bluuebunny/crossref_metadata_2025 huggingface dataset:
The punctate localisation of the yeast sterol transporter Ysp2p is determined by three dimerisation interfaces in its C-terminus
Authors: Alli-Balogun Ganiyu O. , Ivanović Lazar , Kukulski Wanda , Levine Tim P.
Published: August 2023

Sterol lipids traffic between intracellular compartments by vesicular and non-vesicular routes. Sterol traffic from the plasma membrane to the endoplasmic reticulum (ER), so-called retrograde traffic, particularly depends on a non-vesicular mechanism, being transported by the ubiquitous family ofLipid transfer proteinsAnchored atMembrane contact sites (LAMs, also called GRAMD1/Asters in humans, VASt in plants). LAMs are similar to many lipid transfer proteins in that they localise to membrane contact sites and carry lipids between two organelles. In yeast, the major LAM active at ER-plasma membrane contact sites is Ysp2p, which has a uniquely punctate distribution in the cortical ER. Here, we have comprehensively dissected how Ysp2p achieves its distinctive punctate localisation. We show that the PHGRAMdomain of Ysp2p has membrane binding properties similar to its human counterpart GRAMD1B, but that this is not important for punctate localisation of Ysp2p. Instead, all regions necessary for the punctate localisation of Ysp2p at membrane contacts are present in ∼200 residues at the C-terminus of Ysp2p, with a critical region being a small ý-sheet that we predict homodimerises. We also study the role of punctate localisation of Ysp2 in its function in retrograde sterol traffic, and show that function does not require the punctate localisation, but instead requires a polybasic region adjacent to the sterol transfer domain. Finally, to investigate the interaction of the polybasic region with the plasma membrane, we examine contacts populated by the Ysp2 C-terminus by electron tomography, and find that they consist of generic cortical ER.
Green algae CO2capture is powered by alternative electron pathways of photosynthesis
Authors: Peltier Gilles , Stoffel Carolyne , Findinier Justin , Madireddi Sai Kiran , Dao Ousmane , Epting Virginie , Morin Amélie , Grossman Arthur , Li-Beisson Yonghua , Burlacot Adrien
Published: August 2023

On Earth, microalgae contribute to about half of global net photosynthesis. During photosynthesis, sunlight is converted into chemical energy (ATP and NADPH) used by metabolism to convert CO2into biomass. Alternative electron pathways of photosynthesis have been proposed to generate additional ATP that is required for sustaining CO2fixation, but the relative importance of each pathway remains elusive. Here, we dissect and quantify the contribution of cyclic, pseudo-cyclic and chloroplast to mitochondria electron flows for their ability to sustain net photosynthesis in the microalgaChlamydomonas reinhardtii. We show that each pathway has the potential to energize substantial CO2fixation, can compensate each other, and that the additional energy requirement to fix CO2is more than 3 times higher than previous estimations. We further show that all pathways have very different efficiencies at energizing CO2fixation, with the chloroplast-mitochondria interaction being the most efficient, thus laying bioenergetic foundations for biotechnological improvement of CO2capture.
Germline mitotic quiescence and programmed cell death are induced inC. elegansby exposure to pathogenicP. aeruginosa
Authors: Bollen Daniel P. , Reddy Kirthi C. , Kim Dennis H. , Colaiácovo Monica P.
Published: August 2023

The impact of exposure to microbial pathogens on animal reproductive capacity and germline physiology is not well understood. The nematodeCaenorhabditis elegansis a bacterivore that encounters pathogenic microbes in its natural environment. How pathogenic bacteria affect host reproductive capacity ofC. elegansis not well understood. Here, we show that exposure ofC. eleganshermaphrodites to the Gram-negative pathogenPseudomonas aeruginosacauses a marked reduction in brood size with concomitant reduction in the number of nuclei in the germline and gonad size. We define two processes that are induced that contribute to the decrease in the number of germ cell nuclei. First, we observe that infection withP. aeruginosaleads to the induction of programmed germ cell death. Second, we observe that this exposure induces mitotic quiescence in the proliferative zone of theC. elegansgonad. Importantly, these processes appear to be reversible; when animals are removed from the presence ofP. aeruginosa, germ cell death is abated, germ cell nuclei numbers increase, and brood sizes recover. The reversible germline dynamics during exposure toP. aeruginosamay represent an adaptive response to improve survival of progeny and may serve to facilitate resource allocation that promotes survival during pathogen infection.
Self-tests for COVID-19: what is the evidence? A living systematic review and meta-analysis (2020-2023)
Authors: Anand Apoorva , Vialard Fiorella , Esmail Aliasgar , Khan Faiz Ahmad , O’Byrne Patrick , Routy Jean-Pierre , Dheda Keertan , Pai Nitika Pant
Published: August 2023

COVID-19 self-testing strategy (COVIDST) can rapidly identify symptomatic and asymptomatic SARS-CoV-2-infected individuals and their contacts, potentially reducing transmission. In this living systematic review, we evaluated the evidence for real-world COVIDST performance. Two independent reviewers searched six databases (PubMed, Embase, Web of Science, World Health Organization database, Cochrane COVID-19 registry, Europe PMC) for the period April 1st, 2020, to January 18th, 2023. Data on studies evaluating COVIDST against laboratory-based conventional testing and reported on diagnostic accuracy, feasibility, acceptability, impact, and qualitative outcomes were abstracted. Bivariate random effects meta-analyses of COVIDST accuracy were performed (n=14). Subgroup analyses (by sampling site, symptomatic/asymptomatic infection, supervised/unsupervised strategy, with/without digital supports) were conducted. Data from 70 included studies, conducted across 25 countries with a median sample size of 817 (range: 28-784,707) were pooled. Specificity was high overall, irrespective of subgroups (98.37-99.71%). Highest sensitivities were reported for: a) symptomatic individuals (73.91%, 95%CI: 68.41-78.75%; n=9), b) mid-turbinate nasal samples (77.79%, 95%CI: 56.03-90.59%; n=14), c) supervised strategy (86.67%, 95%CI: 59.64-96.62%; n=13), and d) presence of digital interventions (70.15%, 95%CI: 50.18-84.63%; n=14). Sensitivity was lower in asymptomatic populations (40.18%, 95% CI: 21.52-62.20%; n=4), due to errors in test conduct and absence of supervision or a digital support. We found no difference in COVIDST sensitivity between delta and omicron pre-dominant period. Digital supports increased confidence in COVIDST reporting and interpretation (n=16). Overall acceptability was 91.0-98.7% (n=2) with lower acceptability reported for daily self-testing (39.5-51.1%). Feasibility was 69.0-100.0% (n=5) with lower feasibility (35.9-64.6%) for serial self-testing. COVIDST decreased closures in school, workplace, and social events (n=4). COVIDST is an effective rapid screening strategy for home-, workplace- or school-based screening, for symptomatic persons, and for preventing transmission during outbreaks. This data is useful for updating COVIDST policy. Our review demonstrates that COVIDST has paved the way for the introduction of self-tests, worldwide.
Outsmarting refractory glioblastoma usingin silicoguided, phage display derived peptides targeting oncogenic EGFRvIII
Authors: Kodengil Vettath Sunitha , Loghanathan Malarvizhi Giridharan , Menon Krishnakumar N , Sumitra Vijayachandran Lakshmi
Published: August 2023

EGFRvIII mutation contributes to tumor aggressiveness and poor prognosis in multiple cancers. Despite being tumor-specific, EGFRvIII are not readily detected since they coexist with EGFR. Here, we developed novel, aqueous-dispersible peptides (1-15 kDa) that can detect and actively target EGFRvIII expressing refractory tumors, while sparing wild-type EGFR and normal astrocytes. Based onin silicomolecular modeling, docking and biologics-based approaches, four EGFRvIII-targeting peptides were identified using phage display technology employing a) antibody fragments (AF), and b) random peptide library (RPL). Among RPL-based peptides, RB5 showed appreciable interactions with EGFRvIII receptor, as indicated by binding energy of -10.6 kcal/mol,in silico. In vitrobinding studies using ELISA and immunocytochemistry further confirmed that RB5 could specifically target EGFRvIII+ glioblastoma cell, U87MG.ΔEGFR. Interestingly, AF-based peptide, H13, also showed considerable specificity towards U87MG.ΔEGFR. Importantly, these peptides exerted no cytotoxicity (MTS assay), or affected the downstream phosphorylation (western blot) in EGFRvIII cells. Thus, our study demonstrated that rationally designed peptide molecules can precisely target EGFRvIII tumors with minimal or no intracellular signaling interference. Owing to simple molecular architectures, these non-toxic, aqueous-dispersible peptides hold strong potential for conjugation with antitumor drugs and therapeutic nanoparticles for treating EGFRvIII positive refractory cancers.
Functional recovery associated with dendrite regeneration in PVD neuron ofC. elegans
Authors: Brar Harjot Kaur , Dey Swagata , Singh Pallavi , Pande Devashish , Ghosh-Roy Anindya
Published: August 2023

PVD neuron ofC. elegansis a highly polarized cell with well-defined axonal, and dendritic compartments. PVD neuron operates in multiple sensory modalities controlling both nociceptive touch sensation and body posture. Although both axon and dendrites of this neuron show regeneration response following laser-assisted injury, it is rather unclear how the behavior associated with this neuron is affected by the loss of these structures. It is also unclear whether neurite regrowth would lead to functional restoration in these neurons. Upon axotomy, using a femtosecond laser, we saw that harsh touch response was specifically affected leaving the body posture unperturbed. Subsequently, recovery in the touch response is highly correlated to the axon regrowth, which was dependent on DLK-1 MAP Kinase. Dendrotomy of both major and minor primary dendrites affected the wavelength and amplitude of sinusoidal movement without any apparent effect on harsh touch response. We further correlated the recovery in posture behavior to the type of dendrite regeneration events. We found that dendrite regeneration with the fusion and reconnection between the proximal and distal branches of the injured dendrite corresponded to improved recovery of posture. Our data revealed that the axons and dendrites differentially regulate the functions of PVD neurons. It also revealed that dendrite and axon regeneration are both functionally and molecularly distinct.
Iterative In Silico Screening for Optimizing Stable Conformation of Anti-SARS-CoV-2 Nanobodies
Authors: Shang Wenyuan , Hu Xiujun , Lin Xiaoman , Li Shangru , Xiong Shuchang , Huang BIngding , Wang Xin
Published: August 2023

Nanobodies (Nbs or VHHs) are single-domain antibodies (sdAbs) derived from camelid heavy-chain antibodies. The variable region of these nanobodies has special and unique characteristics, such as small size, good tissue penetration, and cost-effective production, making nanobodies a good candidate for the diagnosis and treatment of viruses. Identifying effective nanobodies against the COVID-19 would help us defeat this dangerous virus or other unknown variants in future. Herein, we introduce an in silico screening strategy for optimizing stable conformation of anti-SARS-CoV-2 nanobodies. Firstly, various complexes containing nanobodies were downloaded from the RCSB database, which were identified from immunized llamas. The primary docking between nanobodies and the SARS-CoV-2 spike protein receptor-binding domain was performed through ClusPro program, with the manually screening that leaving the reasonable conformation to the next step. Then, the binding distances of atoms between the antigen-antibody interfaces were measured through the NeighborSearch algorithm. Finally, filtered nanobodies were acquired according to HADDOCK scores through HADDOCK docking the COVID spike protein with nanobodies under restrictions of calculated molecular distance between active residues and antigenic epitopes less than 4.5 A. In this way, those nanobodies which with more reasonable conformation and with stronger neutralizing efficacy were acquired. To validate the efficacy ranking of the nanobodies we obtained, we calculated the binding affinities and dissociation constants (Kd) of all screened nanobodies using the PRODIGY web tool, and predicted the stability changes induced by all possible point mutations in nanobodies using the MAESTROWeb server. Furthermore, we examined the performance of the relationship between nanobodies' ranking and their number of mutation-sensitive sites (Spearman correlation > 0.68), the results revealed a robust correlation, indicating that the superior nanobodies identified through our screening process exhibited fewer mutation hotspots and higher stability. This correlation analysis demonstrates the validity of our screening criteria, underscoring the suitability of these nanobodies for future development and practical implementation. In conclusion, this three-step screening strategy iteratively in silico greatly improved the accuracy of screening desired nanobodies compared to using only ClusPro docking or default HADDOCK docking settings. It provides new ideas for the screening of novel antibodies and computer-aided screening methods.
Protective effects of Mkl1/2 against lipodystrophy and muscle atrophy via PI3K/AKT-independent FoxO repression
Authors: Kubo Atsushi , Hitachi Keisuke , Shirakawa Ryutaro , Ogura Toshihiko
Published: August 2023

FoxO transcription factors are involved in the pathogenesis of lipodystrophy and muscle atrophy. FoxO proteins are phosphorylated and inactivated by PI3K/AKT signaling; however, little is known about FoxO repressors other than this pathway. Our study showed that the Srf cofactors Mkl1 and Mkl2 directly repressed FoxO transcriptional activity, independent of the PI3K/AKT pathway. Loss ofMkl1/2led to the overactivation of FoxO, which impaired the maintenance of both white adipose tissue (WAT) and skeletal muscle. InMkl2-deficient preadipocytes,Pparγwas suppressed and white adipogenesis was severely impaired. In myotubes, Mkl1 and Mkl2 suppressed the expression of atrophy-related genes (atrogenes) induced by FoxO.Mkl1expression was reduced at the onset of muscle atrophyin vivo, and exogenous supplementation of skeletal muscle Mkl1 suppressed atrogene expression and induced muscle hypertrophy. Finally, both Mkl2 nuclear localization andMkl1/2expression were upregulated by exercise, suggesting thatMkl1/2is involved in the inhibitory effect of exercise on muscle atrophy. These findings indicate that Mkl1/2 act as PI3K/AKT signaling-independent repressors of FoxO and are essential for adipose and muscle tissue homeostasis.
The effects of reference panel perturbations on the accuracy of genotype imputation
Authors: Li Jeremiah H. , Liu Andrew , Buerkle C. Alex , Palmer William , Belbin Gillian M. , Ahangari Mohammad , Gibson Matthew J.S. , Flagel Lex
Published: August 2023

Reference-based genotype imputation is a standard technique that has become increasingly popular in large-scale studies involving genomic data. The two key elements involved in the process of genotype imputation are (1) the haplotype reference panel to which a target individual is being imputed, and (2) the imputation algorithm used to infer missing genotypes in the target individual. The imputation literature has historically focused mainly on (2), with a typical comparative study investigating the relative performance of various imputation algorithms while holding the reference panel constant. However, the role of the reference panel itself (1) on overall imputation performance is equally, if not more, important than the choice among many high-performing algorithms. Even though it is intuitive that the quality of a reference panel should play a role in the accuracy of imputation, it is nonetheless unclear to what extent common errors during panel creation (e.g., genotyping and phase error) lead to suboptimal imputation performance. In this study, we investigate the effects of applying three distinct modes of perturbations to a widely used haplotype reference panel in human genetics on the resulting imputation accuracy. Specifically, we perturb the reference panel by (1) randomly introducing phase errors, (2) randomly introducing genotype errors, and (3) randomly pruning variants from the panel (all at varying magnitudes). We then impute a set of diverse individuals at various sequencing coverages (0.5x, 1.0x, and 2.0x) to these various perturbed panels and evaluate imputation accuracy using ther2metric for the entire cohort as well as ancestry-stratified subsets. We observe that both phase- and genotype-perturbations can dramatically affect imputation accuracy, particularly at very low allele frequencies, while pruning variants has a far smaller effect. We then empirically verified that our simulations reliably predict the impact of potential filtering techniques in a real-world dataset. In the context of haplotype reference panels, these results suggest that phasing and genotyping accuracy are far more important than the density of a reference panel used for imputation.
The SARM1 TIR domain produces glycocyclic ADPR molecules as minor products
Authors: Garb Jeremy , Amitai Gil , Lu Allen , Ofir Gal , Brandis Alexander , Mehlman Tevie , Kranzusch Philip J. , Sorek Rotem
Published: August 2023

Sterile alpha and TIR motif-containing 1 (SARM1) is a protein involved in programmed death of injured axons. Following axon injury or a drug-induced insult, the TIR domain of SARM1 degrades the essential molecule nicotinamide adenine dinucleotide (NAD+), leading to a form of axonal death called Wallerian degeneration. Degradation of NAD+by SARM1 is essential for the Wallerian degeneration process, but accumulating evidence suggest that other activities of SARM1, beyond the mere degradation of NAD+, may be necessary for programmed axonal death. In this study we show that the TIR domains of both human and fruit fly SARM1 produce 1′′–2′ and 1′′–3′ glycocyclic ADP-ribose (gcADPR) molecules as minor products. As previously reported, we observed that SARM1 TIR domains mostly convert NAD+to ADPR (for human SARM1) or cADPR (in the case of SARM1 fromDrosophila melanogaster). However, we now show that human andDrosophilaSARM1 additionally convert ∼0.1–0.5% of NAD+into gcADPR molecules. We find that SARM1 TIR domains produce gcADPR molecules both when purifiedin vitroand when expressed in bacterial cells. Given that gcADPR is a second messenger involved in programmed cell death in bacteria and likely in plants, we propose that gcADPR may play a role in SARM1-induced programmed axonal death in animals.
Female specific dysfunction of sensory neocortical circuits in a mouse model of autism mediated by mGluR5 and Estrogen Receptor α
Authors: Molinaro Gemma , Bowles Jacob E. , Croom Katilynne , Gonzalez Darya , Mirjafary Saba , Birnbaum Shari , Razak Khaleel A. , Gibson Jay R. , Huber Kimberly M.
Published: August 2023

Autism manifests differently in males and females and the brain mechanisms that mediate these sex-dependent differences are unknown. Here, we demonstrate that deletion of the ASD-risk gene,Pten,in neocortical pyramidal neurons (NSEPtenKO) results in robust hyperexcitability of local neocortical circuits in female, but not male, mice, observed as prolonged, spontaneous persistent activity states (UP states). Circuit hyperexcitability in NSEPtenKO mice is mediated by enhanced and/or altered signaling of metabotropic glutamate receptor 5 (mGluR5) and estrogen receptor α (ERα) to ERK and protein synthesis selectively inPtendeleted female neurons. In support of this idea,Ptendeleted Layer 5 cortical neurons have female-specific increases in mGluR5 and mGluR5-driven protein synthesis. In addition, mGluR5-ERα complexes are elevated in female cortex and genetic reduction of ERα inPtenKO cortical neurons rescues circuit excitability, protein synthesis and enlarged neurons selectively in females. Abnormal timing and hyperexcitability of neocortical circuits in female NSEPtenKO mice are associated with deficits in temporal processing of sensory stimuli and social behaviors as well as mGluR5-dependent seizures. Female-specific cortical hyperexcitability and mGluR5-dependent seizures are also observed in a human disease relevant mouse model, germlinePten+/- mice. Our results reveal molecular mechanisms by which sex and a high impact ASD-risk gene interact to affect brain function and behavior.
Vitamin D, calcium and caffeine intake relationship to bone mineral density
Authors: Mubarak Bodour , Ahmed Maisa , Hussein Nahed , Ibrahim Marwa Ezz El-Din
Published: August 2023

Purpose

This study aims to evaluate the caffeinated drinks, levels of vitamin D and calcium in the blood, and their relationship to bone mineral density (BMD) in osteoporotic women in Al-Ahsa Saudi Arabia.

Design/methodology/approach

This study included 85 women over approximately 18 months with ages ranging from 40 to 70 years who were evaluated in the laboratory via blood analysis. Moreover, sociodemographic data and information on health and nutritional awareness were collected via a questionnaire. The BMD was measured by dual-energy X-ray absorptiometry (DXA) and considered osteopenia when the T value total of the lumbar spine or hip was between −1 and −2.5 and osteoporosis < −2.5. Blood levels of vitamin D and calcium were measured via blood analysis.

Findings

There were 54.1% and 45.9% of women who suffered from osteoporosis and low BMD, respectively. There was a significant difference in the number of osteoporotic, and low bone mineral mass groups in marital status (p = 0.04), but no difference was found in age and educational level. A significant difference (p = 0.01) between low bone mass and osteoporosis groups in heights. BMD was significantly and negatively correlated with vitamin D in the blood in individuals with osteoporosis (r = −0.358, P = 0.014). In addition, there was a significant negative correlation between calcium in the blood and total samples (r = −0.355, P = 0.0578). There was a negative significant relationship between calcium supplements and BMD in individuals with low BMD (rt = −0.279, P = 0.041). There was a significant association between cola intake with the occurrence of low BMD (p = 0.027), whereas tea drinking was not associated with risk in both groups.

Originality/value

The study indicated that there is a direct relationship between osteoporosis and low bone mass with different variables. This study was conducted to address the lack of research related to the levels of vitamin D and calcium in the blood and their relationship with BMD in women with low BMD and osteoporosis in Al-Ahsa province-KSA.
What factors influence household financial decisions in Malawi?
Authors: Kachepa Paul , Mumtaz Muhammad Zubair
Published: August 2023

Purpose

This study investigates the factors influencing household financial choices in Malawi. The authors also compare how household financial decisions differ in urban and rural areas.

Design/methodology/approach

The authors utilize the logit model to examine the factors that influence household financial decisions using the Malawi Integrated Household Survey 2019–20, while Oaxaca–Blinder decomposition is used to estimate the variations in household financial decisions between urban and rural areas.

Findings

The authors find that the likelihood of saving increases with income, secondary and tertiary education, and age. The likelihood of saving also decreases with household size and remittances. Additionally, the authors report that marriage reduces the likelihood of loans, whereas sex, age, and income raise the likelihood of loans. According to this study’s findings, income discrepancies between urban and rural samples account for most observed household financial variations. The authors also find that most of the observed variations in household financial decision-making between urban and rural households are reduced when income equality, participation in agriculture, university education, and household size are considered.

Originality/value

Using data from the Malawi Integrated Household Survey 2019–20, this research analyzes the components that affect household financial decisions. While most studies only look at one component of household finances, this study concurrently addresses debt and savings. The study also evaluates whether changes in the variables between urban and rural households impact those households' financing choices.
Dispositional factors enhancing leader–follower relationship's dynamic
Authors: Linando Jaya Addin , Halim M.
Published: August 2023

Purpose

This study investigates dispositional factors' (need for affiliation, positive affectivity and proactive personality) moderation effect on the relationship between leader–follower relationship variables (leader–member exchange and perceived supervisor support) and affective commitment to supervisor.

Design/methodology/approach

In total, 359 employees in Indonesia participated as the study's respondents. This study employs hierarchical regression analysis to test the hypotheses.

Findings

The results show that need for affiliation and positive affectivity moderates the relationship between leader–follower relationship variables and affective commitment to supervisor. In addition, all dispositional factors positively influence affective commitment to supervisor as independent variables. This study's findings depict the social exchange theory in practice.

Originality/value

The present study contributes to theoretical and practical implications. Theoretically, the study extends the knowledge on at least four domains: leader–follower relationship; affective commitment particularly aimed at the supervisor; the roles of dispositional variables on leader–member interactions; and empirically demonstrates social exchange theory. Practically, this study shows which factors are relevant to shaping positive leader–member interactions. Such results are potentially of value for the leader, the organization, and those responsible for recruiting prospective employees.
Standards compliance, participation in the global value chain and the value-added of labour: evidence from Vietnam’s small-and-medium-sized enterprises
Authors: Le Minh Ngoc , Chu Hoang Long
Published: August 2023

Purpose

The authors investigate the impact of standards compliance on the participation in the global value chain and labour value-added of Vietnam’s small and medium-sized enterprises (SMEs).

Design/methodology/approach

The authors use a three-period panel dataset of SMEs combined with Vietnam’s Provincial Competitiveness Index. The authors also use multiple econometric models; and with each model, the authors include all independent variables that are available from the study's data and that are suggested by the literature.

Findings

The authors find that standards compliance by Vietnam’s SMEs improved their participation in the global value chain via subcontracts with FDI multinational firms. The authors also find that standards compliance improved the value-added of labour in Vietnam’s SMEs, which is robust to the choice of econometric models.

Practical implications

The study's results suggest that better outcomes for firms and society will be possible if standards are recognised and respected.

Originality/value

This paper complements scant literature on the impact of standards compliance on global value chain participation via subcontracting work and labour value-added, especially in developing countries.
Start of the work-based learning revolution? The impact on academic quality of Dutch science masters, perceived by university professors
Authors: Grooters Saskia , Zaal Emma
Published: August 2023

Purpose

Work-Based Learning (WBL) is a form of practical learning that is still rare, yet upcoming in academic education. It differs mainly from classical approaches because work experience forms a key curriculum element. To enable WBL, a cultural change in academic landscape seems required, which depends on views and support of academic staff.

Design/methodology/approach

The authors carried out a case study, in which the authors interviewed 20 Dutch science professors to examine their vision on the master track Science, Business and Policy (SBP), a WBL program offered for over 15 years.

Findings

Generally professors are positive about its added value and recommend the track, especially for students with career ambitions outside academia. WBL is perceived to have a positive contribution to academic level. Professors indicated that for academic skills development, compared with a traditional master's program, SBP is evaluated neutral to positive.

Originality/value

Altogether the current climate at the university of Groningen towards WBL seems predominantly positive. The educational change towards a solid inclusion of WBL in the regular curriculum seems supported by one of the key players, the professors. This track could thereby be an example for other WBL programs worldwide.
Work from home and workplace ostracism, beyond the COVID-19 pandemic: moderating effect of perceived organizational support
Authors: Al Riyami Said , Razzak Mohammad Rezaur , Al-Busaidi Adil S.
Published: August 2023

Purpose

This study investigates whether prolonged durations of work from home (WFH) leads to workplace ostracism (WO), and whether such relationship is moderated by perceived organizational support (POS). The context of this research is based on the post-COVID-19 pandemic period, when most organizations have either recalled their employees back to their physical workplaces, or in other cases employees are relegated to continued WFH or to a hybrid model that combines both in-office and remote work. The importance of this study is the spotlight it brings to employees who feel ostracized from their workplace due the continued practice of WFH.

Design/methodology/approach

A conceptual model is developed, by leveraging the conservation of resources (COR) theory. The hypotheses are tested by using cross-sectional survey data collected from 240 employees working in various organizations in the Sultanate of Oman from both public and private sectors. The data are analyzed using R Core Team software.

Findings

The findings of the study reveal that WFH does not have any direct impact on WO. However, when POS is applied as moderator, the results indicate that at low levels of POS, the relationship between WFH and WO becomes significant, but not at moderate to high levels of POS.

Research limitations/implications

This study provides insights into how the phenomenon of WFH is likely to influence perceptions of employees in terms of feeling excluded from the organization by being asked to continue to work remotely, while many of their colleagues have returned to their prepandemic workplaces. The implications of the findings are relevant to the growing literature on employee experiences in the realm of emerging work models being introduced by organizations. Among the limitations of this study is the fact that there may be missing mediators that link WFH with WO, and the possibility that such a study if replicated in other cultural contexts may yield different results.

Practical implications

This study presents evidence to managers on leveraging the power of organizational support to ensure that negative emotions among employees such as WO are mitigated.

Originality/value

This appears to be among the first studies that attempts to provide insights into employee perceptions about WO in the postpandemic period, especially with regards to the emerging work arrangements that are primarily based on WFH that are being widely adopted by many organizations around the world. The results of this study provide useful information about how WFH and POS come together to influence emotions of individuals who have been longing to get back to their normal workplace once the social distancing guidelines of the pandemic were lifted.
Linking production geography and financial performance
Authors: von Dzengelevski Oliver , Netland Torbjørn H. , Vereecke Ann , Ferdows Kasra
Published: August 2023

Purpose

When is it more profitable for multinational manufacturers to manufacture in high-cost environments and when in low-cost environments? While the literature offers many cues to answer this question, too little empirical research directly addresses this. In this study, we quantitatively and empirically investigate the financial effect of companies' production footprint in low-cost and high-cost environments for different types of production networks.

Design/methodology/approach

Using the data of 770 multinational manufacturing companies, we analyze the relationship between production footprints and profitability during four calendar semesters in 2018 and 2019 (N = 2,940), investigating the moderating role of companies' production network type.

Findings

We find that companies with networks distinguished by both high levels of product complexity and process sophistication profit the most from producing to a greater extent in high-cost countries. For these companies, shifting production to low-cost countries would be associated with negative performance implications.

Practical implications

Our findings suggest that the production geography of companies should be attuned to their network type, as defined by the companies' process sophistication and product complexity. Manufacturing in low-cost countries is not always the best choice, as doing so can adversely affect profits if the products are highly innovative and the production processes are complex.

Originality/value

We contribute to the scarce empirical literature on managing global production networks and provide a data-driven analysis that contributes to answering some of the enduring questions in this critical area.
Research on fatigue life evaluation method of shaft parts based on small sample
Authors: Liu Jianhui , Zhang Ziyang , Zhu Longxiang , Wang Jie , He Yingbao
Published: August 2023

Purpose

Due to the limitation of experimental conditions and budget, fatigue data of mechanical components are often scarce in practical engineering, which leads to low reliability of fatigue data and reduces the accuracy of fatigue life prediction. Therefore, this study aims to expand the available fatigue data and verify its reliability, enabling the achievement of life prediction analysis at different stress levels.

Design/methodology/approach

First, the principle of fatigue life probability percentiles consistency and the perturbation optimization technique is used to realize the equivalent conversion of small samples fatigue life test data at different stress levels. Meanwhile, checking failure model by fitting the goodness of fit test and proposing a Monte Carlo method based on the data distribution characteristics and a numerical simulation strategy of directional sampling is used to extend equivalent data. Furthermore, the relationship between effective stress and characteristic life is analyzed using a combination of the Weibull distribution and the Stromeyer equation. An iterative sequence is established to obtain predicted life.

Findings

The TC4–DT titanium alloy is selected to assess the accuracy and reliability of the proposed method and the results show that predicted life obtained with the proposed method is within the double dispersion band, indicating high accuracy.

Originality/value

The purpose of this study is to provide a reference for the expansion of small sample fatigue test data, verification of data reliability and prediction of fatigue life data. In addition, the proposed method provides a theoretical basis for engineering applications.
Does service modularity enhance new service development performance in supply chains: an empirical study
Authors: Osman Osman Mohamed Ali , Jian Zhaoquan
Published: August 2023

Purpose

Customer firms and suppliers are valuable knowledge resources that can be used for achieving superior new service development (NSD) performance. This study aims to investigate how supply chain relationship quality (SCRQ) and knowledge sharing promote the success of NSD, and examines service modularity as an important contingency factor that enhances NSD performance in supply chains.

Design/methodology/approach

Based on service-dominant logic, this study builds a conceptual model to empirically explore the impacts of SCRQ and knowledge sharing on NSD performance, and highlights the moderating effect of service modularity by means of survey methodology of 295 Chinese service firms to test the research hypotheses.

Findings

Regression analysis results show that SCRQ has significant positive effects on knowledge sharing and NSD performance; knowledge sharing plays a partial intermediary role between SCRQ and NSD performance; and service modularity partially moderates the relationships between SCRQ, knowledge sharing and NSD performance.

Research limitations/implications

Generalizations here are limited to Chinese service firms. Service modularity in manufacturing firms experimenting with servitization has yet to be examined and provides a good avenue for future research.

Originality/value

This study contributes to service management literature by providing empirical understanding of how service modularity affects NSD performance in multiprovider contexts. Furthermore, this study offers novel insights on the impacts of inter-firm relationship quality and knowledge sharing in modular collaborative innovation.
A bibliometric and visualization analysis of Islamic fund management research
Authors: Md Husin Maizaitulaidawati , Aziz Shahab , Iqbal Mehwish
Published: August 2023

Purpose

This paper aims to provide bibliometric data from previously published research in Islamic fund management. Several categories, such as the most influential authors, the keywords and themes of the previously published research, were analyzed. Also, this paper provides a rigorous background for Islamic mutual funds research by synthesizing and reviewing the content of the previously published research to identify the gaps and provide future research direction.

Design/methodology/approach

Data were gathered from Scopus databases from the year 2007 until 2022. A total of 115 articles were found published over the period of 15 years. Three different software, namely, R-Studio, VOSviewer and Excel are used to analyze the data that depict, among others, the most impactful authors in the field, the top journals covering Islamic fund management research and the most cited document. Content analysis of the previously published research was also provided.

Findings

Publication in Islamic funds started gaining attention in the year 2012 and after. Collaborative works on Islamic funds are not strong yet among the contributory nations, although the USA and Malaysia contributed the highest number of publications. This study also found that there was a lack of research collaboration among authors in this research field, and most of the articles published were concentrated on the performance measurements of Islamic funds.

Research limitations/implications

Researchers might find the results of this paper useful, as they provide a comprehensive analysis of the previously published research in the field.

Practical implications

While these findings gave an overview of the intellectual structure of the research field, they also have the potential to inspire scholars working on Islamic funds research to collaborate on new research projects. Managers can also use this research to further enhance the emergence of Islamic fund management.

Originality/value

This is a comprehensive paper that examines previously published research in the field of Islamic fund management. The findings of this research benefit practitioners and researchers wishing to embark on research in this niche area.
Analysing SDG disclosure and its impact on integrated thinking and reporting
Authors: Rizzato Fabio , Tonelli Alberto , Fiandrino Simona , Devalle Alain
Published: August 2023

Purpose

The study aims to empirically investigate whether the disclosure of Sustainable Development Goals (SDGs) affects the level of integrated thinking and reporting (ITR) on a sample of European listed companies.

Design/methodology/approach

The sample focusses on companies listed to the STOXX Europe 600 Index. Data have been gathered from Refinitiv DataStream for the period 2019–2020 for the measures of ITR level and SDG disclosure. Then, a multivariate regression analysis is developed to test whether or not, and if so, to what extent, SDG disclosure affects the level of ITR.

Findings

SDG disclosure has been increased over time and companies have primarily focussed on SDG 8, SDG12 and SDG 13 demonstrating their awareness on sustainability issues close to the core business and on the climate urgency. Furthermore, SDG disclosure leads to a higher level of ITR meaning that SDG disclosure is an important pillar contributing to ITR.

Research limitations/implications

The empirical analysis has not deeply investigated each component of ITR and SDG disclosure.

Practical implications

The research can be useful for companies aiming to improve their commitment towards the SDG implementation with an integrated approach. Moreover, the study sheds light on the importance of the SDG disclosure as a determinant of ITR.

Originality/value

The research contributes to literature in the stream of sustainability accounting, by adding new insights on ITR linked to SDG disclosure. To the best of the authors’ knowledge, the originality of the study lies in the inclusion of SDG disclosure as a determinant for ITR that has not been analysed by academics yet.
Examining the immediate and enduring psychological impact of street harassment on women’s mental health
Authors: Thornton Georgina , Willmott Dominic , Richardson Emma , Hudspith Lara
Published: August 2023

Purpose

Many women report experiences of street harassment during their lifetime. Previous quantitative survey research has shown the variety of ways in which this type of harassment can impact upon a victim’s life, including restricting their freedom of movement and fear of further victimisation. The purpose of this study is understand the immediate and enduring psychological impact of street harassment on female victim-survivors.

Design/methodology/approach

The present study aims to explore, qualitatively, women’s experiences of street harassment through thematic analysis of on 35 online blog posts. Data were collected from the “Stop Street Harassment” website, where women are invited to share their experiences anonymously.

Findings

Three main themes were generated from the data. First was the age at which women began to experience street harassment, with recurring early incidents during formative childhood years. Second was the impact that experiences had on their mental health and psychological well-being with feelings of shame, fear, self-loathing, as well as decreased self-esteem and confidence experienced in the immediate aftermath – though the longer-term negative emotions reported were enduring feelings of anger alongside a constant state of anxiety from feelings of vulnerability to further victimisation. The final theme was the modification of behaviour after experiencing street harassment where women choose to avoid walking alone on the streets or consciously changed their clothing choices, to avoid being harassed.

Originality/value

This study offers a further qualitative insight into the real-life experience and psychological consequences of street harassment upon survivors’ mental health.
Family social capital as a mediator between socioemotional wealth and entrepreneurial orientation: evidence from Mexican SMEs
Authors: Ramírez-Solís Edgar Rogelio , Mojarro-Durán Bárbara I , Baños-Monroy Veronica Ilian
Published: August 2023

Purpose

The type of social capital among families involved in business, or family social capital, has both positive and negative effects on family firms. This paper aims to investigate the mediating role of social relationships of family business members between socioemotional wealth (SEW) and firms' entrepreneurial orientation.

Design/methodology/approach

The authors applied a survey conducted in the four main cities in Mexico. The sample consisted of 360 small and medium enterprise (SMEs). This study's research framework and hypothesis were tested using regression analysis and the structural equation modeling technique.

Findings

This study finds that not only does SEW strongly influence the entrepreneurial orientation of family firms, but this influence is also mediated by the capability of such families to develop their social capital.

Research limitations/implications

The results show the perspective of one person in the company. Though it is the person with the highest rank and presumably the person who thoroughly knows the company, there is always a possibility of bias, which may inflate the results presented in this paper.

Practical implications

Based on this study's results, family firms should continuously improve their entrepreneurial abilities to achieve sustainable competitive advantage. In addition, their unique family-related characteristics further enhance these strategic approaches' positive effects on relational capital development.

Originality/value

This work contributes to the academic literature on entrepreneurship and social capital. As a mediator between SEW and entrepreneurial orientation, family relational capital has been under-researched. The results of this study reveal significant implications for networking management and relational capital strategies for SMEs.
Perceived overqualification, work-related boredom, and intention to leave: examining the moderating role of high-performance work systems
Authors: Bochoridou Anna , Gkorezis Panagiotis
Published: August 2023

Purpose

Prior studies have shown various mediating and moderating mechanisms regarding the effect of employees' perceived overqualification on intention to leave (ITL). Nonetheless, only a few empirical studies have shed light on the negative underlying processes that explain this relationship. Furthermore, less is known about the role of high-performance work systems (HPWSs) in the overqualification literature. Drawing upon relative deprivation theory (RDT), this research attempts to fill these gaps by examining the mediating role of work-related boredom and the moderating role of perceived HPWSs in the association between perceived overqualification and ITL.

Design/methodology/approach

Data from a sample of 188 employees working in a Greek manufacturing company were analyzed using the PROCESS macros for SPSS.

Findings

The results indicated that work-related boredom mediates the association between perceived overqualification and ITL. Moreover, HPWSs attenuated the relationship of perceived overqualification with both work-related boredom and ITL, such that their association was positive only when employees' perceptions of HPWSs were low.

Originality/value

This study adds to the existing literature regarding why and how perceived overqualification affects ITL. Even more, this is one of the first studies that examine the role of HPWSs in the literature of overqualification. Theoretical and practical implications were also considered.