bluuebunny/crossref_metadata_2025 huggingface dataset:
Spontaneous and Stimulated Undulator Radiation in Symmetric and Asymmetric Multi-Periodic Magnetic Fields
Authors: Zhukovsky Konstantin , Fedorov Igor
Published: January 2021

In this work, the radiation from electrons in multi-periodic undulator fields with symmetric and asymmetric harmonics was analyzed using generalized Bessel functions formalism. The asymmetric, symmetric, and anti-symmetric periodic magnetic fields with harmonics were studied in order to get the enhanced radiation of the high harmonics of undulator radiation (UR). The effect on the spontaneous and stimulated UR was explored. The exact integral forms for the Bessel coefficients were obtained for undulators with general symmetric and asymmetric field harmonics. Spectral properties of the radiation from several configurations of the undulator fields with harmonics were compared with each other. The resulting spontaneous UR spectrum and harmonic intensities were obtained analytically in the form of integrals and compared with the respective results that were obtained numerically with SPECTRA program. The dimensionless scaling parameter of a free electron laser (FEL)—the Pierce parameter (ρ)—was computed and compared for the different considered undulators. We studied the differences in the behavior of the high-gain single pass FEL harmonics and the spontaneous UR harmonics in the same undulators. The undulators with variable deflection parameter (k) were considered. The effect of the k parameter (deflection parameter for a common planar undulator) on the spontaneous UR and on the high-gain FEL radiation was explored. In this context, an experiment with variable strength undulators at FLASH 2 FEL was analyzed; the shorter saturated length in high harmonic self-seeding (HHSS) regime vs. self-amplified spontaneous emission (SASE) is explained.
Behavior of the Eigenvalues and Eignfunctions of the Regge-Type Problem
Authors: Jwamer Karwan H. F. , Rasul Rando R. Q.
Published: January 2021

This article investigates the spectral theory of the problem of the Regge-type with transmission conditions and discontinuous coefficients. We formulate a new linear operator, by which we can deal with simplicity and boundedness of the eigenpairs of the problem. The aim of this work is to conduct that the problem has an infinite number of simple positive eigenvalues.
Deep Learning for Spectrum Sensing in Cognitive Radio
Authors: Solanki Surendra , Dehalwar Vasudev , Choudhary Jaytrilok
Published: January 2021

The detection of primary user signals is essential for optimum utilization of a spectrum by secondary users in cognitive radio (CR). The conventional spectrum sensing schemes have the problem of missed detection/false alarm, which hampers the proper utilization of spectrum. Spectrum sensing through deep learning minimizes the margin of error in the detection of the free spectrum. This research provides an insight into using a deep neural network for spectrum sensing. A deep learning based model, “DLSenseNet”, is proposed, which exploits structural information of received modulated signals for spectrum sensing. The experiments were performed using RadioML2016.10b dataset and the outcome was studied. It was found that “DLSenseNet” provides better spectrum detection than other sensing models.
The Symmetric Nature of the Position Distribution of the Human Body Center of Gravity during Propelling Manual Wheelchairs with Innovative Propulsion Systems
Authors: Wieczorek Bartosz , Kukla Mateusz , Warguła Łukasz
Published: January 2021

Objective: The main objective of the tests conducted was to analyze the position variability of the human body’s center of gravity during propelling the wheelchair, and to demonstrate the properties enabling the description of this variability by means of plane figures with a symmetry axis. A secondary objective was to show the impact of the used manual propulsion type and the wheelchair inclination angle in relation to the plane on the dimensions of the position variability areas of the center of gravity. Method and materials: Three patients participated in the research representing 50 centiles of anthropometric dimensions. Each patient carried out fifteen measurement tests on three wheelchairs for three inclination angles of the wheelchair frame in relation to the level. Each measurement test consisted of five propulsion cycles for which the positions of the center of gravity were determined with the sampling frequency of 100 Hz. The measured positions of the center of gravity were approximated with ellipses containing 95.4% of the measurements conducted, assuming their dimension scaling basis in the form of the double value of standard deviation defined based on the registered results. Results: Based on the measurements conducted, the average values of five ellipses parameters were determined for nine cases in which a variable was the type of wheelchair propulsion and its inclination angle in relation to the level. The area of the highest variability of the position of the center of gravity was measured for the wheelchair with a multispeed transmission. The average dimensions of the ellipse semi-axis amounted to 108.53 mm for the semi-axis a and 29.75 for the semi-axis b, the average position of the ellipse center amounted to x = 114.51 mm and y = −10.53 mm, and the average inclination angle of the ellipse α amounted to −6.92°. The area of the lowest variability of the position of the center of gravity was measured for the wheelchair with a hybrid transmission. In this case, the average dimensions of the ellipse semi-axis amounted to 64.07 mm for the semi-axis a and 33.85 for the semi-axis b; whereas, the average position of the ellipse center amounted to x = 245.13 mm and y = −28.24 mm, and the average inclination angle of the ellipse α amounted to −0.56°.
Small-Signal Stability of Multi-Converter Infeed Power Grids with Symmetry
Authors: Yu Jiawei , Yang Ziqian , Kurths Jurgen , Zhan Meng
Published: January 2021

Traditional power systems have been gradually shifting to power-electronic-based ones, with more power electronic devices (including converters) incorporated recently. Faced with much more complicated dynamics, it is a great challenge to uncover its physical mechanisms for system stability and/or instability (oscillation). In this paper, we first establish a nonlinear model of a multi-converter power system within the DC-link voltage timescale, from the first principle. Then, we obtain a linearized model with the associated characteristic matrix, whose eigenvalues determine the system stability, and finally get independent subsystems by using symmetry approximation conditions under the assumptions that all converters’ parameters and their susceptance to the infinite bus (Bg) are identical. Based on these mathematical analyses, we find that the whole system can be decomposed into several equivalent single-converter systems and its small-signal stability is solely determined by a simple converter system connected to an infinite bus under the same susceptance Bg. These results of large-scale multi-converter analysis help to understand the power-electronic-based power system dynamics, such as renewable energy integration. As well, they are expected to stimulate broad interests among researchers in the fields of network dynamics theory and applications.
Existence of the Solutions of Nonlinear Fractional Differential Equations Using the Fixed Point Technique in Extended b-Metric Spaces
Authors: Guran Liliana , Bota Monica-Felicia
Published: January 2021

The purpose of this paper is to prove fixed point theorems for cyclic-type operators in extended b-metric spaces. The well-posedness of the fixed point problem and limit shadowing property are also discussed. Some examples are given in order to support our results, and the last part of the paper considers some applications of the main results. The first part of this section is devoted to the study of the existence of a solution to the boundary value problem. In the second part of this section, we study the existence of solutions to fractional boundary value problems with integral-type boundary conditions in the frame of some Caputo-type fractional operators.
Nucleon Structure and Spin Effects in Elastic Hadron Scattering
Authors: Selyugin Oleg
Published: January 2021

Soft diffraction phenomena in elastic nucleon scattering are considered from the viewpoint of the spin dependence of the interaction potential. Spin-dependent pomeron effects are analyzed for elastic pp scattering, and spin-dependent differential cross sections and spin correlation parameters are calculated. The spin correlation parameter AN is examined on the basis of experimental data from s=4.9 GeV up to 23.4GeV in the framework of the extended High Energy Generalized Structure (HEGS) model. It is shown that the existing experimental data of proton-proton and proton-antiproton elastic scattering at high energy in the region of the diffraction minimum and at large momentum transfer give the support of the existence of the energy-independent part of the hadron spin flip amplitude.
Acknowledgment to Reviewers of Telecom in 2020
Authors:   Telecom Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Telecom maintains its standards for the high quality of its published papers [...]
Switching Shiga Toxin (Stx) Type from Stx2d to Stx2a but Not Stx2c Alters Virulence of Stx-Producing Escherichia coli (STEC) Strain B2F1 in Streptomycin (Str)-Treated Mice
Authors: McNichol Beth A. , Bova Rebecca A. , Torres Kieron , Preston Lan N. , Melton-Celsa Angela R.
Published: January 2021

Shiga toxin (Stx)-producing Escherichia coli (STEC) strain B2F1 produces Stx type 2d, a toxin that becomes more toxic towards Vero cells in the presence of intestinal mucus. STEC that make Stx2d are more pathogenic to streptomycin (Str)-treated mice than most STEC that produce Stx2a or Stx2c. However, purified Stx2d is only 2- or 7-fold more toxic by the intraperitoneal route than Stx2a or Stx2c, respectively. We hypothesized, therefore, that the toxicity differences among Stx2a, Stx2c, and Stx2d occur at the level of delivery from the intestine. To evaluate that hypothesis, we altered the toxin type produced by stx2d+ mouse virulent O91:H21 clinical isolate B2F1 to Stx2a or Stx2c. Because B2F1 encodes two copies of stx2d, we did these studies in a derivative of B2F1 in which stx2d1 was deleted. Although the strains were equivalently virulent to the Str-treated mice at the 1010 dose, the B2F1 strain that produced Stx2a was attenuated relative to the ones that produced Stx2d or Stx2c when administered at 103 CFU/mouse. We next compared the oral toxicities of purified Stx2a, Stx2c, and Stx2d. We found that purified Stx2d is more toxic than Stx2a or Stx2c upon oral administration at 4 µg/mouse. Taken together, these studies suggest that Stx2 toxins are most potent when delivered directly from the bacterium. Furthermore, because Stx2d and Stx2c have the identical amino acid composition in the toxin B subunit, our results indicate that the virulence difference between Stx2a and Stx2d and Stx2c resides in the B or binding subunit of the toxins.
Toxic Effect of Aflatoxins in Dogs Fed Contaminated Commercial Dry Feed: A Review
Authors: Martínez-Martínez Lizbeth , Valdivia-Flores Arturo G. , Guerrero-Barrera Alma Lilian , Quezada-Tristán Teódulo , Rangel-Muñoz Erika Janet , Ortiz-Martínez Raúl
Published: January 2021

Since its first patent (1897), commercial dry feed (CDF) for dogs has diversified its formulation to meet the nutritional needs of different breeds, age, or special conditions and establish a foundation for integration of these pets into urban lifestyles. The risk of aflatoxicosis in dogs has increased because the ingredients used to formulate CDF have also proliferated, making it difficult to ensure the quality required of each to achieve the safety of the entire CDF. This review contains a description of the fungi and aflatoxins detected in CDF and the ingredients commonly used for their formulation. The mechanisms of action and pathogenic effects of aflatoxins are outlined; as well as the clinical findings, and macroscopic and microscopic lesions found in aflatoxicosis in dogs. In addition, alternatives for diagnosis, treatment, and control of aflatoxins (AF) in CDF are analyzed, such as biomarkers of effect, improvement of blood coagulation, rate of elimination of AF, control of secondary infection, protection of gastric mucosa, reduction of oxidative stress, use of chemo-protectors, sequestrants, grain-free CDF, biocontrol, and maximum permitted limits, are also included.
A Wolf in Another Wolf’s Clothing: Post-Genomic Regulation Dictates Venom Profiles of Medically-Important Cryptic Kraits in India
Authors: Sunagar Kartik , Khochare Suyog , Senji Laxme R. R. , Attarde Saurabh , Dam Paulomi , Suranse Vivek , Khaire Anil , Martin Gerard , Captain Ashok
Published: January 2021

The Common Krait (Bungarus caeruleus) shares a distribution range with many other ‘phenotypically-similar’ kraits across the Indian subcontinent. Despite several reports of fatal envenomings by other Bungarus species, commercial Indian antivenoms are only manufactured against B. caeruleus. It is, therefore, imperative to understand the distribution of genetically distinct lineages of kraits, the compositional differences in their venoms, and the consequent impact of venom variation on the (pre)clinical effectiveness of antivenom therapy. To address this knowledge gap, we conducted phylogenetic and comparative venomics investigations of kraits in Southern and Western India. Phylogenetic reconstructions using mitochondrial markers revealed a new species of krait, Romulus’ krait (Bungarus romulusi sp. nov.), in Southern India. Additionally, we found that kraits with 17 mid-body dorsal scale rows in Western India do not represent a subspecies of the Sind Krait (B. sindanus walli) as previously believed, but are genetically very similar to B. sindanus in Pakistan. Furthermore, venom proteomics and comparative transcriptomics revealed completely contrasting venom profiles. While the venom gland transcriptomes of all three species were highly similar, venom proteomes and toxicity profiles differed significantly, suggesting the prominent role of post-genomic regulatory mechanisms in shaping the venoms of these cryptic kraits. In vitro venom recognition and in vivo neutralisation experiments revealed a strong negative impact of venom variability on the preclinical performance of commercial antivenoms. While the venom of B. caeruleus was neutralised as per the manufacturer’s claim, performance against the venoms of B. sindanus and B. romulusi was poor, highlighting the need for regionally-effective antivenoms in India.
Molecular Characterization of the Enterohemolysin Gene (ehxA) in Clinical Shiga Toxin-Producing Escherichia coli Isolates
Authors: Hua Ying , Zhang Ji , Jernberg Cecilia , Chromek Milan , Hansson Sverker , Frykman Anne , Xiong Yanwen , Wan Chengsong , Matussek Andreas , Bai Xiangning
Published: January 2021

Shiga toxin (Stx)-producing Escherichia coli (STEC) is an important foodborne pathogen with the ability to cause bloody diarrhea (BD) and hemolytic uremic syndrome (HUS). Little is known about enterohemolysin-encoded by ehxA. Here we investigated the prevalence and diversity of ehxA in 239 STEC isolates from human clinical samples. In total, 199 out of 239 isolates (83.26%) were ehxA positive, and ehxA was significantly overrepresented in isolates carrying stx2a + stx2c (p < 0.001) and eae (p < 0.001). The presence of ehxA was significantly associated with BD and serotype O157:H7. Five ehxA subtypes were identified, among which, ehxA subtypes B, C, and F were overrepresented in eae-positive isolates. All O157:H7 isolates carried ehxA subtype B, which was related to BD and HUS. Three ehxA groups were observed in the phylogenetic analysis, namely, group Ⅰ (ehxA subtype A), group Ⅱ (ehxA subtype B, C, and F), and group Ⅲ (ehxA subtype D). Most BD- and HUS-associated isolates were clustered into ehxA group Ⅱ, while ehxA group Ⅰ was associated with non-bloody stool and individuals ≥10 years of age. The presence of ehxA + eae and ehxA + eae + stx2 was significantly associated with HUS and O157:H7 isolates. In summary, this study showed a high prevalence and the considerable genetic diversity of ehxA among clinical STEC isolates. The ehxA genotypes (subtype B and phylogenetic group Ⅱ) could be used as risk predictors, as they were associated with severe clinical symptoms, such as BD and HUS. Furthermore, ehxA, together with stx and eae, can be used as a risk predictor for HUS in STEC infections.
Toxin–Antitoxin Systems in Pathogenic Bacteria
Authors: Alonso Juan C.
Published: January 2021

Toxin–antitoxin (TA) systems, which are ubiquitously present in plasmids, bacterial and archaeal genomes, are classified as types I to VI, according to the nature of the antitoxin and to the mode of toxin inhibition [...]
Genetic Manipulation of the Ergot Alkaloid Pathway in Epichloë festucae var. lolii and Its Effect on Black Beetle Feeding Deterrence
Authors: Hudson Debbie , Mace Wade , Popay Alison , Jensen Joanne , McKenzie Catherine , Cameron Catherine , Johnson Richard
Published: January 2021

Epichloë endophytes are filamentous fungi (family Clavicipitaceae) that live in symbiotic associations with grasses in the sub family Poöideae. In New Zealand, E. festucae var. lolii confers significant resistance to perennial ryegrass (Lolium perenne) against insect and animal herbivory and is an essential component of pastoral agriculture, where ryegrass is a major forage species. The fungus produces in planta a range of bioactive secondary metabolites, including ergovaline, which has demonstrated bioactivity against the important pasture pest black beetle, but can also cause mammalian toxicosis. We genetically modified E. festucae var. lolii strain AR5 to eliminate key enzymatic steps in the ergovaline pathway to determine if intermediate ergot alkaloid compounds can still provide insecticidal benefits in the absence of the toxic end product ergovaline. Four genes (dmaW, easG, cloA, and lpsB) spanning the pathway were deleted and each deletion mutant was inoculated into five different plant genotypes of perennial ryegrass, which were later harvested for a full chemical analysis of the ergot alkaloid compounds produced. These associations were also used in a black beetle feeding deterrence study. Deterrence was seen with just chanoclavine present, but was cumulative as more intermediate compounds in the pathway were made available. Ergovaline was not detected in any of the deletion associations, indicating that bioactivity towards black beetle can be obtained in the absence of this mammalian toxin.
Travel-Related Antimicrobial Resistance: A Systematic Review
Authors: Bokhary Hamid , Pangesti Krisna N. A. , Rashid Harunor , Abd El Ghany Moataz , Hill-Cawthorne Grant A.
Published: January 2021

There is increasing evidence that human movement facilitates the global spread of resistant bacteria and antimicrobial resistance (AMR) genes. We systematically reviewed the literature on the impact of travel on the dissemination of AMR. We searched the databases Medline, EMBASE and SCOPUS from database inception until the end of June 2019. Of the 3052 titles identified, 2253 articles passed the initial screening, of which 238 met the inclusion criteria. The studies covered 30,060 drug-resistant isolates from 26 identified bacterial species. Most were enteric, accounting for 65% of the identified species and 92% of all documented isolates. High-income countries were more likely to be recipient nations for AMR originating from middle- and low-income countries. The most common origin of travellers with resistant bacteria was Asia, covering 36% of the total isolates. Beta-lactams and quinolones were the most documented drug-resistant organisms, accounting for 35% and 31% of the overall drug resistance, respectively. Medical tourism was twice as likely to be associated with multidrug-resistant organisms than general travel. International travel is a vehicle for the transmission of antimicrobial resistance globally. Health systems should identify recent travellers to ensure that adequate precautions are taken.
An Analysis of Self-Build and Custom Housebuilding in the South West of England
Authors: Gingell Annie Hamilton , Shahab Sina
Published: January 2021

This paper seeks to evaluate how successful national policy interventions have been at addressing land barriers to self-build and custom housebuilding when applied by Local Planning Authorities (LPAs) across the South West of England. A longitudinal triangulated mixed method approach was undertaken to comprehensively interrogate the research objective. This comprised submitting a Freedom of Information (FOI) request to each LPA within the study area; an assessment of the most recently produced Strategic Housing Market Assessments (SHMAs); deriving alternative demand estimates using national data as a proxy; and alternate estimates of supply calculated using BuildStore and The Land Bank Partnership plot search websites. The findings of the study revealed that LPA Registers can only be viewed as a minimum assessment of demand for self-build and custom housebuilding and the effectiveness of LPAs in classifying suitable development permissions for self-build and custom housebuilding was highly dependent on the mechanisms used to identify permissions.
Intranasal Infection of Ferrets with SARS-CoV-2 as a Model for Asymptomatic Human Infection
Authors: Everett Helen E. , Lean Fabian Z. X. , Byrne Alexander M. P. , van Diemen Pauline M. , Rhodes Shelley , James Joe , Mollett Benjamin , Coward Vivien J. , Skinner Paul , Warren Caroline J. , Bewley Kevin R. , Watson Samantha , Hurley Shellene , Ryan Kathryn A. , Hall Yper , Simmons Hugh , Núñez Alejandro , Carroll Miles W. , Brown Ian H. , Brookes Sharon M.
Published: January 2021

Ferrets were experimentally inoculated with SARS-CoV-2 (severe acute respiratory syndrome (SARS)-related coronavirus 2) to assess infection dynamics and host response. During the resulting subclinical infection, viral RNA was monitored between 2 and 21 days post-inoculation (dpi), and reached a peak in the upper respiratory cavity between 4 and 6 dpi. Viral genomic sequence analysis in samples from three animals identified the Y453F nucleotide substitution relative to the inoculum. Viral RNA was also detected in environmental samples, specifically in swabs of ferret fur. Microscopy analysis revealed viral protein and RNA in upper respiratory tract tissues, notably in cells of the respiratory and olfactory mucosae of the nasal turbinates, including olfactory neuronal cells. Antibody responses to the spike and nucleoprotein were detected from 21 dpi, but virus-neutralizing activity was low. A second intranasal inoculation (re-exposure) of two ferrets after a 17-day interval did not produce re-initiation of viral RNA shedding, but did amplify the humoral response in one animal. Therefore, ferrets can be experimentally infected with SARS-CoV-2 to model human asymptomatic infection.
Cellular Receptors Involved in KSHV Infection
Authors: van der Meulen Emma , Anderton Meg , Blumenthal Melissa J. , Schäfer Georgia
Published: January 2021

The process of Kaposi’s Sarcoma Herpes Virus’ (KSHV) entry into target cells is complex and engages several viral glycoproteins which bind to a large range of host cell surface molecules. Receptors for KSHV include heparan sulphate proteoglycans (HSPGs), several integrins and Eph receptors, cystine/glutamate antiporter (xCT) and Dendritic Cell-Specific Intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN). This diverse range of potential binding and entry sites allows KSHV to have a broad cell tropism, and entry into specific cells is dependent on the available receptor repertoire. Several molecules involved in KSHV entry have been well characterized, particularly those postulated to be associated with KSHV-associated pathologies such as Kaposi’s Sarcoma (KS). In this review, KSHV infection of specific cell types pertinent to its pathogenesis will be comprehensively summarized with a focus on the specific cell surface binding and entry receptors KSHV exploits to gain access to a variety of cell types. Gaps in the current literature regarding understanding interactions between KSHV glycoproteins and cellular receptors in virus infection are identified which will lead to the development of virus infection intervention strategies.
Quinacrine, an Antimalarial Drug with Strong Activity Inhibiting SARS-CoV-2 Viral Replication In Vitro
Authors: Salas Rojas Mónica , Silva Garcia Raúl , Bini Estela , Pérez de la Cruz Verónica , León Contreras Juan Carlos , Hernández Pando Rogelio , Bastida Gonzalez Fernando , Davila-Gonzalez Eduardo , Orozco Morales Mario , Gamboa Domínguez Armando , Sotelo Julio , Pineda Benjamín
Published: January 2021

Quinacrine (Qx), a molecule used as an antimalarial, has shown anticancer, antiprion, and antiviral activity. The most relevant antiviral activities of Qx are related to its ability to raise pH in acidic organelles, diminishing viral enzymatic activity for viral cell entry, and its ability to bind to viral DNA and RNA. Moreover, Qx has been used as an immunomodulator in cutaneous lupus erythematosus and various rheumatological diseases, by inhibiting phospholipase A2 modulating the Th1/Th2 response. The aim of this study was to evaluate the potential antiviral effect of Qx against denominated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in Vero E6 cells. The cytotoxicity of Qx in Vero E6 cells was determined by the MTT assay. Afterwards, Vero E6 cells were infected with SARS-CoV-2 at different multiplicities of infections (MOIs) of 0.1 and 0.01 in the presence of Qx (0–30 µM) to determinate the half maximal effective concentration (EC50). After 48 h, the effect of Qx against SARS-CoV-2 was assessed by viral cytotoxicity and viral copy numbers, the last were determined by digital real-time RT-PCR (ddRT-PCR). Additionally, electron and confocal microscopy of Vero E6 cells infected and treated with Qx was studied. Our data show that Qx reduces SARS-CoV-2 virus replication and virus cytotoxicity, apparently by inhibition of viral ensemble, as observed by ultrastructural images, suggesting that Qx could be a potential drug for further clinical studies against coronavirus disease 2019 (COVID-19) infection.
Interactions of Viral Proteins from Pathogenic and Low or Non-Pathogenic Orthohantaviruses with Human Type I Interferon Signaling
Authors: Gallo Giulia , Caignard Grégory , Badonnel Karine , Chevreux Guillaume , Terrier Samuel , Szemiel Agnieszka , Roman-Sosa Gleyder , Binder Florian , Gu Quan , Da Silva Filipe Ana , Ulrich Rainer G. , Kohl Alain , Vitour Damien , Tordo Noël , Ermonval Myriam
Published: January 2021

Rodent-borne orthohantaviruses are asymptomatic in their natural reservoir, but they can cause severe diseases in humans. Although an exacerbated immune response relates to hantaviral pathologies, orthohantaviruses have to antagonize the antiviral interferon (IFN) response to successfully propagate in infected cells. We studied interactions of structural and nonstructural (NSs) proteins of pathogenic Puumala (PUUV), low-pathogenic Tula (TULV), and non-pathogenic Prospect Hill (PHV) viruses, with human type I and III IFN (IFN-I and IFN-III) pathways. The NSs proteins of all three viruses inhibited the RIG-I-activated IFNβ promoter, while only the glycoprotein precursor (GPC) of PUUV, or its cleavage product Gn/Gc, and the nucleocapsid (N) of TULV inhibited it. Moreover, the GPC of both PUUV and TULV antagonized the promoter of IFN-stimulated responsive elements (ISRE). Different viral proteins could thus contribute to inhibition of IFNβ response in a viral context. While PUUV and TULV strains replicated similarly, whether expressing entire or truncated NSs proteins, only PUUV encoding a wild type NSs protein led to late IFN expression and activation of IFN-stimulated genes (ISG). This, together with the identification of particular domains of NSs proteins and different biological processes that are associated with cellular proteins in complex with NSs proteins, suggested that the activation of IFN-I is probably not the only antiviral pathway to be counteracted by orthohantaviruses and that NSs proteins could have multiple inhibitory functions.
Effectiveness of the Quadrivalent HPV Vaccine in Preventing Anal ≥ HSILs in a Spanish Population of HIV+ MSM Aged > 26 Years
Authors: Hidalgo-Tenorio Carmen , Pasquau Juan , Omar-Mohamed Mohamed , Sampedro Antonio , López-Ruz Miguel A. , López Hidalgo Javier , Ramírez-Taboada Jessica
Published: January 2021

Anal squamous cell carcinoma is the most frequent virus-related non-AIDS-defining neoplasia among HIV-infected individuals, especially MSM. The objectives of this study were to analyze the effectiveness of the quadrivalent HPV (qHPV) vaccine to prevent anal ≥ high-grade squamous intraepithelial lesions (≥HSILs), external ano-genital lesions (EAGLs), and infection by qHPV vaccine genotypes in HIV+ MSM, and to study the immunogenicity of the vaccine and risk factors for ≥ HSILs. This study is nested within a randomized, double-blind, placebo-controlled trial of the qHPV vaccine, which enrolled participants between May 2012 and May 2014, with a 48-month follow-up. A vaccine or placebo was administered at 0, 2, and 6 months, and vaccine antibody titers were evaluated at 7, 12, 24, 36, and 48 months. Data were gathered at 12, 24, 36, and 48 months on sexual habits, CD4/CD8 cell/counts, HIV viral load, and the results of cytology (Thin Prep® Pap Test), HPV PCR genotyping (Linear Array HPV Genotyping Test), and high-resolution anoscopy (Zeiss 150 fc© colposcope). The study included 129 patients (mean age of 38.8 years, 40 [31%] with a history of AIDS, 119 [92.2%] receiving ART, and 4 [3.3%] with virological failure), 66 (51.2%) in vaccine arm and 63 (48.4%) in placebo arm. The vaccine and placebo groups did not differ in ≥ HSILs (14.1 vs. 13.1%, respectively, p = 0.98) or EAGL (11.1 vs. 6.8%, p = 0.4) rates during follow-up; however, a protective effect against HPV 6 was observed during the first year of follow-up in the vaccine versus placebo group (7.5% vs. 23.4%; p = 0.047). A between-arm difference (p = 0.0001) in antibodies against qHPV vaccine genotypes was observed at 7 months (76.9% in vaccine arm vs. 30.2% in placebo arm), 12 months (68.1% vs. 26.5%), 24 months (75% vs. 32.5%), 36 months (90% vs. 24.4%), and 48 months (87.2% vs. 30%). Finally, the factor associated with the risk of anal ≥ HSIL onset during the four-year follow-up was the receipt of the last dose of the vaccine less than 6 months earlier in comparison to those vaccinated for a longer period (82.4% vs. 17.6% (OR 0.869 [95% CI, 0.825–0.917]). Vaccine and placebo arms did not significantly differ in ≥ HSIL or EAGL rates or in protection against infection by HPV genotype vaccine except for HPV6 at 12 months after the first dose. A long-lasting immune response was observed in almost all the vaccinated men. The main protective factor against ≥ HSIL was to have completed the vaccination regimen more than 6 months earlier.
Biological and Clinical Consequences of Integrin Binding via a Rogue RGD Motif in the SARS CoV-2 Spike Protein
Authors: Makowski Lee , Olson-Sidford William , W. Weisel John
Published: January 2021

Although ACE2 (angiotensin converting enzyme 2) is considered the primary receptor for CoV-2 cell entry, recent reports suggest that alternative pathways may contribute. This paper considers the hypothesis that viral binding to cell-surface integrins may contribute to the high infectivity and widespread extra-pulmonary impacts of the SARS-CoV-2 virus. This potential is suggested on the basis of the emergence of an RGD (arginine-glycine-aspartate) sequence in the receptor-binding domain of the spike protein. RGD is a motif commonly used by viruses to bind cell-surface integrins. Numerous signaling pathways are mediated by integrins and virion binding could lead to dysregulation of these pathways, with consequent tissue damage. Integrins on the surfaces of pneumocytes, endothelial cells and platelets may be vulnerable to CoV-2 virion binding. For instance, binding of intact virions to integrins on alveolar cells could enhance viral entry. Binding of virions to integrins on endothelial cells could activate angiogenic cell signaling pathways; dysregulate integrin-mediated signaling pathways controlling developmental processes; and precipitate endothelial activation to initiate blood clotting. Such a procoagulant state, perhaps together with enhancement of platelet aggregation through virions binding to integrins on platelets, could amplify the production of microthrombi that pose the threat of pulmonary thrombosis and embolism, strokes and other thrombotic consequences. The susceptibility of different tissues to virion–integrin interactions may be modulated by a host of factors, including the conformation of relevant integrins and the impact of the tissue microenvironment on spike protein conformation. Patient-specific differences in these factors may contribute to the high variability of clinical presentation. There is danger that the emergence of receptor-binding domain mutations that increase infectivity may also enhance access of the RGD motif for integrin binding, resulting in viral strains with ACE2 independent routes of cell entry and novel integrin-mediated biological and clinical impacts. The highly infectious variant, B.1.1.7 (or VUI 202012/01), includes a receptor-binding domain amino acid replacement, N501Y, that could potentially provide the RGD motif with enhanced access to cell-surface integrins, with consequent clinical impacts.
The Capsid Protein of Rubella Virus Antagonizes RNA Interference in Mammalian Cells
Authors: Xu Jiuyue , Kong Jing , Lyu Bao , Wang Xiaotong , Qian Qi , Zhou Xi , Qiu Yang
Published: January 2021

Rubella virus (RuV) is the infectious agent of a series of birth defect diseases termed congenital rubella syndrome, which is a major public health concern all around the world. RNA interference (RNAi) is a crucial antiviral defense mechanism in eukaryotes, and numerous viruses have been found to encode viral suppressors of RNAi (VSRs) to evade antiviral RNAi response. However, there is little knowledge about whether and how RuV antagonizes RNAi. In this study, we identified that the RuV capsid protein is a potent VSR that can efficiently suppress shRNA- and siRNA-induced RNAi in mammalian cells. Moreover, the VSR activity of the RuV capsid is dependent on its dimerization and double-stranded RNA (dsRNA)-binding activity. In addition, ectopic expression of the RuV capsid can effectively rescue the replication defect of a VSR-deficient virus or replicon, implying that the RuV capsid can act as a VSR in the context of viral infection. Together, our findings uncover that RuV encodes a VSR to evade antiviral RNAi response, which expands our understanding of RuV–host interaction and sheds light on the potential therapeutic target against RuV.
Digital Development Process for the Drive System of a Balanced Two-Wheel Scooter
Authors: Holder Kevin , Schumacher Sven , Friedrich Matthias , Till Markus , Stetter Ralf , Fichter Walter , Rudolph Stephan
Published: January 2021

Graph-based design languages have received increasing attention in the research community, because they offer a promising approach to address several major issues in engineering, e.g., the frequent manual data transfer between computer-aided design (CAD) and computer-aided engineering (CAE) systems. Currently, these issues prevent the realization of machine executable digital design processes of complex systems such as vehicles. Promising scenarios for urban transportation include an interconnection of mass transportation systems such as buses and subways with individual vehicles for the so-called “last mile” transport. For several reasons, these vehicles should be as small and light as possible. A considerable reduction in weight and size can be achieved, if such vehicles are tailored to the individual size, weight and proportion of the individual user. However, tailoring vehicles for the individual characteristics of each user go beyond a simple building set and require a continuous digital design process. Consequently, the topic of this paper is a digital design process of a self-balanced scooter, which can be used as an individual last-mile means of transport. This process is based on graph-based design languages, because in these languages, a digital system model is generated, which contains all relevant information about a design and can be fed into any simulation tool which is needed to evaluate the impact of a possible design variation on the resulting product performance. As this process can be automated by digital compilers, it is possible to perform systematic design variations for an almost infinite amount of parameters and topological variants. Consequently, these kinds of graph-based languages are a powerful means to generate viable design alternatives and thus permit fast evaluations. The paper demonstrates the design process, focusing on the drive system of the respective balanced two-wheel scooter and highlights the advantages (data integration and possibility for machine execution).
The Complex Diseases of Staphylococcus pseudintermedius in Canines: Where to Next?
Authors: Lynch Stephanie A. , Helbig Karla J.
Published: January 2021

Staphylococcus pseudintermedius is a pathogenic bacterium of concern within the veterinary sector and is involved in numerous infections in canines, including topical infections such as canine pyoderma and otitis externa, as well as systemic infections within the urinary, respiratory and reproductive tract. The high prevalence of methicillin-resistant Staphylococcus pseudintermedius (MRSP) within such infections is a growing concern. Therefore, it is crucial to understand the involvement of S. pseudintermedius in canine disease pathology to gain better insight into novel treatment avenues. Here, we review the literature focused on S. pseudintermedius infection in multiple anatomic locations in dogs and the role of MRSP in treatment outcomes at these niches. Multiple novel treatment avenues for MRSP have been pioneered in recent years and these are discussed with a specific focus on vaccines and phage therapy as potential therapeutic options. Whilst both undertakings are in their infancy, phage therapy is versatile and has shown high success in both animal and human medical use. It is clear that further research is required to combat the growing problems associated with MRSP in canines.
A Comparative Study of Corneal Topography in Children with Autism Spectrum Disorder: A Cross-Sectional Study
Authors: ALGarzaie Maha A. , Alsaqr Ali M.
Published: January 2021

Purpose: To investigate the corneal characteristics in individuals with autism spectrum disorder (ASD) and age-matched typical development (TD) participants. Methods: This cross-sectional, clinically based study compared children with ASD to age-matched TD participants. Corneal topography was measured with a portable EyeSys Vista system. The distance visual acuity (VA) and the contrast sensitivity (CS) were determined. The refractive error (RE) was assessed using a 2WIN autorefractometer. Results: A total of 31 children with ASD (mean age: 12.78 ± 4.49 years), and 60 participants with TD (mean age: 13.65 ± 3.56 years) were recruited. The two groups were similar in age (t = −2.084, p = 0.075) and VA (t = −0.35, p = 0.32). Most of the children with ASD had a significant amount of refractive errors (REs; range: +5.25 to −5.50 DS), and astigmatism was dominant (range: −0.25 to −4.50 DC). There was no statistically significant difference between both groups in terms of average corneal power (t = 1.12, p = 0.39). The children with ASD and participants with TD also did not differ significantly in terms of corneal shape descriptors (p > 0.05), such as corneal asphericity, inferior superior index, opposite sector index, and differential sector index. The spherical equivalent did not differ significantly between the ASD participants and participants with TD (t = 1.15, p = 0.15). There was a significant difference (p < 0.05) in the astigmatism component between the ASD participants and the participants with TD.
Improving Runoff Simulation and Forecasting with Segmenting Delay of Baseflow from Fast Surface Flow in Montane High-Vegetation-Covered Catchments
Authors: Li You , Wang Genxu , Liu Changjun , Lin Shan , Guan Minghong , Zhao Xuantao
Published: January 2021

Due to the complicated terrain conditions in montane catchments, runoff formation is fast and complicated, making accurate simulation and forecasting a significant hydrological challenge. In this study, the spatiotemporal variable source mixed runoff generation module (SVSMRG) was integrated with the long short-term memory (LSTM) method, to develop a semi-distributed model (SVSMRG)-based surface flow and baseflow segmentation (SVSMRG-SBS). Herein, the baseflow was treated as a black box and forecasted using LSTM, while the surface flow was simulated using the SVSMRG module based on hydrological response units (HRUs) constructed using eco-geomorphological units. In the case study, four typical montane catchments with different climatic conditions and high vegetation coverage, located in the topographically varying mountains of the eastern Tibetan Plateau, were selected for runoff and flood process simulations using the proposed SVSMRG-SBS model. The results showed that this model had good performance in hourly runoff and flood process simulations for montane catchments. Regarding runoff simulations, the Nash–Sutcliffe efficiency coefficient (NSE) and correlation coefficient (R2) reached 0.8241 and 0.9097, respectively. Meanwhile, for the flood simulations, the NSE ranged from 0.5923 to 0.7467, and R2 ranged from 0.6669 to 0.8092. For the 1-, 3-, and 5-h baseflow forecasting with the LSTM method, it was found that model performances declined when simulating the runoff processes, wherein the NSE and R2 between the measured and modeled runoff decreased from 0.8216 to 0.8087 and from 0.9095 to 0.8871, respectively. Similar results were found in the flood simulations, the NSE and R2 values declined from 0.7414–0.5885 to 0.7429–0.5716 and from 0.8042–0.6547 to 0.7936–0.6067, respectively. This means that this new model achieved perfect performance in montane catchment runoff and flood simulation and forecasting with 1-, 3-, 5-h steps. Therefore, as it considers vegetation regulation, the SVSMRG-SBS model is expected to improve runoff and flood simulation accuracy in montane high-vegetation-covered catchments.
Monetary Valuation of Flood Protection Ecosystem Service Based on Hydrological Modelling and Avoided Damage Costs. An Example from the Čierny Hron River Basin, Slovakia
Authors: Gallay Igor , Olah Branislav , Gallayová Zuzana , Lepeška Tomáš
Published: January 2021

Flood protection is considered one of the crucial regulating ecosystem services due to climate change and extreme weather events. As an ecosystem service, it combines the results of hydrological and ecosystem research and their implementation into land management and/or planning processes including several formally separated economic sectors. As managerial and economic interests often diverge, successful decision-making requires a common denominator in form of monetary valuation of competing trade-offs. In this paper, a methodical approach based on the monetary value of the ecosystem service provided by the ecosystem corresponding to its actual share in flood regulating processes and the value of the property protected by this service was developed and demonstrated based on an example of a medium size mountain basin (290 ha). Hydrological modelling methods (SWAT, HEC-RAS) were applied for assessing the extent of floods with different rainfalls and land uses. The rainfall threshold value that would cause flooding with the current land use but that would be safely drained if the basin was covered completely by forest was estimated. The cost of the flood protection ecosystem service was assessed by the method of non-market monetary value for estimating avoided damage costs of endangered infrastructure and calculated both for the current and hypothetical land use. The results identify areas that are crucial for water retention and that deserve greater attention in management. In addition, the monetary valuation of flood protection provided by the current but also by hypothetical land uses enables competent and well-formulated decision-making processes.
Toward an Efficient Uncertainty Quantification of Streamflow Predictions Using Sparse Polynomial Chaos Expansion
Authors: Tran Vinh Ngoc , Kim Jongho
Published: January 2021

Reliable hydrologic models are essential for planning, designing, and management of water resources. However, predictions by hydrological models are prone to errors due to a variety of sources of uncertainty. More accurate quantification of these uncertainties using a large number of ensembles and model runs is hampered by the high computational burden. In this study, we developed a highly efficient surrogate model constructed by sparse polynomial chaos expansion (SPCE) coupled with the least angle regression method, which enables efficient uncertainty quantifications. Polynomial chaos expansion was employed to surrogate a storage function-based hydrological model (SFM) for nine streamflow events in the Hongcheon watershed of South Korea. The efficiency of SPCE is investigated by comparing it with another surrogate model, full polynomial chaos expansion (FPCE) built by a well-known, ordinary least square regression (OLS) method. This study confirms that (1) the performance of SPCE is superior to that of FPCE because SPCE can build a more accurate surrogate model (i.e., smaller leave-one-out cross-validation error) with one-quarter the size (i.e., 500 versus 2000). (2) SPCE can sufficiently capture the uncertainty of the streamflow, which is comparable to that of SFM. (3) Sensitivity analysis attained through visual inspection and mathematical computation of the Sobol’ index has been of great success for SPCE to capture the parameter sensitivity of SFM, identifying four parameters, α, Kbas, Pbas, and Pchn, that are most sensitive to the likelihood function, Nash-Sutcliffe efficiency. (4) The computational power of SPCE is about 200 times faster than that of SFM and about four times faster than that of FPCE. The SPCE approach builds a surrogate model quickly and robustly with a more compact experimental design compared to FPCE. Ultimately, it will benefit ensemble streamflow forecasting studies, which must provide information and alerts in real time.
Evaluation of Hydrological Alterations at the Sub-Daily Scale Caused by a Small Hydroelectric Facility
Authors: Braun-Cruz Camila C. , Tritico Hans Mario , Beregula Renato Leandro , Girard Pierre , Zeilhofer Peter , Ribeiro Letícia de Souza , Fantin-Cruz Ibraim
Published: January 2021

This work aims to evaluate the hydrologic changes caused by a small hydropower plant on the watercourse in which it is installed. Since hydrologic research with data of temporal frequencies less than a day is less common than daily measurements, there are few indicators and methodologies capable of treating such records. For this reason, 17 indicators are proposed which describe the magnitude, duration, frequency and rate of changes in hydrologic conditions occurring in a watercourse at a sub-daily frequency. These 17 indicators were used to assess changes in the flow regimes at sub-daily scales across the Itiquira hydroelectric facility in Mato Grosso, Brazil. During the dry season the river was more susceptible to hydroelectric operations than during the wet season. Eighty-eight percent of the proposed indicators were significantly altered during the dry season compared to 71% during the rainy season. In addition to the number of indicators that changed between the seasons, the magnitude of the change was different. During the dry season, 53% of the magnitudes of the proposed indicators were classified as having a high magnitude of change, while in the rainy season only 6% of the indicators were characterized as having a high magnitude of change.