bluuebunny/crossref_metadata_2025 huggingface dataset:
Thoracic Aortic Calcification and Pre-Clinical Hypertension by New 2017 ACC/AHA Hypertension Guidelines
Authors: Jan Ya-Ting , Tsai Pei-Shan , Longenecker Chris T. , Lin Dao-Chen , Yun Chun-Ho , Sung Kuo-Tzu , Liu Chuan-Chuan , Kuo Jen-Yuan , Hung Chung-Lieh , Wu Tung-Hsin , Lin Jiun-Lu , Hou Charles Jia-Yin , Tsai Cheng-Ting , Chien Chen-Yen , So Aaron
Published: June 2021

The recently revised 2017 American College of Cardiology/American Heart Association (ACC/AHA) hypertension (HTN) guidelines employ a lower blood pressure threshold to define HTN, aiming for earlier prevention of HTN-related cardiovascular diseases (CVD). Thoracic aortic calcification (TAC), a new surrogate marker of aging and aortic medial layer degeneration, and different stages of HTN, according to the 2017 ACC/AHA HTN guidelines, remain unknown. We classified 3022 consecutive asymptomatic individuals enrolled into four HTN categories using the revised 2017 ACC/AHA guidelines: normal blood pressure (NBP), elevated blood pressure (EBP), and stage 1 (S1) and stage 2 (S2) HTN. The coronary artery calcification score and TAC metrics (total Agaston TAC score, total plaque volume (mm3), and mean density (Hounsfield units, HU)) were measured using multi-detector computed tomography. Compared to NBP, a graded and significant increase in the TAC metrics was observed starting from EBP and S1 and S2 HTN, using the new 2017 ACC/AHA guidelines (NBP as reference; all trends: p < 0.001). These differences remained consistent after being fully adjusted. Older age (>50 years), S1 and S2 HTN, prevalent diabetes, and chronic kidney disease (<60 mL/min/1.73 m2) are all independently contributing factors to higher TAC risk using multivariate stepwise logistic regressions (all p ≤ 0.001). The optimal cutoff values of systolic blood pressure, diastolic blood pressure, and pulse pressure were 121, 74, and 45 mmHg, respectively, for the presence of TAC after excluding subjects with known CVD and ongoing HTN medication treatment. Our data showed that the presence of TAC starts at a stage of elevated blood pressure not categorized as HTN from the updated 2017 ACC/AHA hypertension guidelines.
Clinical Application of Dynamic SPECT/CT in a Patient with Prior Myocardial Infarction Underwent Percutaneous Coronary Intervention Twice
Authors: Chan Hung-Pin , Hu Chin , Yang Ming-Hui , Chan Hung-Yen , Peng Nan-Jing
Published: June 2021

We present a case of CAD with anteroseptal MI after stent insertion for revascularization due to symptoms presented. MPI with dynamic SPECT/CT provided useful information in terms of flow parameters and matched territories of stenting results as well as providing coronary artery flow phenomenon underwent PCI. In this case, dynamic SPECT/CT may minimize errors with proper stents treatment, especially for controversial MPI results.
Assessing the Economic and Environmental Impacts of Alternative Renewable Portfolio Standards: Winners and Losers
Authors: Mamkhezri Jamal , Malczynski Leonard A. , Chermak Janie M.
Published: June 2021

State-mandated renewable portfolio standards affect substantial portions of the total U.S. electricity supply. Renewable portfolio standards are environmentally motivated policies, yet they have the potential to greatly impact economy. There is not an agreement in the literature on the impact of renewable portfolio standards policies on regional economies, especially on job creation. By integrating various methodologies including econometrics, geographic information system, and input–output analysis into a unique system dynamics model, this paper estimates the economic and environmental impacts of various renewable portfolio standards scenarios in the state of New Mexico, located in Southwestern U.S. The state is endowed with traditional fossil fuel resources and substantial renewable energy potential. In this work we estimated and compared the economic and environmental tradeoffs at the county level under three renewable portfolio standards: New Mexico’s original standard of 20% renewables, the recently adopted 100% renewables standard, and a reduced renewable standard of 10%. The final one would be a return to a more traditional generation profile. We found that while the 20% standard has the highest market-based economic impact on the state as a whole, it is not significantly different from other scenarios. However, when environmental impacts are included, the 100% standard yields the highest value. In addition, while the state level economic impacts across the three scenarios are not significantly different, the county-level impacts are substantial. This is especially important for a state like New Mexico, which has a high reliance on energy for economic development. A higher renewable portfolio standard appears to be an economic tool to stimulate targeted areas’ economic growth. These results have policy implications.
Carbon Capture, Utilisation and Storage as a Defense Tool against Climate Change: Current Developments in West Macedonia (Greece)
Authors: Koukouzas Nikolaos , Tyrologou Pavlos , Karapanos Dimitris , Carneiro Júlio , Pereira Pedro , de Mesquita Lobo Veloso Fernanda , Koutsovitis Petros , Karkalis Christos , Manoukian Eleonora , Karametou Rania
Published: June 2021

In West Macedonia (Greece), CO2 accounts as one of the largest contributors of greenhouse gas emissions related to the activity of the regional coal power plants located in Ptolemaida. The necessity to mitigate CO2 emissions to prevent climate change under the Paris Agreement’s framework remains an ongoing and demanding challenge. It requires implementing crucial environmentally sustainable technologies to provide balanced solutions between the short-term needs for dependency on fossil fuels and the requirements to move towards the energy transition era. The challenge to utilise and store CO2 emissions will require actions aiming to contribute to a Europe-wide CCUS infrastructure. The Horizon 2020 European Project “STRATEGY CCUS “examines the potential for CO2 storage in the Mesohellenic Trough from past available data deploying the USDOE methodology. Research results show that CO2 storage capacities for the Pentalofos and Eptachori geological formations of the Mesohellenic Trough are estimated at 1.02 and 0.13 Gt, respectively, thus providing the potential for the implementation of a promising method for reducing CO2 emissions in Greece. A certain storage potential also applies to the Grevena sub-basin, offering the opportunity to store any captured CO2 in the area, including other remote regions.
Method of Limiting Shaft Voltages in AC Electric Machines
Authors: Berhausen Sebastian , Jarek Tomasz
Published: June 2021

The article presents a new method of counteracting shaft voltages and currents in AC electrical machines. It is based on the use of an auxiliary winding located in the stator of the machine. The design of a test stand adapted to the measurement of shaft voltages of the machine, based on the prototype of a synchronous machine with permanent magnets, has been presented. The model was used to conduct a number of laboratory tests aimed at confirming the functionality of the auxiliary winding in various operating states of the machine (including no-load and load condition during generator operation). The article focuses on demonstrating the beneficial effect of the auxiliary winding on the level of induced shaft voltages in an electric machine. In order to confirm the close dependence of the circular flux in the stator yoke on the shaft voltage, shaft voltage measurement results for various cases of external power supply of auxiliary winding forcing a circular flux are presented. Regardless of the laboratory tests, a simulation model of a synchronous machine with permanent magnets, on which calculations were carried out to analyze the work of the auxiliary winding located in the stator yoke, was developed. The article is supplemented by a review of damage to electrical machines with a detailed description of bearing defects, as well as a brief de-scription of issues related to the mechanism of generating shaft voltages and currents in electrical machines and methods of counteracting them.
Electrical and Mechanical Characteristics of a High-Speed Motor for Electric Turbochargers in Relation to Eccentricity
Authors: Lee Tae-Woo , Hong Do-Kwan
Published: June 2021

As the demand for eco-friendly, high-efficiency transportation technologies increase due to climate change, a high-speed electric motor, a key component of an electric turbocharger, has been developed that can reduce emissions and increase fuel efficiency. Korea Electrotechnology Research Institute with Keyyang Precision Co., Ltd., developed a high-speed surface-mounted permanent magnet synchronous motor. It operates at a power of 3 kW at 100,000 rpm and is intended to fit 1600 cc diesel vehicles. In this paper, the electrical and mechanical characteristics of the high-speed motor were reviewed in consideration of the effect of eccentricity among the various causes that affect vibration. It was confirmed that eccentricity affected the distribution of the electromagnetic force and inductance of the winding due to the uneven air-gap. Additional vibration was generated at the half of pole passing frequency (1666.67 Hz). Diagnosing the presence or absence of eccentricity when driving a motor takes a great deal of time and cost because the load is separated or the motor is diagnosed through disassembly and measurement. The characteristics of eccentricity identified in this paper can be checked using a relatively simple method when diagnosing the presence or absence of actual eccentricity.
Particulate Matter Reduction in Residual Biomass Combustion
Authors: Nugraha Maulana G. , Saptoadi Harwin , Hidayat Muslikhin , Andersson Bengt , Andersson Ronnie
Published: June 2021

Counteracting emissions of particulate matter (PM) is an increasingly important goal in sustainable biomass combustion. This work includes a novel approach to investigate the PM emissions, originating from residual biomass combustion, at different combustion conditions in a lab-scale grate-fired furnace and includes in situ PM measurements by using on-line sensors. The interior furnace design allows installation of baffles to suppress the emissions by controlling the residence time. Moreover, the two-thermocouple method is used to measure the true gas temperature, and an on-line spatially resolved PM measurement method is developed to study the evolution of the PM concentration throughout the furnace for different experimental conditions thereby allowing accurate in-situ measurement of the PM reactivity. Experimental results and computational fluid dynamics (CFD) analyses are utilized in the current work to develop a kinetic model for reduction of particulate matter emissions in biomass combustion. The discrete particle model (DPM) is utilized in CFD analysis to improve the understanding of the particle temperature and residence time distribution which are difficult to quantify experimentally. By combining the experimental measurements of real soot formed during biomass combustion and information from the CFD analyses, a predictive kinetic model for PM10 reduction in biomass combustion is successfully developed.
Unified Power Converter Based on a Dual-Stator Permanent Magnet Synchronous Machine for Motor Drive and Battery Charging of Electric Vehicles
Authors: Pedrosa Delfim , Monteiro Vitor , Sousa Tiago J. C. , Machado Luis , Afonso Joao L.
Published: June 2021

An electric vehicle (EV) usually has two main power converters, namely one for the motor drive system and another for the battery-charging system. Considering the similarities between both converters, a new unified power converter for motor drive and battery charging of EVs is propounded in this paper. By using a single unified power converter, the cost, volume, and weight of the power electronics are reduced, thus also making possible a reduction in the final price of the EV. Moreover, the proposed unified power converter has the capability of bidirectional power flow. During operation in traction mode, the unified power converter controls motor driving and regenerative braking. Additionally, during operation in battery-charging mode, with the EV plugged into the electrical power grid, the unified power converter controls the power flow for slow or fast battery charging (grid-to-vehicle (G2V) mode), or for discharging of the batteries (vehicle-to-grid (V2G) mode). Specifically, this paper presents computer simulations and experimental validations for operation in both motor-driving and slow battery-charging mode (in G2V and V2G modes). It is demonstrated that the field-oriented control used in the traction system presents good performance for different values of mechanical load and that the battery-charging system operates with high levels of power quality, both in G2V and in V2G mode.
Performance Analysis of TiO2-Modified Co/MgAl2O4 Catalyst for Dry Reforming of Methane in a Fixed Bed Reactor for Syngas (H2, CO) Production
Authors: Mazhar Arslan , Khoja Asif Hussain , Azad Abul Kalam , Mushtaq Faisal , Naqvi Salman Raza , Shakir Sehar , Hassan Muhammad , Liaquat Rabia , Anwar Mustafa
Published: June 2021

Co/TiO2–MgAl2O4 was investigated in a fixed bed reactor for the dry reforming of methane (DRM) process. Co/TiO2–MgAl2O4 was prepared by modified co-precipitation, followed by the hydrothermal method. The active metal Co was loaded via the wetness impregnation method. The prepared catalyst was characterized by XRD, SEM, TGA, and FTIR. The performance of Co/TiO2–MgAl2O4 for the DRM process was investigated in a reactor with a temperature of 750 °C, a feed ratio (CO2/CH4) of 1, a catalyst loading of 0.5 g, and a feed flow rate of 20 mL min−1. The effect of support interaction with metal and the composite were studied for catalytic activity, the composite showing significantly improved results. Moreover, among the tested Co loadings, 5 wt% Co over the TiO2–MgAl2O4 composite shows the best catalytic performance. The 5%Co/TiO2–MgAl2O4 improved the CH4 and CO2 conversion by up to 70% and 80%, respectively, while the selectivity of H2 and CO improved to 43% and 46.5%, respectively. The achieved H2/CO ratio of 0.9 was due to the excess amount of CO produced because of the higher conversion rate of CO2 and the surface carbon reaction with oxygen species. Furthermore, in a time on stream (TOS) test, the catalyst exhibited 75 h of stability with significant catalytic activity. Catalyst potential lies in catalyst stability and performance results, thus encouraging the further investigation and use of the catalyst for the long-run DRM process.
Community-Based Business on Small Hydropower (SHP) in Rural Japan: A Case Study on a Community Owned SHP Model of Ohito Agricultural Cooperative
Authors: Alam Zafar , Watanabe Yoshinobu , Hanif Shazia , Sato Tatsuro , Fujimoto Tokihiko
Published: June 2021

Energy is the prerequisite for social and economic development of a community and country. In Japan, national government is promoting small hydropower (SHP) through a renewable energy policy by providing a high FIT price of 34 yen (≒0.32 US$/kWh) on energy generated from an SHP of less than 200 kW. Until now, the energy generation was controlled by national government agencies, but now independent power generation businesses are growing at the local community level in rural Japan. For the future growth of SHP, it is necessary to make electricity generation at the local community level. Therefore, these local communities will install and manage their renewable electricity by themselves. It will help to make the community self-sustainable and independent from the national government, and at the same time, it will also lead them to achieve the Sustainable Developments Goals (SDGs) target from community-based action. This paper aimed to discuss an SHP development business model in which local community will become the business owner of the SHP. It means “of the community, by the community and for the community”. The community identifies their renewable energy potential and needs, they borrow money from the financial organization or banks, install the power plant and do necessary maintenance and management by themselves. The revenue earned by selling electricity is used to repay the loan, and the rest is used for community development directly (such as local roads construction, agriculture land improvements, community hall maintenance, waterways maintenance, welfare, etc.). This paper also discussed a community-based 50 kW SHP installed in Miyazaki prefecture of Japan as a case study. This SHP is one of the best examples of a community ownership model (Community-based business model). A detailed explanation from planning to investment has been discussed. The local community is getting approximately 112,000 USD per year by selling the electricity, and 162-ton CO2 is estimated to decrease yearly, which will support the achievement of SDGs. Finally, installing this kind of SHP in remote areas will provide managerial skills to the local community directly, plant operation knowledge, and education to local students. Local communities learn the problem-solving skills, which lead them to solve the local problem on a community level by themselves.
Overview of Solutions for the Low-Temperature Operation of Domestic Hot-Water Systems with a Circulation Loop
Authors: Benakopoulos Theofanis , Vergo William , Tunzi Michele , Salenbien Robbe , Svendsen Svend
Published: June 2021

The operation of typical domestic hot water (DHW) systems with a storage tank and circulation loop, according to the regulations for hygiene and comfort, results in a significant heat demand at high operating temperatures that leads to high return temperatures to the district heating system. This article presents the potential for the low-temperature operation of new DHW solutions based on energy balance calculations and some tests in real buildings. The main results are three recommended solutions depending on combinations of the following three criteria: district heating supply temperature, relative circulation heat loss due to the use of hot water, and the existence of a low-temperature space heating system. The first solution, based on a heating power limitation in DHW tanks, with a safety functionality, may secure the required DHW temperature at all times, resulting in the limited heating power of the tank, extended reheating periods, and a DH return temperature of below 30 °C. The second solution, based on the redirection of the return flow from the DHW system to the low-temperature space heating system, can cool the return temperature to the level of the space heating system return temperature below 35 °C. The third solution, based on the use of a micro-booster heat pump system, can deliver circulation heat loss and result in a low return temperature below 35 °C. These solutions can help in the transition to low-temperature district heating.
Assessment of the Environmental Impact of Using Methane Fuels to Supply Internal Combustion Engines
Authors: Biernat Krzysztof , Samson-Bręk Izabela , Chłopek Zdzisław , Owczuk Marlena , Matuszewska Anna
Published: June 2021

This research paper studied the environmental impact of using methane fuels for supplying internal combustion engines. Methane fuel types and the methods of their use in internal combustion engines were systematized. The knowledge regarding the environmental impact of using methane fuels for supplying internal combustion engines was analyzed. The authors studied the properties of various internal combustion engines used for different applications (specialized engines of power generators—Liebherr G9512 and MAN E3262 LE212, powered by biogas, engine for road and off-road vehicles—Cummins 6C8.3, in self-ignition, original version powered by diesel fuel, and its modified version—a spark-ignition engine powered by methane fuel) under various operating conditions in approval tests. The sensitivity of the engine properties, especially pollutant emissions, to its operating states were studied. In the case of a Cummins 6C8.3 modified engine, a significant reduction in the pollutant emission owing to the use of methane fuel, relative to the original self-ignition engine, was found. The emission of carbon oxide decreased by approximately 30%, hydrocarbons by approximately 70% and nitrogen oxide by approximately 50%, as well as a particulate matter emission was also eliminated. Specific brake emission of carbon oxide is the most sensitive to the operating states of the engine: 0.324 for a self-ignition engine and 0.264 for a spark-ignition engine, with the least sensitive being specific brake emission of nitrogen oxide: 0.121 for a self-ignition engine and 0.097 for a spark-ignition engine. The specific brake emission of carbon monoxide and hydrocarbons for stationary engines was higher in comparison with both versions of Cummins 6C8.3 engine. However, the emission of nitrogen oxide for stationary engines was lower than for Cummins engines.
Integrated SMRT Technology with UMI RNA-Seq Reveals the Hub Genes in Stamen Petalody in Camellia oleifera
Authors: Li Huie , Hu Yang , Gao Chao , Guo Qiqiang , Deng Quanen , Nan Hong , Yang Lan , Wei Hongli , Qiu Jie , Yang Lu
Published: June 2021

Male sterility caused by stamen petalody is a key factor for a low fruit set rate and a low yield of Camellia oleifera but can serve as a useful genetic tool because it eliminates the need for artificial emasculation. However, its molecular regulation mechanism still remains unclear. In this study, transcriptome was sequenced and analyzed on two types of bud materials, stamen petalody mutants and normal materials, at six stages of stamen development based on integrated single-molecule real-time (SMRT) technology with unique molecular identifiers (UMI) and RNA-seq technology to identify the hub genes responsible for stamen petalody in C. oleifera. The results show that a large number of alternative splicing events were identified in the transcriptome. A co-expression network analysis of MADSs and all the differentially expressed genes between the mutant stamens and the normal materials showed that four MADS transcription factor genes, CoSEP3.1, CoAGL6, CoSEP3.2, and CoAP3, were predicted to be the hub genes responsible for stamen petalody. Among these four, the expression patterns of CoAGL6 and CoSEP3.2 were consistently high in the mutant samples, but relatively low in the normal samples at six stages, while the patterns of CoSEP3.1 and CoAP3 were initially low in mutants and then were upregulated during development but remained relatively high in the normal materials. Furthermore, the genes with high connectivity to the hub genes showed significantly different expression patterns between the mutant stamens and the normal materials at different stages. qRT-PCR results showed a similar expression pattern of the hub genes in the RNA-seq. These results lay a solid foundation for the directive breeding of C. oleifera varieties and provide references for the genetic breeding of ornamental Camellia varieties.
Genetic Diversity and Structure of Pinus densiflora Siebold & Zucc. Populations in Republic of Korea Based on Microsatellite Markers
Authors: Ahn Ji-Young , Lee Jei-Wan , Hong Kyung-Nak
Published: June 2021

Pinus densiflora Siebold & Zucc. is a widely distributed conifer species in the Republic of Korea with economic and ecologic importance. However, P. densiflora is negatively influenced by various factors, such as forest fires, clearing of large numbers of trees infected with Pinus disease, and dieback. We aimed to use microsatellite markers to estimate the genetic diversity, differentiation, and structure of P. densiflora populations in the Republic of Korea. A total of 1844 samples from 60 populations were evaluated using 11 polymorphic microsatellite markers. The observed heterozygosity and expected heterozygosity were 0.652 and 0.673, respectively. The mean genetic differentiation among the populations was 0.013. Moreover, P. densiflora showed high genetic diversity and low genetic differentiation compared with conifer species, including Pinus species with similar life histories. Principal coordinates analysis and Bayesian clustering showed that P. densiflora has a weak geographical structure. The P. densiflora population at Mt. Halla, Jeju Island, showed the lowest genetic diversity and significant genetic differentiation compared with other mainland populations due to genetic drift and restricted gene flow. These findings can be useful for designing new conservation, management, and breeding strategies for P. densiflora populations in response to future environmental changes.
Effects of the Addition of Herbs on the Properties of Doenjang
Authors: Lee Sunmin , Lee Yang-Bong , Lee Choong-Hwan , Park Inmyoung
Published: June 2021

Three types of doenjang, a fermented soybean paste, were prepared by adding coriander (CR), Korean mint (KM), and peppermint (PM) and compared to the control group (CN) by studying their metabolite profiles and antioxidant activities followed by different fermentation periods (1, 30, and 150 days, respectively). The primary metabolome was analyzed by GC-TOF-MS, and 36 of metabolites were identified in four types of doenjang samples (CN, CR, KM, and PM). Samples were clustered based on the herb type and fermentation period in PCA and PLS-DA analysis. For the secondary metabolome analysis, UHPLC-Q-orbitrap-MS was used, and 26 metabolites were identified. The statistical analysis showed that the samples were clustered by herb type rather than fermentation period, and the samples containing KM and PM were located in the same group. The DPPH assay showed that PM-containing doenjang had the highest antioxidant activity. Correlation analysis indicated that organic acids such as lactic acid, malonic acid, succinic acid, uracil, vanillic acid, and quinic acid showed positive correlation with the DPPH activity. Overall, our results demonstrated that incorporating herbs in doenjang during fermentation caused significant shifts (p-value < 0.05) in the doenjang metabolites and antioxidant activity. Hence, herbs could be utilized for enhancing doenjang fermentation.
Physicochemical Properties of Sago Ozone Oxidation: The Effect of Reaction Time, Acidity, and Concentration of Starch
Authors: Sumardiono Siswo , Jos Bakti , Pudjihastuti Isti , Yafiz Arvin M. , Rachmasari Megaria , Cahyono Heri
Published: June 2021

The disadvantageous properties of sago starch has limited its application in food and industrial processes. The properties of sago starch can be improved by changing its physicochemical and rheological characteristics. This study examined the influence of reaction time, acidity, and starch concentration on the oxidation of sago starch with ozone, a strong oxidant. Swelling, solubility, carbonyl, carboxyl, granule morphology, thermal profile, and functional groups are comprehensively observed parameters. With starch concentrations of 10–30% (v/w) and more prolonged oxidation, sago starch was most soluble at pH 10. The swelling power decreased with a longer reaction time, reaching the lowest pH 10. In contrast, the carbonyl and carboxyl content exhibited the same pattern as solubility. A more alkaline environment tended to create modified starch with more favorable properties. Over time, oxidation shows more significant characteristics, indicating a superb product of this reaction. At the starch concentration of 20%, modified sago starch with the most favorable properties was created. When compared to modified starch, native starch is generally shaped in a more oval and irregular manner. Additionally, native starch and modified starch had similar spectral patterns and identical X-ray diffraction patterns. Meanwhile, oxidized starch had different gelatinization and retrogradation temperatures to those of the native starch.
Defects in GnRH Neuron Migration/Development and Hypothalamic-Pituitary Signaling Impact Clinical Variability of Kallmann Syndrome
Authors: Kałużna Małgorzata , Budny Bartłomiej , Rabijewski Michał , Kałużny Jarosław , Dubiel Agnieszka , Trofimiuk-Müldner Małgorzata , Wrotkowska Elżbieta , Hubalewska-Dydejczyk Alicja , Ruchała Marek , Ziemnicka Katarzyna
Published: June 2021

Kallmann syndrome (KS) is a combination of isolated hypogonadotropic hypogonadism (IHH) with olfactory dysfunction, representing a heterogeneous disorder with a broad phenotypic spectrum. The genetic background of KS has not yet been fully established. This study was conducted on 46 Polish KS subjects (41 males, 5 females; average age: 29 years old). The studied KS patients were screened for defects in a 38-gene panel with next-generation sequencing (NGS) technology. The analysis revealed 27 pathogenic and likely pathogenic (P/LP) variants, and 21 variants of uncertain significance (VUS). The P/LP variants were detected in 20 patients (43.5%). The prevalence of oligogenic P/LP defects in selected genes among KS patients was 26% (12/46), whereas the co-occurrence of other variants was detected in 43% (20 probands). The examined KS patients showed substantial genotypic and phenotypic variability. A marked difference in non-reproductive phenotypes, involving defects in genes responsible for GnRH neuron development/migration and genes contributing to pituitary development and signaling, was observed. A comprehensive gene panel for IHH testing enabled the detection of clinically relevant variants in the majority of KS patients, which makes targeted NGS an effective molecular tool. The significance of oligogenicity and the high incidence of alterations in selected genes should be further elucidated.
High SGO2 Expression Predicts Poor Overall Survival: A Potential Therapeutic Target for Hepatocellular Carcinoma
Authors: Deng Min , Li Shaohua , Mei Jie , Lin Wenping , Zou Jingwen , Wei Wei , Guo Rongping
Published: June 2021

Shugoshin2 (SGO2) may participate in the occurrence and development of tumors by regulating abnormal cell cycle division, but its prognostic value in hepatocellular carcinoma (HCC) remains unclear. In this study, we accessed The Cancer Genome Atlas (TCGA) database to get the clinical data and gene expression profile of HCC. The expression of SGO2 in HCC tissues and nontumor tissues and the relationship between SGO2 expression, survival, and clinicopathological parameters were analyzed. The SGO2 expression level was significantly higher in HCC tissues than in nontumor tissues (p < 0.001). An analysis from the Oncomine and Gene Expression Profiling Interactive Analysis 2 (GEPIA2) databases also demonstrated that SGO2 was upregulated in HCC (all p < 0.001). A logistic regression analysis revealed that the high expression of SGO2 was significantly correlated with gender, tumor grade, pathological stage, T classification, and Eastern Cancer Oncology Group (ECOG) score (all p < 0.05). The overall survival (OS) of HCC patients with higher SGO2 expression was significantly poor (p < 0.001). A multivariate analysis showed that age and high expression of SGO2 were independent predictors of poor overall survival (all p < 0.05). Twelve signaling pathways were significantly enriched in samples with the high-SGO2 expression phenotype. Ten proteins and 34 genes were significantly correlated with SGO2. In conclusion, the expression of SGO2 is closely related to the survival of HCC. It may be used as a potential therapeutic target and prognostic marker of HCC.
A Homozygous Deletion of Exon 5 of KYNU Resulting from a Maternal Chromosome 2 Isodisomy (UPD2) Causes Catel-Manzke-Syndrome/VCRL Syndrome
Authors: Schüle Isabel , Berger Urs , Matysiak Uta , Ruzaike Gunda , Stiller Brigitte , Pohl Martin , Spiekerkoetter Ute , Lausch Ekkehart , Grünert Sarah C. , Schmidts Miriam
Published: June 2021

Vertebral, Cardiac, Renal and Limb Defect Syndrome (VCRL), is a very rare congenital malformation syndrome. Pathogenic variants in HAAO (3-Hydroxyanthranilate 3,4-dioxygenase), NADSYN1 (NAD+ Synthetase-1) and KYNU (Kynureninase) have been identified in a handful of affected individuals. All three genes encode for enzymes essential for the NAD+ de novo synthesis pathway. Using Trio-Exome analysis and CGH array analysis in combination with long range PCR, we have identified a novel homozygous copy number variant (CNV) encompassing exon 5 of KYNU in an individual presenting with overlapping features of VCRL and Catel–Manzke Syndrome. Interestingly, only the mother, not the father carried the small deletion in a heterozygous state. High-resolution SNP array analysis subsequently delineated a maternal isodisomy of chromosome 2 (UPD2). Increased xanthurenic acid excretion in the urine confirmed the genetic diagnosis. Our findings confirm the clinical, genetic and metabolic phenotype of VCRL1, adding a novel functionally tested disease allele. We also describe the first patient with NAD+ deficiency disorder resulting from a UPD. Furthermore, we provide a comprehensive review of the current literature covering the genetic basis and pathomechanisms for VCRL and Catel–Manzke Syndrome, including possible phenotype/genotype correlations as well as genetic causes of hypoplastic left heart syndrome.
Spatio-Seasonal Hypoxia/Anoxia Dynamics and Sill Circulation Patterns Linked to Natural Ventilation Drivers, in a Mediterranean Landlocked Embayment: Amvrakikos Gulf, Greece
Authors: Georgiou Nikos , Fakiris Elias , Koutsikopoulos Constantin , Papatheodorou George , Christodoulou Dimitris , Dimas Xenophon , Geraga Maria , Kapellonis Zacharias G. , Vaziourakis Konstantinos-Marios , Noti Alexandra , Antoniou Dimitra , Ferentinos George
Published: June 2021

Amvrakikos Gulf is a Mediterranean landlocked, fjord-like embayment and marine protected area suffering from natural, human-induced hypoxia/anoxia and massive fish mortality events. Seasonal marine geophysical and oceanographic surveys were conducted focusing on the water-circulation patterns at the sill and the spatial-seasonal distribution of dissolved oxygen (DO) in the gulf. Detailed surveys at the sill, the only communication route between the gulf and the open sea, revealed a two-layer water circulation pattern (top brackish outflow–bottom seawater inflow) and the role of the tide in the daily water exchange. Statistical analysis of the known natural drivers of DO distribution (density difference between the Ionian Sea and Amvrakikos, river inflow, wind) revealed that horizontal density gradients strongly affect anoxia reduction and seafloor oxygenation, while river inflow and wind mainly oxygenate volume/areas located above or within the pycnocline range, with DO concentrations > 2 mg/L. Complex geomorphology with well-formed internal basins contributes to the development and preservation of low DO conditions below the pycnocline. Finally, 43% of the seafloor and 36% of the gulf’s total water volume are permanently hypoxic, and reach a maximum of 70% and 62%, respectively, in September and July. This work is tailored to future ecosystem management plans, decisions, and future research on coastal ecosystems.
Female Patients with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome or Idiopathic Chronic Fatigue: Comparison of Responses to a Two-Day Cardiopulmonary Exercise Testing Protocol
Authors: van Campen C. (Linda) M. C. , Visser Frans C.
Published: June 2021

Introduction: Multiple studies have shown that peak oxygen consumption is reduced in the majority of ME/CFS patients, using the golden standard for measuring exercise intolerance: cardiopulmonary exercise testing (CPET). A 2-day CPET protocol has shown different results on day 2 in ME/CFS patients compared to sedentary controls. No comparison is known between ME/CFS and idiopathic chronic fatigue (ICF) for 2-day CPET protocols. We compared ME/CFS patients with patients with chronic fatigue who did not fulfil the ME/CFS criteria in a male population and hypothesized a different pattern of response would be present during the 2nd day CPET. Methods: Fifty-one female patients with ICF completed a 2-day CPET protocol and were compared to an age/sex-matched group of 50 female ME/CFS patients. Measures of oxygen consumption (VO2), heart rate (HR), systolic and diastolic blood pressure, workload (Work), and respiratory exchange ratio (RER) were collected at maximal (peak) and ventilatory threshold (VT) intensities. Results: Baseline characteristics for both groups were similar for age, BMI, BSA, and disease duration. A significance difference was present in the number of patients with fibromyalgia (seven ME/CFS patients vs zero ICF patients). Heart rate at rest and the RER did not differ significantly between CPET 1 and CPET 2. All other CPET parameters at the ventilatory threshold and maximum exercise differed significantly (p-value between 0.002 and <0.0001). ME/CFS patients showed a deterioration of performance on CPET2 as reflected by VO2 and workload at peak exercise and ventilatory threshold, whereas ICF patients showed improved performance on CPET2 with no significant change in peak workload. Conclusion: This study confirms that female ME/CFS patients have a reduction in exercise capacity in response to a second day CPET. These results are similar to published results in female ME/CFS populations. Patients diagnosed with ICF show a different response on day 2, more similar to sedentary and healthy controls.
The Critical Factors Affecting the Deployment and Scaling of Healthcare AI: Viewpoint from an Experienced Medical Center
Authors: Liu Chung-Feng , Huang Chien-Cheng , Wang Jhi-Joung , Kuo Kuang-Ming , Chen Chia-Jung
Published: June 2021

Healthcare Artificial Intelligence (AI) has the greatest opportunity for development. Since healthcare and technology are two of Taiwan’s most competitive industries, the development of healthcare AI is an excellent chance for Taiwan to improve its health-related services. From the perspective of economic development, promoting healthcare AI must be a top priority. However, despite having many breakthroughs in research and pilot projects, healthcare AI is still considered rare and is broadly used in the healthcare setting. Based on a medical center in Taiwan that has introduced a variety of healthcare AI into practice, this study discussed and analyzed the issues and concerns in the development and scaling of medical AIs from the perspective of various stakeholders in the healthcare setting, including the government, healthcare institutions, users (healthcare workers), and AI providers. The present study also identified critical influential factors for the deployment and scaling of healthcare AI. It is hoped that this paper can serve as an important reference for the advancement of healthcare AI not only in Taiwan but also in other countries.
Probiotics for the Prevention of Acute Respiratory-Tract Infections in Older People: Systematic Review
Authors: Strauss Maja , Mičetić-Turk Dušanka , Pogačar Maja Šikić , Fijan Sabina
Published: June 2021

The aim of this systematic review was to present the indirect influence of probiotics on the incidence and duration of acute upper respiratory-tract infections in older people, by regulating the immune system. Eight randomized, placebo-controlled clinical trials met the inclusion criteria, considering the threshold of older people being 60 years and over. Single strain probiotics were used in all studies, including three probiotic strains used in fermented foods: Lactobacillus delbrueckii subsp. bulgaricus OLL1073R-1, Lacticaseibacillus paracasei subsp. paracasei CNCM I-1518 and Lacticaseibacillusparacasei Shirota, and three probiotic strains used as food supplements: Loigolactobacillus coryniformis K8 CECT5711, Bacillus subtilis CU1 and Lacticaseibacillus rhamnosus GG. Current evidence showed that certain probiotic strains were better than a placebo in lowering the incidence or number of older people experiencing acute upper respiratory tract infections; however, not all probiotic strains were efficient, and not all studies reported statistically significant outcomes. More high quality large-scale properly controlled clinical studies focusing on older people are warranted.
Effects of Foliage Spraying with Sodium Bisulfite on the Photosynthesis of Orychophragmus violaceus
Authors: Li Zhongying , Wu Yanyou , Xing Deke , Zhang Kaiyan , Xie Jinjin , Yu Rui , Chen Tian , Duan Rongrong
Published: June 2021

Sulphurous acid derived from sulfur dioxide (SO2) emission leads to the pollution of irrigation water and the inhibition of plant growth. The safe concentration threshold of NaHSO3 in plants should be clarified to promote agricultural production. In this study, Orychophragmus violaceus seedlings were used as experimental materials and five NaHSO3 concentrations (i.e., 0, 1, 2, 5, 10 mmol·L−1) were simultaneously sprayed on the leaf surface of different seedlings separately. Leaf physiology responses under different concentrations were analyzed. The NaHSO3 did not promote photosynthesis in O. violaceus under the 1 and 2 mmol·L−1 treatments. It was conducive to the net photosynthetic rate (PN), photorespiration rate (Rp), chlorophyll content, actual photochemical quantum yield (YII) and photochemical quenching (qP) under the 5 mmol·L−1 treatment. However, quantum yield of regulated energy dissipation (YNPQ) and nonphotochemical quenching (NPQ) were inhibited. Under the 10 mmol·L−1 treatment, PN, chlorophyll content, YII, qP, dark respiration rate (Rd) and electron transport rate (ETR) showed significant decreases, while the photorespiration portion (Sp) significantly increased. Our results demonstrated that NaHSO3 provided a sulfur source for plant growth and interfered with the redox reaction of the plant itself, and its role as a photorespiratory inhibitor might be masked.
Effect of Daily Light Integral on Cucumber Plug Seedlings in Artificial Light Plant Factory
Authors: Cui Jiawei , Song Shiwei , Yu Jizhu , Liu Houcheng
Published: June 2021

In a controlled environment, in an artificial light plant factory during early spring or midsummer, vegetable seedlings can be uniform, compact, and high quality. Appropriate light parameters can speed up the growth of seedlings and save on production costs. Two experiments were carried out in this study: (1) cucumber seedling growth under different daily light integrals (DLIs) (5.41–11.26 mol·m−2·d−1) and optimum DLI for seedling production were explored (experiment 1: Exp. 1); (2) under the same DLI selected by Exp. 1, the effects of different light intensities and photoperiods on cucumber seedlings were investigated (experiment 2: Exp. 2). The root biomass, root-to-shoot ratio, seedling index, and shoot dry matter rate increased as the DLI increased from 5.41 to 11.26 mol·m−2·d−1, while the shoot biomass and leaf area decreased in Exp. 1. The cucumber seedlings became more compact as DLI increased, but more flowers developed after transplanting when the DLI was 6.35 mol·m−2·d−1. Under the optimal DLI (6.35 mol·m−2·d−1), the optimal intensity was 110–125 μmol·m−2·s−1, and the optimal photoperiod was 14–16 h, in which plant biomass, shoot dry matter rate, seedling index, and photochemical efficiency were higher.
The Impact of Salt Stress on Plant Growth, Mineral Composition, and Antioxidant Activity in Tetragonia decumbens Mill.: An Underutilized Edible Halophyte in South Africa
Authors: Sogoni Avela , Jimoh Muhali , Kambizi Learnmore , Laubscher Charles
Published: June 2021

Climate change, expanding soil salinization, and the developing shortages of freshwater have negatively affected crop production around the world. Seawater and salinized lands represent potentially cultivable areas for edible salt-tolerant plants. In the present study, the effect of salinity stress on plant growth, mineral composition (macro-and micro-nutrients), and antioxidant activity in dune spinach (Tetragonia decumbens) were evaluated. The treatments consisted of three salt concentrations, 50, 100, and 200 mM, produced by adding NaCl to the nutrient solution. The control treatment had no NaCl but was sustained and irrigated by the nutrient solution. Results revealed a significant increase in total yield, branch production, and ferric reducing antioxidant power in plants irrigated with nutrient solution incorporated with 50 mM NaCl. Conversely, an increased level of salinity (200 mM) caused a decrease in chlorophyll content (SPAD), while the phenolic content, as well as nitrogen, phosphorus, and sodium, increased. The results of this study indicate that there is potential for brackish water cultivation of dune spinach for consumption, especially in provinces experiencing the adverse effect of drought and salinity, where seawater or underground saline water could be diluted and used as irrigation water in the production of this vegetable.
COVID-19 in Dental Settings: Novel Risk Assessment Approach
Authors: Alsaegh Ali , Belova Elena , Vasil’ev Yuriy , Zabroda Nadezhda , Severova Lyudmila , Timofeeva Margarita , Dobrokhotov Denis , Leonova Alevtina , Mitrokhin Oleg
Published: June 2021

The novel coronavirus (COVID-19) outbreak is a public health emergency of international concern, and this emergency led to postponing elective dental care procedures. The postponing aimed to protect the public from an unknown risk caused by COVID-19. At the beginning of the outbreak, for public health authorities, the aerosol-generating procedures and the close proximity between dental care workers and patients in dentistry represented sufficient justification for the delay of dental visits. Dental care is a priority, and for many years, studies have proven that the lack and delay of dental care can cause severe consequences for the oral health of the general population, which can cause a high global burden of oral diseases. Safety is necessary while resuming dental activities, and risk assessment is an efficient method for understanding and preventing the COVID-19 infectious threats facing the dental industry and affecting dental care workers and patients. In this study, for safe dental care delivery, we adapted risk assessment criteria and an approach and an occupational classification system. Based on those tools, we also recommend measures that can help to minimize infectious risk in dental settings.
Factors That Cause Concerns after Cardioverter Defibrillator Implantation
Authors: Karczewska Olimpia , Młynarska Agnieszka
Published: June 2021

Background and Objectives: The aim of the study was to assess the factors that influence the occurrence of concerns and their intensification after the implantation of a cardioverter defibrillator. Materials and Methods: This was a prospective and observational study including 158 patients. The study was conducted in two stages: stage I before implantable cardioverter defibrillator (ICD) implantation and stage II follow-up visit six months after ICD implantation. Standardized questionnaires were used in both stages. Results: Age and female gender were significantly correlated with the occurrence and intensity of concerns. Patients who had a device implanted for secondary prevention also experienced higher levels of concern. Additionally, a multiple regression model using the stepwise input method was performed. The model was statistically significant and explained 42% of the observed variance in the dependent variable (p = 0.0001, R2 = 0.4215). The analysis showed that age (p = 0.0036), insomnia (p = 0.0276), anxiety (p = 0.0000) and negative emotions (p = 0.0374) were important predictors of the dependent variable and enabled higher levels of the number of concerns to be predicted. Conclusions: There is a relationship between the severity of the concerns related to an implanted ICD and age, gender, anxiety, negative emotions and insomnia. Indications for ICD implantation may be associated with increased concerns about ICD.