bluuebunny/crossref_metadata_2025 huggingface dataset:
A Normative Feedback Intervention on Gambling Behavior—A Longitudinal Study of Post-Intervention Gambling Practices in At-Risk Gamblers
Authors: Berge Jonas , Abrahamsson Tove , Lyckberg Axel , Franklin Katja , Håkansson Anders
Published: March 2022

Background

In problem gambling, normative personalized feedback interventions have demonstrated promising effects. Given the widespread increase in online gambling in recent years, internet-delivered normative feedback may serve as a promising intervention. This study aimed to examine whether such an intervention, delivered by a gambling operator and aiming to help problem gamblers decrease their gambling, may in fact be associated with lower gambling practices post-intervention.

Methods

Online questions on norms and beliefs about one's own and peers' gambling habits, derived from the Gambling Quantity and Perceived Norms Scale, were followed by personalized feedback, delivered online by the Swedish state-owned gambling operator. A total of 1,453 gamblers consented to participate in a pre-post measure of wagering levels.

Results

Wagering decreased significantly post-intervention (28 days) compared to pre-intervention (28 days prior). The decrease was significantly more pronounced in younger and online casino gamblers. In an 84-day follow-up, the decrease remained significant, although less pronounced.

Conclusions

An online normative intervention delivered by a state-owned gambling operator, addressing norms and beliefs about gambling levels, may lower risky gambling in the short term. Implications and further research needs are discussed.
Association Between Early Parental Death and Loneliness in Adulthood: A Community-Based Study in Southwest China
Authors: Peng Anjiao , Lai Wanlin , He Shixu , Li Wanling , Song Tingting , Ji Shuming , Zhao Xia , Chen Lei
Published: March 2022

Loneliness is a growing public health problem that threatens physical and mental health to a large extent. Compelling evidence has shown that premature parental death is strongly associated with many mental health disorders in adulthood, but whether it increases the risk of loneliness remains unclear. In this large community-based study, we included 32,682 adult participants (20–93 years old) from Southwest China and used the three-item short version of University of California, Los Angeles, Loneliness Scale to identify participants with loneliness. A total of 1,975 participants reported loneliness, which resulted in a loneliness prevalence of 6.0% in Southwest China. Logistic regression was used to evaluate the association between early parental death and loneliness after adjusting for age, gender, education level, marital status, smoking and drinking status, living status, and chronic diseases. We found that early parental death was significantly associated with loneliness [odds ratio (OR) = 1.21, 95% confidence interval (CI), 1.03–1.42]. A Sensitivity analysis excluding those with mental health disorders (796 participants) yielded similar results (OR = 1.26, 95% CI = 1.06–1.49). We also found that being younger, single, divorced, or widowed, and more educated; living alone; and having chronic disorders were associated with loneliness. We conclude that childhood parental death is associated with loneliness in adulthood, suggesting the need for early intervention in affected children to prevent long-term adverse neuropsychiatric effects.
Unraveling the Complexity of Cardiac Distress: A Study of Prevalence and Severity
Authors: Jackson Alun C. , Rogerson Michelle C. , Amerena John , Smith Julian , Hoover Valerie , Alvarenga Marlies E. , Higgins Rosemary O. , Grande Michael R. Le , Ski Chantal F. , Thompson David R. , Murphy Barbara M.
Published: March 2022

Introduction

While much research attention has been paid to anxiety and depression in people who have had a recent cardiac event, relatively little has focused on the broader concept of cardiac distress. Cardiac distress is a multidimensional construct that incorporates but extends beyond common mood disorders such as anxiety and depression. In the present study we assessed the prevalence, severity and predictors of a broad range of physical, affective, cognitive, behavioral and social symptoms of cardiac distress. This is the first study to investigate cardiac distress in this comprehensive way.

Method

A sample of 194 patients was recruited from two hospitals in Australia. Eligible participants were those who had recently been hospitalized for an acute cardiac event. Data were collected at patients' outpatient clinic appointment ~8 weeks after their hospital discharge. Using a questionnaire developed through a protocol-driven 3-step process, participants reported on whether they had experienced each of 74 issues and concerns in the past 4 weeks, and the associated level of distress. They also provided sociodemographic and medical information. Regression analyses were used to identify risk factors for elevated distress.

Results

Across the 74 issues and concerns, prevalence ratings ranged from a high of 66% to a low of 6%. The most commonly endorsed items were within the domains of dealing with symptoms, fear of the future, negative affect, and social isolation. Common experiences were “being physically restricted” (66%), “lacking energy” (60%), “being short of breath” (60%), “thinking I will never be the same again” (57%), and “not sleeping well” (51%). While less prevalent, “not having access to the health care I need,” “being concerned about my capacity for sexual activity,” and “being unsupported by family and friends” were reported as highly distressing (74, 64, and 62%) for those experiencing these issues. Having a mental health history and current financial strain were key risk factors for elevated distress.

Conclusion and Implications

Specific experiences of distress appear to be highly prevalent in people who have had a recent cardiac event. Understanding these specific fears, worries and stressors has important implications for the identification and management of post-event mental health and, in turn, for supporting patients in their post-event cardiac recovery.
The Cost Effectiveness of Donafenib Compared With Sorafenib for the First-Line Treatment of Unresectable or Metastatic Hepatocellular Carcinoma in China
Authors: Meng Rui , Cao Yingdan , Zhou Ting , Hu Hongfei , Qiu Yijin
Published: March 2022

Background

Recent clinical trials have demonstrated that donafenib has superior efficacy and safety compared with sorafenib in Chinese patients with unresectable or metastatic hepatocellular carcinoma (HCC). The objective of this study was to assess the cost effectiveness of donafenib compared with sorafenib for the treatment of patients with unresectable or metastatic HCC in China.

Methods

A three-state partitioned survival model was developed to perform a cost-effectiveness analysis comparing donafenib and sorafenib from a Chinese healthcare payer's perspective. The model adopted a lifetime horizon and a 4-week cycle length. Survival data were derived from the ZGDH3 study and fitted with standard parametric functions for extrapolation beyond the trial period. Cost data were obtained from the mean price of publicly listed online bids in 2021 and medical service prices across provinces in China. Utility data were obtained from previous literature. The cost and health outcomes were discounted at an annual rate of 5%. Deterministic and probabilistic sensitivity analyses (PSAs) were carried out to verify the robustness of the model.

Results

Compared with sorafenib, donafenib incurred a higher cost (US$22,330.23 vs. US$14,775.92) but yielded more quality-adjusted life years (1.045 vs. 0.861 QALYs). The incremental cost-effectiveness ratio (ICER) for donafenib was US$41,081.52 per QALY gained (ICER = US$13,439.10/QALY). The PSA results indicated that at a willingness-to-pay threshold of 3 times the GDP in China, the probability of donafenib being cost effective was 16.9%. The ICER (US$13,439.10/QALY) decreased when the branded price of sorafenib was used in the model.

Conclusions

Donafenib is unlikely to be cost effective compared with sorafenib for the first-line treatment of unresectable or metastatic HCC in China. Reducing the price of donafenib can increase the possibility of it being cost effective in the future.
The Survey of the Health of Wisconsin (SHOW) Program: An Infrastructure for Advancing Population Health
Authors: Malecki Kristen M. C. , Nikodemova Maria , Schultz Amy A. , LeCaire Tamara J. , Bersch Andrew J. , Cadmus-Bertram Lisa , Engelman Corinne D. , Hagen Erika , McCulley Laura , Palta Mari , Rodriguez Allison , Sethi Ajay K. , Walsh Matt C. , Nieto F. Javier , Peppard Paul E.
Published: March 2022

Introduction

The Survey of the Health of Wisconsin (SHOW) was established in 2008 by the University of Wisconsin (UW) School of Medicine and Public Health (SMPH) with the goals of (1) providing a timely and accurate picture of the health of the state residents; and (2) serving as an agile resource infrastructure for ancillary studies. Today, the SHOW program continues to serve as a unique and vital population health research infrastructure for advancing public health.

Methods

SHOW currently includes 5,846 adult and 980 minor participants recruited between 2008 and 2019 in four primary waves. WAVE I (2008–2013) includes annual statewide representative samples of 3,380 adults ages 21 to 74 years. WAVE II (2014–2016) is a triannual statewide sample of 1,957 adults (age ≥18 years) and 645 children (age 0–17). WAVE III (2017) consists of follow-up of 725 adults from the WAVE I and baseline surveys of 222 children in selected households. WAVEs II and III include stool samples collected as part of an ancillary study in a subset of 784 individuals. WAVE IV consists of 517 adults and 113 children recruited from traditionally under-represented populations in biomedical research including African Americans and Hispanics in Milwaukee, Wisconsin.

Findings to Date

The SHOW resource provides unique spatially granular and timely data to examine the intersectionality of multiple social determinants and population health. SHOW includes a large biorepository and extensive health data collected in a geographically diverse urban and rural population. Over 60 studies have been published covering a broad range of topics including, urban and rural disparities in cardio-metabolic disease and cancer, objective physical activity, sleep, green-space and mental health, transcriptomics, the gut microbiome, antibiotic resistance, air pollution, concentrated animal feeding operations and heavy metal exposures.

Discussion

The SHOW cohort and resource is available for continued follow-up and ancillary studies including longitudinal public health monitoring, translational biomedical research, environmental health, aging, microbiome and COVID-19 research.
Exploring the Challenges of Afghan Refugee Women Facing COVID-19: A Qualitative Study in Iran
Authors: Yoosefi Lebni Javad , Enayat Halime , Irandoost Seyed Fahim , Dehghan Ali Akbar
Published: March 2022

Objective

Afghan refugee women in Iran confront many problems in dealing with COVID-19 due to their fragile conditions. Therefore, the aim of this study was to explore the challenges of Afghan refugee women in the face of COVID-19 in Iran with a qualitative approach.

Methods

The present study was conducted with a qualitative approach among Afghan refugee women in Iran. Data were collected through semi-structured face-to-face and telephone interviews and were saturated with 30 women. Both targeted and snowball sampling were used. Data were analyzed using conventional qualitative content analysis and Graneheim and Lundman method. Guba and Lincoln criteria were observed to evaluate the quality of research results.

Results

143 primary codes, 12 subcategories and five main categories were obtained from data analysis. The main categories include little knowledge and information (limited access to information resources, incomplete knowledge about COVID-19), family challenges (intensified experience of violence and conflict in the family, problems related to childbirth and pregnancy), socio-economic challenges (exacerbation of economic problems, high-risk living conditions, social isolation, limited support of social and health organizations), health issues (problems related to treatment, injustice in providing services and facilities) and problems after the death of a COVID-19 patient (burial challenges for immigrants; lack of funeral rites).

Conclusion

Afghan refugee women in Iran are very vulnerable facing COVID-19 due to their fragile conditions. Social and health institutions and organizations need to provide more support to these women so that they can protect their health and that of their families against COVID-19 and the damage caused by it.
Approximation to the Consumption of Healthcare Resources and the Economic Cost of SARS-CoV-2 Patient Management: A Retrospective Study
Authors: Calderón-Moreno Jesús , Juárez-Vela Raúl , Delgado-Rodríguez María Jesús , Quintana-Díaz Manuel , Magallón-Botaya Rosa , Olivan-Blázquez Bárbara , Cobos-Rincón Ana , Santolalla-Arnedo Iván , Ramírez-Torres Carmen Amaia , Gea-Caballero Vicente , Andrés-Esteban Eva María
Published: March 2022

Spain has become one of the countries most affected by coronavirus disease 2019 (COVID-19), with the highest testing rates, and one of the worst-performing countries in the fight against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. There are no studies related to the consumption of health resources and the economic cost of the SARS-CoV-2 virus. We present a retrospective analysis of 9,811 (Primary Care and Hospital) patients which aimed to estimate public health expenditure by the consumption of health resources due to COVID-19. According to the results, the gender distribution of patients has a similar rate in both groups, with slightly higher rates in women. Similarly, age is the same in both groups, with a median of 62 years in the case of hospitalizations and 61 years in the case of primary care; using a weighted average of these rates and costs, we can estimate that the average cost of care per patient infected with the SARS-CoV-2 virus, regardless of the course is €2373.24. We conclude that a patient with COVID-19 without hospitalization costs €729.79, while the expenses of a hospitalized patient are between €4294.36 and €14440.68, if there is ICU admission.
What Is a Leaf?
Authors: Mendelson Ellie , Zumajo-Cardona Cecilia , Ambrose Barbara A.
Published: March 2022

When thinking of plants, the color green inevitably comes to mind. As perhaps the most noticeable and easily recognizable part of the natural world, leaves decorate the world green. If you take a close look at the plants in your yard or on your street, it is likely that you will not find two that are exactly the same. This is largely thanks to the enormous amount of diversity that exists in leaf shapes, textures, and even colors! All leaves share general characteristics that we will review in this article, but some have taken on special abilities. Some leaves are modified to look like flowers, others are modified to help the plant climb, to protect the plant from potential threats, or even to act as insect traps. We want to share with you the extraordinary world of leaves: keep reading, and you will learn the many shapes, structures, and incredible functions of leaves.
The Impact of the Coronavirus Disease - 19 Pandemic on the Clinical Characteristics and Treatment of Adult Patients with Acute Appendicitis
Authors: An Sanghyun , Kim Hae-Rim , Jang Sungwoo , Kim Kwangmin
Published: March 2022

Purpose

This study aimed to investigate the characteristics, severity, and treatment of adult patients with acute appendicitis in Korea over a 2-year period during the coronavirus disease (COVID-19) pandemic compared to those before the pandemic. We also investigated whether there were any changes in clinical characteristics of acute appendicitis before and after vaccination against the coronavirus.

Methods

We retrospectively reviewed the medical records of patients who were diagnosed with acute appendicitis at our institution between March 1, 2019, and August 31, 2021. We divided the patients into three groups (pre-pandemic, before vaccination, and after vaccination) and analyzed the clinical outcomes.

Results

The time from symptom onset to hospital arrival and the time from symptom onset to operation increased during the COVID-19 pandemic period compared to the pre-pandemic period. The rate of complicated appendicitis during the pandemic was higher than that before the pandemic. In addition, the number of new daily cases showed a positive correlation with the time from symptom onset to hospital arrival (OR, 0.03; 95% CI, 0.02 to 0.04; P < 0.001) and complicated appendicitis (OR, 1.002; 95% CI, 1.001–1.002; P = 0.0017). The vaccination rate showed a negative correlation with the time from symptom onset to hospital arrival (OR, −2.26; 95% CI, −3.42 to −1.11; P < 0.001) and complicated appendicitis (OR, 0.915; 95% CI, 0.84 to 0.996; P = 0.0404).

Conclusions

Employing hospital-wide efforts, such as screening by rapid PCR testing, to avoid further time delays, and nationwide efforts, such as vaccination, to shorten the time from symptom onset to hospital arrival, are necessary to maintain the quality of treatment of acute appendicitis during an infectious disease pandemic.
Thermographic Evaluation of the Duration of Skin Cooling After Cryotherapy in Dogs Following Tibial Plateau Leveling Osteotomy Surgery
Authors: Woo Sang Chul , Lee Jack , Millis Darryl L. , Drum Marti G.
Published: March 2022

Objective

To evaluate the duration of cooling after cryotherapy on the skin over stifle joints in dogs after tibial plateau leveling osteotomy (TPLO) surgery using thermography.

Materials and Methods

Seventeen client-owned dogs of various breeds were enrolled in the study. Dogs underwent TPLO surgeries, and on the next day, thermal imaging was performed on the operated stifle prior to cryotherapy for baseline. Orthogonal views were repeated at 30-min intervals until the skin over the stifle had thermally equilibrated. An ice pack was applied for 20 min on the medial and lateral aspects of the stifle. Each stifle was then re-imaged every 15 min for the first 60 min then every 30 min subsequently until the temperature was within 1°C of the pre-cryotherapy temperature.

Results

Mean skin temperature of the medial view showed no significance difference compared to baseline value at 45 min after cryotherapy was discontinued and after 60 min for the lateral and cranial views. Mean skin temperature was overall higher in the medial view compared to the lateral and cranial during the rewarming period (except immediately after cold application). Mean skin temperatures of all views combined showed a significant decrease in temperature during cryotherapy application, with a slow increase until a plateau was reached after 45 min of rewarming.

Conclusions and Clinical Relevance

Dogs undergoing TPLO for cranial cruciate ligament injury showed quicker rewarming period of superficial tissues compared to previous studies. Cryotherapy is a beneficial modality to reduce superficial tissue temperature in dogs undergoing TPLO, acknowledging that these dogs may require more frequent cryotherapy post-operatively due to more rapid rewarming time compared to dogs without surgery.
The Finding of the Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) in a Wild Eurasian River Otter (Lutra lutra) Highlights the Need for Viral Surveillance in Wild Mustelids
Authors: Padilla-Blanco Miguel , Aguiló-Gisbert Jordi , Rubio Vicente , Lizana Víctor , Chillida-Martínez Eva , Cardells Jesús , Maiques Elisa , Rubio-Guerri Consuelo
Published: March 2022

Animals have been involved in the three known outbreaks of severe respiratory syndromes due to coronaviruses (years 2005, 2012, and 2019). The pandemic nature of the SARS-CoV-2 outbreak increases the likelihood of infection from humans of susceptible animal species that, thus, could become secondary viral hosts and even disease reservoirs. We present evidence of spillover infection of wild mustelids by reporting the presence of SARS-CoV-2 in a Eurasian river otter found near a water reservoir in the Valencian Community (Spain). We detected the virus using two different commercial RTqPCR assays on RNA extracted from the nasopharynx (swabbing) and from lung tissue and mediastinal lymph node homogenates. The corresponding samples from two additional otters from distant sites tested negative in identical assays. The diagnosis in the positive otter was confirmed by two-tube RT-PCR assay in which RNA was first retrotranscribed, and then specific regions of the spike (S), nucleocapsid (N), and ORF10 genes were separately amplified from the produced cDNA, followed by electrophoretic visualization and Sanger sequencing. The sequences of the amplified products revealed some non-synonymous changes in the N and ORF10 partial sequences, relative to the consensus sequence. These changes, identified already in human patient samples, point to human origin of the virus, although their specific combination was unique. These findings, together with our previous report of SARS-CoV-2 infection of feral American mink, highlight the need for SARS-CoV-2 surveillance of wild or feral mustelids to evaluate the risk that these animals could become SARS-CoV-2 reservoirs.
Repeatability and Predictability of Calf Feeding Behaviors—Quantifying Between- and Within-Individual Variation for Precision Livestock Farming
Authors: Carslake Charles , Occhiuto Francesca , Vázquez-Diosdado Jorge A. , Kaler Jasmeet
Published: March 2022

Individual calves show substantial between- and within-individual variation in their feeding behavior, the existence and extent of which are not fully researched. In this study, 57,196 feeding records, collected by a computerized milk feeder from 48 pre-weaned calves over 5 weeks, were collated and analyzed for individual differences in three different feeding behaviors using a multi-level modeling approach. For each feeding behavior, we quantified behavioral variation by calculating repeatability and the coefficient of variation in predictability. Our results indicate that calves differed from each other in their average behavioral expression (behavioral type) and in their residual, within individual variation around their behavioral type (predictability). Feeding rate and total meals had the highest repeatability (>0.4) indicating that substantial, temporally stable between-individual differences exist for these behaviors. Additionally, for some behaviors (e.g., feeding rate) calves varied from more to less predictable whereas for other behaviors (e.g., meal size) calves were more homogenous in their within-individual variation around their behavioral type. Finally, we show that for individual calves, behavioral types for feeding rate and total meals were positively correlated which may suggest the existence of an underlying factor responsible for driving the (co)expression of these two behaviors. Our results highlight how the application of methods from the behavioral ecology literature can assist in improving our understanding of individual differences in calf feeding behavior. Furthermore, by uncovering consistencies between individual behavioral differences in calves, our results indicate that animal personality may play a role in driving variability in calf feeding behavior.
The Inclusion of Alhagi maurorum in Growing Camel Diet: Effect on Performance, Liver-Related Blood Metabolites, and Antioxidant Status
Authors: Ghavipanje Navid , Vargas-Bello-Pérez Einar , Afshin Mojtaba , Hosseini Seyyed Ahmad , Aghashahi Alireza , Vatankhah Amir Mansour
Published: March 2022

This study determined the effect of dietary inclusion of camelthron [Alhagi maurorum (AM)] on the performance, blood metabolites, and antioxidant status of growing camels. A total of 18 Sindhi camel calves of 9–10 months of age and 115 ± 7 kg body weight (BW) were randomly assigned to three diets (with a forage:concentrate ratio of 50:50) that were formulated by partial and total substitution of alfalfa hay with AM as follows: (1) diet without AM (control), (2) diet containing 25% of AM (AM-25), and (3) diet containing 50% of AM (AM-50) (dry matter basis) for 150 days. Dry matter intake (DMI) was recorded daily. The camels were weighed individually on days 0, 30, 60, 90, 120, and 150. Blood samples were collected on days 0, 75, and 150. DMI was increased (p = 0.004) with AM-50 feeding followed by AM-25. Total weight gain (p = 0.048) and average daily gain (ADG) (p = 0.043) decreased with AM-50; however, no differences were observed between the AM-25 and CON groups. Feed cost per kg BW gain tended to decrease (p = 0.092) and return per kg BW gain tended to increase (p = 0.087) by AM feeding. The plasma triglycerides (TGs) (p = 0.046) and cholesterol (CHOL) (p = 0.025) concentration were reduced with AM inclusion. Additionally, the AM50-fed camels showed the lowest concentration of aspartate aminotransferase (AST) (p = 0.008) and alanine aminotransferase (ALT) (p = 0.0036), followed by AM-25. The plasma malondialdehyde (MDA) was depressed (p = 0.037) and total antioxidant capacity (TAC) was enhanced (p = 0.016) with both the AM-25 and AM-50. Moreover, feeding the AM containing diets led to higher (p = 0.004) glutathione peroxidase (GPx) along with a tendency for superoxide dismutase (SOD) (p = 0.075) and catalase (CAT) (p = 0.094). Overall, feeding camels with AM for up to 25% of their dry matter (DM) diet positively influenced the antioxidant status without severe deleterious effects on performance.
Tryptophan Alleviates Acute Heat Stress-Induced Impairment of Antioxidant Status and Mitochondrial Function in Broilers
Authors: Ouyang Jingxin , Zhou Hua , Li Qiufen , Zheng Jun , Chen Chun , Guo Shuaipeng , You Jinming , Li Guanhong
Published: March 2022

Heat stress has been considered as a critical risk factor for decreasing performance and causing oxidative stress in broilers. The tryptophan (TRP) derivative 5-hydroxytryptophan has been reported to protect membrane fluidity in broilers suffering from oxidative stress. Therefore, this experiment was conducted to investigate the effects of dietary TRP supplementation on antioxidant status and mitochondrial function-related genes expressions in broilers exposed to acute heat stress (34 ± 1°C, 24 h). Female Arbor Acres broilers (19-d-old, n = 180) were randomly assigned to 1 of 3 treatments. Broilers were fed a basal diet and in the thermoneutral conditions (TN, 23 ± 1°C) was considered as the TN group. Broilers were fed a basal diet and exposed to acute heat stress (HS, 34 ± 1°C) was regarded as the HS group. Broilers were fed a basal diet supplemented with 0.18% L-tryptophan and under HS conditions was treated as the HS + TRP groups. Heat stress led to increased malondialdehyde (MDA) concentration (P < 0.05), while it elevated catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and total antioxidant capacity activities (T-AOC) (P < 0.05) compared with the TN group. Nevertheless, compared with the HS group, TRP supplementation increased SOD activity (P < 0.05). The effects of acute heat stress were associated with increased mRNA abundance for redox-related genes (P < 0.05), and reduced mRNA levels for mitochondrial function-related genes (P < 0.05). Notably, the effects of acute heat stress on mitochondrial function-related genes expressions were reversed by TRP treatment. Collectively, dietary 0.18% TRP supplementation beneficially protects against acute heat stress-induced oxidation stress and mitochondrial dysfunction by regulating antioxidant states and increasing mitochondrial function-related genes expressions in broilers.
Impact of the COVID-19 Pandemic on Human Papillomavirus Vaccination in Brazil
Authors: Silva Tércia Moreira Ribeiro Da , Nogueira de Sá Ana Carolina Micheletti Gomide , Beinner Mark Anthony , Abreu Mery Natali Silva , Matozinhos Fernanda Penido , Sato Ana Paula Sayuri , Vieira Ed Wilson Rodrigues
Published: March 2022

Objective: To analyze the number of applied HPV vaccine doses before (from April 2019 to March 2020) and after (from April 2020 to September 2020) social distancing measures in response to the COVID-19 pandemic in states and regions of Brazil.

Methods: Ecological time-series study, using data from the Brazilian National Immunization Program (PNI). Using the Mann-Whitney test, we evaluated the difference between the median number of applied doses during the periods April 2019 to March 2020 and from April 2020 to September 2020. Spatial analysis identified clusters with a high or low percentage reduction in the median applied doses. Prais-Winsten regression models identified temporal trends in the applieddoses from both periods.

Results: There was a significant reduction in the median HPV vaccine doses applied, formation of spatial clusters and, after a sharp drop in the number of applieddoses during the months following social distancing. There was a tendency to increase the applied vaccines doses.

Conclusion: The COVID-19 pandemic resulted in reduction of the number of HPV vaccine doses applied as a possible effect of restrictive measures caused by the pandemic.
How Can We Introduce ART into Wild Felid Conservation in Practice? Joint Experience in Semen Collection from Captive Wild Felids in Europe
Authors: Prochowska Sylwia , Niżański Wojciech , Snoeck Feline , Wydooghe Eline , Van Soom Ann , Kochan Joanna , Stefanyk Vasyl
Published: March 2022

Although artificial reproductive techniques (ART) are considered to be a valuable tool for species conservation, information about their introduction into clinical practice for wild felids is limited. The aim of this paper was to jointly describe cases of non-experimental sperm collection from males of various species of wild felids, performed by three European centers focused on feline reproduction. In total, the article presents 22 attempts of semen collection in 12 species of wild felids. The reasons for semen collection were: fertility assessment (10 cases), artificial insemination (5 cases), sperm rescue (postmortem collection for cryopreservation, 5 cases), and sperm banking (in vivo collection for cryopreservation, 2 cases). Semen collection was successful (defined as at least 1 × 106 spermatozoa) in 15 cases. The failures in obtaining spermatozoa were most probably due to (1) male infertility, (2) wrong age/non-breeding season, or (3) recent multiple copulations. The cases presented here confirm that although ART have been introduced into clinical practice, they are mostly used in cases of infertility, not as routine breeding tools. Higher involvement of zoological gardens and private breeders is required, as many chances for preservation of valuable material are lost.
Insights from Koala–Cattle Interaction Experiments: Koalas and Cattle May See Each Other as a Disturbance
Authors: Jiang Alex Zijian , Tribe Andrew , Phillips Clive J. C. , Murray Peter J.
Published: March 2022

Koalas are facing many threats and have now been officially listed as endangered. Recently, concerns were raised in anecdotal reports of koalas being killed by livestock, especially cattle. We investigated the significance of cattle as a threat to koala survival via two koala–cattle interaction experiments, from both the koala and cattle perspectives. In the first experiment, we recorded the ranging behaviour of free-ranging, radio-collared koalas prior to, during and after cattle grazed within their usual home range. Koalas decreased their distance travelled and the size of their home range when they shared space with cattle, compared with the period before cattle started grazing within their home range. In the second experiment, we recorded the reactions of cattle towards koalas that they encountered on the ground, using motorised animal models: a model koala mounted on a remote-controlled vehicle and a model dog mounted on the same vehicle, and the vehicle alone. The koala model elicited aggression and fear in cattle, similar to the dog model, whereas their reaction to the vehicle was significantly less aggressive. No actual attacks by the cattle were observed. The results provide experimental evidence that negative koala–livestock interactions occur and indicate that cattle and koalas may see each other as a disturbance.
The Occurrence of Skin Mites from the Demodecidae and Psorergatidae (Acariformes: Prostigmata) Families in Bats, with a Description of a New Species and New Records
Authors: Cierocka Karolina , Izdebska Joanna N. , Rolbiecki Leszek , Ciechanowski Mateusz
Published: March 2022

The bat skin mites from the closely-related Demodecidae and Psorergatidae families occur synhospitally, populating the same host species and perhaps neighboring microhabitats. However, data on their occurrence and parasitism are fragmentary and dispersed. Thus far, 27 Demodecidae and 18 Psorergatidae species have been described, but the coexistence of mites from both families was only demonstrated in six species of bats. This article presents a description of Demodex pusillus sp. nov. from Nyctalus noctula, including a new host record (first observation of demodecid mites in Nyctalus) and a new record concerning the occurrence of Psorergatoides kerivoluae in Plecotus auritus. It also includes an updated global checklist of the occurrence of Demodecidae and Psorergatidae in Chiroptera, including data on their records/distribution and location in their hosts. In both studied families, the mites exhibit preferences, and even topographic specificity, colonizing different microhabitats in the host, including the eye region (e.g., Meibomian glands of the eyes, corneal surface and eyelid vault), wing membranes and hairy skin on the body. Such colonization of separate microhabitats enables different species to co-occur within the same host, while the total number of parasites determines the level of parasite load, with higher levels being associated with the incidence of disease symptoms. It is worth mentioning that Demodex pusillus sp. nov. is the smallest known representative of the Demodecidae family and one of the smallest animals (70–80 micrometers in length).
Expression of Anti-Müllerian Hormone and Its Type 2 Receptor in the Ovary of Pregnant and Cyclic Domestic Cats
Authors: Gültiken Nilgün , Yarim Murat , Aslan Selim , Gürler Hande , Yarim Gul Fatma , Tuncay Müge , İnal Sinem , Schäfer-Somi Sabine
Published: March 2022

To evaluate the expression of AMH and its receptor AMHRII, ovaries of 33 p cats were investigated by western blot and immunohistochemistry. After ovariohysterectomy, the cats were grouped according to pregnancy stages and ovarian/placental endocrine activity: group I (n = 3, 24–29 days), II (n = 8, 32–40 days), III (n = 4, 41–46 days), IV (n = 6, 53–61 days) and according to cycle stages: V (n = 6, interestrus) and VI (n = 6, estrus). Serum progesterone- and AMH-concentration was measured. Follicle numbers did not differ between groups. The number of corpora lutea was higher in pregnant cats than in the non-pregnant cats. Serum AMH concentration was at maximum between day 30 and 50 of gestation, and was higher than in non-pregnant cats, then decreased towards term (p < 0.05). In the ovaries, AMH immunopositivity was observed in granulosa cells of secondary and antral follicles, and in interstitial cells of corpora lutea; highest percentage of immunopositive areas was detected in group III (p < 0.05). A positive correlation between the number of corpora lutea and the positive AMH signals in ovarian tissue was determined (r2 = 0.832, p < 0.05); however, only during mid-gestation (group II). Expression of AMHRII was in close co-localization with AMH and strong in the interstitial cells surrounding follicles undergoing atresia. AMHRII expression did not differ between pregnant groups but was higher compared to estrus cats (p ˂ 0.05). We conclude that AMH and AMHRII expression in the feline ovary is comparable to other species. The high serum AMH concentration and ovarian AMHRII expression between day 30 and 50 of gestation are probably related to ovarian activity and follicular atresia.
Modeling Effects of Atmospheric Nitrogen Deposition on the Water Quality of the MR-SNWDP
Authors: Wang Jiabiao , Cai Siyu , Zhao Jianshi
Published: March 2022

Modeling the effects of atmospheric deposition on the water quality of the middle route of the South-to-North Water Diversion Project (MR-SNWDP) can provide a basis for scientific protection and optimal allocation of water resources. Considering the total nitrogen (TN) as a representative water quality index, this study analyzed the water quality variations along the MR-SNWDP and in the Miyun Reservoir under atmospheric deposition, using the hydrodynamic and environmental numerical models. The results in 2016 revealed that the atmospheric deposition significantly affected the water quality of the MR-SNWDP, and the effects of atmospheric deposition on the water quality of the middle route and Miyun Reservoir showed different variation rules. However, the clearer water from the MR-SNWMP would significantly deteriorate after storage in the Miyun Reservoir as the TN concentration increased significantly. Based upon the scenario analysis, it was found that the constant water diversion scheme helped reduce the effects of atmospheric deposition. Further, it is recommended that the storage amounts in the Miyun Reservoir should be as low as possible.
First Year of Biophysica
Authors: Buck Matthias , Muñoz Victor
Published: March 2022

“I can’t believe another year has passed already” is what most of us think when another birthday is upon us or when we see our children grow [...]
Early Diagnosis and Diagnostic Delay in Oral Cancer
Authors: Varela-Centelles Pablo
Published: March 2022

Oral cancer is a very serious public health problem in many parts of the world, particularly in developing countries, where about two-thirds of incident cases occur [...]
Prognostic Impact of Circulating Methylated Homeobox A9 DNA in Patients Undergoing Treatment for Recurrent Ovarian Cancer
Authors: Faaborg Louise , Andersen Rikke Fredslund , Waldstrøm Marianne , Henriksen Jon Røikjær , Adimi Parvin , Jakobsen Anders , Steffensen Karina Dahl
Published: March 2022

Methylated Homeobox A9 circulating tumor DNA (meth-HOXA9) has been suggested as a blood-based biomarker in epithelial ovarian cancer (EOC), although its prognostic significance remains unproven. The aim of the present study was to investigate the prognostic impact of meth-HOXA9 in patients with recurrent EOC. DNA was purified from 4 mL plasma and, following bilsulfite conversion, meth-HOXA9 was analyzed using a methylation-specific droplet digital PCR. Detection of meth-HOXA9 was reported as a percentage of total DNA and as a binary variable (detectable and undetectable). Meth-HOXA9 status and its dynamics during palliative treatment were correlated with overall survival (OS) as the primary endpoint. At baseline, meth-HOXA9 was detected in 65.9% (83/126) of the patients. The median OS was 8.9 and 17.9 months in patients with detectable and undetectable meth-HOXA9 at baseline (hazard ratio: 2.04, p = 0.002), which remained significant in the multivariate analysis. Median OS in patients with an increase in meth-HOXA9 after one treatment cycle was 5.3 months compared to 33 months in patients with undetectable meth-HOXA9 (p < 0.001). Meth-HOXA9 was significantly related to poor survival and may serve as a prognostic marker in patients with recurrent EOC. The longitudinal monitoring of meth-HOXA9 is clinically feasible with the perspective of aiding clinical decision making.
Plasma-Beam Processing of Tools Made of SiAlON Dielectric Ceramics to Increase Wear Resistance When Cutting Nickel–Chromium Alloys
Authors: Metel Alexander , Volosova Marina , Mustafaev Enver , Melnik Yury , Seleznev Anton , Grigoriev Sergey
Published: March 2022

This research aimed at an increase in wear resistance of round cutting plates manufactured with SiAlON dielectric ceramics through deposition of wear-resistant coatings. To increase effectiveness of the coatings, their adhesion was improved by the removal of defective surface layers from the cutting plates before the deposition. As the depth of caverns and grooves appearing on the cutting plates due to manufacturing by diamond grinding reached 5 µm, a concentrated beam of fast argon atoms was used for the removal of defective layers with a thickness exceeding the depth of caverns and grooves. At the equal angles of incidence to the front and back surfaces of the cutting wedge amounting to 45 degrees, two-hour-long etching of rotating cutting plates provided removal of defective layers with thickness of ~10 µm from the surfaces. After the removal, the cutting edge radius of the plates diminished from 20 to 10 µm, which indicates the cutting plates’ sharpening. Wear-resistant TiAlN coatings deposited after the etching significantly improve the processing stability and increase wear resistance of the cutting plates by not less than 1.7 times.
Chemical Doping of a Silica Matrix with a New Organic Dye from the Group of Heterocyclic Compounds—Chemical, Optical and Surface Characteristics
Authors: Wojtasik Katarzyna , Zięba Magdalena , Wojtasik Michał , Tyszkiewicz Cuma , Pokladko-Kowar Monika , Gondek Ewa , Danel Andrzej , Karasiński Paweł
Published: March 2022

This paper presents the results of research on a luminescent dye bound in a silica matrix. The new developed dye from the group of azaheterocyclic compounds was used: 3-(p-hydroxyphenyl)-1-phenyl-1H-pyrazolo [3,4-b]quinoxaline. The structure and composition of the dye was examined by 1HNMR, 13CNMR, FTIR, and elemental analysis. Its absorption and photoluminescence characteristics were tested in solvents of different polarity in UV-Vis range. The films were prepared by sol–gel method and dip-coating technique. The dye was introduced into a sol in the course of a synthesis of the latter. DLS and FTIR measurements of sols were performed. Optical properties were investigated using UV-Vis spectrophotometry and monochromatic ellipsometry. The surface morphology of the layers was examined by atomic force microscopy. Our investigations showed that the dye bound in the silica matrix does not lose its photoluminescent properties. The emission band at λPL = 550 nm (λex = 365 nm) was recorded for the dye in the matrix. The layers are optically homogeneous with smooth surfaces. Dye doped silica films have RMS surface roughness of 2.17 nm over areas of 2 × 2 μm2. The idea of binding a photoluminescent dye in a silica matrix presented in the paper can be applied in the technology of luminescent solar concentrators.
The Impact of COVID-19 on Academic Cancer Clinical Trials in Canada and the Initial Response from Cancer Centers
Authors: Sundquist Stephen , Kato Diana , Xu Rebecca Y. , Schoales James , Kulendran Saranya , Dancey Janet E.
Published: March 2022

The COVID-19 pandemic resulted in temporary holds placed on new trial startups, patient recruitment and follow up visits for trials which contributed to major disruptions in cancer center trial unit operations. To assess the impact, the Canadian Cancer Clinical Trials Network (3CTN) members participated in regional meetings and a survey to understand the impact of the pandemic to academic cancer clinical trials (ACCT) activity, cancer trial unit operations and supports needed for post-pandemic recovery. Trial performance and recruitment data collected from 1 April 2020–31 March 2021 was compared to the same period in previous years. From 1 April–30 June 2020, patient recruitment decreased by 67.5% and trial site activations decreased by 81% compared to the same period in 2019. Recovery to reopening and recruitment of ACCTs began after three months, which was faster than initially projected. However, ongoing COVID-19 impacts on trial unit staffing and operations continue to contribute to delayed trial activations, lower patient recruitment and may further strain centers’ capacity for participation in academic-sponsored trials.
Implication of ERBB2 as a Predictive Tool for Survival in Patients with Pancreatic Cancer in Histological Studies
Authors: Ortega Miguel A. , Pekarek Leonel , Fraile-Martinez Oscar , Garcia-Montero Cielo , Saez Miguel A. , Asúnsolo Angel , Alvarez-Mon Miguel A. , Monserrat Jorge , Ruiz-Llorente Lidia , García-Honduvilla Natalio , Albillos Agustin , Buján Julia , Alvarez-Mon Melchor , Guijarro Luis G.
Published: March 2022

Pancreatic cancer will be positioned by the year 2030 as the second cause of oncological death after lung cancer. The pathophysiology of the most common variety, which involves the adenocarcinoma of the pancreas, represents one of the main challenges for current oncology to explain its tumorigenesis and create a targeted treatment. The tumor microenvironment, metastatic capacity, and lack of early diagnosis lead patients to present advanced stages at the time of diagnosis. Despite numerous efforts, little progress has been made in clinical outcomes and with respect to the improved survival of these patients. For this reason, in recent years, numerous diagnostic tests, treatments, and possible approaches in the fields of radiotherapy, chemotherapy, immunotherapy, and surgery have been developed to find a combination of methods that improves life expectancy in patients diagnosed with this disease. On the other hand, the scientific community has made numerous advances in the molecular bases of pancreatic cancer since several oncogenetic pathways have been described and the markers expressed by the tumor have proven to be useful in the prognosis of pancreatic adenocarcinoma. These molecular alterations allow the study of possible therapeutic targets that improve the prognosis of these patients, but even numerous tumor cell-individual interactions must be explained to understand the underlying pathophysiology causing the high mortality. Therefore, the purpose of our study is to examine the expression of markers such as EGFR, Cyclin D1, andCDK4 in order to find a relationship with the possible long-term prognostic factors of patients affected by pancreatic ductal adenocarcinoma. Our results show that there is a prognostic role for ErbB2, EGFR, beta catenin, cyclin D1, and CDK4. Of these, we highlight the clinical importance of ErbB2 in the survival rates of patients who overexpress this component.