bluuebunny/crossref_metadata_2025 huggingface dataset:
Rapidly Learning Generalizable and Robot-Agnostic Tool-Use Skills for a Wide Range of Tasks
Authors: Qin Meiying , Brawer Jake , Scassellati Brian
Published: December 2021

Many real-world applications require robots to use tools. However, robots lack the skills necessary to learn and perform many essential tool-use tasks. To this end, we present the TRansferrIng Skilled Tool Use Acquired Rapidly (TRI-STAR) framework for task-general robot tool use. TRI-STAR has three primary components: 1) the ability to learn and apply tool-use skills to a wide variety of tasks from a minimal number of training demonstrations, 2) the ability to generalize learned skills to other tools and manipulated objects, and 3) the ability to transfer learned skills to other robots. These capabilities are enabled by TRI-STAR’s task-oriented approach, which identifies and leverages structural task knowledge through the use of our goal-based task taxonomy. We demonstrate this framework with seven tasks that impose distinct requirements on the usages of the tools, six of which were each performed on three physical robots with varying kinematic configurations. Our results demonstrate that TRI-STAR can learn effective tool-use skills from only 20 training demonstrations. In addition, our framework generalizes tool-use skills to morphologically distinct objects and transfers them to new platforms, with minor performance degradation.
The Impact of Scarcity on Pro-environmental Behavior in the COVID-19 Pandemic
Authors: Sachdeva Sonya , Wu James Shyan-Tau , Zhao Jiaying
Published: December 2021

As the world contends with the far-ranging impacts of the COVID-19 pandemic, ongoing environmental crises have, to some extent, been neglected during the pandemic. One reason behind this shift in priorities is the scarcity mindset triggered by the pandemic. Scarcity is the feeling of having less than what is necessary, and it causes people to prioritize immediate short-term needs over long-term ones. Scarcity experienced in the pandemic can reduce the willingness to engage in pro-environmental behavior, leading to environmental degradation that increases the chance of future pandemics. To protect pro-environmental behavior, we argue that it should not be viewed as value-laden and effortful, but rather reconceptualized as actions that address a multitude of human needs including pragmatic actions that conserve resources especially during scarcity. To bolster environmental protection, systematic changes are needed to make pro-environmental behavior better integrated into people's lives, communities, and cities, such that it is more accessible, less costly, and more resilient to future disturbances.
Combining Models of Root-Zone Hydrology and Geoelectrical Measurements: Recent Advances and Future Prospects
Authors: Mary Benjamin , Peruzzo Luca , Iván Veronika , Facca Enrico , Manoli Gabriele , Putti Mario , Camporese Matteo , Wu Yuxin , Cassiani Giorgio
Published: December 2021

Recent advances in measuring and modeling root water uptake along with refined electrical petrophysical models may help fill the existing gap in hydrological root model parametrization. In this paper, we discuss the choices to be made to combine root-zone hydrology and geoelectrical data with the aim of characterizing the active root zone. For each model and observation type we discuss sources of uncertainty and how they are commonly addressed in a stochastic inversion framework. We point out different degrees of integration in the existing hydrogeophysical approaches to parametrize models of root-zone hydrology. This paper aims at giving emphasis to stochastic approaches, in particular to Data Assimilation (DA) schemes, that are generally identified as the best way to combine geoelectrical data with Root Water Uptake (RWU) models. In addition, the study points out a more suitable objective function taken from the optimal transport theory that better captures complex geometry of root systems. Another pathway for improvement of geoelectrical data integration into RWU models using DA relies on the use of stem based methods as a leverage to introduce more extensive root knowledge into RWU macroscopic hydrological models.
Center Pivot Irrigation Systems and Where to Find Them: A Deep Learning Approach to Provide Inputs to Hydrologic and Economic Models
Authors: Cooley Daniel , Maxwell Reed M. , Smith Steven M.
Published: December 2021

Availability and quality of administrative data on irrigation technology varies greatly across jurisdictions. Technology choice, however, will influence the parameters of coupled human-hydrological systems. Equally, changing parameters in the coupled system may drive technology adoption. Here we develop and demonstrate a deep learning approach to locate a particularly important irrigation technology—center pivot irrigation systems—throughout the Ogallala Aquifer. The model does not rely on super computers and thus provides a model for an accessible baseline to train and deploy on other geographies. We further demonstrate that accounting for the technology can improve the insights in both economic and hydrological models.
The Capability of Utilizing Abiotic Enantiomers of Amino Acids by Halomonas sp. LMO_D1 Derived From the Mariana Trench
Authors: Wang Xiangyu , Yang Yi , Lv Yongxin , Xiao Xiang , Zhao Weishu
Published: December 2021

D-amino acids (D-AAs) have been produced both in organisms and in environments via biotic or abiotic processes. However, the existence of these organic materials and associated microbial degradation activity has not been previously investigated in subduction zones where tectonic activities result in the release of hydrothermal organic matter. Here, we isolated the bacterium Halomonas sp. LMO_D1 from a sample obtained from the Mariana trench, and we determined that this isolate utilized 13 different D-AAs (D-Ala, D-Glu, D-Asp, D-Ser, D-Leu, D-Val, D-Tyr, D-Gln, D-Asn, D-Pro, D-Arg, D-Phe, and D-Ile) in the laboratory and could grow on D-AAs under high hydrostatic pressure (HHP). Moreover, the metabolism of L-AAs was more severely impaired under HHP conditions compared with that of their enantiomers. The essential function gene (Chr_2344) required for D-AA catabolism in strain LMO_D1 was identified and confirmed according to the fosmid library method used on the D-AAs plate. The encoded enzyme of this gene (DAADH_2344) was identified as D-amino acid dehydrogenase (DAADH), and this gene product supports the catabolism of a broad range of D-AAs. The ubiquitous distribution of DAADHs within the Mariana Trench sediments suggests that microorganisms that utilize D-AAs are common within these sediments. Our findings provide novel insights into the microbial potential for utilizing abiotic enantiomers of amino acids within the subduction zone of the Mariana trench under HHP, and our results provide an instructive significance for understanding these abiotic enantiomers and allow for insights regarding how organisms within extraterrestrial HHP environments can potentially cope with toxic D-AAs.
Beyond “Women's Traits”: Exploring How Gender, Social Difference, and Household Characteristics Influence Trait Preferences
Authors: Teeken Béla , Garner Elisabeth , Agbona Afolabi , Balogun Ireti , Olaosebikan Olamide , Bello Abolore , Madu Tessy , Okoye Benjamin , Egesi Chiedozie , Kulakow Peter , Tufan Hale Ann
Published: December 2021

Demand-led breeding strategies are gaining importance in public sector breeding globally. While borrowing approaches from the private sector, public sector programs remain mainly focused on food security and social impact related outcomes. This necessitates information on specific user groups and their preferences to build targeted customer and product profiles for informed breeding decisions. A variety of studies have identified gendered trait preferences, but do not systematically analyze differences related to or interactions of gender with other social dimensions, household characteristics, and geographic factors. This study integrates 1000minds survey trait trade-off analysis with the Rural Household Multi-Indicator Survey to study cassava trait preferences in Nigeria related to a major food product, gari. Results build on earlier research demonstrating that women prioritize food product quality traits while men prioritize agronomic traits. We show that food product quality traits are more important for members from food insecure households and gender differences between men and women increase among the food insecure. Furthermore, respondents from poorer households prioritize traits similar to respondents in non-poor households but there are notable trait differences between men and women in poor households. Women in female headed household prioritized quality traits more than women living with a spouse. Important regional differences in trait preferences were also observed. In the South East region, where household use of cassava is important, and connection to larger markets is less developed, quality traits and in ground storability were prioritized more than in other states. These results reinforce the importance of recognizing social difference and the heterogeneity among men and women, and how individual and household characteristics interact to reveal trait preference variability. This information can inform trait prioritization and guide development of breeding products that have higher social impact, which may ultimately serve the more vulnerable and align with development goals.
Biologicals and New Generation Fungicides in the Management of Blast Disease in Rice
Authors: Persaud Rajendra , Saravanakumar Duraisamy , Persaud Mahendra , Seepersad Govind
Published: December 2021

Background: Blast is a devastating disease in rice production. The current research tested the efficacy of biologicals and fungicides against blast. Aqueous extracts of Azadirachta indica, Ocimum basalicum, Cymbopogan flexousus, Thymus vulgaris, Aloe vera, Tagetes patula, Cordia curassavica, Aegle marmelos, Allium fistulosum, Syzygium aromaticum, and Calotropis gigantea were tested in vitro at 5, 10, and 15% concentrations against Magnaporthe oryzae. Also, two isolates, Bacillus cereus OG2L and B. subtilis OG2A, and one isolate, Azotobacter SAG19, and, similarly, five new generation fungicides were evaluated at three different doses against blast pathogen M. oryzae in vitro. The treatments that exhibited the best performance in vitro were further evaluated against blast disease under field conditions.

Results: Extracts of T. patula (5%), C. gigantea (5%), C. curassavica (10%), A. fistulosum (10%), and A. marmelos (15%) showed greater than 81% inhibition to M. oryzae in vitro. Likewise, all three biocontrol agents, viz. B. cereus OG2L, B. subtilis OG2A, and Azotobacter SAG19, demonstrated more than 50% inhibition of mycelial growth of pathogen in vitro, and the commercial formulations of fungicides Propineb, Trifloxystrobin+Tebuconazole, Tebuconazol+Triadimenol, Bacillus subtilis Strain QST 713, and Cinnamon Oil 8%+Clove Oil 2% also significantly inhibited M. oryzae. Under field conditions, C. curassavica (10%), A. marmelos (15%), C. gigantea (5%); B.cereus OG2L; B.subtilis OG2A; Proineb and Trifloxystrobin+Tebuconazole were effective in reducing disease in addition to yield enhancement.

Conclusion: Overall, the plant extracts (C. curassavica at 10%, A. marmelos at 15%, C. gigantea at 5%); bio-agents (B. cereus OG2L, B. subtilis OG2A both at 2g/l), and new generation fungicides Antracol 70WP (Proineb) at 2.5 g/l and Nativo 75 WG (Trifloxystrobin+Tebuconazole) at 0.5 g/l provided effective control against blast disease and superior plant growth and yield compared with other treatments and untreated control.
Analysis of Risk Factors for Non-union After Surgery for Limb Fractures: A Case-Control Study of 669 Subjects
Authors: Quan Kun , Xu Qiang , Zhu Meisong , Liu Xuqiang , Dai Min
Published: December 2021

Objective: The purpose of this study was to analyze the risk factors for limb fracture non-union in order to improve non-union prevention and early detection.

Methods: A total of 223 patients with non-union after surgery for limb fractures performed at our institution from January 2005 to June 2017 were included as the case group, while a computer-generated random list was created to select 446 patients with successful bone healing after surgery for limb fractures who were treated during the same period as the control group, thus achieving a ratio of 1:2. The medical records of these patients were reviewed retrospectively. Age, sex, body mass index, obesity, smoking, alcohol, diabetes, hypertension, osteoporosis, fracture type, multiple fractures, non-steroidal anti-inflammatory drugs (NSAIDs) use, delayed weight bearing, internal fixation failure, and infection data were analyzed and compared between the two groups. A multivariate logistic regression model was constructed to determine relevant factors associated with non-union.

Results: After comparison between two groups by univariate analysis and multivariate logistic regression, we found some risk factors associated that osteoporosis (odds ratio [OR] = 3.16, 95% confidence interval [CI]: 2.05–4.89, p < 0.001), open fracture (OR = 2.71, 95%CI: 1.72–4.27, p < 0.001), NSAIDs use (OR = 2.04, 95%CI: 1.24–3.37, p = 0.005), delayed weight bearing (OR = 1.72, 95%CI: 1.08–2.74, p = 0.023), failed internal fixation (OR = 5.93, 95%CI: 2.85–12.36, p < 0.001), and infection (OR = 6.77, 95%CI: 2.92–15.69, p < 0.001) were independent risk factors for non-union after surgery for limb fractures.

Conclusions: Osteoporosis, open fracture type, NSAIDs use, delayed weight bearing, failed internal fixation, and infection were found to be the main causes of bone non-union; clinicians should, therefore, take targeted measures to intervene in high-risk groups early.
Effect of Changes in Dietary Net Energy Concentration on Growth Performance, Fat Deposition, Skatole Production, and Intestinal Morphology in Immunocastrated Male Pigs
Authors: Batorek-Lukač Nina , Čandek-Potokar Marjeta , Škrlep Martin , Kubale Valentina , Labussière Etienne
Published: December 2021

Nutritional requirements of heavy immunocastrated (IM) pigs and therefore appropriate feeding strategies have not yet been determined. Thus, the effects of changes in dietary net energy (NE) content were studied in 41 IM pigs, fed ad libitum diets with low, medium, and high NE content (LNE, MNE, and HNE diets, with 8.5, 9.3, and 10.0 MJ NE/kg, respectively), from 84 days of age until slaughter at an average age of 172 days and an average body weight of 122.5 kg. In the period from 143 to 170 days of age, there was a tendency for a greater NE intake (p = 0.08) in pigs fed the HNE diet along with greater (p < 0.01) backfat gain. Dietary treatment affected carcass composition, as lower backfat thickness (p = 0.01) and lower area of fat over the longissimus muscle (p = 0.05) were observed in the LNE and MNE pigs. In addition, greater lean meat content (p = 0.04) was observed in the LNE pigs. Reducing the NE of the diet by replacement of cereals and soybean meal with high-fiber ingredients resulted in lower indole production in the ascending colon (p < 0.01) and greater skatole production (p < 0.01) in the cecum. Greater villus area, width, height and perimeter, crypt depth, and thickness of the intestinal mucosa in the jejunum, ileum, ascending colon, and descending colon were found in the LNE group (p < 0.01) than in the HNE group, while those in the MNE group was intermediate. Cell proliferation was not affected by dietary treatment (p > 0.05). The present results show that a reduction in dietary NE concentration lowers lipid deposition, without affecting performance or energy efficiency in IM pigs. This technique provides an advantage in terms of improved leanness, without affecting growth rate in IM pigs after immunization, which is particularly important when the backfat thickness is a determinant of carcass value and IM pigs are fattened to higher weights (e.g., in heavy pig production) or when a longer delay between immunization and slaughter is practiced.
Glutamine Metabolism Scoring Predicts Prognosis and Therapeutic Resistance in Hepatocellular Carcinoma
Authors: Ying Leqian , Cheng Meilian , Lu Yi , Tao Qin , Chen Xiaofeng , Shen Bo , Xiong Fen , Hu Zhangmin , Wang Deqiang , Li Xiaoqin
Published: December 2021

Glutamine metabolism (GM) plays a critical role in hepatocellular carcinoma (HCC); however, a comprehensive methodology to quantify GM activity is still lacking. We developed a transcriptome-based GMScore to evaluate GM activity and investigated the association of GMScore with prognosis and therapeutic resistance. Two independent HCC cohorts with transcriptome data were selected from The Cancer Genome Atlas (TCGA, n = 365) and the International Cancer Genome Consortium (ICGC, n = 231). The expression of 41 GM-associated genes were used to construct and validate GMScore. Several genomic or transcriptomic biomarkers were also estimated. Tumor response to immune checkpoint inhibitors (ICIs) was predicted using the tumor immune dysfunction and exclusion algorithm. GMScore was closely correlated with patient characteristics, including stage, histology grade, alpha-fetoprotein level, and vascular invasion. High GMScore was an independent risk factor for overall survival (OS) in both cohorts (HR = 4.2 and 3.91, both p < 0.001), superior to clinical indices and other biomarkers. High GMScore presented transcriptome features to indicate cell growth advantages and genetic stability, which was associated with poor OS of patients who received transcatheter arterial chemoembolization (TACE). High GMScore was also related to high expression of immune checkpoint genes, increased infiltration of regulatory T cells, and decreased infiltration of M1 macrophages. More importantly, high GMScore indicated poor predicted responses to ICIs, which could be verified in an ICI-treated melanoma cohort. In conclusion, GMScore is a strong prognostic index that may be integrated into existing clinical algorithms. A high GMScore may indicate resistance to TACE and ICIs based on its transcriptome and immune features. Validations using other HCC cohorts, especially ICI-treated HCC cohorts, are necessary.
Binaural Synthetic Aperture Imaging of the Field of Audition as the Head Rotates and Localisation Perception of Monophonic Sound Listened to through Headphones
Authors: Tamsett Duncan
Published: December 2021

A human listening to monophonic sound through headphones perceives the sound to emanate from a point inside the head at the auditory centre at effectively zero range. The extent to which this is predicted by synthetic-aperture calculation performed in response to head rotation is explored. The instantaneous angle between the auditory axis and the acoustic source, lambda, for the zero inter-aural time delay imposed by headphones is 90°. The lambda hyperbolic cone simplifies to the auditory median plane, which intersects a spherical surface centred on the auditory centre, along a prime meridian lambda circle. In a two-dimensional (2-D) synthetic-aperture computation, points of intersection of all lambda circles as the head rotates constitute solutions to the directions to acoustic sources. Geometrically, lambda circles cannot intersect at a point representing the auditory centre; nevertheless, 2-D synthetic aperture images for a pure turn of the head and for a pure lateral tilt yield solutions as pairs of points on opposite sides of the head. These can reasonably be interpreted to be perceived at the sums of the position vectors of the pairs of points on the acoustic image, i.e., at the auditory centre. But, a turn of the head on which a fixed lateral tilt of the auditory axis is concomitant (as in species of owl) yields a 2-D synthetic-aperture image without solution. However, extending a 2-D synthetic aperture calculation to a three-dimensional (3-D) calculation will generate a 3-D acoustic image of the field of audition that robustly yields the expected solution.
Association between Insomnia and Irritable Bowel Syndrome among Adolescents in South Korea: A Quantitative Cross-Sectional Study
Authors: Yang Yun-Yi
Published: December 2021

Insomnia refers to having difficulty sleeping even when given the opportunity and environment to sleep. Irritable bowel syndrome is a functional bowel disease that is accompanied by abdominal pain and irregular defecation but not organic lesions. Although insomnia and irritable bowel syndrome are pathophysiologically related, studies on their relationship among adolescents are lacking. This study aimed to determine the prevalence of insomnia and irritable bowel syndrome among adolescents and the association between them. In May 2021, a survey was conducted among high school adolescents from Daegu Metropolitan City, South Korea, using the Korean version of the Insomnia Severity Index and the Rome IV diagnostic criteria for irritable bowel syndrome. Data were analyzed using chi-square and Mann–Whitney tests and logistic regression analyses. The incidence of insomnia (Insomnia Severity Index score ≥ 10) was 18.4% (n = 74) while that of irritable bowel syndrome was 10.7% (n = 43). Irritable bowel syndrome was strongly associated with an increased incidence of insomnia (odds ratio = 3.30, 95% confidence interval: 1.66–6.53). Therefore, the association between insomnia and irritable bowel syndrome has relevance in the treatment of insomnia in adolescents.
Dynamic Burst Actuation to Enhance the Flow Control Authority of Plasma Actuators
Authors: Ogawa Takuto , Asada Kengo , Sekimoto Satoshi , Tatsukawa Tomoaki , Fujii Kozo
Published: December 2021

A computational study was conducted on flows over an NACA0015 airfoil with dielectric barrier discharge (DBD) plasma. The separated flows were controlled by a DBD plasma actuator installed at the 5% chord position from the leading edge, where operated AC voltage was modulated with the duty cycle not given a priori but dynamically changed based on the flow fluctuations over the airfoil surface. A single-point pressure sensor was installed at the 40% chord position of the airfoil surface and the DBD plasma actuator was activated and deactivated based on the strength of the measured pressure fluctuations. The Reynolds number was set to 63,000 and flows at angles of attack of 12 and 16 degrees were considered. The three-dimensional compressible Navier–Stokes equations including the DBD plasma actuator body force were solved using an implicit large-eddy simulation. Good flow control was observed, and the burst frequency proven to be effective in previous fixed burst frequency studies is automatically realized by this approach. The burst frequency is related to the characteristic pressure fluctuation; our approach was improved based on the findings. This improved approach realizes the effective burst frequency with a lower control cost and is robust to changing the angle of attack.
Low-Rank Coal as a Source of Humic Substances for Soil Amendment and Fertility Management
Authors: Akimbekov Nuraly S. , Digel Ilya , Tastambek Kuanysh T. , Sherelkhan Dinara K. , Jussupova Dariya B. , Altynbay Nazym P.
Published: December 2021

Humic substances (HS), as important environmental components, are essential to soil health and agricultural sustainability. The usage of low-rank coal (LRC) for energy generation has declined considerably due to the growing popularity of renewable energy sources and gas. However, their potential as soil amendment aimed to maintain soil quality and productivity deserves more recognition. LRC, a highly heterogeneous material in nature, contains large quantities of HS and may effectively help to restore the physicochemical, biological, and ecological functionality of soil. Multiple emerging studies support the view that LRC and its derivatives can positively impact the soil microclimate, nutrient status, and organic matter turnover. Moreover, the phytotoxic effects of some pollutants can be reduced by subsequent LRC application. Broad geographical availability, relatively low cost, and good technical applicability of LRC offer the advantage of easy fulfilling soil amendment and conditioner requirements worldwide. This review analyzes and emphasizes the potential of LRC and its numerous forms/combinations for soil amelioration and crop production. A great benefit would be a systematic investment strategy implicating safe utilization and long-term application of LRC for sustainable agricultural production.
A Handheld Grassland Vegetation Monitoring System Based on Multispectral Imaging
Authors: Zhang Aiwu , Hu Shaoxing , Zhang Xizhen , Zhang Taipei , Li Mengnan , Tao Haiyu , Hou Yan
Published: December 2021

Monitoring grassland vegetation growth is of vital importance to scientific grazing and grassland management. People expect to be able to use a portable device, like a mobile phone, to monitor grassland vegetation growth at any time. In this paper, we propose a handheld grassland vegetation monitoring system to achieve the goal of monitoring grassland vegetation growth. The system includes two parts: the hardware unit is a hand-held multispectral imaging tool named ASQ-Discover based on a smartphone, which has six bands (wavelengths)—including three visible bands (450 nm, 550 nm, 650 nm), a red-edge band (750 nm), and two near-infrared bands (850 nm, 960 nm). The imagery data of each band has a size of 5120 × 3840 pixels with 8-bit depth. The software unit improves image quality through vignetting removal, radiometric calibration, and misalignment correction and estimates and analyzes spectral traits of grassland vegetation (Fresh Grass Ratio (FGR), NDVI, NDRE, BNDVI, GNDVI, OSAVI and TGI) that are indicators of vegetation growth in grassland. We introduce the hardware and software unit in detail, and we also experiment in five pastures located in Haiyan County, Qinghai Province. Our experimental results show that the handheld grassland vegetation growth monitoring system has the potential to revolutionize the grassland monitoring that operators can conduct when using a hand-held tool to achieve the tasks of grassland vegetation growth monitoring.
A Comparative Study of the Fertilizer-Cum-Pesticide Effect of Vermicomposts Derived from Cowdung and the Toxic Weed Lantana
Authors: Hussain Naseer , Tabassum-Abbasi  , Abbasi Tasneem , Abbasi Shahid Abbas
Published: December 2021

The effect of vermicomposts, derived either from cowdung or the pernicious invasive plant lantana (Lantana camara), has been assessed on the seed germination, plant growth, fruit yield, quality of the produce, and disease resistance of a common vegetable, ladies finger (Abelmoschus esculentus).Seeds of A. esculentus were germinated and grown in soil fertilized with 0, 2.5, 3.75 and 5 t ha−1 of lantana or cowdung vermicompost for 4 months. It was seen that the lantana vermicompost performed at par or better than the cowdung vermicompost in terms of most of the growth and yield parameters observed. Both the vermicomposts encouraged the germination, growth as well as the yield of ladies fingers. The fruits harvested from the vermicompost-treated plots had greater concentrations of minerals, proteins and carbohydrates than the control plants. Vermicomposts also reduced the incidence of pest attacks on the plants. The results confirm that vermicomposting destroys the harmful ingredients of lantana and turns it into as good a biofertilizer, perhaps even better than the vermicompost of cow-dung. The very large quantities of lantana biomass that is generated in the tropical and sub-tropical regions of the world every year, which presently go to waste, now appear capable of becoming a source of organic fertilizer.
Creep Modelling of Rootstock during Holding in Watermelon Grafting
Authors: Wu Kang , Lou Jianzhong , Li Chen , Luo Wei , Li Congcong , Li Jianping
Published: December 2021

The fragile structure of a rootstock predisposes the stem to mechanical damage during grafting. Thus, it is necessary to take into account the rootstock’s rheological properties under mechanical compression when designing a clamping mechanism. This study focused on cucurbit, a typical rootstock for watermelon grafting. Firstly, we adopted a four-element Burgers model to analyze viscoelastic behavior and deformation characteristics of the rootstock, then conducted creep tests to obtain the parameters of the viscoelastic model. Next, we developed a model for the rootstock during holding based on viscoelastic parameters, loading force and contact time. Moreover, we evaluated the effect of various loading forces and test velocities on creep deformation to reveal the least damage on the rootstock. Results showed that the influence of loading force on the creep deformation was greater than test velocity. Finally, the holding test indicated that the clamping mechanism with silicone rubber can effectively prevent the damage to the stem. Specifically, the loading force should be controlled below 4 N to reduce the associated damage. Taken together, our findings provide a theoretical basis for analyzing the holding damage mechanism during watermelon grafting.
Changes in GHG Emissions Based on Irrigation Water Quality in Short-Term Incubated Agricultural Soil of the North China Plain
Authors: Wang Guangshuai , Du Zhenjie , Ning Huifeng , Liu Hao , Abubakar Sunusi Amin , Gao Yang
Published: December 2021

A worsening water shortage is threatening the sustainable development of agriculture in the North China Plain (NCP). How to make effective use of inferior water resources and alleviate the impact of insufficient water resources on agricultural environments is one of the urgent problems in agricultural production. Although agriculture plays an important role in greenhouse gas (GHG) emissions, the effects of irrigation water quality on such emissions in the NCP are not clear. In this study, we used a short-term incubation experiment to test the effects of the irrigation water quality (underground water (UW), saline water (SW), and reclaimed water (RW)) and frequency (high (H) and low (L)) on regulating the soil GHG emissions of the NCP. The results indicated that RW treatment increased the CO2 and N2O emissions by 15.00% and 20.81%, respectively, and reduced the CH4 uptake by 12.50% compared with the UW treatment. In addition, SW treatment decreased the CO2 and N2O emissions and CH4 uptake by 35.18%, 40.27%, and 20.09% against UW treatment, respectively. The high-frequency water added to the soil significantly increased the GHG emissions for all water qualities applied. Compared with UW, the global warming potential was significantly increased by RW_H and RW_L with 26.48% and 14.5% and decreased by SW_H and SW_L with 32.13% and 43.9%, respectively. Compared with the increase brought by reclaimed water, changing irrigation water sources from conventional groundwater to saline water (4 g L−1) will moderately reduce GHG emissions under the worsening water shortage conditions occurring in the NCP.
Effect of Climate-Smart Agriculture Practices on Climate Change Adaptation, Greenhouse Gas Mitigation and Economic Efficiency of Rice-Wheat System in India
Authors: Kakraliya Suresh K. , Jat Hanuman S. , Sapkota Tek B. , Singh Ishwar , Kakraliya Manish , Gora Manoj K. , Sharma Parbodh C. , Jat Mangi L.
Published: December 2021

Conventional rice–wheat (RW) rotation in the Indo-Gangetic Plains (IGP) of South Asia is tillage, water, energy, and capital intensive. Coupled with these, crop residue burning contributes significantly to greenhouse gas (GHG) emission and environmental pollution. So, to evaluate the GHG mitigation potential of various climate-smart agricultural practices (CSAPs), an on-farm research trial was conducted during 2014–2017 in Karnal, India. Six management scenarios (portfolios of practices), namely, Sc1—business as usual (BAU)/conventional tillage (CT) without residue, Sc2—CT with residue, Sc3—reduced tillage (RT) with residue + recommended dose of fertilizer (RDF), Sc4—RT/zero tillage (ZT) with residue + RDF, Sc5—ZT with residue + RDF + GreenSeeker + Tensiometer, and Sc6—Sc5 + nutrient-expert tool, were included. The global warming potential (GWP) of the RW system under CSAPs (Sc4, Sc5, and Sc6) and the improved BAU (Sc2 and Sc3) were 33–40% and 4–26% lower than BAU (7653 kg CO2 eq./ha/year), respectively. This reflects that CSAPs have the potential to mitigate GWP by ~387 metric tons (Mt) CO2 eq./year from the 13.5 Mha RW system of South Asia. Lower GWP under CSAPs resulted in 36–44% lower emission intensity (383 kg CO2 eq./Mg/year) compared to BAU (642 kg CO2 eq./Mg/year). Meanwhile, the N-factor productivity and eco-efficiency of the RW system under CSAPs were 32–57% and 70–105% higher than BAU, respectively, which reflects that CSAPs are more economically and environmentally sustainable than BAU. The wheat yield obtained under various CSAPs was 0.62 Mg/ha and 0.84 Mg/ha higher than BAU during normal and bad years (extreme weather events), respectively. Thus, it is evident that CSAPs can cope better with climatic extremes than BAU. Therefore, a portfolio of CSAPs should be promoted in RW belts for more adaptation and climate change mitigation.
Diversity and Pathogenicity of Diaporthe Species Revealed from a Survey of Blueberry Orchards in Portugal
Authors: Hilário Sandra , Santos Liliana , Alves Artur
Published: December 2021

Blueberries (Vaccinium corymbosum) are widely cultivated worldwide and largely consumed due to their known antioxidant and medicinal properties. Although Diaporthe species have been documented in Portugal as causal agents of blueberry twig blight and dieback, there is still scarce information on the species that cause these symptoms. Moreover, Diaporthe vaccinii, recently synonymized with D. eres, has been considered a concern to blueberry production worldwide. However, the current knowledge about its impact on blueberries remains unclear. The diversity of Diaporthe species associated with diseased blueberry plants were assessed through a national survey. A multilocus sequence analysis of the rDNA internal transcribed spacer (ITS) region, the translation elongation factor 1-alpha (tef1-α), β-tubulin (tub2), calmodulin (cal) and histone 3 (his3) genes unveiled the presence of Diaporthe ambigua, D. amygdali, D. crousii, D. foeniculina, D. hybrida, D. leucospermi, D. malorum and D. rudis. Moreover, all species were fully characterized based on a detailed morphological description. Diaporthe amygdali, D. hybrida, D. leucospermi and D. malorum are reported for the first time on diseased blueberries in Portugal. Results show that D. eres exhibited a high level of intraspecific variability within isolates, given that the strain CBS 160.32 might be a minor pathogen on blueberry plants, whereas CAA829 was revealed to be the most aggressive. Overall, this study also demonstrates that Diaporthe amygdali and D. eres may be two of the most aggressive species to blueberry plants. This study improves our understanding of the Diaporthe species and it’s causing of dieback and twig blight on Portuguese blueberry orchards. Additionally, the identification of these pathogens represents crucial information for blueberry producers to apply appropriate phytosanitary measures, as well as offering new insights into the potential pathogenicity of D. eres on this host.
Study and Analysis of the Implementation of 4.0 Technologies in the Agri-Food Supply Chain: A State of the Art
Authors: Morella Paula , Lambán María Pilar , Royo Jesús , Sánchez Juan Carlos
Published: December 2021

Industry 4.0 is changing the industrial environment. Particularly, the emerging Industry 4.0 technologies can improve the agri-food supply chain throughout all its stages. This study aims to highlight the benefits of implementing Industry 4.0 in the agri-food supply chain. First, it presents how technologies enhance the agri-food supply chain development. Then, it identifies and highlights the most common challenges that Industry 4.0 implementation faces in agri-food’s environment. After that, it proposes key performance indicators to measure the advantages of this implementation. To achieve this, a systematic literature review was conducted. It combined conceptual and bibliometric analyses of 78 papers. As a result, the most suitable technologies were identified, e.g., Internet of Things, Big Data, blockchain and cyber physical systems. The most used indicators are proposed and the challenges of implementation were detected and classified in three groups, i.e., technical, educational and governmental. This paper highlights and exemplifies the benefits of implementing Industry 4.0 facing the lack of knowledge that exists nowadays. Moreover, it fulfils the gaps in literature, i.e., the lack of information about the implementation of technologies 4.0 or the description of the most relevant indicators for Industry 4.0 implementation.
Genome-Wide Association Study (GWAS) of Mesocotyl Length for Direct Seeding in Rice
Authors: Jang Seong-Gyu , Park So-Yeon , Lar San Mar , Zhang Hongjia , Lee Ah-Rim , Cao Fang-Yuan , Seo Jeonghwan , Ham Tae-Ho , Lee Joohyun , Kwon Soon-Wook
Published: December 2021

Direct seeding is considered an efficient cultivation technology that reduces water use and labor costs. Mesocotyl length is one of the significant traits in cultivation; long mesocotyl is beneficial for the rate and uniformity of seedling emergence. In this study, we used a core collection of 137 rice accessions to identify quantitative trait loci (QTL) for mesocotyl elongation. A genome-wide association study (GWAS), combined with a principal component analysis (PCA) and a kinship matrix analysis, was conducted for the genotype analysis of 2 million, high-quality single nucleotide polymorphisms (SNPs). Through this GWAS analysis, 11 lead SNPs were confirmed to be associated with mesocotyl length, and a linkage disequilibrium (LD) decay analysis identified the 230 kb exploratory range for the detection of QTLs and candidate genes. Based on the gene expression database and haplotype analysis, five candidate genes (Os01g0269800, Os01g0731100, Os08g0136700, Os08g0137800, and Os08g0137900) were detected to be significantly associated with phenotypic variation. Five candidate gene expressions are reported to be associated with various plant hormone responses. Interestingly, two biotic stress response genes and two copper-containing redox proteins were detected as the candidate genes. The results of this study provide associated SNPs in candidate genes for mesocotyl length and strategies for developing direct seeding in breeding programs.
RETRACTED: Montiel-Rozas et al. Effect of Plant Extracts and Metam Sodium on the Soilborne Fungal Pathogens, Meloidogyne spp., and Soil Microbial Community. Agronomy 2020, 10, 513
Authors: Montiel-Rozas María , Díez-Rojo Miguel , Ros Margarita , Pascual José
Published: December 2021

Following publication [...]
Enhancing the Accumulation of Rosavins in Rhodiola rosea L. Plants Grown In Vitro by Precursor Feeding
Authors: Javid Aaqib , Gampe Nóra , Gelana Fulea , György Zsuzsanna
Published: December 2021

Rhodiola rosea produces nearly 150 bioactive compounds. Cinnamyl alcohol glycosides (CAGs) are among the most important secondary metabolites which are specific to this plant species, exhibiting adaptogenic properties along with salidroside. However, raw material supplies for the pharmaceutical industry are hindered by limited access to the plant material. The species is endangered and protected in many areas: cultivation is long and ineffective. Precursor feeding has been found to be an effective strategy for improving the production of secondary metabolites in various plant tissues cultures, including in Rhodiola species. In this study, whole R. rosea plants grown in vitro were subjected to three different precursor treatments, including with trans-cinnamic acid, cinnamaldehyde and cinnamyl alcohol at 2 mM concentrations. The different treatments affected the secondary metabolite production differently. Trans-cinnamic acid did not affect the synthesis significantly, which contradicts earlier studies with cell suspensions. On the other hand, cinnamyl alcohol and cinnamaldehyde were beneficial, improving the production rate of rosin and rosavin by 13.8- and 6.9-fold, and 92.7- and 8.0-fold, respectively. The significant improvement in CAG accumulation due to cinnamaldehyde treatment was unexpected based on previous studies. In addition, cinnamaldehyde triggered the production of rosarin, which the other two treatments failed to do. The study presents the beneficial application of precursors to whole plants grown in vitro.
Tree Growth and Production of Rainfed Valencia Sweet Orange Grafted onto Trifoliate Orange Hybrid Rootstocks under Aw Climate
Authors: Girardi Eduardo Augusto , Ayres Antonio Juliano , Girotto Luiz Fernando , Peña Leandro
Published: December 2021

Brazil is the largest producer of sweet orange and its juice in the world. Extensive cultivated area is located under an Aw climate in the North–Northwest of the state of São Paulo and the Triângulo of Minas Gerais state, being subjected to severe drought events. Although 56% of the orchards are irrigated in these regions, there is a need for drought tolerant rootstocks as an alternative to traditional genotypes such as Rangpur lime and Volkamer lemon, which are susceptible to the endemic citrus sudden death disease (CSD). In this sense, the tree size and production of Valencia sweet orange grafted onto 23 rootstock genotypes were evaluated over a ten-year period in rainfed cultivation at 7.0 m × 3.0 m spacing. Most evaluated types resulted from the cross of Poncirus trifoliata with Citrus, but two interspecific hybrids of Citrus (Sunki mandarin × Rangpur lime hybrids), the Barnes trifoliate orange and a tetraploid selection of Swingle citrumelo were also tested. Tropical Sunki mandarin was used as the reference control. Those hybrids coming from the cross of Sunki × Flying Dragon induced large tree sizes to Valencia sweet orange as well as the other citrandarins, Tropical Sunki mandarin and the Sunki mandarin × Rangpur lime hybrids, whereas only the tetraploid Swingle citrumelo behaved as a dwarfing rootstock, decreasing the canopy volume by 77% compared to that induced by the most vigorous citrandarin 535. The citrandarins 543 and 602 and the citrange C38 induced the highest mean fruit production, 67.2 kg·tree−1, but they also caused pronounced alternate bearing and only the hybrid 543 led to a high production efficiency consistently. Graft incompatibility symptoms were not observed over the evaluation period, and the canopy shape of Valencia sweet orange was also influenced by the rootstocks tested. Two citrandarins and one citrange were selected as the most promising alternative rootstocks for Valencia sweet orange grown under an Aw climate, even though productivity would likely benefit from supplementary irrigation.
Non-Invasive Monitoring of Berry Ripening Using On-the-Go Hyperspectral Imaging in the Vineyard
Authors: Fernández-Novales Juan , Barrio Ignacio , Diago María Paz
Published: December 2021

Hyperspectral imaging offers enormous potential for measuring grape composition with a high degree of representativity, allowing all exposed grapes from the cluster to be examined non-destructively. On-the-go hyperspectral images were acquired using a push broom hyperspectral camera (400–100 nm) that was mounted in the front part of a motorized platform moving at 5 km/h in a commercial Tempranillo vineyard in La Rioja, Spain. Measurements were collected on three dates during grape ripening in 2018 on the east side of the canopy, which was defoliated in the basal fruiting zone. A total of 144 grape clusters were measured for Total soluble solids (TSS), Titratable acidity (TA), pH, Tartaric and Malic acid, Anthocyanins and Total polyphenols, using standard wet chemistry reference methods, throughout the entire experiment. Partial Least Squares (PLS) regression was used to build calibration, cross validation and prediction models for the grape composition parameters. The best performances returned determination coefficients values of external validation (R2p) of 0.82 for TSS, 0.81 for Titratable acidity, 0.61 for pH, 0.62 for Tartaric acid, 0.84 for Malic acid, 0.88 for Anthocyanins and 0.55 for Total polyphenols. The promising results exposed in this work disclosed a notable methodology on-the-go for the non-destructive, in-field assessment of grape quality composition parameters along the ripening period.
Effects of Se Application on Polyamines and Carbon–Nitrogen Metabolism of Pepper Plants Suffering from Cd Toxicity
Authors: Pérez-Millán Rafael , Alfosea-Simón Marina , Simón-Grao Silvia , Cámara-Zapata José María , Zavala-González Ernesto Alejandro , Aranda-Martinez Almudena , Shahid Muhammad Adnan , García-Sánchez Francisco
Published: December 2021

Previous studies have shown that the application of selenium (Se) can efficiently mitigate the toxic effects of cadmium (Cd) on various crops. The objective of the present work is to decipher the mechanisms responsible for the efficiency of Se against the effects of Cd in pepper plants, with respect to the carbon and nitrogen metabolism. The following were analyzed: the concentrations of anions related with this metabolism, such as nitrates, nitrites, and ammonium, the activities of different enzymes such as nitrate reductase, nitrite reductase, and glutamate synthase, polyamines in their different forms, organic acid salts, amino acids, and sugars in the leaf and root tissues of the pepper plants grown in a hydroponics system. Four different treatments were applied: plants without Cd or Se applied (−Cd/−Se); plants grown with Cd added to the nutrient solution (NS) but without Se (+Cd/−Se); plants grown with Cd in the NS, and with the foliar application of Se (+CD/+SeF); and lastly, plants grown with Cd in the NS, and with Se applied to the root (+Cd/+SeR). The metabolites and enzymes related with carbon and nitrogen metabolism were analyzed 15 days after the application. The results showed the superiority of the +Cd/+SeR treatment with respect to the +Cd/+SeF treatment, as shown by an increase in the conjugated polyamines, the decrease in glutamate and phenylalanine, and the increase of malate and chlorogenic acid. The results indicated that SeR decreased the accumulation and toxicity of Se as polyamine homeostasis improved, defense mechanisms such as the phenylpropanoid increased, and the entry of Cd into the plants was blocked.
Questionnaire Background on the Hognose Snake (Heterodon nasicus) Breeders’ Awareness of the Risk of Being Bitten
Authors: Zieliński Damian
Published: December 2021

Several cases of Heterodon nasicus bites producing various symptoms have already been described. In this paper, a survey was conducted among Polish breeders of this species, where 47.7% of the respondents admitted that their snakes had tried to bite them, and 31.4% fed their snakes with their bare hands. When asked whether they feared being bitten by this snake during daily handling, an overwhelming majority of the respondents declared (74% of total negative responses, n = 63) that they were not afraid of being bitten. Based on the popularity of H. nasicus as a pet animal, it can only be assumed that the magnitude of accidental bites is much greater than that reported in published case reports. Therefore, it is important to try to educate pet breeders to report such cases and pay attention to the use of appropriate handling methods during contact with snake food and during feeding.