bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Cephalosporin Treatment on the Microbiota and Antibiotic Resistance Genes in Feces of Dairy Cows with Clinical Mastitis
Authors: Dong Lei , Meng Lu , Liu Huimin , Wu Haoming , Schroyen Martine , Zheng Nan , Wang Jiaqi
Published: January 2022

Antibiotics are frequently used to treat dairy cows with mastitis. However, the potential effects of β-lactam antibiotics, such as cephalosporins, on the fecal microbiome is unknown. The objective was to investigate the effects of ceftiofur and cefquinome on the fecal microbiota and antibiotic resistance genes of dairy cows with mastitis. The fecal samples were collected from 8 dairy cows at the following periods: the start day (Day 0), medication (Days 1, 2, and 3), withdrawal (Days 4, 6, 7, and 8), and recovery (Days 9, 11, 13, and 15). 16S rRNA gene sequencing was applied to explore the changes in microbiota, and qPCR was used to investigate the antibiotic resistance genes. The cephalosporin treatment significantly decreased the microbial diversity and richness, indicated by the decreased Shannon and Chao 1 indexes, respectively (p < 0.05). The relative abundance of Bacteroides, Bacteroidaceae, Bacteroidales, and Bacteroidia increased, and the relative abundance of Clostridia, Clostridiales, Ethanoligenens, and Clostridium IV decreased at the withdrawal period. The cephalosporin treatment increased the relative abundance of β-lactam resistance genes (blaTEM and cfxA) at the withdrawal period (p < 0.05). In conclusion, the cephalosporin treatment decreased the microbial diversity and richness at the medication period, and increased the relative abundance of two β-lactam resistance genes at the withdrawal period.
Home Oral Care of Periodontal Patients Using Antimicrobial Gel with Postbiotics, Lactoferrin, and Aloe Barbadensis Leaf Juice Powder vs. Conventional Chlorhexidine Gel: A Split-Mouth Randomized Clinical Trial
Authors: Butera Andrea , Gallo Simone , Pascadopoli Maurizio , Taccardi Damiano , Scribante Andrea
Published: January 2022

Periodontitis is a progressive destruction of both soft and hard tooth-supporting tissues. In the last years, probiotics have been proposed as a support to the gold standard treatment scaling and root planing (SRP), but no extensive literature is present as regards the effect of the more recent postbiotics. Thirty patients subjected to SRP were randomly assigned to two domiciliary hygiene treatments based on the following oral gels: the postbiotics-based Biorepair Parodontgel Intensive (Group 1) and the chlorhexidine-based Curasept Periodontal Gel (Group 2). At baseline (T0) and after 3 and 6 months (T1–T2), the following periodontal clinical parameters were recorded: Probing Pocket Depth (PPD), recession, dental mobility, Bleeding on Probing (BoP), and Plaque Control Record (PCR). A significant intragroup reduction was assessed in both groups for PPD, BoP, and PCR; conversely, recession significantly increased in both groups, whereas dental mobility did not vary. As regards intergroup comparisons, no statistically significant differences were assessed. Both gels, respectively, containing antioxidant natural ingredients and chlorhexidine, are effective for the domiciliary treatment of periodontitis. Further studies are required to evaluate the singular chemical compounds of the gels expected to exert the beneficial action assessed in this preliminary study.
Therapeutic Strategies Targeting Mitochondrial Calcium Signaling: A New Hope for Neurological Diseases?
Authors: Rodríguez Laura R. , Lapeña-Luzón Tamara , Benetó Noelia , Beltran-Beltran Vicent , Pallardó Federico V. , Gonzalez-Cabo Pilar , Navarro Juan Antonio
Published: January 2022

Calcium (Ca2+) is a versatile secondary messenger involved in the regulation of a plethora of different signaling pathways for cell maintenance. Specifically, intracellular Ca2+ homeostasis is mainly regulated by the endoplasmic reticulum and the mitochondria, whose Ca2+ exchange is mediated by appositions, termed endoplasmic reticulum–mitochondria-associated membranes (MAMs), formed by proteins resident in both compartments. These tethers are essential to manage the mitochondrial Ca2+ influx that regulates the mitochondrial function of bioenergetics, mitochondrial dynamics, cell death, and oxidative stress. However, alterations of these pathways lead to the development of multiple human diseases, including neurological disorders, such as amyotrophic lateral sclerosis, Friedreich’s ataxia, and Charcot–Marie–Tooth. A common hallmark in these disorders is mitochondrial dysfunction, associated with abnormal mitochondrial Ca2+ handling that contributes to neurodegeneration. In this work, we highlight the importance of Ca2+ signaling in mitochondria and how the mechanism of communication in MAMs is pivotal for mitochondrial maintenance and cell homeostasis. Lately, we outstand potential targets located in MAMs by addressing different therapeutic strategies focused on restoring mitochondrial Ca2+ uptake as an emergent approach for neurological diseases.
Inhibitory Effects of 6,8-Diprenylorobol on Endometriosis Progression in Humans by Disrupting Calcium Homeostasis and Mitochondrial Function
Authors: Song Jisoo , Song Gwonhwa , Park Sunwoo , Lim Whasun
Published: January 2022

6,8-Diprenylorobol is a flavonoid compound extracted from Cudrania tricuspidata. It has various biological functions, such as inhibiting melanin synthesis and inducting cell death in cancerous cells. In addition, Cudrania tricuspidata is known to be effective in female diseases, and previous studies have shown anticancer effects in cervical cancer, a female reproductive disease. Outside of that, Cudrania tricuspidata has various physiological effects. However, the effect of 6,8-diprenylorobol is not well known in other benign and chronic diseases, even in endometriosis, which commonly arises in the female reproductive tract. In the present study, we determined the inhibitory effects of 6,8-diprenylorobol on the growth of endometriosis VK2/E6E7 and End1/E6E7 cells. Results indicated that 6,8-diprenylorobol suppressed cellular proliferation and increased the disruption of the cell cycle, mitochondrial membrane potential (MMP), generation of reactive oxygen species, and Ca2+ homeostasis in both endometriosis cells. However, the proliferation of normal stromal cells isolated from endometrial tissue was not altered by 6,8-diprenylorobol. The change in Ca2+ levels was estimated in fluo-4- or rhod-2-stained VK2/E6E7 and End1/E6E7 cells after the treatment of the intracellular calcium regulators 2-aminoethoxydiphenyl borate (2-APB) and ruthenium red (RUR) with 6,8-diprenylorobol. A combination of 6,8-diprenylorobol with each regulator decreased the calcium accumulation in endometriosis cells. Furthermore, Western blot analysis indicated that 6,8-diprenylorobol inactivated AKT pathways, whereas it activated P38 MAPK pathways. In addition, 6,8-diprenylorobol decreased mitochondrial respiration, leading to the reduction in ATP production in VK2/E6E7 and End1/E6E7 cells. Collectively, our results suggested that 6,8-diprenylorobol might be a potential therapeutic agent or adjuvant therapy for the management of endometriosis.
Seaweed-Derived Proteins and Peptides: Promising Marine Bioactives
Authors: Echave Javier , Otero Paz , Garcia-Oliveira Paula , Munekata Paulo E. S. , Pateiro Mirian , Lorenzo Jose M. , Simal-Gandara Jesus , Prieto Miguel A.
Published: January 2022

Seaweeds are a typical food of East-Asian cuisine, to which are alleged several beneficial health effects have been attributed. Their availability and their nutritional and chemical composition have favored the increase in its consumption worldwide, as well as a focus of research due to their bioactive properties. In this regard, seaweed proteins are nutritionally valuable and comprise several specific enzymes, glycoproteins, cell wall-attached proteins, red algae phycobiliproteins, lectins, peptides, or mycosporine-like amino acids. This great extent of molecules has been reported to exert significant antioxidant, antimicrobial, anti-inflammatory, antihypertensive, antidiabetic, or antitumoral properties. Hence, knowledge on algae proteins and derived compounds have gained special interest for the potential nutraceutical, cosmetic or pharmaceutical industries based on these bioactivities. Although several molecular mechanisms of action on how these proteins and peptides exert biological activities have been described, many gaps in knowledge still need to be filled. Updating the current knowledge related to seaweed proteins and peptides is of interest to further asses their potential health benefits. This review addresses the characteristics of seaweed protein and protein-derived molecules, their natural occurrence, their studied bioactive properties, and their described potential mechanisms of action.
Performance Analysis of a Waste-to-Energy System Integrated with the Steam–Water Cycle and Urea Hydrolysis Process of a Coal-Fired Power Unit
Authors: Zhang Yuanyuan , Wei Lai , Gao Xin , Chen Heng , Li Qiubai , Zhang Kai , Huang Qilong
Published: January 2022

An innovative hybrid energy system consisting of a waste-to-energy unit and a coal-fired power unit is designed to enhance the energy recovery of waste and decrease the investment costs of waste-to-energy unit. In this integrated design, partial cold reheat steam of the coal-fired unit is heated by the waste-to-energy boiler’s superheater. The heat required for partial preheated air of waste-to-energy unit and its feedwater are supplied by the feedwater of CFPU. In addition, an additional evaporator is deployed in the waste-to-energy boiler, of which the outlet stream is utilized to provide the heat source for the urea hydrolysis unit of coal-fired power plant. The stand-alone and proposed designs are analyzed and compared through thermodynamic and economic methods. Results indicate that the net total energy efficiency increases from 41.84% to 42.12%, and the net total exergy efficiency rises from 41.19% to 41.46% after system integration. Moreover, the energy efficiency and exergy efficiency of waste-to-energy system are enhanced by 10.48% and 9.92%, respectively. The dynamic payback period of new waste-to-energy system is cut down from 11.39 years to 5.48 years, and an additional net present value of $14.42 million is got than before.
High-Temperature Superconductivity in the Lanthanide Hydrides at Extreme Pressures
Authors: Wei Yao , Macheda Francesco , Zhao Zelong , Tse Terence , Plekhanov Evgeny , Bonini Nicola , Weber Cedric
Published: January 2022

Hydrogen-rich superhydrides are promising high-Tc superconductors, with superconductivity experimentally observed near room temperature, as shown in recently discovered lanthanide superhydrides at very high pressures, e.g., LaH10 at 170 GPa and CeH9 at 150 GPa. Superconductivity is believed to be closely related to the high vibrational modes of the bound hydrogen ions. Here, we studied the limit of extreme pressures (above 200 GPa) where lanthanide hydrides with large hydrogen content have been reported. We focused on LaH16 and CeH16, two prototype candidates for achieving a large electronic contribution from hydrogen in the electron–phonon coupling. In this work, we propose a first-principles calculation platform with the inclusion of many-body corrections to evaluate the detailed physical properties of the Ce–H and La–H systems and to understand the structure, stability, and superconductivity of these systems at ultra-high pressure. We provide a practical approach to further investigate conventional superconductivity in hydrogen-rich superhydrides. We report that density functional theory provides accurate structure and phonon frequencies, but many-body corrections lead to an increase of the critical temperature, which is associated with the spectral weight transfer of the f-states.
Evaluation of Different Internal Designs of Hydraulic Nozzles under an Accelerated Wear Test
Authors: Milanowski Marek , Subr Alaa , Parafiniuk Stanisław
Published: January 2022

The use of worn-out agricultural nozzles in pesticide application has a negative effect on the efficiency and cost of the application process. It also has an effect on environmental pollution due to an excessive amount of pesticide being applied when spraying with worn-out nozzles. In this paper, the resistance to wear of three different internal design hydraulic nozzles was ascertained. Changes in the flow rate and spray distribution as a result of this wear were also investigated. The wear test was done inside a closed system, and it was accelerated using an abrasive material to generate 100 h of wear. The tested nozzles were the Turbo TeeJet (TT)-twin chambered, Turbo Twinjet (TTj60)-dual outlet, and Drift Guard (DG)-pre-orifice. Wear rate, flow rate, and the virtual coefficient of variation (CVv) were measured at different wear intervals. The results showed that the TTj60 type was the most resistant to wear, followed by the TT type and DG. The latter two types showed an increase in the flow rate only in the first 45 h of wear. Virtual coefficient of variation (CVv) values were less than 10% after finishing the test (after 100 h of wear) for the three types of nozzles, which are acceptable values according to International Organization for Standardization (ISO) 16122-2, 2015.
Age and Gender Impact on Password Hygiene
Authors: Juozapavičius Aušrius , Brilingaitė Agnė , Bukauskas Linas , Lugo Ricardo Gregorio
Published: January 2022

Password hygiene plays an essential part in securing systems protected with single-factor authentication. A significant fraction of security incidents happen due to weak or reused passwords. The reasons behind differences in security vulnerable behaviour between various user groups remains an active research topic. The paper aims to identify the impact of age and gender on password strength using a large password dataset. We recovered previously hashed passwords of 102,120 users from a leaked customer database of a car-sharing company. Although the measured effect size was small, males significantly had stronger passwords than females for all age groups. Males aged 26–45 were also significantly different from all other groups, and password complexity decreased with age for both genders equally. Overall, very weak password hygiene was observed, 72% of users based their password on a word or used a simple sequence of digits, and passwords of over 39% of users were found in word lists of previous leaks.
Metrology for Measuring Bumps in a Protection Layer Based on Phase Shifting Fringe Projection
Authors: Ku Yi-Sha , Chang Po-Yi , Lee Han-Wen , Lo Chun-Wei , Chen Yi-Chang , Cho Chia-Hung
Published: January 2022

We developed a telecentric 3D measurement system based on the fringe projection technique to measure phases corresponding to the 3D shape of bumps on a surface. A measurement algorithm based on an area scan camera with a flexibly programmable region of interest (ROI) was applied to reconstruct the 3D model. Only the pixel information within the ROI is stored, which reduces the data volume and processing and accelerates the area scan acquisition. Experiments showed that the fringe projection system provided good linearity between the calibration and optical data with excellent accuracy. A reflectometry sensor was implemented to provide a correction offset to the bump height in a protection layer in a subsequent high-speed inspection.
A Fluid Inclusion and Critical/Rare Metal Study of Epithermal Quartz-Stibnite Veins Associated with the Gerakario Porphyry Deposit, Northern Greece
Authors: Stergiou Christos L. , Melfos Vasilios , Voudouris Panagiotis , Papadopoulou Lambrini , Spry Paul G. , Peytcheva Irena , Dimitrova Dimitrina , Stefanova Elitsa
Published: January 2022

The Gerakario Cu-Au porphyry deposit in the Kilkis ore district, northern Greece, contains epithermal quartz-stibnite veins on the eastern side of the deposit, which crosscut a two-mica gneiss. Metallic mineralization in these veins consists of stibnite + berthierite + native antimony + pyrite + arsenopyrite, and minor marcasite, pyrrhotite, chalcopyrite, löllingite, and native gold. Bulk geochemical analyses of the ore reveal an enrichment in critical and rare metals, including Ag, Au, Bi, Ce, Co, Ga, La, and Sb. Analysis of stibnite with LA-ICP-MS showed an enrichment in base metals (As, Cu, Pb), as well as weak to moderate contents of critical and rare metals (Ag, Bi, Ce, La, Re, Sm, Th, Ti, Tl). A statistical analysis of the trace elements show a positive correlation for the elemental pairs Ce-La, Ce-Sb, and La-Sb, and a negative correlation for the pair Bi-Sb. Fluid inclusions in the A-type veins of the porphyry-style mineralization show the presence of fluid boiling, resulting in a highly saline aqueous fluid phase (35.7 to 45.6 wt.% NaCl equiv.) and a moderately saline gas phase (14 to 22 wt.% NaCl equiv.) in the system H2O-NaCl-KCl at temperatures varying between 380° and 460 °C and pressures from 100 to 580 bar. Mixing of the moderate saline fluid with meteoric water produced less saline fluids (8 to 10 wt.% NaCl equiv.), which are associated with the epithermal quartz-stibnite vein mineralization. This process took place under hydrostatic pressures ranging from 65 to 116 bar at a depth between 600 and 1000 m, and at temperatures mainly from 280° to 320 °C.
Characterizing the Mechanical Performance of a Bare-Metal Stent with an Auxetic Cell Geometry
Authors: Bhullar Sukhwinder K. , Lekesiz Huseyin , Karaca Ahmet Abdullah , Cho Yonghyun , Willerth Stephanie Michelle , Jun Martin B. G.
Published: January 2022

This study develops and characterizes the distinctive mechanical features of a stainless-steel metal stent with a tailored structure. A high-precision femtosecond laser was used to micromachine a stent with re-entrant hexagonal (auxetic) cell geometry. We then characterized its mechanical behavior under various mechanical loadings using in vitro experiments and through finite element analysis. The stent properties, such as the higher capability of the stent to bear upon bending, exceptional advantage at elevated levels of twisting angles, and proper buckling, all ensured a preserved opening to maintain the blood flow. The outcomes of this preliminary study present a potential design for a stent with improved physiologically relevant mechanical conditions such as longitudinal contraction, radial strength, and migration of the stent.
Phytochemical Analysis of Polyphenols in Leaf Extract from Vernonia amygdalina Delile Plant Growing in Uganda
Authors: Nowak Jadwiga , Kiss Anna K. , Wambebe Charles , Katuura Esther , Kuźma Łukasz
Published: January 2022

Due to the presence of phytochemicals, plants have been known to be used in the treatment and management of various diseases. Vernonia amygdalina, belonging to the Asteraceae family, is a plant known for its many applications in traditional medicine for various purposes. Previous studies on the methanolic leaf extract of this plant have proved the antibacterial, cytotoxic, anticancer and antioxidant effects indicative of promising therapeutic potentials. In this work, chromatographic and spectroscopic techniques along with high-performance liquid chromatography quantitative analysis were adopted to isolate, identify and quantify polyphenolic compounds in V. amygdalina leaf extract. UHPLC-DAD-ESI-MS/MS and UHPLC-DAD methods were adopted for qualitative and quantitative analysis, respectively. In the case of polyphenol separation, some reference substances were isolated by preparative HPLC. Seven polyphenols were identified and quantified in this study: 5-O-caffeoylquinic acid, luteolin hexoside, 3,4-O-dicaffeoylquinic acid, 1,5-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid and luteolin dihexoside, with 3,5-O-dicaffeoylquinic acid being isolated in the highest quantity of 27.49 mg g−1 extract.
Different Scales of Medical Data Classification Based on Machine Learning Techniques: A Comparative Study
Authors: Elzeheiry Heba Aly , Barakat Sherief , Rezk Amira
Published: January 2022

In recent years, medical data have vastly increased due to the continuous generation of digital data. The different forms of medical data, such as reports, textual, numerical, monitoring, and laboratory data generate the so-called medical big data. This paper aims to find the best algorithm which predicts new medical data with high accuracy, since good prediction accuracy is essential in medical fields. To achieve the study’s goal, the best accuracy algorithm and least processing time algorithm are defined through an experiment and comparison of seven different algorithms, including Naïve bayes, linear model, regression, decision tree, random forest, gradient boosted tree, and J48. The conducted experiments have allowed the prediction of new medical big data that reach the algorithm with the best accuracy and processing time. Here, we find that the best accuracy classification algorithm is the random forest with accuracy values of 97.58%, 83.59%, and 90% for heart disease, M-health, and diabetes datasets, respectively. The Naïve bayes has the lowest processing time with values of 0.078, 7.683, and 22.374 s for heart disease, M-health, and diabetes datasets, respectively. In addition, the best result of the experiment is obtained by the combination of the CFS feature selection algorithm with the Random Forest classification algorithm. The results of applying RF with the combination of CFS on the heart disease dataset are as follows: Accuracy of 90%, precision of 83.3%, sensitivity of 100, and consuming time of 3 s. Moreover, the results of applying this combination on the M-health dataset are as follows: Accuracy of 83.59%, precision of 74.3%, sensitivity of 93.1, and consuming time of 13.481 s. Furthermore, the results on the diabetes dataset are as follows: Accuracy of 97.58%, precision of 86.39%, sensitivity of 97.14, and consuming time of 56.508 s.
A Phase-Field Perspective on Mereotopology
Authors: Schmitz Georg J.
Published: January 2022

Mereotopology is a concept rooted in analytical philosophy. The phase-field concept is based on mathematical physics and finds applications in materials engineering. The two concepts seem to be disjoint at a first glance. While mereotopology qualitatively describes static relations between things, such as x isConnected y (topology) or x isPartOf y (mereology) by first order logic and Boolean algebra, the phase-field concept describes the geometric shape of things and its dynamic evolution by drawing on a scalar field. The geometric shape of any thing is defined by its boundaries to one or more neighboring things. The notion and description of boundaries thus provides a bridge between mereotopology and the phase-field concept. The present article aims to relate phase-field expressions describing boundaries and especially triple junctions to their Boolean counterparts in mereotopology and contact algebra. An introductory overview on mereotopology is followed by an introduction to the phase-field concept already indicating its first relations to mereotopology. Mereotopological axioms and definitions are then discussed in detail from a phase-field perspective. A dedicated section introduces and discusses further notions of the isConnected relation emerging from the phase-field perspective like isSpatiallyConnected, isTemporallyConnected, isPhysicallyConnected, isPathConnected, and wasConnected. Such relations introduce dynamics and thus physics into mereotopology, as transitions from isDisconnected to isPartOf can be described.
A Model of Directed Graph Cofiber
Authors: McGuirk Zachary , Park Byungdo
Published: January 2022

In the homotopy theory of spaces, the image of a continuous map is contractible to a point in its cofiber. This property does not apply when we discretize spaces and continuous maps to directed graphs and their morphisms. In this paper, we give a construction of a cofiber of a directed graph map whose image is contractible in the cofiber. Our work reveals that a category-theoretically correct construction in continuous setup is no longer correct when it is discretized and hence leads to look at canonical constructions in category theory in a different perspective.
Extraction of the Relations among Significant Pharmacological Entities in Russian-Language Reviews of Internet Users on Medications
Authors: Sboev Alexander , Selivanov Anton , Moloshnikov Ivan , Rybka Roman , Gryaznov Artem , Sboeva Sanna , Rylkov Gleb
Published: January 2022

Nowadays, the analysis of digital media aimed at prediction of the society’s reaction to particular events and processes is a task of a great significance. Internet sources contain a large amount of meaningful information for a set of domains, such as marketing, author profiling, social situation analysis, healthcare, etc. In the case of healthcare, this information is useful for the pharmacovigilance purposes, including re-profiling of medications. The analysis of the mentioned sources requires the development of automatic natural language processing methods. These methods, in turn, require text datasets with complex annotation including information about named entities and relations between them. As the relevant literature analysis shows, there is a scarcity of datasets in the Russian language with annotated entity relations, and none have existed so far in the medical domain. This paper presents the first Russian-language textual corpus where entities have labels of different contexts within a single text, so that related entities share a common context. therefore this corpus is suitable for the task of belonging to the medical domain. Our second contribution is a method for the automated extraction of entity relations in Russian-language texts using the XLM-RoBERTa language model preliminarily trained on Russian drug review texts. A comparison with other machine learning methods is performed to estimate the efficiency of the proposed method. The method yields state-of-the-art accuracy of extracting the following relationship types: ADR–Drugname, Drugname–Diseasename, Drugname–SourceInfoDrug, Diseasename–Indication. As shown on the presented subcorpus from the Russian Drug Review Corpus, the method developed achieves a mean F1-score of 80.4% (estimated with cross-validation, averaged over the four relationship types). This result is 3.6% higher compared to the existing language model RuBERT, and 21.77% higher compared to basic ML classifiers.
Whole-Cell Display of Phosphotransferase in Escherichia coli for High-Efficiency Extracellular ATP Production
Authors: Zhao Shuai , Yang Guoli , Xie Xiaochen , Yan Guangbo , Wang Fei , Chen Wanping , Ma Lixin
Published: January 2022

Adenosine triphosphate (ATP), as a universal energy currency, takes a central role in many biochemical reactions with potential for the synthesis of numerous high-value products. However, the high cost of ATP limits industrial ATP-dependent enzyme-catalyzed reactions. Here, we investigated the effect of cell-surface display of phosphotransferase on ATP regeneration in recombinant Escherichia coli. By N-terminal fusion of the super-folder green fluorescent protein (sfGFP), we successfully displayed the phosphotransferase of Pseudomonas brassicacearum (PAP-Pb) on the surface of E. coli cells. The catalytic activity of sfGFP-PAP-Pb intact cells was 2.12 and 1.47 times higher than that of PAP-Pb intact cells, when the substrate was AMP and ADP, respectively. The conversion of ATP from AMP or ADP were up to 97.5% and 80.1% respectively when catalyzed by the surface-displayed enzyme at 37 °C for only 20 min. The whole-cell catalyst was very stable, and the enzyme activity of the whole cell was maintained above 40% after 40 rounds of recovery. Under this condition, 49.01 mg/mL (96.66 mM) ATP was accumulated for multi-rounds reaction. This ATP regeneration system has the characteristics of low cost, long lifetime, flexible compatibility, and great robustness.
Detection Rate and Clinical Impact of PET/CT with 18F-FACBC in Patients with Biochemical Recurrence of Prostate Cancer: A Retrospective Bicentric Study
Authors: Filippi Luca , Bagni Oreste , Crisafulli Carmelo , Cerio Ivan , Brunotti Gabriele , Chiaravalloti Agostino , Schillaci Orazio , Dore Franca
Published: January 2022

Our aim was to assess the detection rate (DR) of positron emission computed tomography (PET/CT) with anti-1-amino-3-[18F]-flurocyclobutane-1-carboxylic acid (18F-FACBC) in patients with biochemical recurrence (BCR) from prostate cancer (PC). As a secondary endpoint, we evaluated 18F-FACBC PET/CT’s impact on patients management. Clinical records of 81 patients submitted to 18F-FACBC PET/CT due to PC BCR in two Italian Nuclear Medicine Units were retrospectively assessed. DR was gauged in the whole cohort and stratifying patients by discrete intervals of PSA levels. PET/CT’s impact on clinical management was scored as (1) major if it entailed an intermodality change (e.g., from systemic to loco-regional therapy); (2) minor if it led to an intramodality change (e.g., modified radiotherapy field). PET/CT’s DR resulted in 76.9% in the whole cohort, with a positive predictive value of 96.7%. Stratified by PSA quartile intervals, PET/CT’s DR was 66.7%, 71.4%, 78.9% and 90% for PSA 0.2–0.57 ng/mL, 0.58–0.99 ng/mL, 1–1.5 ng/mL and >1.5 ng/mL without significant difference among groups (p = 0.81). The most common sites of relapse were prostate bed and pelvic lymph nodes (59.3%). PET/CT impacted on clinical management in 33/81 cases (40.7%), leading to a major change in 30 subjects (90.9%). 18F-FACBC PET/CT localized recurrence in patients with BCR, with meaningful DR also at low PSA levels and significantly impacted on clinical management.
Ribosome Biogenesis Serves as a Therapeutic Target for Treating Endometriosis and the Associated Complications
Authors: Chang Cherry Yin-Yi , Chiang An-Jen , Yan Man-Ju , Lai Ming-Tsung , Su Yun-Yi , Huang Hsin-Yi , Chang Chan-Yu , Li Ya-Hui , Li Pei-Fen , Chen Chih-Mei , Hwang Tritium , Hogg Chloe , Greaves Erin , Sheu Jim Jinn-Chyuan
Published: January 2022

Ribosome biogenesis is a cellular process critical for protein homeostasis during cell growth and multiplication. Our previous study confirmed up-regulation of ribosome biogenesis during endometriosis progression and malignant transition, thus anti-ribosome biogenesis may be effective for treating endometriosis and the associated complications. A mouse model with human endometriosis features was established and treated with three different drugs that can block ribosome biogenesis, including inhibitors against mTOR/PI3K (GSK2126458) and RNA polymerase I (CX5461 and BMH21). The average lesion numbers and disease frequencies were significantly reduced in treated mice as compared to controls treated with vehicle. Flow cytometry analyses confirmed the reduction of small peritoneal macrophage and neutrophil populations with increased large versus small macrophage ratios, suggesting inflammation suppression by drug treatments. Lesions in treated mice also showed lower nerve fiber density which can support the finding of pain-relief by behavioral studies. Our study therefore suggested ribosome biogenesis as a potential therapeutic target for treating endometriosis.
Effect of Ulinastatin on Syndecan-2-Mediated Vascular Damage in IDH2-Deficient Endothelial Cells
Authors: Choi Su-jeong , Nagar Harsha , Lee Jun Wan , Kim Seonhee , Lee Ikjun , Piao Shuyu , Jeon Byeong Hwa , Kim Cuk-Seong
Published: January 2022

Syndecan-2 (SDC2), a cell-surface heparin sulfate proteoglycan of the glycocalyx, is mainly expressed in endothelial cells. Although oxidative stress and inflammatory mediators have been shown to mediate dysfunction of the glycocalyx, little is known about their role in vascular endothelial cells. In this study, we aimed to identify the mechanism that regulates SDC2 expression in isocitrate dehydrogenase 2 (IDH2)-deficient endothelial cells, and to investigate the effect of ulinastatin (UTI) on this mechanism. We showed that knockdown of IDH2 induced SDC2 expression in human umbilical vein endothelial cells (HUVECs). Matrix metalloproteinase 7 (MMP7) influences SDC2 expression. When IDH2 was downregulated, MMP7 expression was increased, as was TGF-β signaling, which regulates MMP7. Inhibition of MMP7 activity using MMP inhibitor II significantly reduced SDC2, suggesting that IDH2 mediated SDC2 expression via MMP7. Moreover, expression of SDC2 and MMP7, as well as TGF-β signaling, increased in response to IDH2 deficiency, and treatment with UTI reversed this increase. Similarly, the increase in SDC2, MMP7, and TGF-β signaling in the aorta of IDH2 knockout mice was reversed by UTI treatment. These findings suggest that IDH2 deficiency induces SDC2 expression via TGF-β and MMP7 signaling in endothelial cells.
Penetration of the SARS-CoV-2 Spike Protein across the Blood–Brain Barrier, as Revealed by a Combination of a Human Cell Culture Model System and Optical Biosensing
Authors: Petrovszki Dániel , Walter Fruzsina R. , Vigh Judit P. , Kocsis Anna , Valkai Sándor , Deli Mária A. , Dér András
Published: January 2022

Since the outbreak of the global pandemic caused by severe acute respiratory coronavirus 2 (SARS-CoV-2), several clinical aspects of the disease have come into attention. Besides its primary route of infection through the respiratory system, SARS-CoV-2 is known to have neuroinvasive capacity, causing multiple neurological symptoms with increased neuroinflammation and blood–brain barrier (BBB) damage. The viral spike protein disseminates via circulation during infection, and when reaching the brain could possibly cross the BBB, which was demonstrated in mice. Therefore, its medical relevance is of high importance. The aim of this study was to evaluate the barrier penetration of the S1 subunit of spike protein in model systems of human organs highly exposed to the infection. For this purpose, in vitro human BBB and intestinal barrier cell–culture systems were investigated by an optical biosensing method. We found that spike protein crossed the human brain endothelial cell barrier effectively. Additionally, spike protein passage was found in a lower amount for the intestinal barrier cell layer. These observations were corroborated with parallel specific ELISAs. The findings on the BBB model could provide a further basis for studies focusing on the mechanism and consequences of spike protein penetration across the BBB to the brain.
A Comparison of Energy Consumption in American Homes by Climate Region
Authors: Debs Luciana , Metzinger Jamie
Published: January 2022

The present research analyzes the impact of nine factors related to household demographics, building equipment, and building characteristics towards a home’s total energy consumption while controlling for climate. To do this, we have surveyed single-family owned houses from the 2015 Residential Energy Consumption Survey (RECS) dataset and controlled the analysis by Building America climate zones. Our findings are based on descriptive statistics and multiple regression models, and show that for a median-sized home in three of the five climate zones, heating equipment is still the main contributor to a household’s total energy consumed, followed by home size. Social-economic factors and building age were found relevant for some regions, but often contributed less than size and heating equipment towards total energy consumption. Water heater and education were not found to be statistically relevant in any of the regions. Finally, solar power was only found to be a significant factor in one of the regions, positively contributing to a home’s total energy consumed. These findings are helpful for policymakers to evaluate the specificities of climate regions in their jurisdiction, especially guiding homeowners towards more energy-efficient heating equipment and home configurations, such as reduced size.
Advanced Treatment of Phosphorus Pesticide Wastewater Using an Integrated Process of Coagulation and Ozone Catalytic Oxidation
Authors: Cao Shengping , Chen Lei , Zhao Minyan , Liu Ankang , Wang Mingxiu , Sun Yongjun
Published: January 2022

Conventional pretreatment and secondary biochemical treatment are ineffective methods for removing phosphorus from phosphorus-containing pesticide wastewater. In this study, coagulation-coupled ozone catalytic oxidation was used to treat secondary biochemical tailwater of phosphorus-containing pesticide wastewater thoroughly. The effects of the coagulant type, coagulant dosage, coagulant concentration, wastewater pH, stirring rate, and stirring time on the removal efficiency of chemical oxygen demand (COD), total phosphorus (TP), and chromaticity were investigated during coagulation. When the dosage of the coagulant PAFS was equal to 100 mg/L, the concentration of the coagulant, pH, stirring rate, and stirring time were 5 wt%, 8, 100 rpm, and 5 min, respectively, and the removal rates of COD, TP, and chroma in wastewater reached the maximum value of 17.6%, 86.8%, and 50.0%, respectively. Effluent after coagulation was treated via ozone catalytic oxidation. When the respective ozone dosage, H2O2 dosage, catalyst dosage, and reaction time were 120 mg/L, 0.1 vt‰, 10 wt%, and 90 min, residual COD and chromaticity of the final effluent were 10.3 mg/L and 8, respectively. The coagulation-coupled ozone catalytic oxidation process has good application prospects in the treatment of secondary biochemical tailwater from phosphorus-containing pesticide wastewater.
Synthesis of Indoles by Palladium-Catalyzed Reductive Cyclization of β-Nitrostyrenes with Phenyl Formate as a CO Surrogate
Authors: Ferretti Francesco , Fouad Manar Ahmed , Ragaini Fabio
Published: January 2022

The reductive cyclization of suitably substituted organic nitro compounds by carbon monoxide is a very appealing technique for the synthesis of heterocycles because of its atom efficiency and easiness of separation of the only stoichiometric byproduct CO2, but the need for pressurized CO has hampered its diffusion. We have recently reported on the synthesis of indoles by reductive cyclization of o-nitrostyrenes using phenyl formate as a CO surrogate, using a palladium/1,10-phenanthroline complex as catalyst. However, depending on the desired substituents on the structure, the use of β-nitrostyrenes as alternative reagents may be advantageous. We report here the results of our study on the possibility to use phenyl formate as a CO surrogate in the synthesis of indoles by reductive cyclization of β-nitrostyrenes, using PdCl2(CH3CN)2 + phenanthroline as the catalyst. It turned out that good results can be obtained when the starting nitrostyrene bears an aryl substituent in the alpha position. However, when no such substituent is present, only fair yield of indole can be obtained because the base required to decompose the formate also catalyzes an oligo-polymerization of the starting styrene. The reaction can be performed in a single glass pressure tube, a cheap and easily available piece of equipment.
The Longevity-Associated Variant of BPIFB4 Reduces Senescence in Glioma Cells and in Patients’ Lymphocytes Favoring Chemotherapy Efficacy
Authors: Puca Annibale Alessandro , Lopardo Valentina , Montella Francesco , Di Pietro Paola , Cesselli Daniela , Rolle Irene Giulia , Bulfoni Michela , Di Sarno Veronica , Iaconetta Giorgio , Campiglia Pietro , Vecchione Carmine , Beltrami Antonio Paolo , Ciaglia Elena
Published: January 2022

Glioblastoma (GBM) is the most common primary brain cancer with the median age at diagnosis around 64 years, thus pointing to aging as an important risk factor. Indeed, aging, by increasing the senescence burden, is configured as a negative prognostic factor for GBM stage. Furthermore, several anti-GBM therapies exist, such as temozolomide (TMZ) and etoposide (ETP), that unfortunately trigger senescence and the secretion of proinflammatory senescence-associated secretory phenotype (SASP) factors that are responsible for the improper burst of (i) tumorigenesis, (ii) cancer metastasis, (iii) immunosuppression, and (iv) tissue dysfunction. Thus, adjuvant therapies that limit senescence are urgently needed. The longevity-associated variant (LAV) of the bactericidal/permeability-increasing fold-containing family B member 4 (BPIFB4) gene previously demonstrated a modulatory activity in restoring age-related immune dysfunction and in balancing the low-grade inflammatory status of elderly people. Based on the above findings, we tested LAV-BPIFB4 senotherapeutic effects on senescent glioblastoma U87-MG cells and on T cells from GBM patients. We interrogated SA-β-gal and HLA-E senescence markers, SASP factors, and proliferation and apoptosis assays. The results highlighted a LAV-BPIFB4 remodeling of the senescent phenotype of GBM cells, enhancement of their sensitivity to temozolomide and a selective reduction of the T cells’ senescence from GBM patients. Overall, these findings candidate LAV-BPIFB4 as an adjuvant therapy for GBM.
Application of Aminopolycarboxylic Complexes of V(IV) in Catalytic Adsorptive Stripping Voltammetry of Germanium
Authors: Królicka Agnieszka , Zarębski Jerzy , Bobrowski Andrzej
Published: January 2022

In the review, voltammetric analytical procedures that employ vanadium(IV) and aminopolycarboxylic complexes of V(IV) are presented and discussed. The focus of the paper is on the mechanism of vanadium-catalyzed reactions responsible for the amplification of the analytical signal of Ge(IV). The analytical efficacy of different catalytic systems is compared, and the optimal parameters of the respective procedures are reported.
The Association between Participation in Fights and Bullying and the Perception of School, Teachers, and Peers among School-Age Children in Serbia
Authors: Stankovic Sanja , Santric-Milicevic Milena , Nikolic Dejan , Bjelica Nenad , Babic Uros , Rakic Ljiljana , Terzic-Supic Zorica , Todorovic Jovana
Published: January 2022

Participating in physical fighting and bullying can be a cause of severe injury and death among school-age children. Research evidence can support school and health actors’ efforts to improve school-age children’s development and health capacity for life. The study aims to assess the prevalence of school-age children’s participation in fights and bullying in Serbia, and to examine the relevance of students’ socio-demographic characteristics and perceptions of school and relations with other students and professors for participation in fights and bullying. A secondary analysis is also performed on the original data of the 2017 HBSC study, which was conducted on 3267 students in a nationally representative sample of primary and high schools in Serbia. We sought to investigate the relationship between eight socio-demographic characteristics and nine school-related perceptions, with two outcome variables: taking part in fights and taking part in bullying, examined by using univariate and multivariate logistic regression. The main results show that 50.8% of boys and 17.1% of girls have taken part in fights, while 17.7% boys and 10.4% of girls have taken part in bullying. Students who felt a large and very large burden of school obligations were 1.43 times more likely to participate in bullying at least once, while they were 1.38 and 2.12 times more likely to participate in multiple fights and 4.04, 1.24, and 2.78 times more likely to participate multiple times in bullying. Multiple participation in fights and in bullying is significantly negatively associated with female gender, younger age years, good and very good perception of family financial status and quality of life, and positive perceptions of school and relations they have with other students and professors. Fights among school-age children are significantly positively associated with living with relatives/legal guardians and poor quality of life. In conclusion, the prevalence of participating in at least one fight/bullying is higher than in multiple fights/bullying. These associations suggest a necessity to enhance the monitoring and control of peer behavior among school-age children. The findings of the study imply key enablers of protection, such as building relationships based on team spirit and work, friendly behavior, empathy, and help, which should be included in the value system of school and family activities in programs to combat fights and bullying in school-age children.
Computer-Assisted Discovery of Alkaloids with Schistosomicidal Activity
Authors: Menezes Renata Priscila Barros de , Viana Jéssika de Oliveira , Muratov Eugene , Scotti Luciana , Scotti Marcus Tullius
Published: January 2022

Schistosomiasis is a chronic parasitic disease caused by trematodes of the genus Schistosoma; it is commonly caused by Schistosoma mansoni, which is transmitted by Bioamphalaria snails. Studies show that more than 200 million people are infected and that more than 90% of them live in Africa. Treatment with praziquantel has the best cost–benefit result on the market. However, hypersensitivity, allergy, and drug resistance are frequently presented after administration. From this perspective, ligand-based and structure-based virtual screening (VS) techniques were combined to select potentially active alkaloids against S. mansoni from an internal dataset (SistematX). A set of molecules with known activity against S. mansoni was selected from the ChEMBL database to create two different models with accuracy greater than 84%, enabling ligand-based VS of the alkaloid bank. Subsequently, structure-based VS was performed through molecular docking using four targets of the parasite. Finally, five consensus hits (i.e., five alkaloids with schistosomicidal potential), were selected. In addition, in silico evaluations of the metabolism, toxicity, and drug-like profile of these five selected alkaloids were carried out. Two of them, namely, 11,12-methylethylenedioxypropoxy and methyl-3-oxo-12-methoxy-n(1)-decarbomethoxy-14,15-didehydrochanofruticosinate, had plausible toxicity, metabolomics, and toxicity profiles. These two alkaloids could serve as starting points for the development of new schistosomicidal compounds based on natural products.
Physiological, Pathological and Pharmacological Interactions of Hydrogen Sulphide and Nitric Oxide in the Myocardium of Rats with Left Ventricular Hypertrophy
Authors: Ahmad Ashfaq
Published: January 2022

Left ventricular hypertrophy (LVH) is characterized by increased myocardium thickness due to increased oxidative stress and downregulation of cystathione γ lyase (CSE) endothelial nitric oxide synthase (eNOS). Upregulation of CSE by hydrogen sulphide (H2S) and ENOS by L-arginine can arrest the progression of LVH individually. The present study explored the combined treatment of H2S and NO in the progression of LVH, and demonstrated that the response is due to H2S, NO or formation of either new molecule in physiological, pathological, and pharmacological in vivo settings of LVH. Exogenous administration H2S+NO in LVH significantly reduced (all p < 0.05) systolic blood pressure (SBP) and mean arterial pressure (MAP), LV index, heart index and oxidative stress when compared to the LVH group. There was downregulation of CSE mRNA and eNOS in the heart, and exogenous administration of H2S+NO groups upregulated eNOS MRNA while CSE MRNA remained downregulated in the hearts of the LVH group. Similar trends were observed with concentrations of H2S and NO in the plasma and tissue. It can be concluded that combined treatment of LVH with H2S and NO significantly ameliorate the progression of LVH by attenuating systemic hemodynamic and physical indices, and by decreasing oxidative stress. Molecular expression data in the myocardium of LVH depicts that combined treatment upregulated eNOS/NO while it downregulated CSE/H2S pathways in in vivo settings, and it is always eNOS/NO pathways which play a major role.