bluuebunny/crossref_metadata_2025 huggingface dataset:
Co-Ultramicronized Palmitoylethanolamide/Luteolin-Induced Oligodendrocyte Precursor Cell Differentiation is Associated With Tyro3 Receptor Upregulation
Authors: Facci Laura , Barbierato Massimo , Fusco Mariella , Giusti Pietro , Zusso Morena
Published: June 2021

Remyelination in patients with multiple sclerosis frequently fails, especially in the chronic phase of the disease promoting axonal and neuronal degeneration and progressive disease disability. Drug-based therapies able to promote endogenous remyelination capability of oligodendrocytes are thus emerging as primary approaches to multiple sclerosis. We have recently reported that the co-ultramicronized composite of palmitoylethanolamide and the flavonoid luteolin (PEALut) promotes oligodendrocyte precursor cell (OPC) maturation without affecting proliferation. Since TAM receptor signaling has been reported to be important modulator of oligodendrocyte survival, we here evaluated the eventual involvement of TAM receptors in PEALut-induced OPC maturation. The mRNAs related to TAM receptors -Tyro3, Axl, and Mertk- were all present at day 2 in vitro. However, while Tyro3 gene expression significantly increased upon cell differentiation, Axl and Mertk did not change during the first week in vitro. Tyro3 gene expression developmental pattern resembled that of MBP myelin protein. In OPCs treated with PEALut the developmental increase of Tyro3 mRNA was significantly higher as compared to vehicle while was reduced gene expression related to Axl and Mertk. Rapamycin, an inhibitor of mTOR, prevented oligodendrocyte growth differentiation and myelination. PEALut, administered to the cultures 30 min after rapamycin, prevented the alteration of mRNA basal expression of the TAM receptors as well as the expression of myelin proteins MBP and CNPase. Altogether, data obtained confirm that PEALut promotes oligodendrocyte differentiation as shown by the increase of MBP and CNPase and Tyro3 mRNAs as well as CNPase and Tyro3 immunostainings. The finding that these effects are reduced when OPCs are exposed to rapamycin suggests an involvement of mTOR signaling in PEALut effects.
Opening the Schrödinger Box: Short- and Long-Range Mammalian Heart Rate Variability
Authors: Weiser-Bitoun Ido , Davoodi Moran , Rosenberg Aviv A. , Alexandrovich Alexandra , Yaniv Yael
Published: June 2021

Background

The interactions between the autonomic nervous system (ANS), intrinsic systems (e.g., endocrine), and internal pacemaker mechanisms govern short (milliseconds–seconds)- and long (seconds–minutes)-range heart rate variability (HRV). However, there is a debate regarding the identity of the mechanism underlying HRV on each time scale. We aim to design a general method that accurately differentiates between the relative contribution of the ANS and pacemaker mechanisms to HRV in various mammals, without the need for drug perturbations or organ isolation. Additionally, we aim to explore the universality of the relative contribution of the ANS and pacemaker system of different mammals.

Methods

This work explored short- and long-range HRVs using published ECG data from dogs, rabbits, and mice. To isolate the effects of ANS on HRV, ECG segments recorded before and after ANS-blockade were compared.

Results

Differentiation of the ANS from extrinsic and intrinsic pacemaker mechanisms was successfully achieved. In dogs, the internal pacemaker mechanisms were the main contributors to long-range and the ANS to short-range HRV. In rabbits and mice, the ANS and the internal pacemaker mechanisms affected both time scales, and anesthesia changed the relative contribution of the pacemaker mechanism to short- and long-range HRVs. In mice, the extrinsic mechanisms affected long-range HRV, while their effect was negligible in rabbits.

Conclusion

We offer a novel approach to determine the relative contributions of ANS and extrinsic and intrinsic pacemaker mechanisms to HRV and highlight the importance of selecting mammalian research models with HRV mechanisms representative of the target species of interest.
Lactate Fluxes and Plasticity of Adipose Tissues: A Redox Perspective
Authors: Lagarde Damien , Jeanson Yannick , Portais Jean-Charles , Galinier Anne , Ader Isabelle , Casteilla Louis , Carrière Audrey
Published: June 2021

Lactate, a metabolite produced when the glycolytic flux exceeds mitochondrial oxidative capacities, is now viewed as a critical regulator of metabolism by acting as both a carbon and electron carrier and a signaling molecule between cells and tissues. In recent years, increasing evidence report its key role in white, beige, and brown adipose tissue biology, and highlights new mechanisms by which lactate participates in the maintenance of whole-body energy homeostasis. Lactate displays a wide range of biological effects in adipose cells not only through its binding to the membrane receptor but also through its transport and the subsequent effect on intracellular metabolism notably on redox balance. This study explores how lactate regulates adipocyte metabolism and plasticity by balancing intracellular redox state and by regulating specific signaling pathways. We also emphasized the contribution of adipose tissues to the regulation of systemic lactate metabolism, their roles in redox homeostasis, and related putative physiopathological repercussions associated with their decline in metabolic diseases and aging.
The Plastid-Encoded RNA Polymerase-Associated Protein PAP9 Is a Superoxide Dismutase With Unusual Structural Features
Authors: Favier Adrien , Gans Pierre , Boeri Erba Elisabetta , Signor Luca , Muthukumar Soumiya Sankari , Pfannschmidt Thomas , Blanvillain Robert , Cobessi David
Published: June 2021

In Angiosperms, the plastid-encoded RNA polymerase (PEP) is a multimeric enzyme, essential for the proper expression of the plastid genome during chloroplast biogenesis. It is especially required for the light initiated expression of photosynthesis genes and the subsequent build-up of the photosynthetic apparatus. The PEP complex is composed of a prokaryotic-type core of four plastid-encoded subunits and 12 nuclear-encoded PEP-associated proteins (PAPs). Among them, there are two iron superoxide dismutases, FSD2/PAP9 and FSD3/PAP4. Superoxide dismutases usually are soluble enzymes not bound into larger protein complexes. To investigate this unusual feature, we characterized PAP9 using molecular genetics, fluorescence microscopy, mass spectrometry, X-ray diffraction, and solution-state NMR. Despite the presence of a predicted nuclear localization signal within the sequence of the predicted chloroplast transit peptide, PAP9 was mainly observed within plastids. Mass spectrometry experiments with the recombinant Arabidopsis PAP9 suggested that monomers and dimers of PAP9 could be associated to the PEP complex. In crystals, PAP9 occurred as a dimeric enzyme that displayed a similar fold to that of the FeSODs or manganese SOD (MnSODs). A zinc ion, instead of the expected iron, was found to be penta-coordinated with a trigonal-bipyramidal geometry in the catalytic center of the recombinant protein. The metal coordination involves a water molecule and highly conserved residues in FeSODs. Solution-state NMR and DOSY experiments revealed an unfolded C-terminal 34 amino-acid stretch in the stand-alone protein and few internal residues interacting with the rest of the protein. We hypothesize that this C-terminal extension had appeared during evolution as a distinct feature of the FSD2/PAP9 targeting it to the PEP complex. Close vicinity to the transcriptional apparatus may allow for the protection against the strongly oxidizing aerial environment during plant conquering of terrestrial habitats.
Metabolomics Analysis in Different Development Stages on SP0 Generation of Rice Seeds After Spaceflight
Authors: Zeng Deyong , Cui Jie , Yin YiShu , Xiong Yi , Liu Mengyao , Guan Shuanghong , Cheng Dayou , Sun Yeqing , Lu Weihong
Published: June 2021

Spaceflight is a special abiotic stress condition. In recent years, it has been confirmed that the spaceflight caused the stress response of rice seeds, and the protein level, transcription level, and methylation level will change during the planting process after returning to the ground. However, the changes at the metabolome level are not very clear. In this study, two kinds of rice seeds, Dongnong423 (DN3) and Dongnong416 (DN6), were carried on the ShiJian-10 retractable satellite (SJ-10) for 12.5 days in orbit, returned to the ground and planted in the field until the three-leaf (TLP) and tillering stage (TS). The results of antioxidant enzyme activity, soluble sugar, and electron leakage rate revealed that the spaceflight caused the stress response of rice. The TLP and TS of DN3 identified 110 and 57 different metabolites, respectively, while the TLP and TS of DN6 identified 104 and 74 different metabolites, respectively. These metabolites included amino acids, sugars, fatty acids, organic acids and secondary metabolites. We used qRT-PCR technology to explore the changes of enzyme genes in the tricarboxylic acid cycle (TCA) and amino acid metabolism pathway. Combined with the results of metabolomics, we determined that during the TLP, the TCA cycle rate of DN3 was inhibited and amino acid metabolism was activated, while the TCA cycle rate of DN6 was activated and amino acid metabolism was inhibited. In TS, the TCA cycle rate of DN3 was inhibited, and amino acid metabolism was not significantly changed, while the TCA cycle rate of DN6 was activated and amino acid metabolism was inhibited. These results suggested that the response mechanisms of the two different rice strains to spaceflight stress are different, and these differences may be reflected in energy consumption and compound biosynthesis of rice in different growth and development stages. This study provided new insights for further exploring the effects of spaceflight.
Physical Activity and Life Satisfaction: An Empirical Study in a Population of Senior Citizens
Authors: Wöbbeking Sánchez Marina , Sánchez Cabaco Antonio , Bonete-López Beatriz , Urchaga Litago José David , Loureiro Manuel Joaquím , Mejía Manuel
Published: June 2021

Objective: The specialised literature indicates that the two key aspects in active ageing are performing physical activity and life satisfaction. Regarding physical activity, this not only improves physical aspects of senior citizens, but also has a positive impact on mental well-being and satisfaction with one’s own life. The aim is to demonstrate the relationship between these two variables to explain healthy ageing.

Method: In a sample of 300 senior citizen subjects, the influence of various sociodemographic variables (age, sex, institutionalisation, and level of education) on the performance of physical activity and life satisfaction, is analysed. The research design is a non-experimental study with two unique cross-sectional and correlational measurement groups.

Results: An analysis of the results indicates that people with a higher level of education present differences in physical and motivational reserves. Furthermore, age and institutionalisation have an impact on physical reserves. Analysis using structural equation models allows key relationships between the variables analysed to be predicted, which can guide the implementation of active ageing.

Conclusion: Motivational reserves affect healthy cognitive ageing through their positive impact on cognitive and physical reserves.
Why Do People (Not) Engage in Social Distancing? Proximate and Ultimate Analyses of Norm-Following During the COVID-19 Pandemic
Authors: Norton James O. , Evans Kortnee C. , Semchenko Ayten Yesim , Al-Shawaf Laith , Lewis David M. G.
Published: June 2021

COVID-19 has had a profound negative effect on many aspects of human life. While pharmacological solutions are being developed and implemented, the onus of mitigating the impact of the virus falls, in part, on individual citizens and their adherence to public health guidelines. However, promoting adherence to these guidelines has proven challenging. There is a pressing need to understand the factors that influence people’s adherence to these guidelines in order to improve public compliance. To this end, the current study investigated whether people’s perceptions of others’ adherence predict their own adherence. We also investigated whether any influence of perceived social norms was mediated by perceptions of the moral wrongness of non-adherence, anticipated shame for non-adherence, or perceptions of disease severity. One hundred fifty-two Australians participated in our study between June 6, 2020 and August 21, 2020. Findings from this preliminary investigation suggest that (1) people match their behavior to perceived social norms, and (2) this is driven, at least in part, by people using others’ behavior as a cue to the severity of disease threat. Such findings provide insight into the proximate and ultimate bases of norm-following behavior, and shed preliminary light on public health-related behavior in the context of a pandemic. Although further research is needed, the results of this study—which suggest that people use others’ behavior as a cue to how serious the pandemic is and as a guide for their own behavior—could have important implications for public health organizations, social movements, and political leaders and the role they play in the fight against epidemics and pandemics.
Germaphobia! Does Our Relationship With and Knowledge of Biodiversity Affect Our Attitudes Toward Microbes?
Authors: Robinson Jake M. , Cameron Ross , Jorgensen Anna
Published: June 2021

Germaphobia – a pathological aversion to microorganisms – could be contributing to an explosion in human immune-related disordersviamass sterilization of surfaces and reduced exposure to biodiversity. Loss of biodiversity and people’s weaker connection to nature, along with poor microbial literacy may be augmenting the negative consequences of germaphobia on ecosystem health. In this study, we created an online questionnaire to acquire data on attitudes toward, and knowledge of microbes. We collected data on nature connectedness and interactions with nature and explored the relationships between these variables. Although the study had an international reach (n= 1,184), the majority of responses came from England, United Kingdom (n= 993). We found a significant association between attitudes toward microbes and both duration and frequency of visits to natural environments. A higher frequency of visits to nature per week, and a longer duration spent in nature per visit, was significantly associated with positive attitudes toward microbes. We found no association between nature connectedness and attitudes toward microbes. We found a significant relationship between knowledge of “lesser known” microbial groups (e.g., identifying that fungi, algae, protozoa, and archaea are microbes) and positive attitudes toward microbes. However, we also found that people who identified viruses as being microbes expressed less positive views of microbes overall–this could potentially be attributed to a “COVID-19 effect.” Our results suggest that basic microbial literacy and nature engagement may be important in reducing/preventing germaphobia-associated attitudes. The results also suggest that a virus-centric phenomenon (e.g., COVID-19) could increase broader germaphobia-associated attitudes. As the rise of immune-related disorders and mental health conditions have been linked to germaphobia, reduced biodiversity, and non-targeted sterilization, our findings point to a feasible strategy to potentially help ameliorate these negative consequences. Further research is needed, but greater emphasis on microbial literacy and promoting time spent in nature could potentially be useful in promoting resilience in human health and more positive/constructive attitudes toward the foundations of our ecosystems—the microorganisms.
Unchanged Cognitive Performance and Concurrent Prefrontal Blood Oxygenation After Accelerated Intermittent Theta-Burst Stimulation in Depression: A Sham-Controlled Study
Authors: Struckmann Wiebke , Persson Jonas , Gingnell Malin , Weigl Wojciech , Wass Caroline , Bodén Robert
Published: June 2021

Aim: Intermittent theta-burst stimulation (iTBS) delivered over the dorsomedial prefrontal cortex (DMPFC) has shown promise as a treatment for anhedonia and amotivation in patients with depression. Here, we investigated whether this protocol modulates cognitive performance and concurrent prefrontal blood oxygenation. We also examined whether depressed patients exhibit cognitive dysfunction and prefrontal hypoactivity at baseline compared to healthy controls.

Methods: This sham-controlled study comprises 52 patients randomized to either active or sham accelerated iTBS over the DMPFC (applied twice daily) for 10 consecutive treatment days, and 55 healthy controls. Cognitive performance was assessed at baseline and once again 4 weeks later using a cognitive test battery targeting attention, inhibitory control, and numerical, verbal, and visual working memory. Concurrent prefrontal oxygenated hemoglobin (oxy-Hb) was captured with functional near-infrared spectroscopy.

Results: Active iTBS over DMPFC did not affect cognitive performance or concurrent oxy-Hb change compared to sham iTBS in patients with depression. Compared to controls, patients at baseline showed impaired performance in the Trail Making Test, the Rey Auditory Verbal Learning Test, the Animal Naming Test, and the Digit Symbol Substitution Test, however no difference in prefrontal oxy-Hb was observed.

Conclusion: Patients with treatment-resistant depression displayed cognitive deficits, however without prefrontal hypoactivity, compared to healthy controls at baseline. iTBS treatment did not alter cognitive performance, nor concurrent prefrontal blood oxygenation, in patients. Taken together, iTBS can likely be considered a cognitively safe treatment option in this sample of patients.
Difference in Response to Feedback and Gender in Three Therapeutic Community Units
Authors: Warren Keith , Doogan Nathan J. , Doherty Fiona
Published: June 2021

Therapeutic communities (TCs) for substance abuse incorporate a system of peer feedback through written affirmations and corrections. Previous research has found that TC residents show a response to affirmations that is detectable for roughly 8 weeks, with response to corrections being of shorter duration and weaker overall. It is not clear whether and to what extent response to feedback in TCs varies between men and women. Previous research in other settings suggests that women should be more responsive to feedback than men. In order to test this hypothesis we draw on a large dataset of affirmations and corrections sent and received in three 80 bed TC units, two of which house men and one of which houses women. The analysis uses a multilevel negative binomial model, treating affirmations and corrections that TC residents receive as predictors of affirmations that they send over a 9 week period (week 0, the week during which affirmations and corrections are actually sent, and eight subsequent weeks). The model controls for gender, age, race, unit and scores on the Level of Service Inventory-Revised (LSI-R). The relationship between affirmations received and those sent is stronger for women during the initial week and on lags 1-2 and 5-8. The relationship between corrections received and affirmations sent is stronger for women on lags 2 and 8. Graphs suggest that response to affirmations falls off in an exponential curve, while that to corrections appears to include a periodic element. These results indicate that both men and women respond to feedback, but that the strength of the women's response is somewhat greater. These results suggest that any difference in suitability by gender to the feedback approach that characterizes TCs may favor women.
Machine Teaching for Human Inverse Reinforcement Learning
Authors: Lee Michael S. , Admoni Henny , Simmons Reid
Published: June 2021

As robots continue to acquire useful skills, their ability to teach their expertise will provide humans the two-fold benefit of learning from robots and collaborating fluently with them. For example, robot tutors could teach handwriting to individual students and delivery robots could convey their navigation conventions to better coordinate with nearby human workers. Because humans naturally communicate their behaviors through selective demonstrations, and comprehend others’ through reasoning that resembles inverse reinforcement learning (IRL), we propose a method of teaching humans based on demonstrations that are informative for IRL. But unlike prior work that optimizes solely for IRL, this paper incorporates various human teaching strategies (e.g. scaffolding, simplicity, pattern discovery, and testing) to better accommodate human learners. We assess our method with user studies and find that our measure of test difficulty corresponds well with human performance and confidence, and also find that favoring simplicity and pattern discovery increases human performance on difficult tests. However, we did not find a strong effect for our method of scaffolding, revealing shortcomings that indicate clear directions for future work.
Distracted? Blame Your Claustrum!
Authors: Fatal Yonatan , Citri Ami
Published: June 2021

To succeed in reading these sentences, your brain must ignore a variety of distractions—sights, sounds, and smells. Resisting distractions is a vital ability for our daily lives, and it poses a unique challenge for people who are dealing with attention disorders. In a recent study, we asked whether an area in the brain called the claustrum supports the ability to ignore distractions. We developed methods that allowed us to silence the claustrum in mice. We challenged these mice with tasks requiring them to pay attention. We found that mice whose claustra were silenced were especially sensitive to distractions. These results provide an important clue about the function of the claustrum, and we hope that they will contribute to the development of new methods that will assist people dealing with attention disorders.
Choice of Microbial System for In-Situ Resource Utilization on Mars
Authors: Averesch Nils Jonathan Helmuth
Published: June 2021

Various microbial systems have been explored for their applicability to in-situ resource utilisation (ISRU) on Mars and suitability to leverage Martian resources and convert them into useful chemical products. Considering only fully bio-based solutions, two approaches can be distinguished, which comes down to the form of carbon that is being utilized: (a) the deployment of specialised species that can directly convert inorganic carbon (atmospheric CO2) into a target compound or (b) a two-step process that relies on independent fixation of carbon and the subsequent conversion of biomass and/or complex substrates into a target compound. Due to the great variety of microbial metabolism, especially in conjunction with chemical support-processes, a definite classification is often difficult. This can be expanded to the forms of nitrogen and energy that are available as input for a biomanufacturing platform. To provide a perspective on microbial cell factories that may be suitable for Space Systems Bioengineering, a high-level comparison of different approaches is conducted, specifically regarding advantages that may help to extend an early human foothold on the red planet.
Erratum: Torra et al. Amaranthus palmeri a New Invasive Weed in Spain with Herbicide Resistant Biotypes. Agronomy 2020, 10, 993
Authors: Torra Joel , Royo-Esnal Aritz , Romano Yolanda , Osuna María Dolores , León Ramón G. , Recasens Jordi
Published: June 2021

The authors would like to make the following correction to the published paper [...]
Efficacy of the Nitrification Inhibitor 3,4 Dimethylpyrazol Succinic Acid (DMPSA) when Combined with Calcium Ammonium Nitrate and Ammonium Sulphate—A Soil Incubation Experiment
Authors: Rahman Niharika , Henke Catarina , Forrestal Patrick J.
Published: June 2021

The efficacy of the new nitrification inhibitor 3,4 dimethylpyrazol succinic acid (DMPSA) was tested with calcium ammonium nitrate (CAN) and ammonium sulphate (AS) fertilisers in an incubation experiment using a sandy loam soil and a sandy textured soil. The experiment was conducted over 80 days. For AS fertiliser, inclusion of DMPSA resulted in significantly less NO3−-N present after 19 days in both soils. In the case of CAN, inclusion of DMPSA resulted in significantly less NO3−-N present after 45 days in the sandy loam soil and after 30 days in the sandy soil. DMPSA is effective nitrification inhibitor when combined with CAN and AS, with a mean reduction of 61% and 58%, respectively, in the average daily nitrification rate over the study period. Over the 80-day incubation period in the sandy loam soil, only 35% NH4+-N was converted to NO3−-N for AS + DMPSA compared to 88% for AS. In the sandy soil, 92% NH4+-N was converted to NO3−-N for AS compared with only 9% for AS + DMPSA by day 80. The results demonstrate that DMPSA is an effective nitrification inhibitor when combined with CAN and AS.
Photosynthesis, Biomass Production, Nutritional Quality, and Flavor-Related Phytochemical Properties of Hydroponic-Grown Arugula (Eruca sativa Mill.) ‘Standard’ under Different Electrical Conductivities of Nutrient Solution
Authors: Yang Teng , Samarakoon Uttara , Altland James , Ling Peter
Published: June 2021

Arugula (Eruca sativa) is cultivated using hydroponic techniques in greenhouses to fulfill high year-round demand, but its nutrient management in hydroponic production has not yet been standardized, potentially leading to limited quality and productivity. Aiming to address this issue, we investigated the effect of electrical conductivity (EC) on yield, nutritional and phytochemical properties of arugula. The model cultivar arugula ‘Standard’ was grown at four different EC levels (1.2, 1.5, 1.8, and 2.1 dS·m−1). Our results indicated photosynthetic properties, SPAD, leaf area, yield and dry weight increased with increasing EC from 1.2 to 1.8 dS·m−1. Foliar nutrient content increased with higher EC, but nutrient solution with 2.1 dS·m−1 showed a significant decline in N, Ca and most of the micronutrients including Fe, Zn, Mo, Cu, B and Mn. Total glucosinolates, total chlorophyll and total carotenoids concentrations increased with increasing EC. In addition, total anthocyanin content was highest in plants grown in EC 1.2 and 2.1 dS·m−1, demonstrating a stress response when grown in extreme EC levels. Our results further indicated a rapid accumulation of nitrate with higher EC, potentially detrimental to human health. This research demonstrated the optimal EC range would be 1.5 to 1.8 dS·m−1 for arugula in hydroponic production systems based on yield, quality criteria and human health considerations.
Ultrasound-Guided Lateral Transversus Abdominis Plane (TAP) Block in Rabbits: A Cadaveric Study
Authors: Di Bella Caterina , Pennasilico Luca , Staffieri Francesco , Serino Federica , Palumbo Piccionello Angela
Published: June 2021

The aims of the study were to describe the ultrasonographic-guided lateral TAP block in rabbit cadavers and evaluate the spread of a lidocaine/methylene blue solution through a single fascial infiltration. The US-guided block and anatomical dissections were performed in 17 New Zealand rabbit cadavers. The probe was placed perpendicular to the column, one centimetre ventrally to the transverse processes, halfway between the iliac crest and the costal margin. External oblique (EO), internal oblique (IO), and transversus abdominis (TA) muscles were visualised, and 1 mL/kg of lidocaine 2% plus methylene blue 1% was injected. After dissection, the branches of spinal nerves stained were measured. Moreover, the percentage of length and height of the area marked were calculated. A good visualisation of the TAP was obtained in all 34 hemiabdomens. T11 nerve eminence was successfully stained in 52% of cases. T12, L1, and L2 were stained in 75%, 95%, and 100% of cases, respectively. L3 and L4 were stained in 60% and 40% of cases, respectively. The lateral TAP block with a single point of injection can be easily performed in rabbits, but it is not sufficient to cover the nerve eminences of the cranial abdomen. The two-point TAP block (lateral and subcostal) could represent a better option, particularly when large surgical incisions are required.
Students’ Attitudes to and Knowledge of Brown Bears (Ursus arctos L.): Can More Knowledge Reduce Fear and Assist in Conservation Efforts?
Authors: Oražem Vesna , Smolej Tadeja , Tomažič Iztok
Published: June 2021

The expansion of large carnivores across Europe is posing a challenge to their conservation. Since success with conservation may depend significantly on human behavior, knowledge of certain behaviors’ emergence and all the factors that affect them are crucial. The present study included 534 students who were divided into a comparison group (n = 317) and a treatment group (n = 217) consisting of 309 lower secondary (LS, MAge = 12.2, SD = 0.94) and 225 upper secondary (US, n = 225, MAge = 16.5, SD = 0.99) school students. We assessed their attitudes to and knowledge of brown bears. An indirect effect of the workshops (instructions) is also described. Sociodemographic factors, such as gender and seeing a bear in nature, significantly influenced the students’ attitudes and knowledge. Residence, owning a dog, having a hunter in the family, breeding livestock and visiting a zoo had a smaller effect on the students’ attitudes and knowledge. The results thus show that greater knowledge is correlated with proconservation attitudes, and partly with reduction of fear. Therefore, future conservation and management should employ strong communication, especially education activities based on direct experiences and carefully designed information regarding species and socio-scientific issues.
The Effect of Feeding Management and Culling of Cows on the Lactation Curves and Milk Production of Primiparous Dairy Cows
Authors: Różańska-Zawieja Jolanta , Winnicki Stanisław , Zyprych-Walczak Joanna , Szabelska-Beręsewicz Alicja , Siatkowski Idzi , Nowak Włodzimierz , Stefańska Barbara , Kujawiak Ryszard , Sobek Zbigniew
Published: June 2021

The study attempted to estimate the lactation curves of primiparous dairy cows in relation to their feeding management. Therefore, the first aim of the study was to determine and compare the lactation curves of primiparous dairy cows using Wood’s model and to estimate the association between the lactation curves and feeding management. The second objective was to investigate the effect of the culling rate on improvement in the milk yield of primiparous dairy herds. The study was conducted on four commercial dairy farms of Polish Holstein–Friesian cows using different feeding systems (TMR—total mixed ration and PMR—partial mixed ration) and management (T1—one TMR throughout lactation; P1—one PMR throughout lactation; T2 and T3—three feed periods such as FRESH, TMR I and TMR II according to days in milk). The data used for the study were obtained from monthly milk performance evaluations of 1662 primiparous cows conducted by the Polish Federation of Cattle Breeding and Dairy Farmers throughout the year 2015. Wood’s lactation model was used to plot curves for milk yield, fat and protein content, lactose content, and milk urea contents. The highest milk yield for the whole lactation and in the peak lactation phase was recorded for cows in herd T1. This herd reached peak lactation on day 105 of milking, with an average milk yield of 42.1 kg, which was about 5 kg more milk than in the other herds. The study showed that the culling of primiparous cows in herd T1 after 30, 60 and 90 days of lactation prevented a significant reduction in milk yield in a 305-day lactation. It also increased average milk production by 1586.9 kg per primiparous dairy cow.
Effect of Aging on the Quality of Breast Meat from Broilers Affected by Wooden Breast Myopathy
Authors: Oliveira Rodrigo Fortunato de , Mello Juliana Lolli Malagoli de , Ferrari Fábio Borba , Souza Rodrigo Alves de , Pereira Mateus Roberto , Cavalcanti Erika Nayara Freire , Villegas-Cayllahua Erick Alonso , Fidelis Heloisa de Almeida , Giampietro-Ganeco Aline , Fávero Maísa Santos , Souza Pedro Alves de , Borba Hirasilva
Published: June 2021

This study aimed to evaluate the effects of aging on the quality of breast meat from broilers affected of wooden breast. Samples that were classified as normal (control), moderate (hardness verified only in one region of breast fillet), and severe (hardness verified in all the extension of breast fillet) were evaluated fresh and after three and seven days of aging. Normal samples and samples with a moderate degree of myopathy showed greater water-holding capacity, which may benefit the processing industry of poultry meat. During the aging process, increase was observed in total collagen concentration (from 0.41% in normal samples to 0.56% in samples with severe degree). Samples of chicken breast affected by moderate degree showed higher myofibril fragmentation index (MFI = 115) than normal chicken samples (95.65). Although chicken samples affected with severe degree of wooden breast myopathy are more tender than normal chicken breasts, they produce more exudate, which can be detrimental to the processing of poultry meat. The aging process may improve the reduction of cooking weight loss and protein loss in exudation of broilers’ breasts affected by wooden breast myopathy.
Assisted Reproductive Technology in Neotropical Deer: A Model Approach to Preserving Genetic Diversity
Authors: Rola Luciana Diniz , Buzanskas Marcos Eli , Melo Luciana Magalhães , Chaves Maiana Silva , Freitas Vicente José Figueirêdo , Duarte José Maurício Barbanti
Published: June 2021

One of the most significant challenges in deer is the ability to maintain genetic diversity, avoiding inbreeding and sustaining population health and reproduction. Although our general knowledge of reproductive physiology is improving, it appears that the application of assisted reproductive technology (ART) will more efficiently advance wildlife conservation efforts and preserve genetic diversity. The purpose of this review is to present the most important results obtained with the use of ART in Neotropical deer. Thus, the state-of-the-art for estrus synchronization, semen technology, artificial insemination, and in vivo embryo production will be presented. In vitro embryo production (IVP) is also a biotechnology that is taking initial steps in deer. In this aspect, the approach with the proteomics of ovarian follicular fluid is being used as a tool for a better understanding of oocyte maturation. Finally, cell banks and the use of interspecific somatic cell nuclear transfer (iSCNT) as well as the use of stem cells for gametes differentiation are promising techniques.
Beneficial Role of Antioxidant Secondary Metabolites from Medicinal Plants in Maintaining Oral Health
Authors: Kumar Manoj , Prakash Suraj , Radha  , Kumari Neeraj , Pundir Ashok , Punia Sneh , Saurabh Vivek , Choudhary Poonam , Changan Sushil , Dhumal Sangram , Pradhan Prakash Chandra , Alajil Omar , Singh Sudha , Sharma Neha , Ilakiya Tamilselvan , Singh Surinder , Mekhemar Mohamed
Published: June 2021

Plant-derived phytochemicals have been touted as viable substitutes in a variety of diseases. All over the world, dentists have turned to natural remedies for dental cure due to the negative possessions of certain antibacterial mediators used in dentistry. Antimicrobial and other drugs are currently in use, but they show some side effects. Since ancient times, antioxidant EOs have been used for different ailments and have grown in popularity over time. Several in vitro, in vivo, and clinical trials have shown the safety and effectiveness of antioxidant essential oils (EOs) in oral health obtained from medicinal plants. The current review of literature provides a summary of secondary metabolites, more specifically EOs from 20 most commonly used medicinal plants and their applications in maintaining oral health. Dental caries and periodontal diseases are the most common and preventable global infectious diseases, with diseases of the oral cavity being considered major diseases affecting a person’s health. Several clinical studies have shown a connection between oral diseases and oral microbiota. This review discusses the role of antioxidant secondary metabolites in inhibiting the growth of oral pathogens and reducing the formation of dental plaque, and as well as reducing the symptoms of oral diseases. This review article contributes a basic outline of essential oils and their healing actions.
Applications of Machine Learning and High-Performance Computing in the Era of COVID-19
Authors: Majeed Abdul , Lee Sungchang
Published: June 2021

During the ongoing pandemic of the novel coronavirus disease 2019 (COVID-19), latest technologies such as artificial intelligence (AI), blockchain, learning paradigms (machine, deep, smart, few short, extreme learning, etc.), high-performance computing (HPC), Internet of Medical Things (IoMT), and Industry 4.0 have played a vital role. These technologies helped to contain the disease’s spread by predicting contaminated people/places, as well as forecasting future trends. In this article, we provide insights into the applications of machine learning (ML) and high-performance computing (HPC) in the era of COVID-19. We discuss the person-specific data that are being collected to lower the COVID-19 spread and highlight the remarkable opportunities it provides for knowledge extraction leveraging low-cost ML and HPC techniques. We demonstrate the role of ML and HPC in the context of the COVID-19 era with the successful implementation or proposition in three contexts: (i) ML and HPC use in the data life cycle, (ii) ML and HPC use in analytics on COVID-19 data, and (iii) the general-purpose applications of both techniques in COVID-19’s arena. In addition, we discuss the privacy and security issues and architecture of the prototype system to demonstrate the proposed research. Finally, we discuss the challenges of the available data and highlight the issues that hinder the applicability of ML and HPC solutions on it.
Seasonality and Dynamics of Atmospheric Teleconnection from the Tropical Indian Ocean and the Western Pacific to West Antarctica
Authors: Lee Hyun-Ju , Jin Emilia-Kyung
Published: June 2021

The global impact of the tropical Indian Ocean and the Western Pacific (IOWP) is expected to increase in the future because this area has been continuously warming due to global warming; however, the impact of the IOWP forcing on West Antarctica has not been clearly revealed. Recently, ice loss in West Antarctica has been accelerated due to the basal melting of ice shelves. This study examines the characteristics and formation mechanisms of the teleconnection between the IOWP and West Antarctica for each season using the Rossby wave theory. To explicitly understand the role of the background flow in the teleconnection process, we conduct linear baroclinic model (LBM) simulations in which the background flow is initialized differently depending on the season. During JJA/SON, the barotropic Rossby wave generated by the IOWP forcing propagates into the Southern Hemisphere through the climatological northerly wind and arrives in West Antarctica; meanwhile, during DJF/MAM, the wave can hardly penetrate the tropical region. This indicates that during the Austral winter and spring, the IOWP forcing and IOWP-region variabilities such as the Indian Ocean Dipole (IOD) and Indian Ocean Basin (IOB) modes should paid more attention to in order to investigate the ice change in West Antarctica.
Anabatic Flow along a Uniformly Heated Slope Studied through Large-Eddy Simulation
Authors: Cintolesi Carlo , Di Santo Dario , Barbano Francesco , Di Sabatino Silvana
Published: June 2021

Anabatic flows are common phenomena in the presence of sloping terrains, which significantly affect the dynamics and the exchange of mass and momentum in the low-atmosphere. Despite this, very few studies in the literature have tackled this topic. The present contribution addresses this gap by utilising high-resolved large-eddy simulations for investigating an anabatic flow in a simplified configuration, commonly used in laboratory experiments. The purpose is to analyse the complex thermo-fluid dynamics and the turbulent structures arising from the anabatic flow near the slope. In such a flow, three main dynamic layers are identified and reported: the conductive layer close to the surface, the convective layer where the most energetic motion develops, and the outer region, which is almost unperturbed. The analysis of instantaneous fields reveals the presence of thermal plumes, which are stable turbulent structures enhancing vertical transport and mixing of momentum and temperature. Such structures are generated by thermal instabilities in the conductive layer that trigger the rise of the plumes above them. Their evolution along the slope is described, identifying three regions responsible for the plumes generation, stabilisation, and merging. To the best of the authors’ knowledge, this is the first numerical experiment describing the along-slope behaviour of the thermal plumes in the convective layer.
Source Analysis and Human Health Risk Assessment Based on Entropy Weight Method Modification of PM2.5 Heavy Metal in an Industrial Area in the Northeast of China
Authors: Sun Siyu , Zheng Na , Wang Sujing , Li Yunyang , Hou Shengnan , Song Xue , Du Shanshan , An Qirui , Li Pengyang , Li Xiaoqian , Hua Xiuyi , Dong Deming
Published: June 2021

In this study, PM2.5 was analyzed for heavy metals at two sites in industrial northeast China to determine their sources and human health risks during heating and non-heating periods. A positive matrix factorization (PMF) model determined sources, and US Environmental Protection Agency (USEPA) and entropy weight methods were used to assess human health risk. PM2.5 heavy metal concentrations were higher in the heating period than in the non-heating period. In the heating period, coal combustion (59.64%) was the primary heavy metal source at Huagong Hospitals, and the contribution rates of industrial emissions and traffic emissions were 21.06% and 19.30%, respectively. Industrial emissions (42.14%) were the primary source at Xinqu Park, and the contribution rates of coal combustion and traffic emissions were 34.03% and 23.83%, respectively. During the non-heating period, coal combustion (45.29%) and industrial emissions 45.29% and 44.59%, respectively, were the primary sources at Huagong Hospital, and the traffic emissions were 10.12%. Industrial emissions (43.64%) were the primary sources at Xinqu Park, where the coal combustion and traffic emissions were 25.35% and 31.00%, respectively. In the heating period, PM2.5 heavy metals at Xinqu Park had noncarcinogenic and carcinogenic risks, and the hazard index of children (5.74) was higher than that of adult males (5.28) and females (4.49). However, adult males and females had the highest lifetime carcinogenic risk (1.38 × 10−3 and 1.17 × 10−3) than children (3.00 × 10−4). The traditional USEPA and entropy weight methods both produced reasonable results. However, when there is a difference between the two methods, the entropy weight method is recommended to assess noncarcinogenic health risks.
Impacts of Non-Local versus Local Moisture Sources on a Heavy (and Deadly) Rain Event in Israel
Authors: Lynn Barry , Yair Yoav , Levi Yoav , Ziv Shlomi Ziskin , Reuveni Yuval , Khain Alexander
Published: June 2021

Motivated by poor forecasting of a deadly convective event within the Levant, the factor separation technique was used to investigate the impact of non-local versus local moisture sources on simulated precipitation and lightning rates in central and southern Israel on 25 and 26 April 2018. Both days saw unusually heavy rains, and it was hypothesized that antecedent precipitation on 25 April contributed to the development of deadly flooding late morning on the 26th, as well as strong lightning and heavy rains later the same day. Antecedent precipitation led to an increase in the precipitable water content and an overall increase in instability as measured by the Convective Available Potential Energy (CAPE). The deadly flood occurred in the area of the Tzafit river gorge (hereafter, Tzafit river), about 25 km southeast of the city of Dimona, a semi-arid region in the northeastern Negev desert. The heavy rains and strong lightning occurred throughout the Levant with local peaks in the vicinity of Jerusalem. Factor separation conducted in model simulations showed that local ground moisture sources had a large impact on the CAPE and subsequent precipitation and lightning rates in the area of Jerusalem, while non-local moisture sources enabled weak convection to occur over broad areas, with particularly strong convection in the area of the Tzafit river. The coupled impact of both moisture sources also led to localized enhanced areas of convective activity. The results suggest that forecast models for the Levant should endeavor to incorporate an accurate depiction of soil moisture to predict convective rain, especially during the typically drier spring-time season.
Near-Infrared-Triggered Upconverting Nanoparticles for Biomedicine Applications
Authors: Mahata Manoj Kumar , De Ranjit , Lee Kang Taek
Published: June 2021

Due to the unique properties of lanthanide-doped upconverting nanoparticles (UCNP) under near-infrared (NIR) light, the last decade has shown a sharp progress in their biomedicine applications. Advances in the techniques for polymer, dye, and bio-molecule conjugation on the surface of the nanoparticles has further expanded their dynamic opportunities for optogenetics, oncotherapy and bioimaging. In this account, considering the primary benefits such as the absence of photobleaching, photoblinking, and autofluorescence of UCNPs not only facilitate the construction of accurate, sensitive and multifunctional nanoprobes, but also improve therapeutic and diagnostic results. We introduce, with the basic knowledge of upconversion, unique properties of UCNPs and the mechanisms involved in photon upconversion and discuss how UCNPs can be implemented in biological practices. In this focused review, we categorize the applications of UCNP-based various strategies into the following domains: neuromodulation, immunotherapy, drug delivery, photodynamic and photothermal therapy, bioimaging and biosensing. Herein, we also discuss the current emerging bioapplications with cutting edge nano-/biointerfacing of UCNPs. Finally, this review provides concluding remarks on future opportunities and challenges on clinical translation of UCNPs-based nanotechnology research.