bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing High School Student’s STEM Career Interests Using a Social Cognitive Framework
Authors: Mau Wei-Cheng , Chen Shr-Jya , Lin Chi-Chau
Published: June 2019

This study investigated the psychometric properties of the Chinese version of the STEM Career Interest Survey (STEM-CCIS) with data from 590 high-school students in Taiwan. Measurement models based on Social-Cognitive Career Theory (SCCT) and STEM discipline-specific dimensions (Science, Technology, Engineering, Mathematics) were examined using confirmatory factor analyses. Findings from confirmatory factor analyses indicated that STEM-CCIS possesses adequate reliability and factorial validity, replicating the sound psychometric properties of the original English version of the STEM-CIS. Implications for the use of the STEM-CCIS are discussed.
Joint Power Allocation and Link Selection for Multi-Carrier Buffer Aided Relay Network
Authors: Jabeen Tayyaba , Ali Zain , Khan Wali Ullah , Jameel Furqan , Khan Imran , Sidhu Guftaar Ahmad Sardar , Choi Bong Jun
Published: June 2019

In this paper, we present a joint power allocation and adaptive link selection protocol for an orthogonal frequency division multiplexing (OFDM)-based network consists of one source node i.e., base station (BS), one destination node i.e., (MU) and a buffer aided decode and forward (DF) relay node. Our objective is to maximize the average throughput of the system via power loading over different subcarriers at source and relay nodes. A separate power budget is assumed at each transmitting node to make the system more practical. In order to form our solution more tractable, a decomposition framework is implemented to solve the mixed integer optimization problem. Further, less complex suboptimal approaches have also been presented and simulation results are provided to endorse the efficiency of our designed algorithms.
Analysis of Effective Three-Level Neutral Point Clamped Converter System for the Bipolar LVDC Distribution
Authors: Kim Ju-Yong , Kim Ho-Sung , Baek Ju-Won , Jeong Dong-Keun
Published: June 2019

Low-voltage direct current (LVDC) distribution has attracted attention due to increased DC loads, the popularization of electric vehicles, energy storage systems (ESS), and renewable energy sources such as photovoltaic (PV). This paper studies a ±750 V bipolar DC distribution system and applies a 3-level neutral-point clamped (NPC) AC/DC converter for LVDC distribution. However, the 3-level NPC converter is fundamental in the neutral-point (NP) imbalance problem. This paper discusses the NP balance control method using zero-sequence voltage among various solutions to solve NP imbalance. However, since the zero-sequence voltage for NP balance control is limited, the NP voltage cannot be controlled to be balanced when extreme load differences occur. To maintain microgrid stability with bipolar LVDC distribution, it is necessary to control the NP voltage balance, even in an imbalance of extreme load. In addition, due to the bipolar LVDC distribution, the pole where a short-circuit condition occurs limits the short current until the circuit breaker operates, and a pole without a short-circuit condition must supply a stable voltage. Since the conventional 3-level NPC AC/DC converter alone cannot satisfy both functions, an additional DC/DC converter is proposed, analyzed, and verified. This paper is about a 3-level NPC AC/DC converter system for LVDC distribution. It can be used for the imbalance and short-circuit condition in bipolar LVDC distribution through the prototype of the 300 kW 3-level NPC AC/DC converter system and experimented and verified in various conditions.
Intelligent Control of Converter for Electric Vehicles Charging Station
Authors: Jha Mayank , Blaabjerg Frede , Khan Mohammed Ali , Bharath Kurukuru Varaha Satya , Haque Ahteshamul
Published: June 2019

Electric vehicles (EVs) are envisaged to be the future transportation medium, and demonstrate energy efficiency levels much higher than conventional gasoline or diesel-based vehicles. However, the sustainability of EVs is only justified if the electricity used to charge these EVs is availed from a sustainable source of energy and not from any fossil fuel or carbon generating source. In this paper, the challenges of the EV charging stations are discussed while highlighting the growing use of distributed generators in the modern electrical grid system. The benefits of the adoption of photovoltaic (PV) sources along with battery storage devices are studied. A multiport converter is proposed for integrating the PV, charging docks, and energy storage device (ESD) with the grid system. In order to control the bidirectional flow between the generating sources and the loads, an intelligent energy management system is proposed by adapting particle swarm optimization for efficient switching between the sources. The proposed system is simulated using MATLAB/Simulink environment, and the results depicted fast switching between the sources and less switching time without obstructing the fast charging to the EVs.
An Improved DA-PSO Optimization Approach for Unit Commitment Problem
Authors: Khunkitti Sirote , R. Watson Neville , Chatthaworn Rongrit , Premrudeepreechacharn Suttichai , Siritaratiwat Apirat
Published: June 2019

Solving the Unit Commitment problem is an important step in optimally dispatching the available generation and involves two stages—deciding which generators to commit, and then deciding their power output (economic dispatch). The Unit Commitment problem is a mixed-integer combinational optimization problem that traditional optimization techniques struggle to solve, and metaheuristic techniques are better suited. Dragonfly algorithm (DA) and particle swarm optimization (PSO) are two such metaheuristic techniques, and recently a hybrid (DA-PSO), to make use of the best features of both, has been proposed. The original DA-PSO optimization is unable to solve the Unit Commitment problem because this is a mixed-integer optimization problem. However, this paper proposes a new and improved DA-PSO optimization (referred to as iDA-PSO) for solving the unit commitment and economic dispatch problems. The iDA-PSO employs a sigmoid function to find the optimal on/off status of units, which is the mixed-integer part of obtaining the Unit Commitment problem. To verify the effectiveness of the iDA-PSO approach, it was tested on four different-sized systems (5-unit, 6-unit, 10-unit, and 26-unit systems). The unit commitment, generation schedule, total generation cost, and time were compared with those obtained by other algorithms in the literature. The simulation results show iDA-PSO is a promising technique and is superior to many other algorithms in the literature.
Joint Balanced Routing and Energy Harvesting Strategy for Maximizing Network Lifetime in WSNs
Authors: Yu Chih-Min , Tala’t Mohammad , Chiu Chun-Hao , Huang Chin-Yao
Published: June 2019

Nowadays, wireless sensor networks (WSNs) are becoming increasingly popular due to the wide variety of applications. The network can be utilized to collect and transmit numerous types of messages to a data sink in a many-to-one fashion. The WSNs usually contain sensors with low communication ability and limited battery power, and the battery replacement is difficult in WSNs for large amount embedded nodes, which indicates a balanced routing strategy is essential to be developed for an extensive operation lifecycle. To realize the goal, the research challenges require not only to minimize the energy consumption in each node but also to balance the whole WSNs traffic load. In this article, a Shortest Path Tree with Energy Balance Routing strategy (SPT-EBR) based on a forward awareness factor is proposed. In SPT-EBR, Two methods are presented including the power consumption and the energy harvesting schemes to select the forwarding node according to the awareness factors of link weight. First, the packet forwarding rate factor is considered in the power consumption scheme to update the link weight for the sensors with higher power consumption and mitigate the traffic load of hotspot nodes to achieve the energy balance network. With the assistance of the power consumption scheme, hotspot nodes can be transferred from the irregular location to the same intra-layer from the sink. Based on this feature, the energy harvesting scheme combines both the packet forwarding rate and the power charging rate factors together to update the link weight with a new battery charging rate factor for hotspot nodes. Finally, simulation results validate that both power consumption and energy harvesting schemes in SPT-EBR achieve better energy balance performance and save more charging power than the conventional shortest path algorithm and thus improve the overall network lifecycle.
On Wind Turbine Power Delta Control
Authors: Elorza Iker , Calleja Carlos , Pujana-Arrese Aron
Published: June 2019

One of the major challenges facing wind energy at the moment is its dependence on dispatchable energy sources to match power supply to demand and provide an adequate spinning reserve. There is no fundamental impediment for this to be done with wind energy when wind conditions are such that sufficient wind power is available. It is, in fact, common for wind farms to participate in primary and secondary frequency regulation via droop curves, curtailment, synthetic inertia, proportional de-loading, and delta control. However, although the literature presents several approaches to turbine-level control functions of this sort, it is not trivial to extract from it a readily industrializable set of algorithms. Said extraction, focused on delta control and the addition of our own contributions, is the purpose of this paper, where we propose an extension of popular torque and pitch control algorithms, which allows delta control without the wind speed observers used by other authors.
Fault-Tolerant Control for Actuator Faults of Wind Energy Conversion System
Authors: You Guodong , Xu Tao , Su Honglin , Hou Xiaoxin , Li Jisheng
Published: June 2019

The problem of robust fault-tolerant control for actuators of nonlinear systems with uncertain parameters is studied in this paper. Takagi–Sugeno (T-S) fuzzy model is used to describe the wind energy conversion system (WECS). Fuzzy dedicated observer (FDO) and fuzzy proportional integral observer (FPIO) are established to reconstruct the system state and actuator fault, respectively. Fuzzy Robust Scheduling Fault-Tolerant Controller (FRSFTC) is designed by parallel distributed compensation (PDC) method, so as to realize the purpose of active fault tolerance for actuator faults and ensure the robust stability of the system. The stability of the closed-loop system is proved by Taylor series, Lyapunov function, and Linear Matrix Inequalities (LMIs). Finally, the simulation results verify that the proposed method is feasible and effective applied to WECS with doubly fed induction generators (DFIG).
On Extended General Mittag–Leffler Functions and Certain Inequalities
Authors: Mihai Marcela V. , Awan Muhammad Uzair , Noor Muhammad Aslam , Du Tingsong , Kashuri Artion , Noor Khalida Inayat
Published: June 2019

In this paper, we introduce and investigate generalized fractional integral operators containing the new generalized Mittag–Leffler function of two variables. We establish several new refinements of Hermite–Hadamard-like inequalities via co-ordinated convex functions.
Prediction of Bone Metastasis in Breast Cancer Based on Minimal Driver Gene Set in Gene Dependency Network
Authors: Li Jia-Nuo , Zhong Rui , Zhou Xiong-Hui
Published: June 2019

Bone is the most frequent organ for breast cancer metastasis, and thus it is essential to predict the bone metastasis of breast cancer. In our work, we constructed a gene dependency network based on the hypothesis that the relation between one gene and the risk of bone metastasis might be affected by another gene. Then, based on the structure controllability theory, we mined the driver gene set which can control the whole network in the gene dependency network, and the signature genes were selected from them. Survival analysis showed that the signature could distinguish the bone metastasis risks of cancer patients in the test data set and independent data set. Besides, we used the signature genes to construct a centroid classifier. The results showed that our method is effective and performed better than published methods.
Assessing the Multiple Impacts of Extreme Hurricanes in Southern New England, USA
Authors: Ullman David S. , Ginis Isaac , Huang Wenrui , Nowakowski Catherine , Chen Xuanyu , Stempel Peter
Published: June 2019

The southern New England coast of the United States is particularly vulnerable to land-falling hurricanes because of its east-west orientation. The impact of two major hurricanes on the city of Providence (Rhode Island, USA) during the middle decades of the 20th century spurred the construction of the Fox Point Hurricane Barrier (FPHB) to protect the city from storm surge flooding. Although the Rhode Island/Narragansett Bay area has not experienced a major hurricane for several decades, increased coastal development along with potentially increased hurricane activity associated with climate change motivates an assessment of the impacts of a major hurricane on the region. The ocean/estuary response to an extreme hurricane is simulated using a high-resolution implementation of the ADvanced CIRCulation (ADCIRC) model coupled to the Precipitation-Runoff Modeling System (PRMS). The storm surge response in ADCIRC is first verified with a simulation of a historical hurricane that made landfall in southern New England. The storm surge and the hydrological models are then forced with winds and rainfall from a hypothetical hurricane dubbed “Rhody”, which has many of the characteristics of historical storms that have impacted the region. Rhody makes landfall just west of Narragansett Bay, and after passing north of the Bay, executes a loop to the east and the south before making a second landfall. Results are presented for three versions of Rhody, varying in the maximum wind speed at landfall. The storm surge resulting from the strongest Rhody version (weak Saffir–Simpson category five) during the first landfall exceeds 7 m in height in Providence at the north end of the Bay. This exceeds the height of the FPHB, resulting in flooding in Providence. A simulation including river inflow computed from the runoff model indicates that if the Barrier remains closed and its pumps fail (for example, because of a power outage or equipment failure), severe flooding occurs north of the FPHB due to impoundment of the river inflow. These results show that northern Narragansett Bay could be particularly vulnerable to both storm surge and rainfall-driven flooding, especially if the FPHB suffers a power outage. They also demonstrate that, for wind-driven storm surge alone under present sea level conditions, the FPHB will protect Providence for hurricanes less intense than category five.
The Influence of Olive Orchards Copper-Based Fungicide Use, in Soils and Sediments—The Case of Aetoliko (Etoliko) Lagoon Western Greece
Authors: Avramidis Pavlos , Barouchas Pantelis , Dünwald Thomas , Unkel Ingmar , Panagiotaras Dionisios
Published: June 2019

In this study, soil and aquatic sediments were sampled in the Aetoliko Lagoon and its catchment area, which is exclusively dominated by olive orchards. For the first time in Greece, soil as well as sediments samples of one coherent protected aquatic ecosystem were directly compared. In order to determine the influence that the usage of copper-based fungicides have on the lagoon sediments and on the soils of the surrounding area, twenty five (25) soil samples from different olive orchards that are bordering the water body and ten (10) sediment samples from the bottom of the lagoon were taken. The samples were analyzed for total copper content (total digestion) and extractable copper (diethylenetriaminepenta acetic acid, DTPA, extraction method). Furthermore, soil/sedimentological and geochemical analyses such as pH, grain size, total organic carbon, total sulfur, total nitrogen, and calcium carbonate content were carried out. The results show that the total copper in soils ranges from 58.37 to 671.33 mg kg−1. In addition the DTPA-extractable copper in soils has an average value of 45.00 mg kg−1. The average value of total copper in soils (286.24 mg kg−1) is higher than the threshold value for the Cu concentration (100 mg kg−1) set by the EU countries. Total copper content in the lagoon sediments is lower than in soils and varies between 43.85 mg kg−1 and 71.87 mg kg−1. The DTPA-extractable copper in sediments is in low ranges from 0.14 to 0.60 mg kg−1. On average, the total copper value for the lagoon sediments (55.93 mg kg−1) exceeds the Toxicity Screening Value (25.20 mg kg−1) for Cu in freshwater sediments. From the present study, it is clear that, although the copper in soils of the surrounding lagoon area exceeds the threshold limit for ecological risk, the lagoon sediments are influenced in a smaller degree. Our study can be used as a valuable reference and baseline for future studies on the environmental monitoring of the Aetoliko lagoon, as well as for studies in similar ecosystems.
Degradation Characteristics of Phosphorus in Phytoplankton-Derived Particulate Organic Matter and Its Effects on the Growth of Phosphorus-Deficient Microcystis aeruginosa in Lake Taihu
Authors: Kong Ming , Chao Jianying , Han Wei , Ye Chun , Li Chun-Hua , Tian Wei
Published: June 2019

To illustrate the contribution of phytoplankton-derived particulate organic matter (PPOM) to endogenous phosphorus (P) cycling and its effects on cyanobacteria blooms, PPOM characteristics, the degradation mechanism, and the growth of P-deficient Microcystis aeruginosa were studied in Lake Taihu. Results showed that PPOM is the most important P pool in the water column during cyanobacteria bloom, accounting for more than 80% of the total P (TP) in the water. During PPOM degradation, the particulate orthophosphate (Ortho-P) is the main species of P release from PPOM in the early degradation stage. The variations of polyphosphate (Poly-P) and phosphodiesters (Diester-P) contents were most significant, which were degraded completely within four days and eight days. Cell density and growth rate of M. aeruginosa using PPOM as P source were similar to those growing on Na2HPO4. The above results show that P in PPOM can be converted into available P by degradation, thus promoting the growth of M. aeruginosa. Therefore, the contribution of P release from PPOM degradation needs to be paid attention to in lake eutrophication control in the future.
The Functional and Morphological Changes of the Cervical Intervertebral Disc after Applying Lordotic Curve Controlled Traction: A Double-Blind Randomized Controlled Study
Authors: Lee Chang-Hyung , Heo Sung Jin , Park So Hyun , Jeong Hee Seok , Kim Soo-Yeon
Published: June 2019

The newly developed cervical lordotic curve-controlled traction (C-LCCT) appears to be an ideal method to improve the treatment outcome in patients with cervical intervertebral disc disease. The purpose of this study was to investigate the treatment outcomes of C-LCCT including the functional and morphological changes of the cervical intervertebral disc compared to traditional traction (TT) with a randomized controlled trial design. A total of 40 patients with cervical intervertebral disc disease at the C5/6 level confirmed by magnetic resonance imaging were recruited and assigned to either the C-LCCT group or the TT group. The comprehensive health status changes of the patients were recorded using pain and functional scores (Visual Analogue Scale, Oswestry Disability Index) and morphological changes (cervical lordosis, cervical central canal area) before and after the traction treatment. Both groups showed a significant improvement in pain scores after traction (p < 0.05). The functional score and morphological changes improved significantly after treatment in the C-LCCT group. However, there was no significant improvement in the TT group (p < 0.05). The C-LCCT showed significant pain, functional, and morphological improvement compared to TT. C-LCCT could be effective in improving the treatment outcomes of the traction technique in patients with cervical intervertebral disc disease.
Biomonitoring Study of Deoxynivalenol Exposure in Chinese Inhabitants
Authors: Wang Xiaodan , Liang Jiang , Cao Pei , Zhou Shuang , Wu Aibo , Gao Peng , Xu Haibin , Liu Zhaoping , Gong Yunyun
Published: June 2019

Objective: To investigate the levels of a deoxynivalenol (DON) biomarker in the urine of subjects living in two China provinces with different geographic locations and dietary patterns, and estimate their dietary DON exposures and health risks. Methods: First morning urine samples were collected on three consecutive days from 599 healthy subjects—301 from Henan province and 298 from Sichuan province—to analyze the total DON concentrations (tDON) after β-glucuronidase hydrolysis using a high-performance liquid chromatography tandem mass spectrometry-based method. The consumption of cereal foods in the previous 24 h before each urine collection was recorded using a duplicate diet method. DON exposure levels were estimated based on the urinary tDON concentrations. Results: Total DON were detected in 100% and 92% of the urine samples from Henan and Sichuan, respectively. Mean urinary tDON concentrations were 52.83 ng/mL in Henan subjects and 12.99 ng/mL in Sichuan subjects, respectively. The tDON levels were significantly higher in the urine of Henan subjects than that of the Sichuan subjects (p < 0.001). Urinary tDON levels were significantly different among age groups in both areas (Henan: p < 0.001; Sichuan: p = 0.026) and were highest in adolescents aged 13–17 years, followed by children aged 7–12 years. Based on the DON biomarker and exposure conversion reported by the European Food Safety Authority (EFSA), the mean estimated dietary intakes of DON were 1.82 μg/kg bw/day in Henan subjects and 0.45 μg/kg bw/day in Sichuan subjects. A total of 56% of Henan subjects and 12% of Sichuan subjects were estimated to exceed the PMTDI of 1 μg/kg bw/day. Consistent with urinary tDON levels, the highest estimated dietary DON intakes were also in children and adolescents aged 7–17 years. For all kinds of wheat-based foods except dumplings, the consumptions were significantly higher in Henan than those in Sichuan. The mean consumption of steamed buns was 8.4-fold higher in Henan (70.67 g/d) than that in Sichuan (8.45 g/d). The mean consumption of noodles in Henan (273.91 g/d) was 3.6-fold higher than that in Sichuan (75.87 g/d). Conclusions: The levels of urinary DON biomarker and the estimated dietary DON intakes in Henan province were high and concerning, especially for children and adolescents. The overall exposure level of Sichuan inhabitants was low.
Water-Use Characteristics and Physiological Response of Moso Bamboo to Flash Droughts
Authors: Zhang Minxia , Chen Shulin , Jiang Hong , Lin Yong , Zhang Jinmeng , Song Xinzhang , Zhou Guomo
Published: June 2019

Frequent flash droughts can rapidly lead to water shortage, which affects the stability of ecosystems. This study determines the water-use characteristics and physiological mechanisms underlying Moso bamboo response to flash-drought events, and estimates changes to water budgets caused by extreme drought. We analyzed the variability in forest canopy transpiration versus precipitation from 2011–2013. Evapotranspiration reached 730 mm during flash drought years. When the vapor pressure deficit > 2 kPa and evapotranspiration > 4.27 mm·day−1, evapotranspiration was mainly controlled through stomatal opening and closing to reduce water loss. However, water exchange mainly occurred in the upper 0–50 cm of the soil. When soil volumetric water content of 50 cm was lower than 0.17 m3·m−3, physiological dehydration occurred in Moso bamboo to reduce transpiration by defoliation, which leads to water-use efficiency decrease. When mean stand density was <3500 trees·ha−1, the bamboo forest can safely survive the flash drought. Therefore, we recommend thinning Moso bamboo as a management strategy to reduce transpiration in response to future extreme drought events. Additionally, the response function of soil volumetric water content should be used to better simulate evapotranspiration, especially when soil water is limited.
Confining a Protein-Containing Water Nanodroplet inside Silica Nanochannels
Authors: Giussani Lara , Tabacchi Gloria , Coluccia Salvatore , Fois Ettore
Published: June 2019

Incorporation of biological systems in water nanodroplets has recently emerged as a new frontier to investigate structural changes of biomolecules, with perspective applications in ultra-fast drug delivery. We report on the molecular dynamics of the digestive protein Pepsin subjected to a double confinement. The double confinement stemmed from embedding the protein inside a water nanodroplet, which in turn was caged in a nanochannel mimicking the mesoporous silica SBA-15. The nano-bio-droplet, whose size fits with the pore diameter, behaved differently depending on the protonation state of the pore surface silanols. Neutral channel sections allowed for the droplet to flow, while deprotonated sections acted as anchoring piers for the droplet. Inside the droplet, the protein, not directly bonded to the surface, showed a behavior similar to that reported for bulk water solutions, indicating that double confinement should not alter its catalytic activity. Our results suggest that nanobiodroplets, recently fabricated in volatile environments, can be encapsulated and stored in mesoporous silicas.
Multifaceted Role of the Placental Growth Factor (PlGF) in the Antitumor Immune Response and Cancer Progression
Authors: Albonici Loredana , Giganti Maria Gabriella , Modesti Andrea , Manzari Vittorio , Bei Roberto
Published: June 2019

The sharing of molecules function that affects both tumor growth and neoangiogenesis with cells of the immune system creates a mutual interplay that impairs the host’s immune response against tumor progression. Increasing evidence shows that tumors are able to create an immunosuppressive microenvironment by recruiting specific immune cells. Moreover, molecules produced by tumor and inflammatory cells in the tumor microenvironment create an immunosuppressive milieu able to inhibit the development of an efficient immune response against cancer cells and thus fostering tumor growth and progression. In addition, the immunoediting could select cancer cells that are less immunogenic or more resistant to lysis. In this review, we summarize recent findings regarding the immunomodulatory effects and cancer progression of the angiogenic growth factor namely placental growth factor (PlGF) and address the biological complex effects of this cytokine. Different pathways of the innate and adaptive immune response in which, directly or indirectly, PlGF is involved in promoting tumor immune escape and metastasis will be described. PlGF is important for building up vascular structures and functions. Although PlGF effects on vascular and tumor growth have been widely summarized, its functions in modulating the immune intra-tumoral microenvironment have been less highlighted. In agreement with PlGF functions, different antitumor strategies can be envisioned.
Graphene- and Graphene Oxide-Based Nanocomposite Platforms for Electrochemical Biosensing Applications
Authors: Thangamuthu Madasamy , Hsieh Kuan Yu , Kumar Priyank V. , Chen Guan-Yu
Published: June 2019

Graphene and its derivatives such as graphene oxide (GO) and reduced GO (rGO) offer excellent electrical, mechanical and electrochemical properties. Further, due to the presence of high surface area, and a rich oxygen and defect framework, they are able to form nanocomposites with metal/semiconductor nanoparticles, metal oxides, quantum dots and polymers. Such nanocomposites are becoming increasingly useful as electrochemical biosensing platforms. In this review, we present a brief introduction on the aforementioned graphene derivatives, and discuss their synthetic strategies and structure–property relationships important for biosensing. We then highlight different nanocomposite platforms that have been developed for electrochemical biosensing, introducing enzymatic biosensors, followed by non-enzymatic biosensors and immunosensors. Additionally, we briefly discuss their role in the emerging field of biomedical cell capture. Finally, a brief outlook on these topics is presented.
Multidisciplinary Challenges in Mastocytosis and How to Address with Personalized Medicine Approaches
Authors: Valent Peter , Akin Cem , Gleixner Karoline V. , Sperr Wolfgang R. , Reiter Andreas , Arock Michel , Triggiani Massimo
Published: June 2019

Mastocytosis is a hematopoietic neoplasm defined by abnormal expansion and focal accumulation of clonal tissue mast cells in various organ-systems. The disease exhibits a complex pathology and an equally complex clinical behavior. The classification of the World Health Organization (WHO) divides mastocytosis into cutaneous forms, systemic variants, and localized mast cell tumors. In >80% of patients with systemic mastocytosis (SM), a somatic point mutation in KIT at codon 816 is found. Whereas patients with indolent forms of the disease have a normal or near-normal life expectancy, patients with advanced mast cell neoplasms, including aggressive SM and mast cell leukemia, have a poor prognosis with short survival times. In a majority of these patients, multiple somatic mutations and/or an associated hematologic neoplasm, such as a myeloid leukemia, may be detected. Independent of the category of mastocytosis and the serum tryptase level, patients may suffer from mediator-related symptoms and/or osteopathy. Depending on the presence of co-morbidities, the symptomatology in such patients may be mild, severe or even life-threatening. Most relevant co-morbidities in such patients are IgE-dependent allergies, psychiatric, psychological or mental problems, and vitamin D deficiency. The diagnosis and management of mastocytosis is an emerging challenge in clinical practice and requires vast knowledge, a multidisciplinary approach, and personalized medicine procedures. In this article, the current knowledge about mastocytosis is reviewed with special emphasis on the multidisciplinary aspects of the disease and related challenges in daily practice.
Synthesis of “Dahlia-Like” Hydrophilic Fluorescent Carbon Nanohorn as a Bio-Imaging PROBE
Authors: Parasuraman Perumalswamy Sekar , Parasuraman Vijaya Rohini , Anbazhagan Rajeshkumar , Tsai Hsieh-Chih , Lai Juin-Yih
Published: June 2019

Carbon nanohorns (CNH) were synthesized by a simple conventional hydrothermal method in this study. The CNHs were prepared by the chemical oxidation from the carbonation of Nafion (catalyst) with heparin (carbon resource). The formation of CNH involved two major steps, as described followed. First, the formation of carbon nanorice (CNR) was achieved by carbonation and self-assembly of heparin inside the Nafion structure. Second, the further oxidation of CNR resulted the heterogeneous and porous micelle domains showed at the outer layer of the CNR particles. These porous domains exhibited hydrophobic carbon and resulted self-assembly of the CNR to form the structure of CNHs. The resulting CNHs aggregated into a “dahlia-like” morphology with fluorescence in a diameter of 50–200 nm. The “dahlia-like” CNH showed better fluorescence (450nm) than CNR particles because of the presence of more structural defect. These findings suggest that the hydrophilic fluorescent carbon nanohorns (HFCNHs) synthesized in this study have the potential to be used for in vitro bio-imaging
HNF4α and CDX2 Regulate Intestinal YAP1 Promoter Activity
Authors: Larsen Sylvester , Davidsen Johanne , Dahlgaard Katja , Pedersen Ole B. , Troelsen Jesper T.
Published: June 2019

The Hippo pathway is important for tissue homeostasis, regulation of organ size and growth in most tissues. The co-transcription factor yes-associated protein 1 (YAP1) serves as a main downstream effector of the Hippo pathway and its dysregulation increases cancer development and blocks colonic tissue repair. Nevertheless, little is known about the transcriptional regulation of YAP1 in intestinal cells. The aim of this study to identify gene control regions in the YAP1 gene and transcription factors important for intestinal expression. Bioinformatic analysis of caudal type homeobox 2 (CDX2) and hepatocyte nuclear factor 4 alpha (HNF4α) chromatin immunoprecipitated DNA from differentiated Caco-2 cells revealed potential intragenic enhancers in the YAP1 gene. Transfection of luciferase-expressing YAP1 promoter-reporter constructs containing the potential enhancer regions validated one potent enhancer of the YAP1 promoter activity in Caco-2 and T84 cells. Two potential CDX2 and one HNF4α binding sites were identified in the enhancer by in silico transcription factor binding site analysis and protein-DNA binding was confirmed in vitro using electrophoretic mobility shift assay. It was found by chromatin immunoprecipitation experiments that CDX2 and HNF4α bind to the YAP1 enhancer in Caco-2 cells. These results reveal a previously unknown enhancer of the YAP1 promoter activity in the YAP1 gene, with importance for high expression levels in intestinal epithelial cells. Additionally, CDX2 and HNF4α binding are important for the YAP1 enhancer activity in intestinal epithelial cells.
Biocompatible and Antimicrobial Electrospun Membranes Based on Nanocomposites of Chitosan/Poly (Vinyl Alcohol)/Graphene Oxide
Authors: Tamayo Marín Julián Andrés , Londoño Sebastián Ruiz , Delgado Johannes , Navia Porras Diana Paola , Valencia Zapata Mayra Eliana , Mina Hernandez José Herminsul , Valencia Carlos Humberto , Grande Tovar Carlos David
Published: June 2019

Tissue engineering is gaining attention rapidly to replace and repair defective tissues in the human body after illnesses and accidents in different organs. Electrospun nanofiber scaffolds have emerged as a potential alternative for cell regeneration and organ replacement. In this paper, porous membranes, based on nanofibrous chitosan (CS), polyvinyl alcohol (PVA), and graphene oxide (GO), were obtained via electrospinning methodology. Three different formulations were obtained varying GO content, being characterized by Fourier Transform Infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS). In vitro tests were carried out, consisting of hydrolytic degradation inside simulated biological fluid (SBF), and in vivo tests were carried out, where the material was implanted in Wistar rats’ subcutaneous tissue to determine its biocompatibility. The antibacterial activity was tested against Gram-positive bacteria Bacillus cereus and Staphylococcus aureus, and against Gram-negative Salmonella enterica and Escherichia coli, by contact of the electrospun nanofiber scaffolds above inoculum bacterial in Müeller Hinton agar with good inhibition only for scaffolds with the higher GO content (1.0%). The results confirmed good biocompatibility of the nanofibrous scaffolds after in vivo tests in Wistar rats, which evidences its high potential in applications of tissue regeneration.
Genetic Analysis for Fruit Phenolics Content, Flesh Color, and Browning Related Traits in Eggplant (Solanum melongena)
Authors: Kaushik Prashant
Published: June 2019

Eggplant varieties rich in bioactive chlorogenic acid along with less browning are preferred by consumers. Therefore, genetics of fruit phenolics, fruit flesh colour, and browning related traits were studied in the genotypes of eggplant, comprising of nine cultivated varieties and one accession of eggplant‘s primary genepool wild relative Solanum insanum (INS2). These accessions were genotyped based on the 7335 polymorphic single-nucleotide polymorphisms (SNP) markers. After that, genotypes were crossed in half diallel fashion to produce 45 hybrids. The INS2 displayed the highest values for the total phenolics and chlorogenic acid content (CGA). For all of the biochemical traits studied, significant values of general and specific combining ability (GCA and SCA) effects were determined. The baker ratio estimates were high (>0.75) for all of the traits. Highly significant and positive heterosis (%) was determined for the dry matter, total phenolics, CGA, and area (%) of CGA content. The phenolics content of the fruit (total phenolics and CGA) was not significantly correlated with flesh colour and browning related traits. However, when the path coefficient analysis was performed considering the CGA as a dependent variable, it was determined that the flesh colour related traits most considerably affected the CGA. The genetic distance showed a diminutive correlation with the hybrid means, heterosis, and SCA values. Overall, this study provides important information regarding the underlying genetics of important biochemical traits of eggplant fruit.
Nuclear Phosphoinositides: Their Regulation and Roles in Nuclear Functions
Authors: Fiume R. , Faenza I. , Sheth B. , Poli A. , Vidalle M.C. , Mazzetti C. , Abdul S.H. , Campagnoli F. , Fabbrini M. , Kimber S.T. , Mariani G.A. , Xian J. , Marvi M.V. , Mongiorgi S. , Shah Z. , Divecha N.
Published: June 2019

Polyphosphoinositides (PPIns) are a family of seven lipid messengers that regulate a vast array of signalling pathways to control cell proliferation, migration, survival and differentiation. PPIns are differentially present in various sub-cellular compartments and, through the recruitment and regulation of specific proteins, are key regulators of compartment identity and function. Phosphoinositides and the enzymes that synthesise and degrade them are also present in the nuclear membrane and in nuclear membraneless compartments such as nuclear speckles. Here we discuss how PPIns in the nucleus are modulated in response to external cues and how they function to control downstream signalling. Finally we suggest a role for nuclear PPIns in liquid phase separations that are involved in the formation of membraneless compartments within the nucleus.
Biphasic Effect of Sildenafil on Energy Sensing is Mediated by Phosphodiesterases 2 and 3 in Adipocytes and Hepatocytes
Authors: Banerjee Jheelam , Bruckbauer Antje , Thorpe Teresa , Zemel Michael B.
Published: June 2019

Sirt1 (Sirtuin 1), AMPK (AMP-activated protein kinase), and eNOS (endothelial nitric oxide synthase) modulate hepatic energy metabolism and inflammation and play a major role in the development of NASH. Cyclic nucleotide phosphodiesterases (PDEs) play an important role in signal transduction by modulating intracellular levels of cyclic nucleotides. We previously found the PDE5 inhibitor sildenafil to synergize with leucine and leucine-metformin combinations in preclinical studies of NASH and obesity. However, efficacy is diminished at higher sildenafil concentrations. Herein, we have successfully modeled the U-shaped sildenafil dose-response in vitro and utilized this model to assess potential mechanisms of this dose-response relationship. Adipocytes and liver cells were treated with leucine (0.5 mM) and different concentrations of sildenafil (1 nM to 100 µM). cAMP, cGMP, and P-AMPK protein expression were used to demonstrate the biphasic response for increasing concentrations of sildenafil. The reversal with higher sildenafil levels was blunted by PDE2 inhibition. These data indicate that sildenafil-mediated increases in cGMP inhibits PDE3 at lower concentrations, which increases cAMP. However, further increases in cGMP from higher sildenafil concentrations activate PDE2 and consequently decrease cAMP, which demonstrates crosstalk between cAMP and cGMP via PDE2, PDE3, and PDE5. These changes in cAMP concentration are further reflected in downstream effects, including AMPK activation.
Cucumber Mildew Resistance Locus O Interacts with Calmodulin and Regulates Plant Cell Death Associated with Plant Immunity
Authors: Yu Guangchao , Wang Xiangyu , Chen Qiumin , Cui Na , Yu Yang , Fan Haiyan
Published: June 2019

Pathogen-induced cell death is closely related to plant disease susceptibility and resistance. The cucumber (Cucumis sativus L.) mildew resistance locus O (CsMLO1) and calmodulin (CsCaM3) genes, as molecular components, are linked to nonhost resistance and hypersensitive cell death. In this study, we demonstrate that CsMLO1 interacts with CsCaM3 via yeast two-hybrid, firefly luciferase (LUC) complementation and bimolecular fluorescence complementation (BiFC) experiments. A subcellular localization analysis of green fluorescent protein (GFP) fusion reveals that CsCaM3 is transferred from the cytoplasm to the plasma membrane in Nicotiana benthamiana, and CsCaM3 green fluorescence is significantly attenuated via the coexpression of CsMLO1 and CsCaM3. CsMLO1 negatively regulates CsCaM3 expression in transiently transformed cucumbers, and hypersensitive cell death is disrupted by CsCaM3 and/or CsMLO1 expression under Corynespora cassiicola infection. Additionally, CsMLO1 silencing significantly enhances the expression of reactive oxygen species (ROS)-related genes (CsPO1, CsRbohD, and CsRbohF), defense marker genes (CsPR1 and CsPR3) and callose deposition-related gene (CsGSL) in infected cucumbers. These results suggest that the interaction of CsMLO1 with CsCaM3 may act as a cell death regulator associated with plant immunity and disease.
Amelioration of Behavioral Impairments and Neuropathology by Antiepileptic Drug Topiramate in a Transgenic Alzheimer’s Disease Model Mice, APP/PS1
Authors: Owona Brice Ayissi , Zug Caroline , Schluesener Hermann J. , Zhang Zhi-Yuan
Published: June 2019

Alzheimer’s disease (AD) is a neurodegenerative disease that is the main cause of dementia in the elderly. The aggregation of β-amyloid peptides is one of the characterizing pathological changes of AD. Topiramate is an antiepileptic drug, which in addition, is used in the treatment of many neuropsychiatric disorders. In this study, the therapeutic effects of topiramate were investigated in a transgenic mouse model of cerebral amyloidosis (APP/PS1 mice). Before, during, and after topiramate treatment, behavioral tests were performed. Following a treatment period of 21 days, topiramate significantly ameliorated deficits in nest-constructing capability as well as in social interaction. Thereafter, brain sections of mice were analyzed, and a significant attenuation of microglial activation as well as β-amyloid deposition was observed in sections from topiramate-treated APP/PS1 mice. Therefore, topiramate could be considered as a promising drug in the treatment of human AD.
Optimal Control of Virus Spread under Different Conditions of Resources Limitations
Authors: Di Giamberardino Paolo , Iacoviello Daniela
Published: June 2019

The paper addresses the problem of human virus spread reduction when the resources for the control actions are somehow limited. This kind of problem can be successfully solved in the framework of the optimal control theory, where the best solution, which minimizes a cost function while satisfying input constraints, can be provided. The problem is formulated in this contest for the case of the HIV/AIDS virus, making use of a model that considers two classes of susceptible subjects, the wise people and the people with incautious behaviours, and three classes of infected, the ones still not aware of their status, the pre-AIDS patients and the AIDS ones; the control actions are represented by an information campaign, to reduce the category of subjects with unwise behaviour, a test campaign, to reduce the number of subjects not aware of having the virus, and the medication on patients with a positive diagnosis. The cost function considered aims at reducing patients with positive diagnosis using as less resources as possible. Four different types of resources bounds are considered, divided into two classes: limitations on the instantaneous control and fixed total budgets. The optimal solutions are numerically computed, and the results of simulations performed are illustrated and compared to put in evidence the different behaviours of the control actions.
Endophytic Beauveria bassiana in Foliar-Treated Citrus limon Plants Acting as a Growth Suppressor to Three Successive Generations of Diaphorina citri Kuwayama (Hemiptera: Liviidae)
Authors: Bamisile Bamisope Steve , Dash Chandra Kanta , Akutse Komivi Senyo , Qasim Muhammad , Ramos Aguila Luis Carlos , Wang Fangfei , Keppanan Ravindran , Wang Liande
Published: June 2019

Entomopathogenic fungi are commonly applied as inundative sprays to protect plants against insect pests. Their artificial establishment as fungal endophytes to provide other benefits to the host plants aside mere protection against the primary pests has also been widely demonstrated. In the present study, two fungal strains of Beauveria bassiana and one strain of Isaria fumosorosea were assessed in a pathogenicity test against adults of Asian citrus psyllid (Diaphorina citri) and found to induce 50% reduction in the survival rate of D. citri adults within 5 days of exposure. The ability of the three fungal strains to endophytically colonize Citrus limon, the impact on plant growth and the effects of systemic colonization on 3 successive generations of D. citri feeding on colonized plants was evaluated. Citrus seedlings at 4 months post-planting were inoculated with each of the fungal strains via foliar spraying. Both strains of B. bassiana successfully colonized the seedlings. One of the B. bassiana strains (BB Fafu-13) was sustained up to 12 weeks in the colonized seedlings, whereas the other B. bassiana strain (BB Fafu-16) was only recovered up to 8 weeks post-inoculation. Isaria fumosorosea (IF Fafu-1) failed to colonize the plant. Both strains of B. bassiana induced significant improvement in plant height and flush production in endophytically colonized seedlings. In addition, endophytic B. bassiana caused 10–15% D. citri adult mortality within 7 days of exposure. Female D. citri feeding on B. bassiana challenged plants laid fewer eggs as compared to those feeding on endophyte-free seedlings, while reduction in adult emergence was recorded on B. bassiana treated plants. With this study, we present the first evidence of B. bassiana artificial establishment as fungal endophyte in citrus plants and its negative effects on D. citri.
Sticky Trap Design Considerations for Entrapping Bed Bugs
Authors: Hottel Benjamin A. , Pereira Roberto M. , Gezan Salvador A. , Koehler Philip G.
Published: June 2019

Little evidence has been presented on the usefulness of sticky traps for monitoring bed bugs, Cimex lectularius. We examined how the surface roughness around the adhesive of a sticky trap affects both bed bug behavior and adhesive entrapment. In the first assay, bed bugs were placed onto acetate paper discs with different roughness averages (Ra). Each disc was surrounded by sticky trap adhesive and number of captured bed bugs were recorded. The second assay was set up similarly to the first assay except that the outer portion of the acetate disc had a different Ra than the center. In the third assay, bed bugs were placed into circular acetate arenas where they were surrounded by different Ra treatments. The number of times the bed bugs contacted the Ra treatment but did not cross onto the treatment was recorded. Results of these assays showed that as the acetate surfaces got smoother (lower Ra), bed bugs were more likely to get trapped in sticky trap adhesives but also less likely to travel across the smoother surfaces they encountered. A sticky trap design with a smooth plastic film around the adhesive was tested in the field to see if it could capture bed bugs in apartments with known bed bug activity. This trap was not only able to capture bed bugs but was also able to detect unknown German cockroach, Blattela germanica, infestations. Sticky trap designs with smooth surfaces around an adhesive could be used to monitor not only bed bugs but also German cockroaches.