bluuebunny/crossref_metadata_2025 huggingface dataset:
New cationic polyprenyl derivative proposed as a lipofecting agent.
Authors: Madeja Zbigniew , Rak Monika , Wybieralska Ewa , Rózański Igor , Masnyk Marek , Chmielewski Marek , Lysek Robert , Chojnacki Tadeusz , Jankowski Wiesław , Ciepichal Ewa , Swiezewska Ewa , Tekle Michael , Dallner Gustav
Published: November 2019

Cationic linear poly-cis-isoprenoid prepared from natural plant polyprenol in a mixture with dioleyl phosphatidylethanolamine was found to be an effective lipofection agent for eukaryotic cells. The transfecting activity is related to the poly-cis structure of the polyprenyl chain.
Evaluation of HI-6 oxime: potential use in protection of human acetylcholinesterase inhibited by antineoplastic drug irinotecan and its cyto/genotoxicity in vitro.
Authors: Radić Bozica , Vrdoljak Ana Lucić , Zeljezić Davor , Fuchs Nino , Berend Suzana , Kopjar Nevenka
Published: November 2019

The function of acetylcholinesterase (AChE) is the rapid hydrolysis of the neurotransmitter acetylcholine (ACh), which is involved in the numerous cholinergic pathways in both the central and the peripheral nervous system. Therefore, AChE measurement is of high value for therapy management, especially during the course of intoxication with different chemicals or drugs that inhibit the enzyme. Pyridinium or bispyridinium aldoximes (oximes) are able to recover the activity of the inhibited enzyme. Since their adverse effects are not well elucidated, in this study the efficiency of HI-6 oxime in protection and/or reactivation of human erythrocyte AChE inhibited by the antineoplastic drug irinotecan as well as its cyto/genotoxicity in vitro were investigated. HI-6 was effective in protection of AChE and increased its activity up to 30%; the residual activity after irinotecan inhibition was 7%. Also, it reactivated the enzyme previously inhibited by 50% irinotecan (4.6 microg/ml) applied at 1/4 of the IC50 value. The tested concentrations of HI-6 exhibited acceptable genotoxicity towards white blood cells, as estimated by the alkaline comet assay, DNA diffusion assay and cytogenetic endpoints (structural chromosome aberrations and cytokinesis-block micronucleus assay). The results obtained warrant the further investigation of HI-6 in vivo, as well as its development for possible application in chemotherapy.
Overexpression of BimSs3, the novel isoform of Bim, can trigger cell apoptosis by inducing cytochrome c release from mitochondria.
Authors: Liu Lingfeng , Chen Jinzhong , Zhang Jiayi , Ji Chaoneng , Zhang Xiaomeng , Gu Shaohua , Xie Yi , Mao Yumin
Published: November 2019

Bim is defined as the pro-apoptotic BH3-only protein of the Bcl-2 family, which is a critical sensor and mediator in the mitochondrial-dependent apoptosis. In a previous work, we have cloned a novel transcript of Bim (GenBank accession number: AY305716) from the fetal brain cDNA, which is widely expressed in some carcinoma tissues and normal human tissues. According to the sequence analysis and the newly-defined nomenclature system of Bim isoforms (Adachi et al., 2005, Cell Death Differ 2: 192), we term it BimSs3 according to its characteristic structure. The subcellular location analysis indicated that the fused protein GFP-BimSs3 is distributed in the whole cell, mainly to the nucleus. Overexpression of BimSs3 in HEK293 cells causes apoptosis (28.16 +/- 1.55%) compared to the negative control (5.44 +/- 2.63%). It also causes cytochrome c release from the mitochondrial fraction to the cytosolic fraction during apoptosis. Western blotting assay indicates the molecular mass of GFP-BimSs3 is approximately 31.0 kDa (GFP: 27 kDa). Hence the open reading frame of BimSs3 may initiate at the second ATG and encodes a 36 amino-acid peptide with BH3 domain.
The involvement of Na+/K(+)-ATPase in the development of platelet procoagulant response.
Authors: Tomasiak Marian , Stelmach Halina , Rusak Tomasz , Ciborowski Michał , Radziwon Piotr
Published: November 2019

In circulation, platelets may come into contact with both exogenous (cardiac glycoside treatment) and endogenously produced inhibitors of Na+/K(+)-ATPase. We examined whether blocking of platelet Na+/K(+)-ATPase by ouabain results in generation of procoagulant activity. It was shown that an in vitro treatment of platelets with ouabain (20-200 microM for 20 to 60 min) is associated with an intracellular accumulation of sodium ([Na+](i)), generation of a weak calcium signal, and expression of procoagulant activity. The ouabain-induced procoagulant response was dose- and time-related, less pronounced than that evoked by collagen and similar to that produced by gramicidin, not affected by EDTA or aspirin, and strongly reduced in the absence of extracellular Na+ or by hyperosmolality. Flow cytometry studies revealed that ouabain treatment results in a unimodal left shift in the forward and side scatter of the entire platelet population indicating morphological changes of the plasma membrane. The shift was dose related, weaker than that evoked by collagen and similar to that produced by gramicidin. Ouabain-treated platelets express phosphatidylserine (PS). The ouabain-evoked PS expression was dose- and time-dependent, weaker than that produced by collagen and similar to that evoked by gramicidin. Electronic cell sizing measurements showed a dose-dependent increase in mean platelet volume upon treatment with ouabain. Hypoosmotically-evoked platelet swelling resulted in the appearance of procoagulant activity. Thromboelastography measurements indicate that, in whole blood, nanomolar (50-1000 nM, 15 min) concentrations of ouabain significantly accelerate the rate of clot formation initiated by contact and high extracellular concentration of calcium. We conclude that inefficiently operating platelet Na+/K(+)-ATPase results in a rise in [Na+](i). An increase in [Na+](i) and the swelling associated with it may produce PS exposure and a rise in membrane curvature leading to the generation of a procoagulant activity.
Effect of daidzein, a soy isoflavone, on bone metabolism in Cd-treated ovariectomized rats.
Authors: Om Ae-Son , Shim Jae-Young
Published: November 2019

This study compared the ability of daidzein, a soy isoflavone, with that of 17beta-estradiol to prevent bone loss in cadmium (Cd)-exposed ovariectomized (OVX) rats during growth. Four week-old female Wistar rats were randomly assigned to five treatment groups of 9 rats each, either (1) sham-operated (SH); (2) OVX and placed on experimental diets (OVX); (3) OVX fed 50 ppm of CdCl2 (OVX-Cd); (4) OVX fed 50 ppm of CdCl2 and 10 microg of daidzein per kg of body mass (OVX-CD-D); or (5) OVX fed 50 ppm of CdCl2 and 10 microg of estrogen per kg of body mass (OVX-CD-E). All rats were given free access to AIN-76 modified diet and drinking water, with or without Cd, for 8 weeks. The OVX groups gained more (P < 0.05) body mass than the SH group. Femoral mass was increased by feeding daidzein and estradiol, whereas femoral length was not (P > 0.05) significantly different among groups. Femoral breaking force was not significantly different among groups, however, femoral BMD was significantly lower in OVX-Cd than in the SH and OVX groups. Morphologically proliferative cartilage and hypertrophic cells in femur showed normal distribution in OVX-Cd-D and OVX-Cd-E groups unlike those in OVX-Cd group. These findings suggest that Cd-OVX-induced osteopenia or osteoporosis probably results from an increase in bone turnover.
Androgen receptor and c-Myc transcription factors as putative partners in the in vivo cross-talk between androgen receptor-mediated and c-Met-mediated signalling pathways.
Authors: Dudkowska Magdalena , Jaworski Tomasz , Grzelakowska-Sztabert Barbara , Manteuffel-Cymborowska Małgorzata
Published: November 2019

Cross-talk between two signal transduction pathways leads to a negative regulation of androgen-induced ornithine decarboxylase (ODC) gene expression in the mouse kidney. One pathway is triggered by testosterone via the intracellular androgen receptor, AR, and the other is induced by antifolate CB 3717 or folate via hepatocyte growth factor and its cell membrane receptor c-Met. Here we report the studies of the expression of AR and c-Myc transcription factors involved in ODC transactivation. Administration of CB 3717 or folate decreased the expression of AR. In contrast, testosterone did not modify AR mRNA content but augmented the AR protein. Furthermore, we demonstrate that administration of folate, but not testosterone, increases c-Myc transcript and protein level. We also document that activation of both examined pathways does not decrease the testosterone-induced AR protein level, but markedly increases c-Myc protein which is nearly 2-fold up-regulated compared to its level evoked solely by testosterone. We suspect that this pronounced increase of c-Myc protein might have functional consequences mirrored by down-regulated expression of AR target genes, among them ODC.
Thiamine deficiency caused by thiamine antagonists triggers upregulation of apoptosis inducing factor gene expression and leads to caspase 3-mediated apoptosis in neuronally differentiated rat PC-12 cells.
Authors: Chornyy Sergiy , Parkhomenko Julia , Chorna Nataliya
Published: November 2019

Recent evidence suggests that alterations in oxidative metabolism induced by thiamine deficiency lead to neuronal cell death. However, the molecular mechanisms underlying this process are still under extensive investigation. Here, we report that rat pheochromocytoma PC-12 cells differentiated in the presence of NGF into neurons undergo apoptosis due to thiamine deficiency caused by antagonists of thiamine - amprolium, pyrithiamine and oxythiamine. Confocal laser scanning fluorescence microscopy revealed that annexin V binds to PC-12 cells in presence of thiamine antagonists after 72 h incubation. Results also show that thiamine antagonists trigger upregulation of gene expression of mitochondrial-derived apoptosis inducing factor, DNA fragmentation, cleavage of caspase 3 and translocation of active product to the nucleus. We therefore propose that apoptosis induced by amprolium, pyrithiamine or oxythiamine occurs via the mitochondria-dependent caspase 3-mediated signaling pathway. In addition, our data indicate that pyrithiamine and oxythiamine are more potent inducers of apoptosis than amprolium.
Gamijeonssibaekchulsan regulates mast cell-mediated anaphylactic reaction.
Authors: Moon Phil Dong , Shin Hye Young , Na Ho Jeong , Jeong Hyun Ja , Kim Su Jin , Um Jae Young , Park Rae Kil , Kim Hyung Min , Hong Seung Heon
Published: November 2019

Gamijeonssibaekchulsan (GJBS) is a typical Oriental medicine prescription which has been used in Korea for the treatment of allergic diseases and the development of physical strength. However, as yet there is no clear explanation of how GJBS affects the anaphylactic reaction and the immune function. In the present study murine models and MOLT-4 cells, a T cell line, were used to investigate these effects. Compound 48/80-induced systemic anaphylactic shock and ear swelling response were firstly analyzed. We also assayed histamine release and passive cutaneous anaphylaxis (PCA) in mice and cytokine productions in MOLT-4 cells. GJBS significantly inhibits compound 48/80-induced systemic anaphylactic shock and ear swelling response. GJBS also inhibits histamine release from rat peritoneal mast cells induced by compound 48/80. PCA activated by anti-dinitrophenyl immunoglobulin E is attenuated by GJBS. However, GJBS dose not affect the production of interferon-gamma, interleukin (IL)-2, and IL-4 in MOLT-4 cells. These results indicate that GJBS has a potential regulatory effect on allergic reactions that are mediated by mast cells.
Two-electron reduction of quinones by Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase: quantitative structure-activity relationships.
Authors: Miseviciene Lina , Anusevicius Zilvinas , Sarlauskas Jonas , Harris Richard J , Scrutton Nigel S , Cenas Narimantas
Published: November 2019

In order to clarify the poorly understood mechanisms of two-electron reduction of quinones by flavoenzymes, we examined the quinone reductase reactions of a member of a structurally distinct old yellow enzyme family, Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase (PETNR). PETNR catalyzes two-electron reduction of quinones according to a 'ping-pong' scheme. A multiparameter analysis shows that the reactivity of quinones increases with an increase in their single-electron reduction potential and pK(a) of their semiquinones (a three-step (e(-),H(+),e(-)) hydride transfer scheme), or with an increase in their hydride-transfer potential (E(7)(H(-))) (a single-step (H(-)) hydride transfer scheme), and decreases with a decrease in their van der Waals volume. However, the pH-dependence of PETNR reactivity is more consistent with a single-step hydride transfer. A comparison of X-ray data of PETNR, mammalian NAD(P)H : quinone oxidoreductase (NQO1), and Enterobacter cloacae nitroreductase, which reduce quinones in a two-electron way, and their reactivity revealed that PETNR is much less reactive, and much less sensitive to the quinone substrate steric effects than NQO1. This may be attributed to the lack of pi-pi stacking between quinone and the displaced aromatic amino acid in the active center, e.g., with Phe-178' in NQO1.
A novel alpha-glucosidase from the moss Scopelophila cataractae.
Authors: Yamasaki Yoshiki , Nakashima Susumu , Konno Haruyoshi
Published: November 2019

Scopelophila cataractae is a rare moss that grows on copper-containing soils. S. cataractae protonema was grown on basal MS medium containing copper. A starch-degrading activity was detected in homogenates of the protonema, after successive extraction with phosphate buffer and buffer containing 3 M LiCl. Buffer-soluble extract (BS) and LiCl-soluble extract (LS) readily hydrolyzed amylopectin to liberate only glucose, which shows that alpha-glucosidase (EC 3.2.1.20) in BS and LS hydrolyzed amylopectin. The K(m) value of BS for maltose was 0.427. The K(m) value of BS for malto-oligosaccharide decreased with an increase in the molecular mass of the substrate. The value for maltohexaose was 0.106, which is about four-fold lower than that for maltose. BS was divided into two fractions of alpha-glucosidase (BS-1 and BS-2) by isoelectric focusing. The isoelectric points of these two enzymes were determined to be 4.36 (BS-1) and 5.25 (BS-2) by analytical gel electrofocusing. The two enzymes readily hydrolyzed malto-oligosaccharides. The two enzymes also hydrolyzed amylose, amylopectin and soluble starch at a rate similar to that with maltose. The two enzymes readily hydrolyzed panose to liberate glucose and maltose (1 : 1), and the K(m) value of BS for panose was similar to that for maltotriose, whereas the enzymes hydrolyzed isomaltose only weakly. With regard to substrate specificity, the two enzymes in BS are novel alpha-glucosidases. The two enzymes also hydrolyzed beta-limit dextrin, which has many alpha-1,6-glucosidic linkages near the non-reducing ends, more strongly than maltose, which shows that they do not need a debranching enzyme for starch digestion. The starch-degrading activity of BS was not inhibited by p-chloromercuribenzoic acid or alpha-amylase inhibitor. When amylopectin was treated with BS and LS in phosphate buffer, pH 6.0, glucose, but not glucose-1-phosphate, was detected, showing that the extracts did not contain phosphorylase but did contain an alpha-glucosidase. These results show that alpha-glucosidases should be capable of complete starch digestion by themselves in cells of S. cataractae.
Evaluation of the Effects of Chronic Injection of D-Galactose on Auditory Brainstem Responses as a Model for Studying Age-Related Hearing Loss
Authors: Tavanai Elham , Mohammadkhani Ghassem
Published: November 2019

The D-galactose induced mimetic aging rat model has been widely used in studies of age-associated diseases recently. Evidence indicates that D-GAL could also play a key role in age-related hearing loss. However, there is conflicting data about the relationship between the D-GAL injection and tone-burst auditory brainstem responses (ABRs). The present study aimed to compare ABRs in D-GAL injected rats compared with young and naturally aged rats. Tone-burst ABR was recorded and analyzed at the frequencies of 4,6,8,12 and 16 kHz in male young (3-month-old, n=10), naturally aging (18-month-old, n=10) and D-GAL injected (3-month-old, 500 mg/kg D-GAL injection for 8 weeks, n=10) Wistar rats. When the ABRs thresholds obtained in the D-GAL group and the natural aging group were compared with the thresholds in the young group, we observed a significant increase in thresholds, which affected all of the frequencies (P<0.05). A statistically significant decrease in amplitude of wave PI at 4 and 8 kHz, PII at 4,8 kHz, PIV at 4,6,8,12 and 16 kHz was also observed in naturally aging group compared with young group. However, in D-GAL group, a significant difference was exclusively detected in amplitude of PIII at 4 kHz. Latency did not reveal any significant difference between the groups (P>0.05). The present study confirmed that experimental injection of 500 mg/kg/day D-GAL for 8 weeks to Wistar rats could lead to ABRs threshold shifts but not latency. Because there are several types of presbycusis, further studies are needed to determine what type of presbycusis is induced by D-GAL and where is the first region affected by it to provide the best treatment and prevention methods.
© 2019 Tehran University of Medical Sciences. All rights reserved.
Acta Med Iran 2019;57(5):281-288.
Association of Neonatal Asphyxia With Serum Levels of Heat Shock Protein 27 in a Small Sample of Newborns
Authors: Farhat Ahmadshah , Shafiee Mojtaba , Mohammadzadeh Ashraf , Saeidi Reza , Amiri Rana , Ghayour-Mobarhan Majid
Published: November 2019

Neonatal asphyxia is a state of hypoxia and hypercapnia caused by failure to breathe spontaneously and regularly soon after birth. Heat shock proteins (HSPs) are a ubiquitous and diverse group of highly conserved proteins which are rapidly up-regulated following periods of cellular stress including exposure to heat, ultraviolet irradiation, or chemical toxicity. The aim of the current study was to explore whether there is a relation between serum levels of HSP27 and neonatal asphyxia in a small sample of newborns. A total of 25 healthy newborns and 25 newborns diagnosed with neonatal asphyxia were recruited form Imam Reza Hospital, Mashhad, Iran. The Apgar score was recorded at one minute after delivery by trained nurses and newborns with the Apgar score of less than 7 were considered to be asphyctic. The mean birth weight of newborns in the case and control groups were 3110.47±613.5 g and 3230.4±584.83 g, respectively (P=0.4). Moreover, the mean maternal age of infants in the case group was higher than the mean maternal age of infants in the control group (31.1±6.1 vs. 30.1±5.0). Although it was marginally significant, the level of HSP27 was higher in the case group than the control group (0.23±0.08 vs. 0.19±0.09; P=0.07). Levels of HSP27 were found to be higher in newborns with neonatal asphyxia compared with healthy controls.
© 2019 Tehran University of Medical Sciences. All rights reserved.
Acta Med Iran 2019;57(5):303-307.
Non-Symmetric Bilateral Three Headed-Biceps Brachii
Authors: Kheirjou Raziyeh , Velaei Kobra , Ezzati Maryam
Published: November 2019

Anatomical muscles’ variation may face clinician and surgeon with some dilemmas. Obviously, reporting a rare case of variations could explore some unexplained and unexpected clinical symptoms. Bilateral three head biceps, which had a supernumerary head with different origins in both right and left arms, is reported in this case report article. The crucial importance of arm muscles variations’ is because of their adjacent location to brachial nerve plexus and brachial vessels. Every single alteration in the direction of anatomical elements could result in pressure on nerve or vessels with clinical symptoms. Taken together, investigation and popularization of variations make a well-knowledged background for clinicians and surgeons.
© 2019 Tehran University of Medical Sciences. All rights reserved.
Acta Med Iran 2019;57(5):338-340.
Preeclampsia y riesgo cardiovascular: estudio de seguimiento en la Población de GenPE en Colombia
Authors: Serrano-Díaz Norma Cecilia , Páez-Leal María Carolina , Beltrán-Avendaño Mónica Andrea , Colmenares-Mejìa Claudia Carolina , Guio-Mahecha Elizabeth , Bautista-Niño Paula , Lista completa al final del documento Colaboradores
Published: November 2019

Introducción: las mujeres con antecedente de preeclampsia (PE) tienen mayor riesgo de desarrollar enfermedad cardiovascular. El estudio GenPE ha captado gestantes con y sin PE en Colombia durante diez años, haciendo posible hoy evaluar desenlaces cardiovasculares en este grupo.Objetivo: determinar la presencia de enfermedad cardiovascular en mujeres jóvenes expuestas o no a preeclampsia, captadas por el estudio GenPE en Bucaramanga, Colombia.Materiales y métodos: estudio de cohorte que incluyó 106 pacientes –a partir de la cohorte original, 666 pacientes (2005-2010)–, menores de 26 años, primigestantes, sin antecedentes de enfermedades crónicas. Se realizó examen físico y venopunción para glucemia, perfil lipídico, ácido úrico y apolipoproteínas A-I y B. Se evaluó la presencia de hipertensión arterial (HA), obesidad, diabetes mellitus tipo 2 y síndrome metabólico. Se establecieron diferencias entre las mujeres expuestas y no expuestas a preeclampsia y el cambio entre el ingreso a GenPE y el primer seguimiento mediante test Wilcoxon o chi cuadrado y riesgo relativo con IC 95%.Resultados: se encontró asociación entre la exposición a PE con cifras mayores de presión arterial diastólica (p = 0,003) e hipercolesterolemia (p = 0,040). En toda la población existen niveles subóptimos de colesterol HDL y Apo A-I. Al primer seguimiento no se evidencia asociación con desenlaces fuertes, tales como: hipertensión arterial, obesidad, diabetes mellitus tipo 2, síndrome metabólico.Conclusión: en una muestra de mujeres colombianas jóvenes expuestas y no expuestas a PE, en un primer seguimiento, en los primeros dos años posteriores al parto, se evidencian cambios en la presión arterial diastólica (PAD), y biomarcadores asociados a riesgo cardiovascular.
La reivindicación por el derecho administrativo de la potestad disciplinaria
Authors: Molina Otero María Raquel
Published: November 2019

La obra reseñada presenta un estudio sólido y novedoso dentro de la tradición jurídica colombiana sobre la potestad disciplinaria de la Administración pública, reclamando su lugar como especialidad del derecho administrativo sancionador. De esta manera, se cuestiona el mito de que exista una disciplina autónoma llamada derecho disciplinario, ubicando sus orígenes dentro del derecho administrativo, y bajo la óptica de este último introduce una lectura adecuada de los principios aplicables, las categorías dogmáticas de la responsabilidad disciplinaria y los sujetos que intervienen en el proceso. Lo anterior, sin negar la importante relación que tiene esta especialidad del derecho administrativo con el derecho penal.
Relationship between spore fluctuations, environmental conditions and severity of calyx spot on roselle (Hibiscus sabdariffa)
Authors: Noriega-Cantú David H. , Toledo-Aguilar Rocío , Vasquez-Ortiz Romualdo , Alejo-Jaimes Antonino , Garrido-Ramírez Eduardo R. , Pereyda-Hernández Juan , González-Mateos Ricardo
Published: November 2019

La jamaica (Hibiscus sabdariffa) es afectada por severas enfermedades como tizón y manchado del cáliz (MCJ). El objetivo fue estudiar las relaciones entre fluctuación estacional de esporas de Corynespora y Coniella patógenos del manchado y tizón de cálices y hojas, las condiciones de clima y severidad (MCJ) en Ayutla, Gro., México. Las esporas fueron monitoreadas en dos ciclos, se aislaron los hongos y se realizó la identificación morfológica y molecular de Coniella el estudio se centró en patógenos reportados en México. Las pruebas de patogenicidad con C. diplodiella y C. cassiicola confirmaron la sintomatología muy similar entre ambos hongos. Coniella fue el más abundante (48%) y con menos cantidad Corynespora (8.7%). La dinámica de esporas tuvo una fluctuación mensual y relacionada con el clima. Las temperaturas máximas y medias (°C) y promedio de radiación global (W/m2) mostraron correlaciones positivas significativas con la abundancia de esporas de Coniella y el total de esporas, mientras que la precipitación (mm), temperatura mínima, velocidad del viento máxima (Km h-1) y horas de radiación solar, sus relaciones fueron negativas con las fluctuaciones de esporas de Corynespora. La severidad del manchado de hojas y cálices fue correlacionada positiva y significativa con el número de esporas capturadas.
Pencarian Pola Asosiasi Keluhan Pasien Menggunakan Teknik Association Rule Mining
Authors: Rosyidah Ulya Anisatur , Oktavianto Hardian
Published: November 2019

Perkembangan dan pertumbuhan data di bidang kesehatan semakin meningkat dan bertambah, baik dari kualitas maupun kuantitas, dilihat dari sisi kualitas, perkembangan data ini mengalami perubahan dari bentuk dokumen tulis menjadi dokumen digital atau yang biasanya kita sebut dengan file. Isu yang muncul adalah apakah informasi yang bisa diambil atau didapatkan dari sekian banyak data medis yang tersedia hanya berupa informasi – informasi pada umumnya, sedangkan dari suatu basis data yang tersedia seringkali memuat beberapa variabel sekaligus, bahkan apabila diteliti lebih jauh lagi, basis data yang berbeda bisa jadi memuat beberapa variabel yang sama, dari isu tersebut maka diperlukan suatu metode untuk bisa menggali lebih dalam informasi – informasi yang belum diketahui. Berkaitan dengan data medis serta data mining, maka penelitian kali ini akan membahas tentang implementasi atau kegunaan dari data mining pada data kunjungan pasien dengan cara menerapkan association rule mining untuk mendapatkan pola – pola asosiasi dari basis data kunjungan pasien yang tersedia menggunakan algoritma apriori dan algoritma FP-Growth. Baik algoritma apriori dan algoritma FP-Growth menghasilkan output yang sama. Perbedaan hasil uji coba terletak pada jumlah rule asosiasi yang ditemukan, dengan menggunakan algoritma apriori ditemukan 3 buah rule asosiasi, sedangkan ketika digunakan algoritma FP-Growth ditemukan 2 buah rule asosiasi, hal ini terjadi pada saat  uji coba yang dilakukan menggunakan confidence sebesar 80%.
Strategi Menemukan Jalan Keluar Labirin dengan Waktu Tercepat Menggunakan Metode DFS
Authors: Rahmasuci Mustika , S Husnul Hotimatus , Azizah Maulia , Wulandari Putri , A Diah Adistia , Bukhori Saiful
Published: November 2019

Depth-first search algorithm are blind search process and deepen search follow a single track until found goal. Maze is complicated network way and tortuous, also have many deadlock. Maze often become a challenge in a game like puzzle, which one there’s an object in start position have find way out on specify position. in this journal we’ll discuss about utilization DFS on maze and fastest time to find way out.
Pengaplikasian Web Pada Pendaftaran Siswa Baru Menggunakan Model Waterfall Pada Bimbel Cerdas Ceria
Authors: Santoso Tri
Published: November 2019

In the world of education, there is always an increase that must be made. The increase is not only in terms of science, but from various sides that support the education world. Every educational institution competes to do this to improve quality in providing education to their students. In this paper, the author takes a case study at Bimbel Cerdas Ceria Tangerang. The registration system at Bimbel Cerdas Ceria still uses a manual system. The system requires prospective students to come directly and do the registration process. Therefore, it is necessary to apply a new student registration web that is able to overcome the problem. The web application is made using the Waterfall method for the analysis stages. With the application of the web, prospective students do not need to come directly to register. Registration confirmation can use e-mail so that no one can confirm the registration. Payments also use ATM so parents don't need to come directly to just make payments.
Rekomendasi Pemilihan Produk Simpanan Tabungan Bank Menggunakan Metode Weighted Product (WP)
Authors: Purnomo Agus Sidiq , Ariyanti Jevi
Published: November 2019

Many banks in Indonesia, both public and private banks, offer different advantages to the customers. As a result, it is often difficult for the public to decide where to save their money. For this reason, the writer propose a decision support system to help recommend a bank as a smart solution for saving.

This study employed the Weighted Product (WP) with seven criteria, namely initial deposit, subsequent deposits, administrative costs, depositional balances, interest rates, age range, and facilities. This system can help the prospective customers determine the ideal savings place for the future needs. This study shows that the highest final value after calculation is 0.0320 for Batara of BTN bank, and the lowest value is 0.0132 for Tapres of BCA bank.
Sistem Informasi Distribusi Cabai Dengan Metode Distribution Requirements Planning (DRP) di Provinsi Jawa Timur
Authors: Mansur Nur Kholis , Bukhori Saiful , Juwita Oktalia
Published: November 2019

Chili production in East Java in 2016 reached nearly 2 million tons with details of 1 million tons of chili and large chili 939 thousand tons. The abundant amount of chili production is still the cause of inflation. The inflation factor is caused by several factors, one of which is the abundant amount of chili production but occurs in certain months or other causes are uneven distribution. The chili production center in East Java is still experiencing problems with distribution activities in meeting the demands of chili every city. Shipments carried out in East Java also do not have an optimal value to meet the number of requests. The poor delivery plan also caused errors in arranging inventory to make deliveries. The East Java government needs a way to analyze the distribution of chili by planning scheduling to improve the ability to fulfill chili demand using the Distribution Requirement Planning (DRP) method. Planning using this DRP method can produce an analysis of the gross needs and needs of chili in each city with the level of service used in the safety stock is 90%, with a normal distribution table that is equal to 1.28.
The Optimization of Tuna Fish and Banana Blossoms into Abon Modified Products in Puger District, Jember Regency
Authors: Rohmawati Ninna , Moelyaningrum Anita Dewi , Witcahyo Eri
Published: November 2019

Puger is the largest fish producing sub-district in Jember district, which also has a prevalence of high nutritional problems. During this time there are “mitra” who are a group of fish processors and marketers (POKLAHSAR) namely "Ekonomi Puger Bahari (EPB)" and "Duta Tongkol" who try to explore the potential of fish in Puger District. Tuna fish abon produced by “mitra” has a soft texture (srundeng like) so that it is less preferred by consumers therefore this paper argues that there's a need to add banana blossoms as a compound, does not have P-IRT (product permit), and the marketing of the product is not optimal. The author believes that if there is a need for development in terms of products implemented in several respects, among others: 1) Training on modified abon production; 2) Management of P-IRT; 3) Training in business financing and marketing management; 4) Production and marketing assistance. This activity can help partner problems. Expected targets and outcomes, namely: 1) Abon modified tuna and banana heart products that have P-IRT, high protein content is expected to be a solution to KEP nutritional problems; 2) The method of business financing management and appropriate marketing management has been achieved. In addition to directly, partners have marketed their products through social networks and resellers. Partners experience additional turnover and number of buyers; 3) Mastery of skills in the form of skills makes modification abon. This activity has an impact on up-dating science and technology in partners.

Keywords: abon modified; tuna fish; banana blossom; optimization.
Promotion of Can Macanan Kaduk Traditional Arts Through Audio Visual Technology
Authors: Raharsono A Lilik Slamet , Rahardjo Christanto Pudji
Published: November 2019

Jember Regency is one of the regencies in East Java Province. Here, the growth and development of the culture are extremely rapid and cultural diversity has spread through the southern and northern regions with its respective uniqueness. The southern region is famous for its Javanese culture and the northern region with its Madurese culture. This has led to a combination of the two cultures, resulting in a performing art that is the combination of Javanese and Madurese culture, known as Can Macanan Kaduk. This traditional performing art is an art in Jember that is still maintained by some people. It is needed to support the creation of this performing art and promote it through audio-visual marketing technology so that it will ultimately become widespread and will be better known by the other people outside of Jember. To realize the program objectives, coaching and mentoring has been carried out, regarding the performing arts management with the artists of Can Macanan Kaduk. We create audio-visual work for them to help promote Can Macanan Kaduk. The promotion materials are based on audio-visual technology, consisting of the documentary film, profile videos, and audio-visual advertisement. Through audio-visual promotion and online marketing in websites and offline marketing in DVDs, it is hoped that this traditional performing art, Can Macanan Kaduk, will be widely recognized and gain stronger attention from foreigners, becoming a traditional performing art that characterizes the cultural identity of the Jember Regency.

Key Words: Audio Visual Technology, Can Macan Kaduk, Promotion.
Penerapan Entrepreneural Marketing Pada Kelompok Usaha Ekonomi Kreatif (Creativepreneur) di Desa Pakis Dalam Upaya Penguatan Desa Pakis Sebagai Desa Sejahtera Mandiri (DSM)
Authors: Kartika Intan , Molasy Honest Dody , Magfiroh Illia Seldon
Published: November 2019

Universitas Jember memiliki kontribusi yang cukup besar dalam mengupayakan pengentasan kemiskinan di Desa Pakis. Bekerjasama dengan Kementerian Sosial Republik Indonesia, Universitas Jember telah menetapkan Desa Pakis sebagai Desa binaan yang mendapatkan program Desa Sejahtera Mandiri (DSM). Salah satu upaya yang bisa dilakukan untuk mengentaskan kemiskinan dan menciptakan kemandirian desa adalah dengan mendirikan Kelompok Usaha Ekonomi Kreatif (Creativepreneur). Tujuan pengabdian ini adalah Melakukan pendampingan dan edukasi kepada mitra Kelompok Ekonmi Kreatif Desa Pakis mengenai (a) pemasaran produk dengan menerapkan Entrepreneural Marketing, (b) pengolahan produk berbasis potensi pertanian lokal dan pembuatan kerajinan tangan berbasis limbah produk, (c) strategi pengembangan bisnis dan analisis usaha, (d) manajemen organisasi usaha dan masyarakat. Kegiatan pengabdian PPK mulai dilaksanakan pada bulan Oktober tahun 2018 sampai dengan awal Desember 2018. Metode pelaksanaan dilakukan melalui tahapan (1) identifikasi permasalahan mitra, (2) sosialisasi kegiatan, (3) edukasi  Entrepreneural Marketing, (4) pelatihan pembuatan kerajinan tangan, (5) pelatihan produk olahan berbasis potensi local, (6) edukasi analisis usaha, strategi pengembangan usaha serta manajemen organisasi dan masyarakat. Hasil pengabdian menunjukkan bahwa kegiatan pengabdian PPK memberikan dampak positif bagi mitra. Indikatornya terlihat pada adanya perubahan pemikiran dan sikap terkait dengan manajemen dan pengembangan usaha, antara lain Meningkatnya  pengetahuan dan ketrampilan untuk dapat lebih kreatif dalam memasarkan produk sehingga dapat meningkatkan pendapatan mitra yang nantinya akan menjadi mitra yang mandiri, Meningkatnya pengetahuan dan pemahaman tentang  Entrepreneural Marketing, Meningkatnya ketrampilan dan kreatifitas usaha dibidang kerajinan tangan dan pengolahan produk makanan berbasis potensi pertanian lokal, Meningkatnya kemampuan menyusun analisis usaha dan menetapkan strategi pengembangan usaha, dan Meningkatnya kesadaran pentingnya manajemen organisasi dan masyarakat.

Kata Kunci: Entrepreneural Marketing, Creativepreneur, Desa Pakis, Desa Sejahtera Mandiri
A Fully Integrated in vitro Diagnostic Microsystem for Pathogen Detection Developed Using a “3D Extensible” Microfluidic Design Paradigm
Authors: Geng Zhi , Gu Yin , Li Shanglin , Lin Baobao , Liu Peng
Published: November 2019

Microfluidics is facing critical challenges in the quest of miniaturizing, integrating, and automating in vitro diagnostics, including the increasing complexity of assays, the gap between the macroscale world and the microscale devices, and the diverse throughput demands in various clinical settings. Here a “3D extensible” microfluidic design paradigm that consists of a set of basic structures and unit operations was developed for constructing any application-specific assay. Four basic structures- check valve (in), check valve (out), double-check valve (in and out), and on-off valve, were designed to mimic basic acts in biochemical assays. By combining these structures linearly, a series of unit operations can be readily formed. We then proposed a “3D extensible” architecture to fulfill the needs of the function integration, the adaptive “world-to-chip” interface, and the adjustable throughput in the X, Y, and Z directions, respectively. To verify this design paradigm, we developed a fully integrated loop-mediated isothermal amplification microsystem that can directly accept swab samples and detect Chlamydia trachomatis automatically with a sensitivity one order higher than that of the conventional kit. This demonstration validated the feasibility of using this paradigm to develop integrated and automated microsystems in a less risky and more consistent manner.
Financial Performance Analysis of Distressed Banks in Ghana: Exploration of Financial Ratios and Z-score
Authors: Juabin Matey
Published: November 2019

A robust bank industry is a major player in the stability of an economy. By way of financial ratios and Z-score, the study analysed UT Bank’s financial performance prior to the recent bank sector reforms. Annual financials over a four year period were used. Debt management practices of UT Bank per the results obtained were quite on the hind side. Leverage and risk variables were poorly handled. UT Bank would have been unable to meet creditors’ claims considering the mean average values of debt-to-assets and debt-to equity ratios of 0.76 and 0.90 respectively. The entire bank sector will be put on sound footing on if credit management practices of individual banks are refreshed. The bank industry regulator should tighten its supervisory and monitoring role over banks to help detect early signs of non-performing banks. The study further recommends that statutory lending limits of banks be re-enforced to uphold the threshold of 10 percent for unsecured loans and 25 percentage for secured loans of net owned funds of the bank.
Antagonisme Bacillus terhadap Infeksi Layu Fusarium pada Bibit Pisang Hasil Kultur Jaringan
Authors: Hadiwiyono Hadiwiyono , Widyantoro Arief , Widono Salim
Published: November 2019

Fusarium wilt (Fusarium oxysporum f.sp. cubense) is an important disease in banana. Fusarium wilt was hard to control because the pathogen can survive in many kind of soils type although there is no host. Therefore, overcoming the disease is urgently needed such as biological control. The endophytic Bacillus of banana was begun to use as antagonist agent to the pathogen. This research aimed to study the mechanism of antagonism and physiological character of Bacillus. There were 27 Bacillus isolates examined in-vitro to test the production of IAA, HCN, chitinase, pectinase, and antagonism. The top ten isolates based on the test in vitro were used to test in planta. The research showed that Bacillus were able to produce IAA, HCN, chitinase, pectinase, and able to retard the growth of Foc colony. The application of isolat B25 on banana seedling could decrease the disease intensity but still unable to prevent the fusarium wilt infection.
Peran Waktu Inokulasi Meloidogyne dalam Meningkatkan Infeksi Patogen Busuk Pangkal pada Bawang Putih
Authors: Handayani Budi , Wiyono Hadi , Subagya Subagya
Published: November 2019

Basal rot was an important disease in garlic caused by Fusarium oxysporum f. sp. cepae (FOCe) and a major obstacle in garlic cultivation. Root knot nematodes (Meloidogyne) is endoparasitic nematodes causing plants become more susceptible to fungal pathogen infection. This research aimed to study the effect of double inoculation FOCe and Meloidogyne, and current inoculation against disease severity of basal root. The research was conducted through experimental procedures and prepared based on a randomized complete block design, with two factors consisting of three levels inoculation time. The first factor was FOCe and the second factor was Meloidogyne. The results showed that Meloidogyne presence could increase the disease severity of basal rot of garlic. Garlic was inoculated at 35 days after planting (dap) by Meloidogyne and FOCe cause the most severe disease of basal rot.
Potensi Jamur Perakaran sebagai Agens Pengendalian Hayati Penyakit Moler (Fusarium oxysporum f.sp. Cepae) pada Bawang Merah
Authors: Nugroho Andhika Wahyu , Hadiwiyono Hadiwiyono , Sudadi Sudadi
Published: November 2019

Desease that often occurs on the shallot is moler caused by Fusarium oxysporum f. sp. cepae (FOCe). This research was conducted to studied the potential of root-colonizing fungi to suppres moler disease on shallot. The researchs were divided into two steps that were laboratory test and screen house test used Completely Randomized Design (CRD). The treatments in screen house test were the combination of four fungi that had best capability to inhibit growth of FOCe. Each treatment was repeated three times. The result showed that root-colonizing fungi could reduce the moler disease but haven’t affect significantly growth component of shallot. Lowest disease intensity was 20% on the combination of AJ01, AJ12, AJ17, AJ18. Root-colonizing fungi have potention to decrease Moler disease.
Kualitas Indeks Tanah Sebagai Estimator dari Agroforestry Tanah Kesehatan di Tirtomoyo Sub-DAS, Wonogiri
Authors: Pramono Irfan Budi , Prahesti Renita Ratna
Published: November 2019

Soil is a vital resource for humans and soil quality degradation will affect people's lives, either directly or indirectly. Tirtomoyo Sub-watershed decreas ed soil quality on lands. Vegetative methods by agroforestry systems has been done as conservation to prevent further soil quality degradation. The study aims to determine the condition and the correlation between indicators of physics, chemistry and biology as well as the health status of land agroforestry at Tirtomoyo Sub-watersheld using Soil Quality Index calculation. Descriptive exploratory res earc h through soil survey, soil sampling loc ations are determined by stratified random sampling method and obtained 11 locations. Data analysis that used includes the correlation analysis and PCA. The results showed that soil health in agroforestry land of Tirtomoyo Sub -watershed in a low state, with the most influential s oil quality indicators are Base Saturation (BS), total N, available P and Cation Exchange Capacity (CEC).
Ketersediaan dan Serapan Mg Kacang Tanah Alfisol dengan Abu Vulkanik Kelud dan Pupuk Organik Amandemen
Authors: Suntoro Suntoro , Widjianto Hery , Handayani Tutik
Published: November 2019

As a result of volcanic eruptions led to the fall of the ash, but there is no much research conducted against nutrient content and the nutrient availability in soil. Disposals of volcanic ash and organic matter are expected fill Mg need in the land. Magnesium In Alfisol is still  sufficientless for peanuts. The purpose of this research is study of the impact of volcanic ash and organic fertilizer for the availability and uptake of peanuts Mg  in the Alfisol. The experiments did  in a greenhouse used completely randomized design with one factor. Data were analyzed with F test rate from 95%, and if the  treatments showed significant influenced were continued with rate duncan's multiple range test fence from 95%. The observed variables include the levels of chlorophyll, available Mg and uptake Mg. Research results show that the interaction from treatment chlorophyll content, available Mg and uptake  Mg. Based on the findings of the observations of volcanic ash disposals operating significantly impact the availability of magnesium.
Penggunaan Limbah Baglog Tiram dan Jenis Nutrisi Terhadap Pakcoy Pada Hidroponik Substrat
Authors: Arni Putri Retno Bandriyati , Sulistyo Trijono Djoko , Anwar Chairul
Published: November 2019

The increase of oyster mushroom farmers in wonogiri will be impact immediately on increasing waste baglog. One of the utilization is to be medium on hydroponic substrate for growing mustard greens and it will combined with charcoal and various types of nutrients. The research was conducted from October to December 2015 at the Greenhouse C and Laboratory EMPT UNS.the research employed completely random design (CRD) with two factor that is ratio of medium and various types of nutrients. The result of research showed that interaction between ratio of medium and various types affected significantly  the parameters of leaf area, leaf fresh weight and leaf biomass. Root fresh weight, root volume, root length and root biomass is only influenced by the type of nutrition with the ab mix is the most good in improving and supplying nutrients
Implementasi Sekolah Lapang Iklim dan Dosis Pupuk Terhadap Karakter Kimia Tanah dan Hasil Jagung
Authors: Ramadhani Rifqi , Komariah Komariah , Sumani Sumani , Ariyanto Dwi Priyo
Published: November 2019

Indonesian Agency for Meteorology Climatology and Geophysics Agency (BMKG) stated that the implementation of CFS program can increase the production significantly up to 30% (BMKG 2016). This research was conducted to know the influence of climate field school (CFS) and fertilizer dosage on soil chemical properties and corn yield. The study was conducted with a nested experimental design with two factors. Factor A has two levels: CFS Program (S1) and nonCFS (S2) and factor B was fertilizer dose. Factor B is nested on factor A as A (B). The fertilizer dose factor consists of 6 levels; T0 (without fertilizer); T1 (100% organic fertilizer); T2 (75% organic + 25% inorganic fertilizer); T3 (50% organic + 50% inorganic fertilizer), T4 (25% organic + 75% inorganic fertilizer), T5 (100% inorganic fertilizer). The results showed that CFS program increased available P, available K and soil pH, while fertilizer dose (50% organic fertilizer + 50% inorganic fertilizer) on CFS program yielded the highest soil organic matter (4.12%). CFS and fertilizer dose both did not affect the yield of maize significantly. However, the average yield of maize on CFS land is greater (6.50 tonha-1) compared to nonCFS (5.38 tonha-1). The treatment with 100% inorganic fertilizers) on CFS program gives highest yield (8.75 tonha-1).