bluuebunny/crossref_metadata_2025 huggingface dataset:
Preliminary Findings From a Pilot Study of Electric Vehicle Recharging From a Stand-Alone Solar Minigrid
Authors: Gammon Rupert , Sallah Momodou
Published: January 2021

The symbiosis between smart minigrids and electric mobility has the potential to improve the cost and reliability of energy access for off-grid communities while providing low-carbon transport services. This study explores the commercial viability of using electric vehicles (EVs), recharged by solar minigrids, to provide transport services in off-grid communities. Preliminary findings are presented from a field trial in The Gambia that aims to assess the techno-economic feasibility of integrating sustainable energy and transport infrastructures in sun-rich regions of the Global South. As a dispatchable anchor load, an EV can improve the technical and economic performance of a minigrid by providing demand-side response services. In the developing world, rural communities are often among the poorest, and inadequate transport services remain a major barrier to wealth-creation. Some solutions to this situation may be transferrable to island communities, which share similar challenges in terms of access to energy and fuel. The first of its kind in Africa, this field trial uses an electric minivan, operating from an off-grid village where it has access to a minigrid whose 4.5 kWpof photovoltaic modules form the roof of a parking shelter for the vehicle. While there, the taxi can recharge, ideally during sunny periods when the photovoltaic array produces surplus power, thus allowing the EV’s battery to recharge while bypassing the minigrid’s own accumulator. This improves system reliability and cost effectiveness, while providing pollution-free energy for the taxi. Ultimately, the intention is to test different vehicles in a variety of circumstances, but this paper outlines only the preliminary findings of the first of these trials. Early results provide convincing new evidence that commercial viability of such a concept is possible in Sub-Saharan Africa. Some promising scenarios for commercial viability are identified, which warrant further investigation, since they suggest that a taxi driver’s earnings could be increased between 250 and 1,300% in local operations, and even 20-fold in tourist markets, depending on vehicle type, minigrid configuration and target market. It is hoped that these may encourage the rollout of solar-recharged EVs where the nexus of sustainable energy and transport systems are likely to make the greatest contribution to addressing the UN’s Sustainable Development Goals, by helping to solve the trilemma of providing energy security, social benefit and environmental sustainability in low-income countries.
Effects of Demographic and Weather Parameters on COVID-19 Basic Reproduction Number
Authors: Salom Igor , Rodic Andjela , Milicevic Ognjen , Zigic Dusan , Djordjevic Magdalena , Djordjevic Marko
Published: January 2021

It is hard to overstate the importance of a timely prediction of the COVID-19 pandemic progression. Yet, this is not possible without a comprehensive understanding of environmental factors that may affect the infection transmissibility. Studies addressing parameters that may influence COVID-19 progression relied on either the total numbers of detected cases and similar proxies (which are highly sensitive to the testing capacity, levels of introduced social distancing measures, etc.), and/or a small number of analyzed factors, including analysis of regions that display a narrow range of these parameters. We here apply a novel approach, exploiting widespread growth regimes in COVID-19 detected case counts. By applying nonlinear dynamics methods to the exponential regime, we extract basic reproductive numberR0(i.e., the measure of COVID-19 inherent biological transmissibility), applying to the completely naïve population in the absence of social distancing, for 118 different countries. We then use bioinformatics methods to systematically collect data on a large number of potentially interesting demographics and weather parameters for these countries (where data was available), and seek their correlations with the rate of COVID-19 spread. While some of the already reported or assumed tendencies (e.g., negative correlation of transmissibility with temperature and humidity, significant correlation with UV, generally positive correlation with pollution levels) are also confirmed by our analysis, we report a number of both novel results and those that help settle existing disputes: the absence of dependence on wind speed and air pressure, negative correlation with precipitation; significant positive correlation with society development level (human development index) irrespective of testing policies, and percent of the urban population, but absence of correlation with population densityper se. We find a strong positive correlation of transmissibility on alcohol consumption, and the absence of correlation on refugee numbers, contrary to some widespread beliefs. Significant tendencies with health-related factors are reported, including a detailed analysis of the blood type group showing consistent tendencies on Rh factor, and a strong positive correlation of transmissibility with cholesterol levels. Detailed comparisons of obtained results with previous findings, and limitations of our approach, are also provided.
Recent Advances in Camel Immunology
Authors: Hussen Jamal , Schuberth Hans-Joachim
Published: January 2021

Camels are domesticated animals that are highly adapted to the extreme desert ecosystem with relatively higher resistance to a wide range of pathogens compared to many other species from the same geographical region. Recently, there has been increased interest in the field of camel immunology. As the progress in the analysis of camel immunoglobulins has previously been covered in many recent reviews, this review intends to summarize published findings related to camel cellular immunology with a focus on the phenotype and functionality of camel leukocyte subpopulations. The review also describes the impact of different physiological (age and pregnancy) and pathological (e.g. infection) conditions on camel immune cells. Despite the progress achieved in the field of camel immunology, there are gaps in our complete understanding of the camel immune system. Questions remain regarding innate recognition mechanisms, the functional characterization of antigen-presenting cells, and the characterization of camel NK and cytotoxic T cells.
Mitochondrial DNA Profiling to Combat the Illegal Trade in Tortoiseshell Products
Authors: LaCasella Erin L. , Jensen Michael P. , Madden Hof Christine A. , Bell Ian P. , Frey Amy , Dutton Peter H.
Published: January 2021

Hawksbill turtles (Eretmochelys imbricata) are exploited for their beautiful shell known as tortoiseshell or bekko, making them extremely vulnerable in the illegal global trade of tortoiseshell products. In this study, we developed an effective, standardized method using a commercially available kit to extract DNA and obtain informative mitochondrial DNA (mtDNA) control region sequences (~800 bp) from hawksbill turtle products in order to trace the sample back to a likely stock origin. We also sequenced additional skin samples from nesting beaches of Milman Island, Australia and Arnavon Island, Solomon Islands to add to the baseline data for hawksbill turtles in the Indo-Pacific. Our results indicate that nine of the 13 tortoiseshell products obtained from Papua New Guinea and Solomon Islands were from turtles with haplotypes found primarily at the Solomon Islands rookery and did not match those from nesting populations in Australia or SE Asia, with the exception of one haplotype also found in 3% of turtles at Milman Island. We also found that 23% of the market samples have haplotypes only documented in foraging populations, which illustrates the urgent need for more extensive sampling of rookeries to fill gaps in the reference baseline database. Nevertheless, our study results demonstrate an effective methodology for obtaining DNA of sufficient quantity and quality from hawksbill turtle products.
Extreme Levels of Ocean Acidification Restructure the Plankton Community and Biogeochemistry of a Temperate Coastal Ecosystem: A Mesocosm Study
Authors: Spisla Carsten , Taucher Jan , Bach Lennart T. , Haunost Mathias , Boxhammer Tim , King Andrew L. , Jenkins Bettany D. , Wallace Joselynn R. , Ludwig Andrea , Meyer Jana , Stange Paul , Minutolo Fabrizio , Lohbeck Kai T. , Nauendorf Alice , Kalter Verena , Lischka Silke , Sswat Michael , Dörner Isabel , Ismar-Rebitz Stefanie M. H. , Aberle Nicole , Yong Jaw C. , Bouquet Jean-Marie , Lechtenbörger Anna K. , Kohnert Peter , Krudewig Michael , Riebesell Ulf
Published: January 2021

The oceans’ uptake of anthropogenic carbon dioxide (CO2) decreases seawater pH and alters the inorganic carbon speciation – summarized in the term ocean acidification (OA). Already today, coastal regions experience episodic pH events during which surface layer pH drops below values projected for the surface ocean at the end of the century. Future OA is expected to further enhance the intensity of these coastal extreme pH events. To evaluate the influence of such episodic OA events in coastal regions, we deployed eight pelagic mesocosms for 53 days in Raunefjord, Norway, and enclosed 56–61 m3 of local seawater containing a natural plankton community under nutrient limited post-bloom conditions. Four mesocosms were enriched with CO2 to simulate extreme pCO2 levels of 1978 – 2069 μatm while the other four served as untreated controls. Here, we present results from multivariate analyses on OA-induced changes in the phyto-, micro-, and mesozooplankton community structure. Pronounced differences in the plankton community emerged early in the experiment, and were amplified by enhanced top-down control throughout the study period. The plankton groups responding most profoundly to high CO2 conditions were cyanobacteria (negative), chlorophyceae (negative), auto- and heterotrophic microzooplankton (negative), and a variety of mesozooplanktonic taxa, including copepoda (mixed), appendicularia (positive), hydrozoa (positive), fish larvae (positive), and gastropoda (negative). The restructuring of the community coincided with significant changes in the concentration and elemental stoichiometry of particulate organic matter. Results imply that extreme CO2 events can lead to a substantial reorganization of the planktonic food web, affecting multiple trophic levels from phytoplankton to primary and secondary consumers.
The Horizontal and Vertical Distribution of Deep-Sea Sediments Fungal Community in the South China Sea
Authors: Feng Li , Song Qianqian , Jiang Qun , Li Zhiyong
Published: January 2021

Although diverse fungi have been found in the deep-sea habitats, the space distribution of fungi has not been well characterized. In this study, the fungal horizontal and vertical distribution of the deep-sea sediments, four locations, three depths each, in the South China Sea, were compared using ITS2 high-throughput sequencing. It was revealed that the South China Sea deep-sea sediments harbor diverse marine fungi, including 82.39% Ascomycota, 8.10% Basidiomycota, 0.55% Zygomycota and 8.96% unknown fungi. The results indicate that fungal community structure is not uniform among the different sediment habitats. Though surface sediments have similar fungal diversity across the 4 locations, the fungal abundance and diversity increase with the depth of the sediments from 0 to 2 m, and 1 and 2 m deep sediments show obvious location-dependent fungal community structure. This is the first time to compare the horizontal and vertical distribution of fungal community among different deep-sea sediments in the South China Sea by high-throughput sequencing, providing novel insights into the space distribution characteristics of deep-sea sediments fungi.
Motor Progression in Early-Stage Parkinson's Disease: A Clinical Prediction Model and the Role of Cerebrospinal Fluid Biomarkers
Authors: Ma Ling-Yan , Tian Yu , Pan Chang-Rong , Chen Zhong-Lue , Ling Yun , Ren Kang , Li Jing-Song , Feng Tao
Published: January 2021

Background: The substantial heterogeneity of clinical symptoms and lack of reliable progression markers in Parkinson's disease (PD) present a major challenge in predicting accurate progression and prognoses. Increasing evidence indicates that each component of the neurovascular unit (NVU) and blood-brain barrier (BBB) disruption may take part in many neurodegenerative diseases. Since some portions of CSF are eliminated along the neurovascular unit and across the BBB, disturbing the pathways may result in changes of these substances.

Methods: Four hundred seventy-four participants from the Parkinson's Progression Markers Initiative (PPMI) study (NCT01141023) were included in the study. Thirty-six initial features, including general information, brief clinical characteristics and the current year's classical scale scores, were used to build five regression models to predict PD motor progression represented by the coming year's Unified Parkinson's Disease Rating Scale (MDS-UPDRS) Part III score after redundancy removal and recursive feature elimination (RFE)-based feature selection. Then, a threshold range was added to the predicted value for more convenient model application. Finally, we evaluated the CSF and blood biomarkers' influence on the disease progression model.

Results: Eight hundred forty-nine cases were included in the study. The adjusted R2 values of three different categories of regression model, linear, Bayesian and ensemble, all reached 0.75. Models of the same category shared similar feature combinations. The common features selected among the categories were the MDS-UPDRS Part III score, Montreal Cognitive Assessment (MOCA) and Rapid Eye Movement Sleep Behavior Disorder Questionnaire (RBDSQ) score. It can be seen more intuitively that the model can achieve certain prediction effect through threshold range. Biomarkers had no significant impact on the progression model within the data in the study.

Conclusions: By using machine learning and routinely gathered assessments from the current year, we developed multiple dynamic models to predict the following year's motor progression in the early stage of PD. These methods will allow clinicians to tailor medical management to the individual and identify at-risk patients for future clinical trials examining disease-modifying therapies.
Effectiveness of Cyanoacrylate in Reducing Seroma Formation in Breast Cancer Patients Post-Axillary Dissection: A Randomized Controlled Trial
Authors: Al-Masri Mahmoud , Alawneh Fade , Daoud Faiez , Ebous Ali , Hamdan Basem , Al-Najjar Hani , Al-Masri Rama , Abufara Marwan
Published: January 2021

Background/Purpose

Seroma is a common complication after axillary dissection in women with node-positive breast cancer. We aim to determine the effect of Cyanoacrylate on reducing seroma formation in patients undergoing axillary dissection. This a randomized clinical trial.

Methods

This is a single-center, randomized, single-blinded, and two-arm parallel study. Women with node-positive breast cancer eligible for axillary dissection were enrolled. Patients with a Body Mass Index (BMI) greater than 35 kg/m2, those who underwent immediate breast reconstruction, and/or received neoadjuvant chemotherapy were excluded. Patients were randomized in a 1:1 ratio, and were stratified according to their age, BMI, tumor size, and operation type. The primary endpoint was the total seroma volume (the total drained volume and the total aspirated volume after drain removal). Data presented as mean and range when applicable.

Results

111 patients were randomized (Cyanoacrylate 57; control 54). 105 patients were analyzed. Sixty-nine patients underwent breast conserving surgery, and 36 underwent modified radical mastectomy. There was no difference in the total seroma volume between the Cyanoacrylate vs. control arms (1,304 (60–4,950) vs. 1,446 (100–5,223) ml, p=0.458). Wound infection, flap necrosis, number of manual aspirates, and hematoma formation were not statistically different between the two groups. Time to drain removal was shorter in the Cyanoacrylate arm (11.04(3–23) vs. 13.84(3–37) days, p=0.015). The use of Cyanoacrylate was not cost effective ($586.93 (550–748) vs. $29.63 (0–198), p<0.001). Higher seroma volume was correlated with modified radical mastectomy, older age, and BMI more than 30 kg/m2.

Conclusion

Cyanoacrylate did not reduce seroma formation and its use was not cost effective.

Clinical Trial Registration

clinicaltrials.gov, identifier NCT02141373.
Elevated Heterogeneous Nuclear Ribonucleoprotein C Expression Correlates With Poor Prognosis in Patients With Surgically Resected Lung Adenocarcinoma
Authors: Guo Wei , Huai Qilin , Zhang Guochao , Guo Lei , Song Peng , Xue Xuemin , Tan Fengwei , Xue Qi , Gao Shugeng , He Jie
Published: January 2021

Background

Lung adenocarcinoma (LUAD), as the most common histological subtype of lung cancer, is a high-grade malignancy and a leading cause of cancer-related death globally. Identification of biomarkers with prognostic value is of great significance for the diagnosis and treatment of LUAD. Heterogeneous nuclear ribonucleoprotein C (HNRNPC) is an RNA-binding protein “reader” of N6-methyladenosine (m6A) methylation, and is related to the progression of various cancers; however, its role in LUAD is unclear. The aims of this study aims were to study the expression and prognostic value of HNRNPC in LUAD.

Methods

The Oncomine database and gene expression profiling interactive analysis (GEPIA) were used for preliminary exploration of HNRNPC expression and prognostic value in LUAD. LUAD cases from The Cancer Genome Atlas (TCGA) (n = 416) and the Kaplan-Meier plotter database (n = 720) were extracted to study the differential expression and prognostic value of HNRNPC. HNRNPC expression in the National Cancer Center of China (NCC) cohort was analyzed by immunohistochemical staining, and the relationship between HNRNPC expression and survival rate evaluated using the Kaplan-Meier method and log-rank test. Univariate and multivariate Cox regression analyses were used to identify independent prognostic factors. Several pathways that were significantly enriched in the HNRNPC high expression group were identified by Gene Set Enrichment Analysis (GSEA).

Results

Five data sets from the Oncomine and GEPIA databases all supported that HNRNPC expression is significantly higher in LUAD than in normal lung tissue. In TCGA cohort, HNRNPC was highly expressed in LUAD tissues and significantly related to age, sex, smoking history, ethnicity, lymph node metastasis, and TNM staging (P < 0.001). High HNRNPC expression was significantly correlated with poor prognosis in the three cohorts (NCC, TCGA, and K-M plotter) (P < 0.05). Multivariate Cox regression analysis showed that HNRNPC expression was an independent prognostic factor in both TCGA and NCC cohorts (P < 0.05). Further, 10 significantly enriched pathways were identified from TCGA data and 118 lung cancer cell lines in CCLE, respectively.

Conclusions

High HNRNPC expression is significantly related to poor overall survival in patients with LUAD, suggesting that HNRNPC may be a cancer-promoting factor and a potential prognostic biomarker in LUAD.
Application of Gastroscopy in the Diagnosis of Congenital Pyriform Sinus Fistula in Children
Authors: Wang Shengcai , Mei Lin , Li Yanzhen , Zhang Xuexi , Zhang Jie , Ge Wentong , Guo Yongli , Yu Yongbo , Wang Guoli , Mei Tianlu , Liu Qiaoyin , Sun Nian , He Yuzhu , Li Xiaodan , Liu Yuwei , Tai Jun , Ni Xin
Published: January 2021

Objective: The aim of this study was to explore the diagnostic value of gastroscopy under local anesthesia for congenital pyriform sinus fistula (CPSF).

Methods: This research was a diagnostic study. Patients received gastroscopy under local anesthesia to diagnose CPSF, and suspension laryngoscopy under general anesthesia was performed 2 days after gastroscopy. Various conditions of the internal opening of CPSF were then recorded. Patients were grouped according to lesion sides, age, time after the inflammation subsided, and history of previous surgery. The sensitivity, specificity, area under the receiver operating characteristic curve (AUC), accuracy, and positive and negative predictive values of gastroscopy were compared between the groups.

Results: A total of 48 patients were recruited in this study, and no patients had severe gastroscopy-related complications. The diagnostic values of gastroscopy in 41 cases (85.4%) were consistent with suspension laryngoscopy. The sensitivity of gastroscopy was 86.4%, the specificity was 75%, the AUC was 0.807, the positive prediction rate was 97.4%, the negative prediction rate was 33.3%, the accuracy rate was 85.4%, and the diagnostic odds ratio (DOR) was 2.1. The kappa consistency test results had statistical significance (P = 0.0026, kappa = 0.3913). The diagnostic value of gastroscopy was better for the patients with inflammation subsiding for more than 4 weeks (P < 0.0001).

Conclusion: Gastroscopy under local anesthesia is a safe, effective, reliable and novel diagnostic method for CPSF, and it is especially recommended as a diagnostic method for the patients with inflammation subsiding for more than 4 weeks.
Clinical Classifications of Children With Psychogenic Non-epileptic Seizure
Authors: Zhang Li-Ping , Jia Yu , Huang Hao , Li Da-Wei , Wang Yu-Ping
Published: January 2021

Objective: To analyze the clinical features of children with psychogenic non-epileptic seizures in one tertiary center in China.

Methods: Clinical data including medical records and video- electroencephalograph (video-EEG) monitoring records of 88 pediatric PNES patients hospitalized in the pediatric department of Xuanwu Hospital, Beijing, China from April, 2012 to April, 2018 were collected in this study. Demographic information of patients, semiological classification, duration, and frequency of symptoms, risk factors as well as comorbidity were summarized and analyzed.

Results: For semiological classification, all PNES related symptoms were divided into different categories: motor symptoms, unresponsiveness, sensory symptoms, visceral symptoms, and abnormal behaviors, among which motor symptoms were the most prevalent form. Risk factors were reviewed and categorized into two groups: persistent factors and predisposing factors, and patients were most frequently affected by the influences of families. The duration and frequency of symptoms varied substantially within PNES patients while the average time of duration was relatively longer than epilepsy as reported previously. Epilepsy was considered as the most frequent comorbidity of PNES and PNES patients misdiagnosed as epilepsy often mistreated with antiseizure medication.

Significance: Our study showed that motor PNES are the most frequent seizure type. Family issues were a risk factor for PNES. Epilepsy was the most frequent co-existing neurological comorbidity.
Aloperine Relieves Type 2 Diabetes Mellitus via Enhancing GLUT4 Expression and Translocation
Authors: Song Guanjun , Huang Yun , Xiong Mingrui , Yang Ziwei , Liu Qinghua , Shen Jinhua , Zhao Ping , Yang Xinzhou
Published: January 2021

Aloperine (ALO), a quinolizidine alkaloid isolated from Sophora alopecuroides L. used in the traditional Uygur medicine, induced a significant increase in cellular glucose uptake of L6 cells, suggesting it has the potential to relieve hyperglycemia. Therefore, we investigated the effects of ALO on type 2 diabetes mellitus (T2DM) through in vitro and in vivo studies. The translocation of glucose transporter 4 (GLUT4) and changes in intracellular Ca2+ levels were real-time monitored in L6 cells using a laser scanning confocal microscope and related protein kinase inhibitors were used to explore the mechanism of action of ALO. Furthermore, high fat diet combined with low-dose streptozotocin (STZ) was used to induce T2DM in rats, and ALO was given to the stomach of T2DM rats for 4 weeks. In vitro results showed that ALO-induced enhancement of GLUT4 expression and translocation were mediated by G protein-PLC-PKC and PI3K/Akt pathways and ALO-enhanced intracellular Ca2+ was involved in activating PKC via G protein-PLC-IP3R-Ca2+ pathway, resulting in promoted GLUT4 plasma membrane fusion and subsequent glucose uptake. ALO treatment effectively ameliorated hyperglycemia, glucose intolerance, insulin resistance and dyslipidemia, alleviated hepatic steatosis, protected pancreatic islet function and activated GLUT4 expression in insulin target tissues of T2DM rats. These findings demonstrated that ALO deserves attention as a potential hypoglycemic agent.
Synthesis, Characterization, Cellular Uptake, and In Vitro Anticancer Activity of Fullerenol-Doxorubicin Conjugates
Authors: Xu Beihua , Yuan Li , Hu Ying , Xu Zhiyuan , Qin Jiang-Jiang , Cheng Xiang-Dong
Published: January 2021

Doxorubicin (DOX) is one of the most commonly used chemotherapeutic agents for treating human cancer. However, its clinical use has been limited by DOX-induced cardiotoxicity as well as other side effects. In the present study, we designed and synthesized the fullerenol (FU)-DOX conjugates and folic acid (FA)-grafted FU-DOX conjugates for improving the selectivity and activity of DOX in cancer cells. We further characterized the physicochemical properties and examined the release kinetics, cellular uptake, and in vitro anticancer activities of FU-DOX and FA-FU-DOX. The results showed that FU-DOX and FA-FU-DOX had a mean diameter of <200 nm and a low polydispersity. Both FU-DOX and FA-FU-DOX exhibited pH sensitivity and their DOX release rates were higher at pH 5.9 vs. pH 7.4. The cellular uptake studies indicated that FU conjugation enhanced the intracellular accumulation of DOX in human hepatocellular carcinoma (HCC) cell lines (BEL-7402 and HepG2) and the immortalized normal human hepatocytes (L02). The conjugation of FA to FU-DOX further promoted the drug internalization in an FR-dependent manner and enhanced the cytotoxicity against HCC cells. In conclusion, the newly prepared FA-FU-DOX conjugates can optimize the safety and efficacy profile of DOX.
Geriatric Nutritional Risk Index as a Prognostic Marker for Patients With Metastatic Castration-Resistant Prostate Cancer Receiving Docetaxel
Authors: Chang Li-Wen , Hung Sheng-Chun , Li Jian-Ri , Chiu Kun-Yuan , Yang Cheng-Kuang , Chen Chuan-Shu , Lu Kevin , Chen Cheng-Che , Wang Shu-Chi , Lin Chia-Yen , Cheng Chen-Li , Ou Yen-Chuan , Yang Shun-Fa , Hsu Chiann-Yi , Ho Szu-Hang , Wang Shian-Shiang
Published: January 2021

Purpose: To investigate the prognostic efficacy of the Geriatric Nutritional Risk Index (GNRI) in patients with metastatic Castration–resistant Prostate Cancer (mCRPC) receiving docetaxel as the first line of treatment.

Methods: We retrospectively reviewed patients with mCRPC and receiving first line docetaxel in Taichung Veterans General Hospital from 2006 to 2012. The GNRI was calculated using serum albumin and body mass index, with a poor nutritional status defined as GNRI <92.0. Multivariate Cox-regression analysis was used to evaluate the risk of survival.

Results: One-hundred seventy patients with mCRPC were included. One-hundred twenty-five patients were of normal nutritional status (GNRI ≥92) and 45 patients were of poor nutritional status (GNRI <92). The cumulative docetaxel dosage was 600 (360–1,185) mg in the normal nutritional status group and 360 (127.5–660) mg in the poor nutritional status group (p < 0.001). The median overall survival from mCRPC was 30.39 months in the good nutritional status group and 11.07 months in the poor nutritional status group (p of log rank <0.001). In a multivariate model, poor nutritional status was an independent risk factor in overall survival (Hazard Ratio [HR] = 5.37, 95% Confidence Interval [CI] 3.27–8.83), together with a high metastatic volume (HR = 4.03, 95% CI 2.16–7.53) and docetaxel cumulative dosage (HR = 0.999, 95% CI 0.999–0.9998).

Conclusion: Poor nutritional status with a GNRI <92 is associated with shorter progression free survival and overall survival in mCRPC patients treated with docetaxel. Metastatic volume and cumulative docetaxel dosage are also independent prognostic factors in overall survival.
Floral Induction in the Short-Day Plant Chrysanthemum Under Blue and Red Extended Long-Days
Authors: SharathKumar Malleshaiah , Heuvelink Ep , Marcelis Leo F. M. , van Ieperen Wim
Published: January 2021

Shorter photoperiod and lower daily light integral (DLI) limit the winter greenhouse production. Extending the photoperiod by supplemental light increases biomass production but inhibits flowering in short-day plants such as Chrysanthemum morifolium. Previously, we reported that flowering in growth-chamber grown chrysanthemum with red (R) and blue (B) LED-light could also be induced in long photoperiods by applying only blue light during the last 4h of 15h long-days. This study investigates the possibility to induce flowering by extending short-days in greenhouses with 4h of blue light. Furthermore, flower induction after 4h of red light extension was tested after short-days RB-LED light in a growth-chamber and after natural solar light in a greenhouse. Plants were grown at 11h of sole source RB light (60:40) in a growth-chamber or solar light in the greenhouse (short-days). Additionally, plants were grown under long-days, which either consisted of short-days as described above extended with 4h of B or R light to long-days or of 15h continuous RB light or natural solar light. Flower initiation and normal capitulum development occurred in the blue-extended long-days in the growth-chamber after 11h of sole source RB, similarly as in short-days. However, when the blue extension was applied after 11h of full-spectrum solar light in a greenhouse, no flower initiation occurred. With red-extended long-days after 11h RB (growth-chamber) flower initiation occurred, but capitulum development was hindered. No flower initiation occurred in red-extended long-days in the greenhouse. These results indicate that multiple components of the daylight spectrum influence different phases in photoperiodic flowering in chrysanthemum in a time-dependent manner. This research shows that smart use of LED-light can open avenues for a more efficient year-round cultivation of chrysanthemum by circumventing the short-day requirement for flowering when applied in emerging vertical farm or plant factories that operate without natural solar light. In current year-round greenhouses’ production, however, extension of the natural solar light during the first 11 h of the photoperiod with either red or blue sole LED light, did inhibit flowering.
From Affective Arrangements to Affective Milieus
Authors: Schuetze Paul
Published: January 2021

In this paper, I develop the concept ofaffective milieusby building on the recently established notion ofaffective arrangements. Affective arrangements bring together the more analytical research of situated affectivity with affect studies informed by cultural theory. As such, this concept takes a step past the usual synchronic understanding of situatedness toward an understanding of the social, dynamic, historical, and cultural situatedness of individuals in relation to situated affectivity. However, I argue that affective arrangements remain too narrow in their scope of analysis since their focus mainly lies on local, marked-off, and unique constellations of affect relations. They neglect the more mundane and day-to-day affect dynamics of social life. Hence, I introduce the notion of affective milieus, which brings to light the everyday, ubiquitous affective engagements of individuals with their socio-material surroundings. Affective milieus specifically call attention to how commonplace affect relations create territories in the social universe which form and mold individuals all the time. In that way, this paper apprehends and advances recent developments in the research on situated affectivity.
The Impact of the Economic Corridor on Economic Stability: A Double Mediating Role of Environmental Sustainability and Sustainable Development Under the Exceptional Circumstances of COVID-19
Authors: Li Haiyan , Hameed Javaria , Khuhro Rafique Ahmed , Albasher Gadah , Alqahtani Wedad , Sadiq Muhammad Waqas , Wu Tong
Published: January 2021

This study discusses the impact of different economic indicators on economic stability, including honest leadership, improved infrastructure, revenue generation, and CPEC taking into account the double mediating role of environmental sustainability and sustainable development, while considering the latest COVID-19 situation. This study adopted primary data collection methods and obtained data from the employees of CPEC by using questionnaires and smart-PLS for analysis purposes. The results revealed that honest leadership, improved infrastructure, revenue generation, and CPEC have a positive nexus with economic stability. Despite the severe impact of COVID-19 on the country’s economy, the economic corridor plays a vital role in stabilizing the state’s economy and supports all those related to this phenomenal project either directly or indirectly.
Using Crowd-Sourced Speech Data to Study Socially Constrained Variation in Nonmodal Phonation
Authors: Gittelson Ben , Leemann Adrian , Tomaschek Fabian
Published: January 2021

This study examines the status of nonmodal phonation (e.g. breathy and creaky voice) in British English using smartphone recordings from over 2,500 speakers. With this novel data collection method, it uncovers effects that have not been reported in past work, such as a relationship between speakers’ education and their production of nonmodal phonation. The results also confirm that previous findings on nonmodal phonation, including the greater use of creaky voice by male speakers than female speakers, extend to a much larger and more diverse sample than has been considered previously. This confirmation supports the validity of using crowd-sourced data for phonetic analyses. The acoustic correlates that were examined include fundamental frequency, H1*-H2*, cepstral peak prominence, and harmonic-to-noise ratio.
Driving and Dissipation of Solar-Wind Turbulence: What is the Evidence?
Authors: Smith Charles W. , Vasquez Bernard J.
Published: January 2021

Fifty years of solar wind observations have provided extensive data that drives an evolving view of the fundamental nature and dynamics of the magnetic, velocity, and density fluctuations that are ubiquitous throughout the heliosphere. Despite the ongoing examination of ever improving data, fundamental questions remain unanswered because there are very few multi-point measurements from a sufficient number of spacecraft in close proximity to fully resolve the three-dimensional dynamics that are at the heart of the problem. Simulations provide new insights and new questions, but most simulations sacrifice one aspect of plasma physics in order to address another. Computers and computational methods remain insufficient to simulate fully compressive, fully nonlinear, collisionless plasma dynamics with sufficient spatial range and dimension to be considered a complete description of solar wind turbulence. For these reasons, there remain multiple divergent opinions as to the underlying dynamics of solar wind turbulence, dissipation, and the observed heating of the thermal plasma. We review observations of solar wind turbulence in so far as they contribute to an understanding of solar wind heating through the existence of energy reservoirs, the dynamics that move energy from the reservoirs to the dissipation scales, and the conversion into heat of energy associated with coherent fluctuations.
Development and Application of a Water Temperature Related Physiologically Based Pharmacokinetic Model for Enrofloxacin and Its Metabolite Ciprofloxacin in Rainbow Trout
Authors: Yang Fan , Yang Fang , Wang Dan , Zhang Chao-Shuo , Wang Han , Song Zhe-Wen , Shao Hao-Tian , Zhang Mei , Yu Meng-Li , Zheng Yang
Published: January 2021

Enrofloxacin (ENR) has been approved for the treatment of infections in aquaculture, but it may cause tissue residue. This research aimed to develop and validate a water temperature related PBPK model, including both ENR and ciprofloxacin (CIP), in rainbow trout, and to predict further their residue concentrations and the withdrawal periods for ENR at different water temperatures. With the published concentrations data, a flow-limited PBPK model including both ENR and CIP sub-models was developed to predict ENR and CIP concentrations in tissues and plasma/serum after intravenous, oral, or immersion administration. A Monte Carlo simulation including 500 iterations was further incorporated into this model. Based on the model and Monte Carlo analysis, the withdrawal intervals were estimated for different dosage regimens and at different water temperatures, ranging from 80 to 272 degree-days. All of these values were shorter than the labeled withdrawal period (500 degree-days) in fish. This model provided a useful tool for predicting the tissue residues of ENR and CIP in rainbow trout under different dosage regimens and at different water temperatures.
Learning from Incidents: A Qualitative Study in the Continuing Airworthiness Sector
Authors: Clare James , Kourousis Kyriakos
Published: January 2021

Learning from incidents (LFI) is a useful approach when examining past events and developing measures to prevent ensuing recurrence. Although the reporting of incidents in the aircraft maintenance and continuing airworthiness domain is well appointed, it is often unclear how the maximum effect of safety data can be efficaciously applied in support of LFI in the area. From semi-structured interviews, with thirty-four participants, the gathered data were thematically analyzed with the support of NVivo software. This study establishes a relationship between an incident in its lifecycle and the learning process. The main aim of this work is to elucidate factors that enable LFI. The analysis of the data revealed, for example, the benefits of a just culture and the use of formal continuation training programs in this respect. Moreover, it identified limitations inherent in current processes such as poor event causation and poorly designed learning syllabi. Additionally, aspects such as a lack of regulatory requirements for competence in the areas of learning for managers and accountable persons currently exist. This thematic analysis could be used in support of organizations examining their own processes for learning from incidents. Additionally, it can support the development of terms of reference for a continuing airworthiness regulatory working group to examine, strengthen and better apply LFI in the aviation industry.
Effects of Carbon Ion Beam Irradiation on Phenotypic Variations and Biochemical Parameters in Early Generations of Soybean Plants
Authors: Wang Xue , Liu Chang-Kai , Tu Bing-Jie , Li Yan-Sheng , Zhang Qiu-Ying , Liu Xiao-Bing
Published: January 2021

Understanding the characteristics of carbon ion beam irradiation-induced mutation is essential to its potential application in plant breeding. A carbon ion beam-mutagenized soybean population was generated from the newly released soybean variety Dongsheng 28, with irradiation dosages of 100 Gy, 120 Gy and 140 Gy. Many phenotypic variations and novel mutants with heritable tendencies including plant height mutants, sterile mutants, early mature mutants, rolled leaves and short petioles mutants, yield-related mutants and lodging-resistant mutants were identified. Diverse variations in seed size, seed protein and oil concentration were found. Increasing irradiation dosage from 100 Gy to 140 Gy increased leaf chlorophyll concentration in M1 generation, but this effect was significantly reduced in M2 generation. The activities of superoxide dismutase (SOD), peroxidase (POD) and malondialdehyde (MDA) concentration all showed wider variation in M1 and M2 generation, the only exception being that the MDA concentration was similar to the control in the M2 generation. Overall, we suggest that treating soybean seeds with carbon ion beam irradiation at a dosage of 120 Gy (80 Mev/u) could be effective in soybean mutation breeding.
Assessing the Impact of COVID-19 on Antimicrobial Stewardship Activities/Programs in the United Kingdom
Authors: Ashiru-Oredope Diane , Kerr Frances , Hughes Stephen , Urch Jonathan , Lanzman Marisa , Yau Ting , Cockburn Alison , Patel Rakhee , Sheikh Adel , Gormley Cairine , Chavda Aneeka , Vaghela Tejal , Phillips Ceri , Reid Nicholas , Brady Aaron
Published: January 2021

Since first identified in late 2019, the acute respiratory syndrome coronavirus (SARS-CoV2) and the resulting coronavirus disease (COVID-19) pandemic has overwhelmed healthcare systems worldwide, often diverting key resources in a bid to meet unprecedented challenges. To measure its impact on national antimicrobial stewardship (AMS) activities, a questionnaire was designed and disseminated to antimicrobialstewardship leads in the United Kingdom (UK). Most respondents reported a reduction in AMS activity with 64% (61/95) reporting that COVID-19 had a negative impact on routine AMS activities. Activities reported to have been negatively affected by the pandemic include audit, quality improvement initiatives, education, AMS meetings, and multidisciplinary working including ward rounds. However, positive outcomes were also identified, with technology being increasingly used as a tool to facilitate stewardship, e.g., virtual meetings and ward rounds and increased the acceptance of using procalcitonin tests to distinguish between viral and bacterial infections. The COVID-19 pandemic has had a significant impact on the AMS activities undertaken across the UK. The long-term impact of the reduced AMS activities on incidence of AMR are not yet known. The legacy of innovation, use of technology, and increased collaboration from the pandemic could strengthen AMS in the post-pandemic era and presents opportunities for further development of AMS.
Acknowledgment to Reviewers of Antibiotics in 2020
Authors:   Antibiotics Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Antibiotics maintains its standards for the high quality of its published papers [...]
Analysis of Lipid Peroxidation by UPLC-MS/MS and Retinoprotective Effects of the Natural Polyphenol Pterostilbene
Authors: Torres-Cuevas Isabel , Millán Iván , Asensi Miguel , Vento Máximo , Oger Camille , Galano Jean-Marie , Durand Thierry , Ortega Ángel L.
Published: January 2021

The loss of redox homeostasis induced by hyperglycemia is an early sign and key factor in the development of diabetic retinopathy. Due to the high level of long-chain polyunsaturated fatty acids, diabetic retina is highly susceptible to lipid peroxidation, source of pathophysiological alterations in diabetic retinopathy. Previous studies have shown that pterostilbene, a natural antioxidant polyphenol, is an effective therapy against diabetic retinopathy development, although its protective effects on lipid peroxidation are not well known. Plasma, urine and retinas from diabetic rabbits, control and diabetic rabbits treated daily with pterostilbene were analyzed. Lipid peroxidation was evaluated through the determination of derivatives from arachidonic, adrenic and docosahexaenoic acids by ultra-performance liquid chromatography coupled with tandem mass spectrometry. Diabetes increased lipid peroxidation in retina, plasma and urine samples and pterostilbene treatment restored control values, showing its ability to prevent early and main alterations in the development of diabetic retinopathy. Through our study, we are able to propose the use of a derivative of adrenic acid, 17(RS)-10-epi-SC-Δ15-11-dihomo-IsoF, for the first time, as a suitable biomarker of diabetic retinopathy in plasmas or urine.
Alpha-Tocopherol Metabolites (The Vitamin E Metabolome) and Their Interindividual Variability during Supplementation
Authors: Bartolini Desirée , Marinelli Rita , Giusepponi Danilo , Galarini Roberta , Barola Carolina , Stabile Anna Maria , Sebastiani Bartolomeo , Paoletti Fabiola , Betti Michele , Rende Mario , Galli Francesco
Published: January 2021

The metabolism of α-tocopherol (α-TOH, vitamin E) shows marked interindividual variability, which may influence the response to nutritional and therapeutic interventions with this vitamin. Recently, new metabolomics protocols have fostered the possibility to explore such variability for the different metabolites of α-TOH so far identified in human blood, i.e., the “vitamin E metabolome”, some of which have been reported to promote important biological functions. Such advances prompt the definition of reference values and degree of interindividual variability for these metabolites at different levels of α-TOH intake. To this end, a one-week oral administration protocol with 800 U RRR-α-TOH/day was performed in 17 healthy volunteers, and α-TOH metabolites were measured in plasma before and at the end of the intervention utilizing a recently validated LC-MS/MS procedure; the expression of two target genes of α-TOH with possible a role in the metabolism and function of this vitamin, namely pregnane X receptor (PXR) and the isoform 4F2 of cytochrome P450 (CYP4F2) was assessed by immunoblot in peripheral blood leukocytes. The levels of enzymatic metabolites showed marked interindividual variability that characteristically increased upon supplementation. With the exception of α-CEHC (carboxy-ethyl-hydroxychroman) and the long-chain metabolites M1 and α-13′OH, such variability was found to interfere with the possibility to utilize them as sensitive indicators of α-TOH intake. On the contrary, the free radical-derived metabolite α-tocopheryl quinone significantly correlated with the post-supplementation levels of α-TOH. The supplementation stimulated PXR, but not CYP4F2, expression of leucocytes, and significant correlations were observed between the baseline levels of α-TOH and both the baseline and post-supplementation levels of PXR. These findings provide original analytical and molecular information regarding the human metabolism of α-TOH and its intrinsic variability, which is worth considering in future nutrigenomics and interventions studies.
Investigating the Effect of Individual Rotations on the Physical and Physiological Performance in Elite Female Field Hockey Players
Authors: McGuinness Aideen , Kenna Darren , Grainger Adam , Collins Kieran
Published: January 2021

To quantify the rotational demands of elite female field hockey with respect to position. Twenty-eight (n = 28) elite international field hockey players were recruited during the 2018–2020 seasons. Players were monitored with GPS technology and heart-rate monitors. Methods: Activity was categorised into total distance (m), relative total distance (m·min−1), high-speed distance (m; 16 km∙h−1), relative high-speed distance (m·min−1), max velocity (km·h−1), and percentage maximal velocity (%Vmax). Physiological demands were assessed via heart rate measures (bpm and % HRmax) and time > 80% heart rate maximum. Results: A single rotation equated to 7 ± 0.8 min. Players covered a mean total distance of was 868 ± 132 m (125.7 ± 5.9 m·min−1) with 140 ± 39 m at high-speed (21.7 ± 3.6 m·min−1). A significant difference was reported for relative total (p ≤ 0.001), and high-speed (p ≤ 0.001), distance across positional. Forwards were reported to cover the most relative total and high-speed distance (d = 1.0) when compared to defenders and midfielders. Conclusion: The study provides normative data for rotational demands of elite female field hockey. Coaches should consider these demands when developing training drills to better optimise the positional physical and physiological demands of competitive match-play.