. In our research, we performed the synthesis and physicochemical characterization of curcumin derivatives enriched with morpholine motifs. The obtained compounds were assessed regarding photostability, singlet oxygen generation, aggregation, and acute toxicity toward prokaryotic Aliivibrio fischeri cells in the Microtox® test. The impact of the compounds on the survival of eukaryotic cells in the MTT assay was also tested (WM266-4, WM115—melanoma, MRC-5—lung fibroblasts, and PHDF—primary human dermal fibroblasts). Initial studies determining the photocytotoxicity, and thus the potential APDT usability, were conducted with the following microbial strains: Candida albicans, Escherichia coli, Staphylococcus aureus, Streptococcus pneumoniae, and Pseudomonas aeruginosa. It was noted that the exposure of bacteria to LED light at 470 nm (fluence: 30 J/cm2) in the presence of quaternized curcumin derivatives at the conc. of 10 µM led to a reduction in Staphylococcus aureus survival of over 5.4 log.
A Quantitative Evaluation of the Influence of Chemical Variables of Biomasses of Poplar SRC Commercial Clones in Torrefaction
Authors: Rodrigues Abel Martins , Alves Ana , Graça José , Rodrigues José
Published: September 2024

This study aimed to evaluate the influence in torrefaction of the chemical structure of biomasses from nine poplar commercial SRC clones, evaluated through analytical pyrolysis. The chemical data were integrated into a dataset including LHV gain, representative of torrefaction aptitude and six chemical variables obtained through analytical pyrolysis, which were: (i) CH2Cl2 extractives; (ii) total extractives; (iii) Py-lignin; (iv) holocellulose; (v) (syringil/guaiacyl) ratio; and (vi) (pentosan/hexosan) ratio. Significant univariate and bivariate linear relations were obtained with LHV gain from torrefaction as dependent variable vs. Py-lignin, CH2Cl2 extractives and (cP/cH) ratio. Representative results were: (i) a negative correlation of −0.82 and −0.76 between LHV gain and the (pentosan/hexosan) ratio and Py-lignin, respectively, and (ii) a positive correlation of 0.79 between LHV gain and CH2Cl2 extractive amounts. Factorial and discriminant analysis allowed for clustering the tested clones in three groups, evidencing relevant influences of (S/G) ratio, Py-lignin, and, to a lesser extent, (cP/cH) ratio in the classification of these groups, clearly showing the impact of chemical variables of feedstock in torrefaction. The results contribute: (i) to the validation of use of the expedite analytical pyrolysis technique for classification of biomasses in accordance with their torrefaction aptitude and, thereby, (ii) to optimizing strategies in technological issues as diverse as poplar clone genetic breeding and modeling biomass torrefaction and pyrolysis.
Long-Lasting, Fine-Tuned Anti-Tumor Activity of Recombinant Listeria monocytogenes Vaccine Is Controlled by Pyroptosis and Necroptosis Regulatory and Effector Molecules
Authors: Olagunju Abolaji S. , Sardinha Andrew V. D. , Amarante-Mendes Gustavo P.
Published: September 2024

One of the main objectives of developing new anti-cancer vaccine strategies is to effectively induce CD8+ T cell-mediated anti-tumor immunity. Live recombinant vectors, notably Listeria monocytogenes, have been shown to elicit a robust in vivo CD8+ T-cell response in preclinical settings. Significantly, it has been demonstrated that Listeria induces inflammatory/immunogenic cell death mechanisms such as pyroptosis and necroptosis in immune cells that favorably control immunological responses. Therefore, we postulated that the host’s response to Listeria-based vectors and the subsequent induction of CD8+ T cell-mediated immunity would be compromised by the lack of regulatory or effector molecules involved in pyroptosis or necroptosis. To test our hypothesis, we used recombinant L. monocytogenes carrying the ovalbumin gene (LM.OVA) to vaccinate wild-type (WT), caspase-1/11−/−, gsdmd−/−, ripk3−/−, and mlkl−/− C57Bl/6 mice. We performed an in vivo cytotoxicity assay to assess the efficacy of OVA-specific CD8+ T lymphocytes in eliminating target cells in wild-type and genetically deficient backgrounds. Furthermore, we evaluated the specific anti-tumor immune response in mice inoculated with the B16F0 and B16F0.OVA melanoma cell lines. Our findings demonstrated that while caspase-1/11 and GSDMD deficiencies interfere with the rapid control of LM.OVA infection, neither of the KOs seems to contribute to the early activation of OVA-specific CTL responses. In contrast, the individual deficiency of each one of these proteins positively impacts the generation of long-lasting effector CD8+ T cells.
Stimulation of Arabidopsis thaliana Seed Germination at Suboptimal Temperatures through Biopriming with Biofilm-Forming PGPR Pseudomonas putida KT2440
Authors: Pandey Chandana , Christensen Anna , Jensen Martin N. P. B. , Rechnagel Emilie Rose , Gram Katja , Roitsch Thomas
Published: September 2024

This study investigated the germination response to temperature of seeds of nine Arabidopsis thaliana ecotypes. They are characterized by a similar temperature dependency of seed germination, and 10 °C and 29 °C were found to be suboptimal low and high temperatures for all nine ecotypes, even though they originated from regions with diverse climates. We tested the potential of four PGPR strains from the genera Pseudomonas and Bacillus to stimulate seed germination in the two ecotypes under these suboptimal conditions. Biopriming of seeds with only the biofilm-forming strain Pseudomonas putida KT2440 significantly increased the germination of Cape Verde Islands (Cvi-0) seeds at 10 °C. However, biopriming did not significantly improve the germination of seeds of the widely utilized ecotype Columbia 0 (Col-0) at any of the two tested temperatures. To functionally investigate the role of KT2440’s biofilm formation in the stimulation of seed germination, we used mutants with compromised biofilm-forming abilities. These bacterial mutants had a reduced ability to stimulate the germination of Cvi-0 seeds compared to wild-type KT2440, highlighting the importance of biofilm formation in promoting germination. These findings highlight the potential of PGPR-based biopriming for enhancing seed germination at low temperatures.
Effect of Environmental and Anthropic Conditions on the Development of Solanum peruvianum: A Case of the Coastal Lomas, Lima-Peru
Authors: Camel Vladimir , Quispe-Huañahue July , Felix Edwin , Ninanya-Parra Zulema , Mendoza Yngrid , Peralta-Yalta Sebastian , Pillpa Freddy , Cabello-Torres Rita
Published: September 2024

Land degradation and the effects of climate change are increasing arid lands, accelerating desertification, and leading to the loss of ecosystem services worldwide. This research focused on evaluating how human impact and environmental factors affect the development of Solanum peruvianum in its natural habitat of coastal lomas. The study was carried out in the coastal lomas of Mangomarca-Peru, where phenotypic and ecological data on the plants were collected. Information was also gathered on human impacts on the nutritional characteristics of the soils. Then, five types of organic amendments were used to improve the physical and chemical characteristics of the degraded soil, and the development and photosynthetic activity of S. peruvianum were evaluated. As a result, under the study conditions, it was found that S. peruvianum was established approximately 33.74 cm from the rocks, in a range of 300 to 650 m asl. The maximum height of the plants was 90 cm, with a stem diameter at ground level of 2 cm. S. peruvianum produced fruits between January and July, with a seed germination rate of 36% in 25 days. On the other hand, the anthropogenic impact on the soil reduced 58% of organic material (OM), 71% of nitrogen, 40% of P2O5, and 13% of K2O and increased the concentration of magnesium oxide, calcium oxide, pH, and electric conductivity (EC). The organic amendments bokashi, compost, and biochar, when mixed with the degraded soil, increased the pH, OM, N, P, and EC; however, the plants died after 25 days. On the other hand, the application of the Premix5 substrate for 100 days favored the growth of 52.84 cm and 38.29 cm in the preserved soil and 23.21 cm in the black soil mixed with blond peat, and it should be noted that the substrates presented an acid pH and EC > 0.1. Regarding photosynthetic phenotyping, S. peruvianum plants grown in their natural habitat and in Premix5 showed a higher proton flux (vH+), linear electron flow (LEF), and maximum quantum yield (Fv’/Fm’). On the contrary, they showed a lower NPQt value than plants grown in preserved and black soil mixed with blond peat.
Overexpression of Auxin/Indole-3-Acetic Acid Gene TrIAA27 Enhances Biomass, Drought, and Salt Tolerance in Arabidopsis thaliana
Authors: Iqbal Muhammad Zafar , Liang Yuzhou , Anwar Muhammad , Fatima Akash , Hassan Muhammad Jawad , Ali Asif , Tang Qilin , Peng Yan
Published: September 2024

White clover (Trifolium repens L.) is an important forage and aesthetic plant species, but it is susceptible to drought and heat stress. The phytohormone auxin regulates several aspects of plant development and alleviates the effects of drought stress in plants, including white clover, by involving auxin/indole acetic acid (Aux/IAA) family genes. However, Aux/IAA genes and the underlying mechanism of auxin-mediated drought response remain elusive in white clover. To extend our understanding of the multiple functions of Aux/IAAs, the current study described the characterization of a member of the Aux/IAA family TrIAA27 of white clover. TrIAA27 protein had conserved the Aux/IAA family domain and shared high sequence similarity with the IAA27 gene of a closely related species and Arabidopsis. Expression of TrIAA27 was upregulated in response to heavy metal, drought, salt, NO, Ca2+, H2O2, Spm, ABA, and IAA treatments, while downregulated under cold stress in the roots and leaves of white clover. TrIAA27 protein was localized in the nucleus. Constitutive overexpression of TrIAA27 in Arabidopsis thaliana led to enhanced hypocotyl length, root length, plant height, leaf length and width, and fresh and dry weights under optimal and stress conditions. There was Improved photosynthesis activity, chlorophyll content, survival rate, relative water content, endogenous catalase (CAT), and peroxidase (POD) concentration with a significantly lower electrolyte leakage percentage, malondialdehyde (MDA) content, and hydrogen peroxide (H2O2) concentration in overexpression lines compared to wild-type Arabidopsis under drought and salt stress conditions. Exposure to stress conditions resulted in relatively weaker roots and above-ground plant growth inhibition, enhanced endogenous levels of major antioxidant enzymes, which correlated well with lower lipid peroxidation, lower levels of reactive oxygen species, and reduced cell death in overexpression lines. The data of the current study demonstrated that TrIAA27 is involved in positively regulating plant growth and development and could be considered a potential target gene for further use, including the breeding of white clover for higher biomass with improved root architecture and tolerance to abiotic stress.
High-Pressure Processing of Milk and Dairy Products: Latest Update
Authors: Ozaybi Nawal
Published: September 2024

The growing global demand for minimally processed fresh foods has driven the development of innovative food processing methods. High-pressure processing (HPP), a leading non-thermal technique, has proven to be environmentally friendly, cost-effective, and versatile across various food types. Its ability to extend shelf life and ensure microbial safety is well established, particularly in dairy products. However, further research is needed to fully understand HPP’s impact on improving the digestion of milk compounds and assessing potential safety risks. Recent studies, for example, demonstrate HPP’s efficacy in enhancing the microbial safety and nutritional value of products like cheese, yogurt, and whey protein isolate. This review explores these advancements and highlights HPP’s role in improving the physicochemical, organoleptic, and functional properties of milk and dairy products.
Novel Approach of Tackling Wax Deposition Problems in Pipeline Using Enzymatic Degradation Process: Challenges and Potential Solutions
Authors: Saadon Shazleen , Rahman Raja Noor Zaliha Raja Abd , Kamarudin Nor Hafizah Ahmad , Shahruddin Sara , Shafian Siti Rohaida Mohd , Wazir Norhidayah Ahmad , Ali Mohd Shukuri Mohamad
Published: September 2024

Anthropogenic activities have led to hydrocarbon spills, and while traditional bioremediation methods are costly and time-consuming, recent research has focused on engineered enzymes for managing pollutant. The potential of enzymes for resolving wax flow problems in the petroleum industry remains unexplored. This paper offers a comprehensive review of the current state of research activities related to the bioremediation of petroleum-polluted sites and the biodegradation of specific petroleum hydrocarbons. The assayed enzymes that took part in the degradation were discussed in detail. Lipase, laccase, alkane hydroxylase, alcohol dehydrogenase, esterase, AlkB homologs and cytochrome P450 monooxygenase are among the enzymes responsible for the degradation of more than 50% of the hydrocarbons in contaminated soil and wastewater and found to be active on carbon C8 to C40. The possible biodegradation mechanism of petroleum hydrocarbons was also elucidated. The enzymes’ primary metabolic pathways include terminal, subterminal, and ω-oxidation. Next, given the successful evidence of the hydrocarbon treatment efficiency, the authors analyzed the opportunity for the enzymatic degradation approach if it were to be applied to a different scenario: managing wax deposition in petroleum-production lines. With properties such as high transformation efficiency and high specificity, enzymes can be utilized for the treatment of viscous heavy oil for transportability, evidenced by the 20 to 99% removal of hydrocarbons. The challenges associated with the new approach are also discussed. The production cost of enzymes, the characteristics of hydrocarbons and the operating conditions of the production line may affect the biocatalysis reaction to some extent. However, the challenges can be overcome by the usage of extremophilic enzymes. The combination of technological advancement and deployment strategies such as the immobilization of a consortium of highly thermophilic and halotolerant enzymes is suggested. Recovering and reusing enzymes offers an excellent strategy to improve the economics of the technology. This paper provides insights into the opportunity for the enzymatic degradation approach to be expanded for wax deposition problems in pipelines.
Experimental Study on the Transport Behavior of Micron-Sized Sand Particles in a Wellbore
Authors: Zhang Huizeng , Yin Zhiming , Ma Yingwen , Wang Mingchun , Wang Bin , Xiao Chengcheng , Yan Tie , Qu Jingyu
Published: September 2024

In the process of natural gas hydrate extraction, especially in offshore hydrate extraction, the multiphase flow inside the wellbore is complex and prone to flow difficulties caused by reservoir sand production, leading to pipeline blockage accidents, posing a threat to the safety of hydrate extraction. This paper presents experimental research on the migration characteristics of micrometer-sized sand particles entering the wellbore, detailing the influence of key parameters such as sand particle size, sand ratio, wellbore deviation angle, fluid velocity, and fluid viscosity on the sand bed height. It establishes a predictive model for the deposition height of micrometer-sized sand particles. The model’s predicted results align well with experimental findings, and under the experimental conditions of this study, the model’s average prediction error for the sand bed height is 12.47%, indicating that the proposed model demonstrates a high level of accuracy in predicting the bed height. The research results can serve as a practical basis and engineering guidance for reducing the risk of natural gas hydrate and sand blockages, determining reasonable extraction procedures, and ensuring the safety of wellbore flow.
Reclaiming Voices: We Sent Women First
Authors: Gooden Rosalind Mary
Published: September 2024

“We sent women first” could well describe Australian Baptist mission history. Australian Baptist State associations were formed in the crucible of 19th-century history, shaped by divisive issues of their British Baptist heritage and the colonial influences as each pursued an independent identity. Mission work in Bengal, India, inspired by William Carey, the BMS and BZA traditions, was the common factor, and in the six independent Australian Baptist Missionary Societies, women were sent first, starting with two from South Australia in 1882. The first man (also from South Australia) joined eleven of these women for their first ‘Convention’ in 1887.
Restoring the Spirit through the Redemption of Memory
Authors: Odor Judith
Published: September 2024

Restoration and redemption of the soul are, in biblical terms, essentially life-bringing acts. Yet even the present reality is so frequently a spirit deeply in need of renewal and new life. Memories contribute greatly to this felt need: as the psychological building blocks of an individual’s sense of self, their joy and their pain reverberate through the self, reaching deeply to shape identity and worldview. What is needed is not only the redemption of our souls but the redemption of our memories as well. Such an inner transformation would indeed prove both a restoration and a transformation, an experience now of the future kingdom in which God will reconcile all things to himself (Colossians 1:20). This article explores how the New Testament text provides a psychologically and neurologically coherent model to leverage memory formation and reconsolidation toward restoration and renewal.
Monocular 3D Multi-Person Pose Estimation for On-Site Joint Flexion Assessment: A Case of Extreme Knee Flexion Detection
Authors: Yan Guihai , Yan Haofeng , Yao Zhidong , Lin Zhongliang , Wang Gang , Liu Changyong , Yang Xincong
Published: September 2024

Work-related musculoskeletal disorders (WMSDs) represent a significant health challenge for workers in construction environments, often arising from prolonged exposure to ergonomic risks associated with manual labor, awkward postures, and repetitive motions. These conditions not only lead to diminished worker productivity but also incur substantial economic costs for employers and healthcare systems alike. Thus, there is an urgent need for effective tools to assess and mitigate these ergonomic risks. This study proposes a novel monocular 3D multi-person pose estimation method designed to enhance ergonomic risk assessments in construction environments. Leveraging advanced computer vision and deep learning techniques, this approach accurately captures and analyzes the spatial dynamics of workers’ postures, with a focus on detecting extreme knee flexion, a critical indicator of work-related musculoskeletal disorders (WMSDs). A pilot study conducted on an actual construction site demonstrated the method’s feasibility and effectiveness, achieving an accurate detection rate for extreme flexion incidents that closely aligned with supervisory observations and worker self-reports. The proposed monocular approach enables universal applicability and enhances ergonomic analysis through 3D pose estimation and group pose recognition for timely interventions. Future efforts will focus on improving robustness and integration with health monitoring to reduce WMSDs and promote worker health.
A Study on Sensitivity Improvement of the Fiber Optic Acoustic Sensing System Based on Sagnac Interference
Authors: Tang Ruixi , Zhao Hongcheng , Feng Juqin , Wang Jiang , Wang Ning , Ruan Jun , Chen Jianjun
Published: September 2024

A new pickup structure was introduced and modified to improve the resolution of the linear Sagnac optical fiber acoustic sensing system. The maximum strains corresponding to the material, diameter, wall thickness, and height of the pickups were analyzed by simulation. An aluminum cylinder with a diameter of 110 mm, a wall thickness of 3 mm, and a height of 120 mm was chosen as the basic pickup. A four-groove pickup with a vertical width of 80 mm and a horizontal width of 20 mm was introduced to improve the sensitivity of the system. The experiments showed that the average peak-to-peak sensitivity of the four-groove pickup increased by 215.54% to 106.806 mV/Pa. The improved pickup can be applied in areas to monitor the situation of invasion of the Sagnac optical fiber acoustic sensing system.