bluuebunny/crossref_metadata_2025 huggingface dataset:
A Self-Training-Based System for Die Defect Classification
Authors: Wu Ping-Hung , Lin Siou-Zih , Chang Yuan-Teng , Lai Yu-Wei , Chen Ssu-Han
Published: August 2024

With increasing wafer sizes and diversifying die patterns, automated optical inspection (AOI) is progressively replacing traditional visual inspection (VI) for wafer defect detection. Yet, the defect classification efficacy of current AOI systems in our case company is not optimal. This limitation is due to the algorithms’ reliance on expertly designed features, reducing adaptability across various product models. Additionally, the limited time available for operators to annotate defect samples restricts learning potential. Our study introduces a novel hybrid self-training algorithm, leveraging semi-supervised learning that integrates pseudo-labeling, noisy student, curriculum labeling, and the Taguchi method. This approach enables classifiers to autonomously integrate information from unlabeled data, bypassing the need for feature extraction, even with scarcely labeled data. Our experiments on a small-scale set show that with 25% and 50% labeled data, the method achieves over 92% accuracy. Remarkably, with only 10% labeled data, our hybrid method surpasses the supervised DenseNet classifier by over 20%, achieving more than 82% accuracy. On a large-scale set, the hybrid method consistently outperforms other approaches, achieving up to 88.75%, 86.31%, and 83.61% accuracy with 50%, 25%, and 10% labeled data. Further experiments confirm our method’s consistent superiority, highlighting its potential for high classification accuracy in limited-data scenarios.
Fuzzy Logic Prediction of Hypertensive Disorders in Pregnancy Using the Takagi–Sugeno and C-Means Algorithms
Authors: Campero-Jurado Israel , Robles-Camarillo Daniel , Ruiz-Vanoye Jorge A. , Xicoténcatl-Pérez Juan M. , Díaz-Parra Ocotlán , Salgado-Ramírez Julio-César , Marroquín-Gutiérrez Francisco , Ramos-Fernández Julio Cesar
Published: August 2024

Hypertensive disorders in pregnancy, which include preeclampsia, eclampsia, and chronic hypertension, complicate approximately 10% of all pregnancies in the world, constituting one of the most serious causes of mortality and morbidity in gestation. To help predict the occurrence of hypertensive disorders, a study based on algorithms that help model this health problem using mathematical tools is proposed. This study proposes a fuzzy c-means (FCM) model based on the Takagi–Sugeno (T-S) type of fuzzy rule to predict hypertensive disorders in pregnancy. To test different modeling methodologies, cross-validation comparisons were made between random forest, decision tree, support vector machine, and T-S and FCM methods, which achieved 80.00%, 66.25%, 70.00%, and 90.00%, respectively. The evaluation consisted of calculating the true positive rate (TPR) over the true negative rate (TNR), with equal error rate (EER) curves achieving a percentage of 20%. The learning dataset consisted of a total of 371 pregnant women, of which 13.2% were diagnosed with a condition related to gestational hypertension. The dataset for this study was obtained from the Secretaría de Salud del Estado de Hidalgo (SSEH), México. A random sub-sampling technique was used to adjust the class distribution of the data set, and to eliminate the problem of unbalanced classes. The models were trained using a total of 98 samples. The modeling results indicate that the T-S and FCM method has a higher predictive ability than the other three models in this research.
A New Student Performance Prediction Method Based on Belief Rule Base with Automated Construction
Authors: Liu Mingyuan , He Wei , Zhou Guohui , Zhu Hailong
Published: August 2024

Student performance prediction (SPP) is a pivotal task in educational analytics, enabling proactive interventions and optimized resource allocation by educators. Traditional SPP models are often hindered by their complexity and lack of interpretability. This study introduces a novel SPP framework, the Belief Rule Base with automated construction (Auto–BRB), designed to address these issues. Firstly, reference values are derived through data mining techniques. The model employs an IF–THEN rule-based system integrated with evidential reasoning to ensure both transparency and interpretability. Secondly, parameter optimization is achieved using the Projected Covariance Matrix Adaptive Evolution Strategy (P–CMA–ES), significantly enhancing model accuracy. Moreover, the Akaike Information Criterion (AIC) is then applied to fine-tune the balance between model accuracy and complexity. Finally, case studies on SPP have shown that the Auto–BRB model has an advantage over traditional models in terms of accuracy, while maintaining good interpretability. Therefore, Auto–BRB has excellent application effects in educational data analysis.
Dynamic Optimization of a Supply Chain Operation Model with Multiple Products
Authors: Lopez-Landeros Carlos E. , Valenzuela-Gonzalez Ricardo , Olivares-Benitez Elias
Published: August 2024

Determination of the optimal operational policy for an automotive supply chain is explored under a centralized management approach using dynamic programming. A deterministic optimal control model is proposed to meet multi-product demand over a period while minimizing a cost performance index for a five-echelon network. The production-inventory levels are the state variables and the raw material acquisition rates are the control variables to be decided in the problem. The novelties include parts mixing operations, assembly requirements, and a push–pull chain operation strategy. The continuous model is solved using Iterative Dynamic Programming, an algorithm with successful applications in chemical engineering problems. Its implementation here is the first in supply chain (SC) management models. The results demonstrate that the proposal is suitable to represent the dynamic behavior of the SC and provides useful information to outline a cooperative decision-making process. Managerial insights are derived to improve the resilience and efficiency of the chain.
The Effects of the Introduction of Volume-Based Liquidity Constraints in Portfolio Optimization with Alternative Investments
Authors: Barro Diana , Basso Antonella , Funari Stefania , Visentin Guglielmo Alessandro
Published: August 2024

Recently, liquidity issues in financial markets and portfolio asset management have attracted much attention among investors and scholars, fuelling a stream of research devoted to exploring the role of liquidity in investment decisions. In this paper, we aim to investigate the effects of introducing liquidity in portfolio optimization problems. For this purpose, first we consider three volume-based liquidity measures proposed in the literature and we build a new one particularly suited to portfolio optimization. Secondly, we formulate an extended version of the Markowitz portfolio selection problem, named mean–variance–liquidity, wherein the goal is to minimize the portfolio variance subject to the usual constraint on the expected portfolio return and an additional constraint on the portfolio liquidity. Thirdly, we consider a sensitivity analysis, with the aim to assess the trade-offs between liquidity and return, on the one hand, and between liquidity and risk, on the other hand. In the second part of the paper, the portfolio optimization framework is applied to a dataset of US ETFs comprising both standard and alternative, often illiquid, investments. The analysis is carried out with all the liquidity measures considered, allowing us to shed light on the relationships among risk, return and liquidity. Finally, we study the effects of the introduction of a Bitcoin ETF, as an asset with an extremely high expected return and risk.
A Sustainable Inventory Model with Advertisement Effort for Imperfect Quality Items under Learning in Fuzzy Monsoon Demand
Authors: Alamri Osama Abdulaziz , Lamba Navneet Kumar , Jayaswal Mahesh Kumar , Mittal Mandeep
Published: August 2024

In this paper, we proposed a sustainable inventory model with a learning effect for imperfect quality items under different kinds of fuzzy environments like crisp, general fuzzy, cloudy fuzzy, and monsoon fuzzy. We divided the mathematical model into three parts under the learning effect according to the real-time fuzzy components (crisp, cloudy, and monsoon environments) of the demand rate of the items. We minimized the total inventory cost with respect to cycle length in each environment under the proposed assumptions. The non-linear optimization technique is applied for the algorithm and the solution method to find the decision variable. Finally, we compared the total inventory cost under different fuzzy environments and our finding is that the fuzzy monsoon environment is a more effective fuzzy environment than crisp and cloudy fuzzy environments. We have presented a numerical example for the validation of the proposed model and have shown the impact of the inventory input parameters on the cycle length and total inventory fuzzy cost. The managerial insights and future scope of this proposed study have been shown in the sensitivity analysis and conclusion. The limitations, application, future extension and scope, and social implementation have been shown in this research study.
Factors Associated with Quality of Life among People with Atrial Fibrillation: Jordan Atrial Fibrillation Registry Study
Authors: Al-Bashaireh Ahmad M. , Alkouri Osama , Alharbi Abdulhafith , Khader Yousef , Hammoudeh Ayman , Aljawarneh Yousef , Alotaibi Nader E. , Qaladi Omar , Ababneh Anas , Schultz Tim
Published: August 2024

Background and Objectives: Atrial fibrillation (AF) is a common arrhythmia that may adversely affect the quality of life (QoL). Several factors could be associated with the QoL among patients with AF; however, evidence regarding these factors is still limited and controversial. Therefore, this study aimed to identify the level of QoL and its associated factors among Jordanian patients with AF. Subjects and methods: A case study design was implemented. A sample of 620 participants were recruited from 28 outpatient clinics registered in the Jordan atrial fibrillation registry AF (JoFIB). Data on QoL were gathered through the self-reported Atrial Fibrillation Effect on Quality-of-life tool (AFEQT). A QoL questionnaire was validated in this population before starting this study. The cardiac nurse then provided the research assistant with data relating to patients’ characteristics and associated comorbidities. Results: The overall AFEQT scores were positively skewed (median 21.3, IQR: 14.4–31.9). This pattern was reflected for the AFEQT sub-scales ‘Symptoms’ (20.8, 8.3–33.3), ‘Daily activities’ (16.7, 10.4–27.1), and ‘Treatment concerns’ (27.8, 19.4–41.7), whereas ‘Treatment satisfaction’ was negatively skewed (91.7, 83.3–91.7). Patients in the higher quartiles, indicating a better QoL, tended to be younger and were less likely to experience dyslipidemia, stroke, pulmonary hypertension, or other comorbidities. Over 90% of patients were currently experiencing AF, and patients with a better QoL tended to be less likely to be currently experiencing AF and more likely to have had their latest episode of AF more than a month ago (compared to less than a month ago). Age, BMI, dyslipidemia, heart failure, COPD, CAD, history of ablation, and the use of anticoagulants were significantly associated with the overall AFEQT score (R2 = 0.278). Conclusions: This study demonstrates that AF Jordanian patients had low levels of QoL. Patients in higher quartiles for the overall AFEQT score were younger, with fewer disease comorbidities and less experience of current AF episodes. Several modifiable and non-modifiable factors were associated with QoL in AF patients. Age, BMI, dyslipidemia, heart failure, COPD, CAD, history of ablation, and the use of oral anticoagulants were significantly associated with the overall AFEQT score. Healthcare providers should target these factors as indicators or interventions for which QoL is continuously monitored.
Investigation of Irradiation Hardening and Effectiveness of Post-Irradiation Annealing on the Recovery of Tensile Properties of VVER-1000 Realistic Welds Irradiated in the LYRA-10 Experiment
Authors: Laot Mathilde , Marques Pereira Viviam , Bakker Theo , d’Agata Elio , Martin Oliver , Kolluri Murthy
Published: August 2024

Assessing the embrittlement and hardening of reactor pressure vessel steels is critical for the extension of the service lifetime of nuclear power plants. This paper summarises the tensile test results on the irradiation behaviour of realistic VVER-1000 welds from the STRUMAT-LTO project. The welds were irradiated at the HFR (Petten, the Netherlands) to a fluence of up to 1.087 × 1020 n·cm−2, and their irradiation hardening was studied by means of tensile testing. The four grades, with different Mn and Ni contents, show different hardening behaviours. The highest degree of irradiation hardening is observed for the weld that has the highest combined Ni + Mn content. The results show that there is a synergetic effect of Mn and Ni on the irradiation hardening behaviour of the VVER-1000 welds. Besides irradiation hardening, the effectiveness of post-irradiation annealing treatments on the recovery of the tensile properties is studied in the present work. Post-irradiation annealing treatments conducted at 418 °C and at 475 °C proved to be effective for three of the four investigated welds. For the realistic weld with the highest combined Ni + Mn, only the annealing at 475 °C led to the complete recovery of the tensile properties.
Joint Quality Assessment of Ultrasonic Metal Welded Parts by Fracture Surface Evaluation
Authors: Müller Florian W. , Zhao Haolin , Schiebahn Alexander , Reisgen Uwe
Published: August 2024

In ultrasonic metal welding, low specific resistances and large joining surface cross-sections require the use of mechanical testing to quantify the joint quality. In this study, different quality features of ultrasonically welded joints made of pure copper sheet are investigated during the successive phases of joint formation. Two test series with different workpiece geometries are examined. It is shown that mechanical quality features such as shear and peel forces behave differently over the formation of the joint and are not transferable. As an alternative to these, laser scanning microscopy is used to record images of the fracture surface that describe the growth of the joint area during formation. The study finds that shear tensile force growth and joint area growth are non-linear and comparable, with optimized welds achieving joint areas of 30 mm2 out of 64 mm2 and 6 mm2 out of 16 mm2. Although overall quality increases with increasing welding time, the material strength in the joint zone decreases. Depending on the original rolling condition, between 43% and 59% of the original material strength can be identified as the joint strength. The automatic analysis of fracture images is a suitable alternative to mechanical testing for similar joints.
Characterizing Growth-Retarded Japanese Eels (Anguilla japonica): Insights into Metabolic and Appetite Regulation
Authors: Zeng Xiangbiao , Liu Jingwei , Chen Yiwen , Han Huan , Liu Yanhe , Xie Bin , Jiang Tianwei , Wong Chris Kong-Chu , Li Kang , Liu Liping
Published: August 2024

During field surveys and culture procedures, large growth disparities in Anguilla japonica have been observed. However, the potential causes are unknown. This study explored differences in digestive ability, metabolic levels, and transcriptomic profiles of appetite-related genes between growth-retarded eel (GRE) and normal-growing eel (NGE) under the same rearing conditions. The results showed that growth hormone (gh) mRNA expression in GREs was considerably lower than NGEs. The levels of total protein (TP), total cholesterol (T-CHO), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), blood ammonia (BA), blood urea nitrogen (BUN), and alkaline phosphatase (ALP) in GREs were significantly lower than in NGEs. Conversely, levels of glucose (GLU), alanine aminotransferase (ALT), and aspartate transaminase (AST) were higher in GREs. The activities of SOD, CAT, and T-AOC levels were also significantly lower in GREs, as were the activities of glucose-related enzymes including hexokinase (HK), pyruvate kinase (PK), phosphoenolpyruvate carboxykinase (PEPCK), and glucose-6-phosphatase (G6PASE). Additionally, orexigenic genes (npy and ghrelin) were dramatically downregulated, whereas anorexigenic genes (crh and pyy) were significantly upregulated in GREs. These findings suggested that variances in growth hormone, metabolic activities, and appetite level could be associated with the different growth rates of A. japonica. The present research not only revealed the characteristics of the growth, metabolism, and appetite of GREs but also offered new perspectives into the substantial growth discrepancies in A. japonica, providing novel ideas for enhancing fish growth.
Deciphering Blood Flow Restriction Training to Aid Lipid Lowering in Obese College Students through Untargeted Metabolomics
Authors: Cui Xianyou , Anatolevna Sidorenko Tatiana , Wang Yu
Published: August 2024

(1) Objective: The aim of this study was to observe the lipid-lowering effects of blood flow restriction training (BFR) combined with moderate-intensity continuous training (MICT) in obese college students by observing lipid-lowering hormones and untargeted metabolomics. (2) Methods: In this study, 14 obese college students were convened into three groups—MICT, MICT+BFR, and high-intensity interval training (HIIT)—for a crossover experiment. Blood was drawn before and after exercise for the analysis of lipolytic agents and untargeted metabolomics. The study used a paired t-test and ANOVA for statistical analyses. (3) Results: The lipolytic agent results showed that MICT+BFR was superior to the other two groups in terms of two agents (p = 0.000 and p = 0.003), namely, GH and IL-6 (difference between before and after testing: 10,986.51 ± 5601.84 and 2.42 ± 2.49, respectively), and HIIT was superior to the other two groups in terms of one agent (p = 0.000), i.e., EPI (22.81 ± 16.12). No advantage was observed for MICT. The metabolomics results showed that, compared to MICT, MICT+BFR was associated with the upregulated expression of xanthine, succinate, lactate, N-lactoylphenylalanine, citrate, ureido acid, and myristic acid after exercise, with the possibility of the involvement of the citric acid cycle, alanine, aspartic acid, glutamate metabolism, butyric acid metabolism, and the histidylate metabolism pathway. (4) Conclusions: The superior lipid-lowering effect of MICT+BFR over MICT in a group of obese college students may be due to the stronger activation of GH and IL-6 agents, with the citric acid cycle and alanine, aspartate, and glutamate metabolic pathways being associated with this type of exercise.
Indirect Enhancement of ALD Thin-Film Properties Induced by the ECAP Modification of an As-Extruded Mg-Ca Alloy
Authors: Lin Pi-Chen , Li Jun-Yu , Chen Hou-Jen , Lin Kaifan , Chen Miin-Jang , Lin Kun-Ming , Lin Hsin-Chih
Published: August 2024

The purpose of this study is to investigate the indirect effects on the properties of ZrO2 films deposited by atomic layer deposition (ALD) when an Mg-Ca alloy is modified through equal-channel angular pressing (ECAP) following extrusion. The study aims to understand how the increase in CaO content in the native oxide layer of the Mg-Ca alloy influences the crystallinity and defect density of the ZrO2 film. Consequently, the corrosion protection performance of the ZrO2 film is enhanced by 1.2 to 1.5 times. A reduction in the anti-scratch property of the ZrO2 film was also observed, with a critical load reduction of 34 μN. This research provides a detailed analysis of the modifications induced by ECAP on the as-extruded Mg-Ca alloy and its subsequent impact on the properties of the ZrO2 film.
Recent Advances in Photoacoustic Imaging: Current Status and Future Perspectives
Authors: Liu Huibin , Teng Xiangyu , Yu Shuxuan , Yang Wenguang , Kong Tiantian , Liu Tangying
Published: August 2024

Photoacoustic imaging (PAI) is an emerging hybrid imaging modality that combines high-contrast optical imaging with high-spatial-resolution ultrasound imaging. PAI can provide a high spatial resolution and significant imaging depth by utilizing the distinctive spectroscopic characteristics of tissue, which gives it a wide variety of applications in biomedicine and preclinical research. In addition, it is non-ionizing and non-invasive, and photoacoustic (PA) signals are generated by a short-pulse laser under thermal expansion. In this study, we describe the basic principles of PAI, recent advances in research in human and animal tissues, and future perspectives.
Two CMOS Wilkinson Power Dividers Using High Slow-Wave and Low-Loss Transmission Lines
Authors: Pakasiri Chatrpol , Teng Wei-Sen , Wang Sen
Published: August 2024

This work presents two Wilkinson power dividers (WPDs) using multi-layer pseudo coplanar waveguide (PCPW) structures. The PCPW-based WPDs were designed, implemented, and verified in a standard 180 nm CMOS process. The proposed PCPW features high slow-wave and low-loss performances compared to other common transmission lines. The two WPDs are based on the same PCPW structure parameters in terms of line width, spacing, and used metal layers. One WPD was realized in a straight PCPW-based layout, and the other WPD was realized in a meandered PCPW-based layout. Both the two WPDs worked up to V-band frequencies, as expected, which also demonstrates that the PCPW guiding structure is less susceptible to the effects of meanderings on the propagation constant and characteristic impedance. The meandered design shows that the measured insertion losses were about 5.1 dB, and its return losses were better than 17.5 dB at 60 GHz. In addition, its isolation, amplitude imbalance, and phase imbalance were 18.5 dB, 0.03 dB, and 0.4°, respectively. The core area was merely 0.2 mm × 0.23 mm, or 1.8 × 10−3λo2.
Phenolic Compounds Synthesized by Trichoderma longibrachiatum Native to Semi-Arid Areas Show Antifungal Activity against Phytopathogenic Fungi of Horticultural Interest
Authors: Díaz-García Enis , Valenzuela-Quintanar Ana Isabel , Sánchez-Estrada Alberto , González-Mendoza Daniel , Tiznado-Hernández Martín Ernesto , Islas-Rubio Alma Rosa , Troncoso-Rojas Rosalba
Published: August 2024

Fungal diseases are a major threat to the horticultural industry and cause substantial postharvest losses. While secondary metabolites from Trichoderma sp. have been explored for their antifungal properties, limited information exists on the phenolic compounds produced by less studied species like Trichoderma longibrachiatum. In this study, phenolic compounds were extracted from a liquid culture of T. longibrachiatum using various solvents and methods (conventional and ultrasonic-assisted). Phenolic compounds were quantified by spectrophotometry and identified by high-performance liquid chromatography with diode array detection (HPLC-DAD). The antifungal activity against Alternaria alternata and Fusarium oxysporum was determined by mycelial growth inhibition assays, maximum growth rate (µmax) by the Gompertz equation, and spore germination tests. Although no significant differences (p ≥ 0.05) were found between the extraction methods, the type of solvent significantly influenced the phenolic content (p ≤ 0.05). Extraction with 70% ethanol showed the highest content of phenolic compounds and flavonoids. More than eight phenolic compounds were detected. Further, this is the first report of the phenolics ferulic, chlorogenic and p-coumaric acids identification in T. longibrachiatum, along with flavonoids such as epicatechin and quercetin, among others. The 70% ethanolic extracts notably inhibited the mycelial growth of A. alternata and F. oxysporum, reducing their maximum growth rate by 1.5 and 1.4 mm/h, respectively. Furthermore, p-coumaric and ferulic acids significantly inhibited spore germination of both pathogens, with a minimum inhibitory concentration (MIC) of 1.5 mg/mL and a minimum fungicidal concentration (MFC) of 2 mg/mL. These findings demonstrate the potential of T. longibrachiatum and its phenolic compounds as viable alternatives for biological control in horticulture and postharvest disease management.
Inhibition of the Growth of Escherichia coli and Staphylococcus aureus Microorganisms in Aesthetic Orthodontic Brackets through the In Situ Synthesis of Ag, TiO2 and Ag/TiO2 Nanoparticles
Authors: Sánchez Reyna Paola Ariselda , Olea Mejía Oscar Fernando , González-Pedroza María G. , Montiel-Bastida Norma M. , Rebollo-Plata Bernabe , Morales-Luckie Raúl A.
Published: August 2024

Plaque control is especially important during orthodontic treatment because areas of the teeth near brackets and wires are difficult to clean with a toothbrush, resulting in debris buildup of food or dental plaque, thus causing caries and periodontal disease. The objective of this study was to evaluate the antimicrobial properties of silver nanoparticles (AgNPs), titanium dioxide nanoparticles (TiO2NPs), and silver/titanium dioxide nanoparticles (Ag/TiO2NPs), synthesized on the surface of α-alumina ceramic brackets. The AgNPs and TiO2NPs were synthesized by a simple chemical method, and these were characterized by XRD, SEM, and XPS TEM; the antimicrobial activity was tested against Staphylococcus aureus and Escherichia coli by diffusion test. The results of this study demonstrated that by this simple chemical method, silver and titanium dioxide nanoparticles can be synthesized on the surface of α-alumina esthetic brackets, and these NPs possess good antimicrobial activity and the possibility of reducing dental caries, periodontal disease, and white spot generated during orthodontic treatment.
Trends in SARS-CoV-2 Cycle Threshold Values in Bosnia and Herzegovina—A Retrospective Study
Authors: Moro Almedina , Softić Adis , Travar Maja , Goletić Šejla , Omeragić Jasmin , Koro-Spahić Amira , Kapo Naida , Mrdjen Visnja , Terzić Ilma , Ostojic Maja , Cerkez Goran , Goletic Teufik
Published: August 2024

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which led to the COVID-19 pandemic, has significantly impacted global public health. The proper diagnosis of SARS-CoV-2 infection is essential for the effective control and management of the disease. This study investigated the SARS-CoV-2 infection using RT-qPCR tests from laboratories in Bosnia and Herzegovina. We performed a retrospective study of demographic data and Ct values from 170,828 RT-qPCR tests from April 2020 to April 2023, representing 9.3% of total national testing. Samples were collected from 83,413 individuals across different age groups. Of all tests, 33.4% were positive for SARS-CoV-2, with Ct values and positivity rates varying across demographics and epidemic waves. The distribution was skewed towards older age groups, although lower positivity rates were observed in younger age groups. Ct values, indicative of viral load, ranged from 12.5 to 38. Lower Ct values correlated with higher positive case numbers, while higher Ct values signaled outbreak resolution. Additionally, Ct values decreased during epidemic waves but increased with the dominance of certain variants. Ct value-distribution has changed over time, particularly after the introduction of SARS-CoV-2 variants of interest/concern. Established Ct value trends might, therefore, be used as an early indicator and additional tool for informed decisions by public health authorities in SARS-CoV-2 and future prospective pandemics. Moreover, they should not be overlooked in future epidemiological events.
A Bead Biofilm Reactor for High-Throughput Growth and Translational Applications
Authors: Gilmore Annika , Badham Marissa , Rudisin Winston , Ashton Nicholas , Williams Dustin
Published: August 2024

Bacteria in natural ecosystems such as soil, dirt, or debris preferentially reside in the biofilm phenotype. When a traumatic injury, such as an open fracture, occurs, these naturally dwelling biofilms and accompanying foreign material can contaminate the injury site. Given their high tolerance of systemic levels of antibiotics that may be administered prophylactically, biofilms may contribute to difficult-to-treat infections. In most animal models, planktonic bacteria are used as initial inocula to cause infection, and this might not accurately mimic clinically relevant contamination and infection scenarios. Further, few approaches and systems utilize the same biofilm and accompanying substrate throughout the experimental continuum. In this study, we designed a unique reactor to grow bacterial biofilms on up to 50 silica beads that modeled environmental wound contaminants. The data obtained indicated that the reactor system repeatably produced mature Staphylococcus aureus and Pseudomonas aeruginosa biofilms on the silica beads, with an average of 5.53 and 6.21 log10 colony-forming units per mm2, respectively. The bead substrates are easily manipulable for in vitro or in vivo applications, thus improving translatability. Taken together, the bead biofilm reactor presented herein may be a useful system for repeatably growing established biofilms on silica beads that could be used for susceptibility testing and as initial inocula in future animal models of trauma-related injuries.
Resistance and Co-Resistance of Metallo-Beta-Lactamase Genes in Diarrheal and Urinary-Tract Pathogens in Bangladesh
Authors: Shanta Ayasha Siddique , Islam Nahidul , Al Asad Mamun , Akter Kakoli , Habib Marnusa Binte , Hossain Md. Jubayer , Nahar Shamsun , Godman Brian , Islam Salequl
Published: August 2024

Carbapenems are the antibiotics of choice for treating multidrug-resistant bacterial infections. Metallo-β-lactamases (MBLs) are carbapenemases capable of hydrolyzing nearly all therapeutically available beta-lactam antibiotics. Consequently, this research assessed the distribution of two MBL genes and three β-lactamases and their associated phenotypic resistance in diarrheal and urinary-tract infections (UTIs) to guide future policies. Samples were collected through a cross-sectional study, and β-lactamase genes were detected via PCR. A total of 228 diarrheal bacteria were isolated from 240 samples. The most predominant pathogens were Escherichia coli (32%) and Klebsiella spp. (7%). Phenotypic resistance to amoxicillin-clavulanic acid, aztreonam, cefuroxime, cefixime, cefepime, imipenem, meropenem, gentamicin, netilmicin, and amikacin was 50.4%, 65.6%, 66.8%, 80.5%, 54.4%, 41.6%, 25.7%, 41.2%, 37.2%, and 42.9%, respectively. A total of 142 UTI pathogens were identified from 150 urine samples. Klebsiella spp. (39%) and Escherichia coli (24%) were the major pathogens isolated. Phenotypic resistance to amoxicillin-clavulanic acid, aztreonam, cefuroxime, cefixime, cefepime, imipenem, meropenem, gentamicin, netilmicin, and amikacin was 93.7%, 75.0%, 91.5%, 93.7%, 88.0%, 72.5%, 13.6%, 44.4%, 71.1%, and 43%, respectively. Twenty-four diarrheal isolates carried blaNDM-1 or blaVIM genes. The overall MBL gene prevalence was 10.5%. Thirty-six UTI pathogens carried either blaNDM-1 or blaVIM genes (25.4%). Seven isolates carried both blaNDM-1 and blaVIM genes. MBL genes were strongly associated with phenotypic carbapenem and other β-lactam antibiotic resistance. blaOXA imparted significantly higher phenotypic resistance to β-lactam antibiotics. Active surveillance and stewardship programs are urgently needed to reduce carbapenem resistance in Bangladesh.
Identification, Characterization, and Antibacterial Evaluation of Five Endophytic Fungi from Psychotria poeppigiana Müll. Arg., an Amazon Plant
Authors: Mendieta-Brito Sonia , Sayed Mahmoud , Son Eunjung , Kim Dong-Seon , Dávila Marcelo , Pyo Sang-Hyun
Published: August 2024

Endophytic fungi, residing within plants without causing disease, are known for their ability to produce bioactive metabolites with diverse properties such as antibacterial, antioxidant, and antifungal activities, while also influencing plant defense mechanisms. In this study, five novel endophytic fungi species were isolated from the leaves of Psychotria poeppigiana Müll. Arg., a plant from the Rubiaceae family, collected in the tropical Amazon region of Bolivia. The endophytic fungi were identified as a Neopestalotiopsis sp., three Penicillium sp., and an Aspergillus sp. through 18S ribosomal RNA sequencing and NCBI-BLAST analysis. Chemical profiling revealed that their extracts obtained by ethyl acetate contained terpenes, flavonoids, and phenolic compounds. In a bioautography study, the terpenes showed high antimicrobial activity against Escherichia coli. Notably, extracts from the three Penicillium species exhibited potent antibacterial activity, with minimum inhibitory concentration (MIC) values ranging from 62.5 to 2000 µg/mL against all three pathogens: Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis (both Gram-positive and Gram-negative bacteria). These findings highlight the potential of these endophytic fungi, especially Penicillium species as valuable sources of secondary metabolites with significant antibacterial activities, suggesting promising applications in medicine, pharmaceuticals, agriculture, and environmental technologies.
Use of Phosphorus-Solubilizing Microorganisms as a Biotechnological Alternative: A Review
Authors: Ramos Cabrera Efrén Venancio , Delgado Espinosa Zuly Yuliana , Solis Pino Andrés Felipe
Published: August 2024

Microorganisms with the ability to dissolve phosphorus have the potential to release this essential nutrient into the soil through natural solubilization processes, which allows for boosting plant growth and development. While literature reviews acknowledge their potential, unexplored territories concerning accessibility, application, and effective integration into sustainable agriculture necessitate further research. This manuscript employed distinct methodologies to execute a bibliometric analysis and a literature review. The combined application of both methodologies enables a holistic understanding of the domain landscape and its innovative facets. For the bibliometric analysis, the propositions of Donthu and Jia were utilized, supplemented by tools, such as Bibliometrix. The literature review adhered to a systematic methodology predicated on Petersen’s guidelines to represent the domain accurately, pinpointing trends and gaps that could steer future, more detailed research. This investigation uncovers an escalating interest in studying these microorganisms since the 2000s, emphasizing their significance in sustainable agriculture and the context of phosphorus scarcity. It was also discerned that India and China, nations with notable agricultural sectors and a high demand for phosphorus fertilizers, spearheaded research output on this subject. This signifies their substantial contribution to the progression of this scientific field. Furthermore, according to the research consulted, phosphorus-solubilizing microorganisms play a pivotal role in the symbiotic interaction of soil with plant roots and represent an efficacious strategy to counteract the low availability of phosphorus in the soil and sustainably enhance agricultural systems. Finally, this review contributes to the relevant domain by examining existing empirical evidence with special emphasis on sustainable agriculture, improved understanding of phosphorus solubilization mechanisms, and recognition of various microbial entities.
Multidisciplinary Effort Leading to Effective Tuberculosis Community Outbreak Containment in Israel
Authors: Fuchs Inbal , Losev Yelena , Mor Zohar , Rubinstein Mor , Polyakov Marina , Wagner Tali , Gobay Tamar , Bayene Ester , Mula Gila , Kaidar-Shwartz Hasia , Dveyrin Zeev , Rorman Efrat , Kaliner Ehud , Perl Sivan Haia
Published: August 2024

Tuberculosis (TB) is the second-most prevalent cause of mortality resulting from infectious diseases worldwide. It is caused by bacteria belonging to the Mycobacterium tuberculosis complex (MTBC). In Israel, TB incidence is low, acknowledged by the WHO as being in a pre-elimination phase. Most cases occur among immigrants from high TB incidence regions like the Horn of Africa and the former Soviet Union (FSU), with occasional outbreaks. The outbreak described in this report occurred between 2018 and 2024, increasing the incidence rate of TB in the region. Control of this outbreak posed challenges due to factors including a diverse population (including Ethiopian immigrants, Israeli-born citizens, and immigrants from other countries), economic and social barriers, and hesitancy to disclose information. The unique multidisciplinary team formed to address these challenges, involving the local TB clinic, district health ministry, health maintenance organization (HMO) infectious disease consultant, neighborhood clinic, and National Mycobacterium Reference Laboratory (NMRL), achieved effective treatment and containment. Whole genome sequencing (WGS) proved pivotal in unraveling patient connections during the outbreak. It pinpointed those patients overlooked in initial field investigations, established connections between patients across different health departments, and uncovered the existence of two distinct clusters with separate transmission chains within the same neighborhood. This study underscores collaborative efforts across sectors that successfully contained a challenging outbreak.
Geochronology, Petrogenesis, and Metallogenic Implications of Quartz Monzonite Porphyry in the Shanlixu Copper–Gold Deposit in the Lujiang–Chuzhou Area, Middle–Lower Yangtze River Valley Metallogenic Belt, China
Authors: Cai Yang , Tang Cheng , Ma Tao , Shi Ke , Li Ziteng , Fan Siwen
Published: August 2024

The Lujiang–Chuzhou Metallogenic Area is an important component of the Middle–Lower Yangtze River Valley Metallogenic Belt. Despite being an important copper–gold deposit in this area, the Shanlixu skarn Cu-Au deposit has not yet been systematically studied. According to LA-ICP-MS zircon U-Pb dating, the quartz monzonite porphyry from the Shanlixu deposit is aged 137.5 ± 1.7 Ma: while it differs from the timing of the magmatism and related mineralization in the Lujiang–Chuzhou Area, it is consistent with magmatic activity elsewhere in the Middle–Lower Yangtze River Valley Metallogenic Belt. The Ce4+/Ce3+ values of zircon in the quartz monzonite porphyry vary from 204.5 to 886.5, indicating that the intrusion might have formed in an environment with high oxygen fugacity. Additionally, the quartz monzonite porphyry exhibits high contents of Al2O3, Sr, Ba, and Mg# (Mg# = Mg2+/(Mg2+ + Fe2+)) and low ratios of Y, Nb, Ta, and K2O/Na2O, showing geochemical characteristics similar to those of adakitic rocks. Based on these characteristics, it is suggested that the intrusion might have been derived from the partial melting of subducted oceanic crust under a continental arc margin setting. Furthermore, it is strongly indicated that the quartz monzonite porphyry from the Shanlixu deposit, in the Lujiang–Chuzhou Area, is closely related to Cu-Au mineralization, as suggested by the age of the intrusion, which is approximately 137 Ma. These findings provide a new direction for research and exploration in this region.
The Impact of Drying Methods on the Quality of Blanched Yellow Mealworm (Tenebrio molitor L.) Larvae
Authors: Bogusz Radosław , Bryś Joanna , Onopiuk Anna , Pobiega Katarzyna , Tomczak Aneta , Kowalczewski Przemysław Łukasz , Rybak Katarzyna , Nowacka Małgorzata
Published: August 2024

The growing world population necessitates the implementation of appropriate processing technologies for edible insects. The objective of this study was to examine the impact of distinct drying techniques, including convective drying at 70 °C (70CD) and 90 °C (90CD) and freeze-drying (FD), on the drying kinetics, physical characteristics (water activity, color), chemical characteristics (chemical composition, amino acid profile, oil properties, total polyphenol content and antioxidant activity, mineral composition, FTIR), and presence of hazards (allergens, microorganisms) of blanched yellow mealworm larvae. The freeze-drying process results in greater lightness and reduced moisture content and water activity. The study demonstrated that the freeze-dried insects exhibited lower contents of protein and essential amino acids as compared to the convective-dried insects. The lowest content of total polyphenols was found in the freeze-dried yellow mealworm larvae; however, the highest antioxidant activity was determined for those insects. Although the oil isolated from the freeze-dried insects exhibited the lowest acid and peroxide values, it proved to have the lowest PUFA content and oxidative stability. All the samples met the microbiological criteria for dried insects. The results of the study demonstrate that a high temperature during the CD method does not result in the anticipated undesirable changes. It appears that freeze-drying is not the optimal method for preserving the nutritional value of insects, particularly with regard to the quality of protein and oil.
Carbonized Ganoderma Lucidum/V2O3 Composites as a Superior Cathode for High-Performance Aqueous Zinc-Ion Batteries
Authors: Zeng Guilin , Li Zhengda , Jiang Shaohua , Zhou Wei
Published: August 2024

In response to the suboptimal electrochemical performance of low-valence vanadium oxides, Ganoderma lucidum biomass-derived carbon@V2O3 (V2O3@CGL) composites were prepared by evaporative self-assembly technology and high-temperature calcination. In the prepared composites, V2O3 effectively encapsulates CGL, serving as a support for V2O3 and enhancing electrical conductivity and structural stability. This results in improved overall performance for the composites. They revealed satisfactory electrochemical properties when assembled in aqueous zinc-ion batteries (AZIBs). The preliminary discharge specific capacity of the V2O3@CGL-2 (VOCG-2) composite electrode reached 407.87 mAh g−1 at 0.05 A g−1. After 1000 cycles, the capacity retention is 93.69% at 3 A g−1. This research underscores the feasibility of employing V2O3 and abundantly available biomass for high-performance AZIB cathodes.
Comprehensive Review of Synthesis, Optical Properties and Applications of Heteroarylphosphonates and Their Derivatives
Authors: Owsianik Krzysztof , Romaniuk Adrian , Turek Marika , Bałczewski Piotr
Published: August 2024

This review focuses on optical properties of compounds in which at least one phosphonate group is directly attached to a heteroaromatic ring. Additionally, the synthesis and other applications of these compounds are addressed in this work. The influence of the phosphonate substituent on the properties of the described compounds is discussed and compared with other non-phosphorus substituents, with particular attention given to photophysical properties, such as UV-Vis absorption and emission, fluorescence quantum yield and fluorescence lifetime. Considering the presence of heteroatom, the collected material was divided into two parts, and a review of the literature of the last thirty years on heteroaryl phosphonates containing sulfur and nitrogen atoms in the aromatic ring was conducted.
Utilization of Lead Nitrate to Enhance the Impact of Hydroxamic Acids on the Hydrophobic Aggregation and Flotation Behavior of Cassiterite
Authors: Jin Saizhen , Liu Xiaobo , Feng Yun , Chen Yanfei , Wang Mengtao , Xiao Qingfei
Published: August 2024

Lead nitrate (LN) is frequently employed as an activator in the flotation of cassiterite using hydroxamic acids as the collectors. This study investigated the effect of LN on the hydrophobic aggregation of cassiterite when benzohydroxamic acid (BHA), hexyl hydroxamate (HHA), and octyl hydroxamate (OHA) were used as the collectors through micro-flotation, focused beam reflectance measurement (FBRM) and a particle video microscope (PVM), zeta potential, and the extended DLVO theory. Micro-flotation tests confirmed that LN activated the flotation of cassiterite using the hydroxamic acids as collectors. Focused beam reflectance measurement (FBRM) and a particle video microscope (PVM) were used to capture in situ data on the changes in size distribution and morphology of cassiterite aggregates during stirring. The FBRM and PVM image results indicated that the addition of LN could promote the formation of hydrophobic aggregates of fine cassiterite, when BHA or HHA was used as the collector, and reduce the dosage of OHA needed to induce the formation of hydrophobic aggregates of cassiterite. The extended DLVO theory interaction energies indicated that the presence of LN could decrease the electrostatic interaction energies (Vedl) and increase the hydrophobic interaction energies (Vhy) between cassiterite particles, resulting in the disappearance of the high energy barriers that existed between the particles in the absence of LN. Thus, cassiterite particles could aggregate in the presence of LN when BHA, HHA, or a low concentration of OHA was used as the collector.
Corrosion and Interfacial Contact Resistance of NiTi Alloy as a Promising Bipolar Plate for PEMFC
Authors: Li Yingping , Wang Xiaofen , Li Yuanyuan , He Zhuo , Zhang Guohong , Wang Zhen , Wang Shaohua , Hu Fei , Zhou Qiongyu
Published: August 2024

Titanium (Ti) is generally considered as an ideal bipolar plate (BPP) material because of its excellent corrosion resistance, good machinability and lightweight nature. However, the easy-passivation property, which leads to increased interfacial contact resistance (ICR) and subsequently decreased cell performance, limits its large-scale commercial application in proton exchange membrane fuel cells (PEMFCs). In this paper, we proposed a NiTi alloy prepared by suction casting as a promising bipolar plate for PEMFCs. This NiTi alloy exhibits significantly decreased ICR values (16.8 mΩ cm2 at 1.4 MPa) compared with pure Ti (88.6 mΩ cm2 at 1.4 MPa), along with enhanced corrosion resistance compared with pure nickel (Ni). The superior corrosion resistance of NiTi alloy is accredited to the nobler open circuit potential and corrosion potential, coupled with low corrosion current densities and passive current densities. The improved ICR can be interpreted by the existence of high-proportioned metallic Ni in the passive film, which contributes to the reduced capacitance characteristic of the passive film (compared with Ti) and enhances charge conduction. This work provides a feasible option to ameliorate BPP material that may have desirable corrosion resistance and ICR.
Gold Nanoparticle Mesoporous Carbon Composite as Catalyst for Hydrogen Evolution Reaction
Authors: Biehler Erik , Quach Qui , Abdel-Fattah Tarek M.
Published: August 2024

Increased environmental pollution and the shortage of the current fossil fuel energy supply has increased the demand for eco-friendly energy sources. Hydrogen energy has become a potential solution due to its availability and green combustion byproduct. Hydrogen feedstock materials like sodium borohydride (NaBH4) are promising sources of hydrogen; however, the rate at which the hydrogen is released during its reaction with water is slow and requires a stable catalyst. In this study, gold nanoparticles were deposited onto mesoporous carbon to form a nano-composite catalyst (AuNP-MCM), which was then characterized via transmission electron microscopy (TEM), powder X-ray diffraction (P-XRD), and scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM/EDS). The composite’s catalytic ability in a hydrogen evolution reaction was tested under varying conditions, including NaBH4 concentration, pH, and temperature, and it showed an activation of energy of 30.0 kJ mol−1. It was determined that the optimal reaction conditions include high NaBH4 concentrations, lower pH, and higher temperatures. This catalyst, with its stability and competitively low activation energy, makes it a promising material for hydrogen generation.