bluuebunny/crossref_metadata_2025 huggingface dataset:
Functional Evaluation of Splicing for Variants of Uncertain Significance in Patients with Inherited Retinal Diseases
Authors: Mauro-Herrera Margarita , Chiang John , Radojevic Bojana , Bennett Lea D
Published: June 2021

Inherited retinal diseases (IRD) comprise a heterogeneous set of clinical and genetic disorders that lead to blindness. Given the emerging opportunities in precision medicine and gene therapy, it has become increasingly important to determine whether DNA variants with uncertain significance (VUS) are responsible for patients’ IRD. This research was performed to assess the functional consequence of six VUS identified in patients with IRD. Clinical assessments included an ophthalmic examination, best-corrected visual acuity, and kinetic perimetry. Imaging was acquired with the Optos ultra-widefield camera and spectral domain optical coherence tomography (SD-OCT). Genetic testing was performed by Molecular Vision Laboratories. VUS that were predicted to alter splicing were analyzed with a minigene assay, which revealed that VUS in the genes OPA1, CNGB1, and CLUAP1 altered spicing mechanisms. Due to emerging gene and cell therapies, these results expand the genotype-phenotype correlations for patients diagnosed with an IRD.
Abnormal Long Non-Coding RNAs Expression Patterns Have the Potential Ability for Predicting Survival and Treatment Response in Breast Cancer
Authors: Pavanelli Ana Carolina , Mangone Flavia Rotea , Barros Luciana R. C. , Machado-Rugolo Juliana , Capelozzi Vera L. , Nagai Maria A.
Published: June 2021

Abnormal long non-coding RNAs (lncRNAs) expression has been documented to have oncogene or tumor suppressor functions in the development and progression of cancer, emerging as promising independent biomarkers for molecular cancer stratification and patients’ prognosis. Examining the relationship between lncRNAs and the survival rates in malignancies creates new scenarios for precision medicine and targeted therapy. Breast cancer (BRCA) is a heterogeneous malignancy. Despite advances in its molecular classification, there are still gaps to explain in its multifaceted presentations and a substantial lack of biomarkers that can better predict patients’ prognosis in response to different therapeutic strategies. Here, we performed a re-analysis of gene expression data generated using cDNA microarrays in a previous study of our group, aiming to identify differentially expressed lncRNAs (DELncRNAs) with a potential predictive value for response to treatment with taxanes in breast cancer patients. Results revealed 157 DELncRNAs (90 up- and 67 down-regulated). We validated these new biomarkers as having prognostic and predictive value for breast cancer using in silico analysis in public databases. Data from TCGA showed that compared to normal tissue, MIAT was up-regulated, while KCNQ1OT1, LOC100270804, and FLJ10038 were down-regulated in breast tumor tissues. KCNQ1OT1, LOC100270804, and FLJ10038 median levels were found to be significantly higher in the luminal subtype. The ROC plotter platform results showed that reduced expression of these three DElncRNAs was associated with breast cancer patients who did not respond to taxane treatment. Kaplan–Meier survival analysis revealed that a lower expression of the selected lncRNAs was significantly associated with worse relapse-free survival (RFS) in breast cancer patients. Further validation of the expression of these DELncRNAs might be helpful to better tailor breast cancer prognosis and treatment.
Toward a Coronavirus Knowledge Graph
Authors: Zhang Peng , Bu Yi , Jiang Peng , Shi Xiaowen , Lun Bing , Chen Chongyan , Syafiandini Arida Ferti , Ding Ying , Song Min
Published: June 2021

This study builds a coronavirus knowledge graph (KG) by merging two information sources. The first source is Analytical Graph (AG), which integrates more than 20 different public datasets related to drug discovery. The second source is CORD-19, a collection of published scientific articles related to COVID-19. We combined both chemo genomic entities in AG with entities extracted from CORD-19 to expand knowledge in the COVID-19 domain. Before populating KG with those entities, we perform entity disambiguation on CORD-19 collections using Wikidata. Our newly built KG contains at least 21,700 genes, 2500 diseases, 94,000 phenotypes, and other biological entities (e.g., compound, species, and cell lines). We define 27 relationship types and use them to label each edge in our KG. This research presents two cases to evaluate the KG’s usability: analyzing a subgraph (ego-centered network) from the angiotensin-converting enzyme (ACE) and revealing paths between biological entities (hydroxychloroquine and IL-6 receptor; chloroquine and STAT1). The ego-centered network captured information related to COVID-19. We also found significant COVID-19-related information in top-ranked paths with a depth of three based on our path evaluation.
Variable Phenotypes of Epilepsy, Intellectual Disability, and Schizophrenia Caused by 12p13.33–p13.32 Terminal Microdeletion in a Korean Family: A Case Report and Literature Review
Authors: Han Ji Yoon , Park Joonhong
Published: June 2021

A simultaneous analysis of nucleotide changes and copy number variations (CNVs) based on exome sequencing data was demonstrated as a potential new first-tier diagnosis strategy for rare neuropsychiatric disorders. In this report, using depth-of-coverage analysis from exome sequencing data, we described variable phenotypes of epilepsy, intellectual disability (ID), and schizophrenia caused by 12p13.33–p13.32 terminal microdeletion in a Korean family. We hypothesized that CACNA1C and KDM5A genes of the six candidate genes located in this region were the best candidates for explaining epilepsy, ID, and schizophrenia and may be responsible for clinical features reported in cases with monosomy of the 12p13.33 subtelomeric region. On the background of microdeletion syndrome, which was described in clinical cases with mild, moderate, and severe neurodevelopmental manifestations as well as impairments, the clinician may determine whether the patient will end up with a more severe or milder end-phenotype, which in turn determines disease prognosis. In our case, the 12p13.33–p13.32 terminal microdeletion may explain the variable expressivity in the same family. However, further comprehensive studies with larger cohorts focusing on careful phenotyping across the lifespan are required to clearly elucidate the possible contribution of genetic modifiers and the environmental influence on the expressivity of 12p13.33 microdeletion and associated characteristics.
Illegal, Unreported, and Unregulated Fisheries Threatening Shark Conservation in African Waters Revealed from High Levels of Shark Mislabelling in Ghana
Authors: Agyeman Narkie Akua , Blanco-Fernandez Carmen , Steinhaussen Sophie Leonie , Garcia-Vazquez Eva , Machado-Schiaffino Gonzalo
Published: June 2021

Mislabelling of fish and fish products has attracted much attention over the last decades, following public awareness of the practice of substituting high-value with low-value fish in markets, restaurants, and processed seafood. In some cases, mislabelling includes illegal, unreported, and unregulated (IUU) fishing, contributing to overexploit substitute species that are undetectable when sold under wrong names. This is the first study of DNA barcoding to assess the level of mislabelling in fish marketed in Ghana, focusing on endangered shark species. Genetic identification was obtained from 650 base pair sequences within the cytochrome c oxidase I (COI) gene. All except one of 17 shark fillets analysed were wrongly labelled as compared with none of 28 samples of small commercial pelagic fish and 14 commercial shark samples purchased in Europe. Several substitute shark species in Ghana are endangered (Carcharhinus signatus and Isurus oxyrinchus) and critically endangered (Squatina aculeata). Shark products commercialized in Europe (n = 14) did not reveal mislabelling, thus specific shark mislabelling cannot be generalized. Although based on a limited number of samples and fish markets, the results that reveal trade of endangered sharks in Ghana markets encourage Ghanaian authorities to improve controls to enforce conservation measures.
Sedimentology and Stratigraphy of an Upper Permian Sedimentary Succession: Northern Sydney Basin, Southeastern Australia
Authors: Melehan Sean , Botziolis Chrysanthos , Maravelis Angelos G. , Catuneanu Octavian , Ruming Kevin , Holmes Erin , Collins William J.
Published: June 2021

This study integrates sedimentological and stratigraphic insights into the Upper Permian sedimentary rocks of the Wittingham, Tomago and Newcastle Coal Measures in the Northern Sydney Basin, Australia. Facies analysis documented fifteen facies that belong to seven facies associations. These facies associations correspond to different depositional environments and sub-environments including prodelta, delta-front, upper, lower delta-plain and fluvial. The stratigraphic development points to a shallowing upward trend and is reflected with fluvial deposits sitting on top of the deltaic deposits. The fluvio-deltaic contact is represented by an unconformity and displays an upward increase in sediment caliber. The delta front is mainly controlled by wave, storms- and/or river currents, even though the contribution of tides also occurs in the form of sedimentary structures that suggest tidal influence. In contrast, prodelta and delta-plain are significantly modulated by tidal currents. The impact of tides in the delta plain is fading away upward and therefore, the upper delta plain is much less impacted compared to the lower delta plain. The low abundance of wave ripples suggests that the wave action was not very important in the delta plain. Steep topographic gradients and increased sediment input are suggested, based on the limited or absent evidence of tides in the fluvial realm, related to the growing New England Orogen. In sequence stratigraphic terms, the deltaic system accumulated during highstand normal regression, while the deposition of the overlying fluvial system occurred during lowstand normal regression. The two systems are separated by a subaerial unconformity developed during an intervening forced regression. Short periods of transgression are inferred from the presence of higher frequency cycles in the delta-front.
Community Pharmacists’ Practice, Awareness, and Beliefs about Drug Disposal in Saudi Arabia
Authors: Alghadeer Sultan , Al-Arifi Mohammed N.
Published: June 2021

The awareness among Saudi people regarding the good and safe practice of drug disposal is fairly low. Community pharmacists’ potential toward drugs disposal directions and practice are not emphasized enough. Therefore, a cross sectional study was conducted in Riyadh, Saudi Arabia, to evaluate the practice, awareness and beliefs of community pharmacists about disposal of unused drugs. Out of 360 subjects who participated in the study, more than 70% returned the unused drugs to the pharmaceutical distributors. Around 80% of the participants confirmed the risk of environmental damage due to the inappropriate disposal of drugs, and 87.5% of them held themselves responsible for preventing such risk. Approximately 85% of surveyed pharmacists believed community pharmacies to be an appropriate location for the collection of unused drugs. There was no significant association between the community pharmacists’ age group and years of practice as community pharmacists with either the awareness of unused medication disposal on environmental hazards, or the beliefs about the appropriate location for collecting unused drugs (p > 0.05). The awareness and proactive accountable responsibility, along with community pharmacists’ belief of appointing pharmacies to collect unused drugs, strongly support the institution of drug take-back programs.
Impact of Job Engagement on the Quality of Nursing Services: The Effect of Person-Centered Nursing in South Korean Nurses
Authors: Kim Hyesun , Seo Kawoun
Published: June 2021

Nurses’ job engagement could help improve the quality of nursing services, and person-centered nursing is expected to play an important role in this relationship. However, little is known about the role of person-centered nursing in the association between job engagement and quality of nursing services. This study examines the moderating and mediating effects of person-centered nursing on the relationship between the job engagement and the quality of nursing services in Korean nurses. In October 2020, 200 hospital nurses were surveyed at three university hospitals. The moderating and mediating effects of person-centered nursing were determined using hierarchical regression analysis. There was a significant positive correlation between job engagement, person-centered nursing, and quality of nursing services. Person-centered nursing was found to have a mediating and moderating role in the relationship between job engagement and quality of nursing service. In conclusion, in the impact of job engagement on the quality of nursing service, it plays a buffering role, and the job engagement of nurses improves the quality of nursing services through improvement of person-centered nursing. Therefore, this study recommends the development and implementation of an educational program to foster person-centered nursing in order to improve the quality of nursing services.
Socioeconomic Inequities in Youth Participation in Physical Activity and Sports
Authors: Tandon Pooja S. , Kroshus Emily , Olsen Katharine , Garrett Kimberly , Qu Pingping , McCleery Julie
Published: June 2021

Access to opportunities for physical activity and sports, and therefore potential benefits of participation, are distributed inequitably. The aims of this study were to describe and compare youth experiences related to sport and physical activity by socioeconomic factors. A cross-sectional survey was conducted of students in 5–12th grades in King County, Washington, USA. Students were asked about physical activity and sports experiences and about demographic factors including family affluence, which was categorized as low, medium, and high. Participants were 1038 youth (50% girls, 58% non-White, and 32% from homes where languages other than English are spoken). Children from low-affluence families reported fewer days/week of physical activity, fewer sports sampled, and lower rates of ever playing sports. Greater proportions of children from low-affluence families reported these barriers to sports: (1) don’t want to get hurt, (2) don’t feel welcome on teams, (3) too expensive, and (4) transportation. Middle school children from high-affluence families had three times higher odds of meeting physical activity recommendations, and high-affluence high schoolers had three times higher odds of ever participating in sports compared to peers from low-affluence families. Socioeconomic status was inversely associated with outcomes related to youth physical activity and sports participation. The disproportionately reported barriers to sports participation are modifiable, and cross-sector solutions can help promote play equity.
Development of a Pandemic Residual Risk Assessment Tool for Building Organizational Resilience within Polish Enterprises
Authors: Ewertowski Tomasz , Butlewski Marcin
Published: June 2021

The purpose of the research paper was to develop a universal residual risk assessment tool based on the use of risk control measures related to Covid-19 in order to determine the state of organizational resilience of individual industries or organizations. The article proposes and analyzes a pandemic residual risk assessment tool, which is a simple and universal source for residual risk estimation based on a five-step consequence/probability matrix, a five-step hierarchy of risk controls, and a general formula for calculating residual risk. The methodology of the survey is based on a questionnaire with 16 questions used for the initial validation of the residual risk scale, of which six related to the potential of organizational resilience. The pilot survey was conducted in 66 enterprises in Poland. On the basis of the survey, four measures related to the use of control measures against threats after the outbreak of the Covid-19 pandemic have been proposed. These are personal protective equipment (PPE) controls, administrative controls, engineering controls, and substitution controls. Using the survey results, we estimated averages of the response results, and, on their basis, we estimated the residual risks for individual types of enterprises according to the type of business and its size. Based on the calculations, a strong correlation was found between the potential of organizational resilience and the individual use of control measures. Therefore, the main finding of the survey proves that effective risk management builds organizational resilience in enterprises. The practical implications of the study allow the management staff to find out what aspects related to the use of control measures need to be paid attention to in order to minimize residual risk.
Problematic Internet Usage and Self-Esteem in Chinese Undergraduate Students: The Mediation Effects of Individual Affect and Relationship Satisfaction
Authors: Zeng Guang , Zhang Lijin , Fung Sai-fu , Li Jingwen , Liu Yi-Man , Xiong Zi-Ke , Jiang Zhi-Quan , Zhu Fang-Fang , Chen Zhen-Ting , Luo Si-Ding , Yu Ping , Huang Qian
Published: June 2021

The aim of this cross-sectional study was to examine the mediating effects of individual affect and relationship satisfaction on the relationship between self-esteem and Problematic Internet Use (PIU). Affect was measured using the Positive and Negative Affect Schedule (PANAS), relationship satisfaction was assessed using a positive and negative semantic dimension scale, self-esteem was measured using the Rosenberg Self-Esteem Scale, and PIU was measured using the Problematic Internet Use scale with a sample of 507 Chinese university students (Mage = 20.41 years, SD = 2.49). The relationships between the variables were tested using structural equation modelling with a multiple mediation model. The results revealed that negative affect and the negative semantic dimensions of relationship satisfaction mediated the relationship between self-esteem and PIU. The implications of the results and the study’s theoretical contributions are discussed.
Effects of Mindfulness-Based Cognitive Therapy for Chronic Pain: A Multicenter Study
Authors: Pardos-Gascón Estela María , Narambuena Lucas , Leal-Costa César , Ramos-Morcillo Antonio Jesús , Ruzafa-Martínez María , van-der Hofstadt Román Carlos J.
Published: June 2021

The prevalence of chronic pain in Spain is 15%. The objective of this study was to evaluate the efficacy of mindfulness-based cognitive therapy on patients with chronic pain. A quasi-experimental design of repeated measures pre- and post-test (N = 57) was carried out at three hospitals from the province of Alicante. Self-reported assessment measurements of pain intensity, anxiety-depression symptoms, perception of health status, interference of pain on sleep, self-efficacy in pain, acceptance, and mindfulness attitude were included. The T-test indicates significant differences in intensity of present pain, mental quality of life, and depression (medium effect sizes), as well as in self-efficacy: total score, symptom management and pain control (medium effect sizes), sleep disturbances and quantity of sleep (large effect sizes). MBCT is effective in reducing many symptoms in patients with chronic pain, although its maintenance needs to be further investigated.
The Impact of Introducing Successive Biosimilars on Changes in Prices of Adalimumab, Infliximab, and Trastuzumab—Polish Experiences
Authors: Barszczewska Olga , Piechota Anna
Published: June 2021

Biosimilars are cheaper than original drugs and are thus of interest to the public. The aim of this article is to assess the benefits of introducing more than one biosimilar for the same substance (active pharmaceutical ingredient, API). The hypothesis is that the introduction of successive biosimilars of a specific original drug reduces the price of the selected API. The study focuses on drug prices varying with the successive arrival of new biosimilars. Three drugs that have at least three reimbursed biosimilars on the market were selected, two from the same therapeutic group (adalimumab and infliximab) and one (trastuzumab) representing another class of drugs. The following data were analyzed: price variation after the introduction of the first, second, and third biosimilar, and the average price reduction for all three biosimilars. Additionally, a literature review was conducted. The reimbursement of each new biosimilar is beneficial since it is associated with a price reduction in percentage terms. However, the first biosimilar brought about the greatest savings due to the higher initial prices of the original drugs and to Polish reimbursement rules. This article is helpful for when taking healthcare decisions regarding the pricing of and reimbursement for new biosimilars.
Effects of Short-Term Golden Root Extract (Rhodiola rosea) Supplementation on Resistance Exercise Performance
Authors: Williams Tyler D. , Langley Haley N. , Roberson Caleb C. , Rogers Rebecca R. , Ballmann Christopher G.
Published: June 2021

The purpose of this study was to examine the effects of short-term Golden Root Extract (GRE; Rhodiola rosea) supplementation on blood lactate, catecholamines, and performance during repeated bench press exercise. Resistance-trained males (n = 10) participated in this study. In a double-blinded, crossover, counterbalanced study design, participants supplemented with either 1500 mg/day of GRE or placebo (PL; gluten-free cornstarch) for 3 days prior to experimentation. An additional 500 mg dose was ingested 30 min prior to exercise testing. During each exercise trial, participants completed 2 repetitions of bench press at 75% of one-repetition maximum (1RM) as explosively as possible. A linear position transducer was used to measure mean concentric velocity. After 5 min of rest, participants completed 3 sets × repetitions to failure (RTF) at 75% 1RM separated by 2 min of rest between each set. A capillary blood sample was obtained pre- (PRE) and immediately post- (POST) exercise to measure blood concentrations lactate (LA), epinephrine (EPI), and norepinephrine (NE). Mean concentric velocity was significantly higher with GRE when compared to PL (p = 0.046). However, total RTF were significantly lower with GRE versus PL (p < 0.001). Regardless of treatment, LA was significantly higher Post versus Pre (p < 0.001), but GRE resulted in greater Post values compared to PL (p = 0.049). EPI and NE increased in both conditions Pre to Post (p < 0.001). However, Pre NE was significantly higher with GRE versus PL (p = 0.008). Findings indicate that short-term GRE supplementation increases mean bench press velocity but decreases bench press repetition volume. Furthermore, GRE resulted in higher NE levels and blood lactate following exercise. Thus, supplementing with GRE may enhance explosive resistance training performance but may also impair upper body strength-endurance.
Toilet Paper, Minced Meat and Diabetes Medicines: Australian Panic Buying Induced by COVID-19
Authors: Engstrom Teyl , Baliunas Dolly O. , Sly Benjamin P. , Russell Anthony W. , Donovan Peter J. , Krausse Heike K. , Sullivan Clair M. , Pole Jason D.
Published: June 2021

The COVID-19 pandemic has impacted the management of non-communicable diseases in health systems around the world. This study aimed to understand the impact of COVID-19 on diabetes medicines dispensed in Australia. Publicly available data from Australia’s government subsidised medicines program (Pharmaceutical Benefits Scheme), detailing prescriptions by month dispensed to patients, drug item code and patient category, was obtained from January 2016 to November 2020. This study focused on medicines used in diabetes care (Anatomical Therapeutical Chemical code level 2 = A10). Number of prescriptions dispensed were plotted by month at a total level, by insulins and non-insulins, and by patient category (general, concessional). Total number of prescriptions dispensed between January and November of each year were compared. A peak in prescriptions dispensed in March 2020 was identified, an increase of 35% on March 2019, compared to average growth of 7.2% in previous years. Prescriptions dispensed subsequently fell in April and May 2020 to levels below the corresponding months in 2019. These trends were observed across insulins, non-insulins, general and concessional patient categories. The peak and subsequent dip in demand have resulted in a small unexpected overall increase for the period January to November 2020, compared to declining growth for the same months in prior years. The observed change in consumer behaviour prompted by COVID-19 and the resulting public health measures is important to understand in order to improve management of medicines supply during potential future waves of COVID-19 and other pandemics.
COVID-19 Pandemic Causing Depression in Different Sociodemographic Groups in Saudi Arabia
Authors: Sonbol Hana , Alahdal Hadil M. , Alanazi Rasis A. , Alsamhary Khawla , Ameen Fuad
Published: June 2021

COVID-19 disease was announced as a global pandemic in March 2020 by the World health organization (WHO). Saudi Arabia was among the first countries to enforce restriction measures such as closing schools, remote working, and a travel ban. We aim to evaluate the impact of the COVID-19 pandemic on people’s depression in Saudi Arabia. A cross-sectional online survey of 1109 participants was conducted during the curfew between 18th of May and 11th of June 2020. An online questionnaire included questions about the commitment to follow the precautionary measures, knowledge on COVID-19, and depression. Depression was assessed with the Impact of Event Scale-Revised method. Females, unmarried individuals, elderly persons, parents of young children, unemployed, and small families were more likely to be depressed. Education level did not explain the differences in depression. However, the more knowledge the participants had about COVID-19 the better they followed the restrictions. A regression analysis revealed that the commitment of a person to follow the restrictions increased his/her depression symptoms. Attention should be paid to different groups of people in future psychiatric planning.
New Onset of Hepatic Steatosis Post-Severe Multisystem Inflammatory Syndrome in Children (MIS-C): A Case Report
Authors: Sica Rossella , Pennoni Serena , Penta Laura , Di Cara Giuseppe , Verrotti Alberto
Published: June 2021

The emergence of Multisystem Inflammatory Syndrome (MIS-C) following SARS-CoV-2 infection in children and adolescents provided a new diagnostic and management challenge as there is limited knowledge about this condition and its natural history. In existing literature on MIS-C, there are currently no data about long-term outcomes. We report the case of a 14-year-old boy, with no significant past medical history, who presented a condition of multiorgan dysfunction due to MIS-C, after a SARS CoV-2 infection, and subsequent clinical-laboratory signs of hepatic steatosis at short-term follow-up. The case suggests how hepatic steatosis may be a possible sequela following SARS-CoV-2 infection, MIS-C and its medical treatment. Therefore, a close and long-term follow-up is needed to establish the pathophysiology and the evolution of this condition in patients following MIS-C.
The Effects of Active Video Games on Health-Related Physical Fitness and Motor Competence in Children and Adolescents with Healthy Weight: A Systematic Review and Meta-Analysis
Authors: Comeras-Chueca Cristina , Marin-Puyalto Jorge , Matute-Llorente Angel , Vicente-Rodriguez German , Casajus Jose A. , Gonzalez-Aguero Alex
Published: June 2021

(1) Background: Poor levels of physical fitness and motor skills are problems for today’s children. Active video games (AVG) could be an attractive strategy to help address them. The aim was to investigate the effects of AVG on health-related physical fitness and motor competence in children and adolescents with healthy weight. (2) Methods: Randomized and non-randomized controlled trials investigating the effects of AVG programs on health-related physical fitness and motor competence were included. Two different quality assessment tools were used to measure the risk of bias. Twenty articles met the inclusion criteria and the variables of interest were body mass index (BMI), body fat, cardiorespiratory fitness (CRF), muscular fitness and motor competence. (3) Results: AVG interventions seem to have benefits in BMI when lasting longer than 18 weeks (SMD, −0.590; 95% IC, −1.071, −0.108) and in CRF (SMD, 0.438; 95% IC, 0.022, 0.855). AVG seems to be a promising tool to improve muscular fitness and motor competence but the effects are still unclear due to the lack of evidence. (4) Conclusions: AVG seem to be an effective tool for improving some components of health-related physical fitness and motor competence in healthy-weight children and adolescents, but the effect on some fitness components needs further research. Therefore, AVG may be included as a strategy to improve health.
Factors Influencing Adjustment to Remote Work: Employees’ Initial Responses to the COVID-19 Pandemic
Authors: van Zoonen Ward , Sivunen Anu , Blomqvist Kirsimarja , Olsson Thomas , Ropponen Annina , Henttonen Kaisa , Vartiainen Matti
Published: June 2021

The COVID-19 crisis has disrupted when, where, and how employees work. Drawing on a sample of 5452 Finnish employees, this study explores the factors associated with employees’ abrupt adjustment to remote work. Specifically, this study examines structural factors (i.e., work independence and the clarity of job criteria), relational factors (i.e., interpersonal trust and social isolation), contextual factors of work (i.e., change in work location and perceived disruption), and communication dynamics (i.e., organizational communication quality and communication technology use (CTU)) as mechanisms underlying adjustment to remote work. The findings demonstrate that structural and contextual factors are important predictors of adjustment and that these relationships are moderated by communication quality and CTU. Contrary to previous research, trust in peers and supervisors does not support adjustment to remote work. We discuss the implications of these findings for practice during and beyond times of crisis.
The Usage of Mobile Apps to Fight Violence against Women: A Survey on a Sample of Female Students Belonging to an Italian University
Authors: Tozzo Pamela , Gabbin Andrea , Politi Caterina , Frigo Anna Chiara , Caenazzo Luciana
Published: June 2021

The prevalence of violence against women continues to grow and this plague has had a huge impact from a clinical, social and judicial point of view. For this reason, alongside the efforts made at the legislative level to prevent the phenomenon and to improve assistance to victims in recent years, efforts to contain and better manage this phenomenon have also grown in the extra-legislative sphere: for example, through the application of new technological solutions and safety planning. In recent years, there has been an increase in the marketing of mobile phone apps dedicated to the prevention of violence against women, with different functions and different objectives. The purpose of this study is to investigate the knowledge and propensity to download this type of app in a group of 1782 Italian female university students. This research was performed using an online questionnaire administered to female students attending four different courses (law, medicine, healthcare professionals and political sciences) at one Italian university. Chi-square or Fisher’s exact test was used to analyze associations between responses to questionnaire and the type and the year of course. The results show that 62.6% of our sample are unaware of the existence of these apps and that 79.5% of the sample would be willing to download one in the future. With regard to whom to turn to after a violent incident, the majority of those interviewed (43.9%) would turn to the police and not to health facilities. According to our findings, law female students (52.7%) think, more than any other category, that the most effective way to improve public safety and reduce the number of victims lies in legislative solutions. Our results suggest that, although this type of technology may be promising, it is necessary to improve the knowledge and dissemination of these apps in order to make them a useful tool for prevention, education and assistance in cases of violence against women.
The Association Pattern between Ambient Temperature Change and Leukocyte Counts
Authors: Hung Shih-Chiang , Yang Chen-Cheng , Liu Chu-Feng , Kung Chia-Te , Lee Wen-Huei , Ho Chi-Kung , Chuang Hung-Yi , Yu Hsin-Su
Published: June 2021

Ambient temperature change is one of the risk factors of human health. Moreover, links between white blood cell counts (WBC) and diseases have been revealed in the literature. Still, we do not know of any association between ambient temperature change and WBC counts. The aim of our study is to investigate the relationship between ambient temperature change and WBC counts. We conducted this two-year population-based observational study in Kaohsiung city, recruiting voluntary community participants. Total WBC and differential counts, demographic data and health hazard habits were collected and matched with the meteorological data of air-quality monitoring stations with participants’ study dates and addresses. Generalized additive models (GAM) with penalized smoothing spline functions were performed for the trend of temperature changes and WBC counts. There were 9278 participants (45.3% male, aged 54.3 ± 5.9 years-old) included in analysis. Compared with stable weather conditions, the WBC counts were statistically higher when the one-day lag temperature changed over 2 degrees Celsius, regardless of whether colder or hotter. We found a V-shaped pattern association between WBC counts and temperature changes in GAM. The ambient temperature change was associated with WBC counts, and might imply an impact on systematic inflammation response.
Linking Economic Growth, Urbanization, and Environmental Degradation in China: What Is the Role of Hydroelectricity Consumption?
Authors: Adebayo Tomiwa Sunday , Agboola Mary Oluwatoyin , Rjoub Husam , Adeshola Ibrahim , Agyekum Ephraim Bonah , Kumar Nallapaneni Manoj
Published: June 2021

Achieving environmental sustainability has become a global initiative whilst addressing climate change and its effects. Thus, this research re-assessed the EKC hypothesis in China and considered the effect of hydroelectricity use and urbanization, utilizing data from 1985 to 2019. The autoregressive distributed lag (ARDL) bounds testing method was utilized to assess long-run cointegration, which is reinforced by a structural break. The outcome of the ARDL bounds test confirmed cointegration among the series. Furthermore, the ARDL revealed that both economic growth and urbanization trigger environmental degradation while hydroelectricity improves the quality of the environment. The outcome of the ARDL also validated the EKC hypothesis for China. In addition, the study employed the novel gradual shift causality test to capture causal linkage among the series. The advantage of the gradual shift causality test is that it can capture gradual or smooth shifts and does not necessitate previous information of the number, form of structural break(s), or dates. The outcomes of the causality test revealed causal connections among the series of interest.
Are There Any Differences in Abdominal Activation between Women and Men during Hypopressive Exercises?
Authors: Da Cuña-Carrera Iria , Alonso-Calvete Alejandra , Lantarón-Caeiro Eva M. , Soto-González Mercedes
Published: June 2021

This study analyzes the effects of hypopressive exercises on the abdominal thickness of healthy subjects and compares the performance between women and men. We conducted a transversal observational study in 98 subjects (63% women). The muscle thickness is analyzed in transversus abdominis, internal oblique, external oblique, and rectus abdominis with ultrasound imaging at rest and during the hypopressive exercise (HE) in supine and standing position. Comparisons between rest and hypopressive exercise are carried out in the two different positions and between women and men. In the supine position, there is a significant activation of the transversus abdominis and internal oblique during hypopressive exercise (p < 0.001), and it is similar in both sexes, the external oblique is only activated significantly by men (p < 0.001) and rectus abdominis had no significant activation (p > 0.05). Our results show that standing transversus abdominis and external oblique significantly increased their thickness during HE with higher effects in men. Internal oblique also increased significantly, but with higher effects in women, and rectus abdominis had no significant increase. Men had similar effects to women during HE, with an activation of the deepest abdominal muscles. The unequal anatomy and the position could explain the different results obtained between the sexes.
Hydrological Modeling of Green Infrastructure to Quantify Its Effect on Flood Mitigation and Water Availability in the High School Watershed in Tucson, AZ
Authors: Korgaonkar Yoganand , Guertin David Phillip , Meixner Thomas , Goodrich David C
Published: June 2021

Green Infrastructure (GI) practices are being implemented in numerous cities to tackle stormwater management issues and achieve co-benefits such as mitigating heat island effects and air pollution, as well as water augmentation, health, and economic benefits. Tucson, Arizona is a fast-growing city in the semiarid region of the southwest United States and provides a unique landscape in terms of urban hydrology and stormwater management, where stormwater is routed along the streets to the nearest ephemeral washes. Local organizations have implemented various GI practices, such as curb cuts, traffic chicanes, roof runoff harvesting, and retention basins, to capture the excess runoff and utilize it on-site. This study models the 3.31 km2 High School watershed in central Tucson using the Automated Geospatial Watershed Assessment (AGWA) tool and the Kinematic Runoff and Erosion (KINEROS2) model. Each parcel in the watershed was individually represented using the KINEROS2 Urban element to simulate small-scale flow-on/flow-off processes. Seven different configurations of GI implementation were simulated using design storms, and we stochastically generated 20 years of precipitation data to understand the effects of GI implementation on flood mitigation and long-term water availability, respectively. The design storm analysis indicates that the configuration designed to mimic the current level of GI implementation, which includes 175 on-street basins and 37 roof runoff harvesting cisterns, has minimum (<2%) influence on runoff volume. Furthermore, the analysis showed that the current level of GI implementation caused an increase (<1%) in peak flows at the watershed outlet but predicted reduced on-street accumulated volumes (>25%) and increased water availability via GI capture and infiltration. When the GI implementation was increased by a factor of two and five, a larger reduction of peak flow (<8% and <22%, respectively) and volume (<3% and <8%, respectively) was simulated at the watershed outlet. The 20-year analysis showed that parcels with roof runoff harvesting cisterns were able to meet their landscape irrigation demands throughout the year, except for the dry months of May and June. Additionally, stormwater captured and infiltrated by the on-street basins could support xeric vegetation for most of the year, except June, where the water demand exceeded volume of water infiltrated in the basins. The current level of GI implementation in the High School watershed may not have significant large-scale impacts, but it provides numerous benefits at the parcel, street, and small neighborhood scales.
Classification of Airborne Laser Scanning Point Cloud Using Point-Based Convolutional Neural Network
Authors: Zhu Jianfeng , Sui Lichun , Zang Yufu , Zheng He , Jiang Wei , Zhong Mianqing , Ma Fei
Published: June 2021

In various applications of airborne laser scanning (ALS), the classification of the point cloud is a basic and key step. It requires assigning category labels to each point, such as ground, building or vegetation. Convolutional neural networks have achieved great success in image classification and semantic segmentation, but they cannot be directly applied to point cloud classification because of the disordered and unstructured characteristics of point clouds. In this paper, we design a novel convolution operator to extract local features directly from unstructured points. Based on this convolution operator, we define the convolution layer, construct a convolution neural network to learn multi-level features from the point cloud, and obtain the category label of each point in an end-to-end manner. The proposed method is evaluated on two ALS datasets: the International Society for Photogrammetry and Remote Sensing (ISPRS) Vaihingen 3D Labeling benchmark and the 2019 IEEE Geoscience and Remote Sensing Society (GRSS) Data Fusion Contest (DFC) 3D dataset. The results show that our method achieves state-of-the-art performance for ALS point cloud classification, especially for the larger dataset DFC: we get an overall accuracy of 97.74% and a mean intersection over union (mIoU) of 0.9202, ranking in first place on the contest website.
Gastrin and the Moderate Hypergastrinemias
Authors: Rehfeld Jens F.
Published: June 2021

The antral hormone gastrin potently regulates gastric acid secretion and fundic mucosal growth. Consequently, appropriate gastrin secretion and plasma concentrations are important for the early phases of digestion. This review describes as the first premise the normal biogenesis of gastrin in the antral mucosa, but also mentions the extraantral expression. Subsequently, the molecular nature and concentration levels of gastrin in serum or plasma are overviewed. Third, assays for accurate measurements of plasma or serum concentrations are commented. Finally, the problem of moderate hypergastrinemia due to Helicobacter pylori infections and/or treatment with proton-pump inhibitors (PPI) is discussed. The review concludes that accurate measurement of the true concentrations of bioactive gastrins in plasma is important. Moreover, it suggests that moderate hypergastrinemias are also essential health issues that require serious attention.
CARD9 Deficiency Increases Hippocampal Injury Following Acute Neurotropic Picornavirus Infection but Does Not Affect Pathogen Elimination
Authors: Pavasutthipaisit Suvarin , Stoff Melanie , Ebbecke Tim , Ciurkiewicz Malgorzata , Mayer-Lambertz Sabine , Störk Theresa , Pavelko Kevin D. , Lepenies Bernd , Beineke Andreas
Published: June 2021

Neurotropic viruses target the brain and contribute to neurologic diseases. Caspase recruitment domain containing family member 9 (CARD9) controls protective immunity in a variety of infectious disorders. To investigate the effect of CARD9 in neurotropic virus infection, CARD9−/− and corresponding C57BL/6 wild-type control mice were infected with Theiler’s murine encephalomyelitis virus (TMEV). Brain tissue was analyzed by histology, immunohistochemistry and molecular analyses, and spleens by flow cytometry. To determine the impact of CARD9 deficiency on T cell responses in vitro, antigen presentation assays were utilized. Genetic ablation of CARD9 enhanced early pro-inflammatory cytokine responses and accelerated infiltration of T and B cells in the brain, together with a transient increase in TMEV-infected cells in the hippocampus. CARD9−/− mice showed an increased loss of neuronal nuclear protein+ mature neurons and doublecortin+ neuronal precursor cells and an increase in β-amyloid precursor protein+ damaged axons in the hippocampus. No effect of CARD9 deficiency was found on the initiation of CD8+ T cell responses by flow cytometry and co-culture experiments using virus-exposed dendritic cells or microglia-enriched glial cell mixtures, respectively. The present study indicates that CARD9 is dispensable for the initiation of early antiviral responses and TMEV elimination but may contribute to the modulation of neuroinflammation, thereby reducing hippocampal injury following neurotropic virus infection.
NLRP3 and Infections: β-Amyloid in Inflammasome beyond Neurodegeneration
Authors: Sita Giulia , Graziosi Agnese , Hrelia Patrizia , Morroni Fabiana
Published: June 2021

Amyloid beta (Aβ)-induced abnormal neuroinflammation is recognized as a major pathological feature of Alzheimer’s disease (AD), which results in memory impairment. Research exploring low-grade systemic inflammation and its impact on the development and progression of neurodegenerative disease has increased. A particular research focus has been whether systemic inflammation arises only as a secondary effect of disease, or it is also a cause of pathology. The inflammasomes, and more specifically the NLRP3 inflammasome, are crucial components of the innate immune system and are usually activated in response to infection or tissue damage. Although inflammasome activation plays critical roles against various pathogens in host defense, overactivation of inflammasome contributes to the pathogenesis of inflammatory diseases, including acute central nervous system (CNS) injuries and chronic neurodegenerative diseases, such as AD. This review summarizes the current literature on the role of the NLRP3 inflammasome in the pathogenesis of AD, and its involvement in infections, particularly SARS-CoV-2. NLRP3 might represent the crossroad between the hypothesized neurodegeneration and the primary COVID-19 infection.
Selective Inhibition of Heparan Sulphate and Not Chondroitin Sulphate Biosynthesis by a Small, Soluble Competitive Inhibitor
Authors: Maciej-Hulme Marissa L. , Dubaissi Eamon , Shao Chun , Zaia Joseph , Amaya Enrique , Flitsch Sabine L. , Merry Catherine L. R.
Published: June 2021

The glycosaminoglycan, heparan sulphate (HS), orchestrates many developmental processes. Yet its biological role has not yet fully been elucidated. Small molecule chemical inhibitors can be used to perturb HS function and these compounds provide cheap alternatives to genetic manipulation methods. However, existing chemical inhibition methods for HS also interfere with chondroitin sulphate (CS), complicating data interpretation of HS function. Herein, a simple method for the selective inhibition of HS biosynthesis is described. Using endogenous metabolic sugar pathways, Ac4GalNAz produces UDP-GlcNAz, which can target HS synthesis. Cell treatment with Ac4GalNAz resulted in defective chain elongation of the polymer and decreased HS expression. Conversely, no adverse effect on CS production was observed. The inhibition was transient and dose-dependent, affording rescue of HS expression after removal of the unnatural azido sugar. The utility of inhibition is demonstrated in cell culture and in whole organisms, demonstrating that this small molecule can be used as a tool for HS inhibition in biological systems.