bluuebunny/crossref_metadata_2025 huggingface dataset:
Specifics of Political and Legal Documents on Cooperation between the EAEU and third Countries (part 2)
Authors: Berezina E. K.
Published: March 2022

This study examines the transregional interaction of the EAEU with third countries within the framework of the concept of “soft power” on the basis of concluded cooperation agreements bearing international legal obligations.

Aim. The study aims to identify the features of the bilateral interaction of the EAEU with Vietnam, Singapore, China, Serbia and Iran within the framework of the concept of “soft power” based on a comparative analysis of political and legal documents on cooperation.

Tasks. The author defines the conceptual foundations of the economic dimension of “soft power”; considers political and legal documents on cooperation concluded between the EAEU and Vietnam, Singapore, China, Serbia, Iran, and identifies the components of “soft power” on the basis of the concluded documents.

Methods. The study uses methods of content analysis and comparative analysis of political and legal documents on cooperation based on criteria such as legal form, institutional mechanism, areas of cooperation to identify the features of bilateral cooperation between the EAEU and Vietnam, Singapore, China, Serbia, Iran and the components of “soft power”.

Results. The study shows that the “soft power” components of political and legal documents on cooperation are more presented in the relationship between the EAEU and Vietnam and Singapore. It is the provisions of these agreements that most often mention scientific and technical cooperation and the implementation of joint research projects, scientific and educational exchange, and education issues. Nevertheless, there are risks in the field of integration interaction between the EAEU and the aforementioned countries, due to both the geographical factor and their specifics in building integration relations with third countries.

Conclusions. Transregional interaction between the EAEU and the above-mentioned countries is aimed at the exchange of information, liberalization and transparency in trade, support for the mechanisms of the World Trade Organization (WTO). An analysis of political and legal documents on cooperation between the EAEU using the example of Vietnam and Singapore demonstrates the opportunities for the EAEU not only to integrate into the global economy and strengthen competitiveness, but also to increase the attractiveness of the EAEU states in terms of promoting the “soft power” strategy.
Ideology of New Era
Authors: Glazyev S. Yu.
Published: March 2022

.
A note on ABC spectral radii of unicyclic graphs
Authors: Yuan Yan
Published: March 2022

The ABC spectral radius of a graph G is the largest eigenvalue of the
ABC matrix modified from the adjacency matrix of G so that the
(u,v)-entry is
√(d+d-2)/√dd
for an edge uv, where d is the degree of vertex w in G.
We show that the graph formed from a cycle of length n-p by attaching p
pendent edges to a vertex uniquely maximizes the ABC spectral radius
over all n-vertex unicyclic graphs with p pendant edges, and over all
n-vertex unicyclic graphs with girth n-p, respectively.
Late onset retinoblastoma in a 12-year-old girl: a case report
Authors: Lazari Catherine , Papageorgiou Eleni , Tsironi Evangelia , Androudi Sofia
Published: March 2022

We report the case of a 12-year-old girl, who presented with floaters
and a white-to-cream peripheral retinal mass in her left eye. No
intrinsic calcifications in B-scan ultrasonography and CT scan of the
orbits were present and MRI showed a homogenously enhancing peripheral
retinal mass with restricted diffusion. Due to close contact with
puppies, the patient was initially treated for ocular toxocariasis.
However, one month later she developed anterior chamber seeding and
anterior chamber tap showed group E retinoblastoma (RB). This case
underlines that RB may occur in older ages with atypical manifestations,
such as uveitis and absence of calcifications.
Practices of antimicrobial usage and associated resistance emergence in smallholder beef cattle production systems in Northern Nigeria: Drivers and One Health challenge
Authors: Alhaji Nma , Hassan abubakar , Adamu Andrew , Odetokun Ismail , Lawan Mohammed , Fasina F. O.
Published: March 2022

Increased animal intensification has led to increased antimicrobial
usage (AMU) with consequent antimicrobial resistance (AMR) development.
This study assess farmers’ practices regarding AMU in beef cattle farms,
explore pathways for AMR dissemination, establish risk status of AMU,
and determine residues presence. We hypothesized socio-economic factors
not to drive antimicrobial misuse and resistance emergence in beef
production systems. Cross-sectional study was conducted on randomly
selected beef farms in Northern Nigeria between 2018 and 2019. Traffic
Light model was used to assess farms’ AMU risk status. Disc Diffusion
Test was performed to detect residues in cattle urine. Descriptive and
multivariable logistic regressions analyses were performed at 95%
confidence level. Of 660 beef cattle farmers selected, 92.1% (n=608)
participated in the study. About 78.9% intensive and 76.6%
semi-intensive farmers do not followed instructions on antimicrobial
dosage. Also, 72.4% of intensive and 83.9.6% semi-intensive farmers do
not observed withdrawal period after AMU on animals. Majorities of
intensive (71.5%) and semi-intensive (53.2%) farmers used
antimicrobials as growth promoters on beef production. Significantly
used antimicrobials on beef cattle were: tetracyclines, sulfonamides,
and penicillin. Pathways for AMR spread from beef animals were:
consumption of contaminated meat (p=0.007); contacts with contaminated
animals and fomites (p<0.001); and contaminated manure and
aerosols in environment (p=0.003) Factors that significantly drive
antimicrobials misuse and resistance emergence were: inappropriate AMU
(OR=2.72; 95% CI:1.93-3.83), non-enforcement of laws (OR=2.98; 95%
CI:2.11-4.21), low education and expertise (OR=1.52; 95% CI:1.09-2.12),
and management system (OR=10.24; 95% CI:6.75-15.54). Traffic Light
model has shown 63.6% intensive and 57.63% semi-intensive farms to be
in Class 3 (Red risk) status. Antimicrobial residues were in 48.4%
intensively managed and 34.4% semi-intensive farms. The study revealed
low practices towards AMU in beef cattle productions. Adequate
sanitation and biosecurity, and application of ‘One Health’ will
mitigate AMR at animal-human-environment interface.
AabZIP1 confers drought tolerance and promotes artemisinin biosynthesis in Artemisia annua
Authors: Shu guoping , Tang yueli , Yuan mingyuan , Jiang chunxue , Liu Wanhong , Zhang Fangyuan , Yang Chunxian , Lan Xiaozhong , Chen Min , Xiang lien , Liao Zhihua
Published: March 2022

Water deficiency is one of the most major factors that limit plant
growth and agricultural productivity. Previous studies showed the
biomass and artemisinin content reduced significantly in A. annua plant
under water scarcity. However, the molecular mechanism by which A. annua
responds to drought stress is not well understood. In this study,
AabZIP1 was identified to be involved in the responsive process to
drought stress in A. annua. AabZIP1 could activate the expression of two
wax biosynthesis genes, AaCER1 and AaCYP86A1, by directly binding to
their promoters. Overexpression of AabZIP1 significantly promoted the
expression of AaCER1 and AaCYP86A1 and led to enhanced cuticular waxes
biosynthesis, thus significantly elevating the tolerance to water
deficiency in A annua. Additionally, AabZIP1 positively regulates the
expression of AaMYC2 by binding to its promoter, a MYC family
transcription factor which positively regulates the expression of
artemisinin biosynthesis genes, like ALDH1, DBR2 and CYP71AV1.
Overexpression of AabZIP1 caused the upregulation of the expression of
AaMYC2 and artemisinin biosynthesis genes, leading to an increase of
artemisinin content. Taken together, our results indicate that AabZIP1
plays a positive role in the regulation of drought tolerance and
artemisinin biosynthesis in A annua.
Occurrence and Morphology of Ventricular Arrhythmias in Apparently Normal Hearts in Relation to Late Gadolinium Enhancement on Cardiovascular Magnetic Resonance
Authors: Qian wen , Liu wangyan , Zhu yinsu , Gu Kai , Wang jun , zhou xiaoyue , Xu yi , zhu xiaomei
Published: March 2022

Introduction: Cardiac magnetic resonance (CMR) is the gold standard for
evaluating myocardial fibrosis. Few studies have explored the
association between ventricular arrhythmias (VAs) and fibrosis in
apparently normal hearts. We aimed to investigate the association
between the occurrence and morphology of VAs and left ventricular late
gadolinium enhancement (LV-LGE) in patients without known structural
heart diseases. Methods: This study enrolled 78 patients with apparently
normal hearts who underwent simultaneous 24-h ambulatory Holter
electrocardiogram (ECG) and CMR examinations. The presence and extent of
LGE was determined using CMR imaging and compared based on occurrence
and morphology of VAs. The clinical characteristics were also recorded
and calculated. Results: LV-LGE was observed in 19 (37.3%) and 4
(14.8%) patients with and without VAs, respectively (P=0.039). It was
more frequently observed in patients with polymorphic VAs (P=0.024) and
ST-segment depression (P=0.001), and its extent was greater in
polymorphic VAs than monomorphic VAs, with a difference that approached
significance (P=0.055). In multivariate analyses adjusted for other
clinical variables, the presence of ST-segment depression (HR: 8.83;
95% CI: 2.23-35.50; P=0.002), drinking (HR: 6.84; 95% CI: 1.63-28.56;
P=0.008), and polymorphic VAs (HR: 25.24; 95% CI: 3.88-164.06; P=0.001)
were associated with greater prevalence of LV-LGE. Conclusion: In this
cohort of patients without structural heart diseases, myocardial
fibrosis was associated with multiple VA morphologies and ST-segment
depression on Holter ambulatory ECG measurements.
On generalized double almost statistical convergence of weight h
Authors: Savas Ekrem
Published: March 2022

n this work, our attention is focused on $\lambda$-
double almost statistical convergence of weight $h$. Mainly we work
$\lambda$- double almost statistical convergence of
weight $h$ and strong $\lambda$- double almost
summability of weight $h$.
Extracellular vesicle-derived microbiome obtained from exhaled breath condensate in patients with asthma
Authors: An Jin , McDowell Andrea , Kim Yoon-Keun , Kim Tae-Bum
Published: March 2022

BACKGROUND: Despite the construction of the metagenome of the asthmatic
lung, limitations persist in sampling the bronchial airway. This study
analyzed extracellular vesicles (EVs) obtained from exhaled breath
condensate (EBC) to compare the distinct characteristics of the
microbiome in asthmatics with those in healthy controls and proposed a
diagnostic artificial intelligence-based model of asthma. METHODS: We
obtained the EBC from 58 healthy controls and 251 patients with asthma.
EVs were isolated from the EBC and analyzed. The extracted 16s rDNA was
subjected to next generation sequencing. Taxonomic profiling was
conducted for all samples at the genus level. A combination of
artificial neural network (ANN) and gradient boosting (GBM) was applied
to selective EBC biomarkers. RESULTS: The asthma group exhibited
significantly higher alpha diversity based on the results of the Chao1,
Shannon, and Simpson indices. The bacterial composition of patients with
asthma different from that of the controls. At the genus level,
Sphingomonas, Akkermansia, Methylophaga, Acidocella, and Marinobacter
were significantly more abundant in patients with asthma. The diagnostic
model using GBM and ANN demonstrated good performance with respective
areas under the curve of 0.832 and 0.769. Firmicutes and Proteobacteria
at the phylum level were common important features between the GBM and
ANN asthma models. CONCLUSION: We demonstrated a distinct pattern in the
microbiome of patients with asthma, indicating the potential role of
microbiome-based diagnosis of asthma. To the best of our knowledge, this
was the first study to identify the microbiome in asthma using
EBC-derived EVs.
AtMC1 negatively regulates immunity through modulating pre-mRNA splicing and miRNA accumulation, involving LSM4
Authors: Wang Shune , Jiang Chunhao , He Chan , Shen Danyu , Zhang Haumeng , Zheng Ying , Zheng Liyu , Zhao Hongwei , Niu Dongdong
Published: March 2022

Alternative splicing (AS) of pre-mRNAs is an important gene regulatory
mechanism shaping the transcriptome. AtMC1 is an Arabidopsis thaliana
type I metacaspase that positively regulates hypersensitive response
(HR). Here, we found that AtMC1 is a negative regulator of plant
immunity to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst)
DC3000 and is physically associated with Sm-like4 (LSM4), which is
involved in pre-mRNA splicing. AtMC1 and LSM4 protein levels both
increased with their co-expression as compared with their separate
expression in vivo. Like AtMC1, LSM4 negatively regulates plant immunity
to Pst DC3000 infection. By RNA-seq, AtMC1 was shown to modulate the
splicing of many pre-mRNAs including 4CL3, which is a negative regulator
of plant immunity. Moreover, atmc1 mutant plants accumulated microRNAs
(miRNAs) such as miR398b, miR399a, miR165a and miR159a at a low level
than wild-type Arabidopsis Col-0 plants. Furthermore, AtMC1 affected
pri-miRNA processing. RNA immunoprecipitation revealed that AtMC1
interacted with these pri-miRNAs in vivo, suggesting that AtMC1 might
suppress their processing by directly binding to them. In addition, we
found that MC1-mediated plant immunity is conserved in tomato. Thus,
AtMC1 plays a regulatory role in both miRNA expression and pre-mRNA
splicing, which might contribute to AtMC1-mediated plant immunity.
Yesterday's Tradition, Tomorrow's Future -- Studying Subjective Hearing Loss in Older Adults Measured by Speech, Spatial, and Quality of Hearing Scale within the Framework of the ICF Core Set for Hearing Loss
Authors: ALFAKIR RAZAN
Published: March 2022

Objective: The utilization of the International Classification of
Functioning, Disability and Health (ICF) Brief Core Set for Hearing Loss
(BCS-HL) seems to be a promising tool to assess the factors underlying
SHL. This study aimed to assess categories underlying SHL in older
adults using the ICF-BCS-HL tool. Design: A cross-sectional study.
One-hundred and thirty-one independent-living older adults (Mage=72.32,
SD=6.83), who completed the speech, spatial, and quality of hearing
(SSQ) scale and a set of clinically accepted outcome measurements linked
with selected categories listed in the ICF BCS-HL. Results: A linear
regression analysis model was fitted with the outcome measurements after
controlling for age, sex, education, multimorbidity, and hearing aid
use. The model showed 5 significant predictors underlying the SSQ-total
score: HL, dizziness handicap, cognitive decline, multimorbidity, and
individuals’ ability to accept the noise level. Predictors varied across
the SSQ-subscales scores, however. While predictors underlying the
SSQ-Quality subscale scores were like the SSQ-total score, individuals’
ability to accept noise level was not one of the predictors underlying
the SSQ-Speech subscale scores. Whereas PTA, dizziness handicap and
multimorbidity were the predictors underlying the SSQ-Spatial subscale
scores. Conclusion: SHL, measured by SSQ, in older adults is a
multi-layer structure in nature and is associated with a range of
adverse health outcomes. A link of SHL with HL, cognitive deficits,
reduced ability to accept background noise level, and multimorbidity,
collectively or individually, were well-established. So far, little
attention has been paid to the impact of dizziness handicap of elderly
patients when evaluating their SHL.
An investigation of the present situation of labour analgesia in comprehensive Grade 3A hospitals in Fujian Province, China
Authors: Huang Yangfa , Huang Meifang , Zhang Liangcheng , Zhou Min , Wang Jing , Huang Qijian , Zeng Jian , Xu Xiaoping , Li Yumei , Liu Wenbin
Published: March 2022

Objective To investigate the present situation of labour analgesia in
comprehensive Grade 3A hospitals in Fujian Province, to analyse the
influencing factors, and to seek countermeasures to promote the
popularization of labour analgesia. Methods: The investigation was
conducted at all Western medicine comprehensive Grade 3A hospitals in
Fujian Province, field investigations and on-site questionnaire surveys
were adopted, and on-site interviews were conducted with leaders in
charge of the hospitals and leaders related to departments of labour
analgesia. Results: Among the 16 hospitals surveyed, 12 hospitals (75%)
routinely carried out intraspinal labour analgesia, and the analgesia
rates for 2015, 2016, 2017 and 2018 were 0.24%, 1.43%, 2.34% and
9.21%, respectively. At present, the quantity of labour analgesia is
insufficient, the proportion of analgesia is still low, and the
enthusiasm of hospitals is not high. Factors affecting the development
of labour analgesia mainly include a heavy workload/lack of staff
(93.75%), department coordination/leadership (87.50%), high cost/low
benefit (62.50%), maternal and family members (68.75%), and
insufficient publicity (68.75%). Conclusions: The labour analgesia work
of the comprehensive Grade 3A hospitals in Fujian Province is still in
its initial stage; there is still a lack of uniform implementation
standards and charging standards among hospitals in various cities. To
better promote labour analgesia, it is suggested that hospitals increase
the publicity of labour analgesia, strengthen the training of labour
analgesia personnel, standardize the implementation standard of labour
analgesia, set up appropriate charging items, and optimize labour
analgesia management while appropriately supplementing medical personnel
Investigation of Antimicrobial Effect of Fluoxetine in Experimental Rat Sepsis Model
Authors: Çakır Ahsen , Bozalı Kübra , Güler Eray , Şahan Ebru , Durdu Bülent , Sümbül Bilge , Koçyiğit Abdurrahim
Published: March 2022

Background and Purpose: Some studies showed that fluoxetine has some
promising properties in the treatment of specific infections; however,
its effects have not been studied in the sepsis model. This research
aims to investigate the effect of fluoxetine on the inflammatory process
in a sepsis model in rats and to investigate its efficacy in modifying
the antibiotic effect of imipenem. Experimental Approach: 40 rats were
equally divided into five groups. The first group is as a negative
control, group 2 is a positive control, group 3 treated with fluoxetine
5mg/kg, group 4 treated with Imipenem antibiotic 60mg/kg, and group 5
treated with fluoxetine combined with imipenem for 72 hours. The
expression level of serum and tissue HsCRP, pro-calcitonin (PCT),
lactate, myeloperoxidase activity (MPO), interleukin-1β (IL-1β),
interleukin-6 (IL-6), tumor necrosis factor (TNFα), and monocyte
chemoattractant protein-1 (MCP-1) was measured using ELISA. Oxidative
stress markers were measured using photometric methods, total thiol
(TT), native thiol (NT), total oxidant status (TOS), and total
antioxidant status (TAS). Total tissue protein concentrations were
measured by the Bradford method. Key Results: In fluoxetine, imipenem,
and combined (fluoxetine + imipenem) groups, the IL-1β, IL-6, TNF-α,
MPO, MCP-1, HsCRP, PCT, lactate, TOS, OSI, and disulfide levels were
reduced (p<0.05). The antioxidant indicator (TT, NT, and TAS)
levels significantly increased (p<0.05). Fluoxetine and
imipenem combined therapy showed positive synergistic effects.
Conclusion and Implications: This research shows that fluoxetine has an
anti-inflammatory and antioxidant effect and its combined therapy with
imipenem shows positive synergistic effects in the experimental sepsis
model.
CFD simulation of gas--liquid rotational and perforated concurrent flows on the blade unit of a TRST
Authors: Wang Hongkai , Tang Meng , Wang Dewu , Liu Yan , Yan Fei , Wang Ruojin , Zhang Shaofeng
Published: March 2022

The blade unit of a tridimensional rotational flow sieve tray (TRST) is
the origin for the rotational–perforated flow. The flow field of that
was investigated using the Multi-fluid VOF method. The water fractions,
and the gas perforation velocity on the blade unit were investigated.
The liquid phase distribution in the jet and mixed flow is more
dispersed than that in film and jet flow. The gas–liquid two-phase
velocity fluctuated with the radial distance. The rotational and
perforated flows on the blade unit were analyzed separately by the local
rotational flow ratio . The variation range of the ratio for the
gas-phase was 0.6 – 0.85, and that for the liquid-phase is 0.2 – 0.8.
Based on the Q criterion, the vortices were mainly concentrated near the
sieve holes. The simulation provides an important theoretical basis for
the mass transfer investigation and structural optimization of the blade
unit.
The Efficacy and Safety of Baduanjin in Patients with Coronary Heart Disease After Percutaneous Coronary Intervention：A Systematic Review and Meta-analysis
Authors: Li Xing , Dai hua , Tan huan , Zhang ling
Published: March 2022

Objective: To evaluate the effects of Baduanjin on quality of life
(QOL), psychological status and cardiac function of patients with
coronary heart disease after percutaneous coronary intervention (PCI).
Methods: PubMed, The Cochrance Library, Embase, Web of Science, China
Biology Medicine disc, CNKI, VIP and Wanfang were searched by a computer
for any randomized controlled trials of Baduanjin intervention in
patients with coronary heart disease after PCI, including relevant
conference articles and academic papers published in journals. The
retrieval time was from the database creation time to September 7, 2020.
After two evaluators independently screened the literature, extracted
relevant data and evaluated the bias risk for the included studies,
RevMan5.3 software was used for a data analysis. Results:A total of 9
articles and 846 patients were included. The meta-analysis showed that
the Baduanjin group was superior to the conventional western medicine
treatment group in physical hunctioning、role-physical 、general
health、role-emotional 、mental health、 anxiety、 anxiety、left
ventricular ejection fraction、blood pressure、stroke volume，and the
differences were statistically significant.But the Baduanjin group
was not superior to the control group in bodily pain、vitality、social
functioning,and the differences were not statistically significant.
Conclusion: Baduanjin can improve the cardiac function, regulate the
psychological state and improve the QOL among patients with coronary
heart disease after PCI.
Chlorine-resistant positively charged nanofiltration membranes formed by SI-ATRP method for heavy metal ions removal
Authors: Wu Bin , Weng Xiao-Dan , Wang Naixin , Yin Ming-Jie , Zhang Lin , An Quan-Fu
Published: March 2022

Heavy metal pollution is one of the most serious environmental problems.
Nanofiltration is an effective and potential membrane separation
technology for removal of heavy metal ions from water. However, the
separation performance and chlorine resistance of the nanofiltration
membrane should be further improved in industry. In this study,
positively charged polyamide (PA-PDMC) nanofiltration membranes were
fabricated to remove heavy metal ions from water. The obtained membrane
had high flux and chlorine resistance by grafting
methacryloxyethyltrimethyl ammonium chloride (DMC) on the surface of
2-bromoisobutyryl bromide (BIBB) immobilized polyamide (PA-Br1)
membranes via surface-initiated atom transfer radical polymerization
(SI-ATRP). The retentions to divalent cations of the as-prepared PA-PDMC
membrane was above 90% with the flux of 82.5 L m-2 h-1. Furthermore,
the PA-PDMC membrane showed a stable separation performance during a
long-time filtration process of 168 h, which exhibited an exceptional
chlorine resistance.
Kerr-(A)Ds, Myers–Perr black-hole Galilean ον gravitational transformations for the anti-COVID-19 RoccuffirnaTM evolutionary drug design.
Authors: Grigoriadis Ioannis
Published: March 2022

It is thought that all of the rich content in the present-day Universe
based on an array of recent observations developed through gravitational
amplification of primeval density fluctuations generated in the very
early phase of cosmic evolution. In this paper, we strongly combine
machine learning characteristics to achieve very high accuracy levels
for the in-silico generation of the RoccuffirnaTM small molecule, a
ligand targeted the SARS-COV-2 virus main protease (M pro ) using
Quantum Kerr-(A)dS and Myers–Perry black microBlackHole-Inspired
Gravitational for both Euclidean and Lorentzian signatures in Practice.
We provide also an extensive toolbox of methods for performing quantum
schrodinger inspired docking algorithms, teleportation and other
information-theoretic tasks in MathCast programming language, and
compared these algorithms by means of mean percentile free energy
ranking, in a new recall-based evaluation metric for the in-silico
design of the Novel Series of the RoccuffirnTMQMMMCoRoNNARRFr
anti-(nCoV-19) ligands. In this paper we in-silico designed new drug
leads that target the COVID-19 virus main protease (M pro ). M pro, a
key CoV enzyme, which plays a pivotal role in mediating viral
replication and transcription, and discuss various general results
including Galilean transformation to a rigid QMMM heuristic horizon
topology, and near-horizon fragmentation symmetry ranging from
supergravity theories to enhance the Roccuffirna’s gravity to trap the
SARS-COV-2 viruses in practice.
Digital solutions for color vision deficiency : Systematic review
Authors: S Meenakshi , Singla Anshu
Published: March 2022

Color perception plays a vital role in what human beings perceive and
react to. Color vision deficiency (CVD) or Color blindness is a key
anomaly in color perception. Advancements in digital solutions resulted
in developing tools for CVD. Analysis of existing literature indicate
several simulation and recoloring algorithms which assume complete
deficiency with focus on protanopia and deuteranopia. The authors
started with elucidating the research questions involved, stratified the
digital solutions and benchmarks employed for their effectiveness till
date. Since only the subjective evaluation of solutions existed, the
authors proposed an index called the Colorblind descriptive quality for
benchmarking them.
Green synthesis of S-doped carbon nanotubes and their application as conductive additive in Li ion batteries
Authors: Zhao Lu , Ning Guoqing , Zhang Shaochun
Published: March 2022

Sulfur-doped carbon nanotubes (SCNTs) exhibit better conductivity and
hydrophilicity, as compared to the undoped carbon nanotubes (CNTs). Low
cost mass production of SCNTs is highly desirable for their real
application at an industrial scale. Here, scalable green syntheses of
SCNTs and S, N dual doped CNTs (SNCNTs) are demonstrated based on the
gaseous S doping using SO2 as dopant. The process not only significantly
promotes the hydrophilicity and conductivity of CNTs due to the
incorporation of S atoms in carbon framework, but also purifies the
final product due to burning off the carbon coatings of metal particles.
SO2 is generated by CaSO3 pyrolysis and absorbed by CaO, thus realize
the recycling of S species. The LiFePO4 cathodes using the as-prepared
SCNTs and SNCNTs as conductive additive exhibit enhanced capacity and
rate performance than the electrodes prepared with the pristine CNTs,
not matter using N-methyl pyrrolidone or water as solvent.
ACUTE GVHD TRIGGERED BY COVID-19 IN A BONE MARROW RECIPIENT
Authors: Gözmen Salih , devrim ilker
Published: March 2022

Introduction There are few reports regarding risk of COVID-19-associated
complications in bone marrow recipients. We report a patient who had
graft-versus-host disease triggered by COVID-19. Results The patient was
a 17-year-old boy who underwent HSCT from matched sibling donor for CML.
He had complete donor chimerism at day +30. Cyclosporine was tapered and
stopped at day +65. Until day +93, he had no complaints. The patient
started to suffer from cutaneus and gastro-intestinal grade III GVHD
after the diagnossis of CIVID-19. Discussion In the absence of any other
condition to explain this clinical picture and the good response to GVHD
treatment indicate that the triggering factor in the development of GVHD
may be COVID-19 in the era of TLR, which plays a common role in the
pathogenesis of both GVHD and COVID-19.
Surface expression of the immunotherapeutic target GD2 in osteosarcoma depends on cell confluency
Authors: Wiebel Malena , Kailayangiri Sareetha , Altvater Bianca , Meltzer Jutta , Grobe Kay , Kupich Sabine , Rössig Claudia
Published: March 2022

Background: Chimeric antigen receptor (CAR) T cell therapy of pediatric
sarcomas is challenged by the paucity of targetable cell surface
antigens. A candidate target in osteosarcoma (OS) is the ganglioside
GD2, but heterogeneous expression limits its value. We aimed to identify
mechanisms that upregulate GD2 expression in OS. Methods: Surface
expression of GD2 in OS cell lines was assessed by flow cytometry.
Transcription and translation of genes encoding for enzymes involved in
GD2 synthesis were studied by quantitative real-time PCR and by using a
construct containing the 5’ untranslated region (5’UTR) of the GD3
synthase-mRNA. An in vitro cytotoxicity assay was used to study the
sensitivity of OS cells to targeting by GD2-specific CAR T cells.
Results: GD2 surface expression varies both among and within individual
OS cell lines. Pharmacological approaches, including inhibition of the
histone methyltransferase Enhancer of Zeste Homolog 2 (EZH2) and
modulation of the protein kinase C (PKC), failed to increase GD2
expression. Instead, cell confluency was associated with higher GD2
expression levels both in monolayer cultures and in tumor spheroids and
with increased sensitivity to in vitro cytolysis by GD2-specific CAR T
cells. Confluency-dependent upregulation of GD2 expression in OS cells
is mediated by increased de novo biosynthesis through a yet unknown
mechanism. Conclusions: Expression of GD2 in OS is highly variable and
associated with increasing cell confluency in vitro. Strategies for
selective upregulation of GD2 are needed to enable effective therapeutic
targeting of this antigen in OS.
Mechanism of immune tolerance induced by Immutol combined with CsA in rat cardiac transplantation
Authors: Ma Jun , Zhang Xinxue , Liu Zhe , Liu Huanye , Yang Long , Kou Jiantao , Li Xianliang , He Qiang
Published: March 2022

Backgrounds: Indoleamine 2,3-dioxygenase (IDO) has previously been
elucidated in tolerant cardiac or liver transplantation models.
Kynurenine has also been reported in immune tolerant models. Immutol is
an analogue of IDO and metabolites, and it is an anthranilic acid
derivative and is a synthetic chemical. Its function in tolerant rat
cardiac transplantation remains unknown. Here, we elucidated the role
and mechanism of Immutol combine with CsA in tolerant cardiac
transplantation. Methods: Using a rat cardiac transplantation model
intervented by Immutol, CsA, and Immutol+CsA before surgery. Moreover,
we used 1-MT, the IDO inhibitory, to furtherly validate the role and the
mechanism in the immune tolerance of cardiac transplantation Results: In
this study, we show that the combination of Immutol with CsA can
alleviate rejection and prolong recipients’ survival compared with that
in the Immutol or CsA groups. Moreover, the protein level of Kynurenine
was high overexpression in the Immutol+CsA group, and the expression
level of IDO and Kynurenine was increased with survival time longer in
the Immutol+CsA group. Furthermore, we used 1-MT to detect the role of
Immutol in the rat cardiac transplantation. The results revealed that
rejection was aggravated, and survival time was shortened after the IDO
was successfully blocked. Conclusion: Our data suggest that the
combination of Immutol and CsA can alleviate acute rejection and
prolongs survival time in a rat cardiac transplantation model; this drug
may induce immune tolerance in cardiac transplantation through
‘kynurenine accumulation’, which may provide new ideas and methods for
clinical induction of transplant immune tolerance
Total antioxidant capacity and oxidative stress in follicular fluid and embryo cell culture media, their role in fertilization and embryo selection in IVF cycles
Authors: seyfettinoglu sevtap , Sahin gulnaz , Akdogan Aysin , Tavmergen Ege Nazan , Akcay Yasemin , Sozmen Eser , Tavmergen Erol
Published: March 2022

Objective: The aim of this study is to evaluate antioxidant parameters
as well as levels of lipid peroxidation, paraoxonase enzyme, and
nitrotyrosine in the follicular fluid and embryo culture media among
patients under IVF treatment. Material and methods: Fifty-one patients
who underwent IVF / ICSI were enrolled in this prospective study at the
IVF Center of University Hospital. A long agonist protocol was used in
22 cases for controlled ovarian stimulation, and an antagonist protocol
was used in 29 cases. Two-hundred sixty follicular fluid samples from a
total of 454 follicular fluids obtained from the cases and cell culture
media of 51 transferred embryos (one from each case) were examined.
Follicle fluids and embryo cell culture media were divided into groups
according to oocyte fertilization, the protocol applied, the day of
transfer, the quality of the embryos obtained and whether pregnancy was
achieved. medium obtained from 51 individually cultured embryos and
investigating their relationship with the controlled ovarian stimulation
applied Results: Thiobarbiturate reactive substances (TBARS) levels were
significantly lower in the fertilized oocytes follicular fluids compared
to the unfertilized ones (21 vs. 29.75 nmol/ml, p = 0.002). There were
no differences with regard to other parameters. Trolox Equivalent
Antioxidant Capacity (TEAC) levels were higher both in the fertilized
and unfertilized oocytes in the agonist protocol group compared to the
antagonist protocol group. As to the quality of embryos, there were no
differences between the groups in terms of the investigated parameters
and between the pregnant and the nonpregnant groups in follicular fluid
analysis. TEAC levels were higher in the embryo cell culture media in
the pregnant group during the day 2 embryo transfer. we found no
association between follicular fluid TEAC levels and fertilization rates
in total, however, TBARS values were significantly higher in the
unfertilized group, suggesting that
Promoting Practitioner Wellness in the Neurotology Clinic -- An Ergonomic Assessment of the Dix-Hallpike's Test.
Authors: Nair Sanjay , Ammari Tareq , Gohil Rohit , Bennett Alex
Published: March 2022

Objectives: Numerous tasks carried out in the otolaryngology clinic
increase the chances of developing injury among practitioners. To
mitigate this, we aim to observe the risk of musculoskeletal injury in
practitioners when carrying out common procedures. We observe the
performance of the Dix-Hallpike manoeuvre, deducing whether technique
has an impact on the likelihood of developing injury. Design:
Participants were asked to perform the Dix-Hallpike manoeuvre as they
normally would in clinic, on departmental colleagues. Data that was
collected included chosen preference of position when carrying out
manoeuvre, level of seniority of practitioner and risk of
musculoskeletal injury to practitioner. Setting: Simulated outpatient
department; St John’s Hospital, Edinburgh. Participants: Otolaryngology
consultants and trainees with clinic commitments, having regularly
performed the Dix-Hallpike manoeuvre. Main outcome measures: Risk of
musculoskeletal injury was measured using the validated Rapid Upper Limb
Assessment (RULA) tool. Results: 3 consultants and 7 trainees were
included in this study performing on average 4 Dix-Hallpike’s during the
study period – totalling 40 attempts. The median RULA score was 4 for
the sitting position, compared to 6 for standing (p<0.0001).
There was similar statistical significance when consultants and trainees
were evaluated separately. Conclusions: A doctor’s wellness is of great
importance to facilitate long-term job satisfaction and productivity. It
is important to improve conditions for the practitioner in the otology
clinic, and one factor is procedural technique. Further work is needed
to raise ergonomic awareness amongst otolaryngology surgeons.
A numerical scheme based on the collocation and optimization methods for accurate solution of sensitive boundary value problems
Authors: MEHRPOUYA MOHAMMAD ALI , SALEHI REZVAN
Published: March 2022

Despite the significant advances in the numerical solution of nonlinear
boundary value problems, most of the existing methods still encounter
with a high sensitivity to the initial guess. The aim of this paper is
to propose a less sensitive robust numerical scheme for accurate
solution of sensitive boundary value problems. For this purpose, an
orthogonal collocation approach for discretization of the problem is
utilized. Thereby, the problem is converted to the solution of nonlinear
algebraic equations. However, due to the difficulties of solving the
obtained system of nonlinear equations, particularly in providing the
proper initial guess, the obtained system of equations is transferred to
an optimization problem in which the values of the solution at
collocation points are considered as decision parameters. The method
finds good results even using not good initial guess for decision
parameters and even using a small number of discretization points.
Numerical results of two benchmark examples are presented and the
efficiency of the method is reported.
On the Blow-up criterion of Navier-Stokes equation associated with the Weinstein operator
Authors: Bettaibi Youssef
Published: March 2022

In this paper we give Navier-Stokes system associated with the Weinstein
operator $(NSW)$ (see.\eqref{11}), We study the
existence and uniqueness of solutions to equations (NSW) in
$L_{\alpha}^{p}\left(\mathbb{R}_{+}^{d+1}\right),
2 \alpha+d+2
Success rate of tympanoplasty in chronic suppurative otitis media patients: A retrospective study
Authors: hassen seid , Fröschl, Uta , yesuf subah , alamdo andamlak
Published: March 2022

Abstract Objectives: This study aimed to determine success rate of
tympanoplasty in CSOM patients & its prognosticating factors. Design
and setting; A facility based retrospective cross sectional study design
was employed to assess the characteristics of chronic suppurative otitis
media patients and the success rate of tympanoplasty of patient who
underwent surgery at ORL-HNS of SPHMMC, Addis Ababa, Ethiopia. Methods:
Ninety patients who had undergone tympanoplasty in the year 2019 from a
retrospective database were studied. Data was collected using a
structured checklist and analysed using SPSS 25. Baseline profiles of
participants were computed using descriptive statistics. Paired t-test
was used to compare preoperative and postoperative ABG results. Multiple
regression analyses of potential factors (intra-& pre-operative
characteristics) were used to identify predictors for success of
tympanoplasty while the level of significance was set at
p<0.05. Anatomical success was defined as a complete
integration of the graft and functional success as an ABG closure of
≤20dB after 6 months postoperatvely. Results: The postoperative
anatomical & functional success rates for tympanoplasty were 81.1% and
60%, respectively. According to the statistical results of the study,
neither the anatomic success nor the functional was found to be
prognosticated by the potential factors analyzed. Conclusion:
Tympanoplasty is an effective surgical procedure that can result in
improved auditory function in patients and prevents complications
satisfactorily irrespective of baseline characteristics. With
tympanoplasty, the ear heals and the patient hears. Keywords:
Tympanoplasty; CSOM; success rate; SPHMMC Succinct points: Endaural
Tympanoplasty; temporalis fascia graft; air bone gap in dB; endoscopic
tympanoplasty; 6months follow up period
Predictive index of plastic bronchitis in children with refractory Mycoplasma pneumonia
Authors: zhu zhenli , xu yongsheng
Published: March 2022

Abstract Objective To explore the predictive indicators of plastic
bronchitis (PB) in children with refractory Mycoplasma pneumoniae
pneumonia (RMPP). Methods Retrospective analysis of children who were
hospitalized in Tianjin Children’s Hospital from December 2018 to
January 2020 who met the RMPP diagnostic criteria and were treated with
bronchoscopy and alveolar lavage. Collect and compare the clinical
medical records of the two groups of children, and use Logistic
regression analysis to find the predictive indicators for the occurrence
of PB in children with RMPP. Results ①The incidence of hypoxemia in
group II was higher, the days of fever was longer, hospital stay was
longer, and radiological manifestations were more serious
(P<0.05).②The use rate of gamma globulin in group II was
higher, and the hormone dose was higher (P<0.05).③The levels
of WBC, CRP, LDH, FER, D-D dimer, PLT, PCT, IL-6, AST and percentage of
neutrophils in peripheral blood in group II were higher than those in
group I (P<0.05). The percentage of lymphocytes in group II
was lower than that in group I (P <0.05). ④The ROC curve and
Logistic regression analysis showed that the days of fever, N%, CRP and
LDH were independent risk factors for the occurrence of PB in children
with RMPP. The cut-off values of days of fever, N%, CRP and LDH were
11.5 days, 68.4%, 45.5 mg/L and 559U/L respectively. Conclusions The
days of fever≥11.5days, N%>68.4%,
CRP>45.5mg/L, LDH>559U/L are predictors of PB
in children with RMPP.
Is Helicobacter pylori Infection a Driver in Pregnancy Related Disorders: A Review
Authors: Hollamby Henry , Allan Elaine
Published: March 2022

Helicobacter pylori is a successful pathogen, infecting approximately
60% of the world’s population (1). It causes numerous gastrointestinal
conditions, and was named a class I carcinogen by the World Health
Organisation (WHO) in 1994 (2). This paper reviews studies assessing the
impact of H. pylori on pregnancy related disorders such as Hyperemesis
Gravidarum, Iron deficiency anaemia and fetal growth restriction and
examines the proposed pathogenic mechanisms by which H. pylori is
thought to cause these pregnancy related disorders through the use of
virulence factors and specialisms it possesses.
Transcriptomic Profiling of Human Placenta in Gestational Diabetes Mellitus at the Single-Cell Level
Authors: yang yuqi , guo fang , peng yue , chen rong , zhou wenbai , wang huihui , ouyang jun , yu bin , xu zhengfeng
Published: March 2022

Objective To generate a comprehensive transcriptomic profile of cellular
signifures and transcriptomes of human placenta in GDM by single-cell
RNA-sequencing (scRNA-seq), and built a comprehensive cell atlas. We
hope to reveal the molecular mechanism of pregnancy risk for GDM women.
Design Scientific study. Setting University Hospital and laboratories.
Population or Sample Pregnant women Methods 20 GDM women and 20 normal
pregnant women were recruited in present study. ScRNA-seq were loaded on
the Chromium Single Cell Controller Instrument (10×Genomics) to generate
single cell gel beads in emulsions (GEMs). Results A total of 27,220
cells from placenta samples were obtained. First, with the
cell-type-specific marker genes, we annotated 15 cell clusters into more
than 9 different cell types. Second, beside the classcial markers, we
also found some novel markers for distinguishing three kinds of
trophoblast and subtypes, which cloud be confirmed by
immunohistochemistry imaging. Third, we demonstrated the specific
placental function in GDM by the bioinformatics analysis of
differentially expressed genes, such as estrogen signaling pathway,
natural killer cell mediated cytotoxicity down- regulated. Fourth, there
are abundant ligand-receptor interactions between trophoblast and immune
cells in the maternal fetal interface microenvironment, such as
VEGFB-FLT1, MIF-EGFR, RPS19-C5AR1, SPP1-CD44These dysfunctional
ligand-receptor interactions may play the important roles in the
development of GDM. Conclusion This study provided the first
cell-type-specific transcriptomic alterations GDM placenta from single
cell level, exploded the cell identities and cell-type-specific marker
genes in the human placenta. In addition, it demonstrated the features
of placental function and cell interactions for GDM.
Management of hypertensive disorders in pregnancy in Black women: a systematic review
Authors: John Jeeva , Curry Gwenetta
Published: March 2022

Black women are four times more likely to die than White women in
pregnancy in the U.K. Black women are more prone to Hypertensive
Disorders in Pregnancy (HDP). Outside of pregnancy, there are race based
differences in the management of hypertension as Calcium-Channel
Blockers (CCB) are more effective in reducing blood pressure in Black
patients. It is unclear whether these differences in anti-hypertensive
management extend to the management of hypertension in pregnancy.
Objectives The primary objective was to address this gap in evidence by
undertaking a systematic review of all randomised control trials
investigating pharmacological management of HDP to assess whether CCBs
are the most effective anti-hypertensive agent in Black pregnant women.
Search Strategy The following electronic databases were searched:
PubMed, MEDLINE and Embase. We used MeSH and free text terms in
conjunction to increase sensitivity to potentially relevant studies.
Selection Criteria Inclusion criteria: (1) study involved treatment of
HDP; (2) study was of a randomised control trial design; (3) one of the
treatment arms involved CCBs and (4) English full-text. Data collection
and Analysis Information regarding baseline participant data, type of
anti-hypertensive, and clinical outcomes was extracted from each study.
Main results This current review highlighted one randomised control
trial, which stratified HDP outcomes by ethnicity. Conclusions There is
a lack of evidence to draw conclusions as to whether CCBs are the most
effective anti-hypertensive agent for Black patients with HDP. Funding
None Keywords Hypertension; pregnancy; pre-eclampsia; ethnicity;
anti-hypertensive; Calcium-Channel Blocker; race; Black; maternal
mortality; maternal morbidity
Synthesis, DFT Calculations, Vibrational Assignments (FT-IR & FT-Raman) and Hirshfeld Surface Analysis of 3-(2-chloroethyl)-2,6-diphenylpiperidin-4-one
Authors: Krishnamoorthy Rajkumar , Sambandam Sivakumar , RAMALINGAM Arulraj , Kumar Anitha
Published: March 2022

The optimized molecular structure, vibrational frequencies and
corresponding vibrational as-signments of
3-(2-chloroethyl)-2,6-diphenylpiperidin-4-one [CEDP] have been
investigated ex-perimentally and theoretically based on Density
Functional Theory approach. The CEDP com-pound crystallizes in the P21/c
space group with one independent molecule in the asymmetric unit. The
piperidine ring adopts a chair conformation with puckering parameters of
Q = 0.576 (2) Å, θ = 164.2 (2°), φ = 179.4 (8°). The substituents on the
piperidine ring adopt equatorial orienta-tions with the keto oxygen atom
being anti-clinal O1‒C3–C4–C5 = 136.1 (2)°. The FT-IR and FT-Raman
vibrational spectra of CEDP were recorded in the solid phase. The
intermolecular in-teractions and electron density of the crystal
structure were analysed using Hirshfeld surface and fingerprint
analysis. The occurrence of strong intermolecular hydrogen bonding was
confirmed by Natural bond orbital analysis.