bluuebunny/crossref_metadata_2025 huggingface dataset:
Citrus aurantium L. and Its Flavonoids Regulate TNBS-Induced Inflammatory Bowel Disease through Anti-Inflammation and Suppressing Isolated Jejunum Contraction
Authors: He Wei , Li Yongmin , Liu Mengyang , Yu Haiyang , Chen Qian , Chen Yue , Ruan Jingya , Ding Zhijuan , Zhang Yi , Wang Tao
Published: October 2018

Inflammatory bowel disease (IBD) is a serious digestive system disease, for which the clinical therapeutic choices remain limited. Dried fruits of Citrus aurantium L. (CAL) are a traditional medicine used for regulation of the digestive system. The aim of this study was to identify the regulatory effects of CAL on IBD and to clarify the mechanism of the active compounds. In trinitrobenzene sulfonic acid-induced IBD rats, 125 to 500 mg/kg of oral CAL significantly alleviated weight loss and diarrhea, decreased colitis inflammatory cell infiltration, and inhibited pro-inflammatory cytokine production. The mechanisms of characteristic flavonoids in CAL were evaluated involving inflammation and intestine contraction aspects. Naringenin, nobiletin, and hesperetin showed anti-inflammatory effects on lipopolysaccharide-induced RAW cells. The mechanism may be related to the inhibition of the tumor necrosis factor-α (TNF-α)-induced nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway to suppress cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expressions. Naringenin and nobiletin showed inhibitory effects on isolated jejunum contraction. The mechanism of naringenin is partly related to COX, NOS, inositol triphosphate (IP3), and finally, to decreased jejunum motility. This study demonstrated that CAL, and its flavonoids’ regulatory effects on IBD through anti-inflammation and inhibition of intestine muscle contraction, can provide basic information on developing new drugs or supplements against IBD based on CAL.
Granulin A Synergizes with Cisplatin to Inhibit the Growth of Human Hepatocellular Carcinoma
Authors: Qiao Gan , Xu Huanli , Li Cong , Li Xiao , Farooqi Ammad , Zhao Yuming , Liu Xiaohui , Liu Ming , Stagos Dimitrios , Lin Xiukun
Published: October 2018

Cisplatin is one of the most potent chemotherapy drugs widely used for cancer treatment. However, due to resistance and toxicity, the application of cisplatin for the treatment of hepatocellular carcinoma (HCC) is limited. Our previous study has shown that granulin A (GRN A), an anticancer peptide, is able to interact with enolase1 (ENO1) and inhibit the growth of HCC in vitro. In the present study, we studied the synergistic effect of the combination of cisplatin and GRN A for the inhibitory effect on HCC. An 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and Chou-Talalay approaches revealed that the combination of GRN A and cisplatin displayed potent synergistic effect. The colony formation and cell viability of HCC cells were inhibited significantly in cells treated with the combination of cisplatin and GRN A, compared with cells treated with cisplatin or GRN A alone. Overexpression of ENO1 diminished the synergistic effect of GRN A and cisplatin in HCC cells. The combination of the two drugs exhibited a more obvious inhibitory effect on cancer cell apoptosis, as analyzed by the cytometry flow, mitochondrial membrane potential (MMP) and western blot analysis. An in vivo study confirmed that the combined use of the two drugs displayed more potent antitumor activity compared to mice treated with cisplatin and GRN A alone; the inhibitory rate of tumor growth was 65.46% and 68.94%, respectively, in mice treated with GRN A and cisplatin. However, the inhibitory rate increased to 86.63% in mice treated with the combination of the two drugs. This study provides evidence that the combination of GRN A and cisplatin is able to sensitize the liver cancer to cisplatin, and that targeting ENO1 is a promising approach for enhancing the antitumor activity of cisplatin.
Roles of Gut-Derived Secretory Factors in the Pathogenesis of Non-Alcoholic Fatty Liver Disease and Their Possible Clinical Applications
Authors: Okubo Hirofumi , Kushiyama Akifumi , Nakatsu Yusuke , Yamamotoya Takeshi , Matsunaga Yasuka , Fujishiro Midori , Sakoda Hideyuki , Ohno Haruya , Yoneda Masayasu , Asano Tomoichiro
Published: October 2018

The rising prevalence of non-alcoholic fatty liver disease (NAFLD) parallels the global increase in the number of people diagnosed with obesity and metabolic syndrome. The gut-liver axis (GLA) plays an important role in the pathogenesis of NAFLD/non-alcoholic steatohepatitis (NASH). In this review, we discuss the clinical significance and underlying mechanisms of action of gut-derived secretory factors in NAFLD/NASH, focusing on recent human studies. Several studies have identified potential causal associations between gut-derived secretory factors and NAFLD/NASH, as well as the underlying mechanisms. The effects of gut-derived hormone-associated drugs, such as glucagon-like peptide-1 analog and recombinant variant of fibroblast growth factor 19, and other new treatment strategies for NAFLD/NASH have also been reported. A growing body of evidence highlights the role of GLA in the pathogenesis of NAFLD/NASH. Larger and longitudinal studies as well as translational research are expected to provide additional insights into the role of gut-derived secretory factors in the pathogenesis of NAFLD/NASH, possibly providing novel markers and therapeutic targets in patients with NAFLD/NASH.
Differential and Overlapping Effects of 20,23(OH)2D3 and 1,25(OH)2D3 on Gene Expression in Human Epidermal Keratinocytes: Identification of AhR as an Alternative Receptor for 20,23(OH)2D3
Authors: Slominski Andrzej T. , Kim Tae-Kang , Janjetovic Zorica , Brożyna Anna A. , Żmijewski Michal A. , Xu Hui , Sutter Thomas R. , Tuckey Robert C. , Jetten Anton M. , Crossman David K.
Published: October 2018

A novel pathway of vitamin D activation by CYP11A has previously been elucidated. To define the mechanism of action of its major dihydroxy-products, we tested the divergence and overlap between the gene expression profiles of human epidermal keratinocytes treated with either CYP11A1-derived 20,23(OH)2D3 or classical 1,25(OH)2D3. Both secosteroids have significant chemical similarity with the only differences being the positions of the hydroxyl groups. mRNA was isolated and examined by microarray analysis using Illumina’s HumanWG-6 chip/arrays and subsequent bioinformatics analyses. Marked differences in the up- and downregulated genes were observed between 1,25(OH)2D3- and 20,23(OH)2D3-treated cells. Hierarchical clustering identified both distinct, opposite and common (overlapping) gene expression patterns. CYP24A1 was a common gene strongly activated by both compounds, a finding confirmed by qPCR. Ingenuity pathway analysis identified VDR/RXR signaling as the top canonical pathway induced by 1,25(OH)2D3. In contrast, the top canonical pathway induced by 20,23(OH)2D3 was AhR, with VDR/RXR being the second nuclear receptor signaling pathway identified. QPCR analyses validated the former finding by revealing that 20,23(OH)2D3 stimulated CYP1A1 and CYP1B1 gene expression, effects located downstream of AhR. Similar stimulation was observed with 20(OH)D3, the precursor to 20,23(OH)2D3, as well as with its downstream metabolite, 17,20,23(OH)3D3. Using a Human AhR Reporter Assay System we showed marked activation of AhR activity by 20,23(OH)2D3, with weaker stimulation by 20(OH)D3. Finally, molecular modeling using an AhR LBD model predicted vitamin D3 hydroxyderivatives to be good ligands for this receptor. Thus, our microarray, qPCR, functional studies and molecular modeling indicate that AhR is the major receptor target for 20,23(OH)2D3, opening an exciting area of investigation on the interaction of different vitamin D3-hydroxyderivatives with AhR and the subsequent downstream activation of signal transduction pathways in a cell-type-dependent manner.
Synthesis and Structural Characterization of Ba7Li11Bi10 and AE4(Li,Tr)7Pn6 (AE = Sr, Ba, Eu; Tr = Ga, In; Pn = Sb, Bi)
Authors: Ojwang Dickson O. , Bobev Svilen
Published: October 2018

Reported are the synthesis and crystal structure of Ba7Li11Bi10, a new ternary compound crystallizing in its own type with the monoclinic space group C2/m (a = 18.407(3) Å, b = 5.0258(9) Å, and c = 18.353(3) Å; β = 104.43(1)°; Pearson symbol mS56), and those of the structurally related quaternary phases Ba4(Li1−xGax)7Sb6, Ba4(Li1−xInx)7Sb6, Ba4(Li1−xInx)7Bi6, and Eu4(Li1−xInx)7Bi6 (crystallizing in the Eu4Li7Bi6 structure type with the same monoclinic space group C2/m (a = 18.4045(13)–17.642(4) Å, b = 5.012(4)–4.8297(10) Å, and c = 13.2792(10)–12.850(3) Å, β = 126.80(1)–125.85(1)°; Pearson symbol mS34). All studied compounds are identified among the products of the high-temperature reactions of the corresponding elements. Both types of crystal structures are based on corner- and edge-linked Li-centered Sb4 (or Bi4) tetrahedra, Sb6 (or Bi6) octahedra, and Sb2 or Bi2 dumbbells. Given the similarities between the two structures, it might be proposed that they represent the simplest members of a potentially large homologous series described with the general formulae (BaLi3Sb2)n(Ba3Li4Sb4)m or (BaLi3Bi2)n(Ba3Li4Bi4)m, where the more complicated “7-11-10” phase is the member with n = 2 and m = 1, while the “4-7-6” one is the intergrowth of the two components in an equal ratio. The computed electronic band structures of Ba7Li11Bi10 and idealized Ba4Li7Bi6 (a model for Ba4(Li1−xInx)7Bi6) are also discussed.
Effects of Low Pressure Treatment on the Mortality of Different Life Stages of Indianmeal moth (Plodia interpunctella) and the Quality of Dried Chinese Jujube
Authors: Hou Juncai , Zhao Lushuai , Wang Shaojin
Published: October 2018

Low pressure technology is a potential non-chemical method to control insects in agriculture products. The purpose of this study was to determine the tolerance of different life stages of indianmeal moth (Plodia interpunctella) to low pressure and to validate the mortality of P. interpunctella when infesting Chinese jujube (Zizyphus jujuba Miller) using a low pressure system. Results showed that larvae were the most tolerant life stage of P. interpunctella, with a minimum exposure time of 41.4 h at 1.3 kPa and 25 °C to obtain 100% mortality. Pupae were the most susceptible life stage, with a lethal time of 12 h under the above low pressure conditions. The survival ratio of P. interpunctella in jujube decreased with increasing exposure time and reached 0% when the jujubes with P. interpunctella larvae were exposed to low-pressure treatment for 41.6 h at 1.3 kPa and 25 °C. Although the color, moisture, soluble solid, and vitamin C contents were slightly changed, there was no significant quality difference in these factors between control and treated jujubes. The information provided by this study is useful in developing effective non-chemical low-pressure treatments for disinfesting agricultural products.
Calf Circumference as a Screening Tool for Cognitive Frailty in Community-Dwelling Older Adults: The Korean Frailty and Aging Cohort Study (KFACS)
Authors: Kim Miji , Jeong Min Jeong , Yoo Jinho , Song Da Young , Won Chang Won
Published: October 2018

The aim of this study was to examine calf circumference in relation to cognitive frailty in community-dwelling older adults. Cross-sectional analysis was performed on the first-year baseline data of 1559 adults aged 70–84 years enrolled in the Korean Frailty and Aging Cohort Study. The final analysis included 1221 adults who were non-dependent in terms of instrumental activities of daily living, who underwent frailty and cognitive function assessments. Physical frailty was defined using the Fried Frailty Index. Cognitive impairment was defined as a score 1.5 standard deviations below the age-, sex- and education-matched norms on any of four cognitive-function tests. The prevalence of cognitive frailty was 2.8% for men and 3.8% for women. After adjusting for potential confounders, in comparison to the “physically robust without cognitive impairment” group, the estimates of increased odds ratios (ORs) for low calf circumference (<32 cm) were much greater in the prefrail with cognitive impairment (OR 4.62, 95% confidence interval (CI): 2.02–10.61) and frail with cognitive impairment (OR 10.94, 95% CI: 2.87–41.68) groups in men but not in women. Low calf circumference was strongly related to cognitive frailty in men only, suggesting calf circumference can be used as an indicator of these outcomes.
Thermal Buckling Behaviour of Thin and Thick Variable-Stiffness Panels
Authors: Vescovini Riccardo , Dozio Lorenzo
Published: October 2018

The possibility of designing composite panels with non-uniform stiffness properties offers a chance for achieving highly-efficient configurations. This is particularly true for buckling-prone structures, whose response can be shaped through a proper distribution of the membrane and bending stiffnesses. The thermal buckling behaviour of composite panels is among the aspects that could largely benefit from the adoption of a variable-stiffness design, but, in spite of that, it has rarely been addressed. The paper illustrates a semi-analytical approach for evaluating the thermal buckling response of variable-stiffness plates (VSP) by considering different boundary conditions. The formulation relies upon the method of Ritz and a variable-kinematic approach, leading to a computationally efficient implementation, which is particularly useful for exploring the larger design spaces, typical of variable-stiffness configurations. Due to the possibility of choosing the underlying kinematic approach as an input of the analysis, the formulation is not restricted to thin plates, but is suitable for analyzing the response of thick plates as well. Novel results are derived, which can be useful for benchmarking purposes and for gathering insight into the mechanical behaviour of variable-stiffness plates. Furthermore, the importance of transverse shear flexibility is illustrated with respect to the boundary conditions as well as the degree of steering of the fibers.
A Non-Structural Representation Scheme for Articulated Shapes
Authors: Genctav Asli , Tari Sibel
Published: October 2018

Articulated shapes are successfully represented by structural representations which are organized in the form of graphs of shape components. We present an alternative representation scheme which is equally powerful but does not require explicit modeling or discovery of structural relations. The key element in our scheme is a novel multi scale pixel-based distinctness measure which implicitly quantifies how rare a particular pixel is in terms of its geometry with respect to all pixels of the shape. The spatial distribution of the distinctness yields a partitioning of the shape into a set of regions. The proposed representation is a collection of size normalized probability distribution of the distinctness over regions over shape dependent scales. We test the proposed representation on a clustering task.
ECRU: An Encoder-Decoder Based Convolution Neural Network (CNN) for Road-Scene Understanding
Authors: Yasrab Robail
Published: October 2018

This research presents the idea of a novel fully-Convolutional Neural Network (CNN)-based model for probabilistic pixel-wise segmentation, titled Encoder-decoder-based CNN for Road-Scene Understanding (ECRU). Lately, scene understanding has become an evolving research area, and semantic segmentation is the most recent method for visual recognition. Among vision-based smart systems, the driving assistance system turns out to be a much preferred research topic. The proposed model is an encoder-decoder that performs pixel-wise class predictions. The encoder network is composed of a VGG-19 layer model, while the decoder network uses 16 upsampling and deconvolution units. The encoder of the network has a very flexible architecture that can be altered and trained for any size and resolution of images. The decoder network upsamples and maps the low-resolution encoder’s features. Consequently, there is a substantial reduction in the trainable parameters, as the network recycles the encoder’s pooling indices for pixel-wise classification and segmentation. The proposed model is intended to offer a simplified CNN model with less overhead and higher performance. The network is trained and tested on the famous road scenes dataset CamVid and offers outstanding outcomes in comparison to similar early approaches like FCN and VGG16 in terms of performance vs. trainable parameters.
Assessment of Suitability of Tree Species for Bioenergy Production on Burned and Degraded Peatlands in Central Kalimantan, Indonesia
Authors: Maimunah Siti , Rahman Syed Ajijur , Samsudin Yusuf B. , Artati Yustina , Simamora Trifosa Iin , Andini Sarah , Lee Soo Min , Baral Himlal
Published: October 2018

Large areas of deforested and degraded land, particularly degraded peatlands, need a viable long-term solution for restoration, ideally one that ensures energy security without compromising food security or biodiversity conversation. To address a knowledge gap on the most adaptive bioenergy crop(s) for degraded lands, this research project assessed the survival and growth performance of potential bioenergy crops to restore burned and degraded peatlands. Our methodology compared the bioenergy species with the potential to survive in extreme environments, i.e., gamal [Gliricidia sepium (Jacq.) Walp.], kaliandra (Calliandra calothyrsus Meissner), kemiri sunan [Reutealis trisperma (Blanco) Airy Shaw], and nyamplung (Calophyllum inophyllum L.). Observed parameters are plant survival rates, tree height, and circular stem growth. The experiment was conducted between March 2016 to February 2017 in a two-hectare demonstration plot on burned and degraded peatland in Buntoi village, Pulang Pisau, Central Kalimantan province. Using a split plot design, two treatments were given to each species, i.e., monoculture plantation and agroforestry (intercropped with Ananas comosus (L.) Merr.); with each treatment, the species were replicated on two separate plots. Results indicate that nyamplung is the most adoptable species followed by kemiri sunan, however both species performed very well under agroforestry treatment when compared with monoculture. Further study is needed to assess the productivity and associate biofuel yield.
Testing New Concepts for Crop Cultivation in Space: Effects of Rooting Volume and Nitrogen Availability
Authors: Wolff Silje A. , Palma Carolina F. , Marcelis Leo , Kittang Jost Ann-Iren , Van Delden Sander H.
Published: October 2018

Long term human missions to the Moon and Mars, rely on life support systems for food production and regeneration of resources. In the EU H2020 TIME SCALE-project, an advanced life support system concept was developed to facilitate plant research and technology demonstration under different gravity conditions. Ground experiments assessed irrigation systems and effects of rooting- and nutrient solution volume. The maximal allowed volume for existing International Space Station research facilities (3.4 L) was able to support cultivation of two lettuce heads for at least 24 days. A smaller rooting volume (0.6 L) increased root biomass after 24 days, but induced a 5% reduction in total biomass at day 35. Regulating effects of nitrate supply on plant water fluxes in light and dark were also investigated. At low concentrations of nitrate in the nutrient solution, both transpiration and stomatal conductance increased rapidly with increasing nitrate concentration. During day-time this increase levelled off at high concentrations, while during nigh-time there was a distinct decline at supra optimal concentrations. Plants supplied with nitrate concentrations as low as 1.25 mM did not show visible signs of nutrient stress or growth reduction. These findings hold promise for both reducing the environmental impact of terrestrial horticulture and avoiding nutrient stress in small scale closed cultivation systems for space.
Electrochemical Measurements of Multiwalled Carbon Nanotubes under Different Plasma Treatments
Authors: Abdul Rahim Zulaiha , Yusof Nor Azah , Mohammad Haniff Muhammad Aniq Shazni , Mohammad Faruq , Syono Mohd Ismahadi , Daud Nurulhaidah
Published: October 2018

In the present work, we described the post-treatment effects of applying different plasma atmosphere conditions on the electrochemical performances of the multiwalled carbon nanotubes (MWCNTs). For the study, a composite of MWCNTs/Co/Ti was successfully grown on the silicon substrate and then pre-treated with ammonia, oxygen and hydrogen plasma. The composite was characterized by making use of field emission scanning electron microscopy (FESEM) for the surface morphology and Raman spectroscopy for the functionalization. Further, the electrochemical measurements were performed with the use of the cyclic voltammetry (CV) applied in the 0.01 M potassium ferricyanide in 0.1 M KCl solution. On testing, the results indicated that the NH3-treated MWCNTs have the highest efficiency as compared to the other pretreatments and control. This increased performance of NH3 treated sample can be linked to the enhanced surface area of the composite, thereby improved adsorption and associated interaction with that of the analyte molecules at the electrodes. Further comparison of the electrode with that of commercial Dropsens electrodes provided the confirmation for the efficiency of the NH3/MWCNTs, thereby suggesting for the potentiality of applying the NH3 modified electrode towards electrochemical applications.
Grain Refinement by Second Phase Particles under Applied Stress in ZK60 Mg Alloy with Y through Phase Field Simulation
Authors: Song Yuhao , Wang Mingtao , Zong Yaping , He Ri , Jin Jianfeng
Published: October 2018

Based on the principle of grain refinement caused by the second-phase particles, a phase field model was built to describe the recrystallization process in the ZK60 alloy system with Y added under applied stress between temperatures 573 and 673 K for 140 min duration. The simulation of grain growth with second phase particles and applied stress during annealing process on industrial scale on the condition of real time-space was achieved. Quantitative analysis was carried out and some useful laws were revealed in ZK60 alloy system. The second phase particles had a promoting effect on the grain refinement, however the effect weakened significantly when the content exceeded 1.5%. Our simulation results reveal the existence of a critical range of second phase particle size of 0.3–0.4 μm, within which a microstructure of fine grains can be obtained. Applied stress increased the grain coarsening rate significantly when the stress was more than 135 MPa. The critical size of the second phase particles was 0.4–0.75 μm when the applied stress was 135 MPa. Finally, a microstructure with a grain size of 11.8–13.8 μm on average could be obtained when the second phase particles had a content of 1.5% and a size of 0.4–0.75 μm with an applied stress less than 135 Mpa after 30 min annealing at 573 K.
Cyclopropane-Containing Fatty Acids from the Marine Bacterium Labrenzia sp. 011 with Antimicrobial and GPR84 Activity
Authors: Amiri Moghaddam Jamshid , Dávila-Céspedes Antonio , Kehraus Stefan , Crüsemann Max , Köse Meryem , Müller Christa E. , König Gabriele Maria
Published: October 2018

Bacteria of the family Rhodobacteraceae are widespread in marine environments and known to colonize surfaces, such as those of e.g., oysters and shells. The marine bacterium Labrenzia sp. 011 is here investigated and it was found to produce two cyclopropane-containing medium-chain fatty acids (1, 2), which inhibit the growth of a range of bacteria and fungi, most effectively that of a causative agent of Roseovarius oyster disease (ROD), Pseudoroseovarius crassostreae DSM 16950. Additionally, compound 2 acts as a potent partial, β-arrestin-biased agonist at the medium-chain fatty acid-activated orphan G-protein coupled receptor GPR84, which is highly expressed on immune cells. The genome of Labrenzia sp. 011 was sequenced and bioinformatically compared with those of other Labrenzia spp. This analysis revealed several cyclopropane fatty acid synthases (CFAS) conserved in all Labrenzia strains analyzed and a putative gene cluster encoding for two distinct CFASs is proposed as the biosynthetic origin of 1 and 2.
Recovery of Cerium from Glass Polishing Waste: A Critical Review
Authors: Borra Chenna Rao , Vlugt Thijs J. H. , Yang Yongxiang , Offerman S. Erik
Published: October 2018

Ceria is the main component in glass polishing powders due to its special physico-chemical properties. Glass polishing powder loses its polishing ability gradually during usage due to the accumulation of other compounds on the polishing powder or due to changes in the particle size distribution. The recovery of cerium from the glass polishing waste results in the efficient utilization of natural resources. This paper reviews processes for the recovery of rare earths from polishing waste. Glass polishing powder waste can be reused via physical, physico-chemical or chemical processes by removing silica and/or alumina. The removal of silica and/or alumina only improves the life span up to some extent. Therefore, removal of other elements by chemical processes is required to recover a cerium or cerium-rich product. However, cerium leaching from the polishing waste is challenging due to the difficulties associated with the dissolution of ceria. Therefore, high acid concentrations, high temperatures or costly reducing agents are required for cerium dissolution. After leaching, cerium can be extracted from the leach solution by solvent extraction or selective precipitation. The product can be used either in glass polishing again or other high value added applications.
Study of Nd Electrodeposition from the Aprotic Organic Solvent Dimethyl Sulfoxide
Authors: Bourbos Evangelos , Karantonis Antonis , Sygellou Labrini , Paspaliaris Ioannis , Panias Dimitrios
Published: October 2018

The use of organic solvents in an electrolytic system for neodymium electrorecovery by electrolysis at low temperatures is studied in the current work. More specifically, an alternative route, that of the system of DMSO (Dimethyl sulfoxide) with dissolved NdCl3 has been researched and has given promising results. The study of this electrolytic system has been divided into two stages. Firstly, the characteristics of the electrolyte, the dissolution of NdCl3 in DMSO, the conductivity and the viscosity of NdCl3 solutions in DMSO at various temperatures, and the Nd complexation in the solution were studied and secondly, the electrolysis parameters and their impact on the Nd electrodeposition process were evaluated. Finally, the deposits were submitted to SEM-EDS (Scanning Electron Microscopy-Energy Dispersive X-Ray Spectroscopy) analysis and metallic Nd was confirmed to be electrodeposited by X-ray Photoelectron Spectroscopy (XPS) spectroscopy.
Genome-Wide Investigation of Genes Regulated by ERα in Breast Cancer Cells
Authors: Wang Shuning , Li Xiaoju , Zhang Wangqian , Gao Yuan , Zhang Kuo , Hao Qiang , Li Weina , Wang Zhaowei , Li Meng , Zhang Wei , Zhang Yingqi , Zhang Cun
Published: October 2018

Estrogen receptor alpha (ERα), which has been detected in over 70% of breast cancer cases, is a driving factor for breast cancer growth. For investigating the underlying genes and networks regulated by ERα in breast cancer, RNA-seq was performed between ERα transgenic MDA-MB-231 cells and wild type MDA-MB-231 cells. A total of 267 differentially expressed genes (DEGs) were identified. Then bioinformatics analyses were performed to illustrate the mechanism of ERα. Besides, by comparison of RNA-seq data obtained from MDA-MB-231 cells and microarray dataset obtained from estrogen (E2) stimulated MCF-7 cells, an overlap of 126 DEGs was screened. The expression level of ERα was negatively associated with metastasis and EMT in breast cancer. We further verified that ERα might inhibit metastasis by regulating of VCL and TNFRSF12A, and suppress EMT by the regulating of JUNB and ID3. And the relationship between ERα and these genes were validated by RT-PCR and correlation analysis based on TCGA database. By PPI network analysis, we identified TOP5 hub genes, FOS, SP1, CDKN1A, CALCR and JUNB, which were involved in cell proliferation and invasion. Taken together, the whole-genome insights carried in this work can help fully understanding biological roles of ERα in breast cancer.
Separation and Bioactive Assay of 25R/S-Spirostanol Saponin Diastereomers from Yucca schidigera Roezl (Mojave) Stems
Authors: Qu Lu , Ruan Jingya , Wu Song , Huang Peijian , Yan Jiejing , Yu Haiyang , Zhang Yi , Wang Tao
Published: October 2018

In order to find a simple, generic, efficient separation method for 25R/S-spirostanol saponin diastereomers, the liquid chromatographic retention behaviors of C12 carbonylation and C12 unsubstituted 25R/S-spirostanol saponin diastereomers on different stationary phases (C8, C18, C30 columns) and different mobile phases (MeOH-1% CH3COOH and CH3CN-1% CH3COOH) were investigated. A C30 column was firstly found to offer the highest efficiency for the separation of this kind of diastereomers than C8 and C18 columns. Meanwhile, the analysis results indicated that both CH3CN-1% CH3COOH and MeOH-1% CH3COOH eluate systems were selective for C12 unsubstituted 25R/S-spirostanol saponin diastereomers, while MeOH-1% CH3COOH possessed better selectivity for C12 carbonylation ones. Using the abovementioned analysis method, six pairs of 25R/S-spirostanol saponin diastereomers 1a–6a and 1b–6b from Yucca schidigera Roezl (Mojave) were isolated successfully by using HPLC on C30 column for the first time. Among them, three pairs were new ones, named as (25R)-Yucca spirostanoside E1 (1a), (25S)-Yucca spirostanoside E1 (1b), (25R)-Yucca spirostanoside E2 (2a), (25S)-Yucca spirostanoside E2 (2b), (25R)-Yucca spirostanoside E3 (3a), (25S)-Yucca spirostanoside E3 (3b), respectively. Moreover, 3a, 5a, 6a, 3b–6b showed strong inhibitory activities on the growth of SW620 cell lines with the IC50 values of 12.02–69.17 μM.
A Comprehensive Study on the Biological Activity of Elderberry Extract and Cyanidin 3-O-Glucoside and Their Interactions with Membranes and Human Serum Albumin
Authors: Strugała Paulina , Loi Sabrina , Bażanów Barbara , Kuropka Piotr , Kucharska Alicja Z. , Włoch Aleksandra , Gabrielska Janina
Published: October 2018

In our research we used the extract from dietary supplement of elderberry (EE) and its dominant anthocyanin—cyanidin 3-O-glucoside (Cy 3-gluc). By interacting with a model membrane that reflects the main lipid composition of tumor membranes, the extract components, including Cy 3-gluc, caused an increase in packing order, mainly in the hydrophilic region of the membrane. It can thus be stated that EE caused a rigidifying effect, which is fundamental for understanding its anticancer and antioxidant activity. This study represents the first attempt to unravel the mechanism of interaction of elderberry extract with membranes. The results of the interaction with human serum albumin (HSA) proved that the studied substance quenches the fluorescence of HSA through a static mechanism in which the main interaction forces are Van der Waals and hydrogen bonding. The antioxidant activity of EE and Cy 3-gluc on liposomal membranes, antiradical properties and ability to inhibited the activity of the enzymes cyclooxygenase-1 and cyclooxygenase-2 were also demonstrated. Moreover, the anticancer activity of EE and Cy 3-gluc on human breast adenocarcinoma cell line were investigated. In addition, EE also exhibited the ability to form lipid aggregates in the form of liposomal capsules that can be applied as carriers of active biological substances, and the highest efficacy of EE encapsulation was obtained for multilayered liposome formulations.
Design and Evaluation of a Mixed-Reality Playground for Child-Robot Games
Authors: Lupetti Maria Luce , Piumatti Giovanni , Germak Claudio , Lamberti Fabrizio
Published: October 2018

In this article we present the Phygital Game project, a mixed-reality game platform in which children can play with or against a robot. The project was developed by adopting a human-centered design approach, characterized by the engagement of both children and parents in the design process, and situating the game platform in a real context—an educational center for children. We report the results of both the preliminary studies and the final testing session, which focused on the evaluation of usability factors. By providing a detailed description of the process and the results, this work aims at sharing the findings and the lessons learned about both the implications of adopting a human-centered approach across the whole design process and the specific challenges of developing a mixed-reality playground.
Isolation of Nb2Se9 Molecular Chain from Bulk One-Dimensional Crystal by Liquid Exfoliation
Authors: Chae Sudong , Siddiqa Akhtar J. , Oh Seungbae , Kim Bum Jun , Choi Kyung Hwan , Jang Woo-Sung , Kim Young-Min , Yu Hak Ki , Choi Jae-Young
Published: October 2018

The optimum solvent for Nb2Se9 dispersion, which is a new type of one dimensional (1D) material, is investigated. Among several solvents (16 solvents in total), strong dispersion was observed in benzyl alcohol, isopropyl alcohol, isobutyl alcohol, and diacetone alcohol, which have medium dielectric constants in the range of 10 to 30 and surface tension in the range of 25 to 35 mJ m−2. 1D Nb2Se9 chains, whose size is less than 10 nm, are well dispersed and it is possible to disperse mono-chains of 1 nm or less in a specific dispersion region. The 1D unit chain with dangling bond free surface and high volume to area ratio is expected to be used in applications that utilize the surface of the material. Such dispersion is an important first step towards various potential applications and is an indispensable scientific goal for the practical applications of Nb2Se9.
Cycle Stability and Hydration Behavior of Magnesium Oxide and Its Dependence on the Precursor-Related Particle Morphology
Authors: Gravogl Georg , Knoll Christian , Welch Jan M. , Artner Werner , Freiberger Norbert , Nilica Roland , Eitenberger Elisabeth , Friedbacher Gernot , Harasek Michael , Werner Andreas , Hradil Klaudia , Peterlik Herwig , Weinberger Peter , Müller Danny , Miletich Ronald
Published: October 2018

Thermochemical energy storage is considered as an auspicious method for the recycling of medium-temperature waste heat. The reaction couple Mg(OH)2–MgO is intensely investigated for this purpose, suffering so far from limited cycle stability. To overcome this issue, Mg(OH)2, MgCO3, and MgC2O4·2H2O were compared as precursor materials for MgO production. Depending on the precursor, the particle morphology of the resulting MgO changes, resulting in different hydration behavior and cycle stability. Agglomeration of the material during cyclization was identified as main reason for the decreased reactivity. Immersion of the spent material in liquid H2O decomposes the agglomerates restoring the initial reactivity of the material, thus serving as a regeneration step.
Operation Mechanism of a MoS2/BP Heterojunction FET
Authors: Lim Sung Kwan , Kang Soo Cheol , Yoo Tae Jin , Lee Sang Kyung , Hwang Hyeon Jun , Lee Byoung Hun
Published: October 2018

The electrical characteristics and operation mechanism of a molybdenum disulfide/black phosphorus (MoS2/BP) heterojunction device are investigated herein. Even though this device showed a high on-off ratio of over 1 × 107, with a lower subthreshold swing of ~54 mV/dec and a 1fA level off current, its operating mechanism is closer to a junction field-effect transistor (FET) than a tunneling FET. The off-current of this device is governed by the depletion region in the BP layer, and the band-to-band tunneling current does not contribute to the rapid turn-on and extremely low off-current.
Passive Sampling of Gaseous Elemental Mercury Based on a Composite TiO2NP/AuNP Layer
Authors: Macagnano Antonella , Papa Paolo , Avossa Joshua , Perri Viviana , Marelli Marcello , Sprovieri Francesca , Zampetti Emiliano , De Cesare Fabrizio , Bearzotti Andrea , Pirrone Nicola
Published: October 2018

Passive sampling systems (PASs) are a low cost strategy to quantify Hg levels in air over both different environmental locations and time periods of few hours to weeks/months. For this reason, novel nanostructured materials have been designed and developed. They consist of an adsorbent layer made of titania nanoparticles (TiO2NPs, ≤25 nm diameter) finely decorated with gold nanoparticles. The TiO2NPs functionalization occurred for the photocatalytic properties of titania-anatase when UV-irradiated in an aqueous solution containing HAuCl4. The resulting nanostructured suspension was deposited by drop-casting on a thin quartz slices, dried and then incorporated into a common axial sampler to be investigated as a potential PAS device. The morphological characteristics of the sample were studied by High-Resolution Transmission Electron Microscopy, Atomic Force Microscopy, and Optical Microscopy. UV-Vis spectra showed a blue shift of the membrane when exposed to Hg0 vapors. The adsorbed mercury was thermally desorbed for a few minutes, and then quantified by a mercury vapor analyzer. Such a sampling system reported an efficiency of adsorption that was equal to ≈95%. Temperature and relative humidity only mildly affected the membrane performances. These structures seem to be promising candidates for mercury samplers, due to both the strong affinity of gold with Hg, and the wide adsorbing surface.
Preparation of Self-supporting Bagasse Cellulose Nanofibrils Hydrogels Induced by Zinc Ions
Authors: Lu Peng , Liu Ren , Liu Xin , Wu Min
Published: October 2018

Cellulose hydrogels are often prepared from native cellulose through a direct cellulose dissolution approach that often involves tedious process and solvent recovery problems. A self-supporting cellulose hydrogel was prepared by gelation of the TEMPO-oxidized bagasse cellulose nanofibrils (CNF) triggered by strong crosslinking between carboxylate groups and Zn2+. TEMPO process was used to generate negatively charged carboxylate groups on CNF surface to provide a high binding capability to Zn2+. Three TEMPO-oxidized CNFs of different carboxylate contents were prepared and characterized. TEM and AFM microscopes suggested that the sizes of CNFs were fined down and carboxylated cellulose nanofibrils (TOCNFs) of 5–10 nm wide, 200–500 nm long, and carboxylate contents 0.73–1.29 mmol/g were obtained. The final structures and compressive strength of hydrogels were primarily influenced by interfibril Zn2+-carboxylate interactions, following the order of TOCNFs concentration > content of carboxylate groups > concentration of zinc ions. A CO2 sensitive self-supporting cellulose hydrogel was developed as a colorimetric indicator of food spoilage for intelligent food packaging applications.
Characteristics of Graphene Oxide Films Reduced by Using an Atmospheric Plasma System
Authors: Yang Chii-Rong , Tseng Shih-Feng , Chen Yu-Ting
Published: October 2018

The chemical oxidation method can be used to mass-produce graphene oxides (GOs) from highly oriented pyrolytic graphite. However, numerous oxygen-containing functional groups (hydroxyl, epoxy, carbonyl, etc.) exist in typical GO surfaces, resulting in serious electrical losses. Hence, GO must be processed into reduced graphene oxide (rGO) by the removal of most of the oxygen-containing functional groups. This research concentrates on the reduction efficiency of GO films that are manufactured using atmospheric-pressure and continuous plasma irradiation. Before and after sessions of plasma irradiation with various irradiation times, shelters, and working distances, the surface, physical, and electrical characteristics of homemade GO and rGO films are measured and analyzed. Experimental results showed that the sheet resistance values of rGO films with silicon or quartz shelters were markedly lower than those of GO films because the rGO films were mostly deprived of oxygen-containing functional groups. The lowest sheet resistance value and the largest carbon-to-oxygen ratio of typical rGO films were approximately 90 Ω/sq and 1.522, respectively. The intensity of the C–O bond peak in typical rGO films was significantly lower than that in GO films. Moreover, the intensity of the C–C bond peak in typical rGO films was considerably higher than that in GO films.
Copaifera langsdorffii Novel Putative Long Non-Coding RNAs: Interspecies Conservation Analysis in Adaptive Response to Different Biomes
Authors: Danilevicz Monica F. , Moharana Kanhu C. , Venancio Thiago M. , Franco Luciana O. , Cardoso Sérgio R. S. , Cardoso Mônica , Thiebaut Flávia , Hemerly Adriana S. , Prosdocimi Francisco , Ferreira Paulo C. G.
Published: October 2018

Long non-coding RNAs (lncRNAs) are involved in multiple regulatory pathways and its versatile form of action has disclosed a new layer in gene regulation. LncRNAs have their expression levels modulated during plant development, and in response to stresses with tissue-specific functions. In this study, we analyzed lncRNA from leaf samples collected from the legume Copaifera langsdorffii Desf. (copaíba) present in two divergent ecosystems: Cerrado (CER; Ecological Station of Botanical Garden in Brasília, Brazil) and Atlantic Rain Forest (ARF; Rio de Janeiro, Brazil). We identified 8020 novel lncRNAs, and they were compared to seven Fabaceae genomes and transcriptomes, to which 1747 and 2194 copaíba lncRNAs were mapped, respectively, to at least one species. The secondary structures of the lncRNAs that were conserved and differentially expressed between the populations were predicted using in silico methods. A few selected lncRNA were confirmed by RT-qPCR in the samples from both biomes; Additionally, the analysis of the lncRNA sequences predicted that some might act as microRNA (miRNA) targets or decoys. The emerging studies involving lncRNAs function and conservation have shown their involvement in several types of biotic and abiotic stresses. Thus, the conservation of lncRNAs among Fabaceae species considering their rapid turnover, suggests they are likely to have been under functional conservation pressure. Our results indicate the potential involvement of lncRNAs in the adaptation of C. langsdorffii in two different biomes.
Headache Associated with Coeliac Disease: A Systematic Review and Meta-Analysis
Authors: Zis Panagiotis , Julian Thomas , Hadjivassiliou Marios
Published: October 2018

Objective: The aim of this systematic review was to explore the relationship between coeliac disease (CD) and headache. The objectives were to establish the prevalence of each entity amongst the other, to explore the role of gluten free diet (GFD), and to describe the imaging findings in those affected by headaches associated with CD. Methodology: A systematic computer-based literature search was conducted on the PubMed database. Information regarding study type, population size, the age group included, prevalence of CD amongst those with headache and vice versa, imaging results, the nature of headache, and response to GFD. Results: In total, 40 articles published between 1987 and 2017 qualified for inclusion in this review. The mean pooled prevalence of headache amongst those with CD was 26% (95% CI 19.5–33.9%) in adult populations and 18.3% (95% CI 10.4–30.2%) in paediatric populations. The headaches are most often migraine-like. In children with idiopathic headache, the prevalence of CD is 2.4% (95% CI 1.5–3.7%), whereas data for adult populations is presently unavailable. Brain imaging can be normal, although, cerebral calcifications on CT, white matter abnormalities on MRI and deranged regional cerebral blood flow on SPECT can be present. GFD appears to be an effective management for headache in the context of CD, leading to total resolution of headaches in up to 75% of patients. Conclusions: There is an increased prevalence of CD amongst idiopathic headache and vice versa. Therefore, patients with headache of unknown origin should be screened for CD, as such patients may symptomatically benefit from a GFD.
Do the Effects of Resveratrol on Thermogenic and Oxidative Capacities in IBAT and Skeletal Muscle Depend on Feeding Conditions?
Authors: Milton-Laskibar Iñaki , Aguirre Leixuri , Etxeberria Usune , Milagro Fermin I. , Martínez J. Alfredo , Portillo Maria P.
Published: October 2018

The aim of this study was to compare the effects of mild energy restriction and resveratrol on thermogenic and oxidative capacity in interscapular brown adipose tissue (IBAT) and in skeletal muscle. Rats were fed a high-fat high-sucrose diet for six weeks, and divided into four experimental groups fed a standard diet: a control group, a resveratrol-treated group, an energy-restricted group and an energy-restricted group treated with resveratrol. Weights of IBAT, gastrocnemius muscle and fat depots were measured. Activities of carnitine palmitoyltransferase (CPT) and citrate synthase (CS), protein levels of sirtuin (SIRT1 and 3), uncoupling proteins (UCP1 and 3), glucose transporter (GLUT4), mitochondrial transcription factor (TFAM), nuclear respiratory factor (NRF1), peroxisome proliferator-activated receptor (PPARα) and AMP activated protein kinase (AMPK) and peroxisome proliferator-activated receptor gamma coactivator (PGC1α) activation were measured. No changes in IBAT and gastrocnemius weights were found. Energy-restriction, but not resveratrol, decreased the weights of adipose depots. In IBAT, resveratrol enhanced thermogenesis activating the SIRT1/PGC1α/PPARα axis. Resveratrol also induced fatty acid oxidation and glucose uptake. These effects were similar when resveratrol was combined with energy restriction. In the case of gastrocnemius muscle, the effects were not as clear as in the case of IBAT. In this tissue, resveratrol increased oxidative capacity. The combination of resveratrol and energy restriction seemingly did not improve the effects induced by the polyphenol alone.
Mitigated Impact of Provision of Local Foods Combined with Nutrition Education and Counseling on Young Child Nutritional Status in Cambodia
Authors: Menasria Lylia , Blaney Sonia , Main Barbara , Vong Lenin , Hun Vannary , Raminashvili David , Chhea Chhorvann , Chiasson Lucie , Leblanc Caroline P.
Published: October 2018

Background: In Cambodia, stunting and wasting affect, respectively, 32% and 10% of children 0–59 months while 55% are anemic. Our research aims to assess the efficiency of two local foods combined with nutritional education and counseling (CEN) activities as compared to CEN alone on improving child nutritional status and dietary intake. Methods: A cluster-randomized controlled trial was conducted in Soth Nikum area over a six-month period among children 6–23 months (n = 360) assigned to receive either moringa +CEN, cricket +CEN or CEN alone. Anthropometric measurements were performed and hemoglobin and ferritin levels assessed. Results: Overall, no significant increase in the mean length/height-for-age z-score was observed, although a small increase of the weight-for-length/height was noted in intervention groups. Hemoglobin and ferritin mean values increased in all groups. The degree of satisfaction of energy, proteins, iron, and zinc requirements improved in all groups, but to a greater extent in the intervention groups and more children were healthy. Conclusion: Our research shows no significant impact of the provision of two local foods combined with CEN on the improvement of child nutritional status as compared to CEN alone. However, children consuming them better fulfilled their energy, iron, and zinc requirements and were healthier.