bluuebunny/crossref_metadata_2025 huggingface dataset:
Conflict, Diaspora, and Empire
Authors: Gannon Darragh
Published: June 2023

The actions of Irish nationalists in Britain are often characterised as a 'sideshow' to the revolutionary events in Ireland between 1912 and 1922. This original study argues, conversely, that Irish nationalism in Britain was integral to contemporary Irish and British assessments of the Irish Revolution between the Third Home Rule Bill and the Anglo-Irish Treaty. Darragh Gannon charts the development of Irish nationalism across the Irish Sea over the course of a historic decade in United Kingdom history – from constitutional crisis, to war, and revolution. The book documents successive Home Rule and IRA campaigns in Britain coordinated by John Redmond and Michael Collins respectively and examines the mobilisation of Irish migrant communities in British cities in response to major political crises, from the Ulster crisis to the First World War. Finally, Conflict, Diaspora, and Empire assesses the impacts of Irish nationalism in metropolitan Britain, from Whitehall to Westminster. The Irish Revolution, this study concludes, was defined by political conflicts, and cultures, across the Irish Sea.
Local Data Spaces: Leveraging trusted research environments for secure location-based policy research in the age of coronavirus disease-2019
Authors: Macdonald Jacob L. , Green Mark A. , Gibin Maurizio , Leech Simon , Singleton Alex , Longley Paul
Published: June 2023

This work explores the use of Trusted Research Environments for the secure analysis of sensitive, record-level data on local coronavirus disease-2019 (COVID-19) inequalities and economic vulnerabilities. The Local Data Spaces (LDS) project was a targeted rapid response and cross-disciplinary collaborative initiative using the Office for National Statistics’ Secure Research Service for localized comparison and analysis of health and economic outcomes over the course of the COVID-19 pandemic. Embedded researchers worked on co-producing a range of locally focused insights and reports built on secure secondary data and made appropriately open and available to the public and all local stakeholders for wider use. With secure infrastructure and overall data governance practices in place, accredited researchers were able to access a wealth of detailed data and resources to facilitate more targeted local policy analysis. Working with data within such infrastructure as part of a larger research project involved advanced planning and coordination to be efficient. As new and novel granular data resources become securely available (e.g., record-level administrative digital health records or consumer data), a range of local policy insights can be gained across issues of public health or local economic vitality. Many of these new forms of data however often come with a large degree of sensitivity around issues of personal identifiability and how the data is used for public-facing research and require secure and responsible use. Learning to work appropriately with secure data and research environments can open up many avenues for collaboration and analysis.
Sinh-arcsinh-normal distributions to add uncertainty to neural network regression tasks: Applications to tropical cyclone intensity forecasts
Authors: Barnes Elizabeth A. , Barnes Randal J. , DeMaria Mark
Published: June 2023

A simple method for adding uncertainty to neural network regression tasks in earth science via estimation of a general probability distribution is described. Specifically, we highlight the sinh-arcsinh-normal distributions as particularly well suited for neural network uncertainty estimation. The methodology supports estimation of heteroscedastic, asymmetric uncertainties by a simple modification of the network output and loss function. Method performance is demonstrated by predicting tropical cyclone intensity forecast uncertainty and by comparing two other common methods for neural network uncertainty quantification (i.e., Bayesian neural networks and Monte Carlo dropout). The simple approach described here is intuitive and applicable when no prior exists and one just wishes to parameterize the output and its uncertainty according to some previously defined family of distributions. The authors believe it will become a powerful, go-to method moving forward.
Is a baseball like knuckleball possible in cricket?
Authors: Shah Kunjal , Mittal Sanjay
Published: June 2023

The knuckleball is considered to be one of the hardest pitches to hit in baseball due to its seemingly unpredictable motion. It has gained popularity in cricket in recent times. It is shown that the delivery referred to as knuckleball in cricket, at present, does not exhibit a zigzag motion and is, therefore, a misnomer. We propose a delivery in cricket that is associated with an erratic trajectory similar to the knuckleball pitch in baseball. Force measurement experiments in a wind tunnel on a new cricket ball in various orientations of the seam to the incoming flow and at different Reynolds number are carried out. The results are utilized to estimate the trajectory of knuckleball deliveries. The key parameters are the seam angle, speed and spin rate of the ball at the time of its release. Their effect on the trajectory is studied in detail. The optimal combination of these parameters that result in a knuckleball, which is likely hard for the batter to play, is identified.
Perspectives of psychiatric trainees and examiners on the assessment of communication skills during an online clinical examination: a qualitative study
Authors: Usman Memoona , Adamis Dimitrios , McCarthy Geraldine
Published: June 2023

Background:

Effective doctor–patient communication is a core competency for healthcare professionals. With the pivot to online clinical education and assessment due to the COVID-19 pandemic, there was a need to explore the views of psychiatric trainees and examiners on assessment of communication skills during online high stakes postgraduate examinations.

Methods:

The study was designed as descriptive qualitative research. All candidates and examiners of the September and November 2020 sitting of online Basic Specialist Training exam (a clinical Objective Structured Clinical Examination exam completed in the first 4 years of psychiatry training) were invited to participate. The respondents were interviewed by Zoom which was transcribed verbatim. Data were analyzed by NVivo20 pro and various themes and subthemes were drawn using Braun and Clarke thematic analysis.

Results:

A total of seven candidates and seven examiners were interviewed with an average duration of 30 minutes and 25 minutes, respectively. Four main themes emerged: Communication, Screen optimization, Continuation postpandemic and Overall experience. All candidates preferred to continue an online format post pandemic for practical reasons e.g., avoiding travel and overnight stay, while all examiners preferred to go back to in-person Objective Structured Clinical Examination. However, continuation of online Clinical Formulation and Management Examination was agreed by both groups.

Conclusion:

The participants were largely satisfied with the online examination but did not consider it equal to face-to-face for picking up nonverbal cues. Overall minimal technical issues were reported. These findings may be helpful to modify current psychiatry membership examinations or similar assessments in other countries and specialties.
Extrauterine growth restriction and low energy intake during the early neonatal period of very low birth weight infants are associated with decreased lung function in childhood
Authors: Uberos-Fernández Jose , Ruiz-López Aida , Carrasco-Solis Marta , Fernandez-Marín Elizabeth , Garcia-Cuesta Aida , Campos-Martínez Ana
Published: June 2023

Premature birth, bronchopulmonary dysplasia or restrictive nutrition in the first weeks of postnatal life may have repercussions on lung development and affect long-term lung function outcomes. This prospective observational study is based on a cohort of 313 very low birth weight (VLBW) neonates, born between 1 January 2008 and 1 December 2016. The daily intake of calories, protein, fat and carbohydrates during the first week of life and evidence of inadequate weight gain (Δwt) until week 36 of gestational age (GA) were recorded. FEV1, FEF25–75 %, forced vital capacity (FVC) and the FEV1/FVC ratio were determined. The relations between these parameters were determined by regression analysis. Spirometric parameters were obtained for 141 children with a mean age of 9 years (95 % CI 7, 11); 69 of them (48·9 %) had presented wheezing episodes on more than three occasions. In addition, 60 (42·5 %) had a history of bronchopulmonary dysplasia. Of these, n 40 (66·6 %) had a history of wheezing. Significant association between protein/energy intake in the first week of life and the lung function parameters analysed was observed. Poor Δwt to GA week 36 was significantly associated with decreased mean pulmonary flow. Inadequate protein/energy intake in the first week of life of VLBW newborns and poor Δwt to week 36 of GA is associated with a significant worsening of lung function parameters.
Media Sentiment and Currency Reversals
Authors: Filippou Ilias , Taylor Mark P. , Wang Zigan
Published: June 2023

Analyzing 48 foreign exchange (FX) rates and 1.2 million FX-related news articles over a 35-year period, using digital textual analysis, we find that a currency reversal investment strategy that buys (sells) currencies with low (high) media sentiment offers strong positive and statistically significant returns and Sharpe ratios. The results are robust and the strategy adds value over other currency premia determinants. Analysts’ forecasts systematically mispredict the reversal strategy. This is the first article to show that price reversals based on media sentiment are a well-defined feature of the FX market.
A review study on the design and control of optimised greenhouse environments
Authors: Chimankare Renuka Vinod , Das Subhra , Kaur Karamjit , Magare Dhiraj
Published: June 2023

Greenhouses are inflated structures with transparent covering that are used to grow crops under controlled climatic conditions. Crops are protected from extreme climate-related events by being enclosed. Furthermore, the greenhouse design ratio impacts the temperature and humidity distribution profile uniformity as well as the greenhouse. As a result, by effectively designing the greenhouse structure, building materials, dimensions, and shapes, the cost of cooling management strategies can be reduced. Structures with changed arch shapes showed to be more effective at reducing greenhouse cooling demands in hot areas. To demonstrate the tropical region’s inherent capabilities for generating a proper atmosphere for plant development, the optimal temperature, humidity, light, and PH for greenhouse production of crops were supplied. Greenhouse cooling systems are dominated by local environmental characteristics that have an immediate impact on their indoor climatic conditions. Photovoltaic systems in greenhouses have proven technological capacity in real-world settings in this area. This could increase the energy efficiency of some agrivoltaic greenhouse design options.
From “surviving to thriving”: Mood Lifters – a wellness program for parents of medically complex children
Authors: Dews Aridenne A. , Pokowitz Elena L. , Votta Cecilia M. , Yan Haoyang , Pituch Kenneth , Deldin Patricia J.
Published: June 2023

Objectives

Parents of medically complex children juggle unique demands associated with caring for chronically ill children, many of which negatively impact their mental wellbeing. Despite this, parents of medically complex children often forgo mental health support due to concerns with costs, time, stigma, and accessibility. There is limited research on evidence-based interventions addressing such barriers for these caregivers. We piloted an adapted version of Mood Lifters, a peer-led wellness program, to equip parents of medically complex children with evidence-based strategies to manage their mental health while also reducing barriers to support. We hypothesized parents would find Mood Lifters to be feasible and acceptable. Further, parents would experience improvements in mental wellbeing upon program completion.

Methods

We conducted a single-arm prospective pilot study to assess Mood Lifters for parents of medically complex children. Participants included 51 parents in the U.S. recruited from a local pediatric hospital providing care for their children. Caregiver mental wellbeing was assessed through validated questionnaires pre-intervention (T1) and post-intervention (T2). Repeated-measures analysis of variance was conducted to evaluate change between T1 and T2.

Results

Analyses from T1 and T2 (n = 18) revealed improvements in parents’ depression (F(1,17) = 7.691, p = 0.013) and anxiety (F(1,17) = 6.431, p = 0.021) after program completion. Improvements in perceived stress and positive and negative emotion were significant at p < 0.0083.

Significance of results

Parents of medically complex children experienced improved mental health upon participating in Mood Lifters. Results offer preliminary support for the feasibility and acceptability of Mood Lifters as an evidence-based care option that may also alleviate common barriers to care.
To (i)B or not to i(B), that is the question: on the differences between Ellis’ REBT and Beck’s CT
Authors: Young P. , Turner M. J.
Published: June 2023

Far from being a monolithic approach to psychotherapy, cognitive behavioural therapy (CBT) is in fact an umbrella term to describe a family of psychological therapies that share many common features but also have nuanced differences. Of the CBTs, two are often conflated under the ‘CBT’ moniker, namely cognitive therapy (CT) and rational emotive behaviour therapy (REBT). In this article, we explore some of the key differences and similarities between CT and REBT, touching on philosophy, practical implementation, and literature. We provide a brief hypothetical case study to demonstrate the different ways a therapist using CT and REBT might tackle the same client problem. We do not declare either approach superior, but suggest each might have their advantages in certain contexts and acknowledge that skilful practitioners could, and often do, integrate both approaches. As CBT continues to evolve and move into new areas, it is important that psychology practitioners and researchers are clear about which specific approach to CBT they are delivering, measuring and/or reporting on.
Can we interrogate public databases to fill critical gaps in mental health epidemiology? Testing the association between cannabis and psychosis in the UK as an example
Authors: Di Gennaro Gianfranco , Colizzi Marco
Published: June 2023

The psychoactive properties of cannabis have been known forever. Since 1987, several prospective studies have suggested an increased risk of psychosis among cannabis users, with alternative explanations failing to account for such an effect. A cause–effect relationship has thus been implied. Further evidence has indicated that there is a dose–response relationship, and high-potency cannabis varieties confer the greatest risk of psychosis. As cannabis use has become more common over the last decades, one would expect a related increase in the number of schizophrenia cases. However, evidence in this regard remains equivocal for several reasons, including relying on databases that are not primarily designed to address such question and the issue that solid information regarding the incidence of schizophrenia is a relatively recent acquisition. Recent years have seen the development of online web publications, such as Google Trends and “Our World in Data”, where data are explorable and interactable for tracking and comparing trends over specific periods and world regions. By using such databases, we believe that the question whether changes in cannabis use are associated with changes in schizophrenia rates can be answered, at least partly. Therefore, we tested these tools by evaluating trends in cannabis use and both cases and prevalence of schizophrenia in the United Kingdom, one of the countries where the incident rates for psychotic disorder have been suggested to be particularly increased by cannabis consumption. Crossing data from these tools revealed that interest in cannabis has been growing at the country level for over 10 years, with a parallel overlapping raise in psychosis cases and prevalence. Following up on this example, let us think of how many public health opportunities these public resources may offer. The question now is whether public health interventions for the benefit of the general population will follow suit.
Bright-field to fluorescence microscopy image translation for cell nuclei health quantification
Authors: Wang Ruixiong , Butt Daniel , Cross Stephen , Verkade Paul , Achim Alin
Published: June 2023

Microscopy is a widely used method in biological research to observe the morphology and structure of cells. Amongst the plethora of microscopy techniques, fluorescent labeling with dyes or antibodies is the most popular method for revealing specific cellular organelles. However, fluorescent labeling also introduces new challenges to cellular observation, as it increases the workload, and the process may result in nonspecific labeling. Recent advances in deep visual learning have shown that there are systematic relationships between fluorescent and bright-field images, thus facilitating image translation between the two. In this article, we propose the cross-attention conditional generative adversarial network (XAcGAN) model. It employs state-of-the-art GANs (GANs) to solve the image translation task. The model uses supervised learning and combines attention-based networks to explore spatial information during translation. In addition, we demonstrate the successful application of XAcGAN to infer the health state of translated nuclei from bright-field microscopy images. The results show that our approach achieves excellent performance both in terms of image translation and nuclei state inference.
Water Supersaturation for Early Mars
Authors: Delavois A. , Forget F. , Turbet M. , Millour E. , Vandemeulebrouck R. , Lange L. , Bierjon A.
Published: June 2023

Evidence of past liquid water flowing on the surface of Mars has been identified since the first orbital mission to the planet. However, reconstructing the climate that would allow liquid water at the surface is still an intense area of research. Previous studies showed that an atmosphere composed only of CO2 and H2O could not sustain surface temperatures above the freezing point of water. Different solutions have been studied, ranging from events like impacts on different atmospheric compositions, or even radiative feedback of water clouds that would create a dramatic greenhouse effect. In this context, we propose to study whether the supersaturation of water could warm the planet. Strong supersaturation is observed in the present‐day Martian atmosphere. On early Mars, supersaturation could enhance the greenhouse effect through strong absorption of the IR flux by water vapor or by modifying water clouds. While 1D modeling suggests a significant impact, our 3D model shows that warming the climate of early Mars requires a high supersaturation ratio, especially in the lower layers of the atmosphere. This configuration seems highly unrealistic since the level of supersaturation is higher than what would be expected in a dense atmosphere.
Simultaneous Inversion of Ocean Bottom Pressure and Electromagnetic Tsunami Records for the 2009 Samoa Earthquake
Authors: Yokoi Hiiro , Baba Toshitaka , Lin Zhiheng , Minami Takuto , Kamiya Masato , Naitoh Akino , Toh Hiroaki
Published: June 2023

This study presents tsunami inversion analyses that simultaneously use ocean‐bottom pressure and ocean‐bottom electromagnetic data along with an accurate tsunami propagation model to estimate the earthquake source model for the 2009 Samoa earthquake. This earthquake was a doublet event that occurred along a pair of normal and thrust faults. To the best of our knowledge, this joint inversion of pressure and electromagnetic data is unprecedented. The incorporation of electromagnetic data in the tsunami inversion analysis was relatively simple, and the inclusion of frequency dispersion, self‐attraction, and loading effects in the tsunami propagation calculations allowed the use of tsunami waveforms recorded at distant locations, which improved the reliability of the solutions obtained. Our best source model indicated that the east‐west extent of the plate‐boundary slip area was approximately 50 km, which is narrower than that of a previous fault model, and explained both electromagnetic and pressure data from the 2009 Samoa tsunami.
Poleward Shift in the Southern Hemisphere Westerly Winds Synchronous With the Deglacial Rise in CO2
Authors: Gray William R. , de Lavergne Casimir , Jnglin Wills Robert C. , Menviel Laurie , Spence Paul , Holzer Mark , Kageyama Masa , Michel Elisabeth
Published: June 2023

The Southern Hemisphere westerly winds influence deep ocean circulation and carbon storage. While the westerlies are hypothesized to play a key role in regulating atmospheric CO2 over glacial‐interglacial cycles, past changes in their position and strength remain poorly constrained. Here, we use a compilation of planktic foraminiferal δ18O from across the Southern Ocean and emergent relationships within an ensemble of climate models to reconstruct changes in the Southern Hemisphere surface westerlies over the last deglaciation. We infer a 4.8° (2.9–7.1°, 95% confidence interval) equatorward shift and about a 25% weakening of the westerlies during the Last Glacial Maximum (20 ka) relative to the mid‐Holocene (6.5 ka). Climate models from the Palaeoclimate Modeling Intercomparison Project substantially underestimate this inferred equatorward wind shift. According to our reconstruction, the poleward shift in the westerlies over deglaciation closely mirrors the rise in atmospheric CO2 (R2 = 0.98). Experiments with a 0.25° resolution ocean‐sea‐ice‐carbon model suggest that shifting the westerlies equatorward reduces the overturning rate of the ocean below 2 km depth, leading to a suppression of CO2 outgassing from the polar Southern Ocean. Our results support a role for the westerly winds in driving the deglacial CO2 rise, and suggest outgassing of natural CO2 from the Southern Ocean is likely to increase as the westerlies shift poleward due to anthropogenic warming.
Development and Testing of a Digitally Enabled Adherence Platform
Authors: Abelson Elana , Hooper David , Varnell Charles , Streat Derek , Sharma Gunjan , Gavandi Sid , Modi Avani C.
Published: June 2023

Objective:

To develop a digitally enabled adherence platform (DEAP) using evidence-based adherence promotion strategies through iterative testing with patients, design specialists, and adherence experts.

Method:

Adolescents and young adults (AYAs) with a kidney transplant from a large, midwestern transplant program were recruited to participate in this study. Through an iterative process, we conducted focus groups, individual interviews, empathy interviews/mapping, and usability testing and evaluation to develop and refine the initial version of DEAP.

Results:

Three empathy maps were created to assess the unique needs of patients, caregivers, and healthcare providers. Eleven patients were then recruited to participate in usability testing of the platform. Platform engagement was high with nine of the 11 participants viewing their initial messages/surveys, usability was rated as high ( M = 81.88, SD = 9.61, range = 67.5–95.0), and user-friendliness was rated as excellent ( M = 5.88, SD = 0.74, range = 5.0–7.0). Participants also noted multiple opportunities for improving the look and design of DEAP.

Conclusions:

Engaging with multiple stakeholders and piloting a small usability study have demonstrated support for the acceptability and usability of DEAP, a mobile health platform to address barriers to taking immunosuppression medication for AYAs who have received kidney transplants. Future work is planned to develop a better patient and clinician user experience, increase personalization, and facilitate patient–clinician engagement using this platform.

Implications for Impact Statement

Up to 40% of adolescents and young adults (AYAs) who have undergone kidney transplants do not take their immunosuppression medication as prescribed, which can lead to devastating consequences including organ rejection. DEAP (digitally enabled adherence platform), a web-based system, was designed using an iterative process with input from key stakeholders with features to assess adherence barriers and provide solutions through problem-solving. Preliminary usability testing suggests it is acceptable and easy to use, demonstrating its potential to address suboptimal adherence and improve health outcomes.
Ultra-thin light-weight laser-induced-graphene (LIG) diffractive optics
Authors: Lee Younggeun , Low Mun Ji , Yang Dongwook , Nam Han Ku , Le Truong-Son Dinh , Lee Seung Eon , Han Hyogeun , Kim Seunghwan , Vu Quang Huy , Yoo Hongki , Yoon Hyosang , Lee Joohyung , Sandeep Suchand , Lee Keunwoo , Kim Seung-Woo , Kim Young-Jin
Published: June 2023

The realization of hybrid optics could be one of the best ways to fulfill the technological requirements of compact, light-weight, and multi-functional optical systems for modern industries. Planar diffractive lens (PDL) such as diffractive lenses, photonsieves, and metasurfaces can be patterned on ultra-thin flexible and stretchable substrates and be conformally attached on top of arbitrarily shaped surfaces. In this review, we introduce recent research works addressed to the design and manufacturing of ultra-thin graphene optics, which will open new markets in compact and light-weight optics for next-generation endoscopic brain imaging, space internet, real-time surface profilometry, and multi-functional mobile phones. To provide higher design flexibility, lower process complexity, and chemical-free process with reasonable investment cost, direct laser writing (DLW) of laser-induced-graphene (LIG) is actively being applied to the patterning of PDL. For realizing the best optical performances in DLW, photon-material interactions have been studied in detail with respect to different laser parameters; the resulting optical characteristics have been evaluated in terms of amplitude and phase. A series of exemplary laser-written 1D and 2D PDL structures have been actively demonstrated with different base materials, and then, the cases are being expanded to plasmonic and holographic structures. The combination of these ultra-thin and light-weight PDL with conventional bulk refractive or reflective optical elements could bring together the advantages of each optical element. By integrating these suggestions, we suggest a way to realize the hybrid PDL to be used in the future micro-electronics surface inspection, biomedical, outer space, and extended reality (XR) industries.
USP33 promotes pancreatic cancer malignant phenotype through the regulation of TGFBR2/TGFβ signaling pathway
Authors: Liu Xinyuan , Xu Jian , shen Bingbing , Xu Jichuan , Jiang Jianxin
Published: June 2023

Pancreatic cancer (PC) ranked fourth among cancer-related death worldwide with a survival rate less than 5%. The abnormal proliferation and distant metastasis are major obstacles for the diagnosis and treatment of pancreatic cancer, therefore, it is urgent for researchers to uncover the molecular mechanisms underlying the PC proliferation and metastasis. In current study, we found that USP33, a member of deubiquitinating enzyme family, was upregulated among PC samples and cells, meanwhile, the high expression of USP33 correlated with poor prognosis of patients. Function experiments revealed that USP33 overexpression promoted the proliferation, migration and invasion of PC cells while the inhibition of USP33 expression in PC cells exhibited the opposite effect. The mass spectrum and luciferase complementation assay screened TGFBR2 as the potential binding protein of USP33. Mechanistically, USP33 triggered the deubiquitination of TGFBR2 and prevented its degradation by lysosome, therefore promoted TGFBR2 accumulation in cell membrane and eventually contributed to the sustained activation of TGF-β signaling. Moreover, our results revealed that the activation of TGF-β targeted gene ZEB1 promoted the transcription of USP33. In conclusion, our study found that USP33 contributed to the proliferation and metastasis of pancreatic cancer through a positive feedback loop with TGF-β signaling pathway. Moreover, this study suggested that USP33 may serve as a potential prognostic and therapeutic target in PC.
Comparative phylotranscriptomics reveals ancestral and derived root nodule symbiosis programmes
Authors: Libourel Cyril , Keller Jean , Brichet Lukas , Cazalé Anne-Claire , Carrère Sébastien , Vernié Tatiana , Couzigou Jean-Malo , Callot Caroline , Dufau Isabelle , Cauet Stéphane , Marande William , Bulach Tabatha , Suin Amandine , Masson-Boivin Catherine , Remigi Philippe , Delaux Pierre-Marc , Capela Delphine
Published: June 2023

Symbiotic interactions such as the nitrogen-fixing root nodule symbiosis (RNS) have structured ecosystems during the evolution of life. Here we aimed at reconstructing ancestral and intermediate steps that shaped RNS observed in extant flowering plants. We compared the symbiotic transcriptomic responses of nine host plants, including the mimosoid legume Mimosa pudica for which we assembled a chromosome-level genome. We reconstructed the ancestral RNS transcriptome composed of most known symbiotic genes together with hundreds of novel candidates. Cross-referencing with transcriptomic data in response to experimentally evolved bacterial strains with gradual symbiotic proficiencies, we found the response to bacterial signals, nodule infection, nodule organogenesis and nitrogen fixation to be ancestral. By contrast, the release of symbiosomes was associated with recently evolved genes encoding small proteins in each lineage. We demonstrate that the symbiotic response was mostly in place in the most recent common ancestor of the RNS-forming species more than 90 million years ago.
AAV-mediated expression of a new conformational anti-aggregated α-synuclein antibody prolongs survival in a genetic model of α-synucleinopathies
Authors: Düchs Matthias , Blazevic Dragica , Rechtsteiner Philipp , Kenny Cynthia , Lamla Thorsten , Low Sarah , Savistchenko Jimmy , Neumann Manuela , Melki Ronald , Schönberger Tanja , Stierstorfer Birgit , Wyatt David , Igney Frederik , Ciossek Thomas
Published: June 2023

Prion-like transmission of pathology in α-synucleinopathies like Parkinson’s disease or multiple system atrophy is increasingly recognized as one potential mechanism to address disease progression. Active and passive immunotherapies targeting insoluble, aggregated α-synuclein are already being actively explored in the clinic with mixed outcomes so far. Here, we report the identification of 306C7B3, a highly selective, aggregate-specific α-synuclein antibody with picomolar affinity devoid of binding to the monomeric, physiologic protein. 306C7B3 binding is Ser129-phosphorylation independent and shows high affinity to several different aggregated α-synuclein polymorphs, increasing the likelihood that it can also bind to the pathological seeds assumed to drive disease progression in patients. In support of this, highly selective binding to pathological aggregates in postmortem brains of MSA patients was demonstrated, with no staining in samples from other human neurodegenerative diseases. To achieve CNS exposure of 306C7B3, an adeno-associated virus (AAV) based approach driving expression of the secreted antibody within the brain of (Thy-1)-[A30P]-hα-synuclein mice was used. Widespread central transduction after intrastriatal inoculation was ensured by using the AAV2HBKO serotype, with transduction being spread to areas far away from the inoculation site. Treatment of (Thy-1)-[A30P]-hα-synuclein mice at the age of 12 months demonstrated significantly increased survival, with 306C7B3 concentration reaching 3.9 nM in the cerebrospinal fluid. These results suggest that AAV-mediated expression of 306C7B3, targeting extracellular, presumably disease-propagating aggregates of α-synuclein, has great potential as a disease-modifying therapy for α-synucleinopathies as it ensures CNS exposure of the antibody, thereby mitigating the selective permeability of the blood-brain barrier.
ChAdOx1 nCoV-19 (AZD1222) vaccine-induced Fc receptor binding tracks with differential susceptibility to COVID-19
Authors: Kaplonek Paulina , Cizmeci Deniz , Kwatra Gaurav , Izu Alane , Lee Jessica Shih-Lu , Bertera Harry L. , Fischinger Stephanie , Mann Colin , Amanat Fatima , Wang Wenjun , Koen Anthonet L. , Fairlie Lee , Cutland Clare L. , Ahmed Khatija , Dheda Keertan , Barnabas Shaun L. , Bhorat Qasim Ebrahim , Briner Carmen , Krammer Florian , Saphire Erica Ollman , Gilbert Sarah C. , Lambe Teresa , Pollard Andrew J. , Nunes Marta , Wuhrer Manfred , Lauffenburger Douglas A. , Madhi Shabir A. , Alter Galit
Published: June 2023

Despite the success of COVID-19 vaccines, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern have emerged that can cause breakthrough infections. Although protection against severe disease has been largely preserved, the immunological mediators of protection in humans remain undefined. We performed a substudy on the ChAdOx1 nCoV-19 (AZD1222) vaccinees enrolled in a South African clinical trial. At peak immunogenicity, before infection, no differences were observed in immunoglobulin (Ig)G1-binding antibody titers; however, the vaccine induced different Fc-receptor-binding antibodies across groups. Vaccinees who resisted COVID-19 exclusively mounted FcγR3B-binding antibodies. In contrast, enhanced IgA and IgG3, linked to enriched FcγR2B binding, was observed in individuals who experienced breakthrough. Antibodies unable to bind to FcγR3B led to immune complex clearance and resulted in inflammatory cascades. Differential antibody binding to FcγR3B was linked to Fc-glycosylation differences in SARS-CoV-2-specific antibodies. These data potentially point to specific FcγR3B-mediated antibody functional profiles as critical markers of immunity against COVID-19.
High-density scalp electroencephalogram dataset during sensorimotor rhythm-based brain-computer interfacing
Authors: Iwama Seitaro , Morishige Masumi , Kodama Midori , Takahashi Yoshikazu , Hirose Ryotaro , Ushiba Junichi
Published: June 2023

Real-time functional imaging of human neural activity and its closed-loop feedback enable voluntary control of targeted brain regions. In particular, a brain-computer interface (BCI), a direct bridge of neural activities and machine actuation is one promising clinical application of neurofeedback. Although a variety of studies reported successful self-regulation of motor cortical activities probed by scalp electroencephalogram (EEG), it remains unclear how neurophysiological, experimental conditions or BCI designs influence variability in BCI learning. Here, we provide the EEG data during using BCIs based on sensorimotor rhythm (SMR), consisting of 4 separate datasets. All EEG data were acquired with a high-density scalp EEG setup containing 128 channels covering the whole head. All participants were instructed to perform motor imagery of right-hand movement as the strategy to control BCIs based on the task-related power attenuation of SMR magnitude, that is event-related desynchronization. This dataset would allow researchers to explore the potential source of variability in BCI learning efficiency and facilitate follow-up studies to test the explicit hypotheses explored by the dataset.
Spatio-temporal evolution and influencing factors of China’s ICT service industry
Authors: Chen Weixuan , Zhang Shiwei , Kong Dezhou , Zou Tong , Zhang Yuxi , Cheshmehzangi Ali
Published: June 2023

The ICT service industry has become a burgeoning industry at a high and stable speed. Their equitable distribution can improve national and global positive peace. This paper aimed to verify the characteristics of spatio-temporal evolution and its influencing factors in the ICT service industry. Based on the data from 31 Provinces in China from 2015 to 2019, this paper uses location quotient, spatial autocorrelation methods and spatial econometric analysis to explore the development characteristics, evolution and influencing factors of the ICT service industry, respectively. The main results are shown as follows: (1) China's ICT service industry is mainly concentrated in Beijing, Shanghai, Zhejiang, Tibet, and Guangdong, with a trend of specialisation development. They are not only distributed in cities with relatively superior overall development but also those with superior industrial and development carrier elements. Technological relevance, aggregation, and political difference might have an impact on promoting the emergence and development of these industries. (2) ICT service industry is characterised by stable and highly concentrated development. Numbers between three to five significant provinces and types with high-high (HH) and high-low (HL) clusters of local spatio-temporal association kept stable in the period. The HH was in eastern coastal areas, including Zhejiang, Shanghai, Jiangsu, and Shandong, and the HL was in Guangdong in 2015. There is a definite spatial correlation in spatial distribution with constant strengthening. (3) TUR, NDN, MIAT and the area were shown to have a significant role in promoting the ICT service industry, while NW, GDP and ICT Employment were shown to have a significant negative impact on this industry. Correspondingly, two strategies were put forward here: (1) accelerating the inter-provincial networking development of the ICT service industry, and (2) strengthening government policy guidance for the ICT service industry. These outcomes can not only provide a scientific basis and theoretical support for the distribution of strategies and resources for these industries at the theoretical level but also improve resource integration from the national perspective and the efficiency of resource use at the practical level.
The risk of being bitten by a dog is higher on hot, sunny, and smoggy days
Authors: Dey Tanujit , Zanobetti Antonella , Linnman Clas
Published: June 2023

Humans commit more violent crimes when temperature and air pollution is higher. Here, we investigate if also the day-to-day rates of dogs biting humans is influenced by environmental factors. 69,525 reports of dogs biting humans, sourced from public records on animal control requests and from ER records, were analyzed. The impact of temperature and air pollutants were evaluated with a zero-inflated Poisson generalized additive model, while controlling for regional and calendar effects. Exposure–response curves were used to assess the association between outcome and major exposure variables. We find that the rates of dogs biting humans increases with increasing temperature and ozone, but not PM2.5 exposure. We also observed that higher UV irradiation levels were related to higher rats of dog bites. We conclude that dogs, or the interactions between humans and dogs, are more hostile on hot, sunny, and smoggy days, indicating that the societal burden of extreme heat and air pollution also includes the costs of animal aggression.
Facial mimicry and metacognitive judgments in emotion recognition are distinctly modulated by social anxiety and autistic traits
Authors: Folz Julia , Akdağ Rüya , Nikolić Milica , van Steenbergen Henk , Kret Mariska E.
Published: June 2023

Facial mimicry as well as the accurate assessment of one's performance when judging others’ emotional expressions have been suggested to inform successful emotion recognition. Differences in the integration of these two information sources might explain alterations in the perception of others’ emotions in individuals with Social Anxiety Disorder and individuals on the autism spectrum. Using a non-clinical sample (N = 57), we examined the role of social anxiety and autistic traits in the link between facial mimicry, or confidence in one’s performance, and emotion recognition. While participants were presented with videos of spontaneous emotional facial expressions, we measured their facial muscle activity, asked them to label the expressions and indicate their confidence in accurately labelling the expressions. Our results showed that confidence in emotion recognition was lower with higher social anxiety traits even though actual recognition was not related to social anxiety traits. Higher autistic traits, in contrast, were associated with worse recognition, and a weakened link between facial mimicry and performance. Consequently, high social anxiety traits might not affect emotion recognition itself, but the top-down evaluation of own abilities in emotion recognition contexts. High autistic traits, in contrast, may be related to lower integration of sensorimotor simulations, which promote emotion recognition.
Disparities in insecurity, social support, and family relationships in association with poor mental health among US adults during the COVID-19 pandemic
Authors: Zhu Kexin , Wang Siyi , Yue Yihua , Smith Beth A. , Zhang Zuo-Feng , Freudenheim Jo L. , Niu Zhongzheng , Zhang Joanne , Smith Ella , Ye Joshua , Cao Ying , Zhang Jie , Hennessy Dwight A. , Lei Lijian , Mu Lina
Published: June 2023

The COVID-19 pandemic has had a significant impact on mental health. Identifying risk factors and susceptible subgroups will guide efforts to address mental health concerns during the pandemic and long-term management and monitoring after the pandemic. We aimed to examine associations of insecurity (concerns about food, health insurance, and/or money), social support, and change in family relationships with poor mental health and to explore disparities in these associations. An online survey was collected from 3952 US adults between May and August 2020. Symptoms of anxiety, depression, stress, and trauma-related disorders were assessed by the Generalized Anxiety Disorder 7-item scale, the Patient Health Questionnaire-9, the Perceived Stress Scale-4, and the Primary Care Post-Traumatic Stress Disorder Screen, respectively. Social support was measured by the Oslo Social Support Scale. Logistic regression was used and stratified analyses by age, race/ethnicity, and sex were performed. We found a higher prevalence of poor mental health among those who were younger, female, with lower socioeconomic status, and racial/ethnic minorities. Participants who were worried about money, health insurance, or food had higher odds of symptoms of anxiety (OR = 3.74, 95% CI: 3.06–4.56), depression (OR = 3.20, 95% CI: 2.67–3.84), stress (OR = 3.08, 95% CI: 2.67–3.57), and trauma-related disorders (OR = 2.93, 95% CI: 2.42–3.55) compared to those who were not. Compared to poor social support, moderate and strong social support was associated with lower odds of all four symptoms. Participants who had changes in relationships with parents, children, or significant others had worse mental health. Our findings identified groups at higher risk for poor mental health, which offers insights for implementing targeted interventions.
Prosopis juliflora hydrothermal synthesis of high fluorescent carbon dots and its antibacterial and bioimaging applications
Authors: Prathap Nadarajan , Balla Putrakumar , Shivakumar Muthugoundar Subramanian , Periyasami Govindasami , Karuppiah Ponmurugan , Ramasamy Krishnaraj , Venkatesan Srinivasan
Published: June 2023

Carbon dots have stimulated the curiosity of biomedical researchers due to their unique properties, such as less toxicity and high biocompatibility. The synthesis of carbon dots for biomedical application is a core area in research. In the current research, an eco-friendly hydrothermal technique was employed to synthesize high fluorescent, plant-derived carbon dots from Prosopis juliflora leaves extract (PJ-CDs). The synthesized PJ-CDs were investigated by physicochemical evaluation instruments such as fluorescence spectroscopy, SEM, HR-TEM, EDX, XRD, FTIR, and UV-Vis. The UV-Vis absorption peaks obtained at 270 nm due to carbonyl functional groups shifts of n→π*. In addition, a quantum yield of 7.88 % is achieved. The synthesized PJ-CDs showing the presence of carious functional groups O–H, C–H, C=O, O–H, C–N and the obtained particles in spherical shape with an average size of 8 nm. The fluorescence PJ-CDs showed stability against various environmental factors such as a broad range of ionic strength and pH gradient. The antimicrobial activity of PJ-CDs was tested against a Staphylococcus aureus, and a Escherichia coli. The results suggest that the PJ-CDs could substantially inhibit the growth of Staphylococcus aureus. The findings also indicate that PJ-CDs are effective materials for bio-imaging in Caenorhabditis elegans and they can be also used for pharmaceutical applications.
Slow and population specific evolutionary response to a warming environment
Authors: Santos Marta A. , Antunes Marta A. , Grandela Afonso , Quina Ana S. , Santos Mauro , Matos Margarida , Simões Pedro
Published: June 2023

Adaptation to increasingly warmer environments may be critical to avoid extinction. Whether and how these adaptive responses can arise is under debate. Though several studies have tackled evolutionary responses under different thermal selective regimes, very few have specifically addressed the underlying patterns of thermal adaptation under scenarios of progressive warming conditions. Also, considering how much past history affects such evolutionary response is critical. Here, we report a long-term experimental evolution study addressing the adaptive response ofDrosophila subobscurapopulations with distinct biogeographical history to two thermal regimes. Our results showed clear differences between the historically differentiated populations, with adaptation to the warming conditions only evident in the low latitude populations. Furthermore, this adaptation was only detected after more than 30 generations of thermal evolution. Our findings show some evolutionary potential ofDrosophilapopulations to respond to a warming environment, but the response was slow and population specific, emphasizing limitations to the ability of ectotherms to adapt to rapid thermal shifts.
Compact conformal tattoo-polymer antenna for on-body wireless power transfer
Authors: Chang Xi Liang , Chee Pei Song , Lim Eng Hock
Published: June 2023

This paper presents a 35.0 × 35.0 × 2.7 mm3 compact, low-profile, and lightweight wearable antenna for on-body wireless power transfer. The proposed antenna can be easily printed on a piece of flexible tattoo paper and transformed onto a PDMS substrate, making the entire antenna structure conform to the human body for achieving a better user experience. Here, a layer of frequency selective surface (FSS) is inserted in between the antenna and human tissue, which has successfully reduced the loading effects of the tissue, with 13.8 dB improvement on the antenna gain. Also, the operating frequency of the rectenna is not affected much by deformation. To maximize the RF-DC conversion efficiency, a matching loop, a matching stub, and two coupled lines are integrated with the antenna for tuning the rectenna so that a wide bandwidth (~ 24%) can be achieved without the use of any external matching networks. Measurement results show that the proposed rectenna can achieve a maximum conversion efficiency of 59.0% with an input power of 5.75 μW/cm2 and can even exceed 40% for a low input power of 1.0 μW/cm2 with a 20 kΩ resistive load, while many other reported rectennas can only achieve a high PCE at a high power density level, which is not always practical for a wearable antenna.