bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthesis of Carborane–Thiazole Conjugates as Tyrosinase and 11β-Hydroxysteroid Dehydrogenase Inhibitors: Antiproliferative Activity and Molecular Docking Studies
Authors: Donarska Beata , Cytarska Joanna , Kołodziej-Sobczak Dominika , Studzińska Renata , Kupczyk Daria , Baranowska-Łączkowska Angelika , Jaroch Karol , Szeliska Paulina , Bojko Barbara , Różycka Daria , Olejniczak Agnieszka B. , Płaziński Wojciech , Łączkowski Krzysztof Z.
Published: October 2024

The presented study depicts the synthesis of 11 carborane–thiazole conjugates with anticancer activity, as well as an evaluation of their biological activity as inhibitors of two enzymes: tyrosinase and 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1). The overexpression of tyrosinase results in the intracellular accumulation of melanin and can be observed in melanoma. The overexpression of 11β-HSD1 results in an elevation of glucocorticoid levels and has been associated with the aggravation of metabolic disorders such as type II diabetes mellitus and obesity. Recently, as the comorbidity of melanomas and metabolic disorders is being recognized as an important issue, the search for new therapeutic options has intensified. This study demonstrates that carborane–thiazole derivatives inhibit both enzymes, exerting beneficial effects. The antiproliferative action of all newly synthesized compounds was evaluated using three cancer cell lines, namely A172 (human brain glioblastoma), B16F10 (murine melanoma) and MDA-MB-231 (human breast adenocarcinoma), as well as a healthy control cell line of HUVEC (human umbilical vein endothelial cells). The results show that 9 out of 11 newly synthesized compounds demonstrated similar antiproliferative action against the B16F10 cell line to the reference drug, and three of these compounds surpassed it. To the best of our knowledge, this study is the first to demonstrate dual inhibitory action of carborane–thiazole derivatives against both tyrosinase and 11β-HSD1. Therefore, it represents the first step towards the simultaneous treatment of melanoma and comorbid diseases such as type II diabetes mellitus.
Insight into the Reversible Hydrogen Storage of Titanium-Decorated Boron-Doped C20 Fullerene: A Theoretical Prediction
Authors: Chai Zhiliang , Liu Lili , Liang Congcong , Liu Yan , Wang Qiang
Published: October 2024

Hydrogen storage has been a bottleneck factor for the application of hydrogen energy. Hydrogen storage capacity for titanium-decorated boron-doped C20 fullerenes has been investigated using the density functional theory. Different boron-doped C20 fullerene absorbents are examined to avoid titanium atom clustering. According to our research, with three carbon atoms in the pentagonal ring replaced by boron atoms, the binding interaction between the Ti atom and C20 fullerene is stronger than the cohesive energy of titanium. The calculated results revealed that one Ti atom can reversibly adsorb four H2 molecules with an average adsorption energy of −1.52 eV and an average desorption temperature of 522.5 K. The stability of the best absorbent structure with a gravimetric density of 4.68 wt% has been confirmed by ab initio molecular dynamics simulations. These findings suggest that titanium-decorated boron-doped C20 fullerenes could be considered as a potential candidate for hydrogen storage devices.
Structure–Function Relationships and Health-Promoting Properties of the Main Nutraceuticals of the Cactus Pear (Opuntia spp.) Cladodes: A Review
Authors: Koufan Meriyem , Choukrane Basma , Mazri Mouaad Amine
Published: October 2024

Over the past decade, several studies have established a direct link between functional foods, nutraceuticals, and a reduced risk of oxidative-stress-related diseases. Nutraceuticals, which encompass a variety of bioactive molecules, exhibit both nutritional and therapeutic properties. The cactus pear (Opuntia spp.) is a plant genus with many species recognized as functional foods, largely attributed to their high content of nutraceuticals, including polyphenols, fatty acids, vitamins, amino acids, pigments, and phytosterols. These compounds of different structures and functions possess different biological activities, contributing to the health-promoting properties of cactus pear. This makes cactus pears a valuable plant for the food, cosmetic, and pharmaceutical industries. While extensive research has focused on the nutritional profile of cactus pear fruits, the cladodes have received comparatively limited attention. Notably, the nutritional composition of cladodes can exhibit considerable variability, influenced by species and growing conditions. Furthermore, although various bioactive compounds have been identified in cladodes, studies elucidating their mechanisms of action, health benefits, and potential therapeutic applications remain insufficient. Addressing these gaps is crucial for enhancing the understanding and utilization of cactus pear cladodes. This paper provides a comprehensive overview of the structure–function relationships of the main nutraceuticals found in cactus pear cladodes. It synthesizes data from recent and relevant literature to elucidate the content of these compounds in relation to species and geographical origin, while also detailing the main biological activities and health-promoting benefits associated with cactus pear cladodes.
Enhanced Immobilization of Enzymes on Plasma Micro-Nanotextured Surfaces and Microfluidics: Application to HRP
Authors: Vorvi Stefania , Tsougeni Katerina , Tserepi Angeliki , Kakabakos Sotirios , Petrou Panagiota , Gogolides Evangelos
Published: October 2024

The enhanced and direct immobilization of the enzyme horseradish peroxidase on poly(methyl methacrylate) (PMMA) microchannel surfaces to create a miniaturized enzymatic reactor for the biocatalytic oxidation of phenols is demonstrated. Enzyme immobilization occurs by physical adsorption after oxygen plasma treatment, which micro-nanotextures the PMMA surfaces. A five-fold enhancement in immobilized enzyme activity was observed, attributed to the increased surface area and, therefore, to a higher quantity of immobilized enzymes compared to an untreated PMMA surface. The enzymatic reaction yield reached 75% using a flow rate of 2.0 μL/min for the reaction mixture. Additionally, the developed microreactor was reused more than 16 times without affecting the enzymatic conversion yield. These results demonstrate the potential of microchannels with plasma micro/nanotextured surfaces for the rapid and facile fabrication of microfluidic enzymatic microreactors with enhanced catalytic activity and stability.
Enhancing the Photocatalytic Performance for Norfloxacin Degradation by Fabricating S-Scheme ZnO/BiOCl Heterojunction
Authors: Yang Rongpeng , Lu Guang , Liang Hongyu , Li Zheng , Liang Jiling , Chen Zhen
Published: October 2024

Construction of S-scheme heterojunctions can effectively limit the recombination of photogenerated e− and h+, thus improving photocatalytic activity. Therefore, S-scheme ZnO/BiOCl (molar ratio = 1:2) n–n heterojunctions were synthesized via a hydrothermal–hydrolysis combined method in this study. The physical and chemical properties of the ZnO/BiOCl heterojunctions were characterized by XRD, XPS, SEM, TEM, DRS, N2 adsorption–desorption and ESR. Additionally, the photoelectric performances of ZnO/BiOCl heterojunctions were investigated with TPR, M–S plot and EIS. The results show that photocatalytic degradation of NOR by ZnO/BiOCl reached to 94.4% under simulated sunlight, which was 3.7 and 1.6 times greater than that of ZnO and BiOCl, respectively. The enhanced photodegradation ability was attributed to the enhancement of the internal electric field between ZnO and BiOCl, facilitating the active separation of photogenerated electrons and holes. The radical capture experiments and ESR results illustrate that the contribution of reactive species was in descending order of ·OH > h+ > ·O2− and a possible mechanism for the photodegradation of NOR over S-scheme ZnO/BiOCl heterojunctions was proposed.
Biosynthesis and Characterization of Iron Oxide Nanoparticles Using Chenopodium quinoa Extract
Authors: Marcos-Carrillo Mercedes del Pilar , Checca-Huaman Noemi-Raquel , Passamani Edson C. , Ramos-Guivar Juan A.
Published: October 2024

In this study, we achieved the biosynthesis of novel 7–8 nm iron-oxide nanoparticles in the presence of different concentrations (5 to 50% w/v) of commercial white quinoa extract. Initially, quinoa extract was prepared at various concentrations by a purification route. The biosynthesis optimization was systematically monitored by X-ray diffraction, and the Rietveld quantitative analysis showed the presence of goethite (5 to 10 wt.%) and maghemite phases. The first phase disappeared upon increasing the organic loading (40 and 50% w/v). The organic loading was corroborated by thermogravimetric measurements, and it increased with quinoa extract concentration. Its use reduces the amount of precipitation agent at high quinoa extract concentrations with the formation of magnetic nanoparticles with hard ferrimagnetic character (42 and 11 emu g−1). The enrichment of hydroxyl groups and the negative zeta potential above pH = 7 were corroborated by a reduction in the point of zero charge in all the samples. For alkaline values, the zeta potential values were above the stability range, indicating highly stable chemical species. The evidence of hydroxyl and amide functionalization was qualitatively observed using infrared analysis, which showed that the carboxyl (quercetin/kaempferol), amide I, and amide III chemical groups are retained after biosynthesis. The resultant biosynthesized samples can find applications in environmental remediation due to the affinity of the chemical agents present on the particle surfaces and easy-to-handle them magnetically.
The Influence of Thickness and Spectral Properties of Green Color-Emitting Polymer Thin Films on Their Implementation in Wearable PLED Applications
Authors: Papadopoulos Kyparisis , Tselekidou Despoina , Zachariadis Alexandros , Laskarakis Argiris , Logothetidis Stergios , Gioti Maria
Published: October 2024

A systematic investigation of optical, electrochemical, photophysical, and electrooptical properties of printable green color-emitting polymer (poly(9,9-dioctylfluorene-alt-bithiophene)) (F8T2) and spiro-copolymer (SPG-01T) was conducted to explore their potentiality as an emissive layer for wearable polymer light-emitting diode (PLED) applications. We compared the two photoactive polymers in terms of their spectral characteristics and color purity, as these are the most critical factors for wearable lighting sources and optical sensors. Low-cost, solution-based methods and facile architecture were applied to produce rigid and flexible light-emitting devices with high luminance efficiencies. Emission bandwidths, color coordinates, operational characteristics, and luminance were also derived to evaluate the device’s stability. The tuning of emission’s spectral features by layer thickness variation was realized and was correlated with the interplay between H-aggregates and J-aggregates formations for both conjugated polymers. Finally, we applied the functional green light-emitting PLED devices based on the two studied materials for the detection of Rhodamine 6G. It was determined that the optical detection of the R6G photoluminescence is heavily influenced by the emission spectrum characteristics of the PLED and changes in the thickness of the active layer.
Removal of Nitrogen, Phosphates, and Chemical Oxygen Demand from Community Wastewater by Using Treatment Wetlands Planted with Ornamental Plants in Different Mineral Filter Media
Authors: Marín-Muñiz José Luis , Ortega-Pineda Gonzalo , Zitácuaro-Contreras Irma , Vidal-Álvarez Monserrat , Martínez-Aguilar Karina E. , Álvarez-Hernández Luis M. , Zamora-Castro Sergio
Published: October 2024

This study aimed to explore the impact of various ornamental plants (Heliconia psittacorum, Etlingera elatior, Spatyphilum walisii) grown in different filter media (porous river rock (PR) and tepezyl (TZ)) on the removal of pollutants in vertical-subsurface-microcosm treatment wetlands (TWs). This study also sought to assess the adaptability of these plant species to TW conditions. Twenty-four microcosm systems were utilized, with twelve containing PR and twelve containing TZ as the filter media. Each porous media type had three units planted with each species, and three were left unplanted. Rural community wastewater was treated in the TWs. The results showed no significant differences in the effects of the porous media on pollutant removal performance (p > 0.05). However, it was noted that while both porous media were efficient, TZ, a residue of construction materials, is recommended for sites facing economic constraints. Additionally, the removal efficiency was found to be independent of the type of ornamental plant used (p > 0.05); however, the measured parameters varied with plant spp. The adaptation of the plants varied depending on the species. H. psittacorum grew faster and produced a larger number of flowers compared to the other species (20–22 cm). S. wallisii typically produced 7–8 flowers. E. elatior did not produce flowers, and some plants showed signs of slight disease and pests, with the leaves turning yellow. In terms of plant biomass, the type of porous media used did not have a significant effect on the production of above (p = 0.111) or below-ground biomass (p = 0.092). The removal percentages for COD in the presence and absence of plants were in the ranges of 64–77% and 27–27.7%, respectively. For TN, the numbers were 52–65% and 30–31.8%, and for N-NO3, they were 54–60% and 12–18%. N-NH4 saw removal rates of 67–71% and 28–33%, while P-PO4 saw removal rates of 60–72% and 22–25%. The difference in removal percentages between microcosms with and without plants ranged from 30 to 50%, underscoring the importance of plants in the bio-removal processes (phytoremediation). These results reveal that incorporating ornamental plants in TWs with TZ for wastewater in rural areas holds great promise for enhancing the visual appeal of these systems and ultimately gaining public approval. Our findings also enable us to offer recommendations for selecting suitable plants and substrates, as well as designing combinations for TWs.
The Relationship between Plant-Based Diet Indices and Sleep Health in Older Adults: The Mediating Role of Depressive Symptoms and Anxiety
Authors: Liu Junping , Liu Zhaoyue , Zhou Yue , Wu Lin , Wang Nan , Liu Xinru , Liu Yaping , Yin Xinle , Yang Aiying , Liang Libo
Published: October 2024

Background: The goal of our research was to determine the effects of plant-based dietary patterns on sleep health among older adults and to examine the parallel mediated effects of anxiety and depression. Methods: This investigation utilized data obtained from the 2018 Chinese Longitudinal Healthy Longevity Survey (CLHLS) and contained 6853 participants. Logistic regression and the restricted cubic splines (RCSs) model were employed to examine how plant-based dietary patterns affect sleep health. Additionally, Amos 26.0 was used to construct a structural equation model to examine the parallel mediated effects of anxiety and depression. Results: A higher plant-based diet index (PDI) was connected to higher odds of better sleep quality (OR = 1.209, 95% CI: 1.039–1.407) and sleep duration (OR = 1.241, 95% CI: 1.072–1.437). Conversely, an elevated unhealthy plant-based diet index (uPDI) was correlated with a lower likelihood of sleep quality (OR = 0.678, 95% CI: 0.574–0.800) and sleep duration (OR = 0.762, 95% CI: 0.647–0.896). The RCSs regression further identified a significant dose–response relationship. Mediation analysis confirmed that anxiety and depression partially mediate the relationship between plant-based diets and sleep health. Conclusions: Our study exhibited significant correlations between plant-based diets and sleep health in the elderly. Depression and anxiety were determined as parallel mediators between plant-based diets and sleep health. Controlling early dietary patterns and affective disorder could help improve sleep quality in older adults.
Effects of Human Milk Oligosaccharide 2′-Fucosyllactose Ingestion on Weight Loss and Markers of Health
Authors: Ko Joungbo , Yoo Choongsung , Xing Dante , Chun Jisun , Gonzalez Drew E. , Dickerson Broderick L. , Leonard Megan , Jenkins Victoria , van der Merwe Marie , Slupsky Carolyn M. , Sowinski Ryan , Rasmussen Christopher J. , Kreider Richard B.
Published: October 2024

Background: 2′-Fucosyllactose (2′-FL) is an oligosaccharide contained in human milk and possesses prebiotic and anti-inflammatory effects, which may alleviate skeletal muscle atrophy under caloric restriction. This study evaluated the impacts of 12 weeks of 2′-FL supplementation in conjunction with exercise (10,000 steps/day, 5 days/week) and energy-reduced (−300 kcals/day) dietary interventions on changes in body composition and health-related biomarkers. Methods: A total of 41 overweight and sedentary female and male participants (38.0 ± 13 years, 90.1 ± 15 kg, 31.6 ± 6.6 kg/m2, 36.9 ± 7% fat) took part in a randomized, double-blind, and placebo-controlled study. The participants underwent baseline assessments and were then assigned to ingest 3 g/day of a placebo (PLA) or Momstamin 2′-F while initiating the exercise and weight-loss program. Follow-up tests were performed after 6 and 12 weeks. Data were analyzed using general linear model statistics with repeated measures and mean changes from baseline values with 95% confidence intervals (CIs). Results: No group × time × sex interaction effects were observed, so group × time effects are reported. Participants in both groups saw comparable reductions in weight. However, those with 2′-FL demonstrated a significantly greater reduction in the percentage of body fat and less loss of the fat-free mass. Additionally, there was evidence that 2′-FL supplementation promoted more favorable changes in resting fat oxidation, peak aerobic capacity, IL-4, and platelet aggregation, with some minimal effects on the fermentation of short-chain fatty acids and monosaccharides in fecal samples. Moreover, participants’ perceptions regarding some aspects of the functional capacity and ratings of the quality of life were improved, and the supplementation protocol was well tolerated, although a small, but significant, decrease in BMC was observed. Conclusions: The results support contentions that dietary supplementation of 2′-FL (3 g/d) can promote fat loss and improve exercise- and diet-related markers of health and fitness in overweight sedentary individuals initiating an exercise and weight-loss program. Further research is needed to explore the potential health benefits of 2′-FL supplementation in both healthy and elderly individuals (Registered clinical trial #NCT06547801).
Are Cooked Nutritious School Lunches Associated with Improved Attendance? Findings from the 2022–2023 Tasmanian School Lunch Project
Authors: Smith Kylie J. , Cleland Verity , Chappell Kate , Fraser Brooklyn , Sutton Laura , Proudfoot Fiona , Dunbabin Julie , Jose Kim
Published: October 2024

Background/Objectives: During 2022–2023, the School Lunch Project (SLP) provided free nutritious cooked lunches 1–4 days per week to Kinder to Grade 10 students attending 30 schools in areas of high disadvantage in Tasmania, Australia. This analysis examined if the SLP was associated with student attendance. Methods: Staff (teachers, support staff, and principals) from 12 schools completed an online survey and/or participated in focus groups/interviews. Government-held, objectively measured, grade-level attendance data were provided for 17 SLP and 11 matched comparison schools for 2018–2023. Linear mixed models compared attendance on school lunch and non-school lunch days in SLP schools. Difference-in-difference regression compared attendance between SLP and comparison schools. Qualitative data were analysed thematically. Results: Sixty-five staff completed surveys, where 22% reported that increased attendance was a benefit of the SLP. Similar findings were observed in the staff focus groups/interviews (N = 51). Mean attendance was similar on school lunch and non-school lunch days among the SLP schools during 2022 (difference: 0.04, 95% CI: −0.5, 0.6) and 2023 (difference 0.1, 95% CI: −0.2, 0.4) and similar between SLP and comparison schools (average treatment effect in the treated: 1.2, 95% CI: −0.7, 3.0). Conclusions: The SLP was perceived by some staff to improve attendance but was not associated with objectively measured attendance examined at the grade level.
Dietary Fibre Intake, Adiposity, and Metabolic Disease Risk in Pacific and New Zealand European Women
Authors: Renall Nikki , Merz Benedikt , Douwes Jeroen , Corbin Marine , Slater Joanne , Tannock Gerald W. , Firestone Ridvan , Kruger Rozanne , Te Morenga Lisa
Published: October 2024

Background/Objectives: To assess associations between dietary fibre intake, adiposity, and odds of metabolic syndrome in Pacific and New Zealand European women. Methods: Pacific (n = 126) and New Zealand European (NZ European; n = 161) women (18–45 years) were recruited based on normal (18–24.9 kg/m2) and obese (≥30 kg/m2) BMIs. Body fat percentage (BF%), measured using whole body DXA, was subsequently used to stratify participants into low (<35%) or high (≥35%) BF% groups. Habitual dietary intake was calculated using the National Cancer Institute (NCI) method, involving a five-day food record and semi-quantitative food frequency questionnaire. Fasting blood was analysed for glucose and lipid profile. Metabolic syndrome was assessed with a harmonized definition. Results: NZ European women in both the low- and high-BF% groups were older, less socioeconomically deprived, and consumed more dietary fibre (low-BF%: median 23.7 g/day [25–75-percentile, 20.1, 29.9]; high-BF%: 20.9 [19.4, 24.9]) than Pacific women (18.8 [15.6, 22.1]; and 17.8 [15.0, 20.8]; both p < 0.001). The main source of fibre was discretionary fast foods for Pacific women and whole grain breads and cereals for NZ European women. A regression analysis controlling for age, socioeconomic deprivation, ethnicity, energy intake, protein, fat, and total carbohydrate intake showed an inverse association between higher fibre intake and BF% (β= −0.47, 95% CI = −0.62, −0.31, p < 0.001), and odds of metabolic syndrome (OR = 0.91, 95% CI = 0.84, 0.98, p = 0.010) among both Pacific and NZ European women (results shown for both groups combined). Conclusions: Low dietary fibre intake was associated with increased metabolic disease risk. Pacific women had lower fibre intakes than NZ European women.
A Comparison among Score Systems for Discharging Patients from Recovery Rooms: A Narrative Review
Authors: El Aoufy Khadija , Forciniti Carolina , Longobucco Yari , Lucchini Alberto , Mangli Ilaria , Magi Camilla Elena , Bulleri Enrico , Fusi Cristian , Iovino Paolo , Iozzo Pasquale , Rizzato Nicoletta , Rasero Laura , Bambi Stefano
Published: October 2024

Introduction: The recovery room (RR) is a hospital area where patients are monitored in the early postoperative period before being transferred to the surgical ward or other specialized units. The utilization of scores in the RR context facilitates the assignment of patients to the appropriate ward and directs necessary monitoring. Some scoring systems allow nurses to select patients who can be discharged directly to their homes. Aim and methods: The aim of this narrative review was to describe and compare the scoring systems employed to discharge postoperative patients from RR, with a focus on item characteristics. Results: Nine scoring systems were identified and discussed: the “Aldrete Score System” and its modified version, the “Respiration, Energy, Alertness, Circulation, Temperature Score”, the “Post Anesthetic Discharge Scoring System”, the “White and Song Score”, the “Readiness for Discharge Assessment Tool”, the “Anesthesia and Perioperative Medicine Service Checklist”, the “Post-Anesthetic Care Tool”, the “Post-operative Quality Recovery Scale”, and the “Discerning Post Anesthesia Readiness for Transition” instrument. Discussion and conclusions: To obtain a comprehensive overview, the items included in the scoring systems were compared. Despite the availability of guidelines for patients’ discharge readiness from the RR, there is no universally recommended scoring system. Next-generation scores must be improved to ease their use, minimize errors, and increase safety. The main goals of the scores included in this narrative review were to be simple to use, feasible, intuitive, comprehensive, and flexible. However, these goals frequently conflict because patient assessment takes time, and a smart and comprehensive score may not consider some clinical parameters that may be crucial for the discharge decision. Therefore, further research should be conducted on this topic.
Measuring Instruments for Media Health Literacy: A Systematic Review of Psychometric Properties
Authors: Navas-Echazarreta Noelia , Juárez-Vela Raúl , Martínez-Sabater Antonio , Echániz-Serrano Emmanuel , Fernández-Rodrigo María Teresa , Navarro-Martínez Olga , Sancho-Sánchez Consuelo , Cobos-Rincón Ana , Rodríguez-Calvo Antonio , González-Fernández Silvia , Chover-Sierra Elena , Satústegui-Dordá Pedro José
Published: October 2024

Background: Informational overload hinders the recognition of quality information and influences a population’s health-related decisions. In this context, media health literacy aims to promote citizens’ critical analysis skills, contributing to informed decision-making. This study aims to identify the instruments used to measure the level of media health literacy and their psychometric properties. Methods: A systematic review of the scientific literature was performed in 2023. The articles were extracted from the electronic databases “Pubmed”, “Web of Science”, “Dialnet”, and “Scopus”. The search languages were limited to English, Spanish, and Portuguese. Results: Twelve articles were selected for further analysis. The described measurement instruments included five original scales and seven cross-cultural adaptations of three of them. Four scales (the Sugar-Sweetened Beverages Media Literacy scale adapted to Turkish and Chinese, along with the Media Health Literacy (MeHLit) scale and its adaptation to the Chinese language) exhibited high quality in the assessment of psychometric properties. Conclusions: These instruments allow for the measurement of an individual’s level of skill when consuming specific health information, enabling an analysis to understand the risk they are exposed to. Further research is recommended to strengthen the existing evidence and apply these tools to broader and more diverse populations.
In Vitro Evaluation of Pharmacokinetic Properties of Selected Dual COX-2 and 5-LOX Inhibitors
Authors: Bošković Jelena , Dobričić Vladimir , Savić Jelena , Rupar Jelena , Aleksić Mara , Marković Bojan , Čudina Olivera
Published: October 2024

Evaluation of pharmacokinetic properties is a significant step at the early stages of drug development. In this study, an in vitro evaluation of the pharmacokinetic properties of five newly synthesized compounds was performed. These compounds belong to N-hydroxyurea and hydroxamic acid derivatives and analogs of NSAIDs indomethacin, flurbiprofen, diclofenac, ibuprofen, and naproxen (compounds 1, 2, 3, 11, and 12, respectively) with dual COX-2 and 5-LOX inhibitory activity. Two in vitro methods (biopartitioning micellar chromatography (BMC) and PAMPA) were used to evaluate passive gastrointestinal absorption, while high-performance affinity chromatography (HPAC) and differential pulse voltammetry (DPV) were used to evaluate binding to human serum albumin (HSA). The introduction of N-hydroxyurea and hydroxamic acid groups into the structure of NSAIDs decreases both expected passive gastrointestinal absorption (BMC k values were from 3.02 to 9.50, while for NSAIDs were from 5.29 to 13.36; PAMPA –logPe values were between 3.81 and 4.76, while for NSAIDs were ≤3.46) and HSA binding (HPAC logk values were from 2.03 to 9.54, while for NSAIDs were ≥11.03; DPV peak potential shifts were between 7 and 34, while for NSAIDs were ≥54). Structural modifications of all tested compounds that increase lipophilicity could be considered to enhance their passive gastrointestinal absorption. Considering lower expected HSA binding and higher lipophilicity of tested compounds compared to corresponding NSAIDs, it can be expected that the volume of distribution of compounds 1, 2, 3, 11, and 12 will be higher. Reduced HSA binding may also decrease interactions with other drugs in comparison to corresponding NSAIDs. All tested compounds showed significant microsomal instability (25.07–58.44% decrease in concentration) in comparison to indomethacin (14.47%) and diclofenac (20.99%).
The Interplay of Exogenous Cannabinoid Use on Anandamide and 2-Arachidonoylglycerol in Anxiety: Results from a Quasi-Experimental Ad Libitum Study
Authors: Martin-Willett Renée , Skrzynski Carillon J. , Taylor Ethan M. , Sempio Cristina , Klawitter Jost , Bidwell L. Cinnamon
Published: October 2024

The public is increasingly reporting using cannabis for anxiety relief. Both cannabis use and the endocannabinoid system have been connected with anxiety relief/anxiolytic properties, but these relationships are complex, and the underlying mechanisms for them are unclear. Background/Objectives: Work is needed to understand how the endocannabinoid system, including the endocannabinoids anandamide (AEA) and 2-arachidonoylglycerol (2-AG), may be impacted by the main constituents of cannabis, Δ9-tetrahydrocannabinol (THC), and cannabidiol (CBD). Methods: The current study examined how the ab libitum use of products differing in THC and CBD affected AEA and 2-AG among 292 individuals randomly assigned to THC-dominant use (N = 92), CBD-dominant use (N = 97), THC + CBD use (N = 74), or non-use (N = 29). Results: The findings suggest that AEA levels do not change differently based on 4 weeks of cannabis use or by cannabinoid content, as AEA similarly increased across all conditions from study weeks 2 to 4. In contrast, AEA decreased at an acute administration session with product conditions containing any THC having greater AEA levels on average than the non-use condition. With regard to 2-AG, its levels appeared to primarily be affected by THC-dominant use, both acutely and over 4 weeks, when controlling for baseline cannabis use and examining study product use frequency among use conditions. Conclusions: Overall, the results continue to shed light on the complicated relationship between cannabinoid content and endocannabinoid production, and highlight the need for continued research on their interplay in human subjects.
Inhibitory Effects of Decursin Derivative against Lipopolysaccharide-Induced Inflammation
Authors: Lee Jinhee , Heo Jong-Beom , Cho Sanghee , Ryu Chang-Woo , Heo Hae-Joon , Yun Mi-Young , Nam Gaewon , Song Gyu-Yong , Bae Jong-Sup
Published: October 2024

Background: This study aims to explore the protective role of JB-V-60—a novel synthetic derivative of decur-sin—against lipopolysaccharide (LPS)-induced inflammation. Methods: We examined the effects of JB-V-60 on heme oxygenase (HO)-1, cyclooxygenase (COX)-2, and inducible nitric oxide synthase (iNOS) in LPS-activated human pulmonary artery endothelial cells (HPAECs). Additionally, we assessed its effects on iNOS, tumor necrosis factor (TNF)-α, and interleukin (IL)-1β in LPS-exposed mice. Results: JB-V-60 enhanced HO-1 levels, inhibited NF-κB activation, reduced COX-2/PGE2 and iNOS/NO concentra-tions, and lowered phosphorylation of signal transducer and activator of transcription 1. It also promoted the translocation of Nrf2 into the nucleus, allowing its binding to antioxidant response elements and resulting in reduced IL-1β in LPS-stimulated HPAECs. The reduction in iNOS/NO levels by JB-V-60 was reversed when HO-1 was inhibited via RNAi. In the animal model, JB-V-60 sig-nificantly decreased iNOS expression in lung tissues and TNF-α levels in bronchoalveolar lavage fluid. Conclusions: These findings highlight the anti-inflammatory effects of JB-V-60 and its potential as a treat-ment for inflammatory disorders.
Cell Therapy for Retinal Degenerative Diseases: Progress and Prospects
Authors: Wu Kevin Y. , Dhaliwal Jaskarn K. , Sasitharan Akash , Kalevar Ananda
Published: October 2024

Background/Objectives: Age-related macular degeneration (AMD) and retinitis pigmentosa (RP) are leading causes of vision loss, with AMD affecting older populations and RP being a rarer, genetically inherited condition. Both diseases result in progressive retinal degeneration, for which current treatments remain inadequate in advanced stages. This review aims to provide an overview of the retina’s anatomy and physiology, elucidate the pathophysiology of AMD and RP, and evaluate emerging cell-based therapies for these conditions. Methods: A comprehensive review of the literature was conducted, focusing on cell therapy approaches, including embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), mesenchymal stem cells (MSCs), and retinal progenitor cells. Preclinical and clinical studies were analyzed to assess therapeutic potential, with attention to mechanisms such as cell replacement, neuroprotection, and paracrine effects. Relevant challenges, including ethical concerns and clinical translation, were also explored. Results: Cell-based therapies demonstrate potential for restoring retinal function and slowing disease progression through mechanisms like neuroprotection and cell replacement. Preclinical trials show promising outcomes, but clinical studies face significant hurdles, including challenges in cell delivery and long-term efficacy. Combination therapies integrating gene editing and biomaterials offer potential future advancements. Conclusions: While cell-based therapies for AMD and RP have made significant progress, substantial barriers to clinical application remain. Further research is essential to overcome these obstacles, improve delivery methods, and ensure the safe and effective translation of these therapies into clinical practice.
PLGA-PEG Nanoparticles Loaded with Cdc42 Inhibitor for Colorectal Cancer Targeted Therapy
Authors: Kadyr Sanazar , Zhuraliyeva Altyn , Yermekova Aislu , Makhambetova Aigerim , Kaldybekov Daulet B. , Mun Ellina A. , Bulanin Denis , Askarova Sholpan N. , Umbayev Bauyrzhan A.
Published: October 2024

Background/Objectives: An inhibitor of small Rho GTPase Cdc42, CASIN, has been shown to reduce cancer cell proliferation, migration, and invasion, yet it has several limitations, including rapid drug elimination and low bioavailability, which prevents its systemic administration. In this study, we designed and characterized a nanoparticle-based delivery system for CASIN encapsulated within poly(lactide-co-glycolide)-block-poly(ethylene glycol)-carboxylic acid endcap nanoparticles (PLGA-PEG-COOH NPs) for targeted inhibition of Cdc42 activity in colon cancer. Methods: We applied DLS, TEM, and UV–vis spectroscopy methods to characterize the size, polydispersity index, zeta potential, encapsulation efficiency, loading capacity, and in vitro drug release of the synthesized nanoparticles. The CCK-8 cell viability test was used to study colorectal cancer cell growth in vitro. Results: We showed that CASIN-PLGA-PEG-COOH NPs were smooth, spherical, and had a particle size of 86 ± 1 nm, with an encapsulation efficiency of 66 ± 5% and a drug-loading capacity of 5 ± 1%. CASIN was gradually released from NPs, reaching its peak after 24 h, and could effectively inhibit the proliferation of HT-29 (IC50 = 19.55 µM), SW620 (IC50 = 9.33 µM), and HCT116 (IC50 = 10.45 µM) cells in concentrations ranging between 0.025–0.375 mg/mL. CASIN-PLGA-PEG-COOH NPs demonstrated low hemolytic activity with a hemolytic ratio of less than 1% for all tested concentrations. Conclusion: CASIN-PLGA-PEG-COOH NPs have high encapsulation efficiency, sustained drug release, good hemocompatibility, and antitumor activity in vitro. Our results suggest that PLGA-PEG-COOH nanoparticles loaded with CASIN show potential as a targeted treatment for colorectal cancer and could be recommended for further in vivo evaluation.
Discrepancy between Subjective and Objective Measurements for the Evaluation of Medication Adherence—A Cross-Sectional Study in Patients with Cardiovascular Diseases
Authors: Miyazaki Motoyasu , Hirata Hitomi , Takaki Satoko , Misaki Momoko , Mori Yukako , Tokura Kaoko , Sato Natsuki , Nakashima Akio , Yanagida Atsuko , Okajima Isa , Urata Hidenori , Imakyure Osamu
Published: October 2024

Medication adherence is important for the appropriate drug-based treatment in patients with chronic diseases, especially those with cardiovascular diseases (CVDs). The purpose of the present study was to evaluate medication adherence among patients with CVDs using subjective and objective measurements. We enrolled outpatients who visited Fukuoka University Chikushi Hospital from June to December 2022. As a subjective measurement, we used a self-reported questionnaire developed by Ueno et al., which consists of 12 questionnaire items grouped into the following four domains: medication compliance (subjective compliance), collaboration with health care providers (collaboration), willingness to access and use information about medication (willingness), and acceptance to take medication and how taking medication fits a patient’s lifestyle (acceptance). The pill counting method was used as an objective measurement to calculate the medication adherence rate; Poor Adherence was defined as a medication adherence rate of <100%. Ninety-four patients were analyzed. No statistically significant differences were observed between the patients in the Good and Poor Adherence groups classified by pill counting, an objective indicator; in the subjective evaluation index Ueno scale scores of subjective compliance, collaboration, willingness, and acceptance domains; and in the total score. A multivariate analysis revealed that obesity (odds ratio, 3.527; 95% confidence interval, 1.387–9.423; p = 0.008) was an independent factor associated with Poor Adherence. In conclusion, we found a discrepancy between subjective and objective measurements for the evaluation of medication adherence. Furthermore, obesity was an independent factor associated with poor medication adherence assessed by the pill counting method; thus, patients with CVD and obesity require a careful follow-up.
Combined Efficacy of Q-Switched 785 nm Laser and Tranexamic Acid Cream in the Treatment of Melasma: A Prospective Clinical Study
Authors: Zappia Elena , Piccolo Domenico , Del Re Chiara , Bonan Paolo , Guarino Luca , Ribero Simone , Galadari Hassan , Nisticò Steven Paul
Published: October 2024

Background and Objectives: Melasma, a hypermelanotic dermatologic condition that mainly affects women, poses challenges due to its complex etiology involving environmental and genetic factors. Its pathophysiology, marked by intricate histological changes, is not fully understood. This study explored the efficacy and safety of a new 785 nm picosecond laser in treating facial melasma. Materials and Methods: An 11-participant cohort, comprising women with Fitzpatrick phototypes II-III, underwent a treatment protocol with a new 785 nm picosecond laser. The clinical evaluation used the Global Aesthetic International Score (GAIS) and the Five-Point Likert Scale Questionnaire. The aim of the study was to understand the capacity of the 785 nm wavelength laser to interact with both the pigmentary and vascular components of melasma. Results: The GAIS outcomes revealed excellent (18.2%), good (54.5%), poor (18.2%), and no results (9.1%). The Likert Scale responses varied from very satisfied (18.2%) to slightly satisfied (9.1%). Clinical images at three months demonstrated resolution of melasma with no adverse events. Conclusions: This non-invasive procedure showed positive outcomes and high patient tolerance, emphasizing its potential in melasma management. However, in order to fully understand the interactions of pigmentary and vascular components with the 785 nm wavelength laser, further research is required. The small cohort represents a limitation for this study, therefore studies that include a larger number of patients are needed to assess the effectiveness of this laser treatment for facial melasma.
An Ultra-Wideband Metamaterial Absorber Ranging from Near-Infrared to Mid-Infrared
Authors: Lin Jing-Jenn , Huang Dun-Yu , Hong Meng-Long , Huang Jo-Ling , Wang Chih-Hsuan , Yang Cheng-Fu , Lai Kuei-Kuei
Published: October 2024

This study focused on designing an ultra-wideband metamaterial absorber, consisting of layers of Mn (manganese) and MoO3 (molybdenum trioxide) arranged in a planar interleaving pattern, with a matrix square-shaped Ti (titanium) on the top MoO3 layer. Key features of this research included the novel use of Mn and MoO3 in a planar interleaving configuration for designing an ultra-wideband absorber, which was rarely explored in previous studies. MoO3 thin film served as the fundamental material, leveraging its favorable optical properties and absorption capabilities in the infrared spectrum. Alternating layers of Mn and MoO3 were adjusted in thickness and order to optimize absorptivity across desired wavelength ranges. Another feature is that the Mn and MoO3 materials in the investigated absorber had a planar structure, which simplified the manufacturing of the absorber. Furthermore, the topmost layer of square-shaped Ti was strategically placed to enhance the absorber’s bandwidth and efficiency. When the investigated absorber lacked a Ti layer, its absorptivity and bandwidth significantly decreased. This structural design leveraged the optical properties of Mn, MoO3, and Ti to significantly expand the absorption range across an ultra-wideband spectrum. When the Ti height was 280 nm, the investigated absorber exhibited a bandwidth with absorptivity greater than 0.9, spanning from the near-infrared (0.80 μm) to the mid-infrared (9.07 μm). The average absorptivity in this range was 0.950 with a maximum absorptivity of 0.989. Additionally, three absorption peaks were observed at 1010, 2510, and 6580 nm. This broad absorption capability makes it suitable for a variety of optical applications, ranging from near-infrared to mid-infrared wavelengths, including thermal imaging and optical sensing.
NOMA Visible Light Communications with Distinct Optical Beam Configurations
Authors: Ding Jupeng , I Chih-Lin , Wang Jintao , Yang Hui
Published: October 2024

Visible light communication (VLC) has been viewed as one promising candidate to mitigate the challenging spectrum crisis and radio frequency interference in future 6G mobile communications and networking. Due to the relatively limited baseband modulation bandwidth of VLC light sources—typically, light-emitting diodes—non-orthogonal multiple access (NOMA) techniques have been proposed and explored to enhance the spectral efficiency (SE) of VLC systems. However, almost all reported NOMA VLC schemes focus on well-discussed applications employing a Lambertian light beam configuration and ignore the potential applications with distinct non-Lambertian optical beam configurations. To address this issue, in this work, the performance of non-Lambertian optical beam configuration-based NOMA VLC is comparatively investigated for future 6G mobile networks. The numerical results demonstrate that, for a fundamental two-user application scenario with the far user located at the corner position, a maximum sum rate gain of about 15.6 Mbps could be provided by the investigated distinct non-Lambertian beam-based NOMA VLC, compared with the maximum sum rate of about 93.3 Mbps for the conventional Lambertian configuration with the same power splitting factor.
Braiding Fruits and Flowers as a Wish of Prosperity and Victory over Death in the Carved Festoons of Ancient Rome
Authors: Lazzara Alessandro , Kumbaric Alma , Pergola Agnese , Caneva Giulia
Published: October 2024

Plant motifs had a significant role in ancient cultures, with decorative, artistic, and communicative values. However, little knowledge exists of the botanical composition of festoons, widely used in Greek-Roman art. We analysed 81 festoons, exclusively from sculpture artworks, collected from 13 museums and archaeological sites in Rome (1st century BC–3rd century AD). Using iconographic sources and previous data, we identified the represented species and analysed their abundance and composition using statistical methods (Cluster Analysis, Principal Components Analysis) and diversity indexes (Shannon and Evenness). We documented 3081 botanical elements, identifying 30 taxa, in which fruits with leaves (45%) or alone (10%) represented the most common ones. Laurus nobilis and Quercus cfr. robur were the most frequently depicted species, followed by “pomes” (Pyrus, Malus, Cydonia), Vitis vinifera, Punica granatum, and Ficus carica. Festoons with one or two species can be easily distinguished from those with multiple species, often arranged with figs or vine branches at the ends, with symbolic aims related to fertility, rebirth, and abundance values. A balanced botanical composition also exists, with flowers typically in the middle and a species distribution that is not casual. The results enriched our comprehension of ancient Roman society, considering funerary and celebrative events.
Comparative Physiological, Biochemical, and Leaf Proteome Responses of Contrasting Wheat Varieties to Drought Stress
Authors: Moloi Sellwane J. , Alqarni Ali O. , Brown Adrian P. , Goche Tatenda , Shargie Nemera G. , Moloi Makoena J. , Gokul Arun , Chivasa Stephen , Ngara Rudo
Published: October 2024

Drought stress severely affects crop productivity and threatens food security. As current trends of global warming are predicted to exacerbate droughts, developing drought-resilient crops becomes urgent. Here, we used the drought-tolerant (BW35695) and drought-sensitive (BW4074) wheat varieties to investigate the physiological, biochemical, and leaf proteome responses underpinning drought tolerance. In response to drought, the tolerant variety had higher osmolyte accumulation and maintained higher leaf water content than the sensitive variety. BW35695 also had an enhanced antioxidant enzyme capacity and reduced reactive oxygen species (ROS), resulting in diminished membrane lipid damage, as reflected by malondialdehyde content. Proteomic analysis revealed that drought-induced differential expression of proteins involved in diverse biological processes in both wheat varieties, including primary and secondary metabolism, protein synthesis/folding/degradation, defense/ROS detoxification, energy, transcription, and cell structure. Notably, photosynthesis emerged as the most enriched biochemical process targeted for suppression in the drought-tolerant BW35695 wheat, but not in drought-sensitive BW4074, possibly as a survival strategy for averting cell damage inflicted by photosynthesis-derived ROS. Additionally, protein synthesis-related proteins were highly upregulated in BW35695, presumably to drive cell-wide stress-adaptive responses. The protein network identified here will be useful in further studies to understand the molecular basis for divergent drought response phenotypes in crops.