bluuebunny/crossref_metadata_2025 huggingface dataset:
Pattern of Radiotherapy Treatment in Low-Risk, Intermediate-Risk, and High-Risk Prostate Cancer Patients: Analysis of National Cancer Database
Authors: Agrawal Rishabh , Dey Asoke , Datta Sujay , Nassar Ana , Grubb William , Traughber Bryan , Biswas Tithi , Ove Roger , Podder Tarun
Published: November 2022

Background: In this study, the utilization rates and survival outcomes of different radiotherapy techniques are compared in prostate cancer (PCa) patients stratified by risk group. Methods: We analyzed an extensive data set of N0, M0, non-surgical PCa patients diagnosed between 2004 and 2015 from the National Cancer Database (NCDB). Patients were grouped into six categories based on RT modality: an intensity-modulated radiation therapy (IMRT) group with brachytherapy (BT) boost, IMRT with/without IMRT boost, proton therapy, stereotactic body radiation therapy (SBRT), low-dose-rate brachytherapy (BT LDR), and high-dose-rate brachytherapy (BT HDR). Patients were also stratified by the National Comprehensive Cancer Network (NCCN) guidelines: low-risk (clinical stage T1–T2a, Gleason Score (GS) ≤ 6, and Prostate-Specific Antigen (PSA) < 10), intermediate-risk (clinical stage T2b or T2c, GS of 7, or PSA of 10–20), and high-risk (clinical stage T3–T4, or GS of 8–10, or PSA > 20). Overall survival (OS) probability was determined using a Kaplan–Meier estimator. Univariate and multivariate analyses were performed by risk group for the six treatment modalities. Results: The most utilized treatment modality for all PCa patients was IMRT (53.1%). Over the years, a steady increase in SBRT utilization was observed, whereas BT HDR usage declined. IMRT-treated patient groups exhibited relatively lower survival probability in all risk categories. A slightly better survival probability was observed for the proton therapy group. Hormonal therapy was used for a large number of patients in all risk groups. Conclusion: This study revealed that IMRT was the most common treatment modality for PCa patients. Brachytherapy, SBRT, and IMRT+BT exhibited similar survival rates, whereas proton showed slightly better overall survival across the three risk groups. However, analysis of the demographics indicates that these differences are at least in part due to selection bias.
Radioresistance Mechanisms in Prostate Cancer Cell Lines Surviving Ultra-Hypo-Fractionated EBRT: Implications and Possible Clinical Applications
Authors: Sideri Silvia , Petragnano Francesco , Maggio Roberto , Petrungaro Simonetta , Catizone Angela , Gesualdi Luisa , De Martino Viviana , Battafarano Giulia , Del Fattore Andrea , Liguoro Domenico , De Cesaris Paola , Filippini Antonio , Marampon Francesco , Riccioli Anna
Published: November 2022

The use of a higher dose per fraction to overcome the high radioresistance of prostate cancer cells has been unsuccessfully proposed. Herein, we present PC3 and DU-145, castration-resistant prostate cancer cell lines that survived a clinically used ultra-higher dose per fraction, namely, radioresistant PC3 and DU-145 cells (PC3RR and DU-145RR). Compared to PC3, PC3RR showed a higher level of aggressive behaviour, with enhanced clonogenic potential, DNA damage repair, migration ability and cancer stem cell features. Furthermore, compared to PC3, PC3RR more efficiently survived further radiation by increasing proliferation and down-regulating pro-apoptotic proteins. No significant changes of the above parameters were described in DU-145RR, suggesting that different prostate cancer cell lines that survive ultra-higher dose per fraction do not display the same grade of aggressive phenotype. Furthermore, both PC3RR and DU-145RR increased antioxidant enzymes and mesenchymal markers. Our data suggest that different molecular mechanisms could be potential targets for future treatments plans based on sequential strategies and synergistic effects of different modalities, possibly in a patient-tailored fashion. Moreover, PC3RR cells displayed an increase in specific markers involved in bone remodeling, indicating that radiotherapy selects a PC3 population capable of migrating to secondary metastatic sites. Finally, PC3RR cells showed a better sensitivity to Docetaxel as compared to native PC3 cells. This suggests that a subset of patients with castration-resistant metastatic disease could benefit from upfront Docetaxel treatment after the failure of radiotherapy.
Transcriptomic and Proteomic Profiles for Elucidating Cisplatin Resistance in Head-and-Neck Squamous Cell Carcinoma
Authors: Garcia-Mayea Yoelsis , Benítez-Álvarez Lisandra , Sánchez-García Almudena , Bataller Marina , Companioni Osmel , Mir Cristina , Benavente Sergi , Lorente Juan , Canela Nuria , Fernández-Rozadilla Ceres , Carracedo Angel , LLeonart Matilde E.
Published: November 2022

To identify the novel genes involved in chemoresistance in head and neck squamous cell carcinoma (HNSCC), we explored the expression profiles of the following cisplatin (CDDP) resistant (R) versus parental (sensitive) cell lines by RNA-sequencing (RNA-seq): JHU029, HTB-43 and CCL-138. Using the parental condition as a control, 30 upregulated and 85 downregulated genes were identified for JHU029-R cells; 263 upregulated and 392 downregulated genes for HTB-43-R cells, and 154 upregulated and 68 downregulated genes for CCL-138-R cells. Moreover, we crossed-checked the RNA-seq results with the proteomic profiles of HTB-43-R (versus HTB-43) and CCL-138-R (versus CCL-138) cell lines. For the HTB-43-R cells, 21 upregulated and 72 downregulated targets overlapped between the proteomic and transcriptomic data; whereas in CCL-138-R cells, four upregulated and three downregulated targets matched. Following an extensive literature search, six genes from the RNA-seq (CLDN1, MAGEB2, CD24, CEACAM6, IL1B and ISG15) and six genes from the RNA-seq and proteomics crossover (AKR1C3, TNFAIP2, RAB7A, LGALS3BP, PSCA and SSRP1) were selected to be studied by qRT-PCR in 11 HNSCC patients: six resistant and five sensitive to conventional therapy. Interestingly, the high MAGEB2 expression was associated with resistant tumours and is revealed as a novel target to sensitise resistant cells to therapy in HNSCC patients.
Improving Catalytic Activity towards the Direct Synthesis of H2O2 through Cu Incorporation into AuPd Catalysts
Authors: Barnes Alexandra , Lewis Richard J. , Morgan David J. , Davies Thomas E. , Hutchings Graham J.
Published: November 2022

With a focus on catalysts prepared by an excess-chloride wet impregnation procedure and supported on the zeolite ZSM-5(30), the introduction of low concentrations of tertiary base metals, in particular Cu, into supported AuPd nanoparticles can be observed to enhance catalytic activity towards the direct synthesis of H2O2. Indeed the optimal catalyst formulation (1%AuPd(0.975)Cu(0.025)/ZSM-5) is able to achieve rates of H2O2 synthesis (115 molH2O2kgcat−1h−1) approximately 1.7 times that of the bi-metallic analogue (69 molH2O2kgcat−1h−1) and rival that previously reported over comparable materials which use Pt as a dopant. Notably, the introduction of Cu at higher loadings results in an inhibition of performance. Detailed analysis by CO-DRFITS and XPS reveals that the improved performance observed over the optimal catalyst can be attributed to the electronic modification of the Pd species and the formation of domains of a mixed Pd2+/Pd0 oxidation state as well as structural changed within the nanoalloy.
Review on the Energy Transformation Application of Black Phosphorus and Its Composites
Authors: Liu Hao , Peng Zehui , Hu Mengdi , Xu Xin , Lou Shuai , Yan Shancheng
Published: November 2022

Black phosphorus (BP) is a unique two-dimensional material with excellent conductivity, and a widely tunable bandgap. In recent years, its application in the field of energy has attracted extensive attention, in terms of energy storage, due to its high theoretical specific capacity and excellent conductivity, black phosphorus is widely used as electrode material in battery and supercapacitors, while for energy generating, it has been also used as photocatalyst and electrocatalysts to split water and produce hydrogen. Black phosphorus demonstrates even better stability and catalytic performance through further construction, doping, or heterojunction. This review briefly summarizes the latest research progress of black phosphorus and its composites in energy preparation and storage, as well as ammonia nitrogen fixation, and also looks into the possible development directions in the future.
Changes in Mitochondrial Size and Morphology in the RPE and Photoreceptors of the Developing and Ageing Zebrafish
Authors: Burgoyne Thomas , Toms Maria , Way Chris , Tracey-White Dhani , Futter Clare E. , Moosajee Mariya
Published: November 2022

Mitochondria are essential adenosine triphosphate (ATP)-generating cellular organelles. In the retina, they are highly numerous in the photoreceptors and retinal pigment epithelium (RPE) due to their high energetic requirements. Fission and fusion of the mitochondria within these cells allow them to adapt to changing demands over the lifespan of the organism. Using transmission electron microscopy, we examined the mitochondrial ultrastructure of zebrafish photoreceptors and RPE from 5 days post fertilisation (dpf) through to late adulthood (3 years). Notably, mitochondria in the youngest animals were large and irregular shaped with a loose cristae architecture, but by 8 dpf they had reduced in size and expanded in number with more defined cristae. Investigation of temporal gene expression of several mitochondrial-related markers indicated fission as the dominant mechanism contributing to the changes observed over time. This is likely to be due to continued mitochondrial stress resulting from the oxidative environment of the retina and prolonged light exposure. We have characterised retinal mitochondrial ageing in a key vertebrate model organism, that provides a basis for future studies of retinal diseases that are linked to mitochondrial dysfunction.
CircANKRD12 Is Induced in Endothelial Cell Response to Oxidative Stress
Authors: Voellenkle Christine , Fuschi Paola , Mutoli Martina , Carrara Matteo , Righini Paolo , Nano Giovanni , Gaetano Carlo , Martelli Fabio
Published: November 2022

Redox imbalance of the endothelial cells (ECs) plays a causative role in a variety of cardiovascular diseases. In order to better understand the molecular mechanisms of the endothelial response to oxidative stress, the involvement of circular RNAs (circRNAs) was investigated. CircRNAs are RNA species generated by a “back-splicing” event, which is the covalent linking of the 3′- and 5′-ends of exons. Bioinformatics analysis of the transcriptomic landscape of human ECs exposed to H2O2 allowed us to identify a subset of highly expressed circRNAs compared to their linear RNA counterparts, suggesting a potential biological relevance. Specifically, circular Ankyrin Repeat Domain 12 (circANKRD12), derived from the junction of exon 2 and exon 8 of the ANKRD12 gene (hsa_circ_0000826), was significantly induced in H2O2-treated ECs. Conversely, the linear RNA isoform of ANKRD12 was not modulated. An increased circular-to-linear ratio of ANKRD12 was also observed in cultured ECs exposed to hypoxia and in skeletal muscle biopsies of patients affected by critical limb ischemia (CLI), two conditions associated with redox imbalance and oxidative stress. The functional relevance of circANKRD12 was shown by the inhibition of EC formation of capillary-like structures upon silencing of the circular but not of the linear isoform of ANKRD12. Bioinformatics analysis of the circANKRD12–miRNA–mRNA regulatory network in H2O2-treated ECs identified the enrichment of the p53 and Foxo signaling pathways, both crucial in the cellular response to redox imbalance. In keeping with the antiproliferative action of the p53 pathway, circANKRD12 silencing inhibited EC proliferation. In conclusion, this study indicates circANKRD12 as an important player in ECs exposed to oxidative stress.
Epigenetic Changes within the Annulus Fibrosus by DNA Methylation in Rat Intervertebral Disc Degeneration Model
Authors: Hong Jin Young , Kim Hyunseong , Jeon Wan-Jin , Lee Junseon , Yeo Changhwan , Lee Yoon Jae , Ha In-Hyuk
Published: November 2022

Intervertebral disc degeneration (IDD) is an age-dependent progressive spinal disease that causes chronic back or neck pain. Although aging has long been presented as the main risk factor, the exact cause is not fully known. DNA methylation is associated with chronic pain, suggesting that epigenetic modulation may ameliorate disc degeneration. We examined histological changes in the DNA methylation within the discs and their association with pain-related transient receptor potential vanilloid subtype 1 (TrpV1) expression in rats subjected to IDD. Epigenetic markers (5-hydroxymethylcytosine (5hmC), 5-methylcytosine (5Mc)), DNA methyltransferases (DNMTs), and Ten-eleven translocations (Tets) were analyzed using immunohistochemistry, real-time PCR, and DNA dot-blot following IDD. Results revealed high 5mC levels in the annulus fibrosus (AF) region within the disc after IDD and an association with TrpV1 expression. DNMT1 is mainly involved in 5mC conversion in degenerated discs. However, 5hmC levels did not differ between groups. A degenerated disc can lead to locomotor defects as assessed by ladder and tail suspension tests, no pain signals in the von Frey test, upregulated matrix metalloproteinase-3, and downregulated aggrecan levels within the disc. Thus, we found that the DNA methylation status in the AF region of the disc was mainly changed after IDD and associated with aberrant TrpV1 expression in degenerated discs.
RgIA4 Prevention of Acute Oxaliplatin-Induced Cold Allodynia Requires α9-Containing Nicotinic Acetylcholine Receptors and CD3+ T-Cells
Authors: Huynh Peter N. , Christensen Sean B. , McIntosh J. Michael
Published: November 2022

Chemotherapy-induced neuropathic pain is a debilitating and dose-limiting side effect. Oxaliplatin is a third-generation platinum and antineoplastic compound that is commonly used to treat colorectal cancer and commonly yields neuropathic side effects. Available drugs such as duloxetine provide only modest benefits against oxaliplatin-induced neuropathy. A particularly disruptive symptom of oxaliplatin is painful cold sensitivity, known as cold allodynia. Previous studies of the Conus regius peptide, RgIA, and its analogs have demonstrated relief from oxaliplatin-induced cold allodynia, yielding improvement that persists even after treatment cessation. Moreover, underlying inflammatory and neuronal protection were shown at the cellular level in chronic constriction nerve injury models, consistent with disease-modifying effects. Despite these promising preclinical outcomes, the underlying molecular mechanism of action of RgIA4 remains an area of active investigation. This study aimed to determine the necessity of the α9 nAChR subunit and potential T-cell mechanisms in RgIA4 efficacy against acute oxaliplatin-induced cold allodynia. A single dose of oxaliplatin (10 mg/kg) was utilized followed by four daily doses of RgIA4. Subcutaneous administration of RgIA4 (40 µg/kg) prevented cold allodynia in wildtype mice but not in mice lacking the α9 nAChR-encoding gene, chrna9. RgIA4 also failed to reverse allodynia in mice depleted of CD3+ T-cells. In wildtype mice treated with oxaliplatin, quantitated circulating T-cells remained unaffected by RgIA4. Together, these results show that RgIA4 requires both chrna9 and CD3+ T-cells to exert its protective effects against acute cold-allodynia produced by oxaliplatin.
Improving Body Mass Index of School-Aged Children Using a Nine-Week Rope Skipping Training Intervention: A One-Group Pre-Test Post-Test Design
Authors: Frimpong James Boadu , Agyei Michael , Apaak Daniel , Ansah Edward Wilson , True Larissa
Published: November 2022

Studies examining the effectiveness of rope skipping training to improve the body mass index (BMI) of school children are scarce. Hence, this study examined the effectiveness of nine-week skipping training on the BMI of primary six school-aged children (n = 77). The participants underwent 30 min of skipping training three days per week over a nine-week period. The participants’ BMI was measured at the baseline and during weeks 3, 6 and 9. The results from a one-way ANOVA with repeated measures indicated a statistically significant difference in the BMI for both males [F(3,111) = 9.42, p < 0.001, ηp2 = 0.203] and females [F(3,114) = 7.35, p < 0.001, ηp2 = 0.162], suggesting an improvement in BMI. Post hoc comparisons with a Bonferroni adjustment revealed significant differences in BMI after nine weeks of intervention for males between the pre-test (M = 21.47, SD = 4.94) and the 9-week post-test (M = 20.15, SD = 4.36), and for females between the pre-test (M = 21.56, SD = 5.80) and the 9-week post-test (M = 20.68, SD = 5.32). This study demonstrated that regular participation in vigorous physical activity such as skipping training could promote child health by preventing the likelihood of young children being overweight. This result has implications for the inclusion of skipping training into the school life of school-aged children to help manage their BMI levels.
Maternal Multiple Sclerosis and Offspring’s Cognitive and Behavioral Development: What Do We Know until Now?
Authors: Siracusano Martina , Carloni Elisa , Riccioni Assia , Ferrara Marialaura , Scoppola Chiara , Arturi Lucrezia , Niolu Cinzia , Marfia Girolama Alessandra , Mazzone Luigi
Published: November 2022

Multiple Sclerosis (MS) is a chronic pathological condition representing one of the main causes of neurological disability in the female young population. MS, as an immune disorder, could impact fetus development, and, considering the need for and the possibility of pharmacological treatment during pregnancy, the possible influence of medication on developmental trajectories represents a topic of great interest. We provide an overview of the available literature on the influence of maternal Multiple Sclerosis on offspring cognitive and behavioral development. A study was conducted on Pubmed, Medline and Google Scholar, considering empirical studies and reviews exclusively in the English language. Maternal MS appears not to be associated with emotional and behavioral problems, as evaluated through retrospective studies. However, a specific cognitive and behavioral phenotype, through the administration of standardized instruments, has not been delineated yet. Available studies on the topic are characterized by poor methodology and do not lead to conclusions. This overview highlights implications for further longitudinal studies which should delineate offspring developmental trajectories, taking into consideration maternal confounding factors and the exposure to pharmacological treatment in pregnancy.
Impact of COVID-19 Pandemic and Lockdown on the Epidemiology of RSV-Mediated Bronchiolitis: Experience from Our Centre
Authors: Manti Sara , Giallongo Alessandro , Parisi Giuseppe Fabio , Papale Maria , Presti Santiago , Lo Bianco Manuela , Spicuzza Lucia , Leonardi Salvatore
Published: November 2022

Background: The COVID-19 pandemic has dramatically affected the global epidemiology of other infectious respiratory diseases, leading to a significant decrease in their incidence. Hence, we aimed to characterize the epidemiology of RSV-bronchiolitis in children. Methods: children aged ≤2 years diagnosed with RSV-mediated bronchiolitis admitted to our Unit from October 2018 to December 2021, were retrospectively enrolled. Results: We included 95 patients (M/F = 46/49; mean age 7.56 ± 6.6 months). Specifically, 17 infants in 2018, 34 in 2019, 0 during 2020 lockdown, 1 during 2020 post-lockdown, and 43 in 2021. Incidence was significantly lower in 2020 compared with 2018, 2019 and 2021 (p < 0.05). No differences were found concerning need for respiratory support. Discussion: Several factors related to SARS-CoV-2 pandemic, especially restrictive measures, may have contributed to a significant reduction in hospitalizations due to RSV. The new outbreak in RSV infection-related hospitalizations reported between October and December 2021 has been suggested it may be due to an increased number of susceptible individuals to RSV infection. Conclusion: The experience of the SARS-CoV-2 outbreak has led to a marked decrease in other viral respiratory infections, such as RSV. This may pave the way for new approaches in preventing respiratory infections, highlighting the role of preventive measures.
Five-Year Retrospective Study of Uterine STUMP and Leiomyosarcoma
Authors: Bosoteanu Madalina , Deacu Mariana , Voda Raluca Ioana , Orasanu Cristian Ionut , Aschie Mariana , Vlad Sabina Elena , Penciu Roxana Cleopatra , Chirila Sergiu Ioachim
Published: November 2022

Taking into account the unpredictable evolution of uterine STUMP and leiomyosarcomas, there are no clearly established therapeutic protocols to date, the only certified treatment being total hysterectomy. We performed a 5-year retrospective study including cases of malignant tumors and those with uncertain malignant potential originating in the smooth muscle tissue of the uterine body. The clinical data, pathological aspects, and the immunohistochemical results were statistically analyzed using SPSS Statistics Version 26. The main associations of the p53 gene were identified with age, atypia, and the occurrence of metastases. The average number of CD8+ T cells correlated with the hormonal status of the patients, the presence of diabetes, and alteration of thyroid function, but also with the severity of the atypia. The therapeutic method was represented by total hysterectomy, and 30% of the patients with leiomyosarcoma also benefited from adjuvant chemotherapy. The average period until death was 25.66 months. The present study showed that the mutant expression of p53 could have a role in assessing the clinical evolution of patients, given the association with exitus and metastasis. In addition, the average number of CD8+ T cells corresponded to severe atypia, indicating the possibility of applying targeted immunotherapies in these cases.
The Effects of Isotretinoin on The Menstrual Cycle: A Cross-Sectional Study
Authors: Alhetheli Ghadah , Alhazmi Sadin , Almutairi Shumukh , Alharbi Samar , Alharbi Norah , Alsweed Maha , Al-Dhubaibi Mohammed Saleh , Alsaud Jolan , Asiri Lina
Published: November 2022

Menstrual irregularities during isotretinoin therapy, including amenorrhea, can cause a great deal of health-status uncertainty such as the possibility of pregnancy. This study aimed to evaluate the effects of isotretinoin treatment on the menstrual cycle. This cross-sectional study was conducted among females aged between 15–45 years taking isotretinoin for acne. Descriptive statistics were used in the form of frequencies and percentages to represent categorical variables. Pearson’s chi-squared test was performed to assess the relationship between some of the variables with menstrual irregularities. A logistic regression model was performed to assess the risk factors for developing menstrual irregularities during isotretinoin therapy. Of participants with a known regular menstrual cycle, 10.4% were found to have irregularity in their cycle after starting the drug (p < 0.001). Amenorrhea was the most commonly reported menstrual irregularity in isotretinoin-treated females. Our results showed that single females, those who took isotretinoin for 10–12 months and who were concurrently taking hormonal contraceptives all have a statistically significant higher risk of developing menstrual irregularities than others. In conclusion, we found that a statistically significant number of participants with a regular menstrual cycle pre-isotretinoin intake developed irregularity in their cycle after starting the drug. The mechanism of how isotretinoin influences female hormonal imbalances, thereby affecting menstrual irregularities is still poorly understood and needs to be clarified in further clinical studies.
A Semi-Supervised Inspection Approach of Textured Surface Defects under Limited Labeled Samples
Authors: He Yu , Wen Xin , Xu Jing
Published: November 2022

Defect inspection is a key step in guaranteeing the surface quality of industrial products. Based on deep learning (DL) techniques, related methods are highly effective in defect classification tasks via a supervision process. However, collecting and labeling many defect samples are usually harsh and time-consuming processes, limiting the application of these supervised classifiers on various textured surfaces. This study proposes a semi-supervised framework, based on a generative adversarial network (GAN) and a convolutional neural network (CNN), to classify defects of a textured surface, while a novel label assignment scheme is proposed to integrate unlabeled samples into semi-supervised learning to enhance the overall performance of the system. In this framework, a customized GAN uses limited labeled samples to generate unlabeled ones, while the proposed label assignment scheme makes the generated data follow different label distributions in such a way that they can participate in training with labeled data. Finally, a CNN is proposed for semi-supervised training and the category identification of each defect sample. Experimental results show the effectiveness and robustness of the proposed framework even if original samples are limited. We verify our approach on four different surface defect datasets, achieving consistently competitive performances.
Preparation and Properties of Epoxy Resin and Polyurethane Blend Resin-Based Low-Infrared-Emissivity Coatings
Authors: Zhang Jiaming , Zhang Weigang , Guan Qishuai , Li Xiang , Lv Dandan
Published: November 2022

A low-infrared-emissivity composite coating with outstanding mechanical properties and low glossiness was prepared by using an epoxy resin and polyurethane blend modified resin, flake brass powder, the silane coupling agent KH560, and a dispersant as an adhesive, a functional pigment, and interface modifiers, respectively. The effects of the mass ratios of epoxy resin and polyurethane, the addition amount of flake brass powder, the addition amount of KH560, and the addition amount of dispersant on the coating performance were systematically discussed. The results show that the coating has lower emissivity (0.424) and glossiness (28.8) when the mass ratio of epoxy resin to polyurethane is 6:4 and the addition amount of brass powder is 50 wt%. Using KH560 to modify the interface structure of the coating, KH560 can form chemical bond with the resin matrix and flake brass powder so as to obviously improve the impact strength and flexibility of the coating. The addition of the dispersant can obviously enhance the dispersion state of the flake brass powder in the coating and thus enhance the reflection intensity of infrared light for the coating, such that the emissivity of the coating is obviously reduced. When the addition amount of KH560 is 7 wt% and the addition amount of the dispersant is 9 wt%, the coating has the optimal emissivity (0.332), glossiness (9.0), adhesion strength (grade 1), impact strength (50 kg·cm), and flexibility (1 mm) at the same time. The above mentioned low-infrared-emissivity coating has an important application prospect in the field of infrared stealth for various aircraft.
Investigating the Synergic Effects of WS2 and ECAP on Degradation Behavior of AZ91 Magnesium Alloy
Authors: Abbas Aqeel , Huang Song-Jeng
Published: November 2022

In this research, WS2/AZ91 metal matrix composites were manufactured using the stir casting method. The composites were severely deformed using equal channel angular pressing (ECAP). The degradation behavior of severely deformed and as-cast samples was investigated using the three-electrode system in a 3.5 wt% NaCl solution. The corrosion products and surface morphology of the corroded surfaces were investigated using an x-ray diffractometer and scanning electron microscopy. The results revealed that as-cast monolithic AZ91 exhibited the highest corrosion potential of (−1.553 mV) and a minimum degradation rate (4.099 m·a−1). The reduction ion grain size after severe plastic deformation increased the degradability of WS2/AZ91 composites. Severe plastic deformation reduced the grain size, which led to an increase in the corrosion rate. The synergic effects of (tungsten disulfide) WS2 and ECAP increased the degradation rate to (4.59 m·a−1) in two-pass 1 wt% WS2/AZ91. The increase in WS2 contents decreased the degradation rate (4.512 m·a−1) in homogenized 1 wt% WS2/AZ91 composites. The degradability of AZ91 increased in all conditions under the synergic effects of WS2 and severe plastic deformation. Magnesium and magnesium hydroxide were observed as corrosion products. The maximum surface roughness in two-pass 1 wt% WS2/AZ91 indicated the highest material removed by corrosion from the surface of the composites.
Tunable Electrical Properties of Ti-B-N Thin Films Sputter-Deposited by the Reactive Gas Pulsing Process
Authors: Sakkas Charalampos , Cote Jean-Marc , Gavoille Joseph , Rauch Jean-Yves , Cornuault Pierre-Henri , Krystianiak Anna , Heintz Olivier , Martin Nicolas
Published: November 2022

Titanium-boron-nitrogen (Ti-B-N) thin films were deposited by RF reactive magnetron sputtering using a titanium diboride (TiB2) target in an argon + nitrogen mixture. The argon mass flow rate was kept constant, whereas that of nitrogen was pulsed during the deposition. A constant pulsing period of P = 10 s was used, and the introduction time of the nitrogen gas (duty cycle (dc)) was systematically varied from dc = 0 to 100% of the pulsing period. This reactive gas pulsing process allowed the deposition of Ti-B-N thin films with various boron and nitrogen concentrations. Such adjustable concentrations in the films also led to changes in their electronic transport properties. Boron and nitrogen contents exhibited a reverse evolution as a function of the nitrogen duty cycle, which was correlated with the transition from a metallic to semiconducting-like behavior. A percolation model was applied to the electrical conductivity as a function of the nitrogen pulsing parameters, assuming some correlations with the evolution of the Ti-B-N thin film nanostructure.
Thermoresponsive Starch Nanoparticles for the Extraction of Bitumen from Oil Sands
Authors: Dasgupta Natun , Wang Jun-Zhi Oliver , Nguyen Vo Thu An , Gauthier Mario
Published: November 2022

Starch nanoparticles (SNPs) useful for the extraction of bitumen from oil sands were obtained by modification with thermoresponsive poly(di(ethylene glycol) methyl ether methacrylate) (PMEO2MA) segments through RAFT (Reversible Addition–Fragmentation chain Transfer) grafting. Since PMEO2MA exhibits a Lower Critical Aggregation Temperature (LCAT), the polymer-grafted SNPs are amphiphilic above the LCAT of the thermoresponsive polymer and can interact efficiently with bitumen in the oil sands, facilitating its extraction. The PMEO2MA-grafted SNPs form micellar aggregates that remain dispersed in water but can shuttle the bitumen component out of the sand and silt mixture in the extraction process above the LCAT. Upon cooling, the hydrophobic PMEO2MA domains become hydrophilic again and the grafted SNPs remain in the water phase, while the extracted oil floats on the aqueous phase and can be skimmed off. The aqueous polymer solution may be reused in other extraction cycles. Extraction by tumbling of the oil-water-SNP mixtures in vials at 45 °C reached over 80% efficiency. The synthetic methods used provide easy control over the characteristics of the grafted SNPs (number and length of grafted PMEO2MA segments), and therefore over their hydrophilic-lipophilic balance (HLB). The SNP-g-PMEO2MA samples were characterized by 1H NMR, UV-visible spectroscopy and dynamic light scattering analysis, and the grafted PMEO2MA chains were cleaved from the starch substrates for analysis by gel permeation chromatography.
Frequency of Positive Results for SARS-CoV-2 Infection in Symptomatic Pediatric Patients Treated in the Emergency Department of a Regional Hospital
Authors: López Gabriela Fernanda , Fenollosa Andreu , Wolter Victoria , Satorra Pau , Pérez-López Carlos , Rodríguez-Molinero Alejandro
Published: November 2022

Objectives: Since 11 March 2020, when the World Health Organization declared the COVID-19 pandemic, trials have found that the pediatric population is the least affected, and most positive cases are asymptomatic or have mild symptoms such as fever and cough. Aims: The main objective of this study was to describe the percentage of positive tests among children who presented to the emergency department for symptoms compatible with SARS-CoV-2. Additional objectives were to identify clinical screening criteria to identify candidates to perform a polymerase chain reaction or antigen screening test. Patients/Methods: The study had a mixed cross-sectional and case-control design. Data were obtained from the electronic medical records of the emergency service of Hospital Sant Camil, Sant Pere de Ribes (Spain), from May 2020 to September 2021. Patients included had symptoms compatible with COVID-19 and a positive polymerase chain reaction or antigen SARS-CoV-2 screening test. Results: The study included 1508 patients with a mean age of 4.5 years (SD 4.2): 670 of whom were female (44.4%). The percentage of children with a positive polymerase chain reaction or antigen test was 3.51%. For the secondary objectives, some rules were created to create groups in which all patients tested negative: (1) Patients with abdominal pain, who did not present with diarrhea or fatigue, (2) Patients with asthma or allergies, (3) patients older than 3.5 years, with asthenia but without abdominal pain, and (4) patients younger than 3.5 years with abdominal pain and rash. Conclusions: The frequency of children who tested positive for COVID-19 was low among those with a clinical suspicion tested in the emergency room. Some combinations of symptoms and personal history were without exception associated with a negative polymerase chain reaction result for SARS-CoV-2 and could therefore help rule out the disease.
Investigation of the Effect of ITO Size and Mesa Shape on the Optoelectronic Properties of GaN-Based Micro LEDs
Authors: Fang Aoqi , Xu Hao , Guo Weiling , Liu Jixin , Chen Jiaxin , Li Mengmei
Published: November 2022

In this paper, in order to explore the influence of indium tin oxide (ITO) size and mesa shape on the performance of GaN-based micro light emitting diodes (Micro LEDs) on sapphire substrates, Micro LEDs of different sizes with ITO area smaller than or equal to the light-emitting area were designed and fabricated. The experiment results show that when the ITO area of the Micro LED is equal to the area of the light-emitting area, its optoelectronic performance is significantly better than that of the Micro LEDs whose ITO area is smaller than the area of the light-emitting area. When the light-emitting area size is 40 μm, the wall-plug efficiency (WPE) of the two structures of Micro LEDs can differ by more than 50%. Based on above experiment results, this paper designed and fabricated Micro LEDs with different sizes of square and circular mesa with the same ITO area as the light-emitting area. The experimental results show that the WPE of the circular mesa Micro LED is slightly higher than that of the square mesa Micro LED at low current density. However, as the current density and chip size increase, the performance of the Micro LED with a square mesa is better.
Reference-Guided Draft Genome Assembly, Annotation and SSR Mining Data of the Peruvian Creole Cattle (Bos taurus)
Authors: Estrada Richard , Corredor Flor-Anita , Figueroa Deyanira , Salazar Wilian , Quilcate Carlos , Vásquez Héctor V. , Maicelo Jorge L. , Gonzales Jhony , Arbizu Carlos I.
Published: November 2022

The Peruvian creole cattle (PCC) is a neglected breed and an essential livestock resource in the Andean region of Peru. To develop a modern breeding program and conservation strategies for the PCC, a better understanding of the genetics of this breed is needed. We sequenced the whole genome of the PCC using a de novo assembly approach with a paired-end 150 strategy on the Illumina HiSeq 2500 platform, obtaining 320 GB of sequencing data. A reference scaffolding was used to improve the draft genome. The obtained genome size of the PCC was 2.81 Gb with a contig N50 of 108 Mb and 92.59% complete BUSCOs. This genome size is similar to the genome references of Bos taurus and B. indicus. In addition, we identified 40.22% of repetitive DNA of the genome assembly, of which retroelements occupy 32.39% of the total genome. A total of 19,803 protein-coding genes were annotated in the PCC genome. For SSR data mining, we detected similar statistics in comparison with other breeds. The PCC genome will contribute to a better understanding of the genetics of this species and its adaptation to tough conditions in the Andean ecosystem.
AAL and Internet of Medical Things for Monitoring Type-2 Diabetic Patients
Authors: Ahmed Shakeel , Srinivasu Parvathaneni , Alhumam Abdulaziz , Alarfaj Mohammed
Published: November 2022

Due to an aging population, assisted-care options are required so that senior citizens may maintain their independence at home for a longer time and rely less on caretakers. Ambient Assisted Living (AAL) encourages the creation of solutions that can help to optimize the environment for senior citizens with assistance while greatly reducing their challenges. A framework based on the Internet of Medical Things (IoMT) is used in the current study for the implementation of AAL technology to help patients with Type-2 diabetes. A glucose oxide sensor is used to monitor diabetic elderly people continuously. Spectrogram images are created from the recorded data from the sensor to assess and detect aberrant glucose levels. DenseNet-169 examines and analyzes the spectrogram pictures, and messages are sent to caregivers when aberrant glucose levels are detected. The current work describes both the spectrogram image analysis and the signal-to-spectrogram generating method. The study presents a future perspective model for a mobile application for real-time patient monitoring. Benchmark metrics evaluate the application’s performances, including sensitivity, specificity, accuracy, and F1-score. Several cross--validations are used to evaluate the model’s performance. The findings demonstrate that the proposed model can correctly identify patients with abnormal blood glucose levels.
Accuracy of Diagnostic Tests for the Detection of Chagas Disease: A Systematic Review and Meta-Analysis
Authors: Candia-Puma Mayron Antonio , Machaca-Luque Laura Yesenia , Roque-Pumahuanca Brychs Milagros , Galdino Alexsandro Sobreira , Giunchetti Rodolfo Cordeiro , Coelho Eduardo Antonio Ferraz , Chávez-Fumagalli Miguel Angel
Published: November 2022

The present systematic review and meta-analysis about the accuracy of diagnostic tests aim to describe the findings of literature over the last thirty years for the diagnosis of Chagas disease (CD). This work aimed to determine the accuracy of diagnostic techniques for CD in the disease’s acute and chronic phases. The PubMed database was searched for studies published between 1990 and 2021 on CD diagnostics. Fifty-six published studies that met the criteria were analyzed and included in the meta-analysis, evaluating diagnostic accuracy through sensitivity and specificity. For Enzyme-Linked Immunosorbent Assay (ELISA), Fluorescent Antibody Technique (IFAT), Hemagglutination Test (HmT), Polymerase Chain Reaction (PCR), and Real-Time Polymerase Chain Reaction (qPCR) diagnosis methods, the sensitivity had a median of 99.0%, 78.0%, 75.0%, 76.0%, and 94.0%, respectively; while specificity presented a median of 99.0%, 99.0%, 99.0%, 98.0%, and 98.0%, respectively. This meta-analysis showed that ELISA and qPCR techniques had a higher performance compared to other methods of diagnosing CD in the chronic and acute phases, respectively. It was concluded utilizing the Area Under the Curve restricted to the false positive rates (AUCFPR), that the ELISA diagnostic test presents the highest performance in diagnosing acute and chronic CD, compared to serological and molecular tests. Future studies focusing on new CD diagnostics approaches should be targeted.
The Added Value of Intraventricular Hemorrhage on the Radiomics Analysis for the Prediction of Hematoma Expansion of Spontaneous Intracerebral Hemorrhage
Authors: Wu Te-Chang , Liu Yan-Lin , Chen Jeon-Hor , Zhang Yang , Chen Tai-Yuan , Ko Ching-Chung , Su Min-Ying
Published: November 2022

Background: Among patients undergoing head computed tomography (CT) scans within 3 h of spontaneous intracerebral hemorrhage (sICH), 28% to 38% have hematoma expansion (HE) on follow-up CT. This study aimed to predict HE using radiomics analysis and investigate the impact of intraventricular hemorrhage (IVH) compared with the conventional approach based on intraparenchymal hemorrhage (IPH) alone. Methods: This retrospective study enrolled 127 patients with baseline and follow-up non-contrast CT (NCCT) within 4~72 h of sICH. IPH and IVH were outlined separately for performing radiomics analysis. HE was defined as an absolute hematoma growth > 6 mL or percentage growth > 33% of either IPH (HEP) or a combination of IPH and IVH (HEP+V) at follow-up. Radiomic features were extracted using PyRadiomics, and then the support vector machine (SVM) was used to build the classification model. For each case, a radiomics score was generated to indicate the probability of HE. Results: There were 57 (44.9%) HEP and 70 (55.1%) non-HEP based on IPH alone, and 58 (45.7%) HEP+V and 69 (54.3%) non-HEP+V based on IPH + IVH. The majority (>94%) of HE patients had poor early outcomes (death or modified Rankin Scale > 3 at discharge). The radiomics model built using baseline IPH to predict HEP (RMP) showed 76.4% accuracy and 0.73 area under the ROC curve (AUC). The other model using IPH + IVH to predict HEP+V (RMP+V) had higher accuracy (81.9%) with AUC = 0.80, and this model could predict poor outcomes. The sensitivity/specificity of RMP and RMP+V for HE prediction were 71.9%/80.0% and 79.3%/84.1%, respectively. Conclusion: The proposed radiomics approach with additional IVH information can improve the accuracy in prediction of HE, which is associated with poor clinical outcomes. A reliable radiomics model may provide a robust tool to help manage ICH patients and to enroll high-risk ICH cases into anti-expansion or neuroprotection drug trials.
“I Feel Proud That with This App We Can Help Other People with Intellectual Disabilities”: The Role of an Expert by Experience Digital Inclusion Citizen Advisory Panel
Authors: Murphy Esther , Shiels Orla , Yap Mei Lin , Angus Fionn Crombie , Delaney James , Hogan Brian , Burke Christina , Heduan Darren , Breslin Denise , Fiori Sara
Published: November 2022

Despite legislation affirming the rights of people with intellectual disabilities to take equal part in society, marginalization persists. Accessibility needs can impact this group’s ability to engage in the digital society and in aspects of daily living, such as employment and socializing. The Digi-ID PLUS study was established with a team of seven people with an intellectual disability. Team members were hired as a diverse group to give insight into their lived experiences in reviewing and validating research findings. Their insights were key to every aspect of Digi-ID PLUS. It has been recognized that user-centered design enhances technology development and accessibility; therefore, the aim of establishing a Citizen Advisory Panel (CAP) in our program design and delivery is to include the critical role of users by experience to review, validate and test our solution to enhance the accessibility of all aspects of the program. Working with Digi-ID PLUS, the CAP spoke of the impact being paid team members had on their lives. Analysis of insights shared indicated that being a part of the team had a positive impact on their self-image confidence and digital skills acquisition CAP members discussed the importance of supporting each other and the value of being paid for their insights.
Oral Mucosal Lesions in Childhood
Authors: Horvat Aleksijević Lorena , Prpić Jelena , Muhvić Urek Miranda , Pezelj-Ribarić Sonja , Ivančić-Jokić Nataša , Peršić Bukmir Romana , Aleksijević Marko , Glažar Irena
Published: November 2022

Childhood diseases are a continuous source of interest in all areas of general and dental medicine. Congenital, developmental, and hereditary diseases may either be present upon birth or appear in early childhood. Developmental anomalies, although often asymptomatic, may become grounds for different infections. Furthermore, they can indicate certain systemic disorders. Childhood age frequently brings about benign tumors and different types of traumatic lesions to the oral mucosa. Traumatic lesions can be caused by chemical, mechanical, or thermal injury. Mucocele and ranula are, by definition, traumatic injuries of the salivary glands or their ducts. Recurrent aphthous lesions are the most common type of ulcerations in childhood, and their etiology is considered multifactorial. Oral mucosal lesions in children require different treatment approaches depending on etiological factors and clinical presentation. Clinicians should have adequate knowledge of oral anatomy in order to diagnose and treat pathological conditions.
Fit, Precision, and Trueness of 3D-Printed Zirconia Crowns Compared to Milled Counterparts
Authors: Abualsaud Reem , Alalawi Haidar
Published: November 2022

Precise fit of a crown and accurate reproduction of the digital design are paramount for successful treatment outcomes and preservation of clinician and technician time. The study aimed to compare the internal fit, marginal adaptation, precision, and trueness of 3D-printed zirconia crowns compared to their milled counterpart. A total of 20 monolithic 3 mol% yttria stabilized-zirconia crowns (n = 10) were made using computer-assisted design (CAD) followed by additive (3D-printed) and subtractive (milled) manufacturing. Digital scanning of the master die with and without a fit checker followed by image superimposition, and analysis was performed to evaluate internal and marginal adaptation in four areas (occlusal, axial, marginal, and overall). ISO 12836:2015 standard was followed for precision and trueness evaluation. Statistical analysis was achieved using a t-test at α = 0.05. Internal fit and marginal adaptation revealed no significant difference between the two test groups (p > 0.05). The significant difference in trueness (p < 0.05) was found between the two groups in three areas (occlusal, axial, and internal). The best and worst trueness values were seen with 3D-printed crowns at occlusal (8.77 ± 0.89 µm) and Intaglio (23.90 ± 1.60 µm), respectively. The overall precision was statistically better (p < 0.05) in the 3D-printed crowns (9.59 ± 0.75 µm) than the milled (17.31 ± 3.39 µm). 3D-printed and milled zirconia crowns were comparable to each other in terms of internal fit and marginal adaptation. The trueness of the occlusal and axial surfaces of 3D-printed crowns was better, whereas the trueness of fitting surface of milled crowns was better. 3D-printed crowns provided a higher level of precision than milled crowns. Although the internal and marginal fit of both production techniques were comparable, 3D printing of zirconia produced more precise crowns.