bluuebunny/crossref_metadata_2025 huggingface dataset:
What about sex, race(ism), and social determinants of health in neonatal outcomes?
Authors: Chaudhary Neha , Meharwal Arushi
Published: October 2024

Neonatal outcomes encompass a range of outcome measures, including mortality rates, physical and mental health morbidities, and long-term neurodevelopmental statistics. These outcomes are influenced by non-modifiable factors, such as sex and race, and modifiable factors, such as social determinants of health and racism. There is a known bias toward worse outcomes for male infants in terms of preterm birth, low birth weight, and mortality, with several biological and physiological factors contributing to these sex-related differences. In relation to racial disparities, wherein race is a social construct, maternal and infant healthcare continues to lag behind for minority populations compared with the white population, despite advances in medical care. Infants born to Black women have higher infant mortality rates and lower birth weights than infants of white women. These differences can be largely attributed to social and environmental factors, rather than racial and ethnic differences. Furthermore, we emphasize the role of social determinants of health in neonatal outcomes. Factors such as economic stability, education access and quality, healthcare access and quality, the physical neighborhood environment, and the social and community context all contribute to these outcomes. Overall, this article highlights the complex interactions between sex, race(ism), and social determinants of health in neonatal outcomes. It underscores the need for a comprehensive understanding of these factors to improve maternal–neonatal care and reduce disparities in outcomes. Healthcare providers, policymakers, and communities need to work together to combat these complex issues and improve neonatal outcomes for all infants, while understanding the complex interplay between sex, racism, and/or social determinants of health.
Policy and practice review consensus statements and clinical guidelines on managing pediatric trauma and orthopedics during the COVID-19 pandemic: a systematic review on the global response for future pandemics and public health crises
Authors: Sugand Kapil , Park Chang , Aframian Arash , Gupte Chinmay M. , Sarraf Khaled M. ,   COVid Emergency Related Trauma and orthopaedics (COVERT) Collaborative
Published: October 2024

Introduction

The COVID-19 pandemic has been recognized as an unprecedented global health crisis. Over 7 million mortalities have been documented with many paediatric fatalities. Trauma and orthopaedic care, much like other specialities, were marginalized due to resource allocation during the pandemic which affected paediatric care. This is the first systematic review to centralise and compile the recommended published guidelines from professional bodies in principally English speaking countries on managing paediatric trauma and orthopaedic care. These guidelines will be required to be implemented sooner and more effectively in case of future pandemics with similar impact.

Methods

A search was conducted on PubMed/MedLine, Cochrane Library and Embase using terms including p(a)ediatric or child* and/or COVID* or coronavirus or SARS-CoV-2 and/or trauma and/or orthop(a)edic* with a simplified MeSH heading [mh] in order to make the search as comprehensive as possible. General terminology was utilized to make the search as exhaustive as possible for this systematic review. Another search was conducted on resources available in the public domain from professional bodies publishing on consensus statements and clinical practice guidelines in countries where English is the principal language managing pediatric trauma and orthopedics. The review adhered to PRISMA guidance.

Results

The search revealed a total of 62 results from both databases and professional bodies. Duplicates were removed. This was then reviewed to identify a total of 21 results which fit the inclusion criteria and included within the main analysis. The guidelines from professional bodies were outlined and categorized into aspects of clinical care.

Discussion

The impact of COVID-19 pandemic has compelled for changes in clinical practice and pediatric management. The systematic review highlights the relevant guidelines on service provision for pediatric patients including indications for urgent referrals, surgical prioritization, reasons for follow-up and trauma guidelines. The rationale for care during the unpredictable evolution of the COVID-19 pandemic may have the potential to be translated and replicated in future pandemics of similar significance.
Unraveling the genetic basis of oil quality in olives: a comparative transcriptome analysis
Authors: Asadi AliAkbar , Tavakol Elahe , Shariati Vahid , Hosseini Mazinani Mehdi
Published: October 2024

Introduction

The balanced fatty acid profile of olive oil not only enhances its stability but also contributes to its positive effects on health, making it a valuable dietary choice. Olive oil's high content of unsaturated fatty acids and low content of saturated fatty acids contribute to its beneficial effects on cardiovascular diseases and cancer. The quantities of these fatty acids in olive oil may fluctuate due to various factors, with genotype being a crucial determinant of the oil's quality.

Methods

This study investigated the genetic basis of oil quality by comparing the transcriptome of two Iranian cultivars with contrasting oil profiles: Mari, known for its high oleic acid content, and Shengeh, characterized by high linoleic acid at Jaén index four.

Results and discussion

Gas chromatography confirmed a significant difference in fatty acid composition between the two cultivars. Mari exhibited significantly higher oleic acid content (78.48%) compared to Shengeh (48.05%), while linoleic acid content was significantly lower in Mari (4.76%) than in Shengeh (26.69%). Using RNA sequencing at Jaén index four, we analyzed genes involved in fatty acid biosynthesis. Differential expression analysis identified 2775 genes showing statistically significant differences between the cultivars. Investigating these genes across nine fundamental pathways involved in oil quality led to the identification of 25 effective genes. Further analysis revealed 78 transcription factors and 95 transcription binding sites involved in oil quality, with BPC6 and RGA emerging as unique factors. This research provides a comprehensive understanding of the genetic and molecular mechanisms underlying oil quality in olive cultivars. The findings have practical implications for olive breeders and producers, potentially streamlining cultivar selection processes and contributing to the production of high-quality olive oil.
Prior brief meditation reduces distractor inhibition during cognitive interference
Authors: Fujino Masahiro , Ooishi Yuuki , Ueda Yoshiyuki , Kitagawa Norimichi , Nomura Michio
Published: October 2024

Background

Mindfulness meditation, comprising focused attention and open monitoring meditations, has been shown to enhance performance on cognitive interference tasks. While this enhancement has been considered not to result from distractor inhibition, no empirical evidence has been provided through behavioral data. In this study, we investigated whether 30-min interventions of focused attention and open monitoring meditations could reduce distractor inhibition in 72 meditation-naïve participants divided into focused attention meditation, open monitoring meditation, and control groups.

Methods

We employed a task set that combined a cognitive interference task with subsequent preference judgment and surprise recognition tasks, utilizing the mere exposure effect paradigm, along with state and trait questionnaires. The mere exposure effect shows that repeated exposure to face images increases one’s preference for them. However, this effect is reduced if participants consciously or unconsciously try to inhibit the face images during stimulus processing. In the cognitive interference task, they judged the direction of the letter superimposed on a distractor face image. In the subsequent preference judgment task, they were asked to rate the preference of face images, half of which were presented in the interference task and the remaining half were not presented. We hypothesized that inhibiting face images presented as distractors would lead to a decrease in preference for them.

Results and discussions

We found that the mere exposure effect was observed in focused attention meditation and open monitoring meditation groups but not in the control group, indicating that compared to the control, focused attention and open monitoring meditations reduce inhibition processes for distractors during cognitive interference tasks. Furthermore, we found a positive correlation between the intensity of the mere exposure effect and state relaxation before the cognitive interference task as well as a negative correlation between the intensity of the mere exposure effect and state anxiety in the focused attention meditation group, but not in the open monitoring meditation group. This suggests that the processes of reducing inhibition in focused attention and open monitoring meditations differ. Our findings contribute to understanding the attentional mechanisms underlying mindfulness meditation during cognitive interference.
Revisiting the musical reminiscence bump: insights from neurocognitive and social brain development in adolescence
Authors: Kudaravalli Rishitha , Kathios Nicholas , Loui Psyche , Davidow Juliet Y.
Published: October 2024

Music listening is enjoyed across the lifespan and around the world. This has spurred many theories on the evolutionary purpose of music. The Music for Social Bonding hypothesis posits that the human capacity to make music evolved for the purpose of creating and preserving relationships between one another. Considering different time periods of music use across the lifespan, adolescence is especially a period of social reorientation away from family towards peers, characterized by new social bonds and increased prosocial behavior. This shift is accompanied by notable structural and functional changes in brain networks supporting reward processing and prosocial behavior. Reviewing the extant literature on developmental cognitive neuroscience and adolescent music use, we propose that neurocognitive changes in the reward system make adolescence an ideal developmental time window for investigating interactions between prosocial behavior and reward processing, as adolescence constitutes a time of relative increase in music reward valuation. Testing this hypothesis may clarify our understanding of developmental trajectories in music reward valuation, and offer insights into why music from adults’ adolescence holds a great deal of personal significance.
Inequalities changes in health services utilization among middle-aged and older adult disabled people in China: based on CHARLS 2011–2018
Authors: Jin Shengxuan , Fa Ruobing , Wu Jiaqi , Lin Jiawei , Zhang Shuyuan , Ali Majid , Chen Shaofan , Qian Dongfu
Published: October 2024

Background

Multiple intersections, including socioeconomic inequalities, influence health equity for disabled people and sub-populations. However, this association has not been sufficiently analyzed among Chinese-impaired persons. This study aimed to investigate the health services utilization and inequalities in middle and older adult persons with disabilities and subgroups.

Methods

The China Health and Retirement Longitudinal Study (CHARLS) database in 2011, 2013, 2015, and 2018 were used. Health services utilization was measured by outpatient, inpatient, and self-treatment service utilization. Types of disabilities were classified into six categories. The pooled cross-section regression, concentration index, horizontal inequity index, and concentration index decomposition were used to evaluate inequalities and explore their main contributing factor.

Results

The utilization and non-utilization of healthcare services showed variations across years (p < 0.05). The CIs and HIs for inpatient health service utilization were positive for all years and disability types. The total CIs of inpatient utilization were the highest (0.248). The highest disparities in utilization of inpatient services were for physical disabilities (0.4515 for CI in 2011), and the highest in self-treatment services were for intellectual disability (0.1538 for CI in 2011). The expenditure factor was the main contributor to inequalities. Chronic disease, educational level, and health insurance factors also contribute to the utilization inequalities.

Conclusion

Policies should promote medical insurance and assistance for disabled people with serious impairment and poor. It is crucial to improve the provision of basic medical services, including meeting the demand for varied disabilities and the accessibility of facilities and equipment to enhance the access and well-being of people with disabilities.
Differentiation of Alzheimer's disease from other neurodegenerative disorders using chemiluminescence immunoassays measuring cerebrospinal fluid biomarkers
Authors: Arendt Philipp , Römpler Katharina , Brix Britta , Borchardt-Lohölter Viola , Busse Mandy , Busse Stefan
Published: October 2024

Introduction

Prior research identified four neurochemical cerebrospinal fluid (CSF) biomarkers, Aβ1–42, Aβ1–40, tTau, and pTau(181), as core diagnostic markers for Alzheimer's disease (AD). Determination of AD biomarkers using immunoassays can support differential diagnosis of AD vs. several neuropsychiatric disorders, which is important because the respective treatment regimens differ. Results of biomarker determination can be classified according to the Amyloid/Tau/Neurodegeneration (ATN) system into profiles. Less is known about the clinical performance of chemiluminescence immunoassays (ChLIA) measuring specific biomarkers in CSF samples from patients suffering from neuropsychiatric impairments with various underlying causes.

Methods

Chemiluminescence immunoassays (ChLIAs, EUROIMMUN) were used to determine Beta-Amyloid (1–40), Beta-Amyloid (1–42), Total-Tau, and pTau(181) concentrations in precharacterized cerebrospinal fluid (CSF) samples from 219 AD patients, 74 patients with mild cognitive impairment (MCI), and 220 disease control (DC) patients.

Results

83.0% of AD patients had ATN profiles consistent with AD, whereas 85.5% of DC patients and 77.0% of MCI patients had profiles inconsistent with AD. AD patients showed significantly lower amyloid ratio Aβ1–42/Aβ1–40 (mean: 0.07) and significantly higher concentrations of tTau (mean: 901.6 pg/ml) and pTau(181) (mean: 129 pg/ml) compared to DC and MCI patients (all p values < 0.0071).

Discussion

The ChLIAs effectively determined specific biomarkers and can support differential diagnostics of AD. Their quality was demonstrated in samples from 513 patients with cognitive impairments, representing a realistic mix of underlying causes for seeking treatment at a memory clinic.
Corporate farming model for sustainable supply chain crude palm oil of independent smallholder farmers
Authors: Witjaksono Julian , Djaenudin Deden , Fery Purba Samuel , Yulianti Astrina , Fadwiwati Andi Yulyani , Muslimin  , Sitompul Rislima Febriani , Azahari Delima Hasri , Imran  , Purba Resmayeti , Seerasarn Nareerut
Published: October 2024

Independent oil palm smallholders in Indonesia contributed significantly to the sustainable of Crude Palm Oil (CPO) supply chain. On the other hand, sustainability in the process of implementing the supply chain system for independent smallholders is an issue that is extremely important for the success of the supply chain system for the CPO company. This study’s objective is to develop an alternative business model with the goal of strengthening the interaction that already exists between independent farmers and cooperatives in ensuring the existence of a supply chain for raw materials and having access to financial resources, knowledge, and technology. The selection of the research location considered several factors, including the size of the area’s oil palm plantations, the number of oil palm farmers who are self-sufficient, and the possibility of locating CPO plant. Study findings that, the new model by business canvas analysis as the upgraded model of existing model business recommended that it should be built based on the cooperative palm oil family which provides farmers with access to financial, replanting, intensification, and extensification and infrastructure by APKASINDO, BPDPKS, Bank, and other stakeholders to sustain the FFB supply chain in cooperation through a partnership program in a long-term scheme. This whole system was developed by corporate farming system based on the cooperative as the off taker of FFB farmers and develop mini plant CPO to produce cooking oil and biofuels and by product as the waste management system as the value added for farmers.
Isolation-protocol, characterization, and in-vitro performance of equine umbilical vein endothelial cells
Authors: Lessiak Ulrike , Melchert Maria , Walter Ingrid , Kummer Stefan , Nell Barbara , Tschulenk Waltraud , Pratscher Barbara
Published: October 2024

Angiogenesis plays a crucial role in various physiological and pathological conditions. However, research in equine angiogenesis is relative limited, necessitating the development of suitable in-vitro models. To effectively analyze angiogenesis in-vitro, it is essential to target the specific cells responsible for this process, namely endothelial cells. Human umbilical vein endothelial cells (HUVECs) are one of the most used in vitro models for studying angiogenesis in humans. Serving as an equivalent to HUVECs, we present a comprehensive isolation protocol for equine umbilical vein endothelial cells (EqUVECs) with relatively minimal requirements, thereby enhancing accessibility for researchers. Umbilical cords obtained from five foals were used to isolate endothelial cells, followed by morphological and immunohistochemical identification. Performance of the cells in various assays commonly used in angiogenesis research was studied. Additionally, EqUVEC expression of vascular endothelial growth factor (VEGF) was assessed using ELISA. EqUVECs exhibited endothelial characteristics, forming a homogeneous monolayer with distinctive morphology. Immunohistochemical staining confirmed positive expression of key endothelial markers including von Willebrand factor (vWF), CD31, and vascular endothelial growth factor receptor-2 (VEGFR-2). Furthermore, performance assessments in in-vitro assays demonstrated the viability, proliferation, migration, tube formation and VEGF-expression capabilities of EqUVECs. The findings suggest that EqUVECs are a promising in-vitro model for studying equine angiogenesis, offering a foundation for further investigations into equine-specific vascular processes and therapeutic interventions.
Integrated metagenomics and metabolomics analyses revealed biomarkers in β-casein A2A2-type cows
Authors: Zhao Jinyan , Wang Chuanchuan , Hu Jiahuan , Ma Ruoshuang , Yu Baojun , Zhao Wei , Wang Hua , Gu Yaling , Zhang Juan
Published: October 2024

In Holstein cows, β-casein, one of the most critical proteins in milk, exists in two main genotypes, A1 and A2. Herein, 45 Holstein cows [categorized into three groups based on β-casein A1A1, A1A2, and A2A2 genotypes (N = 15)] with the same feeding management and litter size were enrolled to explore differences in rumen microflora and metabolites across various β-casein genotypes. Rumen fluids were collected for metagenomics and metabolomics analyses. Metabolomics and weighted gene co-expression network analysis (WGCNA) revealed that arachidonic acid (AA), adrenic acid (AdA), glycocholic acid (GCA), and taurocholic acid (TCA) were significantly and positively correlated with milk fat % in dairy cows (p < 0.05). Furthermore, macro-genomics and Spearman’s correlation analysis revealed significant positive correlations (p < 0.05) between the characteristic flora (g_Acetobacter, g_Pseudoxanthomonas, g_Streptococcus, and g_Pediococcus) and the five characteristic metabolites in the rumen of A2A2 dairy cows. Moreover, functional enrichment analysis revealed more genes enriched to the TRP channel’s inflammatory mediator-regulated pathway and the mTOR signaling pathway in A2A2 genotyped cows. Additionally, the regulatory effects of AA on bovine mammary epithelial cells (BMECs) were examined using CCK-8, EdU, and qRT-PCR assays, revealing that AA promoted triglyceride (TG) synthesis and upregulated the milk fat marker genes including SREBF1, ACSS2, AGPAT6, and FASN. Overall, we identified characteristic microorganisms and metabolites in A2A2 Holstein cows and established that AA could be a biomarker for higher milk fat %.
Predictors of Overweight/Obesity Among Preschool Children in Arba Minch City, Southern Ethiopia: Does Consumption of Sweet Food Predispose to Overweight/Obesity?
Authors: Wagaye Kefene Simegn , Guyo Tamirat Gezahegn , Assefa Darik Temesgen , Chisha Yilma , Sorrie Muluken Bekele
Published: October 2024

Objectives

Childhood overweight/obesity is a serious public health problem in the world today specially low-and-middle income countries like Ethiopia. This study aimed to assess the prevalence of overweight/obesity and associated factors among preschool children.

Methods

A community-based cross-sectional study was conducted among preschool children aged 3–5 years in Arba Minch city from April to June 2022. A multistage sampling method was used to select 577 study participants. Data were collected using a structured and pretested questionnaire. A binary logistic regression model was used for analysis. An adjusted odds ratio (AOR) with a 95% confidence interval (CI) was used to assess the strength of the association.

Results

The combined prevalence of overweight/obesity was 23.9% (95% CI: 20.5, 27.6). Age of 36–47 months, family size < five, watching TV/video for ≥2 h, and consuming sweet foods, were factors associated with being overweight/obese among preschool children.

Conclusion

Overweight/obesity was predicted by consuming sweet foods, long time TV/video watching, and large family size in the study area. Special attention should be given to child feeding practices and reducing sedentary lifestyles.
Early Detection of Residual/Recurrent Lung Malignancies on Post-Radiation FDG PET/CT
Authors: Chen Liyuan , Lowe Avanka , Wang Jing
Published: October 2024

Positron Emission Tomography/Computed Tomography (PET/CT) using Fluorodeoxyglucose (FDG) is an important imaging modality for assessing treatment outcomes in patients with pulmonary malignant neoplasms undergoing radiation therapy. However, distinguishing between benign post-radiation changes and residual or recurrent malignancies on PET/CT images is challenging. Leveraging the potential of artificial intelligence (AI), we aimed to develop a hybrid fusion model integrating radiomics and Convolutional Neural Network (CNN) architectures to improve differentiation between benign post-radiation changes and residual or recurrent malignancies on PET/CT images. We retrospectively collected post-radiation PET/CTs with identified labels for benign changes or residual/recurrent malignant lesions from 95 lung cancer patients who received radiation therapy. Firstly, we developed separate radiomics and CNN models using handcrafted and self-learning features, respectively. Then, to build a more reliable model, we fused the probabilities from the two models through an evidential reasoning approach to derive the final prediction probability. Five-folder cross-validation was performed to evaluate the proposed radiomics, CNN, and fusion models. Overall, the hybrid fusion model outperformed the other two models in terms of sensitivity, specificity, accuracy, and the area under the curve (AUC) with values of 0.67, 0.72, 0.69, and 0.72, respectively. Evaluation results on the three AI models we developed suggest that handcrafted features and learned features may provide complementary information for residual or recurrent malignancy identification in PET/CT.
Adaptive Compensation for Robotic Joint Failures Using Partially Observable Reinforcement Learning
Authors: Pham Tan-Hanh , Aikins Godwyll , Truong Tri , Nguyen Kim-Doang
Published: October 2024

Robotic manipulators are widely used in various industries for complex and repetitive tasks. However, they remain vulnerable to unexpected hardware failures. In this study, we address the challenge of enabling a robotic manipulator to complete tasks despite joint malfunctions. Specifically, we develop a reinforcement learning (RL) framework to adaptively compensate for a nonfunctional joint during task execution. Our experimental platform is the Franka robot with seven degrees of freedom (DOFs). We formulate the problem as a partially observable Markov decision process (POMDP), where the robot is trained under various joint failure conditions and tested in both seen and unseen scenarios. We consider scenarios where a joint is permanently broken and where it functions intermittently. Additionally, we demonstrate the effectiveness of our approach by comparing it with traditional inverse kinematics-based control methods. The results show that the RL algorithm enables the robot to successfully complete tasks even with joint failures, achieving a high success rate with an average rate of 93.6%. This showcases its robustness and adaptability. Our findings highlight the potential of RL to enhance the resilience and reliability of robotic systems, making them better suited for unpredictable environments.
Theoretical Study of the Wear of a Reduced-Diameter Wheel for Freight Wagons, Based on Its Diameter
Authors: Pellicer David S. , Larrodé Emilio
Published: October 2024

This paper presents the development of a numerical analysis model designed to estimate the lifespan of reduced-diameter wheels used in freight wagons based on their diameter, under quasi-static conditions. These wheels are increasingly being used in combined transport applications, where they are installed in various bogie configurations and subjected to different operational environments. However, due to the unique characteristics of reduced-diameter wheels, their lifespan has been scarcely studied. To accurately build this model, an in-depth investigation of the rolling phenomenon was required, addressing key issues in the track–vehicle interaction and establishing relationships between these factors. After constructing the rail–wheel interaction model, it was applied to calculate the lifespan of wheels with standard, medium, and reduced diameters under identical conditions for comparison. This approach makes it possible to determine the lifespan of reduced-diameter wheels relative to standard ones, as well as to observe how lifespan changes with wheel diameter, and it is observed how lifespan diminishes non-linearly with decreasing diameters. The underlying reasons for this variation are explained through a comprehensive understanding of the rolling phenomenon, enabled by the full analysis.
Solving Optimal Power Flow Using New Efficient Hybrid Jellyfish Search and Moth Flame Optimization Algorithms
Authors: Mayouf Chiva , Salhi Ahmed , Haidara Fanta , Aroua Fatima Zahra , El-Sehiemy Ragab A. , Naimi Djemai , Aya Chouaib , Kane Cheikh Sidi Ethmane
Published: October 2024

This paper presents a new optimization technique based on the hybridization of two meta-heuristic methods, Jellyfish Search (JS) and Moth Flame Optimizer (MFO), to solve the Optimal Power Flow (OPF) problem. The JS algorithm offers good exploration capacity but lacks performance in its exploitation mechanism. To improve its efficiency, we combined it with the Moth Flame Optimizer, which has proven its ability to exploit good solutions in the search area. This hybrid algorithm combines the advantages of both algorithms. The performance and precision of the hybrid optimization approach (JS-MFO) were investigated by minimizing well-known mathematical benchmark functions and by solving the complex OPF problem. The OPF problem was solved by optimizing non-convex objective functions such as total fuel cost, total active transmission losses, total gas emission, total voltage deviation, and the voltage stability index. Two test systems, the IEEE 30-bus network and the Mauritanian RIM 27-bus transmission network, were considered for implementing the JS-MFO approach. Experimental tests of the JS, MFO, and JS-MFO algorithms on eight well-known benchmark functions, the IEEE 30-bus, and the Mauritanian RIM 27-bus system were conducted. For the IEEE 30-bus test system, the proposed hybrid approach provides a percent cost saving of 11.4028%, a percent gas emission reduction of 14.38%, and a percent loss saving of 50.60% with respect to the base case. For the RIM 27-bus system, JS-MFO achieved a loss percent saving of 50.67% and percent voltage reduction of 62.44% with reference to the base case. The simulation results using JS-MFO and obtained with the MATLAB 2009b software were compared with those of JS, MFO, and other well-known meta-heuristics cited in the literature. The comparison report proves the superiority of the JS-MFO method over JS, MFO, and other competing meta-heuristics in solving difficult OPF problems.
Impact of Sliding Window Variation and Neuronal Time Constants on Acoustic Anomaly Detection Using Recurrent Spiking Neural Networks in Automotive Environment
Authors: Kshirasagar Shreya , Guntoro Andre , Mayr Christian
Published: October 2024

Acoustic perception of the automotive environment has the potential to advance driving potentials with enhanced safety. The challenge arises when these acoustic perception systems need to perform under resource and power constraints on edge devices. Neuromorphic computing has introduced spiking neural networks in the context of ultra-low power sensory edge devices. Spiking architectures leverage biological plausibility to achieve computational capabilities, accurate performance, and great compatibility with neuromorphic hardware. In this work, we explore the depths of spiking neurons and feature components with the acoustic scene analysis task for siren sounds. This research work aims to address the qualitative analysis of sliding windows’ variation on the feature extraction front of the preprocessing pipeline. Optimization of the parameters to exploit the feature extraction stage facilitates the advancement of the performance of the acoustics anomaly detection task. We exploit the parameters for mel spectrogram features and FFT calculations, prone to be suitable for computations in hardware. We conduct experiments with different window sizes and the overlapping ratio within the windows. We present our results for performance measures like accuracy and onset latency to provide an insight on the choice of optimal window. The non-trivial motivation of this research is to understand the effect of encoding behavior of spiking neurons with different windows. We further investigate the heterogeneous nature of membrane and synaptic time constants and their impact on the accuracy of anomaly detection. On a large scale audio dataset comprising of siren sounds and road traffic noises, we obtain accurate predictions of siren sounds using a recurrent spiking neural network. The baseline dataset comprising siren and noise sequences is enriched with a bird dataset to evaluate the model with unseen samples.
Design and Test of Potato Seedling Killing and Residual Film Recycling Integrated Machine
Authors: Chen Yangzhou , Xing Ruofei , Liu Xiaolong , Zhang Hua , Li Hui
Published: October 2024

Plastic film mulching technology can effectively enhance crop yield and quality, and the use of mulch has been increasing in recent years; however, the problem of mulch residue is worsening due to the large amount of recycling work and slow natural degradation. In this study, a potato seedling killing and residual film recycling machine is designed to provide good working conditions for potato harvesters before harvesting in response to the problems of difficult separation of film tangles, the low net rate of recycling due to the mixing of residual film with soil, and the high soil content in residual film recycling operations in northwest China. The machine is based on the potato monoculture and double row planting mode in Gansu area. This paper puts forward the overall design scheme and carries out the theoretical analysis and parameter determination of the key components, such as the seedling killing device, the film surface cleaning device, the film unloading device, and so on. Using EDEM software to carry out the virtual simulation test and Design-Expert13 to analyze the test results, we determined the optimal working scheme for the machine, with a forward speed of 0.8 m/s, a film gap of 125 mm, and a spiral stirrer speed of 600 r/min. Based on a field test for verification, the test results show that the machine’s residual film recovery rate was 83.3%, the impurity rate was 3.8%, and the rate of injury to the potatoes was 1.4%. The machine meets the requirements of national and industry standards, and it can simultaneously realize straw crushing, film surface cleaning, residual film recycling, and hydraulic film unloading operations, with better operating results and while reaching the expected results. It can also provide a reference for the design and testing of a seeding and residual film recycling machine.
Operando Fabricated Quasi-Solid-State Electrolyte Hinders Polysulfide Shuttles in an Advanced Li-S Battery
Authors: Das Sayan , Gupta Krish Naresh , Choi Austin , Pol Vilas
Published: October 2024

Lithium-sulfur (Li-S) batteries are a promising option for energy storage due to their theoretical high energy density and the use of abundant, low-cost sulfur cathodes. Nevertheless, several obstacles remain, including the dissolution of lithium polysulfides (LiPS) into the electrolyte and a restricted operational temperature range. This manuscript presents a promising approach to addressing these challenges. The manuscript describes a straightforward and scalable in situ thermal polymerization method for synthesizing a quasi-solid-state electrolyte (QSE) by gelling pentaerythritol tetraacrylate (PETEA), azobisisobutyronitrile (AIBN), and a dual salt lithium bis(trifluoromethanesulfonyl)imide (LiTFSI) and lithium nitrate (LiNO3)-based liquid electrolyte. The resulting freestanding quasi-solid-state electrolyte (QSE) effectively inhibits the polysulfide shuttle effect across a wider temperature range of −25 °C to 45 °C. The electrolyte’s ability to prevent LiPS migration and cluster formation has been corroborated by scanning electron microscopy (SEM) and Raman spectroscopy analyses. The optimized QSE composition appears to act as a physical barrier, thereby significantly improving battery performance. Notably, the capacity retention has been demonstrated to reach 95% after 100 cycles at a 2C rate. Furthermore, the simple and scalable synthesis process paves the way for the potential commercialization of this technology.
Common Variants in the TYR Gene with Unclear Pathogenicity as the Cause of Oculocutaneous Albinism in a Cohort of Russian Patients
Authors: Shchagina Olga , Stepanova Anna , Mishakova Polina , Kadyshev Vitaliy , Demina Nina , Bessonova Ludmila , Ionova Sofya , Guseva Daria , Marakhonov Andrey , Zinchenko Rena , Kutsev Sergey , Polyakov Aleksander
Published: October 2024

Background: oculocutaneous albinism (OCA) is a hereditary impairment of skin, hair, and eye pigmentation. The most common form of albinism is autosomal recessive albinism, caused by mutations in the TYR gene, accounting for approximately 40–50% of all cases of the disease in European populations. Common hypomorphic variants in the TYR gene could lead to a mild form of albinism in a compound heterozygous state with a pathogenic variant. Methods: we examined by allele specific MLPA a cohort consisting of 118 unrelated patients with albinism and 10 parents of these patients. The control cohort consisted of 200 unexamined Russian residents. Results: the patients with albinism were divided into three groups: without pathogenic variants in the TYR gene—70 patients, with one pathogenic variant in the TYR gene—20 patients, and with two pathogenic variants in the TYR gene—28 patients. Among the 20 patients with a single heterozygous variant in the TYR gene, 15 patients had the c.575C>A p.(Ser192Tyr) variant, and 15 had the c.1205G>A p.(Arg402Gln) variant. Both the c.575C>A p.(Ser192Tyr) and c.1205G>A p.(Arg402Gln) variants were identified in 12 patients. In addition to the aforementioned variants, an intronic variant c.1185-6208A>G (rs147546939) was identified in seven patients. Conclusions: the frequencies and the number of alleles c.575A, c.1205A, and c.1185-6208G in different groups of patients and the control group were compared. In this study, we demonstrate that the complex alleles [c.575C>A p.(Ser192Tyr); c.1205G>A p.(Arg402Gln)] and [c.575C>A p.(Ser192Tyr); c.1185-6208A>G; c.1205G>A p.(Arg402Gln)] are associated with oculocutaneous albinism, which is consistent with findings from other researchers.
Potent Biological Activity of Fluorinated Derivatives of 2-Deoxy-d-Glucose in a Glioblastoma Model
Authors: Sołtyka-Krajewska Maja , Ziemniak Marcin , Zawadzka-Kazimierczuk Anna , Skrzypczyk Paulina , Siwiak-Niedbalska Ewelina , Jaśkiewicz Anna , Zieliński Rafał , Fokt Izabela , Skóra Stanisław , Koźmiński Wiktor , Woźniak Krzysztof , Priebe Waldemar , Pająk-Tarnacka Beata
Published: October 2024

Background: One defining feature of various aggressive cancers, including glioblastoma multiforme (GBM), is glycolysis upregulation, making its inhibition a promising therapeutic approach. One promising compound is 2-deoxy-d-glucose (2-DG), a d-glucose analog with high clinical potential due to its ability to inhibit glycolysis. Upon uptake, 2-DG is phosphorylated by hexokinase to 2-DG-6-phosphate, which inhibits hexokinase and downstream glycolytic enzymes. Unfortunately, therapeutic use of 2-DG is limited by poor pharmacokinetics, suppressing its efficacy. Methods: To address these issues, we synthesized novel halogenated 2-DG analogs (2-FG, 2,2-diFG, 2-CG, and 2-BG) and evaluated their glycolytic inhibition in GBM cells. Our in vitro and computational studies suggest that these derivatives modulate hexokinase activity differently. Results: Fluorinated compounds show the most potent cytotoxic effects, indicated by the lowest IC50 values. These effects were more pronounced in hypoxic conditions. 19F NMR experiments and molecular docking confirmed that fluorinated derivatives bind hexokinase comparably to glucose. Enzymatic assays demonstrated that all halogenated derivatives are more effective HKII inhibitors than 2-DG, particularly through their 6-phosphates. By modifying the C-2 position with halogens, these compounds may overcome the poor pharmacokinetics of 2-DG. The modifications seem to enhance the stability and uptake of the compounds, making them effective at lower doses and over prolonged periods. Conclusions: This research has the potential to reshape the treatment landscape for GBM and possibly other cancers by offering a more targeted, effective, and metabolically focused therapeutic approach. The application of halogenated 2-DG analogs represents a promising advancement in cancer metabolism-targeted therapies, with the potential to overcome current treatment limitations.
The Effect of Drugs on the Intestinal Microbiota in Crohn’s Disease
Authors: Yang Xue , Zhang Yinghui , Gao Caiping , Pan Yan , Du Shan , Xiao Shiyu , Zhou Zhou
Published: October 2024

Objective: We took advantage of a single-center cross-sectional study to investigate the effect of different drugs on intestinal microbiota and function in Crohn’s disease. Methods: We studied the difference in fecal microbiota of Crohn’s disease patients treated with mesalazine, azathioprine, and infliximab, as well as untreated patients, by metagenome and screened for differential microbiota. Further, we investigated functional differences in intestinal microbiota among the four groups. Results: Through metagenomic sequencing, we found that there was no difference between the four groups in ACE and Chao1 indices, but IFX and mesalazine improved species diversity and homogeneity compared to the untreated group, as evidenced by statistically significant differences in the Shannon index, Simpson index, and pielou_evenness. In addition, beta diversity suggested a difference between groups, but the difference was not significant. Non-parametric tests revealed differences between the four groups at the phylum level, class level, and genus level. Further analysis by LEfSe analysis revealed that the level of short-chain fatty acid-producing microbiota was increased in the treated groups, while there was no difference between the treated groups when compared to each other. Finally, the KEGG database and EggNOG database revealed that there were functional differences in intestinal microbiota among the four groups, including microbial metabolism pathway, cysteine and methionine metabolism pathway, cytoskeleton, etc. Conclusions: Mesalazine, azathioprine, and infliximab can all affect the intestinal microbiota and function in patients with Crohn’s disease, and the drugs may alleviate intestinal inflammation in patients with Crohn’s disease by modulating the intestinal microbiota.
Advances in Research on Semi-Synthesis, Biotransformation and Biological Activities of Novel Derivatives from Maslinic Acid
Authors: Trabelsi Yosra , Znati Mansour , Ben Jannet Hichem , Bouajila Jalloul
Published: October 2024

Since ancient times, humans have turned to medicinal plants for treating various ailments and curing specific diseases, as these natural plants serve as the primary source of a range of phytochemicals, including triterpenes. Maslinic acid (MA), also known as (2α,3β)-2,3-dihydroxyolean-12-en-28-oic acid, is a pentacyclic triterpene acid present in numerous plants including olive, known for its high safety profile in humans. Recent experimental data increasingly suggests that MA exhibits diverse biological properties and therapeutic effects on various organ diseases, highlighting its significant potential for clinical applications due to its diverse potential pharmacological activities that promote health and resist various diseases, such as hypoglycemic, neuroprotective, anti-tumor, anti-inflammatory, antioxidant and multiple other biological activities. However, the undesirable pharmacokinetic properties of MA, such as high lipophilicity, pose a limitation to its application and development, impacting its bioavailability. Consequently, extensive research spanning decades has focused on structurally modifying MA to overcome these limitations and enhance its pharmacokinetic and therapeutic characteristics, leading to the identification of several potential lead compounds. In this review, we focus on the progress of research in recent years on MA with interest to its chemical and enzymatic modifications as well as the relationships between the modified structures or derivatives and their biological activities.
Antiproliferative Activity of an Organometallic Sn(IV) Coordination Compound Based on 1-Methylbenzotriazole against Human Cancer Cell Lines
Authors: Stamou Christina , Gourdoupi Chrisavgi , Dechambenoit Pierre , Papaioannou Dionissios , Piperigkou Zoi , Lada Zoi G.
Published: October 2024

A motivating class of compounds with interest in the research field of biological active metallopharmaceuticals for cancer treatment is based on organometallic complexes of Sn(IV), exhibiting advantages such as improved cellular uptake and body excretion, lower toxicity, and fewer side effects compared to platinum-based drugs. In this study, the mononuclear organotin coordination complex [(CH3)2SnCl2(mebta)2] was synthesized and characterized using vibrational spectroscopy (IR, Raman), 1H NMR, 13C{1H} NMR, and X-ray crystallography. Its antiproliferative properties were thoroughly assessed across an aggressive triple-negative human breast cancer cell line. Notably, comparative studies with precursor materials verified that the observed biological activity is intrinsic to the complex itself. This study highlights the compound’s ability to induce cell fate by disrupting essential cellular functions, such as proliferation. By exploring the antiproliferative effects of organotin(IV) derivatives, we introduce a novel class of Sn complexes with 1-methylbenzotriazole (mebta), demonstrating significant potential as promising antitumor agents in the field of organotin compounds.
Assessment of Optical and Scanning Electron Microscopies for the Identification and Quantification of Asbestos Fibers and Typical Asbestos Bodies in Human Colorectal Cancer Tissues
Authors: Croce Alessandro , Bertolotti Marinella , Bellis Donata , Glorioso Alex , Bertolina Carlotta , Farotto Marianna , Giacchero Fabio , Roveta Annalisa , Maconi Antonio
Published: October 2024

Asbestos research, identification, and quantification have been performed over the years, and the relationship between fiber inhalation and lung disease development is well defined. The same cannot be said for the gastroenteric system: the International Agency for Research on Cancer (IARC) believes that colorectal cancer (CRC) could be associated with asbestos exposure, but research has not demonstrated a casual nexus between exposure and CRC, despite highlighting an association tendency. The combination of scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS) is the most applied technique in asbestos fiber identification in tissues and intestinal mucosa. In this study, SEM/EDS was applied to evaluate the presence of asbestos fibers and bodies (ABs) inside the tissue of eleven patients affected by CRC who had undergone environmental exposure due to living in an asbestos-polluted area where an Eternit plant had been active in the past. This technique was coupled with optical microscopy (OM) to verify whether the latter could be applied to evaluate the presence of these mineral phases, with the goal of understanding its suitability for identifying fibers and ABs in colon tissues. In addition to verifying the presence of fibers, this study allowed us to identify the deposition site of said fibers within the sample and possibly detect associated tissue reactions using OM, over a shorter time and at lower costs. Despite being a preliminary and descriptive work, the obtained results allowed us to propose a method involving first-sample OM observation to identify regulated (fibers with a length ≥ 5 μm, a thickness ≤ 3 μm, and a length/thickness ratio > 3) asbestos phases and ABs in the extra-respiratory system. In fact, OM and SEM/EDS provided similar information: no asbestiform morphology or ABs were found, but phyllosilicates and other inorganic materials were identified. This research needs to be continued using higher-resolution techniques to definitively rule out the presence of these fibers inside tissues whilst also increasing the number of patients involved.
Comment on Alfageme-Garcia et al. Respectful Children’s Shoes: A Systematic Review. Children 2024, 11, 761
Authors: Gijon-Nogueron Gabriel
Published: October 2024

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