bluuebunny/crossref_metadata_2025 huggingface dataset:
Impregnation Nickel on Mesoporous ZSM-5 Templated Carbons as a Candidate Material for Hydrogen Storage
Authors: Ediati Ratna , Mukminin Amirul , Widiastuti Nurul
Published: April 2017

A series of carbon with ZSM-5 mesoporous (ZMC) and Ni/ZMC ZSM-5 templates were synthesized and gravimetrically known its adsorption capacity to H2 gases. ZMC carbon was synthesized using a hard template method at a carbonation temperature of 900 oC with sucrose as a source of carbon. A mesoporous ZSM-5 template was synthesized by using a hydrothermal method at an aging and crystallization temperature of respectively 60oC and 150oC for as long as 24 hours. The characterized result of ZMC carbon using XRD powder has shown a diffracted pattern of amorphous carbon. Impregnating Ni with ZMC carbon each resulted in 5%, 15% and 25% of weight, which in turn also resulted in a diffractogram pattern of Ni/ZMC-5, Ni/ZMC-15, and Ni/ZMC-25, where each patterns were uniform and had no additional peaks. The results of SEM-EDAX showed that Ni had been successfully impregnated on the surface of ZMC carbon with a random morphology surface of ZMC carbon. The isothermal results of adsorption – desorption of N2 showed that the larger the Ni loading on the supporting ZMC carbon, the smaller the surface area of the ZMC specific carbon. The distribution of the pore size that was counted by using the BJH method, which was applied to all the samples, was as big as 3,8 nm. The adsorption capacity of H2 was measured by using the gravimetry method at ambient temperature for Ni/ZMC-5, Ni/ZMC-15, and Ni/ZMC-25, which each gave a weight percentage of 0,331%; 0,663% and 0,649% respectively, whilst the ZMC carbon has a weight of 2,18%.
Application of Ion-Exchange Resin Column for Basic Development of Strontium-90/Yttrium-90 Generator for Preparation of Radiopharmaceutical Therapy
Authors: Muchtaridi Muchtaridi , Kamal Elvan , Subarnas Anas , Mutalib Abdul
Published: April 2017

The aim of this study is to examine the possible use of ion-exchange resin on Dowex AG 50x8 in separating Y-90 from Sr-90 which then could be applied in the basic of manufacture of producing Sr-90/Y-90 generator for the production of radiopharmaceutical cancer therapy. The findings showed that a distributive coefficient of Sr-90 and Y-90 with HCl 6 N solvent showed that distributive coefficient of Sr-90 in cation-exchange resin on Dowex 50x8 was greater than Y-90. Y-90 in HCl 6 N solvent was more easily eluted, while Sr-90 was bound to resin.. In conclusion, resin on Dowex AG 50x8 with HCl 6 N elution solvent might be able to separate Y-90 from Sr-90 as the basis for Sr-90/Y-90 generator.
Development of Quality Control Method for Glucofarmaka Antidiabetic Jamu by HPLC Fingerprint Analysis
Authors: Habibie Hanifullah , Heryanto Rudi , Rafi Mohamad , Darusman Latifah Kosim
Published: April 2017

Herbal medicines become increasingly popular all over the world for preventive and therapeutic purposes. Quality control of herbal medicines is important to make sure their safety and efficacy. Chromatographic fingerprinting has been accepted by the World Health Organization as one reliable strategy for quality control method in herbal medicines. In this study, high-performance liquid chromatography fingerprint analysis was developed as a quality control method for glucofarmaka antidiabetic jamu. The optimum fingerprint chromatogram were obtained using C18 as the stationary phase and linear gradient elution using 10–95% acetonitrile:water as the mobile phase within 60 minutes of elution and detection at 210 nm. About 20 peaks were detected and could be used as fingerprint of glucofarmaka jamu. To evaluate the analytical performance of the method, we determined the precision, reproducibility, and stability. The result of the analytical performance showed reliable results. The proposed method could be used as a quality control method for glucofarmaka antidiabetic jamu and also for its raw materials.
Investigation of Effect of Adding Hydrophobically Modified Water Soluble Polymers on the Structure and Viscosity of Anionic Vesicle Dispersion
Authors: Sandjaja Marco , Lestari Maria Lucia Ardhani Dwi
Published: April 2017

This present study was conducted to investigate the effect of adding hydrophobically modified end-capped (HM) polymers with various polyethylene oxide (PEO) chain lengths on the structure and viscosity of anionic vesicles dispersion. A pronounced increase in viscosity was observed upon adding small amount of such polymers. Based on the dynamic light scattering (DLS) and small angle neutron scattering (SANS) analysis, 10 to 30 polymer molecules per vesicles can reach maximum viscosity and where polymer molecules can interconnect the vesicles without disrupting their structure. In addition, the kinetic stability of the vesicle dispersion also enhanced. From the measurement of the electrical conductivity of the dispersion, it was observed that the presence of the PEO and polypropylene oxide (PPO) group could induce the permeability of the vesicle membrane by altering their internal structure. Controlling viscosity of vesicles dispersion without changing its structure is useful for the further application of vesicles system such as in drug delivery, cosmetics and biomedical.
Older Patients’ Perspectives on Managing Complexity in CKD Self-Management
Authors: Bowling C. Barrett , Vandenberg Ann E. , Phillips Lawrence S. , McClellan William M. , Johnson Theodore M. , Echt Katharina V.
Published: April 2017

Background and objectives

Patients with CKD are asked to perform self-management tasks including dietary changes, adhering to medications, avoiding nephrotoxic drugs, and self-monitoring hypertension and diabetes. Given the effect of aging on functional capacity, self-management may be especially challenging for older patients. However, little is known about the specific challenges older adults face maintaining CKD self-management regimens.

Design, setting, participants, & measurements

We conducted an exploratory qualitative study designed to understand the relationship among factors facilitating or impeding CKD self-management in older adults. Six focus groups (n=30) were held in August and September of 2014 with veterans≥70 years old with moderate-to-severe CKD receiving nephrology care at the Atlanta Veterans Affairs Medical Center. Grounded theory with a constant comparative method was used to collect, code, and analyze data.

Results

Participants had a mean age (range) of 75.1 (70.1–90.7) years, 60% were black, and 96.7% were men. The central organizing concept that emerged from these data were managing complexity. Participants typically did not have just one chronic condition, CKD, but a number of commonly co-occurring conditions. Recommendations for CKD self-management therefore occurred within a complex regimen of recommendations for managing other diseases. Participants identified overtly discordant treatment recommendations across chronic conditions (e.g., arthritis and CKD). Prioritization emerged as one effective strategy for managing complexity (e.g., focusing on BP control). Some patients arrived at the conclusion that they could group concordant recommendations to simplify their regimens (e.g., protein restriction for both gout and CKD).

Conclusions

Among older veterans with moderate-to-severe CKD, multimorbidity presents a major challenge for CKD self-management. Because virtually all older adults with CKD have multimorbidity, an integrated treatment approach that supports self-management across commonly occurring conditions may be necessary to meet the needs of these patients.

Podcast

This article contains a podcast at https://www.asn-online.org/media/podcast/CJASN/2017_04_07_Bowling.mp3
ROASTED CASHEW NUT KERNEL (ANACARDIUM OCCIDENTALE L.) SUPPLEMENTATION IMPROVES HEPATIC AND RENAL STATUS BUT NOT LIPID PROFILE IN RATS
Authors: Famurewa Ademola Clement , Abi Innocent , U Eru Emmanuel
Published: April 2017

ABSTRACTObjective: Although studies suggest that nut consumption is associated with a variety of beneficial health outcomes, however, there is a dearth of datain the literature to document this effect for roasted cashew nut kernel (RCNK). Our objective was to determine whether dietary consumption of RCNKcould improve lipid profile, hepatic and renal status in rats.Methods: A total of 24 rats were randomly divided into four groups: A control and three experimental groups fed with roasted cashew nutsupplementeddietatdifferentconcentrationsfor28consecutivedays.Aftertheexperimentalperiod,ratswereanesthetized withether andretroorbitalbloodsampleswerewithdrawn.Serumsamples wereobtained toanalyzelipid profile,markersof oxidativestress,hepatic andrenalstatususingstandardmethods.Results: The supplemented diet significantly decreased the liver function parameters in rats. All the concentrations of RCNK in diets significantlyreduced serum creatinine and urea levels. However, total cholesterol and low-density lipoprotein-cholesterol (LDL-C) were significantly increased,whereas oxidative stress markers and malondialdehyde were improved by the supplemented diet, although insignificantly, as compared with thecontrol.Conclusions: These results suggest that RCNK may have beneficial health effects on the liver and kidney status although marked improvement wasnot demonstrated in oxidative stress markers. However, the significant increase in serum total and LDL-C indicates the need for further studies.Keywords: Roasted cashew nut, Anacardium occidentale, Lipid profile, Liver enzymes, Oxidative stress.
ANTIBACTERIAL ACTIVITY OF CHITOSAN AND HYDROLYZED COCONUT OIL AND THEIR COMBINATION AGAINST BACILLUS CEREUS AND ESCHERICHIA COLI
Authors: Silalahi Jansen , Situmorang Petrika , Patilaya Popi , Ce Silalahi Yosy
Published: April 2017

ABSTRACTObjective: The purpose of this study was to evaluate the antibacterial activity of chitosan, hydrolyzed coconut oil and their combination againstBacillus cereus and Escherichia coli.Methods: The materials used in this study were powder of chitosan (obtained from prawn shell produced by Laboratory of Research Centre FMIPAUniversity of Sumatera Utara) and virgin coconut oil (VCO) product of Siti Nurbaya-Indonesia. VCO was partially hydrolyzed by Lipozyme TL IM(active at sn-1,3 position) and the result called hydrolyzed virgin coconut oil (HVCO). The bacteria used in this study were B. cereus and E. coli. Theantibacterial activity of chitosan in 1% acetic acid and HVCO in dimethylsulfoxide was tested by Kirby–Bauer agar diffusion method using paper discwith diameter of 6 mm.Results: The results showed that the minimum inhibitory concentration of chitosan against B. cereus and E. coli is at concentration of 0.05% withinhibition zone diameter of 6.86 mm and 7.56 mm, respectively. MIC of HVCO against B. cereus is at concentration of 0.25% with inhibition zonediameter of 6.40 mm, and against E. coli is at a concentration of 0.50% with inhibition zone diameter of 6.20 mm. The inhibition zone diameter ofchitosan 0.05% and HVCO 0.25% in combination against B. cereus is 8.33 mm which is higher than half the sum of chitosan 0.05% and HVCO 0.25%(6.63 mm). The inhibition zone diameter of chitosan 0.05% and 0.5% HVCO in combination against E. coli is 8.53 mm which is higher than half thesum of chitosan 0.05% and HVCO 0.5% (6.53 mm).Conclusion: The findings of this study indicate that chitosan is more antibacterial than HVCO, and the interaction between chitosan and HVCO incombination demonstrated to be synergistic against B. cereus and E. coli.Keywords: Antibacterial, Chitosan, Coconut oil, Combination, Bacillus cereus, Escherichia coli.®
INFLUENCE OF LOVASTATIN ON PHARMACOKINETICS AND PHARMACODYNAMICS OF GLIPIZIDE IN HEALTHY AND STREPTOZOTOCIN - INDUCED DIABETIC RATS: INVOLVEMENT OF P-GLYCOPROTEIN INHIBITION
Authors: Kadimpati Kishore Kumar , Sammeta Vanishree , Pingili Ravindra Babu , Sanneboina Dr. Sujatha
Published: April 2017

ABSTRACTThis study evaluated the effect of lovastatin (Lov) on the pharmacokinetics and pharmacodynamics of glipizide (Gpz) in healthy and streptozotocininduceddiabeticrats.In singledose study(SDS), blood samples werecollectedonthe 1st day, whereas in multiple dose study on the 15 day at0-12 hrs. Lov significantly altered the pharmacokinetic parameters at the dose of 15 mg/kg in SDS and multiple dose study. The C of Gpz wasincreased from 2.97 to 8.38 and 9.87 to 24.58 ng/mL in healthy and diabetic rats, respectively, in multiple dose study. Rat everted sacs were used tostudy the transport of Gpz in the presence of Lov and verapamil (P-glycoprotein [P-gp] inhibitor). The transport of Gpz from mucosal to the serosalsurface was significantly increased from 4.32 to 5.65 and 6.02 µg/mL in the presence of Lov and verapamil, respectively. The interaction between Lovand Gpz is due to P-gp and CYP2C9 inhibition.Keywords: Diabetes, Dyslipidemia, Glipizide, Lovastatin, P-glycoprotein.maxth
ANTI-INFLAMMATORY STUDY OF ANREDERA CORDIFOLIA LEAVES AND CENTELLA ASIATICA HERBS AND ITS COMBINATIONS USING HUMAN RED BLOOD CELL-MEMBRANE STABILIZATION METHOD
Authors: Sutrisno Entris , Adnyana I Ketut , Yulinah Sukandar Elin , Fidrianny Irda , Aligita Widhya
Published: April 2017

ABSTRACTObjective: Inflammation is body reactions in response to tissue injury and infection. In 2011, non-steroidal anti-inflammatory drug (NSAID) was thehighest demand drug in Indonesia. However, long-term treatment using NSAID can cause several side effects to cardiovascular and digestive system.This research aimed to investigate anti-inflammatory properties of binahong leaves (Anredera cordifolia) and pegagan herbs (Centella asiatica).Methods: Ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs was evaluated for its anti-inflammatory properties using human redblood cell (RBC) – membrane stabilization assay. The extract concentrations used in this study was 100, 200, 400, and 800-ppm, and apigenin andasiaticoside concentration were 1, 2, 3, 4, 5, 6, 10, and 100 ppm. Diclofenac natrium (DN) was used as a standard drug.Results: The results showed that A. cordifolia extract (ACE) alone, C. asiatica extract (CAE) alone, and the combination of ACE and CAE could inhibit thehemolysis of RBC in hypotonic solution. The optimum concentration for ACE alone was 100 ppm; for CAE alone was 400 ppm; and for the combinationof ACE and CAE was 50 ppm and 50 ppm, respectively. Apigenin and asiaticoside in concentration of 1-10 ppm showed more than 97% inhibition ofhemolysis. DN as a standard drug showed optimum inhibition at concentration of 400 ppm.Conclusion: The ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs showed anti-inflammatory activity, both as a single treatment or ascombinations, and apigenin and asiaticoside were responsible for anti-inflammatory activity in C. asiatica.Keywords: Anti-inflammation, Human red blood cell – membrane stabilization, Anredera cordifolia, Centella asiatica.
STUDY OF THE POSSIBLE ANTIHYPERTENSIVE AND HYPOLIPIDEMIC EFFECTS OF AN HERBAL MIXTURE ON L-NAME-INDUCED HYPERTENSIVE RATS
Authors: Aboubaker Doha , E El-gengaihi Souad , Abdel Salam Mouchira , Mm Ibrahim Bassant , El-batran Seham
Published: April 2017

ABSTRACTObjective: Hypertension is a chronic medical condition. Diet can improve blood pressure control and decrease the risk of health complication.Methods: In this study, four plants: Roselle, Marjoram, Chamomile, and Doum were extracted by water. Equal portions of them were mixed. Lethaldose 50% of the mixture was assayed; the dose which did not cause any mortality was 266.94 mg/100 g body weight. Animals were classified into fivegroups: Negative control group, positive control group where hypertension was induced by L-name, two groups treated with two doses of the mixture,and a group treated with prazosin as a standard treatment. Treatment of hypertensive rats continued for 4 successive weeks.Results: Treatment with the mixture showed a significant reduction in blood pressure of hypertensive rats, as well as serum cholesterol, low-densitylipoprotein-cholesterol, and urea levels when compared to positive control group.Conclusion: The results obtained suggest that the aqueous extract is efficient as an antihypertensive and hypolipidemic agent.Keywords: Rats, Aqueous extract, Hypertension, Hyperlipidemia, L-name.
CLINICO-PATHOLOGICAL CHARACTERISTICS OF LUPUS NEPHRITIS IN EASTERN ZONE OF INDIA: A SINGLE TERTIARY CENTER EXPERIENCE
Authors: Panda Smaraka Ranjan , Kar Chittaranjan , Sahu Prasant Kumar , Rout Sashibhusan , Mohanty Bishwaranjan , Behera Tapas Ranjan , Behera Sudeshna
Published: April 2017

ABSTRACTObjective: Lupus nephritis (LN) is the most common and serious manifestation of systemic lupus erythematosus (SLE) and an important cause ofmorbidity and mortality. Although diagnosis of LN is straight forward in a patient with SLE and proteinuria, and active urine sediment and perhapsrenal insufficiency, still renal biopsies are required at diagnosis to enable classification of nephritis severity, to provide prognostic information, and toguide treatment. Hence, the objective of this study is to determine the frequency of distribution of different classes of LN based on renal biopsy reportsand to correlate it with various laboratory findings.Methods: Retrospective study was done in all patients with LN who had at least one representative renal biopsy and evaluated in NephrologyDepartment of SCB Medical College, Cuttack, in 6-month duration. Various laboratory values were recorded and correlated with histopathologicallupus classifications.Results: Out of 35 patients enrolled, 33 (94.28%) were females and 2 (5.71%) were males. Mean age was 27.53±12.26 years. Majority of cases belongto Class IV followed by Class V. Patients of Class IV LN have a significantly low hemoglobin level. Similarly, serum urea and creatinine are higher inGroup IV than other groups, and serum creatinine was found to be significant. 24 hrs urinary protein excretion has a significant correlation with theclasses of LN.Conclusion: This study suggests some meaningful correlation between laboratory findings and histopathological lupus classification. This study alsosuggests that renal biopsies are still beneficial for better evaluation of renal status and determination of LN classes.Keywords: Clinico-pathological correlation, Lupus nephritis, Biopsy.
ANALGESIC ACTIVITY OF HYDROALCOHOLIC EXTRACT OF AERIAL PARTS OF MALVASTRUM COROMANDELIANUM
Authors: Semwal Bhupesh Chander , D Kalyani , B Rohit
Published: April 2017

ABSTRACTObjective: The aim of this study is to explore the antinociceptive property of hydroalcoholic extract of Malvastrum coromandelianum. Pain is anunpleasant sensory and emotional experience associated with actual and potential tissue damage. Various types of pain are seen in humans, somatic,visceral, and neuropathic pain. M. coromandelianum belongs to Malvaceae family. Traditionally, it is used as an emollient, anti-inflammatory, analgesic,and antidysenteric.Methods: The antinociceptive effects of hydroalcoholic extract of M. coromandelianum (HEMC) were evaluated by tail-flick, hot-plate, and acetic acidinduced writhing method. Rats were divided into four groups. The first group which served as control was administered with aqueous 1% tragacanthsuspension. The second group received standard drug, morphine (5 mg/kg) orally as a suspension. Third and fourth group received HEMC (200 and400 mg/kg) and served as test drug treatment group, 30 minutes after treatment the reaction time and number of writhes was noted.Results: HEMC produced significant antinociceptive effects against thermally induced pain. In tail-flick method, the peak effect of HEMC 400 mg/kgwas shown at 60 minutes which is nearly equal to the peak effect of morphine 5 mg/kg. In hot-plate method, the HEMC showed significant analgesiceffect up to 3 hrs after the treatment, whereas morphine showed significant effect up to 6 hrs.Conclusion: On the basis of finding, it may conclude that the HEMC shows its central analgesic action probably through inhibition of central painreceptors, whereas peripheral analgesic effect may be mediated by prostaglandin inhibition.Keywords: Pain, Analgesia, Hydroalcoholic extract of Malvastrum coromandelianum, Morphine.
EFFECT OF PHARMACOLOGICAL DOSES OF GARLIC AND OMEGA 3 ON GASTRIC LESIONS INDUCED BY ETHANOL IN MICE
Authors: Jaccob Ausama Ayoob
Published: April 2017

ABSTRACTObjective: This work was designed to investigate and compare the possible protective effect of pharmacological doses of garlic and omega 3 againstgastric lesions induced by ethanol in mice.Methods: A total of 30 mice involved in the study were divided into five groups with 6 mice for each. Groups 1 (ulcer control) and 2 (normal control),Groups 3 (positive ulcer control treated with ranitidine 50 mg/kg), Groups 4 and 5 treated with garlic oil and omega 3 oil at doses 200 and 150 mg/kg,respectively. All groups treated orally by gastric gavage once daily for 14 days before starting gastric ulcer (GU) induction process by absolute ethanoladministration.Results: A treatment with garlic and omega 3 ameliorated the severity of gastric ulceration evidenced by reduced ulcer index area, increase %gastroprotection, increase mucus content, reduced erosions, and necrosis.Conclusion: Oral administration of pharmacological doses of garlic oil and omega 3 oil shows significant gastroprotection against GU models inducedby absolute ethanol confirmed by biochemical and histological data.Keywords: Gastric ulcer, Garlic, Omega 3, Gastroprotection.
IN VIVO HEPATOPROTECTIVE ACTIVITY OF CASSIA AURICULATA POLYMER NANOSPHERES CONTAINING SILYMARIN
Authors: Sagar Avula Keerthi , Devala Rao G.
Published: April 2017

ABSTRACTObjective: Hepatoprotective activity of herbal drugs has an importance in the treatment of hepatic disorders, but pharmaceutically engineeredproducts are not evolved properly to cure the liver disorders properly based on the demand and also route, target the appropriate site of action.Methods: In our present work, we have been developed a new formulation that possesses a unique nature and site specificity for targeting the diseasestate. Here, we examined hepatoprotective activity of Cassia auriculata polymer nanospheres containing silymarin against carbon tetrachlorideinducedhepatotoxicityinratsusingat50mg/kgand100mg/kgbodyweightdoselevels,andwehavebeen observedthat enzymeactivitiesof serumglutamateoxaloacetatetransaminase,serum glutamatepyruvatetransaminase,alkalinephosphate,totalprotein,albumin, globulin,totalcholesterol,high-densitylipoprotein(HDL), glutathione(GSH), and totalbilirubin wereanalyzed.Results: C. auriculata polymer nanospheres and silymarin produced significant (p<0.001) hepatoprotective effect by decreasing the activity of serumenzymes, bilirubin, total cholesterol, and increased levels of HDL, total protein, albumin, globulin, and tissue GSH.Conclusion: From these results, it was concluded that of C. auriculata polymer nanospheres would protect the liver cells from carbon tetrachloridefrom liver diseases.Keywords: Hepatoprotective activity, Silymarin, Cassia auriculata, Nanospheres.
SUBCHRONIC TOXICITY TEST OF COMBINATION ETHANOL EXTRACT OF DETAM 1 SOYBEAN (GLYCINE MAX L. MERR) AND JATI BELANDA (GUAZUMA ULMIFOLIA LAMK) TOWARD FUNCTION, WEIGHT, AND HISTOPATHOLOGICAL OF WISTAR RAT LIVER
Authors: Hidayat Meilinah , Prahastuti Sijani , Dewi Estherolita , Safitri Dewi , Rahmawati Siti Farah , Soemardji Andreanus Andadja
Published: April 2017

ABSTRACTObjective: As an antiobesity therapy, combination extracts of Detam 1 soybean and Jati Belanda will be consumed for a long time; therefore, theirtoxicities to the liver need to be investigated. To determine the effect of subchronic toxicity test of combination of ethanol extract of Detam 1 soybean(EEDS) and ethanol extract of Jati Belanda (EEJB) on liver function with parameters: Alanine transaminase (ALT), macroscopic, and histopathologicalof liver.Methods: This study was conducted on 120 Wistar rats (60 males and 60 females), 90 days (treatment group) and 120 days (satellite group). Ratswere divided into six treatment groups (3 test materials, 1 control, and 2 satellites); each group included 10 males and 10 females.Results: ALT levels of treatment groups (low dose, medium, and high), both males and females were lower than the control group (p<0.05). Thetreatment groups demonstrated a good effects effect on liver function. Liver weight of all groups showed no significant difference compared with thecontrol group (p>0.05). Results of histopathological score interpretation of male and female liver rats of low dose groups were not disturbed; middledose groups were slightly disturbed and high dose groups were damaged. Satellite high doses of male groups were disrupted, while female groupswere not.Conclusion: The combination of EEDS and EEJB has a good effect on liver function, did not lead to change organ weight and at low doses did not causerenal histopathology damage in rats after 90 days administration.Keywords: Combination of soybean Jati Belanda, Toxicity subchronic test, Function, Weight, Histopathology, Liver.
XPERT MYCOBACTERIUM TUBERCULOSIS/RIFAMPICIN ASSAY: A BOON IN TUBERCULOSIS DIAGNOSTICS
Authors: Bhat Sevitha , Ramamurthy Kavya , Shenoy Shalini , Rangnekar Aseem
Published: April 2017

ABSTRACTObjectives: Mycobacterium tuberculosis (MTB) remains one of the most significant causes of mortality and morbidity in developing countriesespecially India. India has the highest burden of TB, with an estimated incidence figure of 2.1 million cases out of the 9 million cases of TB globally.Diagnosis of TB relies on conventional microscopy and culture with drawbacks related to sensitivity, specificity, turn around time (TAT). The aim ofthis study was to evaluate the performance of Xpert MTB/rifampicin (RIF) assay (GX) for MTB detection in pulmonary and extrapulmonary clinicalsamples.Methods: A total of 209 clinical specimens (182: pulmonary and 27: extrapulmonary) were processed using auramine smear, culture by mycobacteriagrowth indicator tube and GenXpert.Results: The sensitivity of GenXpert was 62.63% for pulmonary and 55% for extrapulmonary samples. The sensitivity and specificity of GX were100% for the smear positive cases. The sensitivity, specificity, positive predictive value, and negative predictive value of the GX for smear negativecases were 67.8%, 97.5%, 90.4%, and 89.6%, respectively. RIF resistance was detected in 3.8% the samples.Conclusion: GenXpert, with short TAT, high sensitivity, specificity and less technical expertise required is a promising tool in TB diagnostics for thefuture.Keywords: GenXpert, Tuberculosis diagnosis, Molecular method, Rifampicin resistance.
COMPARATIVE ANALYSIS OF ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL CONTENTS IN ETHANOLIC LEAF EXTRACTS OF IN VITRO AND FIELD GROWN WITHANIA SOMNIFERA
Authors: Jassal Prabhjot Singh , Kaur Gagandeep
Published: April 2017

ABSTRACTObjective: The present study was planned to compare antioxidant activity in vitro and field grown Withania somnifera was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (NO) assays. Medicinal plants are a major source of phytochemicals used for the treatments ofhuman diseases. W. somnifera has anti-inflammatory, antioxidant, and antimicrobial properties.Methods: Antioxidant activity and phytochemical contents in W. somnifera were determined spectrophotometrically.Results: The results of antioxidant activity of field grown ethanolic leaf extract of W. somnifera showed maximum inhibition of 72.08% and 77.85%in DPPH (50 µg/ml) and NO (100 µg/ml) scavenging assays, respectively. Field grown ethanolic leaf extract of W. somnifera showed maximumconcentrations of phenolics, flavonoids, and carotenoids, as active phytochemicals, determined spectrophotometrically, which were found as676.5 µg/ml, 557.5 µg/ml, and 469 µg/ml, respectively, as compared to in vitro plant extracts.Conclusions: This study demonstrated that antioxidant activity and phytochemical contents of field grown ethanolic leaf extract of W. somnifera werefound to be comparatively higher than in vitro plant extracts. Leaf extracts of W. somnifera are a potential source of antioxidants and could preventmany free radical-related diseases.Keywords: Carotenoids content, 1-diphenyl-2-picrylhydrazyl scavenging assay, Flavonoids content, Nitric oxide radical scavenging assay, Phenoliccontent.
EVALUATION OF THE ANTI-INFLAMMATORY ACTIVITY OF COMBINATION OF ETHANOL EXTRACTS OF AZADIRACHTA INDICA (NEEM) AND LAWSONIA INERMIS (HENNA)
Authors: Rao Tirupathi , P Renuka , P Akhil , P Divya , Priyanka P Devi
Published: April 2017

ABSTRACTObjective: The aim of the present study was to investigate the in vitro anti-inflammatory activity of Azadirachta indica (neem) and Lawsonia inermis(henna) individual extract and in combination using the same solvent.Methods: The leaf material of A. indica and L. inermis was collected from surroundings of Aditya College of Pharmacy, Kakinada, East Godavari.Powdered material was subjected to successive solvent extraction process. The yield was collected and prepared different concentrations (50, 100,and 200 µg/ml) of plant extracts. Diclofenac sodium was used as standard drug. The anti-inflammatory activity was performed by in vitro methodssuch as albumin denaturation method and human red blood cells membrane lysis method.Results: Denaturation of proteins is a well-documented cause of inflammation. Neem showed a significant membrane stabilizing activity of 46.62%and protein denaturation inhibition activity of 57.32% at concentration of 200 µg/ml. Henna showed a significant membrane stabilizing activityof 39.89% and protein denaturation inhibition activity of 53.75% at 200 µg/ml. In combination, both the extracts showed a significant membranestabilizing activity of 56.63% and protein denaturation inhibition activity of 67.69% at concentration of 200 µg/ml.Conclusion: The present study concluded that combination of A. indica and L. inermis possesses significant anti-inflammatory activity when comparedwith individual extract.Keywords: Anti-inflammatory, Human red blood cell, Protein denaturation, Lawsonia inermis, Azadiracta indica.
A COMPARATIVE STUDY OF IN VITRO ANTIMICROBIAL ACTIVITY AND TLC STUDIES OF PETALS OF SELECTED INDIAN MEDICINAL PLANTS
Authors: Rengarajan Sumathy , Melanathuru Vijayalakshmi , Munuswamy Deecaraman , Sundaram Sankaranarayanan , Selvaraj Saravanan Thiruverkadu
Published: April 2017

ABSTRACTObjective: The present study was to evaluate the in vitro antibacterial activity, and thin-layer chromatography (TLC) studies from the petals of fourdifferent Indian medicinal plants (Punica granatum, Hibiscus rosa-sinensis, Cassia auriculata, and Moringa oleifera).Methods: The phytochemical screening of the methanol extract of petals of four different Indian medicinal plants was performed using standardprocedures. The antimicrobial activity was tested against various test organisms using the agar disc diffusion method.Results: The preliminary phytochemical screening for petals of four different medicinal plants revealed the presence of flavonoids, alkaloids, tannins,and saponins. From the above study, the results indicated that the methanol extract of M. oleifera petals showed the highest antimicrobial activityagainst Staphylococcus aureus and Bacillus subtilis with zone of inhibition 17.93 and 23.40, respectively, at the concentration of 20 µl/ml and alsoshowed the maximum inhibitory activity at the highest concentration (20 µl/ml) than the lowest concentration (5 µl/ml) against Gram-negativebacteria such as Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Gram-positive B. subtilis and S. aureus. TLC studies of methanolextracts of petals of Indian medicinal plants revealed the presence of different phytoconstituents as evidenced by separated compounds with differentRf values.Conclusion: The results obtained in the present study indicate that the petals of four different Indian medicinal plants showed the highest antibacterialactivity and can be used as an antibacterial agent against bacterial diseases.Keywords: Phytochemicals, Antibacterial activity, Thin-layer chromatography.
CONTROLLED DELIVERY OF ANTIRETROVIRAL DRUG-LOADED CROSS-LINKED MICROSPHERES BY IONIC GELATION METHOD
Authors: Princely S. , Basha N Saleem , Basha N Saleem , S Nandhakumar , Md Dhanaraju
Published: April 2017

ABSTRACTObjective: Lamivudine (LVD) is a nucleoside reverse transcriptase inhibitor originally developed as an antiretroviral drug and primarily used in thetreatment of most common chronic disease of the planet, acquired immune deficiency syndrome and hepatitis B. The main objective of the study is todevelop controlled drug delivery system to increase the efficacy of antiretroviral drug, LVD against human immunodeficiency virus infections.Methods: The microencapsulation of LVD in gelatin microspheres was carried out by cross-linking process with glutaraldehyde saturated tolueneusing ionic-gelation method. The prepared microspheres were evaluated for particle size analysis, % yield value, % drug content, drug entrapmentefficiency, scanning electron microscopy for surface morphology, swelling index, accelerated stability studies, Fourier transform infrared radiationspectroscopy (FT-IR) and differential scanning calorimetry (DSC) for polymer drug compatibility, in vitro dissolution efficiency and release kineticstudies.Results: The obtained microspheres showed very smooth surface and exhibited regular spherical geometry due to higher crosslinking density. FT-IRand DSC revealed the absence of drug polymer interactions. The percentage yield, entrapment efficiency and drug content for F6 LVD microsphereswas found to be 79.31%, 65.55% and 96.25% respectively. The particle size was ranged from 34.61% to 51.45 µm sizes and in vitro release profileshowed that cross-linking density of gelatin microspheres effectively controlled the release of LVD.Conclusion: The findings of our investigation demonstrated that F6 of gelatin-LVD microspheres had good controlled release profile with maximumentrapment efficiency and prolonged drug release for 24 hrs or longer and this formulation would be capable of overcoming the drawbacks andlimitations of LVD conventional dosage forms.Keywords: Lamivudine, Microspheres, Controlled release, Gelatin, Fourier transform infrared, Differential scanning calorimetry, In vitro releasekinetics.
BIOACTIVE AND FUNCTIONAL PROPERTIES OF FISH PROTEIN HYDROLYSATE FROM LEIOGNATHUS BINDUS
Authors: Johnrose Prabha , Vincent Savariar , Joseph Swapna , Magdalene Joacy
Published: April 2017

ABSTRACTObjective: The present work is focused on the production of biologically active protein hydrolysate from low value by-catch fish, Leiognathus bindus;this will be an ideal approach to exploit the underutilized fish to develop a highly nutritious bioactive product. Moreover, fish protein hydrolysateshave a broad spectrum of applications ranging from high-value peptones to food ingredients with special functional properties.Methods: The protein hydrolysate was prepared from L. bindus through acidic autolysis processes. The proximate composition, physico-functional,and biochemical properties of hydrolysate and control samples were characterized by standard protocols. Molecular profiling was done by sodiumdodecyl sulfate polyacrylamide gel electrophoresis and high-performance liquid chromatography analysis performed to identify the essential aminoacid composition in protein hydrolysate.Results: The results of this study reveals that the protein hydrolysate obtained from the trash fish has relatively high protein (71.01±0.2%) and lowlipid (1.57±0.1%) content. The molecular analysis shows low molecular weight peptides are obtained in the hydrolyzed samples which indicate highprotein solubility confirmed to have the potential application as an ingredient in the balanced human diet. The chemical score of the hydrolysateindicates that it fulfills adult human nutritional requirements.Conclusion: This approach also offers the relatively simple and cost-effective process to improve the nutritional characteristics and functionalproperties of foodstuff, also an opportunity for the conversion of waste fish resources to nutritious products, that can be used in food andpharmaceutical industries.Keywords: Protein hydrolysate, Emulsifying property, Bioactive, Peptides, Amino acid, Chemical score.
EFFECT OF YOHIMBINE ON CLOMIPRAMINE-INDUCED SEXUAL DYSFUNCTION IN MALE RATS
Authors: Shekar Devangam Sheshadri
Published: April 2017

Object: The present investigation has been carried out to find out the effect of yohimbine on clomipramine-induced sexual dysfunction in male rats.Methods: The male rats were treated with clomipramine and yohimbine simultaneously for 60 days. During the treatment, all the male rats werechallenged with the female rats which are in estrous phase and their sexual behavior was observed under dim red light. Half of the animals in each group and remaining on 60 day were sacrificed, blood was collected and serum separated. Testis was collected and preserved in 10% formalin forsubsequent histopathological examination. thResults: The study reveals that yohimbine failed to antagonize the clomipramine-induced sexual dysfunction in male rats in all aspects, except thepartial improvement in the sexual behavior.Conclusion: Yohimbine a well-known aphrodisiac failed to antagonize the clomipramine-induced sexual dysfunction in male rats. The decrease intestosterone levels, a decrease in spermatozoa count were continued even in the presence of yohimbine except improvement in the sexual behaviorparameters. Hence, yohimbine could not be a safe antidote against clomipramine-induced sexual dysfunction in male rats.Keywords: Yohimbine, Clomipramine, Testosterone, Male rat sexual competence, Testicular damage.
PHYTOCONSTITUENTS, PIGMENTS , G.C.M.S. ANALYSIS AND ALLELOPATHY EFFECT OF ALTERNANTHERA FICOIDEA (L.) P. BEAUV
Authors: Patil Reshma Bhagawanrao , Kore Basavaraj A
Published: April 2017

Objective: The aim was to investigate phytoconstituents, composition of pigments, gas chromatography mass spectrometry (GCMS) and allelopathy of Alternanthera ficoidea (L.) P. Beauv.Methods: Qualitative phytochemical analysis was carried out by the method of Paech and Tracey using five different solvent systems (D.W., ether, chloroform, ethanol, and methanol). Methanol leaf extract of this plant was analyzed using GCMS. Chlorophylls, carotenoid, and polyphenols were estimated by following standard methods. Allelopathy effect was studied using leaf leachets on seeds of jowar and mung.Result and Conclusion: Investigation of above parameters in A. ficoidea showed presence of large amount of pharmaceutically important phytochemicals like tannins, saponins, phytols, carotenoids, xanthophylls and polyphenols. It indicates that this weed can be used in herbal medicines and dietary supplements. Since this weed is showing allelopathy effect it should be eradicated from fields before it spreads and occupies the place meant for crop plants.Keywords: Allelopathy, Alternanthera ficoidea, Exotic weed, Phytochemicals.
A STUDY OF DRUG UTILIZATION PATTERN OF THE GERIATRIC PATIENTS IN THE DEPARTMENT OF GERIATRIC MEDICINE IN A TERTIARY CARE HOSPITAL IN ASSAM, INDIA
Authors: Borah Lakhimi , Devi Diptimayee , Debnath Prasanjit Kumar , Deka Dibyajyoti
Published: April 2017

Objective: To investigate the drug utilization pattern and the prevalence of potentially inappropriate medications (PIMs) using updated AGS Beers criteria 2012 among the elderly patients who attended the geriatric outpatient departments as well as admitted  indoor patients ina tertiary care Hospital at Guwahati, Assam, India.Method: A hospital based prospective, observational and cross sectional study, involving patients aged 65 years and above was planned and conducted over a period of 6 months from January 2016 to June 2016. Patients were visited daily, interviewed and case records were collected. Data were analyzed using Microsoft Office Excel Sheets.Results: A total of 150 patients aged 65 years and above were analyzed. 97 patients (65%) were males and 53 (35%) were females. Majority of the patients (61 numbers, 41%) belonged to the age group of 65-70 years, and least were in the age group of 86-90 years (13 numbers, 9%). Prescription of five or more medications (polypharmacy) was observed in 117 (78%) patients. Majority of the patients presented with diseases of cardiovascular system (25.7%). Using WHO drug use indicators, the average number of drugs per prescription was found to be 5.6. The percentage of drugs prescribed by generic name was 86%. The percentage of encounters in which antibiotics were prescribed was  61%. Injection was prescribed was 65% cases and the percentage of drugs prescribed from the NLEM (National List of Essential Medicines of India) was 96%.  43 patients received PIMs from Beers list; majority were belonging to category 1. Spironolactone was most commonly prescribed PIM.Conclusion:  Irrational prescribing practices and polypharmacy were detected in our study.Prescribers should be educated about rational use of drugs and Beers criteria for elderly. Keywords: Beers criteria, WHO prescribing indicators, Potentially inappropriate medications, Elderly patients, Polypharmacy
EFFECTIVITY AND PHYSICOCHEMICAL STABILITY OF NANOSTRUCTURED LIPID CARRIER COENZYME Q10 IN DIFFERENT RATIO of LIPID ALFA CETYL PALMITATE AND ALPHA TOCOPHERYL ACETATE AS CARRIER
Authors: Putranti Astridani Rizky , Hendradi Esti , Primaharinastiti Riesta
Published: April 2017

Objective: The aim of this study was to investigate physical characteristics of nanostructured lipid carriers (NLCs) mixture of alpha tocopheryl acetate, cetyl palmitate, Tween 80 and propylene glycol using high shear homogenization technique on NLC preparation to predict the optimum ratio of alpha tocopheryl acetate-cetyl palmitate to produce good characteristics of NLC loaded coenzyme, higher % EE, good penetration, controlled release, and stable.Methods: Lipid characterizations were conducted by diffraction scanning calorimetry, X-ray diffraction, and Fourier transforms infrared spectrophotometry. Coenzyme Q10 concentration was measured by spectrophotometer at 275 nm. NLC characteristics based on their morphology was determined using transmission electron microscope, particle size, and its polydispersity index which were measured with Delsa Nano™ particle size analyzer. Percentage of coenzyme Q10 entrapped in NLC was determined by dialysis bag method. Coenzyme Q10 release profile was measured using with Franz cell for 12 hrs. The penetration depth of NLC coenzyme Q10 in abdominal skin of Wistar rat was determined with fluorescence microscopy using rhodamine B as marker. NLC physical stability based on minimum of particle size variation, pH and viscosity during 90 days storage.Results: The result showed that formula with ratio of cetyl palmitate-alpha tocopheryl acetate 70:30 (% w/w) produce good characteristics of NLCloaded coenzyme, higher % EE, good penetration, controlled release, and stable in 90 days storage.Conclusion: The coenzyme Q10 NLC system with cetyl palmitate and alpha tocopherol acetate as lipid matrixare characterized by small particle size, low crystallinity, spherical morphology of particle and high coenzyme Q10 entrapment efficiency. Crystal modification led to the formation of a more amorphous thereby increasing the drug entrapmentKeywords: Coenzyme Q10, Nanostructured lipid carrier, Cetyl palmitate, Alpha tocopheryl acetate, High shear homogenization.
THE EFFECTIVENESS OF PRE OPERATIVE DEEP BREATHING EXERCISE ON POST OPERATIVE PATIENTS OF ABDOMINAL SURGERY
Authors: Kale Pratiksha Milind , Mohite Vaishali R , Chendake Mahesh Bhupal , Gholap Manisha C
Published: April 2017

Introduction : Pulmonary complications have a significant impact on morbidity and mortality after major abdominal surgery. One of the major causes of pulmonary dysfunction after such surgery is restricted breathing due to pain and diaphragmatic dysfunction.Methodology :  this study investigated the effects of pre operative breathing exercises training on the vital capacity and peak expiratory flow rate of upper abdominal surgery patients. The patient were divided into 2 groups, control and experimental. Patient in experimental group were given three supervised session of diaphragmatic deep breathing exercise daily. Spirometric and peak flow meter values of vital capacity and peak expiratory flow rate were obtained one day before and 1st , 3rd , 5th, and 7th day after surgery.Result and conclusion : The data obtained were analyzed in terms of descriptive(frequency, percentage, mean and standard deviation) and inferential statistics by using soft ware SPSS – 16.1 version. The analysis showed a significant difference in the pre and post training vital capacity and peak expiratory flow rate for experimental group compared to control group. So, diaphragmatic deep breathing exercise improves the pulmonary functions after the abdominal surgery.
EFFECTIVENESS OF ILIOTIBIAL BAND STRETCHING AND STRENGTHENING EXERCISES IN PATELLOFEMORAL PAIN SYNDROME
Authors: Malarvizhi Devadhason , Bhatt Neha
Published: April 2017

Objective: The study objective was to find out the effectiveness of iliotibial band stretching and strengthening exercises in patellofemoral pain syndrome.Methods: The study design was Quasi-experimental study design. 20 patients with patellofemoral pain syndrome were taken, with the age group of 18-40 years. 20 patients undergone pre- and post-test measurement of hip angle of adduction with the help of inclinometer and were given interferential therapy and iliotibial band stretching and strengthening exercises for 1-week duration. Outcome measures were inclinometer and visual analog scale (VAS).Results: Statistical analysis was done using Paired t-test which showed a significant improvement in VAS score (2.57±1.09) and angle of hip adductionpre- and post-test (−6.85±2.50).Conclusion: This study concluded that 1 week of stretching and strengthening of iliotibial band exercises were effective in reducing pain and increasing hip adduction angle in patellofemoral pain syndrome.Keywords: Iliotibial band, Interferential therapy, Test, Stretching and strengthening exercises, Inclinometer.
INSILICO DISCOVERY OF HUMAN AURORA B KINASE INHIBITORS BY MOLECULAR DOCKING, PHARMACOPHORE VALIDATION AND ADMET STUDIES
Authors: Vadlakonda Rajashekar , Enaganti Sreenivas , Nerella Raghunandan
Published: April 2017

Objectives: To predict the anticancer potentiality of some newly designed azaindole derivatives gainst human Aurora B kinase and to identify the critical features important for their activity.Methods: Initially, the derivatives of azaindoles, (Z)-2-(oxo-1 H-pyrrolo [2,3-b] pyridine-3 (2H)-ylidene)-N-(p-substituted) hydrazine carbothioamide (scaffold A), (E)-3-((E)-substituted benzylidene hydrazono)-1H-pyrrolo[2,3-b]pyridine-2(3H)-one (scaffold B), and 1-(2-substituted acetyl)-1H- pyrrolo [2,3-b]pyridine-2,3-dione are synthesized and sketched using ACD/ChemSketch (12.0). With the 3D converted compounds, docking into the active site of the retrieved protein Aurora B kinase is carried out using LibDock module of discovery studio (DS). Further absorption, distribution, metabolism, excretion and toxicity (ADMET) properties, ligand, and structure-based pharmacophore modeling are applied using DS.Results: Through docking and pharmacophore studies, it is revealed that compound C13 (N-{(Z)-2-[4-(dimethylamino)phenyl]ethenyl}-1H- pyrrolo[2,3-b]pyridine-3-carboxamide) shows the highest binding affinity and good pharmacophoric features with acceptable fit values of both ligand and structure-based pharmacophore models. Furthermore, the calculated ADMET properties are reliable.Conclusion: These studies suggest that the compound C13 (N-{(Z)-2-[4-(dimethylamino)phenyl]ethenyl}-1H-pyrrolo[2,3-b]pyridine-3-carboxamide)may act as a potent target in the anticancer therapy.Keywords: Aneuploidy, Aurora B kinase, Azaindole, Cancer, Cell cycle, Genome stability.