bluuebunny/crossref_metadata_2025 huggingface dataset:
Terrorism: Interpol Global Policing Goals and SDGs
Authors: Mordeson John N. , Mathew Sunil
Published: May 2024

In this paper, we determined how well an OECD country is achieving the Received Interpol goals with respect to the SDGs pertinent to them. We found that the Scandinavian countries were at the top in the achievement. In combatting terrorism in general, we found that the Scandinavian countries were at the top. We also show that the fuzzy similarity measure of the ranking of countries used by the SDGs relevant to terrorism compared with SDG 16 alone was high. Let [Formula: see text] and [Formula: see text] be rankings of a set [Formula: see text] and [Formula: see text] and [Formula: see text] the associated fuzzy subsets, respectively. Consider the similarity measures [Formula: see text] and [Formula: see text] [J. N. Mordeson and S. Mathew, Journal of Mahani Mathematical Research 13(1) (2024) 279–291]. Suppose that [Formula: see text] and [Formula: see text] are known, but [Formula: see text] is unknown. It is shown in the reference by J. N. Mordeson and S. Mathew [Mathematics of Uncertainty for Coping with World Challenges, Climate Change, World Hunger, Modern Slavery, Coronavirus, Human Trafficking (Springer, 2021)] that if  [Formula: see text] is near [Formula: see text] then [Formula: see text] is near [Formula: see text] In this paper, a similar result for [Formula: see text] is shown.
Nature Loss in Southeast Asia: Aichi Targets and EPI
Authors: Mordeson John N. , Mathew Sunil , Gayathri G.
Published: May 2024

The Aichi biodiversity targets were established by the UN convention of biological diversity and consist of 20 specific targets to address and mitigate biodiversity loss across the globe. We consider countries in East and South Asia. Countries in East and South Asia are very different in size and level of economic development. As such Sustainable Development Goal challenges vary greatly across countries. The 2022 Environmental Performance Index (EPI) provides a quantitative basis for comparing, analyzing, and understanding environmental performance for 180 countries. The EPI score and rank these countries on their environmental performance using the most recent year of data available and calculate how these scores have changed over the previous decade. We determine the similarity between the country achievement rankings of the Aichi Targets and the EPI rankings.
Complex Dynamics and Bifurcations Analysis of Discrete-Time Modified Leslie–Gower System
Authors: Singh Anuraj , Parwaliya Ankit , Kumar Ajay , Elsonbaty Amr , Elsadany A. A.
Published: May 2024

This work introduces a discrete modified Leslie–Gower prey–predator system with Holling type-II functional response. The persistence of the discrete model under certain conditions is discussed. The conditions assuring the existence of fixed points are derived and nonlinear dynamics of system are explored at these fixed points. It has been shown that the system exhibits transcritical bifurcation and flip bifurcation at semi-trivial fixed point under certain bifurcation values. In addition, the center manifold and bifurcation theories are employed to attain the conditions for existence of flip and Neimark–Sacker bifurcations at coexistence fixed point. The system is found to exhibit periodic solutions along with bifurcations leading to wide range of chaotic dynamics. The numerical simulations are performed to confirm the analytical analysis.
Multi-Hop Multi-View Memory Transformer for Session-Based Recommendation
Authors: Zhuo Xingrui , Qian Shengsheng , Hu Jun , Dai Fuxin , Lin Kangyi , Wu Gongqing
Published: May 2024

A Session-Based Recommendation (SBR) seeks to predict users’ future item preferences by analyzing their interactions with previously clicked items. In recent approaches, Graph Neural Networks (GNNs) have been commonly applied to capture item relations within a session to infer user intentions. However, these GNN-based methods typically struggle with feature ambiguity between the sequential session information and the item conversion within an item graph, which may impede the model’s ability to accurately infer user intentions. In this article, we propose a novel Multi-hop Multi-view Memory Transformer (M
3
T) to effectively integrate the sequence-view information and relation conversion (graph-view information) of items in a session. First, we propose a Multi-view Memory Transformer (M
2
T) module to concurrently obtain multi-view information of items. Then, a set of trainable memory matrices are employed to store sharable item features, which mitigates cross-view item feature ambiguity. To comprehensively capture latent user intentions, an M
3
T framework is designed to integrate user intentions across different hops of an item graph. Specifically, a k-order power method is proposed to manage the item graph to alleviate the over-smoothing problem when obtaining high-order relations of items. Extensive experiments conducted on three real-world datasets demonstrate the superiority of our method.
Review—Nanotechnology in Aquaculture: Applications and Challenges
Authors: Nguyen Minh Quang , Nguyen Do Mai , Toan Tran Thanh Tam , Dao Anh Quang
Published: May 2024

Aquaculture, driven by increasing demands for animal proteins and fats, faces multifaceted challenges stemming from environmental factors such as climate change and pollution, alongside issues like disease susceptibility and limited therapeutic tools. However, the emergence of nanotechnology (NNT) offers a promising solution across various aquaculture domains. Nano-enhanced feed has been shown to improve fish growth rates, while nanomaterials are reducing the treatment economy by effectively eliminating contaminants. Genetic manipulation methods combined with nanobiotechnology have revolutionized fish ancestry studies, with advancements such as nanosensors and DNA-based vaccines significantly impacting fish life and immune systems. Moreover, nanotechnology plays a crucial role in enhancing fish processing, enabling sterile packaging and precise flavoring. Utilizing fishery waste through bio-nano-engineering and green nanoparticles offers new post-harvesting practices. Despite ongoing exploration, NNT presents versatile applications, prospects, and challenges in aquaculture, as detailed in this review. This paper provides an in-depth analysis of current trends, challenges, and prospects of NNT applications in aquaculture.
Intergranular Attack of Low Carbon Steel in Molten Aluminum Chloride
Authors: Lu Mingquan , Leman John , Lewis Daniel , Duquette David
Published: May 2024

AISI 1018 carbon steel exhibits intergranular attack in molten aluminum chloride. To explore grain boundary corrosion initiation and propagation, tests have been conducted on several iron-based alloys, heat treated to recrystallization temperature, and using molten aluminum chloride and its mixture with other molten chlorides environments. Pure iron, A106, and AISI 1018 carbon steel have been exposed to both pure aluminum chloride and ferric chloroaluminate melt in both their recrystallized and as-received, cold-worked conditions. Intergranular corrosion is observed in both 1018 and A106 carbon steels in all the salts whereas pure iron only shows pitting. Materials processing has varying effects on the corrosion depths of 1018 and A106 carbon steels. The grain boundary microchemistry of 1018 carbon steel is examined with in situ fracture Auger spectroscopy where molybdenum and carbon segregation are found, and a mechanism is proposed to explain the present corrosion phenomenon.
AGPAT3 deficiency impairs adipocyte differentiation and leads to a lean phenotype in mice
Authors: Zhou Hongyi , Fick Kendra , Patel Vijay , Hilton Lisa Renee , Kim Ha Won , Bagi Zsolt , Weintraub Neal L. , Chen Weiqin
Published: May 2024

AGPAT3 deficiency results in male-specific growth retardation. It reduces adipose tissue mass but does not significantly impact glucose homeostasis or energy balance, except for influencing water intake in mice. Like AGPAT2, AGPAT3 is upregulated during adipogenesis, potentially by peroxisome proliferator-activated receptor gamma (PPARγ). Loss of AGPAT3 impairs adipocyte differentiation, which could be rescued by pioglitazone. Overall, AGPAT3 plays a significant role in regulating adipose tissue mass, partially involving its influence on adipocyte differentiation.
Chemical Composition and In Vitro and In Silico Biological Activities of Myrciaria tenella (DC.) O.Berg (Myrtaceae) Essential Oil from Brazil
Authors: Ferreira Oberdan Oliveira , Costa Vanessa Guimarães , Cruz Jorddy Nevez , Kataoka Maria Sueli da Silva , Pinheiro João de Jesus Viana , Nascimento Lidiane Diniz do , Varela Everton Luiz Pompeu , Cascaes Márcia Moraes , Percário Sandro , Mali Suraj N. , Menezes Tatiany Oliveira de Alencar , de Oliveira Mozaniel Santana , Andrade Eloisa Helena de Aguiar
Published: May 2024

Myrciariatenella O.Berg, a native plant species of Brazil, exhibits pharmacological applications, including antitumor activity. In this study, we isolated the essential oil (EO) of M. tenella and identified its phytochemical profile. In addition, we determined the in vitro and in silico cytotoxic activities of EO in nontumor and tumor cell lines (gingival fibroblasts and oral squamous cell carcinoma, respectively) and its free radical scavenging activity (i.e., antioxidant activity) using ABTS and DPPH assays. The EO of M. tenella primarily constitutes hydrocarbons and oxygenated sesquiterpenes, with (E)-caryophyllene (33.95%), δ-cadinene (7.4%), caryophyllene oxide (4.74%), and viridiflorene (4.49%) as its four major components. EO effectively suppressed the cell viability of CAL-27 tumor cells to below 70% at concentrations of 125 and 250 μg/mL and exhibited a free radical inhibition potential of 75.63 ± 0.41% and 28.46 ± 0.36%, respectively, in the DPPH and ABTS assays. This chemical and biological potential may be attributed to the major compounds present in EO, as well as the molecular coupling simulations conducted, which revealed the anticancer mechanism of EO in the sesquiterpenes (E)-caryophyllene, δ-cadinene, caryophyllene oxide, and viridiflorene.
Effects of SiO2 Nanoparticles on Polyvinyl Alcohol/Carboxymethyl Cellulose Polymer Blend Films’ Structural, Wettability, Surface Roughness, and Optical Characteristics
Authors: Soliman T. S.
Published: May 2024

The blend matrix composed of polyvinyl alcohol and carboxymethylcellulose (PVA/CMC) was prepared via the casting method. SiO2 nanoparticles were added as reinforcement in different amounts (SiO2 = 1, 2, 3, and 4 wt.%). The study utilized FTIR to examine the alterations in composition and the interplay between the blend matrix and the inclusion of SiO2. Also, for the first time, the surface roughness and surface wettability of the PVA/CMC blend matrix were investigated with the addition of SiO2 using measurements of contact angle and surface roughness parameters. The surface roughness and wettability of the blend matrix increased as the SiO2 content increased. In addition, the blend matrix optical features were determined by the UV–visible spectrophotometer. Based on the analysis using Tauc’s relation, it was found that the energy bandgap decreases from 5.52 to 5.17 eV (direct transition) and from 4.79 to 4.32 eV (indirect transition) for the PVA/CMC and PVA/CMC/4%SiO2 blend films, respectively. The refractive index increases from 2.009 to about 2.144 for the PVA/CMC and PVA/CMC/4%SiO2 blend films, respectively. Furthermore, optical conductivity and dielectric constants were improved for the PVA/CMC blend film after the addition of SiO2 nanoparticles.
Seroprevalence of Fasciola sp. and Toxoplasma gondii Infections in Rural and Urban Inhabitants of Jolfa County, Northwest Iran
Authors: Zeinali Shiva , Jafari Rasool , Khademvatan Shahram , Sakhaei Ghorban , Masudi Sima , Khashaveh Shahla , Asadi Negar , Yousefi Elham
Published: May 2024

Fascioliasis and toxoplasmosis are the two important zoonotic diseases that are endemic in Iran and share some common transmission routes. The present study is aimed at determining the seroprevalence of human fascioliasis and toxoplasmosis in rural and urban areas of Jolfa County, Northwest Iran. In a cross-sectional study, 600 human sera were collected randomly from humans living in Jolfa County including three cities and 13 villages from 2017 to 2018. Anti-Toxoplasma IgG and anti-Fasciola sp. IgG tests have been performed using the enzyme-linked immunosorbent assay. Four (0.7%) out of 600 human sera showed positive levels of anti-Fasciola IgG. Three out of four seropositive humans were from an urban area, and one (25%) was from rural inhabitants. Considering T. gondii infection, 45% of studied human sera were seropositive for anti-T. gondii IgG. In conclusion, this is the first study reporting Fasciola seropositivity in the area. Based on the findings, human fascioliasis is present in the studied area, Northwest Iran, granted in low prevalence. Considering T. gondii seropositivity, the prevalence is high, yet close to the reports from other regions in the province.
Development and Evaluation of Monoclonal Antibodies against CBPP Antigen with the End Goal of Developing an ELISA Kit
Authors: Ramathudi-Dunbar Lorato , Awosanya Emmanuel , Charles Sanne Bodjo , Chitsungo Ethel , Moustapha Boukary Cisse Rahamatou , Nwankpa Nick , Gelaw Hassen , Tessema Yebechaye , Melesse A. Gelagay , Rayson Sanga Richard , Bright Adorbley , Baziki Jean de Dieu
Published: May 2024

Contagious bovine pleuropneumonia (CBPP) is an infectious and contagious bacterial respiratory disease that affects cattle with significant economic losses to the African animal industry. The use of ELISA kits based on monoclonal antibodies (mAbs) will aid in quick and precise diagnosis of CBPP, contributing to disease control and prevention in cattle. Thus, this research aims to develop and evaluate monoclonal antibodies against CBPP (T1/44) antigen for use in ELISA kits for CBPP diagnosis. Hybridoma technology was used to develop monoclonal antibodies that recognize and bind to the CBPP (T1/44) antigen. The antibody‐secreting hybridomas were produced after immunizing mice with purified CBPP antigens. The hybridomas were screened for high sensitivity, specificity, and liking to the antigen. The selected mAbs were assessed for sensitivity and specificity against CBPP antigen using different immunoassays, dot‐blot, ELISA, and mouse mAb isotyping. The monoclonal antibodies were profoundly specific, with a higher hindrance to CBPP antigen (<0.50 OD) while lacking cross‐reactivity to other antigens. The monoclonal antibodies could distinguish CBPP antigen at low concentrations, showing their high sensitivity (>80% PI). The isotyped mAbs of intrigued appeared to have a place in the IgG class. These identified monoclonal antibodies can be utilized to develop an ELISA kit for CBPP diagnosis, which would give a fast, precise, and cost‐effective strategy for screening and checking CBPP in cattle herds.
Impact of Randomized Soil Properties and Rock Motion Intensities on Ground Motion
Authors: Chala Ayele , Ray Richard
Published: May 2024

Seismic site response is inevitably influenced by natural variability of soil properties and anticipated earthquake intensity. This study presents the influence of variability in shear wave velocity (Vs) and amplitude of input rock motion on seismic site response analysis. Monte Carlo simulations were employed to randomize the Vs profile for different scenarios. A series of 1‐D equivalent linear (EQL) seismic site response analyses were conducted by combining the randomized Vs profile with different levels of rock motion intensities. The results of the analyses are presented in terms of surface spectral acceleration, amplification factors (AFs), and peak ground acceleration (PGA). The mean and standard deviation of these parameters are thoroughly discussed for a wide range of randomized Vs profile, number of Vs randomizations, and intensities of input rock motions. The results demonstrate that both the median PGA and its standard deviations across different number of Vs profile realization exhibit a slight variation. As few as twenty Vs profile realizations are sufficient to compute reliable response parameters. Both rock motion intensity and standard deviation of Vs variability cause significant variation in computed surface parameters. However, the variability in the number of records used to conduct site response has no significant impact on ground response if the records closely match the target spectrum. Incorporating the multiple sources of variabilities can reduce uncertainty when conducting ground response simulations.
A Virus-Inspired Inhalable Liponanogel Induces Potent Antitumor Immunity and Regression in Metastatic Lung Tumors
Authors: Li Junyao , Luo Lanqing , He Jia , Yu Jinchao , Li Xinyan , Shen Xueying , Zhang Junxia , Li Sai , Karp Jeffrey M. , Kuai Rui
Published: May 2024

Pulmonary delivery of immunostimulatory agents such as poly(I:C) to activate double-stranded RNA sensors MDA5 and RIG-I within lung-resident antigen-presenting cells is a potential strategy to enhance antitumor immunity by promoting type I interferon secretion. Nevertheless, following pulmonary delivery, poly(I:C) suffers from rapid degradation and poor endosomal escape, thus limiting its potency. Inspired by the structure of a virus that utilizes internal viral proteins to tune the loading and cytosolic delivery of viral nucleic acids, we developed a liponanogel (LNG)–based platform to overcome the delivery challenges of poly(I:C). The LNG comprised an anionic polymer hyaluronic acid–based nanogel core coated by a lipid shell, which served as a protective layer to stabilize the nanogel core in the lungs. The nanogel core was protonated within acidic endosomes to enhance the endosomal membrane permeability and cytosolic delivery of poly(I:C). After pulmonary delivery, LNG-poly(I:C) induced 13.7-fold more IFNβ than poly(I:C) alone and two-fold more than poly(I:C) loaded in the state-of-art lipid nanoparticles [LNP-poly(I:C)]. Additionally, LNG-poly(I:C) induced more potent CD8+ T-cell immunity and stronger therapeutic effects than LNP-poly(I:C). The combination of LNG-poly(I:C) and PD-L1 targeting led to regression of established lung metastases. Due to the ease of manufacturing and the high biocompatibility of LNG, pulmonary delivery of LNG may be broadly applicable to the treatment of different lung tumors and may spur the development of innovative strategies for cancer immunotherapy.

Significance: Pulmonary delivery of poly(I:C) with a virus-inspired inhalable liponanogel strongly activates cytosolic MDA5 and RIG-I and stimulates antitumor immunity, representing a promising strategy for safe and effective treatment of metastatic lung tumors.
Combining EHMT and PARP Inhibition: A Strategy to Diminish Therapy-Resistant Ovarian Cancer Tumor Growth while Stimulating Immune Activation
Authors: Nguyen Lily L. , Watson Zachary L. , Ortega Raquel , Woodruff Elizabeth R. , Jordan Kimberly R. , Iwanaga Ritsuko , Yamamoto Tomomi M. , Bailey Courtney A. , To Francis , Jeong Abigail D. , Guntupalli Saketh R. , Behbakht Kian , Gibaja Veronica , Arnoult Nausica , Cocozaki Alexis , Chuong Edward B. , Bitler Benjamin G.
Published: May 2024

Despite the success of poly-ADP-ribose polymerase inhibitors (PARPi) in the clinic, high rates of resistance to PARPi presents a challenge in the treatment of ovarian cancer, thus it is imperative to find therapeutic strategies to combat PARPi resistance. Here, we demonstrate that inhibition of epigenetic modifiers euchromatic histone lysine methyltransferases 1/2 (EHMT1/2) reduces the growth of multiple PARPi-resistant ovarian cancer cell lines and tumor growth in a PARPi-resistant mouse model of ovarian cancer. We found that combinatory EHMT and PARP inhibition increases immunostimulatory double-stranded RNA formation and elicits several immune signaling pathways in vitro. Using epigenomic profiling and transcriptomics, we found that EHMT2 is bound to transposable elements, and that EHMT inhibition leads to genome-wide epigenetic and transcriptional derepression of transposable elements. We validated EHMT-mediated activation of immune signaling and upregulation of transposable element transcripts in patient-derived, therapy-naïve, primary ovarian tumors, suggesting potential efficacy in PARPi-sensitive disease as well. Importantly, using multispectral immunohistochemistry, we discovered that combinatory therapy increased CD8 T-cell activity in the tumor microenvironment of the same patient-derived tissues. In a PARPi-resistant syngeneic murine model, EHMT and PARP inhibition combination inhibited tumor progression and increased Granzyme B+ cells in the tumor. Together, our results provide evidence that combinatory EHMT and PARP inhibition stimulates a cell autologous immune response in vitro, is an effective therapy to reduce PARPi-resistant ovarian tumor growth in vivo, and promotes antitumor immunity activity in the tumor microenvironment of patient-derived ex vivo tissues of ovarian cancer.
Long Noncoding RNA MSL3P1 Regulates CUL3 mRNA Cytoplasmic Transport and Stability and Promotes Lung Adenocarcinoma Metastasis
Authors: Shao Ming-Ming , Li Xin , Wei Rui-Qi , Chen Qing-Yu , Zhang Xin , Qiao Xin , Li Hui
Published: May 2024

Lung adenocarcinoma (LUAD) is the most prevalent histological type of lung cancer. Previous studies have reported that specific long noncoding RNAs (lncRNA) are involved in cancer development and progression. The phenotype and mechanism of ENST00000440028, named MSL3P1, an lncRNA referred to as a cancer-testis gene with potential roles in tumorigenesis and progression, have not been reported. MSL3P1 is overexpressed in LUAD tumor tissues, which is significantly associated with clinical characteristics, metastasis, and poor clinical prognosis. MSL3P1 promotes the metastasis of LUAD in vitro and in vivo. The enhancer reprogramming in LUAD tumor tissue is the major driver of the aberrant expression of MSL3P1. Mechanistically, owing to the competitive binding to CUL3 mRNA with ZFC3H1 protein (a protein involved in targeting polyadenylated RNA to exosomes and promoting the degradation of target mRNA), MSL3P1 can prevent the ZFC3H1-mediated RNA degradation of CUL3 mRNA and transport it to the cytoplasm. This activates the downstream epithelial-to-mesenchymal transition signaling pathway and promotes tumor invasion and metastasis.

Implications: This study indicates that lncRNA MSL3P1 regulates CUL3 mRNA stability and promotes metastasis and holds potential as a prognostic biomarker and therapeutic target in LUAD.
Dermoscopic Nail Changes in Psoriasis, Lichen Planus, and Lichen Striatus
Authors: Sar-Pomian Marta , Starace Michela V.R. , Lencastre Andrè , Piraccini Bianca Maria , Richert Bertrand , Rudnicka Lidia , Trakatelli Myrto Georgia , Iorizzo Matilde
Published: May 2024

Background: Onychoscopy is a noninvasive method helpful in diagnosing nail disorders. The aim of the study was to review literature on the usability of onychoscopy in nail psoriasis, nail lichen planus, and nail lichen striatus. Summary: Onychoscopic features of nail psoriasis are pitting, onycholysis with erythematous border, salmon patches, splinter hemorrhages, dotted vessels in lateral and proximal folds, and hyponychium. Onychoscopic features of nail lichen planus are onychorrhexis, onycholysis, longitudinal melanonychia, and red lunula. The literature on the usability of onychoscopy in nail lichen striatus is scarce. Keynotes: Onychoscopy facilitates evaluation of nail abnormalities compared to the clinical examination. Lunular alterations, salmon patches, erythematous border of onycholysis as well as splinter hemorrhages in nail psoriasis are better visualized with onychoscopy compared to the naked eye. Onychoscopy enhances detection of melanonychia, dyschromia, and lunular changes in nail lichen planus. Onychoscopic features are different in fingernails and toenails.
Clinically Applicable Pan-Origin Cancer Detection for Lymph Nodes via Artificial Intelligence-Based Pathology
Authors: Pan Yi , Dai Hongtian , Wang Shuhao , Wang Lang , Li Qiting , Wang Wenmiao , Li Jiangtao , Qi Dan , Yang Zhaoyang , Jia Jia , Wang Yaxi , Fang Qing , Li Lin , Zhou Weixun , Song Zhigang , Zou Shuangmei
Published: May 2024

Introduction: Lymph node metastasis is one of the most common ways of tumour metastasis. The presence or absence of lymph node involvement influences the cancer’s stage, therapy, and prognosis. The integration of artificial intelligence systems in the histopathological diagnosis of lymph nodes after surgery is urgent. Methods: Here, we propose a pan-origin lymph node cancer metastasis detection system. The system is trained by over 700 whole-slide images (WSIs) and is composed of two deep learning models to locate the lymph nodes and detect cancers. Results: It achieved an area under the receiver operating characteristic curve (AUC) of 0.958, with a 95.2% sensitivity and 72.2% specificity, on 1,402 WSIs from 49 organs at the National Cancer Center, China. Moreover, we demonstrated that the system could perform robustly with 1,051 WSIs from 52 organs from another medical centre, with an AUC of 0.925. Conclusion: Our research represents a step forward in a pan-origin lymph node metastasis detection system, providing accurate pathological guidance by reducing the probability of missed diagnosis in routine clinical practice.
Peripheral 5-HT Mediates Gonadotropin-Inhibitory Hormone-Induced Feeding Behavior and Energy Metabolism Disorder in Chickens via the 5-HT2C Receptor
Authors: Song Xingxing , Xu Wenhao , Li Zixin , Zhang Xin , Liu Chengcheng , Han Kaiou , Chen Lei , Shi Yan , Xu Changlin , Han Dongyang , Luo Rongrong , Cao Yajie , Li Qingwen , Yang Huihua , Lu Qiucheng , Qin Jin , Wang Xiaoye , Hu Chuanhuo , Li Xun
Published: May 2024

Introduction: Since the discovery of gonadotropin-inhibitory hormone (GnIH), it has been found to play a critical role in reproduction in vertebrates. Recently, a regulatory role of GnIH in appetite and energy metabolism has emerged, although its precise physiological mechanisms remain unknown. Methods: Thus, the present study evaluated the effects of a single or long-term intraperitoneal GnIH treatment on the food intake, weight, and glucolipid metabolism of chickens, as well as investigating the possible neuroendocrinology factors and mechanisms involved in GnIH-induced obesity and glucolipid metabolism disorder. Results: Our results show that the intraperitoneal administration of GnIH to chickens resulted in a marked body mass increase, hyperlipidemia, hyperglycemia, and glucose intolerance. Subsequently, the results of metabolomics studies and the pharmacological inhibition of the 5-HT2C receptor revealed that blocking the 5-HT2C receptor reinforced the effects of GnIH on food intake, body weight, and blood glucose and lipid levels, resulting in even worse cases of GnIH-induced hyperglycemia, hyperlipidemia, and hepatic lipid deposition. This suggests that, via the 5-HT2C receptor, peripheral 5-HT may act as a negative feedback regulator to interplay with GnIH and jointly control energy balance homeostasis in chickens. Discussion: Our present study provides evidence of cross-talk between GnIH and 5-HT in food intake and energy metabolism at the in vivo pharmacological level, and it proposes a molecular basis for these interactions, suggesting that functional interactions between GnIH and 5-HT may open new avenues for understanding the mechanism of the neuroendocrine network involved in appetite and energy metabolism, as well as providing a new therapeutic strategy to prevent obesity, diabetes, and metabolic disorders.
A Novel 3D High Resolution Imaging Method Using Correlative X-Ray and Electron Microscopy to Study Neodymium-Doped Yttrium Aluminum Garnet Laser-Induced Defects in Intraocular Lenses
Authors: Borkenstein Andreas F. , Mikitisin Adrian , Schwedt Alexander , Borkenstein Eva-Maria , Mayer Joachim
Published: May 2024

Introduction: Cataract extraction is the most frequently performed ophthalmological procedure worldwide. Posterior capsule opacification remains the most common consequence after cataract surgery and can lead to deterioration of the visual performance with cloudy, blurred vision and halo, glare effects. Neodymium-doped yttrium aluminum garnet (Nd:YAG) laser capsulotomy is the gold standard treatment and a very effective, safe and fast procedure in removing the cloudy posterior capsule. Damaging the intraocular lens (IOL) during the treatment may occur due to wrong focus of the laser beam. These YAG-pits may lead to a permanent impairment of the visual quality. Methods: In an experimental study, we intentionally induced YAG pits in hydrophilic and hydrophobic acrylic IOLs using a photodisruption laser with 2.6 mJ. This experimental study established a novel 3D imaging method using correlative X-ray and scanning electron microscopy (SEM) to characterize these damages. By integrating the information obtained from both X-ray microscopy and SEM, a comprehensive picture of the materials structure and performance could be established. Results: It could be revealed that although the exact same energies were used to all samples, the observed defects in the tested lenses showed severe differences in shape and depth. While YAG pits in hydrophilic samples range from 100 to 180 µm depth with a round shape tip, very sharp tipped defects up to 250 µm in depth were found in hydrophobic samples. In all samples, particles/fragments of the IOL material were found on the surface that were blasted out as a result of the laser shelling. Conclusion: Defects in hydrophilic and hydrophobic acrylic materials differ. Material particles can detach from the IOL and were found on the surface of the samples. The results of the laboratory study illustrate the importance of a precise and careful approach to Nd:YAG capsulotomy in order to avoid permanent damage to the IOL. The use of an appropriate contact glass and posterior offset setting to increase safety should be carried out routinely.
Vascular Complications in Children and Young People with Type 1 Diabetes: A Worldwide Assessment of Diabetologists’ Adherence to International Recommendations
Authors: Piona Claudia , Chobot Agata , Dos Santos Tiago Jeronimo , Giani Elisa , Marcovecchio M. Loredana , Maffeis Claudio , de Beaufort Carine
Published: May 2024

Introduction: This global survey evaluated the practices and adherence to international Clinical Practice Consensus Guidelines (CPCG) of physicians involved in pediatric diabetes care regarding screening, prevention and treatment of vascular complications of type 1 diabetes (T1D). Method: A web-based survey gathering data about respondents’ background, practices related to screening, prevention, and treatment of diabetic nephropathy, retinopathy, neuropathy, and macrovascular diseases and a self-assessment of physicians’ knowledge based on the ISPAD CPCG 2018 were shared by ISPAD. Results: We received 175 responses from 62 countries (60% female, median age 42.3 years, 72.0% ISPAD members). Two-thirds of respondents initiated nephropathy and retinopathy screening per CPCG recommendations. Only half of them adhered to recommendations for neuropathy and macrovascular disease risk factors (RFs). Over 85% of respondents used the recommended screening method for nephropathy, retinopathy and macrovascular disease RFs, and only 59% for neuropathy. Lack of access to neuropathy and macrovascular diseases RF screening methods was reported by 22.2% and 11.8% of respondents, respectively. Adherence to recommended screening frequency varied: 92% for nephropathy, around two-thirds for neuropathy and macrovascular disease RFs, and only 17.7% for retinopathy. Most participants aligned their practices for treating T1D complications with CPCG recommendations, except for nephropathy. Significant differences in adherence to CPCG and individuals’ financial contributions reflected countries' income levels. Around 50% of the respondents were very familiar with the ISPAD CPCG content. Conclusion: Our study highlights global variation in adherence to CPCG for T1D vascular complications, which is influenced by country income and healthcare disparities. It also revealed knowledge gaps among physicians on this critical topic.
Neurodevelopmental Follow-Up of Children Born to Mothers with Graves’ Disease and Neonatal Hyperthyroidism
Authors: Grob Francisca , Brown Amy , Zacharin Margaret
Published: May 2024

Introduction: Neonatal hyperthyroidism, often caused by maternal Graves’ disease (GD), carries potential neurodevelopmental risks for children. Excessive thyroid hormones during fetal development are linked to neurological issues like ADHD and epilepsy. However, the impact of transient neonatal hyperthyroidism is not well understood. Methods: In a retrospective study at the Royal Children’s Hospital in Melbourne, 21 neonates with hyperthyroidism from mothers with GD were examined. Of these, the parents of 10 children consented to participate; thus, questionnaires assessing executive functions, behavior, and social communication were completed. The outcomes were compared to those of control subjects recruited from the community using standardized tools (BRIEF, SDQ, SCQ). The results were analyzed against socio-demographic factors, maternal, and neonatal health. Results: No significant demographic or clinical differences were found between study participants (n = 10) and non-participants (n = 11). Participants, compared to controls, showed similar family demographics but a higher proportion of control parents had university-level education (p = 0.003). Patients displayed more social (SCQ scores: 12.1 ± 2.5 vs. 6 ± 1.07, p = 0.008) and behavioral difficulties (SDQ scores: 10.2 ± 2.17 vs. 6.14 ± 1.03, p = 0.03), with increased executive function challenges (BRIEF scores indicating problem-solving and self-regulation difficulties). Significant effects of family living situation and partner education level on neurodevelopmental measures were noted, underscoring the influence of socio-demographic factors. Conclusions: These findings suggest neonatal hyperthyroidism might lead to subtle neurodevelopmental variations, with socio-economic elements and family dynamics possibly intensifying these effects. While most children did not show severe impairments, early detection and intervention are recommended. The research emphasizes the necessity for inclusive care approaches that consider socio-economic factors for children affected by neonatal hyperthyroidism.
First record of Pyrgotidae (Diptera, Tephritoidea) from Uruguay with two new synonyms in the genus Leptopyrgota Hendel
Authors: Mello Ramon Luciano
Published: May 2024

Pyrgotidae are a small family of Tephritoidea of worldwide distribution, whose larvae are known to be endoparasites in the abdomen of adult Scarabaeidae beetles. This study presents the first record of the family from Uruguay, represented by the Leptopyrgota albitarsis Aczél, 1956 from Treinta y Tres, Quebrada de los Cuervos. Based on the study of holotypes, the following synonymy is proposed: Leptopyrgota albitarsis Aczél, 1956 = Leptopyrgota definienda Bernardi, 1991, new synonym and L. liae Bernardi, 1991 new synonym. Photos of the holotypes of the three studied species are presented, for the first time.
Geographic variation in the acoustic signals of lesser treefrogs Dendropsophus minutus (Anura, Hylidae)
Authors: Chaves Maycon Veiga , Sena Vitor Matheus Alcântara de , Diniz Pedro
Published: May 2024

Biological sound archives are a major source to investigate geographic variation in animal acoustic signals and their evolutionary drivers. The acoustic signals of anuran species with wide distribution ranges often vary geographically as a result of isolation by distance and climate amongst other factors. We examined whether the acoustic structure of call notes would vary geographically in lesser treefrogs Dendropsophus minutus using recordings from bioacoustics repositories. We also tested whether climate (mean annual temperature and annual precipitation) drive geographic variation in those signals. The acoustic distance was unrelated to geographic distance, suggesting that isolation by distance solely cannot explain geographic variation in call structure. Overall, lesser treefrogs uttered call notes with lower frequencies and bandwidths in the west of their range. In addition, frogs produced shorter call notes in hotter, wetter sites and narrow-bandwidth notes in hotter environments. We suggest that frogs produce more calls (not measured here) of shorter durations to maximize transmission and minimize the metabolic costs of calling at high air temperatures. We also suggest that hotter environments favor the propagation of lower-bandwidth calls. This study reinforces the feasibility and power of citizen science and acoustic data repositories for understanding the evolution of anuran acoustic signals.
Normative Values of Echocardiographic Chamber Size and Function in Older Healthy Adults: The Multi-Ethnic Study of Atherosclerosis
Authors: Mukherjee Monica , Strom Jordan B. , Afilalo Jonathan , Hu Mo , Beussink-Nelson Lauren , Kim Jiwon , Addetia Karima , Bertoni Alain G. , Gottdiener John S. , Michos Erin D. , Gardin Julius M. , Shah Sanjiv J. , Freed Benjamin H.
Published: May 2024

BACKGROUND:

Echocardiographic (2-dimensional echocardiography) thresholds indicating disease or impaired functional status compared with normal physiological aging in individuals aged ≥65 years are not clearly defined. In the present study, we sought to establish standard values for 2-dimensional echocardiography parameters related to chamber size and function in older adults without cardiopulmonary or cardiometabolic conditions.

METHODS:

In this cross-sectional study of 3032 individuals who underwent 2-dimensional echocardiography at exam 6 in the MESA (Multi-Ethnic Study of Atherosclerosis), 608 participants fulfilled our inclusion criteria of healthy aging, with normative values defined as the mean ± 1.96 standard deviation and compared across sex and race and ethnicity. Functional status measures included NT-proBNP (N-terminal pro-B-type natriuretic peptide), 6-minute walk distance, and Kansas City Cardiomyopathy Questionnaire. Prognostic performance using MESA cutoffs was compared with established guideline cutoffs using time-to-event analysis.

RESULTS:

The normative aging cohort (69.5±7.0 years, 46.2% male, 47.5% White) had lower NT-proBNP, higher 6-minute walk distance, and higher (better) Kansas City Cardiomyopathy Questionnaire summary values. Women had significantly smaller chamber sizes and better biventricular systolic function. White participants had the largest chamber dimensions, whereas Chinese participants had the smallest, even after adjustment for body size. Current guidelines identified 81.6% of healthy older adults in MESA as having cardiac abnormalities.

CONCLUSIONS:

Among a large, diverse group of healthy older adults, we found significant differences in cardiac structure and function by sex and race/ethnicity, which may signal sex-specific cardiac remodeling with advancing age. It is crucial for existing guidelines to consider the observed and clinically significant differences in cardiac structure and function associated with healthy aging. Our study highlights that existing guidelines, which grade abnormalities in echocardiographic cardiac chamber size and function based on younger individuals, may not adequately address the anticipated changes associated with normal aging.
Overexpressed Trophoblast Glycoprotein Contributes to Preeclampsia Development by Inducing Abnormal Trophoblast Migration and Invasion Toward the Uterine Spiral Artery
Authors: Sun Lu , Shi Meiting , Wang Jian , Han Xiaoxue , Wei Jiachun , Huang Zhengrui , Yang Xiaofeng , Ding Yuzhen , Zhang Ping , He Andong , Liu Mengyuan , Yan Ruiling , Yang Xuesong , Li Ruiman , Wang Guang
Published: May 2024

BACKGROUND:

Preeclampsia is a significant pregnancy disorder with an unknown cause, mainly attributed to impaired spiral arterial remodeling.

METHODS:

Using RNA sequencing, we identified key genes in placental tissues from healthy individuals and preeclampsia patients. Placenta and plasma samples from pregnant women were collected to detect the expression of TPBG (trophoblast glycoprotein). Pregnant rats were injected with TPBG-carrying adenovirus to detect preeclamptic features. HTR-8/SVneo cells transfected with a TPBG overexpression lentiviral vector were used in cell function experiments. The downstream molecular mechanisms of TPBG were explored using RNA sequencing and single-cell RNA sequencing data. TPBG expression was knocked down in the lipopolysaccharide-induced preeclampsia-like rat model to rescue the preeclampsia features. We also assessed TPBG’s potential as an early preeclampsia predictor using clinical plasma samples.

RESULTS:

TPBG emerged as a crucial differentially expressed gene, expressed specifically in syncytiotrophoblasts and extravillous trophoblasts. Subsequently, we established a rat model with preeclampsia-like phenotypes by intravenously injecting TPBG-expressing adenoviruses, observing impaired spiral arterial remodeling, thus indicating a causal correlation between TPBG overexpression and preeclampsia. Studies with HTR-8/SVneo cells, chorionic villous explants, and transwell assays showed TPBG overexpression disrupts trophoblast/extravillous trophoblast migration/invasion and chemotaxis. Notably, TPBG knockdown alleviated the lipopolysaccharide-induced preeclampsia-like rat model. We enhanced preeclampsia risk prediction in early gestation by combining TPBG expression with established clinical predictors.

CONCLUSIONS:

These findings are the first to show that TPBG overexpression contributes to preeclampsia development by affecting uterine spiral artery remodeling. We propose TPBG levels in maternal blood as a predictor of preeclampsia risk. The proposed mechanism by which TPBG overexpression contributes to the occurrence of preeclampsia via its disruptive effect on trophoblast and extravillous trophoblast migration/invasion on uterine spiral artery remodeling, thereby increasing the risk of preeclampsia.
Tenecteplase Versus Alteplase in Acute Ischemic Stroke in Chinese Patients: Protocol for the ORIGINAL Study
Authors: Meng Xia , Li Shuya , Dai Hongguo , Lu Guozhi , Wang Weiwei , Che Fengyuan , Geng Yu , Sun Minghui , Li Xiyan , Wang Yongjun
Published: May 2024

Background

Tenecteplase, a bioengineered variant of alteplase, is a new alternative thrombolytic agent. The ORIGINAL study aims to evaluate the efficacy and safety of tenecteplase versus standard care in Chinese patients with acute ischemic stroke within 4.5 hours of symptom onset.

Methods

This is a multicenter phase III study that employs a randomized (1:1), active‐controlled, parallel‐group, prospective, open‐label, blinded–end point design. Adult participants (aged ≥18 years) with acute ischemic stroke who are eligible for intravenous thrombolysis within 4.5 hours of symptom onset according to current guideline recommendations are recruited from ≈55 neurology clinics/stroke centers throughout China.

Results

The primary objective of the ORIGINAL study is to demonstrate the noninferiority of tenecteplase (0.25 mg/kg) to alteplase (0.9 mg/kg) on the basis of the proportion of patients who achieve a modified Rankin Scale score of 0 or 1 on day 90, that is, a favorable functional outcome. The prespecified noninferiority risk ratio margin is 0.937. Secondary end points include other functional outcomes and the following safety end points: adjudicated symptomatic intracerebral hemorrhage (up to 36 hours after the end of study drug administration) based on the European Cooperative Acute Stroke Study III definition; all‐cause death within 90 days; and the proportion of patients with a modified Rankin Scale score of 5 or 6 on day 90.

Conclusions

It is anticipated that the results of this study will contribute to the growing body of evidence for the noninferiority of tenecteplase to alteplase given within 4.5 hours of acute ischemic stroke symptom onset and support a new indication for tenecteplase in China.

Clinical Trial Registration Information

NCT04915729 (
https://clinicaltrials.gov/study/NCT04915729
)
Edible insects as a sustainable protein source: a meta-analysis
Authors: Nasir S.Q. , Palupi E. , Nasution Z. , Ploeger A. , Susanto I. , Setiawan B. , Rimbawan R. , Jayanegara A.
Published: May 2024

With the global population set to reach 9.7 billion by 2050, posing a potential 75% increase in food demand, this study examined the viability of edible insects as a sustainable protein source compared with beef. Employing a meta-analysis approach, data were synthesized from studies conducted over the past 13 years, using Hedges’ d effect size and mixed model methods. The parameter values included the nutritional, environmental, and economic aspects of edible insects. Out of 10,119 articles screened, 222 were selected for analysis, revealing 135 different species. Subsequently, 10 species were selected, based on the most comprehensive data available, for mixed model analysis. Although the protein content of these 10 edible insect species was generally lower than that of beef, certain species exhibited amino acid scores surpassing those of beef. Edible insects also exhibited significantly higher calcium, iron, and zinc content compared with beef. Environmental aspects may enhance the value of edible insects as they exhibited greater advantages compared with beef. Despite some identified risks in incorporating edible insects into the diets of humans and animals, there remain potential areas for further investigation and development, such as addressing fat content, indigestible carbohydrate, availability, and sustainability aspects. Further research is required to promote local edible insect products as nutritious food and feed.
Effect of Turbulence on the Collision Rate between Settling Ice Crystals and Droplets
Authors: Sheikh M. Z. , Gustavsson K. , Lévêque E. , Mehlig B. , Pumir A. , Naso A.
Published: May 2024

In mixed-phase clouds, graupel forms by riming, a process whereby ice crystals and supercooled water droplets settling through a turbulent flow collide and aggregate. We consider here the early stage of the collision process of small ice crystals with water droplets and determine numerically the geometric collision kernel in turbulent flows (therefore neglecting all interactions between the particles and assuming a collision efficiency equal to unity), over a range of energy dissipation rate 1–250 cm2 s−3 relevant to cloud microphysics. We take into account the effect of small, but nonzero fluid inertia, which is essential since it favors a biased orientation of the crystals with their broad side down. Since water droplets and ice crystals have different masses and shapes, they generally settle with different velocities. Turbulence does not play any significant role on the collision kernel when the difference between the settling velocities of the two sets of particles is larger than a few millimeters per second. The situation is completely different when the settling speeds of droplets and crystals are comparable, in which case turbulence is the main cause of collisions. Our results are compatible with those of recent experiments according to which turbulence does not clearly increase the growth rate of tethered graupel in a flow transporting water droplets.