bluuebunny/crossref_metadata_2025 huggingface dataset:
Perception and Awareness about Developmental Dysplasia of the Hip in Children among Pregnant Ladies in the Aseer Region, Southwestern Saudi Arabia
Authors: Alqarni Mahdi M. , Shati Ayed A. , Al-Qahtani Youssef A. , Alhifzi Wafaa S. , Alhifzi Wael S. , Al Saleh Rasha S. , Alqahtani Nada A. , Alshehri Mohammed A.
Published: October 2021

Background: Developmental dysplasia of the hip (DDH) is classified as a group of malformations, varying from abnormal acetabulum (dysplasia) and mild subluxation of the femoral head to fixed displacement (congenital dislocation). This study aimed to assess the knowledge level and its determinants regarding DDH in children among pregnant females in the Aseer region of southwestern Saudi Arabia. Methods: A descriptive cross-sectional study was conducted targeting all pregnant females in the Aseer region between 1 February 2021 and 1 May 2021. A pre-structured online questionnaire was constructed by the researchers to obtain the participating females’ bio-demographic data (including age, education status, and obstetric history) and awareness regarding DDH. The last section asked for their source of information regarding DDH. Results: A total of 253 pregnant females (aged between 18 and 45 years; mean age = 30.5 ± 10.2 years) fulfilling the inclusion criteria completed the study questionnaire. About 5% of the females reported having a child with DDH, and 166 (65.6%) pregnant females knew about DDH. Additionally, 110 (43.5%) females reported that they know about how DDH is treated, and 99 (39.1%) knew about DDH complications. The most commonly reported source of information was relatives and friends (44.3%), followed by social media (11.9%) and study and work (7.1%). Conclusions: Pregnant females in the Aseer region have poor knowledge and awareness about DDH and its causes, treatment modalities, and complications. Higher knowledge was associated with either high parity or having a child with DDH.
Trends and Factors Associated with Oral Contraceptive Use among Korean Women
Authors: Park Hyejin , Kim Kisok
Published: October 2021

Although oral contraceptives (OC) are widely used, few national-level epidemiologic studies have evaluated the prevalence of OC use and factors related to their use in Korea. We performed a population-based cross-sectional study on OC use by premenopausal women aged 20–59 years residing in Korea. We used secondary data from the 2010–2019 National Health and Nutrition Examination Survey to examine trends in the annual prevalence of OC use between 2010 and 2019, and factors influencing OC use. Based on data from 14,386 premenopausal women, the average annual prevalence of OC use was 8.2–10.7% between 2010 and 2014; it increased to 12.6–14.4% during 2015–2019. The prevalence of OC use was significantly higher in women with higher (≥5) than lower gravidity (<5). In addition, among sociodemographic factors, education level, household income, cigarette smoking, and alcohol drinking were significantly associated with OC use in Korean women. As OC use is affected by sociodemographic factors, a contraceptive plan that considers sociodemographic factors is needed to establish an effective family planning policy.
Current Novel Caries Diagnostic Technologies: Restorative Dentists’ Attitude and Use Preferences
Authors: Nassar Hani M. , Yeslam Hanin E.
Published: October 2021

Early detection of caries lesions is key to a successful restorative dental treatment plan. The aim of this study was to investigate the preferences and attitude of graduate restorative dentistry residents (RDRs) regarding novel caries diagnostic technologies (NCDT) and to provide a brief overview of available technologies for both specialized and general dental practice. This cross-sectional study used an online questionnaire (17 questions) concerning RDRs’ attitude, preferences, and insights regarding five available NCDTs. It was distributed among twenty RDRs at a local government dental school following a review session about NCDTs. Collected responses were analyzed statistically using one-way analysis of variance (ANOVA), chi-squared with Bonferroni correction, and Kruskal-Wallis tests at a 0.05 significance level. Sixty-five percent of RDRs reported an interest in NCDTs as a discussion topic and almost half of them were positive towards their use, however, sixty percent of respondents were hesitant to diagnose caries solely using NCDTs. Fiber-optic-transillumination (FOTI) systems were ranked the best overall and with regard to all the investigated criteria (p < 0.05). Chosen reasons for FOTI included price followed by ease of use. In general, high price rated as the most perceived reason for not choosing a given NCDT followed by low practical applicability. Meanwhile, ease of use followed by relevant application ranked as the main reported reasons to choose an NCDTs.
Mechanisms of Nitric Oxide in the Regulation of Chilling Stress Tolerance in Camellia sinensis
Authors: Wang Yingzi , Yu Qin , Li Yinhua , Li Juan , Chen Jinhua , Liu Zhonghua , Huang Jian’an , Al-Harbi Mohammad S. , Ali Esmat F. , Eissa Mamdouh A.
Published: October 2021

Tea [Camellia sinensis (L.)] plants are important economic crop in China. Chilling stress and freezing damages have seriously affected the quality of tea products that have been already regarded as the main restricting factors to industry’s development. Nitric oxide (NO) plays a crucial role in resistance of abiotic stresses. An experiment was conducted in an artificial climate chamber to study the effect of NO on tea plants grown under chilling stress (−2 °C) for 0, 6, 24, 48, and 72 h. Foliar application of sodium nitroprusside (SNP) at a rate of 500 μmol·L−1 was used as NO donor. The experiment contained two factors: the first was the foliar application with SNP or distilled water, and the scond one was the chilling (−2 °C) exposure time (0, 6, 24, 48, and 72 h). The effects of NO on membrane lipid peroxidation, osmotic adjustment substances, and antioxidant activity under cold stress were studied. In addition, the gene expression of CsICE1 and CsCBF1 in respond to NO addition were also investigated using real-time polymerase chain reaction (RT-PCR). The results show that foliar addition of NO (500 μmol·L−1 of SNP) reduce the relative conductivity of tea leaves, inhibits the elevated malondialdehyde content, promotes the accumulation of proline, soluble protein and sugar, and increases the superoxide dismutase, catalase activities, thereby alleviates the damage of cold stress on tea leaves. The CsICE1 expression in 500 μM SNP treatment was peaked at 24 h of low temperature stress, while it did not express at normal temperature. Therefore, the current study is considered a good scientific material in understanding how tea plants sense and defense the chilling stress and that plays an important role to improve the level of production and economic benefits. It is also provided significant theory bas to control chilling stress in tea plants.
Investigation and Evaluation of Impact Bruising in Guava Using Image Processing and Response Surface Methodology
Authors: Htike Than , Saengrayap Rattapon , Aunsri Nattapol , Tontiwattanakul Khemapat , Chaiwong Saowapa
Published: October 2021

Simulated impact damage testing was investigated by fractal image analysis using response surface methodology (RSM) with a central composite design (CCF) on quality of ‘Glom Sali’ guava for drop heights (0.2, 0.4, and 0.6 m), number of drops (1, 3, and 5) and storage temperature conditions (10, 20, and 30 °C). After 48 h, impacted fruit were determined and analyzed for bruise area (BA), bruise volume (BV), browning index (BI), total color difference (∆E), image analysis for bruise area (BAI), and fractal dimension (FD) at the bruising region on peeled guava. Results showed that the correlation coefficient (r = −0.6055) between ∆E and FD value was higher than ∆E and either BA (r = 0.3132) or BV (r = 0.2095). The FD variable was determined as a better indicator than conventional measurement (BA or BV) for pulp browning and impact bruising susceptibility. The FD variable also exhibited highest R2adj value (81.69%) among the other five variables, as the highest precision model with high determination coefficient value (R2adj) (>0.8) for impact bruising prediction. Recommended condition of the FD variable to minimize impact bruising was drop height of 0.53 m for five drops under storage at 30 °C. FD variable assessed by image analysis was shown to be a highly capable measurement to determine impact bruising susceptibility in guava fruit.
Critical Factors for Predicting Users’ Acceptance of Digital Museums for Experience-Influenced Environments
Authors: Wu Yue , Jiang Qianling , Ni Shiyu , Liang Hui’e
Published: October 2021

Digital museums that use modern technology are gradually replacing traditional museums to stimulate personal growth and promote cultural exchange and social enrichment. With the development and popularization of the mobile Internet, user experience has become a concern in this field. From the perspective of the dynamic stage of user experience, in this study, we expand ECM and TAM by combining the characteristics of users and systems, thereby, constructing the theoretical model and 12 hypotheses about the influencing factors of users’ continuance intentions toward digital museums. A total of 262 valid questionnaires were collected, and the structural equation model tested the model. This study identifies variables that play a role and influence online behavior in a specific experiential environment: (1) Perceived playfulness, perceived usefulness, and satisfaction are the critical variables that affect users’ continuance intentions. (2) Expectation confirmation has a significant influence on perceived playfulness, perceived ease of use, and satisfaction. (3) Media richness is an essential driver of confirmation, perceived ease of use, and perceived usefulness. The conclusions can be used as a reference for managers to promote the construction and innovation of digital museums and provide a better experience to meet users’ needs.
Nanostructure and Luminescent Properties of Bimetallic Lanthanide Eu/Gd, Tb/Gd and Eu/Tb Coordination Polymers
Authors: Brunckova Helena , Mudra Erika , Rocha Lucas , Nassar Eduardo , Nascimento Willian , Kolev Hristo , Lisnichuk Maksym , Kovalcikova Alexandra , Molcanova Zuzana , Strečkova Magdalena , Medvecky Lubomir
Published: October 2021

This study presents the synthesis, structural and luminescence properties for lanthanide metal–organic frameworks (LnMOFs), which belong to the sub-class of coordination polymers. The series of nanosized LnMOFs (Ln = Eu, Gd, Tb, Eu0.5Gd0.5, Tb0.5Gd0.5 and Eu0.5Tb0.5) was prepared by solvothermal synthesis using a modulator (sodium acetate). We investigated the various surface chemistry compositions of the isostructural LnMOFs with a [Ln(btc)] structure (BTC: Benzene-1,3,5-tricarboxylate) by X-ray photoelectron spectroscopy (XPS). The XPS confirmed the mixed-valent Eu3+ and Eu2+ compounds, and the presence of Tb in both +3 and +4 valence states, and one +3 valency of Gd. A nanostructure of mixed LnMOFs (EuGd, TbGd and EuTb) with a rod-like shape is related to luminescence properties. The MOFs (EuTb and EuGd) presented Comission Internationale de l’Éclairage (CIE) chromaticities of x = 0.666 and y = 0.331, and x = 0.654 and y = 0.348, respectively, in the red region. They were better than the values desired for use in commercial phosphors, which are x = 0.64 and y = 0.35. For [Tb/Gd(btc)], the CIE coordinates were x = 0.334 and y = 0.562, presenting emissions in the green region. Bimetallic LnMOFs are very promising UV light sensors for biological applications.
Exploring the Level of Post Traumatic Growth in Kidney Transplant Recipients via Network Analysis
Authors: Battaglia Yuri , Zerbinati Luigi , Belvederi Murri Martino , Provenzano Michele , Esposito Pasquale , Andreucci Michele , Storari Alda , Grassi Luigi
Published: October 2021

Although kidney transplant can lead to psychiatric disorders, psychosocial syndromes and demoralization, a positive post-traumatic growth (PTG) can occur in kidney transplant recipients (KTRs). However, the PTG-Inventory (PTGI), a reliable tool to measure PTG is scarcely used to explore the effect of this stressful event in KTRs. Thus, the purpose of our study was to assess the level of PTG and its correlation with demoralization, physical and emotional symptoms or problems via network analysis in KTRs. Additionally, we aimed at exploring the association of PTG with psychiatric diagnoses, Diagnostic Criteria for Psychosomatic Research (DCPR) conditions, and medical variables. A total of 134 KTRs were tested using MINI International Neuropsychiatric Interview 6.0 (MINI 6.0), DCPR interview, PTGI, Edmonton Symptom Assessment System (ESAS), Canadian Problem Checklist (CPC) and Demoralization scale (DS-IT). PTGI was used to investigate the positive psychological experience of patients after KT. It consists of 21 items divided in five factors. Routine biochemistry, immunosuppressive agents, socio-demographic and clinical data were collected. A symptom network analysis was conducted among PTGI, ESAS and DS-IT. Mean score of PTGI total of sample was 52.81 ± 19.81 with higher scores in women (58.53 ± 21.57) than in men (50.04 ± 18.39) (p < 0.05). PTGI-Relating to Others (16.50 ± 7.99) sub-score was markedly higher than other PTGI factor sub-scores. KTRs with DCPR-alexithymia or International Classification of Diseases, tenth revision (ICD-10) anxiety disorders diagnosis had lower PTGI total score and higher PTGI-Personal Strength sub-score, respectively (p < 0.05). The network analysis identified two communities: PTGI and ESAS with DS-IT. DS-IT Disheartenment, DS-IT Hopelessness and PTGI Relating to Others were the most central items in the network. After 1000 bootstrap procedures, the Exploratory graph analysis revealed the presence of a median of two communities in the network in 97.5% of the bootstrap iterations. A more extensive use of PTGI should be encouraged to identify and enhance the positive psychological changes after KT.
Recurrence of Hepatic Encephalopathy after TIPS: Effective Prophylaxis with Combination of Lactulose and Rifaximin
Authors: Seifert Leon Louis , Schindler Philipp , Schoster Martin , Weller Jan Frederic , Wilms Christian , Schmidt Hartmut H. , Maschmeier Miriam , Masthoff Max , Köhler Michael , Heinzow Hauke , Wildgruber Moritz
Published: October 2021

Background: Transjugular intrahepatic portosystemic shunt (TIPS) implantation is an established procedure to treat portal hypertension with hepatic encephalopathy (HE) as a common complication. There is lack of evidence concerning HE prophylaxis after TIPS. Methods: N = 233 patients receiving TIPS between 2011 and 2018 at a German tertiary care center were included. Of them, 21% (n = 49) had a history of HE. The follow-up period was 12 months. The risk factors of post-TIPS HE were analyzed via multivariate analysis. The efficacy of prophylactic medication regimens was studied. The results show that 35.6% (n = 83) received no medication (NM), 36.5% (n = 85) received lactulose monoprophylaxis (LM), 2.6% (n = 6) rifaximin monoprophylaxis (RM) and 25.3% (n = 59) lactulose and rifaximin (LR) of which 64.4% received l-ornithin-l-aspartate (LOLA) additionally (LR + LOLA) and 36.6% did not (LRonly). Results: Multivariate analysis revealed higher age (p = 0.003) and HE episodes prior to TIPS (p = 0.004) as risk factors for HE after TIPS. LM has no prophylactic effect. LR prevents HE recurrence at 1, 3 and 12 months after TIPS (p = 0.003, p = 0.003, p = 0.006) but does not prevent HE in patients with no history of HE (p = 0.234, p = 0.483, p = 0.121). LR prevents HE recurrence compared with LM/NM (25.0% vs. 64.7%, p = 0.007) within 12 months after TIPS, whereas de novo occurrence is unaffected (p = 0.098). The additional administration of LOLA to LR has no benefit (LRonly: 25.0%, LR + LOLA: 29.7%, p = 0.780). Conclusions: Higher age and previous HE are risk factors post-TIPS HE. In patients with HE prior to TIPS, effective prophylaxis of HE is feasible via combination of lactulose and rifaximin with no additional benefit from LOLA.
Mechanically Stable β-TCP Structural Hybrid Scaffolds for Potential Bone Replacement
Authors: Ahlhelm Matthias , Latorre Sergio H. , Mayr Hermann O. , Storch Christiane , Freytag Christian , Werner David , Schwarzer-Fischer Eric , Seidenstücker Michael
Published: October 2021

The authors report on the manufacturing of mechanically stable β-tricalcium phosphate (β-TCP) structural hybrid scaffolds via the combination of additive manufacturing (CerAM VPP) and Freeze Foaming for engineering a potential bone replacement. In the first step, load bearing support structures were designed via FE simulation and 3D printed by CerAM VPP. In the second step, structures were foamed-in with a porous and degradable calcium phosphate (CaP) ceramic that mimics porous spongiosa. For this purpose, Fraunhofer IKTS used a process known as Freeze Foaming, which allows the foaming of any powdery material and the foaming-in into near-net-shape structures. Using a joint heat treatment, both structural components fused to form a structural hybrid. This bone construct had a 25-fold increased compressive strength compared to the pure CaP Freeze Foam and excellent biocompatibility with human osteoblastic MG-63 cells when compared to a bone grafting Curasan material for benchmark.
Towards the Determination of Machining Allowances and Surface Roughness of 3D-Printed Parts Subjected to Abrasive Flow Machining
Authors: Samoilenko Mykhailo , Lanik Greg , Brailovski Vladimir
Published: October 2021

Abrasive flow machining (AFM) is considered as one of the best-suited techniques for surface finishing of laser powder bed fused (LPBF) parts. In order to determine the AFM-related allowances to be applied during the design of LPBF parts, a numerical tool allowing to predict the material removal and the surface roughness of these parts as a function of the AFM conditions is developed. This numerical tool is based on the use of a simplified viscoelastic non-Newtonian medium flow model and calibrated using specially designed artifacts containing four planar surfaces with different surface roughnesses to account for the build orientation dependence of the surface finish of LPBF parts. The model calibration allows the determination of the abrasive medium-polished part slip coefficient, the fluid relaxation time and the abrading (Preston) coefficient, as well as of the surface roughness evolution as a function of the material removal. For model validation, LPBF parts printed from the same material as the calibration artifacts, but having a relatively complex tubular geometry, were polished using the same abrasive medium. The average discrepancy between the calculated and experimental material removal and surface roughness values did not exceed 25%, which is deemed acceptable for real-case applications. A practical application of the numerical tool developed was demonstrated using the predicted AFM allowances for the generation of a compensated computer-aided design (CAD) model of the part to be printed.
Facies Analysis and Sedimentary Architecture of Hybrid Event Beds in Submarine Lobes: Insights from the Crocker Fan, NW Borneo, Malaysia
Authors: Jamil Muhammad , Siddiqui Numair Ahmed , Ahmed Nisar , Usman Muhammad , Umar Muhammad , Rahim Hamad ur , Imran Qazi Sohail
Published: October 2021

Hybrid event beds represent the combined effect of multiple geological processes, which result in complex depositional geometries and distinct facies distribution in marine environments. Previous work on hybrid event beds highlights the classification, origin, and types of hybrid facies. However, in the present study, we discuss the development of hybrid event beds in submarine lobes with an emphasis on the analysis of proximal to distal, frontal to lateral relationships and evolution during lobe progradation. Detailed geological fieldwork was carried out in the classical deep-marine Late Paleogene Crocker Fan to understand the relationship between the character of hybrid bed facies and lobe architecture. The results indicate that hybrid facies of massive or structureless sandstone with mud clasts, clean to muddy sand, and chaotic muddy sand with oversized sand patch alternations (H1–H3) are well developed in proximal to medial lobes, while distal lobes mainly contain parallel to cross-laminated clean to muddy hybrid facies (H3–H5). Furthermore, lateral lobes have less vertical thickness of hybrid beds than frontal lobes. The development of hybrid beds takes place in the lower part of the thickening upward sequence of lobe progradation, while lobe retrogradation contains hybrid facies intervals in the upper part of stratigraphy. Hence, the development of hybrid beds in submarine lobe systems has a significant impact on the characterization of heterogeneities in deep-marine petroleum reservoirs at sub-seismic levels.
Geometry Optimization of Heaving Axisymmetric Point Absorbers under Parametrical Constraints in Irregular Waves
Authors: Wu Jinming
Published: October 2021

The objective of this work is to identify the maximum absorbed power and optimal buoy geometry of a heaving axisymmetric point absorber for a given cost in different sea states. The cost of the wave energy converter is estimated as proportional to the displaced volume of the buoy, and the buoy geometry is described by the radius-to-draft ratio. A conservative wave-height-dependent motion constraint is introduced to prevent the buoy from jumping out of the free surface of waves. The constrained optimization problem is solved by a two-nested-loops method, within which a core fundamental optimization process employs the MATLAB function fmincon. Results show that the pretension of the mooring system should be as low as possible. Except for very small energy periods, the stiffness of both the power take-off and mooring system should also be as low as possible. A buoy with a small radius-to-draft ratio can absorb more power, but at the price of working in more energetic seas and oscillating at larger amplitudes. In addition, the method to choose the optimal buoy geometry at different sea states is provided.
Effects of Wavy Leading-Edge Protuberance on Hydrofoil Performance and Its Flow Mechanism
Authors: Li Jing , Liu Chunbao , Li Xiaoying
Published: October 2021

This paper examines the effects on a Clark-y three-dimensional hydrofoil of wavy leading-edge protuberances in a quantitative and qualitative way. The simulation is accompanied by a hybrid RANS-LES model in conjunction with Zwart-Gerber–Belamri model. Detailed discussions of the stable no-cavitating, unsteady cavitating flow fields and the control mechanics are involved. The force characteristics, complicated flow behaviors, cavitation–streamwise vortex interactions, and the cavitating flow instability are all presented. The results demonstrate that protuberances acting as vortex generators produce a continuous influx of boundary-layer vorticity, significantly enhancing the momentum transfer of streamwise vortices and therefore improving the hydrodynamics of the hydrofoil. Significant interactions are described, including the encouragement impact of cavitation evolution on the fragmentation of streamwise vorticities as well as the compartmentation effect of streamwise vorticities binding the cavitation inception inside the troughs. The variations in cavitation pressure are mainly due to the acceleration in steam volume. In summary, it is vital for new hydrofoils or propeller designs to understand in depth the effects of leading-edge protuberances on flow control.
Propagation Properties of an Off-Axis Hollow Gaussian-Schell Model Vortex Beam in Anisotropic Oceanic Turbulence
Authors: Wang Xinguang , Wang Le , Zhao Shengmei
Published: October 2021

Based on the extended Huygens–Fresnel principle and the power spectrum of anisotropic oceanic turbulence, the analytical expressions of the average intensity and coherence properties of an off-axis hollow Gaussian-Schell model (OAHGSM) vortex beam propagating through anisotropic oceanic turbulence were derived. The effects of turbulent ocean and beam characteristic parameters on the evolution properties of the OAHGSM vortex beam were analyzed in detail. Our numerical simulation results showed that the OAHGSM vortex beam with a larger position factor is more focusable. Meanwhile, the OAHGSM vortex beam eventually evolves into a Gaussian-like beam after propagating through the anisotropic oceanic turbulent channel. The speed of this process can be accelerated by the decrease of the hollow order, topological charge, beam width, and transverse coherence width of the beam. The results also indicated that the normalized average intensity spreads more greatly and the spectral degree of coherence decays more rapidly for the smaller dissipation rate of the kinetic energy per unit mass of fluid, the smaller anisotropic coefficient, the smaller inner scale factor, the larger dissipation rate of the mean-squared temperature, and the higher temperature–salinity contribution ratio.
Cellulase Enzyme Production from Filamentous Fungi Trichoderma reesei and Aspergillus awamori in Submerged Fermentation with Rice Straw
Authors: Naher Laila , Fatin Siti Noor , Sheikh Md Abdul Halim , Azeez Lateef Adebola , Siddiquee Shaiquzzaman , Zain Norhafizah Md , Karim Sarker Mohammad Rezaul
Published: October 2021

Fungi are a diverse group of microorganisms that play many roles in human livelihoods. However, the isolation of potential fungal species is the key factor to their utilization in different sectors, including the enzyme industry. Hence, in this study, we used two different fungal repositories—soil and weed leaves—to isolate filamentous fungi and evaluate their potential to produce the cellulase enzyme. The fungal strains were isolated using dichloran rose bengal agar (DRBA) and potato dextrose agar (PDA). For cellulase enzyme production, a rice straw submerged fermentation process was used. The enzyme production was carried out at the different incubation times of 3, 5, and 7 days of culture in submerged conditions with rice straw. Fungal identification studies by morphological and molecular methods showed that the soil colonies matched with Trichoderma reesei, and the weed leaf colonies matched with Aspergillus awamori. These species were coded as T. reesei UMK04 and A. awamori UMK02, respectively. This is the first report of A. awamori UMK02 isolation in Malaysian agriculture. The results of cellulase production using the two fungi incorporated with rice straw submerged fermentation showed that T. reesei produced a higher amount of cellulase at Day 5 (27.04 U/mg of dry weight) as compared with A. awamori (15.19 U/mg of dry weight), and the concentration was significantly different (p < 0.05). Our results imply that T. reesei can be utilized for cellulase production using rice straw.
Regulation of Pkc1 Hyper-Phosphorylation by Genotoxic Stress
Authors: Liu Li , Veis Jiri , Reiter Wolfgang , Motari Edwin , Costello Catherine E. , Samuelson John C. , Ammerer Gustav , Levin David E.
Published: October 2021

The cell wall integrity (CWI) signaling pathway is best known for its roles in cell wall biogenesis. However, it is also thought to participate in the response to genotoxic stress. The stress-activated protein kinase Mpk1 (Slt2, is activated by DNA damaging agents through an intracellular mechanism that does not involve the activation of upstream components of the CWI pathway. Additional observations suggest that protein kinase C (Pkc1), the top kinase in the CWI signaling cascade, also has a role in the response to genotoxic stress that is independent of its recognized function in the activation of Mpk1. Pkc1 undergoes hyper-phosphorylation specifically in response to genotoxic stress; we have found that this requires the DNA damage checkpoint kinases Mec1 (Mitosis Entry Checkpoint) and Tel1 (TELomere maintenance), but not their effector kinases. We demonstrate that the casein kinase 1 (CK1) ortholog, Hrr25 (HO and Radiation Repair), previously implicated in the DNA damage transcriptional response, associates with Pkc1 under conditions of genotoxic stress. We also found that the induced association of Hrr25 with Pkc1 requires Mec1 and Tel1, and that Hrr25 catalytic activity is required for Pkc1-hyperphosphorylation, thereby delineating a pathway from the checkpoint kinases to Pkc1. We used SILAC mass spectrometry to identify three residues within Pkc1 the phosphorylation of which was stimulated by genotoxic stress. We mutated these residues as well as a collection of 13 phosphorylation sites within the regulatory domain of Pkc1 that fit the consensus for CK1 sites. Mutation of the 13 Pkc1 phosphorylation sites blocked hyper-phosphorylation and diminished RNR3 (RiboNucleotide Reductase) basal expression and induction by genotoxic stress, suggesting that Pkc1 plays a role in the DNA damage transcriptional response.
A Mathematical Model of Breast Tumor Progression Based on Immune Infiltration
Authors: Mohammad Mirzaei Navid , Su Sumeyye , Sofia Dilruba , Hegarty Maura , Abdel-Rahman Mohamed H. , Asadpoure Alireza , Cebulla Colleen M. , Chang Young Hwan , Hao Wenrui , Jackson Pamela R. , Lee Adrian V. , Stover Daniel G. , Tatarova Zuzana , Zervantonakis Ioannis K. , Shahriyari Leili
Published: October 2021

Breast cancer is the most prominent type of cancer among women. Understanding the microenvironment of breast cancer and the interactions between cells and cytokines will lead to better treatment approaches for patients. In this study, we developed a data-driven mathematical model to investigate the dynamics of key cells and cytokines involved in breast cancer development. We used gene expression profiles of tumors to estimate the relative abundance of each immune cell and group patients based on their immune patterns. Dynamical results show the complex interplay between cells and molecules, and sensitivity analysis emphasizes the direct effects of macrophages and adipocytes on cancer cell growth. In addition, we observed the dual effect of IFN-γ on cancer proliferation, either through direct inhibition of cancer cells or by increasing the cytotoxicity of CD8+ T-cells.
Determinants of Longitudinal Change of Lung Function in Different Gender in a Large Taiwanese Population Follow-Up Study Categories: Original Investigation
Authors: Chang Chia-Heng , Chen Szu-Chia , Geng Jiun-Hung , Wu Da-Wei , Huang Jiun-Chi , Wu Pei-Yu
Published: October 2021

Chronic lung disease is associated with tremendous social and economic burden worldwide. The aim of this study was to investigate the sex-specific risk factors for changes in lung function in a large longitudinal study. We included 9059 participants from the Taiwan Biobank. None of the participants had a history of smoking, asthma, emphysema or bronchitis. Lung function was assessed using spirometry measurements of forced vital capacity (FVC) and forced expiratory volume in 1 s (FEV1). Change in the FEV1/FVC (ΔFEV1/FVC) was calculated as a follow-up FEV1/FVC minus baseline FEV1/FVC. Linear regression analysis was used to identify associations between variables and ΔFEV1/FVC in the male and female participants. After multivariable adjustments, the male participants (vs. females; p = 0.021) were significantly associated with a low ΔFEV1/FVC. In addition, the male participants with low aspartate aminotransferase (AST) (p = 0.003), high alanine aminotransferase (ALT) (p = 0.006) and a low estimated glomerular filtration rate (eGFR) (p = 0.003) were significantly associated with a low ΔFEV1/FVC. For the female participants, low systolic blood pressure (p = 0.005), low diastolic blood pressure (p = 0.031), low AST (p < 0.001), high ALT (p < 0.001) and a low eGFR (p = 0.001) were significantly associated with a low ΔFEV1/FVC. In this large follow-up study, we found that the male participants had a faster decrease in the FEV1/FVC than the female participants. In addition, liver and renal functions were correlated with changes in lung function in both the male and female participants. Our findings provide useful information on sex-specific changes in lung function.
A Clinical-Genetic Score for Predicting Weight Loss after Bariatric Surgery: The OBEGEN Study
Authors: Ciudin Andreea , Fidilio Enzamaría , Gutiérrez-Carrasquilla Liliana , Caixàs Assumpta , Vilarrasa Núria , Pellitero Silvia , Simó-Servat Andreu , Vilallonga Ramon , Ruiz Amador , de la Fuente Maricruz , Luna Alexis , Sánchez Enric , Rigla Mercedes , Hernández Cristina , Salas Eduardo , Simó Rafael , Lecube Albert
Published: October 2021

Around 30% of the patients that undergo bariatric surgery (BS) do not reach an appropriate weight loss. The OBEGEN study aimed to assess the added value of genetic testing to clinical variables in predicting weight loss after BS. A multicenter, retrospective, longitudinal, and observational study including 416 patients who underwent BS was conducted (Clinical.Trials.gov- NCT02405949). 50 single nucleotide polymorphisms (SNPs) from 39 genes were examined. Receiver Operating Characteristic (ROC) curve analysis were used to calculate sensitivity and specificity. Satisfactory response to BS was defined as at nadir excess weight loss >50%. A good predictive model of response [area under ROC of 0.845 (95% CI 0.805–0.880), p < 0.001; sensitivity 90.1%, specificity 65.5%] was obtained by combining three clinical variables (age, type of surgery, presence diabetes) and nine SNPs located in ADIPOQ, MC4R, IL6, PPARG, INSIG2, CNR1, ELOVL6, PLIN1 and BDNF genes. This predictive model showed a significant higher area under ROC than the clinical score (p = 0.0186). The OBEGEN study shows the key role of combining clinical variables with genetic testing to increase the predictability of the weight loss response after BS. This finding will permit us to implement a personalized medicine which will be associated with a more cost-effective clinical practice.
Sovereign Default Forecasting in the Era of the COVID-19 Crisis
Authors: Kristóf Tamás
Published: October 2021

The COVID-19 crisis has revealed the economic vulnerability of various countries and, thus, has instigated the systematic exploration and forecasting of sovereign default risks. Multivariate statistical and stochastic process-based sovereign default risk forecasting has a 50-year developmental history. This article describes a continuous, non-homogeneous Markov chain method as the basis for a COVID-19-related sovereign default risk forecast model. It demonstrates the estimation of sovereign probabilities of default (PDs) over a five-year horizon period with the developed model reflecting the impact of the COVID-19 crisis. The COVID-19-adopted Markov model estimates PDs for most countries, including those that are advanced with AAA and AA ratings, to suggest that no sovereign nation’s economy is secure from the financial impact of the COVID-19 pandemic. The dynamics of the estimated PDs are indicative of contemporary evidence as experienced in the recent financial crisis. The empirical results of this article have policy implications for foreign investors, sovereign lenders, export finance institutions, foreign trade experts, risk management professionals, and policymakers in the field of finance. The developed model can be used to timely recognize potential problems with sovereign entities in the current COVID-19 crisis and to take appropriate mitigating actions.
Royal Land Use and Management in Beijing in the Qing Dynasty
Authors: Xiao Yao , Liu Lian
Published: October 2021

Management is an important factor affecting the formation and development of a landscape. This study concludes that royal land use is planned by specifying the land type, while the economic benefits and landscape value are also taken into account. The royal land has landscape value, with the core being the royal garden, the background being farmland, and the connecting line being water. Meanwhile, the royal garden management organization has a high level of authority. Based on the rules of the Imperial Household Department (Neiwufu) of the Qing Dynasty, the present paper extracts the royal land use and management records. This paper discusses the characteristics of royal land management from the spatial distribution and utilization of types of land. It analyzes the specific revenue, expenditure, and fund flow of land in detail and summarizes the land management model. Land management is based mainly on directional revenue and expenditure as well as quota revenue and the expenditure system. The management system is established to support the gardens’ construction.
Added Alternatives in Spoken Interaction: A Corpus Study on German Auch
Authors: Reimer Laura , Dimroth Christine
Published: October 2021

Particles such as German auch (‘also’) establish an additive relation between expressions in their scope (added constituent, AC) and context alternatives against the background of shared information (common denominator). In spoken interaction, however, explicit alternatives are not necessarily present and expressions can be construed as alternatives against different variants of a common denominator. It is the aim of the present paper to investigate to what extent the presence of alternatives influences the construction of utterances containing an additive particle. This is particularly relevant for German, where speakers can choose between an unstressed and stressed version of auch. We ask whether properties of the alternatives and their common denominators influence the choice to use stressed or unstressed auch. In a corpus study on spoken language, we classified the versions of auch, the particles AC, the alternatives in the preceding context and their common denominator. The results show that the speaker’s choice is influenced by the relation of the utterance to context alternatives. Specifically, the degree of explicitness of alternatives, the number of alternatives, and the degree of abstractness of the common denominator influence the continuation of the discourse, measured by the preference for one of the two variants of the particle auch.
Cultivating a Culture of Bilingualism: Evaluating a Home Language Arts Curriculum for SIFE
Authors: Auslander Lisa , Beiting-Parrish Maggie
Published: October 2021

This study is a mixed-methods exploratory study of a Spanish Home Language Arts (HLA) pilot curriculum designed for Students with Interrupted Education (SIFE) as it was implemented across six different schools in New York State during the 2019–2020 school year before the onset of COVID-19. The focus of the study was to observe whether the HLA curriculum improved teacher practice in the increased use of the gradual release of responsibility and the curriculum-prescribed protocols. Another goal was to examine whether the use of the curriculum helped to improve student writing and bilingual literacy. A final goal of the study was to survey teachers on their perceptions of the curriculum, especially in how the lesson design fostered student engagement and collaboration with others. The results of the internal evaluation showed that the teachers improved in their practice, especially in the areas of gradual release and increased student time on task. The students were able to develop specific text analysis and writing skills using instructional protocols used in the home language and in English that were transferable across classroom contexts. In addition, the lessons encouraged students to leverage literacy skills and background knowledge in Spanish as a way to support learning new skills in both Spanish and English. Finally, the study showed that the use of the curriculum increased student engagement and collaboration in the classroom.
A Soft Voting Ensemble-Based Model for the Early Prediction of Idiopathic Pulmonary Fibrosis (IPF) Disease Severity in Lungs Disease Patients
Authors: Ali Sikandar , Hussain Ali , Aich Satyabrata , Park Moo Suk , Chung Man Pyo , Jeong Sung Hwan , Song Jin Woo , Lee Jae Ha , Kim Hee Cheol
Published: October 2021

Idiopathic pulmonary fibrosis, which is one of the lung diseases, is quite rare but fatal in nature. The disease is progressive, and detection of severity takes a long time as well as being quite tedious. With the advent of intelligent machine learning techniques, and also the effectiveness of these techniques, it was possible to detect many lung diseases. So, in this paper, we have proposed a model that could be able to detect the severity of IPF at the early stage so that fatal situations can be controlled. For the development of this model, we used the IPF dataset of the Korean interstitial lung disease cohort data. First, we preprocessed the data while applying different preprocessing techniques and selected 26 highly relevant features from a total of 502 features for 2424 subjects. Second, we split the data into 80% training and 20% testing sets and applied oversampling on the training dataset. Third, we trained three state-of-the-art machine learning models and combined the results to develop a new soft voting ensemble-based model for the prediction of severity of IPF disease in patients with this chronic lung disease. Hyperparameter tuning was also performed to get the optimal performance of the model. Fourth, the performance of the proposed model was evaluated by calculating the accuracy, AUC, confusion matrix, precision, recall, and F1-score. Lastly, our proposed soft voting ensemble-based model achieved the accuracy of 0.7100, precision 0.6400, recall 0.7100, and F1-scores 0.6600. This proposed model will help the doctors, IPF patients, and physicians to diagnose the severity of the IPF disease in its early stages and assist them to take proactive measures to overcome this disease by enabling the doctors to take necessary decisions pertaining to the treatment of IPF disease.
Hearing Loss and Cognitive Impairment: Epidemiology, Common Pathophysiological Findings, and Treatment Considerations
Authors: Bisogno Antonella , Scarpa Alfonso , Di Girolamo Stefano , De Luca Pietro , Cassandro Claudia , Viola Pasquale , Ricciardiello Filippo , Greco Antonio , De Vincentiis Marco , Ralli Massimo , Di Stadio Arianna
Published: October 2021

In recent years, there has been increasing research interest in the correlation between hearing impairment and cognitive decline, two conditions that have demonstrated a strong association. Hearing loss appears as a risk factor for cognitive impairment, especially among certain populations, notably nursing home residents. Furthermore, hearing loss has been identified as a modifiable age-related condition linked to dementia, and it has been estimated that midlife hearing loss, if eliminated, might decrease the risk of dementia in the general population. Several mechanisms have been suggested to explain the pathologic connections between hearing loss and dementia; however, clear evidence is missing, and the common pathophysiological basis is still unclear. In this review, we discussed current knowledge about the relationship between hearing loss and dementia, and future perspectives in terms of the effects of hearing rehabilitation for early prevention of cognitive decline.
Acute Inflammation in Cerebrovascular Disease: A Critical Reappraisal with Focus on Human Studies
Authors: Dias Rafael Azevedo , Dias Leonor , Azevedo Elsa , Castro Pedro
Published: October 2021

Recent attention has been focused on the field of inflammatory biomarkers associated with vascular disorders, regarding diagnosis, prognosis, and possible therapeutical targets. In this study, we aimed to perform a comprehensive review of the literature regarding the use of inflammatory biomarkers in stroke patients. We searched studies that evaluated inflammation biomarkers associated with Cerebrovascular Disease (CVD), namely, ischemic Stroke (IS), Intracerebral Hemorrhage (ICH) and Cerebral Venous Thrombosis (CVT). As of today, neutrophil–lymphocyte ratio (NLR) seems the be the most widely studied and accepted biomarker for cerebrovascular disease due to its easy access and availability. Although demonstrated as a prognostic risk factor, in IS, ICH and CVT, its diagnostic role is still under investigation. Several other prognostic factors could be used or even combined together into a diagnostic or prognostic index. Multiple inflammatory biomarkers appear to be involved in IS, ICH, and CVT. Blood inflammatory cells, easily measured and accessible at admission may provide information regarding accurate diagnosis and prognosis. Although not yet a reality, increasing evidence exists to suggest that these may become potential therapeutic targets, likely influencing or mitigating complications of CVD and improving prognosis. Nevertheless, further larger, well-designed randomized clinical trials are still needed to follow up this hypothesis.
Properties of Biocomposites Produced with Thermoplastic Starch and Digestate: Physicochemical and Mechanical Characteristics
Authors: Ekielski Adam , Żelaziński Tomasz , Mishra Pawan Kumar , Skudlarski Jacek
Published: October 2021

This paper presents the results of a study on the influence of the addition of digestate (DG) sludge from an agricultural biogas plant on the mechanical properties of the coating obtained from thermoplastic starch (TPS). The dried, fragmented digestate, some of which had previously undergone ultrasound treatment, is used in the study. Biocomposites are produced by the pouring method using Teflon moulds as matrices. The physicomechanical study included the determination of the basic parameters of the materials obtained. Strength parameters, the contact angle, thermogravimetric properties (TGA), colour and colour difference and moisture absorption are determined. Photographs of the surface of the samples are taken with a scanning electron microscope (SEM) as well. It is found that the addition of the digestate has an advantageous effect on improving the physical and mechanical parameters. In general, samples with digestate also have a higher strength compared to the pure TPS material. The highest tensile strength and Young’s modulus are found in samples with the 14 wt% addition of ultrasound-treated digestate. On the basis of this study, it can be concluded that the addition of digestate is a promising approach for the production of TPS biocomposites with superior mechanical properties.
Residual Strength and Drying Behavior of Concrete Reinforced with Recycled Steel Fiber from Tires
Authors: Revuelta David , Carballosa Pedro , García Calvo José Luis , Pedrosa Filipe
Published: October 2021

Fiber reinforcement of concrete is an effective technique of providing ductility to concrete, increasing its flexural residual strength while reducing its potential for cracking due to drying shrinkage. There are currently a wide variety of industrial fibers on the market. Recycled steel fibers (RSF) from tires could offer a viable substitute of industrialized fibers in a more sustainable and eco-friendly way. However, mistrust exists among users, based on fear that the recycling process will reduce the performance, coupled with the difficulty of characterization of the geometry of the RSF, as a consequence of the size variability introduced by the recycling process. This work compares the behavior of RSF from tires compared with industrialized steel or polypropylene fibers, evaluating the fresh state, compressive strength, flexural residual strength, and drying behavior. The concept of Equivalent Fiber Length (EFL) is also defined to help the statistical geometrical characterization of the RSF. A microstructural analysis was carried out to evaluate the integration of the fiber in the matrix, as well as the possible presence of contaminants. The conclusion is reached that the addition of RSF has a similar effect to that of industrialized fibers on concrete’s properties when added at the same percentage.