bluuebunny/crossref_metadata_2025 huggingface dataset:
Antiseptic effectiveness of selected hydroalcoholic solutions used in medical laboratories in Benin
Authors: Dougnon Victorien TamÃ¨gnon , Bankole HonorÃ© Sourou , Dougnon Jacques Tossou , Koudokpon Hornel , Hounmanou Gildas , Monteiro Irina , Boko Michel , Lamine Baba-Moussa Lamine
Published: August 2021

Hand hygiene is identified as one of the best prevention methods especially at laboratory level. Thus, several hydroalcoholic solutions have been designed and marketed. These solutions are widely sold in the Republic of Benin and do not have the same chemical composition. The present study aimed to assess the bacteriological effectiveness of these solutions because of their odd multitude. 11 hydroalcoholic solutions were collected from phar-macies and labelled. The sensitivity of reference bacterial strains such as Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 was tested to these solutions. Thereafter, their ability to reduce bacterial colo-nies after impregnation of technicians’ hands was evaluated. At the end of the study, solutions A1, A8 and A10 were found active against S. aureus and E. coli. The inhibition diameters obtained were 13.5 and 29 mm respectively for A1, 20.5 and 32.5mm for A8 and 31 and 44.5 mm for solution A10. The rest were revealed inactive on the bacteria strains. After application on technicians’ hands, solutions A1, A8 and A10 showed reduction proportions of 60%, 4.30% and 41.67%, respectively. Apart from the ethanol contained in these three solutions, the presence of chlorhexidine enhances the effective-ness of the solution A10. These findings demonstrate that not all hand wash-ing hydroalcoholic solutions commercially available are effective. It is therefore important that concerned authorities evaluate the antibacterial perfor-mance of these products before being placed on the market in other to curb infections and protect populations’ health safety.
Association study of functional polymorphisms of DNMT3A and DNMT3B genes with breast carcinoma in north Indian population
Authors: Singh Shruti , Singh Kiran , Sachan Manisha
Published: August 2021

DNMT3A and DNMT3B are de novo methyltransferases which are responsi-ble for de novo methylation patterns of the unmethylated DNA. Two Single nucleotide polymorphisms (SNPs) in these genes i.e. -448A>G in DNMT3A and C46359T in DNMT3B, contribute a lot to the genetic susceptibility to breast cancer. In the present study, we analyzed the genotype frequencies of -448A>G polymorphism of DNMT3A and C46359T polymorphism of DNMT3B in breast cancer patients and healthy control subjects to explore the associa-tion of these single nucleotide polymorphisms with susceptibility to develop breast carcinoma. Genotyping was done by PCR-RFLP. 74 patients and 76 controls were genotyped for the DNMT3A (-448A>G) SNP, whereas 72 pa-tients and 107 controls were screened for DNMT3B (C46359T) polymor-phism. Our study clearly suggest that compared to GG carriers, the DNMT3A-448AA homozygotes had a 2.92 fold risk of developing breast carcinoma whereas for DNMT3B (C46359T) polymorphism, CT & CT+CC genotype carri-ers showed a 1.32 & 1.23 fold risk of developing breast carcinoma respec-tively. In Conclusion, DNMT3A SNP -448A>G contributes to genetic suscepti-bility to breast carcinoma whereas DNMT3B SNP C46359T was not found to be associated with pathogenesis of breast cancer in north Indian population.
Role of serotonin and noradrenalin in mechanisms of hypothalamic reg-ulation of experimental dystrophy of the retina
Authors: M. Miryusifova Ch. , I. Mohammadova S. , A. Azizov A. , G. Mammadov Z.
Published: August 2021

The features of the influence of electrical stimulation of the monoaminergic (MA) nuclei of the brain (nR, LC) on effects of the hypothalamic regulation of retinal electrogenesis in condition of experimental retinal dystrophy were studied It is shown that electrical stimulation of the suprachiasmatic nucleus of the hypothalamus (SCH) for 10 days on the background of the destruction of the pigment epithelium leads to the restoration of parameters as electro-retinogramm (ERG) and of the capacities of various structures of the visual analyzer. However, these effects are of short duration and quickly leveled. Preview activation of the various components MA-ergic system of the brain tends to have mixed influence of the effects of hypothalamic regulation. It is shown that the stimulation of nR increases the effects of SCH nuclei of the hypothalamus. In this environment, the recovery of the amplitude of the parameters from recommendation to photostimulation are stored during the time of testing (12 days). In contrast, stimulation of the LC leads to the oppo-site effects. In the current study, we discuss possible mechanisms of MA-ergic neuromodulation of plasticity of the various components of the visual system of the brain.
Etiology and Toxic Factors Involved in Male Infertility in Western Algeria
Authors: Anissa Fizazi , Malika Bendahmane , Zohraa Elhaina Fatima
Published: August 2021

The aims of this study were to determine the main etiology of male infertility and to assess the toxic factors responsible for this condition. We conducted a cross section study in a period of one year, starting from January 1st, 2014 to December 31st, 2014, on 320 patients consulting for fertility disorder at the Medical Assisted Procreation Unit (MAP) of Oran, in Western Algeria. The patients were interviewed using questionnaire inquiring about their demographics, general health issues, lifestyles and infertility factors. The results that the average age of patients was 40.39± 7.59 years; more than 73% of them were 35 years of age or older. The infertility was of primary type in 82% of patients; the average duration of infertility was 5.20± 3.79 years. Regarding the different etiology of male infertility, 29% of the patients had an idiopathic cause; 24% were due to varicocele and 22% were linked to genital tract infection. The study showed that toxic factors and life style that may impair male fertility concerned mainly advanced age group of men over 35 years (73.42%), tobacco smokers (70%), overweight men (62%) or occupational exposure to high heat (35.5%). Our conclusion is to educate infertile men so as to raise their awareness to the high risk factors such as toxic factors and lifestyle to enhance the natural fertility. We believe that this will allow better assessment and management of male infertility. We conclude that because of high idiopathic infertility rate, a further study using more patients is necessary.
Process Optimization for Sequential Recovery of N, P and K from Human Urine
Authors: Rahman Md. Azizur , Chariar Vijayaraghavan M
Published: August 2021

Human urine is considered as a potential source for nutrient source. In this paper we have presented a sequential two step process development for recovery of all three essential nutrient namely, nitrogen, phosphorus and potassium from human urine. In the first step the ammoniacal nitrogen was recovered by stripping process at a pH range of 10 -12, airflow rate of 2.5, 5 and 7.5L/min and temperature of 40ËšC and 50ËšC followed by precipitation of phosphorus and potassium recovery in the form of struvite precipitation in step two. The maximum ammonia recovered was 99.97% with the stripping condition of pH 12, temperature 50ËšC and air flow rate of 5.0L/min. The recovery phosphate and potassium ranges from 76-99.98% and 58-85.59% respectively. The precipitates characteristics were further analysed by EDX, SEM XRD and FTIR examination.
Vermibiotechnology: Relevance, Challenges and Future Prospects for India
Authors: Meghvansi Mukesh K , Khan Mohammad Haneef , Gupta Rajeev , Chaudhary K. K. , Siddiqui Sazada , Veer Vijay
Published: August 2021

In India and other developing countries, management of the urban solid waste is one of the most neglected areas of urban development. In addition, the continuous and indiscriminate use of easily soluble chemical fertilizers in high doses in agriculture has been found to be one of the major factors con-tributing to soil deterioration. Incidentally, earthworms have remarkable capability of effectively degrading waste biomass into vermicompost as their excreta. A major proportion of municipal solid waste generated is organic, which can be used as substrate for vermicomposting thereby minimizing pressure on the landfill sites. As the vermicompost application can significantly improve the soil fertility and in-turn plant health, the potential of earthworms in agriculture was realized soon and they became farmers’ friend. The modern researchers identified the environmental conditions optimal for earthworm activity for their effective utilization in organic waste degradation. Despite the worthwhile significance of vermibiotechnology in mu-nicipal solid waste management and in sustainable agriculture, it has not yet reached effectively to the stakeholders. This review attempts to elaborate the relevance of vermibiotechnology in Indian context for solid waste man-agement and in agriculture. Further, various challenges with respect to using this technology are highlighted. After detailed and critical analysis of the existing scientific literature, we also discuss future prospects of vermibi-otechnology in Indian context.
Studies on Bacillus SVTH6 Isolated from Leachate of Banaskantha District in Gujarat, India
Authors: Varjani Sunita J.
Published: August 2021

Industrial development in context with metal mining industries has received increasing importance now-a-days due to increased metal demand. With the concern of environment mining activities should be ecofriendly and economical. Bioleaching a biohydrometallurgical dissolution process, in which bacteria are used to leach out metals from ore. In bioleaching indigenous microbes either acidophilic, autotrophic, heterotrophic, or mixotrophic play important role. Bacteria of genera Acidithiobacillus, Leptospirillum, Sulfolobus, Sulfobacillus, Delftia sp., Oxalobacter sp., Bacillus sp. and Ferribacter sp. have been isolated from mining environment. In the study presented here thiosulphate utilizing Bacillus SVTH6 is used which was isolated from leachate sample of mining site. The organism was studied for thiosulphate utilization; cultural, morphological, cellular and biochemical characteristics; sugar utilization profile as well as antibiotic susceptibility. Growth optimization study of Bacillus SVTH6 at static and shaking condition as well as different pH and NaCl concentration(s) will be disussed in detail.
Ionic Liquids as Green Solvents for the Treatment of Endocrine Disrupting Compounds Using Liquid Membranes
Authors: Pilli Santhi Raju , Banerjee Tamal , Mohanty Kaustubha
Published: August 2021

Presence of endocrine disruptors in water and wastewater pose a serious threat to all living organisms. The removal of such disruptors is a major challenge especially most of the time they are present in trace amounts. Several technologies were tested to see if 100% removal can be achieved. Most of the existing technologies failed to achieve the target and have their own limitations. Membrane technology and especially liquid membrane technology has of late generated extreme interest among the researchers working with pollutants in trace amounts. In this work, experiments on three endocrine disruptors such as BPA, PCP and ES are carried out using supported ionic liquid membranes to see their removal efficiencies. The effects of various process parameters were studied to optimize them.
Isolation and Characterization of Cellulose Degrading Bacteria of Termite Gut from North Eastern Region of India
Authors: Sharma Sonika , Chatterjee Soumya , Datta Sibnarayan , Prasad Rajesh Kumar , Sharma Angkita , Dubey Dharmendra , Vairale Mohan G , Veer Vijay
Published: August 2021

A study was conducted to screen and isolate cellulase producing bacteria of termite gut from North east region of India. A total of 27 culturable bacterial isolates were screened for cellulase production. Out of the 27 bacterial isolates 11 showed zone of clearance on CMC agar media on staining with 1% Congo red, suggesting potential cellulose degrading activity. The maximum hydrolysis capacities (HC value) on CMC agar plate was found within the range of 3.6 to 40mm. The morphological characterization and gram staining of the positive isolates indicated that 3 isolates were positively stained rods and others were negative cocci. All the cellulase positive isolates were also tested for carbohydrate utilization with maltose, dextrose and fructose, to which all the 11 isolates responded positively. Further, based on the amplification and sequencing of the 16S rRNA genetic region, isolates were identified as member of the genus Bacillus, Paenibacillus and Staphylococcus. The degrading potential of these bacteria were assessed by developing bacterial consortium and efficient degradation was reported after seven days of incubation with different cellulose source like rice, cotton and rice husk.
A case study on the development of Swachh Gram by Vattavada Tribal Community: Way to Sanitation for All
Authors: Kalimuthu Arumugam , Ramesh S. Ramesh
Published: August 2021

Achieving Clean India by 2016 is the aim of the present government. While 50 % of Indians continue to defecate in openKerala State had ensured 96 % of sanitation coverage, with only 19 gram panchayats remaining as a challenge to declare the state as Nirmal Rajya State. Vattavada, in Devikulam block of Idukki District is one among those Panchayats, where hardly only 15% of the households had access to sanitation. A special project was conceived by the Suchitwa Mission of Kerala State Government which was ably supported by the local panchayat and Water, Sanitation and Hygiene Institute based at Kodaikanal to make the panchyat Open defecation. The advisory, implementation and funding support was given Government of India and Suchitwa Mission, Government of Kerala. The Technical support for toilet construction, demand creation and other need based IEC activities were carried out by WASH Institute. The district administration provided staff and monitoring support. The Suchitwa Mission also played a significant role in guiding, coordinating, supervising and helping in timely release of subsidy for the beneficiaries. The identification and selection of beneficiaries, execution and management of the project was done by Panchayat administration. The coordinated efforts of all these agencies resulted in 98 % sanitation coverage in the Panchayat and this case study captures the same.
Distribution levels of coherence relations of EEG nucleus rapher and locus coeruleus with the strucures of visual system of brain
Authors: Mamedov M. , Ganiyeva F. I.
Published: August 2021

Studies pertaining to the mechanisms and patterns of participation of different neuromodulatory centres of MA-ergic nature (n.raphe, locus coeruleus) in the regulation of neural processes in recent years are of particular importance when addressing the issues of optimization of neural processes both in normal and with different kinds of functional disorders. However, some aspects of the problem are not well understood. In particular, it is not the features of participating neuromodulatory centres in the organization of the electrical activity of different functional associations of the brain. The present work is devoted to analyse the distribution of coherent relations of the EEG neuromodulatory centres with the central structures of the visual system of brain. The results of the experiments indicated that 5-HT-ergic system characterized by a more significant correlation with the structures of visual analyzer than for NA-alicesme system of the brain. Thus the highest values of coherence potentials of nuclear rapher correspond to the theta and alpha ranges of the EEG.
Antimicrobial activity of phenolic extracts and essential oil from Thymelaea hirsuta
Authors: Deramchia Nawel , Belhakem Mostefa
Published: August 2021

In the current study, the antimicrobial activity of aqueous extract,organic solvents (petroleum ether, dichloromethane, methanol and ethanol extracts) as well as the essential oil of Thymelaea hirsuta was evaluated on various strains of dermal bacteria and fungi. Screening of phytochemicals was also carried out in order to determine the secondary metabolites available in the mixture (leaf / flower) of the plant. The activities of the T. hirsuta extracts were determined by diffusion method in agar medium for the bacteria and the direct contact procedure for the fungi for.The results obtained showed that all the extracts had an inhibitory activity on the growth of bacteria S. aureus and P. aeruginosa. The essential oil obtained by hydrodistillation showed the greatest influence on these strains. In addition, the antifungal activities of these extracts caused a short duration of the fungus activity (Microsporum audouinii, Microsporum gypseum and Trichophyton rubrum) and this effect depended on the concentrations of the extracts applied. The level of inhibition was also found to vary with procedure of the extraction used. Overall, the studied extracts have the potential to be used as antimi-crobial agents against the tested microbes.
Gastro-protective effects of camel milk on indomethacin-induced peptic ulcer in Wistar rats
Authors: Belhocine Mansouria , Homrani Abdelkader , Azzouz Fatima Azzouz , Sakmeche Chahrazed
Published: August 2021

Gastric ulcer associated with the use of nonsteroidal anti-inflammatory drugs (NSAIDs) and low-dose aspirin is a major public health problem. The present study was undertaken to determine the camel milk antioxidant activity, tested on DPPH, and its gastro-protective effect, investigated in Wistar rats sub-jected to gastric ulcer induced by a nonsteroidal anti-inflammatory agent, indomethacin. The study was performed on 20 adult male Wistar rats divided into 4 groups of 5 rats each. The negative control group received distilled water, the positive control group received indomethacin, the standard group received ranitidine and the fourth group was pretreated with raw camel milk, for 15 days respectively. On the 16th day, the indomethacin was ad-ministered to all rats except those of the negative control group. The ulcerogenic effect of indomethacin was highly significant, evidenced by a large number of ulcer lesions, a remarkably high ulcer index, and an important decrease in adherent gastric mucus. Camel milk resulted in significant gastro-protection compared to indomethacin ulcerated rats as manifested by significant decrease in ulcerative lesions number, and the ulcer index with a restored gastric mucus wall. The camel milk protection percentage is close to that of ranitidine. Additionally, in indomethacin-injured rats an increase in white blood cells, granulocytes, serum transaminases, and hemoglobin levels with a lowering in red blood cells were reported. These physiological disturbances were recovered by camel milk. Camel milk seemed to have gastro-protective effect, probably through its strong antioxidant activity, and may be recommended to patients with arthritis.
Study on relations among increase in blood pressure values, dispersions of the myocardial indicator , body mass index and thickness of the skin-fat layer in 15-17 year old schoolchildren in Baku, Azerbaijan
Authors: Mammadov A. M. , Aliyev A. Kh. , Panahova G. I. , Jafarova A. M.
Published: August 2021

In this study, relationship among arterial pressure (AP), systolic and diastolic pressure (SBP, DBP), heart beat frequency (HBF), dispersions of the myocardial indicator (MI), heart rate indicator (RI) and Body Mass Index (BMI) in schoolchildren of 15-17 years from Baku (Azerbaijan) were investigated. The results revealed that high values of above-mentioned indicators were gener-ally formed in groups with overweight and great distribution of the thickness of skin-fat layer (TSFL) on torso and top parts of the body. Aforementioned changes in these relationships indicate the formation of cardiometabolic syndrome in schoolchildren.
Physicochemical Analysis, Electrophoretic Characterization and Verifica-tion of the Protein Fractions Responsible for Celiac Disease of Wheat Varieties Imported and Grown in Western Algeria
Authors: Asma Remil , Mona Taghouti , Mohammed Benali , Slimane Belbraouet
Published: August 2021

Celiac disease is an inflammatory enteropathy induced by ingestion of wheat proteins. This study aims to verify the protein quality of wheat varieties that are either grown in or imported into Western Algeria, by carrying out physi-cochemical and electrophoretic characterisations of the gluten proteins. We carry out physicochemical analysis of thirty-four samples of durum and bread wheat, with regard to gluten proteins. The quality of gluten was ap-preciated by Zeleny volume of bread wheat and the SDS sedimentation test for durum wheat. Meanwhile, we also characterized glutenins and gliadins into thirteen cereal varieties by monodimensional electrophoresis on SDS-PAGE and Acid-PAGE respectively. The results showed that gluten levels ranges varied from 26.82±3.99% to 56.1±1.43%. The values of the total pro-tein content had the range of 11.35±0.42% to 20.57±0.98%. The varieties studied had Zeleny values between 7.79±1.45 mL to 40.07±2.96 mL for bread wheat, and the SDS sedimentation volume ranged from 24.5±0.70 mL to 51.25±2.47 mL for durum varieties. The analysis of the glutenin and gliadin composition of the wheat varieties by electrophoresis showed the existence of α- and ω-gliadins subunits, responsible for celiac disease. Furthermore, the results revealed the presence of gliadin subunits "γ-45" and glutenins "GS-LMW Type2" related to the good quality of gluten in some varieties of wheat. As a result, we can conclude that the wheat consumed in Western Algeria has a good quality of storage proteins. Therefore, it is far from being responsible for the decrease of celiac disease in this region.
Physicochemical Characterization and In-Vitro Antimicrobial Activity of Chitosan Extracted from Shrimp Shells Waste from Beni Saf Sea, Algeria
Authors: Youcefi Fatma , Riazi Ali , Mokhtar Meriem , Choukri Tefiani , Naima Khaouani
Published: August 2021

Chitosan is the most abundant natural organic polymer in nature. Its positive charge and its molecular arrangement confer interesting properties on the plane food, pharmaceutical, medical, cosmetic, water treatment. The present study was undertaken to study the physiochemical parameters and the in vitro antimicrobial activity of chitosan extracted from shrimp shells waste. The molecular weight of chitosan is 1414.33±16.99 kDa with, the percent of Ash 0,345±0,040 %, moisture is 2,98 ±0,13 % , and protein is 0.3 ±0,041 %.Chitosan produced (5 %) was also characterized with Fourier Transform Infrared Spectroscopy (FTIR) the spectrum of the chitosan sample from the shell recorded 16 peaks in the range of 689.40/cm and 3430.02 /cm. The antibacterial and antifungul activities of chitosan were examined against Escherichia coli ATCC10536, Pseudomonas aeruginosa ATCC 27853, Listeria monocytogenes ATCC7644, Staphylococcus aureus ATCC29213, Aspergillus niger ATCC 16888 and Candida albicans ATCC 10231 by agar wells diffusion the tests inhibitions zones diameters were 49,74± 0,75 , 54,35±0,93 , 42,27±1,07 , 32,95±0,28 and 53,73±0,64mm respectively. The minimum inhibitory concentration (MIC) using a broth microdilution method against tested microorganisms was ranging from 008% to 1.22%. These results open interesting perspectives of the chitosan. It can be used as new biomaterial with utility in many industrial areas.
Copper nanoparticles together with cold atmospheric plasma improves the growth and physiological indices of Dracocephalum moldavica in hydroponic system
Authors: Haddadian Kazhal , Iranbakhsh Alireza , Khavari-Nejad Ramazan Ali , Ghoranneviss Mahmood
Published: August 2021

This experiment was conducted to investigate the effect of copper nanoparticles and cold atmospheric plasma on growth and physiological indices of Dracocephalum moldavica herbal plant under hydroponic culture conditions. The factors investigated in this experiment were copper nanoparticles at four levels of zero (control), 25, 50, and 75 ppm and cold atmospheric plas-ma at three times of zero (control), 20, and 30 seconds. The results showed that application of cold atmospheric plasma led to an increase in the shoot height, root length, shoot dry weight, root dry weight, leaf area, chlorophyll a, chlorophyll b, total chlorophyll, soluble leaf protein; it also decreased proline content. In addition, there was no significant difference between cold atmospheric plasma at the two durations of 20 and 30 seconds. Moreover, compared with the control and non-nanoparticle copper treatments, the use of copper nanoparticles improved the measured indices; accordingly, when making its greatest effect, the use of copper nanoparticles led to 27.77% increase in shoot height, 73.76% in root length, 67.98% in shoot dry weight, 87.67% in root dry weight, 22.83% in leaf area, 76.19% in soluble leaf protein in treatment with 25 ppm of copper and 39.42%, 50%, and 42.36% increase in chlorophyll a, chlorophyll b, total chlorophyll, respectively in treatment with 50 ppm of copper nanoparticles. Considering the obtained results, it can be concluded that the use of 25 ppm of copper nanoparticles (due to the application of nanoparticle instead of non-nanoparticle copper) within 20 seconds of cold atmospheric plasma improves the growth and physiological indices of D. moldavica herbal plant under hydroponic culture conditions.
The effect of salinity on the growth, water status, and ion content on Common bean (Phaseolus vulgaris. L. var. El-Djadida)
Authors: Rahma Laredj-Zazou , Fawzia Toumi Benali , Sofiane Bouazza
Published: August 2021

Stress salinity has an important effect on crops physiology. The scope of our study was to evaluate the effect of salt stress tolerance as determined through growth attributes, water status and ion content in (Phaseolus vul-garis. L), the variety of El-Djadida in 6 weeks post stress application. The ex-periment was performed under glasshouse, in controlled conditions, in pots and irrigated with nutrient solution of Hoagland. Plants were irrigated with water containing sodium chloride alone (100 and 200 meq.l-1) combined with sodium chloride (NaCl) and calcium chloride (CaCl2) (100 and 150 meq.l-1). The results obtained showed that the salt application had a depressive effect on the organic growth however, this trend was dependant on the intensity of the stress. The hydric state of the plant varied with the concentration of sub-strate, thus exhibiting the ability to moderate the sensitive plant to adjust gradually to their osmotic pressure even by maintaining high concentrations of K+. The distribution of Na+, K+ and Ca++ in plant organs leaves and roots highlighted that the high level of salinity increased with levels of Na+ which inhibited the absorption of Ca++ and K+ ions.
A brief identification of lactic acid bacteria isolated from Algerian goatâ€™s Jben by MALDI-TOF MS
Authors: Meghoufel NaÃ¯ma L. , Homrani Abdelkader , Nemmiche SaÃ¯d , Benkrizi Nawel , Dahou Abdelkader E. , Zbalah Romaissa
Published: August 2021

In this paper, we investigated the lactic acid bacteria’s community of the Algerian goat’s Jben in order to define and preserve it. This cheese variety is only handmade with raw milk, and a dried flower of Cynara cardunculus is used instead of the animal rennet, it is also restricted in sub-Saharan prov-inces in Algeria, and no previous studies on its lactic acid bacteria composi-tion have been performed before. Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) procedure was used to identify 36 lactic acid bacteria isolated from artisanal Jben made from Arabia goat’s raw milk in Naâma (Algeria). The results showed the domination of the Enterococcus genus presents with four species: E. durans, E. faecalis, E. hirae and E. faecium. Lactococcus lactis and Leuconostoc mesenteroïdes were also detected. The species identified were favoured by the composition of goat’s raw milk microflora used and the artisanal manufacturing process of the cheese. The use of MALDI-TOF MS method provided us with a rapid diagnosis of the Jben lactic acid bacteria’s community. This method based on microbial abundant proteins fingerprint diagnosis was chosen for its fast, precise, low cost and less complicated analyse.
MALDI-TOF analysis of seasonal dynamics of Lactic Acid Bacteria from Algerian Goat Milk suggests predominance of Enterococcus Genus
Authors: Benkrizi Nawal , Homrani Abdelkader , Bekada Ahmed , Meghoufel Naima , Benbouziane Djilali , Adli Fatima Zohra
Published: August 2021

The goat milk is considered as nutraceutical due to its several beneficial com-pounds that increase its value. The purpose of this study was to investigate the variation of the goat milk’s lactic profile during winter and spring (2015). Several tests were realised. First, the isolation of the lactic acid bacteria con-tained in goat milk was realised by three specific media: the MRS, the M17, and the MSE. Second, a screening by the study of the proteolysis at two different concentration of casein (2%, 5%) and the study of aroma production were made to highlight performing isolates. These performing isolates were identified by using a precision spectrometry method which is called the Matrix Assisted Laser Desorption Ionisation Time-of-flight (MALDI-TOF). The identification with mass spectrometry «MALDI-TOF» technique revealed that those seasons had a more quantitative effect than a qualitative one. As a result, an average of 48% of lactic acid bacteria was identified for both sea-sons with a decrease of 6% observed in winter. In addition, it was revealed that Enterococcus was the most dominant genus in the Algerian goat milk. Therefore, lactic strains of this milk were proved to be technologically com-petent in terms of proteolysis and aroma production.
Prospective Assessment of Helicobacter Pylori Infection and Gastric Pathologies in Sidi Bel Abbes region, Western Algeria
Authors: Chama Zouaouia , Kanoun Khedoudj , Elkadi Fatima Zohra , Douidi Kara Turqui , Harir Noria , Kerkoud Mohamed , Abbouni Bouziyane
Published: August 2021

Helicobacter pylori infection concerns half of the world’s population, mainly in developing countries. It causes several gastrodudenal pathologies such as gastritis, ulcer and gastric adenocarcinoma. The aim of our study was to determine the prevalence of H.pylori infection and to assess the impact of different epidemiological factors as well as principal gastric diseases associ-ated to this infection. We underwent a prospective study during 18 months (month 2016-month 2017) which implicated 201 symptomatic patients for gastric fiboptic endoscopy at the level of Sidi Bel Abbes University hospital. We collected patients’ biopsies to perform a histological study and H. pylori culture. H. pylori identification was carried out based on bacteriological and biochemical analysis. The middle age of our population was (47.29 ±15.97ans) and the sex-ratio =0,8. The global prevalence of Helicobacter pylori infection is of 61.2% (123/201). This rate, after a statistic analysis, seems to be significantly related to age. It is particularly high especially for patients belonging to age range (20-30)-(51-60) years. The gender did not affect the infection prevalence that is more frequent in the gastritis case. We noticed also that HP infection prevalence was important in SBA the hospital. The range age (20-30)-(51-60) years had the highest prevalence of H. pylori and of gastritis which might be a risky ground of gastric cancer appearance. The ulcer pathology maximal rate concerned the group of 51 to 60 years. Above this age, this rate dropped whereas the number of patients suffering from gastric cancer, which presents an important rate in our study, increase for the group of 61-70 years.
Ephemeral Growth span and Rapid Veg-repo shift is Critical for Survival against Naturally Rainfed Upland and Erratic Drought in Rice Growing Regions
Authors: Kumar Prafull , Netam R S , Thakur A K , Kumari Poonam
Published: August 2021

Two experiments were undertaken simultaneously (early generation evalua-tion and advanced generation evaluation respectively) during Kharif 2013-14 at S G College of Agriculture and Research Station, Jagdalpur, IGKV, Raipur, Chhattisgarh, to indentify and analyze ephemeral genotypes for their suitability in rainfed upland and present era’s climate change-laid unpredicted water stress production agro-ecosystem. In early generation testing, mean plot flowering was recorded to be 72 Days After Sowing (DAS), plant height 83cm, panicles per square meter 212 and grain yield to be 2330kg/ha. Regional check Vandana and CRR-676-1 flowered earliest by 68 DAS followed by CRR-627-35-1-5 (69 DAS) and CRR-507-11-B-1 and CRR-605-23-1. The CRR-433-2-1-1 flowered latest by 83 DAS and as per hypothesis; yield was con-siderably reduced since plant could not develop optimum source-sink bal-ance and carbon accumulation in seeds eventually. Regarding, crop yield, five genotypes placed above the local check, namely CRR-597-5-1 (29.47%), the national check i.e. Anjali (21.05%), CRR-676-1 (15.79%), CRR-433-2-1-1 (10.53%) and the regional check i.e. Vandana (7.92%). Among advanced ma-terial, interestingly, the highest yielding genotype flowered earliest (69 DAS) that suggests the accomplishment of crop growth and physio-biochemical development while utilizing the soil and environmental reserve appropriate-ly. On overall, mean yield was 2349kg/ha, whereas genotype CRR-616-B-2-54-1 ranked 1st with 2718 kg/ha yield, 69 days of flowering duration and short bold grains. The bimodal experiment concludes that ephemeral growth span and rapid veg-repo shift is critical for survival against naturally rainfed-upland and erratic drought in rice growing regions and those genotypes which maintain the physiological buffer at the shift will be able to sustain genetic yield potential.
Impact of milking modalities on the occurrence of subclinical mastitis and the anti-bacterial effect of honey on isolated bacteria (Escherichia coli)
Authors: Nadia Zidane , Kheira Ghazi
Published: August 2021

Prevention of mastitis is a major concern in dairy cattle breeding, and is among the most costly diseases of dairy companies. The purpose of this study was to compare two types of milking practices; one using manual milk-ing and the other using mechanical milking to determine milking type consequences on the occurrence and spread of subclinical mastitis. One hundred dairy cows were brought to the slaughterhouse following a fall in milk production due to subclinical mastitis, of which 64 cows were mechanically milked and 36 cows were manually milked. After anti-mortem and post-mortem examinations, we found that 58% of cows in mechanical milking mode had mastitis. Amongst them 9% of cows had mastitis spread on the four teats. However, the rate was higher (68%) in cows where milking was done manually, of which 49% mastitis spread on the four teats. The bacterio-logical analyses was carried out regarding coliforms, Staphylococci and Streptococci on the raw milks of cows suspected of mastitis, irrespective of the mode of milking.. We chose E. coli and found that its resistance against antibiotics tested was important, except Gentamicin where the sensitivity was average with an inhibition zone of 1.6 cm. We then evaluated the effect of pure honey on the strain to see its antiseptic effect. We noticed the inhibition zone diameters of 2.2 cm. We also tested the effect of diluted honey, the results of which were not satisfactory because of the decreased antisep-tic activity possibly owing to decreased acidity of the honey.
Investigation of coherent relationships of EEG in the structures of the visual analyzer of rabbits before and after experimental epilepsy, creat-ed by the sodium salt of penicillin
Authors: H. Aliyev A. , M. Miryusifova Ð¡h. , A. Aliyeva F. , Sh. Ibrahimova S.
Published: August 2021

The paper considers effects of the sodium salt of penicillin, on the bioelectric activity of the visual analyzer structures in rabbits. Penicillin was injected into the amygdale, and a focus was created in this structure. Modeling the epileptic focus in amygdale allows to detect the spread of excitation as in motor structures of the brain (clonic convulsions), and in various structures of the visual analyzer. A comparative analysis of the electrical activity of spe-cific (visual cortex, superior colliculus) and non-specific structures (suprachiasmatic nucleus of the hypothalamus) of the visual analyzer was performed in norm and after epileptic attacks. For this purpose, a coherent analysis of the electrical activity of the above-mentioned structures was per-formed in normal and after epileptic seizures. The experiments were carried out on rabbits. Registration and analysis of the electroencephalogram of various brain structures was performed by using the automated system "Brainsys". It is also established that there is a change in the coherent bonds between the structures of the visual analyzer after epileptic seizures. The coherence coefficient, except for the alpha range, increased in all structures compared with the background. The change in coherent bonds after epileptic seizures is explained by the activation of functional interactions between the structures of the central nervous system. The presence of high-amplitude theta and delta waves in the electroencephalogram indicates functional changes in the central nervous system. It is known that the visual analyzer has complex differentiated properties of plasticity. After epilepsy with the help of endogenous compensatory mechanisms it forms a new rela-tionship between the structures of the central nervous system.
Optimization of Manufacturing Process of Traditional Algerian Jben Cheese Using Response Surface Methodology (RSM)
Authors: Amimour M. , Idoui T. , Cheriguene A.
Published: August 2021

The Aim of this study was to develop an optimized method for manufacturing process of traditional Algerian Jben cheese, using response surface methodology (RSM). In order to develop the objective method of making this traditional cheese, several factors have been studied and a Plackett-Burman statistical design was applied. The effects of the four screened factors (enrichment with milk powder, 10 - 20 g/l; pH of milk, 5.75 - 6.75, enzymatic extract dose, 0.5 - 1.5 ml and coagulation temperature 40 - 60 °C) on the response were investigated, using a Box-Behnken statistical design. Multiple regression analysis was used so that experimental data fits to a second-order polynomial equation. This multiple analysis showed that the model explains about 90.73% of the variation. Based on statistical results, it can be noticed that enrichment with milk powder and pH of milk (Ë‚0.0001***) were highly significant factor influincing cheese yield. The optimal production parame-ters that maximized cheese product (20 g/l enrichment with milk powder, 5.75 pH of milk, 1.29 ml enzymatic extract dose and 60°C coagulation temperature) and the maximal predicted cheese yield (52.68 % ) were found out through response surface methodology. Under these conditions, a verification experiment was carried out and cheese yield was found to be 49.46 %. The overall percentage of agreement for the experimental results (more than 93 % validity) with the predicted values indicates the validation of the statistical model and the success of the optimization process.
Physico-chemical, quality parameters and composition of Chemlal extra virgin olive-oils from Algeria
Authors: Iddir A. , Bekada A M. A. , Kiciri S. , Boualit S.
Published: August 2021

The aim of this work was to determine the composi on, physicochemical and quality parameters of Chemlal EVOO from di erent regions of Algeria and at di erent harvest me. Olive-oil yield, quality indices, fa y acid composition, pigments (carotenoids and chlorophylls), phenolic compounds were evaluated for a complete descrip on of olive-oil samples. The nal results showed that the altitude and the me of harvest obviously had an in uence on the quality and the chemical composition of the olive oils. A very advanced maturity was observed for the olives coming from the low altitudes. The olives of the region of Oran at 80 m of al tude ripen very quickly that the olives of M'chedallah to 474 m and more than those of the region of Illit- en which is more than 700 m. The pro le of fa y acids was in uenced by al tude. Oleic acid, which is a nutri onal and quality criterion for olive oil, increases with al tude but does not exceed the limit set by the Interna onal Olive Council. On the other hand, the content of pigments and phenolic compounds, decreased with the matura on of olives. According to the results found, the most suitable ripening index for harves ng olives for Chemlal EVOOs of high chemical quality starts from 3.20 up to 4.
Antibacterial activity of Lactobacilli detected in Algerian raw honeys against gram-negative bacteria
Authors: Mounia Homrani , Fatiha Dalache , Mohammed Bouzouina , Said Nemmiche , AbdelKader Homrani
Published: August 2021

This study reports the isola on of Lactobacilli from Four (n=04) typical Apis Mellifeca raw honey samples collected from different regions in Algeria and the evalua on of their an bacterial ac vi es against Gram-negative bacteria. The isolation of Lactobacilli was performed using different media. Isolates were identified  firrstly by catalase test, Gram staining and cells morphology, and confirmed by MALDI-TOF-MS. An bacterial activities of Bacterial cultures of selected Lactobacilli and their cell free supernatants were tested by agar spot assays and agar well diffusion assay. A total of eighteen (n=18) bacteria isolated from honey samples were presump vely iden ed as Lac- tobacillus spp. based on their posi ve Gram reactions, absence of catalase and their rod shape. MALDI-TOF MS iden ca on confirmed that all isolates were classified as Lactobacillus genus, but the results for their species were inconclusive with uncertainty between, Lactobacillus pentosus, Lactobacillus plantarum and Lactobacillus paraplantarum. Isolates exhibited an microbial activity with inhibition zone diameters ranging from 11 ± 1.41 mm to 17.5 ± 0.71 mm. 6 on 11 Lactobacilli Supernatants demonstrated inhibitory ac vity against all target bacteria. This study reveals the existence of Lactobacilli in Algerian raw Honeys. These Lactobacilli possess an bacterial properties against Gram-negative bacteria, often responsible of human infections, and can be a favorable substitute to antibiotics.
Phytochemical Screening And Evaluation Of The Antioxidant Activity Of Essential Oil Of Pistacia Atlantica Harvested From The Ouled Brahim Region, Saida; Algeria
Authors: Fatima Attou , Mohamed Terras , Abdelkader Ammam , Faiza Tabet Aouel
Published: August 2021

The knowledge of traditional healers o en represents a basis for the pharmacological and phytochemical inves ga on of naturals medicaments. This work aims for the phytochemical study and evaluation of the antioxidant activity of essential oils of medicinal plant Pistacia atlantica.Through the results obtained, it appears that the leaves are rich in substances with a high antioxidant power. For essential oils, it is 0.24 % for 100 g of plant material. And the phytochemical screening of the plant showed the presence of tannins, resins, coumarins, terpenoids and  flavonoids, and absence of saponins. The strong antioxidant activity of extracts obtained only confirms the traditional use of this plant by the local population.
Phenotypic and Molecular Characterization of Lactobacilli Isolated from breast-fed Infants faeces in Bechar region,South West of Algeria
Authors: Ahmed Yagoubi , Ahmed Bensoltane
Published: August 2021

Lactobacilli are of great importance to humans, as they are essential members of a healthy human microbiota at various niches and serve as biologically and functionally important components of a healthy human diet. For this reason, the isolation of lactobacilli strains from infant feces (less than 6 months old) was carried out in Bechar region, Algeria. In total, 62 strains were isolated, purified, and identified based on the physiological and biochemical methods. For final confirmation, API 50 CHL gallery was used. The iden ca on and phylogenetic analysis of isolated strains was performed by sequencing the 16S rRNA gene, which led us to identify four species with different percentages; Lactobacillus rhamnosus, L. fermentum, L.mucosa, L. plantarum. With regard to the characteristics of technological interest, most of the identified strains exhibited good lactic acid production. However, it was not until after 3 hours of incubation at 37 °C that isolated lactobacilli produced a maximum acidity, which reached Δ pH 0.54 in the reconstituted milk. The results were different with camel milk, in the first time, and this was observed in the acidity rate which was generally lower in this type of milk in which acidity reached Δ pH 0.47 .As regards the proteolytic power, the strains showed a high level of proteolysis. Regarding the lipolytic activity, it was observed that none of the lactobacilli tested expressed lipolytic activity in the presence of buffer and olive oil, whereas the results of the strains were different as regards the flavoring activity and the texturing activity.
Production and quality assessment of a set-style yogurt fortified with low concentrations of Thymus vulgaris L phenolic extracts
Authors: Khelifi Haroune , Saada Djamel Ait , Bekada Ahmed Mohamed Ali , Dehimeche Nafissa
Published: August 2021

The aim of this work was to evaluate the effect of Thymus vulgaris L phenolic extracts (TVPE) on the growth of Streptococcus thermophilus and Lactobacil- lus delbrueckii subsp. bulgaricus, and the impact of their addition in small amounts (2-8%) to a set-style yogurt with regard to variations in physico- chemical, bacteria count and organoleptic properties during 21 days storage at 4°C. The phenolic components were extracted from the crushed aerial parts of the test plant by cold maceration in hydromethanolic solution (methanol: water, 80:20 v/v). The plant polyphenolic profile was determined by HPLC. The survival ability of yogurt starter cultures in the presence of TVPE was evaluated through the determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The total phenolic content (TPC) was 20.2 ± 0.57 μg GAE / mg extract. The amount of  flavonoid compounds was 39.83 μg EQ/mg extract. As the quantitative measurements revealed, apigenin and fisetin (8.17, 6.83, μg GAE /mg extract respectively) emerged as major phenolic compounds from thyme aerial parts. The MIC and MBC against the growth of both lactic bacteria were obtained at 60% of TVPE concentration. A significant (p< 0.01) changes in pH, trable acidity and apparent viscosity were observed in all experimental yogurts. The best scores of sensory evaluation were recorded in samples added with 2% and 4% of TVPE as well as the control. Based on collected data, 2% and 4% of TVPE can be incorporated into yogurt without risk of deterioration in physicochemical quality or inhibition of lactic bacteria.