bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-Wide Association Studies Reveal Candidate Genes Related to Stem Diameter in Cucumber (Cucumis sativus L.)
Authors: Yang Yingying , Dong Shaoyun , Miao Han , Liu Xiaoping , Dai Zhuonan , Li Xiangsheng , Gu Xingfang , Zhang Shengping
Published: June 2022

The stem diameter, an important agronomic trait, affects cucumber growth and yield. However, no genes responsible for cucumber stem diameter have been identified yet. In this study, the stem diameter of 88 cucumber core germplasms were measured in spring 2020, autumn 2020 and autumn 2021, and a genome-wide association study (GWAS) was carried out based on the gene sequence and stem diameter of core germplasms. A total of eight loci (gSD1.1, gSD2.1, gSD3.1, gSD3.2, gSD4.1, gSD5.1, gSD5.2, and gSD6.1) significantly associated with cucumber stem diameter were detected. Of these, five loci (gSD1.1, gSD2.1, gSD3.1, gSD5.2, and gSD6.1) were repeatedly detected in two or more seasons and were considered as robust and reliable loci. Based on the linkage disequilibrium sequences of the associated SNP loci, 37 genes were selected. By further investigating the five loci via analyzing Arabidopsis homologous genes and gene haplotypes, five genes (CsaV3_1G028310, CsaV3_2G006960, CsaV3_3G009560, CsaV3_5G031320, and CsaV3_6G031260) showed variations in amino acid sequence between thick stem lines and thin stem lines. Expression pattern analyses of these genes also showed a significant difference between thick stem and thin stem lines. This study laid the foundation for gene cloning and molecular mechanism study of cucumber stem development.
Comparative Transcriptome Analysis of Organ-Specific Adaptive Responses to Hypoxia Provides Insights to Human Diseases
Authors: Hung Kuo-Sheng , Chen Shiow-Yi , Hsu Pang-Hung , Lin Bo-An , Hu Chin-Hua , Yang Cing-Han , Pai Tun-Wen , Tzou Wen-Shyong , Chung Hsin-Yu
Published: June 2022

The common carp is a hypoxia-tolerant fish, and the understanding of its ability to live in low-oxygen environments has been applied to human health issues such as cancer and neuron degeneration. Here, we investigated differential gene expression changes during hypoxia in five common carp organs including the brain, the gill, the head kidney, the liver, and the intestine. Based on RNA sequencing, gene expression changes under hypoxic conditions were detected in over 1800 genes in common carp. The analysis of these genes further revealed that all five organs had high expression-specific properties. According to the results of the GO and KEGG, the pathways involved in the adaptation to hypoxia provided information on responses specific to each organ in low oxygen, such as glucose metabolism and energy usage, cholesterol synthesis, cell cycle, circadian rhythm, and dopamine activation. DisGeNET analysis showed that some human diseases such as cancer, diabetes, epilepsy, metabolism diseases, and social ability disorders were related to hypoxia-regulated genes. Our results suggested that common carp undergo various gene regulations in different organs under hypoxic conditions, and integrative bioinformatics may provide some potential targets for advancing disease research.
Pride and Prejudice in Brazil’s Popular Culture: A Photonovel and a Soap Opera
Authors: Biajoli Maria Clara Pivato
Published: June 2022

Soap operas are an integral part of Brazilian popular culture and the daily lives of Brazil’s people. In 2018, the biggest TV channel in the country, Globo, broadcast a six-month-long soap opera called ‘Pride and Passion’, centered on the story of the Benedito family and their five unmarried daughters, who live in the small village of ‘Vale do Café’ (‘Coffee Valley’) around the 1910s, surrounded by the rural aristocracy and its coffee plantations. The obvious inspiration is Austen’s Pride and Prejudice, and its choice is an indication of Austen’s growing popularity outside English-speaking countries. This adaptation, which incorporates characters from her other novels as well, is the quintessential amalgamation of cultures and media, combining a canonical author of the English language with a Brazilian TV genre commonly seen as ‘lowbrow’. It was not, however, Austen’s first incursion in Brazil’s popular culture. During the 1960s and 1970s, photonovels were an extremely popular genre there, usually translated into Portuguese from Italian productions, as was the case of the 1965 Pride and Prejudice photonovel, sold as a literary supplement to a widely circulated women’s magazine. This essay analyses both cases of different, although connected, adaptations of Austen, arguing that Austen’s presence in Brazil was always mediated by the expectations and appropriation of new media, while showing that the dialogue with popular culture can only enhance our understanding of the ‘global Austen’ phenomenon and her appeal across time and cultures.
Association between Fear of Falling and Seven Performance-Based Physical Function Measures in Older Adults: A Cross-Sectional Study
Authors: Huang Wen-Ni Wennie , Mao Hui-Fen , Lee Hsin-Min , Chi Wen-Chou
Published: June 2022

Fear of falling (FOF), a common phenomenon among older adults, may result in adverse health consequences. The strength of the association between FOF and physical function among older adults has not been well compared in previous studies. Therefore, a cross-sectional study was performed on 105 older adults to determine and compare the strength of the association between FOF and seven common physical function measures. After controlling for age, logistic regression models were fitted for each physical function measure. According to odds ratios, the Berg Balance Scale (BBS), Short Physical Performance Battery, gait speed, and Timed Up & Go Test were associated with the identification of FOF. Based on a c-statistic value of 0.76, the BBS, a common and quick assessment of functional balance tasks, was found to be able to distinguish between fearful and non-fearful older adults. Interventions targeted to improve lower-extremity physical functions, especially functional balance ability, may help prevent or delay the adverse consequences of FOF.
Knowledge and Use of Cervical Cancer Prevention Services among Social Work and Nursing University Students
Authors: Moudatsou Maria , Vouyiouka Panayiota , Karagianni-Hatziskou Eleni , Rovithis Michael , Stavropoulou Areti , Koukouli Sofia
Published: June 2022

The present study examines: (a) the knowledge of healthcare students on cervical cancer (CC) issues and the use of related preventive services, as well as their association with the field of study and other sociodemographic characteristics; (b) the possible effect of social capital and its parameters. A cross-sectional study was conducted, using a convenience non-probability sampling technique. The final sample consisted of forty-nine social work and fifty-one nursing students. The two groups were similar regarding their sociodemographic characteristics and the knowledge and use of gynecological preventive services. However, the nursing students undertook a PAP smear check-up to a lesser extent (48.6%) compared to social work students (51.4%) (p = 0.026). The social capital scores were high for both groups, but social work students were significantly more ‘Tolerant to diversity’. For the total sample, only the ‘Family and friends connections’ subscale correlated with knowledge about the existing gynecological preventive services. Among the main reasons explaining university students’ avoidance of preventive testing were the feelings of fear and embarrassment associated with the PAP smear test. Given the significance of the future professional roles of healthcare students as information sources and leaders in women’s CC preventive behavior, understanding the individual factors contributing to their own adherence is essential. It is equally important to increase their scientific knowledge through the improvement of academic curricula regarding these issues.
Use of the Nursing Interventions Classification and Nurses’ Workloads: A Scoping Review
Authors: Rodríguez-Suárez Claudio-Alberto , Rodríguez-Álvaro Martín , García-Hernández Alfonso-Miguel , Fernández-Gutiérrez Domingo-Ángel , Martínez-Alberto Carlos-Enrique , Brito-Brito Pedro-Ruymán
Published: June 2022

Background: The Nursing Interventions Classification allows the systematic organisation of care treatments performed by nurses, and an estimation of the time taken to carry out the intervention is included in its characteristics. The aim of this study is to explore the evidence related to the use of the Nursing Interventions Classification in identifying and measure nurses’ workloads. Methods: A scoping review was conducted through a search of the databases Ovid Medline, PubMed, Web of Science, CINAHL, Scopus, LILACS and Cuiden. The DeCS/MeSH descriptors were: “Standardized Nursing terminology” and “Workload”. The search was limited to articles in Spanish, English and Portuguese. No limits were established regarding year of publication or type of study. Results: Few reports were identified (n = 8) and these had methodological designs that contributed low levels of evidence. Research was focused on identifying specific interventions, types of activities, the prevalence of interventions and the time required to perform them. Conclusions: The evidence found on determination of nurses’ workloads using the Nursing Interventions Classification was inconclusive. It is essential to increase the number of reports, as well as the settings and clinical context in which the Nursing Interventions Classification is used, with greater quality and methodological rigour.
Rhizosphere Acidification as the Main Trait Characterizing the Differential In Vitro Tolerance to Iron Chlorosis in Interspecific Pyrus Hybrids
Authors: Mora-Córdova Claudia Paola , Tolrà Roser , Padilla Rosa , Poschenrieder Charlotte , Simard Marie-Helene , Asín Luis , Vilardell Pere , Bonany Joan , Claveria Elisabet , Dolcet-Sanjuan Ramon
Published: June 2022

Physiological responses of different interspecific Pyrus hybrids and an open pollinated Pyrus communis ‘Williams’ (Pcw) grown under in vitro culture conditions simulating lime induced chlorosis were studied. The hybrids were derived from crosses between the ‘Pyriam’ pear rootstock and four Pyrus species of the Mediterranean region, namely P. amygdaliformis Vill. (Pa), P. amygdaliformis persica Bornme. (Pap), P. communis cordata (Desv.) Hook. (Pcc), and P. elaeagrifolia Pall (Pe), all known for their higher field tolerance to iron-chlorosis than P. communis. Twenty hybrids and one open pollinated Pcw were micropropagated, and plantlets were in vitro characterized for their physiological responses to iron-deficiency conditions. Rooted plantlets were transferred to a culture medium with 2 µM Fe3+ DTPA and 10 or 20 mM NaHCO3. These physiological responses were scored at 1, 3, 7, and 28 days from the start of the in vitro assay. Leaf total chlorophyll content, the capacity of roots to acidify the medium, reduced iron, and exudates of phenolic acids and organic acids were analyzed in each media and time sample. Leaf chlorophyll levels for the clones derived from Pcc were the highest, especially under the highest bicarbonate concentration, followed by those derived from Pap and Pa. The higher chlorophyll content of Pcc clones were related with their higher capacity to acidify the media but not with their iron reduction capacity at the root level. On the other hand, hybrid clones derived from Pe showed a higher Fe3+ reduction ability than clones from all the other species during the whole assay but only when the bicarbonate concentration was lower. The exudation of phenolic acids by the roots was higher in Pcw than in the other species, and this response might explain why the total chlorophyll levels in Pcw clones are similar to those of Pe and Pa ones. These results with Pyrus spp. bring more evidence in support of the idea that iron reduction capacity at the root level is not directly related with a higher tolerance to iron deficiency caused by the high pH of calcareous soils. Instead, the ability to acidify the rhizosphere is the trait of choice for the selection of the pear hybrid clones better adapted to lime induced chlorosis. In addition, the in vitro assay to select the Pyrus clones for tolerance to iron chlorosis could be shortened to one week of culture in 10 mM NaHCO3, measuring the leaf chlorophyll level, acidification of the culture medium, and exudation of phenolic acids as the physiological responses to predict tolerance to lime-induced chlorosis.
Trunk Injection as a Tool to Deliver Plant Protection Materials—An Overview of Basic Principles and Practical Considerations
Authors: Archer Leigh , Crane Jonathan H. , Albrecht Ute
Published: June 2022

Trunk injection is a targeted delivery of pesticides, insecticides, nutrients, or other plant protection materials into the stem or trunk of woody plants as an alternative to spraying or soil drenching. Trunk injection has historically been used for disease and pest management of high-value forest tree species or ornamental plants when aerial applications are problematic due to spatial problems and health-related concerns. An interest in using the injection technique for protection of agricultural crops in commercial production systems has emerged more recently, where foliar applications and soil drenches have proven ineffective or pose environmental hazards. This review provides an overview of the basic principles of trunk injection and the plant physiological implications, its current use in commercial agriculture and other plant systems, and associated risks.
Disinfection Efficacy of Tobamovirus-Contaminated Soil in Greenhouse-Grown Crops
Authors: Dombrovsky Aviv , Mor Netta , Gantz Shelly , Lachman Oded , Smith Elisheva
Published: June 2022

The tobamoviruses tomato brown rugose fruit virus (ToBRFV) and cucumber green mottle mosaic virus (CGMMV) have caused severe crop damages worldwide. Soil-mediated dispersion of the mechanically transmitted tobamoviruses constitute a major hindrance toward mitigating disease spread in crops carefully planted under sanitized conditions. Tobamoviruses are viable for months in soil and plant debris and for more than a year adhere to clay. However, a low percentage of infectious foci occur in soil following a tobamovirus-infected growing cycle, rendering disinfection studies of several contaminated plots inconclusive for large-scale crop productions. We have therefore formulated a rigorous platform for studying disinfectant efficacy in greenhouses by pouring a virus inoculum to planting pits prior to disinfectant treatment and by truncating seedling roots before planting, which was otherwise conducted under sanitized conditions. We have found that chlorine-based Taharan was significantly efficient in preventing disease spread of ToBRFV and CGMMV in tomato and cucumber plants, respectively. KlorBack was often as good as Taharan. In addition, a formulation of chlorinated tri-sodium phosphate used at a nonphytotoxic 3% concentration showed disinfection efficiency similar to Taharan effect on ToBRFV infection only. Our study provided a small-scale platform for disinfectant efficacy evaluation necessary for application in tobamovirus-contaminated soil, which commonly occurs in commercial tomato and cucumber greenhouses.
Multi-Variable SWAT Model Calibration Using Satellite-Based Evapotranspiration Data and Streamflow
Authors: Koltsida Evgenia , Kallioras Andreas
Published: June 2022

In this study, monthly streamflow and satellite-based actual evapotranspiration data (AET) were used to evaluate the Soil and Water Assessment Tool (SWAT) model for the calibration of an experimental sub-basin with mixed land-use characteristics in Athens, Greece. Three calibration scenarios were performed using streamflow (i.e., single variable), AET (i.e., single variable), and streamflow–AET data together (i.e., multi-variable) to provide insights into how different calibration scenarios affect the hydrological processes of a catchment with complex land use characteristics. The actual evapotranspiration data were obtained from the Moderate Resolution Imaging Spectroradiometer (MODIS). The calibration was achieved with the use of the SUFI-2 algorithm in the SWAT-CUP program. The results suggested that the single variable calibrations showed moderately better performance than the multi-variable calibration. However, the multi-variable calibration scenario displayed acceptable outcomes for both streamflow and actual evapotranspiration and indicated reasonably good streamflow estimations (NSE = 0.70; R2 = 0.86; PBIAS = 6.1%). The model under-predicted AET in all calibration scenarios during the dry season compared to MODIS satellite-based AET. Overall, this study demonstrated that satellite-based AET data, together with streamflow data, can enhance model performance and be a good choice for watersheds lacking sufficient spatial data and observations.
Parenting Practice Profiling and Its Associated Factors among Secondary Vocational School Students in China
Authors: Zheng Yujia , Fang Yuhang , Jin Yan , Zuo Xiayun , Lian Qiguo , Lou Chaohua , Yu Chunyan , Tu Xiaowen , Li Lihe , Hong Ping
Published: June 2022

Background: Parenting styles have a tremendous influence on a child’s development and behavior. Studies on parenting styles using latent profile analysis have been increasing in recent years. However, there are few such studies conducted in China, especially concerning joint parenting styles (that simultaneously characterize maternal and paternal practices), which are held over the age group of secondary vocational school students. This study aimed to identify the profiles of parenting styles and their associated factors among the parents of secondary vocational school students in China, based on natural samples and not a predetermined model. Method: Data were drawn from a cross-sectional study conducted among 3180 students from six secondary vocational schools in Shanghai Municipality and Shaanxi Province. A total of 2392 students who have lived with their parents for most of their lifetime were included in the study. Latent profile analysis was used to identify the profiles of parenting styles of the respondent’s parents. Multinominal logistic regression models were used to examine the association between parenting style and demographic characteristics and family background and adolescent outcomes. Results: We identified five latent profiles: “free-range parenting” (27.05%), “behavioral monitoring parenting” (33.65%), “authoritative parenting” (11.75%), “psychological control parenting” (14.38%) and “tiger parenting” (13.17%). The associations between these profiles and adolescent outcomes indicated that these profiles were rational. Participants’ gender, grade, residential area, family economic level, parental marital relationship, and parental educational level were predictive factors for parenting styles. Conclusions: The parenting styles held over secondary vocational school students were somewhat different from Baumrind’s parenting style model. A considerable number of students received a parenting style that might predispose them to behavioral and mental health outcomes and merit a tailored intervention using the predictive factors of parenting styles.
Tobacco Use and Exposure to Environmental Tobacco Smoke amongst Pregnant Women in the United Arab Emirates: The Mutaba’ah Study
Authors: Taha Mohammed Nagdi , Al-Ghumgham Zaki , Ali Nasloon , Al-Rifai Rami H. , Elbarazi Iffat , Al-Maskari Fatima , El-Shahawy Omar , Ahmed Luai A. , Loney Tom
Published: June 2022

Self-reported tobacco use is high in the male adult Emirati population (males ~36% vs. females ~3%); however, there are minimal data on tobacco use or exposure to environmental tobacco smoke (ETS) during pregnancy in the United Arab Emirates (UAE). This study investigated the prevalence of, and factors associated with, tobacco use and exposure to environmental tobacco smoke (ETS) amongst pregnant women in the UAE. Baseline cross-sectional data were analysed from the Mutaba’ah Study. Expectant mothers completed a self-administered questionnaire collecting sociodemographic information, maternal tobacco use, and ETS exposure during antenatal visits at three hospitals in Al Ain (UAE; May 2017–February 2021). Amongst 8586 women included in the study, self-reported tobacco use during pregnancy was low (0.7%), paternal tobacco use was high (37.9%), and a third (34.8%) of expectant mothers were exposed to ETS (28.0% at home only). Pregnant women who were employed (adjusted odds ratio (aOR): 1.35, 95% confidence interval (CI): 1.19–1.52), with childbirth anxiety (aOR 1.21, 95% CI 1.08–1.36), and with an increased number of adults living in the same household (aOR 1.02 95% CI 1.01–1.03) were independently more likely to be exposed to ETS. Pregnant women with higher education levels (aOR 0.84, 95% CI 0.75–0.94) and higher gravidity (aOR 0.95, 95% CI 0.92–0.99) were less likely to be exposed to ETS. Public health efforts targeting smoking cessation amongst husbands and promoting smoke-free homes are warranted to help reduce prenatal ETS exposure in the UAE.
Perceived Stigma of Patients Undergoing Treatment with Cannabis-Based Medicinal Products
Authors: Troup Lucy J. , Erridge Simon , Ciesluk Beata , Sodergren Mikael H.
Published: June 2022

Cannabis-based medicinal products (CBMPs) are prescribed with increasing frequency. This study aimed to investigate the perceived stigma attached to patients prescribed CBMPs in the UK to establish its prevalence. A qualitative survey was developed by an expert multidisciplinary group and data were collected via Qualtrics. In total, 2319 patients on CBMP therapy were invited to take part in this study. 450 (19.4%) participants completed the questionnaire. In total, 81.3% (n = 366), 76.9% (n = 346), and 61.3% (n = 276) of participants reported feeling very comfortable or comfortable telling friends, family, and medical professionals, respectively, about their treatment. Participants thought that friends (n = 372; 82.7%) and family (n = 339; 75.3%) were very approving or somewhat approving of their CBMP prescription. However, participants thought that only 37.8% (n = 170) of healthcare professionals and 32.9% (n = 148) of society in general were very approving or somewhat approving of their CBMP prescription. 57.1% (n = 257), 55.3% (n = 249), and 40.2% (n = 181) of participants were afraid of what the police or criminal justice system, other government agencies, and healthcare professionals might think about their treatment. This study highlights those patients treated with CBMPs experience a high prevalence of perceived stigma from many corners of society. Future work should be undertaken to explore strategies to reduce perceived stigma at an individual and community level to avoid discrimination of patients, likely increasing appropriate access.
The Mediating Role of Compassion between Social Job Resources, and Healthy Healthcare Professionals: A Cross-Sectional Study with Gender Perspective
Authors: San Román-Niaves Mabel , Coo Cristián , Llorens Susana , Salanova Marisa
Published: June 2022

The aim of this study is to examine the role of Compassion towards others as a mediator between Social Job Resources (social support climate, coordination, and positive leadership), Healthy Employees (psychological well-being such as resilience, engagement, and optimism) and Healthy Organisational Outcomes (in-role performance, extra-role performance and commitment) from a gender perspective in healthcare professionals. Through the multiple analyses of variance, structural equation models, and multiple-group analyses in a sample of 1420 healthcare professionals from different public and private hospitals in Spain, this study proved the existence of gender differences, with women perceiving higher levels of Compassion. Moreover, this study shows that Compassion partially mediates the relationship between Social Job Resources and Healthy Employees. In addition, Compassion partially mediates the relationship between Social Job Resources and Healthy Organisational Outcomes. Finally, Healthy Employees mediate the positive relationship between Social Job Resources and Healthy Organisational Outcomes. This is an innovative contribution to the limited research examining Compassion towards others as a personal resource that can have a positive impact in the workplace. The results also propose a way to develop and conduct interventions in order to increase Compassion towards others in the healthcare context.
Health Promotion Behaviors of Pregnant Couples in the COVID-19 Pandemic: Actor-Partner Interdependence Model
Authors: Kim Sun Hee , Kang Hee Sun
Published: June 2022

Pregnancy during the pandemic may be a stressful life event. This cross-sectional study aimed to identify the actor and partner effects of the fear of COVID-19, depression, posttraumatic growth, and marital adjustment on the health promotion behaviors of pregnant couples during the COVID-19 pandemic in Korea. Data were collected from 123 pregnant couples using a structured questionnaire. The data were analyzed using the Actor-Partner Interdependence Model (APIM). The actor effects of the fear of COVID-19, depression, and posttraumatic growth on the health promotion behaviors of pregnant women and their spouses were significant. Furthermore, both actor and partner effects of husbands’ marital adjustment on health promotion behaviors were significant. When carrying out nursing intervention for the health promotion of pregnant couples, programs aimed at reducing depression and improving posttraumatic growth in pregnant couples should be included. In addition, improving marital adjustment will positively affect the health promotion behaviors of pregnant couples. The findings highlight the important role of healthcare providers in assessing depressive symptoms and fear of COVID-19 in pregnant couples and providing support to promote health behaviors as part of prenatal care.
Aboriginal Population and Climate Change in Australia: Implications for Health and Adaptation Planning
Authors: Standen Jeffrey , Spencer Jessica , Lee Grace , Van Buskirk Joe , Matthews Veronica , Hanigan Ivan , Boylan Sinead , Jegasothy Edward , Breth-Petersen Matilde , Morgan Geoffrey
Published: June 2022

The health impacts of climate are widely recognised, and extensive modelling is available on predicted changes to climate globally. The impact of these changes may affect populations differently depending on a range of factors, including geography, socioeconomics and culture. This study reviewed current evidence on the health risks of climate change for Australian Aboriginal populations and linked Aboriginal demographic data to historical and projected climate data to describe the distribution of climate-related exposures in Aboriginal compared to non-Aboriginal populations in New South Wales (NSW), Australia. The study showed Aboriginal populations were disproportionately exposed to a range of climate extremes in heat, rainfall and drought, and this disproportionate exposure was predicted to increase with climate change over the coming decades. Aboriginal people currently experience higher rates of climate-sensitive health conditions and socioeconomic disadvantages, which will impact their capacity to adapt to climate change. Climate change may also adversely affect cultural practices. These factors will likely impact the health and well-being of Aboriginal people in NSW and inhibit measures to close the gap in health between Aboriginal and non-Aboriginal populations. Climate change, health and equity need to be key considerations in all policies at all levels of government. Effective Aboriginal community engagement is urgently needed to develop and implement climate adaptation responses to improve health and social service preparedness and secure environmental health infrastructure such as drinking water supplies and suitably managed social housing. Further Aboriginal-led research is required to identify the cultural impacts of climate change on health, including adaptive responses based on Aboriginal knowledges.
Intermittent Exercise at Lactate Threshold Induces Lower Acute Stress than Its Continuous Counterpart in Middle-to-Older Aged Men
Authors: Yamamoto Taiki , Tanoue Yukiya , Uehara Yoshinari , Higaki Yasuki , Tanaka Hiroaki , Narazaki Kenji
Published: June 2022

This study aimed to compare the degree of exhaustion and trophic effects between continuous exercise (CE) and intermittent exercise (IE) at lactate threshold (LT) intensity. Seven healthy men (age: 43–69 years) performed the following three experimental tests in a randomized crossover order: (1) control; (2) CE, performed as a 20-min of cycling at LT intensity; and (3) IE, performed as 20 sets of a one-min bout of cycling at LT intensity with a 30-s rest between every two sets. Heart rate (HR), blood lactate concentration (LA), rating of perceived exertion (RPE), catecholamines, cortisol, growth hormone, insulin-like growth factor (IGF)-1, and brain-derived neurotrophic factor (BDNF) were measured. The sampling timing in each test was as follows: 10 min before the onset of exercise, at the 25%, 50%, and 100% time points of exercise, and at 10 min after exercise. IE was found to be accompanied by a lower degree of exhaustion than CE in measures of HR, LA, RPE, catecholamines, and cortisol. In terms of trophic effects, both of IGF-1 and BDNF increased in CE, while a marginal increase of BDNF was observed in IE. The results indicated that IE induces lower stress than CE, but may not be effective for inducing trophic effects.
Applying Interleaving Strategy of Learning Materials and Perceptual Modality to Address Secondary Students’ Need to Restore Cognitive Capacity
Authors: Chen Wen , Chen Chuansheng , Li Baoping , Zhang Jiacai
Published: June 2022

Online courses are prevalent around the world, especially during the COVID-19 pandemic. Long hours of highly demanding online learning can lead to mental fatigue and cognitive depletion. According to Attention Restoration Theory, ‘being away’ or a mental shift could be an important strategy to allow a person to recover from the cognitive overload. The present study aimed to test the interleaving strategy as a mental shift method to help sustain students’ online learning attention and to improve learning outcomes. A total of 81 seventh-grade Chinese students were randomly assigned to four learning conditions: blocked (by subject matter) micro-lectures with auditory textual information (B-A condition), blocked (by subject matter) micro-lectures with visual textual information (B-V condition), interleaved (by subject matter) micro-lectures with auditory textual information (I-A condition), and interleaved micro-lectures by both perceptual modality and subject matter (I-all condition). We collected self-reported data on subjective cognitive load (SCL) and attention level, EEG data during the 40 min of online learning, and test results to assess learning outcomes. The results showed that the I-all condition showed the best overall outcomes (best performance, low SCL, and high attention). This study suggests that interleaving by both subject matter and perceptual modality should be preferred in scheduling and planning online classes.
Lifestyle, Physical Activity and Dietary Habits of Medical Students of Wroclaw Medical University during the COVID-19 Pandemic
Authors: Kosendiak Aureliusz Andrzej , Wysocki Michał Piotr , Krysiński Paweł Piotr
Published: June 2022

The new disease COVID-19, induced by SARS-CoV-2, causes acute respiratory infection. Many countries, including Poland, began to set a variety of different restrictions to reduce the spread of the virus. Most students had problems with online lessons. The study was conducted among second year medicine students of the Medical University of Wroclaw, and after the entire process of verification 200 respondents were accepted. The research consisted of completing the same anonymous online questionnaires twice in March and October 2020. This finally allowed for a critical assessment of the impact of the pandemic and its restrictions on the students’ daily lives. During the online classes, low levels of physical activity persisted (p = 0.718), whereas time spent sitting increased (p < 0.001). Despite positive changes in declared snacking (p = 0.061), we observed significant drops in the index of healthy diet (p = 0.001) and nutritional knowledge (p < 0.001) as well as an increase in the consumption of fast-food (p < 0.001) and energy drinks (p = 0.019). Reduced nutritional knowledge can cause a decrease in attention to healthy food preparation and much more frequent consumption of fast-food.
Bioleaching of Typical Electronic Waste—Printed Circuit Boards (WPCBs): A Short Review
Authors: Ji Xiaosheng , Yang Mindong , Wan Anping , Yu Shaoqi , Yao Zhitong
Published: June 2022

The rapid pace of innovations and the frequency of replacement of electrical and electronic equipment has made waste printed circuit boards (WPCB) one of the fastest growing waste streams. The frequency of replacement of equipment can be caused by a limited time of proper functioning and increasing malfunctions. Resource utilization of WPCBs have become some of the most profitable companies in the recycling industry. To facilitate WPCB recycling, several advanced technologies such as pyrometallurgy, hydrometallurgy and biometallurgy have been developed. Bioleaching uses naturally occurring microorganisms and their metabolic products to recover valuable metals, which is a promising technology due to its cost-effectiveness, environmental friendliness, and sustainability. However, there is sparse comprehensive research on WPCB bioleaching. Therefore, in this work, a short review was conducted from the perspective of potential microorganisms, bioleaching mechanisms and parameter optimization. Perspectives on future research directions are also discussed.
Mental and Physical Stress Responses to Personal Ultrafine Particle Exposure in Adolescents
Authors: Turner Ashley L. , Brokamp Cole , Wolfe Chris , Reponen Tiina , Brunst Kelly J. , Ryan Patrick H.
Published: June 2022

Incidence rates of mental health disorders among adolescents is increasing, indicating a strong need for effective prevention efforts at a population level. The etiology of mental health disorders includes genetic, social, and environmental factors. Ultrafine particles (UFPs; particles less than 0.1 μm in diameter) have been shown to exert neurotoxic effects on the brain; however, epidemiologic evidence on the relationship between UFPs and childhood mental health outcomes is unclear. The objective of this study was to determine if exposure to UFPs was associated with symptoms of mental health in adolescents. Adolescents completed personal UFP monitoring for one week as well as a series of validated Patient-Reported Outcomes Measurement Information System (PROMIS) assessments to measure five domains of mental and physical stress symptoms. Multivariable linear regression models were used to estimate the association between PROMIS domain T-scores and median weekly personal UFP exposure with the inclusion of interactions to explore sex differences. We observed that median weekly UFP exposure was significantly associated with physical stress symptoms (β: 5.92 per 10-fold increase in UFPs, 95% CI [0.72, 11.13]) but no other measured domains. Further, we did not find effect modification by sex on any of the PROMIS outcomes. The results of this study indicate UFPs are associated with physical symptoms of stress response among adolescents, potentially contributing to mental health disorders in this population.
Antioxidative Defense and Gut Microbial Changes under Pollution Stress in Carassius gibelio from Bucharest Lakes
Authors: Alistar Cristina F. , Nica Ionela C. , Nita-Lazar Mihai , Vasile Gabriela Geanina , Gheorghe Stefania , Croitoru Alexa-Maria , Dolete Georgiana , Mihaiescu Dan Eduard , Ficai Anton , Craciun Nicolai , Gradisteanu Pircalabioru Gratiela , Chifiriuc Mariana Carmen , Stan Miruna S. , Dinischiotu Anca
Published: June 2022

Fish are able to accumulate by ingestion various contaminants of aquatic environment, with negative consequences on their intestine, being continuously threatened worldwide by heavy metals, pesticides and antibiotics resulted from the human activities. Consequently, the health of other species can be affected by eating the contaminated fish meat. In this context, our study aimed to perform a comparison between the changes in intestine samples of Carassius gibelio individuals collected from different artificial lakes in Bucharest (Romania), used by people for leisure and fishing. The presence of various metals, pesticides and antibiotics in the gut of fish was assessed in order to correlate their accumulation with changes of antioxidative enzymes activities and microbiome. Our results showed that fish from Bucharest lakes designed for leisure (Chitila, Floreasca and Tei lakes) have an increased level of oxidative stress in intestine tissue, revealed by affected antioxidant enzymes activities and GSH levels, as well as the high degree of lipid peroxidation, compared to the fish from protected environment (Vacaresti Lake). Some heavy metals (Fe, Ni and Pb) and pesticides (aldrin and dieldrin) were in high amount in the gut of fish with modified antioxidative status. In conclusion, our study could improve the knowledge regarding the current state of urban aquatic pollution in order to impose several environmental health measures.
Recent Insights into Particulate Matter (PM2.5)-Mediated Toxicity in Humans: An Overview
Authors: Thangavel Prakash , Park Duckshin , Lee Young-Chul
Published: June 2022

Several epidemiologic and toxicological studies have commonly viewed ambient fine particulate matter (PM2.5), defined as particles having an aerodynamic diameter of less than 2.5 µm, as a significant potential danger to human health. PM2.5 is mostly absorbed through the respiratory system, where it can infiltrate the lung alveoli and reach the bloodstream. In the respiratory system, reactive oxygen or nitrogen species (ROS, RNS) and oxidative stress stimulate the generation of mediators of pulmonary inflammation and begin or promote numerous illnesses. According to the most recent data, fine particulate matter, or PM2.5, is responsible for nearly 4 million deaths globally from cardiopulmonary illnesses such as heart disease, respiratory infections, chronic lung disease, cancers, preterm births, and other illnesses. There has been increased worry in recent years about the negative impacts of this worldwide danger. The causal associations between PM2.5 and human health, the toxic effects and potential mechanisms of PM2.5, and molecular pathways have been described in this review.
Promoting Cancer Health Equity: A Qualitative Study of Mentee and Mentor Perspectives of a Training Program for Underrepresented Scholars in Cancer Health Disparities
Authors: Rogova Anastasia , Martinez Leal Isabel , Britton Maggie , Chang Shine , Escoto Kamisha H. , Solari Williams Kayce D. , Roberson Crystal , McNeill Lorna H. , Reitzel Lorraine R.
Published: June 2022

Racial and ethnic minorities, and women, experience stark disparities in cancer risk behaviors and mortality rates, yet often remain underrepresented in scientific research positions. We conducted an exploratory, qualitative study to examine the value of mentored research experience as part of an NCI-funded research training program designed to increase the representation of minority and women scientists in cancer disparities research. Using individual interviews, we explored 16 mentees’ and 7 mentors’ program experiences and perspectives to identify the most effective strategies to build strong mentoring relationships that could ultimately contribute to increased representation in health disparities research. Two expert analysts employed thematic analysis and constant comparison to code, categorize, and summarize the data into themes. Mentees and mentors shared five themes identifying contributions to program success: conditions for building successful mentoring relationships; role of mentor/mentee similarities or differences and their impact on effective collaboration; program elements that fostered developing knowledge, skills, and confidence; program supportive opportunities; and challenges and benefits of in-person vs. virtual program delivery during the COVID-19 pandemic. These findings contribute to improving the quality of training programs for historically excluded trainees to advance their cancer disparities research careers and offer a successful model that can guide similar programs.
Distinguishing the Effect of Time Spent at Home during COVID-19 Pandemic on the Mental Health of Urban and Suburban College Students Using Cell Phone Geolocation
Authors: Ayranci Pelin , Bandera Cesar , Phan NhatHai , Jin Ruoming , Li Dong , Kenne Deric
Published: June 2022

The aim of this study was to assess the correlation of depression and anxiety with time spent at home among students at two universities—one urban and the other suburban—during the COVID-19 pandemic. Methods: Geolocation data from the smartphones of 124 participants were collected between February 2021 and May 2021. The level of depression was estimated by the PHQ-9 and PHQ-2 screening tools, and anxiety scores were estimated by the GAD-2 and GAD-7 screening tools. Results: 51% of participants in the PHQ-9 surveys indicated mild to severe depression. Participants spent on average 75% of their time at home during COVID. Time spent at home had a positive correlation with the mental health of urban students but a negative correlation with suburban students. The relation between the time at home with mental health was stronger among female participants than among male participants. Correlations between female depression, anxiety, and time at home were significant. Conclusions: Lockdown and distance learning contributed to the high levels of depression in university students. This research highlights the importance of time spent at home for mental health being during the pandemic and the importance of distinguishing between urban and suburban settings when formulating public health recommendations. Quality of time spent at home versus time spent outside differentiated the mental well-being of students located in different environments. Staying at home may be recommended for students without access to safe outdoor places as it is associated with lower levels of depression.
The Effectiveness of Behavioral Interventions in Adults with Post-Traumatic Stress Disorder during Clinical Rehabilitation: A Rapid Review
Authors: Gimigliano Francesca , Young Vanessa M. , Arienti Chiara , Bargeri Silvia , Castellini Greta , Gianola Silvia , Lazzarini Stefano G. , Moretti Antimo , Heinemann Allen W. , Negrini Stefano
Published: June 2022

Background: This review examined the effectiveness of behavioral interventions for adults with post-traumatic stress disorder (PTSD) triggered by physical injury or medical trauma. It discusses implications in support of rehabilitation management for COVID-19 survivors diagnosed with PTSD. Methods: This study adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines and the Interim Guidance from the Cochrane Rapid Reviews Methods Group. The authors searched for randomized control trials in PubMed, Embase, and CENTRAL databases up to 31 March 2021. Results: Five studies (n = 459) met the inclusion criteria. Each study measured a different comparison of interventions. The certainty of the evidence was judged to be very low for all outcomes. Post-traumatic stress disorder symptom reduction was found to be in favor of trauma-focused cognitive-behavioral therapy, cognitive therapy, and cognitive-behavioral therapy. Cognitive function improvements were observed in favor of the cognitive processing therapy control intervention. Conclusions: Overall, there is uncertainty about whether behavioral interventions are effective in reducing PTSD symptoms and improving functioning and quality of life when the disorder is triggered by a physical or medical trauma rather than a psychological trauma. Further research should investigate their efficacy in the context of rehabilitation management and gather evidence on this population.
Derangements and Reversibility of Energy Metabolism in Failing Hearts Resulting from Volume Overload: Transcriptomics and Metabolomics Analyses
Authors: Tung Ying-Chang , Cheng Mei-Ling , Wu Lung-Sheng , Tang Hsiang-Yu , Huang Cheng-Yu , Chang Gwo-Jyh , Chang Chi-Jen
Published: June 2022

Derangements in cardiac energy metabolism have been shown to contribute to the development of heart failure (HF). This study combined transcriptomics and metabolomics analyses to characterize the changes and reversibility of cardiac energetics in a rat model of cardiac volume overload (VO) with the creation and subsequent closure of aortocaval fistula. Male Sprague–Dawley rats subjected to an aortocaval fistula surgery for 8 and 16 weeks exhibited characteristics of compensated hypertrophy (CH) and HF, respectively, in echocardiographic and hemodynamic studies. Glycolysis was downregulated and directed to the hexosamine biosynthetic pathway (HBP) and O-linked-N-acetylglucosaminylation in the CH phase and was further suppressed during progression to HF. Derangements in fatty acid oxidation were not prominent until the development of HF, as indicated by the accumulation of acylcarnitines. The gene expression and intermediates of the tricarboxylic acid cycle were not significantly altered in this model. Correction of VO largely reversed the differential expression of genes involved in glycolysis, HBP, and fatty acid oxidation in CH but not in HF. Delayed correction of VO in HF resulted in incomplete recovery of defective glycolysis and fatty acid oxidation. These findings may provide insight into the development of innovative strategies to prevent or reverse metabolic derangements in VO-induced HF.
Silencing the Tlr4 Gene Alleviates Methamphetamine-Induced Hepatotoxicity by Inhibiting Lipopolysaccharide-Mediated Inflammation in Mice
Authors: Wang Li-Bin , Chen Li-Jian , Wang Qi , Xie Xiao-Li
Published: June 2022

Methamphetamine (METH) is a stimulant drug. METH abuse induces hepatotoxicity, although the mechanisms are not well understood. METH-induced hepatotoxicity was regulated by TLR4-mediated inflammation in BALB/c mice in our previous study. To further investigate the underlying mechanisms, the wild-type (C57BL/6) and Tlr4−/− mice were treated with METH. Transcriptomics of the mouse liver was performed via RNA-sequencing. Histopathological changes, serum levels of metabolic enzymes and lipopolysaccharide (LPS), and expression of TLR4-mediated proinflammatory cytokines were assessed. Compared to the control, METH treatment induced obvious histopathological changes and significantly increased the levels of metabolic enzymes in wild-type mice. Furthermore, inflammatory pathways were enriched in the liver of METH-treated mice, as demonstrated by expression analysis of RNA-sequencing data. Consistently, the expression of TLR4 pathway members was significantly increased by METH treatment. In addition, increased serum LPS levels in METH-treated mice indicated overproduction of LPS and gut microbiota dysbiosis. However, antibiotic pretreatment or silencing Tlr4 significantly decreased METH-induced hepatic injury, serum LPS levels, and inflammation. In addition, the dampening effects of silencing Tlr4 on inflammatory pathways were verified by the enrichment analysis of RNA-sequencing data in METH-treated Tlr4−/− mice compared to METH-treated wild-type mice. Taken together, these findings implied that Tlr4 silencing, comparable to antibiotic pretreatment, effectively alleviated METH-induced hepatotoxicity by inhibiting LPS-TLR4-mediated inflammation in the liver.