bluuebunny/crossref_metadata_2025 huggingface dataset:
A versatile high throughput strategy for cloning the env gene of HIV-1
Authors: Mishra Nitesh , Dobhal Ayushman , Sharma Shaifali , Luthra Kalpana
Published: April 2020

The trimeric envelope glycoprotein (gp120/gp41)3of human immunodeficiency virus-1 (HIV-1) mediates viral and host cell membrane fusion, initiated by binding of viral envelope gp120 protein to the CD4 receptor on host immune cells. Functional env genes from infected individuals have been widely used as templates for vaccine design, for setting up viral neutralization assays and to study the viral evolution and pathogenesis. Traditional topoisomerase or T4 DNA polymerase mediated approaches for cloning single genome amplified (SGA) env genes are labor-intensive, cost-ineffective with low-throughput, thereby enabling functional analysis of only a limited number of env genes from the diverse circulating quasispecies in infected individuals. Herein, we report an efficient, easy to optimize and high-throughput approach for cloning diverse HIV-1 env genes. Multiple env/rev gene cassettes, derived from infected infants, were subjected to SGA using Phusion polymerase and utilized as megaprimers in overlap extension PCR mediated cloning (OEC), circumventing the requirement for novel enzymes. Furthermore, utilization of Phusion polymerase for both the amplification of env/rev cassettes and OEC allows convenient monitoring and optimization, thereby providing much greater flexibility and versatility for analysis of env genes from HIV-1 infected individuals.
Accumulation of mutations in genes associated with sexual reproduction contributed to the domestication of a vegetatively propagated staple crop, enset
Authors: Tesfamicael Kiflu Gebramicael , Gebre Endale , March Timothy J , Sznajder Beata , Mather Diane E. , Marcelino Rodriguez Lopez Carlos
Published: April 2020

Enset (Ensete ventricosum(Welw.) Cheesman) is a drought tolerant, vegetatively propagated crop that was domesticated in Ethiopia. It is a staple food for more than 20 million people in Ethiopia. Despite its current importance and immense potential, enset is among the most genetically understudied and underexploited food crops. We collected 230 enset wild and cultivated accessions across the main enset producing regions in Ethiopia and applied amplified fragment length polymorphism and genotype by sequencing (GBS) methods to these accessions. Wild and cultivated accessions were clearly separated from each other, with 89 genes found to harbour SNPs that separated wild from cultivated accessions. Among these, 17 genes are thought to be involved in flower initiation and seed development. Among cultivated accessions, differentiation was mostly associated with geographical location and with proximity to wild populations. Our results indicate that vegetative propagation of elite clones has favoured capacity for vegetative growth at the expense of capacity for sexual reproduction. This is consistent with previous reports that cultivated enset tends to produce non-viable seeds and flowers less frequent than wild enset.
Intervention Serology and Interaction Substitution: Modeling the Role of ‘Shield Immunity’ in Reducing COVID-19 Epidemic Spread
Authors: Weitz Joshua S. , Beckett Stephen J. , Coenen Ashley R. , Demory David , Dominguez-Mirazo Marian , Dushoff Jonathan , Leung Chung-Yin , Li Guanlin , Măgălie Andreea , Park Sang Woo , Rodriguez-Gonzalez Rogelio , Shivam Shashwat , Zhao Conan
Published: April 2020

The COVID-19 pandemic has precipitated a global crisis, with more than 690,000 confirmed cases and more than 33,000 confirmed deaths globally as of March 30, 2020 [1–4]. At present two central public health control strategies have emerged: mitigation and suppression (e.g, [5]). Both strategies focus on reducing new infections by reducing interactions (and both raise questions of sustainability and long-term tactics). Complementary to those approaches, here we develop and analyze an epidemiological intervention model that leverages serological tests [6, 7] to identify and deploy recovered individuals as focal points for sustaining safer interactions via interaction substitution, i.e., to develop what we term ‘shield immunity’ at the population scale. Recovered individuals, in the present context, represent those who have developed protective, antibodies to SARS-CoV-2 and are no longer shedding virus [8]. The objective of a shield immunity strategy is to help sustain the interactions necessary for the functioning of essential goods and services (including but not limited to tending to the elderly [9], hospital care, schools, and food supply) while decreasing the probability of transmission during such essential interactions. We show that a shield immunity approach may significantly reduce the length and reduce the overall burden of an outbreak, and can work synergistically with social distancing. The present model highlights the value of serological testing as part of intervention strategies, in addition to its well recognized roles in estimating prevalence [10, 11] and in the potential development of plasma-based therapies [12–15].
KnetMiner: a comprehensive approach for supporting evidence-based gene discovery and complex trait analysis across species
Authors: Hassani-Pak Keywan , Singh Ajit , Brandizi Marco , Hearnshaw Joseph , Amberkar Sandeep , Phillips Andrew L. , Doonan John H. , Rawlings Chris
Published: April 2020

Generating new ideas and scientific hypotheses is often the result of extensive literature and database reviews, overlaid with scientists’ own novel data and a creative process of making connections that were not made before. We have developed a comprehensive approach to guide this technically challenging data integration task and to make knowledge discovery and hypotheses generation easier for plant and crop researchers. KnetMiner can digest large volumes of scientific literature and biological research to find and visualise links between the genetic and biological properties of complex traits and diseases. Here we report the main design principles behind KnetMiner and provide use cases for mining public datasets to identify unknown links between traits such grain colour and pre-harvest sprouting inTriticum aestivum, as well as, an evidence-based approach to identify candidate genes under anArabidopsis thalianapetal size QTL. We have developed KnetMiner knowledge graphs and applications for a range of species including plants, crops and pathogens. KnetMiner is the first open-source gene discovery platform that can leverage genome-scale knowledge graphs, generate evidence-based biological networks and be deployed for any species with a sequenced genome. KnetMiner is available athttp://knetminer.org.
In-depth characterization of layer 5 output neurons of the primary somatosensory cortex innervating the mouse dorsal spinal cord
Authors: Frezel N. , Platonova E. , Voigt F.F. , Mateos J.M. , Kastli R. , Ziegler U. , Karayannis T. , Helmchen F. , Wildner H. , Zeilhofer H.U.
Published: April 2020

Neuronal circuits of the spinal dorsal horn integrate sensory information from the periphery with inhibitory and facilitating input from higher CNS areas. Most previous work focused on projections descending from the hindbrain. Less is known about inputs descending from the cerebral cortex. Here, we identified cholecystokinin (CCK) positive layer 5 pyramidal neurons of the primary somatosensory cortex (CCK+S1-CST neurons) as a major source of input to the spinal dorsal horn. We combined intersectional genetics and virus-mediated gene transfer to characterize CCK+S1-CST neurons and to define their presynaptic input and postsynaptic target neurons. We found that S1-CST neurons constitute a heterogeneous population that can be subdivided into distinct molecular subgroups. Rabies-based retrograde tracing revealed monosynaptic input from layer 2/3 pyramidal neurons, from parvalbumin (PV) positive cortical interneurons, and from thalamic relay neurons in the ventral posterolateral nucleus. WGA-based anterograde tracing identified postsynaptic target neurons in dorsal horn laminae III and IV. About 60% of these neurons were inhibitory and about 60% of all spinal target neurons expressed the transcription factor c-Maf. The heterogeneous nature of both S1-CST neurons and their spinal targets suggest complex roles in the fine-tuning of sensory processing.
Expression of a transcript encoding for cytosolic renin in the diseased human heart
Authors: Sczodrok Jaroslaw , Lutze Philipp , Staar Doreen , Dörr Marcus , Peters Jörg
Published: April 2020

Secretory renin promotes hypertrophy, apoptosis, necrosis and fibrosis through angiotensin generation. It has been claimed that local expression of renin contributes to the deleterious effects of the renin-angiotensin system in the heart. Besides the classic renin transcript (renin-a), encoding for secretory renin, a putative brain-specific (renin-b) and a putative lung-specific (renin-c) transcript may exist in human. In contrast to secretory renin, renin-b cannot be secreted, remains within the cytosol and is imported into mitochondria. In contradiction to renin-a, renin-b exerts cardioprotective effects in hearts and in cardiac cells of the rat under ischemia related conditions. To date, available data on cardiac renin expression remain inconsistent. Nobody has yet investigated which renin transcripts are expressed in the human heart. We systematically analyzed the levels of renin transcripts using specific and sensitive nested reverse-transcriptase polymerase chain reactions (RT-PCR) in human ventricular biopsies obtained from patients with heart diseases. In the 33 biopsies available, neither the expression of classic renin-a, nor of the alternative renin-c was detected. In contrast, the renin-b transcript, which was previously classified as brain-specific, was found in 11/33 ventricular biopsies. Our data exclude the expression of secretory renin and indicate that local expression of cytosolic renin but not of secretory renin can play a functional role in the human heart.
Evaluation of DNA conservation in Nile-Saharan environment, Missiminia, in Nubia: Tracking maternal lineage of “X-Group”
Authors: Cherifi Yahia Mehdi Seddik , Amrani Selma
Published: April 2020

Objective

We assessed DNA conservation using a range of archaeological skeletal samples from Sudan (Missiminia in Upper Nubia, 350 B.C.E to 1400 C.E) from the unfavorable conditions of the Saharan milieu and humidity of the Nile valley by tracking maternal lineage on the ‘X-Group’ (Ballaneans).

Method

We were able to extract, amplify, and sequence mt-DNA HVS-I (Sanger sequencing method) from 11 petrous bone samples, eight for the X-Group set and three for the reference set (one Christian, one Late Meroitic, and one Meroitic).

Results

It was possible to find the haplogroups (L1b, L2, L3, H2, N, T1a, X and W) and to carry out comparative data analysis in relation to haplogroup data cited in the literature. This investigation into the maternal lineage of X-Group (350 to 500 C.E.) origins allowed us to validate the efficiency of petrous bone sampling from ancient human remains from the Nile-Saharan milieu and established that the Ballaneans experienced an in-situ development with more admixture from the Levant region and North Africa.

Conclusions

Our study used mt-DNA (HVS-I) to look for the biological origins of the X-Group from Upper-Nubia and demonstrated the feasibility of ancient DNA research on skeletons from the Nile-Saharan environment. The use of Next Sequencing Generation (NGS) should optimize and improve the detection of shorter DNA strands and their sequencing in complete genomes from ancient skeletal remains (petrous bones) from hot and humid environments.
Private Genomes and Public SNPs: Homomorphic encryption of genotypes and phenotypes for shared quantitative genetics
Authors: Mott Richard , Fischer Christian , Prins Pjotr , Davies Robert William
Published: April 2020

Sharing human genotype and phenotype data presents a challenge because of privacy concerns, but is essential in order to discover otherwise inaccessible genetic associations. Here we present a method of homomorphic encryption that obscures individuals’ genotypes and phenotypes and is suited to quantitative genetic association analysis. Encrypted ciphertext and unencrypted plaintext are interchangeable from an analytical perspective. This allows one to store ciphertext on public web services and share data across multiple studies, while maintaining privacy. The encryption method uses as its key a high-dimensional random linear orthogonal transformation that leaves the likelihood of quantitative trait data unchanged under a linear model with normally distributed errors. It also preserves linkage disequilibrium between genetic variants and associations between variants and phenotypes. It scrambles relationships between individuals: encrypted genotype dosages closely resemble Gaussian deviates, and in fact can be replaced by quantiles from a Gaussian with only negligible effects on accuracy. Standard likelihood-based inferences are unaffected by orthogonal encryption. These include the use of mixed linear models to control for unequal relatedness between individuals, the estimation of heritability, and the inclusion of covariates when testing for association. Orthogonal transformations can also be applied in a modular fashion that permits multi-party federated mega-analyses. Under this scheme any number of parties first agree to share a common set of genotype sites and covariates prior to encryption. Each party then privately encrypts and shares their own ciphertext, and analyses the other parties’ ciphertexts. In the absence of private variants, or knowledge of the key, we show that it is infeasible to decrypt ciphertext using existing brute-force or noise reduction attacks. Therefore, we present the method as a challenge to the community to determine its security.
People can learn new walking patterns without walking
Authors: Song Christine N. , Stenum Jan , Leech Kristan A. , Keller Chloe , Roemmich Ryan T.
Published: April 2020

Humans can learn many new walking patterns. People have learned to snowshoe up mountains, racewalk marathons, and march in precise synchrony. But what is required to learn a new walking pattern? Here, we demonstrate that people can learn new walking patterns without actually walking. Through a series of experiments, we observe that stepping with only one leg can facilitate learning of an entirely new walking pattern (i.e., split-belt treadmill walking). We find that the nervous system learns from the relative motion between the legs – whether or not both legs are moving – and can transfer this learning to novel gaits. We also show that locomotor learning requires active movement: observing another person adapt their gait did not result in significantly faster learning. These findings reveal that people can learn new walking patterns without bilateral gait training, as stepping with one leg can facilitate adaptive learning that transfers to novel gait patterns.
Mutant EGFR is a preferred SMURF2 substrate of ubiquitination: role in enhanced receptor stability and TKI sensitivity
Authors: Ray Paramita , Raghunathan Krishnan , Ahsan Aarif , Allam Uday Sankar , Shukla Shirish , Basrur Venkatesha , Veatch Sarah , Lawrence Theodore S. , Nyati Mukesh K. , Ray Dipankar
Published: April 2020

We previously reported that differential protein degradation of TKI-sensitive [L858R, del(E746-A750)] and resistant (T790M) epidermal growth factor receptor (EGFR) mutants upon erlotinib treatment correlates with drug sensitivity. However, the molecular mechanism remains unclear. We also reported SMAD ubiquitination regulatory factor 2 (SMURF2) ligase activity is important in stabilizing EGFR. Here, usingin vitroandin vivoubiquitination assays, mass spectrometry, and super-resolution microscopy, we show SMURF2-EGFR functional interaction is critical in receptor stability and TKI sensitivity. We found that L858R/T790M EGFR is a preferred substrate of SMURF2-UBCH5 (an E3-E2) complex-mediated K63-linked polyubiquitination, which preferentially stabilizes mutant receptor. We identified four lysine (K) residues (K721, 846, 1037 and 1164) as the sites of ubiquitination and replacement of K to acetylation-mimicking asparagine (Q) at K1037 position in L858R/T790M background converts the stable protein sensitive to erlotinib-induced degradation. Using STochastic Optical Reconstruction Microscopy (STORM) imaging, we show that SMURF2 presence allows longer membrane retention of activated EGFR upon EGF treatment, whereas, siRNA-mediatedSMURF2knockdown fastens receptor endocytosis and lysosome enrichment. In an erlotinib-sensitive PC9 cells, SMURF2 overexpression increased EGFR levels with improved erlotinib tolerance, whereas,SMURF2knockdown decreased EGFR steady state levels in NCI-H1975 and PC9-AR cells to overcome erlotinib and AZD-9291 resistance respectively. Additionally, by genetically altering the SMURF2-UBCH5 complex formation destabilized EGFR. Together, we propose that SMURF2-mediated preferential polyubiquitination of L858R/T790M EGFR may be competing with acetylation-mediated receptor internalization to provide enhanced receptor stability and that disruption of the E3-E2 complex may be an attractive alternate to overcome TKI resistance.
miTAR: a hybrid deep learning-based approach for predicting miRNA targets
Authors: Gu Tongjun , Zhao Xiwu , Barbazuk William Bradley , Lee Ji-Hyun
Published: April 2020

microRNAs (miRNAs) are a major type of small RNA that alter gene expression at the post-transcriptional or translational level. They have been shown to play important roles in a wide range of biological processes. Many computational methods have been developed to predict targets of miRNAs in order to understand miRNAs’ function. However, the majority of the methods depend on a set of pre-defined features that require considerable effort and resources to compute, and these methods often do not effectively on the prediction of miRNA targets. Therefore, we developed a novel hybrid deep learning-based approach that is capable to predict miRNA targets at a higher accuracy. Our approach integrates two deep learning methods: convolutional neural networks (CNNs) that excel in learning spatial features, and recurrent neural networks (RNNs) that discern sequential features. By combining CNNs and RNNs, our approach has the advantages of learning both the intrinsic spatial and sequential features of miRNA:target. The inputs for the approach are raw sequences of miRNA and gene sequences. Data from two latest miRNA target prediction studies were used in our study: the DeepMirTar dataset and the miRAW dataset. Two models were obtained by training on the two datasets separately. The models achieved a higher accuracy than the methods developed in the previous studies: 0.9787 vs. 0.9348 for the DeepMirTar dataset; 0.9649 vs. 0.935 for the miRAW dataset. We also calculated a series of model evaluation metrics including sensitivity, specificity, F-score and Brier Score. Our approach consistently outperformed the current methods. In addition, we compared our approach with earlier developed deep learning methods, resulting in an overall better performance. Lastly, a unified model for both datasets was developed with an accuracy higher than the current methods (0.9545). We named the unified model miTAR for miRNA target prediction. The source code and executable are available athttps://github.com/tjgu/miTAR.
Experience-independent transformation of single-cell 3D genome structure and transcriptome during postnatal development of the mammalian brain
Authors: Tan Longzhi , Ma Wenping , Wu Honggui , Zheng Yinghui , Xing Dong , Chen Ritchie , Li Xiang , Daley Nicholas , Deisseroth Karl , Xie X. Sunney
Published: April 2020

Both transcription and 3D organization of the mammalian genome play critical roles in neurodevelopment and its disorders. However, 3D genome structures of single brain cells have not been solved; little is known about the dynamics of single-cell transcriptome and 3D genome after birth. Here we generate a transcriptome atlas of 3,517 cells and a 3D genome atlas of 3,646 cells from the developing mouse cortex and hippocampus, using our high-resolution MALBAC-DT and Dip-C methods. In adults, 3D genome “structure types” delineate all major cell types, with high correlation between A/B compartments and gene expression. During development, both transcriptome and 3D genome are extensively transformed in the first postnatal month. In neurons, 3D genome is rewired across multiple scales, correlated with gene expression modules and independent of sensory experience. Finally, we examine allele-specific structure of imprinted genes, revealing local and chromosome-wide differences. These findings uncover a previously unknown dimension of neurodevelopment.

HIGHLIGHTS

Transcriptomes and 3D genome structures of single brain cells (both neurons and glia) in the developing mouse forebrain

Cell type identity encoded in the 3D wiring of the mammalian genome (“structure types”)

Major transformation of both transcriptome and 3D genome during the first month of life, independent of sensory experience

Allele-specific 3D structure at 7 imprinted gene loci, including one that spans a whole chromosome
Disinhibition of the orbitofrontal cortex biases decision making in obesity
Authors: Seabrook Lauren T. , Naef Lindsay , Baimel Corey , Judge Allap K. , Kenney Tyra , Ellis Madelyn , Tayyab Temoor , Armstrong Mataea , Qiao Min , Floresco Stan B , Borgland Stephanie L.
Published: April 2020

The lateral orbitofrontal cortex (lOFC) receives sensory information about food and integrates these signals with expected outcomes to guide future actions, and thus may play a key role in a distributed network of neural circuits that regulate feeding behaviour. Here, we reveal a novel role for the lOFC in the cognitive control of behaviour in obesity. Food-seeking behaviour is biased in obesity such that in male obese mice, behaviours are less flexible to changes in the perceived value of the outcome. Obesity is associated with reduced lOFC inhibitory drive, and chemogenetic reduction in GABAergic neurotransmission in the lOFC induces obesity-like impairments in goal-directed behaviour. Conversely, pharmacological or optogenetic restoration of inhibitory neurotransmission in the lOFC of obese mice reinstates flexible behaviour. Our results indicate that obesity-induced disinhibition of the lOFC leads to a failure to update changes in the value of food with satiety, which in turn may influence how individuals make decisions in an obesogenic environment.
STEP inhibition prevents Aβ-mediated damage in dendritic complexity and spine density in Alzheimer’s disease.
Authors: Chatterjee Manavi , Kwon Jeemin , Benedict Jessie , Kamceva Marija , Kurup Pradeep , Lombroso Paul J.
Published: April 2020

Loss of dendritic spines and decline of cognitive function are hallmarks of patients with Alzheimer’s disease (AD). Previous studies have shown that AD pathophysiology involves increased expression of a central nervous system-enriched protein tyrosine phosphatase called STEP (STriatal-Enriched protein tyrosine Phosphatase). STEP opposes the development of synaptic strengthening by dephosphorylating substrates, including GluN2B, Pyk2 and ERK1/2. Genetic reduction of STEP as well as pharmacological inhibition of STEP improves cognitive function and hippocampal memory in the 3xTg AD mouse model. Here, we show that the improved cognitive function is accompanied by an increase in synaptic connectivity in cell cultures as well as in the triple transgenic AD mouse model, further highlighting the potential of STEP inhibitors as a therapeutic agent.
What do women know about breast density? A public screening program perspective
Authors: Robertson Monique , Darcey Ellie C , Dench Evenda K , Keogh Louise , McLean Kirsty , Pirikahu Sarah , Saunders Christobel , Thompson Sandra , Woulfe Catherine , Wylie Elizabeth , Stone Jennifer
Published: April 2020

Background

This study assesses knowledge of breast density, one of breast cancer’s strongest risk factors, in women attending a public mammographic screening program in Western Australia that routinely notifies women if they have dense breasts.

Methods

Survey data was collected from women who were notified they have dense breasts and women who had not (controls). Descriptive data analysis was used to summarize responses.

Results

Of the 6183 women surveyed, over 85% of notified women knew that breast density makes it difficult to see cancer on a mammogram (53.9% in controls). A quarter of notified women knew that having dense breasts puts women at increased risk for breast cancer (13.2% in controls). Overall, 50.1% of notified women indicated that they thought the amount of information provided was “just right” and 24.9% thought it was “too little”, particularly women notified for the first time (32.1%).

Conclusion

The main message of reduced sensitivity of mammography in women with dense breasts provided by the screening program appears to be getting though. However, women are largely unaware that increased breast density is associated with increased risk. Women notified of having dense breasts for the first time could potentially benefit from additional information.
Alpha oscillations do not implement gain control in early visual cortex but rather gating in parieto-occipital regions
Authors: Zhigalov Alexander , Jensen Ole
Published: April 2020

Spatial attention provides a mechanism for respectively enhancing relevant and suppressing irrelevant information. While it is well-established that attention modulates oscillations in the alpha band, it remains unclear if alpha oscillations are involved in directly modulating the neuronal excitability associated with the allocation of spatial attention. In this study in humans, we utilized a novel broadband frequency (60 – 70 Hz) tagging paradigm to quantify neuronal excitability in relation to alpha oscillations in a spatial attention paradigm. We used magnetoencephalography to characterize ongoing brain activity as it allows for localizing the sources of both the alpha and frequency tagging responses. We found that attentional modulation of alpha power and the frequency tagging response are uncorrelated over trials. Importantly, the neuronal sources of the tagging response were localized in early visual cortex (V1) whereas the sources of the alpha activity were identified around parieto-occipital sulcus. Moreover, we found that attention did not modulate the latency of the frequency tagged responses. Our findings point to alpha band oscillations serving a downstream gating role rather than implementing gain control of excitability in early visual regions.

Significance Statement

By combining magnetoencephalography and a novel broadband frequency tagging approach, we show that spatial attention differently modulates alpha oscillations and neuronal excitability. Importantly, the sources of the alpha oscillations and tagging responses were spatially distinct and the alpha power and tagging response were not related over trials. These results are inconsistent with previous ideas suggesting that alpha oscillations are involved in gain control of early sensory regions; rather alpha oscillations are involved in the allocation of neuronal resources in downstream regions.
Dehydration synthesis and crystal structure of terbium oxychloride, TbOCl
Authors: Chong Saehwa , Riley Brian J. , Nelson Zayne J.
Published: April 2020

Terbium oxychloride, TbOCl, was synthesizedviathe simple heat-treatment of TbCl3·6H2O and its structure was determined by refinement against X-ray powder diffraction data. TbOCl crystallizes with the matlockite (PbFCl) structure in the tetragonal space groupP4/nmmand is composed of alternating (001) layers of (TbO)nandnCl−. The unit-cell parameters, unit-cell volume, and density were compared to the literature data of other isostructural rare-earth oxychlorides in the same space group and showed good agreement when compared to the calculated trendlines.
Ni3Te2O2(PO4)2(OH)4, an open-framework structure isotypic with Co3Te2O2(PO4)2(OH)4
Authors: Eder Felix , Weil Matthias
Published: April 2020

Single crystals of Ni3(TeO(OH)2)2(PO4)2, trinickel(II) bis[(oxidodihydoxidotellurate(IV)] bis(phosphate),were obtained by hydrothermal synthesis at 483 K, starting from NiCO3·2Ni(OH)2, TeO2 and H3PO4 in a molar ratio of 1:2:2. The crystal structure of Ni3Te2O2(PO4)2(OH)4 is isotypic with that of Co3Te2O2(PO4)2(OH)4 [Zimmermann et al. (2011). J. Solid State Chem. 184, 3080–3084]. The asymmetric unit comprises two Ni (site symmetries \overline{1}, 2/m) one Te (m), one P (m), five O (three m, two 1) and one H (1) sites. The tellurium(IV) atom shows a coordination number of five, with the corresponding [TeO3(OH)2] polyhedron having a distorted square-pyramidal shape. The two NiII atoms are both octahedrally coordinated but form different structural elements: one constitutes chains made up from edge-sharing [NiO6] octahedra extending parallel to [010], and the other isolated [NiO2(OH)4] octahedra. The two kinds of nickel/oxygen octahedra are connected by the [TeO3(OH)2] pyramids and the [PO4] tetrahedra through edge- and corner-sharing into a three-dimensional framework structure with channels extending parallel to [010]. Hydrogen bonds of medium strength between the hydroxy groups and one of the phosphate O atoms consolidate the packing. A quantitative structure comparison between Ni3Te2O2(PO4)2(OH)4 and Co3Te2O2(PO4)2(OH)4 is made.
The effect of servant leadership, perceived organizational support, job satisfaction and job embeddedness on turnover intentions
Authors: Huning Tobias M. , Hurt Kevin J. , Frieder Rachel E.
Published: April 2020

Purpose

The purpose of this study is to provide insights into the effect of servant leadership on turnover intentions. The authors investigate the mediating effects of perceived organizational support (POS), job embeddedness and job satisfaction on the relationship between servant leadership and turnover intentions. In doing so, the authors seek to make the following contributions. First, the authors seek to provide additional empirical evidence for servant leadership as an effective organizational theory. Additionally, the authors seek to establish POS, embeddedness and job satisfaction as underlying mechanisms that transmit the positive effects of servant leadership.

Design/methodology/approach

The data were collected from a paper and pencil survey questionnaire provided to employees of different organizations in a metropolitan area in the southeastern United States. The sample consisted of 150 participants; complete (listwise) data were available for 115 participants.

Findings

The study shows that POS and embeddedness are mediating mechanisms through which servant leadership is related to employee turnover intentions. The authors found POS and job embeddedness to be significant mediating constructs which help explain the nature of the relationship between servant leadership and turnover intentions.

Originality/value

By investigating these constructs in the present framework, we help to provide answers to the questions of how and why servant leadership affects employee outcomes. These answers are an important step towards more fully understanding the complex ways by which followers respond to servant leadership.
The effect of complete versus partial observations on service evaluations
Authors: Abbasi Marjan
Published: April 2020

Purpose

The purpose of this paper is to investigate the effect of complete versus partial observations of service failure and recovery. This study also aims at investigating the effect of observing customers’ need for cognitive closure and types of compensation that a service provider offers.

Design/methodology/approach

Two experiments are conducted to test the research hypotheses. The authors use scenarios describing failure and recovery encounters that occur to a target customer at restaurant settings, and through manipulation of complete versus partial observations, they investigate observers’ attitudes and behavioral intentions.

Findings

The results suggest that customers with a partial observation are less forgiving than those with a complete observation. In particular, the former sympathized more with a target customer, blamed a service provider more and a target customer less and had lower repurchase intentions than the latter. The authors find that the need for cognitive closure heightens this tendency following a partial observation of service failure. They also find that following a complete (versus partial) observation, observers reacted more favorably to service recovery when it included (versus did not include) monetary compensation.

Research limitations/implications

This research studies the effect of locus of causality following a partial versus complete observation. Future research could further examine the effect of stability and controllability. Also, the authors examined the effect of the need for cognitive closure on evaluations of service failure following a partial versus complete observation. Future research could examine the effect of some other individual difference variables.

Practical implications

The results offer some measures to be taken by practitioners. In particular, practitioners are advised to not offer monetary compensation when majority of observers have had a partial observation. Moreover, they are advised to offer some explanation in a timely and effective manner to ensure observers who are under the negative impact of a partial observation have some information so that they revisit their service evaluations.

Originality/value

The literature assumes that in failure and recovery incidents, all observing customers would know the entire story. This research challenges this assumption and highlights the key role of observation type (partial versus complete observation). Further, this research examines the effect of the need for cognitive closure on service evaluations following a partial versus complete observation. The current research finds that supposedly favorable measures by a firm (i.e. monetary compensation) may in fact backfire when a partial observation is at play.
Barriers and facilitators to pap-testing among female overseas Filipino workers: a qualitative exploration
Authors: Christie-de Jong Floor , Reilly Siobhan
Published: April 2020

Purpose

Every year, 311,000 women die of cervical cancer globally, a disease which is preventable and treatable. Pap-testing should be part of a comprehensive approach to tackling cervical cancer; however, barriers to pap-testing do exist and migration may present additional barriers. In 2018, 2.3 million overseas Filipino workers were recorded and uptake of pap-testing for this group is low. The study aims to understand barriers and enablers to screening for overseas Filipino workers, which is essential to improve uptake of pap-testing for this population.

Design/methodology/approach

Embedded in a mixed-methods study, an exploratory qualitative study was conducted with Web-based, in-depth interviews (N = 8) with female overseas Filipino workers, mostly domestic workers, based in Kuwait, Qatar, Singapore and Hong Kong. Results were analysed using thematic analysis. A socio-ecological conceptual framework was used to explore barriers to uptake of pap-testing.

Findings

Barriers to pap-testing were cognitive factors, such as limited knowledge and fear of the outcome of pap-testing, as well as cultural and structural barriers. Findings revealed structural contexts not conducive to pap-testing, including difficulty navigating the health-care system, poverty, difficult employment circumstances and the overriding need to provide financially for family and children in the Philippines.

Originality/value

This study explored barriers to pap-testing with a hard-to-reach group, who are underrepresented in the literature. Barriers to pap-testing were embedded in structural barriers, resulting in health inequalities. Host and sending countries benefit from overseas Filipino workers and have a responsibility to care for their health and well-being, and should strive to tackle these structural factors.
Executive compensation and corporate social responsibility: does a golden parachute matter?
Authors: Okafor Collins E. , Ujah Nacasius U.
Published: April 2020

Purpose

This study examines the efficacy of compensation in encouraging corporate executives to promote corporate social responsibility (CSR). In particular, it closely examines the effect of a golden parachute (GP) on an executive's behavior toward CSR.

Design/methodology/approach

This study uses longitudinal data on 1,301 US firms for the period from 1993 to 2013. The data comes from Compustat, MSCI ESG STATS, RiskMetrics and ExecuComp.

Findings

We find an inverse association between current and long-term compensations and GP on firms' CSR. However, a test on the moderating effect discloses that a GP and long-term compensation jointly and positively increase the firms' CSR performance. This increase supports the idea that executives with a GP seek to maximize their long-term wealth by approving CSR projects that add value. The results also show that female executives are more likely to promote CSR than their male counterparts, and older executives are less willing to engage in CSR projects.

Practical implications

Adding a GP contractual clause to the executive compensation package could encourage greater engagement in CSR projects. The CEO with a GP will ensure that the firm engages in only value-enhancing CSR projects; this should align the interest of the society (greater firm engagement in CSR) with the interest of the firm (value maximization).

Originality/value

This study contributes to the literature by examining the moderating effect of a GP on the association between CSR and executive compensation.
Choosing high-equity cosmetic brands in bad macroeconomic conditions: evidence from panel data
Authors: Kaswengi Joseph , Diallo Mbaye Fall , Akrout Houcine , Valette-Florence Pierre
Published: April 2020

Purpose

This study investigates how price, promotion and consumer characteristics affect consumer choice of high over medium- and low-equity cosmetic brand under different macroeconomic conditions.

Design/methodology/approach

The study uses purchase records from MarketingScan's Behaviour Scan panels (a GFK – Mediametrie Company) covering the period from 2008 to 2009. The panel analysed represents a sample of 2,149 households representative of the national population.

Findings

Results indicate that regular price and relative brand price increase high-equity cosmetic brand choice over both low- and medium-equity brands, while reference price decreases it. Brand feature promotion activity and joint promotion positively affect high-equity cosmetic brand choice, whereas display promotion decreases it. In comparison to medium-equity cosmetic brands, gender and education slightly increase high-equity cosmetic brand choice, while age decreases it. Surprisingly, household income does not affect high-equity cosmetic brand choice. The effect of regular price decreases over worsening macroeconomic conditions. However, the effect of relative brand price decreases between low and moderate contraction periods, but increases between moderate and high contraction times. Feature promotion is effective only when the contraction is moderate, while the negative effect of display promotion is stable over time.

Originality/value

The paper underlines the moderating role of macroeconomic conditions on the relationship between pricing decisions as well as promotion activity and consumer choice of high-equity cosmetic brands.
Get employees talking through enterprise social media! Reduce cyberslacking: a moderated mediation model
Authors: Nivedhitha K.S. , Sheik Manzoor A.K.
Published: April 2020

Purpose

While the sizable body of research focusses on various psychological effects of enterprise social media (ESM), research connecting the link between ESM and cyberslacking is still very sparse. Drawing inspiration from the social bonding theory, the authors have proposed a moderated mediation model to explain how ESM affordances reduce cyberslacking, mediated significantly by workplace social bonding when the perceived co-worker involvement is high.

Design/methodology/approach

Utilising a sample of 384 respondents, the study tested the model fit and hypothesised relationships with AMOS and PROCESS MACRO.

Findings

The findings show that workplace social bonding mediates the relationship between ESM affordances and cyberslacking. The results are also consistent with the moderated mediation model as the mediation is significant when the perceived co-worker involvement is high, and the mediation is insignificant when the moderator is low/moderate.

Research limitations/implications

Considering the potential behavioural dynamics of ESM artefacts, the authors have introduced self-expression (via microblogging), recognition (from paralinguistic digital affordances) and network externality as ESM affordances. Alongside the existing measures to reduce employee deviance, the proposed model with the above-mentioned affordances can be investigated in detail by the future research community.

Practical implications

In light of the findings, the study demonstrates that ESM can be treated as a mechanism to keep cyberslacking at bay. The results offer significant implications for managers, who lookout for innovative and soft strategies to reduce cyberslacking in the workplace.

Originality/value

Instead of implementing strict policies to kerb cyberslacking, this study proposes an alternative and an interesting model by introducing ESM as a strategic tool in reducing cyberslacking. The paper argues that ESM, being a potential tool for employee engagement and bonding, may offset the employees' tendency to involve in cyberslacking.
Role of technological dimensions of green supply chain management practices on firm performance
Authors: Bag Surajit , Gupta Shivam , Kumar Sameer , Sivarajah Uthayasankar
Published: April 2020

Purpose

The research study aims to investigate green supply chain management (GSCM) elements as part of a complete system. It aims to understand the special properties of the GSCM system under the moderating effects of product complexity and purchasing structure.

Design/methodology/approach

A thorough literature review led to the building of the conceptual framework. Six constructs were identified using systems theory. These constructs include green supply chain technological dimensions (particularly, Artificial Intelligence (AI) based), green supply chain strategy, green supply chain process, product complexity, purchasing structure, and firm performance. The instrument was scientifically developed for gathering survey responses using complete design test methods. The conceptual model was eventually tested based on survey data collected from 250 automotive components and allied manufacturers in the emerging economy of South Africa.

Findings

The results indicate that GSCM technological dimensions (AI-based) positively influence GSCM strategy. Further, GSCM strategy was found to positively influence the GSCM process. The GSCM processes have significant effects on environmental performance, social performance, and financial performance. The product complexity has a significant moderation effect on the paths GSCM strategy and GSCM process.

Originality/value

The findings from multivariate data analysis provide a better understanding of GSCM system dynamics and are helpful to key decision-makers. This unique model has elevated GSCM theory to a new level. There are limited studies available in the existing GSCM literature using systems theory. This study will offer an advanced/comprehensive understanding to readers in this relatively new concept.
Emotional branding on fashion brand websites: harnessing the Pleasure-Arousal-Dominance (P-A-D) model
Authors: Yang Kiseol , Kim HaeJung Maria , Zimmerman Jonelle
Published: April 2020

Purpose

Drawn from the P-A-D approach, the purposes of this study are (1) to investigate the impacts of website attributes on emotional branding process, and (2) to examine the mediating effects of three affective dimensions of pleasure, arousal and dominance on behavioral response towards the fashion brand website.

Design/methodology/approach

By employing the quantitative approach of survey method, data were collected in the US and a sample of 220 participants was used for the analysis. Structural equation modeling analysis was conducted to identify hypothesized relationships in the research model.

Findings

Fashion brand website attributes were found to influence consumer affective experiential states and behavioral responses on emotional branding. Specifically, the mediating effects of the dominance and arousal dimensions were of interest to predict the pleasure dimension, leading to a positive response towards the brand. Positing arousal and dominance as predictors of the degree of pleasure on brand website were accentuated in emotional branding.

Originality/value

This study signifies the role of affect in the emotional branding on websites. Given the academic and practical significance of emotional branding on websites, the findings of the current study provide insights into fashion brands through making possible a fuller understanding of the underlying process of building emotional branding and of how to embed it into consumers' minds when they experience the fashion brand website.
Lean management and occupational health: team learning as a key factor
Authors: Stimec Arnaud
Published: April 2020

Purpose

Liker (2003) asserts that continuous improvement’s goal is to favour organisational learning (OL), the latter being one of the key principles of lean management (LM). Yet, OL may result in better occupational health (Panari et al., 2010). The purpose of this paper is to consider Liker’s assertion seriously and to study the relations between LM, team learning (TL) and occupational health.

Design/methodology/approach

For this purpose, the authors compared 22 teams in nine case studies. A mixed methodology approach called qualitative comparative analysis (Ragin, 1987) was used to test the hypothesis that TL plays a key role in preserving occupational health in LM implementations, leading to improved performance that is not at the expense of workers’ health.

Findings

The central hypothesis questioned was that complete LM when resulting in a TL dynamic (i.e. systemic lean), would enable both increases in industrial performance and maintain or improve occupational health. The data tend to validate this hypothesis.

Originality/value

This research may enrich three types of research literature that hardly know each other, namely, LM literature (production research), occupational health literature and OL literature. For professionals concerned with the social dimension of modern production, this research shows that there is a path for a mutual gain perspective in LM, but this is a demanding process with many conditions to satisfy.