bluuebunny/crossref_metadata_2025 huggingface dataset:
Establishing Babesia bovis-Free Tick Colony Following Treatment of the Host with Diminazene Aceturate (Berenil)
Authors: Tirosh-Levy Sharon , Roth Asael , Leibovich Binyamin , Fleiderovitz Ludmila , Frid Ohad , Yasur-Landau Daniel , Wolkomirskyi Ricardo , Mazuz Monica L.
Published: May 2021

Babesia bovis is a widely-spread tick-borne hemoparasite of cattle with major economic and animal welfare consequences. Rhipicephalus (Boophilus) annulatus is a one-host tick which transmits bovine babesiosis in the Middle East and Africa. Laboratory rearing of ixodid ticks is essential for the investigation on ticks or tick-borne diseases. Establishing a tick colony in the laboratory usually originates from ticks harvested in the field, which may be naturally infected with various pathogens. This especially applies to carriage of B. bovis as it is highly prevalent in endemic areas and is transmitted transovarially in ticks. Here, we describe the use of diminazene aceturate (Berenil) in order to establish laboratory colonies of Babesia-free R. annulatus, from ticks collected in the field. Ticks collected in the field were kept until oviposition and hatched larvae were introduced to naïve calves, which led to infection of the calves with B. bovis. Calves were then treated with diminazene aceturate several times until the engorged ticks dropped. The eggs and larvae collected from these ticks were parasite-free, as demonstrated both by infection of splenectomized calves and by PCR. This suggested protocol is a useful tool to create parasite-free tick colony and may, theoretically, also be beneficial to reduce parasite circulation in the field, although not recommended, as resistance to diamenizene aceturate might develop.
Microeukaryotic Communities on the Fruit of Gardenia thunbergia Thunb. with a Focus on Pathogenic Fungi
Authors: Steudel Bastian , Baijnath Himansu , Schwedt Thorben , Schmitt Armin Otto
Published: May 2021

Woody fruit which stay on ornamental plants for a long time may present a risk of infection to other organisms due to the presence of pathogens on their surface. We compared the microbe communities on the fruit surfaces of garden ornamental Gardenia thunbergia Thunb. with those on other surfaces in the study region. As Gardenia fruit contain antifungal substances, the focus of this study was on the fungal communities that exist thereon. We used Illumina sequencing to identify Amplicon Sequence Variants (ASV) of the internal transcribed spacer 2 (ITS2) of the ribosomal RNA. The microbial communities of the Gardenia fruit are distinct from the communities from the surrounding environments, indicating a specialized microhabitat. We employed clustering methods to position unidentified ASVs relative to known ASVs. We identified a total of 56 ASVs representing high risk fungal species as putative plant pathogens exclusively found on the fruit of Gardenia. Additionally, we found several ASVs representing putative animal or human pathogens. Those pathogens were distributed over distinct fungi clades. The infection risk of the high diversity of putative pathogens represented on the Gardenia fruit needs to be elucidated in further investigations.
Investigating the Circulation of Ebola Viruses in Bats during the Ebola Virus Disease Outbreaks in the Equateur and North Kivu Provinces of the Democratic Republic of Congo from 2018
Authors: Lacroix Audrey , Mbala Kingebeni Placide , Ndimbo Kumugo Simon Pierre , Lempu Guy , Butel Christelle , Serrano Laetitia , Vidal Nicole , Thaurignac Guillaume , Esteban Amandine , Mukadi Bamuleka Daniel , Likofata Jacques , Delaporte Eric , Muyembe Tamfum Jean-Jacques , Ayouba Ahidjo , Peeters Martine , Ahuka Mundeke Steve
Published: May 2021

With 12 of the 31 outbreaks, the Democratic Republic of Congo (DRC) is highly affected by Ebolavirus disease (EVD). To better understand the role of bats in the ecology of Ebola viruses, we conducted surveys in bats during two recent EVD outbreaks and in two areas with previous outbreaks. Dried blood spots were tested for antibodies to ebolaviruses and oral and rectal swabs were screened for the presence of filovirus using a broadly reactive semi-nested RT-PCR. Between 2018 and 2020, 892 (88.6%) frugivorous and 115 (11.4%) insectivorous bats were collected. Overall, 11/925 (1.2%) to 100/925 (10.8%) bats showed antibodies to at least one Ebolavirus antigen depending on the positivity criteria. Antibodies were detected in fruit bats from the four sites and from species previously documented to harbor Ebola antibodies or RNA. We tested for the first time a large number of bats during ongoing EVD outbreaks in DRC, but no viral RNA was detected in the 676 sampled bats. Our study illustrates the difficulty to document the role of bats as a source of Ebolaviruses as they might clear quickly the virus. Given the increasing frequency of EVD outbreaks, more studies on the animal reservoir are urgently needed.
Use of Recombinant Escherichia coli Strains in Immunofluorescence Assays for Melioidosis Diagnosis
Authors: Lantong Kanoknart , Songsri Jirarat , Wisessombat Sueptrakool , Mala Wanida , Prommachote Warinda , Senghoi Wilaiwan , Kotepui Manas , Kaewrakmuk Jedsada , Jiranantasak Treenate , Tuanyok Apichai , Klangbud Wiyada Kwanhian
Published: May 2021

Burkholderia pseudomallei is a Gram-negative bacterium and the causative agent of melioidosis in humans and animals in the tropics. The clinical manifestations of melioidosis are diverse, ranging from localized infections to whole-body sepsis. The effective serological method is crucial for the point-of-care diagnosis of melioidosis. The aim of this study was to develop indirect immunofluorescence assay (IFA)-based methods for detecting immunoglobulin G (IgG) antibodies in melioidosis patients. These methods use whole-cell antigens made from recombinant E. coli strains that express major B. pseudomallei antigens, including TssM, OmpH, AhpC, BimA, and Hcp1. A total of 271 serum samples from culture-confirmed melioidosis patients (n = 81), patients with other known infections (n = 70), and healthy donors (n = 120) were tested. Our study showed that the recombinant TssM strain had the highest performance, with 92.6% sensitivity, 100% specificity, 100% positive predictive value, 96.9% negative predictive value, 97.8% efficiency, 97.0% accuracy, and no cross-reactivity. The method agreement analysis based on k efficiency calculations showed that all five IFA methods perfectly agreed with the standard culturing method, while the traditional indirect hemagglutination (IHA) method moderately agreed with the culture. In summary, our investigations showed that the TssM-IFA method could be used for melioidosis diagnosis.
Metabolic Control by DNA Tumor Virus-Encoded Proteins
Authors: Prusinkiewicz Martin A. , Mymryk Joe S.
Published: May 2021

Viruses co-opt a multitude of host cell metabolic processes in order to meet the energy and substrate requirements for successful viral replication. However, due to their limited coding capacity, viruses must enact most, if not all, of these metabolic changes by influencing the function of available host cell regulatory proteins. Typically, certain viral proteins, some of which can function as viral oncoproteins, interact with these cellular regulatory proteins directly in order to effect changes in downstream metabolic pathways. This review highlights recent research into how four different DNA tumor viruses, namely human adenovirus, human papillomavirus, Epstein–Barr virus and Kaposi’s associated-sarcoma herpesvirus, can influence host cell metabolism through their interactions with either MYC, p53 or the pRb/E2F complex. Interestingly, some of these host cell regulators can be activated or inhibited by the same virus, depending on which viral oncoprotein is interacting with the regulatory protein. This review highlights how MYC, p53 and pRb/E2F regulate host cell metabolism, followed by an outline of how each of these DNA tumor viruses control their activities. Understanding how DNA tumor viruses regulate metabolism through viral oncoproteins could assist in the discovery or repurposing of metabolic inhibitors for antiviral therapy or treatment of virus-dependent cancers.
Molecular Detection of Tick-Borne Pathogens in Stray Dogs and Rhipicephalus sanguineus sensu lato Ticks from Bangkok, Thailand
Authors: Do Thom , Phoosangwalthong Pornkamol , Kamyingkird Ketsarin , Kengradomkij Chanya , Chimnoi Wissanuwat , Inpankaew Tawin
Published: May 2021

Canine tick-borne pathogens (CTBPs) such as Babesia vogeli, Ehrlichia canis, Anaplasma platys, Hepatozoon canis, and Mycoplasma haemocanis are important pathogens in dogs worldwide. Rhipicephalus sanguineus sensu lato, the main vector of several CTBPs, is the most common tick species found on dogs in Thailand. The present study identified CTBPs in dogs and ticks infested dogs. Samples (360 dog blood samples and 85 individual ticks) were collected from stray dogs residing in 37 temples from 24 districts in Bangkok and screened for CTBPs using molecular techniques. The most common CTBP found infecting dogs in this study was Ehrlichia canis (38.3%) followed by Mycoplasma haemocanis (34.2%), Hepatozoon canis (19.7%), Babesia vogeli (18.1%), and Anaplasma platys (13.9%), respectively. Furthermore, A. platys (22.4%) was the most common CTBP in ticks followed by M. haemocanis (18.8%), B. vogeli (9.4%), H. canis (5.9%), and E. canis (2.4%), respectively. The detection of CTBPs from the present study highlights the potential risk of infections that may occur in stray dogs and their ticks residing in Bangkok temples. These findings underline the importance of performing active surveys to understand the complexity of distributions of CTBPs in dogs and their ticks in Thailand.
In Vitro Synergistic Inhibition of HT-29 Proliferation and 2H-11 and HUVEC Tubulogenesis by Bacopaside I and II Is Associated with Ca2+ Flux and Loss of Plasma Membrane Integrity
Authors: Tomita Yoko , Smith Eric , Palethorpe Helen M. , Nakhjavani Maryam , Yeo Kenny K. L. , Townsend Amanda R. , Price Timothy J. , Yool Andrea J. , Hardingham Jennifer E.
Published: May 2021

We previously showed how triterpene saponin bacopaside (bac) II, purified from the medicinal herb Bacopa monnieri, induced cell death in colorectal cancer cell lines and reduced endothelial cell migration and tube formation, and further demonstrated a synergistic effect of a combination of bac I and bac II on the inhibition of breast cancer cell line growth. Here, we assessed the effects of bac I and II on the colorectal cancer HT-29 cell line, and mouse (2H-11) and human umbilical vein endothelial cell (HUVEC) lines, measuring outcomes including cell viability, proliferation, migration, tube formation, apoptosis, cytosolic Ca2+ levels and plasma membrane integrity. Combined bac I and II, each applied at concentrations below IC50 values, caused a synergistic reduction of the viability and proliferation of HT-29 and endothelial cells, and impaired the migration of HT-29 and tube formation of endothelial cells. A significant enhancement of apoptosis was induced only in HUVEC, although an increase in cytosolic Ca2+ was detected in all three cell lines. Plasma membrane integrity was compromised in 2H-11 and HUVEC, as determined by an increase in propidium iodide staining, which was preceded by Ca2+ flux. These in vitro findings support further research into the mechanisms of action of the combined compounds for potential clinical use.
Considerations to Be Taken When Carrying Out Medicinal Plant Research—What We Learn from an Insight into the IC50 Values, Bioavailability and Clinical Efficacy of Exemplary Anti-Inflammatory Herbal Components
Authors: Abdel-Tawab Mona
Published: May 2021

Medicinal plants represent a big reservoir for discovering new drugs against all kinds of diseases including inflammation. In spite the large number of promising anti-inflammatory plant extracts and isolated components, research on medicinal plants proves to be very difficult. Based on that background this review aims to provide a summarized insight into the hitherto known pharmacologically active concentrations, bioavailability, and clinical efficacy of boswellic acids, curcumin, quercetin and resveratrol. These examples have in common that the achieved plasma concentrations were found to be often far below the determined IC50 values in vitro. On the other hand demonstrated therapeutic effects suggest a necessity of rethinking our pharmacokinetic understanding. In this light this review discusses the value of plasma levels as pharmacokinetic surrogates in comparison to the more informative value of tissue concentrations. Furthermore the need for new methodological approaches is addressed like the application of combinatorial approaches for identifying and pharmacokinetic investigations of active multi-components. Also the physiological relevance of exemplary in vitro assays and absorption studies in cell-line based models is discussed. All these topics should be ideally considered to avoid inaccurate predictions for the efficacy of herbal components in vivo and to unlock the “black box” of herbal mixtures.
Novel Self-Nano-Emulsifying Drug Delivery Systems Containing Astaxanthin for Topical Skin Delivery
Authors: Ponto Thellie , Latter Gemma , Luna Giuseppe , Leite-Silva Vânia R. , Wright Anthony , Benson Heather A. E.
Published: May 2021

Astaxanthin (ASX) is a potent lipophilic antioxidant derived from the natural pigment that gives marine animals their distinctive red-orange colour and confers protection from ultraviolet radiation. Self nano-emulsifying drug delivery systems (SNEDDS) have been successfully developed and evaluated to increase the skin penetration of ASX and target its antioxidant and anti-inflammatory potential to the epidermis and dermis. SNEDDS were prepared using a low-temperature spontaneous emulsification method, and their physical characteristics, stability, antioxidant activity, and skin penetration were characterized. Terpenes (D-limonene, geraniol, and farnesol) were included in the SNEDDS formulations to evaluate their potential skin penetration enhancement. An HPLC assay was developed that allowed ASX recovery from skin tissues and quantification. All SNEDDS formulations had droplets in the 20 nm range, with low polydispersity. ASX stability over 28 days storage in light and dark conditions was improved and antioxidant activity was high. SNEDDS-L1 (no terpene) gave significantly increased ASX penetration to the stratum corneum (SC) and the epidermis-dermis-follicle region (E + D + F) compared to an ASX in oil solution and a commercial ASX facial serum product. The SNEDDS-containing D-limonene gave the highest ASX permeation enhancement, with 3.34- and 3.79-fold the amount in the SC and E + D + F, respectively, compared to a similar applied dose of ASX in oil. We concluded that SNEDDS provide an effective formulation strategy for enhanced skin penetration of a highly lipophilic molecule, and when applied to ASX, have the potential to provide topical formulations for UV protection, anti-aging, and inflammatory conditions of the skin.
Development and Validation of an HPLC–MS/MS Method for Pioglitazone from Nanocarriers Quantitation in Ex Vivo and In Vivo Ocular Tissues
Authors: Miralles-Cardiel Esther , Silva-Abreu Marcelle , Calpena Ana Cristina , Casals Isidre
Published: May 2021

Pioglitazone (PGZ) is an oral anti-hyperglycemic agent, belongs to the class of thiazolidinediones, and is used for the treatment of diabetes mellitus type 2. In recent years, its anti-inflammatory activity has also been demonstrated in the literature for different diseases, including ocular inflammatory processes. Additionally, this drug belongs to Class II of the Biopharmaceutical Classification System, i.e., slightly soluble and highly permeable. The main objective of this study was to validate a new analytical HPLC-MS/MS method to quantify free-PGZ and PGZ from polymeric NPs to conduct nanoparticle application studies loaded with this active ingredient to transport it within ocular tissues. An accurate, sensitive, selective, reproducible and high throughput HPLC-MS/MS method was validated to quantify PGZ in cornea, sclera, lens, aqueous humor, and vitreous humor. The chromatographic separation was achieved in 10 min on a Kinetex C18 column. Linear response of PGZ was observed over the range of 5–100 ng/mL. The recovery of free-PGZ or PGZ from NPs was in the range of 85–110% in all tissues and levels tested. The intra-day and inter-day precision were <5% and <10%, respectively. The extracts were shown to be stable in various experimental conditions in all matrices studied. The range of concentrations covered by this validation is 80–1600 µg/kg of PGZ in ocular tissues. It is concluded that this method can be applied to quantify PGZ for in vivo and ex vivo biodistribution studies related to the ocular administration of free-PGZ and PGZ from nanoparticles.
Population Pharmacokinetics of Primaquine in the Korean Population
Authors: Lee Woo-Yul , Chae Dong-Woo , Kim Choon-Ok , Lee Sang-Eun , Kwak Yee-Gyung , Yeom Joon-Sup , Park Kyung-Soo
Published: May 2021

While primaquine has long been used for malaria treatment, treatment failure is common. This study aims to develop a population pharmacokinetic model of primaquine and its metabolite, carboxyprimaquine, and examine factors influencing pharmacokinetic variability. The data was obtained from a clinical study in 24 Korean subjects randomly assigned to normal and obese groups. The participants received primaquine 15 mg daily for 4 days and blood samples were collected at day 4. Pharmacokinetic modeling was performed with NONMEM and using simulations; the influences of doses and covariates on drug exposure were examined. A minimal physiology-based pharmacokinetic model connected with a liver compartment comprehensively described the data, with CYP450 mediated clearance being positively correlated with the body weight and CYP2D6 activity score (p < 0.05). In the simulation, while the weight-normalized area under drug concentration for primaquine in the obese group decreased by 29% at the current recommended dose of 15 mg, it became similar to the normal weight group at a weight-normalized dose of 3.5 mg/kg. This study has demonstrated that the body weight and CYP2D6 activity score significantly influence the pharmacokinetics of primaquine. The developed model is expected to be used as a basis for optimal malaria treatment in Korean patients.
Targeting KRAS in Solid Tumors: Current Challenges and Future Opportunities of Novel KRAS Inhibitors
Authors: Indini Alice , Rijavec Erika , Ghidini Michele , Cortellini Alessio , Grossi Francesco
Published: May 2021

Activating mutations in RAS family proteins are found in ~25% of all human cancers. Different solid tumors are correlated with mutations in certain isoforms of RAS, with Kirsten RAS (KRAS) being the most frequently mutated isoform. Historically, KRAS has been acknowledged as “undruggable”, largely because the RAS proteins do not appear to present suitable pockets to which small inhibitory molecules can bind. However, this scenario has changed over the last years with the advent of novel KRAS inhibitors. In this review, we describe the role of KRAS mutation across different solid tumors, providing data on novel KRAS inhibitors currently under development and an updated overview of ongoing research in this field. A literature search was performed to select papers, abstracts, and oral presentation on KRAS inhibitory strategies in KRAS mutated solid tumors. Overall, the most promising therapeutic results have been obtained with molecules targeting KRAS G12C, thus paving the way for a significant therapeutic improvement in non-small cell lung cancer. Unfortunately, KRAS G12C mutation is rather uncommon in other solid tumors, namely pancreatic ductal adenocarcinoma and colorectal cancer. Several combination strategies are currently under evaluation in clinical trials, in order to bypass the resistance mechanisms responsible for the intrinsic resistance of mutated KRAS to the main therapeutic strategies adopted to date. Results suggest that the therapeutic scenario of KRAS has started to change, and further research will bring therapeutic results in this field.
Permeability of the Retina and RPE-Choroid-Sclera to Three Ophthalmic Drugs and the Associated Factors
Authors: Kim Hyeong Min , Han Hyounkoo , Hong Hye Kyoung , Park Ji Hyun , Park Kyu Hyung , Kim Hyuncheol , Woo Se Joon
Published: May 2021

In this study, Retina-RPE-Choroid-Sclera (RCS) and RPE-Choroid-Sclera (CS) were prepared by scraping them off neural retina, and using the Ussing chamber we measured the average time–concentration values in the acceptor chamber across five isolated rabbit tissues for each drug molecule. We determined the outward direction permeability of the RCS and CS and calculated the neural retina permeability. The permeability coefficients of RCS and CS were as follows: ganciclovir, 13.78 ± 5.82 and 23.22 ± 9.74; brimonidine, 15.34 ± 7.64 and 31.56 ± 12.46; bevacizumab, 0.0136 ± 0.0059 and 0.0612 ± 0.0264 (×10−6 cm/s). The calculated permeability coefficients of the neural retina were as follows: ganciclovir, 33.89 ± 12.64; brimonidine, 29.83 ± 11.58; bevacizumab, 0.0205 ± 0.0074 (×10−6 cm/s). Between brimonidine and ganciclovir, lipophilic brimonidine presented better RCS and CS permeability, whereas ganciclovir showed better calculated neural retinal permeability. The large molecular weight drug bevacizumab demonstrated a much lower permeability than brimonidine and ganciclovir. In conclusion, the ophthalmic drug permeability of RCS and CS is affected by the molecular weight and lipophilicity, and influences the intravitreal half-life.
Synthesis and In Vivo Evaluation of Insulin-Loaded Whey Beads as an Oral Peptide Delivery System
Authors: Heade Joanne , McCartney Fiona , Chenlo Miguel , Marro Olga Moreno , Severic Maja , Kent Robert , Bleiel Sinead B. , Alvarez Clara V. , Griffin Brendan T. , Brayden David J.
Published: May 2021

For many diabetics, daily, lifelong insulin injections are required to effectively manage blood glucose levels and the complications associated with the disease. This can be a burden and reduces patient quality of life. Our goal was to develop a more convenient oral delivery system that may be suitable for insulin and other peptides. Insulin was entrapped in 1.5-mm beads made from denatured whey protein isolate (dWPI) using gelation. Beads were then air-dried with fumed silica, Aerosil®. The encapsulation efficiency was ~61% and the insulin loading was ~25 µg/mg. Dissolution in simulated gastric-, and simulated intestinal fluids (SGF, SIF) showed that ~50% of the insulin was released from beads in SGF, followed by an additional ~10% release in SIF. The omission of Aerosil® allowed greater insulin release, suggesting that it formed a barrier on the bead surface. Circular dichroism analysis of bead-released insulin revealed an unaltered secondary structure, and insulin bioactivity was retained in HepG2 cells transfected to assess activation of the endogenous insulin receptors. Insulin-entrapped beads were found to provide partial protection against pancreatin for at least 60 min. A prototype bead construct was then synthesised using an encapsulator system and tested in vivo using a rat intestinal instillation bioassay. It was found that 50 IU/kg of entrapped insulin reduced plasma glucose levels by 55% in 60 min, similar to that induced by subcutaneously (s.c.)-administered insulin (1 IU/kg). The instilled insulin-entrapped beads produced a relative bioavailability of 2.2%. In conclusion, when optimised, dWPI-based beads may have potential as an oral peptide delivery system.
Twin-Screw Melt Granulation for Oral Solid Pharmaceutical Products
Authors: Forster Seth P. , Dippold Erin , Chiang Tiffany
Published: May 2021

This article highlights the advantages of pharmaceutical continuous melt granulation by twin-screw extrusion. The different melt granulation process options and excipients are described and compared, and a case is made for expanded use of twin-screw melt granulation since it is a flexible and continuous process. Methods for binder selection are profiled with a focus on rheology and physical stability impacts. For twin-screw melt granulation, the mechanism of granulation and process impact on granule properties are described. Pharmaceutical applications of melt granulation ranging from immediate release of soluble and insoluble APIs, taste-masking, and sustained release formulation are reviewed, demonstrating the range of possibilities afforded by twin-screw melt granulation.
The Biological Effects of Novel Nutraceuticals with Curcuminoids and Other Plant-Derived Immunomodulators and Pre-Probiotics
Authors: Răducanu Adina Elena , Tihăuan Bianca-Maria , Marinaș Ioana Cristina , Ciupercă Oana Teodora , Țebrencu Carmen Elena , Ionescu Elena , Onisei Tatiana
Published: May 2021

An effective and well-balanced immune system is pivotal for maintaining health. Diet and nutrition can affect the functioning of numerous immune parameters, with direct repercussions on homeostasis. Since our immune functions are indispensable in defending the body against pathogens and thus play a vital role in maintaining health, modulating immune response may well serve as the basis for the development of plant-based functional foods and novel nutraceuticals. This concept is currently utilized in attempts to prevent or mitigate inflammatory reactions via the development of targeted food products or active ingredients since an extended number of phytoconstituents (such as curcuminoids) are associated with beneficial effects on immunity. Immunomodulatory plant-based dietary supplements are considered effective in improving immune functions and reducing the incidence of immunological disorders or imbalances. Therefore, the main focus of this study was to evidence the beneficial biological effects such as antioxidant and antimicrobial, as well as nutritional status, biocompatibility and cell proliferation capacity and immunomodulation of two novel nutraceuticals. The first nutraceutic was based on curcuminoids and other actives from Trigonella foenum- graecum (seeds), Chelidonium majus L. (aerial parts), Taraxacum officinale L. (roots), vitamins (C, D3, A, E) and minerals (zinc) whereas the second one was made of probiotics such as Lactobacillus acidophilus and Bifidobacterium animalis subsp. Lactis combined with actives from Helianthus tuberosus (tubers) and Psyllium/Plantago ovata (husk) as herbal prebiotics.
Electron Spill-Out Effect in Singular Metasurfaces
Authors: Yang Fan , Ding Kun
Published: May 2021

The electron spill-out effect is considered in a singular metasurface. Using the hydrodynamic model, we found that electron spill-out effectively smears the sharp singularity. The introduction of the electron spill-out effect also significantly changes the reflection spectrum, charge distribution, field profile for a singular metasurface. Therefore, this spill-out contribution is crucial and cannot be ignored for a realistic description of optical response in a singular system.
Molecular Characterization of the Coat Protein Gene of Greek Apple Stem Pitting Virus Isolates: Evolution through Deletions, Insertions, and Recombination Events
Authors: Mathioudakis Matthaios M. , Maliogka Varvara I. , Candresse Thierry , Nickel Osmar , Fajardo Thor Vinicius Martins , Budzyńska Daria , Hasiów-Jaroszewska Beata , Katis Nikolaos I.
Published: May 2021

A RT–PCR assay developed to amplify the full coat protein (CP) gene of apple stem pitting virus (ASPV) was evaluated using 180 Greek apple and pear samples and showed a broad detection range. This method was used to investigate the presence of ASPV in quince in Greece and showed a high incidence of 52%. The sequences of 14 isolates from various hosts with a distinct RFLP profile were determined. ASPV population genetics and the factors driving ASPV evolution were analyzed using the Greek ASPV sequences, novel sequences from Brazilian apple trees and Chinese botanical Pyrus species, and homologous sequences retrieved from GenBank. Fourteen variant types of Greek, Brazilian and botanical isolates, which differ in CP gene length and presence of indels, were identified. In addition, these analyses showed high intra- and inter-group variation among isolates from different countries and hosts, indicating the significant variability present in ASPV. Recombination events were detected in four isolates originating from Greek pear and quince and two from Brazilian apples. In a phylogenetic analysis, there was a tendency for isolates to cluster together based on CP gene length, the isolation host, and the detection method applied. Although there was no strict clustering based on geographical origin, most isolates from a given country tended to regroup in specific clusters. Interestingly, it was found that the phylogeny was correlated to the type, position, and pattern of indels, which represent hallmarks of specific lineages and indicate their possible role in virus diversification, rather than the CP size itself. Evidence of recombination between isolates from botanical and cultivated species and the clustering of isolates from botanical species and isolates from cultivated species suggest the existence of a possible undetermined transmission mechanism allowing the exchange of ASPV isolates between the cultivated and wild/ornamental hosts.
Phospholipases C and D and Their Role in Biotic and Abiotic Stresses
Authors: González-Mendoza Víctor M. , Sánchez-Sandoval M. E. , Castro-Concha Lizbeth A. , Hernández-Sotomayor S. M. Teresa
Published: May 2021

Plants, as sessile organisms, have adapted a fine sensing system to monitor environmental changes, therefore allowing the regulation of their responses. As the interaction between plants and environmental changes begins at the surface, these changes are detected by components in the plasma membrane, where a molecule receptor generates a lipid signaling cascade via enzymes, such as phospholipases (PLs). Phospholipids are the key structural components of plasma membranes and signaling cascades. They exist in a wide range of species and in different proportions, with conversion processes that involve hydrophilic enzymes, such as phospholipase-C (PLC), phospholipase-D (PLD), and phospholipase-A (PLA). Hence, it is suggested that PLC and PLD are highly conserved, compared to their homologous genes, and have formed clusters during their adaptive history. Additionally, they generate responses to different functions in accordance with their protein structure, which should be reflected in specific signal transduction responses to environmental stress conditions, including innate immune responses. This review summarizes the phospholipid systems associated with signaling pathways and the innate immune response.
Erratum: Despinasse et al. Structure of the Chemical and Genetic Diversity of the True Lavender over Its Natural Range. Plants 2020, 9, 1640
Authors: Despinasse Yolande , Moja Sandrine , Soler Catherine , Jullien Frédéric , Pasquier Bernard , Bessière Jean-Marie , Baudino Sylvie , Nicolè Florence
Published: May 2021

As requested by the Editorial Office, the authors remove the scientific consortium “Camille Nous” from the author list and the Author Contributions section in the published paper [...]
Use of Grape Peels By-Product for Wheat Pasta Manufacturing
Authors: Iuga Mădălina , Mironeasa Silvia
Published: May 2021

Grape peels (GP) use in pasta formulation represents an economic and eco-friendly way to create value-added products with multiple nutritional benefits. This study aimed to evaluate the effect of the GP by-product on common wheat flour (Triticum aestivum), dough and pasta properties in order to achieve the optimal level that can be incorporated. Response surface methodology (RSM) was performed taking into account the influence of GP level on flour viscosity, dough cohesiveness and complex modulus, pasta color, fracturability, chewiness, cooking loss, total polyphenols, dietary fibers and resistant starch amounts. The result show that 4.62% GP can be added to wheat flour to obtain higher total polyphenols, resistant starch and dietary fiber contents with minimum negative effects on pasta quality. Flour viscosity, dough cohesiveness, complex modulus and pasta fracturability of the optimal sample were higher compared to the control, while chewiness was lower. Proteins’ secondary structures were influenced by GP addition, while starch was not affected. Smooth starch grains embedded in a compact protein structure containing GP fiber was observed. These results show that GP can be successfully incorporated in wheat pasta, offering nutritional benefits by their antioxidants and fiber contents, without many negative effects on the final product’s properties.
Strategic Possibility Routes of Recycled PET
Authors: Damayanti  , Wu Ho-Shing
Published: May 2021

The polyethylene terephthalate (PET) application has many challenges and potential due to its sustainability. The conventional PET degradation was developed for several technologies to get higher yield products of ethylene glycol, bis(2-hydroxyethyl terephthalate) and terephthalic acid. The chemical recycling of PET is reviewed, such as pyrolysis, hydrolysis, methanolysis, glycolysis, ionic-liquid, phase-transfer catalysis and combination of glycolysis–hydrolysis, glycolysis–methanolysis and methanolysis–hydrolysis. Furthermore, the reaction kinetics and reaction conditions were investigated both theoretically and experimentally. The recycling of PET is to solve environmental problems and find another source of raw material for petrochemical products and energy.
Engineering Toolbox for Systematic Design of PolyHIPE Architecture
Authors: Dhavalikar Prachi , Shenoi Jason , Salhadar Karim , Chwatko Malgorzata , Rodriguez-Rivera Gabriel , Cheshire Joy , Foudazi Reza , Cosgriff-Hernandez Elizabeth
Published: May 2021

Polymerization of high internal phase emulsions (polyHIPEs) is a well-established method for the production of high porosity foams. Researchers are often regulated to using a time-intensive trial and error approach to achieve target pore architectures. In this work, we performed a systematic study to identify the relative effects of common emulsion parameters on pore architecture (mixing speed, surfactant concentration, organic phase viscosity, molecular hydrophobicity). Across different macromer chemistries, the largest magnitude of change in pore size was observed across surfactant concentration (~6 fold, 5–20 wt%), whereas changing mixing speeds (~4 fold, 500–2000 RPM) displayed a reduced effect. Furthermore, it was observed that organic phase viscosity had a marked effect on pore size (~4 fold, 6–170 cP) with no clear trend observed with molecular hydrophobicity in this range (logP = 1.9–4.4). The efficacy of 1,4-butanedithiol as a reactive diluent was demonstrated and provides a means to reduce organic phase viscosity and increase pore size without affecting polymer fraction of the resulting foam. Overall, this systematic study of the microarchitectural effects of these macromers and processing variables provides a framework for the rational design of polyHIPE architectures that can be used to accelerate design and meet application needs across many sectors.
Simulation of Mode I Interlaminar Damage of a GFRP Composite Using Cohesive Laws in the Framework of the Equivalent LEFM R-Curve and an Optimised Algorithm
Authors: Torres Luis , Saavedra Karin , Pincheira Gonzalo , Pina Juan Carlos
Published: May 2021

This paper is focused on mode I delimitation of a unidirectional glass fibre reinforced polymer (GFRP) composite. The aim is to propose an accurate and simple characterisation of three cohesive zone models (CZM)—bilinear, trilinear, and potential—from the measurement of the load-displacement curve during a double cantilever beam experimental test. For that, a framework based on the equivalent linear elastic fracture mechanics (LEFM) R-curve is here proposed, which has never before been developed for a bilinear and a potential CZM. Besides, in order to validate this strategy, an optimisation algorithm for solving an inverse problem is also implemented. It is shown that the parameters’ identification using the equivalent LEFM R-curve enables the same accuracy but reduces 72% the numerical efforts respect to a “blind fitting” (i.e., the optimisation algorithm). Therefore, even if optimisation techniques become popular at present due to their easy numerical implementation, strategies founded on physical models are still better solutions especially when evaluating the objective function is expensive as in mechanical problems.
Investigating the Material Properties and Microstructural Changes of Fused Filament Fabricated PLA and Tough-PLA Parts
Authors: Naveed Nida
Published: May 2021

Fused deposition modelling (FDM) is a popular but complex additive manufacturing process that works with many process parameters which are crucial to investigate. In this study, 3D parts were fabricated by placing each filament layer in opposite direction to the others; for this, two combinations of raster angles, (45° −45°) and (0° 90°), along with three different infill speeds were used. In this study, two 3D printing material types—Polylactic Acid (PLA) and tough-PLA were used. The material properties of each 3D part were investigated to identify the best combination of these parameters. A microstructural analysis was also performed on outer and inner surfaces along with fracture interface of the parts after tensile testing using a scanning-electron-microscopy (SEM) to explain material failure modes and reasons. The results suggest that for both the material types, a raster angle of 45° −45° produces stronger parts than to a raster angle of 0° 90°. This study also suggests that a slow infill speed improves tensile properties by providing a better inner-connection between two contiguous roasters. Thus, the detailed analysis of microstructural defects correlated with tensile test results provides insight into the optimisation of raster angle and infill speed, and scope for improvement of mechanical properties.
Electrochemical Dopamine Biosensor Based on Poly(3-aminobenzylamine) Layer-by-Layer Self-Assembled Multilayer Thin Film
Authors: Panapimonlawat Tayanee , Phanichphant Sukon , Sriwichai Saengrawee
Published: May 2021

Dopamine (DA) is an important neurotransmitter which indicates the risk of several neurological diseases. The selective determination with low detection limit is necessary for early diagnosis and prevention of neurological diseases associated with abnormal concentration of DA. The purpose of this study is to fabricate a poly(3-aminobenzylamine)/poly(sodium 4-styrenesulfonate) (PABA/PSS) multilayer thin film for use as an electrochemical DA biosensor. The PABA was firstly synthesized using a chemical oxidation method of 3-aminobenzylamine (ABA) monomer with ammonium persulfate (APS) as an oxidant. For electrochemical biosensor, the PABA/PSS thin film was fabricated on fluorine doped tin oxide (FTO)-coated glass substrate using the layer-by-layer (LBL) self-assembly method. The optimized number of bilayers was achieved using SEM and cyclic voltammetry (CV) results. The electroactivity of the optimized LBL thin film toward detection of DA in neutral solution was studied by CV and amperometry. The PABA/PSS thin film showed good sensitivity for DA sensing with sensitivity of 6.922 nA·cm−2·µM−1 and linear range of 0.1–1.0 µM (R2 = 0.9934), with low detection limit of 0.0628 µM, long-term stability and good reproducibility. In addition, the selectivity of the PABA/PSS thin film for detection of DA under the common interferences (i.e., ascorbic acid, uric acid and glucose) was also presented. The prepared PABA/PSS thin film showed the powerful efficiency for future use as DA biosensor in real sample analysis.
Dyeing Para-Aramid Textiles Pretreated with Soybean Oil and Nonthermal Plasma Using Cationic Dye
Authors: Morris Mary , Ye Xiaofei Philip , Doona Christopher J.
Published: May 2021

The increasing use of functional aramids in a wide array of applications and the inert nature of aramids against conventional dye and print methods requires developing new dyeing methods. This study aims to use environmentally friendly method with a cationic dye as an alternative for dyeing para-aramid fabrics. Experiments used a multi-factorial design with functions of pretreatment, dye solvent (water and/or glycerol) and auxiliary chemical additives (swelling agent and surfactant) and a sequential experimentation methodology. The most effective dyeing procedures involved the following steps: (i) pretreatments of the fabrics with soybean oil and nonthermal plasma (NTP), (ii) using water at T = 100 °C as the dye solvent, and (iii) omitting other chemical additives. With a commercial cationic dye, these conditions achieved a color strength in K/S value of 2.28, compared to ~1 for untreated samples. FTIR analysis revealed that a functional network formed on the fibers and yarns of the fabrics by chemical reactions of excited plasma species with double bonds in the soybean oil molecules was responsible for significantly improving the color strength. These results extend the potential uses of a renewable material (soybean oil) and an environmentally friendly technology (NTP) to improve the dyeing of para-aramid textiles and reduce the use of harsh dye chemicals.
A Similarity Model of the Cooling Process of Fluids during Transportation
Authors: Brestovič Tomáš , Čarnogurská Mária , Příhoda Miroslav , Lázár Marián , Pyszko René , Jasminská Natália
Published: May 2021

This article presents a description of a novel method for the identification of a decrease in the temperature of a liquid medium transported by railroad tank cars. No exact analytical solution exists for this phenomenon; therefore, the authors of this article have prepared a mathematical expression for the cooling process of the transported fluid by applying a dimensional analysis, which facilitated the identification of the dimensionless criteria using the relevant dimensional parameters. A functional dependence between the criteria can be identified through a physical or numerical experiment. In this case, a database of the results from a detailed numerical model was used; however, its disadvantage is that the calculation takes much longer than in a simpler similarity model. The output of the similarity model was a function of the average temperature of the fluid at a time applicable to various alternatives in the geometrical, physical, and boundary conditions. The standard deviation of the difference between the temperatures predicted by the similarity model and those calculated by the numerical simulation Tmod − Tsim represented 4.8% relative to the simulated fluid temperature.
Evaluation of the Spoilage-Related Bacterial Profiles of Vacuum-Packaged Chilled Ostrich Meat by Next-Generation DNA Sequencing Approach
Authors: Juszczuk-Kubiak Edyta , Dekowska Agnieszka , Sokołowska Barbara , Połaska Marzena , Lendzion Krzysztof
Published: May 2021

Monitoring the development of the bacterial community in packaged raw meat refrigerated until two weeks is important for identifying the spoilage-related bacteria, preventing meat putrefaction, and prolong the shelf life. This study aimed to evaluate the influence of vacuum-packaging (VP) on the development of spoilage-related bacterial profiles in chilled ostrich meat among three manufacturing batches produced in different periods by using culture-dependent and 16S rDNA amplicon sequencing. Similar to the culture-dependent method, 16S rDNA sequencing showed that Photobacterium was the most prevalent genus detected in VP ostrich meat after 14 days of cold storage. The second-largest group was the population of lactic acid bacteria (LAB), mainly dominated by Carnobacteriaceae including Carnobacterium spp. and Lactobacillaceae with Lactobacillus spp. Our results suggest that these taxa could contribute to spoilage of VP ostrich meat and shorten its shelf life, especially Photobacterium spp., which is considered as a potential meat spoiler.
Development of an Impregnation End-Effector with Fiber Tension Monitoring for Robotic Coreless Filament Winding
Authors: Mindermann Pascal , Bodea Serban , Menges Achim , Gresser Götz T.
Published: May 2021

The manufacturing process of robotic coreless filament winding has great potential for efficient material usage and automation for long-span lightweight construction applications. Design methods and quality control rely on an adequate digital representation of the fabrication parameters. The most influencing parameters are related to the resin impregnation of the fibers and the applied fiber tension during winding. The end-effector developed in this study allows efficient resin impregnation, which is controlled online by monitoring the induced fiber tension. The textile equipment was fully integrated into an upscaled nine-axis robotic winding setup. The cyber-physical fabrication method was verified with an application-oriented large-scale proof-of-concept demonstrator. From the subsequent analysis of the obtained datasets, a characteristic pattern in the winding process parameters was identified.
Time-Dependent Viscous Flow Behavior of a Hydrophobic Fumed Silica Suspension
Authors: Sánchez Jorge , Rubio-Hernández Francisco , Páez-Flor Nicolás
Published: May 2021

The viscous flow behavior of a 12.5 vol% hydrophobic fumed silica (Aerosil® R816) suspension in polypropylene glycol of low molecular weight (PPG400) was studied in a stress-controlled rheometer. The steady flow curve showed shear thickening between two shear thinning regions. Time-dependent viscosity response provoked by step changes in shear stresses corresponding to the shear-thickening region apparently agrees with thixotropic behavior just after the very initial stages are surpassed. Almost instantaneous jamming can justify misinterpretation of the results.
Fabrication and Characterization of Hydrophobic Porous Metallic Membranes for High Temperature Applications
Authors: Claramunt Sara , Khurram Muhammad , Benzinger Walther , Kraut Manfred , Dittmeyer Roland
Published: May 2021

Hydrophobic porous metallic membranes can be integrated in a microreactor for in situ separation of steam at high temperatures. This study investigates the fabrication and characterization of hydrophobic coatings on metallic substrates. Two different coating methods were explored: (1) Plasma Enhanced—Chemical Vapor Deposition (PE-CVD) to form amorphous carbon silicon-doped a-C:H:Si:O thin films and (2) Direct Immersion in fluoroalkyl silane (FAS-13) solution using dip coating to form Self-Assembled Monolayers. The results on wettability as well as SEM images and EDS/WDS analyses indicate that the coated sintered stainless steel membranes are adequate as hydrophobic surfaces, maintaining the porosity of the substrate and withstanding high temperatures. Especially the FAS-13 coating shows very good resistance to temperatures higher than 250 °C. These findings are of special significance for the fabrication of porous metal membranes for separation of steam in high temperature applications.
The Comparison of the Efficacy of Natural and Synthetic Aluminosilicates, Including Zeolites, in Concurrent Elimination of Lead and Copper from Multi-Component Aqueous Solutions
Authors: Kozera-Sucharda Bożena , Gworek Barbara , Kondzielski Igor , Chojnicki Józef
Published: May 2021

The unique and outstanding physical and chemical properties of aluminosilicate minerals, including zeolites, make them extremely useful in remediation processes. That is due to their demonstrated high efficiency, inexpensiveness, and environmental friendliness in processes aimed on the elimination of heavy metals from water. The paper reports the results of the examination of selectivity of the tested clay minerals and zeolites toward different heavy metals in light of the postulated sorption mechanisms. It was stated that while the most efficient at concurrent removal of lead and copper from aqueous solutions were synthetic zeolites 3A and 10A, smectite was the best in dealing with prolonged pollution with Pb2+ and Cu2+. Determined as one of the parameters in DKR isotherm energy of the process for each combination of sorbate and sorbent, it showed that the dominant mechanism of adsorption on the tested mineral sorbents was physisorption. The exception was kaolinite, for which that energy implied ion exchange as the dominant mechanism of the process.