bluuebunny/crossref_metadata_2025 huggingface dataset:
Tackling land’s ‘stubborn materiality’: the interplay of imaginaries, data and digital technologies within farmland assetization
Authors: Sippel Sarah Ruth
Published: May 2023

The nature of farming is – still – an essentially biological, and thus volatile, system, which poses substantial challenges to its integration into financialized capitalism. Financial investors often seek stability and predictability of returns that are hardly compatible with agriculture – but which are increasingly seen as achievable through data and digital farming technologies. This paper investigates how farmland investment brokers engage with, perceive, and produce farming data for their investors within a co-constructive process. Tackling land’s ‘stubborn materiality’ for investment, I argue, has material and immaterial components: it includes the re-imagination of farming as a financial asset that delivers reliable income streams for investors; and the re-engineering of farmland’s concrete materialities with digital farming technologies. Farmland investment brokers develop investor-suitable farmland imaginaries, underpinned by storytelling as well as the calculative ‘evidence’ of (digital) data. At the same time, digital technologies have become a key tool for transforming farms into ‘investment grade assets’ endowed with the rich data on farm performance and financial returns requested by investors. I conclude that the assetization and digitization of farmland need to be seen as closely intertwined and mutually reinforcing processes and identify key areas for future research on this intersection.
Innovative system based on natural polyelectrolyte complex and cellulose micro/nanofibers to improve drainability and properties of recycled paper
Authors: Bastida Gabriela A. , Zanuttini Miguel A. , Tarrés Quim , Fiol Núria , Delgado-Aguilar Marc , Galván María V.
Published: May 2023

In this work, the efficiency of a polyelectrolyte complex (PEC) to retain different cellulose micro/nanofibers (CMNFs) during paper formation and to improve the physical properties of recycled unbleached fiber paper was analyzed. CMNFs were obtained from a commercial bleached eucalyptus pulp (BEP) using a PFI refiner followed by a chemical treatment with oxalic acid at two different concentrations. Finally, the pulp was fibrillated using a high-pressure homogenizer at three different intensities. The PEC was formed by addition of the xylan (Xyl) solution on chitosan (CH) solution with a Xyl/CH mass ratio of 80/20. The required dosages of PEC solution to neutralize the charges of different nanocellulose fractions were determined by ζ-potential measurements, and the CMNF retentions on recycled unbleached fibers were evaluated in a Britt Dynamic Drainage Jar. The results showed that the maximum retention was obtained when the neutral PEC-CMNF system was added to pulp. Besides, a significant decrease on °SR was observed when PEC and PEC-CMNF systems were added to the untreated pulp, limiting the negative effects of nanocellulose addition on pulp drainability. The incorporation of PEC-CMNF systems to the handsheets increased the tensile index (up to 28%), Mullen index (up to 40%) and internal bonding (up to 255%). Finally, the compressive strength of the handsheets, namely SCT and CMT, increased up to 30 and 70%, respectively. These simultaneous improvement on drainability and mechanical properties makes the proposed PEC-CMNF system a promising solution for the production of packaging paper.
The Pilgrimage on the Camino de Santiago and Its Impacts on Marital and Familial Relationships: An Exploratory Study
Authors: Tykarski Sławomir , Mróz Franciszek
Published: May 2023

This paper presents the results of a survey conducted among people walking the pilgrimage route to the shrine of St James in Santiago de Compostela. The aim of the research was to investigate how a pilgrimage on the Camino de Santiago among a married couple or family affects marital or familial relationships, whether walking the trail to Compostela together influences the behaviour of family members towards each other and whether it forms positive attitudes and behaviour. The empirical basis of the study is the results of a survey among respondents who made the Camino de Santiago pilgrimage, as well as in-depth interviews conducted with 24 spouses of pilgrims along the Way of St James. The study revealed that doing the pilgrimage as a married couple or family had a positive impact on intra-family relationships. According to the interviewees, going on the pilgrimage together helped to strengthen marital bonds and trust, improve communication and mutual connection, show care and affection and improve contact with children.
ROAD-R: the autonomous driving dataset with logical requirements
Authors: Giunchiglia Eleonora , Stoian Mihaela Cătălina , Khan Salman , Cuzzolin Fabio , Lukasiewicz Thomas
Published: May 2023

Neural networks have proven to be very powerful at computer vision tasks. However, they often exhibit unexpected behaviors, acting against background knowledge about the problem at hand. This calls for models (i) able to learn from requirements expressing such background knowledge, and (ii) guaranteed to be compliant with the requirements themselves. Unfortunately, the development of such models is hampered by the lack of real-world datasets equipped with formally specified requirements. In this paper, we introduce the ROad event Awareness Dataset with logical Requirements (ROAD-R), the first publicly available dataset for autonomous driving with requirements expressed as logical constraints. Given ROAD-R, we show that current state-of-the-art models often violate its logical constraints, and that it is possible to exploit them to create models that (i) have a better performance, and (ii) are guaranteed to be compliant with the requirements themselves.
Life cycle assessment of different strawberry production methods in Germany with a particular focus on plastic emissions
Authors: Galafton Christina , Maga Daniel , Sonnemann Guido , Thonemann Nils
Published: May 2023

Purpose

Using plastic for strawberry cultivation (so-called plasticulture) is widespread to extend cultivation, reduce weed stress and water and nutrient demand, and protect plants. We assess the environmental impacts of different plasticulture methods, including possible impacts caused by the (accidental) emission of plastics. The goals are to help farmers determine the most environmentally friendly cultivation technique and to test the inclusion of plastic pollution in life cycle assessment (LCA).

Methods

An LCA is conducted for a baseline scenario without plasticulture and 11 plasticulture scenarios. The scenarios are modeled using the regionalized ecoinvent 3.8 cut-off database. The functional unit is 1 kg of strawberries of marketable quality at the farm gate produced in the year 2021. The amounts of fertilizer, irrigation, and strawberry output of the scenarios are adapted depending on the plasticulture method based on an extensive literature review. The environmental impacts are assessed using the life cycle impact assessment methods of the Product Environmental Footprint (PEF) recommended by the Joint Research Centre of the European Commission, which is extended to account for plastic-related impacts. The impacts are normalized, weighted, and aggregated, resulting in one single impact score per scenario.

Results and discussion

The scenarios using either a combination of conventional mulch film and row cover or biodegradable mulch film have the lowest total impacts; a macro tunnel or greenhouse causes the highest impacts. There seems to be no trade-off between maximizing productivity and lowering environmental impacts: the scenario with the highest yield also has one of the lowest overall impacts. Only direct emissions are considered regarding plastic pollution. Besides, the plastic pollution impact category is currently limited to the persistence of plastic in the environment and is extendable to but is not yet accounting for exposure and effect of plastic emission on humans and ecosystems.

Conclusion

Plasticulture can increase the yield and reduce environmental impacts for strawberry cultivation compared to open field cultivation. With the used normalization and weighting factors, plastic pollution makes up a considerable share of the overall environmental impact of strawberry cultivation, supporting the recent efforts of different research groups to include plastic emissions in LCA by developing suitable characterization factors.
Machine Learning–Based Prediction of Functional Disability: a Cohort Study of Japanese Older Adults in 2013–2019
Authors: Lu Yongjian , Sato Koryu , Nagai Masato , Miyatake Hirokazu , Kondo Katsunori , Kondo Naoki
Published: May 2023

Background

It is important to identify older adults at high risk of functional disability and to take preventive measures for them at an early stage. To our knowledge, there are no studies that predict functional disability among community-dwelling older adults using machine learning algorithms.

Objective

To construct a model that can predict functional disability over 5 years using basic machine learning algorithms.

Design

A cohort study with a mean follow-up of 5.4 years.

Participants

We used data from the Japan Gerontological Evaluation Study, which involved 73,262 people aged  ≥ 65 years who were not certified as requiring long-term care. The baseline survey was conducted in 2013 in 19 municipalities.

Main Measures

We defined the onset of functional disability as the new certification of needing long-term care that was ascertained by linking participants to public registries of long-term care insurance. All 183 candidate predictors were measured by self-report questionnaires.

Key Results

During the study period, 16,361 (22.3%) participants experienced the onset of functional disability. Among machine learning–based models, ridge regression (C statistic = 0.818) and gradient boosting (0.817) effectively predicted functional disability. In both models, we identified age, self-rated health, variables related to falls and posture stabilization, and diagnoses of Parkinson’s disease and dementia as important features. Additionally, the ridge regression model identified the household characteristics such as the number of members, income, and receiving public assistance as important predictors, while the gradient boosting model selected moderate physical activity and driving. Based on the ridge regression model, we developed a simplified risk score for functional disability, and it also indicated good performance at the cut-off of 6/7 points.

Conclusions

Machine learning–based models showed effective performance prediction over 5 years. Our findings suggest that measuring and adding the variables identified as important features can improve the prediction of functional disability.
Morphological changes and protein degradation during the decomposition process of pig cadavers placed outdoors or in tents—a pilot study
Authors: Geissenberger J. , Amendt J. , Klampfer J. , Thuemmel L. , Jakob L. , Monticelli F. C. , Steinbacher P. , Pittner S.
Published: May 2023

The delimitation of the postmortem interval (PMI) is of utmost importance in forensic science. It is especially difficult to determine the PMI in advanced decomposition stages and/or when dead bodies are found under uncommon circumstances, such as tents, or other (semi-) enclosed environments. In such cases, especially when insect access is restricted, morphological assessment of body decomposition is one of the remaining approaches for delimitation of the PMI. However, as this method allows only vague statements/indications about the PMI, it is required to develop new and more reliable methods. One of the most important candidates is the biochemical analysis of protein degradation. In this regard, it has been demonstrated that specific skeletal muscle protein degradation patterns characterize certain time points postmortem and thus can be used as markers for PMI estimation. In order to test this method in different micro-environments, a pilot study using ten pig carcasses was conducted in summer in Northern Germany. The cadavers were openly placed outside (freely accessible for insects), as well as enclosed in tents nearby, and left to decompose to investigate decomposition processes over a time course of 10 days. Muscle samples of the M. biceps femoris were collected on a regular basis and processed via SDS-PAGE and degradation patterns of selected proteins identified by Western blotting. In addition, morphological changes of the cadavers during decomposition were assessed using the total body score (TBS). Results showed that postmortem protein degradation patterns are largely consistent between treatment groups (open field versus tents) despite major morphological differences in the decomposition rate. This field study provides evidence that muscle protein degradation is mostly unaffected by different levels of exposure, making it a sufficient candidate for PMI delimitation under various circumstances.
Evaluating cancer genetic services in a safety net system: overcoming barriers for a lasting impact beyond the CHARM research project
Authors: Okuyama Sonia , White Larissa L. , Anderson Katherine P. , Medina Elizabeth , Deutsch Sonia , Ransom Chelese , Jackson Paige , Kauffman Tia L. , Mittendorf Kathleen F. , Leo Michael C. , Bulkley Joanna E. , Wilfond Benjamin S. , Goddard Katrina AB , Feigelson Heather Spencer
Published: May 2023

Underserved patients face substantial barriers to receiving cancer genetic services. The Cancer Health Assessments Reaching Many (CHARM) study evaluated ways to increase access to genetic testing for individuals in underserved populations at risk for hereditary cancer syndromes (HCS). Here, we report the successful implementation of CHARM in a low-resource environment and the development of sustainable processes to continue genetic risk assessment in this setting. The research team involved key clinical personnel and patient advisors at Denver Health to provide input on study methods and materials. Through iterative and collaborative stakeholder engagement, the team identified barriers and developed solutions that would both facilitate participation in CHARM and be feasible to implement and sustain long term in clinical care. With a focus on infrastructure building, educational modules were developed to increase awareness among referring providers, and standard methods of identifying and managing HCS patients were implemented in the electronic medical record. Three hundred sixty-four DH patients successfully completed the risk assessment tool within the study, and we observed a sustained increase in referrals to genetics for HCS (from 179 in 2017 to 427 in 2021 post-intervention). Implementation of the CHARM study at a low-resourced safety net health system resulted in sustainable improvements in access to cancer genetic risk assessment and services that continue even after the study ended.

Trial registration NCT03426878
Automatic Classification of Online Discussions and Other Learning Traces to Detect Cognitive Presence
Authors: Dornauer Verena , Netzer Michael , Kaczkó Éva , Norz Lisa-Maria , Ammenwerth Elske
Published: May 2023

Cognitive presence is a core construct of the Community of Inquiry (CoI) framework. It is considered crucial for deep and meaningful online-based learning. CoI-based real-time dashboards visualizing students’ cognitive presence may help instructors to monitor and support students’ learning progress. Such real-time classifiers are often based on the linguistic analysis of the content of posts made by students. It is unclear whether these classifiers could be improved by considering other learning traces, such as files attached to students’ posts. We aimed to develop a German-language cognitive presence classifier that includes linguistic analysis using the Linguistic Inquiry and Word Count (LIWC) tool and other learning traces based on 1,521 manually coded meaningful units from an online-based university course. As learning traces, we included not only the linguistic features from the LIWC tool, but also features such as attaching files to a post, tagging, or using terms from the course glossary. We used the k-nearest neighbor method, a random forest model, and a multilayer perceptron as classifiers. The results showed an accuracy of up to 82% and a Cohen’s κ of 0.76 for the cognitive presence classifier for German posts. Including learning traces did not improve the predictive ability. In conclusion, we developed an automatic classifier for German-language courses based on a linguistic analysis of students’ posts. This classifier is a step toward a teacher dashboard. Our work also provides the first fully CoI-coded German dataset for future research on cognitive presence.
Fundamental Understanding and Optimization Strategies for Dual-Ion Batteries: A Review
Authors: Chen Chong , Lee Chun-Sing , Tang Yongbing
Published: May 2023

There has been increasing demand for high-energy density and long-cycle life rechargeable batteries to satisfy the ever-growing requirements for next-generation energy storage systems. Among all available candidates, dual-ion batteries (DIBs) have drawn tremendous attention in the past few years from both academic and industrial battery communities because of their fascinating advantages of high working voltage, excellent safety, and environmental friendliness. However, the dynamic imbalance between the electrodes and the mismatch of traditional electrolyte systems remain elusive. To fully employ the advantages of DIBs, the overall optimization of anode materials, cathode materials, and compatible electrolyte systems is urgently needed. Here, we review the development history and the reaction mechanisms involved in DIBs. Afterward, the optimization strategies toward DIB materials and electrolytes are highlighted. In addition, their energy-related applications are also provided. Lastly, the research challenges and possible development directions of DIBs are outlined.
Evidence for Targeted and Universal Secondary School-Based Programs for Anxiety and Depression: An Overview of Systematic Reviews
Authors: Zbukvic Isabel , McKay Samuel , Cooke Samantha , Anderson Rebekah , Pilkington Vita , McGillivray Lauren , Bailey Alan , Purcell Rosemary , Tye Michelle
Published: May 2023

While there are a number of systematic reviews on school-based mental health programs, there appears to be heterogeneity in their overall findings and conclusions, possibly due to the tendency to combine evidence from primary school and high school programs. To investigate the evidence for the effectiveness of targeted (for specific groups) and universal (for all students) mental health programs delivered in secondary schools, a systematic review of systematic reviews was conducted. A systematic search for reviews published from 2015 included outcomes for depression and anxiety—the most common mental health conditions—and quality appraisal of original studies in majority secondary school settings. A total of 395 references were screened and 14 systematic reviews were included. Of reviews that were assessed in full, most were excluded for not clearly separating studies in secondary school settings from primary school settings. Findings were generally positive but heterogenous in terms of quality and long-term outcomes. Interventions were mainly based on cognitive behavioural therapy (CBT), with results showing that targeted interventions are generally effective in the short-term for both anxiety and depression, while universal programs may be effective in some situations, typically in the short term and when programs were CBT-based; where reviews focused on universal resilience programs there were no significant effects. The overview shows a need for systematic reviews focused on secondary school settings, which consider contextual and individual factors that can influence the implementation and effectiveness of programs.
The IHI Rochester Report 2022 on Healthcare Informatics Research: Resuming After the CoViD-19
Authors: Combi Carlo , Facelli Julio C. , Haddawy Peter , Holmes John H. , Koch Sabine , Liu Hongfang , Meyer Jochen , Peleg Mor , Pozzi Giuseppe , Stiglic Gregor , Veltri Pierangelo , Yang Christopher C.
Published: May 2023

In 2020, the pandemic spread worldwide in an unexpected way and suddenly modified many life issues, including social habits, social relationships, teaching modalities, and more. Such changes were also observable in many different healthcare and medical contexts. Moreover, the pandemic acted as a stress test for many research endeavors, and revealed some limitations, especially in contexts where research results had an immediate impact on the social and healthcare habits of millions of people. As a result, the research community is called to perform a deep analysis of the steps already taken, and to re-think steps for the near and far future to capitalize on the lessons learned due to the pandemic. In this direction, on June 09th–11th, 2022, a group of twelve healthcare informatics researchers met in Rochester, MN, USA. This meeting was initiated by the Institute for Healthcare Informatics—, and hosted by the Mayo Clinic. The goal of the meeting was to discuss and propose a research agenda for biomedical and health informatics for the next decade, in light of the changes and the lessons learned from the pandemic. This article reports the main topics discussed and the conclusions reached. The intended readers of this paper, besides the biomedical and health informatics research community, are all those stakeholders in academia, industry, and government, who could benefit from the new research findings in biomedical and health informatics research. Indeed, research directions and social and policy implications are the main focus of the research agenda we propose, according to three levels: the care of individuals, the healthcare system view, and the population view.
Role of Lp(a) in patients with erectile dysfunction undergoing angioplasty for symptomatic pelvic artery disease
Authors: Kalka Christoph , Lippik Lisa , Wenzel Folker , Hoppe Hanno , Keo Hak-Hong , Heiss Christian , Diehm Nicolas
Published: May 2023

Summary: Background: Atherosclerotic disease of erection-related arteries is a major reason for erectile dysfunction (ED). Lp(a) has been implied in the pathophysiology of atherosclerosis in the coronary and lower limb arteries. Here, we investigated if Lp(a) plays a specific role in ED due with symptomatic pelvic artery atherosclerosis. Patients and methods: Out of 276 consecutive patients treated for ED with angioplasties on proximal (69%) and distal (31%, distal to Alcock channel) erection-related arteries, 236 patients (age: 62±10 years) of which Lp(a) values were available were retrospectively analyzed. Results: The baseline International Index of Erectile Function-15 (IIEF-15) score was 29±15 and significantly increased to 43±20 (increase: 14±21) after treatment at average follow up of 286±201 days. In 25%, Lp(a) values were elevated to more than 30 mg/dL. Hypercholesterolemia, coronary, lower extremity peripheral, and polyvascular disease were more common in patients with Lp(a) ≥60 mg/dl. Anatomic arterial lesion distribution (proximal/distal), improvement in IIEF-15 and clinically driven re-intervention rate (overall 7%) did not differ between patients with <30, 30–59, and ≥60 mg/dL Lp(a). Conclusions: While angioplasty is an effective therapy for ED of arterial origin in patients with obstruction of erection-related arteries, Lp(a) does not seem to play a major role for clinical outcomes in these patients.
Improved 3D Shallow‐Deep Vs Structure in Tongzhou, Beijing (China), Revealed by Dense Array Ambient Noise Tomography
Authors: Xu Wei , Ding Zhifeng , Wu Pingping , Lu Laiyu , Qin Tongwei
Published: May 2023

Shear‐wave velocity (Vs) structures can reveal the shallow sediment thickness and deep tectonic features of buried faults and geological units. They are important for reducing seismic and geological disasters in urban areas. Based on ambient noise data from the Tongzhou dense array (919 seismographic stations), we obtain a fine shallow‐deep (0–5 km) 3D Vs model, by jointly inverting the phase‐velocity dispersions of Rayleigh waves, including short‐period (0.3–2 s) multimode dispersions using the frequency‐Bessel transform method and the long‐period (2–6 s) fundamental‐mode dispersions using the fast marching method. Our results show that the Vs inhomogeneities agree well with the distribution of geological units. We use the 1 km/s isodepth of Vs as the reference thickness of quaternary sediments. Fengbo sag (FBS) and Dachang sag (DCS), which mainly show low velocity and density, have thick sediment thicknesses (approximately 550–600 and 320–420 m, respectively). The NE high‐velocity belt in Daxing high (DXH) has a thinner sediment thickness (∼230 m). Thus, FBS and DCS have a greater risk of earthquake hazards owing to the strong amplification effects of ground motion. Additionally, Vs distribution in the FBS, DCS, DXH, and Yanshan Fold Belt are spatially related to the medium density and buried faults (Nanyuan‐Tongxian, Daxing, and Xiadian faults). We infer that the Vs structures are associated with the controlling effects of these large normal faults and inhomogeneous strata density. The discontinuity of the NE high‐velocity belt in DXH probably results from the intense tectonic activity of Nankou‐Sunhe fault.
Separating Anthropogenically‐ and Naturally‐Caused Temperature Trends: A Systematic Approach Based On Climate Memory Analysis
Authors: Yuan Naiming , Ma Chao , Franzke Christian L. E. , Niu Hourui , Dong Wenjie
Published: May 2023

Separating anthropogenic and natural contributions to global warming is crucial for understanding and predicting climate change, but it is challenging due to the complexity of the climate system. In this study, we try to address this issue by estimating the anthropogenic forcing impacts with its long‐term persistence features properly considered. In this way, a data‐driven approach is proposed to decompose the observed trends from global and regional scales into anthropogenically‐ and naturally‐caused trends. We find that there is a continuous anthropogenic global warming trend since the beginning of the last century, even during the recent global warming hiatus period. On regional scales, the anthropogenically‐forced trends among regions are found at a similar level, while their unevenly distributed warming trends among regions may be attributed to natural causes. Our findings provide new insight into climate warming, and our approach based on persistence analysis provides a new perspective for attribution studies.
Magnetotail Ion Acceleration in 3‐D Global Hybrid Simulations
Authors: Shi Feng , Guo Zhifang , Lin Yu , Wang Xueyi , Cheng Lei
Published: May 2023

Particle injection by magnetotail reconnection plays an important role in the magnetospheric physics, since these particles may be accelerated to high energy and constituent energetic particles in the inner magnetosphere. In this paper, we trace such ion particles from a near‐Earth reconnection region to the inner magnetosphere self‐consistently in a 3‐D global hybrid simulation using ANGIE3D, and demonstrate the important roles of the earthward fast flows in the ion acceleration process in the tail plasma sheet. Although the particles can gain several times of their initial energy from the reconnection X‐line region, a dramatic increase of the ion energy (from a few keV up to a few tens of keV) occurs in a very short period of time due to their encounter with the earthward fast flows. A large portion (≳70%) of the earthward moving particles around the focused reconnection site encounter fast flows and are significantly accelerated. Our results indicate that fast flow electric fields play major roles, as opposed to other common mechanisms such as the adiabatic Betatron and Fermi processes, in ion acceleration from the near‐tail region to the inner magnetosphere. In addition, dipolarization fronts associated with magnetic reconnection also affect the particle acceleration. On the global scale, it is found that particles can encounter the reconnection region first and then return to the fast flows multiple times, and thus the acceleration is through multiple local acceleration processes, leading to particle energy ∼50 keV by reconnection/fast flows in the tail. The global simulation shows that particle acceleration involves multiple regions during their injection into the ring current.
My Career—Accomplishments, Experiences, and Work‐Life Balance
Authors: LeMone Margaret A.
Published: May 2023

In describing my career, I have tried to share the experiences as well as the resume‐worthy accomplishments, trying to reflect not only the difficulties encountered but also the help from people along the way. After beginning with a short biography (initial conditions, education and training, career in a nutshell), I highlight some of the struggles and successes along the way (being a woman in the field, my supervisors, my promotions, work‐life balance, finally seminars, and retirement), before closing with something I've learned (advice) and an acknowledgment section, regretting that I almost certainly left someone out.
Genomic and transcriptomic profiling reveal molecular characteristics of parathyroid carcinoma
Authors: Jo Se-Young , Hong Namki , Lee Seunghyun , Jeong Jong Ju , Won Jeongsoo , Park Jiho , Kim Gi Jeong , Kim Sang Kyum , Kim Sangwoo , Rhee Yumie
Published: May 2023

Genomic and transcriptomic profiling has enhanced the diagnostic and treatment options for many cancers. However, the molecular characteristics of parathyroid cancer remain largely unexplored, thereby limiting the development of new therapeutic interventions. Herein, we conducted genomic and transcriptomic sequencing of 50 parathyroid tissues (12 carcinomas, 28 adenomas, and 10 normal tissues) to investigate the intrinsic and comparative molecular features of parathyroid carcinoma. We confirmed multiple two-hit mutation patterns in cell division cycle 73 (CDC73) that converged to biallelic inactivation, calling into question the presence of a second hit in other genes. In addition, allele-specific repression of CDC73 in copies with germline-truncating variants suggested selective pressure prior to tumorigenesis. Transcriptomic analysis identified upregulation of the expression of E2F targets, KRAS and TNF-alpha signaling, and epithelial-mesenchymal transition pathways in carcinomas compared to adenomas and normal tissues. A molecular classification model based on carcinoma-specific genes clearly separated carcinomas from adenomas and normal tissues, the clinical utility of which was demonstrated in two patients with uncertain malignant potential. A deeper analysis of gene expression and functional prediction suggested that Wilms tumor 1 (WT1) is a potential biomarker for CDC73-mutant parathyroid carcinoma, which was further validated through immunohistochemistry. Overall, our study revealed the genomic and transcriptomic profiles of parathyroid carcinoma and may help direct future precision diagnostic and therapeutic improvements.
DDIAS, DNA damage-induced apoptosis suppressor, is a potential therapeutic target in cancer
Authors: Im Joo-Young , Kang Mi-Jung , Kim Bo-Kyung , Won Misun
Published: May 2023

Increasing evidence indicates that DNA damage-induced apoptosis suppressor (DDIAS) is an oncogenic protein that is highly expressed in a variety of cancers, including colorectal cancer, lung cancer, breast cancer, and hepatocellular carcinoma (HCC). The discovery of DDIAS as a novel therapeutic target and its role in human cancer biology is fascinating and noteworthy. Recent studies have shown that DDIAS is involved in tumorigenesis, metastasis, DNA repair and synthesis, and drug resistance and that it plays multiple roles with distinct binding partners in several human cancers. This review focuses on the function of DDIAS and its regulatory proteins in human cancer as potential targets for cancer therapy, as well as the development and future prospects of DDIAS inhibitors.
Sex differences in the progression of glucose metabolism dysfunction in Alzheimer’s disease
Authors: Park Jong-Chan , Lim Hanbyeol , Byun Min Soo , Yi Dahyun , Byeon Gihwan , Jung Gijung , Kim Yu Kyeong , Lee Dong Young , Han Sun-Ho , Mook-Jung Inhee
Published: May 2023

Alzheimer’s disease (AD) is a common neurodegenerative disease characterized by amyloid plaques and impaired brain metabolism. Because women have a higher prevalence of AD than men, sex differences are of great interest. Using cross-sectional and longitudinal data, we showed sex-dependent metabolic dysregulations in the brains of AD patients. Cohort 1 (South Korean, n = 181) underwent Pittsburgh compound B-PET, fluorodeoxyglucose-PET, magnetic resonance imaging, and blood biomarker (plasma tau and beta-amyloid 42 and 40) measurements at baseline and two-year follow-ups. Transcriptome analysis of data from Cohorts 2 and 3 (European, n = 78; Singaporean, n = 18) revealed sex differences in AD-related alterations in brain metabolism. In women (but not in men), all imaging indicators displayed consistent correlation curves with AD progression. At the two-year follow-up, clear brain metabolic impairment was revealed only in women, and the plasma beta-amyloid 42/40 ratio was a possible biomarker for brain metabolism in women. Furthermore, our transcriptome analysis revealed sex differences in transcriptomes and metabolism in the brains of AD patients as well as a molecular network of 25 female-specific glucose metabolic genes (FGGs). We discovered four key-attractor FGG genes (ALDOA, ENO2, PRKACB, and PPP2R5D) that were associated with amyloid/tau-related genes (APP, MAPT, BACE1, and BACE2). Furthermore, these genes successfully distinguished amyloid positivity in women. Understanding sex differences in the pathogenesis of AD and considering these differences will improve development of effective diagnostics and therapeutic treatments for AD.
Suppression of local inflammation via galectin-anchored indoleamine 2,3-dioxygenase
Authors: Bracho-Sanchez Evelyn , Rocha Fernanda G. , Bedingfield Sean K. , Partain Brittany D. , Macias Sabrina L. , Brusko Maigan A. , Colazo Juan M. , Fettis Margaret M. , Farhadi Shaheen A. , Helm Eric Y. , Koenders Kevin , Kwiatkowski Alexander J. , Restuccia Antonietta , Morales Bethsymarie Soto , Wanchoo Arun , Avram Dorina , Allen Kyle D. , Duvall Craig L. , Wallet Shannon M. , Hudalla Gregory A. , Keselowsky Benjamin G.
Published: May 2023

The treatment of chronic inflammation with systemically administered anti-inflammatory treatments is associated with moderate-to-severe side effects, and the efficacy of locally administered drugs is short-lived. Here we show that inflammation can be locally suppressed by a fusion protein of the immunosuppressive enzyme indoleamine 2,3-dioxygenase 1 (IDO) and galectin-3 (Gal3). Gal3 anchors IDO to tissue, limiting the diffusion of IDO-Gal3 away from the injection site. In rodent models of endotoxin-induced inflammation, psoriasis, periodontal disease and osteoarthritis, the fusion protein remained in the inflamed tissues and joints for about 1 week after injection, and the amelioration of local inflammation, disease progression and inflammatory pain in the animals were concomitant with homoeostatic preservation of the tissues and with the absence of global immune suppression. IDO-Gal3 may serve as an immunomodulatory enzyme for the control of focal inflammation in other inflammatory conditions.
Prognostic significance of albuminuria in elderly of various ages with diabetes
Authors: Hwang Subin , Lee Kyungho , Park Jeeeun , Kim Do Hee , Jeon Junseok , Jang Hye Ryoun , Hur Kyu Yeon , Kim Jae Hyeon , Huh Wooseong , Kim Yoon-Goo , Lee Jung Eun
Published: May 2023

It remains uncertain whether albuminuria can identify elderly patients with diabetes at a high risk of incident end-stage kidney disease (ESKD) or mortality. 3065 patients (aged ≥ 65 years) with type 2 diabetes were included. We examined the association between albuminuria stages (normoalbuminuria, A1; microalbuminuria, A2; and macroalbuminuria, A3) and the risk of incident ESKD and all-cause mortality for each age group (65–69, 70–74, and ≥ 75 years). A2 and A3 were observed in 25.5% and 9.4% of the subjects, respectively. For A1, A2, and A3, the probabilities of ESKD at 8 years were 1.0%, 6.3%, and 29.7% (P < 0.001 for all), and the all-cause mortality was 13.1%, 27.4%, and 31.7% (P < 0.001 for A1 vs A2, P < 0.001 for A1 vs A3), respectively. Albuminuria stages were independently associated with an increased risk of ESKD [fully adjusted hazard ratios (HR): 3.650 (1.987–6.702) for A2, 10.404 (5.706–18.972) for A3 vs. A1]. The HRs of all-cause mortality were 1.742 (1.411–2.153) for A2 and 1.810 (1.344–2.441) for A3. The associations between albuminuria stages and the risk of ESKD and all-cause mortality were consistent across all age groups. Even microalbuminuria is also a risk factor for incident ESKD and mortality in elderly patients with diabetes.
Cholesterol biosynthesis modulates differentiation in murine cranial neural crest cells
Authors: Pascual Florencia , Icyuz Mert , Karmaus Peer , Brooks Ashley , Van Gorder Elizabeth , Fessler Michael B. , Shaw Natalie D.
Published: May 2023

Cranial neural crest cells (cNCC) are a multipotent embryonic cell population that give rise to a diverse set of cell types. These cells are particularly vulnerable to external metabolic stressors, as exemplified by the association between maternal hyperglycemia and congenital malformations. We were interested in studying the effect of various concentrations of glucose and pyruvate on cNCC metabolism, migration, and differentiation using an established murine neural crest cell model (O9-1). We unexpectedly observed a pattern of gene expression suggestive of cholesterol biosynthesis induction under glucose depletion conditions in O9-1 cells. We further showed that treatment with two different cholesterol synthesis inhibitors interfered with cell migration and differentiation, inhibiting chondrogenesis while enhancing smooth muscle cell differentiation. As congenital arhinia (absent external nose), a malformation caused by mutations in SMCHD1, appears to represent, in part, a defect in cNCC, we were also interested in investigating the effects of glucose and cholesterol availability on Smchd1 expression in O9-1 cells. Smchd1 expression was induced under high glucose conditions whereas cholesterol synthesis inhibitors decreased Smchd1 expression during chondrogenesis. These data highlight a novel role for cholesterol biosynthesis in cNCC physiology and demonstrate that human phenotypic variability in SMCHD1 mutation carriers may be related, in part, to SMCHD1’s sensitivity to glucose or cholesterol dosage during development.
Respiratory entrainment of the locus coeruleus modulates arousal level to avoid physical risks from external vibration
Authors: Iwamoto Masami , Yonekura Shogo , Atsumi Noritoshi , Hirabayashi Satoko , Kanazawa Hoshinori , Kuniyoshi Yasuo
Published: May 2023

Slow rocking chairs can easily put people to sleep, while violent shaking, such as during earthquakes, may lead to rapid awakening. However, the influence of external body vibrations on arousal remains unclear. Herein, a computational model of a locus coeruleus (LC)-norepinephrine (NE) system and cardio-respiratory system were used to show that respiratory entrainment of the LC modulates arousal levels, which is an adaptation to avoid physical risks from external vibration. External vibrations of sinusoidal waves with different frequencies ranging from 0.1 to 20 [Hz] were applied to the LC based on the results of previous studies. We found that respiratory entrainment of the LC decreased the breathing rate (BR) and heart rate (HR) to maintain the HR within its normal range. Furthermore, 1:1 phase locking enhanced arousal level while phase-amplitude coupling decreased it for larger vibration stimuli. These findings suggest that respiratory entrainment of the LC might automatically modulate cardio-respiratory system homeostasis and arousal levels for performance readiness (fight/flight or freeze) to avoid physical risks from larger external vibrations.
Reproductives signature revealed by protein profiling and behavioral bioassays in termite
Authors: Ruhland Fanny , Gabant Guillaume , Toussaint Timothée , Nemcic Matej , Cadène Martine , Lucas Christophe
Published: May 2023

Proteins are known to be social interaction signals in many species in the animal kingdom. Common mediators in mammals and aquatic species, they have seldom been identified as such in insects' behaviors. Yet, they could represent an important component to support social signals in social insects, as the numerous physical contacts between individuals would tend to favor the use of contact compounds in their interactions. However, their role in social interactions is largely unexplored: are they rare or simply underestimated? In this preliminary study, we show that, in the termite Reticulitermes flavipes, polar extracts from reproductives trigger body-shaking of workers (a vibratory behavior involved in reproductives recognition) while extracts from workers do not. Molecular profiling of these cuticular extracts using MALDI-TOF mass spectrometry reveals higher protein diversity in reproductives than in workers and a sex-specific composition exclusive to reproductives. While the effects observed with extracts are not as strong as with live termites, these results open up the intriguing possibility that social signaling may not be limited to cuticular hydrocarbons or other non-polar, volatile chemicals as classically accepted. Our results suggest that polar compounds, in particular some of the Cuticular Protein Compounds (CPCs) shown here by MALDI to be specific to reproductives, could play a significant role in insect societies. While this study is preliminary and further comprehensive molecular characterization is needed to correlate the body-shaking triggering effects with a given set of polar compounds, this exploratory study opens new perspectives for understanding the role of polar compounds such as proteins in caste discrimination, fertility signaling, or interspecific insect communication.
Unsatisfactory reproducibility of interstitial inflammation scoring in allograft kidney biopsy
Authors: Huang Shun-Chen , Lin Yi-Jia , Wen Mei-Chin , Lin Wei-Chou , Fang Pei-Wei , Liang Peir-In , Chuang Hao-Wen , Chien Hui-Ping , Chen Tai-Di
Published: May 2023

Interstitial inflammation scoring is incorporated into the Banff Classification of Renal Allograft Pathology and is essential for the diagnosis of T-cell mediated rejection. However, its reproducibility, including inter-rater and intra-rater reliabilities, has not been carefully investigated. In this study, eight renal pathologists from different hospitals independently scored 45 kidney allograft biopsies with varying extents of interstitial inflammation. Inter-rater reliabilities and intra-rater reliabilities were investigated by kappa statistics and conditional agreement probabilities. Individual pathologists’ scoring patterns were examined by chi-squared tests and proportions tests. The mean pairwise kappa values for inter-rater reliability were 0.27, 0.30, and 0.26 for the Banff i score, ti score, and i-IFTA, respectively. No rater pair performed consistently better or worse than others on all three scorings. After dichotomizing the scores into two groups (none/mild and moderate/severe inflammation), the averaged conditional agreements ranged from 47.1% to 50.0%. The distributions of the scores differed, but some pathologists persistently scored higher or lower than others. Given the important role of interstitial inflammation scoring in the diagnosis of T-cell mediated rejection, transplant practitioners should be aware of the possible clinical implications of the far-from-optimal reproducibility.
The role of water mass advection in staging of the Southern Ocean Salpa thompsoni populations
Authors: Henschke Natasha , Espinasse Boris , Stock Charles A. , Liu Xiao , Barrier Nicolas , Pakhomov Evgeny A.
Published: May 2023

Salpa thompsoniis an important grazer in the Southern Ocean. Their abundance in the western Antarctic Peninsula is highly variable, varying by up to 5000-fold inter-annually. Here, we use a particle-tracking model to simulate the potential dispersal of salp populations from a source location in the Antarctic Circumpolar Current (ACC) to the Palmer Long Term Ecological Research (PAL LTER) study area. Tracking simulations are run from 1998 to 2015, and compared against both a stationary salp population model simulated at the PAL LTER study area and observations from the PAL LTER program. The tracking simulation was able to recreate closely the long-term trend and the higher abundances at the slope stations. The higher abundances observed at slope stations are likely due to the advection of salp populations from a source location in the ACC, highlighting the significant role of water mass circulation in the distribution and abundance of Southern Ocean salp populations.
High side-mode suppression ratio with a Vernier effect single-mode laser using triple coupled microrings
Authors: Jahangiri Milad , Moradiani Fatemeh , Parsanasab Gholam-Mohammad , Mirmohammadi Mohsen
Published: May 2023

The development of single-mode lasers with a high side-mode suppression ratio (SMSR) is challenging but highly desirable for integrated photonics devices and long-distance communications due to their high spectral purity and stability. Here, we demonstrate a single-mode laser with a high side-mode suppression ratio based on size-mismatched triple-coupled microrings. With the exact engineering of several key parameters of the structure like air gap and radii of microrings for controlling the free spectral range (FSR), a predominant mode is selected to lase in amplified spontaneous emission (ASE) of the gain material and all side and high order modes are suppressed by Vernier effect. In this work, we show that a single-mode operation is efficiently generated with an improved side-mode suppression ratio of over 20 dB in a three-ring-coupled microcavity laser. The single-frequency output persists for a wide power range. The theoretical calculations and numerical simulations’ results confirm the validity of the experimental results. Our structural engineering creates new opportunities in a variety of frontier applications in single-mode lasers and high-quality sensors.