bluuebunny/crossref_metadata_2025 huggingface dataset:
Stenotrophomonas maltophilia natural history and evolution in the airways of adults with cystic fibrosis
Authors: Izydorczyk Conrad , Waddell Barbara J. , Thornton Christina S. , Conly John M. , Rabin Harvey R. , Somayaji Ranjani , Surette Michael G. , Church Deirdre L. , Parkins Michael D.
Published: June 2023

Introduction

Stenotrophomonas maltophilia is an opportunistic pathogen infecting persons with cystic fibrosis (pwCF) and portends a worse prognosis. Studies of S. maltophilia infection dynamics have been limited by cohort size and follow-up. We investigated the natural history, transmission potential, and evolution of S. maltophilia in a large Canadian cohort of 321 pwCF over a 37-year period.

Methods

One-hundred sixty-two isolates from 74 pwCF (23%) were typed by pulsed-field gel electrophoresis, and shared pulsotypes underwent whole-genome sequencing.

Results

S. maltophilia was recovered at least once in 82 pwCF (25.5%). Sixty-four pwCF were infected by unique pulsotypes, but shared pulsotypes were observed between 10 pwCF. In chronic carriage, longer time periods between positive sputum cultures increased the likelihood that subsequent isolates were unrelated. Isolates from individual pwCF were largely clonal, with differences in gene content being the primary source of genetic diversity objectified by gene content differences. Disproportionate progression of CF lung disease was not observed amongst those infected with multiple strains over time (versus a single) or amongst those with shared clones (versus strains only infecting one patient). We did not observe evidence of patient-to-patient transmission despite relatedness between isolates. Twenty-four genes with ≥ 2 mutations accumulated over time were identified across 42 sequenced isolates from all 11 pwCF with ≥ 2 sequenced isolates, suggesting a potential role for these genes in adaptation of S. maltophilia to the CF lung.

Discussion

Genomic analyses suggested common, indirect sources as the origins of S. maltophilia infections in the clinic population. The information derived from a genomics-based understanding of the natural history of S. maltophilia infection within CF provides unique insight into its potential for in-host evolution.
Artificial mixed microbial system for polycyclic aromatic hydrocarbons degradation
Authors: Cui Jia-Qi , He Zhi-Qiang , Ntakirutimana Samuel , Liu Zhi-Hua , Li Bing-Zhi , Yuan Ying-Jin
Published: June 2023

Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants with major risks to human health. Biological degradation is environmentally friendly and the most appealing remediation method for a wide range of persistent pollutants. Meanwhile, due to the large microbial strain collection and multiple metabolic pathways, PAH degradation via an artificial mixed microbial system (MMS) has emerged and is regarded as a promising bioremediation approach. The artificial MMS construction by simplifying the community structure, clarifying the labor division, and streamlining the metabolic flux has shown tremendous efficiency. This review describes the construction principles, influencing factors, and enhancement strategies of artificial MMS for PAH degradation. In addition, we identify the challenges and future opportunities for the development of MMS toward new or upgraded high-performance applications.
Diagnostic utility of movement disorder society criteria for multiple system atrophy
Authors: Zhang Lingyu , Hou Yanbing , Wei Qianqian , Ou Ruwei , Liu Kuncheng , Lin Junyu , Yang Tianmi , Xiao Yi , Zhao Bi , Shang Huifang
Published: June 2023

Background

The 2008 criteria for the diagnosis of multiple system atrophy (MSA) has been widely used for more than 10 years, but the sensitivity is low, particularly for patients in the early stage. Recently, a new MSA diagnostic criteria was developed.

Objective

The objective of the study was to assess and compare the diagnostic utility of the new movement disorder society (MDS) MSA criteria with the 2008 MSA criteria.

Methods

This study included patients diagnosed with MSA between January 2016 and October 2021. All patients underwent regular face-to-face or telephonic follow-ups every year until October 2022. A total of 587 patients (309 males and 278 females) were retrospectively reviewed to compare the diagnostic accuracy of the MDS MSA criteria to that of the 2008 MSA criteria (determined by the proportion of patients categorized as established or probable MSA). Autopsy is the gold standard diagnosis of MSA, which is not available in clinical practice. Thus, we applied the 2008 MSA criteria at the last review as the reference standard.

Results

The sensitivity of the MDS MSA criteria (93.2%, 95% CI = 90.5–95.2%) was significantly higher than that of the 2008 MSA criteria (83.5%, 95% CI = 79.8–86.6%) (P < 0.001). Additionally, the sensitivity of the MDS MSA criteria was maintained robustly across different subgroups, defined by diagnostic subtype, disease duration, and the type of symptom[s] at onset. Importantly, the specificities were not significantly different between the MDS MSA criteria and the 2008 MSA criteria (P > 0.05).

Conclusion

The present study demonstrated that the MDS MSA criteria exhibited good diagnostic utility for MSA. The new MDS MSA criteria should be considered as a useful diagnostic tool for clinical practice and future therapeutic trials.
mEMbrain: an interactive deep learning MATLAB tool for connectomic segmentation on commodity desktops
Authors: Pavarino Elisa C. , Yang Emma , Dhanyasi Nagaraju , Wang Mona D. , Bidel Flavie , Lu Xiaotang , Yang Fuming , Francisco Park Core , Bangalore Renuka Mukesh , Drescher Brandon , Samuel Aravinthan D. T. , Hochner Binyamin , Katz Paul S. , Zhen Mei , Lichtman Jeff W. , Meirovitch Yaron
Published: June 2023

Connectomics is fundamental in propelling our understanding of the nervous system's organization, unearthing cells and wiring diagrams reconstructed from volume electron microscopy (EM) datasets. Such reconstructions, on the one hand, have benefited from ever more precise automatic segmentation methods, which leverage sophisticated deep learning architectures and advanced machine learning algorithms. On the other hand, the field of neuroscience at large, and of image processing in particular, has manifested a need for user-friendly and open source tools which enable the community to carry out advanced analyses. In line with this second vein, here we propose mEMbrain, an interactive MATLAB-based software which wraps algorithms and functions that enable labeling and segmentation of electron microscopy datasets in a user-friendly user interface compatible with Linux and Windows. Through its integration as an API to the volume annotation and segmentation tool VAST, mEMbrain encompasses functions for ground truth generation, image preprocessing, training of deep neural networks, and on-the-fly predictions for proofreading and evaluation. The final goals of our tool are to expedite manual labeling efforts and to harness MATLAB users with an array of semi-automatic approaches for instance segmentation. We tested our tool on a variety of datasets that span different species at various scales, regions of the nervous system and developmental stages. To further expedite research in connectomics, we provide an EM resource of ground truth annotation from four different animals and five datasets, amounting to around 180 h of expert annotations, yielding more than 1.2 GB of annotated EM images. In addition, we provide a set of four pre-trained networks for said datasets. All tools are available from https://lichtman.rc.fas.harvard.edu/mEMbrain/. With our software, our hope is to provide a solution for lab-based neural reconstructions which does not require coding by the user, thus paving the way to affordable connectomics.
Insomnia and circadian rhythm: a bibliometrics study and visualization analysis via CiteSpace
Authors: He Qing-Yun , Dai Ning , Mao Meng , Ma Jie , Wen Qiao , Song Dan-Dan , Liu Yan , Li Feng
Published: June 2023

Objective

The present study aimed to use CiteSpace to analyze the status of insomnia and circadian rhythm, identify the hot spots and trends, and provide a basis for future study.

Method

The Web of Science database was searched for studies related to insomnia and circadian from its inception to 14 April 2023. CiteSpace was used to generate online maps of collaboration between countries and authors and revealed hot spots and frontiers in insomnia and circadian rhythm.

Results

We searched 4,696 publications related to insomnia and circadian rhythm. Bruno Etain was the most prolific author with most publications, i.e., with 24 articles. The USA and the University of California were the leading country and the top institution in this field of study, with 1,672 and 269 articles, respectively. There was active cooperation between institutions, countries, and authors. Hot topics focused on circadian rhythm sleep disorders, circadian clock, light therapy, melatonin, and bipolar disorder.

Conclusion

Based on the CiteSpace results, we recommend a more active collaboration between various countries, institutions, and authors to conduct clinical and basic research related to insomnia and circadian rhythm. Ongoing research focuses on the interaction of insomnia with circadian rhythms and the corresponding pathways of clock genes and by extension, the role of circadian rhythms in disorders such as bipolar disorder. Modulation of circadian rhythms may be a hot spot for future insomnia therapies (such as light therapy and melatonin).
Walking performance of persons with chronic stroke changed when looking down but not in dimly lit environment
Authors: Lee Pei-Yun , Hseuh Yu-Chu , Chen Chih-Hung , Lin Sang-I
Published: June 2023

Background and purpose

It is common to walk under different conditions, such as looking straight head, looking down at the feet or in dimly lit environment. The purpose of this study was to determine the impact of these different conditions on walking performance in persons with and without stroke.

Methods

This was a case-control study. Persons with chronic unilateral stroke and age-matched control (n = 29 each) underwent visual acuity test, Mini Mental Status Examination (MMSE) and joint position sense test of the knee and ankle. The participants walked at their preferred speed under three walking conditions, looking ahead (AHD), looking down (DWN), and in dimly lit environment (DIM). A motion analysis system was used for the recording of the limb matching test and walking tasks.

Results

Stroke participants differed from the control group in MMSE, but not in age, visual acuity or joint position sense. For the control group, the differences between the three walking conditions were nonsignificant. For the stroke group, DWN had significantly slower walking speed, greater step width and shorter single leg support phase, but not different symmetry index or COM location, compared to AHD. The differences between AHD and DIM were nonsignificant.

Conclusion

Healthy adults did not change their gait patterns under the different walking conditions. Persons with chronic stroke walked more cautiously but not more symmetrically when looking down at the feet, but not in dimly lit environment. Ambulatory persons with stroke may need to be advised that looking down at the feet while walking could be more challenging.
The advantages of the magnetic resonance image compilation (MAGiC) method for the prognosis of neonatal hypoglycemic encephalopathy
Authors: Tian Zhongfu , Zhu Qing , Wang Ruizhu , Xi Yanli , Tang Wenwei , Yang Ming
Published: June 2023

Objectives

To explore the prognostic value of magnetic resonance image compilation (MAGiC) in the quantitative assessment of neonatal hypoglycemic encephalopathy (HE).

Methods

A total of 75 neonatal HE patients who underwent synthetic MRI were included in this retrospective study. Perinatal clinical data were collected. T1, T2 and proton density (PD) values were measured in the white matter of the frontal lobe, parietal lobe, temporal lobe and occipital lobe, centrum semiovale, periventricular white matter, thalamus, lenticular nucleus, caudate nucleus, corpus callosum and cerebellum, which were generated by MAGiC. The patients were divided into two groups (group A: normal and mild developmental disability; group B: severe developmental disability) according to the score of Bayley Scales of Infant Development (Bayley III) at 9–12 months of age. Student’s t test, Wilcoxon test, and Fisher’s test were performed to compare data across the two groups. Multivariate logistic regression was used to identify the predictors of poor prognosis, and receiver operating characteristic (ROC) curves were created to evaluate the diagnostic accuracy.

Results

T1 and T2 values of the parietal lobe, occipital lobe, center semiovale, periventricular white matter, thalamus, and corpus callosum were higher in group B than in group A (p < 0.05). PD values of the occipital lobe, center semiovale, thalamus, and corpus callosum were higher in group B than in group A (p < 0.05). Multivariate logistic regression analysis showed that the duration of hypoglycemia, neonatal behavioral neurological assessment (NBNA) scores, T1 and T2 values of the occipital lobe, and T1 values of the corpus callosum and thalamus were independent predictors of severe HE (OR > 1, p < 0.05). The T2 values of the occipital lobe showed the best diagnostic performance, with an AUC value of 0.844, sensitivity of 83.02%, and specificity of 88.16%. Furthermore, the combination of MAGiC quantitative values and perinatal clinical features can improve the AUC (AUC = 0.923) compared with the use of MAGiC or perinatal clinical features alone.

Conclusion

The quantitative values of MAGiC can predict the prognosis of HE early, and the prediction efficiency is further optimized after being combined with clinical features.
N-methyl-D-aspartate receptor hypofunction as a potential contributor to the progression and manifestation of many neurological disorders
Authors: Dong Bin , Yue Yang , Dong Han , Wang Yuehui
Published: June 2023

N-methyl-D-aspartate receptors (NMDA) are glutamate-gated ion channels critical for synaptic transmission and plasticity. A slight variation of NMDAR expression and function can result in devastating consequences, and both hyperactivation and hypoactivation of NMDARs are detrimental to neural function. Compared to NMDAR hyperfunction, NMDAR hypofunction is widely implicated in many neurological disorders, such as intellectual disability, autism, schizophrenia, and age-related cognitive decline. Additionally, NMDAR hypofunction is associated with the progression and manifestation of these diseases. Here, we review the underlying mechanisms of NMDAR hypofunction in the progression of these neurological disorders and highlight that targeting NMDAR hypofunction is a promising therapeutic intervention in some neurological disorders.
Human blood plasma biomarkers of diet and weight loss among centrally obese subjects in a New Nordic Diet intervention
Authors: Trimigno Alessia , Khakimov Bekzod , Rasmussen Morten Arendt , Dragsted Lars Ove , Larsen Thomas Meinert , Astrup Arne , Engelsen Søren Balling
Published: June 2023

Scope

The New Nordic Diet (NND) has been shown to promote weight loss and lower blood pressure amongst obese people. This study investigates blood plasma metabolite and lipoprotein biomarkers differentiating subjects who followed Average Danish Diet (ADD) or NND. The study also evaluates how the individual response to the diet is reflected in the metabolic differences between NND subjects who lost or maintained their pre-intervention weight.

Methods

Centrally obese Danes (BMI >25) followed NND (90 subjects) or ADD (56 subjects) for 6 months. Fasting blood plasma samples, collected at three time-points during the intervention, were screened for metabolites and lipoproteins (LPs) using proton nuclear magnetic resonance spectroscopy. In total, 154 metabolites and 65 lipoproteins were analysed.

Results

The NND showed a relatively small but significant effect on the plasma metabolome and lipoprotein profiles, with explained variations ranging from 0.6% for lipoproteins to 4.8% for metabolites. A total of 38 metabolites and 11 lipoproteins were found to be affected by the NND. The primary biomarkers differentiating the two diets were found to be HDL-1 cholesterol, apolipoprotein A1, phospholipids, and ketone bodies (3-hydroxybutyric acid, acetone, and acetoacetic acid). The increased levels of ketone bodies detected in the NND group inversely associated with the decrease in diastolic blood pressure of the NND subjects. The study also showed that body weight loss among the NND subjects was weakly associated with plasma levels of citrate.

Conclusion

The main plasma metabolites associated with NND were acetate, methanol and 3-hydroxybutyrate. The metabolic changes associated with the NND-driven weight loss are mostly pronounced in energy and lipid metabolism.
Checkpoint inhibitors as dual immunotherapy in advanced non-small cell lung cancer: a meta-analysis
Authors: Alifu Muyesar , Tao Min , Chen Xiao , Chen Jie , Tang Kejing , Tang Yubo
Published: June 2023

Introduction

Recent clinical trials have confirmed that anti-programmed cell death-1/ligand 1 (anti-PD-1/L1) combined with either anti-cytotoxic T-lymphocyte-associated protein 4 (anti-CTLA-4) or anti-T-cell immunoreceptor with Ig and ITIM domains (TIGIT) antibodies (dual immunotherapy) produced significant benefits as first-line therapies for patients with advanced non-small cell lung cancer (NSCLC). However, it also increased the incidence of adverse reactions, which cannot be ignored. Our study aims to explore the efficacy and safety of dual immunotherapies in advanced NSCLC.

Methods

This meta-analysis ultimately included nine first-line randomized controlled trials collected from PubMed, EMBASE, and Cochrane Central Register of Controlled Trials databases until 13 August 2022. Efficacy was measured as the hazard ratio (HR) and 95% confidence interval (CI) for progression-free survival (PFS), overall survival (OS), and risk ratio (RR) for the objective response rates (ORRs). Treatment safety was assessed by RR of any grade of treatment-related adverse events (TRAEs) and grade ≥ 3 TRAEs.

Results

Our results demonstrated that, compared to chemotherapy, dual immunotherapy shows durable benefits in OS (HR = 0.76, 95% CI: 0.69–0.82) and PFS (HR = 0.75, 95% CI: 0.67–0.83) across all levels of PD-L1 expression. Subgroup analysis also presented that dual immunotherapy resulted in improved long-term survival compared with chemotherapy in patients with a high tumor mutational burden (TMB) (OS: HR = 0.76, p = 0.0009; PFS: HR = 0.72, p < 0.0001) and squamous cell histology (OS: HR = 0.64, p < 0.00001; PFS: HR = 0.66, p < 0.001). However, compared with immune checkpoint inhibitor (ICI) monotherapy, dual immunotherapy shows some advantages in terms of OS and ORR and only improved PFS (HR = 0.77, p = 0.005) in PD-L1 < 25%. With regard to safety, there was no significant difference in any grade TRAEs (p = 0.05) and grade ≥ 3 TRAEs (p = 0.31) between the dual immunotherapy and chemotherapy groups. However, compared with ICI monotherapy, dual immunotherapy significantly increased the incidence of any grade TRAEs (p = 0.03) and grade ≥ 3 TRAEs (p < 0.0001).

Conclusions

As for the efficacy and safety outcome, compared with standard chemotherapy, dual immunotherapy remains an effective first-line therapy for patients with advanced NSCLC, especially for patients with high TMB levels and squamous cell histology. Furthermore, compared to single-agent immunotherapy, dual immunotherapy is only considered for use in patients with low PD-L1 expression in order to reduce the emergence of resistance to immunotherapy.

Systematic Review Registation

https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42022336614.
Case Report: Severe cutaneous adverse event associated with checkpoint inhibition in the setting of CAR T-cell therapy: beyond CRS
Authors: Masucci Chiara , Pepe Sara , La Rocca Ursula , Zullino Veronica , De Propris Maria Stefania , Barberi Walter , Iori Anna Paola , Martelli Sabina , Ruberto Franco , Martelli Maurizio , Di Rocco Alice
Published: June 2023

Anti-CD19 chimeric antigen receptor (CAR) T cell therapy actually represents the standard of care for multiple relapsed or refractory primary mediastinal B-cell lymphoma (r/r PMBCL). Checkpoint inhibitors, such as pembrolizumab, appear to be a safe and effective treatment strategy for patients who are ineligible for or resistant to autologous stem cell transplantation. Although preclinical studies suggested that checkpoint inhibitors may enhance the vitality and anti-tumor activity of CAR T cells, there are no substantial/robust clinical data about the immune-mediated toxicity of their association. We describe a case of a severe cutaneous adverse event arising immediately after Cytokine Release Syndrome (CRS) on day +6 from CAR T cells infusion in a young r/r PMBCL patient who previously received pembrolizumab. These skin lesions were interpreted as an immune mediated adverse event, considering their prompt improvement and fully recovering achieved with the addition of immunoglobulin infusion to systemic steroid therapy. This case of life-threatening cutaneous adverse event calls for further investigations about off-target immune-related adverse events deriving from the combination of CAR T cell therapy and checkpoint inhibition, whose synergic therapeutic effect is promising.
A preliminary investigation of the relationship between 18F-FDG PET/CT metabolic parameters and prognosis in angioimmunoblastic T-cell lymphoma
Authors: Hu Lanping , Luo Nana , Li Lei , Qiu Dasheng , Hu Xiaoyan
Published: June 2023

Purpose

The goal of the study was to determine the prognostic significance of metabolic parameters in baseline 18F-FDG PET/CT images obtained from patients with angioimmunoblastic T-cell lymphoma (AITL).

Methods

Forty patients with pathologically diagnosed AITL who had baseline 18F-FDG PET/CT between May 2014 and May 2021 were assessed as part of this study. Maximum standardized uptake value (SUVmax), total lesion glycolysis (TLG), and total metabolic tumor volume (TMTV) were obtained and analyzed. In addition, many relevant features were evaluated, including sex, age, staging, International Prognostic Index (IPI), prediction index for T-cell lymphoma (PIT), Ki-67, and so on. Estimates of progression-free survival (PFS) and overall survival (OS) were determined using the log-rank test and Kaplan-Meier.

Results

The median follow-up was 30.2 months (interquartile range 9.82-43.03). Throughout the follow-up period, 29 (72.5%) deaths occurred and 22 (55.0%) patients made progress. The rates for 2- and 3-year PFS were 43.6% and 26.4%, respectively. The 3- and 5-year OS were 42.6% and 21.5%. For TMTV, TLG, and SUVmax, the cut-off values were 87.0 cm3, 711.1, and 15.8, respectively. Poorer PFS and OS were substantially correlated with high SUVmax and TLG. An increased TMTV suggested a shorter OS. TLG performed independently as OS predictors in multivariate analysis. The risk score for predicting the prognosis of AITL includes the TMTV, TLG, SUVmax, and IPI scores, with 4.5 for TMTV, 2 for TLG, 1.5 for IPI scores, and 1 for SUVmax. Three risk categories of patients with AITL had 3-year OS rates of 100.0%, 43.3%, and 25.0%, respectively.

Conclusion

Baseline TLG was a strong predictor of OS. Here a new prognostic scoring system for AITL based on the clinical indicators and PET/CT metabolic parameters was constructed, which might make stratification of prognosis easy and also help to individualize treatment.
The diagnosis and treatment of testicular torsion in children with non-scrotal initial symptoms
Authors: Mao Chang-Kun , Cao Yong-Sheng , Liu Xiang , Peng Bo , Chu Han , Deng Qi-Fei , Yu Xin , Tao Cheng-Pin , Zhang Tao , Yang Chao , Peng Shan
Published: June 2023

Objective

To explore the clinical characteristics of testicular torsion in children with non-scrotal initial symptoms who were misdiagnosed.

Methods

A retrospective analysis of 73 cases children with testicular torsion and non-scrotal symptoms who were admitted to our department from October 2013 to December 2021 was performed. Patients were divided into misdiagnosis (27 cases) and clear diagnosis at first visit (46 cases) groups. Clinical data, including age at surgery, clinical presentation, physical examination, number of visits (≥2 times), affected side, time from initial symptoms to surgery, and surgical outcomes, were collected. The TWIST (Testicular Workup for Ischemia and Suspected Torsion) score was calculated and analyzed.

Results

Statistically significant differences between the misdiagnosis and clear diagnosis groups were seen in the time from initial symptoms to surgery, the number of visits, the degree of testicular torsion, and the rate of orchiectomy (P < 0.05). There were no statistically significant differences (P > 0.05) in age, affected side, TWIST score, guardian, direction of testicular torsion, intra-vaginal or extra-vaginal torsion, and Arda classification. Postoperative follow-up was 6–40 months. Of the 36 patients who required an orchiopexy, 1 had testicular atrophy at six months and 2 were lost to follow-up. The contralateral testis of the 37 children who underwent orchiectomies developed normally without torsion.

Conclusions

The clinical manifestations of testicular torsion in children are diverse and can easily lead to misdiagnosis. Guardians should be aware of this pathology and seek timely medical attention. When the initial diagnosis and treatment of testicular torsion is difficult, the TWIST score during the physical examination may be useful, especially for patients with intermediate-to-high risk scores. Color Doppler ultrasound can assist in making the diagnosis, but when testicular torsion is highly suspected, routine ultrasound is not necessary as it may lead to delayed surgical treatment.
Case report: marfan syndrome (MFS) mimicking cutaneous vasculitis
Authors: Price-Kuehne Fiona , Omoyinmi Ebun , Younes Maha , Edwards Matthew , Eleftheriou Despina , Brogan Paul
Published: June 2023

Marfan syndrome (MFS) is an autosomal dominant connective tissue disorder caused by variants in the extracellular microfibril fibrillin (FBN1) gene. Here we report an FBN1 variant in a child with an unusual skin rash mimicking cutaneous vasculitis, and mild aortic root dilatation. The case was complicated by lack of typical skeletal MFS phenotype; and severe needle phobia preventing any blood testing for workup of suspected vasculitis. Therefore inflammatory markers, autoantibody profile and general hematology/biochemistry results were unknown. Diagnosis of MFS was made via genetic testing of a saliva sample alone using a next-generation sequencing (NGS) targeted gene panel designed to screen for monogenic forms of vasculitis and noninflammatory vasculopathic mimics. This revealed the patient was heterozygous for a pathogenic frameshift variant in FBN1; NM_000138, c.1211delC, p.(Pro404Hisfs*44), predicted to cause premature protein truncation leading to loss of function. The variant has not been detected in control populations and has previously been detected in individuals with MFS. This rapid diagnosis significantly impacted the patient management: avoidance of invasive investigations; avoidance of unnecessary immunosuppression; facilitating genetic counselling of the index case and family; and directly informing lifelong monitoring and ongoing treatment for aortic root involvement from MFS. This case further emphasizes the diagnostic utility of NGS early in the diagnostic workup of paediatric patients referred with suspected vasculitis, and we emphasize that MFS can present with cutaneous vasculitic-like features in the absence of the typical Marfanoid skeletal phenotype.
Lanatoside C decelerates proliferation and induces apoptosis through inhibition of STAT3 and ROS-mediated mitochondrial membrane potential transformation in cholangiocarcinoma
Authors: Zhang Chao , Yang Hong-Ying , Gao Long , Bai Ming-Zhen , Fu Wen-Kang , Huang Chong-Fei , Mi Ning-Ning , Ma Hai-Dong , Lu Ya-Wen , Jiang Ning-Zu , Tian Liang , Cai Teng , Lin Yan-Yan , Zheng Xing-Xing , Gao Kun , Chen Jian-Jun , Meng Wen-Bo
Published: June 2023

Introduction: The incidence of cholangiocarcinoma (CCA) has increased worldwide in recent years. Given the poor prognosis associated with the current management approach of CCA, new therapeutic agents are warranted to improve the prognosis of this patient population.

Methods: In this study, we extracted five cardiac glycosides (CGs) from natural plants: digoxin, lanatoside A, lanatoside C, lanatoside B, and gitoxin. Follow-up experiments were performed to assess the effect of these five extracts on cholangiocarcinoma cells and compounds with the best efficacy were selected. Lanatoside C (Lan C) was selected as the most potent natural extract for subsequent experiments. We explored the potential mechanism underlying the anticancer activity of Lan C on cholangiocarcinoma cells by flow cytometry, western blot, immunofluorescence, transcriptomics sequencing, network pharmacology and in vivo experiments.

Results: We found that Lan C time-dependently inhibited the growth and induced apoptosis of HuCCT-1 and TFK-1 cholangiocarcinoma cells. Besides Lan C increased the reactive oxygen species (ROS) content in cholangiocarcinoma cells, decreased the mitochondrial membrane potential (MMP) and resulted in apoptosis. Besides, Lan C downregulated the protein expression of STAT3, leading to decreased expression of Bcl-2 and Bcl-xl, increased expression of Bax, activation of caspase-3, and initiation of apoptosis. N-acetyl-L-cysteine (NAC) pretreatment reversed the effect of Lan C. In vivo, we found that Lan C inhibited the growth of cholangiocarcinoma xenografts without toxic effects on normal cells. Tumor immunohistochemistry showed that nude mice transplanted with human cholangiocarcinoma cells treated with Lan C exhibited decreased STAT3 expression and increased caspase-9 and caspase-3 expression in tumors, consistent with the in vitro results.

Conclusion: In summary, our results substantiates that cardiac glycosides have strong anti-CCA effects. Interestingly the biological activity of Lan C provides a new anticancer candidate for the treatment of cholangiocarcinoma.
Geomagnetic field effects on the performance of 8-inch dynode photomultiplier tubes
Authors: Fu Zaiwei , Gao Feng , Wang Wenwen , Xia Jingkai
Published: June 2023

Two different systems have been built to study the geomagnetic field effect on the performance of large area photomultiplier tubes. The main characteristics of photomultiplier tubes such as gain, collection efficiency, transition time spread and energy resolution were measured to investigate the effect. This study shows that both the dynode structure and the orientation of photomultiplier tubes have a large influence on the performance due to the geomagnetic field effect. Different methods were proposed and tested to reduce these effects. The results were obtained with and without high-permeability permalloy foil shielding. It is found that the effect of the geomagnetic field can be significantly reduced by the permalloy foil. The influence of the geomagnetic field under different installation geometries is also studied and discussed in this paper.
Sepsis associated with acute lung injury over the period 2012–2021: a bibliometric analysis
Authors: Liang Guibin , Wang Wenhua , He Zhihui
Published: June 2023

Background: Sepsis associated with acute lung injury (ALI) is a common acute and severe disease with severe socioeconomic burden. The aim of this study is to explore the literatures of sepsis associated with ALI from a bibliometric perspective.

Methods: Articles and reviews related to sepsis associated with ALI published from 2012 to 2021 in the Web of Science Core Collection were retrieved. Countries, affiliations, journals, authors, references, co-citation and keyword analysis in this field were visually analyzed using WOS citation reports, bibliometric.com, CtieSpace and VOSviewer software.

Results: Over the last decade (2012–2021), marked progress has been made in the area of sepsis associated with ALI research. 836 papers were enrolled in this study. China accounts for the most contributors. Articles from the United States has the highest average cited. Shanghai Jiao Tong University, University of California System and Huazhong University of Science Technology were the main contributing institutions. Articles in International Immunopharmacology, Inflammation, Shock and Critical Care were cited the most. Matthay MA and Ware LB were the main contributors to this field. Inflammation and NF-κB have always been the focus of sepsis associated with ALI related research, and programmed cell death (including apoptosis, necroptosis and pyroptosis) may be the important direction of future research.

Conclusion: Research on the sepsis associated with ALI is flourishing. The research on programmed cell death is a hot spot and may be a promising research field in the coming years.
Genome-wide analysis revealed the stepwise origin and functional diversification of HSDs from lower to higher plant species
Authors: Saleem Noor , Aziz Usman , Ali Muhammad , Liu Xiangling , Alwutayd Khairiah Mubarak , Alshegaihi Rana M. , Niedbała Gniewko , Elkelish Amr , Zhang Meng
Published: June 2023

Hydroxysteroid dehydrogenase (HSDs) is an oil-body sterol protein (steroleosin) with an NADP(H) binding domain that belongs to the short-chain dehydrogenase/reductase (SDR) superfamily. There are numerous studies on the characterization of HSDs in plants. However, thus far, the evolutionary differentiation and divergence analysis of these genes remain to be explored. The current study used an integrated method to elucidate the sequential evolution of HSDs in 64 sequenced plant genomes. Analyses were conducted on their origins, distribution, duplication, evolutionary paths, domain functions, motif composition, properties, and cis-elements. Results indicate that except for algae, HSD1 was widely distributed in plant species ranging from lower to higher plants, while HSD5 was restricted to terrestrial plants, and HSD2 was identified in fewer monocots and several dicot plants. Phylogenetic analysis of HSD proteins revealed that monocotyledonous HSD1 in moss and ferns appeared closest to the outgroup, V. carteri HSD-like, M. musculus HSD1, and H. sapiens HSD1. These data support the hypothesis that HSD1 originated in bryophytes and then in non-vascular and vascular plants, followed by HSD5 only in land plants. Gene structure analysis suggests that HSDs in plant species came up with a fixed number of six exons, and the intron phase was primarily 0, 1, 0, 0, and 0. Similarly, duplication analysis revealed that segmental duplications were the main reason for HSDs in plant species. Physicochemical properties suggest that dicotyledonous HSD1s and HSD5s were mainly acidic. The monocotyledonous HSD1s and HSD2s and the dicotyledonous HSD2s, HSD3s, HSD4s, and HSD6s were mainly basic, implying that HSDs in plants may have a variety of functions. Cis-regulatory elements and expression analysis revealed that HSDs in plants might have roles in several abiotic stresses. Due to the high expression of HSD1s and HSD5s in seeds, these HSDs in plants may have roles in fatty acid accumulation and degradation.
Nutrient stoichiometric and resorption characteristics of the petals of four common urban greening Rosaceae tree species
Authors: Song Dan , Liu Shuting , Fan Lide , Yang Jinyan , Li Haifang , Xia Yujie , Li Yuwu
Published: June 2023

Objective

Nutrient resorption efficiency and stoichiometric ratios are important strategies for understanding plants. The present study examined whether or not the nutrient resorption process of plant petals is similar to that of leaves and other vegetative organs, as well as the nutrient restriction status of the whole flowering process of plants in urban ecosystems.

Methods

Four Rosaceae tree species, Prunus yedoensis Matsum, Prunus serrulata var. lannesiana, Malus micromalus Makino, and Prunus cerasifera ‘Atropurpurea’, were selected as urban greening species to analyze the contents of C, N, P, and K elements in the petals and their stoichiometric ratios and nutrient resorption efficiencies.

Results

The results show interspecific differences in nutrient contents, stoichiometric ratios, and nutrient resorption efficiency of the fresh petals and petal litter of the four Rosaceae species. The nutrient resorption process was similar to that of the leaves before the petals fell. The nutrient contents of petals were higher than that of leaves at the global level, but the stoichiometric ratio and nutrient resorption efficiency of petals were lower. According to the "relative resorption hypothesis", N was limiting during the entire flowering period. The nutrient resorption efficiency of petals was positively correlated with nutrient variation. The correlation between the nutrient resorption efficiency of petals with nutrient content and stoichiometric ratio of petal litter was stronger.

Conclusion

The experimental results provide scientific basis and theoretical support for the selection, scientific maintenance and fertilization management of Rosaceae tree species in urban greening.
Family cohesion and adaptability reduces mobile phone addiction: the mediating and moderating roles of automatic thoughts and peer attachment
Authors: Lian Shuai-Lei , Cao Xiao-Xuan , Xiao Qing-Lu , Zhu Xiao-Wei , Yang Chen , Liu Qing-Qi
Published: June 2023

With the popularity of mobile Internet devices, the incidence of mobile phone addiction has been increasing, which has aroused the concern of all sectors of society. Due to the difficulty of eliminating the risk factors of mobile phone addiction, it’s significant for researchers to examine the function and underlying mechanisms of positive environmental factors in reducing individuals’ mobile phone addiction. Thus, the current study aimed to examine the relationship between family cohesion and adaptability and mobile phone addiction among university students and analyzed the mediating role of automatic thoughts as well as the moderating role of peer attachment in this link. The sample consisted of 958 Chinese university students. Participants completed self-report questionnaires assessing family cohesion and adaptability, mobile phone addiction, automatic thoughts, and peer attachment. PROCESS model 8 was significant (the total effect model (F (5, 952) = 19.64, R2 = 0.09, p < 0.001)). Results indicated that family cohesion and adaptability could not only negatively predict mobile phone addiction directly, but also indirectly through the mediating effect of automatic thoughts. Moreover, both the direct association between family cohesion and adaptability and mobile phone addiction as well as the indirect effect of automatic thoughts were moderated by peer attachment. Findings emphasized the beneficial role of peer attachment on the effect of family cohesion and adaptability on automatic thoughts and mobile phone addiction.
Shooter ready? Integrating mental skills training in an advanced sniper course
Authors: Ytterbøl Christian , Collins Dave , MacPherson Alan
Published: June 2023

Performance psychology has increased in usage and popularity; however, we contend that within the elite spheres of the military, there is a need for research and development to fit the context and environment. In this study, we describe an explorative case study on the integration of mental skill training techniques to an advanced sniper course in the Norwegian Armed Forces. We evaluate the impact through triangulation and examine results on the course, perceptions of the participants, and observations from the instructors. In addition, we conducted a 1-year follow-up to get participants' experience of translating the skills beyond the course. The results show that the mental skill training package influenced both results and performance in a positive manner; however, as a novel field, further research is warranted to establish a best practice to enhance performance for elite military forces.
Automatic depression severity assessment with deep learning using parameter-efficient tuning
Authors: Lau Clinton , Zhu Xiaodan , Chan Wai-Yip
Published: June 2023

Introduction

To assist mental health care providers with the assessment of depression, research to develop a standardized, accessible, and non-invasive technique has garnered considerable attention. Our study focuses on the application of deep learning models for automatic assessment of depression severity based on clinical interview transcriptions. Despite the recent success of deep learning, the lack of large-scale high-quality datasets is a major performance bottleneck for many mental health applications.

Methods

A novel approach is proposed to address the data scarcity problem for depression assessment. It leverages both pretrained large language models and parameter-efficient tuning techniques. The approach is built upon adapting a small set of tunable parameters, known as prefix vectors, to guide a pretrained model towards predicting the Patient Health Questionnaire (PHQ)-8 score of a person. Experiments were conducted on the Distress Analysis Interview Corpus - Wizard of Oz (DAIC-WOZ) benchmark dataset with 189 subjects, partitioned into training, development, and test sets. Model learning was done on the training set. Prediction performance mean and standard deviation of each model, with five randomly-initialized runs, were reported on the development set. Finally, optimized models were evaluated on the test set.

Results

The proposed model with prefix vectors outperformed all previously published methods, including models which utilized multiple types of data modalities, and achieved the best reported performance on the test set of DAIC-WOZ with a root mean square error of 4.67 and a mean absolute error of 3.80 on the PHQ-8 scale. Compared to conventionally fine-tuned baseline models, prefix-enhanced models were less prone to overfitting by using far fewer training parameters (<6% relatively).

Discussion

While transfer learning through pretrained large language models can provide a good starting point for downstream learning, prefix vectors can further adapt the pretrained models effectively to the depression assessment task by only adjusting a small number of parameters. The improvement is in part due to the fine-grain flexibility of prefix vector size in adjusting the model's learning capacity. Our results provide evidence that prefix-tuning can be a useful approach in developing tools for automatic depression assessment.
Association between war-related traumatic events and blood pressure trajectory: a population-based study among the mid-aged and older Palestinian adults living in Gaza
Authors: Jebril Majed , Mazidi Mohsen , Liu Xin , Baibing Mi , Arafat Heba , Shi Zumin , Wang Youfa
Published: June 2023

Background

Little is known regarding health status in an environment characterized by instability and ongoing war risks. This study investigated hypertension disease burden and associations of war-related traumatic events with blood pressure (BP) trajectory over time amongst mid-aged and older Palestinian adults in Gaza Strip.

Methods

From nine primary healthcare centers, medical records between 2013 and 2019 were collected for 1,000 mid-aged and older Palestinian adults living in Gaza. Multinomial logistic regression analysis examined associations between war-related traumatic events and BP trajectories derived using latent class trajectory analysis (LCTA).

Results

The prevalence of self-reported injury (of participants or their family members), death of a family member, and violence due to house bombing was 51.4%, 54.1%, and 66.5%, respectively. In total, 22.4% and 21.4% of participants had constant-very-high (CVH) systolic BP (SBP) (>160 mmHg) and diastolic BP (DBP) (>95 mmHg), and normal-stable SBP and DBP was found only 54.9% and 52.6%, respectively. Injury (participants or family members), death of a family member, and violence due to house bombing during wars were associated with CVH SBP with odds ratios [95 CI, OR = 1.79 (1.28–2.48), 1.90 (1.36–2.65), and 1.44 (1.01–2.05)], respectively. The corresponding figures were [95 CI, OR = 1.92 (1.36–2.71), 1.90 (1.35–2.68), and 1.62 (1.13–2.38)] for CVH DBP. Living in debt was positively associated with CVH SBP, [95 CI, OR = 2.49 (1.73–3.60)] and CVH DBP, [95 CI, OR = 2.37 (1.63–3.45)].

Conclusion

The disease burden related to war-related traumatic events is high and positively related to adverse BP trajectory among the mid-aged and older Palestinians living in Gaza. Intervention programs are needed to manage and prevent chronic diseases in this vulnerable population.
Knowledge, attitudes, and practices about COVID-19 pandemic: a bibliometric analysis
Authors: Selva-Pareja Laia , Camí Carla , Roca Judith , Espart Anna , Campoy Carme , Botigué Teresa
Published: June 2023

Background

In order to avoid high rates of COVID-19 infection, one of the main tasks that must be performed is to improve the knowledge, attitudes, and practices (KAP) about the virus. In this sense, Health Education is an essential tool for dealing with the virus. The aim of health education is to educate individuals through educational, motivational, skill development, and awareness techniques, and an understanding of the main needs of KAP is essential for this. Many KAP studies were published during the COVID-19 pandemic, and the aim of the present study was to analyze these publications through a bibliometric study.

Methods

A bibliometric analysis of the publications on KAP and COVID-19 was conducted in the Web of Science Core Collection database. The RStudio Bibliometrix and VOSviewer packages were utilized to analyze the scientific production, authors, citations, countries, publishers, journals, research areas, and keywords.

Results

Of the 1,129 articles published, 777 were included in the study. The year with the most publications and citations was 2021. Three authors were underlined (all from Ethiopia), due to the number of articles published, the number of citations, and the collaboration networks established. As for the countries, most of the publications came from Saudi Arabia, while China obtained the most citations. PLOS One and Frontiers in Public Health published the most articles on the subject. The most frequent keywords were knowledge, attitudes, practices, and COVID-19. At the same time, others were identified based on the population group analyzed.

Conclusion

This is the first bibliometric study on KAP and COVID-19. The significant number of publications identified on KAP and its relationship to the COVID-19 pandemic, in the span of only 3 years, indicates the increased interest in this area. The study provides relevant information to researchers who are approaching this subject for the first time. It is a useful tool that can stimulate new studies and collaborations between researchers from different countries, areas and approaches. At the methodological level, a step-by-step guide is provided for future authors who wish to perform a bibliometric analysis.