bluuebunny/crossref_metadata_2025 huggingface dataset:
THE IMPORTANCE OF BRICKS AND MORTAR IN DETERMINING THE STRENGTH OF MASONRY WALLS
Authors: Faizah Restu
Published: April 2024

Brick masonry walls are a type of construction that is in great demand, because the material is easy to obtain at a cheap price. Additionally, the process does not require any special skills. The strength of the wall is greatly influenced by the strength of the materials that make it up, namely bricks and mortar as adhesive. The role of bricks and mortar in creating wall strength needs to be investigated for their effectiveness. This research aims to obtain an overview of the effectiveness of the role of bricks or mortar in forming the compressive strength of masonry walls. To get the illustration in question, a compressive strength test of mortar, brick and masonry walls was carried out which was represented by masonry prism test objects referring to ASTM C1314-07. The test results are then compared to get an idea of the relationship between the three. From the research results it can be concluded that the compressive strength of masonry walls is determined more by the compressive strength of the bricks, but is also influenced by the compressive strength of the mortar. The higher the compressive strength of the mortar, the compressive strength of the wall will increase, but it is not significant. Therefore, the use of high strength mortar is less effective in increasing wall strength.
The Relationship Between Immune Inflammation Index and Major Cardiovascular Adverse Events in Patients with Heart Failure with Reduced Ejection Fraction.
Authors: Bektaş Osman , Akkaya Fatih
Published: April 2024

Aim: We aimed to investigate whether systemic immune inflammation index (SII) has a predictive value in predicting prognosis and major adverse cardiovascular events such as exitus, stroke and hospitalization in heart failure patients with reduced ejection fraction (HFrEF).
Methods: Patients over the age of 18 who applied to the cardiology and emergency outpatient clinics between January 2019 and January 2022 and diagnosed with HFrEF (Ejection Fraction
The Disregard for the Use of Standardized Keywords in Turkish Medical Publishing
Authors: Yıldırım Elif , Kılıç Özge
Published: April 2024

In Turkey, many medical academic journals require keywords to comply with "MeSH"(Medical Subject Headings) among their writing guidelines. However, there is currently no program or shortcut available to check the compatibility of Turkish language keywords or their English equivalents with MeSH. The purpose of this study is to determine and report the extent to which keywords used in the articles we examine are equivalent in MeSH. This study was conducted within the “DergiPark” system, which significantly supports national academic publishing. The scanning was completed between September and December 2023 by two researchers. The research focused on publications between January 1, 2022, and December 31,2022, encompassing a total of 43 journals and 1743 articles. Selection criteria for journals included a minimum of 3 years of academic publishing, issuance of at least 3 editions in 2022, printing of a minimum of 4 original articles per issue, and having English abstracts and keywords. Only those adhering to eligibility criteria in the scanned journals and publications were included in the study. Three types of articles were examined: original articles, case report, and review.
The study found that 376 of the 1743 articles were 100% compatible with MeSH. In Turkish articles, it was found that there was a higher MeSH compliance compared to English articles. As a result, the research reveals the fact that the keywords of many articles do not comply with the MeSH terms. The study emphasizes the possible consequences of disregarding MeSH indexing rules.
EVALUATION OF READABILITY AND RELIABILITY OF TURKISH WEBSITES ON LOW BACK PAIN
Authors: Akdere Emine , Karpuz Savaş , Yılmaz Ramazan , Yılmaz Halim , Solak İbrahim
Published: April 2024

OBJECTIVE: In this study, we investigated the readability and readability of Turkish websites containing information about low back pain.
MATERIALS AND METHODS: In February 2022, a search was performed by typing the word "low back pain" into Google's search engine (https://www.google.com). Commercial websites, advertising sites, chat sites, forum sites, magazine sites, sites containing only images or videos, and sites with fewer than 10 sentences were excluded from the study. Reliability scores were calculated using the mean syllable count, the mean number of words with 4 or more syllables, and Ateşman's and Bezirci-Yılmaz's scores for readability, JAMA, and DISCERN.
RESULTS: The mean number of syllables and words with 4 or more syllables was 2.78 (1.68-3.20) and 3.74 (0.36-6.31), respectively. The mean Ateşman, Bezirci-Yılmaz, and readability scores were 56.10 (29.77-100.00) and 12.80 (1.88-20.01), respectively, and the JAMA and DISCERN reliability scores were 1.06 (0-2) and 43.00 (26.00-67.00), respectively.
CONCLUSION: As a result of this study, it was found that the readability, quality, and reliability of texts on Turkish websites containing information about low back pain are at a very low level.
Global DNA Methylation analysis of imatinib resistant and sensitive K562 cells
Authors: Hekmatshoar Yalda
Published: April 2024

OBJECTIVE: Chronic myeloid leukemia (CML) is a hematological disease which is known by the presence of Philadelphia chromosome (Ph+). BCR-ABL protein is expressed by Ph+ chromosome, represents constant increased tyrosine kinase activity. Imatinib (IMA) is a tyrosine kinase inhibitor (TKI) which is utilized as a first line treatment in CML. Emergence of IMA resistance at some point of therapy leads to treatment failure. DNA methylation is considered to be the most stable epigenetic change and several studies have shown that epigenetic changes may play a role in drug resistance. We investigated the global methylation profile of IMA-sensitive K562S, IMA-resistant K562R and IMA-resistant and adherent K562R (K562R-adh) cells to determine whether epigenetic reprogramming is involved in the resistance to IMA and the change in phenotype due to this resistance.
MATERIAL AND METHODS: In this study, morphologically distinct, IMA-sensitive K562S and 5µM IMA-resistant K562R and K562R-adh in-vitro CML cell models were used to analyze the global DNA methylation profile. After DNA was isolated from the cells, global 5mC DNA methylation profiles were investigated by ELISA using equal amounts of DNA.
RESULTS: Compared to K562S, the global methylation of K562R showed an increase in DNA methylation profile, but this increase in methylation was not statistically significant. Whereas, a slight hypermethylation was observed in the DNA of the K562R-adh vs K562S and K562R-adh vs K562R which is statistically significant. We observed slight hypermethylation in IMA-resistant cells lines versus to the IMA-sensitive cell line.
CONCLUSION: Our observed differences in 5methyl-Cytosine on CpG islands (5mC) in K562S versus K562R and K562R-adh cell lines suggest that the DNA methylation alteration in resistant cells may partly contributed in phenotype switching.
CORN-COB ASH AS PARTIAL REPLACEMENT OF CEMENT FOR STABILIZATION OF LATERITE SOIL
Authors: Popoola Oluniyi Oyedeji , Adekanmi Jonathan Segun , Olulope Omolade Regina
Published: April 2024

Properties of underlying soils and borrowed soil samples are some of the key factors that determine the performance rate of roads. Most of the underlying soils possess some characteristics that make them unsuitable for use. There are available agricultural waste products in most rural settlements which can be used to treat unsuitable soils. This research examined the use of corn cob ash (CCA) as an admixture to cement on some selected geotechnical properties of laterite soil. The choice of the A-7-5 class of laterite soil is due to its general rating as poor material for subgrade and other layers of road pavement by the classification system of the American Association of State Highway and Transportation Officials (AASHTO). Cement was gradually added to the soil sample in steps of 2% from 0% to 10% by weight of the soil sample and its effect on the plasticity of the sample was examined. The addition of cement performed optimally on the soil’s plasticity at 4% which was used to form different mixtures of cement and CCA having a total sum not exceeding 4%. The additives were added to the soil sample which was subjected to laboratory tests such as compaction, California bearing ratio (CBR) and unconfined compressive strength (UCS) compacted with the efforts of 596kN/m2 and 1192kN/m2. The combination of 2% cement and 2% CCA on the soil sample improved the plasticity index and UCS properties of the soil to its optimal level while 3-1 and 4-0 cement-CCA performed optimally for CBR and compaction respectively. Thus, it was concluded that CCA performed optimally with cement at a ratio varying between 4:0 to 3:1 total percentage not exceeding 4% of the weight of the soil sample.
RECYCLING OF ASPHALT PAVEMENT AGGREGATES AND WASTE PLASTIC BOTTLES IN ADDITION TO HOT-MIX ASPHALT PRODUCTION: ADEQUATE RECYCLING RATE
Authors: Birega Tibebu , Geremew Anteneh
Published: April 2024

This study focused on the recycling of asphalt pavement aggregates and waste plastic bottles (WPB) in addition to hot-mix asphalt (HMA) production. To achieve this objective, non-probable sampling methods were used to gather samples from the study locations. Crushed stone aggregate (CSA), bitumen, mineral filler, reclaimed asphalt pavement aggregate (RAPA), and WPB were the ingredients employed based on the requirements in the standard specification for asphalt concrete production. Initially, the Marshall Stability Test was then carried out using CSA with 6% and bitumen levels of 4.0, 4.5, 5.0, 5.5, and 6% by weight of the total mix to ascertain what the bitumen content should be with RAPA with replacement rates of 10, 20, and 30% and that of WPB with 2, 6, 8, 10, 12, and 14%. In the Marshall Stability Test, which consisted of three trials of 195 samples and 60 mix designs, 45 were for the control mix and 150 for the replacement proportion. In the Marshall Stability Test, the ideal value for CSA was 5.1%; for RAPA, 5.1%; and for WPB, 7.7, 5.5, 5.4, 5.0, 5.5, and 5.4% optimum bituminous content (OBC). 20% RAPA and 10% WPB by weight of OBC in the stability-modified asphalt mix satisfies Ethiopian Road Authority (ERA) and American Society for Testing and Material (ASTM) specifications for all qualities tested. Finally, for improved asphalt mix performance, a combined 70% CSA, 20% RAPA, and 10% WPB should be used in asphalt mixes at 5.0% OBC. These experimental Marshal Stability Test findings satisfy the necessary specifications of ERA and ASTM for all tests used in HMA production. Thus, this proportion is strongly advised.
A NOVEL APPROACH OF NON-DESTRUCTIVE TESTING FOR W-BEAM GUARDRAIL DEFECT DETECTION USING SURFACE WAVE TECHNOLOGY
Authors: Yusof Zaki Muhammad Rasyadi , Abdul Hamid Syamsul Bahrin
Published: April 2024

The W-beam guardrail, alternatively referred to as a guardrail or railing, constitutes a system meticulously engineered to prevent vehicular deviation into hazardous or restricted zones. Such mechanisms are ubiquitously employed across major global thoroughfares. However, vehicular collisions can inflict considerable damage upon the W-beam, thereby compromising its structural integrity. Concurrently, subpar maintenance further exacerbates this issue, potentially transforming the W-beam from an accident prevention tool into a catalyst for mishaps. Hence, the implementation of an effective maintenance and inspection strategy is imperative. The research presented herein proposes a novel method for W-beam defect detection, leveraging surface wave technology. A pulse-echo setup has been established, employing a 10-cycle tone burst to operate at a centre frequency of 48 kHz as the transmission medium. Signals reflected from the defective specimen were subsequently analysed; however, it was observed that this technique was unable to detect all defects. Despite these limitations, the study delineates the potential to pinpoint the precise location of physical hole defects. This was achieved through the time-of-flight method and leveraging known material characteristics, such as the speed of sound, at 5 cm between the hole and transmitter. The researchers predict that this methodology, when augmented with a higher voltage and a suitable amplifier circuit for detection, could be utilized for long-distance defect detection. This opens up new avenues for ensuring the structural integrity of W-beam guardrails, thereby enhancing road safety.
VERITAS: Reflections on Harvard’s Legacy of Slavery and the New Black Female President’s Challenges to Reparations, Diversity, and Equity
Authors: Gbotokuma Zekeh S.
Published: April 2024

THE LATIN TERM VERITAS IS HARVARD UNIVERSITY’S MOTTO. IT MEANS ‘TRUTH.’ Academic freedom-based truth is what academic institutions should be all about. Regrettably, this has not always been the case because of many reasons, including but not limited to financial reasons and the politicization of the academic affairs. In this short essay, I intend to reflect on the importance of the April 26, 2022, Report of the Committee on “Harvard & the Legacy of Slavery.” It takes a great deal of moral compass, honesty, intellectual humility, a Socratic passion for the truth, leadership, and commitment to transformative change to do what Harvard’s President Lawrence S. Bacow did. Not only did he have the audacity to appoint a committee of faculty members to investigate the Ivy League institution’s controversial connection with slavery a.k.a. “America’s original sin,” but also accepted and was committed to taking reparative actions on the committee’s seven recommendations. Before delivering my reflections, it is necessary to summarize the report key findings.
Palm Oil Mill Effluent Treatment Technology using Sequencing Batch Reactor (SBR) with Oxidation Reduction Potential (ORP) Monitoring
Authors: Sanjaya Andri , Saputri Desi Riana , Damayanti Damayanti , Fahni Yunita , Auriyani Wika Atro , Mustafa Mustafa
Published: April 2024

Palm oil industries have products like Crude Palm Oil (CPO), and 70% of others contain waste. One of the wastes is the liquid waste known as Palm Oil Mill Effluent (POME). The potential of POME to be reprocessed into clean water will be profitable. One of POME's reprocessing methods is the Sequencing Batch Reactor with Aerobic Granulated Sludge (SBR-AGS), which has five main phases: filling, idling, aeration, settling, and discharge, with a cycle time of 360 minutes. The first step in using this reactor is the start-up process, a granule-forming process from some sludge that has already acclimatized. In one complete cycle, the Oxidation-Reduction Potential (ORP) parameter is used to observe the electron transfer process that shows the oxygen supply into the reactor, which enables the condition of each phase in the process to be analyzed. The trend of ORP value is constantly changing in every phase. For the idling phase, the ORP tends to decrease in a value of (-300)-(-400) mV, and for the aeration phase, it will increase in a value of (-100)-100 mV.
Extraction of Essential Oil from Moringa Oleifera Leaves Using Steam Distillation and Soxhlet Extraction Method
Authors: Haykal Muhammad Husin , Trisnawan Naufal Nafis , Wiguno Annas , Kuswandi Kuswandi
Published: April 2024

Essential oil is one of Indonesia's agro-industrial export commodities, one of which is essential oil from Moringa oleifera leaves. This study aims to study the results of the yield and analyze the essential oil content of Moringa oleifera leaves using the steam distillation method and Soxhlet extraction using ethanol and isopropyl alcohol as a solvent. The fresh feed was either directly extracted or pre-treated using an oven and then followed by several stages of extraction. The pre-treatment of fresh feed was carried out in the oven at 70°C for 1 hour. Atmospheric steam distillation was carried out at 100°C for 6 hours using 500 ml of distilled water. In the case of soxhlet extraction, the temperature was set at the boiling point of the solvent for 6 hours. Once soxhlet extraction was complete, the essential oil was separated from the solvent by distillation. After various extraction processes, the essential oils were analyzed for their compound content using GC-MS. Based on the results of the study, the highest yield of 29.04% was obtained from the treated feed using two stages of soxhlet extraction variable with ethanol as solvent.
Type 2 diabetes is linked to higher physiologic markers of effort during exercise
Authors: Huebschmann Amy G. , Scalzo Rebecca L. , Yang Xinyi , Schmiege Sarah J. , Reusch Jane E. B. , Dunn Andrea L. , Chapman Kristina , Regensteiner Judith G.
Published: April 2024

Background

People with type 2 diabetes (T2D) have lower rates of physical activity (PA) than the general population. This is significant because insufficient PA is linked to cardiovascular morbidity and mortality, particularly in individuals with T2D. Previously, we identified a novel barrier to physical activity: greater perceived effort during exercise in women. Specifically, women with T2D experienced exercise at low-intensity as greater effort than women without T2D at the same low-intensity – based on self-report and objective lactate measurements. A gap in the literature is whether T2D confers greater exercise effort in both sexes and across a range of work rates.

Objectives

Our overarching objective was to address these gaps regarding the influence of T2D and relative work intensity on exercise effort. We hypothesized that T2D status would confer greater effort during exercise across a range of work rates below the aerobic threshold.

Methods

This cross-sectional study enrolled males and post-menopausal females aged 50-75 years. Measures of exercise effort included: 1) heart rate, 2) lactate and 3) self-report of Rating of Perceived Exertion (RPE); each assessment was during the final minute of a 5-minute bout of treadmill exercise. Treadmill exercise was performed at 3 work rates: 1.5 mph, 2.0 mph, and 2.5 mph, respectively. To determine factors influencing effort, separate linear mixed effect models assessed the influence of T2D on each outcome of exercise effort, controlling for work rate intensity relative to peak oxygen consumption (%VO2peak). Models were adjusted for any significant demographic associations between effort and age (years), sex (male/female), baseline physical activity, or average blood glucose levels.

Results

We enrolled n=19 people with T2D (47.4% female) and n=18 people (55.6% female) with no T2D. In the models adjusted for %VO2peak, T2D status was significantly associated with higher heart rate (p = 0.02) and lactate (p = 0.01), without a significant association with RPE (p = 0.58).

Discussions

Across a range of low-to-moderate intensity work rates in older, sedentary males and females, a diagnosis of T2D conferred higher objective markers of effort but did not affect RPE. Greater objective effort cannot be fully attributed to impaired fitness, as it persisted despite adjustment for %VO2peak. In order to promote regular exercise and reduce cardiovascular risk for people with T2D, 1) further efforts to understand the mechanistic targets that influence physiologic exercise effort should be sought, and 2) comparison of the effort and tolerability of alternative exercise training prescriptions is warranted.
Sporadic clone Escherichia coli ST615 as a vector and reservoir for dissemination of crucial antimicrobial resistance genes
Authors: Carrera Páez Laura Camila , Olivier Martin , Gambino Anahí Samanta , Poklepovich Tomás , Aguilar Andrea Pamela , Quiroga María Paula , Centrón Daniela
Published: April 2024

There is scarce information concerning the role of sporadic clones in the dissemination of antimicrobial resistance genes (ARGs) within the nosocomial niche. We confirmed that the clinical Escherichia coli M19736 ST615 strain, one of the first isolates of Latin America that harbors a plasmid with an mcr-1 gene, could receive crucial ARG by transformation and conjugation using as donors critical plasmids that harbor blaCTX-M-15, blaKPC-2, blaNDM-5, blaNDM-1, or aadB genes. Escherichia coli M19736 acquired blaCTX-M-15, blaKPC-2, blaNDM-5, blaNDM-1, and aadB genes, being only blaNDM-1 maintained at 100% on the 10th day of subculture. In addition, when the evolved MDR-E. coli M19736 acquired sequentially blaCTX-M-15 and blaNDM-1 genes, the maintenance pattern of the plasmids changed. In addition, when the evolved XDR-E. coli M19736 acquired in an ulterior step the paadB plasmid, a different pattern of the plasmid’s maintenance was found. Interestingly, the evolved E. coli M19736 strains disseminated simultaneously the acquired conjugative plasmids in different combinations though selection was ceftazidime in all cases. Finally, we isolated and characterized the extracellular vesicles (EVs) from the native and evolved XDR-E. coli M19736 strains. Interestingly, EVs from the evolved XDR-E. coli M19736 harbored blaCTX-M-15 though the pDCAG1-CTX-M-15 was previously lost as shown by WGS and experiments, suggesting that EV could be a relevant reservoir of ARG for susceptible bacteria. These results evidenced the genetic plasticity of a sporadic clone of E. coli such as ST615 that could play a relevant transitional link in the clinical dynamics and evolution to multidrug/extensively/pandrug-resistant phenotypes of superbugs within the nosocomial niche by acting simultaneously as a vector and reservoir of multiple ARGs which later could be disseminated.
Plasma leucine-rich α-2 glycoprotein 1 in ST-elevation myocardial infarction: vertical variation, correlation with T helper 17/regulatory T ratio, and predictive value on major adverse cardiovascular events
Authors: Luo Ting , Jiang Xiaoli , Zhang Zhenzhen , Gao Ming , Wang Hao
Published: April 2024

Objective

Leucine-rich α-2 glycoprotein 1 (LRG1) promotes inflammation and myocardial injury, but its clinical role in ST-elevation myocardial infarction (STEMI) is rarely disclosed. Herein, this prospective study aimed to explore the value of plasma LRG1 at different time points to predict major adverse cardiovascular event (MACE) risk in patients with STEMI.

Methods

In total, 209 patients with STEMI were enrolled for determining plasma LRG1 at admission and on day (D)1/D7/D30 after admission via enzyme-linked immunosorbent assay, as well as for determination of peripheral blood T helper 17 (Th17) cells and regulatory T (Treg) cells by flow cytometry. In addition, plasma LRG1 was obtained from 30 healthy controls at enrollment.

Results

LRG1 was increased in patients with STEMI at admission compared with healthy controls (P < 0.001). In patients with STEMI, LRG1 varied at different time points (P < 0.001), which elevated from admission to D1, and gradually declined thereafter. LRG1 at admission was positively associated with Th17 cells (P = 0.001) and Th17/Treg ratio (P = 0.014). LRG1 at admission (P = 0.013), D1 (P = 0.034), D7 (P = 0.001), and D30 (P = 0.010) were increased in patients with MACE compared with those without. LRG1 at D7 exhibited good ability to estimate MACE risk (area under curve = 0.750, 95% confidence interval = 0.641–0.858). LRG1 at admission > 60 μg/ml (P = 0.031) and D7 > 60 μg/ml (P = 0.018) were linked with increased accumulating MACE. Importantly, LRG1 at D7 > 60 μg/ml was independently correlated with increased MACE risk (hazard ratio = 5.216, P = 0.033).

Conclusion

Plasma LRG1 increases from admission to D1 and gradually declines until D30, which positively links with Th17 cells and MACE risk in patients with STEMI.
Determinants and effects of microvascular obstruction on serial change in left ventricular diastolic function after reperfused acute myocardial infarction
Authors: Chung Hyemoon , Seo Jiwon , Kim In-Soo , Park Chul Hwan , Kim Jong-Youn , Min Pil-Ki , Yoon Young Won , Lee Byoung Kwon , Kim Tae-Hoon , Hong Bum-Kee , Rim Se-Joong , Kwon Hyuck Moon , Choi Eui-Young
Published: April 2024

Background

Although left ventricular (LV) diastolic dysfunction is more related to functional capacity after acute myocardial infarction (AMI), the determinants of LV diastolic functional change after reperfused AMI remain unknown. This study aimed to investigate the effects of microvascular obstruction (MVO) on mid-term changes in LV diastolic function after reperfused AMI.

Methods

In a cohort of 72 AMI patients who underwent successful revascularization, echocardiography and cardiovascular magnetic resonance imaging were repeated at 9-month intervals. The late gadolinium enhancement (LGE) amount, segmental extracellular volume fraction, global LV, and left atrial (LA) phasic functions, along with mitral inflow and tissue Doppler measurements, were repeated.

Results

Among the included patients, 31 (43%) patients had MVO. During the 9-month interval, LV ejection fraction (EF) and LV global longitudinal strain (GLS) were significantly improved in accordance with a decrease in LGE amount (from 18.2 to 10.3 g, p < 0.001) and LV mass. The deceleration time (DT) of early mitral inflow (188.6 ms–226.3 ms, p < 0.001) and LV elastance index (Ed; 0.133 1/ml–0.127 1/ml, p = 0.049) were significantly improved, but not in conventional diastolic functional indexes. Their improvements occurred in both groups; however, the degree was less prominent in patients with MVO. The degree of decrease in LGE amount and increase in LVEF was significantly correlated with improvement in LV-Ed or LA phasic function, but not with conventional diastolic functional indexes.

Conclusions

In patients with reperfused AMI, DT of early mitral inflow, phasic LA function, and LV-Ed were more sensitive diastolic functional indexes. The degree of their improvement was less prominent in patients with MVO.
Old woman with Sheehan's syndrome suffered severe hyponatremia following percutaneous coronary intervention: a case report and review of literature
Authors: Gao Jie , Wang Yuehai , Zhang Anqi , Pang Huihui , Wang Fei
Published: April 2024

Glucocorticoid deficiency can lead to hypoglycemia, hypotension, and electrolyte disorders. Acute glucocorticoid deficiency under stress is very dangerous. Here, we present a case study of an elderly patient diagnosed with Sheehan's syndrome, manifesting secondary adrenal insufficiency and secondary hypothyroidism, managed with daily prednisone and levothyroxine therapy. She was admitted to our hospital due to acute non-ST segment elevation myocardial infarction. The patient developed nausea and limb twitching post-percutaneous coronary intervention, with subsequent diagnosis of hyponatremia. Despite initial intravenous sodium supplementation failed to rectify the condition, and consciousness disturbances ensued. However, administration of 50 mg hydrocortisone alongside 6.25 mg sodium chloride rapidly ameliorated symptoms and elevated blood sodium levels. Glucocorticoid deficiency emerged as the primary etiology of hyponatremia in this context, exacerbated by procedural stress during percutaneous coronary intervention. Contrast agent contributed to blood sodium dilution. Consequently, glucocorticoid supplementation emerges as imperative, emphasizing the necessity of stress-dose administration of glucocorticoid before the procedure. Consideration of shorter intervention durations and reduced contrast agent dosages may mitigate severe hyponatremia risks. Moreover, it is crucial for this patient to receive interdisciplinary endocrinologist management. In addition, Sheehan's syndrome may pose a risk for coronary atherosclerotic disease.
The role of machine learning in advancing diabetic foot: a review
Authors: Guan Huifang , Wang Ying , Niu Ping , Zhang Yuxin , Zhang Yanjiao , Miao Runyu , Fang Xinyi , Yin Ruiyang , Zhao Shuang , Liu Jun , Tian Jiaxing
Published: April 2024

Background

Diabetic foot complications impose a significant strain on healthcare systems worldwide, acting as a principal cause of morbidity and mortality in individuals with diabetes mellitus. While traditional methods in diagnosing and treating these conditions have faced limitations, the emergence of Machine Learning (ML) technologies heralds a new era, offering the promise of revolutionizing diabetic foot care through enhanced precision and tailored treatment strategies.

Objective

This review aims to explore the transformative impact of ML on managing diabetic foot complications, highlighting its potential to advance diagnostic accuracy and therapeutic approaches by leveraging developments in medical imaging, biomarker detection, and clinical biomechanics.

Methods

A meticulous literature search was executed across PubMed, Scopus, and Google Scholar databases to identify pertinent articles published up to March 2024. The search strategy was carefully crafted, employing a combination of keywords such as “Machine Learning,” “Diabetic Foot,” “Diabetic Foot Ulcers,” “Diabetic Foot Care,” “Artificial Intelligence,” and “Predictive Modeling.” This review offers an in-depth analysis of the foundational principles and algorithms that constitute ML, placing a special emphasis on their relevance to the medical sciences, particularly within the specialized domain of diabetic foot pathology. Through the incorporation of illustrative case studies and schematic diagrams, the review endeavors to elucidate the intricate computational methodologies involved.

Results

ML has proven to be invaluable in deriving critical insights from complex datasets, enhancing both the diagnostic precision and therapeutic planning for diabetic foot management. This review highlights the efficacy of ML in clinical decision-making, underscored by comparative analyses of ML algorithms in prognostic assessments and diagnostic applications within diabetic foot care.

Conclusion

The review culminates in a prospective assessment of the trajectory of ML applications in the realm of diabetic foot care. We believe that despite challenges such as computational limitations and ethical considerations, ML remains at the forefront of revolutionizing treatment paradigms for the management of diabetic foot complications that are globally applicable and precision-oriented. This technological evolution heralds unprecedented possibilities for treatment and opportunities for enhancing patient care.
Lipid metabolism disorder in diabetic kidney disease
Authors: Han Yi-Zhen , Du Bo-Xuan , Zhu Xing-Yu , Wang Yang-Zhi-Yuan , Zheng Hui-Juan , Liu Wei-Jing
Published: April 2024

Diabetic kidney disease (DKD), a significant complication associated with diabetes mellitus, presents limited treatment options. The progression of DKD is marked by substantial lipid disturbances, including alterations in triglycerides, cholesterol, sphingolipids, phospholipids, lipid droplets, and bile acids (BAs). Altered lipid metabolism serves as a crucial pathogenic mechanism in DKD, potentially intertwined with cellular ferroptosis, lipophagy, lipid metabolism reprogramming, and immune modulation of gut microbiota (thus impacting the liver-kidney axis). The elucidation of these mechanisms opens new potential therapeutic pathways for DKD management. This research explores the link between lipid metabolism disruptions and DKD onset.
Impact of rural digital economy development on agricultural eco-efficiency: evidence from mainland China
Authors: Che Cheng , Yin Qi , Li Qing , Li Suhui , Zheng Huixian , Geng Xin , Zhang Shoupeng
Published: April 2024

The integration of the digital economy with rural development is of great significance as it plays a pivotal role in mitigating carbon emissions and environmental pollution in agriculture, thereby contributing to the evolution of agriculture in a green and sustainable manner. This study aims to examine the impact and mechanisms of rural digital economy development (RDED) on agricultural eco-efficiency (AEE). Specifically, based on provincial-level panel data from China spanning from 2011 to 2021, we evaluate China’s AEE by employing the super-efficiency slacks-based measure (Super SBM) model, taking into account the positive externality of agricultural carbon sinks. Then we analyze the impact and mechanisms of RDED on AEE using the two-way fixed effects model. The findings indicate that: (1) RDED significantly promotes AEE, and this conclusion remains robust even after being tested by replacing the explained variable, altering the sample interval, and including more control variables; (2) RDED can significantly drive AEE in the midwestern regions of China, but the promotion effect on the eastern region has not been fully demonstrated. Additionally, the promotion effect in southern China is greater than that in northern China; (3) agricultural science and technology investment partially mediates the impact of RDED on AEE. Moreover, agricultural science and technology innovation has a positive moderating effect on the relationship between RDED and AEE. Lastly, this study provides new evidence and policy recommendations for developing countries, such as China, to proactively facilitate the coordinated development of the rural digital economy and agricultural ecology, and attain green and sustainable ecological agriculture.
Neoadjuvant radiotherapy in ER+, HER2+, and triple-negative -specific breast cancer based humanized tumor mice enhances anti-PD-L1 treatment efficacy
Authors: Bruss Christina , Albert Veruschka , Seitz Stephan , Blaimer Stephanie , Kellner Kerstin , Pohl Fabian , Ortmann Olaf , Brockhoff Gero , Wege Anja K.
Published: April 2024

Pre-operative radiation therapy is not currently integrated into the treatment protocols for breast cancer. However, transforming immunological “cold” breast cancers by neoadjuvant irradiation into their “hot” variants is supposed to elicit an endogenous tumor immune defense and, thus, enhance immunotherapy efficiency. We investigated cellular and immunological effects of sub-lethal, neoadjuvant irradiation of ER pos., HER2 pos., and triple-negative breast cancer subtypes in-vitro and in-vivo in humanized tumor mice (HTM). This mouse model is characterized by a human-like immune system and therefore facilitates detailed analysis of the mechanisms and efficiency of neoadjuvant, irradiation-induced “in-situ vaccination”, especially in the context of concurrently applied checkpoint therapy. Similar to clinical appearances, we observed a gradually increased immunogenicity from the luminal over the HER2-pos. to the triple negative subtype in HTM indicated by an increasing immune cell infiltration into the tumor tissue. Anti-PD-L1 therapy divided the HER2-pos. and triple negative HTM groups into responder and non-responder, while the luminal HTMs were basically irresponsive. Irradiation alone was effective in the HER2-pos. and luminal subtype-specific HTM and was supportive for overcoming irresponsiveness to single anti-PD-L1 treatment. The treatment success correlated with a significantly increased T cell proportion and PD-1 expression in the spleen. In all subtype-specific HTM combination therapy proved most effective in diminishing tumor growth, enhancing the immune response, and converted non-responder into responder during anti-PD-L1 therapy. In HTM, neoadjuvant irradiation reinforced anti-PD-L1 checkpoint treatment of breast cancer in a subtype –specific manner. According to the “bench to bedside” principle, this study offers a vital foundation for clinical translating the use of neoadjuvant irradiation in the context of checkpoint therapy.
Recombinant SpTransformer proteins are functionally diverse for binding and phagocytosis by three subtypes of sea urchin phagocytes
Authors: Crow Ryley S. , Shaw Chloe G. , Grayfer Leon , Smith L Courtney
Published: April 2024

Introduction

The California purple sea urchin, Strongylocentrotus purpuratus, relies solely on an innate immune system to combat the many pathogens in the marine environment. One aspect of their molecular defenses is the SpTransformer (SpTrf) gene family that is upregulated in response to immune challenge. The gene sequences are highly variable both within and among animals and likely encode thousands of SpTrf isoforms within the sea urchin population. The native SpTrf proteins bind foreign targets and augment phagocytosis of a marine Vibrio. A recombinant (r)SpTrf-E1-Ec protein produced by E. coli also binds Vibrio but does not augment phagocytosis.

Methods

To address the question of whether other rSpTrf isoforms function as opsonins and augment phagocytosis, six rSpTrf proteins were expressed in insect cells.

Results

The rSpTrf proteins are larger than expected, are glycosylated, and one dimerized irreversibly. Each rSpTrf protein cross-linked to inert magnetic beads (rSpTrf::beads) results in different levels of surface binding and phagocytosis by phagocytes. Initial analysis shows that significantly more rSpTrf::beads associate with cells compared to control BSA::beads. Binding specificity was verified by pre-incubating the rSpTrf::beads with antibodies, which reduces the association with phagocytes. The different rSpTrf::beads show significant differences for cell surface binding and phagocytosis by phagocytes. Furthermore, there are differences among the three distinct types of phagocytes that show specific vs. constitutive binding and phagocytosis.

Conclusion

These findings illustrate the complexity and effectiveness of the sea urchin innate immune system driven by the natSpTrf proteins and the phagocyte cell populations that act to neutralize a wide range of foreign pathogens.
Single-cell transcriptomics reveals a role for pancreatic duct cells as potential mediators of inflammation in diabetes mellitus
Authors: Muñoz García Amadeo , Juksar Juri , Groen Nathalie , Zaldumbide Arnaud , de Koning Eelco , Carlotti Françoise
Published: April 2024

Introduction

Inflammation of the pancreas contributes to the development of diabetes mellitus. Although it is well-accepted that local inflammation leads to a progressive loss of functional beta cell mass that eventually causes the onset of the disease, the development of islet inflammation remains unclear.

Methods

Here, we used single-cell RNA sequencing to explore the cell type-specific molecular response of primary human pancreatic cells exposed to an inflammatory environment.

Results

We identified a duct subpopulation presenting a unique proinflammatory signature among all pancreatic cell types.

Discussion

Overall, the findings of this study point towards a role for duct cells in the propagation of islet inflammation, and in immune cell recruitment and activation, which are key steps in the pathophysiology of diabetes mellitus.
Experimental investigation of shell and helical coiled heat exchanger with Al2O3 nano-fluid with wide range of particle concentration
Authors: Shabi Omar Ali , Alhazmy Majed , Negeed El-Sayed R. , Elzoghaly Khaled O.
Published: April 2024

The purpose of this study is to experimentally enhance the heat exchange rate of the shell and helical coil tube heat exchanger by mixing water with aluminum oxide (Al2O3) nanoparticles, as well as to explore the effect of inlet thermal parameters on the performance of the heat exchanger. A test rig was constructed to investigate the influence of particle concentration, and inlet temperatures on the performance of nano-fluid. Parameters such as Nusselt number, pressure drop, performance evaluation criteria (PEC) are considered to rate the performance of the nano-particle with the heat exchanger. In this study a wider range of particle concentration is considered, which varies from 0.0%–0.75%. Experiments with and without nanoparticles are carried out under identical working conditions. By analyzing the experimental data, it was found that nanoparticles significantly improve the coefficient of heat transfer inside the helically coiled tube. From sensitivity analysis, it is obseerved that there is a slight decrease in Nusselt number of the nano-fluid with increase in inlet temperatures of the nano-fluid and the cooling water. Furthermore, it is concluded that an 8.5% increase in PEC value is observed with increase in particle concentration from 0.15% to 0.75%.
Lung function benefits of traditional Chinese medicine Qiju granules against fine particulate air pollution exposure: a randomized controlled trial
Authors: Chen Rucheng , Zhang Lu , Gu Weijia , Li Ran , Hong Huihua , Zhou Linshui , Zhang Jinna , Wang Yixuan , Ni Ping , Xu Shuqin , Wang Zhen , Sun Qinghua , Liu Cuiqing , Yang Junchao
Published: April 2024

Introduction

Multiple targets are considered as the causes of ambient fine particulate matter [aerodynamic diameters of < 2.5 μm (PM2.5)] induced lung function injury. Qiju granules are derived from the traditional Chinese medicine (TCM) formula known as Qi-Ju-Di-Huang-Wan (Lycium, Chrysanthemum, and Rehmannia Formula, QJDHW), which has been traditionally used to treat symptoms such as cough with phlegm, dry mouth and throat, and liver heat. This treatment approach involves attenuating inflammation, oxidative stress, and fibrosis response. This study investigated the effects of Qiju granules on protecting lung function against PM2.5 exposure in a clinical trial.

Methods

A randomized, double-blinded, and placebo-controlled trial was performed among 47 healthy college students in Hangzhou, Zhejiang Province in China. The participants were randomly assigned to the Qiju granules group or the control group based on gender. Clinical follow-ups were conducted once every 2 weeks during a total of 4 weeks of intervention. Real-time monitoring of PM2.5 concentrations in the individually exposed participants was carried out. Data on individual characteristics, heart rate (HR), blood pressure (BP), and lung function at baseline and during the follow-ups were collected. The effects of PM2.5 exposure on lung function were assessed within each group using linear mixed-effect models.

Results

In total, 40 eligible participants completed the scheduled follow-ups. The average PM2.5 level was found to be 64.72 μg/m3 during the study period. A significant negative correlation of lung function with PM2.5 exposure concentrations was observed, and a 1-week lag effect was observed. Forced expiratory volume in one second (FEV1), peak expiratory flow (PEF), maximal mid-expiratory flow (MMEF), forced expiratory flow at 75% of forced vital capacity (FVC) (FEF75), forced expiratory flow at 50% of FVC (FEF50), and forced expiratory flow at 25% of FVC (FEF25) were significantly decreased due to PM2.5 exposure in the control group. Small airway function was impaired more seriously than large airway function when PM2.5 exposure concentrations were increased. In the Qiju granules group, the associations between lung function and PM2.5 exposure were much weaker, and no statistical significance was observed.

Conclusion

The results of the study showed that PM2.5 exposure was associated with reduced lung function. Qiju granules could potentially be effective in protecting lung functions from the adverse effects of PM2.5 exposure.

Clinical Trial Registration

identifier: ChiCTR1900021235.
Cyclic cryotherapy with vitamin D facilitates early rehabilitation after total knee arthroplasty
Authors: Li Fulin , Mo Yingrong , Huang Xiao , Sun Ke , Li Baichuan , Yin Dong
Published: April 2024

Objective

This study aimed to evaluate the efficacy of cyclic cryotherapy and vitamin D administration on early rehabilitation after total knee arthroplasty (TKA), as its efficacy remains unclear.

Methods

We divided 150 patients (three groups) who underwent TKA into those treated with or without cyclic cryotherapy and vitamin D.

Results

Compared with patients who did not receive cyclic cryotherapy, those who received postoperative cyclic cryotherapy and vitamin D supplementation had significantly higher American Knee Society Scores (AKSS) on postoperative day (POD) 7 and at 1 month postoperatively; higher visual analogue scale (VAS) values on POD1–3 and POD7; reduced thigh swelling on POD3 and POD7; increased range of motion (ROM) on POD3, POD7, and at 1 month postoperatively; and reduced postoperative length of stay (PLOS). However, no significant difference in patient satisfaction was observed between the patient groups. At 1 and 3 months postoperatively, patients administered cyclic cryotherapy and vitamin D had significantly higher AKSS, ROM, and vitamin D levels than those who did not receive vitamin D. No perioperative complications such as surgical site infection, skin frostbite, or vitamin D intoxication were observed.

Conclusion

Cyclic cryotherapy post-TKA had short-term advantages in terms of AKSS, VAS, thigh swelling, ROM, PLOS, and accelerated rehabilitation, but did not improve patient satisfaction. Cyclic cryotherapy combined with vitamin D improved AKSS and ROM at 1 and 3 months postoperatively.
Exercise interventions for nonspecific low back pain: a bibliometric analysis of global research from 2018 to 2023
Authors: Zang Wanli , Yan Jin
Published: April 2024

Objective

This study aims to explore global research trends on exercise interventions for nonspecific low back pain from 2018 to 2023 through bibliometric analysis.

Methods

A systematic search was conducted in the Web of Science Core Collection database to select relevant research articles published between 2018 and 2023. Using CiteSpace and VOSviewer, the relationships and impacts among publications, different countries, journals, author groups, references, and keywords were analyzed in depth.

Results

The bibliometric analysis included 4,896 publications, showing a trend of initial growth followed by a decline. At the national level, the United States made the most significant contributions in this field. The journal “Lancet” had three of the top 10 most-cited articles, with an average citation count of 306.33, and an impact factor reaching 168.9 in 2023. The analysis also revealed that “disability,” “prevalence,” and “management” were high-frequency keywords beyond the search terms, while “rehabilitation medicine,” “experiences,” and “brain” emerged as new hotspots in the research.

Conclusion

This study reveals the global trends in research on exercise interventions for nonspecific low back pain over the past 5 years and highlights potential research frontiers in the field. These findings provide a solid foundation for focusing on key issues, potential collaboration directions, and trends in research development in the future, offering valuable references for further in-depth studies.
Horizontal gene transfer in plant microbiomes: integrons as hotspots for cross-species gene exchange
Authors: Ghaly Timothy M. , Gillings Michael R. , Rajabal Vaheesan , Paulsen Ian T. , Tetu Sasha G.
Published: April 2024

Plant microbiomes play important roles in plant health and fitness. Bacterial horizontal gene transfer (HGT) can influence plant health outcomes, driving the spread of both plant growth-promoting and phytopathogenic traits. However, community dynamics, including the range of genetic elements and bacteria involved in this process are still poorly understood. Integrons are genetic elements recently shown to be abundant in plant microbiomes, and are associated with HGT across broad phylogenetic boundaries. They facilitate the spread of gene cassettes, small mobile elements that collectively confer a diverse suite of adaptive functions. Here, we analysed 5,565 plant-associated bacterial genomes to investigate the prevalence and functional diversity of integrons in this niche. We found that integrons are particularly abundant in the genomes of Pseudomonadales, Burkholderiales, and Xanthomonadales. In total, we detected nearly 9,000 gene cassettes, and found that many could be involved in plant growth promotion or phytopathogenicity, suggesting that integrons might play a role in bacterial mutualistic or pathogenic lifestyles. The rhizosphere was enriched in cassettes involved in the transport and metabolism of diverse substrates, suggesting that they may aid in adaptation to this environment, which is rich in root exudates. We also found that integrons facilitate cross-species HGT, which is particularly enhanced in the phyllosphere. This finding may provide an ideal opportunity to promote plant growth by fostering the spread of genes cassettes relevant to leaf health. Together, our findings suggest that integrons are important elements in plant microbiomes that drive HGT, and have the potential to facilitate plant host adaptation.