bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Educational Technologies in CSR Perception of Tourism Education: The Comparative Analysis of E-Learning and M-Learning Tools as Moderators
Authors: John Thomas Basil , Khalil Tarek , Joseph Nisha
Published: December 2020

The aim of this study is to investigate the effect of educational technologies on the Corporate Social Responsibility (CSR) perception of tourism students and their intention to work in the tourism business industry. By improving education programs with an investment in educational technologies, both universities and firms are believed to benefit from growing CSR initiatives, as well as potential young talents for their future business activities. Four-dimensional (economic, legal, ethical and philanthropic dimensions) model of CSR perception is followed. M-learning and E-learning platforms are compared as moderators to ensure the most effective platform for CSR education among the students. The study is conducted with data which is gathered from a total of 397 students who continue their bachelor and associate degrees in different universities in the Gulf nations. It is found that there is a positive relationship between students’ intention to work in the industry and the sub-dimensions of CSR, namely ethical responsibilities, legal responsibilities, and economical responsibilities. Conversely, philanthropic responsibilities had no effect on working intention. In addition, gender difference had no significant impact on working intention of the students in tourism industry. Moreover, it is revealed that e-learning tools are more effective in CSR education.
Drivers of Fire Anomalies in the Brazilian Amazon: Lessons Learned from the 2019 Fire Crisis
Authors: Silveira Marcus V. F. , Petri Caio A. , Broggio Igor S. , Chagas Gabriel O. , Macul Mateus S. , Leite Cândida C. S. S. , Ferrari Edson M. M. , Amim Carolina G. V. , Freitas Ana L. R. , Motta Alline Z. V. , Carvalho Luiza M. E. , Silva Junior Celso H. L. , Anderson Liana O. , Aragão Luiz E. O. C.
Published: December 2020

The 2019 fire crisis in Amazonia dominated global news and triggered fundamental questions about the possible causes behind it. Here we performed an in-depth investigation of the drivers of active fire anomalies in the Brazilian Amazon biome. We assessed a 2003–2019 time-series of active fires, deforestation, and water deficit and evaluated potential drivers of active fire occurrence in 2019, at the biome-scale, state level, and local level. Our results revealed abnormally high monthly fire counts in 2019 for the states of Acre, Amazonas, and Roraima. These states also differed from others by exhibiting in this year extreme levels of deforestation. Areas in 2019 with active fire occurrence significantly greater than the average across the biome had, on average, three times more active fires in the three previous years, six times more deforestation in 2019, and five times more deforestation in the five previous years. Approximately one-third of yearly active fires from 2003 to 2019 occurred up to 1 km from deforested areas in the same year, and one-third of deforested areas in a given year were located up to 500 m from deforested areas in the previous year. These findings provide critical information to support strategic decisions for fire prevention policies and fire combat actions.
The Reading and Writing Connections in Developing Overall L2 Literacy: A Case Study
Authors: Lucas Carmen
Published: December 2020

This study focused on the characteristics of an action-research study concerning an English language reading and writing intervention program in a primary state school, located in northeast Portugal (low-Socio Economic Status setting), where four groups of children (n = 92) participated in the academic year 2019–2020, after the National strategy for foreign languages was launched. The longitudinal study carried out in the action-research methodology, where the teacher, syllabus designer and researcher was the main author, was set out with two main aims. The first was to tackle and to overcome the lack of teacher training for teaching English to young learners in Portuguese primary schools. The second was to prompt intervention, through the design and implementation of a suitable pedagogic approach for teaching English to young learners, in line with Content for Language and Integrated Learning (CLIL) principles, namely English Across the Curriculum, thus fostering the interrelationships between English language reading and writing skills. The findings of the action-research reading intervention program demonstrate that it is possible to overcome such barriers as pupils’ negative attitudes and counteract the damaging effects of poverty in foreign language literacy development by exposing them to children’s picturebooks/storybooks, thus fostering the overall literacy development. The key concepts explored in these books, being associated with primary key curriculum themes are an effective way to establish clear positive connections among English language reading and writing relationships. In addition, the current study also sheds light on how primary English language school teachers can design suitable pedagogic approaches to foster overall literacy development, thus advancing innovative teacher training opportunities.
Cardiotoxicity of Novel Targeted Hematological Therapies
Authors: Giudice Valentina , Vecchione Carmine , Selleri Carmine
Published: December 2020

Chemotherapy-related cardiac dysfunction, also known as cardiotoxicity, is a group of drug-related adverse events negatively affecting myocardial structure and functions in patients who received chemotherapy for cancer treatment. Clinical manifestations can vary from life-threatening arrythmias to chronic conditions, such as heart failure or hypertension, which dramatically reduce quality of life of cancer survivors. Standard chemotherapy exerts its toxic effect mainly by inducing oxidative stress and genomic instability, while new targeted therapies work by interfering with signaling pathways important not only in cancer cells but also in myocytes. For example, Bruton’s tyrosine kinase (BTK) inhibitors interfere with class I phosphoinositide 3-kinase isoforms involved in cardiac hypertrophy, contractility, and regulation of various channel forming proteins; thus, off-target effects of BTK inhibitors are associated with increased frequency of arrhythmias, such as atrial fibrillation, compared to standard chemotherapy. In this review, we summarize current knowledge of cardiotoxic effects of targeted therapies used in hematology.
Effect of Ion Corrosion on 517 Phase Stability
Authors: Wang Guijia , Hu Zhiqi , Chang Jun , Guan Yan , Zhang Tingting , Bi Wanli
Published: December 2020

The main hydration product and source of strength of magnesium oxysulfate cement is 5Mg(OH)2·MgSO4·7H2O (known as the 517 phase). Hardened pastes containing 92.38% of the 517 phase were synthesized in this study, and the influence of different types of chloride solutions on the stability and compressive strength of the 517 phase was investigated. X-ray diffraction and the Rietveld method were used to investigate the 517 phase transition in chloride solutions. Ion chromatography and inductively coupled plasma spectrometry were used to analyze the ion concentrations of the chloride solutions. Scanning electron microscopy and mercury injection porosimetry were used to investigate the effect of ion erosion on the microstructure and pore size distribution. The results showed that the crystal structure of 517 phase remained stable upon immersion in chloride solutions (except for the CaCl2 solution) up to 28 days, and there was no discernible attenuation in the compressive strength of the hardened pastes. Immersion of the 517 phase in CaCl2 solution for 28 days caused Ca2+ ions to combine with SO42− groups to generate CaSO4·2H2O, thereby decomposing the 517 phase. An increase in the concentration of magnesium and sulfate ions in the immersion solutions confirmed the decomposition of the 517 phase. Gel-like Mg(OH)2 was observed in the microstructure of the decomposed 517 phase, and the decomposition of the 517 phase increased the porosity of the hardened pastes.
Improved Predictability of Microstructure Evolution during Hot Deformation of Titanium Alloys
Authors: Buzolin Ricardo Henrique , Miller Branco Ferraz Franz , Lasnik Michael , Krumphals Alfred , Poletti Maria Cecilia
Published: December 2020

Two different mesoscale models based on dislocation reactions are developed and applied to predict both the flow stress and the microstructure evolution during the hot deformation of titanium alloys. Three distinct populations of dislocations, named mobile, immobile, and wall dislocations, describe the microstructure, together with the crystal misorientation and the densities of boundaries. A simple model consisting of production and recovery terms for the evolution of dislocations is compared with a comprehensive model that describes the reactions between different type of dislocations. Constitutive equations connect the microstructure evolution with the flow stresses. Both models consider the formation of a high angle grain boundary by continuous dynamic recrystallization due to progressive lattice rotation. The wall dislocation density evolution is calculated as a result of the subgrain size and boundary misorientation distribution evolutions. The developed models are applied to two near-β titanium alloys, Ti-5553 and Ti-17, and validated for use in hot compression experiments. The differences in the predictability between the developed models are discussed for the flow stress, dislocation densities and microstructure evolutions. Only the comprehensive model can predict the different reactions and their contributions to the evolution of mobile and immobile dislocation densities. The comprehensive model also allows for correlating the elastic strain rate with the softening and hardening kinetics. Despite those differences, the selection of the model used has a small influence on the overall prediction of the subgrain size and the fraction of high angle grain boundaries.
On the Ablation Behavior of Carbon Fiber-Reinforced Plastics during Laser Surface Treatment Using Pulsed Lasers
Authors: Gebauer Jana , Burkhardt Maximilian , Franke Volker , Lasagni Andrés Fabián
Published: December 2020

This contribution discusses the ablation phenomena observed during laser treatment of carbon fiber-reinforced plastics (CFRPs) with pulsed lasers observed employing laser sources with wavelengths of 355 nm, 1064 nm and 10.6 µm and pulse durations from picoseconds (11 ps) to microseconds (14 µs) are analyzed and discussed. In particular, the threshold fluence of the matrix material epoxy (EP) and the damage threshold of CFRP were calculated. Moreover, two general surface pretreatment strategies are investigated, including selective matrix removal and structure generation through indentation (ablation of both, matrix material and fibers) with a cross-like morphology. The surfaces obtained after the laser treatment are characterized by means of optical and scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy is employed for the analysis of composite and constituent materials epoxy and carbon fibers. As a result, different ablation mechanisms, including evaporation and delamination are observed, depending on the employed laser wavelength and pulse duration. For both 355 nm and 1064 nm wavelength, the laser radiation produces only partial ablation of the carbon fibers due to their higher absorption coefficient compared to the epoxy matrix. Although a selective matrix removal without residues is achieved using the pulsed CO2 laser. Differently, both constituent materials are ablated with the nanosecond pulsed UV laser, producing indentations. The sum of the investigations has shown that existing theories of laser technology, such as the ablation threshold according to Liu et al., can be applied to composite materials only to a limited extent. Furthermore, it has been found that the pronounced heterogeneity of CFRP mostly leads to an inhomogeneous ablation result, both when creating grooves and during selective matrix removal, where the carbon fibers influence the ablation result by their thermal conductivity, depending on fiber direction. Finally, despite the material inhomogeneity, a scanning strategy has been developed to compensate the heterogeneous ablation results regarding structure depth, width and heat affected zone.
Possible Applications of Additive Manufacturing Technologies in Shipbuilding: A Review
Authors: Ziółkowski Marcin , Dyl Tomasz
Published: December 2020

3D printing conquers new branches of production due to becoming a more reliable and professional method of manufacturing. The benefits of additive manufacturing such as part optimization, weight reduction, and ease of prototyping were factors accelerating the popularity of 3D printing. Additive manufacturing has found its niches, inter alia, in automotive, aerospace and dentistry. Although further research in those branches is still required, in some specific applications, additive manufacturing (AM) can be beneficial. It has been proven that additively manufactured parts have the potential to out perform the conventionally manufactured parts due to their mechanical properties; however, they must be designed for specific 3D printing technology, taking into account its limitations. The maritime industry has a long-standing tradition and is based on old, reliable techniques; therefore it implements new solutions very carefully. Besides, shipbuilding has to face very high classification requirements that force the use of technologies that guarantee repeatability and high quality. This paper provides information about current R&D works in the field of implementing AM in shipbuilding, possible benefits, opportunities and threats of implementation.
Predicting Benzene Concentration Using Machine Learning and Time Series Algorithms
Authors: Menéndez García Luis Alfonso , Sánchez Lasheras Fernando , García Nieto Paulino José , Álvarez de Prado Laura , Bernardo Sánchez Antonio
Published: December 2020

Benzene is a pollutant which is very harmful to our health, so models are necessary to predict its concentration and relationship with other air pollutants. The data collected by eight stations in Madrid (Spain) over nine years were analyzed using the following regression-based machine learning models: multivariate linear regression (MLR), multivariate adaptive regression splines (MARS), multilayer perceptron neural network (MLP), support vector machines (SVM), autoregressive integrated moving-average (ARIMA) and vector autoregressive moving-average (VARMA) models. Benzene concentration predictions were made from the concentration of four environmental pollutants: nitrogen dioxide (NO2), nitrogen oxides (NOx), particulate matter (PM10) and toluene (C7H8), and the performance measures of the model were studied from the proposed models. In general, regression-based machine learning models are more effective at predicting than time series models.
Evaluating Art Licensing for Digital Archives Using Fuzzy Integral
Authors: Chiu Yu-Jing , Hu Yi-Chung , Du Jia-Jen , Li Chung-Wei , Ken Yen-Wei
Published: December 2020

Determining how to evaluate the art licensing model is a complex multi-criteria decision making (MCDM) problem. This study explores the critical factors influencing art licensing in the art and creative industry because this industry has potential contributions to a high value-added cultural economy in Taiwan. Due to the high cultural value of the National Palace Museum and the uniqueness of its cultural relics, this study takes it as an empirical case. The decision hierarchy is constructed based on a literature review and expert interviews. Furthermore, we used the fuzzy integral to calculate the weights and overall performance of the three licensing models. The empirical results illustrate that both brand licensing and image licensing groups emphasized the aspect of market environment as the primary consideration for licensing model selection. In particular, respondents considered that the adoption of a brand licensing model could facilitate the achievement of better overall performance value. The reason is that brand licensing made the company’s brand visible by imprinting the brand names of both the National Palace Museum and the company on products. This study can be used as reference model for the evaluation of art licensing by enterprises in the future.
Homogenization of a 2D Tidal Dynamics Equation
Authors: Cardone Giuseppe , Fouetio Aurelien , Woukeng Jean Louis
Published: December 2020

This work deals with the homogenization of two dimensions’ tidal equations. We study the asymptotic behavior of the sequence of the solutions using the sigma-convergence method. We establish the convergence of the sequence of solutions towards the solution of an equivalent problem of the same type.
Herring Milt and Herring Milt Protein Hydrolysate Are Equally Effective in Improving Insulin Sensitivity and Pancreatic Beta-Cell Function in Diet-Induced Obese- and Insulin-Resistant Mice
Authors: Wang Yanwen , Nair Sandhya , Gagnon Jacques
Published: December 2020

Although genetic predisposition influences the onset and progression of insulin resistance and diabetes, dietary nutrients are critical. In general, protein is beneficial relative to carbohydrate and fat but dependent on protein source. Our recent study demonstrated that 70% replacement of dietary casein protein with the equivalent quantity of protein derived from herring milt protein hydrolysate (HMPH; herring milt with proteins being enzymatically hydrolyzed) significantly improved insulin resistance and glucose homeostasis in high-fat diet-induced obese mice. As production of protein hydrolysate increases the cost of the product, it is important to determine whether a simply dried and ground herring milt product possesses similar benefits. Therefore, the current study was conducted to investigate the effect of herring milt dry powder (HMDP) on glucose control and the associated metabolic phenotypes and further to compare its efficacy with HMPH. Male C57BL/6J mice on a high-fat diet for 7 weeks were randomized based on body weight and blood glucose into three groups. One group continued on the high-fat diet and was used as the insulin-resistant/diabetic control and the other two groups were given the high-fat diet modified to have 70% of casein protein being replaced with the same amount of protein from HMDP or HMPH. A group of mice on a low-fat diet all the time was used as the normal control. The results demonstrated that mice on the high-fat diet increased weight gain and showed higher blood concentrations of glucose, insulin, and leptin, as well as impaired glucose tolerance and pancreatic β-cell function relative to those on the normal control diet. In comparison with the high-fat diet, the replacement of 70% dietary casein protein with the same amount of HMDP or HMPH protein decreased weight gain and significantly improved the aforementioned biomarkers, insulin sensitivity or resistance, and β-cell function. The HMDP and HMPH showed similar effects on every parameter except blood lipids where HMDP decreased total cholesterol and non-HDL-cholesterol levels while the effect of HMPH was not significant. The results demonstrate that substituting 70% of dietary casein protein with the equivalent amount of HMDP or HMPH protein protects against obesity and diabetes, and HMDP is also beneficial to cholesterol homeostasis.
Antimicrobial and Antioxidant Polyketides from a Deep-Sea-Derived Fungus Aspergillus versicolor SH0105
Authors: Yang Lu-Jia , Peng Xiao-Yue , Zhang Ya-Hui , Liu Zhi-Qing , Li Xin , Gu Yu-Cheng , Shao Chang-Lun , Han Zhuang , Wang Chang-Yun
Published: December 2020

Fifteen polyketides, including four new compounds, isoversiol F (1), decumbenone D (2), palitantin B (7), and 1,3-di-O-methyl-norsolorinic acid (8), along with 11 known compounds (3–6 and 9–15), were isolated from the deep-sea-derived fungus Aspergillus versicolor SH0105. Their structures and absolute configurations were determined by comprehensive spectroscopic data, including 1D and 2D NMR, HRESIMS, and ECD calculations, and it is the first time to determine the absolute configuration of known decumbenone A (6). All of these compounds were evaluated for their antimicrobial activities against four human pathogenic microbes and five fouling bacterial strains. The results indicated that 3,7-dihydroxy-1,9-dimethyldibenzofuran (14) displayed obvious inhibitory activity against Staphylococcus aureus (ATCC 27154) with the MIC value of 13.7 μM. In addition, the antioxidant assays of the isolated compounds revealed that aspermutarubrol/violaceol-I (15) exhibited significant 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity with the IC50 value of 34.1 μM, and displayed strong reduction of Fe3+ with the ferric reducing antioxidant power (FRAP) value of 9.0 mM under the concentration of 3.1 μg/mL, which were more potent than ascorbic acid.
Performance of the IOTA ADNEX Model on Selected Group of Patients with Borderline Ovarian Tumours
Authors: Gaurilcikas Adrius , Gedgaudaite Migle , Cizauskas Arvydas , Atstupenaite Vaida , Paskauskas Saulius , Gaurilcikiene Dovile , Birzietis Tomas , Vaitkiene Daiva , Nadisauskiene Ruta Jolanta
Published: December 2020

Background and objectives: ultrasound is considered to be the primary tool for preoperative assessment of ovarian masses; however, the discrimination of borderline ovarian tumours (BOTs) is challenging, and depends highly on the experience of the sonographer. The Assessment of Different NEoplasias in the adneXa (ADNEX) model is considered to be a valuable diagnostic tool for preoperative assessment of ovarian masses; however, its performance for BOTs has not been widely studied, due to the low prevalence of these tumours. The aim of this study was to evaluate the performance of ADNEX model for preoperative diagnosis of BOTs. Methods: retrospective analysis of preoperative ultrasound datasets of patients diagnosed with BOTs on the final histology after performed surgery was done at a tertiary oncogynaecology centre during the period of 2012–2018. Results: 85 patients were included in the study. The performance of ADNEX model based on absolute risk (AR) improved with the selection of a more inclusive cut-off value, varying from 47 (60.3%) correctly classified cases of BOTs, with the selected cut-off of 20%, up to 67 (85.9%) correctly classified cases of BOTs with the cut-off value of 3%. When relative risk (RR) was used to classify the tumours, 59 (75.6%) cases were identified correctly. Forty (70.2%) cases of serous and 16 (72.7%) cases of mucinous BOTs were identified when AR with a 10% cut-off value was applied, compared to 44 (77.2%) and 15 (68.2%) cases of serous and mucinous BOTs, correctly classified by RR. The addition of Ca125 improved the performance of ADNEX model for all BOTs in general, and for different subtypes of BOTs. However, the differences were insignificant. Conclusions: The International Ovarian Tumour Analysis (IOTA) ADNEX model performs well in discriminating BOTs from other ovarian tumours irrespective of the subtype. The calculation based on RR or AR with the cut-off value of at least 10% should be used when evaluating for BOTs.
Anaphylaxis: Five Years’ Experience in the Emergency Rooms of Five University Hospitals in Korea
Authors: Chung Bo Young , Um Ji Young , Kim Jin Cheol , Kang Seok Young , Jung Min Je , Kim Hye One , Park Chun Wook
Published: December 2020

Background: Anaphylaxis is an allergic disease that requires special handling due to its potential fatality. Recent epidemiological data indicate that the incidence of anaphylaxis is rising. However, actual data on the prevalence or causes of anaphylaxis in Korea are limited. Methods: The emergency room attendees diagnosed with anaphylaxis between 2011 and 2015 in five university hospitals were included. Medical records were reviewed retrospectively. Results: During the 5 years, a total of 505 subjects were diagnosed with anaphylaxis. Respiratory presentations were more common in children than in adults, while adults presented more frequently with cardiovascular symptoms. Intraoral angioedema was more often observed in the countryside than in the city. Insect stings/bites were more common in the countryside than in the city. Drugs were much more common in adults than in children. In the countryside, the frequency of anaphylaxis was higher in summer and autumn than in spring and winter. The use of corticosteroids was less common in children than in adults, while children more frequently got treatment with inhaled beta 2 agonist. Conclusions: The principal causes of anaphylaxis in Korean patients were food, drugs, and stings/bites. The cause, clinical features and management of anaphylaxis were significantly different depending on age and region. These real-world data on anaphylaxis could be helpful to deepen that understanding of this condition for physicians and patients.
Skills Requirements for the European Machine Tool Sector Emerging from Its Digitalization
Authors: Akyazi Tugce , Goti Aitor , Oyarbide-Zubillaga Aitor , Alberdi Elisabete , Carballedo Roberto , Ibeas Rafael , Garcia-Bringas Pablo
Published: December 2020

The machine tool industry, which is the starting point of all the metal producing activities, is presently undergoing rapid and continuous changes as a result of the fourth industrial revolution Industry 4.0. Manufacturing models are profoundly transforming with emerging digitalization. Smart technologies like artificial intelligence (AI), big data, the Internet of Things (IoT), digital twin, allow the machine tool companies to optimize processes, increase efficiency and reduce waste through a new phase of automation. These technologies, as well, enable the machine tool producers to reach the aim of creating products with improved performance, extended life, high reliability that are eco-efficient. Therefore, Industry 4.0 could be perceived as an invaluable opportunity for the machine tool sector, only if the sector has a competent workforce capable of handling the implementation of new business models and technological developments. The main condition to create this highly qualified workforce is reskilling and upskilling of the current workforce. Once we define the expected evolution of skills requirements, we can clarify the skills mismatch between the workers and job profiles. Only then, we can reduce them by delivering well-developed trainings. For this purpose, this article identifies the current and foreseen skills requirements demanded by the machine tool industry workforce. To this end, we generated an integrated database for the sector with the present and prospective skills needs of the metal processing sector professionals. The presented sectoral database is a fundamental structure that will make the sector acquire targeted industrial reforms. It can also be an essential instrument for machine tool companies, policymakers, academics and education or training centers to build well-designed and effective training programs to enhance the skills of the labor force.
Comparative Analysis of Milk Triglycerides Profile between Jaffarabadi Buffalo and Holstein Friesian Cow
Authors: Verma Aparna , Meitei Ningombam Sanjib , Gajbhiye Prakash U. , Raftery Mark J. , Ambatipudi Kiran
Published: December 2020

Milk lipids are known for a variety of biological functions, however; little is known about compositional variation across breeds, especially for Jaffarabadi buffalo, an indigenous Indian breed. Systematic profiling of extracted milk lipids was performed by mass spectrometry across summer and winter in Holstein Friesian cow and Jaffarabadi buffalo. Extensive MS/MS spectral analysis for the identification (ID) of probable lipid species using software followed by manual verification and grading of each assigned lipid species enabled ID based on (a) parent ion, (b) head group, and (c) partial/full acyl characteristic ions for comparative profiling of triacylglycerols between the breeds. Additionally, new triacylglycerol species with short-chain fatty acids were reported by manual interpretation of MS/MS spectra and comparison with curated repositories. Collectively, 1093 triacylglycerol species belonging to 141 unique sum compositions between the replicates of both the animal groups were identified. Relative quantitation at sum composition level followed by statistical analyses revealed changes in relative abundances of triacylglycerol species due to breed, season, and interaction effect of the two. Significant changes in triacylglycerols were observed between breeds (81%) and seasons (59%). When the interaction effect is statistically significant, a higher number of triacylglycerols species in Jaffarabadi has lesser seasonal variation than Holstein Friesian.
A New Approach for the Production of Selenium-Enriched and Probiotic Yeast Biomass from Agro-Industrial by-Products in a Stirred-Tank Bioreactor
Authors: Martiniano Sabrina Evelin , Fernandes Letícia Alves , Alba Edith Mier , Philippini Rafael Rodrigues , Tabuchi Stephanie Caroline Tavares , Kieliszek Marek , Santos Júlio César dos , da Silva Silvio Silvério
Published: December 2020

The production of biomolecules using agro-industrial by-products as feedstock is a growing trend worldwide. Selenium (Se) is a trace element essential for health, and the Se-enrichment of yeast biomass can enhance its benefits. This study investigated the feasibility of the production of Saccharomyces cerevisiae Se-enriched biomass using a medium composed of corn bran and soybean bran acid hydrolysates as carbon and nitrogen sources in a stirred-tank reactor. After hydrolysis, hydrolysates presented complex composition and high concentrations of sugars, proteins, and minerals. The use of a stirred-tank bioreactor leads to the production of 9 g/L S. cerevisiae biomass enriched with 236.93 μg/g Se, and 99% cell viability. Likewise, the combination of sugarcane molasses and soybean bran hydrolysate was effective for cell growth of a probiotic strain of S. cerevisiae with a 24.08% β-glucan content. The results demonstrated that starchy acid hydrolysates are low-cost and efficient substrates for the production of yeast biomass and derivate products and may contribute to further studies for a sustainable development of biorefinery technologies.
A Comparative Study of Laser-Induced Graphene by CO2 Infrared Laser and 355 nm Ultraviolet (UV) Laser
Authors: Wang Liyong , Wang Zhiwen , Bakhtiyari Ali Naderi , Zheng Hongyu
Published: December 2020

Laser-induced graphene (LIG) is an emerging technique for producing few-layer graphene or graphene-like material that has recently received increasing attention, due to its unique advantages. Subsequently, a variety of lasers and materials have been used to fabricate LIG using this technique. However, there is a lack of understanding of how different lasers (wavelengths) perform differently in the LIG conversion process. In this study, the produced LIG on polyimide (PI) under a locally water-cooled condition using a 10.6 μm CO2 infrared laser and a 355 nm ultraviolet (UV) laser are compared. The experimental investigations reveal that under the same UV and CO2 laser fluence, the ablation of PI show different results. Surface morphologies with micron-sized and nanometer pores were formed by the UV laser under different laser fluences, whereas micron-sized pores and sheet structure with fewer pores were produced by the CO2 laser. Energy dispersive spectrometry and three-dimensional topography characterization indicate that the photochemical effects were also involved in the LIG conversion with UV laser irradiation. It is also observed through experiments that the photothermal effect contributed to the formation of LIG under both lasers, and the LIG formed on PI substrates by the CO2 laser showed better quality and fewer layers.
Effect of the Pressure of Reaction Gases on the Growth of Single-Crystal Graphene on the Inner Surfaces of Copper Pockets
Authors: Yang Kaiqiang , Liu Jianlong , Jiang Ruirui , Gong Yubin , Zeng Baoqing , Yi Zichuan , Gao Qingguo , Yang Jianjun , Chi Feng , Liu Liming
Published: December 2020

Single-crystal graphene has attracted much attention due to its excellent electrical properties in recent years, and many growth methods have been proposed, including the copper pockets method. In the copper pockets method, a piece of copper foil is folded into a pocket and put into a chemical vapor deposition (CVD) system for the growth of graphene. The dynamic balance of evaporation and deposition of copper on the inner surfaces of the copper pockets avoids high surface roughness caused by the evaporation of copper in open space, such as the outer surfaces of copper pockets. Much lower partial pressure of methane in the copper pockets and lower surface roughness reduce the nucleation density of graphene and increase the size of single-crystal graphene. It is found that the growth pressure is closely related to the size of single-crystal graphene prepared by the copper pockets method; the higher the growth pressure, the larger the size of single-crystal graphene. It is also found that the growth pressure has an effect on the inner surface roughness of the copper pockets, but the effect is not significant. The main factor affecting the size of the single-crystal graphene is the change in the volume of the copper pockets caused by the change in the growth pressure, and the volume of the copper pockets determines the content of methane in the copper pockets. According to the above law, the size of single-crystal graphene prepared by the copper pockets method can be enlarged by increasing the growth pressure. The size of single-crystal graphene can be enlarged in a wide range as the growth pressure can be increased in a wide range. In our experiments, when the growth pressure reached 450 Pa, single-crystal graphene with a diameter of 450 μm was prepared.
Combination of Colistin and Azidothymidine Demonstrates Synergistic Activity against Colistin-Resistant, Carbapenem-Resistant Klebsiella pneumoniae
Authors: Chang Ya-Ting , Yang Tsung-Ying , Lu Po-Liang , Lin Shang-Yi , Wang Liang-Chun , Wang Sheng-Fan , Hsieh Ya-Ju , Tseng Sung-Pin
Published: December 2020

Carbapenem-resistant Enterobacteriaceae (CRE) is listed as an urgent threat by the World Health Organization because of the limited therapeutic options, rapid evolution of resistance mechanisms, and worldwide dissemination. Colistin is a common backbone agent among the “last-resort” antibiotics for CRE; however, its emerging resistance among CRE has taken the present dilemma to the next level. Azidothymidine (AZT), a thymidine analog used to treat human immunodeficiency virus/acquired immunodeficiency syndrome, has been known to possess antibacterial effects against Enterobacteriaceae. In this study, we investigated the combined effects of AZT and colistin in 40 clinical isolates of colistin-resistant, carbapenem-resistant K. pneumoniae (CCRKP). Eleven of the 40 isolates harbored Klebsiella pneumoniae carbapenemase. The in vitro checkerboard method and in vivo nematode killing assay both revealed synergistic activity between the two agents, with fractional inhibitory concentration indexes of ≤0.5 in every strain. Additionally, a significantly lower hazard ratio was observed for the nematodes treated with combination therapy (0.288; p < 0.0001) compared with either AZT or colistin treatment. Toxicity testing indicated potentially low toxicity of the combination therapy. Thus, the AZT–colistin combination could be a potentially favorable therapeutic option for treating CCRKP.
Retroviral Restriction Factors and Their Viral Targets: Restriction Strategies and Evolutionary Adaptations
Authors: Boso Guney , Kozak Christine A.
Published: December 2020

The evolutionary conflict between retroviruses and their vertebrate hosts over millions of years has led to the emergence of cellular innate immune proteins termed restriction factors as well as their viral antagonists. Evidence accumulated in the last two decades has substantially increased our understanding of the elaborate mechanisms utilized by these restriction factors to inhibit retroviral replication, mechanisms that either directly block viral proteins or interfere with the cellular pathways hijacked by the viruses. Analyses of these complex interactions describe patterns of accelerated evolution for these restriction factors as well as the acquisition and evolution of their virus-encoded antagonists. Evidence is also mounting that many restriction factors identified for their inhibition of specific retroviruses have broader antiviral activity against additional retroviruses as well as against other viruses, and that exposure to these multiple virus challenges has shaped their adaptive evolution. In this review, we provide an overview of the restriction factors that interfere with different steps of the retroviral life cycle, describing their mechanisms of action, adaptive evolution, viral targets and the viral antagonists that evolved to counter these factors.
Neutrophil Gelatinase-Associated Lipocalin (NGAL) Is Related with the Proteinuria Degree and the Microscopic Kidney Findings in Leishmania-Infected Dogs
Authors: Peris María Paz , Morales Mariano , Ares-Gómez Sonia , Esteban-Gil Adriana , Gómez-Ochoa Pablo , Gascón Manuel , Moreno Bernardino , Castillo Juan Antonio
Published: December 2020

Early diagnosis of renal damage in Leishmania infected dogs may allow appropriate treatments and prevent some deaths. This study investigates neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker of kidney disease in dogs experimentally infected with Leishmania infantum. Serum, urine, and kidney samples were collected from 30 infected beagle dogs and six uninfected control dogs. Based on proteinuria and azotemia values, dogs were initially classified. NGAL was measured in urine and serum samples. Then, the urinary NGAL to creatinine ratio (uNGAL/C) was calculated. Kidney samples were taken for histopathological studies, and the dogs were classified according to the severity of glomerular and tubulointerstitial lesions. In Leishmania-infected dogs, the uNGAL/C was significantly higher in proteinuric non-azotemic dogs compared with non-proteinuric non-azotemic dogs (p = 0.038). Serum NGAL (sNGAL) concentration did not differ between groups. Microscopic studies revealed several degrees of glomerulonephritis and slight focal lymphoplasmacytic interstitial nephritis in 89% and 55% of infected dogs, respectively. Urinary protein to creatinine ratio (UPC) and uNGAL/C were significantly higher in dogs with affected glomeruli compared to infected dogs without renal lesions (p = 0.045 and p = 0.043, respectively). The results show that uNGAL/C correlates with proteinuria and the presence of moderate glomerular lesions in non-azotemic dogs experimentally infected with L. infantum.
Soehngenia longivitae sp. nov., a Fermenting Bacterium Isolated from a Petroleum Reservoir in Azerbaijan, and Emended Description of the Genus Soehngenia
Authors: Nazina Tamara N. , Bidzhieva Salimat K. , Grouzdev Denis S. , Sokolova Diyana S. , Tourova Tatyana P. , Parshina Sofiya N. , Avtukh Alexander N. , Poltaraus Andrey B. , Talybly Azhdar K.
Published: December 2020

A methanogenic enrichment growing on a medium with methanol was obtained from a petroleum reservoir (Republic of Azerbaijan) and stored for 33 years without transfers to fresh medium. High-throughput sequencing of the V4 region of the 16S rRNA gene revealed members of the genera Desulfovibrio, Soehngenia, Thermovirga, Petrimonas, Methanosarcina, and Methanomethylovorans. A novel gram-positive, rod-shaped, anaerobic fermentative bacterium, strain 1933PT, was isolated from this enrichment and characterized. The strain grew at 13–55 °C (optimum 35 °C), with 0–3.0% (w/v) NaCl (optimum 0–2.0%) and in the pH range of 6.7–8.0 (optimum pH 7.0). The 16S rRNA gene sequence similarity, the average nucleotide identity (ANI) and in silico DNA–DNA hybridization (dDDH) values between strain 1933PT and the type strain of the most closely related species Soehngenia saccharolytica DSM 12858T were 98.5%, 70.5%, and 22.6%, respectively, and were below the threshold accepted for species demarcation. Genome-based phylogenomic analysis and physiological and biochemical characterization of the strain 1933PT (VKM B-3382T = KCTC 15984T) confirmed its affiliation to a novel species of the genus Soehngenia, for which the name Soehngenia longivitae sp. nov. is proposed. Genome analysis suggests that the new strain has potential in the degradation of proteinaceous components.
Effects of Different Drug Combinations in Immunodeficient Mice Infected with an Influenza A/H3N2 Virus
Authors: Mhamdi Zeineb , Fausther-Bovendo Hugues , Uyar Olus , Carbonneau Julie , Venable Marie-Christine , Abed Yacine , Kobinger Gary , Boivin Guy , Baz Mariana
Published: December 2020

The prolonged treatment of immunosuppressed (IS) individuals with anti-influenza monotherapies may lead to the emergence of drug-resistant variants. Herein, we evaluated oseltamivir and polymerase inhibitors combinations against influenza A/H3N2 infections in an IS mouse model. Mice were IS with cyclophosphamide and infected with 3 × 103 PFU of a mouse-adapted A/Switzerland/9715293/2013 (H3N2) virus. Forty-eight hours post-infection, the animals started oseltamivir, favipiravir or baloxavir marboxil (BXM) as single or combined therapies for 10 days. Weight losses, survival rates and lung viral titers (LVTs) were determined. The neuraminidase (NA) and polymerase genes from lung viral samples were sequenced. All untreated animals died. Oseltamivir and favipiravir monotherapies only delayed mortality (the mean day to death (MDD) of 21.4 and 24 compared to 11.4 days for those untreated) while a synergistic improvement in survival (80%) and LVT reduction was observed in the oseltamivir/favipiravir group compared to the oseltamivir group. BXM alone or in double/triple combination provided a complete protection and significantly reduced LVTs. Oseltamivir and BXM monotherapies induced the E119V (NA) and I38T (PA) substitutions, respectively, while no resistance mutation was detected with combinations. We found that the multiple dose regimen of BXM alone provided superior benefits compared to oseltamivir and favipiravir monotherapies. Moreover, we suggest the potential for drug combinations to reduce the incidence of resistance.
Tick Fauna and Associated Rickettsia, Theileria, and Babesia spp. in Domestic Animals in Sudan (North Kordofan and Kassala States)
Authors: Springer Andrea , Shuaib Yassir Adam , Isaa Makarim Habib , Ezz-Eldin Malaz Isam-Eldin , Osman Abdinasir Yusuf , Yagoub Idris Ahmed , Abdalla Mohamed Abdalsalam , Bakiet Amel Omer , Mohmed-Noor Saad El-Tiab , Schaper Sabine , Rieß Ramona , Dobler Gerhard , Strube Christina , Bakkes Deon K. , Chitimia-Dobler Lidia
Published: December 2020

Ticks and tick-borne diseases (TBDs) have a major economic impact on animal production worldwide. In the present study, 2410 ticks were collected from January to August 2017 from livestock and other domestic animals in North Kordofan and Kassala, Sudan, for species identification and investigation of Rickettsia spp. and piroplasms, either individually or as pools containing up to 10 ticks by molecular methods. In total, 13 tick species were identified by morphology and 16S rDNA sequencing. The most frequent tick species were Hyalomma impeltatum (24.90%), Rhipicephalus evertsi evertsi (18.84%), Amblyomma lepidum (16.06%), and Rhipicephalus camicasi (12.49%). A pan-Rickettsia real-time PCR revealed an overall minimum infection rate (MIR) with Rickettsia spp. of 5.64% (136 positive tick pools/2410 total ticks). Rickettsia africae and Rickettsia aeschlimannii were the most frequently identified species by sequencing. Furthermore, the following highly pathogenic livestock parasites were detected: Theileria annulata, Theileria lestoquardi, Theileria equi, and Babesia caballi. The present study documented Rhipicephalus afranicus as well as Rickettsia conorii israelensis, Rickettsia massiliae, and Babesia pecorum for the first time in Sudan. These findings are significant for the animal production sector as well as in terms of One Health, as the detected Rickettsia spp. can cause serious illness in humans.
Effect of α-Hemolysin Producing E. coli in Two Different Mouse Strains in a DSS Model of Inflammatory Bowel Disease
Authors: Mirsepasi-Lauridsen Hengameh Chloé , Struve Carsten , Petersen Andreas Munk , Krogfelt Karen Angeliki
Published: December 2020

Background: Phylogroup B2 Escherichia coli have been associated with ulcerative colitis (UC). In this study, we aimed to compare colonization with the UC-associated E. coli p19A in different mice strains, to investigate the role of alpha hemolysin in a UC mouse model. Methods: In this study, Sigirr −/− and C57BL/6 mice were chosen, and UC was induced by adding dextran sulfate sodium (DSS) to the drinking water. The mice were pre-treated with ciprofloxacin. p19A expressing luminescence and GFP, alpha-hemolysin knock out p19A-ΔhlyI II, and non-pathogenic lab E. coli DH10B were cultured in LB broth, and orally gavaged into the mice. Colonization with p19A WT was visualized using an in vivo imaging system. Results: p19A WT colonized the colon, ileum, Peyer’s patches, liver, and spleen of infected C57BL/6 and Sigirr −/− mice. A total of 99% of the p19A WT infected C57BL/6 mice and 29% of the p19A WT infected Sigirr −/− mice survived to the 4th post infection day. Conclusion: UC-associated E. coli p19A WT colonized the intestines of DSS-treated mice and caused extra-intestinal infection. Hemolysin is an important factor in this pathogenesis, since isogenic hemolysin mutants did not cause the same inflammation.
Unconventional Secretion of Nigerolysins A from Aspergillus Species
Authors: Kraševec Nada , Novak Maruša , Barat Simona , Skočaj Matej , Sepčić Kristina , Anderluh Gregor
Published: December 2020

Aegerolysins are small lipid-binding proteins particularly abundant in fungi. Aegerolysins from oyster mushrooms interact with an insect-specific membrane lipid and, together with MACPF proteins produced by the same organism, form pesticidal pore-forming complexes. The specific interaction with the same membrane lipid was recently demonstrated for nigerolysin A2 (NigA2), an aegerolysin from Aspergillus niger. In Aspergillus species, the aegerolysins were frequently found as secreted proteins, indicating their function in fungal defense. Using immunocytochemistry and live-cell imaging we investigated the subcellular localization of the nigerolysins A in A. niger, while their secretion was addressed by secretion prediction and Western blotting. We show that both nigerolysins A are leaderless proteins that reach the cell exterior by an unconventional protein secretion. NigA proteins are evenly distributed in the cytoplasm of fungal hyphae. A detailed bioinformatics analysis of Aspergillus aegerolysins suggests that the same function occurs only in a limited number of aegerolysins. From alignment, analysis of chromosomal loci, orthology, synteny, and phylogeny it follows that the same or a similar function described for pairs of pesticidal proteins of Pleurotus sp. can be expected in species of the subgenus Circumdati, section Nigri, series Nigri, and some other species with adjacent pairs of putative pesticidal proteins.