bluuebunny/crossref_metadata_2025 huggingface dataset:
Transformative skeletal motion analysis: optimization of exercise training and injury prevention through graph neural networks
Authors: Zhu Jiaju , Ye Zijun , Ren Meixue , Ma Guodong
Published: March 2024

Introduction

Exercise is pivotal for maintaining physical health in contemporary society. However, improper postures and movements during exercise can result in sports injuries, underscoring the significance of skeletal motion analysis. This research aims to leverage advanced technologies such as Transformer, Graph Neural Networks (GNNs), and Generative Adversarial Networks (GANs) to optimize sports training and mitigate the risk of injuries.

Methods

The study begins by employing a Transformer network to model skeletal motion sequences, facilitating the capture of global correlation information. Subsequently, a Graph Neural Network is utilized to delve into local motion features, enabling a deeper understanding of joint relationships. To enhance the model's robustness and adaptability, a Generative Adversarial Network is introduced, utilizing adversarial training to generate more realistic and diverse motion sequences.

Results

In the experimental phase, skeletal motion datasets from various cohorts, including professional athletes and fitness enthusiasts, are utilized for validation. Comparative analysis against traditional methods demonstrates significant enhancements in specificity, accuracy, recall, and F1-score. Notably, specificity increases by ~5%, accuracy reaches around 90%, recall improves to around 91%, and the F1-score exceeds 89%.

Discussion

The proposed skeletal motion analysis method, leveraging Transformer and Graph Neural Networks, proves successful in optimizing exercise training and preventing injuries. By effectively amalgamating global and local information and integrating Generative Adversarial Networks, the method excels in capturing motion features and enhancing precision and adaptability. Future research endeavors will focus on further advancing this methodology to provide more robust technological support for healthy exercise practices.
Ultra-processed food consumption and chronic kidney disease risk: a systematic review and dose–response meta-analysis
Authors: He Xingzhen , Zhang Xiaoyan , Si Caijuan , Feng Yuliang , Zhu Qin , Li Songtao , Shu Long
Published: March 2024

Background

High intake of ultra-processed food (UPF) has been associated with increased risk of chronic kidney disease(CKD), but the results remain inconsistent. We therefore performed this systematic review and dose–response meta-analysis of observational studies that shed light on the association between UPF consumption and the risk of CKD.

Methods

A systematic literature search of PubMed, Embase, Web of Science, Scopus and China National Knowledge Infrastructure (CNKI) databases was carried out to find the eligible articles published up to October 31, 2023. Random-effects or fixed-effects models were used to pool the relative risks(RRs) and their 95% confidence intervals (CIs).The potential sources of heterogeneity across studies were examined using the Cochran’s Q test and I-square(I2). Publication bias was examined using the visual inspection of asymmetry in funnel plots and quantified by Begg’s and Egger’s tests.

Results

Eight studies (six cohort and two cross-sectional studies) exploring the association between UPF consumption and risk of CKD, were included in the final analysis. The pooled analyses revealed that high consumption of UPF was associated with an increased risk of CKD (RR = 1.25; 95%CI: 1.09–1.42, p < 0.0001). Moreover, a 10% increase of UPF consumption was associated with a 7% higher risk of CKD (RR = 1.07; 95%CI: 1.04–1.10, p < 0.001). Dose–response analysis of all included studies showed a linear association between UPF consumption and the risk of CKD (RR = 1.02; 95%CI:0.99–1.05, Pdose–response = 0.178, Pnonlinearity = 0.843).

Conclusion

Our findings indicate that high consumption of UPF is significantly associated with an increased risk of CKD. Future research with prospective design is required to confirm this positive association.

Systematic review registration: https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023478483, PROSPERO identifier CRD42023478483.
Corn straw-saccharification fiber improved the reproductive performance of sows in the late gestation and lactation via lipid metabolism
Authors: Liu Mengjie , Liu Chaoqi , Shi Jiajia , Wang Ping , Chang Juan , Xu Xiaoxiang , Wang Lijun , Jin Sanjun , Li Xinxin , Yin Qingqiang , Zhu Qun , Dang Xiaowei , Lu Fushan
Published: March 2024

With the development of animal husbandry, the shortage of animal feedstuffs has become serious. Dietary fiber plays a crucial role in regulating animal health and production performance. The aim of this study was to investigate the effects of three kinds of corn straw-saccharification fibers (CSSF) such as high-fiber and low-saccharification (HFLS), medium-fiber and medium-saccharification (MFMS), low-fiber and high-saccharification (LFHS) CSSF on the reproductive performance of sows. Thirty-two primiparous Yorkshire sows were randomly assigned to 4 groups, 8 sows for each group. Group A was the basal diet as the control group; groups B – D were added with 6% HFLSCSSF, 6% MFMSCSSF and 6% LFHSCSSF to replace some parts of corn meal and wheat bran in the basal diet, respectively. The experimental period was from day 85 of gestation to the end of lactation (day 25 post-farrowing). The results showed that 6% LFHSCSSF addition significantly increased number of total born (alive) piglets, litter weight at birth (p < 0.05), whereas three kinds of CSSF significantly decreased backfat thickness of sows during gestation (p < 0.001), compared with the control group. Furthermore, CSSF improved the digestibility of crude protein, ether extract and fiber for sows. In addition, the levels of total cholesterol, total triglycerides, and high-density lipoprotein cholesterol in serum of sows were decreased by different kinds of CSSF. Further analysis revealed that CSSF regulated lipid metabolism through adjusting the serum metabolites such as 4-pyridoxic acid, phosphatidyl cholines and L-tyrosine. In summary, CSSF addition to the diets of sows during late gestation and lactation regulated lipid metabolism and improved reproductive performance of sows. This study provided a theoretical basis for the application of corn straw in sow diets.
A comprehensive review of the botany, phytochemistry, pharmacology, and toxicology of Murrayae Folium et Cacumen
Authors: Qi Yue , Wang Lin , Wang Na , Wang Siyi , Zhu Xu , Zhao Tie , Jiang Qinghua
Published: March 2024

Ethnopharmacological relevance: Murrayae Folium et Cacumen (MFC) is a plant considered to be a traditional Chinese medicine with culinary value as well. The dry leaves and twigs of Murraya paniculata and M. exotica are used to treat stomach aches, rheumatism, toothaches, swelling, and insect and snake bites. They are also used to prepare spicy chicken dishes.

Aim of the review: This review comprehensively summarizes the available information on the botanical characterization, phytochemistry, pharmacological activities, pharmacodynamics, pharmacokinetics, and toxicity of MFC.

Methods: Relevant scientific literature up to August 2023 was included in the study. Chinese and English studies on MFC were collected from databases, including PubMed, Elsevier, Web of Science, Springer, Science Direct, Wiley, ACS, and CNKI (Chinese). Doctoral and Master’s dissertations were also included.

Results: In total, 720 compounds have been identified and reported in the literature, including flavonoids, coumarins, alkaloids, sterols, phenylpropenols, organic acids, spirocyclopentenones, and volatile oils. Flavonoids and coumarins are the two most important bioactive compounds responsible for these pharmacological activities. MFC has anti-inflammatory, anti-bacterial, anti-microbial, anti-diabetic, anti-tumor, anti-oxidant, anti-depressant, potential anti-Alzheimer’s disease, chondroprotective, and analgesic properties. The pharmacological effects include interrupting the STAT3/NF-κB/COX-2 and EGFR signaling pathways, downregulating EpCAM expression, inhibiting NF-κB and ERK signals, inhibiting the EP/cAMP/PKA signaling pathway and miR-29a/Wnt/β-catenin signaling activity, and upregulating Foxo3a expression.

Conclusion: This review demonstrates that the chemical constituents, pharmacological activities, pharmacodynamics, pharmacokinetics, and toxicity of MFC support its use in traditional Chinese botanical medicines. MFC contains a wide range of chemical compounds. Flavonoids and coumarins promote strong pharmacological activity and, are low-toxicity natural phytomedicines that are widely used in medicine, food, ornamentation, and cosmetics, making MFC a promising compound for development and use in the treatment of several medical conditions.
Phytochemical analysis and anticancer effect of Camellia oleifera bud ethanol extract in non-small cell lung cancer A549 cells
Authors: Niu Jingming , Jia Xiaoyan , Yang Nian , Ran Yuanquan , Wu Xia , Ding Furong , Tang Dongxin , Tian Minyi
Published: March 2024

Camellia oleifera is a medicine food homology plant widely cultivated in the Yangtze River Basin and southern China due to its camellia oil. Camellia oleifera bud and fruit exist simultaneously, and its bud is largely discarded as waste. However, C. oleifera bud has been used in traditional Chinese medicine to treat a variety of ailments. Thus, the purpose of this study was to identify the chemical components of C. oleifera bud ethanol extract (EE) and first evaluate its anticancer effects in non-small cell lung cancer A549 cells. Based on UHPLC-Q-Orbitrap-MS analysis, seventy components were identified. For anticancer activity, C. oleifera bud EE had remarkable cytotoxic effect on non-small cell lung cancer A549 (IC50: 57.53 ± 1.54 μg/mL) and NCI-H1299 (IC50: 131.67 ± 4.32 μg/mL) cells, while showed lower cytotoxicity on non-cancerous MRC-5 (IC50 > 320 μg/mL) and L929 (IC50: 179.84 ± 1.08 μg/mL) cells. It dramatically inhibited the proliferation of A549 cells by inducing cell cycle arrest at the G1 phase. Additionally, it induced apoptosis in A549 cells through a mitochondria-mediated pathway, which decreased mitochondrial membrane potential, upregulated Bax, activated caspase 9 and caspase 3, and resulted in PARP cleavage. Wound healing and transwell invasion assays demonstrated that C. oleifera bud EE inhibited the migration and invasion of A549 cells in a dose-dependent manner. The above findings indicated that C. oleifera bud EE revealed notable anticancer effects by inhibiting proliferation, inducing apoptosis, and suppressing migration and invasion of A549 cells. Hence, C. oleifera bud ethanol extract could serve as a new source of natural anticancer drugs.
Dose reduction of biologics in patients with plaque psoriasis: a review
Authors: van Riel C. A. M. , Michielsens C. A. J. , van Muijen M. E. , van der Schoot L. S. , van den Reek J. M. P. A. , de Jong E. M. G. J.
Published: March 2024

Dose reduction (DR) of first-generation biologics for plaque psoriasis (TNF-alpha inhibitors (i) and interleukin (IL)-12/23i) has been described in a previous scoping review. The literature on the DR of the newest generation of biologics (IL-17/23i) was scarce. The current review provides a literature update on the previous scoping review on the DR of all biologics, including the newest generation, with a focus on the uptake and implementation of DR in practice. The current literature search on DR revealed 14 new articles in addition to those in the previous review. Four of the newly found articles tested DR strategies, mostly focusing on first-generation biologics; only guselkumab (IL-23i) was included in one study. The other 10 studies showed data on regaining response after failure of DR, safety, cost-effectiveness, and uptake and implementation, as well as information about IL-17/23i. The eligibility criteria to start DR included both absolute and relative Psoriasis Area and Severity Index (PASI) scores (PASI ≤3/≤5/PASI 75–100) and/or Dermatology Life Quality Index (DLQI) ≤3/≤5, or BSA ≤1/≤2, or Physician Global Assessment (PGA) ≤1/0–2 during a period ranging from 12 weeks to ≥1 year. Most studies used PASI ≤5 and/or DLQI ≤5 or PGA ≤1 for ≥6 months. DR strategies were mostly performed by stepwise interval prolongation in two steps (to 67% of the standard dose, followed by 50%). Some studies of IL-17/23i reduced the dose to ±25%. The tested DR strategies on stepwise or fixed DR on TNF-αi and IL-12/23i (three studies), as well as one “on-demand” dosing study on IL-23i guselkumab, were successful. In the case of relapse of DR on TNF-αi and IL-12/23i, clinical effectiveness was regained by retreatment with the standard dose. All studies showed substantial cost savings with the biologic DR of TNF-αi and IL-12/23i. The identified barriers against the implementation of DR were mainly a lack of guidelines and scientific evidence on effectiveness and safety, and a lack of time and (technical) support. The identified facilitators were mainly clear guidelines, feasible protocols, adequate education of patients and physicians, and cost reduction. In conclusion, DR seems promising, but a research gap still exists in randomized, prospective studies testing DR strategies, especially of IL-17/23i, hampering the completion of guidelines on DR. Taking into account the identified barriers and facilitators most likely results in a more successful implementation of biologic DR in practice.
Improving the maize crop row navigation line recognition method of YOLOX
Authors: Gong Hailiang , Zhuang Weidong , Wang Xi
Published: March 2024

The accurate identification of maize crop row navigation lines is crucial for the navigation of intelligent weeding machinery, yet it faces significant challenges due to lighting variations and complex environments. This study proposes an optimized version of the YOLOX-Tiny single-stage detection network model for accurately identifying maize crop row navigation lines. It incorporates adaptive illumination adjustment and multi-scale prediction to enhance dense target detection. Visual attention mechanisms, including Efficient Channel Attention and Cooperative Attention modules, are introduced to better extract maize features. A Fast Spatial Pyramid Pooling module is incorporated to improve target localization accuracy. The Coordinate Intersection over Union loss function is used to further enhance detection accuracy. Experimental results demonstrate that the improved YOLOX-Tiny model achieves an average precision of 92.2 %, with a detection time of 15.6 milliseconds. This represents a 16.4 % improvement over the original model while maintaining high accuracy. The proposed model has a reduced size of 18.6 MB, representing a 7.1 % reduction. It also incorporates the least squares method for accurately fitting crop rows. The model showcases efficiency in processing large amounts of data, achieving a comprehensive fitting time of 42 milliseconds and an average angular error of 0.59°. The improved YOLOX-Tiny model offers substantial support for the navigation of intelligent weeding machinery in practical applications, contributing to increased agricultural productivity and reduced usage of chemical herbicides.
Integrative transcript to proteome analysis of barley during Ramularia collo-cygni leaf spot development identified several proteins that are related to fungal recognition and infection responses
Authors: Lemcke René , Kamble Manoj , Schneider Sebastian , Lyngkjær Michael F. , Radutoiu Simona , Wienkoop Stefanie
Published: March 2024

Introduction

Ramularia leaf spot (RLS) disease is a growing threat to barley cultivation, but with no substantial resistance identified to date. Similarly, the understanding of the lifestyle of Ramularia collo-cygni (Rcc) and the prediction of RLS outbreak severity remain challenging, with Rcc displaying a rather untypical long endophytic phase and a sudden change to a necrotrophic lifestyle. The aim of this study was to provide further insights into the defense dynamics during the different stages of colonization and infection in barley in order to identify potential targets for resistance breeding.

Methods

Utilizing the strength of proteomics in understanding plant–pathogen interactions, we performed an integrative analysis of a published transcriptome dataset with a parallel generated proteome dataset. Therefore, we included two spring barley cultivars with contrasting susceptibilities to Rcc and two fungal isolates causing different levels of RLS symptoms.

Results

Interestingly, early responses in the pathogen recognition phase of the host were driven by strong responses differing between isolates. An important enzyme in this process is a xylanase inhibitor, which protected the plant from cell wall degradation by the fungal xylanase. At later time points, the differences were driven by cultivar-specific responses, affecting mostly features contributing to the pathogenesis- and senescence-related pathways or photosynthesis.

Discussion

This supports the hypothesis of a hemibiotrophic lifestyle of Rcc, with slight differences in trophism of the two analyzed isolates. The integration of these data modalities highlights a strength of protein-level analysis in understanding plant–pathogen interactions and reveals new features involved in fungal recognition and susceptibility in barley cultivars.
Multi-omics analysis provides new insights into the changes of important nutrients and fructose metabolism in loquat bud sport mutant
Authors: Song Hai-yan , Zhao Ke , Pei Yan-Gang , Chen Hong-xu , Wang Xiao-an , Jiang Guo-Liang , Xie Hong-Jiang , Chen Dong , Gong Rong-gao
Published: March 2024

Bud sport is a common and stable somatic variation in perennial fruit trees, and often leads to significant modification of fruit traits and affects the breeding value. To investigate the impact of bud sport on the main metabolites in the fruit of white-fleshed loquat, we conducted a multi-omics analysis of loquat fruits at different developmental stages of a white-fleshed bud sport mutant of Dongting loquat (TBW) and its wild type (TBY). The findings from the detection of main fruit quality indices and metabolites suggested that bud sport resulted in a reduction in the accumulation of carotenoids, fructose, titratable acid and terpenoids at the mature stage of TBW, while leading to the accumulation of flavonoids, phenolic acids, amino acids and lipids. The comparably low content of titratable acid further enhances the balanced and pleasent taste profile of TBW. Expression patterns of differentially expressed genes involved in fructose metabolism exhibited a significant increase in the expression level of S6PDH (EVM0006243, EVM0044405) prior to fruit maturation. The comparison of protein sequences and promoter region of S6PDH between TBY and TBW revealed no structural variations that would impact gene function or expression, indicating that transcription factors may be responsible for the rapid up-regulation of S6PDH before maturation. Furthermore, correlation analysis helped to construct a comprehensive regulatory network of fructose metabolism in loquat, including 23 transcription factors, six structural genes, and nine saccharides. Based on the regulatory network and existing studies, it could be inferred that transcription factors such as ERF, NAC, MYB, GRAS, and bZIP may promote fructose accumulation in loquat flesh by positively regulating S6PDH. These findings improve our understanding of the nutritional value and breeding potential of white-fleshed loquat bud sport mutant, as well as serve as a foundation for exploring the genes and transcription factors that regulate fructose metabolism in loquat.
A comparative study on fundamental movement skills among children with autism spectrum disorder and typically developing children aged 7–10
Authors: Dong Liangshan , Fan Rong , Shen Bo , Bo Jin , Pang Yanli , Song Yu
Published: March 2024

Background

Autism Spectrum Disorder (ASD) is a neurodevelopmental condition with unique differences in social interaction, communication, and a spectrum of behavioral characteristics. In the past, motor disturbance in individuals with ASD has not been considered a significant core deficit due to the predominant focus on sociability and communication issues. However, recent studies indicate that motor deficits are indeed associated with the fundamental symptoms of ASD. As there is limited research on the motor behavior of children with ASD, particularly in China, the objective of this study is to investigate the development of fundamental movement skills (FMS) in children with ASD and compare them to typically developing children.

Method

The study recruited 108 children with ASD (87 boys, 21 girls) aged 7–10 years from two special education rehabilitation centers in Wuhan, China. For comparison, a control group of 108 typically developing children, matched by age and gender, was randomly selected from three local primary schools. FMS were assessed using the Movement Assessment Battery for Children - Second Edition (MABC-2), which evaluates manual dexterity, aiming and catching, as well as static and dynamic balance. Group differences on MABC-2 percentile scores were analyzed using descriptive statistics and Mann–Whitney U test. Effect sizes were also calculated for practical significance.

Results

Findings from the study showed that a significant majority, around 80%, of children with ASD either displayed motor challenges or were at risk of developing such delays. When comparing to their typically developing peers, children with ASD scored notably lower in areas of manual dexterity, ball skills, and both static and dynamic balance (with all these findings being statistically significant at p < 0.001). Interestingly, gender did not show a significant influence on these results (p > 0.05).

Conclusion

In addition to addressing the other skill development areas outlined in the diagnostic manual for ASD, clinicians diagnosing and treating children with ASD should also assess the presence of motor skill development. For individuals with ASD who have co-existing motor difficulties, it is essential to offer evidence-based interventions tailored to their specific needs.
Interventions addressing the impostor phenomenon: a scoping review
Authors: Para Emma , Dubreuil Philippe , Miquelon Paule , Martin-Krumm Charles
Published: March 2024

Introduction

The Impostor Phenomenon (IP) refers to a psychological experience characterized by unjustified feelings of intellectual and professional fraud, accompanied by the fear of not maintaining performance and of being exposed. IP is receiving increasing attention in the fields of psychological health at work and occupational psychology as well as among the general public, since it affects the functioning of both individuals and organizations. The aim of this scoping review is to map the range of interventions that have been conducted to address IP among individuals experiencing it in a professional context.

Methods

The search and selection process to identify relevant reports was conducted using the PRISMA-ScR methodology and JBI recommendations and resulted in the selection of 31 studies.

Results

The results reported concerning the characteristics of the studies, the interventions described, and the effects identified are heterogeneous. More than half of the studies used research designs (experimental, pre-experimental, exploratory, etc.). Two major types of intervention emerge: training and counseling. The effectiveness of the interventions varies according to the evaluation methodology that was used, although most authors conclude that the proposed intervention is relevant.

Discussion

In light of these results, recognizing and educating individuals regarding the various manifestations of IP, as well as offering support in a group context, appear to be primary intervention levers. Future intervention proposals should explore psychosocial and educational influences as well as the impact of the immediate environment on IP-related beliefs.
Suggestive answers strategy in human-chatbot interaction: a route to engaged critical decision making
Authors: Yamamoto Yusuke
Published: March 2024

In this study, we proposed a novel chatbot interaction strategy based on the suggestive ending of answers. This strategy is inspired by the cliffhanger ending narrative technique, which ends a story without specifying conclusions to spark readers' curiosity as to what will happen next and is often used in television series. Common chatbots provide relevant and comprehensive answers to users' questions. In contrast, chatbots with our proposed strategy end their answers with hints potentially interest-triggering users. The suggestive ending strategy aims to stimulate users' inquisition for critical decision-making, relating to a psychological phenomenon where humans are often urged to finish the uncompleted tasks they have initiated. We demonstrated the implication of our strategy by conducting an online user study involving 300 participants, where they used chatbots to perform three decision-making tasks. We adopted a between-subjects factorial experimental design and compared between the following UIs: (1) plain chatbot—it provides a generated answer when participants issue a question; (2) expositive chatbot—it provides a generated answer for a question, adding short summaries of a positive and negative person's opinion for the answer; (3) suggestive chatbot—it provides a generated answer for a question, which ends with a suggestion of a positive and negative person for the answer. We found that users of the suggestive chatbot were inclined to ask more questions to the bot, engage in prolonged decision-making and information-seeking actions, and formulate their opinions from various perspectives. These findings vary with the users' experience with plain and expositive chatbots.
Decoding the secrets of longevity: unraveling nutraceutical and miRNA-Mediated aging pathways and therapeutic strategies
Authors: Salama Rania M. , Eissa Nermin , Doghish Ahmed S. , Abulsoud Ahmed I. , Abdelmaksoud Nourhan M. , Mohammed Osama A. , Abdel Mageed Sherif S. , Darwish Samar F.
Published: March 2024

MicroRNAs (miRNAs) are short RNA molecules that are not involved in coding for proteins. They have a significant function in regulating gene expression after the process of transcription. Their participation in several biological processes has rendered them appealing subjects for investigating age-related disorders. Increasing data indicates that miRNAs can be influenced by dietary variables, such as macronutrients, micronutrients, trace minerals, and nutraceuticals. This review examines the influence of dietary factors and nutraceuticals on the regulation of miRNA in relation to the process of aging. We examine the present comprehension of miRNA disruption in age-related illnesses and emphasize the possibility of dietary manipulation as a means of prevention or treatment. Consolidating animal and human research is essential to validate the significance of dietary miRNA control in living organisms, despite the abundance of information already provided by several studies. This review elucidates the complex interaction among miRNAs, nutrition, and aging, offering valuable insights into promising areas for further research and potential therapies for age-related disorders.
Designing payload and spaceflight operations for plants from extreme terrestrial environments
Authors: Zupanska Agata K. , Lockwood Emily , Zhang Ye , Haveman Natasha J. , Carver John A. , Spern Charles W. , Senyk Emily , Richards Jeffrey T. , Koss Lawrence L. , Dimapilis Dinah I. , McDaniel Stuart F.
Published: March 2024

Terrestrial plants from the very limits of life are likely to harbor genes that confer an advantage in human space exploration. These plants are seemingly capable of performing mission critical functions in spaceflight and on extraterrestrial farms while informing directed gene manipulation in target plant species. However, their adaptations to physiologically extreme habitats may hinder the efficacy of routine laboratory techniques for model plants. We here present the development of Antarctic moss Ceratodon purpureus payload and flight operations for the ANT1 Radiation Tolerance Experiment with Moss in Orbit on the Space Station (ARTEMOSS) experiment to the International Space Station (ISS) given limited physical space and crew time. We demonstrate that the hydrophobic surface of Antarctic moss impedes chemical tissue fixation and precludes the use of RNAlater coupled with payload hardware deployed in standard plant spaceflight experiments. We show that deep-freezing the moss tissue on Petri plates provides adequate tissue fixation and allows the extraction of high-quality RNA suitable for gene expression profiling. We replaced hardware with stacks of Petri plates housing Antarctic moss and chemical fixation with deep-freezing in a cryogenic GLACIER freezer. Our design can be translated to other plant species to expand current experimentation techniques with plants from extreme terrestrial environments in order to advance human space exploration.
How Do Lumpfish Protect Themselves Against Viruses?
Authors: Rao Shreesha S. , Haugland Gyri T.
Published: March 2024

For many people, a delicious salmon dish satisfies their taste buds. Salmon farming is a big industry, providing food for millions of people every day. However, the journey of this delicious meal from the ocean to your plate depends on lumpfish, a cool-looking fish that protects farmed salmon by eating sea lice. Sea lice are small parasites known to attack salmon and can cause disease if not removed. Since lumpfish are vulnerable to diseases, it is crucial to understand more about this organism’s complex immune system, as this will help keep them healthy so they can then do their important “job” of eating sea lice. In this article, we will explain how we study the way the fascinating lumpfish defends itself against diseases.
Cecropin AD reduces viral load and inflammatory response against H9N2 avian influenza virus in chickens
Authors: Zhang Taiming , Liu Zhenyi , Zhi Yan , Zhao Xinping , Du Mengze , Zhang Qian , Zhang Tao , Hu Ge
Published: March 2024

Introduction

This study focuses on evaluating the therapeutic efficacy of cecropin AD, an antimicrobial peptide, against H9N2 avian influenza virus (AIV) in chickens. Given the global impact of H9N2 AIV on poultry health, identifying effective treatments is crucial.

Methods

To assess the impact of cecropin AD, we conducted in vivo experiments involving 108 5-week-old chickens divided into control, infected, and various treatment groups based on cecropin AD dosage levels (high, medium, and low). The methodologies included hemagglutination (HA) tests for viral titers, histopathological examination and toluidine blue (TB) staining for lung pathology, real-time PCR for viral detection, and enzyme-linked immunosorbent assays for measuring serum levels of inflammatory markers.

Results

The findings revealed that cecropin AD substantially reduced lung pathology and viral load, especially at higher dosages, comparing favorably with the effects seen from conventional treatments. Moreover, cecropin AD effectively modulated mast cell activity and the levels of inflammatory markers such as IL-6, TNF-α, IFN-γ, and 5-HT, indicating its potential to diminish inflammation and viral spread.

Discussion

Cecropin AD presents a significant potential as an alternative treatment for H9N2 AIV in chickens, as evidenced by its ability to lessen lung damage, decrease viral presence, and adjust immune responses. This positions cecropin AD as a promising candidate for further exploration in the management of H9N2 AIV infections in poultry.
Effect of a microencapsulated phyto/phycogenic blend supplementation on growth performance, processing parameters, meat quality, and sensory profile in male broilers
Authors: Mullenix Garrett J. , Greene Elizabeth S. , Ramser Alison , Maynard Clay , Dridi Sami
Published: March 2024

Powered by consumer taste, value, and preferences, natural products including phytogenics and algae are increasingly and separately used in the food systems where they have been reported to improve growth performance in poultry and livestock. The present study aimed to determine the effects of a new feed additive, microencapsulated NUQO© NEX, which contains a combination of phytogenic and phycogenic, on broiler growth performance, blood chemistry, bone health, meat quality and sensory profile. Male Cobb500 chicks (n = 1,197) were fed a 3-phase feeding intervals; 1–14d starter, 15–28d grower, and 29–40d finisher. The dietary treatments included a corn-soy basal Control (CON), basal diet supplemented with NUQO© NEX at 100 g/ton from 1 to 28d then 75 g/ton from d 28 to 40 (NEX75), and basal diet supplemented with NUQO© NEX at 100 g/ton from 1 to 40d (NEX100). The NEX100 supplemented birds had 62 g more BWG increase and 2.1-point improvement in FCR compared with CON in the finisher and overall growth phase (p < 0.05), respectively. Day 40 processing body weights and carcass weights were heavier for the NEX100 supplemented birds (p < 0.05). The incidences of muscle myopathies were also higher in NEX treatments, which could be associated with the heavier weights, but the differences were not detected to be significant. The NEX75 breast filets had more yellowness than other dietary treatments (p = 0.003) and the NEX 100 treatment reduced the levels of breast filet TBARS at 7 days-post harvest (p = 0.053). Finally, both NEX treatments reduced the incidence of severe bone (tibia and femur) lesions. In conclusion, the supplementation of the phytogenic NUQO© NEX improved finisher performance parameters, whole phase FCR, processing carcass weights, and breast filet yellowness, at varying inclusion levels.
Impact of the 2022 Energy Crisis on the Importance of Energy Performance Certificates for the Real Estate Market
Authors: Fekete Dalma Eszter , Baranyai Eszter
Published: March 2024

This study relies on a unique database covering different segments of the residential real estate market to investigate whether the value of energy performance certificates was affected by the energy crisis in 2022. Based on the relevant literature, the study uses the linear regression estimation method, in which the effect of interaction is examined using the difference-in-difference method. The study shows that energy performance certificates have a significant impact on the estimated market value of properties, both by individual category and in groups. While in 2022 Q2, before the residential energy price increase, an energy-inefficient property cost 12 per cent less on average compared to a property with an average energy rating, controlling for other factors, that difference increased to 20 per cent after the outbreak of the energy crisis. It may therefore be worthwhile for Hungary to consider taking measures to improve the energy efficiency of buildings as soon as possible.
Complementing Money Functions: Central Bank Digital Currencies and Currency Competition
Authors: Dötsch Jörg J. , Ginter Tamás
Published: March 2024

Central bank digital currencies (CBDCs) are currently one of the most vital issues in monetary policy worldwide. While several aspects of the emergence of digital currencies have been addressed in the respective literature, little focus has been directed to one important underlying parameter: the new role of technology in the digital age, which complements the traditional money functions and hence may exert an effect on currency competition. In this paper, we first provide an overview of money functions, currency competition and digital money. We then state that with the emergence of CBDCs, technology has become a new and important functional parameter born by the CBDC itself. We conclude that while technological competition has arisen as a factor of currency competition, stability remains its most decisive factor.
Minimizing Voltage Ripple of a DC Microgrid via a Particle-Swarm-Optimization-Based Fuzzy Controller
Authors: Zolfaghari Hussein , Karimi Hossein , Ramezani Amin , Davoodi Mohammadreza
Published: March 2024

DC microgrids play a crucial role in both industrial and residential applications. This study focuses on minimizing output voltage ripple in a DC microgrid, including power supply resources, a stochastic load, a ballast load, and a stabilizer. The solar cell serves as the power supply, and the stochastic load represents customer demand, whereas the ballast load includes a load to safeguard the boost circuits against the overvoltage in no-load periods. The stabilizer integrates components such as electrical vehicle batteries for energy storage and controlling long-time ripples, supercapacitors for controlling transient ripples, and an over-voltage discharge mechanism to prevent overcharging in the storage. To optimize the charging and discharging for batteries and supercapacitors, a multi-objective cost function is defined, consisting of two parts—one for ripple minimization and the other for reducing battery usage. The battery charge and discharge are considered in the objective function to limit its usage during transient periods, providing a mechanism to rely on the supercapacitor and protect the battery. Particle swarm optimization is employed to fine-tune the fuzzy membership function. Various operational scenarios are designed to showcase the DC microgrid’s functionality under different conditions, including scenarios where production exceeds and falls below consumption. The study demonstrates the improved performance and efficiency achieved by integrating a PSO-based fuzzy controller to minimize voltage ripple in a DC microgrid and reduce battery wear. Results indicate a 42% enhancement in the integral of absolute error of battery current with our proposed PSO-based fuzzy controller compared to a conventional fuzzy controller and a 78% improvement compared to a PI controller. This translates to a respective reduction in battery activity by 42% and 78%.
Exploring the Importance of Environmental Complexity for Newly Hatched Zebrafish
Authors: Santacà Maria , Gatto Elia , Dadda Marco , Bruzzone Matteo , Dal Maschio Marco , Bisazza Angelo
Published: March 2024

The effects of an early impoverished social or physical environment on vertebrate neural development and cognition has been known for decades. While existing studies have focused on the long-term effects, measuring adult cognitive phenotypes, studies on the effects of environmental complexity on the early stages of development are lacking. Zebrafish (Danio rerio) hatchlings are assumed to have minimal interaction with their environment and are routinely reared in small, bare containers. To investigate the effects of being raised under such conditions on development of behaviour and cognition, hatchlings housed for 10 days in either an enriched or a standard environment underwent two cognitive tasks. The results were mixed. Subjects of the two treatments did not differ in performance when required to discriminate two areas. Conversely, we found a significant effect in a number discrimination task, with subjects from impoverished condition performing significantly worse. In both experiments, larvae reared in impoverished environment showed a reduced locomotor activity. Given the effects that enrichment appears to exert on larvae, a third experiment explored whether hatchlings exhibit a spontaneous preference for more complex environments. When offered a choice between a bare setting and one with objects of different shapes and colors, larvae spent over 70% of time in the enriched sector. Deepening these effects of an early impoverished environment on cognitive development is crucial for the welfare of captive zebrafish populations and for enhancing the quality and reliability of studies on larval zebrafish.
Salmonella Control in Swine: A Thoughtful Discussion of the Pre- and Post-Harvest Control Approaches in Industrialized Countries
Authors: Carvajal Ana , Kramer Melvin , Argüello Héctor
Published: March 2024

Pork is among the major sources of human salmonellosis in developed countries. Since the 1990s, different surveys and cross-sectional studies, both national and international (i.e., the baseline studies performed in the European Union), have revealed and confirmed the widespread non-typhoidal Salmonella serotypes in pigs. A number of countries have implemented control programs with different approaches and degrees of success. The efforts could be implemented either at farms, in post-harvest stages, or both. The current review revises the current state of the art in Salmonella in swine, the control programs ongoing or conducted in the past, and their strengths and failures, with particular attention to the weight of pre- and post-harvest control and the implications that both have for the success of interventions or mitigation after outbreaks. This review provides a novel perspective on Salmonella control in swine, a matter that still includes uncertainties and room for improvement as a question of public health and One Health.
Characterization of Typical Behaviors of Mares in the Opening Phase of Parturition—Influence of Parity and Dystocia
Authors: Lindinger Hannah , Wehrend Axel
Published: March 2024

The identification of typical behaviors in stage I of parturition, the opening phase, can be used to improve birth monitoring in the mare. Therefore, this study aimed to comprehensively analyze mare behavior during the opening phase. Real-time recordings of 66 births involving 56 warmblood mares were analyzed using camera footage. Behaviors such as increased locomotor activity, pawing with front hooves, lifting the tail, rolling, kicking with the hind legs toward the abdomen, and looking at the abdomen increased significantly (p < 0.001) in the four hours preceding parturition. Within the last hour of the observation period, a statistically significant change was observed for the duration of lying in the sternal and lateral position (p < 0.001). Significant correlations were observed between parity and the total number of repetitions of lying in the sternal position (p < 0.05). Furthermore, the birth process influenced the repetitions of lying in the lateral position (p < 0.05). These findings indicate distinct behavioral patterns during the opening phase of parturition, which were evident across the observed mares. Nonetheless, notable individual differences were also identified among the mares.
From Polymeric Nanoformulations to Polyphenols—Strategies for Enhancing the Efficacy and Drug Delivery of Gentamicin
Authors: Bārzdiņa Ance , Plotniece Aiva , Sobolev Arkadij , Pajuste Karlis , Bandere Dace , Brangule Agnese
Published: March 2024

Gentamicin is an essential broad-spectrum aminoglycoside antibiotic that is used in over 40 clinical conditions and has shown activity against a wide range of nosocomial, biofilm-forming, multi-drug resistant bacteria. Nevertheless, the low cellular penetration and serious side effects of gentamicin, as well as the fear of the development of antibacterial resistance, has led to a search for ways to circumvent these obstacles. This review provides an overview of the chemical and pharmacological properties of gentamicin and offers six different strategies (the isolation of specific types of gentamicin, encapsulation in polymeric nanoparticles, hydrophobization of the gentamicin molecule, and combinations of gentamicin with other antibiotics, polyphenols, and natural products) that aim to enhance the drug delivery and antibacterial activity of gentamicin. In addition, factors influencing the synthesis of gentamicin-loaded polymeric (poly (lactic-co-glycolic acid) (PLGA) and chitosan) nanoparticles and the methods used in drug release studies are discussed. Potential research directions and future perspectives for gentamicin-loaded drug delivery systems are given.
Phylogeny of Transferable Oxazolidinone Resistance Genes and Homologs
Authors: Kardos Gábor , Laczkó Levente , Kaszab Eszter , Timmer Bálint , Szarka Krisztina , Prépost Eszter , Bányai Krisztián
Published: March 2024

Oxazolidinone resistance, especially transmissible resistance, is a major public health concern, and the origin of this resistance mechanism is not yet resolved. This study aims to delve into the phylogenetic origin of the transmissible oxazolidinone resistance mechanisms conferring cross-resistance to other drugs of human and veterinary importance. The amino acid sequences of the five cfr ribosomal methylases and optrA and poxtA were used as queries in searches against 219,549 bacterial proteomes in the NCBI RefSeq database. Hits with >40% amino acid identity and >80% query coverage were aligned, and phylogenetic trees were reconstructed. All five cfr genes yielded highly similar trees, with rlmN housekeeping ribosomal methylases located basal to the sister groups of S-adenosyl-methionine-dependent methyltransferases from various Deltaproteobacteria and Actinomycetia, including antibiotic-producing Streptomyces species, and the monophyletic group of cfr genes. The basal branches of the latter contained paenibacilli and other soil bacteria; they then could be split into the clades [cfr(C):cfr(E)] and [[cfr:cfr(B)]:cfr(D)], always with different Bacillaceae in their stems. Lachnospiraceae were encountered in the basal branches of both optrA and poxtA trees. The ultimate origin of the cfr genes is the rlmN housekeeping ribosomal methylases, which evolved into a suicide-avoiding methylase in antibiotic producers; a soil organism (Lachnospiraceae, Paenibacilli) probably acted as a transfer organism into pathogenic bacteria. In the case of optrA, the porcine pathogenic Streptococcus suis was present in all branches, while the proteins closest to poxtA originated from Clostridia.
Fucoidan Supplementation Improves Antioxidant Capacity via Regulating the Keap1/Nrf2 Signaling Pathway and Mitochondrial Function in Low-Weaning Weight Piglets
Authors: Yin Chenggang , Bi Qingyue , Chen Wenning , Wang Chengwei , Castiglioni Bianca , Li Yanpin , Sun Wenjuan , Pi Yu , Bontempo Valentino , Li Xilong , Jiang Xianren
Published: March 2024

Fucoidan (FC) is known for its antioxidant properties, but it has unclear effects and mechanisms on weaned piglets. Two experiments were conducted to determine the optimal FC dosage in piglet diets and its protective effect against lipopolysaccharide (LPS)-induced oxidative stress. In experiment one, 24 low weight weaned piglets were randomly assigned to four dietary treatments: a basal diet (FC 0), or a diet supplemented with 150 (FC 150), 300 (FC 300), or 600 mg/kg FC (FC 600). In experiment two, 72 low-weaning weight piglets were randomly allocated into four treatments: a basal diet (CON), or 300 mg/kg of fucoidan added to a basal diet challenged with LPS (100 µg LPS/kg body weight) or not. The results showed that FC treatments increased the G:F ratio, and dietary FC 300 reduced the diarrhea incidence and increased the plasma IGF-1 concentrations. In addition, FC 300 and FC 600 supplementation increased the plasma SOD activity and reduced the plasma MDA concentration. LPS challenge triggered a strong systemic redox imbalance and mitochondrial dysfunction. However, dietary FC (300 mg/kg) supplementation increased the activity of antioxidant enzymes, including SOD, decreased the MDA concentration in the plasma and liver, down-regulated Keap1 gene expression, and up-regulated Nrf2, CAT, MFN2, SDHA, and UQCRB gene expression in the liver. These results indicated that dietary fucoidan (300 mg/kg) supplementation improved the growth performance and antioxidant capacity of low-weaning weight piglets, which might be attributed to the modulation of the Keap1/Nrf2 signaling pathway and the mitochondrial function in the liver.