bluuebunny/crossref_metadata_2025 huggingface dataset:
A Sustainable Autoclaved Material Made of Glass Sand
Authors: Stepien Anna , Leśniak Magdalena , SITARZ Maciej
Published: November 2019

Far-reaching technological progress, manufacturing, and rapidly advancing globalization dictate new conditions for the development and changes in the construction industry. Valorization of by-products and the use of secondary materials in the production of building materials have attracted a lot of attention. Silicate materials were assessed on the basis of their compressive property. An orthogonal compositional plan type 3k (with k = 2), that is, a full two-factor experiment was applied in order to carry out the compressive strength and bulk density tests. Glass sand was added to the silicate mass as a modification. The results show that the compressive strength was higher than that of traditional bricks. Scanning electron microscopy coupled with energy dispersive spectrometry SEM/EDS was used to study the microstructure, whereas the XRD analysis was applied to examine the structures. Laboratory tests were performed on samples with dimensions of 50 × 50 × 50 mm. The results show the bulk density increase to the value of 1.75 kg/dm3, which increases the acoustic performance of the new products. The results of the modifications also indicate changes in the structure of the new bricks. The reference sample contained α-quartz, zeolite, tobermorite 9A, and calcium aluminum silicate (Ca2Al4Si12O32), whereas the samples modified with glass sand, the presence of phases such as α-cristobalite, natrolite, tobermorite 11A, gyrolite, and analcite was recorded.
Histone Demethylase KDM4C Stimulates the Proliferation of Prostate Cancer Cells via Activation of AKT and c-Myc
Authors: Lin Ching-Yu , Wang Bi-Juan , Chen Bo-Chih , Tseng Jen-Chih , Jiang Shih Sheng , Tsai Kelvin K. , Shen Ying-Ying , Yuh Chiou Hwa , Sie Zong-Lin , Wang Wen-Ching , Kung Hsing-Jien , Chuu Chih-Pin
Published: November 2019

Our three-dimensional organotypic culture revealed that human histone demethylase (KDM) 4C, a histone lysine demethylase, hindered the acini morphogenesis of RWPE-1 prostate cells, suggesting its potential oncogenic role. Knockdown (KD) of KDM4C suppressed cell proliferation, soft agar colony formation, and androgen receptor (AR) transcriptional activity in PCa cells as well as reduced tumor growth of human PCa cells in zebrafish xenotransplantation assay. Micro-Western array (MWA) analysis indicated that KD of KDM4C protein decreased the phosphorylation of AKT, c-Myc, AR, mTOR, PDK1, phospho-PDK1 S241, KDM8, and proteins involved in cell cycle regulators, while it increased the expression of PTEN. Fluorescent microscopy revealed that KDM4C co-localized with AR and c-Myc in the nuclei of PCa cells. Overexpression of either AKT or c-Myc rescued the suppressive effect of KDM4C KD on PCa cell proliferation. Echoing the above findings, the mRNA and protein expression of KDM4C was higher in human prostate tumor tissues as compared to adjacent normal prostate tissues, and higher KDM4C protein expression in prostate tumors correlated to higher protein expression level of AKT and c-Myc. In conclusion, KDM4C promotes the proliferation of PCa cells via activation of c-Myc and AKT.
Therapeutic Inducers of Apoptosis in Ovarian Cancer
Authors: Binju Mudra , Amaya-Padilla Monica Angelica , Wan Graeme , Gunosewoyo Hendra , Suryo Rahmanto Yohan , Yu Yu
Published: November 2019

Ovarian cancers remain one of the most common causes of gynecologic cancer-related death in women worldwide. The standard treatment comprises platinum-based chemotherapy, and most tumors develop resistance to therapeutic drugs. One mechanism of developing drug resistance is alterations of molecules involved in apoptosis, ultimately assisting in the cells’ capability to evade death. Thus, there is a need to focus on identifying potential drugs that restore apoptosis in cancer cells. Here, we discuss the major inducers of apoptosis mediated through various mechanisms and their usefulness as potential future treatment options for ovarian cancer. Broadly, they can target the apoptotic pathways directly or affect apoptosis indirectly through major cancer-pathways in cells. The direct apoptotic targets include the Bcl-2 family of proteins and the inhibitor of apoptotic proteins (IAPs). However, indirect targets include processes related to homologous recombination DNA repair, micro-RNA, and p53 mutation. Besides, apoptosis inducers may also disturb major pathways converging into apoptotic signals including janus kinase (JAK)/signal transducer and activator of transcription 3 (STAT3), wingless-related integration site (Wnt)/β-Catenin, mesenchymal-epithelial transition factor (MET)/hepatocyte growth factor (HGF), mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK), and phosphatidylinositol 3-kinase (PI3K)/v-AKT murine thymoma viral oncogene homologue (AKT)/mammalian target of rapamycin (mTOR) pathways. Several drugs in our review are undergoing clinical trials, for example, birinapant, DEBIO-1143, Alisertib, and other small molecules are in preclinical investigations showing promising results in combination with chemotherapy. Molecules that exhibit better efficacy in the treatment of chemo-resistant cancer cells are of interest but require more extensive preclinical and clinical evaluation.
Animal Models of Hepatocellular Carcinoma Prevention
Authors: Shankaraiah Ram C. , Gramantieri Laura , Fornari Francesca , Sabbioni Silvia , Callegari Elisa , Negrini Massimo
Published: November 2019

Hepatocellular carcinoma (HCC) is a deadly disease and therapeutic efficacy in advanced HCC is limited. Since progression of chronic liver disease to HCC involves a long latency period of a few decades, a significant window of therapeutic opportunities exists for prevention of HCC and improve patient prognosis. Nonetheless, there has been no clinical advancement in instituting HCC chemopreventive strategies. Some of the major challenges are heterogenous genetic aberrations of HCC, significant modulation of tumor microenvironment and incomplete understanding of HCC tumorigenesis. To this end, animal models of HCC are valuable tools to evaluate biology of tumor initiation and progression with specific insight into molecular and genetic mechanisms involved. In this review, we describe various animal models of HCC that facilitate effective ways to study therapeutic prevention strategies that have translational potential to be evaluated in a clinical context.
Performance of a Ni-Cu-Co/Al2O3 Catalyst on in-situ Hydrodeoxygenation of Bio-derived Phenol
Authors: Xue Huiyuan , Xu Jingjing , Gong Xingxing , Hu Rongrong
Published: November 2019

The in-situ hydrodeoxygenation of bio-derived phenol is an attractive routine for upgrading bio-oils. Herein, an active trimetallic Ni-Cu-Co/Al2O3 catalyst was prepared and applied in the in-situ hydrodeoxygenation of bio-derived phenol. Comparison with the monometallic Ni/Al2O3 catalyst and the bimetallic Ni-Co/Al2O3 and Ni-Cu/Al2O3 catalysts, the Ni-Cu-Co/Al2O3 catalyst exhibited the highest catalytic activity because of the formation of Ni-Cu-Co alloy on the catalyst characterized by using X-ray powder diffraction (XRD), temperature programmed reduction (TPR), N2 physisorption, scanning electron microscope (SEM), and transmission electron microscope (TEM). The phenol conversion of 100% and the cyclohexane yield of 98.3% could be achieved in the in-situ hydrodeoxygenation of phenol at 240 °C and 4 MPa N2 for 6 h. The synergistic effects of Ni with Cu and Co of the trimetallic Ni-Cu-Co/Al2O3 catalyst played a significant role in the in-situ hydrodeoxygenation process of phenol, which not only had a positive effect on the production of hydrogen but also owned an excellent hydrogenolysis activity to accelerate the conversion of cyclohexanol to cyclohexane. Furthermore, the catalyst also exhibited excellent recyclability and good potential for the upgrading of bio-oils.
Study on the Catalytic Pyrolysis Mechanism of Lignite by Using Extracts as Model Compounds
Authors: Liu Jianwei , Zhang Qian , Liang Litong , Huang Wei
Published: November 2019

Understanding the catalytic pyrolysis mechanism of lignite is of great significance for obtaining a high yield of the target products or designing high-efficiency catalysts, which are generally derived by using simple model compounds, while the ordinary model compounds cannot represent the real atmosphere of lignite pyrolysis owing to the simple structures and single reactions. Based on the coal two-phase model, the extractable compounds are the important compositions of coal, which can reflect the partial characteristics of raw coal while obtaining a high extraction yield. Hence, a better understanding of the interaction between the coal structure and catalyst can be inferred by using a mobile phase in coal as model compounds instead of conventional simple compounds. In this work, tetrahydrofuran extracts of lignite were chosen as model compounds to study the catalytic pyrolysis mechanism with separate addition of Fe(NO3)3 and FeCl3 by using a thermogravimetric combined with mass spectrometry. It was found that about 77.88% of the extracts were vaporized before 700 °C, and the residual yield was 22.12%. With the separate addition of 5 wt % of Fe(NO3)3 and FeCl3, the conversion of the extracts increased to 84.38% and 89.66%. Meanwhile, the final temperature decreased to 650 and 550 °C, respectively. The addition of Fe(NO3)3 and FeCl3 promoted the breakage of aliphatic chains at approximately 150 °C, leading to the generation of CH4 and H2 in the temperature range 100–200 °C, which were nearly invisible for that without catalyst. The addition of iron-based catalysts allowed more CO2 formation at approximately 200 °C since they enabled efficient promotion of the cleavage of carboxyl functionals at lower temperatures. The enlarged peak of H2O and CH4 at approximately 500 °C means that iron-based catalysts are significant for the cleavage of methoxy groups in the catalytic respect. Aromatic side chains facilitated cracking at approximately 500 °C, leading to more light aliphatics and aromatics generation in this temperature range.
Physical Exercise and Selective Autophagy: Benefit and Risk on Cardiovascular Health
Authors: Wu Ne N. , Tian Haili , Chen Peijie , Wang Dan , Ren Jun , Zhang Yingmei
Published: November 2019

Physical exercise promotes cardiorespiratory fitness, and is considered the mainstream of non-pharmacological therapies along with lifestyle modification for various chronic diseases, in particular cardiovascular diseases. Physical exercise may positively affect various cardiovascular risk factors including body weight, blood pressure, insulin sensitivity, lipid and glucose metabolism, heart function, endothelial function, and body fat composition. With the ever-rising prevalence of obesity and other types of metabolic diseases, as well as sedentary lifestyle, regular exercise of moderate intensity has been indicated to benefit cardiovascular health and reduce overall disease mortality. Exercise offers a wide cadre of favorable responses in the cardiovascular system such as improved dynamics of the cardiovascular system, reduced prevalence of coronary heart diseases and cardiomyopathies, enhanced cardiac reserve capacity, and autonomic regulation. Ample clinical and experimental evidence has indicated an emerging role for autophagy, a conservative catabolism process to degrade and recycle cellular organelles and nutrients, in exercise training-offered cardiovascular benefits. Regular physical exercise as a unique form of physiological stress is capable of triggering adaptation while autophagy in particular selective autophagy seems to be permissive to such cardiovascular adaptation. Here in this mini-review, we will summarize the role for autophagy in particular mitochondrial selective autophagy namely mitophagy in the benefit versus risk of physical exercise on cardiovascular function.
Human iPSCs-Derived Endothelial Cells with Mutation in HNF1A as a Model of Maturity-Onset Diabetes of the Young
Authors: Kachamakova-Trojanowska Neli , Stepniewski Jacek , Dulak Jozef
Published: November 2019

Patients with HNF1A-maturity-onset diabetes of the young (MODY) often develop endothelial dysfunction and related microvascular complications, like retinopathy. As the clinical phenotype of HNF1A-MODY diabetes varies considerably, we used human induced pluripotent stem cells (hiPSCs) from two healthy individuals (control) to generate isogenic lines with mutation in HNF1A gene. Subsequently, control hiPSCs and their respective HNF1A clones were differentiated toward endothelial cells (hiPSC-ECs) and different markers/functions were compared. Human iPSC-ECs from all cell lines showed similar expression of CD31 and Tie-2. VE-cadherin expression was lower in HNF1A-mutated isogenic lines, but only in clones derived from one control hiPSCs. In the other isogenic set and cells derived from HNF1A-MODY patients, no difference in VE-cadherin expression was observed, suggesting the impact of the genetic background on this endothelial marker. All tested hiPSC-ECs showed an expected angiogenic response regardless of the mutation introduced. Isogenic hiPSC-ECs responded similarly to stimulation with pro-inflammatory cytokine TNF-α with the increase in ICAM-1 and permeability, however, HNF1A mutated hiPSC-ECs showed higher permeability in comparison to the control cells. Summarizing, both mono- and biallelic mutations of HNF1A in hiPSC-ECs lead to increased permeability in response to TNF-α in normal glycemic conditions, which may have relevance to HNF1A-MODY microvascular complications.
Superhydrophobic Civil Engineering Materials: A Review from Recent Developments
Authors: Xiang Tengfei , Lv Zhong , Wei Feifei , Liu Jian , Dong Wei , Li Cheng , Zhao Yingxuan , Chen Depeng
Published: November 2019

Superhydrophobic surfaces have drawn attention from scientists and engineers because of their extreme water repellency. More interestingly, these surfaces have also demonstrated an infinite influence on civil engineering materials. In this feature article, the history of wettability theory is described firstly. The approaches to construct hierarchical micro/nanostructures such as chemical vapor deposition (CVD), electrochemical, etching, and flame synthesis methods are introduced. Then, the advantages and limitations of each method are discussed. Furthermore, the recent progress of superhydrophobicity applied on civil engineering materials and its applications are summarized. Finally, the obstacles and prospects of superhydrophobic civil engineering materials are stated and expected. This review should be of interest to scientists and civil engineers who are interested in superhydrophobic surfaces and novel civil engineering materials.
Characterization of Ceramics Coatings Processed by Sol-Gel for Cutting Tools
Authors: Rezende Bruna Aparecida , dos Santos Anderson Júnior , Câmara Marcelo Araújo , do Carmo Denilson José , Houmard Manuel , Rodrigues Alessandro Roger , Campos Rubio Juan Carlos
Published: November 2019

In order to obtain better cutting tool performance, the coatings appear as an alternative in the machining process. The goal of the coating is to improve tribological conditions in the chip-tool and tool-workpiece interfaces. On the other hand, the use of coated tools decreases the wear of the tools. This study discusses the ceramic coatings characterization deposited in WC tools. The Al2O3 and TiO2 films present properties such as thermal stability, chemical inertia, high hardness, and good mechanical properties. These coatings were prepared by sol-gel technology. The results indicated that the multilayer coating presents better adhesion on the substrate. Moreover, lower coefficients of friction were found for the coated tools. The analysis of variance (ANOVA) was used to evaluate the influence of the cutting parameters and tool coating on the cutting force. The lower cutting force was obtained using the multilayer-coated tool. Thus, the sol-gel method appears as a novel technique to deposit coating in the WC tools to improve their performance.
A Stochastic Model and Investigation into the Probability Distribution of the Thickness of Boride Layers Formed on Low-Carbon Steel
Authors: Velázquez-Altamirano Julio C. , Torres-Avila Itzel P. , Teran-Méndez Gerardo , Capula-Colindres Selene I. , Cabrera-Sierra Roman , Carrera-Espinoza Rafael , Hernández-Sánchez Enrique
Published: November 2019

The stochastic nature of the thickness of boride layers formed on carbon steel is described in this paper. Additionally, the probability distribution of the layer thickness is studied to determine the best-fit probability distribution. The study combines the use of an empirical model (power-law) and the Markov chain principles, with the purpose of demonstrating that it is feasible to develop a model that represents the non-uniformity of the thickness of boride layers that form on carbon steel. The results indicate that the mean and variance tend to increase when the time or temperature is increased. The findings of this paper demonstrate that an analytical solution to the Kolmogorov’s system differential equation can adequately represent the behavior of non-uniform boride layer formed on low-carbon steel, regardless of the temperature or duration of treatment.
A Newly Synthesized Heterobimetallic NiII-GdIII Salamo-BDC-Based Coordination Polymer: Structural Characterization, DFT Calculation, Fluorescent and Antibacterial Properties
Authors: Cui Yong-Fan , Zhang Yu , Xie Ke-Feng , Dong Wen-Kui
Published: November 2019

A unprecedented hetero-bimetallic 3d-4f BDC-salamo-based coordination polymer, [(L)Ni(BDC)Gd(NO3)(DMF)] was prepared and validated via elemental analyses, IR and UV–Visible absorption spectra, DFT calculation, and X-ray crystallography. The six-coordinated Ni1 ion lies at the N2O2 donor site of the L2− moiety, and one DMF O atom and carboxylate O atom occupy, collectively, the axial positions, and form a twisted octahedron. The nine-coordinated Gd1 ion consists of three oxygen atoms (O12, O13, and O14) of two carboxylate groups, two oxygen atoms (O8 and O9) derived from one bidentate nitrate group, and an O2O2 coordination site (O1, O2, O6, and O5) of the L2− unit, forming a twisted three-capped triangular prism coordination geometry. Compared to the ligand (H2L), the fluorescence intensity decreases due to the coordination of metal ions. Meanwhile, the antibacterial activities are researched.
Impact of Land Use on Bacterial Diversity and Community Structure in Temperate Pine and Indigenous Forest Soils
Authors: Amoo Adenike Eunice , Babalola Olubukola Oluranti
Published: November 2019

Soil microbial communities are an important part of ecosystems that possess the capability to improve ecosystem services; however, several aspects of the ecology of forest soil bacterial communities are still unknown. Here, we investigated the impact of land-use change on soil bacterial communities and the soil characteristics. High-throughput sequencing was used to ascertain the bacterial diversity and canonical correspondence analysis was used to determine relationships between the bacterial communities and environmental variables. Our results show spatial heterogeneity in the distribution of the microbial communities and significant relationships between the microbes and soil characteristics (axis 1 of the canonical correspondence analysis (CCA) plot explained 64.55% of the total variance while axis 2 described 24.49%). Knowledge of this is essential as it has direct consequences for the functioning of the soil ecosystem.
Transducer Electronic Data Sheets: Anywhere, Anytime, Anyway
Authors: Viegas Vítor , Postolache Octavian , Dias Pereira J.M.
Published: November 2019

Transducer electronic data sheets (TEDS) are a key element of smart transducers because they support core features such as plug and play, self-calibration, and self-diagnostics. The ISO/IEC/IEEE 21451-4 standard defines templates to describe the most common types of transducers and suggests the use of one-wire memories to store the corresponding data. In this paper we explore new ways to store and access TEDS tables, including near field communication (NFC) tags and QR codes. We also present a mobile TEDS parser, compatible with Android, that is capable of reading TEDS data from all supported mediums (one-wire memories, NFC tags, and QR codes) and decoding them as human-readable text. The idea is to make TEDS available in the easiest way possible. We also underline the need to extend the 21451-4 standard by adding support for frequency–time sensors. A new TEDS template is proposed, and filling examples are presented. The main novelties of the paper are (i) the proposal of new ways to store 21451-4 TEDS tables using NFC tags and QR codes; (ii) the release of new tools to access TEDS tables including a mobile parser; and (iii) the definition of a new TEDS template for frequency–time sensors.
Design and Analysis for Early Warning of Rotor UAV Based on Data-Driven DBN
Authors: Chen Xue-Mei , Wu Chun-Xue , Wu Yan , Xiong Nai-xue , Han Ren , Ju Bo-Bo , Zhang Sheng
Published: November 2019

The unmanned aerial vehicle (UAV), which is a typical multi-sensor closed-loop flight control system, has the properties of multivariable, time-varying, strong coupling, and nonlinearity. Therefore, it is very difficult to obtain an accurate mathematical diagnostic model based on the traditional model-based method; this paper proposes a UAV sensor diagnostic method based on data-driven methods, which greatly improves the reliability of the rotor UAV nonlinear flight control system and achieves early warning. In order to realize the rapid on-line fault detection of the rotor UAV flight system and solve the problems of over-fitting, limited generalization, and long training time in the traditional shallow neural network for sensor fault diagnosis, a comprehensive fault diagnosis method based on deep belief network (DBN) is proposed. Using the DBN to replace the shallow neural network, a large amount of off-line historical sample data obtained from the rotor UAV are trained to obtain the optimal DBN network parameters and complete the on-line intelligent diagnosis to achieve the goal of early warning as possible as quickly. In the end, the two common faults of the UAV sensor, namely the stuck fault and the constant deviation fault, are simulated and compared with the back propagation (BP) neural network model represented by the shallow neural network to verify the effectiveness of the proposed method in the paper.
The Application of Water Cycle Optimization Algorithm for Optimal Placement of Wind Turbines in Wind Farms
Authors: Rezk Hegazy , Fathy Ahmed , Diab Ahmed A. Zaki , Al-Dhaifallah Mujahed
Published: November 2019

Wind farms (WFs) include an enormous number of wind turbines (WTs) in order to achieve high capacity. The interaction among WTs reduces the extracted amount of wind energy because wind speed decreases in the wake region. The optimal placement of WTs within a WF is therefore vital for achieving high performance. This permits as many WTs as possible to be installed inside a narrow region. In this work, the water cycle algorithm (WCA), a recently developed optimizer, was employed to identify the optimal distribution of WTs. Minimization of the total cost per kilowatt was the objective of the optimization process. Two different cases were considered: the first assumed constant wind speed with variable wind direction, while the second applied variable wind speed with variable wind direction. The results obtained through the WCA optimizer were compared with other algorithms, namely, salp swarm algorithm (SSA), satin bowerbird optimization (SBO), grey wolf optimizer (GWO), and differential evolution (DE), as well as other reported works. WCA gave the best solution compared to other reported and programmed algorithms, thus confirming the reliability and validity of WCA in optimally configuring turbines in a wind farm for both the studied cases.
Experimental Study of Sensor Fault-Tolerant Control for an Electro-Hydraulic Actuator Based on a Robust Nonlinear Observer
Authors: Van Nguyen Tan , Ha Cheolkeun
Published: November 2019

Electro-hydraulic actuators (EHAs) have been widely used in modern industries. However, sensor faults and actuator faults in EHA systems can arise due to aging during operation, making the system unstable and unsafe. To solve these issues, fault-tolerant control (FTC) techniques for EHA systems have been studied intensively. In this paper, an FTC is proposed and developed for the mini motion package (MMP) EHA system. First, a mathematical model of the MMP system is formulated and improved to provide position tracking control using a well-known proportional-integral-derivative (PID) controller. Second, an unknown input observer (UIO) reconstruction is performed to estimate the states, disturbances, and sensor faults so that an asymptotically stable control error can be obtained by a linear matrix inequality (LMI) optimization algorithm through Lyapunov’s stability condition. Third, the FTC designed for the nonlinear discrete-time system is formed from fault compensation based on a residual logic signal to implement the fault compensation process and ensure stability and tracking performance with respect to minimizing impacts of disturbances and sensor faults. Here, residual is defined by the difference between state response and state estimation. Finally, numerical simulations and experiments of the MMP system are presented to illustrate the efficiency of the proposed FTC technique.
Analysis of Dense-Mesh Distribution Network Operation Using Long-Term Monitoring Data
Authors: Ptacek Michal , Vycital Vaclav , Toman Petr , Vaculik Jan
Published: November 2019

The technical and economic aspects and the possibility of the mesh network topology offering many radial configurations lead to the fact that large municipal networks are generally under radial operation. However, it is very important to analyze the operation and control of the mesh networks, especially in terms of their safety and durability and in the frame of the smart grid concept, respectively. The article deals with the analysis of the operation of the dense-mesh municipal distribution network of E.ON Distribuce a.s. based on the long-term data from power quality monitors. It also shows a brief view of the current lack of data usability from monitors installed in distribution networks in the context of smart grid.
The Relationship between Lifestyle Factors and Obesity Indices among Adolescents in Qatar
Authors: Kerkadi Abdelhamid , Sadig Abdelmonem H. , Bawadi Hiba , Al Thani Al Anoud Mohammed , Al Chetachi Walaa , Akram Hammad , Al-Hazzaa Hazzaa M. , Musaiger Abdulrahman O.
Published: November 2019

Background: Physical inactivity, sedentary behaviour and an unhealthy diet are factors that may increase weight and general and/or abdominal obesity. Objective: To evaluate the relationship between general and abdominal obesity and lifestyle factors among adolescents in Qatar. Methods: The study data are based on the Arab Teens Lifestyle Study (ATLS). The target population consisted of 1184 adolescents aged between 14 and 18 years old (563 boys and 621 girls), randomly selected through multistage sampling. A validated questionnaire was used to collect data on lifestyle indicators. Anthropometric indicators, which included body weight, height and waist circumference (WC), were measured according to standardised procedures. International Obesity Task Force (IOTF) age- and sex-specific body mass index (BMI) reference values were used to define overweight and obesity. Abdominal obesity was defined by the ‘waist-to-height ratio’ (WHtR > 0.5) and by sex- and age-specific WC cutoff values. Results: Females were more inactive than males (63.7% vs. 36.3%; p < 0.001). The proportion of adolescents who reported screen time of over 2 h per day was 82.5%. Females engaged in more sedentary behaviour than males (53.4% vs. 46.4%, p = 0.009). Being male (OR: 1.3; CI: 1.0–1.7) and skipping breakfast (OR: 1.5; CI: 1.2–2) were significantly associated with overweight/obesity. In contrast, high intake of fast food, fries, sweets and cake were negative predictors of general and abdominal obesity. Conclusions: The findings revealed the prevalence of unhealthy lifestyle habits among adolescents in Qatar and indicated relationships between certain dietary habits and obesity. The findings of this study may help in advocating for the implementation of an intervention that includes lifestyle changes targeting adolescents.
Effect of Sequence of Fruit Intake in a Meal on Satiety
Authors: Abdul Hakim Bibi Nabihah , Yahya Hanis Mastura , Shahar Suzana , Abdul Manaf Zahara , Damanhuri Hanafi
Published: November 2019

Little is known about the effects of manipulating sequence of fruit consumption during a meal in suppressing an individual’s appetite. Therefore, we investigate the effects of the sequence of fruit intake on satiety and blood glucose in a group of 17 healthy, young male adults. This intervention study repeatedly measured the effects of fruit intake (120 g red apple) before and after a meal and control (no fruit). Ad libitum test meal was weighed before and after a meal. Subjective appetite rating and appetite-related hormones were assessed at regular time intervals. The satiety score was significantly higher for fruit intake before a meal followed by after a meal and control (p < 0.05). Eating fruit before a meal reduced 18.5% (166 kcal) subsequent energy intake compared to control (p < 0.05). Fruit intake before a meal had a significantly higher incremental area under the curve (iAUC) of Glucagon-like peptide 1 (GLP-1), compared to after a meal (p < 0.05). There were no differences in plasma changes of ghrelin, Cholecystokinin 8 (CCK8), or blood glucose in all sessions. Consuming fruit before a meal potentially enhanced satiety. Further research is required to confirm both short- and long-term effects of the sequence of fruit intake on appetite regulation in a wider population.
Predictors of Counselling Related to a Healthy Lifestyle Carried Out by a General Practitioner
Authors: Znyk Małgorzata , Polańska Kinga , Wojtysiak Piotr , Szulc Michał , Bąk-Romaniszyn Leokadia , Makowiec-Dąbrowska Teresa , Zajdel-Całkowska Justyna , Kaleta Dorota
Published: November 2019

The aim of the study was to assess whether general practitioners (GPs) monitor and evaluate the health behavior of their patients in the field of a diet, physical activity, and weight control, and whether they provide appropriate counselling as part of this evaluation. Predictors of those activities among physicians were also determined. The cross-sectional study was conducted in the Piotrkowski district among 200 GPs. The questionnaire covered socio-demographic data and lifestyle characteristics of the physicians, their role as healthy lifestyle providers, and whether they assess lifestyle characteristics of their patients and perform healthy lifestyle counselling. More than 60% of the GPs did not evaluate lifestyle features during their patients’ examination. In total, 56% of the GPs provided healthy lifestyle recommendations among patients who have not been diagnosed with chronic lifestyle-related diseases but who do not follow healthy recommendations, and 73% of GPs provided recommendations to patients with chronic diseases related to lifestyle. The study showed that the chance to assess lifestyle characteristics of the patients was significantly higher for the GPs who believed that they were obliged to do so (OR = 6.5; p = 0.002). The chance to recommend a healthy lifestyle among patients who have not been diagnosed with chronic lifestyle-related diseases but who do not follow healthy recommendations was 5.9 times higher among the GPs working in the public sector (p < 0.001) and 16.3 times higher for these who believed that they had sufficient knowledge to provide the advice (p = 0.02). The following predictors of providing a healthy lifestyle counselling among patients with diagnosed chronic lifestyle-related diseases were identified: conviction that a GPs is obligated to provide it (OR = 4.4; p = 0.02), sufficient knowledge (OR = 8.7; p = 0.01), and following health recommendations by themselves (OR = 3.9; p = 0.04). Conclusions: The identified predictors are crucial for the development of appropriate strategies aiming at increasing GPs’ involvement in preventive measures and consequently at improving the population’s health.
Characteristics and Health Risk Assessment of Semi-Volatile Organic Contaminants in Rural Pond Water of Hebei Province
Authors: Liu Lin , Rao Zhu , Wang Yuan , Arandiyan Hamidreza , Gong Jie , Liang Ming , Guo Feng
Published: November 2019

Pond water as surface water has certain environmental impacts on environmental media such as groundwater, lakes, atmosphere, and soil. Organic pollutants present in pond water may pose health risks to humans, but research on organic pollutants in pond water is rare. Here, taking pond water collected in rural areas of Hebei province as the sample, we analyzed and evaluated four categories of semi-volatile organic compounds (SVOCs), including 11 phenolic compounds, 7 aniline compounds, 16 parent polycyclic aromatic hydrocarbons (PAHs), 14 PAH derivatives, and 16 phthalate esters (PAEs). The results show that the 10 water samples contained 26.2–17034 ng/L of Σ phenols, 33.7–2612 ng/L of Σ anilines, 33.9–1651 ng/L of Σ PAHs, and 59.0–2800 ng/L of Σ PAEs. Furthermore, non-carcinogenic risk and carcinogenic risk caused by SVOCs through direct ingestion and dermal exposure were also assessed. The current levels of non-carcinogenic risks and carcinogenic risks through these two means of exposure are within acceptable limits, except for the site 1 and site 5 in Hebei province where a total cancer risk exceeds 10−6. It can be concluded that the pond water studied had a low risk of carcinogenicity to the human.
Sleep Difficulties in Preschoolers with Psychiatric Diagnoses
Authors: Chénier-Leduc Gabrielle , Béliveau Marie-Julie , Dubois-Comtois Karine , Butler Bryan , Berthiaume Claude , Pennestri Marie-Hélène
Published: November 2019

Background: Sleep problems among preschoolers are highly prevalent. Given the impact of poor sleep quality on development, this relationship is particularly relevant in vulnerable populations but is less documented. This study aims to document parental perception of sleep problems in preschoolers assessed in a psychiatric clinic, as a function of diagnosis type. Methods: Children (14–71 months, n = 228) were evaluated by a psychiatrist, and diagnoses were pooled into four categories: behavioral disorders, relational disorders/psychosocial problems, developmental coordination disorder (DCD), and communication disorders. Sleep problems were measured using the Child Behavior Checklist (CBCL). Results: In this clinical sample of preschoolers, 21.6% of children were identified as having a sleep problem by their parents. Behavioral disorders and communication disorders were associated with increased parental report of sleep problems (respectively, trouble falling asleep and nighttime awakenings), while DCD was associated with lower parental report of sleep problems (fewer nighttime awakenings and less difficulty falling asleep) (p < 0.05). Relational disorders were not associated with parental reports of sleep difficulties (p > 0.05). Moreover, some psychiatric categories were associated with specific sleep symptoms (such as difficulty falling asleep and night awakenings). Conclusion: Parents of preschoolers with behavioral disorders and communication disorders are more likely to report sleep problems in their children than parents of preschoolers with DCD and relational disorders. Since different categories of psychiatric disorders are associated with specific types of sleep complaints, screening, and treatment should be adapted accordingly.
Real-World Gestational Diabetes Screening: Problems with the Oral Glucose Tolerance Test in Rural and Remote Australia
Authors: Jamieson Emma L. , Spry Erica P. , Kirke Andrew B. , Atkinson David N. , Marley Julia V.
Published: November 2019

Gestational diabetes mellitus (GDM) is the most common antenatal complication in Australia. All pregnant women are recommended for screening by 75 g oral glucose tolerance test (OGTT). As part of a study to improve screening, 694 women from 27 regional, rural and remote clinics were recruited from 2015–2018 into the Optimisation of Rural Clinical and Haematological Indicators for Diabetes in pregnancy (ORCHID) study. Most routine OGTT samples were analysed more than four hours post fasting collection (median 5.0 h, range 2.3 to 124 h), potentially reducing glucose levels due to glycolysis. In 2019, to assess pre-analytical plasma glucose (PG) instability over time, we evaluated alternative sample handling protocols in a sample of participants. Four extra samples were collected alongside routine room temperature (RT) fluoride-oxalate samples (FLOXRT): study FLOXRT; ice slurry (FLOXICE); RT fluoride-citrate-EDTA (FC Mix), and RT lithium-heparin plasma separation tubes (PST). Time course glucose measurements were then used to estimate glycolysis from ORCHID participants who completed routine OGTT after 24 weeks gestation (n = 501). Adjusting for glycolysis using FLOXICE measurements estimated 62% under-diagnosis of GDM (FLOXRT 10.8% v FLOXICE 28.5% (95% CI, 20.8–29.5%), p < 0.001). FC Mix tubes provided excellent glucose stability but gave slightly higher results (Fasting PG: +0.20 ± 0.05 mmol/L). While providing a realistic alternative to the impractical FLOXICE protocol, direct substitution of FC Mix tubes in clinical practice may require revision of GDM diagnostic thresholds.
Measuring SDG 15 at the County Scale: Localization and Practice of SDGs Indicators Based on Geospatial Information
Authors: Liu Shaoyang , Bai Jianjun , Chen Jun
Published: November 2019

To achieve the goal of worldwide sustainable protection and utilization of terrestrial ecosystems, it is necessary to quantitatively assess the implementation of Sustainable Development Goal 15 (SDG 15) at all administrative levels, especially at the grass-roots level, using the indicator framework of the UN SDGs. However, in the SDG 15 indicator system, the relationship between goal and indicators is ambiguous, and the results of the indicators cannot be visualized to show the differences within regions. Moreover, its design scale is country-oriented, which suggests that the indicator system cannot be applied directly to the county levels. In light of these issues, this paper used four modalities of localization to form an indicator system of localization, and applied it in the quantitative evaluation of meeting the objectives of SDG 15 in Deqing County, China. The localized indicator system for county level based on geospatial information included six indicators, which were clustered into three categories: sustainable forest management, halt and reverse land degradation, and conservation of biodiversity. By comparing and evaluating the quantitative results of SDG 15 in Deqing, 70% of the comparable indicators in the localization indicator system were at the forefront of those in China or the world. The results showed that grouped analysis of the targets and indicators could clarify the relationship between the implications of the goal and indicators, and the indicator system based on the geographic information was conducive to displaying the spatial distribution of the results of the indicators and clarifying the internal differences.
Conditional Deletion of Dicer in Adult Mice Impairs Skeletal Muscle Regeneration
Authors: Oikawa Satoshi , Lee Minjung , Akimoto Takayuki
Published: November 2019

Skeletal muscle has a remarkable regenerative capacity, which is orchestrated by multiple processes, including the proliferation, fusion, and differentiation of the resident stem cells in muscle. MicroRNAs (miRNAs) are small noncoding RNAs that mediate the translational repression or degradation of mRNA to regulate diverse biological functions. Previous studies have suggested that several miRNAs play important roles in myoblast proliferation and differentiation in vitro. However, their potential roles in skeletal muscle regeneration in vivo have not been fully established. In this study, we generated a mouse in which the Dicer gene, which encodes an enzyme essential in miRNA processing, was knocked out in a tamoxifen-inducible way (iDicer KO mouse) and determined its regenerative potential after cardiotoxin-induced acute muscle injury. Dicer mRNA expression was significantly reduced in the tibialis anterior muscle of the iDicer KO mice, whereas the expression of muscle-enriched miRNAs was only slightly reduced in the Dicer-deficient muscles. After cardiotoxin injection, the iDicer KO mice showed impaired muscle regeneration. We also demonstrated that the number of PAX7+ cells, cell proliferation, and the myogenic differentiation capacity of the primary myoblasts did not differ between the wild-type and the iDicer KO mice. Taken together, these data demonstrate that Dicer is a critical factor for muscle regeneration in vivo.
Alpha-Lipoic Acid Ameliorates Radiation-Induced Lacrimal Gland Injury through NFAT5-Dependent Signaling
Authors: Kim Hyuna , Yoo Woong-Sun , Jung Jung Hwa , Jeong Bae Kwon , Woo Seung Hoon , Kim Jin Hyun , Kim Seong Jae
Published: November 2019

Dry eye syndrome related to radiation therapy is relatively common and can severely impair a patient’s daily life. The nuclear factor of activated T cells 5(NFAT5) is well known for its osmoprotective effect under hyperosmolar conditions, and it also has immune-modulating functions. We investigated the role of NFAT5 and the protective effect of α-lipoic acid(ALA) on radiation-induced lacrimal gland (LG) injuries. Rats were assigned to control, ALA only, radiation only, and ALA administered prior to irradiation groups. The head and neck area, including the LG, was evenly irradiated with 2 Gy/minute using a photon 6-MV linear accelerator. NFAT5 expression was enhanced and localized in the LG tissue after irradiation and was related to cellular apoptosis. ALA had a protective effect on radiation-induced LG injury through the inhibition of NFAT5 expression and NFAT5-dependent signaling pathways. Functional radiation–induced damage of the LG and cornea was also restored with ALA treatment. NFAT5 expression and its dependent signaling pathways were deeply related to radiation-induced dry eye, and the condition was improved by ALA treatment. Our results suggest a potential role of NFAT5 and NF-κB in the proinflammatory effect in LGs and cornea, which offers a target for new therapies to treat dry eye syndrome.
Upgrading the Repertoire of miRNAs in Gastric Adenocarcinoma to Provide a New Resource for Biomarker Discovery
Authors: Pewarchuk Michelle E. , Barros-Filho Mateus C. , Minatel Brenda C. , Cohn David E. , Guisier Florian , Sage Adam P. , Marshall Erin A. , Stewart Greg L. , Rock Leigha D. , Garnis Cathie , Lam Wan L.
Published: November 2019

Recent studies have uncovered microRNAs (miRNAs) that have been overlooked in early genomic explorations, which show remarkable tissue- and context-specific expression. Here, we aim to identify and characterize previously unannotated miRNAs expressed in gastric adenocarcinoma (GA). Raw small RNA-sequencing data were analyzed using the miRMaster platform to predict and quantify previously unannotated miRNAs. A discovery cohort of 475 gastric samples (434 GA and 41 adjacent nonmalignant samples), collected by The Cancer Genome Atlas (TCGA), were evaluated. Candidate miRNAs were similarly assessed in an independent cohort of 25 gastric samples. We discovered 170 previously unannotated miRNA candidates expressed in gastric tissues. The expression of these novel miRNAs was highly specific to the gastric samples, 143 of which were significantly deregulated between tumor and nonmalignant contexts (p-adjusted < 0.05; fold change > 1.5). Multivariate survival analyses showed that the combined expression of one previously annotated miRNA and two novel miRNA candidates was significantly predictive of patient outcome. Further, the expression of these three miRNAs was able to stratify patients into three distinct prognostic groups (p = 0.00003). These novel miRNAs were also present in the independent cohort (43 sequences detected in both cohorts). Our findings uncover novel miRNA transcripts in gastric tissues that may have implications in the biology and management of gastric adenocarcinoma.
Gene Therapy in Retinal Dystrophies
Authors: Ziccardi Lucia , Cordeddu Viviana , Gaddini Lucia , Matteucci Andrea , Parravano Mariacristina , Malchiodi-Albedi Fiorella , Varano Monica
Published: November 2019

Inherited retinal dystrophies (IRDs) are a group of clinically and genetically heterogeneous degenerative disorders. To date, mutations have been associated with IRDs in over 270 disease genes, but molecular diagnosis still remains elusive in about a third of cases. The methodologic developments in genome sequencing techniques that we have witnessed in this last decade have represented a turning point not only in diagnosis and prognosis but, above all, in the identification of new therapeutic perspectives. The discovery of new disease genes and pathogenetic mechanisms underlying IRDs has laid the groundwork for gene therapy approaches. Several clinical trials are ongoing, and the recent approval of Luxturna, the first gene therapy product for Leber congenital amaurosis, marks the beginning of a new era. Due to its anatomical and functional characteristics, the retina is the organ of choice for gene therapy, although there are quite a few difficulties in the translational approaches from preclinical models to humans. In the first part of this review, an overview of the current knowledge on methodological issues and future perspectives of gene therapy applied to IRDs is discussed; in the second part, the state of the art of clinical trials on the gene therapy approach in IRDs is illustrated.
Family Perspectives on Newborn Screening for X-Linked Adrenoleukodystrophy in California
Authors: Schwan Katharina , Youngblom Janey , Weisiger Kara , Kianmahd Jessica , Waggoner Rebecca , Fanos Joanna
Published: November 2019

X-linked adrenoleukodystrophy (ALD) is caused by gene variants in the ABCD1 gene, resulting in a varied clinical spectrum. Males with ALD present with symptoms ranging from isolated adrenal insufficiency and slowly progressive myelopathy to severe cerebral demyelination. Females who are heterozygous for ALD typically develop milder symptoms by late adulthood. Treatment for adrenal insufficiency associated with ALD exists in the form of cortisol, and cerebral ALD may be treated with stem cell transplantation. Currently, there is no treatment for myelopathy. Since 2013, at least 14 states have added ALD to their newborn screening (NBS) panel, including California in 2016. We examined the impact of a positive NBS result for ALD on families in California. Qualitative interviews were conducted with mothers of 10 children who were identified via NBS for ALD. Interviews were transcribed verbatim and analyzed using thematic analysis by two coders. Mothers felt strongly that ALD should be included on California’s NBS panel; however, many expressed concerns over their experience. Themes included stress at initial phone call, difficulty living with uncertainty, concerns regarding mental health support, and desire for more information on disease progression, treatments and clinical trials. Mothers exhibited diverse coping strategies, including relying on faith, information seeking, and maintaining hope. Mothers’ recommendations for healthcare providers included: educating providers making the initial phone call, providing patient-friendly resources, offering information about ongoing research, and streamlining care coordination. Advice for parents of children with ALD focused on staying hopeful and appreciating the time they have with their children. As more states add ALD to their NBS panel, it is important to improve the current model to promote family resiliency and autonomy.