bluuebunny/crossref_metadata_2025 huggingface dataset:
Physicochemical Properties of Ion Pairs of Biological Macromolecules
Authors: Iwahara Junji , Esadze Alexandre , Zandarashvili Levani
Published: October 2015

Ion pairs (also known as salt bridges) of electrostatically interacting cationic and anionic moieties are important for proteins and nucleic acids to perform their function. Although numerous three-dimensional structures show ion pairs at functionally important sites of biological macromolecules and their complexes, the physicochemical properties of the ion pairs are not well understood. Crystal structures typically show a single state for each ion pair. However, recent studies have revealed the dynamic nature of the ion pairs of the biological macromolecules. Biomolecular ion pairs undergo dynamic transitions between distinct states in which the charged moieties are either in direct contact or separated by water. This dynamic behavior is reasonable in light of the fundamental concepts that were established for small ions over the last century. In this review, we introduce the physicochemical concepts relevant to the ion pairs and provide an overview of the recent advancement in biophysical research on the ion pairs of biological macromolecules.
Molecular Process Producing Oncogene Fusion in Lung Cancer Cells by Illegitimate Repair of DNA Double-Strand Breaks
Authors: Seki Yoshitaka , Mizukami Tatsuji , Kohno Takashi
Published: October 2015

Constitutive activation of oncogenes by fusion to partner genes, caused by chromosome translocation and inversion, is a critical genetic event driving lung carcinogenesis. Fusions of the tyrosine kinase genes ALK (anaplastic lymphoma kinase), ROS1 (c-ros oncogene 1), or RET (rearranged during transfection) occur in 1%–5% of lung adenocarcinomas (LADCs) and their products constitute therapeutic targets for kinase inhibitory drugs. Interestingly, ALK, RET, and ROS1 fusions occur preferentially in LADCs of never- and light-smokers, suggesting that the molecular mechanisms that cause these rearrangements are smoking-independent. In this study, using previously reported next generation LADC genome sequencing data of the breakpoint junction structures of chromosome rearrangements that cause oncogenic fusions in human cancer cells, we employed the structures of breakpoint junctions of ALK, RET, and ROS1 fusions in 41 LADC cases as “traces” to deduce the molecular processes of chromosome rearrangements caused by DNA double-strand breaks (DSBs) and illegitimate joining. We found that gene fusion was produced by illegitimate repair of DSBs at unspecified sites in genomic regions of a few kb through DNA synthesis-dependent or -independent end-joining pathways, according to DSB type. This information will assist in the understanding of how oncogene fusions are generated and which etiological factors trigger them.
RETRACTED: Jin et al. A Robust WLS Power System State Estimation Method Integrating a Wide-Area Measurement System and SCADA Technology. Energies 2015, 8, 2769–2787
Authors: Energies Editorial Office
Published: October 2015

The corresponding author has requested the withdrawal of [...]
A Review of Approaches for Sensing, Understanding, and Improving Occupancy-Related Energy-Use Behaviors in Commercial Buildings
Authors: Rafsanjani Hamed , Ahn Changbum , Alahmad Mahmoud
Published: October 2015

Buildings currently account for 30–40 percent of total global energy consumption. In particular, commercial buildings are responsible for about 12 percent of global energy use and 21 percent of the United States’ energy use, and the energy demand of this sector continues to grow faster than other sectors. This increasing rate therefore raises a critical concern about improving the energy performance of commercial buildings. Recently, researchers have investigated ways in which understanding and improving occupants’ energy-consuming behaviors could function as a cost-effective approach to decreasing commercial buildings’ energy demands. The objective of this paper is to present a detailed, up-to-date review of various algorithms, models, and techniques employed in the pursuit of understanding and improving occupants’ energy-use behaviors in commercial buildings. Previous related studies are introduced and three main approaches are identified: (1) monitoring occupant-specific energy consumption; (2) Simulating occupant energy consumption behavior; and (3) improving occupant energy consumption behavior. The first approach employs intrusive and non-intrusive load-monitoring techniques to estimate the energy use of individual occupants. The second approach models diverse characteristics related to occupants’ energy-consuming behaviors in order to assess and predict such characteristics’ impacts on the energy performance of commercial buildings; this approach mostly utilizes agent-based modeling techniques to simulate actions and interactions between occupants and their built environment. The third approach employs occupancy-focused interventions to change occupants’ energy-use characteristics. Based on the detailed review of each approach, critical issues and current gaps in knowledge in the existing literature are discussed, and directions for future research opportunities in this field are provided.
Sensorless Speed Control with Initial Rotor Position Estimation for Surface Mounted Permanent Magnet Synchronous Motor Drive in Electric Vehicles
Authors: Wu Xuan , Wang Hui , Huang Shoudao , Huang Keyuan , Wang Li
Published: October 2015

The accurate information of the initial rotor position is very critical for successful starting of the Surface-mounted Permanent Magnet Synchronous Motor (SPMSM). In order to solve the problems of low accuracy and unreliability in the conventional estimation strategy, in this paper, an improved initial rotor position estimation strategy without any position sensor for SPMSM at standstill is proposed based on rectangular pulse voltage injection. In the work, when the second series of pulse voltages were applied. By the ways of strengthening the effect of weakening or strengthening magnetic fields and increasing the difference between each current of the vector. The improved strategy enhanced reliability and raised the initial position estimation accuracy from 7.5° to 1.875°. The improved strategy does not need any additional hardware. Experimental results demonstrate the validity and usefulness of the improved strategy.
Indirect Reciprocity with Optional Interactions and Private Information
Authors: Olejarz Jason , Ghang Whan , Nowak Martin
Published: October 2015

We consider indirect reciprocity with optional interactions and private information. A game is offered between two players and accepted unless it is known that the other person is a defector. Whenever a defector manages to exploit a cooperator, his or her reputation is revealed to others in the population with some probability. Therefore, people have different private information about the reputation of others, which is a setting that is difficult to analyze in the theory of indirect reciprocity. Since a defector loses a fraction of his social ties each time he exploits a cooperator, he is less efficient at exploiting cooperators in subsequent rounds. We analytically calculate the critical benefit-to-cost ratio above which cooperation is successful in various settings. We demonstrate quantitative agreement with simulation results of a corresponding Wright–Fisher process with optional interactions and private information. We also deduce a simple necessary condition for the critical benefit-to-cost ratio.
Lifetime Effective Dose Assessment Based on Background Outdoor Gamma Exposure in Chihuahua City, Mexico
Authors: Luevano-Gurrola Sergio , Perez-Tapia Angelica , Pinedo-Alvarez Carmelo , Carrillo-Flores Jorge , Montero-Cabrera Maria , Renteria-Villalobos Marusia
Published: October 2015

Determining ionizing radiation in a geographic area serves to assess its effects on a population’s health. The aim of this study was to evaluate the spatial distribution of the background environmental outdoor gamma dose rates in Chihuahua City. This study also estimated the annual effective dose and the lifetime cancer risks of the population of this city. To determine the outdoor gamma dose rate in air, the annual effective dose and the lifetime cancer risk, 48 sampling points were randomly selected in Chihuahua City. Outdoor gamma dose rate measurements were carried out by using a Geiger-Müller counter. Outdoor gamma dose rates ranged from 113 to 310 nGy·h−1. At the same sites, 48 soil samples were taken to obtain the activity concentrations of 226Ra, 232Th and 40K and to calculate their terrestrial gamma dose rates. Radioisotope activity concentrations were determined by gamma spectrometry. Calculated gamma dose rates ranged from 56 to 193 nGy·h−1. Results indicated that the lifetime effective dose of the inhabitants of Chihuahua City is on average 19.8 mSv, resulting in a lifetime cancer risk of 0.001. In addition, the mean of the activity concentrations in soil were 52, 73 and 1097 Bq·kg−1, for 226Ra, 232Th and 40K, respectively. From the analysis, the spatial distribution of 232Th, 226Ra and 40K is to the north, to the north-center and to the south of city, respectively. In conclusion, the natural background gamma dose received by the inhabitants of Chihuahua City is high and mainly due to the geological characteristics of the zone. From the radiological point of view, this kind of study allows us to identify the importance of manmade environments, which are often highly variable and difficult to characterize.
Meta-Analysis of Multiple Sclerosis Microarray Data Reveals Dysregulation in RNA Splicing Regulatory Genes
Authors: Paraboschi Elvezia , Cardamone Giulia , Rimoldi Valeria , Gemmati Donato , Spreafico Marta , Duga Stefano , Soldà Giulia , Asselta Rosanna
Published: October 2015

Abnormalities in RNA metabolism and alternative splicing (AS) are emerging as important players in complex disease phenotypes. In particular, accumulating evidence suggests the existence of pathogenic links between multiple sclerosis (MS) and altered AS, including functional studies showing that an imbalance in alternatively-spliced isoforms may contribute to disease etiology. Here, we tested whether the altered expression of AS-related genes represents a MS-specific signature. A comprehensive comparative analysis of gene expression profiles of publicly-available microarray datasets (190 MS cases, 182 controls), followed by gene-ontology enrichment analysis, highlighted a significant enrichment for differentially-expressed genes involved in RNA metabolism/AS. In detail, a total of 17 genes were found to be differentially expressed in MS in multiple datasets, with CELF1 being dysregulated in five out of seven studies. We confirmed CELF1 downregulation in MS (p = 0.0015) by real-time RT-PCRs on RNA extracted from blood cells of 30 cases and 30 controls. As a proof of concept, we experimentally verified the unbalance in alternatively-spliced isoforms in MS of the NFAT5 gene, a putative CELF1 target. In conclusion, for the first time we provide evidence of a consistent dysregulation of splicing-related genes in MS and we discuss its possible implications in modulating specific AS events in MS susceptibility genes.
Systemically Administered, Target Organ-Specific Therapies for Regenerative Medicine
Authors: Järvinen Tero , May Ulrike , Prince Stuart
Published: October 2015

Growth factors and other agents that could potentially enhance tissue regeneration have been identified, but their therapeutic value in clinical medicine has been limited for reasons such as difficulty to maintain bioactivity of locally applied therapeutics in the protease-rich environment of regenerating tissues. Although human diseases are treated with systemically administered drugs in general, all current efforts aimed at enhancing tissue repair with biological drugs have been based on their local application. The systemic administration of growth factors has been ruled out due to concerns about their safety. These concerns are warranted. In addition, only a small proportion of systemically administered drugs reach their intended target. Selective delivery of the drug to the target tissue and use of functional protein domains capable of penetrating cells and tissues could alleviate these problems in certain circumstances. We will present in this review a novel approach utilizing unique molecular fingerprints (“Zip/postal codes”) in the vasculature of regenerating tissues that allows target organ-specific delivery of systemically administered therapeutic molecules by affinity-based physical targeting (using peptides or antibodies as an “address tag”) to injured tissues undergoing repair. The desired outcome of targeted therapies is increased local accumulation and lower systemic concentration of the therapeutic payload. We believe that the physical targeting of systemically administered therapeutic molecules could be rapidly adapted in the field of regenerative medicine.
Identification and Validation of Aspartic Acid Semialdehyde Dehydrogenase as a New Anti-Mycobacterium Tuberculosis Target
Authors: Meng Jianzhou , Yang Yanhui , Xiao Chunling , Guan Yan , Hao Xueqin , Deng Qi , Lu Zhongyang
Published: October 2015

Aspartic acid semialdehyde dehydrogenase (ASADH) lies at the first branch point in the essential aspartic acid biosynthetic pathway that is found in bacteria and plants but is absent from animals. Mutations in the asadh gene encoding ASADH produce an inactive enzyme, which is lethal. Therefore, in this study, we investigated the hypothesis that ASADH represents a new anti-Mycobacterium tuberculosis (MTB) target. An asadh promoter-replacement mutant MTB, designated MTB::asadh, in which asadh gene expression is regulated by pristinamycin, was constructed to investigate the physiological functions of ASADH in the host bacteria. Bacterial growth was evaluated by monitoring OD600 and ASADH expression was analyzed by Western blotting. The results showed that the growth and survival of MTB::asadh was completely inhibited in the absence of the inducer pristinamycin. Furthermore, the growth of the mutant was rigorously dependent on the presence of the inducer in the medium. The starved mutant exhibited a marked reduction (approximately 80%) in the cell wall materials compared to the wild-type, in addition to obvious morphological differences that were apparent in scanning electron microscopy studies; however, with the addition of pristinamycin, the cell wall contents and morphology similar to those of the wild-type strain were recovered. The starved mutant also exhibited almost no pathogenicity in an in vitro model of infection using mouse macrophage J774A.1 cells. The mutant showed a concentration-dependent recovery of pathogenicity with the addition of the inducer. These findings implicate ASADH as a promising target for the development of novel anti-MTB drugs.
Streamlining the Pipeline for Generation of Recombinant Affinity Reagents by Integrating the Affinity Maturation Step
Authors: Huang Renhua , Gorman Kevin , Vinci Chris , Dobrovetsky Elena , Gräslund Susanne , Kay Brian
Published: October 2015

Often when generating recombinant affinity reagents to a target, one singles out an individual binder, constructs a secondary library of variants, and affinity selects a tighter or more specific binder. To enhance the throughput of this general approach, we have developed a more integrated strategy where the “affinity maturation” step is part of the phage-display pipeline, rather than a follow-on process. In our new schema, we perform two rounds of affinity selection, followed by error-prone PCR on the pools of recovered clones, generation of secondary libraries, and three additional rounds of affinity selection, under conditions of off-rate competition. We demonstrate the utility of this approach by generating low nanomolar fibronectin type III (FN3) monobodies to five human proteins: ubiquitin-conjugating enzyme E2 R1 (CDC34), COP9 signalosome complex subunit 5 (COPS5), mitogen-activated protein kinase kinase 5 (MAP2K5), Splicing factor 3A subunit 1 (SF3A1) and ubiquitin carboxyl-terminal hydrolase 11 (USP11). The affinities of the resulting monobodies are typically in the single-digit nanomolar range. We demonstrate the utility of two binders by pulling down the targets from a spiked lysate of HeLa cells. This integrated approach should be applicable to directed evolution of any phage-displayed affinity reagent scaffold.
Genome Editing Using Mammalian Haploid Cells
Authors: Horii Takuro , Hatada Izuho
Published: October 2015

Haploid cells are useful for studying gene functions because disruption of a single allele can cause loss-of-function phenotypes. Recent success in generating haploid embryonic stem cells (ESCs) in mice, rats, and monkeys provides a new platform for simple genetic manipulation of the mammalian genome. Use of haploid ESCs enhances the genome-editing potential of the CRISPR/Cas system. For example, CRISPR/Cas was used in haploid ESCs to generate multiple knockouts and large deletions at high efficiency. In addition, genome-wide screening is facilitated by haploid cell lines containing gene knockout libraries.
The Fungus among Us: Cryptococcus neoformans and Cryptococcus gattii Ecological Modeling for Colombia
Authors: Mak Sunny , Vélez Nórida , Castañeda Elizabeth , Escandón Patricia , Group Colombian
Published: October 2015

The environmental isolation of Cryptococcus spp. is typically a difficult undertaking. Collecting samples in the field is costly in terms of travel, personnel time and materials. Furthermore, the recovery rate of Cryptococcus spp. may be very low, thereby requiring a large number of samples to be taken without any guarantee of success. Ecological niche modeling is a tool that has traditionally been used to forecast the distribution of plant and animal of species for biodiversity and conservation purposes. Here, we use it in a public health application to produce risk area maps for cryptococcal disease in Colombia. The Genetic Algorithm for Ruleset Production (GARP) was used to create models for Cryptococcus neoformans (C. neoformans) and Cryptococcus gattii (C. gattii), based on environmental sampling and clinical records data recorded since 1987. These maps could be used to focus public health messaging related to cryptococcal disease, and it enables us to characterize the ecological niche for Cryptococcus in Colombia. We found that the OPEN ACCESS J. Fungi 2015, 1 333 ecological niche for C. gattii in Colombia is quite diverse, establishing itself in sub-tropical and temperate ecoregions within the country. This suggests that C. gattii is highly adaptive to different ecological conditions in Colombia and different regions of the world.
Modeling Percolation in Polymer Nanocomposites by Stochastic Microstructuring
Authors: Soto Matias , Esteva Milton , Martínez-Romero Oscar , Baez Jesús , Elías-Zúñiga Alex
Published: October 2015

A methodology was developed for the prediction of the electrical properties of carbon nanotube-polymer nanocomposites via Monte Carlo computational simulations. A two-dimensional microstructure that takes into account waviness, fiber length and diameter distributions is used as a representative volume element. Fiber interactions in the microstructure are identified and then modeled as an equivalent electrical circuit, assuming one-third metallic and two-thirds semiconductor nanotubes. Tunneling paths in the microstructure are also modeled as electrical resistors, and crossing fibers are accounted for by assuming a contact resistance associated with them. The equivalent resistor network is then converted into a set of linear equations using nodal voltage analysis, which is then solved by means of the Gauss–Jordan elimination method. Nodal voltages are obtained for the microstructure, from which the percolation probability, equivalent resistance and conductivity are calculated. Percolation probability curves and electrical conductivity values are compared to those found in the literature.
Bonding Properties of Basalt Fiber and Strength Reduction According to Fiber Orientation
Authors: Choi Jeong-Il , Lee Bang
Published: October 2015

The basalt fiber is a promising reinforcing fiber because it has a relatively higher tensile strength and a density similar to that of a concrete matrix as well as no corrosion possibility. This study investigated experimentally the bonding properties of basalt fiber with cementitious material as well as the effect of fiber orientation on the tensile strength of basalt fiber for evaluating basalt fiber’s suitability as a reinforcing fiber. Single fiber pullout tests were performed and then the tensile strength of fiber was measured according to fiber orientation. The test results showed that basalt fiber has a strong chemical bond with the cementitious matrix, 1.88 times higher than that of polyvinyl alcohol fibers with it. However, other properties of basalt fiber such as slip-hardening coefficient and strength reduction coefficient were worse than PVA and polyethylene fibers in terms of fiber bridging capacity. Theoretical fiber-bridging curves showed that the basalt fiber reinforcing system has a higher cracking strength than the PVA fiber reinforcing system, but the reinforcing system showed softening behavior after cracking.
Mechanical and Permeability Characteristics of Latex-Modified Pre-Packed Pavement Repair Concrete as a Function of the Rapid-Set Binder Content
Authors: Han Jae-Woong , Jeon Ji-Hong , Park Chan-Gi
Published: October 2015

We evaluated the strength and durability characteristics of latex-polymer-modified, pre-packed pavement repair concrete (LMPPRC) with a rapid-set binder. The rapid-set binder was a mixture of rapid-set cement and silica sand, where the fluidity was controlled using a latex polymer. The resulting mix exhibited a compressive strength of ¥21 MPa and a flexural strength of ¥3.5 MPa after 4 h of curing (i.e., the traffic opening term for emergency repairs of pavement). The ratio of latex polymer to rapid-set binder material was varied through 0.40, 0.33, 0.29, and 0.25. Mechanical characterization revealed that the mechanical performance, permeability, and impact resistance increased as the ratio of latex polymer to rapid-set binder decreased. The mixture exhibited a compressive strength of ¥21 MPa after 4 h when the ratio of latex polymer to rapid-set binder material was ¤0.29. The mixture exhibited a flexural strength of ¥3.5 MPa after 4 h when the ratio of latex polymer to rapid-set binder material was ¤0.33. The permeability resistance to chloride ions satisfied 2000 C after 7 days of curing for all ratios. The ratio of latex polymer to rapid-set binder material that satisfied all conditions for emergency pavement repair was ¤0.29.
Tangible Cooperative Gestures: Improving Control and Initiative in Digital Photo Sharing
Authors: Hoven Elise , Van Bergen Tom
Published: October 2015

This paper focuses on co-present digital photo sharing on a notebook and investigates how this could be supported. While analyzing the current digital photo sharing situation we noticed that there was a high threshold for visitors to take control of the personal computer of the photo owner, resulting in inequity of participation. It was assumed that visitors would have the opportunity to interact with the notebook more freely if this threshold was lowered by distributing the user interface and creating a more public, instead of personal, interaction space. This, in turn, could make them feel more involved and in control during a session, creating a more enjoyable experience. To test these assumptions a design prototype was created that stimulates participants to use tangible artifacts for cooperative gestures, a promising direction for the future of HCI. The situation with the cooperative gestures was compared with the regular digital photo sharing situation, which makes use of a keyboard. In dyads, visitors felt more involved and in control in the design prototype cooperative gestures condition (especially during storytelling), resulting in a more enjoyable digital photo sharing experience.
Bioactive Compounds Isolated from Microalgae in Chronic Inflammation and Cancer
Authors: Talero Elena , García-Mauriño Sofía , Ávila-Román Javier , Rodríguez-Luna Azahara , Alcaide Antonio , Motilva Virginia
Published: October 2015

The risk of onset of cancer is influenced by poorly controlled chronic inflammatory processes. Inflammatory diseases related to cancer development include inflammatory bowel disease, which can lead to colon cancer, or actinic keratosis, associated with chronic exposure to ultraviolet light, which can progress to squamous cell carcinoma. Chronic inflammatory states expose these patients to a number of signals with tumorigenic effects, including nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPK) activation, pro-inflammatory cytokines and prostaglandins release and ROS production. In addition, the participation of inflammasomes, autophagy and sirtuins has been demonstrated in pathological processes such as inflammation and cancer. Chemoprevention consists in the use of drugs, vitamins, or nutritional supplements to reduce the risk of developing or having a recurrence of cancer. Numerous in vitro and animal studies have established the potential colon and skin cancer chemopreventive properties of substances from marine environment, including microalgae species and their products (carotenoids, fatty acids, glycolipids, polysaccharides and proteins). This review summarizes the main mechanisms of actions of these compounds in the chemoprevention of these cancers. These actions include suppression of cell proliferation, induction of apoptosis, stimulation of antimetastatic and antiangiogenic responses and increased antioxidant and anti-inflammatory activity.
Pressure Induced Polymorphic Phase Transition of Natural Metamorphic Kalsilite; Electrical Resistivity and Infrared Spectroscopic Investigations
Authors: Parthasarathy G. , Santosh M.
Published: October 2015

We report here pressure dependence of the electrical resistivity of natural kalsilite (K0.998Na0.002Al0.998Fe0.002SiO4) from a granulite facies terrain in southern India. The electrical resistivity of kalsilite was measured with four probe technique up to 7.5 GPa at room temperature. The electrical resistivity decreases continuously with the increase of pressure up to 3.7 GPa, where there is a discontinuous drop in the electrical resistivity by 14%–16% indicating a first order transition. Further increase of pressure does not induce any phase transition up to 7.5 GPa at room temperature. Fourier transform infrared (FTIR) spectroscopy of the kalsilite sample at various pressures indicates that the observed transition is reversible in nature.
Ionic Liquid-Based Ultrasonic-Assisted Extraction of Secoisolariciresinol Diglucoside from Flaxseed (Linum usitatissimum L.) with Further Purification by an Aqueous Two-Phase System
Authors: Tan Zhi-Jian , Wang Chao-Yun , Yang Zi-Zhen , Yi Yong-Jian , Wang Hong-Ying , Zhou Wan-Lai , Li Fen-Fang
Published: October 2015

In this work, a two-step extraction methodology of ionic liquid-based ultrasonic-assisted extraction (IL-UAE) and ionic liquid-based aqueous two-phase system (IL-ATPS) was developed for the extraction and purification of secoisolariciresinol diglucoside (SDG) from flaxseed. In the IL-UAE step, several kinds of ILs were investigated as the extractants, to identify the IL that affords the optimum extraction yield. The extraction conditions such as IL concentration, ultrasonic irradiation time, and liquid–solid ratio were optimized using response surface methodology (RSM). In the IL-ATPS step, ATPS formed by adding kosmotropic salts to the IL extract was used for further separation and purification of SDG. The most influential parameters (type and concentration of salt, temperature, and pH) were investigated to obtain the optimum extraction efficiency. The maximum extraction efficiency was 93.35% under the optimal conditions of 45.86% (w/w) IL and 8.27% (w/w) Na2SO4 at 22 °C and pH 11.0. Thus, the combination of IL-UAE and IL-ATPS makes up a simple and effective methodology for the extraction and purification of SDG. This process is also expected to be highly useful for the extraction and purification of bioactive compounds from other important medicinal plants.
Comparative Studies on Phenolic Composition, Antioxidant, Wound Healing and Cytotoxic Activities of Selected Achillea L. Species Growing in Turkey
Authors: Agar Osman , Dikmen Miris , Ozturk Nilgun , Yilmaz Mustafa , Temel Hamdi , Turkmenoglu Fatma
Published: October 2015

Turkey is one of the most important centers of diversity for the genus Achillea L. in the world. Keeping in mind the immense medicinal importance of phenols, in this study, three species growing in Turkey, A. coarctata Poir. (AC), A. kotschyi Boiss. subsp. kotschyi (AK) and A. lycaonica Boiss. & Heldr. (AL) were evaluated for their phenolic compositions, total phenolic contents (TPC), antioxidant properties, wound healing potencies on NIH-3T3 fibroblasts and cytotoxic effects on MCF-7 human breast cancer cells. Comprehensive LC-MS/MS analysis revealed that AK was distinctively rich in chlorogenic acid, hyperoside, apigenin, hesperidin, rutin, kaempferol and luteolin (2890.6, 987.3, 797.0, 422.5, 188.1, 159.4 and 121.2 µg analyte/g extract, respectively). The findings exhibited a strong correlation between TPC and both free radical scavenging activity and total antioxidant capacity (TAC). Among studied species, the highest TPC (148.00 mg GAE/g extract) and TAC (2.080 UAE), the strongest radical scavenging (EC50 = 32.63 μg/mL), the most prominent wound healing and most abundant cytotoxic activities were observed with AK. The results suggested that AK is a valuable source of flavonoids and chlorogenic acid with important antioxidant, wound healing and cytotoxic activities. These findings warrant further studies to assess the potential of AK as a bioactive source that could be exploited in pharmaceutical, cosmetics and food industries.
Plasma Pharmacokinetics of Polyphenols in a Traditional Japanese Medicine, Jumihaidokuto, Which Suppresses Propionibacterium acnes-Induced Dermatitis in Rats
Authors: Matsumoto Takashi , Matsubara Yousuke , Mizuhara Yasuharu , Sekiguchi Kyoji , Koseki Junichi , Tsuchiya Kazuaki , Nishimura Hiroaki , Watanabe Junko , Kaneko Atsushi , Maemura Kazuya , Hattori Tomohisa , Kase Yoshio
Published: October 2015

Most orally administered polyphenols are metabolized, with very little absorbed as aglycones and/or unchanged forms. Metabolic and pharmacokinetic studies are therefore necessary to understand the pharmacological mechanisms of polyphenols. Jumihaidokuto (JHT), a traditional Japanese medicine, has been used for treatment of skin diseases including inflammatory acne. Because JHT contains various types of bioactive polyphenols, our aim was to clarify the metabolism and pharmacokinetics of the polyphenols in JHT and identify active metabolites contributing to its antidermatitis effects. Orally administered JHT inhibited the increase in ear thickness in rats induced by intradermal injection of Propionibacterium acnes. Quantification by LC-MS/MS indicated that JHT contains various types of flavonoids and is also rich in hydrolysable tannins, such as 1,2,3,4,6-penta-O-galloyl glucose. Pharmacokinetic and antioxidant analyses showed that some flavonoid conjugates, such as genistein 7-O-glucuronide and liquiritigenin 7-O-glucuronide, appeared in rat plasma and had an activity to inhibit hydrogen peroxide-dependent oxidation. Furthermore, 4-O-methylgallic acid, a metabolite of Gallic acid, appeared in rat plasma and inhibited the nitric oxide reaction. JHT has numerous polyphenols; it inhibited dermatitis probably via the antioxidant effect of its metabolites. Our study is beneficial for understanding in vivo actions of orally administered polyphenol drugs.
Boko Haram: Religion and Violence in the 21st Century
Authors: Voll John
Published: October 2015

Boko Haram in Nigeria provides an important example of the combination of religion and violence in the conditions of the twenty-first century. It is both a movement in the pattern of religiously-justified violence and a significant representative of the emergence of new types of modern terrorism in recent years. This article examines both of these aspects of Boko Haram as an example of religious violence. In the general development of religiously justified violence, Boko Haram is the heir to a long jihad tradition in West Africa. Its emergence follows well-established patterns of older militant Muslim groups, but it also departs significantly from those patterns as it shapes itself as a movement in the patterns of contemporary, twenty-first century modes of religious violence. Boko Haram is also identified, in twenty-first century terms, as a religious terrorist organization. As a religious terrorist group, it fits the pattern of what David Rapoport calls the fourth wave—the religious wave—of modern terrorism. However, in the second decade of the twenty-first century, Boko Haram exhibits characteristics of a new style of religious terrorism that is more like the so-called Islamic State than the older type of terrorist organization of al-Qa’idah.
Frequency of Low Clouds in Taiwan Retrieved from MODIS Data and Its Relation to Cloud Forest Occurrence
Authors: Thies Boris , Groos Alexander , Schulz Martin , Li Ching-Feng , Chang Shih-Chieh , Bendix Jörg
Published: October 2015

The relationship between satellite-derived low cloud frequency and the occurrence of tropical montane cloud forest (TMCF) in Taiwan was investigated. From daily MODIS cloud mask products between 2003 and 2012 the low cloud class was extracted and mean low cloud frequency was calculated for Taiwan. This low cloud frequency map was blended with an existing plot-based vegetation classification for Taiwan to analyze the relationship between low cloud frequency and TMCF occurrence. Receiver operating characteristics curves and the area under the ROC curve (AUC) were used to analyze if a relationship exists. No relationship was found for all four TMCF types taken together (AUC = 0.61) and for the dominant TMCF type, Quercus montane evergreen broad-leaved cloud forest (AUC = 0.5). Strong relationships were found for the two spatially-restricted TMCF types, Fagus montane deciduous broad-leaved cloud forest (AUC = 0.91) and Pasania-Elaeocarpus montane evergreen broad-leaved forest (AUC = 0.84), as well as for the second dominant type Chamaecyparis montane mixed cloud forest (AUC = 0.74). The results show that low cloud frequency thresholds might be associated with specific cloud forest types in Taiwan. Further studies should incorporate information about cloud base height, cloud density, and cloud immersion time as well as satellite-based cloud frequency information with a higher temporal resolution. Combination with satellite-based land cover classifications for Taiwan would allow quasi-continuous observation of TMCF changes. Such knowledge would be the precondition for effective protective actions concerning this exceptional but threatened ecosystem.
A 4D Filtering and Calibration Technique for Small-Scale Point Cloud Change Detection with a Terrestrial Laser Scanner
Authors: Kromer Ryan , Abellán Antonio , Hutchinson D. , Lato Matt , Edwards Tom , Jaboyedoff Michel
Published: October 2015

This study presents a point cloud de-noising and calibration approach that takes advantage of point redundancy in both space and time (4D). The purpose is to detect displacements using terrestrial laser scanner data at the sub-mm scale or smaller, similar to radar systems, for the study of very small natural changes, i.e., pre-failure deformation in rock slopes, small-scale failures or talus flux. The algorithm calculates distances using a multi-scale normal distance approach and uses a set of calibration point clouds to remove systematic errors. The median is used to filter distance values for a neighbourhood in space and time to reduce random type errors. The use of space and time neighbours does need to be optimized to the signal being studied, in order to avoid smoothing in either spatial or temporal domains. This is demonstrated in the application of the algorithm to synthetic and experimental case examples. Optimum combinations of space and time neighbours in practical applications can lead to an improvement of an order or two of magnitude in the level of detection for change, which will greatly improve our ability to detect small changes in many disciplines, such as rock slope pre-failure deformation, deformation in civil infrastructure and small-scale geomorphological change.
Remote Sensing Analysis Techniques and Sensor Requirements to Support the Mapping of Illegal Domestic Waste Disposal Sites in Queensland, Australia
Authors: Glanville Katharine , Chang Hsing-Chung
Published: October 2015

Illegal disposal of waste is a significant management issue for contemporary governments with waste posing an economic, social, and environmental risk. An improved understanding of the distribution of illegal waste disposal sites is critical to enhance the cost-effectiveness and efficiency of waste management efforts. Remotely sensed data has the potential to address this knowledge gap. However, the literature regarding the use of remote sensing to map illegal waste disposal sites is incomplete. This paper aims to analyze existing remote sensing methods and sensors used to monitor and map illegal waste disposal sites. The purpose of this paper is to support the evaluation of existing remote sensing methods for mapping illegal domestic waste sites in Queensland, Australia. Recent advances in technology and the acquisition of very high-resolution remote sensing imagery provide an important opportunity to (1) revisit established analysis techniques for identifying illegal waste disposal sites, (2) examine the applicability of different remote sensors for illegal waste disposal detection, and (3) identify opportunities for future research to increase the accuracy of any illegal waste disposal mapping products.
Hyperbolic Positioning with Antenna Arrays and Multi-Channel Pseudolite for Indoor Localization
Authors: Fujii Kenjirou , Sakamoto Yoshihiro , Wang Wei , Arie Hiroaki , Schmitz Alexander , Sugano Shigeki
Published: October 2015

A hyperbolic positioning method with antenna arrays consisting of proximately-located antennas and a multi-channel pseudolite is proposed in order to overcome the problems of indoor positioning with conventional pseudolites (ground-based GPS transmitters). A two-dimensional positioning experiment using actual devices is conducted. The experimental result shows that the positioning accuracy varies centimeter- to meter-level according to the geometric relation between the pseudolite antennas and the receiver. It also shows that the bias error of the carrier-phase difference observables is more serious than their random error. Based on the size of the bias error of carrier-phase difference that is inverse-calculated from the experimental result, three-dimensional positioning performance is evaluated by computer simulation. In addition, in the three-dimensional positioning scenario, an initial value convergence analysis of the non-linear least squares is conducted. Its result shows that initial values that can converge to a right position exist at least under the proposed antenna setup. The simulated values and evaluation methods introduced in this work can be applied to various antenna setups; therefore, by using them, positioning performance can be predicted in advance of installing an actual system.
A Context-Aware Model to Provide Positioning in Disaster Relief Scenarios
Authors: Moreno Daniel , Ochoa Sergio , Meseguer Roc
Published: October 2015

The effectiveness of the work performed during disaster relief efforts is highly dependent on the coordination of activities conducted by the first responders deployed in the affected area. Such coordination, in turn, depends on an appropriate management of geo-referenced information. Therefore, enabling first responders to count on positioning capabilities during these activities is vital to increase the effectiveness of the response process. The positioning methods used in this scenario must assume a lack of infrastructure-based communication and electrical energy, which usually characterizes affected areas. Although positioning systems such as the Global Positioning System (GPS) have been shown to be useful, we cannot assume that all devices deployed in the area (or most of them) will have positioning capabilities by themselves. Typically, many first responders carry devices that are not capable of performing positioning on their own, but that require such a service. In order to help increase the positioning capability of first responders in disaster-affected areas, this paper presents a context-aware positioning model that allows mobile devices to estimate their position based on information gathered from their surroundings. The performance of the proposed model was evaluated using simulations, and the obtained results show that mobile devices without positioning capabilities were able to use the model to estimate their position. Moreover, the accuracy of the positioning model has been shown to be suitable for conducting most first response activities.
Fiber-Optic Chemical Sensors and Fiber-Optic Bio-Sensors
Authors: Pospíšilová Marie , Kuncová Gabriela , Trögl Josef
Published: October 2015

This review summarizes principles and current stage of development of fiber-optic chemical sensors (FOCS) and biosensors (FOBS). Fiber optic sensor (FOS) systems use the ability of optical fibers (OF) to guide the light in the spectral range from ultraviolet (UV) (180 nm) up to middle infrared (IR) (10 μm) and modulation of guided light by the parameters of the surrounding environment of the OF core. The introduction of OF in the sensor systems has brought advantages such as measurement in flammable and explosive environments, immunity to electrical noises, miniaturization, geometrical flexibility, measurement of small sample volumes, remote sensing in inaccessible sites or harsh environments and multi-sensing. The review comprises briefly the theory of OF elaborated for sensors, techniques of fabrications and analytical results reached with fiber-optic chemical and biological sensors.
A High Performance LIA-Based Interface for Battery Powered Sensing Devices
Authors: García-Romeo Daniel , Valero María , Medrano Nicolás , Calvo Belén , Celma Santiago
Published: October 2015

This paper proposes a battery-compatible electronic interface based on a general purpose lock-in amplifier (LIA) capable of recovering input signals up to the MHz range. The core is a novel ASIC fabricated in 1.8 V 0.18 µm CMOS technology, which contains a dual-phase analog lock-in amplifier consisting of carefully designed building blocks to allow configurability over a wide frequency range while maintaining low power consumption. It operates using square input signals. Hence, for battery-operated microcontrolled systems, where square reference and exciting signals can be generated by the embedded microcontroller, the system benefits from intrinsic advantages such as simplicity, versatility and reduction in power and size. Experimental results confirm the signal recovery capability with signal-to-noise power ratios down to −39 dB with relative errors below 0.07% up to 1 MHz. Furthermore, the system has been successfully tested measuring the response of a microcantilever-based resonant sensor, achieving similar results with better power-bandwidth trade-off compared to other LIAs based on commercial off-the-shelf (COTS) components and commercial LIA equipment.
Depth Estimation of Submerged Aquatic Vegetation in Clear Water Streams Using Low-Altitude Optical Remote Sensing
Authors: Visser Fleur , Buis Kerst , Verschoren Veerle , Meire Patrick
Published: October 2015

UAVs and other low-altitude remote sensing platforms are proving very useful tools for remote sensing of river systems. Currently consumer grade cameras are still the most commonly used sensors for this purpose. In particular, progress is being made to obtain river bathymetry from the optical image data collected with such cameras, using the strong attenuation of light in water. No studies have yet applied this method to map submergence depth of aquatic vegetation, which has rather different reflectance characteristics from river bed substrate. This study therefore looked at the possibilities to use the optical image data to map submerged aquatic vegetation (SAV) depth in shallow clear water streams. We first applied the Optimal Band Ratio Analysis method (OBRA) of Legleiter et al. (2009) to a dataset of spectral signatures from three macrophyte species in a clear water stream. The results showed that for each species the ratio of certain wavelengths were strongly associated with depth. A combined assessment of all species resulted in equally strong associations, indicating that the effect of spectral variation in vegetation is subsidiary to spectral variation due to depth changes. Strongest associations (R2-values ranging from 0.67 to 0.90 for different species) were found for combinations including one band in the near infrared (NIR) region between 825 and 925 nm and one band in the visible light region. Currently data of both high spatial and spectral resolution is not commonly available to apply the OBRA results directly to image data for SAV depth mapping. Instead a novel, low-cost data acquisition method was used to obtain six-band high spatial resolution image composites using a NIR sensitive DSLR camera. A field dataset of SAV submergence depths was used to develop regression models for the mapping of submergence depth from image pixel values. Band (combinations) providing the best performing models (R2-values up to 0.77) corresponded with the OBRA findings. A 10% error was achieved under sub-optimal data collection conditions, which indicates that the method could be suitable for many SAV mapping applications.
GPS Cycle Slip Detection Considering Satellite Geometry Based on TDCP/INS Integrated Navigation
Authors: Kim Younsil , Song Junesol , Kee Changdon , Park Byungwoon
Published: October 2015

This paper presents a means of carrier phase cycle slip detection for an inertial-aided global positioning system (GPS), which is based on consideration of the satellite geometry. An integrated navigation solution incorporating a tightly coupled time differenced carrier phase (TDCP) and inertial navigation system (INS) is used to detect cycle slips. Cycle-slips are detected by comparing the satellite-difference (SD) and time-difference (TD) carrier phase measurements obtained from the GPS satellites with the range estimated by the integrated navigation solution. Additionally the satellite geometry information effectively improves the range estimation performance without a hardware upgrade. And the covariance obtained from the TDCP/INS filter is used to compute the threshold for determining cycle slip occurrence. A simulation and the results of a vehicle-based experiment verify the cycle slip detection performance of the proposed algorithm.