bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical Significance of Measuring Global Hydroxymethylation of White Blood Cell DNA in Prostate Cancer: Comparison to PSA in a Pilot Exploratory Study
Authors: Grelus Alin , Nica Dragos , Miklos Imola , Belengeanu Valerica , Ioiart Ioan , Popescu Cristina
Published: November 2017

This is the first study investigating the clinical relevance of 5-hydroxymethylcytosine (5hmC) in genomic DNA from white blood cells (WBC) in the context of prostate cancer (PCa) and other prostate pathologies. Using an enzyme-linked immunosorbent assay, we identified significantly different distributions of patients with low and elevated 5hmC content in WBC DNA across controls and patients with prostate cancer (PCa), atypical small acinar proliferation (ASAP), and benign prostatic hyperplasia (BPH). The measured values were within the normal range for most PCa patients, while the latter category was predominant for ASAP. We observed a wider heterogeneity in 5hmC content in all of the prostate pathologies analyzed when compared to the healthy age-matched controls. When compared to blood levels of prostate-specific antigen (PSA), this 5hmC-based biomarker had a lower performance in PCa detection than the use of a PSA cut-off of 2.5 nanograms per milliliter (ng/mL). Above this threshold, however, it delineated almost three quarters of PCa patients from controls and patients with other prostate pathologies. Overall, genome-wide 5hmC content of WBC DNA appears to be applicable for detecting non-cancerous prostate diseases, rather than PCa. Our results also suggest a potential clinical usefulness of complementing PSA as a PCa marker by the addition of a set of hydroxymethylation markers in the blood, but further studies are necessary to confirm these findings.
Short-Term Choriocapillaris Changes in Patients with Central Serous Chorioretinopathy after Half-Dose Photodynamic Therapy
Authors: Nassisi Marco , Lavia Carlo , Alovisi Camilla , Musso Luca , Eandi Chiara
Published: November 2017

Background: Although photodynamic therapy (PDT) has become the standard treatment for central serous chorioretinopathy (CSC), its mechanism of action remains unclear. It is assumed that PDT induces short-term choriocapillaris (CC) occlusion and long-term choroidal vascular remodeling. In this paper, we describe the short-term CC changes induced by Half-Dose PDT (HD-PDT) in chronic CSC using optical coherence tomography-angiography (OCTA). Methods: This is a prospective interventional case series. Chronic CSC eyes underwent Spectral-Domain OCT, Fundus Autofluorescence, FA, ICGA (Heidelberg Spectralis, Heidelberg, Germany) and OCTA (RTVue XR Avanti with AngioVue; Optovue Inc., Fremont, CA, USA) before HD-PDT, with follow-up after one hour, one week, and one month. Vascular changes after PDT were analyzed within the CC layer. The CC vessel density was defined as the percentage of an area occupied by flow pixels, using Image J software to obtain measurements by applying a grey level threshold. All pixels with a grey level above the threshold were considered as indicators of blood flow. Results: 20 eyes of 19 patients were included. At baseline the mean CC vessel density was 94.87 ± 2.32%. It significantly differed from the density at 1 week and 1 month (92.79 ± 3.16% and 95.55 ± 2.05%, p < 0.001, respectively), but not with values at 1 h (94.8 ± 2.28%, p = 0.516). Conclusions: CC vessel density was significantly reduced at 1 week as compared with baseline, suggesting a possible short-term effect of PDT on CC perfusion. After 1 month however, the CC vessel density was even higher than the baseline, probably due to a CC recovery. OCTA seems to be useful in the visualization of CC vessels and in confirming the mechanism of action of PDT treatment in eyes with chronic CSC.
Is the Benefit–Risk Ratio for Patients with Transfusion-Dependent Thalassemia Treated by Unrelated Cord Blood Transplantation Favorable?
Authors: Jaing Tang-Her
Published: November 2017

Transfusion-dependent thalassemia (TDT) is an inherited disorder characterized by absent or defective production of α- or β-hemoglobin chains. If untreated, the disease invariably culminates in death in early infancy due to cardiac failure or overwhelming infection. Although there is clear evidence of good health-related quality of life and return to normal life style, the choice to undergo hematopoietic stem cell transplantation (HSCT) remains a challenge because of the potential risk of transplant-related mortality (TRM) in TDT. Successful hematopoietic stem cell transplantation may cure the hematological manifestations of TDT, but introduces risks of TRM and morbidity. The low incidence of graft-versus-host disease (GVHD) provides the major rationale for pursuing unrelated cord blood transplantation (CBT). Considerable evidence suggests a lower rate of recurrence after CBT than after transplantation from adult donors. As the TRM, overall survival, and thalassemia-free survival for CBT improve, the utility of this stem cell source will expand to indications that have hitherto rarely used unrelated CBT. This paper summarizes the current progress in understanding the advances in unrelated CBT for thalassemia. Although as yet only in a limited number of patients, the results of unrelated CBT for thalassemia are encouraging.
Akt1 Stimulates Homologous Recombination Repair of DNA Double-Strand Breaks in a Rad51-Dependent Manner
Authors: Mueck Katharina , Rebholz Simone , Harati Mozhgan Dehghan , Rodemann H. Peter , Toulany Mahmoud
Published: November 2017

Akt1 is known to promote non-homologous end-joining (NHEJ)-mediated DNA double-strand break (DSB) repair by stimulation of DNA-PKcs. In the present study, we investigated the effect of Akt1 on homologous recombination (HR)-dependent repair of radiation-induced DSBs in non-small cell lung cancer (NSCLC) cells A549 and H460. Akt1-knockdown (Akt1-KD) significantly reduced Rad51 protein level, Rad51 foci formation and its colocalization with γH2AX foci after irradiation. Moreover, Akt1-KD decreased clonogenicity after treatment with Mitomycin C and HR repair, as tested by an HR-reporter assay. Double knockdown of Akt1 and Rad51 did not lead to a further decrease in HR compared to the single knockdown of Rad51. Consequently, Akt1-KD significantly increased the number of residual DSBs after irradiation partially independent of the kinase activity of DNA-PKcs. Likewise, the number of residual BRCA1 foci, indicating unsuccessful HR events, also significantly increased in the irradiated cells after Akt1-KD. Together, the results of the study indicate that Akt1 seems to be a regulatory component in the HR repair of DSBs in a Rad51-dependent manner. Thus, based on this novel role of Akt1 in HR and the previously described role of Akt1 in NHEJ, we propose that targeting Akt1 could be an effective approach to selectively improve the killing of tumor cells by DSB-inducing cytotoxic agents, such as ionizing radiation.
Takamatsu, H. Comparison of Minimal Residual Disease Detection by Multiparameter Flow Cytometry, ASO-qPCR, Droplet Digital PCR, and Deep Sequencing in Patients with Multiple Myeloma Who Underwent Autologous Stem Cell Transplantation. J. Clin. Med. 2017, 6, 91
Authors: Takamatsu Hiroyuki
Published: November 2017

The author wishes to make the following correction to this paper [...]
Identifying Hot Spots of Critical Forage Supply in Dryland Nomadic Pastoralist Areas: A Case Study for the Afar Region, Ethiopia
Authors: Sonneveld B. G. J. S. , Van Wesenbeeck C. F. A. , Keyzer M. A. , Beyene F. , Georgis K. , Urbano F. , Meroni M. , Leo O. , Yimer M. K. , Abdullatif M.
Published: November 2017

This study develops a methodology to identify hot spots of critical forage supply in nomadic pastoralist areas, using the Afar Region, Ethiopia, as a special case. It addresses two main problems. First, it makes a spatially explicit assessment of fodder supply and demand extracted from a data poor environment. Fodder supply is assessed by combining rainfall-based production functions and rule-based assessment for prevailing land use. Fodder demand is based on a data consistency check of livestock statistics concerning herd size, composition and geographical distribution. Second, individual herd movements have to be evaluated jointly in concurrent migration patterns to assess local pressures on fodder resources. We, therefore, apply a transition model that relates stock levels to seasonal migration routings for all Afar sub-clans jointly so as to localize the hot spots where feed demand exceeds forage supply. Critical areas come to the fore, especially, near fringes of Highlands and in the southern part of the Afar. A sensitivity test shows that ‘Baseline’ scenario is close to the ‘Best’ but under ‘Worst’, the Afar region would fall into despair. We conclude that the model is a useful tool to inform policy makers on critical areas in the Afar region.
Influence of Shenxiong Glucose Injection on the Activities of Six CYP Isozymes and Metabolism of Warfarin in Rats Assessed Using Probe Cocktail and Pharmacokinetic Approaches
Authors: Sun Jia , Lu Yuan , Li Yueting , Pan Jie , Liu Chunhua , Gong Zipeng , Huang Jing , Zheng Jiang , Zheng Lin , Li Yongjun , Liu Ting , Wang Yonglin
Published: November 2017

Shenxiong glucose injection (SGI), a traditional Chinese medicine (TCM) preparation, has been widely used for the treatment of various cardiovascular and cerebrovascular diseases for many years. We assessed the potential influences of SGI on the activities of six CYP enzymes (CYP1A2, CYP2C11, CYP2C19, CYP2D4, CYP2E1, and CYP3A2) and on the pharmacokinetics of warfarin in rats. We compared plasma pharmacokinetics of six probe drugs (caffeine/CYP1A2, tolbutamide/CYP2C11, omeprazole/CYP2C19, metoprolol/CYP2D4, chlorzoxazone/CYP2E1, and midazolam/CYP3A2) and of warfarin between control and SGI-pretreated groups, to estimate the effect on the relative activities of the six isozymes and warfarin metabolism. There were no significant differences in the pharmacokinetic parameters of caffeine, omeprazole, metoprolol, chlorzoxazone, and midazolam between the SGI-pretreated and control groups. However, many pharmacokinetic parameters of tolbutamide in SGI-pretreated rats were affected significantly (p < 0.05), and indicated tolbutamide metabolism in the former group was markedly slower. Moreover, SGI reduced the clearance of warfarin. These results suggested SGI showed no effects on the enzyme activities of rat CYP1A2, CYP2C19, CYP2D4, CYP2E1, and CYP3A2, but inhibited the enzyme activity of CYP2C11, and improved the blood concentration of warfarin. This suggests that the dose of warfarin may need be adjusted when co-administrated with SGI.
Identification of Major Flavone C-Glycosides and Their Optimized Extraction from Cymbidium kanran Using Deep Eutectic Solvents
Authors: Jeong Kyung , Yang Misuk , Jin Yan , Kim Eun , Ko Jaeyoung , Lee Jeongmi
Published: November 2017

Cymbidium kanran, an orchid exclusively distributed in Northeast Asia, has been highly valued as a decorative plant and traditional herbal medicine. Here, C. kanran extracts were prepared in 70% aqueous methanol using ultrasound-assisted extraction (UAE) and subjected to liquid chromatography-photodiode array detection and ultra-high performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry analysis, which were used for quantitative and qualitative analysis, respectively. It was found that the extracts were rich in flavone C-glycosides including vicenin-2, vicenin-3, schaftoside, vitexin, and isovitexin. Ten deep eutectic solvents (DESs) were synthesized by combining choline chloride (hydrogen bond acceptor) with various polyols and diols (hydrogen bond donors) and were tested as a medium for the efficient production of extracts enriched with potentially bioactive flavone C-glycosides from C. kanran. A DES named ChCl:DPG, composed of choline chloride and dipropylene glycol at a 1:4 molar ratio, exhibited the best extraction yields. Then, the effects of extraction conditions on the extraction efficiency were investigated by response surface methodology. Lower water content in the extraction solvent and longer extraction time during UAE were desirable for higher extraction yields. Under the statistically optimized conditions, in which 100 mg of C. kanran powder were extracted in 0.53 mL of a mixture of ChCl:DPG and water (74:26, w/w) for 86 min, a total of 3.441 mg g−1 flavone C-glycosides including 1.933 mg g−1 vicenin-2 was obtained. This total yield was 196%, 131%, and 71% more than those obtained using 100% methanol, water, and 70% methanol, respectively.
Studies for Improving a Rat Model of Alzheimer’s Disease: Icv Administration of Well-Characterized β-Amyloid 1-42 Oligomers Induce Dysfunction in Spatial Memory
Authors: Kasza Ágnes , Penke Botond , Frank Zsuzsanna , Bozsó Zsolt , Szegedi Viktor , Hunya Ákos , Németh Klaudia , Kozma Gábor , Fülöp Lívia
Published: November 2017

During the past 15 years, several genetically altered mouse models of human Alzheimer’s disease (AD) have been developed. These costly models have greatly facilitated the evaluation of novel therapeutic approaches. Injecting synthetic β-amyloid (Aβ) 1-42 species into different parts of the brain of non-transgenic rodents frequently provided unreliable results, owing to a lack of a genuine characterization of the administered Aβ aggregates. Previously, we have published a new rat AD-model in which protofibrillar-fibrillar Aβ1-42 was administered into rat entorhinal cortex (Sipos 2007). In order to develop a more reliable model, we have injected well-characterized toxic soluble Aβ1-42 species (oligomers, protofibrils and fibrils) intracerebroventricularly (icv) into rat brain. Studies of the distribution of fluorescent-labeled Aβ1-42 in the brain showed that soluble Aβ-species diffused into all parts of the rat brain. After seven days, the Aβ-treated animals showed a significant decrease of spatial memory in Morris water maze test and impairment of synaptic plasticity (LTP) measured in acute hippocampal slices. The results of histological studies (decreased number of viable neurons, increased tau levels and decreased number of dendritic spines) also supported that icv administration of well-characterized toxic soluble Aβ species into rat brain provides a reliable rat AD-model.
Synthesis and Improved Cross-Linking Properties of C5-Modified Furan Bearing PNAs
Authors: Elskens Joke , Manicardi Alex , Costi Valentina , Madder Annemieke , Corradini Roberto
Published: November 2017

Over the past decades, peptide nucleic acid/DNA (PNA:DNA) duplex stability has been improved via backbone modification, often achieved via introducing an amino acid side chain at the α- or γ-position in the PNA sequence. It was previously shown that interstrand cross-linking can further enhance the binding event. In this work, we combined both strategies to fine-tune PNA crosslinking towards single stranded DNA sequences using a furan oxidation-based crosslinking method; for this purpose, γ-l-lysine and γ-l-arginine furan-PNA monomers were synthesized and incorporated in PNA sequences via solid phase synthesis. It was shown that the l-lysine γ-modification had a beneficial effect on crosslink efficiency due to pre-organization of the PNA helix and a favorable electrostatic interaction between the positively-charged lysine and the negatively-charged DNA backbone. Moreover, the crosslink yield could be optimized by carefully choosing the type of furan PNA monomer. This work is the first to describe a selective and biocompatible furan crosslinking strategy for crosslinking of γ-modified PNA sequences towards single-stranded DNA.
Comparison of the Chemical Profiles and Antioxidant Activities of Different Parts of Cultivated Cistanche deserticola Using Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry and a 1,1-Diphenyl-2-picrylhydrazyl-Based Assay
Authors: Wang Xiaoming , Wang Jinfang , Guan Huanyu , Xu Rong , Luo Xiaomei , Su Meifeng , Chang Xiaoyan , Tan Wenting , Chen Jun , Shi Yue
Published: November 2017

In this study, a sensitive ultra-performance liquid chromatography-photodiode array coupled to quadruple time-of-flight mass (UPLC-PDA-Q/TOF-MS) method and a 1,1-diphenyl-2- picrylhydrazyl (DPPH)-based assay were used to determine the chemical constituents and screen the antioxidant activity profiles of the methanol extracts of different parts of cultivated Cistanche deserticola (C. deserticola). First, qualitative and quantitative chemical composition analyses of the different parts of cultivated C. deserticola were conducted. Obvious differences were observed between the chemical profiles and content distribution of phenylethanoid glycosides (PhGs) from the different cultivated C. deserticola parts. The average contents of the six PhGs parts varied from 4.91 to 72.56 mg/g DW (milligrams of extract per gram of plant dry weight) in the six different parts of Cistanche deserticola, displaying a significant decreasing trend from the bottom to the top of cultivated C. deserticola and the highest content in the stems. From the bottom to the top of the plant, the echinacoside and cistanoside A content decreased and the 2 ′ -acetylacteoside content increased. Second, an offline DPPH assay revealed that the total scavenging activities of all parts within the range of 20–500 μ g/mL increased in a concentration-dependent manner and that good antioxidant activities were found in all plant parts, particularly in the stems, which could be related to their higher PhG content. Additionally, a DPPH-UPLC-PDA method was successfully applied to rapidly screen the antioxidant profiles and antioxidant components of the different cultivated C. deserticola parts. According to the antioxidant profiles before and after the DPPH reaction, there were wide variations in the antioxidant activities of different cultivated C. deserticola parts. Moreover, the antioxidant profiles revealed the presence of major free radical scavengers identified as PhGs using UPLC-Q/TOF-MS. Finally, the established DPPH-UPLC-PDA method was reagent saving, rapid and feasible for correlating the chemical profile of traditional chinese medicines (TCMs) with their bioactivities without isolation and purification and may be used for multicomponent analysis of active substances in other foods and herbs. Therefore, to better harness C. deserticola resources, using this method to evaluate cultivated C. deserticola, a promising herb material with obvious antioxidant activity, is crucial.
Assembly of the Cutin Polyester: From Cells to Extracellular Cell Walls
Authors: Bakan Bénédicte , Marion Didier
Published: November 2017

Cuticular matrices covering aerial plant organs or delimiting compartments in these organs are composed of an insoluble hydrophobic polymer of high molecular mass, i.e., cutin, that encompass some cell wall polysaccharides and is filled by waxes. Cutin is a polyester of hydroxy and-or epoxy fatty acids including a low amount of glycerol. Screening of Arabidopsis and more recently of tomato (Solanum lycopersicum) mutants allowed the delineation of the metabolic pathway involved in the formation of cutin monomers, as well as their translocation in the apoplast. Furthermore, these studies identified an extracellular enzyme involved in the polymerization of these monomers, i.e., cutin synthase 1 (CUS1), an acyl transferase of the GDSL lipase protein family. By comparing the structure of tomato fruit cutins from wild type and down-regulated CUS1 mutants, as well as with the CUS1-catalyzed formation of oligomers in vitro, hypothetical models can be elaborated on the polymerization of cutins. The polymorphism of the GDSL-lipase family raises a number of questions concerning the function of the different isoforms in relation with the formation of a composite material, the cuticle, containing entangled hydrophilic and hydrophobic polymers, i.e., polysaccharides and cutin, and plasticizers, i.e., waxes.
Assembled Cantilever Fiber Touch Trigger Probe for Three-Dimensional Measurement of Microstructures
Authors: Zou Limin , Ni He , Zhang Peng , Ding Xuemei
Published: November 2017

In this paper, an assembled cantilever fiber touch trigger probe was developed for three-dimensional measurements of clear microstructures. The probe consists of a shaft assembled vertically to an optical fiber cantilever and a probing sphere located at the free end of the shaft. The laser is emitted from the free end of the fiber cantilever and converges on the photosensitive surface of the camera through the lens. The position shift of the light spot centroid was used to detect the performance of the optical fiber cantilever, which changed dramatically when the probing sphere touched the objects being measured. Experimental results indicated that the sensing system has sensitivities of 3.32 pixels/μm, 1.35 pixels/μm, and 7.38 pixels/μm in the x, y, and z directions, respectively, and resolutions of 10 nm, 30 nm, and 5 nm were achieved in the x, y, and z, respectively. An experiment on micro slit measurement was performed to verify the high aspect ratio measurement capability of the assembled cantilever fiber (ACF) probe and to calibrate the effective two-point diameter of the probing sphere. The two-point probe sphere diameter was found to be 174.634 μm with a standard uncertainly of 0.045 μm.
Wireless Monitoring of Induction Machine Rotor Physical Variables
Authors: Doolan Fernandes Jefferson , Carvalho Souza Francisco , Cipriano Maniçoba Glauco , Salazar Andrés , De Paiva José
Published: November 2017

With the widespread use of electric machines, there is a growing need to extract information from the machines to improve their control systems and maintenance management. The present work shows the development of an embedded system to perform the monitoring of the rotor physical variables of a squirrel cage induction motor. The system is comprised of: a circuit to acquire desirable rotor variable(s) and value(s) that send it to the computer; a rectifier and power storage circuit that converts an alternating current in a continuous current but also stores energy for a certain amount of time to wait for the motor’s shutdown; and a magnetic generator that harvests energy from the rotating field to power the circuits mentioned above. The embedded system is set on the rotor of a 5 HP squirrel cage induction motor, making it difficult to power the system because it is rotating. This problem can be solved with the construction of a magnetic generator device to avoid the need of using batteries or collector rings and will send data to the computer using a wireless NRF24L01 module. For the proposed system, initial validation tests were made using a temperature sensor (DS18b20), as this variable is known as the most important when identifying the need for maintenance and control systems. Few tests have shown promising results that, with further improvements, can prove the feasibility of using sensors in the rotor.
System Modeling of a MEMS Vibratory Gyroscope and Integration to Circuit Simulation
Authors: Kwon Hyukjin , Seok Seyeong , Lim Geunbae
Published: November 2017

Recently, consumer applications have dramatically created the demand for low-cost and compact gyroscopes. Therefore, on the basis of microelectromechanical systems (MEMS) technology, many gyroscopes have been developed and successfully commercialized. A MEMS gyroscope consists of a MEMS device and an electrical circuit for self-oscillation and angular-rate detection. Since the MEMS device and circuit are interactively related, the entire system should be analyzed together to design or test the gyroscope. In this study, a MEMS vibratory gyroscope is analyzed based on the system dynamic modeling; thus, it can be mathematically expressed and integrated into a circuit simulator. A behavioral simulation of the entire system was conducted to prove the self-oscillation and angular-rate detection and to determine the circuit parameters to be optimized. From the simulation, the operating characteristic according to the vacuum pressure and scale factor was obtained, which indicated similar trends compared with those of the experimental results. The simulation method presented in this paper can be generalized to a wide range of MEMS devices.
Survey on the Performance of Source Localization Algorithms
Authors: Fresno José , Robles Guillermo , Martínez-Tarifa Juan , Stewart Brian
Published: November 2017

The localization of emitters using an array of sensors or antennas is a prevalent issue approached in several applications. There exist different techniques for source localization, which can be classified into multilateration, received signal strength (RSS) and proximity methods. The performance of multilateration techniques relies on measured time variables: the time of flight (ToF) of the emission from the emitter to the sensor, the time differences of arrival (TDoA) of the emission between sensors and the pseudo-time of flight (pToF) of the emission to the sensors. The multilateration algorithms presented and compared in this paper can be classified as iterative and non-iterative methods. Both standard least squares (SLS) and hyperbolic least squares (HLS) are iterative and based on the Newton–Raphson technique to solve the non-linear equation system. The metaheuristic technique particle swarm optimization (PSO) used for source localisation is also studied. This optimization technique estimates the source position as the optimum of an objective function based on HLS and is also iterative in nature. Three non-iterative algorithms, namely the hyperbolic positioning algorithms (HPA), the maximum likelihood estimator (MLE) and Bancroft algorithm, are also presented. A non-iterative combined algorithm, MLE-HLS, based on MLE and HLS, is further proposed in this paper. The performance of all algorithms is analysed and compared in terms of accuracy in the localization of the position of the emitter and in terms of computational time. The analysis is also undertaken with three different sensor layouts since the positions of the sensors affect the localization; several source positions are also evaluated to make the comparison more robust. The analysis is carried out using theoretical time differences, as well as including errors due to the effect of digital sampling of the time variables. It is shown that the most balanced algorithm, yielding better results than the other algorithms in terms of accuracy and short computational time, is the combined MLE-HLS algorithm.
A Subnano-g Electrostatic Force-Rebalanced Flexure Accelerometer for Gravity Gradient Instruments
Authors: Yan Shitao , Xie Yafei , Zhang Mengqi , Deng Zhongguang , Tu Liangcheng
Published: November 2017

A subnano-g electrostatic force-rebalanced flexure accelerometer is designed for the rotating accelerometer gravity gradient instrument. This accelerometer has a large proof mass, which is supported inversely by two pairs of parallel leaf springs and is centered between two fixed capacitor plates. This novel design enables the proof mass to move exactly along the sensitive direction and exhibits a high rejection ratio at its cross-axis directions. Benefiting from large proof mass, high vacuum packaging, and air-tight sealing, the thermal Brownian noise of the accelerometer is lowered down to less than 0.2 ng / Hz with a quality factor of 15 and a natural resonant frequency of about 7.4 Hz . The accelerometer’s designed measurement range is about ±1 mg. Based on the correlation analysis between a commercial triaxial seismometer and our accelerometer, the demonstrated self-noise of our accelerometers is reduced to lower than 0.3 ng / Hz over the frequency ranging from 0.2 to 2 Hz, which meets the requirement of the rotating accelerometer gravity gradiometer.
Optimized Lateral Flow Immunoassay Reader for the Detection of Infectious Diseases in Developing Countries
Authors: Pilavaki Evdokia , Demosthenous Andreas
Published: November 2017

Detection and control of infectious diseases is a major problem, especially in developing countries. Lateral flow immunoassays can be used with great success for the detection of infectious diseases. However, for the quantification of their results an electronic reader is required. This paper presents an optimized handheld electronic reader for developing countries. It features a potentially low-cost, low-power, battery-operated device with no added optical accessories. The operation of this proof of concept device is based on measuring the reflected light from the lateral flow immunoassay and translating it into the concentration of the specific analyte of interest. Characterization of the surface of the lateral flow immunoassay has been performed in order to accurately model its response to the incident light. Ray trace simulations have been performed to optimize the system and achieve maximum sensitivity by placing all the components in optimum positions. A microcontroller enables all the signal processing to be performed on the device and a Bluetooth module allows transmission of the results wirelessly to a mobile phone app. Its performance has been validated using lateral flow immunoassays with influenza A nucleoprotein in the concentration range of 0.5 ng/mL to 200 ng/mL.
A Dual Band Frequency Reconfigurable Origami Magic Cube Antenna for Wireless Sensor Network Applications
Authors: Shah Syed , Lim Sungjoon
Published: November 2017

In this paper, a novel dual band frequency reconfigurable antenna using an origami magic cube is proposed for wireless sensor network (WSN) applications. The proposed origami antenna consists of a meandered monopole folded onto three sides of the magic cube. A microstrip open-ended stub is loaded on the meandered monopole. The proposed origami magic cube can be mechanically folded and unfolded. The proposed antenna operates at 1.57 GHZ and 2.4 GHz in the folded state. In the unfolded state, the proposed antenna operates at 900 MHz and 2.3 GHz. The resonant frequency of the second band can be tunable by varying the length and position of the open stub. The origami magic cube is built on paper. Its performance is numerically and experimentally demonstrated from S-parameters and radiation patterns. The measured 10 dB impedance bandwidth of the proposed origami antenna is 18% (900–1120 MHz) and 15% (2.1–2.45 GHz) for the unfolded state and 20% (1.3–1.6 GHz) and 14% (2.3–2.5 GHz) for the folded state. The measured peak gain at 900 MHz and 2.3 GHz are 1.1 dBi and 2.32 dBi, respectively, in the unfolded state. The measured peak gain at 1.5 GHz and 2.4 GHz are 3.28 dBi and 1.98 dBi, respectively, in the folded state.
Conflicts of Interest and Change in Original Intent: A Case Study of Vacant and Abandoned Homes Repurposed as Community Gardens in a Shrinking City, Daegu, South Korea
Authors: Lee Jin-Wook , Sung Jong-Sang
Published: November 2017

As part of an urban policy designed to revive South Korea’s shrinking cities, vacant residential structures are being demolished and the resulting empty plots transformed into public spaces. This study discursively examines this process, its stakeholders, and the sources of conflict among them in the neighborhood of Daebong 2 in Daegu, South Korea. Additionally, solutions for maintaining public interest are explored. Employees and members of relevant municipal authorities and non-profit organizations (NPOs), as well as town residents, were selected through purposive sampling for interviews. The data were then analyzed via open coding. The results reveal conflict between users and non-users in terms of the possession of public goods, as well as conflict between project executives in the creation process. We also found that spatial and policy characteristics are a particular source of conflict in dense, historic residential areas. To overcome problems caused by rivalry and discord, the following actions are required: a change in perspective among policy practitioners; a governance structure that consists of a public/private/community partnership; consensus among community members, and; equitable welfare through programs based on inclusivity and public interest.
Singapore as Science ’Scape and Ethnoscape
Authors: Cho Philip S. , Valler Dave
Published: November 2017

As a state project to locate the nation within the global knowledge economy, Singapore’s Technology Corridor has acted as a testbed for experimenting with different models of an international scientific community. Its landscape, from garden-style science parks modelled after Silicon Valley to monuments for multidisciplinary research such as One North, reflects the dynamic interaction among various political and ideological aspirations—ideoscapes—driving the built environment. Through both documentary research and semi-structured interviews of scientists and entrepreneurs, we examine how mostly foreign expat and some Singaporean researchers have experienced Singapore’s unique science ‘scape and adapted to a hybrid work and living environment. Testimony of the scientific sojourners of Singapore’s diverse ethnoscape—from China and India to Europe and the United States—reveal the blending of different styles of scientific work and the interconnected flows of intellectual labour.
Amphitropical disjunctions in New World Menthinae: Three Pliocene dispersals to South America following late Miocene dispersal to North America from the Old World
Authors: Drew Bryan T. , Liu Sitong , Bonifacino Jose M. , Sytsma Kenneth J.
Published: November 2017

PREMISE OF THE STUDY:

The subtribe Menthinae (Lamiaceae), with 35 genera and 750 species, is among the largest and most economically important subtribes within the mint family. Most genera of Menthinae are found exclusively in the New World, where the group has a virtually continuous distribution ranging from temperate North America to southern South America. In this study, we explored the presence, timing, and origin of amphitropical disjuncts within Menthinae.

METHODS:

Our analyses were based on a data set consisting of 89 taxa and the nuclear ribosomal DNA markers ITS and ETS. Phylogenetic relationships were determined under maximum likelihood and Bayesian criteria, divergence times were estimated with the program BEAST, and ancestral range estimated with BioGeoBEARS.

KEY RESULTS:

A North Atlantic Land Bridge migration event at about 10.6 Ma is inferred from western Eurasia to North America. New World Menthinae spread rapidly across North America, and then into Central and South America. Several of the large speciose genera are not monophyletic with nuclear rDNA, a finding mirrored with previous chloroplast DNA results. Three amphitropical disjunctions involving North and southern South America clades, one including a southeastern South American clade with several genera, were inferred to have occurred within the past 5 Myr.

CONCLUSIONS:

Although three New World Menthinae genera occur in both North and South America, none exhibit an amphitropical disjunction. However, three clades exhibit amphitropical disjunctions, all dating to the early Pliocene, and all involve jump dispersals to either southeastern or southwestern South America from southeastern North America.
The Broadband and Spectrally Resolved H-band Eclipse of KELT-1b and the Role of Surface Gravity in Stratospheric Inversions in Hot Jupiters
Authors: Beatty Thomas G. , Madhusudhan Nikku , Pogge Richard , Chung Sun Mi , Bierlya Allyson , Gaudi B. Scott , Latham David W.
Published: November 2017

We present a high-precision H-band emission spectrum of the transiting brown dwarf KELT-1b, which we spectrophotometrically observed during a single secondary eclipse using the LUCI1 multiobject spectrograph on the Large Binocular Telescope. Using a Gaussian-process regression model, we are able to clearly measure the broadband eclipse depth as ΔH = 1418 ± 94 ppm. We are also able to spectrally resolve the H band into five separate wave channels and measure the eclipse spectrum of KELT-1b at R ≈ 50 with an average precision of ±135 ppm. We find that the day side has an average brightness temperature of 3250 ± 50 K, with significant variation as a function of wavelength. Based on our observations and previous measurements of KELT-1b’s eclipse at other wavelengths, we find that KELT-1b’s day side appears identical to an isolated 3200 K brown dwarf, and our modeling of the atmospheric emission shows a monotonically decreasing temperature–pressure profile. This is in contrast to hot Jupiters with similar day-side brightness temperatures near 3000 K, all of which appear to be either isothermal or possess a stratospheric temperature inversion. We hypothesize that the lack of an inversion in KELT-1b is due to its high surface gravity, which we argue could be caused by the increased efficiency of cold-trap processes within its atmosphere.
“Orphan” γ-Ray Flares and Stationary Sheaths of Blazar Jets
Authors: MacDonald Nicholas R. , Jorstad Svetlana G. , Marscher Alan P.
Published: November 2017

Blazars exhibit flares across the entire electromagnetic spectrum. Many γ-ray flares are highly correlated with flares detected at longer wavelengths; however, a small subset appears to occur in isolation, with little or no correlated variability at longer wavelengths. These “orphan” γ-ray flares challenge current models of blazar variability, most of which are unable to reproduce this type of behavior. MacDonald et al. have developed the Ring of Fire model to explain the origin of orphan γ-ray flares from within blazar jets. In this model, electrons contained within a blob of plasma moving relativistically along the spine of the jet inverse-Compton scatter synchrotron photons emanating off of a ring of shocked sheath plasma that enshrouds the jet spine. As the blob propagates through the ring, the scattering of the ring photons by the blob electrons creates an orphan γ-ray flare. This model was successfully applied to modeling a prominent orphan γ-ray flare observed in the blazar PKS 1510−089. To further support the plausibility of this model, MacDonald et al. presented a stacked radio map of PKS 1510−089 containing the polarimetric signature of a sheath of plasma surrounding the spine of the jet. In this paper, we extend our modeling and stacking techniques to a larger sample of blazars: 3C 273, 4C 71.01, 3C 279, 1055+018, CTA 102, and 3C 345, the majority of which have exhibited orphan γ-ray flares. We find that the model can successfully reproduce these flares, while our stacked maps reveal the existence of jet sheaths within these blazars.
A Mote in Andromeda's Disk: A Misidentified Periodic AGN behind M31
Authors: Dorn-Wallenstein Trevor , Levesque Emily M. , Ruan John J.
Published: November 2017

We identify an object previously thought to be a star in the disk of M31, J0045+41, as a background

active galactic nucleus (AGN) seen through a low-absorption region of M31. We present moderate resolution spectroscopy of J0045+41 obtained using GMOS at Gemini-North. The spectrum contains features attributable to the host galaxy. We model the spectrum to estimate the AGN contribution, from which we estimate the luminosity and virial mass of the central engine. Residuals to our fit reveal a blueshifted component to the broad Hα and Hβ at a relative velocity of ∼4800 km s−1. We also detect Na i absorption in the Milky Way rest-frame. We search for evidence of periodicity using g-band photometry from the Palomar Transient Factory and find evidence for multiple periodicities ranging from ∼80–350 days. Two of the detected periods are in a 1:4 ratio, which is identical to the predictions of hydrodynamical simulations of binary supermassive black hole systems. If these signals arise due to such a system, J0045+41 is well within the gravitational wave regime. We calculate the time until inspiral due to gravitational radiation, assuming reasonable values of the mass ratio of the two black holes. We discuss the implications of our findings and forthcoming work to identify other such interlopers in the light of upcoming photometric surveys such as the Zwicky Transient Facility or the Large Synoptic Survey Telescope projects.
On the Time Variation of Dust Extinction and Gas Absorption for Type Ia Supernovae Observed through a Nonuniform Interstellar Medium
Authors: Huang X. , Aldering G. , Biederman M. , Herger B.
Published: November 2017

For Type Ia supernovae (SNe Ia) observed through a nonuniform interstellar medium (ISM) in its host galaxy, we investigate whether the nonuniformity can cause observable time variations in dust extinction and in gas absorption due to the expansion of the SN photosphere with time. We show that, owing to the steep spectral index of the ISM density power spectrum, sizable density fluctuation amplitudes at the length scale of typical ISM structures (

) will translate to much smaller fluctuations on the scales of an SN photosphere. Therefore, the typical amplitude of time variation due to a nonuniform ISM, of absorption equivalent widths, and of extinction, would be small. As a result, we conclude that nonuniform ISM density should not impact cosmology measurements based on SNe Ia. We apply our predictions based on the ISM density power-law power spectrum to the observations of two highly reddened SNe Ia, SN 2012cu and SN 2014J.
Demonstrating a New Census of Infrared Galaxies with ALMA (DANCING-ALMA). I. FIR Size and Luminosity Relation at z = 0–6 Revealed with 1034 ALMA Sources
Authors: Fujimoto Seiji , Ouchi Masami , Shibuya Takatoshi , Nagai Hiroshi
Published: November 2017

We present the large statistics of the galaxy effective radius R
e in the rest-frame far-infrared (FIR) wavelength

obtained from 1627 Atacama Large Millimeter/submillimeter Array (ALMA) 1 mm band maps that become public by 2017 July. Our ALMA sample consists of 1034 sources with the star formation rate

and the stellar mass

at z = 0–6. We homogeneously derive

and FIR luminosity L
FIR of our ALMA sources via the uv-visibility method with the exponential disk model, carefully evaluating selection and measurement incompletenesses by realistic Monte-Carlo simulations. We find that there is a positive correlation between

and L
FIR at the >99% significance level. The best-fit power-law function,

, provides

, and shows that

at a fixed L
FIR decreases toward high redshifts. The best-fit α and the redshift evolution of

are similar to those of R
e in the rest-frame UV (optical) wavelength

(

) revealed by Hubble Space Telescope (HST) studies. We identify that our ALMA sources have significant trends of

and

, which suggests that the dusty starbursts take place in compact regions. Moreover,

of our ALMA sources is comparable to

of quiescent galaxies at z ∼ 1–3 as a function of stellar mass, supporting the evolutionary connection between these two galaxy populations. We also investigate rest-frame UV and optical morphologies of our ALMA sources with deep HST images, and find that ∼30%–40% of our ALMA sources are classified as major mergers. This indicates that dusty starbursts are triggered by not only the major mergers but also the other mechanism(s).
An X-Ray Study of Two B+B Binaries: AH Cep and CW Cep
Authors: Ignace R. , Hole K. T. , Oskinova L. M. , Rotter J. P.
Published: November 2017

AH Cep and CW Cep are both early B-type binaries with short orbital periods of 1.8 days and 2.7 days, respectively. All four components are B0.5V types. The binaries are also double-lined spectroscopic and eclipsing. Consequently, solutions for orbital and stellar parameters make the pair of binaries ideal targets for a study of the colliding winds between two B stars. Chandra ACIS-I observations were obtained to determine X-ray luminosities. AH Cep was detected with an unabsorbed X-ray luminosity at a 90% confidence interval of

erg s−1, or

, relative to the combined Bolometric luminosities of the two components. While formally consistent with expectations for embedded wind shocks, or binary wind collision, the near-twin system of CW Cep was a surprising nondetection. For CW Cep, an upper limit was determined with

, again for the combined components. One difference between these two systems is that AH Cep is part of a multiple system. The X-rays from AH Cep may not arise from standard wind shocks nor wind collision, but perhaps instead from magnetism in any one of the four components of the system. The possibility could be tested by searching for cyclic X-ray variability in AH Cep on the short orbital period of the inner B stars.
Old but Still Warm: Far-UV Detection of PSR B0950+08*
Authors: Pavlov G. G. , Rangelov B. , Kargaltsev O. , Reisenegger A. , Guillot S. , Reyes C.
Published: November 2017

We report on a Hubble Space Telescope detection of the nearby, old pulsar B0950+08 (

pc, spin-down age 17.5 Myr) in two far-ultraviolet (FUV) bands. We measured the mean flux densities

nJy and 83 ± 14 nJy in the F125LP and F140LP filters (pivot wavelengths 1438 and 1528 Å). Using the FUV data together with previously obtained optical–UV data, we conclude that the optical–FUV spectrum consists of two components—a nonthermal (presumably magnetospheric) power-law spectrum (

) with slope

and a thermal spectrum emitted from the bulk of the neutron star (NS) surface with a temperature in the range of (1–3) × 105 K, depending on interstellar extinction and NS radius. These temperatures are much higher than predicted by NS cooling models for such an old pulsar, which means that some heating mechanisms operate in NSs. A plausible mechanism responsible for the high temperature of PSR B0950+08 is the interaction of vortex lines of the faster rotating neutron superfluid with the slower rotating normal matter in the inner NS crust (vortex creep heating).
A Multi-telescope Campaign on FRB 121102: Implications for the FRB Population
Authors: Law C. J. , Abruzzo M. W. , Bassa C. G. , Bower G. C. , Burke-Spolaor S. , Butler B. J. , Cantwell T. , Carey S. H. , Chatterjee S. , Cordes J. M. , Demorest P. , Dowell J. , Fender R. , Gourdji K. , Grainge K. , Hessels J. W. T. , Hickish J. , Kaspi V. M. , Lazio T. J. W. , McLaughlin M. A. , Michilli D. , Mooley K. , Perrott Y. C. , Ransom S. M. , Razavi-Ghods N. , Rupen M. , Scaife A. , Scott P. , Scholz P. , Seymour A. , Spitler L. G. , Stovall K. , Tendulkar S. P. , Titterington D. , Wharton R. S. , Williams P. K. G.
Published: November 2017

We present results of the coordinated observing campaign that made the first subarcsecond localization of a fast radio burst, FRB 121102. During this campaign, we made the first simultaneous detection of an FRB burst using multiple telescopes: the VLA at 3 GHz and the Arecibo Observatory at 1.4 GHz. Of the nine bursts detected by the Very Large Array at 3 GHz, four had simultaneous observing coverage at other observatories at frequencies from 70 MHz to 15 GHz. The one multi-observatory detection and three non-detections of bursts seen at 3 GHz confirm earlier results showing that burst spectra are not well modeled by a power law. We find that burst spectra are characterized by a ∼500 MHz envelope and apparent radio energy as high as 1040 erg. We measure significant changes in the apparent dispersion between bursts that can be attributed to frequency-dependent profiles or some other intrinsic burst structure that adds a systematic error to the estimate of dispersion measure by up to 1%. We use FRB 121102 as a prototype of the FRB class to estimate a volumetric birth rate of FRB sources

Mpc−3 yr−1, where N

r
 is the number of bursts per source over its lifetime. This rate is broadly consistent with models of FRBs from young pulsars or magnetars born in superluminous supernovae or long gamma-ray bursts if the typical FRB repeats on the order of thousands of times during its lifetime.