bluuebunny/crossref_metadata_2025 huggingface dataset:
On the Use of Correlation and MI as a Measure of Metabolite—Metabolite Association for Network Differential Connectivity Analysis
Authors: Jahagirdar Sanjeevan , Saccenti Edoardo
Published: April 2020

Metabolite differential connectivity analysis has been successful in investigating potential molecular mechanisms underlying different conditions in biological systems. Correlation and Mutual Information (MI) are two of the most common measures to quantify association and for building metabolite—metabolite association networks and to calculate differential connectivity. In this study, we investigated the performance of correlation and MI to identify significantly differentially connected metabolites. These association measures were compared on (i) 23 publicly available metabolomic data sets and 7 data sets from other fields, (ii) simulated data with known correlation structures, and (iii) data generated using a dynamic metabolic model to simulate real-life observed metabolite concentration profiles. In all cases, we found more differentially connected metabolites when using correlation indices as a measure for association than MI. We also observed that different MI estimation algorithms resulted in difference in performance when applied to data generated using a dynamic model. We concluded that there is no significant benefit in using MI as a replacement for standard Pearson’s or Spearman’s correlation when the application is to quantify and detect differentially connected metabolites.
Size Sorting of Exosomes by Tuning the Thicknesses of the Electric Double Layers on a Micro-Nanofluidic Device
Authors: Fujiwara Satoko , Morikawa Kyojiro , Endo Tatsuro , Hisamoto Hideaki , Sueyoshi Kenji
Published: April 2020

Exosomes, a type of extracellular vesicle with a diameter of 30–150 nm, perform key biological functions such as intercellular communication. Recently, size sorting of exosomes has received increasing attention in order to clarify the correlation between their size and components. However, such sorting remains extremely difficult. Here, we propose to sort their size by controlling their electrokinetic migration in nanochannels in a micro-nanofluidic device, which is achieved by tuning the thickness of the electric double layers in the nanochannels. This approach was demonstrated experimentally for exosomes smaller than 250 nm. Using different running buffer concentrations (1 × 10−3, 1 × 10−4, and 1 × 10−5 M), most of the exosomes larger than 140, 110, and 80 nm were successfully cut off at the downstream of the nanochannels, respectively. Therefore, it is clarified that the proposed method is applicable for the size sorting of exosomes.
Elucidating the Effect of Accelerated Carbonation on Porosity and Mechanical Properties of Hydrated Portland Cement Paste Using X-Ray Tomography and Advanced Micromechanical Testing
Authors: Zhang Hongzhi , Romero Rodriguez Claudia , Dong Hua , Gan Yidong , Schlangen Erik , Šavija Branko
Published: April 2020

Carbonation of hydrated cement paste (HCP) causes numerous chemo–mechanical changes in the microstructure, e.g., porosity, strength, elastic modulus, and permeability, which have a significant influence on the durability of concrete structures. Due to its complexity, much is still not understood about the process of carbonation of HCP. The current study aims to reveal the changes in porosity and micromechanical properties caused by carbonation using micro-beam specimens with a cross-section of 500 μm × 500 μm. X-ray computed tomography and micro-beam bending tests were performed on both noncarbonated and carbonated HCP micro-beams for porosity characterization and micromechanical property measurements, respectively. The experimental results show that the carbonation decreases the total porosity and increases micromechanical properties of the HCP micro-beams under the accelerated carbonation. The correlation study revealed that both the flexural strength and elastic modulus increase linearly with decreasing porosity.
Mineralogy and Fluid Regime of Formation of the REE-Late-Stage Hydrothermal Mineralization of Petyayan-Vara Carbonatites (Vuoriyarvi, Kola Region, NW Russia)
Authors: Prokopyev Ilya , Kozlov Evgeniy , Fomina Ekaterina , Doroshkevich Anna , Dyomkin Maxim
Published: April 2020

The Vuoriyarvi Devonian alkaline–ultramafic complex (northwest Russia) contains magnesiocarbonatites with rare earth mineralization localized in the Petyayan-Vara area. High concentrations of rare earth elements are found in two types of these rocks: (a) ancylite-dominant magnesiocarbonatites with ancylite–baryte–strontianite–calcite–quartz (±late Ca–Fe–Mg carbonates) ore assemblage, i.e., “ancylite ores”; (b) breccias of magnesiocarbonatites with a quartz–bastnäsite matrix (±late Ca–Fe–Mg carbonates), i.e., “bastnäsite ores.” We studied fluid inclusions in quartz and late-stage Ca–Fe–Mg carbonates from these ore assemblages. Fluid inclusion data show that ore-related mineralization was formed in several stages. We propose the following TX evolution scheme for ore-related processes: (1) the formation of ancylite ores began under the influence of highly concentrated (>50 wt.%) sulphate fluids (with thenardite and anhydrite predominant in the daughter phases of inclusions) at a temperature above300–350 °C; (2) the completion of the formation of ancylite ores and their auto-metasomatic alteration occurred under the influence of concentrated (40–45 wt.%) carbonate fluids (shortite and synchysite–Ce in fluid inclusions) at a temperature above 250–275 °C; (3) bastnäsite ores deposited from low-concentrated (20–30 wt.%) hydrocarbonate–chloride fluids (halite, nahcolite, and/or gaylussite in fluid inclusions) at a temperature of 190–250 °C or higher. Later hydrothermal mineralization was related to the low-concentration hydrocarbonate–chloride fluids (<15 wt.% NaCl-equ.) at 150–200 °C. The presented data show the specific features of the mineral and fluid evolution of ore-related late-stage hydrothermal rare earth element (REE) mineralization of the Vuoriyarvi alkaline–ultramafic complex.
rAAV-Mediated Overexpression of SOX9 and TGF-β via Carbon Dot-Guided Vector Delivery Enhances the Biological Activities in Human Bone Marrow-Derived Mesenchymal Stromal Cells
Authors: Meng Weikun , Rey-Rico Ana , Claudel Mickaël , Schmitt Gertrud , Speicher-Mentges Susanne , Pons Françoise , Lebeau Luc , Venkatesan Jagadeesh K. , Cucchiarini Magali
Published: April 2020

Scaffold-assisted gene therapy is a highly promising tool to treat articular cartilage lesions upon direct delivery of chondrogenic candidate sequences. The goal of this study was to examine the feasibility and benefits of providing highly chondroreparative agents, the cartilage-specific sex-determining region Y-type high-mobility group 9 (SOX9) transcription factor or the transforming growth factor beta (TGF-β), to human bone marrow-derived mesenchymal stromal cells (hMSCs) via clinically adapted, independent recombinant adeno-associated virus (rAAV) vectors formulated with carbon dots (CDs), a novel class of carbon-dominated nanomaterials. Effective complexation and release of a reporter rAAV-lacZ vector was achieved using four different CDs elaborated from 1-citric acid and pentaethylenehexamine (CD-1); 2-citric acid, poly(ethylene glycol) monomethyl ether (MW 550 Da), and N,N-dimethylethylenediamine (CD-2); 3-citric acid, branched poly(ethylenimine) (MW 600 Da), and poly(ethylene glycol) monomethyl ether (MW 2 kDa) (CD-3); and 4-citric acid and branched poly(ethylenimine) (MW 600 Da) (CD-4), allowing for the genetic modification of hMSCs. Among the nanoparticles, CD-2 showed an optimal ability for rAAV delivery (up to 2.2-fold increase in lacZ expression relative to free vector treatment with 100% cell viability for at least 10 days, the longest time point examined). Administration of therapeutic (SOX9, TGF-β) rAAV vectors in hMSCs via CD-2 led to the effective overexpression of each independent transgene, promoting enhanced cell proliferation (TGF-β) and cartilage matrix deposition (glycosaminoglycans, type-II collagen) for at least 21 days relative to control treatments (CD-2 lacking rAAV or associated to rAAV-lacZ), while advantageously restricting undesirable type-I and -X collagen deposition. These results reveal the potential of CD-guided rAAV gene administration in hMSCs as safe, non-invasive systems for translational strategies to enhance cartilage repair.
Photoluminescence of ZnO Nanowires: A Review
Authors: Galdámez-Martinez Andres , Santana Guillermo , Güell Frank , Martínez-Alanis Paulina R. , Dutt Ateet
Published: April 2020

One-dimensional ZnO nanostructures (nanowires/nanorods) are attractive materials for applications such as gas sensors, biosensors, solar cells, and photocatalysts. This is due to the relatively easy production process of these kinds of nanostructures with excellent charge carrier transport properties and high crystalline quality. In this work, we review the photoluminescence (PL) properties of single and collective ZnO nanowires and nanorods. As different growth techniques were obtained for the presented samples, a brief review of two popular growth methods, vapor-liquid-solid (VLS) and hydrothermal, is shown. Then, a discussion of the emission process and characteristics of the near-band edge excitonic emission (NBE) and deep-level emission (DLE) bands is presented. Their respective contribution to the total emission of the nanostructure is discussed using the spatial information distribution obtained by scanning transmission electron microscopy−cathodoluminescence (STEM-CL) measurements. Also, the influence of surface effects on the photoluminescence of ZnO nanowires, as well as the temperature dependence, is briefly discussed for both ultraviolet and visible emissions. Finally, we present a discussion of the size reduction effects of the two main photoluminescent bands of ZnO. For a wide emission (near ultra-violet and visible), which has sometimes been attributed to different origins, we present a summary of the different native point defects or trap centers in ZnO as a cause for the different deep-level emission bands.
Ag2S QDs/Si Heterostructure-Based Ultrasensitive SWIR Range Detector
Authors: Tretyakov Ivan , Svyatodukh Sergey , Perepelitsa Aleksey , Ryabchun Sergey , Kaurova Natalya , Shurakov Alexander , Smirnov Mikhail , Ovchinnikov Oleg , Goltsman Gregory
Published: April 2020

In the 20th century, microelectronics was revolutionized by silicon—its semiconducting properties finally made it possible to reduce the size of electronic components to a few nanometers. The ability to control the semiconducting properties of Si on the nanometer scale promises a breakthrough in the development of Si-based technologies. In this paper, we present the results of our experimental studies of the photovoltaic effect in Ag2S QD/Si heterostructures in the short-wave infrared range. At room temperature, the Ag2S/Si heterostructures offer a noise-equivalent power of 1.1 × 10−10 W/√Hz. The spectral analysis of the photoresponse of the Ag2S/Si heterostructures has made it possible to identify two main mechanisms behind it: the absorption of IR radiation by defects in the crystalline structure of the Ag2S QDs or by quantum QD-induced surface states in Si. This study has demonstrated an effective and low-cost way to create a sensitive room temperature SWIR photodetector which would be compatible with the Si complementary metal oxide semiconductor technology.
Association of the SH2B1 rs7359397 Gene Polymorphism with Steatosis Severity in Subjects with Obesity and Non-Alcoholic Fatty Liver Disease
Authors: Perez-Diaz-del-Campo Nuria , Abete Itziar , Cantero Irene , Marin-Alejandre Bertha Araceli , Monreal J. Ignacio , Elorz Mariana , Herrero José Ignacio , Benito-Boillos Alberto , Riezu-Boj Jose I. , Milagro Fermín I. , Tur Josep A. , Martinez J. Alfredo , Zulet M. Angeles
Published: April 2020

Non-alcoholic fatty liver disease (NAFLD) is a major cause of liver disease worldwide. Some genetic variants might be involved in the progression of this disease. The study hypothesized that individuals with the rs7359397 T allele have a higher risk of developing severe stages of NAFLD compared with non-carriers where dietary intake according to genotypes could have a key role on the pathogenesis of the disease. SH2B1 genetic variant was genotyped in 110 overweight/obese subjects with NAFLD. Imaging techniques, lipidomic analysis and blood liver biomarkers were performed. Body composition, general biochemical and dietary variables were also determined. The SH2B1 risk genotype was associated with higher HOMA-IR p = 0.001; and Fatty Liver Index (FLI) p = 0.032. Higher protein consumption (p = 0.028), less mono-unsaturated fatty acid and fiber intake (p = 0.045 and p = 0.049, respectively), was also referred to in risk allele genotype. Lipidomic analysis showed that T allele carriers presented a higher frequency of non-alcoholic steatohepatitis (NASH) (69.1% vs. 44.4%; p = 0.006). In the genotype risk group, adjusted logistic regression models indicated a higher risk of developing an advanced stage of NAFLD measured by FLI (OR 2.91) and ultrasonography (OR 4.15). Multinomial logistic regression models showed that risk allele carriers had higher liver fat accumulation risk (RRR 3.93) and an increased risk of NASH (RRR 7.88). Consequently, subjects carrying the T allele were associated with a higher risk of developing a severe stage of NAFLD. These results support the importance of considering genetic predisposition in combination with a healthy dietary pattern in the personalized evaluation and management of NAFLD.
Acamprosate Is a Substrate of the Human Organic Anion Transporter (OAT) 1 without OAT3 Inhibitory Properties: Implications for Renal Acamprosate Secretion and Drug–Drug Interactions
Authors: Antonescu Irina E. , Karlgren Maria , Pedersen Maria L. , Simoff Ivailo , Bergström Christel A. S. , Neuhoff Sibylle , Artursson Per , Steffansen Bente , Nielsen Carsten Uhd
Published: April 2020

Acamprosate is an anionic drug substance widely used in treating symptoms of alcohol withdrawal. It was recently shown that oral acamprosate absorption is likely due to paracellular transport. In contrast, little is known about the eliminating mechanism clearing acamprosate from the blood in the kidneys, despite the fact that studies have shown renal secretion of acamprosate. The hypothesis of the present study was therefore that renal organic anion transporters (OATs) facilitate the renal excretion of acamprosate in humans. The aim of the present study was to establish and apply OAT1 (gene product of SLC22A6) and OAT3 (gene product of SLC22A8) expressing cell lines to investigate whether acamprosate is a substrate or inhibitor of OAT1 and/or OAT3. The studies were performed in HEK293-Flp-In cells stably transfected with SLC22A6 or SLC22A8. Protein and functional data showed that the established cell lines are useful for studying OAT1- and OAT3-mediated transport in bi-laboratory studies. Acamprosate inhibited OAT1-mediated p-aminohippuric acid (PAH) uptake but did not inhibit substrate uptake via OAT3 expressing cells, neither when applied concomitantly nor after a 3 h preincubation with acamprosate. The uptake of PAH via OAT1 was inhibited in a competitive manner by acamprosate and cellular uptake studies showed that acamprosate is a substrate for OAT1 with a Km-value of approximately 700 µM. Probenecid inhibited OAT1-mediated acamprosate uptake with a Ki-value of approximately 13 µM, which may translate into an estimated clinically significant DDI index. In conclusion, acamprosate was identified as a substrate of OAT1 but not OAT3.
Protective Efficacy of Inhaled BCG Vaccination Against Ultra-Low Dose Aerosol M. tuberculosis Challenge in Rhesus Macaques
Authors: White Andrew D. , Sarfas Charlotte , Sibley Laura S. , Gullick Jennie , Clark Simon , Rayner Emma , Gleeson Fergus , Català Martí , Nogueira Isabel , Cardona Pere-Joan , Vilaplana Cristina , Dennis Mike J. , Williams Ann , Sharpe Sally A.
Published: April 2020

Ten million cases of tuberculosis (TB) were reported in 2018 with a further 1.5 million deaths attributed to the disease. Improved vaccination strategies are urgently required to tackle the ongoing global TB epidemic. In the absence of a validated correlate of protection, highly characterised pre-clinical models are required to assess the protective efficacy of new vaccination strategies. In this study, we demonstrate the application of a rhesus macaque ultra-low dose (ULD) aerosol M. tuberculosis challenge model for the evaluation of TB vaccination strategies by directly comparing the immunogenicity and efficacy of intradermal (ID) and aerosol BCG vaccination delivered using a portable vibrating mesh nebulizer (VMN). Aerosol- and ID-delivered Bacille Calmette-Guérin (BCG) induced comparable frequencies of IFN-γ spot forming units (SFU) measured in peripheral blood mononuclear cells (PBMCs) by ELISpot, although the induction of IFN-γ SFU was significantly delayed following aerosol immunisation. This delayed response was also apparent in an array of secreted pro-inflammatory and chemokine markers, as well as in the frequency of antigen-specific cytokine producing CD4 and CD8 T-cells measured by multi-parameter flow cytometry. Interrogation of antigen-specific memory T-cell phenotypes revealed that vaccination-induced CD4 and CD8 T-cell populations primarily occupied the central memory (TCM) and transitional effector memory (TransEM) phenotype, and that the frequency of CD8 TCM and TransEM populations was significantly higher in aerosol BCG-vaccinated animals in the week prior to M. tuberculosis infection. The total and lung pathology measured following M. tuberculosis challenge was significantly lower in vaccinated animals relative to the unvaccinated control group and pathology measured in extra-pulmonary tissues was significantly reduced in aerosol BCG-vaccinated animals, relative to the ID-immunised group. Similarly, significantly fewer viable M. tuberculosis CFU were recovered from the extra-pulmonary tissues of aerosol BCG-vaccinated macaques relative to unvaccinated animals. In this study, a rhesus macaque ULD M. tuberculosis aerosol challenge model was applied as a refined and sensitive system for the evaluation of TB vaccine efficacy and to confirm that aerosol BCG vaccination delivered by portable VMN can confer a significant level of protection that is equivalent, and by some measures superior, to intradermal BCG vaccination.
Design and Characterization of Phosphatidylcholine-Based Solid Dispersions of Aprepitant for Enhanced Solubility and Dissolution
Authors: Yeo Sooho , An Jieun , Park Changhee , Kim Dohyun , Lee Jaehwi
Published: April 2020

This study aimed to improve the solubility and dissolution of aprepitant, a drug with poor aqueous solubility, using a phosphatidylcholine (PC)-based solid dispersion system. When fabricating the PC-based solid dispersion, we employed mesoporous microparticles, as an adsorbent, and disintegrants to improve the sticky nature of PC and dissolution of aprepitant, respectively. The solid dispersions were prepared by a solvent evaporation technique and characterized by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry, and X-ray powder diffraction. The FTIR results showed that aprepitant interacted with the PC carrier by both hydrogen bonds and van der Waals forces that can also be observed in the interaction between aprepitant and polymer carriers. The solid dispersions fabricated with only PC were not sufficient to convert the crystallinity of aprepitant to an amorphous state, whereas the formulations that included adsorbent and disintegrant successfully changed that of aprepitant to an amorphous state. Both the solubility and dissolution of aprepitant were considerably enhanced in the PC-based solid dispersions containing adsorbent and disintegrant compared with those of pure aprepitant and polymer-based solid dispersions. Therefore, these results suggest that our PC-based solid dispersion system is a promising alternative to conventional formulations for poorly water-soluble drugs, such as aprepitant.
Towards a Quantitative Mechanistic Understanding of Localized Pulmonary Tissue Retention—A Combined In Vivo/In Silico Approach Based on Four Model Drugs
Authors: Himstedt Anneke , Braun Clemens , Wicha Sebastian Georg , Borghardt Jens Markus
Published: April 2020

Increasing affinity to lung tissue is an important strategy to achieve pulmonary retention and to prolong the duration of effect in the lung. As the lung is a very heterogeneous organ, differences in structure and blood flow may influence local pulmonary disposition. Here, a novel lung preparation technique was employed to investigate regional lung distribution of four drugs (salmeterol, fluticasone propionate, linezolid, and indomethacin) after intravenous administration in rats. A semi-mechanistic model was used to describe the observed drug concentrations in the trachea, bronchi, and the alveolar parenchyma based on tissue specific affinities (Kp) and blood flows. The model-based analysis was able to explain the pulmonary pharmacokinetics (PK) of the two neutral and one basic model drugs, suggesting up to six-fold differences in Kp between trachea and alveolar parenchyma for salmeterol. Applying the same principles, it was not possible to predict the pulmonary PK of indomethacin, indicating that acidic drugs might show different pulmonary PK characteristics. The separate estimates for local Kp, tracheal and bronchial blood flow were reported for the first time. This work highlights the importance of lung physiology- and drug-specific parameters for regional pulmonary tissue retention. Its understanding is key to optimize inhaled drugs for lung diseases.
A Silk Fibroin Based Hydration Accelerator for Root Canal Filling Materials
Authors: Huang Ching-Shuan , Hsieh Sung-Chih , Teng Nai-Chia , Lee Wei-Fang , Negi Poonam , Belem Wendimi Fatimata , Wu Hsuan-Chen , Yang Jen-Chang
Published: April 2020

Mineral trioxide aggregate (MTA) is widely used in various dental endodontic applications such as root-end filling, furcal perforation repair, and vital pulp therapy. In spite of many attempts to improve handling properties and reduce the discoloration of MTA, the ideal root canal filling material has yet to be fully developed. The objective of this study was to investigate the setting time, mechanical properties, and biocompatibility of MTA set by a silk fibroin solution. A 5 wt% silk fibroin (SF) solution (a novel hydration accelerant) was used to set SavDen® MTA and ProRoot® white MTA (WMTA). Changes in setting time, diametral tensile strength (DTS), material crystallization, in vitro cell viability, and cell morphology were assessed by Vicat needle measurement, a universal testing machine, scanning electron microscopy (SEM), and WST-1 assay, respectively. The initial setting time of ProRoot® MTA and SavDen® MTA experienced a drastic decrease of 83.9% and 42.1% when deionized water was replaced by 5 wt% SF solution as the liquid phase. The DTS of SavDen® MTA showed a significant increase after set by the SF solution in 24 h. A human osteoblast-like cell (MG-63)-based WST-1 assay revealed that both ProRoot® MTA and SavDen® MTA hydrated using SF solution did not significantly differ (p > 0.05) in cell viability. MG-63 cells with pseudopodia attachments and nuclear protrusions represent a healthier and more adherent status on the surface of MTA when set with SF solution. The results suggest that the 5 wt% SF solution may be used as an alternative hydration accelerant for MTA in endodontic applications.
Copolymacrolactones Grafted with l-Glutamic Acid: Synthesis, Structure, and Nanocarrier Properties
Authors: Tinajero-Díaz Ernesto , Martínez de Ilarduya Antxon , Muñoz-Guerra Sebastián
Published: April 2020

The enzymatic ring-opening copolymerization (eROP) of globalide (Gl) and pentadecalactone (PDL) was performed in solution from mixtures of the two macrolactones at ratios covering the whole range of comonomeric compositions. The resulting P(Glx-r-PDLy) random copolyesters were aminofunctionalized by thiol-ene reaction with aminoethanethiol. ROP of γ-benzyl-l-glutamate N-carboxyanhydride initiated by P(Glx-r-PDLy)-NH2 provided neutral poly(γ-benzyl-l-glutamate)-grafted copolyesters, which were converted by hydrolysis into negatively charged hybrid copolymers. Both water-soluble and nonsoluble copolymers were produced depending on copolymer charge and their grafting degree, and their capacity for self-assembling in nano-objects were comparatively examined. The emulsion solvent-evaporation technique applied to the chloroform-soluble copolymers grafted with benzyl glutamate rendered well-delineated spherical nanoparticles with an average diameter of 200–300 nm. Conversely, micellar solutions in water were produced from copolyesters bearing grafted chains composed of at least 10 units of glutamic acid in the free form. The copolymer micelles were shown to be able to load doxorubicin (DOX) efficiently through electrostatic interactions and also to release the drug at a rate that was markedly pH dependent.
A Novel Acetylene-Functional/Silicon-Containing Benzoxazine Resin: Preparation, Curing Kinetics and Thermal Properties
Authors: Mei Qilin , Wang Honghua , Tong Danliao , Song Jiuqiang , Huang Zhixiong
Published: April 2020

Benzoxazine resin has been paid more attention in the fields of aviation, electronics, automobiles and new energy industries because of its excellent comprehensive performance. Further application is limited, however, by shortcomings such as high brittleness and high curing temperature. Furthermore, higher thermal stability is imperiously demanded in special areas. Incorporating both an acetylene group and silicon into the benzoxazine monomer is a promising possible solution to improve the curing processability, thermal properties and toughness of benzoxazine. In this paper, an acetylene-functional/silicon-containing benzoxazine monomer was prepared by two-step synthesis, and acetylene-functional benzoxazine was also prepared as a comparison. FTIR and 1H NMR confirmed the molecular structure of acetylene-functional/silicon-containing benzoxazine. Differential scanning calorimetry (DSC) analysis showed that the initial and peak degradation temperatures of acetylene-functional/silicon-containing benzoxazine were decreased by 21 °C and 18 °C compared with acetylene-functional benzoxazine, respectively. The apparent activation energy of the curing reaction of acetylene-functional/silicon-containing benzoxazine was 83.1 kJ/mol, which was slightly lower than acetylene-functional benzoxazine (84.7 kJ/mol). TGA results showed that the acetylene-functional/silicon-containing benzoxazine had a higher thermal stability than acetylene-functional benzoxazine. The temperatures of 5% weight loss of acetylene-functional/silicon-containing benzoxazine were 380 °C in nitrogen and 485 °C in air, and the char yield at 1000 °C was 80% in nitrogen and 21% in air, respectively. The results of mechanical properties showed that the impact strength of acetylene-functional/silicon-containing benzoxazine was higher than acetylene-functional benzoxazine by 35.4%. The tensile and flexural strengths of acetylene-functional/silicon-containing benzoxazine were slightly higher than that of acetylene-functional benzoxazine.
Marine-Derived Polymeric Materials and Biomimetics: An Overview
Authors: Claverie Marion , McReynolds Colin , Petitpas Arnaud , Thomas Martin , Fernandes Susana C. M.
Published: April 2020

The review covers recent literature on the ocean as both a source of biotechnological tools and as a source of bio-inspired materials. The emphasis is on marine biomacromolecules namely hyaluronic acid, chitin and chitosan, peptides, collagen, enzymes, polysaccharides from algae, and secondary metabolites like mycosporines. Their specific biological, physicochemical and structural properties together with relevant applications in biocomposite materials have been included. Additionally, it refers to the marine organisms as source of inspiration for the design and development of sustainable and functional (bio)materials. Marine biological functions that mimic reef fish mucus, marine adhesives and structural colouration are explained.
Recent Advances in Hybrid Biomimetic Polymer-Based Films: from Assembly to Applications
Authors: Krywko-Cendrowska Agata , di Leone Stefano , Bina Maryame , Yorulmaz-Avsar Saziye , Palivan Cornelia G. , Meier Wolfgang
Published: April 2020

Biological membranes, in addition to being a cell boundary, can host a variety of proteins that are involved in different biological functions, including selective nutrient transport, signal transduction, inter- and intra-cellular communication, and cell-cell recognition. Due to their extreme complexity, there has been an increasing interest in developing model membrane systems of controlled properties based on combinations of polymers and different biomacromolecules, i.e., polymer-based hybrid films. In this review, we have highlighted recent advances in the development and applications of hybrid biomimetic planar systems based on different polymeric species. We have focused in particular on hybrid films based on (i) polyelectrolytes, (ii) polymer brushes, as well as (iii) tethers and cushions formed from synthetic polymers, and (iv) block copolymers and their combinations with biomacromolecules, such as lipids, proteins, enzymes, biopolymers, and chosen nanoparticles. In this respect, multiple approaches to the synthesis, characterization, and processing of such hybrid films have been presented. The review has further exemplified their bioengineering, biomedical, and environmental applications, in dependence on the composition and properties of the respective hybrids. We believed that this comprehensive review would be of interest to both the specialists in the field of biomimicry as well as persons entering the field.
Rotational Molding of Linear Low-Density Polyethylene Composites Filled with Wheat Bran
Authors: Hejna Aleksander , Barczewski Mateusz , Andrzejewski Jacek , Kosmela Paulina , Piasecki Adam , Szostak Marek , Kuang Tairong
Published: April 2020

Application of lignocellulosic fillers in the manufacturing of wood polymer composites (WPCs) is a very popular trend of research, however it is still rarely observed in the case of rotational molding. The present study aimed to analyze the impact of wheat bran content (from 2.5 wt.% to 20 wt.%) on the performance of rotationally-molded composites based on a linear low-density polyethylene (LLDPE) matrix. Microscopic structure (scanning electron microscopy), as well as physico-mechanical (density, porosity, tensile performance, hardness, rebound resilience, dynamic mechanical analysis), rheological (oscillatory rheometry) and thermo-mechanical (Vicat softening temperature) properties of composites were investigated. Incorporation of 2.5 wt.% and 5 wt.% of wheat bran did not cause significant deterioration of the mechanical performance of the material, despite the presence of ‘pin-holes’ at the surface. Values of tensile strength and rebound resilience were maintained at a very similar level, while hardness was slightly decreased, which was associated with the porosity of the structure. Higher loadings resulted in the deterioration of mechanical performance, which was also expressed by the noticeable rise of the adhesion factor. For lower loadings of filler did not affect the rheological properties. However, composites with 10wt.% and 20 wt.% also showed behavior suitable for rotational molding. The presented results indicate that the manufacturing of thin-walled products based on wood polymer composites via rotational molding should be considered a very interesting direction of research.
Electrorheological Behavior of Suspensions of Polyimide-Based on the Sodium Salt of 2,5-Diaminobenzenesulfonic Acid
Authors: Semenov Nikolay , Danilin Alexander , Karnet Yulia , Kelbysheva Elena
Published: April 2020

Electrorheological suspensions (ERS) of polyimide particles with organoelement fragments from the sodium salt of 2,5-diaminobenzene sulfonic acid were obtained for the first time. Their rheological and electrorheological characteristics, their dependence on the parameters of deformation, and the intensity of the external electric field were studied. It was found that the ERS of PI-Na polyimide particles have a significant electrorheological response. Solid-polyimide materials were previously studied using a scanning electron microscope. The effect of the polyimide concentration on the properties of the solution was studied by spectrophotometry. It was shown that polyimide suspension is a result of increasing intensity as the electric field changes the flow type from Newtonian to pseudoplastic due to polarization of the particles and formation of the chain structures along the power lines of the electric field. The influence of temperature on the change of rheological and electrorheological properties of a polyimide ERS in constant electric fields was investigated.
Assessing Soil Cover Levels during the Non-Growing Season Using Multitemporal Satellite Imagery and Spectral Unmixing Techniques
Authors: Laamrani Ahmed , Joosse Pamela , McNairn Heather , Berg Aaron , Hagerman Jennifer , Powell Kathryn , Berry Mark
Published: April 2020

Growing cover or winter crops and retaining crop residue on agricultural lands are considered beneficial management practices to address soil health and water quality. Remote sensing is a valuable tool to assess and map crop residue cover and cover crops. The objective of this study is to evaluate the performance of linear spectral unmixing for estimating soil cover in the non-growing season (November–May) over the Canadian Lake Erie Basin using seasonal multitemporal satellite imagery. Soil cover ground measurements and multispectral Landsat-8 imagery were acquired for two areas throughout the 2015–2016 non-growing season. Vertical soil cover photos were collected from up to 40 residue and 30 cover crop fields for each area (e.g., Elgin and Essex sites) when harvest, cloud, and snow conditions permitted. Images and data were reviewed and compiled to represent a complete coverage of the basin for three time periods (post-harvest, pre-planting, and post-planting). The correlations between field measured and satellite imagery estimated soil covers (e.g., residue and green) were evaluated by coefficient of determination (R2) and root mean square error (RMSE). Overall, spectral unmixing of satellite imagery is well suited for estimating soil cover in the non-growing season. Spectral unmixing using three-endmembers (i.e., corn residue-soil-green cover; soybean residue-soil-green cover) showed higher correlations with field measured soil cover than spectral unmixing using two- or four-endmembers. For the nine non-growing season images analyzed, the residue and green cover fractions derived from linear spectral unmixing using corn residue-soil-green cover endmembers were highly correlated with the field-measured data (mean R2 of 0.70 and 0.86, respectively). The results of this study support the use of remote sensing and spectral unmixing techniques for monitoring performance metrics for government initiatives, such as the Canada-Ontario Lake Erie Action Plan, and as input for sustainability indicators that both require knowledge about non-growing season land management over a large area.
Measurement and Modeling of Microbial Growth Using Timelapse Video
Authors: Delibasis Konstantinos , Basanou Ifigenia , Boulogeorgos Alexandros-Apostolos A.
Published: April 2020

The development of timelapse videos for the investigation of growing microbial colonies has gained increasing interest due to its low cost and complexity implementation. In the present study, a simple experimental setup is proposed for periodic snapshot acquisition of a petri dish cultivating a fungus of the genus Candida SPP, thus creating a timelapse video. A computational algorithm, based on image processing techniques is proposed for estimating the microbial population and for extracting the experimental population curves, showing the time evolution of the population of microbes at any region of the dish. Likewise, a novel mathematical population evolution modeling approach is reported, which is based on the logistic function (LF). Parameter estimation of the aforementioned model is described and visually assessed, in comparison with the conventional and widely-used LF method. The effect of the image analysis parameterization is also highlighted. Our experiments take into account different area sizes, i.e., the number of pixels in the neighborhood, to generate population curves and calculate the model parameters. Our results reveal that, as the size of the area increases, the curve becomes smoother, the signal-to-noise-ratio increases and the estimation of model parameters becomes more accurate.
Fully Quantum String Representation of a Wilson Loop in the Finite-Temperature 3D Yang–Mills Theory
Authors: Antonov Dmitry
Published: April 2020

We demonstrate the emergence of the Polchinski–Strominger term in the string representation of a Wilson loop in the confinement phase of the finite-temperature 3D Yang–Mills theory. At a temperature which is roughly twice smaller than the deconfinement critical temperature, the value of the coupling of that term becomes such that the string conformal anomaly cancels out, thereby admitting a fully quantum description of the quark–antiquark string in 3D rather than 26D.
Efficient Method for Molecular Characterization of the 5′ and 3′ Ends of the Dengue Virus Genome
Authors: Rosales-Munar Alicia , Alvarez-Diaz Diego Alejandro , Laiton-Donato Katherine , Peláez-Carvajal Dioselina , Usme-Ciro Jose A.
Published: April 2020

Dengue is a mosquito-borne disease that is of major importance in public health. Although it has been extensively studied at the molecular level, sequencing of the 5′ and 3′ ends of the untranslated regions (UTR) commonly requires specific approaches for completion and corroboration. The present study aimed to characterize the 5′ and 3′ ends of dengue virus types 1 to 4. The 5′ and 3′ ends of twenty-nine dengue virus isolates from acute infections were amplified through a modified protocol of the rapid amplification cDNA ends approach. For the 5′ end cDNA synthesis, specific anti-sense primers for each serotype were used, followed by polyadenylation of the cDNA using a terminal transferase and subsequent PCR amplification with oligo(dT) and internal specific reverse primer. At the 3′ end of the positive-sense viral RNA, an adenine tail was directly synthetized using an Escherichia coli poly(A) polymerase, allowing subsequent hybridization of the oligo(dT) during cDNA synthesis. The incorporation of the poly(A) tail at the 5′ and 3′ ends of the dengue virus cDNA and RNA, respectively, allowed for successful primer hybridization, PCR amplification and direct sequencing. This approach can be used for completing dengue virus genomes obtained through direct and next-generation sequencing methods.
River Channel Forms in Relation to Bank Steepness: A Theoretical Investigation Using a Variational Analytical Method
Authors: Fan Jinsheng , Huang Heqing , Yu Guoan , Su Teng
Published: April 2020

Riverbanks vary considerably in anti-scourability and consequently take various profiles. By using an isosceles trapezoid as the generalized form of river channel cross-sections and then incorporating the effects of bank angle into the variational analytical approach developed by Huang and Nanson (2000), this study presents a detailed theoretical investigation of the self-adjustment of alluvial channel forms. It is demonstrated that when alluvial channel flow achieves stable equilibrium, a significant decrease in riverbank steepness leads to a slight decrease in maximum sediment (bedload) discharge, and yet results in a significant increase in optimal channel width and a considerable decrease in optimal channel depth. The hydraulic geometry relations, theoretically derived for bank steepness to vary across a wide range, show that among the multivariant controls, the roles of bed sediment size, channel roughness, flow discharge and sediment (bedload) discharge are independent of bank steepness. While the effects of bank steepness illustrated in the theoretically derived hydraulic geometry relations are highly consistent with the results of threshold theory and previous empirical studies, limitations on using bank angle to reflect the anti-scourability of natural riverbanks are also highlighted.
Responsible Water Reuse Needs an Interdisciplinary Approach to Balance Risks and Benefits
Authors: Dingemans Milou , Smeets Patrick , Medema Gertjan , Frijns Jos , Raat Klaasjan , van Wezel Annemarie , Bartholomeus Ruud
Published: April 2020

Freshwater is a precious resource, and shortages can lead to water stress, impacting agriculture, industry, and other sectors. Wastewater reuse is increasingly considered as an opportunity to meet the freshwater demand. Legislative frameworks are under development to support the responsible reuse of wastewater, i.e., to balance benefits and risks. In an evaluation of the proposed European regulation for water reuse, we concluded that the proposed regulation is not practically feasible, as the water provider alone is responsible for the risk assessment and management, even beyond their span of control. The required knowledge and resources are extensive. Therefore, without clear guidance for implementation, the regulation would hinder implementation of reuse programs. As a consequence, the current practice of uncontrolled, unintentional, and indirect reuse continues, including related risks and inefficiency. Therefore, we provide an outline of the interdisciplinary approach required to design and achieve safe, responsible water reuse. Responsible water reuse requires knowledge of water demand and availability, quality and health, technology, and governance for the various types of application. Through this paper we want to provide a starting point for an interdisciplinary agenda to compile and generate knowledge (databases), approaches, guidelines, case examples, codes of practice, and legislation to help bring responsible water reuse into practice.
Structural analysis of the northern Nagssugtoqidian orogen, West Greenland: an example of complex tectonic patterns in reworked high-grade metamorphic terrains
Authors: Mazur Stanislaw , Piazolo Sandra , Alsop G. Ian
Published: April 2020

Structural analysis of the deeply eroded northern flank of the Palaeoproterozoic Nagssugtoqidian orogen shows marked regional variations in both the orientation and type of fabrics, as is characteristic of Precambrian high-grade terrains subjected to polyphase deformation. Here we investigate the relationship between strain, metamorphic grade, and the resulting structural patterns. The study area south of Aasiaat in West Greenland consists of amphibolite- to granulite-grade Archaean orthogneisses and relatively thin supracrustal units. The regional foliation displays a WSW–ENE to SW–NE strike associated with steep to moderate dips towards the WNW or SSE. Lineation trends are WSW–ENE and generally plunge gently towards the WSW. Mesoscopic fold hinges are usually colinear with the regional lineation. A systematic change in the plunge of lineations occurs across the south-western part of the study area. Towards the south, the lineation plunge progressively increases, despite the generally uniform strike of foliation. This southward increase of lineation pitch is typically associated with the transition from L > S or L = S shape fabrics in rocks characterised by a low pitch, to S > L or S fabrics in the zone of moderate to high pitch. The structural patterns point to subdivision of the study area into a southern domain mostly characterised by S or S > L shape fabrics and a moderate to high angle of lineation pitch, and a northern domain showing L > S or L = S fabrics and low angles of lineation pitch. This subdivision corresponds well with the map scale boundary between granulite facies rocks in the south and amphibolite facies rocks farther north. The observed structural pattern may be explained by two alternative tectonic models: (1) northward indentation of the previously cooled granulite block into the rheologically weaker amphibolite domain, and (2) strain partitioning within a mid-crustal transpression zone. In model 2 the northern domain represents a localised zone dominated by strike-slip kinematics, whereas the southern domain shows evidence of mostly coaxial shortening. Recent geochronology supports the indentator model in spite of limited available data. Despite the details and structural complexities of the two tectonic models, the granulite and amphibolite facies domains seem to form autochthonous segments of a crustal section linked by a transitional zone that was only reactivated and reworked during indentation or transpression. The Nagssugtoqidian compression was effectively transferred across this zone towards the northern amphibolite domain that suffered penetrative deformation during the Palaeoproterozoic event. The N–S shortening was accommodated through folding, indentation and/or strike-slip displacements, rather than by thrusting and folding as seen south of the study area.
Regional climate-model performance in Greenland firn derived from in situ observations
Authors: Charalampidis Charalampos , Van As Dirk , Langen Peter L. , Fausto Robert S. , Vandecrux Baptiste , Box Jason E.
Published: April 2020

Recent record-warm summers in Greenland (Khan et al. 2015) have started affecting the higher regions of the ice sheet (i.e. the accumulation area), where increased melt has altered the properties of firn (i.e. multi-year snow). At high altitudes, meltwater percolates in the porous snow and firn, where it refreezes. The result is mass conservation, as the refrozen meltwater is essentially stored (Harper et al. 2012). However, in some regions increased meltwater refreezing in shallow firn has created thick ice layers. These ice layers act as a lid, and can inhibit meltwater percolation to greater depths, causing it to run off instead (Machguth et al. 2016). Meltwater at the surface also results in more absorbed sunlight, and hence increased melt in the accumulation area (Charalampidis et al. 2015). These relatively poorly understood processes are important for ice-sheet mass-budget projections.
The Greenland Mineral Resources Portal – another step forward
Authors: Pedersen Mikael , Hansen Martin , Heincke Bjørn H. , Thorning Leif
Published: April 2020

In April 2015, the Geological Survey of Denmark and Greenland (GEUS) together with the Ministry of Mineral Resources in Greenland (MMR) made further progress in the development of web-based facilities to present and disseminate geoscientific information and data. This was presented in a new version of the Greenland Mineral Resources Portal. The portal now provides the users with access to a wealth of geological, geophysical and geochemical data – mostly free of charge (Fig. 1). The primary goal of the portal is to facilitate data searches for exploration companies, but the benefits from the easy access to geoscience data are also open to academic researchers. It is the plan to add several new functionalities to the portal in 2016, and interested users are invited to visit the portal at www. greenmin.gl on a regular basis to follow progress.
aFieldWork – an Android app for offline recording of geological information and data display
Authors: Hansen Martin , Nelleman Petersen Matti , Kokfelt Thomas F. , Møller Stensgaard Bo
Published: April 2020

Since 2008 the Geological Survey of Denmark and Greenland (GEUS) has used handheld devices (Personal Digital Assistents PDAs) for collecting field data in a digital format. Since PDAs are becoming obsolete and new device technology with improved functionality is available, it was decided to develop an Android-based application (app) that can be used by many mobile telephones and to test this on different devices during field work in Greenland.