bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of molecular sequence data completeness on HIV cluster detection and a network science approach to enhance detection
Authors: Mazrouee Sepideh , Hallmark Camden J. , Mora Ricardo , Del Vecchio Natascha , Carrasco Hernandez Rocio , Carr Michelle , McNeese Marlene , Fujimoto Kayo , Wertheim Joel O.
Published: November 2022

Detection of viral transmission clusters using molecular epidemiology is critical to the response pillar of the Ending the HIV Epidemic initiative. Here, we studied whether inference with an incomplete dataset would influence the accuracy of the reconstructed molecular transmission network. We analyzed viral sequence data available from ~ 13,000 individuals with diagnosed HIV (2012–2019) from Houston Health Department surveillance data with 53% completeness (n = 6852 individuals with sequences). We extracted random subsamples and compared the resulting reconstructed networks versus the full-size network. Increasing simulated completeness was associated with an increase in the number of detected clusters. We also subsampled based on the network node influence in the transmission of the virus where we measured Expected Force (ExF) for each node in the network. We simulated the removal of nodes with the highest and then lowest ExF from the full dataset and discovered that 4.7% and 60% of priority clusters were detected respectively. These results highlight the non-uniform impact of capturing high influence nodes in identifying transmission clusters. Although increasing sequence reporting completeness is the way to fully detect HIV transmission patterns, reaching high completeness has remained challenging in the real world. Hence, we suggest taking a network science approach to enhance performance of molecular cluster detection, augmented by node influence information.
Self-charging of sprays
Authors: Kooij Stefan , van Rijn Cees , Ribe Neil , Bonn Daniel
Published: November 2022

The charging of poorly conducting liquids due to flows is a well-known phenomenon, yet the precise charging mechanism is not fully understood. This is especially relevant for sprays, where the spray plume dynamics and maximum distance travelled of a spray dramatically changes for different levels of charging: charging of the droplets makes them repel, thereby preventing drop coalescence and altering the shape of the spray plume. As the charging depends on many factors including the flow and the interactions between the liquid and the nozzle, many models and scaling laws exist in the literature. In this work we focus on perhaps the simplest flow regime, laminar jets created by ultra short channels, and quantify the charging as a function of the different parameters. We present a simple model that collapses all the data for over 4 orders of magnitude difference in streaming currents for various nozzle sizes, flow velocities and surface treatments. We further show that the charging polarity can even be reversed by applying an oppositely charged coating to the nozzle, an important step for any application.
Deception about study purpose does not affect participant behavior
Authors: Rahwan Zoe , Fasolo Barbara , Hauser Oliver P.
Published: November 2022

The use of deception in research is divisive along disciplinary lines. Whereas psychologists argue that deception may be necessary to obtain unbiased measures, economists hold that deception can generate suspicion of researchers, invalidating measures and ‘poisoning’ the participant pool for others. However, experimental studies on the effects of deception, notably false-purpose deception—the most common form of experimental deception—are scarce. Challenges with participant attrition and avoiding confounds with a form of deception in which two related studies are presented as unrelated likely explain this scarcity. Here, we avoid these issues, testing within an experiment to what extent false-purpose deception affects honesty. We deploy two commonly used incentivized measures of honesty and unethical behavior: coin-flip and die-roll tasks. Across two pre-registered studies with over 2000 crowdsourced participants, we found that false-purpose deception did not affect honesty in either task, even when we deliberately provoked suspicion of deception. Past experience of deception also had no bearing on honesty. However, incentivized measures of norms indicated that many participants had reservations about researcher use of false-purpose deception in general—often considered the least concerning form of deception. Together, these findings suggest that while false-purpose deception is not fundamentally problematic in the context of measuring honesty, it should only be used as a method of last resort. Our results motivate further experimental research to study the causal effects of other forms of deception, and other potential spillovers.
Comparisons of healthy human brain temperature predicted from biophysical modeling and measured with whole brain MR thermometry
Authors: Sung Dongsuk , Risk Benjamin B. , Kottke Peter A. , Allen Jason W. , Nahab Fadi , Fedorov Andrei G. , Fleischer Candace C.
Published: November 2022

Brain temperature is an understudied parameter relevant to brain injury and ischemia. To advance our understanding of thermal dynamics in the human brain, combined with the challenges of routine experimental measurements, a biophysical modeling framework was developed to facilitate individualized brain temperature predictions. Model-predicted brain temperatures using our fully conserved model were compared with whole brain chemical shift thermometry acquired in 30 healthy human subjects (15 male and 15 female, age range 18–36 years old). Magnetic resonance (MR) thermometry, as well as structural imaging, angiography, and venography, were acquired prospectively on a Siemens Prisma whole body 3 T MR scanner. Bland–Altman plots demonstrate agreement between model-predicted and MR-measured brain temperatures at the voxel-level. Regional variations were similar between predicted and measured temperatures (< 0.55 °C for all 10 cortical and 12 subcortical regions of interest), and subcortical white matter temperatures were higher than cortical regions. We anticipate the advancement of brain temperature as a marker of health and injury will be facilitated by a well-validated computational model which can enable predictions when experiments are not feasible.
Size dependence- and induced transformations- of fractional quantum Hall effects under tilted magnetic fields
Authors: Wijewardena U. Kushan , Nanayakkara Tharanga R. , Kriisa Annika , Reichl Christian , Wegscheider Werner , Mani Ramesh G.
Published: November 2022

Two-dimensional electron systems subjected to high transverse magnetic fields can exhibit Fractional Quantum Hall Effects (FQHE). In the GaAs/AlGaAs 2D electron system, a double degeneracy of Landau levels due to electron-spin, is removed by a small Zeeman spin splitting, $$g \mu _B B$$

g

μ
B

B

, comparable to the correlation energy. Then, a change of the Zeeman splitting relative to the correlation energy can lead to a re-ordering between spin polarized, partially polarized, and unpolarized many body ground states at a constant filling factor. We show here that tuning the spin energy can produce fractionally quantized Hall effect transitions that include both a change in $$\nu$$
ν
for the $$R_{xx}$$

R

xx

minimum, e.g., from $$\nu = 11/7$$

ν
=
11
/
7

to $$\nu = 8/5$$

ν
=
8
/
5

, and a corresponding change in the $$R_{xy}$$

R

xy

, e.g., from $$R_{xy}/R_{K} = (11/7)^{-1}$$

R

xy

/

R
K

=

(
11
/
7
)

-
1

to $$R_{xy}/R_{K} = (8/5)^{-1}$$

R

xy

/

R
K

=

(
8
/
5
)

-
1

, with increasing tilt angle. Further, we exhibit a striking size dependence in the tilt angle interval for the vanishing of the $$\nu = 4/3$$

ν
=
4
/
3

and $$\nu = 7/5$$

ν
=
7
/
5

resistance minima, including “avoided crossing” type lineshape characteristics, and observable shifts of $$R_{xy}$$

R

xy

at the $$R_{xx}$$

R

xx

minima- the latter occurring for $$\nu = 4/3, 7/5$$

ν
=
4
/
3
,
7
/
5

and the 10/7. The results demonstrate both size dependence and the possibility, not just of competition between different spin polarized states at the same $$\nu$$
ν
and $$R_{xy}$$

R

xy

, but also the tilt- or Zeeman-energy-dependent- crossover between distinct FQHE associated with different Hall resistances.
A retrospective analysis of the relationship between anti-cyclic citrullinated peptide antibody and interstitial lung disease in systemic sclerosis
Authors: Ha Jang Woo , Hong Yoo Jin , Cha Hyun Jin , Moon Jeonghun Daniel , Pyo Jung Yoon , Lee Sang-Won , Park Yong-Beom , Park Chul Hwan , Song Jason Jungsik
Published: November 2022

Anti-cyclic citrullinated peptide antibody testing is used to diagnose rheumatoid arthritis and associated with interstitial lung disease in RA. Herein, we investigate the relationship between anti-CCP antibody and ILD in SSc. We performed a retrospective analysis at a tertiary medical center between 2005 and 2019. Patients with SSc, systemic lupus erythematosus, and polymyositis/dermatomyositis (PM/DM) were evaluated for anti-CCP antibody and ILD. Additionally, medical records of SSc patients with ILD were reviewed. SSc patients had the highest anti-CCP antibody positivity rate compared to those with SLE and PM/DM. The incidence of ILD was higher in SSc patients with anti-CCP antibody than in those without. The usual interstitial pneumonia (UIP) incidence was higher in the anti-CCP antibody-positive group than in the anti-CCP antibody-negative group. The DLCO was lower in the anti-CCP antibody-positive group than in the anti-CCP antibody-negative group. On multivariable analysis, factors associated with SSc-ILD were anti-CCP antibody or rheumatoid factor (β coefficient, 2.652 [95% CI 1.472 to 4.776]) and anti-Scl70 antibody (β coefficient, 4.011 [95% CI 2.142 to 7.508]). Anti-CCP antibody may be associated with a higher incidence of ILD in SSc. SSc patients with anti-CCP antibody may have more UIP pattern and lower DLCO.

Trial Registration Retrospectively registered.
Ceragenin CSA-13 displays high antibacterial efficiency in a mouse model of urinary tract infection
Authors: Wnorowska Urszula , Piktel Ewelina , Deptuła Piotr , Wollny Tomasz , Król Grzegorz , Głuszek Katarzyna , Durnaś Bonita , Pogoda Katarzyna , Savage Paul B. , Bucki Robert
Published: November 2022

Ceragenins (CSAs) are synthetic, lipid-based molecules that display activities of natural antimicrobial peptides. Previous studies demonstrated their high in vitro activity against pathogens causing urinary tract infections (UTIs), but their efficiency in vivo was not explored to date. In this study, we aimed to investigate the bactericidal efficiency of ceragenins against E. coli (Xen14 and clinical UPEC strains) isolates both in vitro and in vivo, as well to explore CSA-13 biodistribution and ability to modulate nanomechanical alterations of infected tissues using animal model of UTI. CSA-44, CSA-131 and particularly CSA-13 displayed potent bactericidal effect against tested E. coli strains, and this effect was mediated by induction of oxidative stress. Biodistribution studies indicated that CSA-13 accumulates in kidneys and liver and is eliminated with urine and bile acid. We also observed that ceragenin CSA-13 reverses infection-induced alterations in mechanical properties of mouse bladders tissue, which confirms the preventive role of CSA-13 against bacteria-induced tissue damage and potentially promote the restoration of microenvironment with biophysical features unfavorable for bacterial growth and spreading. These data justify the further work on employment of CSA-13 in the treatment of urinary tract infections.
Functional and molecular dissection of HCMV long non-coding RNAs
Authors: Lee Sungwon , Kim Hyewon , Hong Ari , Song Jaewon , Lee Sungyul , Kim Myeonghwan , Hwang Sung-yeon , Jeong Dongjoon , Kim Jeesoo , Son Ahyeon , Lee Young-suk , Kim V. Narry , Kim Jong-seo , Chang Hyeshik , Ahn Kwangseog
Published: November 2022

Small, compact genomes confer a selective advantage to viruses, yet human cytomegalovirus (HCMV) expresses the long non-coding RNAs (lncRNAs); RNA1.2, RNA2.7, RNA4.9, and RNA5.0. Little is known about the function of these lncRNAs in the virus life cycle. Here, we dissected the functional and molecular landscape of HCMV lncRNAs. We found that HCMV lncRNAs occupy ~ 30% and 50–60% of total and poly(A)+viral transcriptome, respectively, throughout virus life cycle. RNA1.2, RNA2.7, and RNA4.9, the three abundantly expressed lncRNAs, appear to be essential in all infection states. Among these three lncRNAs, depletion of RNA2.7 and RNA4.9 results in the greatest defect in maintaining latent reservoir and promoting lytic replication, respectively. Moreover, we delineated the global post-transcriptional nature of HCMV lncRNAs by nanopore direct RNA sequencing and interactome analysis. We revealed that the lncRNAs are modified with N6-methyladenosine (m6A) and interact with m6A readers in all infection states. In-depth analysis demonstrated that m6A machineries stabilize HCMV lncRNAs, which could account for the overwhelming abundance of viral lncRNAs. Our study lays the groundwork for understanding the viral lncRNA–mediated regulation of host-virus interaction throughout the HCMV life cycle.
The antipsychotic drug sulpiride in the ventral pallidum paradoxically impairs learning and induces place preference
Authors: Dusa Daniella , Ollmann Tamás , Kállai Veronika , Lénárd László , Kertes Erika , Berta Beáta , Szabó Ádám , László Kristóf , Gálosi Rita , Zagoracz Olga , Karádi Zoltán , Péczely László
Published: November 2022

Sulpiride, as a D2-like dopamine (DA) receptor (D2R) antagonist, is an important antipsychotic drug in the treatment of schizophrenia. Recently, we have shown that the activation of D2Rs in the ventral pallidum (VP) modulates the activity of the ventral tegmental area (VTA) DAergic neurons. According to our hypothesis, intra-VP sulpiride can influence the motivational and learning processes, pervasively modifying the behavior of examined animals. In the present study, sulpiride was microinjected into the VP of male Wistar rats in three different doses. Morris water maze (MWM) test was applied to investigate the effects of sulpiride on spatial learning, while conditioned place preference (CPP) test was used to examine the potential rewarding effect of the drug. In order to show, whether the animals can associate the rewarding effect with an area which can be recognized only on its spatial location, we introduced a modified version of the CPP paradigm, the spatial CPP test. Our results show that the intra-VP sulpiride dose-dependently impairs learning processes. However, the largest dose of sulpiride induces place preference. Results of the spatial CPP paradigm demonstrate that the animals cannot associate the rewarding effect of the drug with the conditioning area based on its spatial location. In the CPP paradigm, locomotor activity decrease could be observed in the sulpiride-treated rats, likely because of a faster habituation with the conditioning environment. In summary, we can conclude that intra-VP sulpiride has a dual effect: it diminishes the hippocampus-dependent spatial learning processes, in addition, it has a dose-dependent rewarding effect.
Encapsulated salts in velvet worm slime drive its hardening
Authors: Corrales-Ureña Yendry Regina , Schwab Fabienne , Ochoa-Martínez Efraín , Benavides-Acevedo Miguel , Vega-Baudrit José , Pereira Reinaldo , Rischka Klaus , Noeske Paul-Ludwig Michael , Gogos Alexander , Vanhecke Dimitri , Rothen-Rutishauser Barbara , Petri-Fink Alke
Published: November 2022

Slime expelled by velvet worms entraps prey insects within seconds in a hardened biopolymer network that matches the mechanical strength of industrial polymers. While the mechanic stimuli-responsive nature and building blocks of the polymerization are known, it is still unclear how the velvet worms’ slime hardens so fast. Here, we investigated the slime for the first time, not only after, but also before expulsion. Further, we investigated the slime’s micro- and nanostructures in-depth. Besides the previously reported protein nanoglobules, carbohydrates, and lipids, we discovered abundant encapsulated phosphate and carbonate salts. We also detected CO2 bubbles during the hardening of the slime. These findings, along with further observations, suggest that the encapsulated salts in expelled slime rapidly dissolve and neutralize in a baking-powder-like reaction, which seems to accelerate the drying of the slime. The proteins’ conformation and aggregation are thus influenced by shear stress and the salts’ neutralization reaction, increasing the slime’s pH and ionic strength. These insights into the drying process of the velvet worm’s slime demonstrate how naturally evolved polymerizations can unwind in seconds, and could inspire new polymers that are stimuli-responsive or fast-drying under ambient conditions.
89Zr-panitumumab PET imaging for preoperative assessment of ameloblastoma in a PDX model
Authors: Stone Logan D. , Massicano Adriana V. F. , Stevens Todd M. , Warram Jason M. , Morlandt Anthony B. , Lapi Suzanne E. , Amm Hope M.
Published: November 2022

Accurate assessment of tumor margins with specific, non-invasive imaging would result in the preservation of healthy tissue and improve long-term local tumor control, thereby reducing the risk of recurrence. Overexpression of epidermal growth factor receptor (EGFR) has been used in other cancers as an imaging biomarker to identify cancerous tissue. We hypothesize that expression of EGFR in ameloblastomas may be used to specifically visualize tumors. The aims of this study are to measure the specificity of radiolabeled 89Zr-panitumumab (an EGFR antibody) in vivo using patient-derived xenograft (PDX) models of ameloblastoma and positron emission tomography/computed tomography (PET/CT) scans. In PDX of ameloblastomas from four patients (AB-36, AB-37, AB-39 AB-53), the biodistribution of 89Zr-panitumumab was measured 120 h post-injection and was reported as the injected dose per gram of tissue (%ID/g; AB-36, 40%; AB-37, 62%; AB-39 18%; AB-53, 65%). The radiolabeled %ID/g was significantly greater in tumors of 89Zr-panitumumab-treated mice that did not receive unlabeled panitumumab as a blocking control for AB-36, AB-37, and AB-53. Radiolabeled anti-EGFR demonstrates specificity for ameloblastoma PDX tumor xenografts, we believe 89Zr-panitumumab is an attractive target for pre-surgical imaging of ameloblastomas. With this technology, we could more accurately assess tumor margins for the surgical removal of ameloblastomas.
Sugarcane cultivation practices modulate rhizosphere microbial community composition and structure
Authors: Moneda Ana Paula Corrêa , de Carvalho Lucas Amoroso Lopes , Teheran-Sierra Luis Guillermo , Funnicelli Michelli Inácio Gonçalves , Pinheiro Daniel Guariz
Published: November 2022

Sugarcane (Saccharum spp.) represents a crop of great economic importance, remarkably relevant in the food industry and energy supply chains from renewable sources. However, its conventional cultivation involves the intensive use of fertilizers, pesticides, and other agrochemical agents whose detrimental effects on the environment are notorious. Alternative systems, such as organic farming, have been presented as an environmentally friendly way of production. Still, the outcomes of different cropping systems on the microbiota associated with sugarcane—whose role in its health and growth is crucial—remain underexplored. Thus, we studied the rhizospheric microbiota of two adjacent sugarcane fields, which differ in terms of the type of farming system. For this, we used the sequencing of taxonomic markers of prokaryotes (gene 16S rRNA, subregions V3–V4) and fungi (Internal transcribed spacer 2) and evaluated the changes caused by the systems. Our results show a well-conserved microbiota composition among farming systems in the highest taxonomic ranks, such as phylum, class, and order. Also, both systems showed very similar alpha diversity indices and shared core taxa with growth-promoting capacities, such as bacteria from the Bacillus and Bradyrhizobium genera and the fungal genus Trichoderma. However, the composition at more specific levels denotes differences, such as the separation of the samples concerning beta diversity and the identification of 74 differentially abundant taxa between the systems. Of these, 60 were fungal taxa, indicating that this microbiota quota is more susceptible to changes caused by farming systems. The analysis of co-occurrence networks also showed the formation of peripheral sub-networks associated with the treatments—especially in fungi—and the presence of keystone taxa in terms of their ability to mediate relationships between other members of microbial communities. Considering that both crop fields used the same cultivar and had almost identical soil properties, we conclude that the observed findings are effects of the activities intrinsic to each system and can contribute to a better understanding of the effects of farming practices on the plant microbiome.
Resolving the bouba-kiki effect enigma by rooting iconic sound symbolism in physical properties of round and spiky objects
Authors: Fort Mathilde , Schwartz Jean-Luc
Published: November 2022

The “bouba-kiki effect”, where “bouba” is perceived round and “kiki” spiky, remains a puzzling enigma. We solve it by combining mathematical findings largely unknown in the field, with computational models and novel experimental evidence. We reveal that this effect relies on two acoustic cues: spectral balance and temporal continuity. We demonstrate that it is not speech-specific but rather rooted in physical properties of objects, creating audiovisual regularities in the environment. Round items are mathematically bound to produce, when hitting or rolling on a surface, lower-frequency spectra and more continuous sounds than same-size spiky objects. Finally, we show that adults are sensitive to such regularities. Hence, intuitive physics impacts language perception and possibly language acquisition and evolution too.
Differences in diagnosis, management, and outcomes of acute febrile illness by health facility level in southern Ethiopia
Authors: Shimelis Techalew , Vaz Nery Susana , Schierhout Gill , Tadesse Birkneh Tilahun , Dittrich Sabine , Crump John A. , Kaldor John M.
Published: November 2022

We assessed the diagnosis, management and outcomes of acute febrile illness in a cohort of febrile children aged under 5 years presenting at one urban and two rural health centres and one tertiary hospital between 11 August 2019 and 01 November 2019. Pneumonia was diagnosed in 104 (30.8%) of 338 children at health centres and 128 (65.0%) of 197 at the hospital (p < 0.001). Malaria was detected in 33 (24.3%) of 136 children at the urban health centre, and in 55 (55.6%) of 99 and 7 (7.4%) of 95 children at the rural health centres compared to 11 (11.6%) of 95 at the hospital. Antibacterials were prescribed to 20 (11.5%) of 174 children without guidelines-specified indications (overprescribing) at health centres and in 7 (33.3%) of 21 children at the hospital (p = 0.013). Antimalarials were overprescribed to 13 (7.0%) of 185 children with negative malaria microscopy at the hospital. The fever resolved by day 7 in 326 (99.7%) of 327 children at health centres compared to 177 (93.2%) of 190 at the hospital (p < 0.001). These results suggest that additional guidance to health workers is needed to optimise the use of antimicrobials across all levels of health facilities.
An optically-guided cochlear implant sheath for real-time monitoring of electrode insertion into the human cochlea
Authors: Starovoyt Anastasiya , Quirk Bryden C. , Putzeys Tristan , Kerckhofs Greet , Nuyts Johan , Wouters Jan , McLaughlin Robert A. , Verhaert Nicolas
Published: November 2022

In cochlear implant surgery, insertion of perimodiolar electrode arrays into the scala tympani can be complicated by trauma or even accidental translocation of the electrode array within the cochlea. In patients with partial hearing loss, cochlear trauma can not only negatively affect implant performance, but also reduce residual hearing function. These events have been related to suboptimal positioning of the cochlear implant electrode array with respect to critical cochlear walls of the scala tympani (modiolar wall, osseous spiral lamina and basilar membrane). Currently, the position of the electrode array in relation to these walls cannot be assessed during the insertion and the surgeon depends on tactile feedback, which is unreliable and often comes too late. This study presents an image-guided cochlear implant device with an integrated, fiber-optic imaging probe that provides real-time feedback using optical coherence tomography during insertion into the human cochlea. This novel device enables the surgeon to accurately detect and identify the cochlear walls ahead and to adjust the insertion trajectory, avoiding collision and trauma. The functionality of this prototype has been demonstrated in a series of insertion experiments, conducted by experienced cochlear implant surgeons on fresh-frozen human cadaveric cochleae.
Coastal upwelling generates cryptic temperature refugia
Authors: Salois Sarah L. , Gouhier Tarik C. , Helmuth Brian , Choi Francis , Seabra Rui , Lima Fernando P.
Published: November 2022

Understanding the effects of climate-mediated environmental variation on the distribution of organisms is critically important in an era of global change. We used wavelet analysis to quantify the spatiotemporal (co)variation in daily water temperature for predicting the distribution of cryptic refugia across 16 intertidal sites that were characterized as ‘no’, ‘weak’ or ‘strong’ upwelling and spanned 2000 km of the European Atlantic Coast. Sites experiencing weak upwelling exhibited high synchrony in temperature but low levels of co-variability at monthly to weekly timescales, whereas the opposite was true for sites experiencing strong upwelling. This suggests upwelling generates temporal thermal refugia that can promote organismal performance by both supplying colder water that mitigates thermal stress during hot Summer months and ensuring high levels of fine-scale variation in temperature that reduce the duration of thermal extremes. Additionally, pairwise correlograms based on the Pearson-product moment correlation coefficient and wavelet coherence revealed scale dependent trends in temperature fluctuations across space, with a rapid decay in strong upwelling sites at monthly and weekly timescales. This suggests upwelling also generates spatial thermal refugia that can ‘rescue’ populations from unfavorable conditions at local and regional scales. Overall, this study highlights the importance of identifying cryptic spatiotemporal refugia that emerge from fine-scale environmental variation to map potential patterns of organismal performance in a rapidly changing world.
Bias in the association between advanced maternal age and stillbirth using left truncated data
Authors: Dunne Jennifer , Tessema Gizachew A. , Gebremedhin Amanuel T. , Pereira Gavin
Published: November 2022

Restriction to analysis of births that survive past a specified gestational age (typically 20 weeks gestation) leads to biased exposure-outcome associations. This bias occurs when the cause of restriction (early pregnancy loss) is influenced by both the exposure and unmeasured factors that also affect the outcome. The aim of this study is to estimate the magnitude of bias resulting from left truncated data in the association between advanced maternal age and stillbirth. We simulated data for the causal pathway under a collider-stratification mechanism. Simulation parameters were based on an observed birth cohort from Western Australia and a range of plausible values for the prevalence of early pregnancy loss, unmeasured factor U and the odds ratios for the selection effects. Selection effects included the effects of maternal age on early pregnancy loss, U on early pregnancy loss, and U on stillbirth. We compared the simulation scenarios to the observed birth cohort that was truncated to pregnancies that survived beyond 20 gestational weeks. We found evidence of marginal downward bias, which was most prominent for women aged 40 + years. Overall, we conclude that the magnitude of bias due to left truncation is minimal in the association between advanced maternal age and stillbirth.
Blockade of persistent colored isomer formation in photochromic 3H-naphthopyrans by excited-state intramolecular proton transfer
Authors: Gierczyk Błażej , Murphree S. Shaun , Rode Michał F. , Burdzinski Gotard
Published: November 2022

In photochemistry the excited-state intramolecular proton transfer process (ESIPT) is often observed as a highly efficient singlet excited state depletion pathway, which in the presence of a strong intramolecular hydrogen bond may proceed on a subpicosecond time scale. The present work describes the suppression of unwanted transoid-trans isomer formation in photochromic 3H-naphthopyran derivatives by the introduction of a 5-hydroxy substituent. According to time-resolved spectroscopy experiments and excited-state ab initio calculations, transoid-cis → transoid-trans photoisomerization is reduced by a competitive ESIPT channel in nonpolar solvent (cyclohexane). Upon specific solute–solvent interactions (methanol, acetonitrile) the intramolecular hydrogen bond in the transoid-cis form is perturbed, favoring the internal conversion S1 → S0 process as photostabilizing channel.
Resonant and non-resonant optimizations by multi-constraint quantum control theory in molecular rotational states
Authors: Li Jin-Fang , Hu Jie-Ru , Guo Qiu-Fen , He Dong-Shan
Published: November 2022

It is a promising research for optimization of quantum gate in the field of quantum computation. We investigate the feasibility of implementing the single-qubit gate (Hadamard) in molecular rotational system. By applying the Multi-constraint quantum optimal control method, the excepted final states can be achieved based on the molecular rotational states both in resonant and non-resonant cases with the control pulses. The permanent electric dipole moment is ignored in non-resonance. Besides, the zero-pulse area constraint and the constant fluence constraint are employed to optimize shapes of control pulses. Finally, we show that the Hadamard gate can be realized with the high fidelity (0.9999) and also examine the dependence of the fidelity on pulse fluence as well as the control pulse.
Modelling time-varying interactions in complex systems: the Score Driven Kinetic Ising Model
Authors: Campajola Carlo , Gangi Domenico Di , Lillo Fabrizio , Tantari Daniele
Published: November 2022

A common issue when analyzing real-world complex systems is that the interactions between their elements often change over time. Here we propose a new modeling approach for time-varying interactions generalising the well-known Kinetic Ising Model, a minimalistic pairwise constant interactions model which has found applications in several scientific disciplines. Keeping arbitrary choices of dynamics to a minimum and seeking information theoretical optimality, the Score-Driven methodology allows to extract from data and interpret the presence of temporal patterns describing time-varying interactions. We identify a parameter whose value at a given time can be directly associated with the local predictability of the dynamics and we introduce a method to dynamically learn its value from the data, without specifying parametrically the system’s dynamics. We extend our framework to disentangle different sources (e.g. endogenous vs exogenous) of predictability in real time, and show how our methodology applies to a variety of complex systems such as financial markets, temporal (social) networks, and neuronal populations.
Prognostic differences and implications on treatment strategies between butterfly glioblastoma and glioblastoma with unilateral corpus callosum infiltration
Authors: Hazaymeh Mohammad , Löber-Handwerker Ronja , Döring Katja , Abboud Tammam , Mielke Dorothee , Rohde Veit , Malinova Vesna
Published: November 2022

Approximately 25% of glioblastomas show at diagnosis a corpus callosum infiltration, which is associated with poor prognosis. The extent of corpus callosum involvement, however, ranges from partial unilateral to complete bilateral infiltration. The role of surgery in glioblastoma with corpus callosum involvement is controversial. In this study, we aimed to examine prognostic differences between glioblastoma with unilateral and glioblastoma with bilateral corpus callosum infiltration, and to evaluate possible treatment strategy implications. Patients with newly diagnosed glioblastoma from 2010 to 2019 were included. Corpus callosum infiltration was assessed in contrast-enhanced T1-weighted preoperative magnetic resonance imaging. Extent of resection, adjuvant treatments and overall survival were evaluated. Corpus callosum involvement was found in 96 (26.4%) out of 363 patients with newly diagnosed glioblastoma. Bilateral corpus callosum infiltration was found in 27 out of 96 patients (28%), and 69 patients had unilateral corpus callosum infiltration. Glioblastoma with corpus callosum affection had significantly lower median overall survival compared to glioblastoma without corpus callosum involvement (9 vs. 11 months, p = 0.02). A subgroup analysis of glioblastoma with unilateral corpus callosum infiltration revealed a significant difference in median overall survival dependent on extent of resection (6.5 without gross total resection vs. 11 months with gross total resection, Log-rank test p = 0.02). Our data confirms a shorter overall survival in glioblastoma subpopulation with corpus callosum involvement, especially for glioblastoma with bilateral corpus callosum infiltration. However, patients with partial corpus callosum infiltration undergoing gross total resection exhibited a significant survival benefit compared to their counterparts without gross total resection. Whenever reasonably achievable gross total resection should be considered as an integral part of the treatment strategy in glioblastoma with partial corpus callosum infiltration.
Measurement and analysis of the longitudinal level irregularity of the track beam in monorail tour-transit systems
Authors: Guo Fengqi , Wang Pengjiao
Published: November 2022

Notable effects on the vertical dynamic response of vehicle-bridge systems are introduced by longitudinal level irregularity (LLI). However, in monorail systems, the measured data and associated spectrum of the LLI, i.e., the distribution curve of power spectral density versus wavelength, have not been reported in detail. To address this issue, we propose the inclination correction method (ICM) to measure the LLI in monorail tour-transit systems and further estimate and fit the spectral curves for the first time. The measuring principle of ICM is thoroughly described, and ICM is compared with conventional chord-based methods. In addition, the trend components of the measured LLI are eliminated through an adaptive method preceding spectral estimation to avoid potential errors. Notably, a simulation program is designed, and the results from existing methods are adopted to verify the proposed ICM. Based on an analysis of the results, the accuracy and robustness of ICM are demonstrated, and the applicability and advantages of autoregressive models and the proposed fractional functions in spectral analysis are revealed. Finally, combining qualitative and quantitative calculations, an evaluation approach for the generic spectra of monorail LLI is established.
High-throughput production of LuAG-based highly luminescent thick film scintillators for radiation detection and imaging
Authors: Matsumoto Shogen , Ito Akihiko
Published: November 2022

Radiography is non-destructive imaging for engineering, medical diagnostics, airport security checks, and decontamination activities in nuclear plants. Inorganic scintillators are phosphor materials that convert radiation into visible photons with high luminescence and fast response, and scintillators with a few tens of micrometers thickness can improve sensitivity in radiation detection and imaging. To date, a production method for thick film scintillators is a time and cost consuming way of slicing and poshing bulk single crystals and transparent ceramics. Here, the chemically vapor deposited Ce3+-doped Lu3Al5O12 thick film scintillators (CVD-Ce3+:LuAG) with a thickness of 1–25 μm were produced at deposition time of 1–30 min. Numerical simulations indicated the penetration depth of α-particle in Ce3+:LuAG is 12.8 μm, and the 14-μm-thick CVD-Ce3+:LuAG showed highest light yield (31,000 photons 5.5 MeV−1), superior to the commercial Ce3+:LuAG single crystal scintillator (21,000 photons 5.5 MeV−1). In the X-ray radiograph taken with CVD-Ce3+:LuAG as a scintillation screen, 5-μm-width bar of metal microgrids can be identified. Vapor deposition technique can be a novel high-throughput production way of a thick film scintillator which is in a micrometer-thickness effective to converting radiations into photons for sensitive α-emitter detection and high-resolution X-ray imaging.
Effects of predictable behavioral patterns on anxiety dynamics
Authors: Lang Martin , Krátký Jan , Xygalatas Dimitris
Published: November 2022

People face stressors that are beyond their control and that maladaptively perpetuate anxiety. In these contexts, rituals emerge as a natural coping strategy helping decrease excessive anxiety. However, mechanisms facilitating these purported effects have rarely been studied. We hypothesized that repetitive and rigid ritual sequences help the human cognitive-behavioral system to return to low-entropy states and assuage anxiety. This study reports a pre-registered test of this hypothesis using a Czech student sample (n = 268). Participants were exposed to an anxiety induction and then randomly assigned to perform one of three actions: ritualized, control, and neutral (no-activity). We assessed the effects of this manipulation on cognitive and physiological anxiety, finding that ritualized action positively affected anxiety decrease, but this decrease was only slightly larger than in the other two conditions. Nevertheless, the between-condition differences in the reduction of physiological anxiety were well-estimated in participants more susceptible to anxiety induction.
Site-specific temporal variation of population dynamics in subalpine endemic plant species
Authors: Jeong Hyungsoon , Cho Yong-Chan , Kim Eunsuk
Published: November 2022

Endemic plants in high mountains are projected to be at high risk because of climate change. Temporal demographic variation is a major factor affecting population viability because plants often occur in small, isolated populations. Because isolated populations tend to exhibit genetic differentiation, analyzing temporal demographic variation in multiple populations is required for the management of high mountain endemic species. We examined the population dynamics of an endemic plant species, Primula farinosa subsp. modesta, in four subalpine sites over six years. Stage-based transition matrices were constructed, and temporal variation in the projected population growth rate (λ) was analyzed using life table response experiments (LTREs). The variation in λ was primarily explained by the site × year interaction rather than the main effects of the site and year. The testing sites exhibited inconsistent patterns in the LTRE contributions of the vital rates to the temporal deviation of λ. However, within sites, growth or stasis had significant negative correlations with temporal λ deviation. Negative correlations among the contributions of vital rates were also detected within the two testing sites, and the removal of the correlations alleviated temporal fluctuations in λ. The response of vital rates to yearly environmental fluctuations reduced the temporal variation of λ. Such effects manifested especially at two sites where plants exhibited higher plasticity than plants at other sites. Site-specific temporal variation implies that populations of high mountain species likely exhibit asynchronous temporal changes, and multiple sites need to be evaluated for their conservation.
Accuracy and reliability of the optoelectronic plethysmography and the heart rate systems for measuring breathing rates compared with the spirometer
Authors: Stubbe Laurent , Houel Nicolas , Cottin François
Published: November 2022

Measuring breathing rates without a mouthpiece is of interest in clinical settings. Electrocardiogram devices and, more recently, optoelectronic plethysmography (OEP) methods can estimate breathing rates with only a few electrodes or motion-capture markers placed on the patient. This study estimated and compared the accuracy and reliability of three non-invasive devices: an OEP system with 12 markers, an electrocardiogram device and the conventional spirometer. Using the three devices simultaneously, we recorded 72 six-minute epochs on supine subjects. Our results show that the OEP system has a very low limit of agreement and a bias lower than 0.4% compared with the spirometer, indicating that these devices can be used interchangeably. We observed comparable results for electrocardiogram devices. The OEP system facilitates breathing rate measurements and offers a more complete chest-lung volume analysis that can be easily associated with heart rate analysis without any synchronisation process, for useful features for clinical applications and intensive care.
Exploitation of cantaloupe peels for bacterial cellulose production and functionalization with green synthesized Copper oxide nanoparticles for diverse biological applications
Authors: Saleh Ahmed K. , El-Gendi Hamada , El-Fakharany Esmail M. , Owda Medhat E. , Awad Mohamed A. , Kamoun Elbadawy A.
Published: November 2022

The promising features of most bacterial celluloses (BC) promote the continuous mining for a cost-effective production approach toward wide and sustainable applications. Herein, cantaloupe peels (CP) were successfully implemented for sustainable BC production. Results indicated that the enzymatically hydrolyzed CP supported the maximum BC production of approximately 3.49 g/L when used as a sole fermentation media. The produced BC was fabricated with polyvinyl alcohol (PVA) and chitosan (Ch), and loaded with green synthesized copper oxide nanoparticles (CuO-NPs) to improve its biological activity. The novel composite showed an antimicrobial activity against several human pathogens such asStaphylococcus aureus, Streptococcus mutans, Salmonella typhimurium, Escherichia coli,andPseudomonas fluorescens. Furthermore, the new composite revealed a significant in vitro anticancer activity against colon (Caco-2), hepatocellular (HepG-2), and breast (MDA) cancer cells, with low IC50of 0.48, 0.27, and 0.33 mg/mL for the three cell lines, respectively. On the other hand, the new composite was remarkably safe for human skin fibroblast (HSF) with IC50of 1.08 mg/mL. Interestingly, the composite membranes exhibited lethal effects against all stages of larval instar and pupal stage compared with the control. In this study, we first report the diverse potential applications of BC/PVA/Ch/CuO-NPs composites based on green synthesized CuO-NPs and sustainably produced BC membrane.
Stunting among children aged 24–59 months and associations with sanitation, enteric infections, and environmental enteric dysfunction in rural northwest Ethiopia
Authors: Gizaw Zemichael , Yalew Alemayehu Worku , Bitew Bikes Destaw , Lee Jiyoung , Bisesi Michael
Published: November 2022

Stunting is a public health issue of global concern. Despite, poor sanitation, diarrhea, parasitic infections, and environmental enteric dysfunction (EED) are associated with stunting, their link is poorly understood and has not been investigated in Ethiopia. This study was conducted to assess the associations of stunting with sanitation, enteric infections, and EED among children aged 24–59 months in rural northwest Ethiopia. A community-based cross-sectional study was conducted among 224 randomly selected children aged 24–59 months in rural areas of the east Dembiya district. We collected information on household food insecurity and dietary diversity using pre-tested questionnaires adopted from the food and nutrition technical assistance (FANTA) project. We used height-for-age-z score (HAZ) to define stunting. We also used the data collected to measure the environmental exposures of children to intestinal parasitic infections and fecal biomarkers of EED. A multivariable binary logistic regression model was used to assess the association of stunting with sanitation, enteric infections, and EED. Of the 224 children, 33% (95% CI 27, 39%) were stunted. Stunting in children was significantly associated with poor dietary intake (AOR 3.0, 95% CI 1.2, 7.3), open defecation practice (AOR 3.0, 95% CI 1.2, 7.9), presence of animal excreta in the living environment (AOR 3.4, 95% CI 1.2, 9.9), E. coli contamination of drinking water (AOR 4.2, 95% CI 1.1, 15.3), diarrheal disease incidence (AOR 3.4, 95% CI 1.5, 7.7), intestinal parasites in children (AOR 3.3, 95% CI 1.3, 8.8), and higher EED disease activity scores (AOR 2.9, 95% CI 1.2, 6.7). One-third of the children in the study area were stunted and this high prevalence of stunting was associated with poor dietary intake, poor hygiene and sanitation conditions, enteric infections, and EED. Thus, stunting can be prevented by improving sanitation and hygienic conditions to prevent repeated enteric infections in children and by promoting dietary diversity of children.
The effect of preeclampsia on adverse maternal outcomes in Sidama region, Ethiopia: a prospective open cohort study
Authors: Jikamo Birhanu , Adefris Mulat , Azale Telake , Alemu Kassahun
Published: November 2022

Ethiopia has made improvements in the reduction of maternal mortality; the high burden of preeclampsia remains a concern in the Sidama region. This study aimed to measure the effect of preeclampsia on adverse maternal outcomes and identify risk factors among women with preeclampsia in Sidama region. A prospective open cohort study was conducted from August 8, 2019, to October 1, 2020. We enrolled a total of 1015 the pregnant women who had preeclampsia and normotensive women at ≥ 20 weeks of gestation and followed them until 42 days after delivery. A log-binomial logistic regression model was used to estimate the incidence of adverse maternal outcomes and its risk factors. There were 276 adverse maternal outcomes observed in the preeclampsia group compared to 154 adverse maternal outcomes in the normotensive group (P < 0.001). Women with severe features of preeclampsia had a 43% (aRR = 1.43, 95% CI 1.3–1.58) higher risk for adverse maternal outcomes compared to women without severe features of preeclampsia. Women without severe features of preeclampsia had a 39% (aRR = 1.39, 95% CI 1.2–1.76) higher risk for adverse maternal outcomes compared to women in the normotensive group. More adverse maternal outcomes occurred among women with preeclampsia after controlling for confounders.