bluuebunny/crossref_metadata_2025 huggingface dataset:
Gabapentin-Associated Movement Disorders: A Literature Review
Authors: Rissardo Jamir Pitton , Medeiros Araujo de Matos Ursula , Fornari Caprara Ana Letícia
Published: September 2023

Background: Gabapentin (GBP)-induced movement disorders (MDs) are under-recognized adverse drug reactions. They are commonly not discussed with patients, and their sudden occurrence can lead to misdiagnosis. This literature review aims to evaluate the clinical–epidemiological profile, pathological mechanisms, and management of GBP-associated MD. Methods: Two reviewers identified and assessed relevant reports in six databases without language restriction between 1990 and 2023. Results: A total of 99 reports of 204 individuals who developed a MD associated with GBP were identified. The MDs encountered were 135 myoclonus, 22 dyskinesias, 7 dystonia, 3 akathisia, 3 stutterings, 1 myokymia, and 1 parkinsonism. The mean and median ages were 54.54 (SD: 17.79) and 57 years (age range: 10–89), respectively. Subjects were predominantly male (53.57%). The mean and median doses of GBP when the MD occurred were 1324.66 (SD: 1117.66) and 1033 mg/daily (GBP dose range: 100–9600), respectively. The mean time from GBP-onset to GBP-associated MD was 4.58 weeks (SD: 8.08). The mean recovery time after MD treatment was 4.17 days (SD: 4.87). The MD management involved GBP discontinuation. A total of 82.5% of the individuals had a full recovery in the follow-up period. Conclusions: Myoclonus (GRADE A) and dyskinesia (GRADE C) were the most common movement disorders associated with GBP.
Amidoxime Modified UiO-66@PIM-1 Mixed-Matrix Membranes to Enhance CO2 Separation and Anti-Aging Performance
Authors: Gao Jiaming , Sun Yongchao , Kang Feifei , Guo Fei , He Gaohong , Wang Hanli , Yang Zhendong , Ma Canghai , Jiang Xiaobin , Xiao Wu
Published: September 2023

Mixed matrix membranes (MMMs) generally have some fatal defects, such as poor compatibility between the two phases leading to non-selective pores. In this work, PIM-1 was chosen as the polymer matrix, and UiO-66 modified with amidoxime (UiO-66-AO) was used as the filler to prepare the MMMs. In the MMMs, the amino and hydroxyl groups on UO-66-AO form a rich hydrogen bond network with the N and O atoms in the polymer PIM-1 chain to improve the compatibility between the polymer matrix and the filler. In addition, the selective adsorption of CO2 by the amidoxime group can promote the transport of CO2 in the membrane, which enhances the gas selectivity. The CO2 permeability and CO2/N2 selectivity of UiO-66-AO@PIM-1 MMMs are increased by 35.2% and 45.2% compared to pure PIM-1 membranes, reaching 7535.5 Barrer and 26.9, surpassing the Robeson Upper Bound (2008) and close to the 2019 Upper Bound. After 38 days of the aging experiment, the CO2 permeability is approximately 74% of the original. The results show that the addition of UiO-66-AO has an obvious effect on improving the aging properties of the membrane. The UiO-66-AO@PIM-1 MMMs have a bright prospect for CO2 separation in the future.
Effect of Al5TiB Master Alloy with P on Microstructure and Mechanical Properties of AlSi7Mg Alloy
Authors: Lipiński Tomasz
Published: September 2023

Aluminum-silicon alloys are popular casting alloys. In its raw state, the microstructure of the hypoeutectic silumin consists of a large eutectic β phase against the background of dendritic eutectic α. Due to its large microstructure components, mainly the eutectic β phase, this alloy has low mechanical properties. The unfavorable properties of hypoeutectic silumin can be improved by changing the size and shape of the alloy’s microstructure components. There are several possibilities for controlling the microstructure and the resulting mechanical properties of the alloy. One possibility is to modify the alloy with elements and chemical compounds. This paper presents the effect of phosphorus with Al-Ti-B on the microstructure and mechanical properties of hypoeutectic silumin AlSi7Mg. The proportions of Ti to B were selected on the basis of the results presented in the literature, recognizing the optimal ratio of 5:1. The modifier was introduced into the alloy in the form of an AlTiBP master alloy with a variable content of titanium, boron, and phosphorus. Phosphorus was added at the levels of 0.1, 0.2, and 0.3% of the weight of the modified casting. As a result of the tests carried out, the modifying effect of the introduced master alloy was confirmed. A different morphology of microstructures was obtained for the different chemical compositions of the modifier. The most favorable modification effect, whose measurable parameter is the highest (out of the obtained) mechanical properties, was found for the modifier containing 0.25% Ti + 0.03% B + 0.2% P. It was also found that phosphorus, in the presence of titanium and boron, affects the microstructure and mechanical properties of hypoeutectic silumin AlSi7Mg.
Study on Reactive Air Brazing of p-SiO2 Ceramic with Ag-xCuO Filler Metal
Authors: Chen Yongwei , Ma Qiang , He Peng
Published: September 2023

Reactive air brazing of porous SiO2 ceramic (p-SiO2) was achieved using Ag-CuO filler metal. When brazing p-SiO2, two main problems existed. Firstly, the wettability of the Ag filler metal on the surface of p-SiO2 was poor. Secondly, the residual stress caused by the mismatch of the coefficient of thermal expansion was high in the joint. In order to solve these problems, the effects of CuO contents on the p-SiO2 brazed joint were analyzed. In a wetting experiment, the addition of CuO significantly improved the wettability of the Ag-CuO/p-SiO2 system. With the content of CuO increasing, the contact angle decreased from 90° to 0°. In addition, when the content of CuO increased to 0.5 mol%, the contact angle decreased from 90° to 52°. Then, during brazing p-SiO2 with the Ag-xCuO filler metal, the typical interfacial microstructure of the joints brazed at 1000 °C for 30 min was p-SiO2 ceramic/Ag (s,s) + SiO2 + CuO/Ag (s,s)/Ag (s,s) + SiO2 + CuO/p-SiO2 ceramic. Meanwhile, Ag-CuO infiltrated into the p-SiO2 ceramic and an infiltration layer formed. The infiltration layer was composed of Ag (s,s) + SiO2 + CuO and the infiltration layer was conductive to form a good gradient transition of the coefficient of thermal expansion (CTE). Then, the residual stress in the joint was released and the shear strength improved. In addition, with the content of CuO increasing, the depth of the infiltration layer increased. Furthermore, when the content of CuO was 0.5 mol%, the maximum shear strength of the joint was 55 MPa.
Study on the Tensile and Fatigue Properties of the FH36 Ship Steel Plates at Room and Low Temperatures
Authors: Wang Dong , Yan Ling , Yin Wei , Zhang Peng , Wang Zhenmin , Li Guanglong , Hu Xiaodong , Li Boyong , Zhang Wanshun , Zhu Jing
Published: September 2023

This study investigated the tensile properties and fatigue behavior of FH36 steel plates subjected to alternating rolling processes in different directions, as well as their performance at room temperature and −60 °C. The results revealed that by employing sequential rolling in both the rolling and transverse directions, the disparities in the mechanical properties between these two directions were eliminated, resulting in nearly identical tensile performances. The macroscopic features of the high-cycle and low-cycle fatigue fractures at both room temperature and at −60 °C were similar, with high-cycle fatigue fractures exhibiting oblique shear features and low-cycle fatigue fractures exhibiting cup-cone shapes. The crack initiation zones were consistently located on the surface of the specimens. As the maximum stress increased, the area of the high-cycle fatigue crack propagation zone decreased, while the area of the final fracture zone, the number of dimples, and the proportion of low-angle grain boundaries all increased. Under −60 °C conditions, the critical crack length for high-cycle fatigue, the maximum stress limit for the onset of high-cycle fatigue, and the fatigue limit were all higher than those at room temperature, indicating a superior low-temperature fatigue performance.
Effects of Heated Drinking Water during the Cold Season on Serum Biochemistry, Ruminal Fermentation, Bacterial Community, and Metabolome of Beef Cattle
Authors: He Tengfei , Yi Guang , Wang Xilin , Sun Yan , Li Jiangong , Wu Zhenlong , Guo Yao , Sun Fang , Chen Zhaohui
Published: September 2023

This study explored the effects of drinking heated water in the cold seasons on the serum metabolism, rumen microbial fermentation, and metabolome of beef cattle. Twelve fattening cattle (642 ± 14.6 kg) aged 21 to 22 months were randomly and equally divided into two groups based on body weight: one receiving room-temperature water (RTW; average 4.39 ± 2.55 °C) and the other heated water (HW; average 26.3 ± 1.70 °C). The HW group displayed a significant decrease in serum glucose (p < 0.01) and non-esterified fatty acid (p < 0.01), but increases in insulin (p = 0.04) and high-density lipoprotein (p = 0.03). The rumen fermentation parameters of the HW group showed substantial elevations in acetate (p = 0.04), propionate (p < 0.01), isobutyrate (p = 0.02), and total volatile fatty acids (p < 0.01). Distinct bacterial composition differences were found between RTW and HW at the operational taxonomic unit (OTU) level (R = 0.20, p = 0.01). Compared to RTW, the HW mainly had a higher relative abundance of Firmicutes (p = 0.07) at the phylum level and had a lower abundance of Prevotella (p < 0.01), norank_f_p-215-o5 (p = 0.03), and a higher abundance of NK4A214_group (p = 0.01) and Lachnospiraceae_NK3A20_group (p = 0.05) at the genus level. In addition, NK4A214_group and Lachnospiraceae_NK3A20_group were significantly positively correlated with the rumen propionate and isovalerate (r > 0.63, p < 0.05). Prevotella was negatively correlated with rumen propionate and total volatile fatty acids (r = −0.61, p < 0.05). In terms of the main differential metabolites, compared to the RTW group, the expression of Cynaroside A, N-acetyl-L-glutamic acid, N-acetyl-L-glutamate-5-semialdehyde, and Pantothenic acid was significantly upregulated in HW. The differentially regulated metabolic pathways were primarily enriched in nitrogen metabolism, arginine biosynthesis, and linoleic acid metabolism. Prevotella was significantly positively correlated with suberic acid and [6]-Gingerdiol 3,5-diacetate (r > 0.59, p < 0.05) and was negatively correlated with Pantothenic acid and isoleucyl-aspartate (r < −0.65, p < 0.05). NK4A214_group was positively correlated with L-Methionine and glycylproline (r > 0.57, p < 0.05). Overall, our research demonstrates the important relationship between drinking water temperature and metabolic and physiological responses in beef cattle. Heating drinking water during cold seasons plays a pivotal role in modulating internal energy processes. These findings underscore the potential benefits of using heated water as a strategic approach to optimize energy utilization in beef cattle during the cold seasons.
Femtosecond Laser Direct Writing of Gecko-Inspired Switchable Adhesion Interfaces on a Flexible Substrate
Authors: Zhang Zhiang , He Bingze , Han Qingqing , He Ruokun , Ding Yuxuan , Han Bing , Ma Zhuo-Chen
Published: September 2023

Biomimetic switchable adhesion interfaces (BSAIs) with dynamic adhesion states have demonstrated significant advantages in micro-manipulation and bio-detection. Among them, gecko-inspired adhesives have garnered considerable attention due to their exceptional adaptability to extreme environments. However, their high adhesion strength poses challenges in achieving flexible control. Herein, we propose an elegant and efficient approach by fabricating three-dimensional mushroom-shaped polydimethylsiloxane (PDMS) micropillars on a flexible PDMS substrate to mimic the bending and stretching of gecko footpads. The fabrication process that employs two-photon polymerization ensures high spatial resolution, resulting in micropillars with exquisite structures and ultra-smooth surfaces, even for tip/stem ratios exceeding 2 (a critical factor for maintaining adhesion strength). Furthermore, these adhesive structures display outstanding resilience, enduring 175% deformation and severe bending without collapse, ascribing to the excellent compatibility of the micropillar’s composition and physical properties with the substrate. Our BSAIs can achieve highly controllable adhesion force and rapid manipulation of liquid droplets through mechanical bending and stretching of the PDMS substrate. By adjusting the spacing between the micropillars, precise control of adhesion strength is achieved. These intriguing properties make them promising candidates for various applications in the fields of microfluidics, micro-assembly, flexible electronics, and beyond.
Paragenesis and Formation Mechanism of the Dolomite-Mottled Limestone Reservoir of Ordovician Ma4 Member, Ordos Basin
Authors: Yang Zeguang , Wang Aiguo , Fan Liyong , Ma Zhanrong , Luo Xiaorong , Ning Xinghui , Meng Kun
Published: September 2023

Despite the discovery of high-producing natural gas reservoirs in the low-permeability dolomite-mottled limestone (DML) reservoir of the fourth Member (Ma4) of the Majiagou Formation in the Ordos Basin, the current understanding of the processes responsible for reservoir formation are still superficial, which extremely restricts the effectiveness of deep petroleum exploration and development in the basin. Therefore, this study analyzed the paragenesis process of the DML reservoir through systematic petrographic and geochemical measurements. The DML consists of burrows and matrix. The burrows are mainly filled with dolomite with a small amount of micrite, calcite cement, and solid bitumen. The matrix mainly consists of wakestone or mudstone. The DML has experienced multiple diagenetic events, including seepage-reflux dolomitization, compaction, calcite cement CaI cementation, micrite recrystallization, dissolution, hydrocarbon charging, calcite cement CaII cementation, and dolomite progressive recrystallization. Dolomitization is critical to the DML reservoir formation. The pore created by dolomitization is the hydrocarbon-migrated pathway and storage space. Due to the difference in Mg2+-rich fluid supply, the degree of dolomitization decreases from west to east, which causes the difference in diagenetic evolution of the western and eastern parts of the study area. The high dolomitization degree led to strong anti-compaction ability in the west, contrary to the east. Thus, the reservoir quality of the west is better than the east.
Earth Abundant Oxidation Catalysts for Removal of Contaminants of Emerging Concern from Wastewater: Homogeneous Catalytic Screening of Monomeric Complexes
Authors: Garcia Leslie , Koper Makynna R. , Mondal Somrita , Priddle Joshua T. , Truong William A. , Allbritton Elisabeth M. A. , McAdoo Ashtyn G. , Cannon-Smith Desiray J. , Funwie Neil L. , Hoang Tuyet , Kim Inseo , Hubin David J. , Krause Jeanette A. , Oliver Allen G. , Prior Timothy J. , Hubin Timothy J.
Published: September 2023

Twenty novel Mn, Fe, and Cu complexes of ethylene cross-bridged tetraazamacrocycles with potentially copolymerizable allyl and benzyl pendant arms were synthesized and characterized. Multiple X-ray crystal structures demonstrate the cis-folded pseudo-octahedral geometry forced by the rigidifying ethylene cross-bridge and show that two cis coordination cites are available for interaction with substrate and oxidant. The Cu complexes were used to determine kinetic stability under harsh acidic and high-temperature conditions, which revealed that the cyclam-based ligands provide superior stabilization with half-lives of many minutes or even hours in 5 M HCl at 50–90 °C. Cyclic voltammetry studies of the Fe and Mn complexes reveal reversible redox processes indicating stabilization of Fe2+/Fe3+ and Mn2+/Mn3+/Mn4+ oxidation states, indicating the likelihood of catalytic oxidation for these complexes. Finally, dye-bleaching experiments with methylene blue, methyl orange, and rhodamine B demonstrate efficient catalytic decolorization and allow selection of the most successful monomeric catalysts for copolymerization to produce future heterogeneous water purification materials.
Grape-Derived Polysaccharide Extracts Rich in Rhamnogalacturonans-II as Potential Modulators of White Wine Flavor Compounds
Authors: Canalejo Diego , Martínez-Lapuente Leticia , Ayestarán Belén , Pérez-Magariño Silvia , Doco Thierry , Guadalupe Zenaida
Published: September 2023

Many authors have investigated the role of mannoproteins on wine quality, but very few have analyzed the use of grape-derived polysaccharides as they are not commercially available. In this study, purified grape-derived polysaccharides from red wine (WPP) and winemaking by-products (DWRP: Distilled Washing Residues Polysaccharides) were used as potential fining agents to modulate white wine flavor. Phenolics and volatile compounds were analyzed in the control and wines treated with WPP, DWRP, and commercial mannoproteins (CMs) after one and twelve months of bottling, and a sensory analysis was conducted. WPP and DWRP, rich in rhamnogalacturonans-II, showed themselves to be good modulators of wine aroma and astringency. Improvement in wine aroma was related to an increase in all volatile families expect higher alcohols and volatile acids. The modulation of astringency and bitterness was related to a reduction in the proanthocyanidin content and its mean degree of polymerization. Extracts with polysaccharides with higher protein contents presented a higher retention of volatile compounds, and DWRP extract had more positive effects on the overall aroma. Our novel results present the possibility of obtaining valuable polysaccharides from distilled washing residues of wine pomaces, which could promote its valorization as a by-product. This is the first time the potential use of this by-product has been described.
Increasing the Consumption of Environmentally Friendly Foods in a University Dining Hall Using Menu Item Placement
Authors: Franchini Cinzia , Bartolotto Carole , Scazzina Francesca , Carpenter Catherine L. , Slusser Wendelin
Published: September 2023

Creating a decision-making environment that promotes sustainable food choices is a priority for both the individual and society. This study aimed at encouraging plant-based menu choices by re-ordering the menu according to the carbon footprint values. The project was conducted in a grab-and-go eatery at a large United States public university, where students could order their meals choosing among different menu options that were customizable with various ingredients. The order of menu ingredients was changed twice: for five weeks, from the most to the least impactful in terms of carbon footprint; subsequently, for another five weeks the order was reversed. At both times, all sales data were recorded. A total of 279,219 and 288,527 items were selected, respectively, during the first and the second intervention. A significant association was found between menu re-ordering and customers’ choices for almost all food categories considered. Overall, despite beef choices not changing, results showed that students were more likely to choose low-carbon options when these were placed at the beginning, emphasizing that food selections were impacted by ingredient placement on the menu list. These findings highlight the need for a multi-level strategy focused on raising students’ awareness of the environmental impact of animal-based foods, particularly beef.
Association between Maternal Body Composition in Second Trimester and Risk of Fetal Macrosomia: A Population-Based Retrospective Study in China
Authors: He Yirong , Huang Chuanya , Luo Biru , Liao Shujuan
Published: September 2023

(1) Background: Female body composition undergoes significant changes to support fetal growth and development during pregnancy. This study investigated the association of maternal body composition in the second trimester and macrosomia and explored whether body-composition-related indicators could be used to predict macrosomia. (2) Methods: This study was conducted in China from December 2016 to December 2021. Women with singleton pregnancies, gestational ages between 37 and 42 weeks, and an absence of pregnancy complications were included. In the second trimester, bioelectric impedance analysis (BIA) was used to measure body-composition-related indicators. Logistic regression analysis was performed to explore the risk factors for macrosomia. The predictive performance of maternal body composition and clinical indicators for macrosomia were assessed using the area under the receiver-operating-characteristics curve (AUC). (3) Results: This retrospective study involved 43,020 pregnant women; we collected 2008 cases of macrosomia. Gravidity, gestational age, body mass index (BMI), gestational weight gain (GWG), total body water, fat mass, fat-free mass (FFM), skeletal muscle mass, and visceral fat level were risk factors for macrosomia (p < 0.05 for all). In the prediction model, the AUC of FFM for predicting macrosomia was the largest (0.742). (4) Conclusions: Body-composition-related indicators associated with macrosomia and body composition measurements in the second trimester can predict the risk of macrosomia, enabling clinicians to implement interventions earlier to reduce adverse perinatal outcomes.
Non-Digestible Carbohydrates: Green Extraction from Food By-Products and Assessment of Their Effect on Microbiota Modulation
Authors: Expósito-Almellón Xavier , Duque-Soto Carmen , López-Salas Lucía , Quirantes-Piné Rosa , de Menezes Cristiano Ragagnin , Borrás-Linares Isabel , Lozano-Sánchez Jesús
Published: September 2023

The nature and composition of the waste produced by food industrial processing make its abundance and accumulation an environmental problem. Since these by-products may present a high potential for revalorization and may be used to obtain added-value compounds, the main goals of the technological advancements have been targeted at reducing the environmental impact and benefiting from the retrieval of active compounds with technological and health properties. Among the added-value substances, nondigestible carbohydrates have demonstrated promise. In addition to their well-known technological properties, they have been discovered to modify the gut microbiota and enhance immune function, including the stimulation of immune cells and the control of inflammatory reactions. Furthermore, the combination of these compounds with other substances such us phenols could improve their biological effect on different noncommunicable diseases through microbiota modulation. In order to gain insight into the implementation of this combined strategy, a broader focus concerning different aspects is needed. This review is focused on the optimized green and advanced extraction system applied to obtain added-value nondigestible carbohydrates, the combined administration with phenols and their beneficial effects on microbiota modulation intended for health and/or illness prevention, with particular emphasis on noncommunicable diseases. The isolation of nondigestible carbohydrates from by-products as well as in combination with other bioactive substances could provide an affordable and sustainable source of immunomodulatory chemicals.
Stand Up to Stand Out: Natural Dietary Polyphenols Curcumin, Resveratrol, and Gossypol as Potential Therapeutic Candidates against Severe Acute Respiratory Syndrome Coronavirus 2 Infection
Authors: Wang Zhonglei , Song Xian-qing , Xu Wenjing , Lei Shizeng , Zhang Hao , Yang Liyan
Published: September 2023

The COVID-19 pandemic has stimulated collaborative drug discovery efforts in academia and the industry with the aim of developing therapies and vaccines that target SARS-CoV-2. Several novel therapies have been approved and deployed in the last three years. However, their clinical application has revealed limitations due to the rapid emergence of viral variants. Therefore, the development of next-generation SARS-CoV-2 therapeutic agents with a high potency and safety profile remains a high priority for global health. Increasing awareness of the “back to nature” approach for improving human health has prompted renewed interest in natural products, especially dietary polyphenols, as an additional therapeutic strategy to treat SARS-CoV-2 patients, owing to its good safety profile, exceptional nutritional value, health-promoting benefits (including potential antiviral properties), affordability, and availability. Herein, we describe the biological properties and pleiotropic molecular mechanisms of dietary polyphenols curcumin, resveratrol, and gossypol as inhibitors against SARS-CoV-2 and its variants as observed in in vitro and in vivo studies. Based on the advantages and disadvantages of dietary polyphenols and to obtain maximal benefits, several strategies such as nanotechnology (e.g., curcumin-incorporated nanofibrous membranes with antibacterial-antiviral ability), lead optimization (e.g., a methylated analog of curcumin), combination therapies (e.g., a specific combination of plant extracts and micronutrients), and broad-spectrum activities (e.g., gossypol broadly inhibits coronaviruses) have also been emphasized as positive factors in the facilitation of anti-SARS-CoV-2 drug development to support effective long-term pandemic management and control.
Bone Health, Intersectionality and Climate Change
Authors: Dennison Elaine
Published: September 2023

Extreme weather patterns are becoming more common, with attendant risks for human health [...]
Characterization of Viral Interference in Aedes albopictus C6/36 Cells Persistently Infected with Dengue Virus 2
Authors: González-Flores Aurora Montsserrat , Salas-Benito Mariana , Rosales-García Victor Hugo , Zárate-Segura Paola Berenice , Del Ángel Rosa María , De Nova-Ocampo Mónica Ascención , Salas-Benito Juan Santiago
Published: September 2023

Arboviruses are an important group of pathogens that cause diseases of medical and veterinary concern worldwide. The interactions of these viruses with their host cells are complex, and frequently, the coexistence of two different viruses in the same cell results in the inhibition of replication in one of the viruses, which is a phenomenon called viral interference. This phenomenon can be exploited to develop antiviral strategies. Insect cell lines persistently infected with arboviruses are useful models with which to study viral interference. In this work, a model of C6/36-HT cells (from Aedes albopictus mosquitoes) persistently infected with Dengue virus, serotype 2, was used. Viral interference was evaluated via plaque and flow cytometry assays. The presence of heterotypic interference against the other serotypes of the same virus and homologous interference against yellow fever virus was determined; however, this cell line did not display heterologous viral interference against Sindbis virus. The mechanisms responsible for viral interference have not been fully elucidated, but small RNAs could be involved. However, the silencing of Ago3, a key protein in the genome-derived P-element-induced wimpy testis pathway, did not alter the viral interference process, suggesting that viral interference occurs independent of this pathway.
Antibacterial and Anti-Biofilm Activities of Cinnamon Oil against Multidrug-Resistant Klebsiella pneumoniae Isolated from Pneumonic Sheep and Goats
Authors: Mahrous Sara H. , El-Balkemy Farouk A. , Abo-Zeid Naser Z. , El-Mekkawy Mamdouh F. , El Damaty Hend M. , Elsohaby Ibrahim
Published: September 2023

The primary objectives were to isolate and identify Klebsiella pneumoniae (K. pneumoniae), and determine the antimicrobial resistance patterns and biofilm formation abilities of the isolates. Additionally, the study aimed to investigate the antimicrobial and anti-biofilm effects of cinnamon oil against K. pneumoniae isolates. A cross-sectional study was conducted from March 2022 to April 2023 to collect 200 samples (including 156 nasal swabs and 44 lung specimens) from pneumonic sheep and goats admitted to the Veterinary Teaching Hospital of Zagazig University, Egypt. K. pneumoniae was isolated from a total of 72 (36%) samples, with 53 (73.6%) isolates recovered from nasal swabs and 19 (26.4%) from lung samples. Among the samples, 52 (36.9%) were from sheep and 20 (33.9%) were from goats. Antimicrobial susceptibility testing of the 72 K. pneumoniae isolates to 18 antimicrobials revealed that all isolates were resistant to ampicillin, amoxicillin/clavulanic acid, cefotaxime, ceftriaxone, tetracycline, colistin, fosfomycin, and trimethoprim/sulphamethoxazole. None of the isolates were resistant to amikacin, imipenem, and norfloxacin. Multidrug resistance (MDR) was observed in all K. pneumoniae isolates recovered from sheep and goats. The average MAR index was 0.71, ranging from 0.50 to 0.83. Regarding biofilm formation, among the K. pneumoniae isolates with a high MAR index (n = 30), 10% exhibited strong formation, 40% showed moderate formation, 43.3% displayed weak formation, and 6.7% did not form biofilms. Additionally, the biofilm-forming genes treC and fimA were present in all 28 biofilm-forming K. pneumoniae isolates, while the mrkA gene was detected in 15 (53.6%) of the 28 isolates. MDR K. pneumoniae isolates with strong biofilm formation abilities were treated with cinnamon oil at varying concentrations (100%, 75%, 50%, and 25%). This treatment resulted in inhibition zone diameters ranging from 35 to 45 mm. Cinnamon oil exhibited lower minimum inhibitory concentration and minimum bactericidal concentration values compared to norfloxacin for all isolates. Additionally, cinnamon oil significantly reduced the expression of biofilm-associated genes (treC, fimA, and mrkA) when compared to isolates treated with norfloxacin or untreated. In conclusion, this study identified a high level of MDR K. pneumoniae with strong and moderate biofilm formation abilities in pneumonic sheep and goats in Sharika Governorate, Egypt. Although cinnamon oil demonstrated potential antibacterial and anti-biofilm properties against K. pneumoniae, further research is required to investigate its effectiveness in treating K. pneumoniae infections in pneumonic sheep and goats.
The Development of Magnolol-Loaded Intravenous Emulsion with Low Hepatotoxic Potential
Authors: Gostyńska Aleksandra , Czerniel Joanna , Kuźmińska Joanna , Żółnowska Izabela , Brzozowski Jakub , Krajka-Kuźniak Violetta , Stawny Maciej
Published: September 2023

Intestinal failure-associated liver disease (IFALD) is a severe liver injury occurring due to factors related to intestinal failure and parenteral nutrition administration. Different approaches are studied to reduce the risk or ameliorate the course of IFALD, including providing omega-3 fatty acids instead of soybean oil-based lipid emulsion or administering active compounds that exert a hepatoprotective effect. This study aimed to develop, optimize, and characterize magnolol-loaded intravenous lipid emulsion for parenteral nutrition. The preformulation studies allowed for chosen oils mixture of the highest capacity of magnolol solubilization. Then, magnolol-loaded SMOFlipid was developed using the passive incorporation method. The Box–Behnken design and response surface methodology were used to optimize the entrapment efficiency. The optimal formulation was subjected to short-term stress tests, and its effect on normal human liver cells and erythrocytes was determined using the MTT and hemolysis tests, respectively. The optimized magnolol-loaded SMOFlipid was characterized by the mean droplet diameter of 327.6 ± 2.9 nm with a polydispersity index of 0.12 ± 0.02 and zeta potential of −32.8 ± 1.2 mV. The entrapment efficiency of magnolol was above 98%, and pH and osmolality were sufficient for intravenous administration. The magnolol-loaded SMOFlipid samples showed a significantly lower toxic effect than bare SMOFlipid in the same concentration on THLE-2 cells, and revealed an acceptable hemolytic effect of 8.3%. The developed formulation was characterized by satisfactory stability. The in vitro studies showed the reduced cytotoxic effect of MAG-SMOF applied in high concentrations compared to bare SMOFlipid and the non-hemolytic effect on human blood cells. The magnolol-loaded SMOFlipid is promising for further development of hepatoprotective lipid emulsion for parenteral nutrition.
In Vitro and In Vivo Evaluation of Inhalable Ciprofloxacin Sustained Release Formulations
Authors: Shi Changzhi , Guo Kewei , Zhang Li , Guo Yi , Feng Yu , Cvijić Sandra , Cun Dongmei , Yang Mingshi
Published: September 2023

Respiratory antibiotics delivery has been appreciated for its high local concentration at the infection sites. Certain formulation strategies are required to improve pulmonary drug exposure and to achieve effective antimicrobial activity, especially for highly permeable antibiotics. This study aimed to investigate lung exposure to various inhalable ciprofloxacin (CIP) formulations with different drug release rates in a rat model. Four formulations were prepared, i.e., CIP-loaded PLGA micro-particles (CHPM), CIP microcrystalline dry powder (CMDP), CIP nanocrystalline dry powder (CNDP), and CIP spray-dried powder (CHDP), which served as a reference. The physicochemical properties, drug dissolution rate, and aerosolization performance of these powders were characterized in vitro. Pharmacokinetic profiles were evaluated in rats. All formulations were suitable for inhalation (mass median aerodynamic diameter < 5 µm). CIP in CHPM and CHDP was amorphous, whereas the drug in CMDP and CNDP remained predominantly crystalline. CHDP exhibited the fastest drug release rate, while CMDP and CNDP exhibited much slower drug release. In addition, CMDP and CNDP exhibited significantly higher in vivo lung exposure to CIP compared with CHDP and CHPM. This study suggests that lung exposure to inhaled drugs with high permeability is governed by drug release rate, implying that lung exposure of inhaled antibiotics could be improved by a sustained-release formulation strategy.
Functional Segmentation Method for the Design of Compact Adiabatic Devices
Authors: Liang Tu-Lu , Cheng Xi , Yu Mei , Shi Jin , Wu Gangxiong , Jiang Ruirui , Rong Weiwei , Shao Wei
Published: September 2023

In this study, a functional segmentation method for the design of adiabatic devices is presented. This method can be applied to calculate the compact adiabatic waveguide shapes for adiabatic taper waveguides and adiabatic mode converters. We introduce two algorithms, namely the quadratic function segmentation design for the adiabatic taper waveguide and the cubic function segmentation design for the adiabatic mode coupler. The design shows that for each segment, there is no need for separate simulations to obtain the length of each segment, and only the length of each segment can be determined by equations. This approach is much less cumbersome than other numerical design methods for adiabatic devices. The proposed functional segmentation design requires only a device length of 36 μm to achieve 95% power transfer efficiency, while a traditional linear-shape connection requires a device length of 54 μm to achieve the same power transfer efficiency. Therefore, the device designed by the function segmentation method has better compactness compared to the conventional linear-shape connection design of adiabatic devices.
Evaluation of Climate Change Impacts on the Potential Distribution of Wild Radish in East Asia
Authors: Han Qingxiang , Liu Ye , Jiang Hongsheng , Chen Xietian , Feng Huizhe
Published: September 2023

Climate change can exert a considerable influence on the geographic distribution of many taxa, including coastal plants and populations of some plant species closely related to those used as agricultural crops. East Asian wild radish, Raphanus raphanistrum subsp. sativus, is an annual coastal plant that is a wild relative of the cultivated radish (R. sativus). It has served as source of genetic material that has been helpful to develop and improve the quality and yield of radish crops. To assess the impact of climate change on wild radish in East Asia, we analyzed its distribution at different periods using the maximum entropy model (MaxEnt). The results indicated that the precipitation of the driest month (bio14) and precipitation seasonality (bio15) were the two most dominant environmental factors that affected the geographical distribution of wild radish in East Asia. The total potential area suitable for wild radish is 102.5574 × 104 km2, mainly located along the seacoasts of southern China, Korea, and the Japanese archipelago. Compared with its current distribution regions, the potentially suitable areas for wild radish in the 2070s will further increase and expand northwards in Japan, especially on the sand beach habitats of Hokkaido. This research reveals the spatiotemporal changes for the coastal plant wild radish under global warming and simultaneously provides a vital scientific basis for effective utilization and germplasm innovation for radish cultivars to achieve sustainable agriculture development.
Insecticidal Activity of Tannins from Selected Brown Macroalgae against the Cotton Leafhopper Amrasca devastans
Authors: Petchidurai Ganeshan , Sahayaraj Kitherian , Al-Shuraym Laila A. , Albogami Bader Z. , Sayed Samy M.
Published: September 2023

Seaweeds, also known as marine macroalgae, are renewable biological resources that are found worldwide and possess a wide variety of secondary metabolites, including tannins. Drifted brown seaweed (DBSW) is particularly rich in tannins and is regarded as biological trash. The cotton leaf hopper Amrasca devastans (Distant) has caused both quantitative and qualitative losses in cotton production. Drifted brown seaweeds (DBSWs) were used in this study to extract, qualitatively profile, and quantify the levels of total tannins, condensed tannins, hydrolyzable tannins, and phlorotannins in the seaweeds; test their insecticidal activity; and determine the mechanism of action. The largest amount of tannin extract was found in Sargassum wightii Greville (20.62%) using the Soxhlet method (SM). Significantly higher amounts of hydrolyzable tannins (p = 0.005), soluble phlorotannins (p = 0.005), total tannins in the SM (p = 0.003), and total tannins in the cold percolation method (p = 0.005) were recorded in S. wightii. However, high levels of condensed tannins (CTAs) were observed in Turbinaria ornata (Turner) J. Agardh (p = 0.004). A. devastans nymphs and adults were examined for oral toxicity (OT) and contact toxicity (CT) against DBSW tannin crude extract and column chromatographic fractions 1 (Rf = 0.86) and 2 (Rf = 0.88). Stoechospermum polypodioides (J.V. Lamouroux) J. Agardh crude tannin was highly effective against A. devastans using the OT method (LC50, 0.044%) when compared with the standard gallic acid (LC50, 0.044%) and tannic acid (LC50, 0.122%). Similarly, S. wightii fraction 2 (LC50, 0.007%) showed a greater insecticidal effect against A. devastans adults in OT than gallic acid (LC50, 0.034%) and tannic acid (LC50, 0.022%). The mechanism of action results show that A. devastans adults treated with crude tannin of T. ornata had significantly decreased amylase, protease (p = 0.005), and invertase (p = 0.003) levels when compared with the detoxification enzymes. The levels of glycosidase, lactate dehydrogenase, esterase, lipase, invertase, and acid phosphate activities (p = 0.005) of S. wightii were reduced when compared with those of the Vijayneem and chemical pesticide Monocrotophos. In adult insects treated with LC50 concentrations of S. wightii tannin fraction 1, the total body protein (9.00 µg/µL) was significantly reduced (OT, LC50—0.019%). The SDS-PAGE analysis results also show that S. wightii tannin fraction 1 (OT and CT), fraction 2 (OT), and S. polypodioides fraction 2 (CT) had a significant effect on the total body portion level, appearance, and disappearance of some proteins and polypeptides. This study shows that the selected brown macroalgae can be utilized for the safer management of cotton leaf hoppers.
Effect of NaCl Pretreatment on the Relationship between the Color Characteristics and Taste of Cirsium setidens Processed Using a Micro-Oil-Sprayed Thermal Air Technique
Authors: Kim Yonghyun , Lee Uk , Eo Hyun Ji
Published: September 2023

Cirsium setidens is commonly used as a food ingredient, and it is typically stored and distributed in a dried form to prolong its shelf life. In a previous study, a micro-oil-sprayed thermal air (MOTA) technique was developed, which effectively enhanced the rehydration properties and improved the color characteristics of Cirsium setidens after processing. Here, we investigated the relationship between the color characteristics and taste of MOTA-processed C. setidens and the effect of NaCl pretreatment, prior to processing, on the final quality of dried C. setidens. NaCl pretreatment, whether combined with the MOTA technique or not, showed improved color characteristics, in which MOTA-and NaCl+ MOTA-processed C. setidens manifested equal color characteristics. In contrast, NaCl + MOTA-processed C. setidens resulted in significantly higher values of sourness and saltiness than MOTA-processed C. setidens when the taste of the rehydrated C. setidens was examined using an electronic tongue (Astree II; Alpha MOS, Toulouse, France). Pearson correlation coefficient analysis showed that sourness and saltness were negatively correlated with Hunter a* values and positively correlated with Hunter L* and Hunter b* values, indicating that the color characteristics of dried and rehydrated C. setidens could be indicators of taste. Furthermore, the amounts of total phenol and chlorophyll were better preserved in C. setidens processed by the MOTA technique with NaCl than by the MOTA technique alone. Our data revealed that the color characteristics of dried plants are associated with the taste of processed C. setidens, and that the MOTA technique with NaCl pretreatment is a useful method for preserving health-promoting compounds during processing.
Comparative Evaluation of the In Vitro Cytotoxicity of a Series of Chitosans and Chitooligosaccharides Water-Soluble at Physiological pH
Authors: Dias Catia , Commin Loris , Bonnefont-Rebeix Catherine , Buff Samuel , Bruyère Pierre , Trombotto Stéphane
Published: September 2023

Chitosans (CS) have been of great interest due to their properties and numerous applications. However, CS have poor solubility in neutral and basic media, which limits their use in these conditions. In contrast, chitooligosaccharides (COS) have better solubility in water and lower viscosity in aqueous solutions whilst maintaining interesting biological properties. CS and COS, unlike other sugars, are not single polymers with a defined structure but are groups of molecules with modifiable structural parameters, allowing the adaptation and optimization of their properties. The great versatility of CS and COS makes these molecules very attractive for different applications, such as cryopreservation. Here, we investigated the effect of the degree of polymerization (DP), degree of N-acetylation (DA) and concentration of a series of synthesized CS and COS, water-soluble at physiological pH, on their cytotoxicity in an L929 fibroblast cell culture. Our results demonstrated that CS and COS showed no sign of toxicity regarding cell viability at low concentrations (≤10 mg/mL), independently of their DP and DA, whereas a compromising effect on cell viability was observed at a high concentration (100 mg/mL).
A SAS Macro for Automated Stopping of Markov Chain Monte Carlo Estimation in Bayesian Modeling with PROC MCMC
Authors: Wagner Wolfgang , Hecht Martin , Zitzmann Steffen
Published: September 2023

A crucial challenge in Bayesian modeling using Markov chain Monte Carlo (MCMC) estimation is to diagnose the convergence of the chains so that the draws can be expected to closely approximate the posterior distribution on which inference is based. A close approximation guarantees that the MCMC error exhibits only a negligible impact on model estimates and inferences. However, determining whether convergence has been achieved can often be challenging and cumbersome when relying solely on inspecting the trace plots of the chain(s) or manually checking the stopping criteria. In this article, we present a SAS macro called %automcmc that is based on PROC MCMC and that automatically continues to add draws until a user-specified stopping criterion (i.e., a certain potential scale reduction and/or a certain effective sample size) is reached for the chain(s).
Intercultural, Ecumenical and Interreligious Dialogue: An Introduction
Authors: Anthony Francis-Vincent
Published: September 2023

Religious traditions with their universal intent on human salvation or well-being have de-territorialized themselves or migrated from the place of their origin along trade routes in the company of merchants, invaders, and colonizers [...]
Special Issue Featuring Papers for Celebrating the Third Year since the Founding of Reproductive Medicine
Authors: Palomba Stefano
Published: September 2023

It is a great pleasure to introduce this Special Issue celebrating the third year since the founding of Reproductive Medicine [...]
CViTF-Net: A Convolutional and Visual Transformer Fusion Network for Small Ship Target Detection in Synthetic Aperture Radar Images
Authors: Huang Min , Liu Tianen , Chen Yazhou
Published: September 2023

Detecting small ship targets in large-scale synthetic aperture radar (SAR) images with complex backgrounds is challenging. This difficulty arises due to indistinct visual features and noise interference. To address these issues, we propose a novel two-stage detector, namely a convolutional and visual transformer fusion network (CViTF-Net), and enhance its detection performance through three innovative modules. Firstly, we designed a pyramid structured CViT backbone. This design leverages convolutional blocks to extract low-level and local features, while utilizing transformer blocks to capture inter-object dependencies over larger image regions. As a result, the CViT backbone adeptly integrates local and global information to bolster the feature representation capacity of targets. Subsequently, we proposed the Gaussian prior discrepancy (GPD) assigner. This assigner employs the discrepancy of Gaussian distributions in two dimensions to assess the degree of matching between priors and ground truth values, thus refining the discriminative criteria for positive and negative samples. Lastly, we designed the level synchronized attention mechanism (LSAM). This mechanism simultaneously considers information from multiple layers in region of interest (RoI) feature maps, and adaptively adjusts the weights of diverse regions within the final RoI. As a result, it enhances the capability to capture both target details and contextual information. We achieved the highest comprehensive evaluation results for the public LS-SSDD-v1.0 dataset, with an mAP of 79.7% and an F1 of 80.8%. In addition, the robustness of the CViTF-Net was validated using the public SSDD dataset. Visualization of the experimental results indicated that CViTF-Net can effectively enhance the detection performance for small ship targets in complex scenes.