bluuebunny/crossref_metadata_2025 huggingface dataset:
The Beige Adipocyte as a Therapy for Metabolic Diseases
Authors: Lizcano Fernando
Published: October 2019

Adipose tissue is traditionally categorized into white and brown relating to their function and morphology. The classical white adipose tissue builds up energy in the form of triglycerides and is useful for preventing fatigue during periods of low caloric intake and the brown adipose tissue more energetically active, with a greater number of mitochondria and energy production in the form of heat. Since adult humans possess significant amounts of active brown fat depots and its mass inversely correlates with adiposity, brown fat might play an important role in human obesity and energy homeostasis. New evidence suggests two types of thermogenic adipocytes with distinct developmental and anatomical features: classical brown adipocytes and beige adipocytes. Beige adipocyte has recently attracted special interest because of its ability to dissipate energy and the possible ability to differentiate themselves from white adipocytes. The presence of brown and beige adipocyte in human adults has acquired attention as a possible therapeutic intervention for metabolic diseases. Importantly, adult human brown appears to be mainly composed of beige-like adipocytes, making this cell type an attractive therapeutic target for obesity and obesity-related diseases, such as atherosclerosis, arterial hypertension and diabetes mellitus type 2. Because many epigenetics changes can affect beige adipocyte differentiation from adipose progenitor cells, the knowledge of the circumstances that affect the development of beige adipocyte cells may be important to new pathways in the treatment of metabolic diseases. New molecules have emerged as possible therapeutic targets, which through the impulse to develop beige adipocytes can be useful for clinical studies. In this review will discuss some recent observations arising from the unique physiological capacity of these cells and their possible role as ways to treat obesity and diabetes mellitus type 2.
Angiotensin II Influences Pre-mRNA Splicing Regulation by Enhancing RBM20 Transcription Through Activation of the MAPK/ELK1 Signaling Pathway
Authors: Cai Hanfang , Zhu Chaoqun , Chen Zhilong , Maimaiti Rexiati , Sun Mingming , McCormick Richard J. , Lan Xianyong , Chen Hong , Guo Wei
Published: October 2019

RNA binding motif 20 (RBM20) is a key regulator of pre-mRNA splicing of titin and other genes that are associated with cardiac diseases. Hormones, like insulin, triiodothyronine (T3), and angiotensin II (Ang II), can regulate gene-splicing through RBM20, but the detailed mechanism remains unclear. This study was aimed at investigating the signaling mechanism by which hormones regulate pre-mRNA splicing through RBM20. We first examined the role of RBM20 in Z-, I-, and M-band titin splicing at different ages in wild type (WT) and RBM20 knockout (KO) rats using RT-PCR; we found that RBM20 is the predominant regulator of I-band titin splicing at all ages. Then we treated rats with propylthiouracil (PTU), T3, streptozotocin (STZ), and Ang II and evaluated the impact of these hormones on the splicing of titin, LIM domain binding 3 (Ldb3), calcium/calmodulin-dependent protein kinase II gamma (Camk2g), and triadin (Trdn). We determined the activation of mitogen-activated protein kinase (MAPK) signaling in primary cardiomyocytes treated with insulin, T3, and Ang II using western blotting; MAPK signaling was activated and RBM20 expression increased after treatment. Two downstream transcriptional factors c-jun and ETS Transcription Factor (ELK1) can bind the promoter of RBM20. A dual-luciferase activity assay revealed that Ang II, but not insulin and T3, can trigger ELK1 and thus promote transcription of RBM20. This study revealed that Ang II can trigger ELK1 through activation of MAPK signaling by enhancing RBM20 expression which regulates pre-mRNA splicing. Our study provides a potential therapeutic target for the treatment of cardiac diseases in RBM20-mediated pre-mRNA splicing.
Evolutionary Analysis of JAZ Proteins in Plants: An Approach in Search of the Ancestral Sequence
Authors: Garrido-Bigotes Adrián , Valenzuela-Riffo Felipe , Figueroa Carlos
Published: October 2019

Jasmonates are phytohormones that regulate development, metabolism and immunity. Signal transduction is critical to activate jasmonate responses, but the evolution of some key regulators such as jasmonate-ZIM domain (JAZ) repressors is not clear. Here, we identified 1065 JAZ sequence proteins in 66 lower and higher plants and analyzed their evolution by bioinformatics methods. We found that the TIFY and Jas domains are highly conserved along the evolutionary scale. Furthermore, the canonical degron sequence LPIAR(R/K) of the Jas domain is conserved in lower and higher plants. It is noteworthy that degron sequences showed a large number of alternatives from gymnosperms to dicots. In addition, ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motifs are displayed in all plant lineages from liverworts to angiosperms. However, the cryptic MYC2-interacting domain (CMID) domain appeared in angiosperms for the first time. The phylogenetic analysis performed using the Maximum Likelihood method indicated that JAZ ortholog proteins are grouped according to their similarity and plant lineage. Moreover, ancestral JAZ sequences were constructed by PhyloBot software and showed specific changes in the TIFY and Jas domains during evolution from liverworts to dicots. Finally, we propose a model for the evolution of the ancestral sequences of the main eight JAZ protein subgroups. These findings contribute to the understanding of the JAZ family origin and expansion in land plants.
Effects of Encapsulated Cells on the Physical–Mechanical Properties and Microstructure of Gelatin Methacrylate Hydrogels
Authors: Krishnamoorthy Srikumar , Noorani Behnam , Xu Changxue
Published: October 2019

Gelatin methacrylate (GelMA) has been gaining popularity in recent years as a photo-crosslinkable biomaterial widely used in a variety of bioprinting and tissue engineering applications. Several studies have established the effects of process-based and material-based parameters on the physical–mechanical properties and microstructure of GelMA hydrogels. However, the effect of encapsulated cells on the physical–mechanical properties and microstructure of GelMA hydrogels has not been fully understood. In this study, 3T3 fibroblasts were encapsulated at different cell densities within the GelMA hydrogels and incubated over 96 h. The effects of encapsulated cells were investigated in terms of mechanical properties (tensile modulus and strength), physical properties (swelling and degradation), and microstructure (pore size). Cell viability was also evaluated to confirm that most cells were alive during the incubation. It was found that with an increase in cell density, the mechanical properties decreased, while the degradation and the pore size increased.
Hypoxia Induced Heparan Sulfate Primes the Extracellular Matrix for Endothelial Cell Recruitment by Facilitating VEGF-Fibronectin Interactions
Authors: Buczek-Thomas Jo Ann , Rich Celeste B. , Nugent Matthew A.
Published: October 2019

Vascular endothelial growth factor-A (VEGF) is critical for the development, growth, and survival of blood vessels. Retinal pigmented epithelial (RPE) cells are a major source of VEGF in the retina, with evidence that the extracellular matrix (ECM)-binding forms are particularly important. VEGF associates with fibronectin in the ECM to mediate distinct signals in endothelial cells that are required for full angiogenic activity. Hypoxia stimulates VEGF expression and angiogenesis; however, little is known about whether hypoxia also affects VEGF deposition within the ECM. Therefore, we investigated the role of hypoxia in modulating VEGF-ECM interactions using a primary retinal cell culture model. We found that retinal endothelial cell attachment to RPE cell layers was enhanced in cells maintained under hypoxic conditions. Furthermore, we found that agents that disrupt VEGF-fibronectin interactions inhibited endothelial cell attachment to RPE cells. We also found that hypoxia induced a general change in the chemical structure of the HS produced by the RPE cells, which correlated to changes in the deposition of VEGF in the ECM, and we further identified preferential binding of VEGFR2 over VEGFR1 to VEGF laden-fibronectin matrices. Collectively, these results indicate that hypoxia-induced HS may prime fibronectin for VEGF deposition and endothelial cell recruitment by promoting VEGF-VEGFR2 interactions as a potential means to control angiogenesis in the retina and other tissues.
Two Novel er1 Alleles Conferring Powdery Mildew (Erysiphe pisi) Resistance Identified in a Worldwide Collection of Pea (Pisum sativum L.) Germplasms
Authors: Sun Suli , Deng Dong , Duan Canxing , Zong Xuxiao , Xu Dongxu , He Yuhua , Zhu Zhendong
Published: October 2019

Powdery mildew caused by Erysiphe pisi DC. severely affects pea crops worldwide. The use of resistant cultivars containing the er1 gene is the most effective way to control this disease. The objectives of this study were to reveal er1 alleles contained in 55 E. pisi-resistant pea germplasms and to develop the functional markers of novel alleles. Sequences of 10 homologous PsMLO1 cDNA clones from each germplasm accession were used to determine their er1 alleles. The frame shift mutations and various alternative splicing patterns were observed during transcription of the er1 gene. Two novel er1 alleles, er1-8 and er1-9, were discovered in the germplasm accessions G0004839 and G0004400, respectively, and four known er1 alleles were identified in 53 other accessions. One mutation in G0004839 was characterized by a 3-bp (GTG) deletion of the wild-type PsMLO1 cDNA, resulting in a missing valine at position 447 of the PsMLO1 protein sequence. Another mutation in G0004400 was caused by a 1-bp (T) deletion of the wild-type PsMLO1 cDNA sequence, resulting in a serine to leucine change of the PsMLO1 protein sequence. The er1-8 and er1-9 alleles were verified using resistance inheritance analysis and genetic mapping with respectively derived F2 and F2:3 populations. Finally, co-dominant functional markers specific to er1-8 and er1-9 were developed and validated in populations and pea germplasms. These results improve our understanding of E. pisi resistance in pea germplasms worldwide and provide powerful tools for marker-assisted selection in pea breeding.
Mechanical Ventilation Impairs IL-17 Cytokine Family Expression in Ventilator-Associated Pneumonia
Authors: De Winter Fien , ’s Jongers Bart , Bielen Kenny , Mancuso Domenico , Timbermont Leen , Lammens Christine , Van averbeke Vincent , Boddaert Jan , Ali Omar , Kluytmans Jan , Ruzin Alexey , Malhotra-Kumar Surbhi , Jorens Philippe , Goossens Herman , Kumar-Singh Samir
Published: October 2019

Mechanical ventilation (MV) is the primary risk factor for the development of ventilator-associated pneumonia (VAP). Besides inducing a pro-inflammatory T-helper (Th)-1 cytokine response, MV also induces an anti-inflammatory Th2 cytokine response, marked by increased IL-4 secretion and reduced bacterial phagocytic capacity of rodent lung macrophages. Since IL-4 is known to downregulate both Th1 and Th17 cytokines, the latter is important in mediating mucosal immunity and combating bacterial and fungal growth, we studied and showed here in a rat model of MV that Th17 cytokines (IL-17A, IL-17F, and IL-22) were significantly upregulated in the lung as a response to different MV strategies currently utilized in clinic. To study whether the increased IL-4 levels are associated with downregulation of the anti-bacterial Th17 cytokines, we subsequently challenged mechanically ventilated rats with an intratracheal inoculation of Pseudomonas aeruginosa (VAP model) and showed a dramatic downregulation of IL-17A, IL-17F, and IL-22, compared to animals receiving the same bacterial burden without MV. For the studied Th1 cytokines (IFNγ, TNFα, IL-6, and IL-1β), only IFNγ showed a significant decrease as a consequence of bacterial infection in mechanically ventilated rats. We further studied IL-17A, the most studied IL-17 family member, in intensive care unit (ICU) pneumonia patients and showed that VAP patients had significantly lower levels of IL-17A in the endotracheal aspirate compared to patients entering ICU with pre-existing pneumonia. These translational data, obtained both in animal models and in humans, suggest that a deficient anti-bacterial Th17 response in the lung during MV is associated with VAP development.
Graphene Oxide Enhances Chitosan-Based 3D Scaffold Properties for Bone Tissue Engineering
Authors: Dinescu Sorina , Ionita Mariana , Ignat Simona-Rebeca , Costache Marieta , Hermenean Anca
Published: October 2019

The main goal of bone tissue engineering (BTE) is to refine and repair major bone defects based on bioactive biomaterials with distinct properties that can induce and support bone tissue formation. Graphene and its derivatives, such as graphene oxide (GO), display optimal properties for BTE, being able to support cell growth and proliferation, cell attachment, and cytoskeleton development as well as the activation of osteogenesis and bone development pathways. Conversely, the presence of GO within a polymer matrix produces favorable changes to scaffold morphologies that facilitate cell attachment and migration i.e., more ordered morphologies, greater surface area, and higher total porosity. Therefore, there is a need to explore the potential of GO for tissue engineering applications and regenerative medicine. Here, we aim to promote one novel scaffold based on a natural compound of chitosan, improved with 3 wt.% GO, for BTE approaches, considering its good biocompatibility, remarkable 3D characteristics, and ability to support stem cell differentiation processes towards the bone lineage.
The Effect of Long-Term Use of an Eyewash Solution on the Ocular Surface Mucin Layer
Authors: Yazu Hiroyuki , Kozuki Naoyuki , Dogru Murat , Shibasaki Ayako , Fujishima Hiroshi
Published: October 2019

The use of eyewash solutions in Japan, especially in patients with allergic conjunctivitis and contact lens wearers, has been increasing. Our aim was to investigate how the use of preservative-free eyewash solution in healthy eyes for one month affects corneal safety and ocular surface mucin. We analyzed 42 eyes of 21 individuals (17 males, four females; mean age: 36.1 ± 7.4 years) without ocular allergies, dry eyes, or other ocular diseases through a prospective study. Eyes were randomized to a wash group (group one) and a nonwash follow up group (group two). We evaluated the dry eye-related quality-of-life score (DEQS), tear film breakup time (TBUT), fluorescein staining score, mRNA expression of MUC5AC and MUC16, MUC16 immunohistochemistry, and MUC5AC periodic acid Schiff (PAS) staining. There was a significant decrease in DEQS scores after one month of eyewash use (p < 0.05). There were no significant differences in other evaluation items that were analyzed (all p > 0.05). Furthermore, no significant differences were observed between group one and group two in all endpoints (all p > 0.05). The results suggest that one month use of a nonpreserved eyewash solution has no detrimental effects on the tear film and the ocular surface mucins.
Proteomic Landscape Has Revealed Small Rubber Particles Are Crucial Rubber Biosynthetic Machines for Ethylene-Stimulation in Natural Rubber Production
Authors: Wang Dan , Xie Quanliang , Sun Yong , Tong Zheng , Chang Lili , Yu Li , Zhang Xueyan , Yuan Boxuan , He Peng , Jin Xiang , Dong Yiyang , Li Hongbin , Montoro Pascal , Wang Xuchu
Published: October 2019

Rubber particles are a specific organelle for natural rubber biosynthesis (NRB) and storage. Ethylene can significantly improve rubber latex production by increasing the generation of small rubber particles (SRPs), regulating protein accumulation, and activating many enzyme activities. We conducted a quantitative proteomics study of different SRPs upon ethylene stimulation by differential in-gel electrophoresis (DIGE) and using isobaric tags for relative and absolute quantification (iTRAQ) methods. In DIGE, 79 differentially accumulated proteins (DAPs) were determined as ethylene responsive proteins. Our results show that the abundance of many NRB-related proteins has been sharply induced upon ethylene stimulation. Among them, 23 proteins were identified as rubber elongation factor (REF) and small rubber particle protein (SRPP) family members, including 16 REF and 7 SRPP isoforms. Then, 138 unique phosphorylated peptides, containing 129 phosphorylated amino acids from the 64 REF/SRPP family members, were identified, and most serine and threonine were phosphorylated. Furthermore, we identified 226 DAPs from more than 2000 SRP proteins by iTRAQ. Integrative analysis revealed that almost all NRB-related proteins can be detected in SRPs, and many proteins are positively responsive to ethylene stimulation. These results indicate that ethylene may stimulate latex production by regulating the accumulation of some key proteins. The phosphorylation modification of REF and SRPP isoforms might be crucial for NRB, and SRP may act as a complex natural rubber biosynthetic machine.
Crystal Structure-Based Exploration of Arginine-Containing Peptide Binding in the ADP-Ribosyltransferase Domain of the Type III Effector XopAI Protein
Authors: Liu Jyung-Hurng , Yang Jun-Yi , Hsu Duen-Wei , Lai Yi-Hua , Li Yun-Pei , Tsai Yi-Rung , Hou Ming-Hon
Published: October 2019

Plant pathogens secrete proteins called effectors into the cells of their host to modulate the host immune response against colonization. Effectors can either modify or arrest host target proteins to sabotage the signaling pathway, and therefore are considered potential drug targets for crop disease control. In earlier research, the Xanthomonas type III effector XopAI was predicted to be a member of the arginine-specific mono-ADP-ribosyltransferase family. However, the crystal structure of XopAI revealed an altered active site that is unsuitable to bind the cofactor NAD+, but with the capability to capture an arginine-containing peptide from XopAI itself. The arginine peptide consists of residues 60 through 69 of XopAI, and residue 62 (R62) is key to determining the protein–peptide interaction. The crystal structure and the molecular dynamics simulation results indicate that specific arginine recognition is mediated by hydrogen bonds provided by the backbone oxygen atoms from residues W154, T155, and T156, and a salt bridge provided by the E265 sidechain. In addition, a protruding loop of XopAI adopts dynamic conformations in response to arginine peptide binding and is probably involved in target protein recognition. These data suggest that XopAI binds to its target protein by the peptide-binding ability, and therefore, it promotes disease progression. Our findings reveal an unexpected and intriguing function of XopAI and pave the way for further investigation on the role of XopAI in pathogen invasion.
The G9a Histone Methyltransferase Inhibitor BIX-01294 Modulates Gene Expression during Plasmodium falciparum Gametocyte Development and Transmission
Authors: Ngwa Che Julius , Kiesow Meike Jutta , Orchard Lindsey Marie , Farrukh Afia , Llinás Manuel , Pradel Gabriele
Published: October 2019

Transmission of the malaria parasite Plasmodium falciparum from the human to the mosquito is initiated by specialized sexual cells, the gametocytes. In the human, gametocytes are formed in response to stress signals and following uptake by a blood-feeding Anopheles mosquito initiate sexual reproduction. Gametocytes need to fine-tune their gene expression in order to develop inside the mosquito to continue life-cycle progression. Previously, we showed that post-translational histone acetylation controls gene expression during gametocyte development and transmission. However, the role of histone methylation remains poorly understood. We here use the histone G9a methyltransferase inhibitor BIX-01294 to investigate the role of histone methylation in regulating gene expression in gametocytes. In vitro assays demonstrated that BIX-01294 inhibits intraerythrocytic replication with a half maximal inhibitory concentration (IC50) of 13.0 nM. Furthermore, BIX-01294 significantly impairs gametocyte maturation and reduces the formation of gametes and zygotes. Comparative transcriptomics between BIX-01294-treated and untreated immature, mature and activated gametocytes demonstrated greater than 1.5-fold deregulation of approximately 359 genes. The majority of these genes are transcriptionally downregulated in the activated gametocytes and could be assigned to transcription, translation, and signaling, indicating a contribution of histone methylations in mediating gametogenesis. Our combined data show that inhibitors of histone methylation may serve as a multi-stage antimalarial.
Dietary Polyphenols: A Multifactorial Strategy to Target Alzheimer’s Disease
Authors: Dhakal Sudip , Kushairi Naufal , Phan Chia Wei , Adhikari Benu , Sabaratnam Vikineswary , Macreadie Ian
Published: October 2019

Ageing is an inevitable fundamental process for people and is their greatest risk factor for neurodegenerative disease. The ageing processes bring changes in cells that can drive the organisms to experience loss of nutrient sensing, disrupted cellular functions, increased oxidative stress, loss of cellular homeostasis, genomic instability, accumulation of misfolded protein, impaired cellular defenses and telomere shortening. Perturbation of these vital cellular processes in neuronal cells can lead to life threatening neurological disorders like Alzheimer’s Disease, Parkinson’s Disease, Huntington’s Disease, Lewy body dementia, etc. Alzheimer’s Disease is the most frequent cause of deaths in the elderly population. Various therapeutic molecules have been designed to overcome the social, economic and health care burden caused by Alzheimer’s Disease. Almost all the chemical compounds in clinical practice have been found to treat symptoms only limiting them to palliative care. The reason behind such imperfect drugs may result from the inefficiencies of the current drugs to target the cause of the disease. Here, we review the potential role of antioxidant polyphenolic compounds that could possibly be the most effective preventative strategy against Alzheimer’s Disease.
Epigenetics, Stem Cells, and Autophagy: Exploring a Path Involving miRNA
Authors: Balzano Francesca , Campesi Ilaria , Cruciani Sara , Garroni Giuseppe , Bellu Emanuela , Dei Giudici Silvia , Angius Andrea , Oggiano Annalisa , Rallo Vincenzo , Capobianco Giampiero , Dessole Salvatore , Ventura Carlo , Montella Andrea , Maioli Margherita
Published: October 2019

MiRNAs, a small family of non-coding RNA, are now emerging as regulators of stem cell pluripotency, differentiation, and autophagy, thus controlling stem cell behavior. Stem cells are undifferentiated elements capable to acquire specific phenotype under different kind of stimuli, being a main tool for regenerative medicine. Within this context, we have previously shown that stem cells isolated from Wharton jelly multipotent stem cells (WJ-MSCs) exhibit gender differences in the expression of the stemness related gene OCT4 and the epigenetic modulator gene DNA-Methyltransferase (DNMT1). Here, we further analyze this gender difference, evaluating adipogenic and osteogenic differentiation potential, autophagic process, and expression of miR-145, miR-148a, and miR-185 in WJ-MSCs derived from males and females. These miRNAs were selected since they are involved in OCT4 and DNMT1 gene expression, and in stem cell differentiation. Our results indicate a difference in the regulatory circuit involving miR-148a/DNMT1/OCT4 autophagy in male WJ-MSCs as compared to female cells. Moreover, no difference was detected in the expression of the two-differentiation regulating miRNA (miR-145 and miR-185). Taken together, our results highlight a different behavior of WJ-MSCs from males and females, disclosing the chance to better understand cellular processes as autophagy and stemness, usable for future clinical applications.
Advances in the Mechanisms of Plant Tolerance to Manganese Toxicity
Authors: Li Jifu , Jia Yidan , Dong Rongshu , Huang Rui , Liu Pandao , Li Xinyong , Wang Zhiyong , Liu Guodao , Chen Zhijian
Published: October 2019

Manganese (Mn) is an essential element for plant growth due to its participation in a series of physiological and metabolic processes. Mn is also considered a heavy metal that causes phytotoxicity when present in excess, disrupting photosynthesis and enzyme activity in plants. Thus, Mn toxicity is a major constraint limiting plant growth and production, especially in acid soils. To cope with Mn toxicity, plants have evolved a wide range of adaptive strategies to improve their growth under this stress. Mn tolerance mechanisms include activation of the antioxidant system, regulation of Mn uptake and homeostasis, and compartmentalization of Mn into subcellular compartments (e.g., vacuoles, endoplasmic reticulum, Golgi apparatus, and cell walls). In this regard, numerous genes are involved in specific pathways controlling Mn detoxification. Here, we summarize the recent advances in the mechanisms of Mn toxicity tolerance in plants and highlight the roles of genes responsible for Mn uptake, translocation, and distribution, contributing to Mn detoxification. We hope this review will provide a comprehensive understanding of the adaptive strategies of plants to Mn toxicity through gene regulation, which will aid in breeding crop varieties with Mn tolerance via genetic improvement approaches, enhancing the yield and quality of crops.
A Study on Cyber Security Threats in a Shipboard Integrated Navigational System
Authors: Svilicic Boris , Rudan Igor , Jugović Alen , Zec Damir
Published: October 2019

The integrated navigational system (INS) enhances the effectiveness and safety of ship navigation by providing multifunctional display on the basis of integration of at least two navigational functions, the voyage route monitoring with Electronic Chart Display and Information System (ECDIS) and collision avoidance with radar. The INS is essentially a software platform for fusion of data from the major ECDIS and radar systems with sensors for the additional navigation functions of route planning, status and data display, and alert management. This paper presents a study on cyber security resilience examination of a shipboard INS installed on a RoPax ship engaged in international trade. The study was based on a mixed-method approach, combining an interview of the ship’s navigational ranks and cyber security testing of the INS using an industry vulnerability scanner. The identified threats were analyzed qualitatively to study the source of cyber risks threatening the INS. The results obtained point out cyber threats related to weaknesses of the INS underlying operating system, suggesting a need for occasional preventive maintenance in addition to the regulatory compliance required.
Farmers Willingness to Participate In Voluntary Land Consolidation in Gozamin District, Ethiopia
Authors: Gedefaw Abebaw Andarge , Atzberger Clement , Seher Walter , Mansberger Reinfried
Published: October 2019

In many African countries and especially in the highlands of Ethiopia—the investigation site of this paper—agricultural land is highly fragmented. Small and scattered parcels impede a necessary increase in agricultural efficiency. Land consolidation is a proper tool to solve inefficiencies in agricultural production, as it enables consolidating plots based on the consent of landholders. Its major benefits are that individual farms get larger, more compact, contiguous parcels, resulting in lower cultivation efforts. This paper investigates the determinants influencing the willingness of landholder farmers to participate in voluntary land consolidation processes. The study was conducted in Gozamin District, Amhara Region, Ethiopia. The study was mainly based on survey data collected from 343 randomly selected landholder farmers. In addition, structured interviews and focus group discussions with farmers were held. The collected data were analyzed quantitatively mainly by using a logistic regression model and qualitatively by using focus group discussions and expert panels. According to the results, landholder farmers are predominantly willing to participate in voluntary land consolidation (66.8%), while a substantive fraction of farmers express unease with voluntary land consolidation. The study highlighted the following four determinants to be significant in influencing the willingness of farmers for voluntary land consolidation: (1) the exchange should preferably happen with parcels of neighbors, (2) land consolidation should lead to better arranged parcels, (3) nearness of plots to the farmstead, and (4) an expected improvement in productivity. Interestingly, the majority of farmers believes that land consolidation could reduce land use conflicts. The study provides evidence that policymakers should consider these socio-economic, legal, cultural, infrastructural, and land-related factors when designing and implementing voluntary land consolidation policies and programs.
Biological Assessment of Zn–Based Absorbable Metals for Ureteral Stent Applications
Authors: Paramitha Devi , Chabaud Stéphane , Bolduc Stéphane , Hermawan Hendra
Published: October 2019

The use of ureteral stents to relieve urinary tract obstruction is still challenged by the problems of infection, encrustation, and compression, leading to the need for early removal procedures. Biodegradable ureteral stents, commonly made of polymers, have been proposed to overcome these problems. Recently, absorbable metals have been considered as potential materials offering both biodegradation and strength. This work proposed zinc-based absorbable metals by firstly evaluating their cytocompatibility toward normal primary human urothelial cells using 2D and 3D assays. In the 2D assay, the cells were exposed to different concentrations of metal extracts (i.e., 10 mg/mL of Zn–1Mg and 8.75 mg/mL of Zn–0.5Al) for up to 3 days and found that their cytoskeletal networks were affected but were recovered at day 3, as observed by immunofluorescence. In the 3D ureteral wall tissue construct, the cells formed a multilayered urothelium, as found in native tissue, with the presence of tight junctions at the superficial layer and laminin at the basal layer, indicating a healthy tissue condition even with the presence of the metal samples for up to 7 days of exposure. The basal cells attached to the metal surface as seen in a natural spreading state with pseudopodia and fusiform morphologies, indicating that the metals were non-toxic.
Tensile Behavior Characteristics of High-Performance Slurry-Infiltrated Fiber-Reinforced Cementitious Composite with Respect to Fiber Volume Fraction
Authors: Kim Seungwon , Kim Dong Joo , Kim Sung-Wook , Park Cheolwoo
Published: October 2019

Concrete has high compressive strength, but low tensile strength, bending strength, toughness, low resistance to cracking, and brittle fracture characteristics. To overcome these problems, fiber-reinforced concrete, in which the strength of concrete is improved by inserting fibers, is being used. Recently, high-performance fiber-reinforced cementitious composites (HPFRCCs) have been extensively researched. The disadvantages of conventional concrete such as low tensile stress, strain capacity, and energy absorption capacity, have been overcome using HPFRCCs, but they have a weakness in that the fiber reinforcement has only 2% fiber volume fraction. In this study, slurry infiltrated fiber reinforced cementitious composites (SIFRCCs), which can maximize the fiber volume fraction (up to 8%), was developed, and an experimental study on the tensile behavior of SIFRCCs with varying fiber volume fractions (4%, 5%, and 6%) was carried out through direct tensile tests. The results showed that the specimen with high fiber volume fraction exhibited high direct tensile strength and improved brittleness. As per the results, the direct tensile strength is approximately 15.5 MPa, and the energy absorption capacity was excellent. Furthermore, the bridging effect of steel fibers induced strain hardening behavior and multiple cracks, which increased the direct tensile strength and energy absorption capacity.
Strength Development and Thermogravimetric Investigation of High-Volume Fly Ash Binders
Authors: Zhou Zhiyuan , Sofi Massoud , Lumantarna Elisa , San Nicolas Rackel , Hadi Kusuma Gideon , Mendis Priyan
Published: October 2019

To address sustainability issues by facilitating the use of high-volume fly ash (HVFA) concrete in industry, this paper investigates the early age hydration properties of HVFA binders in concrete and the correlation between hydration properties and compressive strengths of the cement pastes. A new method of calculating the chemically bound water of HVFA binders was used and validated. Fly ash (FA) types used in this study were sourced from Indonesia and Australia for comparison. The water to binder (w/b) ratio was 0.4 and FA replacement levels were 40%, 50% and 60% by weight. Isothermal calorimetry tests were conducted to study the heat of hydration which was further converted to the adiabatic temperature rise. Thermo-gravimetric analysis (TGA) was employed to explore the chemically bound water (WB) of the binders. The results showed that Australian FA pastes had higher heat of hydration, adiabatic temperature rise, WB and compressive strength compared to Indonesian FA pastes. The new method of calculating chemically bound water can be successfully applied to HVFA binders. Linear correlation could be found between the WB and compressive strength.
Application of the Ansys Electronics Desktop Software Package for Analysis of Claw-Pole Synchronous Motor
Authors: Gandzha Sergey , Kosimov Bakhtiyor , Aminov Dilshod
Published: October 2019

The article presents the method of synthesis and analysis of the brushless motor with claw-poles. The motor is designed for the pilger mill drive for manufacturing seamless tubes. The peculiarity of the drive is that electric machines of this class for high power have not yet been used in world practice. In this regard, a very thorough analysis of the designed motor is required to remove technical risks in the manufacture of prototypes. For design of this motor the synthesis system and the analysis system were developed. The synthesis system is developed on the basis of nonlinear programming methods. For the analysis, it is proposed to use the program Ansys Electronics Desktop, which implements the finite element method. The peculiarity of using this program is the need to use large computer resources. Herewith, the calculation time of the main characteristics is often not acceptable for practical calculations and takes several hours. The authors propose a simplification of the computational model without significantly reducing the accuracy of the calculation. The brushless motor with claw-poles is replaced by a brushless motor with tangential magnets. The stator designs and the magnetic fluxes of the motors are the same. The effectiveness of such a replacement is shown in the real project. Calculation time with acceptable quality is reduced to a few minutes. This approach is recommended for the creation of design systems of other types of brushless machines. This scientific research was carried out for a real customer.
Extensive Tandem Duplication Events Drive the Expansion of the C1q-Domain-Containing Gene Family in Bivalves
Authors: Gerdol Marco , Greco Samuele , Pallavicini Alberto
Published: October 2019

C1q-domain-containing (C1qDC) proteins are rapidly emerging as key players in the innate immune response of bivalve mollusks. Growing experimental evidence suggests that these highly abundant secretory proteins are involved in the recognition of microbe-associated molecular patterns, serving as lectin-like molecules in the bivalve proto-complement system. While a large amount of functional data concerning the binding specificity of the globular head C1q domain and on the regulation of these molecules in response to infection are quickly accumulating, the genetic mechanisms that have led to the extraordinary lineage-specific expansion of the C1qDC gene family in bivalves are still largely unknown. The analysis of the chromosome-scale genome assembly of the Eastern oyster Crassostrea virginica revealed that the 476 oyster C1qDC genes, far from being uniformly distributed along the genome, are located in large clusters of tandemly duplicated paralogs, mostly found on chromosomes 7 and 8. Our observations point out that the evolutionary process behind the development of a large arsenal of C1qDC lectin-like molecules in marine bivalves is still ongoing and likely based on an unequal crossing over.
Epidemiology of Heart Disease of Uncertain Etiology: A Population Study and Review of the Problem
Authors: Menotti Alessandro , Puddu Paolo Emilio
Published: October 2019

Background and objectives: Previous epidemiological studies have identified a group of heart diseases (here called heart diseases of uncertain etiology—HDUE) whose characteristics were rather different from cases classified as coronary heart disease (CHD), but frequently confused with them. This analysis had the purpose of adding further evidence on this issue based on a large population study. Materials and Methods: Forty-five Italian population samples for a total of 25,272 men and 21,895 women, free from cardiovascular diseases, were examined with measurement of some risk factors. During follow-up, CHD deaths were those manifested as myocardial infarction, other acute ischemic attacks, and sudden death of probable coronary origin, after reasonable exclusion of other causes. Cases of HDUE were those manifested only as heart failure, chronic arrhythmia, and blocks in the absence of typical coronary syndromes. Cox proportional hazards models were computed separately for CHD and HDUE, with 11 risk factors as possible predictors. Results: During an average of 7.4 years (extremes 1–16) there were 223 CHD and 150 HDUE fatal events. Male sex, age, smoking habits, systolic blood pressure, serum cholesterol, and plasma glucose were significantly and directly related to CHD events, while high density lipoprotein (HDL) cholesterol was so in an inverse way. The same risk factors were predictive of HDUE events except serum cholesterol and HDL cholesterol. Multivariable hazards ratio of serum cholesterol (delta = 1 mmol/L) was higher in the CHD model (1.24, 95% CI 1.11–1.39) than in the HDUE model (1.03, 0.5% C.I. 0.89–1.19) and the difference between the respective coefficients was statistically significant (p = 0.0444). Age at death was not different between the two end-points. Conclusions: CHD and HDUE are probably two different morbid conditions, only the first one is likely bound to gross atherosclerotic lesions of coronary arteries and linked to blood lipid levels. We reviewed the problem in epidemiological investigations and addressed inflammation as a potential cofactor to differentiate between CHD and HDUE.
Screen Use During Meals Among Young Children: Exploration of Associated Variables
Authors: Jusienė Roma , Urbonas Vaidotas , Laurinaitytė Ilona , Rakickienė Lauryna , Breidokienė Rima , Kuzminskaitė Monika , Praninskienė Rūta
Published: October 2019

Background and Objectives: There is evidence that eating meals or snacks while watching TV is an obesogenic factor. Moreover, the patterns of TV and other screen use during meals begin early and persist. However, there are only a few studies to date which address the prevalence and predictors of young children’s exposure to screen during mealtimes. Thus, the present study aimed to investigate the prevalence and the associated factors of screen use during meals in early childhood. Materials and Methods: A cross sectional survey was conducted in Lithuania. Data of 847 children aged 2 to 5 years old (51.5% boys) were analyzed in this study. Parents completed the Child Behavior Checklist (CBCL/1½-5) and reported their children’s daily screen time, exposure to background TV, screen use during child’s meals, child and parental height and weight, and sociodemographic data. Results: More than half of children were exposed to screen during meals: 33.7% occasionally, several times per week or per month, and 22%—daily or during every meal. Overall daily screen time, background TV, consumption of junk food, child age, and emotional and behavioral problems were related to mealtime screen use (all associations significant at p < 0.01). Longer daily screen time (OR 1.01; 95% CI 1.00–1.01), more background TV (OR 1.26; 95% CI 1.10–1.45), and elder child age (OR 1.02; 95% CI 1.00–1.03) were significant predictors of occasional use of screen during meals. Also, longer daily screen time (OR 0.99; 95% CI 0.98–0.99), background TV (OR 0.78; 95% CI 0.66–0.91) together with no siblings’ status of a child (OR 0.42; 95% CI 0.25–0.69) increased the probability that children were fed in front of screens daily. Conclusions: This study confirmed the unfavorable associations among screen use during meals, daily screen time and junk food consumption in early childhood. In addition, first-time parents should get particular health providers’ attention as they are more likely to use screens during child’s mealtime.
Phylogenetic Analysis of Lednice Orthobunyavirus
Authors: Berčič Rebeka Lucijana , Bányai Krisztián , Růžek Daniel , Fehér Enikő , Domán Marianna , Danielová Vlasta , Bakonyi Tamás , Nowotny Norbert
Published: October 2019

Lednice virus (LEDV) has been detected in Culex modestus mosquitoes in several European countries within the last six decades. In this study, phylogenetic analyses of the complete genome segments confirm that LEDV belongs to the Turlock orthobunyavirus (Orthobunyavirus, Peribunyaviridae) species and is closely related to Umbre, Turlock, and Kedah viruses.
Cu(II)-Catalyzed Homocouplings of (Hetero)Arylboronic Acids with the Assistance of 2-O-Methyl-d-Glucopyranose
Authors: Yuan Chunling , Zheng Li , Zhao Yingdai
Published: October 2019

This is the first report of a natural ligand improving the copper-catalyzed homocouplings of (hetero)arylboronic acids. Various important synthetic biaryl intermediates in organic synthesis could be assembled via this method. To gain insight into this reaction, in situ React IR technology was used to confirm the effectivity of this catalyst system. This protocol could provide important biaryl compounds in high yields within a short time.
Differential Effects of Typical Korean Versus American-Style Diets on Gut Microbial Composition and Metabolic Profile in Healthy Overweight Koreans: A Randomized Crossover Trial
Authors: Shin Ji-Hee , Jung Sunhee , Kim Seong-Ah , Kang Min-Sook , Kim Min-Sun , Joung Hyojee , Hwang Geum-Sook , Shin Dong-Mi
Published: October 2019

The Westernized diet has been associated with the pathogenesis of metabolic diseases, whereas a Korean diet has been reported to exert beneficial effects on health in several studies. However, the effects of Western and Korean diets on the gut microbiome and host metabolome are unclear. To examine the diet-specific effects on microbiome and metabolome, we conducted a randomized crossover clinical trial of typical Korean diet (TKD), typical American diet (TAD), and recommended American diet (RAD). The trial involved a 4-week consumption of an experimental diet followed by a 2-week interval before diet crossover. 16S rRNA sequencing analysis identified 16, 10, and 14 differential bacteria genera specific to TKD, RAD, and TAD, respectively. The Firmucutes-Bacteroidetes ratio was increased by TKD. Nuclear magnetic resonance metabolome profiling revealed that TKD enriched branched chain amino acid metabolism, whereas ketone body metabolism was evident in RAD and TAD. Microbiome and metabolome responses to the experimental diets varied with individual enterotypes. These findings provide evidence that the gut microbiome and host metabolome rapidly respond to different cultural diets. The findings will inform clarification of the diet-related communication networks of the gut microbiome and host metabolome in humans.
Sodium Intake from Foods Exceeds Recommended Limits in the Spanish Population: The ANIBES Study
Authors: Partearroyo Teresa , Samaniego-Vaesken Mª de Lourdes , Ruiz Emma , Aranceta-Bartrina Javier , Gil Ángel , González-Gross Marcela , Ortega Rosa M. , Serra-Majem Lluis , Varela-Moreiras Gregorio
Published: October 2019

Excessive sodium consumption is associated with adverse health effects. An elevated dietary intake of salt (sodium chloride) has been related to high blood pressure or hypertension, a major but modifiable risk factor for cardiovascular disease, as well as to other ill health conditions. In the present work, our aim was to describe the contribution of foods to sodium consumption within the Spanish population in a representative sample from the “anthropometric data, macronutrients and micronutrients intake, practice of physical activity, socioeconomic data and lifestyles in Spain” (ANIBES) study (9–75 years), to identify high consumer groups, as well as the major food groups that contribute to sodium intake in the Spanish diet. Intakes were assessed by 3-day food records collected on a tablet device. Sodium intakes across the ANIBES study population exceeded recommendations, as total intakes reached 2025 ± 805 mg of sodium per day, that is approximately 5.06 g/day of salt (excluding discretionary salt, added at the table or during cooking). Sodium intakes were higher in males than in females and within the youngest groups. Main dietary sources of sodium were meat and meat products (27%), cereals and grains (26%), milk and dairy products (14%) and ready-to-eat meals (13%). Given the established health benefits of dietary salt reduction, it would be advisable to continue and even improve the current national initiatives of awareness and educational campaigns and particularly food reformulation to decrease overall salt intakes across the Spanish population.
Polymer Blends for Improved CO2 Capture Membranes
Authors: Zare Alireza , Perna Lorenza , Nogalska Adrianna , Ambrogi Veronica , Cerruti Pierfrancesco , Tylkowski Bartosz , García-Valls Ricard , Giamberini Marta
Published: October 2019

We investigated the possibility of improving the performance of polysulfone (PSf) membranes to be used in carbon dioxide capture devices by blending PSf with a commercial polyethylene imine, Lupasol G20, previously modified with benzoyl chloride (mG20). Additive amount ranged between 2 and 20 wt %. Membranes based on these blends were prepared by phase inversion precipitation and exhibited different morphologies with respect to neat PSf. Surface roughness, water contact angles, and water uptake increased with mG20 content. Mass transfer coefficient was also increased for both N2 and CO2; however, this effect was more evident for carbon dioxide. Carbon dioxide absorption performance of composite membranes was evaluated for potassium hydroxide solution in a flat sheet membrane contactor (FSMC) in cross flow module at different liquid flow rates. We found that, at the lowest flow rate, membranes exhibit a very similar behaviour to neat PSf; nevertheless, significant differences can be found at higher flow rates. In particular, the membranes with 2 and 5 wt % additive behave more efficiently than neat PSf. In contrast, 10 and 20 wt % additive content has an adverse effect on CO2 capture when compared with neat PSf. In the former case, a combination of additive chemical affinity to CO2 and membrane porosity can be claimed; in the latter case, the remarkably higher wettability and water uptake could determine membrane clogging and consequent loss of efficiency in the capture device.