bluuebunny/crossref_metadata_2025 huggingface dataset:
Changes in body composition after glucocorticoid therapy in patients with systemic lupus erythematosus
Authors: Mok CC , To CH , Ma KM
Published: October 2008

The aim of this study was to evaluate the changes in body composition after glucocorticoid treatment in patients with systemic lupus erythematosus (SLE). Consecutive SLE patients were recruited for serial measurements (baseline, months 2 and 6) of bone mineral density (BMD) and body composition [bone mineral content (BMC), fat and lean mass] by dual energy X-ray absorptiometry scan after high-dose oral glucocorticoid therapy. Factors correlated with changes in body composition were evaluated. 29 SLE patients were studied (age 39.7 ± 11.5 years; 83% women with 29% postmenopausal; SLE duration 80.1 ± 80 months). Fourteen patients (48%) were glucocorticoid-naive. The mean maximum daily dosage of prednisolone was 32.9 ± 6.5 mg and the cumulative prednisolone dosage in 6 months was 2.7 ± 0.7 g. At 6 months, a significant drop in BMC of the trunk (−5.0 ± 2.2%; P = 0.04) and whole body (−1.2 ± 0.4%; P = 0.002) compared with baseline was observed, and so was the BMD of the hip (−1.7 ± 0.6%; P = 0.006) and whole body (−0.7 ± 0.3%; P = 0.01). A significant increase in the fat mass of the trunk (+14.5 ± 4.1%; P = 0.001) and limbs (+10.0 ± 3.2%; P = 0.004), but a non-significant drop in lean mass of the trunk (−3.3 ± 1.8%; P = 0.08) and limbs (−0.8 ± 2.4%; P = 0.75) also occurred. The changes in whole body BMC correlated significantly with age (ρ = −0.51; P = 0.02) and changes in total fat mass (ρ = 0.44; P = 0.02) but not with lean mass (ρ = −0.21; P = 0.27), gender, body mass index, smoking, prednisolone dosages or changes in BMD. In SLE patients, high-dose glucocorticoids lead to an early and rapid drop in bone mass, which is more serious in older patients and correlates with an increase in body fat.
Initial and accrued damage as predictors of mortality in Brazilian patients with systemic lupus erythematosus: a cohort study
Authors: Cardoso CRL , Signorelli FV , Papi JAS , Salles GF
Published: October 2008

The aim of this study was to investigate whether initial and accrued organ damage measured by Systemic Lupus International Collaborating Clinics (SLICC) Damage Index (SDI) predicts mortality in cohort of Brazilian patients with systemic lupus erythematosus (SLE). One hundred and five outpatients with SLE were enrolled from July 2000 to March 2001; their demographics, disease manifestations, interventions and quantified disease activity (SLEDAI) were obtained. SDI was measured at baseline and at the end of follow-up. Initial and accrued SDI prognostic values for mortality were investigated by multivariate Cox survival analysis and Kaplan-Meyer survival curves. After a median follow-up of 6.3 years, 19 patients died due to disease activity, end-organ failure, cardiovascular events, cancer and infection. Deceased patients had longer disease duration and greater initial and final SDI than survivors had. After adjustment for age, sex and disease duration, both initial and final SDI ≥ 3 points were independent predictors of mortality, with hazard ratios (HRs) of 3.0 (1.1–8.2) and 4.7 (1.6–14.5), respectively. Damage accrual during follow-up was the strongest predictor of death (HR: 5.1, 2.0–13.0). Renal and pulmonary damages were the main predictors of increased mortality risk. In conclusion, baseline and accrued damage increase mortality risk in Brazilian patients with SLE. Measures to prevent damage development and progression are urgent to reduce the mortality of patients with SLE.
The habit of holidays
Authors: Blichfeldt Bodil Stilling
Published: October 2008

Generally, people's decision-making processes leading to their going on holiday are defined as complex processes characterized by high degrees of uncertainty and risk; substantial expenditure; and elaborate pre-purchase information search. However, the series of qualitative interviews which this article reports suggest that it is too simplistic to define up-front holiday decision-making processes as extensive problem-solving. The interviews reveal three patterns of holiday decision-making among the Danish informants. For those informants who view holidays away from home as central to their lives, decision-making processes are `habitualized'. Those informants for whom holidays are of lesser importance rely on ad-hoc, low involvement, decision-making. Only the final group of informants, who have recently started to go on new types of holidays, engage in extensive problem-solving. Drawing on Berger and Luckman's discussion on institutionalization, this article explores why extensive problem-solving is only one of the different decision-making processes that people rely on when planning their holidays.
Depathologizing the tourist syndrome
Authors: Heimtun Bente
Published: October 2008

The intention of this article is to discuss and contrast two central aspects of a published interview with Zygmunt Bauman addressing the nature of `the tourist syndrome' (Franklin, 2003). First, the tourist syndrome is a metaphor for contemporary living in liquid modernity and second, tourism is referred to as `a substitute satisfaction of a genuine need' (Franklin, 2003: 214). The interview presents a critical and somewhat sceptical perspective on tourism and social life, in which the tourist syndrome is labelled a `peg community' and the tourism industry characterized as an insatiable seducer. Based on the experiences of Norwegian midlife single women, a more positive notion is suggested. Although most of the midlife single women do not seek difference as tourists, the meaning of tourism is not superficial and/or contrived. It is rather a space for bonding with significant others and about social integration in everyday life.
Poverty, negative duties and the global institutional order
Authors: Reitberger Magnus
Published: October 2008

Do we violate human rights when we cooperate with and impose a global institutional order that engenders extreme poverty? Thomas Pogge argues that by shaping and enforcing the social conditions that foreseeably and avoidably cause global poverty we are violating the negative duty not to cooperate in the imposition of a coercive institutional order that avoidably leaves human rights unfulfilled. This article argues that Pogge's argument fails to distinguish between harms caused by the global institutions themselves and harms caused by the domestic policies of particular states and collective action problems for which collective responsibility cannot be assigned. The article also argues that his position relies on questionable factual and theoretical claims about the impact of global institutions on poverty, and about the benefits and harms of certain features of these institutions. Participation in, and benefit from, global institutions is unlikely to constitute a violation of our negative duties towards the poor.
On the meta-ethical status of constructivism: reflections on G.A. Cohen's `Facts and Principles'
Authors: Ronzoni Miriam , Valentini Laura
Published: October 2008

In his article `Facts and Principles', G.A. Cohen attempts to refute constructivist approaches to justification by showing that, contrary to what their proponents claim, fundamental normative principles are fact- insensitive. We argue that Cohen's `fact-insensitivity thesis' does not provide a successful refutation of constructivism because it pertains to an area of meta-ethics which differs from the one tackled by constructivists. While Cohen's thesis concerns the logical structure of normative principles, constructivists ask how normative principles should be justified. In particular, their claim that justified fundamental normative principles are fact-sensitive follows from a commitment to agnosticism about the existence of objective moral facts. We therefore conclude that, in order to refute constructivism, Cohen would have to address questions of justification, and take a stand on those long-standing meta-ethical debates about the ontological status of moral notions (for example, realism versus anti-realism) with respect to which he himself wants to remain agnostic.
Toward a theory of the basic minimum
Authors: Dorsey Dale
Published: October 2008

Many have thought that an important feature of any just society is the establishment and maintenance of a suitable basic minimum: some set of welfare achievements, resources, capabilities, and so on that are guaranteed to all. However, if a basic minimum is a plausible requirement of justice, we must have a theory — a theory of what, precisely, the state owes in terms of these basic needs or achievements and what, precisely, is the proper structure of the obligation to provide them. In Section 1, I will critically examine one recent influential account of the basic minimum: Martha Nussbaum's `human capabilities approach'. I argue that Nussbaum's account has several structural features, few of which are independently plausible, and which create insuperable difficulties when viewed in combination. The failure of Nussbaum's account is instructive, however. It provides motivation for the positive account I sketch in Section 2.
Reply: clubbish justice
Authors: Spiekermann Kai
Published: October 2008

Replying to my earlier article `Translucency, Assortation, and Information Pooling: How Groups Solve Social Dilemmas', Robert Goodin examines the normative implications of the rule `cooperate with those whose inclusion benefits the larger scheme of cooperation', and gives several reasons for why the conversion of justice into a club good is normatively unappealing. This reply to Goodin discusses whether the rule leads to an exclusion of poor agents, whether a group should hire agents to detect free-riders, and how a group should deal with naive cooperators. The rule can be defended as an enforcement mechanism in some cases, but it is normatively unappealing as a theory of justice.
Microstructure and strength modelling of Al–Cu–Mg alloys during non-isothermal treatments: Part 1 – Controlled heating and cooling
Authors: Khan I. N. , Starink M. J.
Published: October 2008

A model is developed to predict the precipitation kinetics and strengthening in Al–Cu–Mg alloys during non-isothermal treatments consisting of controlled heating and cooling. The prediction of the precipitation kinetics is based on the Kampmann and Wagner model. The precipitation strengthening by the shearable Cu–Mg co-clusters is modelled on the basis of the modulus strengthening mechanism and the strengthening by the non-shearable S phase precipitates is based on the Orowan looping mechanism. The model predictions are verified by comparing with hardness, transmission electron microscopy and differential scanning calorimetry data on 2024-T351 aluminium alloys. The microstructural development and strength predictions of the model are generally in close agreement with the experimental data.
Microstructure and strength modelling of Al–Cu–Mg alloys during non-isothermal treatments: Part 2 – Welds
Authors: Khan I. N. , Starink M. J. , Sinclair I. , Wang S. C.
Published: October 2008

The present work applies a model for microstructural evolution in the solid state and Al–Cu–Mg alloys and expands it in a computationally efficient way to include solid–liquid reactions in fusion welds. The model is used to predict local strength and hardness of the welds, using a formulation that incorporates hardening due to two types of precipitates, i.e. Cu–Mg co-clusters and the S phase precipitates. The model predictions are compared with hardness, differential scanning calorimetry and transmission electron microscopy data for a fusion welded 2024-T351 aluminium alloy. The model predicts solid state reactions and solid–liquid reactions including co-cluster dissolution, S phase formation, growth, coarsening and dissolution, co-cluster reformation on cooling, and solute partitioning on resolidification. The model predictions are in good agreement with the experimental results and illustrate the dominant role that (sub-)nanoscale co-clusters play in strengthening of welds. The yield strength of as welded material tested normal to the weld is mainly due to the co-clusters.
Microstructural path model and strain dependence of recrystallisation in commercial aluminium
Authors: Vandermeer R. A. , Wu G. L. , Juul Jensen D.
Published: October 2008

The isothermal recrystallisation of commercial purity aluminium alloy AA1200 cold deformed to either a true strain of 2 (86·5% reduction in thickness) or 4 (98·2% reduction in thickness) was studied phenomenologically in each material by means of quantitative microscopy. The microstructural path descriptors, VV, the volume fraction recrystallised, and SV, the interfacial area density separating recrystallised grains from deformed grains were measured stereologically by electron backscatter diffraction and microstructural path model parameters were deduced for each strain. The effects of strain were delineated and compared with the results of recrystallisation in a slightly different commercial aluminium alloy AA1050 deformed to a true strain of 2·3.
Role of second phase particles on microstructure and texture evolution of ARB processed aluminium sheets
Authors: Pirgazi H. , Akbarzadeh A.
Published: October 2008

The accumulative roll bonding (ARB) process was carried out on a high purity alloy (AA1100) and a particle containing aluminium alloy (AA3003) for up to eight cycles. The electron backscattered diffraction (EBSD) method was utilised to investigate the microstructural and microtextural evolution in ARB processed sheets. The results indicate that the lack of second phase particles in pure aluminium hinders grain refinement and leads to the formation of unrefined bands, which results in the increase of the overall texture intensity and the development of a strong texture. A submicrometre grain structure in this alloy develops at the final stages of the process. It was also found that the presence of second phase particles in AA3003 alloy prevents the development of such unrefined bands and improves grain refinement during the ARB process, which results in a more homogenous microstructure of ultrafine grains.
Thermal contact at solder/substrate interfaces during solidification
Authors: Nyamannavar S. , Prabhu K. N.
Published: October 2008

Heat flux transients at the solder/substrate interface during the solidification of Sn–37Pb and Sn–3·5Ag solder alloys against metallic substrates were estimated by the lumped heat capacitance model and the contact condition was assessed by scanning electronic microscopy (SEM). Copper substrates yielded maximum contact heat flux followed by brass and aluminium substrates. The SEM study in the solder/substrate interfacial region revealed the existence of a clear gap with the aluminium substrate. A conforming contact was obtained with copper and brass substrates.
Comparison of residual stresses in Ti–6Al–4V and Ti–6Al–2Sn–4Zr–2Mo linear friction welds
Authors: Frankel P. , Preuss M. , Steuwer A. , Withers P. J. , Bray S.
Published: October 2008

In this paper, the levels of residual stress in the vicinity of linear friction welds in Ti–6Al–4V (Ti-64), a conventional α– β titanium alloy, and Ti–6Al–2Sn–4Zr–2Mo (Ti-6242), a near α titanium alloy with higher temperature capability, are mapped and contrasted. The alloys have significantly different high temperature properties and the aim of this work was to investigate how this might affect their propensity to accumulate weld residual stresses and their response to post-weld heat treatment. Measurements are reported using high energy synchrotron X-ray diffraction and the results are compared to those made destructively using the contour method. The strain free lattice plane d0 variation across the weld has been evaluated using the biaxial sin2Ψ technique with laboratory X-rays. It was found that failure to account for the d0 variation across the weld line would have led to large errors in the peak tensile stresses. Contour method measurements show fairly good correlation with the diffraction results, although the stresses are underestimated. Possible reasons for the discrepancy are discussed. The peak tensile residual stresses introduced by the welding process were found to be greater for Ti-6242 (∼750 MPa) than for Ti-64 (∼650 MPa). Consistent with the higher temperature capability of the alloy, higher temperature post-weld heat treatments have been found to be necessary to relieve the stresses in the near α titanium alloy compared to the α+ β titanium alloy.
Simulation and application of dynamic heat transfer model for improvement of continuous casting process
Authors: Ma J. C. , Xie Z. , Ci Y. , Jia G. L.
Published: October 2008

Numerical simulation is being increasingly used to improve the existing cooling systems. In order to attain highest quality strand, a two-dimensional dynamic mathematical heat transfer model of billet continuous casting of low carbon steel has been presented. This model can be used to compute the billet temperature distribution and shell thickness, especially it can be used to simulate the solidification process which is caused by frequently variational casting conditions. The fluctuation of measured temperature has been reduced to <10°C with thermal imaging system. The online model can monitor surface temperature and shell thickness in the casting process. So it provides the possibility for the online process control. For the validation of the dynamic model, a lot of billet surface temperature and shell thickness measurement were carried out on an actual casting machine. Finally, the dynamic model has been used for adjusting the operating parameters to improve the casting speed.
Zebrafish runx1 promoter-EGFP transgenics mark discrete sites of definitive blood progenitors
Authors: Yi Ni Lam Enid , Chau Jackie Y. M. , Kalev-Zylinska Maggie L. , Fountaine Timothy M. , Mead R. Scott , Hall Christopher J. , Crosier Philip S. , Crosier Kathryn E. , Vega Flores Maria
Published: October 2008

The transcription factor Runx1 is essential for the development of definitive hematopoietic stem cells (HSCs) during vertebrate embryogenesis and is transcribed from 2 promoters, P1 and P2, generating 2 major Runx1 isoforms. We have created 2 stable runx1 promoter zebrafish-transgenic lines that provide insight into the roles of the P1 and P2 isoforms during the establishment of definitive hematopoiesis. The Tg(runx1P1:EGFP) line displays fluorescence in the posterior blood island, where definitive erythromyeloid progenitors develop. The Tg(runx1P2:EGFP) line marks definitive HSCs in the aorta-gonad-mesonephros, with enhanced green fluorescent protein–labeled cells later populating the pronephros and thymus. This suggests that a function of runx1 promoter switching is associated with the establishment of discrete definitive blood progenitor compartments. These runx1 promoter–transgenic lines are novel tools for the study of Runx1 regulation and function in normal and malignant hematopoiesis. The ability to visualize and isolate fluorescently labeled HSCs should contribute to further elucidating the complex regulation of HSC development.
A new method to determine tissue specific tissue factor thrombomodulin activities: endotoxin and particulate air pollution induced disbalance
Authors: Frederix Kim , Kooter Ingeborg M , van Oerle René , Fens Diane , Hamulyak Karly , Gerlofs-Nijland Miriam E , ten Cate Hugo , Spronk Henri MH
Published: October 2008

Background

Increase in tissue factor (TF) and loss in thrombomodulin (TM) antigen levels has been described in various inflammatory disorders. The functional consequences of such changes in antigen concentrations in the coagulation balance are, however, not known. This study was designed to assess the consequences of inflammation-driven organ specific functional properties of the procoagulant response.

Methods

Tissue specific procoagulant activity was assessed by adding tissue homogenate to normal human pool plasma and recording of the thrombin generation curve. The new technique was subsequently applied on two inflammation driven animal models: 1) mouse lipopolysaccharide (LPS) induced endotoxemia and 2) spontaneously hypertensive rats exposed to environmental air pollution (particulate matter (PM).

Results

Addition of lung tissue from untreated animals to human plasma suppressed the endogenous thrombin potential (ETP) (175 ± 61 vs. 1437 ± 112 nM.min for control). This inhibitory effect was due to TM, because a) it was absent in protein C deficient plasma and b) lungs from TMpro/pro mice allowed full thrombin generation (ETP: 1686 ± 209 nM.min). The inhibitory effect of TM was lost after LPS administration to mice, which induced TF activity in lungs of C57Bl/6 mice as well as increased the ETP (941 ± 523 vs. 194 ± 159 nM.min for control). Another pro-inflammatory stimulus, PM dose-dependently increased TF in the lungs of spontaneously hypertensive rats at 4 and 48 hours after PM exposure. The ETP increased up to 48 hours at the highest concentration of PM (1441 ± 289 nM.min vs. saline: 164 ± 64 nM.min, p < 0.0001), suggesting a concentration- and time dependent reduction in TM activity.

Conclusion

Inflammation associated procoagulant effects in tissues are dependent on variations in activity of the TF-TM balance. The application of these novel organ specific functional assays is a useful tool to monitor inflammation-driven shifts in the coagulation balance within animal or human tissues.
The Military Lunatic Hospital
Authors: Robertson C. Lockhart
Published: October 2008

In a former number of this Journal (No. 11) attention was drawn in a leading article to the breaking up of the Military Lunatic Hospital at Yarmouth, Norfolk, and in the next number I was led to make one or two remarks on the question, actuated by a natural sympathy with the present sad state of my former patients.
Cloning and Expression of kiss2 in the Zebrafish and Medaka
Authors: Kitahashi Takashi , Ogawa Satoshi , Parhar Ishwar S.
Published: October 2008

Newly discovered kisspeptin (metastin), encoded by the Kiss1/KISS1 gene, is considered as a major gatekeeper of puberty through the regulation of GnRH. In the present study, we cloned a novel kisspeptin gene (kiss2) in the zebrafish Danio rerio and the medaka Oryzias latipes, which encodes a sequence of 125 and 115 amino acids, respectively, and its core sequence (FNLNPFGLRF, F-F form) is different from the previously characterized kiss1 (YNLNSFGLRY, Y-Y form). Our in silico data mining shows kiss1 and kiss2 are highly conserved across nonmammalian vertebrate species, and we have identified two putative kisspeptins in the platypus and three forms in Xenopus. In the brain of zebrafish and medaka, in situ hybridization and laser capture microdissection coupled with real-time PCR showed kiss1 mRNA expression in the ventromedial habenula and the periventricular hypothalamic nucleus. The kiss2 mRNA expression was observed in the posterior tuberal nucleus and the periventricular hypothalamic nucleus. Quantitative real-time PCR analysis during zebrafish development showed a significant increase in zebrafish kiss1, kiss2 (P < 0.002), gnrh2, and gnrh3 (P < 0.001) mRNA levels at the start of the pubertal phase and remained high in adulthood. In sexually mature female zebrafish, Kiss2 but not Kiss1 administration significantly increased FSH-β (2.7-fold, P < 0.05) and LH-β (8-fold, P < 0.01) mRNA levels in the pituitary. These results suggest that the habenular Kiss1 and the hypothalamic Kiss2 are potential regulators of reproduction including puberty and that Kiss2 is the predominant regulator of gonadotropin synthesis in fish.

Habenular kisspeptin-1 (Kiss1) and the novel hypothalamic Kiss2 are potential regulators of puberty. Kiss2 is the predominant regulator of gonadotropin synthesis in fish.
New coumarin-based anti-inflammatory drug: putative antagonist of the integrins αLβ2 and αMβ2
Authors: Bucolo Claudio , Maltese Adriana , Maugeri Francesco , Ward Keith W , Baiula Monica , Spartà Antonino , Spampinato Santi
Published: October 2008

This study was conducted to investigate putative antagonism of integrin receptors αMβ2 and αLβ2 by a novel coumarin derivative (BOL-303225-A), its efficacy in-vivo after retinal ischaemia-reperfusion injury, and its bioavailability in rat plasma. A cellular adhesion assay in Jurkat and U937 cells, and a flow cytometry assay with an antibody against the β2 subunit were conducted. BOL-303225-A bioavailability in rat plasma and the retinal levels of myeloperoxidase (MPO) after ischaemia- reperfusion injury were evaluated after oral administration (10 mg kg−1). In-vitro cell viability assays revealed no cytotoxicity for BOL-303225-A over a wide dose range, and IC50 values of 32.3 ± 1.5 μM and 84.95 ± 2.3 μM were found for Jurkat and U937 cells, respectively. The drug showed specific binding to the αMβ2 and αLβ2 integrin receptors expressed by U937 and Jurkat cells, respectively, producing a fluorescence shift towards lower values in a concentration-dependent manner. The pharmacokinetic profile of BOL-303225-A exhibited rapid absorption following oral administration in the rat. A significant reduction of retinal MPO levels was observed in drug-treated rats. This study demonstrated that BOL-303225-A acts as an antagonist of the integrin αLβ2 and αMβ2 receptors, suggesting that this drug could be used for ocular diseases such as diabetic retinopathy.
Wnt signaling determines ventral spinal cord cell fates in a time-dependent manner
Authors: Yu Weiying , McDonnell Kristen , Taketo Makoto M. , Bai C. Brian
Published: October 2008

The identity of distinct cell types in the ventral neural tube is generally believed to be specified by sonic hedgehog (Shh) in a concentration-dependent manner. However, recent studies have questioned whether Shh is the sole signaling molecule determining ventral neuronal cell fates. Here we provide evidence that canonical Wnt signaling is involved in the generation of different cell types in the ventral spinal cord. We show that Wnt signaling is active in the mouse ventral spinal cord at the time when ventral cell types are specified. Furthermore, using an approach that stabilizes β-catenin protein in small patches of ventral spinal cord cells at different stages, we show that Wnt signaling activates different subsets of target genes depending on the time when Wnt signaling is amplified. Moreover, disruption of Wnt signaling results in the expansion of ventrally located progenitors. Finally,we show genetically that Wnt signaling interacts with Hh signaling at least in part through regulating the transcription of Gli3. Our results reveal a novel mechanism by which ventral patterning is achieved through a coordination of Wnt and Shh signaling.
BMP signaling negatively regulates bone mass through sclerostin by inhibiting the canonical Wnt pathway
Authors: Kamiya Nobuhiro , Ye Ling , Kobayashi Tatsuya , Mochida Yoshiyuki , Yamauchi Mitsuo , Kronenberg Henry M. , Feng Jian Q. , Mishina Yuji
Published: October 2008

Bone morphogenetic proteins (BMPs) are known to induce ectopic bone. However, it is largely unknown how BMP signaling in osteoblasts directly regulates endogenous bone. This study investigated the mechanism by which BMP signaling through the type IA receptor (BMPR1A) regulates endogenous bone mass using an inducible Cre-loxP system. When BMPR1A in osteoblasts was conditionally disrupted during embryonic bone development, bone mass surprisingly was increased with upregulation of canonical Wnt signaling. Although levels of bone formation markers were modestly reduced, levels of resorption markers representing osteoclastogenesis were severely reduced,resulting in a net increase in bone mass. The reduction of osteoclastogenesis was primarily caused by Bmpr1a-deficiency in osteoblasts, at least through the RANKL-OPG pathway. Sclerostin (Sost) expression was downregulated by about 90% and SOST protein was undetectable in osteoblasts and osteocytes, whereas the Wnt signaling was upregulated. Treatment of Bmpr1a-deficient calvariae with sclerostin repressed the Wnt signaling and restored normal bone morphology. By gain of Smad-dependent BMPR1A signaling in mice, Sost expression was upregulated and osteoclastogenesis was increased. Finally, the Bmpr1a-deficient bone phenotype was rescued by enhancing BMPR1A signaling, with restoration of osteoclastogenesis. These findings demonstrate that BMPR1A signaling in osteoblasts restrain endogenous bone mass directly by upregulating osteoclastogenesis through the RANKL-OPG pathway, or indirectly by downregulating canonical Wnt signaling through sclerostin, a Wnt inhibitor and a bone mass mediator.
The hydrodynamics of ribbon-fin propulsion during impulsive motion
Authors: Shirgaonkar Anup A. , Curet Oscar M. , Patankar Neelesh A. , MacIver Malcolm A.
Published: October 2008

Weakly electric fish are extraordinarily maneuverable swimmers, able to swim as easily forward as backward and rapidly switch swim direction, among other maneuvers. The primary propulsor of gymnotid electric fish is an elongated ribbon-like anal fin. To understand the mechanical basis of their maneuverability, we examine the hydrodynamics of a non-translating ribbon fin in stationary water using computational fluid dynamics and digital particle image velocimetry (DPIV) of the flow fields around a robotic ribbon fin. Computed forces are compared with drag measurements from towing a cast of the fish and with thrust estimates for measured swim-direction reversals. We idealize the movement of the fin as a traveling sinusoidal wave, and derive scaling relationships for how thrust varies with the wavelength, frequency,amplitude of the traveling wave and fin height. We compare these scaling relationships with prior theoretical work. The primary mechanism of thrust production is the generation of a streamwise central jet and the associated attached vortex rings. Under certain traveling wave regimes, the ribbon fin also generates a heave force, which pushes the body up in the body-fixed frame. In one such regime, we show that as the number of waves along the fin decreases to approximately two-thirds, the heave force surpasses the surge force. This switch from undulatory parallel thrust to oscillatory normal thrust may be important in understanding how the orientation of median fins may vary with fin length and number of waves along them. Our results will be useful for understanding the neural basis of control in the weakly electric knifefish as well as for engineering bio-inspired vehicles with undulatory thrusters.
DrosophilaABC transporter mutantswhite,brownandscarlethave altered contents and distribution of biogenic amines in the brain
Authors: Borycz J. , Borycz J. A. , Kubów A. , Lloyd V. , Meinertzhagen I. A.
Published: October 2008

Monoamines such as dopamine, histamine and serotonin (5-HT) are widely distributed throughout the brain of the fruit fly Drosophila melanogaster, where many of their actions have been investigated. For example, histamine is released from photoreceptor synapses in the lamina neuropile of the visual system. Mutations of the genes white, an important eye pigmentation marker in fly genetics that encodes an ABC transporter, and its binding partner brown, cause neural phenotypes not readily reconciled solely with actions in eye pigmentation. We find that flies mutant for these genes, and another binding partner, scarlet, have about half the wild-type amount of histamine in the head, as well as reduced 5-HT and dopamine. These differences parallel reductions in immunoreactivity to the corresponding biogenic amines. They also correlate with the amine content of fractions after differential centrifugation of head homogenates. Thus, most of the amine is found in the vesicle-rich fraction of wild-type head homogenates, whereas it is found in the supernatant fractions from white, brown and scarlet flies. White co-expresses in lamina epithelial glia with Ebony, which conjugates histamine to β-alanine. Histamine is then released when the conjugate is hydrolyzed in photoreceptors, by Tan. Mutant white ameliorates the effects of tan on head histamine whereas it exacerbates the effects of ebony. Our results are consistent with the proposal that histamine uptake by the epithelial glia may be white dependent. Behavioral abnormalities in white, brown and scarlet mutants could arise because aminergic neurons in the Drosophila brain have reduced amine for release.
Regression of Kaposi's sarcoma lesions following highly active antiretroviral therapy in an HIV-infected patient
Authors: Kumarasamy N , Venkatesh K K , Devaleenol B , Poongulali S , Ahilasamy N
Published: October 2008

This case report documents that highly active antiretroviral therapy (HAART) can lead to the regression of Kaposi's sarcoma (KS) lesions in the auditory canal of an HIV-infected male from Chennai, India. In resource-limited settings where administering anti-KS chemotherapeutic agents may not be feasible, HAART alone can be an option in HIV-infected individuals with KS.
Thought processes associated with reluctance in gay men to be tested for HIV
Authors: Gold R S , Karantzas G
Published: October 2008

This study explored the thought processes that are associated with reluctance in gay men to be tested for HIV antibodies. The sample comprised 97 men who had not been tested for at least four years; 69 had never been tested. They were asked to imagine that someone had suggested that they be tested very soon and to identify, from the list provided, any negative thoughts prompted by this suggestion. The most commonly reported thoughts were that testing was unnecessary because risks had not been taken, that it was unnecessary because there were no symptoms, and that there was no urgency to be tested. Data were explored by means of factor analysis and comparisons across subgroups differing in risk level. The results are interpreted as indicating the use of rationalizations to buttress a decision not to be tested, the powerful influence on HIV decision-making exerted by salient perceptible features, and the ‘status quo bias’. Techniques that could be used to encourage testing in gay men are discussed.
Home telecare technologies for the elderly
Authors: Botsis Taxiarchis , Demiris George , Pedersen Steinar , Hartvigsen Gunnar
Published: October 2008

There are many home telecare technologies which have been developed specifically for chronic diseases and there are some more generic technologies that could be used as well. For home telecare, the equipment must be certified, the operational routines must be reformed, the infrastructure must be in place, the market must be prepared, the health authorities must be convinced that the system will work and the cost-effectiveness must be evaluated. Organizational and societal changes, such as cost reduction policies and an aging population, are the main driving forces for the development of home telecare, especially for elderly patients. At the moment there is no holistic model for scientific evaluation from different perspectives (e.g. clinical, legal, technical). We suggest that more research on home telecare and its effects needs to be conducted, in order to provide evidence for optimizing the use of this promising technique.
Telepathology training in a master of cytology degree course
Authors: Giansanti Daniele , Castrichella Livia , Giovagnoli Maria Rosaria
Published: October 2008

We have investigated the changes in the workflow of the cytologist due to the introduction of telepathology. These changes occur in two stages. The first is the use of telepathology as a support methodology using external providers (i.e. outside the hospital) to digitize the slides. The second is the use of telepathology in routine laboratory operations using an internal scanner to digitize the slides. To improve courses in the Master of Cytology programme at the University of La Sapienza, new learning modules were designed, which were made available via the wide area computer network to familiarize students with the new technologies. The new methodologies had three benefits. The first was the high level of knowledge for the student. The second was the cost advantage to the student, who did not need to study in a medical laboratory to participate in the teaching. The third was the cost advantage to the hospital: as laboratories become freed from academic work, they become more available for clinical use.
A novel, user-friendly step counter for home telemonitoring of physical activity
Authors: Giansanti Daniele , Maccioni Giovanni , Macellari Velio , Mattei Eugenio , Triventi Michele , Censi Federica , Calcagnini Giovanni , Bartolini Pietro
Published: October 2008

Step counting is an important index of motion in telemonitoring. We have developed a wearable system based on a device with a force-sensing resistor. This is affixed at the calf gastrocnemius level to monitor the muscular expansion related to the gait. The gastrocnemius expansion measurement unit (GEMU) was tested on three subjects at Level 2 of the Tinetti test of unbalance, who performed five repetitions of 100 steps at two different speeds (normal and slow). The mean error was less than 0.5%. The GEMU also performed better than an accelerometer unit, which is normally considered to be the best solution for this disability. The system can be integrated into a routine home-care application based on a GSM home-care unit.
The role of technology in video-mediated consensus meetings
Authors: Groth Kristina , Olin Kemal , Gran Ola , Permert Johan
Published: October 2008

We have studied the use of video-mediated technology to facilitate multidisciplinary meetings where consensus is reached about the diagnosis and treatment of patients. The meetings involved 2–5 sites, with 1–20 participants from each site, and concerned patients with severe diseases in the upper abdomen. During a period of about one year we conducted observations and interviews at the Karolinska hospital and some of the local hospitals. Eight video-mediated consensus meetings were video-recorded and transcribed. The audio and the radiology images presented during the meetings were considered to be most important; video was not critical for the consensus meeting itself, but contributed to a feeling of awareness and social presence. We conclude that video-mediated consensus meetings are affected by the technology used, and certain changes in the technology or environment could improve the meetings. Without the technology, such meetings would lead to more travelling for the patient and for the medical staff involved.
A method of extracting the number of trial participants from abstracts describing randomized controlled trials
Authors: Hansen Marie J , Rasmussen Nana Ø , Chung Grace
Published: October 2008

We have developed a method for extracting the number of trial participants from abstracts describing randomized controlled trials (RCTs); the number of trial participants may be an indication of the reliability of the trial. The method depends on statistical natural language processing. The number of interest was determined by a binary supervised classification based on a support vector machine algorithm. The method was trialled on 223 abstracts in which the number of trial participants was identified manually to act as a gold standard. Automatic extraction resulted in 2 false-positive and 19 false-negative classifications. The algorithm was capable of extracting the number of trial participants with an accuracy of 97% and an F-measure of 0.84. The algorithm may improve the selection of relevant articles in regard to question-answering, and hence may assist in decision-making.
Standardization, innovation and deployment of electronic referral software in Norway
Authors: Heimly Vigdis
Published: October 2008

The introduction of electronic referrals in Norway started in 1996, when the first standard for an electronic referral was developed. Subsequent implementations of electronic health record (EHR) systems have been tested by a national testing and approval service. Almost all the Norwegian EHR systems comply with the recommended standards, but the volume of electronic referrals remains low: only 8200 referrals were sent electronically in September 2007. In comparison, 125,000 electronic discharge summaries were sent during the same month. There are four elements that need to be in place before smooth communication can occur between the EHRs of the hospitals and the general practitioners. First, the regional health authorities must have a strategy for electronic cooperation. Second, various technical problems must be solved. Third, certain organizational problems must be addressed. Finally, electronic referrals should be used to support enhanced work processes and joint reception for referrals should be considered at larger hospitals.
An Active Grid infrastructure for elderly care
Authors: Hoang Doan , Lawrence Elaine
Published: October 2008

We propose a technique in which elderly people can be monitored non-intrusively. The information is kept in an ‘active’ health record which becomes alive when attention or action is necessary concerning the condition of the elderly person. The proposed system consists of three main components: a sensor/actor loop, sensor records and associated active services, and a Grid middleware platform. Information is captured in realtime within a collaborative health-care Grid. The Grid connects elderly people, caregivers and medical service providers in ways that reduce unnecessary calls on expensive medical services through an intermediate local service centre (which can be virtual) assisted with Internet communications and monitoring technologies. The proposal should support preventive health-care programmes for reducing the cost of caring for the elderly.
An exploratory study of disease surveillance systems in Norway
Authors: Johansen Monika A , Scholl Jeremiah , Aronsen Gudleif , Hartvigsen Gunnar , Bellika Johan G
Published: October 2008

We conducted a qualitative study of the system for contagious disease surveillance in Norway. Semi-structured interviews were held with five general practitioners (GPs), including one person responsible for informing GPs in their region about potentially serious disease outbreaks. The interviews suggested that the existing system had several limitations, making it of little relevance to local epidemics or daily medical practice. Specifically, it was difficult and time-consuming for physicians to locate relevant information, and there was a substantial delay between reported diagnoses and eventual feedback about outbreaks. This resulted in information that was too old to be of value. The interviews also investigated design matters related to future realtime disease surveillance systems. The GPs expressed interest in a distributed system for realtime extraction and presentation of data from electronic record systems. They required that any such system be customizable to the specific needs of the doctor in order to be relevant in day-to-day practice, and that correct interpretation of data would be possible in the minimum of time.
Awareness modelling in collaborative mobile e-health
Authors: Ray Pradeep , Parameswaran Nandan , Chan Victor , Yu Weider
Published: October 2008

E-health based on mobile wireless networks is called mobile e-health. Mobile e-health can facilitate computer-supported cooperative work, which encompasses tasks from email and instant messaging to wireless information sharing through broadband and telecommunication networks. In the development of cooperative management systems, the concept of ‘awareness level’ has been introduced. This is based on factors such as the location and actions of the user involved. Mobile e-health can help to achieve cooperation by providing the right awareness levels at the right time. The improved awareness levels allow health professionals to provide patient care with better quality and efficiency. Mobile e-health systems have the potential to take over the mundane tasks of the doctor so that better quality health services can be provided.
Identity-management factors in e-health and telemedicine applications
Authors: Savastano Mario , Hovsto Asbjorn , Pharow Peter , Blobel Bernd
Published: October 2008

Reliable identification is essential in e-health and telemedicine applications. This necessitates a secure and trustworthy method of communication and collaboration between parties, which depends on common acceptance. This in turn is related to privacy and ethical matters. Different technologies, including biometrics and RFID, allow high levels of security and safety in identifying both human beings and goods. However, the diffusion of standards relating to identity management in e-health is far from satisfactory. In order to support standardization in e-health, the European Commission funded the BioHealth project. This project has proved to be useful in promoting standards and creating awareness among the stakeholders.
Strategies to reduce interruptions from mobile communication systems in surgical wards
Authors: Solvoll Terje , Scholl Jeremiah
Published: October 2008

We conducted interviews with two surgeons from the department of gastrointestinal surgery at the University Hospital of North Norway. The results confirmed that interruptions from mobile devices were a problem, especially in surgical theatres, outpatient wards, emergency wards and inpatient rooms. Users in hospitals, especially surgeons and physicians, need a better communication system. Our proposed system would intercept the signals from the existing communication system before they are sent out to the mobile devices. The signals would then be routed through a context-aware system, paired with context information and available rules defined by the doctor, which will decide what to do with the call/page. A single device which integrates both the pager and the phone system, and makes use of context information to control interruptions automatically yet allow the caller to decide whether to interrupt, would be highly appreciated by the users.
Video quality of 3G videophones for telephone cardiopulmonary resuscitation
Authors: Tränkler Uwe , Hagen Oddvar , Horsch Alexander
Published: October 2008

We simulated a cardiopulmonary resuscitation (CPR) scene with a manikin and used two 3G videophones on the caller's side to transmit video to a laptop PC. Five observers (two doctors with experience in emergency medicine and three paramedics) evaluated the video. They judged whether the manikin was breathing and whether they would give advice for CPR; they also graded the confidence of their decision-making. Breathing was only visible from certain orientations of the videophones, at distances below 150 cm with good illumination and a still background. Since the phones produced a degradation in colours and shadows, detection of breathing mainly depended on moving contours. Low camera positioning produced better results than having the camera high up. Darkness, shaking of the camera and a moving background made detection of breathing almost impossible. The video from the two 3G videophones that were tested was of sufficient quality for telephone CPR provided that camera orientation, distance, illumination and background were carefully chosen. Thus it seems possible to use 3G videophones for emergency calls involving CPR. However, further studies on the required video quality in different scenarios are necessary.