bluuebunny/crossref_metadata_2025 huggingface dataset:
Guideline for genome transposon annotation derived from evaluation of popular TE identification tools
Authors: Yan Haidong , Torchiana Federica , Bombarely Aureliano
Published: August 2020

Background: Transposable elements (TEs) constitute the vast majority of all eukaryotic DNA, and display extreme diversity, with thousands of families. Given their abundance and diversity, TEs discovery and annotation becomes challengeable. At present, tools and databases have built libraries to mask TEs in genomes based on de novo- and homology-based identification strategies, but no consensus criteria about which tools should be used have been proposed.

Results: In the de novo-based strategy, we compared performances of TE libraries developed by four commonly used tools, including RepeatModeler, LTR_FINDER, LTRharvest, and MITE_Hunter, by using a simulated genome as a standard control. The results showed that the performance of RepeatModeler decreased as it was combined with either LTR_FINDER or LTRharvest. Combination of RepeatModeler and MITE_Hunter showed better performance than RepeatModeler and MITE_Hunter alone. In the homology-based strategy, we evaluated different sources from a taxonomic point of view to build an accurate TE library. When we selected a library from databases to identify TEs for Arabidopsis thaliana genome, the library from a genus genetically closer to Arabidopsis achieved better performance than other genera with further genetic distance. Without the Arabidopsis, combination of top three genera closer to Arabidopsis showed better performance than combination of all genera.

Conclusion: This study proposes a series of recommendations to perform an accurate TE annotation: 1) For de novo-based strategy, RepeatModeler and MITE_Hunter are suggested to build a TE library; 2) For homology-based strategy, it is recommended to use library of genus genetically close to the species rather than use combined library from all genera.
Winter Pruning Effect on Root Density, Root Distribution and Root/canopy Ratio in Vitis vinifera cv. Pinot Gris
Authors: Tomasi Diego , Gaiotti Federica , Petoumenou Despoina , Lovat Lorenzo , Belfiore Nicola , Mian Giovanni
Published: August 2020

As in any other plant, in the grapevine roots play a vital role in terms of anchorage, uptake of water and nutrients, as well as storage and production of chemicals. Their behaviour and development depend on various factors, namely rootstock genetics, soil physical and chemical features, field agronomic practices. Canopy management, involving techniques such as defoliation and pruning, could greatly influence root growth. To date, most of the studies on grapevine winter pruning have focused on the effects on yield and quality of grapes, achievable by using different pruning systems and techniques, while the knowledge of root distribution, development, and growth in relation to winter pruning is still not well understood. In this contest, the purpose of this study was to investigate the effect of winter pruning on the root system of field-grown Vitis vinifera cv. Pinot Gris grafted onto rootstock SO4. We compared two pruning treatments (pruning-P and no pruning-NP) and analysed the effect on root distribution and density, root index and on the root sugar reserves. Root data were analysed in relation to canopy growth and yield, to elucidate the effect of winter pruning on the root/yield ratio. Our data indicated that winter pruning stimulated the root growth and distribution without compromising canopy development, while no-pruning treatment produced less growth of roots but a larger canopy. Information regarding root growth and root canopy ratio is important as it gives us an understanding of the relationship between the aerial and subterranean parts of the plant, how they compete, and finally, offers us the possibility to ponder on cultural practices.
Ablation-Dominated Arcs in CO2 Atmosphere - Part II: Molecule Emission and Absorption
Authors: Methling Ralf , Götte Nicolas , Uhrlandt Dirk
Published: August 2020

Molecule radiation can be used as a tool to study colder regions in switching arc plasmas like arc fringes in contact to walls and ranges around current zero (CZ). This is demonstrated in the present study for the first time for the case of ablation-dominated high–current arcs as key elements of self–blast circuit breakers. The arc in a model circuit breaker (MCB) in CO2 with and an arc in a long nozzle under ambient conditions with peak currents between 5 and 10 kA were studied by emission and absorption spectroscopy in the visible spectral range. The nozzle material was polytetrafluoroethylene (PTFE) in both cases. Imaging spectroscopy was carried out either with high-speed cameras or with intensified CCD cameras. A pulsed high-intensity Xe lamp was applied as background radiator for the broad-band absorption spectroscopy. Emission of Swan bands from carbon dimers was observed at the edge of nozzles only or across the whole nozzle radius with highest intensity in the arc center, depending on current and nozzle geometry. Furthermore, absorption of C2 Swan bands and CuF bands were found with the arc plasma serving as background radiator. After CZ, only CuF was detected in absorption experiments.
Solving Prandtl-Blasius boundary layer equation using Maple
Authors: Sun Bohua
Published: August 2020

A solution for the Prandtl-Blasius equation is essential to all kinds of boundary layer problems. This paper revisits this classic problem and presents a general Maple code as its numerical solution. The solutions were obtained from the Maple code, using the Runge-Kutta method. The study also considers convergence radius expanding and an approximate analytic solution is proposed by curve fitting.
Численное моделирование пиролиза пропана в проточном химическом реакторе под воздействием постоянного внешнего нагрева
Authors: Губайдуллин Ирек Марсович , Gubaydullin Irek Marsovich , Жалнин Руслан Викторович , Zhalnin Ruslan V. , Масягин Виктор Федорович , Masyagin Victor Fedorovich , Пескова Елизавета Евгеньевна , Peskova Elizaveta Evgenievna , Тишкин Владимир Федорович , Tishkin Vladimir Fedorovich
Published: August 2020

Проведено численное моделирование процесса пиролиза пропана в проточном химическом реакторе, в котором химические превращения осуществляются за счет внешнего обогрева зоны реакции. Для математического описания исследуемых процессов используются уравнения Навье-Стокса в приближении малых чисел Маха, поскольку скорость движения газовой смеси много меньше скорости звука в данной смеси. При построении разностной схемы используется интегро-интерполяционный метод. Для решения уравнений химической кинетики использовалась специализированная явная схема второго порядка точности, обладающая малой вычислительной трудоемкостью. В качестве основы для описания химических превращений пиролиза пропана использовалась известная кинетическая схема, включающая в себя 30 элементарных стадий. Однако для более точного описания процесса была скорректирована энергия активации одной из стадий реакции. Было проведено численное моделирование процесса пиролиза пропана с учетом процессов вязкости, диффузии и теплопроводности для различных температур нагревательных элементов. Проведено сравнение полученных результатов по конверсии пропана с экспериментальными данными и известными численными результатами решения данной задачи. Сделан вывод о том, что разработанный численный алгоритм дает высокую достоверность получаемых результатов и может быть применен на практике для моделирования исследуемых процессов.
Effectiveness of Active Debate Method in English Speaking Skills of Students in SMAN 3 Padangsidimpuan
Authors: Nasution Khairunnisah , Hasibuan Apriyanti , Purba Happy Sri Rezeki
Published: August 2020

This research aimed are : 1) Knowing the effectiveness of the debate method in improving students' speaking skills in learning English, 2) Knowing the differences in students' speaking skills using the debate method and without using debate method.The data was collected using test, they were pretest and postest. The result of evaluation was analyzed through quantitative. The results showed: There was a significant difference between the speaking skills of students who received speaking learning using the active debate method with students who received speaking learning without using the active debate method The difference in speaking skills is indicated by the results of the posttest t-test of the control group and the posttest of the experimental group, namely the calculation results show that the t count score is greater than the t table score (th: 2.882> tt: 1.994) at the 5% significance level and db 70. The results of the t-test showed that there were differences in the speaking skills of the control group students who received speaking learning without using the active debate method and the experimental group that received speaking learning using the active debate method.
Pilot Test of Surfactant-Polymer Flood with Mixtures of Anionic-Cationic Surfactants for High Temperature Low Permeability Sandstone Reservoir
Authors: Li Yingcheng , Zhang Weidong , Shen Zhiqin , Jin Jun , Su Zhiqing , Yao Feng , Yu Xiaoling , Bao Xinning , He Xiujuan , Wu Xinyue , Zhang Hui , Sha Ou
Published: August 2020

The first pilot test of surfactant-polymer (SP) flood in the world with mixtures of anionic-cationic surfactants (Sa/c) was carried out for a high temperature low permeability sandstone reservoir with high content of clay to demonstrate the potential of this novel technique to improve oil recovery.

Low critical micelle concentrations (CMC) of 2.78×10−6 mol/L, ultralow interfacial tension (IFT) of 10−3 to 10−4 mN/m when surfactant concentrations were above 0.025 wt%, and lower phase microemulsion with high oil solubilization of 22, as well as 55.45 % oil washing rate were obtained by using Sa/c. The adsorption inhibitor (AI) was adopted to reduce the adsorption because of the high clay contained in the natural core. Dynamic adsorption was about 0.30 mg/g with addition of AI, as well as IFT kept almost unchanged before and after adsorption.

In order to reduce the injection pressure and improve the mobility ratio in the low permeability reservoir, low molecular weight polyacrylamide was adopted. The viscosity of polymer and SP were 2.96 mPa·s and 4.05 mPa·s, respectively. Core flooding results showed more than 16 % original-oil-in-place (OOIP) crude oil was recovered by SP over water flood.

Since August 2012, the pilot test of SP containing Sa/c was carried out in a Sinopec reservoir with temperature of 83 °C, salinity of 15,000 mg/L, permeability of 41.5 mD, clay of 12 %. Totally 0.40 PV chemicals, including 0.1 PV polymer pre-slug, 0.25 PV SP main slug and 0.05 PV polymer drive, were injected from August 2012 to December 2017 with an injection rate of 0.08 PV/a. After that water drive was conducted. Maximal water cut decreased from 82.2 % to 62.1 %, and the peak daily oil production increased from 12.2 t to 32.3 t. The oil recovery was increased by 8.0 % OOIP by the end of December 2018.
Ethanolamine as Alkali for Alkali Surfactant Polymer Flooding - Development of a Low-Complexity Field Implementation Concept
Authors: Southwick Jeffrey , Brewer Mark , van Batenburg Diederik , Pieterse Sebastiaan , Bouwmeester Ron , Mahruqi Dawood , Alkindi Abdullah , Mjeni Rifaat
Published: August 2020

A successful pilot in the Marmul field in the south of the Sultanate of Oman used a formulation consisting of 2% sodium carbonate, 0.3% surfactant, and 0.27% polymer. Marmul crude oil has a high total acid number (TAN = 0.9 mg KOH/ml). The petroleum acids are neutralized by alkali to form soaps which mix with the added surfactant to generate low interfacial tension (IFT) between oil and brine. For a commercial scale chemical-flood the quantity of sodium carbonate that needs to be transported is very large. It has been found that ethanolamine has significant advantages as an alkali for ASP and can substantially improve the logistical challenges faced in implementing a commercial scale flood. Ethanolamine has an improved alkaline density, is a low viscosity liquid, and can function as a solvent for EOR surfactants in the free acid form enabling shipment of a ready to mix liquid AS formulation.
‘Motivational work’: a qualitative study of preventive health dialogues in general practice
Authors: Broholm-Jørgensen Marie , Langkilde Siff Monrad , Tjørnhøj-Thomsen Tine , Pedersen Pia Vivian
Published: August 2020

Background: The aim of this article is to explore preventive health dialogues in general practice in the context of a pilot study of a Danish primary preventive intervention ‘TOF’ (a Danish acronym for ‘Early Detection and Prevention’) carried out in 2016. The intervention consisted of 1) a stratification of patients into one of four groups, 2) a digital support system for both general practitioners and patients, 3) an individual digital health profile for each patient, and 4) targeted preventive services in either general practice or a municipal health center.Methods: The empirical material in this study was obtained through 10 observations of preventive health dialogues conducted in general practices and 18 semi-structured interviews with patients and general practitioners. We used the concept of ‘motivational work’ as an analytical lens for understanding preventive health dialogues in general practice from the perspectives of both general practitioners and patients.Results: While the health dialogues in TOF sought to reveal patients’ motivations, understandings, and priorities related to health behavior, we find that the dialogues were treatment-oriented and structured around biomedical facts, numeric standards, and risk factor guidance. Overall, we find that numeric standards and quantification of motivation lessens the dialogue and interaction between GP and patient and that contextual factors relating to the intervention framework, such as a digital support system, the general practitioners’ perceptions of their professional position as well as the patients’ understanding of prevention —in an interplay—diminished the motivational work carried out in the health dialogues.Conclusion: The findings show that the influence of different kinds of context adds to the complexity of prevention in the clinical encounter which help to explain why motivational work is difficult in general practice.
Clients' satisfaction with preventive services for malaria during pregnancy in Anambra State, Nigeria
Authors: Obagha Chijioke Emmanuel , Ajayi IkwOluwakpo , Abdullahi Gobir A. , Umeokonkwo Chukwuma David
Published: August 2020

Background: Burden of Malaria in pregnancy (MIP) is still high despite availability of proven cost-effective interventions. Considerable progress has been made on improving antenatal attendance but MIP preventive services utilization remains low. Factors responsible for this include dissatisfaction with the services provided. We assessed clients’ satisfaction with preventative services for malaria during pregnancy delivered at antenatal clinics (ANC) in Anambra State Nigeria.Method: We conducted a cross-sectional study among 284 pregnant women attending ANC using a three-stage sampling technique. Pre-tested semi-structured interviewer-administered questionnaire was used to collect information on socio-demographics, knowledge of malaria in pregnancy services and satisfaction with services. Responses to questions on satisfaction was on a 5-point Likert scale. A cut off of ≥75% of composite score was used to classify respondents as satisfied. For knowledge, every correct answer was scored 1 and incorrect 0; ≥75% of the composite score was graded as good knowledge. Chi square and logistic regression were used to test for association between client satisfaction and independent variables.Results: The mean age of participants is 28 years±4.4 years. Overall 62.2% were satisfied with quality of preventive services for malaria during pregnancy. However, 64.8% and 57.8% were dissatisfied with cost of healthcare and interpersonal relationship with the health workers (HWs). Majority of the of respondents (88.73%) had poor knowledge of malaria preventive services during pregnancy. Type of facility (Adjusted odds ratio [aOR] = 2.11; 95%CI: 1.20-3.71) and knowledge (aOR: 0.41; 95%CI: 0.18 – 0.90) were independently associated with satisfaction with interpersonal relationship respectively. Type of facility (aOR: 0.47; 95%CI: 0.27-0.80) and employment status (aOR: 3.07; 95%CI: 1.39 -6.74) were also independently associated with satisfaction with cost of healthcare.Conclusion: A fair proportion of respondents were satisfied with the preventive services for malaria during pregnancy provided even though most were dissatisfied with the cost of assessing care and interpersonal relationship with health workers. Uninterrupted availability of LLIN and IPT at all health facilities, subsidized cost of malaria-related commodities, and incentives for good customer satisfaction ratings may remediate the described barriers to seeking preventative care for malaria during pregnancy.
CA724 predicts overall survival in locally advanced gastric cancer patients with neoadjuvant chemotherapy
Authors: Tong Yilin , Zhao Yan , Shan Zexing , Zhang Jianjun
Published: August 2020

Background: Serum tumor markers including AFU, AFP, CEA, CA199, CA125 and CA724, are of great importance in the diagnosis, prognostic prediction and recurrence monitoring of gastrointestinal malignancies. However, their significance in gastric cancer (GC) patients with neoadjuvant therapy (NCT) is still uncertain. The aim of this study was to evaluate the predictive value of these six tumor markers in locally advanced GC patients who underwent NCT and curative surgery. Methods: In total, 290 locally advanced GC patients who underwent NCT and D2 radical gastrectomy were retrospectively analyzed. Data on their tumor markers before (pre-) and after (post-) NCT and pathological characteristics were extracted from the database of our hospital. The optimal cutoff values of the six tumor markers were calculated by the ROC curve and Youden index. Their predictive significance was analyzed and survival curves for overall survival (OS) were obtained by the Kaplan-Meier method. Associations between categorical variables were explored by the chi-square test or Fisher's exact test. Multivariate analyses were performed by the Cox regression model. Results: Pre- and post-CA199, -CA125 and -CA724 could predict overall survival (all P < 0.05), but only the change (diff-) of CA199 was related to prognosis (P = 0.05). In the multivariable analysis, pre- (P = 0.014) and post-CA724 (P = 0.036) remained significant, though diff-CA724 was not an independent prognostic factor (P = 0.581). In addition, pre- and post-CA199, -CA125 and -CA724 were associated with lymph node metastasis (N- vs N+) and pathological stage (Ⅰ-Ⅱ vs Ⅲ) (all P < 0.05). Moreover, post-CA724 was related to the vascular or lymphatic invasion (P = 0.019), while pre-CA724 was not (P = 0.082). However, AFU, AFP and CEA showed no association with survival (P > 0.05). Conclusions: CA724 is an independent factor for prognosis and could be used to predict ypN and ypTNM stage in locally advanced GC patients undergoing NCT and curative resection.
Challenges in health service delivery under public-private partnership in Tanzania: Stakeholders’ views from Dar es Salaam Region
Authors: Nuhu Said , Mpambije Chakupewa Joseph , Ngussa Kinamhala
Published: August 2020

Background: Public-private partnership in the health sector was introduced to improve the delivery of health services in Tanzania. Contrary, the expected outcomes have not been fully realised. This study aimed at investigating challenges encountered in implementing public-private partnership institutional arrangements in health service delivery in Kinondoni Municipality, Dar es Salaam, Tanzania.Methods: A qualitative case study design was employed, where in-depth interviews with stakeholders were held and document reviews conducted. Fourteen (n=14) participants engaged in this study. Eight (n=8) and six (n=6) of the fourteen participants were from the public and private sector, respectively. The thematic approach was used to analyse data, and ethical principles in the research process were upheld.Results: Findings revealed that although public-private partnerships are hailed for supplementing the government’s efforts in the provision of health services, institutional arrangements for the smooth provision of these services are lacking. Several challenges encumber smooth provision of health services and these include inadequate resources, ineffective monitoring and evaluation, and insufficient consultations between partners.Conclusion: Inadequate legal and policy framework, or ineffective implementation practices may influence challenges facing institutional arrangements for public-private partnerships. Therefore, strengthening of public-private partnerships is recommended to improve implementation mechanisms and practices such as adherence to partnership agreements and compliance to the policies, laws and regulations.
Effect of Resilience on Infertile Couples’ Quality of Life: An Actor–Partner Interdependence Model Approach
Authors: Ha Ju-Young , Ban Seonhwa
Published: August 2020

Background Infertility is an emerging socioeconomic issue affecting an individual’s life and the nation. However, only few studies have examined the influence of resilience on the quality of life (QoL) of spouses as actors and partners. Hence, this study aimed to analyze the influence of resilience, a positive factor that infertile couples may have, on QoL using the actor–partner interdependence model (APIM).Methods By the analyses of couples’ data, we analyzed the effect of resilience on the QoL of infertile couples as actors and partners. This cross-sectional study included 150 infertile couples. The Fertility Quality of Life and Resilience Scale was used to measure couples’ QoL and resilience. APIM was applied to analyze QoL.Results In terms of actor effects, the resilience of both wives (β = 0.201, p < 0.001) and husbands (β = 0.713, p < 0.001) had a significant effect on individual QoL. With regard to partner effects, husbands’ resilience (β = 0.351, p < 0.001) had a significant impact on wives’ QoL and the wives’ resilience (β = 0.219, p = 0.009) had a significant impact on husbands’ QoL.Conclusions The resilience of an infertile actor was found to affect both his/her own QoL and his/her partner’s QoL. In the future, if a program is to be developed to improve couples’ QoL, both spouses should work together to improve their resilience, thereby improving their QoL.
An investigation of an anthrax outbreak in Makoni District, Zimbabwe
Authors: Makurumidze Richard , Tafara Gombe Notion , Tapuwa Magure , Tshimanga Mufuta
Published: August 2020

Background: In Zimbabwe, anthrax continues to be a disease of public health importance with sporadic outbreaks reported in many parts of the country annually. A human anthrax outbreak occurred in Makoni District Ward 22 and 23 between June 2013 and February 2014. The human anthrax outbreak followed cattle deaths in the wards, which were laboratory confirmed to be due to anthrax. We report the clinical characteristics, distribution of anthrax cases (places, person and time), risk factors for contracting the disease, environmental assessment, district preparedness and response and outbreak prevention and control measures. Methods: We conducted an outbreak investigation with the design of a 1:1 case-control study. Cases and controls were frequency matched against sex. Data were collected using a structured interviewer-administered questionnaire. Environmental assessment, district preparedness and response and outbreak prevention and control measures were assessed using a checklist, observations and key informant interviews. Multivariable unconditional logic regression analysis was performed to identify independent risk factors associated with contracting anthrax. Results: Of the 64 cases, 37 cases and 37 controls were interviewed. All the cases had cutaneous anthrax with the commonest site of eschar being the hand (43%). Most of the cases (89%) were managed according to the national guidelines. On multivariable analysis, source of meat from other villagers [vs butchery, OR = 15.21, 95% CI (2.32-99.81)], skinning [OR = 4.32, 95% CI (1.25-14.94)] and belonging to a religion which permits eating meat from cattle slaughtered due to unknown illness or butchered after an unobserved death [OR = 6.12, 95% CI (1.28-29.37)] were associated with contracting anthrax. The district was poorly resourced and delayed to respond to the outbreak. Conclusion: The described anthrax outbreak resulted from contact and consumption of infected cattle meat. The district office response was delayed and was not prepared to control the outbreak. However, the outbreak was eventually controlled through cattle vaccination; health education and awareness campaigns. The district should strengthen its emergency preparedness and response capacity, revive zoonotic committees, conduct awareness campaign during the high-risk period and improve the surveillance of anthrax during high-risk periods.
A participatory surveillance of marsh deer (Blastocerus dichotomus) morbidity and mortality in Argentina: first results.
Authors: Orozco Maria Marcela , Argibay Hernán D. , Minatel Leonardo , Guillemi Eliana C. , Berra Yanina , Schapira Andrea , Nucci Dante Di , Marcos Andrea , Lois Fernanda , Falzone Martin , Farber Marisa D.
Published: August 2020

Background: In an era of unprecedented socio-ecological changes, managing wildlife health demands high-quality data collection and the engagement of local communities. Blastocerus dichotomus, the largest South American deer, is Vulnerable to extinction mainly due to habitat loss. Diseases have been recognised as a potential threat, and winter mortality has been historically described in marsh deer populations from Argentina. Field difficulties have, however, prevented in-depth studies of their health status.Results: Between May 2014 and April 2017, we investigated marsh deer morbidity and mortality in the two largest populations in Argentina. We collected data by means of a passive surveillance system that involved a network of researchers, field partners (veterinarians, park rangers, and local community), and decision makers. We sampled dead deer during as well as outside mortality events. A total of 44 marsh deer with different body condition scores were evaluated. We obtained haematology and biochemistry values from animals with good body condition score. Marsh deer with poor body condition had a high burden of the ticks Amblyomma triste and Rhipicephalus microplus. Vector-borne agents such as Theileria cervi, Trypanosoma theileri, Trypanosoma evansi, Ehrlichia chaffeensis, Anaplasma platys, Anaplasma odocoilei, Anaplasma marginale, and Candidatus Anaplasma boleense were also found. Haemonchus spp., Ostertagia spp., and Trichostrongylus spp. were the most frequent gastrointestinal parasites in deer with poor body condition. A Multiple Correspondence Analysis reinforced a possible association of winter period with lower body score condition, high tick loads, infection with E. chaffeensis, and presence of harmful gastrointestinal parasites. Conclusions: Our approach allowed the establishment of a participatory surveillance network of marsh deer morbidity and mortality in Argentina. We report and analyse the first data obtained opportunistically within the framework of this network, providing information on the infectious and parasitic agents in marsh deer populations. The occurrence of Fasciola hepatica and Leptospira interrogans serovar pyrogenes is reported for the first time in wild marsh deer from Argentina. Our data will be useful to improve the interpretation of future mortality events. The field implementation of a surveillance network is key to a holistic approach to wildlife diseases.
The impact of patient safety culture and the leader coaching behaviour of nurses on the intention to report errors: A cross-sectional survey
Authors: Chegini Zahra , Kakemam Edris , Jafarabadi Mohammad Asghari , Janati Ali
Published: August 2020

Background: There is growing interest in examining the factors affecting the reporting of errors by nurses. However, little research has been conducted into the effects of perceived patient safety culture and leader coaching of nurses on the intention to report errors.Methods: This cross-sectional study was conducted amongst 256 nurses in the emergency departments of 18 public and private hospitals in Tabriz, northwest Iran. Participants completed the Hospital Survey on Patient Safety Culture (HSOPSC), Coaching Behavior Scale and Intention to Report Errors’ questionnaires and the data was analyzed using multiple linear regression analysis.Results: Overall, 43% of nurses had an intention to report errors; 50% of respondents reported that their nursing managers demonstrated high levels of coaching. With regard to patient safety culture, areas of strength and weakness were “teamwork within units” (PRR = 66.8%) and “non-punitive response errors” (PRR = 19.7%). Regression analysis findings highlighted a significant association between an intention to report errors and patient safety culture (B = 0.2, CI 95%: 0.1 to 0.3, P < 0.05), leader coaching behavior (B = 0.2, CI 95%: 0.1 to 0.3, P < 0.01) and nurses’ educational status (B = 0. 8, 95% CI: -0.1 to 1.6, P < 0.05).Conclusions: Further research is needed to assess how interventions addressing patient safety culture and leader coaching behaviours might increase the intention to report errors.
Feeding practices in 6-24-month-old children with chronic cholestatic liver diseases: A mixed-method study
Authors: Chen Xiao , Wang Jianshe , Lu Yi , Xie Xinbao , Gu Ying , Latour Jos M. , Zhang Yuxia
Published: August 2020

Background: Children with chronic cholestatic liver diseases have a high risk of malnutrition. However, nutritional management in China has received little attention, and there has been limited evidence regarding improving these practices. This study aimed to evaluate the feeding status of chronic cholestatic children aged 6-24 months and to explore their parents’ experiences with feeding practices. Methods: A mixed-method study was conducted among parents of 74 children with chronic cholestatic liver diseases. The Infant and Young Child Feeding Index (ICFI) was used to collect quantitative data of feeding practices. In-depth interviews with parents were performed to generate qualitative data. Multivariate analysis was conducted to identify predictors of inappropriate feeding practices. Qualitative data were analyzed using thematic analysis.Results: Only 16.2% of the children had appropriate feeding practices. In terms of dairy products, the rate of breastfeeding, medium-chain triglyceride formula feeding, and regular formula feeding were 25.7%, 39.2% and 44.6% respectively. The complementary feeding rate was 68.8%, and the age of adding complementary foods was 6.9±1.7 months. Consumption of foods from animal sources was suboptimal, 66.7% of the children aged 6-8 months and 45.5% of the children aged 9-11 months had carbohydrates as a single source of complementary foods and had no intake of meat, eggs or bean products, while in the age group 12-24 months, 52.0% of the children had eggs less than 2 days/week, 60.0% had meat less than 2 days/week, and 60.0% had no intake of bean products. Low literacy of the primary care provider was significantly related to inappropriate feeding practices (adjusted OR 5.52; 95% CI 1.29, 23.66). The result of the interviews indicated that parents generally lacked the scientific knowledge of feeding and thought that the intake of animal source foods and oils would be a burden to the liver and cause damage to the liver functions.Conclusion: Parents generally lacked science-based feeding knowledge and the feeding practices in 6-24-month-old children diagnosed with chronic cholestatic liver diseases fell short of the recommendations in current nutritional guidelines. Appropriate interventions targeting social and cultural family circumstances should therefore be included in supporting parents in feeding practices to improve children’s clinical outcomes.
Proteomic and metabolic profile analysis of low-temperature storage responses in Ipomoea batata Lam. tuberous roots
Authors: Cui Peng , Li Yongxin , Cui Chenke , Huo Yanrong , Lu Guoquan , Yang Huqing
Published: August 2020

Background: Sweetpotato (Ipomoea batatas L.) is one of the seven major food crops grown worldwide, with 70-75% of production in China. Cold stress often can cause protein expression pattern and substance contents variations for tuberous roots of sweetpotato during low-temperature storage. Recently, we developed proteometabolic profiles of the fresh sweetpotatoes (cv. Xinxiang) in an attempt to discern the cold stress-responsive mechanism of tuberous root crops during post-harvest storage.Results: For roots stored under 4℃ condition, the CI index, REC and MDA content in roots were significantly higher than them at control temperature (13℃). The activities of SOD, CAT, APX, O2.- producing rate, proline and especially soluble sugar contents were also significantly increased. Most of the differentially expressed proteins (DEPs) were implicated in pathways related to metabolic pathway (~22%), especially phenylpropanoids (~10%) and followed by starch and sucrose metabolism (~3%). Proteins including L-ascorbate peroxidase 3 and catalase were down-regulated during low temperature (4℃) storage. α-amylase, sucrose synthase and fructokinase were significantly up-regulated in starch and sucrose metabolism, while β-glucosidase, glucose-1-phosphate adenylyl-transferase and starch synthase were opposite. Furthermore, metabolome profiling revealed that glucosinolate biosynthesis, tropane, piperidine and pyridine alkaloid biosynthesis as well as protein digestion and absorption played a leading role in metabolic pathways of sweetpotato roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis. Data are available via ProteomeXchange with identifier PXD017728.Conclusions: Our proteomic and metabolic profile analysis of sweetpotato roots stored at low temperature reveal that the antioxidant enzymes activities, proline and especially soluble sugar content were significantly increased. Most of the DEPs were implicated in phenylpropanoids and followed by starch and sucrose metabolism. The discrepancy between proteomic (L-ascorbate peroxidase 3 and catalase) and biochemical (APX/CAT enzyme activity) data may be explained by higher H2O2 levels and increased glutathione and ascorbate redox states, which enhanced the CAT/APX enzyme activity indirectly. Glucosinolate biosynthesis played a leading role in metabolic pathways of sweetpotao roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis.
How does the self-reported health of undergraduate nursing students change during their degree programme? Survey results from a Scottish University
Authors: Evans Josie , Andreis Federico , Cameron Dawn M , Eades Claire E
Published: August 2020

Background: The lifestyle behaviours, and the physical and mental health of nurses, are poorer than those of other allied health professionals, and of the general population. However, these were no less favourable among first year undergraduate nursing students at a Scottish Higher Education Institution (HEI) than among similar people of the same age. We compared health and health behaviours among the same cohort of undergraduate nursing students over the course of their degree. Methods: An anonymous self-complete repeat cross-sectional survey was administered during a timetabled teaching session at three time-points to undergraduate nursing students at the start of Years 1, 2 and 3 of their programme. They had received written information about the study previously. Results: Self-reported health did not change significantly over time, but there was a clear decline over the three years in the proportions of students rating their mental health as excellent/very good/good and a concomitant increase in those rating their mental health as fair/poor. Correspondingly, the mean WEMWBS wellbeing score declined over the three years, with the proportion of students with a score of <46 (indicating either high risk of major depression, or in high risk of psychological distress and increased risk of depression) increasing from one quarter to one half. This effect was captured and described using a Bayesian regression analysis. The most noticeable change in health behaviours was a decline in physical activity levels over the study period. The proportion of students managing 150 minutes of weekly physical activity decreased from three quarters to two thirds. This was reflected in higher self-reported sedentary time, although there were no observable trends over time in mean BMI, or proportions of students categorised as overweight or obese. Conclusions: This paper suggests that there may be a decline in mental health and in participation in physical activity among nursing students over the course of their degree. We recommend the incorporation of an intervention into the undergraduate nursing curriculum that promotes and encourages regular physical activity, offering students the opportunity to learn about health promotion and lifestyle change in practice, to improve their own physical health, and to address mental wellbeing.
Prevalence and Correlates of Mycoplasma genitalium Infections among Patients Attending a Sexually Transmitted Infection Clinic in Guangdong, China a Cross-sectional Study
Authors: Zhang Xiaohui , Zhao PZ , Ke WJ , Wang LY , Tso Lai Sze , Chen ZY , Liao YY , Liang CM , Chen HR , Ren XQ , Huang JM , Ong JJ , Yang F , Yang Ligang
Published: August 2020

Background: Mycoplasma genitalium (MG) causes urogenital tract infections and is associated with reproductive morbidity. Although MG has been reported across many regions and population groups, it is not yet routinely tested for in China. Our study contributes to current research by reporting the prevalence and correlates of MG infection in patients attending a sexually transmitted infection (STI) clinic in Guangdong from Jan 2017-May 2018.Methods: Urethral (from 489 men) and endo-cervical (from 189 women) samples, blood samples, and patient histories (via questionnaires) were collected. Doctors clinically diagnosed anogenital warts (GW) during the examination (n=678). The presence of MG was evaluated using an in-house via polymerase chain reaction protocol. We also tested all participants for herpes simplex virus-2 (HSV-2), Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), syphilis and HIV. Univariate and multivariate logistic regression were used to evaluate factors associated with MG.Results: MG was detected in 7.2% (49/678) of the patients (men, 7.4%; women, 6.9%). The MG positivity rate was 14.2% among symptomatic patients, and 5.6% for asymptomatic patients , respectively. Only 36.7% (18/49) Mg positive patients were symptomatic. Among the MG-infected patients, 10.2% were co-infected with CT, 6.1% with NG, 8.2% with HSV-2, 4.1% with syphilis and 22.4% with GW. Presentation with clinical symptoms was significantly associated with MG infection [OR=2.52 (2.03-3.13)]. In our analysis, MG was not associated with other STIs.Conclusions: MG is a relatively common infection among individuals attending an STI clinic in Guangdong Province. Routine testing of symptomatic patients may be necessary, and more epidemiological studies are needed to provide evidence for future testing guidelines,
Dorsal approach with Glissonian approach in laparoscopic anatomic hepatectomy for right lobe
Authors: Wang Shaohe , Yue Yang , Zhang Wenjie , Liu Qiaoyu , Sun Beicheng , Sun Xitai , Yu Decai
Published: August 2020

Background

Laparoscopic anatomic hepatectomy (LAH) has gradually become a routine surgical procedure. We previously reported that Glissonian approach combined with major hepatic vein first was effective for LAH. Because dorsal approach could effectively expose the major hepatic vein, we merged it with Glissonian approach in LAH for the right lobe.

Methods

Twenty patients who underwent LAH from January 2017 to November 2018 were retrospectively analysed. Of these patients, seven patients underwent laparoscopic right hemihepatectomy (LRH group), seven patients who underwent laparoscopic right posterior hepatectomy (LRPH group), and six patients who underwent laparoscopic hepatectomy for segment 7 (LS7 group). First, the paracaval portion of caudate lobe along the IVC was transected through dorsal approch after the corresponding hepatic pedicles were isolated through Glissonian approach. Next, the liver parenchyma was transected by dorsal approach until the corresponding major hepatic vein was exposed from its trunk to the root. Then, the liver parenchyma was transected by a ventral approach. Finally, the root of the major hepatic vein was transected.

Results

The mean age of the patients was 53.8 years and the male: female ratio was 8:12. The median operation time was 306.0 ± 58.2 min and the mean estimated volume of blood loss was 412.5 ± 255.4 mL. The mean duration of postoperative hospital stay was 10.2 days. The mean Pringle maneuver time was 64.8 ± 27.7 min. Five patients received transfusion of 2–4 U of red blood cells. Two patients suffered from transient hepatic dysfunction and one suffered from pleural effusion. None of the patients underwent conversion to an open procedure. The operative duration, volume of the blood loss, Pringle maneuver time, and postoperative hospital stay duration did not differ significantly among the LRH, LRPH, and LS7 groups (P > 0.05).

Conclusion

Dorsal approach combined with Glissonian approach for right lobe in LAH is feasible and effective, although it is essential to include more cases for further study.
Antimicrobial resistance among GLASS pathogens in conflict and non-conflict affected settings in the Middle East: A systematic review
Authors: Truppa Claudia , Abo-Shehada Mahmoud N.
Published: August 2020

Background: In spite of the evident general negative effects of armed conflict on countries’ health systems and populations’ health outcomes, little is known about similar impacts of conflicts on the spread of antimicrobial resistances (AMR). This review was to address this evidence gap and describe:1. Patterns of AMR in the Middle East (ME) and resistance profiles of pathogens included in the Global AMR Surveillance System (GLASS) supported by the World Health Organization;2. Differences in proportions of AMR isolates between conflict and non-conflict countries. Methods: A systematic literature review was conducted following PRISMA guidelines and searching five electronic databases. Subject heading and free text were searched for “antimicrobial resistances” and “Middle East”, to identify observational studies on AMR published from January 2011 to June 2018. Data were extracted from included articles on a predefined set of variables. Percentages of AMR were analysed as median and interquartile ranges. Risk of bias was assessed using the Newcastle-Ottawa Scale. Results: A total of 132 articles met the inclusion criteria. Included studies showed heterogeneity in study design, laboratory methods and standards for interpretation of results, and an overall high risk of bias. Main findings were the following:1. High proportions of carbapenem resistance in Acinetobacter spp. (median 74.2%: range), and both carbapenem resistance (median 8.1% and 15.4% for E. coli and K. pneumoniae respectively) and ESBL-production (median 32.3% and 27.9% for E. coli and K. pneumoniae respectively) amongst Enterobacteriaceae. S. aureus isolates showed a median methicillin resistance percentage of 45.1%, while vancomycin resistance was almost absent. A median of 50% of the strains of S. pneumoniae showed non-susceptibility to penicillin. 2. Similar trends were observed in conflict and non-conflict affected countries.Conclusions: There is a lack of standardization in the methodological approach to AMR research in the Middle East. The proportion of antibiotic resistances among specific GLASS pathogens is high, particularly among Acinetobacter spp.
Correlates of knowledge of family planning among people living in fishing communities of Lake Victoria, Uganda
Authors: Nanvubya Annet , Wanyenze R.K. , Nakaweesa T. , Mpendo J. , Kawoozo B. , Matovu F. , Nabukalu S. , Omoding G. , Kaweesi J. , Ndugga J. , Kamacooko O. , Chinyenze K. , Price M. A. , Geertruyden J.P. Van
Published: August 2020

BackgroundKnowledge of family planning (FP) is a key determinant of contraceptive use which ultimately plays a role in attainment of good health and in conduct of clinical research. People living in fishing communities (FCs) have limited access to health services including FP and are targeted for future clinical research but their knowledge of FP and its correlates are scantily known. We determined correlates of knowledge of FP among people living in FCs of L. Victoria in Uganda to inform future FP education programs in FCs. MethodsWe conducted a comparative cross-sectional survey among participants aged 15-49 years from Kigungu and Nsazi. Participants were asked if they were aware of any FP method. All those who responded in the affirmative were further asked to mention what FP methods they had heard of or knew. Those who reported knowledge of at least one FP method were asked a series of questions about FP methods and their side effects. Knowledge was categorized into good or poor knowledge based on their mean total score. Poor knowledge constituted a score below the mean while good knowledge constituted a score of more than or equal to the mean total score. To further explore attitudes and perceptions of FP, ten in-depth interviews and four focus group discussions were conducted. ResultsOf the 1,410 screened participants, 94.5% were aware of at least one FP method. Pills and injectable hormonal methods were the most commonly known methods. Slightly over a third (38%) had good knowledge of FP. Correlates of knowledge of FP were; being female (aOR: 1.92 95% CI: 1.39-2.67), residing in Kigungu (aOR: 4.01 95% CI: 2.77-5.81), being married (aOR: 1.59 95% CI: 1.11-2.28) and currently being in a sexual relationship (aOR: 1.75 95% CI: 1.18-2.60). Concerns about safety and effectiveness of some modern FP methods exist. Misconceptions on effects of FP like sterility, cancers and foetal abnormalities were common. ConclusionFP awareness among people living in FCs of L. Victoria in Uganda is high. However, good knowledge about specific methods tends to be low. Correlates of knowledge of FP include gender, residence, marital status and sexual engagement.
Majocchi’s Granuloma on the Forearm Caused by Trichophyton tonsurans in an Immunocompetent Patient
Authors: Zheng Yun-yan , Li Yue , Chen Ming-yan , Mei Qian-yun , Zhang Ru-zhi
Published: August 2020

Majocchi's granuloma is an uncommon fungal infection of the dermis and subcutaneous tissue. The most frequently identified cause of Majocchi’s granuloma is anthropophilic Trichophyton rubrum, and it is most commonly located on the anterior aspect of the lower limbs in women. Here, we report a case of Majocchi’s granuloma on the forearm, a site that is rarely involved, in a 62-year-old woman who had been bitten by a dog. Histological examination revealed a dense dermal infiltrate composed of lymphoplasmacytic cells and neutrophils, with hyphae in the dermis. The presence of the fungus, Trichophyton tonsurans, was confirmed by mycological examination and molecular methods. Therefore, histological and mycological examination confirmed the diagnosis of Majocchi’s granuloma. The patient was treated with local moxibustion and itraconazole, 200 mg/day, for 60 days, which facilitated a complete resolution of the lesions.