bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Tobacco Consumption and Anxiety or Depression during Pregnancy on Maternal and Neonatal Health
Authors: Pereira Beatriz , Figueiredo Bárbara , Pinto Tiago Miguel , Míguez M. Carmen
Published: November 2020

This study analyzed the possible interaction effects between tobacco consumption and anxiety or depression during pregnancy on maternal and neonatal health. We recruited a sample of 807 pregnant Spanish women from public healthcare services. Women completed a questionnaire on sociodemographic variables, health status and tobacco consumption (continuous, quitting or no consumption) in the first and third trimester of pregnancy and at 2 months postpartum, and self-reported measures of anxiety and depression in the first trimester. Abstinence of tobacco consumption was verified through biochemical measurements. Interaction effects between tobacco consumption and anxiety were found for delivery (p < 0.001), neonatal health complications (p = 0.026) and gestational age at birth (p = 0.029). Interaction effects between tobacco consumption and depression were found for pregnancy (p = 0.032), delivery complications (p < 0.001) and weeks of gestation at birth (p = 0.031). This study suggests that there are different kinds of interaction effects between tobacco consumption and anxiety or depression. Smokers with high anxiety presented more delivery complications compared to quitters and non-smokers with high anxiety. There is a cumulative effect of anxiety on the effects of tobacco consumption on maternal health. The results highlighted the beneficial impact of quitting smoking during pregnancy to reduce the risk of suffering anxiety, depression and health complications.
Green, Brown, and Gray: Associations between Different Measurements of Land Patterns and Depression among Nursing Students in El Paso, Texas
Authors: Nazif-Munoz José Ignacio , Cedeno Laurent José Guillermo , Browning Matthew , Spengler John , Olvera Álvarez Héctor A.
Published: November 2020

Background: While greenness has been associated with lower depression, the generalizability of this association in arid landscapes remains undetermined. We assessed the association between depression and residential greenness, but also brownness and grayness among nursing students living in El Paso, Texas (the Chihuahuan desert). Methods: Depression was measured with the Patient Health Questionnaire-9 scale and greenness with the normalized difference vegetation index across three buffer sizes (i.e., 250, 500, and 1000 m). Using data from the National Land Cover Database, two additional measures of land patterns were analyzed: grayness and brownness. Structural equation models were used to assess the relationships of these land patterns to depression and quantify the indirect effects of peer alienation. Results: After adjusting for individual characteristics, at buffers 250 m, greenness was not associated with a decrease in the Incidence Rate Ratios (IRR) of depression (IRR, 0.51; 95% CI, 0.12–2.10); however, grayness and brownness were respectively associated with increases by 64% (IRR, 1.64; 95% CI, 1.07–2.52) and decreases by 35% (IRR, 0.65; 95% CI, 0.42–0.99). At buffer 250 m, peer alienation explained 17.43% (95% CI, −1.79–36.66) of the association between depression and brownness, suggesting a pathway to depression. Conclusions: We did not observe an association between depression and residential greenness in El Paso, Texas. However, we did observe a protective association between brownness and depression and an adverse association with grayness. These results have theoretical implications as they were based on commonly used frameworks in this literature, and adverse association of brownness (and the lack of greenness) and depression was expected.
Racial/Ethnic Discrimination and Intimate Partner Violence Perpetration in Latino Men: The Mediating Effects of Mental Health
Authors: Maldonado Ana Isabel , Cunradi Carol B. , Nápoles Anna María
Published: November 2020

Purpose: Intimate partner violence (IPV) is a serious public health problem that disproportionately affects racial/ethnic minorities in the U.S. This study examines risk factors for IPV perpetration that are salient for racial/ethnic minorities; specifically, we test if racial/ethnic discrimination among Latino men is associated with IPV perpetration, if poor mental health (MH) mediates this link, and whether relationships differ by immigrant status. Methods: Using National Epidemiologic Survey on Alcohol and Related Conditions (NESARC-II) Wave 2 (2004–2005) data, multigroup structural equation modeling compared immigrant (N = 1187) and U.S.-born (N = 1077) Latinos on a mediation model whereby discrimination increases IPV risk via poor MH (anxiety, depression, post-traumatic stress (PTSS); alcohol dependence (AD) and drug dependence (DD)). Results: For U.S.-born Latinos, discrimination increased anxiety (β = 0.24, p < 0.001), depression (β = 0.16, p < 0.001), PTSS (β = 0.09, p < 0.001), AD (β = 0.11, p < 0.001) and DD (β = 0.16, p < 0.001); anxiety (β = 0.16, p < 0.001), AD (β = 0.19, p < 0.001) and DD (β = 0.09, p < 0.01) increased IPV risk. Among Latino immigrants, discrimination increased anxiety (β = 0.07, p < 0.001), depression (β = 0.16, p < 0.001), PTSS (β = 0.08, p < 0.001) and DD (β = 0.03, p < 0.001); PTSS (β = 0.16, p < 0.001), AD (β = 0.21, p < 0.001) and DD (β = 0.05, p < 0.01) increased IPV risk. Conclusions: Among Latino men, discrimination is associated with poorer MH and contributes to IPV perpetration; MH risk factors vary by immigrant status.
A Parental Smoking Cessation Intervention in the Pediatric Emergency Setting: A Randomized Trial
Authors: Mahabee-Gittens E. Melinda , Ammerman Robert T. , Khoury Jane C. , Tabangin Meredith E. , Ding Lili , Merianos Ashley L. , Stone Lara , Gordon Judith S.
Published: November 2020

We examined the efficacy of a pediatric emergency visit-based screening, brief intervention, and referral to treatment (SBIRT) condition compared to a control condition (Healthy Habits Control, HHC) to help parental smokers quit smoking. We enrolled 750 parental smokers who presented to the pediatric emergency setting with their child into a two-group randomized controlled clinical trial. SBIRT participants received brief cessation coaching, quitting resources, and up to 12-weeks of nicotine replacement therapy (NRT). HHC participants received healthy lifestyle coaching and resources. The primary outcome was point-prevalence tobacco abstinence at six weeks (T1) and six months (T2). The mean (SD) age of parents was 31.8 (7.7) years, and 86.8% were female, 52.7% were Black, and 64.6% had an income of ≤$15,000. Overall abstinence rates were not statistically significant with 4.2% in both groups at T1 and 12.9% and 8.3% in the SBIRT and HHC groups, respectively, at T2. There were statistically significant differences in SBIRT versus HHC participants on the median (IQR) reduction of daily cigarettes smoked at T1 from baseline (−2 [−5, 0] versus 0 [−4, 0], p = 0.0008),at T2 from baseline (−4 [−9, −1] vs. −2 [−5, 0], p = 0.0006), and on the mean (SD) number of quit attempts at T2 from baseline (1.25 (6.5) vs. 0.02 (4.71), p = 0.02). Self-reported quitting rates were higher in SBIRT parents who received NRT (83.3% vs. 50.9%, p = 0.04). The novel use of the pediatric emergency visit to conduct cessation interventions helped parents quit smoking. The near equivalent abstinence rates in both the SBIRT and HHC groups may be due to underlying parental concern about their child’s health. Cessation interventions in this setting may result in adult and pediatric public health benefits.
Cyclin Y Is Expressed in Platelets and Modulates Integrin Outside-in Signaling
Authors: Kyselova Anastasia , Siragusa Mauro , Anthes Julian , Solari Fiorella Andrea , Loroch Stefan , Zahedi René P. , Walter Ulrich , Fleming Ingrid , Randriamboavonjy Voahanginirina
Published: November 2020

Diabetes is associated with platelet hyper-reactivity and enhanced risk of thrombosis development. Here we compared protein expression in platelets from healthy donors and diabetic patients to identify differentially expressed proteins and their possible function in platelet activation. Mass spectrometry analyses identified cyclin Y (CCNY) in platelets and its reduced expression in platelets from diabetic patients, a phenomenon that could be attributed to the increased activity of calpains. To determine the role of CCNY in platelets, mice globally lacking the protein were studied. CCNY-/- mice demonstrated lower numbers of circulating platelets but platelet responsiveness to thrombin and a thromboxane A2 analogue were comparable with that of wild-type mice, as was agonist-induced α and dense granule secretion. CCNY-deficient platelets demonstrated enhanced adhesion to fibronectin and collagen as well as an attenuated spreading and clot retraction, indicating an alteration in “outside in” integrin signalling. This phenotype was accompanied by a significant reduction in the agonist-induced tyrosine phosphorylation of β3 integrin. Taken together we have shown that CCNY is present in anucleated platelets where it is involved in the regulation of integrin-mediated outside in signalling associated with thrombin stimulation.
Neovascular Age-Related Macular Degeneration: Therapeutic Management and New-Upcoming Approaches
Authors: Ricci Federico , Bandello Francesco , Navarra Pierluigi , Staurenghi Giovanni , Stumpp Michael , Zarbin Marco
Published: November 2020

Age-related macular degeneration (AMD) constitutes a prevalent, chronic, and progressive retinal degenerative disease of the macula that affects elderly people and cause central vision impairment. Despite therapeutic advances in the management of neovascular AMD, none of the currently used treatments cures the disease or reverses its course. Medical treatment of neovascular AMD experienced a significant advance due to the introduction of vascular endothelial growth factor inhibitors (anti-VEGF), which dramatically changed the prognosis of the disease. However, although anti-VEGF therapy has become the standard treatment for neovascular AMD, many patients do not respond adequately to this therapy or experience a slow loss of efficacy of anti-VEGF agents after repeated administration. Additionally, current treatment with intravitreal anti-VEGF agents is associated with a significant treatment burden for patients, caregivers, and physicians. New approaches have been proposed for treating neovascular AMD. Among them, designed ankyrin repeat proteins (DARPins) seem to be as effective as monthly ranibizumab, but with greater durability, which may enhance patient compliance with needed injections.
Role of Phosphodiesterase in the Biology and Pathology of Diabetes
Authors: Kilanowska Agnieszka , Ziółkowska Agnieszka
Published: November 2020

Glucose metabolism is the initiator of a large number of molecular secretory processes in β cells. Cyclic nucleotides as a second messenger are the main physiological regulators of these processes and are functionally divided into compartments in pancreatic cells. Their intracellular concentration is limited by hydrolysis led by one or more phosphodiesterase (PDE) isoenzymes. Literature data confirmed multiple expressions of PDEs subtypes, but the specific roles of each in pancreatic β-cell function, particularly in humans, are still unclear. Isoforms present in the pancreas are also found in various tissues of the body. Normoglycemia and its strict control are supported by the appropriate release of insulin from the pancreas and the action of insulin in peripheral tissues, including processes related to homeostasis, the regulation of which is based on the PDE- cyclic AMP (cAMP) signaling pathway. The challenge in developing a therapeutic solution based on GSIS (glucose-stimulated insulin secretion) enhancers targeted at PDEs is the selective inhibition of their activity only within β cells. Undeniably, PDEs inhibitors have therapeutic potential, but some of them are burdened with certain adverse effects. Therefore, the chance to use knowledge in this field for diabetes treatment has been postulated for a long time.
Brain Distribution of Dual ABCB1/ABCG2 Substrates Is Unaltered in a Beta-Amyloidosis Mouse Model
Authors: Wanek Thomas , Zoufal Viktoria , Brackhan Mirjam , Krohn Markus , Mairinger Severin , Filip Thomas , Sauberer Michael , Stanek Johann , Pekar Thomas , Pahnke Jens , Langer Oliver
Published: November 2020

Background: ABCB1 (P-glycoprotein) and ABCG2 (breast cancer resistance protein) are co-localized at the blood-brain barrier (BBB), where they restrict the brain distribution of many different drugs. Moreover, ABCB1 and possibly ABCG2 play a role in Alzheimer’s disease (AD) by mediating the brain clearance of beta-amyloid (Aβ) across the BBB. This study aimed to compare the abundance and activity of ABCG2 in a commonly used β-amyloidosis mouse model (APP/PS1-21) with age-matched wild-type mice. Methods: The abundance of ABCG2 was assessed by semi-quantitative immunohistochemical analysis of brain slices of APP/PS1-21 and wild-type mice aged 6 months. Moreover, the brain distribution of two dual ABCB1/ABCG2 substrate radiotracers ([11C]tariquidar and [11C]erlotinib) was assessed in APP/PS1-21 and wild-type mice with positron emission tomography (PET). [11C]Tariquidar PET scans were performed without and with partial inhibition of ABCG2 with Ko143, while [11C]erlotinib PET scans were only performed under baseline conditions. Results: Immunohistochemical analysis revealed a significant reduction (by 29–37%) in the number of ABCG2-stained microvessels in the brains of APP/PS1-21 mice. Partial ABCG2 inhibition significantly increased the brain distribution of [11C]tariquidar in APP/PS1-21 and wild-type mice, but the brain distribution of [11C]tariquidar did not differ under both conditions between the two mouse strains. Similar results were obtained with [11C]erlotinib. Conclusions: Despite a reduction in the abundance of cerebral ABCG2 and ABCB1 in APP/PS1-21 mice, the brain distribution of two dual ABCB1/ABCG2 substrates was unaltered. Our results suggest that the brain distribution of clinically used ABCB1/ABCG2 substrate drugs may not differ between AD patients and healthy people.
Compression Bioreactor-Based Mechanical Loading Induces Mobilization of Human Bone Marrow-Derived Mesenchymal Stromal Cells into Collagen Scaffolds In Vitro
Authors: Gamez Carolina , Schneider-Wald Barbara , Bieback Karen , Schuette Andy , Büttner Sylvia , Hafner Mathias , Gretz Norbert , Schwarz Markus L.
Published: November 2020

Articular cartilage (AC) is an avascular tissue composed of scattered chondrocytes embedded in a dense extracellular matrix, in which nourishment takes place via the synovial fluid at the surface. AC has a limited intrinsic healing capacity, and thus mainly surgical techniques have been used to relieve pain and improve function. Approaches to promote regeneration remain challenging. The microfracture (MF) approach targets the bone marrow (BM) as a source of factors and progenitor cells to heal chondral defects in situ by opening small holes in the subchondral bone. However, the original function of AC is not obtained yet. We hypothesize that mechanical stimulation can mobilize mesenchymal stromal cells (MSCs) from BM reservoirs upon MF of the subchondral bone. Thus, the aim of this study was to compare the counts of mobilized human BM-MSCs (hBM-MSCs) in alginate-laminin (alginate-Ln) or collagen-I (col-I) scaffolds upon intermittent mechanical loading. The mechanical set up within an established bioreactor consisted of 10% strain, 0.3 Hz, breaks of 10 s every 180 cycles for 24 h. Contrary to previous findings using porcine MSCs, no significant cell count was found for hBM-MSCs into alginate-Ln scaffolds upon mechanical stimulation (8 ± 5 viable cells/mm3 for loaded and 4 ± 2 viable cells/mm3 for unloaded alginate-Ln scaffolds). However, intermittent mechanical stimulation induced the mobilization of hBM-MSCs into col-I scaffolds 10-fold compared to the unloaded col-I controls (245 ± 42 viable cells/mm3 vs. 22 ± 6 viable cells/mm3, respectively; p-value < 0.0001). Cells that mobilized into the scaffolds by mechanical loading did not show morphological changes. This study confirmed that hBM-MSCs can be mobilized in vitro from a reservoir toward col-I but not alginate-Ln scaffolds upon intermittent mechanical loading, against gravity.
Current Evidence and Future Perspectives on Pharmacological Treatment of Calcific Aortic Valve Stenosis
Authors: Donato Maristella , Ferri Nicola , Lupo Maria Giovanna , Faggin Elisabetta , Rattazzi Marcello
Published: November 2020

Calcific aortic valve stenosis (CAVS), the most common heart valve disease, is characterized by the slow progressive fibro-calcific remodeling of the valve leaflets, leading to progressive obstruction to the blood flow. CAVS is an increasing health care burden and the development of an effective medical treatment is a major medical need. To date, no effective pharmacological therapies have proven to halt or delay its progression to the severe symptomatic stage and aortic valve replacement represents the only available option to improve clinical outcomes and to increase survival. In the present report, the current knowledge and latest advances in the medical management of patients with CAVS are summarized, placing emphasis on lipid-lowering agents, vasoactive drugs, and anti-calcific treatments. In addition, novel potential therapeutic targets recently identified and currently under investigation are reported.
Microstructure and Properties of Tungsten Heavy Alloy Connections Formed during Sintering with the Participation of the Liquid Phase
Authors: Skoczylas Paweł , Gulbinowicz Zbigniew , Goroch Olgierd
Published: November 2020

Tungsten heavy alloys (THA) are used in the defense industry for subcaliber bullet cores due to their high density and strength. Typically methods of joining tungsten rod elements include: soldering, friction welding or threaded sleeve splicing. The properties of the joints were tested for three types of material containing 90.8, 96.2 and 98.2 wt.%. tungsten, density from 17.3 to 18.4 g/cm3 and strength range 400–1000 MPa. Combination in the liquid phase at the sintering temperature was carried out in a vacuum furnace at a temperature of 1520 °C in a hydrogen atmosphere, and tests used pairs of both identical and dissimilar materials. After that, some of the bars were subjected to additional heat treatment at 1100 °C for 3 h. The tests of the mechanical properties in the static tensile test and the measurement of impact strength showed that the obtained strength of the joints was comparable to that of the parent material. The microstructure analysis showed that the resulting joint area, while maintaining the appropriate roughness of the joined end faces of the bars, is homogeneous without areas of the solidified matrix of the joint line. Research showed that it is possible to bond under sintering conditions with the participation of a solid liquid phase of homonymous and dissimilar THA materials. The strength of joints in dissimilar materials was comparable to a tungsten heavy alloy material with lower strength in the bonded pair while homonymous materials were comparable to the original material. The test results provided a good basis for further research in which the obtained pairs of joints will be subjected to plastic working processes.
Discrete Predictive Models for Stability Analysis of Power Supply Systems
Authors: Bakhtadze Natalia , Yadikin Igor
Published: November 2020

The paper offers an approach to the investigation of the dynamics of nonlinear non-stationary processes with the focus on the risk of dynamic system stability loss. The risk is assessed on the basis of the accumulated knowledge about power supply system operation. New methods for power supply modes analysis are developed and applied as follows: linear discrete point knowledge-based models are developed for nonlinear non-stationary objects; wavelet analysis is used for non-stationary processes; stability loss risks are analyzed through the investigation of spectral decompositions of Gramians of these linear predictive models. Case studies are included.
Distributed Fusion Estimation with Sensor Gain Degradation and Markovian Delays
Authors: García-Ligero María Jesús , Hermoso-Carazo Aurora , Linares-Pérez Josefa
Published: November 2020

This paper investigates the distributed fusion estimation of a signal for a class of multi-sensor systems with random uncertainties both in the sensor outputs and during the transmission connections. The measured outputs are assumed to be affected by multiplicative noises, which degrade the signal, and delays may occur during transmission. These uncertainties are commonly described by means of independent Bernoulli random variables. In the present paper, the model is generalised in two directions: (i) at each sensor, the degradation in the measurements is modelled by sequences of random variables with arbitrary distribution over the interval [0, 1]; (ii) transmission delays are described using three-state homogeneous Markov chains (Markovian delays), thus modelling dependence at different sampling times. Assuming that the measurement noises are correlated and cross-correlated at both simultaneous and consecutive sampling times, and that the evolution of the signal process is unknown, we address the problem of signal estimation in terms of covariances, using the following distributed fusion method. First, the local filtering and fixed-point smoothing algorithms are obtained by an innovation approach. Then, the corresponding distributed fusion estimators are obtained as a matrix-weighted linear combination of the local ones, using the mean squared error as the criterion of optimality. Finally, the efficiency of the algorithms obtained, measured by estimation error covariance matrices, is shown by a numerical simulation example.
Who Knocks on the Door of Portfolio Performance Heaven: Sinner or Saint Investors?
Authors: Miralles-Quirós José Luis , Miralles-Quirós María Mar
Published: November 2020

To sin, or not to sin: that has been the question for many people for a long time, and nowadays that question has moved to the financial markets. The existence of studies that show that investing in vice sectors such as the alcohol, tobacco, and gambling industries, collectively known as the “triumvirate of Sin”, is profitable has created some uncertainty for investors who wonder whether or not to be socially responsible. We show that by implementing an investment strategy based on the Fama–French five-factor model, “saint” investors obtain better portfolio performance, even when transaction costs are taken into consideration, and therefore they are the ones chosen to knock on the door of portfolio performance heaven.
Improving Food Supply Chain Management by a Sustainable Approach to Supplier Evaluation
Authors: Segura Marina , Maroto Concepción , Segura Baldomero , Casas-Rosal José Carlos
Published: November 2020

Increasing food supply chain sustainability means having to deal with many conflicting aspects and involves producers, several departments in distribution companies, and consumers. The objectives of this research are to develop models to solve real-world supplier evaluation problems and validate them with real data on fresh fruits in a supermarket chain. Literature review and results from a survey with managers from purchasing, logistics, and quality departments of a food distribution company are used to establish criteria, to first model the assessment of products and, second, to model supplier evaluation. A multicriteria hybrid approach is proposed, using multi-attribute utility theory (MAUT) to assess the quality of products and Preference Ranking Organisation Method for Enrichment Evaluation (PROMETHEE) to complete their evaluation with strategic criteria to be included in the second phase. The results allow companies to rank suppliers by product and classify them according to the main criteria categories, such as product strategy, food safety, economic, logistic, commercial, green image and corporate social responsibility. A sorting approach is also applied to obtain ordered groups of suppliers. Finally, the models proposed can form the core of a decision support system in order to create and monitor the supplier base in food distribution companies, as well as to inform sustainable decision making.
Impact of the Post-Transplant Period and Lifestyle Diseases on Human Gut Microbiota in Kidney Graft Recipients
Authors: Souai Nessrine , Zidi Oumaima , Mosbah Amor , Kosai Imen , Manaa Jameleddine El , Mokhtar Naima Bel , Asimakis Elias , Stathopoulou Panagiota , Cherif Ameur , Tsiamis George , Kouidhi Soumaya
Published: November 2020

Gaining long-term graft function and patient life quality remain critical challenges following kidney transplantation. Advances in immunology, gnotobiotics, and culture-independent molecular techniques have provided growing insights into the complex relationship of the microbiome and the host. However, little is known about the over time-shift of the gut microbiota in the context of kidney transplantation and its impact on both graft and health stability. Here we aimed to characterize the structure of gut microbiota within stable kidney graft recipients. We enrolled forty kidney transplant patients after at least three months of transplantation and compared them to eighteen healthy controls. The overall microbial community structure of the kidney transplanted group was clearly different from control subjects. We found lower relative abundances of Actinobacteria, Bacteroidetes, and Verrucomicrobia within the patient group and a higher abundance of Proteobacteria compared to the control group. Both richness and Shannon diversity indexes were significantly lower in the kidney graft recipients than in healthy controls. Post-graft period was positively correlated with the relative abundance of the Proteobacteria phylum, especially Escherichia.Shigella genus. Interestingly, only Parabacteroides was found to significantly differentiate patients that were not suffering from lifestyle diseases and those who suffer from post-graft complications. Furthermore, network analysis showed that the occurrence of lifestyle diseases was significantly linked with a higher number of negative interactions of Sutterella and Succinivibrio genera within patients. This study characterizes gut microbiome fluctuation in stable kidney transplant patients after a long post-allograft period. Analysis of fecal microbiota could be useful for nephrologists as a new clinical tool that can improve kidney allograft monitoring and outcomes.
Effect of Schwertmannite Surface Modification by Surfactants on Adhesion of Acidophilic Bacteria
Authors: Pawlowska Agnieszka , Sadowski Zygmunt
Published: November 2020

Bacterial cell adhesion onto mineral surfaces is important in a broad spectrum of processes, including bioweathering, bioleaching, and bacterial cell transport in the soil. Despite many research efforts, a detailed explanation is still lacking. This work investigates the role of surface-active compounds, cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), and pure rhamnolipid (RH), in the process of bacteria attachment on the schwertmannite surface. The surface energy was calculated based on the wettability of the tested systems, and for bacteria it was 54.8 mJ/m2, schwertmannite-SDS 54.4 mJ/m2, schwertmannite-CTAB 55.4 mJ/m2, and schwertmannite-RH 39.7 mJ/m2. The total energy of adhesion estimated based on thermodynamic data was found to be negative, suggesting favorable conditions for adhesion for all examined suspensions. However, including electrostatic interactions allowed for a more precise description of bacterial adhesion under the tested conditions. The theoretical analysis using the extended Derjaguin-Landau-Verwey-Overbeek (DLVO) approach showed a negative value of total adsorption energy only in bacteria-mineral suspensions, where SDS and rhamnolipid were added. The calculated data were in good agreement with experimental results indicating the significance of electrostatic forces in adsorption.
Gut Microbiome and Its Interaction with Immune System in Spondyloarthritis
Authors: So Jacqueline , Tam Lai-Shan
Published: November 2020

Emerging evidence suggests there is a gut-joint axis in spondyloarthritis (SpA). In a study, subclinical gut inflammation occurred in nearly 50% of SpA. Chronic gut inflammation also correlated with disease activity in SpA. Trillions of microorganisms reside in the human gut and interact with the human immune system. Dysbiosis affects gut immune homeostasis and triggers different autoimmune diseases including SpA. The absence of arthritis in HLA-B27 germ-free mice and the development of arthritis after the introduction of commensal bacteria to HLA-B27 germ-free mice proved to be the important role of gut bacteria in shaping SpA, other than the genetic factor. The recent advance in gene sequencing technology promotes the identification of microorganisms. In this review, we highlighted current evidence supporting the link between gut and axial SpA (axSpA). We also summarized available findings of gut microbiota and its interaction with the immune system in axSpA. Future research may explore the way to modulate gut microorganisms in axSpA and bring gut microbiome discoveries towards application.
Bacterial Endotoxin Testing—Fast Endotoxin Masking Kinetics in the Presence of Lauryldimethylamine Oxide
Authors: Bech Ørving René , Carpenter Bill , Roth Steffen , Reich Johannes , Kallipolitis Birgitte H. , Sonne-Hansen Jacob
Published: November 2020

For release of parenteral drug products, bacterial endotoxin testing is one of a panel of necessary tests. In order to ensure the validity of such tests, various controls are performed, including demonstration of compendial method suitability or method qualification. In addition to compendial suitability testing, quality control (QC) sample hold-time studies are requested by authorities like the Food and Drug Administration (FDA) as described in “Guidance for Industry: Pyrogen and Endotoxins Testing.” It is requested to be determine whether the ability to detect endotoxins can be affected by storage and handling of the sample to be tested. To accomplish these studies, endotoxin is introduced or spiked into the undiluted product and held for a certain period of time in process-representative containers. This time period reflects procedural maximum QC sample hold time from sampling until analysis. Inadequate detection of endotoxin can be caused by adsorption of endotoxin to container surfaces or molecular masking effects, in which the binding sites on the endotoxin molecules are prevented from triggering the enzymatic cascade necessary in the assay, are obscured. The endotoxin may form macromolecular structures, such as sheets or blebs, or the binding sites may otherwise be rendered unavailable due to the sample matrix composition. In either case, the endotoxin assay may yield falsely low results if and when masking occurs. In this work, the QC sample hold times of different in-process controls within the production process of a biopharmaceutical product were analyzed. One out of eight different samples showed a strong masking of endotoxin. Analysis of the sample composition revealed that either kifunensine, mycophenolic acid (MPA), or lauryl-N, N-dimethylamine oxide (LDAO) was responsible for masking. Further analysis clearly identified LDAO as the root cause for masking. A novel one-step mechanism for LDAO-induced endotoxin masking is proposed. The principle is similar to an already-proposed two-step mechanism for endotoxin masking, but the LDAO case combines these two steps: the disturbance of the salt bridges and hydrophobic interactions with LPS in one molecule. These molecular interactions occur quickly when both endotoxin and LDAO are present in the same matrix. Thus, depending on the masking agents, low endotoxin recovery (LER) can occur regardless of the QC sample hold duration.
Prospective Evaluation of Mango Fruit Intake on Facial Wrinkles and Erythema in Postmenopausal Women: A Randomized Clinical Pilot Study
Authors: Fam Vivien W. , Holt Roberta R. , Keen Carl L. , Sivamani Raja K. , Hackman Robert M.
Published: November 2020

Mangos are rich in β-carotene and other carotenoids, along with several phenolic acids that may provide oxidant defense and photoprotection to the skin. The objectives of this study are to investigate the effects of Ataulfo mango intake on the development of facial wrinkles and erythema. A randomized two-group parallel-arm study was conducted to assess 16 weeks of either 85 g or 250 g of mango intake in healthy postmenopausal women with Fitzpatrick skin type II or III. Facial photographs were captured at weeks 0, 8, and 16, and wrinkles at the lateral canthi and erythema at the cheeks were quantified. Skin carotenoid values were measured with reflection spectroscopy. Deep wrinkle severity decreased significantly in the 85 g group after 8 (p = 0.007) and 16 (p = 0.03) weeks compared to baseline measures. In contrast, those in the 250 g group showed an increase after 16 weeks in average wrinkle severity (p = 0.049), average wrinkle length (p = 0.007), fine wrinkle severity (p = 0.02), and emerging wrinkle severity (p = 0.02). Erythema in the cheeks increased with 85 g of mango intake (p = 0.04). The intake of 85 g of mangos reduced wrinkles in fair-skinned postmenopausal women, while an intake of 250 g showed the opposite effect. Further studies feeding 85 g of mangos are warranted.
Multinutrients for the Treatment of Psychiatric Symptoms in Clinical Samples: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Johnstone Jeanette M. , Hughes Andrew , Goldenberg Joshua Z. , Romijn Amy R. , Rucklidge Julia J.
Published: November 2020

This systematic review and meta-analysis focused on randomized controlled trials (RCT) of multinutrients consisting of at least four vitamins and/or minerals as interventions for participants with psychiatric symptoms. A systematic search identified 16 RCTs that fit the inclusion criteria (n = 1719 participants) in six psychiatric categories: depression, post-disaster stress, antisocial behavior, behavioral deficits in dementia, attention-deficit/hyperactivity disorder, and autism. Grading of Recommendations, Assessment, Development and Evaluations (GRADE) was used to rate the evidence base. Significant clinical benefit was assessed using minimal clinically important differences (MIDs). Due to heterogeneity in participants, multinutrient formulas, outcome measures, and absence of complete data, only the Attention-Deficit/Hyperactivity Disorder (ADHD) category was eligible for meta-analyses. In ADHD populations, statistically and clinically significant improvements were found in global functioning, Mean Difference (MD) −3.3, p = 0.001, MID −3.26; Standardized Mean Difference (SMD) −0.49 p = 0.001 MD −0.5), clinician ratings of global improvement (MD −0.58, p = 0.001, MID −0.5) and ADHD improvement (MD −0.54, p = 0.002, MID −0.5), and clinician (but not observer) measures of ADHD inattentive symptoms (MD −1.53, p = 0.05, MID −0.5). Narrative synthesis also revealed a pattern of benefit for global measures of improvement, for example: in autism, and in participants with behavioral deficits in dementia. Post-natural disaster anxiety and the number of violent incidents in prison populations also improved. Broad-spectrum formulas (vitamins + minerals) demonstrated more robust effects than formulas with fewer ingredients. This review highlights the need for robust methodology-RCTs that report full data, including means and standard deviations for all outcomes-in order to further elucidate the effects of multinutrients for psychiatric symptoms.
Controlled Drug Release from Biodegradable Polymer Matrix Loaded in Microcontainers Using Hot Punching
Authors: Petersen Ritika Singh , Nielsen Line Hagner , Rindzevicius Tomas , Boisen Anja , Keller Stephan Sylvest
Published: November 2020

Microcontainers are reservoir-based advanced drug delivery systems (DDS) that have proven to increase the bioavailibity of the small-molecule drugs, targeting of biomolecules, protection of vaccines and improved treatment of Pseudomonas aeruginosa. However, high-throughput loading of these micron-sized devices with drug has been challenging. Hot punching is a new technique that is a fast, simple and single-step process where the microdevices are themselves used as mold to punch biocompatible and biodegradable drug-polymer films, thereby loading the containers. Here, we investigate the effect of hot punching on the drug distribution as well as drug release from the loaded drug-polymer matrices. Zero-order sustained drug release is observed for the model drug Furosemide embedded in biodegradable polymer, Poly-ε-caprolactone, which is attributed to the unique spatial distribution of Furosemide during the loading process.
Numerical Simulation and Experimental Research on Flow Force and Pressure Stability in a Nozzle-Flapper Servo Valve
Authors: Kang Jian , Yuan Zhaohui , Tariq Sadiq Muhammad
Published: November 2020

In the nozzle flapper servo valve, the transient flow force on the flapper is the fundamental reason that affects the pressure stability. The pressure pulsation in the pilot stage causes forced vibration of the flapper, and its deviation will directly influence the control pressure difference, which will make the pressure appear unstable. In order to grasp the principle and characteristics of transient flow force and its influence on pressure stability, a mathematical model of flapper displacement and control pressure is derived. For collecting the dynamic changes of the transient flow force and recording the motion behavior of the flapper, a three-dimensional model of the pilot-stage is established. Numerical simulations of turbulence phenomenon analysis are conducted with a variation of flapper displacement ranging from 5 μm to 20 μm. It can be concluded that the change trend of the flapper displacement is similar to the steady-state flow force and the transient flow force pulsation amplitude. Under the same structural parameters, the pulsating frequency of the flow force remains basically constant. The fluctuation of the flow force of the pilot stage will cause the pressure of the servo valve control cavity to vibrate to a certain extent, which is a factor that cannot be ignored that affects the output stability of the servo valve.
Digital Competence in Spanish University Education and Its Use by Students
Authors: Araújo-Vila Noelia , Cardoso Lucília , Toubes Diego R. , Fraiz-Brea Jose Antonio
Published: November 2020

Technologies have massively burst into all fields, including Higher Education. The current students have grown up surrounded by technologies, which is reflected in their behavior. For this reason, universities have adapted by integrating digital competence into their training offer, improving learning processes and adjusting to the university profile. The objective of this work is to ascertain how digital skills are used by Spanish higher education (bachelor’s and master’s degree) students, thus verifying whether so-called digital competence is being actively used in higher education. A survey was applied to 324 individuals, highlighting among its results that the university panorama is in a situation where digital tools are very useful for its improvement. These data were collected before the global pandemic, after which the use of online tools intensified. However, the students are still not aware of all of them, or they do not use them.
Energy Harvested and Cooperative Enabled Efficient Routing Protocol (EHCRP) for IoT-WBAN
Authors: Khan Muhammad Dawood , Ullah Zahid , Ahmad Arshad , Hayat Bashir , Almogren Ahmad , Kim Kyong Hoon , Ilyas Muhammad , Ali Muhammad
Published: November 2020

The health industry is one of the most auspicious domains for the application of Internet of Things (IoT) based technologies. Lots of studies have been carried out in the health industry field to minimize the use of resources and increase the efficiency. The use of IoT combined with other technologies has brought quality advancement in the health sector at minimum expense. One such technology is the use of wireless body area networks (WBANs), which will help patients incredibly in the future and will make them more productive because there will be no need for staying at home or a hospital for a long time. WBANs and IoT have an integrated future as WBANs, like any IoT application, are a collection of heterogeneous sensor-based devices. For the better amalgamation of the IoT and WBANs, several hindrances blocking their integration need to be addressed. One such problem is the efficient routing of data in limited resource sensor nodes (SNs) in WBANs. To solve this and other problems, such as transmission of duplicate sensed data, limited network lifetime, etc., energy harvested and cooperative-enabled efficient routing protocol (EHCRP) for IoT-WBANs is proposed. The proposed protocol considers multiple parameters of WBANs for efficient routing such as residual energy of SNs, number of hops towards the sink, node congestion levels, signal-to-noise ratio (SNR) and available network bandwidth. A path cost estimation function is calculated to select forwarder node using these parameters. Due to the efficient use of the path-cost estimation process, the proposed mechanism achieves efficient and effective multi-hop routing of data and improves the reliability and efficiency of data transmission over the network. After extensive simulations, the achieved results of the proposed protocol are compared with state-of-the-art techniques, i.e., E-HARP, EB-MADM, PCRP and EERP. The results show significant improvement in network lifetime, network throughout, and end-to-end delay.
Tracking and Characterization of Spinal Cord-Injured Patients by Means of RGB-D Sensors
Authors: Colombo Zefinetti Filippo , Vitali Andrea , Regazzoni Daniele , Rizzi Caterina , Molinero Guido
Published: November 2020

In physical rehabilitation, motion capture solutions are well-known but not as widespread as they could be. The main limit to their diffusion is not related to cost or usability but to the fact that the data generated when tracking a person must be elaborated according to the specific context and aim. This paper proposes a solution including customized motion capture and data elaboration with the aim of supporting medical personnel in the assessment of spinal cord-injured (SCI) patients using a wheelchair. The configuration of the full-body motion capturing system is based on an asymmetric 3 Microsoft Kinect v2 sensor layout that provides a path of up to 6 m, which is required to properly track the wheelchair. Data elaboration is focused on the automatic recognition of the pushing cycles and on plotting any kinematic parameter that may be interesting in the assessment. Five movements have been considered to evaluate the wheelchair propulsion: the humeral elevation, the horizontal abduction of the humerus, the humeral rotation, the elbow flexion and the trunk extension along the sagittal plane. More than 60 volunteers with a spinal cord injury were enrolled for testing the solution. To evaluate the reliability of the data computed with SCI APPlication (APP) for the pushing cycle analysis, the patients were subdivided in four groups according to the level of the spinal cord injury (i.e., high paraplegia, low paraplegia, C7 tetraplegia and C6 tetraplegia). For each group, the average value and the standard deviation were computed and a comparison with similar acquisitions performed with a high-end solution is shown. The measurements computed by the SCI-APP show a good reliability for analyzing the movements of SCI patients’ propulsion wheelchair.
Correction: Liu, W. et al. Error Overboundings of KF-Based IMU/GNSS Integrated System Against IMU Faults. Sensors 2019, 19, 4912
Authors: Liu Wei , Song Dan , Wang Zhipeng , Fang Kun
Published: November 2020

To clearly highlight the differences between the error overboundings of error-state EKFs and full-state EKFs [...]
Estimation of Three-Dimensional Lower Limb Kinetics Data during Walking Using Machine Learning from a Single IMU Attached to the Sacrum
Authors: Lee Myunghyun , Park Sukyung
Published: November 2020

Kinetics data such as ground reaction forces (GRFs) are commonly used as indicators for rehabilitation and sports performance; however, they are difficult to measure with convenient wearable devices. Therefore, researchers have attempted to estimate accurately unmeasured kinetics data with artificial neural networks (ANNs). Because the inputs to an ANN affect its performance, they must be carefully selected. The GRF and center of pressure (CoP) have a mechanical relationship with the center of mass (CoM) in the three dimensions (3D). This biomechanical characteristic can be used to establish an appropriate input and structure of an ANN. In this study, an ANN for estimating gait kinetics with a single inertial measurement unit (IMU) was designed; the kinematics of the IMU placed on the sacrum as a proxy for the CoM kinematics were applied based on the 3D spring mechanics. The walking data from 17 participants walking at various speeds were used to train and validate the ANN. The estimated 3D GRF, CoP trajectory, and joint torques of the lower limbs were reasonably accurate, with normalized root-mean-square errors (NRMSEs) of 6.7% to 15.6%, 8.2% to 20.0%, and 11.4% to 24.1%, respectively. This result implies that the biomechanical characteristics can be used to estimate the complete three-dimensional gait data with an ANN model and a single IMU.
An Ultra-Wide Load Range Voltage Converter Using Proactive Phase Frequency Modulation for IoT Sensors
Authors: Arslan Saad , Shah Syed Asmat Ali , Kim HyungWon
Published: November 2020

Modern sensor nodes have multiple operating states, which causes a conventional voltage converter to perform poorly over a wide load range of the operating states. This paper proposes a voltage converter whose switching frequency and output voltage are proactively adjusted to maintain high conversion efficiency. This allows the converter to exploit a wider frequency range to cover a wide load range. In addition, the proposed converter uses multiple smaller capacitor banks and employs multiphase operation to provide low output ripple voltage. A distributed topology for non-overlapping signal generation is proposed and used in the converter to minimize the number of wires running from connecting the controller to the converter. The proposed voltage converter has been implemented in a chip using a 0.13 um CMOS process. The measurement results demonstrate the ability to support a wide load range of 10 µA to 10 mA, for switching frequencies ranging from 100 kHz to 200 MHz, while providing an efficiency of above 80%.