bluuebunny/crossref_metadata_2025 huggingface dataset:
Contact-Patch-Size Distribution and Limits of Self-Affinity in Contacts between Randomly Rough Surfaces
Authors: Müser Martin H. , Wang Anle
Published: September 2018

True contact between solids with randomly rough surfaces tends to occur at a large number of microscopic contact patches. Thus far, two scaling regimes have been identified for the number density n ( A ) of contact-patch sizes A in elastic, non-adhesive, self-affine contacts. At small A, n ( A ) is approximately constant, while n ( A ) decreases as a power law at large A. Using Green’s function molecular dynamics, we identify a characteristic (maximum) contact area A c above which a superexponential decay of n ( A ) becomes apparent if the contact pressure is below the pressure p cp at which contact percolates. We also find that A c increases with load relatively slowly far away from contact percolation. Results for A c can be estimated from the stress autocorrelation function G σ σ ( r ) with the following argument: the radius of characteristic contact patches, r c , cannot be so large that G σ σ ( r c ) is much less than p cp 2 . Our findings provide a possible mechanism for the breakdown of the proportionality between friction and wear with load at large contact pressures and/or for surfaces with a large roll-off wavelength.
CdS/CdTe Heterostructures for Applications in Ultra-Thin Solar Cells
Authors: Gutierrez Z-B Karla , G. Zayas-Bazán Patricia , De Melo Osvaldo , De Moure-Flores Francisco , Andraca-Adame José A. , Moreno-Ruiz Luis. A. , Martínez-Gutiérrez Hugo , Gallardo Salvador , Sastré-Hernández Jorge , Contreras-Puente Gerardo
Published: September 2018

The preparation of ultra-thin semi-transparent solar cells with potential applications in windows or transparent roofs entails several challenges due to the very small thickness of the layers involved. In particular, problems related to undesired inter-diffusion or inhomogeneities originated by incomplete coverage of the growing surfaces must be prevented. In this paper, undoped SnO2, CdS, and CdTe thin films with thickness suitable for use in ultra-thin solar cells were deposited with a radiofrequency (RF) magnetron sputtering technique onto conductive glass. Preparation conditions were found for depositing the individual layers with good surface coverage, absence of pin holes and with a relatively small growth rate adapted for the control of very small thickness. After a careful growth calibration procedure, heterostructured solar cells devices were fabricated. The influence of an additional undoped SnO2 buffer layer deposited between the conductive glass and the CdS window was studied. The incorporation of this layer led to an enhancement of both short circuit current and open circuit voltage (by 19 and 32%, respectively) without appreciable changes of other parameters. After the analysis of the cell parameters extracted from the current-voltage (I-V) curves, possible origins of these effects were found to be: Passivation effects of the SnO2/CdS interface, blocking of impurities diffusion or improvement of the band alignment.
Characterisation of InGaN by Photoconductive Atomic Force Microscopy
Authors: Weatherley Thomas F. K. , Massabuau Fabien C.-P. , Kappers Menno J. , Oliver Rachel A.
Published: September 2018

Nanoscale structure has a large effect on the optoelectronic properties of InGaN, a material vital for energy saving technologies such as light emitting diodes. Photoconductive atomic force microscopy (PC-AFM) provides a new way to investigate this effect. In this study, PC-AFM was used to characterise four thick (∼130 nm) In x Ga 1 − x N films with x = 5%, 9%, 12%, and 15%. Lower photocurrent was observed on elevated ridges around defects (such as V-pits) in the films with x ≤ 12 %. Current-voltage curve analysis using the PC-AFM setup showed that this was due to a higher turn-on voltage on these ridges compared to surrounding material. To further understand this phenomenon, V-pit cross sections from the 9% and 15% films were characterised using transmission electron microscopy in combination with energy dispersive X-ray spectroscopy. This identified a subsurface indium-deficient region surrounding the V-pit in the lower indium content film, which was not present in the 15% sample. Although this cannot directly explain the impact of ridges on turn-on voltage, it is likely to be related. Overall, the data presented here demonstrate the potential of PC-AFM in the field of III-nitride semiconductors.
Oxidation Behavior of Multilayer Hard Coatings (TiCN/Al2O3/TiN) in Process of Recycling Coated Multicomponent Hardmetal Scrap
Authors: Kuang Hai , Tan Dunqiang , He Wen , Yi Zhiqiang , Zou Zhihang , Wang Xiaoru
Published: September 2018

The coating is one of the biggest problems in the recycling of coated multicomponent hardmetal scraps. The isothermal oxidation behavior of WC-Co multicomponent cemented carbide inserts with a TiCN/Al2O3/TiN hard coating in the recycling process was investigated. The oxidation rate slowed down as the protective coating blocked element diffusion. A rapid oxidation rate was obtained when they were milled into powders and isothermally oxidized at 900 °C. A rapid path for element diffusion was provided by the defects, which were promoted by stress, expansion, and gas volatilization. Both the TiN and TiCN layers were oxidized to a porous TiO2 scale, while the Al2O3 phase remained and the dense Al2O3 layer acted as a barrier for its good oxidation resistance. Pieces of the Al2O3 layer were obviously seen in the final oxides. This provides critical information to reduce the negative effect of coatings and improve the performance of recycled WC powders and hard alloys.
Two Novel Aspochalasins from the Gut Fungus Aspergillus sp. Z4
Authors: Li Xinyang , Ding Wanjing , Wang Pinmei , Xu Jinzhong
Published: September 2018

Two novel aspochalasins, tricochalasin A (1) and aspochalasin A2 (2), along with three known compounds (3–5) have been isolated from the different culture broth of Aspergillus sp., which was found in the gut of a marine isopod Ligia oceanica. Compound 1 contains a rare 5/6/6 tricyclic ring fused with the aspochalasin skeleton. The structures were determined on the basis of electrospray ionisation mass spectroscopy (ESIMS), nuclear magnetic resonance (NMR) spectral data, and the absolute configurations were further confirmed by modified Mosher’s method. Cytotoxicity against the prostate cancer PC3 cell line were assayed by the MTT method. Compound 3 showed strong activity while the remaining compounds showed weak activity.
The Pre-Transplant Drop in Panel-Reactive Antibodies Titer Evaluated Using Complement-Dependent Cytotoxicity (PRA-CDC) and the Risk of Early Acute Rejection in Sensitized Kidney Transplant Recipients
Authors: Kolonko Aureliusz , Bzoma Beata , Giza Piotr , Styrc Beata , Sobolewski Michał , Chudek Jerzy , Dębska-Ślizień Alicja , Więcek Andrzej
Published: September 2018

Background: The panel-reactive antibodies that use the complement-dependent cytotoxicity test (PRA-CDC) are still a standard method for monitoring the degree of immunization in kidney transplant candidates on active waiting lists in some countries, including Poland. The aim of this study was to analyze the relationship between the maximum and the last pre-transplant PRA titer on the percentage of positive cross-matches and rate of early acute rejection episodes. Material and methods: The retrospective analysis included 528 patients from two transplant centers. All patients were divided into three groups, depending on their peak and last pre-transplant PRA titers. There were 437 (82.8%) patients with peak PRA <20% (non-sensitized group, non-ST) and 91 (17.2%) patients with peak PRA >20%. Among the latter group, 38 had maintained PRA level >20% at the time of transplantation (sensitized patients, ST), whereas 53 had pre-transplant PRA ≤20% (previously sensitized patients, prev-ST). Results: The percentages of positive crossmatches were 76.9% in ST and 53.7% in prev-ST groups versus 18.4 in non-ST group (both p < 0.001). The acute rejection rates were 18.9, 17.6 and 6.8%, respectively (p < 0.001 for ST or prev-ST versus non-ST). The pre-transplant PRA titer drop did not decrease the risk of early acute rejection [OR = 1.09 (95% CI: 0.31–3.85)] in a multiple logistic regression analysis. The occurrences of primary graft non-function and delayed graft function were similar in all study groups. Conclusions: Previously immunized kidney transplant candidates even with substantial decrease in pre-transplant PRA-CDC levels are still at high immunological risk when compared with non-immunized patients, and they should receive lymphocyte-depleting induction therapy.
Thin Film Composite Membrane for Oily Waste Water Treatment: Recent Advances and Challenges
Authors: Ahmad Nor Akalili , Goh Pei Sean , Abdul Karim Zulhairun , Ismail Ahmad Fauzi
Published: September 2018

Oily wastewater discharge from various industry processes and activities have caused dramatic impacts on the human and environment. Treatment of oily wastewater using membrane technology has gained worldwide attention due to its efficiency in removing the amount and concentration of oil and grease as well as other specific pollutants in order to be reused or to fulfill stringent discharge standard. The application of thin film composite (TFC) membrane in reverse osmosis (RO) and forward osmosis (FO) for oily wastewater treatment is an emerging and exciting alternative in this field. This review presents the recent and distinctive development of TFC membranes to address the issues related to oily wastewater treatment. The recent advances in terms of TFC membrane design and separation performance evaluation are reviewed. This article aims to provide useful information and strategies, in both scientific knowledge advancement and practical implementation point of view, for the application TFC membrane for oily wastewater treatment.
Effects of B2O3 on Viscosity, Structure, and Crystallization of Mold Fluxes for Casting Rare Earth Alloyed Steels
Authors: Cai Zeyun , Song Bo , Li Longfei , Liu Zhen , Cui Xiaokang
Published: September 2018

Amounts of rare earth oxides were transferred into mold fluxes for the continuous casting of rare earth alloyed heavy railway steels. B2O3 was added to improve the viscosity properties and the crystallization behavior of this traditional mold flux. Thus, in this paper, effects of B2O3 on viscosity, structure, and crystallization of CeO2-bearing mold fluxes for casting rare earth alloyed heavy railway steels were investigated by rotating cylinder method, Raman spectroscopy, and magic angle spinning nuclear magnetic resonance (MAS NMR). Experimental results revealed that with the addition of B2O3, viscosity values of mold fluxes at some certain temperatures decreased significantly. By analyzing the structure of glassy mold fluxes, it can be observed that B2O3 behaved as a network-forming oxide within the studied system. However, the introduction of the weak bond energy of B–O and the transition from four-fold coordination boron to three-fold coordination boron with loose structural units mainly reduced the viscosity. Moreover, results of X-ray diffraction (XRD) and differential scanning calorimetry (DSC) confirmed that B2O3 decreased the crystallization of the crystal phase Na4Ca4(Si6O18) and the solidus temperature of mold fluxes. Thus, these results obtained can provide guidelines for designing new mold fluxes for casting rare earth alloyed steels.
Development of High-Performance SiCp/Al-Si Composites by Equal Channel Angular Pressing
Authors: Xu Qiong , Ma Aibin , Wang Junjie , Sun Jiapeng , Jiang Jinghua , Li Yuhua , Ni Chaoying
Published: September 2018

Relatively low compactness and unsatisfactory uniformity of reinforced particles severely restrict the performance and widespread industry applications of the powder metallurgy (PM) metal matrix composites (MMCs). Here, we developed a combined processing route of PM and equal channel angular pressing (ECAP) to enhance the mechanical properties and wear resistance of the SiCp/Al-Si composite. The results indicate that ECAP significantly refined the matrix grains, eliminated pores and promoted the uniformity of the reinforcement particles. After 8p-ECAP, the SiCp/Al-Si composite consisted of ultrafine Al matrix grains (600 nm) modified by uniformly-dispersed Si and SiCp particles, and the composite relative density approached 100%. The hardness and wear resistance of the 8p-ECAP SiCp/Al-Si composite were markedly improved compared to the PM composite. More ECAP passes continued a trend of improvement for the wear resistance and hardness. Moreover, while abrasion and delamination dominated the wear of PM composites, less severe adhesive wear and fatigue mechanisms played more important roles in the wear of PM-ECAP composites. This study demonstrates a new approach to designing wear-resistant Al-MMCs and is readily applicable to other Al-MMCs.
Evolution Properties of Tribological Parameters for Steel Wire Rope under Sliding Contact Conditions
Authors: Chang Xiang-Dong , Peng Yu-Xing , Zhu Zhen-Cai , Zou Sheng-Yong , Gong Xian-Sheng , Xu Chun-Ming
Published: September 2018

Friction and wear seriously affect the safety use of winding hoist wire rope and the damage is a gradual process in practical application. In this paper, the tribological properties of a wire rope under different sliding distances were investigated. The evolutions of the coefficient of friction (COF), temperature rise, and wear characteristics under different contact loads and strokes were analyzed by a series of experiments. The results show that fluctuation of the friction is large in the early stages (before 10 min) and the major peaks depend on the stroke. When the contact load is 150 N, the COF increases most rapidly. It grows from approximately 0.48 to approximately 0.61 with the sliding distance. Additionally, the temperature rise in the wear region is higher under the large stroke (30 mm) and contact load (150 N). The maximum temperature rises are approximately 7.5 °C and 7.1 °C, respectively. Furthermore, it is approximately after 7200 mm that the temperature rise reaches a relatively stable stage. The wear scar region increases with the sliding distance and the maximum wear loss is approximately 65.5 mg at a load of 150 N. The major wear mechanisms of the wire rope are fatigue and adhesion wear.
Lung Protection Strategies during Cardiopulmonary Bypass Affect the Composition of Bronchoalveolar Fluid and Lung Tissue in Cardiac Surgery Patients
Authors: Maltesen Raluca G. , Buggeskov Katrine B. , Andersen Claus B. , Plovsing Ronni , Wimmer Reinhard , Ravn Hanne B. , Rasmussen Bodil S.
Published: September 2018

Pulmonary dysfunction is among the most frequent complications to cardiac surgeries. Exposure of blood to the cardiopulmonary bypass (CPB) circuit with subsequent lung ischemia-reperfusion leads to the production of inflammatory mediators and increases in microvascular permeability. The study aimed to elucidate histological, cellular, and metabolite changes following two lung protective regimens during CPB with Histidine-Tryptophan-Ketoglutarate (HTK) enriched or warm oxygenated blood pulmonary perfusion compared to standard regimen with no pulmonary perfusion. A total of 90 patients undergoing CPB were randomized to receiving HTK, oxygenated blood or standard regimen. Of these, bronchoalveolar lavage fluid (BALF) and lung tissue biopsies were obtained before and after CPB from 47 and 25 patients, respectively. Histopathological scores, BALF cell counts and metabolite screening were assessed. Multivariate and univariate analyses were performed. Profound histological, cellular, and metabolic changes were identified in all patients after CPB. Histological and cellular changes were similar in the three groups; however, some metabolite profiles were different in the HTK patients. While all patients presented an increase in inflammatory cells, metabolic acidosis, protease activity and oxidative stress, HTK patients seemed to be protected against severe acidosis, excessive fatty acid oxidation, and inflammation during ischemia-reperfusion. Additional studies are needed to confirm these findings.
A Novel, Hybrid-Integrated, High-Precision, Vacuum Microelectronic Accelerometer with Nano-Field Emission Tips
Authors: Liu Haitao , Wei Kai , Li Zhengzhou , Huang Wengang , Xu Yi , Cui Wei
Published: September 2018

In this paper, a novel, hybrid-integrated, high-precision, vacuum microelectronic accelerometer is put forward, based on the theory of field emission; the accelerometer consists of a sensitive structure and an ASIC interface (application-specific integrated circuit). The sensitive structure has a cathode cone tip array, a folded beam, an emitter electrode, and a feedback electrode. The sensor is fabricated on a double-sided polished (1 0 0) N-type silicon wafer; the tip array of the cathode is shaped by wet etching with HNA (HNO3, HF, and CH3COOH) and metalized by TiW/Au thin film. The structure of the sensor is finally released by the ICP (inductively coupled plasma) process. The ASIC interface was designed and fabricated based on the P-JFET (Positive-Junction Field Effect Transistor) high-voltage bipolar process. The accelerometer was tested through a static field rollover test, and the test results show that the hybrid-integrated vacuum microelectronic accelerometer has good performance, with a sensitivity of 3.081 V/g, the non-linearity is 0.84% in the measuring range of −1 g~1 g, the average noise spectrum density value is 36.7 μV/ Hz in the frequency range of 0–200 Hz, the resolution of the vacuum microelectronic accelerometer can reach 1.1 × 10−5 g, and the zero stability reaches 0.18 mg in 24 h.
Support Vector Regression Approach to Predict the Design Space for the Extraction Process of Pueraria lobata
Authors: Wang Yaqi , Yang Yuanzhen , Jiao Jiaojiao , Wu Zhenfeng , Yang Ming
Published: September 2018

A support vector regression (SVR) method was introduced to improve the robustness and predictability of the design space in the implementation of quality by design (QbD), taking the extraction process of Pueraria lobata as a case study. In this paper, extraction time, number of extraction cycles, and liquid–solid ratio were identified as critical process parameters (CPPs), and the yield of puerarin, total isoflavonoids, and extracta sicca were the critical quality attributes (CQAs). Models between CQAs and CPPs were constructed using both a conventional quadratic polynomial model (QPM) and the SVR algorithm. The results of the two models indicated that the SVR model had better performance, with a higher R2 and lower root-mean-square error (RMSE) and mean absolute deviation (MAD) than those of the QPM. Furthermore, the design space was predicted using a grid search technique. The operational range was extraction time, 24–51 min; number of extraction cycles, 3; and liquid–solid ratio, 14–18 mL/g. This study is the first reported work optimizing the design space of the extraction process of P. lobata based on an SVR model. SVR modeling, with its better prediction accuracy and generalization ability, could be a reliable tool for predicting the design space and shows great potential for the quality control of QbD.
Trichosanthis Pericarpium Aqueous Extract Protects H9c2 Cardiomyocytes from Hypoxia/Reoxygenation Injury by Regulating PI3K/Akt/NO Pathway
Authors: Chu Donghai , Zhang Zhenqiu
Published: September 2018

Trichosanthis Pericarpium (TP) is a traditional Chinese medicine for treating cardiovascular diseases. In this study, we investigated the effects of TP aqueous extract (TPAE) on hypoxia/reoxygenation (H/R) induced injury in H9c2 cardiomyocytes and explored the underlying mechanisms. H9c2 cells were cultured under the hypoxia condition induced by sodium hydrosulfite for 30 min and reoxygenated for 4 h. Cell viability was measured by MTT assay. The amounts of LDH, NO, eNOS, and iNOS were tested by ELISA kits. Apoptotic rate was detected by Annexin V-FITC/PI staining. QRT-PCR was performed to analyze the relative mRNA expression of Akt, Bcl-2, Bax, eNOS, and iNOS. Western blotting was used to detect the expression of key members in the PI3K/Akt pathway. Results showed that the pretreatment of TPAE remarkably enhanced cell viability and decreased apoptosis induced by H/R. Moreover, TPAE decreased the release of LDH and expression of iNOS. In addition, TPAE increased NO production and Bcl-2/Bax ratio. Furthermore, the mRNA and protein expression of p-Akt and eNOS were activated by TPAE pretreatment. On the contrary, a specific inhibitor of PI3K, LY294002 not only inhibited TPAE-induced p-Akt/eNOS upregulation but alleviated its anti-apoptotic effects. In conclusion, results indicated that TPAE protected against H/R injury in cardiomyocytes, which consequently activated the PI3K/Akt/NO signaling pathway.
An Ab Initio QM/MM Study of the Electrostatic Contribution to Catalysis in the Active Site of Ketosteroid Isomerase
Authors: Wang Xianwei , He Xiao
Published: September 2018

The electric field in the hydrogen-bond network of the active site of ketosteroid isomerase (KSI) has been experimentally measured using vibrational Stark effect (VSE) spectroscopy, and utilized to study the electrostatic contribution to catalysis. A large gap was found in the electric field between the computational simulation based on the Amber force field and the experimental measurement. In this work, quantum mechanical (QM) calculations of the electric field were performed using an ab initio QM/MM molecular dynamics (MD) simulation and electrostatically embedded generalized molecular fractionation with conjugate caps (EE-GMFCC) method. Our results demonstrate that the QM-derived electric field based on the snapshots from QM/MM MD simulation could give quantitative agreement with the experiment. The accurate calculation of the electric field inside the protein requires both the rigorous sampling of configurations, and a QM description of the electrostatic field. Based on the direct QM calculation of the electric field, we theoretically confirmed that there is a linear correlation relationship between the activation free energy and the electric field in the active site of wild-type KSI and its mutants (namely, D103N, Y16S, and D103L). Our study presents a computational protocol for the accurate simulation of the electric field in the active site of the protein, and provides a theoretical foundation that supports the link between electric fields and enzyme catalysis.
Unravelling the Distribution of Secondary Metabolites in Olea europaea L.: Exhaustive Characterization of Eight Olive-Tree Derived Matrices by Complementary Platforms (LC-ESI/APCI-MS and GC-APCI-MS)
Authors: Olmo-García Lucía , Kessler Nikolas , Neuweger Heiko , Wendt Karin , Olmo-Peinado José María , Fernández-Gutiérrez Alberto , Baessmann Carsten , Carrasco-Pancorbo Alegría
Published: September 2018

In order to understand the distribution of the main secondary metabolites found in Olea europaea L., eight different samples (olive leaf, stem, seed, fruit skin and pulp, as well as virgin olive oil, olive oil obtained from stoned and dehydrated fruits and olive seed oil) coming from a Picudo cv. olive tree were analyzed. All the experimental conditions were selected so as to assure the maximum coverage of the metabolome of the samples under study within a single run. The use of LC and GC with high resolution MS (through different ionization sources, ESI and APCI) and the annotation strategies within MetaboScape 3.0 software allowed the identification of around 150 compounds in the profiles, showing great complementarity between the evaluated methodologies. The identified metabolites belonged to different chemical classes: triterpenic acids and dialcohols, tocopherols, sterols, free fatty acids, and several sub-types of phenolic compounds. The suitability of each platform and polarity (negative and positive) to determine each family of metabolites was evaluated in-depth, finding, for instance, that LC-ESI-MS (+) was the most efficient choice to ionize phenolic acids, secoiridoids, flavonoids and lignans and LC-APCI-MS was very appropriate for pentacyclic triterpenic acids (MS (−)) and sterols and tocopherols (MS (+)). Afterwards, a semi-quantitative comparison of the selected matrices was carried out, establishing their typical features (e.g., fruit skin was pointed out as the matrix with the highest relative amounts of phenolic acids, triterpenic compounds and hydroxylated fatty acids, and seed oil was distinctive for its high relative levels of acetoxypinoresinol and tocopherols).
Structural, Luminescent and Thermal Properties of Heteronuclear PdII–LnIII–PdII Complexes of Hexadentate N2O4 Schiff Base Ligand
Authors: Miroslaw Barbara , Cristóvão Beata , Hnatejko Zbigniew
Published: September 2018

New PdII–LnIII–PdII complexes of hexadentate N2O4 Schiff base ligand (H4L: N,N′-bis(2,3-dihydroxybenzylidene)-1,3-diamino-2,2-dimethylpropane) with Eu (1), Tb (2), Er (3) and Yb (4) ([Pd2Eu(H2L)2NO3](NO3)2∙2H2O∙2CH3OH 1, [Pd2Ln(H2L)2H2O](NO3)3∙3H2O, where Ln = Tb 2, Er 3, [Pd2Yb(H2L)2H2O](NO3)3∙5.5H2O 4) were synthesized and characterized structurally and physicochemically by thermogravimetry (TG), differential thermogravimetry (DTG), differential scanning calorimetry (DSC) and luminescence measurements. The compounds 1–4 are built of cationic heterometallic PdII–LnIII–PdII trinuclear units. The palladium(II) centers adopt a planar square geometry occupying the smaller N2O2 cavity of the Schiff base ligand. The lanthanide(III) is surrounded by two Schiff base ligands (eight oxygen atoms) and its coordination sphere is supplemented by a chelating bidentate nitrate ion in 1 or by a water molecule in 2–4. The complexes have a bent conformation along the PdII–LnIII–PdII line with valence angles in the ranges of 162–171°. The decomposition process of the complexes results in mixtures of: PdO, Pd and respective lanthanide oxides Eu2O3, Tb2O3, Tb4O7, Er2O3, Yb2O3. The luminescent measurements show low efficiency intramolecular energy transfer only in the complex of terbium(III) (2).
Complete Chloroplast Genome Sequence and Phylogenetic Analysis of Aster tataricus
Authors: Shen Xiaofeng , Guo Shuai , Yin Yu , Zhang Jingjing , Yin Xianmei , Liang Conglian , Wang Zhangwei , Huang Bingfeng , Liu Yanhong , Xiao Shuiming , Zhu Guangwei
Published: September 2018

We sequenced and analyzed the complete chloroplast genome of Aster tataricus (family Asteraceae), a Chinese herb used medicinally to relieve coughs and reduce sputum. The A. tataricus chloroplast genome was 152,992 bp in size, and harbored a pair of inverted repeat regions (IRa and IRb, each 24,850 bp) divided into a large single-copy (LSC, 84,698 bp) and a small single-copy (SSC, 18,250 bp) region. Our annotation revealed that the A. tataricus chloroplast genome contained 115 genes, including 81 protein-coding genes, 4 ribosomal RNA genes, and 30 transfer RNA genes. In addition, 70 simple sequence repeats (SSRs) were detected in the A. tataricus chloroplast genome, including mononucleotides (36), dinucleotides (1), trinucleotides (23), tetranucleotides (1), pentanucleotides (8), and hexanucleotides (1). Comparative chloroplast genome analysis of three Aster species indicated that a higher similarity was preserved in the IR regions than in the LSC and SSC regions, and that the differences in the degree of preservation were slighter between A. tataricus and A. altaicus than between A. tataricus and A. spathulifolius. Phylogenetic analysis revealed that A. tataricus was more closely related to A. altaicus than to A. spathulifolius. Our findings offer valuable information for future research on Aster species identification and selective breeding.
Differential Pharmacological Activities of Oxygen Numbers on the Sulfoxide Moiety of Wasabi Compound 6-(Methylsulfinyl) Hexyl Isothiocyanate in Human Oral Cancer Cells
Authors: Lee Min-Ju , Tseng Wen-Ser , Lai Jerry Cheng-Yen , Shieh Hui-Ru , Chi Chih-Wen , Chen Yu-Jen
Published: September 2018

6-(methylsulfinyl) hexyl isothiocyanate (6-MITC) is a naturally occurring compound isolated from Wasabia japonica (wasabi). The synthetic derivatives, 6-(methylsulfenyl) hexyl isothiocyanate (I7447) and 6-(methylsulfonyl) hexyl isothiocyanate (I7557), were derived from 6-MITC with the deletion and addition of oxygen, respectively. We aimed to evaluate the effect of these synthetic compounds on human oral cancer cells, SAS and OECM-1. All three compounds (I7447, 6-MITC, and I7557) inhibited the viability of SAS and OECM-1 cells using MTT assay. Morphological observations showed various proportions of mitotic arrest and apoptosis in cells treated with these compounds. Cell cycle analysis revealed relatively abundant G2/M arrest in 6-MITC and I7557-treated cells, whereas sub-G1 accumulation was found in I7447-treated cells. In using phosphorylated histone H3 as a marker for mitosis, the addition of 6-MITC and I7557 (excluding I7447) could be shown to arrest cells during mitosis. In contrast, I7447 induced more prominent apoptosis than the 6-MITC or I7557 compounds. The down-regulated expression of the phosphorylated form of CHK1 and Cdc25c was noted in 6-MITC and I7557-treated cells. I7557 could sensitize SAS cells to death by radiation. The wasabi compound, 6-MITC, and its chemical derivatives with different numbers of oxygen may have differential pharmacological effects on human oral cancer cells.
Effect of Sensory Feedback on Turn-Taking Using Paired Devices for Children with ASD
Authors: Nunez Eleuda , Matsuda Soichiro , Hirokawa Masakazu , Yamamoto Junichi , Suzuki Kenji
Published: September 2018

Most children can naturally engage in play and by this, develop skills while interacting with their peers and toys. However, children with Autism Spectrum Disorder (ASD) often show impairments in play skills which result in limited opportunities for interaction with others and the learning of social skills. In this regard, robotic devices/toys that can provide simple and attractive indications are advantageous to engage children with ASD in play activities that require social and interaction skills. This project proposes a new interaction method using paired robotic devices called COLOLO to facilitate a fundamental exchange of intention in communication so-called turn-taking. These tangible devices are designed to sense the user’s manipulation, send a message to the paired device, and display visual cues for assisting children to achieve turn-taking through play. On the sessions with COLOLO there are two devices, one held by the therapist and one by the child, and they take turns to manipulate the toys and change their colors. In this article, two experimental conditions or interaction rules: the “two-sided lighting rule” and the “one-sided lighting rule" were introduced. The two interactions rules differ from each on the way the devices used the visual cues to indicate the turn-holder. The effect of each interaction rule on children’s turn-taking behaviors was investigated through an experimental study with four children with ASD. From the results, we found that with the one-sided lighting rule participants tended to shift their gaze more and to decrease the failed attempts of turn-taking. The discussion covers the possibilities of using paired devices to describe participants’ behaviors related to turn-taking quantitatively.
Correction: Chatterjee, A.; et al. Transition Metal Hollow Nanocages as Promising Cathodes for the Long-Term Cyclability of Li–O2 Batteries. Nanomaterials 2018, 8, 308
Authors: Chatterjee Amrita , Or Siu , Cao Yulin
Published: September 2018

The authors wish to add the following information to this paper [...]
Antibacterial Activity of Fructose-Stabilized Silver Nanoparticles Produced by Direct Current Atmospheric Pressure Glow Discharge towards Quarantine Pests
Authors: Dzimitrowicz Anna , Motyka-Pomagruk Agata , Cyganowski Piotr , Babinska Weronika , Terefinko Dominik , Jamroz Piotr , Lojkowska Ewa , Pohl Pawel , Sledz Wojciech
Published: September 2018

Development of efficient plant protection methods against bacterial phytopathogens subjected to compulsory control procedures under international legislation is of the highest concern having in mind expensiveness of enforced quarantine measures and threat of the infection spread in disease-free regions. In this study, fructose-stabilized silver nanoparticles (FRU-AgNPs) were produced using direct current atmospheric pressure glow discharge (dc-APGD) generated between the surface of a flowing liquid anode (FLA) solution and a pin-type tungsten cathode in a continuous flow reaction-discharge system. Resultant spherical and stable in time FRU-AgNPs exhibited average sizes of 14.9 ± 7.9 nm and 15.7 ± 2.0 nm, as assessed by transmission electron microscopy (TEM) and dynamic light scattering (DLS), respectively. Energy dispersive X-ray spectroscopy (EDX) analysis revealed that the obtained nanomaterial was composed of Ag while selected area electron diffraction (SAED) indicated that FRU-AgNPs had the face-centered cubic crystalline structure. The fabricated FRU-AgNPs show antibacterial properties against Erwinia amylovora, Clavibacter michiganensis, Ralstonia solanacearum, Xanthomonas campestris pv. campestris and Dickeya solani strains with minimal inhibitory concentrations (MICs) of 1.64 to 13.1 mg L−1 and minimal bactericidal concentrations (MBCs) from 3.29 to 26.3 mg L−1. Application of FRU-AgNPs might increase the repertoire of available control procedures against most devastating phytopathogens and as a result successfully limit their agricultural impact.
Commencing Nutrient Supplements before Full Enteral Feed Volume Achievement Is Beneficial for Moderately Preterm to Late Preterm Low Birth Weight Babies: A Prospective, Observational Study
Authors: Fan Wei Qi , Gan Amy , Crane Olivia
Published: September 2018

The aim of this study was to observe after following a routine change in the feeding protocol whether the earlier introduction of nutrient supplements improved nutritional outcomes in moderately preterm to late preterm low birth weight (LBW) babies. In this prospective observational study, LBW babies between 31 and 39 weeks’ gestation admitted to a Special Care Nursery were assigned to two groups (F80, n = 45, F160, n = 42) upon commencing nutrient supplement at total fluid intake achievement of 80 or 160 mL/kg/day. Outcomes included weight, protein intake, biochemical markers, feeding intolerance, and length of stay (LOS). F80 nutrient supplements commenced before F160 (2.8 vs. 6.7 days, p < 0.0001) and lasted longer (15.2 vs. 12.2 days, p < 0.03). Weight gain velocity and LOS were similar. F80 mean protein intake during the first 10 days was higher (3.38 vs. 2.74 g/kg/day, p < 0.0001). There were fewer infants with protein intake <3 g/kg/day in the F80 group (8% vs. 65%, p < 0001). F80 babies regained birthweight almost two days earlier (7.5 vs. 9.4 days, p < 0.01). Weight gain Z-scores revealed an attenuation of the trend towards lower weight percentiles in the F80 group. Feeding intolerance was decreased for F80 (24.4% vs. 47.6%, p < 0.03). There were no adverse outcomes. Earlier nutrient supplementation for LBW babies lifts mean protein intake to above 3 g/kg/day and reduces both the duration of post-birth weight loss and incidence of feeding intolerance.
Sodium Citrate Increases Expression and Flux of Mg2+ Transport Carriers Mediated by Activation of MEK/ERK/c-Fos Pathway in Renal Tubular Epithelial Cells
Authors: Takashina Yui , Manabe Aya , Hasegawa Hajime , Matsunaga Toshiyuki , Endo Satoshi , Ikari Akira
Published: September 2018

A chronic magnesium deficiency may be one of the causes of lifestyle-related diseases such as hypertension and diabetes. Serum Mg2+ concentration is strictly controlled by the reabsorption pathway in the renal tubules, but little is known about how Mg2+ reabsorption is upregulated. We searched for food compounds which can increase the expression levels of Mg2+ transport carriers including transient receptor potential melastatin 6 (TRPM6) channel and cyclin M2 (CNNM2). Sodium citrate (SC) increased the mRNA levels of TRPM6 and CNNM2 in renal tubular epithelial NRK-52E cells. The SC-induced elevation of TRPM6 was inhibited by U0126, a mitogen-activated protein kinase kinase (MEK) inhibitor, but the CNNM2 was not. SC increased the levels of p-ERK1/2 and p-c-Fos, which were inhibited by U0126. SC induced alkalization of culture medium. Both SC and alkalization enhanced Mg2+ influx, which was inhibited by U0126 and introduction of TRPM6 siRNA. The reporter activity of TRPM6 was increased by SC and alkalization, which was suppressed by mutation in an AP-1-binding site. The SC-induced elevation of p-ERK1/2 and p-EGFR was inhibited by diphenylene iodonium, a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor, and erlotinib, an epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor. SC did not change the level of acetyl histone H3, but increased the association of c-Fos with the promoter region of TRPM6. These results suggest that SC increases TRPM6 expression and Mg2+ influx mediated by the activation of NADPH oxidase and an EGFR/ERK/c-Fos pathway in the renal tubules.
Effect of Arbuscular Mycorrhizal Fungi on Pratylenchus penetrans Infestation in Apple Seedlings under Greenhouse Conditions
Authors: Ceustermans An , Van Hemelrijck Wendy , Van Campenhout Jelle , Bylemans Dany
Published: September 2018

A major problem in fruit cultivation in Flanders is replant disease due to a lack of uncultivated soils available for new plantings. Replant disease can cause poor growth and affect time to full production, however Arbuscular Mycorrhizal Fungi (AMF) can prove their usefulness with regard to these problems. To further investigate the effect of AMF on nematodes, different AMF species were amended to potted apple seedlings in the presence of the nematode Pratylenchus penetrans. Generally, apple seedlings grew better in the presence of nematodes when mycorrhiza were inoculated into the soil. Moreover, a positive correlation (R2 ≥ 0.88) was found between the percentage root length colonization of the roots of apple seedlings, by AMF species, and nematode reduction in the soil of the seedlings. Indigenous AMF could colonize the roots of apple seedlings the most efficiently, resulting in a higher biocontrol effect. Besides, a synergistic effect was observed when two AMF strains were applied together leading to a significant growth response of the seedlings.
The Impact of Heat Stress and Water Deficit on the Photosynthetic and Stomatal Physiology of Olive (Olea europaea L.)—A Case Study of the 2017 Heat Wave
Authors: Haworth Matthew , Marino Giovanni , Brunetti Cecilia , Killi Dilek , De Carlo Anna , Centritto Mauro
Published: September 2018

Heat waves are predicted to increase in frequency and duration in many regions as global temperatures rise. These transient increases in temperature above normal average values will have pronounced impacts upon the photosynthetic and stomatal physiology of plants. During the summer of 2017, much of the Mediterranean experienced a severe heat wave. Here, we report photosynthetic leaf gas exchange and chlorophyll fluorescence parameters of olive (Olea europaea cv. Leccino) grown under water deficit and full irrigation over the course of the heat wave as midday temperatures rose over 40 °C in Central Italy. Heat stress induced a decline in the photosynthetic capacity of the olives consistent with reduced ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) activity. Damage to photosystem II was more apparent in plants subject to water deficit. In contrast to previous studies, higher temperatures induced reductions in stomatal conductance. Heat stress adversely affected the carbon efficiency of olive. The selection of olive varieties with enhanced tolerance to heat stress and/or strategies to mitigate the impact of higher temperatures will become increasingly important in developing sustainable agriculture in the Mediterranean as global temperatures rise.
Mechanical Behavior—Microstructure Relationships in Injection-Molded Polyamide 66
Authors: Billon Noëlle , Giraudeau Joan , Bouvard Jean , Robert Gilles
Published: September 2018

Clear relationships between the semi-crystalline microstructure of injection molding polymers and their mechanical behavior are not yet totally established for all polymers. Part of this relative lack of understanding is because an unambiguous scientific approach is difficult to build up. The processing of samples promotes a microstructure which is not uniform and can be described in various ways on different scales. This introduces uncertainty in the correlations. Most completed studies were conducted on polyolefin, which exhibits an evolution of microstructure that is quite easy to observe and to correlate to mechanical properties. This paper intends to illustrate a more diffuse case. To achieve this, combined characterizations along the flow path and throughout the thickness of a plaque as well as characterizations of the local microstructure and tensile behavior of polyamide 66 are described. The microstructure was explored in terms of skin-core structure, spherulites sizes, crystallinity ratio and lamellae organization. Mechanical properties were addressed with non-monotonic tests with the use of DIC (Digital Image Correlation) to assess true behavior. The effect of humidity is also accounted for. It is demonstrated that small changes in lamellae or interlamellar amorphous phase are likely to be responsible for non-uniform mechanical properties, whereas more macroscopic levels (skin core structure, spherulites level of crystallinity ratio) appeared to be irrelevant levels of description. Consequently, the usual simplified analyses based on optical microscopy and differential scanning calorimetry (DSC) can be inefficient in improving knowledge in that field.
Thin Film Composite and/or Thin Film Nanocomposite Hollow Fiber Membrane for Water Treatment, Pervaporation, and Gas/Vapor Separation
Authors: Khulbe Kailash , Matsuura Takeshi
Published: September 2018

Thin film composite (TFC) polymeric hollow fiber (HF) membranes are widely used in industrial gas/vapor separations and water treatment. There are many advantages of TFC HF membranes, such as low energy requirements, simplicity of operation, and high specificity. In the present article, a review is made on the progress that has been achieved during the past 15 years in the preparation of the HF substrate and the preparation/modification of the thin selective layer. The review also includes their applications in water treatment, dehydration of alcohols via pervaporation, and gas/vapor separation.
Nanocomposite Film Containing Fibrous Cellulose Scaffold and Ag/TiO2 Nanoparticles and Its Antibacterial Activity
Authors: Li Yanxiang , Tian Jessica , Yang Chuanfang , Hsiao Benjamin
Published: September 2018

Cellulose is a natural polymer that is widely used in daily life, but it is susceptible to microorganism growth. In this study, a simple sol–gel technique was utilized to incorporate the cellulose scaffold with Ag/TiO2 nanoparticles. The morphology and crystal structure of the as-prepared Ag/TiO2/cellulose composite film were characterized using scanning electron microscopy (SEM) and X-ray diffraction (XRD) methods. Antibacterial tests involving the use of Escherichia coli (E. coli) were carried out under dark and UV-light conditions to evaluate the efficiency of the Ag/TiO2/cellulose composite film in comparison with pristine cellulose paper and TiO2/cellulose composite film. The results indicated that the antibacterial activity of the Ag/TiO2/cellulose composite film outperformed all other samples, where the Ag content of 0.030 wt% could inhibit more than 99% of E. coli. This study suggests that finely dispersed nanocale Ag/TiO2 particles in the cellulose scaffold were effective at slowing down bacterial growth, and the mechanisms of this are also discussed.
Acknowledgment of Country: Intersecting Australian Pentecostalisms Reembeding Spirit in Place
Authors: Riches Tanya
Published: September 2018

This article builds upon a previous application of Nimi Wariboko’s “Charismatic City” proposal, adapting it to the Australian context. Within this metaphor, the Pentecostal worshipper is situated in a rhizomatic network that flows with particular energies, forming a new spirit-ed common space that serves as the basis of global civil society. In this network, the culturally dominant metropolis and the culturally alternative heteropolis speak in distinct voices or tongues: An act that identifies and attunes participants to the Spirit’s existing work in the world. Here, two interweaving Australian Pentecostalisms are presented. The metropolis in this example is Hillsong Church, well known for its song repertoire and international conferences. In contrast, the heteropolis is a diverse group led by Aboriginal Australian pastors Will and Sandra Dumas from Ganggalah Church. In 2017, Hillsong Conference incorporated a Christianised version of an “Acknowledgement to Country,” a traditional Indigenous ceremonial welcome, into its public liturgy, which is arguably evidence of speaking new languages. In this case, it also serves a political purpose, to recognise Aboriginal Pentecostals within a new commons. This interaction shows how Joel Robbin’s Pentecostal “impulses” of “globalization,” “cultural fragmentation” and “world-making” can operate simultaneously within the ritual life of national churches.
Three Different Ways Synchronization Can Cause Contagion in Financial Markets
Authors: Massad Naji , Andersen Jørgen Vitting
Published: September 2018

We introduce tools to capture the dynamics of three different pathways, in which the synchronization of human decision-making could lead to turbulent periods and contagion phenomena in financial markets. The first pathway is caused when stock market indices, seen as a set of coupled integrate-and-fire oscillators, synchronize in frequency. The integrate-and-fire dynamics happens due to “change blindness”, a trait in human decision-making where people have the tendency to ignore small changes, but take action when a large change happens. The second pathway happens due to feedback mechanisms between market performance and the use of certain (decoupled) trading strategies. The third pathway occurs through the effects of communication and its impact on human decision-making. A model is introduced in which financial market performance has an impact on decision-making through communication between people. Conversely, the sentiment created via communication has an impact on financial market performance. The methodologies used are: agent based modeling, models of integrate-and-fire oscillators, and communication models of human decision-making.
Kinematics Analysis of a Class of Spherical PKMs by Projective Angles
Authors: Legnani Giovanni , Fassi Irene
Published: September 2018

This paper presents the kinematics analysis of a class of spherical PKMs Parallel Kinematics Machines exploiting a novel approach. The analysis takes advantage of the properties of the projective angles, which are a set of angular conventions of which their properties have only recently been presented. Direct, inverse kinematics and singular configurations are discussed. The analysis, which results in the solution of easy equations, is developed at position, velocity and acceleration level.