bluuebunny/crossref_metadata_2025 huggingface dataset:
Radial Flow Perfusion Enables Real-Time Profiling of Cellular Metabolism at Low Oxygen Levels with Hyperpolarized 13C NMR Spectroscopy
Authors: Mancuso Anthony , Pourfathi Mehrdad , Kiefer Ryan M. , Noji Michael C. , Siddiqui Sarmad , Profka Enri , Weber Charles N. , Pantel Austin , Kadlecek Stephen J. , Rizi Rahim , Gade Terence P. F.
Published: August 2021

In this study, we describe new methods for studying cancer cell metabolism with hyperpolarized 13C magnetic resonance spectroscopy (HP 13C MRS) that will enable quantitative studies at low oxygen concentrations. Cultured hepatocellular carcinoma cells were grown on the surfaces of non-porous microcarriers inside an NMR spectrometer. They were perfused radially from a central distributer in a modified NMR tube (bioreactor). The oxygen level of the perfusate was continuously monitored and controlled externally. Hyperpolarized substrates were injected continuously into the perfusate stream with a newly designed system that prevented oxygen and temperature perturbations in the bioreactor. Computational and experimental results demonstrated that cell mass oxygen profiles with radial flow were much more uniform than with conventional axial flow. Further, the metabolism of HP [1-13C]pyruvate was markedly different between the two flow configurations, demonstrating the importance of avoiding large oxygen gradients in cell perfusion experiments.
Towards Application-Driven Task Offloading in Edge Computing Based on Deep Reinforcement Learning
Authors: Sun Ming , Bao Tie , Xie Dan , Lv Hengyi , Si Guoliang
Published: August 2021

Edge computing is a new paradigm, which provides storage, computing, and network resources between the traditional cloud data center and terminal devices. In this paper, we concentrate on the application-driven task offloading problem in edge computing by considering the strong dependencies of sub-tasks for multiple users. Our objective is to joint optimize the total delay and energy generated by applications, while guaranteeing the quality of services of users. First, we formulate the problem for the application-driven tasks in edge computing by jointly considering the delays and the energy consumption. Based on that, we propose a novel Application-driven Task Offloading Strategy (ATOS) based on deep reinforcement learning by adding a preliminary sorting mechanism to realize the joint optimization. Specifically, we analyze the characteristics of application-driven tasks and propose a heuristic algorithm by introducing a new factor to determine the processing order of parallelism sub-tasks. Finally, extensive experiments validate the effectiveness and reliability of the proposed algorithm. To be specific, compared with the baseline strategies, the total cost reduction by ATOS can be up to 64.5% on average.
Bidirectional Active Piezoelectric Actuator Based on Optimized Bridge-Type Amplifier
Authors: Huang Weiqing , Lian Junkai , Chen Mingyang , An Dawei
Published: August 2021

Piezoelectric actuators based on bridge displacement amplifying mechanisms are widely used in precision driving and positioning fields. The classical bridge mechanism relies on structural flexibility to realize the return stroke, which leads to the low positioning accuracy of the actuator. In this paper, a series bridge mechanism is proposed to realize a bidirectional active drive; the return stroke is driven by a piezoelectric stack rather than by the flexibility of the structure. By analyzing the parameter sensitivity of the bridge mechanism, the series actuation of the bridge mechanism is optimized and the static and dynamic solutions are carried out by using the finite element method. Compared with the hysteresis loop of the piezoelectric stack, the displacement curve of the proposed actuator is symmetric, and the maximum nonlinear error is improved. The experimental results show that the maximum driving stroke of the actuator is 129.41 μm, and the maximum nonlinear error is 5.48%.
Direct-on-Filter FTIR Spectroscopy to Estimate Calcite as A Proxy for Limestone ‘Rock Dust’ in Respirable Coal Mine Dust Samples
Authors: Pokhrel Nishan , Keles Cigdem , Jaramillo Lizeth , Agioutanti Eleftheria , Sarver Emily
Published: August 2021

Application of fine, inert ‘rock dust’ (RD) to the surfaces in underground coal mines is a common method for mitigating coal dust explosion hazards. However, due to its size, RD has the potential to contribute to the respirable coal mine dust (RCMD) concentration. Though the RD component of RCMD does not appear to pose the sort of health hazards associated with other components such as crystalline silica, understanding its relative abundance may be quite helpful for evaluating and controlling primary dust sources. Given that RD products are frequently comprised of high-purity limestone (i.e., primarily calcite mineral), calcite may serve as a suitable proxy for measuring RD. To estimate the mass percentage of calcite in RCMD samples, this study demonstrates the successful application of direct-on-filter (DOF) Fourier-transform infrared (FTIR) spectroscopy. Incidentally, DOF FTIR has been the focus of recent efforts to enable rapid measurement of crystalline silica in RCMD. Concurrent measurement of other constituents such as calcite is thus a logical next step, which can allow a broader interpretation of dust composition and source contributions.
Cinnamal Sensing and Luminescence Color Tuning in a Series of Rare-Earth Metal−Organic Frameworks with Trans-1,4-cyclohexanedicarboxylate
Authors: Demakov Pavel A. , Vasileva Alena A. , Volynkin Sergey S. , Ryadun Alexey A. , Samsonenko Denis G. , Fedin Vladimir P. , Dybtsev Danil N.
Published: August 2021

Three isostructural metal–organic frameworks ([Ln2(phen)2(NO3)2(chdc)2]·2DMF (Ln3+ = Y3+ for 1, Eu3+ for 2 or Tb3+ for 3; phen = 1,10-phenanthroline; H2chdc = trans-1,4-cyclohexanedicarboxylic acid) were synthesized and characterized. The compounds are based on a binuclear block {M2(phen)2(NO3)2(OOCR)4} assembled into a two-dime nsional square-grid network containing tetragonal channels with 26% total solvent-accessible volume. Yttrium (1)-, europium (2)- and terbium (3)-based structures emit in the blue, red and green regions, respectively, representing the basic colors of the standard RGB matrix. A doping of Eu3+ and/or Tb3+ centers into the Y3+-based phase led to mixed-metal compositions with tunable emission color and high quantum yields (QY) up to 84%. The bright luminescence of a suspension of microcrystalline 3 in DMF (QY = 78%) is effectively quenched by diluted cinnamaldehyde (cinnamal) solutions at millimolar concentrations, suggesting a convenient and analytically viable sensing method for this important chemical.
Mexican Oregano (Lippia graveolens Kunth) as Source of Bioactive Compounds: A Review
Authors: Bautista-Hernández Israel , Aguilar Cristóbal N. , Martínez-Ávila Guillermo C. G. , Torres-León Cristian , Ilina Anna , Flores-Gallegos Adriana C. , Kumar Verma Deepak , Chávez-González Mónica L.
Published: August 2021

Lippia graveolens is a traditional crop and a rich source of bioactive compounds with various properties (e.g., antioxidant, anti-inflammatory, antifungal, UV defense, anti-glycemic, and cytotoxicity) that is primarily cultivated for essential oil recovery. The isolated bioactive compounds could be useful as additives in the functional food, nutraceuticals, cosmetics, and pharmaceutical industries. Carvacrol, thymol, β-caryophyllene, and p-cymene are terpene compounds contained in oregano essential oil (OEO); flavonoids such as quercetin O-hexoside, pinocembrin, and galangin are flavonoids found in oregano extracts. Furthermore, thermoresistant compounds that remain in the plant matrix following a thermal process can be priced in terms of the circular economy. By using better and more selective extraction conditions, the bioactive compounds present in Mexican oregano can be studied as potential inhibitors of COVID-19. Also, research on extraction technologies should continue to ensure a higher quality of bioactive compounds while preventing an undesired chemical shift (e.g., hydrolysis). The oregano fractions can be used in the food, health, and agricultural industries.
Analysis of Processing Effects on Glucosinolate Profiles in Red Cabbage by LC-MS/MS in Multiple Reaction Monitoring Mode
Authors: Wu Weicheng , Chen Jingqiu , Yu Dandan , Chen Shiguo , Ye Xingqian , Zhang Zhiguo
Published: August 2021

Red cabbage (Brassica oleracea L. var. capitata) continues to receive increasing attention on its health-promoting properties because of its high glucosinolate content. Glucosinolates are an unstable active substance; however, there are few studies on their changes in different cooking processes. In this study, we investigated the effects of processing methods (boiling, steaming, microwave heating, frying, stir-frying) and boiling time on glucosinolates in red cabbage. Ten glucosinolates, including 4-methoxyglucobrassicin, neoglucobrassicin, glucoalyssin, glucobrassicin, glucoraphanin, glucoiberin, progoitrin, gluconapin and sinigrin, in red cabbage were detected. Decreases of 32.36%, 24.83%, 25.27%, 81.11% and 84.29% for total glucosinolates were observed after boiling, microwaving, steaming, frying and stir-frying. Indole glucosinolates were more efficiently lost compared to aliphatic glucosinolates after boiling, while microwaving, steaming, frying and stir-frying also resulted in a greater reduction in indole glucosinolates than aliphatic glucosinolates. Glucoalyssin, glucoerucin and sinigrin were more thermal sensitive than other glucosinolates. It was confirmed that microwaving and steaming retained higher levels of glucosinolates than other methods and may be better for cooking red cabbage.
Insight into the Way the Content of Biologically Active Compounds in Meadowsweet Inflorescences (Filipendula ulmaria (L.) Maxim.) Is Shaped by Phytosociological Habitats
Authors: Stawarczyk Kinga , Chrupek Aleksandra , Sękara Agnieszka , Gostkowski Michał , Karbarz Małgorzata
Published: August 2021

A collection of herbs from the natural environment remains not only a source of raw material but also provides evidence of chemical differentiation of the local populations. This work aimed at performing a phytosociological analysis of seven different stands of meadowsweet (Filipendula ulmaria (L.) Maxim.) occurrence. A determination of total phenolic compounds and salicylates and the antioxidant activity of dried meadowsweet inflorescences (Flos ulmariae) was also performed. Active chemical compounds in F. ulmaria inflorescences were related to chemotype and diversified between investigated populations. Geographical distance and variation in phytosociological locations affected chemical composition in different ways, shaping the content of biochemical compounds crucial for herbal material quality. The obtained results can be a valuable indicator for Nexo and Baligród populations, which are good genetic material for research, breeding, and cultivation due to their biochemical composition, especially with respect to salicylates, as major compounds of determining market quality of Flos ulmariae.
PET Imaging Radiotracers of Chemokine Receptors
Authors: Alluri Santosh R. , Higashi Yusuke , Kil Kun-Eek
Published: August 2021

Chemokines and chemokine receptors have been recognized as critical signal components that maintain the physiological functions of various cells, particularly the immune cells. The signals of chemokines/chemokine receptors guide various leukocytes to respond to inflammatory reactions and infectious agents. Many chemokine receptors play supportive roles in the differentiation, proliferation, angiogenesis, and metastasis of diverse tumor cells. In addition, the signaling functions of a few chemokine receptors are associated with cardiac, pulmonary, and brain disorders. Over the years, numerous promising molecules ranging from small molecules to short peptides and antibodies have been developed to study the role of chemokine receptors in healthy states and diseased states. These drug-like candidates are in turn exploited as radiolabeled probes for the imaging of chemokine receptors using noninvasive in vivo imaging, such as positron emission tomography (PET). Recent advances in the development of radiotracers for various chemokine receptors, particularly of CXCR4, CCR2, and CCR5, shed new light on chemokine-related cancer and cardiovascular research and the subsequent drug development. Here, we present the recent progress in PET radiotracer development for imaging of various chemokine receptors.
Numerical Study of Ultra-Broadband Metamaterial Perfect Absorber Based on Four-Corner Star Array
Authors: Cheng Yu , Xiong Min , Chen Ming , Deng Shijie , Liu Houquan , Teng Chuanxin , Yang Hongyan , Deng Hongchang , Yuan Libo
Published: August 2021

In recent years, research on solar absorbers provides a significant breakthrough to solve the energy crisis. A perfect solar absorber based on a four-corner star array is designed and the absorption performance is analyzed numerically. The results show that the absorber reaches more than 90% of the full band in the range of 400–2000 nm. In particular, the absorption efficiency of the continuous more than 95% of the bandwidth reached 1391 nm, and the average absorption efficiency of the whole study band is more than 98%, and the loss of the solar spectrum only accounted for 2.7%. At the same time, the absorption efficiency can be adjusted by changing the geometric structure of the absorber. In addition, due to the perfect symmetry of the structure, it has an excellent insensitivity of the incident angle and polarization angle. In general, the proposed solar absorber has exciting prospects in solar energy collection and utilization, photothermal conversion and other related fields.
Synthesis of Fe3O4@mZrO2-Re (Re = Y/La/Ce) by Using Uniform Design, Surface Response Methodology, and Orthogonal Design & Its Application for As3+ and As5+ Removal
Authors: Alam Easar , Feng Qiyan , Yang Hong , Fan Jiaxi , Mumtaz Sameena , Begum Farida
Published: August 2021

In this study, iron oxide (Fe3O4) was coated with ZrO2, and doped with three rare earth elements((Y/La/Ce), and a multi-staged rare earth doped zirconia adsorbent was prepared by using uniform design U14, Response Surface methodology, and orthogonal design, to remove As3+ and As5+ from the aqueous solution. Based on the results of TEM, EDS, XRD, FTIR, and N2-adsorption desorption test, the best molar ratio of Fe3O4:TMAOH:Zirconium butoxide:Y:La:Ce was selected as 1:12:11:1:0.02:0.08. The specific surface area and porosity was 263 m2/g, and 0.156 cm3/g, respectively. The isothermal curves and fitting equation parameters show that Langmuir model, and Redlich Peterson model fitted well. As per calculations of the Langmuir model, the highest adsorption capacities for As3+ and As5+ ions were recorded as 68.33 mg/g, 84.23 mg/g, respectively. The fitting curves and equations of the kinetic models favors the quasi second order kinetic model. Material regeneration was very effective, and even in the last cycle the regeneration capacities of both As3+ and As5+ were 75.15%, and 77.59%, respectively. Adsorption and regeneration results suggest that adsorbent has easy synthesis method, and reusable, so it can be used as a potential adsorbent for the removal of arsenic from aqueous solution.
Overview of Beneficial Effects of (Poly)phenol Metabolites in the Context of Neurodegenerative Diseases on Model Organisms
Authors: Carregosa Diogo , Mota Sara , Ferreira Sofia , Alves-Dias Beatriz , Loncarevic-Vasiljkovic Natasa , Crespo Carolina Lage , Menezes Regina , Teodoro Rita , Santos Cláudia Nunes dos
Published: August 2021

The rise of neurodegenerative diseases in an aging population is an increasing problem of health, social and economic consequences. Epidemiological and intervention studies have demonstrated that diets rich in (poly)phenols can have potent health benefits on cognitive decline and neurodegenerative diseases. Meanwhile, the role of gut microbiota is ever more evident in modulating the catabolism of (poly)phenols to dozens of low molecular weight (poly)phenol metabolites that have been identified in plasma and urine. These metabolites can reach circulation in higher concentrations than parent (poly)phenols and persist for longer periods of time. However, studies addressing their potential brain effects are still lacking. In this review, we will discuss different model organisms that have been used to study how low molecular weight (poly)phenol metabolites affect neuronal related mechanisms gathering critical insight on their potential to tackle the major hallmarks of neurodegeneration.
Maslinic Acid Attenuates Denervation-Induced Loss of Skeletal Muscle Mass and Strength
Authors: Yamauchi Yuki , Ferdousi Farhana , Fukumitsu Satoshi , Isoda Hiroko
Published: August 2021

Maslinic acid (MA) is a pentacyclic triterpene abundant in olive peels. MA reportedly increases skeletal muscle mass and strength in older adults; however, the underlying mechanism is unknown. This study aimed to investigate the effects of MA on denervated muscle atrophy and strength and to explore the underlying molecular mechanism. Mice were fed either a control diet or a 0.27% MA diet. One week after intervention, the sciatic nerves of both legs were cut to induce muscle atrophy. Mice were examined 14 days after denervation. MA prevented the denervation-induced reduction in gastrocnemius muscle mass and skeletal muscle strength. Microarray gene expression profiling in gastrocnemius muscle demonstrated several potential mechanisms for muscle maintenance. Gene set enrichment analysis (GSEA) revealed different enriched biological processes, such as myogenesis, PI3/AKT/mTOR signaling, TNFα signaling via NF-κB, and TGF-β signaling in MA-treated mice. In addition, qPCR data showed that MA induced Igf1 expression and suppressed the expressions of Atrogin-1, Murf1 and Tgfb. Altogether, our results suggest the potential of MA as a new therapeutic and preventive dietary ingredient for muscular atrophy and strength.
Healthy Breastfeeding Infants Consume Different Quantities of Milk Fat Globule Membrane Lipids
Authors: George Alexandra D. , Gay Melvin C. L. , Selvalatchmanan Jayashree , Torta Federico , Bendt Anne K. , Wenk Markus R. , Murray Kevin , Wlodek Mary E. , Geddes Donna T.
Published: August 2021

The human milk fat globule membrane (MFGM) contains important lipids for growing infants. Anthropometric measurements, milk samples, and infant milk intake were collected in a cohort of eleven healthy mother–infant dyads during exclusive breastfeeding from birth to six months. One hundred and sixty-six MFGM lipids were analysed using liquid chromatography-mass spectrometry, and the infant intake was calculated. The concentrations and intake were compared and associations between infant intake and growth characteristics explored. The lipid concentrations and infant intake varied widely between mother–infant dyads and between months one and three. The infant intake for many species displayed positive correlations with infant growth, particularly phospholipid species. The high variation in lipid intake is likely an important factor in infant growth, with strong correlations identified between the intake of many MFGM lipids and infant head circumference and weight. This study highlights the need for intake measurements and inclusion in cohort studies to elucidate the role of the human milk lipidome in infant growth and development.
Assessment of Sport Nutrition Knowledge, Dietary Practices, and Sources of Nutrition Information in NCAA Division III Collegiate Athletes
Authors: Klein Dylan J. , Eck Kaitlyn M. , Walker Alan J. , Pellegrino Joseph K. , Freidenreich Daniel J.
Published: August 2021

Nutrition knowledge is a critical component of meeting sport nutrition guidelines. The present study aimed to evaluate the sport nutrition knowledge of National Collegiate Athletic Association (NCAA) Division III (DIII) athletes using a validated questionnaire, and to assess the dietary practices and sources of nutrition information in this population. A total of 331 student-athletes (n = 149 males, n = 181 females, n = 1 no sex indicated) completed the questionnaire. The mean score for total sport nutrition knowledge was 6.49 ± 8.9 (range −49 to 49) with a mean percent (%) correct score of 36.9 ± 19.1%. Athletes who had a previous college-level nutrition course (n = 62) had significantly higher (p < 0.05) total sport nutrition, carbohydrate, and hydration knowledge compared to those who did not (n = 268). Individual sport athletes (n = 90) scored significantly higher (p < 0.05) on hydration and micronutrients knowledge than team sport athletes (n = 237), while females scored higher than males for hydration knowledge (p < 0.05). The majority of athletes reported sensible dietary habits, such as not frequently skipping meals and eating carbohydrate and protein foods peri-workout. Athletes also reported their primary sources of nutrition information, the top three sources being social media, coaches, and athletic trainers, despite most frequently rating registered dietitians/nutritionists as “extremely knowledgeable”. Despite low sport nutrition knowledge, NCAA DIII collegiate athletes practiced seemingly prudent dietary habits but lacked exposure to high-quality sources of nutrition information.
Iron Deficiency in Vegetarian and Omnivorous Individuals: Analysis of 1340 Individuals
Authors: Slywitch Eric , Savalli Carine , Duarte Antonio Cláudio Goulart , Escrivão Maria Arlete Meil Schimith
Published: August 2021

The objective of this study was to evaluate the serum levels of ferritin and the prevalence of iron deficiency in vegan and omnivorous individuals by taking into account the presence of elements that cause an elevation of ferritin levels, such as increased homeostatic model assessment of insulin resistance (HOMA-IR), body mass index (BMI), and high-sensitivity C-reactive protein (hs-CRP) values. The parameters were evaluated in 1340 individuals, i.e., 422 men and 225 women who do not menstruate and 693 women who do menstruate, based on omnivorous or vegetarian eating habits. The progressive increase in BMI, HOMA-IR, and inflammation caused an elevation in ferritin concentration, regardless of the eating habits in the groups studied. In the overall sample, omnivores had a higher prevalence of obesity, higher ferritin levels, and a lower prevalence of iron deficiency (ferritin < 30 ng/mL). However, after the exclusion of individuals with inflammation (with overweight/obesity and elevated hs-CRP levels), the actual iron deficiency was assessed and was not higher among vegetarians, except in women with regular menstrual cycles. Our data show that nutritional status and inflammation levels affect ferritin levels and may interfere with the correct diagnosis of iron deficiency in both vegetarian and omnivorous individuals. Compared to vegetarians, women who do not menstruate and men had the same prevalence of iron deficiency when following an omnivorous diet.
PUFA Supplementation and Heart Failure: Effects on Fibrosis and Cardiac Remodeling
Authors: Oppedisano Francesca , Mollace Rocco , Tavernese Annamaria , Gliozzi Micaela , Musolino Vincenzo , Macrì Roberta , Carresi Cristina , Maiuolo Jessica , Serra Maria , Cardamone Antonio , Volterrani Maurizio , Mollace Vincenzo
Published: August 2021

Heart failure (HF) characterized by cardiac remodeling is a condition in which inflammation and fibrosis play a key role. Dietary supplementation with n-3 polyunsaturated fatty acids (PUFAs) seems to produce good results. In fact, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have anti-inflammatory and antioxidant properties and different cardioprotective mechanisms. In particular, following their interaction with the nuclear factor erythropoietin 2 related factor 2 (NRF2), the free fatty acid receptor 4 (Ffar4) receptor, or the G-protein coupled receptor 120 (GPR120) fibroblast receptors, they inhibit cardiac fibrosis and protect the heart from HF onset. Furthermore, n-3 PUFAs increase the left ventricular ejection fraction (LVEF), reduce global longitudinal deformation, E/e ratio (early ventricular filling and early mitral annulus velocity), soluble interleukin-1 receptor-like 1 (sST2) and high-sensitive C Reactive protein (hsCRP) levels, and increase flow-mediated dilation. Moreover, lower levels of brain natriuretic peptide (BNP) and serum norepinephrine (sNE) are reported and have a positive effect on cardiac hemodynamics. In addition, they reduce cardiac remodeling and inflammation by protecting patients from HF onset after myocardial infarction (MI). The positive effects of PUFA supplementation are associated with treatment duration and a daily dosage of 1–2 g. Therefore, both the European Society of Cardiology (ESC) and the American College of Cardiology/American Heart Association (ACC/AHA) define dietary supplementation with n-3 PUFAs as an effective therapy for reducing the risk of hospitalization and death in HF patients. In this review, we seek to highlight the most recent studies related to the effect of PUFA supplementation in HF. For that purpose, a PubMed literature survey was conducted with a focus on various in vitro and in vivo studies and clinical trials from 2015 to 2021.
Changes in Gut Microbiota Induced by Doxycycline Influence in Vascular Function and Development of Hypertension in DOCA-Salt Rats
Authors: Robles-Vera Iñaki , de la Visitación Néstor , Toral Marta , Sánchez Manuel , Romero Miguel , Gómez-Guzmán Manuel , Vargas Félix , Duarte Juan , Jiménez Rosario
Published: August 2021

Previous experiments in animals and humans show that shifts in microbiota and its metabolites are linked to hypertension. The present study investigates whether doxycycline (DOX, a broad-spectrum tetracycline antibiotic) improves dysbiosis, prevent cardiovascular pathology and attenuate hypertension in deoxycorticosterone acetate (DOCA)-salt rats, a renin-independent model of hypertension. Male Wistar rats were randomly assigned to three groups: control, DOCA-salt hypertensive rats, DOCA-salt treated with DOX for 4 weeks. DOX decreased systolic blood pressure, improving endothelial dysfunction and reducing aortic oxidative stress and inflammation. DOX decreased lactate-producing bacterial population and plasma lactate levels, improved gut barrier integrity, normalized endotoxemia, plasma noradrenaline levels and restored the Treg content in aorta. These data demonstrate that DOX through direct effects on gut microbiota and its non-microbial effects (anti-inflammatory and immunomodulatory) reduces endothelial dysfunction and the increase in blood pressure in this low-renin form of hypertension.
Proteomics of Gnathostomiasis: A Way Forward for Diagnosis and Treatment Development
Authors: Thiangtrongjit Tipparat , Nogrado Kathyleen , Ketboonlue Thawatchai , Malaitong Preeyarat , Adisakwattana Poom , Reamtong Onrapak
Published: August 2021

Gnathostoma spinigerum is the most common cause of gnathostomiasis in humans. It has a complex life cycle, which requires two intermediate hosts and a definitive host, and poses a high risk for zoonosis. Definitive prognosis of gnathostomiasis relies mainly on the isolation of advanced-stage larvae (aL3), which is very challenging especially if the aL3 is sequestered in difficult-to-reach organs. There is also a lack of a confirmatory diagnostic test for gnathostomiasis. With the ongoing advancement of proteomics, a potential diagnostic approach is underway using immunoproteomics and immunodiagnostics. In addition to this, the employment of mass spectrometry could further elucidate not only understanding the biology of the parasite but also determining potential targets of prospective drugs and vaccines. This article reports the past, present, and future application of proteomics in the study of gnathostomiasis.
Tracking the Epidemiologic Shifts in Hepatitis A Sero-Prevalence Using Age Stratification: A Cross-Sectional Study at Jordan University Hospital
Authors: Kareem Nariman , Al-Salahat Khaled , Bakri Faris G. , Rayyan Yaser , Mahafzah Azmi , Sallam Malik
Published: August 2021

The study of hepatitis A virus (HAV) epidemiology and sero-prevalence has important public health implications. Changes in the epidemiology of hepatitis A can result in a larger pool of susceptible persons in countries with improved sanitation and hygienic conditions if vaccination is not provided. The aim of this study was to investigate the prevalence of HAV immunoglobulin G (IgG) in Jordan. In addition, we aimed to identify the potential differences in HAV sero-prevalence based on age, among other variables. We recruited the study participants at Jordan University Hospital in Amman, Jordan, during October 2020–June 2021. Assessment of participants’ socio-demographic variables was done using a paper-based questionnaire. Testing for HAV IgG was based on a competitive enzyme linked immunosorbent assay (ELISA). The study population comprised 360 individuals with a median age of 18 years. The overall sero-prevalence of HAV in our study sample was 38.3%. Divided by age, the sero-prevalence of HAV was 8.2%, 12.3%, and 20.8% among individuals aged 10 years or less, 15 years or less, and 30 years or less, respectively. The estimated age at mid-population immunity was between 21 and 30 years. Besides age, individuals residing outside the Central region of Jordan had a significantly higher HAV sero-prevalence. Additionally, the use of filtered municipal water was associated with a lower sero-prevalence of HAV compared with the use of unfiltered municipal water among individuals aged 15 years or less. The results of this study suggest an intermediate to low endemicity of HAV in Jordan. An epidemiologic shift of HAV sero-prevalence with a declining rate of positivity for HAV IgG was noticed in this study. This highlights the importance of the recently introduced HAV vaccination in Jordan. Future research to evaluate the public health benefits of HAV vaccination in Jordan is recommended.
Biliary Migration, Colonization, and Pathogenesis of O. viverrini Co-Infected with CagA+ Helicobacter pylori
Authors: Suyapoh Watcharapol , Tirnitz-Parker Janina E. E. , Tangkawattana Sirikachorn , Suttiprapa Sutas , Sripa Banchob
Published: August 2021

Co-infection with the cagA strain of Helicobacter pylori exacerbates the pathology of human liver fluke Opisthorchis viverrini (OV) infection leading to cholangiocarcinoma. However, underlying mechanisms remain unclear. We report a significant increase in cagA-positive and cagA-negative H. pylori in the stomach, blood, bile, and in the OV worms of co-infected Syrian golden hamsters at one hour, three hours, and one month, post-infection, compared to hamsters infected with either OV or H. pylori alone. Except in the worms, H. pylori numbers declined at three months post-infection, particularly in the bile fluid of co-infected animals. Both strains of H. pylori were immunohistochemically detected in the tegument of the worm, as well as in the bile duct epithelium when co-infected with O. viverrine, but not in H. pylori infection alone. Interestingly, only the cagA-positive strain was detected in the gut of the worm. Co-infection between cagA-positive H. pylori and O. viverrini resulted in a more severe biliary pathology and decreased E-cadherin expression in vivo and in vitro than those of the cagA-negative strain. These data suggest that O. viverrini acts as a carrier of cagA-positive H. pylori and co-migrates to the bile ducts, whereas O. viverrini facilitates H. pylori colonization and enhances the biliary pathogenesis and carcinogenesis.
JAK-STAT Pathway Regulation of Intestinal Permeability: Pathogenic Roles and Therapeutic Opportunities in Inflammatory Bowel Disease
Authors: Lei Hillmin , Crawford Meli’sa S. , McCole Declan F.
Published: August 2021

The epithelial barrier forms the interface between luminal microbes and the host immune system and is the first site of exposure to many of the environmental factors that trigger disease activity in chronic inflammatory bowel disease (IBD). Disruption of the epithelial barrier, in the form of increased intestinal permeability, is a feature of IBD and other inflammatory diseases, including celiac disease and type 1 diabetes. Variants in genes that regulate or belong to the JAK-STAT signaling pathway are associated with IBD risk. Inhibitors of the JAK-STAT pathway are now effective therapeutic options in IBD. This review will discuss emerging evidence that JAK inhibitors can be used to improve defects in intestinal permeability and how this plays a key role in resolving intestinal inflammation.
Novel Formulation of Eye Drops Containing Choline Salicylate and Hyaluronic Acid: Stability, Permeability, and Cytotoxicity Studies Using Alternative Ex Vivo and In Vitro Models
Authors: Wróblewska Katarzyna Barbara , Milanowski Bartłomiej , Kucińska Małgorzata , Plewa Szymon , Długaszewska Jolanta , Muszalska-Kolos Izabela
Published: August 2021

This work investigated the potential of a novel formulation of eye drops containing a non-steroidal anti-inflammatory drug—choline salicylate (CS)—and hyaluronic acid (HA). Thus, these drops may exert both anti-inflammatory and regenerative activity. The experiment was conducted through the careful characterization of physicochemical properties, stability, and quality of eye drops. Moreover, microbiological analysis, as well as penetration and cytotoxic studies, were performed. The UV, HPLC-UV, and HPLC-MS/MS methods were used to determine the purity and stability of CS. The penetration rate of CS was assessed using a hydrophilic membrane and ex vivo porcine cornea model. Additionally, the cytotoxic effects were evaluated using the SIRC cell line. The interaction between HA and CS was tested using size-exclusion chromatography and IR spectrophotometry. As a result, HA increased the viscosity of the drops, which prolonged their contact with the ocular surface, thus ensuring more effective penetration of CS into the corneal structure. After long-term storage, an interaction in the pharmaceutical phase between CS and HA was observed. However, this interaction did not affect the viability of rabbit corneal cells. Our findings showed that eye drops with CS and HA, stored at 2–8 °C in light-protected conditions, met the criteria of stability and safety.
Evaluation of Synthetic 2,4-Disubstituted-benzo[g]quinoxaline Derivatives as Potential Anticancer Agents
Authors: Zaki Islam , Abu El-ata Sara A. , Fayad Eman , Abu Ali Ola A. , Abu Almaaty Ali H. , Saad Ahmed S.
Published: August 2021

A new series of 2,4-disubstituted benzo[g]quinoxaline molecules have been synthesized, using naphthalene-2,3-diamine and 1,4-dibromonaphthalene-2,3-diamine as the key starting materials. The structures of the new compounds were confirmed by spectral data along with elemental microanalyses. The cytotoxic activity of all synthesized benzo[g]quinoxaline derivatives was assessed in vitro against the breast MCF-7 cancer cell line. The tested molecules revealed good cytotoxicity toward the breast MCF-7 cancer cell line, especially compound 3. The results of topoisomerase IIβ inhibition assay revealed that compound 3 exhibits potent inhibitory activity in submicromolar concentration. Additionally, compound 3 was found to cause pre-G1 apoptosis, and slightly increase the cell population at G1 and S phases of the cell cycle profile in MCF-7 cells. Finally, compound 3 induces apoptosis via Bax activation and downregulation of Bcl2, as revealed by ELISA assay.
In Vitro Interaction and Killing-Kinetics of Amphotericin B Combined with Anidulafungin or Caspofungin against Candida auris
Authors: Caballero Unai , Eraso Elena , Quindós Guillermo , Jauregizar Nerea
Published: August 2021

Treatment of invasive infections caused by Candida auris is challenging due to the limited therapeutic options. The combination of antifungal drugs may be an interesting and feasible approach to be investigated. The aim of this study was to examine the in vitro activity of amphotericin B in combination with anidulafungin or caspofungin against C. auris. In vitro static time–kill curve experiments were conducted for 48 h with different combinations of amphotericin B with anidulafungin or caspofungin against six blood isolates of C. auris. The antifungal activity of 0.5 mg/L of amphotericin B was limited against the six isolates of C. auris. Similarly, echinocandins alone had a negligible effect, even at the highest tested concentrations. By contrast, 1 mg/L of amphotericin B showed fungistatic activity. Synergy was rapidly achieved (8 h) with 0.5 mg/L of amphotericin B plus 2 mg/L of anidulafungin or caspofungin. These combinations lead to a sustained fungistatic effect, and the fungicidal endpoint was reached against some C. auris isolates. Additionally, ≥0.5 mg/L of either of the two echinocandins with 1 mg/L of amphotericin B resulted in fungicidal effect against all C. auris isolates. In conclusion, combinations of amphotericin B with anidulafungin or caspofungin provided greater killing with a lower dose requirement for amphotericin B compared to monotherapy, with synergistic and/or fungicidal outcomes.
Peptide Inhibitors of Vascular Endothelial Growth Factor A: Current Situation and Perspectives
Authors: Guryanov Ivan , Tennikova Tatiana , Urtti Arto
Published: August 2021

Vascular endothelial growth factors (VEGFs) are the family of extracellular signaling proteins involved in the processes of angiogenesis. VEGFA overexpression and altered regulation of VEGFA signaling pathways lead to pathological angiogenesis, which contributes to the progression of various diseases, such as age-related macular degeneration and cancer. Monoclonal antibodies and decoy receptors have been extensively used in the anti-angiogenic therapies for the neutralization of VEGFA. However, multiple side effects, solubility and aggregation issues, and the involvement of compensatory VEGFA-independent pro-angiogenic mechanisms limit the use of the existing VEGFA inhibitors. Short chemically synthesized VEGFA binding peptides are a promising alternative to these full-length proteins. In this review, we summarize anti-VEGFA peptides identified so far and discuss the molecular basis of their inhibitory activity to highlight their pharmacological potential as anti-angiogenic drugs.
Neoliberalism and Political Theologies of the Post-Secular: Historical, Political, and Methodological Considerations in a 20th and 21st Century Discourse
Authors: Wurts Kieryn E.
Published: August 2021

Carl Schmitt’s controversial 1922 Political Theology: Four Chapters on the Concept of Sovereignty initiated a long-standing, lively, and oft misunderstood discourse at the intersections of religious studies, theology, and political theory. Political theology as a discourse has seen something of a revival in recent decades, which has raised genuine problems of interpretation. These include questions of what is at stake in political theology, how political theology can be applied to economic discourses, and how it can be understood in relation to secularity and post-secularity. This study takes Giorgio Agamben’s The Kingdom and Glory as a conceptual bridge that helps to situate contemporary political theologies of neoliberalism historically and theoretically. A survey of four recent political theologies of neoliberalism yields a methodological reflection on the limits and potential of political theology as a discourse. A distinction is made between descriptive-genealogical political theologies and normative-prescriptive political theologies. The former is privileged in its philosophical potential, insofar as it reveals both the contingency and genuine variety of normative-prescriptive political theologies.
Identifying Streetscape Features Using VHR Imagery and Deep Learning Applications
Authors: Verma Deepank , Mumm Olaf , Carlow Vanessa Miriam
Published: August 2021

Deep Learning (DL) based identification and detection of elements in urban spaces through Earth Observation (EO) datasets have been widely researched and discussed. Such studies have developed state-of-the-art methods to map urban features like building footprint or roads in detail. This study delves deeper into combining multiple such studies to identify fine-grained urban features which define streetscapes. Specifically, the research focuses on employing object detection and semantic segmentation models and other computer vision methods to identify ten streetscape features such as movement corridors, roadways, sidewalks, bike paths, on-street parking, vehicles, trees, vegetation, road markings, and buildings. The training data for identifying and classifying all the elements except road markings are collected from open sources and finetuned to fit the study’s context. The training dataset is manually created and employed to delineate road markings. Apart from the model-specific evaluation on the test-set of the data, the study creates its own test dataset from the study area to analyze these models’ performance. The outputs from these models are further integrated to develop a geospatial dataset, which is additionally utilized to generate 3D views and street cross-sections for the city. The trained models and data sources are discussed in the research and are made available for urban researchers to exploit.
Relationship between TIR and NIR-SWIR as Indicator of Vegetation Water Availability
Authors: Holzman Mauro , Rivas Raúl , Bayala Martín
Published: August 2021

Water availability for vegetation use has been associated with the relative amount of water in the plant and is a key factor for modeling variables related to the soil-plant system (e.g., net primary production, drought effects on vegetation). To the best of our knowledge, the integration of spectral proxies of vegetation water content (near-infrared (NIR), shortwave-infrared (SWIR) bands) and land surface temperature (LST) for estimation, not only of vegetation water content but also soil water available for the evapotranspiration process requires more analysis. This study aims to assess the relationship between NIR, SWIR reflectance, and LST data as indicators of water availability for crop use. For this purpose, vegetation water content, LST, and spectral reflectance over soybean, corn, and barley were measured in the field and the laboratory. Based on the consistency of satellite data from Moderate-Resolution Imaging Spectroradiometer (MODIS/Aqua) in relation to such measurements, a model is proposed, which can be parameterized from remotely sensed NIR-SWIR/LST scatterplots. The obtained results were tested in the Argentine Pampas, showing coherence with surface processes at regional scale associated with soil water availability. The comparison with soil moisture at different depths (R2 > 0.7) showed that the method is sensitive to variations in root zone water availability. Given the reliance of the index on just satellite data, it can be pointed that the potential not only for vegetation water stress analyses but also in the context of hydrological modeling as an input of water availability.
The Ongoing Greening in Southwest China despite Severe Droughts and Drying Trends
Authors: Chen Xin , Chen Tiexi , Yan Qingyun , Cai Jiangtao , Guo Renjie , Gao Miaoni , Wei Xueqiong , Zhou Shengjie , Li Chaofan , Xie Yong
Published: August 2021

Vegetation greening, which refers to the interannual increasing trends of vegetation greenness, has been widely found on the regional to global scale. Meanwhile, climate extremes, especially several drought, significantly damage vegetation. The Southwest China (SWC) region experienced massive drought from 2009 to 2012, which severely damaged vegetation and had a huge impact on agricultural systems and life. However, whether these extremes have significantly influenced long-term (multiple decades) vegetation change is unclear. Using the latest remote sensing-based records, including leaf area index (LAI) and gross primary productivity (GPP) for 1982–2016 and enhanced vegetation index (EVI) for 2001–2019, drought events of 2009–2012 only leveled off the greening (increasing in vegetation indices and GPP) temporally and long-term greening was maintained. Meanwhile, drying trends were found to unexpectedly coexist with greening.
A Novel Slip Sensory System for Interfacial Condition Monitoring of Steel-Concrete Composite Bridges
Authors: Sadeghi Faraz , Zhu Xinqun , Li Jianchun , Rashidi Maria
Published: August 2021

Steel-concrete composite (SCC) beams are widely employed in bridge decks. The interfacial shear transfer between the top concrete slab and the supporting steel beams significantly affects the overall load carrying capacity and performance of a bridge deck. The inaccessibility of the connection system makes the visual inspection difficult, and the traditional vibration-based methods are insensitive to this type of local damage. In this study, a novel interlayer slip monitoring system has been developed for interfacial condition assessment of SCC beams. The monitoring system is mainly based on the Ultra-flat Industrial Potentiometer Membrane (UIPM). The sensor film that is glued on a steel base is mounted on the concrete slab, and the wiper is installed on the steel beam. The interlayer slip between the concrete slab and steel beam is monitored by the relative displacement between the sensor film and the wiper. An experimental study has been carried out on a 6-m long composite bridge model in the laboratory. In the model, the concrete slab and the steel beams are bolt-connected, and the bolts could be loosened to simulate the defects in the shear connection system. Seven slip sensors are evenly installed along the bridge model. The sensors are calibrated using the testing machine before they are installed on the bridge model. Three damage scenarios are simulated by loosening bolts at different locations. Different loadings are also applied on the bridge to simulate the operational conditions. Undamaged and damaged scenarios have been considered within load increments, and data are collected and interpreted to find out how the slip changes. The results show that this system is reliable and efficient to monitor the interlayer slip for assessing the interface condition of composite structures.
Understanding and Acceptance of Smart City Policies: Practitioners’ Perspectives on the Malaysian Smart City Framework
Authors: Lim Seng Boon , Malek Jalaluddin Abdul , Yussoff Md Farabi Yussoff Md , Yigitcanlar Tan
Published: August 2021

Whilst a plethora of research exists on the smart cities and project performance evaluations, only few studies have focused on the smart city policy evaluation from the perspective of its acceptance by practitioners. This paper aims to generate insights by evaluating the smart city policy through a developing country case study—i.e., Malaysia. This study employed a questionnaire survey method for data collection and analyzed the data by using Fuzzy Delphi analysis. A group of 40 practitioners was gathered in a focus group discussion through purposive sampling. The main objectives of this survey were to identify the understanding and acceptance levels of the seven smart city domains and respective strategies that are outlined in the Malaysian Smart City Framework. The results disclosed that the practitioners possessed divergent levels of understanding and acceptance in terms of smart city domains. The study participant practitioners accepted all understanding and acceptance objectives of smart economy, living, people, and governance domains (expert agreement 75–92% and threshold d value 0.123–0.188), but rejected all objectives for both smart environment and digital infrastructure domains (expert agreement 55–74% and threshold d value 0.150–0.212). Along with this, acceptance of smart mobility was also rejected (expert agreement 56% and threshold d value 0.245). The findings reveal that considering all opinions expressing dissensus is essential when building more inclusive smart city strategies. This study contributes to the smart city discourse as being one of the first in capturing professional practitioners’ understanding and acceptance on a national level smart city policy by applying the Delphi method in the smart city context. Most importantly, the study informs urban policymakers on how to capture the voices and perspectives of the general public on national and local smart city strategy and initiatives.