bluuebunny/crossref_metadata_2025 huggingface dataset:
Central Line-Associated Bloodstream Infection Due to Elizabethkingia anophelis: Case Report and Literature Review on Pediatric Infections
Authors: Maraki Sofia , Katzilakis Nikolaos , Neonakis Ioannis , Stafylaki Dimitra , Mavromanolaki Viktoria Eirini , Kyriakidis Ioannis , Pelagiadis Iordanis , Stiakaki Eftichia
Published: June 2024

Elizabethkingia anophelis is an opportunistic pathogen causing lifethreatening infections in humans, particularly in immunocompromised patients, neonates and the elderly. We report a case of central line-associated bloodstream infection by E. anophelis in a 2.5-year-old girl with acute lymphoblastic leukemia successfully treated with a combination of piperacillin/tazobactam and amikacin. The literature was also reviewed on pediatric infections caused by E. anophelis, focusing on clinical manifestations, underlying medical conditions, treatment and outcome. Accurate identification with MALDI-TOF, or using molecular techniques, is of the utmost importance because treatment and prognosis differ depending on the species. Considering that E. anophelis is multiresistant to antibiotics and that inappropriate antimicrobial therapy is an independent risk factor for mortality, the early, accurate identification of bacterial species and prompt effective treatment are essential to achieve optimal therapeutic outcomes.
Conofolidine: A Natural Plant Alkaloid That Causes Apoptosis and Senescence in Cancer Cells
Authors: Al-Hayali Mohammed Zuhair , Nge Choy-Eng , Lim Kuan Hon , Collins Hilary M. , Kam Toh-Seok , Bradshaw Tracey D.
Published: June 2024

Natural products contribute substantially to anticancer therapy; the plant kingdom provides an important source of molecules. Conofolidine is a novel Aspidosperma-Aspidosperma bisindole alkaloid isolated from the Malayan plant Tabernaemontana corymbosa. Herein, we report conofolidine’s broad-spectrum anticancer activity together with that of three other bisindoles—conophylline, leucophyllidine, and bipleiophylline—against human-derived breast, colorectal, pancreatic, and lung carcinoma cell lines. Remarkably, conofolidine was able to induce apoptosis (e.g., in MDA-MB-468 breast) or senescence (e.g., in HT-29 colorectal) in cancer cells. Annexin V-FITC/PI, caspase activation, and PARP cleavage confirmed the former while positive β-gal staining corroborated the latter. Cell cycle perturbations were evident, comprising S-phase depletion, accompanied by downregulated CDK2, and cyclins (A2, D1) with p21 upregulation. Confocal imaging of HCT-116 cells revealed an induction of aberrant mitotic phenotypes-membrane blebbing, DNA-fragmentation with occasional multi-nucleation. DNA integrity assessment in HCT-116, MDA-MB-468, MIAPaCa-2, and HT-29 cells showed increased fluorescent γ-H2AX during the G1 cell cycle phase; γ-H2AX foci were validated in HCT-116 and MDA-MB-468 cells by confocal microscopy. Conofolidine increased oxidative stress, preceding apoptosis- and senescence-induction in most carcinoma cell lines as seen by enhanced ROS levels accompanied by increased NQO1 expression. Collectively, we present conofolidine as a putative potent anticancer agent capable of inducing heterogeneous modes of cancerous cell death in vitro, encouraging further preclinical evaluations of this natural product.
High H2O-Assisted Proton Conduction in One Highly Stable Sr(II)-Organic Framework Constructed by Tetrazole-Based Imidazole Dicarboxylic Acid
Authors: Feng Junyang , Li Ying , Xie Lixia , Tong Jinzhao , Li Gang
Published: June 2024

Solid electrolyte materials with high structural stability and excellent proton conductivity (σ) have long been a popular and challenging research topic in the fuel cell field. This problem can be addressed because of the crystalline metal–organic frameworks’ (MOFs’) high structural stability, adjustable framework composition, and dense H-bonded networks. Herein, one highly stable Sr(II) MOF, {[Sr(H2tmidc)2(H2O)3]·4H2O}n (1) (H3tmidc = 2-(1H-tetrazolium-1-methylene)-1H-imidazole-4,5-dicarboxylic acid) was successfully fabricated, which was structurally characterized by single-crystal X-ray diffraction and electrochemically examined by the AC impedance determination. The results demonstrated that the σ of the compound manifested a positive dependence on temperature and humidity, and the optimal proton conductivity is as high as 1.22 × 10−2 S/cm under 100 °C and 98% relative humidity, which is at the forefront of reported MOFs with ultrahigh σ. The analysis of the proton conduction mechanism reveals that numerous tetrazolium groups, carboxyl groups, coordination, and crystallization water molecules in the framework are responsible for the high efficiency of proton transport. This work offers a fresh perspective on how to create novel crystalline proton conductive materials.
Simultaneous Formate and Syngas Conversion Boosts Growth and Product Formation by Clostridium ragsdalei
Authors: Schwarz Irina , Angelina Angelina , Hambrock Philip , Weuster-Botz Dirk
Published: June 2024

Electrocatalytic CO2 reduction to CO and formate can be coupled to gas fermentation with anaerobic microorganisms. In combination with a competing hydrogen evolution reaction in the cathode in aqueous medium, the in situ, electrocatalytic produced syngas components can be converted by an acetogenic bacterium, such as Clostridium ragsdalei, into acetate, ethanol, and 2,3-butanediol. In order to study the simultaneous conversion of CO, CO2, and formate together with H2 with C. ragsdalei, fed-batch processes were conducted with continuous gassing using a fully controlled stirred tank bioreactor. Formate was added continuously, and various initial CO partial pressures (pCO0) were applied. C. ragsdalei utilized CO as the favored substrate for growth and product formation, but below a partial pressure of 30 mbar CO in the bioreactor, a simultaneous CO2/H2 conversion was observed. Formate supplementation enabled 20–50% higher growth rates independent of the partial pressure of CO and improved the acetate and 2,3-butanediol production. Finally, the reaction conditions were identified, allowing the parallel CO, CO2, formate, and H2 consumption with C. ragsdalei at a limiting CO partial pressure below 30 mbar, pH 5.5, n = 1200 min−1, and T = 32 °C. Thus, improved carbon and electron conversion is possible to establish efficient and sustainable processes with acetogenic bacteria, as shown in the example of C. ragsdalei.
Vitamin D: An Essential Nutrient in the Dual Relationship between Autoimmune Thyroid Diseases and Celiac Disease—A Comprehensive Review
Authors: Gorini Francesca , Tonacci Alessandro
Published: June 2024

Autoimmune thyroid diseases (AITD) are among the most frequent autoimmune disorders, with a multifactorial etiology in which both genetic and environmental determinants are probably involved. Celiac disease (CeD) also represents a public concern, given its increasing prevalence due to the recent improvement of screening programs, leading to the detection of silent subtypes. The two conditions may be closely associated due to common risk factors, including genetic setting, changes in the composition and diversity of the gut microbiota, and deficiency of nutrients like vitamin D. This comprehensive review discussed the current evidence on the pivotal role of vitamin D in modulating both gut microbiota dysbiosis and immune system dysfunction, shedding light on the possible relevance of an adequate intake of this nutrient in the primary prevention of AITD and CeD. While future technology-based strategies for proper vitamin D supplementation could be attractive in the context of personalized medicine, several issues remain to be defined, including standardized assays for vitamin D determination, timely recommendations on vitamin D intake for immune system functioning, and longitudinal studies and randomized controlled trials to definitely establish a causal relationship between serum vitamin D levels and the onset of AITD and CeD.
Simultaneous Analysis of Thirteen Compounds in Yeokwisan Using High-Performance Liquid Chromatography–Photodiode Array Detection and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry and Their Antioxidant Effects
Authors: Seo Chang-Seob , Kim So-Yeon , Kim Dong-Seon
Published: June 2024

Yeokwisan (YWS) is an herbal medicine prescription consisting of six oriental herbal medicines, developed to treat reflux esophagitis. We focused on developing an analytical method capable of simultaneously quantifying 13 compounds in YWS samples using high-performance liquid chromatography–photodiode array detection (HPLC–PDA) and ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) and exploring their antioxidant effects. All compounds examined in both analytical systems were chromatographically separated on a SunFireTM C18 (4.6 × 250 mm, 5 μm) column and an Acquity UPLC BEH C18 (2.1 × 100 mm, 1.7 μm) column using gradient elution of a water–acetonitrile mobile phase. Antioxidant effects were evaluated based on radical scavenging activity (DPPH and ABTS tests) and ferrous ion chelating activity. In two analytical methods, the coefficient of determination of the regression equation was ≥0.9965, the recovery range was 81.11–108.21% (relative standard deviation (RSD) ≤ 9.33%), and the precision was RSD ≤ 11.10%. Application of the optimized analysis conditions gave quantitative analysis results for YWS samples of 0.02–100.36 mg/g. Evaluation of the antioxidant effects revealed that baicalein and baicalin exhibit significant antioxidant activity, suggesting that they play an important role in the antioxidant effects of YWS.
Effects of Foliar Boron Application on Physiological and Antioxidants Responses in Highbush Blueberry (Vaccinium corymbosum L.) Cultivars
Authors: Reyes-Díaz Marjorie , Cárcamo-Fincheira Paz , Tighe-Neira Ricardo , Nunes-Nesi Adriano , Savouré Arnould , Inostroza-Blancheteau Claudio
Published: June 2024

Boron (B) is a micronutrient crucial for the growth, development, productivity, and quality of crops. However, in areas characterized by acid soil (pHwater < 5.0) and high rainfall, soil B concentration tends to decrease, leading to insufficient supply to crops. This study was aimed at determining the optimal rate of B fertilization to enhance Vaccinium corymbosum L. performance in acid conditions. One-year-old cultivars with contrasting Al resistance (Al-sensitive Star and Al-resistant Cargo) were used. Plants were conditioned in plastic pots containing 18 L of half-ionic-strength Hoagland solution (pH 4.5) for 2 weeks. Thereafter, the following B treatments were applied foliarly: control, without B application (distilled water), 200, 400, and 800 mg L−1 of B as Solubor® for up to 72 h. Photosynthetic performance, root and shoot B levels, antioxidants, and oxidative stress were evaluated. Root and shoot B concentrations increased with the increasing B application, being higher in leaves than in roots of both cultivars. Net photosynthesis decreased at 800 mg L−1 B supply and effective quantum yield of PSII at 72 h in all B treatments. Lipid peroxidation increased in both cultivars at 800 mg L−1 B treatment. Antioxidant activity increased in all B treatments in both cultivars; while, at 400 and 800 mg L−1 B, total phenols increased in leaves of cultivar Star and decreased in cultivar Cargo. In conclusion, optimal B foliar application for highbush blueberry appears to be around 400 mg L−1 B. The appropriate B foliar application could help mitigate potential stress-induced problems in highbush blueberry cultivation. However, the optimal foliar B application should be confirmed in field experiments to help the farmers manage B nutrition.
Tomato Mutants Reveal Root and Shoot Strigolactone Involvement in Branching and Broomrape Resistance
Authors: Karniel Uri , Koch Amit , Bar Nun Nurit , Zamir Dani , Hirschberg Joseph
Published: June 2024

The phytohormones strigolactones (SLs) control root and shoot branching and are exuded from roots into the rhizosphere to stimulate interaction with mycorrhizal fungi. The exuded SLs serve as signaling molecules for the germination of parasitic plants. The broomrape Phelipanche aegyptiaca is a widespread noxious weed in various crop plants, including tomato (Solanum lycopersicum). We have isolated three mutants that impair SL functioning in the tomato variety M82: SHOOT BRANCHING 1 (sb1) and SHOOT BRANCHING 2 (sb2), which abolish SL biosynthesis, and SHOOT BRANCHING 3 (sb3), which impairs SL perception. The over-branching phenotype of the sb mutants resulted in a severe yield loss. The isogenic property of the mutations in a determinate growth variety enabled the quantitative evaluation of the contribution of SL to yield under field conditions. As expected, the mutants sb1 and sb2 were completely resistant to infection by P. aegyptiaca due to the lack of SL in the roots. In contrast, sb3 was more susceptible to P. aegyptiaca than the wild-type M82. The SL concentration in roots of the sb3 was two-fold higher than in the wild type due to the upregulation of the transcription of SL biosynthesis genes. This phenomenon suggests that the steady-state level of root SLs is regulated by a feedback mechanism that involves the SL signaling pathway. Surprisingly, grafting wild-type varieties on sb1 and sb2 rootstocks eliminated the branching phenotype and yield loss, indicating that SL synthesized in the shoots is sufficient to control shoot branching. Moreover, commercial tomato varieties grafted on sb1 were protected from P. aegyptiaca infection without significant yield loss, offering a practical solution to the broomrape crisis.
Cladanthus scariosus Essential Oil and Its Principal Constituents with Cytotoxic Effects on Human Tumor Cell Lines
Authors: Badalamenti Natale , Ilardi Vincenzo , Bruno Maurizio , Maggi Filippo , Quassinti Luana , Bramucci Massimo
Published: June 2024

Cladanthus is a small genus of the Asteraceae family comprising just five species that, apart from Cladanthus mixtus (L.) Chevall., has a large distribution in all the Mediterranean countries, mainly in the North Africa area. Several ethnopharmacological uses have been reported for species of this genus. Notably, Cladanthus scariosus (Ball) Oberpr. & Vogt is endemic to Morocco. Seeking to delve deeper into the phytochemistry and pharmacological aspects of this species, in this work, we investigated the essential oil (EO) obtained from the aerial parts of a locally sourced accession, hitherto unexplored, growing wild near Tizi n’Ticha, Morocco. The chemical composition of the EO, obtained by the hydrodistillation method, was evaluated by GC and GC-MS. The most abundant EO constituent was germacrene D (13.2%), the principal representative of the sesquiterpene hydrocarbons class (27.2%). However, the major class of constituents was monoterpene hydrocarbons (43.0%), with α-pinene (11.9%), sabinene (10.2%), p-cymene (8.5%), and α-phellandrene (5.2%) as the most abundant. The EO and its main constituents have been tested for their possible cytotoxic activity against three human tumor cell lines (MDA-MB 231, A375, and CaCo2) using the MTT assay, with corresponding IC50 values of 13.69, 13.21, and 22.71 µg/mL, respectively. Germacrene D and terpinen-4-ol were found to be the most active constituents with IC50 values between 3.21 and 9.53 µg/mL. The results demonstrate remarkable cytotoxic activity against the three human tumor cell lines studied, and in the future, further analyses could demonstrate the excellent potential of C. scariosus EO as an antitumor agent.
Upgrading Strategies for Managing Nematode Pests on Profitable Crops
Authors: Abd-Elgawad Mahfouz M. M.
Published: June 2024

Plant-parasitic nematodes (PPNs) reduce the high profitability of many crops and degrade their quantitative and qualitative yields globally. Traditional nematicides and other nematode control methods are being used against PPNs. However, stakeholders are searching for more sustainable and effective alternatives with limited side effects on the environment and mankind to face increased food demand, unfavorable climate change, and using unhealthy nematicides. This review focuses on upgrading the pre-procedures of PPN control as well as novel measures for their effective and durable management strategies on economically important crops. Sound and effective sampling, extraction, identification, and counting methods of PPNs and their related microorganisms, in addition to perfecting designation of nematode–host susceptibility/resistance, form the bases for these strategies. Therefore, their related frontiers should be expanded to synthesize innovative integrated solutions for these strategies. The latter involve supplanting unsafe nematicides with a new generation of safe and reliable chemical nematicidal and bionematicidal alternatives. For better efficacy, nematicidal materials and techniques should be further developed via computer-aided nematicide design. Bioinformatics devices can reinforce the potential of safe and effective biocontrol agents (BCAs) and their active components. They can delineate the interactions of bionematicides with their targeted PPN species and tackle complex diseases. Also, the functional plan of nematicides based on a blueprint of the intended goals should be further explored. Such goals can currently engage succinate dehydrogenase, acetylcholinesterase, and chitin deacetylase. Nonetheless, other biochemical compounds as novel targets for nematicides should be earnestly sought. Commonly used nematicides should be further tested for synergistic or additive function and be optimized via novel sequential, dual-purpose, and co-application of agricultural inputs, especially in integrated pest management schemes. Future directions and research priorities should address this novelty. Meanwhile, emerging bioactivated nematicides that offer reliability and nematode selectivity should be advanced for their favorable large-scale synthesis. Recent technological means should intervene to prevail over nematicide-related limitations. Nanoencapsulation can challenge production costs, effectiveness, and manufacturing defects of some nematicides. Recent progress in studying molecular plant–nematode interaction mechanisms can be further exploited for novel PPN control given related topics such as interfering RNA techniques, RNA-Seq in BCA development, and targeted genome editing. A few recent materials/techniques for control of PPNs in durable agroecosystems via decision support tools and decision support systems are addressed. The capability and effectiveness of nematicide operation harmony should be optimized via employing proper cooperative mechanisms among all partners.
Fabrication of k-Carrageenan/Alginate/Carboxymethyl Cellulose basedScaffolds via 3D Printing for Potential Biomedical Applications
Authors: Stavarache Cristina , Ghebaur Adi , Serafim Andrada , Vlăsceanu George Mihail , Vasile Eugeniu , Gârea Sorina Alexandra , Iovu Horia
Published: June 2024

Three-dimensional (3D) printing technology was able to generate great attention because of its unique methodology and for its major potential to manufacture detailed and customizable scaffolds in terms of size, shape and pore structure in fields like medicine, pharmaceutics and food. This study aims to fabricate an ink entirely composed of natural polymers, alginate, k-carrageenan and carboxymethyl cellulose (AkCMC). Extrusion-based 3D printing was used to obtain scaffolds based on a crosslinked interpenetrating polymer network from the alginate, k-carrageenan, carboxymethyl cellulose and glutaraldehide formulation using CaCl2, KCl and glutaraldehyde in various concentrations of acetic acid. The stabile bonding of the crosslinked scaffolds was assessed using infrared spectroscopy (FT-IR) as well as swelling, degradation and mechanical investigations. Moreover, morphology analysis (µCT and SEM) confirmed the 3D printed samples’ porous structure. In the AkCMC-GA objects crosslinked with the biggest acetic acid concentration, the values of pores and walls are the highest, at 3.9 × 10−2 µm−1. Additionally, this research proves the encapsulation of vitamin B1 via FT-IR and UV-Vis spectroscopy. The highest encapsulation efficiency of vitamin B1 was registered for the AkCMC-GA samples crosslinked with the maximum acetic acid concentration. The kinetic release of the vitamin was evaluated by UV-Vis spectroscopy. Based on the results of these experiments, 3D printed constructs using AkCMC-GA ink could be used for soft tissue engineering applications and also for vitamin B1 encapsulation.
Innovative Solution for Invasive Species and Water Pollution: Hydrochar Synthesis from Pleco Fish Biomass
Authors: Castro-Cárdenas Marisol , Medellín-Castillo Nahum Andrés , González-Fernández Lázaro Adrián , Leyva-Ramos Roberto , Gómez-Duran Cesar Fernando Azael , Gariepy Yvan , Pou K. R. Jolvis , Raghavan Vijaya
Published: June 2024

In recent years, the invasive pleco fish has emerged as a global concern due to its adverse effects on ecosystems and economic activities, particularly in various water bodies in Mexico. This study introduces an innovative solution, employing microwave-assisted hydrothermal carbonization (MHTC) to synthesize hydrochar from pleco fish biomass. The research aimed to optimize synthesis conditions to enhance hydrochar yield, calorific value, and adsorption capacities for fluoride and cadmium in water. MHTC, characterized by low energy consumption, high reaction rates, and a simple design, was employed as a thermochemical process for hydrochar production. Key findings revealed that through response surface analysis, the study identified the optimal synthesis conditions for hydrochar production, maximizing yield and adsorption capacities while minimizing energy consumption. Physicochemical characterization demonstrated that hydrochars derived from pleco fish biomass exhibited mesoporous structures with fragmented surfaces, resembling hydroxyapatite, a major component of bone. Hydrochars derived from pleco fish biomass exhibited promising adsorption capacities for fluoride and cadmium in water, with hydrochar from Exp. 1 (90 min, 160 °C) showing the highest adsorption capacity for fluoride (4.16 mg/g), while Exp. 5 (90 min, 180 °C) demonstrated superior adsorption capacity for cadmium (98.5 mg/g). Furthermore, the utilization of pleco fish biomass for hydrochar production not only offers an eco-friendly disposal method for invasive species but also addresses fluoride and cadmium contamination issues, contributing to sustainable waste management and water treatment solutions. The resulting hydrochar, rich in solid fuel content with low pollutant emissions, presents a promising approach for waste management and carbon sequestration. Moreover, the optimized synthesis conditions pave the way for sustainable applications in energy production, addressing critical environmental and public health concerns. This research provides valuable insights into the potential of microwave-assisted hydrothermal carbonization for transforming invasive species into valuable resources, thereby mitigating environmental challenges and promoting sustainable development.
Deep Learning Option Price Movement
Authors: Wang Weiguan , Xu Jia
Published: June 2024

Understanding how price-volume information determines future price movement is important for market makers who frequently place orders on both buy and sell sides, and for traders to split meta-orders to reduce price impact. Given the complex non-linear nature of the problem, we consider the prediction of the movement direction of the mid-price on an option order book, using machine learning tools. The applicability of such tools on the options market is currently missing. On an intraday tick-level dataset of options on an exchange traded fund from the Chinese market, we apply a variety of machine learning methods, including decision tree, random forest, logistic regression, and long short-term memory neural network. As machine learning models become more complex, they can extract deeper hidden relationship from input features, which classic market microstructure models struggle to deal with. We discover that the price movement is predictable, deep neural networks with time-lagged features perform better than all other simpler models, and this ability is universal and shared across assets. Using an interpretable model-agnostic tool, we find that the first two levels of features are the most important for prediction. The findings of this article encourage researchers as well as practitioners to explore more sophisticated models and use more relevant features.
LPI Sequences Optimization Method against Summation Detector Based on FFT Filter Bank
Authors: Liu Qiang , Guo Fucheng , Xiong Kunlai , Liu Zhangmeng , Hu Weidong
Published: June 2024

Waveform design is a crucial factor in electronic surveillance (ES) systems. In this paper, we introduce an algorithm that designs a low probability of intercept (LPI) radar waveform. Our approach directly minimizes the detection probability of summation detectors based on FFT filter banks. The algorithm is derived from the general quadratic optimization framework, which inherits the monotonic properties of such methods. To expedite overall convergence, we have integrated acceleration schemes based on the squared iterative method (SQUAREM). Additionally, the proposed algorithm can be executed through fast Fourier transform (FFT) operations, enhancing computational efficiency. With some modifications, the algorithm can be adjusted to incorporate spectral constraints, increasing its flexibility. Numerical experiments indicate that our proposed algorithm outperforms existing ones in terms of both intercept properties and computational complexity.
Digitalization, Sustainability, and Internationalization Nexus: Insights from Portuguese Entrepreneurs
Authors: Durão Natércia , Pereira Carla Santos , Lobo Carla , Moreira Fernando
Published: June 2024

The convergence of digitalization, sustainability, and internationalization represents a fundamental paradigm shift in today’s world of global business. This paper delves into the intricate relationship among these three pillars and their transformative impact on businesses, economies, and societies worldwide. Digitalization, characterized by the adoption of digital technologies, serves as a catalyst for internationalization, breaking down traditional barriers and fostering seamless connectivity across borders. Concurrently, the imperative of sustainability compels organizations to operate with consideration for environmental, social, and economic factors. Bearing this reality in mind, the aim of this study is to investigate, from the perspective of entrepreneurs of internationalized Portuguese companies, the factors contributing most to the importance they attach to digitalization in inducing internationalization. Using statistical methods of regression analysis (linear and logistic multivariate regression), the study seeks to identify the complex interactions between digitalization strategies and international expansion efforts. The results shed light on the critical role that digitalization plays in supporting internationalization goals and furthering sustainability objectives, stating that there is a strong connection between the importance of digitalization partnerships, counselling, and access to the new technologies and resources. Additionally, budget constraints may pose challenges for companies prioritizing digitalization efforts.
Control of Three-Dimensional Natural Convection of Graphene–Water Nanofluids Using Symmetrical Tree-Shaped Obstacle and External Magnetic Field
Authors: Aich Walid , Hilali-Jaghdam Inès , Alshahrani Amnah , Maatki Chemseddine , Alshammari Badr M. , Kolsi Lioua
Published: June 2024

This numerical investigation explores the enhanced control of the 3D natural convection (NC) within a cubic cavity filled with graphene–water nanofluids, utilizing a bottom-center-located tree-shaped obstacle and a horizontal magnetic field (MF). The analysis includes the effects of the Rayleigh number (Ra), the solid volume fraction of graphene (φ), the Hartmann number (Ha), and the fins’ length (W). The results show complex flow patterns and thermal behavior within the cavity, indicating the interactive effects of nanofluid properties, the tree-shaped obstacle, and magnetic field effects. The MHD effects reduce the convection, while the addition of graphene improves the thermal conductivity of the fluid, which enhances the heat transfer observed with increasing Rayleigh numbers. The increase in the fins’ length on the heat transfer efficiency is found to be slightly negative, which is attributed to the complex interplay between the enhanced heat transfer surface area and fluid flow disruption. This study presents an original combination of non-destructive methods (magnetic field) and a destructive method (tree-shaped obstacle) for the control of the fluid flow and heat transfer characteristics in a 3D cavity filled with graphene–water nanofluids. In addition, it provides valuable information for optimizing heat transfer control strategies, with applications in electronic cooling, renewable energy systems, and advanced thermal management solutions. The application of a magnetic field was found to reduce the maximum velocity and total entropy generation by about 82% and 76%, respectively. The addition of graphene nanoparticles was found to reduce the maximum velocity by about 5.5% without the magnetic field and to increase it by 1.12% for Ha = 100. Varying the obstacles’ length from W = 0.2 to W = 0.8 led to a reduction in velocity by about 23.6%.
Data Readout Techniques on FPGA for the ATLAS RPC-BIS78 Detectors
Authors: Vgenopoulos Andreas , Kordas Kostas , Lasagni Federico , Perrella Sabrina , Polini Alessandro , Vari Riccardo
Published: June 2024

The firmware developed for the readout and trigger processing of the information emerging from the BIS78-RPC Muon Spectrometer chambers in the ATLAS experiment at CERN is presented here, together with data processing techniques, data acquisition software, and tests of the readout chain system, which represent efforts to make these chambers operational in the ATLAS experiment. This work is performed in the context of the BIS78-RPC project, which deals with the pilot deployment of a new generation of sMDT+RPCs in the experiment. Such chambers are planned to be fully deployed in the whole barrel inner layer of the Muon Spectrometer during the Phase II upgrade of the ATLAS experiment. On-chamber front-ends include an amplifier, a discriminator ASIC, and an LVDS transmitter. The signal is digitized by CERN HPTDC chips and then processed by an FPGA, which is the heart of the readout and trigger processing, using various techniques.
Respiratory Virus-Induced PARP1 Alters DC Metabolism and Antiviral Immunity Inducing Pulmonary Immunopathology
Authors: Mire Mohamed , Elesela Srikanth , Morris Susan , Corfas Gabriel , Rasky Andrew , Lukacs Nicholas
Published: June 2024

Previous studies from our laboratory and others have established the dendritic cell (DC) as a key target of RSV that drives infection-induced pathology. Analysis of RSV-induced transcriptomic changes in RSV-infected DC revealed metabolic gene signatures suggestive of altered cellular metabolism. Reverse phase protein array (RPPA) data showed significantly increased PARP1 phosphorylation in RSV-infected DC. Real-time cell metabolic analysis demonstrated increased glycolysis in PARP1-/- DC after RSV infection, confirming a role for PARP1 in regulating DC metabolism. Our data show that enzymatic inhibition or genomic ablation of PARP1 resulted in increased ifnb1, il12, and il27 in RSV-infected DC which, together, promote a more appropriate anti-viral environment. PARP1-/- mice and PARP1-inhibitor-treated mice were protected against RSV-induced immunopathology including airway inflammation, Th2 cytokine production, and mucus hypersecretion. However, delayed treatment with PARP1 inhibitor in RSV-infected mice provided only partial protection, suggesting that PARP1 is most important during the earlier innate immune stage of RSV infection.
Impact of COVID-19 on Influenza and Pneumococcal Vaccination of Psoriatic Patients in Germany: Results from Vac-Pso
Authors: Kromer Christian , Wellmann Phoebe , Kromer Daniel , Patt Selina , Mohr Johannes , Wilsmann-Theis Dagmar , Mössner Rotraut
Published: June 2024

Background: Suboptimal influenza and pneumococcal vaccination rates have been reported before the COVID-19 pandemics in certain populations at risk for severe infection. The aim of this longitudinal cohort study was to investigate changes in influenza and pneumococcal vaccination rates and patient perceptions in patients with psoriasis (PsO) before and during the pandemic. Methods: Data on vaccination, patient and disease characteristics, comorbidity, and patient perceptions were collected with questionnaires before and during the pandemic approximately one year later. Results: Over the whole cohort who participated in the follow-up visit (n = 287; 59.2% male; mean age: 56.3 years), both influenza and pneumococcal lifetime vaccination prevalences increased significantly from 50.5% to 66.2% and from 16.0% to 41.5%, respectively. A total of 88.5% of PsO patients were interested in a COVID-19 vaccination or had already received it. The reasons for and against vaccinations changed significantly before and during the pandemic. Conclusions: Despite a promising increase in the vaccination prevalence in our PsO cohort, it remains important that awareness for vaccinations is encouraged and closely monitored in future research, particularly in populations at risk.
Porphyromonas gingivalis Vaccine: Antigens and Mucosal Adjuvants
Authors: Wang Shuo , Yan Tong , Zhang Bingtao , Chen Yixiang , Li Zhitao
Published: June 2024

Porphyromonas gingivalis (Pg), a Gram-negative anaerobic bacterium found in dental plaque biofilm within periodontal pockets, is the primary pathogenic microorganism responsible for chronic periodontitis. Infection by Pg significantly impacts the development and progression of various diseases, underscoring the importance of eliminating this bacterium for effective clinical treatment. While antibiotics are commonly used to combat Pg, the rise of antibiotic resistance poses a challenge to complete eradication. Thus, the prevention of Pg infection is paramount. Research suggests that surface antigens of Pg, such as fimbriae, outer membrane proteins, and gingipains, can potentially be utilized as vaccine antigens to trigger protective immune responses. This article overviews these antigens, discusses advancements in mucosal adjuvants (including immunostimulant adjuvants and vaccine-delivery adjuvants), and their application in Pg vaccine development. Furthermore, the review examines the advantages and disadvantages of different immune pathways and common routes of Pg vaccine immunization. By summarizing the current landscape of Pg vaccines, addressing existing challenges, and highlighting the potential of mucosal vaccines, this review offers new insights for the advancement and clinical implementation of Pg vaccines.
Exploring the Potentiality of a Plant Platform for Monoclonal Antibody Production in Veterinary Medicine
Authors: Morel Bertrand , Favrot Claude , Mirande Lucie , Grünwald-Gruber Clemens , Stordeur Virginie , Vezina Louis Philippe , Faye Loïc , Gomord Véronique
Published: June 2024

Canine atopic dermatitis (CAD) is an allergic, inflammatory, and pruritic skin disease associated with the production of IgE antibodies against environmental allergens and mainly house dust mite allergens. This complex dermatological pathology involves Interleukin 31 (IL-31) as a central itch mediator. One of the most effective CAD treatments is a caninized monoclonal antibody (mAb) called Lokivetmab. It is produced in CHO cells and targets specifically canine IL-31 (cIL-31) and blocks its cellular messaging. This treatment has undoubtedly contributed to a breakthrough in dermatitis-related pruritus. However, its production in mammalian cells requires time-consuming procedures, high production costs, and investment. Plants are considered an emerging protein production platform for recombinant biopharmaceuticals due to their cost-effectiveness and rapidity for production. Here, we use transient expression in Nicotiana benthamiana plants to produce recombinant canine Interleukin 31 (cIL-31) and an anti-IL-31 monoclonal antibody (M1). First, we describe the production and characterization of M1 and then its activity on an IL-31-induced pruritic model in dogs compared to its commercial homolog. Dogs treated with the plant-made M1 mAb have shown similar improvements to Lokivetmab-treated ones after different challenges using canine IL-31. Furthermore, M1 injections were not associated with any side effects. These results demonstrate the safety and efficacy of this plant-made Lokivetmab biosimilar to control dogs’ pruritus in a well-established model. Finally, this study shows that the plant-production platform can be utilized to produce rapidly functional mAbs and bring hope to the immunotherapy field of veterinary medicine.
Nonstructural Protein A238L of the African Swine Fever Virus (ASFV) Enhances Antiviral Immune Responses by Activating the TBK1-IRF3 Pathway
Authors: Liu Wei , Yang Lanlan , Di Chuanyuan , Sun Jing , Liu Penggang , Liu Huisheng
Published: June 2024

African swine fever virus (ASFV) is a double-stranded DNA virus with an envelope. ASFV has almost the largest genome among all DNA viruses, and its mechanisms of immune evasion are complex. Better understanding of the molecular mechanisms of ASFV genes will improve vaccine design. A238L, a nonstructural protein of ASFV, inhibits NF-κB activation by suppressing the HAT activity of p300. Whether A238L also affects the transcriptional activity of IRF3 remains unexplored. Here we first confirmed the ability of A238L to suppress NF-κB-activity in L929 cells. A238L inhibits the expression of proinflammatory cytokine genes. In contrast, A238L increased the phosphorylation levels of TBK1 and IRF3 in three different cell lines. A238L increases the IRF3-driven promoter activity and induces IRF3 nuclear translocation. Furthermore, A238L enhanced innate antiviral immunity in the absence or presence of poly d (A:T) or poly (I:C) stimulation, or herpes simplex virus type 1 (HSV-1) or Sendai virus (SeV) infection. This study reveals a previously unrecognized role of A238L in promoting antiviral immune responses by TBK1-IRF3 pathway activation.
Critical Review on Two-Stage Anaerobic Digestion with H2 and CH4 Production from Various Wastes
Authors: Zheng Xinyi , Li Ruying
Published: June 2024

Anaerobic digestion (AD) is a promising method for resource recovery from various wastes. Compared to the conventional single-stage AD process, a two-stage AD process with separate H2 and CH4 production provides higher energy recovery efficiency and enhanced operation stability. The stage separation makes it possible to apply optimal conditions for different functional microorganisms in their respective stages. This review elaborates the mechanisms of the two-stage AD process and evaluates recent research trends on this topic. A comprehensive comparison between single- and two-stage AD processes is made from the perspective of biogas production, organics degradation, energy recovery, and operation stability. The main influence factors on the two-stage AD process are discussed, including substrates, inoculum, and operation parameters, such as pH, temperature, etc. Upgrading technologies for the two-stage AD process are assessed. The microbial communities in the two-stage AD process for treating different substrates and the influence factors on microbial systems are also summarized. Furthermore, future research opportunities for enhancing the application of this technology are highlighted.
Correction: Simon et al. Wetland Management in Recharge Regions of Regional Groundwater Flow Systems with Water Shortage, Nyírség Region, Hungary. Water 2023, 15, 3589
Authors: Simon Szilvia , Déri-Takács Judit , Szijártó Márk , Szél László , Mádl-Szőnyi Judit
Published: June 2024

In the original publication [...]
A Comparative Study of Traffic Signal Control Based on Reinforcement Learning Algorithms
Authors: Ouyang Chen , Zhan Zhenfei , Lv Fengyao
Published: June 2024

In recent years, the increasing production and sales of automobiles have led to a notable rise in congestion on urban road traffic systems, particularly at ramps and intersections with traffic signals. Intelligent traffic signal control represents an effective means of addressing traffic congestion. Reinforcement learning methods have demonstrated considerable potential for addressing complex traffic signal control problems with multidimensional states and actions. In this research, the team propose Q-learning and Deep Q-Network (DQN) based signal control frameworks that use variable phase sequences and cycle times to adjust the order and the duration of signal phases to obtain a stable traffic signal control strategy. Experiments are simulated using the traffic simulator Simulation of Urban Mobility (SUMO) to test the average speed and the lane occupancy rate of vehicles entering the ramp to evaluate its safety performance and test the vehicle’s traveling time to assess its stability. The simulation results show that both reinforcement learning algorithms are able to control cars in dynamic traffic environments with higher average speed and lower lane occupancy rate than the no-control method and that the DQN control model improves the average speed by about 10% and reduces the lane occupancy rate by about 30% compared to the Q-learning control model, providing a higher safety performance.
All-Wheel Steering Tracking Control Method for Virtual Rail Trains with Only Interoceptive Sensors
Authors: Wang Zhenpo , Zhang Yi , Wang Zhifu
Published: June 2024

A virtual rail train (VRT) is a multi-articulated vehicle as well as a novel public transportation system due to its low economic cost, environmental friendliness and high transit capacity. Equipped with all-wheel steering (AWS) and a tracking control method, the super long VRT can travel on urban roads easily. This paper proposed a tracking control approach using only interoceptive sensors with high scene adaptivity. The kinematic model was established first under reasonable assumptions when the sensor configuration was completed simultaneously. A hierarchical controller consists of a front axle controller and a rear axle controller. The former applies virtual axles theory to avoid motion interference. The latter generates a first-axle reference path with path segmentation and a data updating method to improve storage and computational efficiency. Then, a fast curvature matching rear axles control method is developed with an actuator time delay considered. Finally, the proposed approach is verified in a hardware in loop (HIL) simulation under various situations with predefined evaluation standards, which shows better tracking performance and applicability.
PROBLEMS OF WASTE GAS HEAT UTILIZATION IN AUTONOMOUS BOILER HOUSES
Authors: Gubarev A.V.
Published: June 2024

The problem of fuel and energy resources is presented as one of the most important global problems. Energy saving in consumer heat supply systems is indicated as an important aspect of reducing the consumption of fuel and energy resources. There is a significant potential for energy saving in heat supply systems, which is conditioned by considerable heat losses with exhaust gases from boilers. A problems related to the utilization of low-potential heat of exhaust gases are identified. A review and analysis of ways to utilize the heat of the exhaust gases of boiler houses and directions of useful use of this heat are performed, their advantages and disadvantages are indicated. The direction of conducting research on methods of utilization of low-potential heat of exhaust gases and ways of using of this heat, allowing to create high-effective low-waste complexes and systems, is recommended.
Óvodáskorúak iróniaértése: A szülői nyelvhasználat mint befolyásoló tényező
Authors: Szabóné Habók Marianna
Published: June 2024

This study reports on a preliminary investigation exploring how the use of irony by parents might affect their preschool-aged children’s ability to understand verbal irony. The methodology involved a self-report questionnaire to gauge parents’ views on irony and a test to assess the children’s irony comprehension, both of which were based on the research of Banasik (2013) and Banasik-Jemielniak et al. (2020). The analysis showed a moderate positive correlation between the mothers’ irony use and their children’s level of irony comprehension. These results highlight the importance of children’s exposure to irony in their home environment as a considerable element in the development of their understanding of irony.
Shareholder Patience: Implications for Executive Compensation and Firm Performance
Authors: Alrashdan Abdullatif A.
Published: June 2024

Purpose: This paper explores how shareholder patience can influence firm performance and the efficacy of executive compensation and corporate governance in organizations. The shareholders’ patience, as reflected in their tendency to hold stock for extended periods, enables firm management to make long-term decisions and sustain ongoing project stability, which reflects positively on firm value. Shareholder patience amplifies the positive effects of un-exercisable options on a firm’s long-term performance and restrains overconfident CEOs by improving governance rather than by relying on market reactions, which positively influences performance.Study design/methodology/approach: The quantitative analysis conducted in this paper utilizes a fixed-effects regression model.Sample and data: This paper utilizes a multi-industry sample of 1,397 publicly traded U.S. firms that operated between 1999 and 2016. The data were retrieved from various archival sources.Results: Shareholders’ patience was positively associated with a firm’s performance, both in the short term and the long term. Further, the shareholders’ patience accentuated the positive effects of TMT un-exercisable stock options on the firm’s long-term performance but did not influence the negative effects of TMT exercisable stock options on the firm’s performance. Shareholder patience weakened the negative effects of CEO overconfidence on firm performance.Originality/value: This paper provides evidence of the effects of shareholders’ patience on the efficacy of executive compensation, which reflects positively on a firm’s performance.Research limitations/implications: The findings presented in this paper have limitations in terms of generalizability to non-U.S. firms and private and entrepreneurial firms.