bluuebunny/crossref_metadata_2025 huggingface dataset:
Siloxane-Starch-Based Hydrophobic Coating for Multiple Recyclable Cellulosic Materials
Authors: Ganicz Tomasz , Rozga-Wijas Krystyna
Published: September 2021

The results of the application of a new hydrophobization agent based on a triethoxymethylsilane and standard starch aqueous mixture for mass-produced cellulosic materials—printing paper, paperboard, and sack paper—have been evaluated to examine whether such a mixture can be used in industrial practice. The application of this agent on laboratory sheets prepared in a repetitive recycling process was performed to investigate its influence on the formation and properties of the products, as well as the contamination of circulating water. Measurements of the water contact angle, Cobb tests, and water penetration dynamics (PDA) were performed to test the barrier properties of the resulting materials. The effects of the applied coatings and recycling process on the paper’s tensile strength, tear index, roughness, air permeance, and ISO brightness were studied. Studies have proven that this formulation imparts relatively high surface hydrophobicity to all materials tested (contact angles above 100°) and a significant improvement in barrier properties while maintaining good mechanical and optical performance. The agent also does not interfere with the pulping and re-forming processes during recycling and increases circulation water contamination to an acceptable degree. Attenuated total reflectance Fourier-transform infrared (FT-IR) spectra of the paper samples revealed the presence of a polysiloxane network on the surface.
A Robust and Highly Precise Alternative against the Proliferation of Intestinal Carcinoma and Human Hepatocellular Carcinoma Cells Based on Lanthanum Strontium Manganite Nanoparticles
Authors: Turky Ali Omar , Abdelmoaz Miral A. , Hessien Mahmoud M. , Hassan Ali M. , Bechelany Mikhael , Ewais Emad M. , Rashad Mohamed M.
Published: September 2021

In this report, lanthanum strontium manganite at different Sr2+ ion concentrations, as well as Gd3+ or Sm3+ ion substituted La0.5−YMYSr0.5MnO3 (M = Gd and Sm, y = 0.2), have been purposefully tailored using a sol gel auto-combustion approach. XRD profiles confirmed the formation of a monoclinic perovskite phase. FE-SEM analysis displayed a spherical-like structure of the La0.8Sr0.2MnO3 and La0.3Gd0.2Sr0.2MnO3 samples. The particle size of the LSM samples was found to decrease with increased Sr2+ ion concentration. For the first time, different LSM concentrations were inspected for their cytotoxic activity against CACO-2 (intestinal carcinoma cells) and HepG-2 (human hepatocellular carcinoma cells). The cell viability for CACO-2 and HepG-2 was assayed and seen to decrease depending on the Sr2+ ion concentration. Half maximal inhibitory concentration IC50 of CACO-2 cell and HepG-2 cell inhibition was connected with Sr2+ ion ratio. Low IC50 was noticable at low Sr2+ ion content. Such results were correlated to the particle size and the morphology. Indeed, the IC50 of CACO-2 cell inhibition by LSM at a strontium content of 0.2 was 5.63 ± 0.42 µg/mL, and the value increased with increased Sr2+ ion concentration by up to 0.8 to be = 25 ± 2.7 µg/mL. Meanwhile, the IC50 of HepG-2 cell inhibition by LSM at a strontium content of 0.2 was 6.73 ± 0.4 µg/mL, and the value increased with increased Sr2+ ion concentration by up to 0.8 to be 31± 3.1 µg/mL. All LSM samples at different conditions were tested as antimicrobial agents towards fungi, Gram positive bacteria, and Gram negative bacteria. For instance, all LSM samples were found to be active towards Gram negative bacteria Escherichia coli, whereas some samples have presumed antimicrobial effect towards Gram negative bacteria Proteus vulgaris. Such results confirmed that LSM samples possessed cytotoxicity against CACO-2 and HepG-2 cells, and they could be considered to play a substantial role in pharmaceutical and therapeutic applications.
Hyaluronic-Acid-Based Organic-Inorganic Composites for Biomedical Applications
Authors: Sikkema Rebecca , Keohan Blanca , Zhitomirsky Igor
Published: September 2021

Applications of natural hyaluronic acid (HYH) for the fabrication of organic-inorganic composites for biomedical applications are described. Such composites combine unique functional properties of HYH with functional properties of hydroxyapatite, various bioceramics, bioglass, biocements, metal nanoparticles, and quantum dots. Functional properties of advanced composite gels, scaffold materials, cements, particles, films, and coatings are described. Benefiting from the synergy of properties of HYH and inorganic components, advanced composites provide a platform for the development of new drug delivery materials. Many advanced properties of composites are attributed to the ability of HYH to promote biomineralization. Properties of HYH are a key factor for the development of colloidal and electrochemical methods for the fabrication of films and protective coatings for surface modification of biomedical implants and the development of advanced biosensors. Overcoming limitations of traditional materials, HYH is used as a biocompatible capping, dispersing, and structure-directing agent for the synthesis of functional inorganic materials and composites. Gel-forming properties of HYH enable a facile and straightforward approach to the fabrication of antimicrobial materials in different forms. Of particular interest are applications of HYH for the fabrication of biosensors. This review summarizes manufacturing strategies and mechanisms and outlines future trends in the development of functional biocomposites.
Corrosion Behavior of Zn, Fe and Fe-Zn Powder Materials Prepared via Uniaxial Compression
Authors: Gorejová Radka , Šišoláková Ivana , Cipa Pavol , Džunda Róbert , Sopčák Tibor , Oriňak Andrej , Oriňaková Renáta
Published: September 2021

Powder metallurgy is one of the most prevalent ways for metallic degradable materials preparation. Knowledge of the properties of initial powders used during this procedure is therefore of great importance. Two different metals, iron and zinc, were selected and studied in this paper due to their promising properties in the field of biodegradable implants. Raw powders were studied using scanning electron microscopy (SEM) coupled with energy dispersive spectrometry (EDX). Powders (Fe, Zn and Fe-Zn in a weight ratio of 1:1) were then compressed at the pressure of 545 MPa to the form of pellets with a diameter of 1.7 cm. Surface morphology and degradation behavior in the Hanks´ solution were studied and evaluated. Electrochemical polarization tests along with the static immersion tests carried out for 21 days were employed for corrosion behavior characterization. The highest corrosion rate was observed for pure Zn powder followed by the Fe-Zn and Fe, respectively. A mixed Fe-Zn sample showed similar properties as pure zinc with no signs of iron degradation after 21 days due to the effect of galvanic protection secured by the zinc acting as a sacrificial anode.
Carbon-Based Composites as Electrocatalysts for Oxygen Evolution Reaction in Alkaline Media
Authors: Stelmachowski Paweł , Duch Joanna , Sebastián David , Lázaro María Jesús , Kotarba Andrzej
Published: September 2021

This review paper presents the most recent research progress on carbon-based composite electrocatalysts for the oxygen evolution reaction (OER), which are of interest for application in low temperature water electrolyzers for hydrogen production. The reviewed materials are primarily investigated as active and stable replacements aimed at lowering the cost of the metal electrocatalysts in liquid alkaline electrolyzers as well as potential electrocatalysts for an emerging technology like alkaline exchange membrane (AEM) electrolyzers. Low temperature electrolyzer technologies are first briefly introduced and the challenges thereof are presented. The non-carbon electrocatalysts are briefly overviewed, with an emphasis on the modes of action of different active phases. The main part of the review focuses on the role of carbon–metal compound active phase interfaces with an emphasis on the synergistic and additive effects. The procedures of carbon oxidative pretreatment and an overview of metal-free carbon catalysts for OER are presented. Then, the successful synthesis protocols of composite materials are presented with a discussion on the specific catalytic activity of carbon composites with metal hydroxides/oxyhydroxides/oxides, chalcogenides, nitrides and phosphides. Finally, a summary and outlook on carbon-based composites for low temperature water electrolysis are presented.
Use of Ashes from Lignite Combustion as Fillers in Rubber Mixtures to Reduce VOC Emissions
Authors: Prochon Miroslawa , Bieliński Dariusz , Stepaniak Paulina , Makowicz Magdalena , Pietrzak Dominik , Dzeikala Oleksandra
Published: September 2021

This paper presents the use of ashes from brown coal combustion (BCA) as fillers in rubber mixtures, to reduce the emission of volatile organic compounds. Two types of ash, BCA1 and BCA2, were selected as fillers for styrene–butadiene rubber (SBR). The ashes were produced during the treatment of brown coal at the Bełchatów Power Plant in the years 2017 and 2018. The morphology and chemical composition of the ash were tested. Morphology studies using scanning microscopy showed differences in the grain sizes of the ashes, and EDS analysis showed a difference in their chemical compositions. Vulcanizates with different weight proportions of the individual ashes were produced. Mixtures were made with the addition of 10–30 pts. wt. ashes per 100 g of SBR. The addition of BCA1 ash at 10 and 30 pts. wt. reduced the emission of volatile organic compounds (VOC) while maintaining the good strength properties of the mixtures.
The Effect of Heat Flux to the Fire-Technical and Chemical Properties of Spruce Wood (Picea abies L.)
Authors: Zachar Martin , Čabalová Iveta , Kačíková Danica , Zacharová Lucia
Published: September 2021

The paper assesses the influence of the heat flux on spruce wood (Picea abies L.) behavior. The heat flux was performed at 15, 20, 25, and 30 kW·m−2. The fire-technical properties, such as the mass burning rate, charring thickness, charring rate, as well as the chemical composition (contents of the extractives, lignin, cellulose, holocellulose), of wood were determined. The highest burning rate of spruce wood of 0.32%·s−1 was reached at the heat flux of 30 kW·m−2. The charring rate ranged from 1.004 mm·min−1 (15 kW·m−2) to 2.016 mm·min−1 (30 kW·m−2). The proposed model of the charring process of spruce wood in time and appropriate thickness as a selected parameter is applicable in validation of the results of computer fire models in the design of fire protection of wooden buildings. The decrease in the holocellulose content mostly caused by the degradation of hemicelluloses was observed during thermal loading. The biggest decrease in hemicelluloses (24.94%) was recorded in samples loaded at 30 kW·m−2. The contents of cellulose increased due to the structural changes (carbonization and crosslinking), the content of lignin increased as well due to its higher thermal stability compared to saccharides, as well as the resulting lignin condensation.
Investigation on Control Burned of Bagasse Ash on the Properties of Bagasse Ash-Blended Mortars
Authors: Seyoum Redeat , Tesfamariam Belay Brehane , Andoshe Dinsefa Mensur , Algahtani Ali , Ahmed Gulam Mohammed Sayeed , Tirth Vineet
Published: September 2021

In recent years, partial replacement of cement with bagasse ash has been given attention for construction application due to its pozzolanic characteristics. Sugarcane bagasse ash and fine bagasse particles are abundant byproducts of the sugar industries and are disposed of in landfills. Our study presents the effect of burning bagasse at different temperatures (300 °C and 600 °C) on the compressive strength and physical properties of bagasse ash-blended mortars. Experimental results have revealed that bagasse produced more amorphous silica with very low carbon contents when it was burned at 600 °C/2 h. The compressive strength of mortar was improved when 5% bagasse ash replaced ordinary portland cement (OPC) at early curing ages. The addition of 10% bagasse ash cement also increased the compressive strength of mortars at 14 and 28 days of curing. However, none of the bagasse ash-blended portland pozzolana cement (PPC) mortars have shown improvement on compressive strength with the addition of bagasse ash. Characterization of bagasse ash was done using XRD, DTA-TGA, SEM, and atomic absorption spectrometry. Moreover, durability of mortars was checked by measuring water absorption and apparent porosity for bagasse ash-blended mortars.
Numerical Investigation into In-Plane Crushing of Tube-Reinforced Damaged 5052 Aerospace Grade Aluminum Alloy Honeycomb Panels
Authors: Djemaoune Younes , Krstic Branimir , Rasic Stefan , Radulovic Daniel , Dodic Marjan
Published: September 2021

This paper aims to investigate the crashworthiness performance degradation of a damaged 5052 aluminum honeycomb panels under in-plane uniaxial quasi-static compression and the possibility of improving it using reinforcement tubes. The in-plane crushing behaviors and energy absorption capacities of the intact, damaged, and tube-reinforced damaged panels with different damage sizes in both X1 and X2 directions are numerically simulated by using the nonlinear FE method Abaqus/Explicit, and the crashworthiness performances are compared with each other. The validation of finite element model involves comparing the obtained simulation results with theoretical and experimental ones. Very good agreement between numerical, experimental, and theoretical results is achieved. The first maximum compressive load and the mean crushing load of the different honeycomb configurations are analyzed and compared through the load–strain curves. The energy absorption capacity of the damaged and the tube-reinforced damaged panels is calculated and then compared with their corresponding intact ones. The deformation modes are explained in detail. The obtained results show that the crashworthiness performance degradation is directly proportional to the damage size as well as the insertion of reinforcement tubes considerably improves in-plane crushing resistance of damaged honeycomb panels.
A FEM-Based Optimization Method for Driving Frequency of Contactless Magnetoelastic Torque Sensors in Steel Shafts
Authors: Ostaszewska-Liżewska Anna , Nowicki Michał , Szewczyk Roman , Malinen Mika
Published: September 2021

This paper presents a novel finite element method (FEM) of optimization for driving frequency in magneto-mechanical systems using contactless magnetoelastic torque sensors. The optimization technique is based on the generalization of the axial and shear stress dependence of the magnetic permeability tensor. This generalization creates a new possibility for the determination of the torque dependence of a permeability tensor based on measurements of the axial stress on the magnetization curve. Such a possibility of quantitative description of torque dependence of a magnetic permeability tensor has never before been presented. Results from the FEM-based modeling method were validated against a real magnetoelastic torque sensor. The sensitivity characteristics of the model and the real sensor show a maximum using a driving current of similar frequency. Consequently, the proposed method demonstrates the novel possibility of optimizing magnetoelastic sensors for automotive and industrial applications.
Towards the High Phase Purity of Nanostructured Alluaudite-Type Glass-Ceramics Cathode Materials for Sodium Ion Batteries
Authors: Nowagiel Maciej , Samsel Mateusz J. , Pietrzak Tomasz K.
Published: September 2021

Alluaudite-type materials are systematically attracting more attention as prospective cathode materials for sodium ion batteries. In this paper, we strove to optimize various synthesis parameters of three alluaudite compositions (Na2Fe3(PO4)3—FFF, Na2VFe2(PO4)3—VFF, and Na2VFeMn(PO4)3—VFM) to obtain nanostructured alluaudite-type glass-ceramics with high phase purity. We systematically investigated the role of the chemical reactions, temperature and time of melting, cooling rate, and reducing factors on the quality of the final products. A detailed synthesis protocol along with X-ray diffractometry, thermal analysis, scanning electron microscopy imaging, and electrical conductivity measurements (with impedance spectroscopy) are reported. As a result, a significant increase of the conductivity was observed in the nanomaterials. The highest value was reached for the VFF composition and was equal to 6×10−4 S/cm at room temperature.
Learning Ecologies in the Digital Era: A Challenge for Primary Education in Low-Income Contexts
Authors: Moreno-Morilla Celia , Guzmán-Simón Fernando , García-Jiménez Eduardo
Published: September 2021

This research focused on the study of learning ecologies utilizing digital technology. The qualitative methodology used has allowed the analysis of the interactions children establish with digital technologies and the manner in which they construct a learning ecology. A total of 46 12-year-olds, their families, and their teachers participated in this study. The children’s schools and homes were in neighborhoods where structural situations of poverty and social and cultural marginality concurred. The children integrated researchers into their digital community, which allowed access to the events that the community was developing through digital technologies for two years. This information was complemented by the development of systematic observations and interviews with each participant. The participants’ multimodal linguistic and literacy practices were analyzed using a social semiotics approach. The results of the research describe and interpret the interactions that took place between participants and digital technologies. The research has identified the processes of recontextualization, transduction, and transcontextualization of the discourses developed in the frame of the participants’ learning ecologies. Digital ethnography has been revealed as an adequate method for studying learning ecologies.
Reliability Sampling Design for the Lifetime Performance Index of Gompertz Lifetime Distribution under Progressive Type I Interval Censoring
Authors: Wu Shu-Fei , Xie Yi-Jun , Liao Mao-Feng , Chang Wei-Tsung
Published: September 2021

In this artificial intelligence era, the constantly changing technology makes production techniques become sophisticated and complicated. Therefore, manufacturers are dedicated to improving the quality of products by increasing the lifetime in order to achieve the quality standards demanded by consumers. For products with lifetime following a Gompertz distribution, the lifetime performance index was used to measure the performance of manufacturing process under progressive type I interval censoring. The sampling design is investigated to reach the given level of significance and power level. When inspection interval length is fixed and the number of inspection intervals is not fixed, the required number of inspection intervals and sample size with minimum total cost are determined and tabulated. When the termination time is not fixed, the required number of inspection intervals, sample size, and equal interval length reaching the minimum total cost are determined and tabulated. The optimal parameter values are tabulated for the practical use of users. Finally, one practical example is given for the illustrative aim to show the implementation of this sampling design to collect data and the collected data are used to conduct the testing procedure to see if the process is capable.
Using Wolfram Alpha with Elementary Teacher Candidates: From More Than One Correct Answer to More Than One Correct Solution
Authors: Abramovich Sergei
Published: September 2021

The paper is a reflection on the author’s work with students enrolled in undergraduate and graduate elementary mathematics content and methods courses. Two specific pedagogical issues make up the focus of the paper. The first issue deals with demonstrating to future teachers the diversity of mathematical ideas behind a contextual question asked by a second-grade pupil allowing for multiple solution strategies to be used in addressing the question. The second issue deals with the use of Wolfram Alpha in aiding different mathematical features of this demonstration. The paper is congruous with mathematics teaching standards used across six continents and illustrated by reflective comments of the author’s students regarding their mathematics teacher education experiences. Teaching ideas shared in the paper may be of interest to instructors who want to explore elementary mathematics in depth with teacher candidates.
Biochemical Characterization of Cassiopea andromeda (Forsskål, 1775), Another Red Sea Jellyfish in the Western Mediterranean Sea
Authors: De Rinaldis Gianluca , Leone Antonella , De Domenico Stefania , Bosch-Belmar Mar , Slizyte Rasa , Milisenda Giacomo , Santucci Annalisa , Albano Clara , Piraino Stefano
Published: September 2021

Increasing frequency of native jellyfish proliferations and massive appearance of non-indigenous jellyfish species recently concur to impact Mediterranean coastal ecosystems and human activities at sea. Nonetheless, jellyfish biomass may represent an exploitable novel resource to coastal communities, with reference to its potential use in the pharmaceutical, nutritional, and nutraceutical Blue Growth sectors. The zooxanthellate jellyfish Cassiopea andromeda, Forsskål, 1775 (Cnidaria, Rhizostomeae) entered the Levant Sea through the Suez Canal and spread towards the Western Mediterranean to reach Malta, Tunisia, and recently also the Italian coasts. Here we report on the biochemical characterization and antioxidant activity of C. andromeda specimens with a discussion on their relative biological activities. The biochemical characterization of the aqueous (PBS) and hydroalcoholic (80% ethanol) soluble components of C. andromeda were performed for whole jellyfish, as well as separately for umbrella and oral arms. The insoluble components were hydrolyzed by sequential enzymatic digestion with pepsin and collagenase. The composition and antioxidant activity of the insoluble and enzymatically digestible fractions were not affected by the pre-extraction types, resulting into collagen- and non-collagen-derived peptides with antioxidant activity. Both soluble compounds and hydrolyzed fractions were characterized for the content of proteins, phenolic compounds, and lipids. The presence of compounds coming from the endosymbiont zooxanthellae was also detected. The notable yield and the considerable antioxidant activity detected make this species worthy of further study for its potential biotechnological sustainable exploitation.
Seaweed Protein Hydrolysates and Bioactive Peptides: Extraction, Purification, and Applications
Authors: Echave Javier , Fraga-Corral Maria , Garcia-Perez Pascual , Popović-Djordjević Jelena , H. Avdović Edina , Radulović Milanka , Xiao Jianbo , A. Prieto Miguel , Simal-Gandara Jesus
Published: September 2021

Seaweeds are industrially exploited for obtaining pigments, polysaccharides, or phenolic compounds with application in diverse fields. Nevertheless, their rich composition in fiber, minerals, and proteins, has pointed them as a useful source of these components. Seaweed proteins are nutritionally valuable and include several specific enzymes, glycoproteins, cell wall-attached proteins, phycobiliproteins, lectins, or peptides. Extraction of seaweed proteins requires the application of disruptive methods due to the heterogeneous cell wall composition of each macroalgae group. Hence, non-protein molecules like phenolics or polysaccharides may also be co-extracted, affecting the extraction yield. Therefore, depending on the macroalgae and target protein characteristics, the sample pretreatment, extraction and purification techniques must be carefully chosen. Traditional methods like solid–liquid or enzyme-assisted extraction (SLE or EAE) have proven successful. However, alternative techniques as ultrasound- or microwave-assisted extraction (UAE or MAE) can be more efficient. To obtain protein hydrolysates, these proteins are subjected to hydrolyzation reactions, whether with proteases or physical or chemical treatments that disrupt the proteins native folding. These hydrolysates and derived peptides are accounted for bioactive properties, like antioxidant, anti-inflammatory, antimicrobial, or antihypertensive activities, which can be applied to different sectors. In this work, current methods and challenges for protein extraction and purification from seaweeds are addressed, focusing on their potential industrial applications in the food, cosmetic, and pharmaceutical industries.
Intramuscular Boosting with hIFN-Alpha 2b Enhances BCGphipps-Induced Protection in a Murine Model of Leprosy
Authors: Guerrero Gloria G. , Rangel-Moreno Javier , Islas-Trujillo Sergio O. , Rojas-Espinosa Oscar
Published: September 2021

Host immunity to Mycobacterium leprae encompasses a spectrum of mechanisms that range from cellular immunity-driven protection to damage associated with humoral immunity as in type-2 leprosy reactions. Although type I interferons (IFNs) participate in eliminating intracellular pathogens, their contribution to the production of antibodies and CD3+ FOXP3+ regulatory T cells (Tregs) in BCG vaccine-mediated protection in leprosy is unknown. BCGphipps (BCGph) priming followed by intramuscular hIFN-α 2b boost significantly reduced lesion size and Mycobacterium lepraemurium growth in the skin. T follicular regulatory cells (TFR), a subset of Tregs induced by immunization or infection, reside in the germinal centers (GCs) and modulate antibody production. We found impaired Treg induction and improved GCs in draining lymph nodes of BCGph primed and hIFN-α 2b boosted mice. Moreover, these mice elicited significant amounts of IL-4 and IL-10 in serum. Thus, our results support the adjuvant properties of hIFN-α 2b in the context of BCGph priming to enhance protective immunity against skin leprosy.
Magnetic Properties of Bacterial Magnetosomes Produced by Magnetospirillum caucaseum SO-1
Authors: Gareev Kamil G. , Grouzdev Denis S. , Kharitonskii Peter V. , Kirilenko Demid A. , Kosterov Andrei , Koziaeva Veronika V. , Levitskii Vladimir S. , Multhoff Gabriele , Nepomnyashchaya Elina K. , Nikitin Andrey V. , Nikitina Anastasia , Sergienko Elena S. , Sukharzhevskii Stanislav M. , Terukov Evgeniy I. , Trushlyakova Valentina V. , Shevtsov Maxim
Published: September 2021

In this study, the magnetic properties of magnetosomes isolated from lyophilized magnetotactic bacteria Magnetospirillum caucaseum SO-1 were assessed for the first time. The shape and size of magnetosomes and cell fragments were studied by electron microscopy and dynamic light scattering techniques. Phase and elemental composition were analyzed by X-ray and electron diffraction and Raman spectroscopy. Magnetic properties were studied using vibrating sample magnetometry and electron paramagnetic resonance spectroscopy. Theoretical analysis of the magnetic properties was carried out using the model of clusters of magnetostatically interacting two-phase particles and a modified method of moments for a system of dipole–dipole-interacting uniaxial particles. Magnetic properties were controlled mostly by random aggregates of magnetosomes, with a minor contribution from preserved magnetosome chains. Results confirmed the high chemical stability and homogeneity of bacterial magnetosomes in comparison to synthetic iron oxide magnetic nanoparticles.
A Method for Rapid Screening, Expression, and Purification of Antimicrobial Peptides
Authors: Zhang Yingli , Li Zhongchen , Li Li , Rao Ben , Ma Lixin , Wang Yaping
Published: September 2021

In this study, a method for the rapid screening, expression and purification of antimicrobial peptides (AMPs) was developed. AMP genes were fused to a heat-resistant CL7 tag using the SLOPE method, and cloned into Escherichia coli and Pichia pastoris expression vectors. Twenty E. coli and ten P. pastoris expression vectors were constructed. Expression supernatants were heated, heteroproteins were removed, and fusion proteins were purified by nickel affinity (Ni-NTA) chromatography. Fusion proteins were digested on the column using human rhinovirus (HRV) 3C protease, and AMPs were released and further purified. Five AMPs (1, 2, 6, 13, 16) were purified using the E. coli expression system, and one AMP (13) was purified using the P. pastoris expression system. Inhibition zone and minimum inhibitory concentration (MIC) tests confirmed that one P. pastoris¬-derived and two E. coli-derived AMPs have the inhibition activity. The MIC of AMP 13 and 16 from E. coli was 24.2 μM, and the MIC of AMP 13 from P. pastoris was 8.1 μM. The combination of prokaryotic and eukaryotic expression systems expands the universality of the developed method, facilitating screening of a large number of biologically active AMPs, establishing an AMP library, and producing AMPs by industrialised biological methods.
A Reusable Efficient Green Catalyst of 2D Cu-MOF for the Click and Knoevenagel Reaction
Authors: Naskar Kaushik , Maity Suvendu , Maity Himadri Sekhar , Sinha Chittaranjan
Published: September 2021

[Cu(CPA)(BDC)]n (CPA = 4-(Chloro-phenyl)-pyridin-4-ylmethylene-amine; BDC = 1,4-benzenedicarboxylate) has been synthesized and structurally characterized by single crystal X-Ray diffraction measurement. The structural studies establish the copper (II) containing 2D sheet with (4,4) square grid structure. The square grid lengths are 10.775 and 10.769 Å. Thermal stability is assessed by TGA, and subsequent PXRD data establish the crystallinity. The surface morphology is evaluated by FE-SEM. The N2 adsorption−desorption analysis demonstrates the mesoporous feature (∼6.95 nm) of the Cu-MOF. This porous grid serves as heterogeneous green catalyst with superficial recyclability and thermal stability and facilitates organic transformations efficiently such as, Click and Knoevenagel reactions in the aqueous methanolic medium.
Distributions of Polychlorinated Naphthalenes in Sediments of the Yangtze River, China
Authors: Liu Zhitong , Xiao Ke , Wu Jingjing , Jia Tianqi , Lei Rongrong , Liu Wenbin
Published: September 2021

The pollution status of polychlorinated naphthalenes (PCNs) in the sediment of the Yangtze River Basin, Asia’s largest river basin, was estimated. The total concentrations of PCNs (mono- to octa-CNs) ranged from 0.103 to 1.631 ng/g. Mono-, di-, and tri-PCNs—consisting of CN-1, CN-5/7, and CN-24/14, respectively, as the main congeners—were the dominant homolog groups. Combustion indicators and principal component analysis showed that the emissions from halowax mixtures were the main contributor to PCNs in sediment, among most of the sampling sites. The mean total toxic equivalent (TEQ) was calculated to be 0.045 ± 0.077 pg TEQ/g, which indicates that the PCNs in sediments were of low toxicity to aquatic organisms. This work will expand the database on the distribution and characteristics of PCNs in the river sediment of China.
Synthesis and Biological Activity of a Cytostatic Inhibitor of MLLr Leukemia Targeting the DOT1L Protein
Authors: Bon Corentin , Si Yang , Pernak Melanie , Barbachowska Magdalena , Levi-Acobas Eva , Cadet Daniel Veronique , Jallet Corinne , Ruzic Dusan , Djokovic Nemanja , Djikić Teodora , Nikolic Katarina , Halby Ludovic , Arimondo Paola B.
Published: September 2021

Histone methyltransferase DOT1L catalyzes mono-, di- and trimethylation of histone 3 at lysine residue 79 (H3K79) and hypermethylation of H3K79 has been linked to the development of acute leukemias characterized by the MLL (mixed-lineage leukemia) rearrangements (MLLr cells). The inhibition of H3K79 methylation inhibits MLLr cells proliferation, and an inhibitor specific for DOT1L, pinometostat, was in clinical trials (Phase Ib/II). However, the compound showed poor pharmacological properties. Thus, there is a need to find new potent inhibitors of DOT1L for the treatment of rearranged leukemias. Here we present the design, synthesis, and biological evaluation of a small molecule that inhibits in the nM level the enzymatic activity of hDOT1L, H3K79 methylation in MLLr cells with comparable potency to pinometostat, associated with improved metabolic stability and a characteristic cytostatic effect.
Effect of Three Defatting Solvents on the Techno-Functional Properties of an Edible Insect (Gryllus bimaculatus) Protein Concentrate
Authors: Jeong Min-Soo , Lee Sang-Deok , Cho Seong-Jun
Published: September 2021

Edible insects have received global attention as an alternative protein-rich food. However, their structural characteristics make them difficult to digest. To overcome this obstacle, we assessed the techno-functional properties of three protein concentrates from the cricket Gryllus bimaculatus. Freeze-dried G. bimaculatus powder was defatted using ethanol, hexene, or acetone as solvents, and the techno-functional properties (protein solubility, water and oil holding capacity, foaming properties, emulsion capacity, and gel formation) of the protein concentrates were determined. Freeze-dried G. bimaculatus powder comprised approximately 17.3% crude fat and 51.3% crude protein based on dry weight. Ethanol was the most effective solvent for reducing the fat content (from 17.30% to 0.73%) and increasing the protein content (from 51.3% to 62.5%) of the concentrate. Techno-functionality properties drastically differed according to the defatting solvent used and foaming properties were most affected. Thus, the techno-functional and whole properties must be considered for proper application of edible insects to achieve global food sustainability.
Anticancer Effects of Ursi Fel Extract and Its Active Compound, Ursodeoxycholic Acid, in FRO Anaplastic Thyroid Cancer Cells
Authors: Jung Hyo Won , Hwang Ji Hye
Published: September 2021

Anaplastic thyroid cancer (ATC) is one of the most fatal human malignancies. Ursi Fel (UF) is the bile of a brown bear that has been traditionally used for heat clearance and toxin relief in Korean and Chinese medicines. In this study, we determined the anticancer effects of a UF extract and its active compound, ursodeoxycholic acid (UDCA), in FRO human ATC cells. FRO cells were treated with UF extract and UDCA at different concentrations for various durations. Cell viability was measured using an MTT assay. Cell apoptosis was investigated by flow cytometric analysis following Annexin V and propidium iodide (PI) staining, and Hoechst staining was used to observe nuclear fragmentation. The expression of pro-apoptotic (Bax, caspase-3, cytochrome c, and PARP), anti-apoptotic (Bcl-2), and angiogenetic (TGF-β, VEGF, N-cadherin, and sirtuin-1) proteins and the phosphorylation of Akt and mechanistic target of rapamycin (mTOR) were determined by western blot analysis. Treatment with UF extract at 10, 25, and 50 μg/mL and UDCA at 25, 50, and 100 μM/mL significantly inhibited the growth of FRO cells in a dose-dependent manner. Flow cytometry and Hoechst staining revealed an increase in the apoptosis of FRO cells mediated by UF extract and UDCA in a dose-dependent manner. UF extract (25 and 50 μg) and UDCA (50 and 100 μM) significantly increased the expression of Bax, caspase-3, cytochrome c, and PARP and inhibited the expression of Bcl-2, TGF-β, VEGF, N-cadherin, and sirtuin-1 in FRO cells. Furthermore, UF extract and UDCA treatment stimulated Akt phosphorylation and inhibited mTOR phosphorylation in these cells. These results indicate that UF extract and UDCA exert anticancer properties in FRO cells by inducing apoptosis and inhibiting angiogenesis via regulating the Akt/mTOR signaling pathway.
Serum Proteomic Analysis of Cannabis Use Disorder in Male Patients
Authors: Alasmari Fawaz , Alsanea Sary , Alfadda Assim A. , Alanazi Ibrahim O. , Musambil Mohthash , Masood Afshan , Alqahtani Faleh , Fantoukh Omer I. , Alasmari Abdullah F. , Benabdelkamel Hicham
Published: September 2021

Cannabis use has been growing recently and it is legally consumed in many countries. Cannabis has a variety of phytochemicals including cannabinoids, which might impair the peripheral systems responses affecting inflammatory and immunological pathways. However, the exact signaling pathways that induce these effects need further understanding. The objective of this study is to investigate the serum proteomic profiling in patients diagnosed with cannabis use disorder (CUD) as compared with healthy control subjects. The novelty of our study is to highlight the differentially changes proteins in the serum of CUD patients. Certain proteins can be targeted in the future to attenuate the toxicological effects of cannabis. Blood samples were collected from 20 male individuals: 10 healthy controls and 10 CUD patients. An untargeted proteomic technique employing two-dimensional difference in gel electrophoresis coupled with mass spectrometry was employed in this study to assess the differentially expressed proteins. The proteomic analysis identified a total of 121 proteins that showed significant changes in protein expression between CUD patients (experimental group) and healthy individuals (control group). For instance, the serum expression of inactive tyrosine protein kinase PEAK1 and tumor necrosis factor alpha-induced protein 3 were increased in CUD group. In contrast, the serum expression of transthyretin and serotransferrin were reduced in CUD group. Among these proteins, 55 proteins were significantly upregulated and 66 proteins significantly downregulated in CUD patients as compared with healthy control group. Ingenuity pathway analysis (IPA) found that these differentially expressed proteins are linked to p38MAPK, interleukin 12 complex, nuclear factor-κB, and other signaling pathways. Our work indicates that the differentially expressed serum proteins between CUD and control groups are correlated to liver X receptor/retinoid X receptor (RXR), farnesoid X receptor/RXR activation, and acute phase response signaling.
Chemical Profile, In Vitro Biological Activity and Comparison of Essential Oils from Fresh and Dried Flowers of Lavandula angustifolia L.
Authors: Caprari Claudio , Fantasma Francesca , Divino Fabio , Bucci Antonio , Iorizzi Maria , Naclerio Gino , Ranalli Giancarlo , Saviano Gabriella
Published: September 2021

The chemical composition of essential oils (EOs) from dried and fresh flowers of Lavandula angustifolia L. (lavender), named LA 2019 and LA 2020, respectively, grown in central Italy was analyzed and compared by GC and GC-MS. For both samples, 61 compounds were identified, corresponding to 97.9% and 98.1% of the total essential oils. Explorative data analysis, performed to compare the statistical composition of the samples, resulted in a high level of global similarity (around 93%). The compositions of both samples were characterized by 10 major compounds, with a predominance of Linalool (35.3–36.0%), Borneol (15.6–19.4%) and 1,8-Cineole (11.0–9.0%). The in vitro antibacterial activity assay by disk diffusion tests against Bacillus subtilis PY79 and Escherichia coli DH5α showed inhibition of growth in both indicator strains. In addition, plate counts revealed a bactericidal effect on E. coli, which was particularly noticeable when using oil from the fresh lavender flowers at the highest concentrations. An in vitro antifungal assay showed that the EOs inhibited the growth of Sclerotium rolfsii, a phytopathogenic fungus that causes post-harvest diseases in many fruits and vegetables. The antioxidant activity was also assessed using the ABTS free radical scavenging assay, which showed a different antioxidant activity in both EOs. In addition, the potential application of EOs as a green method to control biodeterioration phenomena on an artistic wood painting (XIX century) was evaluated.
Amorphous Form of Carvedilol Phosphate—The Case of Divergent Properties
Authors: Sip Szymon , Rosiak Natalia , Miklaszewski Andrzej , Talarska Patrycja , Dudziec Ewa , Cielecka-Piontek Judyta
Published: September 2021

The amorphous form of carvedilol phosphate (CVD) was obtained as a result of grinding. The identity of the obtained amorphous form was confirmed by powder X-ray diffraction (PXRD), different scanning calorimetry (DSC), and FT-IR spectroscopy. The process was optimized in order to obtain the appropriate efficiency and time. The crystalline form of CVD was used as the reference standard. Solid dispersions of crystalline and amorphous CVD forms with hydrophilic polymers (hydroxypropyl-β-cyclodextrin, Pluronic® F-127, and Soluplus®) were obtained. Their solubility at pH 1.2 and 6.8 was carried out, as well as their permeation through a model system of biological membranes suitable for the gastrointestinal tract (PAMPA-GIT) was established. The influence of selected polymers on CVD properties was defined for the amorphous form regarding the crystalline form of CVD. As a result of grinding (four milling cycles lasting 15 min with 5 min breaks), amorphous CVD was obtained. Its presence was confirmed by the “halo effect” on the diffraction patterns, the disappearance of the peak at 160.5 °C in the thermograms, and the changes in position/disappearance of many characteristic bands on the FT-IR spectra. As a result of changes in the CVD structure, its lower solubility at pH 1.2 and pH 6.8 was noted. While the amorphous dispersions of CVD, especially with Pluronic® F-127, achieved better solubility than combinations of crystalline forms with excipients. Using the PAMPA-GIT model, amorphous CVD was assessed as high permeable (Papp > 1 × 10−6 cm/s), similarly with its amorphous dispersions with excipients (hydroxypropyl-β-cyclodextrin, Pluronic® F-127, and Soluplus®), although in their cases, the values of apparent constants permeability were decreased.
Imidazopyridazine Acetylcholinesterase Inhibitors Display Potent Anti-Proliferative Effects in the Human Neuroblastoma Cell-Line, IMR-32
Authors: Sharma Rakesh Kumar , Singh Manisha , Ghimeray Khagendra , Juneja Pinky , Dev Gagan , Pulavarthi Sridhar , Reddy Sabbasani Rajasekhara , Akundi Ravi Shankar
Published: September 2021

Imidazo[1,2-b]pyridazine compounds are a new class of promising lead molecules to which we have incorporated polar nitro and amino moieties to increase the scope of their biological activity. Two of these substituted 3-nitro-6-amino-imidazo[1,2-b]pyridazine compounds (5c and 5h) showed potent acetylcholinesterase (AChE) inhibitory activity (IC50 40–50 nM), which we have previously reported. In this study, we wanted to test the biological efficacy of these compounds. Cytotoxicity assays showed that compound 5h mediated greater cell death with over 43% of cells dead at 100 μM and activation of caspase 3-mediated apoptosis. On the other hand, compound 5c mediated a dose-dependent decrease in cell proliferation. Both compounds showed cell cycle arrest in the G0/G1 phase and reduced cellular ATP levels leading to activation of adenosine monophosphate-activated protein kinase (AMPK) and enhanced mitochondrial oxidative stress. It has to be noted that all these effects were observed at doses beyond 10 μM, 200-fold above the IC50 for AChE inhibition. Both compounds also inhibited bacterial lipopolysaccharide-mediated cyclooxygenase-2 and nitric oxide release in primary rat microglial cells. These results suggested that the substituted imidazo (1,2-b) pyridazine compounds, which have potent AChE inhibitory activity, were also capable of antiproliferative, anti-migratory, and anti-inflammatory effects at higher doses.
Time Mating Guinea Pigs by Monitoring Changes to the Vaginal Membrane throughout the Estrus Cycle and with Ultrasound Confirmation
Authors: Wilson Rebecca L. , Lampe Kristin , Matushewski Brad J. , Regnault Timothy R. H. , Jones Helen N.
Published: September 2021

One of the greatest challenges to the development and implementation of pregnancy therapeutics is the ability to rigorously test treatments in clinically relevant animal models. Guinea pigs offer a unique advantage in studying the placenta, fetal development, and reproductive health as they have similar developmental milestones to humans, both throughout gestation and following birth. Tracking the guinea pig estrus cycle is imperative to ensuring appropriately timed mating and can be performed by monitoring the guinea pig vaginal membrane. Here, we describe a methodology to efficiently and accurately time mate guinea pigs, and provide a picture representation of changes to the guinea pig vaginal membrane throughout the estrus cycle. Utilization of this monitoring enabled a 100% pregnancy success rate on the first mating attempt in a cohort of five guinea pigs. This approach, along with early pregnancy ultrasounds as a secondary method to confirm pregnancy, offers a reliable approach to timed mating in the guinea pig.
Cluster Randomized Controlled Trial on the Effects of 12 Months of Combined Exercise Training during Hemodialysis in Patients with Chronic Kidney Disease—Study Protocol of the Dialysis Training Therapy (DiaTT) Trial
Authors: von Gersdorff Gero , von Korn Pia , Duvinage André , Ihorst Gabriele , Josef Anika , Kaufmann Margit , Baer Thomas , Fellerhoff Tim , Fuhrmann Iris , Koesel Elisa , Zeissler Sven , Bobka Lars , Heinrich Marion , Schindler Anette , Weber Rasmus , Breuer Cornelia , Meyer Anna Maria , Polidori M. Cristina , Dinges Sophia M.T. , Schoenfeld Julia , Siebenbuerger Mathias , Degenhardt Stefan , Anding-Rost Kirsten , Halle Martin
Published: September 2021

Patients with chronic kidney disease (CKD) on hemodialysis (HD) experience treatment-related immobility and physical deconditioning, which is responsible for an increased risk of frailty and a high burden of multi-morbidity. Exercise has been shown to counteract this vicious cycle; however, its effectiveness has only been investigated in small cohorts. Therefore, the objective of the Dialysis Training Therapy (DiaTT) trial will be to assess the effects of a 12-month intradialytic exercise program on physical functioning, frailty and health economics in a large cohort of HD patients in a real-world setting. DiaTT will be a prospective, cluster-randomized (1:1), controlled, multi-center, interventional clinical trial across 28 dialysis units, aiming at the recruitment of >1100 CKD patients on HD. The intervention group will receive 12 months’ intradialytic exercise (combined aerobic and resistance training), whereas the usual care group will not receive intervention. The primary endpoint will be a change on the sit-to-stand test (STS60) result between baseline and 12 months. Secondary endpoints will include physical functioning, frailty, quality of life, 3-point MACE, hospitalizations, survival, quality of HD, health literacy and health care costs. By including almost as many patients as previously investigated in smaller trials, DiaTT will be the largest randomized, controlled trial assessing frailty, quality of life and mortality in the field of nephrology.