bluuebunny/crossref_metadata_2025 huggingface dataset:
Relation Between Severity of Coronary Artery Disease and Aorto-Ilio-Pudendal Artery Disease in Patients with Ischemic Heart Disease–Associated Vascular Erectile Dysfunction
Authors: Sanad Ahmed M. , Younis Soha E. , Oraby Mohamed A. , Hegazy Hesham , El-Sakka Ahmed I.
Published: March 2020

Background

The angiographically documented association between severity of coronary artery disease (CAD) and aorto-ilio-pudendal (A-I-P) artery disease and vascular erectile dysfunction (ED) was not yet settled.

Aim

To assess the relation between angiographically proved CAD and A-I-P artery disease in patients with ischemic heart disease (IHD)–associated vascular ED.

Methods

60 men were assigned to 3 study groups: Group 1 (n = 25), patients who had IHD and ED; group 2 (n = 25), patients who had IHD and had no ED; group 3 (n = 10), patients who had ED and had no suspected IHD. All patients were subjected to detailed medical, cardiac, and sexual history. International Index of Erectile Function and penile color Doppler ultrasound were used to assess ED. Quantitative coronary angiography and invasive angiography were used to assess the vascular tree for the right and left (A-I-P) arteries. Endothelial markers, that is, endothelial microparticles and endothelial progenitor cells were also assessed.

Outcomes

The main outcome measures are assessment of ED and angiographically proved CAD and A-I-P artery disease.

Results

The mean age ± SD of the 3 study groups were 50.4 ± 6.6, 51.4 ± 3.9, and 49.9 ± 6.1 years, respectively, with no statistically significant difference among groups (P = .380). There were significant higher rates of left main (LM) lesions (≥50%), CAD (≥70%), right and left internal pudendal artery lesions, and right and left internal iliac artery lesions in G1 in comparison with G2 and G3. Patients with ED alone had a higher rate of peripheral lesions compared with patients with CAD alone. 10 percent of patients with ED alone had CAD. Patients in G1 had notably higher rates of peripheral lesions than the other groups combined Patients with left internal pudendal artery lesions had a chance by 1.25 and 2.11 times to have LM lesions and significant CAD, respectively. There was a significant increase of endothelial microparticles in G1 in comparison with other groups (P < .05).

Clinical Implications

The clinical implications are uses of peripheral angiograghy as a diagnostic tool in patients with CAD-associated vascular ED may have a clinical merit.

Strengths & Limitations

The strengths in the present study are the use of angiography, color Doppler ultrasound, and standardized instruments. The main limitations are the small sample size and lack of intervention and longitudinal data.

Conclusion

ED correlates more with A-I-P vascular lesions compared with CAD alone. There was a statistically significant association between severity of CAD including LM significant lesions and A-I-P arteries disease in patients with CAD-associated vascular ED.
Improving Pediatric Administrative Disaster Preparedness Through Simulated Disaster Huddles
Authors: Gross Isabel T. , Goldberg Scott A. , Whitfill Travis , Liebling Storm , Garcia Angelica , Alfano April , Hasdianda Adrian , Cicero Mark X.
Published: March 2020

Members of an emergency department (ED) staff need to be prepared for mass casualty incidents (MCIs) at all times. Didactic sessions, drills, and functional exercises have shown to be effective, but it is challenging to find time and resources for appropriate training. We conducted brief, task-specific drills (deemed “disaster huddles”) in a pediatric ED (PED) to examine if such an approach could be an alternative or supplement to traditional MCI training paradigms. Over the course of the study, we observed an improving trend in the overall score for administrative disaster preparedness. Disaster huddles may be an effective way to improve administrative disaster preparedness in the PED. Low-effort, low-time commitment education could be an attractive way for further disaster preparedness efforts. Further studies are indicated to show a potential impact on lasting behavior and patient outcomes.
Ready or Not, Patients Will Present: Improving Urban Pandemic Preparedness
Authors: Madad Syra , Moskovitz Joshua , Boyce Matthew R , Cagliuso Nicholas V. , Katz Rebecca
Published: March 2020

Over the past century, society has achieved great gains in medicine, public health, and health-care infrastructure, particularly in the areas of vaccines, antibiotics, sanitation, intensive care and medical technology. Still, despite these developments, infectious diseases are emerging at unprecedented rates around the globe. Large urban centers are particularly vulnerable to communicable disease events, and must have well-prepared response systems, including on the front-line level. In November 2018, the United States’ largest municipal health-care delivery system, New York City Health + Hospitals, hosted a half-day executive-level pandemic response workshop, which sought to illustrate the complexity of preparing for, responding to, and recovering from modern-day infectious diseases impacting urban environments. Attendees were subjected to a condensed, plausible, pandemic influenza scenario and asked to simulate the high-level strategic decisions made by leaders by internal (eg, Chief Medical Officer, Chief Nursing Officer, and Legal Affairs) and external (eg, city, state, and federal public health and emergency management entities) partners across an integrated system of acute, postacute, and ambulatory sites, challenging players to question their assumptions about managing the consequences of a highly pathogenic pandemic.
The Threshold Problem, the Cluster Account, and the Significance of Knowledge
Authors: Immerman Daniel
Published: March 2020

The threshold problem is the task of adequately answering the question: “Where does the threshold lie between knowledge and lack thereof?” I start this paper by articulating two conditions for solving it. The first is that the threshold be neither too high nor too low; the second is that the threshold accommodate the significance of knowledge. In addition to explaining these conditions, I also argue that it is plausible that they can be met. Next, I argue that many popular accounts of knowledge cannot meet them. In particular, I lay out a number of problems that standard accounts of knowledge face in trying to meet these conditions. Finally, near the end of this paper, I argue that there is one sort of account that seems to evade these problems. This sort of account, which is called a cluster account of knowledge, says that knowledge is to be accounted for in terms of truth, belief and a cluster of epistemic properties and also that knowledge doesn't require having all members of the cluster, but merely some subset.
“A Country of Hair”: A Global Story of South Korean Wigs, Korean American Entrepreneurs, African American Hairstyles, and Cold War Industrialization
Authors: PETRULIS JASON
Published: March 2020

This article reinterprets Asian industrialization during the Cold War through the lens of a forgotten commodity: the South Korean wig. Wigs were critical to Asia’s “miraculous” economic growth—a US$1 billion industry in 1970, as well as the number two export in South Korea and number four in Hong Kong at the height of export-oriented industrialization. The article makes a methodological argument, suggesting that we see industrialization differently when we “follow” a commodity transnationally—from the heads of rural South Koreans to the hands of Seoul factory workers to the shoulder bags of Korean American peddlers to the heads of African American women—and when we integrate bottom-up and top-down views of the commodity’s “life.” Only by taking this global perspective can we see how U.S. imperialism shaped the ways people and things moved across borders and oceans and how Cold War commodities were haunted by the lives of the people who touched them.
Use of airborne infection isolation in potential cases of pulmonary tuberculosis
Authors: England James H. , Byrne Daniel W. , Harris Bryan D. , Talbot Thomas R.
Published: March 2020

Objective:

To identify risk factors of patients placed in airborne infection isolation (AII) for possible pulmonary tuberculosis (TB) to better predict TB diagnosis and allow more judicious use of AII.

Methods:

Case-control, retrospective study at a single tertiary-care academic medical center. The study included all adult patients admitted from October 1, 2014, through October 31, 2017, who were placed in AII for possible pulmonary TB. Cases were defined as those ultimately diagnosed with pulmonary TB. Controls were defined as those not diagnosed with pulmonary TB. Those with TB diagnosed prior to admission were excluded. In total, 662 admissions (558 patients) were included.

Results:

Overall, 15 cases of pulmonary TB were identified (2.7%); of these, 2 were people living with human immunodeficiency virus (HIV; PLWH). Statistical analysis was limited by low case number. Those diagnosed with pulmonary TB were more likely to have been born outside the United States (53% vs 13%; P < .001) and to have had prior positive TB testing, regardless of prior treatment (50% vs 19%; P = .015). A multivariate analysis using non–US birth and prior positive TB testing predicted an 18.2% probability of pulmonary TB diagnosis when present, compared with 1.0% if both factors were not present.

Conclusions:

The low number of pulmonary TB cases indicated AII overuse, especially in PLWH, and more judicious use of AII is warranted. High-risk groups, including those born outside the United States and those with prior positive TB testing, should be considered for AII in the appropriate clinical setting.
On four measures of taxonomic richness
Authors: Alroy John
Published: March 2020

The choice of measures used to estimate the richness of species, genera, or higher taxa is a crucial matter in paleobiology and ecology. This paper evaluates four methods called shareholder quorum subsampling, true richness estimated using a Poisson sampling model (TRiPS), squares, and the corrected first-order jackknife (cJ1). Quorum subsampling interpolates to produce a relative richness estimate, while the other three extrapolate to the size of the overall species pool. Here I use routine ecological data to show that squares and cJ1 pass several basic validation tests, but TRiPS does not. First, TRiPS estimates are insensitive to the shape of abundance distributions, being entirely predicted by total counts of species and of individuals regardless of the details. Furthermore, TRiPS tends not to extrapolate at all when sampling is moderate or intense. Second, all three extrapolators yield lower values when they work with small uniform subsamples of large raw inventories. The third test is a split-analyze-and-sum analysis: each inventory is divided between the most common and least common halves of the abundance distribution, the methods are applied to the half-inventories, and the estimates are summed. Squares and cJ1 perform well here, but TRiPS does not extrapolate as long as the full inventories are reasonably well-sampled. It is otherwise not particularly accurate. The extrapolators are largely insensitive to the influence of abundance distribution evenness, as quantified using Pielou's J and a new index called the ratio of means. Quorum subsampling generally performs well, but it stumbles on the split-analyze-and-sum test and is confounded somewhat by evenness.
The Matter of Ephemeral Art: Craft, Spectacle, and Power in Early Modern Europe
Authors: Smith Pamela H. , Uchacz Tianna Helena , Pitman Sophie , Taape Tillmann , Debuiche Colin
Published: March 2020

Through a close reading and reconstruction of technical recipes for ephemeral artworks in a manuscript compiled in Toulouse ca. 1580 (BnF MS Fr. 640), we question whether ephemeral art should be treated as a distinct category of art. The illusion and artifice underpinning ephemeral spectacles shared the aims and, frequently, the materials and techniques of art more generally. Our analysis of the manuscript also calls attention to other aspects of art making that reframe consideration of the ephemeral, such as intermediary processes, durability, the theatrical and transformative potential of materials, and the imitation and preservation of lifelikeness.
A short history of SLA: Where have we come from and where are we going?
Authors: Ellis Rod
Published: March 2020

If we want to understand where we are now, we need to consider where we have come from. This statement constitutes the strongest rationale for the study of history. It is relevant to any field of enquiry and it is certainly true of the field of second language acquisition (SLA). As Larsen-Freeman (2018) wrote in her own historical account of SLA ‘it is important to understand ideas at the time they originated’ (p. 56). I would add that it is also important to understand how the ideas that motivated a field of enquiry at one time evolved into and were sometimes replaced by ideas later on.
Tropical friezes and the index in higher homological algebra
Authors: JØRGENSEN PETER
Published: March 2020

Cluster categories and cluster algebras encode two dimensional structures. For instance, the Auslander–Reiten quiver of a cluster category can be drawn on a surface, and there is a class of cluster algebras determined by surfaces with marked points.

Cluster characters are maps from cluster categories (and more general triangulated categories) to cluster algebras. They have a tropical shadow in the form of so-called tropical friezes, which are maps from cluster categories (and more general triangulated categories) to the integers.

This paper will define higher dimensional tropical friezes. One of the motivations is the higher dimensional cluster categories of Oppermann and Thomas, which encode (d + 1)-dimensional structures for an integer d ⩾ 1. They are (d + 2)-angulated categories, which belong to the subject of higher homological algebra.

We will define higher dimensional tropical friezes as maps from higher cluster categories (and more general (d + 2)-angulated categories) to the integers. Following Palu, we will define a notion of (d + 2)-angulated index, establish some of its properties, and use it to construct higher dimensional tropical friezes.
Assessing the effects of different management scenarios on the conservation of small island vulture populations
Authors: ARESU MAURO , ROTTA ANDREA , FOZZI ALBERTO , CAMPUS ALFONSO , MUZZEDDU MARCO , SECCI DIONIGI , FOZZI ILARIA , DE ROSA DAVIDE , BERLINGUER FIAMMETTA
Published: March 2020

Although the population of Griffon VultureGyps fulvusis significantly increasing in Europe, in Italy the species is still on the Red List as ‘Critically Endangered’, with the last natural population persisting on the island of Sardinia. Several episodes of poisoning hampered the success of conservation actions implemented in the years 1987–1995. In 2005 there were estimated to be only 31–32 territorial pairs, with the population occupying the territories of Alghero and Bosa. We used a long-term dataset of reproductive records from the Sardinian Griffon Vulture populations to run a population viability analysis (PVA) to evaluate the extinction risk using the Vortex simulation software. The model estimated the probability of extinction over the next five generations (estimated generation time: 11 years, simulation time used: 55 years) as 96.4% for the Alghero population, and near-zero for the Bosa population. We used sensitivity analyses to understand how uncertainty about parameter values affect model outcomes. Population projections were evaluated under different management scenarios tackling the main threats (poisoning and human disturbance) and implementing conservation actions (supplementary feeding and restocking). Our results showed that population size is a critical factor in affecting the projections of population dynamics of Griffon Vultures. Sensitivity analyses highlighted the importance of poisoning events to population persistence and showed that juvenile and adult mortality rates had a secondary impact on population viability. The only conservation measure effective in significantly increasing stochastic growth rates in the Alghero population, whose initial population was set at five individuals, was the complete removal of poisoning events. When targeting the Bosa population (initial population size 94 individuals), supplementary feeding, mitigation of the risk of poisoning episodes, restocking, and mitigation of human disturbance in the reproductive sites significantly increased stochastic growth rate. A cost-effectiveness analysis should be performed to prioritise interventions.
Witness algebra and anyon braiding
Authors: Blass Andreas , Gurevich Yuri
Published: March 2020

Topological quantum computation employs two-dimensional quasiparticles called anyons. The generally accepted mathematical basis for the theory of anyons is the framework of modular tensor categories. That framework involves a substantial amount of category theory and is, as a result, considered rather difficult to understand. Is the complexity of the present framework necessary? The computations of associativity and braiding matrices can be based on a much simpler framework, which looks less like category theory and more like familiar algebra. We introduce that framework here.
The ‘nouniness’ of attributive adjectives and ‘verbiness’ of predicative adjectives: evidence from phonology
Authors: HOLLMANN WILLEM B.
Published: March 2020

This article investigates prototypically attributive versus predicative adjectives in English in terms of the phonological properties that have been associated especially with nouns versus verbs in a substantial body of psycholinguistic research (e.g. Kelly 1992) – often ignored in theoretical linguistic work on word classes. Inspired by Berg's (2000, 2009) ‘cross-level harmony constraint’, the hypothesis I test is that prototypically attributive adjectives not only align more with nouns than with verbs syntactically, semantically and pragmatically, but also phonologically – and likewise for prototypically predicative adjectives and verbs. I analyse the phonological structure of frequent adjectives from theCorpus of Contemporary American English(COCA), and show that the data do indeed support the hypothesis. Berg's ‘cross-level harmony constraint’ may thus apply not only to the entire word classes noun, verb and adjective, but also to these two adjectival subclasses. I discuss several theoretical issues that emerge. The facts are most readily accommodated in a usage-based model, such as Radical Construction Grammar (Croft 2001), where these adjectives are seen as forming two distinct but overlapping classes. Drawing also on recent research by Boyd & Goldberg (2011) and Hao (2015), I explore the possible nature and emergence of these classes in some detail.
Is the proton–boron fusion therapy effective?
Authors: Khaledi Navid , Wang Xufei , Hosseinabadi Roghiye B. , Samiei Farhad
Published: March 2020

Introduction:

In the recent years, some publications (mainly from one group of authors) have dealt with the effectiveness of proton–boron fusion therapy (PBFT). This theory is based on the Q-value of three produced α particles in the reaction of protons with boron (11B). They claim that this reaction significantly increases the absorbed dose in the target volume. However, the current study would re-evaluate their method to show if PBFT is really effective.

Methods and materials:

A parallel 80-MeV proton beam was irradiated on a water medium in a cubic boron uptake region (BUR). The two-dimensional dose distribution and percentage depth dose of protons, alphas and all particles were calculated using tally F6 and mesh-tallies by Monte Carlo N Particle Transport code.

Results:

The results not only showed that the dose enhancement in BUR is neglectable but also the higher density of BUR in comparison with water led to decrement of dose in this region. Because of low cross section of boron for proton beam (<100 mb), the α particles’ dose is 1,000 times lower than the proton dose.

Conclusions:

The physical aspects and the simulation results did not show any effectiveness of the PBFT for proton therapy dose enhancement.
Trust in the Law? Mutual Recognition as a Justification to Domestic Criminal Procedure
Authors: Öberg Jacob
Published: March 2020

EU law – Mutual recognition as a justification for EU competence – EU competence in domestic criminal procedure – Test and intensity of judicial review of EU criminal law legislation – Relationship between mutual trust and mutual recognition – Federalism and division of powers between the member states and the EU – Mutual recognition as a constraint to EU action – Presumption of Innocence Directive – Victims’ Rights Directive – Exercise of EU competence in domestic criminal procedure – Justifications for EU action in criminal procedure – Evidence-based legislation in the field of criminal procedure – Mutual trust as a legal and sociological concept – National courts’ compliance with EU law – European arrest warrant
National Parliaments’ Scrutiny of the Principle of Subsidiarity: Reasoned Opinions 2014–2019
Authors: Jaroszyński Tomasz
Published: March 2020

Principle of subsidiarity – Early Warning Mechanism – Protocol No. 2 on Proportionality and Subsidiarity – The scope of the principle of subsidiarity – The role of national parliaments in the EU – National parliaments’ reasoned opinions – Principle of proportionality – Principle of conferral – National sovereignty in the EU – National identity in the EU – Justification of draft legislation acts
Direct Determination of a Bare Neutron Counter Yield Function
Authors: Nuntiyakul W. , Mangeard P.‐S. , Ruffolo D. , Evenson P. , Bieber J. W. , Clem J. , Hallgren A. , Madsen J. , Pyle R. , Sáiz A. , Tilav S.
Published: March 2020

Ground‐based neutron counters are a standard tool for detecting atmospheric showers from GeV range primary cosmic rays of either solar or galactic origin. Bare neutron counters, a type of lead‐free neutron monitor, function much like standard neutron monitors but have different yield functions primarily because they are more sensitive to neutrons of lower energy. When operated together with standard monitors, the different yield functions allow estimates to be made of the energy spectrum of galactic or solar particles. In 2010 a new array of 12 bare neutron detectors was installed at the South Pole to operate together with the neutron monitor there. Prior to installation, two of the detectors were operated on a ship that traveled from Sweden to Antarctica and back from November 2009 to April 2010. The purpose of this latitude survey was to use Earth's magnetic field as a spectrometer, blocking cosmic rays below the local cutoff rigidity (momentum per unit charge), from which we determined the response function versus rigidity of these bare counters. By comparing that measured response function to direct measurements of the cosmic ray spectrum taken by the PAMELA spacecraft, we were able to make a direct determination of the yield function for these detectors.
The Influence of Carbonate Platforms on the Geomorphological Development of a Mixed Carbonate‐Siliciclastic Margin (Great Barrier Reef, Australia)
Authors: Thran Amanda C. , East Madison , Webster Jody M. , Salles Tristan , Petit Carole
Published: March 2020

Sedimentation regimes on the Great Barrier Reef margin often do not conform to more conventional sequence stratigraphic models, presenting difficulties when attempting to identify key processes that control the margin's geomorphological evolution. By obstructing and modifying down‐shelf and down‐slope flows, carbonate platforms are thought to play a central role in altering the distribution and morphological presentation of common margin features. Using numerical simulations, we test the role of the carbonate platforms in reproducing several features (i.e., paleochannels, shelf‐confined fluvial sediment mounds, shelf‐edge deltas, canyons, and surface gravity flows) that have been described from observational data (seismic sections, multibeam bathymetry, sediment cores, and backscatter imagery). When carbonate platforms are present in model simulations, several notable geomorphological features appear, especially during lowstand. Upon exposure of the shelf, platforms reduce stream power, promoting mounding of fluvial sediments around platforms. On the outer shelf, rivers and streams are re‐routed and coalesce between platforms, depositing shelf‐edge deltas and incising paleochannels through knickpoint retreat. Additionally, steep platform topography triggers incision of slope canyons through turbidity currents, and platforms act as conduits for the localized delivery of land and shelf‐derived sediments to the continental slope and basin. When platforms are absent from the topographic surface, the model is unable to reproduce many of these features. Instead, a more typical “reciprocal‐type” sedimentation regime arises. Our results demonstrate the essential role of carbonate platform topography in modulating key bedload processes. Therefore, they exert direct control on the development of various geomorphological features within the shelf, slope, and basin environments.
Network-based drug repurposing for novel coronavirus 2019-nCoV/SARS-CoV-2
Authors: Zhou Yadi , Hou Yuan , Shen Jiayu , Huang Yin , Martin William , Cheng Feixiong
Published: March 2020

Human coronaviruses (HCoVs), including severe acute respiratory syndrome coronavirus (SARS-CoV) and 2019 novel coronavirus (2019-nCoV, also known as SARS-CoV-2), lead global epidemics with high morbidity and mortality. However, there are currently no effective drugs targeting 2019-nCoV/SARS-CoV-2. Drug repurposing, representing as an effective drug discovery strategy from existing drugs, could shorten the time and reduce the cost compared to de novo drug discovery. In this study, we present an integrative, antiviral drug repurposing methodology implementing a systems pharmacology-based network medicine platform, quantifying the interplay between the HCoV–host interactome and drug targets in the human protein–protein interaction network. Phylogenetic analyses of 15 HCoV whole genomes reveal that 2019-nCoV/SARS-CoV-2 shares the highest nucleotide sequence identity with SARS-CoV (79.7%). Specifically, the envelope and nucleocapsid proteins of 2019-nCoV/SARS-CoV-2 are two evolutionarily conserved regions, having the sequence identities of 96% and 89.6%, respectively, compared to SARS-CoV. Using network proximity analyses of drug targets and HCoV–host interactions in the human interactome, we prioritize 16 potential anti-HCoV repurposable drugs (e.g., melatonin, mercaptopurine, and sirolimus) that are further validated by enrichment analyses of drug-gene signatures and HCoV-induced transcriptomics data in human cell lines. We further identify three potential drug combinations (e.g., sirolimus plus dactinomycin, mercaptopurine plus melatonin, and toremifene plus emodin) captured by the “Complementary Exposure” pattern: the targets of the drugs both hit the HCoV–host subnetwork, but target separate neighborhoods in the human interactome network. In summary, this study offers powerful network-based methodologies for rapid identification of candidate repurposable drugs and potential drug combinations targeting 2019-nCoV/SARS-CoV-2.
Framework for atomic-level characterisation of quantum computer arrays by machine learning
Authors: Usman Muhammad , Wong Yi Zheng , Hill Charles D. , Hollenberg Lloyd C. L.
Published: March 2020

Atomic-level qubits in silicon are attractive candidates for large-scale quantum computing; however, their quantum properties and controllability are sensitive to details such as the number of donor atoms comprising a qubit and their precise location. This work combines machine learning techniques with million-atom simulations of scanning tunnelling microscopic (STM) images of dopants to formulate a theoretical framework capable of determining the number of dopants at a particular qubit location and their positions with exact lattice site precision. A convolutional neural network (CNN) was trained on 100,000 simulated STM images, acquiring a characterisation fidelity (number and absolute donor positions) of >98% over a set of 17,600 test images including planar and blurring noise commensurate with experimental measurements. The formalism is based on a systematic symmetry analysis and feature-detection processing of the STM images to optimise the computational efficiency. The technique is demonstrated for qubits formed by single and pairs of closely spaced donor atoms, with the potential to generalise it for larger donor clusters. The method established here will enable a high-precision post-fabrication characterisation of dopant qubits in silicon, with high-throughput potentially alleviating the requirements on the level of resources required for quantum-based characterisation, which will otherwise be a challenge in the context of large qubit arrays for universal quantum computing.
MHD surrogate model for convection in electromagnetically levitated molten metal droplets processed using the ISS-EML facility
Authors: Baker Evan B. , Nawer Jannatun , Xiao Xiao , Matson Douglas M.
Published: March 2020

Electromagnetic levitation experiments in space are an essential tool for thermophysical property measurement and solidification studies. In light of the need for material properties as inputs to industrial process modeling, investigators need new tools for efficient experiment planning. MHD surrogate modeling is a parametric method for prediction of flow conditions during processing using the ISS-EML facility. Flow conditions in model Au, Zr, and Ti39.5Zr39.5Ni21 samples are predicted using the surrogate model. For Au, flow is shown be turbulent in nearly all experimental conditions, making property measurement difficult. For Zr, the flow is turbulent with the heater on and laminar with the heater off, allowing for property measurement during free-cooling experiments only. For TiZrNi, the flow is laminar under all experimental conditions, indicating that TiZrNi is an excellent candidate for EML experiments. This surrogate modeling approach can be easily applied to other materials of interest, enabling investigators to choose materials that will perform well in levitation and to tailor experiment parameters to achieve desirable flow conditions.
Artificial Intelligence based Models for Screening of Hematologic Malignancies using Cell Population Data
Authors: Syed-Abdul Shabbir , Firdani Rianda-Putra , Chung Hee-Jung , Uddin Mohy , Hur Mina , Park Jae Hyeon , Kim Hyung Woo , Gradišek Anton , Dovgan Erik
Published: March 2020

Cell Population Data (CPD) provides various blood cell parameters that can be used for differential diagnosis. Data analytics using Machine Learning (ML) have been playing a pivotal role in revolutionizing medical diagnostics. This research presents a novel approach of using ML algorithms for screening hematologic malignancies using CPD. The data collection was done at Konkuk University Medical Center, Seoul. A total of (882 cases: 457 hematologic malignancy and 425 hematologic non-malignancy) were used for analysis. In our study, seven machine learning models, i.e., SGD, SVM, RF, DT, Linear model, Logistic regression, and ANN, were used. In order to measure the performance of our ML models, stratified 10-fold cross validation was performed, and metrics, such as accuracy, precision, recall, and AUC were used. We observed outstanding performance by the ANN model as compared to other ML models. The diagnostic ability of ANN achieved the highest accuracy, precision, recall, and AUC ± Standard Deviation as follows: 82.8%, 82.8%, 84.9%, and 93.5% ± 2.6 respectively. ANN algorithm based on CPD appeared to be an efficient aid for clinical laboratory screening of hematologic malignancies. Our results encourage further work of applying ML to wider field of clinical practice.
Patterns of genetic diversity in North Africa: Moroccan-Algerian genetic split in Juniperus thurifera subsp. africana
Authors: Taib Asma , Morsli Abdelkader , Chojnacka Aleksandra , Walas Łukasz , Sękiewicz Katarzyna , Boratyński Adam , Romo Àngel , Dering Monika
Published: March 2020

Juniperus thurifera is a key element of the forest communities in arid and semi-arid areas of the western Mediterranean. Previous genetic and morphological investigations suggested that Algerian populations are genetically more similar to European than to Moroccan populations and advocated their recognition at the variety rank. We aimed to investigate the spatial genetic structure in J. thurifera to verify the distinct character of the Algerian population in terms of the genetic breaks reported among several North African taxa. We also modelled species distributions since the Eemian to recognise the impact of past climatic changes on the current pattern of diversity and predict possible changes in species distribution in the future. Species-specific microsatellites were used in the analysis of 11 populations from Algeria, Morocco and Europe. We revealed the significant genetic distinctiveness of the Algerian populations from the Moroccan and European stands that may have important taxonomic and conservation implications. The diversity pattern revealed for J. thurifera reflects the east-west genetic splits reported among some North African plant and animal taxa and suggests an impact of shared historical processes. Additionally, modelling of the distribution allowed us to identify possible glacial refugia and their impact on the modern pattern of differentiation in J. thurifera. Reduction of species occurrence, especially in the European domain, is likely according to the future projections of the species distribution.
Earliest African evidence of carcass processing and consumption in cave at 700 ka, Casablanca, Morocco
Authors: Daujeard Camille , Falguères Christophe , Shao Qingfeng , Geraads Denis , Hublin Jean-Jacques , Lefèvre David , Graoui Mohssine El , Rué Mathieu , Gallotti Rosalia , Delvigne Vincent , Queffelec Alain , Arous Eslem Ben , Tombret Olivier , Mohib Abderrahim , Raynal Jean-Paul
Published: March 2020

To date, in Africa, evidence for animal processing and consumption in caves routinely used as living spaces is only documented in the late Middle Pleistocene of the North and South of the continent and postdates the Middle Pleistocene in East Africa. Here we report the earliest evidence in a North-African cave (Grotte des Rhinocéros at Casablanca, Morocco) of cut, percussion and human gnawing marks on faunal remains directly associated with lithic knapping activities in the same space and in a well-documented stratified context. Ages for this Acheulean site are provided by the dating of herbivorous teeth to 690–720 ka and 520–550 ka (lower and upper sets) by combined Electron Spin Resonance (ESR) and U-series techniques. Traces of butchery on gazelle, alcelaphin, and zebra bones demonstrate that hominins had primary access to herbivore carcasses. Hominins brought and consumed meat in the cave, as documented by herbivore bones bearing human tooth marks concentrated in a circumscribed area of the excavation. In Africa, this site provides the earliest evidence forin situcarcass processing and meat-eating in cave, directly associated with lithic production and demonstrates the recurrent use by early Middle Pleistocene hominins of a North African cave site 400 000 years before that byHomo sapiensat Jebel Irhoud (Morocco).
Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance
Authors: Ouso Daniel O. , Otiende Moses Y. , Jeneby Maamun M. , Oundo Joseph W. , Bargul Joel L. , Miller Scott E. , Wambua Lillian , Villinger Jandouwe
Published: March 2020

Reliable molecular identification of vertebrate species from morphologically unidentifiable tissue is critical for the prosecution of illegally-traded wildlife products, conservation-based biodiversity research, and identification of blood-meal hosts of hematophagous invertebrates. However, forensic identification of vertebrate tissue relies on sequencing of the mitochondrial cytochrome oxidase I (COI) ‘barcode’ gene, which remains costly for purposes of screening large numbers of unknown samples during routine surveillance. Here, we adapted a rapid, low-cost approach to differentiate 10 domestic and 24 wildlife species that are common in the East African illegal wildlife products trade based on their unique high-resolution melting profiles from COI, cytochrome b, and 16S ribosomal RNA gene PCR products. Using the approach, we identified (i) giraffe among covertly sampled meat from Kenyan butcheries, and (ii) forest elephant mitochondrial sequences among savannah elephant reference samples. This approach is being adopted for high-throughput pre-screening of potential bushmeat samples in East African forensic science pipelines.
Self-regulation versus social influence for promoting cooperation on networks
Authors: Madeo Dario , Mocenni Chiara
Published: March 2020

Cooperation is a relevant and controversial phenomenon in human societies. Indeed, although it is widely recognized essential for tackling social dilemmas, finding suitable policies for promoting cooperation can be arduous and expensive. More often, it is driven by pre-established schemas based on norms and punishments. To overcome this paradigm, we highlight the interplay between the influence of social interactions on networks and spontaneous self-regulating mechanisms on individuals behavior. We show that the presence of these mechanisms in a prisoner’s dilemma game, may oppose the willingness of individuals to defect, thus allowing them to behave cooperatively, while interacting with others and taking conflicting decisions over time. These results are obtained by extending the Evolutionary Game Equations over Networks to account for self-regulating mechanisms. Specifically, we prove that players may partially or fully cooperate whether self-regulating mechanisms are sufficiently stronger than social pressure. The proposed model can explain unconditional cooperation (strong self-regulation) and unconditional defection (weak self-regulation). For intermediate self-regulation values, more complex behaviors are observed, such as mutual defection, recruiting (cooperate if others cooperate), exploitation of cooperators (defect if others cooperate) and altruism (cooperate if others defect). These phenomena result from dynamical transitions among different game structures, according to changes of system parameters and cooperation of neighboring players. Interestingly, we show that the topology of the network of connections among players is crucial when self-regulation, and the associated costs, are reasonably low. In particular, a population organized on a random network with a Scale-Free distribution of connections is more cooperative than on a network with an Erdös-Rényi distribution, and, in turn, with a regular one. These results highlight that social diversity, encoded within heterogeneous networks, is more effective for promoting cooperation.
Use of mouse-tracking software to detect faking-good behavior on personality questionnaires: an explorative study
Authors: Mazza Cristina , Monaro Merylin , Burla Franco , Colasanti Marco , Orrù Graziella , Ferracuti Stefano , Roma Paolo
Published: March 2020

The aim of the present study was to explore whether kinematic indicators could improve the detection of subjects demonstrating faking-good behaviour when responding to personality questionnaires. One hundred and twenty volunteers were randomly assigned to one of four experimental groups (honest unspeeded, faking-good unspeeded, honest speeded, and faking-good speeded). Participants were asked to respond to the MMPI-2 underreporting scales (L, K, S) and the PPI-R Virtuous Responding (VR) scale using a computer mouse. The collected data included T-point scores on the L, K, S, and VR scales; response times on these scales; and several temporal and spatial mouse parameters. These data were used to investigate the presence of significant differences between the two manipulated variables (honest vs. faking-good; speeded vs. unspeeded). The results demonstrated that T-scores were significantly higher in the faking-good condition relative to the honest condition; however, faking-good and honest respondents showed no statistically significant differences between the speeded and unspeeded conditions. Concerning temporal and spatial kinematic parameters, we observed mixed results for different scales and further investigations are required. The most consistent finding, albeit with small observed effects, regards the L scale, in which faking-good respondents took longer to respond to stimuli and outlined wider mouse trajectories to arrive at the given response.
Transcriptomic identification of miR-205 target genes potentially involved in metastasis and survival of cutaneous malignant melanoma
Authors: Sánchez-Sendra Beatriz , Serna Eva , Navarro Lara , González-Muñoz Jose F. , Portero Jesica , Ramos Alberto , Murgui Amelia , Monteagudo Carlos
Published: March 2020

Cutaneous melanoma is an aggressive neoplasm and is responsible for the majority of skin cancer deaths. Several miRNAs are involved in melanoma tumor progression. One of them is miR-205, the loss of which contributes to the development of melanoma metastasis. We evaluated whole-genome mRNA expression profiling associated with different miR-205 expression levels in melanoma cells. Differential expression analysis identified 243 differentially expressed transcripts including inositol polyphosphate 5′-phosphatase-like protein-1 (INPPL1) and BTB/POZ Domain-Containing Protein 3 (BTBD3). INPPL1 and BTBD3 were downregulated when melanoma cells expressed miR-205, indicating that these genes are potential miR-205 targets. Additionally, the target prediction algorithm TargetScan revealed that INPPL1 and BTBD3 genes had predicted target sites of miR-205 in their 3′UTRs and functional analysis demonstrated that these genes were directly linked to miR-205. Interestingly, our clinical data showed that INPPL1 was significantly associated with lymph node metastasis-free survival (LNMFS), distant metastasis-free survival (DMFS) and melanoma specific survival (MSS). This study supports INPPL1 as a miR-205 target gene and, therefore, that the involvement of miR-205 in the metastatic dissemination of malignant melanoma is, at least in part, via INPPL1.
Rhythmic expression of the melatonergic biosynthetic pathway and its differential modulation in vitro by LPS and IL10 in bone marrow and spleen
Authors: Córdoba-Moreno Marlina O. , de Souza Ewerton da Silva , Quiles Caroline L. , dos Santos-Silva Débora , Kinker Gabriela S. , Muxel Sandra M. , Markus Regina P. , Fernandes Pedro A.
Published: March 2020

Daily oscillation of the immune system follows the central biological clock outputs control such as melatonin produced by the pineal gland. Despite the literature showing that melatonin is also synthesized by macrophages and T lymphocytes, no information is available regarding the temporal profile of the melatonergic system of immune cells and organs in steady-state. Here, the expression of the enzymes arylalkylamine-N-acetyltransferase (AA-NAT), its phosphorylated form (P-AA-NAT) and acetylserotonin-O-methyltransferase (ASMT) were evaluated in phagocytes and T cells of the bone marrow (BM) and spleen. We also determined how the melatonergic system of these cells is modulated by LPS and the cytokine IL-10. The expression of the melatonergic enzymes showed daily rhythms in BM and spleen cells. Melatonin rhythm in the BM, but not in the spleen, follows P-AA-NAT daily variation. In BM cells, LPS and IL10 induced an increase in melatonin levels associated with the increased expressions of P-AA-NAT and ASMT. In spleen cells, LPS induced an increase in the expression of P-AA-NAT but not of melatonin. Conversely, IL10 induced a significant increase in melatonin production associated with increased AA-NAT/P-AA-NAT expressions. In conclusion, BM and spleen cells present different profiles of circadian production of local melatonin and responses to immune signals.
Tethered primary hepatocyte spheroids on polystyrene multi-well plates for high-throughput drug safety testing
Authors: Tasnim Farah , Singh Nisha Hari , Tan Elijah Keng Foo , Xing Jiangwa , Li Huan , Hissette Sebastien , Manesh Sravanthy , Fulwood Justina , Gupta Kapish , Ng Chan Way , Xu Shuoyu , Hill Jeffrey , Yu Hanry
Published: March 2020

Hepatocyte spheroids are useful models for mimicking liver phenotypes in vitro because of their three-dimensionality. However, the lack of a biomaterial platform which allows the facile manipulation of spheroid cultures on a large scale severely limits their application in automated high-throughput drug safety testing. In addition, there is not yet a robust way of controlling spheroid size, homogeneity and integrity during extended culture. This work addresses these bottlenecks to the automation of hepatocyte spheroid culture by tethering 3D hepatocyte spheroids directly onto surface-modified polystyrene (PS) multi-well plates. However, polystyrene surfaces are inert toward functionalization, and this makes the uniform conjugation of bioactive ligands very challenging. Surface modification of polystyrene well plates is achieved herein using a three-step sequence, resulting in a homogeneous distribution of bioactive RGD and galactose ligands required for spheroid tethering and formation. Importantly, treatment of polystyrene tethered spheroids with vehicle and paradigm hepatotoxicant (chlorpromazine) treatment using an automated liquid handling platform shows low signal deviation, intact 3D spheroidal morphology and Z’ values above 0.5, and hence confirming their amenability to high-throughput automation. Functional analyses performance (i.e. urea and albumin production, cytochrome P450 activity and induction studies) of the polystyrene tethered spheroids reveal significant improvements over hepatocytes cultured as collagen monolayers. This is the first demonstration of automated hepatotoxicant treatment on functional 3D hepatocyte spheroids tethered directly on polystyrene multi-well plates, and will serve as an important advancement in the application of 3D tethered spheroid models to high throughput drug screening.
Prevalence and new onset of depression and anxiety among participants with AMD in a European cohort
Authors: Rezapour Jasmin , Schuster Alexander K. , Nickels Stefan , Korb Christina A. , Elbaz Hisham , Peto Tunde , Michal Matthias , Münzel Thomas , Wild Philipp S. , König Jochem , Lackner Karl , Schulz Andreas , Pfeiffer Norbert , Beutel Manfred E.
Published: March 2020

To investigate the prevalence and new onset of depression and anxiety among subjects with age-related macular degeneration (AMD) and its association with AMD in a large European cohort with relatively good visual acuity. 11,834 participants enrolled in the German population-based Gutenberg Health Study were studied. AMD was diagnosed by grading of fundus photographs. Depression and anxiety were assessed with the Patient Health Questionnaire and the Generalized Anxiety Disorder-2 Scale, respectively. Logistic regression analyses were performed and adjusted for several parameters. 1,089 (9.2%) participants were diagnosed having AMD. Prevalence of depression in AMD and non-AMD participants was 7.2% and 8.0%, respectively and prevalence of anxiety was 4.2% and 7.0%, respectively. New onset of depression and anxiety at 5-year follow-up in AMD subjects was 2.6% and 3.6%, respectively. AMD was not associated with depression (OR 0.93; CI 95% 0.70–1.20; p = 0.62). AMD was associated with less anxiety (OR 0.67; CI 95% 0.47–0.93; p = 0.02). This is the first study analyzing both prevalence and new onset of depression and anxiety in AMD subjects. AMD- and non-AMD participants had a similar prevalence and new onset of depression in our population-based sample. Participants without AMD had a higher prevalence of anxiety. AMD was not associated with depression.