bluuebunny/crossref_metadata_2025 huggingface dataset:
Plant diversity influenced gross nitrogen mineralization, microbial ammonium consumption and gross inorganic N immobilization in a grassland experiment
Authors: Lama Soni , Velescu Andre , Leimer Sophia , Weigelt Alexandra , Chen Hongmei , Eisenhauer Nico , Scheu Stefan , Oelmann Yvonne , Wilcke Wolfgang
Published: July 2020

Gross rates of nitrogen (N) turnover inform about the total N release and consumption. We investigated how plant diversity affects gross N mineralization, microbial ammonium (NH4+) consumption and gross inorganic N immobilization in grasslands via isotopic pool dilution. The field experiment included 74 plots with 1–16 plant species and 1–4 plant functional groups (legumes, grasses, tall herbs, small herbs). We determined soil pH, shoot height, root, shoot and microbial biomass, and C and N concentrations in soil, microbial biomass, roots and shoots. Structural equation modeling (SEM) showed that increasing plant species richness significantly decreased gross N mineralization and microbial NH4+ consumption rates via increased root C:N ratios. Root C:N ratios increased because of the replacement of legumes (low C:N ratios) by small herbs (high C:N ratios) and an increasing shoot height, which was positively related with root C:N ratios, with increasing species richness. However, in our SEM remained an unexplained direct negative path from species richness to both N turnover rates. The presence of legumes increased gross N mineralization, microbial NH4+ consumption and gross inorganic N immobilization rates likely because of improved N supply by N2 fixation. The positive effect of small herbs on microbial NH4+ consumption and gross inorganic N immobilization could be attributed to their increased rhizodeposition, stimulating microbial growth. Our results demonstrate that increasing root C:N ratios with increasing species richness slow down the N cycle but also that there must be additional, still unidentified processes behind the species richness effect potentially including changed microbial community composition.
Everyday geographies in the changing city: subjective photo-routes in Mandalay, Myanmar
Authors: Puttilli Matteo
Published: July 2020

This work upholds the importance of questioning young people to find out their point of view on urban transformation and grasp their perception and representation of the changes. Hence, the paper presents the methodology and results of an autophotography activity, and more specifically photo-routing organized in March 2018 with a group of young undergraduates at Mandalay University, Myanmar. In the sphere of this activity, the participants were asked to reflect on their relationship with the urban space and to use a camera to capture significant places and situations in their everyday experience of the city, with the goal of exploring their personal point of view on the changes occurring in it, both from a tangible and intangible perspective, in addition to how they are reflected in the everyday practice of the city. The idea at the basis of the research-intervention is that the focus on everyday life through qualitative and visual investigation techniques enables the emergence of some of the—both conscious and not—more-than-representational ways in which people—a group of young adults in this specific case—perceive and live the processual nature of the city. Results show that the photo-route tool proved to be particularly effective in stimulating a critical gaze on the city and the changes underway, to acquire awareness of the constantly in-becoming nature of the places and reflect in an introspective manner on their own life course in relation to the city. Thus, the paper provides a contribution “from below” to the reflections on urban transformations going on in Southeast Asian cities and, more precisely, in Myanmar.
The photopic negative response (PhNR): measurement approaches and utility in glaucoma
Authors: Prencipe Matteo , Perossini Tommaso , Brancoli Giampaolo , Perossini Mario
Published: July 2020

Purpose

Visual electrophysiological testing continues to generate interest among glaucoma experts because of its potential help in clarifying disease pathophysiology and promoting early detection of glaucomatous damage. The photopic negative response (PhNR) is a slow negative component of the full-field electroretinogram that has been shown to provide specific information about retinal ganglion cells (RGCs) activity. The purpose of this article is to review the literature to explore the currently available measurement methods and the utility of PhNR in glaucoma diagnostic process.

Methods

We gathered publications related to the origins, types of stimuli used, measurements methods and applications of the PhNR of ERG in animal models and humans through a search of the literature cited in PubMed. Search terms were: “PhNR”, “photopic negative response”, “glaucoma”, “glaucomatous optic neuropathy”, “ERG”, “electroretinogram”.

Results

The most reliable PhNR measurements are obtained using a red stimulus on a blue background, without requiring refractive correction, fixation monitoring, or ocular media transparency. Given its direct correlation with RGCs response, the PhNR measured as baseline-to-trough (BT) represents the most reliable parameter of evaluation. Glaucoma patients with evident perimetric defects show pathologic PhNR values. Even though the PhNR is promising in detecting early RGCs impairment, distinguishing between healthy subjects and suspect patients at risk of developing glaucomatous damage still remains challenging.

Conclusion

The PhNR is a useful additional tool to explore disorders that affect the innermost retina, including glaucoma and other forms of optic neuropathy. In particular, comparing reports of the standard examinations (optic disc assessment, OCT RNFL measurement, standard automated perimetry) with the results of electrophysiological tests may be helpful in solving clinical diagnostic and management dilemmas. On the one hand, the PhNR of the ERG can examine the parvocellular pathways; on the other hand, the steady-state pattern ERG optimized for glaucoma screening (PERGLA) can explore the magnocellular pathways. This could give ophthalmologists a useful feedback to identify early RGCs alterations suggestive of glaucoma, stratify the risk and potentially monitor disease progression.
An exploratory study of technological knowledge when pupils are designing a programmed technological solution using BBC Micro:bit
Authors: Cederqvist Anne-Marie
Published: July 2020

Technology educators often provide activities where pupils design programmed technological solutions (PTS) with various programming materials for developing pupils’ technological knowledge related to PTS and digital technology. However, few studies have investigated how pupils experience these activities. To fill this gap in knowledge, this phenomenographic study explores how pupils experience designing a PTS using BBC Micro:bit and identifies the technological knowledge these pupils need, in terms of critical aspects (i.e., aspects necessary to discern), to successfully solve a real world task—the design and coding of a burglar alarm. The data were gathered from sketches, interviews, and video-recordings of pupils aged 10 and 14. This study shows that the pupils struggled with two intertwined phenomena during the activity: the dual nature of the PTS (i.e., the structure and function) and the BBC Micro:bit material. The findings indicated that the pupils needed to understand what components to use based on their function and how to organise these components so they interacted with a code that used feedback control. That is, the pupils needed to code a conditional statement by combining blocks in the BBC Micro:bit editor. To produce working code, the pupils needed to know what the blocks represent, where to find the blocks in the editor, and how to interpret the shapes of the blocks. The results have implications for teaching technological knowledge, suggesting an importance of addressing these phenomena and critical aspects with respect to developing pupils’ conceptual and procedural knowledge related to designing PTS with BBC Micro:bit.
Simulating Strong Practical Proof Systems with Extended Resolution
Authors: Kiesl Benjamin , Rebola-Pardo Adrián , Heule Marijn J. H. , Biere Armin
Published: July 2020

Proof systems for propositional logic provide the basis for decision procedures that determine the satisfiability status of logical formulas. While the well-known proof system of extended resolution—introduced by Tseitin in the sixties—allows for the compact representation of proofs, modern SAT solvers (i.e., tools for deciding propositional logic) are based on different proof systems that capture practical solving techniques in an elegant way. The most popular of these proof systems is likely DRAT, which is considered the de-facto standard in SAT solving. Moreover, just recently, the proof system DPR has been proposed as a generalization of DRAT that allows for short proofs without the need of new variables. Since every extended-resolution proof can be regarded as a DRAT proof and since every DRAT proof is also a DPR proof, it was clear that both DRAT and DPR generalize extended resolution. In this paper, we show that—from the viewpoint of proof complexity—these two systems are no stronger than extended resolution. We do so by showing that (1) extended resolution polynomially simulates DRAT and (2) DRAT polynomially simulates DPR. We implemented our simulations as proof-transformation tools and evaluated them to observe their behavior in practice. Finally, as a side note, we show how Kullmann’s proof system based on blocked clauses (another generalization of extended resolution) is related to the other systems.
Iterative Grossone-Based Computation of Negative Curvature Directions in Large-Scale Optimization
Authors: De Leone Renato , Fasano Giovanni , Roma Massimo , Sergeyev Yaroslav D.
Published: July 2020

We consider an iterative computation of negative curvature directions, in large-scale unconstrained optimization frameworks, needed for ensuring the convergence toward stationary points which satisfy second-order necessary optimality conditions. We show that to the latter purpose, we can fruitfully couple the conjugate gradient (CG) method with a recently introduced approach involving the use of the numeral calledGrossone. In particular, recalling that in principle the CG method is well posed only when solving positive definite linear systems, our proposal exploits the use of grossone to enhance the performance of the CG, allowing the computation of negative curvature directions in the indefinite case, too. Our overall method could be used to significantly generalize the theory in state-of-the-art literature. Moreover, it straightforwardly allows the solution of Newton’s equation in optimization frameworks, even in nonconvex problems. We remark that our iterative procedure to compute a negative curvature direction does not require the storage of any matrix, simply needing to store a couple of vectors. This definitely represents an advance with respect to current results in the literature.
Roadmap for Effective School-Based Practices to Support Expectant and Parenting Youth: Lessons from the New Heights Program in Washington, DC
Authors: Asheer Subuhi , Zief Susan , Neild Ruth
Published: July 2020

Introduction

New Heights is a voluntary school-based program that provides a well-defined system of supports for expectant and parenting students in Washington, DC, and was found to be effective at improving educational outcomes. This study explores the program elements and practices that, when used together, improved academic outcomes for New Heights participants and define a possible roadmap for service providers interested in replicating the program’s success.

Methods

The study team collected data through site visits, key informant interviews, staff surveys, program observations, case files, and program materials.

Results

The core design and implementation elements of the New Heights program are (1) placing a trained staff member in the school to provide advocacy, case management, education, and in-kind incentives; (2) bringing community-based service providers into the school; (3) giving trained staff autonomy and a strong grounding in local context; and (4) using a highly collaborative process to hire and support school-based coordinators.

Discussion

Staff and funders interested in improving outcomes for young parents in school could use the experience of New Heights and the key practices that were critical to its success as a guide: (1) ensure that the program is well defined but can be tailored to the needs of schools and students, (2) engage community partners to bring services to participants, (3) hire and train the right staff who are committed to “do whatever it takes,” (4) actively cultivate a culture of collaboration among program staff, and (5) develop buy-in with school staff and illustrate program value.
Adjustability of exposed elements by updating their capacity for resistance after a damaging event: application to an earthquake–tsunami cascade scenario
Authors: Negulescu Caterina , Benaïchouche Abed , Lemoine Anne , Le Roy Sylvestre , Pedreros Rodrigo
Published: July 2020

The 2011 Great East Japan earthquake and tsunami remind us once again that these types of cascade event can occur and cause considerable damage. The scientific community realizes the need for rapid theoretical and practical progress on cascade events to provide field teams with the necessary tools and information for action during these types of events. The earthquake damage scenario for Martinique and Guadeloupe islands (French West Indies) has already been performed within the framework of French governmental projects, but these areas, in the vicinity of the French West Indies subduction zone, are also subject to tsunami events. In this study, we propose to perform a combined scenario in which an earthquake is followed by a tsunami, as it could arrive one day, considering the seismic characteristics and potential of such a subduction zone. The vulnerability of the buildings is defined considering local specific information based on several years of field inventories and inspections and is later classified into one of the 36 model building types of HAZUS. The calculation of the damages due to tsunamis follows the HAZUS methodologies. The main novelty of our study is the calculation of damage due to the two phenomena occurring one after the other, not in parallel, as is calculated in the existing literature. Therefore, for the calculation of the damages due to the second event (i.e. the tsunami), the vulnerability characteristics of the initial structure are reduced, considering the damage state of the construction after the first event (i.e. the earthquake). Hence, in our case, this calculation approach allows us to update the number of exposed elements and their changed vulnerabilities considering the damages due to the earthquake, since certain structures are already damaged by the earthquake before the arrival of the tsunami wave. The results coming from our study and our manner of treating the cascading hazards are putting into perspective with the Hazus method for combining damages coming from earthquake and the damages coming from consequently tsunami. The results expressed as the sum of the damages in both most damaged states, Extensive and Complete, are more or less in the same range of values for both studies (our study and HAZUS 2017). However, a trend of having more percentage of complete damages (and hence, less the Extensive damages) with our method than the ones obtained with the Hazus combination can be important information for crisis managing. This is a first result for the French West Indies territory, but anyway, more studies should be carried out in order to check this trend and eventually to confirm and validate this issue for others territories with others bathymetries, vulnerabilities and seismological features.
Solvent-Assisted Secondary Drying of Spray-Dried Polymers
Authors: Shepard Kimberly B. , Dower April M. , Ekdahl Alyssa M. , Morgen Michael M. , Baumann John M. , Vodak David T.
Published: July 2020

Purpose

The purpose of this work is to introduce solvent-assisted secondary drying, a method used to accelerate the residual solvent removal from spray dried materials. Spray-drying is used to manufacture amorphous solid dispersions, which enhance the bioavailability of active pharmaceutical ingredients (APIs) with low aqueous solubility. In the spray-drying process, API and excipients are co-dissolved in a volatile organic solvent, atomized into droplets through a nozzle, and introduced to a drying chamber containing heated nitrogen gas. The product dries rapidly to form a powder, but small amounts of residual solvent (typically, 1 to 10 wt%) remain in the product and must be removed in a secondary-drying process. For some spray-dried materials, secondary drying by traditional techniques can take days and requires balancing stability risks with process time.

Methods

Spray-dried polymers were secondary dried, comparing the results for three state-of-the-art methods that employed a jacketed, agitated-vessel dryer: (1) vacuum-only drying, (2) water-assisted drying, or (3) methanol-assisted drying. Samples of material were pulled at various time points and analyzed by gas chromatography (GC) and Karl Fischer (KF) titration to track the drying process.

Results

Model systems were chosen for which secondary drying is slow. For all cases studied, methanol-assisted drying outperformed the vacuum-only and water-assisted drying methods.

Conclusions

The observation that methanol-assisted drying is more effective than the other drying techniques is consistent with the free-volume theory of solvent diffusion in polymers.
Sample size in bibliometric analysis
Authors: Rogers Gordon , Szomszor Martin , Adams Jonathan
Published: July 2020

While bibliometric analysis is normally able to rely on complete publication sets this is not universally the case. For example, Australia (in ERA) and the UK (in the RAE/REF) use institutional research assessment that may rely on small or fractional parts of researcher output. Using the Category Normalised Citation Impact (CNCI) for the publications of ten universities with similar output (21,000–28,000 articles and reviews) indexed in the Web of Science for 2014–2018, we explore the extent to which a ‘sample’ of institutional data can accurately represent the averages and/or the correct relative status of the population CNCIs. Starting with full institutional data, we find a high variance in average CNCI across 10,000 institutional samples of fewer than 200 papers, which we suggest may be an analytical minimum although smaller samples may be acceptable for qualitative review. When considering the ‘top’ CNCI paper in researcher sets represented by DAIS-ID clusters, we find that samples of 1000 papers provide a good guide to relative (but not absolute) institutional citation performance, which is driven by the abundance of high performing individuals. However, such samples may be perturbed by scarce ‘highly cited’ papers in smaller or less research-intensive units. We draw attention to the significance of this for assessment processes and the further evidence that university rankings are innately unstable and generally unreliable.
Reactivity and Stability of Ultrathin VOx Films on Pt(111) in Catalytic Methanol Oxidation
Authors: von Boehn Bernhard , Scholtz Lena , Imbihl Ronald
Published: July 2020

The growth of ultrathin layers of VOx (< 12 monolayers) on Pt(111) and the activity of these layers in catalytic methanol oxidation at 10−4 mbar have been studied with low-energy electron diffraction, Auger electron spectroscopy, rate measurements, and with photoemission electron microscopy. Reactive deposition of V in O2 at 670 K obeys a Stranski–Krastanov growth mode with a (√3 × √3)R30° structure representing the limiting case for epitaxial growth of 3D-VOx. The activity of VOx/Pt(111) in catalytic methanol oxidation is very low and no redistribution dynamics is observed lifting the initial spatial homogeneity of the VOx layer. Under reaction conditions, part of the surface vanadium diffuses into the Pt subsurface region. Exposure to O2 causes part of the V to diffuse back to the surface, but only up to one monolayer of VOx can be stabilized in this way at 10−4 mbar.
Tracking of Tumor Cell–Derived Extracellular Vesicles In Vivo Reveals a Specific Distribution Pattern with Consecutive Biological Effects on Target Sites of Metastasis
Authors: Gerwing Mirjam , Kocman Vanessa , Stölting Miriam , Helfen Anne , Masthoff Max , Roth Johannes , Barczyk-Kahlert Katarzyna , Greune Lilo , Schmidt M. Alexander , Heindel Walter , Faber Cornelius , König Simone , Wildgruber Moritz , Eisenblätter Michel
Published: July 2020

Purpose

Extracellular vesicles, small vesicles carrying inter alia proteins, miRNA and RNA, are important mediators of intercellular communication. The purpose of this study was to assess the distribution of extracellular vesicles from highly malignant breast cancer and their subsequent effect on the immune cell infiltrate in target organs of metastasis.

Procedures

Extracellular vesicles were isolated from the tissue culture supernatant of highly malignant 4T1 breast cancer cells or the serum of healthy BALB/c mice. The purity of the isolate was verified by electron microscopy and western blotting. Extracellular vesicles were additionally subjected to proteome analysis. After labeling with the fluorescent dye DiR, extracellular vesicles were injected into healthy BALB/c mice and their in vivo distribution was assessed using fluorescence reflectance imaging (FRI). Following ex vivo imaging of the organs, lung tissue samples were analyzed for extracellular vesicle-mediated changes of myeloid cells and T cell numbers, using flow cytometry. Proteome analysis revealed major differences in the cargo of tumor cell–derived versus extracellular vesicles from healthy serum.

Results

In contrast to control extracellular vesicles, DiR-labeled extracellular vesicles from tumor cells preferentially accumulated in lung, liver, and spine. Subsequent flow cytometry of the immune cell composition of lung tissue samples revealed an increase of cytotoxic CD8+ T cells and a decrease of CD4+ T-helper cells as well as an increase in mature macrophages in response to tumor cell EV.

Conclusions

In conclusion, distribution of tumor cell–derived extracellular vesicles follows a specific pattern and can be monitored, using dedicated imaging. Extracellular vesicles alter the immune cell composition in target organs of metastasis, using a specific proteome cargo.
Automatic quality measurement of aortic contrast-enhanced CT angiographies for patient-specific dose optimization
Authors: Pallenberg René , Fleitmann Marja , Soika Kira , Stroth Andreas Martin , Gerlach Jan , Fürschke Alexander , Barkhausen Jörg , Bischof Arpad , Handels Heinz
Published: July 2020

Purpose

Iodine-containing contrast agent (CA) used in contrast-enhanced CT angiography (CTA) can pose a health risk for patients. A system that adjusts the frequently used standard CA dose for individual patients based on their clinical parameters can be useful. As basis the quality of the image contrast in CTA volumes has to be determined, especially to recognize excessive contrast induced by CA overdosing. However, a manual assessment with a ROI-based image contrast classification is a time-consuming step in everyday clinical practice.

Methods

We propose a method to automate the contrast measurement of aortic CTA volumes. The proposed algorithm is based on the mean HU values in selected ROIs that were automatically positioned in the CTA volume. First, an automatic localization algorithm determines the CTA image slices for certain ROIs followed by the localization of these ROIs. A rule-based classification using the mean HU values in the ROIs categorizes images with insufficient, optimal and excessive contrast.

Results

In 95.89% (70 out of 73 CTAs obtained with the ulrich medical CT motion contrast media injector) the algorithm chose the same image contrast class as the radiological expert. The critical case of missing an overdose did not occur with a positive predicative value of 100%.

Conclusion

The resulting system works well within our range of considered scan protocols detecting enhanced areas in CTA volumes. Our work automized an assessment for classifying CA-induced image contrast which reduces the time needed for medical practitioners to perform such an assessment manually.
MicroRNA Expression Signature in Mild Cognitive Impairment Due to Alzheimer’s Disease
Authors: De Felice Bruna , Montanino Concetta , Oliva Mariano , Bonavita Simona , Di Onofrio Valeria , Coppola Cinzia
Published: July 2020

Mild cognitive impairment (MCI) defines an intermediate state between normal ageing and dementia, including Alzheimer’s disease (AD). Identification of MCI subjects who will progress to AD (MCI-AD) is today of crucial importance, especially in light of the possible development of new pathogenic therapies. Several evidences suggest that miRNAs could play relevant roles in the biogenesis of AD, and the links between selected miRNAs and specific pathogenic aspects have been partly explored. In this study, we analysed the composition of microRNA transcriptome in blood, serum and cerebrospinal fluid samples from MCI-AD subjects, from an enriched small RNA library. Real-time qPCR from MCI-AD and AD patients and normal controls was performed to profile miRNA expression. In particular, four microRNAs, hsa-mir-5588-5p, hsa-mir-3658, hsa-mir-567 and hsa-mir-3908, among all selected microRNAs, are dysregulated. Hsa-mir-567 was found to be differentially expressed in cerebrospinal fluid samples, blood and serum from MCI-AD patients, showing the highest fold change and statistical significance. Target prediction analysis have been performed to evaluate mRNAs whose expression was controlled by miRNAs found to be dysregulated here, showing that hsa-mir-567 target genes are functionally active in neuronal cells. We propose that miRNA profiles found in samples from MCI-AD patients might be relevant for a better understanding of AD-related cognitive decline and could lead to set up suitable and potential biomarkers for MCI-AD progression to AD.
Quantifying the Benefits of Estuarine Habitat Restoration in the Gulf of Mexico: an Introduction to the Theme Section
Authors: Carle Melissa Vernon , Benson Kristopher G. , Reinhardt James F.
Published: July 2020

This collection of papers provides insights into methods and data currently available to quantify the benefits associated with estuarine habitat restoration projects in the northern Gulf of Mexico, USA, with potential applicability to other coastal systems. Extensive habitat restoration is expected to occur in the northern Gulf of Mexico region over the next several decades through funding associated with the 2010 Deepwater Horizon oil spill. Papers in this section examine the development of vegetation, soil properties, invertebrate fauna, and nekton communities in restored coastal marshes and provide a conceptual framework for applying these findings to quantify the benefits associated with compensatory marsh restoration. Extensive meta-analysis of existing data for Gulf of Mexico coastal habitats further confirms that structured habitats such as marsh, submerged aquatic vegetation, and oyster reefs support greater nekton densities than nonvegetated bottom habitat, with oyster reefs supporting different species assemblages than marsh and submerged aquatic vegetation. Other papers demonstrate that while vegetation cover can establish rapidly within the first 5 years of restoration, belowground parameters such as root biomass and soil organic matter remain 44% to 92% lower at restored marshes than reference marshes 15 years after restoration. On average, amphipod and nekton densities are also not fully restored until at least 20 and 13 years following restoration, respectively. Additional papers present methods to estimate the benefits associated with marsh restoration projects, nekton productivity associated with coastal and estuarine habitats, and the benefits associated with the removal of derelict crab traps in Gulf of Mexico estuaries.
The edge of the Empire: diet characterization of medieval Rome through stable isotope analysis
Authors: Varano Sara , De Angelis Flavio , Battistini Andrea , Brancazi Luca , Pantano Walter , Ricci Paola , Romboni Marco , Catalano Paola , Gazzaniga Valentina , Lubritto Carmine , Santangeli Valenzani Riccardo , Martínez-Labarga Cristina , Rickards Olga
Published: July 2020

This paper aims to define the dietary profile of the population of early medieval Rome (fifth–eleventh centuries CE) by carbon and nitrogen stable isotope analysis. This period was characterized by deep changes in the city’s economic, demographic, and social patterns, probably affecting its inhabitants’ nutritional habits. Carbon and nitrogen stable isotope analysis of bone collagen was used to detect the nutritional profile of 110 humans from six communities inhabiting the city center of Rome and one from the ancient city of Gabii. Thirteen faunal remains were also analyzed to define the ecological baseline of the medieval communities. The isotopic results are consistent with a diet mainly based on the exploitation of C3plant resources and terrestrial fauna, while the consumption of aquatic resources was detected only among the San Pancrazio population. Animal protein intake proved to be similar both among and within the communities, supporting a qualitatively homogenous dietary landscape in medieval Rome. The comparison with isotopic data from the Imperial Age allowed us to detect a diachronic nutritional transition in ancient Rome, in which the collapse of the Empire, and in particular the crisis of economic power and the trade system, represented a tipping point for its population’s nutritional habits.
Quantitative evaluation of watershed attributes for water resources management in the Rift Valley Lakes Basin, Ethiopia: a case from Tikur Wuha river watershed
Authors: Girma Rediet , Abraham Tesfalem , Muluneh Alemayehu
Published: July 2020

Characterization of watershed hydrological process is vital for sustainable water resource management. The principal goal of this study was to investigate the inference of drainage attributes on basic hydrological processes using spatial-based morphometric analysis on Tikur Wuha river watershed. The result obtained indicated that the area was characterized with fifth-order stream. Drainage area with higher stream order has lower infiltration capacity, and the shorter stream lengths were associated with the steepness of the area which affects water flow. Based on Nu value, sub-watersheds were categorized in the active erosion stage (SW7) and matured topography development (SW6). The interpretation from watershed geometry identified circular areas most susceptible to rapid hydrological response (SW11). Hydrological process and underlying materials are mainly correlated with the drainage texture parameter, and the lower the values indicated less rocky terrain and very high infiltration capacity which contributes toward less erosion (SW11). Relief parameters such as Rr value indicate the rate of stream flow and are well used in sediment yield estimation. The findings of this investigation will provide core information for water resource planning and further studies like identification of groundwater potential zones; flood risk assessment; erosion-prone area prioritization; and to select suitable sites for the construction of water harvesting structures.
Response surface modelling of the biosorption of Zn(II) and Pb(II) onto Micropogonias undulatus scales: Box–Behnken experimental approach
Authors: Ighalo Joshua O. , Eletta Omodele A. A.
Published: July 2020

In this study, the scales of Micropogonias undulatus fish were investigated as precursors for the development of low-cost biosorbent for the removal of Pb(II) and Zn(II) from aqueous media.
The biosorbent was characterised using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and X-ray diffractometry (XRD). Adsorption parameters (temperature, biosorbent dosage and contact time) were optimised using response surface methodology Box–Behnken experimental design. The optimal factors for Zn(II) removal by croaker fish scale were 145.5 min, 7.01 g/L biosorbent dosage, 30 °C and pH 5.4. The optimal factors for the removal of Pb(II) were 179.3 min, 6.61 g/L biosorbent dosage, 20 °C and pH 3.4. The numerical optimisation revealed that the optimal removal efficiency for Zn(II) and Pb(II) sorption is 96.45% and 98.76%, respectively. The biosorption of both heavy metals was best fit to Freundlich isotherm and pseudo-second-order kinetic models. Thermodynamics studies revealed that the biosorption process was exothermic and spontaneous.
Metformin inhibits pancreatic cancer metastasis caused by SMAD4 deficiency and consequent HNF4G upregulation
Authors: Wang Chengcheng , Zhang Taiping , Liao Quan , Dai Menghua , Guo Junchao , Yang Xinyu , Tan Wen , Lin Dongxin , Wu Chen , Zhao Yupei
Published: July 2020

Pancreatic ductal adenocarcinoma (PDAC) has poor prognosis due to limited therapeutic options. This study examines the roles of genome-wide association study identified PDAC-associated genes as therapeutic targets. We have identified HNF4G gene whose silencing most effectively repressed PDAC cell invasiveness. HNF4G overexpression is induced by the deficiency of transcriptional factor and tumor suppressor SMAD4. Increased HNF4G are correlated with SMAD4 deficiency in PDAC tumor samples and associated with metastasis and poor survival time in xenograft animal model and in patients with PDAC (log-rank P = 0.036; HR = 1.60, 95% CI = 1.03–2.47). We have found that Metformin suppresses HNF4G activity via AMPK-mediated phosphorylation-coupled ubiquitination degradation and inhibits in vitro invasion and in vivo metastasis of PDAC cells with SMAD4 deficiency. Furthermore, Metformin treatment significantly improve clinical outcomes and survival in patients with SMAD4-deficient PDAC (log-rank P = 0.022; HR = 0.31, 95% CI = 0.14–0.68) but not in patients with SMAD4-normal PDAC. Pathway analysis shows that HNF4G may act in PDAC through the cell-cell junction pathway. These results indicate that SMAD4 deficiency-induced overexpression of HNF4G plays a critical oncogenic role in PDAC progression and metastasis but may form a druggable target for Metformin treatment.
Update on the ESCEO recommendation for the conduct of clinical trials for drugs aiming at the treatment of sarcopenia in older adults
Authors: Reginster Jean-Yves , Beaudart Charlotte , Al-Daghri Nasser , Avouac Bernard , Bauer Jürgen , Bere Nathalie , Bruyère Olivier , Cerreta Francesca , Cesari Matteo , Rosa Mario Miguel , Cooper Cyrus , Cruz Jentoft Alfonso J. , Dennison Elaine , Geerinck Anton , Gielen Evelien , Landi Francesco , Laslop Andrea , Maggi Stefania , Prieto Yerro María Concepción , Rizzoli René , Sundseth Hildrun , Sieber Cornel , Trombetti Andrea , Vellas Bruno , Veronese Nicola , Visser Marjolein , Vlaskovska Mila , Fielding Roger A.
Published: July 2020

Background

In 2016, an expert working group was convened under the auspices of the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis (ESCEO) and formulated consensus recommendations for the conduct of clinical trials for drugs to prevent or treat sarcopenia.

Aims

The objective of the current paper is to provide a 2020 update of the previous recommendations in accordance with the evidence that has become available since our original recommendations.

Methods

This paper is based on literature reviews performed by members of the ESCEO working group and followed up with face to face meetings organized for the whole group to make amendments and discuss further recommendations.

Results

The randomized placebo-controlled double-blind parallel-arm drug clinical trials should be the design of choice for both phase II and III trials. Treatment and follow-up should run at least 6 months for phase II and 12 months for phase III trials. Overall physical activity, nutrition, co-prescriptions and comorbidity should be recorded. Participants in these trials should be at least 70-years-old and present with a combination of low muscle strength and low physical performance. Severely malnourished individuals, as well as bedridden patients, patients with extremely limited mobility or individuals with physical limitations clearly attributable to the direct effect of a specific disease, should be excluded. Multiple outcomes are proposed for phase II trials, including, as example, physical performance, muscle strength and mass, muscle metabolism and muscle-bone interaction. For phase III trials, we recommend a co-primary endpoint of a measure of functional performance and a Patient Reported Outcome Measure.

Conclusion

The working group has formulated consensus recommendations on specific aspects of trial design, and in doing so hopes to contribute to an improvement of the methodological robustness and comparability of clinical trials. Standardization of designs and outcomes would advance the field by allowing better comparison across studies, including performing individual patient-data meta-analyses, and different pro-myogenic therapies.
HOSS: an implementation of the combined finite-discrete element method
Authors: Knight Earl E. , Rougier Esteban , Lei Zhou , Euser Bryan , Chau Viet , Boyce Samuel H. , Gao Ke , Okubo Kurama , Froment Marouchka
Published: July 2020

Nearly thirty years since its inception, the combined finite-discrete element method (FDEM) has made remarkable strides in becoming a mainstream analysis tool within the field of Computational Mechanics. FDEM was developed to effectively “bridge the gap” between two disparate Computational Mechanics approaches known as the finite and discrete element methods. At Los Alamos National Laboratory (LANL) researchers developed the Hybrid Optimization Software Suite (HOSS) as a hybrid multi-physics platform, based on FDEM, for the simulation of solid material behavior complemented with the latest technological enhancements for full fluid–solid interaction. In HOSS, several newly developed FDEM algorithms have been implemented that yield more accurate material deformation formulations, inter-particle interaction solvers, and fracture and fragmentation solutions. In addition, an explicit computational fluid dynamics solver and a novel fluid–solid interaction algorithms have been fully integrated (as opposed to coupled) into the HOSS’ solid mechanical solver, allowing for the study of an even wider range of problems. Advancements such as this are leading HOSS to become a tool of choice for multi-physics problems. HOSS has been successfully applied by a myriad of researchers for analysis in rock mechanics, oil and gas industries, engineering application (structural, mechanical and biomedical engineering), mining, blast loading, high velocity impact, as well as seismic and acoustic analysis. This paper intends to summarize the latest development and application efforts for HOSS.
Lightweight in Automotive Components by Forming Technology
Authors: Rosenthal Stephan , Maaß Fabian , Kamaliev Mike , Hahn Marlon , Gies Soeren , Tekkaya A. Erman
Published: July 2020

Lightweight design is one of the current key drivers to reduce the energy consumption of vehicles. Design methodologies for lightweight components, strategies utilizing materials with favorable specific properties and hybrid materials are used to increase the performance of parts for automotive applications. In this paper, various forming processes to produce light parts are described. Material lightweight design is discussed, covering the manufacturing processes to produce hybrid components like fiber–metal, polymer–metal and metal–metal composites, which can be used in subsequent deep drawing or combined forming processes. Approaches to increasing the specific strength and stiffness with thermomechanical forming processes as well as the in situ control of the microstructure of such components are presented. Structure lightweight design discusses possibilities to plastically form high-strength or high-performance materials like magnesium or titanium in sheet, profile and tube forming operations. To join those materials and/or dissimilar materials, new joining by forming technologies are shown. To economically produce lightweight parts with gears or functional elements, incremental sheet-bulk metal forming is presented. As an important part property, the damage evolution during the forming operations will be discussed to enable even lighter parts through a more reliable design. New methods for predicting and tailoring the mechanical properties like strength and residual stresses will be shown. The possibilities of system lightweight design with forming technologies are presented. A combination of additive manufacturing and forming to produce highly complex parts with integrated functions will be shown. The integration of functions by a hot extrusion process for the manufacturing of shape memory alloys is presented. An in-depth understanding of the newly developed processes, methodologies and effects allows for a more accurate dimensioning of components. This facilitates a reduction in the total mass and an increasing performance of vehicle components.
Photoautotrophic picoplankton – a review on their occurrence, role and diversity in Lake Balaton
Authors: Somogyi Boglárka , Felföldi Tamás , Tóth László G. , Bernát Gábor , Vörös Lajos
Published: July 2020

Occurrence of the smallest phototrophic microorganisms (photoautotrophic picoplankton, APP) in Lake Balaton was discovered in the early 1980s. This triggered a series of systematic studies on APP and resulted in the setting of a unique long-term picoplankton dataset. In this review, we intend to summarize the obtained results and to give a new insight on APP ecology and diversity in Lake Balaton. According to the results, APP dynamics depends on trophic state, temperature, nutrient, and light availability, as well as grazing pressure. APP abundance in Lake Balaton decreased to a low level (1–2 × 105 cells mL−1) as a consequence of decreasing nutrient supply (oligotrophication) during the past more than two decades, and followed a characteristic seasonal dynamics with higher abundance values from spring to autumn than in winter. Concomitantly, however, the APP contribution to both phytoplankton biomass and primary production increased (up to 70% and 40–50%, respectively) during oligotrophication. Regarding annual pattern, picocyanobacteria are dominant from spring to autumn, while in winter, picoeukaryotes are the most abundant, most likely due to the different light and temperature optima of these groups. Within picocyanobacteria, single cells and microcolonies were both observed with mid-summer dominance of the latter which correlated well with the density of cladocerans. Community-level chromatic adaptation (i.e., dominance of phycoerythrin- or phycocyanin-rich forms) of planktonic picocyanobacteria was also found as a function of underwater light quality. Sequence analysis studies of APP in Lake Balaton revealed that both picocyanobacteria and picoeukaryotes represent a diverse and dynamic community consisting several freshwater genotypes (picocyanobacteria:Synechococcus, Cyanobium; picoeukaryotes:Choricystis, Stichococcus,Mychonastes, Nannochloris, andNannochloropsis).
Ten Guidelines for Better Tables
Authors: Schwabish Jonathan A.
Published: July 2020

Tables are a unique form of visualizing data because, unlike many charts, they are not usually intended to give a quick, visual representation of data. Instead, tables are useful when you want to show the exact values of your data or estimates. They are not the best solution if you want to show a lot of data or if you want to show the data in a compact space, but a well-designed table can help your reader find specific numbers and discover patterns and outliers. In this article, I present 10 guidelines for creating better, more effective tables; I then model these lessons by redesigning six tables from articles previously published in the Journal of Benefit-Cost Analysis.
Rare heterozygous GDF6 variants in patients with renal anomalies
Authors: Martens Helge , Hennies Imke , Getwan Maike , Christians Anne , Weiss Anna-Carina , Brand Frank , Gjerstad Ann Christin , Christians Arne , Gucev Zoran , Geffers Robert , Seeman Tomáš , Kispert Andreas , Tasic Velibor , Bjerre Anna , Lienkamp Soeren S. , Haffner Dieter , Weber Ruthild G.
Published: July 2020

Although over 50 genes are known to cause renal malformation if mutated, the underlying genetic basis, most easily identified in syndromic cases, remains unsolved in most patients. In search of novel causative genes, whole-exome sequencing in a patient with renal, i.e., crossed fused renal ectopia, and extrarenal, i.e., skeletal, eye, and ear, malformations yielded a rare heterozygous variant in the GDF6 gene encoding growth differentiation factor 6, a member of the BMP family of ligands. Previously, GDF6 variants were reported to cause pleiotropic defects including skeletal, e.g., vertebral, carpal, tarsal fusions, and ocular, e.g., microphthalmia and coloboma, phenotypes. To assess the role of GDF6 in the pathogenesis of renal malformation, we performed targeted sequencing in 193 further patients identifying rare GDF6 variants in two cases with kidney hypodysplasia and extrarenal manifestations. During development, gdf6 was expressed in the pronephric tubule of Xenopus laevis, and Gdf6 expression was observed in the ureteric tree of the murine kidney by RNA in situ hybridization. CRISPR/Cas9-derived knockout of Gdf6 attenuated migration of murine IMCD3 cells, an effect rescued by expression of wild-type but not mutant GDF6, indicating affected variant function regarding a fundamental developmental process. Knockdown of gdf6 in Xenopus laevis resulted in impaired pronephros development. Altogether, we identified rare heterozygous GDF6 variants in 1.6% of all renal anomaly patients and 5.4% of renal anomaly patients additionally manifesting skeletal, ocular, or auricular abnormalities, adding renal hypodysplasia and fusion to the phenotype spectrum of GDF6 variant carriers and suggesting an involvement of GDF6 in nephrogenesis.
Symmetric neural progenitor divisions require chromatin-mediated homologous recombination DNA repair by Ino80
Authors: Keil Jason M. , Doyle Daniel Z. , Qalieh Adel , Lam Mandy M. , Funk Owen H. , Qalieh Yaman , Shi Lei , Mohan Nitesh , Sorel Alice , Kwan Kenneth Y.
Published: July 2020

Chromatin regulates spatiotemporal gene expression during neurodevelopment, but it also mediates DNA damage repair essential to proliferating neural progenitor cells (NPCs). Here, we uncover molecularly dissociable roles for nucleosome remodelerIno80in chromatin-mediated transcriptional regulation and genome maintenance in corticogenesis. We find that conditionalIno80deletion from cortical NPCs impairs DNA double-strand break (DSB) repair, triggering p53-dependent apoptosis and microcephaly. Using an in vivo DSB repair pathway assay, we find thatIno80is selectively required for homologous recombination (HR) DNA repair, which is mechanistically distinct fromIno80function in YY1-associated transcription. Unexpectedly, sensitivity to loss ofIno80-mediated HR is dependent on NPC division mode:Ino80deletion leads to unrepaired DNA breaks and apoptosis in symmetric NPC-NPC divisions, but not in asymmetric neurogenic divisions. This division mode dependence is phenocopied following conditional deletion of HR geneBrca2. Thus, distinct modes of NPC division have divergent requirements forIno80-dependent HR DNA repair.