bluuebunny/crossref_metadata_2025 huggingface dataset:
Administración de la educación primaria: efectos percibidos de calidad y satisfacción de los padres de familia
Authors: Urquizo-Villamarín Adela , Pazmiño-Medina Diana
Published: December 2022

Cuando se trata de sectores educativos, el factor más importante a considerar es la calidad del servicio brindado por las instituciones, dado que la calidad del servicio conducirá a la satisfacción de los estudiantes y de sus representantes. Por ello, el objetivo del trabajo de investigación es identificar los efectos de calidad y satisfacción de los padres de familia con respecto a la administración de la educación primaria. De este modo, se aplicó el modelo HedPERF. La encuesta fue realizada a 60 padres de familia de los estudiantes de educación inicial, 2do y 5to de educación básica de la Unidad Educativa Julio Enrique Fernández ciudad de Ambato utilizando un método estadístico de análisis factorial exploratorio. Conforme con la formulación de hipótesis todas fueron aceptadas. Finalmente, se concluyó que, para mejorar la satisfacción en la calidad de la administración educativa, los administrativos deben tomar medidas de calidad como mejorar la imagen institucional, la resolución de problemas en programas académicos como instruir desde muy pequeños a los niños sobre distintas especialidades en las que se pueden interesar, y del mismo modo, si estos aspectos logran ser mejorados la lealtad de los representantes incrementará.
Ectogenesis and the Right to Life
Authors: Singh Prabhpal
Published: December 2022

In this discussion note on Michal Pruski and Richard C. Playford’s “Artificial Wombs, Thomson and Abortion – What Might Change?,” I consider whether the prospect of ectogenesis technology would make abortion impermissible. I argue that a Thomson-style defense may not become inapplicable due to the right to life being conceived as a negative right. Further, if Thomson-style defenses do become inapplicable, those who claim that ectogenesis would be an obligatory alternative to abortion cannot do so without first showing that fetuses have a right to life, something that Thomson assumed rather than argued for. I also include a discussion on ethical problems concerning what to do about children born from artificial wombs put there by those who looked to terminate their pregnancies because they sought to avoid parenthood.
Introduction of Multidisciplinary Team Approach for Head and Neck Cancer: Patient Satisfaction
Authors: Park Min Kyu , Song Chang Myeon , Park Hae jin , Choi Yoon-Young , Lee Young-Jun , Myung Jae Kyung , Oh Suk Joong , Park Seong Oh , Seo Hye Young , Lee Jin Won , Ji Yong-Bae , Tae Kyung
Published: December 2022

Background and Objectives The head and neck multidisciplinary team (MDT) approach plays a crucial role in bringing together the ideas of various medical professionals. This study aimed to evaluate the early characteristics of the MDT approach for head and neck cancer and analyzed patients’ satisfaction.Subjects and Method We analyzed 450 head and neck cancer patients who received MDT care from August 2014 to June 2022. Patient satisfaction with MDT care was evaluated by selfadministered questionnaires consisting of 9 questions.Results Of 450, 298 (66.2%) were male and 152 (33.8%) were female. The mean age was 60.8±14.7 year. The most common primary site was the larynx (17.3%), followed by the oral cavity and oropharynx. A total of 726 cases of the MDT approach were performed in 266 MDT sessions, and the mean number of patients per MDT session was 2.74. The number of medical professionals participating in MDT ranged from a minimum of 3 to a maximum of 9, with a mean of 5.11. The mean running time of MDT meetings per case was 19.51 minutes. The time of the 2nd MDT was significantly shorter than that of the 1st or 3rd MDT. The mean score was close to very satisfactory in each of the 9 patient satisfaction questions.Conclusion We believe that the MDT approach is feasible and recommend its introduction for the treatment of head and neck cancer as most patients have shown very high satisfaction. Further studies on the role and efficacy of MDT care for head and neck cancer are necessary.
The Jibril Method Implementasi Metode Jibril sebagai Upaya Memperbaiki Pelafalan Huruf-Huruf Hijaiyah dalam Konteks Membaca Al-Qur’an di Kelas 5 dan 6 MDA Al-Hidayah Desa Cikopo
Authors: Abdillah Diki Muhammad , Wulandari Dyah
Published: December 2022

This study aims to improve the pronunciation of the hijaiyah letters of the students when reading the Qur'an by using the Jibril method as a learning method. This study used a Participatory Action Research (PAR) design. The subjects in this study were students in grades 5 and 6 of MDA Al-Hidayah, Cikopo Village, totaling 11 people. First, the researcher conducted pre-action observations to determine the students' ability to pronounce the observed hijaiyah letters. Secondresearchers carry out actions in the form of implementing learning using the Jibril method. The implementation of actions in each cycle includes planning activities, implementing actions, carrying out observations, evaluations and reflections in accordance with the initial planning. Researchers used two cycles through post-test and reflection at the end of each cycle.The result of this study is that in the end the students were able to improve the pronunciation of the hijaiyah letters well. So that the Jibril method is a learning method that can improve the students' ability to pronounce the hijaiyah letters when reading the Qur'an.
Eksploitasi Digital Marketing Sebagai Usaha Meningkatkan Pendapatan Di Masa Pandemi Covid-19 Pada Pelaku UMKM Tanaman Hias Hafiz Flower
Authors: Nafsiah Sitti Nurhayati , Agustina Merry , Sahfitri Vivi , Marlindawati Marlindawati
Published: December 2022

Pandemi covid-19 membawa dampak yang cukup besar disegala aspek kehidupan baik sosial, budaya, pendidikan dan ekonomi. Begitu juga dengan para pelaku UMKM. Tak terkecuali kegiatan penjualan tanaman hias pada usaha  tanaman hias ”Hafiz Flower ” yang berada di Lorong Satria Kelurahan sungai buah yang merasakan penjualan tanaman hias menurun. Jika sebelumnya dapat diperoleh permintaan grosir tanaman hingga 8 kali dalam 1 minggu, kini menurun menjadi 1 kali atau bahkan tidak ada permintaan dalam 1 minggu. Untuk membantu memberikan alternatif penjualan, tim mengusulkan kegiatan pelatihan dan pendampingan digital marketing kepada pelaku UMKM usaha tanaman hias. Digital marketing dalam hal ini adalah dengan cara menandai lokasi bisnis dan memberi informasi bisnis di google map, memperluas pemasaran melalui instagram, dan membuat katalog di WhatsApp bisnis. Berbagai solusi pemasaran digital yang ditawarkan membantu penyebaran informasi produk dan jasa  para pelaku tanaman hias lebih luas sehingga penjualan  akan kembali meningkat. Melalui kegiatan ini diharapkan mampu meningkatkan pengetahuan para anggota komunitas petani tanaman hias tentang manfaat digital marketing yang dapat diakses dimana saja dan kapan saja. Sehingga, penjualan dan pendapatan para pelaku Usaha  tanaman hias diharapkan dapat semakin meningkat tidak hanya selama pandemi, namun juga di masa yang akan datang setelah pandemi berakhir
Pelatihan Penelitian Tindak Kelas Kelompok PPG Prajabatan Angkatan II Program Studi Pendidikan Jasmani, Kesehatan dan Olahraga di Kota Makassar
Authors: Sudirman Sudirman , Sahabuddin Sahabuddin , Palmizal Palmizal
Published: December 2022

Pengabdian kepada masyarakat ini di laksanakan dalam bentuk pelatihan Penelitian tindakan Kelas (Classroom Action Research) bagi Kelompok PPG Prajabatan Angkatan II Program Studi Pendidikan Jasmani, Kesehatan dan Olahraga di Kota Makassar. Kegiatan ini bertujuan untuk : memperbaiki dan atau meningkatkan kualitas praktek pembelajaran secara berkesinambungan sehingga meningkatan mutu hasil instruksional; mengembangkan keterampilan guru; meningkatkan relevansi; meningkatkan efisiensi pengelolaan instruksional serta menumbuhkan budaya meneliti pada komunitas guru-guru pendidikan jasmani. Manfaat Kegiatan ini diharapkan para bagi Kelompok PPG Prajabatan Angkatan II Program Studi Pendidikan Jasmani, Kesehatan dan Olahraga di Kota Makassar; antara lain: (1) PTK sangat kondusif untuk membuat guru menjadi peka tanggap terhadap dinamika pembelajaran di kelasnya. Dia menjadi reflektif dan kritis terhadap lakukan.apa yang dia dan muridnya (2) PTK dapat meningkatkan kinerja guru sehingga menjadi profesional. Guru tidak lagi sebagai seorang praktis, yang sudah merasa puas terhadap apa yang dikerjakan selama bertahun-tahun tanpa ada upaya perbaikan dan inovasi, namun juga sebagai peneniliti di bidangnya. (3) Dengan melaksanakan tahapan-tahapan dalam PTK, guru mampu memperbaiki proses pembelajaran melalui suatu kajian yang dalam terhadap apa yang terhadap apa yang terjadi di kelasnya. Tindakan yang dilakukan guru semata-mata didasarkan pada  masalah aktual dan faktual yang berkembang di kelasnya. (4) Pelaksanaan PTK tidak menggangu tugas pokok seorang guru karena dia tidak perlu meninggalkan kelasnya. PTK merupakan suatu kegiatan penelitian yang terintegrasi dengan pelaksanaan proses pembelajaran. (5) Dengan melaksanakan PTK guru menjadi kreatif karena selalu dituntut untuk melakukan upaya-upaya inovasi sebagai implementasi dan adaptasi berbagai teori dan teknik pembelajaran serta bahan ajar yang dipakainya. Guna memecahkan masalah memerlukan suatu kegiatan yang terprogram untuk pelatihan dan pengetahuan praktis kepada mereka dalam bentuk pelatihan khusus yang dapat dengan mudah mereka transfer dalam kemampuan. Metode yang diberikan berupa: (1) Metode Ceramah dan penyampaian makalah: terutama menyangkut penyampaian materi yang sifatnya teoretik. (2) Praktek yaitu aplikasi dari teoretik tentang penelitian tindakan kelas yang dituangkan dalam pembuatan proposal penelitian dilanjutkan dengan simulasi pelaksanaan penelitian tindakan kelas. (3) Metode diskusi dilaksanakan bila-hal-hal yang tidak dapat dipecahkan dan dirasa memerlukan penyelesaian.
Pelatihan Manajemen Pemasaran Dan Pengelolaan Permodalan Pada UMKM Usaha Pande Besi di Desa Tanjung Pinang Kecamatan Tanjung Batu Kabupaten Ogan Ilir
Authors: Roni Mukran , Terzaghi M Titan , Septayuda Irwan , Asmanita Asmanita , Jemakmun Jemakmun , Wahasusmiah Rolia , Afriansyah A
Published: December 2022

Kegiatan pengabdian ini dilakukan di Desa Tanjung Pinang Kecamatan Tanjung Batu Kabupaten Ogan Ilir pada usaha pandai besi. Tujuan dalam kegiatan ini adalah membekali peserta dengan ilmu manajemen pemasaran dan permodalan sehingga diharapkan dapat meningkatkan pengetahuan yang bersangkutan, memaksimalkan potensi usaha, dan meningkatkan kesejahteraan pelaku usaha. Metode pengabdian ini dilaksanakan dengan pemberian pelatihan melalui ceramah, diskusi, tanya-jawab, dan contoh. Hasil berupa peningkatan pemahaman mengenai manajemen pemasaran dan pengelolaan permodalan yang berdampak pada perbaikan kemampuan dalam strategy pemasaran dan permodalan yang akhirnya mendorong peningkatan kesejahteraan pelaku usaha.
On the issue of training and education of national oriented legal elite in national universities of Ukraine
Authors: Miroshnichenko M. I.
Published: December 2022

The article draws attention to one of the centers of university legal education in Ukraine, namely the place and importance of the history of Ukrainian law as a science and academic discipline in the university education of a lawyer in the post-genocidal, post-imperial period of reconstruction of Ukraine. digitalization. Separately, the emphasis is on the author’s vision of ways to improve the methodology of teaching the history of Ukrainian law for lawyers. It is emphasized that the transdisciplinary methodology, which was declared by UNESCO to be the leading one in the organization of the educational process in the XXI century, is a scientific guideline in favor of preserving the History of Ukrainian law with the status of a mandatory discipline in the educational process. The teaching of the History of Ukrainian Law should revolve around the idea of an organic combination of priority universal legal values created over the millennia of world history with national legal values. Thus, given the purpose of university higher education, a lawyer, if he wants to become a real specialist, should get a good basic legal education and constantly improve their knowledge in this area. In this case, without an in-depth study of the history of law and the state can not do. Deep penetration into the essence of legal phenomena in the learning process is possible only through the joint efforts of both theorists and historians of law. Historical and legal knowledge is important for solving current legal problems. The use of online tools only promotes more active communication between the student and the teacher. They complement rather than replace traditional lectures and seminars in classrooms. As a basis for developing the curriculum, it would be good to take the thesis that Ukrainian law is a social institution, with a pronounced spiritual and rational mechanism for regulating human behavior, meaningful and essentially a reflection of the existing socio-cultural reality in Ukraine. It is expedient to build a training course on the basis of problem or problem-chronological study of program material. The main emphasis in teaching should focus on research work, when the student is involved by the teacher in the field of their research and together they create space for new knowledge.

Key words: Ukraine, postimperial period, reconstruction, higher legal education, university legal education, history of Ukrainian law, methodology of history of Ukrainian law, methods of teaching discipline, information society, digitalization.
The concept of respect for human rights in conditions of increased risks
Authors: Riabchenko O. Р.
Published: December 2022

The article is devoted to highlighting the main problems of human rights in high risk and the formation of the concept of human rights in such conditions as a systematic approach to the formation and implementation of public policy in this area. The necessity of development and adoption of the package of necessary state programs is proved and the program of protection of the civilian population is allocated among them. The structure of the state program of protection of the civilian population is offered, which provides four blocks: legal protection; psychological protection; physical protection (basics of self-defense); fire training. It is noted that among all these blocks, psychological protection and fire training are of paramount importance in the current conditions of Russia’s armed aggression. The implementation of such a program includes trainings, preparation and dissemination of methodological literature, introduction of interactive methods, training of trainers, wide popularization in the media, as well as with the use of Internet resources. It is stated that the concept of respect for human rights provides a list of tasks, the solution of which is aimed at forming a theoretical and legal basis for the development of these specific state programs and the expected results.

Key words: human rights, concept, increased risk, condition, martial law, emergency situation.
Strategic directions of ensuring legal reform in Ukraine in the conditions of today’s challenges
Authors: Batanova N. М.
Published: December 2022

The article considers strategic directions of ensuring legal reform in the conditions of the Russian-Ukrainian war and post-war reconstruction of the state. Emphasis is placed on the need to make systemic changes to the legislation of Ukraine on human rights, local self-government, administrative and territorial organization, democracy, parliamentarism, justice, national security and defense. The scale of the changes that national legislation must undergo, their content and form, objectively require comprehensive legal reform in Ukraine.

The main strategic directions of ensuring the legal reform of Ukraine are highlighted: 1) improvement of the legislative support of the human rights system, the reality and effectiveness of guarantees for their implementation and protection; 2) strengthening the foundations of Ukrainian unitarism, protection of the sovereignty and territorial integrity of Ukraine; 3) strengthening the protection of Ukrainian statehood, legislative support of national security as the most important function of the state, the affairs of the entire Ukrainian people; 4) ensuring and guaranteeing the decisive role of the Ukrainian people in the constitution and functioning of all forms of public power; 5) increasing the role and place of the judiciary in ensuring the functioning of the state and civil society; 6) decentralization and functioning of real local self-government; 7) raising the level of awareness in the field of human rights, development of the system of legal education, including improving the system of legal education and science.

Key words: legal reform, strategy, Constitution, law, political system, legal system, rule of law
The role of legal reform in ensuring legal development in the war and postwar periods
Authors: Kryvytskyi Y. V.
Published: December 2022

Theoretical and methodological knowledge about the importance of legal reform in ensuring legal development in the war and postwar periods is generalized and expanded. It is substantiated that the relationship between legal development and legal reform is one of the important theoretical and practical problems of modern jurisprudence and legal life, without the solution of which it is difficult to properly navigate the prospects of legal change in the future.

Key words: development, legal development, reform, legal reform, war, war in Ukraine.
Extradition models within the framework of international cooperation of states in criminal cases
Authors: Hrytskiv A. V.
Published: December 2022

The article examines the basic models of extradition within the framework of international cooperation of states in criminal cases. It is emphasized that modern models of issuing persons in criminal cases that develop and expand within international organizations are distinguished. Adoption by international organizations of conventions as multilateral international treaties has a key role in the legal regulation of issues of issuance of persons and the formation of new models. The following models include: Universal Model Issuance within the UN, Model of the Institute of Issuance within the Council of Europe, Model of the Institute for Issuance under the European Union. The article describes the last model. It was noted that before the formation of the European Union in 1993, 28 Member States relied on the European Convention on the Issuance of Persons in 1957 and most European countries implemented the rules of this Convention. Therefore, some other international legal acts were adopted within the framework of this international organization. For example, the Council of Europe Framework on the European Arrest Order and the procedures for transferring offenders between Member States of 13 June 2002, which defines new directions of development of the Institute for the Issuance of Persons. Its feature is the accelerated process and the maximum possibility of issuing persons. It is this model that is now more in demand and new. It is concluded that each state retains its identity in the regulation of issues of issuing persons who assist in the formation of a national model of issuing persons in the framework of international cooperation in criminal cases. In no state in the world, you can find the full similarity of the norms for the issuance of persons.

Key words: extradition, international search, legal system, extradition models, European arrest warrant, European Union, international legal acts
«Separate opinion» as a unique independent genre of judicial discourse: practice of foreign courts
Authors: Kupianska A. M.
Published: December 2022

The article examines the main characteristics of a separate opinion of the judge as a unique independent genre of judicial discourse. It is emphasized that a special opinion of consent, or simply opinion, is declared in cases where the judge has no objection to the decision of the college itself and joins it, but either considers the above arguments or ways of resolving the legal dispute unsuccessful, or brings to argumentation additional consideration. This type of special opinion is characterized by greater freedom of expression of legal position in terms of content and arguments. It is noted that the constitutional justice of Ukraine formed a model of complete openness of a separate opinion of the judge of the Constitutional Court of Ukraine, the highest degree of which is the publication of a separate opinion together with the decision. A separate opinion of the judge of the Constitutional Court of Ukraine is determined by such that should not be confidential. It is proposed to identify a special opinion of a judge of a collegial body as an optional, structural and functional element of a judgment that is entered into the text of a decision or attached to it in the form of a separate document that has no obligatory legal force, but exists in an inseparable logical, semantic and structural. The main court decision, which determines its content and context and is characterized by individual argumentation, emotionality, imagery and evaluation. A separate opinion is an expression of a position on an issue that has already been decided by the court. This is the cry of the soul about the fact that certain arguments of the judge were not reflected in the motivational part of the court decision, or he does not agree with the decisive part of it. Special opinion is a unique independent genre of judicial discourse, in which argumentation is achieved, on the one hand, by logic and appeal to the letter of the law, and on the other, by the use of various means of emotional linguistic influence. The Institute of Distinctive Opinions serves as a guarantor of judicial independence and enables judges to position themselves not only as a part of the discursive expert community, but also as a sovereign person, a carrier and translator of a subjective creative legal position.

Key words: a separate opinion of the judge, judicial discourse, constitutional justice, the Constitutional Court of Ukraine, legal positions, court decisions, argumentation.
Contact correction of postkeratoplastic ametropia in patients after deep anterior lamellar keratoplasty
Authors: Sinitsyn M. V. , Pozdeeva N. A. , Bodrova S. G.
Published: December 2022

Purpose. To conduct a clinical and functional analysis of contact correction of postkeratoplastic ametropia with rigid gas-permeable scleral lenses (RGPSL) in patients after deep anterior lamellar keratoplasty.

Material and methods. Clinical and functional results of correction of postkeratoplastic ametropia with RGPSL were analyzed on the basis of a comprehensive examination of 35 eyes (35 patients) after deep anterior lamellar keratoplasty (DALK) performed for stage III keratoconus. The examination included patients who had low corrected visual acuity with spectacle correction and the corneal suture was removed 1 year or more after deep anterior lamellar keratoplasty. All patients underwent: autorefractokeratometry, visometry, biomicroscopy, computed keratotopography, aberrometry, endothelial cell density calculation, analysis of corneal graft biomechanical properties, optical coherence tomography of the corneal graft. The mean age of the patients was 29 ± 8 years. In all patients, RGPSL was selected on average 1.5 ± 0.85 years after DALK. All RGPSL were from OKVision (OKV-RGP Onefit Med, Canada). The follow-up period was 6 months.

Results. The uncorrected visual acuity was 1.0 ± 1.18 LogMAR, the best corrected visual acuity (BCVA) with glasses was 0.5 ± 0.8 LogMAR, the spherical refractive component was 2.44 ± 2.12 D, the cylindrical refractive component was -6,25 ± 1.24 D. The average value of BCVA in RGPSL increased to 0.72 ± 0.14 (p = 0.0054). The obtained result remained stable during 6 months of observation. In the analysis of corneal aberrations measured with the OPD-Scan II device before and after 6 months after wearing of RGPSL was noted a statistically significant decrease in total corneal aberrations, including higher-order ones. After 6 months of observation, according to Confoscan-4 data was noted the decrease in the density of corneal graft endothelial cells from 2526 ± 332 to 2510 ± 302 cells/mm² (p = 0.0326) and it corresponded to physiological losses.

Conclusions. Clinical and functional analysis of the correction of postkeratoplastic ametropia with rigid gas-permeable scleral lenses during 6 months of follow-up showed high optical eﬃciency and safety of their use during the entire observation period.
Pelatihan Pemasangan Lampu Otomatis pada Panti Asuhan Al-Husna Landungsari Kabupaten Malang
Authors: Waluyo Waluyo , Sarosa Moechammad , Yulianto Ahmad Wilda , Saptono Rachmad , Rakhmania Amalia Eka
Published: December 2022

Pengabdian kepada masyarakat ini ditujukan pada warga panti asuhan Al-Husna, untuk memberikan bekal keterampilan tentang dasar-dasar prinsip dasar rangkaian otomatisasi listrik. Dengan adanya pelatihan ini diharapkan peserta anak didik panti bisa lebih memahami dan mempunyai minat yang besar, khususnya tentang dunia elektronika dan aplikasi sensor otomasi. Kegiatan diawali dengan penjelasan singkat mengenai komponen elektronika yang digunakan dan cara kerjanya, eksperimen pada protoboard, kemudian pengujian lampu otomatis. Sensor passive infra red (PIR) digunakan pada pelatihan ini karena sifat kerjanya yang sederhana, sehingga mudah dipahami oleh peserta awam. Di akhir kegiatan, terlihat bahwa kemampuan dan pengetahuan peserta mengenai komponen elektronika meningkat. Peserta dengan tingkat pendidikan yang lebih tinggi, dalam hal ini SMA dapat menyerap informasi dan keterampilan yang lebih baik dibandingkan peserta dengan tingkat pendidikan SMP karena sudah memiliki dasar ilmu kelistrikan yang lebih baik.
Penyuluhan Deradikalisasi Pemahaman Ajaran Islam Di Desa Sitirejo Kecamatan Wagir Kabupaten Malang
Authors: Abdul Chalim  , Fadholi  , Sri Hudiarini  , Ahmad Baha'uddin  , Koesmarijanto  , Novitasari Ane fany
Published: December 2022

Dewasa ini paham radikalisme menjadi ancaman serius bagi kehidupan berbangsa dan beragama. Ruh Islam sebagai penebar rahmat bagi seluruh alam menjadi pudar karena dampak dari tindakan intoleran yang dialkukan oleh sebagian umat Islam. Pemahaman Islam yang radikal menjadikan praktik-praktik yang sebenarnya semakin jauh dari makna Islam yang hakiki. Misi Islam yang menyebarkan kedamaian rahmatan lil alamin berubah menjadi penebar terror. Setelah dilakukan penyuluhan Deradikalisasi Pemahaman Ajaran Agama Islam, Masyarakat menyadari bahwa pelu adanya filter dari berbagai informasi yang masuk baik informasi melalui ceramah-ceramah Agama secara langsung maupun ceramah Agama yang bisa diakses melalui media online seiring merebaknya organisasi[1]organisasi tertentu yang mengatasnamakan Islam dan menggunakan kekerasan dalam setiap gerakannya misalnya al-Qaeda, Jama’ah Islamiyah, Ikhwan al-Muslimin dan yang paling fenomenal saat ini adalah ISIS (Islamic State of Iraq and Syria), NII Negara Islam Indonesia
Determinan Kejadian Gastritis Pada Remaja: Studi Pada Mahasiswa Program Studi Kesehatan Masyrakat Universitas Nusa Cendana Kupang
Authors: Ratukore Ronny Syamuel J. P. , Manurung Imelda F. E. , Tira Deviarbi Sakke
Published: December 2022

Penyakit gastritis merupakan peradangan yang terjadi pada mukosa lambung.Gastritis merupakan salah satu penyakit yang umumnya diderita oleh kalangan remaja. Remaja seringkali meremehkan kondisi kesehatan mereka, hal ini disebabkan kehidupan yang mereka jalani dipenuhi dengan berbagai aktivitas sehingga mereka cenderung lupa untuk memperhatikan gaya hidup mereka. Tujuan penelitian ini yaitu untuk melihat hubungan antara status tempat tinggal, pola makan, kebiasaan konsumsi kopi, tingkat stres dan penggunaan OAINS dengan kejadian gastritis pada remaja. Penelitian ini merupakan penelitian kuantitatif dengan desain studi Cross-Sectional. Penelitian ini dilakukan di FKM Universitas Nusa Cendana Kupang dengan sampel sebanyak 130 mahasiswa. Tehnik pengambilan sampel yaitu Stratified Random Sampling. Analisis data yang digunakan adalah analisis univariat dan bivariat dengan uji statisti Chi-Square. Hasil penelitian menunjukan bahwa variabel status tempat tinggal (p =0,046) tinngal sendiri (57,7%) dan tinggal bersama orang tua/keluarga (42,3%), pola makan (p =0,003) buruk (47,7%) dan baik (52,3%), tingkat stres (p =0,000) tinggi (52,3%) dan rendah (47,7%), dan penggunaan OAINS (p =0,013) menggunakan (46,9%) dan tidak menggunakan (53,1%) memiliki hubungan dengan kejadian gastritis, sedangkan variabel kebiasaan konsumsi kopi (p=0,641) tinggi (52,3%) dan rendah (47,7%) tidak memiliki hubungan dengan kejadia gastritis. Kesimpulan dari penelitian ini adalah adalah status tempat tinggal, pola makan, tingkat stres, dan penggunaan OAINS merupakan determinan kejadian gastritis pada remaja. Saran peneliti adalah mahasiswa dapat meningkatkan pengetahuan tentang penyakit tidak menular salah satunya gastritis dan upaya pencegahannya
Deteksi Madu Palsu Dan Kualitas Madu Dengan Enzim Diastase
Authors: Ichsan Dewi Susetiyany , Hafidzah Talitha Syifa , Putri Septihany Berliana , Aurene Shizen Valere , Nurdin Isnawati
Published: December 2022

Permintaan madu yang meningkat yang tidak diimbangi dengan produksi madu yang memadai, meningkatkan risiko pemalsuan madu. Keaslian dan kualitas madu dapat ditentukan menggunakan metode konvensional (uji larut, uji keruh, uji buih, uji pemanasan) dan kimia. Uji kimia enzim diastase dapat digunakan sebagai indikator dalam penilaian kemurnian dan kualitas madu karena enzim tersebut diproduksi langsung oleh lebah. Penelitian ini bertujuan untuk menganalisis pemanfaatan uji enzim diastase dalam mendeteksi madu palsu dan kualitas madu di Kota Palu. Metode yang digunakan adalah uji enzim diastase sesuai SNI 8664: 2018 menggunakan spektrofotometri UV-Vis. Sampel berupa 9 sampel registrasi MD, 3 registrasi ML, 9 registrasi PIRT dan 1 madu palsu. Pengujian dan pengambilan data dilakukan di Balai POM Palu. Berdasarkan hasil pengujian, keaslian dan kualitas madu tidak dapat ditentukan hanya dengan metode konvensional karena pengamatannya tidak terukur secara pasti. Pada uji kimia enzim diastase didapatkan 17 sampel dari 22 sampel mempunyai kualitas baik karena memenuhi syarat SNI. Nilai Diastase Number (DN) yang diperoleh dari 0.64 sampai 23.11 sedangkan madu palsu nilai DN = 0 (tidak terdeteksi). Tidak terdeteksinya enzim diastase dalam contoh madu dapat diindikasikan sebagai madu palsu karena dalam contoh madu tersebut hanya mengandung gula dan tidak mengandung enzim diastase
CRISPR/Cas tools for enhancing the biopreservation ability of lactic acid bacteria in aquatic products
Authors: Dong Huina , Wang Huiying , Fu Shaoping , Zhang Dawei
Published: December 2022

Lactic acid bacteria (LAB) plays a crucial role in aquatic products biopreservation as it can inhibit many bacteria, in particular the specific spoilage organisms (SSOs) of aquatic products, by competing for nutrients or producing one or more metabolites which have antimicrobial activity, such as bacteriocins. Lactobacillus spp. and Lactococcus spp. are the most commonly used Lactic acid bacterias in aquatic products preservation. The improvement of gene editing tools is particularly important for developing new lactic acid bacteria strains with superior properties for aquatic products biopreservation. This review summarized the research progress of the most widely used CRISPR/Cas-based genome editing tools in Lactobacillus spp. and Lactococcus spp. The genome editing tools based on homologous recombination and base editor were described. Then, the research status of CRISPRi in transcriptional regulation was reviewed briefly. This review may provide a reference for the application of CRISPR/Cas-based genome editing tools to other lactic acid bacteria species.
Aptamer Nanomaterials for Ovarian Cancer Target Theranostics
Authors: Zhao Jing , Tan Wenxi , Zheng Jingying , Su Yuanzhen , Cui Manhua
Published: December 2022

Ovarian cancer is among the leading causes of gynecological cancer-related mortality worldwide. Early and accurate diagnosis and an effective treatment strategy are the two primary means of improving the prognosis of patients with ovarian cancer. The development of targeted nanomaterials provides a potentially efficient strategy for ovarian cancer theranostics. Aptamer nanomaterials have emerged as promising nanoplatforms for accurate ovarian cancer diagnosis by recognizing relevant biomarkers in the serum and/or on the surface of tumor cells, as well as for effective ovarian cancer inhibition via target protein blockade on tumor cells and targeted delivery of various therapeutic agents. In this review, we summarize recent advances in aptamer nanomaterials as targeted theranostic platforms for ovarian cancer and discusses the challenges and opportunities for their clinical application. The information presented in this review represents a valuable reference for creation of a new generation of aptamer nanomaterials for use in the precise detection and treatment of ovarian cancer.
An evaluation of KIF20A as a prognostic factor and therapeutic target for lung adenocarcinoma using integrated bioinformatics analysis
Authors: Sun Dongjie , Zhang Haiying , Zhang Chi , Wang Lina
Published: December 2022

The identification of prognostic and therapeutic biomarkers is essential to reduce morbidity and mortality from lung adenocarcinoma (LUAD). This study aimed to identify a reliable prognostic and therapeutic biomarker for LUAD using integrated bioinformatics. Based on the cancer genome atlas (TCGA) and genome-tissue expression (GTEx) analyses, KIF20A has been identified as the hub gene. Following validation using a series of cohorts, survival analysis, meta-analysis, and univariate Cox analysis was conducted. ESTIMATE and CIBERSORT algorithms were then used to study the association of KIF20A with the tumor microenvironment (TME) and the percentage of tumor-infiltrating immune cells (TICs). In vitro experiments were conducted to determine the function of KIF20A. Finally, there was a negative association between the expression of the KIF20A and overall survival, progression-free survival, and disease-free survival, which was confirmed by meta-analysis and COX analysis. Furthermore, KIF20A also had a potential role of altering the TME and TICs proportions in LUAD. Validations in vitro were performed on A549 and PC-9 cell lines, and we found that the knockdown of KIF20A exhibited inhibitory effects on cell proliferation, resulted in cell cycle arrest during the G2/M phase, and induced cellular apoptosis. Our study demonstrated that KIF20A could be utilized as a reliable prognostic marker and treatment target for LUAD. However, further studies are required to validate these findings.
Genome-wide transposon mutagenesis analysis of Burkholderia pseudomallei reveals essential genes for in vitro and in vivo survival
Authors: Wong Yee-Chin , Naeem Raeece , Abd El Ghany Moataz , Hoh Chee-Choong , Pain Arnab , Nathan Sheila
Published: December 2022

Introduction

Burkholderia pseudomallei, a soil-dwelling microbe that infects humans and animals is the cause of the fatal disease melioidosis. The molecular mechanisms that underlie B. pseudomallei’s versatility to survive within a broad range of environments are still not well defined.

Methods

We used the genome-wide screening tool TraDIS (Transposon Directed Insertion-site Sequencing) to identify B. pseudomallei essential genes. Transposon-flanking regions were sequenced and gene essentiality was assessed based on the frequency of transposon insertions within each gene. Transposon mutants were grown in LB and M9 minimal medium to determine conditionally essential genes required for growth under laboratory conditions. The Caenorhabditis elegans infection model was used to assess genes associated with in vivo B. pseudomallei survival. Transposon mutants were fed to the worms, recovered from worm intestines, and sequenced. Two selected mutants were constructed and evaluated for the bacteria’s ability to survive and proliferate in the nematode intestinal lumen.

Results

Approximately 500,000 transposon-insertion mutants of B. pseudomallei strain R15 were generated. A total of 848,811 unique transposon insertion sites were identified in the B. pseudomallei R15 genome and 492 genes carrying low insertion frequencies were predicted to be essential. A total of 96 genes specifically required to support growth under nutrient-depleted conditions were identified. Genes most likely to be involved in B. pseudomallei survival and adaptation in the C. elegans intestinal lumen, were identified. When compared to wild type B. pseudomallei, a Tn5 mutant of bpsl2988 exhibited reduced survival in the worm intestine, was attenuated in C. elegans killing and showed decreased colonization in the organs of infected mice.

Discussion

The B. pseudomallei conditional essential proteins should provide further insights into the bacteria’s niche adaptation, pathogenesis, and virulence.
Proteome analysis of Ehrlichia chaffeensis containing phagosome membranes revealed the presence of numerous bacterial and host proteins
Authors: Kondethimmanahalli Chandramouli , Ganta Roman R.
Published: December 2022

Tick-transmitted Ehrlichia chaffeensis, the causative agent for human monocytic ehrlichiosis, resides and multiplies within a host cell phagosome. Infection progression of E. chaffeensis includes internalization into a host cell by host cell membrane fusion events following engulfment leading to the formation of E. chaffeensis containing vacuole (ECV). Revealing the molecular composition of ECV is important in understanding the host cellular processes, evasion of host defense pathways and in defining host-pathogen interactions. ECVs purified from infected host cells were analyzed to define both host and bacterial proteomes associated with the phagosome membranes. About 160 bacterial proteins and 2,683 host proteins were identified in the ECV membranes. The host proteins included predominantly known phagosome proteins involved in phagocytic trafficking, fusion of vesicles, protein transport, Ras signaling pathway and pathogenic infection. Many highly expressed proteins were similar to the previously documented proteins of phagosome vacuole membranes containing other obligate pathogenic bacteria. The finding of many bacterial membrane proteins is novel; they included multiple outer membrane proteins, such as the p28-Omps, the 120 kDa protein, preprotein translocases, lipoproteins, metal binding proteins, and chaperonins, although the presence of ankyrin repeat proteins, several Type I and IV secretion system proteins is anticipated. This study demonstrates that ECV membrane is extensively modified by the pathogen. This study represents the first and the most comprehensive description of ECV membrane proteome. The identity of many host and Ehrlichia proteins in the ECV membrane will be a valuable to define pathogenic mechanisms critical for the replication of the pathogen within macrophages.
Phenotype and function of peripheral blood γδ T cells in HIV infection with tuberculosis
Authors: Zou Shi , Xiang Yanni , Guo Wei , Zhu Qi , Wu Songjie , Tan Yuting , Yan Yajun , Shen Ling , Feng Yong , Liang Ke
Published: December 2022

Background

Although γδ T cells play an essential role in immunity againstHuman Immunodeficiency Virus(HIV) orMycobacterium tuberculosis(MTB), they are poorly described in HIV infection with tuberculosis (TB).

Methods

The phenotypic and functional properties of peripheral blood γδ T cells in patients with HIV/TB co-infection were analyzed compared to healthy controls and patients with HIV mono-infection or TB by direct intracellular cytokine staining (ICS).

Results

The percentage of Vδ1subset in HIV/TB group was significantly higher than that in TB group, while the decreased frequency of the Vδ2and Vγ2Vδ2subsets were observed in HIV/TB group than in TB group. The percentage of CD4+CD8-Vδ2subset in HIV/TB group was markedly lower than in TB group. However, the percentage of CD4+CD8+Vδ2subset in HIV/TB group was markedly higher than HIV group or TB group. A lower percentage TNF-α and a higher percentage of IL-17A of Vδ2subset were observed in HIV/TB group than that in HIV mono-infection. The percentage of perforin-producing Vδ2subset was significantly lower in HIV/TB group than that in HIV group and TB group.

Conclusions

Our data suggested that HIV/TB co-infection altered the balance of γδ T cell subsets. The influence of HIV/TB co-infection on the function of γδ T cells to produce cytokines was complicated, which will shed light on further investigations on the mechanisms of the immune response against HIV and/or MTB infection.
Biochemical and hematological factors associated with COVID-19 severity among Gabonese patients: A retrospective cohort study
Authors: N’dilimabaka Nadine , Mounguegui Dieudonné Mounguegui , Lekana-Douki Sonia Etenna , Yattara Marisca Kandet , Obame-Nkoghe Judicaël , Longo-Pendy Neil Michel , Koumba Ingrid Precilya Koumba , Mve-Ella Octavie Lauris Banga , Moukouama Schedy Koumba , Dzembo Cresh Emelya , Bolo Lauriane Yacka , Biyie-Bi-Ngoghe Prudence , Mangouka Guignali Laurette , Nzenze Jean-Raymond , Lekana-Douki Jean-Bernard
Published: December 2022

The COVID-19 disease presents a large range of clinical manifestations and includes asymptomatic, mild, and severe cases. The level of severity is related to parameters associated with immunity, genetics, and biochemistry. Africa shows one of the lowest COVID-19 fatality rates but very few data on the biochemical markers of COVID-19 in patients and the factors associated with disease severity are available for the continent. In Gabon, the COVID-19 fatality rate is only 0.63% but almost no data on biomarkers in COVID-19 patients have been published. Both the number of COVID-19 cases and the mortality rate reported in Africa in general, and in Gabon in particular, are lower than in non-African countries. As such, understanding the factors associated with disease severity in Gabonese patients is a crucial step to better understand the disease in the African context and prepare for future COVID-19 waves and other epidemics of emerging diseases. Here, we compared biochemical and hematological markers among 753 Gabonese COVID-19 patients with asymptomatic (184/753), mild/moderate (420/753), and severe/critical (149/753) forms of the disease using an Analysis of Variance (ANOVA) or a Kruskal-Wallis (KW) test. We modeled these parameters together with comorbidities, age, and sex to predict factors associated with disease severity by using a "binomial generalized linear model" utilizing the "package" stats of R software version 4.0.2. Our results showed that almost all the biochemical and hematological parameters (except creatinine, phosphorus, D-dimers, platelets, and monocytes) varied according to disease severity. However, age and the dysfunction of organs like the kidney, liver, and lung together with the decrease of electrolytes (chloride, potassium, and sodium) are the best predictors of disease severity in Gabonese patients.
5mC modification patterns provide novel direction for early acute myocardial infarction detection and personalized therapy
Authors: Guo Yiqun , Jiang Hua , Wang Jinlong , Li Ping , Zeng Xiaoquan , Zhang Tao , Feng Jianyi , Nie Ruqiong , Liu Yulong , Dong Xiaobian , Hu Qingsong
Published: December 2022

Background

Most deaths from coronary artery disease (CAD) are due to acute myocardial infarction (AMI). There is an urgent need for early AMI detection, particularly in patients with stable CAD. 5-methylcytosine (5mC) regulatory genes have been demonstrated to involve in the progression and prognosis of cardiovascular diseases, while little research examined 5mC regulators in CAD to AMI progression.

Method

Two datasets (GSE59867 and GSE62646) were downloaded from Gene Expression Omnibus (GEO) database, and 21 m5C regulators were extracted from previous literature. Dysregulated 5mC regulators were screened out by “limma.” The least absolute shrinkage and selection operator (LASSO) and support vector machine recursive feature elimination (SVM-RFE) algorithm were employed to identify hub 5mC regulators in CAD to AMI progression, and 43 clinical samples (Quantitative real-time PCR) were performed for expression validation. Then a logistic model was built to construct 5mC regulator signatures, and a series of bioinformatics algorithms were performed for model validation. Besides, 5mC-associated molecular clusters were studied via unsupervised clustering analysis, and correlation analysis between immunocyte and 5mC regulators in each cluster was conducted.

Results

Nine hub 5mC regulators were identified. A robust model was constructed, and its prominent classification accuracy was verified via ROC curve analysis (area under the curve [AUC] = 0.936 in the training cohort and AUC = 0.888 in the external validation cohort). Besides, the clinical effect of the model was validated by decision curve analysis. Then, 5mC modification clusters in AMI patients were identified, along with the immunocyte infiltration levels of each cluster. The correlation analysis found the strongest correlations were TET3—Mast cell in cluster-1 and TET3-MDSC in cluster-2.

Conclusion

Nine hub 5mC regulators (DNMT3B, MBD3, UHRF1, UHRF2, NTHL1, SMUG1, ZBTB33, TET1, and TET3) formed a diagnostic model, and concomitant results unraveled the critical impact of 5mC regulators, providing interesting epigenetics findings in AMI population vs. stable CAD.
Influence of placental exosomes from early onset preeclampsia women umbilical cord plasma on human umbilical vein endothelial cells
Authors: Gu Mengqi , Zhang Fengyuan , Jiang Xiaotong , Chen Pengzheng , Wan Shuting , Lv Qingfeng , Lu Yuan , Zhou Qian , Wang Yanyun , Li Lei
Published: December 2022

Background

Early onset preeclampsia (EOSP, PE) is characterized by hypertension, proteinuria, and endothelial dysfunction. Oxidative stress-induced trophoblast dysfunction is a major pathology in PE. Placental exosomes are extracellular vesicles that are involved in “mother-placenta-foetal communication” and can regulate the biological functions of endothelial cells. Our study was designed to evaluate placental exosomes effects on endothelial cells.

Methods

Umbilical cord blood from normal pregnant women and patients with PE were collected. A hypoxia/reoxygenation (H/R) model in human first trimester extravillous trophoblast cell (HTR8/SVneo) line to simulate the PE model of oxidative stress in vitro. Then, placental exosomes (i.e., NO-exo, H/R-exo, N-exo, and PE-exo) were extracted and identified. Finally, the effects of placental exosomes on the biological functions of human umbilical vein endothelial cells (HUVECs) were further evaluated by performing a series of experiments.

Results

Placental exosomes had a double-membrane cup structure with diameters of 30–150 nm, and there was no obvious difference in placental exosomes. Compared with NO-exo and N-exo, H/R-exo and PE-exo inhibited HUVECs proliferation, tube formation and migration, increased permeability and apoptosis in vitro.

Conclusion

We hypothesize that H/R-exo and PE-exo impair vessel development by disrupted biological functions in endothelial cells, which may result in vascular disorders in offspring.