bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of demineralization and ball milling treatments on the properties of Arundo donax and olive stone-derived biochar
Authors: Zouari M. , Marrot L. , DeVallance D. B.
Published: May 2023

The structural and physio-chemical properties of biochar are crucial to determining biochar’s quality and the adequate application. Specifically, the large porosity of biochar has been known as a favorable feature, especially for environmental remediation. In this regard, physical and chemical modifications have been used to improve biochar’s porosity which requires high-energy consumption and involves chemical agents. The objective of this study was to prepare biochar with developed porosity using mild treatments. Arundo donax and olive stone were demineralized by a water-washing method. Treated and non-treated biomasses were pyrolyzed, and part of the derived samples was subjected to wet ball milling. Samples were characterized with proximate, Fourier transform infrared, particle size, and physisorption analyses. The effect of demineralization depended on the biomass type, as ash reduction only influenced Arundo donax-derived biochar, which was attributed to the difference in initial ash content that was relatively low for olive stone. The carbonization yield decreased by 46% for the Arundo donax biomass after demineralization. Moreover, demineralization expanded the surface area and total pore volume of the Arundo donax biochar. The ball milling was effective in producing micro-sized biochar particles with a mean size ranging between 30 ± 2 µm and 42 ± 2 µm and between 13 ± 1 µm and 22 ± 2 µm for Arundo donax and olive stone without and with demineralization, respectively. Ball milling increased the surface area of non-demineralized Arundo donax by 47% and demineralized Arundo donax by 124%. Additionally, ball milling increased the surface area of non-demineralized olive stone by 65% and demineralized olive stone by 62%.

Graphical abstract
Dynamics of excitable cells: spike-adding phenomena in action
Authors: Barrio Roberto , Ibáñez Santiago , Jover-Galtier Jorge A. , Lozano Álvaro , Martínez M. Ángeles , Mayora-Cebollero Ana , Mayora-Cebollero Carmen , Pérez Lucía , Serrano Sergio , Vigara Rubén
Published: May 2023

We study the dynamics of action potentials of some electrically excitable cells: neurons and cardiac muscle cells. Bursting, following a fast–slow dynamics, is the most characteristic behavior of these dynamical systems, and the number of spikes may change due to spike-adding phenomenon. Using analytical and numerical methods we give, by focusing on the paradigmatic 3D Hindmarsh–Rose neuron model, a review of recent results on the global organization of the parameter space of neuron models with bursting regions occurring between saddle-node and homoclinic bifurcations (fold/hom bursting). We provide a generic overview of the different bursting regimes that appear in the parametric phase space of the model and the bifurcations among them. These techniques are applied in two realistic frameworks: insect movement gait changes and the appearance of Early Afterdepolarizations in cardiac dynamics.
How to Talk Language Science with Everybody
Authors: Wagner Laura , McKee Cecile
Published: May 2023

Do you want to talk about the linguistic research that you think is important but you don't know where to start? Language is a topic that is relevant to everyone, and linguists are often asked to speak publicly about their research, to a range of lay audiences in the media, politics, festivals and fairs, schools, museums and public libraries. However, relaying this vital information in an engaging way can often feel like an insurmountable task. This accessible guide offers practical advice on how to talk about language to a range of non-academic audiences. It draws on the linguistics behind effective communication to help you have cooperative conversations, and to organize your information for a diverse range of people. It is illustrated with a wealth of examples from real-life scenarios, and includes chapter-by-chapter worksheets, enabling you to make your own fun and interesting language science activities to share with others.
Scaling laws for planetary sediment transport from DEM-RANS numerical simulations
Authors: Pähtz Thomas , Durán Orencio
Published: May 2023

We use an established discrete element method (DEM) Reynolds-averaged Navier–Stokes (RANS)-based numerical model to simulate non-suspended sediment transport across conditions encompassing almost seven orders of magnitude in the particle–fluid density ratio$s$, ranging from subaqueous transport ($s=2.65$) to aeolian transport in the highly rarefied atmosphere of Pluto ($s=10^7$), whereas previous DEM-based sediment transport studies did not exceed terrestrial aeolian conditions ($s\approx 2000$). Guided by these simulations and by experiments, we semi-empirically derive simple scaling laws for the cessation threshold and rate of equilibrium aeolian transport, both exhibiting a rather unusual$s^{1/3}$-dependence. They constitute a simple means to make predictions of aeolian processes across a large range of planetary conditions. The derivation consists of a first-principle-based proof of the statement that, under relatively mild assumptions, the cessation threshold physics is controlled by only one dimensionless control parameter, rather than two expected from dimensional analysis. Crucially, unlike existing models, this proof does not resort to coarse-graining the particle phase of the aeolian transport layer above the bed surface. From the pool of existing models, only that by Pähtzet al.(J. Geophys. Res.: Earth, vol. 126, 2021, e2020JF005859) is somewhat consistent with the combined numerical and experimental data. It captures the scaling of the cessation threshold and the$s^{1/3}$-dependence of the transport rate, but fails to capture the latter's superimposed grain size dependence. This hints at a lack of understanding of the transport rate physics and calls for future studies on this issue.
The generalized Clapeyron equation and its application to confined ice growth
Authors: Style Robert W. , Gerber Dominic , Rempel Alan W. , Dufresne Eric R.
Published: May 2023

Most theoretical descriptions of stresses induced by freezing are rooted in the (generalized) Clapeyron equation, which predicts the pressure that a solid can exert as it cools below its melting temperature. This equation is central for topics ranging beyond glaciology to geomorphology, civil engineering, food storage and cryopreservation. However, it has inherent limitations, requiring isotropic solid stresses and conditions near bulk equilibrium. Here, we examine when the Clapeyron equation is applicable by providing a rigorous derivation that details all assumptions. We demonstrate the natural extension for anisotropic stress states, and we show how the temperature and pressure ranges for validity depend on well-defined material properties. Finally, we demonstrate how the range of applicability of the (linear) Clapeyron equation can be extended by adding higher-order terms, yielding results that are in good agreement with experimental data for the pressure melting of ice.
Beyond the conservative hypothesis: a meta-analysis of lexical-semantic processing in Williams syndrome
Authors: Romero-Rivas Carlos , Rodríguez-Cuadrado Sara , Sabater Lucía , Rodríguez Gómez Pablo , Hidalgo de la Guía Irene , Moreno Eva M. , Garayzábal Heinze Elena
Published: May 2023

Williams syndrome (WS) is a rare genetic disorder, characterised at the cognitive level by a phenotypic pattern of relative weaknesses (e.g., visuospatial skills) and strengths (e.g., some linguistic and nonverbal reasoning skills). In this study, we performed a systematic search and meta-analysis on lexical-semantic processing in WS, an area of knowledge in which contradictory results have been obtained. We found 42 studies matching our criteria, and, in total, 78 effect sizes were included in the meta-analysis. Results showed that individuals with WS have worse lexical-semantic skills than individuals with typical development, whether matched by chronological or mental age. However, people with WS have better lexical-semantic skills than people diagnosed with other cognitive disabilities. Finally, vocabulary skills seem to be relatively spared in WS, although they present some difficulties in semantic processing/integration, semantic memory organisation and verbal working memory skills. Taken together, these results support a neuroconstructivist approach, according to which the cognitive mechanisms involved in lexical-semantic processing may be modulated, even when performance in some tasks (i.e., vocabulary tasks) might be optimal.
Interrelation of radiocarbon ages from bone fractions in the Brazilian Intertropical Region
Authors: Dantas Mário André Trindade , Cherkinsky Alexander
Published: May 2023

There is a consensus in the literature that radiocarbon dating performed on bioapatite often produces ages younger than dating performed on collagen. We propose a general regression that could be used to convert the bioapatite radiocarbon ages to the simulated ages on collagen in fossil samples worldwide. This general regression presents several good indices of quality, high correlation (R2 = 0.98), lower values of percent predicted error (%PE = 0.01), and standard error of the estimate (%SEE = 21.83), showing that it is a good tool, as the predicted values are similar to those observed. Using this regression, we converted the radiocarbon ages of bioapatite to the expected age from the collagen fraction made for several taxa from the Brazilian Intertropical Region (BIR) and suggest that these dates could be 1–7 cal ka BP older than previously thought.
A systematic review of the role of penicillin versus penicillin plus metronidazole in the management of peritonsillar abscess
Authors: Moen C M , Paramjothy K , Williamson A , Coleman H , Lou X , Smith A , Douglas C M
Published: May 2023

Background

Peritonsillar abscess is a localised infection in the peritonsillar space. Pus from the abscess can contain anaerobes. Many clinicians prescribe metronidazole in addition to penicillin, but evidence to support this is limited. This review assessed the evidence of benefit of metronidazole for the treatment of peritonsillar abscess.

Methods

A systematic review was conducted of the literature and databases including Ovid Medline, Ovid Embase, PubMed and Cochrane library. Search terms included all variations of peritonsillar abscess, penicillin and metronidazole.

Results

Three randomised, control trials were included. All studies assessed the clinical outcomes after treatment for peritonsillar abscess, including recurrence rate, length of hospital stay and symptom improvement. There was no evidence to suggest additional benefit with metronidazole, with studies suggesting increased side effects.

Conclusion

Evidence does not support the addition of metronidazole in first-line management of peritonsillar abscess. Further trials to establish optimum dose and duration schedules of oral phenoxymethylpenicillin would benefit clinical practice.
Osedax (Siboglinidae: Annelida) utilizes shark teeth for nutrition
Authors: Rouse Greg W. , Goffredi Shana K.
Published: May 2023

We deployed jaws of the common thresher shark (Alopias vulpinus) on the seafloor at ~1000 m depth off Monterey California for 8 months. The jaws disintegrated, with all the hyaline cartilage disappearing, leaving some fragments of tessellated cartilage and the teeth. Two different Osedax species, O. packardorum and O. talkovici were found to have bored into the roots of some of the teeth, and were using the dentin pulp, which is rich in collagen, as a food source. The enameloid crowns of the shark teeth and the tessellated cartilage showed no signs of Osedax activity. This is the first demonstration of Osedax exploiting a source of food that is not bone. This raises questions as to the original food source of Osedax ‘bone worms'. Examination for the presence of Osedax in the skeletons and teeth of Mesozoic and possibly even Palaeozoic fossil sharks, bony fish and reptiles is warranted.
Diet and feeding of juvenile common two-banded sea bream, Diplodus vulgaris (Teleostei: Sparidae), in the eastern central Adriatic Sea
Authors: Krstulović Šifner Svjetlana , Šantić Mate
Published: May 2023

The diet and feeding of juvenile common two-banded sea bream, Diplodus vulgaris, in the eastern central Adriatic Sea was studied to better understand local ecosystem dynamics in this region. Stomach contents of 140 individuals with total length (TL) between 22 and 106 mm, collected by small beach seines from February to November, were analysed. Food items identified in stomachs belonged to 16 prey groups: Copepoda, Gastropoda, Teleost eggs, Ostracoda, Polychaeta, Bivalvia, unidentified Crustacea, Amphipoda, Decapoda, Cumacea, Echinoidea, Anisopoda, Euphausiacea, Mysidacea, Branchiopoda and Isopoda. Overall, planktonic copepod crustaceans were the most important prey group (percentage index of relative importance, %IRI = 78.9), followed by gastropods (%IRI = 14.9). All other prey groups had much lower %IRI values and thus were of less importance. Fish size was an important factor influencing food composition. Planktonic copepods were the most important prey in juveniles of smaller sizes (up to 76 mm TL), whereas large-sized juvenile individuals (>76 mm TL) mainly consumed benthic prey, such as gastropods, polychaetes and bivalves. Feeding intensity was very high as indicated by the low vacuity index.
Blunted neurobiological reactivity and attentional bias to threat underlie stress-related disorders in women survivors of intimate partner violence
Authors: Goldberg X. , Espelt C. , Nadal R. , Alon Y. , Palao D. , Bar-Haim Y. , Armario A.
Published: May 2023

Background

Intimate partner violence (IPV) alters women's neurobiological stress response systems. We propose that individual differences early in the attentional processing of threats are associated with these neurobiological mechanisms and contribute to mental illness in this population.

Methods

We assessed attentional bias in relation to threat (AB) in women survivors of IPV (n = 69) and controls (n = 36), and examined overall cortisol secretion using hair cortisol (HC), and stress responsiveness measuring salivary cortisol and α-amylase (sAA) before (T0), and after (T1, T2) an acute psychosocial stress task (Trier Social Stress Test). We used repeated-measures ANCOVAs to explore the associations between Group (IPV, control) and AB with acute stress response, and regression models to examine the associations with mental health symptoms.

Results

There were no between-group differences in HC levels. An interaction between Group and AB was found regarding cortisol reactivity (p < 0.05). IPV women with threat avoidance AB showed a blunted cortisol response compared to controls and to IPV participants with threat vigilance AB. The association between sAA reactivity and the interaction between Group, AB, and time approached significance (p = 0.07), with a trend to lower sAA levels particularly in IPV women with threat avoidance AB. Group and cortisol reactivity were associated with symptoms of depression, generalized anxiety, and post-traumatic stress disorder (8–20% explained variance).

Conclusions

Threat avoidance AB is associated with blunted acute cortisol response among women exposed to chronic stress (IPV). Experiencing IPV and acute cortisol response appear to be clearly implicated in long-term mental health problems.
Catastrophic health expenditure and the risk of depression among middle-aged and old people in China: a national population-based longitudinal study
Authors: Wang Yaping , Liu Min , Liu Jue
Published: May 2023

Aims

To estimate the association of catastrophic health expenditure (CHE) with the risk of depression in middle-aged and old people in China.

Methods

We used data of 2011, 2013, 2015 and 2018 from the China Health and Retirement Longitudinal Study, which covered 150 counties of 28 provinces in China. CHE was calculated as out-of-pocket health expenditure exceeding 40% of a household’s capacity to pay. Depression was measured by a 10-item Centre for Epidemiological Studies Depression Scale. We evaluated CHE prevalence and applied Cox proportional hazard models to estimate adjusted hazard ratios (aHRs) and 95% confident intervals (CIs) for the risk of depression among participants with CHE after controlling potential confounders, compared with those without CHE.

Results

Among 5765 households included in this study, CHE prevalence at baseline was 19.24%. The depression incidence of participants with CHE (8.00 per 1000 person-month) was higher than that of those without CHE (6.81 per 1000 person-month). After controlling confounders, participants with CHE had a 13% higher risk (aHR = 1.13, 95% CI: 1.02–1.26) of depression than those without CHE. In subgroup analysis, the association of CHE with depression was significant in males and in people with chronic diseases, of younger age, living in rural areas and of lowest family economic level (all P < 0.05).

Conclusions

Nearly one of five middle-aged and old people in China incurred CHE, and CHE was associated with the risk of depression. Concerted efforts should be made to monitor CHE and related depression episode. Moreover, timely interventions about CHE and depression need to be implemented and strengthened among middle-aged and old people.
A Rapid Systematic Review of the Prevalence of Suicide and Self-Harm Behaviors in Adolescents During the COVID-19 Pandemic
Authors: Sahoo Swapnajeet , Patra Suravi
Published: May 2023

Abstract: Background: COVID-19 has caused psychological, social, and physical isolation in adolescents resulting in varying rates of suicidal behavior and self-harm. Aims: We investigated the pandemic's impact on adolescent suicidal behavior and self-harm by reviewing the existing literature. Methods: We searched PubMed using keywords: adolescent, suicide, suicidal behavior, self-harm, prevalence, and COVID-19 and included studies reporting primary data only. Results: Of the 551 studies identified, we included 39 studies in the final analysis. Two of the six high-quality population-based suicide registry studies reported increased suicide rates during the pandemic. Seven of fifteen emergency department–based studies out of which four were of high quality and three high-quality population-based health registry studies reported increased self-harm. A few school and community-based surveys and national helpline data also reported an increase in suicidal behavior or self-harm. Limitations: Methodological heterogeneity of the included studies. Conclusions: There is wide variation in study methodology, population, settings, and age groups in the included studies. Suicidal behavior and self-harm were increased in specific study settings and adolescent populations during the pandemic. More methodologically rigorous research is needed to evaluate the impact of COVID-19 on adolescent suicidal behavior and self-harm.
Understanding Nighttime Ionospheric Depletions Associated With Sudden Stratospheric Warmings in the American Sector
Authors: Jones M. , Goncharenko L. P. , McDonald S. E. , Zawdie K. A. , Tate J. , Gasperini F. , Pedatella N. M. , Drob D. P. , McCormack J. P.
Published: May 2023

This study focuses on understanding what drives the previously observed deep nighttime ionospheric hole in the American sector during the January 2013 sudden stratospheric warming (SSW). Performing a set of numerical experiments with the thermosphere‐ionosphere‐mesosphere‐electrodynamics general circulation model (TIME‐GCM) constrained by a high‐altitude version of the Navy Global Environmental Model, we demonstrate that this nighttime ionospheric hole was the result of increased poleward and down magnetic field line plasma motion at low and midlatitudes in response to altered F‐region neutral meridional winds. Thermospheric meridional wind modifications that produced this nighttime depletion resulted from the well‐known enhancements in semidiurnal tidal amplitudes associated with stratospheric warming (SSWs) in the upper mesosphere and thermosphere. Investigations into other deep nighttime ionospheric depletions and their cause were also considered. Measurements of total electron content from Global Navigation Satellite System receivers and additional constrained TIME‐GCM simulations showed that nighttime ionospheric depletions were also observed on several nights during the January‐February 2010 SSW, which resulted from the same forcing mechanisms as those observed in January 2013. Lastly, the recent January 2021 SSW was examined using Modern‐Era Retrospective Analysis for Research and Applications, Version 2, COSMIC‐2 Global Ionospheric Specification electron density, and ICON Michelson Interferometer for Global High‐Resolution Thermospheric Imaging horizontal wind data and revealed a deep nighttime ionospheric depletion in the American sector was likely driven by modified meridional winds in the thermosphere. The results shown herein highlight the importance of thermospheric winds in driving nighttime ionospheric variability over a wide latitude range.
Positive Feedback Between the Tidal Flat Variations and Sediment Dynamics: An Example Study in the Macro‐Tidal Turbid Hangzhou Bay
Authors: Li Li , Ren Yihan , Ye Taoyan , Wang Xiao Hua , Hu Jianyu , Xia Yuezhang
Published: May 2023

Tidal flat variations (curvature) in macro‐tidal turbid bays highly correlate with sediment dynamics. Interactions between tidal flats and sediment dynamics in macro‐tidal turbid estuaries, like Hangzhou Bay, China, have been studied through a fully validated 3D sediment model by considering high‐turbidity, flocculation, and water‐sediment density coupling. Numerical results show that the circulation and sediment flux in the curvature of Andong tidal flat have similar magnitudes in the along‐estuarine and lateral directions, demonstrating the important impact of the geometry on hydrodynamics and sediment transport processes. Sediment moves toward the southern/northern bank near the bottom/surface level in curvature. Tidally averaged net sediment flux is southward in the curvature. Tidal flat reclamation changes the curvature of the tidal channel, and modulates the circulation and sediment transport, and then feeds back to the tidal flat evolution subsequently. Bottom friction, nonlinear advection, and centrifugal force are dominant forces for inducing the lateral circulation, followed by the Coriolis force and sediment‐induced baroclinic process. Lateral circulation combined with the suspended sediment concentration (SSC) profile controls the distribution of instantaneous and tidally averaged net sediment fluxes in the curvature. The sediment flux positively correlates with the curvature of channel bends. The increased curvature of channel bends enhances the southward sediment fluxes mainly by increasing the effects of the centrifugal force, nonlinear advection, and Coriolis force on the lateral circulation and SSC. This study revealed a two‐way feedback system between tidal flat and sediment dynamics, which contributes to the impact of anthropogenic activities on geomorphological evolution of similar estuaries worldwide.
Quantitative Mineralogy of Planetary Silicate Ternary Mixtures Using Raman Spectroscopy
Authors: Qi Xiaobin , Ling Zongcheng , Liu Ping , Chen Jian , Cao Haijun , Liu Changqing , Wang Xiaoyu , Liu Yiheng
Published: May 2023

A 532‐nm‐excited lunar Raman spectrometer (LRS) has been selected as a scientific payload of the Chang'e‐7 mission, exploring mineralogy assemblages in the lunar south polar region. However, the quantification of dark‐colored silicate minerals via Raman spectroscopy is an urgent requirement for upcoming Raman applications in future lunar and planetary explorations. Therefore, we conducted detailed laboratory studies on the Raman quantification of lunar silicate minerals using ternary mixtures of feldspar, olivine, and augite. Quantitative models were established employing the observed linear relationship between Raman integrated intensities and mineral proportions. The significant correlation coefficients (>0.94) and small RMSE (≤4.20 wt.%) confirmed the performance of these models. A series of methods (multipoint sampling, multispectral averaging, peak area extraction, and spectral parameter ratios) were jointly used to ensure that the models were not significantly affected by crystal orientation, chemical inhomogeneity, and instruments. Factors (σ2/σ1) describing the relative Raman scattering cross sections were introduced to calibrate the Raman counts. Our results indicated that the relative Raman scattering efficiency of feldspar, olivine, and augite is 1.4:2.4:1, which can be used to improve the quantitative accuracy of the point‐counting method if polymineralic mixing spectra are dominant. The models were validated across different samples using laboratory mixtures and lunar soil (CE5C0600). The lithology of the Chang'e‐5 soils is basaltic/gabbroic according to the quantitative mineralogy returned from our models that is consistent with the results from traditional methods. This research will be of particular significance for accurately determining the mineral abundances for Chang'e‐7 and other planetary missions. As such, crucial information can be inferred to understand the geological evolution of the exploration regions.
Satellite‐Based Long‐Term Spatiotemporal Trends in Ambient NO2 Concentrations and Attributable Health Burdens in China From 2005 to 2020
Authors: Huang Keyong , Zhu Qingyang , Lu Xiangfeng , Gu Dongfeng , Liu Yang
Published: May 2023

Despite the recent development of using satellite remote sensing to predict surface NO2 levels in China, methods for estimating reliable historical NO2 exposure, especially before the establishment of NO2 monitoring network in 2013, are still rare. A gap‐filling model was first adopted to impute the missing NO2 column densities from satellite, then an ensemble machine learning model incorporating three base learners was developed to estimate the spatiotemporal pattern of monthly mean NO2 concentrations at 0.05° spatial resolution from 2005 to 2020 in China. Further, we applied the exposure data set with epidemiologically derived exposure response relations to estimate the annual NO2 associated mortality burdens in China. The coverage of satellite NO2 column densities increased from 46.9% to 100% after gap‐filling. The ensemble model predictions had good agreement with observations, and the sample‐based, temporal and spatial cross‐validation (CV) R2 were 0.88, 0.82, and 0.73, respectively. In addition, our model can provide accurate historical NO2 concentrations, with both by‐year CV R2 and external separate year validation R2 achieving 0.80. The estimated national NO2 levels showed a increasing trend during 2005–2011, then decreased gradually until 2020, especially in 2012–2015. The estimated annual mortality burden attributable to long‐term NO2 exposure ranged from 305 thousand to 416 thousand, and varied considerably across provinces in China. This satellite‐based ensemble model could provide reliable long‐term NO2 predictions at a high spatial resolution with complete coverage for environmental and epidemiological studies in China. Our results also highlighted the heavy disease burden by NO2 and call for more targeted policies to reduce the emission of nitrogen oxides in China.
CD301b+ macrophage: the new booster for activating bone regeneration in periodontitis treatment
Authors: Wang Can , Zhao Qin , Chen Chen , Li Jiaojiao , Zhang Jing , Qu Shuyuan , Tang Hua , Zeng Hao , Zhang Yufeng
Published: May 2023

Periodontal bone regeneration is a major challenge in the treatment of periodontitis. Currently the main obstacle is the difficulty of restoring the regenerative vitality of periodontal osteoblast lineages suppressed by inflammation, via conventional treatment. CD301b+ macrophages were recently identified as a subpopulation that is characteristic of a regenerative environment, but their role in periodontal bone repair has not been reported. The current study indicates that CD301b+ macrophages may be a constituent component of periodontal bone repair, and that they are devoted to bone formation in the resolving phase of periodontitis. Transcriptome sequencing suggested that CD301b+ macrophages could positively regulate osteogenesis-related processes. In vitro, CD301b+ macrophages could be induced by interleukin 4 (IL-4) unless proinflammatory cytokines such as interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) were present. Mechanistically, CD301b+ macrophages promoted osteoblast differentiation via insulin-like growth factor 1 (IGF-1)/thymoma viral proto-oncogene 1 (Akt)/mammalian target of rapamycin (mTOR) signaling. An osteogenic inducible nano-capsule (OINC) consisting of a gold nanocage loaded with IL-4 as the “core” and mouse neutrophil membrane as the “shell” was designed. When injected into periodontal tissue, OINCs first absorbed proinflammatory cytokines in inflamed periodontal tissue, then released IL-4 controlled by far-red irradiation. These events collectively promoted CD301b+ macrophage enrichment, which further boosted periodontal bone regeneration. The current study highlights the osteoinductive role of CD301b+ macrophages, and suggests a CD301b+ macrophage-targeted induction strategy based on biomimetic nano-capsules for improved therapeutic efficacy, which may also provide a potential therapeutic target and strategy for other inflammatory bone diseases.
New genetic and epigenetic insights into the chemokine system: the latest discoveries aiding progression toward precision medicine
Authors: Xu Hanli , Lin Shuye , Zhou Ziyun , Li Duoduo , Zhang Xiting , Yu Muhan , Zhao Ruoyi , Wang Yiheng , Qian Junru , Li Xinyi , Li Bohan , Wei Chuhan , Chen Keqiang , Yoshimura Teizo , Wang Ji Ming , Huang Jiaqiang
Published: May 2023

Over the past thirty years, the importance of chemokines and their seven-transmembrane G protein-coupled receptors (GPCRs) has been increasingly recognized. Chemokine interactions with receptors trigger signaling pathway activity to form a network fundamental to diverse immune processes, including host homeostasis and responses to disease. Genetic and nongenetic regulation of both the expression and structure of chemokines and receptors conveys chemokine functional heterogeneity. Imbalances and defects in the system contribute to the pathogenesis of a variety of diseases, including cancer, immune and inflammatory diseases, and metabolic and neurological disorders, which render the system a focus of studies aiming to discover therapies and important biomarkers. The integrated view of chemokine biology underpinning divergence and plasticity has provided insights into immune dysfunction in disease states, including, among others, coronavirus disease 2019 (COVID-19). In this review, by reporting the latest advances in chemokine biology and results from analyses of a plethora of sequencing-based datasets, we outline recent advances in the understanding of the genetic variations and nongenetic heterogeneity of chemokines and receptors and provide an updated view of their contribution to the pathophysiological network, focusing on chemokine-mediated inflammation and cancer. Clarification of the molecular basis of dynamic chemokine-receptor interactions will help advance the understanding of chemokine biology to achieve precision medicine application in the clinic.
Cell activation-based screening of natively paired human T cell receptor repertoires
Authors: Fahad Ahmed S. , Chung Cheng Yu , López Acevedo Sheila N. , Boyle Nicoleen , Madan Bharat , Gutiérrez-González Matías F. , Matus-Nicodemos Rodrigo , Laflin Amy D. , Ladi Rukmini R. , Zhou John , Wolfe Jacy , Llewellyn-Lacey Sian , Koup Richard A. , Douek Daniel C. , Balfour Henry H. , Price David A. , DeKosky Brandon J.
Published: May 2023

Adoptive immune therapies based on the transfer of antigen-specific T cells have been used successfully to treat various cancers and viral infections, but improved techniques are needed to identify optimally protective human T cell receptors (TCRs). Here we present a high-throughput approach to the identification of natively paired human TCRα and TCRβ (TCRα:β) genes encoding heterodimeric TCRs that recognize specific peptide antigens bound to major histocompatibility complex molecules (pMHCs). We first captured and cloned TCRα:β genes from individual cells, ensuring fidelity using a suppression PCR. We then screened TCRα:β libraries expressed in an immortalized cell line using peptide-pulsed antigen-presenting cells and sequenced activated clones to identify the cognate TCRs. Our results validated an experimental pipeline that allows large-scale repertoire datasets to be annotated with functional specificity information, facilitating the discovery of therapeutically relevant TCRs.
Effects of exogenous organic matter addition on agricultural soil microbial communities and relevant enzyme activities in southern China
Authors: Liu Xing , Chen Qi , Zhang Huicheng , Zhang Jiaen , Chen Yuting , Yao Fucheng , Chen Yingtong
Published: May 2023

Soil microbial community composition plays a key role in the decomposition of organic matter, while the quality of exogenous organic matter (EOM: rice straw, roots and pig manure) can influence soil chemical and biological properties. However, the evidences of the effect of combination of crop residues and pig manure on the changes in soil microbial community and enzymes activities are scarce. A greenhouse pot experiment was conducted to investigate the potential effect of EOM by analyzing soil properties, enzyme activities and microbial communities. The experiment consisted of eight treatments: CK (control), S (1% (w/w) rice straw), R (1% (w/w) rice root), SR (1% (w/w) rice straw + 1% (w/w) rice root), and added 1% (w/w) pig manure to CK, S, R and SR, respectively. Results showed that the straw treatment significantly increased the microbial biomass (carbon and nitrogen) and total carbon and nitrogen contents, cellulase and β-1,4-glucosidase activities, bacteria (i.e., gram-positive bacteria and gram-negative bacteria) PLFAs contents relative to CK regardless of whether pig manure was added. Moreover, the interaction between crop residues (e.g., straw and roots) and pig manure significantly influenced the contents of microbial biomass nitrogen and microbial biomass phosphorus, and the ratio of gram-positive bacteria to gram-negative bacteria. Redundance analysis confirmed that pH, nitrate nitrogen, ammonium nitrogen and dissolve organic carbon contents were significantly associated with soil microbial community under crop residues without pig manure addition. Furthermore, the experiment results showed that pig manure application not only provided more abundant nutrients (C, N and P) but also induced higher microbial and enzymatic activity compared with no pig manure addition. Our findings suggest that the combination of above-ground straw and pig manure is a better option for improving the functions of soil ecosystem.
Stability of silicon–tin alloyed nanocrystals with high tin concentration synthesized by femtosecond laser plasma in liquid media
Authors: Lozac’h Mickaël , Bürkle Marius , McDonald Calum , Miyadera Tetsuhiko , Koganezawa Tomoyuki , Mariotti Davide , Švrček Vladimir
Published: May 2023

Nanocrystals have a great potential for future materials with tunable bandgap, due to their optical properties that are related with the material used, their sizes and their surface termination. Here, we concentrate on the silicon–tin alloy for photovoltaic applications due to their bandgap, lower than bulk Si, and also the possibility to activate direct band to band transition for high tin concentration. We synthesized silicon–tin alloy nanocrystals (SiSn-NCs) with diameter of about 2–3 nm by confined plasma technique employing a femtosecond laser irradiation on amorphous silicon–tin substrate submerged in liquid media. The tin concentration is estimated to be $$\sim 17\%$$

∼
17
%

, being the highest Sn concentration for SiSn-NCs reported so far. Our SiSn-NCs have a well-defined zinc-blend structure and, contrary to pure tin NCs, also an excellent thermal stability comparable to highly stable silicon NCs. We demonstrate by means of high resolution synchrotron XRD analysis (SPring 8) that the SiSn-NCs remain stable from room temperature up to $$400\,^{\circ }\text {C},$$

400

∘

C
,

with a relatively small expansion of the crystal lattice. The high thermal stability observed experimentally is rationalized by means of first-principle calculations.
Diagnostic role of aortic valve calcium scoring in various etiologies of aortic stenosis
Authors: Wanchaitanawong Wisarut , Kanjanavanit Rungsrit , Srisuwan Tanop , Wongcharoen Wanwarang , Phrommintikul Arintaya
Published: May 2023

Most of the studies about aortic valve calcium (AVC) score in aortic stenosis (AS) were based on degenerative or bicuspid AS but not rheumatic AS. We aimed to study the diagnostic accuracy of AVC score to determine severe AS in various etiologies. Adult patients diagnosed with mild to severe AS were enrolled. AVC score were identified from multi-detector computed tomography (MDCT) scan. The AVC score was highest in bicuspid AS (3211.9 (IQR (1100.0–4562.4) AU) compared to degenerative AS (1803.7 (IQR (1073.6–2550.6) AU)), and rheumatic AS (875.6 (IQR 453.3–1594.0) AU), p < 0.001. For the ROC curve to identify severe AS, the AVC score performed well in degenerative and bicuspid AS with the area under the ROC curve (AuROC) 0.834 (95% CI, 0.730, 0.938) in degenerative group; and 0.820 (95% CI, 0.687, 0.953) in bicuspid AS. Whereas AVC score had non-significant diagnostic accuracy with AuROC 0.667 (95% CI, 0.357, 0.976) for male and 0.60(95% CI, 0.243, 0.957) for female in rheumatic AS. The cut-off AVC score values to identify severe AS were AVCS > 2028.9AU (male) and > 1082.5AU (female) for degenerative AS, and > 2431.8AU (male) and > 1293.5AU (female) for bicuspid AS. In conclusions, AVC score is the accurate test for assessing severity in patients with degenerative and bicuspid AS but performs poorly in rheumatic AS group.
Structural and functional analyses of the echinomycin resistance conferring protein Ecm16 from Streptomyces lasalocidi
Authors: Gade Priyanka , Erlandson Amanda , Ullah Anwar , Chen Xi , Mathews Irimpan I. , Mera Paola E. , Kim Chu-Young
Published: May 2023

Echinomycin is a natural product DNA bisintercalator antibiotic. The echinomycin biosynthetic gene cluster in Streptomyces lasalocidi includes a gene encoding the self-resistance protein Ecm16. Here, we present the 2.0 Å resolution crystal structure of Ecm16 bound to adenosine diphosphate. The structure of Ecm16 closely resembles that of UvrA, the DNA damage sensor component of the prokaryotic nucleotide excision repair system, but Ecm16 lacks the UvrB-binding domain and its associated zinc-binding module found in UvrA. Mutagenesis study revealed that the insertion domain of Ecm16 is required for DNA binding. Furthermore, the specific amino acid sequence of the insertion domain allows Ecm16 to distinguish echinomycin-bound DNA from normal DNA and link substrate binding to ATP hydrolysis activity. Expression of ecm16 in the heterologous host Brevibacillus choshinensis conferred resistance against echinomycin and other quinomycin antibiotics, including thiocoraline, quinaldopeptin, and sandramycin. Our study provides new insight into how the producers of DNA bisintercalator antibiotics fend off the toxic compounds that they produce.
A muti-informant national survey on the impact of COVID-19 on mental health symptoms of parent–child dyads in Canada
Authors: Leigh Jeanna Parsons , Moss Stephana Julia , Sriskandarajah Cynthia , McArthur Eric , Ahmed Sofia B. , Birnie Kathryn , Halperin Donna , Halperin Scott , Harley Micaela , Hu Jia , Ng Kamstra Josh , Leppan Laura , Nickel Angie , Racine Nicole , Russell Kristine , Smith Stacie , Solis May , Stelfox Maia , Tutelman Perri R. , Stelfox Henry T. , Fiest Kirsten M.
Published: May 2023

The COVID-19 pandemic negatively impacted the mental health of children, youth, and their families which must be addressed and prevented in future public health crises. Our objective was to measure how self-reported mental health symptoms of children/youth and their parents evolved during COVID-19 and to identify associated factors for children/youth and their parents including sources accessed for information on mental health. We conducted a nationally representative, multi-informant cross-sectional survey administered online to collect data from April to May 2022 across 10 Canadian provinces among dyads of children (11–14 years) or youth (15–18 years) and a parent (> 18 years). Self-report questions on mental health were based on The Partnership for Maternal, Newborn & Child Health and the World Health Organization of the United Nations H6+ Technical Working Group on Adolescent Health and Well-Being consensus framework and the Coronavirus Health and Impact Survey. McNemar’s test and the test of homogeneity of stratum effects were used to assess differences between children-parent and youth-parent dyads, and interaction by stratification factors, respectively. Among 933 dyads (N = 1866), 349 (37.4%) parents were aged 35–44 years and 485 (52.0%) parents were women; 227 (47.0%) children and 204 (45.3%) youth were girls; 174 (18.6%) dyads had resided in Canada < 10 years. Anxiety and irritability were reported most frequently among child (44, 9.1%; 37, 7.7%) and parent (82, 17.0%; 67, 13.9%) dyads, as well as among youth (44, 9.8%; 35, 7.8%) and parent (68, 15.1%; 49, 10.9%) dyads; children and youth were significantly less likely to report worsened anxiety (p < 0.001, p = 0.006, respectively) or inattention (p < 0.001, p = 0.028, respectively) compared to parents. Dyads who reported financial or housing instability or identified as living with a disability more frequently reported worsened mental health. Children (96, 57.1%), youth (113, 62.5%), and their parents (253, 62.5%; 239, 62.6%, respectively) most frequently accessed the internet for mental health information. This cross-national survey contextualizes pandemic-related changes to self-reported mental health symptoms of children, youth, and families.
Apigenin attenuates atherosclerosis and non-alcoholic fatty liver disease through inhibition of NLRP3 inflammasome in mice
Authors: Lu Zheng , Liu Lu , Zhao Shunxin , Zhao Jiangtao , Li Sujun , Li Mingyang
Published: May 2023

Apigenin (APN), a flavone found in several plant foods with various biological properties such as anti-obesity, anti-inflammation and other abilities, alleviates atherosclerosis and non-alcoholic fatty liver disease (NAFLD) induced by a high fat diet (HFD) in mice. However, the underlying mechanisms have not been fully understood. In this study, we investigated the role of NLRP3 in anti-atherosclerosis and anti-NAFLD effect of APN in mouse models with NLRP3 deficiency. Atherosclerosis and NAFLD models were established by treatment of low density lipoprotein receptor-deficient (Ldlr−/−) mice and NLRP3−/− Ldlr−/− mice with a HFD diet (20% fat and 0.5% cholesterol) with or without APN. En face lipid accumulation analysis, plasma lipid levels, hepatic lipid accumulation and inflammation were analyzed and quantified. For in vitro experiments, HepG2 cells were stimulated by LPS plus oleic acid (OA) in the absence or presence of APN (50 μM). Lipid accumulation and the effect of APN on the NLRP3/NF-κB signaling pathway were investigated. APN administration partly reversed atherosclerosis and hepatic lipid accumulation, and decreased body weight and plasma lipid levels in Ldlr−/− mice when fed a HFD. Compared with Ldlr−/− mice, NLRP3−/− Ldlr−/− mice showed more severe atherosclerosis and hepatic lipid accumulation. Treating the HepG2 cells with APN reduced lipid accumulation. APN also inhibited activation of the NLRP3/ NF-κB signaling pathway stimulated by OA together with LPS. Our results indicate that APN supplementation prevents atherosclerosis and NAFLD via NLRP3 inhibition in mice, and suggest that APN might be a potential therapeutic agent for the prevention of atherosclerosis and NAFLD.
Comparative appraisal of mono and hybrid nanofluid flows comprising carbon nanotubes over a three-dimensional surface impacted by Cattaneo–Christov heat flux
Authors: Alharbi Khalid Abdulkhaliq M. , Ramzan Muhammad , Shahmir Nazia , Ghazwani Hassan Ali S. , Elmasry Yasser , Eldin Sayed M. , Bilal Muhammad
Published: May 2023

Carbon nanotubes (CNTs) are nanoscale tubes made of carbon atoms with unique mechanical, electrical, and thermal properties. They have a variety of promising applications in electronics, energy storage, and composite materials and are found as single-wall carbon nanotubes (SWCNTs) and double-wall carbon nanotubes (DWCNTs). Considering such alluring attributes of nanotubes, the motive of the presented flow model is to compare the thermal performance of magnetohydrodynamic (MHD) mono (SWCNTs)/Ethylene glycol) and hybrid (DWCNTs- SWCNTs/Ethylene glycol) nanofluids over a bidirectional stretching surface. The thermal efficiency of the proposed model is gauged while considering the effects of Cattaneo-Christov heat flux with prescribed heat flux (PHF) and prescribed surface temperature (PST). The flow is assisted by the anisotropic slip at the boundary of the surface. The system of partial differential equations (PDEs) is converted into a nonlinear ordinary differential system by the use of similarity transformations and handled using the bvp4c numerical technique. To depict the relationship between the profiles and the parameters, graphs, and tables are illustrated. The significant outcome revealed that the fluid temperature rises in the scenario of both PST and PHF cases. In addition, the heat transfer efficiency of the hybrid nanoliquid is far ahead of the nanofluid flow. The truthfulness of the envisioned model in the limiting scenario is also given.
Metal chalcogenides (CuS or MoS2)-modified TiO2 as highly efficient bifunctional photocatalyst nanocomposites for green H2 generation and dye degradation
Authors: El-Gendy Reem A. , El-Bery Haitham M. , Farrag Mostafa , Fouad Dina M.
Published: May 2023

Herein, we report the modification of TiO2nanostructures with two different metal chalcogenides (CuS or MoS2). The effect of the preparation scheme (hydrothermal and coprecipitation methods) and the mass ratio of metal chalcogenides were investigated. The as-synthesized photocatalyst nanocomposites were fully characterized by various techniques. Moreover, the photo/electrochemical analysis were performed to investigate the photoelectric properties and photocatalytic mechanism. The photocatalytic performance was evaluated using two test reactions. In the case of H2generation via water splitting, it was found that 0.5 wt% CuS-TiO2synthesized via the coprecipitation method exhibited an initial hydrogen evolution rate (HER) of 2.95 mmol h−1 g−1. While, the optimized 3 wt% MoS2-TiO2synthesized by the hydrothermal method, showed an HER of 1.7 mmol h−1 g−1. Moreover, the degradation efficiency of methylene blue dye was 98% under UV–Vis light irradiation within 2 h over 0.5 CT_PP and 3MT_HT. Under visible irradiation, the degradation efficiency was 100% and 96% for 3MT_PP and 0.5CT_HT in the presence of H2O2, respectively. This study has proven that metal chalcogenides can act as effective, stable, and low-cost bifunctional co-catalysts to enhance the overall photocatalytic performance.