bluuebunny/crossref_metadata_2025 huggingface dataset:
Revisiting the Pathogenesis of Type 1 Diabetes: Importance of Neural Input to Pancreatic Islets and the Therapeutic Capability of Stem Cell Educator TM Therapy to Restore Their Integrity
Authors: Zhao Yong , Veysman Boris
Published: February 2023

Type 1 diabetes (T1D) is an autoimmune disease with a shortage of islet β cells. To date, the etiology of T1D remains elusive. Increasing clinical evidence and animal studies demonstrate that autoimmune cells are directed against the nervous system of pancreatic islets, contributing to the development of T1D. Therefore, it highlights the necessity to explore novel clinical approaches to fundamentally correct the T1D autoimmunity not only focusing on islet β cells but also on protecting the islet nervous system. This allows the restoration of the integrity of islet innervation and the normal islet β-cell function. To address these issues, we developed a novel technology designated the Stem Cell Educator TM therapy, based on immune education by human cord-blood-derived multipotent stem cells (CB-SC). International amulticenter clinical trials demonstrated its clinical safety and efficacy to treat T1D and other autoimmune diseases. Stem Cell Educator TM therapy may have the potential to revolutionize the treatment of T1D, without the safety and ethical concerns associated with conventional immune and/or stem cell-based therapies.
Comparison among Neuroblastoma Stages Suggests the Involvement of Mitochondria in Tumor Progression
Authors: Cagnin Stefano , Knedlik Tomas , Vianello Caterina , Magalhães Rebelo Ana Paula , De Mario Agnese , Giacomello Marta
Published: February 2023

Neuroblastoma (NB) is the most common extracranial tumor of early childhood and accounts for 15% of all pediatric cancer mortalities. However, the precise pathways and genes underlying its progression are unknown. Therefore, we performed a differential gene expression analysis of neuroblastoma stage 1 and stage 4 + 4S to discover biological processes associated with NB progression. From this preliminary analysis, we found that NB samples (stage 4 + 4S) are characterized by altered expression of some proteins involved in mitochondria function and mitochondria–ER contact sites (MERCS). Although further analyses remain necessary, this review may provide new hints to better understand NB molecular etiopathogenesis, by suggesting that MERCS alterations could be involved in the progression of NB.
Advanced Bionic Attachment Equipment Inspired by the Attachment Performance of Aquatic Organisms: A Review
Authors: Zhang Dexue , Xu Jin , Liu Xuefeng , Zhang Qifeng , Cong Qian , Chen Tingkun , Liu Chaozong
Published: February 2023

In nature, aquatic organisms have evolved various attachment systems, and their attachment ability has become a specific and mysterious survival skill for them. Therefore, it is significant to study and use their unique attachment surfaces and outstanding attachment characteristics for reference and develop new attachment equipment with excellent performance. Based on this, in this review, the unique non-smooth surface morphologies of their suction cups are classified and the key roles of these special surface morphologies in the attachment process are introduced in detail. The recent research on the attachment capacity of aquatic suction cups and other related attachment studies are described. Emphatically, the research progress of advanced bionic attachment equipment and technology in recent years, including attachment robots, flexible grasping manipulators, suction cup accessories, micro-suction cup patches, etc., is summarized. Finally, the existing problems and challenges in the field of biomimetic attachment are analyzed, and the focus and direction of biomimetic attachment research in the future are pointed out.
Characterization of Bipolar Transport in Hf(Te1−xSex)2 Thermoelectric Alloys
Authors: Hwang Seong-Mee , Kim Sang-il , Kim Jeong-Yeon , Heo Minsu , Kim Hyun-Sik
Published: February 2023

Control of bipolar conduction is essential to improve the high-temperature thermoelectric performance of materials for power generation applications. Recently, Hf(Te1−xSex)2 alloys have gained much attention due to their potential use in thermoelectric power generation. Increasing the Se alloying content significantly increases the band gap while decreasing its carrier concentration. These two factors affect bipolar conduction substantially. In addition, the weighted mobility ratio is estimated from the experimental electronic transport properties of Hf(Te1−xSex)2 alloys (x = 0.0, 0.025, 0.25, 0.5, 1.0) by using the Two-Band model. From the bipolar thermal conductivity also calculated using the Two-Band model, we find that it peaks near x = 0.5. The initial bipolar conductivity increase of x < 0.5 is mostly due to the decrease in the weighted mobility ratio and carrier concentration with increasing x. For x > 0.5, the drop in the bipolar conductivity can be understood with significant band gap enlargement.
Effects of a Psychosocial Intervention on the Subjective Experiences of Children Living with Atopic Dermatitis: A Qualitative Study in Hong Kong
Authors: Xie Qian-Wen
Published: February 2023

Nonpharmaceutical interventions are important for addressing the psychosocial needs of children living with atopic dermatitis (AD). The current study aimed to investigate the effects of an integrative body–mind–spirit (IBMS) intervention on the subjective experiences of affected children and explore the mechanisms underlying the effects. Using a drawing-based, qualitative approach, the current study conducted two rounds of interviews with 13 children (aged 8–12 years) diagnosed with moderate or severe AD before and after they attended the IBMS intervention. Data were analyzed by using the thematic analysis method. The IBMS intervention worked by changing participants’ perceptions at the cognitive level, improving their coping strategies at the behavioral level, and constructing their social support network at the environmental level. Cognitive, behavioral, and environmental factors might mediate the relationships between the IBMS intervention and participants’ psychological and physical outcomes. This study highlighted the wider inclusion of child-centered qualitative research in the evaluation of the effects of psychosocial interventions designed for children.
Membranes and Integrated Membrane Operations as Clean Technologies in the Leather Industry
Authors: Drioli Enrico , Cassano Alfredo
Published: February 2023

The leather industry is characterized by the production of a huge amount of wastewater with a high organic/inorganic charge, causing widespread water and soil pollution. Pressure-driven membrane operations and membrane bioreactors have long been proven to be a valid approach for the treatment of tanning wastewaters aimed at the recovery of raw materials as well as for the removal of toxic and environmentally harmful substances. Such processes, opportunely integrated among themselves and/or with conventional physical-chemical and biological treatments, also provide useful protocols for the treatment of global wastewaters with significant advantages in terms of environmental protection, decrease of disposal costs, simplification of cleaning-up processes and saving of water and chemicals. This paper, as the state of the art, attempts to revise the potential and perspectives of membrane-based technologies in the leather industry with related applications in beamhouse, tanning and post-tanning operations as well as in the treatment of global wastewaters.
The Anatomy of a Hybrid In-Person and Virtual Sexual Health Clinic in Oncology
Authors: Matthew Andrew , Guirguis Steven , Incze Taylor , Stragapede Elisa , Peltz Sarah , Yang Gideon , Jamnicky Leah , Elterman Dean
Published: February 2023

Sexual health is compromised by the diagnosis and treatment of virtually all cancer types. Despite the prevalence and negative impact of sexual dysfunction, sexual health clinics are the exception in cancer centers. Consequently, there is a need for effective, efficient, and inclusive sexual health programming in oncology. This paper describes the development of the innovative Sexual Health Clinic (SHC) utilizing a hybrid model of integrated in-person and virtual care. The SHC evolved from a fusion of the in-person and virtual prostate cancer clinics at Princess Margaret. This hybrid care model was adapted to include six additional cancer sites (cervical, ovarian, testicular, bladder, kidney, and head and neck). The SHC is theoretically founded in a biopsychosocial framework and emphasizes interdisciplinary intervention teams, participation by the partner, and a medical, psychological, and interpersonal approach. Virtual visits are tailored to patients based on biological sex, cancer type, and treatment type. Highly trained sexual health counselors facilitate the virtual clinic and provide an additional layer of personalization and a “human touch”. The in-person visits complement virtual care by providing comprehensive sexual health assessment and sexual medicine prescription. The SHC is an innovative care model which has the potential to close the gap in sexual healthcare. The SHC is designed as a transferable, stand-alone clinic which can be shared with cancer centers.
A Novel Electrolytic-Free Quasi-Z-Source Ćuk LED Driver for Automotive Application
Authors: Wang Lei , Hu Wei
Published: February 2023

This paper proposes a novel electrolytic-free quasi-Z-Source Ćuk LED driver for automotive applications. Compared to the traditional Ćuk converter, the first merit of the novel converter is higher gain, which makes it apt to switch between multiple applications. Secondly, the proposed converter combines the inherent characteristics of LED load to operate in a wide range in Continuous Conduction Mode (CCM), so the inductors can assist in energy storage, and only small capacitance is required. Thirdly, inductors can be integrated and use only one core, and capacitors are electrolytic-free, which will benefit integration and long life. All merits are important to automotive application. Detailed analysis and design steps are presented. Then, with the help of the simulation software Saber, several key parts of the converter are simulated. Finally, a prototype controlled by the micro control unit stm32f103c8t6 is built, and the feasibility is verified by the experiment results.
Covert Communication for Integrated Satellite–Terrestrial Relay Networks with Cooperative Jamming
Authors: Wu Zeke , Guo Kefeng , Zhu Shibing
Published: February 2023

We explore the influence of cooperative jamming on covert communication for integrated satellite–terrestrial relay networks (ISTRN) in this article, where one full-duplex relay is utilized to support communications and acts as a jammer to confuse eavesdroppers. A cooperative scheme is first proposed to efficiently achieve covert communication in this specific scenario. Further, the probability of detection error (PDE) is derived. Subsequently, the minimum PDE is calculated using the optimal detection threshold. Moreover, a closed-form expression of outage probability (OP) is acquired to quantify the system’s covert performance. Based on this, we design a power optimization algorithm to enhance the effective covert rate under covertness constraints. Lastly, numerical simulation results are given to verify the efficiency of our cooperative jamming scheme when interrupting the eavesdroppers. Notably, an optimal power allocation factor can be obtained, leading to a higher effective covert rate.
Effect of Trapped Charge Induced by Total Ionizing Dose Radiation on the Top-Gate Carbon Nanotube Field Effect Transistors
Authors: Ding Hongyu , Cui Jiangwei , Zheng Qiwen , Xu Haitao , Gao Ningfei , Xun Mingzhu , Yu Gang , He Chengfa , Li Yudong , Guo Qi
Published: February 2023

The excellent performance and radiation-hardness potential of carbon nanotube (CNT) field effect transistors (CNTFETs) have attracted wide attention. However, top-gate structure CNTFETs, which are often used to make high-performance devices, have not been studied enough. In this paper, the total ionizing dose (TID) effect of the top-gate structure CNTFETs and the influence of the substrate on top-gate during irradiation are studied. The parameter degradation caused by the irradiation- and radiation-damage mechanisms of the top-gate P-type CNTFET were obtained by performing a Co-60 γ-ray irradiation test. The results indicate that the transfer curves of the top-gate P-type CNTFETs shift negatively, the threshold voltage and the transconductance decrease when TID increases, and the subthreshold swing decreases first and then increases with the increase in TID. The back-gate transistor is constructed by using the substrate as a back-gate, and the influence of back-gate bias on the characteristics of the top-gate transistor is tested. We also test the influence of TID irradiation on the characteristics of back-gate transistors, and reveal the effect of trapped charge introduced by radiation on the characteristics of top-gate transistors. In addition, the CNTFETs that we used have obvious hysteresis characteristics. After irradiation, the radiation-induced trapped charges generated in oxide and the OH groups generated by ionization of the CNT adsorbates aggravate the hysteresis characteristics of CNTFET, and the hysteresis window increases with the increase in TID.
CFD Modelling and Analysis for Green Environment of Traditional Buildings
Authors: Li Yangluxi , Chen Lei , Yang Li
Published: February 2023

With the enhancement of people’s awareness of heritage protection, research communities focusing on the natural ventilation of the layouts of ancient buildings have paid more attention to the planning and protection of these buildings. Based on the relationship between the natural ventilation environment and the layout of the building, we can reduce the adverse effects of energy consumption and outdoor wind, improve the environment and quality around the building, and achieve harmony between humans and nature. In this study, Fluent software was used to simulate the wind environment of Xingguo Temple. The advantages of combining computer simulation software with ancient building protection planning are illustrated by comparing the wind environment before and after the temple reconstruction with Fluent software. Through the simulation of the building’s wind environment, some suggestions are put forward for the early layout of the outdoor environment in the ancient building reconstruction planning area.
A Comprehensive Study on the Sustainable Transportation System in India and Lessons to Be Learned from Other Developing Nations
Authors: Mohapatra Subhashree , Mohanachandran Dileep , Dwivedi Gaurav , Kesharvani Sujeet , Harish V. S. K. V. , Verma Shrey , Verma Puneet
Published: February 2023

The wheel was the first form of transportation developed by early people. Increasing population, longer distances, and shorter travel times need more cheap, faster modes of transportation. Environmental issues were never taken into account when choosing the most cost-effective mode of public transportation. Consequently, in the face of global pollution and warming restrictions, all stakeholders choose modes of transportation with little environmental impact. This has led to the development of sustainable transportation infrastructure, particularly in a country as rapidly developing as India. This paper examines the present situation, legislation, and grading systems for sustainable transportation in India and other developing nations. Furthermore, comparisons of India with developing nations of various continents in different aspects are also present. The difference in cost between a private car and a public vehicle indicates which form of transportation should be chosen. The challenges linked with urban and rural Indian roadways are explored, as well as their respective solutions. In order to accomplish sustainable transportation, traffic density and driver conduct are also considered. This study emphasises that sustainability is not just achieved through the use of cleaner fuel or the modification of road materials, although these are vital. Road design, regulatory changes, psychological behaviour, and transit safety are also crucial.
Point-Interval Forecasting for Electricity Load Based on Regular Fluctuation Component Extraction
Authors: Shao Bilin , Yao Zixuan , Qiang Yifan
Published: February 2023

The fluctuation and uncertainty of the electricity load bring challenges to load forecasting. Traditional point forecasting struggles to avoid errors, and pure interval forecasting may cause the problem of too wide an interval. In this paper, we combine point forecasting and interval forecasting and propose a point-interval forecasting model for electricity load based on regular fluctuation component extraction. Firstly, the variational modal decomposition is combined with the sample entropy to decompose the original load series into a strong regular fluctuation component and a weak regular fluctuation component. Then, the gate recurrent unit neural network is used for point forecasting of the strong regular fluctuation component, and the support vector quantile regression model is used for interval forecasting of the weak regular fluctuation component, and the results are accumulated to obtain the final forecasting intervals. Finally, experiments were conducted using electricity load data from two regional electricity grids in Shaanxi Province, China. The results show that combining the idea of point interval, point forecasting, and interval forecasting for components with different fluctuation regularity can effectively reduce the forecasting interval width while having high accuracy. The proposed model has higher forecasting accuracy and smaller mean interval width at various confidence levels compared to the commonly used models.
Automatic Fed-Batch Cultivation Enhances Microbial Lipid Production from Volatile Fatty Acids
Authors: Shin Subin , Go Jae Hee , Moon Myounghoon , Park Gwon Woo
Published: February 2023

Organic waste is generated worldwide, and its disposal and recycling are becoming a challenge. Due to its high carbon content, however, it may be converted into valuable products. Carbon neutrality is essential, and unstable international oil prices stress the increasing importance of biofuels significantly. Volatile fatty acids (VFA) derived from organic waste can be converted to microbial lipids by oleaginous yeast using it as a carbon source. When VFA is consumed by oleaginous yeast, the pH of the medium rises; hence, acidic agents have to be added to the medium to maintain the broth’s pH. In this study, we enhanced microbial lipid productivity by automatic fed-batch cultivation using VFA as an acidic agent, and the modified cultivation showed 48.9% and 69.0% higher biomass and lipid productivity than manual multi-fed culture. At a VFA concentration of 5 g/L and pH 7.0, a lipid yield of 0.25 g/g alongside lipid productivity of 0.11 g/L/h was obtained from an automatic fed-batch system. Oleic acid accounted for the largest proportion of microbial lipids, and the fatty acid composition was suitable for biodiesel production.
Laccase Production from Local Biomass Using Solid State Fermentation
Authors: Hasan Shoaib , Anwar Zahid , Khalid Waseem , Afzal Fareed , Zafar Muddassar , Ali Usman , Refai Mohammed Y. , Afifi Mohamed , AL-Farga Ammar , Aljobair Moneera O.
Published: February 2023

The large family of enzymes, known as polyphenols oxidases, includes laccase. Due to the inclusion of a copper atom in their catalytic core, laccases are frequently referred to as multi-copper oxidases. Laccases are versatile enzymes that can catalyze the oxidation of a wide range of phenolic and non-phenolic substances. In the current study, a local strain of Aspergillus niger was used for solid-state fermentation to produce fungal laccase, as well as purify and optimize laccase. The enzyme profile, which was acquired using guaiacol to measure enzyme activity, showed that after five days of incubation, wheat straw provided the highest level of laccase activity, or 2.551 U/mL. A technique called response surface methodology (RSM) was used to examine the effects of various conditions on the production of enzymes. The RSM results demonstrated that after five days of incubation, the enzyme activity was at its highest at 45 °C, pH 5.5, and 30% moisture level, inoculated with 2 mL mycelium. Through ammonium sulphate precipitation and dialysis, the enzyme was purified. Additionally, column chromatography was used to further purify laccase. The next step was enzyme characterization to evaluate how temperature and pH affected enzyme activity. At 45 °C and pH 5.5, the isolated enzyme produced its highest level of activity. The findings of the current study showed that A. niger is capable of producing laccase in an economical and environmentally friendly way. Due to its unique oxidative and catalytic features, this enzyme has received a lot of attention recently.
Optimization of Wheat Straw Conversion into Microbial Lipids by Lipomyces tetrasporus DSM 70314 from Bench to Pilot Scale
Authors: Caporusso Antonio , De Bari Isabella , Giuliano Aristide , Liuzzi Federico , Albergo Roberto , Pietrafesa Rocchina , Siesto Gabriella , Romanelli Assunta , Braccio Giacobbe , Capece Angela
Published: February 2023

Microbial lipids are renewable platforms for several applications including biofuels, green chemicals, and nutraceuticals that can be produced from several residual carbon sources. Lignocellulosic biomasses are abundant raw materials for the production of second-generation sugars with conversion yields depending on the quality of the hydrolysates and the metabolic efficiency of the microorganisms. In the present work, wheat straw pre-treated by steam explosion and enzymatically hydrolysed was converted into microbial lipids by Lipomyces tetrasporus DSM 70314. The preliminary optimization of the enzymatic hydrolysis was performed at the bench scale through the response surface methodology (RSM). The fermentation medium and set-up were optimized in terms of the nitrogen (N) source and carbon-to-nitrogen (C/N) ratio yielding to the selection of soy flour as a N source and C/N ratio of 160. The bench scale settings were scaled-up and further optimized at the 10 L-scale and finally at the 50 L pilot scale bioreactor. Process optimization also included oxygen supply strategies. Under optimized conditions, a lipid concentration of 14.8 gL−1 was achieved corresponding to a 23.1% w/w lipid yield and 67.4% w/w lipid cell content. Oleic acid was the most abundant fatty acid with a percentage of 57%. The overall process mass balance was assessed for the production of biodiesel from wheat straw.
Evaluation of Fermentative Xylitol Production Potential of Adapted Strains of Meyerozyma caribbica and Candida tropicalis from Rice Straw Hemicellulosic Hydrolysate
Authors: Kaur Sundeep , Guleria Payal , Yadav Sudesh Kumar
Published: February 2023

Dilute acid hydrolysis of lignocellulosic biomass generates inhibitors in the hydrolysate which hamper yeast metabolism and the fermentation process. Therefore, understanding the effect of these compounds on the performance of microorganisms becomes essential to achieve improved product yields. In this study, the effect of acetic acid, furfural, and hydroxymethylfurfural was evaluated on yeast growth and fermentation efficiency. Various parameters for the pretreatment of rice straw, such as an acid catalyst, and its concentration and residence time, were optimized for the maximum liberation of sugars in the hydrolysate. Further, the yeast strains Candida tropicalis and Meyerozyma caribbica were adapted for the tolerance of inhibitors at higher concentrations. A comparative analysis was carried out using un-adapted and adapted strains of Candida tropicalis and Meyerozyma caribbica for xylitol production. The findings of this study revealed that sulfuric acid (1.25% v/v) at 121 ரC for 30 min can efficiently convert rice straw xylan to xylose, with the release of 16.07 g/L xylose in the hydrolysate. Further, the adaptation results showed an increase of 76.42% and 69.33% in xylose assimilation by C. tropicalis and M. caribbica, respectively. The xylitol production with the adapted C. tropicalis was increased by 7.54% to 28.03 g/L xylitol. However, the xylitol production with the adapted M. caribbica was increased by 8.33%, yielding 26.02 g/L xylitol in the non-detoxified hydrolysate when compared to the un-adapted strains. Repeated batch fermentation was carried out for seven batches, and xylitol was found to be efficiently produced by the yeasts during five successive batches without any significant loss in the xylitol yield. Moreover, the results suggest that M. caribbica is a promising microorganism for the transformation of rice straw-derived xylose to xylitol.
High Power Output Augmented Vertical Axis Wind Turbine
Authors: Salem Hayder , Mohammedredha Adel , Alawadhi Abdullah
Published: February 2023

Nowadays, wind energy is one of the most cost-effective and environmentally friendly energies in high demand due to shortages in fossil fuels and the necessity to reduce global carbon footprint. One of the main goals of wind turbine development is to increase the power output of the turbine either by increasing the turbine blade swept area or increasing the velocity of the wind. In this article, a proprietary augmentation system was introduced to increase the power output of vertical axis wind turbines (VAWT) by increasing the free stream velocity to more than two folds. The system comprises two identical airfoiled casings within which the turbine/turbines are seated. The results showed that the velocity slightly increases when decreasing the gap between the casing. It was also found that changing the angle of attack of the housing has more impact on the augmented airspeed. CFD technique was used to assess the velocity and flow of air around the system.
Hemodynamic Assessment of the Pathological Left Ventricle Function under Rest and Exercise Conditions
Authors: Korte Jana , Rauwolf Thomas , Thiel Jan-Niklas , Mitrasch Andreas , Groschopp Paulina , Neidlin Michael , Schmeißer Alexander , Braun-Dullaeus Rüdiger , Berg Philipp
Published: February 2023

Purpose: The analysis of pathological human left ventricular hemodynamics using high-resolved image-based blood flow simulations shows a major potential for examining mitral valve insufficiency (MI) under exercise conditions. Since capturing and simulating the patient-specific movement of the left ventricle (LV) during rest and exercise is challenging, this study aims to propose a workflow to analyze the hemodynamics within the pathologically moving LV. Methods: Patient-specific ultrasound (US) data of ten patients with MI in different stages were captured with three-dimensional real-time echocardiography. US measurements were performed while patients were resting and while doing handgrip exercise (2–4 min work). Patient-specific hemodynamic simulations were carried out based on the captured ventricular wall movement. Velocity and kinetic energy were analyzed for rest and exercise and for the different MI stages. Results: The results reveal a dependency of the kinetic energy over time in the ventricular volume curves. Concerning the comparison between rest and exercise, the left ventricular function reveals lower systolic kinetic energy under exercise (kinetic energy normalized by EDV; mean ± standard deviation: rest = 0.16 ± 0.14; exercise = 0.06 ± 0.05; p-value = 0.04). Comparing patients with non-limiting (MI I) and mild/moderate (MI II/III) MI, lower velocities (mean ± standard deviation: non-limiting = 0.10 ± 0.03; mild/moderate = 0.06 ± 0.02; p-value = 0.01) and lower diastolic kinetic energy (kinetic energy normalized by EDV; mean ± standard deviation: non-limiting = 0.45 ± 0.30; mild/moderate = 0.20 ± 0.19; p-value = 0.03) were found for the latter. Conclusion: With the proposed workflow, the hemodynamics within LVs with MI can be analyzed under rest and exercise. The results reveal the importance of the patient-specific wall movement when analyzing intraventricular hemodynamics. These findings can be further used within patient-specific simulations, based on varying the imaging and segmentation methods.
Kinetic Features of Cd and Zn Cathodic Formations in the Membrane Electrolysis Process
Authors: Serdiuk Vasyl , Pavlenko Ivan , Bolshanina Svitlana , Sklabinskyi Vsevolod , Włodarczak Sylwia , Krupińska Andżelika , Matuszak Magdalena , Bielecki Zdzisław , Ochowiak Marek
Published: February 2023

Chromate and dichromate solutions used for the activation and passivation of cadmium and zinc galvanic coatings of metal products are widely used due to their ability to form corrosion-protective films. Therefore, in this article, we examined the kinetic features of the cathodic deposition of Cd and Zn during membrane electrolysis. As a result of comprehensive experimental and theoretical studies, the features of Cd and Zn cathodic depositions were analyzed under different hydrodynamic conditions in a submembrane zone of an anolyte. Experimental physicochemical methods such as the experimental analysis of solutions, analytical modeling, and a statistical analysis were used during the research. A regression dependence for evaluating a reaction rate constant was assessed based on the least-square approximation of the proposed model. As a result, the peculiarities of the cathodic formations for Cd and Zn during the membrane electrolysis process were analyzed. The effect of mechanical mixing with different values of the Reynolds number on the deposition of Cd and Zn on a cathode was evaluated. A change in Cd2+ and Zn2+ ion concentrations was also considered during the research. Overall, the obtained results increased the Cd deposition rate by 2.2 times using an active hydrodynamic environment with the anolyte.
Natural Compounds in Plant-Based Food
Authors: Eisenreich Andreas , Schäfer Bernd
Published: February 2023

Plant-based foods include a wide range of products, such as fruits, vegetables, herbs and spices, as well as food products based on them, such as sauces, soups, or beverages [...]
Enhanced Rupture Force in a Cut-Dispersed Double-Network Hydrogel
Authors: Zhu Shilei , Yan Dongdong , Chen Lin , Wang Yan , Zhu Fengbo , Ye Yanan , Zheng Yong , Yu Wenwen , Zheng Qiang
Published: February 2023

The Kirigami approach is an effective way to realize controllable deformation of intelligent materials via introducing cuts into bulk materials. For materials ranging from ordinary stiff materials such as glass, ceramics, and metals to soft materials, including ordinary hydrogels and elastomers, all of them are all sensitive to the presence of cuts, which usually act as defects to deteriorate mechanical properties. Herein, we study the influence of the cuts on the mechanical properties by introducing “dispersed macro-scale cuts” into a model tough double network (DN) hydrogel (named D-cut gel), which consists of a rigid and brittle first network and a ductile stretchable second network. For comparison, DN gels with “continuous cuts” having the same number of interconnected cuts (named C-cut gel) were chosen. The fracture tests of D-cut gel and C-cut gel with different cut patterns were performed. The fracture observation revealed that crack blunting occurred at each cut tip, and a large wrinkle-like zone was formed where the wrinkles were parallel to the propagation direction of the cut. By utilizing homemade circular polarizing optical systems, we found that introducing dispersed cuts increases the rupture force by homogenizing the stress around the crack tip surrounding every cut, which reduces stress concentration in one certain cut. We believe this work reveals the fracture mechanism of tough soft materials with a kirigami cut structure, which should guide the design of advanced soft and tough materials along this line.
Origin of the Springback Effect in Ambient-Pressure-Dried Silica Aerogels: The Effect of Surface Silylation
Authors: Zemke Fabian , Gonthier Julien , Scoppola Ernesto , Simon Ulla , Bekheet Maged F. , Wagermaier Wolfgang , Gurlo Aleksander
Published: February 2023

Ambient pressure drying (APD) can prospectively reduce the costs of aerogel fabrication and processing. APD relies solely on preventing shrinkage or making it reversible. The latter, i.e., the aerogel re-expansion after drying (so-called springback effect—SBE), needs to be controlled for reproducible aerogel fabrication by APD. This can be achieved by an appropriate surface functionalization of aerogel materials (e.g., SiO2). This work addresses the fabrication of monolithic SiO2 aerogels and xerogels by APD. The effect of several silylation agents, i.e., trimethylchlorosilane, triethylchlorosilane, and hexamethyldisilazane on the SBE is studied in detail, applying several complementary experimental techniques, allowing the evaluation of the macroscopic and microscopic morphology as well as the composition of SiO2 aerogels. Here, we show that some physical properties, e.g., the bulk density, the macroscopic structure, and pore sizes/volumes, were significantly affected by the re-expansion. However, silylation did not necessarily lead to full re-expansion. Therefore, similarities in the molecular composition could not be equated to similarities in the SBE. The influences of steric hindrance and reactivity are discussed. The impact of silylation is crucial in tailoring the SBE and, as a result, the APD of monolithic aerogels.
Polymer Gels: Classification and Recent Developments in Biomedical Applications
Authors: Chelu Mariana , Musuc Adina Magdalena
Published: February 2023

Polymer gels are a valuable class of polymeric materials that have recently attracted significant interest due to the exceptional properties such as versatility, soft-structure, flexibility and stimuli-responsive, biodegradability, and biocompatibility. Based on their properties, polymer gels can be used in a wide range of applications: food industry, agriculture, biomedical, and biosensors. The utilization of polymer gels in different medical and industrial applications requires a better understanding of the formation process, the factors which affect the gel’s stability, and the structure-rheological properties relationship. The present review aims to give an overview of the polymer gels, the classification of polymer gels’ materials to highlight their important features, and the recent development in biomedical applications. Several perspectives on future advancement of polymer hydrogel are offered.
Regulatory Effect of Exogenous γ-Aminobutyric Acid on Respiratory Rate through the γ-Aminobutyric Acid Shunt in Malus baccata (L.) Borkh. Roots under Suboptimal Low Root-Zone Temperature
Authors: Lu Xiaochen , Dai Ping , Ma Huaiyu , Lyu Deguo
Published: February 2023

Malus baccata (L.) Borkh. is one of the most widely used rootstocks in the apple-producing region of Northern China. However, in the early growing season, apple roots are often subjected to suboptimal low root-zone temperatures. The regulatory effects of exogenous γ-aminobutyric acid (GABA) on both the γ-aminobutyric acid shunt (GABA shunt) and the respiratory activity of roots under suboptimal low root-zone temperatures remain unknown. To explore the physiological basis for GABA alleviation of low-temperature stress in M. baccata Borkh. roots, the following treatments were examined: suboptimal low root-zone temperature (potted parts of the seedlings were maintained at 5 ± 0.5 °C; L); suboptimal low root-zone temperature + GABA (LG); and suboptimal low root-zone temperature + vigabatrin (VGB; LV), which is a specific active inhibitor of γ-aminobutyric acid transaminase (GABA-T). Each treatment was matched with a control (18 °C/8 °C day/night; CK) for comparison. Our results showed that the L treatment reduced the root vitality, increased malondialdehyde (MDA) content, promoted the accumulation of GABA, activated the GABA shunt, and inhibited the total root respiration rate (VTotal) by decreasing the respiratory rates of Embden–Meyerhof pathway (VEMP) and tricarboxylic acid cycle (VTCAC). The LG treatment significantly increased the content of endogenous GABA, accelerated the metabolism of the GABA shunt, enhanced root respiratory activity by increasing VTotal, VEMP, VTCAC, and increased the cytochrome pathway respiratory rate (VCP), thus alleviating the damage of low root-zone temperature stress. Meanwhile, contrasting results were observed in the LV treatment. These findings revealed that exogenous GABA improved the tolerance of apple rootstocks to suboptimal low temperatures in early spring by regulating the GABA shunt and root respiratory activity.
Quantitative Estimation of Rainfall from Remote Sensing Data Using Machine Learning Regression Models
Authors: Mohia Yacine , Absi Rafik , Lazri Mourad , Labadi Karim , Ouallouche Fethi , Ameur Soltane
Published: February 2023

To estimate rainfall from remote sensing data, three machine learning-based regression models, K-Nearest Neighbors Regression (K-NNR), Support Vector Regression (SVR), and Random Forest Regression (RFR), were implemented using MSG (Meteosat Second Generation) satellite data. Daytime and nighttime data from a rain gauge are used for model training and validation. To optimize the results, the outputs of the three models are combined using the weighted average. The combination of the three models (hereafter called Com-RSK) markedly improved the predictions. Indeed, the MAE, MBE, RMSE and correlation coefficient went from 23.6 mm, 10.0 mm, 40.6 mm and 89% for the SVR to 20.7 mm, 5.5 mm, 37.4 mm, and 94% when the models were combined, respectively. The Com-RSK is also compared to a few methods using the classification in the estimation, such as the ECST Enhanced Convective Stratiform Technique (ECST), the MMultic technique, and the Convective/Stratiform Rain Area Delineation Technique (CS-RADT). The Com-RSK show superior performance compared to ECST, MMultic and CS-RADT methods.The Com-RSK is also compared to the two products of satellite estimates, namely CMORPH and CHIRPS. The results indicate that Com-RSK performs better than CMORPH and CHIRPS according to MBE, RMSE and CC (coefficient correlation). A comparison with three types of satellite precipitation estimation products, such as global product, regional product, and near real-time product, is performed. Overall, the methodology developed here shows almost the same results as regional product methods and exhibits better results than near real-time and global product methods.
Bio-Derived Furanic Compounds with Natural Metabolism: New Sustainable Possibilities for Selective Organic Synthesis
Authors: Romashov Leonid V. , Kucherov Fedor A. , Kozlov Kirill S. , Ananikov Valentine P.
Published: February 2023

Biomass-derived C6-furanic compounds have become the cornerstone of sustainable technologies. The key feature of this field of chemistry is the involvement of the natural process only in the first step, i.e., the production of biomass by photosynthesis. Biomass-to-HMF (5-hydroxymethylfurfural) conversion and further transformations are carried out externally with the involvement of processes with poor environmental factors (E-factors) and the generation of chemical wastes. Due to widespread interest, the chemical conversion of biomass to furanic platform chemicals and related transformations are thoroughly studied and well-reviewed in the current literature. In contrast, a novel opportunity is based on an alternative approach to consider the synthesis of C6-furanics inside living cells using natural metabolism, as well as further transformations to a variety of functionalized products. In the present article, we review naturally occurring substances containing C6-furanic cores and focus on the diversity of C6-furanic derivatives, occurrence, properties and synthesis. From the practical point of view, organic synthesis involving natural metabolism is advantageous in terms of sustainability (sunlight-driven as the only energy source) and green nature (no eco-persisted chemical wastes).
The “ON-OFF” Switching Response of Reactive Oxygen Species in Acute Normobaric Hypoxia: Preliminary Outcome
Authors: Mrakic-Sposta Simona , Gussoni Maristella , Marzorati Mauro , Porcelli Simone , Bosco Gerardo , Balestra Costantino , Montorsi Michela , Lafortuna Claudio , Vezzoli Alessandra
Published: February 2023

Exposure to acute normobaric hypoxia (NH) elicits reactive oxygen species (ROS) accumulation, whose production kinetics and oxidative damage were here investigated. Nine subjects were monitored while breathing an NH mixture (0.125 FIO2 in air, about 4100 m) and during recovery with room air. ROS production was assessed by Electron Paramagnetic Resonance in capillary blood. Total antioxidant capacity, lipid peroxidation (TBARS and 8-iso-PFG2α), protein oxidation (PC) and DNA oxidation (8-OH-dG) were measured in plasma and/or urine. The ROS production rate (μmol·min−1) was monitored (5, 15, 30, 60, 120, 240 and 300 min). A production peak (+50%) was reached at 4 h. The on-transient kinetics, exponentially fitted (t1/2 = 30 min r2 = 0.995), were ascribable to the low O2 tension transition and the mirror-like related SpO2 decrease: 15 min: −12%; 60 min: −18%. The exposure did not seem to affect the prooxidant/antioxidant balance. Significant increases in PC (+88%) and 8-OH-dG (+67%) at 4 h in TBARS (+33%) one hour after hypoxia offset were also observed. General malaise was described by most of the subjects. Under acute NH, ROS production and oxidative damage resulted in time and SpO2-dependent reversible phenomena. The experimental model could be suitable for evaluating the acclimatation level, a key element in the context of mountain rescues in relation to technical/medical workers who have not had enough time for acclimatization—as, for example, during helicopter flights.
Critical Assessment of the Prospects of Quorum-Quenching Therapy for Staphylococcus aureus Infection
Authors: Otto Michael
Published: February 2023

Staphylococcus aureus is an important pathogen that causes a high number of infections and is one of the leading causes of death in hospitalized patients. Widespread antibiotic resistance such as in methicillin-resistant S. aureus (MRSA) has prompted research into potential anti-virulence-targeted approaches. Targeting the S. aureus accessory gene regulator (Agr) quorum-sensing system, a master regulator of virulence, is the most frequently proposed anti-virulence strategy for S. aureus. While much effort has been put into the discovery and screening for Agr inhibitory compounds, in vivo analysis of their efficacy in animal infection models is still rare and reveals various shortcomings and problems. These include (i) an almost exclusive focus on topical skin infection models, (ii) technical problems that leave doubt as to whether observed in vivo effects are due to quorum-quenching, and (iii) the discovery of counterproductive biofilm-increasing effects. Furthermore, potentially because of the latter, invasive S. aureus infection is associated with Agr dysfunctionality. Altogether, the potential of Agr inhibitory drugs is nowadays seen with low enthusiasm given the failure to provide sufficient in vivo evidence for their potential after more than two decades since the initiation of such efforts. However, current Agr inhibition-based probiotic approaches may lead to a new application of Agr inhibition strategies in preventing S. aureus infections by targeting colonization or for otherwise difficult-to-treat skin infections such as atopic dermatitis.
Natural Killer Cell-Derived Extracellular Vesicles as a Promising Immunotherapeutic Strategy for Cancer: A Systematic Review
Authors: Chan Alvin Man Lung , Cheah Jin Min , Lokanathan Yogeswaran , Ng Min Hwei , Law Jia Xian
Published: February 2023

Cancer is the second leading contributor to global deaths caused by non-communicable diseases. The cancer cells are known to interact with the surrounding non-cancerous cells, including the immune cells and stromal cells, within the tumor microenvironment (TME) to modulate the tumor progression, metastasis and resistance. Currently, chemotherapy and radiotherapy are the standard treatments for cancers. However, these treatments cause a significant number of side effects, as they damage both the cancer cells and the actively dividing normal cells indiscriminately. Hence, a new generation of immunotherapy using natural killer (NK) cells, cytotoxic CD8+ T-lymphocytes or macrophages was developed to achieve tumor-specific targeting and circumvent the adverse effects. However, the progression of cell-based immunotherapy is hindered by the combined action of TME and TD-EVs, which render the cancer cells less immunogenic. Recently, there has been an increase in interest in using immune cell derivatives to treat cancers. One of the highly potential immune cell derivatives is the NK cell-derived EVs (NK-EVs). As an acellular product, NK-EVs are resistant to the influence of TME and TD-EVs, and can be designed for “off-the-shelf” use. In this systematic review, we examine the safety and efficacy of NK-EVs to treat various cancers in vitro and in vivo.
“Pesto” Mutation: Phenotypic and Genotypic Characteristics of Eight GCK/MODY Ligurian Patients
Authors: Salina Alessandro , Bassi Marta , Aloi Concetta , Strati Marina Francesca , Bocciardi Renata , d’Annunzio Giuseppe , Maghnie Mohamad , Minuto Nicola
Published: February 2023

Maturity Onset Diabetes of the Young (MODY) is a monogenic form of diabetes mellitus (DM) that accounts for around 2–5% of all types of diabetes. Autosomal dominant inheritance in pathogenic variations of 14 genes related to β-cell functions can lead to monogenic types of diabetes. In Italy, GCK/MODY is the most frequent form and it is caused by mutations of the glucokinase (GCK). Patients with GCK/MODY usually have stable mild fasting hyperglycaemia with mildly elevated HbA1c levels and rarely need pharmacological treatment. Molecular analysis of the GCK coding exons was carried out by Sanger sequencing in eight Italian patients. All the probands were found to be heterozygous carriers of a pathogenic gross insertion/deletion c.1279_1358delinsTTACA; p.Ser426_Ala454delinsLeuGln. It was previously described for the first time by our group in a large cohort of Italian GCK/MODY patients. The higher levels of HbA1c (6.57% vs. 6.1%), and the higher percentage of patients requiring insulin therapy (25% vs. 2%) compared to the previously studied Italian patients with GCK/MODY, suggest that the mutation discovered could be responsible for a clinically worse form of GCK/MODY. Moreover, as all the patients carrying this variant share an origin from the same geographic area (Liguria), we postulate a possible founder effect and we propose to name it the “pesto” mutation.