bluuebunny/crossref_metadata_2025 huggingface dataset:
FIGURE 4 from WNT4 Regulates Cellular Metabolism via Intracellular Activity at the Mitochondria in Breast and Gynecologic Cancers
Authors: Sottnik Joseph L. , Shackleford Madeleine T. , Robinson Sydney K. , Villagomez Fabian R. , Bahnassy Shaymaa , Oesterreich Steffi , Hu Junxiao , Madak-Erdogan Zeynep , Riggins Rebecca B. , Corr Bradley R. , Cook Linda S. , Treviño Lindsey S. , Bitler Benjamin G. , Sikora Matthew J.
Published: January 2024

WNT4 knockdown impairs respiration but not glycolysis. A, Seahorse MitoStress test in MM134. Points represent mean of 6 biological replicates ± SD. B, Basal respiration is reduced by ER or WNT4 knockdown, but WNT4 knockdown impairs respiratory capacity. C, WNT4 knockdown suppresses OCR, that is, respiration (from B), but has a minimal effect on ECAR, that is, glycolysis. D, From metabolomics data, cellular lactic acid levels are reduced by ER knockdown, but not WNT4 knockdown. B–D, comparisons by ANOVA with Dunnett correction. *, adj.P < 0.05. E, Reduction in lactic acid production was also observed in lactate levels in conditioned medium. *, Two-way ANOVA siRNA effect P < 0.05. F and G, DEMETER2 scores for n = 15 genes in pyruvate metabolism [Kyoto Encyclopedia of Genes and Genomes (KEGG) M00001 and M00307] normalized as Z-scores for ER+ breast cancer cell lines. DEMETER2 siRNA used as ER+ ILC cell line data are not available in Depmap CRISPR-based screens. CAMA1 denoted separately as an “ILC-like” cell line. F, Total sum of Z-scores for the geneset per cell line. G, Hierarchal clustering for gene Z-scores indicates ER+ ILC lines as an independent cluster. Pyruvate metabolism genes at left, representative TCA genes at right.
FIGURE 3 from WNT4 Regulates Cellular Metabolism via Intracellular Activity at the Mitochondria in Breast and Gynecologic Cancers
Authors: Sottnik Joseph L. , Shackleford Madeleine T. , Robinson Sydney K. , Villagomez Fabian R. , Bahnassy Shaymaa , Oesterreich Steffi , Hu Junxiao , Madak-Erdogan Zeynep , Riggins Rebecca B. , Corr Bradley R. , Cook Linda S. , Treviño Lindsey S. , Bitler Benjamin G. , Sikora Matthew J.
Published: January 2024

Metabolic effects of WNT4 knockdown mirror ER knockdown but have expanded impact on fatty acid and amino acid metabolic pathways. A, Metabolite levels altered by WNT4 knockdown (n = 77); pink text indicates a shared affected metabolite with ER knockdown. B, Metabolites dysregulated by WNT4 versus ER knockdown are strongly enriched for overlap. C, WNT4 versus ER knockdown effects on metabolite levels are strongly directly correlated, suggesting an overall parallel effect on ILC cell metabolism. D, Pathway analysis with ER metabolites (C) and WNT4 metabolites (Fig. 4A); P < 0.4 in ER and/or WNT4 dataset shown.
Data from WNT4 Regulates Cellular Metabolism via Intracellular Activity at the Mitochondria in Breast and Gynecologic Cancers
Authors: Sottnik Joseph L. , Shackleford Madeleine T. , Robinson Sydney K. , Villagomez Fabian R. , Bahnassy Shaymaa , Oesterreich Steffi , Hu Junxiao , Madak-Erdogan Zeynep , Riggins Rebecca B. , Corr Bradley R. , Cook Linda S. , Treviño Lindsey S. , Bitler Benjamin G. , Sikora Matthew J.
Published: January 2024

Abstract
Wnt ligand WNT4 is critical in female reproductive tissue development, with WNT4 dysregulation linked to related pathologies including breast cancer (invasive lobular carcinoma, ILC) and gynecologic cancers. WNT4 signaling in these contexts is distinct from canonical Wnt signaling yet inadequately understood. We previously identified atypical intracellular activity of WNT4 (independent of Wnt secretion) regulating mitochondrial function, and herein examine intracellular functions of WNT4. We further examine how convergent mechanisms of WNT4 dysregulation impact cancer metabolism. In ILC, WNT4 is co-opted by estrogen receptor α (ER) via genomic binding in WNT4 intron 1, while in gynecologic cancers, a common genetic polymorphism (rs3820282) at this ER binding site alters WNT4 regulation. Using proximity biotinylation (BioID), we show canonical Wnt ligand WNT3A is trafficked for secretion, but WNT4 is localized to the cytosol and mitochondria. We identified DHRS2, mTOR, and STAT1 as putative WNT4 cytosolic/mitochondrial signaling partners. Whole metabolite profiling, and integrated transcriptomic data, support that WNT4 mediates metabolic reprogramming via fatty acid and amino acid metabolism. Furthermore, ovarian cancer cell lines with rs3820282 variant genotype are WNT4 dependent and have active WNT4 metabolic signaling. In protein array analyses of a cohort of 103 human gynecologic tumors enriched for patient diversity, germline rs3820282 genotype is associated with metabolic remodeling. Variant genotype tumors show increased AMPK activation and downstream signaling, with the highest AMPK signaling activity in variant genotype tumors from non-White patients. Taken together, atypical intracellular WNT4 signaling, in part via genetic dysregulation, regulates the distinct metabolic phenotypes of ILC and gynecologic cancers.
Significance:
WNT4 regulates breast and gynecologic cancer metabolism via a previously unappreciated intracellular signaling mechanism at the mitochondria, with WNT4 mediating metabolic remodeling. Understanding WNT4 dysregulation by estrogen and genetic polymorphism offers new opportunities for defining tumor biology, precision therapeutics, and personalized cancer risk assessment.
Nail Squamous Cell Papilloma: A Rare Case Report
Authors: Macagnan Maiara Christine , Meneguetti Maria Elisa , Gioia Di Chiacchio Nilton , Matter Adriana , Ferrari Thiago Augusto
Published: January 2024

Introduction: Squamous cell papilloma is a benign tumor whose pathogenesis is generally related to the human papillomavirus. Despite affecting several organs, we did not find cases reported in the nails. Case Presentation: A 67-year-old female presented with a painful lesion in the nail of the right hallux that started in 2021, with an erythematous appearance evolving to black and expansive growth. Due to the evolution and symptoms, she opted for excision, with a histopathological report of squamous cell papilloma. Complementary treatment with imiquimod was performed, with good healing and pain control. Discussion: Due to the lack of previous reports, treatment was based on therapeutic options for papillomas located in other regions. Excision was performed, followed by application of imiquimod with good response. The prognosis is favorable; however, further studies are needed to elucidate the diagnosis and management of nail squamous cell papilloma.
A ruptura paradigmática da comunicação
Authors: Sodré Muniz
Published: January 2024

This paper is focused on discussing the historical emergence of communication as a practice and theory of an anthropological reconfiguration by means of disruptive technologies and episteme radically opposed to late 18th century mechanistic and positivist social thought systems. Autopoiesis. Ancient original wisdom. Common good. Measures of embracing artificial intelligence. Epistemological rupture and a proposal for communication as a science of the commons.
La internacionalización de la investigación en comunicación: algunas notas críticas y una propuesta
Authors: Porta Eva Da
Published: January 2024

Este artículo analiza las tendencias generales en torno a los procesos de internacionalización de la ciencia que se están desarrollando en las universidades y centros de investigación de América Latina y el Caribe, y se detiene en el estudio de algunas características del campo académico de la comunicación. Luego, revisa críticamente la configuración discursiva en la que se inscriben los procesos de internacionalización de la ciencia con el propósito de resignificar y poner en cuestión algunos procesos de legitimación del conocimiento que siguen modelos no adecuados para el campo de la investigación en comunicación. Posteriormente, sobre el cierre propone realizar un mapeo de los estudios de comunicación en la región que recupere la noción de mapa nocturno de Martín-Barbero para diseñar una cartografía colaborativa entre equipos de investigación articulados como nodos.
¿Cómo enfrentar las desigualdades de la academia global en los estudios de comunicación?: colaboración, crítica y curiosidad
Authors: Waisbord Silvio
Published: January 2024

Hay profundas desigualdades y hegemonía de la academia del Norte Global ejemplificadas por publicaciones, referencias y cuerpos editoriales de revistas, y el lugar preponderante del inglés como lingua franca de la academia global. Esto contribuye a la persistente marginalización e invisibilidad de académicos y “estudios del Sur”. Frente a esta situación, este trabajo plantea tres estrategias: Consolidar espacios de reconocimiento y apoyo; Cultivar redes de colaboración e investigación con perspectivas comparativas e integradoras; y Participar en espacios compartidos.
Adeus à crítica?: passado e presente da teoria e método na pesquisa em comunicação de massa
Authors: Rüdiger Francisco
Published: January 2024

Critical research in mass communication has progressed theoretical and practically in several areas of knowledge: political economy, cultural studies, analytical social psychology, social history, discourse analysis, etc., in the wake of what has been presented as a new form of science social, the critical, under the influence of cultural Marxism and, by this way, of the “critical theories”. This article rationally reconstructs central aspects of the development of this problematics, aiming to show the constitutive impasses that result from the interference of a very rigid and dogmatic theory, currently degraded, in a scientific research program that, it is believed, has not yet exhausted its epistemic and reflexive potential.
Carta para Joaquim José da Cruz
Authors: Pereira Gabriela Acerbi
Published: January 2024

Este ensaio é composto por uma carta escrita pela autora e por três colagens feitas a partir do acervo pessoal de Joaquim José da Cruz, o último Moçambique que viveu na cidade de Poços de Caldas, no Sul de Minas Gerais. Ele dançou nos festejos para São Benedito, Nossa Senhora do Rosário e Santa Ifigênia por mais de sessenta anos. Poucos meses antes da sua partida, ele desejou ter suas imagens e seu acervo pessoal passados a diante. Esse ensaio faz parte da realização desse pedido.
Autoridade negativa
Authors: Graeber David , Soares Renato Martelli , Braga Leonardo Viana
Published: January 2024

Quais são as formas legítimas de autoridade em Madagascar? Segundo Graeber os anciãos e ancestrais são vistos como autoridade legítima porque não seriam vistos como reis (que mandam) e intervêm na vida dos outros dizendo o que não fazer –não o que fazer. Um sistema de "autoridade negativa" repleto de ambivalências que para ser entendido deve ser visto à luz do sistema de parentesco merina.
Opressão
Authors: Graeber David , Soares Renato Martelli , Braga Leonardo Viana
Published: January 2024

As conexões linguísticas entre a palavra "opressão" e a sensação "ser prensado, esmagado por um peso pesado” que existe em quase todas as línguas, apontam para uma reflexão sobre os dilemas políticos que surgem do relativismo cultural.
Magia do amor e moralidade política no centro de Madagascar, 1875-1990
Authors: Graeber David , Soares Renato Martelli , Braga Leonardo Viana
Published: January 2024

Ensaio sobre a "medicina do amor", sobre como ela foi inicialmente atribuída a usuárias femeninas e depois a usuários masculinos e como seu uso e significado estão vinculados às mudanças que trouxe a ocupação colonial francesa em Madagascar.
Cidades-estado arbóreas: fitopolíticas da guerra no imaginário urbano urarina
Authors: Fabiano Emanuele
Published: January 2024

No imaginário urbano urarina, uma extensa rede de metrópoles ocupa a floresta tropical. Essas cidades dendríticas têm características fortemente normativas e até mesmo opressivas, inspiradas em modelos sociopolíticos hierárquicos e autoritários, que definem a relação entre as diferentes espécies de árvores e seus vizinhos humanos. Produção, tecnologia e comércio orientam os governos dessas enormes cidades-estado, lugares conhecidos por sua produtividade e eficiência, nos quais o cultivo intensivo, a criação de gado em larga escala e a fabricação de artefatos industriais sustentam uma ampla fito-política de guerra. Meu artigo analisa como a topografia e a morfologia social dessas cidades-estado reproduzem, em um plano cosmológico, as formas de dominação e subordinação vivenciadas hoje pelos Urarina na Amazônia peruana. Além disso, discuto como a produção - e a constante atualização - desse imaginário urbano indígena serve para desnaturalizar os efeitos das relações com a sociedade nacional estratificada e tecnológica.
As sátiras de Ênio: tradução e comentário
Authors: Aubert Eduardo Henrik
Published: January 2024

Este texto apresenta a primeira tradução para o português das sátiras do poeta republicano Quinto Ênio (c. 239-169 a.C.), transmitidas de forma fragmentária e sobre as quais pairam dúvidas de múltiplas ordens. Na introdução, procura-se identificar distintas correntes interpretativas, de que resultam percepções bastante distintas desses fragmentos. Na sequência, fragmentos e seus contextos vêm traduzidos, acompanhados de comentário destinado a esclarecer sobretudo as relações intra e inter-genéricas que contribuíram para a composição de tais poemas.
Teorias do corpo para pensar o social
Authors: Ennes Marcelo Alario , Jesus Bruno Henrique Souza de
Published: January 2024

Neste artigo procuramos refletir sobre a importância do corpo para a análise do social. Para tanto, apoiamo-nos em quatro autores frequentemente referenciados na produção bibliográfica recente em que o corpo é acionado como categoria analítica ou dimensão empírica do estudo, a saber: David Le Breton, Michael Foucault, Judith Butler e Pierre Bourdieu. Nosso objetivo era identificar a importância do corpo para cada um destes autores. De um modo geral, verificamos que para todos eles o corpo está intimamente imbricado com o poder, seja como expressão de identidades e do existencialismo, Le Breton; como materialização do poder, Foucault; como linguagem, Butler ou, ainda, como objetivação do social, Bourdieu.
Classe descentralizada da ETEC: palco de (re)existência, resistência e resiliência
Authors: Calsan Eduardo , Contro Soraya Kullerkupp
Published: January 2024

Políticas públicas e políticas de governo coexistem conflituosamente no campo da educação: enquanto a primeira reflete uma falsa homogeneidade, tornando-se franca e descontínua, a outra se impõe de acordo com a vontade do grupo hegemônico da vez. Este presente estudo possui a pretensão de identificar uma política de governo exitosa, no campo da educação, detidamente na educação técnica e demonstrar, através de índices quantitativos e características qualitativas, os desafios e obstáculos impostos às classes descentralizadas ligadas às Etecs, mostrando que esse espaço pedagógico é palco de (re)existência, resistência e resiliência.
Entrevista com a profa. Dra. Marilda Aparecida de Menezes
Authors: Magalhães Valéria Barbosa de
Published: January 2024

“A história oral é uma metodologia de uma riqueza e uma potencialidade enormes, que se combina com uma perspectiva de migrações centrada nos sujeitos sociais, centrada nos migrantes e não nos processos”

A entrevista foi realizada nos dias 28 de fevereiro e 13 de agosto de 2019, por Valéria Barbosa de Magalhães, no âmbito do projeto de pesquisa Nordestinos em São Paulo e História Oral: Abordagem histórico-crítica, com vigência 2017 a 2020, financiado pela Fapesp e coordenado por Valéria B. Magalhães. Transcrição: Aline Campos dos Reis (bolsista TT Fapesp) e Andréa Moreira Santana (bolsista PUB/USP). Conferência: Valéria B. Magalhães.
Humanitarian Public Diplomacy in Comparative Perspective: The 2015 Refugee Crisis (2015-2021), the Syrian Civil War (2011-2022) and the 2015 Nepal Earthquake
Authors: Dingott Alkopher Tal , Barak Naama
Published: January 2024

The aim of the article is to enhance understanding of the phenomenon of humanitarian public diplomacy in relation to digital communication strategics. It aspires to grasp the nature of discursive practices and strategies of public communications used by practitioners of humanitarian diplomacy. The article analytically maps discursive practices and strategies of public communications employed by humanitarian international organisations across humanitarian crisis cases and explains similarities and differences across cases. Three contexts of humanitarian crisis were chosen as case studies: the humanitarian crisis resulting from the war in Syria (2011-2022); the refugee-related humanitarian crisis in the Mediterranean (2015-2021); and the humanitarian crisis that accompanied the Nepal earthquake in 2015.
Bee Movie
Authors: Denton Andrew , Gibbons Andrew
Published: January 2024

Bee Movie is a short film that invites questions for conversations on visual ethics. The viewer is invited into a world of circular, tragic and absurd questions concerning what a filmmaker, an abstract writer, a journal editor, and a film viewer ought to do when observing the apparent reality of a bee’s circular attempts to escape a pond. As a filmmaker and abstract writer, one does not want to tell the viewer and reader how one feels about this bee, bees, insects, ponds, water, life, death and circles. And one feels obliged not to explain the context through which the film came to life as the bee was engaged in efforts that might be narrated as lifesaving but also as another complex of efforts entirely. As an open image, without our textual dissection (or, at least, with a dampening of that dissection to an abstract and with a few questions and challenges), we regard Bee Movie as inviting questions about the ethics of the use of images as pedagogy. Whatever we thought about whether and how to share this film, we find ourselves in 359 other relationships, and always back again to what we assumed might be the starting position.
What Visibilising Pedagogical Approaches do the Chinese as a Foreign Language (cfl) Teachers Adopt in the Teaching of Chinese Characters?
Authors: Yu Jinghan , Fletcher Jo , Everatt John , Astall Chris
Published: January 2024

Chinese can be considered as one of the more challenging languages to learn for many non-native learners. Its complicated visual script, the Chinese characters, demands that teachers be proficient in utilizing diverse visual pedagogical approaches, coupled with different digital visual tools, to enhance their teaching effectiveness. Adopting a descriptive qualitative analysis method, interview transcripts from fourteen cfl teachers were examined. There were five online teachers, four university teachers and five pre-service teachers. The findings revealed that: 1) all the teachers use various visual pedagogical approaches, such as pictures, videos, Chinese character digital image files, Chinese character studying applications, and websites, to enhance the effectiveness of Chinese character learning, as well as motivate and engage learners; 2) teachers choose different pedagogical approaches to help students visualise characters as a whole unit or as several separate radical components; 3) the use of Pinyin to input characters on digital devices has been widely taught in class; and 4) online teachers, and some pre-service teachers, have replaced traditional handwriting methods with digital character input methods, whereas university teachers have not made this shift. The ways in which these perspectives relate to the form of Chinese characters and developing teaching practices are discussed.
Agaricus section Minores: New and noteworthy species from India
Authors: ARYA C.P. , PRADEEP C.K.
Published: January 2024

In this study, four species of Agaricus sect. Minores are presented based on morphological and nrITS based phylogenetic analyses (Maximum Likelihood and Bayesian Inference). The molecular analysis and morphological characteristics supported the placement of these species in A. sect. Minores. Three species, viz., Agaricus indicus, A. palodensis, and A. wayanadensis are new to science, while A. purpureofibrillosus is a new phytogeographical record. Comprehensive descriptions, photographs, phylogenetic analyses results, and comparisons with related species are provided.
Application Effect of Bacillus in Tomato Root Knot Nematode Disease Control
Authors: Geng Yunhong
Published: January 2024

Root-knot nematode is a kind of omnivorous plant pathogenic nematode that causes great harm to underground roots and above-ground parts of plants, and has become a serious factor restricting the development of global agriculture. In order to realize the sustainable development of agriculture, the research carried out an in-depth discussion on biological control methods. Firstly, a bacillus was obtained through insecticidal experiments, then a large amount of materials was obtained through purification and cultivation, and then the stability test of the active substance of Bacillus was conducted to explore its influence to root-knot nematodes. Finally, the pot verification experiment and field verification experiment were conducted. The results showed that in the insecticidal experiment, the accumulation of active substances at 14 h was the maximum, and the corresponding mortality of root-knot nematodes at 12 h and 24 h was 97.6% and 82.91%, respectively. In the BS pot experiment, the number of tomato root knot formation in the bacillus treated group was significantly reduced by 46.3% compared with the control group, and the number of egg sac of the rhizome was reduced by 78.31%. On the 120th day of field verification experiment, the fruit quantity and yield per plant in the treatment group increased by 17.02% and 11.85%, respectively. In summary, Bacillus can effectively reduce the damage of root knot nematode to tomato plants, and has a good prospect of application and popularization.
Estimation of the Smallest Acceptable Sample Size in Bilateral Approaches to Coefficient Estimation and Accuracy Prediction
Authors: Cai Bowen , Wang Xuesong
Published: January 2024

This study is about smallest acceptable sample size determination in experimental design studies involving a driving simulator. The smallest acceptable sample size should be specified so researchers can make accurate inferences about their studied populations. However, the number of samples typically collected is largely subject to the expense of data collection. Working out the methodology of estimating the required number of subjects based on an initially small number is a better way for researchers to determine the smallest acceptable sample size in the experiment. Predictor estimate precision and prediction accuracy are major factors for conducting experiments. Accordingly, this study estimates the smallest acceptable sample size, with emphasis on coefficient estimation and prediction accuracy for selected significant variables. The smallest acceptable sample size is chosen to be the maximum value returned by both coefficient estimation calculation and accuracy prediction calculation approaches. This methodology is flexible and scalable, and can be tailored to other experimental situations. To validate the appropriateness of this procedure, a more than sufficient sample of 50 drivers was recruited. The smallest acceptable sample size was determined backwardly, based on the variable coefficient convergence trends of the mean squared error (MSE) curves of the significant variables. Both the clear converging trends of the MSE curves and the proposed method indicated that 30 was an acceptable sample size.
Improved sliding mode extension control of vehicle active front wheel steering based on extended state observer
Authors: Huang Yuang , Zhao Youqun , Wang Junzhu , Lin Fen
Published: January 2024

In order to improve the handling stability of electric vehicles, a new active front-wheel steering (AFS) control method was proposed. Firstly, parametric uncertainty and external interference in vehicle dynamics are summarized as a nonlinear interference term in vehicle model. An extended state observer (ESO) is designed to observe and compensate the nonlinear interference terms in real time, so as to improve the accuracy and control effect of the model. Secondly, in order to further increase the convergence speed and effectively suppress the chattering phenomenon in sliding mode control without affecting its robustness and reaching speed, an integral exponential fast terminal sliding mode controller (IEFTSMC) based on fast exponential reaching law (FERL) is designed. The problem that the control effect of some regions becomes worse when a single control algorithm is used for global region control in traditional AFS control is addressed. By combining extension theory with sliding mode control method, an improved sliding mode extension control is designed to improve the effect of AFS global control. Finally, comparative simulation tests are carried out on the CarSim/Simulink co-simulation platform. The results show that compared with the traditional FTSMC, the improved sliding mode extension control method based on ESO can not only suppress chattering more effectively, but also smoother the response curve. It also has good control effect when there is external disturbance. The effectiveness and robustness of the control strategy are verified.
Combustion synthesis, powder treatment, dispersion and tape casting of lanthanum strontium manganite
Authors: Kakade M.B. , Ramanathan S. , De P.K.
Published: January 2024

Lanthanum strontium manganite La0·84Sr0·16MnO3(LSM)powder was prepared by combustion synthesis, using a concentrated solution of nitrates of lanthanum, strontium, and manganese as oxidiser, and citric acid as fuel. The as formed powder was found to be crystalline LSM consisting of porous agglomerates of fine particles, and exhibited about 7% total weight loss on heating up to 1100 °C. Dispersion conditions for wet grinding of the powder agglomerates calcined at 1100 °C were evaluated from zeta potential and viscositystudies. A maximum zeta potential of - 35 mV was noted at a pH of 11, while pseudoplasticity of the slurry decreased with decreasing solid concentration. Powder with d50of around 3 μm and free of agglomerates of size greater than 10 μm was formed by wet grinding. Further calci nation of this powder at 1350 °C improved the solids loading in the slurry. The tape cast slurry composition was optimised for minimum content of binder, plasticiser, and water with acceptable pseudoplasticity to form flexible and flat tapes. The dried tapes cut to required sizes were subjected to controlled binder burnout followed by sintering in the range 1350-1450 °C. The sintered flat specimens exhibited densities in the range 65-80% of theoretical, and open porosities in the range 35-20%, with a homogeneous distribution of pore phase in the matrix.
Impact of cucurbit crop management techniques on the foraging behavior of honeybees and hoverflies in Morogoro, Tanzania
Authors: Rweyemamu Elvillah William , Mwatawala Maulid Walad , Tryphone George Muhamba , De Meyer Marc , Kabota Sija , Bwire Patroba Masatu
Published: January 2024

Background

Poor agricultural practices have drastically threatened insect pollinators’ biodiversity. Little is known in Tanzania about how different agricultural practices affect pollinators’ foraging behavior. This study investigated the effects of the agroecological zone, season, cucurbit species and management practices on visitation frequency, visitation rate and time spent on cucurbit flowers by five pollinator species viz. Apis mellifera, Eristalinus megacephalus, Mesembrius caffer, Paragus borbonicus and Toxomerus floralis. The experiment was designed as a 5 × 3 × 3 × 2 × 2 factorial arrangement in a Randomized Complete Block Design (RCBD) with four replications. GAMOUR-Agroecology was tested against conventional practices and untreated control.

Results

This study revealed significant effects of agroecological zone × season × cucurbit species × management practice on pollinators’ visitation frequency (p = 0.007) and time spent on flowers (p = 0.005). Also, agroecological zone × season × cucurbit species × pollinator species significantly (p < 0.0001) affected pollinators’ visitation frequency. Agroecological zones × season × cucurbit species × cucurbits management practices × pollinators significantly (p = 0.001) affected pollinators’ visitation rate. Apis mellifera was the most frequent visitor in Cucurbita moschata plots treated with GAMOUR- Agroecology in the plateau zone, also, visited higher number of Cucumis sativus plots under GAMOUR-Agroecology practices in the mountainous zone during the October–November season. Furthermore, it has been found that pollinators spent much in cucurbit flowers on plots with GAMOUR-Agroecology practices and control.

Conclusions

Pollinators’ foraging behavior were enhanced by GAMOUR-Agroecology practices. Therefore, this study recommended that cucurbit growers should consider management practices that positively influence pollinator foraging activities for sustainable cucurbit production.
Genomic data integration tutorial, a plant case study
Authors: Mardoc Emile , Sow Mamadou Dia , Déjean Sébastien , Salse Jérôme
Published: January 2024

Background

The ongoing evolution of the Next Generation Sequencing (NGS) technologies has led to the production of genomic data on a massive scale. While tools for genomic data integration and analysis are becoming increasingly available, the conceptual and analytical complexities still represent a great challenge in many biological contexts.

Results

To address this issue, we describe a six-steps tutorial for the best practices in genomic data integration, consisting of (1) designing a data matrix; (2) formulating a specific biological question toward data description, selection and prediction; (3) selecting a tool adapted to the targeted questions; (4) preprocessing of the data; (5) conducting preliminary analysis, and finally (6) executing genomic data integration.

Conclusion

The tutorial has been tested and demonstrated on publicly available genomic data generated from poplar (Populus L.), a woody plant model. We also developed a new graphical output for the unsupervised multi-block analysis, cimDiablo_v2, available at https://forgemia.inra.fr/umr-gdec/omics-integration-on-poplar, and allowing the selection of master drivers in genomic data variation and interplay.
Effects of propofol versus sevoflurane on surgical field visibility during arthroscopic rotator cuff repair: a randomized trial
Authors: Yin Wenchao , Yin Chenzhu , Wang Wencan , Peng Rao , Su Li , Li Peiyu
Published: January 2024

Background

During arthroscopic rotator cuff repair (ARCR), clear surgical field visibility (SFV) is the basis of successful surgery, but the choice of anesthesia maintenance drugs may have different effects on SFV. In this study, we aimed to compare the effects of propofol- and sevoflurane-based general anesthesia on SFV in patients undergoing ARCR.

Methods

Patients (n = 130) undergoing elective ARCR in the lateral decubitus position were randomized into either the propofol group or sevoflurane group (65 per group). The duration of surgery and increased pressure irrigation (IPI), Boezaart score, rocuronium consumption and usage of remifentanil were recorded. The time of both spontaneous respiration recovery and extubation and the incidences of postoperative nausea and vomiting and agitation were also recorded.

Results

The Boezaart score, duration of IPI and ratio of the duration of IPI to the duration of surgery (IPI/S ratio) were similar between the groups (P > 0.05). Rocuronium consumption, number of patients requiring remifentanil infusion and total remifentanil consumption were significantly lower in the sevoflurane group (P < 0.05). The spontaneous respiration recovery time was significantly longer in the propofol group (P < 0.05), but there were no differences in the extubation time between the groups(P > 0.05).

Conclusions

Compared with propofol, sevoflurane provides equally clear SFV while improving the convenience of anesthesia maintenance in ARCR patients with interscalene plexus (ISB) combined with general anesthesia.

Trial registration

This single-center, prospective, RCT was retrospective registered at Chinese Clinical Trial Registry with the registration number ChiCTR2300072110 (02/06/2023).
Heterogeneity by age and gender in the association of kidney function with mortality among patients with diabetes - analysis of diabetes registry in Singapore
Authors: Jafar Tazeen H. , Seng Loraine Liping , Wang Yeli , Lim Ching Wee , Chan Choong Meng , Kwek Jia Liang , Coffman Thomas M. , Ping Yongjing , Bee Yong Mong , Allen John C.
Published: January 2024

Background

We aimed to explore the three-way interaction among age, gender, and kidney function on the risk of all-cause mortality and cardiovascular mortality among patients with type 2 diabetes (T2D).

Methods

In a retrospective cohort study, patients aged > 40 years with T2D with serum creatinine and urine albumin measured from 2013 to 2019 were included from a multi-institutional diabetes registry. The exposure was estimated glomerular filtration rate (eGFR), outcomes were all-cause mortality (primary outcome) and cardiovascular disease (CVD) mortality (secondary outcome). We applied multivariable cox proportional hazards regression analysis to compute the association between eGFR and mortality.

Results

A total of 36,556 patients were followed for up to 6 years during which 2492 (6.82%) died from all causes, and 690 (1.9%) died from CVD. We observed a significant three-way interaction (p = 0.021) among age (younger, < 65; older, ≥65 years), gender and eGFR for the risk of all-cause mortality. Using age- and gender-specific eGFR of 90 ml/min/1.73m2 as the reference point, the adjusted hazard rate (HR) (95% CI) for all-cause mortality at eGFR of 40 ml/min/1.73m2 was 3.70 (2.29 to 5.99) in younger women and 1.86 (1.08 to 3.19) in younger men. The corresponding adjusted HRs in older women and older men were 2.38 (2.02 to 2.82) and 2.18 (1.85 to 2.57), respectively. Similar results were observed for CVD deaths, although the three-way interaction was not statistically significant. Sensitivity analysis yielded similar results.

Conclusions

In this T2D population, younger women with reduced kidney function might be more susceptible to higher risks of all-cause mortality and CVD mortality than younger men.
The comparison of pure uterine serous carcinoma and mixed tumor with serous component: a single-institution review of 91 cases
Authors: You Xuewu , Dong Yangyang , Wang Jiaqi , Cheng Yuan , Jia Yuanyuan , Zhang Xiaobo , Wang Jianliu
Published: January 2024

Background

Pure uterine serous carcinoma (p-USC) and mixed tumors with serous component (m-USC) are aggressive subtypes of endometrial cancer associated with high mortality rates. This retrospective study aimed to compare clinicopathologic features and outcomes of p-USC and m-USC in a single center.

Methods

This study retrospectively reviewed patients diagnosed with USC at Peking University People’s Hospital between 2008 and 2022. T-tests and chi-square tests were used to compare clinicopathological characteristics between p-USC and m-USC. Kaplan-Meier survival curve and Cox regression analysis were used to analyze the impact of clinical and pathological variables on OS and PFS.

Results

Among the 91 patients who underwent surgery, 65.9% (n = 60) were p-USC, and 34.1% (n = 31) were m-USC. Patients with p-USC had earlier menopause (P = 0.0217), a lower rate of progesterone receptor(PR) expression (P < 0.001), and were more likely to have positive peritoneal cytology (P = 0.0464). After a median follow-up time of 40 months, 28 (46.7%) p-USC and 9 (29%) m-USC patients had progression disease, 18 (30%) and 8 (25.8%) patients died of their disease. 5-year PFSR were 51.2% and 75.3%, respectively, and 5-year OS rates were 66% and 67.4%. Kaplan-Meier survival analysis showed that p-USC was more likely to relapse than m-USC (P = 0.034), but there was no significant difference in OS. Cox regression analysis showed that lymph node metastasis and surgical approach were risk factors for OS, and myoinvasion depth ≥ 1/2 was an independent risk factor for PFS.

Conclusions

p-USC was more likely to relapse than m-USC, but there was no significant difference in OS between the two subtypes.
EMP3 as a prognostic biomarker correlates with EMT in GBM
Authors: Li Li , Xia Siyu , Zhao Zitong , Deng Lili , Wang Hanbing , Yang Dongbo , Hu Yizhou , Ji Jingjing , Huang Dayong , Xin Tao
Published: January 2024

Background

Glioblastoma (GBM) is the most aggressive malignant central nervous system tumor with a poor prognosis.The malignant transformation of glioma cells via epithelial-mesenchymal transition (EMT) has been observed as a main obstacle for glioblastoma treatment. Epithelial membrane protein 3 (EMP3) is significantly associated with the malignancy of GBM and the prognosis of patients. Therefore, exploring the possible mechanisms by which EMP3 promotes the growth of GBM has important implications for the treatment of GBM.

Methods

We performed enrichment and correlation analysis in 5 single-cell RNA sequencing datasets. Differential expression of EMP3 in gliomas, Kaplan–Meier survival curves, diagnostic accuracy and prognostic prediction were analyzed by bioinformatics in the China Glioma Genome Atlas (CGGA) database and The Cancer Genome Atlas (TCGA) database. EMP3-silenced U87 and U251 cell lines were obtained by transient transfection with siRNA. The effect of EMP3 on glioblastoma proliferation was examined using the CCK-8 assay. Transwell migration assay and wound healing assay were used to assess the effect of EMP3 on glioblastoma migration. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) and western blot were used to detect the mRNA and protein expression levels of EMT-related transcription factors and mesenchymal markers.

Results

EMP3 is a EMT associated gene in multiple types of malignant cancer and in high-grade glioblastoma. EMP3 is enriched in high-grade gliomas and isocitrate dehydrogenase (IDH) wild-type gliomas.EMP3 can be used as a specific biomarker for diagnosing glioma patients. It is also an independent prognostic factor for glioma patients' overall survival (OS). In addition, silencing EMP3 reduces the proliferation and migration of glioblastoma cells. Mechanistically, EMP3 enhances the malignant potential of tumor cells by promoting EMT.

Conclusion

EMP3 promotes the proliferation and migration of GBM cells, and the mechanism may be related to EMP3 promoting the EMT process in GBM; EMP3 may be an independent prognostic factor in GBM.
Dairy product consumption, eating habits, sedentary behaviour and physical activity association with bone mineral density among adolescent boys: a cross-sectional observational study
Authors: Kopiczko Anna , Czapla Michał , Juárez-Vela Raúl , Ross Catherine , Uchmanowicz Bartosz
Published: January 2024

Background

During childhood and adolescence, skeletal microarchitecture and bone mineral density (BMD) undergo significant changes. Peak bone mass is built and its level significantly affects the condition of bones in later years of life. Understanding the modifiable factors that improve bone parameters at an early age is necessary to early prevent osteoporosis. To identify these modifiable factors we analysed the relationship between dairy product consumption, eating habits, sedentary behaviour, and level of physical activity with BMD in 115 young boys (14–17 years).

Methods

Bone parameters were measured by dual energy x-ray absorptiometry using paediatric specific software to compile the data. Dairy product consumption and eating habits were assessed by means of a dietary interview. Sedentary behaviour and physical activity was assessed in a face-to-face interview conducted using the International Physical Activity Questionnaire. Data collection on total physical activity level was performed by collecting information on the number of days and the duration of vigorous and moderate intensity (MVPA) and average daily time spent in sitting (SIT time).

Results

The strongest relationships with BMD in distal part of forearm were found for moderate plus vigorous activity, sit time, and intake of dairy products, intake of calcium, protein, vitamin D, phosphorus from diet. Relationships between BMD, bone mineral content (BMC) in the distal and proximal part of the forearm and PA, sit time and eating parameters were evaluated using the multiple forward stepwise regression. The presented model explained 48–67% (adjusted R2 = 0.48–0.67; p < 0.001) of the variance in bone parameters. The predictor of interactions of three variables: protein intake (g/person/day), vitamin D intake (µg/day) and phosphorus intake (mg/day) was significant for BMD dis (adjusted R2 = 0.59; p < 0.001). The predictor of interactions of two variables: SIT time (h/day) and dairy products (n/day) was significant for BMD prox (adjusted R2 = 0.48; p < 0.001). Furthermore, the predictor of interactions dairy products (n/day), protein intake (g/person/day) and phosphorus intake (mg/day) was significant for BMC prox and dis (adjusted R2 = 0.63–0.67; p < 0.001).

Conclusions

High physical activity and optimal eating habits especially adequate intake of important dietary components for bone health such as calcium, protein, vitamin D and phosphorus affect the mineralization of forearm bones.
A randomized controlled trial of the dissemination of an mHealth intervention for improving health outcomes: the WiseApp for Spanish-speakers living with HIV study protocol
Authors: Olaya Felix , Brin Maeve , Caraballo Pamela Baez , Halpern Mina , Jia Haomiao , Ramírez Sergio Ozoria , Padilla Janeth Juarez , Stonbraker Samantha , Schnall Rebecca
Published: January 2024

Background

While there is no cure for HIV, adherence to antiretroviral therapy can extend the lifespan and improve the quality of life of people with HIV. Despite the global reduction of HIV infection rates in recent years, New York City and La Romana, Dominican Republic, continue to report high infection rates among Latino populations. Many people with HIV remain virally unsuppressed in these geographic hotspots, suggesting a need for additional interventions to overcome medication adherence barriers. Tailored and culturally appropriate mobile health (mHealth) technology can be an engaging way to improve adherence. The primary objective of this trial is to test the effectiveness of an mHealth tool to improve HIV medication adherence among Spanish-speaking people living in New York City and the Dominican Republic.

Methods

The WiseApp study is a two-arm randomized controlled trial among 248 people with HIV across the New York and Dominican Republic sites over the course of 12 months. Participants are randomly assigned to either receive a CleverCap pill bottle that is linked to the WiseApp (intervention) or standard of care (control). All participants complete surveys at baseline, 3-month, 6-month, and 12-month follow-up visits and the study team obtains HIV-1 viral load and CD4 count results through blood draw at each study timepoint.

Discussion

The use of mHealth technologies to improve medication adherence among people with HIV has been implemented in recent years. Although some studies have found improvement in adherence to antiretroviral therapy in the short term, there is limited information about how these interventions improve adherence among Spanish-speaking populations. Disproportionate rates of HIV infection among Latinos in New York City suggest an existing inequitable approach in reaching and treating this population. Due to a lack of mHealth studies with Latino populations, and apps tailored to Spanish-speakers, the WiseApp study will not only demonstrate the effectiveness of this particular mHealth app but will also contribute to the mHealth research community as a whole.

Trial registration

This trial was registered with Clinicaltrials.gov (NCT05398185) on 5/31/2022.
Determinants and spatial factors of anemia in women of reproductive age in Democratic Republic of Congo (drc): a Bayesian multilevel ordinal logistic regression model approach
Authors: Soda Martin Abysina , Hamuli Eugénie Kabali , Batina Salomon Agasa , Kandala Ngianga-Bakwin
Published: January 2024

Background

As a global public health problem, anemia affects more than 400 million women of reproductive age worldwide, mostly in Africa and India. In the DRC, the prevalence of anemia has decreased slightly from 52.9% in 2007, to 46.4% in 2012 and 42.4% in 2019. However, there is considerable regional variation in its distribution. The aim of this study is to determine the factors contributing to anemia in women of reproductive age and to explore its spatial distribution in the DRC.

Methods

Based on the Bayesian Multilevel Spatial Ordinal Logistic Regression Model, we used the 2013 Democratic Republic of Congo Demographic and Health Survey (DHS-DRC II) data to investigate individual and environmental characteristics contributing to the development of anemia in women of reproductive age and the mapping of anemia in terms of residual spatial effects.

Results

Age, pregnancy status, body mass index, education level, current breastfeeding, current marital status, contraceptive and insecticide-treated net use, source of drinking water supply and toilet/latrine use including the province of residence were the factors contributing to anemia in women of reproductive age in DRC. With Global Moran's I = -0.00279, p-value ≥ 0.05, the spatial distribution of anemia in women of reproductive age in DRC results from random spatial processes. Thus, the observed spatial pattern is completely random.

Conclusion

The Bayesian Multilevel Spatial Ordinal Logistic Regression statistical model is able to adjust for risk and spatial factors of anemia in women of reproductive age in DRC highlighting the combined role of individual and environmental factors in the development of anemia in DRC.
Comprehensive evaluation of the impact of workplace exposures on physician-certified sick leave in the general working population
Authors: Sterud Tom , Marti Andrea R , Degerud Eirik
Published: January 2024

Background

Our objective was to quantify the prospective associations between work factors across chemical, physical, mechanical, and psychosocial domains and the onset of medically certified sick leave.

Methods

Eligible respondents were interviewed in 2009, 2013, or 2016 and were registered in the national sick leave register with an employee relationship lasting more than 50 working days during the year of the survey interviews and the following year (n = 15,294 observations). To focus on the onset of high-level sick leave (HLSL; >16 days a year), we excluded individuals with HLSL during the survey year (baseline). We then used mixed-effect logistic regression models to assess prospective associations between self-reported work conditions and the occurrence of doctor-certified HLSL in the following year.

Results

The average occurrence of HLSL was 13.1%. After adjusting for sex, age, level of education, chronic health problems, and smoking, we observed an exposure-response relationship between cumulative exposure to work factors within all domains and the occurrence of HLSL. When evaluating the impact of combined exposures, predicted odds ratios (OR) for employees exposed to 1, 2, and 3 or more work factors within all domains were 1.60 (95%CI 1.32 − 1.94), 2.56 (95%CI 1.73 − 3.74) and 4.09 (95%CI 2.28 − 7.25), compared to those not exposed.

Conclusions

The results support the notion that exposure to multiple work factors in various domains, including psychosocial, mechanical, chemical, and physical work conditions, is associated with an increased risk of high-level sick leave. Employers and occupational health professionals should consider the joint impact of these domains when designing interventions.