bluuebunny/crossref_metadata_2025 huggingface dataset:
Increased serum human epididymis protein 4 is associated with disease activity and systemic involvement in pediatric-onset systemic lupus erythematosus
Authors: Liu Chenxi , Yan Lingyi , Zhang Menglan , Duan Yifei , Luo Jie , Liu Zhijun , Liu Ting , Tong Jiyu , Jiang Yongmei
Published: September 2024

Objective

We aimed to investigate human epididymis protein 4 (HE4) as a potential biomarker in patients with pediatric-onset systemic lupus erythematosus (pSLE), particularly on the association of serum HE4 levels with disease activity and other laboratory tests.

Methods

We included 137 patients with pSLE and 75 age- and sex-matched healthy controls (HCs). Serum HE4 level was measured by a chemiluminescent microparticle on an Abbott ARCHITECT i2000SR Immunoassay Analyzer. Comparisons between groups were performed using the independent Student t-test, Mann–Whitney U test, Chi-square test, or Fisher’s exact test, as appropriate. We also determined the relationships between HE4 and clinical parameters and evaluated disease activity using SLE Disease Activity Index (SLEDAI) and renal SLEDAI (rSLEDAI).

Results

Serum HE4 levels in patients with pSLE (44.6 pmol/L; IQR, 32.5–73.5) were significantly higher than those in HCs (38.9 pmol/L; IQR, 34–46.1). HE4 levels were significantly higher in moderate to severe disease activities (57.4 pmol/L, IQR 37.7–164.5) than in mild disease activities (38.8 pmol/L, IQR 30.1–48.5) or HCs (38.9 pmol/L, IQR 34.0–46.1), as well as in active renal disease activities (77.2 pmol/L, IQR 47.4–224.1) than in inactive renal disease activities (36.1 pmol/L, IQR 27.8–46.7). The ROC curve analysis showed that HE4 could discriminate pSLE with renal (AUC, 0.717; 95% CI, 0.632–0.801), hematological (AUC, 0.740; 95% CI, 0.648–0.831), and cardiovascular involvement (AUC:0.775, 95% CI 0.669–0.880). Serum HE4 levels significantly correlated with several indicators related to renal morbidity, such as creatinine, blood urea nitrogen, uric acid, cystatin C, urine protein/24 h, etc.

Conclusion

Serum HE4 levels in pSLE were elevated and highly associated with disease activity and systemic involvement, indicating HE4 as a potential biomarker for pSLE.
Revisiting Thorpe scale analysis and diapycnal diffusivities in Drake Passage
Authors: Park Young-Hyang , Durand Isabelle , Lee Jae-Hak , Provost Christine
Published: September 2024

Ship heave effects in the swell-prevalent Southern Ocean induce frequent false overturns in density profiles, and conventional Thorpe scale analysis leads to unrealistically large diapycnal diffusivities. Another critical factor causing large positive biases in Thorpe scale analysis concerns the Ozmidov to Thorpe scales ratio α often considered constant and equal to 0.8. A revised Thorpe scale analysis is proposed with an innovative segment-by-segment approach to circumvent heave-induced false overturns together with best-fitting α functions to Diapycnal and Isopycnal Mixing Experiment in the Southern Ocean (DIMES) data, which yields realistic diffusivities in the Drake Passage region. The revised Thorpe scale method applied to a finely-resolved CTD section across the Drake Passage yields typical diffusivities of O (10-5-10-3 m2 s-1), with the largest values being preferentially concentrated close to the bottom at major circumpolar fronts, consistent with nearby DIMES microstructure observations. In the southern Drake Passage the method highlights a middepth (500-1500 m) mixing maximum of 10-4-10-3 m2 s-1 in the Lower Circumpolar Deep Water (LCDW) layer due to large intrusions of Warm Deep Water (a cold/fresh variety of LCDW) of Weddell Gyre origin. The inferred middepth mixing maximum in intrusive regions, which is associated with isopycnal eddy stirring rather than caused by internal waves or double diffusive mixing, supports the short-circuiting paradigm of meridional overturning circulation in the southwestern Scotia Sea region and has important climatic implications in a warming climate.
Discovering the anti-diabetic potential of pomegranate peel metabolites by examining molecular interplay with the thioredoxin-interacting protein
Authors: Aslam Tahira , Arif Amina , Arshad Shafia , Muccee Fatima , Ahmad Khalil , Iqbal Muhammad Omer , Khalil Umair , Razak Suhail , Afsar Tayyaba , Almajwal Ali , Shafique Huma , Zain Maryam
Published: September 2024

Introduction

Medicinal plants like Punica granatum (pomegranate) have traditional uses against diabetes, inflammation and other diseases. The study was initiated to get an insight into the interaction tendency of P. granatum derived compounds with diabetes associated human thioredoxin-interacting protein (TXNIP). High glucose in diabetes induces production of TXNIP resulting in β-cells apoptosis. Its inhibition might reduce the diabetes incidence.

Methods

To elucidate the therapeutic potential of P. granatum peel against diabetes through GC-MS based identification of extracted compounds followed by application of computational algorithms. P. granatum peel extracts were screened for antioxidant, anti-inflammatory, anti-diabetic, antimicrobial and wound healing properties. Phytochemical and GC-MS based analysis were performed to identify the bioactive compounds. Molecular docking analysis was performed by Auto Dock Vina to predict the binding tendency of P. granatum derived compounds with TXNIP.

Results and Discussion

The peel exhibited antioxidant, anti-inflammatory and anti-diabetic activities, which were attributed to phytochemicals like phenols, tannins and steroids. GC-MS analysis identified 3,5-octadien-2-one, 1H-pyrrole -2,5-dione, Beta-D-lyxofuranoside, 5-O-(beta-D-lyxofuranosyl)-decyl, diethyl phthalate, 9-octadecenoic acid (Z)-, methyl ester, hexadecanoic acid, methyl ester, n-hexadecanoic acid, tetradecane, 2,6,10-trimethyl, bis (2-ethylhexyl) phthalate, decane, 3,8-dimethyl, 9-octadecenoic acid (Z)-, methyl ester and bis (2-ethylhexyl) phthalate in P. granatum peel extracts. Docking analysis revealed high binding affinities of bis (2-ethylhexyl) phthalate and 9-octadecenoic acid with TXNIP i.e., –4.5 and –5.0 kcal/mol, respectively, reflecting these compounds as potent antidiabetic agents. This study validates the traditional uses of P. granatum peel and demonstrates how computational approaches can uncover pharmacologically active phytochemicals. The results suggest P. granatum peel is a promising source of novel therapeutics against diabetes, inflammation, and oxidation. Further studies on the optimization of identified ligands are warranted.
Lactiplantibacillus argentoratensis AGMB00912 protects weaning mice from ETEC infection and enhances gut health
Authors: Yoon Ki-Nam , Yang Jihye , Yeom Seo-Joon , Kim Sang-Su , Park Jong-Heum , Song Beom-Seok , Eun Jong-Bang , Park Seung-Hwan , Lee Ju Huck , Kim Hyeun Bum , Lee Ju-Hoon , Kim Jae-Kyung
Published: September 2024

Maintaining a healthy intestinal environment, optimal epithelial barrier integrity, and balanced gut microbiota composition are essential for the growth performance of weaning pigs. We identified Lactiplantibacillus argentoratensis AGMB00912 (LA) in healthy porcine feces as having antimicrobial activity against pathogens and enhanced short-chain fatty acid (SCFA) production. Herein, we assess the protective role of LA using a weaning mouse model with enterotoxigenic Escherichia coli (ETEC) infection. LA treatment improves feed intake and weight gain and alleviates colon shortening. Furthermore, LA inhibits intestinal damage, increases the small intestine villus height compared with the ETEC group, and enhances SCFA production. Using the Kyoto Encyclopedia of Genes and Genomes and other bioinformatic tools, including InterProScan and COGNIZER, we validated the presence of SCFA-producing pathways of LA and Lactiplantibacillus after whole genome sequencing. LA mitigates ETEC-induced shifts in the gut microbiota, decreasing the proportion of Escherichia and Enterococcus and increasing SCFA-producing bacteria, including Kineothrix, Lachnoclostridium, Roseuburia, Lacrimispora, Jutongia, and Blautia. Metabolic functional prediction analysis revealed enhanced functions linked to carbohydrate, amino acid, and vitamin biosynthesis, along with decreased functions associated with infectious bacterial diseases compared to the ETEC group. LA mitigates the adverse effects of ETEC infection in weaning mice, enhances growth performance and intestinal integrity, rebalances gut microbiota, and promotes beneficial metabolic functions. These findings validate the functionality of LA in a small animal model, supporting its potential application in improving the health and growth performance of weaning pigs.
Unveiling the antimicrobial and antibiofilm potential of biosurfactant produced by newly isolated Lactiplantibacillus plantarum strain 1625
Authors: Thakur Babita , Kaur Sukhminderjit , Dwibedi Vagish , Albadrani Ghadeer M. , Al-Ghadi Muath Q. , Abdel-Daim Mohamed M.
Published: September 2024

The present study aimed to characterize the biosurfactants synthesized by lactic acid bacteria (LAB) obtained from fermented foods, optimize the conditions for increasing the yield of biosurfactants and explore their antimicrobial and antibiofilm potential. Out of the 26 LAB isolates, isolate BS2 showed the highest biosurfactant production as indicated in the oil displacement test, drop collapse and emulsification activity. BS2 was identified as Lactiplantibacillus plantarum 1625 using 16S-rRNA gene sequencing and phylogenetic analysis. The biosurfactant produced by BS2 was identified as an anionic glycol-lipo-proteins by employing Fourier Transform Infrared Spectroscopy (FTIR) and Gas Chromatography–Mass Spectrometry (GC–MS) analysis. The biosurfactants produced by L. plantarum 1625 demonstrated strong antibacterial and antibiofilm characteristics against pathogenic strains such as Staphylococcus aureus MTCC 1049, Escherichia coli MTCC 1587, and Pseudomonas putida MTCC 1655. The minimal inhibition concentration value of antibacterial activity was found to be 0.1 mg/mL with the inhibition percentage ranging from 90 to 95%. Further, the effect of temperature, pH, and substrate composition on biosurfactant production was also studied to enhance it production using the Box–Behnken Design approach of Response surface methodology (RSM). Application of biosurfactant led to a considerable decrease in biofilm-forming harmful bacteria, as proven by scanning electron microscopy analysis. The results highlight the potential uses of biosurfactants in distinct industries, and biotechnological contexts, especially in the creation of new antimicrobial and antibiofilm agents.
Pathogenic role of PFKFB3 in endothelial inflammatory diseases
Authors: Zhou Ling , Li Juan , Wang Juanjuan , Niu Xuping , Li Junqin , Zhang Kaiming
Published: September 2024

The differentiation of vascular endothelial cells and the formation of new blood vessels are inseparable from the energy supply and regulation of metabolism. The budding of blood vessels is a starting point of glycolysis pathway in angiogenesis. Phosphofructokinase-2/fructose 2,6-biophosphatase 3 (PFKFB3), a key rate-limiting enzyme in glycolysis, exhibits strong kinase activity. Inhibition of PFKFB3 can reduce the rate of glycolysis, thereby inhibiting the budding of blood vessels, resulting in inhibition of pathological angiogenesis. In this review, the role of PFKFB3 in the angiogenesis of inflammatory diseases was summarized, and the endothelial inflammatory diseases associated with PFKFB3 were reviewed.
Role of stress and early-life stress in the pathogeny of inflammatory bowel disease
Authors: Bonaz Bruno , Sinniger Valérie , Pellissier Sonia
Published: September 2024

Numerous preclinical and clinical studies have shown that stress is one of the main environmental factor playing a significant role in the pathogeny and life-course of bowel diseases. However, stressful events that occur early in life, even during the fetal life, leave different traces within the central nervous system, in area involved in stress response and autonomic network but also in emotion, cognition and memory regulation. Early-life stress can disrupt the prefrontal-amygdala circuit thus favoring an imbalance of the autonomic nervous system and the hypothalamic-pituitary adrenal axis, resulting in anxiety-like behaviors. The down regulation of vagus nerve and cholinergic anti-inflammatory pathway favors pro-inflammatory conditions. Recent data suggest that emotional abuse at early life are aggravating risk factors in inflammatory bowel disease. This review aims to unravel the mechanisms that explain the consequences of early life events and stress in the pathophysiology of inflammatory bowel disease and their mental co-morbidities. A review of therapeutic potential will also be covered.
A cutting-edge new framework for the pain management in children: nanotechnology
Authors: Starcea Iuliana Magdalena , Lupu Ancuta , Nistor Ana Maria , Mocanu Maria Adriana , Bogos Roxana Alexandra , Azoicai Alice , Cira Diana , Beldie Madalina , Lupu Vasile Valeriu , Morariu Ionela Daniela , Munteanu Valentin , Tepordei Razvan Tudor , Ioniuc Ileana
Published: September 2024

Pain is a subjective concept which is ever-present in the medical field. Health professionals are confronted with a variety of pain types and sources, as well as the challenge of managing a patient with acute or chronic suffering. An even bigger challenge is presented in the pediatric population, which often cannot quantify pain in a numerical scale like adults. Infants and small children especially show their discomfort through behavioral and physiological indicators, leaving the health provider with the task of rating the pain. Depending on the pathophysiology of it, pain can be classified as neuropathic or nociceptive, with the first being defined by an irregular signal processing in the nervous system and the second appearing in cases of direct tissue damage or prolonged contact with a certain stimulant. The approach is generally either pharmacological or non-pharmacological and it can vary from using NSAIDs, local anesthetics, opiates to physical and psychological routes. Unfortunately, some pathologies involve either intense or chronic pain that cannot be managed with traditional methods. Recent studies have involved nanoparticles with special characteristics such as small dimension and large surface area that can facilitate carrying treatments to tissues and even offer intrinsic analgesic properties. Pediatrics has benefited significantly from the application of nanotechnology, which has enabled the development of novel strategies for drug delivery, disease diagnosis, and tissue engineering. This narrative review aims to evaluate the role of nanotechnology in current pain therapy, with emphasis on pain in children.
Integrative metagenomic and lipidomic analyses reveal alterations in children with obesity and after lifestyle intervention
Authors: Yin Chunyan , Liu Lujie , Xu Dong , Li Meng , Li Min , Qin Yujie , Zhang Bei , Sun Yongfa , Liu Yuesheng , Xiao Yanfeng
Published: September 2024

Background

Despite emerging evidence linking alterations in gut microbiota to childhood obesity, the metabolic mechanisms linking gut microbiota to the lipid profile during childhood obesity and weight loss remain poorly understood.

Methodology

In this study, children with obesity were treated with lifestyle weight loss therapy. Metagenomics association studies and serum untargeted lipidomics analyses were performed in children with obesity and healthy controls before and after weight loss.

Main findings

We identified alterations in gut microbiota associated with childhood obesity, as well as variations in circulating metabolite concentrations. Children with obesity showed significant decreases in the levels of s-Rothia_kristinae and s-Enterobacter_roggenkampii, alongsige elevated levels of s-Clostridiales_bacterium_Marseille-P5551. Following weight loss, the levels of s-Streptococcus_infantarius and s-Leuconostoc_citreum increased by factors of 3.354 and 1.505, respectively, in comparison to their pre-weight loss levels. Correlation analyses indicated a significant positive relationship between ChE(2:0) levels and both with s-Lachnospiraceae_bacterium_TF09-5 and fasting glucose levels. CoQ8 levels were significantly negatively correlated with s-Rothia_kristinae and HOMA-IR.

Conclusion

We linked altered gut microbiota and serum lipid levels in children with obesity to clinical indicators, indicating a potential impact on glucose metabolism via lipids. This study contributes to understanding the mechanistic relationship between altered gut microbiota and childhood obesity and weight loss, suggesting gut microbiome as a promising target for intervention.

Clinical trial registration

https://www.chictr.org.cn/showproj.html?proj=178971, ChiCTR2300072179.
Castration-resistant prostate cancer monitoring by cell-free circulating biomarkers
Authors: Chrenková Eva , Študentová Hana , Holá Kateřina , Kahounová Zuzana , Hendrychová Romana , Souček Karel , Bouchal Jan
Published: September 2024

Background

Prostate cancer is the second leading cause of male cancer-related deaths in Western countries, which is predominantly attributed to the metastatic castration-resistant stage of the disease (CRPC). There is an urgent need for better prognostic and predictive biomarkers, particularly for androgen receptor targeted agents and taxanes.

Methods

We have searched the PubMed database for original articles and meta-analyses providing information on blood-based markers for castration-resistant prostate cancer monitoring, risk group stratification and prediction of therapy response.

Results

The molecular markers are discussed along with the standard clinical parameters, such as prostate specific antigen, lactate dehydrogenase or C-reactive protein. Androgen receptor (AR) alterations are commonly associated with progression to CRPC. These include amplification of AR and its enhancer, point mutations and splice variants. Among DNA methylations, a novel 5-hydroxymethylcytosine activation marker of TOP2A and EZH2 has been identified for the aggressive disease. miR-375 is currently the most promising candidate among non-coding RNAs and sphingolipid analysis has recently emerged as a novel approach.

Conclusions

The promising biomarkers have the potential to improve the care of metastatic prostate cancer patients, however, they need further validation for routine implementation.
Bayesian optimization of proton generation in terawatt laser–CH2 cluster interactions within a plasma channel
Authors: Kim Artem , Botton Mordechai , Zigler Arie
Published: September 2024

Improving the energy efficiency in generating high-energy proton or boron ions is crucial for advancing the feasibility of neutronless laser-based proton–boron (p-B11) fusion reactions. The primary objective of this work is to optimize the fusion energy efficiency of a proposed advanced p-B11 fusion scheme. In the proposed scheme, an ultrashort laser pulse is guided by a plasma channel filled with carbon–hydrogen (CH2) clusters. The MeV protons are generated by the Coulomb explosion (CE) of the cluster, which, therefore, interact with surrounding boron to produce alpha particles. To evaluate the fusion energy efficiency under various conditions, 2D particle-in-cell (PIC) simulations are used, supplemented with analytical calculations and estimations. The Bayesian optimization (BO) algorithm is utilized to optimize the key interaction parameters. The BO approach allows us to identify optimal cluster and laser parameters that would have higher fusion energy efficiency.
The congruence between the preferred and actual places of death among terminal cancer patients in China
Authors: Lin Huijing , Ni Ping , Wu Bei , Liao Jing , Fu Jie
Published: September 2024

Background

Dying in a preferred place is important for a good death. Currently, no study has evaluated the extent to which the preferences for the place of death (PoD) are met among terminal cancer patients in China. This study examined the congruence between the preferred and actual PoD and its predictors among terminal cancer patients in China.

Methods

Between 2015 and 2023, 845 terminal cancer patients from four tertiary hospitals in Wuhan, China, were enrolled and followed till death. Face-to-face surveys at baseline and telephone-based interviews in the last month of patients’ lives were combined to learn patients’ preferred PoD. Data on patients’ actual PoD were collected from families within 1 month after patients’ death.

Results

Of the 410 patients who died, 62.7% of them died in hospitals. The agreement between patients’ preferred and actual PoD was fair (κ = 0.221). The congruence between patients’ preferred and actual PoD was 63.0, 36.6%, and 0 for hospital death, home death, and hospice facility/nursing home death separately. Patients were more likely to die in their preferred places if their preferred place and family caregivers’ belief of patients’ preferred PoD was congruent (odds ratio [OR] = 6.464, p = 0.001), or if caregivers had a medically related occupation (OR = 4.577, p = 0.003); if patients were hospitalized at least twice in the last month of life (OR = 0.422, p = 0.000), or the quality of care received by patients in the last 48 h was rated good by the families (OR = 0.373, p = 0.011), patients were less likely to die in their preferred places.

Conclusion

The congruence between patients’ preferred and actual PoD was fair. Advance care planning (ACP) needs to be popularized in China, and the quality of care in hospice facilities and nursing homes should be improved. The necessary policy support for hospice care should be made to respect cancer patients’ end-of-life (EoL) care preferences in China.
Changes in facial appearance alter one’s sensitivity not only to the self but also to the outside world
Authors: Honma Motoyasu , Yoshiba Sayaka , Miyamoto Saya , Himi Nanae , Haga Shugo , Ogura Sumire , Maki Koutaro , Masaoka Yuri , Izumizaki Masahiko , Shirota Tatsuo
Published: September 2024

Introduction

Changes in facial appearance due to orthognathic surgery are known to improve a patient’s postoperative quality of life, however, potential changes in cognitive function are unknown. This study examined the effects of changes in facial appearance due to orthognathic surgery on the sensitivity to self and to outside objects in patients with jaw deformities.

Methods

Patients with jaw deformities (n = 22) and healthy controls (n = 30) were tested at 3 months preoperatively, at 1 month preoperatively, and at 1 month postoperatively to assess their impression of objects (positive, negative, and neutral pictures) and their evaluation of their own face and body.

Results

The results showed that changes in facial appearance improved self-evaluation and increased their sensitivity to emotional objects even when the objects were identical. Furthermore, the improving rating for own face was associated with the sensitivity for objects.

Discussion

The changes in facial appearance in patients may have helped to clear the sensitivity to these emotional objects. These findings may provide a new indicator of efficacy in orthognathic surgery.
Effectiveness of school-based physical activity programs in enhancing attention, academic performance, and social relationships among children with intellectual disabilities: evidence from Pakistani schools
Authors: Sabri Saima , Zhang Mei-Yue , Guo Lu , Dang Junhua , Mao Zhi-Xiong
Published: September 2024

Physical activity (PA) offers extensive benefits for all children, including those with intellectual disabilities (ID), who face significant challenges in behavioral management and psycho-social well-being. This study investigates the effects of school-based PA on attention, academic performance, and relationships with teachers and parents in children with ID. A 12-week single-blind randomized controlled trial was conducted with 102 children with ID, aged 6 to 12 years (71 boys and 31 girls) from grades 1 to 5. Participants were divided into three groups: MVPA (moderate to vigorous PA), MPA (mild PA), and NPA (no PA). Each group engaged in PA three times a week for 45 min per session, with activities planned by a fitness trainer and supervised by the researcher. Outcome measures were assessed using SNAP-IV, STRS, CPRS, and APRS scales before and after the intervention. The results indicated that MVPA had a more significant positive impact on all outcomes compared to MPA and NPA. MPA also produced notable improvements relative to NPA. These findings underscore the importance of integrating PA into educational settings as a comprehensive strategy to enhance attention, academic performance, and social interactions for children with ID. This research highlights PA as a vital tool for addressing behavioral challenges and fostering better developmental outcomes in this population.
Experiences of individuals with serious mental disorders in regular employment through the Individual Placement and Support model
Authors: Melián Cartaya María Jesús , Arias Rodríguez Ángeles , Rodríguez Pérez Armando , Sánchez Suárez María , Rodríguez-Novo Natalia , Rodríguez-Novo Yurena , Rodríguez Pulido Francisco
Published: September 2024

Introduction

This study shows the perspective, meaning and satisfaction perceived by people with Serious Mental Disorders during their experiences in regular employment.

Methods

A mixed qualitative-quantitative methodology was used, applying semi-structured interview as qualitative information collection tool and the Indiana Job Satisfaction Scale as quantitative tool. The study period was from January 2021 to December 2022. A purposive sampling was performed with a sample of 24 people with Serious Mental Disorders who had obtained a job through an Individual Placement and Support (IPS) program during the study period. Semi-structured interview and the Indiana Job Satisfaction Scale were applied to this Serious Mental Disorder workers’ sample. The Indiana Job Satisfaction Scale was also applied to a group of 24 workers without mental disorders in the same working conditions who served as control group.

Results

The results of the analyses of the Serious Mental Disorder workers’ narratives show that perception of work experience is conditioned by individual, environmental and social predictors, as well as external factors as determining variables. Quantitative results obtained by the Indiana Job Satisfaction Scale reveal levels of job satisfaction resembling those of the rest of workers without Serious Mental Disorder.

Discussion

These findings reinforce the significance of employment in the recovery process for individuals with Serious Mental Disorders and emphasize the importance of understanding the subjective meaning individuals attribute to their work experiences.
A network approach to emotion regulation and symptom activation in depression and anxiety
Authors: Rodrigues Ana Rita , Castro Daniel , Cardoso Joana , Ferreira Filipa , Serrão Carla , Coelho Carlos M. , Meira Liliana , Ferreira Tiago B.
Published: September 2024

Background

Emotions can be regulated through several regulatory strategies that are involved in the development of psychopathological symptoms. Despite the well-established association between psychopathology and emotion dysregulation, little is known about the relationship between individual symptoms of depression and anxiety and emotion regulation strategies (ERS), as well as between ERS themselves.

Method

We conducted a cross-sectional study and examined the interactions between six ERS (reappraisal, engagement, rumination, suppression, arousal control, and distraction) and assessed their distinctive association with the activation of specific symptoms of depression and anxiety in a community sample of 376 adults (80.4% female; Mage = 32.70; SDage = 11.80). The Regulation Emotion Systems Survey (RESS) was used to measure ERS. The Patient Health Questionnaire (PHQ-9) and the Generalized Anxiety Disorder (GAD-7) were used to assess psychological symptoms. An exploratory graph analysis was performed to examine the structural properties of the network of interactions between these behaviors. Additionally, to test the association of ERS with the activation of the depression symptoms network, an expected symptoms activity (ESA) was conducted.

Results

Six communities were found that correspond to the six ERS. Rumination and suppression have a significant association with symptom activation (particularly low self-esteem), whereas reappraisal reduces symptomatic activation. The effect of arousal control, engagement, and distraction appears to depend on the remaining ERS rather than having much influence on their own.

Conclusion

This study provides insight into how ERS interact with each other and with individual symptoms of depression and anxiety. Understanding the effects of these interactions on symptom activation and comorbidity can improve our understanding of psychopathology.
Human–animal contact to inform zoonotic disease risk across gradients of agricultural land use change in the Central River Region (CRR) of The Gambia (ZooContact): a formative study
Authors: Ahmed Aliyu N. , Fornace Kimberly , Iwamura Takuya , Murray Kris A.
Published: September 2024

Introduction

Pilot studies are important initial steps in research, providing a preliminary assessment of the practicality, feasibility, and potential challenges of a proposed study. This study attempts to assess the feasibility, practicality, and acceptability of a study that integrates a human–animal contact (HAC) questionnaire, animal biodiversity survey using acoustic analysis, and zoonotic disease investigation in animals among rural households in the Central River Region (CRR) of The Gambia. The pilot study revealed granular insights that would otherwise go unnoticed, providing vital information that directly guided the design and implementation of the subsequent full-scale study on zoonotic disease risk.

Methods

A pilot study was conducted in five villages in the CRR of The Gambia. Community sensitization was carried out together with the village leadership, followed by a familiarization tour of the study setting. Questionnaire-based interview was conducted among participants (n = 50) randomly selected to assess the acceptability and reliability of the questionnaire. The feasibility and acceptability of biodiversity surveys and animal sampling were assessed using verbal inquiries from participants and community leaders.

Results

The recruitment rate was 96%, and most participants, 50 out of 52, were willing to participate without compensation for lost time during interviews. For animal sampling, 45 out of 50 participants were willing to allow the study team to sample blood and feces from their animals without any form of incentive. All five village heads agreed to the usage of sound recorders to be placed within their community for animal biodiversity assessment. For the survey effort, one field assistant interviewed 25 participants per week. It took a total of 1 h to complete an interview, including random household selection, consenting, and questionnaire interview.

Discussion

The pilot study confirmed the feasibility of the research and informed the design of the larger study. Key parameters, such as community access, acceptability, recruitment success, and logistical requirements, contributed to robust sample size calculations and realistic project cost estimates. Additionally, the study enabled the research team to familiarize themselves with the communities and refine the methods for the full study.
Personal characteristics and transmission dynamics associated with SARS-CoV-2 semi-quantitative PCR test results: an observational study from Belgium, 2021–2022
Authors: Braeye Toon , Proesmans Kristiaan , Van Cauteren Dieter , Brondeel Ruben , Hens Niel , Vermeiren Elias , Hammami Naïma , Rosas Angel , Taame Adrae , André Emmanuel , Cuypers Lize
Published: September 2024

Introduction

Following harmonization efforts by the Belgian National Reference Center for SARS-CoV-2, semi-quantitative PCR test (SQ-PCR) results, used as a proxy for viral load, were routinely collected after performing RT-qPCR tests.

Methods

We investigated both the personal characteristics associated with SQ-PCR results and the transmission dynamics involving these results. We used person-level laboratory test data and contact tracing data collected in Belgium from March 2021 to February 2022. Personal characteristics (age, sex, vaccination, and laboratory-confirmed prior infection) and disease stage by date of symptom onset were analyzed in relation to SQ-PCR results using logistic regression. Vaccine effectiveness (VE) against a high viral load (≥107 copies/mL) was estimated from the adjusted probabilities. Contact tracing involves the mandatory testing of high-risk exposure contacts (HREC) after contact with an index case. Odds ratios for test positivity and high viral load in HREC were calculated based on the SQ-PCR result of the index case using logistic regression models adjusted for age, sex, immunity status (vaccination, laboratory-confirmed prior infection), variant (Alpha, Delta, Omicron), calendar time, and contact tracing covariates.

Results

We included 909,157 SQ-PCR results of COVID-19 cases, 379,640 PCR results from index cases, and 72,052 SQ-PCR results of HREC. High viral load was observed more frequently among recent cases, symptomatic cases, cases over 25 years of age, and those not recently vaccinated (>90 days). The vaccine effectiveness (VE) of the primary schedule in the first 30 days after vaccination was estimated at 47.3% (95%CI 40.8–53.2) during the Delta variant period. A high viral load in index cases was associated with an increased test positivity in HREC (OR 2.7, 95%CI 2.62–2.79) and, among those testing positive, an increased likelihood of a high viral load (OR 2.84, 95%CI 2.53–3.19).
Parallel reaction monitoring targeted mass spectrometry as a fast and sensitive alternative to antibody-based protein detection
Authors: Bezstarosti Karel , Van der Wal Lennart , Doff Wouter A. S. , Demmers Jeroen A. A.
Published: September 2024

The reliable, accurate and quantitative targeted detection of proteins is a key technology in molecular and cell biology and molecular diagnostics. The current golden standard for targeted protein detection in complex mixtures such as complete cell lysates or body fluids is immunoblotting, a technology that was developed in the late 1970s and has not undergone major changes since. Although widespread, this methodology suffers from several disadvantages, such as the inability to detect low-abundant proteins or specific posttranslational modifications, the requirement for highly specific antibodies, the lack of quantitative power and the often-tedious practical procedures. Mass spectrometry (MS) based targeted protein detection is an alternative technology that could circumvent these caveats. Here, we compare immunoblotting with targeted protein mass spectrometry using a parallel reaction monitoring (PRM) regime on the Orbitrap mass spectrometer. We show that PRM based MS has superior sensitivity and quantitative accuracy over immunoblotting. The limit of detection for proteolytic peptides of a purified target protein was found to be in the mid- to low-attomole range and approximately one order of magnitude higher when embedded in a complex biological matrix. The incorporation of synthetic heavy isotope labeled (AQUA) peptides as internal calibrants into the PRM workflow allows for even higher accuracy for both the relative and absolute quantitation of tryptic target peptides. In conclusion, PRM is a versatile and sensitive technology, which can overcome the shortcomings of immunoblotting. We argue that PRM based MS could become the method of choice for the targeted detection of proteins in complex cellular matrices or body fluids and may eventually replace standard methods such as Western blotting and ELISA in biomedical research and in the clinic.
Digital transformation and organizational restlessness
Authors: Besio Cristina , Jöstingmeier Marco , Posner Christine
Published: September 2024

In modern society, organizations are expected to be increasingly flexible and adapt to constantly changing environments. While such flexibility is often considered a positive trait of organizations, the risks of continuous organizational change are often overlooked. Against this background, we argue that continuous, multiple and uncoordinated organizational change can lead to a state we define as “organizational restlessness” and a loss of the benefits of stable structures. Paradoxically, it is even possible that organizational restlessness reduces the capability of organizations to planfully introduce specific and highly desirable changes, such as those related to digital transformation. Using qualitative data from interviews and participant observations, we analyze a large German public administration and identify three sources of organizational restlessness: the innovation imperative of modern society, changes in political leadership as a result of democratic elections and the bureaucratic principle of personnel rotation. While barriers to digital transformation are often explained by bureaucratic rigidity, we show that also constant uncoordinated change hinders sustainable digital transformation. Our paper thus contributes to an enhanced understanding of organizational continuity and disruption, as we show that both are needed to digitalize organizations further.
Culture of care: the question of animal agency in laboratory animal science
Authors: Ameli Katharina , Krämer Stephanie
Published: September 2024

Background

A majority of the current debates in experimental animal science research focus to a large extent on the significance and implementation of the 3Rs principle according to Russell and Burch. In this context, not least due to the EU Directive 2010/63/EU, the concept of a culture of care has become more prevalent. Although animals are essential actors in the field of laboratory science, the discussion around animal agency, as well as the resulting consequences for laboratory animal science, is currently unconsidered.

Methods

The purpose of this qualitative survey was to identify the perception and understanding of professional workers in laboratory animal science regarding the culture of care in general and aspects of animal agency in particular. Using a non-standardized qualitative survey method (topic-oriented, guideline-based expert interviews), persons involved in animal experimentation in different groups (management, science, regulation, and care) were interviewed.

Results

Overall, the results of the qualitative survey showed that animal agency plays a subordinate role in the question of a culture of care in animal research. Although not all groups explicitly applied the construct of animal agency or comparable terminology for this, there were links to the theoretical construct. Overall, the interviews showed a recognized network between humans and animals and that animals can interact dialogically in research. This is justified, for example, by the transfer of emotions from carers or scientists to animals. Nevertheless, a differentiated reflection of an animal’s agency remains disregarded.

Conclusion

The present qualitative survey approached the understanding of a culture of care among experts in the field of animal research. Animal agency does occur in the theoretical reception of the culture of care model. However, it is not conclusively established in everyday practice. Rather, the results lead to the assumption that strategies are being implemented to largely fade out animal agency.
The Role of Tumor Suppressor p53 Protein in HIV–Host Cell Interactions
Authors: Bakhanashvili Mary
Published: September 2024

The virus–host relationship is indispensable for executing successful viral infection. The pathogenesis of HIV is determined by an intricate interaction between the host and the virus for the regulation of HIV infection, thereby influencing various aspects, including the regulation of signaling pathways. High mutation rates and population heterogeneity characterize HIV with consequences for viral pathogenesis and the potential to escape the immune system and anti-viral inhibitors used in therapy. The origin of the high mutation rates exhibited by HIV may be attributed to a limited template-copied fidelity that likely operates in the cytoplasm. HIV-1 infection induces upregulation and activation of tumor suppressor p53 protein in the early stages of HIV-1 infection. p53 plays a multifaceted role in the context of HIV infection, thereby affecting viral replication. p53 is involved in maintaining genetic integrity, actively participating in various DNA repair processes through its various biochemical activities and via its ability to interact with components of the repair machinery. This report focuses on the impact of the p53 protein on the HIV-1 reverse transcription process while incorporating various incorrect and non-canonical nucleotides. The presence of functional host-coded p53 protein with proofreading–repair activities in the cytoplasm may lead to various biological outcomes.
COVID-19 and Cancer Care: A Review and Practical Guide to Caring for Cancer Patients in the Era of COVID-19
Authors: Claveau Simon , Mahmood Farhan , Amir Baraa , Kwan Jennifer Jing Wah , White Cheryl , Vipond Joe , Iannattone Lisa
Published: September 2024

COVID-19, a novel infectious disease caused by the emergence of the SARS-CoV-2 virus in 2020, has had a profound impact on healthcare, both at the individual and population level. The impact at the population level was felt most acutely during the emergency phase of the pandemic, with hospital capacity issues leading to widespread disruptions and delays in the delivery of healthcare services such as screening programs and elective surgeries. While hospitals are no longer being acutely overwhelmed by COVID-19 patients, the impact of the virus on vulnerable patient populations such as cancer patients continues to be of ongoing consequence. Cancer patients remain at high risk of hospitalization, ICU admission, and death due to COVID-19, even in the era of vaccination. Infection prevention and risk mitigation strategies such air quality control, masking, testing, vaccination, and treatment should therefore be integrated into the usual care and counseling of cancer patients moving forward to avoid preventable morbidity and mortality from this infection and ensure the safety of this vulnerable cohort as they navigate their cancer diagnosis and treatment in the era of COVID-19.
Performance Analysis and Rapid Optimization of Vehicle ORC Systems Based on Numerical Simulation and Machine Learning
Authors: Wang Xin , Chen Xia , Xing Chengda , Ping Xu , Zhang Hongguang , Yang Fubin
Published: September 2024

The organic Rankine cycle (ORC) system is an important technology for recovering energy from the waste heat of internal combustion engines, which is of significant importance for the improvement of fuel utilization. This study analyses the performance of vehicle ORC systems and proposes a rapid optimization method for enhancing vehicle ORC performance. This study constructed a numerical simulation model of an internal combustion engine-ORC waste heat recovery system based on GT-Suite software v2016. The impact of key operating parameters on the performance of two organic Rankine cycles: the simple organic Rankine cycle (SORC) and the recuperative organic Rankine cycle (RORC) was investigated. In order to facilitate real-time prediction and optimization of system performance, a data-driven rapid prediction model of the performance of the waste heat recovery system was constructed based on an artificial neural network. Meanwhile, the NSGA-II multi-objective algorithm was used to investigate the competitive relationship between different performance objective functions. Furthermore, the optimal operating parameters of the system were determined by utilizing the TOPSIS method. The results demonstrate that the highest thermal efficiencies of the SORC and RORC are 6.21% and 8.61%, respectively, the highest power outputs per unit heat transfer area (POPAs) are 6.98 kW/m2 and 8.99 kW/m2, respectively, the lowest unit electricity production costs (EPC) are 7.22 × 10−2 USD/kWh and 3.15 × 10−2 USD/kWh, respectively, and the lowest CO2 emissions are 2.85 ton CO2,eq and 3.11 ton CO2,eq, respectively. The optimization results show that the RORC exhibits superior thermodynamic and economic performance in comparison to the SORC, yet inferior environmental performance.
A Review of Biodiesel Cold Flow Properties and Its Improvement Methods: Towards Sustainable Biodiesel Application
Authors: Pradana Yano Surya , Makertihartha I Gusti B. N. , Indarto Antonius , Prakoso Tirto , Soerawidjaja Tatang Hernas
Published: September 2024

Significant concerns over energy security and environmental impact reduction will drive all stakeholders to generate proper alternative energies. Biodiesel is a prospective cleaner-burning biofuel that can contribute on addressing these concerns globally. Presently, pure biodiesel (B100) application is still facing several obstacles, principally in terms of its cold flow properties. Improvement in cold flow behavior parameters is the solution to promoting biodiesel implementation at a higher percentage and wider environmental temperature range. This study provides a detailed review of several improvement methods, both physical, chemical, and biological, from various scientific sources, to elevate the cold fluidity characteristics of biodiesel. The investigated methods convincingly offer proper enhancement in the cold flow properties of biodiesel. Mostly, this improvement is accompanied by an alleviation in oxidation stability, cetane number, and/or viscosity. However, the skeletal isomerization method presents promising cold fluidity refinement with minimal reduction in other physical properties. Therefore, the continuous development of these methods promises global sustainable application of high-quality biodiesel.
Depression and Anxiety among Migrant Older Adults during the COVID-19 Pandemic in China: Network Analysis of Continuous Cross-Sectional Data
Authors: Zhang Chi , Zhao Yuefan , Wei Lei , Tang Qian , Deng Ruyue , Yan Shiyuan , Yao Jun
Published: September 2024

Many Chinese migrant older adults are more prone to mental health problems due to their “migrant” status. During the COVID-19 pandemic, restrictions on their mobility exacerbated these conditions. Mental health is a crucial dimension of healthy aging. Network analysis offers a novel method for exploring interactions between mental health problems at the symptom level. This study employs network analysis to examine the interactions between comorbid depressive and anxiety symptoms across different stages of the COVID-19 pandemic. Surveys were conducted from September 2019 to January 2020 (T1), September 2020 to January 2021 (T2), and September 2021 onwards (T3). Depression and anxiety symptoms were measured by the Patient Health Questionnaire-9 (PHQ-9) and the Hospital Anxiety and Depression Scale-Anxiety (HADS-A). Expected Influence (EI) and Bridge Expected Influence (Bridge EI) were used to identify central and bridge symptoms in the network. Network stability and accuracy tests were performed. Among the Chinese migrant older adults, the anxiety prevalence was 18.50% at T1, 21.11% at T2, and 9.38% at T3. The prevalence of depression was 26.95% at T1, 55.44% at T2, and 60.24% at T3. The primary central symptoms included ‘Afraid something will happen’ (A2), ‘Irritability’ (A6), ‘Panic’ (A7), ‘Feeling of worthlessness’ (D6), ‘Anhedonia’ (D1), and ‘Feeling of fear’ (A5). The major bridge symptoms included ‘Feeling of fear’ (A5), ‘Panic’ (A7), ‘Irritability’ (A6), ‘Fatigue’ (D4), ‘Anhedonia’ (D1), and ‘Depressed or sad mood’ (D2). Differences in network structure were observed across the periods. The network analysis further revealed the evolving relationships between central and bridge symptoms over time, highlighting the importance of targeted intervention strategies for central and bridge symptoms of comorbid depression and anxiety at different periods.