bluuebunny/crossref_metadata_2025 huggingface dataset:
Organic–inorganic hybrid salt and mixed ligand Cr(III) complexes containing the natural flavonoid chrysin: Synthesis, characterization, computational, and biological studies
Authors: Alem Mamaru Bitew , Desalegn Tegene , Damena Tadewos , Bayle Enyew Alemayehu , Koobotse Moses O. , Ngwira Kennedy J. , Ombito Japheth O. , Zachariah Matshediso , Demissie Taye B.
Published: April 2023

Organic–inorganic hybrid salt and mixed ligand Cr(III) complexes (Cr1 and Cr2) containing the natural flavonoid chrysin were synthesized. The metal complexes were characterized using UV-Vis, Fourier-transform infrared, MS, SEM-EDX, XRD, and molar conductance measurements. Based on experimental and DFT/TD-DFT calculations, octahedral geometries for the synthesized complexes were suggested. The powder XRD analysis confirms that the synthesized complexes were polycrystalline, with orthorhombic and monoclinic crystal systems having average crystallite sizes of 21.453 and 19.600 nm, percent crystallinities of 51% and 31.37%, and dislocation densities of 2.324 × 10−3 and 2.603 × 10−3 nm-2 for Cr1 and Cr2, respectively. The complexes were subjected to cytotoxicity, antibacterial, and antioxidant studies. The in vitro biological studies were supported with quantum chemical and molecular docking computational studies. Cr1 showed significant cytotoxicity to the MCF-7 cell line, with an IC50 value of 8.08 μM compared to 30.85 μM for Cr2 and 18.62 μM for cisplatin. Cr2 showed better antibacterial activity than Cr1. The higher EHOMO (−5.959 eV) and dipole moment (10.838 Debye) values of Cr2 obtained from the quantum chemical calculations support the observed in vitro antibacterial activities. The overall results indicated that Cr1 is a promising cytotoxic drug candidate.
Bibliometric and visualized analysis of elite controllers based on CiteSpace: landscapes, hotspots, and frontiers
Authors: Yuan Xingyue , Lai Yu
Published: April 2023

Background

A unique subset of people living with HIV, known as elite controllers, possess spontaneous and consistent control over viral replication and disease progression in the absence of antiviral intervention. In-depth research on elite controllers is conducive to designing better treatment strategies for HIV. However, comprehensive and illuminating bibliometric reports on elite controllers are rare.

Methods

Articles on elite controllers were retrieved from the Web of Science Core Collection. A visualized analysis of this domain was conducted by CiteSpace software. Taking count, betweenness centrality, and burst value as criteria, we interpreted the visualization results and predicted future new directions and emerging trends.

Results

By December 31, 2022, 843 articles related to elite controllers had been published. The largest contributors in terms of country, institution, and author were the United States (485), Univ Calif San Francisco (87), and Walker B.D. (65), respectively. Migueles S.A. (325) and Journal of Virology (770) were the most cocited author and journal, respectively. Additionally, by summarizing the results of our CiteSpace software analysis on references and keywords, we considered that the research hotspots and frontiers on elite controllers mainly focus on three aspects: deciphering the mechanisms of durable control, delineating the implications for the development of treatments for HIV infection, and highlighting the clinical risks faced by elite controllers and coping strategies.

Conclusion

This study performed a bibliometric and visual analysis of elite controllers, identified the main characteristics and emerging trends, and provided insightful references for further development of this rapidly evolving and complex field.
The epithelial sodium channel in inflammation and blood pressure modulation
Authors: Ahmad Taseer , Ertuglu Lale A. , Masenga Sepiso K. , Kleyman Thomas R. , Kirabo Annet
Published: April 2023

A major regulator of blood pressure and volume homeostasis in the kidney is the epithelial sodium channel (ENaC). ENaC is composed of alpha(α)/beta(β)/gamma(γ) or delta(δ)/beta(β)/gamma(γ) subunits. The δ subunit is functional in the guinea pig, but not in routinely used experimental rodent models including rat or mouse, and thus remains the least understood of the four subunits. While the δ subunit is poorly expressed in the human kidney, we recently found that its gene variants are associated with blood pressure and kidney function. The δ subunit is expressed in the human vasculature where it may influence vascular function. Moreover, we recently found that the δ subunit is also expressed human antigen presenting cells (APCs). Our studies indicate that extracellular Na+ enters APCs via ENaC leading to inflammation and salt-induced hypertension. In this review, we highlight recent findings on the role of extra-renal ENaC in inflammation, vascular dysfunction, and blood pressure modulation. Targeting extra-renal ENaC may provide new drug therapies for salt-induced hypertension.
Myocardial deformation in malignant mitral valve prolapse: A shifting paradigm to dynamic mitral valve–ventricular interactions
Authors: Pace Nathalie , Sellal Jean-Marc , Venner Clement , Mandry Damien , Marie Pierre-Yves , Filippetti Laura , Echivard Mathieu , Fraix Antoine , Girerd Nicolas , Lamiral Zohra , De Chillou Christian , Sadoul Nicolas , Selton-Suty Christine , Huttin Olivier
Published: April 2023

Objectives

This study sought to assess the value of myocardial deformation using strain echocardiography in patients with mitral valve prolapse (MVP) and severe ventricular arrhythmia and to evaluate its impact on rhythmic risk stratification.

Background

MVP is a common valvular affection with an overly benign course. Unpredictably, selected patients will present severe ventricular arrhythmia.

Methods

Patients with MVP as the only cause of aborted SCD (MVP-aSCD: ventricular fibrillation and monomorphic and polymorphic ventricular tachycardia) with no other obvious reversible cause were identified. Nonconsecutive patients referred for the echocardiographic evaluation of MVP were enrolled as a control cohort and dichotomized according to the presence or absence of premature ventricular contractions (MVP-PVC or MVP-No PVC, respectively). All patients had a comprehensive strain assessment of mechanical dispersion (MD), postsystolic shortening, and postsystolic index (PSI).

Results

A total of 260 patients were enrolled (20 MVP-aSCD, 54 MVP-PVC, and 186 MVP-No PVC). Deformation pattern discrepancies were observed with a higher PSI value in MVP-aSCD than that in MVP-PVC (4.6 ± 2.0 vs. 2.9 ± 3.7, p = 0.014) and a higher MD value than that in MVP-No PVC (46.0 ± 13.0 vs. 36.4 ± 10.8, p = 0.002). In addition, PSI and MD increased the prediction of severe ventricular arrhythmia on top of classical risk factors in MVP. Net reclassification improvement was 61% (p = 0.008) for PSI and 71% (p = 0.001) for MD.

Conclusions

In MVP, myocardial deformation analysis with strain echocardiography identified specific contraction patterns with postsystolic shortening leading to increased values of PSI and MD, translating the importance of mitral valve–myocardial interactions in the arrhythmogenesis of severe ventricular arrhythmia. Strain echocardiography may provide important implications for rhythmic risk stratification in MVP.
Clinical outcomes of a CT protocol for simultaneous examination of the aorta and coronary artery in patients with aortic aneurysm
Authors: Kim Hoyoung , Kim Jihoon , Choe Yeon Hyeon , Kim Sung Mok
Published: April 2023

Objectives

In patients with aortic aneurysm (AA), coronary artery disease (CAD) increases the risk of perioperative complications and even asymptomatic CAD is associated with adverse clinical outcomes. We aimed to compare coronary-aorta CT (CACT) with thoracoabdominal CT angiography (Aorta CT) for CAD management and clinical outcomes in these patients.

Methods

We enrolled 479 patients undergoing CACT and 693 patients undergoing Aorta CT as an initial CT scan for AA. The primary outcome was a composite of all-cause death or myocardial infarction (MI) at 3 years after CT. The secondary outcomes were subsequent CAD management and invasive coronary angiography (CAG).

Results

After index CT scan, the CACT group had a significantly higher rate of coronary revascularization compared with the Aorta CT group (10.7% vs. 3.8%, p < 0.001) but a lower probability of diagnostic CAG among total invasive CAG (32% vs. 55%, p < 0.001). At 3 months after the CT scan, the prescription rates of statins (65.8% vs. 44.6%, p < 0.001) and antiplatelet agents (57.6% vs. 43.9%, p < 0.001) were higher in the CACT group. During follow-up, the CACT group had a significantly lower incidence of the composite outcome of all-cause death or MI (adjusted HR 1.72, 95% CI 1.07–2.78, p = 0.027) than the Aorta CT group.

Conclusion

Among patients with AA, CACT was associated with a higher rate of subsequent CAD management and a lower risk of all-cause death or MI compared to Aorta CT. When evaluating with AA using CT, simultaneous coronary and aortic evaluation using CACT would be recommended over Aorta CT.
Executive functions and classroom behaviour in second graders
Authors: Heemskerk Christina Hubertina Helena Maria , Roebers Claudia M.
Published: April 2023

Background

Executive functions along with on-task behaviour in the classroom relate to academic success. Examining the shared and non-shared variances in their relationships with academic achievement may lead to a better understanding of the contribution of executive functions to achievement and may uncover a mechanism to explain why they are so important for school success. Specifically, we investigated the extent to which executive functions and classroom behaviour offer different and similar perspectives on children’s self-regulatory behaviour, and make unique contributions to academic achievement.

Method

Data were collected from 129 2nd grade students (M age = 7.93 years, SD = 0.5; 44.2% female). Participants were observed for 25 min during a ‘business-as-usual’ classroom lesson, following a momentary assessment protocol; we developed and used an openly available mobile application. Subsequently, participants completed an executive function task (Hearts and Flowers) in small groups on tablet computers. Teachers rated students’ academic achievement in language and mathematics.

Results

We found unique contributions for on-task behaviour and executive functions to academic achievement in 2nd grade. Moreover, we found that 27–31% of the total effect of inhibition on language achievement was mediated through on-task behaviour.

Conclusion

We have shown that executive functions and on-task behaviour share variance in their relationship to academic achievement, as well as providing a unique perspective on children’s self-regulatory behaviour. Thus, researchers might want consider the inclusion of both executive function tasks and ecologically valid measures such as the current, easy to apply behavioural observation in a naturalistic setting in their future work.
A rare occurrence of non-classic congenital adrenal hyperplasia and type 1 diabetes mellitus in a girl with Prader-Willi Syndrome: Case report and review of the literature
Authors: Aureli Alessia , Bocchini Sarah , Mariani Michela , Crinò Antonino , Cappa Marco , Fintini Danilo
Published: April 2023

Prader–Willi syndrome (PWS) is a rare genetic disorder resulting from lack of expression of the paternally derived chromosome 15q11–13, associated with several complications, including pubertal disorders, short stature, hyperphagia, obesity, glucose metabolism abnormalities, scoliosis, obstructive sleep apnea syndrome (OSAS) and behavioral problems. We report the case of a girl affected by PWS who presented at the age of 5.9 with premature pubarche, accelerated linear growth and advanced bone age (BA). She was subsequently diagnosed with non-classic congenital adrenal hyperplasia (CAH) confirmed by genetic analysis. Considering the clinical, biochemical, and genetic findings, hydrocortisone therapy was started to prevent rapid BA acceleration and severe compromission of final height. During infancy, short stature and low levels of insulin-like growth factor-1 (IGF-1) for age and gender led to suspicion of growth hormone deficiency (GHD), confirmed by stimulation testing (arginine and clonidine). rhGH therapy was administered and continued until final height was reached. During endocrinological follow up she developed impaired glucose tolerance with positive markers of β-cell autoimmunity (anti-glutamic acid decarboxylase antibodies, GAD Ab), which evolved over time into type 1 diabetes mellitus and insulin therapy with a basal-bolus scheme and an appropriate diet were needed.
Visual analysis of alpine meadow research trends and hotspots based on VOS viewer
Authors: Lin Weishan , De Kejia , Wei Xijie , Wang Wei , Zhang Lin , Xiang Xuemei , Li Fei
Published: April 2023

In order to reveal the overall research progress in the field of alpine meadows. In this study, a visual clustering analysis of the literature was conducted using VOS viewer software. The English literature related to alpine meadow was searched in the Web of Science database with publication dates limited to 2020–2021, and 3,607 papers were retrieved from the Web of Science using Excel software. By analyzing the basic profiles of annual publication volume, publication country/region, publication journal, publication institution, publication author, and keywords, the hot spots and development trends of alpine meadow research were derived. The data show that China is the top global country for alpine meadow research in the world, the institution with the most publications in Chinese Acad Sci, and the most publications are by Huakun Zhou from China (81 articles); “enzymes”, “climate change” and “microorganisms” are the current hot spots for alpine meadow research. This study analyzes the publication situation, research hotspots and research trends in the field of alpine meadow research to provide a reference for the academic research on alpine meadows for those related to this field.
Exploring the reasons why mothers do not breastfeed, to inform and enable better support
Authors: Roberts Dean , Jackson Leanne , Davie Philippa , Zhao Catherine , Harrold Joanne A. , Fallon Victoria , Silverio Sergio A.
Published: April 2023

Introduction

Infant and maternal breastfeeding benefits are well documented, globally. Despite efforts to increase global breastfeeding rates, the majority of high-income settings fall short of recommended targets. Breastfeeding rates in the UK are especially poor, and physiological difficulties (e.g., inverted nipples), fail to account for the observed breastfeeding intention-behaviour gap.

Method

The current online study sought to investigate the infant feeding experiences of 624 UK formula feeding mothers, through open text survey responses.

Results

A content analysis identified the following clusters of reasons for formula feeding: Feeding Attitudes, Feeding Problems, Mental Health, and Sharing the Load.

Discussion

Feeding Attitudes explained a large percentage of reasons given for formula feeding. Recommendations are made to improve antenatal breastfeeding education and to develop an intervention with an aim to improve maternal breastfeeding attitudes and subsequent practice. Feeding Problems also explained a large portion of combination feeding and started but stopped infant feeding accounts. The current paper calls for more comprehensive and tailored antenatal breastfeeding education to refine practical breastfeeding skills necessary for successful breastfeeding establishment and maintenance. Mental Health explained relatively small coverage. Suggestions are therefore made to train mental health practitioners on infant feeding with an aim to provide more extensive support, which may serve to disrupt the bidirectional relationship between poor mental health and poor breastfeeding outcomes. Finally, Sharing the Load explained moderate coverage across never breastfed, combination fed, and started but stopped feeding groups. Recommendations are made, in light of these findings, to tighten workplace legislation to protect breastfeeding women.
Cerebrospinal fluid proteomics in recent-onset Narcolepsy type 1 reveals activation of the complement system
Authors: Ayoub Ikram , Dauvilliers Yves , Barateau Lucie , Vermeulen Thaïs , Mouton-Barbosa Emmanuelle , Marcellin Marlène , Gonzalez-de-Peredo Anne , Gross Catharina C. , Saoudi Abdelhadi , Liblau Roland
Published: April 2023

Introduction

Narcolepsy type 1 (NT1) is a rare, chronic and disabling neurological disease causing excessive daytime sleepiness and cataplexy. NT1 is characterized pathologically by an almost complete loss of neurons producing the orexin neuropeptides in the lateral hypothalamus. Genetic and environmental factors strongly suggest the involvement of the immune system in the loss of orexin neurons. The cerebrospinal fluid (CSF), secreted locally and surrounding the central nervous system (CNS), represents an accessible window into CNS pathological processes.

Methods

To gain insight into the biological and molecular changes in NT1 patients, we performed a comparative proteomics analysis of the CSF from 21 recent-onset NT1 patients and from two control groups: group 1 with somatoform disorders, and group 2 patients with hypersomnia other than NT1, to control for any potential effect of sleep disturbances on CSF composition. To achieve an optimal proteomic coverage analysis, the twelve most abundant CSF proteins were depleted, and samples were analyzed by nano-flow liquid chromatography tandem mass spectrometry (nano-LC-MS/MS) using the latest generation of hybrid Orbitrap mass spectrometer.

Results and discussion

Our study allowed the identification and quantification of up to 1943 proteins, providing a remarkably deep analysis of the CSF proteome. Interestingly, gene set enrichment analysis indicated that the complement and coagulation systems were enriched and significantly activated in NT1 patients in both cohorts analyzed. Notably, the lectin and alternative complement pathway as well as the downstream lytic membrane attack complex were congruently increased in NT1. Our data suggest that the complement dysregulation in NT1 patients can contribute to immunopathology either by directly promoting tissue damage or as part of local inflammatory responses. We therefore reveal an altered composition of the CSF proteome in NT1 patients, which points to an ongoing inflammatory process contributed, at least in part, by the complement system.
Cytoplasmic DNAs: Sources, sensing, and roles in the development of lung inflammatory diseases and cancer
Authors: He Xintong , Sun Ye , Lu Jianzhang , Naz Faiza , Ma Shenglin , Liu Jian
Published: April 2023

Cytoplasmic DNA is emerging as a pivotal contributor to the pathogenesis of inflammatory diseases and cancer, such as COVID-19 and lung carcinoma. However, the complexity of various cytoplasmic DNA-related pathways and their crosstalk remains challenging to distinguish their specific roles in many distinct inflammatory diseases, especially for the underlying mechanisms. Here, we reviewed the latest findings on cytoplasmic DNA and its signaling pathways in inflammatory lung conditions and lung cancer progression. We found that sustained activation of cytoplasmic DNA sensing pathways contributes to the development of common lung diseases, which may result from external factors or mutations of key genes in the organism. We further discussed the interplays between cytoplasmic DNA and anti-inflammatory or anti-tumor effects for potential immunotherapy. In sum, this review aids in understanding the roles of cytoplasmic DNAs and exploring more therapeutic strategies.
The influence of high glucose conditions on macrophages and its effect on the autophagy pathway
Authors: Sousa Emanuella S. A. , Queiroz Luiz A. D. , Guimarães João P. T. , Pantoja Kamilla C. , Barros Rafael S. , Epiphanio Sabrina , Martins Joilson O.
Published: April 2023

Introduction

Macrophages are central cells in mediating the inflammatory response.

Objective and Methods

We evaluated the effect of high glucose conditions on the inflammatory profile and the autophagy pathway in Bone-Marrow Derived Macrophages (BMDM) from diabetic (D-BMDM) (alloxan: 60mg/kg, i.v.) and non-diabetic (ND-BMDM) C57BL/6 mice. BMDM were cultured in medium with normal glucose (5.5 mM), or high glucose (25 mM) concentration and were primed with Nigericin (20µM) stimulated with LPS (100 ng/mL) at times of 30 minutes; 2; 4; 6 and 24 hours, with the measurement of IL-6, IL-1β and TNF-α cytokines.

Results

We have further identified changes in the secretion of pro-inflammatory cytokines IL-6, IL-1β and TNF-α, where BMDM showed increased secretion of these cytokines after LPS + Nigericin stimulation. In addition, changes were observed in the autophagy pathway, where the increase in the autophagic protein LC3b and Beclin-1 occurred by macrophages of non-diabetic animals in hyperglycemic medium, without LPS stimulation. D-BMDM showed a reduction on the expression of LC3b and Beclin-1, suggesting an impaired autophagic process in these cells.

Conclusion

The results suggest that hyperglycemia alters the inflammatory pathways in macrophages stimulated by LPS, playing an important role in the inflammatory response of diabetic individuals.
Modifications outside CDR1, 2 and 3 of the TCR variable β domain increase TCR expression and antigen-specific function
Authors: Degirmencay Abdullah , Thomas Sharyn , Mohammed Fiyaz , Willcox Benjamin E. , Stauss Hans J.
Published: April 2023

T cell receptor (TCR) gene modified T cells are a promising form of adoptive cellular therapy against human malignancies and viral infections. Since the first human clinical trial was carried out in 2006, several strategies have been developed to improve the efficacy and safety of TCR engineered T cells by enhancing the surface expression of the introduced therapeutic TCRs whilst reducing the mis-pairing with endogenous TCR chains. In this study, we explored how modifications of framework residues in the TCR variable domains affect TCR expression and function. We used bioinformatic and protein structural analyses to identify candidate amino acid residues in the framework of the variable β domain predicted to drive high TCR surface expression. Changes of these residues in poorly expressed TCRs resulted in improved surface expression and boosted target cell specific killing by engineered T cells expressing the modified TCRs. Overall, these results indicate that small changes in the framework of the TCR variable domains can result in improved expression and functionality, while at the same time reducing the risk of toxicity associated with TCR mis-pairing.
Change in perioperative neutrophil-lymphocyte ratio as a potential predictive biomarker for chronic postsurgical pain and quality of life: an ambispective observational cohort study
Authors: Shu Bin , Xu Fang , Zheng Xuemei , Zhang Yamei , Liu Qi , Li Shiqi , Chen Jie , Chen Yuanjing , Huang He , Duan Guangyou
Published: April 2023

Introduction

Accurate and accessible predictors of chronic postsurgical pain (CPSP) to identify high-risk postsurgical patients are prerequisite for preventive and interventional strategies. We investigated the incidence and risk factors of CPSP after abdominal surgery, with a focus on plasma immunological markers.

Materials and methods

This was a retrospective analysis of patients who underwent abdominal surgery under general anesthesia at a tertiary center between January 2021 and January 2022. The preoperative demographics, laboratory test data, and surgical factors of the participants were collected from the electronic medical record system. Postoperative pain intensity and living conditions at 1 year after discharge from the hospital were assessed via a phone survey. Univariate and multivariate analyses were used to explore independent risk factors associated with CPSP.

Results

A total of 968 patients were included, and 13.53% (n = 131 of 968) of patients reported CPSP 1 year after surgery. Patients with older age, open surgery, higher American Association of Anesthesiologists classification, patient-controlled intravenous analgesia application, longer surgery duration, higher postoperative absolute neutrophil count, and neutrophil-lymphocyte ratio (NLR), lower postoperative absolute lymphocyte count, and higher white blood cell count, were more likely to suffer from CPSP. A changed ratio of NLR (postoperative to preoperative) ≥ 5 significantly correlated with CPSP, moderate to severe pain, maximum numeric rating score since discharge from the hospital, and affected quality of life.

Discussion

The changed ratio of NLR could be used for the early identification of patients at risk for CPSP and affect the quality of life to alert the clinician to undertake further assessment.
Coupling large-spatial scale larval dispersal modelling with barcoding to refine the amphi-Atlantic connectivity hypothesis in deep-sea seep mussels
Authors: Portanier Elodie , Nicolle Amandine , Rath Willi , Monnet Lorraine , Le Goff Gregoire , Le Port Anne-Sophie , Daguin-Thiébaut Claire , Morrison Cheryl L. , Cunha Marina R. , Betters Melissa , Young Craig M. , Van Dover Cindy L. , Biastoch Arne , Thiébaut Eric , Jollivet Didier
Published: April 2023

In highly fragmented and relatively stable cold-seep ecosystems, species are expected to exhibit high migration rates and long-distance dispersal of long-lived pelagic larvae to maintain genetic integrity over their range. Accordingly, several species inhabiting cold seeps are widely distributed across the whole Atlantic Ocean, with low genetic divergence between metapopulations on both sides of the Atlantic Equatorial Belt (AEB, i.e. Barbados and African/European margins). Two hypotheses may explain such patterns: (i) the occurrence of present-day gene flow or (ii) incomplete lineage sorting due to large population sizes and low mutation rates. Here, we evaluated the first hypothesis using the cold seep mussels Gigantidas childressi, G. mauritanicus, Bathymodiolus heckerae and B. boomerang. We combined COI barcoding of 763 individuals with VIKING20X larval dispersal modelling at a large spatial scale not previously investigated. Population genetics supported the parallel evolution of Gigantidas and Bathymodiolus genera in the Atlantic Ocean and the occurrence of a 1-3 Million-year-old vicariance effect that isolated populations across the Caribbean Sea. Both population genetics and larval dispersal modelling suggested that contemporary gene flow and larval exchanges are possible across the AEB and the Caribbean Sea, although probably rare. When occurring, larval flow was eastward (AEB - only for B. boomerang) or northward (Caribbean Sea - only for G. mauritanicus). Caution is nevertheless required since we focused on only one mitochondrial gene, which may underestimate gene flow if a genetic barrier exists. Non-negligible genetic differentiation occurred between Barbados and African populations, so we could not discount the incomplete lineage sorting hypothesis. Larval dispersal modelling simulations supported the genetic findings along the American coast with high amounts of larval flow between the Gulf of Mexico (GoM) and the US Atlantic Margin, although the Blake Ridge population of B. heckerae appeared genetically differentiated. Overall, our results suggest that additional studies using nuclear genetic markers and population genomics approaches are needed to clarify the evolutionary history of the Atlantic bathymodioline mussels and to distinguish between ongoing and past processes.
Thiomicrorhabdus marina sp.nov., an obligate chemolithoautotroph isolated from tidal zone sediment, and genome insight into the genus Thiomicrorhabdus
Authors: Tan Xin-Yun , Liu Xin-Jiang , Li Zhao , Yu Fan , Yang Hui , Du Zong-Jun , Ye Meng-Qi
Published: April 2023

The contribution of microbes to the marine sulfur cycle has received considerable attention in recent years. In this study, a new Gram-stain-negative, aerobic sulfur-oxidizing bacterium, designated strain 6S2-11T, was isolated from tidal zone sediment of the coast of Weihai, China. Strain 6S2-11T was an obligate chemolithoautotroph utilizing thiosulfate as the energy source. Physiological and biochemical experiments, phylogenetic analysis, and comparative genomic analysis were done with strain 6S2-11T. According to genomic analysis, strain 6S2-11T owned a complete thiosulfate oxidation pathway and an untypical nitrogen metabolism pathway. Its relatively small genome also has multiple environmental adaptation mechanisms. The DNA G+C content of strain 6S2-11T was 44.1%. Strain 6S2-11T was observed to grow at 20-37°C (optimum, 35°C), pH 6.0-9.5 (optimum, pH 7.5), and 0.5-5% (w/v) NaCl (optimum, 2.5%). The major cellular fatty acids (>10%) of strain 6S2-11T were Summed Feature 8 (C18:1ω7c/C18:1ω6c), C16:0 and Summed Feature 3 (C16:1ω7c/C16:1ω6c). The comparison of 16S rRNA gene sequences indicated that strain 6S2-11T was most closely to Thiomicrorhabdus xiamenensis G2T (96.8%). Based on the results of phylogenetic analysis, the strain 6S2-11T is a novel specie of the genus Thiomicrorhabdus, for which name Thiomicrorhabdus marina sp.nov. is proposed with the type strain 6S2-11T (=MCCC 1H00523T=KCTC 82994T).
Ascorbate−glutathione cycle involving in response of Bangia fuscopurpurea (Bangiales, Rhodophyta) to hyposalinity
Authors: Niu Citong , Wang Wenjun , Yao Haiqin , Liang Zhourui , Zhang Pengyan , Lu Xiaoping
Published: April 2023

Bangia fuscopurpurea is a widespread intertidal seaweed that is commercially cultured in China. This seaweed is frequently exposed to hyposalinity stress, but little is known about the adaptation mechanisms. Ascorbate−glutathione (AsA−GSH) cycle plays important roles in many organisms under a variety of abiotic stress, including hyposaline stress. In this study, we investigated the response of key metabolites and enzymes involved in the AsA−GSH cycle of B. fuscopurpurea under hyposalinity, with the addition of exogenous GSH and Lbuthionine-sulfoximine (BSO). The quantification of BfAPX gene expression was assessed across varied treatment regimens. And the putative interaction proteins of BfAPX were screened by yeast two hybrid system. It was found that under hyposalinity (15 and/or 0 psu), the content of reduced glutathione (GSH), total glutathione (GSH+ oxidized glutathione, GSSG) and cysteine, the ratio of GSH/GSSG and ascorbic acid (AsA)/ dehydroascorbic acid (DHA), and the activity of ascorbic acid peroxidase (APX) and monodehydroascorbate reductase (MDHAR) was significantly up-regulated. The hyposality-promoted GSH/GSSG was weakened while the glutathione reductase (GR) activity was promoted by adding exogenous GSH and BSO. The hyposality-promoted AsA/DHA ratio was strengthened by exogenous GSH but weakened by BSO. The dehydroascorbate reductase (DHAR) activity had no significant changes either with or without exogenous GSH under all salinities, while DHAR activity together with DHA content was enhanced by BSO. The expression of APX gene markedly increased under hyposalinity+BSO treatment. Putative interacting proteins of APX, including glutamate dehydrogenase 1a and fructose diphosphate aldolase, were identified through screening. The results indicated that the AsA−GSH cycle was involved in response of B. fuscopurpurea to hyposalinity by means of increasing GSH/GSSG ratio (through promoting GSH biosynthesis pathway and GSH regeneration from GSSG by GR catalyzation) and AsA/DHA ratio (promoting AsA regeneration through MDHAR). These findings would contribute to improve the aquaculture of this promising economic species and unveil how intertidal seaweeds address the global climate challenges.
Plantar pressures values related with appearance of mechanical hyperkeratosis before and after surgery of mild hallux valgus
Authors: Martínez-Nova Alfonso , Gascó-López de Lacalle Jaime , Morán-Cortés Juan Francisco , Pedrera-Zamorano Juan Diego , Sánchez-Rodríguez Raquel
Published: April 2023

Background

Hyperkeratoses are thickenings of the stratum corneum, provoked by deviation of the ray and excessive plantar pressures. They are very common under the first metatarsal head (MTH) and on the big toe when there exists hallux valgus. The objective of this study was to assess plantar pressures pre- and post-surgery to try to define the threshold values that could determine the appearance of keratopathies.

Materials and methods

Seventy-nine patients (100 feet) who had undergone percutaneous distal soft-tissue release and the Akin procedure were evaluated prospectively. The BioFoot/IBV® in-shoe system was used for objective baropodometric functional evaluations of the heel, midfoot, first through fifth MTHs, hallux, and lesser toes. The presence or absence of a hyperkeratosis (HK) or plantar callus under the first MTH or hallux was recorded. The average follow-up time at which the measurements were repeated was 28.1 months.

Results

Pre-surgery, 62 feet presented a painful HK on the big toe, while post-surgery, only 9 of the feet presented the same lesion. Patients who presented a prior HK at the first metatarsophalangeal (MTP) joint had a mean pressure of 417.2 ± 254.5 kPa as against a value of 359.6 ± 185.1 kPa for the rest. Post-surgery, these values dropped to 409.8 and 346.3 kPa, respectively.

Conclusion

Patients with HK presented an 11% greater mean pressure than those without. The values obtained with the BioFoot/IBV® system in the present study can therefore be considered predictive of the appearance of HK under the first MTH and on the side of the big toe.
Role of autotaxin in systemic lupus erythematosus
Authors: Tsuchida Yumi , Shoda Hirofumi , Sawada Tetsuji , Fujio Keishi
Published: April 2023

Systemic lupus erythematosus (SLE) is a prototypic systemic autoimmune disease characterized by the production of various autoantibodies and deposition of immune complexes. SLE is a heterogenous disease, and the pattern of organ involvement and response to treatment differs significantly among patients. Novel biological markers are necessary to assess the extent of organ involvement and predict treatment response in SLE. Lysophosphatidic acid is a lysophospholipid involved in various biological processes, and autotaxin (ATX), which catalyzes the production of lysophosphatidic acid in the extracellular space, has gained attention in various diseases as a potential biomarker. The concentration of ATX is increased in the serum and urine of patients with SLE and lupus nephritis. Recent evidence suggests that ATX produced by plasmacytoid dendritic cells may play an important role in the immune system and pathogenesis of SLE. Furthermore, the production of ATX is associated with type I interferons, a key cytokine in SLE pathogenesis, and ATX may be a potential biomarker and key molecule in SLE.
Current Understanding of piRNA in Cardiovascular Diseases
Authors: Cheang Iokfai , Zhu Qingqing , Liao Shengen , Li Xinli
Published: April 2023

The relationship regarding non-coding genomes and cardiovascular disease (CVD) has been explored in the past decade. As one of the leading causes of death, there remains a lack of sensitive and specific genomic biomarkers in the diagnosis and prognosis of CVD. Piwi-interacting RNA (piRNA) is a group of small non-coding RNA (ncRNA) which associated with Piwi proteins. There is an emerging strong body of evidence in support of a role for ncRNAs, including piRNAs, in pathogenesis and prognosis of CVD. This article reviews the current evidence for piRNA-regulated mechanisms in CVD, which could lead to the development of new therapeutic strategies for prevention and treatment.
Digital twin inception in the Era of industrial metaverse
Authors: Mourtzis Dimitris
Published: April 2023

Digital Twins, as a technological pillar of Industry 4.0, correspond to the virtual representation and bi-fold a real-time communication of a digital counterpart of a process or a physical object. As the industrial and manufacturing landscape is shifting towards Industry 5.0, huge investments focusing on enhancing interactions between Operators and Cyber-Physical Systems (CPS) occur. Yet, Metaverse strengthens these interactions as it enables human immersion into a virtual world. Furthermore, it examines the very promising relationships between the CPS, through the digital twins of these CPS. Therefore, this short review presents the concept of the Digital Twin inception in Industrial Metaverse. Additionally, a service-oriented digital twin architecture with Metaverse-enabled platforms for added value creation and interactions with CPS towards achieving Industry 5.0 challenges and beyond is proposed.
Flexible highly conductive films based on expanded graphite /polymer nanocomposites
Authors: Nista Silvia V. G. , Alaferdov Andrei V. , Isayama Yuri H. , Mei Lucia H. I. , Moshkalev Stanislav A.
Published: April 2023

Highly electrically and thermally conducting films of expanded graphite/polymer nanocomposites were fabricated using an approach based on solution mixing methods. The use of Hydroxyethylcellulose and benzylic alcohol based solutions provides efficient dispersion and better exfoliation of multilayer graphene (nanographite) flakes that are further aligned in extended 2D layers forming continuous conductive pathways during lamination (hot calendering) process. Very high electrical conductivity (190 S/cm) was obtained for fabricated layered films. In contrast, for films produced by a conventional mixing and deposition method with acrylic copolymer and the same nanographitic material, with flakes randomly distributed within the composite, much lower conductivities (2.4 S/cm) were obtained.
Motivational decline and proactive response under thermal environmental stress are related to emotion- and problem-focused coping, respectively: Questionnaire construction and fMRI study
Authors: Kawata Kelssy Hitomi dos Santos , Hirano Kanan , Hamamoto Yumi , Oi Hajime , Kanno Akitake , Kawashima Ryuta , Sugiura Motoaki
Published: April 2023

Despite the diversity of human behavioral and psychological responses to environmental thermal stress, the major dimensions of these responses have not been formulated. Accordingly, the relevance of these responses to a framework of coping with stress (i.e., emotion- and problem-focused) and the neural correlates are unexplored. In this study, we first developed a multidimensional inventory for such responses using social surveys and a factor analysis, and then examined the neural correlates of each dimension using a functional magnetic resonance imaging; we manipulated the ambient temperature between uncomfortably hot and cold, and the correlations between the inventory factor scores and discomfort-related neural responses were examined. We identified three factors to construct the inventory: motivational decline, proactive response, and an active behavior, which appeared to reflect inefficient emotion-focused coping, efficient problem-focused coping, and positive appreciation of extreme environmental temperatures, respectively, under environmental thermal stress. Motivational decline score was positively associated with common neural response to thermal stress in the frontal and temporoparietal regions, implicated in emotion regulation, while proactive response score negatively with the neural responses related to subjective discomfort in the medial and lateral parietal cortices, implicated in problem-solving. We thus demonstrated that two of three major dimensions of individual variation in response to and coping with environmental thermal stress conform to an influential two-dimensional framework of stress coping. The current three-dimensional model may expand the frontiers of meteorological human science in both basic and application domains.
Spike timing-dependent plasticity under imbalanced excitation and inhibition reduces the complexity of neural activity
Authors: Park Jihoon , Kawai Yuji , Asada Minoru
Published: April 2023

Excitatory and inhibitory neurons are fundamental components of the brain, and healthy neural circuits are well balanced between excitation and inhibition (E/I balance). However, it is not clear how an E/I imbalance affects the self-organization of the network structure and function in general. In this study, we examined how locally altered E/I balance affects neural dynamics such as the connectivity by activity-dependent formation, the complexity (multiscale entropy) of neural activity, and information transmission. In our simulation, a spiking neural network model was used with the spike-timing dependent plasticity rule to explore the above neural dynamics. We controlled the number of inhibitory neurons and the inhibitory synaptic weights in a single neuron group out of multiple neuron groups. The results showed that a locally increased E/I ratio strengthens excitatory connections, reduces the complexity of neural activity, and decreases information transmission between neuron groups in response to an external input. Finally, we argued the relationship between our results and excessive connections and low complexity of brain activity in the neuropsychiatric brain disorders.
The role of wrist-worn technology in the management of Parkinson’s disease in daily life: A narrative review
Authors: Li Peng , van Wezel Richard , He Fei , Zhao Yifan , Wang Ying
Published: April 2023

Parkinson’s disease (PD) is a neurodegenerative disorder that affects millions of people worldwide. Its slow and heterogeneous progression over time makes timely diagnosis challenging. Wrist-worn digital devices, particularly smartwatches, are currently the most popular tools in the PD research field due to their convenience for long-term daily life monitoring. While wrist-worn sensing devices have garnered significant interest, their value for daily practice is still unclear. In this narrative review, we survey demographic, clinical and technological information from 39 articles across four public databases. Wrist-worn technology mainly monitors motor symptoms and sleep disorders of patients in daily life. We find that accelerometers are the most commonly used sensors to measure the movement of people living with PD. There are few studies on monitoring the disease progression compared to symptom classification. We conclude that wrist-worn sensing technology might be useful to assist in the management of PD through an automatic assessment based on patient-provided daily living information.
Serum short chain fatty acids mediate hippocampal BDNF and correlate with decreasing neuroinflammation following high pectin fiber diet in mice
Authors: Church Jamie S. , Bannish Jane A. M. , Adrian Leighelle A. , Rojas Martinez Kimberly , Henshaw Asari , Schwartzer Jared J.
Published: April 2023

Introduction

Dietary components, such as prebiotic fiber, are known to impact brain chemistry via the gut-brain axis. In particular, short chain fatty acids (SCFAs) generated from excessive soluble fiber consumption are thought to impact neuroimmune signaling and brain function through increased production of neurotropic factors. Given reports that high dietary fiber intake is associated with increased mental health and improved quality of life scores, we set out to identify whether changes in SCFA levels as a result of a high soluble fiber diet mediate hippocampal neuroinflammation and brain derived neurotrophic factor (BDNF) in mice.

Methods

Adult male and female C57BL/6 mice were fed a 1-month high pectin fiber or cellulose-based control diet. Following 1 month of excessive pectin consumption, serum SCFAs were measured using gas chromatography–mass spectrometry (GC-MS) and hippocampal cytokines and BDNF were assessed via multiplex magnetic bead immunoassay.

Results

Pectin-based fiber diet increased circulating acetic acid in both sexes, with no effect on propionic or butyric acid. In the hippocampus, a high fiber diet decreased TNFa, IL-1ß, IL-6, and IFNγ and increased BDNF levels. Furthermore, increased SCFA levels were inversely correlated with neuroinflammation in the hippocampus, with acetic acid revealed as a strong mediator of increased BDNF production.

Conclusion

Collectively, these findings highlight the beneficial effects of fiber-induced molecular changes in a brain region known to influence mood- and cognition-related behaviors. Dietary composition should be considered when developing mental health management plans for men and women with an emphasis on increasing soluble fiber intake.
Association between methionine sulfoxide and risk of moyamoya disease
Authors: Li Junsheng , Ge Peicong , He Qiheng , Liu Chenglong , Zeng Chaofan , Tao Chuming , Zhai Yuanren , Wang Jia , Zhang Qian , Wang Rong , Zhang Yan , Zhang Dong , Zhao Jizong
Published: April 2023

Objective

Methionine sulfoxide (MetO) has been identified as a risk factor for vascular diseases and was considered as an important indicator of oxidative stress. However, the effects of MetO and its association with moyamoya disease (MMD) remained unclear. Therefore, we performed this study to evaluate the association between serum MetO levels and the risk of MMD and its subtypes.

Methods

We eventually included consecutive 353 MMD patients and 88 healthy controls (HCs) with complete data from September 2020 to December 2021 in our analyzes. Serum levels of MetO were quantified using liquid chromatography-mass spectrometry (LC–MS) analysis. We evaluated the role of MetO in MMD using logistic regression models and confirmed by receiver-operating characteristic (ROC) curves and area under curve (AUC) values.

Results

We found that the levels of MetO were significantly higher in MMD and its subtypes than in HCs (p < 0.001 for all). After adjusting for traditional risk factors, serum MetO levels were significantly associated with the risk of MMD and its subtypes (p < 0.001 for all). We further divided the MetO levels into low and high groups, and the high MetO level was significantly associated with the risk of MMD and its subtypes (p < 0.05 for all). When MetO levels were assessed as quartiles, we found that the third (Q3) and fourth (Q4) MetO quartiles had a significantly increased risk of MMD compared with the lowest quartile (Q3, OR: 2.323, 95%CI: 1.088–4.959, p = 0.029; Q4, OR: 5.559, 95%CI: 2.088–14.805, p = 0.001).

Conclusion

In this study, we found that a high level of serum MetO was associated with an increased risk of MMD and its subtypes. Our study raised a novel perspective on the pathogenesis of MMD and suggested potential therapeutic targets.