bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy of acupuncture for functional constipation in elderly: a systematic review and meta-analysis
Authors: Song ShiWei , Hao WenFeng , Fu HongFang
Published: December 2024

Background

Numerous clinical studies have shown that patients suffering from functional constipation can benefit by combining medication with acupuncture. There have been no published reviews or meta-analyses regarding the use of acupuncture in treating functional constipation in older adults. Therefore, we carried out a meta-analysis to assess the impact of acupuncture on elderly patients dealing with functional constipation.

Methods

This study retrieved randomized controlled trials (RCTs) on acupuncture therapy for functional constipation in the elderly from several electronic databases, including Embase, PubMed, Cochrane Library, Wanfang Database, Chinese BioMedical Literature Database, and China National Knowledge Infrastructure. In these databases, clinical investigators evaluated the effectiveness of acupuncture as a primary treatment for elderly people with functional constipation. The Cochrane Handbook for Systematic Reviews of Interventions was used by researchers to evaluate the quality of the study.

Results

A total of 469 elderly individuals were included in 8 RCTs. The meta-analysis yielded compelling findings: the application of acupuncture has significantly elevated the rates of treatment effectiveness compared to the control group. Compared with the control group, the treatment group exhibited a statistically significant difference in complete spontaneous bowel movements after treatment. The two groups showed no significant difference in spontaneous bowel movements. However, there was a significant difference in the Bristol Stool Scale scores. The Defecation Difficulty Score and Patient Assessment of Constipation Quality of Life (PAC-QOL) showed p-values that indicated no significant effect. However, acupuncture improved bowel movements, demonstrating a significant difference in the Clinic Constipation Score (CCS) when comparing the two groups. The Nitric Oxide Synthase (NOS) and 5-Hydroxytryptamine (5-HT) contents changed significantly after intervention in both groups. An article reported that the improvement of traditional Chinese medicine (TCM) symptom scores was better in the treatment group than in the control group.

Conclusion

The analysis results indicated that acupuncture can be beneficial for elderly people with functional constipation; however, strong and comprehensive data are not yet obtainable. Given that our study is based on evidence that is of a low-to-moderate quality, further high-quality research is necessary to enhance the feasibility and practicability of this treatment.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42024533215.
A slow-release strategy of Lactobacillus plantarum to enhance the degradation of cellulose by Bacillus methylotrophic in the ensiling process of corn stalk
Authors: Wan Yongqing , Liu Siyuan , Song Yunhao , Tian Ruihua , Wang Ruigang , Duan Kaihong , Mandlaa Mandlaa
Published: December 2024

The enhancement of cellulose degradation is important for improving the quality of corn-stalk silage. However, the rapid drop in pH caused by the propagation of lactic acid bacteria (LAB) can influence the degradation of cellulose by cellulose-degrading microorganisms (CDMs) during the mixed fermentation process of ensilage. In this study, a CDM (Bacillus methylotrophic 2–4, BM 2–4) was isolated, and its lyophilization condition was studied. Moreover, a slow-release strategy was developed to delay the release of LAB (Lactobacillus plantarum S-1, LP S-1) by embedding technology to provide time for BM 2–4 to degrade cellulose during the corn-stalk ensilage process. The results showed that BM 2–4 had a higher survival rate (89.53%) under the following conditions: cell collection (5,600 r/min in 4.4°C for 9.5-min centrifugation) and lyophilization using cryoprotectants [skim milk (10.4%), peptone (10.4%), and glucose (5.2%)] at −50°C with a vacuum pressure of <10 Pa. Based on the results of the previous study on embedded LP S-1, simultaneous inoculation of embedded LP S-1 and lyophilized BM2-4 at a 2:1 ratio, with an inoculum size of 6% and moisture content of 85%, significantly degraded CF by 3.8% and increased CP by 3.7% (p < 0.05). This treatment did not significantly influence the final pH of corn-stalk silage (p > 0.05) after 7 days of fermentation.
Amelioration of inflammatory bowel disease by Bifidobacterium animalis subsp. lactis XLTG11 in combination with mesalazine
Authors: Ma Weiwei , Wu Yanan , Lin Xinyue , Yang Liping , Huang Lili
Published: December 2024

The treatment of inflammatory bowel disease (IBD) remains challenging and significantly impacts both patients and their families. This study evaluated the role of Bifidobacterium animalis subsp. lacti XLTG11 (XLTG11) in combination with mesalazine (5-ASA) in the improvement of IBD. The results demonstrated that the XLTG11+5-ASA group exhibited superior recovery compared to both the XLTG11-only group and the 5-ASA-only group. The XLTG11+5-ASA group significantly reduced myeloperoxidase activity (MPO), attenuated colonic tissue damage, lowered the levels of lipopolysaccharides (LPS) and D-lactic acid (D-LA), and decreased intestinal permeability. Furthermore, it upregulated the mRNA expression of Claudin-1, Occludin, ZO-1, and MUC2, which contributed to the protective effect on intestinal barrier function. Additionally, the XLTG11+5-ASA group significantly increased the levels of anti-inflammatory cytokines while decreasing pro-inflammatory cytokine levels. Notably, treatment with the XLTG11+5-ASA group significantly increased levels of acetic, propionic, and butyric acids, as well as the relative abundance of beneficial bacteria such as Bifidobacterium and Lactobacillus, while decreasing the relative abundance of Enterococcus, Enterobacteriaceae, and Clostridium perfringens. The results indicate that the combination of XLTG11 and 5-ASA was more effective in treating IBD than either treatment alone, significantly improving IBD-related symptoms and providing a scientific basis for future clinical applications.
Symbiotic microbial population composition of Apolygus lucorum under temperature and pesticide pressures
Authors: Ma Mengxin , Xue Hui , Zhu Xiangzhen , Wang Li , Niu Lin , Luo Junyu , Cui Jinjie , Gao Xueke
Published: December 2024

Insect population control using pesticides faces new challenges as global temperatures change. Symbiotic bacteria of insects play a key role in insect resistance to pesticides, and these symbiotic bacteria themselves are sensitive to the effects of temperature changes. Apolygus lucorum, a sucking pest, survives in a wide range of temperatures (15°C–35°C), and is presently controlled predominantly using the pesticide imidacloprid. Here, we investigated the effects of temperature and imidacloprid on A. lucorum microbial population composition using 16S rRNA sequencing. We found that the application of imidacloprid in high-temperature environments led to an increase in the species diversity of bacteria in the body of A. lucorum. High temperatures may disrupt the symbiotic relationship between certain bacteria and A. lucorum, such as Cedecea neteri. High temperatures led to a decrease in the abundance of Cedecea neteri. Agathobaculum butyriciproducens, Advenella migardefenensis, and Akkermansia muciniphila were very sensitive to temperature and were strongly affected by temperature changes. Microorganisms that were greatly affected by the concentration of imidacloprid in the community include Aeromonas caviae and Akkermansia muciniphila. The aim of this study is to reveal the dynamics and diversity of symbiotic bacteria of A. lucorum treated with imidacloprid at a range of temperatures. These results provide insight into new strategies for pest control in a changing climate.
Molecular surveillance and antimicrobial susceptibility profile of bacterial contamination in pastries of Iranian confectioneries: a public health concern
Authors: Hosseini Shiva , Motallebirad Tahereh , Mohammadi Mohammad Reza , Safarabadi Mehdi , Beheshti Zeynab , Orouji Mohammad Ali , Mardanshah Omid , Azadi Davood
Published: December 2024

Introduction

Microbial contamination in food products such as pastries, poses a significant public health concern due to the potential risks of foodborne infection and outbreak, Therefore, to prevent these infections, it is essential to investigate the frequency and extent of microbial contamination as well as the level of drug resistance in pastries. Due to this issue, our study aimed to assess the microbial diversity and the drug susceptibility patterns of microbial pollutants in pastry shops in Markazi province, Iran.

Methods

The study involved collecting 120 pastry samples from 30 pastry shops in Markazi province, Iran. The isolates were identified using a series of biochemical, phenotypic, and molecular assays, including specific PCR and 16S rRNA gene sequencing. Drug susceptibility testing (AST) was performed by using the Kirby-Bauer method according to the CLSI 2023 guidelines.

Results and discussion

A total of 56 isolates (46.66%) were recovered from 120 pastries samples, The most prevalent species isolated in the current study were S. aureus 12 isolates (21.43%), M. luteus 7 isolates (12.5%), E. coli 7 isolates (12.5%), S. warneri 6 isolates (11.12%), 6 isolates of S. succinus (11.12%), B. cereus 5 isolates (10.7%), Nocardia 4 isolates (7.15%), K. pneumoniae 3 isolates (5.35%), S. epidermidis 3 isolates (5.35%), and E. faecium 3 isolates (5.35%). The isolates showed the most sensitivity to imipenem and trimethoprim-sulfamethoxazole and the least sensitivity to erythromycin and tetracycline. The AST showed that 7 isolates of S. aureus were MRSA, 3 isolates of E. coli and, 2 isolates of K. pneumoniae were identified as ESBL. In conclusion, the results of the current study showed that the microbial contamination of pastries produced in confectionaries of Markazi province was not in standard ranges. These problems may be related to fecal contamination of pastries or lack of hygiene by handlers and it is urgent to develop the standards of hygiene of food handling techniques and aseptic pastry production in confectioneries.
Relationship between the risk of intestinal mucosal Epstein–Barr virus and/or cytomegalovirus infection and peripheral blood NK cells numbers in patients with ulcerative colitis: a cross-sectional study in Chinese population
Authors: Tian Ye , Dai Jinghua , Yang Yunfeng , Guo Xiaofeng , Wang Wei , Li Fengxia , Wang Juzi , Liu Ruiyun
Published: December 2024

Objective

This study aimed to analyze the relationship between the risk of common opportunistic pathogens Epstein–Barr virus (EBV) and cytomegalovirus (CMV) infection in intestinal mucosal tissues of Ulcerative Colitis (UC) patients and the number of peripheral blood NK cells.

Methods

UC patients admitted to a third-grade class-A hospital from January 2018 to December 2023 were selected as research population. Clinical data of the patients were collected from the electronic medical record system. Additionally, samples of intestinal mucosal tissues were obtained for real-time fluorescence quantitative PCR to detect and analyze the viral load of CMV and EBV. Blood samples were collected for lymphocyte subsets analysis. Multivariable logistic regression models analyses was used to determine the odds ratio (OR) and 95% confidence interval (95% CI) for the independent association between NK cells and EBV/CMV infections in UC. We further applied the restricted cubic spline analysis and smooth curve fitting to examine the non-linear relationship between them.

Results

378 UC patients were enrolled. Of these patients, there were 194 patients (51.32%) with EBV /CMV infection. In multivariable logistic regression analyses NK cells was independently associated with EBV and/or CMV infection after adjusted potential confounders (OR 8.24, 95% CI 3.75–18.13, p < 0.001). A nonlinear relationship was found between NK cells and EBV/CMV infections, which had a threshold around 10.169. The effect sizes and CIs below and above the threshold were 0.535 (0.413–0.692), p < 0.001 and 1.034 (0.904–1.183), p > 0.05, respectively.

Conclusion

There was a non-linear relationship between NK cells and EBV/CMV infections. The risk for EBV/CMV infections was not increased with increasing NK cells in individuals with NK cells ≥ 10.169%, whereas the risk for EBV and/or CMV infection was increased with an decreasing NK cells in those with NK cells < 10.169%. The risk of EBV/CMV infections increases when NK cells were below a certain level.
Posture-invariant myoelectric control with self-calibrating random forests
Authors: Jiang Xinyu , Ma Chenfei , Nazarpour Kianoush
Published: December 2024

Introduction

Myoelectric control systems translate different patterns of electromyographic (EMG) signals into the control commands of diverse human-machine interfaces via hand gesture recognition, enabling intuitive control of prosthesis and immersive interactions in the metaverse. The effect of arm position is a confounding factor leading to the variability of EMG characteristics. Developing a model with its characteristics and performance invariant across postures, could largely promote the translation of myoelectric control into real world practice.

Methods

Here we propose a self-calibrating random forest (RF) model which can (1) be pre-trained on data from many users, then one-shot calibrated on a new user and (2) self-calibrate in an unsupervised and autonomous way to adapt to varying arm positions.

Results

Analyses on data from 86 participants (66 for pre-training and 20 in real-time evaluation experiments) demonstrate the high generalisability of the proposed RF architecture to varying arm positions.

Discussion

Our work promotes the use of simple, explainable, efficient and parallelisable model for posture-invariant myoelectric control.
An improved graph factorization machine based on solving unbalanced game perception
Authors: Xie Xiaoxia , Jia Yuan , Ma Tiande
Published: December 2024

The user perception of mobile game is crucial for improving user experience and thus enhancing game profitability. The sparse data captured in the game can lead to sporadic performance of the model. This paper proposes a new method, the balanced graph factorization machine (BGFM), based on existing algorithms, considering the data imbalance and important high-dimensional features. The data categories are first balanced by Borderline-SMOTE oversampling, and then features are represented naturally in a graph-structured way. The highlight is that the BGFM contains interaction mechanisms for aggregating beneficial features. The results are represented as edges in the graph. Next, BGFM combines factorization machine (FM) and graph neural network strategies to concatenate any sequential feature interactions of features in the graph with an attention mechanism that assigns inter-feature weights. Experiments were conducted on the collected game perception dataset. The performance of proposed BGFM was compared with eight state-of-the-art models, significantly surpassing all of them by AUC, precision, recall, and F-measure indices.
Investigating the acute cognitive effects of dietary compounds using fNIRS: methodological limitations and perspectives for research targeting healthy adults
Authors: Zahar Sélima , Van de Ville Dimitri , Hudry Julie
Published: December 2024

The brain’s response to cognitive demands hinges on sufficient blood flow, with changes in brain hemodynamics serving as a reflection of this process. Certain bioactive compounds found in our diet, such as caffeine, polyphenols, and nitrate, can acutely impact brain hemodynamics through diverse neural, vasoactive, and metabolic mechanisms. Functional Near-Infrared Spectroscopy (fNIRS) offers a non-invasive and real-time method to investigate these effects. Despite their potential, fNIRS studies investigating the acute impacts of bioactive compounds on cognition face methodological gaps, especially in controlling confounding factors. Given the impact of these confounding effects, which can be significant due to the relatively limited sample size of such studies, there is a need to refine the methodologies employed. This review proposes recommendations to enhance current methodologies in the research field, focusing on key aspects of the data collection phase, including research design, experimental paradigms, and participant demographics, and their integration into the analysis phase. Ultimately, it seeks to advance our understanding of the effects of bioactive compounds on cognitive functions to contribute to the development of targeted nutritional interventions for improved brain health.
Transcutaneous auricular vagus nerve stimulation as a potential therapy for attention deficit hyperactivity disorder: modulation of the noradrenergic pathway in the prefrontal lobe
Authors: Zhi Jincao , Zhang Shiwen , Huang Meiling , Qin Huan , Xu He , Chang Qing , Wang Yan
Published: December 2024

Attention deficit hyperactivity disorder (ADHD) is a neurodevelopmental disorder characterized by developmental impairments, inattention, motor hyperactivity, and impulsivity. Currently, there is no effective intervention that can completely cure it. One of the pathogenic mechanisms of ADHD involves abnormalities in the norepinephrine (NE) pathway within the prefrontal cortex (PFC). In recent years, transcutaneous auricular vagus nerve stimulation (taVNS), a non-invasive neuromodulation technique, has demonstrated promising potential in the treatment of neurological and psychiatric disorders. However, its application in the management of ADHD remains relatively unexplored. Previous studies have shown that taVNS exerts therapeutic effects on attention, cognition, arousal, perception, and behavioral regulation primarily through activating the vagus nerve conduction pathway, specifically targeting the nucleus tractus solitarius - locus coeruleus - NE pathway. These findings have led to the hypothesis that taVNS may be an effective intervention for ADHD, with NE and its pathway playing a pivotal role in this context. Therefore, this review comprehensively examines the correlation between NE pathway alterations in the PFC and ADHD, the mechanism of action of taVNS, and the potential role of the NE pathway in treating ADHD with taVNS, aiming to provide a theoretical foundation for clinical applications.
Differential effects of isoflurane on auditory and visually evoked potentials in the cat
Authors: Bao Xiaohan , Barnes Paisley , Lomber Stephen G.
Published: December 2024

Evoked potentials can be used as an intraoperative monitoring measure in neurological surgery. Auditory evoked potentials (AEPs), or specifically brainstem auditory evoked responses (BAERs), are known for being minimally affected by anesthetics, while visually evoked potentials (VEPs) are presumed to be unreliable and easily affected by anesthetics. While many anesthesia trials or intraoperative recordings have provided evidence in support of these hypotheses, the comparisons were always made between AEPs and VEPs recorded sequentially, rather than recorded at the same time. Although the logistics of improving data comparability of AEPs and VEPs may be a challenge in clinical settings, it is much more approachable in animal models to measure AEPs and VEPs as simultaneously as possible. Five cats under dexmedetomidine sedation received five, 10-min blocks of isoflurane with varying concentrations while click-evoked AEPs and flash-evoked VEPs were recorded from subdermal electrodes. We found that, in terms of their waveforms, (1) short-latency AEPs (BAERs) were the least affected while middle-latency AEPs were dramatically altered by isoflurane, and (2) short-latency VEPs was less persistent than that of short-latency AEPs, while both middle- and long-latency VEPs were largely suppressed by isoflurane and, in some cases, completely diminished. In addition, the signal strength in all but the middle-latency AEPs was significantly suppressed by isoflurane. We identified multiple AEP or VEP peak components demonstrating suppressed amplitudes and/or changed latencies by isoflurane. Overall, we confirmed that both cat AEPs and VEPs are affected during isoflurane anesthesia, as in humans.
Fructose-mediated AGE-RAGE axis: approaches for mild modulation
Authors: Semchyshyn Halyna
Published: December 2024

Fructose is a valuable and healthy nutrient when consumed at normal levels (≤50 g/day). However, long-term consumption of excessive fructose and elevated endogenous production can have detrimental health impacts. Fructose-initiated nonenzymatic glycation (fructation) is considered as one of the most likely mechanisms leading to the generation of reactive species and the propagation of nonenzymatic processes. In the later stages of glycation, poorly degraded advanced glycation products (AGEs) are irreversibly produced and accumulated in the organism in an age- and disease-dependent manner. Fructose, along with various glycation products—especially AGEs—are present in relatively high concentrations in our daily diet. Both endogenous and exogenous AGEs exhibit a wide range of biological effects, mechanisms of which can be associated with following: (1) AGEs are efficient sources of reactive species in vivo, and therefore can propagate nonenzymatic vicious cycles and amplify glycation; and (2) AGEs contribute to upregulation of the specific receptor for AGEs (RAGE), amplifying RAGE-mediated signaling related to inflammation, metabolic disorders, chronic diseases, and aging. Therefore, downregulation of the AGE-RAGE axis appears to be a promising approach for attenuating disease conditions associated with RAGE-mediated inflammation. Importantly, RAGE is not specific only to AGEs; it can bind multiple ligands, initiating a complex RAGE signaling network that is not fully understood. Maintaining an appropriate balance between various RAGE isoforms with different functions is also crucial. In this context, mild approaches related to lifestyle—such as diet optimization, consuming functional foods, intake of probiotics, and regular moderate physical activity—are valuable due to their beneficial effects and their ability to mildly modulate the fructose-mediated AGE-RAGE axis.
Signal detection and analysis of adverse events associated with Genvoya® based the FAERS database
Authors: Wang Chengliang , Zhang Yan , Tang Xiting , Zhang Guoping , Chen Li
Published: December 2024

Objective

This study aims to evaluate and understand the safety profile of Genvoya® by mining and analyzing adverse drug event (ADE, adverse drug event) reports from the FDA Adverse Event Reporting System (FAERS, FDA Adverse Event Reporting System) database, thus providing valuable reference information for individuals infected with HIV.

Methods

Data were obtained from the FAERS database, covering the period from the first quarter of 2015 to the fourth quarter of 2023, focusing on reports where Genvoya® was the primary suspected drug. Data import and extraction were conducted using MySQL 8.0, with adverse events standardized according to the Medical Dictionary for Regulatory Activities (MedDRA, Medical Dictionary for Regulatory Activities) 27.0 terminology. Potential adverse event signals were identified through disproportionality analysis, including the reporting odds ratio (ROR, reporting odds ratio) method and the comprehensive standard by the Medicines and Healthcare products Regulatory Agency (MHRA, Medicines and Healthcare products Regulatory Agency) method. Statistical analyses and graphical representations were performed.

Results

A total of 2, 376 adverse drug event reports related to Genvoya® were analyzed. Reports from male patients accounted for 74.33%, while those from female patients accounted for 22.39%. Common adverse events included weight gain, drug interactions, and increased viral load. Additionally, new potential adverse reactions, such as fat redistribution, HIV-associated neurocognitive disorders, and meningoencephalitis, were identified. These reactions were not adequately described in the existing literature and drug labels.

Conclusion

Multiple adverse reactions were observed with the use of Genvoya®. Clinicians should closely monitor these reactions and implement necessary preventive and intervention measures based on patient-specific conditions and treatment guidelines. Although this study has limitations, the analysis of FAERS database data has revealed various potential risks associated with Genvoya®, providing important safety references for HIV treatment.
Development of a UPLC-MS/MS method for the determination of sulfatinib and its no interaction with myricetin in rats
Authors: Chen Dongxin , Chen Jie , Xia Hailun , Chen Xiaohai , Hu Jinyu , Wu Guangliang , Xu Xuegu
Published: December 2024

Introduction

Sulfatinib is a novel oral tyrosine kinase inhibitor (TKI) with selective inhibition of fibroblast growth factor (FGFR), colony-stimulating factor 1 receptor (CSF-1R) and vascular endothelial growth factor receptor (VEGFR) 1, 2, and 3. It has been approved for the therapy of neuroendocrine tumors arising in the non-pancreatic (December 2020) and pancreatic (June 2021) glands. Until now, there has no research on the determination of sulfatinib in biological medium by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method.

Methods

The current study validated a sensitive and reliable quantitative detection of sulfatinib in plasma using UPLC-MS/MS for the first time, and investigated the interaction with myricetin in rats. Acetonitrile was used to precipitate the plasma protein, and lenvatinib was employed as the internal standard (IS).

Results

The method demonstrated that sulfatinib presented high linearity over the concentration of 11–2,000 ng/mL with the lower limit of quantification (LLOQ) of 1 ng/mL. It was validated methodologically that the precision, matrix effect, stability, accuracy and extraction recovery were all within the allowable values. Moreover, male Sprague-Dawley (SD) rats were assigned randomly to assess the interaction between sulfatinib (30 mg/kg) and myricetin (50 mg/kg). Nevertheless, no significant differences of the main pharmacokinetic parameters were revealed. This may be due to insufficient doses of myricetin, or failure of myricetin to act in a timely manner in vivo.

Discussion

The findings contributed to a better understanding of the metabolism and drug-drug interaction of sulfatinib, but the presence or absence of interactions needs to be confirmed by further studies.
Asparagus officinalis L. extract exhibits anti-proliferative and anti-invasive effects in endometrial cancer cells and a transgenic mouse model of endometrial cancer
Authors: Fang Ziwei , Kong Weimin , Zhao Ziyi , Sun Wenchuan , Xu Guangxu , Clark Leslie H. , Sullivan Stephanie A. , Tran Arthur-Quan , Zhou Chang-Sheng , Sun Delin , Zhao Luyu , Wang Jiandong , Zhou Chunxiao , Bae-Jump Victoria L.
Published: December 2024

Introduction

Endometrial cancer is the most common malignancy of the female reproductive system in the United States. Asparagus officinalis is a versatile, nutrient-dense, low-calorie vegetable that contains various bioactive metabolites that have shown a variety of biologic functions beneficial to health. The metabolites from asparagus officinalis extracts or asparagus officinalis extracts exhibit significant anti-tumorigenic activity in some pre-clinical models of cancer.

Methods

Endometrial cancer cells were used to study the effects of asparagus officinalis on anti-proliferation, anti-invasion and increased sensitivity to cisplatin, and obese and lean Lkb1fl/flp53fl/fl mouse model of endometrial cancer was used to study the role of asparagus officinalis in tumor growth.

Results

Treatment with increasing concentrations of Asparagus officinalis extracts significantly inhibited cell proliferation, reduced glycolytic activity, induced cellular stress and apoptosis, caused cell cycle G1 arrest, increased the sensitivity of cells to cisplatin, reduced cell adhesion and invasion, and activation of AMPK and inhibition of the AKT/mTOR and MAPK signaling pathways in endometrial cancer cells. Moreover, asparagus officinalis extracts suppressed cell adhesion and invasion through the modulation of the epithelial-to-mesenchymal transition process. Asparagus officinalis extract treatment for 4 weeks resulted in a significant reduction in tumor growth in Lkb1fl/flp53fl/fl mice under both obese and lean conditions, with a decrease in Ki-67 and vascular endothelial growth factor expression and an increase in Bip expression in endometrial tumors.

Discussion

These findings provide strong preclinical evidence for the potential therapeutic benefit of asparagus officinalis extract as a novel dietary strategy in the treatment of endometrial cancer. Further clinical trials of dietary intervention of asparagus officinalis or combination with cisplatin in patients with endometrial cancer are warranted.
MADRS single items differential changes among patients with melancholic and unspecified depression treated with ECT: an exploratory study
Authors: Pozuelo Moyano Beatriz , Ranjbar Setareh , Swierkosz-Lenart Kevin , Schuster Jean Pierre , Zullo Leonardo , von Gunten Armin , Vandel Pierre
Published: December 2024

Introduction

Major depressive disorder (MDD) exhibits heterogeneity in treatment response.

Objective

This exploratory analysis aims to evaluate the differential changes in individual items of the MADRS between melancholic MDD (M-MDD) and unspecified MDD (U-MDD) following electroconvulsive therapy (ECT).

Methods

The study included 23 patients with unipolar MDD who received ECT. Patients were classified as M-MDD or U-MDD according to DSM-5 criteria. MADRS scores were assessed at baseline and one-month post-ECT. Differences between subtypes were analyzed using the Wilcoxon test and multiple linear regression.

Results

Among 23 participants receiving ECT for MDD, 10 had M-MDD and 13 had U-MDD. Baseline MADRS items showed significantly higher scores in the M-MDD group, except for reported sadness, suicidal ideation, and concentration difficulties. Total MADRS score reduction was significantly greater in the M-MDD group. This decline was especially pronounced in M-MDD patients for specific items, including apparent sadness, inability to feel, pessimistic thoughts, sleep disturbances, reduced appetite, and concentration difficulties, after adjusting for age and sex.

Conclusion

MADRS score reductions were more substantial for M-MDD than U-MDD in both total and specific items following one month of ECT. Further research with larger samples is needed to clarify MADRS response differences after ECT between melancholic and unspecified depressive subtypes.
Exploring the utilization of targeted intervention services by transgender individuals in Uttarakhand, India: a qualitative study
Authors: Khapre Meenakshi , Sahoo Krushna , Saxena Vartika , Sinha Smita , Luthra Gagandeep , Joshi Anubhuti
Published: December 2024

Purpose

Transgender (TG) women face violence, discrimination, and stigma, which affect their mental health and hinder their access to targeted intervention (TI) services. This lack of access may increase the risk of human immunodeficiency virus/sexually transmitted infections (HIV/STIs). However, the utilization of TI services among transgender women in Uttarakhand, as well as across the country, remains understudied. The purpose of this study is to explore the utilization of TI services by the transgender community in Uttarakhand.

Methods

This qualitative study focused on non-government organizations (NGOs) that implement TI projects in Haridwar and Roorkee, Uttarakhand, India. From September 2023 to January 2024, 5 focus group discussions (FGDs) involving 24 transgender women and 9 in-depth interviews (IDIs) involving NGO staff were conducted. Thematic analysis, guided by the Anderson and Newman healthcare utilization model, was employed.

Results

Several barriers to service utilization were identified, including ritual beliefs, low health literacy, stigma, social isolation, financial insecurity, privacy concerns, and inefficient service delivery. Challenges in the implementation of the STI program and neglect of transgender women in health facilities were also reported. However, enabling factors such as trusted providers, supportive laws, and initiatives by NGOs and government agencies were recognized. Nonetheless, there remains a significant need for information on gender-affirming services and addressing other prevalent health issues within the transgender female community.

Conclusion

The study underscores the interplay of individual, social, and service-related factors affecting healthcare access among transgender women. Inclusive and high-quality healthcare services are necessary to address the complex sociocultural aspects influencing transgender women’s healthcare access and utilization.
Unveiling microbial dynamics: a review of health and immune enhancement in school settings
Authors: Asumang Philip , Ntumi Richard , Dwomoh Francis
Published: December 2024

This review focuses on the role of microorganisms in promoting health and immune function within school environments. Microbes, including bacteria, viruses, fungi, and other microorganisms, constitute the human microbiome and play a crucial role in various bodily functions and immune system development. The complex interactions between microorganisms and the immune system in schools, where children spend a significant amount of time, are not fully understood. While schools have traditionally emphasized hygiene practices to prevent the spread of infectious diseases, recent research has highlighted the potential consequences of reduced microbial exposure during early life. The “hygiene hypothesis” suggests that limited exposure to microbes in infancy may increase the risk of allergies, asthma, and autoimmune diseases in adulthood. This paper explores the microbial diversity found in schools, the benefits of exposure to different microorganisms, and the implications of hygiene practices on immune system development. It also examines current research on microbial intervention strategies and their potential to influence overall health in schools. Understanding the role of microbes in school environments has implications for public health policies and educational practices, aiming to create healthier and more conducive learning environments for the younger generation. By comprehensively exploring this topic, this review contributes to a broader understanding of the significance of microbes in promoting health and immune function in school settings and its relevance to future health research.
Using crisis communications planning as part of a sustainable agritourism operation
Authors: Stearns Stacey F. , Singh-Knights Doolarie
Published: December 2024

Economic viability is a key factor to sustainability for agritourism operations. A variety of challenges, including issues and crises can hinder whether an operation is open, economically viable, and sustainable. A crisis communications plan can help address and minimize any negative business impacts from weather, climate, or inherent risk, therefore, helping maintain the operation's economic viability. Although many agritourism operators have experience responding to an issue or crisis, including the pandemic, many still lack communications plans, and while this is also a problem in other sectors, agritourism operations with plans have increased sustainability potential.
Moving beyond “believers vs. deniers” labels: a public response-centered framework for energy justice in the emerging renewable energy sector
Authors: Kunlere Idowu O. , Shah Kalim U.
Published: December 2024

The global shift toward renewable energy raises concerns that the legacy of energy injustice—historical imbalances in the distribution of benefits and burdens from fossil fuels—may persist within renewable systems intended to address climate change. Current research highlights that energy injustice could threaten the success of this transition, yet limited focus has been placed on understanding public responses to these emerging injustices. Existing frameworks often reduce public opinion to simplistic binaries (for example, “supporters vs. opponents”), which fail to capture the nuanced and diverse perspectives necessary for a comprehensive understanding of public sentiment on energy issues. To address this, we introduce the “PARO framework,” a novel tool that categorizes public responses to energy injustice within the renewable energy context. The PARO framework broadens the analytical lens on public opinion, offering insights that can guide more targeted and effective solutions for promoting equitable energy transitions.
Empowering sustainable development: revolutionizing agricultural green total factor productivity through rural digitalization
Authors: Zeng Fanjun , Zhou Yingying , Wei Bin
Published: December 2024

Introduction

The rapid advancement of digital technology has catalyzed the emergence of “digital villages,” presenting both new opportunities and challenges for the digital transformation of agriculture. This transformation has become a core driving force behind sustainable agricultural development. This paper utilizes panel data from 30 provincial-level administrative regions of mainland China (excluding Tibet) for the period 2011 to 2020 to explore how digital rural development (DRD) impacts agricultural green total factor productivity (AGTFP) and examines its underlying mechanisms.

Methods

Regarding variable measurement, the study employs the entropy weighting method to quantify the DRD and uses an extended Data Envelopment Analysis-Malmquist model to assess AGTFP. For the research approach, the study constructs a two-way fixed effects model to evaluate the impact of DRD on AGTFP and explores the underlying mechanisms. Specifically, the study conducts benchmark regression analysis, endogeneity tests, robustness tests, mechanism examinations, and heterogeneity tests.

Results

(1) DRD significantly enhances AGTFP, a conclusion that remains robust following the province-year clustering robust standard error estimates, winsorization tests, and excluding COVID-19-affected years. (2) Mechanistic analysis indicates that DRD primarily boosts AGTFP by promoting the development of digital inclusive finance (DIF) and facilitating the integration of primary, secondary, and tertiary industries (IPST). (3) Heterogeneity tests show that the positive impact of DRD on AGTFP is more pronounced in regions with higher levels of informatization and central regions of China.

Discussion

To harness DRD’s potential more effectively in fostering sustainable agricultural development, policymakers should concentrate on DIF and the IPST. Additionally, the government should pay close attention to disparities in informatization levels and resource allocation across regions and develop targeted strategies accordingly. Fundamentally, this study aims to elucidate China’s efficacy in rural digitalization and the green transformation of agriculture to an international audience. It seeks to provide insights and guidance on formulating more scientific, efficient, and sustainable strategies for agricultural development worldwide, thereby promoting green agricultural growth and supporting rural revitalization.
The impact of agricultural production agglomeration on agricultural economic resilience: based on spatial spillover and threshold effect test
Authors: Ranran Chao , Jingsuo Li
Published: December 2024

This study focuses on the role of agricultural production agglomeration in strengthening agricultural economic resilience, exploring the threshold effect of agricultural technological innovation level and the spatial spillover effect of agricultural production agglomeration on agricultural economic resilience. We conducted research across 31 provinces (including autonomous regions and municipalities) in China from 2007 to 2022. By constructing the evaluation index system of agricultural economic resilience, the entropy value method is used to measure the value of agricultural economic resilience, and then kernel density estimation and spatial econometrics model, threshold regression model are used to analyze the relationship between agricultural production agglomeration, agricultural technological innovation and agricultural economic resilience. (1) The analysis of the spatiotemporal evolution trend shows that the overall level of China’s agricultural economic resilience continued to rise, and presented a spatial development pattern of “high in the east and low in the west.” The overall level of agricultural production agglomeration in China shows a trend of first rising and then falling, among which the level of agricultural production agglomeration in the central region is significantly higher than that in the northwest and southeast regions. (2) The spatial Durbin model shows that agricultural production agglomeration can not only effectively improve the level of local agricultural economic resilience, but also have a positive impact on neighboring agriculture economic resilience produces positive spatial spillover effects. (3) Agricultural production agglomeration can improve the level of agricultural economic resilience by promoting agricultural social service. (4) The impact of agricultural production agglomeration on agricultural economic resilience shows great differences in different geographical regions. Among them, agricultural production agglomeration in the central region has a significant positive impact on the agricultural economic resilience of both local and adjacent areas. (5) The threshold effect model shows that the impact of agricultural production agglomeration on agricultural economic resilience has significant nonlinear characteristics, and its impact shows an increasing marginal effect as the level of agricultural technological innovation increases. To address this, policymakers should reinforce agricultural cluster construction, boost innovation capacity and treasure spillover effects between regions. These insights provide valuable direction for policymakers in crafting effective measures to enhance agricultural economic resilience.
A Centrality-Weighted Bidirectional Encoder Representation from Transformers Model for Enhanced Sequence Labeling in Key Phrase Extraction from Scientific Texts
Authors: Zengeya Tsitsi , Fonou Dombeu Jean Vincent , Gwetu Mandlenkosi
Published: December 2024

Deep learning approaches, utilizing Bidirectional Encoder Representation from Transformers (BERT) and advanced fine-tuning techniques, have achieved state-of-the-art accuracies in the domain of term extraction from texts. However, BERT presents some limitations in that it primarily captures the semantic context relative to the surrounding text without considering how relevant or central a token is to the overall document content. There has also been research on the application of sequence labeling on contextualized embeddings; however, the existing methods often rely solely on local context for extracting key phrases from texts. To address these limitations, this study proposes a centrality-weighted BERT model for key phrase extraction from text using sequence labelling (CenBERT-SEQ). The proposed CenBERT-SEQ model utilizes BERT to represent terms with various contextual embedding architectures, and introduces a centrality-weighting layer that integrates document-level context into BERT. This layer leverages document embeddings to influence the importance of each term based on its relevance to the entire document. Finally, a linear classifier layer is employed to model the dependencies between the outputs, thereby enhancing the accuracy of the CenBERT-SEQ model. The proposed CenBERT-SEQ model was evaluated against the standard BERT base-uncased model using three Computer Science article datasets, namely, SemEval-2010, WWW, and KDD. The experimental results show that, although the CenBERT-SEQ and BERT-base models achieved higher and close comparable accuracy, the proposed CenBERT-SEQ model achieved higher precision, recall, and F1-score than the BERT-base model. Furthermore, a comparison of the proposed CenBERT-SEQ model to that of related studies revealed that the proposed CenBERT-SEQ model achieved a higher accuracy, precision, recall, and F1-score of 95%, 97%, 91%, and 94%, respectively, than related studies, showing the superior capabilities of the CenBERT-SEQ model in keyphrase extraction from scientific documents.
Surface Behaviours of Humpback Whale Megaptera novaeangliae at Nosy Be (Madagascar)
Authors: Fabietti Ylenia , Spadaro Chiara , Tigani Agnese , Giglio Gianni , Barbuto Gianpiero , Romano Viviana , Fedele Giorgio , Leonetti Francesco Luigi , Venanzi Emanuele , Barba Carlotta , Sperone Emilio
Published: December 2024

The surface behaviours of humpback whales were studied in the presence of a whale-watching vessel at Nosy Be (Madagascar) during whale-watching activities, in order to characterise the ethogram of these animals. Data were collected from July to October 2018. Of the 75 total trips, humpback whales were observed 68 times and different types of aggregations were observed: Groups (33.82%), Mother–calf pairs (30.88%), Singles (27.94%), and Mother–calf and Escorts (7.35%). Individuals exhibited the following behaviours: Spouting, Breaching, Head Slap, Tail Throw, Tail Slap, Peck Slap, Spy-hopping, and Logging. Sighting data were evaluated by comparing the observed aggregations with reported behaviours, and vice versa. Among the most commonly observed behaviours, Spouting and Peck Slap were exhibited more in Groups, while Breaching was exhibited by all of the associations, with the exception of Singles. In Groups of more than two individuals, little or no social nor aggressive behaviours were observed, probably due to a lack of needing to attract the attention of other individuals. This suggests that, during the breeding season, Nosy Be could represent a wintering and weaning ground for calves.
DT-13 Mediates Ligand-Dependent Activation of PPARγ Response Elements In Vitro
Authors: Raina Shikha , Samuel Esther , Fuchs Hendrik
Published: December 2024

Activation of inflammatory pathways releases a storm of cytokines. Moreover, unregulated cytokines contribute to chronic inflammatory disorders. However, ligand-activated peroxisome proliferator-activated receptor gamma (PPARγ) is involved in suppressing inflammatory cytokines via transrepression of nuclear factor kappa B (NFκB). Therefore, in this study, the anti-inflammatory saponin DT-13 is explored as a ligand of PPARγ. DT-13 upregulated the expression of PPARγ in lipopolysaccharide (LPS)-stimulated RAW264.7 cells in comparison to treatment with LPS alone. Applying a HEK transfection model, we observed a DT-13 dose-dependent increase in ligand-dependent activation of PPARγ, which was compared with troglitazone and rosiglitazone. DT-13 was not able to compete with the synthetic fluoromone tracer for binding to PPARγ as observed in a fluorescence polarization binding assay, whereas molecular docking showed a possible binding interaction of DT-13 with the PPARγ nuclear receptor. We proved the expression of PPARγ protein in the presence of DT-13 using a robust cell-based HEK293FT transfection model. More in-depth analysis needs to be performed to evaluate the efficiency of the binding of DT-13 to PPARγ. A possible binding interaction of DT-13 to PPARγ was observed, similar to that of rosiglitazone. This study revealed a novel mechanism for anti-inflammatory effects by DT-13 through PPARγ-dependent transrepression of NFκB.