bluuebunny/crossref_metadata_2025 huggingface dataset:
Single Trip Curve-Lateral Drilling with High Dogleg RSS
Authors: Tilley Jim , Demirer Nazli , Bond Paul , Penman Andrew , Hamoudi Lotfi , Della Chiesa David
Published: March 2021

This paper discusses the design of an attitude controller embedded in a high dogleg, fully-rotating rotary steerable system (RSS). While drilling with a high dogleg capable RSS at high rate of penetration (ROP), directional control may become challenging along tangent sections, thus, making it difficult to drill vertical, curve, and lateral sections within a single trip with low tortuosity. To ensure tight inclination and azimuth control along tangent sections with high dogleg RSS, we introduce an on-board robust attitude control system and discuss its performance over several curve-lateral case studies drilled at high ROPs.

The downhole navigation and control system consists of a multiplicity of accelerometers, a magnetometer, and gyroscopes that are used to precisely measure borehole attitude via sophisticated sensor fusion algorithms. The attitude measurements representing borehole direction in a three-dimensional frame are fed back to the controller, which communicates a corrective control command to a high-bandwidth actuation system to minimize deviation from the target attitude. This corrective control command is generated by an advanced control method that is optimized to provide good attitude tracking performance and necessary robustness against potential formation pushes during high ROP drilling.

Several case histories from wells drilled using the automatic attitude control feature of the high dogleg RSS will be shared in the paper and discussed in detail. The cases include single-trip curve-lateral runs from onshore North America (Marcellus Shale) drilled at high ROPs. The entirety of the lateral sections of these wells, each longer than 10,000 ft, are drilled with the automatic attitude control mode. While generating consistently over 12°/100ft dogleg severity in curve sections, the RSS was able to drill smooth lateral sections with an average of over 200 ft/hr ROP and by keeping the average dogleg severity at approximately 1.2°/100ft.

The attitude-hold controller enables the high dogleg capable RSS to drill smooth vertical sections, tangents, and extended laterals, along with the curve sections, in just a single run. This technology has the potential to offer cost savings on several fronts: (1) reducing non-drilling time due to tripping in and out between different sections of the well; (2) allowing for drilling at an increased ROP by maintaining tight directional control and, therefore, reducing the drilling time; and (3) increasing operational efficiency by shifting the steering decision-making process to the downhole given target attitude.
PeptiCHIP: a novel microfluidic-based chip platform for tumour antigen landscape identification
Authors: Feola Sara , Haapala Markus , Peltonen Karita , Capasso Cristian , Martins Beatriz , Antignani Gabriella , Federico Antonio , Pietiäinen Vilja , Chiaro Jacopo , Feodoroff Michaela , Rannikko Antti , Fusciello Manlio , Koskela Satu , Partanen Jukka , Hamdan Firas , Tähkä Sari , Ylösmäki Erkko , Greco Dario , Grönholm Mikaela , Kekarainen Tuija , Eshaghi Masoumeh , Gurvich Olga L. , Ylä-Herttuala Seppo , Branca Rui Mamede , Lehtiö Janne , Sikanen Tiina , Cerullo Vincenzo
Published: March 2021

Identification of HLA class I ligands from the tumour surface (ligandome or immunopeptidome) is essential for designing T-cell mediated cancer therapeutic approaches. However, the sensitivity of the process for isolating MHC-I restricted tumor-specific peptides has been the major limiting factor for reliable tumor antigen characterization, making clear the need for technical improvement.
Here, we describe our work from the fabrication and development of a novel microfluidic-based chip (PeptiCHIP) and to its use to identify and characterize tumor-speficic ligands on clinically relevant human organoids. Specifically, we assessed the potential of immobilizing a pan-HLA antibody on solid surfaces via well-characterized streptavidin-biotin chemistry, overcoming the limitations of the cross-linking chemistry used to prepare the affinity matrix with the desired antibodies in the immunopeptidomics workflow. Furthermore, to address the restrictions related to the handling and the limited availability of tumour samples, we further developed the concept towards the implementation of a microfluidic through-flow system.
Thus, the biotinylated pan-HLA antibody was immobilized on streptavidin-functionalized surfaces, and immune-affinity purification (IP) was carried out on customized microfluidic pillar arrays made of thiol-ene polymer. Compared to the standard methods reported in the field, our methodology drastically reduces the handling, the amount of antibody and the time required for peptide isolation.
In this work, we carefully examined the specificity and robustness of our customized technology for immunopeptidomics workflows. We tested this novel platform by immunopurifying HLA-I complexes from as few as 106 cells both in a widely studied B-cell line and in patients-derived ex vivo cell cultures. After the final elution in mild acid, HLA-I-presented peptides were identified by tandem mass spectrometry and further investigated by in vitro methods. These results highlight the potential to exploit microfluidics-based strategies in immunopeptidomics platforms and in personalized immunopeptidome analysis from cells isolated from individual tumour biopsies to design tailored cancer therapeutic vaccines.
Barriers and Facilitators of Art Adherence Among Hiv Positive Patients in Community Client-led Art Delivery Groups at Lira Regional Referral Hospital
Authors: Isabirye Rogers , Puleh Steven Sean , Opii Didan Jacob , Opio-Ekit Sharon , kawomera Alice , Lokiru Luke , Isoke Robert , Ssenkaali Joachim , Kumakech Edward , Obua Celestino
Published: March 2021

Background: The community client-led ART delivery groups (CCLADs) was introduced as one of the strategies to better serve individual needs and reduce unnecessary burdens on the health system. However, no study has comprehensively explained what are the drivers and barriers of CCLADs in improving treatment outcomes. This study sought to assess the barriers and facilitators of ART adherence among HIV positive patients attending CCLADs at Lira Regional Referral Hospital (LRRH), Lira District.Method: We employed a mixed methodology involving 150 study participants between July to August 2020. Quantitative data was obtained from all the participants that were picked through systematic random sampling using a semi-structured questionnaire. Data was entered into SPSS version 23.0 and analyzed at 95% level of significance. We conducted 25 in-depth interviews guided by a checklist. Qualitative data was analyzed through thematic content analysis of major themes that emerged from participants’ responses.Results: Our study found that majority 94.7% (142/150) of the respondents had an optimal adherence (100%) calculated retrospectively based on a 4-day pill uptake recall. In addition, 39.3% (59/150) of participants in CCLADs at LRRH had missed taking a pill in a period of at least 4 weeks. Among all the 104 respondents that had ever missed a medication, the most frequent reason (35% (35/104)) for missing a medication was travelling far away.From qualitative data, social support, patient self-motivation, health education and counselling and guidance were the major facilitators to adherence. On the other hand, lack of food, stigma, forgetfulness, stress, unfair hospital staffs and cultural beliefs were the major perceived barriers to ART adherence.Conclusion/recommendation: Good adherence was attributed to availability of ART at the clinic and an efficient delivery strategy. More health care follow up interventions should be designed to ensure total pill uptake by PLWHIV in communities.
Usefulness of 3D-printed Coplanar Puncture Template Assisted CT- guided Percutaneous Core Needle Biopsy of Small (≤20mm) Pulmonary Nodules: 3 years of experience
Authors: Wang Hansheng , Chen Xiao , Ren Tao , Chen Peipei , Luo Guoshi , Wang Xiao , Wei Na , Tang Yijun , Wang Meifang
Published: March 2021

Background and Objective: Computed tomography guided percutaneous lung biopsy is a commonly used method for clarifying the nature of pulmonary nodules. However, due to the existence of breathing movement, the lungs have greater mobility, and biopsy of small pulmonary nodules is difficult. In recent years, 3D-printed coplanar templates are gradually used in percutaneous biopsy of small pulmonary nodules. Therefore, this study aimed to evaluate the application value of 3D-printed coplanar puncture template assisted computed tomography guided percutaneous core needle biopsy of small (≤20mm) pulmonary nodules.Method: From January 2018 to January 2021 in Taihe Hospital, 210 hospitalized patients with pulmonary nodules underwent percutaneous core needle biopsy for histopathology/cytopathology diagnosis and were included in the study. All patients were allocated into two groups, patients in Free-hand group underwent percutaneous core needle biopsy without 3D-printed coplanar templates, and patients in 3D-PCT group underwent percutaneous core needle biopsy with 3D-printed coplanar templates. The number of needle adjustments, number of CT scans, surgical time-consuming, diagnostic accuracy, and incidence of complications were recorded and compared between the two groups. Rapid on site evaluation was routinely used for guiding specimens’ triage. Approval for this trial was obtained from the Ethics Committee of the Taihe hospital.Result：The number of needle adjustments (1.41±0.63 vs. 2.23±0.85), the number of CT scans (3.63±0.73 vs. 4.25±0.81) and the incidence of pneumothorax (9.6% vs. 21.1%) during the procedure were significantly lower in the observation group than the control group (P< 0.05), whereas there were no significant differences in the diagnostic accuracy (95.2% vs. 94.3%) and incidence of pulmonary hemorrhage (65.4% vs. 62.3%) between the two groups (P> 0.05), however, the surgical time-consuming in 3D-PCT group is significantly more than Free-hand group.Conclusions: These findings indicated that the 3D-printed coplanar puncture template combined with CT guided percutaneous biopsy can relatively fix the target lesion, reduce the number of needle adjustments and number of CT scans, reduce iatrogenic radiation, and reduce the incidence of complications, especially pneumothorax
The different risk factors for isolated diastolic hypertension and isolated systolic hypertension: a national survey
Authors: Xie Kun , Gao Xiufang , Bao Liwen , Shan Ying , Shi Haiming , Li Yong
Published: March 2021

Background

Hypertension is highly prevalent and is one of the modifiable risk factors for cardiovascular outcomes. Isolated diastolic hypertension (IDH), however, tend to be ignored due to insufficient recognition. We sought to depict the clinical manifestation of IDH and isolated systolic hypertension (ISH) in order to find a more efficient way to improve the management.
Methods

Patients with primary hypertension aged over 18 years were investigated from all over the country using convenience sampling during 2017–2019. IDH was defined as systolic blood pressure (SBP) < 140 mmHg and diastolic blood pressure (DBP) ≥ 90 mmHg. ISH was defined as SBP ≥ 140 mmHg and DBP < 90 mmHg.
Results

Totally 8548 patients were screened and
8475 participants were included.

The average age was 63.67 ± 12.78 years and male accounted for 54.4%. Among them, 361 (4.3%) had IDH and 2096 had ISH (24.7%). Patients with IDH (54.84 ± 13.21 years) was much younger. Aging turned out to be negatively associated with IDH but positively associated with ISH. Logistic analysis showed BMI was a significant risk factor for IDH (OR 1.30, 95%CI 1.05–1.61, p = 0.018), but not for ISH (OR 1.05, 95%CI 0.95–1.16, p = 0.358). Moreover, smoking was significantly associated with IDH (OR 1.36, 95%CI 1.04–1.78, p = 0.026) but not with ISH (OR 1.04, 95%CI 0.90–1.21, p = 0.653).
Conclusions

Patients with IDH were much younger and the prevalence decreased with aging. BMI and smoking were remarkably associated with IDH rather than ISH. Keeping fit and giving up smoking might be particularly efficient in the management of young patients with IDH.
Trial registration:

NCT03862183, retrospectively registered on March 5, 2019
Effectiveness of local exercise therapy versus spinal manual therapy in patients with patellofemoral pain syndrome: medium term follow-up results of a randomized controlled trial.
Authors: Scafoglieri Aldo Georges Celine , Broeck Jona Van den , Willems Stijn , Tamminga Rob , Hoeven Henk van der , Engelsma Yde , Haverkamp Stijn
Published: March 2021

Background

Increasing evidence has shown benefits of spinal manipulations in patients with patellofemoral pain syndrome (PFPS). There is scarcity regarding medium term effects of spinal manual therapy on outcome measures in PFPS patients. Therefore, the aim of the present study was to compare the effectiveness of local exercise therapy and spinal manual therapy for knee pain, function and maximum voluntary peak force (MVPF) velocity of the quadriceps.
Methods

Forty-three patients with PFPS were randomly assigned to an intervention group. The local exercise group received six sessions of supervised training of the knee-and hip muscles with mobilization of the patellofemoral joint. The spinal manual therapy group received six interventions of high velocity low thrust manipulations at the thoracolumbar region, sacroiliac joint, and/or hip. Maximum, minimum and current pain were measured using the visual analogue scale. Function was assessed with the anterior knee pain scale (AKPS) and MPFV was recorded using a Biodex System 3 dynamometer. Patients were assessed before intervention, after 6 weeks of intervention and at 6 weeks follow-up.
Results

Pain and functionality improved more following spinal manipulative therapy than local exercise therapy. After 6 weeks of intervention the between-group difference (local versus spinal) for maximal pain was 23.4 mm [95% CI: 9.3, 37.6; effect size (ES): 1.04] and − 12.4 [95% CI: -20.2, -4.7; ES: 1.00] for the AKPS. At 6 weeks of follow-up the between-group difference for maximal pain was 18.7 mm [95% CI: 1.4, 36.0; ES: 0.68] and − 11.5 [95% CI: -19.9, -3.3; ES: -0.87] for the AKPS.
Conclusions

This study suggests that spinal manual therapy is more effective than local exercise therapy in improving pain and function in patients with PFPS in the medium term. Combining spinal manual therapy with local exercise therapy may be an important element in the successful rehabilitation of patients with PFPS.
Long non-coding RNA OIP5-AS1 aggravates acute lung injury through promoting inflammation and cell apoptosis via regulating miR-26a-5p/TLR4 axis
Authors: Sun Qingsong , Luo Man , Gao Zhiwei , Han Xiang , Wu Weiqin , Zhao Hongmei
Published: March 2021

Background: Acute lung injury (ALI) is a pulmonary disorder that leads to acute failure of respiration and thereby results in a high mortality worldwide. Increasing studies have verified that TLR4 is a promoter in ALI, however, the underlying upstream mechanisms of TLR4 was still rarely investigated. Methods: Lipopolysaccharide (LPS) was used to induce cell model and animal model. A wide range of experiments including RT-qPCR, Western blot, ELISA, flow cytometry, H&E staining, RIP, luciferase activity and caspase-3 activity were carried out to figure out the expression status, specific role and potential upstream mechanism of TLR4.Result: RT-qPCR identified that TLR4 expression was upregulated in ALI mice and LPS-induced WI-38 cells. Moreover, miR-26a-5p was confirmed to target TLR4 according to luciferase reporter assay. Besides, miR-26a-5p overexpression decreased the contents of proinflammatory factors (TNF-α and IL-1β) and restrained cell apoptosis, while upregulation of TLR4 reversed these effects of miR-26a-5p mimics, implying that miR-26a-5p alleviated ALI through regulating TLR4. Afterwards, OIP5-AS1 was identified to bind with miR-26a-5p by RNA immunoprecipitation (RIP) and luciferase reporter assay. Functionally, OIP5-AS1 upregulation accelerated the inflammation injuries and miR-26a-5p overexpression counteracted the influence of OIP5-AS1 upregulation on proinflammatory factors and cell apoptosis.Conclusion: OIP5-AS1 accelerated ALI through regulating miR-26a-5p/TLR4 axis in ALI mice and LPS-induced cells, which indicates a promising insight into diagnostics and therapeutics in ALI.
Heavy Metals Bioremediation and Water Softening Using Ureolytic Metschnikowia Pulcherrima and Raoultella Planticola Strains
Authors: Eltarahony Marwa , Kamal Ayman , zaki sahar , Abdelhaleem Desouky
Published: March 2021

This study employed the ureolytic fungal Metschnikowia pulcherrima (29A) and bacterial Raoultella planticola (VIP) strains in Pb 2+ and Hg 2+ removal using the promising CaCO 3 bio precipitation technique. Out of fifty isolates, strains 29A and VIP were selected based on their highest ureolytic activity followed by MIC assay using 350 ppm of Pb 2+ and Hg 2+ . The maximum urease activity recorded 884 and 639 U/mL for 29A and VIP strains at 24 and 30h of incubation, respectively. Complete removal of Pb 2+ was achieved at 42h and 90h for 29A, VIP correspondingly, while Hg 2+ was totally removed at 60h and 102h for 29A, VIP respectively. Remarkable removal of Ca 2+ (>95%) was achieved by the end of the experiments, which would address the hardness problem in the water treatment process. Further, EDX, SEM and, XRD were used to characterize the remediated precipitates. EDX profiles showed characteristic peaks of C, O and, Ca 2+ besides Pb 2+ and Hg 2+ . SEM illustrated the presence of microbial imprints and calcinated cells in the remediated bioliths. However, XRD confirmed the transformation of soluble metals to insoluble forms entrapped in calcite or vaterite lattice. Such a bioremediation approach ensures the detoxification and sequestration of heavy metals in a stable and durable matrix; obstructing their leach from carbonate complex trap to the environment.
A novel strategy for transmural stent removal in necrotizing pancreatitis undergoing endoscopic transmural necrosectomy: a nested, case-control study
Authors: Zhu Yong , Ding Ling , Xia Liang , He Wenhua , Xiong Huifang , Liu Pi , Shu Xu , Liu Zhijian , Zhu Yin , Chen Youxiang , Lv Nonghua
Published: March 2021

BackgroundAlthough metal stents have been widely used in the endoscopic step-up approach for necrotizing pancreatitis (NP), the exact timing of transmural stent removal has not been well studied. Here, we report a novel strategy for stent removal and compare it with the conventional strategy.MethodsThis retrospective, nested, case-control study analyzed all adult NP patients who underwent endoscopic transmural necrosectomy (ETN). Clinical outcomes were compared between case group (a novel strategy in which the stents were removed during the last necrosectomy when the ETN endpoint was achieved) and 1:1 necrosis extent matched control group (the conventional strategy in which the stents were removed after necrosectomy when clinical symptoms were relieved and collection was nearly completely resolved, as confirmed by imaging).ResultsBaseline characteristics was comparable between case group (n=37) and control group (n=37). Three (8.1%) patients in case group and one (2.7%) patient in control group needed additional ETN after stent removal (P >0.05). Nearly all patients (case: 97.3%; control: 94.6%) achieved clinical success. There was no difference in disease-related (case: 40.5%; control: 37.8%) or stent-related (case: 27.0%; control: 16.2%) complications. Case group had a trend of shorter length of stent placement (median: 20.0 days vs. 29.5 days, P >0.05).ConclusionsCompared to the convention strategy, the novel strategy of transmural stent removal during last necrosectomy avoids one endoscopy procedure and is feasible, with no need for additional ETN after stent removal, similar improvement in clinical outcomes, and no increase in complications. Prospective multicenter studies regarding the timing of transmural stent removal are needed to further validate our conclusions.
c-Abl Tyrosine Kinase Mediated Neuronal Apoptosis in Subarachnoid Hemorrhage by Modulating the LRP-1-Dependent Akt/GSK3β Survival Pathway
Authors: yan cong , yu hong wei , liu yao , wu pei , wang chun lei , zhao hong bo , yang kong bin , shao qi , zhong ying qiang , zhao wen yang , li jing wei , liu nan , di jing long , li chen , bao lu hao , gao cheng
Published: March 2021

Accumulating evidence suggests that neuronal apoptosis plays a critical role in early brain injury (EBI) after subarachnoid hemorrhage (SAH), and the inhibition of apoptosis can induce neuroprotective effects in SAH animal models. c-Abl has been reported to promote neuronal apoptosis in Alzheimer's disease and cerebral ischemia, but its role in SAH had not been illuminated until now. In the present study, the effect of c-Abl on neuronal apoptosis induced by SAH was investigated. c-Abl protein levels and neuronal apoptosis were markedly increased 24 h after SAH, and the inhibition of endogenous c-Abl reduced neuronal apoptosis and mortality and ameliorated neurological deficits. Furthermore, c-Abl inhibition decreased the expression of cleaved caspase-3 (CC-3) after SAH. These results demonstrate the proapoptotic effect of c-Abl in EBI after SAH. Additionally, c-Abl inhibition further enhanced the SAH-induced phosphorylation of Akt and glycogen synthase kinase (GSK)3β. LY294002 abrogated the beneficial effects of targeting c-Abl and exacerbated neuronal apoptosis after SAH. SAH decreased LRP-1 levels and downregulated LRP-1 by RAP and LRP-1 small interfering RNA (siRNA) induced a dramatic decrease in Akt/GSK3β activation in the presence of c-Abl siRNA. This is the first report showing that the c-Abl tyrosine kinase may play a key role in SAH-induced neuronal apoptosis by regulating the LRP-1-dependent Akt/GSK3β survival pathway. Thus, c-Abl has the potential to be a novel target for EBI therapy after SAH.
Buffering Effect of in-patient Psychiatric Care on the Link Between Fear of Covid-19 and Mental Health Consequences
Authors: Bloch Yuval , Shemesh Sharon , Grossman-Giron Ariella , Maoz Hagai , Cohenmehr Erica , Hertzberg Libi , Nitzan Uri , Tsur-Bitan Dana
Published: March 2021

Background& AimesPsychiatric admissions during the covid-19 pandemic were limited ignoring their possible benefit. The study focused on assessing the effect of the fear of covid on the mental health and well-being of in-patients as opposed to outpatients MethodsDuring the first lockdown, forty-four in-patients and day care patients (in-patient group) and 74 outpatients (out-patient group) were recruited after an informed consent procedure. Fear from the infection was assed using the Fear of COVID-19 (FCV-19S), severity of mental health symptomatology was evaluated with the outcome questionnaire-45 (OQ-45)., wellbeing was assessed with the Psychological well-being scale (PWB).OutcomesThere was no difference between the in-patient group and out-patient group in their fear of COVID-19 levels. FCV-19 predicted changes in the outpatient OQ total score (B=2.21, p<0.001), OQ interpersonal relation subscale (B=0.34, p=0.01), PWB total score (B=-0.05, p<0.001), PWB environmental mastery subscale (B=-.07, p<0.001) and PWB positive relation subscale (B=-0.05, p<0.001), but not in the inpatient group.ConclusionsMental health and well-being of the out-patient group that had less therapeutic contact –unlike the in-patient group-correlated with the fear of covid. Supporting the hypothesis that intensive psychiatric therapy had a protective effect from the mental health consequences of “fear of covid”.
RUSLE Model Based Annual Soil Loss Quantification for Soil Erosion Protection in Fincha Catchment, Abay River Basin, Ethiopia.
Authors: Tamiru Habtamu , Wagari Meseret
Published: March 2021

Background: The quantity of soil loss as a result of soil erosion is dramatically increasing in catchment where land resources management is very weak. The annual dramatic increment of the depletion of very important soil nutrients exposes the residents of this catchment to high expenses of money to use artificial fertilizers to increase the yield. This paper was conducted in Fincha Catchment where the soil is highly vulnerable to erosion, however, where such studies are not undertaken. This study uses Fincha catchment in Abay river basin as the study area to quantify the annual soil loss, where such studies are not undertaken, by implementing Revised Universal Soil Loss Equation (RUSLE) model developed in ArcGIS version 10.4. Results: Digital Elevation Model (12.5 x 12.5), LANDSAT 8 of Operational Land Imager (OLI) and Thermal Infrared Sensor (TIRS), Annual Rainfall of 10 stations (2010-2019) and soil maps of the catchment were used as input parameters to generate the significant factors. Rainfall erosivity factor (R), soil erodibility factor (K), cover and management factor (C), slope length and steepness factor (LS) and support practice factor (P) were used as soil loss quantification significant factors. It was found that the quantified average annual soil loss ranges from 0.0 to 76.5 t ha-1 yr-1 was obtained in the catchment. The area coverage of soil erosion severity with 55%, 35% and 10% as low to moderate, high and very high respectively were identified. Conclusion: Finally, it was concluded that having information about the spatial variability of soil loss severity map generated in the RUSLE model has a paramount role to alert land resources managers and all stakeholders in controlling the effects via the implementation of both structural and non-structural mitigations. The results of the RUSLE model can also be further considered along with the catchment for practical soil loss quantification that can help for protection practices.
Rehabilitation interventions to support return to work for women with breast cancer: a systematic review and meta-analysis
Authors: Algeo Naomi , Bennett Kathleen , Connolly Deirdre
Published: March 2021

Background
Research recommends the development and evaluation of interventions to support women with breast cancer in returning to, or managing, work. Despite this, there has historically been a paucity of rehabilitation interventions to support women with breast cancer to maintain or return to their work role.
The aim of this systematic review was
to examine key characteristics of rehabilitation interventions, and
their effectiveness on work outcomes for women with breast cancer,
compared to usual care.
Methods

A systematic review was conducted of
controlled studies of rehabilitation interventions with work
outcomes for women with breast cancer.
Six databases were systematically searched: EMBASE, Web of Science, MEDLINE (OVID), CINAHL, PsycINFO, and the Cochrane Central Register of Controlled Trials (CENTRAL). Results are presented either as pooled odds ratio (OR) or pooled effect size (hedges g) between groups, with 95% confidence intervals (CI). Narrative synthesis was conducted on intervention outcomes not suitable for meta-analysis.
Results
Five thousand, five hundred and thirty-five studies were identified. Nine out of 28 abstracts met inclusion criteria. Heterogeneity of interventions and outcomes precluded meta-analysis for most outcomes. Of the interventions included in meta-analysis, no significant differences compared to usual care were found for sick leave (2 studies (12 months); OR 1.11 (95% CI: 0.66 to 1.87), number of sick days taken (2 studies (six months); difference in effect: -0.08, (95% CI: -0.48 to 0.38) or working hours (2 studies (12 months); 0.19, (95% CI: -0.20 to 0.64). Only one study, with a multidisciplinary intervention, showed a significant difference for work outcomes when compared to usual care. Work-specific content featured in three interventions only, none of which provided conclusive evidence for improvement in work outcomes. Enhanced physical and psychological sequalae, and quality of life was observed in some studies.
Conclusion
There remains a lack of effective and methodologically rigorous rehabilitation intervention studies for breast cancer survivors. The development and evaluation of effective rehabilitation interventions to support return to work is warranted.
Genome Assessment of Antibiotic Resistance Genes in Probiotic Bacteria and a Literature Review
Authors: Fatahi-Bafghi Mehdi , Naseri Sara , Alizehi Ali
Published: March 2021

Having various clinical applications, probiotic bacteria are currently used in the diet. There are reports of antibiotic resistance genes (ARGs) in these bacteria that can be transferred to other microflora and pathogenic bacteria. The aim of the study is to examine whole-genome sequence analysis in bacteria with probiotic properties. Moreover, this study follows existing issues about the importance and presence of ARGs in these bacteria the dangers of which may affect human health in the years to come. In the present study, 126 complete probiotic bacterial genomes were collected and analysed for ARGs. The results of the study shows there are various antibiotic resistant genes of in these bacteria some of which can be transmitted to other bacteria. We propose microorganisms be applied as a probiotic element in various types of products, antibiogram be conducted for a large number of antibiotics and analysis of complete genome sequence for ARGs prediction.
Multiplex PCR assay for the identification of eight Anopheles species belonging to the Hyrcanus, Barbirostris, and Lindesayi groups (Diptera: Culicidae)
Authors: Bang WooJun , Kim Heung-Chul , Ryu Jihun , Lee Hyeon Seung , Lee So Youn , Kim Myung Soon , Chong Sung Tae , Klein Terry A. , Choi Kwang Shik
Published: March 2021

Background

Genus Anopheles mosquitoes are the primary vectors of human malaria, which is a serious threat to public health worldwide. To reduce the spread of malaria and identify the malaria infection rates in mosquitoes, accurate species identification is needed. Malaria reemerged in 1993 in the Republic of Korea (ROK), with numbers peaking in 2004 before decreasing to current levels. Eight Anopheles species (Anopheles sinensis, An. pullus, An. belenrae, An. lesteri, An. kleini, An. sineroides, An. koreicus, and An. lindesayi) are distributed throughout Korea. Members of the Anopheles Hyrcanus Group currently cannot be identified morphologically. The other species of Anopheles can be identified morphologically, except when specimens are damaged in traps. The purpose of this study was to develop a rapid and accurate method for simultaneous molecular identification of the eight Anopheles species present in the ROK.
Methods

Anopheles spp. used in this study were collected near/in the demilitarized zone in the ROK, where most malaria cases are reported. DNA from 165 of the Anopheles specimens was used to develop a multiplex PCR assay. The internal transcribed spacer 2 (ITS2) region of each species was sequenced and analyzed for molecular identification.
Results

DNA from a total of 165 Anopheles specimens was identified to species using a multiplex diagnostic system. These included: 20 An. sinensis, 21 An. koreicus, 17 An. lindesayi, 25 An. kleini, 11 An. lesteri, 22 An. sineroides, 23 An. belenrae, and 26 An. pullus. Molecular weights determined by electrophoresis were 1112 bp for An. sinensis, 925 bp for An. koreicus, 650 bp for An. lindesayi, 527 bp for An. kleini, 436 bp for An. lesteri, 315 bp for An. sineroides, 260 bp for An. belenrae, and 157 bp for An. pullus.
Conclusion

A multiplex PCR assay was developed to identify Anopheles spp. distributed in the ROK. This method can be used to accurately identify Anopheles species that are difficult to identify morphologically to determine species distributions and malaria infection rates.
 The Early Maternal Environment Shapes the Parental Response to Offspring UV Ornamentation
Authors: García-Campa Jorge , Müller Wendt , Hernández-Correas Ester , Morales Judith
Published: March 2021

Parents allocate resources to offspring to increase their survival and to maximize their own fitness, while this investment implies costs to their condition and future reproduction. Parents are hence expected to optimally allocate their resources. They should invest equally in all their offspring under good conditions, but when parental capacity is limited, parents should invest in the offspring with the highest probability of survival. Such parental favouritism is facilitated by the fact that offspring have evolved condition-dependent traits to signal their quality to parents. In this study we explore whether the parental response to an offspring quality signal depends on the intrinsic capacity of the parents, here the female. We first manipulated the intrinsic capacity of blue tit (Cyanistes caeruleus) females through lutein-supplementation during egg laying, and we subsequently blocked the UV/yellow reflectance of breast feathers on half of the nestlings in each brood. However, we did not find evidence that the female intrinsic capacity shaped parental favouritism for offspring UV/yellow colouration, as there were no differences in parental feeding or sibling competition. However, we found that males were more responsive than females to nestling UV/yellow when rearing capacity was high, as indicated by the prey-testings (when a parent places a prey item into a nestling’s gape but removes it again). Furthermore, when considering a more integrative measure, offspring growth, we did find the expected interaction effect. In control nests, UV-blocked nestlings gained less body mass than their non-UV-blocked siblings, whereas in lutein-supplemented nests UV-blocked nestlings gained more mass than their siblings. Overall, our results emphasize the female’s environment at an early reproduction stage shaped the role of offspring UV/yellow during family interactions illustrating plasticity in parental feeding rules.
The Chloroplast Genome Evolution of Venus Slipper (Paphiopedilum): IR Expansion, SSC Contraction, and Highly Rearranged SSC Regions
Authors: Guo Yan-Yan , Yang Jia-Xing , Zhang Guo-Qiang , Liu Zhong-Jian
Published: March 2021

Background: Paphiopedilum is the largest genus of slipper orchids. Previous studies showed that the phylogenetic relationships of this genus are not well resolved, and sparse taxon sampling documented inverted repeat (IR) expansion and small single copy (SSC) contraction of the chloroplast genomes of Paphiopedilum. Here, we sequenced, assembled, and annotated 77 plastomes of Paphiopedilum species. The phylogeny based on the plastome resolved the relationships of the genus except for the phylogenetic position of two unstable species. We used phylogenetic and comparative genomic approaches to elucidate the plastome evolution of Paphiopedilum.Results: The plastomes of Paphiopedilum have conserved genome structure and gene content except in the SSC region. The large single copy/inverted repeat (LSC/IR) boundaries are relatively stable, while the boundaries of inverted repeat/small single copy (IR/SSC) boundaries varied among species. Corresponding to the IR/SSC boundary shifts, the chloroplast genomes of the genus experienced IR expansion and SSC contraction. The IR region incorporated one to six genes of the SSC region. Unexpectedly, great variation in the size, gene order, and gene content of the SSC regions was found, especially in the subg. Parvisepalum. Furthermore, Paphiopedilum provides evidence for the ongoing degradation of the ndh genes in the photoautotrophic plants. The estimated substitution rates of the protein coding genes show accelerated rates of evolution in clpP, psbH, and psbZ. Genes transferred to the IR region due to the boundary shift also have higher substitution rates.Conclusions: We found IR expansion and SSC contraction in the chloroplast genomes of Paphiopedilum with dense sampling, and the genus shows variation in the size, gene order, and gene content of the SSC region. This genus provides an ideal system to investigate the dynamics of plastome evolution.
Experimental Investigation of a Pilot-Scale Concerning Ex-Situ Bioremediation of Petroleum Hydrocarbons Contaminated Soils
Authors: Micle Valer , Sur Ioana
Published: March 2021

The soil samples were taken from the site of a former oil products depot from an industrial area (Romania). The soil samples taken were analyzed from a physical and chemical point of view: texture, ph, soil micronutrient content, metals concentration and petroleum hydrocarbon concentration (PHCs). The soil contaminated with TPH (4280 mgkg-1) was disposed in the form of a pile (LxWxH:3000x1400x500 mm). Experiments a pilot-scale were conducted over 12 weeks at constant pH (7.5–8), temperature (22–32oC), nutrient contents C/N/P ratio 100:10:1, soil aeration time (8 hour/day) and moisture (30%). Samples were taken every two weeks for the monitoring of the TPH and the microorganisms content. During experiment every two weeks were added microoganisms (Pseudomonas and Bacillus). Results of the analyzes regarding the concentration of PHCs were revelead a linear decrease of the concentration of PHCs after only two weeks of treatment. This decrease in concentration was also achieved in the following weeks. Following the analysis performed on the model at the pilot scale regarding the depollution process, it can be concluded that a soil contaminated with petroleum hydrocarbons can be efficiently depolluted by performing an aeration of 8 h/day, adding microorganisms Pseudomonas and Bacillus to ensure the conditions for increasing in the total number of germs (colony forming units–CFU) from 151x105 to 213x107 CFU/gram of soil, after 12 weeks of soil treatment - the depollution efficiency achieved is 83%.
Optimizing Telemedical Care in Neurological Outpatients by Characterizing the Patients’ Needs in Physician-Patient Relationship - Content Analysis of Guideline-Based Interviews
Authors: Hamann Till , Lemke Stella , Kropp Peter , Rimmele Florian , Jürgens Tim P. , Frielitz Fabian
Published: March 2021

BackgroundThe use of new concepts in patient care, such as video-consultation, reminder systems and online-evaluation portals is becoming increasingly important in the physician-patient relation and in the care of outpatients. This study examines the acceptance of these approaches in a neurological setting and characterizes the patients’ preferences.MethodsWe analyzed 16 guideline-based qualitative interviews with neurological patients using qualitative content analysis (inductive category formation). ResultsThe patients commented on benefits and challenges of integrating new concepts of medical care. They identified advantages of telemedical care, including time savings for both the patient and the physician, the prospect of more intensive care and the possibility of quick response in case of urgent needs. Several challenges were reported, such as the limitations for patients with chronic or complex diseases and limited options for diagnostic procedures (such as physical examination)For individual neurological patient’s needs, telemedical and telecommunication structures could be discussed which support the respective requirements of the patients such as answering questions while having a recall, avoiding of the journey and time-savings. Also, they are rejecting evaluation portals and are skeptical about telecare in the treatment of neurological diseases.Discussion/ConclusionsThe neurological patients can be characterized as individuals with different ideas of an optimal physician-patient relationship. A successful integration of new concepts in medical care depends on fulfilling the individual patient’s needs. Regardless of the preferred form of the physician-patient relationship, there are specific instruments that can intensify the relationship. These individual needs of the patients must be inquired about and implemented.
“She Knows Me Best”: A Qualitative Study of Patient and Caregiver Views on the Role of the Primary Care Physician Follow-Up Post-Discharge
Authors: Griffiths Sarah , Stephen Gaibrie , Kiran Tara , Okrainec Karen
Published: March 2021

BackgroundPatients with chronic obstructive pulmonary disease (COPD) and congestive heart failure (CHF) are at high-risk of readmission after hospital discharge. There is conflicting evidence however on whether timely follow-up with a primary care provider reduces that risk. The objective of this study is to understand the perspectives of patients with COPD and CHF, and their caregivers, on the role of primary care provider follow-up after hospital discharge.MethodsA qualitative study design with semi-structured interviews was conducted among patients or their family caregivers admitted with COPD or CHF who were enrolled in a multicenter mixed-methods study at three acute care hospitals in Ontario, Canada. Participants were interviewed between December 2017 to January 2019, the majority discharged from hospital at least 30 days prior to their interview. Interviews were analyzed independently by three authors using a deductive directed content analysis, with the fourth author cross-comparing themes.ResultsInterviews with 16 participants (eight patients and eight caregivers) revealed four main themes. First, participants valued visiting their primary care provider after discharge to build upon their longitudinal relationship. Second, primary care providers played a key role in coordinating care. Third, there were mixed views on the ideal time for follow-up, with many participants expressing a desire to delay follow-up to stabilize following their acute hospitalization. Fourth, the link between the post-discharge visit and preventing hospital readmissions was unclear to participants, who often self-triaged based on their symptoms when deciding on the need for emergency care.ConclusionsPatients and caregivers valued in-person follow-up with their primary care provider following discharge from hospital because of the trust established through pre-existing longitudinal relationships. Our results suggest policy makers should focus on improving rates of primary care provider attachment and systems supporting informational continuity.
Cervical Cancer Screening Uptake and Determinant Factors Among Women in Ambo Town, Western Oromia, Ethiopia: Community based Cross Sectional Study
Authors: Natae Shewaye Fituma , Nigatu Digafe Tsegaye , Negawo Mulu Kitaba , Mengesha Wakeshe Willi
Published: March 2021

Background: Cervical cancer is one of the principal causes of cancer death among women worldwide. It is the second most common cancer and the leading cause of cancer-related death in Ethiopian women; about 77.6% of women died of 6,294 new cases reported in 2019. Early screening for cervical cancer has substantial advantage to reduce the incidence, morbidity, and mortality attributed to cervical cancer. So far, there are limited evidence on the level of cervical cancer screening uptake and its determinant in low- and middle-income countries including Ethiopia. Consequently, the current study aimed to assess the level of cervical cancer screening uptake and its determinant among women of Ambo town, western Oromia, Ethiopia.Methods: A community-based cross-sectional study was conducted among 422 women aged 20-65years. Systematic random sampling was employed to recruit the eligible women from randomly selected sub-districts found in Ambo town. Interviewer-administered questionnaire was used to collect the data. Epi info was used for data entry and management from which it was exported to SPSS version 25 for detailed analysis. Variables in binary logistic regression with a p-value <0.25 were candidate for multivariable logistic regression. Estimates were presented using odds ratios (ORs) with 95% CI. Statistical Significance was declared at p value<0.05.Results: In the present study 392 women were participated giving a response rate of 93%. Overall, 63% of the respondents were known the availability of cervical cancer screening service. Only 8.7% (34) of the study participants were received cervical cancer screening in their lifetime. Being in the age group of 30-39 years (AOR=3.2; 95% CI [1.22, 8.36]) and 40-49years (AOR=4.8; 95% CI [1.42, 16.41]), having cervical cancer related discussion with health care provider (AOR=3.5; 95% CI [1.17, 10.7]), and knowing availability cervical cancer screening service (AOR=2.8; 95% CI [1.03, 7.87]) were significantly associated with uptake of cervical cancer screening. Conclusion: In this study, cervical cancer screening uptake is very low. The study also highlighted important factors that affect uptake of cervical cancer screening service. Therefore, this study result call urgent interventions by all stockholders to increase cervical cancer service uptake through continues promotions and by securing its availability and accessibility for all eligible women at all levels.
Pediatric Soft Tissue Injuries During the COVID-19 Lockdown
Authors: Olshinka Asaf , Shoufani Rami , Shachar Tal , Lvovsky Alex , Kalish Eyal , Ad-El Dean D. , Yaacobi Dafna (Shilo)
Published: March 2021

The authors have requested that this preprint be removed from Research Square.
A New Approach to Produce IgG4-like Bispecific Antibodies
Authors: Zhao Caizhi , Zhang Wei , Gong Guihua , Xie Liping , Wang Ming-Wei , Hu Youjia
Published: March 2021

While achieving rapid developments in recent years, bispecific antibodies are still difficult to design and manufacture, due to mismatch of both heavy and light chains. Here we report a novel technology to make correct bispecific molecules. The knob-into-hole method was used to pair two distinct heavy chains as a heterodimer. IgG4 S228P CH1-CL interface was then partially replaced by T-cell receptor α/β constant domain to increase the efficiency of cognate heavy and light chain pairing. Following expression and purification, the bispecific antibody interface exchange was confirmed by DNA sequencing and LC-MS/MS. To ensure its validity, we combined a monovalent bispecific antibody against PD-1 (sequence from Pembrolizumab) and LAG3 (sequence from Relatlimab). The results showed that the molecule could be assembled correctly at a ratio of 95% in cells. In vitro functional assay demonstrated that the purified bispecific antibody exhibits an enhanced agonist activity compared to that of the parental antibodies. Low immunogenicity was predicted by an open-access software and ADA test.
COVID-19 in Conflict Border Regions: A Case of South Kordofan, Sudan
Authors: Sserwanja Quraish , Adam Mohammed Bashir , Kawuki Joseph , Olal Emmanuel
Published: March 2021

The novel coronavirus disease (COVID-19) was first reported in Sudan on 13 March 2020. Since then, Sudan has experienced one of the highest COVID-19 spread and fatalities in Africa. As per 12 December 2020, Sudan had a total of 21,591 cases, 1,355 cumulative deaths at a case fatality rate (CFR) of 6.28%. South Kordofan State has 17.4% COVID-19 case fatality rate, the fifth highest rate among the 18 States. The State is home to over 200,000 poor and displaced people from years of destructive civil unrests. To date, several localities such as the Nubba mountains region remain under rebel control and are not accessible. South Kordofan State Ministry of Health in collaboration with the federal government and non-governmental organizations set up four isolation centres with 40 total bed capacity, but with only two mechanical ventilators and no testing centre. There is still need for further multi-sectoral coalition and equitable allocation of resources to strengthen the health systems of rural and conflict affected regions. This article aims at providing insight into the current State of COVID-19 in South Kordofan amidst the second wave to address the dearth of COVID-19 information in rural and conflict affected regions.