bluuebunny/crossref_metadata_2025 huggingface dataset:
Factors associated with fluoxetine and norfluoxetine plasma concentrations and clinical response in Mexican patients with mental disorders
Authors: Sagahón‐Azúa Julia , Medellín‐Garibay Susanna Edith , Chávez‐Castillo Cinthya Eloisa , González‐Salinas César Guillermo , Milán‐Segovia Rosa del Carmen , Romano‐Moreno Silvia
Published: September 2021

Over the last few years, fluoxetine has been one of the most prescribed medications for the treatment of diverse psychiatric conditions in Mexico. Fluoxetine therapeutic effect is consequence of the joint action of the parent drug and its active metabolite, norfluoxetine. However, the clinical efficacy of fluoxetine, can be affected due to diverse factors, such as drug‐drug interactions and the large interindividual variability in the pharmacokinetics of this drug. The aim of this study was to determine the factors associated with variability in plasma concentrations of fluoxetine and norfluoxetine and its association with the therapeutic response. Fluoxetine and norfluoxetine plasma concentrations were quantified by liquid chromatography in 81 Mexican patients with mental disorders; 25% of the patients had no medication adherence and 40% were below the reference range of fluoxetine plus norfluoxetine plasma concentrations. The results showed that concentrations can be affected by fluoxetine metabolism caused by CYP2D6 phenotype and the concomitant administration of olanzapine. Furthermore, CYP3A5 and CYP2C19 phenotype were associated with lower anxiety and depression control during treatment with fluoxetine. This study can be a starting point to elucidate the causes of fluoxetine variable response in Mexican patients with mental disorders, as well as to detect and support medication adherence.
Distributed urban drag parametrization for sub‐kilometre scale numerical weather prediction
Authors: Sützl Birgit S. , Rooney Gabriel G. , Finnenkoetter Anke , Bohnenstengel Sylvia I. , Grimmond Sue , van Reeuwijk Maarten
Published: September 2021

A recently developed, height‐distributed urban drag parametrization is tested with the London Model, a sub‐kilometre resolution version of the Met Office Unified Model over Greater London. The distributed‐drag parametrization requires vertical morphology profiles in the form of height‐distributed frontal‐area functions, which capture the full extent and variability of building heights. London's morphology profiles are calculated and parametrized by an exponential distribution with the ratio of maximum to mean building height as the parameter. A case study evaluates the differences between the new distributed‐drag scheme and the current London Model setup using the MORUSES urban land‐surface model. The new drag parametrization shows increased horizontal spatial variability in total surface stress, identifying densely built‐up areas, high‐rise building clusters, parks, and the river. Effects on the wind speed in the lower levels include a lesser gradient and more heterogeneous wind profiles, extended wakes downwind of the city centre, and vertically growing perturbations that suggest the formation of internal boundary layers. The surface sensible heat fluxes are underpredicted, which is attributed to difficulties coupling the distributed momentum exchange with the surface‐based heat exchange.
Robust adaptive fault‐tolerant proportional‐derivative tracking control for six‐degrees of freedom unmanned aerial vehicles
Authors: Gao Zhen , Chen Yudong , Li Wenqiao , Song Yongduan
Published: September 2021

This article presents a robust adaptive control scheme for six‐degrees of freedom (DOF) unmanned aerial vehicles (UAVs) in the presence of modeling uncertainties and actuation faults. The proposed control is in proportional‐derivative (PD) form, and is able to tolerate actuation faults yet ensure stability and transient performance without the need for the detail model information of UAV. Furthermore, the PD control scheme exploited adaptively self‐tuning PD gains, which avoids the ad‐hoc and time‐consuming trial and error process for gain determination as commonly required in traditional PD control, thus is design‐friendly and low‐cost, rendering the control algorithms easy and straightforward for programming and implementation. Both theoretical analysis and numerical simulation validate the effectiveness of the proposed control method.
Hearing, voicing and healing: Rivers as culturally located and connected
Authors:   Martuwarra RiverOfLife,   Unamen Shipu Romaine River, Poelina Anne , Wooltorton Sandra , Guimond Laurie , Sioui Durand Guy
Published: September 2021

In this article, a collaborative writing group explores how we, two rivers, express ourselves over time, place and space, our energies long interpreted as veins and arteries carrying the Country's life affirming blood. Voiced as River: I, River, this position reflects a worldview in which interrelationship with living river is normal, and River Spirit is ever‐present. It is a position underpinned by Indigenous narratives as riverine expressions of place‐based love.

At times, the article is also voiced as a writing group or individuals, with voices being interchanged where required for smooth reading. We see this as part of the decolonising process, which feels liberating and healing among the writers. Each writer is equally valued as co‐creator, contributor, narrator and story teller.

The two rivers, being Martuwarra Fitzroy River (Kimberley, Western Australia) and Unamen Shipu Romaine River (North Shore, Québec, Canada), illustrate a common condition of being, through heritage, life, change and possibility. Through stories and voices, the socio‐scientific implications of colonisation and lost connections become clear, considering the interaction, the dialogue and the cultural synthesis of living water systems that have always incorporated all life forms into rivers of life. As a way of navigating towards wholeness, Aboriginal knowledge systems and narratives for healing are used to bring together findings of this intercultural river learning journey.
3D Printing and Pyrolysis of Optical ZrO2 Nanostructures by Two‐Photon Lithography: Reduced Shrinkage and Crystallization Mediated by Nanoparticles Seeds
Authors: Desponds Anne , Banyasz Akos , Chateau Denis , Tellal Azeddine , Venier Amandine , Meille Sylvain , Montagnac Gilles , Chevalier Jérôme , Andraud Chantal , Baldeck Patrice L. , Parola Stephane
Published: September 2021

Two‐photon lithography is a potential route to produce high‐resolution 3D ceramics. However, the large shrinkage due to the elimination of an important organic counterpart of the printed material during debinding/sintering remains a lock to further development of this technology. To limit this phenomenon, an original approach based on a composite resin incorporating 45 wt% ultrasmall (5 nm) zirconia stabilized nanoparticles into the zirconium acrylate precursor is proposed to process 3D zirconia microlattices and nanostructured optical surfaces. Interestingly, the nanoparticles are used both as seeds allowing control of the crystallographic phase formed during the calcination process and as structural stabilizing agent preventing important shrinkage of the printed ceramic. After 3D photolithography and pyrolysis, the weight and volume loss of the microstructures are drastically reduced as compared to similar systems processed with the reference resin without nanoparticles, and stable 3D microstructures of cubic zirconia are obtained with high spatial resolution. In the case of a patterned surface, the refractive index of 2.1 leads to a diffraction efficiency large enough to obtain microfocusing with linewidths of 0.1 µm, and the demonstration of a microlens array with a period as small as 0.8 µm.
Characterization of bending performance of reinforced cementitious composites beams with hybrid fibers after exposure to high temperatures
Authors: Li Li , Guan Junfeng , Xie Yan , Cao Mingli
Published: September 2021

The bending performance of reinforced beam containing steel fiber, polyvinyl alcohol (PVA) fiber, and calcium carbonate whisker after high temperature was studied. With the introduction of steel–PVA–whisker hybrid fibers, the thermal bursting and cracking of the reinforced beams were alleviated significantly. The peak load of reinforced beam was significantly increased with the introduction of 1.5 vol% steel fiber and 0.5 vol% PVA fiber. While the introduction of whisker led to decreased peak load and shear failure pattern of the beams, due to the decomposition of whisker at high temperature. The microscopic mechanism of action of fiber and whisker is discussed. A prediction model for the shear bearing capacity of reinforced beams with hybrid fibers after high temperature was proposed, considering the action of multiscale fibers and high temperature.
Evaluation of in vitro human skin models for studying effects of external stressors and stimuli and developing treatment modalities
Authors: Sutterby Emily , Thurgood Peter , Baratchi Sara , Khoshmanesh Khashayar , Pirogova Elena
Published: September 2021

Skin is exposed to a variety of potential stressors and stimulators that may impact homeostasis, healing, tumor development, inflammation, and irritation. As such it is important to understand the impact that these stimuli have on skin health and function, and to develop therapeutic interventions. Animal experiments have been the gold standard for testing the safety and efficacy of therapeutics and observing disease pathology for centuries. However, complex ethics, costs, time consumption, and interspecies variation limit the transferability of results to humans and reduce their repeatability and reliability. Furthermore, traditional 2D cell studies are not representative of human tissue. Skin tissue is a dynamic environment, and when cells are isolated in unphysiologically stiff, static petri dishes their behavior, and phenotypic expression is altered. Increasingly complex in vitro models of human skin, including organoids, 3D bioprinting, and skin‐on‐a‐chip platforms, present the opportunity to gain insight into how stressors affect tissue at a cellular level in a controlled and repeatable environment. This insight can be leveraged to further understand pathological skin conditions and better formulate and validate drugs and therapeutics. Here, we will discuss the application of in vitro skin modeling to investigating the effects of mechanical, electromagnetic, and chemical stressors on skin.
Prophylactic effects of dietary ascorbic acid on oxidative stress indices, physiological and behavioural responses of domestic pigeons exposed to road transport stress
Authors: Hosseinian S.A. , Ansari S.
Published: September 2021

Transport disturbs birds’ welfare and health status which lead to oxidative stress and dietary ascorbic acid mitigates the adverse effects of transport stress. The present study was aimed to evaluate the impacts of ascorbic acid administration on oxidative stress indices, cortisol, H/L ratio, tonic immobility reaction and rectal temperature of pigeons exposed to road transport. A total of 80 clinically healthy pigeons were selected and randomly divided to eight equal groups as follow: (1) Ctrl–: fed by basal diet and no subjected to transport stress; (2) Ctrl+: fed by the basal diet and subjected to transport stress; (3, 4) 1DBS10 and 1DBS16: received ascorbic acid from 1 day before transport stress at doses of 10 g/100 L and 16 g/100 L of drinking water, respectively; (5, 6) 3DBS10 and 3DBS16: treated with ascorbic acid from 3 consecutive days before transport stress at doses of 10 g/100 L and 16 g/100 L, respectively and (7, 8) 7DBS10 and 7DBS16: received ascorbic acid from 7 consecutive days before the transport at doses 10 g/100 L and 16 g/100 L, respectively. Birds were transported for 3 h over a distance of about 200 km. The total antioxidant capacity, malondialdehyde and cortisol were measured before transport and at 6, 24 and 72 h post‐transportation. The rectal temperature and tunic immobility reactions were recorded. Dietary ascorbic acid led to a decrease in tonic immobility response, hetrophil to lymphocyte ratio, circulating cortisol and total antioxidant capacity, and an increase in circulating malondialdehyde in pigeons exposed to transport stress compare to Ctrl+ group. In conclusion, ascorbic acid administration at dose 16 g/100 L of drinking water from 3 and 7 days before exposure to stress helps attenuate undesirable effects of oxidative stress in pigeons.
Medizinische Zwillingsforschung in Deutschland
Authors: Enck Paul , Goebel-Stengel Miriam , Rieß Olaf , Hübener-Schmid Jeannette , Kagan Karl Oliver , Nieß Andreas Michael , Tümmers Henning , Wiesing Urban , Zipfel Stephan , Stengel Andreas ,   TwinHealth-Konsortium, Dufke Andreas , Brucker Sara Y. , Linneweh Miriam , Fischer Katja , Renner Tobias , Frick Julia-Stefanie , Martus Peter , Nahnsen Sven , Weimer Katja
Published: September 2021

Nach dem Zweiten Weltkrieg wurden weltweit Zwillingskohorten aufgebaut, die inzwischen ca. 1,5 Mio. Zwillinge umfassen und zwischen 1950 und 2012 über 2748 Zwillingsstudien hervorgebracht haben. Diese Zahl steigt jedes Jahr um weitere 500 bis 1000. Die Unterrepräsentanz deutscher Zwillingsstudien in diesen Datenbanken lässt sich nicht allein durch den Missbrauch medizinischer Forschung im Nationalsozialismus erklären. Entwicklung und Ausbau großer Zwillingskohorten sind ethisch und datenschutzrechtlich eine Herausforderung. Zwillingskohorten ermöglichen jedoch die Langzeit- und Echtzeiterforschung vieler medizinischer Fragestellungen; und die Zwillingsstudien tragen auch nach der Entschlüsselung des Humangenoms erheblich zur Beantwortung der Frage nach Anlage oder Umwelt als mögliche Erkrankungsauslöser bei.

Derzeit gibt es 2 deutsche Zwillingskohorten: die biomedizinische KohorteHealthTwiStmit ca. 1500 Zwillingspaaren undTwinLife, eine soziologisch-psychologische Kohorte mit ca. 4000 Zwillingspaaren. Daneben gibt es krankheitsspezifische Kohorten. 2016 startete dasTwinHealth-Konsortium der Medizinischen Fakultät der Universität Tübingen mit dem Ziel, eine forschungsoffene und nachhaltige Zwillingsforschung am Standort Tübingen zur Bearbeitung unterschiedlicher Fragestellungen zu etablieren.

Der Artikel bietet mithilfe einer systematischen Literaturrecherche und einer medizinhistorischen Betrachtung einen Überblick über die weltweite und nationale Entwicklung von Zwillingsstudien und -datenbanken der letzten 100 Jahre. Anhand der TübingerTwinHealth-Initiative beleuchtet er den Aufbau eines Zwillingskollektivs und dessen juristische, ethische und Datenschutzaspekte.
Experimental and numerical investigations on heat transfer in fused filament fabrication 3D-printed specimens
Authors: Ramos Nathalie , Mittermeier Christoph , Kiendl Josef
Published: September 2021

A good understanding of the heat transfer in fused filament fabrication is crucial for an accurate stress prediction and subsequently for repetitive, high-quality printing. This work focuses on two challenges that have been presented when it comes to the accuracy and efficiency in simulating the heat transfer in the fused filament fabrication process. With the prospect of choosing correct thermal boundary conditions expressing the natural convection between printed material and its environment, values for the convective heat transfer coefficient and ambient temperature were calibrated through numerical data fitting of experimental thermal measurements. Furthermore, modeling simplifications were proposed for an efficient numerical discretization of infill structures. Samples were printed with varying infill characteristics, such as varying air void size, infill densities and infill patterns. Thermal measurements were performed to investigate the role of these parameters on the heat transfer and based on these observations, possible modeling simplifications were studied in the numerical simulations.
Auftragsmord und Anstiftung zum Mord als Varianten des Intimizids
Authors: Dietz E. , Schädler J. , Püschel K. , Ondruschka B.
Published: September 2021

Dargestellt wird der Fall eines Intimizids, bei dem sich ein Ehemann durch Anstiftung zum Mord seiner Ehefrau entledigen wollte. Auftragsmorde bzw. Anstiftungen zum Mord innerhalb von Paarbeziehungen stellen ein Ausnahmegeschehen dar. Hier sollen die Facetten einer umfangreichen kriminalistischen Fallbearbeitung dargestellt werden, die neben rechtsmedizinischen Erkenntnissen auch wesentliche psychiatrische, psychologische und kriminalistische Aspekte der Tatbegehung bzw. -anstiftung beleuchtet.
„Skills fOr Life Adjustment and Resilience“ Programm
Authors: Lotzin Annett , Hinrichsen Imke , Kenntemich Laura , Freyberg Renée-Christin , Lau Winnie , Gibson Kari , O’Donnell Meaghan
Published: September 2021

Hintergrund

Nach Katastrophen, Traumata und anderen schweren Belastungen entwickelt ein Teil der Betroffenen eine psychische Erkrankung, während ein weiterer Teil anhaltende subklinische Belastungen zeigt, die das psychosoziale Funktionsniveau einschränken. Anhaltend subklinisch belastete Menschen erhalten jedoch selten psychosoziale Unterstützungsangebote.

Ziel der Arbeit

Vorgestellt wird das Programm „Skills fOr Life Adjustment and Resilience“ (SOLAR), eine Kurzintervention für Menschen mit anhaltender subklinischer Belastung nach Katastrophen und anderen schweren Belastungen. Erste Ergebnisse einer Pilotstudie zur Zufriedenheit mit dem Programm bei Betroffenen werden berichtet.

Methoden

Das SOLAR-Programm wurde von einem internationalen Konsortium, zusammengesetzt aus Katastrophen- und Traumaexperten, entwickelt. Es beinhaltet 5 wöchentliche Gruppensitzungen, in denen verhaltenstherapeutische Elemente vermittelt werden. Im Rahmen der Pilotstudie nahmen 15 Teilnehmende im Präsenzformat und 15 Teilnehmende mithilfe einer Videokonferenzschaltung während der „Coronavirus Disease 2019“ (COVID-19) Pandemie am SOLAR Programm teil. Nach Abschluss beantworteten die Teilnehmenden den Fragebogen zur Messung der Patientenzufriedenheit (ZUF-8).

Ergebnisse

Die Teilnehmenden waren „weitgehend“ bis „sehr zufrieden“. In der Präsenzgruppe war die Zufriedenheit über alle Aspekte hinweg geringfügig stärker ausgeprägt als in der Onlinegruppe. Die TrainerInnen bewerteten das Programm als gut durchführbar.

Schlussfolgerung

Das SOLAR-Programm ist eine vielversprechende Kurzintervention bei anhaltender subklinischer Belastung nach schweren Belastungen, die im Präsenz- oder im videogestützten Format weiter auf seine Wirksamkeit erprobt werden sollte. Im Beitrag werden Praxisempfehlungen zur Durchführung gegeben.
CFD analysis on the oil flow of a gear stage with guide plate
Authors: Hildebrand Lucas , Dangl Florian , Sedlmair Martin , Lohner Thomas , Stahl Karsten
Published: September 2021

Gearbox housing geometry and oil guide plates strongly influence gearbox oil flow and interaction of oil with machine elements. Guided oil flow can increase gearbox efficiency and improve heat management. Recent research studies have demonstrated the potential of Computational Fluid Dynamics (CFD) simulations to predict the gearbox oil flow and no-load losses. Thereby, the influence of housing geometry and guide plates has rarely been addressed. This study focuses on a CFD analysis on the oil flow of a dip lubricated spur gear stage with a guide plate. Grid-based CFD models with different simulation setups were confronted and evaluated. Results show that the selection of the simulation setup with respect to the acceleration ramp and mesh size needs to address the considered object of investigation and the desired depth of information. An appropriate simulation setup shows great accordance with recordings of the oil distribution by a high-speed camera. A detailed analysis of the simulation results identified the contribution of different gear surface zones to the no-load gear loss torque. For the considered guide plate a strong interaction of oil flow and loss torque due to pressure forces on the tooth flank surface zones and due to shear forces on the front and tip circle surface zones of the gears was determined.
Approach for multiscale modeling the thermomechanical tool load in gear hobbing
Authors: Troß Nico , Brimmers Jens , Bergs Thomas
Published: September 2021

In this report, an approach is presented how a geometric penetration calculation can be combined with FE simulations to a multiscale model, which allows an efficient determination of the thermomechanical load in gear hobbing. FE simulations of the linear-orthogonal cut are used to derive approximate equations for calculating the cutting force and the rake face temperature. The hobbing process is then simulated with a geometric penetration calculation and uncut chip geometries are determined for each generating position. The uncut chip geometries serve as input variables for the derived equations, which are solved at each point of the cutting edge for each generating position. The cutting force is scaled according to the established procedure of discrete addition of the forces along the cutting edge over all individual cross-section elements. For the calculation of the temperature, an approach is presented how to consider a variable chip thickness profile. Based on this, the temperature distribution on the rake face is calculated. The model is verified on the one hand by cutting force measurements in machining trials and on the other hand by an FE simulation of a full engagement of a hob tooth.
Comparison results for solutions to p-Laplace equations with Robin boundary conditions
Authors: Amato Vincenzo , Gentile Andrea , Masiello Alba Lia
Published: September 2021

In the last decades, comparison results of Talenti type for Elliptic Problems with Dirichlet boundary conditions have been widely investigated. In this paper, we generalize the results obtained in Alvino et al. (Commun Pure Appl Math, to appear) to the case of p-Laplace operator with Robin boundary conditions. The point-wise comparison, obtained in Alvino et al. (to appear) only in the planar case, holds true in any dimension if p is sufficiently small.
Suicidality and Personality Pathology in Adolescence: A Systematic Review
Authors: Moselli Marta , Casini Maria Pia , Frattini Camilla , Williams Riccardo
Published: September 2021

This work presents a review of research papers examining the role of emerging personality pathology in suicidal ideation and behaviours in adolescence. Initially, 226 studies were selected in line with PRISMA guidelines, and 33 articles were finally included in this review. The data show percentages of any personality disorder diagnosis ranging from 19.5 to 22.8% in suicide attempters, while in autopsy studies, the rate of personality disorder diagnosis varied between 29.6 and 42.1%. The overwhelming majority of the studies focus on the role of borderline personality disorder (BPD) in suicidal behaviours, also highlighting its predictive role at a longitudinal level. Furthermore, the literature review shows that personality traits supposed to underlie BPD, such as affective instability, impulsivity and identity diffusion, have specific predictive links with suicidal conduct. Other personality pathology dimensions, such as aggressiveness, sadism and perfectionism that are associated with other personality disorders, namely, antisocial and narcissistic personality disorders, have also shown a significant mediating role for suicidal risk. Overall, these results seem to parallel the role of personality pathology in predicting suicide in adulthood and point to the relevance of assessing the presence of emerging patterns of personality disorders for the clinical management of suicidal risk in adolescence.
Self-adaptive macroslip array for friction force prediction in contact interfaces with non-conforming meshes
Authors: Battiato Giuseppe
Published: September 2021

The steady-state nonlinear forced response (NFR) of finite element (FE) models with friction joints is usually computed in the frequency domain through the combination of node-to-node contact elements and the Harmonic Balance Method (HBM). In the current state of the art, rare are the cases where the friction forces are estimated for contact interfaces with non-conforming mesh grids. This need is nowadays taking place due to the improving capability of commercial FE software to manage any kind of boundary condition (i.e., either coupling or contact), without requiring coincident pairs of nodes at the joint interfaces. Such an advantage becomes a drawback when the analysts are requested to investigate the NFR of the assembly by using build-in codes, where the contact forces prediction usually requires node-to-node contact elements whose parameters (i.e., the contact stiffnesses and friction coefficients) can be easily identified by means of experiments. This paper addresses the mentioned limitation, and proposes a novelself-adaptive macroslip array(SAMA) model for the estimation of the nonlinear friction forces on FE contact interfaces with non-conforming meshes. The SAMA model consists on a set of node-to-node contact elements ordered in parallel, whose contact parameters and normal preloads are identified through a step-by-stepself-adaptiveweighting algorithm that depends on the topology of the meshes in contact. The goodness of the proposed model is assessed on the calculation of the NFR of a bladed disk with shroud contacts, under the hypotheses of cyclic symmetry and HBM. The nonlinear dynamic behavior of the bladed disk is evaluated in two different cases. First, in the case of lack of node-to-node congruence at the contact interface for the structure being in its undeformed configuration, and second, in the case of a relevant static misalignment of the contact interfaces due to the application of large static loads.
The Effect of Additive Chemical Structure on the Tribofilms Derived from Varying Molybdenum-Sulfur Chemistries
Authors: Barnes Aaron L. , Morina Ardian , Andrew Rhiann E. , Neville Anne
Published: September 2021

Molybdenum disulfide (MoS2) is an effective friction modifier that can be formed on surfaces from oil-soluble lubricant additives. Different additive chemistries can be used to form MoS2 on a surface. The tribofilms formed from three different molybdenum additives (MoDTC Dimer, MoDTC Trimer, and molybdate ester) were studied in additive monoblends and fully formulated systems. The resulting tribofilms were then characterized by Raman spectroscopic spatial mapping, XPS, and FIB-TEM. The distribution of MoS2 on the surface was much more sparse for the molybdate ester than the other additives. No crystalline molybdenum oxides were observed by Raman spectroscopy, but their presence was inferred from XPS analysis. XPS analysis showed very similar distributions of Mo oxidation states from each additive, such that the chemical nature of the films formed from all of the additives is likely similar. Each of the additive tribofilms was observed to have MoS3 vibrations in Raman and persulfide XPS peaks associated with amorphous MoS3, as such this species is presented as a common frictional decomposition product for all the additives. The MoDTC trimer is more able to produce this amorphous species on the contacting surfaces due to its structural similarities to the co-ordination polymer MoS3.

Graphical Abstract
MiR-200c-3p aggravates gastric cell carcinoma via KLF6
Authors: Wang Ying , Lu Kaijuan , Li Weibing , Wang Zhigang , Ding Jing , Zhu Zeyu , Li Zhipeng
Published: September 2021

Background

Gastric cell carcinoma (GCC) is a common and high-incidence malignant gastrointestinal cancer that seriously threatens human life and safety. Evidences suggest that microRNAs (miRNAs) exhibit an essential role in regulating the occurrence and development of GCC, while the effects and possible mechanisms remain to be further explored.

Objective

This study was designed to explore whether miR-200c-3p exerted its functional role in the growth and metastasis of GCC, and investigate the possible mechanisms.

Methods

The expression levels of miR-200c-3p in GCC tissues and cell lines were detected by qRT-PCR analysis. The functional role of miR-200c-3p in the viability, proliferation, migration and invasion of GCC cells were evaluated by CCK-8, EdU, wound healing and Transwell assays. In addition, the candidate targets of miR-200c-3p was predicted and confirmed by dual-luciferase reporter assay. Moreover, the relationship between miR-200c-3p and target (Krüppel like factor 6, KLF6) was assessed by qRT-PCR and western blot assays. Besides, the expression levels of KLF6 in GCC cells were determined by qRT-PCR and western blot assays. Furthermore, the role of KLF6 in the viability, proliferation, migration and invasion of GCC cells mediated with miR-200c-3p mimics was evaluated by CCK-8, EdU, wound healing and Transwell assays.

Results

In the present study, a new tumor promoting function of miR-200c-3p was disclosed in GCC. We found that the expression of miR-200c-3p was obviously increased in clinic GCC tissues and cell lines. In addition, down-regulation of miR-200c-3p suppressed cell viability, proliferation, migration, and invasion in GCC cells. Moreover, KLF6 was verified as a direct target of miR-200c-3p by binding its 3’-UTR. Additionally, KLF6 was remarkably decreased and was negatively associated with the miR-200c-3p expression in GCC cell lines. Furthermore, over-expression of KLF6 retarded the effects of miR-200c-3p on the growth and metastasis of GCC cell lines.

Conclusions

MiR-200c-3p potentially played a tumor-promoting role in the occurrence and development of GCC, which may be achieved by targeting KLF6.

Graphic abstract
DPM model segregation validation and scaling effect in a rotary drum
Authors: Patil Amit V. , Hofsteenge Jesse , Bujalski Jakub M. , Johansen Stein T.
Published: September 2021

Discrete phase method (DPM) model was used to analyse rotary drum systems for segregation behavior. DPM simulations were performed for comparison with a dynamic segregation experimental measurement from the literature. This included dynamic segregation and time-averaged particle velocity field, which were validated with experimental data. In addition, a direct DPM and parcel scaled DPM simulation study was performed to analyse the effect of drum and particle parcel size scaling. The segregation dynamics was evaluated using the Lacey mixing index. This work shows segregation dynamics decreases with increasing drum size while keeping the same particle size. It further shows that for a given drum size the segregation dynamics deviate after a certain particle parcel scaling limit. The parcel scaling limit also increases with increasing drum size.
Characteristic strength and acoustic emission properties of weakly cemented sandstone at different depths under uniaxial compression
Authors: Liu Bin , Zhao Yixin , Zhang Cun , Zhou Jinlong , Li Yutao , Sun Zhuang
Published: September 2021

As coal mining is extended from shallow to deep areas along the western coalfield, it is of great significance to study weakly cemented sandstone at different depths for underground mining engineering. Sandstones from depths of 101.5, 203.2, 317.3, 406.9, 509.9 and 589.8 m at the Buertai Coal Mine were collected. The characteristic strength, acoustic emission (AE), and energy evolution of sandstone during uniaxial compression tests were analyzed. The results show that the intermediate frequency (125–275 kHz) of shallow rock mainly occurs in the postpeak stage, while deep rock occurs in the prepeak stage. The initiation strength and damage strength of the sandstone at different depths range from 0.23 to 0.50 and 0.63 to 0.84 of peak strength (σc), respectively, decrease exponentially and are a power function with depth. The precursor strength ranges from 0.88σc to 0.99σc, increases with depth before reaching a depth of 300 m, and tends to stabilize after 300 m. The ratio of the initiation strength to the damage strength (k) ranges from 0.25 to 0.62 and decreases exponentially with depth. The failure modes of sandstone at different depths are tension-dominated mixed tensile-shear failure. Shear failure mainly occurs at the unstable crack propagation stage. The count of the shear failure bands before the peak strength increases gradually, and increases first and then decreases after the peak strength with burial depth. The cumulative input energy, released elastic energy and dissipated energy increase with depth. The elastic release rate ranges from 0.46 × 10–3 to 198.57 × 10–3 J/(cm3 s) and increases exponentially with depth.
‘Come on, man!’ On errors, choice, and Hayekian behavioral economics
Authors: Sunstein Cass R.
Published: September 2021

With respect to the views of dead thinkers, answers to many particular questions are often interpretive in Ronald Dworkin's sense. Such answers must attempt (1) to fit the materials to be interpreted and (2) to justify them, that is, to put them in the best constructive light. What looks like (1), or what purports to be (1), is often (2). That is, when a follower of Kant urges that ‘Kant would say x’, or that ‘Kantianism entails y’, the goal is to make the best constructive sense of Kant and Kantianism, not merely to adhere to something that Kant actually said. An approach to behavioral economics cannot claim to be Hayekian if it is rooted in enthusiasm for the abilities of planners to set prices and quantities, or if it sees the price system as a jumble of mistakes and errors. But within a not-so-narrow range, a variety of freedom-preserving approaches, alert to the epistemic limits of planners, can fairly claim to be Hayekian. Hayekian behavioral economics, I suggest, is an approach that (1) recognizes the importance and pervasiveness of individual errors, (2) emphasizes the epistemic limits of planners, (3) builds on individual choices rather than planner preferences, and (4) gives authority to choices made under epistemically favorable conditions, in which informational deficits and behavioral biases are least likely to be at work. The key step, of course, is (4). If it is properly elaborated, the resulting approach deserves respect. It is worthy of serious consideration, even if some of us, including the present author, would not entirely embrace it. In defending that proposition, the present essay responds to some critical remarks on behaviorally informed policy, including the resort to ‘explainawaytions’ (Matthew Rabin's term) for behavioral findings.
The Protection of Human Rights in Pandemics—Reflections on the Past, Present, and Future
Authors: Dąbrowska-Kłosińska Patrycja
Published: September 2021

This special section tells the story of Covid-19 through the lens of national responses, serious concerns about unprecedented human rights limitations and infringements, and the respective role of courts in public health emergencies. It compiles perspectives on disease control developments in Brazil, Italy, Poland, Taiwan, the U.S., and the EU to explore various aspects of judicial review protecting, or failing to protect, human rights. It offers insights from states and regions which have experienced high pandemic rates or may attract attention for not treating human rights as a priority. Amidst the crisis of multilateralism and the World Health Organization (WHO) authority, and the fact that public health is typically a national power, the Articles focus on the state-level analyses to inspire comparative findings and further research. The section also draws on diversity and transdisciplinarity. The contributions are authored by scholars specializing in wide-ranging areas of law, including constitutional, health, private, and human rights law, as well as in political philosophy and public health. This text introduces the special section by offering a broader picture of the human rights’ problématique in times of pandemics.
Prevalence and underlying factors of mobile game addiction among university students in Bangladesh
Authors: Sayeed Md Abu , Rasel Md Shabbir Rahman , Habibullah Abrar Ahamed , Hossain Md Moyazzem
Published: September 2021

Background

Nowadays, the youth are more engaging with their more advanced smartphones having high-quality graphics and gaming features. However, existing literature depicts that adolescents suffer from several forms of psychological problems including mental health, depression, loneliness, insomnia and low self-control due to mobile game addiction. Therefore, this study aims to find the prevalence and motivating factors for mobile game addiction among university students of Bangladesh.

Methods

A cross-sectional survey was carried out to collect the required information from 1125 students of three universities in Bangladesh. Descriptive statistics, χ2 test and ordinal regression model are employed to meet the objective of this study.

Results

The findings reveal that male students are more likely to show addictive behaviours than their counterparts in the context of mobile game addiction. The results depict that loneliness, duration of using smartphones and playing mobile games, and source of entertainment are the main cause of mobile addiction. Also, more than half of the respondents (54.3%) are severely addicted to mobile games who were influenced by friends and YouTube gamers to play games. Moreover, students are suffering from several physical problems such as headaches, eye discomfort, blurry vision and ear discomfort.

Conclusion

Considering the findings of this paper, the authors suggest that the authorities should consider this immediately and arrange a positive entertainment environment to prevent students from mobile games. Furthermore, it is necessary to encourage students to participate in sports or other extracurricular activities that may be helpful to lessen mobile game addiction among students in Bangladesh.
How Democracy Works within a Populist Party: Candidate Selection in the Alternative for Germany
Authors: Höhne Benjamin
Published: September 2021

Does the demand for more direct democracy by populist parties have any implications for their internal decision-making? To answer this question, a novel large-scale research project analyses the 2017 candidate selection of all Bundestag parties, including the populist Alternative for Germany. Some 1,334 individual nominations of seven parties are compared using quantitative indicators along three dimensions of intra-party democracy (IPD): competition between aspirants for candidacy, inclusion of members and nomination-related communication. It shows that the AfD is living up to its promise of practising grassroots democracy: in all results it ranks at the top by a wide margin. A new populist organizational model seems to have emerged following neither the classic hierarchical and leader-oriented mode of many other European right-wing populist parties nor the delegate assembly mode typical of German parties. Our further development of IPD concepts, newly elaborated measuring methods and surprising empirical evidence improve the understanding of democratic decision-making in populist parties.
Does Conjoint Analysis Mitigate Social Desirability Bias?
Authors: Horiuchi Yusaku , Markovich Zachary , Yamamoto Teppei
Published: September 2021

How can we elicit honest responses in surveys? Conjoint analysis has become a popular tool to address social desirability bias (SDB), or systematic survey misreporting on sensitive topics. However, there has been no direct evidence showing its suitability for this purpose. We propose a novel experimental design to identify conjoint analysis’s ability to mitigate SDB. Specifically, we compare a standard, fully randomized conjoint design against a partially randomized design where only the sensitive attribute is varied between the two profiles in each task. We also include a control condition to remove confounding due to the increased attention to the varying attribute under the partially randomized design. We implement this empirical strategy in two studies on attitudes about environmental conservation and preferences about congressional candidates. In both studies, our estimates indicate that the fully randomized conjoint design could reduce SDB for the average marginal component effect (AMCE) of the sensitive attribute by about two-thirds of the AMCE itself. Although encouraging, we caution that our results are exploratory and exhibit some sensitivity to alternative model specifications, suggesting the need for additional confirmatory evidence based on the proposed design.
Sinuous stromatolites of the Chandi Formation, Chattisgarh Basin, India: their origin and implications for Mesoproterozoic seawater
Authors: Słowakiewicz Mirosław , Banerjee Amlan , Patranabis-Deb Sarbani , Kumar Deb Gautam , Tucker Maurice E.
Published: September 2021

Remnants of some of the planet’s most ancient life forms, stromatolites in the late Mesoproterozoic sea of the Chattisgarh Basin, India, preserve a conspicuous sinuous pattern. They occur as successive biostromes, 10–30 cm thick, separated by 2–5-cm-thick marly layers and discrete bioherms up to several metres thick and 20 m across. Stromatolite columns in the Chandi Formation are 5–10 cm high, sinuous, inclined and straight, with both branched and non-branched types. These stromatolites are composed of calcite micrite and show well defined light and dark laminae with evidence of erosion between lamina sets. The column sinuosity probably originated as a response to changes in direction and strength of currents. Successive flat beds of stromatolite (biostromes), separated by marl/clay horizons, impart a rhythmic pattern to the succession. The Chandi sinuous stromatolite columns resemble those occurring in China, North America and Siberia, of a comparable age, suggesting that similar marine conditions of stromatolite formation might have been operating in the late Mesoproterozoic seas worldwide. However, the petrographic and sedimentological analyses of these stromatolites indicate their development throughin situproduction of carbonate with some trapping and binding of detrital sediment. As a result of the presence of terrigenous material within the stromatolites, whole-rock geochemical analyses for trace elements and rare earth elements cannot be used for interpretation of seawater chemistry and the redox conditions at the time.
Protein kinase A regulation of pigment granule motility in retinal pigment epithelial cells from fish, Lepomis spp.
Authors: Leitner Nicole E. , King-Smith Christina
Published: September 2021

Retinomotor movements include elongation and contraction of rod and cone photoreceptors, and mass migration of melanin-containing pigment granules (melanosomes) of the retinal pigment epithelium (RPE) within the eyes of fish, frogs, and other lower vertebrates. Eyes of these animals do not contain dilatable pupils; therefore the repositioning of the rods and cones and a moveable curtain of pigment granules serve to modulate light intensity within the eye. RPE from sunfish (Lepomis spp.) can be isolated from the eye and dissociated into single cells, allowing in vitro studies of the cytoskeletal and regulatory mechanisms of organelle movement. Pigment granule aggregation from distal tips of apical projections into the cell body can be triggered by the application of underivatized cAMP, and dispersion is effected by cAMP washout in the presence of dopamine. While the phenomenon of cAMP-dependent pigment granule aggregation in isolated RPE was described many years ago, whether cAMP acts through the canonical cAMP-PKA pathway to stimulate motility has never been demonstrated. Here, we show that pharmacological inhibition of PKA blocks pigment granule aggregation, and microinjection of protein kinase A catalytic subunit triggers pigment granule aggregation. Treatment with a cAMP agonist that activates the Rap GEF, Epac (Effector protein activated by cAMP), had no effect on pigment granule position. Taken together, these results confirm that cAMP activates RPE pigment granule motility by the canonical cAMP-PKA pathway. Isolated RPE cells labeled with antibodies against PKA RIIα and against PKA-phosphorylated serine/threonine amino acids show diffuse, punctate labeling throughout the RPE cell body and apical projections. Immunoblotting of RPE lysates using the anti-PKA substrate antibody demonstrated seven prominent bands; two bands in particular at 27 and 64 kD showed increased levels of phosphorylation in the presence of cAMP, indicating their phosphorylation could contribute to the pigment granule aggregation mechanism.
Strictifying and taming directed paths in Higher Dimensional Automata
Authors: Raussen Martin
Published: September 2021

Directed paths have been used by several authors to describe concurrent executions of a program. Spaces of directed paths in an appropriate state space contain executions with all possible legal schedulings. It is interesting to investigate whether one obtains different topological properties of such a space of executions if one restricts attention to schedulings with “nice” properties, e.g. involving synchronisations. This note shows that this is not the case, i.e. that one may operate with nice schedulings without inflicting any harm. Several of the results in this note had previously been obtained by Ziemiański in Ziemiański (2017. Applicable Algebra in Engineering, Communication and Computing28 497–525; 2020a. Journal of Applied and Computational Topology4 (1) 45–78). We attempt to make them accessible for a wider audience by giving an easier proof for these findings by an application of quite elementary results from algebraic topology; notably the nerve lemma.
When neurologists diagnose functional neurological disorder, why don’t they code for it?
Authors: Herbert Lorena DoVal , Kim Rachel , Hassan Asim A.O. , Wilkinson-Smith Alison , Waugh Jeff L.
Published: September 2021

Background

Clinicians who recognize functional neurological disorders (FND) may not share that diagnosis with patients. Poor communication delays treatment and contributes to substantial disability in FND. Diagnostic (ICD-10) coding, one form of medical communication, offers an insight into clinicians’ face-to-face communication. Therefore, quantifying the phenomenon of noncoding, and identifying beliefs and practice habits that reduce coding, may suggest routes to improve medical communication in FND.

Methods

We reviewed all pediatric neurology consultations in our hospital from 2017 to 2020, selecting those in which neurologists explicitly stated an FND-related diagnosis (N = 57). We identified the neurological symptoms and ICD-10 codes assigned for each consultation. In parallel, we reviewed all encounters that utilized FND-related codes to determine whether insurers paid for this care. Finally, we assessed beliefs and practices that influence FND-related coding through a nationwide survey of pediatric neurologists (N = 460).

Results

After diagnosing FND, neurologists selected FND-related ICD-10 codes in only 22.8% of consultations. 96.2% of neurologists estimated that they would code for non-epileptic seizure when substantiated by electroencephalography; in practice, they coded for 36.7% of such consultations. For other FND manifestations, neurologists coded in only 13.3% of cases. When presented with FND and non-FND scenarios with equal levels of information, neurologists coded for FND 41% less often. The strongest predictor of noncoding was the outdated belief that FND is a diagnosis of exclusion. Coding for FND never resulted in insurance nonpayment.

Conclusion

Noncoding for FND is common. Most factors that amplify noncoding also hinder face-to-face communication. Research based on ICD-10 coding (eg, prevalence and cost) may underestimate the impact of FND by >fourfold.