bluuebunny/crossref_metadata_2025 huggingface dataset:
Intracranial Hemorrhage After Reperfusion Therapies in Acute Ischemic Stroke Patients
Authors: Maïer Benjamin , Desilles Jean Philippe , Mazighi Mikael
Published: December 2020

Reperfusion therapies are the mainstay of acute ischemic stroke (AIS) treatments and overall improve functional outcome. Among the established complications of intravenous (IV) tissue-type plasminogen activator (tPA), intracranial hemorrhage (ICH) is by far the most feared and has been extensively described by seminal works over the last two decades. Indeed, IV tPA is associated with increased odds of any ICH and symptomatic ICH responsible for increased mortality rate during the first week after an AIS. Despite these results, IV tPA has been found beneficial in several pioneering randomized trials and improves functional outcome at 3 months. Endovascular therapy (EVT) combined with IV tPA for AIS patients consecutive to an anterior circulation large-vessel occlusion does not increase ICH occurrence. Of note, EVT following IV tPA leads to significantly higher rates of early reperfusion than with IV tPA alone, with no difference in ICH, which challenges the paradigm of reperfusion as a major prognostic factor for ICH complications. However, several blood biomarkers (glycemia, platelet and neutrophil count), clinical factors (age, AIS severity, blood pressure management, diabetes mellitus), and neuroradiological factors (cerebral microbleeds, infarct size) have been identified as risk factors for ICH after reperfusion therapy. In the years to come, the ultimate goal will be to further improve either reperfusion rates and functional outcome, while reducing hemorrhagic complications. To this end, various approaches being investigated are discussed in this review, such as blood-pressure control after reperfusion or the use of new antiplatelet agents as an adjunct to IV tPA and exhibit reduced hemorrhagic potential during the early phase of AIS.
Commonalities and Differences in NREM Parasomnias and Sleep-Related Epilepsy: Is There a Continuum Between the Two Conditions?
Authors: Mutti Carlotta , Bernabè Giorgia , Barozzi Noemi , Ciliento Rosario , Trippi Irene , Pedrazzi Giuseppe , Azzi Nicoletta , Parrino Liborio
Published: December 2020

Introduction: Differential diagnosis between disorders of arousal (DoA) and sleep-related hypermotor epilepsy (SHE) often represents a clinical challenge. The two conditions may be indistinguishable from a semiological point of view and the scalp video-polysomnography is often uninformative. Both disorders are associated with variable hypermotor manifestations ranging from major events to fragments of a hierarchical continuum of increasing intensity, complexity, and duration. Given their semiological overlap we decided to explore the sleep texture of DoA and SHE seeking for similarities and differences.

Methods: We analyzed sleep macrostructure and CAP (cyclic alternating pattern) parameters in a cohort of 35 adult DoA patients, 40 SHE patients and 24 healthy sleepers, all recorded and scored in the same sleep laboratory. Nocturnal behavioral manifestations included minor motor events, paroxysmal arousals and major attacks in SHE, and simple, rising, or complex arousal movements in DoA.

Results: Compared to healthy controls, DoA and SHE showed similar amounts of sleep efficiency, light sleep, deep sleep, REM sleep, CAP subtypes. Both groups also showed slow wave sleep fragmentation and an increased representation of stage N3 in the second part of the night. The only discriminating elements between the two conditions regarded sleep length (more reduced in DoA) and sleep instability (more elevated in SHE). In DoA recordings, all motor episodes arose from NREM sleep: 37% during light NREM stages and 63% during stage N3 (simple arousal movements: 94%). In SHE recordings, 57% of major attacks occurred during stage N3.

Conclusions: So far, emphasis has been placed on the differentiation of sleep-related epilepsy and NREM arousal disorders. However, the impressive analogies between DoA and SHE suggest the existence of an underestimated continuum across the conditions, linked by increased levels of sleep instability, higher amounts of slow wave sleep and NREM/REM sleep imbalance. Sleep texture is extremely similar in the two conditions, although CAP metrics disclose quantitative differences. In particular, SHE patients show a higher arousal instability compared to DoA subjects. Given their clinical and epidemiological overlap, a common genetic background is also hypothesized. In such a perspective, we suggest that the consolidated dichotomy DoA vs. SHE should be reappraised.
Using Posterior EEG Theta Band to Assess the Effects of Architectural Designs on Landmark Recognition in an Urban Setting
Authors: Rounds James D. , Cruz-Garza Jesus Gabriel , Kalantari Saleh
Published: December 2020

The process of urban landmark-based navigation has proven to be difficult to study in a rigorous fashion, primarily due to confounding variables and the problem of obtaining reliable data in real-world contexts. The development of high-resolution, immersive virtual reality technologies has opened exciting new possibilities for gathering data on human wayfinding that could not otherwise be readily obtained. We developed a research platform using a virtual environment and electroencephalography (EEG) to better understand the neural processes associated with landmark usage and recognition during urban navigation tasks. By adjusting the architectural parameters of different buildings in this virtual environment, we isolated and tested specific design features to determine whether or not they served as a target for landmarking. EEG theta band (4–7 Hz) event-related synchronization/desynchronization over posterior scalp areas was evaluated at the time when participants observed each target building along a predetermined self-paced route. A multi-level linear model was used to investigate the effects of salient architectural features on posterior scalp areas. Our results support the conclusion that highly salient architectural features—those that contrast sharply with the surrounding environment—are more likely to attract visual attention, remain in short-term memory, and activate brain regions associated with wayfinding compared with non-salient buildings. After establishing this main aggregate effect, we evaluated specific salient architectural features and neural correlates of navigation processing. The buildings that most strongly associated extended gaze time, location recall accuracy, and changes in theta-band neural patterns with landmarking in our study were those that incorporated rotational twist designs and natural elements such as trees and gardens. Other building features, such as unusual façade patterns or building heights, were to a lesser extent also associated with landmarking.
Effects of Transcranial Direct Current Stimulation on Motor Function in Children 8–12 Years With Developmental Coordination Disorder: A Randomized Controlled Trial
Authors: Grohs Melody N. , Craig Brandon T. , Kirton Adam , Dewey Deborah
Published: December 2020

Background and objectives: Developmental coordination disorder (DCD) is a neurodevelopmental motor disorder occurring in 5-6% of school-aged children. It is suggested that children with DCD show deficits in motor learning. Transcranial direct current stimulation (tDCS) enhances motor learning in adults and children but is unstudied in DCD. We aimed to investigate if tDCS, paired with motor skill training, facilitates motor learning in a pediatric sample with DCD.

Methods: Twenty-eight children with diagnosed DCD (22 males, mean age: 10.62 ± 1.44 years) were randomized and placed into a treatment or sham group. Anodal tDCS was applied (1 mA, 20 min) in conjunction with fine manual training over 5 consecutive days. Children's motor functioning was assessed with the Purdue Pegboard Test and Jebsen-Taylor Hand Function Test at baseline, post-intervention and 6 weeks following intervention. Group differences in rates of motor learning and skill transfer/retention were examined using linear mixed modeling and repeated measures ANOVAs, respectively.

Results: There were no serious adverse events or drop-outs and procedures were well-tolerated. Independent of group, all participants demonstrated improved motor scores over the 5 training days [F(69.280), p < 0.001, 95% CI (0.152, 0.376)], with no skill decay observed at retention. There was no interaction between intervention group and day [F(2.998), p = 0.086, 95% CI (−0.020, 0.297)].

Conclusion: Children with DCD demonstrate motor learning with long-term retention of acquired skill. Motor cortex tDCS did not enhance motor learning as seen in other populations. Before conclusions of tDCS efficacy can be drawn, additional carefully designed trials with reproducible results are required.

Clinical Trial Registration:ClinicalTrials.gov: NCT03453983
Long-Term Soy Protein Isolate Consumption Reduces Liver Steatosis Through Changes in Global Transcriptomics in Obese Zucker Rats
Authors: Kozaczek Melisa , Bottje Walter , Kong Byungwhi , Dridi Sami , Albataineh Diyana , Lassiter Kentu , Hakkak Reza
Published: December 2020

To determine how soy protein isolate (SPI) ameliorated liver steatosis in male obese Zucker rats, we conducted global transcriptomic expression (RNAseq) analysis on liver samples of male rats fed either the SPI or a control casein (CAS)-based diet (n = 8 per group) for 16 weeks. Liver transcriptomics were analyzed using an Ilumina HiSeq system with 2 × 100 base pair paired-end reads method. Bioinformatics was conducted using Ingenuity Pathway Analysis (IPA) software (Qiagen, CA) with P < 0.05 and 1.3-fold differential expression cutoff values. Regression analysis between RNAseq data and targeted mRNA expression analysis of 12 top differentially expressed genes (from the IPA program) using quantitative PCR (qPCR) revealed a significant regression analysis (r2 = 0.69, P = 0.0008). In addition, all qPCR values had qualitatively similar direction of up- or down-regulation compared to the RNAseq transcriptomic data. Diseases and function analyses that were based on differentially expressed target molecules in the dataset predicted that lipid metabolism would be enhanced whereas inflammation was predicted to be inhibited in SPI-fed compared to CAS-fed rats at 16 weeks. Combining upstream regulator and regulator effects functions in IPA facilitates the prediction of upstream regulators (e.g., transcription regulators) that could play important roles in attenuating or promoting liver steatosis due to SPI or CAS diets. Upstream regulators that were predicted to be activated (from expression of down-stream targets) linked to increased conversion of lipid and transport of lipid in SPI-fed rats included hepatocyte nuclear factor 4 alpha (HNF4A) and aryl hydrocarbon receptor (AHR). Upstream regulators that were predicted to be activated in CAS-fed rats linked to activation of phagocytosis and neutrophil chemotaxis included colony stimulating factor 2 and tumor necrosis factor. The results provide clear indication that long-term SPI-fed rats exhibited diminished inflammatory response and increased lipid transport in liver compared to CAS-fed rats that likely would contribute to reduced liver steatosis in this obese Zucker rat model.
SARS-CoV-2 Spike-Heat Shock Protein A5 (GRP78) Recognition may be Related to the Immersed Human Coronaviruses
Authors: Elfiky Abdo A.
Published: December 2020

The human coronavirus (HCoV), SARS-CoV-2, caused more than 34 M confirmed infections from which more than 1 M deaths are reported until now (the WHO situation report-154). The current pandemic causes severe socio-economic burden. Due to the importance of understanding of the mode of recognition and viral entry, spike protein shed drug designers as the first look protein target with the first released solved structure on 26 February 2020 (PDB ID: 6VSB). It is proposed that the recognition site for GRP78 is found in SARS-CoV-2 and the immersed human coronaviruses but experimental validation is still required.
Hydroxysafflor Yellow A: A Systematical Review on Botanical Resources, Physicochemical Properties, Drug Delivery System, Pharmacokinetics, and Pharmacological Effects
Authors: Zhao Feng , Wang Ping , Jiao Yuanyuan , Zhang Xiaoxiao , Chen Daquan , Xu Haiyu
Published: December 2020

Hydroxysafflower yellow A (HSYA), as a principal natural ingredient extracted from safflower (Carthamus tinctorius L.), has significant pharmacological activities, such as antioxidant, anti-inflammatory, anticoagulant, and anticancer effects. However, chemical instability and low bioavailability have been severely hampering the clinical applications of HSYA during the treatment of cardiovascular and cerebrovascular disease. Therefore, this present review systematically summarized the materials about HSYA, including acquisition methods, extraction and detection methods, pharmacokinetics, pharmacological effects and molecular mechanism, especially focus on the possible causes and resolutions about the chemical instability and low bioavailability of HSYA, in order to provide relatively comprehensive basic data for the related research of HSYA.
iTRAQ-Based Proteomic Analysis Reveals the Role of the Biological Control Agent, Sinorhizobium fredii Strain Sneb183, in Enhancing Soybean Resistance Against the Soybean Cyst Nematode
Authors: Wang Yuanyuan , Yang Ruowei , Feng Yaxing , Sikandar Aatika , Zhu Xiaofeng , Fan Haiyan , Liu Xiaoyu , Chen Lijie , Duan Yuxi
Published: December 2020

The soybean cyst nematode (SCN), Heterodera glycines Ichinohe, poses a serious threat to soybean production worldwide. Biological control agents have become eco-friendly candidates to control pathogens. Our previous study indicated that the biocontrol agent, Sinorhizobium fredii strain Sneb183, may induce soybean resistance to SCN. To study the mechanisms underlying induced disease resistance in the plant by Sneb183, an iTRAQ (isobaric tag for relative and absolute quantitation)-based proteomics approach was used to identify proteomic changes in SCN-infected soybean roots derived from seeds coated with the Sneb183 fermentation broth or water. Among a total of 456 identified differentially expressed proteins, 212 and 244 proteins were upregulated and downregulated, respectively, in Sneb183 treated samples in comparison to control samples. Some identified differentially expressed proteins are likely to be involved in the biosynthesis of phenylpropanoid, flavone, flavanol, and isoflavonoid and have a role in disease resistance and adaptation to environmental stresses. We used quantitative real-time PCR (qRT-PCR) to analyze key genes, including GmPAL (phenylalanine ammonia-lyase), GmCHR (chalcone reductase), GmCHS (chalcone synthase), and GmIFS (isoflavone synthase), that are involved in isoflavonoid biosynthesis in Sneb183-treated and control samples. The results showed that these targeted genes have higher expression levels in Sneb183-treated than in control samples. High performance liquid chromatography (HPLC) analysis further showed that the contents of daidzein in Sneb183-treated samples were 7.24 times higher than those in control samples. These results suggested that the Sinorhizobium fredii strain Sneb183 may have a role in inducing isoflavonoid biosynthesis, thereby resulting in enhanced resistance to SCN infection in soybean.
Organizational Justice, Professional Identification, Empathy, and Meaningful Work During COVID-19 Pandemic: Are They Burnout Protectors in Physicians and Nurses?
Authors: Correia Isabel , Almeida Andreia E.
Published: December 2020

Burnout has been recognized as a serious health problem. In Portugal, before COVID-19 Pandemic, there were strong indicators of high prevalence of burnout in physicians and nurses. However, the Portuguese Health Care Service was able to efficiently respond to the increased demands. This study intends to understand how psychosocial variables might have been protective factors for burnout in physicians and nurses in Portugal. Specifically, we considered several psychosocial variables that have been found to be protective factors for burnout in previous research and we compared their predictive and unique impact in the prediction of burnout. These variables are perceptions of justice (distributive, procedural, justice from colleagues, justice from patients, and their families), professional identification, meaningful work and empathy. We also included workload, as a risk factor, and controlled other variables that can be confounds for burnout, such as socio-demographic variables, ideological variables (religiosity, political orientation), and specific variables related with COVID-19 pandemic. The sample of the present study is composed by 229 physicians (aged between 23 and 70 years old,M= 36.54;SD= 10.72; 48% male and 52% female) and 268 nurses (aged between 22 and 69 years old,M= 34.96;SD= 9.52; 27% male and 73% female). An online survey was created using Qualtrics and participants were recruited via Facebook and LinkedIn. The data were collected during 29 days (between the 45th and the 74th days after the first diagnosed case of COVID-19 in Portugal). The results showed that workload was a significant risk factor, except for disengagement in physicians. The most consistent protectors across samples were procedural justice (for both dimensions of burnout, both in physicians and nurses) and professional identification (for disengagement, both in physicians and nurses; for exhaustion only in physicians). This study suggests that decreasing workload and promoting procedural justice and professional identification are key factors that might be simultaneously and independently addressed in interventions for reducing the risk of burnout or preventing it from occurring in the first place.
Psychological Impact of the COVID-19 Outbreak on Nurses in China: A Nationwide Survey During the Outbreak
Authors: Liu Yan , Long Youlin , Cheng Yifan , Guo Qiong , Yang Liu , Lin Yifei , Cao Yu , Ye Lei , Jiang Yan , Li Ka , Tian Kun , A Xiaoming , Sun Cheng , Zhang Fang , Song Xiaoxia , Liao Ga , Huang Jin , Du Liang
Published: December 2020

Background: The COVID-19 pandemic is a major public health issue and challenge to health professionals. In similar epidemics, nurses experienced more distress than other providers.

Methods: We surveyed both on-duty nurses caring for infected patients and second-line nurses caring for uninfected patients from Hubei and other provinces throughout China.

Results: We received completed surveys from 1,364 nurses from 22 provinces: 658 front-line and 706 second-line nurses. The median (IQR) GHQ-28 score of all nurses was 17 (IQR 11–24). The overall incidence of mild-to-moderate distress (GHQ score > 5) was 28%; that for severe distress (GHQ score > 11) was 6%. The incidence of mild-to-moderate distress in the second-line nurses was higher than that in the front-line nurses (31 vs. 25%; OR, 0.74; 95 CI, 0.58–0.94). Living alone (OR, 0.62; 95% CI, 0.44–0.86) and feeling supported (OR, 0.82, 95% CI, 0.74–0.90) independently predicted lower anxiety.

Conclusions: During the COVID-19 pandemic, the psychological problems of all nurses were generally serious. The interviewed second-line nurses face more serious issues than the front-line nurses.
Non-wetting Materials
Authors: Frumkin Valeri , Bercovici Moran
Published: December 2020

A drop of rain falling on a surface, such as the car’s window or the palm of your hand, tends to spread over the surface and even hang onto it. But there are materials, such as the leaf of the lotus flower, from which a drop of water will roll down to the ground as an almost perfect sphere, while cleaning the material of dust particles and other pollutions. In this article, we will investigate what influences the tendency of different materials to be wetted to different extents, and how can we design and produce such materials in the lab.
Dynamics of Gastro-Intestinal Strongyle Parasites in a Group of Translocated, Wild-Captured Asiatic Wild Asses in Kazakhstan
Authors: Gliga Diana S. , Petrova Natalia , Linnell John D. C. , Salemgareyev Albert R. , Zuther Steffen , Walzer Chris , Kaczensky Petra
Published: December 2020

Asiatic wild ass (Kulan, Equus hemionus) population range and numbers became severely reduced and a reintroduction project is currently aiming to re-establish a population in the Central Steppe of Kazakhstan. Pre-emptive deworming is often recommended for equid translocations but eliminating parasites prior to translocation could cause disruptions in a balanced host-parasite relationship, adding an additional stressor to an already stressful intervention involving capture, transport, and adaptation to a new environment. Following a disease risk assessment, we decided against pre-emptive deworming and focused on monitoring the first group of nine translocated kulan in a large acclimatization enclosure prior to release. Over the 5-month acclimatization period, we regularly collected fecal samples and analyzed the shedding intensity of gastro-intestinal parasite eggs, obtained time budgets through behavioral observations, and visually assessed body condition. We identified strongyles (Strongylinae and Cyathostominae) and pinworms (Oxyuris equi) in fecal samples. All individuals shed strongyle eggs and two of the nine individuals had higher shedding intensities, but rarely reached levels for which deworming is recommended. All kulan appeared healthy throughout the observation period, aggressive interactions were very rare, and time budgets were very similar and dominated by feeding. Our results suggest that in translocation projects where the risk of introducing new parasites is minimal, pre-emptive treatment in wild equids can be replaced with non-invasive monitoring during the acclimatization period. We acknowledge that the small number of kulan, the large size of the enclosure, and the low temperatures during the animals stay in the acclimatization enclosure may all have reduced infestation pressure.
Effect of Alternative Palatal Root Access Technique on Fracture Resistance of Root Canal Treated Maxillary Fourth Premolar Teeth in Dogs
Authors: Matelski Jennifer , Rendahl Aaron , Goldschmidt Stephanie
Published: December 2020

A biomechanical study was performed to identify the effect of different treatment methods for difficult to instrument palatal roots on the fracture resistance of root canal treated maxillary fourth premolar teeth in dogs. Forty maxillary fourth premolar teeth with surrounding alveolar bone were harvested from beagle cadavers. Inclusion criteria included maxillary fourth premolars with no evidence of disease and similar distal root canal volumes on radiographic evaluation. The teeth were randomly divided into a control group and three treatment groups based on the endodontic treatment technique for the palatal root. The control group had a single 2 mm transcoronal access on the mesiobuccal aspect of the tooth to allow instrumentation of both the mesiobuccal and palatal root through a single small access. Alternative treatment modalities that are described for difficult to instrument palatal roots investigated in this study included enlarging the transcoronal mesiobuccal access to 4 mm, making an additional access directly over the palatal root (2 mm), and hemisection with extraction of the palatal root. All teeth had the same distal root access size (2 mm) and relative location. After access, all teeth were filed, shaped, obturated, and restored in the same fashion. Axial compression testing was performed at an angle of 60 degrees to the long axis of the tooth using a universal materials testing machine. The maximum force prior to fracture was determined for each tooth based on a force vs. deflection curve. The mean maximum force prior to fracture for all teeth was 831 N. No significant difference in mean fracture resistance was identified between the control group and treatment groups or between the different treatment groups themselves. Thus, when faced with a difficult to instrument palatal root, the treatment method chosen should be based on operator preference and experience.
Investigation of Pig Activity Based on Video Data and Semi-Supervised Neural Networks
Authors: Wutke Martin , Schmitt Armin Otto , Traulsen Imke , Gültas Mehmet
Published: December 2020

The activity level of pigs is an important stress indicator which can be associated to tail-biting, a major issue for animal welfare of domestic pigs in conventional housing systems. Although the consideration of the animal activity could be essential to detect tail-biting before an outbreak occurs, it is often manually assessed and therefore labor intense, cost intensive and impracticable on a commercial scale. Recent advances of semi- and unsupervised convolutional neural networks (CNNs) have made them to the state of art technology for detecting anomalous behavior patterns in a variety of complex scene environments. In this study we apply such a CNN for anomaly detection to identify varying levels of activity in a multi-pen problem setup. By applying a two-stage approach we first trained the CNN to detect anomalies in the form of extreme activity behavior. Second, we trained a classifier to categorize the detected anomaly scores by learning the potential activity range of each pen. We evaluated our framework by analyzing 82 manually rated videos and achieved a success rate of 91%. Furthermore, we compared our model with a motion history image (MHI) approach and a binary image approach using two benchmark data sets, i.e., the well established pedestrian data sets published by the University of California, San Diego (UCSD) and our pig data set. The results show the effectiveness of our framework, which can be applied without the need of a labor intense manual annotation process and can be utilized for the assessment of the pig activity in a variety of applications like early warning systems to detect changes in the state of health.
Frequency of Occurrence and Ingested Biomass of Different Prey of the Barn Owl Tyto alba in an Island Ecosystem
Authors: Viganò Miriam , Ancillotto Leonardo , Agnelli Paolo , Ficetola Gentile Francesco , Mori Emiliano
Published: December 2020

The Barn Owl Tyto alba is commonly reported as a non-selective predator of small mammals, and its diet has been thoroughly analyzed also to assess the small mammal assembly composition in many study areas. The aim of this work was to analyze the diet of the Barn Owl in the Elba island through the analysis of 161 pellets collected in September 2020. Undigested fragments were isolated and compared with reference collections. We confirmed that the Barn Owl is a typical predator of field mice (62% of relative frequency), with synanthropic murid rodents as the second category of prey. The frequency of consumption of shrews increased by 9% with respect to the previous work, suggesting that the natural environment of Elba island is still in a good health status. Moreover, fragments of two newborn hares were detected, increasing the knowledge on the local trophic spectrum of the Barn Owl. Finally, the skull of a Geoffroy’s Myotis Myotis emarginatus confirmed the presence of this species in Elba island after over 60 years from the first unconfirmed record. Repeated studies conducted in the same study site may provide useful information on prey population trends and local environmental status.
Carbon Allotropes as ITO Electrode Replacement Materials in Liquid Crystal Devices
Authors: Dierking Ingo
Published: December 2020

Indium tin oxide (ITO)-free optoelectronic devices have been discussed for a number of years in the light of a possible indium shortage as demand rises. In particular, this is due to the largely increased number of flat panel displays and especially liquid crystal displays (LCDs) being produced for home entertainment TV and mobile technologies. While a shortage of primary indium seems far on the horizon, nevertheless, recycling has become an important issue, as has the development of ITO-free electrode materials, especially for flexible liquid crystal devices. The main contenders for new electrode technologies are discussed with an emphasis placed on carbon-based materials for LCDs, including composite approaches. At present, these already fulfil the technical specifications demanded from ITO with respect to transmittance and sheet resistance, albeit not in relation to cost and large-scale production. Advantages and disadvantages of ITO-free technologies are discussed, with application examples given. An outlook into the future suggests no immediate transition to carbon-based electrodes in the area of LCDs, while this may change in the future once flexible displays and environmentally friendly smart window solutions or energy harvesting building coverings become available.
Waste Wash-Water Recycling in Ready Mix Concrete Plants
Authors: Ghrair Ayoup M. , Heath Andrew , Paine Kevin , Al Kronz Muath
Published: December 2020

The management of waste wash-water (WWW) is one of the most significant environmental problems associated with ready-mix concrete production worldwide. The problems are exacerbated should it be disposed of in an inappropriate manner. This study evaluated the potential of WWW recycling in ready mix concrete plants in Jordan. A representative waste wash-water sample (400 L) was collected from a basin in a ready-mix concrete company. A pilot plant on the lab scale was fabricated and installed. The treatment system consisted of a concrete washout reclaimer, wedgebed slurry settling pond, slow sand filtration unit, and a neutralization unit. Water samples were collected from all stages of the pilot plant and analyzed. The collected waste wash-water samples were utilized for replacement of well water (mixing water) at various ratios. Fourteen concrete mixtures were produced and cast, as well as tested at various curing ages (7, 28, and 90 days). The results show that the raw WWW was not acceptable as mixing water even after dilution as it led to significant reductions in concrete compressive strength and low workability. However, the WWW from the settling pond, the filtered WWW and the filtered-neutralized WWW at dilution ratios up to 75% were shown to be potential alternatives to fresh water for ready-mixed concrete. Therefore, the current guidelines for mixing water quality should be revised to encourage the reuse of the WWW.
Multiresidue Analysis of Organic UV Filters and UV Stabilizers in Fish of Common Consumption
Authors: Gimeno-Monforte Sandra , Montesdeoca-Esponda Sarah , Sosa-Ferrera Zoraida , Santana-Rodríguez José Juan , Castro Óscar , Pocurull Eva , Borrull Francesc
Published: December 2020

Fish species can bioaccumulate different pollutants present in the marine environments and incorporate them into the trophic chain. In this work, the occurrence of organic ultraviolet (UV) stabilizers and filters in different species of fishes of high consumption has been studied. A multiresidue method based on microwave-assisted extraction and ultra-high performance liquid chromatography with mass spectrometry detection was developed and then it was applied to nine fish species from markets in the Canary Islands and Catalonia (Spain). Three UV filters (BP-3, OC and BM-DBM) and two stabilizers (UV-328 and UV-329) were found in some of the studied species, in concentrations ranging between 0.067 and 0.683 µg g−1 dry weight (dw). BP-3 (UV filter) was the most frequently detected compound, followed by UV-329 (UV stabilizer). Thunnus thynnus was the most heavily polluted species, with a concentration of 1.201 µg g−1 dw as the sum of all measured compounds.
Evaluation of Volatilomic Fingerprint from Apple Fruits to Ciders: A Useful Tool to Find Putative Biomarkers for Each Apple Variety
Authors: Medina Sonia , Perestrelo Rosa , Pereira Regina , Câmara José S.
Published: December 2020

Aroma is a crucial criterion to assess the quality of apple fruits, juices, and ciders. The aim of this study was to explore similarities and differences in volatile profiles among apple fruits, juices, and ciders from different apple varieties (Festa, Branco, and Domingos) by headspace solid-phase microextraction gas chromatography–mass spectroscopy (HS–SPME/GC–MS). A total of 142 volatile organic compounds (VOCs) were identified, but only 9 were common in all analysed matrices and apple-tested varieties. Esters, alcohols, and aldehydes presented a higher concentration in apple fruits and juices, whereas esters, alcohols, and acids were dominant in ciders. Moreover, there were unique VOCs for each matrix and for each variety, highlighting the importance of the selection of apple varieties as an important factor to obtain good sensory and quality ciders, multiple benefits, and legal protection against the misuse of local products.
Production of a Recombinant Single-Domain Antibody for Gluten Detection in Foods Using the Pichia pastoris Expression System
Authors: García-García Aina , Madrid Raquel , Garcia-Calvo Eduardo , Mendoza-Chamizo Belén , García Teresa , Martin Rosario
Published: December 2020

The detection of gluten in foodstuffs has become a growing concern in food allergen management as a result of the high ratio of population sensitive to the main gluten-containing cereals. In this study, a promising single-domain antibody previously isolated by phage display (dAb8E) was produced in Pichia pastoris resulting in high levels of the antibody fragment expression (330 mg/L). The purified dAb8E was proved to specifically bind to gluten proteins from wheat, barley and rye, exhibiting no cross reaction to other heterologous species. The dynamic range of the sandwich enzyme-linked immunosorbent assay (ELISA) covered 0.1 to 10 µg/mL of gliadin, reaching a limit of detection of 0.12 µg/mL. When experimental binary mixtures of the target cereals were analyzed, the limit of detection was 0.13 mg/g, which would theoretically correspond to gluten concentrations of approximately 13 mg/kg. Finally, thirty commercially available food products were analyzed by means of the developed assay to further confirm the applicability of the dAb8E for gluten determination. The proposed methodology enabled the generation of a new gluten-specific nanobody which could be used to guarantee the appropriate labelling of gluten-free foods.
Comprehensive Profiling of the Native and Modified Peptidomes of Raw Bovine Milk and Processed Milk Products
Authors: Wölk Michele , Milkovska-Stamenova Sanja , Hoffmann Ralf
Published: December 2020

Bovine milk contains a variety of endogenous peptides, partially formed by milk proteases that may exert diverse bioactive functions. Milk storage allows further protease activities altering the milk peptidome, while processing, e.g., heat treatment can trigger diverse chemical reactions, such as Maillard reactions and oxidations, leading to different posttranslational modifications (PTMs). The influence of processing on the native and modified peptidome was studied by analyzing peptides extracted from raw milk (RM), ultra-high temperature (UHT) milk, and powdered infant formula (IF) by nano reversed-phase liquid chromatography coupled online to electrospray ionization (ESI) tandem mass spectrometry. Only unmodified peptides proposed by two independent software tools were considered as identified. Thus, 801 identified peptides mainly originated from αS- and β-caseins, but also from milk fat globular membrane proteins, such as glycosylation-dependent cell adhesion molecule 1. RM and UHT milk showed comparable unmodified peptide profiles, whereas IF differed mainly due to a higher number of β-casein peptides. When 26 non-enzymatic posttranslational modifications (PTMs) were targeted in the milk peptidomes, 175 modified peptides were identified, i.e., mostly lactosylated and a few hexosylated or oxidized peptides. Most modified peptides originated from αS-caseins. The numbers of lactosylated peptides increased with harsher processing.
Interferometer Sensing and Control for the Advanced Virgo Experiment in the O3 Scientific Run
Authors: Allocca Annalisa , Bersanetti Diego , Casanueva Diaz Julia , De Rossi Camilla , Mantovani Maddalena , Masserot Alain , Rolland Loïc , Ruggi Paolo , Swinkels Bas , Tapia San Martin Enzo Nicolas , Vardaro Marco , Was Michal
Published: December 2020

Advanced Virgo is a 2nd-generation laser interferometer based in Cascina (Italy) aimed at the detection of gravitational waves (GW) from astrophysical sources. Together with the two USA-based LIGO interferometers they constitute a network which operates in coincidence. The three detectors observed the sky simultaneously during the last part of the second Observing Run (O2) in August 2017, and this led to two paramount discoveries: the first three-detector observation of gravitational waves emitted from the coalescence of a binary black hole system (GW170814), and the first detection ever of gravitational waves emitted from the coalescence of a binary neutron star system (GW170817). Coincident data taking was re-started for the third Observing Run (O3), which started on 1st April 2019 and lasted almost one year. This paper will describe the new techniques implemented for the longitudinal controls with respect to the ones already in use during O2. Then, it will present an extensive description of the full scheme of the angular controls of the interferometer, focusing on the different control strategies that are in place in the different stages of the lock acquisition procedure, which is the complex sequence of operations by which an uncontrolled, “free” laser interferometer is brought to the final working point, which allows the detector to reach the best sensitivity.
Fine-Tuning the Optical Design of the Advanced Virgo+ Gravitational-Wave Detector Using Binary-Neutron Star Signals
Authors: Baird Jonathon , Barsuglia Matteo
Published: December 2020

Advanced Virgo+ is a major upgrade of the Advanced Virgo gravitational-wave detector aiming to increase sensitivity in terms of binary neutron star (BNS) range by a factor 3–5 in the next few years. In this work, we present an optimization of the mirror transmittances for the second phase of the project (to be implemented for the O5 observation run) using a random walk algorithm implemented with the advGWINC software. In addition to BNS range, a post merger (PM) SNR is also used as a figure of merit to identify configurations that fine-tune the sensitivity curve, as a function of arm-cavity round trip losses.
Development of a Frequency Tunable Green Laser Source for Advanced Virgo+ Gravitational Waves Detector
Authors: De Rossi Camilla , Brooks Jonathan , Casanueva Diaz Julia , Chiummo Antonino , Genin Eric , Gosselin Matthieu , Leroy Nicolas , Mantovani Maddalena , Montanari Beatrice , Nocera Flavio , Pillant Gabriel
Published: December 2020

After one year of data taking, the observing run three (O3), we are preparing for an improved version of the system, named the Advanced Virgo plus. One of the major upgrades will be the installation of the signal recycling mirror to form an additional optical cavity and improve the sensitivity of the interferometer. This also requires a change in the lock acquisition strategy. In particular, the arms will be locked at the beginning with lasers at a different wavelength from the main one. Such a strategy has already been implemented and tested in LIGO and KAGRA, and in this paper we will present how it has been conceived in Virgo.
Activation of the Ion Channel TRPV4 Induces Epithelial to Mesenchymal Transition in Breast Cancer Cells
Authors: Azimi Iman , Robitaille Mélanie , Armitage Kaela , So Choon Leng , Milevskiy Michael J. G. , Northwood Korinne , Lim Huai Fang , Thompson Erik W. , Roberts-Thomson Sarah J. , Monteith Gregory R.
Published: December 2020

Epithelial to mesenchymal transition (EMT) in cancer is important in therapeutic resistance and invasiveness. Calcium signaling is key to the induction of EMT in breast cancer cells. Although inhibition of specific calcium-permeable ion channels regulates the induction of a sub-set of EMT markers in breast cancer cells, it is still unclear if activation of a specific calcium channel can be a driver for the induction of EMT events. In this study, we exploited the availability of a selective pharmacological activator of the calcium-permeable ion channel TRPV4 to assess the direct role of calcium influx in EMT marker induction. Gene association studies revealed a link between TRPV4 and gene-ontologies associated with EMT and poorer relapse-free survival in lymph node-positive basal breast cancers. TRPV4 was an important component of the calcium influx phase induced in MDA-MB-468 breast cancer cells by the EMT inducer epidermal growth factor (EGF). Pharmacological activation of TRPV4 then drove the induction of a variety of EMT markers in breast cancer cells. These studies demonstrate that calcium influx through specific pathways appears to be sufficient to trigger EMT events.
Involvement of the FAK Network in Pathologies Related to Altered Mechanotransduction
Authors: Urciuoli Enrica , Peruzzi Barbara
Published: December 2020

Mechanotransduction is a physiological process in which external mechanical stimulations are perceived, interpreted, and translated by cells into biochemical signals. Mechanical stimulations exerted by extracellular matrix stiffness and cell–cell contacts are continuously applied to living cells, thus representing a key pivotal trigger for cell homeostasis, survival, and function, as well as an essential factor for proper organ development and metabolism. Indeed, a deregulation of the mechanotransduction process consequent to gene mutations or altered functions of proteins involved in perceiving cellular and extracellular mechanics can lead to a broad range of diseases, from muscular dystrophies and cardiomyopathies to cancer development and metastatization. Here, we recapitulate the involvement of focal adhesion kinase (FAK) in the cellular conditions deriving from altered mechanotransduction processes.
β-Glucan Metabolic and Immunomodulatory Properties and Potential for Clinical Application
Authors: Murphy Emma J. , Rezoagli Emanuele , Major Ian , Rowan Neil J. , Laffey John G.
Published: December 2020

β-glucans are complex polysaccharides that are found in several plants and foods, including mushrooms. β-glucans display an array of potentially therapeutic properties. β-glucans have metabolic and gastro-intestinal effects, modulating the gut microbiome, altering lipid and glucose metabolism, reducing cholesterol, leading to their investigation as potential therapies for metabolic syndrome, obesity and diet regulation, gastrointestinal conditions such as irritable bowel, and to reduce cardiovascular and diabetes risk. β-glucans also have immune-modulating effects, leading to their investigation as adjuvant agents for cancers (solid and haematological malignancies), for immune-mediated conditions (e.g., allergic rhinitis, respiratory infections), and to enhance wound healing. The therapeutic potential of β-glucans is evidenced by the fact that two glucan isolates were licensed as drugs in Japan as immune-adjuvant therapy for cancer in 1980. Significant challenges exist to further clinical testing and translation of β-glucans. The diverse range of conditions for which β-glucans are in clinical testing underlines the incomplete understanding of the diverse mechanisms of action of β-glucans, a key knowledge gap. Furthermore, important differences appear to exist in the effects of apparently similar β-glucan preparations, which may be due to differences in sources and extraction procedures, another poorly understood issue. This review will describe the biology, potential mechanisms of action and key therapeutic targets being investigated in clinical trials of β-glucans and identify and discuss the key challenges to successful translation of this intriguing potential therapeutic.
Microendoscopic Posterior Decompression for Treating Thoracic Myelopathy Caused by Ossification of the Ligamentum Flavum: Case Series
Authors: Baba Satoshi , Shiboi Ryutaro , Yokosuka Jyunichi , Oshima Yasushi , Takano Yuichi , Iwai Hiroki , Inanami Hirohiko , Koga Hisashi
Published: December 2020

Background and Objectives: Ossification of the ligamentum flavum (OLF) is a relatively common cause of thoracic myelopathy. Surgical treatment is recommended for patients with myelopathy. Generally, open posterior decompression, with or without fusion, is selected to treat OLF. We performed minimally invasive posterior decompression using a microendoscope and investigated the efficacy of this approach in treating limited type of thoracic OLF. Materials and Methods: Microendoscopic posterior decompression was performed for 19 patients (15 men and four women) with thoracic OLF with myelopathy aged between 35 to 81 years (mean age, 61.9 years). Neurological examination and preoperative magnetic resonance imaging (MRI) and computed tomography (CT) were used to identify the location and morphology of OLF. The surgery was performed using a midline approach or a unilateral paramedian approach depending on whether the surgeon used a combination of a tubular retractor and endoscope. The numerical rating scale (NRS) and modified Japanese Orthopedic Association (mJOA) scores were compared pre- and postoperatively. Perioperative complications and the presence of other spine surgeries before and after thoracic OLF surgery were also investigated. Results: Four midline and 15 unilateral paramedian approaches were performed. The average operative time per level was 99 min, with minor blood loss. Nine patients had a history of cervical or lumbar spine surgery before or after thoracic spine surgery. The mean pre- and postoperative NRS scores were 6.6 and 5.3, respectively. The mean recovery rate as per the mJOA score was 33.1% (mean follow-up period, 17.8 months), the recovery rates were significantly different between patients who underwent thoracic spine surgery alone (50.5%) and patients who underwent additional spine surgeries (13.7%). Regarding adverse events, one patient experienced dural tear, another experienced postoperative hematoma, and one other underwent reoperation for adjacent thoracic stenosis. Conclusion: Microendoscopic posterior decompression was applicable in limited type of thoracic OLF surgery including beak-shaped type and multi vertebral levels. However, whole spine evaluation is important to avoid missing other combined stenoses that may affect outcomes.
Fabrication of Double Emission Enhancement Fluorescent Nanoparticles with Combined PET and AIEE Effects
Authors: Wu Hsing-Ju , Chang Cheng-Chung
Published: December 2020

The major challenge in the fabrication of fluorescent silica nanoparticles (FSNs) based on dye-doped silica nanoparticles (DDSNs) is aggregation-caused fluorescence quenching. Here, we constructed an FSN based on a double emission enhancement (DEE) platform. A thio-reactive fluorescence turn-on molecule, N-butyl-4-(4-maleimidostyryl)-1,8-naphthalimide (CS), was bound to a silane coupling agent, (3-mercaptopropyl)-trimethoxysilane (MPTMS), and the product N-butyl-4-(3-(trimethoxysilyl-propylthio)styryl)-1,8-naphthalimide (CSP) was further used to fabricate a core–shell nanoparticle through the Stöber method. We concluded that the turn-on emission by CSP originated from the photoinduced electron transfer (PET) between the maleimide moiety and the CSP core scaffold, and the second emission enhancement was attributed to the aggregation-induced emission enhancement (AIEE) in CSP when encapsulated inside a core–shell nanoparticle. Thus, FSNs could be obtained through DEE based on a combination of PET and AIEE effects. Systematic investigations verified that the resulting FSNs showed the traditional solvent-independent and photostable optical properties. The results implied that the novel FSNs are suitable as biomarkers in living cells and function as fluorescent visualizing agents for intracellular imaging and drug carriers.