bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Gypsum and Vermicompost on Aggregate Stability of Bauxite against Liquefaction
Authors: Zaini Muhammad Syamsul Imran , Hasan Muzamir , Pahrol Muhammad Fat-Hi Al Juwaini
Published: November 2022

Improving stability and strengthening aggregate formation of bauxite in bulk cargoes to reduce the danger of liquefaction remains a challenge. The influence of gypsum and vermicompost on the alkali-aggregate reaction of bauxite against liquefaction in solid bulk cargoes are investigated using wet sieving, the modified Le Bissonnais' (LB), flow table, particle size distribution, specific gravity, scanning emission microscopy, and energy dispersive x-ray. Its effect on the aggregate stability of bauxite was investigated by including 2% and 4% gypsum and 4% and 10% vermicompost by weight of dry bauxite, respectively. The study of the soil samples suggested that the ideal condition for boosting aggregate stability and lowering the danger of bauxite liquefaction is at a level of 6% gypsum and 4% vermicompost, respectively. The addition of gypsum and vermicompost improves PSD at 2.5mm (20.38% increment), specific gravity (20.67% reduction), erosion resistance (40.00% increment), Mean Weight Diameter (75.51% increment), Transportable Moisture Limit (26.97% increment), and changes the microstructure of the bauxite from a sheet like structure to granular macro aggregated structure. The results shown that the addition of gypsum and vermicompost to bauxite had a direct effect on micromorphology and aggregate size distribution, resulting in an increase in aggregate stability and structure and a reduction in the risk of liquefaction.
Single-loop and "Y"-shaped Double-loop Tightrope fixation for fresh Rockwood III-V acromioclavicular joint dislocation: an observational study lasting at least 3 years (3-6 years)
Authors: shen zheyuan , zhang zhanfeng , sun jianwei , deng minjun
Published: November 2022

Backgrounds

To compare the reduction effect of single loop and double loop in the treatment of acute acromioclavicular dislocation, postoperative complications and the effect of shoulder function recovery, so as to determine the best fixation method for acromioclavicular.

Methods

Patients with acute acromioclavicular dislocation treated with TightRope fixation in our hospital from January 2014 to May 2019 were reviewed. According to the different fixation methods, it can be divided into single loop group and double loop group. The difference and difference ratio of coracoclavicular spacing between the affected side and the healthy side at the 1st day, 3rd week, 6th week, 3rd month, 6th month, 1st year, 2nd year and the last follow-up were compared between the two groups, as well as the postoperative visual analog scale pain score (VAS), American Shoulder and Elbow Surgeon score(ASES) and constant-Murley score.

Results

On the 1st postoperative day, there was no significant difference in the reduction effect between the single loop group and the double loop group (P = 0.90,P = 0.93). 3 weeks after surgery, although the overall difference and difference rate of CCD between the two groups were different (P = 0.00,P = 0.00), there was no significant difference between type ⅲ and type ⅳ in terms of injury type (Type ⅲ P = 0.13,P = 0.16; Type ⅳ p = 0.22, p = 0.20;). Since 6 weeks after surgery, there were significant statistical differences in CCD difference rate between single loop group and double loop group.

Conclusions

Both single loop and double loop Tightrope can achieve satisfactory results in the treatment of fresh Rockwood ⅲ - ⅴ acromioclavicular dislocation. The single-loop group has the risk of postoperative reduction and loss.
Enhanced Postoperative Analgesic Effect of Hydromorphone Hydrochloride and Decreased Incidence of Pruritus via Continuous Low-dose Pumping of Nalmefene: A Randomized Controlled Study
Authors: Wang Ye , Zhao Lin , Wu Meng , An Qi , Guo Qianqian , Fan Chunling , Guo Zhenggang
Published: November 2022

Background: Hydromorphone hydrochloride has a satisfactory effect on postoperative analgesia for patients with colorectal cancer, yet accompanied by a relatively incidence of adverse events. Low-dose naloxone combined with opioids for Patient Controlled Analgesia (PCA) can reduce the incidence of drug-related adverse events. Meanwhile, Nalmefene is a more selective opioid receptor antagonist than naloxone. This study hypothesized that low-dose nalmefene combined with hydromorphone hydrochloride for PCA could enhance the analgesic effect of the drug and reduce the incidence of adverse events.
Methods The present research was designed as a randomized, double-blind, controlled study. Ninety-nine patients undergoing selective radical resection of colorectal cancer were enrolled and randomly divided into three groups. The postoperative analgesia plan of the three groups was Hydromorphone hydrochloride (0.15mg/kg) added to 100 ml of normal saline, among which 2μg/kg of Nalmefene was added to group N1, 0.5μg/kg Nalmefene to group N2, and normal saline only in group C. The induction and maintenance plan of general anesthesia were the same in the three groups, and PCA was connected after operation. Furthermore, the following data were recorded, including the degree of pain (NRS score) at 1, 6, 12, 24 and 48h after surgery, the occurrence of nausea, vomiting and pruritus, the frequency of PCA, the time of postoperative exhaust, the time of drainage tube extubation, and the overall length of hospital stay.
Results After being approved by the Ethics Committee of our hospital, our study included ninety-three patients with written informed consent provided. Compared with group C, the NRS scores were significantly lower in group N1 at 12 and 48 hours after operation (P<0.05), but there was no significant difference in group N2. Compared with 12 hours after operation, the NRS scores of the three groups decreased at 48h after operation (P<0.05). There was no significant difference in the incidence of nausea and vomiting, but a difference was observed in the incidence of pruritus among three groups(P<0.05).
ConclusionNalmefene at the dosage of 2μg/kg enhances the postoperative analgesic effect of hydromorphone hydrochloride and reduces the occurrence of postoperative pruritus.
Trial Registration: The trial was registered on the Chinese Clinical Trial Registry (Registration number: ChiCTR2000033520, date: 03/06/2020).
Impact of financial support on treatment outcomes of multidrug-resistant tuberculosis: a population-based, retrospective cohort study in China
Authors: Chen Yong , Zhang Yi , Wu Zheyuan , Xu Biao , Chen Jing , Sha Wei , Liu Xiaoxia , Shen Xin
Published: November 2022

Background To date, the treatment success rate of Multidrug-resistant tuberculosis (MDR-TB) is low. The prolonged treatment course and expensive second-line anti-tuberculosis drugs (SLDs) for treating MDR-TB could lead to heavy financial expenditure. This economic barrier might result in poor adherence, lost to follow-up and treatment failure. Therefore, provision of financial support might improve treatment outcomes of MDR-TB. This study investigated the association between a subsidy policy and treatment outcomes of MDR-TB patients. Methods In this population-based, retrospective study, demographic, clinical characteristics, and the drug susceptibility profiles of all registered MDR-TB patients between 2011 and 2019 in Shanghai, China were collected. In total, 865 of 1016 patients were eligible for analysis, among which 70.6% (611/865) had treatment success. Information on the subsidies for MDR-TB treatment, which was in addition to the basic health insurance, were routinely recorded by the end of December 2021. Multivariate logistic regression adjusted for potential confounders was performed to evaluate the association between the situation of subsidies and treatment outcomes by estimating odds ratios (ORs) and 95% confidence intervals (CIs). The effect of greater/less subsidies on outcomes was further assessed using the median amount of financial support as cut-off points. Results During the study period, 74.1% (641/856) patients received subsidies for MDR-TB treatment. The median subsidies received across the treatment course was 15026 (Interquartile range, IQR, 7107–36001) Chinese Yuan (CNY), of which the reimbursement for SLDs were the major components. Those who had treatment success received significantly more subsidies (median, 17836 CNY) compared with patients with adverse outcomes (9794 CNY, P < 0.001). Benefiting from the subsidy policy was positively associated with treatment success, with an adjusted odds ratio (OR) equal to 2.95 (95% confidence interval [CI], 2.03–4.28). Among 641 patients who received subsidies, the OR comparing those with greater and less reimbursement was 1.74 (95% CI, 1.16–2.61). Additional adjustment for baseline drug-resistance did not alter these results. Conclusions Financial support was positively associated with better treatment outcomes among MDR-TB patients. Results in this study call for further financial assistance to facilitate appropriate medical care and to improve treatment outcomes of MDR-TB.
Relevance and Experiences of HIV Testing Models towards Three 95 Targets in Tanzania
Authors: Joho Selemani , Mollel Henry A. , Kacholi Godfrey
Published: November 2022

Background
The government of Tanzania adopted the United Nation AIDS “95-95-95” strategy as a step to fast tracking HIV/AIDS free generation by 2030. The adoption was followed by expansion of HIV services to all health facility levels. The focus of the nation was to strengthen HIV testing services that is central to achieve the first 95- of UNAIDS fast-track target as adopted by Tanzania for which the second and third 95s are heavily dependent on. Achievement of first 95 was expected to drive the achievement of second 95 and third 95s.The study aimed to assess implementation of HIV testing model in increasing coverage of HIV testing at Pangani district council.
Methods
Descriptive cross-sectional study was conducted at Pangani District Council. Purposive sampling technique was used to select health care providers from health facilities providing HIV testing services. Interview and focus group discussion used to collect primary data from facility in charge, CTC in charge and staffs working in CTC unit. Data from CTC 2 data base and DHIS 2 for monitoring HIV services were used. Descriptive analysis, phenomenological analysis and ethnographic analysis were used to summarize, organize and interpret data through Atlas ti software version 7.
Results
Pangani district council tested 106,232 people between 2016 and 2020. Out of tested people, 69, 460 (65.39%) were tested at five health facility through Amref supported program and 36,772 (34.61%) tested from 17 facilities that did not get support from Amref. Among the people tested for HIV 2,843 people (2.68%) were positive where: PITC contributed 73.40%, index contributed (18.15%), PMTCT contributed (4.22%) and VCT contributed (2.78%). Training on new HIV testing strategies, motivation of staff and availability of HIV testing kits were the factors associated to achievement.
Conclusion
The council has to establish regular on job training need assessment on provision of HIT at all levels. The council should prepare plan and allocate resources to the 18 facilities that did not get support from Amref. This plan and resources will help synthesize efforts to implementation of HIV testing models. The council should find another implementation partner in HIV services to cover the facilities that do not get support from Amref. Motivation supposed to be clear in terms of process, motivational factors, time interval and criteria for being motivated
Pulmonologist performed ultrasound guided fine needle aspiration of lung lesions
Authors: Kwok Chin Tong , Yeung Yiu Cheong , Chan Yu Hong , Ho Man Ying
Published: November 2022

Background and objective:
Lung cancer is increasingly common and accurate diagnosis is important for personalized treatment. Ultrasound guided percutaneous fine needle aspiration is a useful method to obtain specimen for histological diagnosis of peripheral lung lesions. The aim of this study is to evaluate the diagnostic accuracy and complication rate of the procedure performed by pulmonologists. The result is compared with ultrasound guided core needle biopsy performed by radiologists.
Methods:
We retrospectively evaluated the diagnostic accuracy and complication rate of pulmonologist-performed ultrasound guided FNA of lung lesions in the period of 1st August 2019 to 30th June 2021 (pulmonologist group) and radiologist-performed ultrasound guided CNB of lung lesions in the period of 1st January 2010 to 31st December 2014 (radiologist group). Logistic regression analysis was used to identify independent influence factors associated with diagnostic accuracy in pulmonologist group and in combining both groups.
Results:
In a 23-month period, pulmonologists in a tertiary center performed 113 episodes of ultrasound guided fine needle aspiration for peripheral lung lesions. The diagnostic accuracy and complication rate were 80.4% and 5.3% respectively, comparable to 86.8% and 7.4% in a historical cohort consisting of 68 episodes of ultrasound guided core needle biopsy performed by radiologists in the same hospital. Lung lesions of upper lobe location were predictive of successful diagnosis.
Conclusion:
Ultrasound guided fine needle aspiration by pulmonologist is an easily accessible and reliable method to obtain specimen for histological diagnosis.
Humoral immune response to tumor-associated antigen Ubiquilin 1 (UBQLN1) and its tumor-promoting potential in lung cancer
Authors: Wang Yulin , Ouyang Songyun , Liu Man , Si Qiufang , Zhang Xue , Zhang Xiuzhi , Li Jiaqi , Wang Peng , Ye Hua , Shi Jianxing , Song Chunhua , Wang Kaijuan , Dai Liping , Zhang Jianying
Published: November 2022

Background
The aim of this study is to investigate the expression of UBQLN1 in lung cancer (LC) tissue and the diagnostic capability of autoantibody to UBQLN1 (anti-UBQLN1) in the detection of LC, as well as discriminating malignant and benign pulmonary nodules (PNs).
Methods
Sera from 798 participants of three independent cohorts were used to discover and validate the level of autoantibodies via HuProt microarray and Enzyme-linked immunosorbent assay (ELISA). Logistic regression analysis was applied to establish model combining anti-UBQLN1, CT characteristics and traditional serum biomarkers. Receiver operating characteristic curve (ROC) analysis was performed to evaluate the diagnostic potential. Immunohistochemistry of tissue array was performed to detect UBQLN1 expression in 88 LC tissues and 88 para-tumor tissues. qRT-PCR and western blotting were performed to detect the expression of UBQLN1 at the mRNA and protein levels in cell lines, respectively. Trans-well assay and cell counting kit-8 (CCK-8) was used to investigate the functions of UBQLN1 in two lung cancer cell lines (CALU3 and H358).
Results
Anti-UBQLN1 was identified with the highest fold change by means of protein microarray in the discovery cohort. The level of anti-UBQLN1 in LC patients was obviously higher than that in NC or patients with benign lung disease of validation cohort 1 (P < 0.05). The area under the curve (AUC) of anti-UBQLN1 was 0.610 (95%CI: 0.508–0.713) while reached at 0.822 (95%CI: 0.784–0.897) when combining anti-UBQLN1with CEA, CYFRA21-1, CA125 and three CT indicators (vascular notch sign, lobulation sign and mediastinal lymph node enlargement) in the discrimination of malignant from benign PNs. UBQLN1 protein was overexpressed in lung adenocarcinoma (LUAD) tissues compared to para-tumor tissues. UBQLN1 knockdown remarkably inhibited the migration, invasion and proliferation of two lung cancer cell lines.
Conclusions
UBQLN1 can elicit the humoral immune response as tumor-associated antigen in LC. The autoantibody to UBQLN1 might be a potential biomarker for LC diagnosis and might be useful to improve the discrimination of malignant from benign PNs.
Bridging synthesis and controllable doping of monolayer 4-inch transition-metal dichalcogenides single crystals with record-breaking electron mobility
Authors: Li Hui , Yang Junbo , Li Xiaohui , Luo Quankun , Cheng Mo , Feng Wang , Du Ruofan , Wang Yuzhu , Song Luying , Wen Xia , Wen Yao , Xiao Mengmeng , Liao Lei , Zhang Yanfeng , Shi Jianping , He Jun
Published: November 2022

Epitaxial growth and controllable doping of wafer-scale atomically thin semiconductor single crystals are two central tasks to tackle the scaling challenge of transistors. Despite considerable efforts have been devoted, addressing such crucial issues simultaneously under two-dimensional (2D) confinement is yet to be realized. Here we design an ingenious epitaxial strategy to synthesize record-breaking 4-inch Fe-doped transition-metal dichalcogenides (TMDCs) single crystals on industry-compatible c-plane sapphire without miscut angle. Atomically thin transistors with the highest recorded electron mobility (~231 cm2 V−1 s−1) and remarkable on/off current ratio (~109) are fabricated based on 4-inch Fe-MoS2 single crystals, due to the ultralow contact resistance (~489 Ω µm) and subthreshold swing (~95 mV dec−1). In-depth characterizations and theoretical calculations reveal that the introduction of Fe significantly decreases the formation energy of parallel steps on sapphire surfaces and contributes to the edge-nucleation of unidirectional alignment TMDCs domains (>99%), as well as the modulation of band structures. This work represents a substantial leap in terms of bridging synthesis and doping of wafer-scale 2D semiconductor single crystals without the need for substrate miscut, which should promote the further device downscaling and extension of Moore’s law.
Future climate change will decrease the carbon stock in three-quarters of global protected areas
Authors: Shi Hong , Li Xia , Wang Shaojian , Fang Chuanglin , Li Taohong , Liu Xiaojuan , Zhang Han , Zhang Qi , Yan Yuchao , Tang Dongmei , Hubacek Klaus
Published: November 2022

The improvement of the habitat of protected areas is of great importance to meet the Convention on Biological Diversity. Using legal means to protect sensitive areas from human interference such as land use change has been an effective way to maintain ecosystem services. Currently 17% of global land is protected yet less effective in face of anthropogenic climate change; the extent to which these ecosystem services are affected by climate change is less understood. Thus, we created the first dataset of the carbon density index, which represents the amount of carbon stock per unit of protected land, and assessed past and future effects of climate change. We found that future climate change will decrease the carbon stock of 75.0% of global protected areas. Latitudinal differences and the size of protected areas will also affect the vulnerability of protected areas to future climates. The protected areas located between N40–60° will suffer the most from future climates. Connecting small protected areas and upgrading the protection level of such areas can be effective to tackle climate change. We find that small protected areas (less than 1 km2), which account for 37.2% of the total protected area can improve their connectivity for less vulnerability.
Thymoquinone induces G2/M cell cycle phase arrest and apoptosis through inhibition of JNK phosphorylation and induction of p53 and p21 expression in HT-1080 fibrosarcoma cells
Authors: Mahjoub Sana , Dhiflaoui Amani , Almawi Wassim Y. , Mahjoub Touhami , Morjani Hamid , Martiny Laurent , Devarenne-Charpentier Emmanuelle , Btaouri Hassan EL
Published: November 2022

Background
Resistance to chemotherapy is a major cause of failure in cancer treatment. Several approaches have been used to circumvent this resistance, including the co-treatment with ABC proteins inhibitors. However, such strategy did not significantly improve cancer therapy due to toxicity and bioavailability of these compounds. Antitumor activity of natural compounds has been largely explored during the last decades as an alternative to improve cancer treatment. One of explored natural molecules is thymoquinone which has been demonstrated to inhibit proliferation and to induce apoptosis in different tumor cell lines. Thymoquinone is able to activate several cellular pathways and thereby to affect cell proliferation and survival. Methods: The HT1080 human fibrosarcoma cells has been treated with Thymoquinone and JNK inhibitor SP600125.
Results
We showed that thymoquinone arrested cell cycle at the G2M phase and induced apoptosis of HT1080 cells. These effects were mediated through the inhibition of JNK phosphorylation and induction of p53 and p21 expression. The use of the JNK inhibitor SP600125 demonstrated that the inhibition of this pathway is involved in the thymoquinone-induced apoptosis and cell cycle arrest.
Conclusions
Our data clearly showed that thymoquinone, a naturally-occurring compound, induced G2/M cell cycle phase arrest and apoptosis of human fibrosarcoma HT1080 cells via inhibition of JNK phosphorylation and induction of p53 and p21 expression.
Mussel-inspired Injectable Microbubbles Delivery System for Drug Ultrasound Contrast and Long-lasting Analgesic Effect in Peripheral Nerve Block
Authors: Shang Huijie , Ye Haotian , Yue Caibao , Ji Muhuo , Gu Hanwen , Pan Weitong , Liu Panmiao , Yang Jianjun
Published: November 2022

Background
Ultrasound-guided peripheral nerve block is difficult to accurately identify the diffusion location of injected local anesthetics. Moreover, current available local anesthetics are limited in duration, which is inadequate for the treatment of prolonged pain. We designed a drug delivery system by using adhesive polylactic-co-glycolic acid (PLGA) microbubbles loaded with ropivacaine to accurately identify and deliver the local anesthetics to the desired targeted site for prolonged analgesic time in rat pain models.
Methods
The adhesive PLGA-ropivacaine microbubbles (APRMs) were fabricated by coating polydopamine on the PLGA microbubbles with ropivacaine embedded in the shell. Ropivacaine release and ultrasonographic experiments of APRMs were conducted in vitro. Then, incision surgery and SNI-induced neuropathic pain were conducted for adult male rats to verify the ropivacaine release of APRMs in vivo. Ultrasound imaging was performed to confirm the ultrasonic visualization of APRMs. The in vivo fluorescence imaging experiment was conducted for the adhesion property of APRMs. Finally, systemic toxicity and tissue reaction were histologically evaluated.
Results
In vivo, these microbubbles were able to accurately identify and release local anesthetics to targeted sites for prolonged analgesia. Results showed that APRMs not only presented a continuous release of ropivacaine for at least 16 days, but also exhibited strong dispersed echo spots in agarose gel. In animal model studies, APRMs alleviated mechanical allodynia and thermal hyperalgesia in neuropathic and postoperative pain, which lasted at least 7 days. More importantly, the APRMs performed a lower agent spread area and longer analgesic time than PRMs and ropivacaine groups in thermal analgesic test. Additionally, histological systemic toxicity and tissue reactions evaluated that APRMs did not display any detectable systemic toxicity or adverse tissue reactions.
Conclusions
APRMs served as an excellent nerve blocker in this delivery system to achieve accurate, sustainable, and safe analgesic efficacy for pain management.
Vision-based In-Hand Manipulation via Picking up by a Two-fingered Robotic Hand with Conveyor Belts
Authors: Isobe Yuzuka , Pathak Sarthak , Umeda Kazunori , Hashimoto Yusuke , Matsuyama Yoshinari , Matsuda Taku , Kaneda Yu , Ikeuchi Hiroki , Tadakuma Kenjiro
Published: November 2022

This paper presents a vision-based in-hand manipulation method. Picking up an object from a pile is an important task for a robotic hand. In particular, for objects of various sizes, weights, and hardness, it is necessary to detect slippage between the object and the surface of the hand. Meanwhile, dexterously changing the position and orientation of the grasped object in the hand workspace is also necessary for sequential pick-and-place tasks. In an effort to achieve sequential motion, an algorithm for picking up and translating a desired object is proposed. A two-jaw parallel gripper with a conveyor belt on each gripper surface is used, which is capable of both translating and rotating a grasped object. In order to observe the slippage, the actuation of the belts is combined with the detection of the manipulation status from images captured by a stereo camera attached to the hand. With an algorithm for the combination, both picking up and translating various objects of unknown sizes and hardness are achieved without needing machine learning models (which require large amounts of training data) or any kind of tactile sensing. The validity of the proposed method is verified through experiments to pick up various objects and translate them to the given position.
A Tetramethylpyrazine Releasing Hydrogel can Potentiate CAR-T Cell Therapy against Triple negative breast cancer by Reprogramming Tumor Vasculatures
Authors: Liu Yan , Hao Yu , Lv Xiang , Zhang Yefei , Ma Xinxing , Zhou Yehui , Feng Liangzhu
Published: November 2022

Background: Irregular vasculature of solid tumors has proven to be a pivotal factor restricting their response to chimeric antigen receptor-T (CAR-T) cell therapy because it is tightly associated with hypoxia and other biological barriers.
Methods: Herein, an injectable hydrogel composed of poly (ethylene glycol) dimethacrylate (PEGDMA) and ferrous chloride (FeCl2) responding to endogenous hydrogen peroxides (H2O2) is developed to enable sustained intratumoral release of Chinese herbal extracts tetramethylpyrazine (TMP). then the effect and mechanism of angiogenesis and tumor vasculature maturation by TMP@PEGgel were evaluated by immunofluorescence, flow cytometry, western blotting, real-time PCR and enzyme linked immunosorbent assay. Furthermore, tumor infiltration, survival, and effector function of CAR-T cell were investigated by in vitro different cell assay and in vivo tumor xenografts mouse models.
Results: Upon being fixed inside tumors with the PEGDMA based hydrogel, TMP can remodel tumor vasculature by simultaneously promoting angiogenesis and dilating tumor vasculature via activating vascular endothelial growth factor (VEGF) expression and the endothelial nitric oxide synthase/nitric oxide (eNOS/NO) axis inside vascular endothelial cells and thus attenuate tumor hypoxia in two murine xenografts bearing human triple negative breast cancer (TNBC). Resultantly, treatment with TMP fixation potentiate the tumor suppression effect of intravenously injected epidermal growth factor receptor expressing CAR-T (HER-1-CAR-T) cells toward two TNBC tumor xenografts by promoting their tumor infiltration, survival, and effector function.
Conclusion: This study highlights that TME remodeling through reprogramming tumor vasculature with TMP@PEGgel treatment is a promising and robust strategy to reinforce the therapeutic potency of CAR-T cell therapies toward solid tumors.
Prevalence and genotype distribution of human papillomavirus in outpatients and healthy female subjects in Wenzhou, Zhejiang Province, China
Authors: Chen Qiong , Qu Wanglei , Zhao Yu , Shu Li , Wang Yi , Chen Xiangnan
Published: November 2022

The present study examined the prevalence of human papillomavirus (HPV) and its genotype distribution in outpatients and healthy female subjects in Wenzhou so as to develop local HPV vaccination strategies and contribute to the prevention of this disease. The present retrospective study enrolled 164,137 women, including 118,484 outpatients and 45,653 healthy female subjects from 2015 to 2020. Cervical exfoliated cells were collected from these women for DNA extraction. The DNA samples were detected with a fluorescence in situ hybridization method and 27 HPV genotypes were analyzed. The overall prevalence of HPV was 17.35%; this corresponded to a prevalence of 19.10% in the outpatient group and 12.82% in the healthy female group. HPVs 52, 58, 16, 53, and 61 were the five most prevalent HPV genotypes in the outpatient group. The five most common genotypes were HPV 52, 53, 58, 61, and 81 in the healthy female group. The HPV infection peak was estimated to be 44.65% in the 10–19 age group and 27.35% in the 60–69 age group. The burden of HPV infection in this area was high; therefore, a scientific and reasonable vaccination strategy should be adopted. The universal use of multivalent vaccines is recommended and considerable attention should be paid to HPV types that are not targeted by the vaccines. Cervical screening should be performed routinely in patients with gynecological clinical symptoms to avoid cervical intraepithelial neoplasia following persistent infection of high-risk HPV, notably in women over 60 years of age.
Effects of land use on habitat quality based on INVEST model:A Case Study of the Zuli River Basin,china
Authors: Zhang Xiaodong , Liu Xuelu
Published: November 2022

With the development of urbanization,the intensity of human use of land is increasing,which in turn has an impact on habitat quality. Therefore, the study of synergistic changes in land use and habitat quality plays an important role in watershed ecological protection and high-quality development.Based on the data of remote sensing in the five phases of 1980,1990,2000,2010 and 2020,the paper studies land use changes, habitat quality changes and synergistic changes in the Zuli River Basin.(1)The land use type of Zuli River basin is dominated by grassland and arable land, which account for more than 90% of the total area of the basin;(2)The rate of change in watershed land use from 1980 to 2020 is Water > Construction Land > Woodland > Grassland > Arable Land, The rate of increase of water, construction land and forest land was 20.38%,6.97% and 5.23%, respectively.The reduction rates of cultivated land and grassland were 0.27% and 0.2% respectively;(3)land use transfer in the Zuli River Basin was divided into two phases. From 1980 to 2000, with the transfer of arable land and grassland, and the transfer of woodland and construction land, cultivated land and grassland diversions were 12.90km2 and 38.19km2.The transfer volume of woodland and construction land is 16.62 km2 and 24 km2 2000 to 2020 respectively. Grassland, woodland, construction land and water diversion, 96.78 km2;(4)the overall habitat quality of the Zuli River basin increased in 1980–2020.Average habitat quality scores were 0.502, 0.5, 0.49, 0.504 and 0.52 respectively,but from the point of view of the development process were reduced and then increased;(5)There is a synergistic relationship between land use and habitat quality, and the change of grassland area in the basin influences the change of habitat quality,which is expected to provide a basis for ecological protection, land use planning and ecological protection and high-quality development of the Yellow River basin. This study aims to provide a basis for ecological protection, land use planning and ecological protection and quality development of the Yellow River basin.
WITHDRAWN: RNF125 attenuates hepatocellular carcinoma progression by downregulating SRSF1-ERK pathway
Authors: Ma Yong , Feng Zhigang , Ke Shanjia , Wang Chaoqun , Lu Shounan , Xu Yanan , Yu Hongjun , Li Zihao , Yin Bing , Li Xinglong , Hua Yongliang , Qian Baolin , Bai Miaoyu , Fu Yao , Zhang Yingmei , Wu Yaohua
Published: November 2022

Hepatocellular carcinoma (HCC) is one of the most deadly malignant cancers worldwide. Research into the crucial genes responsible for maintaining the aggressive behaviour of cancer cells is important for the clinical treatment of HCC. The purpose of this study was to determine whether the E3 ubiquitin ligase Ring Finger Protein 125 (RNF125) plays a role in the proliferation and metastasis of HCC. RNF125 expression in human HCC samples and cell lines was investigated using TCGA dataset mining, qRT‒PCR, western blot, and immunohistochemistry assays. In addition, 80 patients with HCC were studied for the clinical value of RNF125. Furthermore, the molecular mechanism by which RNF125 contributes to hepatocellular carcinoma progression was determined with mass spectrometry, coimmunoprecipitation (Co-IP), dual-luciferase reporter assays, and ubiquitin ladder assays. We found that RNF125 was markedly downregulated in HCC tumour tissues, which was associated with a poor prognosis for patients with HCC. Moreover, the overexpression of RNF125 inhibited HCC proliferation and metastasis both in vitro and in vivo, whereas the knockdown of RNF125 exerted antithetical effects. Mechanistically, mass spectrometry analysis revealed a protein interaction between RNF125 and SRSF1, and RNF125 accelerated the proteasome-mediated degradation of SRSF1, which impeded HCC progression by inhibiting the ERK signalling pathway. Furthermore, RNF125 was detected to be the downstream target of miR-103a-3p. In this study, we identified that RNF125 is a tumour suppressor in HCC and inhibits HCC progression by inhibiting the SRSF1/ERK pathway. These findings provide a promising treatment target for HCC.
A Comprehensive Comparison of Swarm Optimization-based Extreme Learning Machines to Predict Compression Index of Clay
Authors: Thieu Nguyen Van , Zhou Jian , Romulus Costache , Tran Trung Tin
Published: November 2022

Soft ground improvement is a considerable concern of many researchers worldwide in geotechnical works. In this study, the compressibility of clay (Cc) was considered for compacting the soil for soft ground improvement, and various novel intelligence models have predicted it. Indeed, a dataset containing 739 samples in the laboratory was investigated and used to develop intelligence models for predicting Cc. The extreme learning machine (ELM) was selected for this task. It was then optimized by six metaheuristic algorithms, including particle swarm optimization (PSO), moth search optimization (MSO), firefly optimization (FO), cuckoo search optimization (CSO), bees optimization (BO), and ant colony optimization (ACO), named as PSO-ELM, MSO-ELM, FO-ELM, CSO-ELM, BO-ELM, and ACO-ELM models. We used 517 samples (~ 70%) to develop models and 222 samples (~ 30%) to test the accuracy of those models. The results indicated that the accuracies of hybrid meta-heuristic-based ELM models improved from 3–5% compared to the original ELM model in predicting Cc. The highest accuracy of 87% was also reported in this study with the BO-ELM model when predicting Cc on the testing dataset. It was introduced as a robust model for predicting Cc in practical engineering that can assist in improving the soft ground.
Akkermansia muciniphila alleviates colonic epithelial endoplasmic reticulum stress through activation of farnesoid X receptors in murine experimental colitis
Authors: Bu Fan , Zhou Qiao , Dong Yuwei , Bao Han , Lu Zhihua , Yuan Xiaomin , Chen Chen , Jiang Feng , Tao Yu , Zhang Wei , Zhang Dan , Chen Yugen , Wang Qiong
Published: November 2022

Background
Endoplasmic reticulum (ER) stress-related mucin depletion could be involved in the pathogenesis of inflammatory bowel disease (IBD). Akkermansia muciniphila (A. muciniphila), a symbiotic bacterium of the mucus layer, uses mucin as its sole energy source and shows potential in the treatment of colitis. However, the effects and underlying mechanisms of A. muciniphila on colonic epithelial ER stress in colitis are largely unknown.
Methods
Colitis was induced by adding 2.5% DSS in drinking water. Mice were orally administered A. muciniphila (3*10^7, 3*10^8 cfu/day) once daily for 10 days during DSS intervention. UHPLC high-resolution orbitrap mass spectrometry-based metabolomic analyses were performed on faeces. 16S rRNA sequencing were used to quantify and characterize the gut microbiota of mice and human. Colons were collected from mice and analyzed by histopathology, quantitative PCR and immunofluorescence. Colon biopsies from the patients with ulcerative colitis (UC) and controls were collected and analyzed by immunohistochemistry.
Results
Metabolite pathway enrichment analysis demonstrated that colitis-affected metabolites after A. muciniphila supplementation were mainly enriched in mineral absorption, bile secretion and protein digestion and absorption. P-hydroxyphenyl acetic acid, which showed the highest VIP scores, was significantly increased by A. muciniphila, and could cause ER stress. A. muciniphila supplementation changed the relative abundance and composition of intestinal microbiotaespecially a decrease inParasutterella, which showed the potential role in bile acid maintenance. A. muciniphila supplementation protected colon shortening, histological injury, intestinal inflammation and barrier damage in wild-type (WT) mice but not in farnesoid X receptor-null (FXR−/−) mice. Mechanistically, A. muciniphila supplementation activated FXR/SHP signaling, which directly increased X-box binding protein-1 splicing (XBP1s) and phosphorylated inositol requiring enzyme 1α (p-IRE1α) expression, and in turn formed XBP1s-SHP regulatory loop in response to ER stress. We further showed that the abundance of A. muciniphila in faeces from UC patients positively correlates with p-IRE1α expression.
Conclusions
Our results suggest that A. muciniphila supplementation alleviates DSS-induced colitis involvement of the IRE1α/XBP1 ER stress pathway via FXR/SHP axis activation.
Biochemical response of human endothelial and fibroblast cells to silver nanoparticles
Authors: Palomares Cristofher Victor Vivas , Santos Jennifer Adriane , Barreto Yan Borges , Toma Sergio Hiroshi , Santos Jonnatan Julival , Stephano Marco Antonio , Oliveira Cristiano Luis Pinto , Araki Koiti , Alencar Adriano Mesquita , Bloise Antonio Carlos
Published: November 2022

Nowadays the bactericidal and antimicrobial properties of silver nanoparticles, AgNPs, in addition to their cytotoxic effects, have been explored to properly modulate cell biochemistry processes in order to improve the healing of wounds. Herein we investigate the cytotoxicity and metabolic profiling of two human cell lineages, the fibroblast FN1 and endothelial HUV-EC-C, planning doses of AgNPs and incubation times. Cytotoxicity assays showed consistent decrease in proliferation rates, viable cells number, and average surface areas. Metabolomics based on proton Nuclear Magnetic Resonance was successfully used to obtain quantitative and qualitative changes in metabolic events triggered by silver treatments. The metabolic profiling provided by endo- and exometabolome revealed biochemical changes induced on treated cells compared to controls. Glycolytic pathway is up-regulated due to the elevation in glucose consumption; however, the consequent elevation in pyruvate production seems to be wasted by cells to generate energy by aerobic means that are choosing to oxidize it to acetate. Aminoacid metabolism is down-regulated, signalizing the protein degradation mechanism. Tricarboxylic Acid Cycle is also down-regulated, indicating a starvation situation once succinate was left over in the culture media. Concurrently, the ketogenic pathway is up-regulated due to the excess of acetone. Changes in pyroglutamate metabolism were detected indicating the up-regulation of glutathione biosynthesis used to equilibrate the effects induced by oxidative stress, in accordance with the N-Acetylcysteine finds. Phospholipid metabolism is down-regulated, as revealed by the changes in O-Phosphocholine and Sn-Glycerol-3-PC levels, signaling reduction in the cellular proliferation rates. To the best of our knowledge, this is the first report describing AgNP-induced changes in the UDP-GlcNAc levels, which plays an essential role in modifying nucleocytoplasmic proteins. In summary, AgNPs can induce oxidative stress and cytotoxicity in endothelial and fibroblast cells, influencing their endo- and exometabolome.
Diabetes and periodontitis: Bi-directional association in population-based 15-year retrospective cohorts
Authors: Chien Wu-Chien , Fu Earl , Chung Chi-Hsiang , Cheng Chia-Mao , Tu Hsiao-Pei , Lee Wei-Cheng , Chen Wei-Liang , Shih Kuang-Chung
Published: November 2022

Two-way relationship between periodontitis and diabetes was advocated; however, bidirectional observation in general population is still inconclusive. Using the Taiwan Health Insurance Database (covering over 99% of the entire population),11,011 patients with severe periodontitis were recruited from 2000 to 2015.After matching by age, sex, and index date, 11,011 patients with mild periodontitis and 11,011 non-periodontitis controls were registered. The outcome of T2DM was traced. Conversely, the development of periodontitis was traced in 157,798 patients with T2DM, and 157,798 non-diabetic controls enrolled. The risks of T2DM significantly increased in groups with severe and mild periodontitis, with the adjusted hazard ratio (aHR) and 95% confidence interval (CI) being 1.94 (1.49–2.63, p < 0.01) and 1.72 (1.24–2.52, p < 0.01), respectively. Patients with severe periodontitis had a high risk of having diabetes compared to those with mild periodontitis [aHR, 1.17 (95% CI 1.04–1.26, p < 0.001)]. Conversely, the risk of periodontitis increased significantly in patients with T2DM [1.99 (1.42–2.48, p < 0.01)]. However, the high risk was not observed for the outcome of mild periodontitis [0.97 (0.38–1.57, p = 0.462)]. We, therefore, suggested the bi-direction is between diabetes and severe periodontitis, but not in mild type.
CDK4/6i treatment induces ferroptosis via downregulation of SLC7A11 mediated by SP1 for estrogen receptor positive breast cancers.
Authors: Xiaosong Li , Yingshu Cui , Xu Yuanyuang , Yuanyuan Sun , Liu Xinxin , Junwen Ju , Shan Long , Xiaofeng Kang , Zhijia Sun , Yimeng Du , Xu Xiaojie
Published: November 2022

Purpose: Cyclin-dependent kinase 4 and 6 inhibitors (CDK4/6is) can significantly extend tumor response in patients with metastatic estrogen receptor–positive (ER+) breast cancer, but intrinsic and acquired resistance is common. Elucidation of the molecular features of CDK4/6i sensitivity and the efficiency of their combination with novel targeted cell death inducers may pave the way toward improving patient outcomes.
Experimental Design: Ferroptosis-related characteristics were observed following treatment with the CDK4/6 inhibitor palbociclib. Transcriptomic analyses and functional assays were performed to determine the targets and regulatory mechanism of palbociclib in the ferroptosis pathway in ER+ breast cancer cell lines. A tumor xenograft model was used to study the synergistic antitumor effects of CDK4/6is in combination with ferroptosis inducers.
Results: Ferroptosis, a form of regulated cell death driven by iron-dependent phospholipid peroxidation, is partly responsible for the efficacy of the CDK4/6 inhibitor palbociclib. Mechanistically, palbociclib downregulates cystine transporter SLC7A11 by inhibiting SP1 binding to the promoter region of SLC7A11. Furthermore, genetic or pharmacological inhibitors of SP1 or SLC7A11 can enhance cell sensitivity to palbociclib and synergistically inhibit ER+ breast cancer cell growth in combination with palbociclib. A syngeneic ER+ mouse mammary tumor model was used to verify that combined inhibition of SLC7A11 or SP1 and CDK4/6 resulted in marked suppression of tumor growth in vivo.
Conclusions: Experimentally, ferroptosis represents some of the CDK4/6i-induced cell death response. This study illustrates the potential for targeting SLC7A11 in combination with CDK4/6 inhibitors and supports the investigation of combination therapy in ER+ breast cancer.
3,4-benzo[a]pyrene aggravates myocardial infarction injury by activating NLRP3-related pyroptosis through PINK1/Parkin-mitophagy-mPTP opening axis
Authors: Wu Bo-sen , Xiang Hua-qiang , Yu Yong-wei , Liu Shuai , Song Dong-yan , Lin Zhi-hui , Zhu Chen-xi , Wu Chang , Xue Yang-jing , Ji Kang-ting
Published: November 2022

Acute myocardial infarction(AMI) accounts for more than one third of ischemic heart disease deaths. Air pollution exposure,even for a short-term exposure,is conspicuously relevant to increased risk of MI mortality and clinical evidence has showed that air pollution particulate matter(PM) induces the aggravation of AMI.3,4-benzo[a]pyrene(BaP),a polycyclic aromatic hydrocarbon(PAH) with toxicity,is a typical air pollutant present in PM and is often measured as the representative of PAHs.The purpose of this study was to investigate whether BaP can aggravate myocardial infarction(MI) injury and,on this basis,to investigate the relevant mechanisms.The MI mouse model and the oxygen and glucose deprivation(OGD) H9C2 cell model were used to investigate the effect of BaP in MI injury.The involvement of mitophagy and NLRP3-related pyroptosis in regulating deterioration of cardiac function and aggravation of MI injury induced by BaP was comprehensively evaluated.Our study showed that BaP can aggravate MI injury in vivo and in vitro,and this result was based on NLRP3-related pyroptosis induced by BaP.In addition,BaP can inhibit PINK1/Parkin dependent mitophagy through the aryl hydrocarbon receptor(AhR),thus inducing mitochondrial permeability transition pore(mPTP) opening.Our results suggested a role for the BaP from air pollution in the aggravation of MI injury and revealed that BaP aggravates MI injury by activating NLRP3-related pyroptosis through PINK1/Parkin-mitophagy-mPTP opening axis.
Super-strong and flexible wood through cell wall swelling-assisted spontaneously fibers aggregation
Authors: Zhang tao , Chen weimin , Zhang daotong , Chen Yan , Yang kai , Yang pei , Pan nana , Quan qi , Li zhao , Zhou Ke , Chen minzhi , Zhou xiaoyan
Published: November 2022

The highly oriented cellulose microfibrils in secondary cell wall provide a unique advantage for developing super-strength wood. Here, we developed a two-tiered dynamic strategy that realized the self-densified wood by adequate swelling and moisture evaporation process without further hot pressing or via chemical oxidation pre-treatment. Our method requires only exposing delignified wood to a poor solvent that is able to infiltrate the cellulose skeleton without molecular-level dissolution, thus facilitating the effective penetration of moisture upon water replacement. The following natural moisture evaporation triggers self-aggregation of cellulose fibrils accompanying with dynamic re-formation of hydrogen bonds, thereby leading to a super-high mechanical strength (tensile strength: 596.24 ± 57.01 MPa, toughness: 10.43 ± 2.07 MJ m−3, and flexural strength: 418.51 ± 23.86 MPa (balsa wood), 296.52 ± 10.41 MPa (pine wood)) to the resulting self-densified wood. More importantly, the swollen wood has the hydroplastic processing ability (using air-drying to design the shapes). The “cold” processing method satisfies the self-densification for various species of wood, providing a general strengthening strategy.