bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcribing Genes the Hard Way: In Vitro Reconstitution of Nanoarchaeal RNA Polymerase Reveals Unusual Active Site Properties
Authors: Nottebaum Sven , Weinzierl Robert O. J.
Published: June 2021

Nanoarchaea represent a highly diverged archaeal phylum that displays many unusual biological features. The Nanoarchaeum equitans genome encodes a complete set of RNA polymerase (RNAP) subunits and basal factors. Several of the standard motifs in the active center contain radical substitutions that are normally expected to render the polymerase catalytically inactive. Here we show that, despite these unusual features, a RNAP reconstituted from recombinant Nanoarchaeum subunits is transcriptionally active. Using a sparse-matrix high-throughput screening method we identified an atypical stringent requirement for fluoride ions to maximize its activity under in vitro transcription conditions.
A High-Protein, Low Glycemic Index Diet Suppresses Hunger but Not Weight Regain After Weight Loss: Results From a Large, 3-Years Randomized Trial (PREVIEW)
Authors: Zhu Ruixin , Fogelholm Mikael , Larsen Thomas M. , Poppitt Sally D. , Silvestre Marta P. , Vestentoft Pia S. , Jalo Elli , Navas-Carretero Santiago , Huttunen-Lenz Maija , Taylor Moira A. , Stratton Gareth , Swindell Nils , Kaartinen Niina E. , Lam Tony , Handjieva-Darlenska Teodora , Handjiev Svetoslav , Schlicht Wolfgang , Martinez J. Alfredo , Seimon Radhika V. , Sainsbury Amanda , Macdonald Ian A. , Westerterp-Plantenga Margriet S. , Brand-Miller Jennie , Raben Anne
Published: June 2021

Background: Previous studies have shown an increase in hunger during weight-loss maintenance (WLM) after diet-induced weight loss. Whether a combination of a higher protein, lower glycemic index (GI) diet and physical activity (PA) can counteract this change remains unclear.

Aim: To compare the long-term effects of two diets [high protein (HP)-low GI vs. moderate protein (MP)-moderate GI] and two PA programs [high intensity (HI) vs. moderate intensity (MI)] on subjective appetite sensations during WLM after ≥8% weight loss (WL).

Methods: Data derived from the 3-years PREVIEW randomized intervention study. An 8-weeks WL phase using a low-energy diet was followed by a 148-weeks randomized WLM phase. For the WLM phase, participants were assigned to one of the four groups: HP-MI, HP-HI, MP-MI, and MP-HI. Available data from 2,223 participants with overweight or obesity (68% women; BMI ≥ 25 kg/m2). Appetite sensations including satiety, hunger, desire to eat, and desire to eat something sweet during the two phases (at 0, 8 weeks and 26, 52, 104, and 156 weeks) were assessed based on the recall of feelings during the previous week using visual analogue scales. Differences in changes in appetite sensations from baseline between the groups were determined using linear mixed models with repeated measures.

Results: There was no significant diet × PA interaction. From 52 weeks onwards, decreases in hunger were significantly greater in HP-low GI than MP-moderate GI (Ptime × diet = 0.018, Pdietgroup = 0.021). Although there was no difference in weight regain between the diet groups (Ptime × diet = 0.630), hunger and satiety ratings correlated with changes in body weight at most timepoints. There were no significant differences in appetite sensations between the two PA groups. Decreases in hunger ratings were greater at 52 and 104 weeks in HP-HI vs. MP-HI, and greater at 104 and 156 weeks in HP-HI vs. MP-MI.

Conclusions: This is the first long-term, large-scale randomized intervention to report that a HP-low GI diet was superior in preventing an increase in hunger, but not weight regain, during 3-years WLM compared with a MP-moderate GI diet. Similarly, HP-HI outperformed MP-HI in suppressing hunger. The role of exercise intensity requires further investigation.

Clinical Trial Registration:www.ClinicalTrials.gov, identifier: NCT01777893.
Inflammatory Myofibroblastoma of the Breast: A Case Report
Authors: Wei Ling , Jiang Guoyuan , Bai Lala , Li Tingchao , Ma Xuejin , Jiang Lin , Wang Jie , Li Shiguang
Published: June 2021

Inflammatory myofibroblastic tumor (IMT) is a rare tumor with low-grade malignant risk mainly occurring in soft tissues and lungs, and it is extremely rare in the breast. Meanwhile, imaging findings of the tumor often present with non-specific features that lead to misdiagnosis and delayed treatment. Here, we report a case of inflammatory myofibroblastic tumor in the breast with the imaging findings of mammography, magnetic resonance imaging (MRI), and pathologic findings to improve the understanding of the disease. The patient was treated by surgical operation, and was followed up for 44 months, no local recurrence and distant metastasis.
The Efficacy and Safety of Esmolol for Septic Shock: A Systematic Review and Meta-analysis of Randomized Controlled Trials
Authors: Huang Po , Zheng Xiangchun , Liu Zhi , Fang Xiaolei
Published: June 2021

Purpose: The meta-analysis aims to identify whether septic shock patients can benefit from esmolol.

Materials and Methods: The relevant studies from MEDLINE, Cochrane Library, Embase were searched by two independent investigators using a variety of keywords. Stata software (version 12.0, Stata Corp LP, College Station, TX, United States)was used for statistical analysis.

Results: A total of 14 studies were identified and incorporated into the meta-analysis. For overall analysis, the treatment of esmolol was associated with decreased 28-day mortality (RR = 0.66, 95% CI = 0.56–0.77, p < 0.001). Meanwhile, our analysis found that, esmolol could decrease HR (SMD: −1.70; 95% CI: [−2.24−(−1.17)], cTnI (SMD: −1.61; 95% CI: [−2.06−(−1.16)] compared with standard treatment. No significant differences between the two groups were found in MAP, Lac, CI, and SVI.

Conclusion: The findings of this meta-analysis intend to demonstrate that septic shock patients with high heart beats rate might be benefit from esmolol treatment despite enough fluid resuscitation. While, dependent on the study published, with the further development of septic shock, the positive impact of esmolol varies. The appropriate heart rate change interval cannot be confirmed, further high-quality and large-scale RCTs should be performed to verify it and screening more suitable heart rate levels.

Systematic Review Registration: CRD42021239513
CPTM Symmetry for the Dirac Equation and Its Extended Version Based on the Vector Representation of the Lorentz Group
Authors: Marsch E. , Narita Y.
Published: June 2021

We revisit the CPT theorem for the Dirac equation and its extended version based on the vector representation of the Lorentz group. Then it is proposed that CPTM may apply to this fundamental equation for a massive fermion a s a singlet or a doublet with isospin. The symbol M stands here for reversing the sign of the mass in the Dirac equation, which can be accomplished by operation on it with the so-called gamma-five matrix that plays an essential role for the chirality in the Standard Model. We define the CPTM symmetry for the standard and extended Dirac equation and discuss its physical implications and some possible consequences for general relativity.
Highly Efficient and Comprehensive Identification of Ethyl Methanesulfonate-Induced Mutations in Nicotiana tabacum L. by Whole-Genome and Whole-Exome Sequencing
Authors: Udagawa Hisashi , Ichida Hiroyuki , Takeuchi Takanori , Abe Tomoko , Takakura Yoshimitsu
Published: June 2021

Tobacco (Nicotiana tabacum L.) is a complex allotetraploid species with a large 4.5-Gb genome that carries duplicated gene copies. In this study, we describe the development of a whole-exome sequencing (WES) procedure in tobacco and its application to characterize a test population of ethyl methanesulfonate (EMS)-induced mutations. A probe set covering 50.3-Mb protein coding regions was designed from a reference tobacco genome. The EMS-induced mutations in 19 individual M2 lines were analyzed using our mutation analysis pipeline optimized to minimize false positives/negatives. In the target regions, the on-target rate of WES was approximately 75%, and 61,146 mutations were detected in the 19 M2 lines. Most of the mutations (98.8%) were single nucleotide variants, and 95.6% of them were C/G to T/A transitions. The number of mutations detected in the target coding sequences by WES was 93.5% of the mutations detected by whole-genome sequencing (WGS). The amount of sequencing data necessary for efficient mutation detection was significantly lower in WES (11.2 Gb), which is only 6.2% of the required amount in WGS (180 Gb). Thus, WES was almost comparable to WGS in performance but is more cost effective. Therefore, the developed target exome sequencing, which could become a fundamental tool in high-throughput mutation identification, renders the genome-wide analysis of tobacco highly efficient.
Integration of Gas Exchange With Metabolomics: High-Throughput Phenotyping Methods for Screening Biostimulant-Elicited Beneficial Responses to Short-Term Water Deficit
Authors: Antonucci Giulia , Croci Michele , Miras-Moreno Begoña , Fracasso Alessandra , Amaducci Stefano
Published: June 2021

Biostimulants are emerging as a feasible tool for counteracting reduction in climate change-related yield and quality under water scarcity. As they are gaining attention, the necessity for accurately assessing phenotypic variables in their evaluation is emerging as a critical issue. In light of this, high-throughput phenotyping techniques have been more widely adopted. The main bottleneck of these techniques is represented by data management, which needs to be tailored to the complex, often multifactorial, data. This calls for the adoption of non-linear regression models capable of capturing dynamic data and also the interaction and effects between multiple factors. In this framework, a commercial glycinebetaine- (GB-) based biostimulant (Vegetal B60, ED&F Man) was tested and distributed at a rate of 6 kg/ha. Exogenous application of GB, a widely accumulated and documented stress adaptor molecule in plants, has been demonstrated to enhance the plant abiotic stress tolerance, including drought. Trials were conducted on tomato plants during the flowering stage in a greenhouse. The experiment was designed as a factorial combination of irrigation (water-stressed and well-watered) and biostimulant treatment (treated and control) and adopted a mixed phenotyping-omics approach. The efficacy of a continuous whole-canopy multichamber system coupled with generalized additive mixed modeling (GAMM) was evaluated to discriminate between water-stressed plants under the biostimulant treatment. Photosynthetic performance was evaluated by using GAMM, and was then correlated to metabolic profile. The results confirmed a higher photosynthetic efficiency of the treated plants, which is correlated to biostimulant-mediated drought tolerance. Furthermore, metabolomic analyses demonstrated the priming effect of the biostimulant for stress tolerance and detoxification and stabilization of photosynthetic machinery. In support of this, the overaccumulation of carotenoids was particularly relevant, given their photoprotective role in preventing the overexcitation of photosystem II. Metabolic profile and photosynthetic performance findings suggest an increased effective use of water (EUW) through the overaccumulation of lipids and leaf thickening. The positive effect of GB on water stress resistance could be attributed to both the delayed onset of stress and the elicitation of stress priming through the induction of H2O2-mediated antioxidant mechanisms. Overall, the mixed approach supported by a GAMM analysis could prove a valuable contribution to high-throughput biostimulant testing.
Studying Dynamics of Human Information Gathering Behaviors Using Social Robots
Authors: Eshed Matan , Epstein Matan , Zohar Ada H. , Gordon Goren
Published: June 2021

A novel social interaction is a dynamic process, in which participants adapt to, react to and engage with their social partners. To facilitate such interactions, people gather information relating to the social context and structure of the situation. The current study aimed to deepen the understanding of the psychological determinants of behavior in a novel social interaction. Three social robots and the participant interacted non-verbally according to a pre-programmed “relationship matrix” that dictated who favored whom. Participants' gaze was tracked during the interaction and, using Bayesian inference models, resulted in a measure of participants' social information-gathering behaviors. Our results reveal the dynamics in a novel environment, wherein information-gathering behavior is initially predicted by psychological inflexibility and then, toward the end of the interaction, predicted by curiosity. These results highlight the utility of using social robots in behavioral experiments.
Understanding E-Commerce Consumers’ Repeat Purchase Intention: The Role of Trust Transfer and the Moderating Effect of Neuroticism
Authors: Jeon Hyeon Gyu , Kim Cheong , Lee Jungwoo , Lee Kun Chang
Published: June 2021

The dominant position of e-commerce is especially being articulated in the retailing industry once again due to several constraints that the world faces in the COVID-19 pandemic era. In this regard, this study explores the significant role of trust transfer (from offline to online) and the moderating effect of consumers’ neurotic traits in the framework of trust-satisfaction-repurchase intention in the e-commerce context based on a survey with 406 Korean e-commerce consumers. Moreover, a prediction-oriented segmentation (POS) technique combined with structural equation models (SEM) was utilized to reveal consumers’ probable hidden heterogeneous characteristics. The outcomes of the global model SEM analysis indicate that offline-online trust transference occurs in e-commerce, and the conveyed trust significantly influences satisfaction and consumers’ repeat purchase intention through satisfaction. Neuroticism also has significant positive effects on trust transfer in the global model. However, results in three subgroups generated by POS show heterogeneous characteristics that considerably differed from the global model test results. The implications from this study will be beneficial to field practitioners in the e-commerce industry in addressing the importance of trust transfer, negative neurotic traits as well as heterogeneous aspects of consumers.
Attitude of Medical Students About Their Role and Social Accountability in the COVID-19 Pandemic
Authors: Hong Jihoon , Jung Ikjae , Park Mingeol , Kim Kyumin , Yeo Sungook , Lee Joohee , Suh Sooyeon , Hong Youjin , Park Jangho , Chung Seockhoon
Published: June 2021

Background:In this study, we aimed to explore the attitude of medical students toward their role and social accountability in this pandemic era. An online survey was developed to elicit information on (1) the role of medical students in the pandemic era; (2) Medical education in the “new normal,” and (3) the impact of COVID-19 on medical students.

Methods:The online survey, developed by a team consisting of three medical students, three psychiatry residents, and three professors of psychiatry, was conducted on 574 participants (213 medical students, 180 graduates, and 181 professors) in the University of Ulsan College of Medicine, Seoul, South Korea. Anxiety symptom rating scales, including the Stress and Anxiety to Viral Epidemics-6 (SAVE-6) scale and the Generalized Anxiety Disorder−7 (GAD-7) scale, were applied to measure participant anxiety level.

Results:Medical students indicated their willingness to join the healthcare response to the COVID-19 pandemic, if requested; however, graduates and professors recommended that medical students continue their training rather than join the pandemic healthcare response. In the new normal era, medical education has had to change appropriately. Moreover, adequate knowledge of COVID-19 infection and spread must be considered for the continuation of clinical clerkships during the pandemic. Overall, medical students who indicated anxiety about treating possible or confirmed cases of COVID-19 rated higher on the SAVE-6 scale. Finally, medical students who reported that COVID-19 had an impact on their studies and daily life rated higher on the general anxiety scale (GAD-7).

Conclusion:Social accountability is an important issue for medical students in the pandemic era. At the same time, non-disruption of their academic calendar would ensure continuous availability of component medical professionals, which is important for adequate future healthcare responses.
Musculoskeletal Disorders Associated With Quality of Life and Body Composition in Urban and Rural Public School Teachers
Authors: Vega-Fernández Gustavo , Lera Lydia , Leyton Bárbara , Cortés Pilar , Lizana Pablo A.
Published: June 2021

Introduction: Teachers have been reported to be a labor group with high rates of musculoskeletal disorders (MSDs), stress, and strong deterioration of quality of life (QoL). However, little information exists about the association between MSD, QoL, and body composition in rural and urban teachers.

Objective: The aim was to study the association of MSD with QoL perception and body composition of urban and rural teachers.

Participants and Methods: Participants are comprised a representative sample of urban and rural public schoolteachers from the Valparaiso Region, Chile. MSDs were evaluated with the Standardized Nordic Questionnaire for Musculoskeletal Symptoms validated for the Chilean population. QoL perception was evaluated with the 36-Item Short-Form Survey (SF-36). Body composition was measured via bioimpedance. A logistic regression model was used to evaluate the association between MSD, QoL, and body composition, adjusted for age and gender.

Results: A total of 88.9% (urban 90%; rural 87%) of teachers felt pain in some body area, 71.2% of them with limitations; 39% of teachers presented body fat obesity, with the highest rate in rural women. The body area with the greatest MSD prevalence was the neck and shoulders (68.6%). Significant differences were observed between teachers with >p75 of MSD (over six pain regions) and those with ≤p75 (six or fewer painful regions; p < 0.05) on six QoL scales and on physical health components (PCSs) and mental health (MCS) in urban teachers. However, rural teachers presented no differences. The association between teachers with >p75 MSD and low QoL perception was significant (p < 0.05) in PCS and MCS. Furthermore, the regression model presents a significant association between rural areas and low PCS perception.

Conclusions: Urban and rural teachers present high rates of MSD and obesity. Teachers with higher rates of MSD have their mental and physical QoL affected, making workplace intervention in MSD necessary to prevent teacher health deterioration.
The Lived Experience of Inequalities in the Provision of Treatment for Hepatitis C
Authors: Skyrme Sarah Louise
Published: June 2021

The issues of health, illness, stigma and inequalities in healthcare provision, areas that in my role as a social researcher were already of interest and concern, shifted to a different perspective when I was diagnosed with hepatitis C. From this altered position, my body and lifeworld were a nexus point for a range of ongoing challenges around staying as well as possible, and the struggle to get my healthcare needs met. There is a gap between the support provided for some ill and disabled people, and the help that they actually require. This is particularly so for conditions that are not well understood, that have a low public profile, limited funding, and/or are in some way stigmatised due to perceived differences to social norms. Hepatitis C is one such condition, it is a viral disease that is transmitted through blood-to-blood contact and it causes ongoing damage to the liver. Because of the systemic nature of the disease, individuals may struggle to cope with the demands of work and daily living, and their lifeworld and opportunities are frequently limited. It can be challenging for the patient to advocate for themselves due to low energy levels, self-blame for getting ill, and the stigma associated with the condition. The first generation of effective anti-viral drugs emerged from clinical trials in 2013, but in the United Kingdom context, access was only possible for those with advanced liver disease. Therefore, many patients felt compelled to purchase the anti-virals through Buyers Clubs, whereby generic versions of the drugs are imported for personal use at a fraction of the market cost. In this article I draw on my own lived experience of joining a Buyers Club as an example of how risks and benefits are weighed, and to explain the contexts in which decisions are shaped and made.
The Irish Programme to Eradicate Bovine Viral Diarrhoea Virus—Organization, Challenges, and Progress
Authors: Graham David , More Simon J. , O'Sullivan Padraig , Lane Elizabeth , Barrett Damien , Lozano Jose-Maria , Thulke Hans-Hermann , Verner Sharon , Guelbenzu Maria
Published: June 2021

A mandatory national Irish bovine viral diarrhoea (BVD) eradication programme, coordinated by Animal Health Ireland, commenced in 2013. Key decisions and programme review are undertaken by a cross-industry Implementation Group (BVDIG) supported by a Technical Working Group. Ear notch tissue is collected from all new-born calves using modified official identity tags, supplemented by additional blood sampling, including for confirmatory testing of calves with initial positive results and testing of their dams. Testing is delivered by private laboratories in conjunction with the National Reference Laboratory, with all results reported to a central database. This database manages key elements of the programme, issuing results to herdowners by short message service messaging supplemented by letters; assigning and exchanging animal-level statuses with government databases of the Department of Agriculture, Food and the Marine to enable legislated restrictions on animal movements; assigning negative herd status based on test results; generating regular reports for programme management and evaluation and providing herd-specific dashboards for a range of users. Legislation supporting the programme has been in place throughout but has not thus far mandated the slaughter of persistently infected (PI) calves. A key challenge in the early years, highlighted by modeling, was the retention of PI animals by some herd owners. This has largely been resolved by measures including graduated financial supports to encourage their early removal, herd-level movement restrictions, ongoing programme communications and the input of private veterinary practitioners (PVPs). A framework for funded investigations by PVPs in positive herds was developed to identify plausible sources of infection, to resolve the status of all animals in the herd and to agree up to three measures to prevent re-introduction of the virus. The prevalence of PI calves in 2013 was 0.66%, within 11.3% of herds, reducing in each subsequent year, to 0.03 and 0.55%, respectively, at the end of 2020. Recent regulatory changes within the European Union for the first time make provision for official approval of national eradication programmes, or recognition of BVD freedom, and planning is underway to seek approval and, in due course, recognition of freedom within this framework by 2023.
An Introduction to Development of Centralized and Distributed Stochastic Approximation Algorithm with Expanding Truncations
Authors: Zhao Wenxiao
Published: June 2021

The stochastic approximation algorithm (SAA), starting from the pioneer work by Robbins and Monro in 1950s, has been successfully applied in systems and control, statistics, machine learning, and so forth. In this paper, we will review the development of SAA in China, to be specific, the stochastic approximation algorithm with expanding truncations (SAAWET) developed by Han-Fu Chen and his colleagues during the past 35 years. We first review the historical development for the centralized algorithm including the probabilistic method (PM) and the ordinary differential equation (ODE) method for SAA and the trajectory-subsequence method for SAAWET. Then, we will give an application example of SAAWET to the recursive principal component analysis. We will also introduce the recent progress on SAAWET in a networked and distributed setting, named the distributed SAAWET (DSAAWET).
Dynamic Modeling and Control of Antagonistic Variable Stiffness Joint Actuator
Authors: Zhang Ming , Ma Pengfei , Sun Feng , Sun Xingwei , Xu Fangchao , Jin Junjie , Fang Lijin
Published: June 2021

This study aims to develop a novel decoupling method for the independent control of the position and stiffness of a variable stiffness joint actuator (VSJA), which has been proven to be able to vary its stiffness in a larger range than other variable stiffness actuators. Using static analysis and the Jacobian matrix, we obtained the model of the stiffness of the robot joint actuator and dynamics. Based on the hybrid dynamic model of position and stiffness, it is possible to compensate for the torque of the variable stiffness joint actuator (VSJA) to enhance position control. Finally, after describing the actuator prototype, the established compliance control method is verified using simulation and experimental analysis.
Output Feedback Tracking Sliding Mode Control for Systems with State- and Input-Dependent Disturbances
Authors: Yeh Yi-Liang
Published: June 2021

In this paper, output feedback tracking sliding mode control was considered for uncertain multivariable linear systems. The uncertainties included external disturbance, the system state, and control input. A new property of the loop transfer recovery (LTR) observer was first established: the state estimation error of the LTR observer can be made arbitrarily small with respect to state- and input-dependent system uncertainties. Observer-based output feedback tracking sliding mode control using the LTR observer is presented. The proposed sliding mode control approach can maintain the boundedness of the system state and drive the system outputs arbitrarily close to the desired reference outputs; the degree of closeness was determined by a design parameter in the LTR observer. In the proposed approach, the most general and simple observer-based output feedback control formulation was used to achieve global tracking. Simulations with a two-degree-of-freedom (DOF) robotic manipulator application illustrated the claimed properties, and a peaking and chattering reduction technique was demonstrated to protect the actuator.
The Influence of Environmental Factors on Seed Germination of Polygonum perfoliatum L.: Implications for Management
Authors: Farooq Shahid , Onen Huseyin , Tad Sonnur , Ozaslan Cumali , Mahmoud Samy F. , Brestic Marian , Zivcak Marek , Skalicky Milan , El-Shehawi Ahmed M.
Published: June 2021

Polygonum perfoliatum L. is an aggressive vine, currently invading the Black Sea region, Turkey. However, information about the seed germination biology of this species is scanty. The objective of the current study was to determine the seed germination biology of three naturalized populations of this species. Chemical scarification with 98% sulfuric acid for 30 min followed by cold-wet stratification at 4 °C for 4 weeks effectively released seed dormancy in tested populations. Seeds of all populations required a 12 h photoperiod for the highest germination, while germination under continuous dark and light remained similar. The seeds were able to germinate under a wide range of constant (5–40 °C) and alternating temperatures, pH (3–11), osmotic potential (0 to −1.4 MPa) and salinity (0–500 mM NaCl). However, the peak germination was observed under 20 °C constant and 20/15 °C alternating day/night temperature, and pH 6.8. Seeds of all populations were able to withstand 200 mM salinity and −0.6 MPa osmotic potential. Increasing seed burial depth initially stimulated seedling emergence and then a sharp decline was observed for the seeds buried below >2 cm depth. More than 90% of the seeds were unable to emerge when buried >6 cm depth. Polygonum perfoliatum has a large potential for range expansion; therefore, immediate management of the naturalized populations is warranted. This weed species in agricultural fields can be managed by burying the seeds in deeper soil layers (6 cm), while post-emergence management strategies need to be developed for roadside populations.
Variations of Soil Salinity and Cotton Growth under Six-Years Mulched Drip Irrigation
Authors: Li Wenhao , Wang Zhenhua , Zhang Jinzhu , Liu Ningning
Published: June 2021

The lowering of salt content in the field, especially in arid areas, after consecutive application of mulched drip irrigation (MDI) is of vital importance for sustainable cotton plantation. To elucidate the effects of long-term MDI on soil properties and cotton growth, this paper systematically monitored the soil salinity, ion concentrations and the yield of cotton in the field using MDI consecutively for six years in a typical oasis in Xinjiang, China. The results showed that MDI could significantly change salt distribution in the cotton field. During the six years tested, the soil salt content using MDI declined fast at first, and then the decline rate gradually decreased. In the 1st and 2nd year, the average salt content within 0–100 cm soil layer was larger than 20 g kg−1, which belonging to the saline soil. Then the salt content decreased to 10–20 g kg−1 in the 3rd and 4th year, and the cotton field declined to heavily saline soil. After 5 years of MDI, the soil turned to non-salinized. The Cl− and SO42− equivalence ratio (CSER) also decreased with the increase of application years of MDI. Saline-alkaline land developed from chloride-sulphate solonchak (0.2 < CSER < 1) into sulphate solonchak (CSER < 0.2) after 6 years of MDI. The survival rate of the cotton increased from 1.48% (1 year of MDI) to 76.3% (6 years of MDI), and the yield increased from 72.43 kg ha−1 to 4515.48 kg ha−1. When the average CSER, SAR and the soil salinity in 0–140 cm soil layer decreased to 0.60, 0.98 (mol kg−1)0.5 and 6.25 g kg−1, farmers can achieve a balance between income and expenditure. Moreover, when CSER, SAR, and the soil salinity continuously decreased to 0.44, 0.69 (mol kg−1)0.5 and 0.77 g kg−1, the cotton yield will exceed the average production level of cotton in Xinjiang. Under the current irrigation schedule in the oasis irrigation area, the soil salinity and groundwater level after applying MDI could be conducive to cotton growth. However, this situation had also caused a waste of nearly 200 mm of water resources. Therefore, authors suggested that further research on water-saving irrigation systems suitable for different soil conditions should be carried out, and also the differential quota management in production practice should be adopted.
Effect of Graded Substitution of Soybean Meal by Hermetia illucens Larvae Meal on Animal Performance, Apparent Ileal Digestibility, Gut Histology and Microbial Metabolites of Broilers
Authors: Hartinger Kristina , Greinix Julia , Thaler Nathalie , Ebbing Marco Antonio , Yacoubi Nadia , Schedle Karl , Gierus Martin
Published: June 2021

The usage of insects as an alternative protein source for broiler feeds may help to reduce the dependency on soybean meal (SBM) imports. Therefore, the present study aimed to evaluate the replacement of 15 (SL15) or 30% (SL30) of crude protein (CP) from SBM with Hermetia illucens (HI) defatted larvae meal regarding broiler performance, carcass traits, apparent ileal digestibility, intestinal morphology, and microbial metabolites. Concerning the performance, body weight was similar for the control (CON) and SL15, but lower for SL30 during all feeding phases. In addition, average daily feed intake was higher in SL15 and SL30 compared to CON in the starter phase, but this effect vanished during grower and finisher phase. The apparent ileal digestibility decreased for CP and some amino acids with increasing HI larvae meal in the diet. No or marginal alterations were observed for the intestinal morphometry as well as cecal microbial metabolites. In conclusion, partial replacement of 15% SBM CP with HI larvae meal in broiler diets without impairing animal performance or health seems possible. The growth suppression with 30% CP substitution may be caused by reduced apparent ileal digestibility but could not be clearly associated with adverse effects of hindgut fermentation or altered gut morphology.
SARS-Cov-2 Natural Infection in a Symptomatic Cat: Diagnostic, Clinical and Medical Management in a One Health Vision
Authors: Natale Alda , Mazzotta Elisa , Mason Nicoletta , Ceglie Letizia , Mion Monica , Stefani Annalisa , Fincato Alice , Bonfante Francesco , Bortolami Alessio , Monne Isabella , Bellinati Laura , Guadagno Carmine , Quaranta Erika , Pastori Ambra , Terregino Calogero
Published: June 2021

Despite the reported increase in SARS-CoV-2-infected pets, the description of the clinical features from natural infection and the medical follow up in symptomatic pets is still not sufficiently documented. This study reports the case of an indoor cat that displayed respiratory signs and a gastrointestinal syndrome, following the COVID-19 diagnosis of his owners. Thoracic radiographies were suggestive of bronchial pneumonia, while blood tests were indicative of a mild inflammatory process. Nasal and oropharyngeal swabs tested positive through RT-qPCR assays targeting SARS-CoV-2 genes 14 days after his owners tested positive for the virus. Nasal swabs persisted to be RT-qPCR positive after 31 days. Serology confirmed the presence of antibodies through ELISA, electrochemiluminescence analysis and plaque reduction neutralization test, recording a high antibody titre after 31 days. The cat improved after medical treatment and clinically recovered. This study suggests that exposure to SARS-CoV-2 could lead to a natural infection with bronchial pneumonia in cats along with a possible prolonged persistence of SARS-CoV-2 RNA in the upper airways, albeit at a low level. The cat developed neutralizing antibodies, reaching a high titre after 31 days. Further descriptions of SARS-CoV-2 naturally infected pets, their medical management and diagnostic findings would be useful to enhance knowledge about COVID-19 in susceptible animals.
Assessment of a Rapid Semi-Quantitative Immunochromatographic Test for the Evaluation of Transfer of Passive Immunity in Calves
Authors: Delhez Pauline , Meurette Elise , Knapp Emilie , Theron Léonard , Daube Georges , Rao Anne-Sophie
Published: June 2021

Calves are born agammaglobulinemic and they rely on transfer of passive immunity (TPI) through ingestion of colostrum from the dam. Ensuring the effectiveness of TPI through blood serum immunoglobulins (IgG) quantification is of critical importance for the prevention of calf diseases. The main objective of this study was to assess the performance of a novel on-farm immunochromatographic quick assay (SmartStripsTM, Bio-X Diagnostics, Rochefort, Belgium) compared to the ELISA reference method to directly measure serum IgG concentration and assess TPI status in beef and dairy calves. Additional comparison was made with the commonly used Brix refractometer. Jugular blood samples were collected from beef (n = 71) and dairy (n = 26) calves in Belgium within 7 days post-birth. Quantitative (Pearson correlation coefficients, Bland-Altman plots) and qualitative (diagnostic test characteristics, weighted kappa for classification into 4 categories of TPI) analyses were performed to evaluate the performances of the quick test and the refractometer compared to ELISA. The quick test showed a correlation of 0.83 and a classification agreement (weighted kappa) of 0.79 with the reference method (average values for two types of blood anticoagulants). Performances were better for low IgG concentrations and the assessment of poor TPI status and they outperformed those of the Brix refractometer. Results suggested that the immunochromatographic quick test can be considered as a suitable on-farm method for direct serum IgG measurement and the assessment of TPI status in calves, contributing to timely interventions in the management of calves with inadequate TPI.
The Effects of a Fermented Rapeseed or/and Soybean Meal Additive on Antioxidant Parameters in the Blood and Tissues of Piglets
Authors: Czech Anna , Sembratowicz Iwona , Kiesz Martyna
Published: June 2021

The aim of the study was to assess the effect of fermented soybean meal (FSBM) and/or rapeseed meal (FRSM) on the redox status of blood and tissues in piglets. The experiment was conducted on 150 28-day-old weaned piglets divided into five groups. Piglets in the control group received standard diets with soybean meal. Animals in the experimental groups received diets in which a portion of the soybean meal was replaced with FRSM and/or FSBM: group FR—8% FRSM; group FR/FS—6% FRSM and 2% FSBM; group FS/FR—2% FRSM and 6% FSBM; and group FS—8% FSBM. Group FR/FS showed an increase in total antioxidant potential of plasma (FRAP) and low-molecular-weight antioxidants, i.e., vitamin C, urea, uric acid, and albumin, as well as an increase in catalase activity. Blood levels of lipid hydroperoxides (LOOH) and malonyl dialdehyde (MDA) were decreased. A reduction in lipid peroxidation due to the use of FR/FS was also indicated by a decrease in liver MDA and jejunum wall LOOH levels. Increases in superoxide dismutase (SOD) and catalase (CAT) activity and vitamin C levels in these tissues were also noted. The results of the study indicate that the inclusion of fermented rapeseed meal in the diet (6%) in combination with soybean meal (2%), improved the redox status of the weaners.
Prediction of Thiol Group Changes in Minced Raw and Cooked Chicken Meat with Plant Extracts—Kinetic and Neural Network Approaches
Authors: Kaczmarek Anna , Muzolf-Panek Małgorzata
Published: June 2021

The aim of the study was to develop predictive models of thiol group (SH) level changes in minced raw and heat-treated chicken meat enriched with selected plant extracts (allspice, basil, bay leaf, black seed, cardamom, caraway, cloves, garlic, nutmeg, onion, oregano, rosemary, and thyme) during storage at different temperatures. Meat samples with extract addition were stored under various temperatures (4, 8, 12, 16, and 20 °C). SH changes were measured spectrophotometrically using Ellman’s reagent. Samples stored at 12 °C were used as the external validation dataset. SH content decreased with storage time and temperature. The dependence of SH changes on temperature was adequately modeled by the Arrhenius equation with average high R2 coefficients for raw meat (R2 = 0.951) and heat-treated meat (R2 = 0.968). Kinetic models and artificial neural networks (ANNs) were used to build the predictive models of thiol group decay during meat storage. The obtained results demonstrate that both kinetic Arrhenius (R2 = 0.853 and 0.872 for raw and cooked meat, respectively) and ANN (R2 = 0.803) models can predict thiol group changes in raw and cooked ground chicken meat during storage.
Effects of Different β-Lactam Antibiotics on Indirect Tomato (Solanum lycopersicum L.) Shoot Organogenesis and Agrobacterium tumefaciens Growth Inhibition In Vitro
Authors: Varlamova Nataliya V. , Dolgikh Yuliya I. , Blinkov Andrey O. , Baranova Ekaterina N. , Khaliluev Marat R.
Published: June 2021

A β-lactams that act by inhibiting the bacterial cell wall biosynthesis are one of the most common classes of antibiotics applied to suppress the growth of latent bacterial infection associated with the plant tissue culture, as well as in the Agrobacterium-mediated transformation techniques. Plant sensitivity to antibiotics usually is species-, genotype-, or even tissue-specific and mainly depends on concentrations, growth conditions, and culture system. In the presented article, we estimated a comparative effect of four β-lactam antibiotics (Claforan®, timentin, amoxicillin, and Amoxiclav®) at different concentrations in an agar-solidified Murashige and Skoog (MS) culture medium supplemented with 5 mg L−1 6-benzylaminopurine (6-BA) and 0.1 mg L−1 indole-3-acetic acid (IAA) on in vitro callus induction and shoot organogenesis from hypocotyl and cotyledon explants of two tomato cultivars (Rekordsmen, Moryana). The role of clavulanic acid in combination with amoxicillin (Amoxiclav®) in the shoot organogenesis frequency and number of shoots per explant has been demonstrated. Additionally, the growth inhibition of Agrobacterium tumefaciens AGL0 strain according to agar disk-diffusion assay was studied. As a result, both stimulatory (timentin, amoxicillin, and Amoxiclav®) and inhibitory (Claforan®) effects of β-lactam antibiotics on in vitro morphogenetic responses of tomato were noted. It was found that clavulanic acid, which is part of the commercial antibiotic Amoxiclav®, significantly increased the shoot regeneration frequency from cotyledon and hypocotyl explants of Rekordsmen tomato cultivar. Possible reasons for the stimulating effect of clavulanic acid on the induction of shoot organogenesis are discussed. According to agar disk-diffusion assay, the maximum diameter of growth inhibition zones (43.9 mm) was identified using 200 mg L−1 timentin. The in vitro antibacterial activity of tested β-lactam antibiotics was arranged in the following order: timentin > Claforan® > amoxicillin ≥ Amoxiclav®. Thus, to suppress the growth of internal and latent bacterial infection of tomato plant tissue culture, as well as for transformation of Moryana and Rekordsmen cultivars by A. tumefaciens strain AGL0, we recommend adding of 100–200 mg L−1 timentin or 400–800 mg L−1 Amoxiclav® to the shoot induction medium.
Phytochemical Profile and Microbiological Activity of Some Plants Belonging to the Fabaceae Family
Authors: Obistioiu Diana , Cocan Ileana , Tîrziu Emil , Herman Viorel , Negrea Monica , Cucerzan Alexandra , Neacsu Alina-Georgeta , Cozma Antoanela Lena , Nichita Ileana , Hulea Anca , Radulov Isidora , Alexa Ersilia
Published: June 2021

This study aimed to investigate the chemical composition and the activity against Staphylococcus aureus (S. aureus) (ATCC 25923), Streptococcus pyogenes (S. pyogenes) (ATCC 19615), Escherichia coli (E. coli) (ATCC 25922), Pseudomonas aeruginosa (P. aeruginosa) (ATCC 27853), Shigella flexneri (S. flexneri) (ATCC 12022), Salmonella typhimurium (S. typhimurium) (ATCC 14028), Haemophillus influenzae (H. influenza) type B (ATCC 10211) and two fungal strains: Candida albicans (C. albicans) (ATCC 10231) and Candida parapsilopsis (C. parapsilopsis) (ATCC 22019) of the extracts obtained from Melilotus officinalis (MO), Coronilla varia (CV); Ononis spinosa (OS) and Robinia pseudoacacia (RP) (Fabaceae), and to identify the chemical compounds responsible for the antimicrobial effect against the tested strains. The extracts were obtained by conventional hydroalcoholic extraction and analyzed in terms of total polyphenols using the spectrophotometric method and by liquid chromatography (LC). The results have shown that the highest polyphenols content was recorded in the RP sample (16.21 mg gallic acid equivalent GAE/g), followed by the CV (15.06 mg GAE/g), the OS (13.17 mg GAE/g), the lowest value being recorded for the MO sample (11.94 mg GAE/g). The antimicrobial testing of plant extracts was carried out using the microdilution method. The most sensitive strains identified were: E. coli, S. typhimurium, P. aeruginosa and S. pyogenes, while protocatechuic acid, gallic acid, caffeic acid, quercetin, rutin, and kaempferol were identified as the chemical compounds responsible for the antibacterial effect. The analysis of the correlation between the chemical composition and the antimicrobial effect proved a moderate (r > 0.5) positive correlation between rosmarinic acid and S. pyogenes (r = 0.526), rosmarinic acid and S. typhimurium (r = 0.568), quercetin and C. albicans (r = 0.553), quercetin and S. pyogenes (r = 0.605). Therefore, it suggested possible antimicrobial activity generated by these chemical components. The results recommend the Fabaceae plants as promising candidates for further research to develop novel natural antimicrobial drugs.
Polyphenols and IUGR Pregnancies: Effects of the Antioxidant Hydroxytyrosol on Brain Neurochemistry and Development in a Porcine Model
Authors: Yeste Natalia , Valent Daniel , Arroyo Laura , Vázquez-Gómez Marta , García-Contreras Consolación , Pumarola Martí , González-Bulnes Antonio , Bassols Anna
Published: June 2021

Supplementation of a mother’s diet with antioxidants, such as hydroxytyrosol (HTX), has been proposed to ameliorate the adverse phenotypes of fetuses at risk of intrauterine growth restriction. In the present study, sows were treated daily with or without 1.5 mg of HTX per kilogram of feed from day 35 of pregnancy (at 30% of total gestational period), and individuals were sampled at three different ages: 100-day-old fetuses and 1-month- and 6-month-old piglets. After euthanasia, the brain was removed and the hippocampus, amygdala, and prefrontal cortex were dissected. The profile of the catecholaminergic and serotoninergic neurotransmitters (NTs) was characterized and an immunohistochemical study of the hippocampus was performed. The results indicated that maternal supplementation with HTX during pregnancy affected the NT profile in a brain-area-dependant mode and it modified the process of neuron differentiation in the hippocampal CA1 and GD areas, indicating that cell differentiation occurred more rapidly in the HTX group. These effects were specific to the fetal period, concomitantly with HTX maternal supplementation, since no major differences remained between the control and treated groups in 1-month- and 6-month-old pigs.
Profiling Dopamine-Induced Oxidized Proteoforms of β-synuclein by Top-Down Mass Spectrometry
Authors: Luise Arianna , De Cecco Elena , Ponzini Erika , Sollazzo Martina , Mauri PierLuigi , Sobott Frank , Legname Giuseppe , Grandori Rita , Santambrogio Carlo
Published: June 2021

The formation of multiple proteoforms by post-translational modifications (PTMs) enables a single protein to acquire distinct functional roles in its biological context. Oxidation of methionine residues (Met) is a common PTM, involved in physiological (e.g., signaling) and pathological (e.g., oxidative stress) states. This PTM typically maps at multiple protein sites, generating a heterogeneous population of proteoforms with specific biophysical and biochemical properties. The identification and quantitation of the variety of oxidized proteoforms originated under a given condition is required to assess the exact molecular nature of the species responsible for the process under investigation. In this work, the binding and oxidation of human β-synuclein (BS) by dopamine (DA) has been explored. Native mass spectrometry (MS) has been employed to analyze the interaction of BS with DA. In a second step, top-down fragmentation of the intact protein from denaturing conditions has been performed to identify and quantify the distinct proteoforms generated by DA-induced oxidation. The analysis of isobaric proteoforms is approached by a combination of electron-transfer dissociation (ETD) at each extent of modification, quantitation of methionine-containing fragments and combinatorial analysis of the fragmentation products by multiple linear regression. This procedure represents a promising approach to systematic assessment of proteoforms variety and their relative abundance. The method can be adapted, in principle, to any protein containing any number of methionine residues, allowing for a full structural characterization of the protein oxidation states.