bluuebunny/crossref_metadata_2025 huggingface dataset:
Açı ve polarizasyondan bağımsız ultra geniş bantlı frekans seçici yüzey tasarımı
Authors: KOCAKAYA Aybike , ÇAKIR Gonca , ÇİMEN Sibel
Published: October 2019

Bu çalışmada,
tek katmanlı, Ultra Geniş Bantlı (UGB), 
açısal kararlı ve polarizasyondan bağımsız özgün bir bant durduran
frekans seçici yüzey (FSY) tasarımı sunulmaktadır. Tasarlanan UGB-FSY’nin birim
hücre geometrisi, halka şeklindeki iletken şerit ile halka açıklıklı çapraz
dipol şeklindeki iletken şeritlerin bir araya getirilmesiyle oluşturulmuştur.
Birim hücre boyutu, UGB bandın en düşük frekansına karşılık gelen serbest uzay
dalga boyu cinsinden 0,07λ × 0,07λ’dır. Önerilen UGB-FSY’nin -3 dB bandı,
Federal İletişim Komisyonu (Federal Communications Commission - FCC) tarafından
tanımlanan UGB bandını kapsayan 3,5 GHz ve 11 GHz frekansları arasındadır.
Birim hücrenin çok küçük olmasından dolayı, hem TE hem de TM modunda 60
dereceye kadar açısal kararlılık göstermektedir. Tasarlanan UGB-FSY’nin
bilgisayar benzetimleri için CST Microwave Studio programı kullanılmıştır.
Benzetim sonuçlarının doğruluğunu test etmek için FSY üretilerek ölçümleri
yapılmıştır. Benzetim sonuçları ile ölçüm sonuçları birbiriyle uyumludur.
Protein Purification for OnePot PURE cell-free system v1
Authors: Ragios Konstantinos
Published: October 2019

In this protocol we explain the procedure of protein purification with a single coculture and a single Ni-NTA affinity His-tag purification to produce the protein solution needed for a PURE cell-free system.
Protein expression in OnePot PURE cell-free system v1
Authors: Ragios Konstantinos
Published: October 2019

In this protocol we explain the procedure of protein expression in the OnePot PURE cell-free system.
Golden Gate lvl 1/2 v1
Authors: Seiler Vinca , Inckemann René
Published: October 2019

Golden Gate reaction protocol for lvl 1/2
Simultaneous fitting of sigmoid curves in Excel (Excel Solver Add-in) v1
Authors: Ohgane Kenji
Published: October 2019

Sometimes it is desirable to fit sigmoid curves with shared parameters against several data sets. Such simultaneous fitting procedure is available in most of the specialized statistical softwares (R, Prism etc.), but also possible with Excel. Here, this protocol explains how to perform simultaneous fitting using Excel Solver plug-in against a pair of data set. For more basic protocol to fit sigmoid curve, see Kenji Ohgane (2019) Sigmoid fitting in Excel (Excel Solver Add-In). protocols.io dx.doi.org/10.17504/protocols.io.78ihrue. See Gerdi Kemmer & Sandro Keller (2010) Nat. Protocols 5: 267–281 for more detail on the use of Excel Solver in nonlinear least square fitting.
Bacteria Staining v1
Authors: Fernández Rodríguez Guillermo
Published: October 2019

A bacteria staining protocol has been automated by OT-2. It allows to check the amount of target we had coated on the 96 well plate. (We used 5 replicates per dilution)
Streptavidin immovilization stress test v2
Authors: Fernández Jorge
Published: October 2019

The following protocol was performed to test the affinity of the protein streptavidin for the nitrocellulose when applied a continuous flow of solvent.
Clinical significance of EGFR mutation subtypes in lung adenocarcinoma : A multi-center Korean study v1
Authors: Yoon Hee-Young , Ryu Jeong-Seon , Kim Dojin , Yong Lee Sung , Choi Juwhan , Ju Ryu Yon , Hwa Lee Jin , Hyun Chang Jung
Published: October 2019

Background: Adenocarcinoma is the most common type of non-small cell lung cancer (NSCLC). Some causative genetic mutations, including deletion of exon (E) 19 and point mutation of E 21, are known to have a favourable prognoses due to sensitivity to tyrosine kinase inhibitors (TKIs); however, the prognoses of other uncommon mutations are unclear. The goal of this study was to analyse the clinical significance of EGFR mutation subtypes in lung adenocarcinoma. Methods: We retrospective reviewed 1021 patients (mean age: 66.8 years, female: 41.7%) who were diagnosed with advanced (stage III–IV) lung adenocarcinoma, had EGFR mutation data, and did not undergo surgery from five medical institutes between 2010–2016. Patients were classified according to EGFR mutation status, particularly for exon-specific mutations. Results: The median follow-up period was 13.3 months. EGFR positivity was 38.1%, with the incidence of mutations in E 18, 19, 20, and 21 being 3.6%, 51.2%, 3.3%, and 41.9%, respectively. The EGFR positive group survived significantly longer than the negative group (p<0.001), and there was a significant difference in survival among the four EGFR subtypes (p=0.004): mutation in E 19 showed a better survival probability compared with the other subtypes (p<0.001), whereas mutation in E 21 showed a poorer prognosis (p=0.006). Multivariate analysis showed that mutation in E 19 was the only significant factor that lowered mortality rate (HR:0.415, p=0.001), while mutations in E 18, 20, and 21 were not significant. Amongst EGFR-positive cases, TKI non-responders had a significantly higher proportion of E 21 mutations compared with TKI-responders (p=0.047). Conclusion: In advanced EGFR-mutant lung adenocarcinoma, mutations in E 19 predict good prognoses, while the presence of mutations in E 18, 20, and 21 did not significantly affect survival. Unlike previous reports, mutations in E 18 and 20 were not worse factors than mutation in E 21.
Cell Counting v2
Authors: Sánchez Laura
Published: October 2019

Bacterial concentration can be measured by several methods. One of them is turbidity. Turbidity, or light scattering, is measured in a spectrophotometer such as a Spectronic 20. This method has the advantage of being the quickest method at the lab bench.
Latex beads behavior assay v1
Authors: Fernandez Mendez Jorge
Published: October 2019

Protocol for testing latex beads agreggation after resuspension in different buffers. It allows determining how beads storage conditions can influence their ability to move freely trhough a membrane.
Aptamer 2-step conjugation protocol (INSA-Lyon 2016) v1
Authors: Fernandez Mendez Jorge
Published: October 2019

Protocol for bioconjugation of amino terminal modified aptamers with carboxyl surface modified latex beads. Adaptated from iGem team INSA-Lyon 2016.
Latex beads migration assay test v2
Authors: Fernandez Mendez Jorge
Published: October 2019

The following protocol details how to test the migration of conjugated latex beads trough different nitrocellulose membranes.
Aptamer conjugated beads affinity assay v1
Authors: Fernandez Mendez Jorge
Published: October 2019

The following protocol details how to test affinity by an aptamer-conjugated particles with their targets, via target immobilization in 96-treated wells.
Streptavidin immovilization stressed test v1
Authors: Fernández Jorge
Published: October 2019

The following protocol was performed to test the affinity of the protein streptavidin for the nitrocellulose when applied a continuous flow of solvent.
The Alliance Formation Puzzle in Contests with Capacity-Constraints: A Test using American Football Reception-Coverage Contest Data v1
Authors: Ehrlich Justin , Chang Yang-Ming , Harmon Matthew , Sanders Shane
Published: October 2019

We utilize a contest-theoretic model to demonstrate a version of the alliance form puzzle that aligns with reception-coverage contests in American football.Namely, secondary defenders can opt for single-coverage (1 v 1 contest) or form a defensive alliance (double-coverage or 2 v 1 contest with exogenous intra-alliance prize division) when defending a given receiver.In our theoretical treatment, we find that defenses have a lower equilibrium success rate in preventing the receiver from “getting open” under double-coverage than under single-coverage in the absence of (sufficiently-binding) capacity constraints.We also find that this success rate paradox is a necessary condition for the alliance formation puzzle (i.e., loss of expected allied party payoffs under alliance).We then test the theoretical treatment by analyzing 8,508 plays of NCAA and NFL game data within a set of fixed effects, logistic regression models that control for receiver, level-of-play, and season-of-play.We find that equilibrium level of defensive success rises significantly (p-value < .01) and substantially (marginal effect of between 12.8 and 17 percentage points) when moving from single-coverage to double-coverage, ceteris paribus.There is strong evidence that the necessary condition for the alliance formation puzzle does not hold in this setting.We conclude that sufficiently-binding (physiological and training-based) capacity constraints eliminate the alliance formation puzzle in this setting (as was shown theoretically by Konrad and Kovenock, 2009).This empirical result suggests that other contest settings that regularly feature alliance (e.g., liquidity-constrained conflict) may not be puzzling.
ChroPlate - ProteinA v2
Authors: Ehl Alexandra , Frommholz David , Stefanczyk Nadine
Published: October 2019

Purification Guide for the Isolation of Antibodies with ChroPlate Filtration Plates by DALEX Biotech. Easy and quick high throughput antibody purification from various sources and species. Each well of the ChroPlate has a binding capacity of > 1 mg (tested with human polyclonal Ig, binding varies between species and clones). The proprietary resin does not shrink or swell in aqueous buffers. High pressure stability. pH stability short term 2 - 8, long term 3 - 8. Excellent thermal stability up to 15 minutes at 80 °C in aqueous buffers at neutral pH. Can be dried for long term storage (80 °C for > 2 h).
HTAPP_ Sorting nuclei into 96-well plates for Smart-Seq2 v1
Authors: Nomura Masashi , Shore Marni , Bussema Lillian , Richman Alyssa , Gritsch Simon , Habib Naomi , Regev Aviv , Suva Mario
Published: October 2019

This protocol describes the process/settings to sort single nuclei into 96-well plates by fluorescence-activated cell sorting (FACS) for Smart-seq2 using the MoFlo Astrios EQ cell sorter (Beckman Coulter). This protocol was applied to frozen pediatric glioma samples for the Human Tumor Atlas Pilot Project (HTAPP). [Note: It does not include instructions on how to operate the cell sorter Beckman Coulter MoFlo Astrios EQ.]
Transformation of Synechocystis sp. PCC 6803 v1
Authors: Wiegard Anika , Maria IM Axmann Ilka
Published: October 2019

This protocol describes how to transform naturally competent Synechocystis sp. PCC 6803. Use this protocol, if you aim at integrating a nucleotide sequence in the Synechocystis chromosome via homologous recombination. Be aware that Synechocystis PCC 6803 strains from different labs vary in their transformation efficiency. The protocol was modified from Grigorieva, G. & Shestakov, S. (1982). Transformation in the cyanobacterium Synechocystis sp. 6803. FEMS Microbiol Lett 13, 367-370.
Processing Bulk Calling Card Sequencing Data v1
Authors: Moudgil Arnav , Mitra Rob
Published: October 2019

Here we present a computational pipeline for processing bulk RNA calling card data. These data will have been generated from transfection/-duction of either undirected piggyBac transposase or your favorite transcription factor (YFTF) fused to piggyBac. Multiple biological replicates should have been generated, each with a unique combination of primer barcode and index sequences. This workflow demonstrates how to analyze a single replicate; the workflow can be parallelized on distributed computing architectures (e.g. slurm).
Unified Dialectical Relations of Terms in English-Chinese Translation
Authors: Mu Huaiyu , Wang Jijun
Published: October 2019

The differences between English and Chinese history and culture lead to the differences in the way of thinking between the two nations, and these differences in the way of thinking naturally reflect in the languages, from which the dialectical relations between English and Chinese arises in some aspects. There is a contradictory relation which is both opposite and unified between common terms in English-Chinese translation. To study the unity of opposites in English-Chinese translation is not to reconcile the contradictions, but to face up to them. Only by knowing what the problems are can we try to find a way to solve them. This paper aims to clarify the origins and definitions of these terms, make a deep analysis to them and then conclude the necessity of their independent existence in translation strategy, method and technique, and at the same time prove that under certain conditions these terms are unified, and that the two sides, which have opposing and unified relations, can also realize the transformation from one side to the other.
May electromagnetic field exposure during pregnancy have a negative effect on anthropometric measurements of the newborn?
Authors: KÖMÜRCÜ KARUSERCİ Özge , ÇÖL Nilgün , DEMİREL Can
Published: October 2019

Purpose: This study was conducted to determine the effect of low frequency electromagnetic field (EMF) exposure due to electronic media device (EMD) during pregnancy on the anthropometric measurements of newborn.Materials and Methods: A questionnaire was administered among randomly selected women offering Gaziantep University, Faculty of Medicine’s Department of Pediatrics Outpatient Clinics for any reason between November 2017-May 2018. Four hundred women who have a child aged between 0-5 years old, completed the questionnaire about their pregnancy history. Results: A negative correlation was identified between maternal single mobile phone usage during pregnancy and newborn’s birth weight. Multiple mobile phone (more than mobile phone) usage during pregnancy was negatively correlated with the birth week, birth weight and birth length of the newborn. The head circumference of the newborn was negatively correlated with watching television (TV) and living close to a base station during pregnancy.Conclusion: Watching TV, mobile phone usage and living near a base station during pregnancy may cause negative effect on the anthropometric measurements of the newborn.
Meşe Palamudunun (Quercus coccifera L.) Farklı Kısımlarının A549, MCF-7 ve HeLa İnsan Kanser Hücrelerine Karşı Antikanser, Antiproliferatif ve Laktat Dehidrojenaz Enzim Aktiviteleri
Authors: GEZİCİ Sevgi
Published: October 2019

Bu çalışmada, Meşe palamudunun (Q.
coccifera) dış-kabuk, külah ve iç kısmından elde edilen
özütlerin; A549, MCF-7, HeLa kanser hücreleri ve kanserli olmayan HUVEC
hücrelerine karşı potansiyel antikanser, antiproliferatif ve nekrotik
kapasiteleri araştırılmıştır. Analizler sonucunda, meşe palamudunun farklı
kısımlarından elde edilen ekstrelerin en düşük konsantrasyonda dahi antikanser
ve antiproliferatif etkiye sahip oldukları ortaya konulmuştur. Palamut
meyvesinin külah kısmından elde edilen etanol özütleri, 5.04±0.02 ile
18.04±0.16 µg/mL arasında değişen IC50 değerleriyle, diğer
kısımlarından elde edilen özütlerden daha yüksek antikanser aktivite
göstermiştir. En yüksek oranda sitotoksik aktivite MCF-7 hücrelerine karşı
gözlenirken; en düşük sitotoksik aktivite ise A549 hücrelerinde gözlenmiştir.
Antikanser ve antiproliferatif aktivite sonuçlarının aksine, en yüksek LDH
aktivitesi HeLa hücrelerinde belirlenmiştir. MTT ve tripan mavisi analiz
sonuçlarıyla uyumlu olarak, en yüksek LDH salınım kapasitesi külah kısmından
elde etanol özütlerinde saptanmıştır. Bu çalışma; meşe palamudunun, kanser
hücrelerinde hücre büyümesini konsantrasyon ve zamana bağlı olarak engelleme
potansiyeline sahip olduğunu ortaya koyan, özgün bir çalışmadır.
Challenges and threats of investigator-initiated multicenter randomized controlled trials: the BACE trial experience
Authors: Vermeersch Kristina , Demeyere Bénédicte , Denaux Karen , De Nys Katelijne , Troosters Thierry , Brusselle Guy G. , Janssens Wim
Published: October 2019

Background: Investigator-initiated clinical research has become a complex environment. Increasing administrative tasks and costs, imposed by stringent regulatory demands, risk to reduce this creative, independent and indispensable research field. The objective of the present study was to illustrate the burden of non-scientific challenges associated with an investigator-initiated multicentre randomized controlled trial, based on the Belgian trial with azithromycin for Chronic obstructive pulmonary disease (COPD) Exacerbations requiring hospitalization (BACE) trial experience.

Methods: The trial enrolled 301 patients with COPD, hospitalized for an acute exacerbation between 2014-2017, and assessed the potential of azithromycin, an off-patent antibiotic. Key experienced challenges were complemented with registry data from the Clinical Trial Centre of the University Hospital Leuven to outline the local clinical respiratory research field, quotations for the trial protocol obtained from 3 pharmaceutical companies to provide insight into the budget restraints and a participation survey to capture the consortium’s perspective.

Results: 60% of the required sample size was enrolled. Key challenges included trial implementation, study drug and database management. Industry-initiated trials dominated the local research field (61%), whereas investigator-initiated prospective interventional and multicenter trials accounted for 19% and 13%, respectively. The triple quotation revealed the BACE trial to require 1.6 to 2.1-fold the amount when executed by the pharmaceutical industry. The survey identified the lack of a local study team as an important obstacle for participation, along with inadequate financial compensation and excessive administrative workload.

Conclusions: Without an adaptation of current regulatory and funding policies to overcome non-scientific challenges, investigator-initiated clinical research is risking to further decline.
Comparison of standard and tubeless percutaneous nephrolithotomy for renal calculi: a prospective randomized control trial
Authors: Kamalakshi Manu Muraleedharan , Ramasamy Vasantharaja , Sadasivan Darsan , Raveendran Sunil , Paramasivam Selvam , Ganapathy Venugopal
Published: October 2019

Background: In the current era of minimally invasive interventions, the mainstay of treatment of renal stones larger than 2 cm is Percutaneous nephrolithotomy (PCNL). PCNL underwent various evolutionary changes minimizing morbidity to the patients. We prospectively compared the outcome of tubeless PCNL (without nephrostomy drainage tube) to reduce the pain and discomfort caused by tube with standard PCNL in the treatment of renal stones.

Methods: In this Randomized control trial (RCT), we divided patients satisfying the inclusion criteria of consenting for trial, single access puncture, less than 3 stones each less than 3 cm, operative duration of less than 2 hours into two groups, standard PCNL (group 1) and tubeless PCNL (group 2) with 25 patients each. Randomization and group assignment were done after complete clearance of renal stones.

Results: Patient’s age, gender, sides of stone and stone size were comparable between two groups (standard versus tubeless PCNL). Postoperative hemoglobin drop, bleeding, pyrexia, urine leak, and blood transfusion requirement did not show a statistically significant difference between the two groups. Analgesic requirement (190 mg versus 80 mg of tramadol), operative duration (49.80 min versus 38.60 min), postoperative pain score (6/10 versus 3.64/10-visual analog scale) and duration of hospital stay (68.48 hours versus 41.12 hours) showed statistically significant difference favoring tubeless PCNL.

Conclusions: Tubeless PCNL may be a safe, acceptable and effective modality of treatment for renal calculi in carefully selected patients comparing standard PCNL resulting in less operative duration, lower postoperative pain, reduced analgesic requirement and shorter hospital stay.
Effectiveness of modified Whitfield’s ointment with oral griseofulvin in the treatment of dermatophytosis poorly responsive to standard antifungal therapy: a randomized, double blind, within patient placebo control
Authors: Fonseka Sanjeewani , Subhani Brabaharan , Kumarasiri P. Vitharana Ranjith
Published: October 2019

Background: Dermatophytosis is a superficial fungal infection found in hot and humid areas particularly in tropical regions and affects the keratinized regions of the body. It is usually treated with a combination of topical and systemic antifungal therapy as well as improved hygienic measures. Over the last few decades there has been an increase in the prevalence of dermatophyte infections which are poorly responding to standard antifungal therapy.

Methods: Modified Whitfield’s ointment is a combination of 5%-5% Salicylic acid and Benzoic acid with an emulsifying ointment as a vehicle which has both a fungistatic and a keratolytic action. Oral Griseofulvin is a systemic antifungal agent which is a fungistatic agent. The combination of the above agents is synergistic. A randomized double blind, within-patient-placebo-controlled trial was designed for the treatment of dermatophytosis poorly responsive to standard antifungal therapy.

Conclusions: This may shed light on the treatment of dermatophytosis poorly responsive to standard antifungal therapy.

Trial Registration: This trial is registered with WHO trial registry number (Universal trial number): U111-1235-8791.

 
Heavy metal resistance properties of bacteria from different soil types in Horo Guduru Wollega, Ethiopia
Authors: Addisu Melkamu T. , Bikila Adugna M.
Published: October 2019

Background: The quality of life on earth is linked inseparably to the overall quality of the environment. Soil pollution with heavy metals has become a critical environmental concern due to its potential adverse ecological effects. The study explored the heavy metals resistance properties of bacteria isolated from fertilizer applied agricultural and non-agricultural soils.

Methods: The soil samples were collected from both fertilizer applied agricultural soils and non-agricultural soils. After identification and characterization of the isolates from both soil types, six (6) similar bacterial isolates were selected to screen for resistance against Cobalt (Co+), Lead (Pb2+), Cromium (Cr+3), Mercury (Hg2+), Nickel (Ni2+), Cadmium (Cd2+) and Zinc (Zn2+) heavy metals. The minimum inhibitory concentration (MIC) for the bacterial isolates were determined by gradually increasing the concentration of heavy metals on agar plates until the isolates failed to show growth.  

Results: The isolates from fertilizer applied agricultural soil showed the highest resistance against the selected heavy metals than those isolated from fertilizers not applied (nonagricultural) soils.

Conclusions: From this result it can be seen that fertilizer has significant role in influencing the heavy metal resistance properties of bacteria and these heavy metal resistant bacteria can be useful for the bioremediation of heavy metal contaminated environment. 
Magnitude of repair failure and associated factors among women undergone obstetric fistula repair in Bahir Dar Hamlin Fistula Center, Amhara Region, Northwest Ethiopia
Authors: Aynie Amare A. , Yihunie Abaynesh G. , Munae Alemtsehay M.
Published: October 2019

Background: Globally, prevention and repair of obstetric fistula remains a challenge, Ethiopia is one of the high-burden countries that constitute to the high fistula belt in the world.

Methods: Cross sectional study was conducted from 1st April 2018 to 1st May 2018 on women who had undergone obstetric fistula repair from 1st January 2013 to 30th December 2017. Systematic sampling technique was applied to select a total of 385 study participants. Data were collected by reviewing clients’ cards using pre-tested questionnaire. Binary and multivariate logistic regression was conducted using SPSS version 21.0 statistical software.  

Results: The magnitude of fistula repair failure was 35.3%. Women weighing <50 kgs (AOR=3.43, 95% CI: 1.89, 6.23), home delivery (AOR=2.40, 95%CI: 1.38, 4.18), labour for >2 days (AOR=3.22, 95%CI: 1.75,5.91), >3 cm width of fistula (AOR=2.30, 95%Cl 1.27, 4.17), grade three fistula (AOR=3.26, 95%CI: 1.29, 8.27), grade four fistula (AOR=9.76, 95%CI: 3.71, 25.67), complete destruction of bladder neck (AOR=2.70, 95%CI: 1.07, 6.66) and post operation infection (AOR=2.98, 95%CI: 1.56, 5.70) were associated factors with fistula repair failure at p value <0.05.

Conclusions: Caution should be taken for obstetric fistula repairing women who presented with less than 50 kg, home delivered, greater than two days labour, greater than 3 cm width of fistula, grade 3 and 4 fistulas, complete destruction of the bladder neck and post-operative infection.
Augmented reality as the medical electronic equipment inventory identifier
Authors: Hermawan Frisa Y. , . Wadianto , Susana Ernia , Ramadani Fauzi , Fauzi Muhammad
Published: October 2019

Background: Inventory systems are very numerous and require a long time when rechecking. In doing a shortcut time for checking, it can be done the application of augmented reality.

Methods: This research can be applied to all types and brands of smartphones. The testing was carried out using three different types and brands of smartphones in three conditions: dark rooms, bright room and open spaces for three times for each smartphone. It examines the suitability of displaying asset objects such as Infusion Device Analyzer (IDA-1), Electrical Safety Analyzer (ISA) and Piranha radiology high voltage tester with target objects using existing inventory labels also Barbarian with the target a book cover with the distance 10, 15, 20, 25 and 30 cm.  

Results: By using three different types and brands of smartphones: A, B and C, the incompatibility result percentage of the asset object with the target object is obtained. The average of incompatibility result of Smartphone A, B, and C for the ISA asset object is 83%. The average of incompatibility result of Smartphone A, B, and C for the IDA asset object is 71%, The average of incompatibility result of Smartphone A, B, and C for the Piranha asset object is 36%, For the whole result for Barbarian object asset is 100%.

Conclusions: Making augmented reality has been successfully carried out with 3D models but there is a discrepancy between the appearance of asset objects with the target object except for the book cover and barbarian.
De novo histoid leprosy in an immunocompetent male: a rare case report from North east India
Authors: Kalvit Kushal R.
Published: October 2019

Histoid leprosy is a rare form of lepromatous (multibacillary) leprosy. This form of leprosy is fairly common in patients on dapsone monotherapy and irregular treatment. Sometimes it can arise de novo as well. Very few cases have been reported in immunocompetent individuals from India. I report a case of de novo histoid leprosy in a 22 year old male from north east India.
Surgical repair of traumatic rupture of superficial and deep digital flexor tendon in a 7 year old working mule with complicated skin injury
Authors: M. S. Abhishek , Prabhat Rohit
Published: October 2019

A 7 year old working mule, with the history of traumatic injury on left hind limb, was presented to TVCC within 6-7 hours of injury with open complicated skin wound. Physical and clinical examination revealed rupture of superficial and deep digital flexor tendons with relative soft tissue and skin damage. The case was diagnosed as traumatic flexor tendon rupture and posted for surgical correction by Bunnell-Mayer suturing technique. Anaesthesia was induced using Xylazine (1.1 mg/kg) and Ketamine (2.2 mg/kg) combination and maintained with thiopental sodium (500 mg) under lateral recumbency. Polyglactin-910 (#2) suture was preferred for tenorrhaphy because of higher tensile and functional strength. Post-operative immobilization was achieved by fiber-glass full limb cast. Animal showed lameness for the first post-operative week, and then slowly reduced. During the cast removal at 6th week post-operatively, there was exuberant granulation tissue formation, which was removed by using cauterization. The dressing of wound was continued with proper debridement and antibiotic till the re-establishment of damaged tissue.
CLINICAL AND ANATOMICAL FORMS OF CONGENITAL CLEFT LIP AND PALATE IN THE REGION WITH ECOTOXICANTS
Authors: Чуйкин Сергей , Chuykin Sergey , Джумартов Назим , Dzhumartov Nazim , Чуйкин Олег , Chuykin Oleg , Кучук Кристина , Kuchuk Kristina , Гринь Эдуард , Grin' Eduard , Чуйкин Георгий , Chuykin Georgy , Муратов Азамат , Muratov Azamat , Гильманов Марсель , Gil'manov Marsel'
Published: October 2019

Subject: the study of the features of clinical and anatomical forms of congenital cleft lip and palate in the region with the petrochemical industry.

Objectives ― determination of the clinical-anatomical forms of congenital cleft lip and palate, the identification of the frequency of severe forms in the region with the petrochemical industry and in the region without industrial eco-toxicants.

Methods. We have studied medical documentation in the Department of Maxillofacial Surgery of the Republican Children's Clinical Hospital for the period from January 1, 1985 to December 31, 2018 to study the structure of the clinical and anatomical forms of congenital clefts of the lips and palate in children living in the Republic of Bashkortostan. During the analyzed period, 3463 children from birth to 16 years with congenital cleft lip and palate underwent treatment and dispensary observation.

Results. Our data suggest that in the area with petrochemical ecotoxicants, the percentage of severe forms of congenital cleft lip and palate is higher (77.5%) than in an area without petrochemical ecotoxicants (68.7%).

Conclusions. Thus, environmental pollution by petrochemical ecotoxicants leads to an increase in the number of children with congenital cleft lip and palate. There is a relationship between the level of air pollution by gross emissions from petrochemical enterprises and the severity of the clinical-anatomical forms of congenital cleft lip and palate in children living in an industrial area. It is important and important in the prenatal prevention of congenital clefts of the lip and palate.
100 años de ascensión a las alturas
Authors: Ortiz Miranda Abraham
Published: October 2019

Son 100 años de historia los que ha evidenciado el país entero al mirar al cielo. Cien años de evolución y desarrollo en los que la Fuerza Aérea Colombiana ha sido inventor de grandes gestas, hazañas éstas que sólo cumplen el único fin de salvaguardar el espacio aéreo colombiano. Sin embargo, tal centenario debe ser visto a través de un foco que permita vislumbrar la completitud y complejidad de la Fuerza a partir de aquéllos grandes hitos que realmente abran la puerta al universo del conocimiento de la misma. Es así que factores tales como el desarrollo tecnológico, la generación de nuevo conocimiento, la versatilidad y flexibilidad presentes en la aplicación del poder aéreo, y la profesionalización y constante formación del personal militar y no uniformado, son algunos de los factores preponderantes para que la Institución Castrense aquí reseñada pueda cumplir con su deber constitucional.

Para tal fin, el presente artículo cuenta con tres secciones en las que se pretenden analizar, someramente, tanto desde la génesis de la Institución como hasta sus tiempos actuales. Dos son los conjuntos de estructuras de desarrollo temporal que han marcado los cien años de la Fuerza, siendo tales dinámicas las que caracterizan los tres acápites de esta reflexión: en un principio, la mencionada entidad castrense se vio advocada a procesos de profesionalización y constante formación de su personal militar y no uniformado para enfrentar múltiples amenazas trasnacionales. Como segundo estadio, tal poder militar aéreo empezó a desarrollar conocimiento novedoso que le ha permitido ampliar sus capacidades institucionales en cuanto a versatilidad y flexibilidad se refiere. Lo anterior, ha tenido gran impacto a nivel internacional en la medida que dichos conglomerados, aún hoy vigentes, han coadyuvado para que esta entidad castrense trascienda las variadas fronteras nacionales; esta aludida labor ha tenido como objetivo el transformar y afianzar sus diferentes formas de relación intersubjetiva, lo cual ha permitido que este organismo del Estado pueda apoyar tanto a distintas naciones del mundo de manera humanitaria como a la investigación científica a la par.
Diseño de la estructura de un cohete de tres etapas para transportar una carga útil de 200 kg a una órbita baja de la Tierra
Authors: Ospina Contreras Daimer , Roldán Torres Luis Carlos
Published: October 2019

El presente artículo muestra el diseño conceptual de la estructura de un cohete de tres etapas capaz de trasportar 200 kg a órbitas bajas de la Tierra, desarrollado en el proyecto de investigación de dos estudiantes de ingeniería aeronáutica de la Fundación Universitaria Los Libertadores. El diseño parte desde la selección de la forma en que se va a formar la estructura para poder resguardar tres motores cohete en su interior teniendo en cuenta la fuerza generada por el primero de ellos ya que esta es la mayor, seguido del diseño de los perfiles que compondrán la estructura y la selección de un material liviano como el aluminio (Al) para soportar esfuerzos de hasta 280 N/mm2, de allí el cálculo de las áreas totales, las áreas requeridas y los momentos de inercia, se inicia con el cálculo del perfil stringer, seguido por el perfil mamparo e incluso el diámetro del remache obteniendo el dimensionamiento, la distribución estructural y la configuración final de la estructura.
STEM Learning Based on Aircraft Design: An Interdisciplinary Strategy Developed to Science Clubs Colombia
Authors: Bravo-Mosquera Pedro David , Cisneros-Insuasti Nelson David , Mosquera-Rivadeneira Fabiola , Avendaño-Uribe Bryann
Published: October 2019

This article describes a STEM learning strategy based on aircraft design in order to promote the development of aeronautical science in Colombia. This teaching strategy was developed by specialized instructors from the Science Clubs Colombia program, seeking to stimulate in young Colombian students their passion for science, technology, and innovation, and in the process, create an international network of academic collaborations. Bloom’s taxonomy was used to classify and select both the educational objectives, as well as the teaching plan of the science club. STEM activities that encourage students to perform hands-on learning experiences were the basis of this framework. Essentially, interdisciplinary activities involving aeronautics, electronics, computational simulations, and technical drawing; characterized this science club. As a result, the students were able to design, manufacture and test their own hand-launched air-model, applying all steps of the scientific method: the conception of ideas, design, execution of experiments, and communication of results. After the first flight tests of the air-models, the students disclosed an ability to apply their mathematics knowledge in conjunction with their science learning on the forces of flight, in order to improve their launching technique. Therefore, both the time and range of the air-models were enhanced. Finally, both students and instructors benefited throughout the learning interaction, since it was the first time that a rural community is the scenario of an aeronautical engineering training process. It is expected that the dissemination of this material will contribute to the Colombian aeronautical community, giving outlooks for new research proposals and cooperation frameworks between government entities and universities.
RİFÂİYYENİN PÎRİ SEYYİD AHMET ER-RİFÂÎ’DE FAKR VE PRATİK TEZÂHÜRÜ FÜTÜVVET
Authors: ÇAKIR Abdullah
Published: October 2019

Tasavvuf
terminolojisinin en önemli kavramlarından biri olan fakr, Seyyid Ahmed er-Rifâî
(ö. 1182)’nin düşüncesinde çok özel bir yer tutar. Fakr kavramı Seyyid Ahmed
er-Rifâî ile birlikte, zühd, fenâ ve kulluk kavramlarını da içerecek bir
vüs’ate kavuşmuştur. Seyyid Ahmed er-Rifâî, Kuran ve sünneti temele alan bir
tasavvufi anlayışı benimsemiş; tasavvufu, sûfiyi ve kulluğu fakr ekseninde
tanımlamış; tasavvuf düşüncesinin varlık, bilgi ve ahlaka dair bakışını fakr
esası üzerine bina etmiştir.  Onun
düşüncesinde fakr sadece hakīkatin elde edildiği derûnî ve manevî bir hal
olmayıp, aynı zamanda sûfiyi Hakk’a, âleme ve insana yönelik sorumluluğa ve
hizmete davet eden aksiyonel bir tezâhürdür. Seyyid Ahmed er-Rifâî’nin tatbîkinde
kendini fütüvvet olarak gösteren bu tezâhür; züll, meskenet, mahviyet ve
acziyet olarak belirmiştir. Diğer taraftan, nefsini tüm varlıktan aşağıda
görme, kınama şeklinde değerlendirilebilecek Melâmî bir neşve olarak tezahür
etmiştir. Bu makalede, sûfîlerin fakr ve fütüvvet kavramlarına dair
görüşlerinin özet bir tasvîri yapılacak, sonrasında ise Seyyid Ahmed
er-Rifâî’nin bu kavramlara nasıl bir yaklaşım sergilediği ele alınacaktır.
17. YÜZYIL MUTASAVVIFLARINDAN KÂDİRÎ MUHYİDDÎN’İN NASÎHATNÂMESİNDE OSMANLI DEVLET İŞLEYİŞİ VE SOSYAL HAYAT
Authors: KAZANCIOĞLU Habibe
Published: October 2019

17.
yüzyıl, Osmanlı Devleti’nin duraklama devrini yaşadığı bir dönemdir. Bu dönemde
siyasî, askerî, sosyal ve ekonomik hayatta büyük değişim ve yozlaşmalar
görülmüştür. Birçok devlet adamı, şâir ve yazarın eserine konu olan bu dönemin
bir mutasavvıf gözüyle değerlendirilmesi önemlidir.Kâdirî Muhyiddîn 17.
yüzyılda yaşamış önemli mutasavvıf ve şâirlerden olup hayatı
hakkında elimizde çok az bilgi bulunmaktadır. Elimizdeki tek eseri Nasîhatnâmesi’dir. Kâdirî
Muhyiddîn İstanbul-Kırım-Kefe şehirleri başta olmak üzere Osmanlı
coğrafyasındaki siyasî, askerî sosyal ve ekonomik
hayatı pek çok açıdan gözlemleme imkânına sahip olmuştur. Bu gözlemlerini 7997
beyitlik manzum nasîhatnâmesinde dile getirmiştir. 17. yüzyılda Osmanlı Devleti’nde
tütün ve kahve kullanımının yaygınlaşması, kahvehânelerin sayısının artması,
zina ve rüşvetin yaygınlaşması, lükse özellikle samur kürklere düşkünlüğün
artması, makamların rüşvetle alınıp satılması, devlet görevlilerinin sürekli
azledilmeleri, meşâyihlerin bozulması ve kendi aralarındaki çekişmeleri,  devlet yönetiminde kadınların ve kötü
musâhiblerin etkili olması, Osmanlı askerî sisteminin bozulması ve çeşitli
tasavvufî konular, Kâdirî Muhyiddîn’in nasîhatnâmesinde
değindiği konulardan bazılarıdır. Bu
çalışmada Kâdirî Muhyiddîn’in gözüyle 17.
yüzyıl Osmanlı devlet işleyişi ve sosyal hayatı ele alınacaktır.
OSMANLI DÖNEMİNDE İMAMLARIN MESLEKİ HAYATLARINDA KARŞILAŞTIKLARI SORUNLAR (18. YÜZYIL DİYARBEKİR EYALETİ ÖRNEĞİ)
Authors: GÜLER Ümit
Published: October 2019

Osmanlı
Devleti’nde imamlar, Müslüman topluma hizmet veren kadrolar içerisinde en geniş
yeri tutmakla birlikte üstlendikleri dinî, toplumsal ve resmi görevlerle
oldukça önemli bir konumdaydılar. Bu sebeple imamlık, Osmanlı İslam toplumunun
önde gelen kurumlarındandır ve geçirdiği değişim ve dönüşümlerle birlikte
günümüzde de büyük bir ehemmiyeti haizdir. Mezkûr öneme istinaden bu çalışmada,
şimdiye kadar herhangi bir araştırmaya konu edilmemiş olan Osmanlı toplumunda
imamların mesleki hayatlarında
karşılaştıkları sorunlar ele alınmıştır. Araştırmanın amacı, 18. yüzyıl
Diyarbekir ahkâm defterleri ve yine Diyarbekir (Âmid) şer’iyye sicilleri
çerçevesinde ilgili dönemde imamların mesleki hayatlarında karşılaştıkları
sorunları ortaya koymaktır. Araştırma neticesinde ilgili dönemde imamların
mesleki hayatlarında karşılaştıkları sorunların toplam 60 farklı belgeye
yansıdığı tespit edilmiştir. Yapılan tasnife göre bu sorunların en sık
rastlanılanı vakıflarla ilgilidir. Bunu sırasıyla usulsüz vergi tahsili meselesi,
görevlerine yönelik usulsüz müdahaleler, nikâh kıyma ve gasil meseleleri ve
sosyal hayatta karşılaşılan az sayıdaki diğer bazı sorunlar takip etmektedir. Bu
sorunlar içerisinde özellikle vakıflar, usulsüz imamlık beratları ve vergilere
dair meselelerin dikkate değer bir ciddiyette olduğu ve ilgili kurumların
işleyişinde birtakım bozulmaların meydana geldiği gözlenmiştir.
Acetabular Fracture Resulting from a Basketball Cutting Movement in a Young Adult Male
Authors: Sperring Thomas R. , Only Arthur J. , Wintermeyer Elke , DeRogatis Michael J. , Issack Paul S.
Published: October 2019

Case:

We present the case of a 26-year-old man who sustained a right transverse-posterior wall acetabular fracture while performing a cutting movement playing basketball.

Conclusions:

Acetabular fracture after a relatively low-energy injury in a healthy young adult male is an extremely unusual event. The cutting movement to the right likely forcefully placed the right hip in flexion, adduction, and internal rotation directing the femoral head into the posterior wall. The patient had excellent clinical and radiographic results after acetabular open reduction and internal fixation.
The Impact of Guided Inquiry Model Integrated with Peer Instruction towards Science Process Skill and Physics Learning Achievement
Authors: Pratiwi Hestingtyas Yuli , Hudha Muhammad Nur , Asri Mathilda , Ahmad Nur Jahan
Published: October 2019

The aim of the research is to find out the difference in science process skill and learning achievement between students who are taught using guided inquiry model integrated with peer instruction and conventional method in VII students in one of the junior high schools in Malang. The method used is quasy-experimental with only post-test group design. The sample was selected using purposive sampling technique, from the 10 population of VII grade students; the researchers selected two classes which were considered to have the same initial skill with total 32 students in each class to be the experimental class and the control class. Instruments used were the observation sheets to measure the science process skill and the test of multiple choices to measure the learning achievement. The researchers gained average score of 81 for science process skill in experimental class and 58 in control class, while  the average score of 77.8 for learning achievement in experimental class and 71.3 in control class as the result of the data analysis. The hypothesis test used T-test, that was the independent sample T-test on Microsoft excel by looking up the score of Ttable on the rate of sig 0.05 for the science process skill and it gained Tcount > Ttable (18.846 > 1.669), while the learning process gained Tcount > Ttable (8.465 > 1.669). It was concluded that the guided inquiry model integrated with peer instruction can improve student’s science process skill and learning achievement compared to conventional method
Increment of plasma glucose by exogenous glucagon is associated with present and future renal function in type 2 diabetes a retrospective study from glucagon stimulation test.
Authors: Takada Yasutaka , Fujita Yukihiro , Bessho Ryoichi , Sato Mao , Abe Tomoe , Yanagimachi Tsuyoshi , Sakagami Hidemitsu , Abiko Atsuko , Takiyama Yumi , Ota Tsuguhito , Haneda Masakazu
Published: October 2019

Background: Glucagon stimulation test (GST) is often employed to assess the insulin reserve of the pancreatic beta cells in diabetic subjects. The clinical significance of the increment of plasma glucose (Δglucose) by exogenous glucagon during GST has not been elucidated. We investigated the relationship between Δglucoseand clinical parameters including the liver and renal function in type 2 diabetic subjects, since we hypothesized that Δglucoseis associated with the liver and renal function reflecting the capacity for gluconeogenesis in the organs.
Methods: A total of 209 subjects with type 2 diabetes who underwent GST during admission were included in this cross-sectional study. We defined the difference between plasma glucose at fasting and 6 min after intravenous injection of 1 mg glucagon as Δglucose. We assessed correlations between Δglucoseand clinical parameters such as diabetic duration, BMI, HbA1c, beta cell function, serum free fatty acids (FFA) which is known to stimulate gluconeogenesis, liver function, the indices of liver function, renal function, and urinary albumin excretion (UAE).
Results: In correlation analysis, Δglucosepositively correlated to FFA and estimated glomerular filtration rate (eGFR), but inversely to serum creatinine and cystatin C, although Δglucoseshowed no correlation with both liver function and the indices of residual liver function. Multiple regression analysis revealed that Δglucose was an independent determinant for the eGFR after 1 year, equally BMI, HbA1c, serum lipids, and UAE, which are known as the predictors for the development of chronic kidney disease.
Conclusion: Our results suggest that Δglucoseduring GST might be related to gluconeogenesis in the kidney and could be the determinant of future renal function in type 2 diabetes.
DOES RELIGIOSITY MATTER IN THE ERA OF INDUSTRIAL REVOLUTION 4.0?
Authors: Ahmad Amri Zainal Adnan  , Nek Kamal Yeop Yunus  , Arsalan Mujahid Ghouri
Published: October 2019

Since the hype of Industrial Revolution 4.0 that was brought up by Klaus Schwab in 2016, once again the question of values and beliefs becomes a debate. If religiosity is sidelined in our efforts to automate services and let machines do the jobs of human, then human will truly be substituted by artificial intelligence (AI). Does religiosity matter and can it play a role in the new industry revolution? This study will explore the impact of religiosity towards Muslim officers’ work behaviour in the higher learning institutions in Malaysia. Through in-depth interviews and thematic analysis, this study found that religiosity has an impact on the respondents’ work behaviour to a certain degree. Five themes emerged from the thematic analysis: religiosity, personality, competency, environment, and leadership. Besides religiosity, factors such as attitude, leadership, and environment also play a vital role in the enhancement of a religious-based organisational culture.
Contained rupture of arch aneurysm managed with total arch, frozen elephant trunk and endograft
Authors: Ibrahim Michael , Assi Roland , McCarthy Fenton , Golden Michael A , Szeto Wilson Y
Published: October 2019

We here describe a complex case of a 75-year-old man presenting with contained rupture of an aortic arch aneurysm in the presence of a second thoracic aortic aneurysm. He was managed with emergent total arch replacement with frozen elephant trunk. Another stent-graft was used to achieve hemostasis at the distal anastomosis. He later underwent TEVAR extension to manage his second aneurysm in a staged fashion. This case demonstrates a number of important concepts in the evolving interaction between open and endovascular therapies of the aortic arch, particularly in the emergent setting.
Apela/Elabela/Toddler: New perspectives in molecular mechanism of heart failure
Authors: Sunjaya Anthony P , Sunjaya Angela F , Ferdinal Frans
Published: October 2019

Background. Despite significant therapeutic advances, heart failure (HF) remains unacceptably high in morbidity and mortality. Additionally, its high-care and costs make HF a deadly and costly disease. First reported independently by two group of researchers, Apela/Elabela/Toddler (ELA) is the second endogenous apelin-receptor ligand discovered which is encoded from a previously classified non-coding gene, and has emerged as a key signalling-pathway in the cardiovascular system.Aims. To explore and summarise the biological effects and diagnostic potential of ELA as a new biomarker for heart failure.Results. ELA (prepro-ELA 54 AA) is a molecule with three isoforms (ELA 11,16 and 32), recently identified as the second endogenous ligand to APJ-receptor and functions to mediate early cardiac development during zebrafish embryogenesis by inducing cardiogenesis, vasculogenesis and bone formation. In adults, it enhances cardiac contractility, promotes vasodilatory effects, mediates fluid homeostasis, reduces food intake, limits kidney dysfunction and exerts anti- atherosclerotic as well as anti-oxidative properties.Conclusion. These results show that ELA, an endogenous agonist of the APJ-receptor exerts cardiovascular effects comparable and potentially more potent than apelin and is found to be downregulated in experimental models and humans with heart failure.