bluuebunny/crossref_metadata_2025 huggingface dataset:
The P4‐ATPase Drs2 interacts with and stabilizes the multisubunit tethering complex TRAPPIII in yeast
Authors: Pazos Irene , Puig‐Tintó Marta , Betancur Laura , Cordero Jorge , Jiménez‐Menéndez Nereida , Abella Marc , Hernández Altair C , Duran Ana G , Adachi‐Fernández Emi , Belmonte‐Mateos Carla , Sabido‐Bozo Susana , Tosi Sébastien , Nezu Akiko , Oliva Baldomero , Colombelli Julien , Graham Todd R , Yoshimori Tamotsu , Muñiz Manuel , Hamasaki Maho , Gallego Oriol
Published: March 2023

Multisubunit Tethering Complexes (MTCs) are a set of conserved protein complexes that tether vesicles at the acceptor membrane. Interactions with other components of the trafficking machinery regulate MTCs through mechanisms that are partially understood. Here, we systematically investigate the interactome that regulates MTCs. We report that P4‐ATPases, a family of lipid flippases, interact with MTCs that participate in the anterograde and retrograde transport at the Golgi, such as TRAPPIII. We use the P4‐ATPase Drs2 as a paradigm to investigate the mechanism and biological relevance of this interplay during transport of Atg9 vesicles. Binding of Trs85, the sole‐specific subunit of TRAPPIII, to the N‐terminal tail of Drs2 stabilizes TRAPPIII on membranes loaded with Atg9 and is required for Atg9 delivery during selective autophagy, a role that is independent of P4‐ATPase canonical functions. This mechanism requires a conserved I(S/R)TTK motif that also mediates the interaction of the P4‐ATPases Dnf1 and Dnf2 with MTCs, suggesting a broader role of P4‐ATPases in MTC regulation.
Role of multiplex PCR in the management of acute infectious diarrhea in a hospital setting – a single center retrospective study
Authors: Stephan Hellmig Stephan Hellmig , Schindewolf Kai , Bode Andre
Published: March 2023

Background and Aim Isolation measures in patients with acute diarrhea produce enormous costs of materials and personnel. Aim of this retrospective study was to evalute the percentage of unnecessary isolations and the impact of multiplex PCR stool testing on isolation days before and after introduction into clinical practice. Methods: Between July 2020 and July 2022 in total 2373 stool specimens of patients with acute diarrhea and a course no longer than 14 days were analyzed using BioFire® FilmArray® Gastrointestinal (GI) Panel. Number of isolation beds per day were compared before and after the introduction of Multiplex PCR testing. Results: In 65% of all specimen examined no pathogen was detected. Single-room isolation was a necessary in only 22% of all cases. Isolation days on the gastroenterological ward decreased from 635 in 2019 to 384 in 2020, a reduction of 39,5%. Conclusion: In acute infectious diarrhea Multiplex PCR is a use full tool to rapidly identify the causative agent and exclude the necessity of isolation in the majority of cases. In spite of all medical and economical advantages Multiplex PCR testing has not been established widely so far.
Case study review: pharmacognosy of complementary and alternative medicines as effective adjuncts in the treatment of mild to moderate hypertension
Authors: Tulp Orien L
Published: March 2023

The applications of Complementary and Alternative medicine strategies using naturally occurring plants and plant products have played an important role in the treatment and amelioration of illness and disease for hundreds if not thousands of years in many parts of the world. The practice of Traditional Chinese Medicine (TCM) includes an extensive nutraceutical formulary with its origins dating back for over 2,000 years, and in North American and other indigenous societies the use of locally available naturally occurring plants contributed to the prevention of numerous nutritional deficiencies including scurvy. In European cultures, and in Central and South American societies the use of natural products also contributed an important role in their health and wellbeing. In the Caribbean, the earliest populations arose from migrating from indigenous peoples from South and Central America with later immigrants arriving from Europe and the African continent, bringing with them the plants and animals that were important to their respective cultures to add to the local environments. This resulted in the abundance of nutraceutical related plants throughout the region, and that the Caribbean now hosts one of the most diverse and extensive arrays of medicinal plants known to exist on the planet. In the present editorial we will review the Pharmacognosy and use of TCM and CAM strategies with practices originating in China and the Caribbean region respectively as compared to allopathic medications on the treatment of mild to moderate intensity hypertension.
Review of issues on knowledge management system and country’s digital security: a study of Nigeria
Authors: Adejumo Dauda Adegoke
Published: March 2023

The digital world has exposed the whole world into danger and vulnerability of information security and the presence of new ways of compromising information security has moved the attention to a more holistic approach to information security management comprising technological, organizational, and social components. This remains a challenge for the policy maker and practitioners to more concerted effort into creating secure knowledge management and making the appropriate knowledge accessible to the appropriate individuals at the appropriate time. Knowledge is known as a country intellectual infrastructural and significant to overall development of all sectors.
Patterns of anabolic-androgenic steroid use, aesthetic doping, and body imagewithin the male Brazilian bodybuilding culture: an ethnographic approach
Authors: Moura Santos Azenildo , Silva Lippo Bruno Rodrigoda , Bahrke Michael S. , Yonamine Mauricio
Published: March 2023

The image of overly muscular bodies may appear to those not familiar with thise bodybuilding culture, that muscle is the symbol of masculinity. Much of the time, however, the pursuit of the ideal body, the result of focused discipline to reach the ultimate muscular shape, is often confounded by the concomitant use of anabolic-androgenic steroids (AAS). The purpose of the present study was to investigate how ergogenic aids, in particular AAS, are included within the culture of bodybuilding in relationship to aesthetics, body image, and health risks. Using qualitative data, generated during an ethnographic investigation of the male bodybuilding culture in the city of Recife, Brazil. Including interviews, documentation of events, and field notes. The interviewees consisted of 11 males from the three gyms. In addition, narcissism, and the pattern of AAS use are noted, since they play a significant role in the bodybuilding culture.
Milroy syndrome: apropos of a clinical case
Authors: Gamboa Sebastiana , dos Anjos Victoriano , Van-Dúnem Joaquim , Cardoso Ondina
Published: March 2023

Because Milroy syndrome is a rare disease, it aroused interest among pediatricians at the David Bernardino Pediatric Hospital in Luanda and for this reason we present this clinical case.
Карел Гавличек Боровский и Россия
Authors: Příhoda Marek
Published: March 2023

The aim of this article is to provide a systematic research of the views on Russia of the prominent Czech journalist, writer and politician Karel Havlicek Borovsky (1821–1856). Since the perception of Russia and Russians in the then Czech lands is inextricably linked to the Slavic idea (Slavism), attention is also paid to Havlicek’s reflections on the Slavic question, his opinions about other Slavs and, last but not least, his Czech national program.
Obraz historie vlasti v českých a polských učebnicích
Authors: Zormanová Michaela
Published: March 2023

This article aims to compare the presentation of national history in Czech and Polish literature textbooks for pupils from the first grade to the end of secondary school. Looking at history as a phenomenon will help to better understand the presentation of specific historical events. Comparisons will be made using discursive analysis.
De-Radicalization of Former Terrorists
Authors: Kato Hisanori
Published: March 2023

This article depicts the reality of de-radicalization programmes for former terrorists in Indonesia. The article aims to clarify the central advocates for, and content of, the programmes. There are several institutions that are engaged in de-radicalizing former terrorists, including both government and non-government organizations. The government is represented by the National Counter Terrorism Agency or Badan Nasional Penanggalangan Terorism (BNPT). This article also examines how these institutions play a role in the process of de-radicalization. The actual voices of former terrorists obtained through fieldwork will also be analysed with regard to how they came to regret their actions in order to scrutinize the process of their religious metamorphosis. The concept of forgiveness in Islam will also be discussed. As some might believe that Islam is a religion of punishment, it is worth analysing the merciful side of Islam.
Fyodor Gorovoy in the 1968–1972 Debates about the Date of Perm's Founding
Authors: Oleg Leybovich
Published: March 2023

On the basis of mostly published materials, the article investigates the socio-cultural context of the 1968 – 1972 debates about the foundation date of the city of Perm. The aim of the research is to clarify the significance of the debate for the self-determination of the affected part of the Perm public. The hypothesis is that it was conditioned by a change in cultural policy in the mid-1960s. The metaphor of the new cultural policy was the image of the small motherland saturated with value content. The idea of a second homeland was supposed to unite the socialist present times with the pre-Soviet past and fill the current ideology with symbols of the past, including the founding date of the city. Two cultural concepts collided in the discussion about the time of Perm's founding: professional and positivist ones presented by university rector F.S. Gorovoy, and mythmaking ideas voiced by journalist B.N. Nazarovsky. F.S. Gorovoy spoke on behalf of the Soviet science of that period, resorting to its accumulated methods. B.N. Nazarovsky appealed to compatriots' sense of dignity, insisting that his origins are from Ural foremen more worthy than those of imperial officials. The result of the ‘duel’ was decided by the Perm public, which was formed in the course of the public discussion – a gathering of active citizens, who expected from the discussion to satisfy their spiritual expectations, namely to create a colorful and convincing, bright myth about the past of the city of Perm. The "cold" academic narrative of F.S. Gorovoy ran counter to the expectations of the public opinion. For this reason, Gorovoy's arguments were ignored. A "Small Motherland" received a residence permit on the territory of Perm. Gorovoy disregarded these shifts in the public consciousness. The discussion about the timing of the city's creation revealed a certain cultural distance between the local intelligentsia and the official doctrine, with its primacy of science, rationality, and other similar properties. As Soviet citizens, Perm intellectuals were willing to trace their ancestry back to the artisans of Peter the Great's era rather than to the officials who flooded the city in response to Catherine the Great's decree. The argument over yesterday was won by the anger of today. The main result of the debates can be considered the revival of a relatively independent public opinion in the Soviet Perm. And it was so strong that it outweighed the professional historical arguments of F.S. Gorovoy.
Theatrical Life of the Inhabitants of Uralmash on the Pages of the Factory Newspaper in the Period From 1934 to 1939
Authors: Olga Staina
Published: March 2023

The article examines the theatrical life on the territory of the urban microdistrict Uralmash in Yekaterinburg, formerly known as Sverdlovsk. The Uralmash residential area was built in the period of the 30s of the 20th century, simultaneously with the Ural Heavy Machinery Plant "UZTM", a brand of the Soviet industry. The study provides an anthropological description of Uralmash in the 30s as a new type of settlement – "soc.the city". The analysis of theatrical events spanning from 1934 to 1939 in the conditions of constructing a social city reflects the growing interest of researchers to the processes of cultural construction during the first fiveyear plans and to the policy of the Soviet state aimed at formulating a new cultural standard. The purpose of the article is to characterize the theatrical life of the working district during the period of cultural construction. The research objectives are the following: to systematize and chronologically restore information about the theatrical events of the period of the 30s of the 20th century in Uralmash. The method of investigating the processes associated with the theatrical life of Uralmash is the analysis of 1934-1939 publications from the large-circulation newspaper "For Heavy Engineering". The article includes authentic texts from the factory edition. The materials of the newspaper "For Heavy Engineering" are conditionally divided into several blocks–"visiting performances", "tours", "amateur art", "organizational work", "reviews (including impressions and gratitude from the audience)", "performance announcements", "information about theater figures of the capital, republican and Sverdlovsk theaters"--and arranged chronologically . Theatrical life in the period from 1934 to 1939 at Uralmash was diverse, interesting and eventful. The strategy of building a cultural policy in the "sotsgorod" Uralmash was aimed at ensuring the accessibility of theatrical art by all categories of citizens. Theaters, as social institutions, built their activities and repertoire policy, correlating them with the slogan of that time – "Art should belong to the people." The factory's multi-edition "For Heavy Engineering" played a significant role in this process. Being the only mass media, the newspaper formed the theatrical culture of Uralmash residents. The newspaper provided coverage of events related to theatrical activity in all their aspects. The analysis makes it possible to restore the undeservedly forgotten history of cultural formation processes during the emergence of the Soviet society
Adults Living in Families That Experienced Food Insecurity in the Past 30 Days: United States, 2021
Authors: Weeks Julie , Mykyta Laryssa , Madans Jennifer
Published: March 2023

This Data Brief explores characteristics of adults aged 18 and over living in families experiencing food insecurity and reports the percentage of adults living in food-secure families by age, sex, race and Hispanic origin, disability, urbanization, and family composition.
OCENA PARAMETRÓW RUCHU BEZZAŁOGOWYCH PLATFORM LATAJĄCYCH
Authors: Falkowski Krzysztof , Ważny Mariusz , Henzel Maciej
Published: March 2023

W artykule przedstawione zostały wyniki badań weryfikacyjnych bezzałogowych statków powietrznych klasy micro opracowanych w Zakładzie Awioniki Wojskowej Akademii Technicznej (WAT). Opracowane statki powietrzne posiadają masę około 1 kg i są przeznaczone do wykorzystania przez służby porządkowe i ratownicze. Małe BSP muszą charakteryzować się podwyższonymi poziomami autonomii, ponieważ mogą operować nad skupiskami ludzi i infrastrukturą miejską. W artykule przedstawiona jest ocena stopnia automatyzacji, który decyduje o poziomie autonomii BSP.
Antibacterial Activity of Culinary-Medicinal Polypore Mushroom Lentinus tigrinus (Agaricomycetes)
Authors: Yazdi Yasaman , Rezaei Mohammad Reza , Pourianfar Hamid R.
Published: March 2023

Medicinal mushrooms belonging to Lentinus spp. exhibit significant antibacterial activities, but little attention has been paid to their efficacy against the food-borne pathogen, Bacillus cereus. The present study for the first time quantitatively evaluated the antibacterial activity of different extracts from fruiting bodies of a well-authenticated Iranian native strain of medicinal mushroom, Lentinus tigrinus, against Gram-positive spore-forming bacterium B. cereus. The findings revealed that the acetone extract inhibited the growth of B. cereus at concentrations as low as 31.25 μg/ML, while it had no effect against Escherichia coli and Staphylococcus aureus even at 10,000 μg/ML. The rest of the bacteria were also susceptible to the acetone extract at concentrations greater than 5 mg/ML. Antibacterial activities of the methanol-ethyl acetate extract and the hot water extract were significantly weaker than that of the acetone extract, which contained high amounts of total phenols (5.83 ± 0.08 mg GAE/g, dw), while Fourier transform infrared spectroscopy (FT-IR) confirmed the presence of functional groups, such as hydroxyl, carbonyl, amide, and amine. Further studies by scanning electron microscopy (SEM) confirmed obvious changes in the morphology of B. cereus in response to the acetone extract of L. tigrinus. This study may suggest that L. tigrinus could be a good natural source for isolating and purifying antibacterial compounds against B. cereus.
Epithelial Responses in Radiation-Induced Lung Injury (RILI) Allow Chronic Inflammation and Fibrogenesis
Authors: Beach Tyler A. , Finkelstein Jacob N. , Chang Polly Y.
Published: March 2023

Radiation models, such as whole thorax lung irradiation (WTLI) or partial-body irradiation (PBI) with bone-marrow sparing, have shown that affected lung tissue displays a continual progression of injury, often for months after the initial insult. Undoubtably, a variety of resident and infiltrating cell types either contribute to or fail to resolve this type of progressive injury, which in lung tissue, often develops into lethal and irreversible radiation-induced pulmonary fibrosis (RIPF), indicating a failure of the lung to return to a homeostatic state. Resident pulmonary epithelium, which are present at the time of irradiation and persist long after the initial insult, play a key role in the maintenance of homeostatic conditions in the lung and have often been described as contributing to the progression of radiation-induced lung injury (RILI). In this study, we took an unbiased approach through RNA sequencing to determine the in vivo response of the lung epithelium in the progression of RIPF. In our methodology, we isolated CD326+ epithelium from the lungs of 12.5 Gy WTLI C57BL/6J female mice (aged 8–10 weeks and sacrificed at regular intervals) and compared irradiated and non-irradiated CD326+ cells and whole lung tissue. We subsequently verified our findings by qPCR and immunohistochemistry. Transcripts associated with epithelial regulation of immune responses and fibroblast activation were significantly reduced in irradiated animals at 4 weeks postirradiation. Additionally, alveolar type-2 epithelial cells (AEC2) appeared to be significantly reduced in number at 4 weeks and thereafter based on the diminished expression of pro-surfactant protein C (pro-SPC). This change is associated with a reduction of Cd200 and cyclooxygenase 2 (COX2), which are expressed within the CD326 populations of cells and function to suppress macrophage and fibroblast activation under steady-state conditions, respectively. These data indicate that either preventing epithelial cell loss that occurs after irradiation or replacing important mediators of immune and fibroblast activity produced by the epithelium are potentially important strategies for preventing or treating this unique injury.
Relational analysis between economic activities and mobility during coronavirus disease
Authors: Jang Yoonjung , Ku Donggyun , Choi Minje , Kim Dahye , Lee Doyun , Lee Seungjae
Published: March 2023

The coronavirus disease (Covid-19) has significantly affected daily lives since it was declared a pandemic in March 2020 by the World Health Organization. Studies in Korea to interpret the relationship between Covid-19 and transportation showed that there has been an overall decrease in the use of public transportation, with a significant decrease when the infection was widespread. In addition, Covid-19 has also affected the commercial environment. In particular, the number of people shopping in commercial districts such as retail outlets has decreased significantly. This study analyses the disease vectors (spread factors) of Covid-19 in terms of transportation demand, the revitalisation of commercial districts, living populations and socio-economic indicators. It attempts to identify different infection factors for each district in Seoul using a causal analysis methodology partial least-squares structural equation modelling such that Covid-19 can be managed continuously, with the aim to provide a foundation for pre-emptive actions by adjusting or controlling specific influencing factors pertaining to infectious diseases.
3D finite-element analysis of innovative coconut palm stem shaped headed shear connectors
Authors: Pardeshi Rahul Tarachand , Patil Yogesh Deoram
Published: March 2023

Headed studs are shear connectors in composite structures used at the adjoining face of a steel beam and a concrete slab. In this research, the conventional shape of a headed stud was restructured to resemble the shape of a coconut palm stem (royal palm) without a change in the overall material volume, with the aim of improving the shear strength of the composite connection. Six innovative shear connectors for composite structures, named coconut palm stem royal shaped headed stud shear connectors (CPSR studs), were examined. Abaqus/Explicit was used to model a push-out specimen and the finite-element model was successfully validated using published experimental results. The six different CPSR studs encased in three grades of concrete (C40, C50 and C60) were tested for shear strength, stiffness and load–slip performance. The results for the innovative CPSR studs were compared with those for uniform cross-section headed studs; when embedded in C40, C50 and C60 grade concrete, the results showed, respectively, 35–41%, 37–44% and 41–52% improvements in the ultimate strength of the shear connection. The improved shear strength capacity of the CPSR studs without a change in the overall volume of stud material means that fewer studs are required, leading to economic benefits.
Effects of Trait Dominance on Social Rank Achievement in the Workplace
Authors:   The Korea International Trade Research Institute, Yoon Hye-Jung
Published: March 2023

Purpose – The purpose of this paper is to identify, explore, and provide the underlying mechanisms for an individual’s trait dominance leading to a high social standing within a group. Design/Methodology/Approach – Based on status characteristics theory, implicit theories, and social exchange theory, this study posits that informal leadership is the main mediator in the relationship between trait dominance and social rank enhancement. This study also argues that the relationship between trait dominance and perceived informal leadership diminishes when trait dominance is not accompanied by a corresponding level of competence. It also argues that the positive association between trait dominance and perceived informal leadership is amplified when the group feels a sense of urgency. Using a survey-based study with 270 employee–peer pairs, this study tested hypotheses using a series of hierarchical regressions. Findings – This paper studies and empirically finds the indirect mediating effect of informal leadership connecting trait dominance to social rank. Moreover, the moderating effect of sense of urgency is found as statistically significant. That is, when group members perceive that the group encounters uncertain or even risky situations, they appreciate the value of trait dominance possessed by informal leaders. Research Implications – In the management of this paper, this study sheds light on the inconsistent effects of dominance on social rank by providing an alternative pathway. The current theoretical and empirical research framework emphasizes that individuals with trait dominance are perceived to be leadership material regardless of their official title as they possess and convey various leadership-signaling cues.
An Analysis of Korea-UK FTA Improvement Negotiations and the New Trade Environment
Authors:   The Korea International Trade Research Institute, Kim Jong-Kwon
Published: March 2023

Purpose – The aim of this analysis is to provide implications for trade practice by analyzing FTA progress in the digital economy and trade field with IPEF through FTA improvement negotiations between Korea and the UK, and an analysis of the new trade environment. Design/Methodology/Approach – The annual export/import growth rate (year-on-year) before and after the Korea-UK FTA was concluded and forecasts for 2022 and 2023 were reviewed. This data from 2000 to 2021 are based on the Economic Statistical System of the Bank of Korea. It recorded 33.20% in 2021, the second highest growth rate since the 2000s, after recording 36.95% in 2004. It was estimated through the model of ARIMA (1, 1, 0) in the case of 2022 and 2023. After the Korea-UK FTA was concluded, it was found that the trade volume of Korea-UK combined exports and imports increased. Findings – In the future, it is expected that joint cooperation and R&D investment in high-tech and hightech industries will be more actively developed through improvement negotiations in the fields of SCM, digital economy, and trade. Research Implications – The US is currently emphasizing US production of optical fibers, semiconductors, and electric vehicles as well as key parts in relation to the global supply chain. Therefore, the Korea-UK FTA improvement negotiation and framework require joint cooperation and R&D investment in the reorganization of the US global supply chain in cutting-edge industries, including US semiconductors, science law, and inflation reduction laws.
A Study on the Segmentation of Korean Agri-Food Imports in South-East Asian Countries: Focusing on a Comparison among GDP Per Capita, the Top 10% GDP Per Capita, and Top 1% GDP Per Capita
Authors:   The Korea International Trade Research Institute, Chung Dae-Hee , Seok Jun-Ho
Published: March 2023

Purpose – The objective of this paper is to test whether low income countries in South-East Asian countries can import the high price and quality agri-food products of South Korea. Specifically, we divide consumers in these regions based on income level (Average, Top 10%, and Top 1% GDP per capita) to examine the exportability of those goods to South-East Asian countries. Design/Methodology/Approach – Utilizing the panel Granger causality test method of Dumitrescu and Hurlin (2012), we examine which income segments in those regions can consume the high price, quality and agri-food products of Korea. Additionally, this study also uses the panel impulse-response function to figure out the which segments are more inclined to buy agri-food products of Korea. Findings – Results of the panel Granger causality test show that the incomes of only the top 10% and 1% segments of these countries lead to agri-food imports from Korea. Results of the panel impulse response function report that the top 1% segment of income group imports more compared to the top 10% segments of the income group. Research Implications – Our results imply that high quality and high price agri-food products of Korea can be exported to low-income South and South-East Asian countries. Our approach also presents that the gravity model specification smight need to be modified according to the characteristics of products.
Transitional regimes and stability of electric power systems
Authors:   Novosibirsk State Technical University, Russian Federation, Dolgov Alexandr P
Published: March 2023

The issues of parallel operation of electrical systems are considered. Some major system crashes are described. The peculiarities of the electromechanical transition process in the electric power system are reflected. Angular characteristics of generator power, static characteristics of asynchronous motors and complex loads are analyzed. The main methods of analyzing the stability of electric power systems are described. The asynchronous mode is considered. Information on emergency automation used in modern conditions is given. The Park–Gorev equations are given; their place in the calculation of electromechanical transients of synchronous and asynchronous machines is mentioned. The issues of self-starting synchronous and asynchronous motors are considered. The material is accompanied by examples and control questions. The textbook meets the requirements of the State Educational Standard of Higher Professional Education for the preparation of bachelors in the direction "Electric Power and Electrical Engineering" (13.03.02 ) and masters in the direction "Electric Power and Electrical Engineering" (13.04.02). It can also be useful for graduate students.
ВЛИЯНИЕ ГОСУДАРСТВЕННО-ЧАСТНОГО ПАРТНЕРСТВА НА РЕГИОНАЛЬНОЕ РАЗВИТИЕ
Authors: Глазунова Вильгельмина Витальевна
Published: March 2023

Целью исследования является анализ развития рынка классического государственно-частного партнерства (ГЧП) в регионах в сферах ЖКХ, социальной, транспортной и IT-сфере с позиции оценки влияния на развитие регионов. В связи с тем, что повышение уровня инфраструктурного развития в данных сферах лежит в основе социально-экономического подъема, объемы инвестиций в инфраструктуру можно рассматривать как факторы экономического роста в регионах. Стоит отметить, что для реализации крупных проектов по развитию инфраструктуры используется именно механизм ГЧП, обеспечивающий осуществление проектов в рамках национальных программ.

Методология исследования основана на анализе динамических рядов по объемам инвестирования в сферах ЖКХ, социальной, транспортной и IT-сфере, при этом данные приводятся к базовому периоду — 2006 г. На их основе выстроены диаграммы, характеризующие объемы инвестирования с помощью ГЧП в вышеуказанных сферах, а также отмечены наиболее крупные проекты ГЧП, оказавшие значительное влияние на развитие регионов.

Результат исследования состоит в оценке развития сфер ЖКХ, социальной, транспортной и IT-сферы в регионах РФ как основы для их экономического роста. Базой для анализа послужили объемы инвестиций, вложенные в развитие регионов посредством механизма классического ГЧП.
МАКРОЭКОНОМИЧЕСКАЯ ПОЛИТИКА: НАКОПИТЕЛЬНЫЙ ЭФФЕКТ И ЕГО ПРЕОДОЛЕНИЕ
Authors: Сухарев Олег Сергеевич
Published: March 2023

Макроэкономическая политика как система воздействий на экономику демонстрирует накопительный эффект, который выражается в потере чувствительности цели к инструментам политики (негативный), либо в увеличении этой чувствительности (позитивный). Тем самым применение повторяющихся многократно методов политики может не влиять на изменение целей развития. Сила и повторяемость применения инструмента имеет принципиальное значение и не учитывается классической экономической теорией.

Цель исследования — сформировать представления об отрицательном накопительном эффекте макроэкономической политики, расширив принципы «цели-инструменты» и «эффективной рыночной классификации» на базе авторской доктрины «распределённого управления», позволяющей дифференцировать инструменты политики и учесть накопительный эффект, как ситуацию, когда чувствительность целей развития к инструментам может снижаться (увеличиваться).

Методология исследования — построена на классической теории экономической политики Тинбергена-Манделла, эмпирическом и структурном анализе.

Общий результат проведенного исследования сводится к демонстрации накопительного эффекта макроэкономической политики, обнаруживаемого для российской экономики на уровне общей политики роста и по целям структурного развития. Повышение чувствительности целей развития к методам политики видится в области нескольких вариантов решений: замены применяемых инструментов раздельно и в совокупности — смена парадигмы политики, а также повышение силы и действенности применяемого инструмента. В частности, помимо использования созданного и отложенного дохода, требуется учесть ресурсы, сконцентрированные в секторах экономики, которые могут быть использованы для новой модели роста при соответствующей структурной политике.
БЕЗОПАСНОСТЬ ЛИЧНОСТИ В СОВРЕМЕННОЙ ГЛОБАЛЬНОЙ СЕТИ
Authors: Дементьев Сергей Александрович
Published: March 2023

Цель статьи заключается в изучении особенностей обеспечения безопасности личности в глобальной сети Интернет.

Результаты исследования. Автор сосредотачивает внимание на различиях сетевой и реальной личности индивида и делает вывод о том, что отношение к собственной безопасности значительно отличается в виртуальной реальности. Объясняется это технической опосредованностью и асинхронным характером сетевой коммуникации. Безопасность личности в Интернете связывается, в первую очередь, с безопасностью личных данных и позиционируется как фундамент комфортной жизнедеятельности. Автор анализирует риски, с которыми сталкивается пользователь при внесении своих персональных данных в социальные сети на примере групп, имеющих интерес к конфиденциальным данным пользователей. Сделан вывод о том, что этикет общения в глобальной сети и низкая интернет-грамотность является важнейшим фактором в повышении рискогенности интернет-коммуникаций.

Перспективы исследования заключаются в дальнейшем осмыслении механизмов обеспечения безопасности личности в цифровом пространстве.
ГОРОД В ПРЕДМЕТНОМ ПРОСТРАНСТВЕ СОЦИАЛЬНО-ГУМАНИТАРНОГО ЗНАНИЯ: ИСТОРИЧЕСКИЕ И СОВРЕМЕННЫЕ АСПЕКТЫ ИССЛЕДОВАНИЯ
Authors: Козловский Максим Евгеньевич
Published: March 2023

Цель исследования заключается в анализе походов к проблеме города, сложившихся в социально-гуманитарном знании.

Методологической основой исследования выступают социокультурный, символический и междисциплинарный подходы.

Результаты исследования. Развитие человечества идет преимущественно по вектору урбанизации. Сегодняшнее развитие города как социокультурного пространства несет в себе позитивные тенденции, связанные с наличием широкого диапазона возможностей для самореализации личности, с повышением качества жизни, созданием комфортной среды, и экологические и экзистенциальные риски для человека.

Перспективы исследования. Работа открывает перспективы для дальнейшего исследования тенденций в развитии современного города.
ВОСЕМЬ ВАРИАНТОВ ДЕТЕРМИНАЦИИ РОСТОМ ВВП ЕСТЕСТВЕННОГО ДВИЖЕНИЯ НАСЕЛЕНИЯ (НА ПРИМЕРЕ СТАТИСТИЧЕСКИХ ДАННЫХ РОССИЙСКОЙ ФЕДЕРАЦИИ)
Authors: Лукичев Павел Николаевич
Published: March 2023

Целью исследования является построение моделей стохастической связи роста валового внутреннего продукта и изменения численности населения.

Методологическим основанием для понимания такой связи выступает математическая модель зависимости коэффициента прироста населения от наличествующего объема экологической ниши популяции и энергетической насыщенности культурных форм удовлетворения витальных потребностей социальных индивидов. Предлагаемая методология позволяет осуществлять прогнозирование естественного движения населения, что основывается на трех постулатах: тенденции к экспоненциальному росту народонаселения, зависимости темпов роста народонаселения от объема свободного остатка экологической ниши популяции, динамической взаимосвязи между рождаемостью и смертностью.

Результаты исследования. На этой основе сделаны логические выводы о демографическом исчислении прироста совокупности потребленных стоимостей, соотносимого в процентном выражении с ростом валового внутреннего продукта. В результате разработанная модель получает как прогностическое, так и верификационное значение.

Перспективы исследования. Дальнейшей перспективой исследований является формирование идей новой политической экономии, учитывающих единство экономических и демографических процессов.
ПУБЛИКАЦИОННАЯ КУЛЬТУРА СОВРЕМЕННОГО УЧЕНОГО: ПОСТАНОВКА ПРОБЛЕМЫ
Authors: Сальникова Инна Ивановна
Published: March 2023

Статья посвящена исследованию проявления публикационной культуры современного ученого в его публикационной деятельности.

Цель статьи — показать, что формирование имиджа ученого напрямую связано с его умением донести результаты своих исследований до научного сообщества через публикации в научных журналах, то есть с его публикационной культурой.

Методологией исследования являются идеи и принципы, содержащихся в работах российских ученых, посвященных рассмотрению различных аспектов формирования имиджа и репутации ученых на современном этапе развития науки и высшего образования.

Результатами работы стало выявление произошедших изменений в формировании рейтинга ученых в научном сообществе и роли в этом процессе публикационной культуры.

Перспективой исследования является установление корреляции репутации научных работников и таких показателей, как публикационная активность и публикационный этос.
МЕТОДИКА ОЦЕНКИ ЭФФЕКТИВНОСТИ ПРОФЕССИОНАЛЬНОЙ ПЕДАГОГИЧЕСКОЙ ДЕЯТЕЛЬНОСТИ
Authors: Тищенко Юлия Геннадьевна , Ковалева Наталья Юрьевна , Тищенко Ирина Александровна
Published: March 2023

Цель исследования состоит в разработке методики оценки эффективности профессиональной педагогической деятельности по ее конечным результатам — психологическому и функциональному, выступающим в качестве критериев ее (деятельности) эффективности.

Методологическая база исследования — общая теория функционирования педагогических систем Н. В. Кузьминой, методы системно-структурного подхода к анализу педагогических явлений.

Результаты исследования. Разработана методика оценки эффективности профессиональной педагогической деятельности: балльные шкалы для оценки психологического и функционального результатов педагогической деятельности, методика их (результатов) оценивания.

Перспективы исследования: разработка балльных шкал для оценки психологического и функционального результатов деятельности педагогов различных учебных дисциплин разных ступеней системы образования.
SEcuRe 2.0 – A simple and economic protocol for efficient in vitro fertilization using cryopreserved mouse sperm v4
Authors: Wigger Magdalena
Published: March 2023

The advent of genome editing tools like CRISPR/Cas has substantially increased the number of genetically engineered mouse models in recent years. In support of refinement and reduction, sperm cryopreservation is advantageous compared to embryo freezing for archiving and distribution of such mouse models. The in vitro fertilization using cryopreserved sperm from the most widely used C57BL/6 strain has become highly efficient in recent years due to several improvements of the procedure. However, the purchase of the necessary media for routine application of the current protocol poses a constant burden on budgetary constraints. In-house media preparation, instead, is complex and requires quality control of each batch. Here, we describe SEcuRe 2.0, a cost-effective and easily adaptable approach for in vitro fertilization using cryopreserved C57BL/6 sperm. This is mainly achieved by modification of an affordable commercial fertilization medium and a step-by-step description of all other necessary reagents. This protocol is compatible with frozen sperm from all major repositories, and the IVF can easily be adapted to accommodate freshly harvested sperm. The basal fertilization medium (Cook's RVF) used in the initial SEcuRe approach has been discontinued by the manufacturer. We have validated HTF as a replacement, and SEcuRe 2.0 successfully employs HTF as the basal fertilization medium ever since. (The last step contains a supplemental video with extra context and tips, as part of the protocols.io Spotlight series, featuring conversations with protocol authors.)
Descriptive analysis of patient involvement in the development of German clinical practice guidelines: A meta-research study v1
Authors: Pfisterer-Heise Stefanie
Published: March 2023

Clinical practice guidelines include recommendations to optimize patient care. As patients are "experts by experience" for their medical conditions, their involvement in the development of clinical practice guidelines is essential. Accordingly, many organizations worldwide require patient involvement in the development of clinical practice guidelines. However, in 2018 a German study found that patient involvement in the development of German clinical practice guidelines still lagged significantly behind international requirements. This meta research-study is an update on the status of patient involvement in German clinical practice guidelines. It examines whether and how patients have been involved in the development of currently valid German clinical practice guidelines as well as the current status of patient versions of German clinical practice guidelines. Furthermore, this meta-research study investigates in how far German clinical practice guidelines follow the RIGHT Checklist with respect to the reporting of patient involvement. To this aim the German clinical practice guidelines register will be searched for currently valid S3 clinical practice guidelines which will be screened by two independent reviewers. Relevant data on patient involvement will be extracted and analyzed descriptively. The results will be compared to the previous German study.
Visualization of Yeast Chromosomes Using Clamped Homogeneous Electric Field (CHEF) Electrophoresis v1
Authors: L. Hoyer Lois
Published: March 2023

I inherited this protocol while working as a postdoctoral researcher in the laboratory of Dr. Stewart Scherer in the 1990s. Literature that influenced protocol development is listed in the References section. This protocol describes how to isolate chromosome-sized DNA from yeast cells. The yeast cells are encased in agarose, then the cell is digested away. The result is unsheared chromosomes in an agarose plug that can be loaded onto electrophoresis gels or subjected to additional analysis such as digestion with restriction enzymes. The protocol details two sets of electrophoresis conditions for separating the chromosomes using a BioRad CHEF-DR III variable angle electrophoresis system. Because the protocol was developed for work with Candida albicans, the conditions are most useful for chromosomes in the size range of approximately 0.5 to 5 Mb. The "short protocol" (Condition 1) separates the smaller C. albicans chromosomes (approximately 1 Mb) without running them off the bottom of the gel. The "long protocol" (Condition 2) separates the larger chromosomes (approximately 3 Mb) with approximately 0.1 Mb resolution but risks running smaller DNA molecules off the gel. We typically run samples using both electrophoresis protocols and combine the resulting information to understand the karyotype.
Viral PEG purification v1
Authors: not provided sarah.schulz
Published: March 2023

Protocol for viral particle purification via PEG percipitation
Slide-TCR-Seq v3 (IVT) v1
Authors: Liu Sophia
Published: March 2023

T cells mediate antigen-specific immune responses to disease through the specificity and diversity of their clonotypic Tcell receptors (TCRs). Determining the spatial distributions of Tcell clonotypes in tissues is essential to understanding Tcell behavior, but spatial sequencing methods remain unable to profile the TCR repertoire. We developed Slide-TCR-seq, a 10-μm-resolution method, to sequence whole transcriptomes and TCRs within intact tissues. Our method yields insights into the spatial relationships between clonality, neighboring cell types, and gene expression that drive Tcell responses. The most recent version of our protocol uses in vitro transcription in lieu of rhPCR amplfiication, which overcomes the barcode switching introduced by the rhPCR and results in higher mapping rates.
Slide-TCR-Seq v3 (IVT) v2
Authors: Liu Sophia
Published: March 2023

T cells mediate antigen-specific immune responses to disease through the specificity and diversity of their clonotypic Tcell receptors (TCRs). Determining the spatial distributions of Tcell clonotypes in tissues is essential to understanding Tcell behavior, but spatial sequencing methods remain unable to profile the TCR repertoire. We developed Slide-TCR-seq, a 10-μm-resolution method, to sequence whole transcriptomes and TCRs within intact tissues. Our method yields insights into the spatial relationships between clonality, neighboring cell types, and gene expression that drive Tcell responses. The most recent version of our protocol uses in vitro transcription in lieu of rhPCR amplification, which overcomes the barcode switching introduced by the rhPCR and results in higher mapping rates.
Cómo son y qué hacen los buenos profesores. Sus voces y las de sus estudiantes
Authors:   Universidad de Antioquia, Gaviria Cortés Didier Fernando , Chaverra Fernández Beatriz Elena ,   Universidad de Antioquia, Ospina Ospina Enoc Valentín ,   Universidad de Antioquia, Uribe Pareja Ivan Darío ,   Universidad de Antioquia, Muriel Echavarría Johan Manuel ,   Universidad de Antioquia, Moreno López Juan David ,   Institución Educativa San José Obrero, Cardona Mejía Liliana María ,   Universidad de Antioquia, Bustamante Castaño Sirley Andrea ,   Universidad de Antioquia
Published: March 2023

El propósito de este libro está relacionado con la comprensión de las prácticas de enseñanza de los buenos profesores desde la voz de los estudiantes. Por tanto, se abordan algunos interrogantes como: ¿solo basta el conocimiento para ser un buen profesor o es importante la vocación, la didáctica y la pedagogía?, ¿cómo se han hecho los buenos profesores?, ¿cuáles experiencias profesionales, personales y sociales le aportan a su quehacer como profesores?, ¿por qué es importante ser un buen profesor?, ¿qué factores o dinámicas influyen en la práctica docente?, ¿cómo preparan sus clases los buenos profesores?, ¿qué esperan de sus estudiantes?, ¿qué piensan de la educación y su institución?, ¿cómo hacen sus clases?, ¿cómo se relacionan con los estudiantes los buenos profesores?, ¿cómo son los buenos profesores en la clase y fuera de esta?, ¿qué los motiva a ser diferentes de los demás profesores de sus instituciones?, ¿cuáles son las percepciones que tienen los estudiantes sobre sus profesores?, ¿cuáles son las características y valores de un buen profesor? Este estudio trata de rescatar a la persona de la práctica pedagógica, con el fin de devolver, recuperar, resaltar y hacer visibles la voz y la presencia que el profesorado tiene como trabajador de la cultura. Además, reconocerle como objeto de conocimiento y transformación social.
Experience of successful use of immunotherapy in treatment of locally advanced inoperable squamous cell carcinoma of the hairy part of the head
Authors: Pkheshkhova B. G. , Mudunov A. M. , Pak M. B. , Zeynalova P. A. , Batsev A. F.
Published: March 2023

This article presents the experience of successful use of immunotherapy in treatment of locally advanced inoperable squamous cell carcinoma of the hairy part of the head.