bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Stocking Density on Behavior and Pen Cleanliness of Grouped Growing Pigs
Authors: Zeng Yaqiong , Wang Hao , Ruan Rongdan , Li Yongzhen , Liu Zuohua , Wang Chaoyuan , Liu Anfang
Published: March 2022

In recent years, animal cleanliness during production has gradually attracted increasing attention. Although pigs by nature tend to excrete in dark and humid corners, in the prevalent highly intensive pig production, excessive stocking density often restricts the ability of pigs to excrete at designated points, thereby leading to pollution of the pens. To study the effect of stocking density on pen cleanliness and the relevant pig behavior, a total of 216 Landrace × Yorkshire × Duroc hybrid pigs were randomly grouped at 0.5, 0.7, or 0.9 m2 per pig at 59 ± 3 days of age. The temporal and spatial distributions of lying and excreting behavior of pigs were monitored on days 1, 7, and 35 after transfer, and the cleanliness of pig pens under three stocking densities was scored. The results showed that there were no significant differences in excreting or lying time rhythms among the three treatments. In the initial and stable periods of group transfer, the excretion rate of pigs on slatted floors was significantly higher than that on solid floors at 0.9 m2/pig (p < 0.01). During the group stabilization stage and at the end of the experiment, the lying rate of pigs on solid floors under 0.9 m2/pig was 10.81%, that is, 7.43% higher than that of 0.7 m2/pig, and the differences were significant. Judging from the pollution score of the solid floors, the three stocking densities all showed more serious pollution at the corners, and the pollution score of the pens with a density of 0.9 m2/pig was lower than that of the pens with a density of 0.5 and 0.7 m2/pig. The analysis of whether pigs had corresponding behaviors in specific functional areas showed that pigs at a density of 0.9 m2/pig had a 10.14% lower lying rate on slatted floors (the expected excretion area) than on solid floors, whereas the densities of 0.5 and 0.7 m2/pig showed the opposite pattern. Pigs at a density of 0.9 m2/pig had lower excretion rates in both corners of the solid floors (the desired lying area) than that of the 0.5 m2/pig group (p < 0.05) and 0.7 m2/pig group (p > 0.05). These results indicate that when the effective occupied space of pigs was larger, specific behaviors were more likely to occur in the set functional areas, and the cleanliness of the pen was higher. Under the conditions of this experiment, the recommended stocking density for growing pigs was 0.9 m2/pig. Of course, a larger space may be more beneficial to animal health and welfare, but the economic costs must also be considered.
Soil Dynamics and Nitrogen Absorption by a Natural Grassland under Cow Urine and Dung Patches in an Andisol in Southern Chile
Authors: Ramírez-Sandoval Magdalena , Pinochet Dante , Rivero M. Jordana
Published: March 2022

The objective of this study was to assess N dynamics in the soil, and the response in regard to dry matter yield (DM yield; kg·DM·ha−1), N yield (g·N·m−2) and N concentration (g·N·kg−1 DM) under urine patches and dung pats and in the affected zone of the grassland soil over 304 days. The amount of N under the urine and dung patches increased until day 10 and then started to decrease until the end of the experiment, reaching control treatment levels. The DM yield in the urine patch zone and the 0–10 cm zone around the dung pat was higher than in the control treatment throughout the evaluated period, while in the 0–30 cm zone around the urine patch and the 10–20 cm zone around the dung pat the DM yield was negatively affected by the application of excreta. The maximum accumulated N yield was up to two times higher than the control in the 0–10 cm zone of the dung pat, while the mean concentration of N throughout the period was 42% higher than the control for the “urine patch” zone and 47% higher in the 0–10 cm area around the dung pat. The total absorbed N was 19% and 15% for urine and dung excreta, respectively. Further research is needed to explain the variables that might affect the results obtained, and it is necessary to evaluate the botanical composition as a factor that contributes to this effect.
Intercropping Winter Oilseed Rape (Brassica napus L.) Has the Potential to Lessen the Impact of the Insect Pest Complex
Authors: Breitenmoser Stève , Steinger Thomas , Baux Alice , Hiltpold Ivan
Published: March 2022

Winter oilseed rape (Brassica napus) is a global major crop used for the production of vegetable oil. Typically sown in late summer and grown throughout winter and spring, it allows for interesting cultural practices, such as frost-sensitive intercropping with companion plants. This practice not only provides nitrogen resources much needed by the crop in the spring, but companion plants can also prevent weed growth in autumn, thereby reducing common herbicide use. Additionally, intercropping has the potential to protect the crop from insect pests. During winter 2019–2020, B. napus was grown alone (i.e., as a control) or intercropped with a mixture of faba bean (Vicia faba) and grass pea (Lathyrus sativus); because of the unusually clement weather conditions, the faba bean did not freeze, which allowed for the evaluation of the impact of these companion plants on the insect pest complex in spring. Insect damage by the beetles Psylliodes chrysocephala, Ceutorhynchus napi, and Brassicogethes aeneus was assessed in both treatments. The larval density of P. chrysocephala was significantly lower in the crop grown with service plants. Egg laying and damage by C. napi were significantly reduced when B. napus was intercropped, and the number of B. aeneus captured was significantly lower in the presence of service plants than in the control. Moreover, the yield from oilseed rape was significantly higher in the part of the field with service plants than in the pure crop control. The underlying mechanisms are only partially understood, but intercropping winter oilseed rape with frost-resistant service plants seems to be an ecologically sound practice with a very high level of potential to reduce insect pest pressure and increase crop yield. This may eventually reduce our reliance on chemical inputs in one of the most treated crops.
Rule-Enhanced Active Learning for Semi-Automated Weak Supervision
Authors: Kartchner David , Nakajima An Davi , Ren Wendi , Zhang Chao , Mitchell Cassie S.
Published: March 2022

A major bottleneck preventing the extension of deep learning systems to new domains is the prohibitive cost of acquiring sufficient training labels. Alternatives such as weak supervision, active learning, and fine-tuning of pretrained models reduce this burden but require substantial human input to select a highly informative subset of instances or to curate labeling functions. REGAL (Rule-Enhanced Generative Active Learning) is an improved framework for weakly supervised text classification that performs active learning over labeling functions rather than individual instances. REGAL interactively creates high-quality labeling patterns from raw text, enabling a single annotator to accurately label an entire dataset after initialization with three keywords for each class. Experiments demonstrate that REGAL extracts up to 3 times as many high-accuracy labeling functions from text as current state-of-the-art methods for interactive weak supervision, enabling REGAL to dramatically reduce the annotation burden of writing labeling functions for weak supervision. Statistical analysis reveals REGAL performs equal or significantly better than interactive weak supervision for five of six commonly used natural language processing (NLP) baseline datasets.
Atolla reynoldsi sp. nov. (Cnidaria, Scyphozoa, Coronatae, Atollidae): A New Species of Coronate Scyphozoan Found in the Eastern North Pacific Ocean
Authors: Matsumoto George I. , Christianson Lynne M. , Robison Bruce H. , Haddock Steven H. D. , Johnson Shannon B.
Published: March 2022

We have observed and collected unusual specimens of what we recognize as undescribed types of the genus Atolla over the past 15 years. Of these, there appear to be three potentially different types. One of these has now been genetically sequenced and compared both morphologically and molecularly with five other Atolla species that have been found in the eastern Pacific. This new variant is so morphologically distinct from other previously described Atolla species that we believe it can be described as a new species, Atolla reynoldsi sp. nov. This species along with two additional types may comprise a new genus. It is also clear that a more accurate and diagnostic morphological key for the genus Atolla needs to be developed. This paper will also provide some potential starting points for a new key to the genus.
The Effect of Lift Crates on Piglet Survival Rate and Sow Stress Level during Farrowing
Authors: Costa Annamaria , Salvagnini Cecilia , Buoio Eleonora , Palmeri Fabio , Salvagnini Andrea , Mazzola Silvia Michela
Published: March 2022

In the present study, we aimed to determine the effect of lift farrowing crates on piglet mortality by crushing and on sow welfare. Eighty-four sows were considered in the one-year experiment in three monitoring sessions. In each session, 14 sows were housed in a room with conventional crates (CC), and 14 sows were lodged in a room equipped with lift crates (LC). The sows, of the same genetics, with parity ranging from 2 to 9, were randomly distributed in CC and LC rooms. No primiparous sows were considered in the study to avoid sows unexperienced with the dynamics of lift crates. The numbers of crushed piglets, assessed by the farm’s veterinarian, within 48 h, 72 h, and at weaning (28th day), were recorded. Hair cortisol concentration (HCC) was measured upon entry and exit from farrowing to evaluate stress level variation. Feet diseases and backfat thickness were evaluated to assess sows’ potential diseases induced by lift crates and metabolic problems. The results show that the number of crushed piglets per sow was higher in the CC rooms than in the LC rooms in the first two days after delivery (0.39 vs. 0.15, p < 0.05) and up to weaning (0.50 vs. 0.37; p < 0.05). Mean values of HCC variation in sows during farrowing were significantly different in the two housing systems and higher for the LC sows (0.53 pg/mg vs. 0.22 pg/mg; p < 0.05). No significant differences were detected for backfat variation and feet disease scoring between LC and CC sows. In conclusion, LC sows evidenced an increase in hair cortisol values during farrowing, probably caused by a higher stress status induced by the lift crate, along with the benefit of the higher survival rate of piglets before weaning.
Effective Clinical Pathway Improves Interprofessional Collaboration and Reduces Antibiotics Prophylaxis Use in Orthopedic Surgery in Hospitals in Indonesia
Authors: Herawati Fauna , Irawati Adinda Dessi , Viani Ella , Sugianto Nully Andaretha , Rahmatin Nur Laili , Artika Made Prita , Sahputri Sukmawati Eka Bima , Setiasih Setiasih , Kantono Kevin , Yulia Rika , Andrajati Retnosari , Soemantri Diantha
Published: March 2022

Clinical pathways can improve the quality of health services. The effectiveness and impact of implementing clinical pathways are controversial. The preparation of clinical pathways not only enacts therapeutic guidelines but requires mutual agreement in accordance with the roles, duties, and contributions of each profession in the team. This study aimed to investigate the perception of interprofessional collaboration practices and the impact of clinical pathway implementation on collaborative and Defined Daily Dose (DDD) prophylactic antibiotics per 100 bed-days in orthopedic surgery. The Collaborative Practice Assessment Tool (CPAT) questionnaire was used as a tool to measure healthcare’ perceptions of collaborative practice. The clinical pathway (CP) in this study was adapted from existing CPs published by the Indonesian Orthopaedic Association (Perhimpunan Dokter Spesialis Orthopaedi dan Traumatologi Indonesia, PABOI) and was commended by local domestic surgeons and orthopedic bodies. We then compared post-implementation results with pre-implementation clinical pathway data using ANCOVA to explore our categorical data and its influence towards CPAT response. ANOVA was then employed for aggregated DDD per 100 bed-days to compare pre and post intervention. The results showed that the relationships among members were associated with the working length. Six to ten years of working had a significantly better relationship among members than those who have worked one to five years. Interestingly, pharmacists’ leadership score was significantly lower than other professions. The clinical pathway implementation reduced barriers in team collaboration, improved team coordination and organization, and reduced third-generation cephalosporin use for prophylaxis in surgery (pre: 59 DDD per 100 bed-days; post: 28 DDD per 100 bed-days). This shows that the clinical pathway could benefit antibiotic stewardship in improving antibiotic prescription, therefore reducing the incidence of resistant bacteria.
Thiol Reductases in Deinococcus Bacteria and Roles in Stress Tolerance
Authors: de Groot Arjan , Blanchard Laurence , Rouhier Nicolas , Rey Pascal
Published: March 2022

Deinococcus species possess remarkable tolerance to extreme environmental conditions that generate oxidative damage to macromolecules. Among enzymes fulfilling key functions in metabolism regulation and stress responses, thiol reductases (TRs) harbour catalytic cysteines modulating the redox status of Cys and Met in partner proteins. We present here a detailed description of Deinococcus TRs regarding gene occurrence, sequence features, and physiological functions that remain poorly characterised in this genus. Two NADPH-dependent thiol-based systems are present in Deinococcus. One involves thioredoxins, disulfide reductases providing electrons to protein partners involved notably in peroxide scavenging or in preserving protein redox status. The other is based on bacillithiol, a low-molecular-weight redox molecule, and bacilliredoxin, which together protect Cys residues against overoxidation. Deinococcus species possess various types of thiol peroxidases whose electron supply depends either on NADPH via thioredoxins or on NADH via lipoylated proteins. Recent data gained on deletion mutants confirmed the importance of TRs in Deinococcus tolerance to oxidative treatments, but additional investigations are needed to delineate the redox network in which they operate, and their precise physiological roles. The large palette of Deinococcus TR representatives very likely constitutes an asset for the maintenance of redox homeostasis in harsh stress conditions.
Proprotein Convertase Subtilisin Kexin Type 9 (PCSK9) Beyond Lipids: The Role in Oxidative Stress and Thrombosis
Authors: Cammisotto Vittoria , Baratta Francesco , Simeone Paola G. , Barale Cristina , Lupia Enrico , Galardo Gioacchino , Santilli Francesca , Russo Isabella , Pignatelli Pasquale
Published: March 2022

Proprotein convertase subtilisin/kexin type 9 (PCSK9), mainly secreted in the liver, is a key regulator of cholesterol homeostasis inducing LDL receptors’ degradation. Beyond lipid metabolism, PCSK9 is involved in the development of atherosclerosis, promoting plaque formation in mice and human, impairing the integrity of endothelial monolayer and promoting the events that induce atherosclerosis disease progression. In addition, the PCSK9 ancillary role in the atherothrombosis process is widely debated. Indeed, recent evidence showed a regulatory effect of PCSK9 on redox system and platelet activation. In particular, the role of PCSK9 in the activation of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (Nox2) system, of MAP-kinase cascades and of CD36 and LOX-1 downstream pathways, suggests that PCSK9 may be a significant cofactor in atherothrombosis development. This evidence suggests that the serum levels of PCSK9 could represent a new biomarker for the occurrence of cardiovascular events. Finally, other evidence showed that PCSK9 inhibitors, a novel pharmacological tool introduced in clinical practice in recent years, counteracted these phenomena. In this review, we summarize the evidence concerning the role of PCSK9 in promoting oxidative-stress-related atherothrombotic process.
Communication Transport Protocol Strategies for Rail Applications
Authors: Giuliano Romeo , Vizzarri Alessandro , Calderone Antonino , Mazzenga Franco
Published: March 2022

Current technologies for managing rail traffic such as the Global System for Mobile communications for Railway (GSM-R) will be no longer be available within the upcoming years. The European Shift2Rail Joint Undertaking (S2R-JU) proposed the Adaptable Communication System (ACS) to overcome this problem. In this work, we model the ACS by abstracting it at the Internet Protocol (IP) level, using tunnels for datagrams’ transmission as a communication bearer is available along the rail. Then, to evaluate its performance, an ACS emulator has been implemented. The core part of it is a Tunnel Manager which can establish pseudo-virtual circuits through multi-bearer tunnels, forcing datagrams on a service-basis to follow specific paths between gateways (i.e., from on-board to a train to the network-side rail control center and vice versa). The Tunnel Manager can properly select a given tunnel/bearer for sending messages (and duplicating them on redundant paths) of critical rail applications for train traffic management, relying on tunnels based on either connection-oriented protocol (i.e., the Transport Control Protocol, TCP), connectionless protocol (i.e., the User Datagram Protocol, UDP) or a mix of them. In this paper, we investigate the best solutions in terms of transport protocols for implementing tunnels through the bearers. Results are based on two main use cases: i. the position report/movement authority messages for the European Rail Traffic Management System (ERMTS) and ii. the critical file transmission, considering either TCP or UDP as tunnel transport protocol. For the first rail application, one UDP bearer can be selected only if the end-to-end channel delay is lower than 100 ms and the experienced packet loss is lower than 4% in the whole crossed network. Two UDP bearers, one TCP bearer or two mixed UDP/TCP bearers should be selected in case the channel delay is greater than 300 ms and the experienced packet loss is greater than 15%. Considering the critical file transfer in the rail scenario, TCP should be selected with two bearers to have a throughput greater than 50 Mbit/s even for a packet loss of 1%.
Real-Time Measurement of Photodissociation with a Static Modulated Fourier Transform Spectrometer
Authors: Cho Ju Yong , Kim Hyoungjin , Lee Seunghoon , Jang Won Kweon
Published: March 2022

A static modulated Fourier transform spectrometer composed of a modified Sagnac interferometer was implemented for real-time remote sensing of the spectral property changes in a solid dye. In the spectrum obtained from the implemented spectrometer, the relationship between spectral resolution and dependent factors was discussed to prevent aliasing. As a target material, a solid-state dye of rhodamine-6G was fabricated in the laboratory. When an intense pumping laser light was irradiated to a solid dye, with increasing irradiating time, photodissociation occurred due to the accumulated heat and the fluorescence intensity decreased rapidly. The fast change in the fluorescence spectrum of the solid dye due to photodissociation could be measured and analyzed in real time using a static modulated Fourier transform spectrometer implemented in the laboratory. As the pumping light source, a diode laser of 1 W output power at 530 nm, in which pulse width modulation was possible, was used. When the solid-state dye sample was irradiated with a 10 Hz pulse repetition rate and 2.5 ms pulse duration for 900 s, the fluorescence intensity decreased by 44%, the fluorescence peak wavelength shifted from 590 to 586 nm, and the maximum temperature of the irradiated portion rose up to 45 °C. Under the same conditions, when the pulse duration was increased by 4 times to 10 ms, the fluorescence intensity decreased by 65%, the fluorescence peak wavelength shifted from 590 to 580 nm, and the maximum temperature of the irradiated portion rose up to 76 °C. The spectrometer proposed in this study was effective in measuring and analyzing the spectral properties of rapidly changing materials in real time.
Deformation Control of Subway Stations under the Influence of the Construction of Deep and Large Foundation Pits with Composite Support Systems
Authors: Peng Hua , Tang Qingchen , Zhu Li , Li Zichen , Li Haiyang , Wang Guangming
Published: March 2022

The excavation depth of a deep and large foundation pit of a composite support system is 25.5 m, the minimum clear distance from the main structure of the station is 10.6 m and the closest distance to the entrance and exit structure is only 3.6 m. Relying on this project, numerical simulation, field monitoring and other methods are used to study the deformation law of the self-supporting system, the ground settlement and the subway station under construction. Then, the influence of different support parameters on the deformation of the subway station and track is analyzed, and the internal relationship between the deformation value of the station and track and the support parameters is further discussed. The main work and achievements are as follows: (1) Through field measurement and analysis, the deformation history of the station and track in the excavation is studied. The obtained results show that the interbracing + pile–anchor composite support structure has better control over the deformation of adjacent stations and tracks. Comparing the simulated value of station and track deformation with the measured data, it is concluded that the simulated and measured deformation trends remain consistent, and the maximum deformation value corresponds to the middle position of the foundation pit. This confirms the rationality and reliability of the composite support system model. (2) The analysis of the influence of different support parameters on the maximum deformation value of the subway station and track shows that the deformation of the station and track is less affected by the pile diameter of the retaining pile and the size of the interbracing section. It is greatly affected by the embedding depth of the retaining pile and the number of support channels. It is suggested that the optimal parameters should be a pile diameter of 1.0 m, embedding depth of 8 m, four interbracings and an interbracing section of 1.0 m × 1.0 m.
A Rapid and Affordable Screening Tool for Early-Stage Ovarian Cancer Detection Based on MALDI-ToF MS of Blood Serum
Authors: Pais Ricardo J. , Zmuidinaite Raminta , Lacey Jonathan C. , Jardine Christian S. , Iles Ray K.
Published: March 2022

Ovarian cancer is a worldwide health issue that grows at a rate of almost 250,000 new cases every year. Its early detection is key for a good prognosis and even curative surgery. However, current medical examination methods and tests have been inefficient in detecting ovarian cancer at the early stage, leading to preventable death. So far, new screening tests based on molecular biomarker analysis techniques have not resulted in any substantial improvement in early-stage diagnosis and increased survival. Thus, whilst there remains clear potential to improve outcomes through early detection, novel approaches are needed. Here, we postulated that MALDI-ToF-mass-spectrometry-based tests can be a solution for effective screening of ovarian cancer. In this retrospective cohort study, we generated and analyzed the mass spectra of 181 serum samples of women with and without ovarian cancer. Using bioinformatics pipelines for analysis, including predictive modeling and machine learning, we found distinct mass spectral patterns composed of 9–20 key combinations of peak intensity or peak enrichment features for each stage of ovarian cancer. Based on a scoring algorithm and obtained patterns, the optimal sensitivity for detecting each stage of cancer was 95–97% with a specificity of 97%. Scoring all algorithms simultaneously could detect all stages of ovarian cancer at 99% sensitivity and 92% specificity. The results further demonstrate that the matrix and mass range analyzed played a key role in improving the mass spectral data quality and diagnostic power. Altogether, with the results reported here and increasing evidence of the MS assay’s diagnostic accuracy and instrument robustness, it has become imminent to consider MS in the clinical application for ovarian cancer screening.
A Robust Scheduling Framework for Re-Manufacturing Activities of Turbine Blades
Authors: Liu Lei , Urgo Marcello
Published: March 2022

Refurbished products are gaining importance in many industrial sectors, specifically high-value products whose residual value is relevant and guarantee the economic viability of the re-manufacturing at an industrial level, e.g., turbine blades for power generation. In this paper, we address the robust scheduling scheme of re-manufacturing activities for turbine blades. Parts entering the process may have very different wear states or presence of defects. Thus, the repair process is affected by a significant degree of uncertainty. The paper investigates the uncertainties and discusses how they affect the scheduling performance of the re-manufacturing system. We then present a robust scheduling framework for the re-manufacturing scheduling strategies, policies, and methods. This framework is based on a wide variety of experimental and practical approaches in the re-manufacturing scheduling area, which will be a guideline for the planning and scheduling of re-manufacturing activities of turbine blades. A case study approach was adopted to examine how re-manufacturers design their scheduling strategies.
The Novel Strain of Gluconobacter oxydans H32 Isolated from Kombucha as a Proposition of a Starter Culture for Sour Ale Craft Beer Production
Authors: Neffe-Skocińska Katarzyna , Kruk Marcin , Ścibisz Iwona , Zielińska Dorota
Published: March 2022

Acetic acid bacteria (AAB) has found applications in food technology, including beverages and vinegar. Generally, AAB shows several beneficial properties and has technological usefulness. Properly selected and tested strains of this group of bacteria may constitute a new and interesting solution among starter cultures for functional food. Therefore, the study aimed to develop a sour beer technology, based on the novel strain Gluconobacter oxydans H32. The microbiological, physical-chemical (HPLC method), and sensory (QDP method) quality were determined during 6 months of storage of dark and light beer samples. The AAB count at the beginning of storage was approximately 8 log CFU mL−1, and 6 log CFU mL−1 after 6 months of storage. As a result of the metabolic activity, acetic acid, gluconic acid, and ascorbic acid were detected in the samples. The light beer had a significantly better sensory quality, especially sample BPGL with the addition of G. oxydans H32 starter culture. It was found that it is possible to develop a functional beer with the novel strain Gluconobacter oxydans H32. These Sour Ale craft beers were not only a good source of H32 strain but also its pro-health metabolites.
AMPK Activation Is Indispensable for the Protective Effects of Caloric Restriction on Left Ventricular Function in Postinfarct Myocardium
Authors: Niemann Bernd , Pan Ruping , Issa Hassan , Simm Andreas , Schulz Rainer , Rohrbach Susanne
Published: March 2022

Background: Caloric restriction (CR) extends lifespan in many species, including mammals. CR is cardioprotective in senescent myocardium by correcting pre-existing mitochondrial dysfunction and apoptotic activation. Furthermore, it confers cardioprotection against acute ischemia-reperfusion injury. Here, we investigated the role of AMP-activated protein kinase (AMPK) in mediating the cardioprotective CR effects in failing, postinfarct myocardium. Methods: Ligation of the left coronary artery or sham operation was performed in rats and mice. Four weeks after surgery, left ventricular (LV) function was analyzed by echocardiography, and animals were assigned to different feeding groups (control diet or 40% CR, 8 weeks) as matched pairs. The role of AMPK was investigated with an AMPK inhibitor in rats or the use of alpha 2 AMPK knock-out mice. Results: CR resulted in a significant improvement in LV function, compared to postinfarct animals receiving control diet in both species. The improvement in LV function was accompanied by a reduction in serum BNP, decrease in LV proapoptotic activation, and increase in mitochondrial biogenesis in the LV. Inhibition or loss of AMPK prevented most of these changes. Conclusions: The failing, postischemic heart is protected from progressive loss of LV systolic function by CR. AMPK activation is indispensable for these protective effects.
Epithelial–Fibroblast Crosstalk Protects against Acidosis-Induced Inflammatory and Fibrotic Alterations
Authors: Schulz Marie-Christin , Voß Linda , Schwerdt Gerald , Gekle Michael
Published: March 2022

Pathogenesis of chronic kidney disease (CKD) is accompanied by extracellular acidosis inflammation, fibrosis and epithelial-to-mesenchymal transition (EMT). The aim of this study was to assess the influence of acidosis on tubule epithelial cells (NRK-52E) and fibroblasts (NRK-49F) in dependence of cellular crosstalk. NRK-52E and NRK-49F were used in mono- and co-cultures, and were treated with acidic media (pH 6.0) for 48 h. The intracellular proteins were measured by Western blot. Secreted proteins were measured by ELISA. Distribution of E-cadherin was assessed by immunofluorescence and epithelial barrier function by FITC-dextran diffusion. Inflammation: Acidosis led to an increase in COX-2 in NRK-52E and TNF in NRK-49F in monoculture. In co-culture, this effect was reversed. EMT: Acidosis led to an increase in vimentin protein in both cell lines, whereas in co-culture, the effect was abolished. In NRK-52E, the E-cadherin expression was unchanged, but subcellular E-cadherin showed a disturbed distribution, and cellular barrier function was decreased. Fibrosis: Monoculture acidosis led to an increased secretion of collagen I and fibronectin in NRK-52E and collagen I in NRK-49F. In co-culture, the total collagen I secretion was unchanged, and fibronectin secretion was decreased. Intercellular crosstalk between epithelial cells and fibroblasts has a protective function regarding the development of acidosis-induced damage.
Towards an Interoperable Approach for Modelling and Managing Smart Building Data: The Case of the CESI Smart Building Demonstrator
Authors: Doukari Omar , Seck Boubacar , Greenwood David , Feng Haibo , Kassem Mohamad
Published: March 2022

Buildings have a significant impact on energy consumption and carbon emissions. Smart buildings are deemed to play a crucial role in improving the energy performance of buildings and cities. Managing a smart building requires the modelling of data concerning smart systems and components. While there is a significant amount of research on optimising building energy using the smart building concept, there is a dearth of studies investigating the modelling and management of smart systems’ data, which is the starting point for establishing the necessary digital environment for representing a smart building. This study aimed to develop and test a solution for modelling and managing smart building information using an industry foundation classes (IFCs)-based BIM process. A conceptual model expressed in the SysML language was proposed to define a smart building. Five BIM approaches were identified as potential ‘prototypes’ for representing and exchanging smart building information. The fidelity of each approach is checked through a BIM-based validation process using an open-source visualisation platform. The different prototypes were also assessed using a multi-criteria comparison method to identify the preferred approach for modelling and managing smart building information. The preferred approach was prototyped and tested in a use case focused on building energy consumption monitoring to evaluate its ability to manage and visualise the smart building data. The use case was applied in a real case study using a full-scale demonstrator, namely, the ‘Nanterre 3’ (N3) smart building located at the CESI campus in Paris-Nanterre. The findings demonstrated that an open BIM format in the form of IFCs could achieve adequate modelling of smart building data without information loss. Future extensions of the proposed approach were finally outlined.
A User-Centered Evaluation of Wayfinding in Outpatient Units of Public Hospitals in Malaysia: UMMC as a Case Study
Authors: Al-Sharaa Ammar , Adam Mastura , Amer Nordin Amer Siddiq , Alhasan Ameer , Mundher Riyadh
Published: March 2022

The difficulty of finding one’s way in a complex structure has been a long-standing issue of many buildings with highly institutionalized functions. This has been especially observed in hospital buildings as an issue that can cause various adverse outcomes for both the institution and the user. Therefore, regular evaluation of the existing wayfinding system and its efficacy is needed. This study aimed to evaluate the wayfinding system efficacy in an outpatient unit of a Malaysian public hospital in order to provide information that could help guide future upgrade initiatives for existing healthcare facilities’ wayfinding systems. This study employed a user survey questionnaire to evaluate the wayfinding system currently in use and investigate users’ needs for a better wayfinding system. The statistical analysis of the gathered data indicated a higher than average level of dissatisfaction regarding the existing wayfinding system. The findings of the study suggest the need for an upgrade in the physical environment. Furthermore, the results of this study suggest the need to devise a set of guidelines to be employed when designing wayfinding systems in Malaysia’s public hospitals.
Human Close Contact Behavior-Based Interventions for COVID-19 Transmission
Authors: Miao Doudou , Zhang Nan
Published: March 2022

COVID-19 has threatened human lives. Countries have implemented various interventions such as vaccination, mask-wearing, body temperature screening, and isolation. However, the effectiveness of single and combined interventions has not yet been accurately analyzed. In this study, an improved SEIR model considering both real human indoor close contact behaviors and susceptibility to COVID-19 was established. Taking Hong Kong as an example, a quantitative assessment of the relationship between the efficiency of single and combined interventions and implementation time and intensity was carried out. The results showed that the infection risk (one-hour close contact with an infected person) of COVID-19 of students, workers, and non-workers/non-students was 3.1%, 8.7%, and 13.6%, respectively. Workplace closures were more effective among built environment interventions. If mask-wearing was mandatorily required in schools, workplaces, supermarkets, shopping centers, and public transport, COVID-19 could not be totally restricted. Workers should be prioritized for vaccination, followed by non-workers/non-students and students. Among all interventions, reducing close contact rate and increasing vaccination rate were better interventions. There was no COVID-19 outbreak (basic reproduction number R0 = 1) if the close contact reduction rate was 59.9% or the vaccination rate reached 89.5%. The results may provide scientific support for COVID-19 prevention and control.
Climate-Adaptive Façades with an Air Chamber
Authors: Vasileva Irina Leonidovna , Nemova Darya Viktorovna , Vatin Nikolai Ivanovich , Fediuk Roman Sergeevich , Karelina Maria Iurevna
Published: March 2022

The development of energy-efficient technologies at all stages of a building’s life cycle is essential to achieving sustainable development goals. The object of the study is climate-adaptive façade structures with air gaps in the form of a Trombe wall and a double-skin façade. Cases using phase-change materials (PCM) and photovoltaic modules (PV) in climate-adaptive structures are analyzed separately. The research method is aimed to review and analyze the energy-saving potential from integrating the Trombe wall or double-skin façade in buildings. The work systematizes full-scale, physical, and mathematical experiments. Articles from Scopus and Web of Science systems from 2001 to 2022 inclusive were subject to consideration. The article presents a statistical analysis given by the scientific community on the current topic’s dynamics. The study’s significance is characterized by a lack of knowledge on the behavior of the mentioned façade systems in various climate zones and for different buildings types. The results have shown that comprehensive studies on the investigated systems are significant and can serve for further designs and energy efficiency improvements. For the first time, a scientometric analysis of articles on the topic “Climate-adaptive façades” was compiled.
Advanced Nickel-Based Catalysts for Urea Oxidation Reaction: Challenges and Developments
Authors: Ma Yaming , Ma Chenxiang , Wang Yingche , Wang Ke
Published: March 2022

The electrochemical urea oxidation reaction (UOR) is crucial for determining industrial and commercial applications of urea-based energy conversion devices. However, the performance of UOR is limited by the dynamic complex of the six-electron transfer process. To this end, it is essential to develop efficient UOR catalysts. Nickel-based materials have been extensively investigated owing to their high activity, easy modification, stable properties, and cheap and abundant reserves. Various material designs and strategies have been investigated in producing highly efficient UOR catalysts including alloying, doping, heterostructure construction, defect engineering, micro functionalization, conductivity modulation, etc. It is essential to promptly review the progress in this field to significantly inspire subsequent studies. In this review, we summarized a comprehensive investigation of the mechanisms of oxidation or poisoning and UOR processes on nickel-based catalysts as well as different approaches to prepare highly active catalysts. Moreover, challenges and prospects for future developments associated with issues of UOR in urea-based energy conversion applications were also discussed.
Removal of Organics from Landfill Leachate by Heterogeneous Fenton-like Oxidation over Copper-Based Catalyst
Authors: Hussain Sajid , Aneggi Eleonora , Trovarelli Alessandro , Goi Daniele
Published: March 2022

Landfill leachates are a mixture of high concentration organic and inorganic contaminants and need to be appropriately treated due to their toxicity and severe adverse effects on the environment. Here, we studied the treatment of landfill leachate through a heterogeneous Fenton-like oxidation process using a zirconia supported copper catalyst (Cu/ZrO2). Reaction conditions such as pH, amount of catalyst, oxidant dose, temperature, and reaction time were investigated and their effects on pollutant abatement discussed. AOS (average oxidation state) and COS (carbon oxidation state) parameters were used for the evaluation of the degree of oxidation of the process, obtaining some insight into the formation of oxidized intermediates (partial oxidation) and the total oxidation (mineralization) of the leachate during the reaction. A two-step oxidation process enhanced the overall performance of the reaction with an abatement of organic compounds of 92% confirming the promising activity of a copper-based catalyst for the treatment of liquid waste. Higher catalytic activity was achieved when the following reaction conditions were applied: 70 °C, pH 5, 200 mg/L of catalyst, 30 mL/L of H2O2 dose, and 150 min. In addition, durability of the catalyst under optimized reaction conditions was verified by repeated reaction cycles.
BTK Inhibitors Impair Platelet-Mediated Antifungal Activity
Authors: Nasillo Vincenzo , Lagreca Ivana , Vallerini Daniela , Barozzi Patrizia , Riva Giovanni , Maccaferri Monica , Paolini Ambra , Forghieri Fabio , Fiorcari Stefania , Maffei Rossana , Martinelli Silvia , Atene Claudio Giacinto , Castelli Ilaria , Marasca Roberto , Potenza Leonardo , Comoli Patrizia , Manfredini Rossella , Tagliafico Enrico , Trenti Tommaso , Luppi Mario
Published: March 2022

In recent years, the introduction of new drugs targeting Bruton’s tyrosine kinase (BTK) has allowed dramatic improvement in the prognosis of patients with chronic lymphocytic leukemia (CLL) and other B-cell neoplasms. Although these small molecules were initially considered less immunosuppressive than chemoimmunotherapy, an increasing number of reports have described the occurrence of unexpected opportunistic fungal infections, in particular invasive aspergillosis (IA). BTK represents a crucial molecule in several signaling pathways depending on different immune receptors. Based on a variety of specific off-target effects on innate immunity, namely on neutrophils, monocytes, pulmonary macrophages, and nurse-like cells, ibrutinib has been proposed as a new host factor for the definition of probable invasive pulmonary mold disease. The role of platelets in the control of fungal growth, through granule-dependent mechanisms, was described in vitro almost two decades ago and is, so far, neglected by experts in the field of clinical management of IA. In the present study, we confirm the antifungal role of platelets, and we show, for the first time, that the exposure to BTK inhibitors impairs several immune functions of platelets in response to Aspergillus fumigatus, i.e., the ability to adhere to conidia, activation (as indicated by reduced expression of P-selectin), and direct killing activity. In conclusion, our experimental data suggest that antiplatelet effects of BTK inhibitors may contribute to an increased risk for IA in CLL patients.
Amelioration of Experimental Autoimmune Encephalomyelitis in Alzheimer’s Disease Mouse Models: A Potential Role for Aβ
Authors: Shi Changjie , Cha Jiaxue , Gong Junyuan , Wang Shaodeng , Zeng Peng , Lian Junjiang , Zhang Bowen , Hua Qiuhong , Lv Jie , Du Changsheng , Xie Xin , Zhang Ru
Published: March 2022

Emerging data have highlighted the coexistence of multiple sclerosis (MS) and Alzheimer’s disease (AD), both of which are common central nervous system degenerative diseases with a heavy burden on patients, their families, and society. However, it is unclear how MS progresses under an AD pathological background. We aimed to address the question of how MS progresses under an AD pathological background. We induced the experimental autoimmune encephalomyelitis (EAE) model of MS in two types of AD mouse models, Tg6799 and APP/PS1 mice. We found that, compared with wild-type mice, the clinical symptoms of EAE were significantly ameliorated in APP/PS1 mice but not in Tg6799 mice. Moreover, a much lower level of serum Aβ was observed in Tg6799 mice. EAE clinical symptoms in Tg6799 and C57BL/6J mice were ameliorated by intraperitoneal injection of Aβ42. Peripheral administration of Aβ42 peptides was able to inhibit Th17 development in vivo, which is likely to occur through the inhibition of IL-6 production in dendritic cells. Our findings revealed that AD and EAE could coexist in the same mouse, and Aβ residing in peripheral circulation likely plays an anti-inflammatory role in preventing EAE progression. These findings reveal the potential benefit of Aβ, one of the supervillains of AD, at least in certain contexts.
Cardiovascular Inflammaging: Mechanisms and Translational Aspects
Authors: Barcena Maria Luisa , Aslam Muhammad , Pozdniakova Sofya , Norman Kristina , Ladilov Yury
Published: March 2022

Aging is one of the major non-reversible risk factors for several chronic diseases, including cancer, type 2 diabetes, dementia, and cardiovascular diseases (CVD), and it is a key cause of multimorbidity, disability, and frailty (decreased physical activity, fatigue, and weight loss). The underlying cellular mechanisms are complex and consist of multifactorial processes, such as telomere shortening, chronic low-grade inflammation, oxidative stress, mitochondrial dysfunction, accumulation of senescent cells, and reduced autophagy. In this review, we focused on the molecular mechanisms and translational aspects of cardiovascular aging-related inflammation, i.e., inflammaging.
Multi-Design Differential Expression Profiling of COVID-19 Lung Autopsy Specimens Reveals Significantly Deregulated Inflammatory Pathways and SFTPC Impaired Transcription
Authors: Fassan Matteo , Collesei Antonio , Angerilli Valentina , Sbaraglia Marta , Fortarezza Francesco , Pezzuto Federica , De Gaspari Monica , Businello Gianluca , Moni Margherita , Rizzo Stefania , Traverso Giulia , Colosso Veronica , Taschin Elisa , Lunardi Francesca , Valls Aida Freire , Schiavi Francesca , Basso Cristina , Calabrese Fiorella , Dei Tos Angelo Paolo
Published: March 2022

The transcriptomic profiling of lung damage associated with SARS-CoV-2 infection may lead to the development of effective therapies to prevent COVID-19-related deaths. We selected a series of 21 autoptic lung samples, 14 of which had positive nasopharyngeal swabs for SARS-CoV-2 and a clinical diagnosis of COVID-19-related death; their pulmonary viral load was quantified with a specific probe for SARS-CoV-2. The remaining seven cases had no documented respiratory disease and were used as controls. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples was extracted to perform gene expression profiling by means of targeted (Nanostring) and comprehensive RNA-Seq. Two differential expression designs were carried out leading to relevant results in terms of deregulation. SARS-CoV-2 positive specimens presented a significant overexpression in genes of the type I interferon signaling pathway (IFIT1, OAS1, ISG15 and RSAD2), complement activation (C2 and CFB), macrophage polarization (PKM, SIGLEC1, CD163 and MS4A4A) and Cathepsin C (CTSC). CD163, Siglec-1 and Cathepsin C overexpression was validated by immunohistochemistry. SFTPC, the encoding gene for pulmonary-associated surfactant protein C, emerged as a key identifier of COVID-19 patients with high viral load. This study successfully recognized SARS-CoV-2 specific immune signatures in lung samples and highlighted new potential therapeutic targets. A better understanding of the immunopathogenic mechanisms of SARS-CoV-2 induced lung damage is required to develop effective individualized pharmacological strategies.
Influence of Target Current on Structure and Performance of Cu Films Deposited by Oscillating Pulse Magnetron Sputtering
Authors: Wang Rong , Yang Chao , Hao Juan , Shi Jing , Yan Fangyuan , Zhang Nan , Jiang Bailing , Shao Wenting
Published: March 2022

To improve the deposition rate of thin films, a novel oscillating pulse magnetron sputtering technology (OPMS) was developed to substitute the traditional high-power impulse magnetron sputtering (HiPIMS). Meanwhile, the relative density and the mechanical properties were also significantly enhanced by this method. In this study, OPMS was used to prepare the pure Cu film, and the effect of the target current on the mode of copper atoms leaving the target (off-target method) under argon gas atmosphere was also investigated. The results showed that with the increase of the target current, the off-target method of copper atoms was transformed from sputtering to evaporation, the surface cracks’ width of the deposited films gradually decreased, and the lattice constants of the Cu films were close to the bulk materials. Furthermore, the deposition rate of Cu films obviously increased from 19 to 103 nm/min. The crystal structures of Cu films showed a face-centered cubic structure, and the grain size increased from 13 to 18 nm, with the target current increased from 2 to 18 A. Moreover, Cu films deposited at currents of 8 and 13 A exhibited excellent adhesion.