bluuebunny/crossref_metadata_2025 huggingface dataset:
“Plan de Negocio para la introducción del producto Crédito por Convenio en las pequeñas y medianas empresas de Lima”
Authors:   , Antúnez de Mayolo Corzo Agustín Bernardo , Quispe Machaca Angela Estela , Pacheco Falcón De Gomez María Isabel
Published: May 2022

El presente plan tiene como objetivo: Proponer un Plan de Negocios para introducir el Producto Crédito-Convenio en las pequeñas y medianas empresas (Pymes) de Lima a través de una Cooperativa de Ahorro y Crédito, y con la finalidad de incrementar el número de socios, reforzar la marca y brindar un mejor servicio de acuerdo al mercado. En tal sentido el trabajo inicia con el análisis del entorno externo e interno de la empresa, encontrando oportunidades ligadas al crecimiento de la demanda, uso de nuevas tecnologías de información y comunicación, la actual legislación que contribuye al crecimiento de las cooperativas. Así mismo las amenazas relacionadas con el ingreso de nuevos competidores (Banca Múltiple) en el segmento micro financiero, el alto nivel de informalidad en las Pymes y la inestabilidad política del país. Con relación al mercado se encontró la oportunidad de introducir el producto (Crédito- Convenio) en 1,079 empresas (Pymes) que tienen entre 50 y 100 trabajadores, que por su tamaño no resultan atractivas para las Cooperativas que ofrecen el mismo servicio tales como: Cooperativas de Nivel 1, Nivel 2, CMAC y CRAC. Al desarrollar el trabajo de campo (Empleador y trabajador) se obtuvo como resultado tres principales variables que influyen en la decisión de compra: 1- Menor tiempo de atención de la solicitud de crédito. 2- Realización de los trámites por vía digital. 3- Abono electrónico del préstamo en la cuenta del cliente, siempre que se mantenga una tasa de promedio de mercado. Adicionalmente se tomaron 400 encuestas a trabajadores que laboran en las empresas del segmento objetivo, determinándose que un 49% acepta el concepto del Crédito – Convenio (Descuento por planilla). Dentro del 49% grupo que prefiere el descuento por planilla, un 68% acepta las ventajas propuestas: servicio rápido (24 horas), trámite digital y una tasa de mercado promedio del 21%, de tal forma que nuestro mercado objetivo es de 34%. Finalmente, con la aplicación del Plan de Negocios propuesto el flujo de caja a cinco años da como resultado un VAN de S/ 468,508 con una TIR de 42%, valores que permiten aceptar el proyecto.
Sistema móvil de gestión comercial para una empresa de calzados
Authors:   , Malex Trillo Gianmarco , Sandoval Sánchez Daniel Ernesto
Published: May 2022

La industria del calzado en el Perú es un sector relevante en la economía por su participación y la generación de puestos de trabajo. Se considera como unas de las actividades minoristas más importantes del país. El mercado de calzados en el Perú se caracteriza por ser bastante competitivo, con colaboración de variadas marcas tanto nacionales como internacionales. Por otro lado, es un producto que ha dado espacio para la diferenciación y que cambia temporada tras temporada siguiendo las últimas tendencias de moda. La empresa es una fábrica de calzados el cual comercializa calzados a través de sus asesores de venta. Estos asesores presentan diversos problemas, entre ellos los más importantes: la información con la que cuenta de sus clientes, productos, pedidos, etc., es escaza o desactualizada; la entrega de pedidos se ha realiza a destiempo o con retrasos; el crédito de los clientes no está controlado; el pago de moras o cuotas no se realiza adecuadamente; en la venta por consignación, frecuentemente se cometen errores en la generación de cuotas. Antes la situación problemática existente se plantea el desarrollo de un sistema móvil con el objetivo de mejorar la productividad de los asesores de venta en sus actividades diarias, lograr un mejor manejo de la información en la empresa e incrementar la rentabilidad por medio de la venta de calzados. El desarrollo de la solución comprende las siguientes fases: modelado del negocio, modelado del sistema, arquitectura del software, construcción, calidad, pruebas de software y la gestión del proyecto.
Centro Cultural en el Callao
Authors:   , Marchena Suarez Silvana
Published: May 2022

La Provincia Constitucional del Callao alberga el puerto más importante del Perú, el cuál ha ido convirtiendo la Zona Monumental del Callao en un polo industrial que ha dejado de lado el valor histórico y cultural de la ciudad portuaria. El Centro Cultural busca revalorizar la zona con una propuesta urbano – paisajista que conecta la ciudad con el mar por medio de un proyecto de carácter cultural- recreativo cuyo diseño toma de partida la flexibilidad espacial como estrategia principal para el desarrollo del programa. La propuesta parte de un eje central como espacio articulador, que conecta con todas las actividades programáticas duras del centro convirtiendo las circulaciones, en espacios de jerarquía que pasan de ser espacios de tránsito a espacios abiertos de permanencia que generan actividades de ocio, cultura y arte las cuales varían dependiendo del programa en el que se las inserte.
Centro Pediátrico de Rehabilitación en Los Olivos
Authors:   , Pantoja Falconi Antonella Irma De Lourdes
Published: May 2022

Este proyecto consiste en el diseño de un Centro de Salud del Primer Nivel de Atención especializado en la rehabilitación integral de niños y adolescentes. Se basa en una arquitectura terapéutica con la que se diseña un establecimiento de salud generando espacios amplios y abiertos hacia jardines terapéuticos, integrando los espacios naturales y recreativos con las áreas de tratamiento. Se busca resolver la problemática de nuestra ciudad en la cual tenemos un déficit de centros de salud especializados en la rehabilitación pediátrica. El proyecto se ubica estratégicamente en el distrito de Los Olivos por la demanda en este sector de la población, y la falta de centros de salud de la zona. La población a servir son menores de 18 años con ciertas limitaciones, para que puedan desarrollarse y reintegrarse en la sociedad de manera adecuada.
Splenic Infarction Following Thyroidectomy: An Unusual Case in the Emergency Department
Authors: VURAL Nafis
Published: May 2022

Splenic infarction is a rare diagnosis in the emergency department and can easily mimic other causes of acute abdomen. Cardioembolic and hematological disorders, inflammatory (eg pancreatitis) and infectious (eg sepsis) conditions, postoperative conditions, malignant neoplasms and atherosclerosis are some of the risk factors. Papillary thyroid carcinoma is the most common histologic type of differentiated thyroid malignancy and is known as the least aggressive thyroid carcinoma. A 46-year-old male patient was admitted to the emergency department with the complaint of abdominal pain for five day. When the patient's anamnesis was deepened, it was learned that the patient had been operated on 5 days before the papillary thyroid cancer, the abdominal pain started the day after the surgery, and the pain showed an increasing and decreasing course. Contrast-enhanced abdominal CT performed on the patient revealed perfusion defects in the posterior and lateral parts of the spleen. The diagnosis of splenic infarction should also be kept in mind in patients presenting to the emergency department with left upper quadrant pain, especially in patients who have recently undergone a surgical operation, have a previous history of thrombotic and vascular disease, and have any malignancy.
Uniportal thoracoscopic decortication: start away from empyema approach
Authors: Elkhayat Hussein , Gamal Montaser , Ahmed Yousef , Aboelfadel Abdelazim , Omer Amany
Published: May 2022

Aim: Video-assisted thoracoscopic surgery (VATS) is now a gold standard in treating early-stage empyema, while much debate still exists considering the effectiveness of this approach for late-stage empyema. Most concerns arise from the crowded rib cage, narrow working space, and ability to free trapped lungs quickly, mainly if uniportal VATS is used. However, unlike uniportal VATS lobectomy, there is no consensus about standard steps for uniportal VATS decortication to ensure smooth and effective surgery. We try to offer standard, easy-to-replicate steps for this approach to evaluate the efficacy and safety of the “start away from empyema” approach for uniportal VATS decortication in the management of stage II and III empyema. Methods: A prospective case series study has been conducted on 25 patients. All patients with stage II and III empyema were admitted to the Cardiothoracic Surgery Department between October 2017 and March 2020. VATS procedure was done under general anesthesia by a double-lumen tube for selective ventilation. Demographic data, preoperative diagnosis, intraoperative findings, hospital stay, and complications were recorded. Results: Twenty-five patients were enrolled in this study with a median age of 35 years. The duration of illness ranged between 14 and 60 days, with a median (IQR) of 25 (17) days. In (88%) of the patients, there was a successful outcome defined as a near-complete resolution on Chest X-ray (scoring of 3 or 4) and chest US (scoring of 2 or 3). Only three (12%) patients had a partially successful outcome. All patients with partial success suffered from infection associated with malignant effusion. Conclusion: “Start away from empyema” approach for uniportal VATS decortication in stage II and III empyema could be safe and effective in treating loculated nonmalignant pleural effusion.
Evaluation of Biological Activity of Natural Compounds: Current Trends and Methods
Authors: Barba-Ostria Carlos , Carrera-Pacheco Saskya E. , Gonzalez-Pastor Rebeca , Heredia-Moya Jorge , Mayorga-Ramos Arianna , Rodríguez-Pólit Cristina , Zúñiga Johana , Arias-Almeida Benjamin , Guamán Linda P.
Published: May 2022

Natural compounds have diverse structures and are present in different forms of life. Metabolites such as tannins, anthocyanins, and alkaloids, among others, serve as a defense mechanism in live organisms and are undoubtedly compounds of interest for the food, cosmetic and pharmaceutical industries. Plants, bacteria, and insects represent a source of biomolecules with diverse activities, poorly studied in many cases. To use these molecules for different applications, it is essential to know their structure, concentrations, and biological activity potential. In vitro techniques that evaluate the biological activity of the molecules of interest have been developed since the 1950s. Currently, different methodologies have emerged to overcome some of the limitations of these traditional techniques, mainly the reduction of time and costs. However, emerging technologies continue to appear due to the urgent need to expand the analysis capacity of a growing number of reported biomolecules and the lack of therapeutic options to treat various diseases. This review presents an updated summary of the conventional and current methods to evaluate natural compounds' biological activity, including a diagram that summarizes the minimum techniques essential for correctly assessing molecules with biological potential.
Power Quality Enhancement For Decentralized Microgrids in Island Mode
Authors: Rasheed Muhammad Arshad , Gelani Hasan Erteza , Tahir Hira , Abid Muhammad Irfan , Saeed Faisal
Published: May 2022

The microgrid is a small-scale, autonomous decentralized power plant with its own distributed generation, storage capacity and multiple loads, with the capacity to function in grid interconnected and an island mode. The decentralized control of microgrids with parallel operated voltage source converters (VSCs) is proposed in this paper to improve power quality using a machine learning approach. The DNN based MPPT controller is proposed and its best performance is presented. The SRF-PLL is utilized for AC side synchronization in VSC control. The proposed microgrid involves two PV arrays fed to two voltage source converters along with their independent controls, connected in parallel through LC filters and line coupling transformers and serves the loads at PCC. The proposed model is simulated using MATLAB/Simulink. The dq-framed inner loop control is employed to individually regulate the real and reactive power at the point of common coupling. Furthermore, the proposed model is analyzed and compared by employing a mathematical model of AC system dynamics, inner loop control, output voltage quality, AC harmonic spectrum analysis, and total harmonic distortion (THD) in both grid interconnected and island mode. In island mode, AC harmonic spectrum and THD are accomplished within the permissible range.
Expanding Urban Tree Species Diversity in Florida (USA): Challenges and Opportunities for Practitioners
Authors: Hilbert Deborah , Koeser Andrew , Andreu Michael , Clarke Mysha , Hansen Gail , Roman Lara , Thetford Mack
Published: May 2022

While many practitioners and experts understand the risks associated with low urban tree diversity, they often lack the ability to rectify issues they encounter on their own. The current system of tree production and procurement is complex – shaped by market pressures, nursery and site constraints, local governance, and differing professional objectives among those who grow, specify, and plant trees. To understand this complexity as well as constraints to- and opportunities for increasing urban tree diversity, we conducted a series of focus groups comprised of nursery growers, landscape architects, and urban foresters. Our results highlight a significant list of considerations and constraints to diversity (both shared among green industries and some specific to growers or purchasers). More importantly, in discussing our findings we outline actionable strategies for increasing urban tree diversity.
Jeans instability of an astrophysical self-gravitating medium in the presence of high radiation pressure and diffusion transfer of radiation
Authors: Kolesnichenko Aleksandr Vladimirovich
Published: May 2022

Within the framework of the problem of modeling the evolution of a protostellar disk, the influence of radiation on the Jeans gravitational instability for a self-gravitating optically thick (for intrinsic infrared radiation) gas-dust medium is discussed, taking into account the influence of radiation pressure and diffusion transfer of radiation on the critical wavelength of the perturbing wave. Two approximations of radiative diffusion are considered: 1. the case of ideal radiative equilibrium, when the temperatures of matter and radiation are the same; 2. the case of the time dependence of the radiation field, when there is an energy decoupling between radiation and matter. Using the analysis of the normal regime, dispersion relations are derived that allow one to obtain modifications of the Jeans gravitational instability criterion under the influence of radiation pressure and radiation diffusion. In particular, it is shown that, in contrast to local radiation equilibrium, when the acoustic velocity of the gas coincides with the isothermal speed of sound, in the case of a difference in the temperatures of radiation and gas, the perturbing wave propagates with the adiabatic speed of sound in the gas. The results obtained are aimed at solving the problem of gravitational instability of individual massive protostellar disks or self-gravitating radiation media characterized by large optical depths for their own infrared radiation transformed by dust.
Education and its praxis
Authors: Rodrigues Aurea Maria Pires , Santos Leandro dos
Published: May 2022

This work aims to problematize discursive practices that shape some educational models crossed by traditional teaching models. Based on a fragmented and positivist way of seeing and recognizing the world, they also intend to point out inventive practices. These practices stand for integrative education by understanding education as part of a socio-historical-economic context. To do so, we will also question how psychological theories were elaborated to explain learning processes. They feed back a traditional educational paradigm in line with some educational models. This research approaches the problem qualitatively. It is also exploratory, seeking to expand knowledge around a phenomenon (Zanella, 2009). We will collect data through bibliographic research, through texts that point out the discussion raised. To analyze this data, we will use the Content Analysis technique.
Adoption of Improved Maize Varieties Practices by Small Holder Farmers in Abuna Gindeberat, Ethiopia
Authors: Beyene Adunaa Amante
Published: May 2022

Background of study: Improving agricultural performance is one of the essential issues that must be addressed in order to improve the food security of the majority of farmers. The adoption of improved high yielding maize varieties is not extensively implemented among small holder farmers, despite the fact that maize is one of the most stable foods populated and its production is widely practiced by farmers in all agro-ecological conditions of the district. The purpose of this study was to examine adoption practices of improved maize varieties in order to generate data that might be used to improve adoption practices. Methodology: A three-stage sample strategy was used in this study, which comprised a purposeful selection of districts and Kebeles, as well as random sampling. Stratified sampling was used to identify adopters and non-adopters, while simple random sampling was used to choose respondents. The study is based on primary data collected from a sample of 143 respondents. Results: The study discovered that adopters and non-adopters of improved maize varieties differ in their land preparation, fertilizer application, topdressing, weeding and protection practices, as well as seed preparation and seedling assessment.Conclusion: adoption decision and practices of adoption of improved maize varieties were driven by a host of institutional, demographic, psychological and socio-economic factors.
Allelic variations in phenology genes of eastern U.S. soft winter and Korean winter wheat varieties and their associations with heading date
Authors: Ma Fengyun , Brown-Guedira Gina , Kang Moonseok , Baik Byung-Kee
Published: May 2022

Wheat heading time is genetically controlled by phenology genes including vernalization (Vrn), photoperiod (Ppd) and earliness per se (Eps) genes. Characterization of the existing genetic variation in the phenology genes of wheat would provide breeding programs with valuable information necessary for the development of wheat varieties well-adapted to the local environment and early-maturing traits suitable for double-cropping system. One hundred forty-nine eastern U.S. soft winter (ESW) and 32 Korean winter (KW) wheat varieties were characterized using molecular markers for Vrn, Ppd, Eps and reduced-height (Rht) genes, and phenotyped for heading date (HD) in eastern U.S. region. The predominant alleles in ESW and KW varieties were the Ppd-A1b, Ppd-B1b and Ppd-D1a alleles at the Ppd-1 loci and the vrn-B1-Neuse and vrn-D3b alleles at the Vrn-B1 and Vrn-D3 loci. A single copy of the vrn-A1 allele was observed in 81.3% of KW varieties, but only in one ESW variety. The genetic variations for HD of ESW varieties were largely contributed by the Ppd-B1, Ppd-D1 and Vrn-D3 loci. The Rht-D1 locus largely contributed to the genetic variation for HD of KW varieties. A phenology gene profile at four major loci (Ppd-B1b/Ppd-D1a/vrn-A1(three or more copies)/vrn-D3b) was predominantly observed in 52.8% of ESW varieties, while 76.7% of KW varieties carried one of these two phenology gene profiles: Ppd-B1b/Ppd-D1a/vrn-A1(one copy)/vrn-D3a or Ppd-B1b/Ppd-D1a/vrn-A1(one copy)/vrn-D3b. KW varieties headed on average 14 days earlier than ESW varieties in each crop year, largely due to the presence of a single copy of the vrn-A1 allele in the former.
Success rate of ultrasound-guided compression in management of femoral artery pseudoaneurysm following coronary artery angiography
Authors: Sobhiyeh Mohammadreza , Salehi Mohammad gharib , Kermanshahi Amirreza Jafari , Molavi Masoumeh
Published: May 2022

Background: Iatrogenic femoral artery pseudoaneurysm, a rare but significant complication, could be managed by ultrasound-guided compression. This study decided to evaluate the success rate of this method and the risk factors associated with failure.Methods: In this cross-sectional study performed in two university hospitals, all patients who developed pseudoaneurysm of the femoral artery after coronary angiography for six years (2013 to 2018) and underwent ultrasound-guided compression by a vascular surgeon were included. Necessary information, including gender, age, body mass index (BMI), pseudoaneurysm neck diameter, diabetes, hypertension, smoking, anemia, and coronary heart disease, were collected. Then, the success rate of the treatment was evaluated, and the risk factors associated with failure were determined using the regression analysis.Results: Forty-five patients (25 males and 20 females) with a mean age of 61.6 years developed the complication. Treatment was successful in 32 patients (71.1%) but failed in 13 patients (28.9%). Logistic regression results showed that BMI ≥ 30 kg/m2 (OR (odds ratio)= 5.7, 95% CI: 1.2 to 26.6, P= 0.02) and pseudoaneurysm neck diameter of ≥ 4 mm (OR= 6.3, 95% CI: 1.3 to 31.3, P= 0.02) significantly associated with treatment failure. Conclusion: Obesity (BMI ≥ 30) and neck diameter of ≥ 4 mm were significant risk factors for failure of ultrasound-guided compression of femoral artery pseudoaneurysm. Taking more precautions in patients who have these two risk factors may help reduce the failure rate of this technique.
Enhanced prediction of short-term prognosis of community-acquired pneumonia using random survival forests by incorporating suppression of tumorigenicity 2 as a feature
Authors: Zhang Teng , Zeng Yifeng , Lin Runpei , Xue Mingshan , Liu Mingtao , Li Yusi , Zhen Yingjie , Li Ning , Cao Wenhan , Wu Sixiao , Zhu Huiqin , Zhang Qi , Sun Baoqing
Published: May 2022

Purpose: The development of biomarkers and models can screen inpatients with a low probability of cure in the early stages of admission to help doctors adjust the management of community-acquired pneumonia (CAP) patients.Methods: We conducted a 30-day cohort study of newly admitted adult CAP patients over 20 years of age. Prognosis models to predict the short-term prognosis were developed using random survival forest (RSF) method.Results: A total of 247 adult CAP patients were studied and 208 (84.21%) of them reached clinical stability within 30 days. The soluble form of suppression of tumorigenicity-2 (sST2) was an independent predictor of clinical stability and the addition of sST2 to the prognosis model could improve the performance of the prognosis model. The C-index of the RSF model to predict clinical stability was 0.8342 (95%CI, 0.8086-0.8598), which is higher than 0.7181 (95%CI, 0.6933-0.7429) of CURB 65 score, 0.8025 (95%CI, 0.7776-8274) of PSI score and 0.8214(95% CI, 0.8080-0.8348) of cox regression. In addition, the RSF model was associated with adverse clinical events during hospitalization, ICU admissions and short-term mortality.Conclusion: The RSF model by incorporating sST2 was more accurate than traditional methods in assessing the short-term prognosis of CAP patients.
The clinical impact of IKZF1 mutation in acute myeloid leukemia
Authors: Zhang Xiang , Huang Aijie , Liu Lixia , Qin Jiayue , Wang Chengcheng , Yang Min , LOU Yinjun , Wang Lei , Ni Xiong , Hu Xiaoxia , Tang Gu-sheng , Zhang Mengmeng , Cao Shanbo , Mao Liping , Qian Jiejing , Xu Weilai , wei Ju-Ying , Xu Gaixiang , Meng Haitao , Mai Wenyuan , Yang Chunmei , Zhu Hong-Hu , Tong Hong , Yang Jianmin , Yu Wenjuan , Wang Jianmin , Jin Jie
Published: May 2022

The genetic heterogeneity generates great challenges in the understanding and treating acute myeloid leukemia (AML), while the knowledge about IKZF1 mutation in AML was extremely limited. Previously, we described the distribution pattern of IKZF1 mutation in AML, but its clinical impact remained undefined due to limited cases. Herein, we devoted to answer this question in one relatively large cohort. Among 530 newly diagnosed AML patients, we identified 28 IKZF1 mutations in 22 patients (22/530, 4.15%). IKZF1-mutated patients presented one young median morbid age (P=0.027) and the high frequency of mixed lineage leukemia involvement (P=0.012). In AML, IKZF1 mutation showed significant co-occurrences with CEBPA (P<0.001), SF3B1 (P=0.001), or CSF3R (P=0.008) mutation. Though IKZF1-mutated AML was more classified into the intermediate-risky group (P=0.005), it showed one inferior complete remission rate (P=0.041). Furthermore, AML with high variant allele frequency of IKZF1 mutation showed one relatively shorter overall survival duration (P=0.047), and it was one independent factor for the increased risk of death (hazard ratio, 3.14; 95% CI, 1.27-7.75; P=0.013). In subgroup analysis, our results showed that IKZF1 mutation conferred one poor therapeutic response and prognosis for SF3B1-mutated AML (P=0.002). Therefore, IKZF1 mutation exhibited one unique distribution mode and essential prognostic role in AML.
Tislelizumab in combination with gemcitabine plus cisplatin chemotherapy as first-line adjuvant treatment for locally advanced or metastatic bladder cancer: a retrospective study
Authors: Ren Xiang , Tian Yiqun , Wang Zhixian , Wang Jing , Li Xing , Yin Yisheng , Chen Ruibao , Zhan Ying , Zeng Xiaoyong
Published: May 2022

Background: Combining immune checkpoint inhibitors (ICIs) with chemotherapy can synergistically improve antitumor activity and are generally well tolerated. Recently, the efficacy and safety of combination therapy has been demonstrated for many cancers, including urothelial carcinomas. The aim of this retrospective pilot study was to evaluate the efficacy and safety of tislelizumab plus chemotherapy as first-line adjuvant treatment for locally advanced or metastatic bladder cancer.Methods: We conducted a retrospective analysis of 31 patients with locally advanced or metastatic bladder cancer from December 2020 to January 2022 with an Eastern Cooperative Oncology Group performance status (ECOG) of 0/1. Of the 31 patients, 14 patients received tislelizumab (200 mg i.v. every 3 weeks, Q3W) plus 21-day cycles of chemotherapy (gemcitabine, 1000 mg/m² i.v. on days 1 and 8 of each cycle + cisplatin, 70 mg/m² i.v. on day 2 of each cycle) (TGC) treatment and 17 patients received gemcitabine plus cisplatin chemotherapy (GC) treatment. All patients treated with bladder cytoreductive surgery and were treated for four 21-day cycles until disease progression or intolerable treatment-related adverse events (TRAEs). The objective progression-free survival (PFS), overall survival (OS), overall response rate (ORR), disease control rate (DCR), clinical benefit rate (CBR) and TRAEs were recorded and reviewed. Results: As of the cut-off date (March 25, 2022), PFS, OS, ORR, DCR, CBR and TRAEs were evaluated in 14 patients receiving combination therapy and 17 patients in the chemotherapy alone group. The median PFS was 36.0 [95% confidence interval (CI), 33.1-38.9] weeks in the TGC group and 29.0 (95% CI, 25.4-32.6) weeks in the GC group [hazard ratio (HR), 0.15 (95% CI, 0.04-0.55)]. In the GC group, the median OS was 48.0 (95% CI, 39.7-56.3) weeks; the median OS was not yet mature for the TGC group [HR, 0.26 (95% CI, 0.07-0.94)]. Treatment with TGC resulted in improved DCR (TGC, 71.4%; GC, 65.0%) and CBR (TGC, 64.3%; GC, 52.9%) compared with GC. However, although higher incidences of grade ≥3 TRAEs were observed with TGC compared with GC (35.7% versus 23.5%), the difference was not statistically significant (p = 0.47).Conclusion: This study suggested that TGC provided survivors of locally advanced or metastatic bladder cancer with encouraging antitumor activity and was generally well tolerated.
Impact of PD-L1 gene polymorphism and its interaction with cooking with solid fuel exposure upon susceptibility to tuberculosis
Authors: Tang Kun , Zhong Hua , Wang Qiaozhi , Li Zihao , Wu Chunli , An Rongjing , Lin Ying , Wang Jing , Wang Mian , Tan Hongzhuan , Chen Lizhang , Chen Mengshi
Published: May 2022

Given that PD-L1 is a crucial immune checkpoint in regulating T cell responses, the aim of this study was to explore the impact of PD-L1 gene polymorphism and its interaction with cooking with solid fuel on susceptibility to tuberculosis (TB) in Chinese Han populations. A total of 503 TB patients and 494 healthy controls were enrolled in this case-control study. Mass spectrometry (MS) technology was applied to genotype rs2297136 and rs4143815 of PD-L1 genes. The associations between SNPs and TB were assessed using unconditional logistic regression analysis. Marginal structural linear odds models were used to estimate the gene-environment interactions. Compared with genotype CC, genotypes GG and CG + GG at rs4143815 locus were significantly associated with susceptibility to TB (OR: 3.074 and 1.506, respectively, P < 0.05). However, no statistical association was found between rs2297136 SNP and TB risk. Moreover, the relative excess risk of interaction (RERI) between rs4143815 of the PD-L1 gene and cooking with solid fuel was 2.365 (95% CI 1.922,2.809), suggesting positive interactions with TB susceptibility. In conclusion, the rs4143815 polymorphism of the PD-L1 gene was associated with susceptibility to TB in Chinese Han populations. There were significantly positive interactions between rs4143815 and cooking with solid fuel.
Acneiform eruptions with combination targeted cancer therapy in colorectal cancer patients
Authors: Ibraheim Marina K , Lo Jonathan , Gupta Rohit , Parseghian Christine , Patel Anisha B
Published: May 2022

Purpose: Epidermal growth factor receptor inhibitors (EGFRI) can be used with pathway inhibitors, including mitogen-activated protein kinase kinase inhibitors (MEKIs), BRAF inhibitors (BRAFIs), and checkpoint inhibitors such as programmed death-ligand 1 (PD-L1) and programmed cell death protein 1 (PD-1) to treat colorectal cancer. These can precipitate treatment-resistant acneiform eruptions, prompting dose modification or discontinuation. Predicting the likelihood of severe rash development and crafting effective treatments may promote adherence to life-saving chemotherapy. Methods: An Institutional Review Board approved retrospective chart review of patients with colorectal cancer treated with EGFRI or MEKI in combination with HER2, BRAF, PI3K, or checkpoint inhibitors between January 1, 2016 and January 1, 2020 was performed. Surrogates for rash severity were investigated, including lower extremity involvement, utilization of oral steroids or retinoids, dose modification, and incidence of superinfection. Results: Of 122 patients treated with combination therapy, 105 developed a rash, and 87 developed an acneiform eruption. Common combinations included MEKI/PD-LI, EGFRI/MEKI, and MEKI/PD-1I. Patients treated with EGFRI/MEKI developed the most severe rashes (p=0.02). Lower extremity involvement was more frequent with EGFRI/MEKI compared to alternative combinations (p=0.05). Drug holiday correlated with all rash severity surrogates, including rash grade, lower extremity involvement, oral steroid or retinoid use, and incidence of superinfection. Use of oral steroids or retinoids was associated with development of superinfection (p=0.002). Prophylactic tetracycline use did not impact rash severity or rash incidence. Conclusion: This is the first descriptive analysis to characterize acneiform eruptions for patients with colorectal cancer on combination cancer therapy. Approximately 85% of patients developed a cutaneous toxicity with, what appears, to be synergistic effects of EGFRI and MEKI combination therapy causing the most severe eruptions. Superinfection rate correlated to systemic therapy use beyond oral tetracyclines. Further investigation into the utility of prophylactic oral tetracyclines in this population is needed.
Preparation of Environmentally Friendly Method of zinc oxide (ZnO) nanoparticles from osmium gratissimum (Damaakasee) plant leaf extracts and its antibacterial Activities
Authors: Saka Abel , Jule Leta Tesfaye , Gudata Lamessa , Seeivasan Venkatesh , N Nagaprasad , Ramaswamy Krishnaraj
Published: May 2022

Biological approach of Zinc oxide (ZnO) Nano particles by using Ocimum gratissimum (Damaakasee) leaf extracts were deposited and galvanized at three different heats. Zinc-nitrate hexa-hydrate (ZnNO3.6H2O) is used as metallic precursor source. The UV-Vis absorbance crests at 3oo nm specify a blue alteration in exciton preoccupation. Scanning electron microscope (SEM) micrographs demonstrates the Nano particles to be poly disseminated and a widespread assortment of magnitudes with slighter Nano particles grouping round greater micro-meter sized ones with shrill ends. A galvanizing hotness of 200 ℃ occasioned in the sintering of Nano particles nonetheless Nano particles galvanizing at 150 ℃ had leaky shells signifying a conceivable in radar tenders. An x-ray diffraction (XRD) array reveals that the Zinc Oxide Nano particles have the hexagonal wurzite configuration and of worthy crystal shape. Attained crystals sizes greater than before with annealing temperature and were in the range 18.66 nm to 27. 26 nm and average size 23.4 nm. The photo-luminescence (PL) continuums indicates (2) emanation crests. The photo-luminescence (PL) crowning strengths were reliant on the galvanizing temperatures with the UV- radiation crests for greater galvanizing temperatures demonstrating a phantom shift near lower wave lengths. The anti-bacterial activity of Zinc Oxide Nano particles gained from Ocimum gratissimum leafs against Escherichia coli and staphylococcus aureus were considered. The anti-bacterial bustle also displays virtuous zone of reserve. Green approach method encouraging for Synthesization of ZnO Nano particles in anti-bacterial bustle and illustrate the same result. This consequence validates that the biological prepared Nanoparticles initiate to have therapeutic applications and is biological degradable in microbial.
Search and Processing of Holliday Junctions within Long DNA by Junction-Resolving Enzymes
Authors: Kaczmarczyk Artur , Déclais Anne-Cécile , Newton Matthew , Boulton Simon , Lilley David , Rueda David
Published: May 2022

Resolution of four-way Holliday junctions is a critical intermediate step of homologous recombination. DNA junctions must be processed by abundant junction-resolving endonucleases to cleave the covalent link between two chromosomes. Although the catalytic activity and interaction mode of endonucleases with Holliday junction have been characterized in great detail by biochemical and structural studies, it remains unclear how the enzymes find their substrate located within kilobase pairs of duplex DNA. Here, we present a complete single-molecule reaction trajectory for a junction-resolving enzyme finding and cleaving a Holliday junction. We employed correlative optical tweezers with confocal fluorescence microscopy to track a single dimer of endonuclease I on the DNA template in real-time. We observed that the enzyme binds remotely to the dsDNA and then undergoes 1D diffusion. Upon encountering the four-way junction, a catalytically impaired endonuclease I mutant remains bound at that point for long periods. An active enzyme, however, cleaves the junction after a few seconds. When an N-terminal truncated endonuclease mutant is used, the enzyme fails to diffuse along dsDNA and dissociates after short periods of time, indicating that DNA encirclement by the disordered N-terminus is a key requirement for 1D diffusion and efficient target localisation. Quantitative analysis of each of these stages revealed a comprehensive description of the facilitated diffusion mechanism. We show that the eukaryotic junction-resolving enzyme GEN1 also undergoes facilitated diffusion on dsDNA until it becomes located at a junction, so that the general resolution trajectory is likely to be applicable to most junction resolving enzymes.
Luteolin-7-O-glucoside Corrects the Disorder of Glucose Metabolism in Treating Respiratory syncytial virus Pneumonia
Authors: Li Wei-Feng , Chen Yan-Zhen , Ling Xiao-Yin , Sun Ya-Lei , Yuan Bing , Tao Jia-Lei
Published: May 2022

Background
Respiratory syncytial virus (RSV) pneumonia is the main causes of acute bronchiolitis in infants. Luteolin-7-O-glucoside (LUT-7G) is a natural flavonoid, which exists in a variety of plants and has a variety of biological activities. The purpose of this study was to reveal the therapeutic effect of LUT-7G on RSV pneumonia from the perspective of glucose metabolism.
Methods
Mice model of RSV pneumonia were constructed and administered LUT-7G intervention (60mg/kg·d− 1). The pathological injury of lung tissue was observed by HE staining. Real-time qPCR was used to detect inflammatory factors and RSV surface protein. Then the metabolites of glycolysis and tricarboxylic acid cycle (TCA) in lung tissue and serum of mice were detected by gas chromatography-mass spectrometry (GC-MS) to evaluate the regulatory effect of LUT-7G on glucose metabolism under virus infection.
Results
Pharmacodynamic studies show that LUT-7G could reduce the inflammatory injury of lung tissue by up-regulating IL-10 mRNA, reducing RSV G, F protein and pro-inflammatory factor TNF-α and TGF-β mRNA. Target metabonomics showed that RSV infection could up-regulate the levels of glycolysis and TCA metabolites in lung tissue and serum, while LUT-7G could improve the disorder of glucose metabolism and partially reverse the upregulation of glucose, lactate and malate in lung tissue.
Conclusion
LUT-7G can improve the pathological injury of lung tissue induced by RSV by regulating glucose metabolism and inhibiting inflammation.
A coumarin-azo derived colorimetric chemosensor for Hg 2+ detection in organic and aqueous media and its extended real-world applications
Authors: Battison Aidan , Schoeman Stiaan , Mama Neliswa
Published: May 2022

Pollution caused by the release of toxic heavy metals into the environment by industrial and farming processes has been regarded as a major problem worldwide. This has attracted a great deal of attention into restoration and remediation. Mercury is classified as a toxic heavy metal which has posed significant challenges to public and environmental health. To date, conventional methods for mercury detection rely on expensive, destructive, complex, and highly specialized methods. Evidently, there is a need to develop systems capable of easily identifying and quantifying mercury within the environment. In this way, organic-based colorimetric chemosensors are gaining increasing popularity due to their high sensitivity, selectivity, cost-effectiveness, ease of design, naked-eye, and on-site detection ability. The formation of coumarin-azo derivative AD1 was carried out by a conventional diazotization reaction with coumarin-amine 1c and N,N-dimethylaniline. Sensor AD1 displayed remarkable visual colour change upon mercury addition with appreciable selectivity and sensitivity. The detection limit was calculated as 0.24 µM. Additionally, the reversible nature of AD1 allowed for the construction of an IMPLICATION type logic gate and Molecular Keypad Lock. Chemosensor AD1 displayed further sensing applications in real-world water samples and towards on-site assay methods. Herein, we describe a coumarin-derived chemosensor bearing an azo (N = N) functionality for the colorimetric and quantitative determination of Hg2+ in organic and aqueous media.
Phytochemicals Quantification, TLC and Antimicrobial Assessment of the Leaves and Fruit Extracts of Lasimorpha Senegalensis (Schott) Araceae
Authors: BUNU SAMUEL JACOB , Miediegha Oyeintonbara , Nwankwo Ogechukwu L. , Ozor Martins O.
Published: May 2022

Background: Lasimorpha senegalensis is a perennial herbaceous plant that grows in marshy places and produces a clump of leaves from a short, thick, strongly stoloniferous rhizome. It's been used to treat gonorrhea and dysentery, among other ailments. The leaves can also be used to treat coughs and uneasiness and agitation in bigger dosages. It's also given to pregnant women to help them deliver more quickly. The study aimed to conduct phytochemical screening and evaluate the antimicrobial properties of Lasimorpha senegalensis. The leaves and fruit parts Lasimorpha senegalensis (Schott) Araceae were collected at Korokorosei community in Bayelsa State, air-dried, pulverized and extracted using dichloromethane, ethanol, methanol and water. Flavonoids, saponins, tannins, ketones, and cardiac glycosides were all found to be present. Preliminary thin layer chromatographic screening of the extracts was also done, as well as the antibacterial evaluation of the different fractions using the agar diffusion technique.Results: The phytochemical screening for tannins, cardiac glycoside, ketones, and flavonoids were positive. Inhibition zones for the aqueous fraction at 62.5, 125, 250 and 500 mg/ml for E. coli was 32, 22, 18 and 11 mm respectively, while 8, 9, 10 and 14 mm for Pseudomnas aeruginosa. For the methanolic fraction, at the above concentrations, 10, 11, 14 and 14 nm was observed as zones of inhibition on Escherichia coli, 14, 19, 15, and 20 nm for Pseudomnas aeruginosa, and 19, 20, 20, and 20 nm for Staphylococcus aureus, respectively. Furthermore, in the dichloromethane fraction, 13, 20, 19, and 15 nm were observed as zones of inhibition on Escherichia coli, 8, 10, 15, and 15 nm for Pseudomnas aeruginosa, and 20, 15, 20, and 18 nm for Staphylococcus aureus, respectively.Conclusion: It is evident, from the results obtained that the leaf portion of the plants can be used in the treatment of infections caused by E. col, Pseudomonas aeruginosa, and Staphylococcus aureus. With the high amounts of flavonoids observed, it can possibly be employed as an antioxidant and a cardioprotective.
Developing and validating an assessment tool on Knowledge, Attitude, and Practice of Central Line- Associated Bloodstream Infection of ICU Nurses
Authors: Dang Fangping , Gao Jing , Ding Nannan , Zhai Tiantian , Li Huiju , Ran Xingwu
Published: May 2022

Background
Because limited assessment tool is available regarding knowledge, attitude, and practice (KAP) of Central-Line Associated Bloodstream Infection (CLABSI) of intensive care unit (ICU) nurses, we developed and validated an assessment tool on KAP of CLABSI of ICU Nurses.
Methods
Based on an extensive literature search, we initially developed this questionnaire. Pilot study were performed with the initial questionnaire. Then we evaluated the reliability and validity of the new questionnaire. We performed confirmatory factor analysis and exploratory factor analysis. The correlation coefficients were calculated. A test-retest was done to establish the reliability and to refine the items further.
Results
Initially, 54 items were formed in the questionnaire. The questionnaire was sent out twice and statistical analyses were made, reducing the number of items. We performed the pre-investigation and 255 participants responded to the questionnaire items. The overall content validity of the questionnaire was 0.95. The KMO value was 0.855, and the chi-square value of Bartlett's sphere test was 1479.609 (P˂0.05). After the principal component analysis extraction method and the maximum variance orthogonal rotation, four common factors were extracted, and the cumulative variance contribution rate was 60.052%. The knowledge, attitude, and practice dimensions and the overall Cronbach's coefficient of the questionnaire were 0.927, 0.778, 0.923, and 0.946, respectively. Two weeks later, the test-retest reliability was 0.931, 0.825, 0.934, and 0.945, respectively.
Conclusions
A assessment tool for ICU nurses about KAP of CLABSI was developed, which included three domains covering knowledge, attitude, and practice. The questionnaire’s validity, and reliability have been verified and can be used in clinical practice. The establishment of the tool will facilitate accurate monitoring for nursing managers to grasp the status quo about the KAP of CLABSI among ICU nurses so that nurses can be trained in a targeted manner.
Foveal morphology of retinal layers in 4 to 6-year-old children with history of retinopathy of prematurity: J-CREST study
Authors: Nishi Tomo , Ueda Tetsuo , Mizusawa Yuutaro , Shinomiya Kayo , Mitamura Yoshinori , Kimura Naoki , Gomi Fumi , Miki Akiko , Nakamura Makoto , Kinoshita Takamasa , Obata Shumpei , Ohji Masahito , Tsuji Takuya , Yoshida Shigeo , Ogata Nahoko
Published: May 2022

The most effective treatment for retinopathy of prematurity (ROP) is considered as the laser therapy. We compared the foveal morphology of the retina in eyes with a history of ROP with that of full-term children. This was a cross-sectional comparative study. Seventy-eight patients with a history of ROP, aged 4–6 years, were included. Among them, 45 underwent laser treatment for ROP. The clinical findings and retinal morphology in these patients were compared with that of 33 patients who had spontaneous regression of ROP and 30 age-matched full-term controls. All patients with ROP had 20/40 or better best-corrected visual acuity (BCVA). The foveal thickness was significantly thicker and foveal depression was significantly shallower in laser-treated ROP eyes than in regressed ROP eyes and controls. The outer nuclear layer was significantly thicker, and the inner segment (IS) of the photoreceptors and the inner retinal layer (IRL) were significantly longer in the laser-treated ROP eyes than that in the control eyes. In ROP patients and controls, BCVA was associated with the foveal depression, foveal depression was associated with the gestational age and IS thickness was associated with ROP stage. Our results suggest that prematurity and laser treatment affect the foveal morphology and BCVA.
Developing prediction models for short-term mortality after surgery for colorectal cancer using a Danish national quality assurance database
Authors: Bräuner Karoline Bendix , Rosen Andreas W. , Tsouchnika Adamantia , Walbech Julie S. , Gögenur Mikail , Lin Viviane A. , Clausen Johan S. R. , Gögenur Ismail
Published: May 2022

Purpose
The majority of colorectal cancer surgeries are performed electively, and treatment is often decided at the multidisciplinary team conference. Although the average 30-day mortality rate is low, there is substantial population heterogeneity from young, healthy patients to frail, elderly patients. The individual risk of surgery can vary widely, and tailoring treatment for colorectal cancer may lead to better outcomes. This requires risk prediction that is accurate and available prior to surgery.
Methods
Data from the Danish Colorectal Cancer Group database was transformed into the Observational Medical Outcomes Partnership Common Data Model. Models were developed to predict the risk of mortality within 30, 90, and 180 days after colorectal cancer surgery using only preoperative covariates. Several machine-learning models were trained, but due to superior performance, a Least Absolute Shrinkage and Selection Operator Logistic Regression was used for the final model. Performance was assessed with discrimination (area under the receiver operating characteristic and precision recall curve) and calibration measures (calibration-in-the-large, intercept, slope, and Brier score).
Results
The cohort contained 65.612 patients operated for colorectal cancer in the period from 2001 to 2019 in Denmark. The Least Absolute Shrinkage and Selection Operator model showed an area under the receiver operating characteristic for 30-, 90- and 180-day mortality after colorectal cancer surgery of 0.871 (95% CI: 0.86–0.882), 0.874 (95% CI: 0.864–0.882) and 0.876 (95% CI: 0.867–0.883) and calibration-in-the-large of 1.01, 0.98 and 1.01 respectively.
Conclusion
The postoperative short-term mortality prediction model showed excellent discrimination and calibration using only preoperative predictors.