bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthesis of Ordered Mesoporous Molecular Sieve-Supported Cobalt Catalyst via Organometallic Complexation for Propane Non-Oxidative Dehydrogenation
Authors: Zhai Yanliang , Chen Lisha , Wu Ruihan , Lu Xianggang , Wang Jun , Li Gaolong , Tang Bicheng , Zhang Wei , Zhang Shaolong , Li Zhijun
Published: July 2024

Co-based catalysts have shown great promise for propane dehydrogenation (PDH) reactions due to their merits of environmental friendliness and low cost. In this study, ordered mesoporous molecular sieve-supported CoOx species (CoOx/Al-SBA-15 catalyst) were prepared by one-step organometallic complexation. The catalysts show worm-like morphology with regular straight-through mesoporous pores and high external specific surface area. These typical features can substantially enhance the dispersion of CoOx species and mass transfer of reactants and products. Compared with the conventional impregnation method, the 10CSOC (10 wt.% Co/Al-SBA-15 prepared by the organometallic complexation method) sample presents a smaller CoOx size and higher Co2+/Co3+ ratio. When applied to PDH reaction, the 10CSOC delivers higher propane conversion and propylene selectivity. Under the optimal conditions (625 °C and 4500 h−1), 10CSOC achieves high propane conversion (43%) and propylene selectivity (83%). This is attributed to the smaller and better dispersion of CoOx nanoparticles, more suitable acid properties, and higher content of Co2+ species. This work paves the way for the rational design of high-performance catalysts for industrially important reactions.
Rocket Dynamics of Capped Nanotubes: A Molecular Dynamics Study
Authors: Hamad Mustafa S. , Morciano Matteo , Fasano Matteo
Published: July 2024

The study of nanoparticle motion has fundamental relevance in a wide range of nanotechnology-based fields. Molecular dynamics simulations offer a powerful tool to elucidate the dynamics of complex systems and derive theoretical models that facilitate the invention and optimization of novel devices. This research contributes to this ongoing effort by investigating the motion of one-end capped carbon nanotubes within an aqueous environment through extensive molecular dynamics simulations. By exposing the carbon nanotubes to localized heating, propelled motion with velocities reaching up to ≈0.08 nm ps−1 was observed. Through systematic exploration of various parameters such as temperature, nanotube diameter, and size, we were able to elucidate the underlying mechanisms driving propulsion. Our findings demonstrate that the propulsive motion predominantly arises from a rocket-like mechanism facilitated by the progressive evaporation of water molecules entrapped within the carbon nanotube. Therefore, this study focuses on the complex interplay between nanoscale geometry, environmental conditions, and propulsion mechanisms in capped nanotubes, providing relevant insights into the design and optimization of nanoscale propulsion systems with various applications in nanotechnology and beyond.
Relationship between Village Chicken Availability and Dietary Diversity along a Rural–Urban Gradient
Authors: Mseleku Cresswell , Chimonyo Michael , Slotow Rob , Ngidi Mjabuliseni S.
Published: July 2024

Dietary diversity is one of the fundamental factors of nutritional security and a proxy used to measure diet quality. The objective of this study was to investigate the relationship between village chicken availability and the dietary diversity of households along a rural–urban gradient. Face-to-face interviews were conducted using a structured questionnaire in rural (n = 100), peri-urban (n = 100), and urban (n = 100) areas of Pietermaritzburg uMgungundlovu District, KwaZulu-Natal, in South Africa. A positive relationship between distance from the city center and village chicken flock sizes (p < 0.001) was observed. Consumption of vegetables increased with an increase in distance from the city center (p < 0.01). A quadratic relationship was observed between distance from the city center and consumption of livestock-derived foods (LDFs) (p < 0.05). Consumption of LDFs increased with an increase in village chicken flock sizes (p < 0.05). Consumption of vegetables increased with an increase in village chicken flock sizes (p < 0.01). Food variety score (FVS) increased with an increase in distance from the city center (p < 0.05). Assessing the availability of village chickens across rural–urban gradients is a worthy opportunity to utilize to improve households’ dietary diversity and alleviate poverty. It can be concluded that expanding village flock sizes could enhance the dietary diversity of households.
A Multi-Omics Approach to Disclose Metabolic Pathways Impacting Intestinal Permeability in Obese Patients Undergoing Very Low Calorie Ketogenic Diet
Authors: Celano Giuseppe , Calabrese Francesco Maria , Riezzo Giuseppe , D’Attoma Benedetta , Ignazzi Antonia , Di Chito Martina , Sila Annamaria , De Nucci Sara , Rinaldi Roberta , Linsalata Michele , Apa Carmen Aurora , Mancini Leonardo , De Angelis Maria , Giannelli Gianluigi , De Pergola Giovanni , Russo Francesco
Published: July 2024

A very low calorie ketogenic diet (VLCKD) impacts host metabolism in people marked by an excess of visceral adiposity, and it affects the microbiota composition in terms of taxa presence and relative abundances. As a matter of fact, there is little available literature dealing with microbiota differences in obese patients marked by altered intestinal permeability. With the aim of inspecting consortium members and their related metabolic pathways, we inspected the microbial community profile, together with the set of volatile organic compounds (VOCs) from untargeted fecal and urine metabolomics, in a cohort made of obese patients, stratified based on both normal and altered intestinal permeability, before and after VLCKD administration. Based on the taxa relative abundances, we predicted microbiota-derived metabolic pathways whose variations were explained in light of our cohort symptom picture. A totally different number of statistically significant pathways marked samples with altered permeability, reflecting an important shift in microbiota taxa. A combined analysis of taxa, metabolic pathways, and metabolomic compounds delineates a set of markers that is useful in describing obesity dysfunctions and comorbidities.
Coeliac Disease and Microscopic Colitis: The Largest Study Assessing Prognosis and Risk of Hospital Admission
Authors: Raju Suneil , Rawcliffe Megan , Bowker-Howell Freya , Shiha Mohamed , Kaur Kamaldeep , Griffin Jonathan , Cross Simon , Sanders David
Published: July 2024

Microscopic colitis (MC) and coeliac disease (CD) are common associated gastrointestinal conditions. We present the largest study assessing hospitalisation in patients with MC and the effect of a concomitant diagnosis of CD. Data were retrospectively collected between January 2007 and December 2021 from all patients diagnosed with MC and compared to a database of patients with only CD. In total, 892 patients with MC (65% female, median age 65 years (IQR: 54–74 years) were identified, with 6.4% admitted to hospital due to a flare of MC. Patients admitted were older (76 vs. 65 years, p < 0.001) and presented with diarrhoea (87.7%), abdominal pain (26.3%), and acute kidney injury (17.5%). Treatment was given in 75.9% of patients, including intravenous fluids (39.5%), steroids (20.9%), and loperamide (16.3%). Concomitant CD was diagnosed in 3.3% of patients and diagnosed before MC (57 versus 64 years, p < 0.001). Patients with both conditions were diagnosed with CD later than patients with only CD (57 years versus 44 years, p < 0.001). In conclusion, older patients are at a higher risk of hospitalisation due to MC, and this is seen in patients with a concomitant diagnosis of CD too. Patients with MC are diagnosed with CD later than those without.
Preoperative Sarcopenia Severity and Clinical Outcomes after Total Hip Arthroplasty
Authors: Tanaka Shinya , Kayamoto Azusa , Terai Chiaki , Nojiri Shusuke , Fugane Yuki , Mori Tomohiro , Nagaya Motoki , Kako Masato , Iida Hiroki , Osawa Yusuke , Takegami Yasuhiko , Nishida Yoshihiro
Published: July 2024

The outcome of total hip arthroplasty (THA) in patients with end-stage arthritis of the hip is associated with preoperative physical status. This study was performed to examine the relationship between the preoperative severity of sarcopenia and clinical outcomes after THA. This retrospective cohort study was performed among 306 consecutive patients (mean age: 63.7 ± 12.9 years, 222 women) undergoing THA at a university hospital. The severity of sarcopenia was determined based on the skeletal muscle mass index (SMI), handgrip strength, and gait speed according to the criteria of the Asian Working Group for Sarcopenia 2019. The severe sarcopenia prevalence rate was 10.6%. Severe sarcopenia was significantly associated with the risk of delayed functional recovery (adjusted odds ratio, 2.82; 95% confidence interval, 1.03–7.72; p = 0.043) compared with the non-sarcopenia group after adjusting for pre-existing risk factors, including preoperative hip function and physical activity. The addition of SMI, handgrip strength, and gait speed to the model for risk of functional recovery delay significantly increased the area under the receiver operating characteristic curve (p = 0.038). Severe sarcopenia was significantly associated with poorer hip function and patient-reported outcomes at 6 months after surgery compared with the non-sarcopenia group. Severe sarcopenia was adversely associated with postoperative clinical outcomes in patients undergoing THA.
Effect of Crocus sativus Extract Supplementation in the Metabolic Control of People with Diabetes Mellitus Type 1: A Double-Blind Randomized Placebo-Controlled Trial
Authors: Giannoulaki Parthena , Kotzakioulafi Evangelia , Nakas Alexandros , Kontoninas Zisis , Karlafti Eleni , Evripidou Polykarpos , Kantartzis Konstantinos , Savopoulos Christos , Chourdakis Michail , Didangelos Triantafyllos
Published: July 2024

Introduction–Background: Data from experimental trials show that Crocus sativus L. (saffron) is considered to improve glycemia, lipid profile, and blood pressure and reduce oxidative stress. So far, clinical trials have been conducted in individuals with metabolic syndrome and Diabetes Mellitus type 2 (DMT-2). The purpose of this study is to assess the effectiveness of saffron in individuals with Diabetes Mellitus type 1 (DMT-1). Patients–Methods: 61 individuals with DMT-1, mean age 48 years old (48.3 ± 14.6), 26 females (42.6%) were randomized to receive a new oral supplement in sachets containing probiotics, prebiotics, magnesium, and Crocus sativus L. extract or placebo containing probiotics, prebiotics and magnesium daily for 6 months. Glycemic control was assessed with a continuous glucose monitoring system and laboratory measurement of HbA1c and lipid profile was also examined. Blood pressure at baseline and end of intervention was also measured. Individuals were either on a continuous subcutaneous insulin infusion with an insulin pump or in multiple daily injection regimens. Diabetes distress and satiety were assessed through a questionnaire and body composition was assessed with bioelectrical impedance. Results: At the end of the intervention, the two groups differed significantly only in serum triglycerides (p = 0.049). After 6 months of treatment, a significant reduction in the active group was observed in glycated hemoglobin (p = 0.046) and serum triglycerides (p = 0.021) compared to baseline. The other primary endpoints (glycemic control, lipid profile, blood pressure) did not differ within the groups from baseline to end of intervention, and there was no significant difference between the two groups. Diabetes distress score improved significantly only in the active group (p = 0.044), suggesting an overall improvement in diabetes disease burden in these individuals but that was not significant enough between the two groups. Conclusions: A probiotic supplement with saffron extract improves serum triglycerides in well-controlled people with DMT-1 and may potentially be a valuable adjunct for enhancing glycemic control.
Importance of Gut Microbiota in Patients with Inflammatory Bowel Disease
Authors: Ekstedt Natalia , Jamioł-Milc Dominika , Pieczyńska Joanna
Published: July 2024

Inflammatory bowel diseases (IBDs), such as Crohn’s disease (CD) and ulcerative colitis (UC), are chronic diseases of the digestive system with a multifactorial and not fully understood etiology. There is research suggesting that they may be initiated by genetic, immunological, and lifestyle factors. In turn, all of these factors play an important role in the modulation of intestinal microflora, and a significant proportion of IBD patients struggle with intestinal dysbiosis, which leads to the conclusion that intestinal microflora disorders may significantly increase the risk of developing IBD. Additionally, in IBD patients, Toll-like receptors (TLRs) produced by intestinal epithelial cells and dendritic cells treat intestinal bacterial antigens as pathogens, which causes a disruption of the immune response, resulting in the development of an inflammatory process. This may result in the occurrence of intestinal dysbiosis, which IBD patients are significantly vulnerable to. In this study, we reviewed scientific studies (in particular, systematic reviews with meta-analyses, being studies with the highest level of evidence) regarding the microflora of patients with IBD vs. the microflora in healthy people, and the use of various strains in IBD therapy.
Probiotic Supplementation in the Neonatal Age Group and the Risk of Hospitalisation in the First Two Years: A Data Linkage Study from Western Australia
Authors: Srinivasjois Ravisha , Gebremedhin Amanuel , Silva Desiree , Rao Shripada C. , Tessema Gizachew A. , Pereira Gavin
Published: July 2024

Background: Probiotic supplementation in preterm neonates is standard practice in many centres across the globe. The impact of probiotic supplementation in the neonatal age group on the risk of hospitalisation in infancy has not been reported previously. Methods: Infants born < 32 + 6 weeks of gestation in Western Australia were eligible for inclusion. We conducted a retrospective cohort study comparing data from before probiotic supplementation (Epoch 1: 1 December 2008–30 November 2010, n = 1238) versus after (Epoch 2: 1 June 2012–30 May 2014, n = 1422) on the risks of respiratory- and gastrointestinal infection–related hospitalisation. A subgroup analysis of infants born < 28 weeks of gestation was analysed separately for similar outcomes. Results: Compared to Epoch 1, an 8% reduction in incidence of hospitalisation up to 2 years after birth was observed in Epoch 2 (adjusted incidence rate ratio (IRR) of 0.92; 95% confidence interval (CI); 0.87–0.98), adjusted for gestational age, smoking, socioeconomic status, and maternal age. The rate of hospitalisation for infants born < 28 weeks of gestation was comparable in epochs 1 and 2. Conclusion: Infants exposed to probiotic supplementation in the neonatal period experience a reduced risk of hospitalisation in the first two years after discharge from the neonatal unit.
Peripheral Biomarkers of Anorexia Nervosa: A Meta-Analysis
Authors: Wu Ya-Ke , Watson Hunna J. , Del Re Aaron C. , Finch Jody E. , Hardin Sabrina L. , Dumain Alexis S. , Brownley Kimberly A. , Baker Jessica H.
Published: July 2024

The pathogenesis of anorexia nervosa (AN) has been hypothesized to involve several biological systems. However, reliable biomarkers for AN have yet to be established. This study was aimed to identify statistically significant and clinically meaningful peripheral biomarkers associated with AN. A systematic literature search was conducted to identify studies published in English from inception until 30 June 2022. We conducted two-level random-effects meta-analyses to examine the difference between AN and comparison groups across 52 distinct biomarkers and found that acylated ghrelin, adrenocorticotropic hormone (ACTH), carboxy-terminal collagen crosslinks (CTX), cholesterol, cortisol, des-acyl ghrelin, ghrelin, growth hormone (GH), obestatin, and soluble leptin receptor levels were significantly higher in cases of AN compared with those in non-AN controls. Conversely, C-reactive protein (CRP), CD3 positive, CD8, creatinine, estradiol, follicle-stimulating hormone (FSH), free thyroxine, free triiodothyronine, glucose, insulin, insulin-like growth factor 1 (IGF-1), leptin, luteinizing hormone, lymphocyte, and prolactin levels were significantly lower in AN compared with those in non-AN controls. Our findings indicate that peripheral biomarkers may be linked to the pathophysiology of AN, such as processes of adaptation to starvation. Scientific investigation into peripheral biomarkers may ultimately yield breakthroughs in personalized clinical care for AN.
Should Empiric Anti-Fungals Be Administered Routinely for All Patients with Perforated Peptic Ulcers? A Critical Review of the Existing Literature
Authors: Chan Kai Siang , Tan Lee Yee Calista , Balasubramaniam Sunder , Shelat Vishal G.
Published: July 2024

A perforated peptic ulcer (PPU) is a surgical emergency with a high mortality rate. PPUs cause secondary peritonitis due to bacterial and fungal peritoneal contamination. Surgery is the main treatment modality and patient’s comorbidites impacts perioperative morbidity and surgical outcomes. Even after surgery, resuscitation efforts should continue. While empiric antibiotics are recommended, the role of empiric anti-fungal treatment is unclear due to a lack of scientific evidence. This literature review demonstrated a paucity of studies evaluating the role of empiric anti-fungals in PPUs, and with conflicting results. Studies were heterogeneous in terms of patient demographics and underlying surgical pathology (PPUs vs. any gastrointestinal perforation), type of anti-fungal agent, timing of administration and duration of use. Other considerations include the need to differentiate between fungal colonization vs. invasive fungal infection. Despite positive fungal isolates from fluid culture, it is important for clinical judgement to identify the right group of patients for anti-fungal administration. Biochemistry investigations including new fungal biomarkers may help to guide management. Multidisciplinary discussions may help in decision making for this conundrum. Moving forward, further research may be conducted to select the right group of patients who may benefit from empiric anti-fungal use.
Simulation and Modeling of the Adhesion of Staphylococcus aureus onto Inert Surfaces under Fluid Shear Stress
Authors: Shaikh Sarees , Saleem Abdul Nafay , Ymele-Leki Patrick
Published: July 2024

Bacterial adhesion to biotic and abiotic surfaces under fluid shear stress plays a major role in the pathogenesis of infections linked to medical implants and tissues. This study employed an automated BioFlux 200 microfluidic system and video microscopy to conduct real-time adhesion assays, examining the influence of shear stress on adhesion kinetics and spatial distribution of Staphylococcus aureus on glass surfaces. The adhesion rate exhibited a non-linear relationship with shear stress, with notable variations at intermediate levels. Empirical adhesion events were simulated with COMSOL Multiphysics® and Python. Overall, COMSOL accurately predicted the experimental trend of higher rates of bacterial adhesion with decreasing shear stress but poorly characterized the plateauing phenomena observed over time. Python provided a robust mathematical representation of the non-linear relationship between cell concentration, shear stress, and time but its polynomial regression approach was not grounded on theoretical physical concepts. These insights, combined with advancements in AI and machine learning, underscore the potential for synergistic computational techniques to enhance our understanding of bacterial adhesion to surfaces, offering a promising avenue for developing novel therapeutic strategies.
MaMYBR30, a Novel 1R-MYB, Plays Important Roles in Plant Development and Abiotic Stress Resistance
Authors: Liu Li , Li Shan , Tang Fengjuan , Li Peijun , Liu Jiaxin , Fu Rumeng , Zheng Longyan , Zhang Jie , Chao Nan
Published: July 2024

The V-myb myeloblastosis viral oncogene homolog (MYB) family participate in various bioprocesses including development and abiotic stress responses. In the present study, we first report a 1R SHAQKYF-class MYB, MaMYBR30, in mulberry. Subcellular localization and sequence analysis indicated MaMYBR30 is located in the nucleus and belongs to a CCA-like subgroup with a conserved SHAQKYF motif. Expression profile analysis showed that MaMYBR30 is expressed in leaves and can be induced by drought and salt stress. The down-regulation of MaMYBR30 using virus-induced gene silence (VIGS) in mulberry and the overexpression of MaMYBR30 in Arabidopsis were induced to explore the function of MaMYBR30. The functional characterization of MaMYBR30 in vivo indicated that MaMYBR30 can positively regulate the resistance of mulberry to drought while negatively regulating the resistance of mulberry to salt stress. In addition, MaMYBR30 also affects flower development and reproductive growth, especially after exposure to salt stress. Weighted gene co-expression network analysis (WGCNA) primarily revealed the possible genes and signal pathways that are regulated by MaMYBR30. Our results also imply that complex molecular mechanisms mediated by MaMYBR30, including crosstalk of ion toxicity, phytohormone signal transduction, flowering development, and epigenetic modification, need to be further explored in the future.
First Clarification of the Mechanism of Action of the Apple Glycosyltransferase MdUGT91AJ2 Involved in the Detoxification Metabolism of the Triketone Herbicide Sulcotrione
Authors: Zhao Aijuan , Teng Xiao , Ma Yingxin , Mu Lijun , Han Shibo , Wang Shumin , Lei Kang , Ji Lusha , Li Pan
Published: July 2024

Sulcotrione is a member of triketone herbicides, a class of HPPD (4-hydroxyphenylpyruvate dioxygenase) inhibitors with broad-spectrum herbicidal activity. Modifications of glycosylation mediated by glycosyltransferases (GT) are involved in plant detoxification. In this study, we analyzed chip data published online and found that eight glycosyltransferases from group A of the apple glycosyltransferase family 1 may be involved in the metabolic mechanism of detoxification of triketone herbicides. To verify this prediction, we induced apple seedlings with six types of triketone herbicides, and then detected the expression levels of eight glycosyltransferase genes through real-time PCR. We found that triketone herbicides induced up-regulation of eight glycosyltransferase genes to varying degrees, with MdUGT91AJ2 being the most significantly up-regulated by sulcotrione-induced glycosyltransferase gene expression. Then, through in vitro enzymatic reactions and HPLC identification of glycoside substrates, it was found that the glycosyltransferase MdUGT91AJ2 had the highest specific enzyme activity against the triketone herbicide sulcotrione. Furthermore, the in vivo mechanism of the glycosyltransferase MdUGT91AJ2 in the detoxification metabolism of sulcotrione was further validated by overexpressing the strain in the plant. HPLC analysis showed that the content of sulcotrione glycosides in the overexpressing strain of MdUGT91AJ2 was significantly higher than that in the wild type. This result indicated that the apple glycosyltransferase MdUGT91AJ2 can still glycosylate and modify sulfotrione in plants, and participate in its detoxification metabolism. In summary, this study identified for the first time a novel apple glycosyltransferase MdUGT91AJ2 and elucidated its mechanism of action in the detoxification and metabolism of the triketone herbicide sulfotriene.
Application and Experimental Substantiation of the Radioecological Model for Prediction in Behavior 90Sr in Cultivated Soil-Crop System: A Case Study of Two Experimental Agricultural Fields
Authors: Sarap Nataša B. , Daković Marko Ž. , Djalovic Ivica , Dolijanović Željko , Prasad P.V. Vara , Janković Marija M.
Published: July 2024

The radioactive fission product 90Sr has a sufficient half-life (28.8 years) to be detected long after its appearance in the environment. After its uptake into the soil-edible plant system, it enters the food chain and represents a potential source of contamination that threatens human health. Due to these facts, tracking the distribution of the artificial radionuclide 90Sr in the soil–edible plant system is a subject of intense research. The tracking of the 90Sr radionuclide distribution in the soil profile, as well as in the crops on the long-term experimental fields was carried out using beta radiation spectrometry. The radiochemical analytical method was used to analyze the 90Sr content in cultivated soil and crops. The conducted study focused on the experimental substantiation of the developed model for predicting the behavior of 90Sr in the cultivated soil–crop system. The results of using the applied radioecological model for the transfer of 90Sr from the soil to the above-ground part of crops showed a relatively good agreement with the experimentally determined values of the soil–crop transfer factor, which indicates that the used model can be successfully applied for the prediction of the behavior of 90Sr in the soil–soil solution–crop system.
Chemical Composition and In Vitro Bioactivities of Extracts from Cones of P. halepensis, P. brutia, and P. pinea: Insights into Pharmaceutical and Cosmetic Potential
Authors: Chammam Amel , Fillaudeau Luc , Romdhane Mehrez , Bouajila Jalloul
Published: July 2024

Various parts of the Pinaceae species, a traditional plant, have potential health benefits and exhibit antibacterial, anti-cancer, and antioxidant activities. This study aims to investigate the biochemical properties of both petal (P) and core (C) fractions from pinecones of P. halepensis (PA), P. brutia (PB), and P. pinea (PP). Pinecones were manually separated into P and C, which were then milled to investigate maceration with solvents of increasing polarity: cyclohexane (1SV), ethyl acetate (2SV), and methanol (3SV) at 20 °C. Spectrophotometry was utilized to quantify the total phenolic content (TPC) and to assess bioactivities. Gas chromatography with mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) were employed to identify the chemical composition. 3SV extracts demonstrated the highest TPC and a significant anti–oxidant potential. PA-P-3SV exhibited the highest TPC (460.66 mg GAE/g DW) and PP-P-3SV displayed the best IC50 (10.54 µg/mL) against DPPH. 1SV and 2SV extracts showed interesting anticancer activity against Hela and HepG2 cells. No significant toxic effect of P and C extracts from pinecones was observed on HEK-293 cells. GC-MS analysis unveiled 46 volatile compounds, of which 32 were detected for the first time in these species. HPLC analysis identified 38 compounds, of which 27 were not previously detected in these species. This study highlights the significant potential of pinecones as a rich source of bioactive compounds.
Quantitative Trait Loci Sequencing and Genetic Mapping Reveal Two Main Regulatory Genes for Stem Color in Wax Gourds
Authors: Chen Zhihao , Wang Peng , Bai Wenhui , Deng Yan , Cheng Zhikui , Su Liwen , Nong Lifeng , Liu Ting , Yang Wenrui , Yang Xiping , Liu Zhengguo
Published: July 2024

Stem color is an important agronomic trait of wax gourds. However, its regulatory genes have not been identified. In this study, 105 inbred lines constructed from two parents (GX-71 and MY-1) were sequenced and quantitative trait loci sequencing was used to mine the genes that regulate stem color in wax gourds. The results identified two quantitative trait loci related to stem color, qSC5 and qSC12, located on Chr05 (11,134,567–16,459,268) and Chr12 (74,618,168–75,712,335), respectively. The qSC5 had a phenotypic variation rate of 36.9% and a maximum limit of detection of 16.9. And the qSC12 had a phenotypic variation rate of 20.9%, and a maximum limit of detection of 11.2. Bch05G003950 (named BchAPRR2) and Bch12G020400 were identified as candidate genes involved in stem color regulation in wax gourds. The chlorophyll content and expression of BchAPRR2 and Bch12G020400 were significantly higher in green-stemmed wax gourds than in white-stemmed ones. Therefore, BchAPRR2 and Bch12G020400 were considered the main and secondary regulatory genes for wax gourd stem color, respectively. Finally, InDel markers closely linked to BchAPRR2 were developed to validate the prediction of wax gourd stem color traits in 55 germplasm lines, with an accuracy of 81.8%. These findings lay the foundation for exploring the genetic regulation of wax gourd stem color and future research on wax gourd breeding.
Straw Retention with Reduced Fertilization Enhances Soil Properties, Crop Yields, and Emergy Sustainability of Wheat–Soybean Rotation
Authors: Yu Qi , Jiao Xiaoying , Wang Chenyu , Wang Yanbo , Xu Xiyang , Liu Zhenyuan , Ren Guangxin , Feng Yongzhong
Published: July 2024

Cereal + legume rotation is an integrated system that facilitates soil fertility and sustainable agricultural production. However, research on the management compatibility affecting soil physico-chemical properties yields overall agro-ecosystem sustainability, but profitability is lacking, especially under straw retention and potential reductions in fertilizer application. An 11-year field experiment investigated three treatments: no straw retention + traditional mineral fertilization (TNS), straw retention + traditional mineral fertilization (TS), and straw retention + reduced mineral fertilization (DS). Compared with TNS, TS significantly improved soil physico-chemical properties, including macro-aggregates (R > 0.25 mm), porosity, field water capacity (FWC), soil organic carbon (SOC) storage, total nitrogen storage, microbial biomass carbon (MBC), and microbial biomass nitrogen (MBN) by 17.3%, 3.2%, 13.0%, 5.5%, 3.2%, 15.5%, and 13.8%, respectively. TS also significantly increased total (wheat + soybean) yields (TYs), economic profits, and emergy sustainability index (ESI) by 15.8%, 25.0%, 3.7 times that of TNS, respectively. Surprisingly, compared with TS, DS further significantly improved R > 0.25 mm, porosity, FWC, SOC storage, MBC, MBN, TY, economic profits, and ESI by 11.4%, 1.5%, 6.1%, 3.0%, 10.6%, 7.2%, 5.7%, 11.1%, and 36.5%, respectively. Overall, retaining straw with reduced fertilization enhances soil properties, yields, and emergy sustainability in wheat–soybean rotation systems.
Diversity and Pathogenicity of Botryosphaeriaceae Species Isolated from Olives in Istria, Croatia, and Evaluation of Varietal Resistance
Authors: Petrović Elena , Vrandečić Karolina , Belušić Vozila Andreina , Ćosić Jasenka , Godena Sara
Published: July 2024

During 2021 and 2022, a field investigation was conducted in Istria, Croatia, searching for trees exhibiting signs of Botryosphaeria dieback. Samples of symptomatic trees were collected from 26 different locations and analysed. Isolates that morphologically corresponded to species from the Botryosphaeriaceae family were selected, and detailed morphological characterisation and molecular identification of the isolates were conducted. Based on morphological characteristics and phylogenetic analysis using the internal transcribed spacer (ITS), beta-tubulin (TUB2), and translation elongation factor 1-alpha (TEF1-α) regions, six species of fungi from the Botryosphaeriaceae family were identified: Botryosphaeria dothidea (Moug. ex Fr.) Ces. & De Not.; Diplodia mutila (Fr.) Fr.; Diplodia seriata De Not.; Dothiorella iberica A.J.L. Phillips, J. Luque & A. Alves; Dothiorella sarmentorum (Fr.) A.J.L. Phillips, Alves & Luque; and Neofusicoccum parvum (Pennycook & Samuels) Crous, Slippers & A.J.L. Phillips. This is the first report of D. mutila, Do. sarmentorum, and Do. iberica causing Botryosphaeria dieback on olive trees in Croatia, and the first study investigating the resistance of Croatian olive varieties to species from the Botryosphaeriaceae family. Pathogenicity testing of selected isolates and assessment of variety resistance were conducted on four different olive varieties, namely Buža, Istarska bjelica, Leccino, and Rosinjola, using representative isolates of the mentioned species. The most aggressive species was found to be N. parvum. Olive varieties exhibited differences in susceptibility depending on the fungus they were infected with.
Molecular Dynamic Simulations for Biopolymers with Biomedical Applications
Authors: Garduño-Juárez Ramón , Tovar-Anaya David O. , Perez-Aguilar Jose Manuel , Lozano-Aguirre Beltran Luis Fernando , Zubillaga Rafael A. , Alvarez-Perez Marco Antonio , Villarreal-Ramirez Eduardo
Published: July 2024

Computational modeling (CM) is a versatile scientific methodology used to examine the properties and behavior of complex systems, such as polymeric materials for biomedical bioengineering. CM has emerged as a primary tool for predicting, setting up, and interpreting experimental results. Integrating in silico and in vitro experiments accelerates scientific advancements, yielding quicker results at a reduced cost. While CM is a mature discipline, its use in biomedical engineering for biopolymer materials has only recently gained prominence. In biopolymer biomedical engineering, CM focuses on three key research areas: (A) Computer-aided design (CAD/CAM) utilizes specialized software to design and model biopolymers for various biomedical applications. This technology allows researchers to create precise three-dimensional models of biopolymers, taking into account their chemical, structural, and functional properties. These models can be used to enhance the structure of biopolymers and improve their effectiveness in specific medical applications. (B) Finite element analysis, a computational technique used to analyze and solve problems in engineering and physics. This approach divides the physical domain into small finite elements with simple geometric shapes. This computational technique enables the study and understanding of the mechanical and structural behavior of biopolymers in biomedical environments. (C) Molecular dynamics (MD) simulations involve using advanced computational techniques to study the behavior of biopolymers at the molecular and atomic levels. These simulations are fundamental for better understanding biological processes at the molecular level. Studying the wide-ranging uses of MD simulations in biopolymers involves examining the structural, functional, and evolutionary aspects of biomolecular systems over time. MD simulations solve Newton’s equations of motion for all-atom systems, producing spatial trajectories for each atom. This provides valuable insights into properties such as water absorption on biopolymer surfaces and interactions with solid surfaces, which are crucial for assessing biomaterials. This review provides a comprehensive overview of the various applications of MD simulations in biopolymers. Additionally, it highlights the flexibility, robustness, and synergistic relationship between in silico and experimental techniques.
Calibration of Thermal Viscoelastic Material Models for the Dynamic Responses of PVB and SG Interlayer Materials
Authors: Knight Jon , Salim Hani , Elemam Hesham , Elbelbisi Ahmed
Published: July 2024

Laminated glass interlayer materials polyvinyl butyral (PVB) and SentryGlas® (SG, kuraray, Houstan, TX, USA) exhibit thermal viscoelastic behavior under dynamic tensile loading. Significant temperature and strain rate effects on the behavior of these interlayer materials pose a challenge for accurately modeling the dynamic response of laminated glass. Many researchers have simplified their approaches by modeling the response of the interlayer material using a bilinear approximation or established hyperelastic models. However, temperature and strain rate effects can be captured using the three-network viscoplastic (TNV) model. Therefore, the objective of this study is to calibrate material models for the thermal viscoelastic dynamic responses of PVB and SG interlayer materials. Uniaxial tensile tests were performed at strain rates of 2, 20, and 45 s−1 and temperatures of 0, 23, and 60 °C, and material models were calibrated using the experimental data. Finite element analysis using the calibrated material models successfully predicted the dynamic responses of PVB and SG under the experimental test conditions within a 10% error margin. This suggests that the calibrated models using the TNV model represent significant improvements over existing approaches to modeling the dynamic response of laminated glass. Similar procedures can be applied to other thermoplastics, laying the groundwork for establishing a standard calibration guide.
Effective Unidirectional Wetting of Liquids on Multi-Gradient, Bio-Inspired Surfaces Fabricated by 3D Printing and Surface Modification
Authors: Hsu Che-Ni , Mai Ngoc Phuong Uyen , Chang Haw-Kai , Chen Po-Yu
Published: July 2024

The movement of liquid droplets on the energy gradient surface has attracted extensive attention inspired by biological features in nature, such as the periodic spindle-shaped nodes in spider silks and conical-like barbs of cacti, and the structure–property–function relationship of multifunctional gradient surfaces. In this study, a series of specific patterns are fabricated with 3D printing technology, followed by modification via the atmospheric pressure plasma treatment and liquid phase chemical deposition, resulting in enhancing the ability of water droplets of 5 μL to travel 18.47 mm on a horizontal plane and 22.75 mm against gravity at up to a 20° tilting angle. Additionally, analysis techniques have been employed, including a contact angle analyzer, ESCA, and a laser confocal microscope to evaluate the sample performance. This work could further be applied to many applications related to microfluidic devices, drug delivery and water/fog collection.
Degradation of Waste Tetra Pak Packaging with Hydrothermal Treatment in Sub-/Supercritical Water
Authors: Irgolič Mihael , Čolnik Maja , Kotnik Petra , Škerget Mojca
Published: July 2024

Tetra pak packaging is one of the most frequently used types of packaging in the food industry. The recycling of the tetra pak packaging waste presents a difficult task because of its multi-layered, multi-component structure. In this study, the degradation of tetra pak packaging in subcritical (SubCW) and supercritical (SCW) water was investigated. The experiments were carried out in one (SCW) or two stages (SubCW and SCW), whereby the influence of the reaction temperature and time on the yield and composition of the products obtained was investigated. The maximum oil phase yield achieved in a one-stage and a two-stage degradation process was 60.7% and 65.5%, respectively. The oil and gas phases were composed of different types of hydrocarbons. Higher temperature and longer time led to higher amounts of saturated aliphatic hydrocarbons in both the oil and gas phases. The aqueous phase contained sugars (glucose, fructose) and sugar derivatives (levulinic acid, glyceraldehyde, furfurals). Based on these results, the degradation pathway of waste tetra pak packaging in SubCW and SCW was proposed. The results of the study show that the degradation of waste tetra pak packaging with SubCW and SCW is a promising recycling process.
Exploring the Impact of Bio-Based Plasticizers on the Curing Behavior and Material Properties of a Simplified Tire-Tread Compound
Authors: van Elburg Frances , Grunert Fabian , Aurisicchio Claudia , di Consiglio Micol , di Ronza Raffaele , Talma Auke , Bernal-Ortega Pilar , Blume Anke
Published: July 2024

The tire industry needs to become more sustainable to reduce pollution and fight climate change. Replacing fossil ingredients in a tire-tread compound with bio-based alternatives is an approach to create a more sustainable product. For instance, the plasticizer can be replaced, which is a petroleum-based ingredient used in relatively high amounts in the rubber. This approach was followed in the current study. Three plant-based plasticizers were selected as potential substitutes for treated distillate aromatic extract (TDAE) in a simplified tire-tread compound formulation, namely, sunflower oil, coconut oil, and cardanol. Additionally, squalane was used as a TDAE replacement to further investigate the possible interactions between plasticizers and other compound ingredients. Squalane (C30H62) is a fully saturated substance, containing six methyl groups but no additional chemical functional groups. Therefore, it was expected that squalane would result in limited interactions within the studied system. All alternatives to TDAE showed an increased cure rate and decreased scorch time, except squalane. This indicates that the three bio-based plasticizers might interact with the vulcanization system. For example, they could function as an additional coactivator of the curing system and/or shield the silica surface. A severe decrease in maximum torque and an increase in elongation at break were obtained for cardanol and sunflower oil. Both plasticizers also resulted in lower crosslink densities compared to the other compounds. A model study with the bio-plasticizers and sulfur verified that the unsaturation in the cardanol and sunflower oil reacted with the crosslinking agent. This leads to less sulfur available for the curing reaction, explaining the low maximum torque. The tan δ curves showed that all replacements resulted in a decrease in the glass transition temperature of the compound. Although all oil alternatives displayed promising results, none of them are suitable as a direct substitute for TDAE in a tire-tread compound due to its ability to interact additionally with other rubber ingredients and contribute in this form to the reinforcement of the compound.
EUNet: Edge-UNet for Accurate Building Extraction and Edge Emphasis in Gaofen-7 Images
Authors: Han Ruijie , Fan Xiangtao , Liu Jian
Published: July 2024

Deep learning is currently the mainstream approach for building extraction tasks in remote-sensing imagery, capable of automatically learning features of buildings in imagery and yielding satisfactory extraction results. However, due to the diverse sizes, irregular layouts, and complex spatial relationships of buildings, extracted buildings often suffer from incompleteness and boundary issues. Gaofen-7 (GF-7), as a high-resolution stereo mapping satellite, provides well-rectified images from its rear-view imagery, which helps mitigate occlusions in highly varied terrain, thereby offering rich information for building extraction. To improve the integrity of the edges of the building extraction results, this paper proposes a dual-task network (Edge-UNet, EUnet) based on UNet, incorporating an edge extraction branch to emphasize edge information while predicting building targets. We evaluate this method using a self-made GF-7 Building Dataset, the Wuhan University (WHU) Building Dataset, and the Massachusetts Buildings Dataset. Comparative analysis with other mainstream semantic segmentation networks reveals significantly higher F1 scores for the extraction results of our method. Our method exhibits superior completeness and accuracy in building edge extraction compared to unmodified algorithms, demonstrating robust performance.
Land Surface Longwave Radiation Retrieval from ASTER Clear-Sky Observations
Authors: Jiao Zhonghu , Fan Xiwei
Published: July 2024

Surface longwave radiation (SLR) plays a pivotal role in the Earth’s energy balance, influencing a range of environmental processes and climate dynamics. As the demand for high spatial resolution remote sensing products grows, there is an increasing need for accurate SLR retrieval with enhanced spatial detail. This study focuses on the development and validation of models to estimate SLR using measurements from the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) sensor. Given the limitations posed by fewer spectral bands and data products in ASTER compared to moderate-resolution sensors, the proposed approach combines an atmospheric radiative transfer model MODerate resolution atmospheric TRANsmission (MODTRAN) with the Light Gradient Boosting Machine algorithm to estimate SLR. The MODTRAN simulations were performed to construct a representative training dataset based on comprehensive global atmospheric profiles and surface emissivity spectra data. Global sensitivity analyses reveal that key inputs influencing the accuracy of SLR retrievals should reflect surface thermal radiative signals and near-surface atmospheric conditions. Validated against ground-based measurements, surface upward longwave radiation (SULR) and surface downward longwave radiation (SDLR) using ASTER thermal infrared bands and surface elevation estimations resulted in root mean square errors of 17.76 W/m2 and 25.36 W/m2, with biases of 3.42 W/m2 and 3.92 W/m2, respectively. Retrievals show systematic biases related to extreme temperature and moisture conditions, e.g., causing overestimation of SULR in hot humid conditions and underestimation of SDLR in arid conditions. While challenges persist, particularly in addressing atmospheric variables and cloud masking, this work lays a foundation for accurate SLR retrieval from high spatial resolution sensors like ASTER. The potential applications extend to upcoming satellite missions, such as the Landsat Next, and contribute to advancing high-resolution remote sensing capabilities for an improved understanding of Earth’s energy dynamics.
Dynamic Monitoring of Poyang Lake Water Area and Storage Changes from 2002 to 2022 via Remote Sensing and Satellite Gravimetry Techniques
Authors: Wang Fengwei , Zhou Qing , Gao Haipeng , Wen Yanlin , Zhou Shijian
Published: July 2024

The monitoring of Poyang Lake water area and storage changes using remote sensing and satellite gravimetry techniques is valuable for maintaining regional water resource security and addressing the challenges of global climate change. In this study, remote sensing datasets from Landsat images (Landsat 5, 7, 8 and 9) and three Gravity Recovery and Climate Experiment (GRACE) and Gravity Follow-on (GRACE-FO) mascon solutions were jointly used to evaluate the water area and storage changes in response to global and regional climate changes. The results showed that seasonal characteristics existed in the terrestrial water storage (TWS) and water area changes of Poyang Lake, with nearly no significant long-term trend, for the period from April 2002 to December 2022. Poyang Lake exhibited the largest water area in June and July every year and then demonstrated a downward trend, with relatively smaller water areas in January and November, confirmed by the estimated TWS changes. For the flood (August 2010) and drought (September 2022) events, the water area changes are 3032 km2 and 813.18 km2, with those estimated TWS changes 17.37 cm and −17.46 cm, respectively. The maximum and minimum Poyang Lake area differences exceeded 2700 km2. The estimated terrestrial water storage changes in Poyang Lake derived from the three GRACE/GRACE-FO mascon solutions agreed well, with all correlation coefficients higher than 0.92. There was a significant positive correlation higher than 0.75 between the area and TWS changes derived from the two independent monitoring techniques. Therefore, it is reasonable to conclude that combined remote sensing with satellite gravimetric techniques can better interpret the response of Poyang Lake to climate change from the aspects of water area and TWS changes more efficiently.