bluuebunny/crossref_metadata_2025 huggingface dataset:
Associations between Weight Status and Situational Motivation toward Fitness Testing in Physical Education: The Mediator Role of Physical Fitness
Authors: Grao-Cruces Alberto , Racero-García Alejandro , Sánchez-Oliva David , Blanco-Luengo David , Nuviala Alberto , García-Calvo Tomás
Published: July 2020

Background: This article examines the differences in situational motivation toward fitness testing in physical education classes between non-overweight and overweight students, as well as the mediator effect of objective and perceived physical fitness on the relationship between weight status and motivation toward fitness testing. Methods: A total of 534 adolescents (298 boys, 55.80%) participated in the study. Perceived physical fitness and situational motivation toward fitness testing were measured through questionnaires, whereas weight status and physical fitness were objectively measured. Results: Overweight students had lower intrinsic motivation (p < 0.001), and higher external regulation (p < 0.01) and amotivation (p < 0.05) during fitness testing in a physical education class than their non-overweight peers. The influence of being overweight on motivation regulations toward fitness testing was mediated by objective physical fitness level for intrinsic motivation (B = −0.140), external regulation (B = 0.104) and amotivation (B = 0.146). Perceived physical fitness was also used as a second mediator between weight status and intrinsic motivation (B = −0.117). Conclusions: Strategies to improve objective and perceived physical fitness in overweight students are necessary to increase self-determined motivation during fitness testing in physical education lesson.
Addressing and Overcoming Barriers to E-Cigarette Use for Smoking Cessation in Pregnancy: A Qualitative Study
Authors: Bowker Katharine , Ussher Michael , Cooper Sue , Orton Sophie , Coleman Tim , Campbell Katarzyna Anna
Published: July 2020

E-cigarettes may have a role in supporting pregnant women who would otherwise smoke to stop smoking. The study aimed to understand pregnant women’s vaping experiences, in particular how vaping to stop smoking is facilitated and how barriers to this are overcome. We conducted semi structured telephone interviews (n = 15) with pregnant or postpartum women who vaped during pregnancy, either exclusively (n = 10) or dual-used (n = 5) (smoked and vaped). Thematic analysis was used to analyse the interviews. Two themes emerged. First, ‘facilitating beliefs’: inherent beliefs that helped women overcome barriers to vaping. These included understanding the relative safety of vaping and economic gains compared with smoking and pregnancy being a motivator to stop smoking. Second, ‘becoming a confident vaper’: accumulating sufficient skill and confidence to comfortably vape. This included experimentation with e-cigarettes to ensure nicotine dependence and sensory needs were met. Seeking social support and employing strategies to address social stigma were also important. Positive beliefs about vaping and becoming proficient at vaping were viewed as ways to overcome barriers to vaping. The theoretical domain framework informed intervention recommendations to assist pregnant smokers who have tried but cannot stop smoking to switch to vaping.
Talent Management, Affective Organizational Commitment and Service Performance in Local Government
Authors: Luna-Arocas Roberto , Lara Francisco J.
Published: July 2020

Talent management (TM) is a fundamental issue for both private and public sector companies. This study analyzes the impact of TM on service performance (SP) and the mediating role of affective organizational commitment (AOC). We analyze a sample of 104 local government employees with three measures of TM, AOC and SP. The mediation hypothesis of AOC was also raised in the study using Baron and Kenny’s methodology and Hayes PROCESS. The results reveal how AOC is a total mediating variable in the causal relationship TM → SP. This study is cross-sectional. Common-method bias is controlled in the study. The results involves a concern for improving services through the professionals who provide them, which in turn entails managing people in a way that is different, more flexible, less bureaucratic, and more client- or citizen-oriented. Given the scant research exploring the role of talent management in public services, this article offers valuable insights for scientific literature and practitioners in the public administration.
Fine-Grained Spatiotemporal Analysis of the Impact of Restricting Factories, Motor Vehicles, and Fireworks on Air Pollution
Authors: Yang Mei , Fan Hong , Zhao Kang
Published: July 2020

Aiming at improving the air quality and protecting public health, policies such as restricting factories, motor vehicles, and fireworks have been widely implemented. However, fine-grained spatiotemporal analysis of these policies’ effectiveness is lacking. This paper collected the hourly meteorological and PM2.5 data for three typical emission scenarios in Hubei, Beijing–Tianjin–Hebei (BTH), and Yangtze River Delta (YRD). Then, this study simulated the PM2.5 concentration under the same meteorological conditions and different emission scenarios based on a reliable hourly spatiotemporal random forest model ( R 2 exceeded 0.84). Finally, we investigated the fine-grained spatiotemporal impact of restricting factories, vehicles, and fireworks on PM2.5 concentrations from the perspective of hours, days, regions, and land uses, excluding meteorological interference. On average, restricting factories and vehicles reduced the PM2.5 concentration at 02:00, 08:00, 14:00, and 20:00 by 18.57, 16.22, 25.00, and 19.07 μ g / m 3 , respectively. Spatially, it had the highest and quickest impact on Hubei, with a 27.05 μ g / m 3 decrease of PM2.5 concentration and 17 day lag to begin to show significant decline. This was followed by YRD, which experienced a 23.52 μ g / m 3 decrease on average and a 23 day lag. BTH was the least susceptible; the PM2.5 concentration decreased by only 8.2 μ g / m 3 . In addition, influenced by intensive human activities, the cultivated, urban, and rural lands experienced a larger decrease in PM2.5 concentration. These empirical results revealed that restricting factories, vehicles, and fireworks is effective in alleviating air pollution and the effect showed significant spatiotemporal heterogeneity. The policymakers should further investigate influential factors of hourly PM2.5 concentrations, combining with local geographical and social environment, and implement more effective and targeted policies to improve local air quality, especially for BTH and the air quality at morning and night.
Prevalence of Insulin Resistance in the Hungarian General and Roma Populations as Defined by Using Data Generated in a Complex Health (Interview and Examination) Survey
Authors: Ádány Róza , Pikó Péter , Fiatal Szilvia , Kósa Zsigmond , Sándor János , Bíró Éva , Kósa Karolina , Paragh György , Bácsné Bába Éva , Veres-Balajti Ilona , Bíró Klára , Varga Orsolya , Balázs Margit
Published: July 2020

Data mainly from one-off surveys clearly show that the health of Roma, the largest ethnic minority of Europe, is much worse than that of the general population. However, results from comprehensive exploratory studies are missing. The aim of our study was to create a complex database for comparative and association studies to better understand the background of the very unfavourable health of Roma, especially the high burden of cardiometabolic diseases. A three-pillar (questionnaire-based, physical and laboratory examinations) health survey was carried out on randomly selected samples of the Hungarian general (HG, n = 417) and Roma (HR, n = 415) populations, and a database consisting of more than half a million datapoints was created. Using selected data, the prevalence rates of metabolic syndrome (MetS) and of its components were determined, and to estimate the risk of insulin resistance (IR), surrogate measures (the homeostasis model assessment of insulin resistance index, quantitative insulin sensitivity check index, McAuley and TyG indices and the TG/HDL-C ratio) were calculated. Receiver operating characteristic curve analysis and Youden’s method were used to define the optimal cut-off values of each IR index. The prevalence of MetS was very high in both study populations (HG: 39.8%, HR: 44.0%) with no statistically significant difference between the two groups in females or males. The prevalence of MetS showed a very marked increase in the HR 35–49 years age group. Among surrogate measures, the TyG index showed the greatest power for predicting IR/MetS at a cut-off value of 4.69 (77% sensitivity, 84% specificity) and indicated a 42.3% (HG) and 40.5% (HR) prevalence of IR. The prevalence of MetS and IR is almost equally very unfavourable in both groups; thus, the factors underlying the high premature mortality burden of Roma should be further clarified by investigating the full spectrum of risk factors available in the database, with a special focus on the access of Roma people to preventive and curative health services.
Courage in Competition: Adaptation of the Sports Courage Scale for American English and Validation of the Factor Structure with Student-Athletes at Clemson University
Authors: Konter Erkut , Kueh Yee Cheng , Kuan Garry
Published: July 2020

While courage is widely attributed to athletic pursuits, it has received little scientific attention from both researchers and applied practitioners. A reliable measurement is required to examine courage in sports and competitive activities. Therefore, this research aimed to adapt the original Turkish Sports Courage Scale-31 into American English (SCS-AE). The SCS-31 measure was translated from Turkish into the American English language by the Brislin forward and backward translation technique and language validity. Then, the translated SCS-AE was administered to 548 American university college students (Mean age = 19.02, SD = 1.21). All participants played a sport (e.g., football, soccer, basketball, gymnastics). Based on confirmatory factor analyses (CFA), 31 items of SCS-AE were reduced to 24 items with four factors (i.e., assertiveness, determination, mastery, and venturesome). The fit indices were satisfactory (RMSEA = 0.06, CFI = 0.97, SRMR = 0.06, NFI = 0.96 and NNFI = 0.97). The internal consistency measured by Cronbach alpha, ranging from 0.73 to 0.78, were considered acceptable. The convergent validity and discriminant validity of SCS-AE were also achieved. Our findings indicate strong support for research using the four-factor model of the SCS-AE and adequate support for the five-factor model with sufficient caution regarding the internal consistency of the self-sacrifice factor. While cultural differences in courage perception might exist between these countries, the findings showed more similarities than differences in courage. Results indicated that the SCS-AE is usable for research purposes in the suggested format. Future directions are discussed using the SCS-31 and SCS-AE for research.
Affective Temperament Traits and Age-Predicted Recreational Cannabis Use in Medical Students: A Cross-Sectional Study
Authors: Infortuna Carmenrita , Silvestro Steven , Crenshaw Keith , Muscatello Maria Rosaria Anna , Bruno Antonio , Zoccali Rocco Antonio , Chusid Eileen , Intrator Jordan , Han Zhiyong , Battaglia Fortunato
Published: July 2020

The use of cannabis among college students is increasing. Cannabis abuse has been proposed to be associated with personality dimensions. However, there are currently no known studies on the relationship of temperament traits and recreational cannabis use among college students. This is a cross-sectional study that investigated 328 students at a Podiatric Medical College. We evaluated the association between temperament and recreational cannabis use by the students. Temperament was investigated using the Memphis, Pisa, Paris and San Diego Auto- Questionnaire (TEMPS-A (short version)). Additionally, we assessed demographics variables and perceived stress in the context of cannabis use, and analyzed the findings using logistic regression. The prevalence of recreational cannabis use was 8.45%. Recreational cannabis use among these students was highly associated with irritable and cyclothymic temperament traits. There was no association between recreational cannabis use and perceived stress, and demographic variables or other substance use. Furthermore, logistic regression analysis indicated that higher scores in cyclothymic or irritable temperament traits are significant predictors for recreational cannabis use. Our study has identified key temperament traits, with a strong association with recreational use of cannabis of the studied student population. Our findings are useful in designing screening and educational strategies directed towards increasing the wellbeing of medical students.
Involving Parents to Help Improve Children’s Energy Balance-Related Behaviours Through a School-Based Intervention
Authors: Verhees Anke H. , Verjans-Janssen Sacha R.B. , Van Kann Dave H.H. , Kremers Stef P.J. , Vos Steven B. , Gerards Sanne M.P.L.
Published: July 2020

The Challenge Me intervention aimed to indirectly involve parents in a school-based intervention, by challenging primary school children to perform physical activity (PA) and nutrition-related activities with their parents. The aim of this study is to gain insight in whether this was a feasible strategy to engage children and parents, especially those of vulnerable populations. An exploratory cross-sectional study design was applied. Four primary schools implemented the intervention. Data consisted of challenges completed (intervention posters) and child and family characteristics (questionnaires and anthropometric measurements). Associations between challenges performed and child and family characteristics were assessed using linear regression analysis. Of the 226 study participants, 100% performed at least one challenge, and 93% performed at least one challenge involving parents. Children who performed more PA challenges were often younger, a sports club member, lived in higher socioeconomic status neighbourhoods, of Western ethnicity and from larger families. Regarding nutrition challenges involving parents, younger children performed more challenges. There was no difference in intervention engagement regarding gender, weight status, PA preference, healthy nutrition preference, or the Family PA and Family Nutrition Climate. Challenge Me has potential in involving parents in a school-based intervention. However, certain characteristics were associated with higher involvement.
Risk Assessment and Source Apportionment of Soil Heavy Metals under Different Land Use in a Typical Estuary Alluvial Island
Authors: Sun Ting , Huang Jingling , Wu Yuying , Yuan Yuan , Xie Yujing , Fan Zhengqiu , Zheng Zhijian
Published: July 2020

Understanding the environmental risks of soil heavy metals (HMs) and identifying their main sources are the essential prerequisites for the prevention and management of soil pollution. Based on a detailed survey of soil HMs (Cu, Cr, Ni, Zn, Pb, Cd, As and Hg) from different land use types (including agricultural land, construction land, wetland, and forest land) in an estuary alluvial island, the environmental risk and source apportionment of soil HMs were investigated. Altogether, 117 soil samples were taken in the study area to appraise the soil HMs environmental risk by using the geo-accumulation index (Igeo), potential ecological risk index (RI), and human health risk assessment (HRA) and to identify its main sources by using positive matrix factorization (PMF) model. The average concentrations of soil HMs (except As) surpassed their reference background values in China. There were no significant differenced in the mean concentrations of HMs in different land use types, except that the Hg concentration in the construction land was significantly higher than that in others. The results of Igeo showed that Cd pollution was unpolluted to moderately polluted, and that the others were unpolluted. The potential ecological risk level for Cd and Hg was “moderated potential risk”, while for Cu, Cr, Ni, Zn, Pb and As was “low potential risk”. Higher contamination was distributed at the west-central area. The results of the HRA indicated that the non-carcinogenic risk and the carcinogenic risk that human beings suffered from HMs in different land uses were insignificant. To more accurately identify the sources of soil HMs, the PMF model coupled with the GIS-spatial analysis was applied. The results showed that agricultural activities, natural source, industrial discharge and river transportation, and atmosphere deposition were the main determining factors for the accumulation of soil HMs in the study area, with the contribution rate of 24.25%, 23.79%, 23.84%, and 28.12%, respectively. The study provides an underlying insight needed to control of the soil HM pollutions for an estuary alluvial island.
Transformational Teaching in Physical Education and Students’ Leisure-Time Physical Activity: The Mediating Role of Learning Climate, Passion and Self-Determined Motivation
Authors: Castillo Isabel , Molina-García Javier , Estevan Isaac , Queralt Ana , Álvarez Octavio
Published: July 2020

In the context of education, this study examined the relationship between perceiving a transformational physical education (PE) teacher and student’s leisure-time physical activity (PA). Furthermore, we tested the potential mediation role of motivational learning climate, passion and self-determined motivation in this relationship. The sample was composed of 2210 high-school PE students (1145 males, 1065 females) between 16 and 20 years of age. Results of structural equation modeling revealed that the perceived transformational PE teacher–PA outcomes relationship was stronger when students perceived a task-involving climate, when they were harmoniously passionate, and when they were self-determined. We conclude that students’ health-enhancing behaviours could be improved if their PE teachers use transformational teaching style and created a task-oriented learning climate.
Effect of Ambient Air Pollution on Hospital Readmissions among the Pediatric Asthma Patient Population in South Texas: A Case-Crossover Study
Authors: Baek Juha , Kash Bita A. , Xu Xiaohui , Benden Mark , Roberts Jon , Carrillo Genny
Published: July 2020

Few studies have evaluated the association between ambient air pollution and hospital readmissions among children with asthma, especially in low-income communities. This study examined the short-term effects of ambient air pollutants on hospital readmissions for pediatric asthma in South Texas. A time-stratified case-crossover study was conducted using the hospitalization data from a children’s hospital and the air pollution data, including particulate matter 2.5 (PM2.5) and ozone concentrations, from the Centers for Disease Control and Prevention between 2010 and 2014. A conditional logistic regression analysis was performed to investigate the association between ambient air pollution and hospital readmissions, controlling for outdoor temperature. We identified 111 pediatric asthma patients readmitted to the hospital between 2010 and 2014. The single-pollutant models showed that PM2.5 concentration had a significant positive effect on risk for hospital readmissions (OR = 1.082, 95% CI = 1.008–1.162, p = 0.030). In the two-pollutant models, the increased risk of pediatric readmissions for asthma was significantly associated with both elevated ozone (OR = 1.023, 95% CI = 1.001–1.045, p = 0.042) and PM2.5 concentrations (OR = 1.080, 95% CI = 1.005–1.161, p = 0.036). The effects of ambient air pollutants on hospital readmissions varied by age and season. Our findings suggest that short-term (4 days) exposure to air pollutants might increase the risk of preventable hospital readmissions for pediatric asthma patients.
A Qualitative Examination of Water Access and Related Coping Behaviors to Understand Its Link to Food Insecurity among Rural Households in the West Region in Cameroon
Authors: Nounkeu Carole D. , Dharod Jigna M.
Published: July 2020

Food insecurity is a significant public health issue, since it causes malnutrition and engenders millions of deaths every year. A significant association is found between water and food insecurity. However, it remains unclear what are the pathways through which water shortage impacts food insecurity. Hence, a qualitative study was conducted in rural areas in Cameroon to (1) examine water access, its management, and its daily use and (2) investigate common behavior changes and coping strategies adults used in managing limited water availability in their households. Three rounds of focus group discussions and six key informant interviews were conducted with men and women. The results demonstrated that water access was limited, involving long walking distances and making several trips to the water sources. The household size, number of adults vs. children, and presence of storage containers affected water availability and its daily use. To manage limited water, coping behaviors included skipping drinking, changing cooking plans, and recycling water. In conclusion, limited water access increases food insecurity through several pathways. Governments, policy makers, and international organizations should recognize the interwoven link between water and food security. Joint actions and collaborative efforts are needed to improve success and reduce tradeoffs in achieving Sustainable Development Goals # 2 and # 6.
Bioimpedance Vector Patterns Changes in Response to Swimming Training: An Ecological Approach
Authors: Reis Joana F. , Matias Catarina N. , Campa Francesco , Morgado José P. , Franco Paulo , Quaresma Pedro , Almeida Nuno , Curto Dalia , Toselli Stefania , Monteiro Cristina P.
Published: July 2020

Background and aim: Monitoring bioelectric phase angle (PhA) provides important information on the health and the condition of the athlete. Together with the vector length, PhA constitutes the bioimpedance vector analysis (BIVA) patterns, and their joint interpretation exceeds the limits of the evaluation of the PhA alone. The present investigation aimed to monitor changes in the BIVA patterns during a training macrocycle in swimmers, trying to ascertain if these parameters are sensitive to training load changes across a 13-week training period. Methods: Twelve national and international level swimmers (four females; eight males; 20.9 ± 1.9 years; with a competitive swimming background of 11.3 ± 1.8 years; undertaking 16–20 h of pool training and 4–5 h of dry-land training per week and 822.0 ± 59.0 International Swimming Federation (FINA) points) were evaluated for resistance (R) and reactance (Xc) using a single frequency phase sensitive bioimpedance device at the beginning of the macrocycle (M1), just before the beginning of the taper period (M2), and just before the main competition of the macrocycle (M3). At the three-time assessment points, swimmers also performed a 50 m all-out first stroke sprint with track start (T50 m) while time was recorded. Results: The results of the Hotelling T2 test showed a significant vector displacement due to simultaneous R and Xc changes (p < 0.001), where shifting from top to bottom along the major axis of the R-Xc graph from M1 to M2 was observed. From M2 to M3, a vector displacement up and left along the minor axis of the tolerance ellipses resulted in an increase in PhA (p < 0.01). The results suggest a gain in fluid with a decrease in cellular density from M1 to M2 due to decrements in R and Xc. Nevertheless, the reduced training load characterizing taper seemed to allow for an increase in PhA and, most importantly, an increase of Xc, thus demonstrating improved cellular health and physical condition, which was concomitant with a significant increase in the T50 m performance (p < 0.01). Conclusions: PhA, obtained by bioelectrical R and Xc, can be useful in monitoring the condition of swimmers preparing for competition. Monitoring BIVA patterns allows for an ecological approach to the swimmers’ health and condition assessment without resorting to equations to predict the related body composition variables.
External and Total Hip Rotation Ranges of Motion Predispose to Low Back Pain in Elite Spanish Inline Hockey Players
Authors: Cejudo Antonio , Moreno-Alcaraz Víctor Jesús , Izzo Ricardo , Santonja-Medina Fernando , Sainz de Baranda Pilar
Published: July 2020

Low back pain (LBP) is a common ailment in competitive athletes. Although the association between limited range of motion (ROM) and prevalence of LBP has been widely investigated in other sports, there is no research about this topic in inline hockey (IH). The main purposes of this study in IH players were (1) to analyze the association between hip ROM and LBP and (2) to establish a diagnostic cutoff for ROM of high risk of LBP. Twenty elite IH players from the two Spanish National Teams (male and female) were assessed for passive maximum hip ROMs; the movement evaluated were the hip external [HER] and internal [HIR] rotation, hip flexion with flexed [HF-KF] and extended [HF-KE] knee, hip adduction with flexed hip [HAD-HF], hip abduction with neutral [HAB] and flexed [HAB-HF] hip, and hip extension [HE]. LBP was retrospectively monitored for the last 12 months before the date of ROM assessment by using a questionnaire. The data were analyzed via a binary logistic regression and receiver operating characteristic curves. The 70% of players had developed LBP during the retrospective study period. Significant differences between LBP group and asymptomatic group for HER (p = 0.013, d [Cohen’s effect size] = 1.17) and hip total rotation [HTR] (p = 0.032, d [Cohen’s effect size] = 1.05) were observed. The cutoff points with the greatest discriminatory capacity were 56.5° for HER and 93° for HTR ROMs.
Efficacy of a Six-Week Dispersed Wingate-Cycle Training Protocol on Peak Aerobic Power, Leg Strength, Insulin Sensitivity, Blood Lipids and Quality of Life in Healthy Adults
Authors: Wun Chun Hou , Zhang Mandy Jiajia , Ho Boon Hor , McGeough Kenneth , Tan Frankie , Aziz Abdul Rashid
Published: July 2020

Background: The aim of this study was to evaluate the efficacy of a six-week dispersed Wingate Anaerobic test (WAnT) cycle exercise training protocol on peak aerobic power (VO2peak), isokinetic leg strength, insulin sensitivity, lipid profile and quality of life, in healthy adults. Methods: We conducted a match-controlled cohort trial and participants were assigned to either the training (intervention, INT, N = 16) or non-training (control, CON, N = 17) group. INT performed 30-s WAnT bouts three times a day in the morning, afternoon and evening with each bout separated by ~4 h of rest, performed for 3 days a week for 6 weeks. Criterion measures of peak oxygen uptake (VO2peak), leg strength, insulin markers such as homeostatic model assessment (HOMA) and quantitative insulin-sensitivity check index (QUICKI), blood lipids profile and health-related quality of life (HRQL) survey were assessed before and after 6 weeks in both groups. Results: Absolute VO2peak increased by 8.3 ± 7.0% (p < 0.001) after INT vs. 0.9 ± 6.1% in CON (p = 0.41) group. Maximal voluntary contraction at 30°·s−1 of the dominant lower-limb flexors in INT increased significantly post-training (p = 0.03). There were no changes in the INT individuals’ other cardiorespiratory markers, HOMA, QUICKI, blood lipids, and HRQL measures (all p > 0.05) between pre- and post-training; but importantly, no differences were observed between INT and CON groups (all p > 0.05). Conclusions: The results indicate that 6 weeks of dispersed sprint cycle training increased cardiorespiratory fitness and dynamic leg strength but had minimal impact on insulin sensitivity, blood lipids and quality of life in the exercising individuals.
Communication as a Strategy to Promote Sports and Health Activities Designed for Adolescents
Authors: Fernández-Martínez Antonio , Pérez-Ordás Raquel , Nuviala Román , Aznar Mónica , Porcel-Gálvez Ana María , Nuviala Alberto
Published: July 2020

Physical activity reduces the risk of developing noncommunicable diseases and improves quality of life, providing health benefits for present and future generations. This is especially relevant for adolescents. Educational institutions are promoters of healthy habits through the organisation of different activities such as extracurricular sports programmes. These activities increase the rates of sports practice among adolescents. The literature shows that the perceived quality of sports and health services is an antecedent of users’ behavioural intentions. The aim of this paper is to find out whether communication from educational/sports organisations influences adolescents’ intentions to continue engaging in physical activity. A total of 1080 students participated, with a mean age of 13.76 ± 1.39 years, 34.1% of whom were girls. Tests were conducted to verify the validity and reliability of the model that relates communication with value, satisfaction, and future intentions. Tests were conducted to verify the validity (average variance extracted was between 0.754 and 0.583) and reliability (composite reliability was between 0.925 and 0.813) of the model that relates communication with value, satisfaction, and future intentions. Confirmatory analyses and factor invariance tests were performed. The results revealed that communication is an antecedent of value, satisfaction, and future intentions. In conclusion, communication is a good strategy to consolidate sporting habits in both male and female adolescents.
Correlates of Sleep Disturbance among Older Adults with Mild Cognitive Impairment: A Cross-Sectional Study
Authors: Song Dan , Yu Doris S. F. , Sun Qiuhua , He Guijuan
Published: July 2020

Individuals with mild cognitive impairment (MCI) are at high risk for dementia development. Sleep disturbance is often overlooked in MCI, although it is an important risk factor of cognitive decline. In the absence of a cure for dementia, managing the risk factors of cognitive decline in MCI is likely to delay disease progression. To develop interventions for sleep disturbance in MCI, its related factors should be explored. This study aimed to identify and compare the correlates of sleep disturbance in older adults with MCI and those in cognitively healthy older adults. A comparative cross-sectional study was adopted. Data were obtained from 219 Chinese community-dwelling older adults (female: 70.3%), which consisted of 127 older adults with MCI and 92 age-matched cognitively healthy controls. The candidate correlates of sleep disturbance included socio-demographic correlates, health-related factors, lifestyle-related factors and psychological factor. Descriptive, correlational and regression statistics were used for data analysis. The prevalence of sleep disturbance in MCI was 70.1% compared to that of 56.5% in cognitively healthy controls (p < 0.001). The multivariate analysis indicated that, in participants with MCI, depressive symptoms (Beta = 0.297, p = 0.001), comorbidity burden (Beta = 0.215, p = 0.012) and physical activity (Beta = −0.297, p = 0.001) were associated with sleep disturbance. However, in the cognitively healthy controls, only depressive symptoms (Beta = 0.264, p = 0.028) and comorbidity burden (Beta = 0.361, p = 0.002) were associated with sleep disturbance. This finding highlights that sleep disturbance is sufficiently prominent to warrant evaluation and management in older adults with MCI. Furthermore, the findings elucidate several important areas to target in interventions aimed at promoting sleep in individuals with MCI.
Factors Influencing the Adoption of Shared Autonomous Vehicles
Authors: Yuen Kum Fai , Huyen Do Thi Khanh , Wang Xueqin , Qi Guanqiu
Published: July 2020

Shared autonomous vehicles (SAVs), which have several potential benefits, are an emerging innovative technology in the market. However, the successful operation of SAVs largely depends on the extent of travellers’ intention to adopt them. This study aims to analyse the factors that influence the adoption of SAVs by integrating two theoretical perspectives: the unified theory of acceptance and use of technology 2 (UTAUT2) and the theory of planned behaviour (TPB). A valid survey sample of 268 participants in Da Nang, Vietnam was collected. Subsequently, structural equation modelling was deployed to test the research model. The results indicate that the five dimensions of UTUAT2: performance expectation, effort expectation, habit, price value and hedonic motivation, are mediated by the attitudes toward using SAVs. Further, the TPB constructs, namely attitude, subject norm, perceived behavioural control, along with its perceived facilitating conditions, are all effective predictors of intention to use SAVs. The findings of this study can serve as a crucial resource for transport operators and the government to enhance transportation services and policies.
The Outcome and Implications of Public Precautionary Measures in Taiwan–Declining Respiratory Disease Cases in the COVID-19 Pandemic
Authors: Hsieh Chih-Chia , Lin Chih-Hao , Wang William Yu Chung , Pauleen David J. , Chen Jengchung Victor
Published: July 2020

With the rapid development of the COVID-19 pandemic, countries are trying to cope with increasing medical demands, and, at the same time, to reduce the increase of infected numbers by implementing a number of public health measures, namely non-pharmaceutical interventions (NPIs). These public health measures can include social distancing, frequent handwashing, and personal protective equipment (PPE) at the personal level; at the community and the government level, these measures can range from canceling activities, avoiding mass gatherings, closing facilities, and, at the extreme, enacting national or provincial lockdowns. Rather than completely stopping the infectious disease, the major purpose of these NPIs in facing an emerging infectious disease is to reduce the contact rate within the population, and reduce the spread of the virus until the time a vaccine or reliable medications become available. The idea is to avoid a surge of patients with severe symptoms beyond the capacity of the hospitals’ medical resources, which would lead to more mortality and morbidity. While many countries have experienced steep curves in new cases, some, including Hong Kong, Vietnam, South Korea, New Zealand, and Taiwan, seem to have controlled or even eliminated the infection locally. From its first case of COVID-19 on the 21 January until the 12 May, Taiwan had 440 cases, including just 55 local infections, and seven deaths in total, representing 1.85 cases per 100,000 population and a 1.5% death rate (based on the Worldometer 2020 statistics of Taiwan’s population of 23.8 million). This paper presents evidence that spread prevention involving mass masking and universal hygiene at the early stage of the COVID-19 pandemic resulted in a 50% decline of infectious respiratory diseases, based on historical data during the influenza season in Taiwan. These outcomes provide potential support for the effectiveness of widely implementing public health precaution measures in controlling COVID-19 without a lockdown policy.
Sodium Intake and Chronic Kidney Disease
Authors: Borrelli Silvio , Provenzano Michele , Gagliardi Ida , Michael Ashour , Liberti Maria , De Nicola Luca , Conte Giuseppe , Garofalo Carlo , Andreucci Michele
Published: July 2020

In Chronic Kidney Disease (CKD) patients, elevated blood pressure (BP) is a frequent finding and is traditionally considered a direct consequence of their sodium sensitivity. Indeed, sodium and fluid retention, causing hypervolemia, leads to the development of hypertension in CKD. On the other hand, in non-dialysis CKD patients, salt restriction reduces BP levels and enhances anti-proteinuric effect of renin–angiotensin–aldosterone system inhibitors in non-dialysis CKD patients. However, studies on the long-term effect of low salt diet (LSD) on cardio-renal prognosis showed controversial findings. The negative results might be the consequence of measurement bias (spot urine and/or single measurement), reverse epidemiology, as well as poor adherence to diet. In end-stage kidney disease (ESKD), dialysis remains the only effective means to remove dietary sodium intake. The mismatch between intake and removal of sodium leads to fluid overload, hypertension and left ventricular hypertrophy, therefore worsening the prognosis of ESKD patients. This imposes the implementation of a LSD in these patients, irrespective of the lack of trials proving the efficacy of this measure in these patients. LSD is, therefore, a rational and basic tool to correct fluid overload and hypertension in all CKD stages. The implementation of LSD should be personalized, similarly to diuretic treatment, keeping into account the volume status and true burden of hypertension evaluated by ambulatory BP monitoring.
Reversible Growth-Arrest of a Spontaneously-Derived Human MSC-Like Cell Line
Authors: Melzer Catharina , Jacobs Roland , Dittmar Thomas , Pich Andreas , von der Ohe Juliane , Yang Yuanyuan , Hass Ralf
Published: July 2020

Life cycle limitation hampers the production of high amounts of primary human mesenchymal stroma-/stem-like cells (MSC) and limits cell source reproducibility for clinical applications. The characterization of permanently growing MSC544 revealed some differentiation capacity and the simultaneous presence of known MSC markers CD73, CD90, and CD105 even after continuous long-term culture for more than one year and 32 passages. The expression of CD13, CD29, CD44, and CD166 were identified as further surface proteins, all of which were also simultaneously detectable in various other types of primary MSC populations derived from the umbilical cord, bone marrow, and placenta suggesting MSC-like properties in the cell line. Proliferating steady state MSC544 exhibited immune-modulatory activity similar to a subpopulation of long-term growth-inhibited MSC544 after 189d of continuous culture in confluency. This confluent connective cell layer with fibroblast-like morphology can spontaneously contract and the generated space is subsequently occupied by new cells with regained proliferative capacity. Accordingly, the confluent and senescence-associated beta-galactosidase-positive MSC544 culture with about 95% G0/G1 growth-arrest resumed re-entry into the proliferative cell cycle within 3d after sub-confluent culture. The MSC544 cells remained viable during confluency and throughout this transition which was accompanied by marked changes in the release of proteins. Thus, expression of proliferation-associated genes was down-modulated in confluent MSC544 and re-expressed following sub-confluent conditions whilst telomerase (hTERT) transcripts remained detectable at similar levels in both, confluent growth-arrested and proliferating MSC544. Together with the capability of connective cell layer formation for potential therapeutic approaches, MSC544 provide a long term reproducible human cell source with constant properties.
Extracellular Matrix Stiffness and Composition Regulate the Myofibroblast Differentiation of Vaginal Fibroblasts
Authors: Ruiz-Zapata Alejandra M. , Heinz Andrea , Kerkhof Manon H. , van de Westerlo-van Rijt Cindy , Schmelzer Christian E. H. , Stoop Reinout , Kluivers Kirsten B. , Oosterwijk Egbert
Published: July 2020

Fibroblast to myofibroblast differentiation is a key feature of wound-healing in soft tissues, including the vagina. Vaginal fibroblasts maintain the integrity of the vaginal wall tissues, essential to keep pelvic organs in place and avoid pelvic organ prolapse (POP). The micro-environment of vaginal tissues in POP patients is stiffer and has different extracellular matrix (ECM) composition than healthy vaginal tissues. In this study, we employed a series of matrices with known stiffnesses, as well as vaginal ECMs, in combination with vaginal fibroblasts from POP and healthy tissues to investigate how matrix stiffness and composition regulate myofibroblast differentiation in vaginal fibroblasts. Stiffness was positively correlated to production of α-smooth muscle actin (α-SMA). Vaginal ECMs induced myofibroblast differentiation as both α-SMA and collagen gene expressions were increased. This differentiation was more pronounced in cells seeded on POP-ECMs that were stiffer than those derived from healthy tissues and had higher collagen and elastin protein content. We showed that stiffness and ECM content regulate vaginal myofibroblast differentiation. We provide preliminary evidence that vaginal fibroblasts might recognize POP-ECMs as scar tissues that need to be remodeled. This is fundamentally important for tissue repair, and provides a rational basis for POP disease modelling and therapeutic innovations in vaginal reconstruction.
Absolute Binding Free Energy Calculations for Highly Flexible Protein MDM2 and Its Inhibitors
Authors: Singh Nidhi , Li Wenjin
Published: July 2020

Reliable prediction of binding affinities for ligand-receptor complex has been the primary goal of a structure-based drug design process. In this respect, alchemical methods are evolving as a popular choice to predict the binding affinities for biomolecular complexes. However, the highly flexible protein-ligand systems pose a challenge to the accuracy of binding free energy calculations mostly due to insufficient sampling. Herein, integrated computational protocol combining free energy perturbation based absolute binding free energy calculation with free energy landscape method was proposed for improved prediction of binding free energy for flexible protein-ligand complexes. The proposed method is applied to the dataset of various classes of p53-MDM2 (murine double minute 2) inhibitors. The absolute binding free energy calculations for MDMX (murine double minute X) resulted in a mean absolute error value of 0.816 kcal/mol while it is 3.08 kcal/mol for MDM2, a highly flexible protein compared to MDMX. With the integration of the free energy landscape method, the mean absolute error for MDM2 is improved to 1.95 kcal/mol.
Caffeine: An Overview of Its Beneficial Effects in Experimental Models and Clinical Trials of Parkinson’s Disease
Authors: Schepici Giovanni , Silvestro Serena , Bramanti Placido , Mazzon Emanuela
Published: July 2020

Parkinson’s Disease (PD) is a neurological disease characterized by the progressive degeneration of the nigrostriatal dopaminergic pathway with consequent loss of neurons in the substantia nigra pars compacta and dopamine depletion. The cytoplasmic inclusions of α-synuclein (α-Syn), known as Lewy bodies, are the cytologic hallmark of PD. The presence of α-Syn aggregates causes mitochondrial degeneration, responsible for the increase in oxidative stress and consequent neurodegeneration. PD is a progressive disease that shows a complicated pathogenesis. The current therapies are used to alleviate the symptoms of the disease without changing its clinical course. Recently, phytocompounds with neuroprotective effects and antioxidant properties such as caffeine have aroused the interest of researchers. The purpose of this review is to summarize the preclinical studies present in the literature and clinical trials recorded in ClinicalTrial.gov, aimed at illustrating the effects of caffeine used as a nutraceutical compound combined with the current PD therapies. Therefore, the preventive effects of caffeine in the neurodegeneration of dopaminergic neurons encourage the use of this alkaloid as a supplement to reduce the progress of the PD.
Aspirin Induces Mitochondrial Ca2+ Remodeling in Tumor Cells via ROS‒Depolarization‒Voltage-Gated Ca2+ Entry
Authors: Fujikawa Itsuho , Ando Takashi , Suzuki-Karasaki Manami , Suzuki-Karasaki Miki , Ochiai Toyoko , Suzuki-Karasaki Yoshihiro
Published: July 2020

Aspirin (acetylsalicylic acid) and its metabolite salicylate, have an anti-melanoma effect by evoking mitochondrial dysfunction through poorly understood mechanisms. Depolarization of the plasma membrane potential leads to voltage-gated Ca2+ entry (VGCE) and caspase-3 activation. In the present study, we investigated the role of depolarization and VGCE in aspirin’s anti-melanoma effect. Aspirin and to a lesser extent, salicylate (≥2.5 mM) induced a rapid (within seconds) depolarization, while they caused comparable levels of depolarization with a lag of 2~4 h. Reactive oxygen species (ROS) generation also occurred in the two-time points, and antioxidants abolished the early ROS generation and depolarization. At the same concentrations, the two drugs induced apoptotic and necrotic cell death in a caspase-independent manner, and antioxidants and Ca2+ channel blockers prevented cell death. Besides ROS generation, reduced mitochondrial Ca2+ (Ca2+m) and mitochondrial membrane potential preceded cell death. Moreover, the cells expressed the Cav1.2 isoform of l-type Ca2+ channel, and knockdown of Cav1.2 abolished the decrease in Ca2+m. Our findings suggest that aspirin and salicylate induce Ca2+m remodeling, mitochondrial dysfunction, and cell death via ROS-dependent depolarization and VGCE activation.
Investigating REPAIRv2 as a Tool to Edit CFTR mRNA with Premature Stop Codons
Authors: Melfi Raffaella , Cancemi Patrizia , Chiavetta Roberta , Barra Viviana , Lentini Laura , Di Leonardo Aldo
Published: July 2020

Cystic fibrosis (CF) is caused by mutations in the gene encoding the transmembrane conductance regulator (CFTR) protein. Some CF patients are compound heterozygous or homozygous for nonsense mutations in the CFTR gene. This implies the presence in the transcript of premature termination codons (PTCs) responsible for a truncated CFTR protein and a more severe form of the disease. Aminoglycoside and PTC124 derivatives have been used for the read-through of PTCs to restore the full-length CFTR protein. However, in a precision medicine framework, the CRISPR/dCas13b-based molecular tool “REPAIRv2” (RNA Editing for Programmable A to I Replacement, version 2) could be a good alternative to restore the full-length CFTR protein. This RNA editing approach is based on the targeting of the deaminase domain of the hADAR2 enzyme fused to the dCas13b protein to a specific adenosine to be edited to inosine in the mutant mRNA. Targeting specificity is allowed by a guide RNA (gRNA) complementarily to the target region and recognized by the dCas13b protein. Here, we used the REPAIRv2 platform to edit the UGA PTC to UGG in different cell types, namely IB3-1 cells, HeLa, and FRT cells engineered to express H2BGFPopal and CFTRW1282X, respectively.
Evaluating the Performance of a Non-Bonded Cu2+ Model Including Jahn−Teller Effect into the Binding of Tyrosinase Inhibitors
Authors: Martins Lucas Sousa , Lameira Jerônimo , Kruger Hendrik G. , Alves Cláudio Nahum , Silva José Rogério A.
Published: July 2020

Tyrosinase (TYR) is a metalloenzyme classified as a type-3 copper protein, which is involved in the synthesis of melanin through a catalytic process beginning with the conversion of the amino acid l-Tyrosine (l-Tyr) to l-3,4-dihydroxyphenylalanine (l-DOPA). It plays an important role in the mechanism of melanogenesis in various organisms including mammals, plants, and fungi. Herein, we used a combination of computational molecular modeling techniques including molecular dynamic (MD) simulations and the linear interaction energy (LIE) model to evaluate the binding free energy of a set of analogs of kojic acid (KA) in complex with TYR. For the MD simulations, we used a dummy model including the description of the Jahn–Teller effect for Cu2+ ions in the active site of this enzyme. Our results show that the LIE model predicts the TYR binding affinities of the inhibitor in close agreement to experimental results. Overall, we demonstrate that the classical model provides a suitable description of the main interactions between analogs of KA and Cu2+ ions in the active site of TYR.