bluuebunny/crossref_metadata_2025 huggingface dataset:
Perceptions of Environmental Influence and Environmental Information-Seeking Behavior Among People With Asthma and COPD
Authors: Parikh Shivani , Henderson Kelly , Gondalia Rahul , Kaye Leanne , Remmelink Esther , Thompson Alesha , Barrett Meredith
Published: May 2022

Environmental exposures and socioeconomic status (SES) are associated with asthma and chronic obstructive pulmonary disease (COPD) morbidity and mortality. Despite efforts to reduce the impact of environmental exposures through regulation and education, knowledge gaps remain. We sought to understand how adults with asthma and COPD perceive and seek information about environmental factors, and how these responses varied by disease or socioeconomic characteristics. Participants with self-reported asthma or COPD enrolled in a digital platform for respiratory disease self-management, consisting of sensors to track medication use and a companion smartphone app, completed an electronic survey exploring perceptions of environmental factors. Using mixed-method analyses, we evaluated differences in responses by disease (asthma vs. COPD), education (≤ vs. > some college), annual household income (< vs. ≥ $50,000), and mean annual residential air pollutant exposure (> vs. ≤80th percentile). Survey responses from 698 participants [500 asthma (72%) and 198 COPD (28%)] were analyzed. A high percentage of participants perceived that environmental factors could influence their symptoms, including: pollen (93% for asthma vs. 86% for COPD), mold (89 vs. 85%), second-hand smoke (89 vs. 83%), and air pollution (84% for both). Participants reported seeking environmental information daily from an average of three sources, preferring mobile apps and television (TV) programs. Significant differences were identified by disease.

Conclusion

Participants with asthma and COPD perceive a relationship between their respiratory symptoms and their environment and regularly seek out environmental information. This information can help inform digital health development for respiratory education and self-management.
Spatiotemporal Clustering of Seismicity in the Kiskatinaw Seismic Monitoring and Mitigation Area
Authors: Khajehdehi Omid , Eaton David W. , Davidsen Jörn
Published: May 2022

Induced seismicity has become a concern for industry and nearby residents. Fluid-induced earthquakes are a side effect of industrial operations such as hydraulic fracturing, where high-pressure fluids are pumped into the Earth’s crust to increase hydrocarbon flow to a well from petroleum-bearing rock formations. Previous studies on fluid-induced seismicity showed the existence of spatiotemporal clustering and aftershock activity. Here, we study seismic catalogs from the Kiskatinaw Seismic Monitoring and Mitigation Area (KSMMA), British Columbia, Canada to investigate spatiotemporal clustering and determine the aftershock activity due to secondary triggering mechanisms such as static and dynamic stress changes arising from preceding seismic events. Our analysis is based on modern tools of statistical seismology such as the Bi-test and triggering analysis. We find significant aftershock triggering and spatiotemporal clustering across the KSMMA but with large spatial variations, which might be indicative of pre-existing stresses and fault systems. In particular, aftershock triggering is characterized by rapidly decaying spatial distributions of aftershocks beyond the mainshock rupture area and by a strong dominance of small but frequent triggers, consistent with that observed for hydraulic fracturing and saltwater disposal elsewhere.
The Association Between Serum Estradiol Levels on hCG Trigger Day and Live Birth Rates in Non-PCOS Patients: A Retrospective Cohort Study
Authors: Xu Xiaoyuan , Yang Aimin , Han Yan , Wang Wei , Hao Guimin , Cui Na
Published: May 2022

Objective

To retrospectively analyze the association of serum estradiol (E2) levels on human chorionic gonadotropin (hCG) trigger day and live birth rates (LBRs) in women undergoing fresh embryo transfer and not exhibiting polycystic ovary syndrome.

Design

Retrospective cohort study.

Methods

Analysis of 13,950 patients who had fresh embryo transfer between December 2013 and December 2019. The main outcome measurement was LBRs. Multivariable regression analysis was performed to investigate associations between E2 levels on the hCG trigger day and LBRs. Stratification analysis was performed to test for effect modification in subgroups. Furthermore, a two-piecewise linear regression model was established to find nonlinear relationships.

Results

Multivariable regression analysis showed a significant association between serum E2 levels on the hCG trigger day and LBRs, adjusting for covariates [relative risk (RR) 1.027, 95% confidence interval (CI) 1.007, 1.049]. Stratification analysis showed that the LBRs were positively associated (RR 1.052, 95% CI 1.004, 1.102) with every 1 ng/ml increase of serum E2 on the hCG trigger day for the subgroup with low antral follicle counts on the trigger day. Specifically, a two-piecewise linear regression model showed that there was a positive association (RR 1.188, 95% CI 1.057, 1.334) between serum E2 and LBR for every increase of 1 ng/ml E2 when the concentration of serum E2 was lower than 2.1 ng/ml. However, there was no significant association (RR 1.002, 95% CI 0.971, 1.032) between E2 levels and LBRs when the concentration of E2 was higher than the 2.1ng/ml inflection point.

Conclusions

Serum E2 levels on the hCG trigger day were segmentally connected with LBRs.
Combined Effects of High-Intensity Aerobic Exercise Training and Ziziphus jujuba Extract on Tissue Nesfatin-1 in Rats
Authors: Ghanbari-Niaki Abbass , Hosseini Fahimeh , Broom David Robert , Tejenjari Bahareh , Rahmati-Ahmadabad Saleh
Published: May 2022

Nesfatin-1 is involved in metabolic/feeding regulation and prevention of cardiovascular disease. Previous studies have shown that exercise and herb supplementation can influence nesfatin-1 concentration. The present study investigated the effects of high-intensity training (HIT) and Ziziphus jujuba (ZJ) extract on tissue nesfatin-1 in rats. Twenty-eight female rats were randomly assigned to one of four groups i.e. 1) Saline-Control (SC), 2) Saline-High Intensity Training (ST), 3) Ziziphus jujuba-Control (ZJC), and 4) Ziziphus jujuba-High Intensity Training (ZJT). Rats performed exercise on a treadmill and/or administered supplements intragastrically for 6 weeks, depending on group category. Seventy-two hours after the last training session, rats were anesthetized. Blood, hypothafi 2lamus tissue, heart and gastrocnemius muscles were sent to the laboratory for analyses. Significantly higher nesfatin-1 gene expression and concentration and ATP concentration were found in trained rat. HIT increased plasma High Density Lipoprotein (HDL) and insulin concentration and reduced plasma Triglyceride (TG) and cortisol. ZJ increased tissue nesftain-1 gene expression and concentration while only increasing heart ATP. The combination of exercise and ZJ showed an additive effect compared to each intervention alone on hypothalamus, heart and gastrocnemius NUCB2 gene expression, heart and gastrocnemius nesfatin-1 concentration, plasma HDL and cortisol concentration. The authors recommend both interventions as a means to improve cardiovascular health in rats with further work needed to confirm similar findings in homo sapiens.
Multi-Parametric Diffusion Tensor Imaging of The Optic Nerve for Detection of Dysthyroid Optic Neuropathy in Patients With Thyroid-Associated Ophthalmopathy
Authors: Liu Ping , Luo Ban , Zhai Lin-han , Wu Hong-Yu , Wang Qiu-Xia , Yuan Gang , Jiang Gui-Hua , Chen Lang , Zhang Jing
Published: May 2022

Objective

To evaluate the microstructural changes of the orbital optic nerve in thyroid-associated ophthalmopathy (TAO) patients with or without dysthyroid optic neuropathy (DON) using diffusion tensor imaging (DTI) and investigate whether DTI can be used to detect DON.

Materials and Methods

59 bilateral TAO patients with (n= 23) and without DON (non-DON, n= 36) who underwent pretreatment DTI were included and 118 orbits were analyzed. The clinical features of all patients were collected. DTI parameters, including mean, axial, and radial diffusivity (MD, AD, and RD, respectively) and fractional anisotropy (FA) of the intra-orbital optic nerve for each orbit were calculated and compared between the DON and non-DON groups. ROC curves were generated to evaluate the diagnostic performance of single or combined DTI parameters. Correlations between DTI parameters and ophthalmological characteristics were analyzed using correlation analysis.

Results

Compared with non-DON, the DON group showed decreased FA and increased MD, RD, and AD (P < 0.01). In the differentiation of DON from non-DON, the MD was optimal individually, and the combination of the four parameters had the best diagnostic performance. There were significant correlations between the optic nerve’s four DTI metrics and the visual acuity and clinical active score (P < 0.05). In addition, optic nerve FA was significantly associated with the amplitude of visual evoked potentials (P = 0.022).

Conclusions

DTI is a promising technique in assessing microstructural changes of optic nerve in patients with DON, and it facilitates differentiation of DON from non-DON eyes in patients with TAO.
Deletion of GPR30 Drives the Activation of Mitochondrial Uncoupling Respiration to Induce Adipose Thermogenesis in Female Mice
Authors: Luo Jing , Wang Yao , Gilbert Elizabeth , Liu Dongmin
Published: May 2022

Thermogenic adipocytes possess a promising approach to combat obesity with its capability promoting energy metabolism. We previously discovered that deletion of GPR30 (GPRKO), a presumably membrane-associated estrogen receptor, protected female mice from developing obesity, glucose intolerance, and insulin resistance when challenged with a high-fat diet (HFD). In vivo, the metabolic phenotype of wild type (WT) and GPRKO female mice were measured weekly. Acute cold tolerance test was performed. Ex vivo, mitochondrial respiration of brown adipose tissue (BAT) was analyzed from diet-induced obese female mice of both genotypes. In vitro, stromal vascular fractions (SVF) were isolated for beige adipocyte differentiation to investigate the role of GPR30 in thermogenic adipocyte. Deletion of GPR30 protects female mice from hypothermia and the mitochondria in BAT are highly energetic in GPRKO animals while the WT mitochondria remain in a relatively quiescent stage. Consistently, GPR30 deficiency enhances beige adipocyte differentiation in white adipose tissue (WAT) and activates the thermogenic browning of subcutaneous WAT due to up-regulation of UCP-1, which thereby protects female mice from HFD-induced obesity. GPR30 is a negative regulator of thermogenesis, which at least partially contributes to the reduced adiposity in the GPRKO female mice. Our findings provide insight into the mechanism by which GPR30 regulates fat metabolism and adiposity in female mice exposed to excess calories, which may be instrumental in the development of new therapeutic strategies for obesity.
Day-Ahead Operation of an Urban Energy System Considering Traffic Flows and Peak Shaving
Authors: Peng Yan , Ma Junchao , Lu Chengyu , Chen Sheng , Pei Lei
Published: May 2022

With the increasing penetration of new-type loads such as electric vehicles and hydrogen fuel vehicles in urban power grids, the peak-to-valley load difference increases sharply, and a multi-energy coordination model is desirable. This article proposes a day-ahead operation model of an urban energy system considering traffic flows and peak shaving, which can positively contribute to multi-energy complement and low-carbon emission. The proposed model minimizes the total cost of electricity and gas by optimizing the charging and discharging strategies of energy storage, in which the output of the wind turbine and energy management of the energy hub are adaptively adjusted. The urban energy system is represented by a second-order cone (SOC) energy flow model, and hence, the optimization problem is modeled as a mixed integer SOC programming (MISOCP). Finally, test results on an integrated urban energy network indicate that the energy storage and multi-energy coordination can alleviate the peak load cutting and valley filling. The relationship between urban grid operation cost and peak-valley difference is also discussed. The maximum utilization of renewable energy sources using gasoline vehicles has been presented in this study to illustrate cost and emission reductions for a sustainable integrated electricity and transportation infrastructure.
Comparative Spatial Assessment of Trace Metal(loid) Pollution in the Sediments of the Lower Olifants River Basin in South Africa
Authors: Addo-Bediako Abraham
Published: May 2022

Globally, many freshwater ecosystems are facing chemical pollution from both natural and anthropogenic sources. The Olifants River Basin in South Africa is experiencing degradation due to mining, industrial, agricultural, and domestic activities. The impacts of these activities coupled with climate change is likely to shift the hydrological cycle that may increase accumulation of toxic chemicals in the sediments. The aim of the study was to assess the contamination of As, Cr, Cu, Fe, Mn, Ni, Pb, and Zn in the sediments collected at the upstream, midstream and downstream of four rivers of the Lower Olifants River Basin; the Blyde, Mohalpitsi, Ga-Selati, and Steelpoort rivers. The highest concentrations of most of the trace metal (loid)s assessed were from the Steelpoort River followed by the Blyde River. Significant diﬀerences in metal concentrations were found across study sites and rivers. The overall assessment of the sub-catchment, which is characterised mainly by mining and agricultural activities shows significantly elevated levels of As, Cr, Mn, and Ni in the sediments and may cause secondary pollution in the water. Using enrichment factor (EF) and geo-accumulation index (Igeo), some of the sediments were severely enriched and extremely contaminated respectively with As, Cr, and Ni. This may risk the lives of aquatic biota and humans, especially rural communities that rely on these rivers for drinking water. The findings provide baseline information for effective management control of metal(loid) pollution in the Olifants River Basin.
The Role of MicroRNA in DNA Damage Response
Authors: Li Yongxin , Tong Yan , Liu Jiaqi , Lou Jianlin
Published: May 2022

DNA is essential for the development and function of organisms. A number of factors affect DNA integrity and cause DNA damages, such as ultraviolet light, ionizing radiation and hydrogen peroxide. DNA damages activate a series of intracellular reactions, called DNA damage response, which play a crucial role in the pathogenesis of cancers and other diseases. MiRNA is a type of evolutionarily conserved non-coding RNA and affects the expression of target genes by post-transcriptional regulation. Increasing evidences suggested that the expression of some miRNAs was changed in tumor cases. MiRNAs may participate in DNA damage response and affect genomic stability via influencing the processes of cell cycle, DNA damage repair and apoptosis, thus ultimately impact on tumorigenesis. Therefore, the role of miRNA in DNA damage response is reviewed, to provide a theoretical basis for the mechanism of miRNAs’ effects on DNA damage response and for the research of new therapies for diseases.
Prognostic Biomarker KIF18A and Its Correlations With Immune Infiltrates and Mitosis in Glioma
Authors: Tao Bing-Yan , Liu Yu-Yang , Liu Hong-Yu , Zhang Ze-Han , Guan Yun-Qian , Wang Hui , Shi Ying , Zhang Jun
Published: May 2022

Background: Glioma is globally recognised as one of the most frequently occurring primary malignant brain tumours, making the identification of glioma biomarkers critically significant. The protein KIF18A (Kinesin Family Member 18A) is a member of the kinesin superfamily of microtubule-associated molecular motors and has been shown to participate in cell cycle and mitotic metaphase and anaphase. This is the first investigation into the expression of KIF18A and its prognostic value, potential biological functions, and effects on the immune system and mitosis in glioma patients.

Methods: Gene expression and clinicopathological analysis, enrichment analysis, and immune infiltration analysis were based on data obtained from The Cancer Genome Atlas (TCGA), with additional bioinformatics analyses performed. Statistical analysis was conducted in R software. Clinical samples were used to evaluate the expression of KIF18A via immunohistochemical staining. In addition, the expression level of KIF18A was validated on U87 cell line.

Results: Our results highlighted that KIF18A plays a key role as an independent prognostic factor in patients with glioma. KIF18A was highly expressed in glioma tissues, and KIF18A expression was associated with age, World Health Organization grade, isocitrate dehydrogenase (IDH) status, 1p/19q codeletion, primary therapy outcome, and overall survival (OS). Enrichment analysis revealed that KIF18A is closely correlated with the cell cycle and mitosis. Single sample gene set enrichment analysis (ssGSEA) analysis revealed that KIF18A expression was related to the immune microenvironment. The increased expression of KIF18A in glioma was verified in clinical samples and U87 cell line.

Conclusion: The identification of KIF18A as a new biomarker for glioma could help elucidate how changes in the glioma cell and immune microenvironment promote glioma malignancy. With further analysis, KIF18A may serve as an independent prognostic indicator for human glioma.
Chronic Kidney Failure Provokes the Enrichment of Terminally Differentiated CD8+ T Cells, Impairing Cytotoxic Mechanisms After Kidney Transplantation
Authors: Leonhard Jonas , Schaier Matthias , Kälble Florian , Eckstein Volker , Zeier Martin , Steinborn Andrea
Published: May 2022

Chronic kidney failure (KF) provokes the development of immune senescent CD8+ cytotoxic T cells, affecting the occurrence of graft rejection, viral infections, and malignancies after kidney transplantation. In this study, we analyzed the impact of KF, subsequent dialysis treatment, and kidney transplantation on the differentiation of CD8+CD31+CD45RA+CCR7+ recent thymic emigrant (CCR7+ RTE) Tregs/Tresps into CD8+CD31-CD45RA- memory (CD31- memory) Tregs/Tresps and its effect on the release of cytokines, Fas receptor, Fas ligand as well as cytotoxic mediators by naïve, central memory (CM), effector memory (EM), and terminally differentiated effector memory (TEMRA) Tresps. We found that normal age-dependent differentiation of CD8+ Tregs/Tresps generally differs in the way that TEMRA cells only arise in Tresps. Compared to healthy controls, KF patients revealed an age-independently decreased frequency of CCR7+ RTE Tregs/Tresps, but increased frequencies of CCR7+ MN Tregs/Tresps and CD31- memory Tregs/Tresps, suggesting an increased differentiation via CD31+CD45RA- memory (CD31+ memory) Tregs/Tresps into CD31- memory Tregs/Tresps. Intensified differentiation via CD31+ memory Tresps increased the emergence of apoptosis-resistant CM Tresps with strong Fas ligand-mediated cytotoxicity. CCR7+ RTE Tresp proliferation generated TEMRA Tresps, secreting high levels of cytotoxic mediators. In dialysis and transplant patients, CD31+ TEMRA Tregs/Tresps accumulated, proposing an impaired CCR7+ RTE Treg/Tresp differentiation via CD31+ memory Tregs/Tresps into CD31- memory Tregs/Tresps. Increased percentages of CD31- TEMRA Tresps, but not of CD31- TEMRA Tregs, were observed in all patient groups, indicating impaired proliferation of CCR7+ RTE Tresps, but not of CCR7+ RTE Tregs, into CD31- memory Tregs/Tresps. In transplant patients, CCR7+ RTE Tregs accumulated, while frequencies of CCR7+ RTE Tresps were decreased, suggesting that the immunosuppressive therapy only prevented excessive CCR7+ RTE Treg differentiation but not that of CCR7+ RTE Tresps. Presumably, this caused the accumulation of TEMRA Tresps with decreased release of cytotoxic mediators, such as perforin. In conclusion, we propose that chronic KF affects both the differentiation of CD8+ Tregs and CD8+ Tresps. However, the immunosuppressive therapy after transplantation may successfully prevent excessive Treg differentiation, but not as suffciently that of Tresps. Therefore, the risk for graft rejection may be reduced, while the susceptibility for infections and malignancies may be increased in these patients.
Association Between Endometriosis and Subsequent Risk of Sjögren’s Syndrome: A Nationwide Population-Based Cohort Study
Authors: Chao Yung-Hsiang , Liu Chin-Hsiu , Pan Yu-An , Yen Fu-Shun , Chiou Jeng-Yuan , Wei James Cheng-Chung
Published: May 2022

Objective

The relationship between endometriosis and the ensuing risk of Sjögren’s syndrome has remained unclear. This study aims to present epidemiological evidence for this connection.

Methods

This is a retrospective cohort study of endometriosis patients (ICD-9-CM 617.0-617.9 and 621.3) and matched comparison group between 2000 and 2012 in the National Taiwan Insurance Research Database. After age matching, we analyzed the association between endometriosis and Sjögren’s syndrome (ICD-9-CM 710.2). We used the Cox proportional hazard model to examine the hazard ratio of incidental Sjögren’s syndrome. Subgroup analyses on age, comorbidities, and disease duration were also performed.

Results

A total of 73,665 individuals were included in this study. We identified 14733 newly diagnosed endometriosis patients and 58,932 non-endometriosis comparison group. The adjusted hazard ratio (HR) for incidental Sjögren’s syndrome was 1.45 (95% confidence interval CI=1.27-1.65) in the endometriosis group, compared to the non-endometriosis comparison group. In subgroup analysis, the adjusted HR was 1.53 (95% CI=1.25-1.88) in the age group of 20-39 and 1.41 (95% CI =1.18-1.68) in the age of 40-64. Time-vary analysis showed that endometriosis who have a follow-up time of fewer than five years (adjusted HR=1.57, 95% CI=1.32-1.87) have a significantly highest risk of having subsequent Sjögren’s syndrome.

Conclusion

This population-based cohort study indicated that having a history of endometriosis puts patients at an increased risk of getting Sjögren’s syndrome afterward, especially in the age group of 20-39 and within the first five years after the diagnosis of endometriosis. Clinicians should recognize this possible association in managing endometriosis or Sjögren’s syndrome patients.
The Road to Elimination: Current State of Schistosomiasis Research and Progress Towards the End Game
Authors: Ogongo Paul , Nyakundi Ruth K. , Chege Gerald K. , Ochola Lucy
Published: May 2022

The new WHO Roadmap for Neglected Tropical Diseases targets the global elimination of schistosomiasis as a public health problem. To date, control strategies have focused on effective diagnostics, mass drug administration, complementary and integrative public health interventions. Non-mammalian intermediate hosts and other vertebrates promote transmission of schistosomiasis and have been utilized as experimental model systems. Experimental animal models that recapitulate schistosomiasis immunology, disease progression, and pathology observed in humans are important in testing and validation of control interventions. We discuss the pivotal value of these models in contributing to elimination of schistosomiasis. Treatment of schistosomiasis relies heavily on mass drug administration of praziquantel whose efficacy is comprised due to re-infections and experimental systems have revealed the inability to kill juvenile schistosomes. In terms of diagnosis, nonhuman primate models have demonstrated the low sensitivity of the gold standard Kato Katz smear technique. Antibody assays are valuable tools for evaluating efficacy of candidate vaccines, and sera from graded infection experiments are useful for evaluating diagnostic sensitivity of different targets. Lastly, the presence of Schistosomes can compromise the efficacy of vaccines to other infectious diseases and its elimination will benefit control programs of the other diseases. As the focus moves towards schistosomiasis elimination, it will be critical to integrate treatment, diagnostics, novel research tools such as sequencing, improved understanding of disease pathogenesis and utilization of experimental models to assist with evaluating performance of new approaches.
Intranasal Delivery of Thermostable Subunit Vaccine for Cross-Reactive Mucosal and Systemic Antibody Responses Against SARS-CoV-2
Authors: Nguyen Khue G. , Mantooth Siena M. , Vrabel Maura R. , Zaharoff David A.
Published: May 2022

Despite the remarkable efficacy of currently approved COVID-19 vaccines, there are several opportunities for continued vaccine development against SARS-CoV-2 and future lethal respiratory viruses. In particular, restricted vaccine access and hesitancy have limited immunization rates. In addition, current vaccines are unable to prevent breakthrough infections, leading to prolonged virus circulation. To improve access, a subunit vaccine with enhanced thermostability was designed to eliminate the need for an ultra-cold chain. The exclusion of infectious and genetic materials from this vaccine may also help reduce vaccine hesitancy. In an effort to prevent breakthrough infections, intranasal immunization to induce mucosal immunity was explored. A prototype vaccine comprised of receptor-binding domain (RBD) polypeptides formulated with additional immunoadjuvants in a chitosan (CS) solution induced high levels of RBD-specific antibodies in laboratory mice after 1 or 2 immunizations. Antibody responses were durable with high titers persisting for at least five months following subcutaneous vaccination. Serum anti-RBD antibodies contained both IgG1 and IgG2a isotypes suggesting that the vaccine induced a mixed Th1/Th2 response. RBD vaccination without CS formulation resulted in minimal anti-RBD responses. The addition of CpG oligonucleotides to the CS plus RBD vaccine formulation increased antibody titers more effectively than interleukin-12 (IL-12). Importantly, generated antibodies were cross-reactive against RBD mutants associated with SARS-CoV-2 variants of concern, including alpha, beta and delta variants, and inhibited binding of RBD to its cognate receptor angiotensin converting enzyme 2 (ACE2). With respect to stability, vaccines did not lose activity when stored at either room temperature (21-22°C) or 4°C for at least one month. When delivered intranasally, vaccines induced RBD-specific mucosal IgA antibodies, which may protect against breakthrough infections in the upper respiratory tract. Altogether, data indicate that the designed vaccine platform is versatile, adaptable and capable of overcoming key constraints of current COVID-19 vaccines.
Susceptibility of Caribbean Brain Coral Recruits to Stony Coral Tissue Loss Disease (SCTLD)
Authors: Williamson Olivia M. , Dennison Caroline E. , O’Neil Keri L. , Baker Andrew C.
Published: May 2022

Stony coral tissue loss disease (SCTLD) has devastated coral populations along Florida’s Coral Reef and beyond. Although widespread infection and mortality of adult colonies have been documented, no studies have yet investigated the susceptibility of recruits to this disease. Here, we subjected eight-month-old Diploria labyrinthiformis recruits and four-month-old Colpophyllia natans recruits to two sequential exposures to SCTLD in the laboratory to track infection and assess potential resilience. Both species began to develop lesions as early as 48 h after exposure began. During the first exposure, 59.6% of C. natans recruits lost all tissue (died) within two to eight days of developing lesions, whereas D. labyrinthiformis recruits experienced slower tissue loss and minimal eventual mortality. In C. natans, larger recruits and those fused into groups of multiple genets (chimeras) exhibited the highest survivorship. In contrast, smaller and/or single (ungrouped) recruits had the lowest survivorship (9.9 - 24.8%). After 20 days, a second SCTLD exposure was delivered to further test resistance in remaining recruits, and all recruits of both species succumbed within six days. Although no recruits showed absolute resistance to SCTLD following repeated exposures, our results provide evidence that interactions between size and chimerism can impact relative resistance in C. natans. This study represents the first report of SCTLD in Caribbean coral recruits and carries implications for natural species recovery and reef restoration efforts. Additional research on the susceptibility of coral juveniles to SCTLD is urgently needed, to include different species, locations, parents, and algal symbionts, with the goals of assessing relative susceptibility and identifying potential sources of resilience for this critical life history stage.
Quasi-Synchronous Accumulation of Apparent Oxygen Utilization and Inorganic Carbon in the South Yellow Sea Cold Water Mass From Spring to Autumn: The Acidification Effect and Roles of Community Metabolic Processes, Water Mixing, and Spring Thermal State
Authors: Yu Si-qing , Xiong Tian-qi , Zhai Wei-dong
Published: May 2022

To better understand seasonal acidification in the South Yellow Sea (SYS), four field surveys conducted in 2019 and the historical data obtained in 2018 were incorporated in this study. The lowest aragonite saturation state (Ωarag) value of 1.15 was observed in the central SYS in late autumn. Despite interannual variations in the rate of net community respiration, the quasi-synchronous accumulation of apparent oxygen utilization and excess dissolved inorganic carbon (DIC) relative to the air equilibrium were revealed in the SYS cold water mass (SYSCWM) from late spring to autumn. Correspondingly, the two acidification indexes (Ωarag and pH) decreased in logarithmic forms in the SYSCWM in warm seasons. To examine the potential influences of hydrological dynamics on seasonal acidification in the SYSCWM, a three-endmember water-mixing model was applied. The results showed that the cumulative effect of various non-conservative processes on DIC was comparable with the excess DIC relative to the air equilibrium. This implied that the summer and autumn carbonate dynamics and the acidification status of the cold water mass were almost free from the potential impacts of the weak water mixing and internal circulation in summer and autumn in a given year. The Yellow Sea Warm Current carries oceanic DIC into the SYS only in winter and early spring. This study also showed that the re-equilibrium with atmospheric CO2 at given temperature in early spring determined the initial Ωarag of the SYS before Ωarag declining in late spring, summer, and autumn. The sensitivity of coastal Ωarag changes to DIC addition is subject to both spatial and temporal variations.
A Multi-Modal Energy Harvesting Device for Multi-Directional and Low-Frequency Wave Energy
Authors: Zhu Yikai , Yang Zhou , Jiao Chong , Ma Mengchao , Zhong Xiang
Published: May 2022

As a kind of sustainable energy source, ocean wave energy has always attracted attention. Because of the characteristics of multi-directions and low-bandwidth, the harvesting of wave energy has always been difficult. To harvest broadband wave energy in multiple directions and improve power generation efficiency, we present a multi-modal energy harvesting device by using a cross beam structure. In this device, the efficient harvesting of multi-frequency vibration energy in multiple directions has been achieved by using the multi-modal characteristics of the structure and the high power generation efficiency of the electric device based on liquid metal. Contrast experiments in multiple directions show that the device not only has the capability of multi-directional energy harvesting but also can work in the sweep range of the full band. Under horizontal excitation, compared with traditional single cantilever structure, the peak power density increased to 2227% and the working frequency band increased to 6.25 times. The experimental results show that the device significantly improves the efficiency of low-bandwidth multi-directional energy harvesting, providing a new method for vibration energy harvesting.
Cardiovascular Risk in Myositis Patients Compared to the General Population: Preliminary Data From a Single-Center Cross-Sectional Study
Authors: Oreska Sabina , Storkanova Hana , Kudlicka Jaroslav , Tuka Vladimir , Mikes Ondrej , Krupickova Zdislava , Satny Martin , Chytilova Eva , Kvasnicka Jan , Spiritovic Maja , Hermankova Barbora , Cesak Petr , Rybar Marian , Pavelka Karel , Senolt Ladislav , Mann Herman , Vencovsky Jiri , Vrablik Michal , Tomcik Michal
Published: May 2022

Background

Idiopathic inflammatory myopathies (IIM) are associated with systemic inflammation, limited mobility, and glucocorticoid therapy, all of which can lead to metabolism disturbances, atherogenesis, and increased cardiovascular (CV) risk. The aim of this study was to assess the CV risk in IIM patients and healthy controls (HC), and its association with disease-specific features.

Methods

Thirty nine patients with IIM (32 females; mean age 56; mean disease duration 4.8 years; dermatomyositis: n = 16, polymyositis: n = 7, immune-mediated necrotizing myopathy: n = 8, anti-synthetase syndrome: n = 8) and 39 age-/sex-matched HC (32 females, mean age 56) without rheumatic diseases were included. In both groups, subjects with a history of CV disease (angina pectoris, myocardial infarction, cerebrovascular, and peripheral arterial vascular events) were excluded. Muscle involvement, disease activity, and tissue damage were evaluated (Manual Muscle Test-8, Myositis Intention to Treat Activity Index, Myositis Damage Index). Comorbidities and current treatment were recorded. All participants underwent examinations of carotid intima-media thickness (CIMT), pulse wave velocity (PWV), ankle-brachial index (ABI), and body composition (by densitometry and bioelectric impedance). The risk of fatal CV events was evaluated by the Systematic COronary Risk Evaluation (SCORE, charts for the European population) and its modifications.

Results

Compared to HC, there was no significant difference in IIM patients regarding blood pressure, ABI, PWV, CIMT, and the risk of fatal CV events by SCORE or SCORE2, or subclinical atherosclerosis (CIMT, carotid plaques, ABI, and PWV). The calculated CV risk scores by SCORE, SCORE2, and SCORE multiplied by the coefficient 1.5 (mSCORE) were reclassified according to the results of carotid plaque presence and CIMT; however, none of them was demonstrated to be significantly more accurate. Other significant predictors of CV risk in IIM patients included age, disease duration and activity, systemic inflammation, lipid profile, lean body mass, and blood pressure.

Conclusions

No significant differences in CV risk factors between our IIM patients and HC were observed. However, in IIM, CV risk was associated with age, disease duration, duration of glucocorticoid therapy, lipid profile, and body composition. None of the currently available scoring tools (SCORE, SCORE2, mSCORE) used in this study seems more accurate in estimating CV risk in IIM.
Telehealth Technology Application in Enhancing Continuous Positive Airway Pressure Adherence in Obstructive Sleep Apnea Patients: A Review of Current Evidence
Authors: Thong Benjamin Ka Seng , Loh Grace Xin Yun , Lim Jia Jan , Lee Christina Jia Liang , Ting Shu Ning , Li Hong Peng , Li Qing Yun
Published: May 2022

Obstructive sleep apnea (OSA) is a common type of sleep-disordered breathing associated with multiple comorbidities. Continuous positive airway pressure (CPAP) is the first choice for moderate-severe OSA but poor compliance brings a great challenge to its effectiveness. Telehealth interventions ease the follow-up process and allow healthcare facilities to provide consistent care. Fifth-generation wireless transmission technology has also greatly rationalized the wide use of telemedicine. Herein, we review the efficacy of the telehealth system in enhancing CPAP adherence. We recommend applying telemonitoring in clinical practice and advocate the development of a biopsychosocial telemedicine model with the integration of several interventions. Big databases and promising artificial intelligent technologies make clinical decision support systems and predictive models based on these databases possible.
Unique Geothermal Chemistry Shapes Microbial Communities on Mt. Erebus, Antarctica
Authors: Noell Stephen E. , Baptista Mafalda S. , Smith Emily , McDonald Ian R. , Lee Charles K. , Stott Matthew B. , Amend Jan P. , Cary S. Craig
Published: May 2022

Mt. Erebus, Antarctica, is the world’s southernmost active volcano and is unique in its isolation from other major active volcanic systems and its distinctive geothermal systems. Using 16S rRNA gene amplicon sequencing and physicochemical analyses, we compared samples collected at two contrasting high-temperature (50°C–65°C) sites on Mt. Erebus: Tramway Ridge, a weather-protected high biomass site, and Western Crater, an extremely exposed low biomass site. Samples were collected along three thermal gradients, one from Western Crater and two within Tramway Ridge, which allowed an examination of the heterogeneity present at Tramway Ridge. We found distinct soil compositions between the two sites, and to a lesser extent within Tramway Ridge, correlated with disparate microbial communities. Notably, pH, not temperature, showed the strongest correlation with these differences. The abundance profiles of several microbial groups were different between the two sites; class Nitrososphaeria amplicon sequence variants (ASVs) dominated the community profiles at Tramway Ridge, whereas Acidobacteriotal ASVs were only found at Western Crater. A co-occurrence network, paired with physicochemical analyses, allowed for finer scale analysis of parameters correlated with differential abundance profiles, with various parameters (total carbon, total nitrogen, soil moisture, soil conductivity, sulfur, phosphorous, and iron) showing significant correlations. ASVs assigned to Chloroflexi classes Ktedonobacteria and Chloroflexia were detected at both sites. Based on the known metabolic capabilities of previously studied members of these groups, we predict that chemolithotrophy is a common strategy in this system. These analyses highlight the importance of conducting broader-scale metagenomics and cultivation efforts at Mt. Erebus to better understand this unique environment.
Disordered–Ordered Protein Binary Classification by Circular Dichroism Spectroscopy
Authors: Micsonai András , Moussong Éva , Murvai Nikoletta , Tantos Ágnes , Tőke Orsolya , Réfrégiers Matthieu , Wien Frank , Kardos József
Published: May 2022

Intrinsically disordered proteins lack a stable tertiary structure and form dynamic conformational ensembles due to their characteristic physicochemical properties and amino acid composition. They are abundant in nature and responsible for a large variety of cellular functions. While numerous bioinformatics tools have been developed for in silico disorder prediction in the last decades, there is a need for experimental methods to verify the disordered state. CD spectroscopy is widely used for protein secondary structure analysis. It is usable in a wide concentration range under various buffer conditions. Even without providing high-resolution information, it is especially useful when NMR, X-ray, or other techniques are problematic or one simply needs a fast technique to verify the structure of proteins. Here, we propose an automatized binary disorder–order classification method by analyzing far-UV CD spectroscopy data. The method needs CD data at only three wavelength points, making high-throughput data collection possible. The mathematical analysis applies the k-nearest neighbor algorithm with cosine distance function, which is independent of the spectral amplitude and thus free of concentration determination errors. Moreover, the method can be used even for strong absorbing samples, such as the case of crowded environmental conditions, if the spectrum can be recorded down to the wavelength of 212 nm. We believe the classification method will be useful in identifying disorder and will also facilitate the growth of experimental data in IDP databases. The method is implemented on a webserver and freely available for academic users.
Exercise for Osteoarthritis: A Literature Review of Pathology and Mechanism
Authors: Kong Hui , Wang Xue-Qiang , Zhang Xin-An
Published: May 2022

Osteoarthritis (OA) has a very high incidence worldwide and has become a very common joint disease in the elderly. Currently, the treatment methods for OA include surgery, drug therapy, and exercise therapy. In recent years, the treatment of certain diseases by exercise has received increasing research and attention. Proper exercise can improve the physiological function of various organs of the body. At present, the treatment of OA is usually symptomatic. Limited methods are available for the treatment of OA according to its pathogenesis, and effective intervention has not been developed to slow down the progress of OA from the molecular level. Only by clarifying the mechanism of exercise treatment of OA and the influence of different exercise intensities on OA patients can we choose the appropriate exercise prescription to prevent and treat OA. This review mainly expounds the mechanism that exercise alleviates the pathological changes of OA by affecting the degradation of the ECM, apoptosis, inflammatory response, autophagy, and changes of ncRNA, and summarizes the effects of different exercise types on OA patients. Finally, it is found that different exercise types, exercise intensity, exercise time and exercise frequency have different effects on OA patients. At the same time, suitable exercise prescriptions are recommended for OA patients.
Machine Learning Decomposition of the Anatomy of Neuropsychological Deficit in Alzheimer’s Disease and Mild Cognitive Impairment
Authors: Dong Ningxin , Fu Changyong , Li Renren , Zhang Wei , Liu Meng , Xiao Weixin , Taylor Hugh M. , Nicholas Peter J. , Tanglay Onur , Young Isabella M. , Osipowicz Karol Z. , Sughrue Michael E. , Doyen Stephane P. , Li Yunxia
Published: May 2022

Objective

Alzheimer’s Disease (AD) is a progressive condition characterized by cognitive decline. AD is often preceded by mild cognitive impairment (MCI), though the diagnosis of both conditions remains a challenge. Early diagnosis of AD, and prediction of MCI progression require data-driven approaches to improve patient selection for treatment. We used a machine learning tool to predict performance in neuropsychological tests in AD and MCI based on functional connectivity using a whole-brain connectome, in an attempt to identify network substrates of cognitive deficits in AD.

Methods

Neuropsychological tests, baseline anatomical T1 magnetic resonance imaging (MRI), resting-state functional MRI, and diffusion weighted imaging scans were obtained from 149 MCI, and 85 AD patients; and 140 cognitively unimpaired geriatric participants. A novel machine learning tool, Hollow Tree Super (HoTS) was utilized to extract feature importance from each machine learning model to identify brain regions that were associated with deficit and absence of deficit for 11 neuropsychological tests.

Results

11 models attained an area under the receiver operating curve (AUC-ROC) greater than 0.65, while five models had an AUC-ROC ≥ 0.7. 20 parcels of the Human Connectome Project Multimodal Parcelation Atlas matched to poor performance in at least two neuropsychological tests, while 14 parcels were associated with good performance in at least two tests. At a network level, most parcels predictive of both presence and absence of deficit were affiliated with the Central Executive Network, Default Mode Network, and the Sensorimotor Networks. Segregating predictors by the cognitive domain associated with each test revealed areas of coherent overlap between cognitive domains, with the parcels providing possible markers to screen for cognitive impairment.

Conclusion

Approaches such as ours which incorporate whole-brain functional connectivity and harness feature importance in machine learning models may aid in identifying diagnostic and therapeutic targets in AD.
Skeletal Muscle Involvement Pattern of Hereditary Transthyretin Amyloidosis: A Study Based on Muscle MRI
Authors: Chu Xujun , Du Kang , Tang Yuwei , Zhao Xutong , Yu Meng , Zheng Yiming , Deng Jianwen , Lv He , Zhang Wei , Wang Zhaoxia , Yuan Yun , Meng Lingchao
Published: May 2022

Objects

This study was intended to explore the characteristics of muscle magnetic resonance imaging (MRI) of patients with hereditary transthyretin amyloidosis (ATTRv amyloidosis) prospectively.

Methods

The clinical data of 20 patients with ATTRv amyloidosis at our hospital between July 2020 and August 2021 were analyzed. MRI of lower limbs including calf muscles was performed in all these 20 patients and MRI of thigh muscles was performed in 16 of them.

Results

The mean age of the 20 patients with ATTRv amyloidosis was 44.2 years (ranging from 26 to 60) whose mean duration of weakness was 23.3 ± 23.0 (ranging from 0 to 84) months. All the patients presented with polyneuropathy, and 18 of them with weakness in their lower limbs. Muscle involvement was selective in these patients with ATTRv amyloidosis. The posterior group of muscles was heavily fatty, and the soleus muscle was the most heavily involved. The proportion of fatty infiltration scores at the calf level was higher than at the thigh level with paired comparison for most patients. Three of these patients had more severely fatty infiltration of muscles at the thigh level. The fatty infiltration of posterior compartments at the calf level was highly consistent with neuropathy impairment scores of lower limbs (weakness), the strength of ankle plantar flexion muscles, and the amplitude of the compound muscle action potential of the tibial nerve.

Conclusions

It was found that the pattern of muscle fatty infiltration was consistent with a distal-to-proximal gradient on the whole and that proximal involvements in MRI of lower limbs in some patients could also be observed. Selective fatty infiltration of muscles of posterior compartments and fatty infiltration of the soleus muscle might be typical of ATTRv amyloidosis.