bluuebunny/crossref_metadata_2025 huggingface dataset:
Functional interactions of neurogenic genes of Drosophila melanogaster.
Authors: de-la-Concha A , Dietrich U , Weigel D , Campos-Ortega J A
Published: January 2021

The neurogenic genes of Drosophila melanogaster are involved in the decision of ectodermal cells to take on a neural or an epidermal fate. We present evidence in support of the notion that six of the neurogenic genes are functionally related. We studied the phenotype of embryos lacking one of the neurogenic genes in the presence of an increased dosage of the wild-type allele of another neurogenic gene. Our analysis also included the Hairless locus, whose function is related to that of the neurogenic genes, as well as to many other genes. The effects observed were asymmetric in that triploidy for a given gene modified the phenotype of loss of the function of another gene, but triploidy of the latter gene did not modify the phenotype of loss of the function of the former gene. These asymmetries allowed us to establish a polarity of gene interactions, as well as to order the genes according to the assumed ability of some of them to modify the activity of others. In this sequence, almondex is the first link and Enhancer of split the last one. Our evidence suggests that the function of big brain is independent of the function of the other six. The consequences of this arrangement for the commitment of ectodermal cells are discussed.
Spontaneous formation of compound X chromosomes in Drosophila melanogaster.
Authors: Morrison R J , Raymond J D , Zunt J R , Lim J K , Simmons M J
Published: January 2021

Males carrying different X chromosomes were tested for the ability to produce daughters with attached-X chromosomes. This ability is characteristic of males carrying an X chromosome derived from 59b-z, a multiply marked X chromosome, and is especially pronounced in males carrying the unstable 59b-z chromosomes Uc and Uc-lr. Recombination experiments with one of the Uc-lr chromosomes showed that the formation of compound chromosomes depends on two widely separated segments. One of these is proximal to the forked locus and is probably proximal to the carnation locus. This segment may contain the actual site of chromosome attachment. The other essential segment lies between the crossveinless and vermilion loci and may contain multiple factors that influence the attachment process.
Analysis of the mechanism for reversion of a disrupted gene.
Authors: Schiestl R H , Igarashi S , Hastings P J
Published: January 2021

A positive selection system for intrachromosomal recombination in Saccharomyces cerevisiae has been developed. This was achieved by integration of a plasmid containing an internal fragment of the HIS3 gene into its chromosomal location. This resulted in two copies of the HIS3 gene one with a terminal deletion at the 3' end and the other with a terminal deletion at the 5' end. Reversion of the gene disruption could be brought about by plasmid excision, unequal sister chromatid exchange or sister chromatid conversion. The purpose of this study was to define the mechanisms involved in reversion of the gene disruption. The frequency of plasmid excision could be determined by placing a yeast sequence bearing an origin of replication onto the plasmid that was subsequently integrated into the yeast genome. Unequal sister chromatid exchange and conversion could be distinguished by determining the nature of the reciprocal product by Southern blotting. The results indicate that reversion might occur mainly by conversion between sister chromatids. This is because the frequency of plasmid excision was about two orders of magnitude lower than the overall frequency of reversion and no reciprocal product indicative of sister chromatid exchange was found. The findings of this presentation suggest that conversion might be an important mechanism for recombination of sister chromatids and possibly for repair of damaged DNA in S or G2.
Genetic and physical analysis of the M26 recombination hotspot of Schizosaccharomyces pombe.
Authors: Ponticelli A S , Sena E P , Smith G R
Published: January 2021

The ade6-M26 mutation of Schizosaccharomyces pombe has previously been reported to stimulate ade6 intragenic meiotic recombination. We report here that the ade6-M26 mutation is a single G----T nucleotide change, that M26 stimulated recombination within ade6 but not at other distinct loci, and that M26 stimulated meiotic but not mitotic recombination. In addition, M26 stimulated recombination within ade6 when M26 is homozygous; this result demonstrates that a base-pair mismatch at the M26 site was not required for the stimulation. These results are consistent with the ade6-M26 mutation creating a meiotic recombination initiation site.
Naturally occurring variation in the restriction map of the amy region of Drosophila melanogaster.
Authors: Langley C H , Shrimpton A E , Yamazaki T , Miyashita N , Matsuo Y , Aquadro C F
Published: January 2021

The restriction maps of 85 alleles of the Amy region of Drosophila melanogaster from natural populations were surveyed. A subset of these were also scored for allozyme phenotype and adult enzyme activity of alpha-amylase. Large insertions were found in 12% of the alleles in a 15-kb region surrounding the two transcriptional units of the duplicated Amy locus. The low frequencies at which each of these large insertions were found are consistent with earlier reports of variation in other loci. Four small deletions were found in the region 5' to the Amy genes. Each was also rare in the population. Restriction site variation provided an estimate of per nucleotide heterozygosity of 0.006. Several statistically significant linkage disequilibria were observed between four polymorphic restriction sites and the allozymes. Adult alpha-amylase activity was correlated with the allozymes and with the polymorphism at one restriction site close to the transcriptional units.
Evolution of the autosomal chorion locus in Drosophila. I. General organization of the locus and sequence comparisons of genes s15 and s19 in evolutionary distant species.
Authors: Martínez-Cruzado J C , Swimmer C , Fenerjian M G , Kafatos F C
Published: January 2021

We have isolated clones corresponding to the autosomal chorion locus of Drosophila melanogaster, from two distantly (D. virilis and D. grimshawi) and one closely (D. subobscura) related species. In all the species the locus is unique within the genome and encompasses the same four chorion genes and an adjacent nonchorion gene, in the same order. In all species the locus specifically amplifies in the ovary, as in D. melanogaster. We present the nucleotide sequences of DNA segments that total 8.3 kb in length and include gene s15-1 from D. subobscura, D. virilis, and D. grimshawi as well as gene s19-1 from D. subobscura and D. grimshawi. They show clearly nonuniform rates of divergence, both within and outside the limits of the genes. Highlighted by a background of extensive sequence divergence elsewhere in the extragenic region, highly conserved elements are observed in the 5' flanking DNA and might represent regulatory elements.
Double-stranded gap repair of DNA by gene conversion in Escherichia coli.
Authors: Kobayashi I , Takahashi N
Published: January 2021

We demonstrated repair of a double-stranded DNA gap through gene conversion by a homologous DNA sequence in Escherichia coli. We made a double-stranded gap in one of the two regions of homology in an inverted orientation on a plasmid DNA molecule and introduced it into an E. coli strain which has the RecE system of recombination (genotype; sbcA23 recB21 recC22). We detected repair products by genetic selection. The repair products were those expected by the double-strand-gap repair model. Gene conversion was frequently accompanied by crossing over of the flanking sequences as in eukaryotes. This double-strand gap repair mechanism can explain plasmid recombination in the absence of an artificial double-stranded break reported in a companion study by Yamamoto et al.
The rosy region of Drosophila melanogaster and Drosophila simulans. I. Contrasting levels of naturally occurring DNA restriction map variation and divergence.
Authors: Aquadro C F , Lado K M , Noon W A
Published: January 2021

A 40-kb region around the rosy and snake loci was analyzed for restriction map variation among 60 lines of Drosophila melanogaster and 30 lines of Drosophila simulans collected together at a single locality in Raleigh, North Carolina. DNA sequence variation in D. simulans was estimated to be 6.3 times greater than in D. melanogaster (heterozygosities per nucleotide of 1.9% vs. 0.3%). This result stands in marked contrast to results of studies of phenotypic variation including proteins (allozymes), morphology and chromosome arrangements which are generally less variable and less geographically differentiated in D. simulans. Intraspecific polymorphism is not distributed uniformly over the 40-kb region. The level of heterozygosity per nucleotide varies more than 12-fold across the region in D. simulans, being highest over the hsc2 gene. Similar, though less extreme, variation in heterozygosity is also observed in D. melanogaster. Average interspecific divergence (corrected for intraspecific polymorphism) averaged 3.8%. The pattern of interspecific divergence over the 40-kb region shows some disparities with the spatial distribution of intraspecific variation, but is generally consistent with selective neutrality predictions: the most polymorphic regions within species are generally the most divergent between species. Sequence-length polymorphism is observed for D. melanogaster to be at levels comparable to other gene regions in this species. In contrast, no sequence length variation was observed among D. simulans chromosomes (limit of resolution approximately 100 bp). These data indicate that transposable elements play at best a minor role in the generation of naturally occurring genetic variation in D. simulans compared to D. melanogaster. We hypothesize that differences in species effective population size are the major determinant of the contrasting levels and patterns of DNA sequence and insertion/deletion variation that we report here and the patterns of allozyme and morphological variation and differentiation reported by other workers for these two species.
Genetic evidence that the sans fille locus is involved in Drosophila sex determination.
Authors: Oliver B , Perrimon N , Mahowald A P
Published: January 2021

Females homozygous for sans fille1621 (= fs(1)1621) have an abnormal germ line. Instead of producing eggs, the germ-line cells proliferate forming ovarian tumors or excessive numbers of nurse cells. The Sex-lethal gene product(s) regulate the branch point of the dosage compensation and sex determination pathways in the soma. The role of Sex-lethal in the germ line is not clear but the germ line of females homozygous for female sterile Sex-lethal alleles or germ-line clones of loss-of-function alleles are characterized by ovarian tumors. Females heterozygous for sans fille1621 or Sex-lethal are phenotypically wild type with respect to viability and fertility but females trans-heterozygous for sans fille1621 and Sex-lethal show ovarian tumors, somatic sexual transformations, and greatly reduced viability.
Genetic analysis of mouse t haplotypes using mutations induced by ethylnitrosourea mutagenesis: the order of T and qk is inverted in t mutants.
Authors: Justice M J , Bode V C
Published: January 2021

The t region of mouse chromosome 17 exhibits recombination suppression with wild-type chromatin. However, the region has resisted classical genetic dissection because of a lack of defined variants. Mutations induced by N-ethyl-N-nitrosourea (ENU) at the Brachyury (T), quaking (qk), and tufted (tf) loci of the mouse tw5 haplotype have now allowed the analysis of crossovers between two complete t haplotypes. A classical breeding analysis of the complete t haplotypes, tw5 and t12, utilizing the newly induced markers, reveals two inversions in t chromatin: one involving T and qk, and one involving tf and the H-2 complex. Moreover, the recombination frequency between the loci of T and qk is reduced compared to the frequency reported in normal chromatin. These two inversions are a sufficient explanation for the recombination inhibition with normal chromatin exhibited by t haplotypes isolated from the wild. Furthermore, the reduced recombination frequency between T and qk may indicate that the proximal gene rearrangement is not a simple inversion.
Maintaining Sense of Purpose Following Health Adversity in Older Adulthood: A Propensity Score Matching Examination
Authors: Hill Patrick L , Beck Emorie D , Jackson Joshua J
Published: January 2021

Objectives

Research has demonstrated sense of purpose predicts better health in older adulthood. However, work is limited with respect to understanding how experiencing a health event or illness diagnosis impacts older adults’ sense of purpose.

Method

The current study employed a propensity score matching approach to compare older adults who did or did not experience an adverse health event on changes in sense of purpose across 3 waves of the Health and Retirement Study. Sense of purpose was assessed at each wave, and changes were compared between people who did versus did not experience one of 7 diagnoses and health events.

Results

When propensity score matching was employed, no differences in trajectories of change for sense of purpose were found with respect to all 7 events. Individual differences in trajectories, however, were evidenced across groups.

Discussion

The current findings suggest that even when health events impact older adults’ physical functioning or place limitations on their activity, they may hold little ramifications for their sense of purpose. Future research should consider this potential for resilience, focusing on how adults compensate for losses.
Revisiting the Role of Gender and Marital Status as Risk Factors for Nursing Home Entry
Authors: Casanova Maria
Published: January 2021

Objective

To study the role of gender and marital status as risk factors for nursing home entry in the United States.

Method

The paper uses data from the Health and Retirement Study, a nationally representative survey of the older population in the United States. Multivariate logit models of the risk of nursing home entry over a 2-year follow-up period were estimated for noninstitutionalized individuals over the age of 65. A multiple imputation procedure was used to explore the sensitivity of the results to alternative assumptions about the data-generating process of missing outcome values.

Results

In an analysis based on complete observations, women exhibited the same risk of nursing home entry as men (risk ratio [RR] = 1.01; CI: 0.90, 1.13). However, after expanding the sample to include information on nursing home use for individuals who died during the follow-up period, women were found to have a statistically lower risk of nursing home entry (RR = 0.85; CI: 0.79, 0.92). The latter result was robust to alternative assumptions about the nature of missing data. The type of sample used in the analysis did not affect the conclusions regarding the role of marital status. Divorced and widowed individuals were found to be at higher risk of nursing home admissions than married individuals in all specifications.

Discussion

The findings clarify the role of gender as a predictor of nursing home admissions and may provide useful prognostic information for clinicians and caregivers regarding nursing home entry risk. The study also sheds light on how conclusions about predictors of nursing home risk obtained from prospective studies with long follow-up periods can be affected by the treatment of missing outcomes due to death or attritions.
Knowledge Exchanges and Decision-Making Within Hospital Dementia Care Triads: An Ethnographic Study
Authors: Kelley Rachael , Godfrey Mary , Young John
Published: January 2021

Background and Objectives

Important decisions about the future care of people living with dementia are routinely made in hospitals. Very little is known about how the care needs of hospitalized people with dementia are understood, or how the perspectives of the person, families, and staff intersect to inform decision-making. This study explores how the care needs of people with dementia are understood by the person, their family, and hospital staff (the care triad), and how these perspectives shape decision-making.

Research Design and Methods

Ethnographic data were collected from 2 care-of-older-people general hospital wards via observations, conversations, and interviews with people with dementia, families, and staff. In total, 400 hr of observation and 46 interviews were conducted across two 7- to 9-month periods.

Results

The person’s care needs were often understood differently between and within arms of the care triad. A lack of consistent engagement with families and people with dementia reduced opportunities to recognize and integrate this range of views, leading to delays or difficulties in decision-making. People with dementia, particularly those lacking capacity, were most likely to have their perspectives overlooked.

Discussion and Implications

Early engagement with people with dementia and their families is required to ensure that all perspectives on the person’s current and future care needs are understood and represented during decision-making. Particular attention should be paid to involving people living with dementia in discussions and decisions about their care, and to the assessment and involvement of people who may lack capacity.
Patient satisfaction with outpatient care in China: a comparison of public secondary and tertiary hospitals
Authors: Yan Jingjing , Yao Jiansen , Zhao Dahai
Published: January 2021

Objective

The aims of this study were to investigate and compare patient satisfaction with outpatient care in public secondary and tertiary hospitals in China and to explore the factors affecting patient satisfaction for improving the quality of outpatient care in public hospitals.

Methods

This cross-sectional study comprised a sample survey of 11 097 adults in 31 provincial cities in China from February to April 2018. A pretested structured questionnaire was used to collect outpatient experience data through a computer-assisted telephone interviewing system. Patient satisfaction was assessed using nine questions answered on a 4-point Likert scale. Multivariate regression models were employed to examine the relationships of patient satisfaction with outpatient services and healthcare provider level and to identify the factors associated with satisfaction.

Results

Patient’s overall satisfaction score with outpatient care was 27.3 (SD = 3.8), with lower scores observed in tertiary hospitals than in secondary hospitals (27.3 vs. 27.6, P < 0.05). The domain with the highest satisfaction was ‘consulting environment’, and the domain with the lowest satisfaction was ‘patient waiting time in the hospital’. Patients who went to tertiary hospitals reported lower satisfaction in ‘patient waiting time in the hospital’, ‘medical expenses’, ‘patient length of treatment time’ and ‘attitudes of other health workers’ than patients who went to secondary hospitals (P < 0.05). In secondary hospitals, no significant difference in patient satisfaction was observed between different sociodemographic categories (P > 0.05). In tertiary hospitals, female and single respondents were more likely to have higher satisfaction (P < 0.05), whereas respondents with high school or junior college degrees were more likely to have lower satisfaction (P < 0.05).

Conclusion

The aforementioned results suggested that tertiary hospitals face larger challenges in patient satisfaction with outpatient care than secondary hospitals. Measures must be adopted to improve patient satisfaction with outpatient care in future healthcare reforms. Patient waiting time, medical expenses and treatment duration especially require improvements in tertiary hospitals.
An evidence-based oxytocin protocol for the third stage of labor to improve hemorrhage outcomes
Authors: Gonzalez Sara C , Budge Jeffrey , Butler Patricia D , Higgs Maureen , Whiting Judd , Lutgendorf Monica A
Published: January 2021

Objective

To assess the use of an evidence-based oxytocin protocol for management of the third stage of labor to decrease non-beneficial clinical variation and improve clinical outcomes.

Design

This is a cohort study of pregnant patients delivering before implementation of an evidence-based oxytocin protocol compared to patients delivering after implementation of an evidence-based oxytocin protocol.

Setting

A level III maternal care referral hospital with an average delivery volume of approximately 3000 deliveries.

Participants

Pregnant patients delivering over a 60-month period from January 2013 to December 2017.

Intervention

An evidence-based oxytocin protocol: 3 units of oxytocin administered over 3 minutes, with a second 3-unit bolus if inadequate tone, then oxytocin infusion at 18 units/hour × 1 hour and then 3.6 units/hour for 3 hours.

Main outcome measures

Postpartum hemorrhage (PPH) rate (EBL ≥500 ml for vaginal and ≥1000 ml for cesarean).

Results

Data from 14 603 deliveries were analyzed, 8408 pre-protocol and 6195 post-protocol. We demonstrated a significant decrease in PPH from 5.2% to 2.9% (P < 0.001) and a small but non-significant increase in the transfusion rate from 1.8% to 2.3% (P = 0.11).

Conclusion

A standardized oxytocin infusion protocol in the third stage of labor resulted in a significant decrease in PPH for both vaginal and cesarean deliveries.
Quantification of abdominal fat from computed tomography using deep learning and its association with electronic health records in an academic biobank
Authors: MacLean Matthew T , Jehangir Qasim , Vujkovic Marijana , Ko Yi-An , Litt Harold , Borthakur Arijitt , Sagreiya Hersh , Rosen Mark , Mankoff David A , Schnall Mitchell D , Shou Haochang , Chirinos Julio , Damrauer Scott M , Torigian Drew A , Carr Rotonya , Rader Daniel J , Witschey Walter R
Published: January 2021

Objective

The objective was to develop a fully automated algorithm for abdominal fat segmentation and to deploy this method at scale in an academic biobank.

Materials and Methods

We built a fully automated image curation and labeling technique using deep learning and distributive computing to identify subcutaneous and visceral abdominal fat compartments from 52,844 computed tomography scans in 13,502 patients in the Penn Medicine Biobank (PMBB). A classification network identified the inferior and superior borders of the abdomen, and a segmentation network differentiated visceral and subcutaneous fat. Following technical evaluation of our method, we conducted studies to validate known relationships with visceral and subcutaneous fat.

Results

When compared with 100 manually annotated cases, the classification network was on average within one 5-mm slice for both the superior (0.4 ± 1.1 slice) and inferior (0.4 ± 0.6 slice) borders. The segmentation network also demonstrated excellent performance with intraclass correlation coefficients of 1.00 (P < 2 × 10-16) for subcutaneous and 1.00 (P < 2 × 10-16) for visceral fat on 100 testing cases. We performed integrative analyses of abdominal fat with the phenome extracted from the electronic health record and found highly significant associations with diabetes mellitus, hypertension, and renal failure, among other phenotypes.

Conclusions

This work presents a fully automated and highly accurate method for the quantification of abdominal fat that can be applied to routine clinical imaging studies to fuel translational scientific discovery.
Genome-wide analysis of chromatin structure changes upon MyoD binding in proliferative myoblasts during the cell cycle
Authors: Wu Qianmei , Fujii Takeru , Harada Akihito , Tomimatsu Kosuke , Miyawaki-Kuwakado Atsuko , Fujita Masatoshi , Maehara Kazumitsu , Ohkawa Yasuyuki
Published: January 2021

MyoD, a myogenic differentiation protein, has been studied for its critical role in skeletal muscle differentiation. MyoD-expressing myoblasts have a potency to be differentiated with proliferation of ectopic cells. However, little is known about the effect on chromatin structure of MyoD binding in proliferative myoblasts. In this study, we evaluated the chromatin structure around MyoD-bound genome regions during the cell cycle by chromatin immunoprecipitation sequencing. Genome-wide analysis of histone modifications was performed in proliferative mouse C2C12 myoblasts during three phases (G1, S, G2/M) of the cell cycle. We found that MyoD-bound genome regions had elevated levels of active histone modifications, such as H3K4me1/2/3 and H3K27ac, compared with MyoD-unbound genome regions during the cell cycle. We also demonstrated that the elevated H3K4me2/3 modification level was maintained during the cell cycle, whereas the H3K27ac and H3K4me1 modification levels decreased to the same level as MyoD-unbound genome regions during the later phases. Immunoblot analysis revealed that MyoD abundance was high in the G1 phase then decreased in the S and G2/M phases. Our results suggest that MyoD binding formed selective epigenetic memories with H3K4me2/3 during the cell cycle in addition to myogenic gene induction via active chromatin formation coupled with transcription.
Reproductive Strategies That May Facilitate Invasion Success: Evidence From a Spider Mite
Authors: Zhou Peng , He Xiong Zhao , Chen Chen , Wang Qiao
Published: January 2021

With significant surge of international trade in recent decades, increasingly more arthropod species have become established outside their natural range of distribution, causing enormous damage in their novel habitats. However, whether a species can colonize its new environment depends on its ability to overcome various barriers which may result in establishment failure, such as inbreeding depression and difficulty to find mates. Here, we used a haplodiploid pest, Tetranychus ludeni Zacher (Acari: Tetranychidae), which is native to Europe but now cosmopolitan, to investigate whether its reproductive strategies have facilitated its invasion success, providing knowledge to develop programs for prediction and management of biological invasions. We show that inbreeding had no negative influence on female reproductive outputs and longevity over 11 successive generations, allowing mother-son and brother-sister mating to occur at the invasion front without adverse consequences in fitness. Virgin females produced maximum number of sons in their early life to ensure subsequent mother-son mating but later saved resources to prolong longevity for potential future mating. Females maximized their resource allocation to egg production immediately after mating to secure production of maximum number of both daughters and sons as early as possible. Furthermore, mated females with mating delay increased proportion of daughters in offspring produced to compensate the loss of production of daughters during their virgin life. We suggest that the lack of inbreeding depression in successive generations and the ability to adjust resource allocations depending whether and when mating occurs may be the key features that have facilitated its invasion success.
Detection of Novel Spotted Fever Group Rickettsiae (Rickettsiales: Rickettsiaceae) in Ticks (Acari: Ixodidae) in Southwestern China
Authors: Wang Qian , Guo Wen-Bin , Pan Yu-Sheng , Jiang Bao-Gui , Du Chun-Hong , Que Teng-Cheng , Zhan Lin , Wu Jia-Hong , Yu Ming-Hui , Cui Xiao-Ming , Zhao Lin , Xu Da-Li , Xia Luo-Yuan , Ye Run-Ze , Li Jie , Li Lian-Feng , Wei Wei , Zhou Yu-Hao , Jiang Jia-Fu , Jia Na , Cao Wu-Chun
Published: January 2021

Spotted fever group rickettsiae, mainly maintained and transmitted by ticks, are important etiological agents of (re)emerging zoonotic diseases worldwide. It is of great significance to investigate spotted fever group rickettsiae in ticks in different areas for the prevention and control of rickettsioses. In this study, a total of 305 ticks were collected from wild and domestic animals in Chongqing, Guizhou, Yunnan, and Guangxi provinces of southwestern China during 2017–2019 and examined for the presence of spotted fever group rickettsiae by PCR with primers targeting the partial gltA, ompA, rrs, and htrA genes. Results showed that two spotted fever group rickettsiae species, including the pathogenic Candidatus Rickettsia jingxinensis (Rickettsiales: Rickettsiaceae) and a potential novel species Rickettsia sp. sw (Rickettsiales: Rickettsiaceae), were identified. The Ca. R. jingxinensis sequences were recovered from Rhipicephalus microplus (Ixodida: Ixodidae) and Haemaphysalis longicornis (Ixodida: Ixodidae) ticks and phylogenetically clustered with previous Ca. R. jingxinensis, Ca. R. longicornii (Rickettsiales: Rickettsiaceae), and Rickettsia sp. XY118 (Rickettsiales: Rickettsiaceae) strains. Rickettsia sp. sw was detected in Amblyomma geoemydae (Ixodida: Ixodidae) and Rh. microplus. Interestingly, as far as we know, this was the first report of Rickettsia (Rickettsiales: Rickettsiaceae) in A. geoemydae. Phylogenetic analyses indicated that this potential novel species was closely related to R. aeschlimannii (Rickettsiales: Rickettsiaceae) with gltA and ompA genes and grouped in a cluster composed of R. montanensis (Rickettsiales: Rickettsiaceae), R. raoultii (Rickettsiales: Rickettsiaceae), R. aeschlimannii, R. massiliae (Rickettsiales: Rickettsiaceae), and R. rhipicephali (Rickettsiales: Rickettsiaceae) with htrA, while formed a separate clade with rrs. The pathogenicity of Rickettsia sp. sw should be further confirmed. These results expand the knowledge of the geographical distribution and vector distribution of spotted fever group rickettsiae in China and are useful for assessing the potential public health risk.
EXPOSURE DOSE INDEX BASED ON NOISE FACTOR ANALYSIS IN DIGITAL MAMMOGRAPHY: VERIFICATION USING DIRECT-TYPE FLAT-PANEL DETECTOR SYSTEM
Authors: Maruyama Sho
Published: January 2021

In this study, the development of a reasonable index of optimized exposure dose is attempted. Using a direct-type flat-panel system, noise factors contained in the image are analyzed based on the relative standard deviation method, and it is verified that the proposed index conforms with the appropriate standards regarding minimum exposure dose. The findings indicate that Poisson noise is dominant in the general clinical dose range; this noise fraction formed 90% of the total noise in the system considered in this study. Considering the results of previous surveys, it is estimated that the point at which the content rate of the Poisson noise starts to decrease can be considered as the lower limit, below which the dose cannot be reduced further without compromising on image quality. As the ‘index’ obtained by this method can be easily measured, it is useful as a quantitative indicator for dose determination and optimization.
Is Music Therapy Effective in Cabin Fever Recovery?
Authors: Situmorang Dominikus David Biondi
Published: January 2021

Objectives:

During the coronavirus disease (COVID-19) outbreak, there is an unhealthy psychological symptom in most people worldwide, namely cabin fever. Quite a few people in this world do not know the benefits of music therapy in helping to prevent and overcome cabin fever.

Methods:

Through this opinion “piece,” the author aims to discuss tips to prevent and overcome cabin fever through several studies with music therapy.

Conclusion:

The author proposes that everyone can use music therapy in preventing and overcoming cabin fever.
Dairy Fat Intake, Plasma Pentadecanoic Acid, and Plasma Iso‐heptadecanoic Acid Are Inversely Associated With Liver Fat in Children
Authors: Sawh Mary Catherine , Wallace Martina , Shapiro Emma , Goyal Nidhi P. , Newton Kimberly P. , Yu Elizabeth L. , Bross Craig , Durelle Janis , Knott Cynthia , Gangoiti Jon A. , Barshop Bruce A. , Gengatharan Jivani M. , Meurs Noah , Schlein Alexandra , Middleton Michael S. , Sirlin Claude B. , Metallo Christian M. , Schwimmer Jeffrey B.
Published: January 2021

Objectives:

We sought to evaluate the relevance of pediatric dairy fat recommendations for children at risk for nonalcoholic fatty liver disease (NAFLD) by studying the association between dairy fat intake and the amount of liver fat. The effects of dairy fat may be mediated by odd chain fatty acids (OCFA), such as pentadecanoic acid (C15:0), and monomethyl branched chain fatty acids (BCFA), such as iso‐heptadecanoic acid (iso‐C17:0). Therefore, we also evaluated the association between plasma levels of OCFA and BCFA with the amount of liver fat.

Methods:

Observational, cross‐sectional, community‐based sample of 237 children ages 8 to 17. Dairy fat intake was assessed by 3 24‐hour dietary recalls. Plasma fatty acids were measured by gas chromatography‐mass spectrometry. Main outcome was hepatic steatosis measured by whole liver magnetic resonance imaging proton density fat fraction (MRI‐PDFF).

Results:

Median dairy fat intake was 10.6 grams/day (range 0.0‐‐44.5 g/day). Median liver MRI‐PDFF was 4.5% (range 0.9%–45.1%). Dairy fat intake was inversely correlated with liver MRI‐PDFF (r = −0.162; P = .012). In multivariable log linear regression, plasma C15:0 and iso‐C17:0 were inverse predictors of liver MRI‐PDFF (B = −0.247, P = 0.048; and B = −0.234, P = 0.009).

Conclusions:

Dairy fat intake, plasma C15:0, and plasma iso‐C17:0 were inversely correlated with hepatic steatosis in children. These hypothesis‐generating findings should be tested through clinical trials to better inform dietary guidelines.
Spectrum of Clinicopathologic Findings in COVID-19-induced Skin Lesions
Authors: Garrido Ruiz Maria C. , Santos-Briz Ángel , Santos-Briz Ángel , Sánchez Alba , Alonso-Riaño Marina , Burgos Juan , Medina-Miguelañez Mario , Puebla Laura , Román-Curto Concepción , Roncero-Riesco Mónica , Garcia Rosa , Ortiz Pablo L. , Rodriguez-Peralto Jose-Luis
Published: January 2021

The novel coronavirus disease (COVID-19) is a rapidly spreading pandemic, secondary to severe acute respiratory syndrome coronavirus 2. The severity and the little knowledge that we have of the disease have made us focus mostly on the respiratory symptoms. As we bend the curve, other findings reported in association with COVID-19 become of importance for specialists to recognize. We describe the spectrum of clinicopathologic lesions in the skin that can be the only symptom or the first manifestation of COVID-19 and demonstrate the origin of the virus. We collected 25 patients with skin lesions in this context. We recognized 5 types of cutaneous manifestations including acute acroischemic or chilblain-like lesions (11), purpura palpable (2), exanthemas (9), urticarial eruptions (1), and other lesions (2) that might appear with more unspecific pictures. Chilblain-like lesions were the most common form of presentation, which tend to appear as self-healing, erythematous-necrotic plaques mostly on the feet, in young patients with no systemic symptoms associated. Importantly, we visualized viral particles with electron microscopy in 5 of 13 cases analyzed. In this study, we seek to draw a picture of the spectrum of clinicopathologic lesions that may appear in the skin in the context of COVID-19. Although apparently skin lesions are not correlated with disease severity, it may help in some cases to recognize and control the spread of the infection sooner.
Gastric Inverted Polyps—Distinctive Subepithelial Lesions of the Stomach
Authors: Kim Ji-Ye , Ahn Soomin , Kim Kyoung-Mee , Chang Sun Hee , Kim Han Seong , Lee Jun Haeng , Kim Jae J. , Sohn Tae Sung , Kang Hye Ju , Joo Mee
Published: January 2021

Gastric inverted polyps (GIPs) are rare gastric polyps characterized by a submucosal inverted growth of mucosal components. Because of their rarity, they are not well characterized and are diagnostically challenging. We examined 12 cases of GIPs arising in 8 male and 4 female patients (mean age: 56 y). Most GIPs (11/12, 92%) occurred as a single, rounded subepithelial lesion in the body or fundus (mean size: 14.9 mm). Histologically, GIPs consisted of gastric-type glandular epithelium and smooth muscle component, growing in an endophytic manner; however, they displayed significant morphologic variations. We classified GIPs into 3 subtypes by the following features: communication with the mucosal surface, smooth muscle boundary, and tissue organization. The defining characteristics of type 1 were a mucosal communicating structure at the center and a well-defined smooth muscle boundary, resulting in a characteristic low-magnification morphology of a round vase. Type 2 had an organized glandular proliferation with smooth muscle boundary and no central communicating structure. Type 3 GIPs had no mucosal communicating structure or smooth muscle boundary; its key histologic feature was the lobular organization pattern produced by proliferations of cystic or hyperplastic glands and smooth muscle. All type 1 GIPs exhibited coexisting adenocarcinoma (3 cases) or stromal proliferation (3 cases). Three patients with type 2 GIP had separate adenocarcinoma. None of the type 3 GIPs had accompanying carcinoma. In conclusion, GIPs are a heterogenous group showing different morphology and clinical behavior. Notably, type 1 GIP could be considered a precancerous lesion with the potential to develop adenocarcinoma.
Monotypic Plasma Cell Proliferation of Uncertain Clinical Significance Mimicking Interstitial Cystitis
Authors: Vargas Ana Cristina , Burchett Ivan , Turner Jennifer , Delprado Warick , Brookwell Ross , Chalasani Venu , Gill Anthony J. , Maclean Fiona M.
Published: January 2021

We prospectively studied our institutional experience of bladder extranodal marginal zone (mucosa-associated lymphoid tissue [MALT]) lymphoma including bladder biopsies in which the possibility of MALT lymphoma was considered. We identified a subset of cases primary to the urinary bladder, presenting with prominent plasma cell infiltrates and symptoms mimicking bladder pain syndrome/interstitial cystitis. These proliferations were designated for this study as “monotypic plasma cell proliferation of uncertain clinical significance” (MPCP-US), as the features were insufficient for diagnosis of MALT lymphoma. We identified 33 patients, consisting of 22 cases of MPCP-US (6 of which were associated with amyloid deposition) and 11 cases of MALT lymphoma. MPCP-US was more prevalent in men (73%), a mass lesion was not identified at cystoscopy, and only 1 case had an accompanying urinary tract infection (4.5%). Histologically, MPCP-US presented as monotypic plasma cells arranged in a superficial band-like distribution in the lamina propria, predominantly kappa restricted (68%) and IgA+ or IgM+ (64% and 23%, respectively) and without a histologic mass of atypical B cells or plasma cells, not diagnostic for established MALT lymphoma or plasmacytoma. Secondary involvement of the bladder by other lymphoproliferative disorders was excluded and there was no evidence of progressive disease. MALT lymphomas are presented for comparison and our analysis demonstrated that MPCP-US represent a different clinicopathologic entity compared with classic MALT lymphoma. We present the first series of cases of MPCP-US. The recognition of this entity is fundamental to the development of management protocols to relieve intractable symptoms mimicking bladder pain syndrome/interstitial cystitis in these patients.
Androgen Receptor Immunohistochemical Expression in Undifferentiated/Dedifferentiated Endometrial Carcinoma
Authors: Cao Jin , Hacking Sean , Chavarria-Bernal Hector D. , Bhuiya Tawfiqul A. , Khutti Seema
Published: January 2021

Uterine undifferentiated (UC)/dedifferentiated (DEAC) carcinomas are rare malignant neoplasms. They tend to pursue an aggressive clinical course with an advanced stage at presentation. It has been found that androgen receptor (AR) might play a role as a prognostic and therapeutic marker in endometrial carcinoma. However, its expression in UC/DEAC has not been investigated. Herein, the aim of this study was to evaluate the expression of AR along with estrogen receptor (ER), progestin receptor (PR), and HER2 in UC/DEAC and also in other subtypes of high-grade endometrial carcinomas. Review of our pathology database over the period of 2011 to 2019 identified 16 UC/DEAC cases (N=16). We also randomly selected other high-grade endometrial carcinomas including FIGO 3 endometrioid carcinoma (N=9), serous carcinoma (N=8), clear cell carcinoma (N=12) and carcinosarcoma (N=10) for comparison. Immunohistochemical stains for AR, ER, PR, and HER2 were performed on all 55 cases. The protein expression was evaluated both quantitatively and qualitatively. In DEAC cases both the undifferentiated component and the well-differentiated component were recorded separately. Overall, variable degrees of AR reactivity (by Allred scoring method) was present in 63% of UC/DEACs(10/16), 67% of FIGO 3 endometrioid carcinomas (6/9), 88% of serous carcinomas (7/8), 80% of carcinosarcomas (8/10), and 9% of clear cell carcinoma (1/12). AR expression was most often seen with PR (70%) or ER (60%) staining in UC/DEACs. Thirteen cases of UC/DEACs were positive for at least 1 hormone receptor. HER2 was negative in all UC/DEACs. Almost all other high-grade carcinoma cases were negative for HER2 except 20% of carcinosarcoma (2/10) and 13% of serous carcinoma (1/8) which showed 3+ HER2. Loss of AR appears to be associated with worse clinicopathologic parameters in UC/DEAC. AR is highly expressed in UC/DEAC, and in the majority of FIGO 3 endometrioid carcinomas, serous carcinomas, and carcinosarcoma. These findings suggest a potential role for androgen inhibitors in the management of patients with these tumors.
Risk mapping for COVID-19 outbreaks in Australia using mobility data
Authors: Zachreson Cameron , Mitchell Lewis , Lydeamore Michael J. , Rebuli Nicolas , Tomko Martin , Geard Nicholas
Published: January 2021

COVID-19 is highly transmissible and containing outbreaks requires a rapid and effective response. Because infection may be spread by people who are pre-symptomatic or asymptomatic, substantial undetected transmission is likely to occur before clinical cases are diagnosed. Thus, when outbreaks occur there is a need to anticipate which populations and locations are at heightened risk of exposure. In this work, we evaluate the utility of aggregate human mobility data for estimating the geographical distribution of transmission risk. We present a simple procedure for producing spatial transmission risk assessments from near-real-time population mobility data. We validate our estimates against three well-documented COVID-19 outbreaks in Australia. Two of these were well-defined transmission clusters and one was a community transmission scenario. Our results indicate that mobility data can be a good predictor of geographical patterns of exposure risk from transmission centres, particularly in outbreaks involving workplaces or other environments associated with habitual travel patterns. For community transmission scenarios, our results demonstrate that mobility data add the most value to risk predictions when case counts are low and spatially clustered. Our method could assist health systems in the allocation of testing resources, and potentially guide the implementation of geographically targeted restrictions on movement and social interaction.
Long-distance transequatorial navigation using sequential measurements of magnetic inclination angle
Authors: Taylor Brian K. , Lohmann Kenneth J. , Havens Luke T. , Lohmann Catherine M. F. , Granger Jesse
Published: January 2021

Diverse taxa use Earth’s magnetic field in combination with other sensory modalities to accomplish navigation tasks ranging from local homing to long-distance migration across continents and ocean basins. Several animals have the ability to use the inclination or tilt of magnetic field lines as a component of a magnetic compass sense that can be used to maintain migratory headings. In addition, a few animals are able to distinguish among different inclination angles and, in effect, exploit inclination as a surrogate for latitude. Little is known, however, about the role that magnetic inclination plays in guiding long-distance migrations. In this paper, we use an agent-based modelling approach to investigate whether an artificial agent can successfully execute a series of transequatorial migrations by using sequential measurements of magnetic inclination. The agent was tested with multiple navigation strategies in both present-day and reversed magnetic fields. The findings (i) demonstrate that sequential inclination measurements can enable migrations between the northern and southern hemispheres, and (ii) demonstrate that an inclination-based strategy can tolerate a reversed magnetic field, which could be useful in the development of autonomous engineered systems that must be robust to magnetic field changes. The findings also appear to be consistent with the results of some animal navigation experiments, although whether any animal exploits a strategy of using sequential measurements of inclination remains unknown.
AffordableCaenorhabditis eleganstracking system for classroom use
Authors: Leonard Nicholas , Vidal-Gadea Andrés G.
Published: January 2021

For decades the nematodeC. eleganshas served as an outstanding research organism owing to its unsurpassed experimental amenability. This advantage has also made this tiny worm an attractive vehicle for science instruction across higher learning institutions. However, the prohibitive cost associated with the automated behavioral assessment of these animals remains an obstacle preventing their full adoption in undergraduate and high school settings. To improve this situation, we developed an inexpensive worm tracking system for use by high school interns and undergraduate students. Over the past two years this tracker has been successfully used by undergraduate students in our introductory Cell and Molecular lab (BSC220) at Illinois State University. Here we describe and demonstrate the use of our inexpensive worm tracking system.
Optimizing vaccine allocation for COVID-19 vaccines: potential role of single-dose vaccination
Authors: Matrajt Laura , Eaton Julia , Leung Tiffany , Dimitrov Dobromir , Schiffer Joshua T. , Swan David A. , Janes Holly
Published: January 2021

Most COVID-19 vaccines require two doses, however with limited vaccine supply, policymakers are considering single-dose vaccination as an alternative strategy. Using a mathematical model combined with optimization algorithms, we determined optimal allocation strategies with one and two doses of vaccine under various degrees of viral transmission. Under low transmission, we show that the optimal allocation of vaccine vitally depends on the single-dose efficacy (SDE). With high SDE, single-dose vaccination is optimal, preventing up to 22% more deaths than a strategy prioritizing two-dose vaccination for older adults. With low or moderate SDE, mixed vaccination campaigns with complete coverage of older adults are optimal. However, with modest or high transmission, vaccinating older adults first with two doses is best, preventing up to 41% more deaths than a singledose vaccination given across all adult populations. Our work suggests that it is imperative to determine the efficacy and durability of single-dose vaccines, as mixed or single-dose vaccination campaigns may have the potential to contain the pandemic much more quickly.