bluuebunny/crossref_metadata_2025 huggingface dataset:
Insomnia and Personality—A Network Approach
Authors: Dekker Kim , Blanken Tessa , Van Someren Eus
Published: March 2017

Studies on personality traits and insomnia have remained inconclusive about which traits show the most direct associations with insomnia severity. It has moreover hardly been explored how traits relate to specific characteristics of insomnia. We here used network analysis in a large sample (N = 2089) to obtain an integrated view on the associations of personality traits with both overall insomnia severity and different insomnia characteristics, while distinguishing direct from indirect associations. We first estimated a network describing the associations among the five factor model personality traits and overall insomnia severity. Overall insomnia severity was associated with neuroticism, agreeableness, and openness. Subsequently, we estimated a separate network describing the associations among the personality traits and each of the seven individual items of the Insomnia Severity Index. This revealed relatively separate clusters of daytime and nocturnal insomnia complaints, that both contributed to dissatisfaction with sleep, and were both most directly associated with neuroticism and conscientiousness. The approach revealed the strongest direct associations between personality traits and the severity of different insomnia characteristics and overall insomnia severity. Differentiating them from indirect associations identified the targets for improving Cognitive Behavioral Therapy for insomnia with the highest probability of effectively changing the network of associated complaints.
Investigating Philosophies Underpinning Dietetic Private Practice
Authors: Harper Claudia , Maher Judith
Published: March 2017

There is limited theory or knowledge regarding dietitians’ practice philosophies and how these philosophies are generated and incorporated into their professional practices. For the purposes of this study, a conceptual framework will explain and define the ‘philosophies’ as three different types of knowledge; episteme, techne, and phronesis. This study aimed to develop an explanatory theory of how dietitians in private practice source, utilise, and integrate practice philosophies. A grounded theory qualitative methodology was used to inform the sampling strategy, data collection, and analytical processes. Semi-structured interviews with dietitians in private practice were undertaken and data were collected and analysed concurrently. The results show that dietitians form collaborative relationships with their clients, in order to nurture change over time. They use intrinsic and intertwined forms of episteme, techne, and phronesis, which allow them to respond both practically and sensitively to their clients’ needs. The learning and integration of these forms of knowledge are situated in their own practice experience. Dietitians adapt through experience, feedback, and reflection. This study highlights that private practice offers a unique context in which dietitians deal with complex issues, by utilising and adapting their philosophies.
The Acoustics of the Double Elliptical Vault of the Royal Palace of Caserta (Italy)
Authors: Berardi Umberto , Iannace Gino , Trematerra Amelia
Published: March 2017

This work investigates the acoustic characteristics of the double elliptical vault, which overlooks the Grand Staircase of the Royal Palace of Caserta (Italy). The Royal Palace was built by the architect Luigi Vanvitelli in the Seventeenth Century and it is the largest royal building in Italy. The double elliptical vault presents a great scenography effect. Inside the vault, on the planking level, musicians used to play for the king and his guests when the royal procession, going up the grand staircase, entered the royal apartments, creating astonishment among the guests who heard the music without understanding from where it was coming. Since the musicians were inside the vault, the long reverberation made the listeners perceive the vault to be enveloped by the music. To investigate this effect, the acoustic characteristics of the double vault were measured, putting the sound source on the planking level of the vault, while the microphones were put along the staircase and in the vestibule towards the royal apartments. Finally, the spatial distribution of several acoustic parameters is evaluated also using architectural acoustic simulations.
The Influence of the Antarctic Oscillation (AAO) on Cold Waves and Occurrence of Frosts in the State of Santa Catarina, Brazil
Authors: Alves Maikon , Brito Silveira Rafael , Boligon Minuzzi Rosandro , Elvino Franke Alberto
Published: March 2017

This paper examines the relationship between the Antarctic Oscillation (AAO), cold waves and occurrence of frosts in the state of Santa Catarina, Brazil, during the winter quarter. Research on this topic can assist different spheres of society, such as public health and agriculture, since cold waves can influence and/or aggravate health problems and frosts can inflict economic losses especially in the agricultural sector. For the purpose of this paper, cold wave is considered as the event in which the daily average surface air temperature was at least two standard deviations below the average value of the series on the day and for two consecutive days or more. The data on the average air temperature and frost occurrences are provided by the Company of Agricultural Research and Rural Extension of Santa Catarina/Center for Environmental Information and Hydrometeorology (EPAGRI/CIRAM). The AAO was subjected to statistical analysis using significance tests for the averages (Student’s t-test) and variances (F-test) with a significance level of α = 5%. The results show that cold waves are unevenly distributed in the agroecological zones of Santa Catarina. It is found that the AAO is associated with the occurrence of frosts (in the agroecological zones represented by the municipalities of Itajaí and São José) in the state of Santa Catarina.
A Pilot Study into the Association between Oral Health Status and Human Papillomavirus—16 Infection
Authors: Sun Charles , Bennett Nigel , Tran Peter , Tang Kai , Lim Yenkai , Frazer Ian , Samaranayake Lakshman , Punyadeera Chamindie
Published: March 2017

Background: Over the next 20 years, oropharyngeal cancers (OPC) will represent the majority of head and neck cancers (HNCs) in the United States. It is estimated that human papillomavirus (HPV) may account for as much as 70% to 80% of OPCs in North America and in certain parts of Europe. It is hence crucial to understand the disease risk factors and natural history of oral HPV infections. We hypothesized that poor oral health (by measures such as poor oral hygiene and periodontal disease) leads to a higher degree of oral HPV-16 infections within a patient cohort from a dental school clinic. This study aims to test this hypothesis and gauge possible disease associations before larger scale studies. Subjects and Methods: 223 participants were recruited in this study from the University of Queensland Dental School clinic. Clinical oral health parameters (such as oral hygiene measures and periodontal disease measurements) have been examined and determined by dental professionals. We have collected oral rinse samples from these volunteers. Results: 10 (4.5%) out of 223 participants were found to have HPV-16 DNA in their oral rinse samples using NB2 endpoint PCR and Sanger sequencing. Within the HPV-16 DNA positive subjects, 7 (70%) and 3 (30%) were associated with poor oral hygiene and periodontal disease, respectively. Conclusion: Our results show a trend towards a positive correlation between oral HPV-16 infection and poor clinical oral health status.
Subtypes of Ovarian Cancer and Ovarian Cancer Screening
Authors: Koshiyama Masafumi , Matsumura Noriomi , Konishi Ikuo
Published: March 2017

Ovarian cancer is the foremost cause of gynecological cancer death in the developed world, as it is usually diagnosed at an advanced stage. In this paper we discuss current issues, the efficacy and problems associated with ovarian cancer screening, and compare the characteristics of ovarian cancer subtypes. There are two types of ovarian cancer: Type I carcinomas, which are slow-growing, indolent neoplasms thought to arise from a precursor lesion, which are relatively common in Asia; and Type II carcinomas, which are clinically aggressive neoplasms that can develop de novo from serous tubal intraepithelial carcinomas (STIC) and/or ovarian surface epithelium and are common in Europe and the USA. One of the most famous studies on the subject reported that annual screening using CA125/transvaginal sonography (TVS) did not reduce the ovarian cancer mortality rate in the USA. In contrast, a recent study in the UK showed an overall average mortality reduction of 20% in the screening group. Another two studies further reported that the screening was associated with decreased stage at detection. Theoretically, annual screening using CA125/TVS could easily detect precursor lesions and could be more effective in Asia than in Europe and the USA. The detection of Type II ovarian carcinoma at an early stage remains an unresolved issue. The resolving power of CA125 or TVS screening alone is unlikely to be successful at resolving STICs. Biomarkers for the early detection of Type II carcinomas such as STICs need to be developed.
The Spectrum of Neurological Manifestations Associated with Gaucher Disease
Authors: Roshan Lal Tamanna , Sidransky Ellen
Published: March 2017

Gaucher disease, the most common lysosomal storage disorder, is due to a deficiency in the enzyme glucocerebrosidase. This leads to the accumulation of its normal substrate, glucocerebroside, in tissue macrophages, affecting the hematological, visceral, bone and neurologic systems. Gaucher disease is classified into three broad phenotypes based upon the presence or absence of neurological involvement: type 1 (non-neuronopathic), type 2 (acute neuronopathic), and type 3 (subacute neuronopathic). Phenotypically, there is a wide spectrum of visceral and neurological manifestations. Enzyme replacement is effective in managing the visceral disease; however, treating the neurological manifestations has proved to be more challenging. This review discusses the various neurological manifestations encountered in Gaucher disease, and provides a brief overview regarding the treatment and ongoing research challenges.
Highly Sensitive and Selective VOC Sensor Systems Based on Semiconductor Gas Sensors: How to?
Authors: Schütze Andreas , Baur Tobias , Leidinger Martin , Reimringer Wolfhard , Jung Ralf , Conrad Thorsten , Sauerwald Tilman
Published: March 2017

Monitoring of volatile organic compounds (VOCs) is of increasing importance in many application fields such as environmental monitoring, indoor air quality, industrial safety, fire detection, and health applications. The challenges in all of these applications are the wide variety and low concentrations of target molecules combined with the complex matrix containing many inorganic and organic interferents. This paper will give an overview over the application fields and address the requirements, pitfalls, and possible solutions for using low-cost sensor systems for VOC monitoring. The focus lies on highly sensitive metal oxide semiconductor gas sensors, which show very high sensitivity, but normally lack selectivity required for targeting relevant VOC monitoring applications. In addition to providing an overview of methods to increase the selectivity, especially virtual multisensors achieved with dynamic operation, and boost the sensitivity further via novel pro-concentrator concepts, we will also address the requirement for high-performance gas test systems, advanced solutions for operating and read-out electronic, and, finally, a cost-efficient factory and on-site calibration. The various methods will be primarily discussed in the context of requirements for monitoring of indoor air quality, but can equally be applied for environmental monitoring and other fields.
Domain-Specific Self-Reported and Objectively Measured Physical Activity in Children
Authors: Sprengeler Ole , Wirsik Norman , Hebestreit Antje , Herrmann Diana , Ahrens Wolfgang
Published: March 2017

Little is known about the extent that different domains contribute to total sedentary (SED), light (LPA) and moderate-to-vigorous physical activity (MVPA). We aimed to identify domain-specific physical activity (PA) patterns in school-aged children who were assessed by questionnaire and accelerometry. For the study, 298 German school children and adolescents aged 6–17 years wore an accelerometer for one week and completed a PA recall-questionnaire for the same period. Spearman coefficients (r) were used to evaluate the agreement between self-reported and objectively measured PA in five domains (transport, school hours, physical education, leisure-time, organized sports activities). School hours mainly contributed to the total objectively measured SED, LPA and MVPA (55%, 53% and 46%, respectively), whilst sports activities contributed only 24% to total MVPA. Compared to accelerometry, the proportion of self-reported LPA and MVPA during school hours was substantially underestimated but overestimated during leisure-time. The agreement of self-reported and objectively measured PA was low for total LPA (r = 0.09, 95% CI (confidence interval): −0.03–0.20) and total MVPA (r = 0.21, 95% CI: 0.10–0.32), while moderate agreement was only found for total SED (r = 0.44, 95% CI: 0.34–0.53), LPA during transport (r = 0.59; 95% CI: 0.49–0.67) and MVPA during organized sports activities (r = 0.54; 95% CI: 0.38–0.67). Since school hours mainly contribute to total SED, LPA and MVPA and self-reported LPA and MVPA during school were importantly underestimated compared to objectively measured LPA and MVPA, the application of objective measurements is compulsory to characterize the entire activity pattern of school-aged children.
Oil Price Uncertainty, Transport Fuel Demand and Public Health
Authors: He Ling-Yun , Yang Sheng , Chang Dongfeng
Published: March 2017

Based on the panel data of 306 cities in China from 2002 to 2012, this paper investigates China’s road transport fuel (i.e., gasoline and diesel) demand system by using the Almost Ideal Demand System (AIDS) and the Quadratic AIDS (QUAIDS) models. The results indicate that own-priceelasticitiesfordifferentvehiclecategoriesrangefrom−1.215to−0.459(byAIDS)andfrom −1.399 to−0.369 (by QUAIDS). Then, this study estimates the air pollution emissions (CO, NOx and PM2.5) and public health damages from the road transport sector under different oil price shocks. Compared to the base year 2012, results show that a fuel price rise of 30% can avoid 1,147,270 tonnes of pollution emissions; besides, premature deaths and economic losses decrease by 16,149 cases and 13,817.953 million RMB yuan respectively; while based on the non-linear health effect model, the premature deaths and total economic losses decrease by 15,534 and 13,291.4 million RMB yuan respectively. Our study combines the fuel demand and health evaluation models and is the ﬁrst attempt to address how oil price changes inﬂuence public health through the fuel demand system in China. Given its serious air pollution emission and substantial health damages, this paper provides important insights for policy makers in terms of persistent increasing in fuel consumption and the associated health and economic losses.
Research and Application of an Air Quality Early Warning System Based on a Modified Least Squares Support Vector Machine and a Cloud Model
Authors: Wang Jianzhou , Niu Tong , Wang Rui
Published: March 2017

The worsening atmospheric pollution increases the necessity of air quality early warning systems (EWSs). Despite the fact that a massive amount of investigation about EWS in theory and practicality has been conducted by numerous researchers, studies concerning the quantification of uncertain information and comprehensive evaluation are still lacking, which impedes further development in the area. In this paper, firstly a comprehensive warning system is proposed, which consists of two vital indispensable modules, namely effective forecasting and scientific evaluation, respectively. For the forecasting module, a novel hybrid model combining the theory of data preprocessing and numerical optimization is first developed to implement effective forecasting for air pollutant concentration. Especially, in order to further enhance the accuracy and robustness of the warning system, interval forecasting is implemented to quantify the uncertainties generated by forecasts, which can provide significant risk signals by using point forecasting for decision-makers. For the evaluation module, a cloud model, based on probability and fuzzy set theory, is developed to perform comprehensive evaluations of air quality, which can realize the transformation between qualitative concept and quantitative data. To verify the effectiveness and efficiency of the warning system, extensive simulations based on air pollutants data from Dalian in China were effectively implemented, which illustrate that the warning system is not only remarkably high-performance, but also widely applicable.
Risk Estimates and Risk Factors Related to Psychiatric Inpatient Suicide—An Overview
Authors: Madsen Trine , Erlangsen Annette , Nordentoft Merete
Published: March 2017

People with mental illness have an increased risk of suicide. The aim of this paper is to provide an overview of suicide risk estimates among psychiatric inpatients based on the body of evidence found in scientific peer-reviewed literature; primarily focusing on the relative risks, rates, time trends, and socio-demographic and clinical risk factors of suicide in psychiatric inpatients. Psychiatric inpatients have a very high risk of suicide relative to the background population, but it remains challenging for clinicians to identify those patients that are most likely to die from suicide during admission. Most studies are based on low power, thus compromising quality and generalisability. The few studies with sufficient statistical power mainly identified non-modifiable risk predictors such as male gender, diagnosis, or recent deliberate self-harm. Also, the predictive value of these predictors is low. It would be of great benefit if future studies would be based on large samples while focusing on modifiable predictors over the course of an admission, such as hopelessness, depressive symptoms, and family/social situations. This would improve our chances of developing better risk assessment tools.
Japanese Mutual Funds before and after the Crisis Outburst: A Style- and Performance-Analysis
Authors: Papadamou Stephanos , Kyriazis Nikolaos , Mermigka Lydia
Published: March 2017

This paper investigates how mutual funds performed in Japan before and after the 2008 outburst of the global financial crisis, that is during the extension of an extraordinary unconventional monetary policy by the Bank of Japan. Style and performance analyses are employed in order to investigate whether active or passive management has been affected by unconventional times and to what extent. Evidence indicates that in four out of eight funds, asset selection presents a significant contribution to returns. The Selection Sharpe Ratios for sectoral and style analyses exhibit positive values added per unit of risk due to active management for the majority of our funds in the pre-Lehman default period. Nevertheless, none of them presents statistical significance according to the t-statistic. Moreover, over the post-Lehman default, only two out of eight funds achieved lower volatility levels and higher returns due to active management. A style drift to big capitalization stocks with low values of book to market ratio is to be held responsible for the outperformance. Overall, our findings imply that active management in a monetary easing environment does not add significant value to the mutual fund performance.
Lactoferrin: Balancing Ups and Downs of Inflammation Due to Microbial Infections
Authors: Drago-Serrano Maria , Campos-Rodríguez Rafael , Carrero Julio , De la Garza Mireya
Published: March 2017

Lactoferrin (Lf) is a glycoprotein of the primary innate immune-defense system of mammals present in milk and other mucosal secretions. This protein of the transferrin family has broad antimicrobial properties by depriving pathogens from iron, or disrupting their plasma membranes through its highly cationic charge. Noteworthy, Lf also exhibits immunomodulatory activities performing up- and down-regulation of innate and adaptive immune cells, contributing to the homeostasis in mucosal surfaces exposed to myriad of microbial agents, such as the gastrointestinal and respiratory tracts. Although the inflammatory process is essential for the control of invasive infectious agents, the development of an exacerbated or chronic inflammation results in tissue damage with life-threatening consequences. In this review, we highlight recent findings in in vitro and in vivo models of the gut, lung, oral cavity, mammary gland, and liver infections that provide experimental evidence supporting the therapeutic role of human and bovine Lf in promoting some parameters of inflammation and protecting against the deleterious effects of bacterial, viral, fungal and protozoan-associated inflammation. Thus, this new knowledge of Lf immunomodulation paves the way to more effective design of treatments that include native or synthetic Lf derivatives, which may be useful to reduce immune-mediated tissue damage in infectious diseases.
The PRR11-SKA2 Bidirectional Transcription Unit Is Negatively Regulated by p53 through NF-Y in Lung Cancer Cells
Authors: Wang Yitao , Weng Huali , Zhang Ying , Long Yinjiang , Li Yi , Niu Yulong , Song Fangzhou , Bu Youquan
Published: March 2017

We previously identified proline-rich protein 11 (PRR11) as a novel cancer-related gene that is implicated in the regulation of cell cycle and tumorigenesis. Our recent study demonstrated that PRR11 and its adjacent gene, kinetochore associated 2 (SKA2), constitute a classic head-to-head gene pair that is coordinately regulated by nuclear factor Y (NF-Y). In the present study, we further show that the PRR11-SKA2 bidirectional transcription unit is an indirect target of the tumor suppressor p53. A luciferase reporter assay revealed that overexpression of wild type p53, but not mutant p53, significantly represses the basal activity and NF-Y mediated transactivation of the PRR11-SKA2 bidirectional promoter. Deletion and mutation analysis of the PRR11-SKA2 promoter revealed that p53-mediated PRR11-SKA2 repression is dependent on the presence of functional NF-Y binding sites. Furthermore, a co-immunoprecipitation assay revealed that p53 associates with NF-Y in lung cancer cells, and a chromatin immunoprecipitation assay showed that p53 represses PRR11-SKA2 transcription by reducing the binding amount of NF-Y in the PRR11-SKA2 promoter region. Consistently, the ability of p53 to downregulate PRR11-SKA2 transcription was significantly attenuated upon siRNA-mediated depletion of nuclear factor Y subunit beta (NF-YB). Notably, lung cancer patients with lower expression of either PRR11 or SKA2 along with wild type p53 exhibited the best overall survival compared with others with p53 mutation and/or higher expression of either PRR11 or SKA2. Taken together, our results demonstrate that p53 negatively regulates the expression of the PRR11-SKA2 bidirectional transcription unit through NF-Y, suggesting that the inability to repress the PRR11-SKA2 bidirectional transcription unit after loss of p53 might contribute to tumorigenesis.
Evaluating Complex Mixtures in the Zebrafish Embryo by Reconstituting Field Water Samples: A Metal Pollution Case Study
Authors: Michiels Ellen , Vergauwen Lucia , Hagenaars An , Fransen Erik , Dongen Stefan , Van Cruchten Steven , Bervoets Lieven , Knapen Dries
Published: March 2017

Accurately assessing the toxicity of complex, environmentally relevant mixtures remains an important challenge in ecotoxicology. The goal was to identify biological effects after exposure to environmental water samples and to determine whether the observed effects could be explained by the waterborne metal mixture found in the samples. Zebrafish embryos were exposed to water samples of five different sites originating from two Flemish (Mol and Olen, Belgium) metal contaminated streams: “Scheppelijke Nete” (SN) and “Kneutersloop” (K), and a ditch (D), which is the contamination source of SN. Trace metal concentrations, and Na, K, Mg and Ca concentrations were measured using ICP-MS and were used to reconstitute site-specific water samples. We assessed whether the effects that were observed after exposure to environmental samples could be explained by metal mixture toxicity under standardized laboratory conditions. Exposure to “D” or “reconstituted D” water caused 100% mortality. SN and reconstituted SN water caused similar effects on hatching, swim bladder inflation, growth and swimming activity. A canonical discriminant analysis confirmed a high similarity between both exposure scenarios, indicating that the observed toxicity was indeed primarily caused by metals. The applied workflow could be a valuable approach to evaluate mixture toxicity that limits time and costs while maintaining biological relevance.
Portal Vein Embolization: Impact of Chemotherapy and Genetic Mutations
Authors: Deipolyi Amy , Zhang Yu , Khademhosseini Ali , Naidu Sailendra , Borad Mitesh , Sahin Burcu , Mathur Amit , Oklu Rahmi
Published: March 2017

We characterized the effect of systemic therapy given after portal vein embolization (PVE) and before hepatectomy on hepatic tumor and functional liver remnant (FLR) volumes. All 76 patients who underwent right PVE from 2002–2016 were retrospectively studied. Etiologies included colorectal cancer (n = 44), hepatocellular carcinoma (n = 17), cholangiocarcinoma (n = 10), and other metastases (n = 5). Imaging before and after PVE was assessed. Chart review revealed systemic therapy administration, SNaPshot genetic profiling, and comorbidities. Nine patients received systemic therapy; 67 did not. Tumor volume increased 28% in patients who did not receive and decreased −24% in patients who did receive systemic therapy (p = 0.026), with no difference in FLR growth (28% vs. 34%; p = 0.645). Among 30 patients with genetic profiling, 15 were wild type and 15 had mutations. Mutations were an independent predictor of tumor growth (p = 0.049), but did not impact FLR growth (32% vs. 28%; p = 0.93). Neither cirrhosis, hepatic steatosis, nor diabetes impacted changes in tumor or FLR volume (p > 0.20). Systemic therapy administered after PVE before hepatic lobectomy had no effect on FLR growth; however, it was associated with decreasing tumor volumes. Continuing systemic therapy until hepatectomy may be warranted, particularly in patients with genetic mutations.
Characterization of Blue Mold Penicillium Species Isolated from Stored Fruits Using Multiple Highly Conserved Loci
Authors: Yin Guohua , Zhang Yuliang , Pennerman Kayla , Wu Guangxi , Hua Sui , Yu Jiujiang , Jurick Wayne , Guo Anping , Bennett Joan
Published: March 2017

Penicillium is a large genus of common molds with over 400 described species; however, identification of individual species is difficult, including for those species that cause postharvest rots. In this study, blue rot fungi from stored apples and pears were isolated from a variety of hosts, locations, and years. Based on morphological and cultural characteristics and partial amplification of the β-tubulin locus, the isolates were provisionally identified as several different species of Penicillium. These isolates were investigated further using a suite of molecular DNA markers and compared to sequences of the ex-type for cognate species in GenBank, and were identified as P. expansum (3 isolates), P. solitum (3 isolates), P. carneum (1 isolate), and P. paneum (1 isolate). Three of the markers we used (ITS, internal transcribed spacer rDNA sequence; benA, β-tubulin; CaM, calmodulin) were suitable for distinguishing most of our isolates from one another at the species level. In contrast, we were unable to amplify RPB2 sequences from four of the isolates. Comparison of our sequences with cognate sequences in GenBank from isolates with the same species names did not always give coherent data, reinforcing earlier studies that have shown large intraspecific variability in many Penicillium species, as well as possible errors in some sequence data deposited in GenBank.
Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity
Authors: Da Costa Elisabete , Melo Tânia , Moreira Ana , Bernardo Carina , Helguero Luisa , Ferreira Isabel , Cruz Maria , Rego Andreia , Domingues Pedro , Calado Ricardo , Abreu Maria , Domingues Maria
Published: March 2017

The lipidome of the red seaweed Gracilaria sp., cultivated on land-based integrated multitrophic aquaculture (IMTA) system, was assessed for the first time using hydrophilic interaction liquid chromatography-mass spectrometry and tandem mass spectrometry (HILIC–MS and MS/MS). One hundred and forty-seven molecular species were identified in the lipidome of the Gracilaria genus and distributed between the glycolipids classes monogalactosyl diacylglyceride (MGDG), digalactosyl diacylglyceride (DGDG), sulfoquinovosyl monoacylglyceride (SQMG), sulfoquinovosyl diacylglyceride (SQDG), the phospholipids phosphatidylcholine (PC), lyso-PC, phosphatidylglycerol (PG), lyso-PG, phosphatidylinositol (PI), phosphatidylethanolamine (PE), phosphatic acid (PA), inositolphosphoceramide (IPC), and betaine lipids monoacylglyceryl- and diacylglyceryl-N,N,N-trimethyl homoserine (MGTS and DGTS). Antiproliferative and anti-inflammatory effects promoted by lipid extract of Gracilaria sp. were evaluated by monitoring cell viability in human cancer lines and by using murine macrophages, respectively. The lipid extract decreased cell viability of human T-47D breast cancer cells and of 5637 human bladder cancer cells (estimated half-maximal inhibitory concentration (IC50) of 12.2 μg/mL and 12.9 μg/mL, respectively) and inhibited the production of nitric oxide (NO) evoked by the Toll-like receptor 4 agonist lipopolysaccharide (LPS) on the macrophage cell line RAW 264.7 (35% inhibition at a concentration of 100 μg/mL). These findings contribute to increase the ranking in the value-chain of Gracilaria sp. biomass cultivated under controlled conditions on IMTA systems.
Comparison of Hydrostatic Extrusion between Pressure-Load and Displacement-Load Models
Authors: Du Shengqiang , Zan Xiang , Li Ping , Luo Laima , Zhu Xiaoyong , Wu Yucheng
Published: March 2017

Two finite element analysis (FEA) models simulating hydrostatic extrusion (HE) are designed, one for the case under pressure load and another for the case under displacement load. Comparison is made of the equivalent stress distribution, stress state ratio distribution and extrusion pressure between the two models, which work at the same extrusion ratio (R) and the same die angle (2α). A uniform Von-Mises equivalent stress gradient distribution and stress state ratio gradient distribution are observed in the pressure-load model. A linear relationship is found between the extrusion pressure (P) and the logarithm of the extrusion ratio (lnR), and a parabolic relationship between P and 2α, in both models. The P-value under pressure load is smaller than that under displacement load, though at the same R and α, and the difference between the two pressures becomes larger as R and α grow.
Paper-Based Microfluidic Device with a Gold Nanosensor to Detect Arsenic Contamination of Groundwater in Bangladesh
Authors: Chowdury Mosfera , Walji Noosheen , Mahmud Md. , MacDonald Brendan
Published: March 2017

In this paper, we present a microfluidic paper-based analytical device (μPAD) with a gold nanosensor functionalized with α-lipoic acid and thioguanine (Au–TA–TG) to detect whether the arsenic level of groundwater from hand tubewells in Bangladesh is above or below the World Health Organization (WHO) guideline level of 10 μg/L. We analyzed the naturally occurring metals present in Bangladesh groundwater and assessed the interference with the gold nanosensor. A method was developed to prevent interference from alkaline metals found in Bangladesh groundwater (Ca, Mg, K and Na) by increasing the pH level on the μPADs to 12.1. Most of the heavy metals present in the groundwater (Ni, Mn, Cd, Pb, and Fe II) did not interfere with the μPAD arsenic tests; however, Fe III was found to interfere, which was also prevented by increasing the pH level on the μPADs to 12.1. The μPAD arsenic tests were tested with 24 groundwater samples collected from hand tubewells in three different districts in Bangladesh: Shirajganj, Manikganj, and Munshiganj, and the predictions for whether the arsenic levels were above or below the WHO guideline level agreed with the results obtained from laboratory testing. The μPAD arsenic test is the first paper-based test validated using Bangladesh groundwater samples and capable of detecting whether the arsenic level in groundwater is above or below the WHO guideline level of 10 μg/L, which is a step towards enabling the villagers who collect and consume the groundwater to test their own sources and make decisions about where to obtain the safest water.
High-Throughput Particle Manipulation Based on Hydrodynamic Effects in Microchannels
Authors: Liu Chao , Hu Guoqing
Published: March 2017

Microfluidic techniques are effective tools for precise manipulation of particles and cells, whose enrichment and separation is crucial for a wide range of applications in biology, medicine, and chemistry. Recently, lateral particle migration induced by the intrinsic hydrodynamic effects in microchannels, such as inertia and elasticity, has shown its promise for high-throughput and label-free particle manipulation. The particle migration can be engineered to realize the controllable focusing and separation of particles based on a difference in size. The widespread use of inertial and viscoelastic microfluidics depends on the understanding of hydrodynamic effects on particle motion. This review will summarize the progress in the fundamental mechanisms and key applications of inertial and viscoelastic particle manipulation.
Synthesis of Aluminum Complexes Bearing 8-Anilide-5,6,7-trihydroquinoline Ligands: Highly Active Catalyst Precursors for Ring-Opening Polymerization of Cyclic Esters
Authors: Liu Shaofeng , Zhang Jie , Zuo Weiwei , Zhang Wenjuan , Sun Wen-Hua , Ye Hongqi , Li Zhibo
Published: March 2017

The stoichiometric reactions of 8-(2,6-R1-4-R2-anilide)-5,6,7-trihydroquinoline (LH) with AlR3 (R = Me or Et) afforded the aluminum complexes LAlR2 (Al1–Al5,Al1: R1 = iPr, R2 = H, R = Me; Al2: R1 = Me, R2 = H, R = Me; Al3: R1 = H, R2 = H, R = Me; Al4: R1 = Me, R2 = Me, R = Me; Al5: R1 = Me, R2 = Me, R = Et) in high yields. All aluminum complexes were characterized by NMR spectroscopy and elemental analysis. The molecular structures of complexes Al4 and Al5 were determined by single-crystal X-ray diffractions and revealed a distorted tetrahedral geometry at aluminum. In the presence of BnOH, complexes Al1–Al5 efficiently initiated the ring-opening homopolymerization of ε-caprolactone (ε-CL) and rac-lactide (rac-LA), respectively, in a living/controlled manner.
Generating Topographic Map Data from Classification Results
Authors: Höhle Joachim
Published: March 2017

The use of classification results as topographic map data requires cartographic enhancement and checking of the geometric accuracy. Urban areas are of special interest. The conversion of the classification result into topographic map data of high thematic and geometric quality is subject of this contribution. After reviewing the existing literature on this topic, a methodology is presented. The extraction of point clouds belonging to line segments is solved by the Hough transform. The mathematics for deriving polygons of orthogonal, parallel and general line segments by least squares adjustment is presented. A unique solution for polylines, where the Hough parameters are optimized, is also given. By means of two data sets land cover maps of six classes were produced and then enhanced by the proposed method. The classification used the decision tree method applying a variety of attributes including object heights derived from imagery. The cartographic enhancement is carried out with two different levels of quality. The user’s accuracies for the classes “impervious surface” and “building” were above 85% in the “Level 1” map of Example 1. The geometric accuracy of building corners at the “Level 2” maps is assessed by means of reference data derived from ortho-images. The obtained root mean square errors (RMSE) of the generated coordinates (x, y) were RMSEx = 1.2 m and RMSEy = 0.7 m (Example 1) and RMSEx = 0.8 m and RMSEy = 1.0 m (Example 2) using 31 and 62 check points, respectively. All processing for Level 1 (raster data) could be carried out with a high degree of automation. Level 2 maps (vector data) were compiled for the classes “building” and “road and parking lot”. For urban areas with numerous classes and of large size, universal algorithms are necessary to produce vector data fully automatically. The recent progress in sensors and machine learning methods will support the generation of topographic map data of high thematic and geometric accuracy.
A Doppler Radar System for Sensing Physiological Parameters in Walking and Standing Positions
Authors: Pour Ebrahim Malikeh , Sarvi Majid , Yuce Mehmet
Published: March 2017

Doppler radar can be implemented for sensing physiological parameters wirelessly at a distance. Detecting respiration rate, an important human body parameter, is essential in a range of applications like emergency and military healthcare environments, and Doppler radar records actual chest motion. One challenge in using Doppler radar is being able to monitor several patients simultaneously and in different situations like standing, walking, or lying. This paper presents a complete transmitter-receiver Doppler radar system, which uses a 4 GHz continuous wave radar signal transmission and receiving system, to extract base-band data from a phase-shifted signal. This work reports experimental evaluations of the system for one and two subjects in various standing and walking positions. It provides a detailed signal analysis of various breathing rates of these two subjects simultaneously. These results will be useful in future medical monitoring applications.
Rediscovering Rural Territories by Means of Religious Route Planning
Authors: Balestrieri Mara , Congiu Tanja
Published: March 2017

Since ancient times, pilgrimages have been an important expression of faith because slow-paced traveling, physical effort, and a closer relationship with nature favor introspection. Protecting pilgrimage routes means transforming them into a medium to rediscover and guide landscape development while avoiding possible speculative drifts. Such an approach is particularly important in rural areas often relegated to a marginal role in planning strategies, but frequently traversed by these itineraries. This paper deals with pilgrimage routes in Sardinia (Italy) and their chance to become an integral part of composite territorial infrastructures for triggering regeneration processes in rural areas. First, we introduce the topic with reference to planning literature and various case studies. Then, we select four pilgrimage itineraries that extend through different parts of the island, and compare them using a set of indicators regarding landscape, accessibility, visibility, equipment, planning, and management. A composite index has been developed to classify paths with respect to their potential to become part of a system of greenways, providing facilities and benefits to surrounding areas. The analysis highlights the strengths and weaknesses of local realities. It underlines factors, such as intrinsic characteristics of territories, local management, and decision-making circumstances, which affect trail potentialities to activate greenway projects to trace a new development perspective for rural areas.
Detecting Sybil Attacks in Cloud Computing Environments Based on Fail‐Stop Signature
Authors: Lim JongBeom , Yu HeonChang , Gil Joon‐Min
Published: March 2017

Due to the loosely coupled property of cloud computing environments, no node has complete knowledge of the system. For this reason, detecting a Sybil attack in cloud computing environments is a non‐trivial task. In such a dynamic system, the use of algorithms based on tree or ring structures for collecting the global state of the system has unfortunate downsides, that is, the structure should be re‐constructed in the presence of node joining and leaving. In this paper, we propose an unstructured Sybil attack detection algorithm in cloud computing environments. Our proposed algorithm uses one‐to‐one communication primitives rather than broadcast primitives and, therefore, the message complexity can be reduced. In our algorithmic design, attacker nodes forging multiple identities are effectively detected by normal nodes with the fail‐stop signature scheme. We show that, regardless of the number of attacker nodes, our Sybil attack detection algorithm is able to reach consensus.
Cytotoxic and Inflammatory Potential of Air Samples from Occupational Settings with Exposure to Organic Dust
Authors: Viegas Susana , Caetano Liliana , Korkalainen Merja , Faria Tiago , Pacífico Cátia , Carolino Elisabete , Quintal Gomes Anita , Viegas Carla
Published: March 2017

Organic dust and related microbial exposures are the main inducers of several respiratory symptoms. Occupational exposure to organic dust is very common and has been reported in diverse settings. In vitro tests using relevant cell cultures can be very useful for characterizing the toxicity of complex mixtures present in the air of occupational environments such as organic dust. In this study, the cell viability and the inflammatory response, as measured by the production of pro-inflammatory cytokines tumor necrosis factor-α (TNFα) and interleukin-1 β (IL-1β), were determined in human macrophages derived from THP-1 monocytic cells. These cells were exposed to air samples from five occupational settings known to possess high levels of contamination of organic dust: poultry and swine feed industries, waste sorting, poultry production and slaughterhouses. Additionally, fungi and particle contamination of those settings was studied to better characterize the organic dust composition. All air samples collected from the assessed workplaces caused both cytotoxic and pro-inflammatory effects. The highest responses were observed in the feed industry, particularly in swine feed production. This study emphasizes the importance of measuring the organic dust/mixture effects in occupational settings and suggests that differences in the organic dust content may result in differences in health effects for exposed workers.
Assessing the Risk Factors Associated with Malaria in the Highlands of Ethiopia: What Do We Need to Know?
Authors: Vajda Élodie , Webb Cameron
Published: March 2017

Malaria has been Ethiopia’s predominant communicable disease for decades. Following the catastrophic malaria outbreak in 2003–2004, the Federal Ministry of Health (FMoH) took drastic public health actions to lower the burden of malaria. The FMoH achieved significant declines in malaria mortality and incidence, and recently declared its objective to achieve malaria elimination in low malaria transmission areas of Ethiopia by 2020. However, while the overall malaria prevalence has decreased, unpredictable outbreaks increasingly occur irregularly in regions previously considered “malaria-free”. Such outbreaks have disastrous consequences on populations of these regions as they have no immunity against malaria. The Amhara Region accounts for 31% of Ethiopia’s malaria burden and is targeted for malaria elimination by the FMoH. Amhara’s epidemiological surveillance system faces many challenges to detect in a timely manner the unpredictable and irregular malaria outbreaks that occur in areas of otherwise low transmission. Despite the evidence of a shift in malaria transmission patterns, Amhara’s malaria control interventions remain constrained to areas that are historically known to have stable malaria transmission. This paper discusses the influence of temperature and precipitation variability, entomological parameters, and human population mobility on malaria transmission patterns across the Amhara Region, and in particular, in areas of unstable transmission. We argue that malaria epidemiological surveillance systems can be improved by accounting for population movements in addition to environmental and entomological factors. However, to date, no study has statistically analyzed the interplay of population dynamics on environmental and entomological drivers of malaria transmission.
Non-Standard Hierarchies of the Runnings of the Spectral Index in Inﬂation
Authors: Longden Chris
Published: March 2017

Recent analyses of cosmic microwave background surveys have revealed hints that there may be a non-trivial running of the running of the spectral index. If future experiments were to conﬁrm these hints, it would prove a powerful discriminator of inﬂationary models, ruling out simple single ﬁeld models. We discuss how isocurvature perturbations in multi-ﬁeld models can be invoked to generate large runnings in a non-standard hierarchy, and ﬁnd that a minimal model capable of practically realising this would be a two-ﬁeld model with a non-canonical kinetic structure. We also consider alternative scenarios such as variable speed-of-light models and canonical quantum gravity effects and their implications for runnings of the spectral index.
A New Strategy to Reduce Influenza Escape: Detecting Therapeutic Targets Constituted of Invariance Groups
Authors: Lao Julie , Vanet Anne
Published: March 2017

The pathogenicity of the different flu species is a real public health problem worldwide. To combat this scourge, we established a method to detect drug targets, reducing the possibility of escape. Besides being able to attach a drug candidate, these targets should have the main characteristic of being part of an essential viral function. The invariance groups that are sets of residues bearing an essential function can be detected genetically. They consist of invariant and synthetic lethal residues (interdependent residues not varying or slightly varying when together). We analyzed an alignment of more than 10,000 hemagglutinin sequences of influenza to detect six invariance groups, close in space, and on the protein surface. In parallel we identified five potential pockets on the surface of hemagglutinin. By combining these results, three potential binding sites were determined that are composed of invariance groups located respectively in the vestigial esterase domain, in the bottom of the stem and in the fusion area. The latter target is constituted of residues involved in the spring-loaded mechanism, an essential step in the fusion process. We propose a model describing how this potential target could block the reorganization of the hemagglutinin HA2 secondary structure and prevent viral entry into the host cell.
The Economic Analysis of Water Use in the Water Framework Directive Based on the System of Environmental-Economic Accounting for Water: A Case Study of the Guadalquivir River Basin
Authors: Gutiérrez-Martín Carlos , Borrego-Marín María , Berbel Julio
Published: March 2017

This paper develops a methodology for the economic analysis of water use proposed by the Water Framework Directive (WFD) based on the System of Environmental-Economic Accounting for Water (SEEA-Water) standard tables. Our proposal satisfies the requirements for the economic characterization set out in Article 5 of the WFD. A case study in the Guadalquivir river basin shows a similar characterization in the baseline scenario to previous studies, including apparent water productivity. The main contribution of our research, however, is the proposal of a methodology that would enhance comparability and knowledge-sharing between regions, countries, and sectors both in the European Union and worldwide.