bluuebunny/crossref_metadata_2025 huggingface dataset:
Sometimes here, sometimes there—Differential effects of social challenge and hindrance stressors depending on the work location
Authors: Rigotti Thomas , Schilbach Miriam , Kern Marcel
Published: March 2024

Introduction

As a result of the COVID-19 pandemic, the number of employees working from home has more than tripled in Germany from 2019 to 2022. While earlier research on remote work primarily focused on discerning variations among employee groups, this study delves into the realm of intra-individual effects. Specifically, we investigate how social stressors relate to exhaustion and positive affect at the end of the day within the context of different work settings.

Methods

This research draws insights from a sample comprising 99 employed individuals who diligently responded to surveys over a span of up to 10 working days (with an average of 6 days) after work and prior to bedtime.

Results

Although the absolute level of encountered social stressors exhibited no noteworthy difference between working at home or the premise of the employer, the relationship to exhaustion was moderated by the work location. Remarkably, the positive link between challenge-oriented social stressors and exhaustion manifested solely on days spent working from home. In contrast, social hindrance stressors exhibited a positive association with exhaustion exclusively on days when employees were operating within the organizational premise.

Discussion

These findings offer preliminary indications that the significance of specific stressors might indeed fluctuate based on the physical location of work. In essence, this study sheds light on the nuanced interplay between stressors and employee wellbeing, thereby adding depth to our comprehension of the remote work landscape.
Comparative and phylogenetic analyses based on the complete chloroplast genome of Cornus subg. Syncarpea (Cornaceae) species
Authors: Guan Bicai , Wen Jianteng , Guo Hanjing , Liu Yizhen
Published: March 2024

This study presents a comprehensive analysis of the chloroplast (cp) genomes of Cornus species, including comparative and phylogenetic evaluations, as well as examinations of their genomic structure and composition. The cp genomes exhibit a typical circular quadripartite structure and demonstrate highly similar gene order and genomic structure. The complete cp genome size of the 10 taxa in this study is 156,965 bp to 157,383 bp, where the length of the large single-copy (LSC) region is 86,296 bp to 86,691 bp, small single-copy (SSC) region is 18,386 bp to 18,454 bp, and inverted repeat (IR) region is 23,143 bp to 26,112 bp. A total of 131 genes were found, including 86 protein-coding genes (PCGs), eight rRNA genes, and 37 tRNA genes. The mean GC content of the 10 taxa is 38.145%, where the LSC region is 36.396%, the SSC region is 32.372%, and the IR region is 43.076%. Despite the relatively conserved nature of the cp genome within the species of Cornus, 25–31 simple sequence repeats (SSRs) were identified in the 10 taxa in our study. The SSRs were found to be distributed in the LSC, SSC, and IR regions in Cornus hongkongensis subsp. hongkongensis, C. hongkongensis subsp. elegans, C. hongkongensis subsp. gigantea, and C. hongkongensis subsp. tonkinensis, while the SSR was not found in the IR region of the other six taxa. Thus, whole cp genomics is a valuable tool for species identification, taxonomic clarification, and genomic evolutionary analysis. Furthermore, our findings reveal that C. hongkongensis and C. hongkongensis subsp. gigantea, along with Cornus kousa and Cornus elliptica, form sister groups. Notably, C. hongkongensis subsp. ferruginea and C. hongkongensis subsp. melanotricha did not exhibit affinity with C. hongkongensis subsp. hongkongensis. Our study furnishes essential data for further research on their classification and provides novel insights into the relationship within Cornus subg. Syncarpea.
Improvement of Panax notoginseng saponin accumulation triggered by methyl jasmonate under arbuscular mycorrhizal fungi
Authors: Dai Hong-Yang , Zhang Xing-Kai , Bi Yue , Chen Di , Long Xian-Nv , Wu Yue , Cao Guan-Hua , He Sen
Published: March 2024

Panax notoginseng is a highly valued perennial medicinal herb plant in Yunnan Province, China, and the taproots are the main medicinal parts that are rich in active substances of P. notoginseng saponins. The main purpose of this study is to uncover the physiological and molecular mechanism of Panax notoginseng saponin accumulation triggered by methyl jasmonate (MeJA) under arbuscular mycorrhizal fungi (AMF) by determining physiological indices, high-throughput sequencing and correlation analysis. Physiological results showed that the biomass and saponin contents of P. notoginseng, the concentrations of jasmonic acids (JAs) and the key enzyme activities involved in notoginsenoside biosynthesis significantly increased under AMF or MeJA, but the interactive treatment of AMF and MeJA weakened the effect of AMF, suggesting that a high concentration of endogenous JA have inhibitory effect. Transcriptome sequencing results indicated that differential expressed genes (DEGs) involved in notoginsenoside and JA biosynthesis were significantly enriched in response to AMF induction, e.g., upregulated genes of diphosphocytidyl-2-C-methyl-d-erythritol kinases (ISPEs), cytochrome P450 monooxygenases (CYP450s)_and glycosyltransferases (GTs), while treatments AMF-MeJA and salicylhydroxamic acid (SHAM) decreased the abundance of these DEGs. Interestingly, a high correlation presented between any two of saponin contents, key enzyme activities and expression levels of DEGs. Taken together, the inoculation of AMF can improve the growth and saponin accumulation of P. notoginseng by strengthening the activities of key enzymes and the expression levels of encoding genes, in which the JA regulatory pathway is a key link. This study provides references for implementing ecological planting of P. notoginseng, improving saponin accumulation and illustrating the biosynthesis mechanism.
Enhancing the annual yield via nitrogen fertilizer application optimization in the direct seeding ratoon rice system
Authors: Li Yang , Zhang Zuolin , Wang Benfu , Zhang Zhisheng , Lin Yiyue , Cheng Jianping
Published: March 2024

Direct seeding ratoon rice (DSRR) system is a planting method that can significantly increase grain yield, improving light and temperature utilization efficiency and reducing labor input. However, the current nitrogen fertilizer management method which does not aim at the seedling emergence and development characteristics of DSRR just is only based on the traditional method of transplanting ratoon rice, and which is not conducive to the population development and yield improvement. To determine the suitable nitrogen fertilizer application optimization, we set four nitrogen fertilizer application treatments (N0, no nitrogen fertilizer; N1, traditional nitrogen fertilizer; N2, transferring 20% of total nitrogen from basal fertilizer to tillering stage; N3, reducing total nitrogen by 10% from N2 tillering fertilizer) on a hybrid rice “Fengliangyouxiang1 (FLYX1)” and an inbred rice “Huanghuazhan (HHZ)” under DSRR. The effects of treatments on dry matter accumulation, root growth and vigor, leaf area index, leaf senescence rate and yield were investigated. Our results demonstrated that the yield of main crop in N2 treatment was the highest, which was 63.3%, 6.6% and 8.8% higher than that of N0, N1 and N3 treatment, respectively, mainly due to the difference of effective panicle and spikelets number per m2. The average of two years and varieties, the annual yield of N2 was significant higher than that of N1 and N3 by 4.94% and 8.55%, respectively. However, there was no significant difference between the annual yields of N1 and N3. N2 treatment had significant effects on the accumulation of aboveground dry matter mass which was no significant difference in 20 days after sowing(DAS), but significant difference in 50 DAS. Meanwhile, the root activity and the leaf senescence rate of N2 treatment was significant lower than that of other treatments. In summary, “20% of total nitrogen was transferred from basal fertilizer to tillering stage” can improve the annual yield and main crop development of DSRR system. Further reducing the use of nitrogen fertilizer may significantly improve the production efficiency of nitrogen fertilizer and improve the planting income in DSRR system.
COVID-19 vaccines breakthrough infection and adverse reactions in medical students: a nationwide survey in Iran
Authors: Allahgholipour Amirreza , Safavi-Naini Seyed Amir Ahmad , Shekarriz Foomany Zahra , Eskandari Abdolvahab , Nazari Rostami Hossein , Honarvar Mohamad Javad , Mohammadi Mohammad , Khalili Parnian , Ilaghi Mehran , Afshar Hossein , Amini Baghbadorani Ali , Moghimi Hamid Reza , Chamani Goorabi Alireza , Mehrparvar Amirreza , Safari Mehdi , Nakhli Ashraf Sadat , Mahmoudabadi Mohammad , Seifadini Adib , Sheikhansari Sobhan , Khojastehfar Sadaf , Mahdavi Parisa , Mohammadi Maede , Ashrafi Barzideh Siyamak , Akbarzadeh Nadia , Delavarpour Moghadam Seyed Hosein , Tavakoli Pirzaman Ali , Barary Mohammad , Emamhadi MohammadAli
Published: March 2024

Introduction

There are different types of COVID-19 vaccines approved worldwide. Since no national studies focus on vaccine-related adverse reactions and breakthrough cases, this study aimed to investigate the rate of adverse events and COVID-19 infection in medical students in Iran.

Methods

This retrospective cohort study included Iranian medical students who received two doses of COVID-19 vaccines. The medical team gathered the demographic characteristics, comorbidities, type of vaccine, adverse events following vaccination, and history of COVID-19 infection data through a phone interview. The frequency of adverse events and breakthrough infection was stratified by vaccine type (ChAdOx1-S, Gam-COVID-Vac, and BIBP-CorV).

Results

A total of 3,591 medical students enrolled in this study, of which 57.02% were females, with a mean age of 23.31 + 4.87. A PCR-confirmed and suspicious-for-COVID-19 breakthrough infection rate of 4.51 and 7.02% was detected, respectively. There was no significant relation between breakthrough infection and gender, BMI, blood groups, and comorbidities. However, there was a significant difference in breakthrough infection rate among different types of vaccines (p = 0.001) and history of COVID-19 infection (p = 0.001). A total of 16 participants were hospitalized due to COVID-19 infection after vaccination for reasons such as dyspnea, abnormal imaging, or decreased oxygen saturation. No severe infection or death was observed in the studied population.

Conclusion

Vaccination prevented severe COVID-19 infection, although a high breakthrough infection rate was evident among Iranian medical students during the Delta variant’s peak. Vaccine effectiveness may be fragile during emerging new variants and in high-exposure settings. Moreover, adverse events are rare, and the benefits of vaccination outweigh the side effects. However, many limitations challenged this study, and the results should be cautious.
Listen, talk, repeat: women's journey through architecture and environmental consciousness
Authors: Papadopoulou Anna
Published: March 2024

Asserting that architecture is political is an acknowledgment that the design of form and space is founded on uneven relationships of power. At the same time, two states that are intrinsically apolitical—gender and climate—have become subject to intense socio-political polarization. While identifying these artificially imposed divides does not equate to their endorsement, the goal here is not to dismantle them. The aim is to elucidate how harnessing these differences helps a more sustainable built environment. This study delves into a practical approach, a mindset, embodied in the three-step process of “listen, talk, repeat”. This process frames a discourse on gender differences without victimization or criminalization of built space, societal systems, communities or their members. Derived from the social constructs of everyday life and domesticity, these three functions have been evoked by women of diverse backgrounds to navigate their everyday life and also to thrive therein. Deployment of this three-step mindset enables designers of all gender identities to mediate between theoretical space and practical applications, and to reposition socio-ecological sustainability as a fundamental aspect in salvaging a planet ravaged by extractivism and human ambition. Understanding how women have identified with this operational perspective reveals a rich tapestry of ideas, further organized by collective movements such as ecofeminism and the drive for resilience and sustainability. The narrative is illustrated by stories of women, and projects by women and by men, whose contribution has brought on unlikely paradigm shifts and, at times, decisive historical turning points.
Coral Reefs: A Story of Two Longtime Friends
Authors: Suescún-Bolívar Luis Parmenio , Thomé Patricia E. , Carabantes Natalia
Published: March 2024

Corals and tiny algae act as a team of engineers to form the building blocks needed to create the amazing coral reef ecosystem, which supports much of the marine life in the tropical oceans. These incredible ecosystems can extend for thousands of kilometers, like the Great Barrier Reef of Australia. Coral reefs would not exist without the help of the microscopic algae that associate with the coral and provide it with important nutrients for its survival. This article features the collaboration of amazing corals and their microalgae partners. You will learn about the processes that support the construction of coral reefs, their abilities to resist the threats that currently affect them (climate change, contamination), and the actions that can be taken to keep them safe and maintain the services that these ecosystems provide to other marine life.
What Can Robots Do For You?
Authors: Cagiltay Bengisu , Senft Emmanuel , Mutlu Bilge
Published: March 2024

You have probably seen movies in which robots do things like drive cars, deliver groceries, and fight space battles. But have you ever thought about what a robot could do for you? In the field of human-robot interaction, scientists study how robots can help people and what people think of robots. In this article, we meet three children in a classroom of the future and find out what robots do for them and how those robots know what to do. At school, Mia works with a robot friend who helps her learn a foreign language. Noah has special needs, and a robot in a hospital helps him learn about feelings and how to get along with other people. Last, Ari has a robot at home that helps her read, chats with her siblings, and helps her parents.
Scaling up: microbiome manipulation for climate change adaptation in large organic vineyards
Authors: Pino Carlos , Griffon Diego
Published: March 2024

Regenerative agriculture offers important solutions to the enormous challenges that the climate crisis poses on food production. However, there are doubts about the possibility of implementing many of these solutions in a particularly important sector: the large scale. This paper addresses the issue, presenting examples of large-scale vineyard soil microbiome manipulation in Chile. The South American country has strongly faced the effects of climate change during the last decade and the organic viticulture sector is actively seeking strategies to adapt to the new climatic reality. Here the results of 4 experiments under real production conditions are shown. The experiments were designed to assess the effects of adding various microbial consortia to the soil on key agronomic parameters. Successful as well as unsuccessful cases are presented, allowing discussion of some conditions under which the microbiome manipulation can be expected to have positive effects. It was found that under good management conditions, incorporating effective microorganisms has positive effects on important production parameters (yield, root and vegetative growth). However, when fields yields are trending downward for prolonged periods, the incorporation of effective microbial consortia (e.g., antagonistic fungi, nutrient-fixing and nutrient-solubilizing bacteria) does not have a positive effect on the vineyard trend immediately. Similarly, even in favorable conditions the positive effects cannot be expected to be expressed in the short term (i.e., in just a few months). Therefore, its use should be conceived as a long-term strategy, not as an immediate solution to urgent management problems.
Electrical impedance tomography in anaesthetised chickens (Gallus domesticus)
Authors: Wong Adrian M. , Lum Hei Y. , Musk Gabrielle C. , Hyndman Timothy H. , Waldmann Andreas D. , Monks Deborah J. , Bowden Ross S. , Mosing Martina
Published: March 2024

The applicability of electrical impedance tomography (EIT) in birds is unknown. This study aimed to evaluate the use of EIT in anaesthetised chickens in four recumbency positions. Four adult Hyline chickens were anaesthetised with isoflurane in oxygen, and intubated endotracheally for computed tomography (CT). A rubber belt was placed around the coelom caudal to the shoulder joint. A chicken-specific finite element (FE) model, which is essential to generate anatomically accurate functional EIT images for analysis, was constructed based on the CT images obtained at the belt level. Ten additional chickens were anaesthetised with the same protocol. An EIT electrode belt was placed at the same location. The chickens were breathing spontaneously and positioned in dorsal, ventral, right and left lateral recumbency in a randomised order. For each recumbency, raw EIT data were collected over 2 min after 13 min of stabilisation. The data were reconstructed into functional EIT images. EIT variables including tidal impedance variation (TIV), centre of ventilation right to left (CoVRL) and ventral to dorsal (CoVVD), right to left (RL) ratio, impedance change (ΔZ) and eight regional impedance changes including the dorsal, central-dorsal, central-ventral and ventral regions of the right and left regions were analysed. Four breathing patterns (BrP) were observed and categorised based on the expiratory curve. A linear mixed model was used to compare EIT variables between recumbencies. Fisher's exact test was used to compare the frequencies of breathing patterns for each recumbency. The ΔZ observed was synchronous to ventilation, and represented tidal volume of the cranial air sacs as confirmed by CT. Significant differences were found in CoVVD and regional impedance changes between dorsal and ventral recumbencies (P < 0.05), and in CoVRL, RL ratio and regional impedance changes between right and left recumbencies (P < 0.05), which suggested a tendency for the distribution of ventilation to shift towards non-dependent air sacs. No differences were found for TIV and respiratory rate between recumbencies. Recumbency had a significant effect on the frequencies of each of the four BrPs (P = 0.001). EIT can monitor the magnitude and distribution of ventilation of the cranial air sacs in different recumbencies in anaesthetised chickens.
A Preprocessing Method for Coronary Artery Stenosis Detection Based on Deep Learning
Authors: Li Yanjun , Yoshimura Takaaki , Horima Yuto , Sugimori Hiroyuki
Published: March 2024

The detection of coronary artery stenosis is one of the most important indicators for the diagnosis of coronary artery disease. However, stenosis in branch vessels is often difficult to detect using computer-aided systems and even radiologists because of several factors, such as imaging angle and contrast agent inhomogeneity. Traditional coronary artery stenosis localization algorithms often only detect aortic stenosis and ignore branch vessels that may also cause major health threats. Therefore, improving the localization of branch vessel stenosis in coronary angiographic images is a potential development property. In this study, we propose a preprocessing approach that combines vessel enhancement and image fusion as a prerequisite for deep learning. The sensitivity of the neural network to stenosis features is improved by enhancing the blurry features in coronary angiographic images. By validating five neural networks, such as YOLOv4 and R-FCN-Inceptionresnetv2, our proposed method can improve the performance of deep learning network applications on the images from six common imaging angles. The results showed that the proposed method is suitable as a preprocessing method for coronary angiographic image processing based on deep learning and can be used to amend the recognition ability of the deep model for fine vessel stenosis.
A Characterization of the Functions of OsCSN1 in the Control of Sheath Elongation and Height in Rice Plants under Red Light
Authors: Han Shining , Liu Yanxi , Bao Anor , Jiao Tongtong , Zeng Hua , Yue Weijie , Yin Le , Xu Miao , Lu Jingmei , Wu Ming , Guo Liquan
Published: March 2024

The COP9 signalosome (CSN) is a conserved protein complex, with CSN1 being one of the largest and most important subunits in the COP9 complex. To investigate the N-terminus function of OsCSN1, we edited the N-terminus of OsCSN1 and found that the mutant of OsCSN1 with 102 amino acids missing at the N-terminus showed insensitivity to red light in terms of the embryonic sheath, stem elongation, and main-root elongation. Moreover, the mutant was able to produce, develop, and bear fruit normally. The research results indicate that OsCSN1 is a negative regulator of stem elongation in rice seedlings regulated by red light. Under red-light treatment, OsCSN1 assembles into CSN, which degrades SLR1 through de NEDDylation, affecting PIL11 activity and ultimately inhibiting stem elongation. OsCSN1 also plays an important regulatory role in the inhibition of rice embryonic sheath elongation under red light. By regulating the degradation of SLR1 and PIL14 through the ubiquitin/26S protease pathway, the elongation of the embryonic sheath is ultimately inhibited. OsCSN1 forms a COP9 complex and is modified with RUB/NEDD8 of the E3 ligase of CUL1 to promote the degradation of SLR1 and PIL14, ultimately affecting the elongation of the embryonic sheath. The regulatory domain is located at the N-terminus of CSN1.
Contact Electrification of Liquid Droplets Impacting Living Plant Leaves
Authors: Hu Wei , Gao Zhouming , Dong Xiaoya , Chen Jian , Qiu Baijing
Published: March 2024

Contact electrification has attracted interest as a mechanism for generating electrical charges on surfaces. To explore the factors contributing to electrification by droplets impacting the leaf surface, high-speed image capture and current measurements were used to quantitatively characterize the electrical response under different droplet parameters and leaf surface conditions. Upon impact and rebound from the leaf surface, neutral droplets acquire a positive charge. While this electrification phenomenon has been observed previously, there has been limited understanding of the parameters influencing the extent of droplet charging. In this study, we examine the effects of four parameters (droplet size, impact velocity, droplet ion concentration, and various leaf surfaces) on the electrical response signal. The results indicate that this electrification phenomenon is contingent upon the droplet–leaf contact area and droplet ion concentration. We propose a theoretical model based on the electric double layer to elucidate the electrification process.
CASK Mediates Oxidative Stress-Induced Microglial Apoptosis-Inducing Factor-Independent Parthanatos Cell Death via Promoting PARP-1 Hyperactivation and Mitochondrial Dysfunction
Authors: Cheong Keith Jun Hao , Huang Duen-Yi , Sekar Ponarulselvam , Chen Rou Jhen , Cheng Irene Han-Juo , Chan Chi-Ming , Chen Yuan-Shen , Lin Wan-Wan
Published: March 2024

Calcium/calmodulin-dependent serine protein kinase (CASK) is a scaffold protein and plays critical roles in neuronal synaptic formation and brain development. Previously, CASK was shown to associate with EGFR to maintain the vulval cell differentiation in C. elegans. In this study, we explored the role of CASK in CHME3 microglial cells. We found that CASK silencing protects cells from H2O2-induced cell death by attenuating PARP-1 activation, mitochondrial membrane potential loss, reactive oxygen species production, and mitochondrial fission, but it increases oxidative phosphorylation. The PARP-1 inhibitor olaparib blocks H2O2-induced cell death, suggesting the death mode of parthanatos. CASK silencing also increases AKT activation but decreases AMPK activation under H2O2 treatment. Pharmacological data further indicate that both signaling changes contribute to cell protection. Different from the canonical parthanatos pathway, we did not observe the AIF translocation from mitochondria into the nucleus, suggesting a non-canonical AIF-independent parthanatos in H2O2-treated CHME3 cells. Moreover, we found that CASK silencing upregulates the EGFR gene and protein expression and increases H2O2-induced EGFR phosphorylation in CHME3 microglia. However, EGFR activation does not contribute to cell protection caused by CASK silencing. In conclusion, CASK plays a crucial role in microglial parthanatos upon H2O2 treatment via stimulation of PARP-1 and AMPK but the inhibition of AKT. These findings suggest that CASK might be an ideal therapeutic target for CNS disorders.
Screening for Metal-Chelating Activity in Potato Protein Hydrolysates Using Surface Plasmon Resonance and Peptidomics
Authors: Bjørlie Mads , Hartmann Julie Christina , Rasmussen Line Hyrup , Yesiltas Betül , Sørensen Ann-Dorit Moltke , Gregersen Echers Simon , Jacobsen Charlotte
Published: March 2024

Metal-catalyzed lipid oxidation is a major factor in food waste, as it reduces shelf life. Addressing this issue, our study investigates the potential of hydrolysates derived from potato protein, a by-product of potato starch production, as metal-chelating antioxidants. Through sequential enzymatic hydrolysis using alcalase or trypsin combined with Flavourzyme, we produced various hydrolysates, which were then fractionated using ultrafiltration. Using a combination of peptidomics and bioinformatics, we predicted the presence of metal-chelating and free radical-scavenging peptides across all hydrolysate fractions, with a trend indicating a higher content of antioxidant peptides in lower molecular weight fractions. To validate these predictions, we utilized surface plasmon resonance (SPR) and a 9-day emulsion storage experiment. While SPR demonstrated potential in identifying antioxidant activity, it faced challenges in differentiating between hydrolysate fractions due to significant standard errors. In the storage experiment, all hydrolysates showed lipid oxidation inhibition, though not as effectively as ethylenediaminetetraacetic acid (EDTA). Remarkably, one fraction (AF13) was not significantly different (p < 0.05) from EDTA in suppressing hexanal formation. These results highlight SPR and peptidomics/bioinformatics as promising yet limited methods for antioxidant screening. Importantly, this study reveals the potential of potato protein hydrolysates as antioxidants in food products, warranting further research.
Association between Environmental Neighbourhood Attributes and Self-Reported Health Outcomes among Urban Residents in Eastern Europe: A Cross-Sectional Study
Authors: Dėdelė Audrius , Chebotarova Yevheniia , Venclovienė Jonė , Miškinytė Auksė
Published: March 2024

Environmental perception is a complex issue that has significant impacts on public health. Despite limited research on subjective perceptions of the environment in various global contexts, including Ukraine, this study aimed to identify perceived environmental factors among urban residents and to assess associations with various health indicators. This is a cross-sectional study using data from the national survey carried out in 2017 in Ukraine. Data on demographic, behavioural, socioeconomic, and lifestyle risk factors were processed. A factor analysis was used to identify three environmental factors: outdoor infrastructure, environment, and safety and roads. The associations between these factors and health indicators were assessed using complex samples logistic regression models. The study showed that perceived environmental factors were significantly associated with body mass index, physical activity, stroke, stress, and harmful habits. Outdoor infrastructure emerged as the most important factor associated with health-related outcomes. Environmental neighbourhood attributes can significantly impact an individual’s health, highlighting the need for policies and interventions that promote healthy neighbourhood environments, including improvements in outdoor infrastructure, community resources, and safety measures. Our findings suggest that urban environmental improvements should be a priority in primary disease prevention, would positively impact public health, and would be beneficial to the community.
Energy-Efficient Internet of Drones Path-Planning Study Using Meta-Heuristic Algorithms
Authors: Ahmed Gamil , Sheltami Tarek , Ghaleb Mustafa , Hamdan Mosab , Mahmoud Ashraf , Yasar Ansar
Published: March 2024

The increasing popularity of unmanned aerial vehicles (UAVs), commonly known as drones, in various fields is primarily due to their agility, quick deployment, flexibility, and excellent mobility. Particularly, the Internet of Drones (IoD)—a networked UAV system—has gained broad-spectrum attention for its potential applications. However, threat-prone environments, characterized by obstacles, pose a challenge to the safety of drones. One of the key challenges in IoD formation is path planning, which involves determining optimal paths for all UAVs while avoiding obstacles and other constraints. Limited battery life is another challenge that limits the operation time of UAVs. To address these issues, drones require efficient collision avoidance and energy-efficient strategies for effective path planning. This study focuses on using meta-heuristic algorithms, recognized for their robust global optimization capabilities, to solve the UAV path-planning problem. We model the path-planning problem as an optimization problem that aims to minimize energy consumption while considering the threats posed by obstacles. Through extensive simulations, this research compares the effectiveness of particle swarm optimization (PSO), improved PSO (IPSO), comprehensively improved PSO (CIPSO), the artificial bee colony (ABC), and the genetic algorithm (GA) in optimizing the IoD’s path planning in obstacle-dense environments. Different performance metrics have been considered, such as path optimality, energy consumption, straight line rate (SLR), and relative percentage deviation (RPD). Moreover, a nondeterministic test is applied, and a one-way ANOVA test is obtained to validate the results for different algorithms. Results indicate IPSO’s superior performance in terms of IoD formation stability, convergence speed, and path length efficiency, albeit with a longer run time compared to PSO and ABC.
The Influence of the Wavelength of Laser Light on the Non-Contact Measurement of the Roughness of Shiny Cut Surfaces on Stainless Steel A304 Material
Authors: Ružbarský Juraj
Published: March 2024

The article is focused on the study of the effect of laser light with three wavelengths used in a laser profilometer for the measurement of selected roughness parameters of the shiny surface of stainless steel A304 material. The measured results were compared with the results we achieved with the reference contact roughness meter (SJ-400). The findings presented are relevant to the parameters of the experiment outlined within the article. In general, the obtained results make it possible to state that when measuring the roughness of shiny cut surfaces using non-contact laser profilometry, reflections of laser light occur. The relatively best results of measuring the parameters of the roughness of a shiny cut surface on the evaluated material (A304) were achieved by laser light with a wavelength of λ = 445 nm. In contrast, as the surface roughness of the cutting surface of the used material increased, the reflection of laser light decreased. Furthermore, we can state that the values of the roughness parameters Ra and Rz of the shiny surface measured by laser profilometry were several times higher than the values measured by the reference method. In contrast, the non-contact method of laser profilometry is not suitable for accurate measurements of the roughness parameters of shiny surfaces.
Improved Ionospheric Total Electron Content Maps over China Using Spatial Gridding Approach
Authors: Song Fucheng , Shi Shuangshuang
Published: March 2024

Precise regional ionospheric total electron content (TEC) models play a crucial role in correcting ionospheric delays for single-frequency receivers and studying variations in the Earth’s space environment. A particle swarm optimization neural network (PSO-NN)-based model for ionospheric TEC over China has been developed using a long-term (2008–2021) ground-based global positioning system (GPS), COSMIC, and Fengyun data under geomagnetic quiet conditions. In this study, a spatial gridding approach is utilized to propose an improved version of the PSO-NN model, named the PSO-NN-GRID. The root-mean-square error (RMSE) and mean absolute error (MAE) of the TECs estimated from the PSO-NN-GRID model on the test data set are 3.614 and 2.257 TECU, respectively, which are 7.5% and 5.5% smaller than those of the PSO-NN model. The improvements of the PSO-NN-GRID model over the PSO-NN model during the equinox, summer, and winter of 2015 are 0.4–22.1%, 0.1–12.8%, and 0.2–26.2%, respectively. Similarly, in 2019, the corresponding improvements are 0.5–13.6%, 0–10.1%, and 0–16.1%, respectively. The performance of the PSO-NN-GRID model is also verified under different solar activity conditions. The results reveal that the RMSEs for the TECs estimated by the PSO-NN-GRID model, with F10.7 values ranging within [0, 80), [80, 100), [100, 130), [130, 160), [160, 190), [190, 220), and [220, +), are, respectively, 1.0%, 2.8%, 4.7%, 5.5%, 10.1%, 9.1%, and 28.4% smaller than those calculated by the PSO-NN model.
Phosphatase and Tensin Homolog Inhibition in Proteolipid Protein 1-Expressing Cells Stimulates Neurogenesis and Gliogenesis in the Postnatal Enteric Nervous System
Authors: Woods Crystal , Flockton Amanda R. , Belkind-Gerson Jaime
Published: March 2024

Phosphatase and tensin homolog (Pten) is a key regulator of cell proliferation and a potential target to stimulate postnatal enteric neuro- and/or gliogenesis. To investigate this, we generated two tamoxifen-inducible Cre recombinase murine models in which Pten was conditionally ablated, (1) in glia (Plp1-expressing cells) and (2) in neurons (Calb2-expressing cells). Tamoxifen-treated adult (7–12 weeks of age; n = 4–15) mice were given DSS to induce colitis, EdU to monitor cell proliferation, and were evaluated at two timepoints: (1) early (3–4 days post-DSS) and (2) late (3–4 weeks post-DSS). We investigated gut motility and evaluated the enteric nervous system. Pten inhibition in Plp1-expressing cells elicited gliogenesis at baseline and post-DSS (early and late) in the colon, and neurogenesis post-DSS late in the proximal colon. They also exhibited an increased frequency of colonic migrating motor complexes (CMMC) and slower whole gut transit times. Pten inhibition in Calb2-expressing cells did not induce enteric neuro- or gliogenesis, and no alterations were detected in CMMC or whole gut transit times when compared to the control at baseline or post-DSS (early and late). Our results merit further research into Pten modulation where increased glia and/or slower intestinal transit times are desired (e.g., short-bowel syndrome and rapid-transit disorders).
Activity of Epsilon-poly-L-lysine against Multidrug-Resistant Pseudomonas aeruginosa and Klebsiella pneumoniae Isolates of Urinary Tract Infections
Authors: de Sousa Telma , Sabença Carolina , Ribeiro Miguel , Pino-Hurtado Mario , Torres Carmen , Hébraud Michel , Alves Olimpia , Sousa Sara , Costa Eliana , Igrejas Gilberto , Poeta Patrícia
Published: March 2024

Pseudomonas aeruginosa and Klebsiella pneumoniae are notorious for their resistance to antibiotics and propensity for biofilm formation, posing significant threats to human health. Epsilon-poly-L-lysine (ε-PL) emerges as a naturally occurring antimicrobial poly(amino acid), which positions it as a prospective agent for addressing challenges linked to multidrug resistance. ε-PL symbolizes a promising avenue in the pursuit of efficacious therapeutic strategies and warrants earnest consideration within the realm of clinical treatment. Thus, our objective was to determine the antibiotic susceptibility profiles of 38 selected P. aeruginosa and ESBL-producing K. pneumoniae clinical isolates and determine the ability of ε-PL to inhibit biofilm formation. After PCR analysis, detection of genes related to β-lactamases was observed among the selected isolates of P. aeruginosa [blaSPM (35.7%), blaKPC (35.7%), blaSHV (14.3%), blaCTX-M (14.3%), blaOXA (14.3%), blaTEM (7.1%), blaPER (7.1%), blaVIM (7.1%), and blaVIM-2 (7.1%)] and K. pneumoniae [blaCTX-M (91.7%), blaTEM (83.3%), blaKPC (16.7%), blaNDM (12.5%), and blaOXA (4.2%)]. The results of testing the activity of ε-PL against the clinical isolates showed relatively high minimum inhibitory concentrations (MICs) for the P. aeruginosa (range: 8–64 µg/mL) and K. pneumoniae isolates (range: 16–32 µg/mL). These results suggest the need for prior optimization of ε-PL concerning its viability as an alternative to antibiotics for treating infections caused by P. aeruginosa and K. pneumoniae of clinical origin. It is noteworthy that, in the context of a low antibiotic discovery rate, ε-PL could play a significant role in this quest, considering its low toxicity and the unlikely development of resistance. Upon exposure to ε-PL, P. aeruginosa and K. pneumoniae isolates exhibited a reduction in biofilm production, with ε-PL concentration showing an inverse relationship, particularly in isolates initially characterized as strong or moderate producers, indicating its potential as a natural antimicrobial agent with further research needed to elucidate optimal concentrations and application methods across different bacterial species. Further research is needed to optimize its use and explore its potential in various applications.
Genetic Monitoring of the Captive Population of the Critically Endangered Brazilian Merganser (Mergus octosetaceus)
Authors: Campos Davidson P. , Granger-Neto Henry P. , Santos-Júnior José E. , Buzatti Renata S. O. , Santos Fabrício R.
Published: March 2024

The Brazilian Merganser (Mergus octosetaceus) is one of the rarest birds in South America, and it is a critically endangered Anatidae species with an estimated population of less than 250 adult individuals in the Brazilian Cerrado. A captive population was established a few years ago at Zooparque Itatiba (São Paulo state) where 46 individuals were kept, and the founding population (progenitors derived from nature) was composed of 19 of the ex situ birds, derived from the four remaining localities with wild populations in Brazil. To characterize the genetic diversity and the genealogical relationships of the captive population, it is essential to conduct appropriate ex situ management and to assist future reintroduction projects. Thus, we have identified 425 SNPs by massively parallel sequencing of ddRAD libraries that allowed us to genotype individuals of the captive population. We observed a close relatedness between 70% of the captive population and founding individuals of Jalapão and Alto Paranaiba localities, indicating the need for supplementation with individuals from other areas of Canastra and Veadeiros. Even though many captives present a high level of inbreeding, we have identified some individuals with a high genetic value (less inbred) that can be selected for the breeding program to generate individuals for a future pilot reintroduction project.
Neurofilaments Light Chain in Neurodegenerative Dementias: A Review of Imaging Correlates
Authors: Gallingani Chiara , Carbone Chiara , Tondelli Manuela , Zamboni Giovanna
Published: March 2024

Neurofilaments light chain (NfLs) are currently recognized as a marker of axonal injury and degeneration. Their measurement in biological fluids has a promising role in the diagnosis, prognosis, and monitoring of the therapeutic response in neurological diseases, including neurodegenerative dementias. In recent years, their relationship with clinical phenotypes and measures of disease severity has been extensively studied. Here, we reviewed studies investigating the association between NfLs and imaging measures of grey matter (GM) and white matter (WM) damage in neurodegenerative dementias. We identified a large number of studies investigating this association in Alzheimer’s disease (AD) and disorders of the frontotemporal dementia (FTD) spectrum. Results were heterogeneous, possibly due to different methodological approaches—both in NfL measurements and imaging analyses—and inclusion criteria. However, a positive association between NfL levels and GM atrophy, WM microstructural disruption, glucose hypometabolism, and protein accumulation emerged invariably, confirming the role of NfLs as a reliable biomarker for neurodegenerative dementias, albeit not specific.
The Use of CGH Arrays for Identifying Copy Number Variations in Children with Autism Spectrum Disorder
Authors: Kucińska Agata , Hawuła Wanda , Rutkowska Lena , Wysocka Urszula , Kępczyński Łukasz , Piotrowicz Małgorzata , Chilarska Tatiana , Wieczorek-Cichecka Nina , Połatyńska Katarzyna , Przysło Łukasz , Gach Agnieszka
Published: March 2024

Autism spectrum disorders (ASDs) encompass a broad group of neurodevelopmental disorders with varied clinical symptoms, all being characterized by deficits in social communication and repetitive behavior. Although the etiology of ASD is heterogeneous, with many genes involved, a crucial role is believed to be played by copy number variants (CNVs). The present study examines the role of copy number variation in the development of isolated ASD, or ASD with additional clinical features, among a group of 180 patients ranging in age from two years and four months to 17 years and nine months. Samples were taken and subjected to array-based comparative genomic hybridization (aCGH), the gold standard in detecting gains or losses in the genome, using a 4 × 180 CytoSure Autism Research Array, with a resolution of around 75 kb. The results indicated the presence of nine pathogenic and six likely pathogenic imbalances, and 20 variants of uncertain significance (VUSs) among the group. Relevant variants were more prevalent in patients with ASD and additional clinical features. Twelve of the detected variants, four of which were probably pathogenic, would not have been identified using the routine 8 × 60 k microarray. These results confirm the value of microarrays in ASD diagnostics and highlight the need for dedicated tools.
Stress Control in Older People through Healing Garden Activities
Authors: Kim Sun-Hee , Seo Joo-Bong , Ryu Byung-Yeol
Published: March 2024

This study, conducted on a group older than 60-years-of-age, sought to verify if healing garden activities control stress in older people. The experimental group performed garden activities once a week for 12 weeks, for 2 h each day, and the control group continued their daily lives. Each group’s cumulative stress at the beginning and end, along with total power (TP), the standard deviation of the normal-to-normal interval (SDNN), and the root mean square differences of successive R–R intervals (RMSSD), were measured using u-Bio MACPA, a stress index meter. The analysis showed that the experimental group had a statistically significant decrease in cumulative stress, compared to the control group; and also that TP significantly increased, compared to the control group. The SDNN and RMSSD of the experimental group increased, and decreased in the control group, but the changes were not statistically significant. The fact that cumulative stress decreased and stress evaluation indicators increased shows that daily stress can be controlled through healing garden activities. However, due to the small number of participants in the experiment, its ability to be generalized to all elderly people is subject to a number of limitations. Nevertheless, I think it is meaningful that the finding that garden activities are significant in mediating stress in the elderly was verified using a scientific measurement instrument. Future studies should explore the healing effectiveness of gardens in other age groups.
Evaluation of the Cost-Effectiveness of Evidence-Based Interventions to Increase Female Breast and Cervical Cancer Screens: A Systematic Review
Authors: Phillips Victoria , Franco Montoya Daniela , Adams E. Kathleen
Published: March 2024

Purpose: To systematically review published cost-effectiveness analyses of Evidence-Based Interventions (EBIs) recommended by the United States Community Preventive Services Task Force (CPSTF) to increase breast and cervical cancer screening. Methods: We searched PubMed and Embase for prospective cost-effectiveness evaluations of EBIs for breast and cervical cancer screening since 1999. We reviewed studies according to the Consolidated Health Economic Evaluation Reporting Standards (CHEERS) and compared the incremental cost-effectiveness ratio (ICERs), defined as cost per additional woman screened, adjusted to 2021 USD, within and across EBIs by cancer type. Results: We identified eleven studies meeting our review criteria: nine were breast cancer-focused, one breast and cervical cancer combined, and one cervical only, which together reported twenty-four cost-effectiveness assessments of outreach programs spanning eight EBIs. One-on-one education programs were the most common EBI evaluated. The average ICER across breast cancer studies was USD 545 (standard deviation [SD] = USD 729.3), while that for cervical cancer studies was USD 197 (SD = 186.6. Provider reminder/recall systems for women already linked to formal care were the most cost-effective, with an average ICERs of USD 41.3 and USD 10.6 for breast and cervical cancer, respectively. Conclusions: Variability in ICERs across and within EBIs reflect the population studied, the specific EBI, and study settings, and was relatively high. ICER estimate uncertainty and the potential for program replicability in other settings and with other populations were not addressed. Given these limitations, using existing cost-effectiveness estimates to inform program funding allocations is not warranted at this time. Additional research is needed on outreach programs for cervical cancer and those which serve minority populations for either of the female cancer screens.