bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and validation of a deep learning model for breast lesion segmentation and characterization in multiparametric MRI
Authors: Zhu Jingjin , Geng Jiahui , Shan Wei , Zhang Boya , Shen Huaqing , Dong Xiaohan , Liu Mei , Li Xiru , Cheng Liuquan
Published: August 2022

Importance

The utilization of artificial intelligence for the differentiation of benign and malignant breast lesions in multiparametric MRI (mpMRI) assists radiologists to improve diagnostic performance.

Objectives

To develop an automated deep learning model for breast lesion segmentation and characterization and to evaluate the characterization performance of AI models and radiologists.

Materials and methods

For lesion segmentation, 2,823 patients were used for the training, validation, and testing of the VNet-based segmentation models, and the average Dice similarity coefficient (DSC) between the manual segmentation by radiologists and the mask generated by VNet was calculated. For lesion characterization, 3,303 female patients with 3,607 pathologically confirmed lesions (2,213 malignant and 1,394 benign lesions) were used for the three ResNet-based characterization models (two single-input and one multi-input models). Histopathology was used as the diagnostic criterion standard to assess the characterization performance of the AI models and the BI-RADS categorized by the radiologists, in terms of sensitivity, specificity, accuracy, and the area under the receiver operating characteristic curve (AUC). An additional 123 patients with 136 lesions (81 malignant and 55 benign lesions) from another institution were available for external testing.

Results

Of the 5,811 patients included in the study, the mean age was 46.14 (range 11–89) years. In the segmentation task, a DSC of 0.860 was obtained between the VNet-generated mask and manual segmentation by radiologists. In the characterization task, the AUCs of the multi-input and the other two single-input models were 0.927, 0.821, and 0.795, respectively. Compared to the single-input DWI or DCE model, the multi-input DCE and DWI model obtained a significant increase in sensitivity, specificity, and accuracy (0.831 vs. 0.772/0.776, 0.874 vs. 0.630/0.709, 0.846 vs. 0.721/0.752). Furthermore, the specificity of the multi-input model was higher than that of the radiologists, whether using BI-RADS category 3 or 4 as a cutoff point (0.874 vs. 0.404/0.841), and the accuracy was intermediate between the two assessment methods (0.846 vs. 0.773/0.882). For the external testing, the performance of the three models remained robust with AUCs of 0.812, 0.831, and 0.885, respectively.

Conclusions

Combining DCE with DWI was superior to applying a single sequence for breast lesion characterization. The deep learning computer-aided diagnosis (CADx) model we developed significantly improved specificity and achieved comparable accuracy to the radiologists with promise for clinical application to provide preliminary diagnoses.
Identification of methylation signatures associated with CAR T cell in B-cell acute lymphoblastic leukemia and non-hodgkin’s lymphoma
Authors: Song Jiwei , Huang FeiMing , Chen Lei , Feng KaiYan , Jian Fangfang , Huang Tao , Cai Yu-Dong
Published: August 2022

CD19-targeted CAR T cell immunotherapy has exceptional efficacy for the treatment of B-cell malignancies. B-cell acute lymphocytic leukemia and non-Hodgkin’s lymphoma are two common B-cell malignancies with high recurrence rate and are refractory to cure. Although CAR T-cell immunotherapy overcomes the limitations of conventional treatments for such malignancies, failure of treatment and tumor recurrence remain common. In this study, we searched for important methylation signatures to differentiate CAR-transduced and untransduced T cells from patients with acute lymphoblastic leukemia and non-Hodgkin’s lymphoma. First, we used three feature ranking methods, namely, Monte Carlo feature selection, light gradient boosting machine, and least absolute shrinkage and selection operator, to rank all methylation features in order of their importance. Then, the incremental feature selection method was adopted to construct efficient classifiers and filter the optimal feature subsets. Some important methylated genes, namely, SERPINB6, ANK1, PDCD5, DAPK2, and DNAJB6, were identified. Furthermore, the classification rules for distinguishing different classes were established, which can precisely describe the role of methylation features in the classification. Overall, we applied advanced machine learning approaches to the high-throughput data, investigating the mechanism of CAR T cells to establish the theoretical foundation for modifying CAR T cells.
Case report: Congenital hyperinsulinemia with ABCC8 gene mutations
Authors: Zhang Jin , Wang Jiyang , Chen Hui
Published: August 2022

Background

Congenital hyperinsulinemia (CHI) is an inherited disease of abnormal insulin secretion and is the main cause of persistent and intractable hypoglycemia in infants. The aim of this case report was to investigate the genetic mechanisms and treatment of CHI in an affected patient.

Case summary

We collected clinical data from, and performed gene capture, high-throughput gene sequencing analysis, and Sanger sequencing validation, in a child with CHI and his family to identify the causative gene mutations. Two heterozygous pathogenic mutations in the ATP-binding cassette subfamily C member 8 (ABCC8) gene were detected in the child: c.863G>A (p.Trp288Ter) in exon 6 and c.2506C>T (p.Arg836Ter) in exon 21. Sanger sequencing showed that c.863G>A was inherited from heterozygous mutations in the paternal line and c.2506C>T from heterozygous mutations in the maternal line.

Conclusion

The child was a CHI with a biallelic recessive heterozygous mutations in ABCC8 resulting in impairment of its encoded ATP-sensitive potassium (KATP) channel, poor response to diazoxide treatment, and developed diabetes after subtotal pancreatectomy.
Outcomes and comparations of pediatric surgery about choledochal cyst with robot-assisted procedures, laparoscopic procedures, and open procedures: A meta-analysis
Authors: Xie Siqi , Huang Yanbing , He Yuanbin , Liu Mingkun , Wu Dianming , Fang Yifan
Published: August 2022

Background

Choledochal cysts (CC) are rare disorders characterized by congenital biliary dilatation of the intrahepatic or extrahepatic bile ducts and always relate to pancreaticobiliary maljunction. Robot-assisted surgery has been able to complete almost all pediatric endoscopic surgery nowadays. But evidence of the post-operative outcomes of robotic-assisted operation is limited, comparing with the laparoscopic operation and traditional open operation. The aim of this meta-analysis was to identify the advantages and deficiencies about robotic-assisted operation for CC.

Methods

A meta-analysis of retrospective studies published in PUBMED, MEDLINE, Web of Science and China National Knowledge Infrastructure (CNKI). No date limit was used, with the last search on April 30, 2022. No publication restrictions or study design filters were applied.

Results

Nine retrospective cohort studies with 1,395 patients [366 in the robotic-assisted operation group (RG), 532 in the laparoscopic operation group (LG) and 497 in the open operation group (OG)] were enrolled in our study. Subgroup analysis demonstrated the RG had significant longer operative time [standardized mean difference (SMD) = 1.59, 95% CI = (0.02, 3.16), P < 0.05], less blood loss [SMD = −1.52, 95% CI = (−2.71, −0.32), P < 0.05], shorter enteral feeding time [SMD = −0.83, 95% CI = (−1.22, −0.44), P < 0.001], shorter time to stay in the hospital [SMD = −0.81, 95% CI = (−1.23, −0.38), P < 0.001], fewer post-operative complications [Relative risk (RR) =1.09, 95% CI = (1.04, 1.13), P < 0.001] but higher expenses [SMD = 8.58, 95% CI = (5.27, 11.89), P < 0.001] than LG. While a significant older age [SMD = 0.46, 95% CI = (0.26, 0.66), P < 0.001], longer operative time [SMD = 3.96, 95% CI = (2.38, 5.55), P < 0.001] and shorter time to stay in the hospital [SMD = −0.93, 95% CI = (−1.62, −0.25), P < 0.05] than OG.

Conclusions

Laparoscopic and robotic-assisted procedure are both safe and minimal invasive operational strategies. Robotic-assisted procedure may slowly surpass and has a trend to replace laparoscopy for its advantages. More experiences in robotic-assisted operation should be accumulated for the unexpected complexities, so as to be more stable in the younger age of children.
Inhibiting Cytoprotective Autophagy in Cancer Therapy: An Update on Pharmacological Small-Molecule Compounds
Authors: Zhang Lijuan , Zhu Yuxuan , Zhang Jiahui , Zhang Lan , Chen Lu
Published: August 2022

Autophagy is a self-degradation process in which damaged proteins and organelles are engulfed into autophagosomes for digestion and eventually recycled for cellular metabolism to maintain intracellular homeostasis. Accumulating studies have reported that autophagy has the Janus role in cancer as a tumor suppressor or an oncogenic role to promote the growth of established tumors and developing drug resistance. Importantly, cytoprotective autophagy plays a prominent role in many types of human cancers, thus inhibiting autophagy, and has been regarded as a promising therapeutic strategy for cancer therapy. Here, we focus on summarizing small-molecule compounds inhibiting the autophagy process, as well as further discuss other dual-target small-molecule compounds, combination strategies, and other strategies to improve potential cancer therapy. Therefore, these findings will shed new light on exploiting more small-molecule compounds inhibiting cytoprotective autophagy as candidate drugs for fighting human cancers in the future.
Taccalonolides: Structure, semi-synthesis, and biological activity
Authors: Li Yan , Du Yu-Feng , Gao Feng , Xu Jin-Bu , Zheng Ling-Li , Liu Gang , Lei Yu
Published: August 2022

Microtubules are the fundamental part of the cell cytoskeleton intimately involving in cell proliferation and are superb targets in clinical cancer therapy today. Microtubule stabilizers have become one of the effectively main agents in the last decades for the treatment of diverse cancers. Taccalonolides, the highly oxygenated pentacyclic steroids isolated from the genus of Tacca, are considered a class of novel microtubule-stabilizing agents. Taccalonolides not only possess a similar microtubule-stabilizing activity as the famous drug paclitaxel but also reverse the multi-drug resistance of paclitaxel and epothilone in cellular and animal models. Taccalonolides have captured numerous attention in the field of medicinal chemistry due to their variety of structures, unique mechanism of action, and low toxicity. This review focuses on the structural diversity, semi-synthesis, modification, and pharmacological activities of taccalonolides, providing bright thoughts for the discovery of microtubule-stabilizing drugs.
Endothelitis profile in acute heart failure and cardiogenic shock patients: Endocan as a potential novel biomarker and putative therapeutic target
Authors: Reina-Couto Marta , Silva-Pereira Carolina , Pereira-Terra Patrícia , Quelhas-Santos Janete , Bessa João , Serrão Paula , Afonso Joana , Martins Sandra , Dias Cláudia Camila , Morato Manuela , Guimarães João T , Roncon-Albuquerque Roberto , Paiva José-Artur , Albino-Teixeira António , Sousa Teresa
Published: August 2022

Aims: Inflammation-driven endothelitis seems to be a hallmark of acute heart failure (AHF) and cardiogenic shock (CS). Endocan, a soluble proteoglycan secreted by the activated endothelium, contributes to inflammation and endothelial dysfunction, but has been scarcely explored in human AHF. We aimed to evaluate serum (S-Endocan) and urinary endocan (U-Endocan) profiles in AHF and CS patients and to correlate them with biomarkers/parameters of inflammation, endothelial activation, cardiovascular dysfunction and prognosis.

Methods: Blood and spot urine were collected from patients with AHF (n = 23) or CS (n = 25) at days 1–2 (admission), 3-4 and 5-8 and from controls (blood donors, n = 22) at a single time point. S-Endocan, U-Endocan, serum IL-1β, IL-6, tumour necrosis factor-α (S-TNF-α), intercellular adhesion molecule-1 (S-ICAM-1), vascular cell adhesion molecule-1 (S-VCAM-1) and E-selectin were determined by ELISA or multiplex immunoassays. Serum C-reactive protein (S-CRP), plasma B-type natriuretic peptide (P-BNP) and high-sensitivity troponin I (P-hs-trop I), lactate, urea, creatinine and urinary proteins, as well as prognostic scores (APACHE II, SAPS II) and echocardiographic left ventricular ejection fraction (LVEF) were also evaluated.

Results: Admission S-Endocan was higher in both patient groups, with CS presenting greater values than AHF (AHF and CS vs. Controls, p < 0.001; CS vs. AHF, p < 0.01). Admission U-Endocan was only higher in CS patients (p < 0.01 vs. Controls). At admission, S-VCAM-1, S-IL-6 and S-TNF-α were also higher in both patient groups but there were no differences in S-E-selectin and S-IL-1β among the groups, nor in P-BNP, S-CRP or renal function between AHF and CS. Neither endocan nor other endothelial and inflammatory markers were reduced during hospitalization (p > 0.05). S-Endocan positively correlated with S-VCAM-1, S-IL-6, S-CRP, APACHE II and SAPS II scores and was positively associated with P-BNP in multivariate analyses. Admission S-Endocan raised in line with LVEF impairment (p = 0.008 for linear trend).

Conclusion: Admission endocan significantly increases across AHF spectrum. The lack of reduction in endothelial and inflammatory markers throughout hospitalization suggests a perpetuation of endothelial dysfunction and inflammation. S-Endocan appears to be a biomarker of endothelitis and a putative therapeutic target in AHF and CS, given its association with LVEF impairment and P-BNP and its positive correlation with prognostic scores.
Identification of single major QTL and candidate gene(s) governing hull-less seed trait in pumpkin
Authors: Kaur Barinder , Garcha Karmvir Singh , Bhatia Dharminder , Khosa Jiffinvir Singh , Sharma Madhu , Mittal Amandeep , Verma Neha , Dhatt Ajmer Singh
Published: August 2022

The hull-less pumpkin (Cucurbita pepo) seed does not require de-hulling before use for human consumption, as a result highly preferred by the oil, nut, and baking industries. In hull-less seeds, a single recessive gene is responsible for the absence of outer thick seed coat layers; however, the genomic region and gene(s) controlling the trait are unclear to date. In this study, four crosses attempted to derive F2 and backcross populations confirmed the single recessive gene inheritance of hull-less seed trait in populations adapted to the sub-tropical climate. The candidate genomic region for hull-less seed trait was identified through the BSA-QTLseq approach using bulks of F2:3 progenies from a cross of HP111 (hulled) and HLP36 (hull-less). A novel genomic region on chromosome 12 ranging from 1.80 to 3.86 Mb was associated with the hull-less seed trait. The re-sequencing data identified a total of 396 SNPs within this region and eight were successfully converted into polymorphic KASP assays. The genotyping of segregating F2 (n = 160) with polymorphic KASP assays resulted in a 40.3 cM partial linkage map and identified Cp_3430407 (10 cM) and Cp_3498687 (16.1 cM) as flanking markers for hull-less locus (Cphl-1). These flanking markers correspond to the 68.28 kb region in the reference genome, and the marker, Cp_3430407 successfully predicted the genotype in 93.33% of the C. pepo hull-less germplasm lines, thus can be used for marker-assisted selection in parents polymorphic for the hull-less seed trait. The Cphl-1-linked genomic region (2.06 Mb) encompasses a total of 182 genes, including secondary cell wall and lignin biosynthesis-related transcriptional factors viz., “NAC” (Cp4.1LG12g04350) and “MYB” (Cp4.1LG12g03120). These genes were differentially expressed in the seeds of hulled and hull-less genotypes, and therefore could be the potential candidate genes governing the hull-less seed trait in pumpkin.
An intelligent monitoring system of diseases and pests on rice canopy
Authors: Li Suxuan , Feng Zelin , Yang Baojun , Li Hang , Liao Fubing , Gao Yufan , Liu Shuhua , Tang Jian , Yao Qing
Published: August 2022

Accurate and timely surveys of rice diseases and pests are important to control them and prevent the reduction of rice yields. The current manual survey method of rice diseases and pests is time-consuming, laborious, highly subjective and difficult to trace historical data. To address these issues, we developed an intelligent monitoring system for detecting and identifying the disease and pest lesions on the rice canopy. The system mainly includes a network camera, an intelligent detection model of diseases and pests on rice canopy, a web client and a server. Each camera of the system can collect rice images in about 310 m2 of paddy fields. An improved model YOLO-Diseases and Pests Detection (YOLO-DPD) was proposed to detect three lesions of Cnaphalocrocis medinalis, Chilo suppressalis, and Ustilaginoidea virens on rice canopy. The residual feature augmentation method was used to narrow the semantic gap between different scale features of rice disease and pest images. The convolution block attention module was added into the backbone network to enhance the regional disease and pest features for suppressing the background noises. Our experiments demonstrated that the improved model YOLO-DPD could detect three species of disease and pest lesions on rice canopy at different image scales with an average precision of 92.24, 87.35 and 90.74%, respectively, and a mean average precision of 90.11%. Compared to RetinaNet, Faster R-CNN and Yolov4 models, the mean average precision of YOLO-DPD increased by 18.20, 6.98, 6.10%, respectively. The average detection time of each image is 47 ms. Our system has the advantages of unattended operation, high detection precision, objective results, and data traceability.
Indolamine accumulation and TDC/T5H expression profiles reveal the complex and dynamic regulation of serotonin biosynthesis in tomato (Solanum lycopersicum L.)
Authors: Commisso Mauro , Negri Stefano , Gecchele Elisa , Fazion Emanuela , Pontoriero Cecilia , Avesani Linda , Guzzo Flavia
Published: August 2022

Tryptamine and serotonin are indolamines that fulfill diverse biological functions in all kingdoms of life. Plants convert l-tryptophan into tryptamine and then serotonin via consecutive decarboxylation and hydroxylation reactions catalyzed by the enzymes tryptophan decarboxylase (TDC) and tryptamine 5-hydroxylase (T5H). Tryptamine and serotonin accumulate to high levels in the edible fruits and seeds of many plant species, but their biological roles in reproductive organs remain unclear and the metabolic pathways have not been characterized in detail. We identified three TDC genes and a single T5H gene in tomato (Solanum lycopersicum L.) by homology-based screening and confirmed their activity by heterologous expression in Nicotiana benthamiana. The co-analysis of targeted metabolomics and gene expression data revealed complex spatiotemporal gene expression and metabolite accumulation patterns that suggest the involvement of the serotonin pathway in multiple biological processes. Our data support a model in which SlTDC1 allows tryptamine to accumulate in fruits, SlTDC2 causes serotonin to accumulate in aerial vegetative organs, and SlTDC3 works with SlT5H to convert tryptamine into serotonin in the roots and fruits.
Conceptualising the separation from an abusive partner as a multifactorial, non-linear, dynamic process: A parallel with Newton’s laws of motion
Authors: Di Basilio Daniela , Guglielmucci Fanny , Livanou Maria
Published: August 2022

The present study focused on the dynamics and factors underpinning domestic abuse (DA) survivors’ decisions to end the abusive relationship. The experiences and opinions of 12 female DA survivors and 18 support workers were examined through in-depth, one-to-one, semi-structured interviews. Hybrid thematic analysis was conducted to retrieve semantic themes and explore relationships among the themes identified and the differences in survivors’ and professionals’ narratives of the separation process. The findings highlighted that separation decisions derived from the joint action of two sets of factors, the “promoters” and the “accelerators.” Whilst the “promoters” are factors leading to the separation from the abuser over time, the “accelerators” bear a stronger and more direct connection with survivors’ decision to end the abusive relationship. Despite their differences, both these factors acted as propelling forces, leading survivors to actively pursue the separation from the perpetrator. To portray the dynamic links among these factors, we propose a conceptualisation drawn from Newton’s laws of motion. Our findings also highlighted important differences in the views of survivors and support workers, as the former conceived themselves as proactive in ending the abuse, whereas the latter described the leaving process as mainly led by authorities and services supporting survivors. This study has potential implications for research, policy and clinical practice, as it suggests that far from being a linear sequence of multiple stages, leaving an abusive relationship results from a complex interplay of factors that facilitate (“promoters”) or drastically accelerate (“accelerators”) the separation process. We argue that future research should aim at improving our current understanding of the subjective and situational factors that can act as “accelerators” or “promoters” for women’s leaving decisions. Moreover, clinicians and policymakers should invest in creating interventions that aid victims to recognise and leverage promoters and accelerators, thus increasing their readiness to end the abuse.
Leader’s strategies for designing the promotional path of regional brand competitiveness in the context of economic globalization
Authors: Li Pei , Du Jianguo , Shahzad Fakhar
Published: August 2022

In the era of economic globalization, the competitiveness of products on a global scale is increasingly achieved through effective and sustainable strategies for brand development by the leaders. This paper conducts an empirical study on regional brand competitiveness (BC) influencing factors. A research model was proposed and tested by employing structural equation modeling. Data analysis was conducted using 214 valid questionnaires from two major producing areas in Jilin Province, China. Research results show that Brand Market (BM) and Government Guidance (GG) directly and positively impact the regional BC. Regional Resource (RR) and industrial development (ID) indirectly impact the regional BC through the mediating role of BM and GG. BM is the most important factor affecting the regional BC. Based on this, the path to improve the competitiveness of traditional agricultural products under economic globalization is determined, and targeted countermeasures and suggestions are formulated for the existing problems.
The cost-effectiveness of digital health interventions: A systematic review of the literature
Authors: Gentili Andrea , Failla Giovanna , Melnyk Andriy , Puleo Valeria , Tanna Gian Luca Di , Ricciardi Walter , Cascini Fidelia
Published: August 2022

Background

Digital health interventions have significant potential to improve safety, efficacy, and quality of care, reducing waste in healthcare costs. Despite these premises, the evidence regarding cost and effectiveness of digital tools in health is scarce and limited.

Objectives

The aim of this systematic review is to summarize the evidence on the cost-effectiveness of digital health interventions and to assess whether the studies meet the established quality criteria.

Methods

We queried PubMed, Scopus and Web of Science databases for articles in English published from January 1, 2016 to December 31, 2020 that performed economic evaluations of digital health technologies. The methodological rigorousness of studies was assessed with the Consolidated Health Economic Evaluation Reporting Standards (CHEERS). The review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) 2009 checklist.

Results

Search identified 1,476 results, 552 of which were selected for abstract and 35 were included in this review. The studies were heterogeneous by country (mostly conducted in upper and upper-middle income countries), type of eHealth intervention, method of implementation, and reporting perspectives. The qualitative analysis identified the economic and effectiveness evaluation of six different types of interventions: (1) seventeen studies on new video-monitoring service systems; (2) five studies on text messaging interventions; (3) five studies on web platforms and digital health portals; (4) two studies on telephone support; (5) three studies on new mobile phone-based systems and applications; and (6) three studies on digital technologies and innovations.

Conclusion

Findings on cost-effectiveness of digital interventions showed a growing body of evidence and suggested a generally favorable effect in terms of costs and health outcomes. However, due to the heterogeneity across study methods, the comparison between interventions still remains difficult. Further research based on a standardized approach is needed in order to methodically analyze incremental cost-effectiveness ratios, costs, and health benefits.
Barriers and facilitators of rehabilitation nursing care for patients with disability in the rehabilitation hospital: A qualitative study
Authors: Shirozhan Shima , Arsalani Narges , Seyed Bagher Maddah Sadat , Mohammadi-Shahboulaghi Farahnaz
Published: August 2022

Nurses play a key role in providing rehabilitation care. In this regard, identifying the factors that affect their practice can be useful in planning to improve the quality of rehabilitation nursing care. This study aims to explore the experience of nurses and members of the rehabilitation team about barriers and facilitators of rehabilitation nursing care of patients with disability in the rehabilitation hospital. This qualitative study was conducted in the main public rehabilitation hospital in Tehran, Iran. Eighteen persons including 12 nurses in clinical and managerial positions, an occupational therapist, a physical medicine specialist, a patient, and an informal caregiver participated in this study. Participants were selected based on purposeful sampling. Data were collected through 18 in-depth semi-structured interviews and analyzed based on qualitative content analysis principles. Three themes were derived from the data analysis, which represented Barriers and facilitators related to nurses (specialized knowledge and skills, psychological status, mentoring, professional communication), barriers and facilitators related to the work environment (nurses' performance evaluation, nursing workforce, comprehensive care facilities, workplace design, specialized unit), barriers and facilitators related to patients and caregivers (patient's participation in nursing care, patient adaptation, efficiency of formal caregivers). The experiences of the rehabilitation team shows that not only nurses, but also the environment, patients, and caregivers can affect the provision of care and change the quality of care. Identifying these factors can help managers, researchers, and clinical nurses to facilitate and improve rehabilitation nursing care by modifying the influencing factors.
The psychological process and emotional cognition of children's tourism experiences in Chinese family culture
Authors: Guo Xuanxuan , Liu Tao
Published: August 2022

The family cultural environment affects children's cognitive development and socialization processes, and different family cultural environments lead to differences in children's tourism experiences. The current research on children's tourism experiences demands a shift from the families' perspective to that of children's perspective. In response to this, grounded on the cognitive development theory, this paper, from the perspective of children's memorable parent-child tourism experience, uses 321 children's drawings to project the tourism elements, people, activities, scenes, and colors that children perceive from travel, reveals the main cognitive contents of children's parent-child tourism experiences. Furthermore, this paper analyzes the influence of family cultural background on children's tourism experiences through interviews with children. Our results show that with the growth of age, children's perception of elements changes from the macro level to the micro level, and the contents they perceive change from concrete to abstract. In addition, children have an acute perception of people and are impressed by novel activities during travel. They adapt well to changes in travel scenes and prefer bright and vibrant colors during trips. Therefore, we recommend the design of appropriate tourism products that combine the characteristics of children's experiences when offering parent-child travel programs, as well as upgrading the market of parent-child tourism experiences through novel activities.
Sustainability of breastfeeding interventions to reduce child mortality rates in low, middle-income countries: A systematic review of randomized controlled trials
Authors: Engelhart Alexis , Mason Stacey , Nwaozuru Ucheoma , Obiezu-Umeh Chisom , Carter Victoria , Shato Thembekile , Gbaja-Biamila Titilola , Oladele David , Iwelunmor Juliet
Published: August 2022

Child mortality is the lowest it has ever been, but the burden of death in low- and middle-income countries (LMICs) is still prevalent, and the numbers average above the global mean. Breastfeeding contributes to the reduction of child mortality by improving chance of survival beyond childhood. Therefore, it is essential to examine how evidence-based breastfeeding interventions are being maintained in resource-constrained settings. Guided by Scheirer and Dearing's sustainability framework, the aim of this systematic review was to explore how evidence-based breastfeeding interventions implemented to address child mortality in LMICs are sustained. The literature search included randomized controlled trials (RCTs) of breastfeeding interventions from the following electronic databases: Cochrane Library, Global Health, PubMed, Scopus, and Web of Science. Literature selection and data extraction were completed according to the PRISMA guidelines. A narrative synthesis was used to investigate factors that contributed to sustainability failure or success. A total of 497 articles were identified through the database search. Only three papers were included in the review after the removal of duplicates and assessment for eligibility. The three RCTs included breastfeeding interventions predominately focusing on breastfeeding initiation and exclusivity in rural, semi-rural, and peri-urban areas in South Africa, Kenya, and India. The number of women included in the studies ranged from 901 to 3,890, and the duration of studies stretched from 6 weeks to 2.5 years. In two studies, sustainability was reported as the continuation of the intervention, and the other study outlined program dissemination and scale-up. Facilitators and barriers that influenced the sustainability of breastfeeding interventions were largely related to specific characteristics of the interventions (i.e., strong intervention implementers—facilitator; small number of CHWs involved—barrier). Optimizing the sustainability of breastfeeding interventions in LMICs is imperative to reduce child mortality. The focal point of implementation must be planning for sustainability to lead to continued benefits and changes in population outcomes. A defined action plan for sustainability needs to be included in both funding and research.
Recent advances and opportunities in data assimilation for physics-based hydrological modeling
Authors: Camporese Matteo , Girotto Manuela
Published: August 2022

Data assimilation applications in integrated surface-subsurface hydrological models (ISSHMs) are generally limited to scales ranging from the hillslope to local or meso-scale catchments. This is because ISSHMs resolve hydrological processes in detail and in a physics-based fashion and therefore typically require intensive computational efforts and rely on ground-based observations with a small spatial support. At the other end of the spectrum, there is a vast body of literature on remote sensing data assimilation for land surface models (LSMs) at the continental or even global scale. In LSMs, some hydrological processes are usually represented with a coarse resolution and in empirical ways, especially groundwater lateral flows, which may be very important and yet often neglected. Starting from the review of some recent progress in data assimilation for physics-based hydrological models at multiple scales, we stress the need to find a common ground between ISSHMs and LSMs and suggest possible ways forward to advance the use of data assimilation in integrated hydrological models.
Pre-Pandemic Adversity Buffers the Role of Social Loneliness in Caregiver Mental Health During the COVID-19 Pandemic
Authors: Monahan Emma Kahle , McCrae Julie S. , Daferede Simeon
Published: August 2022

Objectives: This study investigates how family profiles of risk and resilience established prior to COVID-19 are associated with changes in caregiver depression and stress 1 year after the pandemic onset, and how these associations are moderated by experiences of social loneliness.

Methods: A sample of 243 caregivers in four risk and resilience profiles interviewed pre-COVID-19 were interviewed virtually in December 2020–February 2021 (during pandemic). Multi-level models were used to examine changes in mental health.

Results: All caregivers reported increases in extreme stress during the pandemic. Caregivers with less relative adversity pre-pandemic showed significantly greater depression and loneliness in the pandemic compared to caregivers with higher pre-pandemic adversity. Social loneliness was a moderator of the association between pre-pandemic adversity and mental health.

Conclusion: The study suggests families with more pre-pandemic adversity demonstrate coping that buffers the negative impact of social loneliness on mental health, emphasizing the strengths of these families that are assets to build upon in crisis. Families with more relative advantage pre-pandemic likely need assistance to reduce feelings of stress and depression in the face of increased social loneliness.
Identification and Validation of an m6A-Related LncRNA Signature to Predict Progression-Free Survival in Colorectal Cancer
Authors: Zhang Yong , Li Lu , Chu Feifei , Xiao Xingguo , Zhang Li , Li Kunkun , Wu Huili
Published: August 2022

The RNA methylation of N6 adenosine (m6A) plays a crucial role in various biological processes. Strong evidence reveals that the dysregulation of long non-coding RNAs (lncRNA) brings about the abnormality of downstream signaling in multiple ways, thus influencing tumor initiation and progression. Currently, it is essential to discover effective and succinct molecular biomarkers for predicting colorectal cancer (CRC) prognosis. However, the prognostic value of m6A-related lncRNAs for CRC remains unclear, especially for progression-free survival (PFS). Here, we screened 24 m6A-related lncRNAs in 622 CRC patients and identified five lncRNAs (SLCO4A1-AS1, MELTF-AS1, SH3PXD2A-AS1, H19 and PCAT6) associated with patient PFS. Compared to normal samples, their expression was up-regulated in CRC tumors from TCGA dataset, which was validated in 55 CRC patients from our in-house cohort. We established an m6A-Lnc signature for predicting patient PFS, which was an independent prognostic factor by classification analysis of clinicopathologic features. Moreover, the signature was validated in 1,077 patients from six independent datasets (GSE17538, GSE39582, GSE33113, GSE31595, GSE29621, and GSE17536), and it showed better performance than three known lncRNA signatures for predicting PFS. In summary, our study demonstrates that the m6A-Lnc signature is a promising biomarker for forecasting patient PFS in CRC.
Post-Mortem Dental Profile as a Powerful Tool in Animal Forensic Investigations—A Review
Authors: Viciano Joan , López-Lázaro Sandra , Tanga Carmen
Published: August 2022

Veterinary forensics is becoming more important in our society as a result of the growing demand for investigations related to crimes against animals or investigations of criminal deaths caused by animals. A veterinarian may participate as an expert witness or may be required to give forensic assistance, by providing knowledge of the specialty to establish a complete picture of the involvement of an animal and allowing the Courts to reach a verdict. By applying diverse dental profiling techniques, not only can species, sex, age-at-death, and body size of an animal be estimated, but also data about their geographical origin (provenance) and the post-mortem interval. This review concentrates on the dental techniques that use the characteristics of teeth as a means of identification of freshly deceased and skeletonised animals. Furthermore, this highlights the information that can be extracted about the animal from the post-mortem dental profile.
The Ionospheric Responses from Satellite Observations within Middle Latitudes to the Strong Magnetic Storm on 25–26 August 2018
Authors: Zhang Xuemin , Dong Lei , Nie Lei
Published: August 2022

The multi observations from the China Seismo-Electromagnetic Satellite (CSES) were presented and analyzed during the biggest magnetic storm on 25–26 August in the quiet solar activity year of 2018, together with the Swarm satellite and GNSS TEC (Global Navigation Satellite System, Total Electron Content). The whole tempo-spatial evolutional process was demonstrated in electromagnetic fields and in-situ plasma parameters within the whole magnetic storm time period of three phases, the main phase with quick decrease in SYM-H, the quick recovery phase, and the slow recovery phase. Strong correlations were revealed in time and space between electric fields and electron density. During the main phase, the penetrated electric field was the major factor to induce the injection of electric fields to low latitudes even to the equator and contribute to constructing the double peaks of Ne at altitudes above 500 km of CSES in daytime. In the quick recovery phase, Ne depletion was found in low middle and low latitudes in the daytime, associated with a quick decrease in solar wind dynamic pressure, but in the nightside Ne maintained or increased. Due to the high solar wind speed following the quick recovery phase, it controlled the enhancements in an electric field below 1125 Hz at medium and low latitudes in daytime and produced similar structures in a 225 Hz electric field with the mid-latitude trough of Ne in local nighttime and maintained their equator-ward movements in this time period. Ne/TEC showed typical local time-dependence in this magnetic storm, which illustrated that although the electron density in the ionosphere was mainly caused by this solar activity event, local background environments must also not be ignored for their final evolutional modes.
Visuomotor Tracking Task for Enhancing Activity in Motor Areas of Stroke Patients
Authors: Wasaka Toshiaki , Ando Kohei , Nomura Masakazu , Toshima Kazuya , Tamaru Tsukasa , Morita Yoshifumi
Published: August 2022

Recovery of motor function following stroke requires interventions to enhance ipsilesional cortical activity. To improve finger motor function following stroke, we developed a movement task with visuomotor feedback and measured changes in motor cortex activity by electroencephalography. Stroke patients performed two types of movement task on separate days using the paretic fingers: a visuomotor tracking task requiring the patient to match a target muscle force pattern with ongoing feedback and a simple finger flexion/extension task without feedback. Movement-related cortical potentials (MRCPs) were recorded before and after the two motor interventions. The amplitudes of MRCPs measured from the ipsilesional hemisphere were significantly enhanced after the visuomotor tracking task but were unchanged by the simple manual movement task. Increased MRCP amplitude preceding movement onset revealed that the control of manual movement using visual feedback acted on the preparatory stage from motor planning to execution. A visuomotor tracking task can enhance motor cortex activity following a brief motor intervention, suggesting efficient induction of use-dependent cortical plasticity in stroke.
The Role of Conization before Radical Hysterectomy in Cervical Cancer including High Risk Factors of Recurrence: Propensity Score Matching
Authors: Chang Chi-Son , Min Ji Song , Song Ki Hyeon , Choi Chel Hun , Kim Tae-Joong , Lee Jeong-Won , Kim Byoung-Gie , Lee Yoo-Young
Published: August 2022

We primarily aimed to investigate the therapeutic role of conization prior to radical hysterectomy for cervical cancer. Secondarily, we aimed to characterize a subgroup of patients who could potentially benefit from preoperative conization. Patients who underwent radical hysterectomy for FIGO 2009 stage IB1 to IIB cervical cancer from 1995 to 2020 were eligible. The patients were divided into two groups: those with and without preoperative conization. To adjust for the baseline characteristics of the two groups, 1:2 case–control propensity score matching was conducted. Survival analysis was performed between the two groups. Subgroup analysis was performed to identify the effect of conization based on clinical and pathological factors. Patients who underwent preoperative conization showed better 5-year overall survival than those who did not (95.9% vs. 93.0%, p = 0.029); however, no difference was observed in progression-free survival (88.9% vs. 85.9%, p = 0.155). In multivariate Cox analysis, conization showed a 55% reduction in recurrence (hazard ratio (HR) 0.65, 95% confidence interval (CI) 0.41–1.01, p = 0.056) and 41% reduction in death (HR 0.59, 95% CI 0.34–1.02, p = 0.059), but with marginal statistical significance. In subgroup analysis, minimally invasive surgery (MIS), negative pelvic lymph node, and tumor size < 4 cm showed improved survival from conization. Conization before radical hysterectomy may be associated with improved survival in patients with early-stage cervical cancer. This information could serve as a basis for a more tailored patient selection for MIS for cervical cancer.
Pseudocontinuous Arterial Spin Labeling: Clinical Applications and Usefulness in Head and Neck Entities
Authors: Tanaka Fumine , Umino Maki , Maeda Masayuki , Nakayama Ryohei , Inoue Katsuhiro , Kogue Ryota , Obara Makoto , Sakuma Hajime
Published: August 2022

As functional magnetic resonance imaging, arterial spin labeling (ASL) techniques have been developed to provide quantitative tissue blood flow measurements, which can improve the performance of lesion diagnosis. ASL does not require contrast agents, thus, it can be applied to a variety of patients regardless of renal impairments and contrast agent allergic reactions. The clinical implementation of head and neck lesions is limited, although, in recent years, ASL has been increasingly utilized in brain lesions. Here, we review the development of the ASL techniques, including pseudocontinuous ASL (pCASL). We compare readout methods between three-dimensional (3D) turbo spin-echo and 2D echo planar pCASL for the clinical applications of pCASL to head and neck lesions. We demonstrate the clinical usefulness of 3D pCASL for diagnosing various entities, including inflammatory lesions, hypervascular lesions, and neoplasms; for evaluating squamous cell carcinoma (SCC) treatment responses, and for predicting SCC prognosis.
Higher Rate of Lymphedema with Inguinal versus Axillary Complete Lymph Node Dissection for Melanoma: A Potential Target for Immediate Lymphatic Reconstruction?
Authors: Deban Melina , Vallance Patrick , Jost Evan , McKinnon J. Gregory , Temple-Oberle Claire
Published: August 2022

Background: The present study was conducted to define the lymphedema rate at our institution in patients undergoing axillary (ALND) or inguinal (ILND) lymph node dissection (LND) for melanoma. It aimed to examine risk factors predisposing patients to a higher rate of lymphedema, highlighting which patients could be targeted for immediate lymphatic reconstruction (ILR). Methods: A retrospective chart review was conducted between October 2015 and July 2020 to identify patients who had undergone ALND or ILND for melanoma. The main outcome measures were rates of transient and permanent lymphedema. Univariate and multivariate analyses were performed to assess the relationship between lymphedema rate and factors related to patient characteristics, surgical procedure, pathology findings, and adjuvant treatment. Results: Between October 2015 and July 2020, 66 patients underwent LND for melanoma: 34 patients underwent ALND and 32 patients underwent ILND. At a median follow-up of 29 months, 85.3% (n = 29) of patients having had an ALND did not experience lymphedema, versus 50.0% (n = 16) of ILND (p = 0.0019). The rates of permanent lymphedema for patients having undergone ALND and ILND were 11.8% (n = 4) and 37.5% (n = 12) respectively (p = 0.016, NS). The rate of transient lymphedema was 2.9% (n = 1) for ALND and 12.5% (n = 4) for ILND (p = 0.13, NS). On univariate analysis, the location of LND and wound infection were found to be significant factors for lymphedema. On multivariate analysis, only the location of LND remained a significant predictor, with the inguinal location predisposing to lymphedema. Conclusion: This study highlights the high rate of lymphedema following ILND for melanoma and is a potential target for future patients to be considered for ILR.
Sustainable Biofuel Production from Animal Manure and Crop Residues in Ghana
Authors: Seglah Patience , Wang Yajing , Wang Hongyan , Gao Chunyu , Bi Yuyun
Published: August 2022

Renewable energy is predicted to play a crucial role in the energy sector as transitioning from fossil fuels to biofuels is key to achieving carbon neutrality. The comprehensive utilization of livestock manure and crop residues as a viable source of biofuels can play a significant role in Ghana’s future energy system. This current study aims to examine and predict the biofuel potential in Ghana from animal manure and crop residues. The biogas-biomethane potential of animal manure is determined using anaerobic digestion technology, whereas crop residues’ bioethanol potential is assessed using computational and analytical approaches. The findings demonstrate that animal manure biogas potential is 237.72 normal million cubic meters (NMm3). The highest manure-biogas potential per animal is from cattle (27.15 Nm3) and pigs (18.15 Nm3), while the least contribution is from chickens (1.80 Nm3). In addition, the annual bioethanol production is 3.74 gigaliters (GL), and residues from maize (0.88 GL), yams (0.82 GL), and plantains (0.47 GL) had the largest share per annum. By 2100, Ghana has the potential to produce 1288.56 NMm3 of manure-based biogas which can offset 13.88% of liquefied petroleum gas (LPG) consumption. The biogas potential positively correlates with the biomethane potential of 901.99 NMm3. Furthermore, 91.85% of the 14.76 GL of bioethanol will be from food crop residues. This study provides critical information to lawmakers and energy planners with strategies to achieve energy security and minimize the ecological footprint in an optimistic scenario.
Energy Monitoring Technologies
Authors: Quintal Filipe
Published: August 2022

Energy monitoring is a vast field of research [...]