bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Root Color and Tissue on Phytochemical Contents and Antioxidant Activities in Carrot Genotypes
Authors: Bhandari Shiva Ram , Choi Chang Sun , Rhee Juhee , Shin Yu Kyeong , Song Jae Woo , Kim Seong-Hoon , Kang Solly , Lee Jun Gu
Published: December 2022

This study monitored changes in major carotenoids (lutein, ⍺-carotene, and β-carotene), free sugars (fructose, glucose, and sucrose), ascorbic acid, vitamin E, phytosterols (campesterol, stigmasterol, and β-sitosterol), fatty acid composition, total phenol content (TPC), total flavonoid content (TFC), total anthocyanin content, and antioxidant activities (AA); ferric-reducing antioxidant power (FRAP) and 2,2′-azino-bis (3-ethylbenzothiazoline-6sulfonic acid) [ABTS] assays, in the inner and outer root tissues of nine carrot genotypes with orange, white, and purple roots. The results showed a differential accumulation of bioactive compounds and antioxidant activities depending on root tissue and color. Carotenoids, free sugars, and total phytosterol contents were higher in genotypes with orange roots than in other genotypes. Ascorbic acid, TPC, TFC, total anthocyanin, and AA were highest in purple-colored carrots while vitamin E content was higher in white/purple carrots. Root color was highly related to the accumulation of individual carotenoids, vitamin E isomers, and total anthocyanin content most prominently among the analyzed bioactive compounds and AA. Free sugar and carotenoid contents were relatively higher in outer tissues than in inner tissues. Furthermore, ascorbic acid, TPC, TFC, and AA were statistically higher or similar in outer tissues when compared to inner tissues in all genotypes. In contrast, trends in vitamin E and phytosterol content were inconsistent between the inner and outer tissues, depending on the genotype. Although fatty acid composition was affected by both root color and tissue, the results were not significant. Thus, the phytochemical profile and content were highly dependent on root color and tissue in carrot genotypes. This may be useful in the food processing and pharmaceutical industries for the extraction of targeted bioactive compounds.
Some Compound Fractional Poisson Processes
Authors: Khandakar Mostafizar , Kataria Kuldeep Kumar
Published: December 2022

In this paper, we introduce and study fractional versions of the Bell–Touchard process, the Poisson-logarithmic process and the generalized Pólya–Aeppli process. The state probabilities of these compound fractional Poisson processes solve a system of fractional differential equations that involves the Caputo fractional derivative of order 0<β<1. It is shown that these processes are limiting cases of a recently introduced process, namely, the generalized counting process. We obtain the mean, variance, covariance, long-range dependence property, etc., for these processes. Further, we obtain several equivalent forms of the one-dimensional distribution of fractional versions of these processes.
Ferroptosis: A Novel Type of Cell Death in Male Reproduction
Authors: Su Yanjing , Liu Zelan , Xie Keyu , Ren Yingxin , Li Chunyun , Chen Wei
Published: December 2022

Ferroptosis, an iron-dependent type of regulated cell death, is triggered by the accumulation of lethal lipid peroxides. Due to its potential in exploring disease progression and highly targeted therapies, it is still a widely discussed topic nowadays. In recent studies, it was found that ferroptosis was induced when testicular tissue was exposed to some high-risk factors, such as cadmium (Cd), busulfan, and smoking accompanied by a variety of reproductive damage characteristics, including changes in the specific morphology and ferroptosis-related features. In this literature-based review, we summarize the related mechanisms of ferroptosis and elaborate upon its relationship network in the male reproductive system in terms of three significant events: the abnormal iron metabolism, dysregulation of the Cyst(e)ine/GSH/GPX4 axis, and lipid peroxidation. It is meaningful to deeply explore the relationship between ferroptosis and the male reproductive system, which may provide suggestions regarding pristine therapeutic targets and novel drugs.
Melatonin and MitoEbselen-2 Are Radioprotective Agents to Mitochondria
Authors: Shimura Tsutomu , Shiga Rina , Sasatani Megumi , Kamiya Kenji , Ushiyama Akira
Published: December 2022

Mitochondria are responsible for controlling cell death during the early stages of radiation exposure, but their perturbations are associated with late effects of radiation-related carcinogenesis. Therefore, it is important to protect mitochondria to mitigate the harmful effects of radiation throughout life. The glutathione peroxidase (GPx) enzyme is essential for the maintenance of mitochondrial-derived reactive oxygen species (ROS) levels. However, radiation inactivates the GPx, resulting in metabolic oxidative stress and prolonged cell injury in irradiated normal human fibroblasts. Here, we used the GPx activator N-acetyl-5-methoxy-tryptamine (melatonin) and a mitochondria-targeted mimic of GPx MitoEbselen-2 to stimulate the GPx. A commercial GPx activity assay kit was used to measure the GPx activity. ROS levels were determined by using some ROS indicators. Protein expression associated with the response of mitochondria to radiation was assessed using immunostaining. Concurrent pre-administration or post-administration of melatonin or MitoEbselen-2 with radiation maintained GPx activity and ROS levels and suppressed mitochondrial radiation responses associated with cellular damage and radiation-related carcinogenesis. In conclusion, melatonin and MitoEbselen-2 prevented radiation-induced mitochondrial injury and metabolic oxidative stress by targeting mitochondria. These drugs have the potential to protect against acute radiation injury and late effects of carcinogenesis in a variety of radiation scenarios assuming pre-administration or post-administration.
Whole-Exome Sequencing of Pakistani Consanguineous Families Identified Pathogenic Variants in Genes of Intellectual Disability
Authors: Asif Maria , Anayat Maryam , Tariq Faiza , Noureen Tanzeela , Din Ghulam Naseer Ud , Becker Christian , Becker Kerstin , Thiele Holger , Makhdoom Ehtisham ul Haq , Shaiq Pakeeza Arzoo , Baig Shahid M. , Nürnberg Peter , Hussain Muhammad Sajid , Raja Ghazala Kaukab , Abdullah Uzma
Published: December 2022

Intellectual disability (ID) is a condition of significant limitation of cognitive functioning and adaptive behavior, with 50% of etiology attributed to genetic predisposition. We recruited two consanguineous Pakistani families manifesting severe ID and developmental delay. The probands were subjected to whole exome sequencing (WES) and variants were further prioritized based on population frequency, predicted pathogenicity and functional relevance. The WES data analysis identified homozygous pathogenic variants in genes MBOAT7 and TRAPPC9. The pathogenicity of the variants was supported by co-segregation analysis and in silico tool. The findings of this study expand mutation spectrum and provide additional evidence to the role of MBOAT7 and TRAPPC9 in causation of ID.
Genetic Diversity of Cytochrome P450s CYP6M2 and CYP6P4 Associated with Pyrethroid Resistance in the Major Malaria Vectors Anopheles coluzzii and Anopheles gambiae from Yaoundé, Cameroon
Authors: Fotso-Toguem Yvan , Tene-Fossog Billy , Mugenzi Leon M. J. , Wondji Murielle J. , Njiokou Flobert , Ranson Hilary , Wondji Charles S.
Published: December 2022

Assessing the genetic diversity of metabolic resistance genes, such as cytochrome P450s, helps to understand the dynamics and evolution of resistance in the field. Here, we analyzed the polymorphisms of CYP6M2 and CYP6P4, associated with pyrethroid resistance in Anopheles coluzzii and Anopheles gambiae, to detect potential resistance markers. Field-caught resistant mosquitos and susceptible lab strains were crossed, and F4 was exposed to permethrin for 15 min and 90 min to discriminate highly susceptible (HS) and highly resistant (HR) mosquitos, respectively. Significant permethrin mortality reduction was observed after pre-exposure to PBO, suggesting the gene involvement of P450s. qPCR analysis revealed significant overexpression of CYP6M2 (FC = 19.57 [95% CI 13.96–25.18] for An. coluzzii; 10.16 [7.86–12.46] for An. gambiae) and CYP6P4 (FC = 6.73 [6.15–7.30] An. coluzzii; 23.62 [26.48–20.76] An. gambiae). Full-gene and ≈1 kb upstream were sequenced. For CYP6M2, the upstream region shows low diversity in HR and HS (overall Hd = 0.49, π = 0.018), whereas the full-gene shows allelic-variation but without evidence of ongoing selection. CYP6P4 upstream region showed a lower diversity in HR (Hd = 0.48) than HS (Hd = 0.86) of An. gambiae. These results highlighted that CYP6P4-associated resistance is potentially driven by modification in upstream region. However, further work is needed to determine the real causative variants that will help design rapid detection tools.
Characterization of DNA Polymerase Genes in Amazonian Amerindian Populations
Authors: Cohen-Paes Amanda , de Alcântara Angélica Leite , de Souza Menezes Elisa , Moreira Fabiano Cordeiro , Fernandes Marianne Rodrigues , Guerreiro João Farias , Ribeiro-Dos-Santos Ândrea , Dos Santos Sidney Emanuel Batista , Santos Ney Pereira Carneiro dos
Published: December 2022

Due to their continuing geographic isolation, the Amerindian populations of the Brazilian Amazon present a different genetic profile when compared to other continental populations. Few studies have investigated genetic variants present in these populations, especially in the context of next-generation sequencing. Knowledge of the molecular profile of a population is one of the bases for inferences about human evolutionary history, in addition, it has the ability to assist in the validation of molecular biomarkers of susceptibility to complex and rare diseases, and in the improvement of specific precision medicine protocols applied to these populations and to populations with high Amerindian ancestry, such as Brazilians. DNA polymerases play essential roles in DNA replication, repair, recombination, or damage repair, and their influence on various clinical phenotypes has been demonstrated in the specialized literature. Thus, the aim of this study is to characterize the molecular profile of POLA1, POLE, POLG, POLQ, and REV3L genes in Amerindian populations from the Brazilian Amazon, comparing these findings with genomic data from five continental populations described in the gnomAD database, and with data from the Brazilian population described in ABraOM. We performed the whole exome sequencing (WES) of 63 Indigenous individuals. Our study described for the first time the allele frequency of 45 variants already described in the other continental populations, but never before described in the investigated Amerindian populations. Our results also describe eight unique variants of the investigated Amerindians populations, with predictions of moderate, modifier and high clinical impact. Our findings demonstrate the unique genetic profile of the Indigenous population of the Brazilian Amazon, reinforcing the need for further studies on these populations, and may contribute to the creation of public policies that optimize not only the quality of life of this population, but also of the Brazilian population.
Genome-Wide Identification and Analysis of the GRAS Transcription Factor Gene Family in Theobroma cacao
Authors: Hou Sijia , Zhang Qianqian , Chen Jing , Meng Jianqiao , Wang Cong , Du Junhong , Guo Yunqian
Published: December 2022

GRAS genes exist widely and play vital roles in various physiological processes in plants. In this study, to identify Theobroma cacao (T. cacao) GRAS genes involved in environmental stress and phytohormones, we conducted a genome-wide analysis of the GRAS gene family in T. cacao. A total of 46 GRAS genes of T. cacao were identified. Chromosomal distribution analysis showed that all the TcGRAS genes were evenly distributed on ten chromosomes. Phylogenetic relationships revealed that GRAS proteins could be divided into twelve subfamilies (HAM: 6, LISCL: 10, LAS: 1, SCL4/7: 1, SCR: 4, DLT: 1, SCL3: 3, DELLA: 4, SHR: 5, PAT1: 6, UN1: 1, UN2: 4). Of the T. cacao GRAS genes, all contained the GRAS domain or GRAS superfamily domain. Subcellular localization analysis predicted that TcGRAS proteins were located in the nucleus, chloroplast, and endomembrane system. Gene duplication analysis showed that there were two pairs of tandem repeats and six pairs of fragment duplications, which may account for the rapid expansion in T. cacao. In addition, we also predicted the physicochemical properties and cis-acting elements. The analysis of GO annotation predicted that the TcGRAS genes were involved in many biological processes. This study highlights the evolution, diversity, and characterization of the GRAS genes in T. cacao and provides the first comprehensive analysis of this gene family in the cacao genome.
Improved Outcomes in Patients with 22q11.2 Deletion Syndrome and Diagnosis of Interrupted Aortic Arch Prior to Birth Hospital Discharge, a Retrospective Study
Authors: Ron Hayley A. , Crowley Terrence Blaine , Liu Yichuan , Unolt Marta , Schindewolf Erica , Moldenhauer Julie , Rychik Jack , Goldmuntz Elizabeth , Emanuel Beverly S. , Ryba Douglas , Gaynor James William , Zackai Elaine H. , Hakonarson Hakon , McDonald-McGinn Donna M.
Published: December 2022

Interruption of the aortic arch (IAA) is a rare but life-threatening congenital heart defect if not corrected in the neonatal period. IAA type B is highly correlated with 22q11.2 deletion syndrome (22q11.2DS); approximately 50% of patients with IAA type B also have 22q11.2DS (Peyvandi et al.; Goldmuntz et al.). Early identification and repair of IAA can prevent severe morbidity and death. However, IAA is challenging to identify prenatally, or even in the neonatal period. In this study, we examined infants with IAA, diagnosed during pregnancy and prior to discharge (PPTD) from the birth hospital vs. those diagnosed following discharge (FD) from the newborn nursery. Our goals were to determine: (1) if early diagnosis improved outcomes; and (2) if patients with IAA and without 22q11.2DS had similar outcomes. In total, 135 patients with a diagnosis of 22q11.2DS and IAA were ascertained through the 22q and You Center at the Children’s Hospital of Philadelphia (CHOP). The examined outcomes included: timing of diagnosis; age at diagnosis (days); hospital length of stay (LOS); duration of intensive care unit (ICU) stay; mechanical ventilation (days); duration of inotrope administration (days); year of surgical intervention; birth hospital trauma level; and overall morbidity. These outcomes were then compared with 40 CHOP patients with IAA but without 22q11.2DS. The results revealed that the PPTD neonates had fewer days of intubation, inotrope administration, and hospital LOS when compared to the FD group. The outcomes between deleted and non-deleted individuals with IAA differed significantly, in terms of the LOS (40 vs. 39 days) and time in ICU (28 vs. 24 days), respectively. These results support the early detection of 22q11.2DS via prenatal screening/diagnostics/newborn screening, as IAA can evade routine prenatal ultrasound and postnatal pulse oximetry. However, as previously reported in patients with 22q11.2DS and congenital heart disease (CHD), patients with 22q11.2DS tend to fare poorer compared to non-deleted neonates with IAA.
Identification of Circular RNAs of Testis and Caput Epididymis and Prediction of Their Potential Functional Roles in Donkeys
Authors: Sun Yan , Wang Yonghui , Li Yuhua , Akhtar Faheem , Wang Changfa , Zhang Qin
Published: December 2022

Circular RNAs (circRNAs) are a class of noncoding RNAs with a covalently closed loop. Studies have demonstrated that circRNA can function as microRNA (miRNA) sponges or competing endogenous RNAs. Although circRNA has been explored in some species and tissues, the genetic basis of testis development and spermatogenesis in donkeys remain unknown. We performed RNA-seq to detect circRNA expression profiles of adult donkey testes. Length distribution and other characteristics were shown a total of 1971 circRNAs were differentially expressed and 12,648 and 6261 circRNAs were detected from the testis and caput epididymis, respectively. Among these circRNAs, 1472 circRNAs were downregulated and 499 circRNAs were upregulated in the testis. Moreover, KEGG pathway analyses and Gene Ontology were performed for host genes of circRNAs. A total of 39 upregulated circRNA host genes were annotated in spermatogenesis terms, including PIWIL2, CATSPERD, CATSPERB, SPATA6, and SYCP1. Other host genes were annotated in the focal adhesion, Rap1 signaling pathway. Downregulated expressed circRNA host genes participated in the TGF-β signaling pathway, GnRH signaling pathway, estrogen signaling pathway, and calcium signaling pathway. Our discoveries provide a solid foundation for identifying and characterizing critical circRNAs involved in testis development or spermatogenesis.
Lentivirus Susceptibility in Brazilian and US Sheep with TMEM154 Mutations
Authors: Rodrigues Camila Souza , de Faria Danielle Assis , Lacerda Thaísa Sant’Anna , Paiva Samuel Rezende , Caetano Alexandre Rodrigues , Blackburn Harvey , McManus Concepta
Published: December 2022

Small ruminant lentiviruses (SRLVs) affect sheep and goats worldwide. The major gene related to SRLV infections is the Transmembrane Protein Gene 154 (TMEM154). We estimated the haplotype frequencies of TMEM154 in the USA (USDA-ARS) and Brazil (Embrapa) Gene Banks by using two different SNP genotyping methodologies, FluidigmTM and KASPTM. We also genotyped the ZNF389_ss748775100 deletion variant in Brazilian flocks. A total of 1040 blood samples and 112 semen samples from 15 Brazilian breeds were genotyped with Fluidigm for the SNP ZNF389_ss748775100 and 12 TMEM154 SNPs. A total of 484 blood samples from the Santa Inês breed and 188 semen samples from 14 North American sheep breeds were genotyped with KASP for 6 TMEM154 SNPs. All the Brazilian samples had the “I/I” genotype for the ZNF389_ss748775100 mutation. There were 25 TMEM154 haplotypes distributed across the Brazilian breeds, and 4 haplotypes in the US breeds. Haplotypes associated with susceptibility were present in almost all breeds, which suggests that genetic testing can help to improve herd health and productivity by selecting non-susceptible animals as founders of the next generations. Fluidigm and KASP are reliable assays when compared with Beadchip arrays. Further studies are necessary to understand the unknown role of TMEM154 mutations, host–pathogen interaction and new genes associated with the clinical condition.
Genetic Diversity and New Sequence Types of Escherichia coli Coharboring β-Lactamases and PMQR Genes Isolated from Domestic Dogs in Central Panama
Authors: Núñez-Samudio Virginia , Pimentel-Peralta Gumercindo , De La Cruz Alexis , Landires Iván
Published: December 2022

Background: β-lactamase-producing Escherichia coli are a widely distributed source of antimicrobial resistance for animals and humans. Little is known about the susceptibility profile and genetic characteristics of E. coli strains isolated from domestic dogs in Latin America. Methods: We report on a cross-sectional study that evaluated E. coli strains isolated from fecal samples of domestic dogs in central Panama. The extended-spectrum β-lactamase (ESBL), AmpC genes, and plasmid-mediated quinolone resistance were investigated. Molecular typing using Pasteur’s multilocus sequence typing (MLST) was conducted. Results: A total of 40 E. coli isolates were obtained, of which 80% (32/40) were resistant to at least one of the antibiotics tested, while 20% (8/40) were sensitive to all antibiotics analyzed in this study (p < 0.001). Forty percent of the strains were resistant to three or more antibiotics. The most common resistance was to tetracycline (45%) and ampicillin (30%) while 2.5% showed an ESBL phenotype. Antibiotic resistance genes were detected for one β-lactamase (blaTEM-1) and two plasmid-mediated quinolone resistance (PMQR) enzymes (qnrS and qnrB). In addition, mutations in the chromosomal AmpC gene were observed at positions −35, −28, −18, −1, and +58. Fourteen different sequence types (STs) were identified; the most frequent were ST399 and ST425 (12% each). ST3 and ST88, which have been previously identified in human clinical isolates, were also evidenced. Three new STs were found for the first time: ST1015, ST1016 (carrier of the blaTEM-1 gene), and ST1017 (carrier of the blaTEM-1, qnrS, and qnrB genes). Conclusions: In the intestinal strains of E. coli isolated from domestic dogs, there was a high frequency of resistance to antibiotics. The presence of genes from plasmids and chromosomal mutations that conferred antibiotic resistance, the identification of isolates previously reported in humans, and the genetic diversity of STs (including three that were newly identified) confirmed the determinants of resistance to antibiotics in the domestic dogs from central Panama.
Genetic Diagnosis for 64 Patients with Inherited Retinal Disease
Authors: Lynn Jacob , Raney Austin , Britton Nathaniel , Ramoin Josh , Yang Ryan W. , Radojevic Bojana , McClard Cynthia K. , Kingsley Ronald , Coussa Razek Georges , Bennett Lea D.
Published: December 2022

The overlapping genetic and clinical spectrum in inherited retinal degeneration (IRD) creates challenges for accurate diagnoses. The goal of this work was to determine the genetic diagnosis and clinical features for patients diagnosed with an IRD. After signing informed consent, peripheral blood or saliva was collected from 64 patients diagnosed with an IRD. Genetic testing was performed on each patient in a Clinical Laboratory Improvement Amendments of 1988 (CLIA) certified laboratory. Mutations were verified with Sanger sequencing and segregation analysis when possible. Visual acuity was measured with a traditional Snellen chart and converted to a logarithm of minimal angle of resolution (logMAR). Fundus images of dilated eyes were acquired with the Optos® camera (Dunfermline, UK). Horizontal line scans were obtained with spectral-domain optical coherence tomography (SDOCT; Spectralis, Heidelberg, Germany). Genetic testing combined with segregation analysis resolved molecular and clinical diagnoses for 75% of patients. Ten novel mutations were found and unique genotype phenotype associations were made for the genes RP2 and CEP83. Collective knowledge is thereby expanded of the genetic basis and phenotypic correlation in IRD.
The Over-Prediction of Seismically Induced Soil Liquefaction during the 2016 Kumamoto, Japan Earthquake Sequence
Authors: Anderson Donald J. , Franke Kevin W. , Kayen Robert E. , Dashti Shideh , Badanagki Mahir
Published: December 2022

Following the M7.0 strike-slip earthquake near Kumamoto, Japan, in April of 2016, most geotechnical engineering experts believed that there would be significant soil liquefaction and liquefaction-induced infrastructure damage observed in the densely populated city of Kumamoto during the post-event engineering reconnaissance. This belief was driven by several factors including the young geologic environment, alluvially deposited soils, a predominance of loose sandy soils documented in publicly available boring logs throughout the region, and the high intensity ground motions observed from the earthquake. To the surprise of many of the researchers, soil liquefaction occurred both less frequently and less severely than expected. This paper summarizes findings from our field, laboratory, and simplified analytical studies common to engineering practice to assess the lower occurrence of liquefaction. Measured in situ SPT and CPT resistance values were evaluated with current liquefaction triggering procedures. Minimally disturbed samples were subjected to cyclic triaxial testing. Furthermore, an extensive literature review on Kumamoto volcanic soils was performed. Our findings suggest that current liquefaction triggering procedures over-predict liquefaction frequency and effects in alluvially deposited volcanic soils. Volcanic soils were found to possess properties of soil crushability, high fines content, moderate plasticity, and unanticipated organic constituents. Cyclic triaxial tests confirm the high liquefaction resistance of these soils. Moving forward, geotechnical engineers should holistically consider the soil’s mineralogy and geology before relying solely on simplified liquefaction triggering procedures when evaluating volcanic soils for liquefaction.
Relationship between Empowerment and Functioning and Disability in Older Japanese Patients: A Covariance Structure Analysis
Authors: Tsubouchi Yoshihito , Tainosho Akiyoshi , Shimomura Koudai , Kou Motoasa , Yorozuya Kyosuke , Nakashima Daiki , Naito Yasuo
Published: December 2022

In the present study, 151 Japanese older adults aged over 65 years and admitted to recovery-phase rehabilitation facilities were enrolled to investigate the relationship between empowerment and contextual factors, functioning and disability, with structural equation modeling (SEM). The analysis included 151 patients aged 81.75 ± 7.15 years, including 54 males (35.76%) and 97 females (64.24%). The results of the SEM analysis showed that role presence (β = 0.45, p < 0.01) and family structure (β = 0.18, p = 0.02) significantly impacted empowerment. In addition, the results showed that patient empowerment positively impacted physical activity (β = 0.25, p < 0.01) and psychosomatic functions and abilities (β = 0.36, p < 0.01). Furthermore, the goodness-of-fit of the model hypothesized in this study was shown to have explanatory power. This study showed that empowerment contributed to the prevention of physical inactivity and confinement among Japanese older patients. In other words, the study provided evidence for the importance of empowerment-based program planning in the practice of person-centered care aimed at promoting the health and discharge of older patients in Japan.
Can Different Admissions to Medical School Predict Performance of Non-Technical Skill Performance in Simulated Clinical Settings?
Authors: Moll-Khosrawi Parisa , Hampe Wolfgang , Schulte-Uentrop Leonie , Zöllner Christian , Zimmermann Stefan , Huelmann Thorben
Published: December 2022

Non-technical skills (NTS) in medical care are essential to ensure patient safety. Focussing on applicants’ NTS during medical school admission could be a promising approach to ensure that future physicians master NTS at a high level. Next to pre-university educational attainment, many selection tests have been developed worldwide to facilitate and standardise the selection process of medical students. The predictive validity of these tests regarding NTS performance in clinical settings has not been investigated (yet). Therefore, we explored the predictive validities and prognosis of the Hamburg MMI (HAM-Int), HAM-Nat, PEA, and waiting as well as other quota (as example) designated by the Federal Armed Forces) for NTS performance in clinical emergency medicine training of medical students. During 2017 and 2020, N = 729 second, third, and fourth year students were enrolled within the study. The mean age of participants was 26.68 years (SD 3.96) and 49% were female students. NTS of these students were assessed during simulation scenarios of emergency training with a validated rating tool. Students admitted via waiting quota and designated by the Armed Forces performed significantly better than students admitted by excellent PEA (p = 0.026). Non-EU students performed significantly inferior (p = 0.003). Our findings provide further insight to explain how and if admission to medical school could predict NTS performance of further physicians.
The Identification of Elderly People with High Fall Risk Using Machine Learning Algorithms
Authors: Lyu Ziyang , Wang Li , Gao Xing , Ma Yingnan
Published: December 2022

Falling is an important public health issue, and predicting the fall risk can reduce the incidence of injury events in the elderly. However, most of the existing studies may have additional human and financial costs for community workers and doctors. Therefore, it is socially important to identify elderly people who are at high fall risk through a reasonable and cost-effective method. We evaluated the potential of multifractal, machine learning algorithms to identify the elderly at high fall risk. We developed a 42-point calibration model of the human body and recorded the three-dimensional coordinate datasets. The stability of the motion trajectory is calculated by the multifractal algorithm and used as an input dimension to compare the performance of the six classifiers. The results showed that the instability of the faller group was significantly greater than that of the no-faller group in the male and female cohorts (p < 0.005), and the Gradient Boosting Decision Tree classifier showed the best performance. The findings could help elderly people at high fall risk to identify individualized risk factors and facilitate tailored fall interventions.
Equilibrium Optimization Algorithm with Ensemble Learning Based Cervical Precancerous Lesion Classification Model
Authors: A. Mansouri Rasha , Ragab Mahmoud
Published: December 2022

Recently, artificial intelligence (AI) with deep learning (DL) and machine learning (ML) has been extensively used to automate labor-intensive and time-consuming work and to help in prognosis and diagnosis. AI’s role in biomedical and biological imaging is an emerging field of research and reveals future trends. Cervical cell (CCL) classification is crucial in screening cervical cancer (CC) at an earlier stage. Unlike the traditional classification method, which depends on hand-engineered or crafted features, convolution neural network (CNN) usually categorizes CCLs through learned features. Moreover, the latent correlation of images might be disregarded in CNN feature learning and thereby influence the representative capability of the CNN feature. This study develops an equilibrium optimizer with ensemble learning-based cervical precancerous lesion classification on colposcopy images (EOEL-PCLCCI) technique. The presented EOEL-PCLCCI technique mainly focuses on identifying and classifying cervical cancer on colposcopy images. In the presented EOEL-PCLCCI technique, the DenseNet-264 architecture is used for the feature extractor, and the EO algorithm is applied as a hyperparameter optimizer. An ensemble of weighted voting classifications, namely long short-term memory (LSTM) and gated recurrent unit (GRU), is used for the classification process. A widespread simulation analysis is performed on a benchmark dataset to depict the superior performance of the EOEL-PCLCCI approach, and the results demonstrated the betterment of the EOEL-PCLCCI algorithm over other DL models.
Clinical Nurses’ Intention to Use Defibrillators in South Korea: A Path Analysis
Authors: Uhm Dongchoon , Jung Gye-Hyun
Published: December 2022

This study aimed to investigate factors affecting behavioral intentions to use defibrillators among clinical nurses in South Korea, using a modified predictive technology acceptance model 2 (TAM 2) that facilitates understanding of what prompts apparent spontaneous actions. This descriptive, cross-sectional study included 467 clinical nurses with more than 6 months of clinical experience. The path analysis results showed that the variables directly affecting the intention to use a defibrillator among clinical nurses were the image, organizational support, perceived ease of use, and perceived usefulness. Moreover, job autonomy and total career indirectly affected the intention to use a defibrillator. Clinical nurses need to know how to use defibrillators and be able to act promptly on patients with in-hospital cardiac arrest (IHCA). Organizational managers need to improve the work environments of clinical nurses accordingly. Additionally, it is necessary to establish a policy foundation to ensure the development of competence and job autonomy that can lead to the use of defibrillators by improving various factors, including anxiety or reluctance of nurses to use defibrillators in acute cardiac arrest.
Factorial Structure and Psychometric Properties of the Spanish Version of the Pittsburgh Sleep Quality Index in Non-Professional Caregivers
Authors: Otero Patricia , Simón Miguel A. , Bueno Ana M. , Blanco Vanessa , Vázquez Fernando L.
Published: December 2022

Although sleep issues are among the symptoms commonly experienced by the non-professional caregiver population, and the Pittsburgh Sleep Quality Index (PSQI) is the most widely used instrument for the assessment of sleep quality, this has not been validated specifically for this population. The objective of this study was to analyze the factorial structure and psychometric properties of the Spanish version of the PSQI in a sample of Spanish non-professional caregivers. Trained clinical psychologists assessed sleep quality using the PSQI, as well as caregiver burden and psychological distress in 201 non-professional caregivers (87.1% female, Mage = 56.2 years). The internal consistency of the PSQI was 0.75. The two-factor model (Sleep quality and Disturbances) had an acceptable fit to the data, was found to be superior to the one-factor model, and more parsimonious than the three-factor model. There was a significant correlation between the PSQI and caregiver burden, as well as between the PSQI and psychological distress (p < 0.001 in all cases). A total score ≥ 9 allowed the identification of caregivers with possible anxiety and depression disorders (sensitivity 70.5%, specificity 71.9%). The results show that the PSQI is a reliable and valid instrument for the assessment of sleep quality in caregivers.
The Impact of Chronic Suppurative Otitis Media with and without Cholesteatoma in Patients from Northeastern Romania
Authors: Serban Roxana , Frasinariu Otilia Elena , Simionescu Bianca , Radulescu Luminita Mihaela , Cobzeanu Bogdan Mihail , Filip Cristiana , Buga Ana Maria Laura , Pohaci Antonesei Luiza Simona , Temneanu Oana Raluca
Published: December 2022

Quality of life is a widely used concept that tends to become an important part of clinical management. The present study performs an analysis of the impact of suppurative chronic otitis media with and without cholesteatoma on quality of life, using the COMQ-12 questionnaire. It was applied to a group of 40 healthy people and to 40 patients before surgery, and the answers to the questions were analyzed and correlated with socioeconomic factors. After the confirmation of the diagnosis based on clinical and imaging information, the patients completed the COMQ-12 questionnaire. It was observed that the chronic ear problems had negative impacts of varying degrees on daily and long-term activities. The evaluation and analysis of information can be used in setting therapeutic targets.
What Is the Impact of Leaky Sewers on Groundwater Contamination in Urban Semi-Confined Aquifers? A Test Study Related to Fecal Matter and Personal Care Products (PCPs)
Authors: Ducci Laura , Rizzo Pietro , Pinardi Riccardo , Solfrini Augusto , Maggiali Alessandro , Pizzati Mattia , Balsamo Fabrizio , Celico Fulvio
Published: December 2022

Urban areas exercise numerous and strong pressures on water bodies, implying that different external anthropogenic factors also stress groundwater. Sewerage networks play an important role, being the place of wastewater flow. When sewerage deterioration conditions occur, aquifers can be contaminated by contaminants contained within wastewater. The study aims to verify the impact of sewerage leaks in urban semi-confined aquifers through a multidisciplinary approach. Geological, hydrogeological, hydrochemical, microbiological, and biomolecular investigations are carried out in a test site close to a sewer pipe, from February to October 2022. Microbiological analyses are carried out on a monthly basis, contextually to hydraulic head measurements in purpose-drilled piezometers. The presence of sandy intercalations and the prevalence of silt within the outcropping (about 10 m thick) aquitard makes the aquifer vulnerable to percolation from leaky sewers, therefore causing persistent microbial contamination in groundwater. The presence of fecal indicators (including pathogenic genera), corrosive and human-associated bacteria markers, is detected. The magnitude of microbiological impact varies over time, depending on hydrogeological factors such as dilution, hydrodynamic dispersion, and variation of the groundwater flow pathway at the site scale. As for personal care products, only Disodium EDTA is detected in wastewater, while in groundwater the concentrations of all the analyzed substances are lower than the instrumental detection limit.
Leptospira Seroprevalence in Free-Ranging Long-Tailed Macaques (Macaca fascicularis) at Kosumpee Forest Park, Maha Sarakham, Thailand
Authors: Pumipuntu Natapol , Tanee Tawatchai , Kyes Pensri , Thamsenanupap Penkhae , Karaket Apichat , Kyes Randall C.
Published: December 2022

Background: Leptospirosis is a zoonotic disease that is ubiquitously distributed and is classified as a re-emerging infectious disease in humans and animals. Many serovars are carried by wildlife; all of them are capable of causing illness in humans. The purpose of this study was to investigate the prevalence of Leptospirosis in wild long-tailed macaques (Macaca fascicularis) at Kosumpee Forest Park, Mahasarakham, Thailand. Methods: A cross-sectional study was conducted at the park. Blood samples were collected via saphenous vein from 30 free-ranging long-tailed macaques. All samples were tested by the microscopic agglutination test. The LipL32 gene was used to detect pathogenic Leptospira in blood samples by conventional polymerase chain reaction. Results: Screening of the 30 wild macaques showed an overall Leptospira seroreactivity of 13.33%. Three of 30 macaques reacted against Leptospira serovar Shermani and one macaque was infected with Leptospira serovar Sejroe. None of the macaques presented clinical signs of leptospirosis. None of the blood samples showed the detection of the LipL32 gene. Conclusions: The results indicate that the long-tailed macaques at Kosumpee Forest Park may act as natural reservoirs for Leptospirosis. Further, the results provide evidence-based information indicating that several pathogenic Leptospira serovars are circulating in the wild macaques in the study area.
Factors Associated with Self-Report of Type 2 Diabetes Mellitus in Adults Seeking Dental Care in a Developing Country
Authors: López-Gómez Sandra Aremy , González-López Blanca Silvia , Scougall-Vilchis Rogelio José , Márquez-Corona María de Lourdes , Minaya-Sánchez Mirna , Navarrete-Hernández José de Jesús , de la Rosa-Santillana Rubén , Acuña-González Gladys Remigia , Pontigo-Loyola América Patricia , Villalobos-Rodelo Juan José , Medina-Solís Carlo Eduardo , Maupomé Gerardo
Published: December 2022

The aims of the present study were to identify the prevalence and risk indicators of type 2 diabetes mellitus (T2DM) in urban-based Mexican adults seeking care in a university-based triage/intake dental clinic, and to develop a predictive model. A cross-sectional study was conducted on 3354 medical/dental records of adults who sought care at the triage/intake dental clinics of a public university. The dependent variable was self-report of a previous diagnosis of T2DM made by a physician. Several socio-demographic and socioeconomic covariates were included, as well as others related to oral and general health. A multivariate binary logistic regression model was generated. We subsequently calculated well-known statistical measures employed to evaluate discrimination (classification) using an (adjusted) multivariate logistic regression model (goodness-of-fit test). The average age of patients was 42.5 ± 16.1 years old and the majority were female (64.1%). The prevalence of T2DM was 10.7% (95%CI = 9.7–11.8). In the final multivariate model, the variables associated (p < 0.05) with the presence of T2DM were older age (40 to 59 years old, OR = 2.00; 60 to 95 years old, OR = 2.78), having any type of health insurance (OR = 2.33), having high blood pressure (OR = 1.70), being obese (OR = 1.41), and having a functional dentition (OR = 0.68). Although the global fit of the model and the calibration tests were adequate, the sensitivity (0.0%) and positive predictive (0.0%) values were not. The specificity (100%) and negative predictive (89.3%) values, as well as the correctly classified (89.3%) value, were adequate. The area under the ROC curve, close to 0.70, was modest. In conclusion, a prevalence of T2DM of 10.7% in this sample of Mexican adults seeking dental care was similar to national figures. Clinical (blood pressure, BMI and functional dentition), demographic (age), and socioeconomic (health insurance) variables were found to be associated with T2DM. The dental setting could be appropriate for implementing preventive actions focused on identifying and helping to reduce the burden of T2DM in the population.
Indigenous Knowledge: Revitalizing Everlasting Relationships between Alaska Natives and Sled Dogs to Promote Holistic Wellbeing
Authors: Newman Janessa , Rivkin Inna , Brooks Cathy , Turco Kathy , Bifelt Joseph , Ekada Laura , Philip Jacques
Published: December 2022

Introduction: Indigenous peoples have documented their culture’s history in oral stories, revealing lessons about holistic relationships fostering perseverance. Despite vast differences in time, relationships and stories are equally important today. Athabascans retain their values, life skills, and wellness through cultural practices. Creating opportunities for youths to learn through reciprocal relationships increases wellness in Indigenous communities, highlighting the significance of community-developed programs that connect youths to their place and culture. Method: Athabascan youths in rural Alaska get hands-on experience and Elder mentorship working with sled dogs in the Frank Attla Youth and Sled Dog Care-Mushing Program (FAYSDP). Through a community-based participatory research partnership with the community of Huslia and Jimmy Huntington School, we examined how FAYSDP affects youths, and how relationships within culture and land promote wellbeing. Fifteen middle and high-school youths shared their perspectives on how FAYSDP impacts them and their community using photovoice and digital storytelling. Nineteen adults contributed their perspectives in focus groups. We used emerging themes (inductive) and Athabascan cultural values and elements of social capital (deductive approaches) to analyze the qualitative data. Results: Findings illustrate how FAYSDP promotes wellbeing by empowering youths to apply what they learn to generate their own knowledge, while centering communities around culturally significant practices such as dog mushing. It connects youths to their home and their cultural values, using dogs as the driving force to bridge generations and foster youth wellness. Discussion: We discuss implications for community-based programs that engage Elders as teachers and the land as their classroom to promote youth holistic wellness.
Study on the Status of Scientific Fitness Literacy of Rural Left-Behind Minors and the Influence of Family Environment
Authors: Pan Xiang , Gao Yibo , Jiang Lupei , Deng Pengyu , He Jin , Xia Tian , Zhang Aoyu , Zhang Yanfeng
Published: December 2022

There are a large number of 6.97 million left-behind children in China at the end of August 2018. Left-behind children exhibit many psychologically unhealthy behavioural disorders. This study aimed to compare the differences in scientific fitness literacy (SFL) between rural left-behind and general minors, and to analyze the factors affecting the SFL of left-behind minors in the family environment. A random stratified sampling was conducted among minors aged 3–18 in rural areas of China, and 2239 valid samples were obtained. The questionnaire is based on the SFL part and the family environment part of the China National Fitness Activity Status Survey, except for the SFL part of the children’s questionnaire does not contain the attitude dimension, the questionnaires of several other age groups contain three dimensions: attitude, skills, and habits. The SFL of general children and adolescents was higher than that of left-behind children and adolescents, and in the sub-dimensions, children’s attitudes and adolescents’ skills and habits differed greatly, with the general minors having better performance. The SFL showed a significant increasing trend with age in the general minors but the difference between children and adolescents in the left-behind minors was not significant. The scores of habituation in left-behind minors increased with age group, and the scores of the general minors group did not vary much between age groups. In the multiple regression analysis for the left-behind minors, it was found that left-behind young children were less influenced by family environment on SFL score, and children’s SFL was more influenced by family environment. A separate analysis of the 7–9 and 10–12-year-old groups revealed that factors such as parental support and encouragement influenced SFL with different weights, and the importance of parents rotated, while the number of sporting goods in the family was important in all four age models. For the left-behind minors with a large amount in China, the role of the family environment in their SFL cannot be ignored, and the promotion of this literacy should be carried out in a targeted manner.
The Relationships among Sport Participation Level, Flow Experience, Perceived Health Status and Depression Level of College Students
Authors: Lin Suh-Ting , Hung Ying-Hua , Yang Meng-Hua
Published: December 2022

The purpose of this study was to examine the relationships between sport participation level, flow, perceived health status and depression using gender and grades as control variables of college students in Taiwan. Based on previous research, the study established the proposed model: using sport participation level and flow experience as predicting variables, perceived health status and depression as dependent variables, and gender and grades as control variables. A total of 700 structured questionnaires were distributed to college students using convenience sampling among seven universities in Taiwan with a valid return rate of 86.5%. Structural equation modeling was used to test the relationships among the above-mentioned variables. The study found: 1. Male students had higher self-rated health perception than female students. 2. Students with higher grades perceived higher levels of depression than those with lower grades. 3. Among all variables, the level of sport participation had a positive predicting power of perceived health status and a negative predicting power of depression level; perceived health status had a negative predicting power of depression; while flow had no moderating effect among sport participation level, perceived health status and depression. In the model, the predicting variables had a predicting power of 0.58 (R2) for depression, indicating a good model. Conclusions and implications were made according to the findings of the study.