bluuebunny/crossref_metadata_2025 huggingface dataset:
Compliance to modification of risk factors for recurrent myocardial infarction in the long term of the disease: a randomized study
Authors: Sitovskyi Andrii , Andriichuk Olga , Yakobson Olena , Ulianytska Nataliia , Usova Oksana
Published: June 2023

Purpose: to study the relationship between awareness and commitment to modifying risk factors for recurrent myocardial infarction in a long period of the disease.

Material & Methods: to study the awareness of risk factors and adherence to secondary prevention of myocardial infarction (MI), a representative sample was created. Of 912 patients treated for myocardial infarction, 333 patients were randomized, taking into account proportional distribution in the population by age (under 65 and over 65 years) and gender. The average age of patients was 62.5±9.8 years: 70,27% of men and 29,73% of women.

Patients' awareness of risk factors, the use of preventive measures and adherence to treatment were assessed on the basis of a questionnaire.

Statistical processing of the obtained material was carried out using the statistical program STATISTICA 12.5 (StatSoft.Inc). Results are presented as mean and standard deviation (M±s), number of options (n). To compare the qualitative characteristics (frequency tables), the χ2 test and Fisher's exact test were used.

All participants were informed about the aims of the study and gave written consent to participate in the study.

Results: low awareness (Aw) and commitment (Cm) to the modification of such risk factors for recurrent infarction in a long period of the disease as: reduced fat intake Aw 16,52%, Cm 19,4%; regular consumption of vegetables and fruits Aw 11,41%, Cm 9,7%; smoking cessation Aw 15,92%, Cm 11,04%; decrease in alcohol consumption Aw 16,52%, Cm 10,70%; increased physical activity Aw 14,41%, Cm 12,37%; weight loss with its excess Aw 3,0%, Cm 2,34%; avoidance of stress Aw 28,53%, Cm 18,06%. Although the vast majority of patients received appropriate recommendations from the doctor.

Conclusions: low awareness of the possibility of modifying heart disease risk factors is associated with the failure to implement such non-pharmacological measures in secondary prevention after MI: decrease in fat intake (χ2=65,12; p=0,000) and regular consumption of vegetables and fruits (F p=0,000), unwillingness to quit smoking (F p=0,000) and decrease in alcohol consumption (F p=0,000), unwillingness to increase physical activity (χ2=17,61; p=0,000) and reduce weight in case of its excess (F p=0,015), avoiding stress (χ2=27,42; p=0,000).
Screening and Optimization Consortium Technique of the Bacillus megatherium and Saccharomyces cerevisiae Microbial Consortium for Ethanol Detection
Authors: Nurdiani Nurdiani , Iswantini Dyah , Nurhidayat Novik , Wahyuni Wulan Tri , Kartono Agus
Published: June 2023

Determination of alcohol content is very important in the food and beverage industry. Biosensor is an alternative to measuring ethanol content. Alcohol biosensors with a single microbe still have a narrow measurement area at ethanol concentrations, so a microbial consortium is needed to widen the range of measured ethanol concentrations. Therefore, it is necessary to screen the microbes from Bacillus sp and S. cerevisiae which have the potential to produce alcohol dehydrogenase (ADH) enzymes and optimize the consortium technique that can provide the best response to oxidation currents. In the yeast microbial screening of 14 S. cerevisiae isolates and 5 Bacillus sp isolates, it can be concluded that for the 14 yeast S. cerevisiae isolates that have the potential to produce ADH enzymes, there is one isolate with the SCRF code. For the 5 bacterial isolates of Bacillus sp that have the potential to produce ADH enzymes, all Bacillus isolates with the code Bacillus megatherium 29/9/14, Bacillus megatherium 23/6/22, Bacillus 6, Bacillus 53, and Bacillus 55. Based on the oxidation current data, Bacillus megatherium 23 /6/22 produces the highest current compared to other Bacillus isolates. The consortium technique that provides the highest current is the method of mixing 1:1 (µL) microbial suspension in an Eppendorf container. Optimization of the consortium's biofilms using the Response Surface Method was produced at 10 days of age, pH 7.5, and 75 µL of microbial suspension dripping volume.
Characterization Study of Dyes Photodegradation-Adsorption Products by TiO2-Chitosan Immobilized on Glass Beads using Flow System
Authors: Mahadika Bella Sukma , Saefumillah Asep , Saepurahman Saepurahman , Kurnia Kiki Adi , Wellia Diana Vanda
Published: June 2023

Dyes have a complex molecular structure that is difficult to degrade and can persist for a long time in water bodies. This study examines more thoroughly the effectiveness of photodegradation-adsorption of dyes, kinetic aspects, isotherms, thermodynamics, identification of the structure of photodegradation products, and changes in environmental quality parameter values whose results are quite satisfactory. The combination of photodegradation-adsorption of dyes by TiO2-chitosan modified glass beads using a flow system has never been accomplished before. Flowing of the dye resulted in a dye degradation efficiency of 99%. The FESEM-EDX analysis shows that TiO2-chitosan has been coated onto the surface of the glass beads. R2 of kinetics MB, MO, RB are 0.99234, 0.9889, 0.9971, which were pseudo-first order. Qm, KL, R2 are 6.4382, 0.70533, 0.99923; 16.7364, 1.42059, 0.98816; 6.0078, 1.06973, 0.99889, respectively. The ∆H, ∆S, ∆S of -9.388, -0.024, -1.979; -27.182, -0.078, -3.592; -4.819, -0.017, 0.464 indicate the exothermic and spontaneous reaction. COD, TOC, pH, turbidity values have reached the threshold required by the government. The degradation products formed during the photodegradation-adsorption process were identified using LC-MS that the structure of the dye has been degraded to become simpler so that it is safely disposed of into the environment.
Semiótica de las figuras que dan la espalda al espectador: relaciones entre literatura, pintura y realidad
Authors: Parellada-Bezares Cristina
Published: June 2023

Este artículo aísla metódicamente las figuras que en la pintura dan la espalda al espectador a partir del Trecento. Son motivos minoritarios, pero semánticamente fértiles, que renuevan los esquemas iconográficos y generan un nuevo orden visual y, por ello, son un caso de estudio paradigmático para explicar algunos de los fundamentos y problemáticas de la estrategia semiótica de análisis de la imagen. La semiótica visual estudia las figuras dorsales como signos abiertos y proxémicos que tienen en la vida referentes culturales y sociales, y referencias lingüísticas y conceptuales en la literatura, pero también producen significados desde su propio medio pictórico porque las imágenes denotan y connotan con autonomía de sentido.
Hannah Arendt y Michel Foucault: Un pensamiento asistemático y sismográfico
Authors: Toscano López Daniel
Published: June 2023

Este artículo analiza el carácter asistemático de Arendt y Foucault, el cual, lejos de denotar falta de rigurosidad, se erige en un modo particular de investigación que adopta la forma de un laboratorio de pensamiento en el que ambos son como “sismógrafos” que, al conjugar la reflexión filosófica junto con el análisis histórico, detectan, por una parte, el agotamiento de conceptos que de tanto ser usados por la tradición de la filosofía política entran en desuso y, por otra, captan puntos frágiles tanto en nuestras costumbres nocivas como en nuestros hábitos de pensar, actuar y sentir. Para llevar a buen puerto lo anterior: primero se examinan dos prejuicios que pueden erigirse frente a Arendt y Foucault y que son fruto de los particulares estilos de investigación de estos autores. Luego, se abordan en tópicos separados tanto el estilo asistemático como el modo histórico, específico y contingente con el cual Arendt y Foucault acometen sus respectivas indagaciones, el cual se ve reflejado en la “separación y discriminación afinada” de conceptos y problemas abordados por ellos. Finalmente, se formula el asunto de la verdad en ambos, así como el singular modo en que lo enfrentan: en Arendt la verdad ligada a la responsabilidad personal y en Foucault articulada con la producción de subjetividad.
CRAAP not crap
Authors: Grundtke-Gräfin von Westarp Lisa
Published: June 2023

Während der Literaturrecherche für Haus-, Bachelor- und Masterarbeiten, aber auch im privaten Umfeld, hat man es heutzutage mit einer Vielzahl von Informationsquellen unterschiedlicher Qualität zu tun. Gleichzeitig ist es schwer alle Quellen auf Authentizität und Seriosität beurteilen zu können. Dabei kann der CRAAP-Test helfen. Es gibt Schemata und Kriterien, anhand derer sich die Seriosität einer Quelle schnell beurteilen lässt. Mit oder ohne Schemata, einen kritischen Blick gegenüber Informationen sollte man immer behalten.
Problemáticas de la Biblioteca Nacional de México para ejercer como Órgano Bibliográfico Nacional
Authors: Waybel Sánchez Eduardo
Published: June 2023

Las bibliotecas nacionales son instituciones que tienen la misión de organizar, resguardar y difundir el patrimonio bibliográfico y documental de una nación. En el caso particular de la Biblioteca Nacional de México (BNM), el pertenecer a la Universidad Nacional Autónoma de México (UNAM) le impide desempeñar algunas de sus responsabilidades básicas y destacar como un sistema de servicio bibliotecario por excelencia y de nivel nacional. El presente trabajo tiene como objetivo analizar la problemática y los motivos por los que la BNM no ejerce en su totalidad las funciones del Órgano Bibliográfico Nacional. A través del método de análisis documental y la técnica de revisión y selección de documentos, se anuncian los desafíos a los que se enfrenta la biblioteca para el cumplimiento de dichas normativas y leyes, como ejemplo, velar por la Ley del Depósito Legal o administrar las oficinas del ISBN e ISSN. Asimismo, se analizan algunas opiniones sobre dicha problemática externadas por algunos autores del ámbito bibliotecológico y se discute una postura frente a la disyuntiva de que la BNM sea administrada por el Estado o que siga siendo parte de la UNAM, aunque con la implementación de algunas medidas que mejoren el cumplimiento de sus obligaciones.
The use of Design Thinking Method in applied research to integrate the library in distance learning platforms with a learning object
Authors: Centeno Alayón Purísima
Published: June 2023

This work aimed to integrate the most in-demand library services and resources in distance education platforms through the creation of a learning object for the Natural Sciences Faculty, at the University of Puerto Rico, Río Piedras Campus.  For this object, the Design Thinking Method was applied, which consists of the following steps: empathizing, defining, ideating, prototyping, and testing.  The research approach was mixed.  Some information was gathered from participatory observation and netnography, as qualitative methodologies, to identify the needs of the academic community.  In addition, the visits statistics to the Centro de Información y Tecnología web pages were considered from the quantitative perspective to contrast them with the findings of the qualitative part.  The study found that the resources and services with the greatest demand were journals, databases, remote access, and interlibrary loan.  Identifying needs early on helps design solutions that satisfy the user.  When applying the Design Thinking Method and, in its first step, empathy considered the library services needs from the beginning.  This helped the rest of the steps of the method to be responsive to the needs and to make an informed decision.  The product, a learning object, responded to the needs of the Natural Sciences Community, but can be reusable by other organizations by making the necessary adjustments as needed.
Innovación educativa en estudios de lectura. Una revisión sistemática en los buscadores Scopus, WoS, Science Direct y Scielo
Authors: Moreno de León Tomás Alfredo , De León Arellano Esperanza
Published: June 2023

El concepto de innovación educativa en estudios de lectura no está completamente definido. En este sentido, es necesario crear consenso sobre lo que se entiende como una innovación educativa en estudios de lectura. Con base en ello, el objetivo del presente trabajo es clasificar los estudios sobre lectura que se autodenominan como innovaciones. Se realizó una revisión sistemática. Para ello se consideró que las investigaciones fuesen sobre innovación educativa respecto a lectura y que hayan sido llevadas a cabo en Iberoamérica. Se utilizaron las palabras de búsqueda “innovación educativa” y “lectura”. Tomando en cuenta estos hallazgos es evidente que la principal línea de producción en la línea de los estudios de lectura es la didáctica de la lectura. En menor medida se ha producido sobre aspectos teóricos o pedagógicos. En contraparte, la producción en innovación educativa de la lectura en términos de lo curricular y lo institucional es casi nula. Se concluye el artículo sintetizando lo que se entiende como innovación educativa en la producción científica del ámbito de la lectura.
Seroprevalencia de anticuerpos contra SARS-CoV-2 entre adultos de un municipio del conurbano bonaerense, República Argentina
Authors: Consiglio Ezequiel , Freytes Carlos , Facal Leandro , Dus Santos María José , Radgty Caffera Magdalena , Mozgovoj Marina , Robledo Iris Celeste , Pilloff Marcela , Fernandez Souto Adriana , Pedrosa Juan
Published: June 2023

El objetivo de este estudio fue determinar la seroprevalencia de anticuerpos contra SARS-CoV-2. Para ello, entre el 21 de noviembre y el 12 de diciembre del año 2020, se desarrolló un estudio transversal en 1182 muestras de residentes de un municipio bonaerense en la Provincia de Buenos Aires, Argentina. Se midió IgG por ELISA en muestras obtenidas vía digitopunción. La prevalencia general fue del 12,2 % (n = 144; IC95 %: 10,3 – 14,0) con un cociente 1:3,8 entre la incidencia acumulada y la observación en nuestro estudio; en mujeres fue del 13,0 % (IC95 %: 10,2 – 15,7) y en hombres, del 10,5 % (IC95 %: 7,6 – 13,3) (p = 0.21). Las prevalencias en grupos etarios fueron las siguientes: 18 a 30 años, del 17,8 % (IC95 %: 12,0 – 23,6); 31 a 60 años, del 12,2 % (IC95 %: 9,3 – 15,1) y 61 años o más, del 9.9 % (IC95 %: 6.9 - 13.9) (p = 0.02). Las prevalencias según los terciles socioeconómicos (por Necesidades Básicas Insatisfechas) fueron para el Tercil I (mejor tercil): 7,7 % (IC95 %: 4,7 – 10,7); el Tercil II: 12.5 % (IC95 %: 8,7 – 16,4); y el Tercil III: 15,2 % (IC95 %: 11,6 – 18,8) (p = 0.002). También hubo diferencias entre las localidades de dicho municipio y entre variables clínicas autorrefenciadas. Se detectaron 12 muestras positivas para IgM: el 1 % del total y el 8,3 % respecto de los casos IgG-positivos. Los estudios de seroprevalencia resultan útiles y podrían contribuir a modelizaciones matemáticas más ajustadas.
Prescripción de psicofármacos a mujeres en situaciones de violencia: perspectivas de profesionales en servicios de apoyo del área metropolitana de Costa Rica
Authors: Rodríguez Fernández Adriana
Published: June 2023

Introducción: Diversas fuentes confirman la sobrerrepresentación de las mujeres en el consumo de psicofármacos, así como diferencias particulares en los patrones de prescripción a mujeres que experimentan violencia. Se trata de un asunto de salud pública que amerita un análisis interdisciplinario junto con un enfoque de género. Objetivo: Explorar la perspectiva de funcionarias institucionales en torno a la prescripción de psicofármacos a mujeres atendidas por situaciones de violencia. Metodología: El estudio es de tipo exploratorio-descriptivo basado en métodos cualitativos de investigación, se integraron fuentes documentales y se consultó a personas expertas mediante la técnica de entrevista semiestructurada. Resultados: El tratamiento con antidepresivos y ansiolíticos es frecuente en mujeres, cuyas principales vías de abastecimiento son la orden médica en el primer nivel de atención en salud y la automedicación. Dicho consumo se relaciona con las consecuencias de la violencia, y las mujeres desconocen la información sobre el uso adecuado de los psicofármacos. Existen diversos matices sobre el rol de los psicofármacos en la ruta crítica de las mujeres afectadas por la violencia, pero existe consenso en que debería ser sólo una de las intervenciones posibles en salud. Conclusiones: Los hallazgos sugieren que en el abordaje del impacto de la violencia contra las mujeres prevalece un paradigma tradicional por parte del sector, pues su principal respuesta es la intervención farmacológica. La violencia por razones de género debe contemplarse dentro de los determinantes sociales de la salud mental y se deben reconocer los riesgos vinculados a su medicalización.
Problem-Based Learning with Multilevel Representation: A Strategy to Master the Ionic Equilibrium in Solution Concepts
Authors: Hamid Abdul , Hikmah Nur , Sholahuddin Arif
Published: June 2023

Mastery of abstract chemistry concepts requires learning strategies to facilitate students to make mental imagery to the submicroscopic level. This study aims to analyze the differences in students' mastery of the ionic equilibrium in salt solutions concept by applying the Problem-Based Learning (PBL) model with multilevel representation. The study applied a quasi-experimental method with a pretest-posttest non-equivalent control group design. The research samples were 61 students of natural science 11th grade SMAN 6 Banjarmasin, which were determined by random cluster sampling. This test instrument is in the form of reasoned-multiple choice with a Content Validity Ratio (CVR) score of 1 (valid), reliability score of 0.96 (very high), difficulty index is moderate to difficult, distinguishing power is moderate to good, and sensitivity item is a sensitive category. Data were analyzed inferentially using an unpaired ttest. This research found that the PBL model with multilevel representation increased the students’ thinking ability at Higher Order Thinking Skills (HOTS) levels. The student's mastery of the ionic equilibrium in salt solutions concept learned by using the PBL model with multilevel representation was better than by using the PBL model.
Students' Creative Thinking Skills on Reaction Rate Topic through Contextual Teaching and Learning Model
Authors: Sihaloho Mangara , Tungkagi Ika Riyana , Ischak Netty Ino , Purwati Dewi Budi , La Kilo Akram
Published: June 2023

This research aims to analyze the contextual teaching and learning (CTL) model on students' creative thinking skills on the reaction rate topic. This quantitative research used true experimental design of pretest-posttest control. The samples used in this study were from 11th grade at SMA Negeri 1 Pinolosian. The experimental class sample was given treatment of the CTL learning model while the control class used conventional learning, respectively, were 31 and 30 students of 93 populations. Based on a questionnaire, students' creative thinking skills were obtained through observation during the learning process. The data analysis of the test instrument was carried out through a validity test using the product-moment correlation technique (significant level = 0.05), Lilliefors normality test, homogeneity of variance test and ttest. The CTL implementation questionnaire and instrument test data were converted into percentages and categorized. The results showed that the application of the CTL model had a positive effect on students' creative thinking abilities, evidenced by the average score of students in the experimental class before and after treatment increased from 14.55 to 74.84. Otherwise, in the control class, the scores were 30.59 to 33.56. The percentage of students' achievement in creative thinking aspects in the experimental class was good (61%-80%) while the control class was less good (21%-40%). The creative thinking abilities of both classes are very different, which is also reflected in the learning outcomes. The value of tcount (2.240) was greater than ttable (2.042) at the significant level = 0.05, and the hypothesis of H0 was rejected, or H1 was accepted. The application of the CTL learning model positively influenced students' creative thinking skills on the reaction rate material. Students' low originality abilities compared to fluency, flexibility, and elaboration abilities were also discussed.
Project-Based Learning (PjBL) Worksheet on the Production of Whey Kefir Paper Soap
Authors: Islamiati Fitri Nur
Published: June 2023

Students often ask about the practical application of concepts and knowledge gained from their coursework. Practicum worksheets provide an opportunity for students to engage in hands-on learning, identify their strengths, learn from their mistakes, and develop enhanced competence as learners. This study aims to develop an effective and valid worksheet on PjBL in whey kefir paper soap. Research and Development (R&D) is used as a method. Worksheet designs were produced using three formulas depending on production techniques. Based on Indonesia National Standard, Formula 1 (8% whey kefir) demonstrated the best qualities of the paper soap. The expert validation test of the worksheets showed a rcount value of 0.84 (valid). The percentage of worksheet answers was very good (88%). Student worksheet feasibility test results showed a rcount value of 0.84 (feasible). Practicum worksheets are categorized as very suitable for use as a learning media in an organic chemistry course.
A (im)possibilidade de mulheres nos alojamentos: disputas de gênero na maçonaria
Authors: De Souza Fernando Rodrigues
Published: June 2023

A maçonaria possui como uma de suas características ser uma instituição progressista, no entanto, diversas questões da modernidade ainda são vistas internamente como tabus. É o caso da participação feminina na instituição, que historicamente causou diversas proibições, controvérsias e cismas, atitudes contrárias ao progressismo maçônico, mesmo sendo possível localizar a presença de mulheres na maçonaria desde a fase operativa da instituição durante a idade média. O presente artigo pretende analisar através de revisão bibliográfica, como as maçonarias francesa e inglesa, que estão entre as principais obediências do mundo maçônico, lidam com a questão, apresentando tensões, aproximações e divergências, trazendo ao debate novas possibilidades e perspectivas.
Investigating the impact of spike protein mutations on SARS-CoV-2 virulence in benin using network centrality and molecular docking approaches
Authors: Thai Quan Ke , Huynh Phuoc , Nguyen Thi Thuong Huyen , Quan Quoc-Dang
Published: June 2023

The COVID-19 pandemic is ongoing and spreading around the world, which means a continuous increase in the number of infections and death. SARS-CoV-2 constantly rapidly stored mutation in the Spike gene to adapt with the host cell. The Spike gene encoded spike protein directly interacts with hACE2 on the human cell surface. Herein, using the network centrality and molecular docking approaches, we detected key mutations that positively affect spike protein. Based on network centrality, we demonstrate that the A23403G (D614G) mutation in the Spike gene is the center of a network which means this mutation has a positive effect on the virus. In addition, analyzing the interaction of spike protein with hACE2, we highlighted that the mutation appeared in the RBD region by changing the electrostatic energy of the complex. Remarkably, mutations N440K, L452R, T478K, E484K, Q493R, and Q498R increased binding free energy of Spike-hACE2 complex due to the change of the side chain into a positive charge. The Eta, Delta, and Omicron variants existed in one or more of these mutations resulting in higher binding free energy and binding affinity than the Wuhan variant indicating sounder interaction with hACE2. In general, mutations appearing on the spike protein tended to cause the surface to become positively charged in order to interact easily with the negative surface of the hACE2 receptor.
X-RAY ASSESSMENT OF THE EFFICIENCY OF HYBRID STABLE-ELASTIC FIXATION UNSTABLE ANKLE FRACTURES
Authors: Sulyma Vadym , Chuzhak Andrii , Filiak Yuliia , Kuz Uliana , Yuriychuk Lubomyr , Symchych Yurii
Published: June 2023

The level of complications after treatment of patients with bone fractures stays high 22–60 %. Understanding ankle-foot joint biomechanics is critical for choosing trendy methods hybrid fixation or the traditional fixation of the fibula plate and positional screw for distal syndesmosis. Objective. To analyze the effectiveness of surgical treatment of patients with unstable trans- and suprasyndesmosis fractures with hybrid stable-elastic fixation (HSEF) in comparison with the traditional method of fixation with a bone plate with a positioning screw according to X-ray morphometric parameters. Methods. 82 patients with unstable trans- and suprasyndesmotic fractures of the fibula. The main (I group) of the study consisted of 18 (21,95 %) patients with an average age of (41,6 ± 15,5) years who operated with hybrid stable-elastic fixation, and the control (II group) — 64 (78.05 %) patients with an average age of (52,4 ± 15,7) years, who operated with a bone plate with a positioning screw. Results. The TTA was 0.63 times lower than in patients after traditional fixation. For type C fractures in patients after HSEF, the value of TTA was also 0.78 times (p = 0.0005). Conclusions. The analysis of X-ray morphometric parameters of the angle of inclination of the talus bone and the talus-tibia angle confirms the advantage of hybrid stable-elastic fixation in trans- or supra-syndesmotic fractures of the fibula with damage to the distal inter-tibial syndesmosis. According to the value of the index of the angle of inclination of the talus in patients after hybrid stable-elastic fixation, lower risks of ankle-foot joint instability in the p/o period were found than in patients after traditional osteosynthesis. Median values of the talus-tibia angle indicated a tendency to shorten the fibula in patients after traditional osteosynthesis.
CHANGES IN MARKERS OF BONE TISSUE REMODELING AND THE INFLAMMATORY PROCESS IN THE BLOOD SERUM OF WHITE RATS IN CASE OF DEFECT FILLING OF THE FEMUR WITH IMPLANTS BASED ON POLYLACTIDE AND TRICALCIUMPHOSPHATE WITH MESENCHYMAL STEM CELLS
Authors: Gontar Nazar
Published: June 2023

Objective. Based on the analysis of markers of inflammation and metabolism of bone tissue in the blood serum of laboratory rats, to evaluate the course of bone remodeling after filling the defect in the distal metaphysis of the femur with 3D-printed implants based on polylactide and tricalcium phosphate (3D-I) alone or in combination with mesenchymal stromal cells (MSCs). Methods. 53 white rats were used, which were divided into groups: intact (5 animals) — the operation was not performed; Control (15) — 3D-I; Experiment I (15) — 3D-I + cultured alloMSCs; Experiment II (15) — 3D-I + introduction of alloMSCs into the area of surgical intervention 7 days after implantation. The following were studied: the content of glycoproteins (GP), interleukin-6 (IL-6), osteocalcin, chondroitin sulfates (CS), total protein, calcium, alkaline (AlP) and acid phosphatase (AP) activity, and their ratio, mineralization indices were calculated. Results. Compared with intact animals, higher indicators were determined in the rats of the Control group: the content of GP by 39.73; 32.88; 23.29 %; CS — 250.00; 222.09 and 196.51 %, AlP activity — 81.67, 51.03, 39.36 %, on the 15th, 30th, and 90th days of the experiment; IL-6 — 44.89; 60.06 % on the 15th and 30th days. In the rats of the Experiment I g roup: the content of GP — by 82.19; 65.75, 57.53 %, IL-6 — 72.14; 96.59; 79.88 %, CS — 306.98; 276.74; 253.49 %; AlP activity — 63.73; 129.70; 51.28 %, on the 15th, 30th and 90th days of the experiment. In the Experiment II group: on the 15th, 30th and 90th days, the content of GP was higher by 27.40; 26.03; 129.18 %; CS — by 175.58; 137.21 and 115.12 %; AlP activity — 192.99; 178.02, 76.31 %; on the 15th and 30th days: IL-6 — by 37.46; 20.74 %. Conclusions. In the case of filling the defect with 3D-printed implants, biochemical signs of moderate inflammation were determined; 3D-printed implants together with MSCs — pronounced inflammation, slowing of bone formation, formation of connective tissue; 3D-printed implants with postoperative injection of MSCs — moderate inflammation and optimal conditions for healing the defect with bone tissue.
CHONDROSARCOMA IN THE XXI CENTURY
Authors: Vyrva Oleg
Published: June 2023

Chondrosarcoma (CHS) is a rare oncopathology, is the third most common primary bone tumor after multiple myeloma and osteosarcoma. It accounts for about 25 % of the total number of bone sarcomas. CHS mainly affects adults and occurs more often in people older than 40 years, in children and adolescents it is less than 5 % of all cases of primary CHS. The most common CHS sites can be any bone containing cartilage, but most often this tumor is found in pelvis, femur and shoulder bones, and ribs. CHS are divided: by origin (primary and secondary), anatomical site (central — inside the bone marrow canal, peripheral — inside the existing osteochondroma, periosteal — on the bone surface), histological degrees GI-GII-GIII. The WHO classification (2020) includes central normal, secondary peripheral, periosteal, dedifferentiated, mesenchymal and clear-cell CHS. More than 90 % of conventional CHS are tumors of low and medium malignancy degree, with a low potential for metastasis. CHS is caused by mutations in genes that control bone growth and development. The main risk factors are the patientʼs age, previous radiation, genetic factors and predisposition to oncological diseases. Diagnosis of CHS is based on a complex algorithm, which involves collecting the patient's complaints, anamnesis, clarifying clinical symptoms, imaging (X-ray, CT, MRI), histopathological picture. The biopsy conclusion is the most important in establishing the final diagnosis. However, there are several tumors whose histological picture is similar to CHS: enchondroma, chondroblastoma, osteosarcoma, giant cell tumor of bone, dedifferentiated liposarcoma, synovial sarcoma. The CHS treatment protocol is determined based on the results of anamnestic data, imaging, histopathological results, CHS classification, and its final tumor subtype. The «gold standard» remains surgical removal of the tumor. Radiation and chemotherapy don’t play a significant role in the treatment of CHS, but require further study. Targeted and immunotherapy have a certain potential, even with a high degree of CHS resistance to traditional chemotherapy.
The floristic composition, morphometric and the adaptation of a new species: Malva subovata in the matorral of Algeria (Djbel fellaoucen)
Authors: GHALEM Sarra , HASSANI Faiçal , ABDELLI Imane , BELHADJI Amina , BEKKAL BRIKCI Sohayb
Published: June 2023

This study is based on a ruderal species: Malva subovata = Lavatera maritima which should normally grow in the littoral, under maritime influence. Its presence in the matorrals is highlighted for the first time. as it has been found far from its natural habitat. The comparison of the different biological and morphological spectra shows the importance of the therophytes that confirms the phenomenon of degradation. This degradation shows a depletion of the floristic procession, especially concerning sylvatic species which have given way to ephemeral therophytes and chamæphytes. Despite the presence of a shrubby layer, it is no longer a question of a forest ecosystem but of a pre-forest ecosystem. To better understand the characteristics of this Malvaceae in terms of resistance and adaptation in matorrals, we often use the phytomass of all anthropozoic formations and more specifically the phytomass of the species that characterize this group.  In our case, this is Malva subovata. The results of the morphometric study constitute a basic element for the evaluation of the adaptive potential of our Lavatère against the degradation factors.
Phytoremediation potential of native plants: Biomonitoring approach in contaminated soils
Authors: AYDI Samir , Sameh SASSI AYDI  , BEN SALAH Ghada , DEKHIL Amani , Rami RAHMANI  , BOUAJILA Abdelhakim , MOHAMED Niveen A. , ABDELLY Chedly
Published: June 2023

Phytoremediation has been used to remediate contaminated site. This study aims to investigate the potential of the selected native plants to accumulate and tolerate chemical pollutants. Two sites were selected in Southern Tunisia lands (Gabès), i) Gannouch located near the chemical industrial complex and ii) Zerkineis around 35 kilometres far from the industrial activities. Soil and plants were sampled and analysed for cadmium (Cd), lead (Pb), copper (Cu), zinc (Zn) and fluorine (F) concentrations. The native plants were Bassia indica and Chenopodium album, with no obvious morphological toxic symptoms. Results indicated that the upper soil layers were alkaline and salt affected with low organic matter content. The highest levels of fluoride and heavy metal were found in Ghannouch. The contents of Cd, Cu, Pb, Zn and F in the roots, stem and leaves of the native plants exceed the normal ranges and the phytotoxic level. Bioconcentration factor (BCF), translocation factor (TF), and biological accumulation factor (BAF) were determined for each element. Based on these factors the selected plants were classified as excluders, indicators, and accumulators for each element. The native plants, studied herein, seems to have significant potential for phytoremediation, thus they may be used as biomonitors in contaminated soils. Bassia indica identified as Cd, Cu and Pb accumulator, could be an excellent candidate for phytoextraction and phyto stabilization because of its F excluder performance.
Conditions for making plant dispersions based on nature-like technologies
Authors: LEONOVA Svetlana , VEBER Anna , KALUGINA Olesya , BADAMSHINA Elena , ZHIARNO Malgorzhat
Published: June 2023

An objective of this study was to examine the possible use of sprouted grains of domestically selected legumes to produce dairy alternative products. The paper presents the results of a comprehensive assessment of the food properties of ‘Chishminskii 95’, ‘Chishminskii 229’, ‘Pamiati Hangildina’, ‘Iuldash’ pea varieties and ‘Nerussa’, ‘Lukeria’, ‘Omichka’ bean varieties of Russian selection. The work investigated the water absorption kinetics under different temperature conditions and established germination patterns for a model medium (drinking water treated with an electromagnetic field) being as follows: germination temperature is 18 ± 2 °C; the soaking period lasts from 5 to 8 hours (depending on the variety); the germination time from 0.54 - 0.62 to 0.70 - 0.81 days (depending on the variety). The work analyses their microstructure and changes in the germination process. The proteolytic activity of bean trypsin before and after germination has been proved to be lower than that of peas. At the same time, the proteolytic activity of trypsin after pea and bean grain germination increased in all samples. The grain digestibility as a result of germination has increased; the ratio of essential and non-essential amino acids has changed in favor of the former; the fractional composition of protein has changed to higher content of albumin and globulin and lower level of glutelin. The results of this study indicate that the most suitable varieties to produce vegetable milk are ‘Omichka’, ‘Lukeria’ beans and ‘Pamiati Hangildina’ peas.
Variation in the susceptibility of blackberry varieties to Drosophila suzukii infestation
Authors: HULUJAN Ionuţ B. , BUNESCU Horia , FLORIAN Vasile , OLTEAN Ion , GORGAN Monica , POPUȚA Sorina D. , FLORIAN Teodora
Published: June 2023

Drosophila suzukii Matsumura (Diptera: Drosophilidae) is an invasive polyphagous species, that is frequently found in blackberry culture, affecting the commercial value of fruits. Between 2020-2021, in the blackberry collection of the University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca (Romania), were carried out observations on susceptibility of five cultivated varieties (‘Thornless’, ‘Loch Ness’, ‘Thornfree’, ‘Thornless Evergreen’ and ‘Navaho’) to D. suzukii. In 2020, the attack frequency in the whole collection was 78.2%. ‘Thornfree’ had the highest attack frequency, 89% while ‘Loch Ness’ was the least susceptible with a frequency of attack of 71%. In 2021, the overall proportion of damaged fruits in the collection was 59.6%. The most susceptible was ‘Thornless’ with an attack frequency of 66% and ‘Thornless Evergreen’ with only 49%. The incidence of infestation by the spotted-wing Drosophila suzukii is influenced by the thermal characteristics of the year, with temperatures higher than 30 °C impacting the fertility of females and the viability of eggs. In the year 2020, the average number of larvae per fruit collected was 6.4, while the average number of larvae in the ‘Loch Ness’ cultivar was 9.4, with a maximum of 48 larvae per fruit. In 2021, an average of 3.4 larvae per fruit were observed, with variations ranging from 2.8 larvae in the ‘Thornfree’ cultivar to 4.2 larvae in the ‘Navaho’ cultivar.
In vitro direct organogenesis of the medicinal single-mountain local prioritized vulnerable Greek endemic Achillea occulta under different medium variants
Authors: SARROPOULOU Virginia , MALOUPA Eleni , GRIGORIADOU Katerina
Published: June 2023

Achillea species are of high medicinal value based on their use in traditional medicine and their content in secondary metabolites with potential application in dermatology and cosmetic industry. Achillea occulta is a single-mountain local prioritized vulnerable Greek endemic encountering propagation problems through seeds and root division. In vitro propagation can be used for the rapid and mass production of clonal plants complying with phytochemical and sanitary quality criteria. In this study, several culture medium variants were tested including the effect of different indole-3-butyric acid (IBA) concentrations with 6-benzyladenine (BA), different cytokinin types, agar concentrations, and zeatin (ZT) sterilization methods on in vitro shoot proliferation, as well as the effect of different auxin types and concentrations on in vitro rooting and ex vitro survival rate of shoot-tip explants. The results showed BA was the most preferable cytokinin type as evidenced by all proliferation attributes and autoclaved ZT provided longer shoots. Therefore, the two more promising and cost-effective treatments for proliferation were 2.2 μM BA + 0.25 μM IBA or 4.4 μM BA + 0.5 μM IBA after 21 days of culture on modified Murashige-Skoog (MS) medium (x2 Fe) enriched with 20 g L-1 sucrose and 6-7 g L-1 Agar (90-93.8% shoot formation, 2.7-2.9 shoots 0.4-0.6 cm long, 2.5-2.6 proliferation rates). Explants rooted better after 21 days of culture on plain MS medium with 2 μM IBA or 1 μM α-naphthalene acetic acid (NAA) (13.3-20% rooting, 1-1.5 roots 1.8-2 cm long). NAA gave more roots, IBA longer roots, while both auxins exhibited highest rooting (13.33-20%) and ex vitro survival rates (50-66.7%).
Cloning and analysis of DlERF23 gene in flower induction
Authors: SANG Xuelian , REN Ci , JUE Dengwei
Published: June 2023

Irregular flowering is a serious problem in longan production. Identifying the flower induction-related genes and analyzing their regulation mechanism is the key to solve this problem. The APETALA2/ethylene responsive factor (AP2/ERF) superfamily members are transcription factors (TFs) that regulate diverse developmental processes, including flowering time, and stress responses in plants. However, there is still no research about AP2/ERF involved in the regulation of longan flower induction. In the present study, a AP2/ERF TF member DlERF23 was cloned from longan (Dimocarpus longan). It has a typical AP2 domain with the coding sequence (CDS) of DlERF23 is 552 bp in length and encodes 184 amino acids. The molecular weight of DlERF23 protein was 20.41 kda and the theoretical isoelectric point (PI) was 7.69. The amino acid sequence of DlERF23 protein had the highest similarity with CsERF23 (XP_006478313.1) of Citrus sinensis and CcERF23 (XP_006441807.2) of Citrus clementina. The results of qRT-PCR showed that the relative expression level of DlERF23 gene in pericarp was higher, followed by stem, leave, flower and flower bud. Meanwhile, DlERF23 gene significant down-regulated in the early stage of flower induction in ‘Sijimi’ (SJ) longan and up-regulated in the late stage of flower induction in ‘Shixia’ (SX). The results of transient expression of Arabidopsis protoplasts showed that the fluorescence signal was mainly concentrated in the nucleus. Moreover, overexpression of DlERF23 in Arabidopsis promoted early flowering. These results provide useful information for revealing the biological roles of DlERF23 in longan and increase our understanding of the AP2/ERF superfamily members in fruit trees.
Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+
Authors: BOKRI Hana , KAHLAOUI Samiha , HCINI Kheiria , DHOUEIBI Manel , HARZALLAH-SKHIRI Fethia , STAMBOULI-ESSASSI Sondes
Published: June 2023

The present work describes an efficient micropropagation and callus induction protocol of Limbarda crithmoïdes L. (Asteraceae), a halophyte species with medicinal and horticultural interests. The objective was to identify the culture media that support the best organogenesis and callogenic expressions of four types of aerial explants; nodal and internodal segments and leaf portions, by varying the mineral composition of twenty culture media by adding increasing concentrations of Na+ (from 0-100 mM) and K+ (from 0-50 mM). After two months of culture, parameters relating to the various expressions of organogenesis and callogenesis were measured. Results showed that the K+ and Na+ interactions affect the explants development. The combination of high concentrations of Na+ (50, 100 mM) and K+ (50 mM) ions allowed the most important regeneration of the axillary shoots (70.6-100%), root neoformation (82.6-96.1%) and callus induction (76.3-100%). Rooted plantlets with well-developed axillary shoots have been successfully acclimatized with a 93% of survival rate. The selected media would allow a large-scale multiplication of this medicinal species, without adding exogenous phytohormones, and could be used for the micropropagation of other threatened halophyte species or for the production of callus; rich in secondary metabolites.
Deciphering the myth of cold tolerance in soybean: An overview of molecular breeding applications
Authors: JIAN Wei , QIANG Qiu , Guan YIJUN  , Ran QIUYAN  , JIANING Guan , QIAN Zhao , CHUNGANG Zhao , RASHEED Adnan , Wang YUEQIANG  , CHEN Liang , Nazer MANZOOR  , QARI Sameer H. , BARQAWI Aminah A. , WAHAB Hafiz A. , ZHIMING Xie , Wang XIAOXUE
Published: June 2023

The soybean is a source of several dietary components, including milk, protein, and oil. Cold stress has significantly curtailed soybean growth and yield in large areas and caused a high risk to global food security.  The main objective of soybean breeders is to improve soybean resistance to cold stress. Conventional breeding approaches have made significant progress in developing cold tolerance in soybean; however, the high cost and complex genetic mechanism of cold tolerance hindered the large scale of these techniques. Molecular tools like quantitative trait loci (QTL), genome-wide association studies (GWAS), transcription factors (TFs), genetic engineering, and transcriptome have been used to identify cold tolerant genes/QTL and to develop soybean cultivars tolerant to cold stress. Clustered, regularly interspaced short palindromic repeats (CRISPR/Cas9) is used to increase the abiotic stress tolerance in soybean; however, its use to edit the cold tolerance genes in soybean is limited. Mapping of QTL has accelerated the master-assisted selection (MAS) in soybean. This review presents a detailed overview of molecular techniques and their use in developing cold-tolerant soybean cultivars. Using CRISPR/Cas9 would increase the speed of molecular breeding for cold tolerance in soybean. This information will assist soybean researchers in uncovering the basis of cold stress tolerance in soybean and adopting the most suitable way to breed the cold tolerant cultivars which can thrive under the extreme pressure of cold stress.
Effect of postharvest exogenous edible coating treatments on inhibitor browning and maintaining quality of fresh mushroom
Authors: ABOU-ELWAFA Saleh M. , HAFFEZ Mona M. , AHMED Atef F. , ABOU EL-YAZIED Ahmed , ABDELAAL Khaled
Published: June 2023

The shelf-life of fresh mushrooms is limited to 1-3 days at ambient conditions and antioxidant characters are reduced and acquire a brown colour during post-harvest storage. Therefore, the present study was conducted to investigate the effect of some postharvest exogenous edible coating treatments of immersed in six different solutions for 5 min of calcium chloride (2%, 4%), chitosan (1%, 2%), CMC (1%, 2%) and control (distilled water) on reducing the browning, microbial load and maintaining quality during storage for 15 days at 4 ± 1 °C and 95% RH, during two successive seasons of 2021 and 2022 in 4th and 6th of January in the first and second seasons respectively. The obtained results revealed that dipping mushroom plants in a solution of chitosan 2% or calcium chloride 4% was the best treatment for reducing weight loss, while chitosan 2% for 5 min was the most effective treatment in reducing browning, PPO activity, total phenolic, flavonoids contents and inhibiting microbial counts for 12 days at 4 ± 1 °C and 95% RH.
Efficient utilization of pine logs in northern Mexico through training
Authors: RASCÓN-SOLANO Joel , AGUIRRE-CALDERÓN Oscar A. , NÁJERA-LUNA Juan A. , ALANÍS-RODRÍGUEZ Eduardo , JIMÉNEZ-PÉREZ Javier , TREVIÑO-GARZA Eduardo J.
Published: June 2023

The timber industry and other sectors that rely on forest management, would benefit from a deeper understanding of the extraction work carried out by properly trained workers. The objective of this research was to evaluate the effect of training operational personnel on the efficient utilization of harvested pine logs in the southern region of the state of Chihuahua, in northern Mexico. Two samplings of the logging present in the cutting area of the ejido Basihuare (communal land) were carried out, taking into consideration the diameter with and without bark, visual quality, length, and use for reinforcement. The sampling involved recording 116 logs prior to a directional felling and log dimensioning training process, and 336 logs divided into three sampling blocks after the training. The data was analysed with tests for variance homogeneity, and ANOVA analyses were performed at a significance level of 0.05. It was found that training forestry harvest personnel allows for an improvement in the process of classifying logs destined for industrial use according to their dimension and visual quality. Additionally, the training allowed for the proper adjustment of reinforcements applied in the log dimensioning process, and as a result, it was possible to efficiently utilize available resources, significantly reducing the generation of waste after resizing of the wood products. For the advancement of the forestry sector, it is necessary to prioritize training as a driving element of quality to raise operational efficiency parameters of production and, consequently, promote the evolution of the production process.