bluuebunny/crossref_metadata_2025 huggingface dataset:
Broad cognitive complaints but subtle objective working memory impairment in fibromyalgia patients
Authors: Pidal-Miranda Marina , González-Villar Alberto Jacobo , Carrillo-de-la-Peña María Teresa , Andrade Elena , Rodríguez-Salgado Dolores
Published: November 2018

Background

Cognitive dysfunction in fibromyalgia (FM) encompasses objective cognitive difficulties, as measured in neuropsychological tests, and self-reported cognitive complaints. Although it has been suggested that FM patients display problems in working memory, the data are inconsistent, and the overall working memory status of the patients is unclear. It is also not clear whether the working memory problems are related to cognitive complaints or how the dyscognition is affected by the characteristic clinical symptoms of FM.

Methods

To clarify these aspects, we explored the neuropsychological performance for different components of working memory and the subjective self-perception of cognitive status in a sample of 38 women with FM. They were compared with a matched group of 32 healthy women.

Results

Our findings suggested that the FM patients do not differ from healthy controls in their overall working memory functioning. Only a poor performance was found in a single task of visuospatial working memory, mediated by the presence of depressive symptoms, fatigue and pain. The FM patients also displayed a higher level of perception of cognitive difficulties than healthy controls, and this difference was mediated by depression and fatigue. Furthermore, cognitive complaints in FM patients were only associated with a lower verbal WM capacity.

Discussion

FM patients have a subtle specific impairment in their working memory functioning, as well as elevated concern about their cognitive status. These findings suggest a disconnection between neuropsychological performance and subjective complaints. In FM patients, clinical variables such as pain, fatigue, and depression play an important role in dyscognition, as assessed by both objective and subjective measures, and should be taken into account in future research.
Catch fast and kill quickly: do tiger beetles use the same strategies when hunting different types of prey?
Authors: Rewicz Tomasz , Jaskuła Radomir
Published: November 2018

Background

Tiger beetles (Coleoptera: Cicindelidae) are fast running predatory insects preying on different small insects and other terrestrial arthropods. Prey is located by sight and captured after short and fast pursuit interspersed with pause-and-look behaviour. At least some tiger beetle species can recognise the size and location of prey using memory, which probably allows them to achieve greater hunting success.

Material and Methods

Two eurytopic tiger beetle species known to occur in different types of habitat were used in the study:Cicindela hybrida hybrida, a very common central European beetle found even in artificial habitats such as sandy roads or gravel pits, andCalomera littoralis nemoralis, a species widely distributed in southern European countries and occurring on sandy sea beaches, in salt marshes, as well as on sandy banks of rivers and lakes. Both species are very similar in body size. Specimens used in the study were collected in the field and later tested in the laboratory. We checked whether tiger beetles use different hunting strategies when attacking prey of different sizes and abilities to escape as well as whether the sex of the studied species makes a difference in its hunting behaviour.

Results

The hunting strategies of both tiger beetle species consist of the following main phases: identification, pursuit (often with stops), attack, and optional release of the prey, and then the secondary attack, abandonment of the prey, or consumption of the prey. Considerable differences were noticed in hunting behaviour depending on the type of prey, its movement ability and escape potential. Caterpillars were attacked without pursuit, in the head or directly behind the head where a concentration of nerves and main muscles responsible for walking are located. Effective attacks on beetles were executed at the connection between the thorax and the abdomen.Calomera littoralisstrongly preferred slow moving prey, whileCicindela hybridapreferred in equal measure slow moving prey and medium-sized fast moving prey. The experiment on the preferred size of prey indicated small beetles and small caterpillars as favoured byCalomera littoralis, whileCicindela hybridapreferred medium-sized fast moving prey and large caterpillars.

Discussion

The hunting behaviour ofCalomera littoralisandCicindela hybridais complicated and includes a number of phases allowing to locate, capture and kill the prey. Beetles are able to discriminate between different types of prey and apply different behavioural tactics to hunt it. As the particular strategies are used to increase hunting success, and as a result allow to accumulate energy for future activity of the predator, it can be expected that such a type of hunting behaviour is characteristic also of other tiger beetle species.
Δ12 fatty acid desaturase gene from Geotrichum candidum in cheese: molecular cloning and functional characterization
Authors: Luo Xue , Shi Haisu , Wu Rina , Wu Junrui , Pi Yuzhen , Zheng Yan , Yue Xiqing
Published: November 2018

Soft cheese with white rind lacks essential fatty acids (EFAs), and as a result its long‐term consumption may lead to various kinds of cardiovascular and cerebrovascular diseases, such as hyperlipidemia, hypertension, and atherosclerosis. Geotrichum candidum is a dimorphic yeast that plays an important role in the ripening of mold cheese. A gene coding for Δ12 fatty acid desaturase, a critical bifunctional enzyme desaturating oleic acid (OA) and linoleic acid (LA) to produce LA and α‐linolenic acid (ALA), respectively, was isolated from G. candidum, and then cloned and heterologously expressed in Saccharomyces cerevisiae. This gene, named GcFADS12, had an open reading frame of 1257 bp and codes for a protein of 419 amino acids with a predicted molecular mass of 47.5 kDa. Characterization showed that GcFADS12 had the ability to convert OA to LA and LA to ALA, and the conversion rates for OA and LA were 20.40 ± 0.66% and 6.40 ± 0.57%, respectively. We also found that the protein product of GcFADS12 catalyzes the conversion of the intermediate product (LA) to ALA by addition of OA as the sole substrate. The catalytic activity of GcFADS12 on OA and LA was unaffected by fatty acid concentrations. Kinetic analysis revealed that GcFADS12 had stronger affinity for the OA than for the LA substrate. This study offers a solid basis for improving the production of EFAs by G. candidum in cheese.
Wild‐type p53 regulates OTOP2 transcription through DNA loop alteration of the promoter in colorectal cancer
Authors: Qu Huajun , Su Yi , Yu Lianzhi , Zhao Hongchao , Xin Chunxia
Published: November 2018

Colorectal cancer (CRC) is the third most commonly diagnosed malignancy worldwide and remains a major public health issue. Therefore, further investigation is required to delineate the cellular and molecular mechanisms underlying colorectal tumorigenesis. Using CRC data taken from The Cancer Genome Atlas, we determined that the expression of otopetrin 2 (OTOP2) is highly correlated with malignancy grade and rate of patient survival. Here, we report that OTOP2 is down‐regulated in cancerous tissues and that elevated OTOP2 effectively suppresses tumor proliferation in vitro. We demonstrate that wild‐type p53 (wtp53), but not mutant p53 (mtp53), can regulate the transcription of otop2 in CRC cells. Subsequently, we investigate the chromatin architecture of the otop2 promoter, whereby we discover alterations in p53‐dependent DNA loop organization and CCCTC‐binding factor (CTCF) binding between cells with wtp53 and mtp53. In conclusion, our study promotes an in‐depth understanding of tumorigenesis, which may also lead to the development of therapeutic applications targeting human malignancy.
MXene‐Enabled Electrochemical Microfluidic Biosensor: Applications toward Multicomponent Continuous Monitoring in Whole Blood
Authors: Liu Jiang , Jiang Xiantao , Zhang Ruyue , Zhang Yang , Wu Leiming , Lu Wei , Li Jianqing , Li Yingchun , Zhang Han
Published: November 2018

Continuous and real‐time sensoring has received much attention in biomarker monitoring, toxicity assessment, and therapeutic agent tracking. However, its on‐site application is seriously limited by several stubborn defects including liability to fouling, signal drifting, short service life, poor repeatability, etc. Additionally, most current methods require extra sample pretreatment, delaying timely acquisition of testing results. To address these issues, MXene‐Ti3C2Tx based screen‐printed electrode incorporated with a dialysis microfluidic chip is constructed for a direct and continuous multicomponent analysis of whole blood. Dual‐function of MXene is developed and allows for simultaneous quantification of different target compounds through one device. Importantly, ratiometric sensing tactic is easily implemented in the system, which greatly alleviates signal drifting. As a proof of concept, this novel sensor is applied in hemodialysis, and continuous assay of urea, uric acid, and creatinine levels in human blood is realized. This work paves a new path for 2D MXene in biomedical and sensing applications.
Quasi‐Hodgkin–Huxley Neurons with Leaky Integrate‐and‐Fire Functions Physically Realized with Memristive Devices
Authors: Huang He‐Ming , Yang Rui , Tan Zheng‐Hua , He Hui‐Kai , Zhou Wen , Xiong Jue , Guo Xin
Published: November 2018

Artificial neurons with functions such as leaky integrate‐and‐fire (LIF) and spike output are essential for brain‐inspired computation with high efficiency. However, previously implemented artificial neurons, e.g., Hodgkin–Huxley (HH) neurons, integrate‐and‐fire (IF) neurons, and LIF neurons, only achieve partial functionality of a biological neuron. In this work, quasi‐HH neurons with leaky integrate‐and‐fire functions are physically demonstrated with a volatile memristive device, W/WO3/poly(3,4‐ethylenedioxythiophene): polystyrene sulfonate/Pt. The resistive switching behavior of the device can be attributed to the migration of protons, unlike the migration of oxygen ions normally involved in oxide‐based memristors. With multifunctions similar to their biological counterparts, quasi‐HH neurons are advantageous over the reported HH and LIF neurons, demonstrating their potential for neuromorphic computing applications.
One‐Step Generation of a Drug‐Releasing Hydrogel Microarray‐On‐A‐Chip for Large‐Scale Sequential Drug Combination Screening
Authors: Song Seo Woo , Kim Su Deok , Oh Dong Yoon , Lee Yongju , Lee Amos Chungwon , Jeong Yunjin , Bae Hyung Jong , Lee Daewon , Lee Sumin , Kim Jiyun , Kwon Sunghoon
Published: November 2018

Large‐scale screening of sequential drug combinations, wherein the dynamic rewiring of intracellular pathways leads to promising therapeutic effects and improvements in quality of life, is essential for personalized medicine to ensure realistic cost and time requirements and less sample consumption. However, the large‐scale screening requires expensive and complicated liquid handling systems for automation and therefore lowers the accessibility to clinicians or biologists, limiting the full potential of sequential drug combinations in clinical applications and academic investigations. Here, a miniaturized platform for high‐throughput combinatorial drug screening that is “pipetting‐free” and scalable for the screening of sequential drug combinations is presented. The platform uses parallel and bottom‐up formation of a heterogeneous drug‐releasing hydrogel microarray by self‐assembly of drug‐laden hydrogel microparticles. This approach eliminates the need for liquid handling systems and time‐consuming operation in high‐throughput large‐scale screening. In addition, the serial replacement of the drug‐releasing microarray‐on‐a‐chip facilitates different drug exchange in each and every microwell in a simple and highly parallel manner, supporting scalable implementation of multistep combinatorial screening. The proposed strategy can be applied to various forms of combinatorial drug screening with limited amounts of samples and resources, which will broaden the use of the large‐scale screening for precision medicine.
The function of geophagy in Nepal gray langurs: Sodium acquisition rather than detoxification or prevention of acidosis
Authors: Monaco Eliot T. , Borries Carola , Nikolei Julia , Chalise Mukesh K. , Ganzhorn Jörg U. , Wesche Karsten , Koenig Andreas
Published: November 2018

Objectives

Geophagy has several hypothesized functions, among them (1) detoxification of plant secondary compounds, for example, tannins, (2) buffering stomach pH to alleviate acidosis caused by high sugar intake, and (3) supplementing the diet with mineral nutrients. We tested these hypotheses in Nepal gray langurs (Semnopithecus schistaceus), a foregut fermenter for which fruits and leaves dominate the diet at different times of the year.

Materials and Methods

We collected data on feeding time per food item for 21 unprovisioned adult langurs at Ramnagar, Nepal, for 1 year using instantaneous sampling (773 observation hours). We combined these data with relative sugar and tannin content of food items to estimate diet content. We collected rainfall data to distinguish the wet season (May–September) from the dry season (October–April). We collected soil samples from consumption and control sites to test for pH and sodium, potassium, magnesium, and calcium concentrations.

Results

Langurs consumed soil from two sources: termite structures (in almost all months) and river banks and beds (mainly in the dry season). Soil feeding was not positively correlated with dietary tannin or sugar content (GLMM). Soil pH was not high enough to buffer stomach contents. Only sodium was significantly higher in consumed soil.

Discussion

The most likely function of geophagy was the acquisition of sodium. This conclusion is consistent with reports for other animals. Buffering stomach pH, an often‐suggested function for animals with ruminant‐like digestion, was not supported. Detoxification, often proposed for animals with a diet high in secondary compounds, was also not supported.
Model Predictive Control of Nonlinear Discrete Time Systems with Guaranteed Stability
Authors: Shamaghdari Saeed , Haeri Mohammad
Published: November 2018

This paper presents the design of a new robust model predictive control algorithm for nonlinear systems represented by a linear model with unstructured uncertainty. The linear model is obtained by linearizing the nonlinear system at an operating point and the difference between the nonlinear and linear model is considered as a Lipschitz nonlinear function. The controller is designed for the linear model, which fulfills the stabilization condition for the nonlinear term. Unlike previous studies that have not considered a valid Lipschitz matrix of nonlinear term in the design process, we propose an algorithm in this paper in which it is considered. Therefore, the closed loop stability of the nonlinear system is guaranteed. A novel SOS optimization problem to determine design parameters is introduced, which leads to improved closed‐loop performance in comparison to a trial and error tuning procedure. Furthermore, an algorithm is presented to enlarge the region of attraction for the nonlinear closed‐loop system. Stability is improved by checking some additional conditions if which the system may be unstable if not considered. The validity of the proposed algorithm is confirmed by examples.
Systemic and local hepcidin as emerging and important peptides in renal homeostasis and pathology
Authors: Vela Driton
Published: November 2018

Recent data suggest that the importance of hepcidin goes beyond its classical role in controlling systemic iron metabolism. Local hepcidins are emerging as important peptides for organ homeostasis in the brain, heart, blood vessels, and in cancer as well. Similarly, accumulating data indicate that hepcidin does seem to be an important factor in renal homeostasis. This review encompasses present knowledge concerning the role of hepcidin in renoprotection and its use as a biomarker of kidney diseases. Understanding the role of hepcidin in kidneys is important due to its relevance for kidney physiology and its potential therapeutic application in kidney pathologies. © 2018 BioFactors, 45(2):118–134, 2019
Introducing national healthcare safety investigation bodies
Authors: Wiig S , Macrae C
Published: November 2018

Making surgery safer
Nanocrystals: A perspective on translational research and clinical studies
Authors: Jarvis Maria , Krishnan Vinu , Mitragotri Samir
Published: November 2018

Poorly soluble small molecules typically pose translational hurdles owing to their low solubility, low bioavailability, and formulation challenges. Nanocrystallization is a versatile method for salvaging poorly soluble drugs with the added benefit of a carrier‐free delivery system. In this review, we provide a comprehensive analysis of nanocrystals with emphasis on their clinical translation. Additionally, the review sheds light on clinically approved nanocrystal drug products as well as those in development.
Optimal axis for lumbosacral interbody fusion: Prospective finite element analysis and retrospective 3D‐CT measurement
Authors: Li Yang , He Dengwei , Chen Nuo , Lv Ting , Wu Aimin , Lin Zhongke , Ding Zihai , Wang Zhengguo , Wu Lijun
Published: November 2018

A feasible and optimal axis of biomechanical and anatomic significance in axial lumbosacral interbody fusion (AxiaLIF) was designed. Using the image dataset of an adult volunteer, two groups of finite element (FE) models of the AxiaLIF, lumbosacral anterior column fixation (ACF) models and middle column fixation (MCF) models with different bone graft fusion degrees, were prospectively established, and their biomechanical differences were comparatively predicted. In addition, 3D reconstruction was performed by retrospectively collecting CT data from pelvises in 60 adult cases. Their anatomic parameters relating to two groups of models were digitally measured and statistically compared. Numerical analysis revealed that the load and the maximum stress on the screw as well as the maximum stress difference between the screw and peripheral tissues in the MCF model were reduced compared with the ACF model. These indices of both models all decreased markedly in response to the increase in the disc fusion degree. Statistical analysis revealed that the effective fixed length of the sacrum in the MCF model was increased compared with the ACF model (P < 0.05). The surgical dissection distance of presacral vessels and nerves from the axis to sacrum of the MCF model was reduced compared with the ACF model (P < 0.05). The feasible and optimal axis of biomechanical and anatomic significance of the AxiaLIF is similar to the axis of the MCF model. Disc bone graft fusions plus axial screw fixations of middle column could strengthen the biomechanical stability of the AxiaLIF model. Clin. Anat. 32:337–347, 2019. © 2018 Wiley Periodicals, Inc.
Biologically inspired ant colony simulation
Authors: Xiang Wei , Ren Jiaping , Wang Kuan , Deng Zhigang , Jin Xiaogang
Published: November 2018

We present a unified biologically inspired approach to simulate ant colonies inspired by the key observation of collective behaviors of ants in nature. To generate the trajectories of virtual ants, we construct a motion controller to determine the motion states and the paths of virtual ants, considering dynamic internal and external interactions. The motion controller computes a target position for each ant at every time step according to its motion states. The motion states include four states: basic movement, the stop state, and two dynamic interactions (i.e., internal and external, respectively referring to interaction with neighbors for necessary information transfer about the destination, and interaction with surroundings such as food sources, nests, and obstacles) to represent basic exploration, casual or intentional stop, and purposeful movement, respectively. Based on the motion states, the motion controller plans an optimal path for each virtual ant. Through many simulation experiments, we demonstrate that our method is controllable, scalable, and flexible to simulate hybrid colonies with a large number of ants.
Effect of soybean bagasse addition on texture, sensory properties, and protein quality of maize tortillas
Authors: Hernández‐Reyes Karina Elizabeth , Montemayor‐Mora Gabriela , Pérez‐Carrillo Esther , De la Rosa‐Millán Julian , García‐Villanueva Cristina , Serna‐Saldívar Sergio O.
Published: November 2018

Background and objectives

Nixtamalized dry masa flour was supplemented with 5% or 10% dry soybean bagasse (SB) and then processed into table tortillas. Proximate chemical composition, dietary fiber content, affective sensory analyses of corn tortillas prepared from dry masa flour enriched with 5% or 10% dried SB were determined. Also, the in vitro protein digestibility and protein digestibility‐corrected amino acid score (PDCAAS) of the tortillas were evaluated.

Findings

Ten per cent SB addition reduced masa hardness by about 32% and chewiness 47% without affecting adhesiveness. In terms of rapid viscosity analysis, the addition of incremental levels of SB decreased peak and final viscosities 42% and 49%, respectively. The tortilla insoluble and soluble dietary fiber contents increased 50% and fourfold, respectively, while the lysine daily requirement for preschool infants improved from 45.6% to 67.0% when 10% SB was used. Enriched tortillas showed a similar overall acceptance and had improved texture over storage time at room temperature (25% less force to rupture compared with the control tortilla stored for the same time).

Conclusions

The addition of 5% soybean bagasse to nixtamalized corn flour produced masa with good machinability and tortillas with better overall acceptance and textural shelf life and a product with four times more soluble dietary fiber, 10% less starch and with higher amounts of limiting amino acids lysine and tryptophan. The upgraded amino acid balance significantly improved PDCAAS from 41% to 59% in the tortilla containing 10% SB.

Significance and novelty

Soybean bagasse is a viable option to increased fiber and protein quality in nixtamalized corn flour tortillas, with a positive impact on texture during shelf life and consumer acceptance.
Unrecognized staphylococcal vertebral osteomyelitis leading to fatal outcome in a previously healthy patient
Authors: Zayed Yazan , Swaid Bakr , Gupta Rahul , Haykal Tarek , Osterholzer Danielle
Published: November 2018

Key Clinical Message

New onset low back pain which is recalcitrant to usual treatment should be evaluated aggressively even in the absence of fever or neurologic deficits. Corticosteroids given for back pain will accelerate occult spinal infection and may mask symptomatology leading to more severe disease.
Surgical repair of a depressed, comminuted fracture of the zygomatic process of the frontal bone using a locking compression plate in a Thoroughbred colt foal
Authors: Derham Ann M. , Johnson Jessica P. , Kearney Clodagh M. , O’Leary John M.
Published: November 2018

Key Clinical Message

This case report demonstrates the use of a 10‐hole 2.7‐mm locking compression plate (LCP) to repair a depressed, comminuted fracture of the zygomatic process of the frontal bone, in a foal. LCP fixation resulted in excellent cosmesis. The use of LCP fixation in this region has not been previously described.
Individual and organizational learning from inter‐firm knowledge sharing: A framework integrating inter‐firm and intra‐firm knowledge sharing and learning
Authors: Usman Muhammad , Ahmad Malik Imran , Burgoyne John
Published: November 2018

Literature has highlighted but not explored links between knowledge sharing and learning at inter‐firm and intra‐firm levels. Using the single case of an aviation refuelling company as the basis for our research methodology and collecting data through 34 semi‐structured interviews, we develop a framework that integrates knowledge sharing and learning at inter‐firm and intra‐firm levels. We show that intra‐firm knowledge sharing capabilities facilitate the diffusion of inter‐firm learning within organizations. Moreover, inter‐firm trust manifests in different forms that affect individual and organizational learning. The purpose of collaboration determines what a firm learns or discards. The findings are important for organizations facing a shortage of skills. © 2018 ASAC. Published by John Wiley & Sons, Ltd.
Feeding strategies of methanol and lipase on eversa® transform‐mediated hydroesterification for FAME production
Authors: Wancura João H. C. , Rosset Daniela V. , Ugalde Gustavo A. , Oliveira J. Vladimir , Mazutti Marcio A. , Tres Marcus V. , Jahn Sérgio L.
Published: November 2018

This work reports the influence of different feeding strategies of methanol and catalysts on fatty acid methyl esters (FAME) yield in a beef tallow hydroesterification reaction for biodiesel production catalyzed by liquid lipase. Soluble lipase from the Thermomyces lanuginosus microorganism, named Eversa® Transform, was selected for the reactions in this work. The feeding of methanol and lipase into the system was evaluated varying the methanol to fat molar ratio of 4.0:1 and 4.5:1 and the lipase load of 1.0 wt% and 1.45 wt% in relation to the mass of beef tallow utilized in each assay. The highest yield (85.08 %) of FAME was reached at 35 °C, methanol to fat molar ratio of 4.5:1, 1.0 wt% of lipase, 6.0 wt% of water in only 8 h of reaction, with a one‐step addition of lipase and methanol fed to the process at a constant flow of 3.0 g · h−1. Moreover, the reutilization process of the liquid lipase after four cycles was evaluated, which is a point rarely addressed in similar works available in the open literature. The findings of this paper demonstrate that the adoption of an appropriate strategy to feed inputs to the system is fundamental in order to take advantage of the maximum catalytic capacity of the lipase and consequently to obtain high yields in the process.
Evolution of the Ligand Shell Around Small ZnO Nanoparticles During the Exchange of Acetate by Catechol: A Small Angle Scattering Study
Authors: Schindler Torben , Lin Wei , Schmutzler Tilo , Lindner Peter , Peukert Wolfgang , Segets Doris , Unruh Tobias
Published: November 2018

The core‐shell structure of colloids surrounded by ligands is of great importance for their later application as it can significantly alter the chemical and physical properties of the nanoparticles (NPs). A combination of small angle X‐ray and neutron scattering (SAXS/SANS) in the native solution with additional ex situ measurements (titration‐UV) was applied to study the NP/ligand interface of ZnO NPs after functionalization with catechol derivatives. Based on SAXS data, it was found that within the multimodal particle size distribution the fraction of agglomerates is shifted to smaller sizes and nearly disappeared upon the binding of ethyl 3,4‐dihydroxybenzoate (CAT) molecules. This is ascribed to improved stabilization at the primary particle level by CAT molecules. By combining the neutron scattering contrast with the input of bound CAT molecules from a previously developed titration‐UV method, the heterogeneous composition of the ligand shell became accessible for the first time.
Conjugated Alkylamine by Two‐Step Surface Ligand Engineering in CsPbBr3 Perovskite Nanocrystals for Efficient Light‐Emitting Diodes
Authors: Zhang Li , Zhang Qiong , Xing Xiangyu , Jiang Yuanzhi , He Tingwei , Huang Yanmin , Ma Zhiwei , Yang Jien , Yuan Mingjian
Published: November 2018

Long alkyl‐chain capped ligands are prerequisites for preserving high photoluminescence quantum yield (PLQY) in perovskite nanocrystals (NCs). Unfortunately, the insulating feature of surface ligands potentially hinders carrier transport in light‐emitting diode (LED) devices, which will degenerate the LED performance. To overcome this shortcoming, we introduce a conjugated molecule, 3‐phenyl‐2‐propen‐1‐amine (PPA), into CsPbBr3 NCs through a two‐step surface ligand engineering process. Owing to excellent electrical conductivity of PPA, PPA‐capped CsPbBr3 NCs LEDs exhibit a maximum current efficiency of 23.53 cd A−1, and a maximum luminance of 26,475 cd m−2 at the wavelength of 517 nm. This work offers a new strategy to improve the quality of CsPbBr3 NCs and LEDs by introducing conjugation molecular ligands into the all‐inorganic perovskite nanocrystals.
Label‐Free Electrophoretic Mobility Shift Assay (EMSA) for Measuring Dissociation Constants of Protein‐RNA Complexes
Authors: Seo Minguk , Lei Li , Egli Martin
Published: November 2018

The electrophoretic mobility shift assay (EMSA) is a well‐established method to detect formation of complexes between proteins and nucleic acids and to determine, among other parameters, equilibrium constants for the interaction. Mixtures of protein and nucleic acid solutions of various ratios are analyzed via polyacrylamide gel electrophoresis (PAGE) under native conditions. In general, protein–nucleic acid complexes will migrate more slowly than the free nucleic acid. From the distributions of the nucleic acid components in the observed bands in individual gel lanes, quantitative parameters such as the dissociation constant (Kd) of the interaction can be measured. This article describes a simple and rapid EMSA that relies either on precast commercial or handcast polyacrylamide gels and uses unlabeled protein and nucleic acid. Nucleic acids are instead detected with SYBR Gold stain and band intensities established with a standard gel imaging system. We used this protocol specifically to determine Kd values for complexes between the PAZ domain of Argonaute 2 (Ago2) enzyme and native and chemically modified RNA oligonucleotides. EMSA‐based equilibrium constants are compared to those determined with isothermal titration calorimetry (ITC). Advantages and limitations of this simple EMSA are discussed by comparing it to other techniques used for determination of equilibrium constants of protein‐RNA interactions, and a troubleshooting guide is provided. © 2018 by John Wiley & Sons, Inc.
Smear cytological features of large cell neuroendocrine carcinoma of the uterine cervix in pregnancy: A case report and review of the literature
Authors: Erdem Busra Ersan , Çiğdem Vural , Şener Gezer , Bahar Muezzinoglu , Kursat Yıldız Demir , İzzet Yucesoy
Published: November 2018

Large‐cell neuroendocrine carcinoma (LCNEC) of the uterine cervix is a rare aggressive tumor. The examination of a cervicovaginal smear from a 31‐year‐old patient diagnosed with LCNEC after a cervical polypectomy during the 32nd week of pregnancy was carried out. The observed atypical cells had large cytoplasm, increased nucleus: cytoplasm ratio with the nucleus containing coarse, dispersed chromatin, and were arranged in a pseudorosette formation, which all confirmed the diagnosis. In addition, adenocarcinoma in situ (AIS) was determined in the histopathological examination of the subsequent hysterectomy material. Given the rarity of this condition, we present and discuss the case herein.
Organic farming at local and landscape scales fosters biological pest control in vineyards
Authors: Muneret Lucile , Auriol Arthur , Thiéry Denis , Rusch Adrien
Published: November 2018

While organic farming practices, which are often promoted as models of ecological intensification, generally enhance biodiversity, their effects on the delivery of ecosystem services, such as biological pest control, are still unknown. Here, using a multi‐scale hierarchical design in southwestern France, we examined the effects of organic farming and seminatural habitats at the local and landscape scales on biological control services of three pests, including weeds and insects, in 42 vineyards. Organic farming at the local and landscape scales was beneficial to the mean and temporal stability of biological control services, while the proportion of seminatural habitats in the landscape reduced the level of biological pest control potential. The effects of organic farming and seminatural habitats across spatial scales varied with the type of prey considered and with time. Egg moth removal rates were higher in fields under organic management compared to conventional management while weed seed removal rates increased with the proportion of organic farming in the landscape. Larval removal rates as well as seed removal rates were always more stable within time in organic fields than in conventional fields. Moreover, independently of farming system type, local variables describing the agricultural management intensity, such as pesticide use or crop productivity, were also found to be important variables explaining levels of biological control services. Pesticide use tended to reduce biological control potential, while crop productivity was associated with contrasting biological control responses depending on the pest type. Our study demonstrates the need to target multiple spatial scales and to consider farming practices, as well as the proportion of seminatural habitats, to design functional landscapes that optimize biological pest control services.
The effects of a stressed inshore urban reef on coral recruitment in Suva Harbour, Fiji
Authors: Lal Ronal , Kininmonth Stuart , N’Yeurt Antoine D. R. , Riley Ralph H. , Rico Ciro
Published: November 2018

A relic inshore reef ecosystem adjacent to the Fijian capital of Suva and another remote inshore reef were monitored monthly from July 2014 to July 2015 for coral recruitment, sedimentation rates, coral cover, temperature, and light intensity. Despite a major sewage spill in Suva Harbour in December 2014, the municipal inshore site exposed to constant anthropogenic activity, recorded no significant differences in coral spat abundance (except for the family Poritidae) on artificial substrata compared to the remote inshore site. Total yearly spat abundance was 106 on municipal reef and 132 on remote reef, while average daily sediment trap collection rates (g cm2/day) were significantly higher in the municipal site for the entire duration of monitoring. Total annual particulate organic matter content in sediment was also significantly higher in the municipal site (107.51 g cm2), compared to the remote site (43.37 g cm2). Mean light intensity was significantly lower for the municipal site (69.81 lum/ft2) compared to the remote site (239.26 lum/ft2), with Photosynthetically Active Radiation also lower for the former (800–1,066.66 µmol m−2 s−1) compared to the latter (3,266.66–3,600 µmol m−2 s−1). The lack of significant differences in coral spat recruitment rates suggests that settling larvae may be unable to distinguish between sub‐optimal and optimal sites probably as a consequence of interference with coral settlement cues arising from anthropogenic development.
Potential of Nuclear Magnetic Resonance for a Discriminant Characterization of PDO VOOs
Authors: del Caño‐Ochoa Sofía , Ruiz‐Aracama Ainhoa , Guillén Lorén María D.
Published: November 2018

Interest in virgin olive oil (VOO) is growing among consumers and industry due to its well‐received sensorial attributes and to its higher oxidative stability, as well as to its positive health effects. Furthermore, attention is being increasingly paid to other quality attributes of VOO, such as sustainability and geographical origin. For these reasons, this oil has an important added value and is exposed to fraudulent practices. In order to guarantee its origin, the European Union (EU) has developed the Protected Designation of Origin (PDO) quality scheme that links the characteristics of a VOO to its geographical origin. In recent years attempts to develop tools that enable the authentication of PDO‐labeled VOOs are carried out. In this regard, studies using Nuclear Magnetic Resonance (NMR) to this aim are here reviewed.

Practical Applications: Due to the added value of PDO VOOs, their authentication is of great importance for administration, consumers and producers. The studies in which NMR spectroscopy has been used to this aim are analyzed here. In this context, this review provides a view of the state of the art that can be of practical interest not only for the researchers of the subject but also for the aforementioned groups.

Approaches based on NMR spectroscopic data combined with different statistical analysis are used to develop predictive models with the aim of discriminating PDO VOOs from non‐PDO oils, or even to discriminate a VOO from a certain PDO from other different PDO oils.
Diversity Assessment of Algerian Wild and Cultivated Olives (Olea europeae L.) by Molecular, Morphological, and Chemical Traits
Authors: Boucheffa Saliha , Tamendjari Abderezak , Sanchez‐Gimeno Ana Cristina , Rovellini Pierangela , Venturini Stefania , di Rienzo Valentina , Miazzi Monica Marilena , Montemurro Cinzia
Published: November 2018

Algeria has several genetic resources on olive trees, mainly made up of small indigenous cultivars, and a very important wild heritage. Twenty olive samples including eight cultivars and twelve wild trees from the province of Bejaia (Algeria) are characterized, by combining molecular data (13 SSRs), fruit and pit morphological traits, fatty acids composition, and phenolic compounds of the extra virgin olive oils (EVOOs). The genetic results based on PCoA, UPGMA, and AMOVA analyses demonstrate that olive cultivars and wild trees are mixed, suggesting kinship relationships between cultivated and wild olive trees and even cases of synonymy between some cultivars. PCA analysis on morphological traits shows a good separation of the two olive botanical varieties, the wild olive trees producing smaller fruits than those of the cultivated ones. Significant differences are also found in terms of fatty acids and phenol compounds composition of the EVOOs. Wild olive oils show the highest contents on phenolic compounds, mainly oleocanthal, as well as a considerable richness on oleic acid. The comparison of pairwise distances between olive trees obtained by genetic, morphological, fatty acids and phenolic compounds contents data using Mantel's test indicates a significant correlation among morphological characteristics, DNA polymorphism, and phenolic compounds. The results obtained in the present work contribute to reveal the diversity existing in the cultivated and wild olive trees of the region of Bejaia, shedding some light on the importance of Algerian olives germplasm.

Practical Applications: Cultivated and wild olive diversity are assessed by genetic, morphological, fatty acids and phenolic composition. SSR marker analysis demonstrates the presence of a high genetic variation between the analyzed samples. A significant correlation of morphological characteristics with DNA polymorphism and phenolic compounds is found. A significant diversity in the wild and cultivated olive trees is observed.

Kinship relationships between wild and cultivated Algerian olive trees are demonstrated using SSR markers, while the morphological parameters allow a good distinction between the two taxa. Significant differences are found for fatty acids and phenol compounds composition for the EVOOs; the wild olive oils present an interesting composition compared to the cultivated form.
Choosing goals that express the true self: A novel mechanism of the effect of self‐control on goal attainment
Authors: Stavrova Olga , Pronk Tila , Kokkoris Michail D.
Published: November 2018

Why is trait self‐control associated with successful goal progress? Existing research has attempted to answer this question by focusing on individual differences in the process of goal pursuit. Herein, we propose and test a novel mechanism suggesting that self‐control facilitates goal attainment not only by affecting the process of goal pursuit but also the type of goals people select in the first place. Three studies showed that high (vs. low) self‐control individuals are more likely to report successful goal attainment and this association was mediated by their tendency to select the goals that reflect their true/authentic self. These results were obtained using cross‐sectional and longitudinal designs and were robust against controlling for previously established mechanisms of the effect of trait self‐control on goal attainment (habit strength, experiences of goal‐conflicting desires). Overall, there findings contribute to the literatures on self‐regulation, authenticity and goal management.
Study on the mechanisms of refracturing technology featuring temporary plug for fracturing fluid diversion in tight sandstone reservoirs
Authors: Li Wei , Zhao Huan , Pu Hui , Zhang Yuguang , Wang Lei , Zhang Ligang , Sun Xiaofeng
Published: November 2018

Well production rates in unconventional plays usually decline dramatically in the first year. Refracturing, which is a remedial production operation, is often done because original hydraulic fracturing failed to contribute any significant amount of flow or significant unfractured pay exists in the well. In order to maximize the fracturing fluid contact with the intact rock and to stimulate more reservoir volume in previously stimulated wells, a refracturing technology featuring a novel temporary plugging for fluid diversion is developed to enable the fracturing fluid to reach the untouched areas and to create reoriented fractures. In this paper, laboratory physical simulation tests of refracturing using fiber for effective temporary plugging is carried out to study the refracture morphology and the influencing factors of refractures. Results show that the refracture morphology is affected by the horizontal stress difference, the injection rate of initial fracturing fluid, and the natural fractures. Under condition of the different horizontal stress differences, the fracture initiation and orientation angle are different. When the horizontal stress difference is small, it is easy to form large angle fractures. The injection rate of initial fracturing fluid affects the length of initial fractures and refractures. The smaller the initial fracturing fluid injection rate is, the better the effect of temporary plugging in refracturing. The presence of natural fractures will lead to reorientation of refractures to form a complex fracture network. This study provides a theoretical guidance and technology support for refracturing operations.
The use of CT scan imaging technique to determine pear bruise level due to external loads
Authors: Azadbakht Mohsen , Vahedi Torshizi Mohammad , Mahmoodi Mohammad Javad
Published: November 2018

X‐ray computed tomography (CT) is an effective noninvasive tool to visualize fresh agricultural commodities’ internal components and quality attributes, also imaging via X‐ray CT is a non‐destructive and well‐developed method applied in measuring the internal effects of agricultural products. In the present research, 120 healthy pears with their health verification through the CT were selected. Next, 81 healthy pears were selected and subjected to quasi‐static and dynamic loading. The impact of the incoming pressures was investigated within 5, 10, and 15 days of storage. After loading and storing with the use of CT method, the total volume and the bruise volume of the pears were measured and the ratio of the bruise volume to the volume of each pear was calculated. Quasi‐static loads were pressurized over a period of two ways; the pressure of wide edge was exerted at three force levels of 70, 100, and 130 N while the pressure of the thin edge was applied at 15, 20, and 25 N. Dynamic loading was performed by utilizing a pendulum and 300, 350, and 400 g mass. The results of the experiments indicated that in the quasi‐static loading, the maximum and minimum amounts of pear bruise were 45.138% and 0.094% of the fruit, respectively. Besides, in the case of thin edge pressures, the minimum and maximum bruise levels were 0.007% and 19.88%, respectively. These values were obtained through 5 and 15 days of storage, respectively. In the dynamic loading, the maximum and minimum amounts of pear bruise were 47.36% and 0.21% of the total fruit, respectively, occurring at 400 and 300 g mass impact.
Microbial identification of potato taste defect from coffee beans
Authors: Ndayambaje Jean Bernard , Nsabimana Antoine , Dushime Sylvie , Ishimwe Florence , Janvier Habinshuti , Ongol Martin P.
Published: November 2018

Coffee is a socioeconomic important plant all over the world due to its exportation and how it provides income to the farmers and the country. However, potato taste defect (PTD) affects the Rwandan coffee quality. The smell is reported to be caused by some bacteria that are responsible for the off‐flavor and may also be related to the infestation of Antestia pest which are in its elimination process. The aim of this study was to isolate, biochemically characterize, and identify bacteria producing potato flavor from Rwandan coffee. Five samples were obtained from different regions (Nyamasheke and Nyakizu) of Rwanda. Bacteria were isolated and enumerated in the nutrient agar media followed by culture on nutrient and tryptic soy broth media. Bacteria were also cultured to several carbon sources such as glucose, fructose, sucrose, starch, pectin, and galactose to smell the odor produced by those bacteria. DNA extraction of isolates was done, and the resulting DNA strands were undergone three steps of PCR to be amplified using the forward primer and reverse primer. The identification of bacteria producing potato flavor from Rwandan coffee beans was done through 16S rDNA method followed by sequence analysis using FinchTV software and BLAST. Earthy odor was the mostly produced one for nutrient agar and tryptic soy agar media, and for carbon sources such as sucrose, glucose, pectin, fructose, and galactose. The potato odor was recorded mostly from damaged floaters and hand‐sorted damaged coffee beans. However, other odors such as fruity and ferment were found to be produced by bacteria in coffee beans. The study came up by concluding the presence of different kinds of bacteria including Enterobacteriaceae and Pantoea, which are responsible for the formation of 2‐isopropyl‐3‐methoxypyrazine (IMP) in coffee beans and cause the production of potato flavor.