bluuebunny/crossref_metadata_2025 huggingface dataset:
Brassica oleracea Var italica by-Products Prevent Lipid Accumulation and Cell Death in a Liver Cell Model of Lipid Toxicity
Authors: Castelão-Baptista José P. , Valente Sara A. , Canário Sara , Oppolzer David , Barros Ana , Venâncio Carlos , Martins Tânia , Antunes Luís , Sardão Vilma A. , Rosa Eduardo , Oliveira Paulo J.
Published: February 2023

Obesity, a rising concern in the Eastern world, encompasses several co-morbidities, namely non-alcoholic fatty liver disease (NAFLD). Potential natural-based interventions to decrease the burden of obesity complications are being investigated. Many of the edible parts of plants are not sold for consumption and end up as massive waste, losing nutritional potential. In fact, a sizeable amount of waste is generated within the different steps of the food supply chain, representing a massive loss of both plant material and natural resources. A good example is Brassica by-products (BBPs). The objective of this work was to investigate the effect of three different extracts from broccoli (Brassica oleracea var italica) by-products in an in vitro model of free fatty acid (FFA)-induced lipotoxicity using human hepatoma HepG2 cells. Broccoli leaf, stalk, and inflorescence extracts induced a dose-dependent decrease in the cell viability of HepG2 cells. However, the maximal non-lethal concentrations of leaves, stalks, and inflorescences (10 μg/mL) did not compromise mitochondrial function or neutral lipid accumulation in HepG2 cells. The extracts significantly decreased FFA-induced lipid accumulation in HepG2 cells either in a co-incubation or pre-incubation strategy. The broccoli extracts’ capacity to prevent the FFA-induced decrease in catalase activity in HepG2 may explain the observed effects.
Association between Alcohol Intake and Prostate Cancer Mortality and Survival
Authors: D’Ecclesiis Oriana , Pastore Elisa , Gandini Sara , Caini Saverio , Marvaso Giulia , Jereczek-Fossa Barbara A. , Corrao Giulia , Raimondi Sara , Bellerba Federica , Ciceri Silvia , Latella Marialetizia , Cavalcabò Nora de Bonfioli , Bendinelli Benedetta , Saieva Calogero , Fontana Miriam , Gnagnarella Patrizia
Published: February 2023

We conducted a systematic review and meta-analysis to investigate the role of alcohol consumption with the prognosis of prostate cancer (PCa). Published reports were gathered on 15 October 2022, from PUBMED/MEDLINE and EMBASE. We found 19 independent eligible studies on the association between consumption of alcoholic beverages and the risk of fatal PCa (n = 5), PCa mortality (n = 5) in healthy subjects, and PCa patients’ survival (n = 7) or surrogates thereof (n = 2). We used random effects meta-analysis to obtain a summary risk estimate (SRE) and 95% confidence intervals (95%CI) for incidence of fatal PCa and PCa mortality. The meta-analysis revealed no association between alcohol consumption and fatal prostate cancer incidence risk in healthy subjects with an indication for publication bias, but omitting the study that mainly increased the between-study heterogeneity, the SRE becomes significant (SRE 1.33, 95%CI 1.12–1.58), and the heterogeneity disappeared (I2 = 0%) with no indication of publication bias. No association of alcohol consumption was found with mortality risk in PCa patients (SRE 0.97, 95%CI 0.92–1.03) and PCa mortality risk in healthy subjects (SRE 1.03, 95%CI 0.82–1.30). In conclusion, this study suggests that there is some evidence of an association between high alcohol consumption and an increased risk of incidence of fatal prostate cancer in healthy subjects. Given the inconsistencies this result warrants further confirmation.
Ketogenic Diet Applied in Weight Reduction of Overweight and Obese Individuals with Progress Prediction by Use of the Modified Wishnofsky Equation
Authors: Markovikj Gordana , Knights Vesna , Kljusurić Jasenka Gajdoš
Published: February 2023

Ketogenic diet is often used as diet therapy for certain diseases, among other things, its positive effect related to weight loss is highlighted. Precisely because of the suggestion that KD can help with weight loss, visceral obesity, and appetite control, 100 respondents joined the weight loss program (of which 31% were men and 69% were women). The aforementioned respondents were interviewed in order to determine their eating habits, the amount of food consumed, and the time when they consume meals. Basic anthropometric data (body height, body mass, chest, waist, hips, biceps, and thigh circumferences) were also collected, in order to be able to monitor their progress during the different phases of the ketogenic diet. Important information is the expected body mass during the time frame of a certain keto diet phase. This information is important for the nutritionist, medical doctor, as well as for the participant in the reduced diet program; therefore, the model was developed that modified the original equation according to Wishnofsky. The results show that women lost an average of 22.7 kg (average number of days in the program 79.5), and for men the average weight loss was slightly higher, 29.7 kg (with an average of 76.8 days in the program). The prediction of expected body mass by the modified Wishnofsky’s equation was extremely well aligned with the experimental values, as shown by the Bland-Altman graph (bias for women 0.021 kg and −0.697 kg for men) and the coefficient of determination of 0.9903. The modification of the Wishnofsky equation further shed light on the importance of controlled energy reduction during the dietetic options of the ketogenic diet.
Possible Combined Effects of Plasma Folate Levels, Global DNA Methylation, and Blood Cadmium Concentrations on Renal Cell Carcinoma
Authors: Huang Chao-Yuan , Chen Wei-Jen , Lee Hui-Ling , Lin Ying-Chin , Huang Ya-Li , Shiue Horng-Sheng , Pu Yeong-Shiau , Hsueh Yu-Mei
Published: February 2023

Epigenetic effects of environmental pollutants may be related to carcinogenesis. This study aimed to explore the association between the global DNA methylation marker: 5-methyl-2-deoxycytidine (5mdC) and renal cell carcinoma (RCC), and further investigated whether plasma folate and vitamin B12 levels and 5mdC modified the association between blood cadmium concentrations and RCC. We recruited 174 RCC patients and 673 non-RCC controls. Blood cadmium concentrations, plasma folate and vitamin B12 levels were measured. The amount of 5mdC in the DNA sample was expressed as percentages of the total cytosine content. An increase of 5mdC (%) and plasma folate and vitamin B12 levels were associated with decreasing odds ratio (OR) of RCC. Although plasma folate levels were not directly associated with 5mdC (%), a combined effect was observed with the odds of low plasma folate levels and low 5mdC (%) were greater among RCC patients compared to controls (OR (95% confidence interval, CI) = 11.86 (5.27–26.65)). Additionally, we observed that the odds of low plasma folate and high blood cadmium levels were greater among RCC patients than in controls (OR (95% CI): 8.15 (1.39–7.13)). This study provides suggestive evidence that plasma folate levels may modify the associations between 5mdC (%) or blood cadmium concentrations and RCC.
The Effect of Antenatal Education on Expectant Fathers’ Attitudes toward Breastfeeding and Attachment to the Fetus
Authors: Dagla Calliope , Antoniou Evangelia , Sarantaki Antigoni , Iliadou Maria , Mrvoljak-Theodoropoulou Irina , Andersson Ewa , Dagla Maria
Published: February 2023

Background: This study explores the effect of antenatal education on fathers’ attitudes toward: (i) breastfeeding and (ii) attachment to the fetus. A secondary aim is to explore the relationship of fathers’ demographic and the psycho-emotional characteristics that come with breastfeeding and attachment. Methods: This is a longitudinal study involving a group of 216 Greek expectant fathers who participated with their partners in an antenatal educational program performed by midwives in Athens, Greece (September 2020–November 2021). The Iowa Infant Feeding Attitudes Scale (IIFAS) and Paternal Antenatal Attachment Scale (PAAS) were administered at two time points: (a) 24th–28th gestation week and (b) 34th–38th gestation week. The T-test and Univariate Analyses of Variance (ANOVA) were performed. Results: The expectant fathers’ scores show that breastfeeding intention/exclusivity and prenatal attachment to the fetus were higher after their participation in the antenatal education program, but the difference was not statistically insignificant. Expectant fathers with a cohabitation agreement (p = 0.026), who felt very much supported by their partners (p = 0.001) and had no relationship difficulties with their partners (p < 0.001), as well as those who reported being very happy during pregnancy (p < 0.001), showed greater paternal antenatal attachment to the fetus. Conclusions: Although the difference was statistically insignificant, antenatal education appears to have an impact on paternal breastfeeding attitudes and antenatal attachment to the fetus. Additionally, several paternal characteristics were associated with greater antenatal attachment. Future research should be directed toward the investigation of additional factors that impact antenatal–paternal attachment and breastfeeding attitudes so that effective education programs can be designed.
Palladium Catalyzed Ring-Opening of Diazabicylic Olefins with 4-Halo-1,3-Dicarbonyl Compounds: Accessing 3(2H)-Furanone-Appended Cyclopentenes
Authors: Omanakuttan Vishnu K. , Valsan Alisha , Hopf Henning , John Jubi
Published: February 2023

We have realized a Pd-catalyzed ring-opening of diazabicyclic olefins with 4-halo-1,3-dicarbonyl compounds. This reaction resulted in the formation of 3(2H)-furanone-appended hydrazino cyclopentenes. The reaction proceeds via the formation of a π-allylpalladium intermediate which is attacked by the active methylene species, and an intramolecular nucleophilic substitution in the 4-halo-1,3-dicarbonyl moiety furnishes the 3(2H)-furanone-substituted cyclopentene. We could extend this methodology to cyclopropane-appended spirotricyclic olefin for synthesizing 3(2H)-furanone-substituted spiro[2.4]hept-5-ene.
Long-Lived Particles Anomaly Detection with Parametrized Quantum Circuits
Authors: Bordoni Simone , Stanev Denis , Santantonio Tommaso , Giagu Stefano
Published: February 2023

We investigate the possibility to apply quantum machine learning techniques for data analysis, with particular regard to an interesting use-case in high-energy physics. We propose an anomaly detection algorithm based on a parametrized quantum circuit. This algorithm was trained on a classical computer and tested with simulations as well as on real quantum hardware. Tests on NISQ devices were performed with IBM quantum computers. For the execution on quantum hardware, specific hardware-driven adaptations were devised and implemented. The quantum anomaly detection algorithm was able to detect simple anomalies such as different characters in handwritten digits as well as more complex structures such as anomalous patterns in the particle detectors produced by the decay products of long-lived particles produced at a collider experiment. For the high-energy physics application, the performance was estimated in simulation only, as the quantum circuit was not simple enough to be executed on the available quantum hardware platform. This work demonstrates that it is possible to perform anomaly detection with quantum algorithms; however, as an amplitude encoding of classical data is required for the task, due to the noise level in the available quantum hardware platform, the current implementation cannot outperform classic anomaly detection algorithms based on deep neural networks.
East Coast Fever Carrier Status and Theileria parva Breakthrough Strains in Recently ITM Vaccinated and Non-Vaccinated Cattle in Iganga District, Eastern Uganda
Authors: Oligo Stephen , Nanteza Ann , Nsubuga Julius , Musoba Abubakar , Kazibwe Anne , Lubega George Willy
Published: February 2023

East Coast fever (ECF) is a tick-borne disease of cattle that hinders the development of the livestock industry in eastern, central and southern Africa. The ‘Muguga cocktail’ live vaccine, delivered by an infection and treatment method (ITM), remains the only immunisation strategy of controlling ECF. However, there are challenges of the live vaccine inducing ECF carrier status in immunised animals and the possibility of lack of protection from parasite strains that are antigenically different from the vaccine strains. In Uganda, there are insufficient data regarding the ECF carrier status and T. parva genetic diversity in vaccinated and associated non-vaccinated cattle to assess the effectiveness of ITM vaccination. Blood was collected from recently ECF vaccinated (98) and non-vaccinated (73) cattle from Iganga district in Eastern Uganda at 120 days post-vaccination. The p104 gene nested PCR was used to screen for T. parva DNA, 11 minisatellite and 3 microsatellite markers (SSR) were used for genotyping. Two minisatellite markers (MS7 and MS19) were used to determine whether ECF carrier status was due to the T. parva vaccine or local strains. The prevalence of T. parva based on p104 nPCR was 61.2% (60/98) (RR 2.234, 95% CI 1.49–3.35, p-value < 0.001) among recently vaccinated cattle and 27.4% (20/73) (RR 1.00) among associated non-vaccinated cattle. The Muguga cocktail vaccine strains were responsible for carrier status in 10 (58.8%) by MS7 and 11 (64.7%) by MS19 in vaccinated cattle. Genotypes of T. parva with different-sized alleles to the vaccine strains that could be potential ‘breakthroughs’ were detected in 2 (11.8%)) and 4 (23.5%) isolates from vaccinated cattle based on MS7 and MS19 minisatellite markers, respectively. Using 14 SSR markers, T. parva diversity was higher in vaccinated (Na = 2.214, Ne = 1.978, He = 0.465) than associated non-vaccinated (Na = 1.071, Ne = 1.048, He = 0.259) cattle. The principal component analysis (PCA) showed isolates from vaccinated cattle were closely related to those from non-vaccinated cattle. The analysis of molecular variance (AMOVA) revealed high genetic variation (96%) within T. parva isolates from vaccinated and non-vaccinated cattle but low variation (4%) between vaccinated and non-vaccinated cattle. This study reveals the role of ITM in inducing the carrier status and higher T. parva genetic diversity in vaccinated cattle. The low genetic variation between T. parva isolates in both vaccinated and non-vaccinated cattle may be suggestive of the protective role of vaccine strains against genetically related local strains in the study area.
HPV and Cervical Cancer: A Review of Epidemiology and Screening Uptake in the UK
Authors: Choi Sunyoung , Ismail Ayden , Pappas-Gogos George , Boussios Stergios
Published: February 2023

Cervical cancer is the fourth most common malignancy in females worldwide, and a leading cause of death in the United Kingdom (UK). The human papillomavirus (HPV) is the strongest risk factor for developing cervical intraepithelial neoplasia and cancer. Across the UK, the national HPV immunisation programme, introduced in 2008, has been successful in protecting against HPV-related infections. Furthermore, the National Health Service (NHS) implemented the cytology-based cervical cancer screening service to all females aged 25 to 64, which has observed a decline in cervical cancer incidence. In the UK, there has been an overall decline in age-appropriate coverage since April 2010. In 2019, the COVID-19 pandemic disrupted NHS cancer screening and immunisation programmes, leading to a 6.8% decreased uptake of cervical cancer screening from the previous year. Engagement with screening has also been associated with social deprivation. In England, incidence rates of cervical cancer were reported to be 65% higher in the most deprived areas compared to the least, with lifestyle factors such as cigarette consumption contributing to 21% of cervical cancer cases. In this article, we provide an update on the epidemiology of cervical cancer, and HPV pathogenesis and transmission, along with the current prevention programmes within the NHS.
rMELEISH: A Novel Recombinant Multiepitope-Based Protein Applied to the Serodiagnosis of Both Canine and Human Visceral Leishmaniasis
Authors: Dias Daniel Silva , Machado Juliana Martins , Ribeiro Patrícia Aparecida Fernandes , Machado Amanda Sanchez , Ramos Fernanda Fonseca , Nogueira Lais Moreira , Gonçalves Ana Alice Maia , Ramos Luana de Sousa , Gandra Isadora Braga , Coutinho Flaviane Silva , Santos Michelli dos , Silva Jonatas Oliveira da , Chávez-Fumagalli Miguel Angel , Teixeira-Neto Rafael Gonçalves , Chaves Ana Thereza , Campos-da-Paz Mariana , Souza Amanda A. , Giunchetti Rodolfo Cordeiro , Freitas Sonia Maria , Lyon Sandra , de Magalhães-Soares Danielle Ferreira , Silveira Julia Angelica Gonçalves , Silva Eduardo Sergio , Coelho Eduardo Antonio Ferraz , Galdino Alexsandro Sobreira
Published: February 2023

Background: visceral leishmaniasis (VL) is a critical public health problem in over ninety countries. The control measures adopted in Brazil have been insufficient when it comes to preventing the spread of this overlooked disease. In this context, a precise diagnosis of VL in dogs and humans could help to reduce the number of cases of this disease. Distinct studies for the diagnosis of VL have used single recombinant proteins in serological assays; however, the results have been variable, mainly in relation to the sensitivity of the antigens. In this context, the development of multiepitope-based proteins could be relevant to solving such problem. Methods: a chimeric protein (rMELEISH) was constructed based on amino acid sequences from kinesin 39 (k39), alpha-tubulin, and heat-shock proteins HSP70 and HSP 83.1, and tested in enzyme-linked immunosorbent (ELISA) for the detection of L. infantum infection using canine (n = 140) and human (n = 145) sera samples. Results: in the trials, rMELEISH was able to discriminate between VL cases and cross-reactive diseases and healthy samples, with sensitivity and specificity values of 100%, as compared to the use of a soluble Leishmania antigenic extract (SLA). Conclusions: the preliminary data suggest that rMELEISH has the potential to be tested in future studies against a larger serological panel and in field conditions for the diagnosis of canine and human VL.
Neoehrlichia mikurensis—A New Emerging Tick-Borne Pathogen in North-Eastern Poland?
Authors: Szczotko Magdalena , Kubiak Katarzyna , Michalski Mirosław Mariusz , Moerbeck Leonardo , Antunes Sandra , Domingos Ana , Dmitryjuk Małgorzata
Published: February 2023

Neoehrlichia mikurensis is a new emerging tick-borne Gram-negative bacterium, belonging to the family Anaplasmataceae, the main vector of which in Europe is the tick Ixodes ricinus. N. mikurensis is responsible for neoehrlichiosis, occurring mostly in patients with underlying diseases. In the present study, a total of 348 I. ricinus and Dermacentor reticulatus ticks collected in north-eastern Poland were analyzed for the prevalence of N. mikurensis. A total of 140 questing ticks (124 of I. ricinus ticks and 16 D. reticulatus) collected with the flagging method and 208 ticks (105 and 103 I. ricinus and D. reticulatus, respectively) removed from dogs were selected for the study. cDNA (questing ticks) and total DNA (questing and feeding ticks) were analyzed by qPCR targeting the 16S rRNA gene of N. mikurensis. Positive samples were further analyzed by nested PCR and sequencing. The prevalence differed between ticks collected from vegetation (19.3%; 27/140) and ticks removed from dogs (6.7%; 14/208). The presence of the pathogen in questing and feeding D. reticulatus ticks was proven in Poland for the first time. In summary, our research showed that infections of ticks of both the most common tick species I. ricinus and D. reticulatus in north-eastern Poland are present and ticks collected from urban areas were more often infected than ticks from suburban and natural areas. The detection of N. mikurensis in I. ricinus and D. reticulatus ticks from north-eastern Poland indicates potential transmission risk for tick-bitten humans at this latitude.
In Vitro and In Silico Study on the Impact of Chlorogenic Acid in Colorectal Cancer Cells: Proliferation, Apoptosis, and Interaction with β-Catenin and LRP6
Authors: Vélez-Vargas Laura Catalina , Santa-González Gloria A. , Uribe Diego , Henao-Castañeda Isabel C. , Pedroza-Díaz Johanna
Published: February 2023

Colorectal cancer mortality rate and highly altered proteins from the Wnt/β-catenin pathway increase the scientific community’s interest in finding alternatives for prevention and treatment. This study aims to determine the biological effect of chlorogenic acid (CGA) on two colorectal cancer cell lines, HT-29 and SW480, and its interactions with β-catenin and LRP6 to elucidate a possible modulatory mechanism on the Wnt/β-catenin pathway. These effects were determined by propidium iodide and DiOC6 for mitochondrial membrane permeability, MitoTracker Red for mitochondrial ROS production, DNA content for cell distribution on cell cycle phases, and molecular docking for protein–ligand interactions and binding affinity. Here, it was found that CGA at 2000 µM significantly affects cell viability and causes DNA fragmentation in SW480 cells rather than in HT-29 cells, but in both cell lines, it induces ROS production. Additionally, CGA has similar affinity and interactions for LRP6 as niclosamide but has a higher affinity for both β-catenin sites than C2 and iCRT14. These results suggest a possible modulatory role of CGA over the Wnt/β-catenin pathway in colorectal cancer.
Novel Anticancer Strategies II
Authors: Bousbaa Hassan
Published: February 2023

Owing to the exceptional complexity of the development and progression of cancer, diverse cancer types are alarmingly increasing worldwide [...]
Pt(II)-PLGA Hybrid in a pH-Responsive Nanoparticle System Targeting Ovarian Cancer
Authors: Wlodarczyk Marek T. , Dragulska Sylwia A. , Chen Ying , Poursharifi Mina , Acosta Santiago Maxier , Martignetti John A. , Mieszawska Aneta J.
Published: February 2023

Platinum-based agents are the main treatment option in ovarian cancer (OC). Herein, we report a poly(lactic-co-glycolic acid) (PLGA) nanoparticle (NP) encapsulating platinum (II), which is targeted to a cell-spanning protein overexpressed in above 90% of late-stage OC, mucin 1 (MUC1). The NP is coated with phospholipid-DNA aptamers against MUC1 and a pH-sensitive PEG derivative containing an acid-labile hydrazone linkage. The pH-sensitive PEG serves as an off–on switch that provides shielding effects at the physiological pH and is shed at lower pH, thus exposing the MUC1 ligands. The pH-MUC1-Pt NPs are stable in the serum and display pH-dependent PEG cleavage and drug release. Moreover, the NPs effectively internalize in OC cells with higher accumulation at lower pH. The Pt (II) loading into the NP was accomplished via PLGA-Pt (II) coordination chemistry and was found to be 1.62 wt.%. In vitro screening using a panel of OC cell lines revealed that pH-MUC1-Pt NP has a greater effect in reducing cellular viability than carboplatin, a clinically relevant drug analogue. Biodistribution studies have demonstrated NP accumulation at tumor sites with effective Pt (II) delivery. Together, these results demonstrate a potential for pH-MUC1-Pt NP for the enhanced Pt (II) therapy of OC and other solid tumors currently treated with platinum agents.
Advanced Skin Antisepsis: Application of UVA-Cleavable Hydroxyethyl Starch Nanocapsules for Improved Eradication of Hair Follicle-Associated Microorganisms
Authors: Busch Loris , Hanuschik Anna Maria , Avlasevich Yuri , Darm Katrin , Hochheiser Elisa F. , Kohler Christian , Idelevich Evgeny A. , Becker Karsten , Rotsch Peter , Landfester Katharina , Darvin Maxim E. , Meinke Martina C. , Keck Cornelia M. , Kramer Axel , Zwicker Paula
Published: February 2023

Hair follicles constitute important drug delivery targets for skin antisepsis since they contain ≈25% of the skin microbiome. Nanoparticles are known to penetrate deeply into hair follicles. By massaging the skin, the follicular penetration process is enhanced based on a ratchet effect. Subsequently, an intrafollicular drug release can be initiated by various trigger mechanisms. Here, we present novel ultraviolet A (UVA)-responsive nanocapsules (NCs) with a size between 400 and 600 nm containing hydroxyethyl starch (HES) functionalized by an o-nitrobenzyl linker. A phase transfer into phosphate-buffered saline (PBS) and ethanol was carried out, during which an aggregation of the particles was observed by means of dynamic light scattering (DLS). The highest stabilization for the target medium ethanol as well as UVA-dependent release of ethanol from the HES-NCs was achieved by adding 0.1% betaine monohydrate. Furthermore, sufficient cytocompatibility of the HES-NCs was demonstrated. On ex vivo porcine ear skin, a strong UVA-induced release of the model drug sulforhodamine 101 (SR101) could be demonstrated after application of the NCs in cyclohexane using laser scanning microscopy. In a final experiment, a microbial reduction comparable to that of an ethanol control was demonstrated on ex vivo porcine ear skin using a novel UVA-LED lamp for triggering the release of ethanol from HES-NCs. Our study provides first indications that an advanced skin antisepsis based on the eradication of intrafollicular microorganisms could be achieved by the topical application of UVA-responsive NCs.
Reversal of Multidrug Resistance by Symmetrical Selenoesters in Colon Adenocarcinoma Cells
Authors: Rácz Bálint , Kincses Annamária , Laczi Krisztián , Rákhely Gábor , Domínguez-Álvarez Enrique , Spengler Gabriella
Published: February 2023

Recently, selenium containing derivatives have attracted more attention in medicinal chemistry. In the present work, the anticancer activity of symmetrical selenoesters was investigated by studying the reversal of efflux pump-related and apoptosis resistance in sensitive and resistant human colon adenocarcinoma cells expressing the ABCB1 protein. The combined effect of the compounds with doxorubicin was demonstrated with a checkerboard assay. The ABCB1 inhibitory and the apoptosis-inducing effects of the derivatives were measured with flow cytometry. Whole transcriptome sequencing was carried out on Illumina platform upon the treatment of resistant cells with the most potent derivatives. One ketone and three methyl ester selenoesters showed synergistic or weak synergistic interaction with doxorubicin, respectively. Ketone selenoesters were the most potent ABCB1 inhibitors and apoptosis inducers. Nitrile selenoesters could induce moderate early and late apoptotic processes that could be explained by their ABCB1 modulating properties. The transcriptome analysis revealed that symmetrical selenoesters may influence the redox state of the cells and interfere with metastasis formation. It can be assumed that these symmetrical selenocompounds possess toxic, DNA-damaging effects due to the presence of two selenium atoms in the molecule, which may be augmented by the presence of symmetrical groups.
Enhanced Tumor Accumulation of Low-Molecular-Weight Hyaluronic Acid/Chitosan Nanocomplexes for Photothermal Therapy
Authors: Jo Gayoung , Kim Eun Jeong , Hyun Hoon
Published: February 2023

Targeted phototheranostic nanosystems involving both cancer-specific near-infrared (NIR) fluorescence imaging and NIR light-induced phototherapy have shown great potential to improve cancer detection and treatment. In this study, a bifunctional nanocomplex based on low-molecular-weight hyaluronic acid (LMW-HA) and chitosan oligosaccharide lactate (COL) conjugating a zwitterionic NIR dye (ZW800-1) was rationally designed and prepared, and it was simultaneously used to enhance tumor accumulation and photothermal therapy (PTT). When HA-COL-ZW nanocomplexes were intravenously injected into mice bearing NCI-H460 tumors, HA-COL-ZW revealed increased tumor accumulation with prolonged tumor retention. Moreover, the ZW800-1 incorporated in HA-COL-ZW nanocomplexes showed excellent capability to convert NIR light into heat energy at the tumor site, acting as a PTT agent. Therefore, the targeted phototherapeutic HA-COL-ZW nanocomplex is a biocompatible and effective photothermal nanoagent, which could be a good candidate for future clinical use.
In Vitro Study of Biological Activity of Tanacetum vulgare Extracts
Authors: Babich Olga , Larina Viktoria , Krol Olesia , Ulrikh Elena , Sukhikh Stanislav , Gureev Maxim A. , Prosekov Alexander , Ivanova Svetlana
Published: February 2023

Tanacetum vulgare is an herbaceous plant widely used in folk medicine. It is rich in phenolic acids and flavonoids, which have pharmacological and medicinal properties, such as anthelmintic, antispasmodic, tonic, antidiabetic, diuretic, and antihypertensive. This study aimed to confirm the presence of biologically active substances in Tanacetum vulgare and to determine the pharmacological spectrum of biological activity of Tanacetum vulgare extract components. When preparing Tanacetum vulgare extracts, the highest yield was observed when using the maceration method with a mixture of solvents methanol + trifluoroacetic acid (22.65 ± 0.68%). The biologically active substances in Tanacetum vulgare extract samples were determined using high-performance liquid chromatography. Biologically active substances such as luteolin-7-glucoside (550.80 mg/kg), chlorogenic acid (5945.40 mg/kg), and rosmarinic acid (661.31 mg/kg) were identified. Their structures were determined. The experiments have confirmed the antioxidant and antibacterial activities. Secondary metabolites of Tanacetum vulgare extracts have been found to have previously unknown biological activity types; experimental confirmation of their existence will advance phytochemical research and lead to the development of new drugs.
Leveraging Physiologically Based Modelling to Provide Insights on the Absorption of Paliperidone Extended-Release Formulation under Fed and Fasting Conditions
Authors: Subhani Saima , Lukacova Viera , Kim Chaejin , Rodriguez-Vera Leyanis , Muniz Paula , Rodriguez Monica , Cristofoletti Rodrigo , Van Os Sandra , Suarez Elena , Schmidt Stephan , Vozmediano Valvanera
Published: February 2023

Paliperidone was approved by the US FDA in 2006 as an extended-release (ER) tablet (Invega®) for the once-daily treatment of schizophrenia. This osmotic-controlled release oral delivery system (OROS) offers advantages, such as the prevention of plasma concentration fluctuation and reduced dosing frequency. The administration of the ER after a high-fat/high-calorie meal leads to increased maximum plasma concentration and area under the curve values by 60% and 54%, respectively. Food has various effects on gastrointestinal (GI) physiology, including changed transit times, changed volumes, altered pH in different GI compartments, secretion of bile salts, and increased hepatic blood flow. This may affect solubility, the dissolution rate, absorption, and the pharmacokinetics. The aim of this study was to apply physiologically based absorption modeling (PBAM) to provide insights on paliperidone ER absorption under fed and fasting conditions. The PBAM adequately predicted absorption from the OROS formulation under both conditions. Absorption primarily occurs in the ascending colon and caecum. After a high-fat/high-calorie meal, absorption is increased through the jejunum, ileum, and colon due to either increased solubilization or the better efficiency of the OROS technology. PBAM-guided approaches can improve the understanding of branded drugs and thereby aid in guiding the development of generic formulations or formulation alternatives.
Green Synthesis of Blue-Emitting Graphene Oxide Quantum Dots for In Vitro CT26 and In Vivo Zebrafish Nano-Imaging as Diagnostic Probes
Authors: Gorle Govinda , Gollavelli Ganesh , Nelli Gowreeswari , Ling Yong-Chien
Published: February 2023

Graphene oxide quantum dots (GOQDs) are prepared using black carbon as a feedstock and H2O2 as a green oxidizing agent in a straightforward and environmentally friendly manner. The process adopted microwave energy and only took two minutes. The GOQDs are 20 nm in size and have stable blue fluorescence at 440 nm. The chemical characteristics and QD morphology were confirmed by thorough analysis using scanning electron microscope (SEM), transmission electron microscope (TEM), atomic force microscope (AFM), Fourier transmission infra-red (FT-IR), and X-ray photoelectron spectroscopy (XPS). The biocompatibility test was used to evaluate the toxicity of GOQDs in CT26 cells in vitro and the IC50 was found to be 200 µg/mL with excellent survival rates. Additional in vivo toxicity assessment in the developing zebrafish (Danio rerio) embryo model found no observed abnormalities even at a high concentration of 400 μg/mL after 96 h post fertilization. The GOQDs luminescence was also tested both in vitro and in vivo. They showed excellent internal distribution in the cytoplasm, cell nucleus, and throughout the zebrafish body. As a result, the prepared GOQDs are expected to be simple and inexpensive materials for nano-imaging and diagnostic probes in nanomedicine.
Research on Adjustable Ring-Mode Fiber Signal Combiner
Authors: Fu Min , Wang Jiawei , Li Zhixian , Yuan Weiyi , Wang Zefeng , Chen Zilun
Published: February 2023

Nowadays, fiber laser has been widely used in industrial processing, especially in welding, cutting and other fields, and the appearance of adjustable ring-mode fiber laser improves effectively the quality of processing. In this paper, a (6 + 1) × 1 adjustable ring-mode fiber signal combiner is developed based on the technology of fiber cladding corrosion. The test results show that under the same injection condition, the beam quality transmitted through the central port of the combiner is degraded by only 8.3% compared with that transmitted through a single 50/250 μm fiber. It is proven to be feasible to maintain the beam quality of the (6 + 1) × 1 combiner by fiber corrosion technology. In order to improve the power of the central port of the (6 + 1) × 1 adjustable ring-mode combiner, a 3 × 1 fiber signal combiner and the central port of (6 + 1) × 1 combiner are cascaded. The output beam quality is M2 = 4.45 and the overall transmission efficiency is greater than 95%. This combiner can choose the mode of the output beam according to the actual application requirements, so as to achieve a better application effect.
A Tunable Optoelectronic Oscillator with Phase-to-Amplitude Modulation Transformation via an Acetylene Reference Cell
Authors: Lebedev Vladimir , Agruzov Piotr , Iliyechev Igor’ , Varlamov Andrei , Tatsenko Ivan , Nikitin Andrey , Ustinov Alexey B. , Stepanov Serguei , Shamray Alexander
Published: February 2023

Operation modes of the optoelectronic oscillator (OEO), based on a phase electrooptic modulator with an acetylene reference cell as a photonic filter, have been investigated. For the wideband phase-to-amplitude demodulation that was observed when the laser wavelength was tuned to one side of the acetylene absorption line, an additional tunable electronic yttrium iron garnet (YIG) filter was introduced to observe single-mode OEO generation. This configuration generated a stable monochromatic signal in the 4–12 GHz frequency range, with phase noise of −122 dBc/Hz at 10 kHz offset from the carrier frequency. In the narrowband demodulation mode (when one optical modulation sideband was tuned to the acetylene absorption line), the quasi-single-mode oscillation could be observed without additional electronic filtering. In this case, the generation frequency was controlled optically by tuning the laser wavelength.
Low-Illumination Image Enhancement Based on Deep Learning Techniques: A Brief Review
Authors: Tang Hao , Zhu Hongyu , Fei Linfeng , Wang Tingwei , Cao Yichao , Xie Chao
Published: February 2023

As a critical preprocessing technique, low-illumination image enhancement has a wide range of practical applications. It aims to improve the visual perception of a given image captured without sufficient illumination. Conventional low-illumination image enhancement methods are typically implemented by improving image brightness, enhancing image contrast, and suppressing image noise simultaneously. Nevertheless, recent advances in this area are dominated by deep-learning-based solutions, and consequently, various deep neural networks have been proposed and applied to this field. Therefore, this paper briefly reviews the latest low-illumination image enhancement, ranging from its related algorithms to its unsolved open issues. Specifically, current low-illumination image enhancement methods based on deep learning are first sorted out and divided into four categories: supervised learning methods, unsupervised learning methods, semi-supervised learning methods, and zero-shot learning methods. Then, existing low-light image datasets are summarized and analyzed. In addition, various quality assessment indices for low-light image enhancement are introduced in detail. We also compare 14 representative algorithms in terms of both objective evaluation and subjective evaluation. Finally, the future development trend of low-illumination image enhancement and its open issues are summarized and prospected.
Yb-Doped Mode-Locked Fiber Laser Based on an All-Fiber Interferometer Filter
Authors: Yang Fan , Zhang Liqiang , Bai Chenglin , Ren Shijie , Tian Zhen , Yao Yicun , Wang Minghong
Published: February 2023

An interference filter is designed by fusing a segment of multi-core fiber (MCF) between two segments of multimode fibers (MMFs), which is then spliced between two segments of single mode fibers (SMFs). The light is split into the cladding and different cores of the MCF through the first segment of MMF, which is then coupled back into the core of SMF by the second segment of MMF. When the lengths of MCF are selected to be 4 mm and 10 mm, the 3 dB bandwidths of the filters around 1060 nm are 8.40 nm and 4.84 nm, respectively. Applying these filters in an Yb-doped fiber laser mode-locked by nonlinear polarization rotation, stable pulses have been obtained. Compared with the reported interference filters, the filter proposed in this paper has the advantages of simple fabrication process, compact structure and high environmental stability.
How Does Diurnal and Nocturnal Warming Affect the Freezing Resistance of Antarctic Vascular Plants?
Authors: López Dariel , Sanhueza Carolina , Salvo-Garrido Haroldo , Bascunan-Godoy Luisa , Bravo León A.
Published: February 2023

The Antarctic Peninsula has rapidly warmed up in past decades, and global warming has exhibited an asymmetric trend; therefore, it is interesting to understand whether nocturnal or diurnal warming is the most relevant for plant cold deacclimation. This study aimed to evaluate the effect of diurnal and nocturnal warming on Antarctic vascular plant’s freezing resistance under laboratory conditions. This was studied by measuring the lethal temperature for 50% of tissue (LT50), ice nucleation temperature (INT), and freezing point (FP) on Deschampsia antarctica and Colobanthus quitensis plants. Additionally, soluble carbohydrates content and dehydrin levels were analyzed during nocturnal and diurnal temperatures increase. Nocturnal warming led to a 7 °C increase in the LT50 of D. antarctica and reduced dehydrin-like peptide expression. Meanwhile, C. quitensis warmed plants reduce their LT50 to about 3.6 °C. Both species reduce their sucrose content by more than 28% in warming treatments. Therefore, nocturnal warming leads to cold deacclimation in both plant species, while C. quitensis plants are also cold-deacclimated upon warm days. This suggests that even when the remaining freezing resistance of both species allows them to tolerate summer freezing events, C. quitensis can reach its boundaries of freezing vulnerability in the near future if warming in the Antarctic Peninsula progress.
Variations in Soil Nitrogen Availability and Crop Yields under a Three-Year Annual Wheat and Maize Rotation in a Fluvo-Aquic Soil
Authors: Niu Runzhi , Zhu Changwei , Jiang Guiying , Yang Jin , Zhu Xuanlin , Li Lianyi , Shen Fengmin , Jie Xiaolei , Liu Shiliang
Published: February 2023

Optimum tillage practices can create a suitable soil environment, and they improve the soil nutrient status to ensure crop development and yield. In this study, we evaluated the influences of six tillage practices on soil nutrients and maize yields from 2017 to 2019 in fluvo-aquic soil in the North China Plain. The field experiment was carried out by a split design with rotary tillage (RT) and deep tillage (DT) in wheat season in the main plot and no-tillage (NT), subsoiling between the row (SBR), and subsoiling in the row (SIR) in maize season in the subplot. The results showed that the soil nutrient content was higher under the treatments with rotary tillage in the wheat season in the 0–20 cm soil layer, while in the 20–40 cm soil layer, the soil nutrient content was higher under the treatments with deep tillage in the wheat season. The integrated principal component scores indicated that the soil nutrients had improved in the second year. The ecosystem multifunctionality (EMF) index was higher with the treatments with rotary tillage in wheat season in the 0–20 cm soil layer, while it was the highest under DT-SIR at 20–40 cm. Correlation analysis showed that the soil EMF index correlated significantly (p < 0.05) with the soil nutrient content mainly in the 0–40 cm soil layer. The higher maize yield was under the treatments with deep tillage compared to that under the treatments with rotary tillage in the wheat season. The yield-increasing effect was higher under the treatments with subsoiling than those utilizing no-till in the maize season, with the highest average yield of 13,910 kg hm−2 in the DT-SIR during the three years. Maize yield was strongly correlated with nutrients in the subsoil layer. The higher yield stability was found under RT-NT. To sum up, during the three-year experiment, rotary tillage in the wheat season combined with subsoiling in the maize season improved the soil nutrient content and the EMF index in the 0–20 cm layer, while the combination of deep tillage in the wheat season and subsoiling in the maize season improved those indices in the 20–40 cm soil layer, and increased the maize yield, the best one was under DT-SIR.
Nitrogen Supplementation Modulates Morphological, Biochemical, Yield and Quality Attributes of Peppermint
Authors: Parrey Zubair Ahmad , Shah Sajad Hussain , Fayaz Mudasir , Casini Ryan , Elansary Hosam O. , Mohammad Firoz
Published: February 2023

Due to the rising demand for essential oil in the world market, peppermint has gained an important status among aromatic and medicinal plants. It becomes imperative to optimize its performance in terms of the growth, physiological functioning and biosynthesis of specialized metabolites. A factorial randomized pot experiment was performed using three peppermint cultivars (Kukrail, Pranjal and Tushar) and five levels of leaf-applied nitrogen (N), viz. 0 (control), 0.5, 1.0, 1.5 and 2%. The phenological features, biochemical parameters, viability of root cells, stomatal and trichome behavior were assessed at 100 days after transplanting (DAT). The yield-related parameters, viz., herbage yield, essential oil content, menthol content and yield were studied at 120 DAT. The results revealed that increasing the N doses up to 1.5% enhanced all the studied parameters of peppermint, which thereafter (at the dose above 1.5% N) decreased. The variation pattern of the studied parameters was “low-high-low”. Cultivar Kukrail surpassed the two other cultivars Tushar and Pranjal. Among the foliar sprays, the application of 1.5% N increased chlorophyll content and net photosynthetic rate in all three cultivars. Moreover, the essential oil (EO), EO yield and menthol yield of the plant were also increased linearly in all three cultivars as compared with their control plants. Nitrogen application enhanced the trichome size and density of the plants, as revealed through scanning electron microscopy. Furthermore, from the GC-MS studies, the EO content in the studied cultivars increased, particularly in the case of menthol, with the N application. It may be concluded that two sprays of N (1.5%) at appropriate growth stages could be beneficial for improving morphological, physio biochemical and yield attributes of peppermint.
Impact of the Alien Aquatic Plant Ludwigia hexapetala on the Native Utricularia australis: Evidence from an Indoor Experiment
Authors: Pelella Emanuele , Questino Beatrice , Ceschin Simona
Published: February 2023

Ludwigia hexapetala is an alien aquatic plant considered highly invasive in Europe since it alters freshwater habitats by forming dense mats both in water and along banks, outcompeting natives. Ludwigia effects on the native carnivorous plant Utricularia australis were investigated here. A 21-day indoor experiment was performed by setting up some separate tests in which Utricularia was made to grow both alone (control tests) and together with Ludwigia (arena tests). Water chemical and physical parameters and growth and morphological traits of Utricularia and Ludwigia were analysed weekly. Water samples were also analysed by UV-visible spectra to verify allelochemical (quercitrin) production by Ludwigia. In arena tests, oxygen concentration and pH were lower and conductivity higher than in control tests. Utricularia grew significantly less in arena tests in both shoot length and internode number, and its fresh weight, trap number and internode length decreased more than the control. Quercitrin was found only in arena tests as an allelochemical product released by Ludwigia. Overall, this study demonstrated Ludwigia significantly alters water parameters and negatively affects the growth of Utricularia, showing aggressive and competitive behaviour against this native species. Such evidence suggest that the widespread of L. hexapetala can represent a serious threat to the conservation of native plant diversity occurring in the freshwater habitats it invades.
The Type of Grain Counts: Effectiveness of Three Essential Oil-Based Nanoemulsions against Sitophilus oryzae
Authors: Kavallieratos Nickolas G. , Bonacucina Giulia , Nika Erifili P. , Skourti Anna , Georgakopoulou Stavroula Kyriaki C. , Filintas Constantin S. , Panariti Anna Maria E. , Maggi Filippo , Petrelli Riccardo , Ferrati Marta , Spinozzi Eleonora , Perinelli Diego Romano , Canale Angelo , Benelli Giovanni
Published: February 2023

Essential oil (EO)-based nanoemulsions (NEs) are promising grain protectants in the management of stored-product pests. However, the potential impact of the stored-grain species on the green insecticide effectiveness has been poorly studied. In this study, two concentrations of EO-based NEs from Carlina acaulis L., Mentha longifolia (L.) Huds., and Hazomalania voyronii (Jum.) Capuron were evaluated as insecticides against the major stored-product pest Sitophilus oryzae (L.) on barley, oats, and maize kernels. The C. acaulis EO-based NE applied at 1000 ppm on barley achieved the highest mortality, killing 94.4% of S. oryzae adults after a 7-day exposure, followed by 1000 ppm of H. voyronii EO-based NE (83.3%). The lowest mortality (1.1%) was recorded with 500 ppm of M. longifolia EO-based NE on maize after the same interval. All tested NEs exhibited elevated efficacy when applied on barley, while mortalities were lower on oats and maize. Furthermore, C. acaulis EO-based NE was the most effective when applied on all commodities, followed by H. voyronii and M. longifolia EO-based NEs. Overall, our results highlighted the significant impact of the stored cereal on the insecticidal effectiveness of EO-based NE used for stored-product pest control. Sitophilus oryzae adults on barley can be adequately controlled through the application of C. acaulis and H. voyronii EO-based NEs.